doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text	PSource	NProtein	NID	SiteSource	SiteName
33857517	0	0	theme	in	80:81	arg1	bioprinting					91:101	in situ 3D bioprinting	80:101	in situ 3D bioprinting	80:101	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	2	1	theme	soluble	456:462	arg1	collagen					464:471	soluble collagen	456:471	soluble collagen in this study	456:485	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	4	2	theme	MHBC/1	843:848	arg1	%					849:849	%	849:849	%	849:849	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	1	3	with	bioink	213:218	arg1	biocompatibility					235:250	biocompatibility	235:250	biocompatibility	235:250	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	3	with	bioink	213:218	arg1	rheology					225:232	rheology	225:232	rheology	225:232	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	3	with	bioink	213:218	arg1	kinetics					266:273	gelation kinetics	257:273	gelation kinetics	257:273	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	3	4	theme	ratios	787:792	arg1	degree					764:769	the methacrylation degree	745:769	the methacrylation degree of MHBC and M/C ratios	745:792	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	1	5	theme	new	142:144	arg1	frontier					146:153	a new frontier	140:153	a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct	140:351	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	5	theme	new	142:144	arg1	technologies					124:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	0	6	theme	3D	88:89	arg1	bioprinting					91:101	in situ 3D bioprinting	80:101	in situ 3D bioprinting	80:101	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	5	7	theme	in	1112:1113	arg1	bioprinting					1123:1133	in situ 3D bioprinting	1112:1133	in situ 3D bioprinting	1112:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	4	8	dep	formulation	827:837	arg1	%					841:841	3%	840:841	3% MHBC/1% collagen	840:858	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	1	9	theme	printed	335:341	arg1	construct					343:351	the printed construct	331:351	the printed construct	331:351	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	4	10	theme	%	849:849	arg1	collagen					851:858	MHBC/1% collagen	843:858	3% MHBC/1% collagen	840:858	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	3	11	theme	rapid	521:525	arg1	transition					550:559	rapid thermo-induced sol-gel transition	521:559	rapid thermo-induced sol-gel transition	521:559	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	1	12	theme	construct	343:351	arg1	shape					296:300	the right shape	286:300	the right shape	286:300	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	12	theme	construct	343:351	arg1	properties					317:326	mechanical properties	306:326	mechanical properties	306:326	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	4	13	theme	formulation	827:837	arg1	printability					799:810	The printability	795:810	The printability of the optimal formulation (3% MHBC/1% collagen)	795:859	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	1	14	dep	In	104:105	arg1	situ					107:110	situ	107:110	situ	107:110	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	4	15	theme	optimal	819:825	arg1	formulation					827:837	the optimal formulation	815:837	the optimal formulation (3% MHBC/1% collagen)	815:859	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	4	16	dep	%	841:841	arg1	collagen					851:858	MHBC/1% collagen	843:858	3% MHBC/1% collagen	840:858	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	2	17	from	MHBC	447:450	arg1	study					481:485	this study	476:485	this study	476:485	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	4	18	theme	simple	945:950	arg1	manipulation					967:978	simple postprocessing manipulation	945:978	simple postprocessing manipulation	945:978	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	2	19	theme	composite	409:417	arg1	hydrogel					419:426	thermo/photo dual cure composite hydrogel	386:426	thermo/photo dual cure composite hydrogel	386:426	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	0	20	theme	cure	5:8	arg1	hydrogel					36:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	3	21	theme	M/C	488:490	arg1	hydrogel					502:509	M/C composite hydrogel	488:509	M/C composite hydrogel	488:509	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	0	22	dep	in	80:81	arg1	situ					83:86	situ	83:86	situ	83:86	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	0	23	theme	Dual	0:3	arg1	hydrogel					36:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	1	24	theme	gelation	257:264	arg1	kinetics					266:273	gelation kinetics	257:273	gelation kinetics	257:273	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	5	25	theme	potential	1064:1072	arg1	candidate					1074:1082	M/C composite hydrogel a potential candidate	1039:1082	M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting	1039:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	3	26	theme	MHBC	774:777	arg1	degree					764:769	the methacrylation degree	745:769	the methacrylation degree of MHBC and M/C ratios	745:792	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	1	27	theme	personalized	166:177	arg1	medicine					179:186	highly personalized medicine	159:186	highly personalized medicine	159:186	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	0	28	theme	thermal/photo	11:23	arg1	hydrogel					36:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	5	29	dep	in	1112:1113	arg1	situ					1115:1118	situ	1115:1118	situ	1115:1118	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	4	30	theme	mild	882:885	arg1	gelation					913:920	rapid gelation	907:920	rapid gelation of bioink	907:930	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	4	30	theme	mild	882:885	arg1	condition					896:904	its mild printing condition	878:904	its mild printing condition	878:904	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	3	31	theme	thermo-induced	527:540	arg1	transition					550:559	rapid thermo-induced sol-gel transition	521:559	rapid thermo-induced sol-gel transition	521:559	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	2	32	theme	thermo/photo	386:397	arg1	hydrogel					419:426	thermo/photo dual cure composite hydrogel	386:426	thermo/photo dual cure composite hydrogel	386:426	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	2	33	theme	dual	399:402	arg1	hydrogel					419:426	thermo/photo dual cure composite hydrogel	386:426	thermo/photo dual cure composite hydrogel	386:426	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	3	34	theme	composite	492:500	arg1	hydrogel					502:509	M/C composite hydrogel	488:509	M/C composite hydrogel	488:509	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	5	35	theme	composite	1043:1051	arg1	candidate					1074:1082	M/C composite hydrogel a potential candidate	1039:1082	M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting	1039:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	3	36	theme	sol-gel	542:548	arg1	transition					550:559	rapid thermo-induced sol-gel transition	521:559	rapid thermo-induced sol-gel transition	521:559	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	0	37	theme	composite	26:34	arg1	hydrogel					36:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel	0:43	Dual cure (thermal/photo) composite hydrogel derived from chitosan/collagen for in situ 3D bioprinting.					
33857517	3	38	theme	3D	656:657	arg1	culture					664:670	3D cell culture	656:670	3D cell culture	656:670	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	3	39	theme	tunable	578:584	arg1	properties					597:606	tunable mechanical properties	578:606	tunable mechanical properties	578:606	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	4	40	theme	printing	887:894	arg1	gelation					913:920	rapid gelation	907:920	rapid gelation of bioink	907:930	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	4	40	theme	printing	887:894	arg1	condition					896:904	its mild printing condition	878:904	its mild printing condition	878:904	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	5	41	theme	a	1062:1062	arg1	candidate					1074:1082	M/C composite hydrogel a potential candidate	1039:1082	M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting	1039:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	2	42	theme	cure	404:407	arg1	hydrogel					419:426	thermo/photo dual cure composite hydrogel	386:426	thermo/photo dual cure composite hydrogel	386:426	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	2	43	theme	facile	369:374	arg1	design					376:381	a facile design	367:381	a facile design of thermo/photo dual cure composite hydrogel	367:426	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	1	44	theme	right	290:294	arg1	shape					296:300	the right shape	286:300	the right shape	286:300	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	2	45	from	collagen	464:471	arg1	study					481:485	this study	476:485	this study	476:485	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	5	46	theme	desirable	986:994	arg1	printability					996:1007	desirable printability	986:1007	desirable printability	986:1007	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	1	47	theme	In	104:105	arg1	frontier					146:153	a new frontier	140:153	a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct	140:351	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	47	theme	In	104:105	arg1	technologies					124:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	4	48	theme	postprocessing	952:965	arg1	manipulation					967:978	simple postprocessing manipulation	945:978	simple postprocessing manipulation	945:978	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	3	49	theme	methacrylation	749:762	arg1	degree					764:769	the methacrylation degree	745:769	the methacrylation degree of MHBC and M/C ratios	745:792	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	4	50	theme	rapid	907:911	arg1	gelation					913:920	rapid gelation	907:920	rapid gelation of bioink	907:930	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	4	50	theme	rapid	907:911	arg1	condition					896:904	its mild printing condition	878:904	its mild printing condition	878:904	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	3	51	theme	proper	609:614	arg1	microstructure					616:629	proper microstructure	609:629	proper microstructure	609:629	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	3	52	theme	mechanical	586:595	arg1	properties					597:606	tunable mechanical properties	578:606	tunable mechanical properties	578:606	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	1	53	theme	3D	112:113	arg1	frontier					146:153	a new frontier	140:153	a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct	140:351	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	53	theme	3D	112:113	arg1	technologies					124:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	54	theme	suitable	204:211	arg1	bioink					213:218	suitable bioink	204:218	suitable bioink with rheology, biocompatibility, and gelation kinetics	204:273	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	3	55	theme	cell	659:662	arg1	culture					664:670	3D cell culture	656:670	3D cell culture	656:670	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
33857517	5	56	theme	hydrogel	1053:1060	arg1	candidate					1074:1082	M/C composite hydrogel a potential candidate	1039:1082	M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting	1039:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	1	57	theme	printing	115:122	arg1	frontier					146:153	a new frontier	140:153	a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct	140:351	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	1	57	theme	printing	115:122	arg1	technologies					124:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies	104:135	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	5	58	theme	3D	1120:1121	arg1	bioprinting					1123:1133	in situ 3D bioprinting	1112:1133	in situ 3D bioprinting	1112:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	5	59	theme	M/C	1039:1041	arg1	candidate					1074:1082	M/C composite hydrogel a potential candidate	1039:1082	M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting	1039:1133	Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.					
33857517	4	60	theme	bioink	925:930	arg1	gelation					913:920	rapid gelation	907:920	rapid gelation of bioink	907:930	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	4	60	theme	bioink	925:930	arg1	condition					896:904	its mild printing condition	878:904	its mild printing condition	878:904	The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation.					
33857517	1	61	theme	mechanical	306:315	arg1	properties					317:326	mechanical properties	306:326	mechanical properties	306:326	In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct.					
33857517	2	62	theme	hydrogel	419:426	arg1	design					376:381	a facile design	367:381	a facile design of thermo/photo dual cure composite hydrogel	367:426	To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study.					
33857517	3	63	theme	M/C	783:785	arg1	ratios					787:792	M/C ratios	783:792	M/C ratios	783:792	M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios.					
34346464	0	0	theme	sulphate	105:112	arg1	alleviation					82:92	their alleviation	76:92	their alleviation of dextran sulphate	76:112	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	0	0	theme	sulphate	105:112	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics of Gracilaria lemaneiformis oligosaccharides	0:70	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	5	1	from	loss	790:793	arg1	length					816:821	colon length	810:821	colon length	810:821	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	7	2	theme	fatty	1120:1124	arg1	acids					1126:1130	short-chain fatty acids	1108:1130	short-chain fatty acids	1108:1130	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	5	3	from	reductions	796:805	arg1	length					816:821	colon length	810:821	colon length	810:821	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	5	4	theme	sulphate	727:734	arg1	colitis					751:757	dextran sulphate sodium-induced colitis	719:757	dextran sulphate sodium-induced colitis	719:757	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	0	5	theme	dextran	97:103	arg1	sulphate					105:112	dextran sulphate	97:112	dextran sulphate	97:112	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	7	6	theme	acids	1126:1130	arg1	contents					1059:1066	the contents	1055:1066	the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model	1055:1157	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	1	7	theme	high	268:271	arg1	worldwide					283:291	a high incidence worldwide	266:291	a high incidence worldwide	266:291	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	2	8	theme	food-based	307:316	arg1	option					355:360	a good option	348:360	a good option for disease control	348:380	A functional food-based method to prevent UC would be a good option for disease control.					
34346464	2	8	theme	food-based	307:316	arg1	method					318:323	A functional food-based method	294:323	A functional food-based method to prevent UC	294:337	A functional food-based method to prevent UC would be a good option for disease control.					
34346464	0	9	from	metabolites	185:195	arg1	mice					200:203	mice	200:203	mice	200:203	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	4	10	from	GLOs	597:600	arg1	UC					605:606	UC	605:606	UC	605:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	6	11	theme	composition	884:894	arg1	analysis					896:903	Gut microbiota composition analysis	869:903	Gut microbiota composition analysis	869:903	Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes.					
34346464	7	12	theme	short-chain	1108:1118	arg1	acids					1126:1130	short-chain fatty acids	1108:1130	short-chain fatty acids	1108:1130	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	2	13	theme	functional	296:305	arg1	option					355:360	a good option	348:360	a good option for disease control	348:380	A functional food-based method to prevent UC would be a good option for disease control.					
34346464	2	13	theme	functional	296:305	arg1	method					318:323	A functional food-based method	294:323	A functional food-based method to prevent UC	294:337	A functional food-based method to prevent UC would be a good option for disease control.					
34346464	4	14	theme	GLOs	597:600	arg1	potential					584:592	the therapeutic potential	568:592	the therapeutic potential of GLOs in UC	568:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	4	14	theme	GLOs	597:600	arg1	mechanisms					635:644	their possible mechanisms	620:644	their possible mechanisms of action	620:654	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	5	15	theme	dextran	719:725	arg1	colitis					751:757	dextran sulphate sodium-induced colitis	719:757	dextran sulphate sodium-induced colitis	719:757	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	3	16	theme	in	493:494	arg1	fermentation					502:513	in vitro fermentation	493:513	in vitro fermentation assessed in our previous study	493:544	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	7	17	theme	microbial	1082:1090	arg1	metabolites					1092:1102	beneficial microbial metabolites	1071:1102	beneficial microbial metabolites	1071:1102	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	3	18	theme	previous	531:538	arg1	study					540:544	our previous study	527:544	our previous study	527:544	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	8	19	theme	UC	1245:1246	arg1	symptoms					1248:1255	UC symptoms	1245:1255	UC symptoms	1245:1255	In conclusion, GLOs could comprise a promising functional food strategy to alleviate UC symptoms.					
34346464	4	20	theme	action	649:654	arg1	potential					584:592	the therapeutic potential	568:592	the therapeutic potential of GLOs in UC	568:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	4	20	theme	action	649:654	arg1	mechanisms					635:644	their possible mechanisms	620:644	their possible mechanisms of action	620:654	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	4	21	from	potential	584:592	arg1	UC					605:606	UC	605:606	UC	605:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	6	22	theme	Gut	869:871	arg1	analysis					896:903	Gut microbiota composition analysis	869:903	Gut microbiota composition analysis	869:903	Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes.					
34346464	7	23	theme	mouse	1139:1143	arg1	model					1153:1157	the mouse colitis model	1135:1157	the mouse colitis model	1135:1157	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	1	24	theme	lifetime	243:250	arg1	disorder					252:259	a chronic lifetime disorder	233:259	a chronic lifetime disorder with a high incidence worldwide	233:291	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	1	24	theme	lifetime	243:250	arg1	colitis					217:223	Ulcerative colitis	206:223	Ulcerative colitis (UC)	206:228	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	7	25	from	contents	1059:1066	arg1	model					1153:1157	the mouse colitis model	1135:1157	the mouse colitis model	1135:1157	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	1	26	theme	Ulcerative	206:215	arg1	colitis					217:223	Ulcerative colitis	206:223	Ulcerative colitis (UC)	206:228	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	1	26	theme	Ulcerative	206:215	arg1	disorder					252:259	a chronic lifetime disorder	233:259	a chronic lifetime disorder with a high incidence worldwide	233:291	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	1	26	theme	Ulcerative	206:215	arg1	UC					226:227	UC	226:227	UC	226:227	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	3	27	theme	lemaneiformis	386:398	arg1	GLOs					418:421	GLOs	418:421	GLOs	418:421	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	3	27	theme	lemaneiformis	386:398	arg1	oligosaccharides					400:415	G. lemaneiformis oligosaccharides	383:415	G. lemaneiformis oligosaccharides (GLOs)	383:422	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	4	28	from	UC	605:606	arg1	potential					584:592	the therapeutic potential	568:592	the therapeutic potential of GLOs in UC	568:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	4	28	from	UC	605:606	arg1	mechanisms					635:644	their possible mechanisms	620:644	their possible mechanisms of action	620:654	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	6	29	theme	microbiota	873:882	arg1	analysis					896:903	Gut microbiota composition analysis	869:903	Gut microbiota composition analysis	869:903	Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes.					
34346464	5	30	theme	colon	855:859	arg1	damage					861:866	colon damage	855:866	colon damage	855:866	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	0	31	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics of Gracilaria lemaneiformis oligosaccharides	0:70	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	7	32	theme	beneficial	1071:1080	arg1	metabolites					1092:1102	beneficial microbial metabolites	1071:1102	beneficial microbial metabolites	1071:1102	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	4	33	from	mechanisms	635:644	arg1	UC					605:606	UC	605:606	UC	605:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	3	34	theme	potent	436:441	arg1	benefits					443:450	potent benefits	436:450	potent benefits for the gastrointestinal tract	436:481	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	0	35	theme	Gracilaria	30:39	arg1	oligosaccharides					55:70	Gracilaria lemaneiformis oligosaccharides	30:70	Gracilaria lemaneiformis oligosaccharides	30:70	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	0	36	theme	gut	155:157	arg1	microbiota					159:168	the gut microbiota	151:168	the gut microbiota	151:168	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	8	37	theme	promising	1197:1205	arg1	strategy					1223:1230	a promising functional food strategy	1195:1230	a promising functional food strategy to alleviate UC symptoms	1195:1255	In conclusion, GLOs could comprise a promising functional food strategy to alleviate UC symptoms.					
34346464	3	38	theme	G.	383:384	arg1	GLOs					418:421	GLOs	418:421	GLOs	418:421	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	3	38	theme	G.	383:384	arg1	oligosaccharides					400:415	G. lemaneiformis oligosaccharides	383:415	G. lemaneiformis oligosaccharides (GLOs)	383:422	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	4	39	theme	therapeutic	572:582	arg1	potential					584:592	the therapeutic potential	568:592	the therapeutic potential of GLOs in UC	568:606	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	3	40	dep	in	493:494	arg1	vitro					496:500	vitro	496:500	vitro	496:500	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	0	41	theme	oligosaccharides	55:70	arg1	alleviation					82:92	their alleviation	76:92	their alleviation of dextran sulphate	76:112	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	0	41	theme	oligosaccharides	55:70	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics of Gracilaria lemaneiformis oligosaccharides	0:70	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	5	42	theme	inflammatory	824:835	arg1	infiltration					837:848	inflammatory infiltration	824:848	inflammatory infiltration	824:848	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	7	43	theme	colitis	1145:1151	arg1	model					1153:1157	the mouse colitis model	1135:1157	the mouse colitis model	1135:1157	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	6	44	theme	Bacteroidetes	976:988	arg1	composition					961:971	the composition	957:971	the composition of Bacteroidetes	957:988	Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes.					
34346464	3	45	theme	gastrointestinal	460:475	arg1	tract					477:481	the gastrointestinal tract	456:481	the gastrointestinal tract	456:481	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	5	46	theme	weight	783:788	arg1	loss					790:793	weight loss	783:793	weight loss	783:793	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	6	47	theme	Firmicutes	1016:1025	arg1	level					1007:1011	the level	1003:1011	the level of Firmicutes	1003:1025	Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes.					
34346464	5	48	theme	colitis	751:757	arg1	severity					707:714	the severity	703:714	the severity of dextran sulphate sodium-induced colitis	703:757	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	5	49	from	infiltration	837:848	arg1	length					816:821	colon length	810:821	colon length	810:821	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	2	50	theme	good	350:353	arg1	method					318:323	A functional food-based method	294:323	A functional food-based method to prevent UC	294:337	A functional food-based method to prevent UC would be a good option for disease control.					
34346464	2	50	theme	good	350:353	arg1	option					355:360	a good option	348:360	a good option for disease control	348:380	A functional food-based method to prevent UC would be a good option for disease control.					
34346464	8	51	theme	functional	1207:1216	arg1	strategy					1223:1230	a promising functional food strategy	1195:1230	a promising functional food strategy to alleviate UC symptoms	1195:1255	In conclusion, GLOs could comprise a promising functional food strategy to alleviate UC symptoms.					
34346464	6	52	theme	phylum	924:929	arg1	level					931:935	the phylum level	920:935	the phylum level	920:935	Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes.					
34346464	0	53	theme	intestinal	174:183	arg1	metabolites					185:195	intestinal metabolites	174:195	intestinal metabolites in mice	174:203	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	7	54	theme	metabolites	1092:1102	arg1	contents					1059:1066	the contents	1055:1066	the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model	1055:1157	Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model.					
34346464	5	55	theme	GLOs	679:682	arg1	administration					661:674	The administration	657:674	The administration of GLOs	657:682	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	5	55	theme	GLOs	679:682	arg1	able					688:691	able	688:691	able	688:691	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	3	56	contain	have	431:434	arg1	GLOs					418:421	GLOs	418:421	GLOs	418:421	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	3	56	contain	have	431:434	arg1	oligosaccharides					400:415	G. lemaneiformis oligosaccharides	383:415	G. lemaneiformis oligosaccharides (GLOs)	383:422	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	3	56	contain	have	431:434	arg2	benefits					443:450	potent benefits	436:450	potent benefits for the gastrointestinal tract	436:481	G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study.					
34346464	0	57	dep	Gracilaria	30:39	arg1	lemaneiformis					41:53	lemaneiformis	41:53	lemaneiformis	41:53	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	1	58	theme	chronic	235:241	arg1	disorder					252:259	a chronic lifetime disorder	233:259	a chronic lifetime disorder with a high incidence worldwide	233:291	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	1	58	theme	chronic	235:241	arg1	colitis					217:223	Ulcerative colitis	206:223	Ulcerative colitis (UC)	206:228	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	1	59	with	disorder	252:259	arg1	worldwide					283:291	a high incidence worldwide	266:291	a high incidence worldwide	266:291	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	8	60	theme	food	1218:1221	arg1	strategy					1223:1230	a promising functional food strategy	1195:1230	a promising functional food strategy to alleviate UC symptoms	1195:1255	In conclusion, GLOs could comprise a promising functional food strategy to alleviate UC symptoms.					
34346464	0	61	from	microbiota	159:168	arg1	mice					200:203	mice	200:203	mice	200:203	Structural characteristics of Gracilaria lemaneiformis oligosaccharides and their alleviation of dextran sulphate sodium-induced colitis by modulating the gut microbiota and intestinal metabolites in mice.					
34346464	5	62	theme	sodium-induced	736:749	arg1	colitis					751:757	dextran sulphate sodium-induced colitis	719:757	dextran sulphate sodium-induced colitis	719:757	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	4	63	theme	possible	626:633	arg1	mechanisms					635:644	their possible mechanisms	620:644	their possible mechanisms of action	620:654	This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action.					
34346464	5	64	from	damage	861:866	arg1	length					816:821	colon length	810:821	colon length	810:821	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	1	65	theme	incidence	273:281	arg1	worldwide					283:291	a high incidence worldwide	266:291	a high incidence worldwide	266:291	Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide.					
34346464	5	66	theme	colon	810:814	arg1	length					816:821	colon length	810:821	colon length	810:821	The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage.					
34346464	2	67	theme	disease	366:372	arg1	control					374:380	disease control	366:380	disease control	366:380	A functional food-based method to prevent UC would be a good option for disease control.					
34044032	6	0	theme	Adsorption	855:864	arg1	energy					866:871	Adsorption energy	855:871	Adsorption energy	855:871	Adsorption energy was 12.1 kJ mol-1 indicating that the adsorption process was chemical.					
34044032	8	1	theme	UO22+	1071:1075	arg1	ions					1077:1080	UO22+ ions	1071:1080	UO22+ ions removal from aqueous solution	1071:1110	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	1	2	theme	green	138:142	arg1	approach					163:170	a green and cost-effective approach	136:170	a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution	136:285	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	4	3	theme	adsorptive	549:558	arg1	processes					568:576	The adsorptive removal processes	545:576	The adsorptive removal processes of UO22+ ions from aqueous solution	545:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	3	theme	adsorptive	549:558	arg1	fit					725:727	fit	725:727	fit	725:727	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	8	4	theme	aqueous	1095:1101	arg1	solution					1103:1110	aqueous solution	1095:1110	aqueous solution	1095:1110	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	9	5	theme	Ch-Sep	1263:1268	arg1	beads					1280:1284	Ch-Sep composite beads	1263:1284	Ch-Sep composite beads	1263:1284	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	10	6	theme	beads	1428:1432	arg1	potential					1376:1384	potential	1376:1384	potential	1376:1384	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	10	6	theme	beads	1428:1432	arg1	effectiveness					1358:1370	effectiveness	1358:1370	effectiveness	1358:1370	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	5	7	theme	Langmuir	830:837	arg1	model					848:852	Langmuir isotherm model	830:852	Langmuir isotherm model	830:852	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	3	8	theme	ions	473:476	arg1	removal					456:462	the removal	452:462	the removal of UO22+ ions in aqueous solution by batch experimental conditions	452:529	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	4	9	from	processes	568:576	arg1	solution					605:612	aqueous solution	597:612	aqueous solution	597:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	9	10	theme	composite	1270:1278	arg1	beads					1280:1284	Ch-Sep composite beads	1263:1284	Ch-Sep composite beads	1263:1284	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	10	11	theme	composite	1418:1426	arg1	beads					1428:1432	the newly synthesized Ch-Sep composite beads	1389:1432	the newly synthesized Ch-Sep composite beads	1389:1432	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	3	12	from	ions	473:476	arg1	solution					489:496	aqueous solution	481:496	aqueous solution	481:496	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	3	13	theme	batch	501:505	arg1	conditions					520:529	batch experimental conditions	501:529	batch experimental conditions	501:529	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	8	14	dep	ions	1077:1080	arg1	removal					1082:1088	removal	1082:1088	UO22+ ions removal from aqueous solution	1071:1110	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	5	15	from	°C	822:823	arg1	kg-1					811:814	0.220 mol kg-1	801:814	0.220 mol kg-1	801:814	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	5	15	from	°C	822:823	arg1	capacity					788:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	3	16	theme	experimental	507:518	arg1	conditions					520:529	batch experimental conditions	501:529	batch experimental conditions	501:529	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	1	17	theme	UO22+	256:260	arg1	ions					262:265	UO22+ ions	256:265	UO22+ ions	256:265	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	10	18	theme	UO22+	1453:1457	arg1	ions					1459:1462	UO22+ ions	1453:1462	UO22+ ions	1453:1462	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	9	19	theme	FT-IR	1306:1310	arg1	analysis					1320:1327	FT-IR and SEM analysis	1306:1327	FT-IR and SEM analysis	1306:1327	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	8	20	theme	possible	1155:1162	arg1	behavior					1187:1194	spontaneous, endothermic and possible at higher temperatures behavior	1126:1194	spontaneous, endothermic and possible at higher temperatures behavior of adsorption process	1126:1216	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	1	21	theme	cost-effective	148:161	arg1	approach					163:170	a green and cost-effective approach	136:170	a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution	136:285	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	4	22	theme	removal	560:566	arg1	processes					568:576	The adsorptive removal processes	545:576	The adsorptive removal processes of UO22+ ions from aqueous solution	545:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	22	theme	removal	560:566	arg1	fit					725:727	fit	725:727	fit	725:727	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	5	23	theme	isotherm	839:846	arg1	model					848:852	Langmuir isotherm model	830:852	Langmuir isotherm model	830:852	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	10	24	theme	ions	1459:1462	arg1	removal					1442:1448	the removal	1438:1448	the removal of UO22+ ions	1438:1462	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	4	25	theme	Langmuir	736:743	arg1	model					745:749	the Langmuir model	732:749	the Langmuir model	732:749	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	26	dep	=	755:755	arg1	0.971					757:761	0.971	757:761	0.971	757:761	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	0	27	theme	chitosan-based	2:15	arg1	composite					17:25	A chitosan-based composite	0:25	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.	0:106	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	10	28	theme	synthesized	1399:1409	arg1	beads					1428:1432	the newly synthesized Ch-Sep composite beads	1389:1432	the newly synthesized Ch-Sep composite beads	1389:1432	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	6	29	theme	adsorption	911:920	arg1	chemical					934:941	chemical	934:941	chemical	934:941	Adsorption energy was 12.1 kJ mol-1 indicating that the adsorption process was chemical.					
34044032	6	29	theme	adsorption	911:920	arg1	process					922:928	the adsorption process	907:928	the adsorption process	907:928	Adsorption energy was 12.1 kJ mol-1 indicating that the adsorption process was chemical.					
34044032	8	30	theme	endothermic	1139:1149	arg1	behavior					1187:1194	spontaneous, endothermic and possible at higher temperatures behavior	1126:1194	spontaneous, endothermic and possible at higher temperatures behavior of adsorption process	1126:1216	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	1	31	theme	ions	262:265	arg1	removal					245:251	removal	245:251	removal of UO22+ ions in aqueous solution	245:285	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	8	32	theme	process	1210:1216	arg1	behavior					1187:1194	spontaneous, endothermic and possible at higher temperatures behavior	1126:1194	spontaneous, endothermic and possible at higher temperatures behavior of adsorption process	1126:1216	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	3	33	from	removal	456:462	arg1	solution					489:496	aqueous solution	481:496	aqueous solution	481:496	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	2	34	theme	Ch-Sep	292:297	arg1	beads					327:331	a beads	325:331	a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH)	325:417	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	34	theme	Ch-Sep	292:297	arg1	composite					299:307	The Ch-Sep composite	288:307	The Ch-Sep composite	288:307	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	10	35	theme	Ch-Sep	1411:1416	arg1	beads					1428:1432	the newly synthesized Ch-Sep composite beads	1389:1432	the newly synthesized Ch-Sep composite beads	1389:1432	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	2	36	theme	cross-linking	348:360	arg1	epichlorohydrin					397:411	epichlorohydrin	397:411	epichlorohydrin (ECH)	397:417	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	36	theme	cross-linking	348:360	arg1	tripolyphosphate					370:385	tripolyphosphate	370:385	tripolyphosphate (TPP)	370:391	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	36	theme	cross-linking	348:360	arg1	agents					362:367	two cross-linking agents	344:367	two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH)	344:417	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	4	37	dep	Freundlich	642:651	arg1	models					687:692	isotherm models	678:692	isotherm models	678:692	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	7	38	theme	particle	1011:1018	arg1	models					1030:1035	intra particle diffusion models	1005:1035	intra particle diffusion models	1005:1035	The adsorption kinetics followed the pseudo second order and intra particle diffusion models.					
34044032	3	39	theme	adsorption	426:435	arg1	properties					437:446	Their adsorption properties	420:446	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions	420:529	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	5	40	theme	maximum	769:775	arg1	kg-1					811:814	0.220 mol kg-1	801:814	0.220 mol kg-1	801:814	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	5	40	theme	maximum	769:775	arg1	capacity					788:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	1	41	theme	aqueous	270:276	arg1	solution					278:285	aqueous solution	270:285	aqueous solution	270:285	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	7	42	theme	diffusion	1020:1028	arg1	models					1030:1035	intra particle diffusion models	1005:1035	intra particle diffusion models	1005:1035	The adsorption kinetics followed the pseudo second order and intra particle diffusion models.					
34044032	8	43	from	solution	1103:1110	arg1	removal					1082:1088	removal	1082:1088	UO22+ ions removal from aqueous solution	1071:1110	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	4	44	theme	isotherm	678:685	arg1	models					687:692	isotherm models	678:692	isotherm models	678:692	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	45	from	solution	605:612	arg1	processes					568:576	The adsorptive removal processes	545:576	The adsorptive removal processes of UO22+ ions from aqueous solution	545:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	45	from	solution	605:612	arg1	ions					587:590	UO22+ ions	581:590	UO22+ ions from aqueous solution	581:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	45	from	solution	605:612	arg1	fit					725:727	fit	725:727	fit	725:727	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	46	theme	aqueous	597:603	arg1	solution					605:612	aqueous solution	597:612	aqueous solution	597:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	3	47	theme	UO22+	467:471	arg1	ions					473:476	UO22+ ions	467:476	UO22+ ions in aqueous solution	467:496	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	8	48	theme	thermodynamics	1042:1055	arg1	parameters					1057:1066	The thermodynamics parameters	1038:1066	The thermodynamics parameters of UO22+ ions removal from aqueous solution	1038:1110	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	10	49	dep	effectiveness	1358:1370	arg1	the					1354:1356	the	1354:1356	the	1354:1356	These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions.					
34044032	4	50	dep	evaluated	619:627	arg1	R2					752:753	R2	752:753	R2 = 0.971	752:761	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	9	51	theme	SEM	1316:1318	arg1	analysis					1320:1327	FT-IR and SEM analysis	1306:1327	FT-IR and SEM analysis	1306:1327	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	5	52	theme	mol	807:809	arg1	kg-1					811:814	0.220 mol kg-1	801:814	0.220 mol kg-1	801:814	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	5	52	theme	mol	807:809	arg1	capacity					788:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	1	53	from	removal	245:251	arg1	solution					278:285	aqueous solution	270:285	aqueous solution	270:285	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	8	54	theme	spontaneous	1126:1136	arg1	behavior					1187:1194	spontaneous, endothermic and possible at higher temperatures behavior	1126:1194	spontaneous, endothermic and possible at higher temperatures behavior of adsorption process	1126:1216	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	0	55	theme	ions	52:55	arg1	adsorption					31:40	adsorption	31:40	adsorption of uranyl ions	31:55	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	6	56	theme	kJ	882:883	arg1	mol-1					885:889	12.1 kJ mol-1	877:889	12.1 kJ mol-1	877:889	Adsorption energy was 12.1 kJ mol-1 indicating that the adsorption process was chemical.					
34044032	7	57	theme	second	988:993	arg1	order					995:999	the pseudo second order	977:999	the pseudo second order	977:999	The adsorption kinetics followed the pseudo second order and intra particle diffusion models.					
34044032	8	58	from	temperatures	1174:1185	arg1	possible					1155:1162	possible	1155:1162	possible	1155:1162	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	7	59	theme	intra	1005:1009	arg1	models					1030:1035	intra particle diffusion models	1005:1035	intra particle diffusion models	1005:1035	The adsorption kinetics followed the pseudo second order and intra particle diffusion models.					
34044032	0	60	theme	uranyl	45:50	arg1	ions					52:55	uranyl ions	45:55	uranyl ions	45:55	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	9	61	theme	adsorption	1223:1232	arg1	mechanism					1234:1242	The adsorption mechanism	1219:1242	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads	1219:1284	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	8	62	theme	ions	1077:1080	arg1	parameters					1057:1066	The thermodynamics parameters	1038:1066	The thermodynamics parameters of UO22+ ions removal from aqueous solution	1038:1110	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	1	63	attach	removal	245:251	arg2	ions					262:265	UO22+ ions	256:265	UO22+ ions	256:265	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	1	63	attach	removal	245:251	arg3	solution					278:285	aqueous solution	270:285	aqueous solution	270:285	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	2	64	dep	agents	362:367	arg1	ECH					414:416	ECH	414:416	ECH	414:416	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	64	dep	agents	362:367	arg1	TPP					388:390	TPP	388:390	TPP	388:390	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	64	dep	agents	362:367	arg1	epichlorohydrin					397:411	epichlorohydrin	397:411	epichlorohydrin (ECH)	397:417	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	64	dep	agents	362:367	arg1	tripolyphosphate					370:385	tripolyphosphate	370:385	tripolyphosphate (TPP)	370:391	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	2	64	dep	agents	362:367	arg1	agents					362:367	two cross-linking agents	344:367	two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH)	344:417	The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH).					
34044032	7	65	theme	adsorption	948:957	arg1	kinetics					959:966	The adsorption kinetics	944:966	The adsorption kinetics	944:966	The adsorption kinetics followed the pseudo second order and intra particle diffusion models.					
34044032	1	66	theme	present	112:118	arg1	paper					120:124	The present paper	108:124	The present paper	108:124	The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution.					
34044032	4	67	theme	UO22+	581:585	arg1	ions					587:590	UO22+ ions	581:590	UO22+ ions from aqueous solution	581:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	7	68	theme	pseudo	981:986	arg1	order					995:999	the pseudo second order	977:999	the pseudo second order	977:999	The adsorption kinetics followed the pseudo second order and intra particle diffusion models.					
34044032	0	69	dep	composite	17:25	arg1	kinetics					79:86	kinetics	79:86	kinetics	79:86	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	0	69	dep	composite	17:25	arg1	isothems					69:76	isothems	69:76	isothems	69:76	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	0	69	dep	composite	17:25	arg1	mechanism					58:66	mechanism	58:66	mechanism	58:66	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	0	69	dep	composite	17:25	arg1	thermodynamics					92:105	thermodynamics	92:105	thermodynamics	92:105	A chitosan-based composite for adsorption of uranyl ions; mechanism, isothems, kinetics and thermodynamics.					
34044032	8	70	theme	higher	1167:1172	arg1	temperatures					1174:1185	higher temperatures	1167:1185	higher temperatures	1167:1185	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	9	71	theme	UO22+	1247:1251	arg1	ions					1253:1256	UO22+ ions	1247:1256	UO22+ ions	1247:1256	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	5	72	from	model	848:852	arg1	°C					822:823	25 °C	819:823	25 °C from Langmuir isotherm model	819:852	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	5	73	theme	adsorption	777:786	arg1	kg-1					811:814	0.220 mol kg-1	801:814	0.220 mol kg-1	801:814	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	5	73	theme	adsorption	777:786	arg1	capacity					788:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity	765:795	The maximum adsorption capacity was 0.220 mol kg-1 at 25 °C from Langmuir isotherm model.					
34044032	8	74	theme	adsorption	1199:1208	arg1	process					1210:1216	adsorption process	1199:1216	adsorption process	1199:1216	The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process.					
34044032	9	75	theme	ions	1253:1256	arg1	mechanism					1234:1242	The adsorption mechanism	1219:1242	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads	1219:1284	The adsorption mechanism of UO22+ ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis.					
34044032	4	76	theme	=	755:755	arg1	R2					752:753	R2	752:753	R2 = 0.971	752:761	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	3	77	theme	aqueous	481:487	arg1	solution					489:496	aqueous solution	481:496	aqueous solution	481:496	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	3	78	from	solution	489:496	arg1	removal					456:462	the removal	452:462	the removal of UO22+ ions in aqueous solution by batch experimental conditions	452:529	Their adsorption properties for the removal of UO22+ ions in aqueous solution by batch experimental conditions were studied.					
34044032	4	79	theme	ions	587:590	arg1	processes					568:576	The adsorptive removal processes	545:576	The adsorptive removal processes of UO22+ ions from aqueous solution	545:612	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
34044032	4	79	theme	ions	587:590	arg1	fit					725:727	fit	725:727	fit	725:727	The adsorptive removal processes of UO22+ ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R2 = 0.971).					
33058971	6	0	theme	complex	864:870	arg1	system					911:916	a promising delivery system	890:916	a promising delivery system for hydrophobic bioactive compounds	890:952	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	6	0	theme	complex	864:870	arg1	nanoparticles					872:884	SPI-CNC complex nanoparticles	856:884	SPI-CNC complex nanoparticles	856:884	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	6	1	theme	SPI-CNC	856:862	arg1	system					911:916	a promising delivery system	890:916	a promising delivery system for hydrophobic bioactive compounds	890:952	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	6	1	theme	SPI-CNC	856:862	arg1	nanoparticles					872:884	SPI-CNC complex nanoparticles	856:884	SPI-CNC complex nanoparticles	856:884	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	2	2	theme	mass	318:321	arg1	ratio					323:327	the SPI-to-CNC mass ratio	303:327	the SPI-to-CNC mass ratio	303:327	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	4	3	from	temperatures	625:636	arg1	nanosystem					562:571	The nanosystem	558:571	The nanosystem	558:571	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	3	from	temperatures	625:636	arg1	stable					588:593	stable	588:593	stable	588:593	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	1	4	theme	cellulose	239:247	arg1	matrices					279:286	polymer matrices	271:286	polymer matrices	271:286	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	4	theme	cellulose	239:247	arg1	isolate					221:227	soy protein isolate	209:227	soy protein isolate (SPI)	209:233	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	4	theme	cellulose	239:247	arg1	CNC					263:265	CNC	263:265	CNC	263:265	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	4	theme	cellulose	239:247	arg1	nanocrystals					249:260	cellulose nanocrystals	239:260	cellulose nanocrystals (CNC)	239:266	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	3	5	theme	small	473:477	arg1	197.7 ± 0.2 nm					485:498	197.7 ± 0.2 nm	485:498	197.7 ± 0.2 nm	485:498	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	5	theme	small	473:477	arg1	size					479:482	a relatively small size	460:482	a relatively small size (197.7 ± 0.2 nm)	460:499	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	6	theme	low	505:507	arg1	index					524:528	low polydispersity index	505:528	low polydispersity index (0.14)	505:535	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	6	theme	low	505:507	arg1	0.14					531:534	0.14	531:534	0.14	531:534	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	1	7	theme	curcumin	176:183	arg1	delivery					191:198	curcumin (Cur) delivery	176:198	curcumin (Cur) delivery	176:198	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	5	8	theme	complex	706:712	arg1	nanoparticles					714:726	the complex nanoparticles	702:726	the complex nanoparticles	702:726	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	3	9	theme	polydispersity	509:522	arg1	index					524:528	low polydispersity index	505:528	low polydispersity index (0.14)	505:535	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	9	theme	polydispersity	509:522	arg1	0.14					531:534	0.14	531:534	0.14	531:534	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	2	10	theme	physical	358:365	arg1	properties					367:376	physical properties	358:376	physical properties	358:376	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	5	11	theme	high	740:743	arg1	efficiency					759:768	a high encapsulation efficiency	738:768	a high encapsulation efficiency (88.3%)	738:776	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	5	11	theme	high	740:743	arg1	%					775:775	88.3%	771:775	88.3%	771:775	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	4	12	from	values	611:616	arg1	nanosystem					562:571	The nanosystem	558:571	The nanosystem	558:571	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	12	from	values	611:616	arg1	stable					588:593	stable	588:593	stable	588:593	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	5	13	theme	encapsulation	745:757	arg1	efficiency					759:768	a high encapsulation efficiency	738:768	a high encapsulation efficiency (88.3%)	738:776	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	5	13	theme	encapsulation	745:757	arg1	%					775:775	88.3%	771:775	88.3%	771:775	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	6	14	theme	bioactive	934:942	arg1	compounds					944:952	hydrophobic bioactive compounds	922:952	hydrophobic bioactive compounds	922:952	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	5	15	theme	simulated	807:815	arg1	digestion					834:842	simulated gastrointestinal digestion	807:842	simulated gastrointestinal digestion	807:842	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	0	16	theme	protein	19:25	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of soy protein	0:25	Fabrication of soy protein isolate/cellulose nanocrystal composite nanoparticles for curcumin delivery.					
33058971	6	17	theme	hydrophobic	922:932	arg1	compounds					944:952	hydrophobic bioactive compounds	922:952	hydrophobic bioactive compounds	922:952	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	4	18	from	concentrations	659:672	arg1	nanosystem					562:571	The nanosystem	558:571	The nanosystem	558:571	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	18	from	concentrations	659:672	arg1	stable					588:593	stable	588:593	stable	588:593	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	2	19	theme	complex	393:399	arg1	nanoparticles					401:413	the SPI-CNC complex nanoparticles	381:413	the SPI-CNC complex nanoparticles	381:413	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	2	20	theme	nanoparticles	401:413	arg1	stability					344:352	stability	344:352	stability	344:352	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	2	20	theme	nanoparticles	401:413	arg1	properties					367:376	physical properties	358:376	physical properties	358:376	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	2	21	theme	SPI-CNC	385:391	arg1	nanoparticles					401:413	the SPI-CNC complex nanoparticles	381:413	the SPI-CNC complex nanoparticles	381:413	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	0	22	theme	nanocrystal	45:55	arg1	nanoparticles					67:79	nanocrystal composite nanoparticles	45:79	nanocrystal composite nanoparticles for curcumin delivery	45:101	Fabrication of soy protein isolate/cellulose nanocrystal composite nanoparticles for curcumin delivery.					
33058971	4	23	theme	pH	608:609	arg1	3-9					619:621	3-9	619:621	3-9	619:621	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	23	theme	pH	608:609	arg1	values					611:616	different pH values	598:616	different pH values (3-9)	598:622	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	24	theme	different	598:606	arg1	3-9					619:621	3-9	619:621	3-9	619:621	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	24	theme	different	598:606	arg1	values					611:616	different pH values	598:616	different pH values (3-9)	598:622	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	3	25	theme	SPI-CNC	426:432	arg1	nanoparticles					442:454	SPI-CNC complex nanoparticles	426:454	SPI-CNC complex nanoparticles	426:454	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	5	26	theme	sustained	782:790	arg1	release					792:798	sustained release	782:798	sustained release	782:798	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	1	27	theme	polymer	271:277	arg1	matrices					279:286	polymer matrices	271:286	polymer matrices	271:286	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	27	theme	polymer	271:277	arg1	isolate					221:227	soy protein isolate	209:227	soy protein isolate (SPI)	209:233	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	27	theme	polymer	271:277	arg1	nanocrystals					249:260	cellulose nanocrystals	239:260	cellulose nanocrystals (CNC)	239:266	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	3	28	contain	had	456:458	arg2	size					479:482	a relatively small size	460:482	a relatively small size (197.7 ± 0.2 nm)	460:499	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	28	contain	had	456:458	arg2	197.7 ± 0.2 nm					485:498	197.7 ± 0.2 nm	485:498	197.7 ± 0.2 nm	485:498	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	28	contain	had	456:458	arg1	nanoparticles					442:454	SPI-CNC complex nanoparticles	426:454	SPI-CNC complex nanoparticles	426:454	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	28	contain	had	456:458	arg2	0.14					531:534	0.14	531:534	0.14	531:534	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	3	28	contain	had	456:458	arg2	index					524:528	low polydispersity index	505:528	low polydispersity index (0.14)	505:535	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	1	29	theme	soy	209:211	arg1	matrices					279:286	polymer matrices	271:286	polymer matrices	271:286	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	29	theme	soy	209:211	arg1	SPI					230:232	SPI	230:232	SPI	230:232	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	29	theme	soy	209:211	arg1	isolate					221:227	soy protein isolate	209:227	soy protein isolate (SPI)	209:233	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	29	theme	soy	209:211	arg1	nanocrystals					249:260	cellulose nanocrystals	239:260	cellulose nanocrystals (CNC)	239:266	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	6	30	theme	delivery	902:909	arg1	system					911:916	a promising delivery system	890:916	a promising delivery system for hydrophobic bioactive compounds	890:952	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	6	30	theme	delivery	902:909	arg1	nanoparticles					872:884	SPI-CNC complex nanoparticles	856:884	SPI-CNC complex nanoparticles	856:884	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	3	31	theme	6:1	542:544	arg1	ratio					551:555	a 6:1 mass ratio	540:555	a 6:1 mass ratio	540:555	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	2	32	theme	SPI-to-CNC	307:316	arg1	ratio					323:327	the SPI-to-CNC mass ratio	303:327	the SPI-to-CNC mass ratio	303:327	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	0	33	theme	composite	57:65	arg1	nanoparticles					67:79	nanocrystal composite nanoparticles	45:79	nanocrystal composite nanoparticles for curcumin delivery	45:101	Fabrication of soy protein isolate/cellulose nanocrystal composite nanoparticles for curcumin delivery.					
33058971	3	34	theme	mass	546:549	arg1	ratio					551:555	a 6:1 mass ratio	540:555	a 6:1 mass ratio	540:555	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	1	35	theme	novel	132:136	arg1	nanoparticles					146:158	novel complex nanoparticles	132:158	novel complex nanoparticles as carriers for curcumin (Cur) delivery	132:198	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	36	theme	protein	213:219	arg1	matrices					279:286	polymer matrices	271:286	polymer matrices	271:286	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	36	theme	protein	213:219	arg1	SPI					230:232	SPI	230:232	SPI	230:232	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	36	theme	protein	213:219	arg1	isolate					221:227	soy protein isolate	209:227	soy protein isolate (SPI)	209:233	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	1	36	theme	protein	213:219	arg1	nanocrystals					249:260	cellulose nanocrystals	239:260	cellulose nanocrystals (CNC)	239:266	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	2	37	dep	stability	344:352	arg1	the					340:342	the	340:342	the	340:342	We found that the SPI-to-CNC mass ratio influenced the stability and physical properties of the SPI-CNC complex nanoparticles.					
33058971	1	38	theme	complex	138:144	arg1	nanoparticles					146:158	novel complex nanoparticles	132:158	novel complex nanoparticles as carriers for curcumin (Cur) delivery	132:198	In this study, we developed novel complex nanoparticles as carriers for curcumin (Cur) delivery by using soy protein isolate (SPI) and cellulose nanocrystals (CNC) as polymer matrices.					
33058971	4	39	theme	salt	654:657	arg1	0-40 mmol/L					675:685	0-40 mmol/L	675:685	0-40 mmol/L	675:685	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	4	39	theme	salt	654:657	arg1	concentrations					659:672	salt concentrations	654:672	salt concentrations (0-40 mmol/L)	654:686	The nanosystem was relatively stable at different pH values (3-9), temperatures (30-90 °C), and salt concentrations (0-40 mmol/L).					
33058971	0	40	theme	curcumin	85:92	arg1	delivery					94:101	curcumin delivery	85:101	curcumin delivery	85:101	Fabrication of soy protein isolate/cellulose nanocrystal composite nanoparticles for curcumin delivery.					
33058971	3	41	theme	complex	434:440	arg1	nanoparticles					442:454	SPI-CNC complex nanoparticles	426:454	SPI-CNC complex nanoparticles	426:454	Moreover, SPI-CNC complex nanoparticles had a relatively small size (197.7 ± 0.2 nm) and low polydispersity index (0.14) at a 6:1 mass ratio.					
33058971	5	42	theme	gastrointestinal	817:832	arg1	digestion					834:842	simulated gastrointestinal digestion	807:842	simulated gastrointestinal digestion	807:842	Furthermore, the complex nanoparticles exhibited a high encapsulation efficiency (88.3%) and sustained release during simulated gastrointestinal digestion.					
33058971	0	43	theme	soy	15:17	arg1	protein					19:25	soy protein	15:25	soy protein	15:25	Fabrication of soy protein isolate/cellulose nanocrystal composite nanoparticles for curcumin delivery.					
33058971	6	44	theme	promising	892:900	arg1	system					911:916	a promising delivery system	890:916	a promising delivery system for hydrophobic bioactive compounds	890:952	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
33058971	6	44	theme	promising	892:900	arg1	nanoparticles					872:884	SPI-CNC complex nanoparticles	856:884	SPI-CNC complex nanoparticles	856:884	Therefore, SPI-CNC complex nanoparticles are a promising delivery system for hydrophobic bioactive compounds.					
32603085	6	0	theme	cellulose	1028:1036	arg1	backbone					1038:1045	the cellulose backbone	1024:1045	the cellulose backbone of wood sawdust	1024:1061	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	6	1	theme	flow	929:932	arg1	characteristics					934:948	Such water flow characteristics	918:948	Such water flow characteristics	918:948	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	6	2	theme	wood	1050:1053	arg1	sawdust					1055:1061	wood sawdust	1050:1061	wood sawdust	1050:1061	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	6	3	theme	pharmaceutical	978:991	arg1	pollutants					993:1002	pharmaceutical pollutants	978:1002	pharmaceutical pollutants	978:1002	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	0	4	from	Polymerized	15:25	arg1	Channels					44:51	Cross-Flowing Channels	30:51	Cross-Flowing Channels of Biomass Sawdust	30:70	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	5	5	theme	β-cyclodextrin-polymer-functionalized	688:724	arg1	β-CD/WS					740:746	β-CD/WS	740:746	β-CD/WS	740:746	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	5	5	theme	β-cyclodextrin-polymer-functionalized	688:724	arg1	sawdust					731:737	β-cyclodextrin-polymer-functionalized wood sawdust	688:737	β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence	688:915	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	7	6	theme	β-CD/WS	1132:1138	arg1	device					1147:1152	the β-CD/WS filter device	1128:1152	the β-CD/WS filter device	1128:1152	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	0	7	theme	Pharmaceutical	103:116	arg1	Removal					129:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal	76:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water	76:146	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	5	8	from	micropores	846:855	arg1	surface					864:870	the surface	860:870	the surface of the cell walls	860:888	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	5	9	theme	wood	726:729	arg1	β-CD/WS					740:746	β-CD/WS	740:746	β-CD/WS	740:746	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	5	9	theme	wood	726:729	arg1	sawdust					731:737	β-cyclodextrin-polymer-functionalized wood sawdust	688:737	β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence	688:915	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	2	10	from	water	351:355	arg1	removal					307:313	rapid and efficient removal	287:313	rapid and efficient removal of pharmaceutical contaminants from water	287:355	However, rapid and efficient removal of pharmaceutical contaminants from water remains challenging.					
32603085	8	11	theme	global	1526:1531	arg1	issues					1549:1554	the global water pollution issues	1522:1554	the global water pollution issues	1522:1554	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	8	11	theme	global	1526:1531	arg1	those					1568:1572	those	1568:1572	those	1568:1572	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	6	12	theme	sawdust	1055:1061	arg1	backbone					1038:1045	the cellulose backbone	1024:1045	the cellulose backbone of wood sawdust	1024:1061	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	0	13	theme	Efficient	93:101	arg1	Removal					129:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal	76:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water	76:146	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	6	14	theme	full	957:960	arg1	contact					962:968	full contact	957:968	full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust	957:1061	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	2	15	theme	efficient	297:305	arg1	removal					307:313	rapid and efficient removal	287:313	rapid and efficient removal of pharmaceutical contaminants from water	287:355	However, rapid and efficient removal of pharmaceutical contaminants from water remains challenging.					
32603085	3	16	theme	unique	501:506	arg1	microstructure					530:543	a unique hierarchically porous microstructure	499:543	a unique hierarchically porous microstructure	499:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	17	theme	Wood	378:381	arg1	sawdust					383:389	Wood sawdust	378:389	Wood sawdust	378:389	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	17	theme	Wood	378:381	arg1	byproduct					394:402	a byproduct	392:402	a byproduct of the wood-processing industry	392:434	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	17	theme	Wood	378:381	arg1	material					485:492	an abundant, cost-effective, and sustainable material	440:492	an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure	440:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	7	18	theme	high	1345:1348	arg1	capacity					1368:1375	a high saturation uptake capacity	1343:1375	a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1	1343:1413	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	5	19	theme	cross-flow	647:656	arg1	composite					669:677	a novel cross-flow filtration composite	639:677	a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence	639:915	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	5	20	theme	novel	641:645	arg1	composite					669:677	a novel cross-flow filtration composite	639:677	a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence	639:915	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	7	21	dep	g-1	1411:1413	arg1	mg					1408:1409	mg	1408:1409	mg	1408:1409	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	3	22	theme	cost-effective	453:466	arg1	sawdust					383:389	Wood sawdust	378:389	Wood sawdust	378:389	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	22	theme	cost-effective	453:466	arg1	material					485:492	an abundant, cost-effective, and sustainable material	440:492	an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure	440:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	0	23	theme	Pollutants	118:127	arg1	Removal					129:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal	76:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water	76:146	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	5	24	theme	filtration	658:667	arg1	composite					669:677	a novel cross-flow filtration composite	639:677	a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence	639:915	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	2	25	theme	rapid	287:291	arg1	removal					307:313	rapid and efficient removal	287:313	rapid and efficient removal of pharmaceutical contaminants from water	287:355	However, rapid and efficient removal of pharmaceutical contaminants from water remains challenging.					
32603085	7	26	dep	mg	1408:1409	arg1	159					1395:1397	159	1395:1397	159	1395:1397	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	7	26	dep	mg	1408:1409	arg1	185					1404:1406	185	1404:1406	185	1404:1406	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	7	27	theme	pharmaceutical	1224:1237	arg1	contaminants					1239:1250	various pharmaceutical contaminants	1216:1250	various pharmaceutical contaminants	1216:1250	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	0	28	from	Channels	44:51	arg1	Polymerized					15:25	Polymerized	15:25	Polymerized	15:25	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	7	29	theme	uptake	1361:1366	arg1	capacity					1368:1375	a high saturation uptake capacity	1343:1375	a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1	1343:1413	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	5	30	theme	sawn-off	813:820	arg1	channels					829:836	the sawn-off vessel channels	809:836	the sawn-off vessel channels	809:836	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	3	31	theme	abundant	443:450	arg1	sawdust					383:389	Wood sawdust	378:389	Wood sawdust	378:389	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	31	theme	abundant	443:450	arg1	material					485:492	an abundant, cost-effective, and sustainable material	440:492	an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure	440:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	32	theme	wood-processing	411:425	arg1	industry					427:434	the wood-processing industry	407:434	the wood-processing industry	407:434	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	5	33	theme	pharmaceutical	762:775	arg1	water					789:793	the pharmaceutical contaminant water	758:793	the pharmaceutical contaminant water	758:793	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	2	34	theme	contaminants	333:344	arg1	removal					307:313	rapid and efficient removal	287:313	rapid and efficient removal of pharmaceutical contaminants from water	287:355	However, rapid and efficient removal of pharmaceutical contaminants from water remains challenging.					
32603085	8	35	theme	new	1498:1500	arg1	avenues					1502:1508	new avenues	1498:1508	new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants	1498:1610	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	0	36	theme	Cross-Flowing	30:42	arg1	Channels					44:51	Cross-Flowing Channels	30:51	Cross-Flowing Channels of Biomass Sawdust	30:70	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	7	37	theme	filter	1140:1145	arg1	device					1147:1152	the β-CD/WS filter device	1128:1152	the β-CD/WS filter device	1128:1152	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	2	38	theme	pharmaceutical	318:331	arg1	contaminants					333:344	pharmaceutical contaminants	318:344	pharmaceutical contaminants	318:344	However, rapid and efficient removal of pharmaceutical contaminants from water remains challenging.					
32603085	5	39	theme	walls	884:888	arg1	surface					864:870	the surface	860:870	the surface of the cell walls	860:888	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	5	40	theme	cell	879:882	arg1	walls					884:888	the cell walls	875:888	the cell walls	875:888	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	4	41	theme	filtration	602:611	arg1	material					613:620	a filtration material	600:620	a filtration material	600:620	These features make wood sawdust quite interesting as a filtration material.					
32603085	6	42	theme	water	923:927	arg1	characteristics					934:948	Such water flow characteristics	918:948	Such water flow characteristics	918:948	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	7	43	theme	saturation	1350:1359	arg1	capacity					1368:1375	a high saturation uptake capacity	1343:1375	a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1	1343:1413	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	3	44	with	material	485:492	arg1	microstructure					530:543	a unique hierarchically porous microstructure	499:543	a unique hierarchically porous microstructure	499:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	6	45	theme	Such	918:921	arg1	characteristics					934:948	Such water flow characteristics	918:948	Such water flow characteristics	918:948	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	7	46	theme	g-1	1411:1413	arg1	capacity					1368:1375	a high saturation uptake capacity	1343:1375	a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1	1343:1413	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	7	46	theme	g-1	1411:1413	arg1	efficiency					1175:1184	a high removal efficiency	1160:1184	a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin	1160:1336	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	1	47	theme	potential	230:238	arg1	risks					240:244	the potential risks	226:244	the potential risks for biosphere and human health	226:275	Water pollution arising from pharmaceuticals has raised great concerns about the potential risks for biosphere and human health.					
32603085	4	48	theme	wood	566:569	arg1	sawdust					571:577	wood sawdust	566:577	wood sawdust	566:577	These features make wood sawdust quite interesting as a filtration material.					
32603085	5	49	theme	contaminant	777:787	arg1	water					789:793	the pharmaceutical contaminant water	758:793	the pharmaceutical contaminant water	758:793	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	3	50	theme	sustainable	473:483	arg1	sawdust					383:389	Wood sawdust	378:389	Wood sawdust	378:389	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	50	theme	sustainable	473:483	arg1	material					485:492	an abundant, cost-effective, and sustainable material	440:492	an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure	440:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	51	theme	industry	427:434	arg1	sawdust					383:389	Wood sawdust	378:389	Wood sawdust	378:389	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	3	51	theme	industry	427:434	arg1	byproduct					394:402	a byproduct	392:402	a byproduct of the wood-processing industry	392:434	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	5	52	theme	vessel	822:827	arg1	channels					829:836	the sawn-off vessel channels	809:836	the sawn-off vessel channels	809:836	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	8	53	theme	high-performance	1434:1449	arg1	filtration					1481:1490	The high-performance wood-sawdust-based cross-flow filtration	1430:1490	The high-performance wood-sawdust-based cross-flow filtration	1430:1490	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	3	54	theme	hierarchically	508:521	arg1	microstructure					530:543	a unique hierarchically porous microstructure	499:543	a unique hierarchically porous microstructure	499:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	0	55	theme	Biomass	56:62	arg1	Sawdust					64:70	Biomass Sawdust	56:70	Biomass Sawdust	56:70	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	5	56	from	channels	829:836	arg1	surface					864:870	the surface	860:870	the surface of the cell walls	860:888	Here, we report a novel cross-flow filtration composite based on β-cyclodextrin-polymer-functionalized wood sawdust (β-CD/WS) in which the pharmaceutical contaminant water flows through the sawn-off vessel channels and the micropores on the surface of the cell walls, generating the turbulence.					
32603085	7	57	theme	removal	1167:1173	arg1	efficiency					1175:1184	a high removal efficiency	1160:1184	a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin	1160:1336	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	3	58	theme	porous	523:528	arg1	microstructure					530:543	a unique hierarchically porous microstructure	499:543	a unique hierarchically porous microstructure	499:543	Wood sawdust, a byproduct of the wood-processing industry, is an abundant, cost-effective, and sustainable material with a unique hierarchically porous microstructure.					
32603085	8	59	theme	cross-flow	1470:1479	arg1	filtration					1481:1490	The high-performance wood-sawdust-based cross-flow filtration	1430:1490	The high-performance wood-sawdust-based cross-flow filtration	1430:1490	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	7	60	theme	high	1162:1165	arg1	efficiency					1175:1184	a high removal efficiency	1160:1184	a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin	1160:1336	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	6	61	theme	treatment	1092:1100	arg1	efficiency					1102:1111	the water treatment efficiency	1082:1111	the water treatment efficiency	1082:1111	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	8	62	theme	pollution	1539:1547	arg1	issues					1549:1554	the global water pollution issues	1522:1554	the global water pollution issues	1522:1554	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	8	62	theme	pollution	1539:1547	arg1	those					1568:1572	those	1568:1572	those	1568:1572	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	8	63	theme	wood-sawdust-based	1451:1468	arg1	filtration					1481:1490	The high-performance wood-sawdust-based cross-flow filtration	1430:1490	The high-performance wood-sawdust-based cross-flow filtration	1430:1490	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	1	64	theme	Water	149:153	arg1	pollution					155:163	Water pollution	149:163	Water pollution arising from pharmaceuticals	149:192	Water pollution arising from pharmaceuticals has raised great concerns about the potential risks for biosphere and human health.					
32603085	6	65	theme	water	1086:1090	arg1	efficiency					1102:1111	the water treatment efficiency	1082:1111	the water treatment efficiency	1082:1111	Such water flow characteristics ensure full contact between pharmaceutical pollutants and β-CD grafted on the cellulose backbone of wood sawdust, thereby enhancing the water treatment efficiency.					
32603085	0	66	theme	Rapid	76:80	arg1	Removal					129:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal	76:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water	76:146	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	8	67	theme	water	1533:1537	arg1	issues					1549:1554	the global water pollution issues	1522:1554	the global water pollution issues	1522:1554	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	8	67	theme	water	1533:1537	arg1	those					1568:1572	those	1568:1572	those	1568:1572	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	8	68	theme	pharmaceutical	1584:1597	arg1	contaminants					1599:1610	pharmaceutical contaminants	1584:1610	pharmaceutical contaminants	1584:1610	The high-performance wood-sawdust-based cross-flow filtration opens new avenues for solving the global water pollution issues, especially those caused by pharmaceutical contaminants.					
32603085	0	69	from	Water	142:146	arg1	Removal					129:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal	76:135	Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water	76:146	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	0	70	theme	Sawdust	64:70	arg1	Channels					44:51	Cross-Flowing Channels	30:51	Cross-Flowing Channels of Biomass Sawdust	30:70	β-Cyclodextrin Polymerized in Cross-Flowing Channels of Biomass Sawdust for Rapid and Highly Efficient Pharmaceutical Pollutants Removal from Water.					
32603085	7	71	theme	various	1216:1222	arg1	contaminants					1239:1250	various pharmaceutical contaminants	1216:1250	various pharmaceutical contaminants	1216:1250	Consequently, the β-CD/WS filter device shows a high removal efficiency of over 97.5% within 90 s for various pharmaceutical contaminants including propranolol, amitriptyline, chlortetracycline, diclofenac, and levofloxacin, and a high saturation uptake capacity of 170, 156, 257, 159, and 185 mg g-1, respectively.					
32603085	1	72	theme	human	264:268	arg1	health					270:275	human health	264:275	human health	264:275	Water pollution arising from pharmaceuticals has raised great concerns about the potential risks for biosphere and human health.					
32512266	5	0	theme	anti-neuroinflammatory	799:820	arg1	activity					822:829	more potential anti-neuroinflammatory activity	784:829	more potential anti-neuroinflammatory activity	784:829	All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate.					
32512266	4	1	from	effect	678:683	arg1	accumulation					695:706	the NO accumulation	688:706	the NO accumulation induced by LPS in BV-2 cells	688:735	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	0	2	theme	microglia	77:85	arg1	cells					87:91	BV2 microglia cells	73:91	BV2 microglia cells	73:91	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	2	3	theme	biotransformation	339:355	arg1	metabolites					357:367	nine new biotransformation metabolites	330:367	nine new biotransformation metabolites (2-10)	330:374	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	2	3	theme	biotransformation	339:355	arg1	result					322:327	a result	320:327	a result	320:327	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	2	3	theme	biotransformation	339:355	arg1	2-10					370:373	2-10	370:373	2-10	370:373	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	0	4	theme	microbial	96:104	arg1	transformation					106:119	microbial transformation	96:119	microbial transformation of isorhodeasapogenin	96:141	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	4	5	dep	metabolites	608:618	arg1	2-6					620:622	2-6	620:622	2-6	620:622	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	3	6	theme	D	534:534	arg1	rings					542:546	the B, C, D and F rings	524:546	the B, C, D and F rings of steroidal skeleton	524:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	1	7	theme	fungus	272:277	arg1	racemosum					295:303	the fungus Syncephalastrum racemosum	268:303	the fungus Syncephalastrum racemosum (AS 3.264)	268:314	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	0	8	from	production	59:68	arg1	cells					87:91	BV2 microglia cells	73:91	BV2 microglia cells	73:91	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	4	9	theme	NO	692:693	arg1	accumulation					695:706	the NO accumulation	688:706	the NO accumulation induced by LPS in BV-2 cells	688:735	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	6	10	from	C-15	967:970	arg1	hydroxylation					942:954	hydroxylation	942:954	hydroxylation at C-12 or C-15	942:970	Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15.					
32512266	4	11	theme	biotransformed	593:606	arg1	metabolites					608:618	its biotransformed metabolites	589:618	its biotransformed metabolites 2-6, 8-10	589:628	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	3	12	theme	C	531:531	arg1	rings					542:546	the B, C, D and F rings	524:546	the B, C, D and F rings of steroidal skeleton	524:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	0	13	theme	anti-neuroinflammatory	4:25	arg1	steroids					27:34	New anti-neuroinflammatory steroids	0:34	New anti-neuroinflammatory steroids against LPS	0:46	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	7	14	theme	COX-2	1068:1072	arg1	expression					1045:1054	the LPS-induced protein expression	1021:1054	the LPS-induced protein expression of iNOS and COX-2	1021:1072	Moreover, metabolite 2 dose-dependently reduced the LPS-induced protein expression of iNOS and COX-2.					
32512266	7	15	theme	protein	1037:1043	arg1	expression					1045:1054	the LPS-induced protein expression	1021:1054	the LPS-induced protein expression of iNOS and COX-2	1021:1072	Moreover, metabolite 2 dose-dependently reduced the LPS-induced protein expression of iNOS and COX-2.					
32512266	0	16	theme	New	0:2	arg1	steroids					27:34	New anti-neuroinflammatory steroids	0:34	New anti-neuroinflammatory steroids against LPS	0:46	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	4	17	from	LPS	719:721	arg1	cells					731:735	BV-2 cells	726:735	BV-2 cells	726:735	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	3	18	theme	steroidal	551:559	arg1	skeleton					561:568	steroidal skeleton	551:568	steroidal skeleton	551:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	1	19	theme	major	200:204	arg1	sapogenin					216:224	the major steroidal sapogenin	196:224	the major steroidal sapogenin of Tupistra chinensis	196:246	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	1	19	theme	major	200:204	arg1	isorhodeasapogenin					172:189	isorhodeasapogenin (1)	172:193	isorhodeasapogenin (1)	172:193	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	2	20	theme	spectroscopic	430:442	arg1	analysis					444:451	spectroscopic analysis	430:451	spectroscopic analysis	430:451	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	7	21	theme	iNOS	1059:1062	arg1	expression					1045:1054	the LPS-induced protein expression	1021:1054	the LPS-induced protein expression of iNOS and COX-2	1021:1072	Moreover, metabolite 2 dose-dependently reduced the LPS-induced protein expression of iNOS and COX-2.					
32512266	3	22	theme	F	540:540	arg1	rings					542:546	the B, C, D and F rings	524:546	the B, C, D and F rings of steroidal skeleton	524:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	1	23	theme	steroidal	206:214	arg1	sapogenin					216:224	the major steroidal sapogenin	196:224	the major steroidal sapogenin of Tupistra chinensis	196:246	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	1	23	theme	steroidal	206:214	arg1	isorhodeasapogenin					172:189	isorhodeasapogenin (1)	172:193	isorhodeasapogenin (1)	172:193	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	5	24	theme	potential	789:797	arg1	activity					822:829	more potential anti-neuroinflammatory activity	784:829	more potential anti-neuroinflammatory activity	784:829	All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate.					
32512266	0	25	theme	isorhodeasapogenin	124:141	arg1	transformation					106:119	microbial transformation	96:119	microbial transformation of isorhodeasapogenin	96:141	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	4	26	theme	anti-neuroinflammatory	655:676	arg1	effect					678:683	their anti-neuroinflammatory effect	649:683	their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells	649:735	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	5	27	theme	tested	746:751	arg1	metabolites					753:763	All the tested metabolites	738:763	All the tested metabolites	738:763	All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate.					
32512266	6	28	from	C-12	959:962	arg1	hydroxylation					942:954	hydroxylation	942:954	hydroxylation at C-12 or C-15	942:970	Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15.					
32512266	3	29	theme	Hydroxylation	454:466	arg1	reactions					497:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	5	30	contain	have	779:782	arg1	metabolites					753:763	All the tested metabolites	738:763	All the tested metabolites	738:763	All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate.					
32512266	5	30	contain	have	779:782	arg2	activity					822:829	more potential anti-neuroinflammatory activity	784:829	more potential anti-neuroinflammatory activity	784:829	All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate.					
32512266	6	31	theme	NO	922:923	arg1	production					925:934	NO production	922:934	NO production	922:934	Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15.					
32512266	7	32	theme	LPS-induced	1025:1035	arg1	expression					1045:1054	the LPS-induced protein expression	1021:1054	the LPS-induced protein expression of iNOS and COX-2	1021:1072	Moreover, metabolite 2 dose-dependently reduced the LPS-induced protein expression of iNOS and COX-2.					
32512266	3	33	theme	skeleton	561:568	arg1	rings					542:546	the B, C, D and F rings	524:546	the B, C, D and F rings of steroidal skeleton	524:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	2	34	theme	new	335:337	arg1	metabolites					357:367	nine new biotransformation metabolites	330:367	nine new biotransformation metabolites (2-10)	330:374	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	2	34	theme	new	335:337	arg1	result					322:327	a result	320:327	a result	320:327	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	2	34	theme	new	335:337	arg1	2-10					370:373	2-10	370:373	2-10	370:373	As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis.					
32512266	6	35	from	inhibition	908:917	arg1	production					925:934	NO production	922:934	NO production	922:934	Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15.					
32512266	1	36	dep	fungus	272:277	arg1	Syncephalastrum					279:293	Syncephalastrum	279:293	Syncephalastrum	279:293	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	1	37	theme	Microbial	144:152	arg1	transformation					154:167	Microbial transformation	144:167	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis,	144:247	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	4	38	theme	BV-2	726:729	arg1	cells					731:735	BV-2 cells	726:735	BV-2 cells	726:735	Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells.					
32512266	1	39	theme	chinensis	238:246	arg1	sapogenin					216:224	the major steroidal sapogenin	196:224	the major steroidal sapogenin of Tupistra chinensis	196:246	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	1	39	theme	chinensis	238:246	arg1	isorhodeasapogenin					172:189	isorhodeasapogenin (1)	172:193	isorhodeasapogenin (1)	172:193	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32512266	3	40	located	observed	512:519	arg1	rings					542:546	the B, C, D and F rings	524:546	the B, C, D and F rings of steroidal skeleton	524:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	3	40	located	observed	512:519	arg2	reactions					497:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	3	41	theme	glycosylation	483:495	arg1	reactions					497:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	0	42	theme	BV2	73:75	arg1	cells					87:91	BV2 microglia cells	73:91	BV2 microglia cells	73:91	New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.					
32512266	3	43	theme	oxidation	469:477	arg1	reactions					497:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions	454:505	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	3	44	theme	B	528:528	arg1	rings					542:546	the B, C, D and F rings	524:546	the B, C, D and F rings of steroidal skeleton	524:568	Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton.					
32512266	6	45	theme	significant	896:906	arg1	inhibition					908:917	significant inhibition	896:917	significant inhibition on NO production	896:934	Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15.					
32512266	1	46	theme	isorhodeasapogenin	172:189	arg1	transformation					154:167	Microbial transformation	144:167	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis,	144:247	Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264).					
32679636	14	0	theme	considerable	2158:2169	arg1	reusability					2171:2181	considerable reusability	2158:2181	considerable reusability	2158:2181	The developed nanocomposite showed considerable reusability up to 3 cylces of the batch adsorption process.					
32679636	3	1	theme	genetic	515:521	arg1	programming					523:533	the genetic programming	511:533	the genetic programming	511:533	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	2	theme	adsorbent	720:728	arg1	dose					730:733	adsorbent dose	720:733	adsorbent dose	720:733	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	13	3	theme	dyes	2101:2104	arg1	removal					2085:2091	higher removal	2078:2091	higher removal of both dyes	2078:2104	Furthermore, the interaction energies calculated by DFT confirmed the experimental adsorption data, where PPy/CMC resulted in higher removal of both dyes compared to PPy.					
32679636	6	4	theme	experimental	1106:1117	arg1	data					1130:1133	the experimental adsorption data	1102:1133	the experimental adsorption data	1102:1133	Also, the results indicated that ANN method could predict the experimental adsorption data with higher accuracy than other methods.					
32679636	3	5	theme	surface	545:551	arg1	methodology					553:563	response surface methodology	536:563	response surface methodology	536:563	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	1	6	theme	polypyrrole/carboxymethyl	188:212	arg1	dyes					374:377	highly toxic dyes	361:377	highly toxic dyes	361:377	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	6	theme	polypyrrole/carboxymethyl	188:212	arg1	particles					238:246	polypyrrole/carboxymethyl cellulose nanocomposite particles	188:246	polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs)	188:260	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	6	theme	polypyrrole/carboxymethyl	188:212	arg1	NPs					257:259	PPy/CMC NPs	249:259	PPy/CMC NPs	249:259	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	11	7	theme	more	1736:1739	arg1	insight					1741:1747	more insight	1736:1747	more insight into the interactions between the dyes and adsorbent	1736:1800	Moreover, DFT calculations were carried out to obtain more insight into the interactions between the dyes and adsorbent.					
32679636	1	8	theme	nanocomposite	224:236	arg1	dyes					374:377	highly toxic dyes	361:377	highly toxic dyes	361:377	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	8	theme	nanocomposite	224:236	arg1	particles					238:246	polypyrrole/carboxymethyl cellulose nanocomposite particles	188:246	polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs)	188:260	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	8	theme	nanocomposite	224:236	arg1	NPs					257:259	PPy/CMC NPs	249:259	PPy/CMC NPs	249:259	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	12	9	theme	studied	1916:1922	arg1	dyes					1924:1927	the two studied dyes	1908:1927	the two studied dyes	1908:1927	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	3	10	theme	artificial	570:579	arg1	ANN					597:599	ANN	597:599	ANN	597:599	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	10	theme	artificial	570:579	arg1	network					588:594	artificial neural network	570:594	artificial neural network (ANN)	570:600	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	0	11	theme	DFT	156:158	arg1	dyes					120:123	reactive dyes	111:123	reactive dyes: Experimental optimization and DFT calculation	111:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	11	theme	DFT	156:158	arg1	calculation					160:170	DFT calculation	156:170	DFT calculation	156:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	4	12	theme	55 min	862:867	arg1	dose					966:969	adsorbent dose	956:969	adsorbent dose of 0.09 g for both dyes	956:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	12	theme	55 min	862:867	arg1	concentration					926:938	initial dye concentration	914:938	initial dye concentration of 100 mg/L	914:950	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	12	theme	55 min	862:867	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	12	theme	55 min	862:867	arg1	time					854:857	adsorption time	843:857	adsorption time of 55 min and 52 min for RR56 and RB160, respectively	843:911	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	13	13	theme	adsorption	2035:2044	arg1	data					2046:2049	the experimental adsorption data	2018:2049	the experimental adsorption data	2018:2049	Furthermore, the interaction energies calculated by DFT confirmed the experimental adsorption data, where PPy/CMC resulted in higher removal of both dyes compared to PPy.					
32679636	4	14	theme	52 min	873:878	arg1	dose					966:969	adsorbent dose	956:969	adsorbent dose of 0.09 g for both dyes	956:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	14	theme	52 min	873:878	arg1	concentration					926:938	initial dye concentration	914:938	initial dye concentration of 100 mg/L	914:950	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	14	theme	52 min	873:878	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	14	theme	52 min	873:878	arg1	time					854:857	adsorption time	843:857	adsorption time of 55 min and 52 min for RR56 and RB160, respectively	843:911	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	8	15	theme	removal	1395:1401	arg1	process					1403:1409	the RB160 removal process	1385:1409	the RB160 removal process	1385:1409	However, initial concentration mostly determines the RB160 removal process.					
32679636	6	16	theme	higher	1140:1145	arg1	accuracy					1147:1154	higher accuracy	1140:1154	higher accuracy than other methods	1140:1173	Also, the results indicated that ANN method could predict the experimental adsorption data with higher accuracy than other methods.					
32679636	0	17	theme	nanoparticles	82:94	arg1	capacity					44:51	adsorption capacity	33:51	adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation	33:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	4	18	theme	100 mg/L	943:950	arg1	dose					966:969	adsorbent dose	956:969	adsorbent dose of 0.09 g for both dyes	956:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	18	theme	100 mg/L	943:950	arg1	concentration					926:938	initial dye concentration	914:938	initial dye concentration of 100 mg/L	914:950	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	18	theme	100 mg/L	943:950	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	18	theme	100 mg/L	943:950	arg1	time					854:857	adsorption time	843:857	adsorption time of 55 min and 52 min for RR56 and RB160, respectively	843:911	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	13	19	theme	experimental	2022:2033	arg1	data					2046:2049	the experimental adsorption data	2018:2049	the experimental adsorption data	2018:2049	Furthermore, the interaction energies calculated by DFT confirmed the experimental adsorption data, where PPy/CMC resulted in higher removal of both dyes compared to PPy.					
32679636	9	20	theme	Langmuir	1457:1464	arg1	model					1475:1479	the Langmuir isotherm model	1453:1479	the Langmuir isotherm model	1453:1479	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	4	21	theme	RR56	762:765	arg1	adsorption					748:757	The maximum adsorption	736:757	The maximum adsorption of RR56 and RB160	736:775	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	0	22	theme	reactive	111:118	arg1	dyes					120:123	reactive dyes	111:123	reactive dyes: Experimental optimization and DFT calculation	111:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	22	theme	reactive	111:118	arg1	optimization					139:150	Experimental optimization	126:150	Experimental optimization	126:150	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	22	theme	reactive	111:118	arg1	calculation					160:170	DFT calculation	156:170	DFT calculation	156:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	13	23	theme	interaction	1969:1979	arg1	energies					1981:1988	the interaction energies	1965:1988	the interaction energies calculated by DFT	1965:2006	Furthermore, the interaction energies calculated by DFT confirmed the experimental adsorption data, where PPy/CMC resulted in higher removal of both dyes compared to PPy.					
32679636	12	24	theme	der	1857:1859	arg1	interactions					1867:1878	Van der Waals interactions	1853:1878	Van der Waals interactions	1853:1878	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	24	theme	der	1857:1859	arg1	forces					1893:1898	dominant forces	1884:1898	dominant forces between the two studied dyes and PPy/CMC composite	1884:1949	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	24	theme	der	1857:1859	arg1	bondings					1840:1847	the hydrogen bondings	1827:1847	the hydrogen bondings	1827:1847	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	4	25	theme	initial	914:920	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	25	theme	initial	914:920	arg1	concentration					926:938	initial dye concentration	914:938	initial dye concentration of 100 mg/L	914:950	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	26	theme	RB160	771:775	arg1	adsorption					748:757	The maximum adsorption	736:757	The maximum adsorption of RR56 and RB160	736:775	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	14	27	theme	process	2222:2228	arg1	cylces					2191:2196	3 cylces	2189:2196	3 cylces of the batch adsorption process	2189:2228	The developed nanocomposite showed considerable reusability up to 3 cylces of the batch adsorption process.					
32679636	14	28	theme	batch	2205:2209	arg1	process					2222:2228	the batch adsorption process	2201:2228	the batch adsorption process	2201:2228	The developed nanocomposite showed considerable reusability up to 3 cylces of the batch adsorption process.					
32679636	0	29	theme	Carboxymethyl	0:12	arg1	cellulose					14:22	Carboxymethyl cellulose	0:22	Carboxymethyl cellulose	0:22	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	12	30	theme	hydrogen	1831:1838	arg1	interactions					1867:1878	Van der Waals interactions	1853:1878	Van der Waals interactions	1853:1878	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	30	theme	hydrogen	1831:1838	arg1	bondings					1840:1847	the hydrogen bondings	1827:1847	the hydrogen bondings	1827:1847	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	30	theme	hydrogen	1831:1838	arg1	forces					1893:1898	dominant forces	1884:1898	dominant forces between the two studied dyes and PPy/CMC composite	1884:1949	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	9	31	theme	adsorption	1496:1505	arg1	capacity					1507:1514	a maximum adsorption capacity	1486:1514	a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively	1486:1576	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	7	32	theme	main	1245:1248	arg1	dose					1233:1236	the adsorbent dose	1219:1236	the adsorbent dose	1219:1236	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	7	32	theme	main	1245:1248	arg1	factor					1250:1255	the main factor	1241:1255	the main factor in RR56 removal	1241:1271	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	4	33	theme	adsorbent	956:964	arg1	dose					966:969	adsorbent dose	956:969	adsorbent dose of 0.09 g for both dyes	956:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	33	theme	adsorbent	956:964	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	3	34	theme	parameters	648:657	arg1	effect					628:633	the effect	624:633	the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose	624:733	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	4	35	theme	optimum	808:814	arg1	conditions					816:825	the following optimum conditions	794:825	the following optimum conditions	794:825	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	10	36	theme	thermodynamic	1592:1604	arg1	studies					1606:1612	thermodynamic studies	1592:1612	thermodynamic studies	1592:1612	In addition, thermodynamic studies indicated the endothermic adsorption process for both studied dyes.					
32679636	1	37	theme	RR56	323:326	arg1	removal					295:301	removal	295:301	removal of reactive red 56 (RR56)and reactive blue 160 (RB160)	295:356	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	2	38	theme	adsorption	447:456	arg1	efficiency					458:467	the surface adsorption efficiency	435:467	the surface adsorption efficiency	435:467	The amount of CMC was found significantly effective on the surface adsorption efficiency.					
32679636	0	39	theme	polypyrrole/CMC	56:70	arg1	nanoparticles					82:94	polypyrrole/CMC composite nanoparticles	56:94	polypyrrole/CMC composite nanoparticles	56:94	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	7	40	theme	adsorbent	1223:1231	arg1	dose					1233:1236	the adsorbent dose	1219:1236	the adsorbent dose	1219:1236	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	7	40	theme	adsorbent	1223:1231	arg1	factor					1250:1255	the main factor	1241:1255	the main factor in RR56 removal	1241:1271	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	3	41	theme	adsorption	673:682	arg1	time					684:687	adsorption time	673:687	adsorption time	673:687	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	42	theme	Different	470:478	arg1	methods					493:499	Different optimization methods	470:499	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN)	470:600	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	7	43	from	factor	1250:1255	arg1	removal					1265:1271	RR56 removal	1260:1271	RR56 removal	1260:1271	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	7	44	theme	results	1196:1202	arg1	analysis					1180:1187	The analysis	1176:1187	The analysis of ANN results	1176:1202	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	13	45	dep	resulted	2066:2073	arg1	compared					2106:2113	compared	2106:2113	compared to PPy	2106:2120	Furthermore, the interaction energies calculated by DFT confirmed the experimental adsorption data, where PPy/CMC resulted in higher removal of both dyes compared to PPy.					
32679636	3	46	theme	dye	698:700	arg1	concentration					702:714	initial dye concentration	690:714	initial dye concentration	690:714	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	4	47	theme	5	840:840	arg1	pH					828:829	pH	828:829	pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes	828:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	48	theme	adsorption	843:852	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	48	theme	adsorption	843:852	arg1	time					854:857	adsorption time	843:857	adsorption time of 55 min and 52 min for RR56 and RB160, respectively	843:911	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	6	49	theme	adsorption	1119:1128	arg1	data					1130:1133	the experimental adsorption data	1102:1133	the experimental adsorption data	1102:1133	Also, the results indicated that ANN method could predict the experimental adsorption data with higher accuracy than other methods.					
32679636	14	50	theme	developed	2127:2135	arg1	nanocomposite					2137:2149	The developed nanocomposite	2123:2149	The developed nanocomposite	2123:2149	The developed nanocomposite showed considerable reusability up to 3 cylces of the batch adsorption process.					
32679636	3	51	theme	response	536:543	arg1	methodology					553:563	response surface methodology	536:563	response surface methodology	536:563	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	1	52	theme	cellulose	214:222	arg1	dyes					374:377	highly toxic dyes	361:377	highly toxic dyes	361:377	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	52	theme	cellulose	214:222	arg1	particles					238:246	polypyrrole/carboxymethyl cellulose nanocomposite particles	188:246	polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs)	188:260	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	52	theme	cellulose	214:222	arg1	NPs					257:259	PPy/CMC NPs	249:259	PPy/CMC NPs	249:259	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	12	53	theme	PPy/CMC	1933:1939	arg1	composite					1941:1949	PPy/CMC composite	1933:1949	PPy/CMC composite	1933:1949	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	3	54	theme	neural	581:586	arg1	ANN					597:599	ANN	597:599	ANN	597:599	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	54	theme	neural	581:586	arg1	network					588:594	artificial neural network	570:594	artificial neural network (ANN)	570:600	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	4	55	dep	found	782:786	arg1	pH					828:829	pH	828:829	pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes	828:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	56	theme	4	834:834	arg1	pH					828:829	pH	828:829	pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes	828:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	6	57	theme	ANN	1077:1079	arg1	method					1081:1086	ANN method	1077:1086	ANN method	1077:1086	Also, the results indicated that ANN method could predict the experimental adsorption data with higher accuracy than other methods.					
32679636	1	58	theme	PPy/CMC	249:255	arg1	particles					238:246	polypyrrole/carboxymethyl cellulose nanocomposite particles	188:246	polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs)	188:260	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	58	theme	PPy/CMC	249:255	arg1	NPs					257:259	PPy/CMC NPs	249:259	PPy/CMC NPs	249:259	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	8	59	theme	RB160	1389:1393	arg1	process					1403:1409	the RB160 removal process	1385:1409	the RB160 removal process	1385:1409	However, initial concentration mostly determines the RB160 removal process.					
32679636	7	60	theme	initial	1299:1305	arg1	concentration					1307:1319	initial concentration	1299:1319	initial concentration	1299:1319	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	13	61	theme	higher	2078:2083	arg1	removal					2085:2091	higher removal	2078:2091	higher removal of both dyes	2078:2104	Furthermore, the interaction energies calculated by DFT confirmed the experimental adsorption data, where PPy/CMC resulted in higher removal of both dyes compared to PPy.					
32679636	9	62	theme	isotherm	1416:1423	arg1	data					1425:1428	The isotherm data	1412:1428	The isotherm data for both dyes	1412:1442	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	12	63	theme	dominant	1884:1891	arg1	bondings					1840:1847	the hydrogen bondings	1827:1847	the hydrogen bondings	1827:1847	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	63	theme	dominant	1884:1891	arg1	forces					1893:1898	dominant forces	1884:1898	dominant forces between the two studied dyes and PPy/CMC composite	1884:1949	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	63	theme	dominant	1884:1891	arg1	interactions					1867:1878	Van der Waals interactions	1853:1878	Van der Waals interactions	1853:1878	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	6	64	theme	other	1161:1165	arg1	methods					1167:1173	other methods	1161:1173	other methods	1161:1173	Also, the results indicated that ANN method could predict the experimental adsorption data with higher accuracy than other methods.					
32679636	11	65	theme	DFT	1692:1694	arg1	calculations					1696:1707	DFT calculations	1692:1707	DFT calculations	1692:1707	Moreover, DFT calculations were carried out to obtain more insight into the interactions between the dyes and adsorbent.					
32679636	4	66	theme	dye	922:924	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	66	theme	dye	922:924	arg1	concentration					926:938	initial dye concentration	914:938	initial dye concentration of 100 mg/L	914:950	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	3	67	used	used	607:610	arg2	methods					493:499	Different optimization methods	470:499	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN)	470:600	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	12	68	theme	Van	1853:1855	arg1	interactions					1867:1878	Van der Waals interactions	1853:1878	Van der Waals interactions	1853:1878	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	68	theme	Van	1853:1855	arg1	forces					1893:1898	dominant forces	1884:1898	dominant forces between the two studied dyes and PPy/CMC composite	1884:1949	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	68	theme	Van	1853:1855	arg1	bondings					1840:1847	the hydrogen bondings	1827:1847	the hydrogen bondings	1827:1847	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	10	69	theme	endothermic	1628:1638	arg1	process					1651:1657	the endothermic adsorption process	1624:1657	the endothermic adsorption process for both studied dyes	1624:1679	In addition, thermodynamic studies indicated the endothermic adsorption process for both studied dyes.					
32679636	0	70	theme	dyes	120:123	arg1	removal					100:106	removal	100:106	removal of reactive dyes: Experimental optimization and DFT calculation	100:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	1	71	theme	RB160	351:355	arg1	removal					295:301	removal	295:301	removal of reactive red 56 (RR56)and reactive blue 160 (RB160)	295:356	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	12	72	theme	Waals	1861:1865	arg1	interactions					1867:1878	Van der Waals interactions	1853:1878	Van der Waals interactions	1853:1878	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	72	theme	Waals	1861:1865	arg1	forces					1893:1898	dominant forces	1884:1898	dominant forces between the two studied dyes and PPy/CMC composite	1884:1949	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	12	72	theme	Waals	1861:1865	arg1	bondings					1840:1847	the hydrogen bondings	1827:1847	the hydrogen bondings	1827:1847	The results showed that the hydrogen bondings and Van der Waals interactions are dominant forces between the two studied dyes and PPy/CMC composite.					
32679636	9	73	theme	isotherm	1466:1473	arg1	model					1475:1479	the Langmuir isotherm model	1453:1479	the Langmuir isotherm model	1453:1479	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	0	74	theme	Experimental	126:137	arg1	dyes					120:123	reactive dyes	111:123	reactive dyes: Experimental optimization and DFT calculation	111:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	74	theme	Experimental	126:137	arg1	optimization					139:150	Experimental optimization	126:150	Experimental optimization	126:150	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	75	dep	dyes	120:123	arg1	dyes					120:123	reactive dyes	111:123	reactive dyes: Experimental optimization and DFT calculation	111:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	75	dep	dyes	120:123	arg1	optimization					139:150	Experimental optimization	126:150	Experimental optimization	126:150	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	0	75	dep	dyes	120:123	arg1	calculation					160:170	DFT calculation	156:170	DFT calculation	156:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	7	76	theme	RR56	1260:1263	arg1	removal					1265:1271	RR56 removal	1260:1271	RR56 removal	1260:1271	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	2	77	theme	CMC	394:396	arg1	CMC					394:396	CMC	394:396	CMC	394:396	The amount of CMC was found significantly effective on the surface adsorption efficiency.					
32679636	2	77	theme	CMC	394:396	arg1	amount					384:389	The amount	380:389	The amount of CMC	380:396	The amount of CMC was found significantly effective on the surface adsorption efficiency.					
32679636	14	78	theme	adsorption	2211:2220	arg1	process					2222:2228	the batch adsorption process	2201:2228	the batch adsorption process	2201:2228	The developed nanocomposite showed considerable reusability up to 3 cylces of the batch adsorption process.					
32679636	1	79	theme	toxic	368:372	arg1	dyes					374:377	highly toxic dyes	361:377	highly toxic dyes	361:377	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	1	79	theme	toxic	368:372	arg1	particles					238:246	polypyrrole/carboxymethyl cellulose nanocomposite particles	188:246	polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs)	188:260	In this study, polypyrrole/carboxymethyl cellulose nanocomposite particles (PPy/CMC NPs) were synthesized and applied for removal of reactive red 56 (RR56)and reactive blue 160 (RB160) as highly toxic dyes.					
32679636	9	80	theme	maximum	1488:1494	arg1	capacity					1507:1514	a maximum adsorption capacity	1486:1514	a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively	1486:1576	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	4	81	theme	0.09 g	974:979	arg1	dose					966:969	adsorbent dose	956:969	adsorbent dose of 0.09 g for both dyes	956:993	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	81	theme	0.09 g	974:979	arg1	concentration					926:938	initial dye concentration	914:938	initial dye concentration of 100 mg/L	914:950	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	81	theme	0.09 g	974:979	arg1	4					834:834	4	834:834	4	834:834	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	4	81	theme	0.09 g	974:979	arg1	time					854:857	adsorption time	843:857	adsorption time of 55 min and 52 min for RR56 and RB160, respectively	843:911	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	0	82	theme	adsorption	33:42	arg1	capacity					44:51	adsorption capacity	33:51	adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation	33:170	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	3	83	theme	different	638:646	arg1	pH					669:670	pH	669:670	pH	669:670	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	83	theme	different	638:646	arg1	time					684:687	adsorption time	673:687	adsorption time	673:687	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	83	theme	different	638:646	arg1	parameters					648:657	different parameters	638:657	different parameters including pH, adsorption time, initial dye concentration and adsorbent dose	638:733	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	83	theme	different	638:646	arg1	concentration					702:714	initial dye concentration	690:714	initial dye concentration	690:714	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	3	83	theme	different	638:646	arg1	dose					730:733	adsorbent dose	720:733	adsorbent dose	720:733	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	4	84	theme	maximum	740:746	arg1	adsorption					748:757	The maximum adsorption	736:757	The maximum adsorption of RR56 and RB160	736:775	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	9	85	theme	104.9 mg/g	1519:1528	arg1	capacity					1507:1514	a maximum adsorption capacity	1486:1514	a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively	1486:1576	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	4	86	theme	following	798:806	arg1	conditions					816:825	the following optimum conditions	794:825	the following optimum conditions	794:825	The maximum adsorption of RR56 and RB160 were found under the following optimum conditions: pH of 4 and 5, adsorption time of 55 min and 52 min for RR56 and RB160, respectively, initial dye concentration of 100 mg/L and adsorbent dose of 0.09 g for both dyes.					
32679636	0	87	theme	composite	72:80	arg1	nanoparticles					82:94	polypyrrole/CMC composite nanoparticles	56:94	polypyrrole/CMC composite nanoparticles	56:94	Carboxymethyl cellulose improved adsorption capacity of polypyrrole/CMC composite nanoparticles for removal of reactive dyes: Experimental optimization and DFT calculation.					
32679636	9	88	theme	120.7 mg/g	1534:1543	arg1	capacity					1507:1514	a maximum adsorption capacity	1486:1514	a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively	1486:1576	The isotherm data for both dyes followed the Langmuir isotherm model with a maximum adsorption capacity of 104.9 mg/g and 120.7 mg/g for RR56 and RB160, respectively.					
32679636	2	89	theme	surface	439:445	arg1	efficiency					458:467	the surface adsorption efficiency	435:467	the surface adsorption efficiency	435:467	The amount of CMC was found significantly effective on the surface adsorption efficiency.					
32679636	10	90	theme	adsorption	1640:1649	arg1	process					1651:1657	the endothermic adsorption process	1624:1657	the endothermic adsorption process for both studied dyes	1624:1679	In addition, thermodynamic studies indicated the endothermic adsorption process for both studied dyes.					
32679636	3	91	theme	optimization	480:491	arg1	methods					493:499	Different optimization methods	470:499	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN)	470:600	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	7	92	theme	ANN	1192:1194	arg1	results					1196:1202	ANN results	1192:1202	ANN results	1192:1202	The analysis of ANN results indicated that the adsorbent dose is the main factor in RR56 removal, followed by time, pH and initial concentration, respectively.					
32679636	3	93	theme	initial	690:696	arg1	concentration					702:714	initial dye concentration	690:714	initial dye concentration	690:714	Different optimization methods including the genetic programming, response surface methodology, and artificial neural network (ANN) were used to optimize the effect of different parameters including pH, adsorption time, initial dye concentration and adsorbent dose.					
32679636	8	94	theme	initial	1345:1351	arg1	concentration					1353:1365	initial concentration	1345:1365	initial concentration	1345:1365	However, initial concentration mostly determines the RB160 removal process.					
32679636	10	95	theme	studied	1668:1674	arg1	dyes					1676:1679	both studied dyes	1663:1679	both studied dyes	1663:1679	In addition, thermodynamic studies indicated the endothermic adsorption process for both studied dyes.					
32572043	2	0	theme	depth	390:394	arg1	Effects					370:376	Effects	370:376	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition	370:454	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	6	1	theme	R-phycoerythrin	989:1003	arg1	contents					1012:1019	R-phycoerythrin (R-PE) contents	989:1019	R-phycoerythrin (R-PE) contents	989:1019	R-phycoerythrin (R-PE) contents were significantly higher (p < 0.05) in the BB (0.86 ± 0.31 mg g-1) than those obtained in the BL (0.33 ± 0.12 mg g-1).					
32572043	5	2	theme	proteins	851:858	arg1	%					932:932	20.74 ± 7.22%	920:932	20.74 ± 7.22%	920:932	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	2	theme	proteins	851:858	arg1	%					950:950	22.02 ± 6.34%	938:950	22.02 ± 6.34% for the BL and the BB	938:972	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	2	theme	proteins	851:858	arg1	independent					874:884	independent	874:884	independent	874:884	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	2	theme	proteins	851:858	arg1	contents					860:867	The proteins contents	847:867	The proteins contents	847:867	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	3	theme	site	893:896	arg1	%					932:932	20.74 ± 7.22%	920:932	20.74 ± 7.22%	920:932	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	3	theme	site	893:896	arg1	%					950:950	22.02 ± 6.34%	938:950	22.02 ± 6.34% for the BL and the BB	938:972	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	3	theme	site	893:896	arg1	independent					874:884	independent	874:884	independent	874:884	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	3	theme	site	893:896	arg1	contents					860:867	The proteins contents	847:867	The proteins contents	847:867	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	3	4	theme	1.19 ± 0.34	536:546	arg1	%					547:547	%	547:547	%	547:547	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	4	5	dep	higher	608:613	arg1	%					661:661	23.31 ± 2.64%	649:661	23.31 ± 2.64%	649:661	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	5	dep	higher	608:613	arg1	%					679:679	19.19 ± 2.32%	667:679	19.19 ± 2.32%	667:679	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	5	dep	higher	608:613	arg1	p < 0.05					616:623	p < 0.05	616:623	p < 0.05	616:623	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	6	dep	contents	737:744	arg1	%					759:759	41.37 ± 3.68%	747:759	41.37 ± 3.68%	747:759	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	6	dep	contents	737:744	arg1	%					776:776	23.30 ± 5.40%	764:776	23.30 ± 5.40%	764:776	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	0	7	from	Effect	0:5	arg1	Growth					60:65	Gracilaria gracilis Growth	40:65	Gracilaria gracilis Growth	40:65	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	0	7	from	Effect	0:5	arg1	Composition					83:93	Biochemical Composition	71:93	Biochemical Composition	71:93	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	4	8	theme	higher	689:694	arg1	3,6-AG					729:734	3,6-AG	729:734	3,6-AG	729:734	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	8	theme	higher	689:694	arg1	3,6-anhydrogalactose					707:726	a higher (p < 0.05) 3,6-anhydrogalactose	687:726	a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%)	687:777	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	0	9	theme	Biochemical	71:81	arg1	Composition					83:93	Biochemical Composition	71:93	Biochemical Composition	71:93	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	2	10	from	Effects	370:376	arg1	DGR					423:425	DGR	423:425	DGR	423:425	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	2	10	from	Effects	370:376	arg1	rate					417:420	alga daily growth rate	399:420	alga daily growth rate (DGR)	399:426	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	2	10	from	Effects	370:376	arg1	composition					444:454	biochemical composition	432:454	biochemical composition	432:454	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	1	11	theme	lantern	356:362	arg1	nets					364:367	lantern nets	356:367	lantern nets	356:367	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	2	12	theme	growth	410:415	arg1	DGR					423:425	DGR	423:425	DGR	423:425	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	2	12	theme	growth	410:415	arg1	rate					417:420	alga daily growth rate	399:420	alga daily growth rate (DGR)	399:426	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	1	13	theme	Bizerte	302:308	arg1	BB					315:316	BB	315:316	BB	315:316	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	13	theme	Bizerte	302:308	arg1	Bay					310:312	Bizerte Bay	302:312	Bizerte Bay (BB)	302:317	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	2	14	theme	daily	404:408	arg1	DGR					423:425	DGR	423:425	DGR	423:425	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	2	14	theme	daily	404:408	arg1	rate					417:420	alga daily growth rate	399:420	alga daily growth rate (DGR)	399:426	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	2	15	theme	site	381:384	arg1	Effects					370:376	Effects	370:376	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition	370:454	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	1	16	from	Bay	310:312	arg1	growth					149:154	the growth	145:154	the growth	145:154	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	16	from	Bay	310:312	arg1	composition					172:182	biochemical composition	160:182	biochemical composition	160:182	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	8	17	used	used	1325:1328	arg2	gracilis					1285:1292	G. gracilis	1282:1292	G. gracilis cultured in Bizerte Bay	1282:1316	Our results suggest that G. gracilis cultured in Bizerte Bay can be used in a cascading biorefinery approach.					
32572043	0	18	theme	gracilis	51:58	arg1	Growth					60:65	Gracilaria gracilis Growth	40:65	Gracilaria gracilis Growth	40:65	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	1	19	theme	biochemical	160:170	arg1	composition					172:182	biochemical composition	160:182	biochemical composition	160:182	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	5	20	dep	site	893:896	arg1	the					889:891	the	889:891	the	889:891	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	4	21	theme	Agar	592:595	arg1	yield					597:601	Agar yield	592:601	Agar yield	592:601	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	2	22	theme	biochemical	432:442	arg1	composition					444:454	biochemical composition	432:454	biochemical composition	432:454	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	0	23	theme	Lagoon	10:15	arg1	Effect					0:5	Effect	0:5	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.	0:122	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	0	24	theme	Water	25:29	arg1	Depth					31:35	Sea Water Depth	21:35	Sea Water Depth	21:35	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	4	25	dep	higher	689:694	arg1	p < 0.05					697:704	p < 0.05	697:704	p < 0.05	697:704	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	0	26	from	Growth	60:65	arg1	Northeast					102:110	the Northeast	98:110	the Northeast of Tunisia	98:121	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	3	27	dep	site	507:510	arg1	day-1					526:530	1.42 ± 0.65% day-1	513:530	1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively	513:588	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	0	28	theme	Sea	21:23	arg1	Depth					31:35	Sea Water Depth	21:35	Sea Water Depth	21:35	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	1	29	theme	Farnham	263:269	arg1	growth					149:154	the growth	145:154	the growth	145:154	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	29	theme	Farnham	263:269	arg1	composition					172:182	biochemical composition	160:182	biochemical composition	160:182	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	0	30	theme	Tunisia	115:121	arg1	Northeast					102:110	the Northeast	98:110	the Northeast of Tunisia	98:121	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	3	31	theme	1.42 ± 0.65	513:523	arg1	%					524:524	%	524:524	%	524:524	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	1	32	from	Lagoon	286:291	arg1	growth					149:154	the growth	145:154	the growth	145:154	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	32	from	Lagoon	286:291	arg1	composition					172:182	biochemical composition	160:182	biochemical composition	160:182	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	5	33	theme	depth	902:906	arg1	%					932:932	20.74 ± 7.22%	920:932	20.74 ± 7.22%	920:932	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	33	theme	depth	902:906	arg1	%					950:950	22.02 ± 6.34%	938:950	22.02 ± 6.34% for the BL and the BB	938:972	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	33	theme	depth	902:906	arg1	independent					874:884	independent	874:884	independent	874:884	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	33	theme	depth	902:906	arg1	contents					860:867	The proteins contents	847:867	The proteins contents	847:867	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	3	34	theme	%	524:524	arg1	day-1					526:530	1.42 ± 0.65% day-1	513:530	1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively	513:588	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	0	35	theme	Depth	31:35	arg1	Effect					0:5	Effect	0:5	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.	0:122	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	5	36	theme	culture	911:917	arg1	culture					911:917	culture	911:917	culture	911:917	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	36	theme	culture	911:917	arg1	depth					902:906	depth	902:906	depth	902:906	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	36	theme	culture	911:917	arg1	site					893:896	site	893:896	site	893:896	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	8	37	theme	G.	1282:1283	arg1	gracilis					1285:1292	G. gracilis	1282:1292	G. gracilis cultured in Bizerte Bay	1282:1316	Our results suggest that G. gracilis cultured in Bizerte Bay can be used in a cascading biorefinery approach.					
32572043	4	38	dep	degree	811:816	arg1	%					827:827	6 ± 2.00%	819:827	6 ± 2.00%	819:827	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	38	dep	degree	811:816	arg1	%					843:843	8.80 ± 0.86%	832:843	8.80 ± 0.86%	832:843	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	4	39	dep	lower	785:789	arg1	p < 0.05					792:799	p < 0.05	792:799	p < 0.05	792:799	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	8	40	theme	biorefinery	1345:1355	arg1	approach					1357:1364	a cascading biorefinery approach	1333:1364	a cascading biorefinery approach	1333:1364	Our results suggest that G. gracilis cultured in Bizerte Bay can be used in a cascading biorefinery approach.					
32572043	1	41	theme	Bizerte	278:284	arg1	BL					294:295	BL	294:295	BL	294:295	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	41	theme	Bizerte	278:284	arg1	Lagoon					286:291	the Bizerte Lagoon	274:291	the Bizerte Lagoon (BL)	274:296	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	4	42	theme	3,6-anhydrogalactose	707:726	arg1	contents					737:744	a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents	687:744	a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%)	687:777	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	5	43	dep	BL	960:961	arg1	the					956:958	the	956:958	the	956:958	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	8	44	theme	cascading	1335:1343	arg1	approach					1357:1364	a cascading biorefinery approach	1333:1364	a cascading biorefinery approach	1333:1364	Our results suggest that G. gracilis cultured in Bizerte Bay can be used in a cascading biorefinery approach.					
32572043	4	45	theme	lower	785:789	arg1	degree					811:816	a lower (p < 0.05) sulphate degree	783:816	a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%)	783:844	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	0	46	from	Composition	83:93	arg1	Northeast					102:110	the Northeast	98:110	the Northeast of Tunisia	98:121	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	6	47	dep	higher	1040:1045	arg1	p < 0.05					1048:1055	p < 0.05	1048:1055	p < 0.05	1048:1055	R-phycoerythrin (R-PE) contents were significantly higher (p < 0.05) in the BB (0.86 ± 0.31 mg g-1) than those obtained in the BL (0.33 ± 0.12 mg g-1).					
32572043	2	48	theme	alga	399:402	arg1	DGR					423:425	DGR	423:425	DGR	423:425	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	2	48	theme	alga	399:402	arg1	rate					417:420	alga daily growth rate	399:420	alga daily growth rate (DGR)	399:426	Effects of site and depth on alga daily growth rate (DGR) and biochemical composition were investigated.					
32572043	3	49	theme	%	547:547	arg1	day-1					549:553	1.19 ± 0.34% day-1	536:553	1.19 ± 0.34% day-1 for the BL	536:564	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	1	50	from	composition	172:182	arg1	BL					294:295	BL	294:295	BL	294:295	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	50	from	composition	172:182	arg1	Coast					332:336	Coast	332:336	Coast	332:336	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	50	from	composition	172:182	arg1	Lagoon					286:291	the Bizerte Lagoon	274:291	the Bizerte Lagoon (BL)	274:296	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	50	from	composition	172:182	arg1	Bay					310:312	Bizerte Bay	302:312	Bizerte Bay (BB)	302:317	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	50	from	composition	172:182	arg1	BB					315:316	BB	315:316	BB	315:316	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	3	51	dep	day-1	526:530	arg1	day-1					549:553	1.19 ± 0.34% day-1	536:553	1.19 ± 0.34% day-1 for the BL	536:564	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	3	51	dep	day-1	526:530	arg1	BB					574:575	the BB	570:575	the BB	570:575	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	0	52	theme	Gracilaria	40:49	arg1	Growth					60:65	Gracilaria gracilis Growth	40:65	Gracilaria gracilis Growth	40:65	Effect of Lagoon and Sea Water Depth on Gracilaria gracilis Growth and Biochemical Composition in the Northeast of Tunisia.					
32572043	3	53	theme	culture	499:505	arg1	site					507:510	culture site	499:510	culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively)	499:589	The DGR was affected by culture site (1.42 ± 0.65% day-1 and 1.19 ± 0.34% day-1 for the BL and the BB respectively).					
32572043	1	54	from	growth	149:154	arg1	BL					294:295	BL	294:295	BL	294:295	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	54	from	growth	149:154	arg1	Coast					332:336	Coast	332:336	Coast	332:336	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	54	from	growth	149:154	arg1	Lagoon					286:291	the Bizerte Lagoon	274:291	the Bizerte Lagoon (BL)	274:296	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	54	from	growth	149:154	arg1	Bay					310:312	Bizerte Bay	302:312	Bizerte Bay (BB)	302:317	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	1	54	from	growth	149:154	arg1	BB					315:316	BB	315:316	BB	315:316	This study evaluated the growth and biochemical composition of farming Gracilaria gracilis (Stackhouse) M. Steentoft, L. M. Irvine & W. F. Farnham in the Bizerte Lagoon (BL) and Bizerte Bay (BB) in the North Coast of Tunisia, using lantern nets.					
32572043	4	55	theme	sulphate	802:809	arg1	degree					811:816	a lower (p < 0.05) sulphate degree	783:816	a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%)	783:844	Agar yield, was higher (p < 0.05) in the BB than the BL (23.31 ± 2.64% vs. 19.19 ± 2.32%) with a higher (p < 0.05) 3,6-anhydrogalactose (3,6-AG) contents (41.37 ± 3.68% vs 23.30 ± 5.40%) and a lower (p < 0.05) sulphate degree (6 ± 2.00% vs 8.80 ± 0.86%).					
32572043	5	56	dep	independent	874:884	arg1	%					932:932	20.74 ± 7.22%	920:932	20.74 ± 7.22%	920:932	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	56	dep	independent	874:884	arg1	%					950:950	22.02 ± 6.34%	938:950	22.02 ± 6.34% for the BL and the BB	938:972	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	56	dep	independent	874:884	arg1	independent					874:884	independent	874:884	independent	874:884	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32572043	5	56	dep	independent	874:884	arg1	contents					860:867	The proteins contents	847:867	The proteins contents	847:867	The proteins contents were independent of the site and depth of culture (20.74 ± 7.22% and 22.02 ± 6.34% for the BL and the BB respectively).					
32331714	5	0	theme	ethyl	1108:1112	arg1	EL					1126:1127	EL	1126:1127	EL	1126:1127	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	0	theme	ethyl	1108:1112	arg1	levulinate					1114:1123	ethyl levulinate	1108:1123	ethyl levulinate (EL)	1108:1128	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	1	1	from	palm	223:226	arg1	reservoir					264:272	an excellent reservoir	251:272	an excellent reservoir of lignocellulosic material which full potential remains untapped	251:338	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	1	1	from	palm	223:226	arg1	Biomass					206:212	Biomass	206:212	Biomass from oil palm frond leaves (OPFL)	206:246	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	6	2	theme	structural	1410:1419	arg1	rigidification					1421:1434	the appreciable structural rigidification	1394:1434	the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase	1394:1542	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	1	3	theme	lignocellulosic	277:291	arg1	material					293:300	lignocellulosic material	277:300	lignocellulosic material	277:300	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	0	4	theme	alginate/nanocellulose/montmorillonite	79:116	arg1	composite					118:126	a ternary alginate/nanocellulose/montmorillonite composite	69:126	a ternary alginate/nanocellulose/montmorillonite composite	69:126	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	5	theme	atomic	704:709	arg1	micrographs					717:727	atomic force micrographs	704:727	atomic force micrographs	704:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	5	6	theme	protein	1053:1059	arg1	7 h					1011:1013	7 h	1011:1013	7 h of immobilization	1011:1031	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	6	theme	protein	1053:1059	arg1	loading					1061:1067	7 mg/mL protein loading	1045:1067	7 mg/mL protein loading	1045:1067	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	6	7	theme	appreciable	1398:1408	arg1	rigidification					1421:1434	the appreciable structural rigidification	1394:1434	the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase	1394:1542	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	1	8	theme	material	293:300	arg1	reservoir					264:272	an excellent reservoir	251:272	an excellent reservoir of lignocellulosic material which full potential remains untapped	251:338	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	1	8	theme	material	293:300	arg1	Biomass					206:212	Biomass	206:212	Biomass from oil palm frond leaves (OPFL)	206:246	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	0	9	theme	Physicochemical	129:143	arg1	characterization					145:160	Physicochemical characterization	129:160	Physicochemical characterization	129:160	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	10	theme	force	711:715	arg1	micrographs					717:727	atomic force micrographs	704:727	atomic force micrographs	704:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	5	11	theme	CRL	990:992	arg1	immobilization					972:985	The optimized Taguchi Design-assisted immobilization	934:985	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading)	934:1068	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	6	12	theme	thermal	1512:1518	arg1	stability					1520:1528	thermal stability	1512:1528	thermal stability	1512:1528	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	5	13	theme	[M	1183:1184	arg1	FTIR					1207:1210	FTIR	1207:1210	FTIR	1207:1210	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	13	theme	[M	1183:1184	arg1	data					1258:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	13	theme	[M	1183:1184	arg1	+m/z					1187:1190	gas chromatography-mass spectrometric ([M] +m/z 144	1144:1194	gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3)	1144:1204	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	6	14	theme	higher	1266:1271	arg1	50℃					1303:1305	50℃	1303:1305	50℃	1303:1305	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	14	theme	higher	1266:1271	arg1	temperature					1290:1300	A higher optimal reaction temperature	1264:1300	A higher optimal reaction temperature (50℃)	1264:1306	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	1	15	theme	full	308:311	arg1	potential					313:321	full potential	308:321	full potential	308:321	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	5	16	dep	ALG/NC/MMT	999:1008	arg1	7 h					1011:1013	7 h	1011:1013	7 h of immobilization	1011:1031	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	16	dep	ALG/NC/MMT	999:1008	arg1	loading					1061:1067	7 mg/mL protein loading	1045:1067	7 mg/mL protein loading	1045:1067	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	16	dep	ALG/NC/MMT	999:1008	arg1	pH					1039:1040	pH 5	1039:1042	pH 5	1039:1042	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	16	dep	ALG/NC/MMT	999:1008	arg1	35℃					1034:1036	35℃	1034:1036	35℃	1034:1036	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	1	17	theme	oil	219:221	arg1	OPFL					242:245	OPFL	242:245	OPFL	242:245	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	1	17	theme	oil	219:221	arg1	palm					223:226	oil palm frond leaves	219:239	oil palm frond leaves (OPFL)	219:246	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	3	18	from	emission	661:668	arg1	topology					585:592	The coarser topology	573:592	The coarser topology	573:592	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	18	from	emission	661:668	arg1	presence					602:609	the presence	598:609	the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs	598:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	5	19	theme	spectrometric	1168:1180	arg1	FTIR					1207:1210	FTIR	1207:1210	FTIR	1207:1210	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	19	theme	spectrometric	1168:1180	arg1	data					1258:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	19	theme	spectrometric	1168:1180	arg1	+m/z					1187:1190	gas chromatography-mass spectrometric ([M] +m/z 144	1144:1194	gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3)	1144:1204	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	4	20	dep	transform	852:860	arg1	infrared					862:869	infrared	862:869	transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT	852:931	In addition, amide peaks at 3478 and 1640 cm-1 in the fourier transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT.					
32331714	6	21	theme	catalytic	1489:1497	arg1	activity					1499:1506	catalytic activity	1489:1506	catalytic activity	1489:1506	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	0	22	theme	design-assisted	8:22	arg1	immobilization					24:37	Taguchi design-assisted immobilization	0:37	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.	0:204	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	5	23	theme	%	1103:1103	arg1	yield					1088:1092	a production yield	1075:1092	a production yield of 92.89 % of ethyl levulinate (EL)	1075:1128	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	24	theme	Taguchi	948:954	arg1	immobilization					972:985	The optimized Taguchi Design-assisted immobilization	934:985	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading)	934:1068	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	3	25	from	globules	639:646	arg1	micrographs					717:727	atomic force micrographs	704:727	atomic force micrographs	704:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	25	from	globules	639:646	arg1	emission					661:668	the field emission scanning electron micrographs and atomic force micrographs	651:727	emission	661:668	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	1	26	theme	frond	228:232	arg1	OPFL					242:245	OPFL	242:245	OPFL	242:245	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	1	26	theme	frond	228:232	arg1	palm					223:226	oil palm frond leaves	219:239	oil palm frond leaves (OPFL)	219:246	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	0	27	theme	Taguchi	0:6	arg1	immobilization					24:37	Taguchi design-assisted immobilization	0:37	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.	0:204	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	28	theme	CRL	771:773	arg1	existence					758:766	the existence	754:766	the existence of CRL	754:773	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	6	29	theme	optimal	1273:1279	arg1	50℃					1303:1305	50℃	1303:1305	50℃	1303:1305	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	29	theme	optimal	1273:1279	arg1	temperature					1290:1300	A higher optimal reaction temperature	1264:1300	A higher optimal reaction temperature (50℃)	1264:1306	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	3	30	theme	coarser	577:583	arg1	topology					585:592	The coarser topology	573:592	The coarser topology	573:592	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	31	from	topology	585:592	arg1	micrographs					717:727	atomic force micrographs	704:727	atomic force micrographs	704:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	31	from	topology	585:592	arg1	emission					661:668	the field emission scanning electron micrographs and atomic force micrographs	651:727	emission	661:668	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	2	32	theme	lipase	430:435	arg1	immobilization					397:410	the covalent immobilization	384:410	the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT)	384:570	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	4	33	dep	fourier	844:850	arg1	transform					852:860	transform	852:860	transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT	852:931	In addition, amide peaks at 3478 and 1640 cm-1 in the fourier transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT.					
32331714	6	34	theme	reaction	1281:1288	arg1	50℃					1303:1305	50℃	1303:1305	50℃	1303:1305	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	34	theme	reaction	1281:1288	arg1	temperature					1290:1300	A higher optimal reaction temperature	1264:1300	A higher optimal reaction temperature (50℃)	1264:1306	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	35	dep	activity	1499:1506	arg1	the					1485:1487	the	1485:1487	the	1485:1487	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	5	36	theme	Design-assisted	956:970	arg1	immobilization					972:985	The optimized Taguchi Design-assisted immobilization	934:985	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading)	934:1068	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	37	theme	gas	1144:1146	arg1	FTIR					1207:1210	FTIR	1207:1210	FTIR	1207:1210	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	37	theme	gas	1144:1146	arg1	data					1258:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	37	theme	gas	1144:1146	arg1	+m/z					1187:1190	gas chromatography-mass spectrometric ([M] +m/z 144	1144:1194	gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3)	1144:1204	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	0	38	theme	thermal	163:169	arg1	stability					171:179	thermal stability	163:179	thermal stability	163:179	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	39	theme	field	655:659	arg1	emission					661:668	the field emission scanning electron micrographs and atomic force micrographs	651:727	emission	661:668	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	2	40	theme	ternary	450:456	arg1	support					458:464	a ternary support	448:464	a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT)	448:570	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	0	41	theme	rugosa	50:55	arg1	lipase					57:62	Candida rugosa lipase	42:62	Candida rugosa lipase	42:62	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	5	42	theme	chromatography-mass	1148:1166	arg1	FTIR					1207:1210	FTIR	1207:1210	FTIR	1207:1210	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	42	theme	chromatography-mass	1148:1166	arg1	data					1258:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	42	theme	chromatography-mass	1148:1166	arg1	+m/z					1187:1190	gas chromatography-mass spectrometric ([M] +m/z 144	1144:1194	gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3)	1144:1204	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	0	43	theme	reusability	185:195	arg1	studies					197:203	reusability studies	185:203	reusability studies	185:203	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	44	from	presence	602:609	arg1	micrographs					717:727	atomic force micrographs	704:727	atomic force micrographs	704:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	44	from	presence	602:609	arg1	emission					661:668	the field emission scanning electron micrographs and atomic force micrographs	651:727	emission	661:668	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	0	45	theme	Candida	42:48	arg1	lipase					57:62	Candida rugosa lipase	42:62	Candida rugosa lipase	42:62	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	46	theme	globules	639:646	arg1	topology					585:592	The coarser topology	573:592	The coarser topology	573:592	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	46	theme	globules	639:646	arg1	presence					602:609	the presence	598:609	the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs	598:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	5	47	dep	+m/z	1187:1190	arg1	C7H12O3					1197:1203	C7H12O3	1197:1203	C7H12O3	1197:1203	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	6	48	theme	esterification	1358:1371	arg1	cycles					1373:1378	up to 9 esterification cycles	1350:1378	up to 9 esterification cycles	1350:1378	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	3	49	from	micrographs	717:727	arg1	topology					585:592	The coarser topology	573:592	The coarser topology	573:592	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	3	49	from	micrographs	717:727	arg1	presence					602:609	the presence	598:609	the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs	598:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	5	50	theme	production	1077:1086	arg1	yield					1088:1092	a production yield	1075:1092	a production yield of 92.89 % of ethyl levulinate (EL)	1075:1128	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	2	51	theme	covalent	388:395	arg1	immobilization					397:410	the covalent immobilization	384:410	the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT)	384:570	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	0	52	theme	lipase	57:62	arg1	immobilization					24:37	Taguchi design-assisted immobilization	0:37	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.	0:204	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	6	53	dep	9	1356:1356	arg1	to					1353:1354	to	1353:1354	to	1353:1354	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	1	54	dep	palm	223:226	arg1	leaves					234:239	leaves	234:239	leaves	234:239	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	5	55	theme	nuclear	1216:1222	arg1	CAS-539-88-8					1244:1255	CAS-539-88-8	1244:1255	CAS-539-88-8	1244:1255	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	55	theme	nuclear	1216:1222	arg1	resonance					1233:1241	nuclear magnetic resonance	1216:1241	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	56	theme	7 mg/mL	1045:1051	arg1	7 h					1011:1013	7 h	1011:1013	7 h of immobilization	1011:1031	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	56	theme	7 mg/mL	1045:1051	arg1	loading					1061:1067	7 mg/mL protein loading	1045:1067	7 mg/mL protein loading	1045:1067	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	3	57	theme	spherical	629:637	arg1	globules					639:646	characteristic spherical globules	614:646	characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs	614:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	6	58	theme	lipase	1537:1542	arg1	activity					1499:1506	catalytic activity	1489:1506	catalytic activity	1489:1506	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	58	theme	lipase	1537:1542	arg1	stability					1520:1528	thermal stability	1512:1528	thermal stability	1512:1528	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	5	59	theme	magnetic	1224:1231	arg1	CAS-539-88-8					1244:1255	CAS-539-88-8	1244:1255	CAS-539-88-8	1244:1255	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	59	theme	magnetic	1224:1231	arg1	resonance					1233:1241	nuclear magnetic resonance	1216:1241	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	60	theme	levulinate	1114:1123	arg1	EL					1126:1127	EL	1126:1127	EL	1126:1127	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	60	theme	levulinate	1114:1123	arg1	%					1103:1103	92.89 %	1097:1103	92.89 % of ethyl levulinate (EL)	1097:1128	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	60	theme	levulinate	1114:1123	arg1	levulinate					1114:1123	ethyl levulinate	1108:1123	ethyl levulinate (EL)	1108:1128	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	3	61	theme	scanning	670:677	arg1	micrographs					688:698	scanning electron micrographs	670:698	scanning electron micrographs	670:698	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	0	62	dep	immobilization	24:37	arg1	characterization					145:160	Physicochemical characterization	129:160	Physicochemical characterization	129:160	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	0	62	dep	immobilization	24:37	arg1	stability					171:179	thermal stability	163:179	thermal stability	163:179	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	0	62	dep	immobilization	24:37	arg1	studies					197:203	reusability studies	185:203	reusability studies	185:203	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	5	63	theme	optimized	938:946	arg1	immobilization					972:985	The optimized Taguchi Design-assisted immobilization	934:985	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading)	934:1068	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	64	theme	resonance	1233:1241	arg1	data					1258:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	nuclear magnetic resonance (CAS-539-88-8) data	1216:1261	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	64	theme	resonance	1233:1241	arg1	+m/z					1187:1190	gas chromatography-mass spectrometric ([M] +m/z 144	1144:1194	gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3)	1144:1204	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	1	65	theme	excellent	254:262	arg1	reservoir					264:272	an excellent reservoir	251:272	an excellent reservoir of lignocellulosic material which full potential remains untapped	251:338	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	1	65	theme	excellent	254:262	arg1	Biomass					206:212	Biomass	206:212	Biomass from oil palm frond leaves (OPFL)	206:246	Biomass from oil palm frond leaves (OPFL) is an excellent reservoir of lignocellulosic material which full potential remains untapped.					
32331714	0	66	theme	ternary	71:77	arg1	composite					118:126	a ternary alginate/nanocellulose/montmorillonite composite	69:126	a ternary alginate/nanocellulose/montmorillonite composite	69:126	Taguchi design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies.					
32331714	3	67	theme	electron	679:686	arg1	micrographs					688:698	scanning electron micrographs	670:698	scanning electron micrographs	670:698	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	5	68	theme	immobilization	1018:1031	arg1	7 h					1011:1013	7 h	1011:1013	7 h of immobilization	1011:1031	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	68	theme	immobilization	1018:1031	arg1	loading					1061:1067	7 mg/mL protein loading	1045:1067	7 mg/mL protein loading	1045:1067	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	68	theme	immobilization	1018:1031	arg1	pH					1039:1040	pH 5	1039:1042	pH 5	1039:1042	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	5	68	theme	immobilization	1018:1031	arg1	35℃					1034:1036	35℃	1034:1036	35℃	1034:1036	The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89 % of ethyl levulinate (EL), as proven by gas chromatography-mass spectrometric ([M] +m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data.					
32331714	2	69	theme	rugosa	423:428	arg1	CRL					438:440	CRL	438:440	CRL	438:440	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	2	69	theme	rugosa	423:428	arg1	lipase					430:435	Candida rugosa lipase	415:435	Candida rugosa lipase (CRL)	415:441	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	6	70	theme	biocatalyst	1443:1453	arg1	rigidification					1421:1434	the appreciable structural rigidification	1394:1434	the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase	1394:1542	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	3	71	theme	characteristic	614:627	arg1	globules					639:646	characteristic spherical globules	614:646	characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs	614:727	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32331714	6	72	theme	CRL-ALG/NC/MMT	1331:1344	arg1	50℃					1303:1305	50℃	1303:1305	50℃	1303:1305	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	72	theme	CRL-ALG/NC/MMT	1331:1344	arg1	temperature					1290:1300	A higher optimal reaction temperature	1264:1300	A higher optimal reaction temperature (50℃)	1264:1306	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	6	72	theme	CRL-ALG/NC/MMT	1331:1344	arg1	reusability					1316:1326	the reusability	1312:1326	the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles	1312:1378	A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.					
32331714	2	73	theme	Candida	415:421	arg1	CRL					438:440	CRL	438:440	CRL	438:440	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	2	73	theme	Candida	415:421	arg1	lipase					430:435	Candida rugosa lipase	415:435	Candida rugosa lipase (CRL)	415:441	This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT).					
32331714	3	74	dep	emission	661:668	arg1	micrographs					688:698	scanning electron micrographs	670:698	scanning electron micrographs	670:698	The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT.					
32805290	1	0	theme	152+154Eu	204:212	arg1	behaviors					191:199	behaviors	191:199	behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	191:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	2	1	theme	nanotubes	607:615	arg1	surface					558:564	the surface	554:564	the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation	554:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	4	2	theme	removal	795:801	arg1	capacity					803:810	high removal capacity	790:810	high removal capacity of 321.77, 369.91, and 456.46 mg/g	790:845	The composite shows high removal capacity of 321.77, 369.91, and 456.46 mg/g towards 152+154Eu, 60Co, and 134Cs radionuclides, respectively.					
32805290	1	3	theme	composite	359:367	arg1	Removal					183:189	Removal	183:189	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite	183:367	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	0	4	theme	152+154Eu	142:150	arg1	removal					131:137	removal	131:137	removal of 152+154Eu, 60Co and 134Cs radionuclides	131:180	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	2	5	theme	gamma	638:642	arg1	radiation					644:652	gamma radiation	638:652	gamma radiation	638:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	1	6	theme	60Co	215:218	arg1	behaviors					191:199	behaviors	191:199	behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	191:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	0	7	theme	60Co	153:156	arg1	removal					131:137	removal	131:137	removal of 152+154Eu, 60Co and 134Cs radionuclides	131:180	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	2	8	theme	carbon	600:605	arg1	CTS/o-MWCNTs					618:629	CTS/o-MWCNTs	618:629	CTS/o-MWCNTs	618:629	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	2	8	theme	carbon	600:605	arg1	nanotubes					607:615	chitosan/oxidized multi-walled carbon nanotubes	569:615	chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation	569:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	0	9	dep	152+154Eu	142:150	arg1	radionuclides					168:180	radionuclides	168:180	radionuclides	168:180	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	0	10	theme	radiation-induced	6:22	arg1	preparation					24:34	Gamma radiation-induced preparation	0:34	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	0:105	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	2	11	theme	CTS-AA-VP/o-MWCNTs	422:439	arg1	composite					441:449	CTS-AA-VP/o-MWCNTs composite	422:449	CTS-AA-VP/o-MWCNTs composite	422:449	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	0	12	theme	Gamma	0:4	arg1	preparation					24:34	Gamma radiation-induced preparation	0:34	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	0:105	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	1	13	theme	134Cs	225:229	arg1	radionuclides					231:243	134Cs radionuclides	225:243	134Cs radionuclides	225:243	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	1	14	theme	radionuclides	231:243	arg1	behaviors					191:199	behaviors	191:199	behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	191:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	0	15	theme	134Cs	162:166	arg1	removal					131:137	removal	131:137	removal of 152+154Eu, 60Co and 134Cs radionuclides	131:180	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	4	16	theme	456.46 mg/g	835:845	arg1	capacity					803:810	high removal capacity	790:810	high removal capacity of 321.77, 369.91, and 456.46 mg/g	790:845	The composite shows high removal capacity of 321.77, 369.91, and 456.46 mg/g towards 152+154Eu, 60Co, and 134Cs radionuclides, respectively.					
32805290	4	17	theme	high	790:793	arg1	capacity					803:810	high removal capacity	790:810	high removal capacity of 321.77, 369.91, and 456.46 mg/g	790:845	The composite shows high removal capacity of 321.77, 369.91, and 456.46 mg/g towards 152+154Eu, 60Co, and 134Cs radionuclides, respectively.					
32805290	0	18	theme	acid-1-vinyl-2-vinylpyrrolidone/multiwalled	56:98	arg1	carbon					100:105	chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	39:105	chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	39:105	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	1	19	theme	batch	394:398	arg1	technique					411:419	batch adsorption technique	394:419	batch adsorption technique	394:419	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	2	20	theme	1-vinyl-2-vinylpyrrolidone	517:542	arg1	copolymerization					475:490	copolymerization	475:490	copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation	475:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	2	21	theme	acid	503:506	arg1	copolymerization					475:490	copolymerization	475:490	copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation	475:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	1	22	theme	Chitosan-acrylic	250:265	arg1	nanotubes					328:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	0	23	theme	chitosan-acrylic	39:54	arg1	carbon					100:105	chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	39:105	chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	39:105	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	1	24	theme	adsorption	400:409	arg1	technique					411:419	batch adsorption technique	394:419	batch adsorption technique	394:419	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	2	25	theme	multi-walled	587:598	arg1	CTS/o-MWCNTs					618:629	CTS/o-MWCNTs	618:629	CTS/o-MWCNTs	618:629	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	2	25	theme	multi-walled	587:598	arg1	nanotubes					607:615	chitosan/oxidized multi-walled carbon nanotubes	569:615	chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation	569:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	1	26	theme	acid-1-vinyl-2-vinylpyrrolidone/oxidized	267:306	arg1	nanotubes					328:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	2	27	theme	chitosan/oxidized	569:585	arg1	CTS/o-MWCNTs					618:629	CTS/o-MWCNTs	618:629	CTS/o-MWCNTs	618:629	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	2	27	theme	chitosan/oxidized	569:585	arg1	nanotubes					607:615	chitosan/oxidized multi-walled carbon nanotubes	569:615	chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation	569:652	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	4	28	theme	321.77	815:820	arg1	capacity					803:810	high removal capacity	790:810	high removal capacity of 321.77, 369.91, and 456.46 mg/g	790:845	The composite shows high removal capacity of 321.77, 369.91, and 456.46 mg/g towards 152+154Eu, 60Co, and 134Cs radionuclides, respectively.					
32805290	0	29	theme	carbon	100:105	arg1	preparation					24:34	Gamma radiation-induced preparation	0:34	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon	0:105	Gamma radiation-induced preparation of chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/multiwalled carbon nanotubes composite for removal of 152+154Eu, 60Co and 134Cs radionuclides.					
32805290	1	30	dep	composite	359:367	arg1	behaviors					191:199	behaviors	191:199	behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	191:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	4	31	theme	134Cs	876:880	arg1	radionuclides					882:894	134Cs radionuclides	876:894	134Cs radionuclides	876:894	The composite shows high removal capacity of 321.77, 369.91, and 456.46 mg/g towards 152+154Eu, 60Co, and 134Cs radionuclides, respectively.					
32805290	4	32	theme	369.91	823:828	arg1	capacity					803:810	high removal capacity	790:810	high removal capacity of 321.77, 369.91, and 456.46 mg/g	790:845	The composite shows high removal capacity of 321.77, 369.91, and 456.46 mg/g towards 152+154Eu, 60Co, and 134Cs radionuclides, respectively.					
32805290	1	33	theme	multi-walled	308:319	arg1	nanotubes					328:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	3	34	theme	composite	759:767	arg1	morphology					707:716	morphology	707:716	morphology	707:716	SEM, TGA, and FTIR were applied to characterize the morphology, thermal stability, and structure of the composite.					
32805290	3	34	theme	composite	759:767	arg1	stability					727:735	thermal stability	719:735	thermal stability	719:735	SEM, TGA, and FTIR were applied to characterize the morphology, thermal stability, and structure of the composite.					
32805290	3	34	theme	composite	759:767	arg1	structure					742:750	structure	742:750	structure	742:750	SEM, TGA, and FTIR were applied to characterize the morphology, thermal stability, and structure of the composite.					
32805290	1	35	theme	carbon	321:326	arg1	nanotubes					328:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes	250:336	Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique.					
32805290	2	36	theme	acrylic	495:501	arg1	AA					509:510	AA	509:510	AA	509:510	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	2	36	theme	acrylic	495:501	arg1	acid					503:506	acrylic acid	495:506	acrylic acid (AA)	495:511	CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic acid (AA) and 1-vinyl-2-vinylpyrrolidone (VP) onto the surface of chitosan/oxidized multi-walled carbon nanotubes (CTS/o-MWCNTs) using gamma radiation.					
32805290	3	37	theme	thermal	719:725	arg1	stability					727:735	thermal stability	719:735	thermal stability	719:735	SEM, TGA, and FTIR were applied to characterize the morphology, thermal stability, and structure of the composite.					
32070518	2	0	theme	composite	499:507	arg1	system					509:514	the fiber-reinforced composite system	478:514	the fiber-reinforced composite system between crosslinked PNIPAm and CNFs	478:550	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	1	1	theme	nanofibrils	150:160	arg1	composite					178:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	2	2	theme	fiber-reinforced	482:497	arg1	system					509:514	the fiber-reinforced composite system	478:514	the fiber-reinforced composite system between crosslinked PNIPAm and CNFs	478:550	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	4	3	theme	switchable	713:722	arg1	bioadhesivity					724:736	switchable bioadhesivity	713:736	switchable bioadhesivity to bacteria	713:748	It was found that PNIPAm/CNF hydrogels exhibit switchable bioadhesivity to bacteria in response to CNF distribution in the hydrogels.					
32070518	3	4	theme	optical	557:563	arg1	tunability					565:574	The optical tunability	553:574	The optical tunability	553:574	The optical tunability was found to be correlated to the micro/nano structures of the PNIPAm/CNF hydrogel films.					
32070518	1	5	theme	new	258:260	arg1	platform					262:269	a new platform	256:269	a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties	256:389	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	6	6	theme	PNIPAm/CNF	982:991	arg1	hydrogels					993:1001	The prepared PNIPAm/CNF hydrogels	969:1001	The prepared PNIPAm/CNF hydrogels	969:1001	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	5	7	theme	remarkable	856:865	arg1	reduction					871:879	a remarkable 8°C reduction	854:879	a remarkable 8°C reduction of the LCST	854:891	After 2.0 wt% CNF was incorporated, it was found that a remarkable 8°C reduction of the LCST was achieved relative to PNIPAm hydrogel crosslinked by TEGDMA without CNF.					
32070518	1	8	theme	optical	356:362	arg1	properties					380:389	thus tuning optical and bioadhesive properties	344:389	thus tuning optical and bioadhesive properties	344:389	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	4	9	theme	PNIPAm/CNF	684:693	arg1	hydrogels					695:703	PNIPAm/CNF hydrogels	684:703	PNIPAm/CNF hydrogels	684:703	It was found that PNIPAm/CNF hydrogels exhibit switchable bioadhesivity to bacteria in response to CNF distribution in the hydrogels.					
32070518	1	10	theme	CNFs	163:166	arg1	composite					178:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	2	11	theme	crosslinked	524:534	arg1	PNIPAm					536:541	crosslinked PNIPAm	524:541	crosslinked PNIPAm	524:541	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	6	12	theme	temperature-sensitive	1121:1141	arg1	them					1085:1088	them	1085:1088	them	1085:1088	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	12	theme	temperature-sensitive	1121:1141	arg1	sensors					1143:1149	durable temperature-sensitive sensors	1113:1149	durable temperature-sensitive sensors	1113:1149	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	5	13	theme	8°C	867:869	arg1	reduction					871:879	a remarkable 8°C reduction	854:879	a remarkable 8°C reduction of the LCST	854:891	After 2.0 wt% CNF was incorporated, it was found that a remarkable 8°C reduction of the LCST was achieved relative to PNIPAm hydrogel crosslinked by TEGDMA without CNF.					
32070518	0	14	theme	tunable	7:13	arg1	bioadhesion					15:25	Highly tunable bioadhesion	0:25	Highly tunable bioadhesion	0:25	Highly tunable bioadhesion and optics of 3D printable PNIPAm/cellulose nanofibrils hydrogels.					
32070518	1	15	theme	bioadhesive	368:378	arg1	properties					380:389	thus tuning optical and bioadhesive properties	344:389	thus tuning optical and bioadhesive properties	344:389	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	4	16	from	distribution	769:780	arg1	hydrogels					789:797	the hydrogels	785:797	the hydrogels	785:797	It was found that PNIPAm/CNF hydrogels exhibit switchable bioadhesivity to bacteria in response to CNF distribution in the hydrogels.					
32070518	4	17	theme	CNF	765:767	arg1	distribution					769:780	CNF distribution	765:780	CNF distribution in the hydrogels	765:797	It was found that PNIPAm/CNF hydrogels exhibit switchable bioadhesivity to bacteria in response to CNF distribution in the hydrogels.					
32070518	1	18	theme	hydrogel	169:176	arg1	composite					178:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	6	19	theme	prepared	973:980	arg1	hydrogels					993:1001	The prepared PNIPAm/CNF hydrogels	969:1001	The prepared PNIPAm/CNF hydrogels	969:1001	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	1	20	theme	hybrid	96:101	arg1	N-isopropylacrylamide					108:128	N-isopropylacrylamide	108:128	N-isopropylacrylamide	108:128	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	1	20	theme	hybrid	96:101	arg1	PNIPAm					132:137	PNIPAm	132:137	PNIPAm	132:137	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	1	20	theme	hybrid	96:101	arg1	poly					103:106	hybrid poly	96:106	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	2	21	theme	PNIPAm/CNF	436:445	arg1	hydrogels					447:455	the as-printed PNIPAm/CNF hydrogels	421:455	the as-printed PNIPAm/CNF hydrogels	421:455	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	1	22	theme	poly	103:106	arg1	composite					178:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	2	23	theme	as-printed	425:434	arg1	hydrogels					447:455	the as-printed PNIPAm/CNF hydrogels	421:455	the as-printed PNIPAm/CNF hydrogels	421:455	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	1	24	theme	lower	286:290	arg1	properties					329:338	lower critical solution temperature (LCST) properties	286:338	lower critical solution temperature (LCST) properties	286:338	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	6	25	used	used	1105:1108	arg2	sensors					1143:1149	durable temperature-sensitive sensors	1113:1149	durable temperature-sensitive sensors	1113:1149	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	25	used	used	1105:1108	arg2	them					1085:1088	them	1085:1088	them	1085:1088	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	25	used	used	1105:1108	arg2	devices					1177:1183	functional biomedical devices	1155:1183	functional biomedical devices	1155:1183	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	26	theme	functional	1155:1164	arg1	devices					1177:1183	functional biomedical devices	1155:1183	functional biomedical devices	1155:1183	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	26	theme	functional	1155:1164	arg1	them					1085:1088	them	1085:1088	them	1085:1088	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	27	theme	thermal	1057:1063	arg1	performance					1065:1075	thermal performance	1057:1075	thermal performance	1057:1075	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	1	28	theme	critical	292:299	arg1	properties					329:338	lower critical solution temperature (LCST) properties	286:338	lower critical solution temperature (LCST) properties	286:338	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	2	29	theme	interest	409:416	arg1	phenomena					396:404	The phenomena	392:404	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels	392:455	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	5	30	theme	wt	810:811	arg1	CNF					814:816	2.0 wt% CNF	806:816	2.0 wt% CNF	806:816	After 2.0 wt% CNF was incorporated, it was found that a remarkable 8°C reduction of the LCST was achieved relative to PNIPAm hydrogel crosslinked by TEGDMA without CNF.					
32070518	1	31	theme	solution	301:308	arg1	properties					329:338	lower critical solution temperature (LCST) properties	286:338	lower critical solution temperature (LCST) properties	286:338	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	3	32	theme	hydrogel	650:657	arg1	films					659:663	the PNIPAm/CNF hydrogel films	635:663	the PNIPAm/CNF hydrogel films	635:663	The optical tunability was found to be correlated to the micro/nano structures of the PNIPAm/CNF hydrogel films.					
32070518	3	33	theme	micro/nano	610:619	arg1	structures					621:630	the micro/nano structures	606:630	the micro/nano structures of the PNIPAm/CNF hydrogel films	606:663	The optical tunability was found to be correlated to the micro/nano structures of the PNIPAm/CNF hydrogel films.					
32070518	1	34	theme	inverted	206:213	arg1	printing					236:243	inverted stereolithography 3D printing	206:243	inverted stereolithography 3D printing	206:243	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	0	35	theme	printable	44:52	arg1	nanofibrils					71:81	3D printable PNIPAm/cellulose nanofibrils	41:81	3D printable PNIPAm/cellulose nanofibrils	41:81	Highly tunable bioadhesion and optics of 3D printable PNIPAm/cellulose nanofibrils hydrogels.					
32070518	1	36	theme	temperature	310:320	arg1	properties					329:338	lower critical solution temperature (LCST) properties	286:338	lower critical solution temperature (LCST) properties	286:338	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	3	37	theme	films	659:663	arg1	structures					621:630	the micro/nano structures	606:630	the micro/nano structures of the PNIPAm/CNF hydrogel films	606:663	The optical tunability was found to be correlated to the micro/nano structures of the PNIPAm/CNF hydrogel films.					
32070518	0	38	theme	3D	41:42	arg1	nanofibrils					71:81	3D printable PNIPAm/cellulose nanofibrils	41:81	3D printable PNIPAm/cellulose nanofibrils	41:81	Highly tunable bioadhesion and optics of 3D printable PNIPAm/cellulose nanofibrils hydrogels.					
32070518	6	39	theme	biomedical	1166:1175	arg1	devices					1177:1183	functional biomedical devices	1155:1183	functional biomedical devices	1155:1183	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	39	theme	biomedical	1166:1175	arg1	them					1085:1088	them	1085:1088	them	1085:1088	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	2	40	from	phenomena	396:404	arg1	hydrogels					447:455	the as-printed PNIPAm/CNF hydrogels	421:455	the as-printed PNIPAm/CNF hydrogels	421:455	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	4	41	dep	distribution	769:780	arg1	response					753:760	response	753:760	response	753:760	It was found that PNIPAm/CNF hydrogels exhibit switchable bioadhesivity to bacteria in response to CNF distribution in the hydrogels.					
32070518	1	42	theme	LCST	323:326	arg1	properties					329:338	lower critical solution temperature (LCST) properties	286:338	lower critical solution temperature (LCST) properties	286:338	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	5	43	theme	%	812:812	arg1	CNF					814:816	2.0 wt% CNF	806:816	2.0 wt% CNF	806:816	After 2.0 wt% CNF was incorporated, it was found that a remarkable 8°C reduction of the LCST was achieved relative to PNIPAm hydrogel crosslinked by TEGDMA without CNF.					
32070518	5	44	theme	PNIPAm	918:923	arg1	hydrogel					925:932	PNIPAm hydrogel	918:932	PNIPAm hydrogel crosslinked by TEGDMA without CNF	918:966	After 2.0 wt% CNF was incorporated, it was found that a remarkable 8°C reduction of the LCST was achieved relative to PNIPAm hydrogel crosslinked by TEGDMA without CNF.					
32070518	6	45	contain	possessed	1003:1011	arg2	bioadhesion					1040:1050	bioadhesion	1040:1050	bioadhesion	1040:1050	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	45	contain	possessed	1003:1011	arg2	optical					1031:1037	optical	1031:1037	optical	1031:1037	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	45	contain	possessed	1003:1011	arg2	performance					1065:1075	thermal performance	1057:1075	thermal performance	1057:1075	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	45	contain	possessed	1003:1011	arg1	hydrogels					993:1001	The prepared PNIPAm/CNF hydrogels	969:1001	The prepared PNIPAm/CNF hydrogels	969:1001	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	1	46	theme	stereolithography	215:231	arg1	printing					236:243	inverted stereolithography 3D printing	206:243	inverted stereolithography 3D printing	206:243	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	3	47	theme	PNIPAm/CNF	639:648	arg1	films					659:663	the PNIPAm/CNF hydrogel films	635:663	the PNIPAm/CNF hydrogel films	635:663	The optical tunability was found to be correlated to the micro/nano structures of the PNIPAm/CNF hydrogel films.					
32070518	5	48	theme	LCST	888:891	arg1	reduction					871:879	a remarkable 8°C reduction	854:879	a remarkable 8°C reduction of the LCST	854:891	After 2.0 wt% CNF was incorporated, it was found that a remarkable 8°C reduction of the LCST was achieved relative to PNIPAm hydrogel crosslinked by TEGDMA without CNF.					
32070518	1	49	theme	3D	233:234	arg1	printing					236:243	inverted stereolithography 3D printing	206:243	inverted stereolithography 3D printing	206:243	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	0	50	theme	nanofibrils	71:81	arg1	bioadhesion					15:25	Highly tunable bioadhesion	0:25	Highly tunable bioadhesion	0:25	Highly tunable bioadhesion and optics of 3D printable PNIPAm/cellulose nanofibrils hydrogels.					
32070518	0	50	theme	nanofibrils	71:81	arg1	optics					31:36	optics	31:36	optics	31:36	Highly tunable bioadhesion and optics of 3D printable PNIPAm/cellulose nanofibrils hydrogels.					
32070518	2	51	link	crosslinked	524:534	arg1	PNIPAm					536:541	crosslinked PNIPAm	524:541	crosslinked PNIPAm	524:541	The phenomena of interest in the as-printed PNIPAm/CNF hydrogels may be attributed to the fiber-reinforced composite system between crosslinked PNIPAm and CNFs.					
32070518	0	52	theme	PNIPAm/cellulose	54:69	arg1	nanofibrils					71:81	3D printable PNIPAm/cellulose nanofibrils	41:81	3D printable PNIPAm/cellulose nanofibrils	41:81	Highly tunable bioadhesion and optics of 3D printable PNIPAm/cellulose nanofibrils hydrogels.					
32070518	6	53	theme	durable	1113:1119	arg1	them					1085:1088	them	1085:1088	them	1085:1088	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	6	53	theme	durable	1113:1119	arg1	sensors					1143:1149	durable temperature-sensitive sensors	1113:1149	durable temperature-sensitive sensors	1113:1149	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
32070518	1	54	theme	/cellulose	139:148	arg1	composite					178:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite	94:186	A hybrid poly(N-isopropylacrylamide) (PNIPAm)/cellulose nanofibrils (CNFs) hydrogel composite was fabricated by inverted stereolithography 3D printing to provide a new platform for regulating lower critical solution temperature (LCST) properties and thus tuning optical and bioadhesive properties.					
32070518	6	55	theme	reversible	1020:1029	arg1	optical					1031:1037	optical	1031:1037	optical	1031:1037	The prepared PNIPAm/CNF hydrogels possessed highly reversible optical, bioadhesion, and thermal performance, making them suitable to be used as durable temperature-sensitive sensors and functional biomedical devices.					
34250923	8	0	theme	MS	1086:1087	arg1	scaffold					1089:1096	the SF/CS/TGF-β1-loaded MS scaffold	1062:1096	the SF/CS/TGF-β1-loaded MS scaffold	1062:1096	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	8	1	theme	SF/CS/TGF-β1-loaded	1066:1084	arg1	scaffold					1089:1096	the SF/CS/TGF-β1-loaded MS scaffold	1062:1096	the SF/CS/TGF-β1-loaded MS scaffold	1062:1096	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	7	2	theme	synthetic	995:1003	arg1	activity					1005:1012	chondrocyte synthetic activity	983:1012	chondrocyte synthetic activity	983:1012	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	7	3	theme	good	904:907	arg1	release					916:922	good TGF-β1 release	904:922	good TGF-β1 release	904:922	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	9	4	located	observed	1264:1271	arg1	rats					1276:1279	rats	1276:1279	rats	1276:1279	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	9	4	located	observed	1264:1271	arg2	response					1251:1258	a low inflammatory response	1232:1258	a low inflammatory response	1232:1258	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	5	5	theme	adsorption	729:738	arg1	method					740:745	the adsorption method	725:745	the adsorption method	725:745	Then, SF/CS/TGF-β1-loaded MS scaffolds were prepared by the adsorption method.					
34250923	9	6	theme	inflammatory	1238:1249	arg1	response					1251:1258	a low inflammatory response	1232:1258	a low inflammatory response	1232:1258	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	1	7	theme	BACKGROUND	84:93	arg1	TGF-β1					126:131	TGF-β1	126:131	TGF-β1	126:131	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	1	7	theme	BACKGROUND	84:93	arg1	factor-β1					115:123	BACKGROUND Transforming growth factor-β1	84:123	BACKGROUND Transforming growth factor-β1 (TGF-β1)	84:132	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	7	8	theme	TGF-β1	909:914	arg1	release					916:922	good TGF-β1 release	904:922	good TGF-β1 release	904:922	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	8	9	theme	cell	1024:1027	arg1	experiments					1043:1053	In vitro cell proliferation experiments	1015:1053	In vitro cell proliferation experiments	1015:1053	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	1	10	theme	Transforming	95:106	arg1	TGF-β1					126:131	TGF-β1	126:131	TGF-β1	126:131	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	1	10	theme	Transforming	95:106	arg1	factor-β1					115:123	BACKGROUND Transforming growth factor-β1	84:123	BACKGROUND Transforming growth factor-β1 (TGF-β1)	84:132	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	8	11	theme	In	1015:1016	arg1	experiments					1043:1053	In vitro cell proliferation experiments	1015:1053	In vitro cell proliferation experiments	1015:1053	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	9	12	dep	in	1200:1201	arg1	vivo					1203:1206	vivo	1203:1206	vivo	1203:1206	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	3	13	dep	/chitosan	385:393	arg1	CS					396:397	CS	396:397	CS	396:397	OBJECTIVE In this study, a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs) was created for cartilage reparation.					
34250923	6	14	theme	in	817:818	arg1	implantation					825:836	in vivo implantation	817:836	in vivo implantation	817:836	TGF-β1 release capacity, degradation patterns, cytocompatibility and in vivo implantation were evaluted.					
34250923	1	15	theme	growth	108:113	arg1	TGF-β1					126:131	TGF-β1	126:131	TGF-β1	126:131	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	1	15	theme	growth	108:113	arg1	factor-β1					115:123	BACKGROUND Transforming growth factor-β1	84:123	BACKGROUND Transforming growth factor-β1 (TGF-β1)	84:132	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	8	16	dep	showed	1055:1060	arg1	promote					1104:1110	promote	1104:1110	could promote chondrocytes adhesion, growth, proliferation	1098:1155	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	8	16	dep	showed	1055:1060	arg1	maintained					1161:1170	maintained	1161:1170	maintained the cellular morphology	1161:1194	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	9	17	theme	low	1234:1236	arg1	response					1251:1258	a low inflammatory response	1232:1258	a low inflammatory response	1232:1258	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	7	18	theme	SF/CS/TGF-β1-loaded	865:883	arg1	scaffold					888:895	The SF/CS/TGF-β1-loaded MS scaffold	861:895	The SF/CS/TGF-β1-loaded MS scaffold	861:895	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	3	19	theme	TGF-β1-loaded	427:439	arg1	MSs					455:457	MSs	455:457	MSs	455:457	OBJECTIVE In this study, a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs) was created for cartilage reparation.					
34250923	3	19	theme	TGF-β1-loaded	427:439	arg1	microspheres					441:452	TGF-β1-loaded microspheres	427:452	TGF-β1-loaded microspheres (MSs)	427:458	OBJECTIVE In this study, a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs) was created for cartilage reparation.					
34250923	4	20	theme	scaffold	551:558	arg1	proportion					517:526	The optimal proportion	505:526	The optimal proportion of the SF/CS composite scaffold	505:558	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	8	21	theme	proliferation	1029:1041	arg1	experiments					1043:1053	In vitro cell proliferation experiments	1015:1053	In vitro cell proliferation experiments	1015:1053	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	7	22	theme	MS	885:886	arg1	scaffold					888:895	The SF/CS/TGF-β1-loaded MS scaffold	861:895	The SF/CS/TGF-β1-loaded MS scaffold	861:895	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	1	23	theme	extracellular	201:213	arg1	ECM					223:225	ECM	223:225	ECM	223:225	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	1	23	theme	extracellular	201:213	arg1	matrix					215:220	extracellular matrix	201:220	extracellular matrix (ECM)	201:226	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	4	24	theme	composite	541:549	arg1	scaffold					551:558	the SF/CS composite scaffold	531:558	the SF/CS composite scaffold	531:558	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	8	25	theme	chondrocytes	1112:1123	arg1	proliferation					1143:1155	proliferation	1143:1155	proliferation	1143:1155	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	8	25	theme	chondrocytes	1112:1123	arg1	growth					1135:1140	growth	1135:1140	growth	1135:1140	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	8	25	theme	chondrocytes	1112:1123	arg1	adhesion					1125:1132	chondrocytes adhesion	1112:1132	chondrocytes adhesion	1112:1132	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	1	26	theme	matrix	215:220	arg1	growth					173:178	chondrocyte growth	161:178	chondrocyte growth	161:178	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	1	26	theme	matrix	215:220	arg1	synthesis					188:196	the synthesis	184:196	the synthesis of extracellular matrix (ECM)	184:226	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	4	27	theme	SF/CS	535:539	arg1	scaffold					551:558	the SF/CS composite scaffold	531:558	the SF/CS composite scaffold	531:558	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	7	28	theme	chondrocyte	983:993	arg1	activity					1005:1012	chondrocyte synthetic activity	983:1012	chondrocyte synthetic activity	983:1012	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	2	29	theme	increasing	311:320	arg1	interest					322:329	increasing interest	311:329	increasing interest	311:329	Due to the rapid metabolism, controlled release systems for TGF-β1 have attracted increasing interest recently.					
34250923	6	30	dep	in	817:818	arg1	vivo					820:823	vivo	820:823	vivo	820:823	TGF-β1 release capacity, degradation patterns, cytocompatibility and in vivo implantation were evaluted.					
34250923	0	31	theme	microsphere	36:46	arg1	scaffolds					48:56	microsphere scaffolds	36:56	microsphere scaffolds	36:56	Silk fibroin/chitosan/TGF-β1-loaded microsphere scaffolds for cartilage reparation.					
34250923	3	32	dep	OBJECTIVE	341:349	arg1	created					464:470	created	464:470	was created for cartilage reparation	460:495	OBJECTIVE In this study, a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs) was created for cartilage reparation.					
34250923	8	33	dep	In	1015:1016	arg1	vitro					1018:1022	vitro	1018:1022	vitro	1018:1022	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	0	34	theme	cartilage	62:70	arg1	reparation					72:81	cartilage reparation	62:81	cartilage reparation	62:81	Silk fibroin/chitosan/TGF-β1-loaded microsphere scaffolds for cartilage reparation.					
34250923	3	35	theme	cartilage	476:484	arg1	reparation					486:495	cartilage reparation	476:495	cartilage reparation	476:495	OBJECTIVE In this study, a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs) was created for cartilage reparation.					
34250923	2	36	theme	rapid	240:244	arg1	metabolism					246:255	the rapid metabolism	236:255	the rapid metabolism	236:255	Due to the rapid metabolism, controlled release systems for TGF-β1 have attracted increasing interest recently.					
34250923	6	37	theme	degradation	773:783	arg1	patterns					785:792	degradation patterns	773:792	degradation patterns	773:792	TGF-β1 release capacity, degradation patterns, cytocompatibility and in vivo implantation were evaluted.					
34250923	4	38	theme	fibroblasts	641:651	arg1	rate					633:636	the proliferation rate	615:636	the proliferation rate of fibroblasts	615:651	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	4	38	theme	fibroblasts	641:651	arg1	micromorphology					595:609	their micromorphology	589:609	their micromorphology	589:609	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	3	39	theme	/chitosan	385:393	arg1	scaffold					400:407	a silk fibroin (SF)/chitosan (CS) scaffold	366:407	a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs)	366:458	OBJECTIVE In this study, a silk fibroin (SF)/chitosan (CS) scaffold incorporated with TGF-β1-loaded microspheres (MSs) was created for cartilage reparation.					
34250923	4	40	theme	optimal	509:515	arg1	proportion					517:526	The optimal proportion	505:526	The optimal proportion of the SF/CS composite scaffold	505:558	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	10	41	theme	promising	1424:1432	arg1	option					1446:1451	a promising therapeutic option	1422:1451	a promising therapeutic option for cartilage reparation	1422:1476	CONCLUSION the results indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation.					
34250923	10	42	theme	SF/CS/TGF-β1-loaded	1379:1397	arg1	scaffold					1402:1409	our SF/CS/TGF-β1-loaded MS scaffold	1375:1409	our SF/CS/TGF-β1-loaded MS scaffold	1375:1409	CONCLUSION the results indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation.					
34250923	5	43	theme	SF/CS/TGF-β1-loaded	675:693	arg1	scaffolds					698:706	SF/CS/TGF-β1-loaded MS scaffolds	675:706	SF/CS/TGF-β1-loaded MS scaffolds	675:706	Then, SF/CS/TGF-β1-loaded MS scaffolds were prepared by the adsorption method.					
34250923	9	44	theme	good	1314:1317	arg1	biocompatibility					1319:1334	good biocompatibility	1314:1334	good biocompatibility	1314:1334	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	9	45	theme	in	1200:1201	arg1	study					1208:1212	An in vivo study	1197:1212	An in vivo study	1197:1212	An in vivo study demonstrated that a low inflammatory response was observed in rats and that the materials exhibited good biocompatibility.					
34250923	1	46	theme	important	143:151	arg1	role					153:156	an important role	140:156	an important role	140:156	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	5	47	theme	MS	695:696	arg1	scaffolds					698:706	SF/CS/TGF-β1-loaded MS scaffolds	675:706	SF/CS/TGF-β1-loaded MS scaffolds	675:706	Then, SF/CS/TGF-β1-loaded MS scaffolds were prepared by the adsorption method.					
34250923	2	48	theme	release	269:275	arg1	systems					277:283	controlled release systems	258:283	controlled release systems for TGF-β1	258:294	Due to the rapid metabolism, controlled release systems for TGF-β1 have attracted increasing interest recently.					
34250923	6	49	theme	release	755:761	arg1	capacity					763:770	TGF-β1 release capacity	748:770	TGF-β1 release capacity	748:770	TGF-β1 release capacity, degradation patterns, cytocompatibility and in vivo implantation were evaluted.					
34250923	8	50	theme	cellular	1176:1183	arg1	morphology					1185:1194	the cellular morphology	1172:1194	the cellular morphology	1172:1194	In vitro cell proliferation experiments showed the SF/CS/TGF-β1-loaded MS scaffold could promote chondrocytes adhesion, growth, proliferation and maintained the cellular morphology.					
34250923	2	51	theme	controlled	258:267	arg1	systems					277:283	controlled release systems	258:283	controlled release systems for TGF-β1	258:294	Due to the rapid metabolism, controlled release systems for TGF-β1 have attracted increasing interest recently.					
34250923	6	52	theme	TGF-β1	748:753	arg1	capacity					763:770	TGF-β1 release capacity	748:770	TGF-β1 release capacity	748:770	TGF-β1 release capacity, degradation patterns, cytocompatibility and in vivo implantation were evaluted.					
34250923	10	53	theme	MS	1399:1400	arg1	scaffold					1402:1409	our SF/CS/TGF-β1-loaded MS scaffold	1375:1409	our SF/CS/TGF-β1-loaded MS scaffold	1375:1409	CONCLUSION the results indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation.					
34250923	10	54	theme	therapeutic	1434:1444	arg1	option					1446:1451	a promising therapeutic option	1422:1451	a promising therapeutic option for cartilage reparation	1422:1476	CONCLUSION the results indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation.					
34250923	10	55	theme	cartilage	1457:1465	arg1	reparation					1467:1476	cartilage reparation	1457:1476	cartilage reparation	1457:1476	CONCLUSION the results indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation.					
34250923	1	56	theme	chondrocyte	161:171	arg1	growth					173:178	chondrocyte growth	161:178	chondrocyte growth	161:178	BACKGROUND Transforming growth factor-β1 (TGF-β1) plays an important role in chondrocyte growth and the synthesis of extracellular matrix (ECM).					
34250923	4	57	theme	proliferation	619:631	arg1	rate					633:636	the proliferation rate	615:636	the proliferation rate of fibroblasts	615:651	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
34250923	10	58	dep	CONCLUSION	1337:1346	arg1	indicated					1360:1368	indicated	1360:1368	indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation	1360:1476	CONCLUSION the results indicated that our SF/CS/TGF-β1-loaded MS scaffold constitute a promising therapeutic option for cartilage reparation.					
34250923	7	59	dep	RESULTS	853:859	arg1	showed					897:902	showed	897:902	showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity	897:1012	RESULTS The SF/CS/TGF-β1-loaded MS scaffold showed good TGF-β1 release over more than 16 days, which could sequentially stimulate chondrocyte synthetic activity.					
34250923	4	60	dep	METHOD	498:503	arg1	determined					564:573	determined	564:573	was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface	560:666	METHOD The optimal proportion of the SF/CS composite scaffold was determined by evaluating their micromorphology and the proliferation rate of fibroblasts on the surface.					
32758606	5	0	theme	adsorption	1006:1015	arg1	data					1017:1020	the adsorption data	1002:1020	the adsorption data fitted well	1002:1032	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	5	1	theme	pseudo-second	972:984	arg1	model					992:996	a pseudo-second order model	970:996	a pseudo-second order model	970:996	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	4	2	dep	attained	832:839	arg1	VI					807:808	VI	807:808	VI	807:808	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	8	3	used	used	1390:1393	arg2	composite					1371:1379	the synthesized polymer composite	1347:1379	the synthesized polymer composite	1347:1379	Adsorption-desorption experiments revealed that the synthesized polymer composite could be used for up to four consecutive cycles.					
32758606	5	4	theme	isotherm	1047:1054	arg1	model					1056:1060	the Langmuir isotherm model	1034:1060	the Langmuir isotherm model	1034:1060	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	0	5	theme	selective	73:81	arg1	removal					83:89	the selective removal	69:89	the selective removal of hexavalent chromium from waste water	69:129	Polypyrrole-coated gum ghatti-grafted poly(acrylamide) composite for the selective removal of hexavalent chromium from waste water.					
32758606	5	6	dep	model	992:996	arg1	model					1056:1060	the Langmuir isotherm model	1034:1060	the Langmuir isotherm model	1034:1060	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	1	7	theme	pyrrole	418:424	arg1	monomers					426:433	the pyrrole monomers	414:433	the pyrrole monomers	414:433	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	1	8	theme	study	148:152	arg1	aim					136:138	The aim	132:138	The aim of this study	132:152	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	1	9	theme	multi-step	234:243	arg1	method					268:273	a multi-step aqueous polymerization method	232:273	a multi-step aqueous polymerization method	232:273	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	0	10	theme	hexavalent	94:103	arg1	chromium					105:112	hexavalent chromium	94:112	hexavalent chromium	94:112	Polypyrrole-coated gum ghatti-grafted poly(acrylamide) composite for the selective removal of hexavalent chromium from waste water.					
32758606	3	11	theme	contact	591:597	arg1	time					599:602	contact time	591:602	contact time	591:602	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	6	12	theme	maximum	1067:1073	arg1	321.5					1156:1160	321.5	1156:1160	321.5	1156:1160	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	6	12	theme	maximum	1067:1073	arg1	capacity					1086:1093	The maximum adsorption capacity	1063:1093	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K	1063:1133	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	1	13	theme	monomers	426:433	arg1	polymerization					396:409	the polymerization	392:409	the polymerization of the pyrrole monomers	392:433	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	4	14	theme	Cr	877:878	arg1	pH 2.0					844:849	pH 2.0	844:849	pH 2.0	844:849	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	4	14	theme	Cr	877:878	arg1	concentration					860:872	initial concentration	852:872	initial concentration of Cr(VI) of 100 mg/L	852:894	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	4	14	theme	Cr	877:878	arg1	dosage					911:916	adsorbent dosage	901:916	adsorbent dosage of 0.035 g	901:927	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	1	15	theme	aqueous	245:251	arg1	method					268:273	a multi-step aqueous polymerization method	232:273	a multi-step aqueous polymerization method	232:273	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	3	16	from	effects	556:562	arg1	performance					664:674	the adsorption performance	649:674	the adsorption performance of the composite	649:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	17	theme	Cr	622:623	arg1	time					599:602	contact time	591:602	contact time	591:602	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	17	theme	Cr	622:623	arg1	temperature					634:644	temperature	634:644	temperature	634:644	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	17	theme	Cr	622:623	arg1	concentration					605:617	concentration	605:617	concentration of Cr(VI)	605:627	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	17	theme	Cr	622:623	arg1	pH					571:572	the pH	567:572	the pH	567:572	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	17	theme	Cr	622:623	arg1	dose					585:588	adsorbent dose	575:588	adsorbent dose	575:588	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	4	18	theme	0.035 g	921:927	arg1	pH 2.0					844:849	pH 2.0	844:849	pH 2.0	844:849	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	4	18	theme	0.035 g	921:927	arg1	concentration					860:872	initial concentration	852:872	initial concentration of Cr(VI) of 100 mg/L	852:894	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	4	18	theme	0.035 g	921:927	arg1	dosage					911:916	adsorbent dosage	901:916	adsorbent dosage of 0.035 g	901:927	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	1	19	theme	polymerization	253:266	arg1	method					268:273	a multi-step aqueous polymerization method	232:273	a multi-step aqueous polymerization method	232:273	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	7	20	theme	ions	1223:1226	arg1	presence					1200:1207	The presence	1196:1207	The presence of coexisting ions in the solution	1196:1242	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	0	21	theme	chromium	105:112	arg1	removal					83:89	the selective removal	69:89	the selective removal of hexavalent chromium from waste water	69:129	Polypyrrole-coated gum ghatti-grafted poly(acrylamide) composite for the selective removal of hexavalent chromium from waste water.					
32758606	1	22	theme	gum	342:344	arg1	copolymer					370:378	the gum ghatti-acrylamide graft copolymer	338:378	the gum ghatti-acrylamide graft copolymer	338:378	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	6	23	from	308	1120:1122	arg1	321.5					1156:1160	321.5	1156:1160	321.5	1156:1160	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	6	23	from	308	1120:1122	arg1	capacity					1086:1093	The maximum adsorption capacity	1063:1093	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K	1063:1133	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	3	24	theme	composite	683:691	arg1	performance					664:674	the adsorption performance	649:674	the adsorption performance of the composite	649:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	25	dep	Cr	622:623	arg1	VI					625:626	VI	625:626	VI	625:626	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	4	26	dep	Cr	745:746	arg1	VI					748:749	VI	748:749	VI	748:749	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	1	27	theme	ghatti-acrylamide	346:362	arg1	copolymer					370:378	the gum ghatti-acrylamide graft copolymer	338:378	the gum ghatti-acrylamide graft copolymer	338:378	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	4	28	theme	Cr	745:746	arg1	adsorption					731:740	The adsorption	727:740	The adsorption of Cr(VI) on the composite	727:767	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	0	29	theme	Polypyrrole-coated	0:17	arg1	gum					19:21	Polypyrrole-coated gum	0:21	Polypyrrole-coated gum	0:21	Polypyrrole-coated gum ghatti-grafted poly(acrylamide) composite for the selective removal of hexavalent chromium from waste water.					
32758606	7	30	dep	Cr	1277:1278	arg1	VI					1280:1281	VI	1280:1281	VI	1280:1281	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	6	31	theme	adsorption	1075:1084	arg1	321.5					1156:1160	321.5	1156:1160	321.5	1156:1160	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	6	31	theme	adsorption	1075:1084	arg1	capacity					1086:1093	The maximum adsorption capacity	1063:1093	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K	1063:1133	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	4	32	theme	initial	852:858	arg1	concentration					860:872	initial concentration	852:872	initial concentration of Cr(VI) of 100 mg/L	852:894	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	6	33	from	298	1115:1117	arg1	321.5					1156:1160	321.5	1156:1160	321.5	1156:1160	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	6	33	from	298	1115:1117	arg1	capacity					1086:1093	The maximum adsorption capacity	1063:1093	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K	1063:1133	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	1	34	dep	absorbed	309:316	arg1	followed					380:387	followed	380:387	followed by the polymerization of the pyrrole monomers	380:433	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	0	35	theme	waste	119:123	arg1	water					125:129	waste water	119:129	waste water	119:129	Polypyrrole-coated gum ghatti-grafted poly(acrylamide) composite for the selective removal of hexavalent chromium from waste water.					
32758606	1	36	theme	graft	364:368	arg1	copolymer					370:378	the gum ghatti-acrylamide graft copolymer	338:378	the gum ghatti-acrylamide graft copolymer	338:378	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	4	37	from	adsorption	731:740	arg1	composite					759:767	the composite	755:767	the composite	755:767	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	8	38	theme	Adsorption-desorption	1299:1319	arg1	experiments					1321:1331	Adsorption-desorption experiments	1299:1331	Adsorption-desorption experiments	1299:1331	Adsorption-desorption experiments revealed that the synthesized polymer composite could be used for up to four consecutive cycles.					
32758606	4	39	theme	%	826:826	arg1	removal					811:817	removal	811:817	removal of 99.6%	811:826	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	5	40	theme	order	986:990	arg1	model					992:996	a pseudo-second order model	970:996	a pseudo-second order model	970:996	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	1	41	theme	copolymer	370:378	arg1	network					327:333	the network	323:333	the network of the gum ghatti-acrylamide graft copolymer	323:378	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	1	42	dep	synthesize	161:170	arg1	VI					469:470	VI	469:470	VI	469:470	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	4	43	theme	adsorbent	901:909	arg1	dosage					911:916	adsorbent dosage	901:916	adsorbent dosage of 0.035 g	901:927	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	1	44	theme	pyrrole	289:295	arg1	monomer					297:303	the pyrrole monomer	285:303	the pyrrole monomer	285:303	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	7	45	theme	Cr	1277:1278	arg1	adsorption					1263:1272	the adsorption	1259:1272	the adsorption of Cr(VI)	1259:1282	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	3	46	theme	concentration	605:617	arg1	effects					556:562	The effects	552:562	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite	552:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	3	47	theme	temperature	634:644	arg1	effects					556:562	The effects	552:562	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite	552:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	6	48	theme	adsorbent	1102:1110	arg1	321.5					1156:1160	321.5	1156:1160	321.5	1156:1160	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	6	48	theme	adsorbent	1102:1110	arg1	capacity					1086:1093	The maximum adsorption capacity	1063:1093	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K	1063:1133	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	3	49	theme	adsorbent	575:583	arg1	dose					585:588	adsorbent dose	575:588	adsorbent dose	575:588	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	1	50	theme	Cr	466:467	arg1	removal					455:461	the removal	451:461	the removal of Cr	451:467	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	5	51	theme	adsorption	950:959	arg1	kinetics					934:941	The kinetics	930:941	The kinetics of the adsorption	930:959	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	3	52	theme	dose	585:588	arg1	effects					556:562	The effects	552:562	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite	552:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	4	53	dep	Cr	877:878	arg1	VI					880:881	VI	880:881	VI	880:881	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	8	54	theme	polymer	1363:1369	arg1	composite					1371:1379	the synthesized polymer composite	1347:1379	the synthesized polymer composite	1347:1379	Adsorption-desorption experiments revealed that the synthesized polymer composite could be used for up to four consecutive cycles.					
32758606	3	55	theme	pH	571:572	arg1	effects					556:562	The effects	552:562	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite	552:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	6	56	from	318 K	1129:1133	arg1	321.5					1156:1160	321.5	1156:1160	321.5	1156:1160	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	6	56	from	318 K	1129:1133	arg1	capacity					1086:1093	The maximum adsorption capacity	1063:1093	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K	1063:1133	The maximum adsorption capacity of the adsorbent at 298, 308, and 318 K was determined to be 321.5, 357, and 416 mg/g, respectively.					
32758606	7	57	attach	presence	1200:1207	arg1	solution					1235:1242	the solution	1231:1242	the solution	1231:1242	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	7	57	attach	presence	1200:1207	arg2	ions					1223:1226	coexisting ions	1212:1226	coexisting ions	1212:1226	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	4	58	theme	100 mg/L	887:894	arg1	Cr					877:878	Cr	877:878	Cr(VI) of 100 mg/L	877:894	The adsorption of Cr(VI) on the composite depended strongly on the pH, and a Cr(VI) removal of 99.6% was attained at pH 2.0, initial concentration of Cr(VI) of 100 mg/L, and adsorbent dosage of 0.035 g.					
32758606	1	59	theme	/polypyrrole	203:214	arg1	composite					216:224	a gum ghatti-g-poly(acrylamide)/polypyrrole composite	172:224	a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers	172:433	The aim of this study was to synthesize a gum ghatti-g-poly(acrylamide)/polypyrrole composite using a multi-step aqueous polymerization method, in which the pyrrole monomer was absorbed into the network of the gum ghatti-acrylamide graft copolymer followed by the polymerization of the pyrrole monomers, and use it for the removal of Cr(VI) from water.					
32758606	7	60	theme	coexisting	1212:1221	arg1	ions					1223:1226	coexisting ions	1212:1226	coexisting ions	1212:1226	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	8	61	theme	consecutive	1410:1420	arg1	cycles					1422:1427	up to four consecutive cycles	1399:1427	up to four consecutive cycles	1399:1427	Adsorption-desorption experiments revealed that the synthesized polymer composite could be used for up to four consecutive cycles.					
32758606	0	62	from	water	125:129	arg1	removal					83:89	the selective removal	69:89	the selective removal of hexavalent chromium from waste water	69:129	Polypyrrole-coated gum ghatti-grafted poly(acrylamide) composite for the selective removal of hexavalent chromium from waste water.					
32758606	3	63	theme	time	599:602	arg1	effects					556:562	The effects	552:562	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite	552:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
32758606	8	64	theme	synthesized	1351:1361	arg1	composite					1371:1379	the synthesized polymer composite	1347:1379	the synthesized polymer composite	1347:1379	Adsorption-desorption experiments revealed that the synthesized polymer composite could be used for up to four consecutive cycles.					
32758606	8	65	dep	four	1405:1408	arg1	to					1402:1403	to	1402:1403	to	1402:1403	Adsorption-desorption experiments revealed that the synthesized polymer composite could be used for up to four consecutive cycles.					
32758606	7	66	from	presence	1200:1207	arg1	solution					1235:1242	the solution	1231:1242	the solution	1231:1242	The presence of coexisting ions in the solution did not affect the adsorption of Cr(VI) significantly.					
32758606	5	67	theme	Langmuir	1038:1045	arg1	model					1056:1060	the Langmuir isotherm model	1034:1060	the Langmuir isotherm model	1034:1060	The kinetics of the adsorption followed a pseudo-second order model and the adsorption data fitted well the Langmuir isotherm model.					
32758606	3	68	theme	adsorption	653:662	arg1	performance					664:674	the adsorption performance	649:674	the adsorption performance of the composite	649:691	The effects of the pH, adsorbent dose, contact time, concentration of Cr(VI), and temperature on the adsorption performance of the composite were investigated systematically.					
33527746	0	0	theme	wound	86:90	arg1	technique					101:109	a three-dimensional printed wound dressing technique	58:109	a three-dimensional printed wound dressing technique	58:109	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	0	0	theme	wound	86:90	arg1	chitosan					46:53	chitosan	46:53	chitosan	46:53	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	8	1	theme	acid	1158:1161	arg1	nanocomposite					1163:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	1	theme	acid	1158:1161	arg1	scaffold					1206:1213	a promising wound healing scaffold	1180:1213	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	5	2	theme	Cu-CDs	635:640	arg1	concentration					642:654	Cu-CDs concentration	635:654	Cu-CDs concentration	635:654	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	3	3	theme	hydrogen	445:452	arg1	citrate					454:460	ammonium hydrogen citrate	436:460	ammonium hydrogen citrate	436:460	Cu-CDs were synthesized using ammonium hydrogen citrate under hydrothermal conditions.					
33527746	0	4	theme	printed	78:84	arg1	technique					101:109	a three-dimensional printed wound dressing technique	58:109	a three-dimensional printed wound dressing technique	58:109	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	0	4	theme	printed	78:84	arg1	chitosan					46:53	chitosan	46:53	chitosan	46:53	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	5	5	theme	concentration	642:654	arg1	0.25 mg ml-1					619:630	0.25 mg ml-1	619:630	0.25 mg ml-1 of Cu-CDs concentration	619:654	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	6	6	theme	rats	807:810	arg1	groups					797:802	groups	797:802	groups of rats	797:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	6	6	theme	rats	807:810	arg1	rats					807:810	rats	807:810	rats	807:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	7	7	theme	PDGF	1062:1065	arg1	expression					1048:1057	the relative expression	1035:1057	the relative expression of PDGF, TGF-β, and MMP-1 genes	1035:1089	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	7	7	theme	PDGF	1062:1065	arg1	analysis					1022:1029	histological analysis	1009:1029	histological analysis	1009:1029	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	6	8	dep	in	760:761	arg1	vivo					763:766	vivo	763:766	vivo	763:766	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	8	9	theme	promising	1182:1190	arg1	nanocomposite					1163:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	9	theme	promising	1182:1190	arg1	scaffold					1206:1213	a promising wound healing scaffold	1180:1213	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	6	10	theme	healing	774:780	arg1	experiments					782:792	in vivo wound healing experiments	760:792	in vivo wound healing experiments on groups of rats	760:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	1	11	theme	poly-lactic	254:264	arg1	PLA					272:274	PLA	272:274	PLA	272:274	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	1	11	theme	poly-lactic	254:264	arg1	acid					266:269	poly-lactic acid	254:269	poly-lactic acid (PLA)	254:275	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	0	12	theme	dressing	92:99	arg1	technique					101:109	a three-dimensional printed wound dressing technique	58:109	a three-dimensional printed wound dressing technique	58:109	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	0	12	theme	dressing	92:99	arg1	chitosan					46:53	chitosan	46:53	chitosan	46:53	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	6	13	theme	composite	881:889	arg1	scaffold					891:898	composite scaffold	881:898	composite scaffold	881:898	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	4	14	contain	containing	505:514	arg1	Formulation					493:503	Formulation	493:503	Formulation containing 1 mg ml-1 concentration of Cu-CDs	493:548	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
33527746	4	14	contain	containing	505:514	arg2	concentration					526:538	1 mg ml-1 concentration	516:538	1 mg ml-1 concentration of Cu-CDs	516:548	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
33527746	4	15	theme	excellent	560:568	arg1	activity					584:591	an excellent antibacterial activity	557:591	an excellent antibacterial activity against gram bacteria	557:613	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
33527746	5	16	theme	L929	732:735	arg1	cells					753:757	L929 fibroblast stem cells	732:757	L929 fibroblast stem cells	732:757	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	7	17	theme	MMP-1	1079:1083	arg1	genes					1085:1089	MMP-1 genes	1079:1089	MMP-1 genes	1079:1089	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	8	18	theme	CU-CDS/PLA/HA/chitosan/rosmarinic	1124:1156	arg1	nanocomposite					1163:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	18	theme	CU-CDS/PLA/HA/chitosan/rosmarinic	1124:1156	arg1	scaffold					1206:1213	a promising wound healing scaffold	1180:1213	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	2	19	theme	dots	351:354	arg1	Cu-CDs					357:362	copper carbon dots (Cu-CDs)	337:363	copper carbon dots (Cu-CDs)	337:363	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	5	20	theme	fibroblast	737:746	arg1	cells					753:757	L929 fibroblast stem cells	732:757	L929 fibroblast stem cells	732:757	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	7	21	theme	genes	1085:1089	arg1	expression					1048:1057	the relative expression	1035:1057	the relative expression of PDGF, TGF-β, and MMP-1 genes	1035:1089	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	7	21	theme	genes	1085:1089	arg1	analysis					1022:1029	histological analysis	1009:1029	histological analysis	1009:1029	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	2	22	theme	carbon	344:349	arg1	Cu-CDs					357:362	copper carbon dots (Cu-CDs)	337:363	copper carbon dots (Cu-CDs)	337:363	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	6	23	theme	optimal	858:864	arg1	formulation					866:876	the optimal formulation	854:876	the optimal formulation of composite scaffold	854:898	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	5	24	theme	stem	748:751	arg1	cells					753:757	L929 fibroblast stem cells	732:757	L929 fibroblast stem cells	732:757	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	2	25	theme	scaffold	290:297	arg1	core					282:285	The core	278:285	The core of scaffold	278:297	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	2	26	theme	copper	337:342	arg1	Cu-CDs					357:362	copper carbon dots (Cu-CDs)	337:363	copper carbon dots (Cu-CDs)	337:363	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	8	27	theme	wound	1192:1196	arg1	healing					1198:1204	wound healing	1192:1204	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	6	28	theme	wound	927:931	arg1	healing					933:939	the wound healing	923:939	the wound healing process	923:947	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	6	29	theme	wound	768:772	arg1	healing					774:780	in vivo wound healing	760:780	in vivo wound healing experiments on groups of rats	760:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	1	30	theme	three-dimensional	172:188	arg1	healing					209:215	a highly porous three-dimensional (3D)-printed wound healing	156:215	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	0	31	theme	novel	15:19	arg1	nanocomposite					21:33	a novel nanocomposite	13:33	a novel nanocomposite	13:33	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	4	32	theme	1 mg ml-1	516:524	arg1	concentration					526:538	1 mg ml-1 concentration	516:538	1 mg ml-1 concentration of Cu-CDs	516:548	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
33527746	0	33	theme	gene	124:127	arg1	expression					129:138	gene expression	124:138	gene expression	124:138	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	6	34	theme	scaffold	891:898	arg1	formulation					866:876	the optimal formulation	854:876	the optimal formulation of composite scaffold	854:898	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	2	35	theme	rosmarinic	366:375	arg1	acid					377:380	rosmarinic acid	366:380	rosmarinic acid	366:380	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	4	36	theme	Cu-CDs	543:548	arg1	concentration					526:538	1 mg ml-1 concentration	516:538	1 mg ml-1 concentration of Cu-CDs	516:548	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
33527746	7	37	theme	TGF-β	1068:1072	arg1	expression					1048:1057	the relative expression	1035:1057	the relative expression of PDGF, TGF-β, and MMP-1 genes	1035:1089	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	7	37	theme	TGF-β	1068:1072	arg1	analysis					1022:1029	histological analysis	1009:1029	histological analysis	1009:1029	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	6	38	from	experiments	782:792	arg1	groups					797:802	groups	797:802	groups of rats	797:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	6	38	from	experiments	782:792	arg1	rats					807:810	rats	807:810	rats	807:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	0	39	theme	nanocomposite	21:33	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a novel nanocomposite	0:33	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	6	40	dep	treatment	843:851	arg1	15 days					832:838	15 days	832:838	15 days	832:838	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	5	41	theme	cytotoxicity	710:721	arg1	assay					723:727	cytotoxicity assay	710:727	cytotoxicity assay on L929 fibroblast stem cells	710:757	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	5	42	from	assay	723:727	arg1	cells					753:757	L929 fibroblast stem cells	732:757	L929 fibroblast stem cells	732:757	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	1	43	dep	three-dimensional	172:188	arg1	3D					191:192	3D	191:192	3D	191:192	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	6	44	theme	in	760:761	arg1	healing					774:780	in vivo wound healing	760:780	in vivo wound healing experiments on groups of rats	760:810	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	1	45	theme	-printed	194:201	arg1	healing					209:215	a highly porous three-dimensional (3D)-printed wound healing	156:215	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	8	46	theme	skin	1255:1258	arg1	regeneration					1260:1271	skin regeneration	1255:1271	skin regeneration	1255:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	7	47	theme	relative	1039:1046	arg1	expression					1048:1057	the relative expression	1035:1057	the relative expression of PDGF, TGF-β, and MMP-1 genes	1035:1089	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	6	48	theme	PLA	965:967	arg1	scaffold					969:976	the PLA scaffold	961:976	the PLA scaffold	961:976	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	0	49	dep	containing	35:44	arg1	Emphasis					112:119	Emphasis	112:119	Emphasis on gene expression	112:138	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	8	50	theme	antibacterial	1110:1122	arg1	nanocomposite					1163:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	50	theme	antibacterial	1110:1122	arg1	scaffold					1206:1213	a promising wound healing scaffold	1180:1213	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	2	51	theme	hyaluronic	315:324	arg1	HA					332:333	HA	332:333	HA	332:333	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	2	51	theme	hyaluronic	315:324	arg1	acid					326:329	hyaluronic acid	315:329	hyaluronic acid (HA)	315:334	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	2	52	theme	chitosan	387:394	arg1	hydrogel					396:403	chitosan hydrogel	387:403	chitosan hydrogel	387:403	The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel.					
33527746	6	53	theme	healing	933:939	arg1	process					941:947	the wound healing process	923:947	the wound healing process	923:947	in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold.					
33527746	0	54	from	Emphasis	112:119	arg1	expression					129:138	gene expression	124:138	gene expression	124:138	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	3	55	theme	ammonium	436:443	arg1	citrate					454:460	ammonium hydrogen citrate	436:460	ammonium hydrogen citrate	436:460	Cu-CDs were synthesized using ammonium hydrogen citrate under hydrothermal conditions.					
33527746	5	56	contain	had	666:668	arg2	biocompatibility					677:692	a good biocompatibility	670:692	a good biocompatibility	670:692	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	5	56	contain	had	666:668	arg1	scaffold					657:664	scaffold	657:664	scaffold	657:664	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	5	57	theme	good	672:675	arg1	biocompatibility					677:692	a good biocompatibility	670:692	a good biocompatibility	670:692	At 0.25 mg ml-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells.					
33527746	1	58	theme	wound	203:207	arg1	healing					209:215	a highly porous three-dimensional (3D)-printed wound healing	156:215	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	8	59	theme	biocompatible	1096:1108	arg1	nanocomposite					1163:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	59	theme	biocompatible	1096:1108	arg1	scaffold					1206:1213	a promising wound healing scaffold	1180:1213	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	7	60	theme	histological	1009:1020	arg1	analysis					1022:1029	histological analysis	1009:1029	histological analysis	1009:1029	This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes.					
33527746	1	61	theme	healing	209:215	arg1	scaffold					228:235	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	0	62	theme	three-dimensional	60:76	arg1	technique					101:109	a three-dimensional printed wound dressing technique	58:109	a three-dimensional printed wound dressing technique	58:109	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	0	62	theme	three-dimensional	60:76	arg1	chitosan					46:53	chitosan	46:53	chitosan	46:53	Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression.					
33527746	8	63	theme	healing	1198:1204	arg1	nanocomposite					1163:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite	1092:1175	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	63	theme	healing	1198:1204	arg1	scaffold					1206:1213	a promising wound healing scaffold	1180:1213	a promising wound healing scaffold which highly accelerates the process of skin regeneration	1180:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	8	64	theme	regeneration	1260:1271	arg1	process					1244:1250	the process	1240:1250	the process of skin regeneration	1240:1271	The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.					
33527746	1	65	theme	core/shell	217:226	arg1	scaffold					228:235	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold	156:235	In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA).					
33527746	4	66	theme	antibacterial	570:582	arg1	activity					584:591	an excellent antibacterial activity	557:591	an excellent antibacterial activity against gram bacteria	557:613	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
33527746	3	67	theme	hydrothermal	468:479	arg1	conditions					481:490	hydrothermal conditions	468:490	hydrothermal conditions	468:490	Cu-CDs were synthesized using ammonium hydrogen citrate under hydrothermal conditions.					
33527746	4	68	theme	gram	601:604	arg1	bacteria					606:613	gram bacteria	601:613	gram bacteria	601:613	Formulation containing 1 mg ml-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria.					
34742448	4	0	theme	LMH	706:708	arg1	h-1					710:712	40 LMH h-1	703:712	40 LMH h-1	703:712	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	0	theme	LMH	706:708	arg1	permeance					692:700	superior water permeance	677:700	superior water permeance (40 LMH h-1)	677:713	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	3	1	theme	ZIF-8	573:577	arg1	suspension					550:559	the aqueous suspension	538:559	the aqueous suspension of ChNF and ZIF-8	538:577	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	6	2	theme	simple	950:955	arg1	strategy					957:964	a simple strategy	948:964	a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability	948:1123	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	3	3	theme	ChNF	564:567	arg1	suspension					550:559	the aqueous suspension	538:559	the aqueous suspension of ChNF and ZIF-8	538:577	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	5	4	theme	membranes	838:846	arg1	cut-off					813:819	cut-off	813:819	cut-off	813:819	Moreover, the molecular size cut-off of the composite membranes for dyes can be controlled in the range of less than 1 nm to 2 nm.					
34742448	3	5	theme	pore-size	505:513	arg1	control					515:521	pore-size control	505:521	pore-size control	505:521	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	6	6	theme	membranes	1007:1015	arg1	preparation					974:984	the preparation	970:984	the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability	970:1123	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	3	7	theme	aqueous	542:548	arg1	suspension					550:559	the aqueous suspension	538:559	the aqueous suspension of ChNF and ZIF-8	538:577	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	5	8	theme	less	891:894	arg1	range					882:886	the range	878:886	the range of less than 1 nm to 2 nm	878:912	Moreover, the molecular size cut-off of the composite membranes for dyes can be controlled in the range of less than 1 nm to 2 nm.					
34742448	4	9	theme	water	686:690	arg1	h-1					710:712	40 LMH h-1	703:712	40 LMH h-1	703:712	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	9	theme	water	686:690	arg1	permeance					692:700	superior water permeance	677:700	superior water permeance (40 LMH h-1)	677:713	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	3	10	theme	rheological	422:432	arg1	characterizations					465:481	the rheological, morphological, and structural characterizations	418:481	the rheological, morphological, and structural characterizations	418:481	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	6	11	theme	antifouling	1105:1115	arg1	ability					1117:1123	antifouling ability	1105:1123	antifouling ability	1105:1123	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	1	12	theme	suspension	186:195	arg1	behavior					197:204	suspension behavior	186:204	suspension behavior	186:204	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	6	13	theme	mechanical	1037:1046	arg1	strength					1048:1055	high mechanical strength	1032:1055	high mechanical strength	1032:1055	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	4	14	theme	superior	677:684	arg1	h-1					710:712	40 LMH h-1	703:712	40 LMH h-1	703:712	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	14	theme	superior	677:684	arg1	permeance					692:700	superior water permeance	677:700	superior water permeance (40 LMH h-1)	677:713	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	2	15	theme	ChNF	325:328	arg1	formation					312:320	the domain formation	301:320	the domain formation of ChNF derived by coordinative interaction	301:364	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	3	16	theme	morphological	435:447	arg1	characterizations					465:481	the rheological, morphological, and structural characterizations	418:481	the rheological, morphological, and structural characterizations	418:481	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	0	17	theme	Pore-size	0:8	arg1	control					10:16	Pore-size control	0:16	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.	0:89	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.					
34742448	1	18	theme	environmentally	99:113	arg1	membranes					146:154	environmentally benign chitin nanofiber (ChNF) membranes	99:154	environmentally benign chitin nanofiber (ChNF) membranes	99:154	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	2	19	theme	size-tunable	385:396	arg1	membranes					398:406	pore size-tunable membranes	380:406	pore size-tunable membranes	380:406	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	0	20	theme	chitin	21:26	arg1	membrane					50:57	chitin nanofibrous composite membrane	21:57	chitin nanofibrous composite membrane using metal-organic frameworks	21:88	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.					
34742448	6	21	theme	high	1032:1035	arg1	strength					1048:1055	high mechanical strength	1032:1055	high mechanical strength	1032:1055	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	3	22	theme	relative	596:603	arg1	concentration					605:617	the relative concentration	592:617	the relative concentration	592:617	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	1	23	theme	benign	115:120	arg1	ChNF					140:143	ChNF	140:143	ChNF	140:143	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	1	23	theme	benign	115:120	arg1	nanofiber					129:137	benign chitin nanofiber	115:137	environmentally benign chitin nanofiber (ChNF) membranes	99:154	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	4	24	theme	30-ChNF	651:657	arg1	membrane					659:666	the 30-ChNF membrane	647:666	the 30-ChNF membrane	647:666	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	25	theme	rejection	740:748	arg1	rate					750:753	a high rejection rate	733:753	a high rejection rate (>80% for all organic dyes)	733:781	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	6	26	theme	experimental	919:930	arg1	results					932:938	The experimental results	915:938	The experimental results	915:938	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	4	27	theme	organic	769:775	arg1	dyes					777:780	all organic dyes	765:780	all organic dyes	765:780	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	1	28	theme	chitin	122:127	arg1	ChNF					140:143	ChNF	140:143	ChNF	140:143	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	1	28	theme	chitin	122:127	arg1	nanofiber					129:137	benign chitin nanofiber	115:137	environmentally benign chitin nanofiber (ChNF) membranes	99:154	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	3	29	theme	driving	488:494	arg1	force					496:500	the driving force	484:500	the driving force of pore-size control	484:521	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	0	30	theme	composite	40:48	arg1	membrane					50:57	chitin nanofibrous composite membrane	21:57	chitin nanofibrous composite membrane using metal-organic frameworks	21:88	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.					
34742448	3	31	theme	control	515:521	arg1	force					496:500	the driving force	484:500	the driving force of pore-size control	484:521	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	6	32	with	MOF	1023:1025	arg1	ability					1117:1123	antifouling ability	1105:1123	antifouling ability	1105:1123	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	6	32	with	MOF	1023:1025	arg1	strength					1048:1055	high mechanical strength	1032:1055	high mechanical strength	1032:1055	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	6	32	with	MOF	1023:1025	arg1	durability					1063:1072	good durability	1058:1072	good durability	1058:1072	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	6	32	with	MOF	1023:1025	arg1	flux					1080:1083	high flux	1075:1083	high flux	1075:1083	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	6	32	with	MOF	1023:1025	arg1	rejection					1090:1098	dye rejection	1086:1098	dye rejection	1086:1098	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	6	33	theme	ChNF	1002:1005	arg1	membranes					1007:1015	pore tunable ChNF membranes	989:1015	pore tunable ChNF membranes	989:1015	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	0	34	theme	nanofibrous	28:38	arg1	membrane					50:57	chitin nanofibrous composite membrane	21:57	chitin nanofibrous composite membrane using metal-organic frameworks	21:88	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.					
34742448	5	35	theme	molecular	798:806	arg1	size					808:811	the molecular size	794:811	the molecular size cut-off of the composite membranes for dyes	794:855	Moreover, the molecular size cut-off of the composite membranes for dyes can be controlled in the range of less than 1 nm to 2 nm.					
34742448	5	36	theme	composite	828:836	arg1	membranes					838:846	the composite membranes	824:846	the composite membranes for dyes	824:855	Moreover, the molecular size cut-off of the composite membranes for dyes can be controlled in the range of less than 1 nm to 2 nm.					
34742448	6	37	theme	tunable	994:1000	arg1	membranes					1007:1015	pore tunable ChNF membranes	989:1015	pore tunable ChNF membranes	989:1015	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	2	38	theme	frameworks	246:255	arg1	introduction					211:222	The introduction	207:222	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes	207:292	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	6	39	theme	high	1075:1078	arg1	flux					1080:1083	high flux	1075:1083	high flux	1075:1083	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	1	40	theme	nanofiber	129:137	arg1	membranes					146:154	environmentally benign chitin nanofiber (ChNF) membranes	99:154	environmentally benign chitin nanofiber (ChNF) membranes	99:154	Herein, environmentally benign chitin nanofiber (ChNF) membranes were fabricated by regulating suspension behavior.					
34742448	0	41	theme	membrane	50:57	arg1	control					10:16	Pore-size control	0:16	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.	0:89	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.					
34742448	2	42	theme	zeolitic	227:234	arg1	ZIF-8					258:262	ZIF-8	258:262	ZIF-8	258:262	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	2	42	theme	zeolitic	227:234	arg1	frameworks					246:255	zeolitic imidazole frameworks	227:255	zeolitic imidazole frameworks (ZIF-8)	227:263	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	2	43	theme	imidazole	236:244	arg1	ZIF-8					258:262	ZIF-8	258:262	ZIF-8	258:262	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	2	43	theme	imidazole	236:244	arg1	frameworks					246:255	zeolitic imidazole frameworks	227:255	zeolitic imidazole frameworks (ZIF-8)	227:263	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	2	44	theme	coordinative	341:352	arg1	interaction					354:364	coordinative interaction	341:364	coordinative interaction	341:364	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	2	45	theme	domain	305:310	arg1	formation					312:320	the domain formation	301:320	the domain formation of ChNF derived by coordinative interaction	301:364	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	2	46	theme	composite	274:282	arg1	membranes					284:292	the composite membranes	270:292	the composite membranes	270:292	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	4	47	theme	high	735:738	arg1	rate					750:753	a high rejection rate	733:753	a high rejection rate (>80% for all organic dyes)	733:781	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	48	theme	critical	623:630	arg1	concentration					632:644	critical concentration	623:644	critical concentration	623:644	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	5	49	dep	2 nm	909:912	arg1	to					906:907	to	906:907	to	906:907	Moreover, the molecular size cut-off of the composite membranes for dyes can be controlled in the range of less than 1 nm to 2 nm.					
34742448	6	50	theme	good	1058:1061	arg1	durability					1063:1072	good durability	1058:1072	good durability	1058:1072	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	4	51	dep	rate	750:753	arg1	%					759:759	>80%	756:759	>80% for all organic dyes	756:780	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	52	attach	presents	668:675	arg1	concentration					632:644	critical concentration	623:644	critical concentration	623:644	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	4	52	attach	presents	668:675	arg2	membrane					659:666	the 30-ChNF membrane	647:666	the 30-ChNF membrane	647:666	At critical concentration, the 30-ChNF membrane presents superior water permeance (40 LMH h-1) while maintaining a high rejection rate (>80% for all organic dyes).					
34742448	2	53	theme	pore	380:383	arg1	membranes					398:406	pore size-tunable membranes	380:406	pore size-tunable membranes	380:406	The introduction of zeolitic imidazole frameworks (ZIF-8) into the composite membranes led to the domain formation of ChNF derived by coordinative interaction, resulting in pore size-tunable membranes.					
34742448	3	54	theme	structural	454:463	arg1	characterizations					465:481	the rheological, morphological, and structural characterizations	418:481	the rheological, morphological, and structural characterizations	418:481	Based on the rheological, morphological, and structural characterizations, the driving force of pore-size control was studied in the aqueous suspension of ChNF and ZIF-8 according to the relative concentration.					
34742448	0	55	theme	metal-organic	65:77	arg1	frameworks					79:88	metal-organic frameworks	65:88	metal-organic frameworks	65:88	Pore-size control of chitin nanofibrous composite membrane using metal-organic frameworks.					
34742448	6	56	theme	dye	1086:1088	arg1	rejection					1090:1098	dye rejection	1086:1098	dye rejection	1086:1098	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
34742448	6	57	theme	pore	989:992	arg1	membranes					1007:1015	pore tunable ChNF membranes	989:1015	pore tunable ChNF membranes	989:1015	The experimental results provide a simple strategy for the preparation of pore tunable ChNF membranes using MOF with high mechanical strength, good durability, high flux, dye rejection, and antifouling ability.					
32979440	6	0	theme	biphasic	1000:1007	arg1	behaviour					1009:1017	biphasic behaviour	1000:1017	biphasic behaviour	1000:1017	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	10	1	theme	successful	1559:1568	arg1	delivery					1577:1584	successful ocular delivery	1559:1584	successful ocular delivery	1559:1584	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	0	2	theme	ocular	94:99	arg1	delivery					101:108	improved ocular delivery	85:108	improved ocular delivery of dorzolamide	85:123	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	6	3	dep	12 h	1089:1092	arg1	up					1083:1084	up	1083:1084	up	1083:1084	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	7	4	theme	fold	1144:1147	arg1	enhancement					1149:1159	many fold enhancement	1139:1159	many fold enhancement	1139:1159	The corneal flux experiment showed many fold enhancement in permeation across goat cornea.					
32979440	4	5	theme	particle	612:619	arg1	size					621:624	particle size	612:624	particle size	612:624	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	0	6	theme	dorzolamide	113:123	arg1	delivery					101:108	improved ocular delivery	85:108	improved ocular delivery of dorzolamide	85:123	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	9	7	theme	assessment	1318:1327	arg1	study					1341:1345	the histopathological assessment and HET-CAM study	1296:1345	the histopathological assessment and HET-CAM study	1296:1345	Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration.					
32979440	10	8	theme	optimized	1498:1506	arg1	safe					1527:1530	safe	1527:1530	safe	1527:1530	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	10	8	theme	optimized	1498:1506	arg1	DRZ-CS-PCL-NPs					1508:1521	the optimized DRZ-CS-PCL-NPs	1494:1521	the optimized DRZ-CS-PCL-NPs	1494:1521	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	0	9	dep	In	126:127	arg1	vitro					129:133	vitro	129:133	vitro	129:133	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	9	10	dep	assessment	1318:1327	arg1	the					1296:1298	the	1296:1298	the	1296:1298	Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration.					
32979440	4	11	theme	polydispersity	627:640	arg1	index					642:646	polydispersity index	627:646	polydispersity index	627:646	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	10	12	contain	have	1536:1539	arg1	safe					1527:1530	safe	1527:1530	safe	1527:1530	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	10	12	contain	have	1536:1539	arg1	DRZ-CS-PCL-NPs					1508:1521	the optimized DRZ-CS-PCL-NPs	1494:1521	the optimized DRZ-CS-PCL-NPs	1494:1521	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	10	12	contain	have	1536:1539	arg2	potential					1545:1553	the potential	1541:1553	the potential for successful ocular delivery and improved therapeutic efficacy	1541:1618	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	7	13	theme	goat	1182:1185	arg1	cornea					1187:1192	goat cornea	1182:1192	goat cornea	1182:1192	The corneal flux experiment showed many fold enhancement in permeation across goat cornea.					
32979440	3	14	theme	polyvinyl	582:590	arg1	alcohol					592:598	polyvinyl alcohol	582:598	polyvinyl alcohol (1.5%)	582:605	The optimized DRZ-CS-PCL-NPs prepared with the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%).					
32979440	3	14	theme	polyvinyl	582:590	arg1	%					604:604	1.5%	601:604	1.5%	601:604	The optimized DRZ-CS-PCL-NPs prepared with the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%).					
32979440	1	15	theme	research	182:189	arg1	work					191:194	The present research work	170:194	The present research work	170:194	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	4	16	theme	DRZ-CS-PCL-NPs	706:719	arg1	efficiency					682:691	encapsulation efficiency	668:691	encapsulation efficiency	668:691	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	4	16	theme	DRZ-CS-PCL-NPs	706:719	arg1	size					621:624	particle size	612:624	particle size	612:624	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	4	16	theme	DRZ-CS-PCL-NPs	706:719	arg1	index					642:646	polydispersity index	627:646	polydispersity index	627:646	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	4	16	theme	DRZ-CS-PCL-NPs	706:719	arg1	potential					654:662	zeta potential	649:662	zeta potential	649:662	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	10	17	theme	therapeutic	1599:1609	arg1	efficacy					1611:1618	improved therapeutic efficacy	1590:1618	improved therapeutic efficacy	1590:1618	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	6	18	theme	DRZ	965:967	arg1	release					969:975	The DRZ release	961:975	The DRZ release from CS-PCL-NPs	961:991	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	9	19	theme	histopathological	1300:1316	arg1	assessment					1318:1327	histopathological assessment	1300:1327	histopathological assessment	1300:1327	Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration.					
32979440	3	20	theme	alcohol	592:598	arg1	composition					521:531	the composition	517:531	the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%)	517:605	The optimized DRZ-CS-PCL-NPs prepared with the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%).					
32979440	1	21	theme	present	174:180	arg1	work					191:194	The present research work	170:194	The present research work	170:194	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	5	22	theme	dependent	823:831	arg1	variables					858:866	The dependent and independent response variables	819:866	The dependent and independent response variables	819:866	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	4	23	theme	optimized	696:704	arg1	DRZ-CS-PCL-NPs					706:719	optimized DRZ-CS-PCL-NPs	696:719	optimized DRZ-CS-PCL-NPs	696:719	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	2	24	theme	three-level	442:452	arg1	design					466:471	the three-factor three-level Box-Behnken design	425:471	the three-factor three-level Box-Behnken design	425:471	The nanoparticles were prepared by single-step emulsification technique and optimized using the three-factor three-level Box-Behnken design.					
32979440	5	25	theme	excellent	875:883	arg1	correlation					885:895	excellent correlation	875:895	excellent correlation	875:895	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	1	26	theme	improved	307:314	arg1	delivery					323:330	improved ocular delivery	307:330	improved ocular delivery	307:330	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	10	27	theme	ocular	1570:1575	arg1	delivery					1577:1584	successful ocular delivery	1559:1584	successful ocular delivery	1559:1584	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	5	28	theme	optimized	935:943	arg1	DRZ-CS-PCL-NPs					945:958	the optimized DRZ-CS-PCL-NPs	931:958	the optimized DRZ-CS-PCL-NPs	931:958	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	2	29	theme	three-factor	429:440	arg1	design					466:471	the three-factor three-level Box-Behnken design	425:471	the three-factor three-level Box-Behnken design	425:471	The nanoparticles were prepared by single-step emulsification technique and optimized using the three-factor three-level Box-Behnken design.					
32979440	1	30	theme	ocular	316:321	arg1	delivery					323:330	improved ocular delivery	307:330	improved ocular delivery	307:330	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	9	31	theme	HET-CAM	1333:1339	arg1	study					1341:1345	the histopathological assessment and HET-CAM study	1296:1345	the histopathological assessment and HET-CAM study	1296:1345	Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration.					
32979440	4	32	theme	encapsulation	668:680	arg1	efficiency					682:691	encapsulation efficiency	668:691	encapsulation efficiency	668:691	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	8	33	theme	higher	1229:1234	arg1	strength					1249:1256	3.7 fold higher mucoadhesive strength	1220:1256	3.7 fold higher mucoadhesive strength	1220:1256	DRZ-CS-PCL-NPs exhibited 3.7 fold higher mucoadhesive strength compared to the control.					
32979440	8	34	theme	mucoadhesive	1236:1247	arg1	strength					1249:1256	3.7 fold higher mucoadhesive strength	1220:1256	3.7 fold higher mucoadhesive strength	1220:1256	DRZ-CS-PCL-NPs exhibited 3.7 fold higher mucoadhesive strength compared to the control.					
32979440	3	35	theme	optimized	478:486	arg1	DRZ-CS-PCL-NPs					488:501	The optimized DRZ-CS-PCL-NPs	474:501	The optimized DRZ-CS-PCL-NPs	474:501	The optimized DRZ-CS-PCL-NPs prepared with the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%).					
32979440	7	36	theme	many	1139:1142	arg1	enhancement					1149:1159	many fold enhancement	1139:1159	many fold enhancement	1139:1159	The corneal flux experiment showed many fold enhancement in permeation across goat cornea.					
32979440	0	37	theme	chitosan	31:38	arg1	development					16:26	development	16:26	development of chitosan	16:38	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	5	38	theme	DRZ-CS-PCL-NPs	945:958	arg1	rationality					916:926	the rationality	912:926	signifying the rationality of the optimized DRZ-CS-PCL-NPs	901:958	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	5	38	theme	DRZ-CS-PCL-NPs	945:958	arg1	correlation					885:895	excellent correlation	875:895	excellent correlation	875:895	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	4	39	dep	size	621:624	arg1	The					608:610	The	608:610	The	608:610	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
32979440	6	40	theme	study	1097:1101	arg1	12 h					1089:1092	12 h	1089:1092	12 h of study	1089:1101	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	3	41	theme	polycaprolactone	536:551	arg1	composition					521:531	the composition	517:531	the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%)	517:605	The optimized DRZ-CS-PCL-NPs prepared with the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%).					
32979440	5	42	theme	response	849:856	arg1	variables					858:866	The dependent and independent response variables	819:866	The dependent and independent response variables	819:866	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	6	43	theme	burst	1032:1036	arg1	release					1038:1044	initial burst release	1024:1044	initial burst release for 2 h after that sustained-release up to 12 h of study	1024:1101	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	0	44	theme	toxicity	148:155	arg1	assessments					157:167	toxicity assessments	148:167	toxicity assessments	148:167	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	3	45	theme	chitosan	562:569	arg1	composition					521:531	the composition	517:531	the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%)	517:605	The optimized DRZ-CS-PCL-NPs prepared with the composition of polycaprolactone (60 mg), chitosan (0.6%) and polyvinyl alcohol (1.5%).					
32979440	0	46	theme	polycaprolactone	50:65	arg1	nanoparticles					67:79	polycaprolactone nanoparticles	50:79	polycaprolactone nanoparticles for improved ocular delivery of dorzolamide	50:123	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	6	47	theme	initial	1024:1030	arg1	release					1038:1044	initial burst release	1024:1044	initial burst release for 2 h after that sustained-release up to 12 h of study	1024:1101	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	10	48	theme	present	1454:1460	arg1	study					1462:1466	the present study	1450:1466	the present study	1450:1466	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	2	49	theme	emulsification	380:393	arg1	technique					395:403	single-step emulsification technique	368:403	single-step emulsification technique	368:403	The nanoparticles were prepared by single-step emulsification technique and optimized using the three-factor three-level Box-Behnken design.					
32979440	7	50	theme	corneal	1108:1114	arg1	experiment					1121:1130	The corneal flux experiment	1104:1130	The corneal flux experiment	1104:1130	The corneal flux experiment showed many fold enhancement in permeation across goat cornea.					
32979440	2	51	theme	single-step	368:378	arg1	technique					395:403	single-step emulsification technique	368:403	single-step emulsification technique	368:403	The nanoparticles were prepared by single-step emulsification technique and optimized using the three-factor three-level Box-Behnken design.					
32979440	1	52	theme	dorzolamide-loaded	220:237	arg1	DRZ-CS-PCL-NPs					287:300	DRZ-CS-PCL-NPs	287:300	DRZ-CS-PCL-NPs	287:300	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	1	52	theme	dorzolamide-loaded	220:237	arg1	nanoparticles					272:284	dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles	220:284	dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery	220:330	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	7	53	theme	flux	1116:1119	arg1	experiment					1121:1130	The corneal flux experiment	1104:1130	The corneal flux experiment	1104:1130	The corneal flux experiment showed many fold enhancement in permeation across goat cornea.					
32979440	10	54	theme	improved	1590:1597	arg1	efficacy					1611:1618	improved therapeutic efficacy	1590:1618	improved therapeutic efficacy	1590:1618	Therefore, from the present study, it can be concluded that the optimized DRZ-CS-PCL-NPs are safe and have the potential for successful ocular delivery and improved therapeutic efficacy.					
32979440	1	55	theme	chitosan-coated	239:253	arg1	DRZ-CS-PCL-NPs					287:300	DRZ-CS-PCL-NPs	287:300	DRZ-CS-PCL-NPs	287:300	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	1	55	theme	chitosan-coated	239:253	arg1	nanoparticles					272:284	dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles	220:284	dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery	220:330	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	0	56	theme	improved	85:92	arg1	delivery					101:108	improved ocular delivery	85:108	improved ocular delivery of dorzolamide	85:123	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	5	57	theme	signifying	901:910	arg1	rationality					916:926	the rationality	912:926	signifying the rationality of the optimized DRZ-CS-PCL-NPs	901:958	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	2	58	theme	Box-Behnken	454:464	arg1	design					466:471	the three-factor three-level Box-Behnken design	425:471	the three-factor three-level Box-Behnken design	425:471	The nanoparticles were prepared by single-step emulsification technique and optimized using the three-factor three-level Box-Behnken design.					
32979440	1	59	theme	polycaprolactone	255:270	arg1	DRZ-CS-PCL-NPs					287:300	DRZ-CS-PCL-NPs	287:300	DRZ-CS-PCL-NPs	287:300	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	1	59	theme	polycaprolactone	255:270	arg1	nanoparticles					272:284	dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles	220:284	dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery	220:330	The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery.					
32979440	6	60	from	CS-PCL-NPs	982:991	arg1	release					969:975	The DRZ release	961:975	The DRZ release from CS-PCL-NPs	961:991	The DRZ release from CS-PCL-NPs showed biphasic behaviour with initial burst release for 2 h after that sustained-release up to 12 h of study.					
32979440	9	61	theme	ocular	1411:1416	arg1	administration					1418:1431	ocular administration	1411:1431	ocular administration	1411:1431	Furthermore, the histopathological assessment and HET-CAM study revealed that the DRZ-CS-PCL-NPs were non-irritant and safe for ocular administration.					
32979440	5	62	theme	independent	837:847	arg1	variables					858:866	The dependent and independent response variables	819:866	The dependent and independent response variables	819:866	The dependent and independent response variables showed excellent correlation and signifying the rationality of the optimized DRZ-CS-PCL-NPs.					
32979440	0	63	dep	ex	136:137	arg1	vivo					139:142	vivo	139:142	vivo	139:142	Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.					
32979440	4	64	theme	zeta	649:652	arg1	potential					654:662	zeta potential	649:662	zeta potential	649:662	The particle size, polydispersity index, zeta potential and encapsulation efficiency of optimized DRZ-CS-PCL-NPs were found to be 192.38 ± 6.42 nm, 0.18 ± 0.04, +5.21 ± 1.24 mV, and 72.48 ± 5.62%, respectively.					
33172991	1	0	theme	Carbohydrate	90:101	arg1	polymers					103:110	Carbohydrate polymers	90:110	Carbohydrate polymers	90:110	Carbohydrate polymers exhibit incredible chemical and structural diversity, yet are produced by polymerases without a template to guide length and composition.					
33172991	4	1	theme	major	878:882	arg1	defects					884:890	major defects	878:890	major defects in cell growth and division	878:918	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	9	2	theme	cell	1723:1726	arg1	enzyme					1737:1742	this key cell envelope enzyme	1714:1742	this key cell envelope enzyme	1714:1742	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
33172991	6	3	theme	lipid	1151:1155	arg1	units					1165:1169	lipid starter units	1151:1169	lipid starter units	1151:1169	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	0	4	theme	starter	75:81	arg1	units					83:87	free starter units	70:87	free starter units	70:87	Lipoteichoic acid polymer length is determined by competition between free starter units.					
33172991	8	5	theme	growing	1493:1499	arg1	polymer					1501:1507	the growing polymer	1489:1507	the growing polymer	1489:1507	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	8	6	theme	lipid	1473:1477	arg1	anchor					1479:1484	the lipid anchor	1469:1484	the lipid anchor of the growing polymer	1469:1507	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	6	7	theme	LtaS	1082:1085	arg1	length					1047:1052	polymer length	1039:1052	polymer length	1039:1052	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	6	7	theme	LtaS	1082:1085	arg1	property					1070:1077	an intrinsic property	1057:1077	an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units	1057:1169	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	5	8	theme	LTA	938:940	arg1	length					950:955	LTA polymer length	938:955	LTA polymer length	938:955	To determine how LTA polymer length is controlled, we reconstituted Staphylococcus aureus LtaS in vitro.					
33172991	8	9	theme	surprising	1392:1401	arg1	result					1403:1408	this surprising result	1387:1408	this surprising result	1387:1408	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	6	10	theme	starter	1157:1163	arg1	units					1165:1169	lipid starter units	1151:1169	lipid starter units	1151:1169	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	4	11	theme	PG-anchored	768:778	arg1	polymers					784:791	PG-anchored LTA polymers	768:791	PG-anchored LTA polymers	768:791	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	3	12	from	leaflet	663:669	arg1	embedded					633:640	embedded	633:640	embedded	633:640	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	8	13	from	site	1526:1529	arg1	enzyme					1538:1543	the enzyme	1534:1543	the enzyme	1534:1543	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	7	14	theme	preferred	1264:1272	arg1	unit					1290:1293	the preferred Glc2DAG starter unit	1260:1293	the preferred Glc2DAG starter unit	1260:1293	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	7	15	theme	Glc2DAG	1274:1280	arg1	unit					1290:1293	the preferred Glc2DAG starter unit	1260:1293	the preferred Glc2DAG starter unit	1260:1293	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	5	16	theme	polymer	942:948	arg1	length					950:955	LTA polymer length	938:955	LTA polymer length	938:955	To determine how LTA polymer length is controlled, we reconstituted Staphylococcus aureus LtaS in vitro.					
33172991	2	17	theme	polymers	280:287	arg1	critical					292:299	critical	292:299	critical	292:299	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	2	17	theme	polymers	280:287	arg1	length					257:262	the length	253:262	the length of carbohydrate polymers	253:287	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	3	18	from	embedded	633:640	arg1	leaflet					663:669	the extracellular leaflet	645:669	the extracellular leaflet of the cell membrane	645:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	7	19	theme	Glc2DAG	1311:1317	arg1	use					1304:1306	the use	1300:1306	the use of Glc2DAG	1300:1317	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	8	20	theme	binding	1518:1524	arg1	site					1526:1529	its binding site	1514:1529	its binding site on the enzyme	1514:1543	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	2	21	theme	carbohydrate	267:278	arg1	polymers					280:287	carbohydrate polymers	267:287	carbohydrate polymers	267:287	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	2	22	theme	important	398:406	arg1	area					408:411	an important area	395:411	an important area of investigation	395:428	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	0	23	theme	acid	13:16	arg1	length					26:31	Lipoteichoic acid polymer length	0:31	Lipoteichoic acid polymer length	0:31	Lipoteichoic acid polymer length is determined by competition between free starter units.					
33172991	3	24	theme	lipoteichoic	545:556	arg1	LtaS					573:576	LtaS	573:576	LtaS	573:576	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	3	24	theme	lipoteichoic	545:556	arg1	synthase					563:570	lipoteichoic acid synthase	545:570	lipoteichoic acid synthase (LtaS)	545:577	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	7	25	theme	overall	1210:1216	arg1	faster					1249:1254	faster	1249:1254	faster	1249:1254	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	7	25	theme	overall	1210:1216	arg1	rate					1227:1230	the overall reaction rate	1206:1230	the overall reaction rate	1206:1230	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	7	26	theme	reaction	1218:1225	arg1	faster					1249:1254	faster	1249:1254	faster	1249:1254	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	7	26	theme	reaction	1218:1225	arg1	rate					1227:1230	the overall reaction rate	1206:1230	the overall reaction rate	1206:1230	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	4	27	theme	starter	750:756	arg1	unit					758:761	an alternative starter unit	735:761	an alternative starter unit	735:761	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	4	27	theme	starter	750:756	arg1	phosphatidylglycerol					706:725	phosphatidylglycerol	706:725	phosphatidylglycerol (PG)	706:730	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	8	28	theme	polymer	1501:1507	arg1	anchor					1479:1484	the lipid anchor	1469:1484	the lipid anchor of the growing polymer	1469:1507	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	3	29	theme	Gram-positive	436:448	arg1	bacteria					450:457	Most Gram-positive bacteria	431:457	Most Gram-positive bacteria	431:457	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	0	30	theme	Lipoteichoic	0:11	arg1	length					26:31	Lipoteichoic acid polymer length	0:31	Lipoteichoic acid polymer length	0:31	Lipoteichoic acid polymer length is determined by competition between free starter units.					
33172991	4	31	theme	long	856:859	arg1	polymers					861:868	these abnormally long polymers	839:868	these abnormally long polymers	839:868	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	2	32	theme	biological	311:320	arg1	functions					322:330	their biological functions	305:330	their biological functions	305:330	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	8	33	theme	starter	1416:1422	arg1	units					1424:1428	free starter units	1411:1428	free starter units	1411:1428	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	4	34	theme	alternative	738:748	arg1	unit					758:761	an alternative starter unit	735:761	an alternative starter unit	735:761	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	4	34	theme	alternative	738:748	arg1	phosphatidylglycerol					706:725	phosphatidylglycerol	706:725	phosphatidylglycerol (PG)	706:730	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	7	35	theme	shorter	1328:1334	arg1	polymers					1336:1343	shorter polymers	1328:1343	shorter polymers	1328:1343	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	8	36	theme	simple	1359:1364	arg1	mechanism					1366:1374	a simple mechanism	1357:1374	a simple mechanism to explain this surprising result	1357:1408	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	3	37	theme	lipoteichoic	496:507	arg1	LTA					516:518	LTA	516:518	LTA	516:518	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	3	37	theme	lipoteichoic	496:507	arg1	acids					509:513	lipoteichoic acids	496:513	lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane	496:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	3	38	theme	diglucosyl-diacylglycerol	584:608	arg1	unit					628:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit	582:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane	582:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	4	39	from	defects	884:890	arg1	cell					895:898	cell growth and division	895:918	cell growth and division	895:918	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	3	40	theme	acid	558:561	arg1	LtaS					573:576	LtaS	573:576	LtaS	573:576	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	3	40	theme	acid	558:561	arg1	synthase					563:570	lipoteichoic acid synthase	545:570	lipoteichoic acid synthase (LtaS)	545:577	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	8	41	theme	free	1411:1414	arg1	units					1424:1428	free starter units	1411:1428	free starter units	1411:1428	We propose a simple mechanism to explain this surprising result: free starter units terminate polymerization by displacing the lipid anchor of the growing polymer from its binding site on the enzyme.					
33172991	7	42	theme	starter	1282:1288	arg1	unit					1290:1293	the preferred Glc2DAG starter unit	1260:1293	the preferred Glc2DAG starter unit	1260:1293	Polymerization is processive, and the overall reaction rate is substantially faster for the preferred Glc2DAG starter unit, yet the use of Glc2DAG leads to shorter polymers.					
33172991	6	43	theme	polymer	1039:1045	arg1	length					1047:1052	polymer length	1039:1052	polymer length	1039:1052	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	6	43	theme	polymer	1039:1045	arg1	property					1070:1077	an intrinsic property	1057:1077	an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units	1057:1169	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	2	44	theme	investigation	416:428	arg1	area					408:411	an important area	395:411	an important area of investigation	395:428	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	3	45	theme	Most	431:434	arg1	bacteria					450:457	Most Gram-positive bacteria	431:457	Most Gram-positive bacteria	431:457	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	3	46	theme	extracellular	649:661	arg1	leaflet					663:669	the extracellular leaflet	645:669	the extracellular leaflet of the cell membrane	645:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	3	47	theme	starter	620:626	arg1	unit					628:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit	582:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane	582:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	6	48	theme	intrinsic	1060:1068	arg1	length					1047:1052	polymer length	1039:1052	polymer length	1039:1052	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	6	48	theme	intrinsic	1060:1068	arg1	property					1070:1077	an intrinsic property	1057:1077	an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units	1057:1169	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	9	49	theme	most	1579:1582	arg1	bacteria					1598:1605	most Gram-positive bacteria	1579:1605	most Gram-positive bacteria	1579:1605	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
33172991	9	50	theme	key	1719:1721	arg1	enzyme					1737:1742	this key cell envelope enzyme	1714:1742	this key cell envelope enzyme	1714:1742	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
33172991	1	51	theme	incredible	120:129	arg1	diversity					155:163	incredible chemical and structural diversity	120:163	incredible chemical and structural diversity	120:163	Carbohydrate polymers exhibit incredible chemical and structural diversity, yet are produced by polymerases without a template to guide length and composition.					
33172991	4	52	dep	cell	895:898	arg1	division					911:918	division	911:918	division	911:918	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	4	52	dep	cell	895:898	arg1	growth					900:905	growth	900:905	growth	900:905	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	3	53	theme	Glc2DAG	611:617	arg1	unit					628:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit	582:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane	582:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	1	54	theme	chemical	131:138	arg1	diversity					155:163	incredible chemical and structural diversity	120:163	incredible chemical and structural diversity	120:163	Carbohydrate polymers exhibit incredible chemical and structural diversity, yet are produced by polymerases without a template to guide length and composition.					
33172991	3	55	theme	embedded	633:640	arg1	unit					628:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit	582:631	a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane	582:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	9	56	theme	envelope	1728:1735	arg1	enzyme					1737:1742	this key cell envelope enzyme	1714:1742	this key cell envelope enzyme	1714:1742	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
33172991	6	57	theme	units	1165:1169	arg1	concentration					1134:1146	concentration	1134:1146	concentration	1134:1146	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	6	57	theme	units	1165:1169	arg1	identity					1121:1128	identity	1121:1128	identity	1121:1128	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	4	58	theme	LTA	780:782	arg1	polymers					784:791	PG-anchored LTA polymers	768:791	PG-anchored LTA polymers	768:791	LtaS can use phosphatidylglycerol (PG) as an alternative starter unit, but PG-anchored LTA polymers are significantly longer, and cells that make these abnormally long polymers exhibit major defects in cell growth and division.					
33172991	3	59	theme	cell	678:681	arg1	membrane					683:690	the cell membrane	674:690	the cell membrane	674:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	5	60	theme	aureus	1004:1009	arg1	LtaS					1011:1014	Staphylococcus aureus LtaS	989:1014	Staphylococcus aureus LtaS	989:1014	To determine how LTA polymer length is controlled, we reconstituted Staphylococcus aureus LtaS in vitro.					
33172991	1	61	theme	structural	144:153	arg1	diversity					155:163	incredible chemical and structural diversity	120:163	incredible chemical and structural diversity	120:163	Carbohydrate polymers exhibit incredible chemical and structural diversity, yet are produced by polymerases without a template to guide length and composition.					
33172991	0	62	theme	free	70:73	arg1	units					83:87	free starter units	70:87	free starter units	70:87	Lipoteichoic acid polymer length is determined by competition between free starter units.					
33172991	3	63	theme	membrane	683:690	arg1	leaflet					663:669	the extracellular leaflet	645:669	the extracellular leaflet of the cell membrane	645:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	6	64	dep	identity	1121:1128	arg1	the					1117:1119	the	1117:1119	the	1117:1119	We show that polymer length is an intrinsic property of LtaS that is directly regulated by the identity and concentration of lipid starter units.					
33172991	3	65	theme	anionic	467:473	arg1	glycopolymers					475:487	anionic glycopolymers	467:487	anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane	467:690	Most Gram-positive bacteria produce anionic glycopolymers called lipoteichoic acids (LTA) that are synthesized by lipoteichoic acid synthase (LtaS) on a diglucosyl-diacylglycerol (Glc2DAG) starter unit embedded in the extracellular leaflet of the cell membrane.					
33172991	9	66	theme	enzyme	1737:1742	arg1	inhibitors					1700:1709	inhibitors	1700:1709	inhibitors of this key cell envelope enzyme	1700:1742	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
33172991	9	67	theme	reconstituted	1643:1655	arg1	system					1657:1662	this reconstituted system	1638:1662	this reconstituted system	1638:1662	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
33172991	0	68	theme	polymer	18:24	arg1	length					26:31	Lipoteichoic acid polymer length	0:31	Lipoteichoic acid polymer length	0:31	Lipoteichoic acid polymer length is determined by competition between free starter units.					
33172991	2	69	theme	polymer	377:383	arg1	length					385:390	polymer length	377:390	polymer length	377:390	As the length of carbohydrate polymers is critical for their biological functions, understanding the mechanisms that determine polymer length is an important area of investigation.					
33172991	9	70	theme	Gram-positive	1584:1596	arg1	bacteria					1598:1605	most Gram-positive bacteria	1579:1605	most Gram-positive bacteria	1579:1605	Because LtaS is conserved across most Gram-positive bacteria and is important for survival, this reconstituted system should be useful for characterizing inhibitors of this key cell envelope enzyme.					
32403297	9	0	dep	L.	1189:1190	arg1	reuteri					1192:1198	reuteri	1192:1198	reuteri	1192:1198	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	7	1	theme	probiotic	1027:1035	arg1	strains					1047:1053	nine different probiotic bacterial strains	1012:1053	nine different probiotic bacterial strains	1012:1053	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	10	2	theme	growth	1326:1331	arg1	experiments					1344:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	10	3	theme	in-vitro	1308:1315	arg1	experiments					1344:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	7	4	theme	strain	799:804	arg1	probiotics					806:815	bacterial mono strain probiotics	784:815	bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938	784:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	1	5	theme	probiotic	186:194	arg1	bacteria					196:203	probiotic bacteria	186:203	probiotic bacteria	186:203	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	10	6	theme	clinical	1518:1525	arg1	evaluation					1527:1536	further clinical evaluation	1510:1536	further clinical evaluation	1510:1536	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	2	7	theme	bacteria	288:295	arg1	growth					267:272	the growth	263:272	the growth of pathogenic bacteria in the gut	263:306	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	7	8	theme	bacterial	784:792	arg1	probiotics					806:815	bacterial mono strain probiotics	784:815	bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938	784:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	9	theme	multi	978:982	arg1	synbiotic					991:999	a multi strain synbiotic	976:999	a multi strain synbiotic	976:999	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	10	10	theme	further	1510:1516	arg1	evaluation					1527:1536	further clinical evaluation	1510:1536	further clinical evaluation	1510:1536	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	7	11	contain	containing	917:926	arg2	Pen					966:968	E/N, Oxy, Pen	956:968	Pen	966:968	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	11	contain	containing	917:926	arg1	strain					900:905	a multi strain probiotic	892:915	a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen)	892:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	11	contain	containing	917:926	arg2	strains					947:953	three L. rhamnosus strains	928:953	three L. rhamnosus strains (E/N, Oxy, Pen)	928:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	1	12	located	observed	205:212	arg2	effects					175:181	the beneficial effects	160:181	the beneficial effects of probiotic bacteria observed in humans	160:222	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	1	12	located	observed	205:212	arg1	humans					217:222	humans	217:222	humans	217:222	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	7	13	theme	E/N	956:958	arg1	strains					947:953	three L. rhamnosus strains	928:953	three L. rhamnosus strains (E/N, Oxy, Pen)	928:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	13	theme	E/N	956:958	arg1	Pen					966:968	E/N, Oxy, Pen	956:968	Pen	966:968	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	2	14	from	growth	267:272	arg1	gut					304:306	the gut	300:306	the gut	300:306	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	9	15	theme	L.	1189:1190	arg1	17938					1204:1208	L. reuteri DSM 17938	1189:1208	L. reuteri DSM 17938	1189:1208	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	10	16	contain	containing	1420:1429	arg1	categories					1409:1418	different categories	1399:1418	different categories containing probiotic microorganisms	1399:1454	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	10	16	contain	containing	1420:1429	arg2	microorganisms					1441:1454	probiotic microorganisms	1431:1454	probiotic microorganisms	1431:1454	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	3	17	theme	present	313:319	arg1	study					321:325	The present study	309:325	The present study	309:325	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	9	18	theme	DSM	1200:1202	arg1	17938					1204:1208	L. reuteri DSM 17938	1189:1208	L. reuteri DSM 17938	1189:1208	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	7	19	theme	L.	934:935	arg1	strains					947:953	three L. rhamnosus strains	928:953	three L. rhamnosus strains (E/N, Oxy, Pen)	928:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	19	theme	L.	934:935	arg1	Pen					966:968	E/N, Oxy, Pen	956:968	Pen	966:968	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	10	20	from	categories	1409:1418	arg1	products					1385:1392	products	1385:1392	products from different categories containing probiotic microorganisms	1385:1454	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	5	21	theme	Salmonella	577:586	arg1	difficile					657:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile were determined.					
32403297	2	22	from	gut	304:306	arg1	inhibition					249:258	a direct inhibition	240:258	a direct inhibition of the growth of pathogenic bacteria in the gut	240:306	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	6	23	contain	containing	741:750	arg2	Sac					767:769	Sac.	767:770	Sac.	767:770	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	23	contain	containing	741:750	arg1	strain					724:729	a yeast mono strain probiotic	711:739	a yeast mono strain probiotic containing Saccharomyces (Sac.)	711:771	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	23	contain	containing	741:750	arg1	products					697:704	Investigated products	684:704	Investigated products	684:704	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	23	contain	containing	741:750	arg2	Saccharomyces					752:764	Saccharomyces	752:764	Saccharomyces (Sac.)	752:771	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	4	24	theme	Escherichia	526:536	arg1	EPEC					548:551	Escherichia (E.) coli EPEC	526:551	Escherichia (E.) coli EPEC	526:551	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	0	25	theme	Product	81:87	arg1	Matters					101:107	Product Composition Matters	81:107	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.	0:108	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	9	26	theme	L.	1218:1219	arg1	strong					1257:1262	strong	1257:1262	strong	1257:1262	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	26	theme	L.	1218:1219	arg1	Oxy					1236:1238	Oxy	1236:1238	Oxy	1236:1238	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	26	theme	L.	1218:1219	arg1	mixture					1245:1251	Pen mixture	1241:1251	Pen mixture	1241:1251	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	26	theme	L.	1218:1219	arg1	E/N					1231:1233	the L. rhamnosus E/N	1214:1233	the L. rhamnosus E/N	1214:1233	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	26	theme	L.	1218:1219	arg1	boulardii					1148:1156	boulardii	1148:1156	boulardii	1148:1156	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	1	27	theme	activities	123:132	arg1	activities					123:132	activities	123:132	activities	123:132	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	1	27	theme	activities	123:132	arg1	variety					112:118	A variety	110:118	A variety of activities	110:132	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	3	28	theme	different	449:457	arg1	types					459:463	different types	449:463	different types of probiotics and a synbiotic	449:493	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	4	29	dep	Shigella	554:561	arg1	Sh					564:565	Sh	564:565	Sh.	564:566	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	4	30	theme	In-vitro	496:503	arg1	Shigella					554:561	Shigella	554:561	Shigella (Sh.)	554:567	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	4	30	theme	In-vitro	496:503	arg1	inhibition					512:521	In-vitro growth inhibition	496:521	In-vitro growth inhibition of Escherichia (E.) coli EPEC	496:551	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	10	31	used	used	1363:1366	arg2	experiments					1344:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	3	32	theme	strains	438:444	arg1	inhibition					383:392	head-to-head the in-vitro pathogen growth inhibition	341:392	head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains	341:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	0	33	theme	In-Vitro	0:7	arg1	Inhibition					16:25	In-Vitro Growth Inhibition	0:25	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.	0:108	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	5	34	theme	sonnei	569:574	arg1	difficile					657:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile were determined.					
32403297	3	35	theme	pathogen	367:374	arg1	growth					376:381	the in-vitro pathogen growth	354:381	head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains	341:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	9	36	theme	Pen	1241:1243	arg1	mixture					1245:1251	Pen mixture	1241:1251	Pen mixture	1241:1251	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	36	theme	Pen	1241:1243	arg1	E/N					1231:1233	the L. rhamnosus E/N	1214:1233	the L. rhamnosus E/N	1214:1233	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	3	37	theme	infectious	417:426	arg1	strains					438:444	clinically relevant infectious bacterial strains	397:444	clinically relevant infectious bacterial strains	397:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	10	38	theme	different	1399:1407	arg1	categories					1409:1418	different categories	1399:1418	different categories containing probiotic microorganisms	1399:1454	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	0	39	theme	Bacterial	30:38	arg1	Pathogens					40:48	Bacterial Pathogens	30:48	Bacterial Pathogens	30:48	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	4	40	theme	coli	543:546	arg1	EPEC					548:551	Escherichia (E.) coli EPEC	526:551	Escherichia (E.) coli EPEC	526:551	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	6	41	theme	yeast	713:717	arg1	strain					724:729	a yeast mono strain probiotic	711:739	a yeast mono strain probiotic containing Saccharomyces (Sac.)	711:771	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	41	theme	yeast	713:717	arg1	products					697:704	Investigated products	684:704	Investigated products	684:704	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	3	42	theme	synbiotic	485:493	arg1	types					459:463	different types	449:463	different types of probiotics and a synbiotic	449:493	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	7	43	theme	DSM	881:883	arg1	17938					885:889	L. reuteri DSM 17938	870:889	L. reuteri DSM 17938	870:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	9	44	theme	multi	1271:1275	arg1	synbiotic					1284:1292	the multi strain synbiotic	1267:1292	the multi strain synbiotic	1267:1292	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	0	45	dep	Inhibition	16:25	arg1	Matters					101:107	Product Composition Matters	81:107	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.	0:108	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	7	46	theme	L.	870:871	arg1	17938					885:889	L. reuteri DSM 17938	870:889	L. reuteri DSM 17938	870:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	5	47	theme	K.	618:619	arg1	difficile					657:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile were determined.					
32403297	6	48	theme	Investigated	684:695	arg1	strain					724:729	a yeast mono strain probiotic	711:739	a yeast mono strain probiotic containing Saccharomyces (Sac.)	711:771	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	48	theme	Investigated	684:695	arg1	products					697:704	Investigated products	684:704	Investigated products	684:704	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	7	49	dep	Pen	966:968	arg1	Oxy					961:963	E/N, Oxy, Pen	956:968	Oxy	961:963	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	50	theme	bacterial	1037:1045	arg1	strains					1047:1053	nine different probiotic bacterial strains	1012:1053	nine different probiotic bacterial strains	1012:1053	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	51	dep	L.	870:871	arg1	reuteri					873:879	reuteri	873:879	reuteri	873:879	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	2	52	theme	pathogenic	277:286	arg1	bacteria					288:295	pathogenic bacteria	277:295	pathogenic bacteria	277:295	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	10	53	theme	products	1497:1504	arg1	process					1486:1492	the selection process	1472:1492	the selection process of products for further clinical evaluation	1472:1536	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	7	54	theme	different	1017:1025	arg1	strains					1047:1053	nine different probiotic bacterial strains	1012:1053	nine different probiotic bacterial strains	1012:1053	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	2	55	theme	growth	267:272	arg1	inhibition					249:258	a direct inhibition	240:258	a direct inhibition of the growth of pathogenic bacteria in the gut	240:306	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	1	56	theme	beneficial	164:173	arg1	effects					175:181	the beneficial effects	160:181	the beneficial effects of probiotic bacteria observed in humans	160:222	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	10	57	theme	pathogen	1317:1324	arg1	experiments					1344:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	8	58	theme	pathogens	1117:1125	arg1	Inhibition					1103:1112	Inhibition	1103:1112	Inhibition of pathogens	1103:1125	Inhibition of pathogens was moderate by Sac.					
32403297	7	59	theme	strain	984:989	arg1	synbiotic					991:999	a multi strain synbiotic	976:999	a multi strain synbiotic	976:999	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	10	60	theme	Head-to-head	1295:1306	arg1	experiments					1344:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	1	61	theme	bacteria	196:203	arg1	effects					175:181	the beneficial effects	160:181	the beneficial effects of probiotic bacteria observed in humans	160:222	A variety of activities potentially contribute to the beneficial effects of probiotic bacteria observed in humans.					
32403297	7	62	theme	mono	794:797	arg1	probiotics					806:815	bacterial mono strain probiotics	784:815	bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938	784:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	5	63	theme	C.	653:654	arg1	difficile					657:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile	569:665	sonnei, Salmonella (S.) typhimurium, Klebsiella (K.) pneumoniae and Clostridioides (C.) difficile were determined.					
32403297	2	64	theme	direct	242:247	arg1	inhibition					249:258	a direct inhibition	240:258	a direct inhibition of the growth of pathogenic bacteria in the gut	240:306	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	7	65	theme	rhamnosus	937:945	arg1	strains					947:953	three L. rhamnosus strains	928:953	three L. rhamnosus strains (E/N, Oxy, Pen)	928:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	65	theme	rhamnosus	937:945	arg1	Pen					966:968	E/N, Oxy, Pen	956:968	Pen	966:968	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	0	66	theme	Composition	89:99	arg1	Matters					101:107	Product Composition Matters	81:107	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.	0:108	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	3	67	theme	in-vitro	358:365	arg1	growth					376:381	the in-vitro pathogen growth	354:381	head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains	341:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	10	68	theme	inhibition	1333:1342	arg1	experiments					1344:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments	1295:1354	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	9	69	theme	rhamnosus	1221:1229	arg1	strong					1257:1262	strong	1257:1262	strong	1257:1262	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	69	theme	rhamnosus	1221:1229	arg1	Oxy					1236:1238	Oxy	1236:1238	Oxy	1236:1238	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	69	theme	rhamnosus	1221:1229	arg1	mixture					1245:1251	Pen mixture	1241:1251	Pen mixture	1241:1251	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	69	theme	rhamnosus	1221:1229	arg1	E/N					1231:1233	the L. rhamnosus E/N	1214:1233	the L. rhamnosus E/N	1214:1233	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	69	theme	rhamnosus	1221:1229	arg1	boulardii					1148:1156	boulardii	1148:1156	boulardii	1148:1156	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	7	70	theme	probiotic	907:915	arg1	strain					900:905	a multi strain probiotic	892:915	a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen)	892:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	3	71	theme	head-to-head	341:352	arg1	inhibition					383:392	head-to-head the in-vitro pathogen growth inhibition	341:392	head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains	341:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	4	72	theme	growth	505:510	arg1	Shigella					554:561	Shigella	554:561	Shigella (Sh.)	554:567	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	4	72	theme	growth	505:510	arg1	inhibition					512:521	In-vitro growth inhibition	496:521	In-vitro growth inhibition of Escherichia (E.) coli EPEC	496:551	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	0	73	theme	Growth	9:14	arg1	Inhibition					16:25	In-Vitro Growth Inhibition	0:25	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.	0:108	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	7	74	theme	multi	894:898	arg1	strain					900:905	a multi strain probiotic	892:915	a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen)	892:969	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	3	75	theme	growth	376:381	arg1	inhibition					383:392	head-to-head the in-vitro pathogen growth inhibition	341:392	head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains	341:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	0	76	theme	Pathogens	40:48	arg1	Inhibition					16:25	In-Vitro Growth Inhibition	0:25	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.	0:108	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	0	77	dep	Probiotics	53:62	arg1	Synbiotic					70:78	Synbiotic	70:78	Synbiotic	70:78	In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters.					
32403297	2	78	from	inhibition	249:258	arg1	gut					304:306	the gut	300:306	the gut	300:306	Among these is a direct inhibition of the growth of pathogenic bacteria in the gut.					
32403297	3	79	theme	bacterial	428:436	arg1	strains					438:444	clinically relevant infectious bacterial strains	397:444	clinically relevant infectious bacterial strains	397:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	6	80	theme	probiotic	731:739	arg1	strain					724:729	a yeast mono strain probiotic	711:739	a yeast mono strain probiotic containing Saccharomyces (Sac.)	711:771	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	80	theme	probiotic	731:739	arg1	products					697:704	Investigated products	684:704	Investigated products	684:704	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	3	81	theme	probiotics	468:477	arg1	types					459:463	different types	449:463	different types of probiotics and a synbiotic	449:493	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	7	82	theme	prebiotic	1063:1071	arg1	FOS					1097:1099	FOS	1097:1099	FOS	1097:1099	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	82	theme	prebiotic	1063:1071	arg1	fructooligosaccharides					1073:1094	the prebiotic fructooligosaccharides	1059:1094	the prebiotic fructooligosaccharides (FOS)	1059:1100	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	4	83	dep	Escherichia	526:536	arg1	E.					539:540	E.	539:540	E.	539:540	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	3	84	theme	relevant	408:415	arg1	strains					438:444	clinically relevant infectious bacterial strains	397:444	clinically relevant infectious bacterial strains	397:444	The present study characterizes head-to-head the in-vitro pathogen growth inhibition of clinically relevant infectious bacterial strains by different types of probiotics and a synbiotic.					
32403297	4	85	theme	EPEC	548:551	arg1	Shigella					554:561	Shigella	554:561	Shigella (Sh.)	554:567	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	4	85	theme	EPEC	548:551	arg1	inhibition					512:521	In-vitro growth inhibition	496:521	In-vitro growth inhibition of Escherichia (E.) coli EPEC	496:551	In-vitro growth inhibition of Escherichia (E.) coli EPEC, Shigella (Sh.)					
32403297	7	86	contain	containing	817:826	arg2	17938					885:889	L. reuteri DSM 17938	870:889	L. reuteri DSM 17938	870:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	86	contain	containing	817:826	arg1	probiotics					806:815	bacterial mono strain probiotics	784:815	bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938	784:889	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	86	contain	containing	817:826	arg2	Lactobacillus					835:847	Lactobacillus	835:847	Lactobacillus (L.) rhamnosus GG	835:865	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	7	86	contain	containing	817:826	arg2	L.					850:851	L.	850:851	L.	850:851	boulardii, bacterial mono strain probiotics containing either Lactobacillus (L.) rhamnosus GG or L. reuteri DSM 17938, a multi strain probiotic containing three L. rhamnosus strains (E/N, Oxy, Pen), and a multi strain synbiotic containing nine different probiotic bacterial strains and the prebiotic fructooligosaccharides (FOS).					
32403297	6	87	theme	mono	719:722	arg1	strain					724:729	a yeast mono strain probiotic	711:739	a yeast mono strain probiotic containing Saccharomyces (Sac.)	711:771	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	6	87	theme	mono	719:722	arg1	products					697:704	Investigated products	684:704	Investigated products	684:704	Investigated products were a yeast mono strain probiotic containing Saccharomyces (Sac.)					
32403297	10	88	theme	probiotic	1431:1439	arg1	microorganisms					1441:1454	probiotic microorganisms	1431:1454	probiotic microorganisms	1431:1454	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
32403297	9	89	theme	strain	1277:1282	arg1	synbiotic					1284:1292	the multi strain synbiotic	1267:1292	the multi strain synbiotic	1267:1292	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	9	90	dep	L.	1162:1163	arg1	GG					1175:1176	L. rhamnosus GG	1162:1176	L. rhamnosus GG	1162:1176	boulardii and L. rhamnosus GG, medium by L. reuteri DSM 17938 and the L. rhamnosus E/N, Oxy, Pen mixture and strong by the multi strain synbiotic.					
32403297	10	91	theme	selection	1476:1484	arg1	process					1486:1492	the selection process	1472:1492	the selection process of products for further clinical evaluation	1472:1536	Head-to-head in-vitro pathogen growth inhibition experiments can be used to differentiate products from different categories containing probiotic microorganisms and can support the selection process of products for further clinical evaluation.					
33537985	12	0	theme	egg	1757:1759	arg1	yolk					1761:1764	egg yolk	1757:1764	egg yolk	1757:1764	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	11	1	theme	chitosan	1583:1590	arg1	addition					1592:1599	chitosan addition	1583:1599	chitosan addition	1583:1599	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	7	2	theme	chitosan	1074:1081	arg1	concentration					1083:1095	a chitosan concentration	1072:1095	a chitosan concentration of 2 g kg-1 (w/w)	1072:1113	At a chitosan concentration of 2 g kg-1 (w/w), the composite emulsion possesses the best stability.					
33537985	3	3	theme	addition	440:447	arg1	sequence					449:456	chitosan addition sequence	431:456	chitosan addition sequence	431:456	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	9	4	theme	oxidative	1367:1375	arg1	stability					1377:1385	poorer oxidative stability	1360:1385	poorer oxidative stability	1360:1385	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	10	5	theme	emulsion	1519:1526	arg1	system					1528:1533	the emulsion system	1515:1533	the emulsion system	1515:1533	In combination with chitosan it also displayed a negative effect on the oxidative stability of the emulsion system.					
33537985	11	6	theme	chemical	1621:1628	arg1	stability					1630:1638	the physical and chemical stability	1604:1638	the physical and chemical stability of emulsions prepared with egg yolk hydrolysates	1604:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	3	7	theme	chitosan	431:438	arg1	sequence					449:456	chitosan addition sequence	431:456	chitosan addition sequence	431:456	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	11	8	dep	CONCLUSION	1536:1545	arg1	revealed					1560:1567	revealed	1560:1567	revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates	1560:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	4	9	theme	RESULTS	611:617	arg1	sequence					628:635	RESULTS Stacking sequence	611:635	RESULTS Stacking sequence of multilayer materials	611:659	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	11	10	theme	physical	1608:1615	arg1	stability					1630:1638	the physical and chemical stability	1604:1638	the physical and chemical stability of emulsions prepared with egg yolk hydrolysates	1604:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	3	11	from	effect	421:426	arg1	properties					491:500	emulsifying properties	479:500	emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates	479:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	1	12	theme	natural	156:162	arg1	emulsifier					164:173	a natural emulsifier	154:173	a natural emulsifier	154:173	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	6	13	theme	Little	931:936	arg1	addition					947:954	Little chitosan addition	931:954	Little chitosan addition (0.5 g × kg-1 , w/w)	931:975	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	5	14	dep	emulsions	730:738	arg1	dominate					765:772	dominate	765:772	dominate at the interface (functioning on reducing interfacial tension)	765:835	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	5	14	dep	emulsions	730:738	arg1	layered					851:857	layered	851:857	layered on the surface (providing steric hindrance)	851:901	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	0	15	theme	thermal	97:103	arg1	stability					119:127	creaming, thermal and oxidative stability	87:127	creaming, thermal and oxidative stability	87:127	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	0	16	theme	oxidative	109:117	arg1	stability					119:127	creaming, thermal and oxidative stability	87:127	creaming, thermal and oxidative stability	87:127	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	3	17	theme	oxidation	545:553	arg1	resistance					555:564	oxidation resistance	545:564	oxidation resistance	545:564	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	5	18	theme	interfacial	816:826	arg1	tension					828:834	interfacial tension	816:834	interfacial tension	816:834	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	5	19	with	emulsions	730:738	arg1	yolk					749:752	egg yolk	745:752	egg yolk	745:752	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	3	20	theme	egg	570:572	arg1	hydrolysates					579:590	egg yolk hydrolysates	570:590	egg yolk hydrolysates	570:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	5	21	theme	steric	885:890	arg1	hindrance					892:900	steric hindrance	885:900	steric hindrance	885:900	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	0	22	from	study	78:82	arg1	stability					119:127	creaming, thermal and oxidative stability	87:127	creaming, thermal and oxidative stability	87:127	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	4	23	theme	multilayer	640:649	arg1	materials					651:659	multilayer materials	640:659	multilayer materials	640:659	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	5	24	theme	composite	720:728	arg1	emulsions					730:738	The composite emulsions	716:738	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance)	716:901	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	1	25	theme	BACKGROUND	130:139	arg1	yolk					145:148	BACKGROUND Egg yolk	130:148	BACKGROUND Egg yolk	130:148	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	6	26	dep	addition	947:954	arg1	0.5 g × kg-1					957:968	0.5 g × kg-1	957:968	0.5 g × kg-1	957:968	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	6	26	dep	addition	947:954	arg1	w/w					972:974	w/w	972:974	w/w	972:974	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	0	27	theme	hydrolysates	64:75	arg1	properties					41:50	the emulsifying properties	25:50	the emulsifying properties of egg yolk hydrolysates	25:75	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	2	28	from	application	266:276	arg1	system					309:314	an oil-in-water system	293:314	an oil-in-water system	293:314	However, the application of egg yolk in an oil-in-water system is still limited due to poor emulsifying stability.					
33537985	0	29	from	Influence	0:8	arg1	properties					41:50	the emulsifying properties	25:50	the emulsifying properties of egg yolk hydrolysates	25:75	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	13	30	theme	Chemical	1851:1858	arg1	Industry					1860:1867	Chemical Industry	1851:1867	Chemical Industry	1851:1867	© 2021 Society of Chemical Industry.					
33537985	3	31	dep	properties	491:500	arg1	creaming					503:510	creaming	503:510	creaming stability, thermal tolerance and oxidation resistance	503:564	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	0	32	theme	creaming	87:94	arg1	stability					119:127	creaming, thermal and oxidative stability	87:127	creaming, thermal and oxidative stability	87:127	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	5	33	theme	egg	745:747	arg1	yolk					749:752	egg yolk	745:752	egg yolk	745:752	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	4	34	theme	composite	695:703	arg1	emulsions					705:713	composite emulsions	695:713	composite emulsions	695:713	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	9	35	theme	egg	1276:1278	arg1	yolk					1280:1283	Hydrolyzed egg yolk	1265:1283	Hydrolyzed egg yolk	1265:1283	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	6	36	theme	emulsion	1013:1020	arg1	droplets					1022:1029	emulsion droplets	1013:1029	emulsion droplets	1013:1029	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	3	37	theme	egg	407:409	arg1	yolk					411:414	egg yolk	407:414	egg yolk	407:414	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	0	38	theme	chitosan	13:20	arg1	Influence					0:8	Influence	0:8	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.	0:128	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	5	39	theme	better	913:918	arg1	stability					920:928	better stability	913:928	better stability	913:928	The composite emulsions with egg yolk hydrolysis dominate at the interface (functioning on reducing interfacial tension), and chitosan layered on the surface (providing steric hindrance) displayed better stability.					
33537985	10	40	with	combination	1423:1433	arg1	chitosan					1440:1447	chitosan	1440:1447	chitosan	1440:1447	In combination with chitosan it also displayed a negative effect on the oxidative stability of the emulsion system.					
33537985	0	41	theme	egg	55:57	arg1	hydrolysates					64:75	egg yolk hydrolysates	55:75	egg yolk hydrolysates	55:75	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	2	42	theme	emulsifying	345:355	arg1	stability					357:365	poor emulsifying stability	340:365	poor emulsifying stability	340:365	However, the application of egg yolk in an oil-in-water system is still limited due to poor emulsifying stability.					
33537985	3	43	theme	hydrolysates	579:590	arg1	properties					491:500	emulsifying properties	479:500	emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates	479:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	9	44	theme	better	1324:1329	arg1	resistance					1339:1348	better thermal resistance	1324:1348	better thermal resistance	1324:1348	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	12	45	theme	water-abundant	1786:1799	arg1	systems					1806:1812	water-abundant food systems	1786:1812	water-abundant food systems such as beverages	1786:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	12	45	theme	water-abundant	1786:1799	arg1	beverages					1822:1830	beverages	1822:1830	beverages	1822:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	12	46	from	emulsifier	1772:1781	arg1	systems					1806:1812	water-abundant food systems	1786:1812	water-abundant food systems such as beverages	1786:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	12	46	from	emulsifier	1772:1781	arg1	beverages					1822:1830	beverages	1822:1830	beverages	1822:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	7	47	contain	possesses	1139:1147	arg2	stability					1158:1166	the best stability	1149:1166	the best stability	1149:1166	At a chitosan concentration of 2 g kg-1 (w/w), the composite emulsion possesses the best stability.					
33537985	7	47	contain	possesses	1139:1147	arg1	emulsion					1130:1137	the composite emulsion	1116:1137	the composite emulsion	1116:1137	At a chitosan concentration of 2 g kg-1 (w/w), the composite emulsion possesses the best stability.					
33537985	2	48	theme	egg	281:283	arg1	yolk					285:288	egg yolk	281:288	egg yolk	281:288	However, the application of egg yolk in an oil-in-water system is still limited due to poor emulsifying stability.					
33537985	11	49	theme	yolk	1671:1674	arg1	hydrolysates					1676:1687	egg yolk hydrolysates	1667:1687	egg yolk hydrolysates	1667:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	3	50	theme	sequence	449:456	arg1	effect					421:426	the effect	417:426	the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates	417:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	1	51	theme	high-oil-phase	194:207	arg1	salad					246:250	salad	246:250	salad	246:250	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	1	51	theme	high-oil-phase	194:207	arg1	mayonnaise					231:240	mayonnaise	231:240	mayonnaise	231:240	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	1	51	theme	high-oil-phase	194:207	arg1	systems					214:220	high-oil-phase food systems	194:220	high-oil-phase food systems	194:220	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	0	52	dep	Influence	0:8	arg1	study					78:82	study	78:82	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.	0:128	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	12	53	theme	yolk	1761:1764	arg1	application					1742:1752	the application	1738:1752	the application of egg yolk	1738:1764	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	12	53	theme	yolk	1761:1764	arg1	emulsifier					1772:1781	an emulsifier	1769:1781	an emulsifier in water-abundant food systems such as beverages	1769:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	6	54	theme	bridging	1046:1053	arg1	flocculation					1055:1066	bridging flocculation	1046:1066	bridging flocculation	1046:1066	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	4	55	contain	has	661:663	arg2	influence					668:676	an influence	665:676	an influence on properties of composite emulsions	665:713	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	4	55	contain	has	661:663	arg1	sequence					628:635	RESULTS Stacking sequence	611:635	RESULTS Stacking sequence of multilayer materials	611:659	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	2	56	theme	oil-in-water	296:307	arg1	system					309:314	an oil-in-water system	293:314	an oil-in-water system	293:314	However, the application of egg yolk in an oil-in-water system is still limited due to poor emulsifying stability.					
33537985	9	57	theme	poorer	1360:1365	arg1	stability					1377:1385	poorer oxidative stability	1360:1385	poorer oxidative stability	1360:1385	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	11	58	theme	emulsions	1643:1651	arg1	stability					1630:1638	the physical and chemical stability	1604:1638	the physical and chemical stability of emulsions prepared with egg yolk hydrolysates	1604:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	10	59	theme	system	1528:1533	arg1	stability					1502:1510	the oxidative stability	1488:1510	the oxidative stability of the emulsion system	1488:1533	In combination with chitosan it also displayed a negative effect on the oxidative stability of the emulsion system.					
33537985	11	60	theme	addition	1592:1599	arg1	effect					1573:1578	the effect	1569:1578	the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates	1569:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	3	61	theme	emulsifying	479:489	arg1	properties					491:500	emulsifying properties	479:500	emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates	479:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	3	62	theme	concentration	462:474	arg1	effect					421:426	the effect	417:426	the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates	417:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	11	63	from	effect	1573:1578	arg1	stability					1630:1638	the physical and chemical stability	1604:1638	the physical and chemical stability of emulsions prepared with egg yolk hydrolysates	1604:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	8	64	theme	chitosan	1174:1181	arg1	concentration					1183:1195	chitosan concentration	1174:1195	chitosan concentration	1174:1195	When chitosan concentration was higher than 2 g kg-1 (w/w), depletion flocculation would occur.					
33537985	9	65	theme	natural	1402:1408	arg1	yolk					1414:1417	natural egg yolk	1402:1417	natural egg yolk	1402:1417	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	6	66	theme	chitosan	938:945	arg1	addition					947:954	Little chitosan addition	931:954	Little chitosan addition (0.5 g × kg-1 , w/w)	931:975	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	4	67	theme	Stacking	619:626	arg1	sequence					628:635	RESULTS Stacking sequence	611:635	RESULTS Stacking sequence of multilayer materials	611:659	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	9	68	theme	egg	1410:1412	arg1	yolk					1414:1417	natural egg yolk	1402:1417	natural egg yolk	1402:1417	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	3	69	theme	thermal	523:529	arg1	tolerance					531:539	thermal tolerance	523:539	thermal tolerance	523:539	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	0	70	theme	emulsifying	29:39	arg1	properties					41:50	the emulsifying properties	25:50	the emulsifying properties of egg yolk hydrolysates	25:75	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	0	71	theme	yolk	59:62	arg1	hydrolysates					64:75	egg yolk hydrolysates	55:75	egg yolk hydrolysates	55:75	Influence of chitosan on the emulsifying properties of egg yolk hydrolysates: study on creaming, thermal and oxidative stability.					
33537985	7	72	theme	best	1153:1156	arg1	stability					1158:1166	the best stability	1149:1166	the best stability	1149:1166	At a chitosan concentration of 2 g kg-1 (w/w), the composite emulsion possesses the best stability.					
33537985	1	73	theme	Egg	141:143	arg1	yolk					145:148	BACKGROUND Egg yolk	130:148	BACKGROUND Egg yolk	130:148	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	4	74	theme	materials	651:659	arg1	sequence					628:635	RESULTS Stacking sequence	611:635	RESULTS Stacking sequence of multilayer materials	611:659	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	10	75	from	effect	1478:1483	arg1	stability					1502:1510	the oxidative stability	1488:1510	the oxidative stability of the emulsion system	1488:1533	In combination with chitosan it also displayed a negative effect on the oxidative stability of the emulsion system.					
33537985	7	76	theme	composite	1120:1128	arg1	emulsion					1130:1137	the composite emulsion	1116:1137	the composite emulsion	1116:1137	At a chitosan concentration of 2 g kg-1 (w/w), the composite emulsion possesses the best stability.					
33537985	9	77	dep	emulsions	1304:1312	arg1	possessed					1314:1322	possessed	1314:1322	possessed better thermal resistance	1314:1348	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	9	77	dep	emulsions	1304:1312	arg1	stability					1377:1385	poorer oxidative stability	1360:1385	poorer oxidative stability	1360:1385	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	3	78	theme	emulsifying	383:393	arg1	capacity					395:402	the emulsifying capacity	379:402	the emulsifying capacity of egg yolk	379:414	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	9	79	theme	Hydrolyzed	1265:1274	arg1	yolk					1280:1283	Hydrolyzed egg yolk	1265:1283	Hydrolyzed egg yolk	1265:1283	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	6	80	theme	droplets	1022:1029	arg1	dispersion					999:1008	the dispersion	995:1008	the dispersion of emulsion droplets	995:1029	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	4	81	theme	emulsions	705:713	arg1	properties					681:690	properties	681:690	properties of composite emulsions	681:713	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	3	82	theme	yolk	411:414	arg1	capacity					395:402	the emulsifying capacity	379:402	the emulsifying capacity of egg yolk	379:414	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	7	83	theme	2 g kg-1	1100:1107	arg1	concentration					1083:1095	a chitosan concentration	1072:1095	a chitosan concentration of 2 g kg-1 (w/w)	1072:1113	At a chitosan concentration of 2 g kg-1 (w/w), the composite emulsion possesses the best stability.					
33537985	9	84	theme	composite	1294:1302	arg1	emulsions					1304:1312	composite emulsions	1294:1312	composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk	1294:1417	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	3	85	theme	yolk	574:577	arg1	hydrolysates					579:590	egg yolk hydrolysates	570:590	egg yolk hydrolysates	570:590	To improve the emulsifying capacity of egg yolk, the effect of chitosan addition sequence and concentration on emulsifying properties (creaming stability, thermal tolerance and oxidation resistance) of egg yolk hydrolysates were investigated.					
33537985	2	86	theme	poor	340:343	arg1	stability					357:365	poor emulsifying stability	340:365	poor emulsifying stability	340:365	However, the application of egg yolk in an oil-in-water system is still limited due to poor emulsifying stability.					
33537985	12	87	theme	food	1801:1804	arg1	systems					1806:1812	water-abundant food systems	1786:1812	water-abundant food systems such as beverages	1786:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	12	87	theme	food	1801:1804	arg1	beverages					1822:1830	beverages	1822:1830	beverages	1822:1830	The results could provide guidance on expanding the application of egg yolk as an emulsifier in water-abundant food systems such as beverages.					
33537985	10	88	theme	negative	1469:1476	arg1	effect					1478:1483	a negative effect	1467:1483	a negative effect on the oxidative stability of the emulsion system	1467:1533	In combination with chitosan it also displayed a negative effect on the oxidative stability of the emulsion system.					
33537985	2	89	theme	yolk	285:288	arg1	application					266:276	the application	262:276	the application of egg yolk in an oil-in-water system	262:314	However, the application of egg yolk in an oil-in-water system is still limited due to poor emulsifying stability.					
33537985	9	90	theme	thermal	1331:1337	arg1	resistance					1339:1348	better thermal resistance	1324:1348	better thermal resistance	1324:1348	Hydrolyzed egg yolk prepared composite emulsions possessed better thermal resistance, but with poorer oxidative stability as compared to natural egg yolk.					
33537985	11	91	theme	egg	1667:1669	arg1	hydrolysates					1676:1687	egg yolk hydrolysates	1667:1687	egg yolk hydrolysates	1667:1687	CONCLUSION The research revealed the effect of chitosan addition on the physical and chemical stability of emulsions prepared with egg yolk hydrolysates.					
33537985	4	92	from	influence	668:676	arg1	properties					681:690	properties	681:690	properties of composite emulsions	681:713	RESULTS Stacking sequence of multilayer materials has an influence on properties of composite emulsions.					
33537985	1	93	used	used	186:189	arg2	yolk					145:148	BACKGROUND Egg yolk	130:148	BACKGROUND Egg yolk	130:148	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	6	94	theme	flocculation	1055:1066	arg1	result					1036:1041	a result	1034:1041	a result of bridging flocculation	1034:1066	Little chitosan addition (0.5 g × kg-1 , w/w) was unhelpful for the dispersion of emulsion droplets as a result of bridging flocculation.					
33537985	10	95	theme	oxidative	1492:1500	arg1	stability					1502:1510	the oxidative stability	1488:1510	the oxidative stability of the emulsion system	1488:1533	In combination with chitosan it also displayed a negative effect on the oxidative stability of the emulsion system.					
33537985	1	96	theme	food	209:212	arg1	salad					246:250	salad	246:250	salad	246:250	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	1	96	theme	food	209:212	arg1	mayonnaise					231:240	mayonnaise	231:240	mayonnaise	231:240	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33537985	1	96	theme	food	209:212	arg1	systems					214:220	high-oil-phase food systems	194:220	high-oil-phase food systems	194:220	BACKGROUND Egg yolk, as a natural emulsifier, is widely used in high-oil-phase food systems, such as mayonnaise and salad.					
33535111	2	0	theme	dietary	437:443	arg1	fiber					445:449	dietary fiber	437:449	dietary fiber	437:449	The results illustrated that malting resulted in decreases of starch, protein, fat and ash, while it increased dietary fiber, carbohydrate and energy.					
33535111	7	1	theme	technological	1201:1213	arg1	performance					1215:1225	technological performance	1201:1225	technological performance	1201:1225	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	7	2	theme	cookies	1241:1247	arg1	value					1191:1195	nutrition value	1181:1195	nutrition value	1181:1195	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	7	2	theme	cookies	1241:1247	arg1	performance					1215:1225	technological performance	1201:1225	technological performance	1201:1225	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	6	3	theme	WMF	989:991	arg1	incorporation					972:984	The incorporation	968:984	The incorporation of WMF	968:991	The incorporation of WMF induced a distorted "honey-like" comb structure to the cookies.					
33535111	0	4	theme	antioxidant	64:74	arg1	properties					76:85	the compositional, functional and antioxidant properties	30:85	the compositional, functional and antioxidant properties of whole wheat malt	30:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	1	5	theme	cookie	310:315	arg1	quality					317:323	cookie quality	310:323	cookie quality	310:323	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	7	6	theme	physicochemical	1116:1130	arg1	properties					1144:1153	the physicochemical, functional properties	1112:1153	the physicochemical, functional properties of WMF	1112:1160	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	4	7	from	water	648:652	arg1	WMF					657:659	WMF	657:659	WMF	657:659	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	1	8	theme	whole	253:257	arg1	WMF					277:279	WMF	277:279	WMF	277:279	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	8	theme	whole	253:257	arg1	flour					270:274	whole wheat malt flour	253:274	whole wheat malt flour (WMF)	253:280	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	0	9	theme	whole	90:94	arg1	malt					102:105	whole wheat malt	90:105	whole wheat malt	90:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	0	10	from	Effect	0:5	arg1	cookie-making					137:149	cookie-making	137:149	cookie-making	137:149	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	0	10	from	Effect	0:5	arg1	properties					76:85	the compositional, functional and antioxidant properties	30:85	the compositional, functional and antioxidant properties of whole wheat malt	30:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	4	11	theme	malt-based	853:862	arg1	cookies					864:870	malt-based cookies	853:870	malt-based cookies	853:870	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	12	theme	antioxidant	732:742	arg1	capacity					744:751	antioxidant capacity	732:751	antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP)	732:816	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	5	13	theme	tousy	932:936	arg1	structure					938:946	an irregular tousy structure	919:946	an irregular tousy structure	919:946	Flours changed from an integrated granular to an irregular tousy structure during germination.					
33535111	1	14	theme	wheat	259:263	arg1	WMF					277:279	WMF	277:279	WMF	277:279	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	14	theme	wheat	259:263	arg1	flour					270:274	whole wheat malt flour	253:274	whole wheat malt flour (WMF)	253:280	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	7	15	theme	nutrition	1181:1189	arg1	value					1191:1195	nutrition value	1181:1195	nutrition value	1181:1195	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	3	16	theme	emulsifying	492:502	arg1	ability					516:522	emulsifying and foaming ability	492:522	emulsifying and foaming ability	492:522	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	3	16	theme	emulsifying	492:502	arg1	hydration					481:489	Gel hydration	477:489	Gel hydration	477:489	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	1	17	theme	malt	265:268	arg1	WMF					277:279	WMF	277:279	WMF	277:279	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	17	theme	malt	265:268	arg1	flour					270:274	whole wheat malt flour	253:274	whole wheat malt flour (WMF)	253:280	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	0	18	theme	malt	102:105	arg1	properties					76:85	the compositional, functional and antioxidant properties	30:85	the compositional, functional and antioxidant properties of whole wheat malt	30:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	7	19	theme	WMF	1158:1160	arg1	properties					1144:1153	the physicochemical, functional properties	1112:1153	the physicochemical, functional properties of WMF	1112:1160	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	2	20	theme	starch	388:393	arg1	decreases					375:383	decreases	375:383	decreases of starch, protein, fat and ash	375:415	The results illustrated that malting resulted in decreases of starch, protein, fat and ash, while it increased dietary fiber, carbohydrate and energy.					
33535111	3	21	theme	Gel	477:479	arg1	ability					516:522	emulsifying and foaming ability	492:522	emulsifying and foaming ability	492:522	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	3	21	theme	Gel	477:479	arg1	hydration					481:489	Gel hydration	477:489	Gel hydration	477:489	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	3	21	theme	Gel	477:479	arg1	viscosity					533:541	pasting viscosity	525:541	pasting viscosity	525:541	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	6	22	theme	comb	1026:1029	arg1	structure					1031:1039	a distorted "honey-like" comb structure	1001:1039	a distorted "honey-like" comb structure to the cookies	1001:1054	The incorporation of WMF induced a distorted "honey-like" comb structure to the cookies.					
33535111	0	23	theme	wheat	96:100	arg1	malt					102:105	whole wheat malt	90:105	whole wheat malt	90:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	2	24	theme	fat	405:407	arg1	decreases					375:383	decreases	375:383	decreases of starch, protein, fat and ash	375:415	The results illustrated that malting resulted in decreases of starch, protein, fat and ash, while it increased dietary fiber, carbohydrate and energy.					
33535111	1	25	theme	flour	270:274	arg1	functionality					236:248	functionality	236:248	functionality of whole wheat malt flour (WMF)	236:280	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	25	theme	flour	270:274	arg1	changes					224:230	compositional changes	210:230	compositional changes	210:230	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	7	26	theme	controlled	1071:1080	arg1	germination					1082:1092	controlled germination	1071:1092	controlled germination	1071:1092	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	4	27	theme	bound	626:630	arg1	water					648:652	Both bound and immobilized water	621:652	Both bound and immobilized water in WMF	621:659	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	0	28	theme	time	22:25	arg1	Effect					0:5	Effect	0:5	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt	0:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	0	28	theme	time	22:25	arg1	evaluation					123:132	its end-use evaluation	111:132	its end-use evaluation in cookie-making	111:149	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	5	29	theme	irregular	922:930	arg1	structure					938:946	an irregular tousy structure	919:946	an irregular tousy structure	919:946	Flours changed from an integrated granular to an irregular tousy structure during germination.					
33535111	0	30	theme	germination	10:20	arg1	time					22:25	germination time	10:25	germination time	10:25	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	3	31	theme	foaming	508:514	arg1	ability					516:522	emulsifying and foaming ability	492:522	emulsifying and foaming ability	492:522	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	3	31	theme	foaming	508:514	arg1	hydration					481:489	Gel hydration	477:489	Gel hydration	477:489	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	1	32	theme	germination	190:200	arg1	time					202:205	germination time	190:205	germination time	190:205	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	3	33	theme	germination	608:618	arg1	2 days					598:603	the first 2 days	588:603	the first 2 days of germination	588:618	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	1	34	theme	time	202:205	arg1	influence					297:305	its influence	293:305	the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality	176:323	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	34	theme	time	202:205	arg1	effect					180:185	the effect	176:185	the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality	176:323	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	0	35	theme	end-use	115:121	arg1	evaluation					123:132	its end-use evaluation	111:132	its end-use evaluation in cookie-making	111:149	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	3	36	theme	first	592:596	arg1	2 days					598:603	the first 2 days	588:603	the first 2 days of germination	588:618	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	4	37	theme	compounds	794:802	arg1	capacity					744:751	antioxidant capacity	732:751	antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP)	732:816	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	38	dep	compounds	794:802	arg1	HPP					813:815	HPP	813:815	HPP	813:815	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	38	dep	compounds	794:802	arg1	EPP					805:807	EPP	805:807	EPP	805:807	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	38	dep	compounds	794:802	arg1	compounds					794:802	extractable and hydrolyzable phenolic compounds	756:802	extractable and hydrolyzable phenolic compounds (EPP and HPP)	756:816	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	1	39	from	influence	297:305	arg1	quality					317:323	cookie quality	310:323	cookie quality	310:323	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	39	from	influence	297:305	arg1	changes					224:230	compositional changes	210:230	compositional changes	210:230	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	39	from	influence	297:305	arg1	functionality					236:248	functionality	236:248	functionality of whole wheat malt flour (WMF)	236:280	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	7	40	theme	malt-based	1230:1239	arg1	cookies					1241:1247	malt-based cookies	1230:1247	malt-based cookies	1230:1247	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	3	41	theme	pasting	525:531	arg1	viscosity					533:541	pasting viscosity	525:541	pasting viscosity	525:541	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	3	41	theme	pasting	525:531	arg1	hydration					481:489	Gel hydration	477:489	Gel hydration	477:489	Gel hydration, emulsifying and foaming ability, pasting viscosity decreased significantly, particularly during the first 2 days of germination.					
33535111	0	42	theme	compositional	34:46	arg1	properties					76:85	the compositional, functional and antioxidant properties	30:85	the compositional, functional and antioxidant properties of whole wheat malt	30:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	2	43	theme	ash	413:415	arg1	decreases					375:383	decreases	375:383	decreases of starch, protein, fat and ash	375:415	The results illustrated that malting resulted in decreases of starch, protein, fat and ash, while it increased dietary fiber, carbohydrate and energy.					
33535111	4	44	theme	hydrolyzable	772:783	arg1	HPP					813:815	HPP	813:815	HPP	813:815	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	44	theme	hydrolyzable	772:783	arg1	EPP					805:807	EPP	805:807	EPP	805:807	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	44	theme	hydrolyzable	772:783	arg1	compounds					794:802	extractable and hydrolyzable phenolic compounds	756:802	extractable and hydrolyzable phenolic compounds (EPP and HPP)	756:816	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	45	theme	germination	687:697	arg1	time					699:702	germination time	687:702	germination time	687:702	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	0	46	from	evaluation	123:132	arg1	cookie-making					137:149	cookie-making	137:149	cookie-making	137:149	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	0	46	from	evaluation	123:132	arg1	properties					76:85	the compositional, functional and antioxidant properties	30:85	the compositional, functional and antioxidant properties of whole wheat malt	30:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	4	47	theme	extractable	756:766	arg1	HPP					813:815	HPP	813:815	HPP	813:815	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	47	theme	extractable	756:766	arg1	EPP					805:807	EPP	805:807	EPP	805:807	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	47	theme	extractable	756:766	arg1	compounds					794:802	extractable and hydrolyzable phenolic compounds	756:802	extractable and hydrolyzable phenolic compounds (EPP and HPP)	756:816	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	48	theme	immobilized	636:646	arg1	water					648:652	Both bound and immobilized water	621:652	Both bound and immobilized water in WMF	621:659	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	6	49	theme	distorted	1003:1011	arg1	structure					1031:1039	a distorted "honey-like" comb structure	1001:1039	a distorted "honey-like" comb structure to the cookies	1001:1054	The incorporation of WMF induced a distorted "honey-like" comb structure to the cookies.					
33535111	6	50	theme	honey-like	1014:1023	arg1	structure					1031:1039	a distorted "honey-like" comb structure	1001:1039	a distorted "honey-like" comb structure to the cookies	1001:1054	The incorporation of WMF induced a distorted "honey-like" comb structure to the cookies.					
33535111	4	51	theme	phenolic	785:792	arg1	HPP					813:815	HPP	813:815	HPP	813:815	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	51	theme	phenolic	785:792	arg1	EPP					805:807	EPP	805:807	EPP	805:807	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	4	51	theme	phenolic	785:792	arg1	compounds					794:802	extractable and hydrolyzable phenolic compounds	756:802	extractable and hydrolyzable phenolic compounds (EPP and HPP)	756:816	Both bound and immobilized water in WMF decreased with increasing germination time while the concentration and antioxidant capacity of extractable and hydrolyzable phenolic compounds (EPP and HPP) increased significantly in WMF and malt-based cookies.					
33535111	1	52	from	effect	180:185	arg1	functionality					236:248	functionality	236:248	functionality of whole wheat malt flour (WMF)	236:280	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	52	from	effect	180:185	arg1	quality					317:323	cookie quality	310:323	cookie quality	310:323	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	1	52	from	effect	180:185	arg1	changes					224:230	compositional changes	210:230	compositional changes	210:230	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	0	53	theme	functional	49:58	arg1	properties					76:85	the compositional, functional and antioxidant properties	30:85	the compositional, functional and antioxidant properties of whole wheat malt	30:105	Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making.					
33535111	2	54	theme	protein	396:402	arg1	decreases					375:383	decreases	375:383	decreases of starch, protein, fat and ash	375:415	The results illustrated that malting resulted in decreases of starch, protein, fat and ash, while it increased dietary fiber, carbohydrate and energy.					
33535111	1	55	theme	compositional	210:222	arg1	changes					224:230	compositional changes	210:230	compositional changes	210:230	This study investigated the effect of germination time on compositional changes and functionality of whole wheat malt flour (WMF) as well as its influence on cookie quality.					
33535111	7	56	dep	physicochemical	1116:1130	arg1	functional					1133:1142	functional	1133:1142	functional	1133:1142	Conclusively, controlled germination not only improves the physicochemical, functional properties of WMF but also increases nutrition value and technological performance of malt-based cookies.					
33535111	6	57	theme	"	1024:1024	arg1	structure					1031:1039	a distorted "honey-like" comb structure	1001:1039	a distorted "honey-like" comb structure to the cookies	1001:1054	The incorporation of WMF induced a distorted "honey-like" comb structure to the cookies.					
33183445	9	0	theme	certain	1635:1641	arg1	function					1661:1668	a certain sustained release function	1633:1668	a certain sustained release function	1633:1668	At the same time, the material also has a certain sustained release function.					
33183445	8	1	theme	study	1434:1438	arg1	purpose					1418:1424	The purpose	1414:1424	The purpose of this study	1414:1438	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	1	2	theme	raw	223:225	arg1	carrier					157:163	a solid carrier	149:163	a solid carrier	149:163	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	1	2	theme	raw	223:225	arg1	materials					227:235	the main raw materials	214:235	the main raw materials	214:235	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	9	3	theme	release	1653:1659	arg1	function					1661:1668	a certain sustained release function	1633:1668	a certain sustained release function	1633:1668	At the same time, the material also has a certain sustained release function.					
33183445	6	4	theme	Klotho	1130:1135	arg1	group					1137:1141	the Klotho group	1126:1141	the Klotho group alone	1126:1147	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	7	5	theme	ulcers	1302:1307	arg1	healing					1282:1288	the healing	1278:1288	the healing of diabetic ulcers	1278:1307	It is proved that both Klotho and BMSCs can help the healing of diabetic ulcers, but BMSCs alone cannot survive in harsh environments, and it is difficult to play a normal repair role.					
33183445	4	6	theme	PLGA	759:762	arg1	film					764:767	the PLGA film	755:767	the PLGA film	755:767	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	5	7	with	mice	920:923	arg1	ulcers					939:944	diabetic ulcers	930:944	diabetic ulcers	930:944	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	8	8	theme	high	1511:1514	arg1	conditions					1524:1533	high glucose conditions	1511:1533	high glucose conditions	1511:1533	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	4	9	theme	material	814:821	arg1	surface					799:805	the surface	795:805	the surface of the material	795:821	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	8	10	from	BMSCs	1580:1584	arg1	it					1589:1590	it	1589:1590	it	1589:1590	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	0	11	theme	Infection	118:126	arg1	Treatment					79:87	the Treatment	75:87	the Treatment of Diabetic Foot Ulcer Wound Infection	75:126	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	2	12	theme	PLGA	487:490	arg1	nanofibers					492:501	PLGA nanofibers	487:501	PLGA nanofibers	487:501	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	12	13	theme	ulcer	2088:2092	arg1	areas					2094:2098	diabetic ulcer areas	2079:2098	diabetic ulcer areas	2079:2098	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	10	14	theme	BMSCs	1756:1760	arg1	migration					1743:1751	migration	1743:1751	migration	1743:1751	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	10	14	theme	BMSCs	1756:1760	arg1	proliferation					1725:1737	proliferation	1725:1737	proliferation	1725:1737	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	1	15	theme	protein	346:352	arg1	stimulation					324:334	the stimulation	320:334	the stimulation of Klotho protein	320:352	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	6	16	theme	best	1093:1096	arg1	effect					1106:1111	the best healing effect	1089:1111	the best healing effect	1089:1111	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	6	17	theme	other	1160:1164	arg1	groups					1172:1177	the other three groups	1156:1177	the other three groups	1156:1177	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	3	18	theme	PLGA	522:525	arg1	nanofibers					527:536	PLGA nanofibers	522:536	PLGA nanofibers	522:536	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	3	18	theme	PLGA	522:525	arg1	material					568:575	a controlled release material	547:575	a controlled release material	547:575	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	8	19	theme	suitable	1550:1557	arg1	carrier					1559:1565	a suitable carrier	1548:1565	a suitable carrier for planting BMSCs on it	1548:1590	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	10	20	theme	high	1776:1779	arg1	conditions					1789:1798	high glucose conditions	1776:1798	high glucose conditions	1776:1798	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	3	21	theme	release	560:566	arg1	nanofibers					527:536	PLGA nanofibers	522:536	PLGA nanofibers	522:536	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	3	21	theme	release	560:566	arg1	material					568:575	a controlled release material	547:575	a controlled release material	547:575	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	4	22	theme	Klotho	875:880	arg1	protein					882:888	the inner Klotho protein	865:888	the inner Klotho protein	865:888	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	6	23	theme	Klotho+BMSCs	1061:1072	arg1	group					1074:1078	the Klotho+BMSCs group	1057:1078	the Klotho+BMSCs group	1057:1078	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	3	24	from	time	516:519	arg1	nanofibers					527:536	PLGA nanofibers	522:536	PLGA nanofibers	522:536	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	3	24	from	time	516:519	arg1	material					568:575	a controlled release material	547:575	a controlled release material	547:575	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	10	25	theme	ECs	1766:1768	arg1	migration					1743:1751	migration	1743:1751	migration	1743:1751	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	10	25	theme	ECs	1766:1768	arg1	proliferation					1725:1737	proliferation	1725:1737	proliferation	1725:1737	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	5	26	theme	DFU	1003:1005	arg1	healing					1007:1013	DFU healing	1003:1013	DFU healing	1003:1013	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	7	27	theme	normal	1394:1399	arg1	role					1408:1411	a normal repair role	1392:1411	a normal repair role	1392:1411	It is proved that both Klotho and BMSCs can help the healing of diabetic ulcers, but BMSCs alone cannot survive in harsh environments, and it is difficult to play a normal repair role.					
33183445	2	28	theme	suitable	427:434	arg1	environment					452:462	suitable adhesion growth environment	427:462	suitable adhesion growth environment for BMSCs	427:472	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	12	29	theme	artificial	2025:2034	arg1	protein					2043:2049	we artificial Klotho protein	2022:2049	we artificial Klotho protein	2022:2049	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	1	30	theme	solid	151:155	arg1	carrier					157:163	a solid carrier	149:163	a solid carrier	149:163	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	1	30	theme	solid	151:155	arg1	materials					227:235	the main raw materials	214:235	the main raw materials	214:235	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	0	31	theme	Diabetic	92:99	arg1	Infection					118:126	Diabetic Foot Ulcer Wound Infection	92:126	Diabetic Foot Ulcer Wound Infection	92:126	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	5	32	theme	protein	982:988	arg1	effects					964:970	the effects	960:970	the effects of Klotho protein and BMSCs on DFU healing	960:1013	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	0	33	theme	Ulcer	106:110	arg1	Infection					118:126	Diabetic Foot Ulcer Wound Infection	92:126	Diabetic Foot Ulcer Wound Infection	92:126	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	5	34	theme	BMSCs	994:998	arg1	effects					964:970	the effects	960:970	the effects of Klotho protein and BMSCs on DFU healing	960:1013	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	11	35	theme	suitable	1927:1934	arg1	microstructure					1912:1925	a microstructure	1910:1925	a microstructure suitable for BMSCs adhesion	1910:1953	When combined with electrospinning technology to prepare a protein that can release Klotho, it also provides a microstructure suitable for BMSCs adhesion, thereby ensuring that BMSCs can successfully survive.					
33183445	2	36	theme	growth	445:450	arg1	environment					452:462	suitable adhesion growth environment	427:462	suitable adhesion growth environment for BMSCs	427:472	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	0	37	theme	lactic-co-glycolic	5:22	arg1	Poly					0:3	Poly	0:3	Poly(lactic-co-glycolic acid)	0:28	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	0	37	theme	lactic-co-glycolic	5:22	arg1	acid					24:27	lactic-co-glycolic acid	5:27	lactic-co-glycolic acid	5:27	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	11	38	theme	BMSCs	1940:1944	arg1	adhesion					1946:1953	BMSCs adhesion	1940:1953	BMSCs adhesion	1940:1953	When combined with electrospinning technology to prepare a protein that can release Klotho, it also provides a microstructure suitable for BMSCs adhesion, thereby ensuring that BMSCs can successfully survive.					
33183445	12	39	theme	ulcer	2158:2162	arg1	areas					2164:2168	ulcer areas	2158:2168	ulcer areas	2158:2168	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	2	40	theme	adhesion	436:443	arg1	environment					452:462	suitable adhesion growth environment	427:462	suitable adhesion growth environment for BMSCs	427:472	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	0	41	theme	Composite	47:55	arg1	Nanomaterials					57:69	Composite Nanomaterials	47:69	Composite Nanomaterials	47:69	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	5	42	theme	mice	1033:1036	arg1	mice					1033:1036	mice	1033:1036	mice	1033:1036	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	5	42	theme	mice	1033:1036	arg1	groups					1023:1028	five groups	1018:1028	five groups of mice	1018:1036	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	5	43	theme	diabetic	930:937	arg1	ulcers					939:944	diabetic ulcers	930:944	diabetic ulcers	930:944	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	4	44	theme	porous	711:716	arg1	structure					718:726	the nano-scale porous structure	696:726	the nano-scale porous structure provided on the surface of the PLGA film	696:767	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	2	45	theme	technical	400:408	arg1	means					410:414	the main technical means	391:414	the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers	391:501	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	2	45	theme	technical	400:408	arg1	PLGA					383:386	electrospun PLGA	371:386	electrospun PLGA	371:386	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	8	46	theme	Klotho	1473:1478	arg1	protein					1480:1486	Klotho protein	1473:1486	Klotho protein	1473:1486	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	1	47	theme	main	218:221	arg1	carrier					157:163	a solid carrier	149:163	a solid carrier	149:163	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	1	47	theme	main	218:221	arg1	materials					227:235	the main raw materials	214:235	the main raw materials	214:235	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	9	48	theme	sustained	1643:1651	arg1	function					1661:1668	a certain sustained release function	1633:1668	a certain sustained release function	1633:1668	At the same time, the material also has a certain sustained release function.					
33183445	7	49	theme	diabetic	1293:1300	arg1	ulcers					1302:1307	diabetic ulcers	1293:1307	diabetic ulcers	1293:1307	It is proved that both Klotho and BMSCs can help the healing of diabetic ulcers, but BMSCs alone cannot survive in harsh environments, and it is difficult to play a normal repair role.					
33183445	10	50	theme	Klotho	1694:1699	arg1	protein					1701:1707	Klotho protein	1694:1707	Klotho protein	1694:1707	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	1	51	theme	repairing	270:278	arg1	effect					280:285	their repairing effect	264:285	their repairing effect	264:285	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	3	52	theme	same	511:514	arg1	time					516:519	the same time	507:519	the same time	507:519	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	4	53	theme	film	764:767	arg1	surface					744:750	the surface	740:750	the surface of the PLGA film	740:767	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	3	54	theme	long	677:680	arg1	time					682:685	a long time	675:685	a long time	675:685	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	6	55	theme	significant	1186:1196	arg1	difference					1198:1207	no significant difference	1183:1207	no significant difference in healing effects	1183:1226	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	8	56	theme	glucose	1516:1522	arg1	conditions					1524:1533	high glucose conditions	1511:1533	high glucose conditions	1511:1533	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	1	57	theme	Klotho	339:344	arg1	protein					346:352	Klotho protein	339:352	Klotho protein	339:352	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	6	58	theme	healing	1098:1104	arg1	effect					1106:1111	the best healing effect	1089:1111	the best healing effect	1089:1111	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	5	59	theme	Klotho	975:980	arg1	protein					982:988	Klotho protein	975:988	Klotho protein	975:988	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	11	60	theme	electrospinning	1820:1834	arg1	technology					1836:1845	electrospinning technology	1820:1845	electrospinning technology	1820:1845	When combined with electrospinning technology to prepare a protein that can release Klotho, it also provides a microstructure suitable for BMSCs adhesion, thereby ensuring that BMSCs can successfully survive.					
33183445	3	61	theme	controlled	549:558	arg1	nanofibers					527:536	PLGA nanofibers	522:536	PLGA nanofibers	522:536	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	3	61	theme	controlled	549:558	arg1	material					568:575	a controlled release material	547:575	a controlled release material	547:575	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	6	62	contain	had	1179:1181	arg2	difference					1198:1207	no significant difference	1183:1207	no significant difference in healing effects	1183:1226	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	6	62	contain	had	1179:1181	arg1	groups					1172:1177	the other three groups	1156:1177	the other three groups	1156:1177	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	7	63	theme	repair	1401:1406	arg1	role					1408:1411	a normal repair role	1392:1411	a normal repair role	1392:1411	It is proved that both Klotho and BMSCs can help the healing of diabetic ulcers, but BMSCs alone cannot survive in harsh environments, and it is difficult to play a normal repair role.					
33183445	12	64	theme	diabetic	2079:2086	arg1	areas					2094:2098	diabetic ulcer areas	2079:2098	diabetic ulcer areas	2079:2098	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	4	65	theme	inner	869:873	arg1	protein					882:888	the inner Klotho protein	865:888	the inner Klotho protein	865:888	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	12	66	theme	Klotho	2036:2041	arg1	protein					2043:2049	we artificial Klotho protein	2022:2049	we artificial Klotho protein	2022:2049	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	6	67	theme	healing	1212:1218	arg1	effects					1220:1226	healing effects	1212:1226	healing effects	1212:1226	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	0	68	theme	Foot	101:104	arg1	Ulcer					106:110	Foot Ulcer	101:110	Diabetic Foot Ulcer Wound Infection	92:126	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	1	69	theme	ulcer	303:307	arg1	area					309:312	the ulcer area	299:312	the ulcer area under the stimulation of Klotho protein	299:352	In this experiment, a solid carrier was prepared with PLGA, gelatin, and chitosan as the main raw materials, so that BMSCs could exert their repairing effect directly in the ulcer area under the stimulation of Klotho protein.					
33183445	8	70	from	effect	1463:1468	arg1	ECs					1501:1503	ECs	1501:1503	ECs	1501:1503	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	8	70	from	effect	1463:1468	arg1	BMSCs					1491:1495	BMSCs	1491:1495	BMSCs	1491:1495	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33183445	10	71	theme	glucose	1781:1787	arg1	conditions					1789:1798	high glucose conditions	1776:1798	high glucose conditions	1776:1798	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	0	72	theme	Wound	112:116	arg1	Infection					118:126	Diabetic Foot Ulcer Wound Infection	92:126	Diabetic Foot Ulcer Wound Infection	92:126	Poly(lactic-co-glycolic acid)-Chitosan-Gelatin Composite Nanomaterials for the Treatment of Diabetic Foot Ulcer Wound Infection.					
33183445	6	73	from	difference	1198:1207	arg1	effects					1220:1226	healing effects	1212:1226	healing effects	1212:1226	From the results, the Klotho+BMSCs group achieved the best healing effect, followed by the Klotho group alone, while the other three groups had no significant difference in healing effects.					
33183445	10	74	dep	proliferation	1725:1737	arg1	the					1721:1723	the	1721:1723	the	1721:1723	We have concluded that Klotho protein can promote the proliferation and migration of BMSCs and ECs under high glucose conditions.					
33183445	2	75	theme	electrospun	371:381	arg1	means					410:414	the main technical means	391:414	the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers	391:501	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	2	75	theme	electrospun	371:381	arg1	PLGA					383:386	electrospun PLGA	371:386	electrospun PLGA	371:386	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	9	76	contain	has	1629:1631	arg2	function					1661:1668	a certain sustained release function	1633:1668	a certain sustained release function	1633:1668	At the same time, the material also has a certain sustained release function.					
33183445	9	76	contain	has	1629:1631	arg1	material					1615:1622	the material	1611:1622	the material	1611:1622	At the same time, the material also has a certain sustained release function.					
33183445	9	77	theme	same	1600:1603	arg1	time					1605:1608	the same time	1596:1608	the same time	1596:1608	At the same time, the material also has a certain sustained release function.					
33183445	12	78	theme	areas	2164:2168	arg1	healing					2147:2153	healing	2147:2153	healing of ulcer areas	2147:2168	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	5	79	from	effects	964:970	arg1	healing					1007:1013	DFU healing	1003:1013	DFU healing	1003:1013	We applied this composite to mice with diabetic ulcers, and verified the effects of Klotho protein and BMSCs on DFU healing in five groups of mice.					
33183445	12	80	from	angiogenesis	2063:2074	arg1	areas					2094:2098	diabetic ulcer areas	2079:2098	diabetic ulcer areas	2079:2098	In the end, we artificial Klotho protein can promote angiogenesis in diabetic ulcer areas by protecting BMSCs and ECs, thereby promoting healing of ulcer areas.					
33183445	2	81	theme	main	395:398	arg1	means					410:414	the main technical means	391:414	the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers	391:501	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	2	81	theme	main	395:398	arg1	PLGA					383:386	electrospun PLGA	371:386	electrospun PLGA	371:386	We chose to use electrospun PLGA as the main technical means to provide suitable adhesion growth environment for BMSCs by preparing PLGA nanofibers.					
33183445	7	82	theme	harsh	1344:1348	arg1	environments					1350:1361	harsh environments	1344:1361	harsh environments	1344:1361	It is proved that both Klotho and BMSCs can help the healing of diabetic ulcers, but BMSCs alone cannot survive in harsh environments, and it is difficult to play a normal repair role.					
33183445	3	83	theme	Klotho	586:591	arg1	protein					593:599	Klotho protein	586:599	Klotho protein	586:599	At the same time, PLGA nanofibers are also a controlled release material, so that Klotho protein can remain active, thereby achieving the purpose of stimulating BMSCs for a long time.					
33183445	4	84	theme	nano-scale	700:709	arg1	structure					718:726	the nano-scale porous structure	696:726	the nano-scale porous structure provided on the surface of the PLGA film	696:767	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	4	85	from	protein	882:888	arg1	stimulation					848:858	stimulation	848:858	stimulation from the inner Klotho protein	848:888	Through the nano-scale porous structure provided on the surface of the PLGA film, BMSCs can adhere well to the surface of the material and continuously receive stimulation from the inner Klotho protein.					
33183445	8	86	theme	protein	1480:1486	arg1	effect					1463:1468	the effect	1459:1468	the effect of Klotho protein on BMSCs and ECs	1459:1503	The purpose of this study was to investigate the effect of Klotho protein on BMSCs and ECs under high glucose conditions, and to find a suitable carrier for planting BMSCs on it.					
33423558	6	0	theme	histomorphometric	1126:1142	arg1	analyses					1168:1175	The histomorphometric and immunohistochemical analyses	1122:1175	The histomorphometric and immunohistochemical analyses	1122:1175	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	1	1	theme	natural	280:286	arg1	matrices					302:309	natural extracellular matrices	280:309	natural extracellular matrices	280:309	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	7	2	theme	local	1411:1415	arg1	system					1431:1436	a local drug delivery system	1409:1436	a local drug delivery system with good bone induction performance	1409:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	2	theme	local	1411:1415	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	3	3	theme	unconventional	541:554	arg1	hydrogels					569:577	an unconventional dual network hydrogels	538:577	an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs)	538:739	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	1	4	theme	extracellular	288:300	arg1	matrices					302:309	natural extracellular matrices	280:309	natural extracellular matrices	280:309	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	0	5	theme	rat	119:121	arg1	regeneration					129:140	rat skull regeneration	119:140	rat skull regeneration	119:140	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	5	6	theme	crosslinking	1019:1030	arg1	density					1032:1038	higher crosslinking density	1012:1038	higher crosslinking density	1012:1038	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	4	7	theme	bone	885:888	arg1	function					903:910	the bone regeneration function	881:910	the bone regeneration function of the DN hydrogel	881:929	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	5	8	theme	swelling	1051:1058	arg1	degree					1060:1065	the lower swelling degree	1041:1065	the lower swelling degree	1041:1065	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	2	9	theme	single	323:328	arg1	hydrogel					348:355	a single component polymer hydrogel	321:355	a single component polymer hydrogel	321:355	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	2	9	theme	single	323:328	arg1	detrimental					370:380	detrimental	370:380	detrimental	370:380	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	3	10	theme	glycol	610:615	arg1	network					645:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	5	11	theme	resulting	936:944	arg1	hydrogels					963:971	The resulting PEGDA/TCS/T-HNTs hydrogels	932:971	The resulting PEGDA/TCS/T-HNTs hydrogels	932:971	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	5	12	theme	mechanical	991:1000	arg1	property					1002:1009	favourable mechanical property	980:1009	favourable mechanical property	980:1009	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	4	13	theme	morphogenetic	782:794	arg1	protein-2					796:804	bone morphogenetic protein-2	777:804	bone morphogenetic protein-2 (BMP-2)	777:812	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	4	13	theme	morphogenetic	782:794	arg1	BMP-2					807:811	BMP-2	807:811	BMP-2	807:811	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	7	14	theme	induction	1453:1461	arg1	performance					1463:1473	good bone induction performance	1443:1473	good bone induction performance	1443:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	15	with	system	1431:1436	arg1	performance					1463:1473	good bone induction performance	1443:1473	good bone induction performance	1443:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	6	16	theme	excellent	1190:1198	arg1	regeneration					1205:1216	the excellent bone regeneration	1186:1216	the excellent bone regeneration ability	1186:1224	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	7	17	theme	bone	1362:1365	arg1	biomaterial					1380:1390	a new bone regeneration biomaterial	1356:1390	a new bone regeneration biomaterial	1356:1390	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	17	theme	bone	1362:1365	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	1	18	theme	great	206:210	arg1	attention					212:220	great attention	206:220	great attention	206:220	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	6	19	theme	skull	1272:1276	arg1	defect					1278:1283	rat skull defect	1268:1283	rat skull defect	1268:1283	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	0	20	theme	skull	123:127	arg1	regeneration					129:140	rat skull regeneration	119:140	rat skull regeneration	119:140	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	6	21	theme	regeneration	1205:1216	arg1	ability					1218:1224	the excellent bone regeneration ability	1186:1224	the excellent bone regeneration ability	1186:1224	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	1	22	dep	Hydrogel	143:150	arg1	serve					152:156	serve	152:156	serve as bone tissue engineering	152:183	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	0	23	theme	modified	12:19	arg1	glycol					34:39	Halloysites modified polyethylene glycol	0:39	Halloysites modified polyethylene glycol	0:39	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	3	24	theme	diacrylate	617:626	arg1	network					645:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	2	25	theme	mechanical	417:426	arg1	properties					428:437	the weaker mechanical properties	406:437	the weaker mechanical properties	406:437	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	5	26	theme	PEGDA/TCS/T-HNTs	946:961	arg1	hydrogels					963:971	The resulting PEGDA/TCS/T-HNTs hydrogels	932:971	The resulting PEGDA/TCS/T-HNTs hydrogels	932:971	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	2	27	theme	weaker	410:415	arg1	properties					428:437	the weaker mechanical properties	406:437	the weaker mechanical properties	406:437	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	7	28	theme	drug	1417:1420	arg1	system					1431:1436	a local drug delivery system	1409:1436	a local drug delivery system with good bone induction performance	1409:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	28	theme	drug	1417:1420	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	4	29	theme	hydrogel	922:929	arg1	function					903:910	the bone regeneration function	881:910	the bone regeneration function of the DN hydrogel	881:929	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	7	30	theme	delivery	1422:1429	arg1	system					1431:1436	a local drug delivery system	1409:1436	a local drug delivery system with good bone induction performance	1409:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	30	theme	delivery	1422:1429	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	2	31	theme	polymer	340:346	arg1	hydrogel					348:355	a single component polymer hydrogel	321:355	a single component polymer hydrogel	321:355	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	2	31	theme	polymer	340:346	arg1	detrimental					370:380	detrimental	370:380	detrimental	370:380	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	4	32	theme	prepared	834:841	arg1	network					848:854	the prepared dual network	830:854	the prepared dual network (DN)	830:859	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	4	33	dep	network	848:854	arg1	DN					857:858	DN	857:858	DN	857:858	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	0	34	theme	polyethylene	21:32	arg1	glycol					34:39	Halloysites modified polyethylene glycol	0:39	Halloysites modified polyethylene glycol	0:39	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	4	35	theme	DN	919:920	arg1	hydrogel					922:929	the DN hydrogel	915:929	the DN hydrogel	915:929	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	5	36	theme	cell	1099:1102	arg1	ability					1113:1119	cell adhesion ability	1099:1119	cell adhesion ability	1099:1119	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	2	37	theme	component	330:338	arg1	hydrogel					348:355	a single component polymer hydrogel	321:355	a single component polymer hydrogel	321:355	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	2	37	theme	component	330:338	arg1	detrimental					370:380	detrimental	370:380	detrimental	370:380	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	3	38	theme	dual	556:559	arg1	hydrogels					569:577	an unconventional dual network hydrogels	538:577	an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs)	538:739	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	0	39	theme	chitosan	62:69	arg1	hydrogel					86:93	chitosan double network hydrogel	62:93	chitosan double network hydrogel combined with BMP-2 for rat skull regeneration	62:140	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	5	40	theme	favourable	980:989	arg1	property					1002:1009	favourable mechanical property	980:1009	favourable mechanical property	980:1009	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	3	41	theme	ion	681:683	arg1	network					698:704	a thiolated chitosan (TCS) ion crosslinking network	654:704	a thiolated chitosan (TCS) ion crosslinking network	654:704	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	3	42	theme	covalent	636:643	arg1	network					645:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	1	43	theme	good	232:235	arg1	biocompatibility					237:252	their good biocompatibility	226:252	their good biocompatibility	226:252	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	7	44	theme	good	1443:1446	arg1	performance					1463:1473	good bone induction performance	1443:1473	good bone induction performance	1443:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	5	45	theme	lower	1045:1049	arg1	degree					1060:1065	the lower swelling degree	1041:1065	the lower swelling degree	1041:1065	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	4	46	theme	bone	777:780	arg1	protein-2					796:804	bone morphogenetic protein-2	777:804	bone morphogenetic protein-2 (BMP-2)	777:812	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	4	46	theme	bone	777:780	arg1	BMP-2					807:811	BMP-2	807:811	BMP-2	807:811	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	0	47	theme	network	78:84	arg1	hydrogel					86:93	chitosan double network hydrogel	62:93	chitosan double network hydrogel combined with BMP-2 for rat skull regeneration	62:140	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	5	48	theme	excellent	1068:1076	arg1	biocompatibility					1078:1093	excellent biocompatibility	1068:1093	excellent biocompatibility	1068:1093	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	3	49	theme	PEGDA	629:633	arg1	network					645:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	6	50	theme	immunohistochemical	1148:1166	arg1	analyses					1168:1175	The histomorphometric and immunohistochemical analyses	1122:1175	The histomorphometric and immunohistochemical analyses	1122:1175	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	2	51	theme	cell	385:388	arg1	adhesion					390:397	cell adhesion	385:397	cell adhesion	385:397	However, a single component polymer hydrogel is generally detrimental to cell adhesion due to the weaker mechanical properties, which limits their application considerably.					
33423558	0	52	theme	double	71:76	arg1	hydrogel					86:93	chitosan double network hydrogel	62:93	chitosan double network hydrogel combined with BMP-2 for rat skull regeneration	62:140	Halloysites modified polyethylene glycol diacrylate/thiolated chitosan double network hydrogel combined with BMP-2 for rat skull regeneration.					
33423558	4	53	theme	regeneration	890:901	arg1	function					903:910	the bone regeneration function	881:910	the bone regeneration function of the DN hydrogel	881:929	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	4	54	theme	dual	843:846	arg1	network					848:854	the prepared dual network	830:854	the prepared dual network (DN)	830:859	In addition, bone morphogenetic protein-2 (BMP-2) was loaded into the prepared dual network (DN) hydrogel to improve the bone regeneration function of the DN hydrogel.					
33423558	7	55	theme	composite	1319:1327	arg1	biomaterial					1380:1390	a new bone regeneration biomaterial	1356:1390	a new bone regeneration biomaterial	1356:1390	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	55	theme	composite	1319:1327	arg1	system					1431:1436	a local drug delivery system	1409:1436	a local drug delivery system with good bone induction performance	1409:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	55	theme	composite	1319:1327	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	3	56	theme	thiolated	710:718	arg1	T-HNTs					733:738	T-HNTs	733:738	T-HNTs	733:738	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	3	56	theme	thiolated	710:718	arg1	halloysites					720:730	thiolated halloysites	710:730	thiolated halloysites (T-HNTs)	710:739	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	3	57	theme	thiolated	656:664	arg1	TCS					676:678	TCS	676:678	TCS	676:678	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	3	57	theme	thiolated	656:664	arg1	chitosan					666:673	a thiolated chitosan	654:673	a thiolated chitosan (TCS) ion crosslinking network	654:704	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	3	58	theme	polyethylene	597:608	arg1	network					645:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	the polyethylene glycol diacrylate (PEGDA) covalent network	593:651	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	7	59	theme	bone	1448:1451	arg1	performance					1463:1473	good bone induction performance	1443:1473	good bone induction performance	1443:1473	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	6	60	theme	composite	1230:1238	arg1	hydrogel					1240:1247	composite hydrogel	1230:1247	composite hydrogel	1230:1247	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	3	61	theme	crosslinking	685:696	arg1	network					698:704	a thiolated chitosan (TCS) ion crosslinking network	654:704	a thiolated chitosan (TCS) ion crosslinking network	654:704	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	3	62	theme	chitosan	666:673	arg1	network					698:704	a thiolated chitosan (TCS) ion crosslinking network	654:704	a thiolated chitosan (TCS) ion crosslinking network	654:704	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33423558	5	63	theme	adhesion	1104:1111	arg1	ability					1113:1119	cell adhesion ability	1099:1119	cell adhesion ability	1099:1119	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	1	64	theme	tissue	166:171	arg1	engineering					173:183	bone tissue engineering	161:183	bone tissue engineering	161:183	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	7	65	theme	regeneration	1367:1378	arg1	biomaterial					1380:1390	a new bone regeneration biomaterial	1356:1390	a new bone regeneration biomaterial	1356:1390	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	65	theme	regeneration	1367:1378	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	6	66	theme	rat	1268:1270	arg1	defect					1278:1283	rat skull defect	1268:1283	rat skull defect	1268:1283	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	7	67	theme	new	1358:1360	arg1	biomaterial					1380:1390	a new bone regeneration biomaterial	1356:1390	a new bone regeneration biomaterial	1356:1390	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	7	67	theme	new	1358:1360	arg1	scaffold					1329:1336	composite scaffold	1319:1336	composite scaffold	1319:1336	Thus, our results indicated that composite scaffold can be applied as a new bone regeneration biomaterial to be applied as a local drug delivery system with good bone induction performance.					
33423558	1	68	theme	bone	161:164	arg1	engineering					173:183	bone tissue engineering	161:183	bone tissue engineering	161:183	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	1	69	theme	similar	269:275	arg1	structures					258:267	structures	258:267	structures similar to natural extracellular matrices	258:309	Hydrogel serve as bone tissue engineering have lately received great attention for their good biocompatibility and structures similar to natural extracellular matrices.					
33423558	5	70	theme	higher	1012:1017	arg1	density					1032:1038	higher crosslinking density	1012:1038	higher crosslinking density	1012:1038	The resulting PEGDA/TCS/T-HNTs hydrogels showed favourable mechanical property, higher crosslinking density, the lower swelling degree, excellent biocompatibility and cell adhesion ability.					
33423558	6	71	theme	bone	1200:1203	arg1	regeneration					1205:1216	the excellent bone regeneration	1186:1216	the excellent bone regeneration ability	1186:1224	The histomorphometric and immunohistochemical analyses revealed the excellent bone regeneration ability for composite hydrogel after implant into rat skull defect.					
33423558	3	72	theme	network	561:567	arg1	hydrogels					569:577	an unconventional dual network hydrogels	538:577	an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs)	538:739	In an effort to overcome this disadvantage, we adopt an unconventional dual network hydrogels consisting of the polyethylene glycol diacrylate (PEGDA) covalent network, a thiolated chitosan (TCS) ion crosslinking network and thiolated halloysites (T-HNTs) as reinforcing filler.					
33617221	0	0	theme	Near-Infrared	89:101	arg1	Excitation					103:112	Near-Infrared Excitation	89:112	Near-Infrared Excitation	89:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	1	1	theme	photodynamic	356:367	arg1	therapy					369:375	photodynamic therapy	356:375	photodynamic therapy (PDT)	356:381	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	1	1	theme	photodynamic	356:367	arg1	PDT					378:380	PDT	378:380	PDT	378:380	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	4	2	theme	laser	736:740	arg1	excitation					742:751	laser excitation	736:751	laser excitation	736:751	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	1	3	theme	therapy	369:375	arg1	demand					346:351	the high-priority demand	328:351	the high-priority demand of photodynamic therapy (PDT)	328:381	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	1	4	theme	deep	185:188	arg1	imaging					216:222	deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability	185:294	imaging	216:222	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	5	5	theme	tissue	1007:1012	arg1	imaging					1021:1027	deep tissue cancer imaging	1002:1027	deep tissue cancer imaging	1002:1027	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	3	6	theme	hyaluronic	548:557	arg1	acid					559:562	hyaluronic acid	548:562	hyaluronic acid	548:562	Moreover, PCN-58-Ps is capped with hyaluronic acid through coordination to obtain cancer cell-specific targeting properties.					
33617221	5	7	theme	two-photon	983:992	arg1	PDT					994:996	near-infrared light-activated two-photon PDT	953:996	near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications	953:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	0	8	with	Therapy	76:82	arg1	Excitation					103:112	Near-Infrared Excitation	89:112	Near-Infrared Excitation	89:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	4	9	theme	ROS	810:812	arg1	activity					721:728	considerable two-photon activity	697:728	considerable two-photon activity (upon laser excitation at a wavelength of 910 nm)	697:778	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	9	theme	ROS	810:812	arg1	ability					840:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	9	theme	ROS	810:812	arg1	result					643:648	a result	641:648	a result	641:648	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	10	theme	two-photon	710:719	arg1	activity					721:728	considerable two-photon activity	697:728	considerable two-photon activity (upon laser excitation at a wavelength of 910 nm)	697:778	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	10	theme	two-photon	710:719	arg1	ability					840:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	10	theme	two-photon	710:719	arg1	result					643:648	a result	641:648	a result	641:648	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	1	11	theme	tissue	190:195	arg1	imaging					216:222	deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability	185:294	imaging	216:222	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	2	12	theme	metal-organic	455:467	arg1	framework					469:477	a two-photon-active metal-organic framework	435:477	a two-photon-active metal-organic framework	435:477	Therefore, we use a rational strategy to fabricate a two-photon-active metal-organic framework via a click reaction (PCN-58-Ps).					
33617221	1	13	theme	focal	197:201	arg1	imaging					216:222	deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability	185:294	imaging	216:222	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	4	14	theme	optimized	655:663	arg1	PCN-58-Ps-HA					675:686	the optimized composite PCN-58-Ps-HA	651:686	the optimized composite PCN-58-Ps-HA	651:686	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	1	15	theme	fluorescence	203:214	arg1	imaging					216:222	deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability	185:294	imaging	216:222	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	0	16	theme	Click	0:4	arg1	Modification					6:17	Click Modification	0:17	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation	0:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	5	17	theme	clinical	1081:1088	arg1	applications					1090:1101	future clinical applications	1074:1101	future clinical applications	1074:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	5	18	theme	near-infrared	953:965	arg1	PDT					994:996	near-infrared light-activated two-photon PDT	953:996	near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications	953:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	4	19	theme	considerable	697:708	arg1	activity					721:728	considerable two-photon activity	697:728	considerable two-photon activity (upon laser excitation at a wavelength of 910 nm)	697:778	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	19	theme	considerable	697:708	arg1	ability					840:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	19	theme	considerable	697:708	arg1	result					643:648	a result	641:648	a result	641:648	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	2	20	theme	two-photon-active	437:453	arg1	framework					469:477	a two-photon-active metal-organic framework	435:477	a two-photon-active metal-organic framework	435:477	Therefore, we use a rational strategy to fabricate a two-photon-active metal-organic framework via a click reaction (PCN-58-Ps).					
33617221	1	21	theme	great	302:306	arg1	interest					308:315	great interest	302:315	great interest	302:315	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	5	22	theme	great	1054:1058	arg1	potential					1060:1068	great potential	1054:1068	great potential for future clinical applications	1054:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	5	23	theme	light-activated	967:981	arg1	PDT					994:996	near-infrared light-activated two-photon PDT	953:996	near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications	953:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	4	24	theme	generation	829:838	arg1	activity					721:728	considerable two-photon activity	697:728	considerable two-photon activity (upon laser excitation at a wavelength of 910 nm)	697:778	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	24	theme	generation	829:838	arg1	ability					840:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	24	theme	generation	829:838	arg1	result					643:648	a result	641:648	a result	641:648	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	5	25	theme	deep	1002:1005	arg1	imaging					1021:1027	deep tissue cancer imaging	1002:1027	deep tissue cancer imaging	1002:1027	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	4	26	theme	nm	776:777	arg1	excitation					742:751	laser excitation	736:751	laser excitation	736:751	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	5	27	theme	critical	888:895	arg1	factors					897:903	these two critical factors	878:903	these two critical factors	878:903	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	0	28	theme	Framework	38:46	arg1	Modification					6:17	Click Modification	0:17	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation	0:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	4	29	dep	nm	776:777	arg1	a					756:756	a	756:756	a	756:756	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	29	dep	nm	776:777	arg1	wavelength					758:767	wavelength	758:767	wavelength	758:767	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	30	theme	composite	665:673	arg1	PCN-58-Ps-HA					675:686	the optimized composite PCN-58-Ps-HA	651:686	the optimized composite PCN-58-Ps-HA	651:686	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	3	31	theme	targeting	616:624	arg1	properties					626:635	cancer cell-specific targeting properties	595:635	cancer cell-specific targeting properties	595:635	Moreover, PCN-58-Ps is capped with hyaluronic acid through coordination to obtain cancer cell-specific targeting properties.					
33617221	1	32	theme	excellent	237:245	arg1	generation					277:286	excellent reactive oxygen species (ROS) generation	237:286	excellent reactive oxygen species (ROS) generation	237:286	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	0	33	theme	Metal-Organic	24:36	arg1	Framework					38:46	a Metal-Organic Framework	22:46	a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation	22:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	2	34	theme	click	485:489	arg1	PCN-58-Ps					501:509	PCN-58-Ps	501:509	PCN-58-Ps	501:509	Therefore, we use a rational strategy to fabricate a two-photon-active metal-organic framework via a click reaction (PCN-58-Ps).					
33617221	2	34	theme	click	485:489	arg1	reaction					491:498	a click reaction	483:498	a click reaction (PCN-58-Ps)	483:510	Therefore, we use a rational strategy to fabricate a two-photon-active metal-organic framework via a click reaction (PCN-58-Ps).					
33617221	1	35	dep	imaging	216:222	arg1	ability					288:294	ability	288:294	ability	288:294	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	1	35	dep	imaging	216:222	arg1	capacity					224:231	capacity	224:231	capacity	224:231	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	1	36	theme	reactive	247:254	arg1	ROS					272:274	ROS	272:274	ROS	272:274	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	1	36	theme	reactive	247:254	arg1	species					263:269	reactive oxygen species	247:269	excellent reactive oxygen species (ROS) generation	237:286	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	0	37	theme	Two-Photon	52:61	arg1	Therapy					76:82	Two-Photon Photodynamic Therapy	52:82	Two-Photon Photodynamic Therapy with Near-Infrared Excitation	52:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	3	38	theme	cancer	595:600	arg1	properties					626:635	cancer cell-specific targeting properties	595:635	cancer cell-specific targeting properties	595:635	Moreover, PCN-58-Ps is capped with hyaluronic acid through coordination to obtain cancer cell-specific targeting properties.					
33617221	5	39	theme	PCN-58-Ps-HA	916:927	arg1	framework					929:937	the PCN-58-Ps-HA framework	912:937	the PCN-58-Ps-HA framework	912:937	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	1	40	theme	high-priority	332:344	arg1	demand					346:351	the high-priority demand	328:351	the high-priority demand of photodynamic therapy (PDT)	328:381	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	3	41	theme	cell-specific	602:614	arg1	properties					626:635	cancer cell-specific targeting properties	595:635	cancer cell-specific targeting properties	595:635	Moreover, PCN-58-Ps is capped with hyaluronic acid through coordination to obtain cancer cell-specific targeting properties.					
33617221	1	42	theme	effective	135:143	arg1	strategies					145:154	effective strategies	135:154	effective strategies	135:154	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	5	43	theme	cancer	1014:1019	arg1	imaging					1021:1027	deep tissue cancer imaging	1002:1027	deep tissue cancer imaging	1002:1027	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	1	44	theme	strategies	145:154	arg1	exploitation					119:130	The exploitation	115:130	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability	115:294	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	5	45	contain	has	1050:1052	arg2	potential					1060:1068	great potential	1054:1068	great potential for future clinical applications	1054:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	5	45	contain	has	1050:1052	arg1	treatment					1033:1041	treatment	1033:1041	treatment	1033:1041	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	5	45	contain	has	1050:1052	arg1	imaging					1021:1027	deep tissue cancer imaging	1002:1027	deep tissue cancer imaging	1002:1027	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	1	46	theme	oxygen	256:261	arg1	ROS					272:274	ROS	272:274	ROS	272:274	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	1	46	theme	oxygen	256:261	arg1	species					263:269	reactive oxygen species	247:269	excellent reactive oxygen species (ROS) generation	237:286	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	4	47	theme	light-triggered	794:808	arg1	ROS					810:812	light-triggered ROS	794:812	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	5	48	theme	future	1074:1079	arg1	applications					1090:1101	future clinical applications	1074:1101	future clinical applications	1074:1101	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
33617221	4	49	theme	excellent	784:792	arg1	activity					721:728	considerable two-photon activity	697:728	considerable two-photon activity (upon laser excitation at a wavelength of 910 nm)	697:778	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	49	theme	excellent	784:792	arg1	ability					840:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	excellent light-triggered ROS (1O2 and O2•-) generation ability	784:846	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	49	theme	excellent	784:792	arg1	result					643:648	a result	641:648	a result	641:648	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	1	50	theme	species	263:269	arg1	generation					277:286	excellent reactive oxygen species (ROS) generation	237:286	excellent reactive oxygen species (ROS) generation	237:286	The exploitation of effective strategies to develop materials bearing deep tissue focal fluorescence imaging capacity and excellent reactive oxygen species (ROS) generation ability is of great interest to address the high-priority demand of photodynamic therapy (PDT).					
33617221	2	51	theme	rational	404:411	arg1	strategy					413:420	a rational strategy	402:420	a rational strategy to fabricate a two-photon-active metal-organic framework via a click reaction (PCN-58-Ps)	402:510	Therefore, we use a rational strategy to fabricate a two-photon-active metal-organic framework via a click reaction (PCN-58-Ps).					
33617221	4	52	dep	ROS	810:812	arg1	O2•-					823:826	O2•-	823:826	O2•-	823:826	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	4	52	dep	ROS	810:812	arg1	1O2					815:817	1O2	815:817	1O2	815:817	As a result, the optimized composite PCN-58-Ps-HA exhibits considerable two-photon activity (upon laser excitation at a wavelength of 910 nm) and excellent light-triggered ROS (1O2 and O2•-) generation ability.					
33617221	0	53	theme	Photodynamic	63:74	arg1	Therapy					76:82	Two-Photon Photodynamic Therapy	52:82	Two-Photon Photodynamic Therapy with Near-Infrared Excitation	52:112	Click Modification of a Metal-Organic Framework for Two-Photon Photodynamic Therapy with Near-Infrared Excitation.					
33617221	5	54	theme	factors	897:903	arg1	interplay					865:873	the interplay	861:873	the interplay of these two critical factors within the PCN-58-Ps-HA framework	861:937	In summary, the interplay of these two critical factors within the PCN-58-Ps-HA framework gives rise to near-infrared light-activated two-photon PDT for deep tissue cancer imaging and treatment, which has great potential for future clinical applications.					
32574742	0	0	theme	composite	89:97	arg1	mats					107:110	PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats	36:110	PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats	36:110	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	7	1	theme	culture	1176:1182	arg1	studies					1184:1190	The in vitro cell culture studies	1158:1190	The in vitro cell culture studies	1158:1190	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	8	2	theme	tissue	1503:1508	arg1	engineering					1510:1520	skin tissue engineering	1498:1520	skin tissue engineering	1498:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	5	3	theme	water	873:877	arg1	rate					890:893	water absorption rate	873:893	water absorption rate	873:893	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	1	4	theme	amino-reserved	255:268	arg1	CS-g-PCL					270:277	amino-reserved CS-g-PCL	255:277	amino-reserved CS-g-PCL	255:277	In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method.					
32574742	7	5	dep	higher	1234:1239	arg1	in					1241:1242	in	1241:1242	in	1241:1242	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	0	6	dep	microstructure	117:130	arg1	assessment					163:172	assessment	163:172	assessment	163:172	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	8	7	theme	excellent	1413:1421	arg1	performance					1423:1433	excellent performance	1413:1433	excellent performance	1413:1433	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	0	8	theme	mats	107:110	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	0	8	theme	mats	107:110	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	8	9	theme	wound	1480:1484	arg1	dressing					1486:1493	wound dressing	1480:1493	wound dressing in skin tissue engineering	1480:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	8	9	theme	wound	1480:1484	arg1	mats					1403:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	3	10	theme	smooth	522:527	arg1	nanofibers					553:562	the smooth defect-free and uniform nanofibers	518:562	the smooth defect-free and uniform nanofibers	518:562	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	4	11	theme	mechanical	727:736	arg1	properties					738:747	The mechanical properties	723:747	The mechanical properties of composite mats	723:765	The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA.					
32574742	0	12	theme	fibrous	99:105	arg1	mats					107:110	PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats	36:110	PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats	36:110	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	8	13	theme	skin	1498:1501	arg1	engineering					1510:1520	skin tissue engineering	1498:1520	skin tissue engineering	1498:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	6	14	theme	PLLA	1152:1155	arg1	defects					1141:1147	the nature defects	1130:1147	the nature defects of PLLA	1130:1155	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	3	15	theme	defect-free	529:539	arg1	nanofibers					553:562	the smooth defect-free and uniform nanofibers	518:562	the smooth defect-free and uniform nanofibers	518:562	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	5	16	theme	transmission	908:919	arg1	rate					921:924	water-vapor transmission rate	896:924	water-vapor transmission rate	896:924	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	3	17	theme	CS-g-PCL	713:720	arg1	content					702:708	the increasing content	687:708	the increasing content of CS-g-PCL	687:720	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	2	18	theme	compositions	415:426	arg1	series					395:400	a series	393:400	a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%)	393:476	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	7	19	theme	in	1162:1163	arg1	studies					1184:1190	The in vitro cell culture studies	1158:1190	The in vitro cell culture studies	1158:1190	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	3	20	theme	increasing	691:700	arg1	content					702:708	the increasing content	687:708	the increasing content of CS-g-PCL	687:720	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	5	21	dep	in	930:931	arg1	vitro					933:937	vitro	933:937	vitro	933:937	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	4	22	theme	mats	762:765	arg1	properties					738:747	The mechanical properties	723:747	The mechanical properties of composite mats	723:765	The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA.					
32574742	6	23	theme	CS-g-PCL	1049:1056	arg1	addition					1037:1044	The addition	1033:1044	The addition of CS-g-PCL	1033:1056	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	2	24	theme	different	405:413	arg1	compositions					415:426	different compositions	405:426	different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%)	405:476	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	6	25	from	mats	1093:1096	arg1	properties					1108:1117	better properties	1101:1117	better properties	1101:1117	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	5	26	theme	absorption	879:888	arg1	rate					890:893	water absorption rate	873:893	water absorption rate	873:893	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	7	27	dep	in	1241:1242	arg1	vitro					1244:1248	vitro	1244:1248	vitro	1244:1248	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	5	28	theme	%	978:978	arg1	behavior					951:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	28	theme	%	978:978	arg1	%					983:983	129°-19°, 109%-482%	965:983	%	983:983	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	29	theme	rate	921:924	arg1	behavior					951:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	29	theme	rate	921:924	arg1	%					983:983	129°-19°, 109%-482%	965:983	%	983:983	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	30	theme	water	852:856	arg1	angle					866:870	water contact angle	852:870	water contact angle	852:870	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	4	31	theme	neat	837:840	arg1	PLLA					842:845	neat PLLA	837:845	neat PLLA	837:845	The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA.					
32574742	2	32	theme	dichloromethane/ethanol	324:346	arg1	solvent					362:368	dichloromethane/ethanol (CH2Cl2/EtOH) solvent	324:368	dichloromethane/ethanol (CH2Cl2/EtOH) solvent	324:368	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	6	33	theme	nature	1134:1139	arg1	defects					1141:1147	the nature defects	1130:1147	the nature defects of PLLA	1130:1155	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	5	34	theme	contact	858:864	arg1	angle					866:870	water contact angle	852:870	water contact angle	852:870	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	1	35	theme	one-step	298:305	arg1	method					307:312	one-step method	298:312	one-step method	298:312	In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method.					
32574742	5	36	theme	in	930:931	arg1	degradation					939:949	in vitro degradation	930:949	in vitro degradation	930:949	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	6	37	dep	imparted	1058:1065	arg1	improving					1120:1128	improving	1120:1128	improving the nature defects of PLLA	1120:1155	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	5	38	theme	degradation	939:949	arg1	behavior					951:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	38	theme	degradation	939:949	arg1	%					983:983	129°-19°, 109%-482%	965:983	%	983:983	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	8	39	from	dressing	1486:1493	arg1	engineering					1510:1520	skin tissue engineering	1498:1520	skin tissue engineering	1498:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	3	40	theme	fiber	631:635	arg1	diameter					637:644	the fiber diameter	627:644	the fiber diameter	627:644	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	3	41	theme	uniform	545:551	arg1	nanofibers					553:562	the smooth defect-free and uniform nanofibers	518:562	the smooth defect-free and uniform nanofibers	518:562	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	2	42	theme	mats	445:448	arg1	compositions					415:426	different compositions	405:426	different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%)	405:476	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	2	43	theme	wt/wt	470:474	arg1	%					475:475	8/2, 6/4, 4/6, 2/8 wt/wt%	451:475	8/2, 6/4, 4/6, 2/8 wt/wt%	451:475	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	2	43	theme	wt/wt	470:474	arg1	mats					445:448	PLLA/CS-g-PCL mats	431:448	PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%)	431:476	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	5	44	theme	-482	979:982	arg1	behavior					951:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	44	theme	-482	979:982	arg1	%					983:983	129°-19°, 109%-482%	965:983	%	983:983	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	1	45	theme	chitosan	218:225	arg1	electrospinning					199:213	the electrospinning	195:213	the electrospinning of chitosan in common organic solvents	195:252	In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method.					
32574742	3	46	theme	PLLA/CS-g-PCL	586:598	arg1	2/8					600:602	PLLA/CS-g-PCL 2/8	586:602	PLLA/CS-g-PCL 2/8	586:602	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	7	47	theme	cell	1292:1295	arg1	attachment					1297:1306	cell attachment	1292:1306	cell attachment	1292:1306	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	2	48	theme	PLLA/CS-g-PCL	431:443	arg1	%					475:475	8/2, 6/4, 4/6, 2/8 wt/wt%	451:475	8/2, 6/4, 4/6, 2/8 wt/wt%	451:475	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	2	48	theme	PLLA/CS-g-PCL	431:443	arg1	mats					445:448	PLLA/CS-g-PCL mats	431:448	PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%)	431:476	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	0	49	theme	PLLA/chitosan-graft-poly	36:59	arg1	mats					107:110	PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats	36:110	PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats	36:110	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	8	50	with	mats	1403:1406	arg1	performance					1423:1433	excellent performance	1413:1433	excellent performance	1413:1433	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	7	51	theme	PLLA	1354:1357	arg1	mats					1359:1362	PLLA mats	1354:1362	PLLA mats	1354:1362	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	3	52	dep	461 nm	675:680	arg1	to					672:673	to	672:673	to	672:673	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	6	53	theme	electrospun	1081:1091	arg1	mats					1093:1096	PLLA/CS-g-PCL electrospun mats	1067:1096	PLLA/CS-g-PCL electrospun mats on better properties	1067:1117	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	5	54	theme	rate	890:893	arg1	behavior					951:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	54	theme	rate	890:893	arg1	%					983:983	129°-19°, 109%-482%	965:983	%	983:983	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	7	55	dep	in	1162:1163	arg1	vitro					1165:1169	vitro	1165:1169	vitro	1165:1169	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	8	56	theme	electrospun	1391:1401	arg1	dressing					1486:1493	wound dressing	1480:1493	wound dressing in skin tissue engineering	1480:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	8	56	theme	electrospun	1391:1401	arg1	mats					1403:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	1	57	from	electrospinning	199:213	arg1	solvents					245:252	common organic solvents	230:252	common organic solvents	230:252	In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method.					
32574742	6	58	theme	better	1101:1106	arg1	properties					1108:1117	better properties	1101:1117	better properties	1101:1117	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	3	59	theme	pure	608:611	arg1	CS-g-PCL					613:620	pure CS-g-PCL	608:620	pure CS-g-PCL	608:620	The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL.					
32574742	8	60	theme	6/4	1387:1389	arg1	dressing					1486:1493	wound dressing	1480:1493	wound dressing in skin tissue engineering	1480:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	8	60	theme	6/4	1387:1389	arg1	mats					1403:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	5	61	theme	water-vapor	896:906	arg1	rate					921:924	water-vapor transmission rate	896:924	water-vapor transmission rate	896:924	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	1	62	theme	common	230:235	arg1	solvents					245:252	common organic solvents	230:252	common organic solvents	230:252	In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method.					
32574742	8	63	theme	potential	1455:1463	arg1	application					1465:1475	the potential application	1451:1475	the potential application	1451:1475	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	4	64	theme	composite	752:760	arg1	mats					762:765	composite mats	752:765	composite mats	752:765	The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA.					
32574742	2	65	theme	CH2Cl2/EtOH	349:359	arg1	solvent					362:368	dichloromethane/ethanol (CH2Cl2/EtOH) solvent	324:368	dichloromethane/ethanol (CH2Cl2/EtOH) solvent	324:368	PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning.					
32574742	1	66	theme	organic	237:243	arg1	solvents					245:252	common organic solvents	230:252	common organic solvents	230:252	In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method.					
32574742	8	67	theme	PLLA/CS-g-PCL	1373:1385	arg1	dressing					1486:1493	wound dressing	1480:1493	wound dressing in skin tissue engineering	1480:1520	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	8	67	theme	PLLA/CS-g-PCL	1373:1385	arg1	mats					1403:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	PLLA/CS-g-PCL 6/4 electrospun mats	1373:1406	Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.					
32574742	4	68	theme	CS-g-PCL	798:805	arg1	content					807:813	CS-g-PCL content	798:813	CS-g-PCL content	798:813	The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA.					
32574742	7	69	theme	better	1273:1278	arg1	ability					1280:1286	a better ability	1271:1286	a better ability for cell attachment, spreading, and proliferation	1271:1336	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	5	70	theme	angle	866:870	arg1	behavior					951:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior	848:958	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	5	70	theme	angle	866:870	arg1	%					983:983	129°-19°, 109%-482%	965:983	%	983:983	The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°-19°, 109%-482%, 1945-2517 g m-2 day-1 and 4-14%, respectively.					
32574742	0	71	dep	Preparation	0:10	arg1	performance					133:143	performance	133:143	performance	133:143	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	0	71	dep	Preparation	0:10	arg1	microstructure					117:130	The microstructure	113:130	The microstructure	113:130	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	0	71	dep	Preparation	0:10	arg1	proliferation					149:161	proliferation	149:161	proliferation	149:161	Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment.					
32574742	7	72	theme	higher	1234:1239	arg1	biocompatibility					1250:1265	a higher in vitro biocompatibility	1232:1265	a higher in vitro biocompatibility	1232:1265	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	6	73	theme	PLLA/CS-g-PCL	1067:1079	arg1	mats					1093:1096	PLLA/CS-g-PCL electrospun mats	1067:1096	PLLA/CS-g-PCL electrospun mats on better properties	1067:1117	The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA.					
32574742	7	74	theme	PLLA/CS-g-PCL	1204:1216	arg1	6/4					1218:1220	PLLA/CS-g-PCL 6/4	1204:1220	PLLA/CS-g-PCL 6/4	1204:1220	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
32574742	7	75	theme	cell	1171:1174	arg1	studies					1184:1190	The in vitro cell culture studies	1158:1190	The in vitro cell culture studies	1158:1190	The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats.					
33436038	5	0	theme	electron	704:711	arg1	microscope					713:722	a scanning electron microscope	693:722	a scanning electron microscope	693:722	The characteristics of NGF-CS/HA-coating implants were determined using a scanning electron microscope.					
33436038	6	1	theme	X-ray	891:895	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	2	theme	dogs	1828:1831	arg1	mandible					1809:1816	the mandible	1805:1816	the mandible of Beagle dogs	1805:1831	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	6	3	theme	blue	972:975	arg1	staining					977:984	toluidine blue staining	962:984	toluidine blue staining	962:984	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	4	theme	NGF-CS/HA-coating	1654:1670	arg1	implants					1682:1689	NGF-CS/HA-coating composite implants	1654:1689	NGF-CS/HA-coating composite implants	1654:1689	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	8	5	theme	NGF-CS/HA-coating	1236:1252	arg1	implants					1254:1261	NGF-CS/HA-coating implants	1236:1261	NGF-CS/HA-coating implants	1236:1261	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	10	6	from	effects	1717:1723	arg1	osseointegration					1738:1753	the early osseointegration	1728:1753	the early osseointegration	1728:1753	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	10	6	from	effects	1717:1723	arg1	regeneration					1765:1776	nerve regeneration	1759:1776	nerve regeneration	1759:1776	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	6	7	theme	fluorescence	939:950	arg1	staining					952:959	double fluorescence staining	932:959	double fluorescence staining	932:959	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	8	theme	significant	1695:1705	arg1	effects					1717:1723	significant induction effects	1695:1723	significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues	1695:1800	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	2	9	theme	bone	325:328	arg1	healing					330:336	bone healing	325:336	bone healing	325:336	Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing.					
33436038	1	10	theme	function	233:240	arg1	Osseointegration					186:201	BACKGROUND Osseointegration	175:201	BACKGROUND Osseointegration	175:201	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	1	10	theme	function	233:240	arg1	premise					210:216	the premise	206:216	the premise of the chewing function of dental implant	206:258	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	6	11	theme	RT-qPCR	1032:1038	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	12	theme	implants	1682:1689	arg1	Implantation					1638:1649	CONCLUSION Implantation	1627:1649	CONCLUSION Implantation of NGF-CS/HA-coating composite implants	1627:1689	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	0	13	theme	Beagle	162:167	arg1	dogs					169:172	Beagle dogs	162:172	Beagle dogs	162:172	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	3	14	from	influence	352:360	arg1	innervations					468:479	innervations	468:479	innervations	468:479	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	3	14	from	influence	352:360	arg1	osseointegration					447:462	osseointegration	447:462	osseointegration	447:462	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	9	15	theme	osteogenesis	1520:1531	arg1	differentiation					1533:1547	osteogenesis differentiation	1520:1547	osteogenesis differentiation	1520:1547	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	7	16	dep	components	1170:1179	arg1	HA					1189:1190	HA	1189:1190	HA	1189:1190	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	7	16	dep	components	1170:1179	arg1	Ti					1182:1183	Ti	1182:1183	Ti	1182:1183	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	7	16	dep	components	1170:1179	arg1	components					1170:1179	the main components	1161:1179	the main components (Ti and HA)	1161:1191	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	8	17	from	output	1337:1342	arg1	mandible					1378:1385	the mandible	1374:1385	the mandible of Beagle dogs	1374:1400	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	8	18	theme	tissues	1308:1314	arg1	morphology					1289:1298	the morphology	1285:1298	the morphology of bone tissues	1285:1314	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	10	19	theme	composite	1672:1680	arg1	implants					1682:1689	NGF-CS/HA-coating composite implants	1654:1689	NGF-CS/HA-coating composite implants	1654:1689	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	7	20	theme	porous	1133:1138	arg1	structures					1145:1154	porous mesh structures	1133:1154	porous mesh structures	1133:1154	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	2	21	theme	Nerve	261:265	arg1	factor					274:279	Nerve growth factor	261:279	Nerve growth factor (NGF)	261:285	Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing.					
33436038	2	21	theme	Nerve	261:265	arg1	NGF					282:284	NGF	282:284	NGF	282:284	Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing.					
33436038	9	22	theme	neurogenic	1554:1563	arg1	genes					1589:1593	neurogenic differentiation-related genes	1554:1593	neurogenic differentiation-related genes	1554:1593	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	6	23	theme	neural	991:996	arg1	tracer					998:1003	DiI neural tracer	987:1003	DiI neural tracer	987:1003	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	8	24	from	BIC	1350:1352	arg1	mandible					1378:1385	the mandible	1374:1385	the mandible of Beagle dogs	1374:1400	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	9	25	theme	genes	1589:1593	arg1	levels					1505:1510	the levels	1501:1510	the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes	1501:1593	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	0	26	theme	nerve	116:120	arg1	regeneration					122:133	nerve regeneration	116:133	nerve regeneration	116:133	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	8	27	from	fiber	1365:1369	arg1	mandible					1378:1385	the mandible	1374:1385	the mandible of Beagle dogs	1374:1400	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	3	28	theme	NGF-chondroitin	365:379	arg1	CS					390:391	CS	390:391	CS	390:391	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	3	28	theme	NGF-chondroitin	365:379	arg1	sulfate					381:387	NGF-chondroitin sulfate	365:387	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	8	29	theme	dogs	1397:1400	arg1	mandible					1378:1385	the mandible	1374:1385	the mandible of Beagle dogs	1374:1400	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	5	30	theme	NGF-CS/HA-coating	644:660	arg1	implants					662:669	NGF-CS/HA-coating implants	644:669	NGF-CS/HA-coating implants	644:669	The characteristics of NGF-CS/HA-coating implants were determined using a scanning electron microscope.					
33436038	9	31	theme	dogs	1621:1624	arg1	mandible					1602:1609	the mandible	1598:1609	the mandible of Beagle dogs	1598:1624	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	1	32	theme	BACKGROUND	175:184	arg1	Osseointegration					186:201	BACKGROUND Osseointegration	175:201	BACKGROUND Osseointegration	175:201	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	1	32	theme	BACKGROUND	175:184	arg1	premise					210:216	the premise	206:216	the premise of the chewing function of dental implant	206:258	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	0	33	theme	tissues	151:157	arg1	osseointegration					95:110	early osseointegration	89:110	early osseointegration	89:110	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	0	33	theme	tissues	151:157	arg1	regeneration					122:133	nerve regeneration	116:133	nerve regeneration	116:133	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	3	34	theme	composite	422:430	arg1	implant					432:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	0	35	theme	Nerve	0:4	arg1	factor-chondroitin					13:30	Nerve growth factor-chondroitin	0:30	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant	0:79	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	6	36	theme	double	932:937	arg1	staining					952:959	double fluorescence staining	932:959	double fluorescence staining	932:959	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	6	37	from	innervation	833:843	arg1	tissues					861:867	peri-implant tissues	848:867	peri-implant tissues	848:867	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	38	theme	peri-implant	1781:1792	arg1	tissues					1794:1800	peri-implant tissues	1781:1800	peri-implant tissues	1781:1800	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	0	39	theme	composite	63:71	arg1	implant					73:79	composite implant	63:79	composite implant	63:79	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	6	40	theme	force	925:929	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	41	contain	has	1691:1693	arg2	effects					1717:1723	significant induction effects	1695:1723	significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues	1695:1800	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	10	41	contain	has	1691:1693	arg1	Implantation					1638:1649	CONCLUSION Implantation	1627:1649	CONCLUSION Implantation of NGF-CS/HA-coating composite implants	1627:1689	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	10	42	theme	Beagle	1821:1826	arg1	dogs					1828:1831	Beagle dogs	1821:1831	Beagle dogs	1821:1831	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	6	43	theme	maximal	908:914	arg1	force					925:929	maximal pull-out force	908:929	maximal pull-out force	908:929	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	6	44	theme	Micro-CT	898:905	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	6	45	from	osseointegration	812:827	arg1	tissues					861:867	peri-implant tissues	848:867	peri-implant tissues	848:867	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	46	theme	CONCLUSION	1627:1636	arg1	Implantation					1638:1649	CONCLUSION Implantation	1627:1649	CONCLUSION Implantation of NGF-CS/HA-coating composite implants	1627:1689	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	6	47	theme	dogs	796:799	arg1	mandible					777:784	the mandible	773:784	the mandible of Beagle dogs	773:799	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	6	48	theme	DiI	987:989	arg1	tracer					998:1003	DiI neural tracer	987:1003	DiI neural tracer	987:1003	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	5	49	theme	scanning	695:702	arg1	microscope					713:722	a scanning electron microscope	693:722	a scanning electron microscope	693:722	The characteristics of NGF-CS/HA-coating implants were determined using a scanning electron microscope.					
33436038	9	50	theme	implants	1466:1473	arg1	implantation					1432:1443	the implantation	1428:1443	the implantation of NGF-CS/HA-coating implants	1428:1473	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	6	51	theme	staining	977:984	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	4	52	theme	biomimetic	602:611	arg1	method					613:618	the modified biomimetic method	589:618	the modified biomimetic method	589:618	MATERIALS AND METHODS NGF-CS/HA-coating composite implants were prepared using the modified biomimetic method.					
33436038	1	53	theme	dental	245:250	arg1	implant					252:258	dental implant	245:258	dental implant	245:258	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	6	54	theme	toluidine	962:970	arg1	staining					977:984	toluidine blue staining	962:984	toluidine blue staining	962:984	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	2	55	theme	neurotrophic	293:304	arg1	factor					306:311	a neurotrophic factor	291:311	a neurotrophic factor	291:311	Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing.					
33436038	6	56	theme	staining	952:959	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	57	theme	induction	1707:1715	arg1	effects					1717:1723	significant induction effects	1695:1723	significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues	1695:1800	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	7	58	theme	main	1165:1168	arg1	HA					1189:1190	HA	1189:1190	HA	1189:1190	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	7	58	theme	main	1165:1168	arg1	Ti					1182:1183	Ti	1182:1183	Ti	1182:1183	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	7	58	theme	main	1165:1168	arg1	components					1170:1179	the main components	1161:1179	the main components (Ti and HA)	1161:1191	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	6	59	theme	peri-implant	848:859	arg1	tissues					861:867	peri-implant tissues	848:867	peri-implant tissues	848:867	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	1	60	theme	chewing	225:231	arg1	function					233:240	the chewing function	221:240	the chewing function of dental implant	221:258	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	8	61	theme	implants	1254:1261	arg1	implantation					1220:1231	implantation	1220:1231	implantation of NGF-CS/HA-coating implants	1220:1261	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	8	62	theme	maximum	1329:1335	arg1	output					1337:1342	maximum output	1329:1342	maximum output	1329:1342	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	10	63	theme	early	1732:1736	arg1	osseointegration					1738:1753	the early osseointegration	1728:1753	the early osseointegration	1728:1753	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	7	64	theme	mesh	1140:1143	arg1	structures					1145:1154	porous mesh structures	1133:1154	porous mesh structures	1133:1154	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	9	65	theme	NGF	1515:1517	arg1	levels					1505:1510	the levels	1501:1510	the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes	1501:1593	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	9	66	theme	differentiation	1533:1547	arg1	levels					1505:1510	the levels	1501:1510	the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes	1501:1593	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	6	67	theme	immunohistochemistry	1006:1025	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	2	68	theme	growth	267:272	arg1	factor					274:279	Nerve growth factor	261:279	Nerve growth factor (NGF)	261:285	Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing.					
33436038	2	68	theme	growth	267:272	arg1	NGF					282:284	NGF	282:284	NGF	282:284	Nerve growth factor (NGF), as a neurotrophic factor, can induce bone healing.					
33436038	8	69	theme	bone	1303:1306	arg1	tissues					1308:1314	bone tissues	1303:1314	bone tissues	1303:1314	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	6	70	theme	tracer	998:1003	arg1	assays					1040:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays	891:1045	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	9	71	theme	differentiation-related	1565:1587	arg1	genes					1589:1593	neurogenic differentiation-related genes	1554:1593	neurogenic differentiation-related genes	1554:1593	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	8	72	theme	nerve	1359:1363	arg1	fiber					1365:1369	nerve fiber	1359:1369	nerve fiber	1359:1369	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	7	73	theme	composite	1074:1082	arg1	implants					1084:1091	composite implants	1074:1091	composite implants	1074:1091	RESULTS NGF-CS/HA-coating composite implants were made successfully, which presented porous mesh structures with the main components (Ti and HA).					
33436038	0	74	theme	peri-implant	138:149	arg1	tissues					151:157	peri-implant tissues	138:157	peri-implant tissues	138:157	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	9	75	theme	Beagle	1614:1619	arg1	dogs					1621:1624	Beagle dogs	1614:1624	Beagle dogs	1614:1624	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33436038	3	76	theme	sulfate	381:387	arg1	implant					432:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	8	77	theme	Beagle	1390:1395	arg1	dogs					1397:1400	Beagle dogs	1390:1400	Beagle dogs	1390:1400	Besides, we revealed that implantation of NGF-CS/HA-coating implants significantly changed the morphology of bone tissues and elevated maximum output, MAR, BIC, and nerve fiber in the mandible of Beagle dogs.					
33436038	5	78	theme	implants	662:669	arg1	characteristics					625:639	The characteristics	621:639	The characteristics of NGF-CS/HA-coating implants	621:669	The characteristics of NGF-CS/HA-coating implants were determined using a scanning electron microscope.					
33436038	10	79	theme	nerve	1759:1763	arg1	regeneration					1765:1776	nerve regeneration	1759:1776	nerve regeneration	1759:1776	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	0	80	theme	growth	6:11	arg1	factor-chondroitin					13:30	Nerve growth factor-chondroitin	0:30	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant	0:79	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	3	81	theme	/hydroxyapatite	393:407	arg1	implant					432:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	3	82	theme	implant	432:438	arg1	clear					503:507	clear	503:507	clear	503:507	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	3	82	theme	implant	432:438	arg1	influence					352:360	the influence	348:360	the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations	348:479	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	4	83	theme	modified	593:600	arg1	method					613:618	the modified biomimetic method	589:618	the modified biomimetic method	589:618	MATERIALS AND METHODS NGF-CS/HA-coating composite implants were prepared using the modified biomimetic method.					
33436038	6	84	theme	NGF-CS/HA-coating	731:747	arg1	implants					749:756	NGF-CS/HA-coating implants	731:756	NGF-CS/HA-coating implants	731:756	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	10	85	theme	tissues	1794:1800	arg1	effects					1717:1723	significant induction effects	1695:1723	significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues	1695:1800	CONCLUSION Implantation of NGF-CS/HA-coating composite implants has significant induction effects on the early osseointegration and nerve regeneration of peri-implant tissues in the mandible of Beagle dogs.					
33436038	1	86	theme	implant	252:258	arg1	function					233:240	the chewing function	221:240	the chewing function of dental implant	221:258	BACKGROUND Osseointegration is the premise of the chewing function of dental implant.					
33436038	3	87	theme	-coating	413:420	arg1	implant					432:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	4	88	theme	composite	550:558	arg1	implants					560:567	composite implants	550:567	composite implants	550:567	MATERIALS AND METHODS NGF-CS/HA-coating composite implants were prepared using the modified biomimetic method.					
33436038	0	89	theme	early	89:93	arg1	osseointegration					95:110	early osseointegration	89:110	early osseointegration	89:110	Nerve growth factor-chondroitin sulfate/hydroxyapatite-coating composite implant induces early osseointegration and nerve regeneration of peri-implant tissues in Beagle dogs.					
33436038	6	90	theme	pull-out	916:923	arg1	force					925:929	maximal pull-out force	908:929	maximal pull-out force	908:929	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	3	91	theme	HA	410:411	arg1	implant					432:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant	365:438	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	6	92	theme	early	806:810	arg1	osseointegration					812:827	the early osseointegration	802:827	the early osseointegration	802:827	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	3	93	dep	osseointegration	447:462	arg1	the					443:445	the	443:445	the	443:445	However, the influence of NGF-chondroitin sulfate (CS)/hydroxyapatite (HA)-coating composite implant on the osseointegration and innervations is still not entirely clear.					
33436038	6	94	theme	Beagle	789:794	arg1	dogs					796:799	Beagle dogs	789:799	Beagle dogs	789:799	After NGF-CS/HA-coating implants were placed in the mandible of Beagle dogs, the early osseointegration and innervation in peri-implant tissues were assessed through X-ray, Micro-CT, maximal pull-out force, double fluorescence staining, toluidine blue staining, DiI neural tracer, immunohistochemistry, and RT-qPCR assays.					
33436038	9	95	theme	NGF-CS/HA-coating	1448:1464	arg1	implants					1466:1473	NGF-CS/HA-coating implants	1448:1473	NGF-CS/HA-coating implants	1448:1473	Moreover, we proved that the implantation of NGF-CS/HA-coating implants also markedly upregulated the levels of NGF, osteogenesis differentiation, and neurogenic differentiation-related genes in the mandible of Beagle dogs.					
33881045	4	0	theme	diabetic	662:669	arg1	wounds					671:676	diabetic wounds	662:676	diabetic wounds	662:676	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	1	1	theme	dangshen	176:183	arg1	TEP					192:194	TEP	192:194	TEP	192:194	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	1	1	theme	dangshen	176:183	arg1	pills					185:189	The Tibetan eighteen flavor dangshen pills	148:189	The Tibetan eighteen flavor dangshen pills (TEP)	148:195	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	6	2	theme	hydrogel	918:925	arg1	system					927:932	the hydrogel system	914:932	the hydrogel system	914:932	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	6	2	theme	hydrogel	918:925	arg1	cytotoxic					942:950	cytotoxic	942:950	cytotoxic	942:950	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	0	3	theme	medicine	93:100	arg1	release					62:68	sustained release	52:68	sustained release of traditional Tibetan medicine	52:100	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	7	4	theme	TEP-loaded	1042:1051	arg1	hydrogel					1053:1060	the TEP-loaded hydrogel	1038:1060	the TEP-loaded hydrogel	1038:1060	Moreover, when the TEP-loaded hydrogel was applied to diabetic wounds in rats, it reduced the inflammatory response and improved collagen deposition, which in turn promoted skin healing.					
33881045	0	5	theme	chronic	116:122	arg1	healing					139:145	chronic diabetic wound healing	116:145	chronic diabetic wound healing	116:145	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	1	6	from	diseases	296:303	arg1	system					328:333	the Tibetan medical system	308:333	the Tibetan medical system	308:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	5	7	contain	have	854:857	arg1	hydrogels					769:777	TEP-loaded hydrogels	758:777	TEP-loaded hydrogels	758:777	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	5	7	contain	have	854:857	arg2	properties					889:898	antibacterial and antioxidant properties	859:898	antibacterial and antioxidant properties	859:898	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	5	8	theme	antioxidant	877:887	arg1	properties					889:898	antibacterial and antioxidant properties	859:898	antibacterial and antioxidant properties	859:898	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	8	9	theme	chronic	1356:1362	arg1	wounds					1364:1369	chronic wounds	1356:1369	chronic wounds	1356:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	2	10	contain	have	341:344	arg2	effects					378:384	anti-inflammatory and analgesic effects	346:384	anti-inflammatory and analgesic effects	346:384	They have anti-inflammatory and analgesic effects, and healing properties.					
33881045	2	10	contain	have	341:344	arg1	They					336:339	They	336:339	They	336:339	They have anti-inflammatory and analgesic effects, and healing properties.					
33881045	2	10	contain	have	341:344	arg2	properties					399:408	healing properties	391:408	healing properties	391:408	They have anti-inflammatory and analgesic effects, and healing properties.					
33881045	8	11	theme	Tibetan	1317:1323	arg1	medicines					1325:1333	traditional Tibetan medicines	1305:1333	traditional Tibetan medicines for the treatment of chronic wounds	1305:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	2	12	theme	anti-inflammatory	346:362	arg1	effects					378:384	anti-inflammatory and analgesic effects	346:384	anti-inflammatory and analgesic effects	346:384	They have anti-inflammatory and analgesic effects, and healing properties.					
33881045	4	13	theme	TEP	568:570	arg1	powder					572:577	TEP powder	568:577	TEP powder	568:577	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	0	14	theme	wound	133:137	arg1	healing					139:145	chronic diabetic wound healing	116:145	chronic diabetic wound healing	116:145	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	8	15	theme	new	1266:1268	arg1	formulation					1270:1280	a new formulation	1264:1280	a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds	1264:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	8	15	theme	new	1266:1268	arg1	hydrogels					1247:1255	TEP-loaded hydrogels	1236:1255	TEP-loaded hydrogels	1236:1255	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	7	16	theme	diabetic	1077:1084	arg1	wounds					1086:1091	diabetic wounds	1077:1091	diabetic wounds in rats	1077:1099	Moreover, when the TEP-loaded hydrogel was applied to diabetic wounds in rats, it reduced the inflammatory response and improved collagen deposition, which in turn promoted skin healing.					
33881045	5	17	theme	active	824:829	arg1	ingredients					831:841	the active ingredients	820:841	the active ingredients of TEP	820:848	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	6	18	theme	HUVECs	1015:1020	arg1	proliferation					998:1010	the proliferation	994:1010	the proliferation of HUVECs	994:1020	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	2	19	theme	healing	391:397	arg1	properties					399:408	healing properties	391:408	healing properties	391:408	They have anti-inflammatory and analgesic effects, and healing properties.					
33881045	0	20	theme	diabetic	124:131	arg1	healing					139:145	chronic diabetic wound healing	116:145	chronic diabetic wound healing	116:145	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	7	21	theme	inflammatory	1117:1128	arg1	response					1130:1137	the inflammatory response	1113:1137	the inflammatory response	1113:1137	Moreover, when the TEP-loaded hydrogel was applied to diabetic wounds in rats, it reduced the inflammatory response and improved collagen deposition, which in turn promoted skin healing.					
33881045	4	22	theme	PVA/CS	635:640	arg1	hydrogels					643:651	polyvinyl alcohol/chitosan (PVA/CS) hydrogels	607:651	polyvinyl alcohol/chitosan (PVA/CS) hydrogels	607:651	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	7	23	theme	collagen	1152:1159	arg1	deposition					1161:1170	collagen deposition	1152:1170	collagen deposition	1152:1170	Moreover, when the TEP-loaded hydrogel was applied to diabetic wounds in rats, it reduced the inflammatory response and improved collagen deposition, which in turn promoted skin healing.					
33881045	7	24	theme	skin	1196:1199	arg1	healing					1201:1207	skin healing	1196:1207	skin healing	1196:1207	Moreover, when the TEP-loaded hydrogel was applied to diabetic wounds in rats, it reduced the inflammatory response and improved collagen deposition, which in turn promoted skin healing.					
33881045	5	25	theme	In	729:730	arg1	studies					738:744	In vitro studies	729:744	In vitro studies	729:744	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	4	26	theme	skin	540:543	arg1	treatment					545:553	skin treatment	540:553	skin treatment	540:553	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	1	27	from	treatment	278:286	arg1	system					328:333	the Tibetan medical system	308:333	the Tibetan medical system	308:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	5	28	theme	TEP	846:848	arg1	ingredients					831:841	the active ingredients	820:841	the active ingredients of TEP	820:848	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	8	29	theme	TEP-loaded	1236:1245	arg1	formulation					1270:1280	a new formulation	1264:1280	a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds	1264:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	8	29	theme	TEP-loaded	1236:1245	arg1	hydrogels					1247:1255	TEP-loaded hydrogels	1236:1255	TEP-loaded hydrogels	1236:1255	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	5	30	theme	antibacterial	859:871	arg1	properties					889:898	antibacterial and antioxidant properties	859:898	antibacterial and antioxidant properties	859:898	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	6	31	theme	L929	955:958	arg1	cells					960:964	L929 cells	955:964	L929 cells	955:964	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	8	32	theme	wounds	1364:1369	arg1	treatment					1343:1351	the treatment	1339:1351	the treatment of chronic wounds	1339:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	3	33	theme	strong	453:458	arg1	effects					465:471	strong side effects	453:471	strong side effects	453:471	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	0	34	theme	composite	27:35	arg1	hydrogels					37:45	composite hydrogels	27:45	composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing	27:145	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	6	35	from	cytotoxic	942:950	arg1	addition					904:911	addition	904:911	addition	904:911	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	3	36	contain	have	448:451	arg2	effects					465:471	strong side effects	453:471	strong side effects	453:471	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	3	36	contain	have	448:451	arg1	TEP					420:422	TEP	420:422	TEP	420:422	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	3	36	contain	have	448:451	arg2	bioavailability					481:495	low bioavailability	477:495	low bioavailability	477:495	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	4	37	theme	TEP	724:726	arg1	ingredients					709:719	the active ingredients	698:719	the active ingredients of TEP	698:726	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	3	38	theme	side	460:463	arg1	effects					465:471	strong side effects	453:471	strong side effects	453:471	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	0	39	theme	sustained	52:60	arg1	release					62:68	sustained release	52:68	sustained release of traditional Tibetan medicine	52:100	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	1	40	theme	skin	291:294	arg1	diseases					296:303	skin diseases	291:303	skin diseases in the Tibetan medical system	291:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	5	41	dep	In	729:730	arg1	vitro					732:736	vitro	732:736	vitro	732:736	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	1	42	used	used	266:269	arg2	medicines					236:244	18 traditional Tibetan medicines	213:244	18 traditional Tibetan medicines	213:244	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	3	43	theme	large	432:436	arg1	doses					438:442	large doses	432:442	large doses	432:442	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	1	44	theme	diseases	296:303	arg1	treatment					278:286	the treatment	274:286	the treatment of skin diseases in the Tibetan medical system	274:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	5	45	theme	TEP-loaded	758:767	arg1	hydrogels					769:777	TEP-loaded hydrogels	758:777	TEP-loaded hydrogels	758:777	In vitro studies showed that TEP-loaded hydrogels can effectively and continuously release the active ingredients of TEP and have antibacterial and antioxidant properties.					
33881045	0	46	with	hydrogels	37:45	arg1	release					62:68	sustained release	52:68	sustained release of traditional Tibetan medicine	52:100	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	1	47	theme	traditional	216:226	arg1	medicines					236:244	18 traditional Tibetan medicines	213:244	18 traditional Tibetan medicines	213:244	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	4	48	from	rate	525:528	arg1	treatment					545:553	skin treatment	540:553	skin treatment	540:553	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	4	49	theme	utilization	513:523	arg1	rate					525:528	the utilization rate	509:528	the utilization rate of TEP in skin treatment	509:553	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	3	50	theme	low	477:479	arg1	bioavailability					481:495	low bioavailability	477:495	low bioavailability	477:495	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	1	51	from	system	328:333	arg1	treatment					278:286	the treatment	274:286	the treatment of skin diseases in the Tibetan medical system	274:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	4	52	theme	TEP	533:535	arg1	rate					525:528	the utilization rate	509:528	the utilization rate of TEP in skin treatment	509:553	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	3	53	contain	contain	424:430	arg1	TEP					420:422	TEP	420:422	TEP	420:422	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	3	53	contain	contain	424:430	arg2	doses					438:442	large doses	432:442	large doses	432:442	However, TEP contain large doses and have strong side effects and low bioavailability.					
33881045	1	54	theme	Tibetan	312:318	arg1	system					328:333	the Tibetan medical system	308:333	the Tibetan medical system	308:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	6	55	from	addition	904:911	arg1	system					927:932	the hydrogel system	914:932	the hydrogel system	914:932	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	6	55	from	addition	904:911	arg1	cytotoxic					942:950	cytotoxic	942:950	cytotoxic	942:950	In addition, the hydrogel system was not cytotoxic to L929 cells, and significantly promoted the proliferation of HUVECs.					
33881045	4	56	theme	active	702:707	arg1	ingredients					709:719	the active ingredients	698:719	the active ingredients of TEP	698:726	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	1	57	theme	Tibetan	228:234	arg1	medicines					236:244	18 traditional Tibetan medicines	213:244	18 traditional Tibetan medicines	213:244	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	8	58	theme	medicines	1325:1333	arg1	application					1290:1300	the application	1286:1300	the application of traditional Tibetan medicines for the treatment of chronic wounds	1286:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	7	59	from	wounds	1086:1091	arg1	rats					1096:1099	rats	1096:1099	rats	1096:1099	Moreover, when the TEP-loaded hydrogel was applied to diabetic wounds in rats, it reduced the inflammatory response and improved collagen deposition, which in turn promoted skin healing.					
33881045	1	60	theme	Tibetan	152:158	arg1	TEP					192:194	TEP	192:194	TEP	192:194	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	1	60	theme	Tibetan	152:158	arg1	pills					185:189	The Tibetan eighteen flavor dangshen pills	148:189	The Tibetan eighteen flavor dangshen pills (TEP)	148:195	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	8	61	theme	traditional	1305:1315	arg1	medicines					1325:1333	traditional Tibetan medicines	1305:1333	traditional Tibetan medicines for the treatment of chronic wounds	1305:1369	Our results indicate that TEP-loaded hydrogels may be a new formulation for the application of traditional Tibetan medicines for the treatment of chronic wounds.					
33881045	0	62	theme	Tibetan	85:91	arg1	medicine					93:100	traditional Tibetan medicine	73:100	traditional Tibetan medicine	73:100	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	4	63	theme	alcohol/chitosan	617:632	arg1	hydrogels					643:651	polyvinyl alcohol/chitosan (PVA/CS) hydrogels	607:651	polyvinyl alcohol/chitosan (PVA/CS) hydrogels	607:651	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	4	64	theme	polyvinyl	607:615	arg1	hydrogels					643:651	polyvinyl alcohol/chitosan (PVA/CS) hydrogels	607:651	polyvinyl alcohol/chitosan (PVA/CS) hydrogels	607:651	To improve the utilization rate of TEP in skin treatment, we prepared TEP powder and then introduced it into polyvinyl alcohol/chitosan (PVA/CS) hydrogels to treat diabetic wounds by slowly releasing the active ingredients of TEP.					
33881045	2	65	theme	analgesic	368:376	arg1	effects					378:384	anti-inflammatory and analgesic effects	346:384	anti-inflammatory and analgesic effects	346:384	They have anti-inflammatory and analgesic effects, and healing properties.					
33881045	0	66	theme	traditional	73:83	arg1	medicine					93:100	traditional Tibetan medicine	73:100	traditional Tibetan medicine	73:100	Polyvinyl alcohol/chitosan composite hydrogels with sustained release of traditional Tibetan medicine for promoting chronic diabetic wound healing.					
33881045	1	67	theme	medical	320:326	arg1	system					328:333	the Tibetan medical system	308:333	the Tibetan medical system	308:333	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	1	68	theme	flavor	169:174	arg1	TEP					192:194	TEP	192:194	TEP	192:194	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
33881045	1	68	theme	flavor	169:174	arg1	pills					185:189	The Tibetan eighteen flavor dangshen pills	148:189	The Tibetan eighteen flavor dangshen pills (TEP)	148:195	The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medical system.					
35074102	0	0	theme	decellularized	89:102	arg1	membrane					113:120	decellularized amniotic membrane	89:120	decellularized amniotic membrane	89:120	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	7	1	contain	had	980:982	arg1	sample					973:978	This sample	968:978	This sample	968:978	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	7	1	contain	had	980:982	arg2	adhesion					1015:1022	the most negligible blood cell adhesion	984:1022	the most negligible blood cell adhesion	984:1022	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	2	2	dep	fabrication	272:282	arg1	the					268:270	the	268:270	the	268:270	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	7	3	dep	hemolysis	1042:1050	arg1	%					1040:1040	%	1040:1040	%	1040:1040	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	6	4	theme	MTT	895:897	arg1	results					899:905	our MTT results	891:905	our MTT results	891:905	The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion.					
35074102	3	5	theme	human-AM	554:561	arg1	layer					530:534	a layer	528:534	a layer of decellularized human-AM	528:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	3	6	theme	H2O2-loaded	388:398	arg1	PLA					417:419	PLA	417:419	PLA	417:419	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	3	6	theme	H2O2-loaded	388:398	arg1	acid					411:414	H2O2-loaded polylactic acid	388:414	H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM	388:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	0	7	theme	amniotic	104:111	arg1	membrane					113:120	decellularized amniotic membrane	89:120	decellularized amniotic membrane	89:120	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	1	8	used	utilized	155:162	arg2	membrane					132:139	Amniotic membrane	123:139	Amniotic membrane (AM)	123:144	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	1	8	used	utilized	155:162	arg2	dressing					175:182	a wound dressing	167:182	a wound dressing	167:182	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	1	8	used	utilized	155:162	arg2	AM					142:143	AM	142:143	AM	142:143	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	4	9	theme	4.48 ± 1.8 μm	601:613	arg1	oxygen					677:682	generated oxygen	667:682	generated oxygen	667:682	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	9	theme	4.48 ± 1.8 μm	601:613	arg1	diameter					589:596	a diameter	587:596	a diameter of 4.48 ± 1.8 μm	587:613	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	9	theme	4.48 ± 1.8 μm	601:613	arg1	efficiency					633:642	an encapsulation efficiency	616:642	an encapsulation efficiency of 44.172 ± 4.49%	616:660	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	2	10	from	importance	207:216	arg1	healing					237:243	wound healing	231:243	wound healing	231:243	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	8	11	theme	desirable	1099:1107	arg1	dressing					1174:1181	an active wound dressing	1158:1181	an active wound dressing	1158:1181	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	11	theme	desirable	1099:1107	arg1	properties					1144:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	12	theme	physical	1135:1142	arg1	dressing					1174:1181	an active wound dressing	1158:1181	an active wound dressing	1158:1181	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	12	theme	physical	1135:1142	arg1	properties					1144:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	3	13	theme	decellularized	539:552	arg1	human-AM					554:561	decellularized human-AM	539:561	decellularized human-AM	539:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	1	14	theme	wound	169:173	arg1	dressing					175:182	a wound dressing	167:182	a wound dressing	167:182	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	1	14	theme	wound	169:173	arg1	membrane					132:139	Amniotic membrane	123:139	Amniotic membrane (AM)	123:144	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	0	15	theme	hybrid	2:7	arg1	dressing					33:40	A hybrid oxygen-generating wound dressing	0:40	A hybrid oxygen-generating wound dressing	0:40	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	4	16	theme	generated	667:675	arg1	oxygen					677:682	generated oxygen	667:682	generated oxygen	667:682	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	17	contain	had	583:585	arg1	microparticles					568:581	The microparticles	564:581	The microparticles	564:581	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	17	contain	had	583:585	arg2	oxygen					677:682	generated oxygen	667:682	generated oxygen	667:682	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	17	contain	had	583:585	arg2	efficiency					633:642	an encapsulation efficiency	616:642	an encapsulation efficiency of 44.172 ± 4.49%	616:660	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	17	contain	had	583:585	arg2	diameter					589:596	a diameter	587:596	a diameter of 4.48 ± 1.8 μm	587:613	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	5	18	theme	pore	799:802	arg1	size					804:807	a pore size	797:807	a pore size of 46.72 ± 26.21 μm	797:827	The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm.					
35074102	8	19	theme	chemical	1121:1128	arg1	dressing					1174:1181	an active wound dressing	1158:1181	an active wound dressing	1158:1181	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	19	theme	chemical	1121:1128	arg1	properties					1144:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	0	20	theme	wound	27:31	arg1	dressing					33:40	A hybrid oxygen-generating wound dressing	0:40	A hybrid oxygen-generating wound dressing	0:40	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	5	21	theme	hybrid	709:714	arg1	construct					716:724	The hybrid construct	705:724	The hybrid construct	705:724	The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm.					
35074102	4	22	theme	%	660:660	arg1	oxygen					677:682	generated oxygen	667:682	generated oxygen	667:682	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	22	theme	%	660:660	arg1	diameter					589:596	a diameter	587:596	a diameter of 4.48 ± 1.8 μm	587:613	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	4	22	theme	%	660:660	arg1	efficiency					633:642	an encapsulation efficiency	616:642	an encapsulation efficiency of 44.172 ± 4.49%	616:660	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	0	23	theme	oxygen-generating	9:25	arg1	dressing					33:40	A hybrid oxygen-generating wound dressing	0:40	A hybrid oxygen-generating wound dressing	0:40	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	1	24	theme	Amniotic	123:130	arg1	AM					142:143	AM	142:143	AM	142:143	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	1	24	theme	Amniotic	123:130	arg1	dressing					175:182	a wound dressing	167:182	a wound dressing	167:182	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	1	24	theme	Amniotic	123:130	arg1	membrane					132:139	Amniotic membrane	123:139	Amniotic membrane (AM)	123:144	Amniotic membrane (AM) has been utilized as a wound dressing extensively.					
35074102	3	25	theme	acid	411:414	arg1	microparticles					422:435	H2O2-loaded polylactic acid (PLA) microparticles	388:435	H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM	388:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	6	26	theme	cell	942:945	arg1	growth					947:952	cell growth	942:952	cell growth	942:952	The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion.					
35074102	2	27	theme	wound	231:235	arg1	healing					237:243	wound healing	231:243	wound healing	231:243	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	8	28	theme	wound	1168:1172	arg1	dressing					1174:1181	an active wound dressing	1158:1181	an active wound dressing	1158:1181	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	28	theme	wound	1168:1172	arg1	properties					1144:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	7	29	theme	negligible	993:1002	arg1	adhesion					1015:1022	the most negligible blood cell adhesion	984:1022	the most negligible blood cell adhesion	984:1022	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	0	30	theme	chitosan	51:58	arg1	hydrogel					76:83	chitosan thermosensitive hydrogel	51:83	chitosan thermosensitive hydrogel	51:83	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	8	31	theme	proposed	1078:1085	arg1	structure					1087:1095	the proposed structure	1074:1095	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	5	32	theme	porous	759:764	arg1	structure					766:774	a porous structure	757:774	a porous structure (84.69 ± 8.34%)	757:790	The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm.					
35074102	5	32	theme	porous	759:764	arg1	%					789:789	84.69 ± 8.34%	777:789	84.69 ± 8.34%	777:789	The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm.					
35074102	2	33	theme	oxygen	221:226	arg1	importance					207:216	the importance	203:216	the importance of oxygen in wound healing	203:243	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	7	34	theme	blood	1004:1008	arg1	adhesion					1015:1022	the most negligible blood cell adhesion	984:1022	the most negligible blood cell adhesion	984:1022	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	3	35	theme	thermosensitive	490:504	arg1	hydrogel					506:513	a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel	453:513	a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM	453:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	3	36	theme	polylactic	400:409	arg1	PLA					417:419	PLA	417:419	PLA	417:419	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	3	36	theme	polylactic	400:409	arg1	acid					411:414	H2O2-loaded polylactic acid	388:414	H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM	388:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	6	37	theme	final	916:920	arg1	composite					922:930	the final composite	912:930	the final composite	912:930	The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion.					
35074102	6	38	theme	hydrogel/dAM	834:845	arg1	non-toxic					859:867	non-toxic	859:867	non-toxic	859:867	The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion.					
35074102	6	38	theme	hydrogel/dAM	834:845	arg1	extract					847:853	The hydrogel/dAM extract	830:853	The hydrogel/dAM extract	830:853	The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion.					
35074102	2	39	theme	dressing	335:342	arg1	characterization					288:303	characterization	288:303	characterization	288:303	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	2	39	theme	dressing	335:342	arg1	fabrication					272:282	fabrication	272:282	fabrication	272:282	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	7	40	theme	cell	1010:1013	arg1	adhesion					1015:1022	the most negligible blood cell adhesion	984:1022	the most negligible blood cell adhesion	984:1022	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	2	41	theme	wound	329:333	arg1	dressing					335:342	an oxygen-generating wound dressing	308:342	an oxygen-generating wound dressing	308:342	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	4	42	theme	encapsulation	619:631	arg1	efficiency					633:642	an encapsulation efficiency	616:642	an encapsulation efficiency of 44.172 ± 4.49%	616:660	The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days.					
35074102	7	43	dep	%	1040:1040	arg1	5					1039:1039	5	1039:1039	5	1039:1039	This sample had the most negligible blood cell adhesion with less than 5% hemolysis.					
35074102	2	44	theme	oxygen-generating	311:327	arg1	dressing					335:342	an oxygen-generating wound dressing	308:342	an oxygen-generating wound dressing	308:342	Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM.					
35074102	8	45	theme	biological	1109:1118	arg1	dressing					1174:1181	an active wound dressing	1158:1181	an active wound dressing	1158:1181	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	45	theme	biological	1109:1118	arg1	properties					1144:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	5	46	theme	46.72 ± 26.21 μm	812:827	arg1	size					804:807	a pore size	797:807	a pore size of 46.72 ± 26.21 μm	797:827	The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm.					
35074102	3	47	theme	embedded	437:444	arg1	microparticles					422:435	H2O2-loaded polylactic acid (PLA) microparticles	388:435	H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM	388:561	This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM.					
35074102	0	48	theme	thermosensitive	60:74	arg1	hydrogel					76:83	chitosan thermosensitive hydrogel	51:83	chitosan thermosensitive hydrogel	51:83	A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane.					
35074102	8	49	theme	active	1161:1166	arg1	dressing					1174:1181	an active wound dressing	1158:1181	an active wound dressing	1158:1181	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	8	49	theme	active	1161:1166	arg1	properties					1144:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	the proposed structure's desirable biological, chemical, and physical properties	1074:1153	Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.					
35074102	5	50	dep	formed	730:735	arg1	had					753:755	had	753:755	had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm	753:827	The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm.					
32919697	2	0	theme	sulfonate	646:654	arg1	degrees					656:662	the uncontrollable sulfonate degrees	627:662	especially the uncontrollable sulfonate degrees	616:662	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	4	1	theme	relevant	1117:1124	arg1	process					1137:1143	the relevant regulation process	1113:1143	the relevant regulation process of GAG analogues on cell fate	1113:1173	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	4	1	theme	relevant	1117:1124	arg1	well					1206:1209	well	1206:1209	well	1206:1209	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	2	2	dep	structure	575:583	arg1	the					561:563	the	561:563	the	561:563	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	2	2	dep	structure	575:583	arg1	degrees					656:662	the uncontrollable sulfonate degrees	627:662	especially the uncontrollable sulfonate degrees	616:662	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	5	3	from	units	1323:1327	arg1	networks					1336:1343	the networks	1332:1343	the networks	1332:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	3	4	theme	bionic	763:768	arg1	synthesis					770:778	recent bionic synthesis	756:778	recent bionic synthesis of artificial GAGs analogues at the molecular level	756:830	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	5	5	theme	electron	1493:1500	arg1	SEM					1514:1516	SEM	1514:1516	SEM	1514:1516	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	5	5	theme	electron	1493:1500	arg1	microscope					1502:1511	scanning electron microscope	1484:1511	scanning electron microscope (SEM)	1484:1517	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	1	6	from	regulation	391:400	arg1	organisms					475:483	organisms	475:483	organisms	475:483	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	6	7	theme	prominent	1648:1656	arg1	cytocompatibility					1658:1674	prominent cytocompatibility	1648:1674	prominent cytocompatibility	1648:1674	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	1	8	theme	glycosaminoglycans	200:217	arg1	representative					174:187	a typical representative	164:187	a typical representative of crucial glycosaminoglycans (GAGs)	164:224	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	8	theme	glycosaminoglycans	200:217	arg1	bioactivity					372:382	peculiar bioactivity	363:382	peculiar bioactivity	363:382	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	9	theme	sulfonate	1781:1789	arg1	units					1791:1795	sulfonate units	1781:1795	sulfonate units	1781:1795	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	10	theme	stem	1917:1920	arg1	cells					1922:1926	bone marrow mesenchymal stem cells	1893:1926	bone marrow mesenchymal stem cells (BMSCs)	1893:1934	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	10	theme	stem	1917:1920	arg1	BMSCs					1929:1933	BMSCs	1929:1933	BMSCs	1929:1933	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	3	11	theme	GAGs	794:797	arg1	analogues					799:807	artificial GAGs analogues	783:807	artificial GAGs analogues	783:807	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	5	12	from	contents	1286:1293	arg1	networks					1336:1343	the networks	1332:1343	the networks	1332:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	7	13	theme	saccharide	1766:1775	arg1	proportion					1752:1761	a certain proportion	1742:1761	a certain proportion of saccharide and sulfonate units	1742:1795	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	14	theme	bone	1893:1896	arg1	marrow					1898:1903	bone marrow	1893:1903	bone marrow mesenchymal stem cells (BMSCs)	1893:1934	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	9	15	theme	versatile	2381:2389	arg1	hydrogels					2403:2411	versatile CS-analogue hydrogels	2381:2411	versatile CS-analogue hydrogels	2381:2411	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	8	16	dep	the	2187:2189	arg1	help					2191:2194	help	2191:2194	help	2191:2194	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	3	17	theme	molecular	816:824	arg1	level					826:830	the molecular level	812:830	the molecular level	812:830	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	1	18	theme	fates	425:429	arg1	regulation					391:400	the regulation	387:400	the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms	387:483	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	3	19	theme	cellular	914:921	arg1	behaviors					923:931	the cellular behaviors	910:931	the cellular behaviors	910:931	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	2	20	theme	inhomogeneous	589:601	arg1	composition					603:613	inhomogeneous composition	589:613	inhomogeneous composition	589:613	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	3	21	theme	efficient	857:865	arg1	strategy					867:874	an efficient strategy	854:874	an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates	854:941	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	8	22	theme	different	2099:2107	arg1	conditions					2117:2126	different culture conditions	2099:2126	different culture conditions	2099:2126	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	1	23	theme	e.g.	432:435	arg1	cells					405:409	cells	405:409	cells	405:409	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	23	theme	e.g.	432:435	arg1	proliferation					437:449	e.g. proliferation	432:449	e.g. proliferation	432:449	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	9	24	theme	application	2434:2444	arg1	prospects					2446:2454	promising application prospects	2424:2454	promising application prospects	2424:2454	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	5	25	theme	diverse	1278:1284	arg1	contents					1286:1293	diverse contents	1278:1293	diverse contents of saccharide and sulfonate units in the networks	1278:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	4	26	from	process	1137:1143	arg1	fate					1170:1173	cell fate	1165:1173	cell fate	1165:1173	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	2	27	theme	intrinsic	505:513	arg1	disadvantages					515:527	some intrinsic disadvantages	500:527	some intrinsic disadvantages of natural CS	500:541	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	0	28	theme	chondrogenic	93:104	arg1	differentiation					106:120	osteogenic and chondrogenic differentiation	78:120	osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells	78:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	7	29	theme	cultivation	1695:1705	arg1	results					1707:1713	cell cultivation results	1690:1713	cell cultivation results	1690:1713	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	9	30	theme	biomedical	2463:2472	arg1	fields					2487:2492	the biomedical and clinical fields	2459:2492	the biomedical and clinical fields	2459:2492	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	3	31	theme	great	961:965	arg1	challenges					967:976	great challenges	961:976	great challenges	961:976	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	2	32	theme	CS	540:541	arg1	disadvantages					515:527	some intrinsic disadvantages	500:527	some intrinsic disadvantages of natural CS	500:541	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	9	33	contain	have	2419:2422	arg2	prospects					2446:2454	promising application prospects	2424:2454	promising application prospects	2424:2454	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	9	33	contain	have	2419:2422	arg1	hydrogels					2403:2411	versatile CS-analogue hydrogels	2381:2411	versatile CS-analogue hydrogels	2381:2411	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	9	34	theme	clinical	2478:2485	arg1	fields					2487:2492	the biomedical and clinical fields	2459:2492	the biomedical and clinical fields	2459:2492	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	0	35	theme	marrow	130:135	arg1	cells					154:158	bone marrow mesenchymal stem cells	125:158	bone marrow mesenchymal stem cells	125:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	0	36	theme	Biomimetic	0:9	arg1	synthesis					11:19	Biomimetic synthesis	0:19	Biomimetic synthesis of chondroitin sulfate-analogue	0:51	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	5	37	theme	sulfate-analogue	1232:1247	arg1	hydrogels					1263:1271	chondroitin sulfate-analogue (CS-analogue) hydrogels	1220:1271	chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks	1220:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	7	38	theme	preferable	1807:1816	arg1	promotion					1818:1826	preferable promotion	1807:1826	preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs)	1807:1934	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	8	39	theme	remarkable	2255:2264	arg1	capacity					2266:2273	the remarkable capacity	2251:2273	the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs	2251:2345	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	1	40	with	sulfate	239:245	arg1	polysaccharide					268:281	sulfonated polysaccharide	257:281	sulfonated polysaccharide	257:281	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	41	theme	units	1791:1795	arg1	proportion					1752:1761	a certain proportion	1742:1761	a certain proportion of saccharide and sulfonate units	1742:1795	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	0	42	theme	osteogenic	78:87	arg1	differentiation					106:120	osteogenic and chondrogenic differentiation	78:120	osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells	78:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	0	43	theme	sulfate-analogue	36:51	arg1	synthesis					11:19	Biomimetic synthesis	0:19	Biomimetic synthesis of chondroitin sulfate-analogue	0:51	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	1	44	theme	extracellular	323:335	arg1	matrix					337:342	the extracellular matrix	319:342	the extracellular matrix (ECM)	319:348	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	44	theme	extracellular	323:335	arg1	ECM					345:347	ECM	345:347	ECM	345:347	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	3	45	theme	GAGs	1014:1017	arg1	analogues					1019:1027	GAGs analogues	1014:1027	GAGs analogues with special composition and structure	1014:1066	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	8	46	theme	CS-analogue	2223:2233	arg1	hydrogels					2235:2243	cultivation environment CS-analogue hydrogels	2199:2243	cultivation environment CS-analogue hydrogels	2199:2243	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	7	47	theme	cell	2020:2023	arg1	behaviors					2025:2033	cell behaviors	2020:2033	cell behaviors	2020:2033	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	5	48	dep	Fourier	1416:1422	arg1	transform					1424:1432	transform	1424:1432	transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM)	1424:1517	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	4	49	theme	analogues	1152:1160	arg1	process					1137:1143	the relevant regulation process	1113:1143	the relevant regulation process of GAG analogues on cell fate	1113:1173	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	4	49	theme	analogues	1152:1160	arg1	well					1206:1209	well	1206:1209	well	1206:1209	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	5	50	theme	scanning	1484:1491	arg1	SEM					1514:1516	SEM	1514:1516	SEM	1514:1516	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	5	50	theme	scanning	1484:1491	arg1	microscope					1502:1511	scanning electron microscope	1484:1511	scanning electron microscope (SEM)	1484:1517	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	1	51	theme	typical	166:172	arg1	representative					174:187	a typical representative	164:187	a typical representative of crucial glycosaminoglycans (GAGs)	164:224	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	51	theme	typical	166:172	arg1	bioactivity					372:382	peculiar bioactivity	363:382	peculiar bioactivity	363:382	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	4	52	theme	cell	1165:1168	arg1	fate					1170:1173	cell fate	1165:1173	cell fate	1165:1173	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	6	53	theme	mechanical	1568:1577	arg1	properties					1579:1588	proper mechanical properties	1561:1588	proper mechanical properties	1561:1588	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	3	54	theme	special	1034:1040	arg1	composition					1042:1052	special composition	1034:1052	special composition	1034:1052	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	8	55	theme	BMSCs	2341:2345	arg1	differentiation					2322:2336	either chondrogenic or osteogenic differentiation	2288:2336	either chondrogenic or osteogenic differentiation of BMSCs	2288:2345	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	4	56	theme	regulation	1126:1135	arg1	process					1137:1143	the relevant regulation process	1113:1143	the relevant regulation process of GAG analogues on cell fate	1113:1173	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	4	56	theme	regulation	1126:1135	arg1	well					1206:1209	well	1206:1209	well	1206:1209	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	1	57	dep	cells	405:409	arg1	behaviors					411:419	behaviors	411:419	behaviors	411:419	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	57	dep	cells	405:409	arg1	cells					405:409	cells	405:409	cells	405:409	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	57	dep	cells	405:409	arg1	differentiation					455:469	differentiation	455:469	differentiation	455:469	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	57	dep	cells	405:409	arg1	proliferation					437:449	e.g. proliferation	432:449	e.g. proliferation	432:449	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	3	58	theme	recent	756:761	arg1	synthesis					770:778	recent bionic synthesis	756:778	recent bionic synthesis of artificial GAGs analogues at the molecular level	756:830	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	1	59	theme	sulfonated	257:266	arg1	polysaccharide					268:281	sulfonated polysaccharide	257:281	sulfonated polysaccharide	257:281	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	2	60	theme	uncontrollable	631:644	arg1	degrees					656:662	the uncontrollable sulfonate degrees	627:662	especially the uncontrollable sulfonate degrees	616:662	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	6	61	theme	CS-analogue	1534:1544	arg1	hydrogels					1546:1554	CS-analogue hydrogels	1534:1554	CS-analogue hydrogels with proper mechanical properties	1534:1588	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	3	62	with	analogues	1019:1027	arg1	composition					1042:1052	special composition	1034:1052	special composition	1034:1052	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	3	62	with	analogues	1019:1027	arg1	structure					1058:1066	structure	1058:1066	structure	1058:1066	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	5	63	from	networks	1336:1343	arg1	contents					1286:1293	diverse contents	1278:1293	diverse contents of saccharide and sulfonate units in the networks	1278:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	3	64	theme	artificial	783:792	arg1	analogues					799:807	artificial GAGs analogues	783:807	artificial GAGs analogues	783:807	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	6	65	with	hydrogels	1546:1554	arg1	properties					1579:1588	proper mechanical properties	1561:1588	proper mechanical properties	1561:1588	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	1	66	theme	crucial	192:198	arg1	GAGs					220:223	GAGs	220:223	GAGs	220:223	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	66	theme	crucial	192:198	arg1	glycosaminoglycans					200:217	crucial glycosaminoglycans	192:217	crucial glycosaminoglycans (GAGs)	192:224	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	67	theme	cells	1922:1926	arg1	proliferation					1856:1868	proliferation	1856:1868	proliferation	1856:1868	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	67	theme	cells	1922:1926	arg1	spreading					1845:1853	spreading	1845:1853	spreading	1845:1853	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	67	theme	cells	1922:1926	arg1	adhesion					1835:1842	the adhesion	1831:1842	the adhesion	1831:1842	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	67	theme	cells	1922:1926	arg1	differentiation					1874:1888	differentiation	1874:1888	differentiation	1874:1888	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	68	theme	mesenchymal	1905:1915	arg1	cells					1922:1926	bone marrow mesenchymal stem cells	1893:1926	bone marrow mesenchymal stem cells (BMSCs)	1893:1934	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	68	theme	mesenchymal	1905:1915	arg1	BMSCs					1929:1933	BMSCs	1929:1933	BMSCs	1929:1933	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	0	69	theme	cells	154:158	arg1	differentiation					106:120	osteogenic and chondrogenic differentiation	78:120	osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells	78:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	6	70	theme	degradation	1620:1630	arg1	performance					1632:1642	degradation performance	1620:1642	degradation performance	1620:1642	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	2	71	theme	intricate	565:573	arg1	structure					575:583	intricate structure	565:583	intricate structure	565:583	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	3	72	theme	analogues	799:807	arg1	synthesis					770:778	recent bionic synthesis	756:778	recent bionic synthesis of artificial GAGs analogues at the molecular level	756:830	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	8	73	theme	CS-analogue	2071:2081	arg1	hydrogels					2083:2091	CS-analogue hydrogels	2071:2091	CS-analogue hydrogels	2071:2091	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	1	74	theme	cells	405:409	arg1	regulation					391:400	the regulation	387:400	the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms	387:483	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	75	theme	chondroitin	227:237	arg1	CS					248:249	CS	248:249	CS	248:249	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	75	theme	chondroitin	227:237	arg1	sulfate					239:245	chondroitin sulfate	227:245	chondroitin sulfate (CS) with sulfonated polysaccharide in structures	227:295	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	76	theme	certain	1744:1750	arg1	proportion					1752:1761	a certain proportion	1742:1761	a certain proportion of saccharide and sulfonate units	1742:1795	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	9	77	theme	CS-analogue	2391:2401	arg1	hydrogels					2403:2411	versatile CS-analogue hydrogels	2381:2411	versatile CS-analogue hydrogels	2381:2411	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	3	78	dep	strategy	867:874	arg1	reconstruct					879:889	reconstruct	879:889	to reconstruct GAG for regulating the cellular behaviors and fates	876:941	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	1	79	from	sulfate	239:245	arg1	structures					286:295	structures	286:295	structures	286:295	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	80	from	promotion	1818:1826	arg1	proliferation					1856:1868	proliferation	1856:1868	proliferation	1856:1868	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	80	from	promotion	1818:1826	arg1	spreading					1845:1853	spreading	1845:1853	spreading	1845:1853	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	80	from	promotion	1818:1826	arg1	adhesion					1835:1842	the adhesion	1831:1842	the adhesion	1831:1842	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	80	from	promotion	1818:1826	arg1	differentiation					1874:1888	differentiation	1874:1888	differentiation	1874:1888	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	8	81	theme	culture	2109:2115	arg1	conditions					2117:2126	different culture conditions	2099:2126	different culture conditions	2099:2126	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	7	82	theme	marrow	1898:1903	arg1	cells					1922:1926	bone marrow mesenchymal stem cells	1893:1926	bone marrow mesenchymal stem cells (BMSCs)	1893:1934	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	82	theme	marrow	1898:1903	arg1	BMSCs					1929:1933	BMSCs	1929:1933	BMSCs	1929:1933	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	83	theme	CS-analogue	1716:1726	arg1	hydrogel					1728:1735	CS-analogue hydrogel	1716:1735	CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units	1716:1795	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	5	84	dep	transform	1424:1432	arg1	infrared					1434:1441	infrared	1434:1441	transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM)	1424:1517	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	2	85	from	restrictions	685:696	arg1	applications					733:744	applications	733:744	applications	733:744	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	2	85	from	restrictions	685:696	arg1	functions					719:727	its physiological functions	701:727	its physiological functions	701:727	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	9	86	theme	promising	2424:2432	arg1	prospects					2446:2454	promising application prospects	2424:2454	promising application prospects	2424:2454	Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.					
32919697	5	87	theme	units	1323:1327	arg1	contents					1286:1293	diverse contents	1278:1293	diverse contents of saccharide and sulfonate units in the networks	1278:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	1	88	from	fates	425:429	arg1	organisms					475:483	organisms	475:483	organisms	475:483	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	89	with	hydrogel	1728:1735	arg1	proportion					1752:1761	a certain proportion	1742:1761	a certain proportion of saccharide and sulfonate units	1742:1795	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	90	theme	cell	1690:1693	arg1	results					1707:1713	cell cultivation results	1690:1713	cell cultivation results	1690:1713	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	0	91	theme	stem	149:152	arg1	cells					154:158	bone marrow mesenchymal stem cells	125:158	bone marrow mesenchymal stem cells	125:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	3	92	from	level	826:830	arg1	synthesis					770:778	recent bionic synthesis	756:778	recent bionic synthesis of artificial GAGs analogues at the molecular level	756:830	Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM.					
32919697	0	93	theme	bone	125:128	arg1	marrow					130:135	bone marrow	125:135	bone marrow mesenchymal stem cells	125:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	1	94	theme	peculiar	363:370	arg1	representative					174:187	a typical representative	164:187	a typical representative of crucial glycosaminoglycans (GAGs)	164:224	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	1	94	theme	peculiar	363:370	arg1	bioactivity					372:382	peculiar bioactivity	363:382	peculiar bioactivity	363:382	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	2	95	theme	overt	679:683	arg1	restrictions					685:696	overt restrictions	679:696	overt restrictions on its physiological functions and applications	679:744	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	0	96	theme	mesenchymal	137:147	arg1	cells					154:158	bone marrow mesenchymal stem cells	125:158	bone marrow mesenchymal stem cells	125:158	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	2	97	theme	natural	532:538	arg1	CS					540:541	natural CS	532:541	natural CS	532:541	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	2	98	theme	physiological	705:717	arg1	functions					719:727	its physiological functions	701:727	its physiological functions	701:727	Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications.					
32919697	0	99	theme	chondroitin	24:34	arg1	sulfate-analogue					36:51	chondroitin sulfate-analogue	24:51	chondroitin sulfate-analogue	24:51	Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells.					
32919697	8	100	theme	chondrogenic	2295:2306	arg1	differentiation					2322:2336	either chondrogenic or osteogenic differentiation	2288:2336	either chondrogenic or osteogenic differentiation of BMSCs	2288:2345	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	5	101	theme	zeta	1465:1468	arg1	potential					1470:1478	zeta potential	1465:1478	zeta potential	1465:1478	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	1	102	from	cells	405:409	arg1	organisms					475:483	organisms	475:483	organisms	475:483	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	7	103	theme	sulfonate	1990:1998	arg1	units					2000:2004	saccharide and sulfonate units	1975:2004	units	2000:2004	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	8	104	theme	osteogenic	2311:2320	arg1	differentiation					2322:2336	either chondrogenic or osteogenic differentiation	2288:2336	either chondrogenic or osteogenic differentiation of BMSCs	2288:2345	Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs.					
32919697	5	105	with	hydrogels	1263:1271	arg1	contents					1286:1293	diverse contents	1278:1293	diverse contents of saccharide and sulfonate units in the networks	1278:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	6	106	theme	favorable	1600:1608	arg1	swelling					1610:1617	favorable swelling	1600:1617	favorable swelling	1600:1617	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	5	107	theme	chondroitin	1220:1230	arg1	CS-analogue					1250:1260	CS-analogue	1250:1260	CS-analogue	1250:1260	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	5	107	theme	chondroitin	1220:1230	arg1	sulfate-analogue					1232:1247	chondroitin sulfate-analogue	1220:1247	chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks	1220:1343	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	7	108	theme	units	2000:2004	arg1	significance					1959:1970	the significance	1955:1970	the significance of saccharide and sulfonate units in regulating cell behaviors	1955:2033	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	7	109	theme	saccharide	1975:1984	arg1	units					2000:2004	saccharide and sulfonate units	1975:2004	units	2000:2004	According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors.					
32919697	1	110	from	organisms	475:483	arg1	regulation					391:400	the regulation	387:400	the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms	387:483	As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms.					
32919697	4	111	theme	GAG	1148:1150	arg1	analogues					1152:1160	GAG analogues	1148:1160	GAG analogues	1148:1160	Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well.					
32919697	5	112	theme	FT-IR	1444:1448	arg1	spectroscopy					1451:1462	(FT-IR) spectroscopy	1443:1462	(FT-IR) spectroscopy	1443:1462	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	5	113	theme	saccharide	1298:1307	arg1	units					1323:1327	saccharide and sulfonate units	1298:1327	units	1323:1327	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32919697	6	114	theme	proper	1561:1566	arg1	properties					1579:1588	proper mechanical properties	1561:1588	proper mechanical properties	1561:1588	Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility.					
32919697	5	115	theme	sulfonate	1313:1321	arg1	units					1323:1327	saccharide and sulfonate units	1298:1327	units	1323:1327	Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM).					
32663564	2	0	dep	artichoke	638:646	arg1	leaves					648:653	leaves	648:653	leaves	648:653	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	5	1	theme	A.	1118:1119	arg1	inulinase					1142:1150	A. wewitschiae MN056175 inulinase	1118:1150	A. wewitschiae MN056175 inulinase	1118:1150	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	6	2	theme	total	1278:1282	arg1	carbohydrate					1284:1295	total carbohydrate	1278:1295	total carbohydrate	1278:1295	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	2	3	theme	MN056175	407:414	arg1	isolation					370:378	isolation	370:378	isolation of Aspergillus wewitschiae MN056175	370:414	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	6	4	theme	different	1324:1332	arg1	conditions					1334:1343	different conditions	1324:1343	different conditions designed by central composite design (CCD)	1324:1386	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	5	5	theme	wewitschiae	1121:1131	arg1	inulinase					1142:1150	A. wewitschiae MN056175 inulinase	1118:1150	A. wewitschiae MN056175 inulinase	1118:1150	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	6	6	dep	scolymus	1212:1219	arg1	leaves					1221:1226	leaves	1221:1226	leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD)	1221:1386	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	7	theme	different	1264:1272	arg1	DP					1274:1275	different DP	1264:1275	different DP	1264:1275	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	8	8	theme	negative	1631:1638	arg1	bacteria					1640:1647	gram negative bacteria	1626:1647	gram negative bacteria	1626:1647	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	6	9	theme	composite	1365:1373	arg1	CCD					1383:1385	CCD	1383:1385	CCD	1383:1385	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	9	theme	composite	1365:1373	arg1	design					1375:1380	central composite design	1357:1380	central composite design (CCD)	1357:1386	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	3	10	theme	inulin	746:751	arg1	concentration					753:765	inulin concentration	746:765	inulin concentration	746:765	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	8	11	theme	gram	1626:1629	arg1	bacteria					1640:1647	gram negative bacteria	1626:1647	gram negative bacteria	1626:1647	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	7	12	theme	different	1410:1418	arg1	activities					1451:1460	different high antioxidant and prebiotic activities	1410:1460	different high antioxidant and prebiotic activities	1410:1460	The 15 AL FOS showed different high antioxidant and prebiotic activities.					
32663564	6	13	with	FOS	1255:1257	arg1	DP					1274:1275	different DP	1264:1275	different DP	1264:1275	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	13	with	FOS	1255:1257	arg1	carbohydrate					1284:1295	total carbohydrate	1278:1295	total carbohydrate	1278:1295	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	13	with	FOS	1255:1257	arg1	content					1310:1316	protein content	1302:1316	protein content	1302:1316	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	1	14	theme	global	312:317	arg1	attention					319:327	global attention	312:327	global attention	312:327	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	5	15	from	60 °C	1079:1083	arg1	D-value					1039:1045	D-value	1039:1045	D-value	1039:1045	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	ΔS°					1061:1063	ΔS°	1061:1063	ΔS°	1061:1063	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	73.21 kJ·mol-1					1013:1026	73.21 kJ·mol-1	1013:1026	73.21 kJ·mol-1	1013:1026	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	thermodynamics					888:901	The thermodynamics	884:901	The thermodynamics including	884:911	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	Kd					1029:1030	Kd	1029:1030	Kd	1029:1030	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	ΔH°					1048:1050	ΔH°	1048:1050	ΔH°	1048:1050	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	ΔG°					1053:1055	ΔG°	1053:1055	ΔG°	1053:1055	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	21.82					1003:1007	21.82	1003:1007	21.82	1003:1007	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	15	from	60 °C	1079:1083	arg1	T1/2					1033:1036	T1/2	1033:1036	T1/2	1033:1036	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	7	16	theme	prebiotic	1441:1449	arg1	activities					1451:1460	different high antioxidant and prebiotic activities	1410:1460	different high antioxidant and prebiotic activities	1410:1460	The 15 AL FOS showed different high antioxidant and prebiotic activities.					
32663564	6	17	theme	Cynara	1205:1210	arg1	scolymus					1212:1219	Cynara scolymus	1205:1219	Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD)	1205:1386	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	5	18	theme	activation	945:954	arg1	energy					956:961	activation energy	945:961	activation energy for denaturation	945:978	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	3	19	dep	60 °C	736:740	arg1	%					768:768	1%	767:768	1%	767:768	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	2	20	theme	bioactive	569:577	arg1	FOS					604:606	FOS	604:606	FOS	604:606	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	2	20	theme	bioactive	569:577	arg1	fructo-oligosaccharides					579:601	extract bioactive fructo-oligosaccharides	561:601	extract bioactive fructo-oligosaccharides (FOS)	561:607	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	8	21	theme	antimicrobial	1521:1533	arg1	activity					1535:1542	significant antimicrobial activity	1509:1542	significant antimicrobial activity against tested gram positive bacteria	1509:1580	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	3	22	dep	A.	661:662	arg1	wewitschiae					664:674	wewitschiae	664:674	wewitschiae	664:674	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	1	23	theme	agricultural	204:215	arg1	wastes					217:222	agricultural wastes	204:222	agricultural wastes	204:222	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	0	24	theme	prebiotic	115:123	arg1	scolymus					148:155	prebiotic and antioxidant Cynara scolymus	115:155	prebiotic and antioxidant Cynara scolymus	115:155	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	0	25	from	studies	42:48	arg1	MN056175					77:84	Aspergillus wewitschiae MN056175	53:84	Aspergillus wewitschiae MN056175	53:84	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	5	26	from	50	1072:1073	arg1	D-value					1039:1045	D-value	1039:1045	D-value	1039:1045	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	ΔS°					1061:1063	ΔS°	1061:1063	ΔS°	1061:1063	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	73.21 kJ·mol-1					1013:1026	73.21 kJ·mol-1	1013:1026	73.21 kJ·mol-1	1013:1026	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	thermodynamics					888:901	The thermodynamics	884:901	The thermodynamics including	884:911	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	Kd					1029:1030	Kd	1029:1030	Kd	1029:1030	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	ΔH°					1048:1050	ΔH°	1048:1050	ΔH°	1048:1050	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	ΔG°					1053:1055	ΔG°	1053:1055	ΔG°	1053:1055	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	21.82					1003:1007	21.82	1003:1007	21.82	1003:1007	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	26	from	50	1072:1073	arg1	T1/2					1033:1036	T1/2	1033:1036	T1/2	1033:1036	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	1	27	theme	wastes	217:222	arg1	Utilization					189:199	Utilization	189:199	Utilization of agricultural wastes as cheap natural resources for production of bioactive products	189:286	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	0	28	theme	Physiochemical	0:13	arg1	studies					42:48	Physiochemical, kinetic and thermodynamic studies	0:48	studies	42:48	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	8	29	theme	positive	1564:1571	arg1	bacteria					1573:1580	tested gram positive bacteria	1552:1580	tested gram positive bacteria	1552:1580	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	5	30	theme	activation	918:927	arg1	Ea					914:915	Ea	914:915	Ea (activation energy)	914:935	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	30	theme	activation	918:927	arg1	energy					929:934	activation energy	918:934	activation energy	918:934	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	7	31	theme	AL	1396:1397	arg1	FOS					1399:1401	The 15 AL FOS	1389:1401	The 15 AL FOS	1389:1401	The 15 AL FOS showed different high antioxidant and prebiotic activities.					
32663564	1	32	theme	cheap	227:231	arg1	resources					241:249	cheap natural resources	227:249	cheap natural resources for production of bioactive products	227:286	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	8	33	theme	gram	1559:1562	arg1	bacteria					1573:1580	tested gram positive bacteria	1552:1580	tested gram positive bacteria	1552:1580	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	0	34	theme	Cynara	141:146	arg1	scolymus					148:155	prebiotic and antioxidant Cynara scolymus	115:155	prebiotic and antioxidant Cynara scolymus	115:155	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	2	35	dep	Aspergillus	383:393	arg1	wewitschiae					395:405	wewitschiae	395:405	wewitschiae	395:405	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	2	36	dep	investigating	457:469	arg1	leaves					630:635	leaves	630:635	investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves	457:635	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	1	37	theme	natural	233:239	arg1	resources					241:249	cheap natural resources	227:249	cheap natural resources for production of bioactive products	227:286	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	0	38	theme	kinetic	16:22	arg1	studies					42:48	Physiochemical, kinetic and thermodynamic studies	0:48	studies	42:48	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	0	39	theme	antioxidant	129:139	arg1	scolymus					148:155	prebiotic and antioxidant Cynara scolymus	115:155	prebiotic and antioxidant Cynara scolymus	115:155	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	8	40	theme	tested	1552:1557	arg1	bacteria					1573:1580	tested gram positive bacteria	1552:1580	tested gram positive bacteria	1552:1580	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	2	41	theme	physiochemical	471:484	arg1	kinetics					487:494	kinetics	487:494	kinetics	487:494	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	3	42	theme	maximum	704:710	arg1	activity					712:719	the maximum activity	700:719	the maximum activity	700:719	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	0	43	dep	Aspergillus	53:63	arg1	wewitschiae					65:75	wewitschiae	65:75	wewitschiae	65:75	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	7	44	theme	high	1420:1423	arg1	activities					1451:1460	different high antioxidant and prebiotic activities	1410:1460	different high antioxidant and prebiotic activities	1410:1460	The 15 AL FOS showed different high antioxidant and prebiotic activities.					
32663564	2	45	theme	inulinase	531:539	arg1	thermodynamics					500:513	thermodynamics	500:513	thermodynamics	500:513	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	2	45	theme	inulinase	531:539	arg1	ability					550:556	its ability	546:556	its ability to extract bioactive fructo-oligosaccharides (FOS)	546:607	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	2	45	theme	inulinase	531:539	arg1	kinetics					487:494	kinetics	487:494	kinetics	487:494	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	5	46	theme	inulinase	1142:1150	arg1	stability					1105:1113	the stability	1101:1113	the stability of A. wewitschiae MN056175 inulinase	1101:1150	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	0	47	theme	thermodynamic	28:40	arg1	studies					42:48	Physiochemical, kinetic and thermodynamic studies	0:48	studies	42:48	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	6	48	with	sugar	1242:1246	arg1	DP					1274:1275	different DP	1264:1275	different DP	1264:1275	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	48	with	sugar	1242:1246	arg1	carbohydrate					1284:1295	total carbohydrate	1278:1295	total carbohydrate	1278:1295	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	48	with	sugar	1242:1246	arg1	content					1310:1316	protein content	1302:1316	protein content	1302:1316	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	2	49	theme	extract	561:567	arg1	FOS					604:606	FOS	604:606	FOS	604:606	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	2	49	theme	extract	561:567	arg1	fructo-oligosaccharides					579:601	extract bioactive fructo-oligosaccharides	561:601	extract bioactive fructo-oligosaccharides (FOS)	561:607	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	0	50	theme	Aspergillus	53:63	arg1	MN056175					77:84	Aspergillus wewitschiae MN056175	53:84	Aspergillus wewitschiae MN056175	53:84	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	6	51	theme	protein	1302:1308	arg1	content					1310:1316	protein content	1302:1316	protein content	1302:1316	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	5	52	from	40	1068:1069	arg1	D-value					1039:1045	D-value	1039:1045	D-value	1039:1045	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	ΔS°					1061:1063	ΔS°	1061:1063	ΔS°	1061:1063	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	73.21 kJ·mol-1					1013:1026	73.21 kJ·mol-1	1013:1026	73.21 kJ·mol-1	1013:1026	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	thermodynamics					888:901	The thermodynamics	884:901	The thermodynamics including	884:911	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	Kd					1029:1030	Kd	1029:1030	Kd	1029:1030	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	ΔH°					1048:1050	ΔH°	1048:1050	ΔH°	1048:1050	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	ΔG°					1053:1055	ΔG°	1053:1055	ΔG°	1053:1055	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	21.82					1003:1007	21.82	1003:1007	21.82	1003:1007	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	5	52	from	40	1068:1069	arg1	T1/2					1033:1036	T1/2	1033:1036	T1/2	1033:1036	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	8	53	theme	probiotic	1480:1488	arg1	bacteria					1490:1497	probiotic bacteria	1480:1497	probiotic bacteria	1480:1497	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	7	54	theme	antioxidant	1425:1435	arg1	activities					1451:1460	different high antioxidant and prebiotic activities	1410:1460	different high antioxidant and prebiotic activities	1410:1460	The 15 AL FOS showed different high antioxidant and prebiotic activities.					
32663564	2	55	theme	Aspergillus	383:393	arg1	MN056175					407:414	Aspergillus wewitschiae MN056175	383:414	Aspergillus wewitschiae MN056175	383:414	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	0	56	theme	scolymus	148:155	arg1	extraction					101:110	extraction	101:110	extraction of prebiotic and antioxidant Cynara scolymus	101:155	Physiochemical, kinetic and thermodynamic studies on Aspergillus wewitschiae MN056175 inulinase with extraction of prebiotic and antioxidant Cynara scolymus leaves fructo-oligosaccharides.					
32663564	8	57	theme	Central	1463:1469	arg1	FOS					1471:1473	Central FOS	1463:1473	Central FOS with probiotic bacteria	1463:1497	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	2	58	theme	Cynara	614:619	arg1	scolymus					621:628	Cynara scolymus	614:628	Cynara scolymus	614:628	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	3	59	theme	temperature	724:734	arg1	60 °C					736:740	temperature 60 °C	724:740	temperature 60 °C	724:740	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	8	60	theme	higher	1591:1596	arg1	way					1587:1589	a way	1585:1589	a way higher than those recorded against gram negative bacteria	1585:1647	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	2	61	dep	focused	359:365	arg1	artichoke					638:646	artichoke	638:646	artichoke	638:646	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	5	62	dep	Ed	941:942	arg1	energy					956:961	activation energy	945:961	activation energy for denaturation	945:978	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	3	63	theme	MN056175	676:683	arg1	inulinase					685:693	A. wewitschiae MN056175 inulinase	661:693	A. wewitschiae MN056175 inulinase	661:693	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	1	64	theme	bioactive	269:277	arg1	products					279:286	bioactive products	269:286	bioactive products	269:286	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	8	65	theme	significant	1509:1519	arg1	activity					1535:1542	significant antimicrobial activity	1509:1542	significant antimicrobial activity against tested gram positive bacteria	1509:1580	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	5	66	theme	MN056175	1133:1140	arg1	inulinase					1142:1150	A. wewitschiae MN056175 inulinase	1118:1150	A. wewitschiae MN056175 inulinase	1118:1150	The thermodynamics including, Ea (activation energy) and Ed (activation energy for denaturation) were determined to be 21.82 and 73.21 kJ·mol-1, Kd, T1/2, D-value, ΔH°, ΔG° and ΔS° at 40, 50 and 60 °C which indicated the stability of A. wewitschiae MN056175 inulinase.					
32663564	2	67	theme	promising	419:427	arg1	producer					429:436	promising producer	419:436	promising producer of inulinase	419:449	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	2	68	theme	inulinase	441:449	arg1	producer					429:436	promising producer	419:436	promising producer of inulinase	419:449	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	1	69	theme	products	279:286	arg1	production					255:264	production	255:264	production of bioactive products	255:286	Utilization of agricultural wastes as cheap natural resources for production of bioactive products is currently attracting global attention.					
32663564	2	70	theme	obtained	522:529	arg1	inulinase					531:539	the obtained inulinase	518:539	the obtained inulinase	518:539	For this purpose, this study focused on isolation of Aspergillus wewitschiae MN056175 as promising producer of inulinase, then investigating physiochemical, kinetics and thermodynamics of the obtained inulinase, and its ability to extract bioactive fructo-oligosaccharides (FOS) from Cynara scolymus leaves (artichoke leaves, AL).					
32663564	3	71	theme	A.	661:662	arg1	inulinase					685:693	A. wewitschiae MN056175 inulinase	661:693	A. wewitschiae MN056175 inulinase	661:693	A. wewitschiae MN056175 inulinase gave the maximum activity at temperature 60 °C and inulin concentration 1%.					
32663564	8	72	with	FOS	1471:1473	arg1	bacteria					1490:1497	probiotic bacteria	1480:1497	probiotic bacteria	1480:1497	Central FOS with probiotic bacteria exhibited significant antimicrobial activity against tested gram positive bacteria in a way higher than those recorded against gram negative bacteria.					
32663564	6	73	theme	central	1357:1363	arg1	CCD					1383:1385	CCD	1383:1385	CCD	1383:1385	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
32663564	6	73	theme	central	1357:1363	arg1	design					1375:1380	central composite design	1357:1380	central composite design (CCD)	1357:1386	Moreover, this inulinase was capable of hydrolyzing Cynara scolymus leaves into reducing sugar and 15 FOS with different DP, total carbohydrate, and protein content under different conditions designed by central composite design (CCD).					
33812637	6	0	theme	cancer	1020:1025	arg1	cells					1027:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	3	1	theme	silk	436:439	arg1	agent					511:515	the reducing and stabilizing agent	482:515	the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility	482:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	1	theme	silk	436:439	arg1	fibroin					441:447	silk fibroin	436:447	silk fibroin	436:447	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	1	theme	silk	436:439	arg1	acetate					463:469	cellulose acetate	453:469	cellulose acetate	453:469	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	6	2	theme	MDA-MB-231	996:1005	arg1	cells					1027:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	4	3	theme	different	624:632	arg1	techniques					675:684	different functional, surface and crystallographic techniques	624:684	different functional, surface and crystallographic techniques	624:684	The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques.					
33812637	1	4	theme	enhanced	199:206	arg1	potential					222:230	enhanced biocompatible potential	199:230	enhanced biocompatible potential	199:230	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	0	5	theme	breast	125:130	arg1	cancer					132:137	MCF-7 breast cancer	119:137	MCF-7 breast cancer cell	119:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	5	6	theme	polymer	697:703	arg1	matrix					705:710	The CA/SF polymer matrix	687:710	The CA/SF polymer matrix	687:710	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	0	7	theme	MCF-7	119:123	arg1	cancer					132:137	MCF-7 breast cancer	119:137	MCF-7 breast cancer cell	119:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	4	8	theme	fabricated	574:583	arg1	nanofiber					597:605	The fabricated CA/SF/Au-Ag nanofiber	570:605	The fabricated CA/SF/Au-Ag nanofiber	570:605	The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques.					
33812637	5	9	theme	86.02 ± 57.35 nm	781:796	arg1	range					772:776	the range	768:776	the range of 86.02 ± 57.35 nm in diameter	768:808	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	2	10	theme	CA/SF/Au-Ag	370:380	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	5	11	from	86.02 ± 57.35 nm	781:796	arg1	diameter					801:808	diameter	801:808	diameter	801:808	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	7	12	theme	safer	1152:1156	arg1	applications					1169:1180	safer anticancer applications	1152:1180	safer anticancer applications	1152:1180	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	1	13	with	development	149:159	arg1	potential					222:230	enhanced biocompatible potential	199:230	enhanced biocompatible potential	199:230	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	6	14	with	effects	970:976	arg1	value					1056:1060	an effective IC50 value	1038:1060	an effective IC50 value	1038:1060	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	1	15	theme	biocompatible	208:220	arg1	potential					222:230	enhanced biocompatible potential	199:230	enhanced biocompatible potential	199:230	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	0	16	theme	cancer	132:137	arg1	cell					139:142	MCF-7 breast cancer cell	119:142	MCF-7 breast cancer cell	119:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	6	17	theme	MCF-7	986:990	arg1	cells					1027:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	5	18	theme	average	874:880	arg1	size					882:885	an average size	871:885	an average size of 17.32 nm and 53.21 nm respectively	871:923	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	5	19	theme	CA/SF	691:695	arg1	matrix					705:710	The CA/SF polymer matrix	687:710	The CA/SF polymer matrix	687:710	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	2	20	theme	nanoparticles	355:367	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	0	21	theme	cellulose	16:24	arg1	nanofiber					70:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber	16:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell	16:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	4	22	theme	CA/SF/Au-Ag	585:595	arg1	nanofiber					597:605	The fabricated CA/SF/Au-Ag nanofiber	570:605	The fabricated CA/SF/Au-Ag nanofiber	570:605	The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques.					
33812637	5	23	theme	fiber	853:857	arg1	matrix					859:864	the fiber matrix	849:864	the fiber matrix	849:864	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	2	24	theme	anticancer	407:416	arg1	applications					418:429	anticancer applications	407:429	anticancer applications	407:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	3	25	theme	Ag+	521:523	arg1	ions					533:536	Ag+ and Au+ ions	521:536	Ag+ and Au+ ions with improved biocompatibility	521:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	26	theme	reducing	486:493	arg1	agent					511:515	the reducing and stabilizing agent	482:515	the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility	482:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	26	theme	reducing	486:493	arg1	fibroin					441:447	silk fibroin	436:447	silk fibroin	436:447	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	26	theme	reducing	486:493	arg1	acetate					463:469	cellulose acetate	453:469	cellulose acetate	453:469	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	5	27	theme	53.21 nm	903:910	arg1	size					882:885	an average size	871:885	an average size of 17.32 nm and 53.21 nm respectively	871:923	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	5	28	from	diameter	801:808	arg1	range					772:776	the range	768:776	the range of 86.02 ± 57.35 nm in diameter	768:808	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	0	29	theme	fibroin/Au-Ag	39:51	arg1	nanofiber					70:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber	16:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell	16:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	4	30	theme	crystallographic	658:673	arg1	techniques					675:684	different functional, surface and crystallographic techniques	624:684	different functional, surface and crystallographic techniques	624:684	The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques.					
33812637	5	31	theme	Au	818:819	arg1	embedded					837:844	embedded	837:844	embedded	837:844	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	5	31	theme	Au	818:819	arg1	NPs					828:830	the Au and Ag NPs	814:830	NPs	828:830	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	5	32	with	embedded	837:844	arg1	size					882:885	an average size	871:885	an average size of 17.32 nm and 53.21 nm respectively	871:923	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	0	33	theme	acetate/silk	26:37	arg1	nanofiber					70:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber	16:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell	16:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	2	34	theme	composite	383:391	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	1	35	theme	effective	247:255	arg1	applications					268:279	effective biomedical applications	247:279	effective biomedical applications	247:279	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	0	36	theme	composite	60:68	arg1	nanofiber					70:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber	16:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell	16:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	5	37	theme	Ag	825:826	arg1	embedded					837:844	embedded	837:844	embedded	837:844	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	5	37	theme	Ag	825:826	arg1	NPs					828:830	the Au and Ag NPs	814:830	NPs	828:830	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	6	38	theme	cytotoxic	960:968	arg1	effects					970:976	the cytotoxic effects	956:976	the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value	956:1060	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	1	39	theme	biomedical	257:266	arg1	applications					268:279	effective biomedical applications	247:279	effective biomedical applications	247:279	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	0	40	theme	hybrid	53:58	arg1	nanofiber					70:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber	16:78	cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell	16:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	6	41	theme	IC50	1051:1054	arg1	value					1056:1060	an effective IC50 value	1038:1060	an effective IC50 value	1038:1060	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	5	42	from	range	772:776	arg1	diameter					801:808	diameter	801:808	diameter	801:808	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	3	43	theme	improved	543:550	arg1	biocompatibility					552:567	improved biocompatibility	543:567	improved biocompatibility	543:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	44	theme	cellulose	453:461	arg1	agent					511:515	the reducing and stabilizing agent	482:515	the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility	482:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	44	theme	cellulose	453:461	arg1	fibroin					441:447	silk fibroin	436:447	silk fibroin	436:447	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	44	theme	cellulose	453:461	arg1	acetate					463:469	cellulose acetate	453:469	cellulose acetate	453:469	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	5	45	theme	17.32 nm	890:897	arg1	size					882:885	an average size	871:885	an average size of 17.32 nm and 53.21 nm respectively	871:923	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	3	46	theme	Au+	529:531	arg1	ions					533:536	Ag+ and Au+ ions	521:536	Ag+ and Au+ ions with improved biocompatibility	521:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	7	47	theme	effective	1129:1137	arg1	material					1139:1146	an effective material	1126:1146	an effective material for safer anticancer applications	1126:1180	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	7	47	theme	effective	1129:1137	arg1	nanofibers					1111:1120	CA/SF/Au-Ag composite nanofibers	1089:1120	CA/SF/Au-Ag composite nanofibers	1089:1120	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	3	48	theme	stabilizing	499:509	arg1	agent					511:515	the reducing and stabilizing agent	482:515	the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility	482:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	48	theme	stabilizing	499:509	arg1	fibroin					441:447	silk fibroin	436:447	silk fibroin	436:447	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	3	48	theme	stabilizing	499:509	arg1	acetate					463:469	cellulose acetate	453:469	cellulose acetate	453:469	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	7	49	theme	anticancer	1158:1167	arg1	applications					1169:1180	safer anticancer applications	1152:1180	safer anticancer applications	1152:1180	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	6	50	theme	breast	1013:1018	arg1	cells					1027:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	5	51	theme	rod-shaped	741:750	arg1	morphology					752:761	rod-shaped morphology	741:761	rod-shaped morphology	741:761	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	4	52	theme	surface	646:652	arg1	techniques					675:684	different functional, surface and crystallographic techniques	624:684	different functional, surface and crystallographic techniques	624:684	The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques.					
33812637	2	53	theme	acetate/gold-silver	335:353	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	5	54	dep	needle	730:735	arg1	the					726:728	the	726:728	the	726:728	The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively.					
33812637	3	55	with	ions	533:536	arg1	biocompatibility					552:567	improved biocompatibility	543:567	improved biocompatibility	543:567	The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility.					
33812637	2	56	theme	fibroin/cellulose	317:333	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	7	57	theme	CA/SF/Au-Ag	1089:1099	arg1	material					1139:1146	an effective material	1126:1146	an effective material for safer anticancer applications	1126:1180	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	7	57	theme	CA/SF/Au-Ag	1089:1099	arg1	nanofibers					1111:1120	CA/SF/Au-Ag composite nanofibers	1089:1120	CA/SF/Au-Ag composite nanofibers	1089:1120	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	0	58	theme	biocidal	93:100	arg1	activity					102:109	enhanced biocidal activity	84:109	enhanced biocidal activity against MCF-7 breast cancer cell	84:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	2	59	theme	silk	312:315	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	7	60	theme	composite	1101:1109	arg1	material					1139:1146	an effective material	1126:1146	an effective material for safer anticancer applications	1126:1180	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	7	60	theme	composite	1101:1109	arg1	nanofibers					1111:1120	CA/SF/Au-Ag composite nanofibers	1089:1120	CA/SF/Au-Ag composite nanofibers	1089:1120	Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.					
33812637	1	61	theme	multifunctional	164:178	arg1	nanomaterials					180:192	multifunctional nanomaterials	164:192	multifunctional nanomaterials	164:192	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	6	62	theme	human	1007:1011	arg1	cells					1027:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	MCF-7 and MDA-MB-231 human breast cancer cells	986:1031	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	0	63	theme	enhanced	84:91	arg1	activity					102:109	enhanced biocidal activity	84:109	enhanced biocidal activity against MCF-7 breast cancer cell	84:142	Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.					
33812637	2	64	theme	electrospun	300:310	arg1	nanofiber					393:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber	300:401	electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications	300:429	Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications.					
33812637	1	65	theme	nanomaterials	180:192	arg1	development					149:159	The development	145:159	The development of multifunctional nanomaterials with enhanced biocompatible potential	145:230	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	1	65	theme	nanomaterials	180:192	arg1	crucial					235:241	crucial	235:241	crucial	235:241	The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications.					
33812637	6	66	theme	effective	1041:1049	arg1	value					1056:1060	an effective IC50 value	1038:1060	an effective IC50 value	1038:1060	Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value.					
33812637	4	67	theme	functional	634:643	arg1	techniques					675:684	different functional, surface and crystallographic techniques	624:684	different functional, surface and crystallographic techniques	624:684	The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques.					
33272758	8	0	theme	uncooked	1083:1090	arg1	samples					1092:1098	uncooked samples	1083:1098	uncooked samples	1083:1098	The digestion rates in cooked samples were higher than those in uncooked samples.					
33272758	9	1	dep	bonding	1257:1263	arg1	e.g.					1243:1246	e.g.	1243:1246	e.g.	1243:1246	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	5	2	theme	In	552:553	arg1	digestion					561:569	In vitro digestion	552:569	In vitro digestion	552:569	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	9	3	theme	chemical	1323:1330	arg1	pronounced					1360:1369	pronounced	1360:1369	pronounced	1360:1369	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	3	theme	chemical	1323:1330	arg1	effect					1332:1337	a chemical effect	1321:1337	a chemical effect from protein	1321:1350	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	4	theme	chemical	1226:1233	arg1	effect					1235:1240	some chemical effect	1221:1240	some chemical effect (e.g. hydrogen bonding between protein and starch)	1221:1291	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	5	5	theme	flour	634:638	arg1	k					616:616	k	616:616	k	616:616	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	5	5	theme	flour	634:638	arg1	coefficients					602:613	the digestion rate coefficients	583:613	the digestion rate coefficients (k) of cooked rice flour	583:638	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	5	5	theme	flour	634:638	arg1	lower					659:663	lower	659:663	lower	659:663	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	9	6	theme	starch	1140:1145	arg1	rates					1157:1161	starch digestion rates	1140:1161	starch digestion rates	1140:1161	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	2	7	from	effects	186:192	arg1	digestibility					235:247	starch in vitro digestibility	219:247	starch in vitro digestibility in cooked and uncooked rice	219:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	0	8	from	Effects	0:6	arg1	rice					34:37	rice	34:37	rice	34:37	Effects of endogenous proteins on rice digestion during small intestine (in vitro) digestion.					
33272758	9	9	theme	cooked	1124:1129	arg1	samples					1131:1137	cooked samples	1124:1137	cooked samples	1124:1137	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	2	10	from	mechanisms	286:295	arg1	digestibility					235:247	starch in vitro digestibility	219:247	starch in vitro digestibility in cooked and uncooked rice	219:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	3	11	theme	endosperm	345:353	arg1	proteins					355:362	rice endosperm proteins	340:362	rice endosperm proteins	340:362	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	4	12	theme	Starch	470:475	arg1	structure					492:500	Starch molecular fine structure	470:500	Starch molecular fine structure	470:500	Starch molecular fine structure was examined using size-exclusion chromatography.					
33272758	6	13	from	SWR4	749:752	arg1	samples					736:742	samples	736:742	samples from SWR4, k	736:755	(e.g for samples from SWR4, k is 9.6, 12.9 and 11.6 × 10-2 min-1 for cooked rice flour, isolated starch and starch-protein mixture, respectively).					
33272758	5	14	theme	cooked	622:627	arg1	flour					634:638	cooked rice flour	622:638	cooked rice flour	622:638	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	2	15	theme	uncooked	263:270	arg1	rice					272:275	uncooked rice	263:275	uncooked rice	263:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	4	16	theme	fine	487:490	arg1	structure					492:500	Starch molecular fine structure	470:500	Starch molecular fine structure	470:500	Starch molecular fine structure was examined using size-exclusion chromatography.					
33272758	5	17	theme	rice	629:632	arg1	flour					634:638	cooked rice flour	622:638	cooked rice flour	622:638	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	7	18	theme	digestion	896:904	arg1	coefficients					911:922	digestion rate coefficients	896:922	digestion rate coefficients	896:922	For uncooked samples, digestion rate coefficients were 1.4, 1.5 and 1.8 × 10-2 min-1 for flour, starch-protein mixture and starch, respectively.					
33272758	4	19	theme	molecular	477:485	arg1	structure					492:500	Starch molecular fine structure	470:500	Starch molecular fine structure	470:500	Starch molecular fine structure was examined using size-exclusion chromatography.					
33272758	1	20	with	Rices	94:98	arg1	contents					120:127	higher protein contents	105:127	higher protein contents	105:127	Rices with higher protein contents are nutritionally desirable.					
33272758	5	21	theme	digestion	587:595	arg1	k					616:616	k	616:616	k	616:616	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	5	21	theme	digestion	587:595	arg1	coefficients					602:613	the digestion rate coefficients	583:613	the digestion rate coefficients (k) of cooked rice flour	583:638	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	5	21	theme	digestion	587:595	arg1	lower					659:663	lower	659:663	lower	659:663	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	3	22	theme	granules	408:415	arg1	morphologies					372:383	the morphologies	368:383	the morphologies of proteins and starch granules	368:415	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	3	22	theme	granules	408:415	arg1	composition					325:335	The composition	321:335	The composition of rice endosperm proteins	321:362	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	0	23	theme	proteins	22:29	arg1	Effects					0:6	Effects	0:6	Effects of endogenous proteins on rice	0:37	Effects of endogenous proteins on rice digestion during small intestine (in vitro) digestion.					
33272758	5	24	theme	rate	597:600	arg1	k					616:616	k	616:616	k	616:616	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	5	24	theme	rate	597:600	arg1	coefficients					602:613	the digestion rate coefficients	583:613	the digestion rate coefficients (k) of cooked rice flour	583:638	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	5	24	theme	rate	597:600	arg1	lower					659:663	lower	659:663	lower	659:663	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	9	25	theme	protein	1188:1194	arg1	barrier					1205:1211	the protein physical barrier	1184:1211	the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch)	1184:1291	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	2	26	from	digestibility	235:247	arg1	rice					272:275	uncooked rice	263:275	uncooked rice	263:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	2	26	from	digestibility	235:247	arg1	cooked					252:257	cooked	252:257	cooked	252:257	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	3	27	theme	proteins	355:362	arg1	morphologies					372:383	the morphologies	368:383	the morphologies of proteins and starch granules	368:415	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	3	27	theme	proteins	355:362	arg1	composition					325:335	The composition	321:335	The composition of rice endosperm proteins	321:362	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	9	28	from	protein	1400:1406	arg1	barrier					1387:1393	a physical barrier	1376:1393	a physical barrier from protein	1376:1406	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	0	29	dep	intestine	62:70	arg1	vitro					76:80	in vitro	73:80	in vitro	73:80	Effects of endogenous proteins on rice digestion during small intestine (in vitro) digestion.					
33272758	5	30	dep	In	552:553	arg1	vitro					555:559	vitro	555:559	vitro	555:559	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	3	31	theme	confocal	449:456	arg1	microscopy					458:467	confocal microscopy	449:467	confocal microscopy	449:467	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	0	32	theme	endogenous	11:20	arg1	proteins					22:29	endogenous proteins	11:29	endogenous proteins	11:29	Effects of endogenous proteins on rice digestion during small intestine (in vitro) digestion.					
33272758	9	33	from	protein	1344:1350	arg1	pronounced					1360:1369	pronounced	1360:1369	pronounced	1360:1369	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	33	from	protein	1344:1350	arg1	effect					1332:1337	a chemical effect	1321:1337	a chemical effect from protein	1321:1350	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	34	theme	physical	1378:1385	arg1	barrier					1387:1393	a physical barrier	1376:1393	a physical barrier from protein	1376:1406	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	5	35	theme	starch-protein	703:716	arg1	mixture					718:724	a starch-protein mixture	701:724	a starch-protein mixture	701:724	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	2	36	theme	in	226:227	arg1	digestibility					235:247	starch in vitro digestibility	219:247	starch in vitro digestibility in cooked and uncooked rice	219:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	2	37	dep	in	226:227	arg1	vitro					229:233	vitro	229:233	vitro	229:233	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	3	38	theme	starch	401:406	arg1	granules					408:415	starch granules	401:415	starch granules	401:415	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	8	39	theme	cooked	1042:1047	arg1	samples					1049:1055	cooked samples	1042:1055	cooked samples	1042:1055	The digestion rates in cooked samples were higher than those in uncooked samples.					
33272758	2	40	theme	starch	219:224	arg1	digestibility					235:247	starch in vitro digestibility	219:247	starch in vitro digestibility in cooked and uncooked rice	219:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	3	41	theme	rice	340:343	arg1	proteins					355:362	rice endosperm proteins	340:362	rice endosperm proteins	340:362	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	9	42	from	pronounced	1360:1369	arg1	samples					1312:1318	uncooked samples	1303:1318	uncooked samples	1303:1318	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	7	43	theme	starch-protein	970:983	arg1	mixture					985:991	starch-protein mixture	970:991	starch-protein mixture	970:991	For uncooked samples, digestion rate coefficients were 1.4, 1.5 and 1.8 × 10-2 min-1 for flour, starch-protein mixture and starch, respectively.					
33272758	3	44	theme	proteins	388:395	arg1	morphologies					372:383	the morphologies	368:383	the morphologies of proteins and starch granules	368:415	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	3	44	theme	proteins	388:395	arg1	composition					325:335	The composition	321:335	The composition of rice endosperm proteins	321:362	The composition of rice endosperm proteins and the morphologies of proteins and starch granules were determined by SDS-PAGE and confocal microscopy.					
33272758	7	45	theme	rate	906:909	arg1	coefficients					911:922	digestion rate coefficients	896:922	digestion rate coefficients	896:922	For uncooked samples, digestion rate coefficients were 1.4, 1.5 and 1.8 × 10-2 min-1 for flour, starch-protein mixture and starch, respectively.					
33272758	5	46	theme	isolated	679:686	arg1	starch					688:693	isolated starch	679:693	isolated starch	679:693	In vitro digestion showed that the digestion rate coefficients (k) of cooked rice flour were significantly lower than those of isolated starch or of a starch-protein mixture.					
33272758	2	47	theme	endosperm	197:205	arg1	proteins					207:214	endosperm proteins	197:214	endosperm proteins	197:214	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	2	48	theme	proteins	207:214	arg1	effects					186:192	the effects	182:192	the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice	182:275	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	2	48	theme	proteins	207:214	arg1	mechanisms					286:295	the mechanisms	282:295	the mechanisms underlying any changes	282:318	This study investigates the effects of endosperm proteins on starch in vitro digestibility in cooked and uncooked rice, and the mechanisms underlying any changes.					
33272758	6	49	theme	starch-protein	835:848	arg1	mixture					850:856	starch-protein mixture	835:856	starch-protein mixture	835:856	(e.g for samples from SWR4, k is 9.6, 12.9 and 11.6 × 10-2 min-1 for cooked rice flour, isolated starch and starch-protein mixture, respectively).					
33272758	9	50	theme	hydrogen	1248:1255	arg1	bonding					1257:1263	hydrogen bonding	1248:1263	hydrogen bonding between protein and starch	1248:1290	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	6	51	theme	rice	803:806	arg1	flour					808:812	cooked rice flour	796:812	cooked rice flour	796:812	(e.g for samples from SWR4, k is 9.6, 12.9 and 11.6 × 10-2 min-1 for cooked rice flour, isolated starch and starch-protein mixture, respectively).					
33272758	1	52	theme	higher	105:110	arg1	contents					120:127	higher protein contents	105:127	higher protein contents	105:127	Rices with higher protein contents are nutritionally desirable.					
33272758	7	53	theme	uncooked	878:885	arg1	samples					887:893	uncooked samples	878:893	uncooked samples	878:893	For uncooked samples, digestion rate coefficients were 1.4, 1.5 and 1.8 × 10-2 min-1 for flour, starch-protein mixture and starch, respectively.					
33272758	9	54	theme	uncooked	1303:1310	arg1	samples					1312:1318	uncooked samples	1303:1318	uncooked samples	1303:1318	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	55	from	samples	1312:1318	arg1	pronounced					1360:1369	pronounced	1360:1369	pronounced	1360:1369	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	55	from	samples	1312:1318	arg1	effect					1332:1337	a chemical effect	1321:1337	a chemical effect from protein	1321:1350	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	6	56	theme	cooked	796:801	arg1	flour					808:812	cooked rice flour	796:812	cooked rice flour	796:812	(e.g for samples from SWR4, k is 9.6, 12.9 and 11.6 × 10-2 min-1 for cooked rice flour, isolated starch and starch-protein mixture, respectively).					
33272758	7	57	theme	1.8 × 10-2	942:951	arg1	min-1					953:957	1.8 × 10-2 min-1	942:957	1.8 × 10-2 min-1	942:957	For uncooked samples, digestion rate coefficients were 1.4, 1.5 and 1.8 × 10-2 min-1 for flour, starch-protein mixture and starch, respectively.					
33272758	1	58	theme	protein	112:118	arg1	contents					120:127	higher protein contents	105:127	higher protein contents	105:127	Rices with higher protein contents are nutritionally desirable.					
33272758	0	59	theme	intestine	62:70	arg1	digestion					83:91	small intestine (in vitro) digestion	56:91	small intestine (in vitro) digestion	56:91	Effects of endogenous proteins on rice digestion during small intestine (in vitro) digestion.					
33272758	8	60	from	rates	1033:1037	arg1	samples					1049:1055	cooked samples	1042:1055	cooked samples	1042:1055	The digestion rates in cooked samples were higher than those in uncooked samples.					
33272758	9	61	theme	physical	1196:1203	arg1	barrier					1205:1211	the protein physical barrier	1184:1211	the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch)	1184:1291	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	9	62	theme	digestion	1147:1155	arg1	rates					1157:1161	starch digestion rates	1140:1161	starch digestion rates	1140:1161	This suggests that, in cooked samples, starch digestion rates are more affected by the protein physical barrier than by some chemical effect (e.g. hydrogen bonding between protein and starch), while in uncooked samples, a chemical effect from protein is more pronounced than a physical barrier from protein.					
33272758	0	63	theme	small	56:60	arg1	intestine					62:70	small intestine	56:70	small intestine (in vitro) digestion	56:91	Effects of endogenous proteins on rice digestion during small intestine (in vitro) digestion.					
33272758	8	64	theme	digestion	1023:1031	arg1	higher					1062:1067	higher	1062:1067	higher	1062:1067	The digestion rates in cooked samples were higher than those in uncooked samples.					
33272758	8	64	theme	digestion	1023:1031	arg1	rates					1033:1037	The digestion rates	1019:1037	The digestion rates in cooked samples	1019:1055	The digestion rates in cooked samples were higher than those in uncooked samples.					
33272758	4	65	theme	size-exclusion	521:534	arg1	chromatography					536:549	size-exclusion chromatography	521:549	size-exclusion chromatography	521:549	Starch molecular fine structure was examined using size-exclusion chromatography.					
33422512	4	0	theme	attained	941:948	arg1	diameters					950:958	enzymatically (ECN) attained diameters	921:958	enzymatically (ECN) attained diameters	921:958	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	1	1	theme	stage-alkaline	347:360	arg1	WS					387:388	WS	387:388	WS	387:388	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	1	theme	stage-alkaline	347:360	arg1	straw					380:384	pre-treated wheat straw	362:384	two- stage-alkaline pre-treated wheat straw (WS)	342:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	5	2	theme	HCN	1073:1075	arg1	nano-applications					1105:1121	the HCN morphology suits homogenous nano-applications	1069:1121	the HCN morphology suits homogenous nano-applications	1069:1121	Thus, the HCN morphology suits homogenous nano-applications, whereas that of the ECN, would suit heterogenous nano-applications.					
33422512	1	3	theme	pre-treated	362:372	arg1	WS					387:388	WS	387:388	WS	387:388	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	3	theme	pre-treated	362:372	arg1	straw					380:384	pre-treated wheat straw	362:384	two- stage-alkaline pre-treated wheat straw (WS)	342:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	2	4	theme	FiberCare	586:594	arg1	dosage					596:601	FiberCare dosage	586:601	FiberCare dosage	586:601	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	3	5	dep	functional	806:815	arg1	crystallinity					818:830	crystallinity	818:830	crystallinity	818:830	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	5	dep	functional	806:815	arg1	degradation					844:854	thermal degradation	836:854	thermal degradation	836:854	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	7	6	theme	thermal	1381:1387	arg1	temperatures					1401:1412	maximum thermal degradation temperatures	1373:1412	maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1373:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	3	7	theme	thermal	836:842	arg1	degradation					844:854	thermal degradation	836:854	thermal degradation	836:854	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	1	8	theme	morphological	267:279	arg1	properties					296:305	morphological and functional properties	267:305	morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	267:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	0	9	theme	pre-treated	106:116	arg1	straw					124:128	alkaline pre-treated wheat straw	97:128	alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes	97:183	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	1	10	theme	wheat	374:378	arg1	WS					387:388	WS	387:388	WS	387:388	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	10	theme	wheat	374:378	arg1	straw					380:384	pre-treated wheat straw	362:384	two- stage-alkaline pre-treated wheat straw (WS)	342:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	6	11	theme	4.64 h	1276:1281	arg1	time					1259:1262	optimal production time	1240:1262	optimal production time of 7.41 and 4.64 h	1240:1281	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	8	12	theme	synergetic	1499:1508	arg1	effects					1510:1516	potential synergetic effects	1489:1516	potential synergetic effects	1489:1516	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	5	13	theme	suits	1088:1092	arg1	nano-applications					1105:1121	the HCN morphology suits homogenous nano-applications	1069:1121	the HCN morphology suits homogenous nano-applications	1069:1121	Thus, the HCN morphology suits homogenous nano-applications, whereas that of the ECN, would suit heterogenous nano-applications.					
33422512	0	14	theme	alkaline	97:104	arg1	straw					124:128	alkaline pre-treated wheat straw	97:128	alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes	97:183	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	7	15	theme	&	1311:1311	arg1	nanocolloids					1355:1366	thermally stable nanocolloids	1338:1366	thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1338:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	7	15	theme	&	1311:1311	arg1	ECN					1313:1315	Both the HCN & ECN	1298:1315	Both the HCN & ECN	1298:1315	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	7	16	with	nanocolloids	1355:1366	arg1	temperatures					1401:1412	maximum thermal degradation temperatures	1373:1412	maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1373:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	8	17	contain	have	1484:1487	arg2	effects					1510:1516	potential synergetic effects	1489:1516	potential synergetic effects	1489:1516	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	8	17	contain	have	1484:1487	arg1	methods					1476:1482	The two CNF production methods	1453:1482	The two CNF production methods	1453:1482	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	7	18	theme	Zeta	1429:1432	arg1	~-16 mV					1444:1450	Zeta potential ~-16 mV	1429:1450	Zeta potential ~-16 mV	1429:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	1	19	theme	functional	285:294	arg1	properties					296:305	morphological and functional properties	267:305	morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	267:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	3	20	theme	CNF	725:727	arg1	yields					729:734	The CNF yields	721:734	The CNF yields	721:734	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	20	theme	CNF	725:727	arg1	properties					857:866	morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties	737:866	morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties	737:866	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	5	21	theme	homogenous	1094:1103	arg1	nano-applications					1105:1121	the HCN morphology suits homogenous nano-applications	1069:1121	the HCN morphology suits homogenous nano-applications	1069:1121	Thus, the HCN morphology suits homogenous nano-applications, whereas that of the ECN, would suit heterogenous nano-applications.					
33422512	4	22	theme	&	1018:1018	arg1	%					1022:1022	514 nm & 70%	1011:1022	514 nm & 70%	1011:1022	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	6	23	theme	optimal	1240:1246	arg1	time					1259:1262	optimal production time	1240:1262	optimal production time of 7.41 and 4.64 h	1240:1281	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	0	24	theme	wheat	118:122	arg1	straw					124:128	alkaline pre-treated wheat straw	97:128	alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes	97:183	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	2	25	theme	endo-1,4-β-glucanase	611:630	arg1	time					568:571	time (4-8 h)	568:579	time (4-8 h)	568:579	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	2	25	theme	endo-1,4-β-glucanase	611:630	arg1	unit/g					632:637	50-100 endo-1,4-β-glucanase unit/g	604:637	50-100 endo-1,4-β-glucanase unit/g	604:637	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	4	26	theme	&	1042:1042	arg1	%					1046:1046	& 48%	1042:1046	& 48%	1042:1046	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	2	27	theme	extraction	396:405	arg1	conditions					407:416	The extraction conditions	392:416	The extraction conditions	392:416	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	2	28	theme	experimental	465:476	arg1	approach					478:485	experimental approach	465:485	experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g)	465:687	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	7	29	theme	HCN	1307:1309	arg1	nanocolloids					1355:1366	thermally stable nanocolloids	1338:1366	thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1338:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	7	29	theme	HCN	1307:1309	arg1	ECN					1313:1315	Both the HCN & ECN	1298:1315	Both the HCN & ECN	1298:1315	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	1	30	from	properties	296:305	arg1	WS					387:388	WS	387:388	WS	387:388	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	30	from	properties	296:305	arg1	straw					380:384	pre-treated wheat straw	362:384	two- stage-alkaline pre-treated wheat straw (WS)	342:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	2	31	theme	β-glucanase	668:678	arg1	time					568:571	time (4-8 h)	568:579	time (4-8 h)	568:579	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	2	31	theme	β-glucanase	668:678	arg1	units/g					680:686	10-20 fungal β-glucanase units/g	655:686	10-20 fungal β-glucanase units/g	655:686	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	0	32	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of physical and functional properties of cellulose	0:70	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	1	33	theme	properties	296:305	arg1	diversification					248:262	diversification	248:262	diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	248:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	3	34	theme	morphological	737:749	arg1	yields					729:734	The CNF yields	721:734	The CNF yields	721:734	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	34	theme	morphological	737:749	arg1	properties					857:866	morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties	737:866	morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties	737:866	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	2	35	theme	fungal	661:666	arg1	time					568:571	time (4-8 h)	568:579	time (4-8 h)	568:579	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	2	35	theme	fungal	661:666	arg1	units/g					680:686	10-20 fungal β-glucanase units/g	655:686	10-20 fungal β-glucanase units/g	655:686	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	4	36	theme	%	1022:1022	arg1	crystallinity					988:1000	crystallinity	988:1000	crystallinity	988:1000	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	36	theme	%	1022:1022	arg1	PdI					971:973	PdI	971:973	PdI	971:973	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	36	theme	%	1022:1022	arg1	length					976:981	length	976:981	length	976:981	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	0	37	theme	physical	24:31	arg1	properties					48:57	physical and functional properties	24:57	physical and functional properties of cellulose	24:70	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	4	38	contain	had	967:969	arg2	length					976:981	length	976:981	length	976:981	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	38	contain	had	967:969	arg2	PdI					971:973	PdI	971:973	PdI	971:973	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	38	contain	had	967:969	arg1	CNF					887:889	The CNF	883:889	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm	883:965	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	38	contain	had	967:969	arg2	crystallinity					988:1000	crystallinity	988:1000	crystallinity	988:1000	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	0	39	theme	optimized	133:141	arg1	acid					156:159	optimized hydrochloric acid	133:159	optimized hydrochloric acid	133:159	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	0	40	dep	nanofibers	72:81	arg1	isolated					83:90	isolated	83:90	nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes	72:183	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	2	41	theme	central	438:444	arg1	composite					446:454	a central composite	436:454	a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment	436:718	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	1	42	theme	cellulose	310:318	arg1	CNF					332:334	CNF	332:334	CNF	332:334	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	42	theme	cellulose	310:318	arg1	nanofibers					320:329	cellulose nanofibers	310:329	cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	310:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	5	43	theme	heterogenous	1160:1171	arg1	nano-applications					1173:1189	heterogenous nano-applications	1160:1189	heterogenous nano-applications	1160:1189	Thus, the HCN morphology suits homogenous nano-applications, whereas that of the ECN, would suit heterogenous nano-applications.					
33422512	7	44	theme	degradation	1389:1399	arg1	temperatures					1401:1412	maximum thermal degradation temperatures	1373:1412	maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1373:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	1	45	theme	enzymatic	207:215	arg1	treatments					217:226	enzymatic treatments	207:226	enzymatic treatments	207:226	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	45	theme	enzymatic	207:215	arg1	methods					190:196	Two methods	186:196	Two methods	186:196	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	0	46	theme	functional	37:46	arg1	properties					48:57	physical and functional properties	24:57	physical and functional properties of cellulose	24:70	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	1	47	theme	nanofibers	320:329	arg1	properties					296:305	morphological and functional properties	267:305	morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	267:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	2	48	theme	HCl-based	544:552	arg1	treatment					554:562	the HCl-based treatment	540:562	the HCl-based treatment	540:562	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	8	49	theme	functional	1556:1565	arg1	properties					1567:1576	functional properties	1556:1576	functional properties	1556:1576	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	8	50	theme	potential	1489:1497	arg1	effects					1510:1516	potential synergetic effects	1489:1516	potential synergetic effects	1489:1516	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	4	51	theme	514 nm	1011:1016	arg1	%					1022:1022	514 nm & 70%	1011:1022	514 nm & 70%	1011:1022	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	5	52	theme	morphology	1077:1086	arg1	nano-applications					1105:1121	the HCN morphology suits homogenous nano-applications	1069:1121	the HCN morphology suits homogenous nano-applications	1069:1121	Thus, the HCN morphology suits homogenous nano-applications, whereas that of the ECN, would suit heterogenous nano-applications.					
33422512	0	53	theme	hydrochloric	143:154	arg1	acid					156:159	optimized hydrochloric acid	133:159	optimized hydrochloric acid	133:159	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	7	54	theme	maximum	1373:1379	arg1	temperatures					1401:1412	maximum thermal degradation temperatures	1373:1412	maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1373:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	1	55	from	straw	380:384	arg1	CNF					332:334	CNF	332:334	CNF	332:334	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	55	from	straw	380:384	arg1	properties					296:305	morphological and functional properties	267:305	morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	267:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	1	55	from	straw	380:384	arg1	nanofibers					320:329	cellulose nanofibers	310:329	cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS)	310:389	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	2	56	theme	enzyme-based	697:708	arg1	treatment					710:718	the enzyme-based treatment	693:718	the enzyme-based treatment	693:718	The extraction conditions were optimized by a central composite designed experimental approach varying time (4-8 h) and temperature (80-120 °C) for the HCl-based treatment and time (4-8 h), and FiberCare dosage (50-100 endo-1,4-β-glucanase unit/g) and Viscozyme (10-20 fungal β-glucanase units/g) for the enzyme-based treatment.					
33422512	6	57	theme	7.41	1267:1270	arg1	time					1259:1262	optimal production time	1240:1262	optimal production time of 7.41 and 4.64 h	1240:1281	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	4	58	theme	0.92	1029:1032	arg1	crystallinity					988:1000	crystallinity	988:1000	crystallinity	988:1000	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	58	theme	0.92	1029:1032	arg1	PdI					971:973	PdI	971:973	PdI	971:973	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	58	theme	0.92	1029:1032	arg1	length					976:981	length	976:981	length	976:981	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	0	59	theme	enzymatic	165:173	arg1	processes					175:183	enzymatic processes	165:183	enzymatic processes	165:183	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	6	60	theme	production	1248:1257	arg1	time					1259:1262	optimal production time	1240:1262	optimal production time of 7.41 and 4.64 h	1240:1281	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	0	61	theme	properties	48:57	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of physical and functional properties of cellulose	0:70	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	3	62	theme	polydispersity	752:765	arg1	length					780:785	length	780:785	length	780:785	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	62	theme	polydispersity	752:765	arg1	index					767:771	polydispersity index	752:771	polydispersity index (PdI)	752:777	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	62	theme	polydispersity	752:765	arg1	diameter					791:798	diameter	791:798	diameter	791:798	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	62	theme	polydispersity	752:765	arg1	PdI					774:776	PdI	774:776	PdI	774:776	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	6	63	theme	HCN	1196:1198	arg1	%					1232:1232	~20%	1229:1232	~20%	1229:1232	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	6	63	theme	HCN	1196:1198	arg1	yields					1208:1213	The HCN and ECN yields	1192:1213	yields	1208:1213	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	6	63	theme	HCN	1196:1198	arg1	similar					1220:1226	similar	1220:1226	similar	1220:1226	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	0	64	from	straw	124:128	arg1	acid					156:159	optimized hydrochloric acid	133:159	optimized hydrochloric acid	133:159	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	0	64	from	straw	124:128	arg1	processes					175:183	enzymatic processes	165:183	enzymatic processes	165:183	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	6	65	dep	similar	1220:1226	arg1	%					1232:1232	~20%	1229:1232	~20%	1229:1232	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	6	65	dep	similar	1220:1226	arg1	yields					1208:1213	The HCN and ECN yields	1192:1213	yields	1208:1213	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	6	65	dep	similar	1220:1226	arg1	similar					1220:1226	similar	1220:1226	similar	1220:1226	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	8	66	theme	CNF	1521:1523	arg1	production					1525:1534	CNF production	1521:1534	CNF production	1521:1534	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	4	67	dep	enzymatically	921:933	arg1	ECN					936:938	ECN	936:938	ECN	936:938	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	6	68	theme	ECN	1204:1206	arg1	%					1232:1232	~20%	1229:1232	~20%	1229:1232	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	6	68	theme	ECN	1204:1206	arg1	yields					1208:1213	The HCN and ECN yields	1192:1213	yields	1208:1213	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	6	68	theme	ECN	1204:1206	arg1	similar					1220:1226	similar	1220:1226	similar	1220:1226	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	4	69	theme	0.53	1005:1008	arg1	crystallinity					988:1000	crystallinity	988:1000	crystallinity	988:1000	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	69	theme	0.53	1005:1008	arg1	PdI					971:973	PdI	971:973	PdI	971:973	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	69	theme	0.53	1005:1008	arg1	length					976:981	length	976:981	length	976:981	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	4	70	dep	HCl	907:909	arg1	~17 nm					960:965	~17 nm	960:965	~17 nm	960:965	The CNF produced by the HCl (HCN) and enzymatically (ECN) attained diameters ~17 nm had PdI, length, and crystallinity of 0.53, 514 nm & 70%, and 0.92, 1.0 μm & 48%, respectively.					
33422512	3	71	theme	functional	806:815	arg1	yields					729:734	The CNF yields	721:734	The CNF yields	721:734	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	71	theme	functional	806:815	arg1	properties					857:866	morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties	737:866	morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties	737:866	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	6	72	with	similar	1220:1226	arg1	time					1259:1262	optimal production time	1240:1262	optimal production time of 7.41 and 4.64 h	1240:1281	The HCN and ECN yields were similar (~20%) with optimal production time of 7.41 and 4.64 h, respectively.					
33422512	7	73	theme	stable	1348:1353	arg1	nanocolloids					1355:1366	thermally stable nanocolloids	1338:1366	thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1338:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	7	73	theme	stable	1348:1353	arg1	ECN					1313:1315	Both the HCN & ECN	1298:1315	Both the HCN & ECN	1298:1315	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	7	74	theme	~380 °C	1417:1423	arg1	temperatures					1401:1412	maximum thermal degradation temperatures	1373:1412	maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1373:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	3	75	dep	morphological	737:749	arg1	length					780:785	length	780:785	length	780:785	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	75	dep	morphological	737:749	arg1	index					767:771	polydispersity index	752:771	polydispersity index (PdI)	752:777	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	75	dep	morphological	737:749	arg1	diameter					791:798	diameter	791:798	diameter	791:798	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	3	75	dep	morphological	737:749	arg1	PdI					774:776	PdI	774:776	PdI	774:776	The CNF yields, morphological (polydispersity index (PdI), length and diameter), and functional (crystallinity and thermal degradation) properties were compared.					
33422512	7	76	theme	potential	1434:1442	arg1	~-16 mV					1444:1450	Zeta potential ~-16 mV	1429:1450	Zeta potential ~-16 mV	1429:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
33422512	0	77	theme	cellulose	62:70	arg1	properties					48:57	physical and functional properties	24:57	physical and functional properties of cellulose	24:70	Comparative analysis of physical and functional properties of cellulose nanofibers isolated from alkaline pre-treated wheat straw in optimized hydrochloric acid and enzymatic processes.					
33422512	1	78	theme	two-	342:345	arg1	stage-alkaline					347:360	stage-alkaline	347:360	stage-alkaline	347:360	Two methods, HCl and enzymatic treatments, were evaluated for diversification of morphological and functional properties of cellulose nanofibers (CNF) from two- stage-alkaline pre-treated wheat straw (WS).					
33422512	8	79	theme	production	1465:1474	arg1	methods					1476:1482	The two CNF production methods	1453:1482	The two CNF production methods	1453:1482	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	8	80	theme	CNF	1461:1463	arg1	methods					1476:1482	The two CNF production methods	1453:1482	The two CNF production methods	1453:1482	The two CNF production methods have potential synergetic effects on CNF production, morphological, and functional properties.					
33422512	7	81	theme	~-16 mV	1444:1450	arg1	temperatures					1401:1412	maximum thermal degradation temperatures	1373:1412	maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV	1373:1450	Both the HCN & ECN can be classified as thermally stable nanocolloids with maximum thermal degradation temperatures of ~380 °C and Zeta potential ~-16 mV.					
32946189	2	0	theme	synovial	336:343	arg1	fluid					345:349	synthetic synovial fluid	326:349	synthetic synovial fluid	326:349	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	1	1	theme	space	252:256	arg1	health					232:237	the health	228:237	the health of the joint space	228:256	Synovial fluid is dynamic in vivo with biological components changing in ratio and size depending on the health of the joint space, making it difficult to model in vitro.					
32946189	3	2	theme	reduced	863:869	arg1	production					883:892	reduced glutathione production	863:892	reduced glutathione production	863:892	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	3	theme	responses	1141:1149	arg1	responses					1141:1149	the 13 measured responses	1125:1149	the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface	1125:1283	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	3	theme	responses	1141:1149	arg1	12					1119:1120	12	1119:1120	12	1119:1120	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	5	4	theme	PL	1601:1602	arg1	ratios					1604:1609	PL ratios	1601:1609	three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions	1356:1634	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	2	5	with	interactions	405:416	arg1	surfaces					431:438	implant surfaces	423:438	implant surfaces	423:438	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	4	6	theme	single	983:988	arg1	variable					1005:1012	no single synovial fluid variable	980:1012	no single synovial fluid variable	980:1012	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	5	7	theme	inflamed	1414:1421	arg1	fluids					1423:1428	healthy, mild, or severely inflamed fluids	1387:1428	healthy, mild, or severely inflamed fluids	1387:1428	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	4	8	theme	significant	1156:1166	arg1	p < .05					1177:1183	p < .05	1177:1183	p < .05	1177:1183	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	8	theme	significant	1156:1166	arg1	effects					1168:1174	significant effects	1156:1174	significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface	1156:1283	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	9	theme	local	895:899	arg1	pH					910:911	local solution pH	895:911	local solution pH	895:911	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	10	theme	fluid	999:1003	arg1	variable					1005:1012	no single synovial fluid variable	980:1012	no single synovial fluid variable	980:1012	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	5	11	theme	mid-range	1496:1504	arg1	HA					1506:1507	mid-range HA	1496:1507	mid-range HA	1496:1507	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	4	12	theme	electrochemical	1230:1244	arg1	state					1257:1261	electrochemical capacitive state	1230:1261	electrochemical capacitive state	1230:1261	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	13	theme	individual	565:574	arg1	effects					576:582	the individual effects	561:582	the individual effects	561:582	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	14	dep	variables	631:639	arg1	concentrations					736:749	concentrations	736:749	concentrations	736:749	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	14	dep	variables	631:639	arg1	ratios					642:647	ratios	642:647	ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G)	642:729	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	6	15	with	combination	1674:1684	arg1	analysis					1699:1706	cluster analysis	1691:1706	cluster analysis	1691:1706	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	6	16	theme	Taguchi	1654:1660	arg1	approach					1662:1669	the Taguchi approach	1650:1669	the Taguchi approach	1650:1669	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	3	17	dep	acid	667:670	arg1	1					653:653	1	653:653	1	653:653	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	18	theme	fluid	613:617	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	18	theme	fluid	613:617	arg1	ions					830:833	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	ions	830:833	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	18	theme	fluid	613:617	arg1	Co2+					798:801	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	Co2+	798:801	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	5	19	theme	synovial	1462:1469	arg1	composition					1477:1487	the healthy synovial fluid composition	1450:1487	the healthy synovial fluid composition having mid-range HA	1450:1507	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	4	20	theme	macrophage	1201:1210	arg1	rate					1221:1224	the average macrophage survival rate	1189:1224	the average macrophage survival rate	1189:1224	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	21	theme	independent	592:602	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	21	theme	independent	592:602	arg1	ions					830:833	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	ions	830:833	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	21	theme	independent	592:602	arg1	Co2+					798:801	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	Co2+	798:801	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	22	theme	HA	1062:1063	arg1	ratio					1068:1072	HA:PL ratio	1062:1072	HA:PL ratio	1062:1072	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	23	theme	surface	1277:1283	arg1	rate					1221:1224	the average macrophage survival rate	1189:1224	the average macrophage survival rate	1189:1224	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	23	theme	surface	1277:1283	arg1	state					1257:1261	electrochemical capacitive state	1230:1261	electrochemical capacitive state	1230:1261	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	24	theme	hydrogen	758:765	arg1	H2					777:778	H2	777:778	H2	777:778	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	24	theme	hydrogen	758:765	arg1	peroxide					767:774	(3) hydrogen peroxide	754:774	(3) hydrogen peroxide (H2 O2 )	754:783	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	0	25	theme	cell-tribocorrosion	65:83	arg1	testing					85:91	in vitro cell-tribocorrosion testing	56:91	in vitro cell-tribocorrosion testing	56:91	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	4	26	with	responses	1141:1149	arg1	p < .05					1177:1183	p < .05	1177:1183	p < .05	1177:1183	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	26	with	responses	1141:1149	arg1	effects					1168:1174	significant effects	1156:1174	significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface	1156:1283	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	1	27	theme	biological	166:175	arg1	components					177:186	biological components	166:186	biological components changing in ratio and size depending on the health of the joint space	166:256	Synovial fluid is dynamic in vivo with biological components changing in ratio and size depending on the health of the joint space, making it difficult to model in vitro.					
32946189	0	28	theme	Taguchi	103:109	arg1	approach					117:124	the Taguchi array approach	99:124	the Taguchi array approach	99:124	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	29	theme	cobalt	790:795	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	29	theme	cobalt	790:795	arg1	Co2+					798:801	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	Co2+	798:801	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	30	theme	impact	1098:1103	arg1	Δ					1113:1113	Δ	1113:1113	Δ	1113:1113	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	30	theme	impact	1098:1103	arg1	factor					1105:1110	the largest impact factor	1086:1110	the largest impact factor (Δ)	1086:1114	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	6	31	theme	fluids	1790:1795	arg1	knowledge					1737:1745	our knowledge	1733:1745	our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface	1733:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	3	32	from	variables	631:639	arg1	production					883:892	reduced glutathione production	863:892	reduced glutathione production	863:892	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	32	from	variables	631:639	arg1	viability					849:857	macrophage viability	838:857	macrophage viability	838:857	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	2	33	theme	organic-tribological	384:403	arg1	interactions					405:416	single organic-tribological interactions	377:416	single organic-tribological interactions with implant surfaces	377:438	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	3	34	theme	electrochemical	948:962	arg1	response					964:971	the comprehensive CoCrMo alloy electrochemical response	917:971	the comprehensive CoCrMo alloy electrochemical response	917:971	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	6	35	theme	multivariate	1758:1769	arg1	fluids					1790:1795	complex multivariate synthetic synovial fluids	1750:1795	complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface	1750:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	5	36	theme	significant	1313:1323	arg1	responses					1325:1333	significant responses	1313:1333	significant responses from all trials	1313:1349	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	4	37	theme	PL	1065:1066	arg1	ratio					1068:1072	HA:PL ratio	1062:1072	HA:PL ratio	1062:1072	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	6	38	theme	synthetic	1771:1779	arg1	fluids					1790:1795	complex multivariate synthetic synovial fluids	1750:1795	complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface	1750:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	5	39	theme	Co2+	1527:1530	arg1	ions					1532:1535	no Co2+ ions	1524:1535	no Co2+ ions	1524:1535	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	0	40	theme	Synthetic	0:8	arg1	fluid					34:38	Synthetic periprosthetic synovial fluid	0:38	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing	0:91	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	41	theme	CoCrMo	935:940	arg1	response					964:971	the comprehensive CoCrMo alloy electrochemical response	917:971	the comprehensive CoCrMo alloy electrochemical response	917:971	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	6	42	theme	complex	1750:1756	arg1	fluids					1790:1795	complex multivariate synthetic synovial fluids	1750:1795	complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface	1750:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	0	43	theme	synovial	25:32	arg1	fluid					34:38	Synthetic periprosthetic synovial fluid	0:38	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing	0:91	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	5	44	with	ratios	1604:1609	arg1	H2					1616:1617	H2 O2	1616:1620	H2 O2	1616:1620	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	44	with	ratios	1604:1609	arg1	Co2+					1626:1629	Co2+	1626:1629	Co2+	1626:1629	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	45	contain	having	1489:1494	arg1	composition					1477:1487	the healthy synovial fluid composition	1450:1487	the healthy synovial fluid composition having mid-range HA	1450:1507	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	45	contain	having	1489:1494	arg2	HA					1506:1507	mid-range HA	1496:1507	mid-range HA	1496:1507	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	46	theme	PL	1509:1510	arg1	ratios					1512:1517	PL ratios	1509:1517	three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions	1356:1634	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	0	47	dep	in	56:57	arg1	vitro					59:63	vitro	59:63	vitro	59:63	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	48	theme	chromium	813:820	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	48	theme	chromium	813:820	arg1	ions					830:833	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	ions	830:833	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	2	49	theme	multiple	473:480	arg1	components					491:500	multiple solution components	473:500	multiple solution components	473:500	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	2	50	theme	synthetic	326:334	arg1	fluid					345:349	synthetic synovial fluid	326:349	synthetic synovial fluid	326:349	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	0	51	theme	in	56:57	arg1	testing					85:91	in vitro cell-tribocorrosion testing	56:91	in vitro cell-tribocorrosion testing	56:91	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	52	theme	acid	667:670	arg1	concentrations					736:749	concentrations	736:749	concentrations	736:749	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	52	theme	acid	667:670	arg1	ratios					642:647	ratios	642:647	ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G)	642:729	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	53	theme	Cr3+	823:826	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	53	theme	Cr3+	823:826	arg1	ions					830:833	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	ions	830:833	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	54	theme	orthogonal	519:528	arg1	array					530:534	a Taguchi orthogonal array	509:534	a Taguchi orthogonal array	509:534	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	1	55	theme	joint	246:250	arg1	space					252:256	the joint space	242:256	the joint space	242:256	Synovial fluid is dynamic in vivo with biological components changing in ratio and size depending on the health of the joint space, making it difficult to model in vitro.					
32946189	5	56	theme	low	1585:1587	arg1	HA					1598:1599	low and high HA	1585:1599	low and high HA	1585:1599	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	3	57	theme	macrophage	838:847	arg1	viability					849:857	macrophage viability	838:857	macrophage viability	838:857	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	58	theme	synovial	990:997	arg1	variable					1005:1012	no single synovial fluid variable	980:1012	no single synovial fluid variable	980:1012	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	59	theme	measured	1132:1139	arg1	responses					1141:1149	the 13 measured responses	1125:1149	the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface	1125:1283	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	5	60	theme	Cluster	1286:1292	arg1	analysis					1294:1301	Cluster analysis	1286:1301	Cluster analysis	1286:1301	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	61	dep	groups	1362:1367	arg1	corresponding					1370:1382	corresponding	1370:1382	corresponding	1370:1382	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	61	dep	groups	1362:1367	arg1	ratios					1512:1517	PL ratios	1509:1517	three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions	1356:1634	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	61	dep	groups	1362:1367	arg1	ratios					1604:1609	PL ratios	1601:1609	three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions	1356:1634	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	6	62	theme	cluster	1691:1697	arg1	analysis					1699:1706	cluster analysis	1691:1706	cluster analysis	1691:1706	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	5	63	theme	fluid	1471:1475	arg1	composition					1477:1487	the healthy synovial fluid composition	1450:1487	the healthy synovial fluid composition having mid-range HA	1450:1507	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	64	theme	inflamed	1555:1562	arg1	fluids					1564:1569	the severely inflamed fluids	1542:1569	the severely inflamed fluids consisting of low and high HA	1542:1599	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	2	65	theme	Previous	298:305	arg1	efforts					307:313	Previous efforts	298:313	Previous efforts to develop synthetic synovial fluid	298:349	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	3	66	theme	glutathione	871:881	arg1	production					883:892	reduced glutathione production	863:892	reduced glutathione production	863:892	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	67	theme	survival	1212:1219	arg1	rate					1221:1224	the average macrophage survival rate	1189:1224	the average macrophage survival rate	1189:1224	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	5	68	theme	high	1593:1596	arg1	HA					1598:1599	low and high HA	1585:1599	low and high HA	1585:1599	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	69	with	ratios	1512:1517	arg1	fluids					1564:1569	the severely inflamed fluids	1542:1569	the severely inflamed fluids consisting of low and high HA	1542:1599	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	69	with	ratios	1512:1517	arg1	ions					1532:1535	no Co2+ ions	1524:1535	no Co2+ ions	1524:1535	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	3	70	theme	albumin	705:711	arg1	concentrations					736:749	concentrations	736:749	concentrations	736:749	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	70	theme	albumin	705:711	arg1	ratios					642:647	ratios	642:647	ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G)	642:729	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	71	theme	synovial	604:611	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	71	theme	synovial	604:611	arg1	ions					830:833	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	ions	830:833	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	71	theme	synovial	604:611	arg1	Co2+					798:801	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	Co2+	798:801	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	5	72	from	trials	1344:1349	arg1	responses					1325:1333	significant responses	1313:1333	significant responses from all trials	1313:1349	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	5	73	theme	healthy	1454:1460	arg1	composition					1477:1487	the healthy synovial fluid composition	1450:1487	the healthy synovial fluid composition having mid-range HA	1450:1507	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	1	74	theme	Synovial	127:134	arg1	fluid					136:140	Synovial fluid	127:140	Synovial fluid	127:140	Synovial fluid is dynamic in vivo with biological components changing in ratio and size depending on the health of the joint space, making it difficult to model in vitro.					
32946189	4	75	theme	average	1193:1199	arg1	rate					1221:1224	the average macrophage survival rate	1189:1224	the average macrophage survival rate	1189:1224	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	76	theme	CoCrMo	1270:1275	arg1	surface					1277:1283	the CoCrMo surface	1266:1283	the CoCrMo surface	1266:1283	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	77	theme	composition	619:629	arg1	variables					631:639	five independent synovial fluid composition variables	587:639	five independent synovial fluid composition variables	587:639	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	77	theme	composition	619:629	arg1	ions					830:833	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	ions	830:833	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	77	theme	composition	619:629	arg1	Co2+					798:801	(4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions	786:833	Co2+	798:801	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	78	theme	peroxide	767:774	arg1	concentrations					736:749	concentrations	736:749	concentrations	736:749	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	78	theme	peroxide	767:774	arg1	ratios					642:647	ratios	642:647	ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G)	642:729	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	79	theme	collective	1041:1050	arg1	response					1052:1059	the collective response	1037:1059	the collective response	1037:1059	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	80	dep	albumin	705:711	arg1	2					702:702	2	702:702	2	702:702	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	81	theme	largest	1090:1096	arg1	Δ					1113:1113	Δ	1113:1113	Δ	1113:1113	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	81	theme	largest	1090:1096	arg1	factor					1105:1110	the largest impact factor	1086:1110	the largest impact factor (Δ)	1086:1114	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	82	theme	capacitive	1246:1255	arg1	state					1257:1261	electrochemical capacitive state	1230:1261	electrochemical capacitive state	1230:1261	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	0	83	theme	array	111:115	arg1	approach					117:124	the Taguchi array approach	99:124	the Taguchi array approach	99:124	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	84	theme	solution	901:908	arg1	pH					910:911	local solution pH	895:911	local solution pH	895:911	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	85	dep	peroxide	767:774	arg1	3					755:755	3	755:755	3	755:755	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	4	86	from	effects	1168:1174	arg1	rate					1221:1224	the average macrophage survival rate	1189:1224	the average macrophage survival rate	1189:1224	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	4	86	from	effects	1168:1174	arg1	state					1257:1261	electrochemical capacitive state	1230:1261	electrochemical capacitive state	1230:1261	While no single synovial fluid variable significantly affected the collective response, HA:PL ratio resulted in the largest impact factor (Δ) on 12 of the 13 measured responses with significant effects (p < .05) on the average macrophage survival rate and electrochemical capacitive state of the CoCrMo surface.					
32946189	3	87	dep	A	726:726	arg1	G					728:728	G	728:728	G	728:728	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	88	theme	alloy	942:946	arg1	response					964:971	the comprehensive CoCrMo alloy electrochemical response	917:971	the comprehensive CoCrMo alloy electrochemical response	917:971	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	6	89	theme	synovial	1781:1788	arg1	fluids					1790:1795	complex multivariate synthetic synovial fluids	1750:1795	complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface	1750:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	2	90	theme	single	377:382	arg1	interactions					405:416	single organic-tribological interactions	377:416	single organic-tribological interactions with implant surfaces	377:438	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	0	91	theme	periprosthetic	10:23	arg1	fluid					34:38	Synthetic periprosthetic synovial fluid	0:38	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing	0:91	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	92	theme	comprehensive	921:933	arg1	response					964:971	the comprehensive CoCrMo alloy electrochemical response	917:971	the comprehensive CoCrMo alloy electrochemical response	917:971	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	2	93	dep	efforts	307:313	arg1	develop					318:324	develop	318:324	to develop synthetic synovial fluid	315:349	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	0	94	theme	fluid	34:38	arg1	development					40:50	Synthetic periprosthetic synovial fluid development	0:50	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing	0:91	Synthetic periprosthetic synovial fluid development for in vitro cell-tribocorrosion testing using the Taguchi array approach.					
32946189	3	95	dep	HA	690:691	arg1	PL					693:694	PL	693:694	PL	693:694	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	6	96	dep	fluids	1790:1795	arg1	influence					1797:1805	influence	1797:1805	influence on macrophage and electrochemical behavior at the cell-solution-metal interface	1797:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	5	97	theme	healthy	1387:1393	arg1	fluids					1423:1428	healthy, mild, or severely inflamed fluids	1387:1428	healthy, mild, or severely inflamed fluids	1387:1428	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	2	98	theme	implant	423:429	arg1	surfaces					431:438	implant surfaces	423:438	implant surfaces	423:438	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	5	99	dep	H2	1616:1617	arg1	ions					1631:1634	ions	1631:1634	ions	1631:1634	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	2	100	theme	solution	482:489	arg1	components					491:500	multiple solution components	473:500	multiple solution components	473:500	Previous efforts to develop synthetic synovial fluid have typically focused on single organic-tribological interactions with implant surfaces, thus ignoring interplay between multiple solution components.					
32946189	5	101	theme	mild	1396:1399	arg1	fluids					1423:1428	healthy, mild, or severely inflamed fluids	1387:1428	healthy, mild, or severely inflamed fluids	1387:1428	Cluster analysis separated significant responses from all trials into three groups, corresponding to healthy, mild, or severely inflamed fluids, respectively; with the healthy synovial fluid composition having mid-range HA:PL ratios with no Co2+ ions, and the severely inflamed fluids consisting of low and high HA:PL ratios with H2 O2 and Co2+ ions.					
32946189	6	102	theme	cell-solution-metal	1857:1875	arg1	interface					1877:1885	the cell-solution-metal interface	1853:1885	the cell-solution-metal interface	1853:1885	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
32946189	1	103	with	dynamic	145:151	arg1	components					177:186	biological components	166:186	biological components changing in ratio and size depending on the health of the joint space	166:256	Synovial fluid is dynamic in vivo with biological components changing in ratio and size depending on the health of the joint space, making it difficult to model in vitro.					
32946189	3	104	theme	Taguchi	511:517	arg1	array					530:534	a Taguchi orthogonal array	509:534	a Taguchi orthogonal array	509:534	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	105	theme	hyaluronic	656:665	arg1	acid					667:670	(1) hyaluronic acid to phospholipids	652:687	(1) hyaluronic acid to phospholipids (HA:PL)	652:695	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	3	105	theme	hyaluronic	656:665	arg1	HA					690:691	HA	690:691	HA	690:691	Using a Taguchi orthogonal array, we were able to isolate the individual effects of five independent synovial fluid composition variables: ratios of (1) hyaluronic acid to phospholipids (HA:PL) and (2) albumin to globulin (A:G), and concentrations of (3) hydrogen peroxide (H2 O2 ), (4) cobalt (Co2+ ) and (5) chromium (Cr3+ ) ions on macrophage viability and reduced glutathione production, local solution pH and the comprehensive CoCrMo alloy electrochemical response.					
32946189	6	106	theme	electrochemical	1825:1839	arg1	behavior					1841:1848	electrochemical behavior	1825:1848	electrochemical behavior	1825:1848	By utilizing the Taguchi approach in combination with cluster analysis, we were able to advance our knowledge of complex multivariate synthetic synovial fluids influence on macrophage and electrochemical behavior at the cell-solution-metal interface.					
33803082	0	0	theme	Theobroma	74:82	arg1	L.					90:91	Theobroma cacao L.	74:91	Theobroma cacao L.	74:91	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	0	theme	Theobroma	74:82	arg1	Husks					67:71	Cocoa Pod Husks	57:71	Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks	57:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	5	1	theme	galacturonic	879:890	arg1	pectin					903:908	52.06 g galacturonic acid/100 g pectin	871:908	52.06 g galacturonic acid/100 g pectin	871:908	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	6	2	theme	pectin/100	1021:1030	arg1	feedstock					1034:1042	8.08 g pectin/100 g feedstock	1014:1042	8.08 g pectin/100 g feedstock	1014:1042	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	7	3	theme	g	1171:1171	arg1	feedstock					1173:1181	8.28 g/100 g feedstock	1160:1181	8.28 g/100 g feedstock	1160:1181	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	2	4	theme	composite	321:329	arg1	CCD					339:341	CCD	339:341	CCD	339:341	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	2	4	theme	composite	321:329	arg1	design					331:336	a central composite design	311:336	a central composite design (CCD)	311:342	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	7	5	theme	feedstock	1173:1181	arg1	yield					1151:1155	a pectin yield	1142:1155	a pectin yield of 8.28 g/100 g feedstock	1142:1181	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	7	5	theme	feedstock	1173:1181	arg1	content					1207:1213	a galacturonic acid content	1187:1213	a galacturonic acid content of 42.77 g/100 g pectin	1187:1237	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	5	6	theme	acid/100	943:950	arg1	feedstock					954:962	galacturonic acid/100 g feedstock	930:962	a yield 5.31 g galacturonic acid/100 g feedstock	915:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	7	theme	acid/100	892:899	arg1	pectin					903:908	52.06 g galacturonic acid/100 g pectin	871:908	52.06 g galacturonic acid/100 g pectin	871:908	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	6	8	theme	g	1019:1019	arg1	feedstock					1034:1042	8.08 g pectin/100 g feedstock	1014:1042	8.08 g pectin/100 g feedstock	1014:1042	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	2	9	theme	central	313:319	arg1	CCD					339:341	CCD	339:341	CCD	339:341	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	2	9	theme	central	313:319	arg1	design					331:336	a central composite design	311:336	a central composite design (CCD)	311:342	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	8	10	theme	husks	1431:1435	arg1	valorization					1409:1420	the valorization	1405:1420	the valorization of cocoa husks	1405:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	5	11	theme	g	952:952	arg1	feedstock					954:962	galacturonic acid/100 g feedstock	930:962	a yield 5.31 g galacturonic acid/100 g feedstock	915:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	12	theme	g	901:901	arg1	pectin					903:908	52.06 g galacturonic acid/100 g pectin	871:908	52.06 g galacturonic acid/100 g pectin	871:908	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	4	13	theme	=	686:686	arg1	95.35					688:692	R2 = 95.35	683:692	R2 = 95.35	683:692	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	13	theme	=	686:686	arg1	yield					656:660	galacturonic acid yield	638:660	galacturonic acid yield (g/100 g feedstock) (R2 = 95.35)	638:693	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	8	14	theme	cocoa	1425:1429	arg1	husks					1431:1435	cocoa husks	1425:1435	cocoa husks	1425:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	1	15	theme	enzymatic	219:227	arg1	extraction					229:238	the enzymatic extraction	215:238	the enzymatic extraction of pectin using cellulases	215:265	We aimed to explore the enzymatic extraction of pectin using cellulases.					
33803082	0	16	theme	cacao	84:88	arg1	L.					90:91	Theobroma cacao L.	74:91	Theobroma cacao L.	74:91	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	16	theme	cacao	84:88	arg1	Husks					67:71	Cocoa Pod Husks	57:71	Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks	57:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	5	17	theme	g	858:858	arg1	pectin					903:908	52.06 g galacturonic acid/100 g pectin	871:908	52.06 g galacturonic acid/100 g pectin	871:908	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	17	theme	g	858:858	arg1	yield					917:921	a yield	915:921	a yield 5.31 g galacturonic acid/100 g feedstock	915:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	17	theme	g	858:858	arg1	yield					837:841	a pectin yield	828:841	a pectin yield of 10.20 g/100 g feedstock	828:868	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	4	18	theme	R2	683:684	arg1	95.35					688:692	R2 = 95.35	683:692	R2 = 95.35	683:692	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	18	theme	R2	683:684	arg1	yield					656:660	galacturonic acid yield	638:660	galacturonic acid yield (g/100 g feedstock) (R2 = 95.35)	638:693	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	5	19	theme	µL	757:758	arg1	g-1					760:762	40 µL g-1	754:762	40 µL g-1 of enzyme	754:772	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	20	theme	6.0	724:726	arg1	%					727:727	%	727:727	%	727:727	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	6	21	theme	extraction	984:993	arg1	method					995:1000	the chemical extraction method	971:1000	the chemical extraction method	971:1000	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	4	22	theme	pectin	531:536	arg1	97.02					569:573	97.02	569:573	97.02	569:573	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	22	theme	pectin	531:536	arg1	husk					557:560	g/100 g dry husk	545:560	g/100 g dry husk	545:560	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	22	theme	pectin	531:536	arg1	yield					538:542	pectin yield	531:542	pectin yield (g/100 g dry husk) (R2 = 97.02)	531:574	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	7	23	theme	pectin	1144:1149	arg1	yield					1151:1155	a pectin yield	1142:1155	a pectin yield of 8.28 g/100 g feedstock	1142:1181	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	4	24	theme	dry	553:555	arg1	husk					557:560	g/100 g dry husk	545:560	g/100 g dry husk	545:560	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	24	theme	dry	553:555	arg1	yield					538:542	pectin yield	531:542	pectin yield (g/100 g dry husk) (R2 = 97.02)	531:574	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	7	25	dep	g	1230:1230	arg1	pectin					1232:1237	pectin	1232:1237	42.77 g/100 g pectin	1218:1237	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	8	26	theme	typical	1330:1336	arg1	features					1321:1328	rheological and physicochemical features	1289:1328	rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks	1289:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	4	27	theme	g	551:551	arg1	husk					557:560	g/100 g dry husk	545:560	g/100 g dry husk	545:560	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	27	theme	g	551:551	arg1	yield					538:542	pectin yield	531:542	pectin yield (g/100 g dry husk) (R2 = 97.02)	531:574	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	5	28	theme	52.06	871:875	arg1	g					877:877	g	877:877	g	877:877	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	0	29	theme	beans	188:192	arg1	processing					168:177	the processing	164:177	the processing of cocoa beans	164:192	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	5	30	theme	%	727:727	arg1	concentration					739:751	6.0% feedstock concentration	724:751	6.0% feedstock concentration	724:751	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	30	theme	%	727:727	arg1	parameters					708:717	The optimal parameters	696:717	The optimal parameters	696:717	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	0	31	theme	Enzymatic	0:8	arg1	waste					141:145	a waste	139:145	a waste generated during the processing of cocoa beans	139:192	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	31	theme	Enzymatic	0:8	arg1	Characterization					25:40	Characterization	25:40	Characterization	25:40	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	31	theme	Enzymatic	0:8	arg1	Extraction					10:19	Enzymatic Extraction	0:19	Enzymatic Extraction	0:19	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	8	32	theme	optimized	1268:1276	arg1	pectin					1278:1283	Enzymatically optimized pectin	1254:1283	Enzymatically optimized pectin	1254:1283	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	0	33	theme	cocoa	182:186	arg1	beans					188:192	cocoa beans	182:192	cocoa beans	182:192	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	2	34	theme	extraction	272:281	arg1	process					283:289	The extraction process	268:289	The extraction process	268:289	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	7	35	theme	8.28	1160:1163	arg1	feedstock					1173:1181	8.28 g/100 g feedstock	1160:1181	8.28 g/100 g feedstock	1160:1181	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	1	36	theme	pectin	243:248	arg1	cellulases					256:265	pectin using cellulases	243:265	pectin using cellulases	243:265	We aimed to explore the enzymatic extraction of pectin using cellulases.					
33803082	4	37	theme	g	610:610	arg1	content					595:601	galacturonic acid content	577:601	galacturonic acid content (g/100 g pectin) (R2 = 96.90)	577:631	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	37	theme	g	610:610	arg1	pectin					612:617	g/100 g pectin	604:617	g/100 g pectin	604:617	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	5	38	dep	g	928:928	arg1	feedstock					954:962	galacturonic acid/100 g feedstock	930:962	a yield 5.31 g galacturonic acid/100 g feedstock	915:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	39	theme	feedstock	729:737	arg1	concentration					739:751	6.0% feedstock concentration	724:751	6.0% feedstock concentration	724:751	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	39	theme	feedstock	729:737	arg1	parameters					708:717	The optimal parameters	696:717	The optimal parameters	696:717	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	1	40	theme	using	250:254	arg1	cellulases					256:265	pectin using cellulases	243:265	pectin using cellulases	243:265	We aimed to explore the enzymatic extraction of pectin using cellulases.					
33803082	0	41	theme	Celluclast®	100:110	arg1	husks					129:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	2	42	theme	surface	369:375	arg1	RSM					390:392	RSM	390:392	RSM	390:392	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	2	42	theme	surface	369:375	arg1	methodology					377:387	response surface methodology	360:387	response surface methodology (RSM)	360:393	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	0	43	theme	Pectin	45:50	arg1	waste					141:145	a waste	139:145	a waste generated during the processing of cocoa beans	139:192	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	43	theme	Pectin	45:50	arg1	Characterization					25:40	Characterization	25:40	Characterization	25:40	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	43	theme	Pectin	45:50	arg1	Extraction					10:19	Enzymatic Extraction	0:19	Enzymatic Extraction	0:19	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	1	44	theme	cellulases	256:265	arg1	extraction					229:238	the enzymatic extraction	215:238	the enzymatic extraction of pectin using cellulases	215:265	We aimed to explore the enzymatic extraction of pectin using cellulases.					
33803082	0	45	theme	Cocoa	119:123	arg1	husks					129:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	4	46	theme	galacturonic	638:649	arg1	95.35					688:692	R2 = 95.35	683:692	R2 = 95.35	683:692	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	46	theme	galacturonic	638:649	arg1	feedstock					671:679	g/100 g feedstock	663:679	g/100 g feedstock	663:679	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	46	theme	galacturonic	638:649	arg1	yield					656:660	galacturonic acid yield	638:660	galacturonic acid yield (g/100 g feedstock) (R2 = 95.35)	638:693	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	7	47	theme	g	1230:1230	arg1	yield					1151:1155	a pectin yield	1142:1155	a pectin yield of 8.28 g/100 g feedstock	1142:1181	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	7	47	theme	g	1230:1230	arg1	content					1207:1213	a galacturonic acid content	1187:1213	a galacturonic acid content of 42.77 g/100 g pectin	1187:1237	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	2	48	theme	response	360:367	arg1	RSM					390:392	RSM	390:392	RSM	390:392	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	2	48	theme	response	360:367	arg1	methodology					377:387	response surface methodology	360:387	response surface methodology (RSM)	360:393	The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM).					
33803082	0	49	theme	L.	116:117	arg1	husks					129:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	50	from	Husks	67:71	arg1	waste					141:145	a waste	139:145	a waste generated during the processing of cocoa beans	139:192	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	50	from	Husks	67:71	arg1	Characterization					25:40	Characterization	25:40	Characterization	25:40	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	50	from	Husks	67:71	arg1	Extraction					10:19	Enzymatic Extraction	0:19	Enzymatic Extraction	0:19	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	6	51	theme	chemical	975:982	arg1	method					995:1000	the chemical extraction method	971:1000	the chemical extraction method	971:1000	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	5	52	theme	galacturonic	930:941	arg1	feedstock					954:962	galacturonic acid/100 g feedstock	930:962	a yield 5.31 g galacturonic acid/100 g feedstock	915:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	0	53	theme	Cocoa	57:61	arg1	L.					90:91	Theobroma cacao L.	74:91	Theobroma cacao L.	74:91	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	53	theme	Cocoa	57:61	arg1	Husks					67:71	Cocoa Pod Husks	57:71	Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks	57:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	8	54	theme	rheological	1289:1299	arg1	features					1321:1328	rheological and physicochemical features	1289:1328	rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks	1289:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	6	55	theme	acid	1063:1066	arg1	content					1068:1074	a galacturonic acid content	1048:1074	a galacturonic acid content of 60.97 g/100 g pectin	1048:1098	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	4	56	theme	g	669:669	arg1	feedstock					671:679	g/100 g feedstock	663:679	g/100 g feedstock	663:679	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	56	theme	g	669:669	arg1	yield					656:660	galacturonic acid yield	638:660	galacturonic acid yield (g/100 g feedstock) (R2 = 95.35)	638:693	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	5	57	theme	optimal	700:706	arg1	concentration					739:751	6.0% feedstock concentration	724:751	6.0% feedstock concentration	724:751	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	57	theme	optimal	700:706	arg1	parameters					708:717	The optimal parameters	696:717	The optimal parameters	696:717	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	6	58	theme	galacturonic	1050:1061	arg1	content					1068:1074	a galacturonic acid content	1048:1074	a galacturonic acid content of 60.97 g/100 g pectin	1048:1098	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	5	59	dep	g	858:858	arg1	feedstock					860:868	feedstock	860:868	10.20 g/100 g feedstock	846:868	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	0	60	theme	pod	125:127	arg1	husks					129:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Celluclast® 1.5 L. Cocoa pod husks	100:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	4	61	theme	dependent	497:505	arg1	variables					507:515	Three dependent variables	491:515	Three dependent variables	491:515	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	62	theme	acid	651:654	arg1	95.35					688:692	R2 = 95.35	683:692	R2 = 95.35	683:692	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	62	theme	acid	651:654	arg1	feedstock					671:679	g/100 g feedstock	663:679	g/100 g feedstock	663:679	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	62	theme	acid	651:654	arg1	yield					656:660	galacturonic acid yield	638:660	galacturonic acid yield (g/100 g feedstock) (R2 = 95.35)	638:693	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	7	63	theme	galacturonic	1189:1200	arg1	content					1207:1213	a galacturonic acid content	1187:1213	a galacturonic acid content of 42.77 g/100 g pectin	1187:1237	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	8	64	theme	physicochemical	1305:1319	arg1	features					1321:1328	rheological and physicochemical features	1289:1328	rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks	1289:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	6	65	theme	feedstock	1034:1042	arg1	yield					1005:1009	a yield	1003:1009	a yield of 8.08 g pectin/100 g feedstock	1003:1042	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	6	65	theme	feedstock	1034:1042	arg1	content					1068:1074	a galacturonic acid content	1048:1074	a galacturonic acid content of 60.97 g/100 g pectin	1048:1098	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	4	66	dep	studied	522:528	arg1	yield					538:542	pectin yield	531:542	pectin yield (g/100 g dry husk) (R2 = 97.02)	531:574	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	pectin					612:617	g/100 g pectin	604:617	g/100 g pectin	604:617	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	95.35					688:692	R2 = 95.35	683:692	R2 = 95.35	683:692	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	feedstock					671:679	g/100 g feedstock	663:679	g/100 g feedstock	663:679	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	content					595:601	galacturonic acid content	577:601	galacturonic acid content (g/100 g pectin) (R2 = 96.90)	577:631	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	97.02					569:573	97.02	569:573	97.02	569:573	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	yield					656:660	galacturonic acid yield	638:660	galacturonic acid yield (g/100 g feedstock) (R2 = 95.35)	638:693	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	husk					557:560	g/100 g dry husk	545:560	g/100 g dry husk	545:560	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	66	dep	studied	522:528	arg1	96.90					626:630	96.90	626:630	96.90	626:630	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	5	67	theme	enzyme	767:772	arg1	concentration					739:751	6.0% feedstock concentration	724:751	6.0% feedstock concentration	724:751	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	67	theme	enzyme	767:772	arg1	conditions					788:797	conditions	788:797	conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock	788:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	67	theme	enzyme	767:772	arg1	g-1					760:762	40 µL g-1	754:762	40 µL g-1 of enzyme	754:772	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	67	theme	enzyme	767:772	arg1	h					785:785	18.54 h	779:785	18.54 h	779:785	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	67	theme	enzyme	767:772	arg1	parameters					708:717	The optimal parameters	696:717	The optimal parameters	696:717	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	3	68	theme	feedstock	426:434	arg1	parameters					400:409	The parameters	396:409	The parameters optimized	396:419	The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h).					
33803082	3	68	theme	feedstock	426:434	arg1	%					451:451	%	451:451	%	451:451	The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h).					
33803082	3	68	theme	feedstock	426:434	arg1	concentration					436:448	feedstock concentration	426:448	feedstock concentration (%)	426:452	The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h).					
33803082	7	69	theme	assisted	1121:1128	arg1	sonication					1130:1139	assisted sonication	1121:1139	assisted sonication	1121:1139	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	7	70	theme	acid	1202:1205	arg1	content					1207:1213	a galacturonic acid content	1187:1213	a galacturonic acid content of 42.77 g/100 g pectin	1187:1237	Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained.					
33803082	6	71	theme	g	1032:1032	arg1	feedstock					1034:1042	8.08 g pectin/100 g feedstock	1014:1042	8.08 g pectin/100 g feedstock	1014:1042	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	4	72	theme	galacturonic	577:588	arg1	content					595:601	galacturonic acid content	577:601	galacturonic acid content (g/100 g pectin) (R2 = 96.90)	577:631	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	72	theme	galacturonic	577:588	arg1	96.90					626:630	96.90	626:630	96.90	626:630	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	72	theme	galacturonic	577:588	arg1	pectin					612:617	g/100 g pectin	604:617	g/100 g pectin	604:617	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	73	theme	acid	590:593	arg1	content					595:601	galacturonic acid content	577:601	galacturonic acid content (g/100 g pectin) (R2 = 96.90)	577:631	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	73	theme	acid	590:593	arg1	96.90					626:630	96.90	626:630	96.90	626:630	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	4	73	theme	acid	590:593	arg1	pectin					612:617	g/100 g pectin	604:617	g/100 g pectin	604:617	Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35).					
33803082	5	74	dep	yield	917:921	arg1	g					928:928	5.31 g	923:928	a yield 5.31 g galacturonic acid/100 g feedstock	915:962	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	5	75	theme	pectin	830:835	arg1	yield					837:841	a pectin yield	828:841	a pectin yield of 10.20 g/100 g feedstock	828:868	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	8	76	contain	has	1285:1287	arg1	pectin					1278:1283	Enzymatically optimized pectin	1254:1283	Enzymatically optimized pectin	1254:1283	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	8	76	contain	has	1285:1287	arg2	features					1321:1328	rheological and physicochemical features	1289:1328	rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks	1289:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	3	77	theme	enzyme	455:460	arg1	µL/g					470:473	µL/g	470:473	µL/g	470:473	The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h).					
33803082	3	77	theme	enzyme	455:460	arg1	dosage					462:467	enzyme dosage	455:467	enzyme dosage (µL/g)	455:474	The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h).					
33803082	8	78	theme	interesting	1377:1387	arg1	alternative					1389:1399	an interesting alternative	1374:1399	an interesting alternative for the valorization of cocoa husks	1374:1435	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	8	79	theme	biomaterial	1346:1356	arg1	typical					1330:1336	typical	1330:1336	typical	1330:1336	Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.					
33803082	0	80	theme	Pod	63:65	arg1	L.					90:91	Theobroma cacao L.	74:91	Theobroma cacao L.	74:91	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	0	80	theme	Pod	63:65	arg1	Husks					67:71	Cocoa Pod Husks	57:71	Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks	57:133	Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Cocoa pod husks are a waste generated during the processing of cocoa beans.					
33803082	5	81	theme	g	877:877	arg1	pectin					903:908	52.06 g galacturonic acid/100 g pectin	871:908	52.06 g galacturonic acid/100 g pectin	871:908	The optimal parameters were 6.0% feedstock concentration, 40 µL g-1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock.					
33803082	6	82	dep	g	1091:1091	arg1	pectin					1093:1098	pectin	1093:1098	60.97 g/100 g pectin	1079:1098	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	6	83	theme	g	1091:1091	arg1	yield					1005:1009	a yield	1003:1009	a yield of 8.08 g pectin/100 g feedstock	1003:1042	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33803082	6	83	theme	g	1091:1091	arg1	content					1068:1074	a galacturonic acid content	1048:1074	a galacturonic acid content of 60.97 g/100 g pectin	1048:1098	Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained.					
33934170	1	0	theme	nutrients	313:321	arg1	nutrients					313:321	nutrients	313:321	nutrients	313:321	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	0	theme	nutrients	313:321	arg1	compounds					337:345	bioactive compounds	327:345	bioactive compounds	327:345	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	0	theme	nutrients	313:321	arg1	variety					302:308	a variety	300:308	a variety of nutrients and bioactive compounds	300:345	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	2	1	theme	human	683:687	arg1	inocula					695:701	a pooled human fecal inocula	674:701	a pooled human fecal inocula	674:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	0	2	theme	Prebiotic	141:149	arg1	Properties					151:160	Prebiotic Properties	141:160	Prebiotic Properties	141:160	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	6	3	attach	linked	1231:1236	arg1	presence					1276:1283	the presence	1272:1283	the presence of fructans and different phenolic compounds found in FCAB	1272:1342	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	6	3	attach	linked	1231:1236	arg1	contents					1241:1248	contents	1241:1248	contents of dietary fibers	1241:1266	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	6	3	attach	linked	1231:1236	arg2	effects					1214:1220	These effects	1208:1220	These effects	1208:1220	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	5	4	theme	colonic	1180:1186	arg1	media					1201:1205	colonic fermentation media	1180:1205	colonic fermentation media	1180:1205	Furthermore, FCAB decreased pH and increased the production of short-chain fatty acids in colonic fermentation media.					
33934170	4	5	theme	Enterobacteriaceae	1042:1059	arg1	counts					1032:1037	the counts	1028:1037	the counts of Enterobacteriaceae during colonic fermentation	1028:1087	FCAB increased the counts of lactic acid bacteria and decreased the counts of Enterobacteriaceae during colonic fermentation.					
33934170	3	6	theme	Bacteroides/Prevotella	867:888	arg1	abundance					854:862	the relative abundance	841:862	the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum	841:961	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	2	7	theme	microbial	517:525	arg1	groups					527:532	distinct microbial groups	508:532	distinct microbial groups found as part of human colonic microbiota	508:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	0	8	from	Impact	0:5	arg1	Composition					59:69	Composition	59:69	Composition	59:69	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	0	8	from	Impact	0:5	arg1	Activity					85:92	Metabolic Activity	75:92	Metabolic Activity	75:92	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	7	9	dep	induced	1376:1382	arg1	indicate					1487:1494	indicate	1487:1494	indicate prebiotic properties	1487:1515	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	7	10	theme	microbiota	1460:1469	arg1	composition					1408:1418	composition	1408:1418	composition	1408:1418	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	7	10	theme	microbiota	1460:1469	arg1	activity					1434:1441	metabolic activity	1424:1441	metabolic activity	1424:1441	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	0	11	theme	Metabolic	75:83	arg1	Activity					85:92	Metabolic Activity	75:92	Metabolic Activity	75:92	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	1	12	theme	compounds	337:345	arg1	nutrients					313:321	nutrients	313:321	nutrients	313:321	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	12	theme	compounds	337:345	arg1	compounds					337:345	bioactive compounds	327:345	bioactive compounds	327:345	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	12	theme	compounds	337:345	arg1	variety					302:308	a variety	300:308	a variety of nutrients and bioactive compounds	300:345	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	6	13	theme	compounds	1320:1328	arg1	contents					1241:1248	contents	1241:1248	contents of dietary fibers	1241:1266	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	6	13	theme	compounds	1320:1328	arg1	presence					1276:1283	the presence	1272:1283	the presence of fructans and different phenolic compounds found in FCAB	1272:1342	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	2	14	theme	acid	616:619	arg1	production					621:630	short-chain fatty acid production	598:630	short-chain fatty acid production	598:630	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	7	15	theme	prebiotic	1496:1504	arg1	properties					1506:1515	prebiotic properties	1496:1515	prebiotic properties	1496:1515	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	7	16	from	alterations	1393:1403	arg1	composition					1408:1418	composition	1408:1418	composition	1408:1418	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	7	16	from	alterations	1393:1403	arg1	activity					1434:1441	metabolic activity	1424:1441	metabolic activity	1424:1441	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	0	17	theme	Human	97:101	arg1	Microbiota					111:120	Human Colonic Microbiota	97:120	Human Colonic Microbiota	97:120	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	2	18	theme	short-chain	598:608	arg1	production					621:630	short-chain fatty acid production	598:630	short-chain fatty acid production	598:630	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	19	theme	fatty	610:614	arg1	production					621:630	short-chain fatty acid production	598:630	short-chain fatty acid production	598:630	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	1	20	theme	Cashew	163:168	arg1	by-product					176:185	Cashew apple by-product	163:185	Cashew apple by-product (CAB)	163:191	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	20	theme	Cashew	163:168	arg1	CAB					188:190	CAB	188:190	CAB	188:190	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	2	21	theme	simulated	426:434	arg1	digestion					453:461	a simulated gastrointestinal digestion	424:461	a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula	424:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	5	22	theme	short-chain	1153:1163	arg1	acids					1171:1175	short-chain fatty acids	1153:1175	short-chain fatty acids	1153:1175	Furthermore, FCAB decreased pH and increased the production of short-chain fatty acids in colonic fermentation media.					
33934170	6	23	theme	different	1301:1309	arg1	compounds					1320:1328	different phenolic compounds	1301:1328	different phenolic compounds	1301:1328	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	3	24	theme	coccoides	923:931	arg1	abundance					854:862	the relative abundance	841:862	the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum	841:961	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	1	25	theme	apple	170:174	arg1	by-product					176:185	Cashew apple by-product	163:185	Cashew apple by-product (CAB)	163:191	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	25	theme	apple	170:174	arg1	CAB					188:190	CAB	188:190	CAB	188:190	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	4	26	theme	colonic	1068:1074	arg1	fermentation					1076:1087	colonic fermentation	1068:1087	colonic fermentation	1068:1087	FCAB increased the counts of lactic acid bacteria and decreased the counts of Enterobacteriaceae during colonic fermentation.					
33934170	5	27	theme	fatty	1165:1169	arg1	acids					1171:1175	short-chain fatty acids	1153:1175	short-chain fatty acids	1153:1175	Furthermore, FCAB decreased pH and increased the production of short-chain fatty acids in colonic fermentation media.					
33934170	2	28	theme	in	646:647	arg1	fermentation					655:666	a 24-h in vitro fermentation	639:666	a 24-h in vitro fermentation using a pooled human fecal inocula	639:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	6	29	theme	fructans	1288:1295	arg1	contents					1241:1248	contents	1241:1248	contents of dietary fibers	1241:1266	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	6	29	theme	fructans	1288:1295	arg1	presence					1276:1283	the presence	1272:1283	the presence of fructans and different phenolic compounds found in FCAB	1272:1342	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	2	30	theme	24-h	641:644	arg1	fermentation					655:666	a 24-h in vitro fermentation	639:666	a 24-h in vitro fermentation using a pooled human fecal inocula	639:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	6	31	theme	phenolic	1311:1318	arg1	compounds					1320:1328	different phenolic compounds	1301:1328	different phenolic compounds	1301:1328	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	5	32	from	production	1139:1148	arg1	media					1201:1205	colonic fermentation media	1180:1205	colonic fermentation media	1180:1205	Furthermore, FCAB decreased pH and increased the production of short-chain fatty acids in colonic fermentation media.					
33934170	3	33	theme	relative	723:730	arg1	abundance					732:740	the relative abundance	719:740	the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus	719:790	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	0	34	theme	Microbiota	111:120	arg1	Composition					59:69	Composition	59:69	Composition	59:69	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	0	34	theme	Microbiota	111:120	arg1	Activity					85:92	Metabolic Activity	75:92	Metabolic Activity	75:92	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	7	35	theme	colonic	1452:1458	arg1	microbiota					1460:1469	human colonic microbiota	1446:1469	human colonic microbiota	1446:1469	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	0	36	theme	Cashew	10:15	arg1	by-Product					45:54	Cashew (Anacardium occidentale L.) by-Product	10:54	Cashew (Anacardium occidentale L.) by-Product	10:54	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	0	37	theme	Colonic	103:109	arg1	Microbiota					111:120	Human Colonic Microbiota	97:120	Human Colonic Microbiota	97:120	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	2	38	from	changes	475:481	arg1	pH					591:592	pH	591:592	pH	591:592	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	38	from	changes	475:481	arg1	abundance					495:503	relative abundance	486:503	relative abundance of distinct microbial groups found as part of human colonic microbiota	486:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	6	39	located	found	1330:1334	arg2	fructans					1288:1295	fructans	1288:1295	fructans	1288:1295	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	6	39	located	found	1330:1334	arg1	FCAB					1339:1342	FCAB	1339:1342	FCAB	1339:1342	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	3	40	theme	histolyticum	950:961	arg1	abundance					854:862	the relative abundance	841:862	the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum	841:961	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	4	41	theme	bacteria	1005:1012	arg1	counts					983:988	the counts	979:988	the counts of lactic acid bacteria	979:1012	FCAB increased the counts of lactic acid bacteria and decreased the counts of Enterobacteriaceae during colonic fermentation.					
33934170	3	42	theme	relative	845:852	arg1	abundance					854:862	the relative abundance	841:862	the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum	841:961	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	2	43	theme	CAB	400:402	arg1	capability					373:382	the capability	369:382	the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula	369:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	3	44	theme	Bifidobacterium	745:759	arg1	abundance					732:740	the relative abundance	719:740	the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus	719:790	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	2	45	theme	groups	527:532	arg1	abundance					495:503	relative abundance	486:503	relative abundance of distinct microbial groups found as part of human colonic microbiota	486:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	7	46	theme	human	1446:1450	arg1	microbiota					1460:1469	human colonic microbiota	1446:1469	human colonic microbiota	1446:1469	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	2	47	theme	pooled	676:681	arg1	inocula					695:701	a pooled human fecal inocula	674:701	a pooled human fecal inocula	674:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	48	theme	freeze-dried	387:398	arg1	CAB					400:402	freeze-dried CAB	387:402	freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula	387:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	48	theme	freeze-dried	387:398	arg1	FCAB					405:408	FCAB	405:408	FCAB	405:408	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	49	theme	gastrointestinal	436:451	arg1	digestion					453:461	a simulated gastrointestinal digestion	424:461	a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula	424:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	50	theme	microbiota	565:574	arg1	part					543:546	part	543:546	part of human colonic microbiota	543:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	4	51	theme	acid	1000:1003	arg1	bacteria					1005:1012	lactic acid bacteria	993:1012	lactic acid bacteria	993:1012	FCAB increased the counts of lactic acid bacteria and decreased the counts of Enterobacteriaceae during colonic fermentation.					
33934170	7	52	theme	metabolic	1424:1432	arg1	activity					1434:1441	metabolic activity	1424:1441	metabolic activity	1424:1441	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	1	53	theme	bioactive	327:335	arg1	compounds					337:345	bioactive compounds	327:345	bioactive compounds	327:345	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	2	54	theme	colonic	557:563	arg1	microbiota					565:574	human colonic microbiota	551:574	human colonic microbiota	551:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	7	55	theme	positive	1384:1391	arg1	alterations					1393:1403	positive alterations	1384:1403	positive alterations in composition and metabolic activity of human colonic microbiota	1384:1469	These results showed that FCAB induced positive alterations in composition and metabolic activity of human colonic microbiota in vitro, which indicate prebiotic properties.					
33934170	4	56	theme	lactic	993:998	arg1	bacteria					1005:1012	lactic acid bacteria	993:1012	lactic acid bacteria	993:1012	FCAB increased the counts of lactic acid bacteria and decreased the counts of Enterobacteriaceae during colonic fermentation.					
33934170	1	57	theme	variety	302:308	arg1	source					290:295	source	290:295	source of a variety of nutrients and bioactive compounds	290:345	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	57	theme	variety	302:308	arg1	it					261:262	it	261:262	it	261:262	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	2	58	theme	human	551:555	arg1	microbiota					565:574	human colonic microbiota	551:574	human colonic microbiota	551:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	6	59	theme	fibers	1261:1266	arg1	contents					1241:1248	contents	1241:1248	contents of dietary fibers	1241:1266	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	6	59	theme	fibers	1261:1266	arg1	presence					1276:1283	the presence	1272:1283	the presence of fructans and different phenolic compounds found in FCAB	1272:1342	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	1	60	theme	important	199:207	arg1	waste					225:229	an important agro-industrial waste	196:229	an important agro-industrial waste	196:229	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	1	61	dep	underutilized	237:249	arg1	waste					225:229	an important agro-industrial waste	196:229	an important agro-industrial waste	196:229	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	6	62	theme	dietary	1253:1259	arg1	fibers					1261:1266	dietary fibers	1253:1266	dietary fibers	1253:1266	These effects could be linked to contents of dietary fibers and the presence of fructans and different phenolic compounds found in FCAB.					
33934170	2	63	from	production	621:630	arg1	pH					591:592	pH	591:592	pH	591:592	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	2	63	from	production	621:630	arg1	abundance					495:503	relative abundance	486:503	relative abundance of distinct microbial groups found as part of human colonic microbiota	486:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	1	64	theme	agro-industrial	209:223	arg1	waste					225:229	an important agro-industrial waste	196:229	an important agro-industrial waste	196:229	Cashew apple by-product (CAB) is an important agro-industrial waste still underutilized, although it has been characterized as source of a variety of nutrients and bioactive compounds.					
33934170	2	65	theme	relative	486:493	arg1	abundance					495:503	relative abundance	486:503	relative abundance of distinct microbial groups found as part of human colonic microbiota	486:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	5	66	theme	fermentation	1188:1199	arg1	media					1201:1205	colonic fermentation media	1180:1205	colonic fermentation media	1180:1205	Furthermore, FCAB decreased pH and increased the production of short-chain fatty acids in colonic fermentation media.					
33934170	2	67	dep	in	646:647	arg1	vitro					649:653	vitro	649:653	vitro	649:653	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	3	68	theme	Lactobacillus/Enterococcus	765:790	arg1	abundance					732:740	the relative abundance	719:740	the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus	719:790	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	0	69	theme	by-Product	45:54	arg1	Impact					0:5	Impact	0:5	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro	0:129	Impact of Cashew (Anacardium occidentale L.) by-Product on Composition and Metabolic Activity of Human Colonic Microbiota In Vitro Indicates Prebiotic Properties.					
33934170	5	70	theme	acids	1171:1175	arg1	production					1139:1148	the production	1135:1148	the production of short-chain fatty acids in colonic fermentation media	1135:1205	Furthermore, FCAB decreased pH and increased the production of short-chain fatty acids in colonic fermentation media.					
33934170	2	71	theme	fecal	689:693	arg1	inocula					695:701	a pooled human fecal inocula	674:701	a pooled human fecal inocula	674:701	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
33934170	3	72	theme	colonic	799:805	arg1	fermentation					807:818	colonic fermentation	799:818	colonic fermentation	799:818	FCAB increased the relative abundance of Bifidobacterium and Lactobacillus/Enterococcus during colonic fermentation, besides to decrease the relative abundance of Bacteroides/Prevotella, Eubacterium rectale/Clostridium coccoides, and Clostridium histolyticum.					
33934170	2	73	theme	distinct	508:515	arg1	groups					527:532	distinct microbial groups	508:532	distinct microbial groups found as part of human colonic microbiota	508:574	This study evaluated the capability of freeze-dried CAB (FCAB) submitted to a simulated gastrointestinal digestion of inducing changes in relative abundance of distinct microbial groups found as part of human colonic microbiota, as well as in pH and short-chain fatty acid production during a 24-h in vitro fermentation using a pooled human fecal inocula.					
34287876	8	0	dep	APPLICATION	1872:1882	arg1	caused					1935:1940	caused	1935:1940	caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro	1935:2057	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	0	1	theme	colonic	147:153	arg1	microbiota					155:164	human colonic microbiota	141:164	human colonic microbiota	141:164	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	5	2	theme	fermentation	1267:1278	arg1	hr					1253:1254	24 and 48 hr	1243:1254	24 and 48 hr of colonic fermentation	1243:1278	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	4	3	dep	range	1115:1119	arg1	%					1131:1131	0.28-3.86%	1122:1131	range: 0.28-3.86%	1115:1131	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	2	4	theme	groups	516:521	arg1	abundance					483:491	relative abundance	474:491	relative abundance of different bacterial groups found as part of human colonic microbiota	474:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	7	5	theme	examined	1627:1634	arg1	capable					1652:1658	capable	1652:1658	capable	1652:1658	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	7	5	theme	examined	1627:1634	arg1	flours					1640:1645	The four examined SPR flours	1618:1645	The four examined SPR flours	1618:1645	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	1	6	dep	composition	333:343	arg1	the					329:331	the	329:331	the	329:331	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	7	from	varieties	263:271	arg1	flours					212:217	flours	212:217	flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel,	212:324	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	7	from	varieties	263:271	arg1	effects					201:207	the effects	197:207	the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro	197:403	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	8	theme	colonic	377:383	arg1	microbiota					385:394	human colonic microbiota	371:394	human colonic microbiota	371:394	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	5	9	contain	had	1197:1199	arg1	flours					1190:1195	The four SPRF flours	1176:1195	The four SPRF flours	1176:1195	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	5	9	contain	had	1197:1199	arg2	> 0.38					1229:1234	> 0.38	1229:1234	> 0.38	1229:1234	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	5	9	contain	had	1197:1199	arg2	indexes					1220:1226	positive prebiotic indexes	1201:1226	positive prebiotic indexes (> 0.38)	1201:1235	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	6	10	theme	colonic	1405:1411	arg1	microbiota					1413:1422	human colonic microbiota	1399:1422	human colonic microbiota	1399:1422	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	1	11	theme	sweet	239:243	arg1	varieties					263:271	four different sweet potato root (SPR) varieties	224:271	four different sweet potato root (SPR) varieties	224:271	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	2	12	theme	colonic	546:552	arg1	microbiota					554:563	human colonic microbiota	540:563	human colonic microbiota	540:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	8	13	theme	human	2025:2029	arg1	microbiota					2039:2048	human colonic microbiota	2025:2048	human colonic microbiota	2025:2048	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	8	14	theme	microbiota	2039:2048	arg1	activity					2013:2020	the metabolic activity	1999:2020	the metabolic activity of human colonic microbiota	1999:2048	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	4	15	dep	range	919:923	arg1	%					935:935	0.32-3.27%	926:935	range: 0.32-3.27%	919:935	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	6	16	theme	metabolic	1377:1385	arg1	activity					1387:1394	An increased metabolic activity	1364:1394	An increased metabolic activity of human colonic microbiota	1364:1422	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	4	17	dep	Bifidobacterium	902:916	arg1	range					919:923	range	919:923	range: 0.32-3.27%	919:935	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	2	18	theme	colonic	672:678	arg1	fermentation					680:691	an in vitro colonic fermentation	660:691	an in vitro colonic fermentation	660:691	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	4	19	dep	Lactobacillus/Enterococcus	851:876	arg1	range					879:883	range	879:883	range: 0.49-4.48%	879:895	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	4	20	theme	relative	829:836	arg1	abundance					838:846	the relative abundance	825:846	the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%)	825:936	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	6	21	theme	acid	1533:1536	arg1	production					1565:1574	increased lactic acid and short chain fatty acid production	1516:1574	increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation	1516:1615	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	2	22	theme	in	663:664	arg1	fermentation					680:691	an in vitro colonic fermentation	660:691	an in vitro colonic fermentation	660:691	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	5	23	from	microbiota	1352:1361	arg1	occurrence					1297:1306	the occurrence	1293:1306	the occurrence of selective stimulatory effects on colonic microbiota	1293:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	2	24	theme	different	496:504	arg1	groups					516:521	different bacterial groups	496:521	different bacterial groups found as part of human colonic microbiota	496:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	6	25	theme	increased	1516:1524	arg1	production					1565:1574	increased lactic acid and short chain fatty acid production	1516:1574	increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation	1516:1615	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	2	26	theme	fatty	608:612	arg1	acid					614:617	short-chain fatty acid	596:617	short-chain fatty acid	596:617	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	0	27	theme	metabolic	119:127	arg1	activity					129:136	metabolic activity	119:136	metabolic activity	119:136	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	4	28	dep	range	1002:1006	arg1	%					1018:1018	0.29-7.49%	1009:1018	range: 0.29-7.49%	1002:1018	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	4	29	theme	SPR	804:806	arg1	flours					808:813	The four SPR flours	795:813	The four SPR flours	795:813	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	3	30	theme	SPR	698:700	arg1	flours					702:707	The SPR flours	694:707	The SPR flours	694:707	The SPR flours were submitted to a simulated gastrointestinal digestion prior to use in experiments.					
34287876	4	31	dep	range	879:883	arg1	%					895:895	0.49-4.48%	886:895	range: 0.49-4.48%	879:895	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	1	32	theme	metabolic	349:357	arg1	activity					359:366	metabolic activity	349:366	metabolic activity	349:366	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	3	33	theme	simulated	729:737	arg1	digestion					756:764	a simulated gastrointestinal digestion	727:764	a simulated gastrointestinal digestion prior to use in experiments	727:792	The SPR flours were submitted to a simulated gastrointestinal digestion prior to use in experiments.					
34287876	2	34	theme	SPR	430:432	arg1	flours					434:439	these SPR flours	424:439	these SPR flours	424:439	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	3	35	theme	prior	766:770	arg1	digestion					756:764	a simulated gastrointestinal digestion	727:764	a simulated gastrointestinal digestion prior to use in experiments	727:792	The SPR flours were submitted to a simulated gastrointestinal digestion prior to use in experiments.					
34287876	0	36	theme	potato	53:58	arg1	Ipomoea					61:67	Ipomoea	61:67	Ipomoea	61:67	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	36	theme	potato	53:58	arg1	varieties					86:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	8	37	theme	PRACTICAL	1862:1870	arg1	APPLICATION					1872:1882	PRACTICAL APPLICATION	1862:1882	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.	1862:2058	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	0	38	dep	composition	103:113	arg1	the					99:101	the	99:101	the	99:101	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	5	39	theme	effects	1333:1339	arg1	occurrence					1297:1306	the occurrence	1293:1306	the occurrence of selective stimulatory effects on colonic microbiota	1293:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	1	40	from	effects	201:207	arg1	varieties					263:271	four different sweet potato root (SPR) varieties	224:271	four different sweet potato root (SPR) varieties	224:271	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	40	from	effects	201:207	arg1	composition					333:343	composition	333:343	composition	333:343	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	40	from	effects	201:207	arg1	activity					359:366	metabolic activity	349:366	metabolic activity	349:366	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	41	theme	white	289:293	arg1	peel					295:298	white peel	289:298	white peel	289:298	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	42	from	Effects	0:6	arg1	composition					103:113	composition	103:113	composition	103:113	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	42	from	Effects	0:6	arg1	activity					129:136	metabolic activity	119:136	metabolic activity	119:136	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	42	from	Effects	0:6	arg1	Ipomoea					61:67	Ipomoea	61:67	Ipomoea	61:67	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	42	from	Effects	0:6	arg1	varieties					86:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	5	43	theme	colonic	1344:1350	arg1	microbiota					1352:1361	colonic microbiota	1344:1361	colonic microbiota	1344:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	8	44	theme	metabolic	2003:2011	arg1	activity					2013:2020	the metabolic activity	1999:2020	the metabolic activity of human colonic microbiota	1999:2048	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	6	45	theme	fatty	1554:1558	arg1	acid					1560:1563	short chain fatty acid	1542:1563	short chain fatty acid	1542:1563	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	2	46	theme	microbiota	554:563	arg1	part					532:535	part	532:535	part of human colonic microbiota	532:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	0	47	theme	root	81:84	arg1	Ipomoea					61:67	Ipomoea	61:67	Ipomoea	61:67	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	47	theme	root	81:84	arg1	varieties					86:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	4	48	dep	Bacteroides/Prevotella	978:999	arg1	range					1002:1006	range	1002:1006	range: 0.29-7.49%	1002:1018	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	8	49	theme	sweet	1903:1907	arg1	SPRF					1929:1932	SPRF	1929:1932	SPRF	1929:1932	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	8	49	theme	sweet	1903:1907	arg1	flours					1921:1926	The four examined sweet potato root flours	1885:1926	The four examined sweet potato root flours (SPRF)	1885:1933	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	7	50	dep	in	1777:1778	arg1	vitro					1780:1784	vitro	1780:1784	vitro	1780:1784	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	6	51	theme	short	1542:1546	arg1	acid					1560:1563	short chain fatty acid	1542:1563	short chain fatty acid	1542:1563	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	5	52	theme	selective	1311:1319	arg1	effects					1333:1339	selective stimulatory effects	1311:1339	selective stimulatory effects on colonic microbiota	1311:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	2	53	theme	lactic	580:585	arg1	acid					587:590	lactic acid	580:590	lactic acid	580:590	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	5	54	theme	positive	1201:1208	arg1	> 0.38					1229:1234	> 0.38	1229:1234	> 0.38	1229:1234	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	5	54	theme	positive	1201:1208	arg1	indexes					1220:1226	positive prebiotic indexes	1201:1226	positive prebiotic indexes (> 0.38)	1201:1235	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	1	55	theme	potato	245:250	arg1	varieties					263:271	four different sweet potato root (SPR) varieties	224:271	four different sweet potato root (SPR) varieties	224:271	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	7	56	theme	colonic	1751:1757	arg1	microbiota					1759:1768	human colonic microbiota	1745:1768	human colonic microbiota	1745:1768	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	0	57	dep	Ipomoea	61:67	arg1	L.					77:78	Ipomoea batatas L.	61:78	Ipomoea batatas L.	61:78	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	6	58	dep	pH	1490:1491	arg1	range					1494:1498	range	1494:1498	range: 3.20-3.83	1494:1509	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	8	59	theme	root	1916:1919	arg1	SPRF					1929:1932	SPRF	1929:1932	SPRF	1929:1932	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	8	59	theme	root	1916:1919	arg1	flours					1921:1926	The four examined sweet potato root flours	1885:1926	The four examined sweet potato root flours (SPRF)	1885:1933	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	9	60	theme	prebiotic	2130:2138	arg1	ingredients					2140:2150	prebiotic ingredients	2130:2150	prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements	2130:2238	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	0	61	theme	digested	11:18	arg1	flours					20:25	digested flours	11:25	digested flours from four different sweet potato (Ipomoea batatas L.) root varieties	11:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	6	62	dep	range	1494:1498	arg1	3.20-3.83					1501:1509	3.20-3.83	1501:1509	3.20-3.83	1501:1509	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	6	63	theme	human	1399:1403	arg1	microbiota					1413:1422	human colonic microbiota	1399:1422	human colonic microbiota	1399:1422	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	7	64	theme	metabolic	1723:1731	arg1	activity					1733:1740	the metabolic activity	1719:1740	the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties	1719:1859	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	7	65	theme	positive	1671:1678	arg1	alterations					1680:1690	positive alterations	1671:1690	positive alterations in composition	1671:1705	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	7	66	from	alterations	1680:1690	arg1	composition					1695:1705	composition	1695:1705	composition	1695:1705	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	6	67	theme	colonic	1596:1602	arg1	fermentation					1604:1615	colonic fermentation	1596:1615	colonic fermentation	1596:1615	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	0	68	theme	different	37:45	arg1	Ipomoea					61:67	Ipomoea	61:67	Ipomoea	61:67	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	68	theme	different	37:45	arg1	varieties					86:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	2	69	dep	capability	410:419	arg1	cause					453:457	cause	453:457	to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production	450:628	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	8	70	theme	beneficial	1942:1951	arg1	alterations					1953:1963	beneficial alterations	1942:1963	beneficial alterations in composition	1942:1978	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	4	71	theme	Bacteroides/Prevotella	978:999	arg1	abundance					965:973	the relative abundance	952:973	the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%)	952:1132	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	9	72	theme	food	2155:2158	arg1	industry					2160:2167	food industry	2155:2167	food industry	2155:2167	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	9	73	theme	value-added	2188:2198	arg1	foods					2211:2215	value-added functional foods	2188:2215	value-added functional foods	2188:2215	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	8	74	theme	examined	1894:1901	arg1	SPRF					1929:1932	SPRF	1929:1932	SPRF	1929:1932	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	8	74	theme	examined	1894:1901	arg1	flours					1921:1926	The four examined sweet potato root flours	1885:1926	The four examined sweet potato root flours (SPRF)	1885:1933	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	7	75	theme	SPR	1636:1638	arg1	capable					1652:1658	capable	1652:1658	capable	1652:1658	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	7	75	theme	SPR	1636:1638	arg1	flours					1640:1645	The four examined SPR flours	1618:1645	The four examined SPR flours	1618:1645	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	2	76	from	alterations	459:469	arg1	abundance					483:491	relative abundance	474:491	relative abundance of different bacterial groups found as part of human colonic microbiota	474:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	2	76	from	alterations	459:469	arg1	production					619:628	lactic acid and short-chain fatty acid production	580:628	lactic acid and short-chain fatty acid production	580:628	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	0	77	theme	microbiota	155:164	arg1	composition					103:113	composition	103:113	composition	103:113	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	77	theme	microbiota	155:164	arg1	activity					129:136	metabolic activity	119:136	metabolic activity	119:136	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	9	78	theme	foods	2211:2215	arg1	formulation					2173:2183	formulation	2173:2183	formulation of value-added functional foods or dietary supplements	2173:2238	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	1	79	theme	human	371:375	arg1	microbiota					385:394	human colonic microbiota	371:394	human colonic microbiota	371:394	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	9	80	theme	dietary	2220:2226	arg1	supplements					2228:2238	dietary supplements	2220:2238	dietary supplements	2220:2238	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	1	81	theme	different	229:237	arg1	varieties					263:271	four different sweet potato root (SPR) varieties	224:271	four different sweet potato root (SPR) varieties	224:271	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	2	82	theme	bacterial	506:514	arg1	groups					516:521	different bacterial groups	496:521	different bacterial groups found as part of human colonic microbiota	496:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	8	83	from	alterations	1953:1963	arg1	composition					1968:1978	composition	1968:1978	composition	1968:1978	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	3	84	from	use	775:777	arg1	experiments					782:792	experiments	782:792	experiments	782:792	The SPR flours were submitted to a simulated gastrointestinal digestion prior to use in experiments.					
34287876	4	85	theme	fermentation	1162:1173	arg1	hr					1148:1149	the 48 hr	1141:1149	the 48 hr of colonic fermentation	1141:1173	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	1	86	theme	microbiota	385:394	arg1	composition					333:343	composition	333:343	composition	333:343	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	86	theme	microbiota	385:394	arg1	activity					359:366	metabolic activity	349:366	metabolic activity	349:366	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	4	87	dep	range	1048:1052	arg1	%					1064:1064	0.15-2.08%	1055:1064	range: 0.15-2.08%	1048:1064	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	9	88	theme	supplements	2228:2238	arg1	formulation					2173:2183	formulation	2173:2183	formulation of value-added functional foods or dietary supplements	2173:2238	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	2	89	theme	human	540:544	arg1	microbiota					554:563	human colonic microbiota	540:563	human colonic microbiota	540:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	4	90	dep	histolyticum	1034:1045	arg1	range					1048:1052	range	1048:1052	range: 0.15-2.08%	1048:1064	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	7	91	theme	prebiotic	1840:1848	arg1	properties					1850:1859	their prebiotic properties	1834:1859	their prebiotic properties	1834:1859	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	8	92	theme	colonic	2031:2037	arg1	microbiota					2039:2048	human colonic microbiota	2025:2048	human colonic microbiota	2025:2048	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	2	93	theme	fermentation	680:691	arg1	hr					654:655	48 hr	651:655	48 hr of an in vitro colonic fermentation	651:691	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	5	94	theme	colonic	1259:1265	arg1	fermentation					1267:1278	colonic fermentation	1259:1278	colonic fermentation	1259:1278	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	6	95	theme	increased	1367:1375	arg1	activity					1387:1394	An increased metabolic activity	1364:1394	An increased metabolic activity of human colonic microbiota	1364:1422	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	5	96	from	occurrence	1297:1306	arg1	microbiota					1352:1361	colonic microbiota	1344:1361	colonic microbiota	1344:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	6	97	theme	lactic	1526:1531	arg1	acid					1533:1536	lactic acid	1526:1536	lactic acid	1526:1536	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	2	98	theme	acid	614:617	arg1	production					619:628	lactic acid and short-chain fatty acid production	580:628	lactic acid and short-chain fatty acid production	580:628	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	5	99	from	effects	1333:1339	arg1	microbiota					1352:1361	colonic microbiota	1344:1361	colonic microbiota	1344:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	0	100	from	varieties	86:94	arg1	Effects					0:6	Effects	0:6	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.	0:174	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	100	from	varieties	86:94	arg1	flours					20:25	digested flours	11:25	digested flours from four different sweet potato (Ipomoea batatas L.) root varieties	11:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	2	101	theme	short-chain	596:606	arg1	acid					614:617	short-chain fatty acid	596:617	short-chain fatty acid	596:617	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	7	102	attach	linked	1824:1829	arg2	fermentation					1794:1805	in vitro colonic fermentation	1777:1805	in vitro colonic fermentation	1777:1805	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	7	102	attach	linked	1824:1829	arg1	properties					1850:1859	their prebiotic properties	1834:1859	their prebiotic properties	1834:1859	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	0	103	theme	human	141:145	arg1	microbiota					155:164	human colonic microbiota	141:164	human colonic microbiota	141:164	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	6	104	theme	tested	1438:1443	arg1	flours					1449:1454	tested SPR flours	1438:1454	tested SPR flours	1438:1454	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	6	105	theme	SPR	1445:1447	arg1	flours					1449:1454	tested SPR flours	1438:1454	tested SPR flours	1438:1454	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	2	106	theme	relative	474:481	arg1	abundance					483:491	relative abundance	474:491	relative abundance of different bacterial groups found as part of human colonic microbiota	474:563	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	3	107	theme	gastrointestinal	739:754	arg1	digestion					756:764	a simulated gastrointestinal digestion	727:764	a simulated gastrointestinal digestion prior to use in experiments	727:792	The SPR flours were submitted to a simulated gastrointestinal digestion prior to use in experiments.					
34287876	7	108	theme	colonic	1786:1792	arg1	fermentation					1794:1805	in vitro colonic fermentation	1777:1805	in vitro colonic fermentation	1777:1805	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	6	109	theme	microbiota	1413:1422	arg1	activity					1387:1394	An increased metabolic activity	1364:1394	An increased metabolic activity of human colonic microbiota	1364:1422	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	9	110	theme	examined	2091:2098	arg1	candidates					2108:2117	candidates	2108:2117	candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements	2108:2238	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	9	110	theme	examined	2091:2098	arg1	SPRF					2100:2103	the examined SPRF	2087:2103	the examined SPRF	2087:2103	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	7	111	theme	in	1777:1778	arg1	fermentation					1794:1805	in vitro colonic fermentation	1777:1805	in vitro colonic fermentation	1777:1805	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	6	112	theme	acid	1560:1563	arg1	production					1565:1574	increased lactic acid and short chain fatty acid production	1516:1574	increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation	1516:1615	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	5	113	theme	SPRF	1185:1188	arg1	flours					1190:1195	The four SPRF flours	1176:1195	The four SPRF flours	1176:1195	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	7	114	theme	microbiota	1759:1768	arg1	activity					1733:1740	the metabolic activity	1719:1740	the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties	1719:1859	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	6	115	theme	decreased	1480:1488	arg1	pH					1490:1491	decreased pH	1480:1491	decreased pH (range: 3.20-3.83)	1480:1510	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	8	116	theme	potato	1909:1914	arg1	SPRF					1929:1932	SPRF	1929:1932	SPRF	1929:1932	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	8	116	theme	potato	1909:1914	arg1	flours					1921:1926	The four examined sweet potato root flours	1885:1926	The four examined sweet potato root flours (SPRF)	1885:1933	PRACTICAL APPLICATION: The four examined sweet potato root flours (SPRF) caused beneficial alterations in composition besides of driving the metabolic activity of human colonic microbiota in vitro.					
34287876	4	117	theme	Lactobacillus/Enterococcus	851:876	arg1	abundance					838:846	the relative abundance	825:846	the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%)	825:936	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	6	118	theme	chain	1548:1552	arg1	acid					1560:1563	short chain fatty acid	1542:1563	short chain fatty acid	1542:1563	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	2	119	theme	acid	587:590	arg1	production					619:628	lactic acid and short-chain fatty acid production	580:628	lactic acid and short-chain fatty acid production	580:628	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	1	120	theme	purple	313:318	arg1	peel					320:323	purple peel	313:323	purple peel	313:323	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	7	121	theme	human	1745:1749	arg1	microbiota					1759:1768	human colonic microbiota	1745:1768	human colonic microbiota	1745:1768	The four examined SPR flours were capable of causing positive alterations in composition and driving the metabolic activity of human colonic microbiota during in vitro colonic fermentation, which should be linked to their prebiotic properties.					
34287876	5	122	theme	stimulatory	1321:1331	arg1	effects					1333:1339	selective stimulatory effects	1311:1339	selective stimulatory effects on colonic microbiota	1311:1361	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	0	123	theme	flours	20:25	arg1	Effects					0:6	Effects	0:6	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.	0:174	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	124	theme	root	252:255	arg1	varieties					263:271	four different sweet potato root (SPR) varieties	224:271	four different sweet potato root (SPR) varieties	224:271	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	1	125	theme	SPR	258:260	arg1	varieties					263:271	four different sweet potato root (SPR) varieties	224:271	four different sweet potato root (SPR) varieties	224:271	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	6	126	theme	fermentation	1604:1615	arg1	pH					1490:1491	decreased pH	1480:1491	decreased pH (range: 3.20-3.83)	1480:1510	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	6	126	theme	fermentation	1604:1615	arg1	production					1565:1574	increased lactic acid and short chain fatty acid production	1516:1574	increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation	1516:1615	An increased metabolic activity of human colonic microbiota was caused by tested SPR flours, which was evidenced by decreased pH (range: 3.20-3.83) and increased lactic acid and short chain fatty acid production during the 48 hr of colonic fermentation.					
34287876	4	127	theme	colonic	1154:1160	arg1	fermentation					1162:1173	colonic fermentation	1154:1173	colonic fermentation	1154:1173	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	2	128	theme	flours	434:439	arg1	capability					410:419	The capability	406:419	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production	406:628	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	2	128	theme	flours	434:439	arg1	20 g/L					442:447	20 g/L	442:447	20 g/L	442:447	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	0	129	theme	sweet	47:51	arg1	Ipomoea					61:67	Ipomoea	61:67	Ipomoea	61:67	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	0	129	theme	sweet	47:51	arg1	varieties					86:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	four different sweet potato (Ipomoea batatas L.) root varieties	32:94	Effects of digested flours from four different sweet potato (Ipomoea batatas L.) root varieties on the composition and metabolic activity of human colonic microbiota in vitro.					
34287876	2	130	dep	in	663:664	arg1	vitro					666:670	vitro	666:670	vitro	666:670	The capability of these SPR flours (20 g/L) to cause alterations in relative abundance of different bacterial groups found as part of human colonic microbiota, as well as in lactic acid and short-chain fatty acid production was evaluated during 48 hr of an in vitro colonic fermentation.					
34287876	4	131	theme	Bifidobacterium	902:916	arg1	abundance					838:846	the relative abundance	825:846	the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%)	825:936	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	4	132	dep	coccoides	1104:1112	arg1	range					1115:1119	range	1115:1119	range: 0.28-3.86%	1115:1131	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	4	133	theme	relative	956:963	arg1	abundance					965:973	the relative abundance	952:973	the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%)	952:1132	The four SPR flours increased the relative abundance of Lactobacillus/Enterococcus (range: 0.49-4.48%) and Bifidobacterium (range: 0.32-3.27%) and decreased the relative abundance of Bacteroides/Prevotella (range: 0.29-7.49%), Clostridium histolyticum (range: 0.15-2.08%), and Eubacterium rectale/Clostridium coccoides (range: 0.28-3.86%) during the 48 hr of colonic fermentation.					
34287876	5	134	theme	prebiotic	1210:1218	arg1	> 0.38					1229:1234	> 0.38	1229:1234	> 0.38	1229:1234	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	5	134	theme	prebiotic	1210:1218	arg1	indexes					1220:1226	positive prebiotic indexes	1201:1226	positive prebiotic indexes (> 0.38)	1201:1235	The four SPRF flours had positive prebiotic indexes (> 0.38) after 24 and 48 hr of colonic fermentation, reinforcing the occurrence of selective stimulatory effects on colonic microbiota.					
34287876	9	135	theme	functional	2200:2209	arg1	foods					2211:2215	value-added functional foods	2188:2215	value-added functional foods	2188:2215	These results characterize the examined SPRF as candidates for use as prebiotic ingredients by food industry for formulation of value-added functional foods or dietary supplements.					
34287876	1	136	theme	flours	212:217	arg1	effects					201:207	the effects	197:207	the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro	197:403	This study evaluated the effects of flours from four different sweet potato root (SPR) varieties, being two with white peel and two with purple peel, on the composition and metabolic activity of human colonic microbiota in vitro.					
33105554	9	0	theme	acid	1143:1146	arg1	profile					1148:1154	a healthier cecal short-chain fatty acid profile	1107:1154	a healthier cecal short-chain fatty acid profile	1107:1154	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	2	1	from	NAFLD	416:420	arg1	mice					425:428	mice	425:428	mice	425:428	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	1	2	theme	liver	171:175	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	2	theme	liver	171:175	arg1	disease					177:183	non-alcoholic fatty liver disease	151:183	non-alcoholic fatty liver disease (NAFLD)	151:191	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	3	from	importance	110:119	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	3	from	importance	110:119	arg1	disease					177:183	non-alcoholic fatty liver disease	151:183	non-alcoholic fatty liver disease (NAFLD)	151:191	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	4	4	theme	XOS	552:554	arg1	effect					542:547	the effect	538:547	the effect of XOS on F. prausnitzii growth	538:579	First, the effect of XOS on F. prausnitzii growth was assessed in vitro.					
33105554	5	5	theme	low	681:683	arg1	LFD					686:688	LFD	686:688	LFD	686:688	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	5	theme	low	681:683	arg1	diet					695:698	high (HFD, 60% of energy from fat) or low (LFD) fat diet	643:698	diet	695:698	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	9	6	theme	metabolites	1043:1053	arg1	analysis					1025:1032	Nuclear magnetic resonance (1H-NMR) analysis	989:1032	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites	989:1053	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	7	theme	cecal	1119:1123	arg1	profile					1148:1154	a healthier cecal short-chain fatty acid profile	1107:1154	a healthier cecal short-chain fatty acid profile	1107:1154	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	8	attach	linked	1239:1244	arg2	metabolites					1216:1226	metabolites	1216:1226	metabolites previously linked to NAFLD	1216:1253	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	8	attach	linked	1239:1244	arg1	NAFLD					1249:1253	NAFLD	1249:1253	NAFLD	1249:1253	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	8	attach	linked	1239:1244	arg2	isovalerate					1190:1200	cecal isovalerate	1184:1200	cecal isovalerate	1184:1200	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	6	9	theme	prausnitzii	761:771	arg1	growth					773:778	F. prausnitzii growth	758:778	F. prausnitzii growth	758:778	XOS increased F. prausnitzii growth, having only a minor impact on the GM composition.					
33105554	2	10	from	abundance	303:311	arg1	humans					348:353	humans	348:353	humans	348:353	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	4	11	theme	F.	559:560	arg1	growth					574:579	F. prausnitzii growth	559:579	F. prausnitzii growth	559:579	First, the effect of XOS on F. prausnitzii growth was assessed in vitro.					
33105554	5	12	dep	high	643:646	arg1	energy					661:666	energy	661:666	energy from fat	661:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	12	dep	high	643:646	arg1	HFD					649:651	HFD	649:651	HFD	649:651	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	12	dep	high	643:646	arg1	%					656:656	60%	654:656	60% of energy from fat	654:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	6	13	theme	F.	758:759	arg1	growth					773:778	F. prausnitzii growth	758:778	F. prausnitzii growth	758:778	XOS increased F. prausnitzii growth, having only a minor impact on the GM composition.					
33105554	5	14	theme	energy	661:666	arg1	energy					661:666	energy	661:666	energy from fat	661:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	14	theme	energy	661:666	arg1	HFD					649:651	HFD	649:651	HFD	649:651	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	14	theme	energy	661:666	arg1	%					656:656	60%	654:656	60% of energy from fat	654:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	15	from	weeks	707:711	arg1	rats					723:726	Wistar rats	716:726	Wistar rats (n = 10/group)	716:741	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	15	from	weeks	707:711	arg1	n					729:729	n = 10/group	729:740	n = 10/group	729:740	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	10	16	theme	hepatic	1340:1346	arg1	triglycerides					1348:1360	hepatic triglycerides	1340:1360	hepatic triglycerides	1340:1360	Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides.					
33105554	2	17	theme	fat	271:273	arg1	content					275:281	high hepatic fat content	258:281	high hepatic fat content	258:281	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	2	18	theme	F.	391:392	arg1	prausnitzii					394:404	F. prausnitzii	391:404	F. prausnitzii	391:404	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	5	19	theme	fat	691:693	arg1	LFD					686:688	LFD	686:688	LFD	686:688	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	19	theme	fat	691:693	arg1	diet					695:698	high (HFD, 60% of energy from fat) or low (LFD) fat diet	643:698	diet	695:698	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	0	20	from	Steatosis	73:81	arg1	Rats					86:89	Rats	86:89	Rats	86:89	Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats.					
33105554	8	21	theme	underlying	898:907	arg1	mechanisms					909:918	The underlying mechanisms	894:918	The underlying mechanisms involved	894:927	The underlying mechanisms involved enhanced hepatic β-oxidation and mitochondrial respiration.					
33105554	10	22	theme	fatty	1277:1281	arg1	acids					1283:1287	Cecal branched-chain fatty acids	1256:1287	Cecal branched-chain fatty acids	1256:1287	Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides.					
33105554	2	23	theme	hepatic	263:269	arg1	content					275:281	high hepatic fat content	258:281	high hepatic fat content	258:281	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	5	24	theme	=	731:731	arg1	rats					723:726	Wistar rats	716:726	Wistar rats (n = 10/group)	716:741	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	24	theme	=	731:731	arg1	n					729:729	n = 10/group	729:740	n = 10/group	729:740	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	4	25	from	effect	542:547	arg1	growth					574:579	F. prausnitzii growth	559:579	F. prausnitzii growth	559:579	First, the effect of XOS on F. prausnitzii growth was assessed in vitro.					
33105554	10	26	theme	branched-chain	1262:1275	arg1	acids					1283:1287	Cecal branched-chain fatty acids	1256:1287	Cecal branched-chain fatty acids	1256:1287	Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides.					
33105554	0	27	theme	Prebiotic	0:8	arg1	Xylo-Oligosaccharides					10:30	Prebiotic Xylo-Oligosaccharides	0:30	Prebiotic Xylo-Oligosaccharides	0:30	Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats.					
33105554	10	28	theme	Cecal	1256:1260	arg1	acids					1283:1287	Cecal branched-chain fatty acids	1256:1287	Cecal branched-chain fatty acids	1256:1287	Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides.					
33105554	0	29	theme	High-Fat-Diet-Induced	43:63	arg1	Steatosis					73:81	High-Fat-Diet-Induced Hepatic Steatosis	43:81	High-Fat-Diet-Induced Hepatic Steatosis in Rats	43:89	Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats.					
33105554	6	30	theme	GM	815:816	arg1	composition					818:828	the GM composition	811:828	the GM composition	811:828	XOS increased F. prausnitzii growth, having only a minor impact on the GM composition.					
33105554	11	31	theme	oxidative	1440:1448	arg1	metabolism					1450:1459	hepatic oxidative metabolism	1432:1459	hepatic oxidative metabolism	1432:1459	In conclusion, XOS supplementation can ameliorate NAFLD by improving hepatic oxidative metabolism and affecting GM.					
33105554	2	32	theme	low	299:301	arg1	abundance					303:311	low abundance	299:311	low abundance of Faecalibacterium prausnitzii in humans	299:353	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	9	33	theme	cecal	1184:1188	arg1	isovalerate					1190:1200	cecal isovalerate	1184:1200	cecal isovalerate	1184:1200	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	33	theme	cecal	1184:1188	arg1	metabolites					1216:1226	metabolites	1216:1226	metabolites previously linked to NAFLD	1216:1253	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	8	34	theme	hepatic	938:944	arg1	β-oxidation					946:956	hepatic β-oxidation	938:956	hepatic β-oxidation	938:956	The underlying mechanisms involved enhanced hepatic β-oxidation and mitochondrial respiration.					
33105554	1	35	theme	microbiota	132:141	arg1	importance					110:119	the importance	106:119	the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD)	106:191	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	9	36	theme	Nuclear	989:995	arg1	1H-NMR					1017:1022	1H-NMR	1017:1022	1H-NMR	1017:1022	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	36	theme	Nuclear	989:995	arg1	resonance					1006:1014	Nuclear magnetic resonance	989:1014	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites	989:1053	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	5	37	theme	Wistar	716:721	arg1	rats					723:726	Wistar rats	716:726	Wistar rats (n = 10/group)	716:741	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	37	theme	Wistar	716:721	arg1	n					729:729	n = 10/group	729:740	n = 10/group	729:740	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	8	38	theme	mitochondrial	962:974	arg1	respiration					976:986	mitochondrial respiration	962:986	mitochondrial respiration	962:986	The underlying mechanisms involved enhanced hepatic β-oxidation and mitochondrial respiration.					
33105554	9	39	theme	LFD	1093:1095	arg1	group					1097:1101	the LFD group	1089:1101	the LFD group	1089:1101	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	2	40	dep	prevented	406:414	arg1	associated					283:292	associated	283:292	associated with low abundance of Faecalibacterium prausnitzii in humans	283:353	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	9	41	theme	magnetic	997:1004	arg1	1H-NMR					1017:1022	1H-NMR	1017:1022	1H-NMR	1017:1022	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	41	theme	magnetic	997:1004	arg1	resonance					1006:1014	Nuclear magnetic resonance	989:1014	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites	989:1053	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	0	42	theme	Hepatic	65:71	arg1	Steatosis					73:81	High-Fat-Diet-Induced Hepatic Steatosis	43:81	High-Fat-Diet-Induced Hepatic Steatosis in Rats	43:89	Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats.					
33105554	11	43	theme	hepatic	1432:1438	arg1	metabolism					1450:1459	hepatic oxidative metabolism	1432:1459	hepatic oxidative metabolism	1432:1459	In conclusion, XOS supplementation can ameliorate NAFLD by improving hepatic oxidative metabolism and affecting GM.					
33105554	5	44	from	%	656:656	arg1	fat					673:675	fat	673:675	fat	673:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	9	45	theme	resonance	1006:1014	arg1	analysis					1025:1032	Nuclear magnetic resonance (1H-NMR) analysis	989:1032	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites	989:1053	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	2	46	theme	prausnitzii	394:404	arg1	administration					373:386	the administration	369:386	the administration of F. prausnitzii	369:404	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	6	47	theme	minor	795:799	arg1	impact					801:806	only a minor impact	788:806	only a minor impact on the GM composition	788:828	XOS increased F. prausnitzii growth, having only a minor impact on the GM composition.					
33105554	7	48	theme	hepatic	875:881	arg1	steatosis					883:891	hepatic steatosis	875:891	hepatic steatosis	875:891	When supplemented with HFD, XOS ameliorated hepatic steatosis.					
33105554	4	49	theme	prausnitzii	562:572	arg1	growth					574:579	F. prausnitzii growth	559:579	F. prausnitzii growth	559:579	First, the effect of XOS on F. prausnitzii growth was assessed in vitro.					
33105554	3	50	theme	prebiotic	477:485	arg1	XOS					510:512	XOS	510:512	XOS	510:512	Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD.					
33105554	3	50	theme	prebiotic	477:485	arg1	xylo-oligosaccharides					487:507	prebiotic xylo-oligosaccharides	477:507	prebiotic xylo-oligosaccharides (XOS)	477:513	Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD.					
33105554	9	51	contain	had	1103:1105	arg1	group					1097:1101	the LFD group	1089:1101	the LFD group	1089:1101	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	9	51	contain	had	1103:1105	arg2	profile					1148:1154	a healthier cecal short-chain fatty acid profile	1107:1154	a healthier cecal short-chain fatty acid profile	1107:1154	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	6	52	from	impact	801:806	arg1	composition					818:828	the GM composition	811:828	the GM composition	811:828	XOS increased F. prausnitzii growth, having only a minor impact on the GM composition.					
33105554	9	53	theme	healthier	1109:1117	arg1	profile					1148:1154	a healthier cecal short-chain fatty acid profile	1107:1154	a healthier cecal short-chain fatty acid profile	1107:1154	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	2	54	theme	high	258:261	arg1	content					275:281	high hepatic fat content	258:281	high hepatic fat content	258:281	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	2	55	theme	prausnitzii	333:343	arg1	abundance					303:311	low abundance	299:311	low abundance of Faecalibacterium prausnitzii in humans	299:353	We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice.					
33105554	1	56	theme	gut	128:130	arg1	GM					144:145	GM	144:145	GM	144:145	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	56	theme	gut	128:130	arg1	microbiota					132:141	the gut microbiota	124:141	the gut microbiota (GM)	124:146	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	57	theme	therapeutic	217:227	arg1	microbes					229:236	therapeutic microbes	217:236	therapeutic microbes	217:236	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	5	58	from	fat	673:675	arg1	energy					661:666	energy	661:666	energy from fat	661:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	58	from	fat	673:675	arg1	HFD					649:651	HFD	649:651	HFD	649:651	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	5	58	from	fat	673:675	arg1	%					656:656	60%	654:656	60% of energy from fat	654:675	Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group).					
33105554	3	59	theme	F.	459:460	arg1	prausnitzii					462:472	F. prausnitzii	459:472	F. prausnitzii by prebiotic xylo-oligosaccharides (XOS)	459:513	Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD.					
33105554	9	60	theme	short-chain	1125:1135	arg1	profile					1148:1154	a healthier cecal short-chain fatty acid profile	1107:1154	a healthier cecal short-chain fatty acid profile	1107:1154	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	11	61	theme	XOS	1378:1380	arg1	supplementation					1382:1396	XOS supplementation	1378:1396	XOS supplementation	1378:1396	In conclusion, XOS supplementation can ameliorate NAFLD by improving hepatic oxidative metabolism and affecting GM.					
33105554	1	62	theme	non-alcoholic	151:163	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	62	theme	non-alcoholic	151:163	arg1	disease					177:183	non-alcoholic fatty liver disease	151:183	non-alcoholic fatty liver disease (NAFLD)	151:191	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	9	63	theme	fatty	1137:1141	arg1	profile					1148:1154	a healthier cecal short-chain fatty acid profile	1107:1154	a healthier cecal short-chain fatty acid profile	1107:1154	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	1	64	dep	the	204:206	arg1	hope					208:211	hope	208:211	hope	208:211	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	9	65	theme	cecal	1037:1041	arg1	metabolites					1043:1053	cecal metabolites	1037:1053	cecal metabolites	1037:1053	Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD.					
33105554	1	66	theme	fatty	165:169	arg1	NAFLD					186:190	NAFLD	186:190	NAFLD	186:190	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
33105554	1	66	theme	fatty	165:169	arg1	disease					177:183	non-alcoholic fatty liver disease	151:183	non-alcoholic fatty liver disease (NAFLD)	151:191	Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes.					
32122492	6	0	theme	AgNP-BNCP	1063:1071	arg1	composites					1073:1082	AgNP-BNCP composites	1063:1082	AgNP-BNCP composites	1063:1082	These results demonstrate potential of AgNP-BNCP composites for user-friendly in-situ SERS analysis.					
32122492	1	1	theme	flexible	172:179	arg1	substrate					222:230	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate	156:230	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	156:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	1	2	theme	biodegradable	274:286	arg1	BNC					313:315	BNC	313:315	BNC	313:315	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	1	2	theme	biodegradable	274:286	arg1	nanocellulose					298:310	biodegradable bacterial nanocellulose	274:310	biodegradable bacterial nanocellulose (BNC)	274:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	0	3	theme	pesticides	98:107	arg1	detection					85:93	paste-and-read SERS detection	65:93	paste-and-read SERS detection of pesticides on fruit surfaces	65:125	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	1	4	theme	surface-enhanced	181:196	arg1	SERS					216:219	SERS	216:219	SERS	216:219	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	1	4	theme	surface-enhanced	181:196	arg1	scattering					204:213	surface-enhanced Raman scattering	181:213	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	156:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	1	5	theme	bacterial	288:296	arg1	BNC					313:315	BNC	313:315	BNC	313:315	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	1	5	theme	bacterial	288:296	arg1	nanocellulose					298:310	biodegradable bacterial nanocellulose	274:310	biodegradable bacterial nanocellulose (BNC)	274:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	2	6	theme	Plasmonic	319:327	arg1	AgNP-BNCP					381:389	AgNP-BNCP	381:389	AgNP-BNCP	381:389	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	2	6	theme	Plasmonic	319:327	arg1	paper					374:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper	319:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	4	7	theme	methomyl	804:811	arg1	detection					769:777	the detection	765:777	the detection of 4-aminothiophenol and methomyl pesticide	765:821	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	0	8	theme	fruit	112:116	arg1	surfaces					118:125	fruit surfaces	112:125	fruit surfaces	112:125	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	5	9	theme	SERS	1009:1012	arg1	approach					1014:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	3	10	theme	3D	590:591	arg1	hotspots					598:605	3D SERS hotspots	590:605	3D SERS hotspots within a few-micron depth on the substrate	590:648	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	4	11	dep	4-aminothiophenol	782:798	arg1	pesticide					813:821	pesticide	813:821	pesticide	813:821	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	6	12	theme	SERS	1110:1113	arg1	analysis					1115:1122	user-friendly in-situ SERS analysis	1088:1122	user-friendly in-situ SERS analysis	1088:1122	These results demonstrate potential of AgNP-BNCP composites for user-friendly in-situ SERS analysis.					
32122492	1	13	theme	Raman	198:202	arg1	SERS					216:219	SERS	216:219	SERS	216:219	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	1	13	theme	Raman	198:202	arg1	scattering					204:213	surface-enhanced Raman scattering	181:213	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	156:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	5	14	theme	BNCP	859:862	arg1	transparency					843:854	the optical transparency	831:854	the optical transparency of BNCP	831:862	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	5	15	theme	paste-and-read	993:1006	arg1	approach					1014:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	1	16	theme	scattering	204:213	arg1	substrate					222:230	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate	156:230	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	156:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	0	17	theme	nanoparticle/bacterial	7:28	arg1	composites					50:59	Silver nanoparticle/bacterial nanocellulose paper composites	0:59	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.	0:126	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	2	18	theme	nanocellulose	360:372	arg1	AgNP-BNCP					381:389	AgNP-BNCP	381:389	AgNP-BNCP	381:389	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	2	18	theme	nanocellulose	360:372	arg1	paper					374:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper	319:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	0	19	theme	Silver	0:5	arg1	composites					50:59	Silver nanoparticle/bacterial nanocellulose paper composites	0:59	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.	0:126	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	2	20	theme	bacterial	350:358	arg1	AgNP-BNCP					381:389	AgNP-BNCP	381:389	AgNP-BNCP	381:389	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	2	20	theme	bacterial	350:358	arg1	paper					374:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper	319:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	2	21	theme	vacuum-assisted	420:434	arg1	filtration					436:445	vacuum-assisted filtration	420:445	vacuum-assisted filtration	420:445	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	0	22	theme	paper	44:48	arg1	composites					50:59	Silver nanoparticle/bacterial nanocellulose paper composites	0:59	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.	0:126	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	4	23	theme	4-aminothiophenol	782:798	arg1	detection					769:777	the detection	765:777	the detection of 4-aminothiophenol and methomyl pesticide	765:821	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	5	24	theme	simple	971:976	arg1	approach					1014:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	2	25	theme	nanoparticle-	336:348	arg1	AgNP-BNCP					381:389	AgNP-BNCP	381:389	AgNP-BNCP	381:389	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	2	25	theme	nanoparticle-	336:348	arg1	paper					374:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper	319:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	3	26	from	depth	627:631	arg1	substrate					640:648	the substrate	636:648	the substrate	636:648	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	5	27	theme	rapid	878:882	arg1	detection					884:892	a direct and rapid detection	865:892	a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs	865:936	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	0	28	theme	nanocellulose	30:42	arg1	composites					50:59	Silver nanoparticle/bacterial nanocellulose paper composites	0:59	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.	0:126	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	5	29	theme	effective	982:990	arg1	approach					1014:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	a simple and effective 'paste-and-read' SERS approach	969:1021	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	2	30	theme	silver	329:334	arg1	AgNP-BNCP					381:389	AgNP-BNCP	381:389	AgNP-BNCP	381:389	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	2	30	theme	silver	329:334	arg1	paper					374:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper	319:378	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	5	31	theme	direct	867:872	arg1	detection					884:892	a direct and rapid detection	865:892	a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs	865:936	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	5	32	theme	optical	835:841	arg1	transparency					843:854	the optical transparency	831:854	the optical transparency of BNCP	831:862	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	6	33	theme	in-situ	1102:1108	arg1	analysis					1115:1122	user-friendly in-situ SERS analysis	1088:1122	user-friendly in-situ SERS analysis	1088:1122	These results demonstrate potential of AgNP-BNCP composites for user-friendly in-situ SERS analysis.					
32122492	4	34	theme	good	711:714	arg1	reproducibility					716:730	good reproducibility	711:730	good reproducibility	711:730	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	4	35	theme	SERS	692:695	arg1	activity					697:704	high SERS activity	687:704	high SERS activity	687:704	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	3	36	theme	BNC	473:475	arg1	hydrogel					477:484	BNC hydrogel	473:484	BNC hydrogel	473:484	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	4	37	theme	fabricated	655:664	arg1	AgNP-BNCPs					666:675	The fabricated AgNP-BNCPs	651:675	The fabricated AgNP-BNCPs	651:675	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	5	38	theme	methomyl	897:904	arg1	detection					884:892	a direct and rapid detection	865:892	a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs	865:936	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	1	39	theme	in-situ	236:242	arg1	detection					244:252	in-situ detection	236:252	in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	236:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	0	40	theme	SERS	80:83	arg1	detection					85:93	paste-and-read SERS detection	65:93	paste-and-read SERS detection of pesticides on fruit surfaces	65:125	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	6	41	theme	user-friendly	1088:1100	arg1	analysis					1115:1122	user-friendly in-situ SERS analysis	1088:1122	user-friendly in-situ SERS analysis	1088:1122	These results demonstrate potential of AgNP-BNCP composites for user-friendly in-situ SERS analysis.					
32122492	0	42	from	detection	85:93	arg1	surfaces					118:125	fruit surfaces	112:125	fruit surfaces	112:125	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	3	43	theme	few-micron	616:625	arg1	depth					627:631	a few-micron depth	614:631	a few-micron depth on the substrate	614:648	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	3	44	theme	nanofibrous	531:541	arg1	BNCP					543:546	nanofibrous BNCP	531:546	nanofibrous BNCP	531:546	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	0	45	theme	paste-and-read	65:78	arg1	detection					85:93	paste-and-read SERS detection	65:93	paste-and-read SERS detection of pesticides on fruit surfaces	65:125	Silver nanoparticle/bacterial nanocellulose paper composites for paste-and-read SERS detection of pesticides on fruit surfaces.					
32122492	3	46	theme	ambient	553:559	arg1	process					568:574	ambient drying process	553:574	ambient drying process	553:574	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	3	47	theme	BNCP	543:546	arg1	network					520:526	the network	516:526	the network of nanofibrous BNCP upon ambient drying process	516:574	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	5	48	from	detection	884:892	arg1	peels					915:919	fruit peels	909:919	fruit peels using AgNP-BNCPs	909:936	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	3	49	theme	SERS	593:596	arg1	hotspots					598:605	3D SERS hotspots	590:605	3D SERS hotspots within a few-micron depth on the substrate	590:648	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	2	50	theme	paper	374:378	arg1	composites					392:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites	319:401	Plasmonic silver nanoparticle- bacterial nanocellulose paper (AgNP-BNCP) composites were prepared by vacuum-assisted filtration.					
32122492	6	51	theme	composites	1073:1082	arg1	potential					1050:1058	potential	1050:1058	potential of AgNP-BNCP composites for user-friendly in-situ SERS analysis	1050:1122	These results demonstrate potential of AgNP-BNCP composites for user-friendly in-situ SERS analysis.					
32122492	4	52	theme	high	687:690	arg1	activity					697:704	high SERS activity	687:704	high SERS activity	687:704	The fabricated AgNP-BNCPs exhibited high SERS activity with good reproducibility and stability as demonstrated by the detection of 4-aminothiophenol and methomyl pesticide.					
32122492	3	53	theme	drying	561:566	arg1	process					568:574	ambient drying process	553:574	ambient drying process	553:574	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	5	54	theme	fruit	909:913	arg1	peels					915:919	fruit peels	909:919	fruit peels using AgNP-BNCPs	909:936	Due to the optical transparency of BNCP, a direct and rapid detection of methomyl on fruit peels using AgNP-BNCPs can be achieved, demonstrating a simple and effective 'paste-and-read' SERS approach.					
32122492	1	55	theme	pesticides	257:266	arg1	detection					244:252	in-situ detection	236:252	in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	236:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
32122492	3	56	theme	loading	454:460	arg1	AgNPs					462:466	loading AgNPs	454:466	loading AgNPs into BNC hydrogel	454:484	After loading AgNPs into BNC hydrogel, AgNPs were trapped firmly in the network of nanofibrous BNCP upon ambient drying process, resulting in 3D SERS hotspots within a few-micron depth on the substrate.					
32122492	1	57	theme	eco-friendly	159:170	arg1	substrate					222:230	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate	156:230	an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC)	156:316	This study aimed to develop an eco-friendly flexible surface-enhanced Raman scattering (SERS) substrate for in-situ detection of pesticides using biodegradable bacterial nanocellulose (BNC).					
33170637	12	0	with	patients	2065:2072	arg1	DVT					2079:2081	DVT	2079:2081	DVT	2079:2081	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	12	0	with	patients	2065:2072	arg1	conditions					2105:2114	potentially other conditions	2087:2114	potentially other conditions	2087:2114	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	1	1	theme	acoustic	158:165	arg1	response					167:174	their unique acoustic response	145:174	their unique acoustic response	145:174	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	11	2	theme	phospholipid	1772:1783	arg1	composition					1785:1795	the phospholipid composition	1768:1795	the phospholipid composition	1768:1795	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	3	3	theme	pathophysiologic	494:509	arg1	changes					511:517	pathophysiologic changes	494:517	pathophysiologic changes	494:517	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	5	4	theme	decreased	945:953	arg1	oscillation					955:965	decreased oscillation	945:965	decreased oscillation	945:965	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	10	5	theme	harmonic	1620:1627	arg1	signal					1629:1634	harmonic signal	1620:1634	harmonic signal	1620:1634	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	6	6	theme	proof-of-concept	976:991	arg1	study					993:997	this proof-of-concept study	971:997	this proof-of-concept study	971:997	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	2	7	theme	surrounding	318:328	arg1	tissues					330:336	surrounding tissues	318:336	surrounding tissues	318:336	This unique response is derived from MBs' elasticity that allows them to oscillate differently from surrounding tissues.					
33170637	6	8	theme	activatable	1091:1101	arg1	platform					1127:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	9	9	theme	harmonic	1435:1442	arg1	signal					1444:1449	harmonic signal	1435:1449	harmonic signal	1435:1449	pH-MBs imaged before and after acidification increased harmonic signal fivefold.					
33170637	3	10	theme	main	349:352	arg1	use					354:356	the main use	345:356	the main use of MBs in the clinic	345:377	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	1	11	theme	ultrasound	110:119	arg1	agents					130:135	optimal ultrasound contrast agents	102:135	optimal ultrasound contrast agents	102:135	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	1	11	theme	ultrasound	110:119	arg1	Microbubbles					79:90	Microbubbles	79:90	Microbubbles (MBs)	79:96	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	6	12	theme	targeted	1078:1085	arg1	platform					1127:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	8	13	theme	Cadence	1345:1351	arg1	sequencing					1368:1377	Cadence contrast pulse sequencing	1345:1377	Cadence contrast pulse sequencing	1345:1377	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	11	14	theme	desired	1882:1888	arg1	response					1890:1897	the desired response	1878:1897	the desired response needed	1878:1904	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	3	15	theme	imparting	417:425	arg1	MBs					427:429	imparting MBs	417:429	imparting MBs with bioresponsive properties	417:459	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	7	16	theme	pH-MBs	1179:1184	arg1	Conjugation					1137:1147	Conjugation	1137:1147	Conjugation of HA to MBs	1137:1160	Conjugation of HA to MBs and targeting of pH-MBs to CD44-positive cells were validated.					
33170637	7	16	theme	pH-MBs	1179:1184	arg1	targeting					1166:1174	targeting	1166:1174	targeting of pH-MBs to CD44-positive cells	1166:1207	Conjugation of HA to MBs and targeting of pH-MBs to CD44-positive cells were validated.					
33170637	6	17	used	used	1003:1006	arg2	we					1000:1001	we	1000:1001	we	1000:1001	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	10	18	theme	cross-linker	1489:1500	arg1	cleavage					1473:1480	the cleavage	1469:1480	the cleavage of the cross-linker we used	1469:1508	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	10	18	theme	cross-linker	1489:1500	arg1	reversible					1513:1522	reversible	1513:1522	reversible	1513:1522	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	5	19	theme	increased	918:926	arg1	stiffness					931:939	increased MB stiffness	918:939	increased MB stiffness	918:939	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	8	20	theme	Harmonic	1225:1232	arg1	loss					1241:1244	Harmonic signal loss	1225:1244	Harmonic signal loss due to stiffening of pH-MBs' shell	1225:1279	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	12	21	theme	targeted	1962:1969	arg1	MBs					1987:1989	targeted and activatable MBs	1962:1989	targeted and activatable MBs	1962:1989	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	4	22	theme	vein	718:721	arg1	thrombosis					723:732	deep vein thrombosis	713:732	deep vein thrombosis (DVT)	713:738	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	4	22	theme	vein	718:721	arg1	DVT					735:737	DVT	735:737	DVT	735:737	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	1	23	theme	exquisite	187:195	arg1	sensitivity					197:207	exquisite sensitivity	187:207	exquisite sensitivity	187:207	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	5	24	theme	perfluorobutane-filled	779:800	arg1	MBs					802:804	perfluorobutane-filled MBs	779:804	perfluorobutane-filled MBs	779:804	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	4	25	theme	damping	544:550	arg1	oscillations					557:568	damping MBs' oscillations	544:568	damping MBs' oscillations	544:568	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	8	26	theme	signal	1234:1239	arg1	loss					1241:1244	Harmonic signal loss	1225:1244	Harmonic signal loss due to stiffening of pH-MBs' shell	1225:1279	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	4	27	theme	deep	713:716	arg1	thrombosis					723:732	deep vein thrombosis	713:732	deep vein thrombosis (DVT)	713:738	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	4	27	theme	deep	713:716	arg1	DVT					735:737	DVT	735:737	DVT	735:737	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	12	28	dep	diagnosis	2037:2045	arg1	the					2033:2035	the	2033:2035	the	2033:2035	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	4	29	theme	interest	647:654	arg1	biomarker					634:642	the biomarker	630:642	the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT)	630:738	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	8	30	theme	clinical	1303:1310	arg1	scanner					1323:1329	a clinical ultrasound scanner	1301:1329	a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing	1301:1377	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	5	31	theme	hyaluronic	811:820	arg1	HA					828:829	HA	828:829	HA	828:829	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	5	31	theme	hyaluronic	811:820	arg1	acid					822:825	hyaluronic acid	811:825	hyaluronic acid (HA)	811:830	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	5	32	theme	MB	928:929	arg1	stiffness					931:939	increased MB stiffness	918:939	increased MB stiffness	918:939	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	10	33	dep	cross-linker	1489:1500	arg1	used					1505:1508	used	1505:1508	used	1505:1508	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	1	34	theme	optimal	102:108	arg1	agents					130:135	optimal ultrasound contrast agents	102:135	optimal ultrasound contrast agents	102:135	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	1	34	theme	optimal	102:108	arg1	Microbubbles					79:90	Microbubbles	79:90	Microbubbles (MBs)	79:96	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	8	35	theme	pulse	1362:1366	arg1	sequencing					1368:1377	Cadence contrast pulse sequencing	1345:1377	Cadence contrast pulse sequencing	1345:1377	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	12	36	theme	patients	2065:2072	arg1	management					2051:2060	management	2051:2060	management	2051:2060	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	12	36	theme	patients	2065:2072	arg1	diagnosis					2037:2045	diagnosis	2037:2045	diagnosis	2037:2045	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	0	37	theme	Activatable	39:49	arg1	Agents					71:76	Activatable Ultrasound Contrast Agents	39:76	Activatable Ultrasound Contrast Agents	39:76	Microbubbles Cloaked with Hydrogels as Activatable Ultrasound Contrast Agents.					
33170637	12	38	theme	activatable	1975:1985	arg1	MBs					1987:1989	targeted and activatable MBs	1962:1989	targeted and activatable MBs	1962:1989	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	3	39	from	use	354:356	arg1	clinic					372:377	the clinic	368:377	the clinic	368:377	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	12	40	theme	other	2099:2103	arg1	conditions					2105:2114	potentially other conditions	2087:2114	potentially other conditions	2087:2114	In this study, we established the feasibility of using targeted and activatable MBs and plan to apply this platform to aid in the diagnosis and management of patients with DVT and potentially other conditions.					
33170637	3	41	theme	perfusion	398:406	arg1	imaging					408:414	perfusion imaging	398:414	perfusion imaging	398:414	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	7	42	theme	HA	1152:1153	arg1	Conjugation					1137:1147	Conjugation	1137:1147	Conjugation of HA to MBs	1137:1160	Conjugation of HA to MBs and targeting of pH-MBs to CD44-positive cells were validated.					
33170637	7	42	theme	HA	1152:1153	arg1	targeting					1166:1174	targeting	1166:1174	targeting of pH-MBs to CD44-positive cells	1166:1207	Conjugation of HA to MBs and targeting of pH-MBs to CD44-positive cells were validated.					
33170637	11	43	theme	rise	1685:1688	arg1	magnitude					1698:1706	the magnitude	1694:1706	the magnitude of harmonic signal increase	1694:1734	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	11	43	theme	rise	1685:1688	arg1	rate					1677:1680	The rate	1673:1680	The rate of rise	1673:1688	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	3	44	theme	bioresponsive	436:448	arg1	properties					450:459	bioresponsive properties	436:459	bioresponsive properties	436:459	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	0	45	theme	Contrast	62:69	arg1	Agents					71:76	Activatable Ultrasound Contrast Agents	39:76	Activatable Ultrasound Contrast Agents	39:76	Microbubbles Cloaked with Hydrogels as Activatable Ultrasound Contrast Agents.					
33170637	6	46	theme	pH-sensitive	1021:1032	arg1	cross-linker					1034:1045	a reversible pH-sensitive cross-linker	1008:1045	a reversible pH-sensitive cross-linker	1008:1045	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	10	47	link	cross-linked	1656:1667	arg1	HA					1669:1670	the cross-linked HA	1652:1670	the cross-linked HA	1652:1670	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	4	48	dep	they	615:618	arg1	encounter					620:628	encounter	620:628	encounter	620:628	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	8	49	theme	contrast	1353:1360	arg1	sequencing					1368:1377	Cadence contrast pulse sequencing	1345:1377	Cadence contrast pulse sequencing	1345:1377	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	4	50	theme	diseases	696:703	arg1	specificity					681:691	specificity	681:691	specificity	681:691	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	4	50	theme	diseases	696:703	arg1	detection					667:675	detection	667:675	detection	667:675	This can be achieved by damping MBs' oscillations to silence their signal and rescuing it when they encounter the biomarker of interest to improve detection and specificity of diseases such as deep vein thrombosis (DVT).					
33170637	0	51	theme	Ultrasound	51:60	arg1	Agents					71:76	Activatable Ultrasound Contrast Agents	39:76	Activatable Ultrasound Contrast Agents	39:76	Microbubbles Cloaked with Hydrogels as Activatable Ultrasound Contrast Agents.					
33170637	11	52	theme	HA	1815:1816	arg1	cross-linkers					1818:1830	HA cross-linkers	1815:1830	HA cross-linkers	1815:1830	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	10	53	theme	harmonic	1525:1532	arg1	signal					1534:1539	harmonic signal	1525:1539	harmonic signal	1525:1539	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	11	54	theme	signal	1720:1725	arg1	increase					1727:1734	harmonic signal increase	1711:1734	harmonic signal increase	1711:1734	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	2	55	theme	unique	223:228	arg1	response					230:237	This unique response	218:237	This unique response	218:237	This unique response is derived from MBs' elasticity that allows them to oscillate differently from surrounding tissues.					
33170637	7	56	theme	CD44-positive	1189:1201	arg1	cells					1203:1207	CD44-positive cells	1189:1207	CD44-positive cells	1189:1207	Conjugation of HA to MBs and targeting of pH-MBs to CD44-positive cells were validated.					
33170637	1	57	theme	contrast	121:128	arg1	agents					130:135	optimal ultrasound contrast agents	102:135	optimal ultrasound contrast agents	102:135	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	1	57	theme	contrast	121:128	arg1	Microbubbles					79:90	Microbubbles	79:90	Microbubbles (MBs)	79:96	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	11	58	theme	cross-linkers	1818:1830	arg1	number					1805:1810	the number	1801:1810	the number of HA cross-linkers	1801:1830	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	11	58	theme	cross-linkers	1818:1830	arg1	composition					1785:1795	the phospholipid composition	1768:1795	the phospholipid composition	1768:1795	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	11	59	theme	increase	1727:1734	arg1	magnitude					1698:1706	the magnitude	1694:1706	the magnitude of harmonic signal increase	1694:1734	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	11	59	theme	increase	1727:1734	arg1	rate					1677:1680	The rate	1673:1680	The rate of rise	1673:1688	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	11	60	theme	harmonic	1711:1718	arg1	increase					1727:1734	harmonic signal increase	1711:1734	harmonic signal increase	1711:1734	The rate of rise and the magnitude of harmonic signal increase could be manipulated by varying the phospholipid composition and the number of HA cross-linkers, indicating that the platform can be tuned to the desired response needed.					
33170637	6	61	theme	pH-MB	1120:1124	arg1	platform					1127:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	6	62	theme	reversible	1010:1019	arg1	cross-linker					1034:1045	a reversible pH-sensitive cross-linker	1008:1045	a reversible pH-sensitive cross-linker	1008:1045	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	3	63	with	MBs	427:429	arg1	properties					450:459	bioresponsive properties	436:459	bioresponsive properties	436:459	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
33170637	5	64	theme	harmonic	891:898	arg1	signal					900:905	harmonic signal	891:905	harmonic signal	891:905	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	5	65	theme	biodegradable	858:870	arg1	linkers					872:878	biodegradable linkers	858:878	biodegradable linkers	858:878	Here, we demonstrate that conjugating perfluorobutane-filled MBs with hyaluronic acid (HA) and cross-linking HA with biodegradable linkers eliminates harmonic signal because of increased MB stiffness and decreased oscillation.					
33170637	8	66	theme	ultrasound	1312:1321	arg1	scanner					1323:1329	a clinical ultrasound scanner	1301:1329	a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing	1301:1377	Harmonic signal loss due to stiffening of pH-MBs' shell was confirmed using a clinical ultrasound scanner equipped with Cadence contrast pulse sequencing.					
33170637	6	67	theme	pH-sensitive	1103:1114	arg1	platform					1127:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	6	68	theme	MB	1116:1117	arg1	platform					1127:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	this targeted and activatable pH-sensitive MB (pH-MB) platform	1073:1134	In this proof-of-concept study, we used a reversible pH-sensitive cross-linker to establish and validate this targeted and activatable pH-sensitive MB (pH-MB) platform.					
33170637	10	69	theme	cross-linked	1656:1667	arg1	HA					1669:1670	the cross-linked HA	1652:1670	the cross-linked HA	1652:1670	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	10	70	used	used	1505:1508	arg2	we					1502:1503	we	1502:1503	we	1502:1503	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	10	71	theme	acidic	1569:1574	arg1	suspension					1576:1585	the acidic suspension	1565:1585	the acidic suspension	1565:1585	Because the cleavage of the cross-linker we used is reversible, harmonic signal was silenced again when the acidic suspension was neutralized, confirming that harmonic signal is dependent on the cross-linked HA.					
33170637	1	72	theme	unique	151:156	arg1	response					167:174	their unique acoustic response	145:174	their unique acoustic response	145:174	Microbubbles (MBs) are optimal ultrasound contrast agents because their unique acoustic response allows for exquisite sensitivity in vivo.					
33170637	3	73	theme	MBs	361:363	arg1	use					354:356	the main use	345:356	the main use of MBs in the clinic	345:377	While the main use of MBs in the clinic is for cardiac and perfusion imaging, imparting MBs with bioresponsive properties would expand their use to detect pathophysiologic changes.					
32337732	5	0	theme	property	668:675	arg1	analysis					677:684	physical property analysis	659:684	physical property analysis	659:684	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	4	1	theme	diffraction	561:571	arg1	analysis					573:580	X-ray diffraction analysis	555:580	X-ray diffraction analysis	555:580	X-ray diffraction analysis confirmed structure destruction of starch pellets.					
32337732	7	2	theme	gelatinization	1016:1029	arg1	viscosity					1074:1082	trough viscosity	1067:1082	trough viscosity	1067:1082	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	2	theme	gelatinization	1016:1029	arg1	viscosity					1056:1064	peak viscosity	1051:1064	peak viscosity	1051:1064	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	2	theme	gelatinization	1016:1029	arg1	properties					1031:1040	gelatinization properties	1016:1040	gelatinization properties	1016:1040	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	2	theme	gelatinization	1016:1029	arg1	viscosity					1095:1103	breakdown viscosity	1085:1103	breakdown viscosity	1085:1103	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	2	theme	gelatinization	1016:1029	arg1	viscosity					1116:1124	final viscosity	1110:1124	final viscosity	1110:1124	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	6	3	theme	solubility	774:783	arg1	index					785:789	the water solubility index	764:789	the water solubility index	764:789	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	2	4	theme	processed	250:258	arg1	powders					267:273	ball-mill processed potato powders	240:273	ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm)	240:330	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	10	5	theme	products	1589:1596	arg1	development					1561:1571	the development	1557:1571	the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties	1557:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	10	6	theme	ethnic	1631:1636	arg1	products					1643:1650	ethnic food products	1631:1650	ethnic food products	1631:1650	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	2	7	theme	different	280:288	arg1	sizes					299:303	different particle sizes	280:303	different particle sizes (278, 208, 129, and 62 μm)	280:330	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	6	8	theme	redness	904:910	arg1	values					917:922	the brightness (L*) and redness (b*) values	880:922	values	917:922	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	6	8	theme	redness	904:910	arg1	*					914:914	b*	913:914	b*	913:914	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	4	9	theme	starch	617:622	arg1	pellets					624:630	starch pellets	617:630	starch pellets	617:630	X-ray diffraction analysis confirmed structure destruction of starch pellets.					
32337732	1	10	theme	BACKGROUND	86:95	arg1	source					119:124	a rich source	112:124	a rich source of high-quality protein and starch	112:159	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	1	10	theme	BACKGROUND	86:95	arg1	powder					104:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	10	11	theme	particular	1657:1666	arg1	properties					1695:1704	particular functional and rheological properties	1657:1704	particular functional and rheological properties	1657:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	8	12	theme	Oscillatory	1127:1137	arg1	results					1148:1154	Oscillatory rheology results	1127:1154	Oscillatory rheology results	1127:1154	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	6	13	theme	L	896:896	arg1	brightness					884:893	the brightness (L*) and redness (b*) values	880:922	brightness	884:893	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	6	13	theme	L	896:896	arg1	*					897:897	L*	896:897	L*	896:897	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	9	14	theme	potato	1407:1412	arg1	powder					1414:1419	the 129 μm potato powder	1396:1419	the 129 μm potato powder	1396:1419	The hydrolysis rate and glycemic index also increased in the 129 μm potato powder.					
32337732	10	15	theme	gluten-free	1606:1616	arg1	products					1618:1625	gluten-free products	1606:1625	gluten-free products	1606:1625	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	6	16	theme	potato	931:936	arg1	powders					938:944	the potato powders	927:944	the potato powders	927:944	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	0	17	theme	granule	11:17	arg1	size					19:22	granule size	11:22	granule size	11:22	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	1	18	theme	high-quality	129:140	arg1	protein					142:148	high-quality protein	129:148	high-quality protein	129:148	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	8	19	located	observed	1301:1308	arg2	modulus					1288:1294	highest storage modulus	1272:1294	highest storage modulus (G') observed in the 129 μm particle size	1272:1336	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	19	located	observed	1301:1308	arg1	size					1333:1336	the 129 μm particle size	1313:1336	the 129 μm particle size	1313:1336	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	19	located	observed	1301:1308	arg2	G					1297:1297	G'	1297:1298	G'	1297:1298	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	20	theme	particle	1324:1331	arg1	size					1333:1336	the 129 μm particle size	1313:1336	the 129 μm particle size	1313:1336	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	0	21	theme	potato	71:76	arg1	powder					78:83	potato powder	71:83	potato powder	71:83	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	5	22	theme	physical	659:666	arg1	analysis					677:684	physical property analysis	659:684	physical property analysis	659:684	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	2	23	with	powders	267:273	arg1	sizes					299:303	different particle sizes	280:303	different particle sizes (278, 208, 129, and 62 μm)	280:330	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	0	24	from	Effects	0:6	arg1	properties					57:66	physicochemical and digestive properties	27:66	physicochemical and digestive properties of potato powder	27:83	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	7	25	theme	final	1110:1114	arg1	viscosity					1116:1124	final viscosity	1110:1124	final viscosity	1110:1124	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	6	26	theme	b	913:913	arg1	values					917:922	the brightness (L*) and redness (b*) values	880:922	values	917:922	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	6	26	theme	b	913:913	arg1	*					914:914	b*	913:914	b*	913:914	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	7	27	theme	peak	1051:1054	arg1	viscosity					1056:1064	peak viscosity	1051:1064	peak viscosity	1051:1064	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	8	28	theme	loss	1239:1242	arg1	G″					1253:1254	G″	1253:1254	G″	1253:1254	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	28	theme	loss	1239:1242	arg1	modulus					1244:1250	loss modulus	1239:1250	loss modulus (G″)	1239:1255	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	9	29	theme	129 μm	1400:1405	arg1	powder					1414:1419	the 129 μm potato powder	1396:1419	the 129 μm potato powder	1396:1419	The hydrolysis rate and glycemic index also increased in the 129 μm potato powder.					
32337732	7	30	theme	trough	1067:1072	arg1	viscosity					1074:1082	trough viscosity	1067:1082	trough viscosity	1067:1082	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	3	31	theme	diffraction	470:480	arg1	analysis					482:489	laser diffraction analysis	464:489	laser diffraction analysis	464:489	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	2	32	theme	digestive	401:409	arg1	properties					411:420	physicochemical, pasting, rheological, and digestive properties	358:420	physicochemical, pasting, rheological, and digestive properties	358:420	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	8	33	theme	highest	1272:1278	arg1	G					1297:1297	G'	1297:1298	G'	1297:1298	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	33	theme	highest	1272:1278	arg1	modulus					1288:1294	highest storage modulus	1272:1294	highest storage modulus (G') observed in the 129 μm particle size	1272:1336	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	10	34	theme	grain	1542:1546	arg1	sizes					1548:1552	specific grain sizes	1533:1552	specific grain sizes	1533:1552	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	6	35	theme	power	804:808	arg1	values					810:815	power values	804:815	power values	804:815	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	11	36	theme	Chemical	1725:1732	arg1	Industry					1734:1741	Chemical Industry	1725:1741	Chemical Industry	1725:1741	© 2020 Society of Chemical Industry.					
32337732	10	37	theme	potato-based	1576:1587	arg1	products					1589:1596	potato-based products	1576:1596	potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties	1576:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	10	37	theme	potato-based	1576:1587	arg1	products					1643:1650	ethnic food products	1631:1650	ethnic food products	1631:1650	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	10	37	theme	potato-based	1576:1587	arg1	products					1618:1625	gluten-free products	1606:1625	gluten-free products	1606:1625	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	1	38	theme	functional	207:216	arg1	foods					218:222	functional foods	207:222	functional foods	207:222	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	4	39	theme	structure	592:600	arg1	destruction					602:612	structure destruction	592:612	structure destruction of starch pellets	592:630	X-ray diffraction analysis confirmed structure destruction of starch pellets.					
32337732	5	40	theme	starch	737:742	arg1	content					744:750	starch content	737:750	starch content	737:750	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	5	41	theme	Proximate	633:641	arg1	composition					643:653	Proximate composition	633:653	Proximate composition	633:653	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	3	42	theme	electron	440:447	arg1	microscopy					449:458	Scanning electron microscopy	431:458	Scanning electron microscopy	431:458	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	2	43	theme	potato	260:265	arg1	powders					267:273	ball-mill processed potato powders	240:273	ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm)	240:330	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	5	44	from	increase	696:703	arg1	water					712:716	the water	708:716	the water	708:716	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	5	44	from	increase	696:703	arg1	protein					724:730	protein	724:730	protein	724:730	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	5	44	from	increase	696:703	arg1	ash					719:721	ash	719:721	ash	719:721	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	5	44	from	increase	696:703	arg1	content					744:750	starch content	737:750	starch content	737:750	Proximate composition and physical property analysis showed an increase in the water, ash, protein, and starch content.					
32337732	2	45	theme	particle	290:297	arg1	sizes					299:303	different particle sizes	280:303	different particle sizes (278, 208, 129, and 62 μm)	280:330	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	6	46	dep	Meanwhile	753:761	arg1	values					810:815	power values	804:815	power values	804:815	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	4	47	theme	X-ray	555:559	arg1	analysis					573:580	X-ray diffraction analysis	555:580	X-ray diffraction analysis	555:580	X-ray diffraction analysis confirmed structure destruction of starch pellets.					
32337732	10	48	dep	CONCLUSION	1422:1431	arg1	provide					1445:1451	provide	1445:1451	provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties	1445:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	6	49	theme	water	768:772	arg1	index					785:789	the water solubility index	764:789	the water solubility index	764:789	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	2	50	theme	ball-mill	240:248	arg1	powders					267:273	ball-mill processed potato powders	240:273	ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm)	240:330	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	1	51	theme	starch	154:159	arg1	source					119:124	a rich source	112:124	a rich source of high-quality protein and starch	112:159	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	1	51	theme	starch	154:159	arg1	powder					104:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	6	52	theme	grain	856:860	arg1	size					862:865	grain size	856:865	grain size	856:865	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	8	53	theme	rheology	1139:1146	arg1	results					1148:1154	Oscillatory rheology results	1127:1154	Oscillatory rheology results	1127:1154	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	7	54	theme	large	985:989	arg1	changes					991:997	large changes	985:997	large changes	985:997	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	3	55	theme	mono-model	515:524	arg1	distributions					540:552	mono-model particle-size distributions	515:552	mono-model particle-size distributions	515:552	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	3	56	theme	samples	498:504	arg1	microscopy					449:458	Scanning electron microscopy	431:458	Scanning electron microscopy	431:458	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	3	56	theme	samples	498:504	arg1	analysis					482:489	laser diffraction analysis	464:489	laser diffraction analysis	464:489	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	1	57	theme	important	171:179	arg1	role					181:184	an important role	168:184	an important role	168:184	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	3	58	theme	particle-size	526:538	arg1	distributions					540:552	mono-model particle-size distributions	515:552	mono-model particle-size distributions	515:552	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	9	59	theme	glycemic	1363:1370	arg1	index					1372:1376	glycemic index	1363:1376	glycemic index	1363:1376	The hydrolysis rate and glycemic index also increased in the 129 μm potato powder.					
32337732	10	60	theme	functional	1668:1677	arg1	properties					1695:1704	particular functional and rheological properties	1657:1704	particular functional and rheological properties	1657:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	10	61	with	products	1618:1625	arg1	properties					1695:1704	particular functional and rheological properties	1657:1704	particular functional and rheological properties	1657:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	4	62	theme	pellets	624:630	arg1	destruction					602:612	structure destruction	592:612	structure destruction of starch pellets	592:630	X-ray diffraction analysis confirmed structure destruction of starch pellets.					
32337732	7	63	theme	particle	952:959	arg1	size					961:964	particle size	952:964	particle size reduced to 129 μm	952:982	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	10	64	with	products	1643:1650	arg1	properties					1695:1704	particular functional and rheological properties	1657:1704	particular functional and rheological properties	1657:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	7	65	located	observed	1004:1011	arg1	properties					1031:1040	gelatinization properties	1016:1040	gelatinization properties	1016:1040	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	65	located	observed	1004:1011	arg1	viscosity					1074:1082	trough viscosity	1067:1082	trough viscosity	1067:1082	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	65	located	observed	1004:1011	arg1	viscosity					1116:1124	final viscosity	1110:1124	final viscosity	1110:1124	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	65	located	observed	1004:1011	arg1	viscosity					1056:1064	peak viscosity	1051:1064	peak viscosity	1051:1064	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	65	located	observed	1004:1011	arg1	viscosity					1095:1103	breakdown viscosity	1085:1103	breakdown viscosity	1085:1103	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	7	65	located	observed	1004:1011	arg2	changes					991:997	large changes	985:997	large changes	985:997	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	1	66	theme	rich	114:117	arg1	source					119:124	a rich source	112:124	a rich source of high-quality protein and starch	112:159	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	1	66	theme	rich	114:117	arg1	powder					104:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	6	67	theme	powders	938:944	arg1	*					897:897	L*	896:897	L*	896:897	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	6	67	theme	powders	938:944	arg1	brightness					884:893	the brightness (L*) and redness (b*) values	880:922	brightness	884:893	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	6	67	theme	powders	938:944	arg1	values					917:922	the brightness (L*) and redness (b*) values	880:922	values	917:922	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	6	67	theme	powders	938:944	arg1	*					914:914	b*	913:914	b*	913:914	Meanwhile, the water solubility index and swelling power values were found to increase with decreasing grain size, and so were the brightness (L*) and redness (b*) values of the potato powders.					
32337732	8	68	theme	129 μm	1317:1322	arg1	size					1333:1336	the 129 μm particle size	1313:1336	the 129 μm particle size	1313:1336	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	69	theme	particle	1195:1202	arg1	size					1204:1207	particle size	1195:1207	particle size	1195:1207	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	10	70	theme	rheological	1683:1693	arg1	properties					1695:1704	particular functional and rheological properties	1657:1704	particular functional and rheological properties	1657:1704	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	9	71	theme	hydrolysis	1343:1352	arg1	rate					1354:1357	The hydrolysis rate	1339:1357	The hydrolysis rate	1339:1357	The hydrolysis rate and glycemic index also increased in the 129 μm potato powder.					
32337732	0	72	theme	powder	78:83	arg1	properties					57:66	physicochemical and digestive properties	27:66	physicochemical and digestive properties of potato powder	27:83	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	1	73	theme	protein	142:148	arg1	source					119:124	a rich source	112:124	a rich source of high-quality protein and starch	112:159	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	1	73	theme	protein	142:148	arg1	powder					104:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	2	74	theme	properties	411:420	arg1	terms					349:353	terms	349:353	terms of physicochemical, pasting, rheological, and digestive properties	349:420	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	8	75	theme	storage	1214:1220	arg1	G					1231:1231	G'	1231:1232	G'	1231:1232	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	75	theme	storage	1214:1220	arg1	modulus					1222:1228	the storage modulus	1210:1228	the storage modulus (G')	1210:1233	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	10	76	theme	food	1638:1641	arg1	products					1643:1650	ethnic food products	1631:1650	ethnic food products	1631:1650	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	7	77	theme	breakdown	1085:1093	arg1	viscosity					1095:1103	breakdown viscosity	1085:1103	breakdown viscosity	1085:1103	With particle size reduced to 129 μm, large changes were observed in gelatinization properties, such as peak viscosity, trough viscosity, breakdown viscosity, and final viscosity.					
32337732	8	78	from	decrease	1183:1190	arg1	size					1204:1207	particle size	1195:1207	particle size	1195:1207	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	3	79	dep	RESULTS	423:429	arg1	revealed					506:513	revealed	506:513	revealed mono-model particle-size distributions	506:552	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	2	80	theme	pasting	375:381	arg1	properties					411:420	physicochemical, pasting, rheological, and digestive properties	358:420	physicochemical, pasting, rheological, and digestive properties	358:420	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	2	81	dep	sizes	299:303	arg1	129					316:318	129	316:318	129	316:318	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	2	81	dep	sizes	299:303	arg1	62 μm					325:329	62 μm	325:329	62 μm	325:329	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	0	82	theme	size	19:22	arg1	Effects					0:6	Effects	0:6	Effects of granule size on physicochemical and digestive properties of potato powder	0:83	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	1	83	theme	Potato	97:102	arg1	source					119:124	a rich source	112:124	a rich source of high-quality protein and starch	112:159	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	1	83	theme	Potato	97:102	arg1	powder					104:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder	86:109	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32337732	2	84	theme	physicochemical	358:372	arg1	properties					411:420	physicochemical, pasting, rheological, and digestive properties	358:420	physicochemical, pasting, rheological, and digestive properties	358:420	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	0	85	theme	physicochemical	27:41	arg1	properties					57:66	physicochemical and digestive properties	27:66	physicochemical and digestive properties of potato powder	27:83	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	10	86	theme	quality	1500:1506	arg1	characteristics					1508:1522	quality characteristics	1500:1522	quality characteristics	1500:1522	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	0	87	theme	digestive	47:55	arg1	properties					57:66	physicochemical and digestive properties	27:66	physicochemical and digestive properties of potato powder	27:83	Effects of granule size on physicochemical and digestive properties of potato powder.					
32337732	2	88	theme	rheological	384:394	arg1	properties					411:420	physicochemical, pasting, rheological, and digestive properties	358:420	physicochemical, pasting, rheological, and digestive properties	358:420	In this study, ball-mill processed potato powders with different particle sizes (278, 208, 129, and 62 μm) were analyzed in terms of physicochemical, pasting, rheological, and digestive properties.					
32337732	3	89	theme	Scanning	431:438	arg1	microscopy					449:458	Scanning electron microscopy	431:458	Scanning electron microscopy	431:458	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	10	90	theme	specific	1533:1540	arg1	sizes					1548:1552	specific grain sizes	1533:1552	specific grain sizes	1533:1552	CONCLUSION The results provide information that could be useful for improving quality characteristics by using specific grain sizes in the development of potato-based products such as gluten-free products and ethnic food products with particular functional and rheological properties.					
32337732	8	91	theme	storage	1280:1286	arg1	G					1297:1297	G'	1297:1298	G'	1297:1298	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	8	91	theme	storage	1280:1286	arg1	modulus					1288:1294	highest storage modulus	1272:1294	highest storage modulus (G') observed in the 129 μm particle size	1272:1336	Oscillatory rheology results also showed that, with the decrease in particle size, the storage modulus (G') and loss modulus (G″) improved, with highest storage modulus (G') observed in the 129 μm particle size.					
32337732	3	92	theme	laser	464:468	arg1	analysis					482:489	laser diffraction analysis	464:489	laser diffraction analysis	464:489	RESULTS Scanning electron microscopy and laser diffraction analysis of the samples revealed mono-model particle-size distributions.					
32337732	1	93	theme	foods	218:222	arg1	production					193:202	the production	189:202	the production of functional foods	189:222	BACKGROUND Potato powder, a rich source of high-quality protein and starch, plays an important role in the production of functional foods.					
32069712	4	0	theme	scission	903:910	arg1	mechanism					912:920	chain scission mechanism	897:920	chain scission mechanism leading to the formation of intermediates	897:962	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	6	1	theme	rough	1190:1194	arg1	patches					1196:1202	rough patches	1190:1202	rough patches on the PCL surface	1190:1221	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	2	2	theme	simulated	527:535	arg1	conditions					548:557	simulated composting conditions	527:557	simulated composting conditions	527:557	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	5	3	theme	biopolymeric	1047:1058	arg1	surfaces					1060:1067	the blended biopolymeric surfaces	1035:1067	the blended biopolymeric surfaces	1035:1067	Analytical techniques revealed the formation of microbial biofilms on the blended biopolymeric surfaces.					
32069712	4	4	theme	weight	825:830	arg1	analysis					832:839	Molecular weight analysis	815:839	Molecular weight analysis of the samples	815:854	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	1	5	theme	poly	229:232	arg1	polyblends					215:224	the polyblends	211:224	the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions	211:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	4	6	theme	chain	897:901	arg1	mechanism					912:920	chain scission mechanism	897:920	chain scission mechanism leading to the formation of intermediates	897:962	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	1	7	theme	microcrystalline	281:296	arg1	MCC					309:311	MCC	309:311	MCC	309:311	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	7	theme	microcrystalline	281:296	arg1	cellulose					298:306	microcrystalline cellulose	281:306	microcrystalline cellulose (MCC)	281:312	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	0	8	theme	composting	142:151	arg1	conditions					153:162	composting conditions	142:162	composting conditions	142:162	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	2	9	theme	D5338-15	601:608	arg1	protocol					610:617	the standard ASTM International D5338-15 protocol	569:617	the standard ASTM International D5338-15 protocol	569:617	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	3	10	with	blends	637:642	arg1	concentration					658:670	a higher concentration	649:670	a higher concentration of PCL and MCC in the PLA matrix	649:703	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	0	11	theme	/Polycaprolactone	68:84	arg1	polyblends					125:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	1	12	theme	cellulose	298:306	arg1	polyblends					215:224	the polyblends	211:224	the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions	211:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	2	13	theme	polyblends	510:519	arg1	fate					498:501	the environmental fate	480:501	the environmental fate of the polyblends	480:519	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	1	14	theme	blends	382:387	arg1	properties					362:371	the properties	358:371	the properties of those blends with that of neat PLA and neat PCL	358:422	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	6	15	theme	fibril-like	1138:1148	arg1	structures					1150:1159	fibril-like structures	1138:1159	fibril-like structures	1138:1159	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	3	16	theme	MCC	799:801	arg1	components					803:812	MCC components	799:812	MCC components	799:812	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	1	17	theme	lactic	234:239	arg1	poly					229:232	poly	229:232	poly(lactic acid) (PLA)	229:251	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	17	theme	lactic	234:239	arg1	acid					241:244	lactic acid	234:244	lactic acid	234:244	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	18	from	comparison	344:353	arg1	compositions					327:338	different compositions	317:338	different compositions	317:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	6	19	theme	PCL	1211:1213	arg1	surface					1215:1221	the PCL surface	1207:1221	the PCL surface	1207:1221	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	4	20	from	decrease	865:872	arg1	weight					883:888	their weight	877:888	their weight due to chain scission mechanism leading to the formation of intermediates	877:962	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	0	21	theme	PCL	87:89	arg1	polyblends					125:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	3	22	contain	having	780:785	arg1	blends					773:778	the blends	769:778	the blends having low PCL and MCC components	769:812	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	3	22	contain	having	780:785	arg2	components					803:812	MCC components	799:812	MCC components	799:812	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	3	22	contain	having	780:785	arg2	PCL					791:793	low PCL	787:793	low PCL	787:793	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	2	23	theme	standard	573:580	arg1	protocol					610:617	the standard ASTM International D5338-15 protocol	569:617	the standard ASTM International D5338-15 protocol	569:617	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	6	24	theme	samples	1258:1264	arg1	biodegradation					1236:1249	biodegradation	1236:1249	biodegradation of the samples	1236:1264	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	1	25	from	compositions	327:338	arg1	comparison					344:353	comparison	344:353	comparison of the properties of those blends with that of neat PLA and neat PCL	344:422	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	25	from	compositions	327:338	arg1	biodegradation					193:206	biodegradation	193:206	biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions	193:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	5	26	theme	blended	1039:1045	arg1	surfaces					1060:1067	the blended biopolymeric surfaces	1035:1067	the blended biopolymeric surfaces	1035:1067	Analytical techniques revealed the formation of microbial biofilms on the blended biopolymeric surfaces.					
32069712	6	27	from	patches	1196:1202	arg1	surface					1215:1221	the PCL surface	1207:1221	the PCL surface	1207:1221	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	7	28	theme	samples	1413:1419	arg1	biodegradability					1381:1396	the biodegradability	1377:1396	the biodegradability of the studied samples	1377:1419	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	5	29	from	formation	1000:1008	arg1	surfaces					1060:1067	the blended biopolymeric surfaces	1035:1067	the blended biopolymeric surfaces	1035:1067	Analytical techniques revealed the formation of microbial biofilms on the blended biopolymeric surfaces.					
32069712	7	30	theme	polyblends	1346:1355	arg1	characterization					1308:1323	the material characterization	1295:1323	the material characterization of PLA/PCL/MCC based polyblends	1295:1355	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	3	31	theme	higher	651:656	arg1	concentration					658:670	a higher concentration	649:670	a higher concentration of PCL and MCC in the PLA matrix	649:703	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	6	32	theme	patches	1196:1202	arg1	formation					1177:1185	the formation	1173:1185	the formation of rough patches on the PCL surface	1173:1221	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	1	33	theme	neat	402:405	arg1	PLA					407:409	neat PLA	402:409	neat PLA	402:409	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	4	34	theme	Molecular	815:823	arg1	analysis					832:839	Molecular weight analysis	815:839	Molecular weight analysis of the samples	815:854	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	0	35	theme	End-of-life	0:10	arg1	evaluation					12:21	End-of-life evaluation	0:21	End-of-life evaluation	0:21	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	3	36	theme	MCC	683:685	arg1	concentration					658:670	a higher concentration	649:670	a higher concentration of PCL and MCC in the PLA matrix	649:703	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	2	37	theme	composting	537:546	arg1	conditions					548:557	simulated composting conditions	527:557	simulated composting conditions	527:557	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	7	38	theme	studied	1405:1411	arg1	samples					1413:1419	the studied samples	1401:1419	the studied samples	1401:1419	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	3	39	from	concentration	658:670	arg1	matrix					698:703	the PLA matrix	690:703	the PLA matrix	690:703	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	0	40	theme	/Microcrystalline	91:107	arg1	polyblends					125:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	6	41	theme	electron	1094:1101	arg1	microscopy					1103:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	1	42	theme	present	169:175	arg1	study					177:181	The present study	165:181	The present study	165:181	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	4	43	theme	intermediates	950:962	arg1	formation					937:945	the formation	933:945	the formation of intermediates	933:962	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	3	44	theme	PCL	675:677	arg1	concentration					658:670	a higher concentration	649:670	a higher concentration of PCL and MCC in the PLA matrix	649:703	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	1	45	theme	different	317:325	arg1	compositions					327:338	different compositions	317:338	different compositions	317:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	6	46	theme	scanning	1085:1092	arg1	microscopy					1103:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	0	47	theme	Poly	45:48	arg1	polyblends					125:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	7	48	theme	based	1340:1344	arg1	polyblends					1346:1355	PLA/PCL/MCC based polyblends	1328:1355	PLA/PCL/MCC based polyblends	1328:1355	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	0	49	theme	cellulose	109:117	arg1	polyblends					125:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	5	50	theme	microbial	1013:1021	arg1	biofilms					1023:1030	microbial biofilms	1013:1030	microbial biofilms	1013:1030	Analytical techniques revealed the formation of microbial biofilms on the blended biopolymeric surfaces.					
32069712	3	51	theme	higher	712:717	arg1	percentage					741:750	higher carbon mineralization percentage	712:750	higher carbon mineralization percentage	712:750	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	1	52	theme	neat	415:418	arg1	PCL					420:422	neat PCL	415:422	neat PCL	415:422	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	3	53	theme	PLA	694:696	arg1	matrix					698:703	the PLA matrix	690:703	the PLA matrix	690:703	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	0	54	theme	lactic	50:55	arg1	Poly					45:48	Poly	45:48	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	0	54	theme	lactic	50:55	arg1	acid					57:60	lactic acid	50:60	lactic acid	50:60	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	1	55	theme	polycaprolactone	254:269	arg1	polyblends					215:224	the polyblends	211:224	the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions	211:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	56	from	polyblends	215:224	arg1	compositions					327:338	different compositions	317:338	different compositions	317:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	5	57	theme	biofilms	1023:1030	arg1	formation					1000:1008	the formation	996:1008	the formation of microbial biofilms on the blended biopolymeric surfaces	996:1067	Analytical techniques revealed the formation of microbial biofilms on the blended biopolymeric surfaces.					
32069712	1	58	from	biodegradation	193:206	arg1	compositions					327:338	different compositions	317:338	different compositions	317:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	6	59	theme	emission	1076:1083	arg1	microscopy					1103:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	0	60	theme	MCC	120:122	arg1	polyblends					125:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends	45:134	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	5	61	theme	Analytical	965:974	arg1	techniques					976:985	Analytical techniques	965:985	Analytical techniques	965:985	Analytical techniques revealed the formation of microbial biofilms on the blended biopolymeric surfaces.					
32069712	7	62	theme	PLA/PCL/MCC	1328:1338	arg1	polyblends					1346:1355	PLA/PCL/MCC based polyblends	1328:1355	PLA/PCL/MCC based polyblends	1328:1355	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	6	63	theme	Field	1070:1074	arg1	microscopy					1103:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy	1070:1112	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	3	64	theme	low	787:789	arg1	PCL					791:793	low PCL	787:793	low PCL	787:793	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	1	65	with	blends	382:387	arg1	that					394:397	that	394:397	that	394:397	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	2	66	theme	environmental	484:496	arg1	fate					498:501	the environmental fate	480:501	the environmental fate of the polyblends	480:519	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	7	67	theme	material	1299:1306	arg1	characterization					1308:1323	the material characterization	1295:1323	the material characterization of PLA/PCL/MCC based polyblends	1295:1355	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	2	68	theme	International	587:599	arg1	protocol					610:617	the standard ASTM International D5338-15 protocol	569:617	the standard ASTM International D5338-15 protocol	569:617	The samples were melt extruded and blended to evaluate the environmental fate of the polyblends under simulated composting conditions following the standard ASTM International D5338-15 protocol.					
32069712	3	69	theme	mineralization	726:739	arg1	percentage					741:750	higher carbon mineralization percentage	712:750	higher carbon mineralization percentage	712:750	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
32069712	0	70	theme	polyblends	125:134	arg1	biodegradation					27:40	biodegradation	27:40	biodegradation	27:40	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	0	70	theme	polyblends	125:134	arg1	evaluation					12:21	End-of-life evaluation	0:21	End-of-life evaluation	0:21	End-of-life evaluation and biodegradation of Poly(lactic acid) (PLA)/Polycaprolactone (PCL)/Microcrystalline cellulose (MCC) polyblends under composting conditions.					
32069712	1	71	theme	properties	362:371	arg1	comparison					344:353	comparison	344:353	comparison of the properties of those blends with that of neat PLA and neat PCL	344:422	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	71	theme	properties	362:371	arg1	biodegradation					193:206	biodegradation	193:206	biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions	193:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	4	72	theme	samples	848:854	arg1	analysis					832:839	Molecular weight analysis	815:839	Molecular weight analysis of the samples	815:854	Molecular weight analysis of the samples showed a decrease in their weight due to chain scission mechanism leading to the formation of intermediates.					
32069712	6	73	from	formation	1177:1185	arg1	surface					1215:1221	the PCL surface	1207:1221	the PCL surface	1207:1221	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	1	74	theme	polyblends	215:224	arg1	comparison					344:353	comparison	344:353	comparison of the properties of those blends with that of neat PLA and neat PCL	344:422	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	1	74	theme	polyblends	215:224	arg1	biodegradation					193:206	biodegradation	193:206	biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions	193:338	The present study evaluates biodegradation of the polyblends of poly(lactic acid) (PLA), polycaprolactone (PCL) and microcrystalline cellulose (MCC) in different compositions and comparison of the properties of those blends with that of neat PLA and neat PCL.					
32069712	6	75	theme	structures	1150:1159	arg1	formation					1125:1133	the formation	1121:1133	the formation of fibril-like structures by PLA	1121:1166	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	7	76	from	fuels	1277:1281	arg1	characterization					1308:1323	the material characterization	1295:1323	the material characterization of PLA/PCL/MCC based polyblends	1295:1355	This work fuels interest in the material characterization of PLA/PCL/MCC based polyblends and helps in tuning the biodegradability of the studied samples according to the demands.					
32069712	6	77	from	surface	1215:1221	arg1	formation					1177:1185	the formation	1173:1185	the formation of rough patches on the PCL surface	1173:1221	Field emission scanning electron microscopy showed the formation of fibril-like structures by PLA, and the formation of rough patches on the PCL surface re-confirmed biodegradation of the samples.					
32069712	3	78	theme	carbon	719:724	arg1	percentage					741:750	higher carbon mineralization percentage	712:750	higher carbon mineralization percentage	712:750	It was seen that blends with a higher concentration of PCL and MCC in the PLA matrix showed higher carbon mineralization percentage in comparison to the blends having low PCL and MCC components.					
34541971	0	0	theme	solid	69:73	arg1	waste					75:79	municipal solid waste	59:79	municipal solid waste	59:79	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	4	1	theme	moisture	822:829	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	1	theme	moisture	822:829	arg1	content					831:837	moisture content	822:837	moisture content	822:837	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	2	2	theme	non-destructive	499:513	arg1	NDTs					522:525	NDTs	522:525	NDTs	522:525	To achieve this, a series of destructive (DTs) and non-destructive tests (NDTs) can be conducted on the MSW samples in the BLF.					
34541971	2	2	theme	non-destructive	499:513	arg1	tests					515:519	non-destructive tests	499:519	non-destructive tests (NDTs)	499:526	To achieve this, a series of destructive (DTs) and non-destructive tests (NDTs) can be conducted on the MSW samples in the BLF.					
34541971	1	3	theme	temporal	130:137	arg1	changes					139:145	temporal changes	130:145	temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF)	130:265	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	4	4	theme	elemental	862:870	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	4	theme	elemental	862:870	arg1	content					872:878	elemental content	862:878	elemental content	862:878	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	3	5	theme	cells	682:686	arg1	depths					664:669	different depths	654:669	different depths of the two cells of a fully operational BLF in Mumbai, India	654:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	0	6	theme	municipal	59:67	arg1	waste					75:79	municipal solid waste	59:79	municipal solid waste	59:79	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	1	7	theme	efficient	289:297	arg1	landfilling					299:309	(i) efficient landfilling	285:309	(i) efficient landfilling	285:309	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	8	theme	changes	139:145	arg1	Estimation					116:125	Estimation	116:125	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF)	116:265	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	4	9	theme	lignocellulosic	881:895	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	9	theme	lignocellulosic	881:895	arg1	content					897:903	lignocellulosic content	881:903	lignocellulosic content (i.e. cellulose, hemicellulose and lignin content)	881:954	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	10	theme	physical	751:758	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	10	theme	physical	751:758	arg1	properties					773:782	the physical and chemical properties	747:782	the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential,	747:985	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	7	11	theme	geomechanical	1558:1570	arg1	parameters					1572:1581	the geomechanical parameters	1554:1581	the geomechanical parameters	1554:1581	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	1	12	dep	deciding	384:391	arg1	iii					379:381	iii	379:381	iii	379:381	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	13	theme	bioreactor	241:250	arg1	BLF					262:264	BLF	262:264	BLF	262:264	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	13	theme	bioreactor	241:250	arg1	landfill					252:259	a bioreactor landfill	239:259	a bioreactor landfill (BLF)	239:265	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	0	14	theme	waste	75:79	arg1	decomposition					42:54	decomposition	42:54	decomposition of municipal solid waste	42:79	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	5	15	theme	surface	1133:1139	arg1	waves					1141:1145	surface waves	1133:1145	surface waves	1133:1145	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	4	16	theme	time	1057:1060	arg1	testing					1031:1037	the laboratory testing	1016:1037	the laboratory testing	1016:1037	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	16	theme	time	1057:1060	arg1	function					1045:1052	a function	1043:1052	a function of time	1043:1060	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	5	17	theme	waves	1141:1145	arg1	analysis					1121:1128	multichannel analysis	1108:1128	multichannel analysis of surface waves	1108:1145	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	5	17	theme	waves	1141:1145	arg1	test					1099:1102	cone penetration test	1082:1102	cone penetration test	1082:1102	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	9	18	theme	stabilized	1923:1932	arg1	MSW					1934:1936	stabilized MSW	1923:1936	the 'stabilized MSW'	1918:1937	As, beyond tcr these parameters remain practically unchanged, which corresponds to the 'stabilized MSW', mining of the BLF can be initiated without further delay.					
34541971	4	19	theme	laboratory	1020:1029	arg1	testing					1031:1037	the laboratory testing	1016:1037	the laboratory testing	1016:1037	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	19	theme	laboratory	1020:1029	arg1	function					1045:1052	a function	1043:1052	a function of time	1043:1060	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	20	dep	cellulose	911:919	arg1	i.e.					906:909	i.e.	906:909	i.e.	906:909	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	3	21	from	India	726:730	arg1	cells					682:686	the two cells	674:686	the two cells of a fully operational BLF in Mumbai, India	674:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	1	22	with	decomposition	343:355	arg1	time					369:372	time	369:372	time	369:372	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	7	23	theme	content	1459:1465	arg1	potential					1487:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential	1416:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW	1416:1502	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	0	24	theme	case	2:5	arg1	study					7:11	A case study	0:11	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.	0:114	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	6	25	theme	wave	1286:1289	arg1	velocity					1291:1298	shear wave velocity)	1280:1299	shear wave velocity)	1280:1299	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	6	26	theme	friction	1261:1268	arg1	ratio					1270:1274	friction ratio	1261:1274	friction ratio	1261:1274	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	7	27	theme	content	1434:1440	arg1	potential					1487:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential	1416:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW	1416:1502	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	1	28	theme	municipal	160:168	arg1	MSW					183:185	MSW	183:185	MSW	183:185	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	28	theme	municipal	160:168	arg1	waste					176:180	municipal solid waste	160:180	municipal solid waste (MSW) in its physico-chemico-geomechanical properties	160:234	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	2	29	theme	tests	515:519	arg1	series					467:472	a series	465:472	a series of destructive (DTs) and non-destructive tests (NDTs)	465:526	To achieve this, a series of destructive (DTs) and non-destructive tests (NDTs) can be conducted on the MSW samples in the BLF.					
34541971	2	30	theme	MSW	552:554	arg1	samples					556:562	the MSW samples	548:562	the MSW samples in the BLF	548:573	To achieve this, a series of destructive (DTs) and non-destructive tests (NDTs) can be conducted on the MSW samples in the BLF.					
34541971	7	31	dep	data	1326:1329	arg1	obtained					1331:1338	obtained	1331:1338	obtained from these tests	1331:1355	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	7	31	dep	data	1326:1329	arg1	reported					1362:1369	reported	1362:1369	reported in the literature	1362:1387	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	6	32	theme	MSW	1308:1310	arg1	resistance					1230:1239	cone resistance	1225:1239	cone resistance	1225:1239	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	6	32	theme	MSW	1308:1310	arg1	resistance					1249:1258	sleeve resistance	1242:1258	sleeve resistance	1242:1258	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	6	32	theme	MSW	1308:1310	arg1	ratio					1270:1274	friction ratio	1261:1274	friction ratio	1261:1274	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	6	32	theme	MSW	1308:1310	arg1	velocity					1291:1298	shear wave velocity)	1280:1299	shear wave velocity)	1280:1299	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	1	33	theme	solid	170:174	arg1	MSW					183:185	MSW	183:185	MSW	183:185	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	33	theme	solid	170:174	arg1	waste					176:180	municipal solid waste	160:180	municipal solid waste (MSW) in its physico-chemico-geomechanical properties	160:234	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	8	34	dep	significantly	1689:1701	arg1	%					1707:1707	≈62%	1704:1707	≈62%	1704:1707	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	8	34	dep	significantly	1689:1701	arg1	%					1712:1712	70%	1710:1712	70%	1710:1712	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	8	34	dep	significantly	1689:1701	arg1	%					1717:1717	50%	1715:1717	50%	1715:1717	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	8	34	dep	significantly	1689:1701	arg1	%					1725:1725	30%	1723:1725	30%	1723:1725	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	1	35	dep	landfilling	299:309	arg1	i					286:286	i	286:286	i	286:286	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	8	36	theme	exponential	1756:1766	arg1	decay					1768:1772	an exponential decay	1753:1772	an exponential decay	1753:1772	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	4	37	dep	content	897:903	arg1	hemicellulose					922:934	hemicellulose	922:934	hemicellulose	922:934	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	37	dep	content	897:903	arg1	content					947:953	lignin content	940:953	lignin content	940:953	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	5	38	theme	multichannel	1108:1119	arg1	analysis					1121:1128	multichannel analysis	1108:1128	multichannel analysis of surface waves	1108:1145	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	8	39	theme	critical	1785:1792	arg1	tcr					1800:1802	tcr	1800:1802	tcr	1800:1802	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	8	39	theme	critical	1785:1792	arg1	≈4 years					1806:1813	≈4 years	1806:1813	≈4 years	1806:1813	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	8	39	theme	critical	1785:1792	arg1	time					1794:1797	the critical time	1781:1797	the critical time (tcr) (≈4 years)	1781:1814	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	6	40	theme	cone	1225:1228	arg1	resistance					1230:1239	cone resistance	1225:1239	cone resistance	1225:1239	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	3	41	theme	several	595:601	arg1	DTs					603:605	several DTs	595:605	several DTs	595:605	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	0	42	theme	landfill	97:104	arg1	bioreactor					86:95	a bioreactor	84:95	a bioreactor landfill in India	84:113	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	2	43	from	samples	556:562	arg1	BLF					571:573	the BLF	567:573	the BLF	567:573	To achieve this, a series of destructive (DTs) and non-destructive tests (NDTs) can be conducted on the MSW samples in the BLF.					
34541971	4	44	theme	lignin	940:945	arg1	content					947:953	lignin content	940:953	lignin content	940:953	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	6	45	theme	shear	1280:1284	arg1	velocity					1291:1298	shear wave velocity)	1280:1299	shear wave velocity)	1280:1299	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	7	46	theme	lignocellulosic	1443:1457	arg1	content					1459:1465	lignocellulosic content	1443:1465	lignocellulosic content	1443:1465	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	3	47	from	cells	682:686	arg1	India					726:730	India	726:730	India	726:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	5	48	dep	parameters	1208:1217	arg1	viz					1220:1222	viz	1220:1222	geomechanical parameters (viz	1194:1222	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	7	49	theme	MSW	1500:1502	arg1	potential					1487:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential	1416:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW	1416:1502	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	4	50	theme	chemical	764:771	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	50	theme	chemical	764:771	arg1	properties					773:782	the physical and chemical properties	747:782	the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential,	747:985	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	1	51	theme	physico-chemico-geomechanical	195:223	arg1	properties					225:234	its physico-chemico-geomechanical properties	191:234	its physico-chemico-geomechanical properties	191:234	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	52	theme	appropriate	402:412	arg1	time					414:417	the appropriate time	398:417	the appropriate time to initiate landfill mining	398:445	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	4	53	theme	samples	793:799	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	53	theme	samples	793:799	arg1	properties					773:782	the physical and chemical properties	747:782	the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential,	747:985	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	7	54	theme	VS	1420:1421	arg1	potential					1487:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential	1416:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW	1416:1502	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	7	55	theme	well-defined	1518:1529	arg1	trends					1531:1536	very well-defined trends	1513:1536	very well-defined trends	1513:1536	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	4	56	theme	volatile	840:847	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	56	theme	volatile	840:847	arg1	solids					849:854	volatile solids	840:854	volatile solids	840:854	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	2	57	theme	destructive	477:487	arg1	series					467:472	a series	465:472	a series of destructive (DTs) and non-destructive tests (NDTs)	465:526	To achieve this, a series of destructive (DTs) and non-destructive tests (NDTs) can be conducted on the MSW samples in the BLF.					
34541971	1	58	theme	decomposition	343:355	arg1	state					334:338	the state	330:338	the state of decomposition of MSW with time	330:372	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	1	59	from	waste	176:180	arg1	properties					225:234	its physico-chemico-geomechanical properties	191:234	its physico-chemico-geomechanical properties	191:234	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	3	60	theme	operational	699:709	arg1	BLF					711:713	a fully operational BLF	691:713	a fully operational BLF in Mumbai, India	691:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	8	61	dep	observed	1620:1627	arg1	until					1775:1779	until	1775:1779	until	1775:1779	Furthermore, it has been observed that the VS, hydrogen-, carbon- and nitrogen-content reduce significantly (≈62%, 70%, 50% and 30%, respectively), following an exponential decay, until the critical time (tcr) (≈4 years) has been achieved.					
34541971	5	62	theme	geomechanical	1194:1206	arg1	parameters					1208:1217	geomechanical parameters	1194:1217	geomechanical parameters (viz	1194:1222	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	3	63	theme	BLF	711:713	arg1	cells					682:686	the two cells	674:686	the two cells of a fully operational BLF in Mumbai, India	674:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	3	64	from	BLF	711:713	arg1	India					726:730	India	726:730	India	726:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	7	65	theme	elemental	1424:1432	arg1	content					1434:1440	elemental content	1424:1440	elemental content	1424:1440	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	1	66	theme	MSW	360:362	arg1	decomposition					343:355	decomposition	343:355	decomposition of MSW with time	343:372	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	5	67	theme	BLF	1180:1182	arg1	cells					1171:1175	these cells	1165:1175	these cells of BLF	1165:1182	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	1	68	theme	landfill	431:438	arg1	mining					440:445	landfill mining	431:445	landfill mining	431:445	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	4	69	theme	bio-methanation	960:974	arg1	composition					809:819	composition	809:819	composition	809:819	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	4	69	theme	bio-methanation	960:974	arg1	potential					976:984	bio-methanation potential	960:984	bio-methanation potential	960:984	Subsequently, the physical and chemical properties of these samples such as composition, moisture content, volatile solids (VS), elemental content, lignocellulosic content (i.e. cellulose, hemicellulose and lignin content) and bio-methanation potential, were determined by following the laboratory testing, as a function of time.					
34541971	5	70	theme	penetration	1087:1097	arg1	test					1099:1102	cone penetration test	1082:1102	cone penetration test	1082:1102	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	0	71	from	India	109:113	arg1	landfill					97:104	landfill	97:104	landfill	97:104	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	9	72	theme	BLF	1954:1956	arg1	mining					1940:1945	mining	1940:1945	mining of the BLF	1940:1956	As, beyond tcr these parameters remain practically unchanged, which corresponds to the 'stabilized MSW', mining of the BLF can be initiated without further delay.					
34541971	9	73	theme	further	1983:1989	arg1	delay					1991:1995	further delay	1983:1995	further delay	1983:1995	As, beyond tcr these parameters remain practically unchanged, which corresponds to the 'stabilized MSW', mining of the BLF can be initiated without further delay.					
34541971	5	74	theme	cone	1082:1085	arg1	test					1099:1102	cone penetration test	1082:1102	cone penetration test	1082:1102	Also, NDTs such as cone penetration test and multichannel analysis of surface waves were conducted on these cells of BLF to obtain geomechanical parameters (viz.					
34541971	1	75	dep	establishing	317:328	arg1	ii					313:314	ii	313:314	ii	313:314	Estimation of temporal changes undergone by municipal solid waste (MSW) in its physico-chemico-geomechanical properties in a bioreactor landfill (BLF) is essential for: (i) efficient landfilling, (ii) establishing the state of decomposition of MSW with time and (iii) deciding upon the appropriate time to initiate landfill mining.					
34541971	0	76	theme	decomposition	42:54	arg1	state					33:37	the state	29:37	the state of decomposition of municipal solid waste	29:79	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
34541971	3	77	theme	different	654:662	arg1	depths					664:669	different depths	654:669	different depths of the two cells of a fully operational BLF in Mumbai, India	654:730	With this in view, several DTs were conducted on these samples retrieved from different depths of the two cells of a fully operational BLF in Mumbai, India.					
34541971	6	78	theme	sleeve	1242:1247	arg1	resistance					1249:1258	sleeve resistance	1242:1258	sleeve resistance	1242:1258	cone resistance, sleeve resistance, friction ratio and shear wave velocity) of the MSW.					
34541971	7	79	theme	bio-methanation	1471:1485	arg1	potential					1487:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential	1416:1495	the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW	1416:1502	Based on the data obtained from these tests, and reported in the literature, it has been observed that the VS, elemental content, lignocellulosic content and bio-methanation potential of MSW exhibits very well-defined trends, as compared to the geomechanical parameters, with time.					
34541971	0	80	from	landfill	97:104	arg1	India					109:113	India	109:113	India	109:113	A case study on establishing the state of decomposition of municipal solid waste in a bioreactor landfill in India.					
32659627	7	0	theme	PAX	1142:1144	arg1	attractions					1123:1133	the Van der Waals attractions	1105:1133	the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues	1105:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	7	1	theme	chitosan	1053:1060	arg1	polymer					1068:1074	the trimethyl chitosan (TMC) polymer	1039:1074	the trimethyl chitosan (TMC) polymer	1039:1074	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	2	2	theme	DOX	318:320	arg1	loading					289:295	loading	289:295	loading	289:295	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	7	3	dep	PAX	1142:1144	arg1	the					1138:1140	the	1138:1140	the	1138:1140	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	3	4	theme	Fullerene	506:514	arg1	carrier					516:522	the Fullerene carrier	502:522	the Fullerene carrier	502:522	The effects of chitosan polymer as a functionalizing agent of the Fullerene carrier was also studied.					
32659627	6	5	with	comparison	1011:1020	arg1	PAX					1027:1029	PAX	1027:1029	PAX	1027:1029	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	9	6	theme	significant	1506:1516	arg1	rise					1518:1521	a significant rise	1504:1521	a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH	1504:1597	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	9	7	dep	PAX	1567:1569	arg1	the					1563:1565	the	1563:1565	the	1563:1565	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	2	8	from	DOX	318:320	arg1	carrier					375:381	the pH sensitive functionalized Fullerene carrier	333:381	the pH sensitive functionalized Fullerene carrier	333:381	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	6	9	theme	Waals	909:913	arg1	interactions					915:926	the electrostatic and Van der Waals interactions	879:926	the electrostatic and Van der Waals interactions	879:926	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	2	10	from	PAX	326:328	arg1	carrier					375:381	the pH sensitive functionalized Fullerene carrier	333:381	the pH sensitive functionalized Fullerene carrier	333:381	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	0	11	theme	drugs	133:137	arg1	release					110:116	release	110:116	release	110:116	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	0	11	theme	drugs	133:137	arg1	adsorption					95:104	pH-responsive adsorption	81:104	pH-responsive adsorption	81:104	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	11	12	theme	nanomedicine	1807:1818	arg1	system					1820:1825	this smart nanomedicine system	1796:1825	this smart nanomedicine system	1796:1825	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	11	12	theme	nanomedicine	1807:1818	arg1	composition					1857:1867	an promising composition	1844:1867	an promising composition for smart cancer therapy	1844:1892	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	5	13	theme	carboxyl	781:788	arg1	group					790:794	carboxyl group	781:794	carboxyl group	781:794	The results showed the DOX is well adsorbed on Fullerene which was functionalized with carboxyl group and it was released controllably in cancerous tissues.					
32659627	6	14	theme	Van	901:903	arg1	interactions					915:926	the electrostatic and Van der Waals interactions	879:926	the electrostatic and Van der Waals interactions	879:926	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	9	15	theme	hydrogen	1540:1547	arg1	bonds					1549:1553	hydrogen bonds	1540:1553	hydrogen bonds between the PAX and Fullerene at neutral pH	1540:1597	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	6	16	theme	electrostatic	883:895	arg1	interactions					915:926	the electrostatic and Van der Waals interactions	879:926	the electrostatic and Van der Waals interactions	879:926	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	11	17	theme	smart	1873:1877	arg1	therapy					1886:1892	smart cancer therapy	1873:1892	smart cancer therapy	1873:1892	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	2	18	dep	loading	289:295	arg1	the					285:287	the	285:287	the	285:287	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	7	19	from	release	1279:1285	arg1	tissues					1311:1317	cancerous tissues	1301:1317	cancerous tissues	1301:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	6	20	theme	functionalized	951:964	arg1	carrier					992:998	a proper carrier	983:998	a proper carrier for DOX	983:1006	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	6	20	theme	functionalized	951:964	arg1	Fullerene					966:974	the functionalized Fullerene	947:974	the functionalized Fullerene	947:974	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	7	21	theme	der	1223:1225	arg1	energy					1233:1238	the Van der Waals energy	1215:1238	the Van der Waals energy	1215:1238	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	5	22	theme	cancerous	832:840	arg1	tissues					842:848	cancerous tissues	832:848	cancerous tissues	832:848	The results showed the DOX is well adsorbed on Fullerene which was functionalized with carboxyl group and it was released controllably in cancerous tissues.					
32659627	9	23	theme	neutral	1588:1594	arg1	pH					1596:1597	neutral pH	1588:1597	neutral pH	1588:1597	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	4	24	theme	releasing	653:661	arg1	properties					663:672	releasing properties	653:672	releasing properties	653:672	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	0	25	theme	pH-responsive	81:93	arg1	adsorption					95:104	pH-responsive adsorption	81:104	pH-responsive adsorption	81:104	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	2	26	from	loading	289:295	arg1	carrier					375:381	the pH sensitive functionalized Fullerene carrier	333:381	the pH sensitive functionalized Fullerene carrier	333:381	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	8	27	theme	functionalizing	1363:1377	arg1	agent					1379:1383	a functionalizing agent	1361:1383	a functionalizing agent	1361:1383	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	8	27	theme	functionalizing	1363:1377	arg1	group					1333:1337	The carboxyl group	1320:1337	The carboxyl group which was employed as a functionalizing agent	1320:1383	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	7	28	theme	der	1113:1115	arg1	attractions					1123:1133	the Van der Waals attractions	1105:1133	the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues	1105:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	2	29	theme	smart	264:268	arg1	carriers					275:282	smart drug carriers	264:282	smart drug carriers	264:282	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	4	30	theme	DOX	681:683	arg1	loading					641:647	loading	641:647	loading	641:647	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	4	30	theme	DOX	681:683	arg1	properties					663:672	releasing properties	653:672	releasing properties	653:672	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	2	31	theme	functionalized	350:363	arg1	carrier					375:381	the pH sensitive functionalized Fullerene carrier	333:381	the pH sensitive functionalized Fullerene carrier	333:381	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	7	32	theme	Van	1219:1221	arg1	energy					1233:1238	the Van der Waals energy	1215:1238	the Van der Waals energy	1215:1238	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	4	33	theme	PAX	689:691	arg1	loading					641:647	loading	641:647	loading	641:647	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	4	33	theme	PAX	689:691	arg1	properties					663:672	releasing properties	653:672	releasing properties	653:672	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	0	34	theme	Molecular	0:8	arg1	insight					10:16	Molecular insight	0:16	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.	0:138	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	2	35	theme	molecular	403:411	arg1	dynamics					413:420	molecular dynamics	403:420	molecular dynamics (MD) simulations	403:437	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	2	35	theme	molecular	403:411	arg1	MD					423:424	MD	423:424	MD	423:424	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	7	36	theme	acidic	1204:1209	arg1	pH					1211:1212	acidic pH	1204:1212	acidic pH	1204:1212	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	8	37	theme	hydrogen	1419:1426	arg1	bonds					1428:1432	hydrogen bonds	1419:1432	hydrogen bonds	1419:1432	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	10	38	theme	gyration	1665:1672	arg1	radius					1674:1679	gyration radius	1665:1679	gyration radius	1665:1679	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	7	39	theme	TMC	1063:1065	arg1	polymer					1068:1074	the trimethyl chitosan (TMC) polymer	1039:1074	the trimethyl chitosan (TMC) polymer	1039:1074	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	2	40	from	carrier	375:381	arg1	loading					289:295	loading	289:295	loading	289:295	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	8	41	theme	acidic	1457:1462	arg1	pH					1476:1477	acidic and neutral pH	1457:1477	pH	1476:1477	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	10	42	theme	acidic	1709:1714	arg1	pH					1716:1717	acidic pH	1709:1717	acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier	1709:1789	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	7	43	theme	trimethyl	1043:1051	arg1	polymer					1068:1074	the trimethyl chitosan (TMC) polymer	1039:1074	the trimethyl chitosan (TMC) polymer	1039:1074	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	3	44	theme	polymer	464:470	arg1	effects					444:450	The effects	440:450	The effects of chitosan polymer as a functionalizing agent of the Fullerene carrier	440:522	The effects of chitosan polymer as a functionalizing agent of the Fullerene carrier was also studied.					
32659627	8	45	theme	neutral	1468:1474	arg1	pH					1476:1477	acidic and neutral pH	1457:1477	pH	1476:1477	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	6	46	from	carrier	992:998	arg1	comparison					1011:1020	comparison	1011:1020	comparison with PAX	1011:1029	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	2	47	theme	drug	270:273	arg1	carriers					275:282	smart drug carriers	264:282	smart drug carriers	264:282	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	7	48	theme	cancerous	1301:1309	arg1	tissues					1311:1317	cancerous tissues	1301:1317	cancerous tissues	1301:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	0	49	theme	TMC-Fullerene	48:60	arg1	nanocarrier					62:72	the smart functionalized TMC-Fullerene nanocarrier	23:72	the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs	23:137	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	7	50	theme	PAX	1294:1296	arg1	release					1279:1285	the release	1275:1285	the release of the PAX in cancerous tissues	1275:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	6	51	theme	interactions	915:926	arg1	results					868:874	the results	864:874	the results of the electrostatic and Van der Waals interactions	864:926	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	3	52	theme	carrier	516:522	arg1	agent					493:497	a functionalizing agent	475:497	a functionalizing agent of the Fullerene carrier	475:522	The effects of chitosan polymer as a functionalizing agent of the Fullerene carrier was also studied.					
32659627	6	53	theme	der	905:907	arg1	interactions					915:926	the electrostatic and Van der Waals interactions	879:926	the electrostatic and Van der Waals interactions	879:926	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	5	54	dep	DOX	717:719	arg1	adsorbed					729:736	adsorbed	729:736	is well adsorbed on Fullerene which was functionalized with carboxyl group	721:794	The results showed the DOX is well adsorbed on Fullerene which was functionalized with carboxyl group and it was released controllably in cancerous tissues.					
32659627	8	55	dep	PAX	1442:1444	arg1	the					1438:1440	the	1438:1440	the	1438:1440	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	9	56	theme	bonds	1549:1553	arg1	number					1530:1535	the number	1526:1535	the number of hydrogen bonds between the PAX and Fullerene at neutral pH	1526:1597	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	8	57	theme	carboxyl	1324:1331	arg1	agent					1379:1383	a functionalizing agent	1361:1383	a functionalizing agent	1361:1383	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	8	57	theme	carboxyl	1324:1331	arg1	group					1333:1337	The carboxyl group	1320:1337	The carboxyl group which was employed as a functionalizing agent	1320:1383	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	10	58	with	DOX	1743:1745	arg1	TMC-Fullerene					1752:1764	TMC-Fullerene	1752:1764	TMC-Fullerene	1752:1764	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	4	59	theme	carboxyl	601:608	arg1	groups					610:615	carboxyl groups	601:615	carboxyl groups	601:615	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	10	60	theme	stable	1776:1781	arg1	DOX					1743:1745	the DOX	1739:1745	the DOX with TMC-Fullerene	1739:1764	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	10	60	theme	stable	1776:1781	arg1	carrier					1783:1789	a more stable carrier	1769:1789	a more stable carrier	1769:1789	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	11	61	theme	cancer	1879:1884	arg1	therapy					1886:1892	smart cancer therapy	1873:1892	smart cancer therapy	1873:1892	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	4	62	dep	DOX	681:683	arg1	the					677:679	the	677:679	the	677:679	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	8	63	from	pH	1476:1477	arg1	DOX					1450:1452	DOX	1450:1452	DOX	1450:1452	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	8	63	from	pH	1476:1477	arg1	PAX					1442:1444	PAX	1442:1444	PAX	1442:1444	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	10	64	theme	more	1648:1651	arg1	decrease					1653:1660	A more decrease	1646:1660	A more decrease of gyration radius	1646:1679	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	11	65	theme	smart	1801:1805	arg1	system					1820:1825	this smart nanomedicine system	1796:1825	this smart nanomedicine system	1796:1825	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	11	65	theme	smart	1801:1805	arg1	composition					1857:1867	an promising composition	1844:1867	an promising composition for smart cancer therapy	1844:1892	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	7	66	theme	Waals	1227:1231	arg1	energy					1233:1238	the Van der Waals energy	1215:1238	the Van der Waals energy	1215:1238	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	7	67	theme	Waals	1117:1121	arg1	attractions					1123:1133	the Van der Waals attractions	1105:1133	the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues	1105:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	11	68	theme	promising	1847:1855	arg1	system					1820:1825	this smart nanomedicine system	1796:1825	this smart nanomedicine system	1796:1825	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	11	68	theme	promising	1847:1855	arg1	composition					1857:1867	an promising composition	1844:1867	an promising composition for smart cancer therapy	1844:1892	So, this smart nanomedicine system is introduced as an promising composition for smart cancer therapy.					
32659627	7	69	theme	Van	1109:1111	arg1	attractions					1123:1133	the Van der Waals attractions	1105:1133	the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues	1105:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	0	70	from	nanocarrier	62:72	arg1	release					110:116	release	110:116	release	110:116	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	0	70	from	nanocarrier	62:72	arg1	adsorption					95:104	pH-responsive adsorption	81:104	pH-responsive adsorption	81:104	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	2	71	dep	DOX	318:320	arg1	the					314:316	the	314:316	the	314:316	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	0	72	theme	anti-cancer	121:131	arg1	drugs					133:137	anti-cancer drugs	121:137	anti-cancer drugs	121:137	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	4	73	dep	loading	641:647	arg1	the					637:639	the	637:639	the	637:639	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	2	74	theme	sensitive	340:348	arg1	carrier					375:381	the pH sensitive functionalized Fullerene carrier	333:381	the pH sensitive functionalized Fullerene carrier	333:381	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	9	75	from	rise	1518:1521	arg1	number					1530:1535	the number	1526:1535	the number of hydrogen bonds between the PAX and Fullerene at neutral pH	1526:1597	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	0	76	dep	adsorption	95:104	arg1	the					77:79	the	77:79	the	77:79	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	2	77	theme	dynamics	413:420	arg1	simulations					427:437	molecular dynamics (MD) simulations	403:437	molecular dynamics (MD) simulations	403:437	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	6	78	theme	proper	985:990	arg1	carrier					992:998	a proper carrier	983:998	a proper carrier for DOX	983:1006	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	6	78	theme	proper	985:990	arg1	Fullerene					966:974	the functionalized Fullerene	947:974	the functionalized Fullerene	947:974	According to the results of the electrostatic and Van der Waals interactions, it was found that the functionalized Fullerene can be a proper carrier for DOX in comparison with PAX.					
32659627	8	79	theme	bonds	1428:1432	arg1	number					1409:1414	the number	1405:1414	the number of hydrogen bonds	1405:1432	The carboxyl group which was employed as a functionalizing agent could also increase the number of hydrogen bonds for the PAX and DOX at acidic and neutral pH, respectively.					
32659627	10	80	theme	radius	1674:1679	arg1	decrease					1653:1660	A more decrease	1646:1660	A more decrease of gyration radius	1646:1679	A more decrease of gyration radius was obtained for the DOX at acidic pH which confirms that the DOX with TMC-Fullerene is a more stable carrier.					
32659627	0	81	theme	functionalized	33:46	arg1	nanocarrier					62:72	the smart functionalized TMC-Fullerene nanocarrier	23:72	the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs	23:137	Molecular insight into the smart functionalized TMC-Fullerene nanocarrier in the pH-responsive adsorption and release of anti-cancer drugs.					
32659627	2	82	theme	Fullerene	365:373	arg1	carrier					375:381	the pH sensitive functionalized Fullerene carrier	333:381	the pH sensitive functionalized Fullerene carrier	333:381	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	3	83	theme	chitosan	455:462	arg1	polymer					464:470	chitosan polymer	455:470	chitosan polymer	455:470	The effects of chitosan polymer as a functionalizing agent of the Fullerene carrier was also studied.					
32659627	9	84	from	pH	1596:1597	arg1	Fullerene					1575:1583	Fullerene	1575:1583	Fullerene	1575:1583	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	9	84	from	pH	1596:1597	arg1	PAX					1567:1569	PAX	1567:1569	PAX	1567:1569	Moreover, a significant rise in the number of hydrogen bonds between the PAX and Fullerene at neutral pH was achieved by adding the TMC to the carrier.					
32659627	1	85	used	used	194:197	arg2	Paclitaxel					166:175	Paclitaxel	166:175	Paclitaxel (PAX)	166:181	The Doxorubicin (DOX) and Paclitaxel (PAX) are widely used for cancer-therapy.					
32659627	1	85	used	used	194:197	arg2	DOX					157:159	DOX	157:159	DOX	157:159	The Doxorubicin (DOX) and Paclitaxel (PAX) are widely used for cancer-therapy.					
32659627	1	85	used	used	194:197	arg2	Doxorubicin					144:154	The Doxorubicin	140:154	The Doxorubicin (DOX)	140:160	The Doxorubicin (DOX) and Paclitaxel (PAX) are widely used for cancer-therapy.					
32659627	1	85	used	used	194:197	arg2	PAX					178:180	PAX	178:180	PAX	178:180	The Doxorubicin (DOX) and Paclitaxel (PAX) are widely used for cancer-therapy.					
32659627	4	86	theme	Fullerene	559:567	arg1	carrier					569:575	the Fullerene carrier	555:575	the Fullerene carrier	555:575	In addition, the Fullerene carrier was functionalized with carboxyl groups in order to improve the loading and releasing properties of the DOX and PAX.					
32659627	2	87	theme	PAX	326:328	arg1	loading					289:295	loading	289:295	loading	289:295	Herein, in the efforts devoted to developing smart drug carriers, the loading and releasing of the DOX and PAX on the pH sensitive functionalized Fullerene carrier was investigated by molecular dynamics (MD) simulations.					
32659627	7	88	theme	Fullerene	1150:1158	arg1	attractions					1123:1133	the Van der Waals attractions	1105:1133	the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues	1105:1317	Adding the trimethyl chitosan (TMC) polymer to the carrier could improve the Van der Waals attractions of the PAX and Fullerene which indicates that by passing the time at acidic pH, the Van der Waals energy reaches zero that leads to promote the release of the PAX in cancerous tissues.					
32659627	3	89	theme	functionalizing	477:491	arg1	agent					493:497	a functionalizing agent	475:497	a functionalizing agent of the Fullerene carrier	475:522	The effects of chitosan polymer as a functionalizing agent of the Fullerene carrier was also studied.					
32116753	0	0	theme	Ground	89:94	arg1	Squirrels					96:104	Hibernating Daurian Ground Squirrels	69:104	Hibernating Daurian Ground Squirrels (Spermophilus dauricus)	69:128	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	2	1	theme	muscle	493:498	arg1	atrophy					500:506	disuse muscle atrophy	486:506	disuse muscle atrophy prevention	486:517	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	4	2	theme	protein	1093:1099	arg1	sialylation					1101:1111	protein sialylation	1093:1111	protein sialylation homeostasis	1093:1123	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	1	3	theme	post-translational	150:167	arg1	glycosylation					191:203	glycosylation	191:203	glycosylation	191:203	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	1	3	theme	post-translational	150:167	arg1	modification					177:188	the most common post-translational protein modification	134:188	the most common post-translational protein modification	134:188	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	4	4	theme	Daurian	1059:1065	arg1	squirrels					1074:1082	hibernating Daurian ground squirrels	1047:1082	hibernating Daurian ground squirrels	1047:1082	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	0	5	theme	Daurian	81:87	arg1	Squirrels					96:104	Hibernating Daurian Ground Squirrels	69:104	Hibernating Daurian Ground Squirrels (Spermophilus dauricus)	69:128	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	0	6	from	Homeostasis	11:21	arg1	Muscles					58:64	Skeletal Muscles	49:64	Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus)	49:128	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	1	7	theme	protein	169:175	arg1	glycosylation					191:203	glycosylation	191:203	glycosylation	191:203	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	1	7	theme	protein	169:175	arg1	modification					177:188	the most common post-translational protein modification	134:188	the most common post-translational protein modification	134:188	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	2	8	from	muscle	332:337	arg1	squirrels					363:371	Daurian ground squirrels	348:371	Daurian ground squirrels (Spermophilus dauricus)	348:395	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	9	theme	sialic	583:588	arg1	SAα2-3Gal					611:619	SAα2-3Gal	611:619	SAα2-3Gal	611:619	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	9	theme	sialic	583:588	arg1	galactose					600:608	sialic acid α2-3 galactose	583:608	sialic acid α2-3 galactose (SAα2-3Gal)	583:620	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	10	theme	glycosylation	304:316	arg1	performance					281:291	the performance	277:291	the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus)	277:395	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	11	theme	shock	871:875	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	11	theme	shock	871:875	arg1	expression					912:921	expression	912:921	expression of 14-3-3 epsilon protein	912:947	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	0	12	theme	Squirrels	96:104	arg1	Muscles					58:64	Skeletal Muscles	49:64	Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus)	49:128	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	2	13	theme	protein	296:302	arg1	glycosylation					304:316	protein glycosylation	296:316	protein glycosylation	296:316	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	14	dep	modifications	849:861	arg1	3					823:823	3	823:823	3	823:823	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	0	15	theme	Remarkable	0:9	arg1	Homeostasis					11:21	Remarkable Homeostasis	0:21	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).	0:129	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	3	16	theme	kinase	901:906	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	16	theme	kinase	901:906	arg1	expression					912:921	expression	912:921	expression of 14-3-3 epsilon protein	912:947	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	4	17	theme	skeletal	1027:1034	arg1	muscles					1036:1042	the skeletal muscles	1023:1042	the skeletal muscles of hibernating Daurian ground squirrels	1023:1082	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	4	18	theme	interbout	1179:1187	arg1	arousal					1189:1195	periodic interbout arousal	1170:1195	periodic interbout arousal	1170:1195	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	4	19	theme	periodic	1170:1177	arg1	arousal					1189:1195	periodic interbout arousal	1170:1195	periodic interbout arousal	1170:1195	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	3	20	link	α2-3-linked	826:836	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	21	from	changes	731:737	arg1	levels					757:762	the expression levels	742:762	the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases	742:815	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	0	22	dep	Squirrels	96:104	arg1	dauricus					120:127	Spermophilus dauricus	107:127	Spermophilus dauricus	107:127	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	2	23	theme	glycosylation	444:456	arg1	role					428:431	the potential role	414:431	the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention	414:517	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	2	24	theme	disuse	486:491	arg1	atrophy					500:506	disuse muscle atrophy	486:506	disuse muscle atrophy prevention	486:517	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	25	theme	pyruvate	892:899	arg1	kinase					901:906	pyruvate kinase	892:906	pyruvate kinase	892:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	26	theme	protein	436:442	arg1	glycosylation					444:456	protein glycosylation	436:456	protein glycosylation	436:456	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	27	theme	glycan	554:559	arg1	structures					561:570	seven glycan structures	548:570	seven glycan structures	548:570	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	28	gly	sialylated	838:847	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	29	theme	soleus	325:330	arg1	SOL					340:342	SOL	340:342	SOL	340:342	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	2	29	theme	soleus	325:330	arg1	muscle					332:337	the soleus muscle	321:337	the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus)	321:395	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	30	theme	14-3-3	926:931	arg1	protein					941:947	14-3-3 epsilon protein	926:947	14-3-3 epsilon protein	926:947	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	0	31	theme	Protein	26:32	arg1	Sialylation					34:44	Protein Sialylation	26:44	Protein Sialylation	26:44	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	3	32	theme	epsilon	933:939	arg1	protein					941:947	14-3-3 epsilon protein	926:947	14-3-3 epsilon protein	926:947	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	33	theme	potential	418:426	arg1	role					428:431	the potential role	414:431	the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention	414:517	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	4	34	theme	disuse	1247:1252	arg1	atrophy					1254:1260	disuse atrophy	1247:1260	disuse atrophy	1247:1260	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	2	35	theme	Daurian	348:354	arg1	squirrels					363:371	Daurian ground squirrels	348:371	Daurian ground squirrels (Spermophilus dauricus)	348:395	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	36	dep	comprising	572:581	arg1	1					545:545	1	545:545	1	545:545	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	4	37	theme	sialylation	1138:1148	arg1	modification					1150:1161	sialylation modification	1138:1161	sialylation modification	1138:1161	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	3	38	from	alterations	659:669	arg1	structure					688:696	the SAα2-3Gal structure	674:696	the SAα2-3Gal structure during hibernation	674:715	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	39	theme	heat	866:869	arg1	shock					871:875	heat shock cognate 70	866:886	heat shock cognate 70	866:886	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	40	theme	α2-3	595:598	arg1	SAα2-3Gal					611:619	SAα2-3Gal	611:619	SAα2-3Gal	611:619	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	40	theme	α2-3	595:598	arg1	galactose					600:608	sialic acid α2-3 galactose	583:608	sialic acid α2-3 galactose (SAα2-3Gal)	583:620	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	0	41	theme	Sialylation	34:44	arg1	Homeostasis					11:21	Remarkable Homeostasis	0:21	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).	0:129	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	4	42	theme	hibernating	1047:1057	arg1	squirrels					1074:1082	hibernating Daurian ground squirrels	1047:1082	hibernating Daurian ground squirrels	1047:1082	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	1	43	theme	muscle	229:234	arg1	atrophy					236:242	muscle atrophy	229:242	muscle atrophy	229:242	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	3	44	theme	beta-galactoside	767:782	arg1	alpha-2					784:790	beta-galactoside alpha-2	767:790	beta-galactoside alpha-2	767:790	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	45	from	role	428:431	arg1	mechanism					465:473	the mechanism	461:473	the mechanism underlying disuse muscle atrophy prevention	461:517	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	4	46	theme	skeletal	1222:1229	arg1	muscles					1231:1237	the skeletal muscles	1218:1237	the skeletal muscles	1218:1237	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	0	47	theme	Skeletal	49:56	arg1	Muscles					58:64	Skeletal Muscles	49:64	Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus)	49:128	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	3	48	theme	expression	746:755	arg1	levels					757:762	the expression levels	742:762	the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases	742:815	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	1	49	attach	linked	219:224	arg2	modification					177:188	the most common post-translational protein modification	134:188	the most common post-translational protein modification	134:188	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	1	49	attach	linked	219:224	arg1	atrophy					236:242	muscle atrophy	229:242	muscle atrophy	229:242	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	1	49	attach	linked	219:224	arg2	glycosylation					191:203	glycosylation	191:203	glycosylation	191:203	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	3	50	theme	acid	590:593	arg1	SAα2-3Gal					611:619	SAα2-3Gal	611:619	SAα2-3Gal	611:619	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	50	theme	acid	590:593	arg1	galactose					600:608	sialic acid α2-3 galactose	583:608	sialic acid α2-3 galactose (SAα2-3Gal)	583:620	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	51	theme	protein	941:947	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	51	theme	protein	941:947	arg1	expression					912:921	expression	912:921	expression of 14-3-3 epsilon protein	912:947	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	4	52	theme	sialylation	1101:1111	arg1	homeostasis					1113:1123	protein sialylation homeostasis	1093:1123	protein sialylation homeostasis	1093:1123	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	2	53	from	performance	281:291	arg1	SOL					340:342	SOL	340:342	SOL	340:342	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	2	53	from	performance	281:291	arg1	muscle					332:337	the soleus muscle	321:337	the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus)	321:395	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	54	theme	3-sialyltransferases	796:815	arg1	levels					757:762	the expression levels	742:762	the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases	742:815	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	55	dep	squirrels	363:371	arg1	dauricus					387:394	Spermophilus dauricus	374:394	Spermophilus dauricus	374:394	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	56	theme	α2-3-linked	826:836	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	0	57	theme	Hibernating	69:79	arg1	Squirrels					96:104	Hibernating Daurian Ground Squirrels	69:104	Hibernating Daurian Ground Squirrels (Spermophilus dauricus)	69:128	Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus).					
32116753	4	58	theme	squirrels	1074:1082	arg1	muscles					1036:1042	the skeletal muscles	1023:1042	the skeletal muscles of hibernating Daurian ground squirrels	1023:1082	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	4	59	theme	ground	1067:1072	arg1	squirrels					1074:1082	hibernating Daurian ground squirrels	1047:1082	hibernating Daurian ground squirrels	1047:1082	Our findings indicate that the skeletal muscles of hibernating Daurian ground squirrels maintain protein sialylation homeostasis by restoring sialylation modification during periodic interbout arousal, which might protect the skeletal muscles against disuse atrophy.					
32116753	2	60	theme	ground	356:361	arg1	squirrels					363:371	Daurian ground squirrels	348:371	Daurian ground squirrels (Spermophilus dauricus)	348:395	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	61	theme	sialylated	838:847	arg1	modifications					849:861	α2-3-linked sialylated modifications	826:861	(3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase	822:906	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	3	62	theme	alpha-2	784:790	arg1	levels					757:762	the expression levels	742:762	the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases	742:815	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	2	63	theme	atrophy	500:506	arg1	prevention					508:517	disuse muscle atrophy prevention	486:517	disuse muscle atrophy prevention	486:517	This study aimed to investigate the performance of protein glycosylation in the soleus muscle (SOL) in Daurian ground squirrels (Spermophilus dauricus) and to determine the potential role of protein glycosylation in the mechanism underlying disuse muscle atrophy prevention.					
32116753	3	64	theme	SAα2-3Gal	678:686	arg1	structure					688:696	the SAα2-3Gal structure	674:696	the SAα2-3Gal structure during hibernation	674:715	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
32116753	1	65	theme	common	143:148	arg1	glycosylation					191:203	glycosylation	191:203	glycosylation	191:203	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	1	65	theme	common	143:148	arg1	modification					177:188	the most common post-translational protein modification	134:188	the most common post-translational protein modification	134:188	As the most common post-translational protein modification, glycosylation is intimately linked to muscle atrophy.					
32116753	3	66	theme	cognate	877:883	arg1	shock					871:875	heat shock cognate 70	866:886	heat shock cognate 70	866:886	The results showed that (1) seven glycan structures comprising sialic acid α2-3 galactose (SAα2-3Gal) were altered during hibernation; (2) alterations in the SAα2-3Gal structure during hibernation were based on changes in the expression levels of beta-galactoside alpha-2 and 3-sialyltransferases; and (3) α2-3-linked sialylated modifications of heat shock cognate 70 and pyruvate kinase and expression of 14-3-3 epsilon protein were oscillatorily changed during hibernation.					
34274715	7	0	theme	better	1153:1158	arg1	fitting					1160:1166	better fitting	1153:1166	better fitting (R2 greater than 0.99) to the pseudo-second-order rate equation	1153:1230	The adsorption data showed better fitting (R2 greater than 0.99) to the pseudo-second-order rate equation.					
34274715	7	0	theme	better	1153:1158	arg1	R2 greater					1169:1178	R2 greater	1169:1178	R2 greater	1169:1178	The adsorption data showed better fitting (R2 greater than 0.99) to the pseudo-second-order rate equation.					
34274715	1	1	theme	porous	304:309	arg1	structure					311:319	porous structure	304:319	porous structure	304:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	10	2	from	fuels	1530:1534	arg1	desulfurization					1509:1523	desulfurization	1509:1523	desulfurization from fuels	1509:1534	All of these features make the PET-AC/Cu(II)-A as a potential adsorbent towards desulfurization from fuels.					
34274715	2	3	dep	Fourier	465:471	arg1	transform					473:481	transform	473:481	transform infrared spectroscopy	473:503	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	5	4	theme	PET-AC/Cu	893:901	arg1	-A					906:907	PET-AC/Cu(II)-A	893:907	PET-AC/Cu(II)-A	893:907	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	0	5	from	performance	9:19	arg1	fuel					74:77	model fuel	68:77	model fuel	68:77	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	5	6	theme	adsorption	973:982	arg1	centers					984:990	adsorption centers	973:990	adsorption centers	973:990	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	4	7	theme	prepared	776:783	arg1	beads					794:798	the prepared Cu(II)-A beads	772:798	the prepared Cu(II)-A beads	772:798	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	5	8	theme	mass	916:919	arg1	ratio					921:925	4:1 mass ratio	912:925	4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers	912:990	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	5	9	theme	4:1	912:914	arg1	ratio					921:925	4:1 mass ratio	912:925	4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers	912:990	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	2	10	theme	Brunauer-Emmett-Teller	506:527	arg1	adsorption					529:538	Brunauer-Emmett-Teller adsorption	506:538	Brunauer-Emmett-Teller adsorption	506:538	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	1	11	with	carbon	283:288	arg1	structure					311:319	porous structure	304:319	porous structure	304:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	8	12	theme	driving	1283:1289	arg1	force					1291:1295	the driving force	1279:1295	the driving force of the DBT adsorption	1279:1317	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	12	theme	driving	1283:1289	arg1	interactions					1257:1268	Lewis acid-base and π-π interactions	1233:1268	interactions	1257:1268	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	12	theme	driving	1283:1289	arg1	acid-base					1239:1247	Lewis acid-base and π-π interactions	1233:1268	acid-base	1239:1247	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	3	13	from	activity	627:634	arg1	oil					693:695	the model oil	683:695	the model oil	683:695	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	7	14	theme	rate	1218:1221	arg1	equation					1223:1230	the pseudo-second-order rate equation	1194:1230	the pseudo-second-order rate equation	1194:1230	The adsorption data showed better fitting (R2 greater than 0.99) to the pseudo-second-order rate equation.					
34274715	0	15	theme	-alginate	94:102	arg1	beads					104:108	-alginate beads	94:108	-alginate beads containing polyethyleneterephthalate	94:145	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	8	16	theme	Lewis	1233:1237	arg1	acid-base					1239:1247	Lewis acid-base and π-π interactions	1233:1268	acid-base	1239:1247	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	16	theme	Lewis	1233:1237	arg1	interactions					1257:1268	Lewis acid-base and π-π interactions	1233:1268	interactions	1257:1268	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	16	theme	Lewis	1233:1237	arg1	force					1291:1295	the driving force	1279:1295	the driving force of the DBT adsorption	1279:1317	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	9	17	theme	successive	1361:1370	arg1	runs					1372:1375	4 successive runs	1359:1375	4 successive runs with negligible loss in desulfurization capability	1359:1426	The adsorbent could be also reused for 4 successive runs with negligible loss in desulfurization capability.					
34274715	9	18	theme	negligible	1382:1391	arg1	loss					1393:1396	negligible loss	1382:1396	negligible loss in desulfurization capability	1382:1426	The adsorbent could be also reused for 4 successive runs with negligible loss in desulfurization capability.					
34274715	5	19	theme	experimental	826:837	arg1	results					839:845	experimental results	826:845	experimental results	826:845	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	7	20	theme	adsorption	1130:1139	arg1	data					1141:1144	The adsorption data	1126:1144	The adsorption data	1126:1144	The adsorption data showed better fitting (R2 greater than 0.99) to the pseudo-second-order rate equation.					
34274715	1	21	contain	containing	228:237	arg2	polyethyleneterephthalate					239:263	polyethyleneterephthalate	239:263	polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure	239:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	1	21	contain	containing	228:237	arg1	beads					222:226	copper(II)-alginate (Cu(II)-A) beads	191:226	copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure	191:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	5	22	theme	high	938:941	arg1	porosity					943:950	its high porosity	934:950	its high porosity	934:950	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	1	23	theme	feasible	340:347	arg1	technology					363:372	a feasible cross-linking technology	338:372	a feasible cross-linking technology	338:372	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	8	24	theme	adsorption	1308:1317	arg1	force					1291:1295	the driving force	1279:1295	the driving force of the DBT adsorption	1279:1317	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	24	theme	adsorption	1308:1317	arg1	interactions					1257:1268	Lewis acid-base and π-π interactions	1233:1268	interactions	1257:1268	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	24	theme	adsorption	1308:1317	arg1	acid-base					1239:1247	Lewis acid-base and π-π interactions	1233:1268	acid-base	1239:1247	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	0	25	theme	activated	155:163	arg1	carbon					165:170	activated carbon	155:170	activated carbon	155:170	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	6	26	theme	adsorption	997:1006	arg1	isotherms					1008:1016	The adsorption isotherms	993:1016	The adsorption isotherms	993:1016	The adsorption isotherms could be correlated by the Langmuir isotherm and the maximum adsorption capacity reached up to 62.9 mg g-1.					
34274715	0	27	theme	Enhanced	0:7	arg1	performance					9:19	Enhanced performance	0:19	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper	0:89	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	4	28	theme	ratio	737:741	arg1	influence					719:727	The influence	715:727	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads	715:798	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	1	29	theme	copper	191:196	arg1	Cu					212:213	Cu(II)-A	212:219	Cu(II)-A	212:219	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	1	29	theme	copper	191:196	arg1	-alginate					201:209	copper(II)-alginate	191:209	copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure	191:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	0	30	theme	adsorptive	24:33	arg1	removal					35:41	adsorptive removal	24:41	adsorptive removal of dibenzothiophene from model fuel	24:77	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	8	31	theme	π-π	1253:1255	arg1	force					1291:1295	the driving force	1279:1295	the driving force of the DBT adsorption	1279:1317	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	31	theme	π-π	1253:1255	arg1	interactions					1257:1268	Lewis acid-base and π-π interactions	1233:1268	interactions	1257:1268	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	8	31	theme	π-π	1253:1255	arg1	acid-base					1239:1247	Lewis acid-base and π-π interactions	1233:1268	acid-base	1239:1247	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	6	32	theme	adsorption	1079:1088	arg1	capacity					1090:1097	the maximum adsorption capacity	1067:1097	the maximum adsorption capacity	1067:1097	The adsorption isotherms could be correlated by the Langmuir isotherm and the maximum adsorption capacity reached up to 62.9 mg g-1.					
34274715	5	33	theme	available	956:964	arg1	Cu					966:967	available Cu	956:967	available Cu(II)	956:971	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	5	33	theme	available	956:964	arg1	II					969:970	II	969:970	II	969:970	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	5	34	dep	porosity	943:950	arg1	centers					984:990	adsorption centers	973:990	adsorption centers	973:990	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	8	35	theme	DBT	1304:1306	arg1	adsorption					1308:1317	the DBT adsorption	1300:1317	the DBT adsorption	1300:1317	Lewis acid-base and π-π interactions might be the driving force of the DBT adsorption.					
34274715	2	36	theme	electron	550:557	arg1	microscopy					559:568	scanning electron microscopy	541:568	scanning electron microscopy	541:568	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	1	37	theme	cross-linking	349:361	arg1	technology					363:372	a feasible cross-linking technology	338:372	a feasible cross-linking technology	338:372	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	4	38	theme	PET-AC	746:751	arg1	ratio					737:741	mass ratio	732:741	mass ratio of PET-AC	732:751	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	5	39	theme	adsorption	855:864	arg1	capacity					866:873	higher adsorption capacity	848:873	higher adsorption capacity	848:873	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	1	40	theme	activated	273:281	arg1	PET-AC					291:296	PET-AC	291:296	PET-AC	291:296	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	1	40	theme	activated	273:281	arg1	carbon					283:288	activated carbon	273:288	activated carbon (PET-AC) with porous structure	273:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	2	41	theme	scanning	541:548	arg1	microscopy					559:568	scanning electron microscopy	541:568	scanning electron microscopy	541:568	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	4	42	theme	-A	791:792	arg1	beads					794:798	the prepared Cu(II)-A beads	772:798	the prepared Cu(II)-A beads	772:798	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	0	43	contain	containing	110:119	arg1	beads					104:108	-alginate beads	94:108	-alginate beads containing polyethyleneterephthalate	94:145	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	0	43	contain	containing	110:119	arg2	polyethyleneterephthalate					121:145	polyethyleneterephthalate	121:145	polyethyleneterephthalate	121:145	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	0	44	theme	removal	35:41	arg1	performance					9:19	Enhanced performance	0:19	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper	0:89	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	2	45	theme	X-ray	446:450	arg1	diffraction					452:462	X-ray diffraction	446:462	X-ray diffraction	446:462	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	0	46	from	fuel	74:77	arg1	performance					9:19	Enhanced performance	0:19	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper	0:89	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	0	46	from	fuel	74:77	arg1	removal					35:41	adsorptive removal	24:41	adsorptive removal of dibenzothiophene from model fuel	24:77	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	2	47	theme	beads	412:416	arg1	structure					395:403	structure	395:403	structure	395:403	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	2	47	theme	beads	412:416	arg1	composition					379:389	composition	379:389	composition	379:389	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	1	48	theme	-alginate	201:209	arg1	beads					222:226	copper(II)-alginate (Cu(II)-A) beads	191:226	copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure	191:319	In this research, copper(II)-alginate (Cu(II)-A) beads containing polyethyleneterephthalate derived activated carbon (PET-AC) with porous structure were prepared by a feasible cross-linking technology.					
34274715	2	49	theme	X-ray	592:596	arg1	methods					598:604	energy dispersive X-ray methods	574:604	energy dispersive X-ray methods	574:604	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	3	50	theme	dibenzothiophene	657:672	arg1	activity					627:634	The desulfurization activity	607:634	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil	607:695	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	9	51	with	runs	1372:1375	arg1	loss					1393:1396	negligible loss	1382:1396	negligible loss in desulfurization capability	1382:1426	The adsorbent could be also reused for 4 successive runs with negligible loss in desulfurization capability.					
34274715	10	52	theme	PET-AC/Cu	1460:1468	arg1	-A					1473:1474	the PET-AC/Cu(II)-A	1456:1474	the PET-AC/Cu(II)-A	1456:1474	All of these features make the PET-AC/Cu(II)-A as a potential adsorbent towards desulfurization from fuels.					
34274715	10	52	theme	PET-AC/Cu	1460:1468	arg1	potential					1481:1489	a potential	1479:1489	a potential adsorbent towards desulfurization from fuels	1479:1534	All of these features make the PET-AC/Cu(II)-A as a potential adsorbent towards desulfurization from fuels.					
34274715	0	53	theme	dibenzothiophene	46:61	arg1	removal					35:41	adsorptive removal	24:41	adsorptive removal of dibenzothiophene from model fuel	24:77	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	2	54	theme	dispersive	581:590	arg1	methods					598:604	energy dispersive X-ray methods	574:604	energy dispersive X-ray methods	574:604	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	4	55	theme	beads	794:798	arg1	features					760:767	the features	756:767	the features of the prepared Cu(II)-A beads	756:798	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	9	56	theme	desulfurization	1401:1415	arg1	capability					1417:1426	desulfurization capability	1401:1426	desulfurization capability	1401:1426	The adsorbent could be also reused for 4 successive runs with negligible loss in desulfurization capability.					
34274715	3	57	theme	for	653:655	arg1	DBT					675:677	DBT	675:677	DBT	675:677	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	3	57	theme	for	653:655	arg1	dibenzothiophene					657:672	the adsorbent for dibenzothiophene	639:672	the adsorbent for dibenzothiophene (DBT)	639:678	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	2	58	theme	energy	574:579	arg1	methods					598:604	energy dispersive X-ray methods	574:604	energy dispersive X-ray methods	574:604	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	3	59	theme	model	687:691	arg1	oil					693:695	the model oil	683:695	the model oil	683:695	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	3	60	theme	desulfurization	611:625	arg1	activity					627:634	The desulfurization activity	607:634	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil	607:695	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	2	61	dep	composition	379:389	arg1	The					375:377	The	375:377	The	375:377	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	4	62	theme	mass	732:735	arg1	ratio					737:741	mass ratio	732:741	mass ratio of PET-AC	732:751	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	5	63	theme	higher	848:853	arg1	capacity					866:873	higher adsorption capacity	848:873	higher adsorption capacity	848:873	According to experimental results, higher adsorption capacity was acquired from PET-AC/Cu(II)-A at 4:1 mass ratio due to its high porosity and available Cu(II) adsorption centers.					
34274715	2	64	dep	transform	473:481	arg1	infrared					483:490	infrared	483:490	transform infrared spectroscopy	473:503	The composition and structure of the beads were thoroughly analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller adsorption, scanning electron microscopy and energy dispersive X-ray methods.					
34274715	3	65	theme	adsorbent	643:651	arg1	DBT					675:677	DBT	675:677	DBT	675:677	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	3	65	theme	adsorbent	643:651	arg1	dibenzothiophene					657:672	the adsorbent for dibenzothiophene	639:672	the adsorbent for dibenzothiophene (DBT)	639:678	The desulfurization activity of the adsorbent for dibenzothiophene (DBT) in the model oil was investigated.					
34274715	6	66	theme	Langmuir	1045:1052	arg1	isotherm					1054:1061	the Langmuir isotherm	1041:1061	the Langmuir isotherm	1041:1061	The adsorption isotherms could be correlated by the Langmuir isotherm and the maximum adsorption capacity reached up to 62.9 mg g-1.					
34274715	4	67	from	influence	719:727	arg1	features					760:767	the features	756:767	the features of the prepared Cu(II)-A beads	756:798	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	4	68	theme	Cu	785:786	arg1	beads					794:798	the prepared Cu(II)-A beads	772:798	the prepared Cu(II)-A beads	772:798	The influence of mass ratio of PET-AC on the features of the prepared Cu(II)-A beads was studied.					
34274715	6	69	dep	62.9 mg g-1	1113:1123	arg1	up					1107:1108	up	1107:1108	up	1107:1108	The adsorption isotherms could be correlated by the Langmuir isotherm and the maximum adsorption capacity reached up to 62.9 mg g-1.					
34274715	9	70	from	loss	1393:1396	arg1	capability					1417:1426	desulfurization capability	1401:1426	desulfurization capability	1401:1426	The adsorbent could be also reused for 4 successive runs with negligible loss in desulfurization capability.					
34274715	0	71	dep	derived	147:153	arg1	II					91:92	II	91:92	II	91:92	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	0	72	theme	model	68:72	arg1	fuel					74:77	model fuel	68:77	model fuel	68:77	Enhanced performance of adsorptive removal of dibenzothiophene from model fuel over copper(II)-alginate beads containing polyethyleneterephthalate derived activated carbon.					
34274715	6	73	theme	maximum	1071:1077	arg1	capacity					1090:1097	the maximum adsorption capacity	1067:1097	the maximum adsorption capacity	1067:1097	The adsorption isotherms could be correlated by the Langmuir isotherm and the maximum adsorption capacity reached up to 62.9 mg g-1.					
34274715	7	74	theme	pseudo-second-order	1198:1216	arg1	equation					1223:1230	the pseudo-second-order rate equation	1194:1230	the pseudo-second-order rate equation	1194:1230	The adsorption data showed better fitting (R2 greater than 0.99) to the pseudo-second-order rate equation.					
34274715	10	75	theme	adsorbent	1491:1499	arg1	-A					1473:1474	the PET-AC/Cu(II)-A	1456:1474	the PET-AC/Cu(II)-A	1456:1474	All of these features make the PET-AC/Cu(II)-A as a potential adsorbent towards desulfurization from fuels.					
34274715	10	75	theme	adsorbent	1491:1499	arg1	potential					1481:1489	a potential	1479:1489	a potential adsorbent towards desulfurization from fuels	1479:1534	All of these features make the PET-AC/Cu(II)-A as a potential adsorbent towards desulfurization from fuels.					
34597635	9	0	theme	pollutants	1978:1987	arg1	adsorption					1960:1969	the simultaneous adsorption	1943:1969	the simultaneous adsorption of the pollutants	1943:1987	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	5	1	theme	diffusion	1372:1380	arg1	model					1382:1386	the intra-particle diffusion model	1353:1386	the intra-particle diffusion model	1353:1386	The adsorption process apparently followed pseudo-second-order (PSO) kinetics for both pollutants, however the adsorption kinetics was also explained by the intra-particle diffusion model.					
34597635	10	2	theme	adsorption	2030:2039	arg1	capacity					2041:2048	its adsorption capacity	2026:2048	its adsorption capacity for both inorganic and organic pollutants	2026:2090	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	10	3	theme	practical	2186:2194	arg1	treatment					2207:2215	practical wastewater treatment	2186:2215	practical wastewater treatment containing both inorganic and organic toxic pollutants	2186:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	6	4	theme	Langmuir	1458:1465	arg1	model					1476:1480	the Langmuir isotherm model	1454:1480	the Langmuir isotherm model	1454:1480	The isotherm data for both metals ions and dyes were well fit by the Langmuir isotherm model.					
34597635	4	5	theme	pH	1062:1063	arg1	time					1081:1084	contact time	1073:1084	contact time	1073:1084	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	5	theme	pH	1062:1063	arg1	concentration					1106:1118	initial pollutants concentration	1087:1118	initial pollutants concentration	1087:1118	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	5	theme	pH	1062:1063	arg1	etc.					1133:1136	reusability etc.	1121:1136	reusability etc.	1121:1136	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	5	theme	pH	1062:1063	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	6	theme	Different	1015:1023	arg1	experiments					1042:1052	Different batch adsorption experiments	1015:1052	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc.	1015:1136	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	6	theme	Different	1015:1023	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	2	7	theme	organic	502:508	arg1	pollutants					510:519	organic pollutants	502:519	organic pollutants	502:519	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	3	8	theme	surface	680:686	arg1	area					688:691	surface area	680:691	surface area	680:691	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	2	9	theme	Ethylenediaminetetraacetic	312:337	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	9	theme	Ethylenediaminetetraacetic	312:337	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	4	10	theme	adsorption	1031:1040	arg1	experiments					1042:1052	Different batch adsorption experiments	1015:1052	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc.	1015:1136	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	10	theme	adsorption	1031:1040	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	10	11	theme	toxic	2255:2259	arg1	pollutants					2261:2270	both inorganic and organic toxic pollutants	2228:2270	both inorganic and organic toxic pollutants	2228:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	4	12	theme	initial	1087:1093	arg1	concentration					1106:1118	initial pollutants concentration	1087:1118	initial pollutants concentration	1087:1118	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	12	theme	initial	1087:1093	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	2	13	from	wastewater	578:587	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	13	from	wastewater	578:587	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	8	14	theme	excellent	1657:1665	arg1	capacity					1678:1685	The excellent adsorption capacity	1653:1685	The excellent adsorption capacity	1653:1685	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	2	15	theme	blue	538:541	arg1	methylene					528:536	methylene blue	528:541	methylene blue (MB)	528:546	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	15	theme	blue	538:541	arg1	MB					544:545	MB	544:545	MB	544:545	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	5	16	theme	adsorption	1204:1213	arg1	process					1215:1221	The adsorption process	1200:1221	The adsorption process	1200:1221	The adsorption process apparently followed pseudo-second-order (PSO) kinetics for both pollutants, however the adsorption kinetics was also explained by the intra-particle diffusion model.					
34597635	2	17	theme	current	278:284	arg1	work					286:289	the current work	274:289	the current work	274:289	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	7	18	theme	adsorbent	1524:1532	arg1	capacities					1506:1515	The maximum adsorption capacities	1483:1515	The maximum adsorption capacities of the adsorbent	1483:1532	The maximum adsorption capacities of the adsorbent were determined 324 ± 3.30 130 ± 2.80, 141 ± 6.60, and 121 ± 3.50 mg g-1 for Hg(II), Cu(II), MB, and CV, respectively.					
34597635	9	19	theme	FTIR	1838:1841	arg1	analysis					1843:1850	The EDS, elemental mapping and FTIR analysis	1807:1850	analysis	1843:1850	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	3	20	theme	nanocomposite	735:747	arg1	compositions					666:677	The structural, functional, morphological, elemental compositions	613:677	compositions	666:677	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	20	theme	nanocomposite	735:747	arg1	area					688:691	surface area	680:691	surface area	680:691	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	20	theme	nanocomposite	735:747	arg1	properties					705:714	thermal properties	697:714	thermal properties of the synthesized nanocomposite	697:747	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	0	21	theme	inorganic	99:107	arg1	pollutants					121:130	inorganic and organic pollutants	99:130	inorganic and organic pollutants	99:130	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	1	22	theme	pollutants	194:203	arg1	Discharging					157:167	Discharging	157:167	Discharging of inorganic and organic pollutants	157:203	Discharging of inorganic and organic pollutants creates a serious threat to the human health and the environment.					
34597635	7	23	theme	adsorption	1495:1504	arg1	capacities					1506:1515	The maximum adsorption capacities	1483:1515	The maximum adsorption capacities of the adsorbent	1483:1532	The maximum adsorption capacities of the adsorbent were determined 324 ± 3.30 130 ± 2.80, 141 ± 6.60, and 121 ± 3.50 mg g-1 for Hg(II), Cu(II), MB, and CV, respectively.					
34597635	8	24	dep	-COOH	1765:1769	arg1	e.g.					1759:1762	e.g.	1759:1762	e.g.	1759:1762	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	3	25	theme	powder	771:776	arg1	XRD					797:799	XRD	797:799	XRD	797:799	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	25	theme	powder	771:776	arg1	diffraction					784:794	powder X-ray diffraction	771:794	powder X-ray diffraction (XRD)	771:800	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	0	26	theme	organic	113:119	arg1	pollutants					121:130	inorganic and organic pollutants	99:130	inorganic and organic pollutants	99:130	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	10	27	theme	organic	2073:2079	arg1	pollutants					2081:2090	both inorganic and organic pollutants	2054:2090	both inorganic and organic pollutants	2054:2090	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	8	28	theme	active	1733:1738	arg1	groups					1751:1756	various active functional groups	1725:1756	various active functional groups (e.g., -COOH, -OH, -NH2, etc.)	1725:1787	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	4	29	theme	reusability	1121:1131	arg1	etc.					1133:1136	reusability etc.	1121:1136	reusability etc.	1121:1136	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	29	theme	reusability	1121:1131	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	2	30	theme	simultaneous	421:432	arg1	removal					434:440	simultaneous removal	421:440	simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV))	421:571	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	31	dep	Hg	471:472	arg1	pollutants					510:519	organic pollutants	502:519	organic pollutants	502:519	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	31	dep	Hg	471:472	arg1	Cu					490:491	Cu(II)	490:495	Cu(II)	490:495	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	31	dep	Hg	471:472	arg1	II					474:475	II	474:475	II	474:475	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	31	dep	Hg	471:472	arg1	copper					482:487	copper	482:487	copper (Cu(II))	482:496	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	8	32	theme	groups	1751:1756	arg1	availability					1709:1720	the availability	1705:1720	the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent	1705:1804	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	9	33	theme	metals	1903:1908	arg1	adsorption					1883:1892	the adsorption	1879:1892	the adsorption of heavy metals and dyes by GO-EDTA-CS	1879:1931	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	5	34	theme	pseudo-second-order	1243:1261	arg1	kinetics					1269:1276	pseudo-second-order (PSO) kinetics	1243:1276	pseudo-second-order (PSO) kinetics	1243:1276	The adsorption process apparently followed pseudo-second-order (PSO) kinetics for both pollutants, however the adsorption kinetics was also explained by the intra-particle diffusion model.					
34597635	0	35	theme	EDTA-functionalized	13:31	arg1	oxide-chitosan					42:55	EDTA-functionalized graphene oxide-chitosan	13:55	EDTA-functionalized graphene oxide-chitosan	13:55	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	3	36	theme	scanning	857:864	arg1	FESEM					887:891	FESEM	887:891	FESEM	887:891	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	36	theme	scanning	857:864	arg1	microscopy					875:884	field scanning electron microscopy	851:884	field scanning electron microscopy (FESEM)	851:892	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	9	37	theme	dyes	1914:1917	arg1	adsorption					1883:1892	the adsorption	1879:1892	the adsorption of heavy metals and dyes by GO-EDTA-CS	1879:1931	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	5	38	theme	PSO	1264:1266	arg1	kinetics					1269:1276	pseudo-second-order (PSO) kinetics	1243:1276	pseudo-second-order (PSO) kinetics	1243:1276	The adsorption process apparently followed pseudo-second-order (PSO) kinetics for both pollutants, however the adsorption kinetics was also explained by the intra-particle diffusion model.					
34597635	0	39	theme	oxide-chitosan	42:55	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of EDTA-functionalized graphene oxide-chitosan	0:55	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	2	40	theme	oxide-chitosan	375:388	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	40	theme	oxide-chitosan	375:388	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	3	41	theme	structural	617:626	arg1	compositions					666:677	The structural, functional, morphological, elemental compositions	613:677	compositions	666:677	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	2	42	theme	functionalized	351:364	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	42	theme	functionalized	351:364	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	6	43	theme	metals	1416:1421	arg1	ions					1423:1426	metals ions	1416:1426	metals ions	1416:1426	The isotherm data for both metals ions and dyes were well fit by the Langmuir isotherm model.					
34597635	2	44	dep	methylene	528:536	arg1	i.e.					522:525	i.e.	522:525	i.e.	522:525	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	3	45	theme	dispersive	902:911	arg1	spectroscopy					913:924	energy dispersive spectroscopy	895:924	energy dispersive spectroscopy (EDS)	895:930	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	45	theme	dispersive	902:911	arg1	EDS					927:929	EDS	927:929	EDS	927:929	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	0	46	from	wastewater	145:154	arg1	removal					88:94	simultaneous removal	75:94	simultaneous removal of inorganic and organic pollutants from complex wastewater	75:154	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	2	47	theme	crystal	552:558	arg1	CV					568:569	CV	568:569	CV	568:569	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	47	theme	crystal	552:558	arg1	violet					560:565	crystal violet	552:565	crystal violet (CV)	552:570	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	3	48	theme	functional	629:638	arg1	compositions					666:677	The structural, functional, morphological, elemental compositions	613:677	compositions	666:677	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	6	49	theme	isotherm	1393:1400	arg1	fit					1447:1449	fit	1447:1449	fit	1447:1449	The isotherm data for both metals ions and dyes were well fit by the Langmuir isotherm model.					
34597635	6	49	theme	isotherm	1393:1400	arg1	data					1402:1405	The isotherm data	1389:1405	The isotherm data for both metals ions and dyes	1389:1435	The isotherm data for both metals ions and dyes were well fit by the Langmuir isotherm model.					
34597635	6	50	theme	isotherm	1467:1474	arg1	model					1476:1480	the Langmuir isotherm model	1454:1480	the Langmuir isotherm model	1454:1480	The isotherm data for both metals ions and dyes were well fit by the Langmuir isotherm model.					
34597635	3	51	theme	elemental	656:664	arg1	compositions					666:677	The structural, functional, morphological, elemental compositions	613:677	compositions	666:677	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	2	52	theme	EDTA	345:348	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	52	theme	EDTA	345:348	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	1	53	theme	serious	215:221	arg1	threat					223:228	a serious threat	213:228	a serious threat to the human health and the environment	213:268	Discharging of inorganic and organic pollutants creates a serious threat to the human health and the environment.					
34597635	4	54	theme	batch	1025:1029	arg1	experiments					1042:1052	Different batch adsorption experiments	1015:1052	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc.	1015:1136	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	54	theme	batch	1025:1029	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	3	55	theme	thermal	697:703	arg1	properties					705:714	thermal properties	697:714	thermal properties of the synthesized nanocomposite	697:747	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	5	56	theme	intra-particle	1357:1370	arg1	model					1382:1386	the intra-particle diffusion model	1353:1386	the intra-particle diffusion model	1353:1386	The adsorption process apparently followed pseudo-second-order (PSO) kinetics for both pollutants, however the adsorption kinetics was also explained by the intra-particle diffusion model.					
34597635	2	57	theme	acid	339:342	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	57	theme	acid	339:342	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	4	58	theme	monocomponent	1154:1166	arg1	system					1168:1173	monocomponent system	1154:1173	monocomponent system to optimize the results	1154:1197	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	1	59	theme	human	237:241	arg1	health					243:248	the human health	233:248	the human health	233:248	Discharging of inorganic and organic pollutants creates a serious threat to the human health and the environment.					
34597635	10	60	theme	wastewater	2196:2205	arg1	treatment					2207:2215	practical wastewater treatment	2186:2215	practical wastewater treatment containing both inorganic and organic toxic pollutants	2186:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	9	61	theme	EDS	1811:1813	arg1	mapping					1826:1832	The EDS, elemental mapping and FTIR analysis	1807:1850	mapping	1826:1832	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	3	62	theme	synthesized	723:733	arg1	nanocomposite					735:747	the synthesized nanocomposite	719:747	the synthesized nanocomposite	719:747	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	10	63	dep	itself	2153:2158	arg1	adsorbent					2172:2180	adsorbent	2172:2180	adsorbent	2172:2180	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	10	64	theme	organic	2247:2253	arg1	pollutants					2261:2270	both inorganic and organic toxic pollutants	2228:2270	both inorganic and organic toxic pollutants	2228:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	9	65	theme	elemental	1816:1824	arg1	mapping					1826:1832	The EDS, elemental mapping and FTIR analysis	1807:1850	mapping	1826:1832	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	8	66	from	availability	1709:1720	arg1	adsorbent					1796:1804	adsorbent	1796:1804	adsorbent	1796:1804	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	10	67	theme	inorganic	2233:2241	arg1	pollutants					2261:2270	both inorganic and organic toxic pollutants	2228:2270	both inorganic and organic toxic pollutants	2228:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	4	68	theme	contact	1073:1079	arg1	time					1081:1084	contact time	1073:1084	contact time	1073:1084	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	68	theme	contact	1073:1079	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	10	69	contain	containing	2217:2226	arg2	pollutants					2261:2270	both inorganic and organic toxic pollutants	2228:2270	both inorganic and organic toxic pollutants	2228:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	10	69	contain	containing	2217:2226	arg1	treatment					2207:2215	practical wastewater treatment	2186:2215	practical wastewater treatment containing both inorganic and organic toxic pollutants	2186:2270	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	8	70	theme	adsorption	1667:1676	arg1	capacity					1678:1685	The excellent adsorption capacity	1653:1685	The excellent adsorption capacity	1653:1685	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	1	71	theme	inorganic	172:180	arg1	pollutants					194:203	inorganic and organic pollutants	172:203	inorganic and organic pollutants	172:203	Discharging of inorganic and organic pollutants creates a serious threat to the human health and the environment.					
34597635	1	72	theme	organic	186:192	arg1	pollutants					194:203	inorganic and organic pollutants	172:203	inorganic and organic pollutants	172:203	Discharging of inorganic and organic pollutants creates a serious threat to the human health and the environment.					
34597635	7	73	theme	maximum	1487:1493	arg1	capacities					1506:1515	The maximum adsorption capacities	1483:1515	The maximum adsorption capacities of the adsorbent	1483:1532	The maximum adsorption capacities of the adsorbent were determined 324 ± 3.30 130 ± 2.80, 141 ± 6.60, and 121 ± 3.50 mg g-1 for Hg(II), Cu(II), MB, and CV, respectively.					
34597635	2	74	theme	adsorption	593:602	arg1	process					604:610	adsorption process	593:610	adsorption process	593:610	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	0	75	theme	pollutants	121:130	arg1	removal					88:94	simultaneous removal	75:94	simultaneous removal of inorganic and organic pollutants from complex wastewater	75:154	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	3	76	theme	thermogravimetric	967:983	arg1	analyzer					985:992	thermogravimetric analyzer	967:992	thermogravimetric analyzer	967:992	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	77	theme	infrared	821:828	arg1	FTIR					844:847	FTIR	844:847	FTIR	844:847	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	77	theme	infrared	821:828	arg1	TGA					995:997	TGA	995:997	TGA	995:997	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	77	theme	infrared	821:828	arg1	spectroscopy					830:841	infrared spectroscopy	821:841	infrared spectroscopy (FTIR)	821:848	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	78	theme	X-ray	778:782	arg1	XRD					797:799	XRD	797:799	XRD	797:799	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	78	theme	X-ray	778:782	arg1	diffraction					784:794	powder X-ray diffraction	771:794	powder X-ray diffraction (XRD)	771:800	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	0	79	theme	complex	137:143	arg1	wastewater					145:154	complex wastewater	137:154	complex wastewater	137:154	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	10	80	theme	inorganic	2059:2067	arg1	pollutants					2081:2090	both inorganic and organic pollutants	2054:2090	both inorganic and organic pollutants	2054:2090	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	3	81	theme	field	851:855	arg1	FESEM					887:891	FESEM	887:891	FESEM	887:891	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	81	theme	field	851:855	arg1	microscopy					875:884	field scanning electron microscopy	851:884	field scanning electron microscopy (FESEM)	851:892	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	10	82	theme	adsorption-desorption	2119:2139	arg1	cycles					2109:2114	seven cycles	2103:2114	seven cycles of adsorption-desorption	2103:2139	Moreover, GO-EDTA-CS could maintain its adsorption capacity for both inorganic and organic pollutants even after seven cycles of adsorption-desorption, indicating itself a promising adsorbent for practical wastewater treatment containing both inorganic and organic toxic pollutants.					
34597635	8	83	theme	various	1725:1731	arg1	groups					1751:1756	various active functional groups	1725:1756	various active functional groups (e.g., -COOH, -OH, -NH2, etc.)	1725:1787	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	9	84	theme	heavy	1897:1901	arg1	metals					1903:1908	heavy metals	1897:1908	heavy metals	1897:1908	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	0	85	theme	graphene	33:40	arg1	oxide-chitosan					42:55	EDTA-functionalized graphene oxide-chitosan	13:55	EDTA-functionalized graphene oxide-chitosan	13:55	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	3	86	dep	functional	629:638	arg1	morphological					641:653	morphological	641:653	morphological	641:653	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	8	87	theme	functional	1740:1749	arg1	groups					1751:1756	various active functional groups	1725:1756	various active functional groups (e.g., -COOH, -OH, -NH2, etc.)	1725:1787	The excellent adsorption capacity was attributed to the availability of various active functional groups (e.g., -COOH, -OH, -NH2, etc.) on the adsorbent.					
34597635	2	88	theme	mercury	462:468	arg1	removal					434:440	simultaneous removal	421:440	simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV))	421:571	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	3	89	theme	electron	866:873	arg1	FESEM					887:891	FESEM	887:891	FESEM	887:891	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	89	theme	electron	866:873	arg1	microscopy					875:884	field scanning electron microscopy	851:884	field scanning electron microscopy (FESEM)	851:892	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	5	90	theme	adsorption	1311:1320	arg1	kinetics					1322:1329	the adsorption kinetics	1307:1329	the adsorption kinetics	1307:1329	The adsorption process apparently followed pseudo-second-order (PSO) kinetics for both pollutants, however the adsorption kinetics was also explained by the intra-particle diffusion model.					
34597635	2	91	theme	inorganic	445:453	arg1	Hg					471:472	Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)	471:570	Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)	471:570	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	91	theme	inorganic	445:453	arg1	mercury					462:468	mercury	462:468	mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV))	462:571	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	0	92	theme	simultaneous	75:86	arg1	removal					88:94	simultaneous removal	75:94	simultaneous removal of inorganic and organic pollutants from complex wastewater	75:154	Synthesis of EDTA-functionalized graphene oxide-chitosan nanocomposite for simultaneous removal of inorganic and organic pollutants from complex wastewater.					
34597635	3	93	theme	energy	895:900	arg1	spectroscopy					913:924	energy dispersive spectroscopy	895:924	energy dispersive spectroscopy (EDS)	895:930	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	3	93	theme	energy	895:900	arg1	EDS					927:929	EDS	927:929	EDS	927:929	The structural, functional, morphological, elemental compositions, surface area and thermal properties of the synthesized nanocomposite were identified using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and thermogravimetric analyzer (TGA), respectively.					
34597635	2	94	theme	graphene	366:373	arg1	GO-EDTA-CS					405:414	GO-EDTA-CS	405:414	GO-EDTA-CS	405:414	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	2	94	theme	graphene	366:373	arg1	nanocomposite					390:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite	312:402	Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater	312:587	In the current work, we have synthesized Ethylenediaminetetraacetic acid (EDTA) functionalized graphene oxide-chitosan nanocomposite (GO-EDTA-CS) for simultaneous removal of inorganic (i.e., mercury (Hg(II) and copper (Cu(II)) and organic pollutants (i.e., methylene blue (MB) and crystal violet (CV)) from wastewater via adsorption process.					
34597635	9	95	theme	simultaneous	1947:1958	arg1	adsorption					1960:1969	the simultaneous adsorption	1943:1969	the simultaneous adsorption of the pollutants	1943:1987	The EDS, elemental mapping and FTIR analysis performed before and after the adsorption of heavy metals and dyes by GO-EDTA-CS confirmed the simultaneous adsorption of the pollutants.					
34597635	4	96	theme	pollutants	1095:1104	arg1	concentration					1106:1118	initial pollutants concentration	1087:1118	initial pollutants concentration	1087:1118	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
34597635	4	96	theme	pollutants	1095:1104	arg1	effect					1065:1070	pH effect	1062:1070	pH effect	1062:1070	Different batch adsorption experiments such as pH effect, contact time, initial pollutants concentration, reusability etc. were studied in monocomponent system to optimize the results.					
32070497	2	0	theme	permeability	470:481	arg1	parameters					489:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters	422:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages	422:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	4	1	theme	selected	688:695	arg1	formulations					702:713	selected film formulations	688:713	selected film formulations	688:713	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	1	2	theme	biological	157:166	arg1	molecules					168:176	biological molecules	157:176	biological molecules with filmogenic morphology	157:203	Biopolymers as films are defined as materials prepared from biological molecules with filmogenic morphology that can be versatile uses.					
32070497	2	3	theme	vapor	464:468	arg1	permeability					470:481	water vapor permeability	458:481	water vapor permeability	458:481	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	1	4	with	molecules	168:176	arg1	morphology					194:203	filmogenic morphology	183:203	filmogenic morphology	183:203	Biopolymers as films are defined as materials prepared from biological molecules with filmogenic morphology that can be versatile uses.					
32070497	2	5	theme	different	504:512	arg1	stages					528:533	different cross-linking stages	504:533	different cross-linking stages	504:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	2	6	theme	composites	303:312	arg1	formulations					269:280	formulations	269:280	formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol	269:375	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	4	7	with	4.0g	803:806	arg1	3g					771:772	3g	771:772	3g of BCM	771:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	8	theme	promising	833:841	arg1	results					843:849	the most promising results	824:849	the most promising results	824:849	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	2	9	theme	polymeric	293:301	arg1	composites					303:312	natural polymeric composites	285:312	natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol	285:375	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	5	10	dep	sturdy	1028:1033	arg1	improved					1084:1091	improved	1084:1091	improved by second cross-linking applied	1084:1123	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	2	11	theme	natural	285:291	arg1	composites					303:312	natural polymeric composites	285:312	natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol	285:375	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	3	12	dep	lower	593:597	arg1	WVP					626:628	WVP	626:628	WVP	626:628	After a second cross-linking was applied, they presented lower thickness, solubility, and WVP values than first cross-linking.					
32070497	3	12	dep	lower	593:597	arg1	thickness					599:607	thickness	599:607	thickness	599:607	After a second cross-linking was applied, they presented lower thickness, solubility, and WVP values than first cross-linking.					
32070497	3	12	dep	lower	593:597	arg1	solubility					610:619	solubility	610:619	solubility	610:619	After a second cross-linking was applied, they presented lower thickness, solubility, and WVP values than first cross-linking.					
32070497	2	13	theme	water	458:462	arg1	permeability					470:481	water vapor permeability	458:481	water vapor permeability	458:481	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	4	14	theme	mL	763:764	arg1	4.0g					803:806	4.0g	803:806	4.0g of glycerol	803:818	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	14	theme	mL	763:764	arg1	solutions					746:754	solutions	746:754	solutions of 400 mL with 3g of BCM	746:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	14	theme	mL	763:764	arg1	7.5g					782:785	7.5g	782:785	7.5g of alginate	782:797	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	1	15	theme	filmogenic	183:192	arg1	morphology					194:203	filmogenic morphology	183:203	filmogenic morphology	183:203	Biopolymers as films are defined as materials prepared from biological molecules with filmogenic morphology that can be versatile uses.					
32070497	2	16	theme	solubility	432:441	arg1	parameters					489:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters	422:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages	422:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	0	17	theme	coconut	79:85	arg1	mesocarp					87:94	coconut mesocarp	79:94	coconut mesocarp	79:94	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	2	18	theme	coconut	331:337	arg1	BCM					349:351	BCM	349:351	BCM	349:351	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	2	18	theme	coconut	331:337	arg1	mesocarp					339:346	babassu coconut mesocarp	323:346	babassu coconut mesocarp (BCM)	323:352	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	4	19	theme	glycerol	811:818	arg1	4.0g					803:806	4.0g	803:806	4.0g of glycerol	803:818	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	19	theme	glycerol	811:818	arg1	solutions					746:754	solutions	746:754	solutions of 400 mL with 3g of BCM	746:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	19	theme	glycerol	811:818	arg1	7.5g					782:785	7.5g	782:785	7.5g of alginate	782:797	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	2	20	theme	babassu	323:329	arg1	BCM					349:351	BCM	349:351	BCM	349:351	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	2	20	theme	babassu	323:329	arg1	mesocarp					339:346	babassu coconut mesocarp	323:346	babassu coconut mesocarp (BCM)	323:352	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	2	21	theme	moisture	422:429	arg1	parameters					489:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters	422:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages	422:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	5	22	theme	coconut	976:982	arg1	mesocarp					984:991	babassu coconut mesocarp	968:991	babassu coconut mesocarp	968:991	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	4	23	theme	thermal	893:899	arg1	chemical					911:918	chemical	911:918	chemical	911:918	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	23	theme	thermal	893:899	arg1	properties					935:944	mechanical properties	924:944	mechanical properties	924:944	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	23	theme	thermal	893:899	arg1	analyses					901:908	thermal analyses	893:908	thermal analyses	893:908	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	2	24	theme	components	408:417	arg1	effect					392:397	the effect	388:397	the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages	388:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	3	25	theme	second	544:549	arg1	cross-linking					551:563	a second cross-linking	542:563	a second cross-linking	542:563	After a second cross-linking was applied, they presented lower thickness, solubility, and WVP values than first cross-linking.					
32070497	0	26	theme	films	47:51	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	0	26	theme	films	47:51	arg1	Elaboration					0:10	Elaboration	0:10	Elaboration	0:10	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	5	27	from	Films	947:951	arg1	proportion					1000:1009	the proportion	996:1009	the proportion established	996:1021	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	0	28	dep	alginate	58:65	arg1	mesocarp					87:94	coconut mesocarp	79:94	coconut mesocarp	79:94	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	0	29	theme	biopolymer	36:45	arg1	films					47:51	biopolymer films	36:51	biopolymer films	36:51	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	4	30	with	solutions	746:754	arg1	3g					771:772	3g	771:772	3g of BCM	771:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	2	31	theme	cross-linking	514:526	arg1	stages					528:533	different cross-linking stages	504:533	different cross-linking stages	504:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	2	32	theme	present	237:243	arg1	research					245:252	The present research	233:252	The present research	233:252	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	4	33	theme	mechanical	924:933	arg1	properties					935:944	mechanical properties	924:944	mechanical properties	924:944	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	33	theme	mechanical	924:933	arg1	analyses					901:908	thermal analyses	893:908	thermal analyses	893:908	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	2	34	theme	thickness	444:452	arg1	parameters					489:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters	422:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages	422:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	5	35	theme	thermal	1063:1069	arg1	degradation					1071:1081	thermal degradation	1063:1081	thermal degradation	1063:1081	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	0	36	with	characterization	16:31	arg1	babassu					71:77	babassu	71:77	babassu	71:77	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	0	36	with	characterization	16:31	arg1	alginate					58:65	alginate	58:65	alginate	58:65	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	5	37	with	Films	947:951	arg1	mesocarp					984:991	babassu coconut mesocarp	968:991	babassu coconut mesocarp	968:991	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	5	37	with	Films	947:951	arg1	amount					958:963	amount	958:963	amount of babassu coconut mesocarp	958:991	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	4	38	theme	alginate	790:797	arg1	4.0g					803:806	4.0g	803:806	4.0g of glycerol	803:818	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	38	theme	alginate	790:797	arg1	solutions					746:754	solutions	746:754	solutions of 400 mL with 3g of BCM	746:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	38	theme	alginate	790:797	arg1	7.5g					782:785	7.5g	782:785	7.5g of alginate	782:797	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	39	theme	film	697:700	arg1	formulations					702:713	selected film formulations	688:713	selected film formulations	688:713	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	5	40	theme	mesocarp	984:991	arg1	mesocarp					984:991	babassu coconut mesocarp	968:991	babassu coconut mesocarp	968:991	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	5	40	theme	mesocarp	984:991	arg1	amount					958:963	amount	958:963	amount of babassu coconut mesocarp	958:991	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	1	41	dep	versatile	217:225	arg1	uses					227:230	uses	227:230	uses	227:230	Biopolymers as films are defined as materials prepared from biological molecules with filmogenic morphology that can be versatile uses.					
32070497	0	42	with	Elaboration	0:10	arg1	babassu					71:77	babassu	71:77	babassu	71:77	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	0	42	with	Elaboration	0:10	arg1	alginate					58:65	alginate	58:65	alginate	58:65	Elaboration and characterization of biopolymer films with alginate and babassu coconut mesocarp.					
32070497	4	43	contain	had	820:822	arg2	results					843:849	the most promising results	824:849	the most promising results	824:849	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	43	contain	had	820:822	arg1	formulation					731:741	the formulation	727:741	the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol	727:818	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	44	theme	physical	868:875	arg1	parameters					877:886	physical parameters	868:886	physical parameters	868:886	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	5	45	theme	babassu	968:974	arg1	mesocarp					984:991	babassu coconut mesocarp	968:991	babassu coconut mesocarp	968:991	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	3	46	theme	first	642:646	arg1	cross-linking					648:660	first cross-linking	642:660	first cross-linking	642:660	After a second cross-linking was applied, they presented lower thickness, solubility, and WVP values than first cross-linking.					
32070497	3	47	theme	lower	593:597	arg1	values					630:635	lower thickness, solubility, and WVP values	593:635	lower thickness, solubility, and WVP values	593:635	After a second cross-linking was applied, they presented lower thickness, solubility, and WVP values than first cross-linking.					
32070497	2	48	from	effect	392:397	arg1	parameters					489:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters	422:498	moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages	422:533	The present research aimed to study formulations of natural polymeric composites based on babassu coconut mesocarp (BCM), alginate and glycerol, to verify the effect of these components on moisture, solubility, thickness and water vapor permeability (WVP) parameters for different cross-linking stages.					
32070497	5	49	theme	second	1096:1101	arg1	cross-linking					1103:1115	second cross-linking	1096:1115	second cross-linking applied	1096:1123	Films with amount of babassu coconut mesocarp in the proportion established were sturdy to solubility, leaching and thermal degradation, improved by second cross-linking applied.					
32070497	4	50	theme	BCM	777:779	arg1	3g					771:772	3g	771:772	3g of BCM	771:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	51	theme	Consecutive	663:673	arg1	analyses					675:682	Consecutive analyses	663:682	Consecutive analyses for selected film formulations	663:713	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32070497	4	52	with	7.5g	782:785	arg1	3g					771:772	3g	771:772	3g of BCM	771:779	Consecutive analyses for selected film formulations showed that the formulation to solutions of 400 mL with 3g of BCM, 7.5g of alginate and 4.0g of glycerol had the most promising results when correlating physical parameters with thermal analyses, chemical and mechanical properties.					
32172891	6	0	theme	increased	933:941	arg1	elasticity					943:952	increased elasticity	933:952	increased elasticity	933:952	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	1	1	theme	carboxymethyl	175:187	arg1	CMCS					199:202	CMCS	199:202	CMCS	199:202	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	1	1	theme	carboxymethyl	175:187	arg1	chitosan					189:196	carboxymethyl chitosan	175:196	carboxymethyl chitosan (CMCS)	175:203	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	4	2	theme	biopolymer	703:712	arg1	films					714:718	CMCS-based biopolymer films	692:718	CMCS-based biopolymer films	692:718	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	4	2	theme	biopolymer	703:712	arg1	packagings					728:737	food packagings	723:737	food packagings	723:737	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	4	3	theme	water-rich	766:775	arg1	food					777:780	water-rich food	766:780	water-rich food	766:780	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	4	4	theme	UV	600:601	arg1	performance					613:623	better UV shielding performance	593:623	better UV shielding performance	593:623	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	0	5	theme	food	114:117	arg1	packaging					119:127	food packaging	114:127	food packaging	114:127	MgO/carboxymethyl chitosan nanocomposite improves thermal stability, waterproof and antibacterial performance for food packaging.					
32172891	7	6	theme	Shewanella	1143:1152	arg1	baltica					1154:1160	Shewanella baltica	1143:1160	Shewanella baltica	1143:1160	For biological properties, CMCS/MgO composites exhibited excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica.					
32172891	3	7	theme	crystallinity	498:510	arg1	improvement					483:493	the improvement	479:493	the improvement of crystallinity	479:510	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	4	8	theme	better	593:598	arg1	performance					613:623	better UV shielding performance	593:623	better UV shielding performance	593:623	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	4	9	theme	food	723:726	arg1	films					714:718	CMCS-based biopolymer films	692:718	CMCS-based biopolymer films	692:718	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	4	9	theme	food	723:726	arg1	packagings					728:737	food packagings	723:737	food packagings	723:737	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	7	10	theme	Listeria	1116:1123	arg1	monocytogenes					1125:1137	Listeria monocytogenes	1116:1137	Listeria monocytogenes	1116:1137	For biological properties, CMCS/MgO composites exhibited excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica.					
32172891	3	11	theme	SEM	371:373	arg1	micrographs					375:385	SEM micrographs	371:385	SEM micrographs	371:385	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	3	12	from	layer	404:408	arg1	matrix					439:444	the CMCS matrix	430:444	the CMCS matrix	430:444	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	3	12	from	layer	404:408	arg1	contributor					464:474	a major contributor	456:474	a major contributor to the improvement of crystallinity	456:510	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	2	13	theme	nanoparticles	285:297	arg1	impregnation					265:276	The impregnation	261:276	The impregnation of MgO nanoparticles into CMCS	261:307	The impregnation of MgO nanoparticles into CMCS was evidenced by the X-ray diffraction and FTIR spectroscopy.					
32172891	0	14	theme	chitosan	18:25	arg1	nanocomposite					27:39	MgO/carboxymethyl chitosan nanocomposite	0:39	MgO/carboxymethyl chitosan nanocomposite	0:39	MgO/carboxymethyl chitosan nanocomposite improves thermal stability, waterproof and antibacterial performance for food packaging.					
32172891	4	15	theme	improved	565:572	arg1	stability					582:590	improved thermal stability	565:590	improved thermal stability	565:590	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	2	16	theme	X-ray	330:334	arg1	diffraction					336:346	X-ray diffraction	330:346	X-ray diffraction	330:346	The impregnation of MgO nanoparticles into CMCS was evidenced by the X-ray diffraction and FTIR spectroscopy.					
32172891	3	17	theme	CMCS	434:437	arg1	matrix					439:444	the CMCS matrix	430:444	the CMCS matrix	430:444	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	3	17	theme	CMCS	434:437	arg1	contributor					464:474	a major contributor	456:474	a major contributor to the improvement of crystallinity	456:510	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	0	18	theme	MgO/carboxymethyl	0:16	arg1	nanocomposite					27:39	MgO/carboxymethyl chitosan nanocomposite	0:39	MgO/carboxymethyl chitosan nanocomposite	0:39	MgO/carboxymethyl chitosan nanocomposite improves thermal stability, waterproof and antibacterial performance for food packaging.					
32172891	2	19	dep	diffraction	336:346	arg1	the					326:328	the	326:328	the	326:328	The impregnation of MgO nanoparticles into CMCS was evidenced by the X-ray diffraction and FTIR spectroscopy.					
32172891	7	20	theme	excellent	1075:1083	arg1	activity					1099:1106	excellent antimicrobial activity	1075:1106	excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica	1075:1160	For biological properties, CMCS/MgO composites exhibited excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica.					
32172891	4	21	theme	shielding	603:611	arg1	performance					613:623	better UV shielding performance	593:623	better UV shielding performance	593:623	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	6	22	theme	filler	984:989	arg1	content					991:997	a rather low filler content	971:997	a rather low filler content (1.0 % by weight)	971:1015	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	6	23	theme	low	980:982	arg1	content					991:997	a rather low filler content	971:997	a rather low filler content (1.0 % by weight)	971:1015	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	1	24	theme	nano	209:212	arg1	MgO					214:216	nano MgO	209:216	nano MgO for potential food packaging applications	209:258	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	3	25	theme	dense	398:402	arg1	layer					404:408	a dense layer	396:408	a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity	396:510	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	4	26	theme	thermal	574:580	arg1	stability					582:590	improved thermal stability	565:590	improved thermal stability	565:590	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	7	27	theme	antimicrobial	1085:1097	arg1	activity					1099:1106	excellent antimicrobial activity	1075:1106	excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica	1075:1160	For biological properties, CMCS/MgO composites exhibited excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica.					
32172891	6	28	theme	MgO	878:880	arg1	nanoparticles					882:894	MgO nanoparticles	878:894	MgO nanoparticles	878:894	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	2	29	theme	FTIR	352:355	arg1	spectroscopy					357:368	FTIR spectroscopy	352:368	FTIR spectroscopy	352:368	The impregnation of MgO nanoparticles into CMCS was evidenced by the X-ray diffraction and FTIR spectroscopy.					
32172891	6	30	from	content	991:997	arg1	CMCS					923:926	CMCS	923:926	CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight)	923:1015	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	5	31	theme	MgO	852:854	arg1	content					856:862	MgO content	852:862	MgO content	852:862	These physical properties were further enhanced with the increase in MgO content.					
32172891	0	32	theme	thermal	50:56	arg1	stability					58:66	thermal stability	50:66	thermal stability	50:66	MgO/carboxymethyl chitosan nanocomposite improves thermal stability, waterproof and antibacterial performance for food packaging.					
32172891	5	33	theme	physical	789:796	arg1	properties					798:807	These physical properties	783:807	These physical properties	783:807	These physical properties were further enhanced with the increase in MgO content.					
32172891	1	34	theme	potential	222:230	arg1	applications					247:258	potential food packaging applications	222:258	potential food packaging applications	222:258	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	3	35	theme	MgO	413:415	arg1	formation					417:425	MgO formation	413:425	MgO formation	413:425	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	4	36	theme	pure	527:530	arg1	CMCS					532:535	pure CMCS	527:535	pure CMCS	527:535	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	7	37	theme	biological	1022:1031	arg1	properties					1033:1042	biological properties	1022:1042	biological properties	1022:1042	For biological properties, CMCS/MgO composites exhibited excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica.					
32172891	1	38	theme	novel	132:136	arg1	film					152:155	A novel nanocomposite film	130:155	A novel nanocomposite film	130:155	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	3	39	theme	formation	417:425	arg1	layer					404:408	a dense layer	396:408	a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity	396:510	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	4	40	theme	CMCS/MgO	538:545	arg1	composites					547:556	CMCS/MgO composites	538:556	CMCS/MgO composites	538:556	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	7	41	theme	CMCS/MgO	1045:1052	arg1	composites					1054:1063	CMCS/MgO composites	1045:1063	CMCS/MgO composites	1045:1063	For biological properties, CMCS/MgO composites exhibited excellent antimicrobial activity against Listeria monocytogenes and Shewanella baltica.					
32172891	5	42	from	increase	840:847	arg1	content					856:862	MgO content	852:862	MgO content	852:862	These physical properties were further enhanced with the increase in MgO content.					
32172891	1	43	theme	nanocomposite	138:150	arg1	film					152:155	A novel nanocomposite film	130:155	A novel nanocomposite film	130:155	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	6	44	with	CMCS	923:926	arg1	ductility					958:966	ductility	958:966	ductility	958:966	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	6	44	with	CMCS	923:926	arg1	elasticity					943:952	increased elasticity	933:952	increased elasticity	933:952	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	2	45	theme	MgO	281:283	arg1	nanoparticles					285:297	MgO nanoparticles	281:297	MgO nanoparticles	281:297	The impregnation of MgO nanoparticles into CMCS was evidenced by the X-ray diffraction and FTIR spectroscopy.					
32172891	1	46	theme	food	232:235	arg1	applications					247:258	potential food packaging applications	222:258	potential food packaging applications	222:258	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	4	47	theme	food	777:780	arg1	case					758:761	the case	754:761	the case of water-rich food	754:780	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	6	48	dep	content	991:997	arg1	%					1004:1004	1.0 %	1000:1004	1.0 %	1000:1004	Furthermore, MgO nanoparticles can simultaneously provide CMCS with increased elasticity and ductility at a rather low filler content (1.0 % by weight).					
32172891	1	49	theme	packaging	237:245	arg1	applications					247:258	potential food packaging applications	222:258	potential food packaging applications	222:258	A novel nanocomposite film was fabricated by carboxymethyl chitosan (CMCS) and nano MgO for potential food packaging applications.					
32172891	0	50	theme	antibacterial	84:96	arg1	performance					98:108	antibacterial performance	84:108	antibacterial performance	84:108	MgO/carboxymethyl chitosan nanocomposite improves thermal stability, waterproof and antibacterial performance for food packaging.					
32172891	3	51	theme	major	458:462	arg1	matrix					439:444	the CMCS matrix	430:444	the CMCS matrix	430:444	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	3	51	theme	major	458:462	arg1	contributor					464:474	a major contributor	456:474	a major contributor to the improvement of crystallinity	456:510	SEM micrographs revealed a dense layer of MgO formation in the CMCS matrix, which is a major contributor to the improvement of crystallinity.					
32172891	4	52	theme	CMCS-based	692:701	arg1	films					714:718	CMCS-based biopolymer films	692:718	CMCS-based biopolymer films	692:718	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
32172891	4	52	theme	CMCS-based	692:701	arg1	packagings					728:737	food packagings	723:737	food packagings	723:737	Compared with pure CMCS, CMCS/MgO composites confer improved thermal stability, better UV shielding performance, as well as water-insolubility, improving the feasibility of using CMCS-based biopolymer films as food packagings, especially in the case of water-rich food.					
34478956	1	0	theme	antibacterial	275:287	arg1	nanoparticle					150:161	Fe3O4 nanoparticle	144:161	Fe3O4 nanoparticle loaded with silver ion	144:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	1	0	theme	antibacterial	275:287	arg1	nanomaterial					289:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	6	1	theme	@	866:866	arg1	NH2-Ag					867:872	Fe3O4@NH2-Ag	861:872	Fe3O4@NH2-Ag	861:872	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	8	2	theme	±	1419:1419	arg1	%					1425:1425	3.3 ± 1.5 %	1415:1425	3.3 ± 1.5 %	1415:1425	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	7	3	theme	Fe3O4	1037:1041	arg1	addition					1025:1032	the addition	1021:1032	the addition of Fe3O4	1021:1041	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	5	4	used	used	782:785	arg2	Fe3O4-Ag					719:726	Fe3O4-Ag	719:726	Fe3O4-Ag	719:726	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	4	used	used	782:785	arg2	ion					741:743	silver ion	734:743	silver ion	734:743	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	4	used	used	782:785	arg2	Fe3O4					712:716	silver ion loaded Fe3O4	694:716	silver ion loaded Fe3O4 (Fe3O4-Ag)	694:727	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	4	used	used	782:785	arg2	Fe3O4NP					685:691	Pristine Fe3O4NP	676:691	Pristine Fe3O4NP	676:691	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	2	5	theme	3-aminopropyl	345:357	arg1	APTMS					377:381	APTMS	377:381	APTMS	377:381	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	2	5	theme	3-aminopropyl	345:357	arg1	trimethoxysilane					359:374	3-aminopropyl trimethoxysilane	345:374	3-aminopropyl trimethoxysilane (APTMS)	345:382	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	7	6	theme	±	1246:1246	arg1	°					1252:1252	56.9 ± 4.6 ° and 56.9 ± 4.6 °	1224:1252	°	1252:1252	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	4	7	theme	@	652:652	arg1	NH2					653:655	Fe3O4@NH2	647:655	Fe3O4@NH2	647:655	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	5	8	theme	loaded	705:710	arg1	Fe3O4-Ag					719:726	Fe3O4-Ag	719:726	Fe3O4-Ag	719:726	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	8	theme	loaded	705:710	arg1	Fe3O4					712:716	silver ion loaded Fe3O4	694:716	silver ion loaded Fe3O4 (Fe3O4-Ag)	694:727	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	8	9	dep	T280	1393:1396	arg1	%					1425:1425	3.3 ± 1.5 %	1415:1425	3.3 ± 1.5 %	1415:1425	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	8	9	dep	T280	1393:1396	arg1	%					1409:1409	0.1 ± 0.0 %	1399:1409	0.1 ± 0.0 %	1399:1409	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	8	10	dep	NH2-Ag	1340:1345	arg1	°C					1351:1352	22 °C vs. 13 °C	1348:1362	°C	1351:1352	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	8	10	dep	NH2-Ag	1340:1345	arg1	°C					1361:1362	22 °C vs. 13 °C	1348:1362	°C	1361:1362	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	5	11	theme	carrageenan-based	802:818	arg1	films					830:834	carrageenan-based composite films	802:834	carrageenan-based composite films	802:834	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	1	12	theme	Fe3O4	144:148	arg1	nanoparticle					150:161	Fe3O4 nanoparticle	144:161	Fe3O4 nanoparticle loaded with silver ion	144:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	1	12	theme	Fe3O4	144:148	arg1	nanomaterial					289:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	6	13	theme	antimicrobial	893:905	arg1	activity					907:914	stronger antimicrobial activity	884:914	stronger antimicrobial activity	884:914	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	7	14	from	°	1130:1130	arg1	films					1092:1096	the carrageenan films	1076:1096	the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °	1076:1130	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	7	14	from	°	1130:1130	arg1	WCA					1069:1071	the WCA	1065:1071	the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °	1065:1130	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	5	15	theme	silver	694:699	arg1	Fe3O4-Ag					719:726	Fe3O4-Ag	719:726	Fe3O4-Ag	719:726	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	15	theme	silver	694:699	arg1	Fe3O4					712:716	silver ion loaded Fe3O4	694:716	silver ion loaded Fe3O4 (Fe3O4-Ag)	694:727	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	8	16	theme	carrageenan	1317:1327	arg1	stability					1304:1312	the thermal stability	1292:1312	the thermal stability of carrageenan	1292:1327	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	1	17	theme	silver	175:180	arg1	ion					182:184	silver ion	175:184	silver ion	175:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	8	18	theme	Fe3O4	1334:1338	arg1	NH2-Ag					1340:1345	Fe3O4@NH2-Ag	1334:1345	Fe3O4@NH2-Ag (22 °C vs. 13 °C)	1334:1363	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	5	19	theme	Fe3O4	752:756	arg1	NH2-Ag					769:774	Fe3O4@NH2-Ag	763:774	Fe3O4@NH2-Ag	763:774	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	19	theme	Fe3O4	752:756	arg1	NH2					758:760	loaded Fe3O4@NH2	745:760	loaded Fe3O4@NH2 (Fe3O4@NH2-Ag)	745:775	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	4	20	theme	Energy	551:556	arg1	EDS					583:585	EDS	583:585	EDS	583:585	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	4	20	theme	Energy	551:556	arg1	spectroscopy					569:580	Energy dispersive spectroscopy	551:580	Energy dispersive spectroscopy (EDS) results	551:594	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	0	21	theme	carrageenan-based	102:118	arg1	films					137:141	carrageenan-based active packaging films	102:141	carrageenan-based active packaging films	102:141	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	6	22	dep	log	999:1001	arg1	3.93					994:997	3.93	994:997	3.93	994:997	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	6	22	dep	log	999:1001	arg1	10.09					984:988	10.09	984:988	10.09	984:988	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	2	23	theme	antibacterial	434:446	arg1	activity					448:455	antibacterial activity	434:455	antibacterial activity	434:455	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	0	24	theme	packaging	127:135	arg1	films					137:141	carrageenan-based active packaging films	102:141	carrageenan-based active packaging films	102:141	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	0	25	theme	-modified	49:57	arg1	nanoparticles					65:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	7	26	theme	lesser	1209:1214	arg1	extent					1216:1221	a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively)	1207:1267	a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively)	1207:1267	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	0	27	theme	Silver	0:5	arg1	ion					7:9	Silver ion	0:9	Silver ion	0:9	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	3	28	theme	Fe3O4	506:510	arg1	NH2					512:514	Fe3O4@NH2	506:514	Fe3O4@NH2	506:514	Silver ions were adsorbed on pristine Fe3O4 and Fe3O4@NH2 to enhance antibacterial activity.					
34478956	3	29	theme	Silver	458:463	arg1	ions					465:468	Silver ions	458:468	Silver ions	458:468	Silver ions were adsorbed on pristine Fe3O4 and Fe3O4@NH2 to enhance antibacterial activity.					
34478956	7	30	theme	5.4	1110:1112	arg1	±					1124:1124	±	1124:1124	±	1124:1124	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	0	31	theme	trimethoxysilane	32:47	arg1	nanoparticles					65:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	7	32	theme	films	1092:1096	arg1	WCA					1069:1071	the WCA	1065:1071	the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °	1065:1130	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	8	33	theme	blocking	1372:1379	arg1	properties					1381:1390	UV blocking properties	1369:1390	UV blocking properties	1369:1390	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	5	34	theme	Fe3O4	763:767	arg1	NH2-Ag					769:774	Fe3O4@NH2-Ag	763:774	Fe3O4@NH2-Ag	763:774	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	34	theme	Fe3O4	763:767	arg1	NH2					758:760	loaded Fe3O4@NH2	745:760	loaded Fe3O4@NH2 (Fe3O4@NH2-Ag)	745:775	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	8	35	dep	improved	1283:1290	arg1	T280					1393:1396	T280	1393:1396	T280	1393:1396	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	6	36	dep	log	947:949	arg1	5.02					942:945	5.02	942:945	5.02	942:945	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	6	36	dep	log	947:949	arg1	8.82					933:936	8.82	933:936	8.82	933:936	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	7	37	from	WCA	1069:1071	arg1	°					1130:1130	61.1 ± 5.4 ° to 37.2 ± 2.1 °	1103:1130	61.1 ± 5.4 ° to 37.2 ± 2.1 °	1103:1130	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	5	38	theme	@	757:757	arg1	NH2-Ag					769:774	Fe3O4@NH2-Ag	763:774	Fe3O4@NH2-Ag	763:774	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	38	theme	@	757:757	arg1	NH2					758:760	loaded Fe3O4@NH2	745:760	loaded Fe3O4@NH2 (Fe3O4@NH2-Ag)	745:775	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	7	39	theme	@	1168:1168	arg1	NH2-Ag					1169:1174	Fe3O4@NH2-Ag	1163:1174	Fe3O4@NH2-Ag	1163:1174	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	2	40	theme	ion	406:408	arg1	capacity					421:428	the silver ion adsorption capacity	395:428	the silver ion adsorption capacity	395:428	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	6	41	theme	stronger	884:891	arg1	activity					907:914	stronger antimicrobial activity	884:914	stronger antimicrobial activity	884:914	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	8	42	theme	±	1403:1403	arg1	%					1409:1409	0.1 ± 0.0 %	1399:1409	0.1 ± 0.0 %	1399:1409	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	7	43	theme	2.1	1126:1128	arg1	±					1124:1124	±	1124:1124	±	1124:1124	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	1	44	theme	silver-based	262:273	arg1	nanoparticle					150:161	Fe3O4 nanoparticle	144:161	Fe3O4 nanoparticle loaded with silver ion	144:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	1	44	theme	silver-based	262:273	arg1	nanomaterial					289:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	6	45	theme	Fe3O4	861:865	arg1	NH2-Ag					867:872	Fe3O4@NH2-Ag	861:872	Fe3O4@NH2-Ag	861:872	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	4	46	theme	wt	628:629	arg1	Ag					635:636	Ag	635:636	Ag	635:636	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	4	46	theme	wt	628:629	arg1	%					630:630	2.74 wt%	623:630	2.74 wt% of Ag	623:636	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	2	47	theme	Fe3O4	307:311	arg1	nanoparticle					313:324	The Fe3O4 nanoparticle	303:324	The Fe3O4 nanoparticle	303:324	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	5	48	theme	composite	820:828	arg1	films					830:834	carrageenan-based composite films	802:834	carrageenan-based composite films	802:834	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	0	49	theme	Fe3O4	59:63	arg1	nanoparticles					65:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	4	50	theme	9.88	666:669	arg1	wt					671:672	wt	671:672	wt	671:672	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	5	51	theme	ion	701:703	arg1	Fe3O4-Ag					719:726	Fe3O4-Ag	719:726	Fe3O4-Ag	719:726	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	51	theme	ion	701:703	arg1	Fe3O4					712:716	silver ion loaded Fe3O4	694:716	silver ion loaded Fe3O4 (Fe3O4-Ag)	694:727	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	7	52	dep	extent	1216:1221	arg1	°					1252:1252	56.9 ± 4.6 ° and 56.9 ± 4.6 °	1224:1252	°	1252:1252	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	7	52	dep	extent	1216:1221	arg1	°					1235:1235	56.9 ± 4.6 ° and 56.9 ± 4.6 °	1224:1252	°	1235:1235	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	4	53	theme	Fe3O4	647:651	arg1	NH2					653:655	Fe3O4@NH2	647:655	Fe3O4@NH2	647:655	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	1	54	theme	safer	220:224	arg1	nanoparticle					150:161	Fe3O4 nanoparticle	144:161	Fe3O4 nanoparticle loaded with silver ion	144:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	1	54	theme	safer	220:224	arg1	nanomaterial					289:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	8	55	theme	thermal	1296:1302	arg1	stability					1304:1312	the thermal stability	1292:1312	the thermal stability of carrageenan	1292:1327	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	7	56	theme	37.2	1119:1122	arg1	±					1124:1124	±	1124:1124	±	1124:1124	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	5	57	theme	Pristine	676:683	arg1	Fe3O4NP					685:691	Pristine Fe3O4NP	676:691	Pristine Fe3O4NP	676:691	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	4	58	dep	adsorbed	614:621	arg1	whereas					639:645	whereas	639:645	whereas	639:645	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	6	59	dep	reduction	951:959	arg1	log					947:949	log	947:949	log	947:949	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	7	60	theme	±	1229:1229	arg1	°					1235:1235	56.9 ± 4.6 ° and 56.9 ± 4.6 °	1224:1252	°	1235:1235	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	5	61	theme	silver	734:739	arg1	ion					741:743	silver ion	734:743	silver ion	734:743	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	1	62	theme	efficient	209:217	arg1	nanoparticle					150:161	Fe3O4 nanoparticle	144:161	Fe3O4 nanoparticle loaded with silver ion	144:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	1	62	theme	efficient	209:217	arg1	nanomaterial					289:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	8	63	theme	@	1339:1339	arg1	NH2-Ag					1340:1345	Fe3O4@NH2-Ag	1334:1345	Fe3O4@NH2-Ag (22 °C vs. 13 °C)	1334:1363	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	5	64	theme	loaded	745:750	arg1	NH2-Ag					769:774	Fe3O4@NH2-Ag	763:774	Fe3O4@NH2-Ag	763:774	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	64	theme	loaded	745:750	arg1	NH2					758:760	loaded Fe3O4@NH2	745:760	loaded Fe3O4@NH2 (Fe3O4@NH2-Ag)	745:775	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	4	65	theme	dispersive	558:567	arg1	EDS					583:585	EDS	583:585	EDS	583:585	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	4	65	theme	dispersive	558:567	arg1	spectroscopy					569:580	Energy dispersive spectroscopy	551:580	Energy dispersive spectroscopy (EDS) results	551:594	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	0	66	theme	active	120:125	arg1	films					137:141	carrageenan-based active packaging films	102:141	carrageenan-based active packaging films	102:141	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	0	67	theme	films	137:141	arg1	fabrication					87:97	the fabrication	83:97	the fabrication of carrageenan-based active packaging films	83:141	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	5	68	dep	Fe3O4NP	685:691	arg1	NH2-Ag					769:774	Fe3O4@NH2-Ag	763:774	Fe3O4@NH2-Ag	763:774	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	68	dep	Fe3O4NP	685:691	arg1	NH2					758:760	loaded Fe3O4@NH2	745:760	loaded Fe3O4@NH2 (Fe3O4@NH2-Ag)	745:775	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	3	69	theme	pristine	487:494	arg1	Fe3O4					496:500	pristine Fe3O4	487:500	pristine Fe3O4	487:500	Silver ions were adsorbed on pristine Fe3O4 and Fe3O4@NH2 to enhance antibacterial activity.					
34478956	5	70	theme	@	768:768	arg1	NH2-Ag					769:774	Fe3O4@NH2-Ag	763:774	Fe3O4@NH2-Ag	763:774	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	5	70	theme	@	768:768	arg1	NH2					758:760	loaded Fe3O4@NH2	745:760	loaded Fe3O4@NH2 (Fe3O4@NH2-Ag)	745:775	Pristine Fe3O4NP, silver ion loaded Fe3O4 (Fe3O4-Ag), and silver ion loaded Fe3O4@NH2 (Fe3O4@NH2-Ag) were used to manufacture carrageenan-based composite films.					
34478956	7	71	theme	film	1199:1202	arg1	WCA					1188:1190	the WCA	1184:1190	the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively)	1184:1267	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	6	72	dep	reduction	1003:1011	arg1	log					999:1001	log	999:1001	log	999:1001	Compared with Fe3O4-Ag, Fe3O4@NH2-Ag exhibited stronger antimicrobial activity against E. coli (8.82 vs. 5.02 log reduction) and L. monocytogenes (10.09 vs. 3.93 log reduction).					
34478956	2	73	theme	adsorption	410:419	arg1	capacity					421:428	the silver ion adsorption capacity	395:428	the silver ion adsorption capacity	395:428	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	0	74	theme	3-aminopropyl	18:30	arg1	nanoparticles					65:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles	18:77	Silver ion loaded 3-aminopropyl trimethoxysilane -modified Fe3O4 nanoparticles for the fabrication of carrageenan-based active packaging films.					
34478956	8	75	theme	UV	1369:1370	arg1	properties					1381:1390	UV blocking properties	1369:1390	UV blocking properties	1369:1390	Fe3O4NP also improved the thermal stability of carrageenan over Fe3O4@NH2-Ag (22 °C vs. 13 °C) and UV blocking properties (T280, 0.1 ± 0.0 % vs. 3.3 ± 1.5 %).					
34478956	3	76	theme	@	511:511	arg1	NH2					512:514	Fe3O4@NH2	506:514	Fe3O4@NH2	506:514	Silver ions were adsorbed on pristine Fe3O4 and Fe3O4@NH2 to enhance antibacterial activity.					
34478956	7	77	theme	carrageenan	1080:1090	arg1	films					1092:1096	the carrageenan films	1076:1096	the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °	1076:1130	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	7	78	theme	NH2-Ag	1169:1174	arg1	additions					1137:1145	the additions	1133:1145	the additions of Fe3O4-Ag and Fe3O4@NH2-Ag	1133:1174	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	3	79	theme	antibacterial	527:539	arg1	activity					541:548	antibacterial activity	527:548	antibacterial activity	527:548	Silver ions were adsorbed on pristine Fe3O4 and Fe3O4@NH2 to enhance antibacterial activity.					
34478956	7	80	theme	Fe3O4	1163:1167	arg1	NH2-Ag					1169:1174	Fe3O4@NH2-Ag	1163:1174	Fe3O4@NH2-Ag	1163:1174	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	4	81	theme	Ag	635:636	arg1	Ag					635:636	Ag	635:636	Ag	635:636	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	4	81	theme	Ag	635:636	arg1	%					630:630	2.74 wt%	623:630	2.74 wt% of Ag	623:636	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	7	82	dep	±	1124:1124	arg1	°					1114:1114	°	1114:1114	°	1114:1114	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	7	82	dep	±	1124:1124	arg1	to					1116:1117	to	1116:1117	to	1116:1117	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	2	83	theme	silver	399:404	arg1	capacity					421:428	the silver ion adsorption capacity	395:428	the silver ion adsorption capacity	395:428	The Fe3O4 nanoparticle was modified using 3-aminopropyl trimethoxysilane (APTMS) to enhance the silver ion adsorption capacity and antibacterial activity.					
34478956	4	84	theme	spectroscopy	569:580	arg1	results					588:594	Energy dispersive spectroscopy (EDS) results	551:594	Energy dispersive spectroscopy (EDS) results	551:594	Energy dispersive spectroscopy (EDS) results showed that Fe3O4 adsorbed 2.74 wt% of Ag, whereas Fe3O4@NH2 adsorbed 9.88 wt%.					
34478956	7	85	theme	Fe3O4-Ag	1150:1157	arg1	additions					1137:1145	the additions	1133:1145	the additions of Fe3O4-Ag and Fe3O4@NH2-Ag	1133:1174	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	7	86	theme	±	1108:1108	arg1	°					1130:1130	61.1 ± 5.4 ° to 37.2 ± 2.1 °	1103:1130	61.1 ± 5.4 ° to 37.2 ± 2.1 °	1103:1130	While the addition of Fe3O4 significantly reduced the WCA of the carrageenan films from 61.1 ± 5.4 ° to 37.2 ± 2.1 °, the additions of Fe3O4-Ag and Fe3O4@NH2-Ag reduced the WCA of the film to a lesser extent (56.9 ± 4.6 ° and 56.9 ± 4.6 °, respectively).					
34478956	1	87	theme	hazardous	252:260	arg1	nanoparticle					150:161	Fe3O4 nanoparticle	144:161	Fe3O4 nanoparticle loaded with silver ion	144:184	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
34478956	1	87	theme	hazardous	252:260	arg1	nanomaterial					289:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial	202:300	Fe3O4 nanoparticle loaded with silver ion was prepared as a more efficient, safer, and less environmentally hazardous silver-based antibacterial nanomaterial.					
32005783	2	0	theme	XTH9	355:358	arg1	functions					333:341	functions	333:341	functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana)	333:460	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	9	1	theme	cell	1661:1664	arg1	integrity					1671:1679	cell wall integrity	1661:1679	cell wall integrity signaling	1661:1689	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	7	2	from	changes	1252:1258	arg1	cellulose					1274:1282	cellulose	1274:1282	cellulose	1274:1282	These changes correlated with specific Raman signals from the walls, indicating changes in lignin and cellulose.					
32005783	7	2	from	changes	1252:1258	arg1	lignin					1263:1268	lignin	1263:1268	lignin	1263:1268	These changes correlated with specific Raman signals from the walls, indicating changes in lignin and cellulose.					
32005783	4	3	theme	wall	622:625	arg1	thickening					627:636	secondary wall thickening	612:636	secondary wall thickening	612:636	They also stimulated secondary wall thickening but reduced secondary xylem production.					
32005783	5	4	theme	inflorescence	700:712	arg1	stems					714:718	inflorescence stems	700:718	inflorescence stems	700:718	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	3	5	theme	fiber	567:571	arg1	growth					583:588	fiber intrusive growth	567:588	fiber intrusive growth	567:588	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	5	6	theme	secondary	938:946	arg1	walls					948:952	secondary walls	938:952	secondary walls were thinner	938:965	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	9	7	theme	integrity	1671:1679	arg1	signaling					1681:1689	cell wall integrity signaling	1661:1689	cell wall integrity signaling	1661:1689	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	10	8	theme	wall	1937:1940	arg1	integrity					1942:1950	cell wall integrity	1932:1950	cell wall integrity sensing	1932:1958	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	6	9	theme	layers	1164:1169	arg1	number					1154:1159	an intermediate number	1138:1159	an intermediate number of layers	1138:1169	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	5	10	theme	Cell	678:681	arg1	analyses					688:695	Cell wall analyses	678:695	Cell wall analyses of inflorescence stems	678:718	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	10	11	theme	Prominent	1785:1793	arg1	effects					1795:1801	Prominent effects	1785:1801	Prominent effects of XTH4 and XTH9 on secondary xylem	1785:1837	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	9	12	theme	secondary	1463:1471	arg1	biosynthesis					1478:1489	secondary wall biosynthesis	1463:1489	secondary wall biosynthesis	1463:1489	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	2	13	theme	function	304:311	arg1	approaches					313:322	loss- and gain-of function approaches	286:322	loss- and gain-of function approaches	286:322	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	8	14	theme	Secondary	1285:1293	arg1	walls					1295:1299	Secondary walls	1285:1299	Secondary walls	1285:1299	Secondary walls were affected also in the interfascicular fibers, where neither XTH4 nor XTH9 was expressed, indicating that these effects were indirect.					
32005783	5	15	theme	xylem	886:890	arg1	production					892:901	higher xylem production	879:901	higher xylem production	879:901	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	1	16	theme	major	108:112	arg1	hemicellulose					114:126	the major hemicellulose	104:126	the major hemicellulose of dicotyledon primary cell walls	104:160	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	1	16	theme	major	108:112	arg1	Xyloglucan					90:99	Xyloglucan	90:99	Xyloglucan	90:99	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	9	17	theme	cell	1728:1731	arg1	production					1733:1742	xylem cell production	1722:1742	xylem cell production	1722:1742	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	6	18	theme	wild	1042:1045	arg1	type					1047:1050	the wild type	1038:1050	the wild type	1038:1050	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	1	19	theme	primary	143:149	arg1	walls					156:160	dicotyledon primary cell walls	131:160	dicotyledon primary cell walls	131:160	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	9	20	theme	secondary	1758:1766	arg1	thickening					1773:1782	secondary wall thickening	1758:1782	secondary wall thickening	1758:1782	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	0	21	theme	Secondary	64:72	arg1	Formation					79:87	Secondary Wall Formation	64:87	Secondary Wall Formation	64:87	Arabidopsis XTH4 and XTH9 Contribute to Wood Cell Expansion and Secondary Wall Formation.					
32005783	1	22	theme	walls	156:160	arg1	hemicellulose					114:126	the major hemicellulose	104:126	the major hemicellulose of dicotyledon primary cell walls	104:160	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	1	22	theme	walls	156:160	arg1	Xyloglucan					90:99	Xyloglucan	90:99	Xyloglucan	90:99	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	10	23	from	effects	1795:1801	arg1	xylem					1833:1837	secondary xylem	1823:1837	secondary xylem	1823:1837	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	6	24	theme	layers	1017:1022	arg1	number					987:992	the number	983:992	the number of secondary cell wall layers compared with the wild type	983:1050	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	5	25	theme	matrix	763:768	arg1	composition					776:786	matrix sugar composition	763:786	matrix sugar composition	763:786	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	6	26	theme	cell	1007:1010	arg1	layers					1017:1022	secondary cell wall layers	997:1022	secondary cell wall layers compared with the wild type	997:1050	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	2	27	theme	secondary	407:415	arg1	growth					417:422	secondary growth	407:422	secondary growth of Arabidopsis (Arabidopsis thaliana)	407:460	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	7	28	theme	specific	1202:1209	arg1	signals					1217:1223	specific Raman signals	1202:1223	specific Raman signals from the walls	1202:1238	These changes correlated with specific Raman signals from the walls, indicating changes in lignin and cellulose.					
32005783	5	29	from	walls	847:851	arg1	mutants					856:862	mutants	856:862	mutants	856:862	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	9	30	from	deficiency	1624:1633	arg1	XTH9					1647:1650	XTH9	1647:1650	XTH9	1647:1650	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	9	30	from	deficiency	1624:1633	arg1	XTH4					1638:1641	XTH4	1638:1641	XTH4	1638:1641	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	0	31	theme	Arabidopsis	0:10	arg1	XTH4					12:15	Arabidopsis XTH4	0:15	Arabidopsis XTH4	0:15	Arabidopsis XTH4 and XTH9 Contribute to Wood Cell Expansion and Secondary Wall Formation.					
32005783	2	32	theme	thaliana	452:459	arg1	growth					417:422	secondary growth	407:422	secondary growth of Arabidopsis (Arabidopsis thaliana)	407:460	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	3	33	theme	thickened	478:486	arg1	hypocotyls					488:497	secondarily thickened hypocotyls	466:497	secondarily thickened hypocotyls	466:497	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	3	34	theme	element	545:551	arg1	expansion					553:561	vessel element expansion	538:561	vessel element expansion	538:561	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	1	35	theme	dicotyledon	131:141	arg1	walls					156:160	dicotyledon primary cell walls	131:160	dicotyledon primary cell walls	131:160	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	5	36	theme	secondary	822:830	arg1	walls					847:851	secondary versus primary walls	822:851	secondary versus primary walls in mutants	822:862	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	10	37	dep	both	1910:1913	arg1	directly					1915:1922	directly	1915:1922	directly	1915:1922	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	5	38	theme	primary	839:845	arg1	walls					847:851	secondary versus primary walls	822:851	secondary versus primary walls in mutants	822:862	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	10	39	theme	wood	1894:1897	arg1	properties					1899:1908	wood properties	1894:1908	wood properties	1894:1908	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	5	40	from	changes	729:735	arg1	composition					776:786	matrix sugar composition	763:786	matrix sugar composition	763:786	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	5	40	from	changes	729:735	arg1	cellulose					748:756	cellulose	748:756	cellulose	748:756	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	5	40	from	changes	729:735	arg1	lignin					740:745	lignin	740:745	lignin	740:745	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	6	41	theme	mutant	1114:1119	arg1	xth4x9					1121:1126	the double mutant xth4x9	1103:1126	the double mutant xth4x9	1103:1126	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	1	42	theme	endo-transglycosylase/hydrolases	237:268	arg1	participation					209:221	the participation	205:221	the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs)	205:275	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	2	43	used	used	281:284	arg2	We					278:279	We	278:279	We	278:279	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	5	44	theme	overall	802:808	arg1	increase					810:817	an overall increase	799:817	an overall increase	799:817	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	5	45	theme	wall	683:686	arg1	analyses					688:695	Cell wall analyses	678:695	Cell wall analyses of inflorescence stems	678:718	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	3	46	theme	positive	518:525	arg1	effects					527:533	positive effects	518:533	positive effects	518:533	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	2	47	theme	gain-of	296:302	arg1	function					304:311	gain-of function	296:311	gain-of function	296:311	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	9	48	theme	wall	1666:1669	arg1	integrity					1671:1679	cell wall integrity	1661:1679	cell wall integrity signaling	1661:1689	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	4	49	theme	secondary	612:620	arg1	thickening					627:636	secondary wall thickening	612:636	secondary wall thickening	612:636	They also stimulated secondary wall thickening but reduced secondary xylem production.					
32005783	10	50	theme	integrity	1942:1950	arg1	sensing					1952:1958	cell wall integrity sensing	1932:1958	cell wall integrity sensing	1932:1958	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	2	51	theme	loss-	286:290	arg1	approaches					313:322	loss- and gain-of function approaches	286:322	loss- and gain-of function approaches	286:322	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	5	52	theme	production	892:901	arg1	indicative					865:874	indicative	865:874	indicative	865:874	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	0	53	theme	Wood	40:43	arg1	Expansion					50:58	Wood Cell Expansion	40:58	Wood Cell Expansion	40:58	Arabidopsis XTH4 and XTH9 Contribute to Wood Cell Expansion and Secondary Wall Formation.					
32005783	10	54	theme	cell	1932:1935	arg1	integrity					1942:1950	cell wall integrity	1932:1950	cell wall integrity sensing	1932:1958	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	6	55	theme	secondary	997:1005	arg1	layers					1017:1022	secondary cell wall layers	997:1022	secondary cell wall layers compared with the wild type	997:1050	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	3	56	theme	intrusive	573:581	arg1	growth					583:588	fiber intrusive growth	567:588	fiber intrusive growth	567:588	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	9	57	theme	wall	1473:1476	arg1	biosynthesis					1478:1489	secondary wall biosynthesis	1463:1489	secondary wall biosynthesis	1463:1489	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	6	58	theme	intermediate	1141:1152	arg1	number					1154:1159	an intermediate number	1138:1159	an intermediate number of layers	1138:1169	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	9	59	theme	WALL	1547:1550	arg1	KINASE2					1563:1569	WALL ASSOCIATED KINASE2	1547:1569	WALL ASSOCIATED KINASE2	1547:1569	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	4	60	theme	xylem	660:664	arg1	production					666:675	secondary xylem production	650:675	secondary xylem production	650:675	They also stimulated secondary wall thickening but reduced secondary xylem production.					
32005783	10	61	theme	XTH9	1815:1818	arg1	effects					1795:1801	Prominent effects	1785:1801	Prominent effects of XTH4 and XTH9 on secondary xylem	1785:1837	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	1	62	theme	load-bearing	177:188	arg1	framework					190:198	the load-bearing framework	173:198	the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs)	173:275	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	5	63	theme	higher	879:884	arg1	production					892:901	higher xylem production	879:901	higher xylem production	879:901	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	5	64	theme	stems	714:718	arg1	analyses					688:695	Cell wall analyses	678:695	Cell wall analyses of inflorescence stems	678:718	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	10	65	theme	XTH4	1806:1809	arg1	effects					1795:1801	Prominent effects	1785:1801	Prominent effects of XTH4 and XTH9 on secondary xylem	1785:1837	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	9	66	theme	wall	1500:1503	arg1	integrity					1505:1513	cell wall integrity	1495:1513	cell wall integrity	1495:1513	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	9	67	theme	xylem	1722:1726	arg1	production					1733:1742	xylem cell production	1722:1742	xylem cell production	1722:1742	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	5	68	from	increase	810:817	arg1	walls					847:851	secondary versus primary walls	822:851	secondary versus primary walls in mutants	822:862	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	7	69	from	walls	1234:1238	arg1	signals					1217:1223	specific Raman signals	1202:1223	specific Raman signals from the walls	1202:1238	These changes correlated with specific Raman signals from the walls, indicating changes in lignin and cellulose.					
32005783	10	70	theme	secondary	1823:1831	arg1	xylem					1833:1837	secondary xylem	1823:1837	secondary xylem	1823:1837	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	5	71	theme	indicative	865:874	arg1	increase					810:817	an overall increase	799:817	an overall increase	799:817	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	5	72	theme	wild	921:924	arg1	type					926:929	the wild type	917:929	the wild type (since secondary walls were thinner)	917:966	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	0	73	theme	Wall	74:77	arg1	Formation					79:87	Secondary Wall Formation	64:87	Secondary Wall Formation	64:87	Arabidopsis XTH4 and XTH9 Contribute to Wood Cell Expansion and Secondary Wall Formation.					
32005783	1	74	theme	cell	151:154	arg1	walls					156:160	dicotyledon primary cell walls	131:160	dicotyledon primary cell walls	131:160	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	9	75	theme	wall	1768:1771	arg1	thickening					1773:1782	secondary wall thickening	1758:1782	secondary wall thickening	1758:1782	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	8	76	theme	interfascicular	1327:1341	arg1	fibers					1343:1348	the interfascicular fibers	1323:1348	the interfascicular fibers	1323:1348	Secondary walls were affected also in the interfascicular fibers, where neither XTH4 nor XTH9 was expressed, indicating that these effects were indirect.					
32005783	7	77	theme	Raman	1211:1215	arg1	signals					1217:1223	specific Raman signals	1202:1223	specific Raman signals from the walls	1202:1238	These changes correlated with specific Raman signals from the walls, indicating changes in lignin and cellulose.					
32005783	9	78	theme	ASSOCIATED	1552:1561	arg1	KINASE2					1563:1569	WALL ASSOCIATED KINASE2	1547:1569	WALL ASSOCIATED KINASE2	1547:1569	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	6	79	theme	wall	1012:1015	arg1	layers					1017:1022	secondary cell wall layers	997:1022	secondary cell wall layers compared with the wild type	997:1050	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	5	80	theme	sugar	770:774	arg1	composition					776:786	matrix sugar composition	763:786	matrix sugar composition	763:786	Cell wall analyses of inflorescence stems revealed changes in lignin, cellulose, and matrix sugar composition indicating an overall increase in secondary versus primary walls in mutants, indicative of higher xylem production compared with the wild type (since secondary walls were thinner).					
32005783	3	81	contain	had	514:516	arg1	enzymes					506:512	these enzymes	500:512	these enzymes	500:512	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	3	81	contain	had	514:516	arg2	effects					527:533	positive effects	518:533	positive effects	518:533	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
32005783	2	82	theme	cambial	384:390	arg1	regions					392:398	cambial regions	384:398	cambial regions	384:398	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	2	83	theme	Arabidopsis	440:450	arg1	thaliana					452:459	Arabidopsis (Arabidopsis thaliana)	427:460	Arabidopsis (Arabidopsis thaliana)	427:460	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	10	84	theme	altered	1867:1873	arg1	xyloglucan					1875:1884	altered xyloglucan	1867:1884	altered xyloglucan	1867:1884	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	2	85	theme	Arabidopsis	427:437	arg1	thaliana					452:459	Arabidopsis (Arabidopsis thaliana)	427:460	Arabidopsis (Arabidopsis thaliana)	427:460	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	1	86	with	framework	190:198	arg1	participation					209:221	the participation	205:221	the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs)	205:275	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	0	87	theme	Cell	45:48	arg1	Expansion					50:58	Wood Cell Expansion	40:58	Wood Cell Expansion	40:58	Arabidopsis XTH4 and XTH9 Contribute to Wood Cell Expansion and Secondary Wall Formation.					
32005783	2	88	theme	XTH4	346:349	arg1	functions					333:341	functions	333:341	functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana)	333:460	We used loss- and gain-of function approaches to study functions of XTH4 and XTH9 abundantly expressed in cambial regions during secondary growth of Arabidopsis (Arabidopsis thaliana).					
32005783	10	89	dep	affects	1886:1892	arg1	both					1910:1913	both	1910:1913	both	1910:1913	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	10	89	dep	affects	1886:1892	arg1	sensing					1952:1958	cell wall integrity sensing	1932:1958	cell wall integrity sensing	1932:1958	Prominent effects of XTH4 and XTH9 on secondary xylem support the hypothesis that altered xyloglucan affects wood properties both directly and via cell wall integrity sensing.					
32005783	1	90	theme	xyloglucan	226:235	arg1	endo-transglycosylase/hydrolases					237:268	xyloglucan endo-transglycosylase/hydrolases	226:268	xyloglucan endo-transglycosylase/hydrolases (XTHs)	226:275	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	1	90	theme	xyloglucan	226:235	arg1	XTHs					271:274	XTHs	271:274	XTHs	271:274	Xyloglucan is the major hemicellulose of dicotyledon primary cell walls, affecting the load-bearing framework with the participation of xyloglucan endo-transglycosylase/hydrolases (XTHs).					
32005783	9	91	theme	cell	1495:1498	arg1	integrity					1505:1513	cell wall integrity	1495:1513	cell wall integrity	1495:1513	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	6	92	theme	double	1107:1112	arg1	xth4x9					1121:1126	the double mutant xth4x9	1103:1126	the double mutant xth4x9	1103:1126	Intriguingly, the number of secondary cell wall layers compared with the wild type was increased in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of layers.					
32005783	4	93	theme	secondary	650:658	arg1	production					666:675	secondary xylem production	650:675	secondary xylem production	650:675	They also stimulated secondary wall thickening but reduced secondary xylem production.					
32005783	9	94	dep	biosynthesis	1478:1489	arg1	sensing					1515:1521	sensing	1515:1521	sensing	1515:1521	Transcripts involved in secondary wall biosynthesis and cell wall integrity sensing, including THESEUS1 and WALL ASSOCIATED KINASE2, were highly induced in the mutants, indicating that deficiency in XTH4 and XTH9 triggers cell wall integrity signaling, which, we propose, stimulates xylem cell production and modulates secondary wall thickening.					
32005783	3	95	theme	vessel	538:543	arg1	expansion					553:561	vessel element expansion	538:561	vessel element expansion	538:561	In secondarily thickened hypocotyls, these enzymes had positive effects on vessel element expansion and fiber intrusive growth.					
34530036	6	0	theme	millimeter	1174:1183	arg1	microspheres					1192:1203	millimeter hollow microspheres	1174:1203	millimeter hollow microspheres	1174:1203	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	1	theme	accessible	1095:1104	arg1	structure					1150:1158	its unique hollow structure	1132:1158	its unique hollow structure	1132:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	1	theme	accessible	1095:1104	arg1	amount					1106:1111	the large accessible amount	1085:1111	the large accessible amount of -NH2 groups and its unique hollow structure	1085:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	1	theme	accessible	1095:1104	arg1	groups					1121:1126	-NH2 groups	1116:1126	-NH2 groups	1116:1126	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	0	2	theme	dye	82:84	arg1	adsorption					86:95	dye adsorption	82:95	dye adsorption	82:95	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption.					
34530036	6	3	theme	hollow	1185:1190	arg1	microspheres					1192:1203	millimeter hollow microspheres	1174:1203	millimeter hollow microspheres	1174:1203	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	5	4	theme	excellent	985:993	arg1	performance					1006:1016	excellent adsorption performance	985:1016	excellent adsorption performance	985:1016	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	4	5	theme	hollow	857:862	arg1	microspheres					864:875	different hollow microspheres	847:875	different hollow microspheres	847:875	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	6	6	theme	large	1089:1093	arg1	structure					1150:1158	its unique hollow structure	1132:1158	its unique hollow structure	1132:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	6	theme	large	1089:1093	arg1	amount					1106:1111	the large accessible amount	1085:1111	the large accessible amount of -NH2 groups and its unique hollow structure	1085:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	6	theme	large	1089:1093	arg1	groups					1121:1126	-NH2 groups	1116:1126	-NH2 groups	1116:1126	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	4	7	theme	microspheres	864:875	arg1	formation					834:842	the formation	830:842	the formation of different hollow microspheres with varied shell thickness and size	830:912	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	6	8	theme	emerging	1258:1265	arg1	contaminants					1267:1278	emerging contaminants	1258:1278	emerging contaminants in wastewater	1258:1292	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	2	9	theme	treatment	366:374	arg1	time					376:379	different polyethyleneimine treatment time	338:379	different polyethyleneimine treatment time	338:379	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	5	10	theme	testing	1052:1058	arg1	conditions					1060:1069	testing conditions	1052:1069	testing conditions	1052:1069	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	3	11	dep	carboxyl	558:565	arg1	the					554:556	the	554:556	the	554:556	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	2	12	theme	polyethyleneimine	348:364	arg1	time					376:379	different polyethyleneimine treatment time	338:379	different polyethyleneimine treatment time	338:379	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	6	13	from	contaminants	1267:1278	arg1	wastewater					1283:1292	wastewater	1283:1292	wastewater	1283:1292	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	5	14	theme	targeted	1025:1032	arg1	methyl					1034:1039	targeted methyl blue	1025:1044	targeted methyl blue under testing conditions	1025:1069	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	5	15	theme	adsorption	995:1004	arg1	performance					1006:1016	excellent adsorption performance	985:1016	excellent adsorption performance	985:1016	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	6	16	theme	unique	1136:1141	arg1	structure					1150:1158	its unique hollow structure	1132:1158	its unique hollow structure	1132:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	2	17	theme	different	338:346	arg1	time					376:379	different polyethyleneimine treatment time	338:379	different polyethyleneimine treatment time	338:379	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	4	18	theme	polyethyleneimine	718:734	arg1	usage					690:694	usage	690:694	usage	690:694	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	4	18	theme	polyethyleneimine	718:734	arg1	time					710:713	treatment time	700:713	treatment time	700:713	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	6	19	theme	broad	1210:1214	arg1	prospects					1228:1236	broad application prospects	1210:1236	broad application prospects	1210:1236	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	3	20	theme	cellulose	597:605	arg1	microspheres					607:618	carboxymethyl cellulose microspheres	583:618	carboxymethyl cellulose microspheres	583:618	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	6	21	from	wastewater	1283:1292	arg1	treatment					1245:1253	the treatment	1241:1253	the treatment of emerging contaminants in wastewater	1241:1292	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	2	22	theme	area	460:463	arg1	analysis					465:472	BET surface area analysis	448:472	BET surface area analysis	448:472	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	3	23	from	carboxyl	558:565	arg1	microspheres					607:618	carboxymethyl cellulose microspheres	583:618	carboxymethyl cellulose microspheres	583:618	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	6	24	dep	amount	1106:1111	arg1	virtue					1075:1080	virtue	1075:1080	virtue	1075:1080	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	2	25	theme	surface	452:458	arg1	analysis					465:472	BET surface area analysis	448:472	BET surface area analysis	448:472	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	0	26	theme	Facile	0:5	arg1	transformation					7:20	Facile transformation	0:20	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption	0:95	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption.					
34530036	1	27	theme	Novel	98:102	arg1	microspheres					122:133	Novel millimeter hollow microspheres	98:133	Novel millimeter hollow microspheres	98:133	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34530036	2	28	theme	BET	448:450	arg1	analysis					465:472	BET surface area analysis	448:472	BET surface area analysis	448:472	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	0	29	theme	carboxymethyl	25:37	arg1	beads					49:53	carboxymethyl cellulose beads	25:53	carboxymethyl cellulose beads	25:53	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption.					
34530036	4	30	theme	shell	889:893	arg1	thickness					895:903	varied shell thickness	882:903	varied shell thickness	882:903	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	4	31	dep	usage	690:694	arg1	the					686:688	the	686:688	the	686:688	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	1	32	theme	millimeter	104:113	arg1	microspheres					122:133	Novel millimeter hollow microspheres	98:133	Novel millimeter hollow microspheres	98:133	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34530036	6	33	theme	hollow	1143:1148	arg1	structure					1150:1158	its unique hollow structure	1132:1158	its unique hollow structure	1132:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	5	34	theme	blue	1041:1044	arg1	methyl					1034:1039	targeted methyl blue	1025:1044	targeted methyl blue under testing conditions	1025:1069	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	3	35	theme	carboxymethyl	583:595	arg1	microspheres					607:618	carboxymethyl cellulose microspheres	583:618	carboxymethyl cellulose microspheres	583:618	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	6	36	contain	have	1205:1208	arg2	prospects					1228:1236	broad application prospects	1210:1236	broad application prospects	1210:1236	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	36	contain	have	1205:1208	arg1	type					1166:1169	this type	1161:1169	this type of millimeter hollow microspheres	1161:1203	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	1	37	theme	hollow	115:120	arg1	microspheres					122:133	Novel millimeter hollow microspheres	98:133	Novel millimeter hollow microspheres	98:133	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34530036	6	38	from	treatment	1245:1253	arg1	wastewater					1283:1292	wastewater	1283:1292	wastewater	1283:1292	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	1	39	theme	crosslinking	241:252	arg1	glutaraldehyde					221:234	glutaraldehyde	221:234	glutaraldehyde	221:234	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34530036	1	39	theme	crosslinking	241:252	arg1	agent					254:258	a crosslinking agent	239:258	a crosslinking agent	239:258	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34530036	0	40	theme	beads	49:53	arg1	transformation					7:20	Facile transformation	0:20	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption	0:95	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption.					
34530036	4	41	with	formation	834:842	arg1	size					909:912	size	909:912	size	909:912	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	4	41	with	formation	834:842	arg1	thickness					895:903	varied shell thickness	882:903	varied shell thickness	882:903	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	3	42	theme	structures	656:665	arg1	formation					636:644	the formation	632:644	the formation of hollow structures	632:665	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	5	43	theme	prepared	951:958	arg1	microspheres					967:978	the prepared hollow microspheres	947:978	the prepared hollow microspheres	947:978	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	0	44	theme	cellulose	39:47	arg1	beads					49:53	carboxymethyl cellulose beads	25:53	carboxymethyl cellulose beads	25:53	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption.					
34530036	3	45	theme	coordination	527:538	arg1	bonds					540:544	the coordination bonds	523:544	the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres	523:618	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	0	46	theme	hollow	60:65	arg1	composites					67:76	hollow composites	60:76	hollow composites for dye adsorption	60:95	Facile transformation of carboxymethyl cellulose beads into hollow composites for dye adsorption.					
34530036	5	47	theme	hollow	960:965	arg1	microspheres					967:978	the prepared hollow microspheres	947:978	the prepared hollow microspheres	947:978	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	2	48	theme	hollow	265:270	arg1	microspheres					272:283	The hollow microspheres	261:283	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time	261:379	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	6	49	theme	microspheres	1192:1203	arg1	type					1166:1169	this type	1161:1169	this type of millimeter hollow microspheres	1161:1203	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	4	50	theme	carboxymethyl	779:791	arg1	microspheres					803:814	the carboxymethyl cellulose microspheres	775:814	the carboxymethyl cellulose microspheres	775:814	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	6	51	theme	structure	1150:1158	arg1	structure					1150:1158	its unique hollow structure	1132:1158	its unique hollow structure	1132:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	51	theme	structure	1150:1158	arg1	amount					1106:1111	the large accessible amount	1085:1111	the large accessible amount of -NH2 groups and its unique hollow structure	1085:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	51	theme	structure	1150:1158	arg1	groups					1121:1126	-NH2 groups	1116:1126	-NH2 groups	1116:1126	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	3	52	theme	hollow	649:654	arg1	structures					656:665	hollow structures	649:665	hollow structures	649:665	It was shown that polyethyleneimine could break the coordination bonds between the carboxyl and Al (III) in carboxymethyl cellulose microspheres, leading to the formation of hollow structures.					
34530036	6	53	theme	application	1216:1226	arg1	prospects					1228:1236	broad application prospects	1210:1236	broad application prospects	1210:1236	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	4	54	theme	microspheres	803:814	arg1	structure					762:770	the structure	758:770	the structure of the carboxymethyl cellulose microspheres	758:814	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	6	55	theme	groups	1121:1126	arg1	structure					1150:1158	its unique hollow structure	1132:1158	its unique hollow structure	1132:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	55	theme	groups	1121:1126	arg1	amount					1106:1111	the large accessible amount	1085:1111	the large accessible amount of -NH2 groups and its unique hollow structure	1085:1158	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	6	55	theme	groups	1121:1126	arg1	groups					1121:1126	-NH2 groups	1116:1126	-NH2 groups	1116:1126	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	4	56	theme	different	847:855	arg1	microspheres					864:875	different hollow microspheres	847:875	different hollow microspheres	847:875	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	2	57	theme	polyethyleneimine	309:325	arg1	usages					327:332	different polyethyleneimine usages	299:332	different polyethyleneimine usages	299:332	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	4	58	theme	cellulose	793:801	arg1	microspheres					803:814	the carboxymethyl cellulose microspheres	775:814	the carboxymethyl cellulose microspheres	775:814	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	6	59	theme	-NH2	1116:1119	arg1	groups					1121:1126	-NH2 groups	1116:1126	-NH2 groups	1116:1126	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	4	60	theme	treatment	700:708	arg1	time					710:713	treatment time	700:713	treatment time	700:713	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	2	61	theme	different	299:307	arg1	usages					327:332	different polyethyleneimine usages	299:332	different polyethyleneimine usages	299:332	The hollow microspheres prepared with different polyethyleneimine usages and different polyethyleneimine treatment time were investigated deeply and characterized via SEM-EDX, FT-IR, and BET surface area analysis.					
34530036	5	62	contain	have	980:983	arg2	performance					1006:1016	excellent adsorption performance	985:1016	excellent adsorption performance	985:1016	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	5	62	contain	have	980:983	arg1	microspheres					967:978	the prepared hollow microspheres	947:978	the prepared hollow microspheres	947:978	Most importantly, we found that the prepared hollow microspheres have excellent adsorption performance toward targeted methyl blue under testing conditions.					
34530036	1	63	theme	carboxymethyl	156:168	arg1	microspheres					180:191	carboxymethyl cellulose microspheres	156:191	carboxymethyl cellulose microspheres	156:191	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34530036	6	64	theme	contaminants	1267:1278	arg1	treatment					1245:1253	the treatment	1241:1253	the treatment of emerging contaminants in wastewater	1241:1292	By virtue of the large accessible amount of -NH2 groups and its unique hollow structure, this type of millimeter hollow microspheres have broad application prospects in the treatment of emerging contaminants in wastewater.					
34530036	4	65	theme	varied	882:887	arg1	thickness					895:903	varied shell thickness	882:903	varied shell thickness	882:903	Most importantly, the usage and treatment time of polyethyleneimine can distinctly tailor the structure of the carboxymethyl cellulose microspheres, resulting in the formation of different hollow microspheres with varied shell thickness and size.					
34530036	1	66	theme	cellulose	170:178	arg1	microspheres					180:191	carboxymethyl cellulose microspheres	156:191	carboxymethyl cellulose microspheres	156:191	Novel millimeter hollow microspheres were fabricated from carboxymethyl cellulose microspheres and polyethyleneimine using glutaraldehyde as a crosslinking agent.					
34555749	7	0	theme	complete	1121:1128	arg1	utilization					1130:1140	complete utilization	1121:1140	complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass	1121:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	1	1	theme	biofuel	200:206	arg1	production					208:217	biofuel production	200:217	biofuel production	200:217	Biorefinery of biomass blends can achieve sustainable development of biofuel production.					
34555749	6	2	with	%	1039:1039	arg1	ratio					1063:1067	a mass ratio	1056:1067	a mass ratio of 2:1	1056:1074	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	4	3	theme	stable	789:794	arg1	products					796:803	stable products	789:803	stable products	789:803	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	4	4	theme	hemicellulose	762:774	arg1	autohydrolysis					738:751	the acidic autohydrolysis	727:751	the acidic autohydrolysis of CC/SB hemicellulose	727:774	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	3	5	dep	decomposition	544:556	arg1	90.59 kJ/mol					564:575	90.59 kJ/mol	564:575	90.59 kJ/mol	564:575	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB were lower than those for CC (126.12 kJ/mol) and CC/SB/BP (153.62 kJ/mol).					
34555749	3	5	dep	decomposition	544:556	arg1	Ea1					559:561	Ea1	559:561	Ea1	559:561	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB were lower than those for CC (126.12 kJ/mol) and CC/SB/BP (153.62 kJ/mol).					
34555749	4	6	dep	loading	710:716	arg1	having					667:672	having	667:672	having a high content of nitrogen sources	667:707	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	2	7	theme	sugarcane	331:339	arg1	SB					350:351	SB	350:351	SB	350:351	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	7	theme	sugarcane	331:339	arg1	bagasse					341:347	sugarcane bagasse	331:347	sugarcane bagasse (SB)	331:352	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	8	from	differences	274:284	arg1	SB					350:351	SB	350:351	SB	350:351	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	8	from	differences	274:284	arg1	BP					384:385	BP	384:385	BP	384:385	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	8	from	differences	274:284	arg1	bagasse					341:347	sugarcane bagasse	331:347	sugarcane bagasse (SB)	331:352	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	8	from	differences	274:284	arg1	papyrifera					372:381	Broussonetia papyrifera	359:381	Broussonetia papyrifera (BP)	359:386	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	8	from	differences	274:284	arg1	corncob					317:323	chemical composition-namely corncob	289:323	chemical composition-namely corncob (CC)	289:328	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	8	from	differences	274:284	arg1	CC					326:327	CC	326:327	CC	326:327	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	6	9	theme	xylose	1017:1022	arg1	yield					1024:1028	The highest total xylose yield	999:1028	The highest total xylose yield	999:1028	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	6	9	theme	xylose	1017:1022	arg1	%					1039:1039	83.64%	1034:1039	83.64% for CC/SB	1034:1049	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	4	10	theme	acidic	731:736	arg1	autohydrolysis					738:751	the acidic autohydrolysis	727:751	the acidic autohydrolysis of CC/SB hemicellulose	727:774	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	1	11	theme	production	208:217	arg1	development					185:195	sustainable development	173:195	sustainable development of biofuel production	173:217	Biorefinery of biomass blends can achieve sustainable development of biofuel production.					
34555749	7	12	theme	ethanol	1197:1203	arg1	co-production					1180:1192	co-production	1180:1192	co-production of ethanol and microalga biomass	1180:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	7	13	theme	pentose	1153:1159	arg1	utilization					1130:1140	complete utilization	1121:1140	complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass	1121:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	0	14	theme	Hydrothermal	0:11	arg1	thermodynamics					98:111	thermodynamics	98:111	thermodynamics	98:111	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	14	theme	Hydrothermal	0:11	arg1	Kinetics					88:95	Kinetics	88:95	Kinetics	88:95	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	14	theme	Hydrothermal	0:11	arg1	co-hydrolysis					13:25	Hydrothermal co-hydrolysis	0:25	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.	0:129	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	14	theme	Hydrothermal	0:11	arg1	fermentation					117:128	fermentation	117:128	fermentation	117:128	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	4	15	theme	high	676:679	arg1	content					681:687	a high content	674:687	a high content of nitrogen sources	674:707	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	2	16	theme	blends	499:504	arg1	kinetics					450:457	their hydrothermal co-hydrolysis kinetics	417:457	their hydrothermal co-hydrolysis kinetics	417:457	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	16	theme	blends	499:504	arg1	thermodynamics					463:476	thermodynamics	463:476	thermodynamics	463:476	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	7	17	theme	biomass	1087:1093	arg1	blend					1095:1099	biomass blend	1087:1099	biomass blend of CC/SB/BP	1087:1111	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	3	18	theme	CC	614:615	arg1	126.12 kJ/mol					618:630	CC (126.12 kJ/mol)	614:631	CC (126.12 kJ/mol)	614:631	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB were lower than those for CC (126.12 kJ/mol) and CC/SB/BP (153.62 kJ/mol).					
34555749	4	19	theme	nitrogen	692:699	arg1	sources					701:707	nitrogen sources	692:707	nitrogen sources	692:707	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	2	20	theme	biomass	491:497	arg1	blends					499:504	different biomass blends	481:504	different biomass blends	481:504	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	6	21	theme	total	1011:1015	arg1	yield					1024:1028	The highest total xylose yield	999:1028	The highest total xylose yield	999:1028	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	6	21	theme	total	1011:1015	arg1	%					1039:1039	83.64%	1034:1039	83.64% for CC/SB	1034:1049	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	4	22	theme	CC/SB	756:760	arg1	hemicellulose					762:774	CC/SB hemicellulose	756:774	CC/SB hemicellulose	756:774	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	5	23	theme	different	919:927	arg1	biomass					929:935	different biomass	919:935	different biomass	919:935	Cumulative feedback inhibition occurred among different biomass, and it could be minimized by controlling the blending ratio.					
34555749	2	24	theme	different	481:489	arg1	blends					499:504	different biomass blends	481:504	different biomass blends	481:504	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	4	25	theme	negative	825:832	arg1	value					842:846	the negative entropy value	821:846	the negative entropy value for CC/SB/BP hydrolysis	821:870	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	5	26	theme	blending	983:990	arg1	ratio					992:996	the blending ratio	979:996	the blending ratio	979:996	Cumulative feedback inhibition occurred among different biomass, and it could be minimized by controlling the blending ratio.					
34555749	7	27	theme	CC/SB/BP	1104:1111	arg1	blend					1095:1099	biomass blend	1087:1099	biomass blend of CC/SB/BP	1087:1111	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	7	28	theme	acids	1171:1175	arg1	utilization					1130:1140	complete utilization	1121:1140	complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass	1121:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	2	29	with	wastes	250:255	arg1	differences					274:284	significant differences	262:284	significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)	262:386	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	4	30	theme	BP	663:664	arg1	loading					710:716	BP (having a high content of nitrogen sources) loading	663:716	BP (having a high content of nitrogen sources) loading	663:716	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	0	31	theme	papyrifera	69:78	arg1	blends					80:85	corncob/sugarcane bagasse/Broussonetia papyrifera blends	30:85	corncob/sugarcane bagasse/Broussonetia papyrifera blends	30:85	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	6	32	theme	mass	1058:1061	arg1	ratio					1063:1067	a mass ratio	1056:1067	a mass ratio of 2:1	1056:1074	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	2	33	theme	chemical	289:296	arg1	corncob					317:323	chemical composition-namely corncob	289:323	chemical composition-namely corncob (CC)	289:328	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	33	theme	chemical	289:296	arg1	CC					326:327	CC	326:327	CC	326:327	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	6	34	theme	2:1	1072:1074	arg1	ratio					1063:1067	a mass ratio	1056:1067	a mass ratio of 2:1	1056:1074	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	5	35	theme	Cumulative	873:882	arg1	inhibition					893:902	Cumulative feedback inhibition	873:902	Cumulative feedback inhibition	873:902	Cumulative feedback inhibition occurred among different biomass, and it could be minimized by controlling the blending ratio.					
34555749	2	36	theme	hydrothermal	423:434	arg1	kinetics					450:457	their hydrothermal co-hydrolysis kinetics	417:457	their hydrothermal co-hydrolysis kinetics	417:457	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	0	37	theme	bagasse/Broussonetia	48:67	arg1	blends					80:85	corncob/sugarcane bagasse/Broussonetia papyrifera blends	30:85	corncob/sugarcane bagasse/Broussonetia papyrifera blends	30:85	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	38	theme	corncob/sugarcane	30:46	arg1	blends					80:85	corncob/sugarcane bagasse/Broussonetia papyrifera blends	30:85	corncob/sugarcane bagasse/Broussonetia papyrifera blends	30:85	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	5	39	theme	feedback	884:891	arg1	inhibition					893:902	Cumulative feedback inhibition	873:902	Cumulative feedback inhibition	873:902	Cumulative feedback inhibition occurred among different biomass, and it could be minimized by controlling the blending ratio.					
34555749	2	40	theme	co-hydrolysis	436:448	arg1	kinetics					450:457	their hydrothermal co-hydrolysis kinetics	417:457	their hydrothermal co-hydrolysis kinetics	417:457	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	7	41	theme	hexose	1145:1150	arg1	utilization					1130:1140	complete utilization	1121:1140	complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass	1121:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	6	42	theme	highest	1003:1009	arg1	yield					1024:1028	The highest total xylose yield	999:1028	The highest total xylose yield	999:1028	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	6	42	theme	highest	1003:1009	arg1	%					1039:1039	83.64%	1034:1039	83.64% for CC/SB	1034:1049	The highest total xylose yield was 83.64% for CC/SB with a mass ratio of 2:1.					
34555749	4	43	theme	CC/SB/BP	852:859	arg1	hydrolysis					861:870	CC/SB/BP hydrolysis	852:870	CC/SB/BP hydrolysis	852:870	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	1	44	theme	biomass	146:152	arg1	blends					154:159	biomass blends	146:159	biomass blends	146:159	Biorefinery of biomass blends can achieve sustainable development of biofuel production.					
34555749	0	45	theme	blends	80:85	arg1	thermodynamics					98:111	thermodynamics	98:111	thermodynamics	98:111	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	45	theme	blends	80:85	arg1	Kinetics					88:95	Kinetics	88:95	Kinetics	88:95	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	45	theme	blends	80:85	arg1	co-hydrolysis					13:25	Hydrothermal co-hydrolysis	0:25	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.	0:129	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	45	theme	blends	80:85	arg1	fermentation					117:128	fermentation	117:128	fermentation	117:128	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	7	46	dep	ethanol	1197:1203	arg1	biomass					1219:1225	biomass	1219:1225	biomass	1219:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	2	47	theme	significant	262:272	arg1	differences					274:284	significant differences	262:284	significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)	262:386	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	1	48	theme	blends	154:159	arg1	Biorefinery					131:141	Biorefinery	131:141	Biorefinery of biomass blends	131:159	Biorefinery of biomass blends can achieve sustainable development of biofuel production.					
34555749	7	49	theme	amino	1165:1169	arg1	acids					1171:1175	amino acids	1165:1175	amino acids	1165:1175	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34555749	4	50	theme	entropy	834:840	arg1	value					842:846	the negative entropy value	821:846	the negative entropy value for CC/SB/BP hydrolysis	821:870	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	2	51	theme	Broussonetia	359:370	arg1	papyrifera					372:381	Broussonetia papyrifera	359:381	Broussonetia papyrifera (BP)	359:386	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	51	theme	Broussonetia	359:370	arg1	BP					384:385	BP	384:385	BP	384:385	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	52	dep	-were	387:391	arg1	selected					393:400	selected	393:400	-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends	387:504	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	53	theme	lignocellulosic	234:248	arg1	wastes					250:255	three lignocellulosic wastes	228:255	three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)	228:386	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	3	54	theme	decomposition	544:556	arg1	energies					518:525	Activation energies	507:525	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB	507:586	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB were lower than those for CC (126.12 kJ/mol) and CC/SB/BP (153.62 kJ/mol).					
34555749	3	55	theme	hemicellulose	530:542	arg1	decomposition					544:556	hemicellulose decomposition	530:556	hemicellulose decomposition (Ea1, 90.59 kJ/mol)	530:576	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB were lower than those for CC (126.12 kJ/mol) and CC/SB/BP (153.62 kJ/mol).					
34555749	3	56	theme	Activation	507:516	arg1	energies					518:525	Activation energies	507:525	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB	507:586	Activation energies of hemicellulose decomposition (Ea1, 90.59 kJ/mol) for CC/SB were lower than those for CC (126.12 kJ/mol) and CC/SB/BP (153.62 kJ/mol).					
34555749	0	57	dep	co-hydrolysis	13:25	arg1	thermodynamics					98:111	thermodynamics	98:111	thermodynamics	98:111	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	57	dep	co-hydrolysis	13:25	arg1	Kinetics					88:95	Kinetics	88:95	Kinetics	88:95	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	57	dep	co-hydrolysis	13:25	arg1	co-hydrolysis					13:25	Hydrothermal co-hydrolysis	0:25	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.	0:129	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	0	57	dep	co-hydrolysis	13:25	arg1	fermentation					117:128	fermentation	117:128	fermentation	117:128	Hydrothermal co-hydrolysis of corncob/sugarcane bagasse/Broussonetia papyrifera blends: Kinetics, thermodynamics and fermentation.					
34555749	1	58	theme	sustainable	173:183	arg1	development					185:195	sustainable development	173:195	sustainable development of biofuel production	173:217	Biorefinery of biomass blends can achieve sustainable development of biofuel production.					
34555749	2	59	theme	composition-namely	298:315	arg1	corncob					317:323	chemical composition-namely corncob	289:323	chemical composition-namely corncob (CC)	289:328	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	2	59	theme	composition-namely	298:315	arg1	CC					326:327	CC	326:327	CC	326:327	Herein, three lignocellulosic wastes with significant differences in chemical composition-namely corncob (CC), sugarcane bagasse (SB), and Broussonetia papyrifera (BP)-were selected to investigate their hydrothermal co-hydrolysis kinetics and thermodynamics of different biomass blends.					
34555749	4	60	theme	sources	701:707	arg1	content					681:687	a high content	674:687	a high content of nitrogen sources	674:707	BP (having a high content of nitrogen sources) loading weakened the acidic autohydrolysis of CC/SB hemicellulose, but yielded stable products as indicated by the negative entropy value for CC/SB/BP hydrolysis.					
34555749	7	61	theme	microalga	1209:1217	arg1	co-production					1180:1192	co-production	1180:1192	co-production of ethanol and microalga biomass	1180:1225	Moreover, biomass blend of CC/SB/BP enabled complete utilization of hexose, pentose and amino acids by co-production of ethanol and microalga biomass.					
34064591	4	0	theme	glucose	714:720	arg1	characteristics					732:746	The glucose detection characteristics	710:746	The glucose detection characteristics of the fabricated biosensor	710:774	The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV).					
34064591	2	1	theme	TiO2	387:390	arg1	nanoparticles					392:404	the deposited TiO2 nanoparticles	373:404	the deposited TiO2 nanoparticles	373:404	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	1	2	from	nanoparticles	168:180	arg1	plasma					193:198	thermal plasma	185:198	thermal plasma	185:198	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	2	3	theme	deposited	377:385	arg1	nanoparticles					392:404	the deposited TiO2 nanoparticles	373:404	the deposited TiO2 nanoparticles	373:404	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	5	4	theme	μM	933:934	arg1	limit					920:924	low detection limit	906:924	low detection limit of 6.7 μM	906:934	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	4	theme	μM	933:934	arg1	0.9957					894:899	0.9957	894:899	0.9957	894:899	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	4	theme	μM	933:934	arg1	cm-2					883:886	302.0 µA mM-1 cm-2	869:886	302.0 µA mM-1 cm-2 (R2 = 0.9957)	869:900	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	3	5	theme	layer	566:570	arg1	composition					503:513	chemical composition	494:513	chemical composition	494:513	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	5	theme	layer	566:570	arg1	properties					530:539	structural properties	519:539	structural properties	519:539	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	6	theme	conductive	580:589	arg1	films					599:603	the conductive polymer films	576:603	the conductive polymer films	576:603	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	4	7	theme	biosensor	766:774	arg1	characteristics					732:746	The glucose detection characteristics	710:746	The glucose detection characteristics of the fabricated biosensor	710:774	The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV).					
34064591	6	8	theme	excellent	990:998	arg1	selectivity					1000:1010	excellent selectivity	990:1010	excellent selectivity	990:1010	The easily manufactured CS-PPy/TiO2 biosensor showed excellent selectivity and reactivity.					
34064591	3	9	theme	structural	519:528	arg1	properties					530:539	structural properties	519:539	structural properties	519:539	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	10	theme	chemical	494:501	arg1	composition					503:513	chemical composition	494:513	chemical composition	494:513	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	4	11	theme	fabricated	755:764	arg1	biosensor					766:774	the fabricated biosensor	751:774	the fabricated biosensor	751:774	The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV).					
34064591	3	12	theme	TiO2	548:551	arg1	layer					566:570	the TiO2 nanoparticle layer	544:570	the TiO2 nanoparticle layer	544:570	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	1	13	theme	thermal	185:191	arg1	plasma					193:198	thermal plasma	185:198	thermal plasma	185:198	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	5	14	theme	CS-PPy/TiO2	820:830	arg1	biosensor					832:840	CS-PPy/TiO2 biosensor	820:840	CS-PPy/TiO2 biosensor	820:840	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	15	theme	low	906:908	arg1	limit					920:924	low detection limit	906:924	low detection limit of 6.7 μM	906:934	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	3	16	theme	nanoparticle	553:564	arg1	layer					566:570	the TiO2 nanoparticle layer	544:570	the TiO2 nanoparticle layer	544:570	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	2	17	theme	X-ray	423:427	arg1	XRD					442:444	XRD	442:444	XRD	442:444	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	2	17	theme	X-ray	423:427	arg1	diffraction					429:439	X-ray diffraction	423:439	X-ray diffraction (XRD)	423:445	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	0	18	theme	Glucose	14:20	arg1	Biosensor					22:30	Non-Enzymatic Glucose Biosensor	0:30	Non-Enzymatic Glucose Biosensor	0:30	Non-Enzymatic Glucose Biosensor Based on Highly Pure TiO2 Nanoparticles.					
34064591	6	19	theme	manufactured	948:959	arg1	biosensor					973:981	The easily manufactured CS-PPy/TiO2 biosensor	937:981	The easily manufactured CS-PPy/TiO2 biosensor	937:981	The easily manufactured CS-PPy/TiO2 biosensor showed excellent selectivity and reactivity.					
34064591	3	20	theme	polymer	591:597	arg1	films					599:603	the conductive polymer films	576:603	the conductive polymer films	576:603	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	0	21	theme	Non-Enzymatic	0:12	arg1	Biosensor					22:30	Non-Enzymatic Glucose Biosensor	0:30	Non-Enzymatic Glucose Biosensor	0:30	Non-Enzymatic Glucose Biosensor Based on Highly Pure TiO2 Nanoparticles.					
34064591	1	22	theme	conductive	292:301	arg1	films					311:315	chitosan-polypyrrole (CS-PPy) conductive polymer films	262:315	chitosan-polypyrrole (CS-PPy) conductive polymer films	262:315	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	3	23	theme	films	599:603	arg1	composition					503:513	chemical composition	494:513	chemical composition	494:513	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	23	theme	films	599:603	arg1	properties					530:539	structural properties	519:539	structural properties	519:539	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	4	24	theme	cyclic	795:800	arg1	CV					815:816	CV	815:816	CV	815:816	The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV).					
34064591	4	24	theme	cyclic	795:800	arg1	voltammetry					802:812	cyclic voltammetry	795:812	cyclic voltammetry (CV)	795:817	The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV).					
34064591	2	25	theme	light	459:463	arg1	scattering					465:474	dynamic light scattering	451:474	dynamic light scattering (DLS)	451:480	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	2	25	theme	light	459:463	arg1	DLS					477:479	DLS	477:479	DLS	477:479	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	1	26	theme	polymer	303:309	arg1	films					311:315	chitosan-polypyrrole (CS-PPy) conductive polymer films	262:315	chitosan-polypyrrole (CS-PPy) conductive polymer films	262:315	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	1	27	theme	non-enzymatic	95:107	arg1	sensor					117:122	a non-enzymatic glucose sensor	93:122	a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method	93:341	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	2	28	theme	dynamic	451:457	arg1	scattering					465:474	dynamic light scattering	451:474	dynamic light scattering (DLS)	451:480	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	2	28	theme	dynamic	451:457	arg1	DLS					477:479	DLS	477:479	DLS	477:479	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	6	29	theme	CS-PPy/TiO2	961:971	arg1	biosensor					973:981	The easily manufactured CS-PPy/TiO2 biosensor	937:981	The easily manufactured CS-PPy/TiO2 biosensor	937:981	The easily manufactured CS-PPy/TiO2 biosensor showed excellent selectivity and reactivity.					
34064591	3	30	dep	composition	503:513	arg1	The					490:492	The	490:492	The	490:492	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	1	31	theme	glucose	109:115	arg1	sensor					117:122	a non-enzymatic glucose sensor	93:122	a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method	93:341	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	5	32	theme	high	849:852	arg1	sensitivity					854:864	high sensitivity	849:864	high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM	849:934	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	33	theme	limit	920:924	arg1	sensitivity					854:864	high sensitivity	849:864	high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM	849:934	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	3	34	theme	scanning	674:681	arg1	microscope					692:701	scanning electron microscope	674:701	scanning electron microscope (SEM)	674:707	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	34	theme	scanning	674:681	arg1	SEM					704:706	SEM	704:706	SEM	704:706	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	5	35	theme	µA	875:876	arg1	0.9957					894:899	0.9957	894:899	0.9957	894:899	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	35	theme	µA	875:876	arg1	cm-2					883:886	302.0 µA mM-1 cm-2	869:886	302.0 µA mM-1 cm-2 (R2 = 0.9957)	869:900	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	36	theme	cm-2	883:886	arg1	sensitivity					854:864	high sensitivity	849:864	high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM	849:934	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	1	37	theme	electrochemical	320:334	arg1	method					336:341	electrochemical method	320:341	electrochemical method	320:341	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	3	38	theme	X-ray	623:627	arg1	spectra					662:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	5	39	theme	mM-1	878:881	arg1	0.9957					894:899	0.9957	894:899	0.9957	894:899	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	5	39	theme	mM-1	878:881	arg1	cm-2					883:886	302.0 µA mM-1 cm-2	869:886	302.0 µA mM-1 cm-2 (R2 = 0.9957)	869:900	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	0	40	theme	TiO2	53:56	arg1	Nanoparticles					58:70	Highly Pure TiO2 Nanoparticles	41:70	Highly Pure TiO2 Nanoparticles	41:70	Non-Enzymatic Glucose Biosensor Based on Highly Pure TiO2 Nanoparticles.					
34064591	5	41	theme	detection	910:918	arg1	limit					920:924	low detection limit	906:924	low detection limit of 6.7 μM	906:934	CS-PPy/TiO2 biosensor showed high sensitivity of 302.0 µA mM-1 cm-2 (R2 = 0.9957) and low detection limit of 6.7 μM.					
34064591	0	42	theme	Pure	48:51	arg1	Nanoparticles					58:70	Highly Pure TiO2 Nanoparticles	41:70	Highly Pure TiO2 Nanoparticles	41:70	Non-Enzymatic Glucose Biosensor Based on Highly Pure TiO2 Nanoparticles.					
34064591	1	43	theme	TiO2	163:166	arg1	nanoparticles					168:180	high-purity TiO2 nanoparticles	151:180	high-purity TiO2 nanoparticles in thermal plasma	151:198	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	4	44	theme	detection	722:730	arg1	characteristics					732:746	The glucose detection characteristics	710:746	The glucose detection characteristics of the fabricated biosensor	710:774	The glucose detection characteristics of the fabricated biosensor were determined by cyclic voltammetry (CV).					
34064591	3	45	theme	photoelectron	629:641	arg1	spectra					662:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	46	theme	electron	683:690	arg1	microscope					692:701	scanning electron microscope	674:701	scanning electron microscope (SEM)	674:707	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	46	theme	electron	683:690	arg1	SEM					704:706	SEM	704:706	SEM	704:706	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	3	47	theme	XPS	657:659	arg1	spectra					662:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	2	48	theme	structural	348:357	arg1	properties					359:368	The structural properties	344:368	The structural properties of the deposited TiO2 nanoparticles	344:404	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	2	49	dep	diffraction	429:439	arg1	system					482:487	system	482:487	system	482:487	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34064591	1	50	theme	high-purity	151:161	arg1	nanoparticles					168:180	high-purity TiO2 nanoparticles	151:180	high-purity TiO2 nanoparticles in thermal plasma	151:198	This study proposes a non-enzymatic glucose sensor fabricated by synthesizing high-purity TiO2 nanoparticles in thermal plasma and depositing it directly on a substrate and then depositing chitosan-polypyrrole (CS-PPy) conductive polymer films by electrochemical method.					
34064591	3	51	theme	spectroscopy	643:654	arg1	spectra					662:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	X-ray photoelectron spectroscopy (XPS) spectra	623:668	The chemical composition and structural properties of the TiO2 nanoparticle layer and the conductive polymer films were confirmed by X-ray photoelectron spectroscopy (XPS) spectra and scanning electron microscope (SEM).					
34064591	2	52	theme	nanoparticles	392:404	arg1	properties					359:368	The structural properties	344:368	The structural properties of the deposited TiO2 nanoparticles	344:404	The structural properties of the deposited TiO2 nanoparticles were analyzed by X-ray diffraction (XRD) and dynamic light scattering (DLS) system.					
34785622	0	0	theme	engineering	76:86	arg1	approach					88:95	a bone tissue engineering approach	62:95	a bone tissue engineering approach	62:95	Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach.					
34785622	5	1	contain	have	766:769	arg2	stiffness					866:874	a stiffness	864:874	a stiffness ranging from 1 to 2 MPa	864:898	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	1	contain	have	766:769	arg2	porosities					771:780	porosities	771:780	porosities up to 86%	771:790	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	1	contain	have	766:769	arg2	sizes					827:831	pore sizes	822:831	pore sizes in the range of 60-170 μm	822:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	1	contain	have	766:769	arg2	interconnectivity					793:809	interconnectivity	793:809	interconnectivity up to 96%	793:819	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	1	contain	have	766:769	arg1	scaffolds					756:764	the obtained scaffolds	743:764	the obtained scaffolds	743:764	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	2	from	stiffness	866:874	arg1	range					840:844	the range	836:844	the range of 60-170 μm	836:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	3	from	porosities	771:780	arg1	range					840:844	the range	836:844	the range of 60-170 μm	836:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	0	4	theme	tissue	69:74	arg1	approach					88:95	a bone tissue engineering approach	62:95	a bone tissue engineering approach	62:95	Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach.					
34785622	3	5	theme	bone	527:530	arg1	solutions					545:553	bone regeneration solutions	527:553	bone regeneration solutions	527:553	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	4	6	theme	phosphate-based	614:628	arg1	scaffolds					630:638	chitosan/beta-tricalcium phosphate-based scaffolds	589:638	chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin	589:663	The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering.					
34785622	6	7	theme	culture	1081:1087	arg1	d					1076:1076	14 d	1073:1076	14 d of culture	1073:1087	Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture.					
34785622	5	8	theme	μm	856:857	arg1	range					840:844	the range	836:844	the range of 60-170 μm	836:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	2	9	theme	tissue	273:278	arg1	engineering					280:290	tissue engineering	273:290	tissue engineering	273:290	By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process.					
34785622	6	10	theme	cell	1037:1040	arg1	growth					1042:1047	cell growth	1037:1047	cell growth	1037:1047	Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture.					
34785622	3	11	theme	bioactive	417:425	arg1	agents					427:432	bioactive agents	417:432	bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions	417:553	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	3	12	theme	biological	453:462	arg1	performance					479:489	their biological and mechanical performance	447:489	their biological and mechanical performance	447:489	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	5	13	dep	%	819:819	arg1	96					817:818	96	817:818	96	817:818	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	14	dep	porosities	771:780	arg1	%					790:790	%	790:790	%	790:790	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	15	dep	2	894:894	arg1	to					891:892	to	891:892	to	891:892	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	1	16	theme	uneven	180:185	arg1	growth					198:203	uneven new tissue growth	180:203	uneven new tissue growth	180:203	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	0	17	theme	Chitosan/β-TCP	0:13	arg1	composites					15:24	Chitosan/β-TCP composites	0:24	Chitosan/β-TCP composites	0:24	Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach.					
34785622	6	18	theme	apatite	978:984	arg1	crystals					986:993	apatite crystals	978:993	apatite crystals	978:993	Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture.					
34785622	1	19	theme	new	187:189	arg1	growth					198:203	uneven new tissue growth	180:203	uneven new tissue growth	180:203	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	3	20	theme	Polymeric	370:378	arg1	scaffolds					380:388	Polymeric scaffolds	370:388	Polymeric scaffolds	370:388	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	1	21	theme	Bone	98:101	arg1	repair					128:133	natural repair	120:133	natural repair	120:133	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	21	theme	Bone	98:101	arg1	processes					153:161	long-standing processes	139:161	long-standing processes that can lead to uneven new tissue growth	139:203	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	21	theme	Bone	98:101	arg1	regeneration					103:114	Bone regeneration	98:114	Bone regeneration	98:114	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	22	theme	tissue	191:196	arg1	growth					198:203	uneven new tissue growth	180:203	uneven new tissue growth	180:203	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	4	23	theme	chitosan/beta-tricalcium	589:612	arg1	scaffolds					630:638	chitosan/beta-tricalcium phosphate-based scaffolds	589:638	chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin	589:663	The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering.					
34785622	5	24	dep	96	817:818	arg1	to					814:815	to	814:815	to	814:815	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	2	25	theme	new	301:303	arg1	approaches					305:314	new approaches	301:314	new approaches	301:314	By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process.					
34785622	1	26	theme	long-standing	139:151	arg1	repair					128:133	natural repair	120:133	natural repair	120:133	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	26	theme	long-standing	139:151	arg1	processes					153:161	long-standing processes	139:161	long-standing processes that can lead to uneven new tissue growth	139:203	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	26	theme	long-standing	139:151	arg1	regeneration					103:114	Bone regeneration	98:114	Bone regeneration	98:114	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	0	27	theme	silk	48:51	arg1	fibroin					53:59	silk fibroin	48:59	silk fibroin	48:59	Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach.					
34785622	8	28	theme	suitable	1237:1244	arg1	structures					1222:1231	the developed structures	1208:1231	the developed structures	1208:1231	These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.					
34785622	8	28	theme	suitable	1237:1244	arg1	candidates					1246:1255	suitable candidates	1237:1255	suitable candidates	1237:1255	These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.					
34785622	5	29	dep	interconnectivity	793:809	arg1	%					819:819	%	819:819	%	819:819	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	8	30	theme	tissue	1273:1278	arg1	engineering					1280:1290	bone tissue engineering	1268:1290	bone tissue engineering	1268:1290	These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.					
34785622	5	31	dep	86	788:789	arg1	to					785:786	to	785:786	to	785:786	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	4	32	theme	bone	698:701	arg1	engineering					710:720	bone tissue engineering	698:720	bone tissue engineering	698:720	The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering.					
34785622	3	33	theme	agents	427:432	arg1	incorporation					400:412	the incorporation	396:412	the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions	396:553	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	7	34	theme	cellular	1099:1106	arg1	proliferation					1108:1120	cellular proliferation	1099:1120	cellular proliferation	1099:1120	Besides, cellular proliferation was higher on the scaffolds coated with silk.					
34785622	3	35	theme	mechanical	468:477	arg1	performance					479:489	their biological and mechanical performance	447:489	their biological and mechanical performance	447:489	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	5	36	theme	pore	822:825	arg1	sizes					827:831	pore sizes	822:831	pore sizes in the range of 60-170 μm	822:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	6	37	theme	MC3T3	933:937	arg1	cells					939:943	MC3T3 cells	933:943	MC3T3 cells	933:943	Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture.					
34785622	5	38	dep	%	790:790	arg1	86					788:789	86	788:789	86	788:789	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	39	from	sizes	827:831	arg1	range					840:844	the range	836:844	the range of 60-170 μm	836:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	1	40	theme	natural	120:126	arg1	repair					128:133	natural repair	120:133	natural repair	120:133	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	40	theme	natural	120:126	arg1	processes					153:161	long-standing processes	139:161	long-standing processes that can lead to uneven new tissue growth	139:203	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	1	40	theme	natural	120:126	arg1	regeneration					103:114	Bone regeneration	98:114	Bone regeneration	98:114	Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth.					
34785622	4	41	theme	present	560:566	arg1	work					568:571	The present work	556:571	The present work	556:571	The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering.					
34785622	2	42	theme	major	330:334	arg1	burdens					336:342	the major burdens	326:342	the major burdens involved in this process	326:367	By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process.					
34785622	5	43	theme	obtained	747:754	arg1	scaffolds					756:764	the obtained scaffolds	743:764	the obtained scaffolds	743:764	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	5	44	from	interconnectivity	793:809	arg1	range					840:844	the range	836:844	the range of 60-170 μm	836:857	Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60-170 μm, and a stiffness ranging from 1 to 2 MPa.					
34785622	0	45	dep	scaffolds	26:34	arg1	coated					36:41	coated	36:41	scaffolds coated with silk fibroin: a bone tissue engineering approach	26:95	Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach.					
34785622	4	46	theme	silk	652:655	arg1	fibroin					657:663	silk fibroin	652:663	silk fibroin	652:663	The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering.					
34785622	0	47	theme	bone	64:67	arg1	approach					88:95	a bone tissue engineering approach	62:95	a bone tissue engineering approach	62:95	Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach.					
34785622	8	48	theme	developed	1212:1220	arg1	structures					1222:1231	the developed structures	1208:1231	the developed structures	1208:1231	These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.					
34785622	8	48	theme	developed	1212:1220	arg1	candidates					1246:1255	suitable candidates	1237:1255	suitable candidates	1237:1255	These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.					
34785622	3	49	theme	regeneration	532:543	arg1	solutions					545:553	bone regeneration solutions	527:553	bone regeneration solutions	527:553	Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions.					
34785622	4	50	theme	tissue	703:708	arg1	engineering					710:720	bone tissue engineering	698:720	bone tissue engineering	698:720	The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering.					
34785622	8	51	theme	bone	1268:1271	arg1	engineering					1280:1290	bone tissue engineering	1268:1290	bone tissue engineering	1268:1290	These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.					
32565421	0	0	link	adipose-derived	93:107	arg1	sheet					119:123	adipose-derived stem cell sheet	93:123	adipose-derived stem cell sheet	93:123	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	10	1	theme	network	1611:1617	arg1	formation					1619:1627	enhanced capillary network formation	1592:1627	enhanced capillary network formation around the sheet	1592:1644	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	3	2	theme	collagen-hADSC	540:553	arg1	sheet					567:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	1	3	theme	islet	274:278	arg1	stability					286:294	islet graft stability	274:294	islet graft stability	274:294	OBJECTIVE Esterified collagen (EC) can be functionalized with heparin to enhance islet graft stability.					
32565421	8	4	theme	EC-Hep	1214:1219	arg1	composite					1221:1229	The EC-Hep composite	1210:1229	RESULTS The EC-Hep composite	1202:1229	RESULTS The EC-Hep composite allowed sustained release of growth factors.					
32565421	10	5	theme	glucose	1489:1495	arg1	control					1497:1503	stable long-term glucose control	1472:1503	stable long-term glucose control by the graft	1472:1516	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	3	6	theme	-islet	560:565	arg1	sheet					567:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	1	7	theme	Esterified	203:212	arg1	collagen					214:221	OBJECTIVE Esterified collagen	193:221	OBJECTIVE Esterified collagen (EC)	193:226	OBJECTIVE Esterified collagen (EC) can be functionalized with heparin to enhance islet graft stability.					
32565421	1	7	theme	Esterified	203:212	arg1	EC					224:225	EC	224:225	EC	224:225	OBJECTIVE Esterified collagen (EC) can be functionalized with heparin to enhance islet graft stability.					
32565421	7	8	theme	β-Cell	1128:1133	arg1	mass					1135:1138	β-Cell mass	1128:1138	β-Cell mass	1128:1138	β-Cell mass and vascular densities were assessed by immunohistochemistry.					
32565421	10	9	theme	stable	1472:1477	arg1	control					1497:1503	stable long-term glucose control	1472:1503	stable long-term glucose control by the graft	1472:1516	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	2	10	theme	adipose-derived	330:344	arg1	hADSCs					358:363	hADSCs	358:363	hADSCs	358:363	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	2	10	theme	adipose-derived	330:344	arg1	cells					351:355	human adipose-derived stem cells	324:355	human adipose-derived stem cells (hADSCs)	324:364	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	8	11	theme	growth	1260:1265	arg1	factors					1267:1273	growth factors	1260:1273	growth factors	1260:1273	RESULTS The EC-Hep composite allowed sustained release of growth factors.					
32565421	9	12	theme	growth	1289:1294	arg1	factors					1296:1302	growth factors	1289:1302	growth factors	1289:1302	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	3	13	theme	islet	584:588	arg1	engraftment					590:600	islet engraftment	584:600	islet engraftment	584:600	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	6	14	theme	Islet	997:1001	arg1	transplantation					1003:1017	Islet transplantation	997:1017	Islet transplantation	997:1017	Islet transplantation was performed in mice, and graft function and survival were monitored by measuring the blood glucose levels.					
32565421	4	15	dep	DESIGN	612:617	arg1	designed					646:653	designed	646:653	was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice	642:772	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	5	16	theme	islet	845:849	arg1	function					851:858	islet function	845:858	islet function	845:858	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	5	17	theme	glucose-stimulated	876:893	arg1	secretion					903:911	glucose-stimulated insulin secretion	876:911	glucose-stimulated insulin secretion test	876:916	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	9	18	from	Secretion	1276:1284	arg1	sheet					1345:1349	the HCA-islet sheet	1331:1349	the HCA-islet sheet	1331:1349	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	0	19	theme	engineered	163:172	arg1	scaffold					183:190	engineered collagen scaffold	163:190	engineered collagen scaffold	163:190	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	9	20	theme	functionality	1314:1326	arg1	Secretion					1276:1284	Secretion	1276:1284	Secretion of growth factors and islet functionality in the HCA-islet sheet	1276:1349	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	4	21	theme	diabetic	752:759	arg1	mice					769:772	diabetic athymic mice	752:772	diabetic athymic mice	752:772	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	5	22	theme	hADSC-cocultured	785:800	arg1	islets					802:807	the hADSC-cocultured islets	781:807	the hADSC-cocultured islets	781:807	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	6	23	theme	graft	1046:1050	arg1	function					1052:1059	graft function	1046:1059	graft function	1046:1059	Islet transplantation was performed in mice, and graft function and survival were monitored by measuring the blood glucose levels.					
32565421	4	24	theme	subcutaneous	714:725	arg1	transplantation					733:747	subcutaneous islet transplantation	714:747	subcutaneous islet transplantation in diabetic athymic mice	714:772	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	0	25	theme	cells	83:87	arg1	transplantation					47:61	subcutaneous transplantation	34:61	subcutaneous transplantation of pancreatic islet cells	34:87	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	0	25	theme	cells	83:87	arg1	sheet					119:123	adipose-derived stem cell sheet	93:123	adipose-derived stem cell sheet	93:123	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	11	26	theme	diabetes	1865:1872	arg1	mellitus					1874:1881	diabetes mellitus	1865:1881	diabetes mellitus	1865:1881	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	0	27	theme	adipose-derived	93:107	arg1	sheet					119:123	adipose-derived stem cell sheet	93:123	adipose-derived stem cell sheet	93:123	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	8	28	dep	RESULTS	1202:1208	arg1	composite					1221:1229	The EC-Hep composite	1210:1229	RESULTS The EC-Hep composite	1202:1229	RESULTS The EC-Hep composite allowed sustained release of growth factors.					
32565421	0	29	theme	cell	114:117	arg1	sheet					119:123	adipose-derived stem cell sheet	93:123	adipose-derived stem cell sheet	93:123	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	6	30	theme	glucose	1112:1118	arg1	levels					1120:1125	the blood glucose levels	1102:1125	the blood glucose levels	1102:1125	Islet transplantation was performed in mice, and graft function and survival were monitored by measuring the blood glucose levels.					
32565421	0	31	theme	Long-term	0:8	arg1	reversal					10:17	Long-term reversal	0:17	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.	0:191	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	5	32	theme	protein	982:988	arg1	array					990:994	protein array	982:994	protein array	982:994	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	2	33	theme	cell	448:451	arg1	protection					453:462	cell protection	448:462	cell protection	448:462	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	9	34	dep	increased	1370:1378	arg1	compared					1380:1387	compared	1380:1387	compared with the control groups of islets alone or combined with native collagen	1380:1460	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	10	35	theme	sheet	1579:1583	arg1	transplantation					1550:1564	subcutaneous transplantation	1537:1564	subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet	1537:1644	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	9	36	theme	islets	1416:1421	arg1	islets					1416:1421	islets	1416:1421	islets alone or combined with native collagen	1416:1460	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	9	36	theme	islets	1416:1421	arg1	groups					1406:1411	the control groups	1394:1411	the control groups of islets alone or combined with native collagen	1394:1460	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	5	37	theme	insulin	895:901	arg1	secretion					903:911	glucose-stimulated insulin secretion	876:911	glucose-stimulated insulin secretion test	876:916	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	10	38	theme	capillary	1601:1609	arg1	formation					1619:1627	enhanced capillary network formation	1592:1627	enhanced capillary network formation around the sheet	1592:1644	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	0	39	theme	pancreatic	66:75	arg1	cells					83:87	pancreatic islet cells	66:87	pancreatic islet cells	66:87	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	5	40	theme	EC-Hep	828:833	arg1	scaffold					835:842	the EC-Hep scaffold	824:842	the EC-Hep scaffold	824:842	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	11	41	theme	clinical	1813:1820	arg1	transplantation					1828:1842	clinical islet transplantation	1813:1842	clinical islet transplantation for the treatment of diabetes mellitus	1813:1881	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	11	42	theme	sheet	1712:1716	arg1	potential					1685:1693	the potential	1681:1693	the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner	1681:1800	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	5	43	theme	secretion	903:911	arg1	test					913:916	glucose-stimulated insulin secretion test	876:916	glucose-stimulated insulin secretion test	876:916	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	3	44	theme	heparin-esterified	521:538	arg1	sheet					567:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	11	45	theme	dose-dependent	1780:1793	arg1	manner					1795:1800	a hADSC dose-dependent manner	1772:1800	a hADSC dose-dependent manner	1772:1800	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	11	46	dep	CONCLUSIONS	1647:1657	arg1	indicate					1672:1679	indicate	1672:1679	indicate	1672:1679	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	4	47	theme	RESEARCH	603:610	arg1	DESIGN					612:617	RESEARCH DESIGN	603:617	RESEARCH DESIGN	603:617	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	7	48	theme	vascular	1144:1151	arg1	densities					1153:1161	vascular densities	1144:1161	vascular densities	1144:1161	β-Cell mass and vascular densities were assessed by immunohistochemistry.					
32565421	3	49	theme	sheet	567:571	arg1	potential					497:505	the therapeutic potential	481:505	the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment	481:600	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	1	50	theme	graft	280:284	arg1	stability					286:294	islet graft stability	274:294	islet graft stability	274:294	OBJECTIVE Esterified collagen (EC) can be functionalized with heparin to enhance islet graft stability.					
32565421	2	51	theme	Growth	297:302	arg1	factors					304:310	Growth factors	297:310	Growth factors secreted by human adipose-derived stem cells (hADSCs)	297:364	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	1	52	theme	OBJECTIVE	193:201	arg1	collagen					214:221	OBJECTIVE Esterified collagen	193:221	OBJECTIVE Esterified collagen (EC)	193:226	OBJECTIVE Esterified collagen (EC) can be functionalized with heparin to enhance islet graft stability.					
32565421	1	52	theme	OBJECTIVE	193:201	arg1	EC					224:225	EC	224:225	EC	224:225	OBJECTIVE Esterified collagen (EC) can be functionalized with heparin to enhance islet graft stability.					
32565421	10	53	theme	long-term	1479:1487	arg1	control					1497:1503	stable long-term glucose control	1472:1503	stable long-term glucose control by the graft	1472:1516	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	2	54	theme	stem	346:349	arg1	hADSCs					358:363	hADSCs	358:363	hADSCs	358:363	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	2	54	theme	stem	346:349	arg1	cells					351:355	human adipose-derived stem cells	324:355	human adipose-derived stem cells (hADSCs)	324:364	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	8	55	theme	sustained	1239:1247	arg1	release					1249:1255	sustained release	1239:1255	sustained release of growth factors	1239:1273	RESULTS The EC-Hep composite allowed sustained release of growth factors.					
32565421	0	56	theme	surface-immobilized	131:149	arg1	heparin					151:157	surface-immobilized heparin	131:157	surface-immobilized heparin	131:157	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	2	57	theme	human	324:328	arg1	hADSCs					358:363	hADSCs	358:363	hADSCs	358:363	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	2	57	theme	human	324:328	arg1	cells					351:355	human adipose-derived stem cells	324:355	human adipose-derived stem cells (hADSCs)	324:364	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	9	58	theme	factors	1296:1302	arg1	Secretion					1276:1284	Secretion	1276:1284	Secretion of growth factors and islet functionality in the HCA-islet sheet	1276:1349	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	8	59	theme	factors	1267:1273	arg1	release					1249:1255	sustained release	1239:1255	sustained release of growth factors	1239:1273	RESULTS The EC-Hep composite allowed sustained release of growth factors.					
32565421	0	60	theme	collagen	174:181	arg1	scaffold					183:190	engineered collagen scaffold	163:190	engineered collagen scaffold	163:190	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	9	61	theme	islet	1308:1312	arg1	functionality					1314:1326	islet functionality	1308:1326	islet functionality	1308:1326	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	5	62	theme	culture	944:950	arg1	supernatants					952:963	the culture supernatants	940:963	the culture supernatants	940:963	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	4	63	theme	islet	727:731	arg1	transplantation					733:747	subcutaneous islet transplantation	714:747	subcutaneous islet transplantation in diabetic athymic mice	714:772	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	2	64	link	adipose-derived	330:344	arg1	hADSCs					358:363	hADSCs	358:363	hADSCs	358:363	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	2	64	link	adipose-derived	330:344	arg1	cells					351:355	human adipose-derived stem cells	324:355	human adipose-derived stem cells (hADSCs)	324:364	Growth factors secreted by human adipose-derived stem cells (hADSCs) can bind efficiently to EC-heparin (EC-Hep), which enhances revascularization and cell protection.					
32565421	10	65	theme	subcutaneous	1537:1548	arg1	transplantation					1550:1564	subcutaneous transplantation	1537:1564	subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet	1537:1644	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	0	66	theme	islet	77:81	arg1	cells					83:87	pancreatic islet cells	66:87	pancreatic islet cells	66:87	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	9	67	theme	HCA-islet	1335:1343	arg1	sheet					1345:1349	the HCA-islet sheet	1331:1349	the HCA-islet sheet	1331:1349	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	5	68	theme	growth	922:927	arg1	factors					929:935	growth factors	922:935	growth factors in the culture supernatants	922:963	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	0	69	theme	stem	109:112	arg1	sheet					119:123	adipose-derived stem cell sheet	93:123	adipose-derived stem cell sheet	93:123	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	11	70	theme	mellitus	1874:1881	arg1	treatment					1852:1860	the treatment	1848:1860	the treatment of diabetes mellitus	1848:1881	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	5	71	from	factors	929:935	arg1	supernatants					952:963	the culture supernatants	940:963	the culture supernatants	940:963	After the hADSC-cocultured islets were seeded in the EC-Hep scaffold, islet function was measured by glucose-stimulated insulin secretion test and growth factors in the culture supernatants were detected by protein array.					
32565421	9	72	theme	control	1398:1404	arg1	islets					1416:1421	islets	1416:1421	islets alone or combined with native collagen	1416:1460	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	9	72	theme	control	1398:1404	arg1	groups					1406:1411	the control groups	1394:1411	the control groups of islets alone or combined with native collagen	1394:1460	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	6	73	theme	blood	1106:1110	arg1	levels					1120:1125	the blood glucose levels	1102:1125	the blood glucose levels	1102:1125	Islet transplantation was performed in mice, and graft function and survival were monitored by measuring the blood glucose levels.					
32565421	4	74	theme	athymic	761:767	arg1	mice					769:772	diabetic athymic mice	752:772	diabetic athymic mice	752:772	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	0	75	theme	diabetes	22:29	arg1	reversal					10:17	Long-term reversal	0:17	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.	0:191	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	10	76	theme	HCA-islet	1569:1577	arg1	sheet					1579:1583	HCA-islet sheet	1569:1583	HCA-islet sheet	1569:1583	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	3	77	theme	therapeutic	485:495	arg1	potential					497:505	the therapeutic potential	481:505	the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment	481:600	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	0	78	theme	subcutaneous	34:45	arg1	transplantation					47:61	subcutaneous transplantation	34:61	subcutaneous transplantation of pancreatic islet cells	34:87	Long-term reversal of diabetes by subcutaneous transplantation of pancreatic islet cells and adipose-derived stem cell sheet using surface-immobilized heparin and engineered collagen scaffold.					
32565421	11	79	theme	hADSC	1774:1778	arg1	manner					1795:1800	a hADSC dose-dependent manner	1772:1800	a hADSC dose-dependent manner	1772:1800	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	10	80	theme	enhanced	1592:1599	arg1	formation					1619:1627	enhanced capillary network formation	1592:1627	enhanced capillary network formation around the sheet	1592:1644	In vivo, stable long-term glucose control by the graft was achieved after subcutaneous transplantation of HCA-islet sheet due to enhanced capillary network formation around the sheet.					
32565421	11	81	theme	islet	1822:1826	arg1	transplantation					1828:1842	clinical islet transplantation	1813:1842	clinical islet transplantation for the treatment of diabetes mellitus	1813:1881	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	3	82	theme	combined	512:519	arg1	sheet					567:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
32565421	9	83	theme	native	1446:1451	arg1	collagen					1453:1460	native collagen	1446:1460	native collagen	1446:1460	Secretion of growth factors and islet functionality in the HCA-islet sheet were significantly increased compared with the control groups of islets alone or combined with native collagen.					
32565421	11	84	theme	HCA-islet	1702:1710	arg1	sheet					1712:1716	the HCA-islet sheet	1698:1716	the HCA-islet sheet	1698:1716	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	4	85	from	transplantation	733:747	arg1	mice					769:772	diabetic athymic mice	752:772	diabetic athymic mice	752:772	RESEARCH DESIGN AND METHODS This study was designed to assess the efficiency of using EC-Hep as a scaffold for subcutaneous islet transplantation in diabetic athymic mice.					
32565421	11	86	theme	islet	1729:1733	arg1	revascularization					1735:1751	islet revascularization	1729:1751	islet revascularization	1729:1751	CONCLUSIONS The findings indicate the potential of the HCA-islet sheet to enhance islet revascularization and engraftment in a hADSC dose-dependent manner, following clinical islet transplantation for the treatment of diabetes mellitus.					
32565421	3	87	theme	HCA	556:558	arg1	sheet					567:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	a combined heparin-esterified collagen-hADSC (HCA)-islet sheet	510:571	We investigated the therapeutic potential of a combined heparin-esterified collagen-hADSC (HCA)-islet sheet to enhance islet engraftment.					
33757829	0	0	theme	polyvinylpyrrolidone-hyaluronic	100:130	arg1	nanoparticles					147:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	3	1	theme	1/6.25/3.75/15	663:676	arg1	ratio					654:658	the weight ratio	643:658	the weight ratio of 1/6.25/3.75/15 furnished the most	643:695	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	4	2	theme	marketed	842:849	arg1	Lipilow					860:866	Lipilow	860:866	Lipilow	860:866	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	4	2	theme	marketed	842:849	arg1	product					851:857	marketed product	842:857	marketed product (Lipilow)	842:867	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	4	3	from	powder	831:836	arg1	buffer					882:887	phosphate buffer pH = 6.8	872:896	phosphate buffer pH = 6.8	872:896	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	0	4	theme	electrospun	88:98	arg1	nanoparticles					147:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	3	5	theme	weight	647:652	arg1	ratio					654:658	the weight ratio	643:658	the weight ratio of 1/6.25/3.75/15 furnished the most	643:695	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	1	6	theme	aqueous	197:203	arg1	solubility					205:214	the aqueous solubility	193:214	the aqueous solubility of atorvastatin (AT)	193:235	The study was aimed to improve the aqueous solubility of atorvastatin (AT) and ameliorate permeability of metformin (MT) in a combination formulation, improving their oral bioavailability.					
33757829	8	7	theme	delivery	1451:1458	arg1	system					1460:1465	a promising drug delivery system	1434:1465	a promising drug delivery system for co-delivery of these drugs	1434:1496	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	0	8	theme	composite	137:145	arg1	nanoparticles					147:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	6	9	with	AUC	1078:1080	arg1	ES-NPs					1108:1113	ES-NPs	1108:1113	ES-NPs in rats	1108:1121	As compared with Lipilow, AUC (0-∞) and Cmax of AT with ES-NPs in rats were improved to 3.6-fold and 3.2-fold, respectively.					
33757829	4	10	theme	phosphate	872:880	arg1	buffer					882:887	phosphate buffer pH = 6.8	872:896	phosphate buffer pH = 6.8	872:896	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	0	11	theme	acid	132:135	arg1	nanoparticles					147:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles	88:159	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	3	12	theme	most	692:695	arg1	1/6.25/3.75/15					663:676	1/6.25/3.75/15 furnished the most	663:695	1/6.25/3.75/15 furnished the most	663:695	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	5	13	theme	MT	936:937	arg1	permeation					922:931	permeation	922:931	permeation of MT	922:937	Whereas, permeation of MT was 1.60-fold and 1.47-fold improved as compared with MT powder and marketed product (Glucophage), respectively.					
33757829	2	14	dep	in	545:546	arg1	vivo					548:551	vivo	548:551	vivo	548:551	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	6	15	theme	AT	1100:1101	arg1	0-∞					1083:1085	0-∞	1083:1085	0-∞	1083:1085	As compared with Lipilow, AUC (0-∞) and Cmax of AT with ES-NPs in rats were improved to 3.6-fold and 3.2-fold, respectively.					
33757829	6	15	theme	AT	1100:1101	arg1	Cmax					1092:1095	Cmax	1092:1095	Cmax	1092:1095	As compared with Lipilow, AUC (0-∞) and Cmax of AT with ES-NPs in rats were improved to 3.6-fold and 3.2-fold, respectively.					
33757829	6	15	theme	AT	1100:1101	arg1	AUC					1078:1080	AUC	1078:1080	AUC (0-∞)	1078:1086	As compared with Lipilow, AUC (0-∞) and Cmax of AT with ES-NPs in rats were improved to 3.6-fold and 3.2-fold, respectively.					
33757829	1	16	theme	atorvastatin	219:230	arg1	solubility					205:214	the aqueous solubility	193:214	the aqueous solubility of atorvastatin (AT)	193:235	The study was aimed to improve the aqueous solubility of atorvastatin (AT) and ameliorate permeability of metformin (MT) in a combination formulation, improving their oral bioavailability.					
33757829	3	17	theme	furnished	678:686	arg1	1/6.25/3.75/15					663:676	1/6.25/3.75/15 furnished the most	663:695	1/6.25/3.75/15 furnished the most	663:695	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	6	18	from	ES-NPs	1108:1113	arg1	rats					1118:1121	rats	1118:1121	rats	1118:1121	As compared with Lipilow, AUC (0-∞) and Cmax of AT with ES-NPs in rats were improved to 3.6-fold and 3.2-fold, respectively.					
33757829	8	19	theme	MT	1395:1396	arg1	solubility					1336:1345	the solubility	1332:1345	the solubility of AT (a BCS class II drug)	1332:1373	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	8	19	theme	MT	1395:1396	arg1	permeability					1379:1390	permeability	1379:1390	permeability of MT (a BCS class III drug)	1379:1419	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	3	20	theme	release	723:729	arg1	rate					731:734	release rate	723:734	release rate	723:734	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	2	21	theme	acid	414:417	arg1	nanoparticles					430:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles	351:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles	351:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	1	22	theme	combination	288:298	arg1	formulation					300:310	a combination formulation	286:310	a combination formulation	286:310	The study was aimed to improve the aqueous solubility of atorvastatin (AT) and ameliorate permeability of metformin (MT) in a combination formulation, improving their oral bioavailability.					
33757829	4	23	theme	AT	828:829	arg1	powder					831:836	AT powder	828:836	AT powder	828:836	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	3	24	theme	trialed	575:581	arg1	formulations					583:594	the trialed formulations	571:594	the trialed formulations	571:594	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	2	25	theme	hyaluronic	403:412	arg1	HA					420:421	HA	420:421	HA	420:421	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	25	theme	hyaluronic	403:412	arg1	acid					414:417	hyaluronic acid	403:417	hyaluronic acid (HA)	403:422	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	0	26	theme	biopharmaceutical	24:40	arg1	performance					42:52	biopharmaceutical performance	24:52	biopharmaceutical performance	24:52	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	3	27	theme	rate	731:734	arg1	results					736:742	solubility and release rate results	708:742	solubility and release rate results	708:742	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	2	28	dep	in	531:532	arg1	vitro					534:538	vitro	534:538	vitro	534:538	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	3	29	theme	solubility	708:717	arg1	results					736:742	solubility and release rate results	708:742	solubility and release rate results	708:742	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	8	30	theme	class	1405:1409	arg1	MT					1395:1396	MT	1395:1396	MT (a BCS class III drug)	1395:1419	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	8	30	theme	class	1405:1409	arg1	drug					1415:1418	a BCS class III drug	1399:1418	a BCS class III drug	1399:1418	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	2	31	theme	based	424:428	arg1	nanoparticles					430:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles	351:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles	351:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	3	32	from	ratio	654:658	arg1	PVP					628:630	PVP	628:630	PVP	628:630	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	3	32	from	ratio	654:658	arg1	HA					637:638	HA	637:638	HA	637:638	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	3	32	from	ratio	654:658	arg1	MT					624:625	MT	624:625	MT	624:625	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	3	32	from	ratio	654:658	arg1	AT					620:621	AT	620:621	AT	620:621	Among the trialed formulations, a sample consisting of AT, MT, PVP, and HA at the weight ratio of 1/6.25/3.75/15 furnished the most satisfying solubility and release rate results.					
33757829	8	33	theme	AT	1350:1351	arg1	solubility					1336:1345	the solubility	1332:1345	the solubility of AT (a BCS class II drug)	1332:1373	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	8	33	theme	AT	1350:1351	arg1	permeability					1379:1390	permeability	1379:1390	permeability of MT (a BCS class III drug)	1379:1419	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	8	34	theme	drug	1446:1449	arg1	system					1460:1465	a promising drug delivery system	1434:1465	a promising drug delivery system for co-delivery of these drugs	1434:1496	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	1	35	theme	oral	329:332	arg1	bioavailability					334:348	their oral bioavailability	323:348	their oral bioavailability	323:348	The study was aimed to improve the aqueous solubility of atorvastatin (AT) and ameliorate permeability of metformin (MT) in a combination formulation, improving their oral bioavailability.					
33757829	5	36	theme	MT	993:994	arg1	powder					996:1001	MT powder	993:1001	MT powder	993:1001	Whereas, permeation of MT was 1.60-fold and 1.47-fold improved as compared with MT powder and marketed product (Glucophage), respectively.					
33757829	2	37	dep	physicochemical	514:528	arg1	in					531:532	in	531:532	in	531:532	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	37	dep	physicochemical	514:528	arg1	in					545:546	in	545:546	in	545:546	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	38	theme	polyvinylpyrrolidone	372:391	arg1	nanoparticles					430:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles	351:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles	351:442	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	0	39	theme	atorvastatin	57:68	arg1	solubility					9:18	solubility	9:18	solubility	9:18	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	0	39	theme	atorvastatin	57:68	arg1	performance					42:52	biopharmaceutical performance	24:52	biopharmaceutical performance	24:52	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	6	40	with	Cmax	1092:1095	arg1	ES-NPs					1108:1113	ES-NPs	1108:1113	ES-NPs in rats	1108:1121	As compared with Lipilow, AUC (0-∞) and Cmax of AT with ES-NPs in rats were improved to 3.6-fold and 3.2-fold, respectively.					
33757829	4	41	from	product	851:857	arg1	buffer					882:887	phosphate buffer pH = 6.8	872:896	phosphate buffer pH = 6.8	872:896	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	4	42	theme	AT	808:809	arg1	solubility					794:803	approximately 10.3-fold and 3.6-fold solubility	757:803	approximately 10.3-fold and 3.6-fold solubility of AT	757:809	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	2	43	theme	loaded	365:370	arg1	PVP					394:396	PVP	394:396	PVP	394:396	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	43	theme	loaded	365:370	arg1	polyvinylpyrrolidone					372:391	Several AT-MT loaded polyvinylpyrrolidone	351:391	Several AT-MT loaded polyvinylpyrrolidone (PVP)	351:397	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	8	44	theme	BCS	1401:1403	arg1	MT					1395:1396	MT	1395:1396	MT (a BCS class III drug)	1395:1419	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	8	44	theme	BCS	1401:1403	arg1	drug					1415:1418	a BCS class III drug	1399:1418	a BCS class III drug	1399:1418	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	1	45	from	permeability	252:263	arg1	formulation					300:310	a combination formulation	286:310	a combination formulation	286:310	The study was aimed to improve the aqueous solubility of atorvastatin (AT) and ameliorate permeability of metformin (MT) in a combination formulation, improving their oral bioavailability.					
33757829	5	46	theme	marketed	1007:1014	arg1	product					1016:1022	marketed product	1007:1022	marketed product (Glucophage)	1007:1035	Whereas, permeation of MT was 1.60-fold and 1.47-fold improved as compared with MT powder and marketed product (Glucophage), respectively.					
33757829	5	46	theme	marketed	1007:1014	arg1	Glucophage					1025:1034	Glucophage	1025:1034	Glucophage	1025:1034	Whereas, permeation of MT was 1.60-fold and 1.47-fold improved as compared with MT powder and marketed product (Glucophage), respectively.					
33757829	4	47	theme	10.3-fold	771:779	arg1	solubility					794:803	approximately 10.3-fold and 3.6-fold solubility	757:803	approximately 10.3-fold and 3.6-fold solubility of AT	757:809	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	2	48	theme	AT-MT	359:363	arg1	PVP					394:396	PVP	394:396	PVP	394:396	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	48	theme	AT-MT	359:363	arg1	polyvinylpyrrolidone					372:391	Several AT-MT loaded polyvinylpyrrolidone	351:391	Several AT-MT loaded polyvinylpyrrolidone (PVP)	351:397	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	8	49	theme	class	1360:1364	arg1	AT					1350:1351	AT	1350:1351	AT (a BCS class II drug)	1350:1373	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	8	49	theme	class	1360:1364	arg1	drug					1369:1372	a BCS class II drug	1354:1372	a BCS class II drug	1354:1372	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	2	50	theme	Several	351:357	arg1	PVP					394:396	PVP	394:396	PVP	394:396	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	50	theme	Several	351:357	arg1	polyvinylpyrrolidone					372:391	Several AT-MT loaded polyvinylpyrrolidone	351:391	Several AT-MT loaded polyvinylpyrrolidone (PVP)	351:397	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	2	51	theme	physicochemical	514:528	arg1	parameters					553:562	physicochemical, in vitro, and in vivo parameters	514:562	physicochemical, in vitro, and in vivo parameters	514:562	Several AT-MT loaded polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) based nanoparticles were prepared through electrospraying method (ES-NPs), and tested for physicochemical, in vitro, and in vivo parameters.					
33757829	8	52	theme	BCS	1356:1358	arg1	class					1360:1364	BCS class II	1356:1367	a BCS class II drug	1354:1372	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	4	53	theme	3.6-fold	785:792	arg1	solubility					794:803	approximately 10.3-fold and 3.6-fold solubility	757:803	approximately 10.3-fold and 3.6-fold solubility of AT	757:809	It enhanced approximately 10.3-fold and 3.6-fold solubility of AT as compared with AT powder and marketed product (Lipilow) in phosphate buffer pH = 6.8, respectively.					
33757829	8	54	theme	drugs	1492:1496	arg1	co-delivery					1471:1481	co-delivery	1471:1481	co-delivery of these drugs	1471:1496	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
33757829	1	55	theme	metformin	268:276	arg1	permeability					252:263	permeability	252:263	permeability of metformin (MT) in a combination formulation	252:310	The study was aimed to improve the aqueous solubility of atorvastatin (AT) and ameliorate permeability of metformin (MT) in a combination formulation, improving their oral bioavailability.					
33757829	0	56	theme	metformin	74:82	arg1	solubility					9:18	solubility	9:18	solubility	9:18	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	0	56	theme	metformin	74:82	arg1	performance					42:52	biopharmaceutical performance	24:52	biopharmaceutical performance	24:52	Enhanced solubility and biopharmaceutical performance of atorvastatin and metformin via electrospun polyvinylpyrrolidone-hyaluronic acid composite nanoparticles.					
33757829	7	57	theme	MT	1239:1240	arg1	AUC					1217:1219	AUC (0-∞)	1217:1225	AUC (0-∞)	1217:1225	Similarly, as compared with Glucophage, AUC (0-∞) and Cmax of MT were improved to 2.3-fold and 1.8-fold, respectively.					
33757829	7	57	theme	MT	1239:1240	arg1	Cmax					1231:1234	Cmax	1231:1234	Cmax	1231:1234	Similarly, as compared with Glucophage, AUC (0-∞) and Cmax of MT were improved to 2.3-fold and 1.8-fold, respectively.					
33757829	8	58	theme	promising	1436:1444	arg1	system					1460:1465	a promising drug delivery system	1434:1465	a promising drug delivery system for co-delivery of these drugs	1434:1496	Thus, ES-NPs significantly enhanced the solubility of AT (a BCS class II drug) and permeability of MT (a BCS class III drug) and might be a promising drug delivery system for co-delivery of these drugs.					
32684251	8	0	theme	acids	852:856	arg1	concentrations					763:776	The lowest fecal concentrations	746:776	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids	746:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	2	1	theme	fecal	295:299	arg1	microbiota					301:310	the fecal microbiota	291:310	the fecal microbiota of horses	291:320	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	12	2	theme	microbiota	1484:1493	arg1	clostridia					1404:1413	clostridia	1404:1413	clostridia	1404:1413	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	12	2	theme	microbiota	1484:1493	arg1	component					1456:1464	a key component	1450:1464	a key component of the intestinal microbiota in horses	1450:1503	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	13	3	theme	further	1672:1678	arg1	investigation					1680:1692	further investigation	1672:1692	further investigation	1672:1692	However, this finding requires further investigation, particularly with regard to the Clostridium species that have been promoted by dietary cellobiose.					
32684251	10	4	theme	relative	1146:1153	arg1	abundance					1155:1163	the relative abundance	1142:1163	the relative abundance of Bacteroidetes	1142:1180	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	1	5	theme	different	146:154	arg1	species					163:169	different animal species	146:169	different animal species	146:169	Cellobiose is a disaccharide with potential prebiotic effects, as demonstrated in different animal species, but not yet in horses.					
32684251	2	6	from	impact	259:264	arg1	microbiota					301:310	the fecal microbiota	291:310	the fecal microbiota of horses	291:320	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	10	7	located	observed	1197:1204	arg2	decrease					1130:1137	a dose-dependent decrease	1113:1137	a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035)	1113:1191	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	10	7	located	observed	1197:1204	arg2	P =					1183:1185	P =	1183:1185	P =	1183:1185	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	10	7	located	observed	1197:1204	arg1	addition					1103:1110	addition	1103:1110	addition	1103:1110	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	0	8	from	Impact	0:5	arg1	Microbiota					42:51	the Fecal Microbiota	32:51	the Fecal Microbiota of Horses	32:61	Impact of Dietary Cellobiose on the Fecal Microbiota of Horses.					
32684251	8	9	theme	quadratic	919:927	arg1	day					914:916	day	914:916	day (quadratic effect: P < .05)	914:944	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	8	9	theme	quadratic	919:927	arg1	<					939:939	quadratic effect: P < .05	919:943	quadratic effect: P < .05	919:943	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	2	10	theme	study	237:241	arg1	It					195:196	It	195:196	It	195:196	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	2	10	theme	study	237:241	arg1	aim					218:220	the aim	214:220	the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses	214:320	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	8	11	theme	lowest	750:755	arg1	concentrations					763:776	The lowest fecal concentrations	746:776	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids	746:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	12	12	theme	prebiotic	1598:1606	arg1	effects					1608:1614	beneficial and potentially prebiotic effects	1571:1614	beneficial and potentially prebiotic effects of cellobiose in horses	1571:1638	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	8	13	theme	acid	818:821	arg1	concentrations					763:776	The lowest fecal concentrations	746:776	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids	746:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	5	14	theme	feeding	507:513	arg1	periods					515:521	the feeding periods	503:521	the feeding periods	503:521	At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition.					
32684251	2	15	theme	present	229:235	arg1	study					237:241	the present study	225:241	the present study	225:241	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	4	16	dep	14 days	475:481	arg1	each					483:486	each	483:486	each	483:486	The animals received a diet without or with 10 g and 20 g cellobiose per day for 14 days each.					
32684251	5	17	theme	periods	515:521	arg1	end					496:498	the end	492:498	the end of the feeding periods	492:521	At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition.					
32684251	11	18	theme	Coriobacteriales	1238:1253	arg1	increase					1226:1233	the increase	1222:1233	the increase of Coriobacteriales and Clostridium	1222:1269	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	1	19	theme	animal	156:161	arg1	species					163:169	different animal species	146:169	different animal species	146:169	Cellobiose is a disaccharide with potential prebiotic effects, as demonstrated in different animal species, but not yet in horses.					
32684251	5	20	theme	fresh	524:528	arg1	samples					536:542	fresh fecal samples	524:542	fresh fecal samples	524:542	At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition.					
32684251	9	21	theme	<	1045:1045	arg1	P					1043:1043	P < .001	1043:1050	P < .001	1043:1050	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected.					
32684251	9	21	theme	<	1045:1045	arg1	Coriobacteriales					1025:1040	Coriobacteriales	1025:1040	Coriobacteriales (P < .001)	1025:1051	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected.					
32684251	12	22	from	component	1456:1464	arg1	horses					1498:1503	horses	1498:1503	horses	1498:1503	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	12	23	theme	beneficial	1571:1580	arg1	effects					1608:1614	beneficial and potentially prebiotic effects	1571:1614	beneficial and potentially prebiotic effects of cellobiose in horses	1571:1638	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	6	24	used	used	681:684	arg2	sequencing					666:675	16S rRNA gene sequencing	652:675	16S rRNA gene sequencing	652:675	For the microbiota analysis, 16S rRNA gene sequencing was used.					
32684251	1	25	with	disaccharide	80:91	arg1	effects					118:124	potential prebiotic effects	98:124	potential prebiotic effects	98:124	Cellobiose is a disaccharide with potential prebiotic effects, as demonstrated in different animal species, but not yet in horses.					
32684251	5	26	theme	fecal	530:534	arg1	samples					536:542	fresh fecal samples	524:542	fresh fecal samples	524:542	At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition.					
32684251	11	27	theme	saccharolytic	1377:1389	arg1	activity					1391:1398	saccharolytic activity	1377:1398	saccharolytic activity	1377:1398	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	8	28	theme	D-lactate	781:789	arg1	concentrations					763:776	The lowest fecal concentrations	746:776	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids	746:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	3	29	theme	healthy	329:335	arg1	horses					343:348	Eight healthy adult horses	323:348	Eight healthy adult horses	323:348	Eight healthy adult horses and two ponies were included in this study.					
32684251	8	30	theme	P	937:937	arg1	day					914:916	day	914:916	day (quadratic effect: P < .05)	914:944	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	8	30	theme	P	937:937	arg1	<					939:939	quadratic effect: P < .05	919:943	quadratic effect: P < .05	919:943	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	11	31	from	fermentation	1293:1304	arg1	intestine					1334:1342	the equine intestine	1323:1342	the equine intestine	1323:1342	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	8	32	theme	acid	802:805	arg1	concentrations					763:776	The lowest fecal concentrations	746:776	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids	746:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	2	33	theme	horses	315:320	arg1	microbiota					301:310	the fecal microbiota	291:310	the fecal microbiota of horses	291:320	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	0	34	theme	Cellobiose	18:27	arg1	Impact					0:5	Impact	0:5	Impact of Dietary Cellobiose on the Fecal Microbiota of Horses.	0:62	Impact of Dietary Cellobiose on the Fecal Microbiota of Horses.					
32684251	5	35	theme	microbial	600:608	arg1	composition					610:620	the microbial composition	596:620	the microbial composition	596:620	At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition.					
32684251	11	36	theme	groups	1364:1369	arg1	members					1348:1354	members	1348:1354	members of both groups	1348:1369	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	8	37	theme	propionic	792:800	arg1	acid					802:805	propionic acid	792:805	propionic acid	792:805	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	0	38	theme	Dietary	10:16	arg1	Cellobiose					18:27	Dietary Cellobiose	10:27	Dietary Cellobiose	10:27	Impact of Dietary Cellobiose on the Fecal Microbiota of Horses.					
32684251	8	39	theme	total	828:832	arg1	acids					852:856	total short-chain fatty acids	828:856	total short-chain fatty acids	828:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	8	40	theme	i-valeric	808:816	arg1	acid					818:821	i-valeric acid	808:821	i-valeric acid	808:821	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	9	41	theme	dose-dependent	949:962	arg1	increase					964:971	A dose-dependent increase	947:971	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031)	947:1079	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected.					
32684251	2	42	theme	cellobiose	277:286	arg1	impact					259:264	the impact	255:264	the impact of dietary cellobiose on the fecal microbiota of horses	255:320	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	8	43	theme	fecal	757:761	arg1	concentrations					763:776	The lowest fecal concentrations	746:776	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids	746:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	11	44	theme	Clostridium	1259:1269	arg1	increase					1226:1233	the increase	1222:1233	the increase of Coriobacteriales and Clostridium	1222:1269	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	12	45	from	increase	1519:1526	arg1	feces					1550:1554	the feces	1546:1554	the feces	1546:1554	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	9	46	theme	relative	980:987	arg1	abundance					989:997	the relative abundance	976:997	the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031)	976:1079	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected.					
32684251	3	47	theme	adult	337:341	arg1	horses					343:348	Eight healthy adult horses	323:348	Eight healthy adult horses	323:348	Eight healthy adult horses and two ponies were included in this study.					
32684251	12	48	theme	cellobiose	1619:1628	arg1	effects					1608:1614	beneficial and potentially prebiotic effects	1571:1614	beneficial and potentially prebiotic effects of cellobiose in horses	1571:1638	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	5	49	theme	bacterial	570:578	arg1	metabolites					580:590	bacterial metabolites	570:590	bacterial metabolites	570:590	At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition.					
32684251	6	50	theme	gene	661:664	arg1	sequencing					666:675	16S rRNA gene sequencing	652:675	16S rRNA gene sequencing	652:675	For the microbiota analysis, 16S rRNA gene sequencing was used.					
32684251	1	51	theme	potential	98:106	arg1	effects					118:124	potential prebiotic effects	98:124	potential prebiotic effects	98:124	Cellobiose is a disaccharide with potential prebiotic effects, as demonstrated in different animal species, but not yet in horses.					
32684251	0	52	theme	Fecal	36:40	arg1	Microbiota					42:51	the Fecal Microbiota	32:51	the Fecal Microbiota of Horses	32:61	Impact of Dietary Cellobiose on the Fecal Microbiota of Horses.					
32684251	12	53	theme	Clostridium	1531:1541	arg1	increase					1519:1526	the observed increase	1506:1526	the observed increase of Clostridium in the feces	1506:1554	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	8	54	theme	effect	929:934	arg1	day					914:916	day	914:916	day (quadratic effect: P < .05)	914:944	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	8	54	theme	effect	929:934	arg1	<					939:939	quadratic effect: P < .05	919:943	quadratic effect: P < .05	919:943	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	6	55	theme	rRNA	656:659	arg1	sequencing					666:675	16S rRNA gene sequencing	652:675	16S rRNA gene sequencing	652:675	For the microbiota analysis, 16S rRNA gene sequencing was used.					
32684251	1	56	theme	prebiotic	108:116	arg1	effects					118:124	potential prebiotic effects	98:124	potential prebiotic effects	98:124	Cellobiose is a disaccharide with potential prebiotic effects, as demonstrated in different animal species, but not yet in horses.					
32684251	12	57	theme	observed	1510:1517	arg1	increase					1519:1526	the observed increase	1506:1526	the observed increase of Clostridium in the feces	1506:1554	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	6	58	theme	16S	652:654	arg1	sequencing					666:675	16S rRNA gene sequencing	652:675	16S rRNA gene sequencing	652:675	For the microbiota analysis, 16S rRNA gene sequencing was used.					
32684251	2	59	theme	dietary	269:275	arg1	cellobiose					277:286	dietary cellobiose	269:286	dietary cellobiose	269:286	It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses.					
32684251	10	60	theme	Bacteroidetes	1168:1180	arg1	abundance					1155:1163	the relative abundance	1142:1163	the relative abundance of Bacteroidetes	1142:1180	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	9	61	theme	abundance	989:997	arg1	increase					964:971	A dose-dependent increase	947:971	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031)	947:1079	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected.					
32684251	9	62	theme	Firmicutes	1002:1011	arg1	abundance					989:997	the relative abundance	976:997	the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031)	976:1079	A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected.					
32684251	10	63	theme	abundance	1155:1163	arg1	P =					1183:1185	P =	1183:1185	P =	1183:1185	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	10	63	theme	abundance	1155:1163	arg1	decrease					1130:1137	a dose-dependent decrease	1113:1137	a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035)	1113:1191	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	8	64	theme	fatty	846:850	arg1	acids					852:856	total short-chain fatty acids	828:856	total short-chain fatty acids	828:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	10	65	theme	dose-dependent	1115:1128	arg1	P =					1183:1185	P =	1183:1185	P =	1183:1185	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	10	65	theme	dose-dependent	1115:1128	arg1	decrease					1130:1137	a dose-dependent decrease	1113:1137	a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035)	1113:1191	In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed.					
32684251	11	66	theme	bacterial	1283:1291	arg1	fermentation					1293:1304	a bacterial fermentation	1281:1304	a bacterial fermentation of cellobiose in the equine intestine	1281:1342	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	12	67	theme	intestinal	1473:1482	arg1	microbiota					1484:1493	the intestinal microbiota	1469:1493	the intestinal microbiota	1469:1493	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	0	68	theme	Horses	56:61	arg1	Microbiota					42:51	the Fecal Microbiota	32:51	the Fecal Microbiota of Horses	32:61	Impact of Dietary Cellobiose on the Fecal Microbiota of Horses.					
32684251	13	69	theme	dietary	1774:1780	arg1	cellobiose					1782:1791	dietary cellobiose	1774:1791	dietary cellobiose	1774:1791	However, this finding requires further investigation, particularly with regard to the Clostridium species that have been promoted by dietary cellobiose.					
32684251	8	70	theme	short-chain	834:844	arg1	acids					852:856	total short-chain fatty acids	828:856	total short-chain fatty acids	828:856	The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05).					
32684251	11	71	theme	cellobiose	1309:1318	arg1	fermentation					1293:1304	a bacterial fermentation	1281:1304	a bacterial fermentation of cellobiose in the equine intestine	1281:1342	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	12	72	theme	key	1452:1454	arg1	clostridia					1404:1413	clostridia	1404:1413	clostridia	1404:1413	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	12	72	theme	key	1452:1454	arg1	component					1456:1464	a key component	1450:1464	a key component of the intestinal microbiota in horses	1450:1503	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	6	73	theme	microbiota	631:640	arg1	analysis					642:649	the microbiota analysis	627:649	the microbiota analysis	627:649	For the microbiota analysis, 16S rRNA gene sequencing was used.					
32684251	12	74	from	effects	1608:1614	arg1	horses					1633:1638	horses	1633:1638	horses	1633:1638	As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses.					
32684251	11	75	theme	equine	1327:1332	arg1	intestine					1334:1342	the equine intestine	1323:1342	the equine intestine	1323:1342	In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity.					
32684251	13	76	theme	Clostridium	1727:1737	arg1	species					1739:1745	the Clostridium species	1723:1745	the Clostridium species that have been promoted by dietary cellobiose	1723:1791	However, this finding requires further investigation, particularly with regard to the Clostridium species that have been promoted by dietary cellobiose.					
32090504	6	0	theme	chemical	1207:1214	arg1	definition					1216:1225	its defined and tunable chemical definition	1183:1225	its defined and tunable chemical definition	1183:1225	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	2	1	theme	liver-derived	354:366	arg1	organoids					374:382	adult liver-derived liver organoids	348:382	adult liver-derived liver organoids	348:382	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	6	2	theme	clinical	1330:1337	arg1	use					1339:1341	clinical use	1330:1341	clinical use	1330:1341	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	3	3	theme	tumor	662:666	arg1	material					668:675	murine tumor material	655:675	murine tumor material	655:675	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	4	4	theme	CNF	833:835	arg1	hydrogel					838:845	a cellulose nanofibril (CNF) hydrogel	809:845	a cellulose nanofibril (CNF) hydrogel	809:845	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	2	5	theme	adult	348:352	arg1	organoids					374:382	adult liver-derived liver organoids	348:382	adult liver-derived liver organoids	348:382	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	1	6	from	applicable	304:313	arg1	setting					329:335	a clinical setting	318:335	a clinical setting	318:335	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	2	7	theme	hepatocyte-like	508:522	arg1	fate					524:527	a hepatocyte-like fate	506:527	a hepatocyte-like fate	506:527	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	2	8	link	liver-derived	354:366	arg1	organoids					374:382	adult liver-derived liver organoids	348:382	adult liver-derived liver organoids	348:382	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	6	9	theme	CNF	1232:1234	arg1	hydrogel					1236:1243	the CNF hydrogel	1228:1243	the CNF hydrogel	1228:1243	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	1	10	theme	tissue	273:278	arg1	production					280:289	tissue production	273:289	tissue production	273:289	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	1	11	from	setting	329:335	arg1	applicable					304:313	applicable	304:313	applicable	304:313	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	4	12	theme	cellulose	811:819	arg1	hydrogel					838:845	a cellulose nanofibril (CNF) hydrogel	809:845	a cellulose nanofibril (CNF) hydrogel	809:845	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	1	13	theme	3D	172:173	arg1	engineering					182:192	3D tissue engineering	172:192	3D tissue engineering	172:192	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	4	14	theme	alternative	907:917	arg1	scaffold					934:941	an alternative clinical grade scaffold	904:941	an alternative clinical grade scaffold	904:941	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	5	15	theme	equal	1079:1083	arg1	functionality					1098:1110	overall, either equal or improved, functionality	1063:1110	functionality	1098:1110	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	0	16	theme	Cellulose	0:8	arg1	Hydrogel					21:28	Cellulose Nanofibril Hydrogel	0:28	Cellulose Nanofibril Hydrogel	0:28	Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.					
32090504	1	17	theme	tissue	175:180	arg1	engineering					182:192	3D tissue engineering	172:192	3D tissue engineering	172:192	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	0	18	theme	Nanofibril	10:19	arg1	Hydrogel					21:28	Cellulose Nanofibril Hydrogel	0:28	Cellulose Nanofibril Hydrogel	0:28	Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.					
32090504	2	19	theme	genetic	430:436	arg1	stability					438:446	their genetic stability	424:446	their genetic stability	424:446	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	4	20	theme	nanofibril	821:830	arg1	hydrogel					838:845	a cellulose nanofibril (CNF) hydrogel	809:845	a cellulose nanofibril (CNF) hydrogel	809:845	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	1	21	theme	functional	102:111	arg1	tissue					119:124	functional liver tissue	102:124	functional liver tissue	102:124	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	3	22	attach	derived	642:648	arg2	MG					628:629	MG	628:629	MG	628:629	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	22	attach	derived	642:648	arg2	Matrigel					618:625	a basement membrane hydrogel like Matrigel	584:625	a basement membrane hydrogel like Matrigel (MG)	584:630	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	22	attach	derived	642:648	arg2	standard					542:549	standard	542:549	standard	542:549	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	22	attach	derived	642:648	arg1	material					668:675	murine tumor material	655:675	murine tumor material	655:675	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	22	attach	derived	642:648	arg2	applicable					791:800	applicable	791:800	applicable	791:800	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	5	23	theme	overall	1063:1069	arg1	functionality					1098:1110	overall, either equal or improved, functionality	1063:1110	functionality	1098:1110	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	1	24	theme	liver	113:117	arg1	tissue					119:124	functional liver tissue	102:124	functional liver tissue	102:124	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	6	25	theme	tunable	1199:1205	arg1	definition					1216:1225	its defined and tunable chemical definition	1183:1225	its defined and tunable chemical definition	1183:1225	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	1	26	theme	representative	203:216	arg1	models					223:228	representative cell models	203:228	representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting	203:335	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	3	27	theme	high	712:715	arg1	costs					717:721	high costs	712:721	high costs	712:721	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	6	28	theme	tissue	1291:1296	arg1	engineering					1298:1308	liver tissue engineering	1285:1308	liver tissue engineering	1285:1308	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	1	29	theme	cell	218:221	arg1	models					223:228	representative cell models	203:228	representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting	203:335	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	6	30	theme	liver	1285:1289	arg1	engineering					1298:1308	liver tissue engineering	1285:1308	liver tissue engineering	1285:1308	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	3	31	theme	basement	586:593	arg1	MG					628:629	MG	628:629	MG	628:629	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	31	theme	basement	586:593	arg1	standard					542:549	standard	542:549	standard	542:549	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	31	theme	basement	586:593	arg1	Matrigel					618:625	a basement membrane hydrogel like Matrigel	584:625	a basement membrane hydrogel like Matrigel (MG)	584:630	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	31	theme	basement	586:593	arg1	applicable					791:800	applicable	791:800	applicable	791:800	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	5	32	with	differentiation	1042:1056	arg1	functionality					1098:1110	overall, either equal or improved, functionality	1063:1110	functionality	1098:1110	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	2	33	theme	much	403:406	arg1	interest					408:415	much interest	403:415	much interest	403:415	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	0	34	theme	Hepatic	39:45	arg1	Differentiation					47:61	Hepatic Differentiation	39:61	Hepatic Differentiation of Human Liver Organoids	39:86	Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.					
32090504	4	35	theme	liver	960:964	arg1	organoids					966:974	liver organoids	960:974	liver organoids	960:974	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	3	36	theme	membrane	595:602	arg1	MG					628:629	MG	628:629	MG	628:629	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	36	theme	membrane	595:602	arg1	standard					542:549	standard	542:549	standard	542:549	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	36	theme	membrane	595:602	arg1	Matrigel					618:625	a basement membrane hydrogel like Matrigel	584:625	a basement membrane hydrogel like Matrigel (MG)	584:630	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	36	theme	membrane	595:602	arg1	applicable					791:800	applicable	791:800	applicable	791:800	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	37	theme	undefined	737:745	arg1	composition					747:757	an undefined composition	734:757	an undefined composition	734:757	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	2	38	theme	expansion	449:457	arg1	potential					459:467	expansion potential	449:467	expansion potential	449:467	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
32090504	5	39	theme	hepatocyte-like	1119:1133	arg1	cells					1135:1139	the hepatocyte-like cells	1115:1139	the hepatocyte-like cells	1115:1139	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	0	40	theme	Liver	72:76	arg1	Organoids					78:86	Human Liver Organoids	66:86	Human Liver Organoids	66:86	Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.					
32090504	6	41	theme	defined	1187:1193	arg1	definition					1216:1225	its defined and tunable chemical definition	1183:1225	its defined and tunable chemical definition	1183:1225	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	5	42	theme	cells	1135:1139	arg1	functionality					1098:1110	overall, either equal or improved, functionality	1063:1110	functionality	1098:1110	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	5	43	theme	mechanical	1003:1012	arg1	properties					1014:1023	its mechanical properties	999:1023	its mechanical properties	999:1023	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	4	44	theme	grade	928:932	arg1	scaffold					934:941	an alternative clinical grade scaffold	904:941	an alternative clinical grade scaffold	904:941	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	4	45	theme	clinical	919:926	arg1	scaffold					934:941	an alternative clinical grade scaffold	904:941	an alternative clinical grade scaffold	904:941	Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids.					
32090504	1	46	theme	clinical	320:327	arg1	setting					329:335	a clinical setting	318:335	a clinical setting	318:335	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	3	47	theme	murine	655:660	arg1	material					668:675	murine tumor material	655:675	murine tumor material	655:675	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	48	contain	possesses	724:732	arg1	standard					542:549	standard	542:549	standard	542:549	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	48	contain	possesses	724:732	arg1	MG					628:629	MG	628:629	MG	628:629	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	48	contain	possesses	724:732	arg2	composition					747:757	an undefined composition	734:757	an undefined composition	734:757	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	48	contain	possesses	724:732	arg1	Matrigel					618:625	a basement membrane hydrogel like Matrigel	584:625	a basement membrane hydrogel like Matrigel (MG)	584:630	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	48	contain	possesses	724:732	arg1	applicable					791:800	applicable	791:800	applicable	791:800	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	6	49	theme	viable	1256:1261	arg1	alternative					1263:1273	a viable alternative	1254:1273	a viable alternative to MG for liver tissue engineering	1254:1308	Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.					
32090504	1	50	theme	drug	139:142	arg1	testing					144:150	drug testing	139:150	drug testing	139:150	To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting.					
32090504	0	51	theme	Organoids	78:86	arg1	Differentiation					47:61	Hepatic Differentiation	39:61	Hepatic Differentiation of Human Liver Organoids	39:86	Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.					
32090504	3	52	theme	hydrogel	604:611	arg1	MG					628:629	MG	628:629	MG	628:629	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	52	theme	hydrogel	604:611	arg1	standard					542:549	standard	542:549	standard	542:549	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	52	theme	hydrogel	604:611	arg1	Matrigel					618:625	a basement membrane hydrogel like Matrigel	584:625	a basement membrane hydrogel like Matrigel (MG)	584:630	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	52	theme	hydrogel	604:611	arg1	applicable					791:800	applicable	791:800	applicable	791:800	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	0	53	theme	Human	66:70	arg1	Organoids					78:86	Human Liver Organoids	66:86	Human Liver Organoids	66:86	Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.					
32090504	5	54	theme	improved	1088:1095	arg1	functionality					1098:1110	overall, either equal or improved, functionality	1063:1110	functionality	1098:1110	The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG.					
32090504	3	55	theme	like	613:616	arg1	MG					628:629	MG	628:629	MG	628:629	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	55	theme	like	613:616	arg1	standard					542:549	standard	542:549	standard	542:549	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	55	theme	like	613:616	arg1	Matrigel					618:625	a basement membrane hydrogel like Matrigel	584:625	a basement membrane hydrogel like Matrigel (MG)	584:630	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	3	55	theme	like	613:616	arg1	applicable					791:800	applicable	791:800	applicable	791:800	The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable.					
32090504	2	56	theme	liver	368:372	arg1	organoids					374:382	adult liver-derived liver organoids	348:382	adult liver-derived liver organoids	348:382	Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate.					
33688735	0	0	theme	Diseases	86:93	arg1	melanocarpa					53:63	Aronia melanocarpa	46:63	Aronia melanocarpa of LPS-Induced Liver Diseases in Rats	46:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	7	1	theme	gut	1272:1274	arg1	microbiota					1276:1285	the gut microbiota	1268:1285	the gut microbiota of LPS-induced liver diseases	1268:1315	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	0	2	theme	Liver	80:84	arg1	Diseases					86:93	LPS-Induced Liver Diseases	68:93	LPS-Induced Liver Diseases in Rats	68:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	4	3	theme	proteins	530:537	arg1	expression					506:515	expression	506:515	expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways	506:610	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	3	4	theme	melanocarpa	332:342	arg1	extract					318:324	a rich-polyphenol extract	300:324	a rich-polyphenol extract of A. melanocarpa (AMPs)	300:349	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	4	5	theme	liver	469:473	arg1	index					499:503	liver function index	484:503	liver function index	484:503	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	5	theme	liver	469:473	arg1	disease					475:481	the anti-LPS-induced liver disease	448:481	the anti-LPS-induced liver disease	448:481	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	5	6	theme	proteins	949:956	arg1	expression					893:902	the expression	889:902	the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum	889:991	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	4	7	theme	real-time	718:726	arg1	reaction					745:752	quantitative real-time polymerase chain reaction	705:752	quantitative real-time polymerase chain reaction (qRT-PCR)	705:762	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	7	theme	real-time	718:726	arg1	qRT-PCR					755:761	qRT-PCR	755:761	qRT-PCR	755:761	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	8	theme	factors	553:559	arg1	expression					506:515	expression	506:515	expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways	506:610	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	5	9	theme	epithelial	918:927	arg1	proteins					949:956	intestinal epithelial cell tight junction proteins	907:956	intestinal epithelial cell tight junction proteins	907:956	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	0	10	from	melanocarpa	53:63	arg1	Rats					98:101	Rats	98:101	Rats	98:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	0	10	from	melanocarpa	53:63	arg1	Polyphenols					29:39	Polyphenols	29:39	Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats	29:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	4	11	theme	chain	739:743	arg1	reaction					745:752	quantitative real-time polymerase chain reaction	705:752	quantitative real-time polymerase chain reaction (qRT-PCR)	705:762	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	11	theme	chain	739:743	arg1	qRT-PCR					755:761	qRT-PCR	755:761	qRT-PCR	755:761	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	2	12	theme	microbiota	255:264	arg1	modulation					266:275	gut microbiota modulation	251:275	gut microbiota modulation	251:275	However, the mechanism by which this is achieved through gut microbiota modulation remains unclear.					
33688735	4	13	theme	inflammatory	540:551	arg1	factors					553:559	inflammatory factors	540:559	inflammatory factors	540:559	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	14	theme	16S	682:684	arg1	sequencing					691:700	16S rRNA sequencing	682:700	16S rRNA sequencing	682:700	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	6	15	theme	factors	1032:1038	arg1	levels					1009:1014	The expression levels	994:1014	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	994:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	2	16	theme	gut	251:253	arg1	modulation					266:275	gut microbiota modulation	251:275	gut microbiota modulation	251:275	However, the mechanism by which this is achieved through gut microbiota modulation remains unclear.					
33688735	4	17	theme	blot	641:644	arg1	analysis					646:653	western blot analysis	633:653	western blot analysis	633:653	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	3	18	theme	-induced	393:400	arg1	diseases					408:415	lipopolysaccharide (LPS)-induced liver diseases	369:415	lipopolysaccharide (LPS)-induced liver diseases	369:415	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	3	19	theme	A.	329:330	arg1	AMPs					345:348	AMPs	345:348	AMPs	345:348	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	3	19	theme	A.	329:330	arg1	melanocarpa					332:342	A. melanocarpa	329:342	A. melanocarpa (AMPs)	329:349	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	7	20	theme	intestinal	1227:1236	arg1	function					1246:1253	the intestinal barrier function	1223:1253	the intestinal barrier function	1223:1253	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	7	20	theme	intestinal	1227:1236	arg1	substance					1198:1206	a bioactive substance	1186:1206	a bioactive substance	1186:1206	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	6	21	dep	factors	1032:1038	arg1	TNF-α					1111:1115	TNF-α	1111:1115	TNF-α	1111:1115	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	factors					1032:1038	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	interleukin					1040:1050	interleukin 6	1040:1052	interleukin 6 (IL-6)	1040:1059	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	interleukin					1062:1072	interleukin 1β	1062:1075	interleukin 1β (IL-1β)	1062:1083	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	factor					1101:1106	tumor necrosis factor α	1086:1108	tumor necrosis factor α (TNF-α)	1086:1116	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	IL-6					1055:1058	IL-6	1055:1058	IL-6	1055:1058	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	mRNAs					1131:1135	related mRNAs	1123:1135	related mRNAs	1123:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	21	dep	factors	1032:1038	arg1	IL-1β					1078:1082	IL-1β	1078:1082	IL-1β	1078:1082	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	3	22	theme	liver	402:406	arg1	diseases					408:415	lipopolysaccharide (LPS)-induced liver diseases	369:415	lipopolysaccharide (LPS)-induced liver diseases	369:415	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	0	23	theme	Microbiota	4:13	arg1	Modulation					15:24	Gut Microbiota Modulation	0:24	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.	0:102	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	0	24	theme	LPS-Induced	68:78	arg1	Diseases					86:93	LPS-Induced Liver Diseases	68:93	LPS-Induced Liver Diseases in Rats	68:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	4	25	theme	quantitative	705:716	arg1	reaction					745:752	quantitative real-time polymerase chain reaction	705:752	quantitative real-time polymerase chain reaction (qRT-PCR)	705:762	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	25	theme	quantitative	705:716	arg1	qRT-PCR					755:761	qRT-PCR	755:761	qRT-PCR	755:761	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	5	26	from	content	976:982	arg1	serum					987:991	serum	987:991	serum	987:991	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	1	27	theme	liver	170:174	arg1	diseases					176:183	liver diseases	170:183	liver diseases	170:183	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	0	28	theme	Gut	0:2	arg1	Modulation					15:24	Gut Microbiota Modulation	0:24	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.	0:102	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	5	29	theme	cell	929:932	arg1	proteins					949:956	intestinal epithelial cell tight junction proteins	907:956	intestinal epithelial cell tight junction proteins	907:956	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	1	30	theme	diseases	176:183	arg1	degree					160:165	the degree	156:165	the degree of liver diseases in rats	156:191	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	5	31	theme	intestinal	907:916	arg1	proteins					949:956	intestinal epithelial cell tight junction proteins	907:956	intestinal epithelial cell tight junction proteins	907:956	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	4	32	theme	polymerase	728:737	arg1	reaction					745:752	quantitative real-time polymerase chain reaction	705:752	quantitative real-time polymerase chain reaction (qRT-PCR)	705:762	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	32	theme	polymerase	728:737	arg1	qRT-PCR					755:761	qRT-PCR	755:761	qRT-PCR	755:761	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	33	theme	signaling	593:601	arg1	pathways					603:610	inflammatory signaling pathways	580:610	inflammatory signaling pathways	580:610	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	5	34	theme	gut	791:793	arg1	composition					806:816	the gut microbiota composition	787:816	the gut microbiota composition	787:816	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	4	35	theme	apoptosis	520:528	arg1	proteins					530:537	apoptosis proteins	520:537	apoptosis proteins	520:537	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	0	36	from	Diseases	86:93	arg1	Rats					98:101	Rats	98:101	Rats	98:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	1	37	theme	Aronia	104:109	arg1	AMPs					136:139	AMPs	136:139	AMPs	136:139	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	1	37	theme	Aronia	104:109	arg1	polyphenols					123:133	Aronia melanocarpa polyphenols	104:133	Aronia melanocarpa polyphenols (AMPs)	104:140	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	4	38	theme	inflammatory	580:591	arg1	pathways					603:610	inflammatory signaling pathways	580:610	inflammatory signaling pathways	580:610	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	7	39	theme	barrier	1238:1244	arg1	function					1246:1253	the intestinal barrier function	1223:1253	the intestinal barrier function	1223:1253	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	7	39	theme	barrier	1238:1244	arg1	substance					1198:1206	a bioactive substance	1186:1206	a bioactive substance	1186:1206	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	3	40	used	used	355:358	arg2	extract					318:324	a rich-polyphenol extract	300:324	a rich-polyphenol extract of A. melanocarpa (AMPs)	300:349	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	7	41	theme	diseases	1308:1315	arg1	microbiota					1276:1285	the gut microbiota	1268:1285	the gut microbiota of LPS-induced liver diseases	1268:1315	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	4	42	theme	western	633:639	arg1	analysis					646:653	western blot analysis	633:653	western blot analysis	633:653	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	6	43	theme	inflammatory	1019:1030	arg1	factors					1032:1038	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	43	theme	inflammatory	1019:1030	arg1	interleukin					1040:1050	interleukin 6	1040:1052	interleukin 6 (IL-6)	1040:1059	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	43	theme	inflammatory	1019:1030	arg1	factor					1101:1106	tumor necrosis factor α	1086:1108	tumor necrosis factor α (TNF-α)	1086:1116	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	43	theme	inflammatory	1019:1030	arg1	interleukin					1062:1072	interleukin 1β	1062:1075	interleukin 1β (IL-1β)	1062:1083	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	4	44	theme	liver	484:488	arg1	index					499:503	liver function index	484:503	liver function index	484:503	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	44	theme	liver	484:488	arg1	disease					475:481	the anti-LPS-induced liver disease	448:481	the anti-LPS-induced liver disease	448:481	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	7	45	theme	bioactive	1188:1196	arg1	function					1246:1253	the intestinal barrier function	1223:1253	the intestinal barrier function	1223:1253	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	7	45	theme	bioactive	1188:1196	arg1	substance					1198:1206	a bioactive substance	1186:1206	a bioactive substance	1186:1206	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	4	46	theme	pathways	603:610	arg1	proteins					530:537	apoptosis proteins	520:537	apoptosis proteins	520:537	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	46	theme	pathways	603:610	arg1	factors					553:559	inflammatory factors	540:559	inflammatory factors	540:559	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	46	theme	pathways	603:610	arg1	activation					566:575	activation	566:575	activation of inflammatory signaling pathways	566:610	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	5	47	theme	barrier	858:864	arg1	function					866:873	the intestinal barrier function	843:873	the intestinal barrier function	843:873	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	5	48	theme	AMPs	771:774	arg1	treatment					776:784	AMPs treatment	771:784	AMPs treatment	771:784	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	7	49	theme	LPS-induced	1290:1300	arg1	diseases					1308:1315	LPS-induced liver diseases	1290:1315	LPS-induced liver diseases	1290:1315	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	1	50	theme	melanocarpa	111:121	arg1	AMPs					136:139	AMPs	136:139	AMPs	136:139	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	1	50	theme	melanocarpa	111:121	arg1	polyphenols					123:133	Aronia melanocarpa polyphenols	104:133	Aronia melanocarpa polyphenols (AMPs)	104:140	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	0	51	theme	Aronia	46:51	arg1	melanocarpa					53:63	Aronia melanocarpa	46:63	Aronia melanocarpa of LPS-Induced Liver Diseases in Rats	46:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	7	52	theme	liver	1302:1306	arg1	diseases					1308:1315	LPS-induced liver diseases	1290:1315	LPS-induced liver diseases	1290:1315	These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.					
33688735	4	53	theme	function	490:497	arg1	index					499:503	liver function index	484:503	liver function index	484:503	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	53	theme	function	490:497	arg1	disease					475:481	the anti-LPS-induced liver disease	448:481	the anti-LPS-induced liver disease	448:481	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	5	54	theme	intestinal	847:856	arg1	function					866:873	the intestinal barrier function	843:873	the intestinal barrier function	843:873	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	5	55	theme	LPS	972:974	arg1	content					976:982	the LPS content	968:982	the LPS content in serum	968:991	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	5	56	theme	tight	934:938	arg1	proteins					949:956	intestinal epithelial cell tight junction proteins	907:956	intestinal epithelial cell tight junction proteins	907:956	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	6	57	theme	necrosis	1092:1099	arg1	factors					1032:1038	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	57	theme	necrosis	1092:1099	arg1	factor					1101:1106	tumor necrosis factor α	1086:1108	tumor necrosis factor α (TNF-α)	1086:1116	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	57	theme	necrosis	1092:1099	arg1	TNF-α					1111:1115	TNF-α	1111:1115	TNF-α	1111:1115	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	0	58	from	Rats	98:101	arg1	melanocarpa					53:63	Aronia melanocarpa	46:63	Aronia melanocarpa of LPS-Induced Liver Diseases in Rats	46:101	Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats.					
33688735	4	59	theme	rRNA	686:689	arg1	sequencing					691:700	16S rRNA sequencing	682:700	16S rRNA sequencing	682:700	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	6	60	theme	tumor	1086:1090	arg1	factors					1032:1038	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	1019:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	60	theme	tumor	1086:1090	arg1	factor					1101:1106	tumor necrosis factor α	1086:1108	tumor necrosis factor α (TNF-α)	1086:1116	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	60	theme	tumor	1086:1090	arg1	TNF-α					1111:1115	TNF-α	1111:1115	TNF-α	1111:1115	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	6	61	theme	expression	998:1007	arg1	levels					1009:1014	The expression levels	994:1014	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs	994:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	3	62	theme	rich-polyphenol	302:316	arg1	extract					318:324	a rich-polyphenol extract	300:324	a rich-polyphenol extract of A. melanocarpa (AMPs)	300:349	Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.					
33688735	5	63	theme	microbiota	795:804	arg1	composition					806:816	the gut microbiota composition	787:816	the gut microbiota composition	787:816	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	5	64	theme	junction	940:947	arg1	proteins					949:956	intestinal epithelial cell tight junction proteins	907:956	intestinal epithelial cell tight junction proteins	907:956	After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum.					
33688735	4	65	theme	anti-LPS-induced	452:467	arg1	index					499:503	liver function index	484:503	liver function index	484:503	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	4	65	theme	anti-LPS-induced	452:467	arg1	disease					475:481	the anti-LPS-induced liver disease	448:481	the anti-LPS-induced liver disease	448:481	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33688735	6	66	theme	related	1123:1129	arg1	mRNAs					1131:1135	related mRNAs	1123:1135	related mRNAs	1123:1135	The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced.					
33688735	1	67	from	degree	160:165	arg1	rats					188:191	rats	188:191	rats	188:191	Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats.					
33688735	4	68	theme	activation	566:575	arg1	expression					506:515	expression	506:515	expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways	506:610	To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR).					
33539995	3	0	theme	@	618:618	arg1	CA					619:620	@CA	618:620	@CA	618:620	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	6	1	theme	obtained	1140:1147	arg1	scaffolds					1161:1169	the obtained nanofibrous scaffolds	1136:1169	the obtained nanofibrous scaffolds	1136:1169	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	4	2	theme	@	937:937	arg1	CA					938:939	@CA	937:939	@CA	937:939	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	5	3	theme	ZnO/GO	1060:1065	arg1	CA					1067:1068	ZnO/GO@CA	1060:1068	ZnO/GO@CA	1060:1068	Moreover, the cell viability was investigated and showed a progression of 97.38 ± 3.9% for ZnO/GO@CA.					
33539995	0	4	theme	healing	86:92	arg1	applications					94:105	wound healing applications	80:105	wound healing applications	80:105	Nanofibers of cellulose acetate containing ZnO nanoparticles/graphene oxide for wound healing applications.					
33539995	4	5	theme	tremendous	745:754	arg1	variation					756:764	a tremendous variation	743:764	a tremendous variation of tensile strength	743:784	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	1	6	theme	graphene	160:167	arg1	oxide					169:173	graphene oxide	160:173	graphene oxide	160:173	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	4	7	theme	ZnO/GO	945:950	arg1	CA					952:953	ZnO/GO@CA	945:953	ZnO/GO@CA	945:953	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	1	8	theme	new	230:232	arg1	functionality					234:246	a new functionality	228:246	a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application	228:332	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	4	9	theme	tensile	769:775	arg1	strength					777:784	tensile strength	769:784	tensile strength	769:784	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	3	10	dep	651.9 nm	489:496	arg1	to					486:487	to	486:487	to	486:487	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	3	11	theme	ZnO/GO	627:632	arg1	CA					634:635	ZnO/GO@CA	627:635	ZnO/GO@CA	627:635	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	6	12	theme	cell	1118:1121	arg1	line					1123:1126	cell line	1118:1126	human fibroblasts cell line towards the obtained nanofibrous scaffolds	1100:1169	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	6	13	theme	fibers	1335:1340	arg1	morphology					1317:1326	the morphology	1313:1326	the morphology of the fibers	1313:1340	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	1	14	theme	oxide	169:173	arg1	combination					110:120	A combination	108:120	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA)	108:217	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	4	15	dep	12.87 ± 0.93	806:817	arg1	5.44 ± 0.81					791:801	5.44 ± 0.81	791:801	5.44 ± 0.81	791:801	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	5	16	theme	cell	983:986	arg1	viability					988:996	the cell viability	979:996	the cell viability	979:996	Moreover, the cell viability was investigated and showed a progression of 97.38 ± 3.9% for ZnO/GO@CA.					
33539995	3	17	theme	roughness	457:465	arg1	height					443:448	the maximum height	431:448	the maximum height of the roughness	431:465	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	1	18	with	combination	110:120	arg1	CA					215:216	CA	215:216	CA	215:216	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	1	18	with	combination	110:120	arg1	acetate					206:212	cellulose acetate	196:212	cellulose acetate (CA)	196:217	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	6	19	dep	fibroblasts	1106:1116	arg1	line					1123:1126	cell line	1118:1126	human fibroblasts cell line towards the obtained nanofibrous scaffolds	1100:1169	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	4	20	dep	CA	931:932	arg1	GO					935:936	GO	935:936	GO@CA and ZnO/GO@CA	935:953	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	3	21	dep	CA	612:613	arg1	GO					616:617	GO	616:617	GO@CA, and ZnO/GO@CA, receptively	616:648	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	1	22	theme	both	178:181	arg1	combination					110:120	A combination	108:120	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA)	108:217	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	4	23	theme	@	930:930	arg1	CA					931:932	ZnO@CA	927:932	ZnO@CA	927:932	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	1	24	theme	nanofibers	251:260	arg1	functionality					234:246	a new functionality	228:246	a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application	228:332	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	3	25	theme	powdered	528:535	arg1	phase					537:541	the powdered phase	524:541	the powdered phase	524:541	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	0	26	theme	acetate	24:30	arg1	Nanofibers					0:9	Nanofibers	0:9	Nanofibers of cellulose acetate	0:30	Nanofibers of cellulose acetate containing ZnO nanoparticles/graphene oxide for wound healing applications.					
33539995	3	27	theme	maximum	435:441	arg1	height					443:448	the maximum height	431:448	the maximum height of the roughness	431:465	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	0	28	theme	cellulose	14:22	arg1	acetate					24:30	cellulose acetate	14:30	cellulose acetate	14:30	Nanofibers of cellulose acetate containing ZnO nanoparticles/graphene oxide for wound healing applications.					
33539995	3	29	theme	@	633:633	arg1	CA					634:635	ZnO/GO@CA	627:635	ZnO/GO@CA	627:635	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	5	30	theme	%	1054:1054	arg1	progression					1028:1038	a progression	1026:1038	a progression of 97.38 ± 3.9% for ZnO/GO@CA	1026:1068	Moreover, the cell viability was investigated and showed a progression of 97.38 ± 3.9% for ZnO/GO@CA.					
33539995	2	31	theme	obtained	339:346	arg1	nanofibers					348:357	The obtained nanofibers	335:357	The obtained nanofibers	335:357	The obtained nanofibers have been investigated using XRD, FTIR, and FESEM.					
33539995	0	32	theme	ZnO	43:45	arg1	oxide					70:74	ZnO nanoparticles/graphene oxide	43:74	ZnO nanoparticles/graphene oxide for wound healing applications	43:105	Nanofibers of cellulose acetate containing ZnO nanoparticles/graphene oxide for wound healing applications.					
33539995	4	33	theme	scaffolds	702:710	arg1	properties					675:684	the mechanical properties	660:684	the mechanical properties of the obtained scaffolds	660:710	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	1	34	theme	cellulose	196:204	arg1	CA					215:216	CA	215:216	CA	215:216	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	1	34	theme	cellulose	196:204	arg1	acetate					206:212	cellulose acetate	196:212	cellulose acetate (CA)	196:217	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	1	35	theme	bio-composite	280:292	arg1	materials					294:302	bio-composite materials	280:302	bio-composite materials for wound healing application	280:332	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	5	36	theme	@	1066:1066	arg1	CA					1067:1068	ZnO/GO@CA	1060:1068	ZnO/GO@CA	1060:1068	Moreover, the cell viability was investigated and showed a progression of 97.38 ± 3.9% for ZnO/GO@CA.					
33539995	4	37	theme	obtained	693:700	arg1	scaffolds					702:710	the obtained scaffolds	689:710	the obtained scaffolds	689:710	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	4	38	theme	ZnO	927:929	arg1	CA					931:932	ZnO@CA	927:932	ZnO@CA	927:932	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	4	39	dep	68.95 ± 4.5	889:899	arg1	to					886:887	to	886:887	to	886:887	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	1	40	theme	nanostructured	125:138	arg1	oxide					145:149	nanostructured zinc oxide	125:149	nanostructured zinc oxide (ZnO)	125:155	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	1	40	theme	nanostructured	125:138	arg1	ZnO					152:154	ZnO	152:154	ZnO	152:154	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	0	41	theme	nanoparticles/graphene	47:68	arg1	oxide					70:74	ZnO nanoparticles/graphene oxide	43:74	ZnO nanoparticles/graphene oxide for wound healing applications	43:105	Nanofibers of cellulose acetate containing ZnO nanoparticles/graphene oxide for wound healing applications.					
33539995	4	42	theme	strength	777:784	arg1	variation					756:764	a tremendous variation	743:764	a tremendous variation of tensile strength	743:784	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	3	43	theme	ZnO	608:610	arg1	CA					612:613	ZnO@CA	608:613	ZnO@CA	608:613	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	6	44	theme	fibroblasts	1106:1116	arg1	adhesion					1088:1095	the adhesion	1084:1095	the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds	1084:1169	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	1	45	theme	zinc	140:143	arg1	oxide					145:149	nanostructured zinc oxide	125:149	nanostructured zinc oxide (ZnO)	125:155	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	1	45	theme	zinc	140:143	arg1	ZnO					152:154	ZnO	152:154	ZnO	152:154	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	1	46	theme	wound	308:312	arg1	healing					314:320	wound healing	308:320	wound healing application	308:332	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	3	47	theme	@	611:611	arg1	CA					612:613	ZnO@CA	608:613	ZnO@CA	608:613	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	6	48	theme	human	1100:1104	arg1	fibroblasts					1106:1116	human fibroblasts	1100:1116	human fibroblasts cell line towards the obtained nanofibrous scaffolds	1100:1169	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	1	49	theme	oxide	145:149	arg1	combination					110:120	A combination	108:120	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA)	108:217	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33539995	4	50	theme	@	951:951	arg1	CA					952:953	ZnO/GO@CA	945:953	ZnO/GO@CA	945:953	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	3	51	dep	482 nm	573:578	arg1	to					570:571	to	570:571	to	570:571	It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively.					
33539995	6	52	theme	nanofibrous	1149:1159	arg1	scaffolds					1161:1169	the obtained nanofibrous scaffolds	1136:1169	the obtained nanofibrous scaffolds	1136:1169	Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.					
33539995	4	53	theme	mechanical	664:673	arg1	properties					675:684	the mechanical properties	660:684	the mechanical properties of the obtained scaffolds	660:710	Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively.					
33539995	0	54	theme	wound	80:84	arg1	healing					86:92	wound healing	80:92	wound healing applications	80:105	Nanofibers of cellulose acetate containing ZnO nanoparticles/graphene oxide for wound healing applications.					
33539995	1	55	theme	healing	314:320	arg1	application					322:332	wound healing application	308:332	wound healing application	308:332	A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application.					
33607540	1	0	theme	increasing	373:382	arg1	interest					384:391	increasing interest	373:391	increasing interest	373:391	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	4	1	theme	membrane	902:909	arg1	bilayer					917:923	the membrane lipid bilayer	898:923	the membrane lipid bilayer portion	898:931	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	5	2	theme	membrane	1242:1249	arg1	acids					1257:1261	membrane fatty acids	1242:1261	membrane fatty acids	1242:1261	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	2	3	theme	Russian	531:537	arg1	sturgeon					539:546	the Russian sturgeon	527:546	the Russian sturgeon (Acipenser gueldenstaedti)	527:573	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	3	theme	Russian	531:537	arg1	gueldenstaedti					559:572	Acipenser gueldenstaedti	549:572	Acipenser gueldenstaedti	549:572	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	0	4	from	effect	14:19	arg1	surface					107:113	the surface	103:113	the surface of Russian sturgeon (Acipenser gueldenstaedti)	103:160	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	4	5	theme	acid	1097:1100	arg1	metabolism					1102:1111	energy supply and amino acid metabolism	1073:1111	metabolism	1102:1111	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	5	6	theme	fatty	1251:1255	arg1	acids					1257:1261	membrane fatty acids	1242:1261	membrane fatty acids	1242:1261	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	8	7	theme	antibacterial	1858:1870	arg1	material					1872:1879	safe and antibacterial material	1849:1879	safe and antibacterial material	1849:1879	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	4	8	theme	amino	1091:1095	arg1	metabolism					1102:1111	energy supply and amino acid metabolism	1073:1111	metabolism	1102:1111	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	3	9	theme	inhibitory	849:858	arg1	efficacy					860:867	an outstanding inhibitory efficacy	834:867	an outstanding inhibitory efficacy	834:867	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	6	10	theme	SEM	1407:1409	arg1	results					1411:1417	The SEM results	1403:1417	The SEM results	1403:1417	The SEM results also substantiate the damage of the structure of the bacterial membrane caused by GAC8.					
33607540	3	11	theme	chain	717:721	arg1	length					723:728	the alkyl chain length	707:728	the alkyl chain length	707:728	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	4	12	theme	lipid	911:915	arg1	bilayer					917:923	the membrane lipid bilayer	898:923	the membrane lipid bilayer portion	898:931	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	5	13	theme	sensitive	1371:1379	arg1	bacteria					1357:1364	the bacteria	1353:1364	the bacteria more sensitive to the antibacterial	1353:1400	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	0	14	theme	Russian	118:124	arg1	sturgeon					126:133	Russian sturgeon	118:133	Russian sturgeon (Acipenser gueldenstaedti)	118:160	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	0	14	theme	Russian	118:124	arg1	gueldenstaedti					146:159	Acipenser gueldenstaedti	136:159	Acipenser gueldenstaedti	136:159	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	5	15	from	alteration	1228:1237	arg1	acids					1257:1261	membrane fatty acids	1242:1261	membrane fatty acids	1242:1261	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	2	16	attach	isolated	513:520	arg1	effectiveness					591:603	the effectiveness	587:603	the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C	587:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	16	attach	isolated	513:520	arg2	fluorescens					501:511	P. fluorescens	498:511	P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C	498:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	16	attach	isolated	513:520	arg1	gueldenstaedti					559:572	Acipenser gueldenstaedti	549:572	Acipenser gueldenstaedti	549:572	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	16	attach	isolated	513:520	arg1	sturgeon					539:546	the Russian sturgeon	527:546	the Russian sturgeon (Acipenser gueldenstaedti)	527:573	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	5	17	theme	acids	1336:1340	arg1	composition					1301:1311	the composition	1297:1311	the composition of cell membrane fatty acids	1297:1340	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	4	18	theme	tricarboxylic	1037:1049	arg1	cycle					1056:1060	both tricarboxylic acid cycle	1032:1060	both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes	1032:1142	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	3	19	theme	significant	738:748	arg1	role					750:753	a significant role	736:753	a significant role	736:753	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	2	20	theme	meats	672:676	arg1	preservation					647:658	the preservation	643:658	the preservation of sturgeon meats at 4 °C	643:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	8	21	theme	material	1872:1879	arg1	kind					1841:1844	a kind	1839:1844	a kind of safe and antibacterial material	1839:1879	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	8	21	theme	material	1872:1879	arg1	insight					1719:1725	new insight	1715:1725	new insight into the mode of action of GAC8 against P. fluorescens	1715:1780	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	3	22	theme	outstanding	837:847	arg1	efficacy					860:867	an outstanding inhibitory efficacy	834:867	an outstanding inhibitory efficacy	834:867	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	8	23	theme	great	1889:1893	arg1	promise					1895:1901	a great promise	1887:1901	a great promise for application in food preservations	1887:1939	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	2	24	theme	antibacterial	430:442	arg1	effects					444:450	the antibacterial effects	426:450	the antibacterial effects	426:450	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	4	25	theme	related	1062:1068	arg1	cycle					1056:1060	both tricarboxylic acid cycle	1032:1060	both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes	1032:1142	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	4	26	theme	fluorescens	1000:1010	arg1	growth					983:988	the growth	979:988	the growth of the P. fluorescens	979:1010	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	2	27	theme	sturgeon	663:670	arg1	meats					672:676	sturgeon meats	663:676	sturgeon meats	663:676	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	8	28	theme	action	1744:1749	arg1	mode					1736:1739	the mode	1732:1739	the mode of action of GAC8 against P. fluorescens	1732:1780	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	8	29	theme	GAC8	1754:1757	arg1	action					1744:1749	action	1744:1749	action of GAC8 against P. fluorescens	1744:1780	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	0	30	theme	Antimicrobial	0:12	arg1	effect					14:19	Antimicrobial effect	0:19	Antimicrobial effect	0:19	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	1	31	theme	non-antibiotic	295:308	arg1	agents					324:329	non-antibiotic antibacterial agents	295:329	non-antibiotic antibacterial agents	295:329	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	8	32	theme	food	1922:1925	arg1	preservations					1927:1939	food preservations	1922:1939	food preservations	1922:1939	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	7	33	theme	chitosan-based	1532:1545	arg1	films					1547:1551	the edible chitosan-based films	1521:1551	the edible chitosan-based films incorporated with GAC8	1521:1574	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33607540	1	34	theme	main	200:203	arg1	fluorescens					181:191	Pseudomonas fluorescens	169:191	Pseudomonas fluorescens	169:191	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	1	34	theme	main	200:203	arg1	microorganism					205:217	the main microorganism	196:217	the main microorganism causing severe spoilage in refrigerated aquatic products	196:274	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	2	35	theme	esters	483:488	arg1	mechanisms					456:465	mechanisms	456:465	mechanisms	456:465	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	35	theme	esters	483:488	arg1	effects					444:450	the antibacterial effects	426:450	the antibacterial effects	426:450	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	1	36	theme	antibacterial	310:322	arg1	agents					324:329	non-antibiotic antibacterial agents	295:329	non-antibiotic antibacterial agents	295:329	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	8	37	theme	safe	1849:1852	arg1	material					1872:1879	safe and antibacterial material	1849:1879	safe and antibacterial material	1849:1879	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	2	38	theme	alkyl	470:474	arg1	esters					483:488	alkyl gallic esters	470:488	alkyl gallic esters	470:488	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	39	theme	gallic	476:481	arg1	esters					483:488	alkyl gallic esters	470:488	alkyl gallic esters	470:488	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	0	40	theme	sturgeon	126:133	arg1	surface					107:113	the surface	103:113	the surface of Russian sturgeon (Acipenser gueldenstaedti)	103:160	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	2	41	from	sturgeon	539:546	arg1	combination					608:618	combination	608:618	combination with chitosan films on the preservation of sturgeon meats at 4 °C	608:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	4	42	theme	oxygen	1157:1162	arg1	consumption					1164:1174	oxygen consumption	1157:1174	oxygen consumption	1157:1174	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	0	43	theme	non-antibiotic	38:51	arg1	gallates					59:66	non-antibiotic alkyl gallates	38:66	non-antibiotic alkyl gallates against Pseudomonas fluorescens	38:98	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	3	44	theme	antibacterial	774:786	arg1	activities					788:797	their antibacterial activities	768:797	their antibacterial activities	768:797	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	4	45	dep	bilayer	917:923	arg1	portion					925:931	portion	925:931	the membrane lipid bilayer portion	898:931	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	7	46	theme	antibacterial	1596:1608	arg1	efficacy					1610:1617	the enhanced antibacterial efficacy	1583:1617	the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon	1583:1673	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33607540	4	47	theme	energy	1073:1078	arg1	supply					1080:1085	energy supply and amino acid metabolism	1073:1111	supply	1080:1085	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	1	48	theme	severe	227:232	arg1	spoilage					234:241	severe spoilage	227:241	severe spoilage in refrigerated aquatic products	227:274	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	6	49	theme	structure	1455:1463	arg1	damage					1441:1446	the damage	1437:1446	the damage of the structure of the bacterial membrane caused by GAC8	1437:1504	The SEM results also substantiate the damage of the structure of the bacterial membrane caused by GAC8.					
33607540	7	50	theme	Russian	1658:1664	arg1	sturgeon					1666:1673	Russian sturgeon	1658:1673	Russian sturgeon	1658:1673	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33607540	0	51	theme	Acipenser	136:144	arg1	sturgeon					126:133	Russian sturgeon	118:133	Russian sturgeon (Acipenser gueldenstaedti)	118:160	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	0	51	theme	Acipenser	136:144	arg1	gueldenstaedti					146:159	Acipenser gueldenstaedti	136:159	Acipenser gueldenstaedti	136:159	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	2	52	from	effectiveness	591:603	arg1	combination					608:618	combination	608:618	combination with chitosan films on the preservation of sturgeon meats at 4 °C	608:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	8	53	theme	composite	1803:1811	arg1	films					1813:1817	composite films	1803:1817	composite films containing GAC8	1803:1833	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	8	54	from	application	1907:1917	arg1	preservations					1927:1939	food preservations	1922:1939	food preservations	1922:1939	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	0	55	theme	gallates	59:66	arg1	mechanism					25:33	mechanism	25:33	mechanism	25:33	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	0	55	theme	gallates	59:66	arg1	effect					14:19	Antimicrobial effect	0:19	Antimicrobial effect	0:19	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	8	56	contain	containing	1819:1828	arg1	films					1813:1817	composite films	1803:1817	composite films containing GAC8	1803:1833	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	8	56	contain	containing	1819:1828	arg2	GAC8					1830:1833	GAC8	1830:1833	GAC8	1830:1833	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	2	57	theme	chitosan	625:632	arg1	films					634:638	chitosan films	625:638	chitosan films on the preservation of sturgeon meats at 4 °C	625:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	58	from	films	634:638	arg1	preservation					647:658	the preservation	643:658	the preservation of sturgeon meats at 4 °C	643:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	1	59	from	spoilage	234:241	arg1	products					267:274	refrigerated aquatic products	246:274	refrigerated aquatic products	246:274	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	6	60	theme	membrane	1482:1489	arg1	structure					1455:1463	the structure	1451:1463	the structure of the bacterial membrane caused by GAC8	1451:1504	The SEM results also substantiate the damage of the structure of the bacterial membrane caused by GAC8.					
33607540	0	61	theme	alkyl	53:57	arg1	gallates					59:66	non-antibiotic alkyl gallates	38:66	non-antibiotic alkyl gallates against Pseudomonas fluorescens	38:98	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	3	62	theme	octyl	803:807	arg1	gallate					809:815	octyl gallate	803:815	octyl gallate (GAC8)	803:822	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	3	62	theme	octyl	803:807	arg1	GAC8					818:821	GAC8	818:821	GAC8	818:821	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	7	63	theme	edible	1525:1530	arg1	films					1547:1551	the edible chitosan-based films	1521:1551	the edible chitosan-based films incorporated with GAC8	1521:1574	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33607540	2	64	from	4 °C	681:684	arg1	preservation					647:658	the preservation	643:658	the preservation of sturgeon meats at 4 °C	643:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	5	65	theme	membrane	1321:1328	arg1	acids					1336:1340	cell membrane fatty acids	1316:1340	cell membrane fatty acids	1316:1340	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	7	66	theme	sturgeon	1666:1673	arg1	life					1650:1653	the shelf life	1640:1653	the shelf life of Russian sturgeon	1640:1673	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33607540	3	67	theme	alkyl	711:715	arg1	length					723:728	the alkyl chain length	707:728	the alkyl chain length	707:728	Our data shows that the alkyl chain length plays a significant role in eliciting their antibacterial activities and octyl gallate (GAC8) exhibited an outstanding inhibitory efficacy.					
33607540	7	68	theme	enhanced	1587:1594	arg1	efficacy					1610:1617	the enhanced antibacterial efficacy	1583:1617	the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon	1583:1673	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33607540	5	69	theme	fatty	1330:1334	arg1	acids					1336:1340	cell membrane fatty acids	1316:1340	cell membrane fatty acids	1316:1340	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	6	70	theme	bacterial	1472:1480	arg1	membrane					1482:1489	the bacterial membrane	1468:1489	the bacterial membrane caused by GAC8	1468:1504	The SEM results also substantiate the damage of the structure of the bacterial membrane caused by GAC8.					
33607540	4	71	theme	cell	1129:1132	arg1	membranes					1134:1142	cell membranes	1129:1142	cell membranes	1129:1142	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	1	72	theme	refrigerated	246:257	arg1	products					267:274	refrigerated aquatic products	246:274	refrigerated aquatic products	246:274	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	2	73	theme	Acipenser	549:557	arg1	sturgeon					539:546	the Russian sturgeon	527:546	the Russian sturgeon (Acipenser gueldenstaedti)	527:573	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	2	73	theme	Acipenser	549:557	arg1	gueldenstaedti					559:572	Acipenser gueldenstaedti	549:572	Acipenser gueldenstaedti	549:572	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	5	74	theme	cell	1316:1319	arg1	acids					1336:1340	cell membrane fatty acids	1316:1340	cell membrane fatty acids	1316:1340	Moreover, the alteration in membrane fatty acids indicated that GAC8 could disrupt the composition of cell membrane fatty acids, rendering the bacteria more sensitive to the antibacterial.					
33607540	4	75	theme	respiration	1195:1205	arg1	chain					1207:1211	the respiration chain	1191:1211	the respiration chain	1191:1211	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	8	76	theme	new	1715:1717	arg1	kind					1841:1844	a kind	1839:1844	a kind of safe and antibacterial material	1839:1879	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	8	76	theme	new	1715:1717	arg1	insight					1719:1725	new insight	1715:1725	new insight into the mode of action of GAC8 against P. fluorescens	1715:1780	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	1	77	theme	aquatic	259:265	arg1	products					267:274	refrigerated aquatic products	246:274	refrigerated aquatic products	246:274	Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest.					
33607540	4	78	theme	acid	1051:1054	arg1	cycle					1056:1060	both tricarboxylic acid cycle	1032:1060	both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes	1032:1142	GAC8 can rapidly enter into the membrane lipid bilayer portion to disorder the membrane, and further inhibit the growth of the P. fluorescens through interfering both tricarboxylic acid cycle related to energy supply and amino acid metabolism associated with cell membranes, suppressing oxygen consumption and disturbing the respiration chain.					
33607540	0	79	from	mechanism	25:33	arg1	surface					107:113	the surface	103:113	the surface of Russian sturgeon (Acipenser gueldenstaedti)	103:160	Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti).					
33607540	8	80	dep	provide	1707:1713	arg1	have					1882:1885	have	1882:1885	have a great promise for application in food preservations	1882:1939	Overall, our findings not only provide new insight into the mode of action of GAC8 against P. fluorescens but also demonstrate composite films containing GAC8, as a kind of safe and antibacterial material, have a great promise for application in food preservations.					
33607540	2	81	with	combination	608:618	arg1	films					634:638	chitosan films	625:638	chitosan films on the preservation of sturgeon meats at 4 °C	625:684	This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser gueldenstaedti), as well as the effectiveness in combination with chitosan films on the preservation of sturgeon meats at 4 °C.					
33607540	7	82	theme	shelf	1644:1648	arg1	life					1650:1653	the shelf life	1640:1653	the shelf life of Russian sturgeon	1640:1673	Additionally, the edible chitosan-based films incorporated with GAC8 showed the enhanced antibacterial efficacy to remarkably extend the shelf life of Russian sturgeon.					
33798585	3	0	with	RC	583:584	arg1	size					600:603	smallest size	591:603	smallest size (942 nm)	591:612	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	3	0	with	RC	583:584	arg1	nm					610:611	942 nm	606:611	942 nm	606:611	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	1	1	theme	cellulose	170:178	arg1	CNS					193:195	CNS	193:195	CNS	193:195	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	1	1	theme	cellulose	170:178	arg1	nanospheres					180:190	cellulose nanospheres	170:190	cellulose nanospheres (CNS)	170:196	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	6	2	located	present	911:917	arg1	precipitate					922:932	precipitate	922:932	precipitate	922:932	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	6	2	located	present	911:917	arg2	CNS					902:904	CNS	902:904	CNS	902:904	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	1	3	theme	green	257:261	arg1	material					272:279	green template material	257:279	green template material	257:279	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	1	3	theme	green	257:261	arg1	stabilizer					291:300	emulsion stabilizer	282:300	emulsion stabilizer	282:300	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	7	4	theme	CNS	1041:1043	arg1	structure					1028:1036	The structure	1024:1036	The structure of CNS	1024:1043	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	4	5	theme	°C	755:756	arg1	p-TsOH					735:740	67 wt% p-TsOH	728:740	67 wt% p-TsOH	728:740	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	4	5	theme	°C	755:756	arg1	hydrolysis					716:725	acid hydrolysis	711:725	acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h	711:761	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	7	6	theme	Fourier	1059:1065	arg1	Diffraction					1112:1122	X-ray Diffraction	1106:1122	X-ray Diffraction (XRD)	1106:1128	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	6	theme	Fourier	1059:1065	arg1	CNS					1165:1167	CNS	1165:1167	CNS	1165:1167	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	6	theme	Fourier	1059:1065	arg1	FTIR					1099:1102	FTIR	1099:1102	FTIR	1099:1102	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	6	theme	Fourier	1059:1065	arg1	Resonance					1148:1156	Nuclear Magnetic Resonance	1131:1156	Nuclear Magnetic Resonance (NMR)	1131:1162	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	6	theme	Fourier	1059:1065	arg1	Spectroscopy					1085:1096	Fourier Transfer Infrared Spectroscopy	1059:1096	Fourier Transfer Infrared Spectroscopy (FTIR)	1059:1103	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	1	7	theme	template	263:270	arg1	material					272:279	green template material	257:279	green template material	257:279	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	1	7	theme	template	263:270	arg1	stabilizer					291:300	emulsion stabilizer	282:300	emulsion stabilizer	282:300	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	0	8	theme	two-step	113:120	arg1	hydrolysis					97:106	p-toluenesulfonic hydrolysis	79:106	p-toluenesulfonic hydrolysis	79:106	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	0	8	theme	two-step	113:120	arg1	method					122:127	a two-step method	111:127	a two-step method	111:127	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	0	8	theme	two-step	113:120	arg1	pretreatment					62:73	ZnCl2·3H2O pretreatment	51:73	ZnCl2·3H2O pretreatment	51:73	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	3	9	theme	2	633:633	arg1	h					635:635	h	635:635	h	635:635	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	9	10	theme	%	1388:1388	arg1	CNS					1390:1392	1 wt% CNS	1384:1392	1 wt% CNS	1384:1392	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	9	10	theme	%	1388:1388	arg1	water/ethanol/THF					1365:1381	water/ethanol/THF	1365:1381	water/ethanol/THF	1365:1381	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	5	11	theme	average	874:880	arg1	diameter					882:889	an average diameter	871:889	an average diameter of 347 nm	871:899	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	12	theme	Electron	825:832	arg1	Microscopy					834:843	Transmission Electron Microscopy	812:843	Transmission Electron Microscopy (TEM)	812:849	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	12	theme	Electron	825:832	arg1	Scattering					794:803	Dynamic Light Scattering	780:803	Dynamic Light Scattering (DLS)	780:809	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	12	theme	Electron	825:832	arg1	TEM					846:848	TEM	846:848	TEM	846:848	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	13	theme	Transmission	812:823	arg1	Microscopy					834:843	Transmission Electron Microscopy	812:843	Transmission Electron Microscopy (TEM)	812:849	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	13	theme	Transmission	812:823	arg1	Scattering					794:803	Dynamic Light Scattering	780:803	Dynamic Light Scattering (DLS)	780:809	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	13	theme	Transmission	812:823	arg1	TEM					846:848	TEM	846:848	TEM	846:848	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	10	14	theme	7.37	1428:1431	arg1	%					1432:1432	%	1432:1432	%	1432:1432	7.37% rod-like CNC were obtained after 6 h hydrolysis.					
33798585	6	15	attach	present	911:917	arg1	precipitate					922:932	precipitate	922:932	precipitate	922:932	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	6	15	attach	present	911:917	arg2	CNS					902:904	CNS	902:904	CNS	902:904	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	10	16	theme	h	1469:1469	arg1	hydrolysis					1471:1480	6 h hydrolysis	1467:1480	6 h hydrolysis	1467:1480	7.37% rod-like CNC were obtained after 6 h hydrolysis.					
33798585	2	17	theme	two-step	339:346	arg1	method					348:353	a new two-step method	333:353	a new two-step method	333:353	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	6	18	from	present	911:917	arg1	precipitate					922:932	precipitate	922:932	precipitate	922:932	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	6	19	theme	mV	1005:1006	arg1	potential					988:996	the high Zeta potential	974:996	the high Zeta potential of -12 mV and large size	974:1021	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	9	20	dep	showed	1332:1337	arg1	dissolved					1403:1411	dissolved	1403:1411	showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O	1332:1425	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	7	21	theme	%	1212:1212	arg1	crystallinity					1178:1190	high crystallinity	1173:1190	high crystallinity (cellulose II) of 61%	1173:1212	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	21	theme	%	1212:1212	arg1	II					1203:1204	cellulose II	1193:1204	cellulose II	1193:1204	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	6	22	theme	due	967:969	arg1	centrifugation					951:964	high-speed centrifugation	940:964	high-speed centrifugation	940:964	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	8	23	theme	thermal	1278:1284	arg1	stability					1286:1294	high thermal stability	1273:1294	high thermal stability	1273:1294	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	9	24	from	re-dispersibility	1344:1360	arg1	CNS					1390:1392	1 wt% CNS	1384:1392	1 wt% CNS	1384:1392	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	9	24	from	re-dispersibility	1344:1360	arg1	water/ethanol/THF					1365:1381	water/ethanol/THF	1365:1381	water/ethanol/THF	1365:1381	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	2	25	theme	cellulose	395:403	arg1	pretreatment					362:373	first pretreatment	356:373	first pretreatment of microcrystalline cellulose (MCC)	356:409	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	10	26	theme	6	1467:1467	arg1	h					1469:1469	h	1469:1469	h	1469:1469	7.37% rod-like CNC were obtained after 6 h hydrolysis.					
33798585	12	27	theme	spherical	1624:1632	arg1	CNS					1634:1636	spherical CNS	1624:1636	spherical CNS	1624:1636	This study provided a novel, sustainable two-step method for the preparation of spherical CNS.					
33798585	2	28	theme	p-toluenesulfonic	487:503	arg1	p-TsOH					511:516	p-TsOH	511:516	p-TsOH	511:516	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	2	28	theme	p-toluenesulfonic	487:503	arg1	acid					505:508	p-toluenesulfonic acid	487:508	p-toluenesulfonic acid (p-TsOH)	487:517	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	4	29	theme	high	674:677	arg1	yield					685:689	high 61.3% yield	674:689	high 61.3% yield	674:689	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	8	30	theme	high	1273:1276	arg1	stability					1286:1294	high thermal stability	1273:1294	high thermal stability	1273:1294	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	2	31	theme	microcrystalline	378:393	arg1	cellulose					395:403	microcrystalline cellulose	378:403	microcrystalline cellulose (MCC)	378:409	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	2	31	theme	microcrystalline	378:393	arg1	MCC					406:408	MCC	406:408	MCC	406:408	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	2	32	theme	acid	437:440	arg1	hydrolysis					442:451	acid hydrolysis	437:451	acid hydrolysis of regenerated cellulose (RC)	437:481	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	1	33	theme	emulsion	282:289	arg1	material					272:279	green template material	257:279	green template material	257:279	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	1	33	theme	emulsion	282:289	arg1	stabilizer					291:300	emulsion stabilizer	282:300	emulsion stabilizer	282:300	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	7	34	theme	cellulose	1193:1201	arg1	crystallinity					1178:1190	high crystallinity	1173:1190	high crystallinity (cellulose II) of 61%	1173:1212	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	34	theme	cellulose	1193:1201	arg1	II					1203:1204	cellulose II	1193:1204	cellulose II	1193:1204	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	9	35	theme	wt	1386:1387	arg1	CNS					1390:1392	1 wt% CNS	1384:1392	1 wt% CNS	1384:1392	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	9	35	theme	wt	1386:1387	arg1	water/ethanol/THF					1365:1381	water/ethanol/THF	1365:1381	water/ethanol/THF	1365:1381	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	12	36	theme	CNS	1634:1636	arg1	preparation					1609:1619	the preparation	1605:1619	the preparation of spherical CNS	1605:1636	This study provided a novel, sustainable two-step method for the preparation of spherical CNS.					
33798585	8	37	theme	Tonset	1297:1302	arg1	°C					1310:1311	Tonset 303.3 °C	1297:1311	Tonset 303.3 °C	1297:1311	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	5	38	theme	Dynamic	780:786	arg1	Microscopy					834:843	Transmission Electron Microscopy	812:843	Transmission Electron Microscopy (TEM)	812:849	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	38	theme	Dynamic	780:786	arg1	DLS					806:808	DLS	806:808	DLS	806:808	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	38	theme	Dynamic	780:786	arg1	Scattering					794:803	Dynamic Light Scattering	780:803	Dynamic Light Scattering (DLS)	780:809	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	4	39	theme	%	683:683	arg1	yield					685:689	high 61.3% yield	674:689	high 61.3% yield	674:689	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	8	40	contain	had	1269:1271	arg1	CNS					1265:1267	CNS	1265:1267	CNS	1265:1267	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	8	40	contain	had	1269:1271	arg2	stability					1286:1294	high thermal stability	1273:1294	high thermal stability	1273:1294	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	0	41	theme	cellulose	15:23	arg1	Preparation					0:10	Preparation	0:10	Preparation of cellulose	0:23	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	8	42	theme	Gravimetric	1223:1233	arg1	TGA					1245:1247	TGA	1245:1247	TGA	1245:1247	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	8	42	theme	Gravimetric	1223:1233	arg1	Analysis					1235:1242	Thermal Gravimetric Analysis	1215:1242	Thermal Gravimetric Analysis (TGA)	1215:1248	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	6	43	theme	large	1012:1016	arg1	size					1018:1021	large size	1012:1021	large size	1012:1021	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	4	44	theme	61.3	679:682	arg1	%					683:683	%	683:683	%	683:683	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	1	45	theme	controllable	204:215	arg1	morphology					217:226	controllable morphology	204:226	controllable morphology	204:226	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	5	46	theme	Light	788:792	arg1	Microscopy					834:843	Transmission Electron Microscopy	812:843	Transmission Electron Microscopy (TEM)	812:849	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	46	theme	Light	788:792	arg1	DLS					806:808	DLS	806:808	DLS	806:808	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	46	theme	Light	788:792	arg1	Scattering					794:803	Dynamic Light Scattering	780:803	Dynamic Light Scattering (DLS)	780:809	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	8	47	dep	°C	1323:1324	arg1	°C					1310:1311	Tonset 303.3 °C	1297:1311	Tonset 303.3 °C	1297:1311	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	8	47	dep	°C	1323:1324	arg1	Tmax					1314:1317	Tmax	1314:1317	Tmax	1314:1317	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	4	48	theme	%	733:733	arg1	p-TsOH					735:740	67 wt% p-TsOH	728:740	67 wt% p-TsOH	728:740	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	4	48	theme	%	733:733	arg1	hydrolysis					716:725	acid hydrolysis	711:725	acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h	711:761	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	2	49	theme	cellulose	468:476	arg1	hydrolysis					442:451	acid hydrolysis	437:451	acid hydrolysis of regenerated cellulose (RC)	437:481	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	2	49	theme	cellulose	468:476	arg1	ZnCl2·3H2O					417:426	ZnCl2·3H2O	417:426	ZnCl2·3H2O	417:426	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	7	50	theme	high	1173:1176	arg1	crystallinity					1178:1190	high crystallinity	1173:1190	high crystallinity (cellulose II) of 61%	1173:1212	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	50	theme	high	1173:1176	arg1	II					1203:1204	cellulose II	1193:1204	cellulose II	1193:1204	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	3	51	theme	smallest	591:598	arg1	size					600:603	smallest size	591:603	smallest size (942 nm)	591:612	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	3	51	theme	smallest	591:598	arg1	nm					610:611	942 nm	606:611	942 nm	606:611	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	4	52	theme	wt	731:732	arg1	p-TsOH					735:740	67 wt% p-TsOH	728:740	67 wt% p-TsOH	728:740	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	4	52	theme	wt	731:732	arg1	hydrolysis					716:725	acid hydrolysis	711:725	acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h	711:761	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	7	53	theme	X-ray	1106:1110	arg1	Diffraction					1112:1122	X-ray Diffraction	1106:1122	X-ray Diffraction (XRD)	1106:1128	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	53	theme	X-ray	1106:1110	arg1	Spectroscopy					1085:1096	Fourier Transfer Infrared Spectroscopy	1059:1096	Fourier Transfer Infrared Spectroscopy (FTIR)	1059:1103	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	53	theme	X-ray	1106:1110	arg1	XRD					1125:1127	XRD	1125:1127	XRD	1125:1127	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	2	54	theme	regenerated	456:466	arg1	RC					479:480	RC	479:480	RC	479:480	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	2	54	theme	regenerated	456:466	arg1	cellulose					468:476	regenerated cellulose	456:476	regenerated cellulose (RC)	456:481	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	8	55	theme	Thermal	1215:1221	arg1	TGA					1245:1247	TGA	1245:1247	TGA	1245:1247	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	8	55	theme	Thermal	1215:1221	arg1	Analysis					1235:1242	Thermal Gravimetric Analysis	1215:1242	Thermal Gravimetric Analysis (TGA)	1215:1248	Thermal Gravimetric Analysis (TGA) indicated that CNS had high thermal stability (Tonset 303.3 °C, Tmax 332 °C).					
33798585	3	56	theme	MCC	554:556	arg1	shape					524:528	shape	524:528	shape	524:528	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	3	56	theme	MCC	554:556	arg1	crystallinity					537:549	crystallinity	537:549	crystallinity	537:549	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	3	56	theme	MCC	554:556	arg1	size					531:534	size	531:534	size	531:534	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	10	57	theme	rod-like	1434:1441	arg1	CNC					1443:1445	7.37% rod-like CNC	1428:1445	7.37% rod-like CNC	1428:1445	7.37% rod-like CNC were obtained after 6 h hydrolysis.					
33798585	1	58	theme	Spherical	130:138	arg1	nanocelluloses					140:153	Spherical nanocelluloses	130:153	Spherical nanocelluloses	130:153	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	0	59	theme	ZnCl2·3H2O	51:60	arg1	hydrolysis					97:106	p-toluenesulfonic hydrolysis	79:106	p-toluenesulfonic hydrolysis	79:106	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	0	59	theme	ZnCl2·3H2O	51:60	arg1	method					122:127	a two-step method	111:127	a two-step method	111:127	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	0	59	theme	ZnCl2·3H2O	51:60	arg1	pretreatment					62:73	ZnCl2·3H2O pretreatment	51:73	ZnCl2·3H2O pretreatment	51:73	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	4	60	with	CNS	665:667	arg1	yield					685:689	high 61.3% yield	674:689	high 61.3% yield	674:689	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	9	61	theme	poor	1339:1342	arg1	re-dispersibility					1344:1360	poor re-dispersibility	1339:1360	poor re-dispersibility in water/ethanol/THF, 1 wt% CNS	1339:1392	CNS showed poor re-dispersibility in water/ethanol/THF, 1 wt% CNS could be dissolved in ZnCl2·3H2O.					
33798585	7	62	theme	Nuclear	1131:1137	arg1	NMR					1159:1161	NMR	1159:1161	NMR	1159:1161	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	62	theme	Nuclear	1131:1137	arg1	Resonance					1148:1156	Nuclear Magnetic Resonance	1131:1156	Nuclear Magnetic Resonance (NMR)	1131:1162	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	62	theme	Nuclear	1131:1137	arg1	Spectroscopy					1085:1096	Fourier Transfer Infrared Spectroscopy	1059:1096	Fourier Transfer Infrared Spectroscopy (FTIR)	1059:1103	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	2	63	theme	new	335:337	arg1	method					348:353	a new two-step method	333:353	a new two-step method	333:353	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	6	64	theme	size	1018:1021	arg1	potential					988:996	the high Zeta potential	974:996	the high Zeta potential of -12 mV and large size	974:1021	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	7	65	theme	Infrared	1076:1083	arg1	Diffraction					1112:1122	X-ray Diffraction	1106:1122	X-ray Diffraction (XRD)	1106:1128	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	65	theme	Infrared	1076:1083	arg1	CNS					1165:1167	CNS	1165:1167	CNS	1165:1167	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	65	theme	Infrared	1076:1083	arg1	FTIR					1099:1102	FTIR	1099:1102	FTIR	1099:1102	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	65	theme	Infrared	1076:1083	arg1	Resonance					1148:1156	Nuclear Magnetic Resonance	1131:1156	Nuclear Magnetic Resonance (NMR)	1131:1162	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	65	theme	Infrared	1076:1083	arg1	Spectroscopy					1085:1096	Fourier Transfer Infrared Spectroscopy	1059:1096	Fourier Transfer Infrared Spectroscopy (FTIR)	1059:1103	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	2	66	dep	prepared	320:327	arg1	using					411:415	using	411:415	using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH)	411:517	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	6	67	theme	Zeta	983:986	arg1	potential					988:996	the high Zeta potential	974:996	the high Zeta potential of -12 mV and large size	974:1021	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	6	68	theme	high	978:981	arg1	potential					988:996	the high Zeta potential	974:996	the high Zeta potential of -12 mV and large size	974:1021	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	12	69	theme	novel	1566:1570	arg1	method					1594:1599	a novel, sustainable two-step method	1564:1599	a novel, sustainable two-step method for the preparation of spherical CNS	1564:1636	This study provided a novel, sustainable two-step method for the preparation of spherical CNS.					
33798585	5	70	theme	nm	898:899	arg1	diameter					882:889	an average diameter	871:889	an average diameter of 347 nm	871:899	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	4	71	theme	acid	711:714	arg1	p-TsOH					735:740	67 wt% p-TsOH	728:740	67 wt% p-TsOH	728:740	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	4	71	theme	acid	711:714	arg1	hydrolysis					716:725	acid hydrolysis	711:725	acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h	711:761	CNS with high 61.3% yield were produced after acid hydrolysis (67 wt% p-TsOH) of RC at 80 °C, 6 h.					
33798585	6	72	theme	high-speed	940:949	arg1	centrifugation					951:964	high-speed centrifugation	940:964	high-speed centrifugation	940:964	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	5	73	theme	Scattering	794:803	arg1	analysis					768:775	The analysis	764:775	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM)	764:849	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	0	74	theme	p-toluenesulfonic	79:95	arg1	hydrolysis					97:106	p-toluenesulfonic hydrolysis	79:106	p-toluenesulfonic hydrolysis	79:106	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	0	74	theme	p-toluenesulfonic	79:95	arg1	method					122:127	a two-step method	111:127	a two-step method	111:127	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	0	74	theme	p-toluenesulfonic	79:95	arg1	pretreatment					62:73	ZnCl2·3H2O pretreatment	51:73	ZnCl2·3H2O pretreatment	51:73	Preparation of cellulose nanospheres via combining ZnCl2·3H2O pretreatment and p-toluenesulfonic hydrolysis as a two-step method.					
33798585	1	75	contain	have	199:202	arg2	morphology					217:226	controllable morphology	204:226	controllable morphology	204:226	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	1	75	contain	have	199:202	arg1	nanocelluloses					140:153	Spherical nanocelluloses	130:153	Spherical nanocelluloses	130:153	Spherical nanocelluloses, also known as cellulose nanospheres (CNS), have controllable morphology and have shown advantages as green template material, emulsion stabilizer.					
33798585	7	76	theme	Magnetic	1139:1146	arg1	NMR					1159:1161	NMR	1159:1161	NMR	1159:1161	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	76	theme	Magnetic	1139:1146	arg1	Resonance					1148:1156	Nuclear Magnetic Resonance	1131:1156	Nuclear Magnetic Resonance (NMR)	1131:1162	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	76	theme	Magnetic	1139:1146	arg1	Spectroscopy					1085:1096	Fourier Transfer Infrared Spectroscopy	1059:1096	Fourier Transfer Infrared Spectroscopy (FTIR)	1059:1103	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	6	77	from	precipitate	922:932	arg1	present					911:917	present	911:917	present	911:917	CNS were present in precipitate after high-speed centrifugation, due to the high Zeta potential of -12 mV and large size.					
33798585	7	78	theme	Transfer	1067:1074	arg1	Diffraction					1112:1122	X-ray Diffraction	1106:1122	X-ray Diffraction (XRD)	1106:1128	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	78	theme	Transfer	1067:1074	arg1	CNS					1165:1167	CNS	1165:1167	CNS	1165:1167	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	78	theme	Transfer	1067:1074	arg1	FTIR					1099:1102	FTIR	1099:1102	FTIR	1099:1102	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	78	theme	Transfer	1067:1074	arg1	Resonance					1148:1156	Nuclear Magnetic Resonance	1131:1156	Nuclear Magnetic Resonance (NMR)	1131:1162	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	7	78	theme	Transfer	1067:1074	arg1	Spectroscopy					1085:1096	Fourier Transfer Infrared Spectroscopy	1059:1096	Fourier Transfer Infrared Spectroscopy (FTIR)	1059:1103	The structure of CNS was tested by Fourier Transfer Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), CNS had high crystallinity (cellulose II) of 61%.					
33798585	5	79	contain	had	867:869	arg2	diameter					882:889	an average diameter	871:889	an average diameter of 347 nm	871:899	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	5	79	contain	had	867:869	arg1	CNS					863:865	CNS	863:865	CNS	863:865	The analysis of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) showed that CNS had an average diameter of 347 nm.					
33798585	12	80	dep	novel	1566:1570	arg1	two-step					1585:1592	two-step	1585:1592	two-step	1585:1592	This study provided a novel, sustainable two-step method for the preparation of spherical CNS.					
33798585	3	81	theme	h	635:635	arg1	pretreatment					637:648	2 h pretreatment	633:648	2 h pretreatment	633:648	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	3	82	theme	nubbly	576:581	arg1	RC					583:584	nubbly RC	576:584	nubbly RC with smallest size (942 nm)	576:612	The shape, size, crystallinity of MCC were changed, and nubbly RC with smallest size (942 nm) was obtained after 2 h pretreatment by ZnCl2·3H2O.					
33798585	2	83	theme	first	356:360	arg1	pretreatment					362:373	first pretreatment	356:373	first pretreatment of microcrystalline cellulose (MCC)	356:409	Herein, CNS were prepared via a new two-step method, first pretreatment of microcrystalline cellulose (MCC) using ZnCl2·3H2O and then acid hydrolysis of regenerated cellulose (RC) via p-toluenesulfonic acid (p-TsOH).					
33798585	10	84	theme	%	1432:1432	arg1	CNC					1443:1445	7.37% rod-like CNC	1428:1445	7.37% rod-like CNC	1428:1445	7.37% rod-like CNC were obtained after 6 h hydrolysis.					
33357894	2	0	theme	applications	330:341	arg1	applications					330:341	applications	330:341	applications	330:341	This technique is used to produce porous membranes for a large variety of applications.					
33357894	2	0	theme	applications	330:341	arg1	variety					319:325	a large variety	311:325	a large variety of applications	311:341	This technique is used to produce porous membranes for a large variety of applications.					
33357894	8	1	theme	maximum	969:975	arg1	value					977:981	The maximum value	965:981	The maximum value of PWF	965:988	The maximum value of PWF was obtained for membranes prepared using a PE support.					
33357894	5	2	from	effect	685:690	arg1	PWF					764:766	PWF	764:766	PWF	764:766	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	5	2	from	effect	685:690	arg1	porosity					751:758	porosity	751:758	porosity	751:758	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	5	2	from	effect	685:690	arg1	size					724:727	the mean pore size	710:727	the mean pore size	710:727	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	5	2	from	effect	685:690	arg1	angle					744:748	water contact angle	730:748	water contact angle	730:748	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	7	3	from	increase	897:904	arg1	hydrophilicity					922:935	hydrophilicity	922:935	hydrophilicity	922:935	In contrast, there was an increase in porosity and hydrophilicity with increasing Ac/W ratio.					
33357894	7	3	from	increase	897:904	arg1	porosity					909:916	porosity	909:916	porosity	909:916	In contrast, there was an increase in porosity and hydrophilicity with increasing Ac/W ratio.					
33357894	4	4	theme	precipitation	601:613	arg1	PBC					633:635	PBC	633:635	PBC	633:635	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	4	theme	precipitation	601:613	arg1	composition					620:630	the precipitation bath composition	597:630	the precipitation bath composition (PBC)	597:636	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	9	5	contain	had	1091:1093	arg2	effects					1107:1113	significant effects	1095:1113	significant effects	1095:1113	ANOVA showed that most, but not all, factors had significant effects on the parameters measured.					
33357894	9	5	contain	had	1091:1093	arg1	factors					1083:1089	most, but not all, factors	1064:1089	most, but not all, factors	1064:1089	ANOVA showed that most, but not all, factors had significant effects on the parameters measured.					
33357894	2	6	used	used	274:277	arg2	technique					261:269	This technique	256:269	This technique	256:269	This technique is used to produce porous membranes for a large variety of applications.					
33357894	4	7	theme	bath	615:618	arg1	PBC					633:635	PBC	633:635	PBC	633:635	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	7	theme	bath	615:618	arg1	composition					620:630	the precipitation bath composition	597:630	the precipitation bath composition (PBC)	597:636	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	8	from	ratio	588:592	arg1	material					650:657	support material	642:657	support material (glass/polyethylene)	642:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	8	from	ratio	588:592	arg1	PBC					633:635	PBC	633:635	PBC	633:635	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	8	from	ratio	588:592	arg1	glass/polyethylene					660:677	glass/polyethylene	660:677	glass/polyethylene	660:677	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	8	from	ratio	588:592	arg1	composition					620:630	the precipitation bath composition	597:630	the precipitation bath composition (PBC)	597:636	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	9	from	ratio	534:538	arg1	solution					555:562	the casting solution	543:562	the casting solution	543:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	1	10	theme	ultrafiltration	136:150	arg1	membranes					152:160	Cellulose acetate (CA) ultrafiltration membranes	113:160	Cellulose acetate (CA) ultrafiltration membranes	113:160	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	0	11	theme	experiments	94:104	arg1	design					84:89	the design	80:89	the design of experiments (DOE)	80:110	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	0	12	theme	produced	29:36	arg1	membranes					56:64	produced cellulose acetate membranes	29:64	produced cellulose acetate membranes	29:64	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	4	13	theme	casting	547:553	arg1	solution					555:562	the casting solution	543:562	the casting solution	543:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	3	14	theme	membrane	410:417	arg1	PWF					436:438	PWF	436:438	PWF	436:438	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	3	14	theme	membrane	410:417	arg1	flux					430:433	membrane pure water flux	410:433	membrane pure water flux (PWF)	410:439	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	6	15	theme	pore	847:850	arg1	size					852:855	mean pore size	842:855	mean pore size	842:855	The increase in the CA/NMP ratio reduced mean pore size and porosity.					
33357894	4	16	theme	support	642:648	arg1	material					650:657	support material	642:657	support material (glass/polyethylene)	642:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	16	theme	support	642:648	arg1	glass/polyethylene					660:677	glass/polyethylene	660:677	glass/polyethylene	660:677	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	8	17	theme	PE	1034:1035	arg1	support					1037:1043	a PE support	1032:1043	a PE support	1032:1043	The maximum value of PWF was obtained for membranes prepared using a PE support.					
33357894	4	18	theme	CA/NMP	502:507	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	18	theme	CA/NMP	502:507	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	7	19	theme	Ac/W	953:956	arg1	ratio					958:962	Ac/W ratio	953:962	Ac/W ratio	953:962	In contrast, there was an increase in porosity and hydrophilicity with increasing Ac/W ratio.					
33357894	9	20	theme	most	1064:1067	arg1	factors					1083:1089	most, but not all, factors	1064:1089	most, but not all, factors	1064:1089	ANOVA showed that most, but not all, factors had significant effects on the parameters measured.					
33357894	2	21	theme	porous	290:295	arg1	membranes					297:305	porous membranes	290:305	porous membranes	290:305	This technique is used to produce porous membranes for a large variety of applications.					
33357894	0	22	theme	cellulose	38:46	arg1	membranes					56:64	produced cellulose acetate membranes	29:64	produced cellulose acetate membranes	29:64	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	5	23	theme	water	730:734	arg1	angle					744:748	water contact angle	730:748	water contact angle	730:748	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	6	24	theme	mean	842:845	arg1	size					852:855	mean pore size	842:855	mean pore size	842:855	The increase in the CA/NMP ratio reduced mean pore size and porosity.					
33357894	4	25	theme	Ac	574:575	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	25	theme	Ac	574:575	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	0	26	theme	water	15:19	arg1	flux					21:24	water flux	15:24	water flux	15:24	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	4	27	theme	N-methyl-2-pyrrolidone	510:531	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	27	theme	N-methyl-2-pyrrolidone	510:531	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	5	28	theme	mean	714:717	arg1	size					724:727	the mean pore size	710:727	the mean pore size	710:727	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	3	29	theme	skin	378:381	arg1	formation					357:365	the formation	353:365	the formation of a dense skin during the process	353:400	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	4	30	theme	/water	577:582	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	30	theme	/water	577:582	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	3	31	theme	pure	419:422	arg1	PWF					436:438	PWF	436:438	PWF	436:438	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	3	31	theme	pure	419:422	arg1	flux					430:433	membrane pure water flux	410:433	membrane pure water flux (PWF)	410:439	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	4	32	theme	W	585:585	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	32	theme	W	585:585	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	6	33	theme	CA/NMP	821:826	arg1	ratio					828:832	the CA/NMP ratio	817:832	the CA/NMP ratio	817:832	The increase in the CA/NMP ratio reduced mean pore size and porosity.					
33357894	9	34	theme	all	1078:1080	arg1	factors					1083:1089	most, but not all, factors	1064:1089	most, but not all, factors	1064:1089	ANOVA showed that most, but not all, factors had significant effects on the parameters measured.					
33357894	4	35	theme	acetone	565:571	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	35	theme	acetone	565:571	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	5	36	theme	contact	736:742	arg1	angle					744:748	water contact angle	730:748	water contact angle	730:748	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	3	37	theme	water	424:428	arg1	PWF					436:438	PWF	436:438	PWF	436:438	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	3	37	theme	water	424:428	arg1	flux					430:433	membrane pure water flux	410:433	membrane pure water flux (PWF)	410:439	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	0	38	theme	acetate	48:54	arg1	membranes					56:64	produced cellulose acetate membranes	29:64	produced cellulose acetate membranes	29:64	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	5	39	theme	factor	700:705	arg1	effect					685:690	The effect	681:690	The effect of each factor on the mean pore size, water contact angle, porosity and PWF	681:766	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	8	40	theme	PWF	986:988	arg1	value					977:981	The maximum value	965:981	The maximum value of PWF	965:988	The maximum value of PWF was obtained for membranes prepared using a PE support.					
33357894	5	41	theme	Taguchi	785:791	arg1	design					793:798	Taguchi design	785:798	Taguchi design	785:798	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
33357894	3	42	theme	dense	372:376	arg1	skin					378:381	a dense skin	370:381	a dense skin	370:381	However, the formation of a dense skin during the process reduces membrane pure water flux (PWF).					
33357894	6	43	from	increase	805:812	arg1	ratio					828:832	the CA/NMP ratio	817:832	the CA/NMP ratio	817:832	The increase in the CA/NMP ratio reduced mean pore size and porosity.					
33357894	2	44	theme	large	313:317	arg1	applications					330:341	applications	330:341	applications	330:341	This technique is used to produce porous membranes for a large variety of applications.					
33357894	2	44	theme	large	313:317	arg1	variety					319:325	a large variety	311:325	a large variety of applications	311:341	This technique is used to produce porous membranes for a large variety of applications.					
33357894	4	45	dep	overcome	445:452	arg1	ratio					534:538	CA/NMP (N-methyl-2-pyrrolidone) ratio	502:538	CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution	502:562	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	4	45	dep	overcome	445:452	arg1	ratio					588:592	acetone (Ac)/water (W) ratio	565:592	acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene)	565:678	To overcome this issue, three parameters were investigated: CA/NMP (N-methyl-2-pyrrolidone) ratio in the casting solution, acetone (Ac)/water (W) ratio in the precipitation bath composition (PBC) and support material (glass/polyethylene).					
33357894	1	46	theme	induced	211:217	arg1	separation					225:234	induced phase separation	211:234	the non-solvent induced phase separation (NIPS) methodology	195:253	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	46	theme	induced	211:217	arg1	NIPS					237:240	NIPS	237:240	NIPS	237:240	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	9	47	theme	significant	1095:1105	arg1	effects					1107:1113	significant effects	1095:1113	significant effects	1095:1113	ANOVA showed that most, but not all, factors had significant effects on the parameters measured.					
33357894	1	48	theme	Cellulose	113:121	arg1	CA					132:133	CA	132:133	CA	132:133	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	48	theme	Cellulose	113:121	arg1	acetate					123:129	Cellulose acetate	113:129	Cellulose acetate (CA) ultrafiltration membranes	113:160	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	49	theme	phase	219:223	arg1	separation					225:234	induced phase separation	211:234	the non-solvent induced phase separation (NIPS) methodology	195:253	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	49	theme	phase	219:223	arg1	NIPS					237:240	NIPS	237:240	NIPS	237:240	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	50	theme	non-solvent	199:209	arg1	methodology					243:253	the non-solvent induced phase separation (NIPS) methodology	195:253	the non-solvent induced phase separation (NIPS) methodology	195:253	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	51	theme	acetate	123:129	arg1	membranes					152:160	Cellulose acetate (CA) ultrafiltration membranes	113:160	Cellulose acetate (CA) ultrafiltration membranes	113:160	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	1	52	theme	separation	225:234	arg1	methodology					243:253	the non-solvent induced phase separation (NIPS) methodology	195:253	the non-solvent induced phase separation (NIPS) methodology	195:253	Cellulose acetate (CA) ultrafiltration membranes were successfully prepared using the non-solvent induced phase separation (NIPS) methodology.					
33357894	0	53	theme	membranes	56:64	arg1	Morphology					0:9	Morphology	0:9	Morphology	0:9	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	0	53	theme	membranes	56:64	arg1	flux					21:24	water flux	15:24	water flux	15:24	Morphology and water flux of produced cellulose acetate membranes reinforced by the design of experiments (DOE).					
33357894	5	54	theme	pore	719:722	arg1	size					724:727	the mean pore size	710:727	the mean pore size	710:727	The effect of each factor on the mean pore size, water contact angle, porosity and PWF was supported by Taguchi design.					
32174148	12	0	theme	expression	2231:2240	arg1	profile					2242:2248	their gene expression profile	2220:2248	their gene expression profile	2220:2248	In addition, we demonstrate that our HAL, when used as a carrier system for co-transplanted SCs, changed their gene expression profile and deteriorated the pro-regenerative milieu within the nerve guides.					
32174148	4	1	theme	sciatic	961:967	arg1	reconstruction					979:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	0	2	theme	Critical	157:164	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	1	3	theme	nerve	417:421	arg1	CNG					430:432	CNG	430:432	CNG	430:432	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	3	theme	nerve	417:421	arg1	graft					423:427	a composite chitosan-based nerve graft	390:427	a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model	390:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	2	4	theme	guides	542:547	arg1	performance					506:516	the performance	502:516	the performance of our artificial nerve guides	502:547	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	4	5	theme	gap	975:977	arg1	reconstruction					979:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	0	6	theme	Length	170:175	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	2	7	theme	molecular	585:593	arg1	support					595:601	additional structural and molecular support	559:601	additional structural and molecular support	559:601	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	0	8	theme	Peripheral	185:194	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	10	9	theme	factors	1981:1987	arg1	expression					1930:1939	gene expression	1925:1939	gene expression of regeneration-supporting neurotrophic factors	1925:1987	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	7	10	theme	segments	1395:1402	arg1	cross-sections					1371:1384	Semi-thin cross-sections	1361:1384	Semi-thin cross-sections of nerve segments distal to the grafts	1361:1423	Semi-thin cross-sections of nerve segments distal to the grafts were evaluated histomorphometrically.					
32174148	2	11	theme	structural	570:579	arg1	support					595:601	additional structural and molecular support	559:601	additional structural and molecular support	559:601	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	3	12	theme	film	714:717	arg1	s					726:726	an inserted longitudinal chitosan film (CNG[F]s)	680:727	an inserted longitudinal chitosan film (CNG[F]s)	680:727	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	10	13	theme	in	1750:1751	arg1	studies					1759:1765	complementary in vitro studies	1736:1765	complementary in vitro studies	1736:1765	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	3	14	theme	longitudinal	692:703	arg1	s					726:726	an inserted longitudinal chitosan film (CNG[F]s)	680:727	an inserted longitudinal chitosan film (CNG[F]s)	680:727	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	0	15	theme	Carrier	98:104	arg1	System					106:111	Carrier System	98:111	Carrier System	98:111	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	9	16	theme	nerve	1667:1671	arg1	regeneration					1673:1684	peripheral nerve regeneration	1656:1684	peripheral nerve regeneration	1656:1684	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	4	17	theme	regeneration	1045:1056	arg1	parameters					1058:1067	axonal and functional regeneration parameters	1023:1067	axonal and functional regeneration parameters	1023:1067	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	1	18	theme	co-transplanted	361:375	arg1	cells					377:381	co-transplanted cells	361:381	co-transplanted cells	361:381	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	2	19	theme	artificial	525:534	arg1	guides					542:547	our artificial nerve guides	521:547	our artificial nerve guides	521:547	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	3	20	theme	CNG[F	720:724	arg1	s					726:726	an inserted longitudinal chitosan film (CNG[F]s)	680:727	an inserted longitudinal chitosan film (CNG[F]s)	680:727	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	0	21	theme	Engineered	129:138	arg1	Cells					148:152	Genetically Engineered Schwann Cells	117:152	Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats	117:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	9	22	theme	HAL	1620:1622	arg1	effect					1610:1615	no regeneration-supportive effect	1583:1615	no regeneration-supportive effect of HAL alone	1583:1628	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	9	22	theme	HAL	1620:1622	arg1	impairment					1642:1651	even an impairment	1634:1651	even an impairment of peripheral nerve regeneration	1634:1684	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	3	23	theme	cell-free	735:743	arg1	HAL					745:747	cell-free HAL	735:747	cell-free HAL	735:747	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	1	24	theme	composite	392:400	arg1	CNG					430:432	CNG	430:432	CNG	430:432	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	24	theme	composite	392:400	arg1	graft					423:427	a composite chitosan-based nerve graft	390:427	a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model	390:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	11	25	from	HAL	2015:2017	arg1	formulation					2034:2044	its current formulation	2022:2044	its current formulation	2022:2044	In conclusion, cell-free HAL in its current formulation did not qualify for optimizing regeneration outcome through CNG[F]s.					
32174148	4	26	theme	autologous	1092:1101	arg1	graft					1109:1113	autologous nerve graft	1092:1113	the gold standard autologous nerve graft (ANG) repair	1074:1126	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	4	26	theme	autologous	1092:1101	arg1	ANG					1116:1118	ANG	1116:1118	ANG	1116:1118	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	0	27	theme	Novel	37:41	arg1	Hydrogel					67:74	a Novel Hyaluronic Acid-Laminin Hydrogel	35:74	a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System	35:111	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	4	28	theme	graft	1109:1113	arg1	repair					1121:1126	the gold standard autologous nerve graft (ANG) repair	1074:1126	the gold standard autologous nerve graft (ANG) repair	1074:1126	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	0	29	theme	Acid-Laminin	54:65	arg1	Hydrogel					67:74	a Novel Hyaluronic Acid-Laminin Hydrogel	35:74	a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System	35:111	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	3	30	theme	Schwann	804:810	arg1	cells					812:816	naïve Schwann cells	798:816	naïve Schwann cells (SCs)	798:822	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	3	30	theme	Schwann	804:810	arg1	SCs					819:821	SCs	819:821	SCs	819:821	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	2	31	theme	regrowing	606:614	arg1	axons					616:620	regrowing axons	606:620	regrowing axons	606:620	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	6	32	theme	limb	1285:1288	arg1	muscles					1297:1303	lower limb target muscles	1279:1303	lower limb target muscles	1279:1303	Upon explantation after 120 days, lower limb target muscles were harvested for calculation of muscle-weight ratios.					
32174148	1	33	theme	luminal	323:329	arg1	filler					331:336	luminal filler	323:336	luminal filler	323:336	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	3	34	theme	fibroblast	827:836	arg1	factor					845:850	fibroblast growth factor	827:850	fibroblast growth factor	827:850	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	4	35	theme	immediate	945:953	arg1	reconstruction					979:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	8	36	theme	recovery	1481:1488	arg1	days					1473:1476	120 days	1469:1476	120 days of recovery	1469:1488	After 120 days of recovery, only ANG treatment led to full motor recovery.					
32174148	12	37	theme	carrier	2172:2178	arg1	system					2180:2185	a carrier system	2170:2185	a carrier system for co-transplanted SCs	2170:2209	In addition, we demonstrate that our HAL, when used as a carrier system for co-transplanted SCs, changed their gene expression profile and deteriorated the pro-regenerative milieu within the nerve guides.					
32174148	0	38	theme	In	0:1	arg1	Evaluation					21:30	In Vivo and In Vitro Evaluation	0:30	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.	0:215	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	3	39	theme	Schwann	869:875	arg1	FGF2-SCs					884:891	FGF2-SCs	884:891	FGF2-SCs	884:891	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	3	39	theme	Schwann	869:875	arg1	cells					877:881	2-overexpressing Schwann cells	852:881	2-overexpressing Schwann cells (FGF2-SCs)	852:892	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	4	40	theme	mm	958:959	arg1	reconstruction					979:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	8	41	theme	only	1491:1494	arg1	treatment					1500:1508	only ANG treatment	1491:1508	only ANG treatment	1491:1508	After 120 days of recovery, only ANG treatment led to full motor recovery.					
32174148	4	42	theme	female	924:929	arg1	rats					937:940	female Lewis rats	924:940	female Lewis rats	924:940	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	10	43	theme	negative	1822:1829	arg1	result					1831:1836	this unexpected negative result	1806:1836	this unexpected negative result	1806:1836	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	6	44	theme	muscle-weight	1339:1351	arg1	ratios					1353:1358	muscle-weight ratios	1339:1358	muscle-weight ratios	1339:1358	Upon explantation after 120 days, lower limb target muscles were harvested for calculation of muscle-weight ratios.					
32174148	1	45	theme	novel	275:279	arg1	HAL					315:317	HAL	315:317	HAL	315:317	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	45	theme	novel	275:279	arg1	hydrogel					305:312	a novel hyaluronic acid-laminin hydrogel	273:312	a novel hyaluronic acid-laminin hydrogel (HAL)	273:318	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	0	46	from	Graft	202:206	arg1	Rats					211:214	Rats	211:214	Rats	211:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	1	47	theme	nerve	453:457	arg1	model					466:470	a rat critical nerve defect model	438:470	a rat critical nerve defect model	438:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	48	theme	acid-laminin	292:303	arg1	HAL					315:317	HAL	315:317	HAL	315:317	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	48	theme	acid-laminin	292:303	arg1	hydrogel					305:312	a novel hyaluronic acid-laminin hydrogel	273:312	a novel hyaluronic acid-laminin hydrogel (HAL)	273:318	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	9	49	theme	regeneration	1552:1563	arg1	outcomes					1565:1572	regeneration outcomes	1552:1572	regeneration outcomes	1552:1572	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	8	50	theme	motor	1522:1526	arg1	recovery					1528:1535	full motor recovery	1517:1535	full motor recovery	1517:1535	After 120 days of recovery, only ANG treatment led to full motor recovery.					
32174148	3	51	theme	hollow	633:638	arg1	CNGs					640:643	hollow CNGs	633:643	hollow CNGs	633:643	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	9	52	theme	regeneration-supportive	1586:1608	arg1	effect					1610:1615	no regeneration-supportive effect	1583:1615	no regeneration-supportive effect of HAL alone	1583:1628	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	4	53	theme	axonal	1023:1028	arg1	parameters					1058:1067	axonal and functional regeneration parameters	1023:1067	axonal and functional regeneration parameters	1023:1067	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	0	54	dep	In	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	4	55	theme	nerve	969:973	arg1	reconstruction					979:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	immediate 15 mm sciatic nerve gap reconstruction	945:992	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	3	56	theme	nerve	662:666	arg1	guides					668:673	two-chambered nerve guides	648:673	two-chambered nerve guides	648:673	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	0	57	theme	Gap	166:168	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	1	58	theme	current	224:230	arg1	study					232:236	the current study	220:236	the current study	220:236	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	59	from	graft	423:427	arg1	model					466:470	a rat critical nerve defect model	438:470	a rat critical nerve defect model	438:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	0	60	from	Cells	148:152	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	0	61	theme	Tubular	177:183	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	11	62	theme	cell-free	2005:2013	arg1	HAL					2015:2017	cell-free HAL	2005:2017	cell-free HAL in its current formulation	2005:2044	In conclusion, cell-free HAL in its current formulation did not qualify for optimizing regeneration outcome through CNG[F]s.					
32174148	7	63	theme	distal	1404:1409	arg1	segments					1395:1402	nerve segments	1389:1402	nerve segments distal to the grafts	1389:1423	Semi-thin cross-sections of nerve segments distal to the grafts were evaluated histomorphometrically.					
32174148	2	64	theme	nerve	536:540	arg1	guides					542:547	our artificial nerve guides	521:547	our artificial nerve guides	521:547	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	3	65	theme	inserted	683:690	arg1	s					726:726	an inserted longitudinal chitosan film (CNG[F]s)	680:727	an inserted longitudinal chitosan film (CNG[F]s)	680:727	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	0	66	theme	Nerve	196:200	arg1	Graft					202:206	Critical Gap Length Tubular Peripheral Nerve Graft	157:206	Critical Gap Length Tubular Peripheral Nerve Graft in Rats	157:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	7	67	theme	nerve	1389:1393	arg1	segments					1395:1402	nerve segments	1389:1402	nerve segments distal to the grafts	1389:1423	Semi-thin cross-sections of nerve segments distal to the grafts were evaluated histomorphometrically.					
32174148	7	68	theme	Semi-thin	1361:1369	arg1	cross-sections					1371:1384	Semi-thin cross-sections	1361:1384	Semi-thin cross-sections of nerve segments distal to the grafts	1361:1423	Semi-thin cross-sections of nerve segments distal to the grafts were evaluated histomorphometrically.					
32174148	4	69	theme	standard	1083:1090	arg1	repair					1121:1126	the gold standard autologous nerve graft (ANG) repair	1074:1126	the gold standard autologous nerve graft (ANG) repair	1074:1126	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	10	70	theme	regeneration-supporting	1944:1966	arg1	factors					1981:1987	regeneration-supporting neurotrophic factors	1944:1987	regeneration-supporting neurotrophic factors	1944:1987	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	11	71	theme	current	2026:2032	arg1	formulation					2034:2044	its current formulation	2022:2044	its current formulation	2022:2044	In conclusion, cell-free HAL in its current formulation did not qualify for optimizing regeneration outcome through CNG[F]s.					
32174148	5	72	theme	Motor	1129:1133	arg1	recovery					1135:1142	Motor recovery	1129:1142	Motor recovery	1129:1142	Motor recovery was surveyed by means of electrodiagnostic measurements at 60, 90, and 120 days post-reconstruction.					
32174148	2	73	theme	additional	559:568	arg1	support					595:601	additional structural and molecular support	559:601	additional structural and molecular support	559:601	The HAL was meant to improve the performance of our artificial nerve guides by giving additional structural and molecular support to regrowing axons.					
32174148	4	74	theme	functional	1034:1043	arg1	parameters					1058:1067	axonal and functional regeneration parameters	1023:1067	axonal and functional regeneration parameters	1023:1067	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	1	75	theme	rat	440:442	arg1	model					466:470	a rat critical nerve defect model	438:470	a rat critical nerve defect model	438:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	10	76	theme	complementary	1736:1748	arg1	studies					1759:1765	complementary in vitro studies	1736:1765	complementary in vitro studies	1736:1765	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	9	77	theme	peripheral	1656:1665	arg1	regeneration					1673:1684	peripheral nerve regeneration	1656:1684	peripheral nerve regeneration	1656:1684	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	3	78	theme	chitosan	705:712	arg1	s					726:726	an inserted longitudinal chitosan film (CNG[F]s)	680:727	an inserted longitudinal chitosan film (CNG[F]s)	680:727	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	9	79	theme	regeneration	1673:1684	arg1	effect					1610:1615	no regeneration-supportive effect	1583:1615	no regeneration-supportive effect of HAL alone	1583:1628	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	9	79	theme	regeneration	1673:1684	arg1	impairment					1642:1651	even an impairment	1634:1651	even an impairment of peripheral nerve regeneration	1634:1684	Surprisingly, regeneration outcomes revealed no regeneration-supportive effect of HAL alone and even an impairment of peripheral nerve regeneration when combined with SCs and FGF2-SCs.					
32174148	0	80	theme	Luminal	79:85	arg1	Filler					87:92	Luminal Filler	79:92	Luminal Filler	79:92	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	0	81	theme	Schwann	140:146	arg1	Cells					148:152	Genetically Engineered Schwann Cells	117:152	Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats	117:214	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	10	82	theme	neurotrophic	1968:1979	arg1	factors					1981:1987	regeneration-supporting neurotrophic factors	1944:1987	regeneration-supporting neurotrophic factors	1944:1987	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	3	83	theme	naïve	798:802	arg1	cells					812:816	naïve Schwann cells	798:816	naïve Schwann cells (SCs)	798:822	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	3	83	theme	naïve	798:802	arg1	SCs					819:821	SCs	819:821	SCs	819:821	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	11	84	theme	regeneration	2077:2088	arg1	outcome					2090:2096	regeneration outcome	2077:2096	regeneration outcome	2077:2096	In conclusion, cell-free HAL in its current formulation did not qualify for optimizing regeneration outcome through CNG[F]s.					
32174148	12	85	theme	nerve	2306:2310	arg1	guides					2312:2317	the nerve guides	2302:2317	the nerve guides	2302:2317	In addition, we demonstrate that our HAL, when used as a carrier system for co-transplanted SCs, changed their gene expression profile and deteriorated the pro-regenerative milieu within the nerve guides.					
32174148	4	86	theme	nerve	1103:1107	arg1	graft					1109:1113	autologous nerve graft	1092:1113	the gold standard autologous nerve graft (ANG) repair	1074:1126	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	4	86	theme	nerve	1103:1107	arg1	ANG					1116:1118	ANG	1116:1118	ANG	1116:1118	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	10	87	dep	in	1750:1751	arg1	vitro					1753:1757	vitro	1753:1757	vitro	1753:1757	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	0	88	theme	Hyaluronic	43:52	arg1	Hydrogel					67:74	a Novel Hyaluronic Acid-Laminin Hydrogel	35:74	a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System	35:111	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	1	89	theme	chitosan-based	402:415	arg1	CNG					430:432	CNG	430:432	CNG	430:432	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	89	theme	chitosan-based	402:415	arg1	graft					423:427	a composite chitosan-based nerve graft	390:427	a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model	390:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	0	90	theme	Hydrogel	67:74	arg1	Evaluation					21:30	In Vivo and In Vitro Evaluation	0:30	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.	0:215	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	12	91	theme	pro-regenerative	2271:2286	arg1	milieu					2288:2293	the pro-regenerative milieu	2267:2293	the pro-regenerative milieu within the nerve guides	2267:2317	In addition, we demonstrate that our HAL, when used as a carrier system for co-transplanted SCs, changed their gene expression profile and deteriorated the pro-regenerative milieu within the nerve guides.					
32174148	6	92	theme	target	1290:1295	arg1	muscles					1297:1303	lower limb target muscles	1279:1303	lower limb target muscles	1279:1303	Upon explantation after 120 days, lower limb target muscles were harvested for calculation of muscle-weight ratios.					
32174148	10	93	theme	gene	1925:1928	arg1	expression					1930:1939	gene expression	1925:1939	gene expression of regeneration-supporting neurotrophic factors	1925:1987	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	12	94	theme	gene	2226:2229	arg1	profile					2242:2248	their gene expression profile	2220:2248	their gene expression profile	2220:2248	In addition, we demonstrate that our HAL, when used as a carrier system for co-transplanted SCs, changed their gene expression profile and deteriorated the pro-regenerative milieu within the nerve guides.					
32174148	12	95	theme	co-transplanted	2191:2205	arg1	SCs					2207:2209	co-transplanted SCs	2191:2209	co-transplanted SCs	2191:2209	In addition, we demonstrate that our HAL, when used as a carrier system for co-transplanted SCs, changed their gene expression profile and deteriorated the pro-regenerative milieu within the nerve guides.					
32174148	3	96	theme	cell-free	752:760	arg1	HA					762:763	cell-free HA	752:763	cell-free HA	752:763	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	6	97	theme	lower	1279:1283	arg1	muscles					1297:1303	lower limb target muscles	1279:1303	lower limb target muscles	1279:1303	Upon explantation after 120 days, lower limb target muscles were harvested for calculation of muscle-weight ratios.					
32174148	3	98	theme	growth	838:843	arg1	factor					845:850	fibroblast growth factor	827:850	fibroblast growth factor	827:850	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	1	99	theme	carrier	342:348	arg1	system					350:355	carrier system	342:355	carrier system	342:355	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	3	100	dep	cells	812:816	arg1	FGF2-SCs					884:891	FGF2-SCs	884:891	FGF2-SCs	884:891	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	3	100	dep	cells	812:816	arg1	cells					877:881	2-overexpressing Schwann cells	852:881	2-overexpressing Schwann cells (FGF2-SCs)	852:892	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	8	101	theme	ANG	1496:1498	arg1	treatment					1500:1508	only ANG treatment	1491:1508	only ANG treatment	1491:1508	After 120 days of recovery, only ANG treatment led to full motor recovery.					
32174148	5	102	theme	days	1219:1222	arg1	post-reconstruction					1224:1242	60, 90, and 120 days post-reconstruction	1203:1242	60, 90, and 120 days post-reconstruction	1203:1242	Motor recovery was surveyed by means of electrodiagnostic measurements at 60, 90, and 120 days post-reconstruction.					
32174148	0	103	theme	In	12:13	arg1	Evaluation					21:30	In Vivo and In Vitro Evaluation	0:30	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.	0:215	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	4	104	theme	15	955:956	arg1	mm					958:959	mm	958:959	mm	958:959	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	6	105	theme	ratios	1353:1358	arg1	calculation					1324:1334	calculation	1324:1334	calculation of muscle-weight ratios	1324:1358	Upon explantation after 120 days, lower limb target muscles were harvested for calculation of muscle-weight ratios.					
32174148	3	106	theme	2-overexpressing	852:867	arg1	FGF2-SCs					884:891	FGF2-SCs	884:891	FGF2-SCs	884:891	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	3	106	theme	2-overexpressing	852:867	arg1	cells					877:881	2-overexpressing Schwann cells	852:881	2-overexpressing Schwann cells (FGF2-SCs)	852:892	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	8	107	theme	full	1517:1520	arg1	recovery					1528:1535	full motor recovery	1517:1535	full motor recovery	1517:1535	After 120 days of recovery, only ANG treatment led to full motor recovery.					
32174148	10	108	theme	unexpected	1811:1820	arg1	result					1831:1836	this unexpected negative result	1806:1836	this unexpected negative result	1806:1836	Furthermore, complementary in vitro studies, conducted to elucidate the reason for this unexpected negative result, revealed that SCs and FGF2-SCs suspended within the hydrogel relatively downregulated gene expression of regeneration-supporting neurotrophic factors.					
32174148	1	109	theme	critical	444:451	arg1	model					466:470	a rat critical nerve defect model	438:470	a rat critical nerve defect model	438:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	110	theme	hyaluronic	281:290	arg1	HAL					315:317	HAL	315:317	HAL	315:317	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	110	theme	hyaluronic	281:290	arg1	hydrogel					305:312	a novel hyaluronic acid-laminin hydrogel	273:312	a novel hyaluronic acid-laminin hydrogel (HAL)	273:318	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	4	111	theme	Lewis	931:935	arg1	rats					937:940	female Lewis rats	924:940	female Lewis rats	924:940	We subjected female Lewis rats to immediate 15 mm sciatic nerve gap reconstruction and comprehensively compared axonal and functional regeneration parameters with the gold standard autologous nerve graft (ANG) repair.					
32174148	1	112	theme	defect	459:464	arg1	model					466:470	a rat critical nerve defect model	438:470	a rat critical nerve defect model	438:470	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	1	113	theme	hydrogel	305:312	arg1	suitability					258:268	the suitability	254:268	the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells	254:381	In the current study we investigated the suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within a composite chitosan-based nerve graft (CNG) in a rat critical nerve defect model.					
32174148	0	114	dep	In	12:13	arg1	Vitro					15:19	Vitro	15:19	Vitro	15:19	In Vivo and In Vitro Evaluation of a Novel Hyaluronic Acid-Laminin Hydrogel as Luminal Filler and Carrier System for Genetically Engineered Schwann Cells in Critical Gap Length Tubular Peripheral Nerve Graft in Rats.					
32174148	3	115	theme	two-chambered	648:660	arg1	guides					668:673	two-chambered nerve guides	648:673	two-chambered nerve guides	648:673	We filled hollow CNGs or two-chambered nerve guides with an inserted longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or additionally suspended either naïve Schwann cells (SCs) or fibroblast growth factor 2-overexpressing Schwann cells (FGF2-SCs) within the gels.					
32174148	5	116	theme	electrodiagnostic	1169:1185	arg1	measurements					1187:1198	electrodiagnostic measurements	1169:1198	electrodiagnostic measurements	1169:1198	Motor recovery was surveyed by means of electrodiagnostic measurements at 60, 90, and 120 days post-reconstruction.					
34688317	5	0	theme	tumor	734:738	arg1	diameter					740:747	tumor diameter	734:747	tumor diameter	734:747	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	6	1	theme	different	882:890	arg1	systems					904:910	different CP delivery systems	882:910	different CP delivery systems	882:910	We also studied the functional mechanism of different CP delivery systems.					
34688317	4	2	theme	immune	666:671	arg1	rejection					673:681	immune rejection	666:681	immune rejection	666:681	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	7	3	theme	PC-CP	966:970	arg1	higher					977:982	higher	977:982	higher	977:982	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	7	3	theme	PC-CP	966:970	arg1	efficiencies					950:961	The infiltration and transplantation efficiencies	913:961	The infiltration and transplantation efficiencies of PC-CP	913:970	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	1	4	theme	effective	137:145	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	7	5	theme	foreign	1010:1016	arg1	reaction					1023:1030	Less foreign body reaction	1005:1030	Less foreign body reaction	1005:1030	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	0	6	theme	Cancer	84:89	arg1	Therapy					91:97	Liver Cancer Therapy	78:97	Liver Cancer Therapy	78:97	A Poly-Chitosan and Cis-Platinum Conjugated Composite Nanoparticle System for Liver Cancer Therapy.					
34688317	7	7	theme	lower	1089:1093	arg1	reaction					1106:1113	lower macrophage reaction	1089:1113	lower macrophage reaction	1089:1113	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	7	8	theme	transplantation	934:948	arg1	higher					977:982	higher	977:982	higher	977:982	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	7	8	theme	transplantation	934:948	arg1	efficiencies					950:961	The infiltration and transplantation efficiencies	913:961	The infiltration and transplantation efficiencies of PC-CP	913:970	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	1	9	theme	nano-pole	147:155	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	3	10	theme	HepG2	315:319	arg1	cells					321:325	HepG2 cells	315:325	HepG2 cells	315:325	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	4	11	theme	Infiltration	466:477	arg1	efficiency					479:488	Infiltration efficiency	466:488	Infiltration efficiency	466:488	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	7	12	theme	fibroblast	1065:1074	arg1	reaction					1076:1083	less fibroblast reaction	1060:1083	less fibroblast reaction	1060:1083	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	8	13	theme	diameter	1179:1186	arg1	reduction					1188:1196	diameter reduction	1179:1196	diameter reduction	1179:1196	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	10	14	from	therapy	1424:1430	arg1	future					1439:1444	future	1439:1444	future	1439:1444	The PC system can better deliver and release drugs than PEI-CP, and may be a better choice for LC therapy in the future.					
34688317	8	15	theme	clinical	1120:1127	arg1	efficacy					1129:1136	The clinical efficacy	1116:1136	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction	1116:1196	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	8	15	theme	clinical	1120:1127	arg1	superior					1202:1209	superior	1202:1209	superior	1202:1209	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	6	16	theme	functional	858:867	arg1	mechanism					869:877	the functional mechanism	854:877	the functional mechanism of different CP delivery systems	854:910	We also studied the functional mechanism of different CP delivery systems.					
34688317	7	17	theme	infiltration	917:928	arg1	higher					977:982	higher	977:982	higher	977:982	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	7	17	theme	infiltration	917:928	arg1	efficiencies					950:961	The infiltration and transplantation efficiencies	913:961	The infiltration and transplantation efficiencies of PC-CP	913:970	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	2	18	theme	nano-pole	252:260	arg1	capsule					262:268	A novel nano-pole capsule	244:268	A novel nano-pole capsule	244:268	A novel nano-pole capsule was designed as a new vector for storing CP.					
34688317	2	18	theme	nano-pole	252:260	arg1	vector					292:297	a new vector	286:297	a new vector for storing CP	286:312	A novel nano-pole capsule was designed as a new vector for storing CP.					
34688317	9	19	contain	had	1257:1259	arg1	PC-CP					1251:1255	PC-CP	1251:1255	PC-CP	1251:1255	We demonstrated that PC-CP had a more significant alteration effect on mTOR, P-Ak, LC3 and P53.					
34688317	9	19	contain	had	1257:1259	arg2	effect					1291:1296	a more significant alteration effect	1261:1296	a more significant alteration effect	1261:1296	We demonstrated that PC-CP had a more significant alteration effect on mTOR, P-Ak, LC3 and P53.					
34688317	3	20	theme	poly-chitosan-cis-platinum	430:455	arg1	efficiency					372:381	the efficiency	368:381	the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP)	368:463	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	8	21	theme	PC-CP	1141:1145	arg1	efficacy					1129:1136	The clinical efficacy	1116:1136	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction	1116:1196	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	8	21	theme	PC-CP	1141:1145	arg1	superior					1202:1209	superior	1202:1209	superior	1202:1209	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	4	22	theme	macrophage	620:629	arg1	numbers					631:637	macrophage numbers	620:637	macrophage numbers	620:637	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	2	23	theme	novel	246:250	arg1	capsule					262:268	A novel nano-pole capsule	244:268	A novel nano-pole capsule	244:268	A novel nano-pole capsule was designed as a new vector for storing CP.					
34688317	2	23	theme	novel	246:250	arg1	vector					292:297	a new vector	286:297	a new vector for storing CP	286:312	A novel nano-pole capsule was designed as a new vector for storing CP.					
34688317	1	24	theme	capsule	157:163	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	5	25	theme	apoptosis	715:723	arg1	rate					725:728	apoptosis rate	715:728	apoptosis rate	715:728	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	9	26	theme	significant	1268:1278	arg1	effect					1291:1296	a more significant alteration effect	1261:1296	a more significant alteration effect	1261:1296	We demonstrated that PC-CP had a more significant alteration effect on mTOR, P-Ak, LC3 and P53.					
34688317	7	27	theme	less	1060:1063	arg1	reaction					1076:1083	less fibroblast reaction	1060:1083	less fibroblast reaction	1060:1083	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	4	28	theme	delivery	574:581	arg1	system					583:588	the delivery system	570:588	the delivery system	570:588	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	1	29	theme	loaded	165:170	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	3	30	theme	B6/J	333:336	arg1	model					344:348	a B6/J mouse model	331:348	a B6/J mouse model	331:348	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	9	31	theme	alteration	1280:1289	arg1	effect					1291:1296	a more significant alteration effect	1261:1296	a more significant alteration effect	1261:1296	We demonstrated that PC-CP had a more significant alteration effect on mTOR, P-Ak, LC3 and P53.					
34688317	1	32	theme	cis-platinum	172:183	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	3	33	theme	mouse	338:342	arg1	model					344:348	a B6/J mouse model	331:348	a B6/J mouse model	331:348	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	4	34	theme	foreign	687:693	arg1	reactions					700:708	foreign body reactions	687:708	foreign body reactions	687:708	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	8	35	theme	tumor	1159:1163	arg1	apoptosis					1165:1173	tumor apoptosis	1159:1173	tumor apoptosis	1159:1173	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	10	36	theme	PC	1330:1331	arg1	system					1333:1338	The PC system	1326:1338	The PC system	1326:1338	The PC system can better deliver and release drugs than PEI-CP, and may be a better choice for LC therapy in the future.					
34688317	1	37	theme	CP	186:187	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	7	38	theme	PC	1044:1045	arg1	system					1047:1052	PC system	1044:1052	PC system	1044:1052	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	7	39	theme	Less	1005:1008	arg1	reaction					1023:1030	Less foreign body reaction	1005:1030	Less foreign body reaction	1005:1030	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	4	40	theme	fibroblast	595:604	arg1	reactions					606:614	fibroblast reactions	595:614	fibroblast reactions	595:614	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	0	41	theme	Composite	44:52	arg1	System					67:72	Composite Nanoparticle System	44:72	Composite Nanoparticle System for Liver Cancer Therapy	44:97	A Poly-Chitosan and Cis-Platinum Conjugated Composite Nanoparticle System for Liver Cancer Therapy.					
34688317	5	42	dep	rate	725:728	arg1	The					711:713	The	711:713	The	711:713	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	3	43	theme	polyethyleneimine-cis-platinum	386:415	arg1	efficiency					372:381	the efficiency	368:381	the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP)	368:463	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	1	44	theme	transplantation	190:204	arg1	device					206:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device	134:211	an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy	134:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	5	45	theme	tumor	823:827	arg1	therapy					829:835	the tumor therapy	819:835	the tumor therapy	819:835	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	4	46	theme	transplantation	494:508	arg1	tests					521:525	transplantation efficiency tests	494:525	transplantation efficiency tests	494:525	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	5	47	theme	hepatocellular	752:765	arg1	cells					777:781	hepatocellular carcinoma cells	752:781	hepatocellular carcinoma cells	752:781	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	6	48	theme	delivery	895:902	arg1	systems					904:910	different CP delivery systems	882:910	different CP delivery systems	882:910	We also studied the functional mechanism of different CP delivery systems.					
34688317	5	49	theme	carcinoma	767:775	arg1	cells					777:781	hepatocellular carcinoma cells	752:781	hepatocellular carcinoma cells	752:781	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	5	50	theme	cells	777:781	arg1	rate					725:728	apoptosis rate	715:728	apoptosis rate	715:728	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	5	50	theme	cells	777:781	arg1	diameter					740:747	tumor diameter	734:747	tumor diameter	734:747	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	10	51	theme	LC	1421:1422	arg1	therapy					1424:1430	LC therapy	1421:1430	LC therapy in the future	1421:1444	The PC system can better deliver and release drugs than PEI-CP, and may be a better choice for LC therapy in the future.					
34688317	0	52	theme	Nanoparticle	54:65	arg1	System					67:72	Composite Nanoparticle System	44:72	Composite Nanoparticle System for Liver Cancer Therapy	44:97	A Poly-Chitosan and Cis-Platinum Conjugated Composite Nanoparticle System for Liver Cancer Therapy.					
34688317	6	53	theme	systems	904:910	arg1	mechanism					869:877	the functional mechanism	854:877	the functional mechanism of different CP delivery systems	854:910	We also studied the functional mechanism of different CP delivery systems.					
34688317	7	54	theme	body	1018:1021	arg1	reaction					1023:1030	Less foreign body reaction	1005:1030	Less foreign body reaction	1005:1030	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	8	55	theme	apoptosis	1165:1173	arg1	terms					1150:1154	terms	1150:1154	terms of tumor apoptosis and diameter reduction	1150:1196	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	1	56	theme	study	116:120	arg1	aim					104:106	The aim	100:106	The aim of this study	100:120	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	4	57	theme	efficiency	510:519	arg1	tests					521:525	transplantation efficiency tests	494:525	transplantation efficiency tests	494:525	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	5	58	used	used	788:791	arg2	diameter					740:747	tumor diameter	734:747	tumor diameter	734:747	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	5	58	used	used	788:791	arg2	rate					725:728	apoptosis rate	715:728	apoptosis rate	715:728	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	4	59	theme	body	695:698	arg1	reactions					700:708	foreign body reactions	687:708	foreign body reactions	687:708	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	10	60	theme	better	1403:1408	arg1	choice					1410:1415	a better choice	1401:1415	a better choice for LC therapy in the future	1401:1444	The PC system can better deliver and release drugs than PEI-CP, and may be a better choice for LC therapy in the future.					
34688317	4	61	theme	system	583:588	arg1	performance					555:565	the performance	551:565	the performance of the delivery system	551:588	Infiltration efficiency and transplantation efficiency tests were performed to study the performance of the delivery system, and fibroblast reactions and macrophage numbers were observed, to test for immune rejection and foreign body reactions.					
34688317	3	62	used	used	355:358	arg2	cells					321:325	HepG2 cells	315:325	HepG2 cells	315:325	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	3	62	used	used	355:358	arg2	model					344:348	a B6/J mouse model	331:348	a B6/J mouse model	331:348	HepG2 cells and a B6/J mouse model were used to test the efficiency of polyethyleneimine-cis-platinum (PEI-CP) and poly-chitosan-cis-platinum (PC-CP).					
34688317	8	63	from	efficacy	1129:1136	arg1	terms					1150:1154	terms	1150:1154	terms of tumor apoptosis and diameter reduction	1150:1196	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	2	64	theme	new	288:290	arg1	capsule					262:268	A novel nano-pole capsule	244:268	A novel nano-pole capsule	244:268	A novel nano-pole capsule was designed as a new vector for storing CP.					
34688317	2	64	theme	new	288:290	arg1	vector					292:297	a new vector	286:297	a new vector for storing CP	286:312	A novel nano-pole capsule was designed as a new vector for storing CP.					
34688317	8	65	theme	reduction	1188:1196	arg1	terms					1150:1154	terms	1150:1154	terms of tumor apoptosis and diameter reduction	1150:1196	The clinical efficacy of PC-CP in terms of tumor apoptosis and diameter reduction was superior to that of PEI-CP.					
34688317	1	66	theme	liver	217:221	arg1	cancer					223:228	liver cancer	217:228	liver cancer (LC) therapy	217:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	1	66	theme	liver	217:221	arg1	LC					231:232	LC	231:232	LC	231:232	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	0	67	theme	Liver	78:82	arg1	Therapy					91:97	Liver Cancer Therapy	78:97	Liver Cancer Therapy	78:97	A Poly-Chitosan and Cis-Platinum Conjugated Composite Nanoparticle System for Liver Cancer Therapy.					
34688317	5	68	theme	therapy	829:835	arg1	effect					809:814	the effect	805:814	the effect of the tumor therapy	805:835	The apoptosis rate and tumor diameter of hepatocellular carcinoma cells were used to evaluate the effect of the tumor therapy.					
34688317	1	69	theme	cancer	223:228	arg1	therapy					235:241	liver cancer (LC) therapy	217:241	liver cancer (LC) therapy	217:241	The aim of this study was to test an effective nano-pole capsule loaded cis-platinum (CP) transplantation device for liver cancer (LC) therapy.					
34688317	7	70	theme	macrophage	1095:1104	arg1	reaction					1106:1113	lower macrophage reaction	1089:1113	lower macrophage reaction	1089:1113	The infiltration and transplantation efficiencies of PC-CP were higher than that of PEI-CP; Less foreign body reaction appeared in PC system, with less fibroblast reaction and lower macrophage reaction.					
34688317	6	71	theme	CP	892:893	arg1	systems					904:910	different CP delivery systems	882:910	different CP delivery systems	882:910	We also studied the functional mechanism of different CP delivery systems.					
32135251	0	0	from	adsorption	15:24	arg1	bio-composite					57:69	magnetic alginate/rice husk bio-composite	29:69	magnetic alginate/rice husk bio-composite	29:69	Methylene blue adsorption on magnetic alginate/rice husk bio-composite.					
32135251	7	1	with	compatible	960:969	arg1	data					993:996	the experimental data	976:996	the experimental data	976:996	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	7	2	theme	isotherm	937:944	arg1	model					946:950	Freundlich isotherm model	926:950	Freundlich isotherm model	926:950	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	6	3	from	pH	704:705	arg1	efficiency					744:753	dye removal efficiency	732:753	dye removal efficiency of beads	732:762	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	10	4	theme	alginate/rice	1284:1296	arg1	bio-composite					1303:1315	the magnetic alginate/rice husk bio-composite	1271:1315	the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent	1271:1354	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	4	5	theme	beads	550:554	arg1	274.9 mg/g					574:583	274.9 mg/g	574:583	274.9 mg/g	574:583	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	4	5	theme	beads	550:554	arg1	capacity					520:527	Maximum experimental adsorption capacity	488:527	Maximum experimental adsorption capacity of the bio-composite beads	488:554	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	8	6	theme	methylene	1049:1057	arg1	adsorption					1035:1044	the adsorption	1031:1044	the adsorption of methylene blue	1031:1062	The kinetic studies showed that the adsorption of methylene blue can be well described by the fractal Brouers-Sotolongo kinetic model.					
32135251	10	7	theme	magnetic	1275:1282	arg1	bio-composite					1303:1315	the magnetic alginate/rice husk bio-composite	1271:1315	the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent	1271:1354	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	3	8	theme	alginate/rice	366:378	arg1	beads					395:399	magnetic alginate/rice husk composite beads	357:399	magnetic alginate/rice husk composite beads as an adsorbent	357:415	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	2	9	theme	composite	265:273	arg1	beads					275:279	magnetic alginate/rice husk composite beads	237:279	magnetic alginate/rice husk composite beads	237:279	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	1	10	used	used	176:179	arg2	beads					125:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	8	11	theme	kinetic	1003:1009	arg1	studies					1011:1017	The kinetic studies	999:1017	The kinetic studies	999:1017	The kinetic studies showed that the adsorption of methylene blue can be well described by the fractal Brouers-Sotolongo kinetic model.					
32135251	10	12	theme	removal	1397:1403	arg1	steps					1405:1409	cationic dye removal steps	1384:1409	cationic dye removal steps in the environmental engineering applications	1384:1455	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	3	13	theme	husk	380:383	arg1	beads					395:399	magnetic alginate/rice husk composite beads	357:399	magnetic alginate/rice husk composite beads as an adsorbent	357:415	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	4	14	theme	experimental	496:507	arg1	274.9 mg/g					574:583	274.9 mg/g	574:583	274.9 mg/g	574:583	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	4	14	theme	experimental	496:507	arg1	capacity					520:527	Maximum experimental adsorption capacity	488:527	Maximum experimental adsorption capacity of the bio-composite beads	488:554	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	1	15	theme	Magnetic	72:79	arg1	beads					125:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	10	16	theme	dye	1393:1395	arg1	steps					1405:1409	cationic dye removal steps	1384:1409	cationic dye removal steps in the environmental engineering applications	1384:1455	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	4	17	theme	Maximum	488:494	arg1	274.9 mg/g					574:583	274.9 mg/g	574:583	274.9 mg/g	574:583	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	4	17	theme	Maximum	488:494	arg1	capacity					520:527	Maximum experimental adsorption capacity	488:527	Maximum experimental adsorption capacity of the bio-composite beads	488:554	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	5	18	theme	MB	653:654	arg1	concentration					656:668	initial MB concentration	645:668	initial MB concentration	645:668	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	19	theme	initial	645:651	arg1	concentration					656:668	initial MB concentration	645:668	initial MB concentration	645:668	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	1	20	theme	alginate/rice	81:93	arg1	beads					125:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	6	21	theme	removal	736:742	arg1	efficiency					744:753	dye removal efficiency	732:753	dye removal efficiency of beads	732:762	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	3	22	theme	magnetic	357:364	arg1	beads					395:399	magnetic alginate/rice husk composite beads	357:399	magnetic alginate/rice husk composite beads as an adsorbent	357:415	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	6	23	theme	removal	822:828	arg1	efficiency					830:839	removal efficiency	822:839	removal efficiency	822:839	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	1	24	theme	husk	95:98	arg1	beads					125:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	6	25	theme	dye	732:734	arg1	efficiency					744:753	dye removal efficiency	732:753	dye removal efficiency of beads	732:762	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	1	26	theme	methylene	185:193	arg1	removal					205:211	methylene blue (MB) removal	185:211	methylene blue (MB) removal	185:211	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	0	27	theme	blue	10:13	arg1	Methylene					0:8	Methylene blue	0:13	Methylene blue	0:13	Methylene blue adsorption on magnetic alginate/rice husk bio-composite.					
32135251	4	28	theme	adsorption	509:518	arg1	274.9 mg/g					574:583	274.9 mg/g	574:583	274.9 mg/g	574:583	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	4	28	theme	adsorption	509:518	arg1	capacity					520:527	Maximum experimental adsorption capacity	488:527	Maximum experimental adsorption capacity of the bio-composite beads	488:554	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	8	29	theme	kinetic	1119:1125	arg1	model					1127:1131	the fractal Brouers-Sotolongo kinetic model	1089:1131	the fractal Brouers-Sotolongo kinetic model	1089:1131	The kinetic studies showed that the adsorption of methylene blue can be well described by the fractal Brouers-Sotolongo kinetic model.					
32135251	2	30	theme	TGA	319:321	arg1	techniques					323:332	FTIR, SEM-EDS and TGA techniques	301:332	FTIR, SEM-EDS and TGA techniques	301:332	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	9	31	theme	thermodynamic	1138:1150	arg1	calculations					1152:1163	The thermodynamic calculations	1134:1163	The thermodynamic calculations	1134:1163	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	1	32	theme	blue	195:198	arg1	methylene					185:193	methylene blue	185:198	methylene blue (MB) removal	185:211	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	1	32	theme	blue	195:198	arg1	MB					201:202	MB	201:202	MB	201:202	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	8	33	theme	Brouers-Sotolongo	1101:1117	arg1	model					1127:1131	the fractal Brouers-Sotolongo kinetic model	1089:1131	the fractal Brouers-Sotolongo kinetic model	1089:1131	The kinetic studies showed that the adsorption of methylene blue can be well described by the fractal Brouers-Sotolongo kinetic model.					
32135251	10	34	used	used	1375:1378	arg2	bio-composite					1303:1315	the magnetic alginate/rice husk bio-composite	1271:1315	the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent	1271:1354	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	1	35	theme	m-ALG/RH	101:108	arg1	beads					125:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	3	36	theme	blue	443:446	arg1	removal					425:431	the removal	421:431	the removal methylene blue	421:446	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	3	37	theme	composite	385:393	arg1	beads					395:399	magnetic alginate/rice husk composite beads	357:399	magnetic alginate/rice husk composite beads as an adsorbent	357:415	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	7	38	theme	various	846:852	arg1	models					863:868	The various isotherm models	842:868	The various isotherm models	842:868	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	8	39	theme	blue	1059:1062	arg1	methylene					1049:1057	methylene blue	1049:1062	methylene blue	1049:1062	The kinetic studies showed that the adsorption of methylene blue can be well described by the fractal Brouers-Sotolongo kinetic model.					
32135251	2	40	theme	SEM-EDS	307:313	arg1	techniques					323:332	FTIR, SEM-EDS and TGA techniques	301:332	FTIR, SEM-EDS and TGA techniques	301:332	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	6	41	theme	significant	710:720	arg1	effect					722:727	no significant effect	707:727	no significant effect	707:727	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	6	42	from	decrease	810:817	arg1	efficiency					830:839	removal efficiency	822:839	removal efficiency	822:839	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	3	43	theme	beads	395:399	arg1	accomplishment					339:352	The accomplishment	335:352	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue	335:446	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	6	44	theme	ionic	786:790	arg1	strength					792:799	ionic strength	786:799	ionic strength	786:799	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	1	45	theme	bio-composite	111:123	arg1	beads					125:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads	72:129	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	7	46	theme	isotherm	854:861	arg1	models					863:868	The various isotherm models	842:868	The various isotherm models	842:868	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	10	47	from	steps	1405:1409	arg1	applications					1444:1455	the environmental engineering applications	1414:1455	the environmental engineering applications	1414:1455	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	0	48	theme	alginate/rice	38:50	arg1	bio-composite					57:69	magnetic alginate/rice husk bio-composite	29:69	magnetic alginate/rice husk bio-composite	29:69	Methylene blue adsorption on magnetic alginate/rice husk bio-composite.					
32135251	7	49	theme	adsorption	901:910	arg1	mechanism					912:920	the adsorption mechanism	897:920	the adsorption mechanism	897:920	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	7	49	theme	adsorption	901:910	arg1	compatible					960:969	compatible	960:969	compatible	960:969	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	2	50	theme	magnetic	237:244	arg1	beads					275:279	magnetic alginate/rice husk composite beads	237:279	magnetic alginate/rice husk composite beads	237:279	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	10	51	theme	cationic	1384:1391	arg1	steps					1405:1409	cationic dye removal steps	1384:1409	cationic dye removal steps in the environmental engineering applications	1384:1455	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	0	52	theme	magnetic	29:36	arg1	bio-composite					57:69	magnetic alginate/rice husk bio-composite	29:69	magnetic alginate/rice husk bio-composite	29:69	Methylene blue adsorption on magnetic alginate/rice husk bio-composite.					
32135251	8	53	theme	fractal	1093:1099	arg1	model					1127:1131	the fractal Brouers-Sotolongo kinetic model	1089:1131	the fractal Brouers-Sotolongo kinetic model	1089:1131	The kinetic studies showed that the adsorption of methylene blue can be well described by the fractal Brouers-Sotolongo kinetic model.					
32135251	9	54	theme	blue	1190:1193	arg1	adsorption					1195:1204	methylene blue adsorption	1180:1204	methylene blue adsorption	1180:1204	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	9	54	theme	blue	1190:1193	arg1	nature					1239:1244	a spontaneous and exothermic nature	1210:1244	a spontaneous and exothermic nature	1210:1244	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	3	55	theme	aqueous	470:476	arg1	solution					478:485	aqueous solution	470:485	aqueous solution	470:485	The accomplishment of magnetic alginate/rice husk composite beads as an adsorbent for the removal methylene blue was investigated from aqueous solution.					
32135251	10	56	theme	environmental	1418:1430	arg1	applications					1444:1455	the environmental engineering applications	1414:1455	the environmental engineering applications	1414:1455	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	0	57	theme	husk	52:55	arg1	bio-composite					57:69	magnetic alginate/rice husk bio-composite	29:69	magnetic alginate/rice husk bio-composite	29:69	Methylene blue adsorption on magnetic alginate/rice husk bio-composite.					
32135251	2	58	theme	Structural	214:223	arg1	analysis					225:232	Structural analysis	214:232	Structural analysis of magnetic alginate/rice husk composite beads	214:279	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	5	59	theme	various	590:596	arg1	temperature					629:639	temperature	629:639	temperature	629:639	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	59	theme	various	590:596	arg1	parameters					606:615	The various process parameters	586:615	The various process parameters such as pH, temperature and initial MB concentration	586:668	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	59	theme	various	590:596	arg1	pH					625:626	pH	625:626	pH	625:626	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	59	theme	various	590:596	arg1	concentration					656:668	initial MB concentration	645:668	initial MB concentration	645:668	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	7	60	theme	experimental	980:991	arg1	data					993:996	the experimental data	976:996	the experimental data	976:996	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32135251	2	61	theme	beads	275:279	arg1	analysis					225:232	Structural analysis	214:232	Structural analysis of magnetic alginate/rice husk composite beads	214:279	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	5	62	theme	process	598:604	arg1	temperature					629:639	temperature	629:639	temperature	629:639	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	62	theme	process	598:604	arg1	parameters					606:615	The various process parameters	586:615	The various process parameters such as pH, temperature and initial MB concentration	586:668	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	62	theme	process	598:604	arg1	pH					625:626	pH	625:626	pH	625:626	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	5	62	theme	process	598:604	arg1	concentration					656:668	initial MB concentration	645:668	initial MB concentration	645:668	The various process parameters such as pH, temperature and initial MB concentration optimized.					
32135251	9	63	theme	exothermic	1228:1237	arg1	adsorption					1195:1204	methylene blue adsorption	1180:1204	methylene blue adsorption	1180:1204	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	9	63	theme	exothermic	1228:1237	arg1	nature					1239:1244	a spontaneous and exothermic nature	1210:1244	a spontaneous and exothermic nature	1210:1244	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	4	64	theme	bio-composite	536:548	arg1	beads					550:554	the bio-composite beads	532:554	the bio-composite beads	532:554	Maximum experimental adsorption capacity of the bio-composite beads was calculated as 274.9 mg/g.					
32135251	2	65	theme	FTIR	301:304	arg1	techniques					323:332	FTIR, SEM-EDS and TGA techniques	301:332	FTIR, SEM-EDS and TGA techniques	301:332	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	9	66	theme	spontaneous	1212:1222	arg1	adsorption					1195:1204	methylene blue adsorption	1180:1204	methylene blue adsorption	1180:1204	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	9	66	theme	spontaneous	1212:1222	arg1	nature					1239:1244	a spontaneous and exothermic nature	1210:1244	a spontaneous and exothermic nature	1210:1244	The thermodynamic calculations indicated that methylene blue adsorption was a spontaneous and exothermic nature.					
32135251	1	67	theme	ionotropic	145:154	arg1	method					165:170	ionotropic gelation method	145:170	ionotropic gelation method	145:170	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	10	68	theme	engineering	1432:1442	arg1	applications					1444:1455	the environmental engineering applications	1414:1455	the environmental engineering applications	1414:1455	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	2	69	theme	husk	260:263	arg1	beads					275:279	magnetic alginate/rice husk composite beads	237:279	magnetic alginate/rice husk composite beads	237:279	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	1	70	theme	gelation	156:163	arg1	method					165:170	ionotropic gelation method	145:170	ionotropic gelation method	145:170	Magnetic alginate/rice husk (m-ALG/RH) bio-composite beads prepared with ionotropic gelation method and used for methylene blue (MB) removal.					
32135251	10	71	theme	husk	1298:1301	arg1	bio-composite					1303:1315	the magnetic alginate/rice husk bio-composite	1271:1315	the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent	1271:1354	The results showed that the magnetic alginate/rice husk bio-composite as low-cost and eco-friendly adsorbent can be effectively used for cationic dye removal steps in the environmental engineering applications.					
32135251	2	72	theme	alginate/rice	246:258	arg1	beads					275:279	magnetic alginate/rice husk composite beads	237:279	magnetic alginate/rice husk composite beads	237:279	Structural analysis of magnetic alginate/rice husk composite beads was performed using FTIR, SEM-EDS and TGA techniques.					
32135251	6	73	theme	beads	758:762	arg1	efficiency					744:753	dye removal efficiency	732:753	dye removal efficiency of beads	732:762	It was determined that pH no significant effect on dye removal efficiency of beads while temperature and ionic strength caused a decrease on removal efficiency.					
32135251	7	74	theme	Freundlich	926:935	arg1	model					946:950	Freundlich isotherm model	926:950	Freundlich isotherm model	926:950	The various isotherm models were applied for determine the adsorption mechanism and Freundlich isotherm model is more compatible with the experimental data.					
32091513	2	0	theme	pH	439:440	arg1	function					418:425	a function	416:425	a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions	416:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	0	theme	pH	439:440	arg1	adsorption					365:374	The adsorption	361:374	The adsorption of U(vi) by the CS/ASca	361:398	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	3	1	theme	sorption	651:658	arg1	thermodynamics					660:673	the sorption thermodynamics	647:673	the sorption thermodynamics	647:673	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	4	2	theme	maximum	797:803	arg1	g-1					898:900	434.64 mg g-1	888:900	434.64 mg g-1	888:900	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	2	theme	maximum	797:803	arg1	capacity					816:823	the maximum adsorption capacity	793:823	the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model)	793:882	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	6	3	dep	little	1290:1295	arg1	effect					1303:1308	effect	1303:1308	effect	1303:1308	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	1	4	theme	composite	163:171	arg1	CS/ASca					182:188	CS/ASca	182:188	CS/ASca	182:188	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	1	4	theme	composite	163:171	arg1	aerogel					173:179	A new chitosan/aluminum sludge composite aerogel	132:179	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity	132:274	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	5	5	theme	X-ray	1006:1010	arg1	spectroscopy					1026:1037	X-ray photoelectron spectroscopy	1006:1037	X-ray photoelectron spectroscopy	1006:1037	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	1	6	theme	high	251:254	arg1	capacity					267:274	high adsorption capacity	251:274	high adsorption capacity	251:274	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	2	7	theme	solution	430:437	arg1	pH					439:440	solution pH	430:440	solution pH	430:440	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	5	8	theme	ion	1061:1063	arg1	adsorption					1065:1074	uranyl ion adsorption	1054:1074	uranyl ion adsorption	1054:1074	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	1	9	theme	adsorption	256:265	arg1	capacity					267:274	high adsorption capacity	251:274	high adsorption capacity	251:274	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	0	10	theme	chitosan/aluminum	88:104	arg1	aerogel					123:129	a new chitosan/aluminum sludge composite aerogel	82:129	a new chitosan/aluminum sludge composite aerogel	82:129	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	6	11	theme	K	1276:1276	arg1	ions					1281:1284	K(i) ions	1276:1284	K(i) ions	1276:1284	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	0	12	theme	new	84:86	arg1	aerogel					123:129	a new chitosan/aluminum sludge composite aerogel	82:129	a new chitosan/aluminum sludge composite aerogel	82:129	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	3	13	theme	spontaneous	684:694	arg1	processes					708:716	spontaneous endothermic processes	684:716	spontaneous endothermic processes	684:716	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	4	14	theme	adsorption	805:814	arg1	g-1					898:900	434.64 mg g-1	888:900	434.64 mg g-1	888:900	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	14	theme	adsorption	805:814	arg1	capacity					816:823	the maximum adsorption capacity	793:823	the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model)	793:882	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	15	theme	434.64	888:893	arg1	mg					895:896	mg	895:896	mg	895:896	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	0	16	theme	composite	113:121	arg1	aerogel					123:129	a new chitosan/aluminum sludge composite aerogel	82:129	a new chitosan/aluminum sludge composite aerogel	82:129	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	3	17	theme	endothermic	696:706	arg1	processes					708:716	spontaneous endothermic processes	684:716	spontaneous endothermic processes	684:716	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	4	18	theme	mg	785:786	arg1	L-1					788:790	10-700 mg L-1	778:790	10-700 mg L-1	778:790	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	19	from	pH	724:725	arg1	g-1					898:900	434.64 mg g-1	888:900	434.64 mg g-1	888:900	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	19	from	pH	724:725	arg1	capacity					816:823	the maximum adsorption capacity	793:823	the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model)	793:882	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	5	20	from	groups	1164:1169	arg1	surface					1178:1184	the surface	1174:1184	the surface of the CS/ASca	1174:1199	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	6	21	theme	U	1329:1329	arg1	sorption					1317:1324	the sorption	1313:1324	the sorption of U(vi)	1313:1333	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	2	22	theme	U	379:379	arg1	function					418:425	a function	416:425	a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions	416:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	22	theme	U	379:379	arg1	adsorption					365:374	The adsorption	361:374	The adsorption of U(vi) by the CS/ASca	361:398	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	0	23	theme	sludge	106:111	arg1	aerogel					123:129	a new chitosan/aluminum sludge composite aerogel	82:129	a new chitosan/aluminum sludge composite aerogel	82:129	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	4	24	theme	10-700	778:783	arg1	mg					785:786	mg	785:786	mg	785:786	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	5	25	from	spectroscopy	1026:1037	arg1	Data					903:906	Data	903:906	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy	903:1037	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	0	26	from	adsorption	28:37	arg1	water					73:77	water	73:77	water	73:77	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	5	27	from	spectrometry	960:971	arg1	Data					903:906	Data	903:906	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy	903:1037	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	28	theme	photoelectron	1012:1024	arg1	spectroscopy					1026:1037	X-ray photoelectron spectroscopy	1006:1037	X-ray photoelectron spectroscopy	1006:1037	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	4	29	theme	L-1	788:790	arg1	concentrations					760:773	initial uranium concentrations	744:773	initial uranium concentrations of 10-700 mg L-1	744:790	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	29	theme	L-1	788:790	arg1	pH					724:725	a pH	722:725	a pH of 4, 308 K	722:737	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	7	30	theme	new	1371:1373	arg1	clues					1375:1379	new clues	1371:1379	new clues	1371:1379	This study provides new clues for the treatment of radioactive wastewater.					
32091513	5	31	theme	microscopy/energy	931:947	arg1	spectrometry					960:971	scanning electron microscopy/energy dispersive spectrometry	913:971	scanning electron microscopy/energy dispersive spectrometry	913:971	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	31	theme	microscopy/energy	931:947	arg1	infrared					992:999	Fourier-transform infrared	974:999	Fourier-transform infrared	974:999	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	1	32	dep	waterworks	344:353	arg1	AS					356:357	AS	356:357	AS	356:357	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	2	33	theme	uranium	499:505	arg1	ions					523:526	coexisting ions	512:526	coexisting ions	512:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	33	theme	uranium	499:505	arg1	temperature					460:470	temperature	460:470	temperature	460:470	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	33	theme	uranium	499:505	arg1	pH					439:440	solution pH	430:440	solution pH	430:440	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	33	theme	uranium	499:505	arg1	time					454:457	adsorption time	443:457	adsorption time	443:457	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	33	theme	uranium	499:505	arg1	concentrations					481:494	initial concentrations	473:494	initial concentrations of uranium	473:505	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	5	34	with	complexation	1114:1125	arg1	groups					1164:1169	the amino and hydroxyl groups	1141:1169	groups	1164:1169	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	4	35	theme	uranium	752:758	arg1	concentrations					760:773	initial uranium concentrations	744:773	initial uranium concentrations of 10-700 mg L-1	744:790	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	1	36	theme	good	202:205	arg1	selectivity					207:217	good selectivity	202:217	good selectivity	202:217	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	5	37	theme	uranyl	1054:1059	arg1	adsorption					1065:1074	uranyl ion adsorption	1054:1074	uranyl ion adsorption	1054:1074	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	Na					1265:1266	Na	1265:1266	Na(i)	1265:1269	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg2	no					1300:1301	no	1300:1301	no	1300:1301	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	Pb					1257:1258	Pb	1257:1258	Pb(ii)	1257:1262	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg2	little					1290:1295	little	1290:1295	little	1290:1295	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	i					1268:1268	i	1268:1268	i	1268:1268	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	ii					1260:1261	ii	1260:1261	ii	1260:1261	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	Mg					1249:1250	the Mg	1245:1250	the Mg(ii)	1245:1254	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	ions					1281:1284	K(i) ions	1276:1284	K(i) ions	1276:1284	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	6	38	contain	had	1286:1288	arg1	ii					1252:1253	ii	1252:1253	ii	1252:1253	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	5	39	theme	Fourier-transform	974:990	arg1	spectrometry					960:971	scanning electron microscopy/energy dispersive spectrometry	913:971	scanning electron microscopy/energy dispersive spectrometry	913:971	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	39	theme	Fourier-transform	974:990	arg1	infrared					992:999	Fourier-transform infrared	974:999	Fourier-transform infrared	974:999	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	7	40	theme	wastewater	1414:1423	arg1	treatment					1389:1397	the treatment	1385:1397	the treatment of radioactive wastewater	1385:1423	This study provides new clues for the treatment of radioactive wastewater.					
32091513	4	41	dep	U	844:844	arg1	vi					846:847	vi	846:847	vi	846:847	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	41	dep	U	844:844	arg1	simulated					851:859	simulated	851:859	simulated	851:859	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	42	theme	mg	895:896	arg1	g-1					898:900	434.64 mg g-1	888:900	434.64 mg g-1	888:900	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	42	theme	mg	895:896	arg1	capacity					816:823	the maximum adsorption capacity	793:823	the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model)	793:882	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	5	43	theme	U	1130:1130	arg1	complexation					1114:1125	the complexation	1110:1125	the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca	1110:1199	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	2	44	theme	concentrations	481:494	arg1	function					418:425	a function	416:425	a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions	416:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	44	theme	concentrations	481:494	arg1	adsorption					365:374	The adsorption	361:374	The adsorption of U(vi) by the CS/ASca	361:398	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	3	45	theme	systematic	533:542	arg1	batch					544:548	The systematic batch	529:548	The systematic batch experiments	529:560	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	0	46	theme	adsorption	28:37	arg1	mechanism					15:23	mechanism	15:23	mechanism	15:23	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	0	46	theme	adsorption	28:37	arg1	Efficiency					0:9	Efficiency	0:9	Efficiency	0:9	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	2	47	theme	initial	473:479	arg1	concentrations					481:494	initial concentrations	473:494	initial concentrations of uranium	473:505	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	0	48	from	water	73:77	arg1	adsorption					28:37	adsorption	28:37	adsorption of low-concentration uranium from water	28:77	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	0	48	from	water	73:77	arg1	uranium					60:66	low-concentration uranium	42:66	low-concentration uranium from water	42:77	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	3	49	theme	batch	544:548	arg1	experiments					550:560	The systematic batch experiments	529:560	The systematic batch experiments	529:560	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	1	50	theme	easy	220:223	arg1	potential					236:244	easy separation potential	220:244	easy separation potential	220:244	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	0	51	theme	uranium	60:66	arg1	adsorption					28:37	adsorption	28:37	adsorption of low-concentration uranium from water	28:77	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	2	52	theme	ions	523:526	arg1	function					418:425	a function	416:425	a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions	416:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	52	theme	ions	523:526	arg1	adsorption					365:374	The adsorption	361:374	The adsorption of U(vi) by the CS/ASca	361:398	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	7	53	theme	radioactive	1402:1412	arg1	wastewater					1414:1423	radioactive wastewater	1402:1423	radioactive wastewater	1402:1423	This study provides new clues for the treatment of radioactive wastewater.					
32091513	5	54	theme	scanning	913:920	arg1	spectrometry					960:971	scanning electron microscopy/energy dispersive spectrometry	913:971	scanning electron microscopy/energy dispersive spectrometry	913:971	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	54	theme	scanning	913:920	arg1	infrared					992:999	Fourier-transform infrared	974:999	Fourier-transform infrared	974:999	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	4	55	from	concentrations	760:773	arg1	g-1					898:900	434.64 mg g-1	888:900	434.64 mg g-1	888:900	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	55	from	concentrations	760:773	arg1	capacity					816:823	the maximum adsorption capacity	793:823	the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model)	793:882	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	0	56	theme	low-concentration	42:58	arg1	uranium					60:66	low-concentration uranium	42:66	low-concentration uranium from water	42:77	Efficiency and mechanism of adsorption of low-concentration uranium from water by a new chitosan/aluminum sludge composite aerogel.					
32091513	2	57	theme	coexisting	512:521	arg1	ions					523:526	coexisting ions	512:526	coexisting ions	512:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	3	58	theme	pseudo-second-order	616:634	arg1	model					636:640	a pseudo-second-order model	614:640	a pseudo-second-order model	614:640	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	5	59	theme	electron	922:929	arg1	spectrometry					960:971	scanning electron microscopy/energy dispersive spectrometry	913:971	scanning electron microscopy/energy dispersive spectrometry	913:971	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	59	theme	electron	922:929	arg1	infrared					992:999	Fourier-transform infrared	974:999	Fourier-transform infrared	974:999	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	3	60	theme	adsorption	578:587	arg1	kinetics					589:596	the adsorption kinetics	574:596	the adsorption kinetics	574:596	The systematic batch experiments reveal that the adsorption kinetics is described by a pseudo-second-order model, and the sorption thermodynamics involves spontaneous endothermic processes.					
32091513	6	61	dep	U	1329:1329	arg1	vi					1331:1332	vi	1331:1332	vi	1331:1332	In addition, our results demonstrated that the Mg(ii), Pb(ii), Na(i), and K(i) ions had little or no effect on the sorption of U(vi) on the CS/ASca.					
32091513	5	62	theme	dispersive	949:958	arg1	spectrometry					960:971	scanning electron microscopy/energy dispersive spectrometry	913:971	scanning electron microscopy/energy dispersive spectrometry	913:971	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	62	theme	dispersive	949:958	arg1	infrared					992:999	Fourier-transform infrared	974:999	Fourier-transform infrared	974:999	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	4	63	theme	K	737:737	arg1	concentrations					760:773	initial uranium concentrations	744:773	initial uranium concentrations of 10-700 mg L-1	744:790	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	63	theme	K	737:737	arg1	pH					724:725	a pH	722:725	a pH of 4, 308 K	722:737	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	64	theme	initial	744:750	arg1	concentrations					760:773	initial uranium concentrations	744:773	initial uranium concentrations of 10-700 mg L-1	744:790	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	2	65	theme	time	454:457	arg1	function					418:425	a function	416:425	a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions	416:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	65	theme	time	454:457	arg1	adsorption					365:374	The adsorption	361:374	The adsorption of U(vi) by the CS/ASca	361:398	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	1	66	theme	separation	225:234	arg1	potential					236:244	easy separation potential	220:244	easy separation potential	220:244	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	4	67	theme	CS/ASca	832:838	arg1	g-1					898:900	434.64 mg g-1	888:900	434.64 mg g-1	888:900	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	4	67	theme	CS/ASca	832:838	arg1	capacity					816:823	the maximum adsorption capacity	793:823	the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model)	793:882	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	5	68	dep	U	1130:1130	arg1	vi					1132:1133	vi	1132:1133	vi	1132:1133	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	1	69	theme	new	134:136	arg1	CS/ASca					182:188	CS/ASca	182:188	CS/ASca	182:188	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	1	69	theme	new	134:136	arg1	aerogel					173:179	A new chitosan/aluminum sludge composite aerogel	132:179	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity	132:274	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	5	70	theme	CS/ASca	1193:1199	arg1	surface					1178:1184	the surface	1174:1184	the surface of the CS/ASca	1174:1199	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	5	71	theme	amino	1145:1149	arg1	groups					1164:1169	the amino and hydroxyl groups	1141:1169	groups	1164:1169	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	2	72	theme	adsorption	443:452	arg1	time					454:457	adsorption time	443:457	adsorption time	443:457	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	73	dep	U	379:379	arg1	vi					381:382	vi	381:382	vi	381:382	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	1	74	theme	chitosan/aluminum	138:154	arg1	CS/ASca					182:188	CS/ASca	182:188	CS/ASca	182:188	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	1	74	theme	chitosan/aluminum	138:154	arg1	aerogel					173:179	A new chitosan/aluminum sludge composite aerogel	132:179	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity	132:274	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	4	75	theme	Langmuir	868:875	arg1	model					877:881	the Langmuir model	864:881	the Langmuir model	864:881	At a pH of 4, 308 K, and initial uranium concentrations of 10-700 mg L-1, the maximum adsorption capacity of the CS/ASca for U(vi) (simulated by the Langmuir model) was 434.64 mg g-1.					
32091513	1	76	theme	aluminum	323:330	arg1	sludge					332:337	aluminum sludge	323:337	aluminum sludge	323:337	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	5	77	theme	hydroxyl	1155:1162	arg1	groups					1164:1169	the amino and hydroxyl groups	1141:1169	groups	1164:1169	Data from scanning electron microscopy/energy dispersive spectrometry, Fourier-transform infrared, and X-ray photoelectron spectroscopy indicated that uranyl ion adsorption was predominantly associated with the complexation of U(vi) with the amino and hydroxyl groups on the surface of the CS/ASca.					
32091513	1	78	theme	sludge	156:161	arg1	CS/ASca					182:188	CS/ASca	182:188	CS/ASca	182:188	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	1	78	theme	sludge	156:161	arg1	aerogel					173:179	A new chitosan/aluminum sludge composite aerogel	132:179	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity	132:274	A new chitosan/aluminum sludge composite aerogel (CS/ASca) exhibiting good selectivity, easy separation potential, and high adsorption capacity was synthesized by combining chitosan (CS) and aluminum sludge from waterworks (AS).					
32091513	2	79	theme	temperature	460:470	arg1	function					418:425	a function	416:425	a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions	416:526	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
32091513	2	79	theme	temperature	460:470	arg1	adsorption					365:374	The adsorption	361:374	The adsorption of U(vi) by the CS/ASca	361:398	The adsorption of U(vi) by the CS/ASca was assessed as a function of solution pH, adsorption time, temperature, initial concentrations of uranium, and coexisting ions.					
33605476	2	0	theme	protein	484:490	arg1	source					474:479	source	474:479	source of protein	474:490	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	1	dep	extract	465:471	arg1	source					474:479	source	474:479	source of protein	474:490	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	4	2	theme	glucosamine	712:722	arg1	content					724:730	the glucosamine content	708:730	the glucosamine content in biomass of T. reesei	708:754	A regression model was developed to estimate the glucosamine content in biomass of T. reesei from the compositions of the lignocellulosic substrate.					
33605476	1	3	theme	Trichoderma	255:265	arg1	reesei					267:272	Trichoderma reesei TISTR3080	255:282	Trichoderma reesei TISTR3080	255:282	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	0	4	theme	lignocellulosic	73:87	arg1	substrates					89:98	lignocellulosic substrates	73:98	lignocellulosic substrates	73:98	Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates.					
33605476	5	5	with	validation	894:903	arg1	results					927:933	the experimental results	910:933	the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	910:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	4	6	theme	substrate	801:809	arg1	compositions					765:776	the compositions	761:776	the compositions of the lignocellulosic substrate	761:809	A regression model was developed to estimate the glucosamine content in biomass of T. reesei from the compositions of the lignocellulosic substrate.					
33605476	5	7	from	biomass	961:967	arg1	results					927:933	the experimental results	910:933	the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	910:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	1	8	theme	reesei	267:272	arg1	biomass					244:250	biomass	244:250	biomass of Trichoderma reesei TISTR3080	244:282	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	0	9	from	Estimation	0:9	arg1	biomass					29:35	biomass	29:35	biomass of Trichoderma reesei	29:57	Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates.					
33605476	2	10	theme	synthetic	300:308	arg1	media					324:328	A synthetic solid surface media	298:328	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein)	298:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	11	theme	different	341:349	arg1	ratios					351:356	different ratios	341:356	different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin,	341:427	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	12	theme	xylan	361:365	arg1	concentrations					441:454	various concentrations	433:454	various concentrations of yeast extract (source of protein)	433:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	12	theme	xylan	361:365	arg1	ratios					351:356	different ratios	341:356	different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin,	341:427	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	4	13	theme	lignocellulosic	785:799	arg1	substrate					801:809	the lignocellulosic substrate	781:809	the lignocellulosic substrate	781:809	A regression model was developed to estimate the glucosamine content in biomass of T. reesei from the compositions of the lignocellulosic substrate.					
33605476	5	14	theme	acceptable	815:824	arg1	%					849:849	not more than 10%	833:849	not more than 10%	833:849	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	5	14	theme	acceptable	815:824	arg1	error					826:830	An acceptable error	812:830	An acceptable error (not more than 10%) of the regression model	812:874	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	5	15	theme	reesei	975:980	arg1	biomass					961:967	biomass	961:967	biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	961:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	4	16	theme	regression	665:674	arg1	model					676:680	A regression model	663:680	A regression model	663:680	A regression model was developed to estimate the glucosamine content in biomass of T. reesei from the compositions of the lignocellulosic substrate.					
33605476	5	17	from	results	927:933	arg1	biomass					961:967	biomass	961:967	biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	961:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	4	18	theme	reesei	749:754	arg1	content					724:730	the glucosamine content	708:730	the glucosamine content in biomass of T. reesei	708:754	A regression model was developed to estimate the glucosamine content in biomass of T. reesei from the compositions of the lignocellulosic substrate.					
33605476	5	19	theme	regression	859:868	arg1	model					870:874	the regression model	855:874	the regression model	855:874	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	5	20	theme	glucosamine	938:948	arg1	content					950:956	glucosamine content	938:956	glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	938:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	2	21	theme	carboxymethyl	384:396	arg1	cellulose					409:417	cellulose	409:417	cellulose	409:417	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	21	theme	carboxymethyl	384:396	arg1	xylan					361:365	xylan	361:365	xylan (hemicellulose)	361:381	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	21	theme	carboxymethyl	384:396	arg1	cellulose					398:406	carboxymethyl cellulose	384:406	carboxymethyl cellulose (cellulose)	384:418	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	22	theme	various	433:439	arg1	concentrations					441:454	various concentrations	433:454	various concentrations of yeast extract (source of protein)	433:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	5	23	theme	lignocellulosic	996:1010	arg1	media					1026:1030	lignocellulosic solid surface media	996:1030	lignocellulosic solid surface media made from copra waste and banana peel	996:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	2	24	theme	surface	316:322	arg1	media					324:328	A synthetic solid surface media	298:328	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein)	298:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	0	25	theme	glucosamine	14:24	arg1	Estimation					0:9	Estimation	0:9	Estimation of glucosamine in biomass of Trichoderma reesei	0:57	Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates.					
33605476	2	26	used	used	498:501	arg2	media					324:328	A synthetic solid surface media	298:328	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein)	298:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	27	theme	solid	310:314	arg1	media					324:328	A synthetic solid surface media	298:328	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein)	298:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	5	28	theme	surface	1018:1024	arg1	media					1026:1030	lignocellulosic solid surface media	996:1030	lignocellulosic solid surface media made from copra waste and banana peel	996:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	4	29	from	content	724:730	arg1	biomass					735:741	biomass	735:741	biomass	735:741	A regression model was developed to estimate the glucosamine content in biomass of T. reesei from the compositions of the lignocellulosic substrate.					
33605476	3	30	theme	T.	644:645	arg1	biomass					654:660	T. reesei biomass	644:660	T. reesei biomass	644:660	Regression analysis identified significant individual and interaction factors that affected glucosamine quantity in T. reesei biomass.					
33605476	3	31	theme	reesei	647:652	arg1	biomass					654:660	T. reesei biomass	644:660	T. reesei biomass	644:660	Regression analysis identified significant individual and interaction factors that affected glucosamine quantity in T. reesei biomass.					
33605476	1	32	theme	glucosamine	221:231	arg1	content					233:239	the glucosamine content	217:239	the glucosamine content in biomass of Trichoderma reesei TISTR3080	217:282	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	0	33	theme	reesei	52:57	arg1	biomass					29:35	biomass	29:35	biomass of Trichoderma reesei	29:57	Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates.					
33605476	3	34	theme	interaction	586:596	arg1	factors					598:604	significant individual and interaction factors	559:604	factors	598:604	Regression analysis identified significant individual and interaction factors that affected glucosamine quantity in T. reesei biomass.					
33605476	1	35	theme	compositions	116:127	arg1	Effects					101:107	Effects	101:107	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080	101:282	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	2	36	theme	yeast	459:463	arg1	extract					465:471	yeast extract	459:471	yeast extract (source of protein)	459:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	5	37	theme	copra	1042:1046	arg1	waste					1048:1052	copra waste	1042:1052	copra waste	1042:1052	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	5	38	from	content	950:956	arg1	biomass					961:967	biomass	961:967	biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	961:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	2	39	contain	containing	330:339	arg1	media					324:328	A synthetic solid surface media	298:328	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein)	298:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	39	contain	containing	330:339	arg2	ratios					351:356	different ratios	341:356	different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin,	341:427	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	39	contain	containing	330:339	arg2	concentrations					441:454	various concentrations	433:454	various concentrations of yeast extract (source of protein)	433:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	1	40	from	content	233:239	arg1	biomass					244:250	biomass	244:250	biomass of Trichoderma reesei TISTR3080	244:282	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	5	41	theme	model	870:874	arg1	%					849:849	not more than 10%	833:849	not more than 10%	833:849	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	5	41	theme	model	870:874	arg1	error					826:830	An acceptable error	812:830	An acceptable error (not more than 10%) of the regression model	812:874	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	1	42	theme	lignocellulosic	132:146	arg1	substrate					148:156	lignocellulosic substrate	132:156	lignocellulosic substrate	132:156	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	5	43	theme	content	950:956	arg1	results					927:933	the experimental results	910:933	the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	910:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	3	44	theme	Regression	528:537	arg1	analysis					539:546	Regression analysis	528:546	Regression analysis	528:546	Regression analysis identified significant individual and interaction factors that affected glucosamine quantity in T. reesei biomass.					
33605476	1	45	theme	substrate	148:156	arg1	hemicellulose					168:180	hemicellulose	168:180	hemicellulose	168:180	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	1	45	theme	substrate	148:156	arg1	compositions					116:127	the compositions	112:127	the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein	112:212	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	1	45	theme	substrate	148:156	arg1	lignin					194:199	lignin	194:199	lignin	194:199	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	1	45	theme	substrate	148:156	arg1	protein					206:212	protein	206:212	protein	206:212	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	1	45	theme	substrate	148:156	arg1	cellulose					183:191	cellulose	183:191	cellulose	183:191	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	5	46	theme	experimental	914:925	arg1	results					927:933	the experimental results	910:933	the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel	910:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	5	47	theme	banana	1058:1063	arg1	peel					1065:1068	banana peel	1058:1068	banana peel	1058:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
33605476	3	48	theme	glucosamine	620:630	arg1	quantity					632:639	glucosamine quantity	620:639	glucosamine quantity	620:639	Regression analysis identified significant individual and interaction factors that affected glucosamine quantity in T. reesei biomass.					
33605476	1	49	from	Effects	101:107	arg1	content					233:239	the glucosamine content	217:239	the glucosamine content in biomass of Trichoderma reesei TISTR3080	217:282	Effects of the compositions of lignocellulosic substrate including hemicellulose, cellulose, lignin, and protein on the glucosamine content in biomass of Trichoderma reesei TISTR3080 were studied.					
33605476	2	50	theme	extract	465:471	arg1	concentrations					441:454	various concentrations	433:454	various concentrations of yeast extract (source of protein)	433:491	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	2	50	theme	extract	465:471	arg1	ratios					351:356	different ratios	341:356	different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin,	341:427	A synthetic solid surface media containing different ratios of xylan (hemicellulose), carboxymethyl cellulose (cellulose), lignin, and various concentrations of yeast extract (source of protein) were used to cultivated T. reesei.					
33605476	5	51	theme	solid	1012:1016	arg1	media					1026:1030	lignocellulosic solid surface media	996:1030	lignocellulosic solid surface media made from copra waste and banana peel	996:1068	An acceptable error (not more than 10%) of the regression model was obtained from validation with the experimental results of glucosamine content in biomass of T. reesei cultivated on lignocellulosic solid surface media made from copra waste and banana peel.					
34973777	0	0	theme	facile	105:110	arg1	approach					126:133	a facile and effective approach	103:133	a facile and effective approach	103:133	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	6	1	theme	kit	888:890	arg1	rating					892:897	kit rating	888:897	kit rating: 12/12	888:904	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	1	2	theme	biodegradable	265:277	arg1	coatings					279:286	biodegradable coatings	265:286	biodegradable coatings	265:286	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	7	3	theme	mechanical	1050:1059	arg1	properties					1061:1070	mechanical properties	1050:1070	mechanical properties	1050:1070	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	0	4	theme	effective	116:124	arg1	approach					126:133	a facile and effective approach	103:133	a facile and effective approach	103:133	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	6	5	theme	water	844:848	arg1	resistance					850:859	water resistance	844:859	water resistance (31 g/m2)	844:869	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	6	5	theme	water	844:848	arg1	31 g/m2					862:868	31 g/m2	862:868	31 g/m2	862:868	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	7	6	theme	barrier	1030:1036	arg1	properties					1038:1047	excellent barrier properties	1020:1047	excellent barrier properties	1020:1047	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	7	theme	food	1141:1144	arg1	fields					1160:1165	the food and packaging fields	1137:1165	the food and packaging fields	1137:1165	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	8	theme	fluorine-free	1076:1088	arg1	properties					1090:1099	fluorine-free properties	1076:1099	fluorine-free properties	1076:1099	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	1	9	theme	high	293:296	arg1	resistance					323:332	water- and oil- resistance	307:332	water- and oil- resistance	307:332	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	9	theme	high	293:296	arg1	barrier					298:304	high barrier	293:304	high barrier	293:304	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	7	10	theme	many	1116:1119	arg1	applications					1121:1132	many applications	1116:1132	many applications	1116:1132	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	1	11	theme	Degradable	136:145	arg1	materials					157:165	Degradable bio-based materials	136:165	Degradable bio-based materials	136:165	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	11	theme	Degradable	136:145	arg1	coatings					209:216	functional coatings	198:216	functional coatings	198:216	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	12	theme	functional	198:207	arg1	materials					157:165	Degradable bio-based materials	136:165	Degradable bio-based materials	136:165	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	12	theme	functional	198:207	arg1	coatings					209:216	functional coatings	198:216	functional coatings	198:216	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	6	13	theme	good	911:914	arg1	properties					927:936	good mechanical properties	911:936	good mechanical properties	911:936	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	0	14	theme	collagen/sodium	11:25	arg1	alginate/polyvinyl					27:44	Degradable collagen/sodium alginate/polyvinyl	0:44	Degradable collagen/sodium alginate/polyvinyl	0:44	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	7	15	theme	paper	1003:1007	arg1	method					1009:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	3	16	theme	pretreated	508:517	arg1	paper					526:530	Ca2+ pretreated filter paper	503:530	Ca2+ pretreated filter paper	503:530	CF and SA were mixed evenly and coated on Ca2+ pretreated filter paper.					
34973777	2	17	theme	collagen	388:395	arg1	fibers					397:402	collagen fibers	388:402	collagen fibers (CF)	388:407	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	2	17	theme	collagen	388:395	arg1	CF					405:406	CF	405:406	CF	405:406	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	0	18	theme	Degradable	0:9	arg1	alginate/polyvinyl					27:44	Degradable collagen/sodium alginate/polyvinyl	0:44	Degradable collagen/sodium alginate/polyvinyl	0:44	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	7	19	contain	have	1111:1114	arg1	method					1009:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	19	contain	have	1111:1114	arg2	applications					1121:1132	many applications	1116:1132	many applications	1116:1132	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	3	20	theme	filter	519:524	arg1	paper					526:530	Ca2+ pretreated filter paper	503:530	Ca2+ pretreated filter paper	503:530	CF and SA were mixed evenly and coated on Ca2+ pretreated filter paper.					
34973777	4	21	theme	sodium	611:616	arg1	alginate					618:625	sodium alginate	611:625	sodium alginate	611:625	It was mainly due to the electrostatic adsorption between collagen fibers and sodium alginate, and the crosslinking between the adsorption products and Ca2+.					
34973777	7	22	theme	simple	986:991	arg1	paper					1003:1007	simple composite paper	986:1007	simple composite paper	986:1007	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	2	23	theme	sodium	410:415	arg1	SA					427:428	SA	427:428	SA	427:428	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	2	23	theme	sodium	410:415	arg1	alginate					417:424	sodium alginate	410:424	sodium alginate (SA)	410:429	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	1	24	theme	water-	307:312	arg1	resistance					323:332	water- and oil- resistance	307:332	water- and oil- resistance	307:332	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	24	theme	water-	307:312	arg1	barrier					298:304	high barrier	293:304	high barrier	293:304	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	7	25	theme	composite	993:1001	arg1	paper					1003:1007	simple composite paper	986:1007	simple composite paper	986:1007	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	3	26	theme	Ca2+	503:506	arg1	paper					526:530	Ca2+ pretreated filter paper	503:530	Ca2+ pretreated filter paper	503:530	CF and SA were mixed evenly and coated on Ca2+ pretreated filter paper.					
34973777	4	27	theme	electrostatic	558:570	arg1	adsorption					572:581	the electrostatic adsorption	554:581	the electrostatic adsorption between collagen fibers and sodium alginate, and the crosslinking between the adsorption products and Ca2+	554:688	It was mainly due to the electrostatic adsorption between collagen fibers and sodium alginate, and the crosslinking between the adsorption products and Ca2+.					
34973777	0	28	theme	barrier	59:65	arg1	coating					67:73	high barrier coating	54:73	high barrier coating	54:73	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	6	29	theme	vapor	811:815	arg1	48 g/m2·24 h					829:840	48 g/m2·24 h	829:840	48 g/m2·24 h	829:840	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	6	29	theme	vapor	811:815	arg1	resistance					817:826	excellent water vapor resistance	795:826	excellent water vapor resistance (48 g/m2·24 h)	795:841	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	4	30	theme	collagen	591:598	arg1	fibers					600:605	collagen fibers	591:605	collagen fibers	591:605	It was mainly due to the electrostatic adsorption between collagen fibers and sodium alginate, and the crosslinking between the adsorption products and Ca2+.					
34973777	2	31	theme	polyvinyl	436:444	arg1	PVB					455:457	PVB	455:457	PVB	455:457	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	2	31	theme	polyvinyl	436:444	arg1	butyral					446:452	polyvinyl butyral	436:452	polyvinyl butyral (PVB)	436:458	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	1	32	theme	oil-	318:321	arg1	resistance					323:332	water- and oil- resistance	307:332	water- and oil- resistance	307:332	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	32	theme	oil-	318:321	arg1	barrier					298:304	high barrier	293:304	high barrier	293:304	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	0	33	theme	high	54:57	arg1	coating					67:73	high barrier coating	54:73	high barrier coating	54:73	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	6	34	theme	water	805:809	arg1	vapor					811:815	excellent water vapor	795:815	excellent water vapor resistance (48 g/m2·24 h)	795:841	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	0	35	with	butyral	46:52	arg1	water/oil-resistant					80:98	water/oil-resistant	80:98	water/oil-resistant	80:98	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	6	36	theme	oil	872:874	arg1	resistance					876:885	oil resistance	872:885	oil resistance (kit rating: 12/12)	872:905	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	6	37	theme	excellent	795:803	arg1	vapor					811:815	excellent water vapor	795:815	excellent water vapor resistance (48 g/m2·24 h)	795:841	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	2	38	theme	such	349:352	arg1	coatings					354:361	such coatings	349:361	such coatings	349:361	In this work, such coatings were fabricated by using collagen fibers (CF), sodium alginate (SA), and polyvinyl butyral (PVB).					
34973777	7	39	theme	friendly	972:979	arg1	method					1009:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	40	theme	excellent	1020:1028	arg1	properties					1038:1047	excellent barrier properties	1020:1047	excellent barrier properties	1020:1047	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	1	41	theme	bio-based	147:155	arg1	materials					157:165	Degradable bio-based materials	136:165	Degradable bio-based materials	136:165	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	41	theme	bio-based	147:155	arg1	coatings					209:216	functional coatings	198:216	functional coatings	198:216	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	7	42	theme	degradable	944:953	arg1	friendly					972:979	friendly	972:979	friendly	972:979	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	0	43	from	approach	126:133	arg1	butyral					46:52	butyral	46:52	butyral	46:52	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	1	44	theme	great	236:240	arg1	it					228:229	it	228:229	it	228:229	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	1	44	theme	great	236:240	arg1	challenge					242:250	a great challenge	234:250	a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance	234:332	Degradable bio-based materials have been widely considered as functional coatings, however, it is a great challenge to fabricate biodegradable coatings with high barrier, water- and oil- resistance.					
34973777	4	45	theme	adsorption	661:670	arg1	products					672:679	the adsorption products	657:679	the adsorption products	657:679	It was mainly due to the electrostatic adsorption between collagen fibers and sodium alginate, and the crosslinking between the adsorption products and Ca2+.					
34973777	6	46	dep	resistance	876:885	arg1	rating					892:897	kit rating	888:897	kit rating: 12/12	888:904	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	7	47	theme	packaging	1150:1158	arg1	fields					1160:1165	the food and packaging fields	1137:1165	the food and packaging fields	1137:1165	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	6	48	dep	rating	892:897	arg1	12/12					900:904	12/12	900:904	12/12	900:904	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
34973777	7	49	contain	has	1016:1018	arg2	properties					1061:1070	mechanical properties	1050:1070	mechanical properties	1050:1070	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	49	contain	has	1016:1018	arg2	properties					1038:1047	excellent barrier properties	1020:1047	excellent barrier properties	1020:1047	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	49	contain	has	1016:1018	arg2	properties					1090:1099	fluorine-free properties	1076:1099	fluorine-free properties	1076:1099	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	7	49	contain	has	1016:1018	arg1	method					1009:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method	939:1014	This degradable, environmentally friendly, and simple composite paper method has excellent barrier properties, mechanical properties and fluorine-free properties, and will have many applications in the food and packaging fields.					
34973777	5	50	theme	PVB	702:704	arg1	solution					706:713	PVB solution	702:713	PVB solution	702:713	By coating PVB solution, the barrier performance was further improved.					
34973777	0	51	from	butyral	46:52	arg1	approach					126:133	a facile and effective approach	103:133	a facile and effective approach	103:133	Degradable collagen/sodium alginate/polyvinyl butyral high barrier coating with water/oil-resistant in a facile and effective approach.					
34973777	5	52	theme	barrier	720:726	arg1	performance					728:738	the barrier performance	716:738	the barrier performance	716:738	By coating PVB solution, the barrier performance was further improved.					
34973777	6	53	theme	mechanical	916:925	arg1	properties					927:936	good mechanical properties	911:936	good mechanical properties	911:936	Notably, the composite exhibited excellent water vapor resistance (48 g/m2·24 h), water resistance (31 g/m2), oil resistance (kit rating: 12/12) and good mechanical properties.					
33805875	7	0	theme	BC/Col	1209:1214	arg1	formation					1216:1224	The BC/Col formation	1205:1224	The BC/Col formation	1205:1224	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	4	1	from	formed-resulting	846:861	arg1	crystallinity					898:910	crystallinity	898:910	crystallinity of the material	898:926	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	4	1	from	formed-resulting	846:861	arg1	stability					884:892	increased thermal stability	866:892	increased thermal stability	866:892	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	2	2	theme	industry-and	388:399	arg1	oxBC					418:421	the fish industry-and pre-oxidated BC (oxBC)	379:422	the fish industry-and pre-oxidated BC (oxBC)	379:422	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	7	3	theme	water	1262:1266	arg1	permeability					1274:1285	water vapor permeability	1262:1285	water vapor permeability	1262:1285	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	2	4	theme	fish	383:386	arg1	oxBC					418:421	the fish industry-and pre-oxidated BC (oxBC)	379:422	the fish industry-and pre-oxidated BC (oxBC)	379:422	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	5	5	theme	H-bonded	1108:1115	arg1	OHs					1117:1119	H-bonded OHs	1108:1119	H-bonded OHs	1108:1119	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	7	6	theme	tensile	1241:1247	arg1	strength					1249:1256	tensile strength	1241:1256	tensile strength	1241:1256	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	3	7	dep	Fourier	602:608	arg1	transform					610:618	transform	610:618	transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests	610:711	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	3	8	theme	thermogravimetric	551:567	arg1	analysis					569:576	thermogravimetric analysis	551:576	thermogravimetric analysis	551:576	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	4	9	theme	cross-linked	824:835	arg1	ones					837:840	enzymatically cross-linked ones	810:840	enzymatically cross-linked ones	810:840	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	7	10	theme	enzymatic	1298:1306	arg1	cross-linking					1308:1320	enzymatic cross-linking	1298:1320	enzymatic cross-linking with TGGS	1298:1330	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	3	11	dep	transform	610:618	arg1	infrared					620:627	infrared	620:627	transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests	610:711	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	4	12	from	chains	728:733	arg1	BC/Col					753:758	uncross-linked BC/Col	738:758	uncross-linked BC/Col	738:758	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	6	13	theme	oxBC/Col	1161:1168	arg1	crystallinity					1144:1156	crystallinity	1144:1156	crystallinity	1144:1156	Thermal stability and crystallinity of oxBC/Col remain lower than those of BC/Col.					
33805875	6	13	theme	oxBC/Col	1161:1168	arg1	stability					1130:1138	Thermal stability	1122:1138	Thermal stability	1122:1138	Thermal stability and crystallinity of oxBC/Col remain lower than those of BC/Col.					
33805875	4	14	theme	new	788:790	arg1	bonds					801:805	new covalent bonds	788:805	new covalent bonds in enzymatically cross-linked ones	788:840	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	4	15	theme	material	919:926	arg1	crystallinity					898:910	crystallinity	898:910	crystallinity of the material	898:926	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	4	15	theme	material	919:926	arg1	stability					884:892	increased thermal stability	866:892	increased thermal stability	866:892	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	6	16	theme	Thermal	1122:1128	arg1	stability					1130:1138	Thermal stability	1122:1138	Thermal stability	1122:1138	Thermal stability and crystallinity of oxBC/Col remain lower than those of BC/Col.					
33805875	7	17	with	cross-linking	1308:1320	arg1	TGGS					1327:1330	TGGS	1327:1330	TGGS	1327:1330	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	2	18	theme	enzymatic	299:307	arg1	cross-linking-one					309:325	enzymatic cross-linking-one	299:325	enzymatic cross-linking-one recommended for the meat	299:350	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	2	19	used	used	290:293	arg2	transglutaminases					268:284	two transglutaminases	264:284	two transglutaminases	264:284	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	4	20	from	bonds	801:805	arg1	ones					837:840	enzymatically cross-linked ones	810:840	enzymatically cross-linked ones	810:840	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	4	21	theme	covalent	792:799	arg1	bonds					801:805	new covalent bonds	788:805	new covalent bonds in enzymatically cross-linked ones	788:840	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	1	22	theme	enzymatic	206:214	arg1	cross-linking					229:241	enzymatic and chemical cross-linking	206:241	enzymatic and chemical cross-linking	206:241	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	0	23	theme	Enzymatic	0:8	arg1	Cross-Linking					23:35	Enzymatic and Chemical Cross-Linking	0:35	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A	0:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	0	24	dep	Study	99:103	arg1	Cross-Linking					23:35	Enzymatic and Chemical Cross-Linking	0:35	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A	0:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	4	25	theme	polymer	720:726	arg1	chains					728:733	polymer chains	720:733	polymer chains in uncross-linked BC/Col	720:758	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	4	26	theme	thermal	876:882	arg1	stability					884:892	increased thermal stability	866:892	increased thermal stability	866:892	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	5	27	theme	carbonyl	1052:1059	arg1	groups					1061:1066	carbonyl groups	1052:1066	the carbonyl groups' formation	1048:1077	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	5	28	dep	bonds	939:943	arg1	cause					995:999	cause	995:999	cause	995:999	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	4	29	link	uncross-linked	738:751	arg1	BC/Col					753:758	uncross-linked BC/Col	738:758	uncross-linked BC/Col	738:758	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	1	30	theme	chemical	220:227	arg1	cross-linking					229:241	enzymatic and chemical cross-linking	206:241	enzymatic and chemical cross-linking	206:241	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	0	31	theme	Chemical	14:21	arg1	Cross-Linking					23:35	Enzymatic and Chemical Cross-Linking	0:35	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A	0:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	5	32	theme	secondary	1001:1009	arg1	groups					1019:1024	secondary alcohol groups	1001:1024	secondary alcohol groups to vanish	1001:1034	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	3	33	theme	barrier	699:705	arg1	tests					707:711	mechanical and water barrier tests	678:711	mechanical and water barrier tests	678:711	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	7	34	theme	BC	1290:1291	arg1	strength					1249:1256	tensile strength	1241:1256	tensile strength	1241:1256	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	7	34	theme	BC	1290:1291	arg1	permeability					1274:1285	water vapor permeability	1262:1285	water vapor permeability	1262:1285	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	2	35	theme	chemical	436:443	arg1	cross-linking					445:457	chemical cross-linking	436:457	chemical cross-linking	436:457	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	0	36	theme	Bacterial	40:48	arg1	Composites-A					74:85	Bacterial Cellulose/Fish Collagen Composites-A	40:85	Bacterial Cellulose/Fish Collagen Composites-A	40:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	5	37	theme	BC	981:982	arg1	oxidation					984:992	BC oxidation	981:992	BC oxidation	981:992	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	5	38	theme	OHs	1117:1119	arg1	number					1098:1103	the number	1094:1103	the number of H-bonded OHs	1094:1119	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	4	39	theme	uncross-linked	738:751	arg1	BC/Col					753:758	uncross-linked BC/Col	738:758	uncross-linked BC/Col	738:758	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	2	40	used	used	427:430	arg2	other					360:364	other	360:364	other	360:364	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	3	41	theme	obtained	481:488	arg1	composites					490:499	the obtained composites	477:499	the obtained composites	477:499	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	0	42	theme	Collagen	65:72	arg1	Composites-A					74:85	Bacterial Cellulose/Fish Collagen Composites-A	40:85	Bacterial Cellulose/Fish Collagen Composites-A	40:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	5	43	theme	D-glucose	957:965	arg1	units					967:971	D-glucose units	957:971	D-glucose units	957:971	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	3	44	theme	water	693:697	arg1	tests					707:711	mechanical and water barrier tests	678:711	mechanical and water barrier tests	678:711	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	4	45	from	crystallinity	898:910	arg1	formed-resulting					846:861	formed-resulting	846:861	formed-resulting	846:861	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	3	46	theme	scanning	521:528	arg1	microscopy					539:548	scanning electron microscopy	521:548	scanning electron microscopy	521:548	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	0	47	theme	Cellulose/Fish	50:63	arg1	Composites-A					74:85	Bacterial Cellulose/Fish Collagen Composites-A	40:85	Bacterial Cellulose/Fish Collagen Composites-A	40:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	7	48	theme	vapor	1268:1272	arg1	permeability					1274:1285	water vapor permeability	1262:1285	water vapor permeability	1262:1285	The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.					
33805875	1	49	theme	composites	180:189	arg1	properties					132:141	the properties	128:141	the properties of bacterial cellulose/fish collagen composites (BC/Col)	128:198	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	3	50	theme	electron	530:537	arg1	microscopy					539:548	scanning electron microscopy	521:548	scanning electron microscopy	521:548	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	1	51	theme	collagen	171:178	arg1	composites					180:189	bacterial cellulose/fish collagen composites	146:189	bacterial cellulose/fish collagen composites (BC/Col)	146:198	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	1	51	theme	collagen	171:178	arg1	BC/Col					192:197	BC/Col	192:197	BC/Col	192:197	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	4	52	from	stability	884:892	arg1	formed-resulting					846:861	formed-resulting	846:861	formed-resulting	846:861	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	1	53	theme	bacterial	146:154	arg1	composites					180:189	bacterial cellulose/fish collagen composites	146:189	bacterial cellulose/fish collagen composites (BC/Col)	146:198	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	1	53	theme	bacterial	146:154	arg1	BC/Col					192:197	BC/Col	192:197	BC/Col	192:197	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	5	54	theme	alcohol	1011:1017	arg1	groups					1019:1024	secondary alcohol groups	1001:1024	secondary alcohol groups to vanish	1001:1034	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	3	55	theme	X-ray	579:583	arg1	diffraction					585:595	X-ray diffraction	579:595	X-ray diffraction	579:595	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	3	56	theme	mechanical	678:687	arg1	tests					707:711	mechanical and water barrier tests	678:711	mechanical and water barrier tests	678:711	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	5	57	from	cleavage	945:952	arg1	units					967:971	D-glucose units	957:971	D-glucose units	957:971	The C2-C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups' formation, thus reducing the number of H-bonded OHs.					
33805875	1	58	theme	cellulose/fish	156:169	arg1	composites					180:189	bacterial cellulose/fish collagen composites	146:189	bacterial cellulose/fish collagen composites (BC/Col)	146:198	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	1	58	theme	cellulose/fish	156:169	arg1	BC/Col					192:197	BC/Col	192:197	BC/Col	192:197	This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking.					
33805875	4	59	link	cross-linked	824:835	arg1	ones					837:840	enzymatically cross-linked ones	810:840	enzymatically cross-linked ones	810:840	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	0	60	theme	Comparative	87:97	arg1	Study					99:103	Comparative Study	87:103	Comparative Study	87:103	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	3	61	theme	functional	653:662	arg1	properties					664:673	their functional properties	647:673	their functional properties	647:673	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	3	62	theme	composites	490:499	arg1	structure					464:472	The structure	460:472	The structure of the obtained composites	460:499	The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests.					
33805875	2	63	theme	BC	414:415	arg1	oxBC					418:421	the fish industry-and pre-oxidated BC (oxBC)	379:422	the fish industry-and pre-oxidated BC (oxBC)	379:422	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
33805875	0	64	theme	Composites-A	74:85	arg1	Cross-Linking					23:35	Enzymatic and Chemical Cross-Linking	0:35	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A	0:85	Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites-A Comparative Study.					
33805875	4	65	theme	increased	866:874	arg1	stability					884:892	increased thermal stability	866:892	increased thermal stability	866:892	While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed-resulting in increased thermal stability and crystallinity of the material.					
33805875	2	66	theme	pre-oxidated	401:412	arg1	oxBC					418:421	the fish industry-and pre-oxidated BC (oxBC)	379:422	the fish industry-and pre-oxidated BC (oxBC)	379:422	In our methodology, two transglutaminases are used for enzymatic cross-linking-one recommended for the meat and the other proposed for the fish industry-and pre-oxidated BC (oxBC) is used for chemical cross-linking.					
34893226	4	0	theme	bacteria	778:785	arg1	permeability					748:759	the cell wall permeability	734:759	the cell wall permeability of Gram-negative bacteria	734:785	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	6	1	theme	other	1065:1069	arg1	composites					1093:1102	other cationic photodynamic composites	1065:1102	other cationic photodynamic composites	1065:1102	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	5	2	theme	Gram-negative	1027:1039	arg1	bacteria					1041:1048	both Gram-positive and Gram-negative bacteria	1004:1048	both Gram-positive and Gram-negative bacteria	1004:1048	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	7	3	theme	broad-spectrum	1229:1242	arg1	M-CS-Ce6					1190:1197	M-CS-Ce6	1190:1197	M-CS-Ce6	1190:1197	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	7	3	theme	broad-spectrum	1229:1242	arg1	agent					1258:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	2	4	theme	great	398:402	arg1	necessity					404:412	great necessity	398:412	great necessity	398:412	Therefore, development of highly effective and broad-spectrum disinfecting alternatives to tackle this challenge is of great necessity.					
34893226	6	5	theme	photodynamic	1080:1091	arg1	composites					1093:1102	other cationic photodynamic composites	1065:1102	other cationic photodynamic composites	1065:1102	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	7	6	theme	promising	1210:1218	arg1	M-CS-Ce6					1190:1197	M-CS-Ce6	1190:1197	M-CS-Ce6	1190:1197	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	7	6	theme	promising	1210:1218	arg1	agent					1258:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	3	7	theme	different	430:438	arg1	structures					450:459	the different cell wall structures	426:459	the different cell wall structures of bacteria	426:471	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	2	8	theme	broad-spectrum	326:339	arg1	alternatives					354:365	highly effective and broad-spectrum disinfecting alternatives	305:365	highly effective and broad-spectrum disinfecting alternatives	305:365	Therefore, development of highly effective and broad-spectrum disinfecting alternatives to tackle this challenge is of great necessity.					
34893226	2	9	theme	disinfecting	341:352	arg1	alternatives					354:365	highly effective and broad-spectrum disinfecting alternatives	305:365	highly effective and broad-spectrum disinfecting alternatives	305:365	Therefore, development of highly effective and broad-spectrum disinfecting alternatives to tackle this challenge is of great necessity.					
34893226	6	10	theme	cationic	1071:1078	arg1	composites					1093:1102	other cationic photodynamic composites	1065:1102	other cationic photodynamic composites	1065:1102	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	4	11	theme	cell	738:741	arg1	permeability					748:759	the cell wall permeability	734:759	the cell wall permeability of Gram-negative bacteria	734:785	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	5	12	theme	enhanced	955:962	arg1	performance					984:994	significantly enhanced photo-antibacterial performance	941:994	significantly enhanced photo-antibacterial performance	941:994	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	5	13	dep	10 μg/mL	831:838	arg1	irradiated					852:861	irradiated	852:861	irradiated	852:861	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	2	14	theme	effective	312:320	arg1	alternatives					354:365	highly effective and broad-spectrum disinfecting alternatives	305:365	highly effective and broad-spectrum disinfecting alternatives	305:365	Therefore, development of highly effective and broad-spectrum disinfecting alternatives to tackle this challenge is of great necessity.					
34893226	4	15	theme	Gram-positive	693:705	arg1	bacteria					707:714	Gram-positive bacteria	693:714	Gram-positive bacteria	693:714	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	1	16	theme	antibacterial	201:213	arg1	agents					215:220	effective antibacterial agents	191:220	effective antibacterial agents	191:220	Bacterial infection accompanied by antibiotic resistance leads to the lack of effective antibacterial agents, which has become an imminent problem afflicting people.					
34893226	3	17	theme	wall	445:448	arg1	structures					450:459	the different cell wall structures	426:459	the different cell wall structures of bacteria	426:471	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	7	18	theme	antibacterial	1244:1256	arg1	M-CS-Ce6					1190:1197	M-CS-Ce6	1190:1197	M-CS-Ce6	1190:1197	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	7	18	theme	antibacterial	1244:1256	arg1	agent					1258:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	3	19	theme	molybdenum	577:586	arg1	M-CS-Ce6					619:626	M-CS-Ce6	619:626	M-CS-Ce6	619:626	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	19	theme	molybdenum	577:586	arg1	nanocomposites					603:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites	529:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	529:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	1	20	theme	agents	215:220	arg1	lack					183:186	the lack	179:186	the lack	179:186	Bacterial infection accompanied by antibiotic resistance leads to the lack of effective antibacterial agents, which has become an imminent problem afflicting people.					
34893226	5	21	theme	M-CS-Ce6	843:850	arg1	10 μg/mL					831:838	10 μg/mL	831:838	10 μg/mL	831:838	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	1	22	theme	Bacterial	113:121	arg1	infection					123:131	Bacterial infection	113:131	Bacterial infection accompanied by antibiotic resistance	113:168	Bacterial infection accompanied by antibiotic resistance leads to the lack of effective antibacterial agents, which has become an imminent problem afflicting people.					
34893226	3	23	theme	bacteria	464:471	arg1	structures					450:459	the different cell wall structures	426:459	the different cell wall structures of bacteria	426:471	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	0	24	theme	Photodynamic	0:11	arg1	chitosan					13:20	Photodynamic chitosan	0:20	Photodynamic chitosan	0:20	Photodynamic chitosan functionalized MoS2 nanocomposite with enhanced and broad-spectrum antibacterial activity.					
34893226	5	25	theme	Gram-positive	1009:1021	arg1	bacteria					1041:1048	both Gram-positive and Gram-negative bacteria	1004:1048	both Gram-positive and Gram-negative bacteria	1004:1048	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	6	26	contain	had	1114:1116	arg2	stronger					1118:1125	stronger	1118:1125	stronger	1118:1125	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	6	26	contain	had	1114:1116	arg1	M-CS-Ce6					1105:1112	M-CS-Ce6	1105:1112	M-CS-Ce6	1105:1112	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	6	26	contain	had	1114:1116	arg2	effect					1166:1171	broad-spectrum photo-antibacterial effect	1131:1171	broad-spectrum photo-antibacterial effect	1131:1171	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	5	27	theme	target	912:917	arg1	pathogens					919:927	the target pathogens	908:927	the target pathogens	908:927	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	0	28	theme	MoS2	37:40	arg1	nanocomposite					42:54	MoS2 nanocomposite	37:54	MoS2 nanocomposite	37:54	Photodynamic chitosan functionalized MoS2 nanocomposite with enhanced and broad-spectrum antibacterial activity.					
34893226	7	29	theme	safe	1224:1227	arg1	M-CS-Ce6					1190:1197	M-CS-Ce6	1190:1197	M-CS-Ce6	1190:1197	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	7	29	theme	safe	1224:1227	arg1	agent					1258:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	a promising and safe broad-spectrum antibacterial agent	1208:1262	Taken together, M-CS-Ce6 could be a promising and safe broad-spectrum antibacterial agent.					
34893226	3	30	theme	MoS2	597:600	arg1	M-CS-Ce6					619:626	M-CS-Ce6	619:626	M-CS-Ce6	619:626	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	30	theme	MoS2	597:600	arg1	nanocomposites					603:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites	529:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	529:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	5	31	theme	660 nm	866:871	arg1	laser					873:877	660 nm laser	866:877	660 nm laser for 5 min	866:887	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	3	32	theme	functionalized	562:575	arg1	M-CS-Ce6					619:626	M-CS-Ce6	619:626	M-CS-Ce6	619:626	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	32	theme	functionalized	562:575	arg1	nanocomposites					603:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites	529:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	529:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	1	33	theme	effective	191:199	arg1	agents					215:220	effective antibacterial agents	191:220	effective antibacterial agents	191:220	Bacterial infection accompanied by antibiotic resistance leads to the lack of effective antibacterial agents, which has become an imminent problem afflicting people.					
34893226	6	34	theme	photo-antibacterial	1146:1164	arg1	effect					1166:1171	broad-spectrum photo-antibacterial effect	1131:1171	broad-spectrum photo-antibacterial effect	1131:1171	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	3	35	theme	structures	450:459	arg1	view					418:421	view	418:421	view of the different cell wall structures of bacteria	418:471	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	6	36	theme	broad-spectrum	1131:1144	arg1	effect					1166:1171	broad-spectrum photo-antibacterial effect	1131:1171	broad-spectrum photo-antibacterial effect	1131:1171	Compared with other cationic photodynamic composites, M-CS-Ce6 had stronger and broad-spectrum photo-antibacterial effect.					
34893226	3	37	theme	photodynamic	486:497	arg1	system					513:518	photodynamic antibacterial system	486:518	photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	486:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	1	38	theme	imminent	243:250	arg1	problem					252:258	an imminent problem	240:258	an imminent problem afflicting people	240:276	Bacterial infection accompanied by antibiotic resistance leads to the lack of effective antibacterial agents, which has become an imminent problem afflicting people.					
34893226	3	39	theme	cell	440:443	arg1	structures					450:459	the different cell wall structures	426:459	the different cell wall structures of bacteria	426:471	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	1	40	theme	antibiotic	148:157	arg1	resistance					159:168	antibiotic resistance	148:168	antibiotic resistance	148:168	Bacterial infection accompanied by antibiotic resistance leads to the lack of effective antibacterial agents, which has become an imminent problem afflicting people.					
34893226	0	41	theme	enhanced	61:68	arg1	activity					103:110	enhanced and broad-spectrum antibacterial activity	61:110	enhanced and broad-spectrum antibacterial activity	61:110	Photodynamic chitosan functionalized MoS2 nanocomposite with enhanced and broad-spectrum antibacterial activity.					
34893226	5	42	theme	photo-antibacterial	964:982	arg1	performance					984:994	significantly enhanced photo-antibacterial performance	941:994	significantly enhanced photo-antibacterial performance	941:994	10 μg/mL of M-CS-Ce6 irradiated by 660 nm laser for 5 min, completely killed the target pathogens, exhibiting significantly enhanced photo-antibacterial performance against both Gram-positive and Gram-negative bacteria.					
34893226	4	43	theme	bacteria	707:714	arg1	cells					684:688	the cells	680:688	the cells of Gram-positive bacteria	680:714	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	4	44	theme	wall	743:746	arg1	permeability					748:759	the cell wall permeability	734:759	the cell wall permeability of Gram-negative bacteria	734:785	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	2	45	theme	alternatives	354:365	arg1	development					290:300	development	290:300	development of highly effective and broad-spectrum disinfecting alternatives to tackle this challenge	290:390	Therefore, development of highly effective and broad-spectrum disinfecting alternatives to tackle this challenge is of great necessity.					
34893226	4	46	theme	photo-antibacterial	803:821	arg1	effect					823:828	the photo-antibacterial effect	799:828	the photo-antibacterial effect	799:828	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	3	47	theme	sulfide	588:594	arg1	M-CS-Ce6					619:626	M-CS-Ce6	619:626	M-CS-Ce6	619:626	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	47	theme	sulfide	588:594	arg1	nanocomposites					603:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites	529:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	529:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	4	48	theme	Gram-negative	764:776	arg1	bacteria					778:785	Gram-negative bacteria	764:785	Gram-negative bacteria	764:785	The nanocomposite can not only allow Ce6 to enter the cells of Gram-positive bacteria, but also destroy the cell wall permeability of Gram-negative bacteria and enhance the photo-antibacterial effect.					
34893226	3	49	theme	chlorin	529:535	arg1	e6					537:538	chlorin e6	529:538	chlorin e6 (Ce6)	529:544	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	49	theme	chlorin	529:535	arg1	Ce6					541:543	Ce6	541:543	Ce6	541:543	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	0	50	theme	antibacterial	89:101	arg1	activity					103:110	enhanced and broad-spectrum antibacterial activity	61:110	enhanced and broad-spectrum antibacterial activity	61:110	Photodynamic chitosan functionalized MoS2 nanocomposite with enhanced and broad-spectrum antibacterial activity.					
34893226	3	51	theme	antibacterial	499:511	arg1	system					513:518	photodynamic antibacterial system	486:518	photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	486:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	52	theme	loaded	546:551	arg1	M-CS-Ce6					619:626	M-CS-Ce6	619:626	M-CS-Ce6	619:626	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	52	theme	loaded	546:551	arg1	nanocomposites					603:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites	529:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	529:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	0	53	theme	broad-spectrum	74:87	arg1	activity					103:110	enhanced and broad-spectrum antibacterial activity	61:110	enhanced and broad-spectrum antibacterial activity	61:110	Photodynamic chitosan functionalized MoS2 nanocomposite with enhanced and broad-spectrum antibacterial activity.					
34893226	3	54	dep	loaded	546:551	arg1	e6					537:538	chlorin e6	529:538	chlorin e6 (Ce6)	529:544	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	54	dep	loaded	546:551	arg1	Ce6					541:543	Ce6	541:543	Ce6	541:543	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	55	theme	chitosan	553:560	arg1	M-CS-Ce6					619:626	M-CS-Ce6	619:626	M-CS-Ce6	619:626	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
34893226	3	55	theme	chitosan	553:560	arg1	nanocomposites					603:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites	529:616	chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6)	529:627	In view of the different cell wall structures of bacteria, we designed photodynamic antibacterial system based on chlorin e6 (Ce6) loaded chitosan functionalized molybdenum sulfide (MoS2) nanocomposites (M-CS-Ce6).					
33787211	4	0	theme	myelin	987:992	arg1	MBP					1009:1011	MBP	1009:1011	MBP	1009:1011	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	4	0	theme	myelin	987:992	arg1	protein					1000:1006	myelin basic protein	987:1006	myelin basic protein (MBP) expression	987:1023	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	5	1	theme	sciatic	1159:1165	arg1	nerve					1167:1171	10 mm rat sciatic nerve	1149:1171	10 mm rat sciatic nerve	1149:1171	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	3	2	dep	improve	631:637	arg1	i					628:628	i	628:628	i	628:628	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	1	3	theme	clinical	145:152	arg1	conduit					116:122	The nerve guidance conduit	97:122	The nerve guidance conduit (NGC)	97:128	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	1	3	theme	clinical	145:152	arg1	strategy					154:161	a promising clinical strategy	133:161	a promising clinical strategy for regenerating the critical-sized peripheral nerve injury	133:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	5	4	theme	myelinated	1076:1085	arg1	axon					1087:1090	myelinated axon	1076:1090	myelinated axon in chitin/PEDOT groups	1076:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	5	5	from	Function	1026:1033	arg1	groups					1108:1113	chitin/PEDOT groups	1095:1113	chitin/PEDOT groups	1095:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	6	6	dep	attachment	1328:1337	arg1	the					1324:1326	the	1324:1326	the	1324:1326	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	4	7	theme	in	870:871	arg1	adhesion					891:898	the in vitro RSC-96 cell adhesion	866:898	the in vitro RSC-96 cell adhesion	866:898	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	5	8	from	autograft	1136:1144	arg1	nerve					1167:1171	10 mm rat sciatic nerve	1149:1171	10 mm rat sciatic nerve	1149:1171	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	4	9	theme	cell	942:945	arg1	S100					969:972	the Schwann cell activity-related gene S100	930:972	the Schwann cell activity-related gene S100	930:972	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	4	10	theme	cell	886:889	arg1	adhesion					891:898	the in vitro RSC-96 cell adhesion	866:898	the in vitro RSC-96 cell adhesion	866:898	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	3	11	theme	deacetylation	549:561	arg1	process					563:569	The partial deacetylation process	537:569	The partial deacetylation process of chitin for exposing the amino groups	537:609	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	3	12	theme	chitin	574:579	arg1	process					563:569	The partial deacetylation process	537:569	The partial deacetylation process of chitin for exposing the amino groups	537:609	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	7	13	theme	peripheral	1574:1583	arg1	restoration					1591:1601	the peripheral nerve restoration	1570:1601	the peripheral nerve restoration	1570:1601	This work provides an applicable chitin-based NGC material for accelerating the peripheral nerve restoration.					
33787211	4	14	theme	Schwann	934:940	arg1	S100					969:972	the Schwann cell activity-related gene S100	930:972	the Schwann cell activity-related gene S100	930:972	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	4	15	dep	in	870:871	arg1	vitro					873:877	vitro	873:877	vitro	873:877	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	4	16	theme	RSC-96	879:884	arg1	adhesion					891:898	the in vitro RSC-96 cell adhesion	866:898	the in vitro RSC-96 cell adhesion	866:898	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	6	17	theme	staining	1260:1267	arg1	analyses					1269:1276	toluidine blue staining analyses	1245:1276	toluidine blue staining analyses on the regenerated sciatic nerve	1245:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	5	18	from	nerve	1167:1171	arg1	autograft					1136:1144	autograft	1136:1144	autograft	1136:1144	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	7	19	theme	nerve	1585:1589	arg1	restoration					1591:1601	the peripheral nerve restoration	1570:1601	the peripheral nerve restoration	1570:1601	This work provides an applicable chitin-based NGC material for accelerating the peripheral nerve restoration.					
33787211	4	20	theme	gene	964:967	arg1	S100					969:972	the Schwann cell activity-related gene S100	930:972	the Schwann cell activity-related gene S100	930:972	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	3	21	theme	composite	735:743	arg1	strength					754:761	the composite hydrogel strength	731:761	the composite hydrogel strength	731:761	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	6	22	theme	Schwann	1372:1378	arg1	cells					1380:1384	Schwann cells	1372:1384	Schwann cells	1372:1384	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	23	theme	nerve	1474:1478	arg1	regeneration					1480:1491	the nerve regeneration	1470:1491	the nerve regeneration	1470:1491	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	4	24	theme	activity-related	947:962	arg1	S100					969:972	the Schwann cell activity-related gene S100	930:972	the Schwann cell activity-related gene S100	930:972	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	3	25	dep	offer	772:776	arg1	ii					768:769	ii	768:769	ii	768:769	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	2	26	theme	tetrapeptide	486:497	arg1	CRGD					516:519	CRGD	516:519	CRGD	516:519	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	26	theme	tetrapeptide	486:497	arg1	ChT-PEDOT-p					523:533	ChT-PEDOT-p	523:533	ChT-PEDOT-p	523:533	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	26	theme	tetrapeptide	486:497	arg1	Cys-Arg-Gly-Asp					499:513	cell adhesive tetrapeptide Cys-Arg-Gly-Asp	472:513	cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p)	472:534	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	3	27	theme	partial	541:547	arg1	process					563:569	The partial deacetylation process	537:569	The partial deacetylation process of chitin for exposing the amino groups	537:609	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	6	28	theme	vital	1411:1415	arg1	factors					1417:1423	the vital factors	1407:1423	the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration	1407:1491	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	28	theme	vital	1411:1415	arg1	angiogenesis					1390:1401	angiogenesis	1390:1401	angiogenesis	1390:1401	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	28	theme	vital	1411:1415	arg1	attachment					1328:1337	attachment	1328:1337	attachment	1328:1337	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	28	theme	vital	1411:1415	arg1	enhancement					1357:1367	proliferation enhancement	1343:1367	proliferation enhancement	1343:1367	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	0	29	theme	Chitin	14:19	arg1	Hydrogel					21:28	Biocompatible Chitin Hydrogel	0:28	Biocompatible Chitin Hydrogel	0:28	Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.					
33787211	5	30	theme	chitin/PEDOT	1095:1106	arg1	groups					1108:1113	chitin/PEDOT groups	1095:1113	chitin/PEDOT groups	1095:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	6	31	theme	regenerated	1285:1295	arg1	nerve					1305:1309	the regenerated sciatic nerve	1281:1309	the regenerated sciatic nerve	1281:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	5	32	theme	axon	1087:1090	arg1	thickness					1063:1071	thickness	1063:1071	thickness of myelinated axon in chitin/PEDOT groups	1063:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	5	32	theme	axon	1087:1090	arg1	Function					1026:1033	Function	1026:1033	Function of gastrocnemius muscle	1026:1057	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	2	33	theme	poly	394:397	arg1	nanoparticles					427:439	the conductive poly(3,4-ethylenedioxythiophene) nanoparticles	379:439	the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs)	379:451	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	33	theme	poly	394:397	arg1	NPs					448:450	PEDOT NPs	442:450	PEDOT NPs	442:450	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	0	34	theme	Biocompatible	0:12	arg1	Hydrogel					21:28	Biocompatible Chitin Hydrogel	0:28	Biocompatible Chitin Hydrogel	0:28	Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.					
33787211	5	35	theme	mm	1152:1153	arg1	nerve					1167:1171	10 mm rat sciatic nerve	1149:1171	10 mm rat sciatic nerve	1149:1171	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	7	36	theme	applicable	1516:1525	arg1	material					1544:1551	an applicable chitin-based NGC material	1513:1551	an applicable chitin-based NGC material	1513:1551	This work provides an applicable chitin-based NGC material for accelerating the peripheral nerve restoration.					
33787211	4	37	theme	protein	1000:1006	arg1	expression					1014:1023	myelin basic protein (MBP) expression	987:1023	myelin basic protein (MBP) expression	987:1023	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	1	38	theme	nerve	101:105	arg1	strategy					154:161	a promising clinical strategy	133:161	a promising clinical strategy for regenerating the critical-sized peripheral nerve injury	133:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	1	38	theme	nerve	101:105	arg1	NGC					125:127	NGC	125:127	NGC	125:127	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	1	38	theme	nerve	101:105	arg1	conduit					116:122	The nerve guidance conduit	97:122	The nerve guidance conduit (NGC)	97:128	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	2	39	theme	conductive	383:392	arg1	3,4-ethylenedioxythiophene					399:424	3,4-ethylenedioxythiophene	399:424	3,4-ethylenedioxythiophene	399:424	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	39	theme	conductive	383:392	arg1	poly					394:397	conductive poly	383:397	the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs)	379:451	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	6	40	theme	chitin/PEDOT	1433:1444	arg1	composite					1446:1454	the chitin/PEDOT composite	1429:1454	the chitin/PEDOT composite	1429:1454	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	5	41	theme	rat	1155:1157	arg1	nerve					1167:1171	10 mm rat sciatic nerve	1149:1171	10 mm rat sciatic nerve	1149:1171	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	1	42	theme	guidance	107:114	arg1	strategy					154:161	a promising clinical strategy	133:161	a promising clinical strategy for regenerating the critical-sized peripheral nerve injury	133:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	1	42	theme	guidance	107:114	arg1	NGC					125:127	NGC	125:127	NGC	125:127	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	1	42	theme	guidance	107:114	arg1	conduit					116:122	The nerve guidance conduit	97:122	The nerve guidance conduit (NGC)	97:128	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	2	43	theme	PEDOT	442:446	arg1	nanoparticles					427:439	the conductive poly(3,4-ethylenedioxythiophene) nanoparticles	379:439	the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs)	379:451	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	43	theme	PEDOT	442:446	arg1	NPs					448:450	PEDOT NPs	442:450	PEDOT NPs	442:450	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	5	44	from	axon	1087:1090	arg1	groups					1108:1113	chitin/PEDOT groups	1095:1113	chitin/PEDOT groups	1095:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	3	45	theme	hydrogel	745:752	arg1	strength					754:761	the composite hydrogel strength	731:761	the composite hydrogel strength	731:761	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	5	46	theme	muscle	1052:1057	arg1	thickness					1063:1071	thickness	1063:1071	thickness of myelinated axon in chitin/PEDOT groups	1063:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	5	46	theme	muscle	1052:1057	arg1	Function					1026:1033	Function	1026:1033	Function of gastrocnemius muscle	1026:1057	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	2	47	theme	hydrogel	290:297	arg1	film					299:302	the hydrogel film	286:302	the hydrogel film	286:302	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	1	48	theme	nerve	210:214	arg1	injury					216:221	the critical-sized peripheral nerve injury	180:221	the critical-sized peripheral nerve injury	180:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	0	49	theme	PEDOT	48:52	arg1	Nanoparticles					54:66	PEDOT Nanoparticles	48:66	PEDOT Nanoparticles for Peripheral Nerve Repair	48:94	Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.					
33787211	3	50	theme	electrostatic	643:655	arg1	interaction					657:667	the electrostatic interaction	639:667	the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength	639:761	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	1	51	theme	critical-sized	184:197	arg1	injury					216:221	the critical-sized peripheral nerve injury	180:221	the critical-sized peripheral nerve injury	180:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	2	52	theme	adhesive	477:484	arg1	CRGD					516:519	CRGD	516:519	CRGD	516:519	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	52	theme	adhesive	477:484	arg1	ChT-PEDOT-p					523:533	ChT-PEDOT-p	523:533	ChT-PEDOT-p	523:533	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	52	theme	adhesive	477:484	arg1	Cys-Arg-Gly-Asp					499:513	cell adhesive tetrapeptide Cys-Arg-Gly-Asp	472:513	cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p)	472:534	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	6	53	theme	western	1223:1229	arg1	blotting					1231:1238	western blotting	1223:1238	western blotting	1223:1238	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	54	theme	proliferation	1343:1355	arg1	factors					1417:1423	the vital factors	1407:1423	the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration	1407:1491	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	54	theme	proliferation	1343:1355	arg1	angiogenesis					1390:1401	angiogenesis	1390:1401	angiogenesis	1390:1401	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	54	theme	proliferation	1343:1355	arg1	attachment					1328:1337	attachment	1328:1337	attachment	1328:1337	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	54	theme	proliferation	1343:1355	arg1	enhancement					1357:1367	proliferation enhancement	1343:1367	proliferation enhancement	1343:1367	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	2	55	theme	cell	472:475	arg1	CRGD					516:519	CRGD	516:519	CRGD	516:519	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	55	theme	cell	472:475	arg1	ChT-PEDOT-p					523:533	ChT-PEDOT-p	523:533	ChT-PEDOT-p	523:533	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	2	55	theme	cell	472:475	arg1	Cys-Arg-Gly-Asp					499:513	cell adhesive tetrapeptide Cys-Arg-Gly-Asp	472:513	cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p)	472:534	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	5	56	theme	autograft	1136:1144	arg1	defect					1173:1178	the autograft in 10 mm rat sciatic nerve defect	1132:1178	the autograft in 10 mm rat sciatic nerve defect	1132:1178	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	5	57	from	thickness	1063:1071	arg1	groups					1108:1113	chitin/PEDOT groups	1095:1113	chitin/PEDOT groups	1095:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	4	58	theme	as-prepared	825:835	arg1	hydrogel					837:844	The as-prepared hydrogel	821:844	The as-prepared hydrogel	821:844	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	2	59	theme	nerve	338:342	arg1	regeneration					344:355	the impaired sciatic nerve regeneration	317:355	the impaired sciatic nerve regeneration	317:355	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	4	60	theme	basic	994:998	arg1	MBP					1009:1011	MBP	1009:1011	MBP	1009:1011	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	4	60	theme	basic	994:998	arg1	protein					1000:1006	myelin basic protein	987:1006	myelin basic protein (MBP) expression	987:1023	The as-prepared hydrogel remarkably promotes the in vitro RSC-96 cell adhesion and proliferation, as well as the Schwann cell activity-related gene S100, NF-200, and myelin basic protein (MBP) expression.					
33787211	5	61	from	groups	1108:1113	arg1	thickness					1063:1071	thickness	1063:1071	thickness of myelinated axon in chitin/PEDOT groups	1063:1113	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	5	61	from	groups	1108:1113	arg1	Function					1026:1033	Function	1026:1033	Function of gastrocnemius muscle	1026:1057	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	7	62	theme	chitin-based	1527:1538	arg1	material					1544:1551	an applicable chitin-based NGC material	1513:1551	an applicable chitin-based NGC material	1513:1551	This work provides an applicable chitin-based NGC material for accelerating the peripheral nerve restoration.					
33787211	3	63	theme	amino	598:602	arg1	groups					604:609	the amino groups	594:609	the amino groups	594:609	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	2	64	theme	sciatic	330:336	arg1	regeneration					344:355	the impaired sciatic nerve regeneration	317:355	the impaired sciatic nerve regeneration	317:355	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	5	65	theme	10	1149:1150	arg1	mm					1152:1153	mm	1152:1153	mm	1152:1153	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	6	66	theme	sciatic	1297:1303	arg1	nerve					1305:1309	the regenerated sciatic nerve	1281:1309	the regenerated sciatic nerve	1281:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	7	67	theme	NGC	1540:1542	arg1	material					1544:1551	an applicable chitin-based NGC material	1513:1551	an applicable chitin-based NGC material	1513:1551	This work provides an applicable chitin-based NGC material for accelerating the peripheral nerve restoration.					
33787211	2	68	theme	impaired	321:328	arg1	regeneration					344:355	the impaired sciatic nerve regeneration	317:355	the impaired sciatic nerve regeneration	317:355	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	6	69	from	immunohistochemistry	1201:1220	arg1	nerve					1305:1309	the regenerated sciatic nerve	1281:1309	the regenerated sciatic nerve	1281:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	70	from	analyses	1269:1276	arg1	nerve					1305:1309	the regenerated sciatic nerve	1281:1309	the regenerated sciatic nerve	1281:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	71	theme	cells	1380:1384	arg1	factors					1417:1423	the vital factors	1407:1423	the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration	1407:1491	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	71	theme	cells	1380:1384	arg1	angiogenesis					1390:1401	angiogenesis	1390:1401	angiogenesis	1390:1401	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	71	theme	cells	1380:1384	arg1	attachment					1328:1337	attachment	1328:1337	attachment	1328:1337	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	71	theme	cells	1380:1384	arg1	enhancement					1357:1367	proliferation enhancement	1343:1367	proliferation enhancement	1343:1367	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	72	theme	blue	1255:1258	arg1	analyses					1269:1276	toluidine blue staining analyses	1245:1276	toluidine blue staining analyses on the regenerated sciatic nerve	1245:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	73	from	Immunofluorescence	1181:1198	arg1	nerve					1305:1309	the regenerated sciatic nerve	1281:1309	the regenerated sciatic nerve	1281:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	6	74	from	blotting	1231:1238	arg1	nerve					1305:1309	the regenerated sciatic nerve	1281:1309	the regenerated sciatic nerve	1281:1309	Immunofluorescence, immunohistochemistry, western blotting, and toluidine blue staining analyses on the regenerated sciatic nerve explain that the attachment and proliferation enhancement of Schwann cells and angiogenesis are the vital factors for the chitin/PEDOT composite to facilitate the nerve regeneration.					
33787211	3	75	theme	peptide	799:805	arg1	modification					807:818	peptide modification	799:818	peptide modification	799:818	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	0	76	theme	Nerve	83:87	arg1	Repair					89:94	Peripheral Nerve Repair	72:94	Peripheral Nerve Repair	72:94	Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.					
33787211	5	77	theme	gastrocnemius	1038:1050	arg1	muscle					1052:1057	gastrocnemius muscle	1038:1057	gastrocnemius muscle	1038:1057	Function of gastrocnemius muscle and thickness of myelinated axon in chitin/PEDOT groups are analogous to the autograft in 10 mm rat sciatic nerve defect.					
33787211	2	78	theme	polysaccharide	243:256	arg1	chitin					258:263	the polysaccharide chitin	239:263	the polysaccharide chitin	239:263	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
33787211	1	79	theme	peripheral	199:208	arg1	injury					216:221	the critical-sized peripheral nerve injury	180:221	the critical-sized peripheral nerve injury	180:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	3	80	theme	active	782:787	arg1	sites					789:793	the active sites	778:793	the active sites for peptide modification	778:818	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	0	81	theme	Peripheral	72:81	arg1	Repair					89:94	Peripheral Nerve Repair	72:94	Peripheral Nerve Repair	72:94	Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.					
33787211	3	82	theme	charged	703:709	arg1	PEDOT					711:715	the negatively charged PEDOT	688:715	the negatively charged PEDOT for enhancing the composite hydrogel strength	688:761	The partial deacetylation process of chitin for exposing the amino groups is performed to (i) improve the electrostatic interaction between chitin and the negatively charged PEDOT for enhancing the composite hydrogel strength and (ii) offer the active sites for peptide modification.					
33787211	1	83	theme	promising	135:143	arg1	conduit					116:122	The nerve guidance conduit	97:122	The nerve guidance conduit (NGC)	97:128	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	1	83	theme	promising	135:143	arg1	strategy					154:161	a promising clinical strategy	133:161	a promising clinical strategy for regenerating the critical-sized peripheral nerve injury	133:221	The nerve guidance conduit (NGC) is a promising clinical strategy for regenerating the critical-sized peripheral nerve injury.					
33787211	2	84	used	used	268:271	arg2	chitin					258:263	the polysaccharide chitin	239:263	the polysaccharide chitin	239:263	In this study, the polysaccharide chitin is used to fabricate the hydrogel film for inducing the impaired sciatic nerve regeneration through incorporating the conductive poly(3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs) and modifying with cell adhesive tetrapeptide Cys-Arg-Gly-Asp (CRGD) (ChT-PEDOT-p).					
31962221	7	0	theme	Salmonella	1339:1348	arg1	CFU/ml					1323:1328	approximately 8 log10 CFU/ml	1301:1328	approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus	1301:1380	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	6	1	theme	%	1125:1125	arg1	LAE					1127:1129	0.5, 1 and 2.5% LAE	1111:1129	LAE	1127:1129	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	7	2	dep	L.	1351:1352	arg1	monocytogenes					1354:1366	L. monocytogenes	1351:1366	L. monocytogenes	1351:1366	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	3	theme	STEC	1333:1336	arg1	CFU/ml					1323:1328	approximately 8 log10 CFU/ml	1301:1328	approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus	1301:1380	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	4	contain	containing	1559:1568	arg1	CAFs					1554:1557	CAFs	1554:1557	CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C)	1554:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	4	contain	containing	1559:1568	arg2	LAE					1586:1588	0.5, 1, or 2.5% LAE	1570:1588	0.5, 1, or 2.5% LAE that were made into sachets/bags	1570:1621	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	4	contain	containing	1559:1568	arg2	vacuum					1624:1629	vacuum	1624:1629	vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C)	1624:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	5	dep	LAE	1586:1588	arg1	%					1584:1584	%	1584:1584	%	1584:1584	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	8	6	dep	containing	1758:1767	arg1	by					2057:2058	by	2057:2058	by	2057:2058	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	3	7	theme	coli	643:646	arg1	cocktails					605:613	cocktails	605:613	cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus)	605:751	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	9	8	theme	food	2241:2244	arg1	products					2246:2253	these food products	2235:2253	these food products	2235:2253	CAFs may be of interest to the meat and poultry industry to control foodborne pathogens associated with these food products.					
31962221	7	9	theme	preliminary	1256:1266	arg1	results					1268:1274	these preliminary results	1250:1274	these preliminary results	1250:1274	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	2	10	theme	pullulan	489:496	arg1	layer					498:502	the pullulan layer	485:502	the pullulan layer	485:502	Initially, CAFs were developed by incorporating thymol (T), nisin (N) and/or lauric arginate (LAE) into the pullulan layer and layering it on top of PE.					
31962221	1	11	theme	muscle	367:372	arg1	foods					374:378	muscle foods	367:378	muscle foods	367:378	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	1	12	theme	novel	195:199	arg1	CAF					231:233	CAF	231:233	CAF	231:233	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	1	12	theme	novel	195:199	arg1	film					225:228	A novel composite antimicrobial film	193:228	A novel composite antimicrobial film (CAF)	193:234	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	8	13	theme	raw	1933:1935	arg1	breast					1945:1950	raw chicken breast	1933:1950	raw chicken breast	1933:1950	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	6	14	theme	mechanical	1162:1171	arg1	characteristics					1173:1187	proper physical and mechanical characteristics	1142:1187	proper physical and mechanical characteristics	1142:1187	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	5	15	theme	physical	955:962	arg1	properties					979:988	desirable physical and mechanical properties	945:988	desirable physical and mechanical properties	945:988	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	1	16	theme	antimicrobial	211:223	arg1	CAF					231:233	CAF	231:233	CAF	231:233	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	1	16	theme	antimicrobial	211:223	arg1	film					225:228	A novel composite antimicrobial film	193:228	A novel composite antimicrobial film (CAF)	193:234	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	8	17	theme	RTE	2024:2026	arg1	breast					2035:2040	RTE turkey breast	2024:2040	RTE turkey breast	2024:2040	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	3	18	theme	Listeria	673:680	arg1	monocytogenes					682:694	Listeria monocytogenes	673:694	Listeria monocytogenes	673:694	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	3	18	theme	Listeria	673:680	arg1	coli					643:646	E. coli	640:646	E. coli (STEC)	640:653	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	6	19	theme	physical	1149:1156	arg1	characteristics					1173:1187	proper physical and mechanical characteristics	1142:1187	proper physical and mechanical characteristics	1142:1187	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	3	20	dep	aureus	734:739	arg1	aureus					745:750	S. aureus	742:750	S. aureus	742:750	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	3	21	theme	disk	756:759	arg1	DDAs					779:782	DDAs	779:782	DDAs	779:782	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	3	21	theme	disk	756:759	arg1	assays					771:776	disk diffusion assays	756:776	disk diffusion assays (DDAs)	756:783	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	8	22	dep	S.	2047:2048	arg1	aureus					2050:2055	S. aureus	2047:2055	S. aureus	2047:2055	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	7	23	dep	obtain	1487:1492	arg1	transferred					1539:1549	transferred	1539:1549	aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C)	1527:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	24	theme	raw	1428:1430	arg1	breast					1440:1445	raw chicken breast	1428:1445	raw chicken breast	1428:1445	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	25	dep	28 days	1702:1708	arg1	up					1696:1697	up	1696:1697	up	1696:1697	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	8	26	from	CFU/cm2	1908:1914	arg1	breast					1945:1950	raw chicken breast	1933:1950	raw chicken breast	1933:1950	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	3	27	theme	aureus	734:739	arg1	cocktails					605:613	cocktails	605:613	cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus)	605:751	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	6	28	theme	bacterial	1212:1220	arg1	pathogens					1222:1230	the four bacterial pathogens	1203:1230	the four bacterial pathogens in DDAs	1203:1238	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	5	29	theme	mechanical	968:977	arg1	properties					979:988	desirable physical and mechanical properties	945:988	desirable physical and mechanical properties	945:988	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	4	30	contain	containing	791:800	arg2	N					802:802	N	802:802	N	802:802	CAFs containing N were ineffective, while those containing T were effective for inhibiting the pathogens in DDAs.					
31962221	4	30	contain	containing	791:800	arg1	CAFs					786:789	CAFs	786:789	CAFs containing N	786:802	CAFs containing N were ineffective, while those containing T were effective for inhibiting the pathogens in DDAs.					
31962221	8	31	theme	turkey	2116:2121	arg1	breast					2123:2128	RTE turkey breast	2112:2128	RTE turkey breast	2112:2128	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	8	32	from	STEC	1799:1802	arg1	breast					2035:2040	RTE turkey breast	2024:2040	RTE turkey breast	2024:2040	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	6	33	from	pathogens	1222:1230	arg1	DDAs					1235:1238	DDAs	1235:1238	DDAs	1235:1238	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	7	34	theme	log10	1512:1516	arg1	CFU/cm2					1518:1524	approximately 6.6 log10 CFU/cm2	1494:1524	approximately 6.6 log10 CFU/cm2	1494:1524	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	1	35	theme	pullulan-based	249:262	arg1	biopolymer					264:273	a pullulan-based biopolymer	247:273	a pullulan-based biopolymer	247:273	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	7	36	theme	storage	1726:1732	arg1	28 days					1702:1708	28 days	1702:1708	28 days of refrigerated storage (4 °C)	1702:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	8	37	theme	%	1784:1784	arg1	LAE					1786:1788	0.5, 1, and 2.5% LAE	1769:1788	0.5, 1, and 2.5% LAE reduced	1769:1796	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	7	38	theme	aureus	1375:1380	arg1	CFU/ml					1323:1328	approximately 8 log10 CFU/ml	1301:1328	approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus	1301:1380	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	3	39	theme	CAFs	578:581	arg1	activity					552:559	The antimicrobial activity	534:559	The antimicrobial activity of the resulting CAFs	534:581	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	0	40	theme	pullulan-based	30:43	arg1	CAF					76:78	CAF	76:78	CAF	76:78	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	0	40	theme	pullulan-based	30:43	arg1	films					69:73	pullulan-based composite antimicrobial films	30:73	pullulan-based composite antimicrobial films (CAF)	30:79	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	8	41	from	CFU/cm2	2086:2092	arg1	breast					2123:2128	RTE turkey breast	2112:2128	RTE turkey breast	2112:2128	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	5	42	dep	solvents	996:1003	arg1	HCl					1006:1008	HCl	1006:1008	HCl	1006:1008	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	5	42	dep	solvents	996:1003	arg1	solvents					996:1003	solvents	996:1003	solvents (HCl and ethanol, respectively)	996:1035	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	5	42	dep	solvents	996:1003	arg1	ethanol					1014:1020	ethanol	1014:1020	ethanol	1014:1020	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	0	43	theme	antimicrobial	55:67	arg1	CAF					76:78	CAF	76:78	CAF	76:78	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	0	43	theme	antimicrobial	55:67	arg1	films					69:73	pullulan-based composite antimicrobial films	30:73	pullulan-based composite antimicrobial films (CAF)	30:79	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	9	44	theme	control	2191:2197	arg1	pathogens					2209:2217	control foodborne pathogens	2191:2217	control foodborne pathogens associated with these food products	2191:2253	CAFs may be of interest to the meat and poultry industry to control foodborne pathogens associated with these food products.					
31962221	8	45	theme	log10	1993:1997	arg1	CFU/cm2					1999:2005	1.12, 1.81 and 3.56 log10 CFU/cm2	1973:2005	1.12, 1.81 and 3.56 log10 CFU/cm2	1973:2005	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	8	46	dep	L.	1953:1954	arg1	monocytogenes					1956:1968	L. monocytogenes	1953:1968	L. monocytogenes	1953:1968	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	7	47	theme	microbial	1663:1671	arg1	populations					1673:1683	microbial populations	1663:1683	microbial populations determined up to 28 days of refrigerated storage (4 °C)	1663:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	0	48	theme	muscle	179:184	arg1	foods					186:190	muscle foods	179:190	muscle foods	179:190	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	5	49	theme	pullulan	1068:1075	arg1	binding					1057:1063	the binding	1053:1063	the binding of pullulan to PE	1053:1081	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	2	50	dep	layering	508:515	arg1	it					517:518	it	517:518	it	517:518	Initially, CAFs were developed by incorporating thymol (T), nisin (N) and/or lauric arginate (LAE) into the pullulan layer and layering it on top of PE.					
31962221	8	51	theme	turkey	2028:2033	arg1	breast					2035:2040	RTE turkey breast	2024:2040	RTE turkey breast	2024:2040	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	7	52	dep	%	1584:1584	arg1	1					1575:1575	1	1575:1575	1	1575:1575	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	52	dep	%	1584:1584	arg1	2.5					1581:1583	2.5	1581:1583	2.5	1581:1583	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	6	53	theme	2.5	1122:1124	arg1	LAE					1127:1129	0.5, 1 and 2.5% LAE	1111:1129	LAE	1127:1129	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	7	54	theme	log10	1317:1321	arg1	CFU/ml					1323:1328	approximately 8 log10 CFU/ml	1301:1328	approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus	1301:1380	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	6	55	theme	0.5	1111:1113	arg1	LAE					1127:1129	0.5, 1 and 2.5% LAE	1111:1129	LAE	1127:1129	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	7	56	theme	turkey	1470:1475	arg1	breast					1477:1482	turkey breast	1470:1482	turkey breast	1470:1482	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	8	57	theme	log10	1902:1906	arg1	CFU/cm2					1908:1914	2.03, 2.12 and 3.01 log10 CFU/cm2	1882:1914	2.03, 2.12 and 3.01 log10 CFU/cm2	1882:1914	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	0	58	theme	lauric	117:122	arg1	arginate					124:131	lauric arginate	117:131	lauric arginate	117:131	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	7	59	dep	vacuum	1624:1629	arg1	sealed					1641:1646	sealed	1641:1646	sealed	1641:1646	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	59	dep	vacuum	1624:1629	arg1	remaining					1653:1661	remaining	1653:1661	remaining microbial populations determined up to 28 days of refrigerated storage (4 °C)	1653:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	59	dep	vacuum	1624:1629	arg1	packaged					1631:1638	packaged	1631:1638	packaged	1631:1638	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	5	60	theme	desirable	945:953	arg1	properties					979:988	desirable physical and mechanical properties	945:988	desirable physical and mechanical properties	945:988	However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE.					
31962221	1	61	theme	composite	201:209	arg1	CAF					231:233	CAF	231:233	CAF	231:233	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	1	61	theme	composite	201:209	arg1	film					225:228	A novel composite antimicrobial film	193:228	A novel composite antimicrobial film (CAF)	193:234	A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods.					
31962221	0	62	theme	foodborne	143:151	arg1	pathogens					153:161	foodborne pathogens	143:161	foodborne pathogens associated with muscle foods	143:190	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	8	63	theme	log10	2080:2084	arg1	CFU/cm2					2086:2092	0.68, 2.02 and 3.43 log10 CFU/cm2	2060:2092	0.68, 2.02 and 3.43 log10 CFU/cm2	2060:2092	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	8	64	theme	chicken	1937:1943	arg1	breast					1945:1950	raw chicken breast	1933:1950	raw chicken breast	1933:1950	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	6	65	theme	proper	1142:1147	arg1	characteristics					1173:1187	proper physical and mechanical characteristics	1142:1187	proper physical and mechanical characteristics	1142:1187	Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs.					
31962221	3	66	theme	antimicrobial	538:550	arg1	activity					552:559	The antimicrobial activity	534:559	The antimicrobial activity of the resulting CAFs	534:581	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	3	67	theme	diffusion	761:769	arg1	DDAs					779:782	DDAs	779:782	DDAs	779:782	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	3	67	theme	diffusion	761:769	arg1	assays					771:776	disk diffusion assays	756:776	disk diffusion assays (DDAs)	756:783	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	7	68	theme	chicken	1432:1438	arg1	breast					1440:1445	raw chicken breast	1428:1445	raw chicken breast	1428:1445	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	69	dep	beef	1422:1425	arg1	breast					1477:1482	turkey breast	1470:1482	turkey breast	1470:1482	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	8	70	from	LAE	1786:1788	arg1	breast					2035:2040	RTE turkey breast	2024:2040	RTE turkey breast	2024:2040	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	8	71	theme	RTE	2112:2114	arg1	breast					2123:2128	RTE turkey breast	2112:2128	RTE turkey breast	2112:2128	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	2	72	theme	lauric	458:463	arg1	arginate					465:472	lauric arginate	458:472	lauric arginate (LAE)	458:478	Initially, CAFs were developed by incorporating thymol (T), nisin (N) and/or lauric arginate (LAE) into the pullulan layer and layering it on top of PE.					
31962221	2	72	theme	lauric	458:463	arg1	LAE					475:477	LAE	475:477	LAE	475:477	Initially, CAFs were developed by incorporating thymol (T), nisin (N) and/or lauric arginate (LAE) into the pullulan layer and layering it on top of PE.					
31962221	3	73	dep	L.	697:698	arg1	monocytogenes					700:712	L. monocytogenes	697:712	L. monocytogenes	697:712	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	7	74	theme	refrigerated	1713:1724	arg1	4 °C					1735:1738	4 °C	1735:1738	4 °C	1735:1738	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	7	74	theme	refrigerated	1713:1724	arg1	storage					1726:1732	refrigerated storage	1713:1732	refrigerated storage (4 °C)	1713:1739	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	9	75	theme	meat	2162:2165	arg1	industry					2179:2186	the meat and poultry industry	2158:2186	the meat and poultry industry to control foodborne pathogens associated with these food products	2158:2253	CAFs may be of interest to the meat and poultry industry to control foodborne pathogens associated with these food products.					
31962221	8	76	theme	log10	1827:1831	arg1	CFU/cm2					1833:1839	1.13, 1.33 and 2.88 log10 CFU/cm2	1807:1839	1.13, 1.33 and 2.88 log10 CFU/cm2	1807:1839	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	3	77	theme	resulting	568:576	arg1	CAFs					578:581	the resulting CAFs	564:581	the resulting CAFs	564:581	The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs).					
31962221	0	78	theme	composite	45:53	arg1	CAF					76:78	CAF	76:78	CAF	76:78	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	0	78	theme	composite	45:53	arg1	films					69:73	pullulan-based composite antimicrobial films	30:73	pullulan-based composite antimicrobial films (CAF)	30:79	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	9	79	theme	poultry	2171:2177	arg1	industry					2179:2186	the meat and poultry industry	2158:2186	the meat and poultry industry to control foodborne pathogens associated with these food products	2158:2253	CAFs may be of interest to the meat and poultry industry to control foodborne pathogens associated with these food products.					
31962221	0	80	theme	films	69:73	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	0	80	theme	films	69:73	arg1	Development					0:10	Development	0:10	Development	0:10	Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods.					
31962221	4	81	from	pathogens	881:889	arg1	DDAs					894:897	DDAs	894:897	DDAs	894:897	CAFs containing N were ineffective, while those containing T were effective for inhibiting the pathogens in DDAs.					
31962221	9	82	theme	foodborne	2199:2207	arg1	pathogens					2209:2217	control foodborne pathogens	2191:2217	control foodborne pathogens associated with these food products	2191:2253	CAFs may be of interest to the meat and poultry industry to control foodborne pathogens associated with these food products.					
31962221	4	83	contain	containing	834:843	arg1	those					828:832	those	828:832	those	828:832	CAFs containing N were ineffective, while those containing T were effective for inhibiting the pathogens in DDAs.					
31962221	4	83	contain	containing	834:843	arg2	T					845:845	T	845:845	T	845:845	CAFs containing N were ineffective, while those containing T were effective for inhibiting the pathogens in DDAs.					
31962221	8	84	theme	beef	1862:1865	arg1	raw					1858:1860	raw beef	1858:1865	raw beef	1858:1865	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
31962221	7	85	theme	L.	1351:1352	arg1	CFU/ml					1323:1328	approximately 8 log10 CFU/ml	1301:1328	approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus	1301:1380	Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C).					
31962221	8	86	from	CFU/cm2	1833:1839	arg1	raw					1858:1860	raw beef	1858:1865	raw beef	1858:1865	By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast.					
33109345	6	0	theme	median	912:917	arg1	increase					943:950	a median two-fold (IQR: 1.7-2.4) increase	910:950	a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia	910:972	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	3	1	theme	double	503:508	arg1	network					510:516	a double network	501:516	a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive	501:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	8	2	from	model	1356:1360	arg1	areas					1305:1309	specific areas	1296:1309	specific areas of the spina bifida defect in a fetal rabbit model	1296:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	1	3	theme	liquid	258:263	arg1	environments					265:276	liquid environments	258:276	liquid environments	258:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	10	4	theme	STUDY	1545:1549	arg1	N/A					1551:1553	STUDY N/A	1545:1553	STUDY N/A	1545:1553	TYPE OF STUDY N/A (animal and laboratory study).					
33109345	11	5	theme	laboratory	1620:1629	arg1	N/A					1604:1606	N/A	1604:1606	N/A	1604:1606	LEVEL OF EVIDENCE N/A (animal and laboratory study).					
33109345	11	5	theme	laboratory	1620:1629	arg1	study					1631:1635	animal and laboratory study	1609:1635	animal and laboratory study	1609:1635	LEVEL OF EVIDENCE N/A (animal and laboratory study).					
33109345	6	6	theme	Adherent	884:891	arg1	hydrogels					893:901	Adherent hydrogels	884:901	Adherent hydrogels	884:901	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	8	7	theme	selective	1225:1233	arg1	attachment					1243:1252	selective, stable attachment	1225:1252	selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model	1225:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	11	8	theme	animal	1609:1614	arg1	N/A					1604:1606	N/A	1604:1606	N/A	1604:1606	LEVEL OF EVIDENCE N/A (animal and laboratory study).					
33109345	11	8	theme	animal	1609:1614	arg1	study					1631:1635	animal and laboratory study	1609:1635	animal and laboratory study	1609:1635	LEVEL OF EVIDENCE N/A (animal and laboratory study).					
33109345	2	9	theme	METHODS	279:285	arg1	fetuses					294:300	METHODS Rabbit fetuses	279:300	METHODS Rabbit fetuses (n = 23)	279:309	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	2	9	theme	METHODS	279:285	arg1	n = 23					303:308	n = 23	303:308	n = 23	303:308	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	1	10	theme	novel	173:177	arg1	composite					203:211	a novel, expandable bioadhesive composite	171:211	a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments	171:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	9	11	theme	valuable	1435:1442	arg1	alternative					1444:1454	a valuable alternative	1433:1454	a valuable alternative for the prenatal repair of spina bifida	1433:1494	It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.					
33109345	7	12	theme	repair	1066:1071	arg1	failure					1073:1079	repair failure	1066:1079	repair failure	1066:1079	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	10	13	dep	TYPE	1537:1540	arg1	study					1578:1582	animal and laboratory study	1556:1582	TYPE OF STUDY N/A (animal and laboratory study).	1537:1584	TYPE OF STUDY N/A (animal and laboratory study).					
33109345	3	14	theme	crosslinked	567:577	arg1	polyacrylamide					579:592	covalently crosslinked polyacrylamide	556:592	covalently crosslinked polyacrylamide	556:592	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	5	15	theme	last	836:839	arg1	fetuses					844:850	the last 14 fetuses	832:850	the last 14 fetuses	832:850	RESULTS Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized.					
33109345	8	16	theme	bioadhesive	1192:1202	arg1	composite					1204:1212	This novel bioadhesive composite	1181:1212	This novel bioadhesive composite	1181:1212	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	8	17	theme	alginate-polyacrylamide	1260:1282	arg1	hydrogel					1284:1291	an alginate-polyacrylamide hydrogel	1257:1291	an alginate-polyacrylamide hydrogel	1257:1291	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	7	18	theme	median	1039:1044	arg1	15 mmHg					1085:1091	15 mmHg	1085:1091	15 mmHg	1085:1091	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	7	18	theme	median	1039:1044	arg1	pressure					1054:1061	The median maximum pressure	1035:1061	The median maximum pressure to repair failure	1035:1079	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	1	19	from	control	247:253	arg1	environments					265:276	liquid environments	258:276	liquid environments	258:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	9	20	theme	prenatal	1464:1471	arg1	repair					1473:1478	the prenatal repair	1460:1478	the prenatal repair of spina bifida	1460:1494	It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.					
33109345	0	21	theme	two-component	8:20	arg1	expandable					23:32	A novel two-component, expandable	0:32	expandable	23:32	A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.					
33109345	9	22	theme	bifida	1489:1494	arg1	repair					1473:1478	the prenatal repair	1460:1478	the prenatal repair of spina bifida	1460:1494	It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.					
33109345	6	23	theme	defect	980:985	arg1	coverage					987:994	defect coverage	980:994	defect coverage confirmed by ultrasound and histology	980:1032	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	4	24	theme	pressure	724:731	arg1	testing					733:739	hydraulic pressure testing	714:739	hydraulic pressure testing	714:739	Animals were euthanized prior to term for different analyses, including hydraulic pressure testing.					
33109345	8	25	from	areas	1305:1309	arg1	model					1356:1360	a fetal rabbit model	1341:1360	a fetal rabbit model	1341:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	9	26	theme	congenital	1515:1524	arg1	anomalies					1526:1534	other congenital anomalies	1509:1534	other congenital anomalies	1509:1534	It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.					
33109345	3	27	attach	linked	594:599	arg1	chitosan					615:622	a bridging chitosan	604:622	a bridging chitosan polymer adhesive	604:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	3	27	attach	linked	594:599	arg2	alginate					543:550	ionically crosslinked alginate	521:550	ionically crosslinked alginate	521:550	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	6	28	from	euthanasia	963:972	arg1	increase					943:950	a median two-fold (IQR: 1.7-2.4) increase	910:950	a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia	910:972	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	6	28	from	euthanasia	963:972	arg1	area					955:958	area	955:958	area at euthanasia	955:972	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	8	29	theme	fetal	1343:1347	arg1	model					1356:1360	a fetal rabbit model	1341:1360	a fetal rabbit model	1341:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	2	30	theme	term	381:384	arg1	32-33 days					386:395	term 32-33 days	381:395	term 32-33 days	381:395	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	2	30	theme	term	381:384	arg1	day					370:372	gestational day 22-25	358:378	gestational day 22-25 (term 32-33 days)	358:396	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	7	31	theme	fluid	1152:1156	arg1	pressures					1158:1166	reported neonatal cerebrospinal fluid pressures	1120:1166	reported neonatal cerebrospinal fluid pressures	1120:1166	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	6	32	from	increase	943:950	arg1	euthanasia					963:972	euthanasia	963:972	euthanasia	963:972	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	6	32	from	increase	943:950	arg1	area					955:958	area	955:958	area at euthanasia	955:972	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	7	33	theme	neonatal	1129:1136	arg1	fluid					1152:1156	neonatal cerebrospinal fluid	1129:1156	reported neonatal cerebrospinal fluid pressures	1120:1166	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	11	34	dep	N/A	1604:1606	arg1	LEVEL					1586:1590	LEVEL	1586:1590	LEVEL OF EVIDENCE	1586:1602	LEVEL OF EVIDENCE N/A (animal and laboratory study).					
33109345	8	35	theme	hydrogel	1373:1380	arg1	expands					1382:1388	the hydrogel expands	1369:1388	the hydrogel expands with the defect over time	1369:1414	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	2	36	theme	bifida	348:353	arg1	creation					330:337	surgical creation	321:337	surgical creation of spina bifida	321:353	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	5	37	theme	fetuses	810:816	arg1	fetuses					810:816	the recovered fetuses	796:816	the recovered fetuses	796:816	RESULTS Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized.					
33109345	5	37	theme	fetuses	810:816	arg1	16/23					786:790	16/23	786:790	16/23	786:790	RESULTS Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized.					
33109345	5	37	theme	fetuses	810:816	arg1	%					783:783	70%	781:783	70% (16/23) of the recovered fetuses	781:816	RESULTS Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized.					
33109345	3	38	theme	adhesive	632:639	arg1	chitosan					615:622	a bridging chitosan	604:622	a bridging chitosan polymer adhesive	604:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	3	39	theme	crosslinked	531:541	arg1	alginate					543:550	ionically crosslinked alginate	521:550	ionically crosslinked alginate	521:550	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	1	40	theme	select	150:155	arg1	properties					157:166	select properties	150:166	select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments	150:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	8	41	from	defect	1331:1336	arg1	model					1356:1360	a fetal rabbit model	1341:1360	a fetal rabbit model	1341:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	1	42	theme	adhesion	238:245	arg1	control					247:253	enhanced adhesion control	229:253	enhanced adhesion control in liquid environments	229:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	0	43	theme	exposed	50:56	arg1	defect					58:63	exposed defect	50:63	exposed defect	50:63	A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.					
33109345	6	44	theme	two-fold	919:926	arg1	increase					943:950	a median two-fold (IQR: 1.7-2.4) increase	910:950	a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia	910:972	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	10	45	theme	laboratory	1567:1576	arg1	study					1578:1582	animal and laboratory study	1556:1582	TYPE OF STUDY N/A (animal and laboratory study).	1537:1584	TYPE OF STUDY N/A (animal and laboratory study).					
33109345	2	46	theme	gestational	358:368	arg1	32-33 days					386:395	term 32-33 days	381:395	term 32-33 days	381:395	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	2	46	theme	gestational	358:368	arg1	day					370:372	gestational day 22-25	358:378	gestational day 22-25 (term 32-33 days)	358:396	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	10	47	theme	N/A	1551:1553	arg1	TYPE					1537:1540	TYPE	1537:1540	TYPE OF STUDY N/A (animal and laboratory study).	1537:1584	TYPE OF STUDY N/A (animal and laboratory study).					
33109345	11	48	theme	EVIDENCE	1595:1602	arg1	LEVEL					1586:1590	LEVEL	1586:1590	LEVEL OF EVIDENCE	1586:1602	LEVEL OF EVIDENCE N/A (animal and laboratory study).					
33109345	3	49	theme	alginate	543:550	arg1	network					510:516	a double network	501:516	a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive	501:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	3	50	theme	polyacrylamide	579:592	arg1	network					510:516	a double network	501:516	a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive	501:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	8	51	theme	bifida	1324:1329	arg1	defect					1331:1336	the spina bifida defect	1314:1336	the spina bifida defect in a fetal rabbit model	1314:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	8	52	attach	attachment	1243:1252	arg2	hydrogel					1284:1291	an alginate-polyacrylamide hydrogel	1257:1291	an alginate-polyacrylamide hydrogel	1257:1291	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	8	52	attach	attachment	1243:1252	arg1	areas					1305:1309	specific areas	1296:1309	specific areas of the spina bifida defect in a fetal rabbit model	1296:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	2	53	theme	Rabbit	287:292	arg1	fetuses					294:300	METHODS Rabbit fetuses	279:300	METHODS Rabbit fetuses (n = 23)	279:309	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	2	53	theme	Rabbit	287:292	arg1	n = 23					303:308	n = 23	303:308	n = 23	303:308	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	7	54	dep	15 mmHg	1085:1091	arg1	IQR					1094:1096	IQR	1094:1096	IQR: 7.8-55.3	1094:1106	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	2	55	theme	surgical	321:328	arg1	creation					330:337	surgical creation	321:337	surgical creation of spina bifida	321:353	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	4	56	theme	different	684:692	arg1	testing					733:739	hydraulic pressure testing	714:739	hydraulic pressure testing	714:739	Animals were euthanized prior to term for different analyses, including hydraulic pressure testing.					
33109345	4	56	theme	different	684:692	arg1	analyses					694:701	different analyses	684:701	different analyses	684:701	Animals were euthanized prior to term for different analyses, including hydraulic pressure testing.					
33109345	7	57	dep	IQR	1094:1096	arg1	7.8-55.3					1099:1106	7.8-55.3	1099:1106	7.8-55.3	1099:1106	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	8	58	with	expands	1382:1388	arg1	defect					1399:1404	the defect	1395:1404	the defect over time	1395:1414	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	7	59	theme	maximum	1046:1052	arg1	15 mmHg					1085:1091	15 mmHg	1085:1091	15 mmHg	1085:1091	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	7	59	theme	maximum	1046:1052	arg1	pressure					1054:1061	The median maximum pressure	1035:1061	The median maximum pressure to repair failure	1035:1079	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	1	60	theme	bioadhesive	191:201	arg1	composite					203:211	a novel, expandable bioadhesive composite	171:211	a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments	171:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	6	61	dep	IQR	929:931	arg1	1.7-2.4					934:940	1.7-2.4	934:940	1.7-2.4	934:940	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	8	62	theme	spina	1318:1322	arg1	bifida					1324:1329	the spina bifida	1314:1329	the spina bifida defect in a fetal rabbit model	1314:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	5	63	theme	RESULTS	742:748	arg1	Hydrogels					750:758	RESULTS Hydrogels	742:758	RESULTS Hydrogels	742:758	RESULTS Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized.					
33109345	3	64	theme	bridging	606:613	arg1	chitosan					615:622	a bridging chitosan	604:622	a bridging chitosan polymer adhesive	604:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	8	65	theme	novel	1186:1190	arg1	composite					1204:1212	This novel bioadhesive composite	1181:1212	This novel bioadhesive composite	1181:1212	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	10	66	theme	animal	1556:1561	arg1	study					1578:1582	animal and laboratory study	1556:1582	TYPE OF STUDY N/A (animal and laboratory study).	1537:1584	TYPE OF STUDY N/A (animal and laboratory study).					
33109345	6	67	dep	increase	943:950	arg1	IQR					929:931	IQR	929:931	IQR: 1.7-2.4	929:940	Adherent hydrogels showed a median two-fold (IQR: 1.7-2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology.					
33109345	8	68	theme	hydrogel	1284:1291	arg1	attachment					1243:1252	selective, stable attachment	1225:1252	selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model	1225:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	8	69	theme	specific	1296:1303	arg1	areas					1305:1309	specific areas	1296:1309	specific areas of the spina bifida defect in a fetal rabbit model	1296:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	9	70	theme	spina	1483:1487	arg1	bifida					1489:1494	spina bifida	1483:1494	spina bifida	1483:1494	It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.					
33109345	3	71	link	crosslinked	567:577	arg1	polyacrylamide					579:592	covalently crosslinked polyacrylamide	556:592	covalently crosslinked polyacrylamide	556:592	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	4	72	theme	hydraulic	714:722	arg1	testing					733:739	hydraulic pressure testing	714:739	hydraulic pressure testing	714:739	Animals were euthanized prior to term for different analyses, including hydraulic pressure testing.					
33109345	0	73	theme	prenatal	92:99	arg1	procedures					101:110	prenatal procedures	92:110	prenatal procedures	92:110	A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.					
33109345	9	74	theme	other	1509:1513	arg1	anomalies					1526:1534	other congenital anomalies	1509:1534	other congenital anomalies	1509:1534	It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.					
33109345	3	75	link	crosslinked	531:541	arg1	alginate					543:550	ionically crosslinked alginate	521:550	ionically crosslinked alginate	521:550	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	8	76	theme	defect	1331:1336	arg1	areas					1305:1309	specific areas	1296:1309	specific areas of the spina bifida defect in a fetal rabbit model	1296:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	1	77	theme	composite	203:211	arg1	properties					157:166	select properties	150:166	select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments	150:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	3	78	theme	two-component	439:451	arg1	tough					453:457	a two-component tough adhesive	437:466	a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive	437:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	0	79	theme	novel	2:6	arg1	expandable					23:32	A novel two-component, expandable	0:32	expandable	23:32	A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.					
33109345	8	80	theme	rabbit	1349:1354	arg1	model					1356:1360	a fetal rabbit model	1341:1360	a fetal rabbit model	1341:1360	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	7	81	theme	reported	1120:1127	arg1	pressures					1158:1166	reported neonatal cerebrospinal fluid pressures	1120:1166	reported neonatal cerebrospinal fluid pressures	1120:1166	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
33109345	8	82	dep	selective	1225:1233	arg1	stable					1236:1241	stable	1236:1241	stable	1236:1241	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	0	83	theme	bioadhesive	34:44	arg1	expandable					23:32	A novel two-component, expandable	0:32	expandable	23:32	A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.					
33109345	3	84	theme	adhesive	459:466	arg1	tough					453:457	a two-component tough adhesive	437:466	a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive	437:639	Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive.					
33109345	5	85	theme	recovered	800:808	arg1	fetuses					810:816	the recovered fetuses	796:816	the recovered fetuses	796:816	RESULTS Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized.					
33109345	8	86	dep	CONCLUSIONS	1169:1179	arg1	allows					1214:1219	allows	1214:1219	allows	1214:1219	CONCLUSIONS This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time.					
33109345	1	87	dep	novel	173:177	arg1	expandable					180:189	expandable	180:189	expandable	180:189	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	2	88	theme	spina	342:346	arg1	bifida					348:353	spina bifida	342:353	spina bifida	342:353	METHODS Rabbit fetuses (n = 23) underwent surgical creation of spina bifida on gestational day 22-25 (term 32-33 days).					
33109345	1	89	theme	enhanced	229:236	arg1	control					247:253	enhanced adhesion control	229:253	enhanced adhesion control in liquid environments	229:276	BACKGROUND/PURPOSE We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments.					
33109345	7	90	theme	cerebrospinal	1138:1150	arg1	fluid					1152:1156	neonatal cerebrospinal fluid	1129:1156	reported neonatal cerebrospinal fluid pressures	1120:1166	The median maximum pressure to repair failure was 15 mmHg (IQR: 7.8-55.3), exceeding reported neonatal cerebrospinal fluid pressures.					
32828977	0	0	theme	improved	109:116	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	0	theme	improved	109:116	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	3	1	theme	nanofibers	357:366	arg1	appropriate					371:381	appropriate	371:381	appropriate	371:381	The combination of PACT and electrospinning nanofibers is appropriate for wound healing, especially infected wound.					
32828977	3	1	theme	nanofibers	357:366	arg1	combination					317:327	The combination	313:327	The combination of PACT and electrospinning nanofibers	313:366	The combination of PACT and electrospinning nanofibers is appropriate for wound healing, especially infected wound.					
32828977	5	2	theme	%	714:714	arg1	chitosan					722:729	2% (w/v) chitosan	713:729	2% (w/v) chitosan	713:729	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	5	3	theme	%	736:736	arg1	PVA					744:746	7% (w/v) PVA	735:746	7% (w/v) PVA	735:746	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	8	4	theme	nanofibers	1194:1203	arg1	surface					1179:1185	the surface	1175:1185	the surface of the nanofibers	1175:1203	ICG released rapidly from the surface of the nanofibers first and then released continuously.					
32828977	4	5	theme	antibiotic-resistant	524:543	arg1	bacteria					545:552	two antibiotic-resistant bacteria	520:552	two antibiotic-resistant bacteria	520:552	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	4	5	theme	antibiotic-resistant	524:543	arg1	aeruginosa					642:651	Pseudomonas aeruginosa	630:651	Meropenem-resistant Pseudomonas aeruginosa (MRPA)	610:658	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	4	5	theme	antibiotic-resistant	524:543	arg1	aureus					592:597	Staphylococcus aureus	577:597	Staphylococcus aureus (MRSA)	577:604	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	11	6	theme	promising	1668:1676	arg1	option					1678:1683	a novel promising option	1660:1683	a novel promising option for treatment of infected wound	1660:1715	The photosensitizer-loaded electrospinning nanofibers provide a novel promising option for treatment of infected wound.					
32828977	9	7	theme	photodynamic	1247:1258	arg1	nanofibers					1260:1269	The photodynamic nanofibers	1243:1269	The photodynamic nanofibers	1243:1269	The photodynamic nanofibers could inhibit the bacteria and decreased the F4/80 expression of MRSA-infected rats.					
32828977	10	8	theme	necrosis	1546:1553	arg1	factor-α					1555:1562	tumor necrosis factor-α	1540:1562	tumor necrosis factor-α (TNF-α)	1540:1570	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	8	theme	necrosis	1546:1553	arg1	TNF-α					1565:1569	TNF-α	1565:1569	TNF-α	1565:1569	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	9	theme	differentiation	1490:1504	arg1	CD31					1510:1513	CD31	1510:1513	CD31	1510:1513	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	9	theme	differentiation	1490:1504	arg1	cluster					1479:1485	cluster	1479:1485	cluster of differentiation 31 (CD31)	1479:1514	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	5	10	theme	optimized	665:673	arg1	solution					691:698	The optimized electrospinning solution	661:698	The optimized electrospinning solution	661:698	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	11	11	theme	novel	1662:1666	arg1	option					1678:1683	a novel promising option	1660:1683	a novel promising option for treatment of infected wound	1660:1715	The photosensitizer-loaded electrospinning nanofibers provide a novel promising option for treatment of infected wound.					
32828977	5	12	dep	%	736:736	arg1	w/v					739:741	w/v	739:741	w/v	739:741	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	1	13	contain	has	193:195	arg2	resistance					233:242	low resistance	229:242	low resistance to drugs	229:251	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	13	contain	has	193:195	arg2	advantages					197:206	advantages	197:206	advantages of strong targeting	197:226	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	13	contain	has	193:195	arg1	chemotherapy					173:184	Photodynamic antimicrobial chemotherapy	146:184	Photodynamic antimicrobial chemotherapy (PACT)	146:191	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	13	contain	has	193:195	arg1	PACT					187:190	PACT	187:190	PACT	187:190	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	4	14	theme	indocyanine	443:453	arg1	ICG					462:464	ICG	462:464	ICG	462:464	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	4	14	theme	indocyanine	443:453	arg1	green					455:459	indocyanine green	443:459	indocyanine green (ICG) as photosensitizer	443:484	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	3	15	theme	wound	387:391	arg1	healing					393:399	wound healing	387:399	wound healing	387:399	The combination of PACT and electrospinning nanofibers is appropriate for wound healing, especially infected wound.					
32828977	4	16	theme	inhibition	498:507	arg1	effects					509:515	obvious inhibition effects	490:515	obvious inhibition effects	490:515	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	7	17	theme	nanofibers	1137:1146	arg1	formation					1124:1132	the formation	1120:1132	the formation of nanofibers	1120:1146	Fourier transform infrared spectrum and X-ray diffraction showed that the intermolecular hydrogen bonding happened between chitosan and PVA in electrospinning process, which was favorable for the formation of nanofibers.					
32828977	5	18	theme	electrospinning	675:689	arg1	solution					691:698	The optimized electrospinning solution	661:698	The optimized electrospinning solution	661:698	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	6	19	dep	showed	805:810	arg1	%					925:925	up to 210%	916:925	up to 210%	916:925	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	0	20	theme	infected	129:136	arg1	wounds					138:143	infected wounds	129:143	infected wounds	129:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	11	21	theme	wound	1711:1715	arg1	treatment					1689:1697	treatment	1689:1697	treatment of infected wound	1689:1715	The photosensitizer-loaded electrospinning nanofibers provide a novel promising option for treatment of infected wound.					
32828977	7	22	theme	X-ray	968:972	arg1	diffraction					974:984	X-ray diffraction	968:984	X-ray diffraction	968:984	Fourier transform infrared spectrum and X-ray diffraction showed that the intermolecular hydrogen bonding happened between chitosan and PVA in electrospinning process, which was favorable for the formation of nanofibers.					
32828977	0	23	theme	wounds	138:143	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	23	theme	wounds	138:143	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	23	theme	wounds	138:143	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	24	theme	photodynamic	11:22	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	24	theme	photodynamic	11:22	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	24	theme	photodynamic	11:22	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	10	25	theme	cluster	1479:1485	arg1	IL-6					1591:1594	IL-6	1591:1594	IL-6	1591:1594	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	25	theme	cluster	1479:1485	arg1	morphology					1418:1427	the morphology	1414:1427	the morphology	1414:1427	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	25	theme	cluster	1479:1485	arg1	level					1531:1535	the decreased level	1517:1535	the decreased level of tumor necrosis factor-α (TNF-α)	1517:1570	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	25	theme	cluster	1479:1485	arg1	expression					1465:1474	the increased expression	1451:1474	the increased expression of cluster of differentiation 31 (CD31)	1451:1514	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	25	theme	cluster	1479:1485	arg1	interleukin-6					1576:1588	interleukin-6	1576:1588	interleukin-6 (IL-6)	1576:1595	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	25	theme	cluster	1479:1485	arg1	radio					1444:1448	wound healing radio	1430:1448	wound healing radio	1430:1448	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	6	26	theme	three-dimensional	814:830	arg1	ratio					888:892	the water absorption ratio	867:892	the water absorption ratio of the nanofibers	867:910	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	6	26	theme	three-dimensional	814:830	arg1	cross-network					832:844	a three-dimensional cross-network	812:844	a three-dimensional cross-network with smooth surface	812:864	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	11	27	theme	electrospinning	1625:1639	arg1	nanofibers					1641:1650	The photosensitizer-loaded electrospinning nanofibers	1598:1650	The photosensitizer-loaded electrospinning nanofibers	1598:1650	The photosensitizer-loaded electrospinning nanofibers provide a novel promising option for treatment of infected wound.					
32828977	1	28	theme	strong	211:216	arg1	targeting					218:226	strong targeting	211:226	strong targeting	211:226	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	0	29	theme	ICG-loaded	0:9	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	29	theme	ICG-loaded	0:9	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	29	theme	ICG-loaded	0:9	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	5	30	theme	2	713:713	arg1	%					714:714	%	714:714	%	714:714	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	10	31	theme	wound	1380:1384	arg1	healing					1386:1392	wound healing	1380:1392	wound healing	1380:1392	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	1	32	theme	targeting	218:226	arg1	advantages					197:206	advantages	197:206	advantages of strong targeting	197:226	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	32	theme	targeting	218:226	arg1	resistance					233:242	low resistance	229:242	low resistance to drugs	229:251	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	0	33	theme	alcohol	43:49	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	33	theme	alcohol	43:49	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	33	theme	alcohol	43:49	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	10	34	theme	tumor	1540:1544	arg1	factor-α					1555:1562	tumor necrosis factor-α	1540:1562	tumor necrosis factor-α (TNF-α)	1540:1570	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	34	theme	tumor	1540:1544	arg1	TNF-α					1565:1569	TNF-α	1565:1569	TNF-α	1565:1569	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	35	theme	healing	1436:1442	arg1	radio					1444:1448	wound healing radio	1430:1448	wound healing radio	1430:1448	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	7	36	dep	Fourier	928:934	arg1	transform					936:944	transform	936:944	transform infrared spectrum and X-ray diffraction	936:984	Fourier transform infrared spectrum and X-ray diffraction showed that the intermolecular hydrogen bonding happened between chitosan and PVA in electrospinning process, which was favorable for the formation of nanofibers.					
32828977	4	37	contain	had	486:488	arg1	ICG					462:464	ICG	462:464	ICG	462:464	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	4	37	contain	had	486:488	arg1	green					455:459	indocyanine green	443:459	indocyanine green (ICG) as photosensitizer	443:484	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	4	37	contain	had	486:488	arg2	effects					509:515	obvious inhibition effects	490:515	obvious inhibition effects	490:515	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	0	38	theme	chitosan/polyvinyl	24:41	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	38	theme	chitosan/polyvinyl	24:41	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	38	theme	chitosan/polyvinyl	24:41	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	11	39	theme	photosensitizer-loaded	1602:1623	arg1	nanofibers					1641:1650	The photosensitizer-loaded electrospinning nanofibers	1598:1650	The photosensitizer-loaded electrospinning nanofibers	1598:1650	The photosensitizer-loaded electrospinning nanofibers provide a novel promising option for treatment of infected wound.					
32828977	1	40	theme	low	229:231	arg1	advantages					197:206	advantages	197:206	advantages of strong targeting	197:226	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	40	theme	low	229:231	arg1	resistance					233:242	low resistance	229:242	low resistance to drugs	229:251	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	6	41	theme	nanofibers	901:910	arg1	ratio					888:892	the water absorption ratio	867:892	the water absorption ratio of the nanofibers	867:910	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	6	41	theme	nanofibers	901:910	arg1	cross-network					832:844	a three-dimensional cross-network	812:844	a three-dimensional cross-network with smooth surface	812:864	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	9	42	theme	rats	1350:1353	arg1	expression					1322:1331	the F4/80 expression	1312:1331	the F4/80 expression of MRSA-infected rats	1312:1353	The photodynamic nanofibers could inhibit the bacteria and decreased the F4/80 expression of MRSA-infected rats.					
32828977	10	43	theme	improved	1360:1367	arg1	effects					1369:1375	The improved effects	1356:1375	The improved effects of wound healing	1356:1392	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	0	44	theme	composite	51:59	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	44	theme	composite	51:59	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	44	theme	composite	51:59	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	10	45	theme	decreased	1521:1529	arg1	level					1531:1535	the decreased level	1517:1535	the decreased level of tumor necrosis factor-α (TNF-α)	1517:1570	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	4	46	theme	obvious	490:496	arg1	effects					509:515	obvious inhibition effects	490:515	obvious inhibition effects	490:515	In our study, indocyanine green (ICG) as photosensitizer had obvious inhibition effects on two antibiotic-resistant bacteria, Methicillin-resistant Staphylococcus aureus (MRSA) and Meropenem-resistant Pseudomonas aeruginosa (MRPA).					
32828977	2	47	theme	Electrospinning	254:268	arg1	nanofibers					270:279	Electrospinning nanofibers	254:279	Electrospinning nanofibers	254:279	Electrospinning nanofibers is favorable for wound healing.					
32828977	0	48	theme	Anti-resistant	73:86	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	48	theme	Anti-resistant	73:86	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	6	49	theme	smooth	851:856	arg1	surface					858:864	smooth surface	851:864	smooth surface	851:864	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	0	50	dep	nanofibers	61:70	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	50	dep	nanofibers	61:70	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	50	dep	nanofibers	61:70	arg1	healing					118:124	improved healing	109:124	improved healing of infected wounds	109:143	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	6	51	theme	electron	785:792	arg1	microscope					794:803	scanning electron microscope	776:803	scanning electron microscope	776:803	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	3	52	theme	electrospinning	341:355	arg1	nanofibers					357:366	PACT and electrospinning nanofibers	332:366	PACT and electrospinning nanofibers	332:366	The combination of PACT and electrospinning nanofibers is appropriate for wound healing, especially infected wound.					
32828977	9	53	theme	F4/80	1316:1320	arg1	expression					1322:1331	the F4/80 expression	1312:1331	the F4/80 expression of MRSA-infected rats	1312:1353	The photodynamic nanofibers could inhibit the bacteria and decreased the F4/80 expression of MRSA-infected rats.					
32828977	6	54	theme	scanning	776:783	arg1	microscope					794:803	scanning electron microscope	776:803	scanning electron microscope	776:803	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	1	55	theme	Photodynamic	146:157	arg1	PACT					187:190	PACT	187:190	PACT	187:190	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	55	theme	Photodynamic	146:157	arg1	chemotherapy					173:184	Photodynamic antimicrobial chemotherapy	146:184	Photodynamic antimicrobial chemotherapy (PACT)	146:191	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	10	56	theme	increased	1455:1463	arg1	expression					1465:1474	the increased expression	1451:1474	the increased expression of cluster of differentiation 31 (CD31)	1451:1514	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	5	57	theme	7	735:735	arg1	%					736:736	%	736:736	%	736:736	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	6	58	theme	absorption	877:886	arg1	ratio					888:892	the water absorption ratio	867:892	the water absorption ratio of the nanofibers	867:910	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	6	58	theme	absorption	877:886	arg1	cross-network					832:844	a three-dimensional cross-network	812:844	a three-dimensional cross-network with smooth surface	812:864	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	2	59	theme	wound	298:302	arg1	healing					304:310	wound healing	298:310	wound healing	298:310	Electrospinning nanofibers is favorable for wound healing.					
32828977	7	60	theme	intermolecular	1002:1015	arg1	bonding					1026:1032	the intermolecular hydrogen bonding	998:1032	the intermolecular hydrogen bonding	998:1032	Fourier transform infrared spectrum and X-ray diffraction showed that the intermolecular hydrogen bonding happened between chitosan and PVA in electrospinning process, which was favorable for the formation of nanofibers.					
32828977	1	61	theme	antimicrobial	159:171	arg1	PACT					187:190	PACT	187:190	PACT	187:190	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	1	61	theme	antimicrobial	159:171	arg1	chemotherapy					173:184	Photodynamic antimicrobial chemotherapy	146:184	Photodynamic antimicrobial chemotherapy (PACT)	146:191	Photodynamic antimicrobial chemotherapy (PACT) has advantages of strong targeting, low resistance to drugs.					
32828977	7	62	theme	infrared	946:953	arg1	spectrum					955:962	infrared spectrum	946:962	infrared spectrum	946:962	Fourier transform infrared spectrum and X-ray diffraction showed that the intermolecular hydrogen bonding happened between chitosan and PVA in electrospinning process, which was favorable for the formation of nanofibers.					
32828977	5	63	dep	%	714:714	arg1	w/v					717:719	w/v	717:719	w/v	717:719	The optimized electrospinning solution consisted of 2% (w/v) chitosan and 7% (w/v) PVA.					
32828977	3	64	theme	PACT	332:335	arg1	nanofibers					357:366	PACT and electrospinning nanofibers	332:366	PACT and electrospinning nanofibers	332:366	The combination of PACT and electrospinning nanofibers is appropriate for wound healing, especially infected wound.					
32828977	6	65	theme	water	871:875	arg1	absorption					877:886	water absorption	871:886	the water absorption ratio of the nanofibers	867:910	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	10	66	theme	wound	1430:1434	arg1	healing					1436:1442	wound healing	1430:1442	wound healing radio	1430:1448	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	11	67	theme	infected	1702:1709	arg1	wound					1711:1715	infected wound	1702:1715	infected wound	1702:1715	The photosensitizer-loaded electrospinning nanofibers provide a novel promising option for treatment of infected wound.					
32828977	10	68	theme	healing	1386:1392	arg1	effects					1369:1375	The improved effects	1356:1375	The improved effects of wound healing	1356:1392	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	69	theme	factor-α	1555:1562	arg1	IL-6					1591:1594	IL-6	1591:1594	IL-6	1591:1594	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	69	theme	factor-α	1555:1562	arg1	morphology					1418:1427	the morphology	1414:1427	the morphology	1414:1427	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	69	theme	factor-α	1555:1562	arg1	level					1531:1535	the decreased level	1517:1535	the decreased level of tumor necrosis factor-α (TNF-α)	1517:1570	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	69	theme	factor-α	1555:1562	arg1	expression					1465:1474	the increased expression	1451:1474	the increased expression of cluster of differentiation 31 (CD31)	1451:1514	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	69	theme	factor-α	1555:1562	arg1	interleukin-6					1576:1588	interleukin-6	1576:1588	interleukin-6 (IL-6)	1576:1595	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	10	69	theme	factor-α	1555:1562	arg1	radio					1444:1448	wound healing radio	1430:1448	wound healing radio	1430:1448	The improved effects of wound healing were evaluated with the morphology, wound healing radio, the increased expression of cluster of differentiation 31 (CD31), the decreased level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).					
32828977	6	70	with	cross-network	832:844	arg1	surface					858:864	smooth surface	851:864	smooth surface	851:864	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32828977	0	71	theme	bacterial	88:96	arg1	nanofibers					61:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers	0:70	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.	0:144	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	0	71	theme	bacterial	88:96	arg1	effect					98:103	Anti-resistant bacterial effect	73:103	Anti-resistant bacterial effect	73:103	ICG-loaded photodynamic chitosan/polyvinyl alcohol composite nanofibers: Anti-resistant bacterial effect and improved healing of infected wounds.					
32828977	9	72	theme	MRSA-infected	1336:1348	arg1	rats					1350:1353	MRSA-infected rats	1336:1353	MRSA-infected rats	1336:1353	The photodynamic nanofibers could inhibit the bacteria and decreased the F4/80 expression of MRSA-infected rats.					
32828977	7	73	theme	hydrogen	1017:1024	arg1	bonding					1026:1032	the intermolecular hydrogen bonding	998:1032	the intermolecular hydrogen bonding	998:1032	Fourier transform infrared spectrum and X-ray diffraction showed that the intermolecular hydrogen bonding happened between chitosan and PVA in electrospinning process, which was favorable for the formation of nanofibers.					
32828977	8	74	attach	released	1153:1160	arg2	ICG					1149:1151	ICG	1149:1151	ICG released rapidly from the surface of the nanofibers	1149:1203	ICG released rapidly from the surface of the nanofibers first and then released continuously.					
32828977	8	74	attach	released	1153:1160	arg1	surface					1179:1185	the surface	1175:1185	the surface of the nanofibers	1175:1203	ICG released rapidly from the surface of the nanofibers first and then released continuously.					
32828977	6	75	dep	210	922:924	arg1	to					919:920	to	919:920	to	919:920	The nanofibers observed by scanning electron microscope showed a three-dimensional cross-network with smooth surface, the water absorption ratio of the nanofibers was up to 210%.					
32553963	8	0	theme	Scanning	1222:1229	arg1	Microscope					1240:1249	Scanning Electron Microscope	1222:1249	Scanning Electron Microscope (SEM) analysis	1222:1264	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	8	0	theme	Scanning	1222:1229	arg1	SEM					1252:1254	SEM	1252:1254	SEM	1252:1254	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	0	1	theme	fabrics	199:205	arg1	properties					169:178	the antibacterial properties	151:178	improvement the antibacterial properties of cellulose based fabrics	139:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	7	2	theme	Gram-negative	1143:1155	arg1	bacteria					1157:1164	both Gram-positive and Gram-negative bacteria	1120:1164	both Gram-positive and Gram-negative bacteria	1120:1164	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	6	3	theme	antibacterial	950:962	arg1	activity					964:971	antibacterial activity	950:971	antibacterial activity	950:971	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	0	4	theme	based	193:197	arg1	fabrics					199:205	cellulose based fabrics	183:205	cellulose based fabrics	183:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	8	5	theme	chitosan	1291:1298	arg1	nanoparticles					1300:1312	chitosan nanoparticles	1291:1312	chitosan nanoparticles	1291:1312	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	1	6	theme	controlled	258:267	arg1	systems					283:289	controlled drug delivery systems	258:289	controlled drug delivery systems based on nanotechnology	258:313	The main aim of this research work was to develop controlled drug delivery systems based on nanotechnology.					
32553963	0	7	theme	novel	108:112	arg1	system					128:133	novel drug delivery system	108:133	novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics	108:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	7	8	theme	chitosan	1019:1026	arg1	nanoparticles					1028:1040	chitosan nanoparticles	1019:1040	chitosan nanoparticles	1019:1040	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	6	9	theme	50:50	839:843	arg1	fabrics					871:877	(50:50) cotton/polyester blended fabrics	838:877	(50:50) cotton/polyester blended fabrics	838:877	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	1	10	theme	drug	269:272	arg1	systems					283:289	controlled drug delivery systems	258:289	controlled drug delivery systems based on nanotechnology	258:313	The main aim of this research work was to develop controlled drug delivery systems based on nanotechnology.					
32553963	8	11	theme	Electron	1231:1238	arg1	Microscope					1240:1249	Scanning Electron Microscope	1222:1249	Scanning Electron Microscope (SEM) analysis	1222:1264	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	8	11	theme	Electron	1231:1238	arg1	SEM					1252:1254	SEM	1252:1254	SEM	1252:1254	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	7	12	theme	treated	998:1004	arg1	fabrics					1006:1012	the treated fabrics	994:1012	the treated fabrics with chitosan nanoparticles	994:1040	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	1	13	theme	delivery	274:281	arg1	systems					283:289	controlled drug delivery systems	258:289	controlled drug delivery systems based on nanotechnology	258:313	The main aim of this research work was to develop controlled drug delivery systems based on nanotechnology.					
32553963	0	14	theme	delivery	119:126	arg1	system					128:133	novel drug delivery system	108:133	novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics	108:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	6	15	theme	composite	930:938	arg1	concentrations					907:920	different concentrations	897:920	different concentrations of this composite to impart antibacterial activity	897:971	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	3	16	theme	gelation	541:548	arg1	method					550:555	ionic gelation method	535:555	ionic gelation method	535:555	CSNPs were prepared from chitosan solution by using tripolyphosphate (TPP) via ionic gelation method.					
32553963	4	17	from	nanoparticles	698:710	arg1	nanocomposite					652:664	nanocomposite	652:664	nanocomposite from antibiotic loaded chitosan nanoparticles	652:710	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	4	18	theme	chitosan	689:696	arg1	nanoparticles					698:710	antibiotic loaded chitosan nanoparticles	671:710	antibiotic loaded chitosan nanoparticles	671:710	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	0	19	theme	drug	114:117	arg1	system					128:133	novel drug delivery system	108:133	novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics	108:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	2	20	theme	antibiotic	395:404	arg1	ciprofloxacin					441:453	ciprofloxacin	441:453	ciprofloxacin	441:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	20	theme	antibiotic	395:404	arg1	drugs					406:410	the selected antibiotic drugs	382:410	the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin	382:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	20	theme	antibiotic	395:404	arg1	gentamycin					426:435	gentamycin	426:435	gentamycin	426:435	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	20	theme	antibiotic	395:404	arg1	tetracycline					412:423	tetracycline	412:423	tetracycline	412:423	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	7	21	theme	bacteria	1157:1164	arg1	growth					1110:1115	the growth	1106:1115	the growth of both Gram-positive and Gram-negative bacteria	1106:1164	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	2	22	theme	selected	386:393	arg1	ciprofloxacin					441:453	ciprofloxacin	441:453	ciprofloxacin	441:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	22	theme	selected	386:393	arg1	drugs					406:410	the selected antibiotic drugs	382:410	the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin	382:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	22	theme	selected	386:393	arg1	gentamycin					426:435	gentamycin	426:435	gentamycin	426:435	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	22	theme	selected	386:393	arg1	tetracycline					412:423	tetracycline	412:423	tetracycline	412:423	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	4	23	theme	prepared	567:574	arg1	CSNPs					593:597	the prepared high performance CSNPs	563:597	the prepared high performance CSNPs	563:597	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	0	24	theme	chitosan	29:36	arg1	Preparation					0:10	Preparation	0:10	Preparation of biocompatible chitosan	0:36	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	2	25	theme	Chitosan	316:323	arg1	nanocarrier					366:376	a nanocarrier	364:376	a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin	364:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	25	theme	Chitosan	316:323	arg1	CSNPs					340:344	CSNPs	340:344	CSNPs	340:344	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	25	theme	Chitosan	316:323	arg1	nanoparticles					325:337	Chitosan nanoparticles	316:337	Chitosan nanoparticles (CSNPs)	316:345	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	1	26	theme	main	212:215	arg1	aim					217:219	The main aim	208:219	The main aim of this research work	208:241	The main aim of this research work was to develop controlled drug delivery systems based on nanotechnology.					
32553963	0	27	theme	biocompatible	15:27	arg1	chitosan					29:36	biocompatible chitosan	15:36	biocompatible chitosan	15:36	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	4	28	theme	performance	581:591	arg1	CSNPs					593:597	the prepared high performance CSNPs	563:597	the prepared high performance CSNPs	563:597	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	4	29	theme	different	622:630	arg1	antibiotics					632:642	three different antibiotics	616:642	three different antibiotics	616:642	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	8	30	theme	Microscope	1240:1249	arg1	analysis					1257:1264	Scanning Electron Microscope (SEM) analysis	1222:1264	Scanning Electron Microscope (SEM) analysis	1222:1264	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	8	31	dep	Fourier	1171:1177	arg1	Transform					1179:1187	Transform	1179:1187	Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis	1179:1264	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	4	32	theme	high	576:579	arg1	CSNPs					593:597	the prepared high performance CSNPs	563:597	the prepared high performance CSNPs	563:597	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	5	33	theme	superior	753:760	arg1	materials					776:784	superior antibacterial materials	753:784	superior antibacterial materials with minimum toxicity	753:806	Then the prepared nanocomposite used as superior antibacterial materials with minimum toxicity.					
32553963	6	34	theme	different	897:905	arg1	concentrations					907:920	different concentrations	897:920	different concentrations of this composite to impart antibacterial activity	897:971	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	4	35	theme	antibiotic	671:680	arg1	nanoparticles					698:710	antibiotic loaded chitosan nanoparticles	671:710	antibiotic loaded chitosan nanoparticles	671:710	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	5	36	theme	antibacterial	762:774	arg1	materials					776:784	superior antibacterial materials	753:784	superior antibacterial materials with minimum toxicity	753:806	Then the prepared nanocomposite used as superior antibacterial materials with minimum toxicity.					
32553963	6	37	theme	cotton	820:825	arg1	fabrics					871:877	(50:50) cotton/polyester blended fabrics	838:877	(50:50) cotton/polyester blended fabrics	838:877	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	6	37	theme	cotton	820:825	arg1	Samples					809:815	Samples	809:815	Samples of cotton (100%)	809:832	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	8	38	theme	nanoparticles	1300:1312	arg1	embedding					1278:1286	the embedding	1274:1286	the embedding of chitosan nanoparticles	1274:1312	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	7	39	with	fabrics	1006:1012	arg1	nanoparticles					1028:1040	chitosan nanoparticles	1019:1040	chitosan nanoparticles	1019:1040	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	7	39	with	fabrics	1006:1012	arg1	antibiotics					1079:1089	different antibiotics	1069:1089	different antibiotics	1069:1089	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	8	40	theme	Infrared	1189:1196	arg1	FTIR					1212:1215	FTIR	1212:1215	FTIR	1212:1215	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	8	40	theme	Infrared	1189:1196	arg1	spectroscopy					1198:1209	Infrared spectroscopy	1189:1209	Infrared spectroscopy (FTIR)	1189:1216	The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) analysis exposed the embedding of chitosan nanoparticles into fabrics and their antibiotics loaded.					
32553963	5	41	with	materials	776:784	arg1	toxicity					799:806	minimum toxicity	791:806	minimum toxicity	791:806	Then the prepared nanocomposite used as superior antibacterial materials with minimum toxicity.					
32553963	5	42	theme	prepared	722:729	arg1	nanocomposite					731:743	the prepared nanocomposite	718:743	the prepared nanocomposite	718:743	Then the prepared nanocomposite used as superior antibacterial materials with minimum toxicity.					
32553963	7	43	theme	Gram-positive	1125:1137	arg1	bacteria					1157:1164	both Gram-positive and Gram-negative bacteria	1120:1164	both Gram-positive and Gram-negative bacteria	1120:1164	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	5	44	used	used	745:748	arg2	nanocomposite					731:743	the prepared nanocomposite	718:743	the prepared nanocomposite	718:743	Then the prepared nanocomposite used as superior antibacterial materials with minimum toxicity.					
32553963	0	45	theme	antibacterial	155:167	arg1	properties					169:178	the antibacterial properties	151:178	improvement the antibacterial properties of cellulose based fabrics	139:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	0	46	dep	nanoparticles	38:50	arg1	loaded					52:57	loaded	52:57	nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics	38:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	0	47	theme	cellulose	183:191	arg1	fabrics					199:205	cellulose based fabrics	183:205	cellulose based fabrics	183:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	7	48	theme	different	1069:1077	arg1	antibiotics					1079:1089	different antibiotics	1069:1089	different antibiotics	1069:1089	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	1	49	theme	research	229:236	arg1	work					238:241	this research work	224:241	this research work	224:241	The main aim of this research work was to develop controlled drug delivery systems based on nanotechnology.					
32553963	6	50	theme	blended	863:869	arg1	fabrics					871:877	(50:50) cotton/polyester blended fabrics	838:877	(50:50) cotton/polyester blended fabrics	838:877	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	3	51	theme	ionic	535:539	arg1	method					550:555	ionic gelation method	535:555	ionic gelation method	535:555	CSNPs were prepared from chitosan solution by using tripolyphosphate (TPP) via ionic gelation method.					
32553963	1	52	theme	work	238:241	arg1	aim					217:219	The main aim	208:219	The main aim of this research work	208:241	The main aim of this research work was to develop controlled drug delivery systems based on nanotechnology.					
32553963	6	53	theme	cotton/polyester	846:861	arg1	fabrics					871:877	(50:50) cotton/polyester blended fabrics	838:877	(50:50) cotton/polyester blended fabrics	838:877	Samples of cotton (100%) and (50:50) cotton/polyester blended fabrics were treated with different concentrations of this composite to impart antibacterial activity.					
32553963	2	54	dep	drugs	406:410	arg1	ciprofloxacin					441:453	ciprofloxacin	441:453	ciprofloxacin	441:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	54	dep	drugs	406:410	arg1	drugs					406:410	the selected antibiotic drugs	382:410	the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin	382:453	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	54	dep	drugs	406:410	arg1	gentamycin					426:435	gentamycin	426:435	gentamycin	426:435	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	2	54	dep	drugs	406:410	arg1	tetracycline					412:423	tetracycline	412:423	tetracycline	412:423	Chitosan nanoparticles (CSNPs) were selected as a nanocarrier for the selected antibiotic drugs tetracycline, gentamycin and ciprofloxacin.					
32553963	3	55	theme	chitosan	481:488	arg1	solution					490:497	chitosan solution	481:497	chitosan solution	481:497	CSNPs were prepared from chitosan solution by using tripolyphosphate (TPP) via ionic gelation method.					
32553963	4	56	theme	loaded	682:687	arg1	nanoparticles					698:710	antibiotic loaded chitosan nanoparticles	671:710	antibiotic loaded chitosan nanoparticles	671:710	Then the prepared high performance CSNPs were loaded with three different antibiotics to form nanocomposite from antibiotic loaded chitosan nanoparticles.					
32553963	7	57	with	nanocomposite	1050:1062	arg1	nanoparticles					1028:1040	chitosan nanoparticles	1019:1040	chitosan nanoparticles	1019:1040	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	7	57	with	nanocomposite	1050:1062	arg1	antibiotics					1079:1089	different antibiotics	1069:1089	different antibiotics	1069:1089	Results showed that the treated fabrics with chitosan nanoparticles and its nanocomposite with different antibiotics were inhibited the growth of both Gram-positive and Gram-negative bacteria.					
32553963	0	58	dep	improvement	139:149	arg1	properties					169:178	the antibacterial properties	151:178	improvement the antibacterial properties of cellulose based fabrics	139:205	Preparation of biocompatible chitosan nanoparticles loaded by tetracycline, gentamycin and ciprofloxacin as novel drug delivery system for improvement the antibacterial properties of cellulose based fabrics.					
32553963	5	59	theme	minimum	791:797	arg1	toxicity					799:806	minimum toxicity	791:806	minimum toxicity	791:806	Then the prepared nanocomposite used as superior antibacterial materials with minimum toxicity.					
32058191	0	0	theme	rice	75:78	arg1	kernel					80:85	rice kernel	75:85	rice kernel during cooking	75:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	2	1	theme	rice	460:463	arg1	kernel					465:470	rice kernel	460:470	rice kernel	460:470	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	2	2	theme	rheological	317:327	arg1	properties					329:338	the rheological properties	313:338	the rheological properties	313:338	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	1	3	theme	protein	219:225	arg1	effect					209:214	the effect	205:214	the effect of protein on structural properties of rice	205:258	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	1	3	theme	protein	219:225	arg1	controversial					276:288	controversial	276:288	controversial	276:288	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	1	4	theme	important	147:155	arg1	indicators					157:166	important indicators	147:166	important indicators	147:166	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	1	4	theme	important	147:155	arg1	structure					123:131	structure	123:131	structure	123:131	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	1	4	theme	important	147:155	arg1	composition					107:117	composition	107:117	composition	107:117	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	6	5	theme	Fourier	987:993	arg1	results					976:982	The results	972:982	The results of Fourier transform infrared spectra	972:1020	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	5	theme	Fourier	987:993	arg1	analysis					1041:1048	thermogravimetric analysis	1023:1048	thermogravimetric analysis	1023:1048	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	5	theme	Fourier	987:993	arg1	microscope					1076:1085	transmission electron microscope	1054:1085	transmission electron microscope	1054:1085	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	0	6	from	Investigation	0:12	arg1	changes					42:48	molecular and morphology changes	17:48	molecular and morphology changes of protein and starch in rice kernel during cooking	17:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	3	7	theme	overall	515:521	arg1	form					523:526	overall form	515:526	overall form	515:526	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	1	8	theme	structural	230:239	arg1	properties					241:250	structural properties	230:250	structural properties of rice	230:258	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	4	9	theme	molecular	689:697	arg1	mobility					699:706	molecular mobility	689:706	molecular mobility	689:706	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	6	10	theme	surface	1131:1137	arg1	interactions					1139:1150	surface interactions	1131:1150	surface interactions with protein	1131:1163	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	11	with	effect	1110:1115	arg1	protein					1157:1163	protein	1157:1163	protein	1157:1163	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	12	theme	wrapping	1101:1108	arg1	effect					1110:1115	the wrapping effect	1097:1115	the wrapping effect of starch	1097:1125	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	5	13	theme	proton	837:842	arg1	distributions					844:856	The similar proton distributions	825:856	The similar proton distributions between starch, starch-protein mixture and rice flour	825:910	The similar proton distributions between starch, starch-protein mixture and rice flour indicated the weak interactions between starch and protein.					
32058191	0	14	from	changes	42:48	arg1	kernel					80:85	rice kernel	75:85	rice kernel during cooking	75:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	1	15	dep	composition	107:117	arg1	The					103:105	The	103:105	The	103:105	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	2	16	theme	mechanical	418:427	arg1	changes					449:455	mechanical and inner structure changes	418:455	mechanical and inner structure changes of rice kernel during cooking	418:485	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	5	17	theme	weak	926:929	arg1	interactions					931:942	the weak interactions	922:942	the weak interactions between starch and protein	922:969	The similar proton distributions between starch, starch-protein mixture and rice flour indicated the weak interactions between starch and protein.					
32058191	4	18	theme	mobility	699:706	arg1	comparison					675:684	The comparison	671:684	The comparison of molecular mobility in H2O/D2O	671:717	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	2	19	theme	thermal	344:350	arg1	properties					352:361	thermal properties	344:361	thermal properties	344:361	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	2	20	theme	protein	407:413	arg1	effects					396:402	the effects	392:402	the effects of protein on mechanical and inner structure changes of rice kernel during cooking	392:485	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	1	21	theme	rice	255:258	arg1	properties					241:250	structural properties	230:250	structural properties of rice	230:258	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	4	22	theme	protein	801:807	arg1	starch					768:773	starch	768:773	starch	768:773	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	4	22	theme	protein	801:807	arg1	hydrophobicity					783:796	the hydrophobicity	779:796	the hydrophobicity of protein	779:807	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	6	23	theme	electron	1067:1074	arg1	results					976:982	The results	972:982	The results of Fourier transform infrared spectra	972:1020	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	23	theme	electron	1067:1074	arg1	microscope					1076:1085	transmission electron microscope	1054:1085	transmission electron microscope	1054:1085	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	0	24	theme	molecular	17:25	arg1	changes					42:48	molecular and morphology changes	17:48	molecular and morphology changes of protein and starch in rice kernel during cooking	17:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	3	25	theme	inner	532:536	arg1	structure					538:546	inner structure	532:546	inner structure	532:546	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	2	26	theme	inner	433:437	arg1	changes					449:455	mechanical and inner structure changes	418:455	mechanical and inner structure changes of rice kernel during cooking	418:485	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	6	27	theme	transmission	1054:1065	arg1	results					976:982	The results	972:982	The results of Fourier transform infrared spectra	972:1020	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	27	theme	transmission	1054:1065	arg1	microscope					1076:1085	transmission electron microscope	1054:1085	transmission electron microscope	1054:1085	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	1	28	theme	rice	195:198	arg1	quality					184:190	the quality	180:190	the quality of rice	180:198	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	2	29	theme	structure	439:447	arg1	changes					449:455	mechanical and inner structure changes	418:455	mechanical and inner structure changes of rice kernel during cooking	418:485	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	0	30	theme	morphology	31:40	arg1	changes					42:48	molecular and morphology changes	17:48	molecular and morphology changes of protein and starch in rice kernel during cooking	17:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	4	31	theme	hydrophobicity	783:796	arg1	behavior					756:763	the special gelatinization behavior	729:763	the special gelatinization behavior of starch and the hydrophobicity of protein during cooking	729:822	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	5	32	theme	similar	829:835	arg1	distributions					844:856	The similar proton distributions	825:856	The similar proton distributions between starch, starch-protein mixture and rice flour	825:910	The similar proton distributions between starch, starch-protein mixture and rice flour indicated the weak interactions between starch and protein.					
32058191	0	33	theme	protein	53:59	arg1	changes					42:48	molecular and morphology changes	17:48	molecular and morphology changes of protein and starch in rice kernel during cooking	17:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	1	34	from	effect	209:214	arg1	properties					241:250	structural properties	230:250	structural properties of rice	230:258	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	3	35	theme	starch	610:615	arg1	gelatinization					592:605	the limited and uneven gelatinization	569:605	the limited and uneven gelatinization of starch	569:615	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	5	36	theme	rice	901:904	arg1	flour					906:910	rice flour	901:910	rice flour	901:910	The similar proton distributions between starch, starch-protein mixture and rice flour indicated the weak interactions between starch and protein.					
32058191	6	37	dep	Fourier	987:993	arg1	transform					995:1003	transform	995:1003	transform infrared spectra	995:1020	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	38	with	interactions	1139:1150	arg1	protein					1157:1163	protein	1157:1163	protein	1157:1163	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	3	39	theme	good	636:639	arg1	stability					649:657	the good heating stability	632:657	the good heating stability of protein	632:668	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	3	40	theme	kernel	555:560	arg1	form					523:526	overall form	515:526	overall form	515:526	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	3	40	theme	kernel	555:560	arg1	structure					538:546	inner structure	532:546	inner structure	532:546	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	3	41	theme	heating	641:647	arg1	stability					649:657	the good heating stability	632:657	the good heating stability of protein	632:668	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	3	42	theme	uneven	585:590	arg1	gelatinization					592:605	the limited and uneven gelatinization	569:605	the limited and uneven gelatinization of starch	569:615	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	2	43	theme	present	298:304	arg1	study					306:310	the present study	294:310	the present study	294:310	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	6	44	theme	thermogravimetric	1023:1039	arg1	analysis					1041:1048	thermogravimetric analysis	1023:1048	thermogravimetric analysis	1023:1048	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	44	theme	thermogravimetric	1023:1039	arg1	results					976:982	The results	972:982	The results of Fourier transform infrared spectra	972:1020	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	4	45	theme	gelatinization	741:754	arg1	behavior					756:763	the special gelatinization behavior	729:763	the special gelatinization behavior of starch and the hydrophobicity of protein during cooking	729:822	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	4	46	theme	special	733:739	arg1	behavior					756:763	the special gelatinization behavior	729:763	the special gelatinization behavior of starch and the hydrophobicity of protein during cooking	729:822	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	3	47	theme	morphologic	492:502	arg1	changes					504:510	The morphologic changes	488:510	The morphologic changes in overall form and inner structure of the kernel	488:560	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	4	48	from	comparison	675:684	arg1	H2O/D2O					711:717	H2O/D2O	711:717	H2O/D2O	711:717	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	4	49	theme	starch	768:773	arg1	behavior					756:763	the special gelatinization behavior	729:763	the special gelatinization behavior of starch and the hydrophobicity of protein during cooking	729:822	The comparison of molecular mobility in H2O/D2O reflected the special gelatinization behavior of starch and the hydrophobicity of protein during cooking.					
32058191	0	50	theme	starch	65:70	arg1	changes					42:48	molecular and morphology changes	17:48	molecular and morphology changes of protein and starch in rice kernel during cooking	17:100	Investigation on molecular and morphology changes of protein and starch in rice kernel during cooking.					
32058191	6	51	theme	starch	1120:1125	arg1	interactions					1139:1150	surface interactions	1131:1150	surface interactions with protein	1131:1163	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	6	51	theme	starch	1120:1125	arg1	effect					1110:1115	the wrapping effect	1097:1115	the wrapping effect of starch	1097:1125	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
32058191	3	52	theme	limited	573:579	arg1	gelatinization					592:605	the limited and uneven gelatinization	569:605	the limited and uneven gelatinization of starch	569:615	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	5	53	theme	starch-protein	874:887	arg1	mixture					889:895	starch-protein mixture	874:895	starch-protein mixture	874:895	The similar proton distributions between starch, starch-protein mixture and rice flour indicated the weak interactions between starch and protein.					
32058191	2	54	theme	kernel	465:470	arg1	changes					449:455	mechanical and inner structure changes	418:455	mechanical and inner structure changes of rice kernel during cooking	418:485	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	3	55	theme	protein	662:668	arg1	stability					649:657	the good heating stability	632:657	the good heating stability of protein	632:668	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	2	56	from	effects	396:402	arg1	changes					449:455	mechanical and inner structure changes	418:455	mechanical and inner structure changes of rice kernel during cooking	418:485	In the present study, the rheological properties and thermal properties were combined to investigate the effects of protein on mechanical and inner structure changes of rice kernel during cooking.					
32058191	1	57	theme	starch	136:141	arg1	indicators					157:166	important indicators	147:166	important indicators	147:166	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	1	57	theme	starch	136:141	arg1	structure					123:131	structure	123:131	structure	123:131	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	1	57	theme	starch	136:141	arg1	composition					107:117	composition	107:117	composition	107:117	The composition and structure of starch are important indicators to evaluate the quality of rice, but the effect of protein on structural properties of rice has always been controversial.					
32058191	3	58	from	changes	504:510	arg1	form					523:526	overall form	515:526	overall form	515:526	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	3	58	from	changes	504:510	arg1	structure					538:546	inner structure	532:546	inner structure	532:546	The morphologic changes in overall form and inner structure of the kernel showed the limited and uneven gelatinization of starch, and reflected the good heating stability of protein.					
32058191	6	59	theme	infrared	1005:1012	arg1	spectra					1014:1020	infrared spectra	1005:1020	infrared spectra	1005:1020	The results of Fourier transform infrared spectra, thermogravimetric analysis and transmission electron microscope confirmed the wrapping effect of starch and surface interactions with protein.					
33172329	1	0	from	roles	308:312	arg1	function					337:344	intestinal function	326:344	intestinal function	326:344	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	2	1	theme	microbiota	554:563	arg1	activation					500:509	activation	500:509	activation of autophagic flux	500:528	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	2	1	theme	microbiota	554:563	arg1	control					534:540	control	534:540	control of host gut microbiota	534:563	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	5	2	theme	wild-type	1078:1086	arg1	mice					1093:1096	wild-type (WT) mice	1078:1096	wild-type (WT) mice	1078:1096	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	2	3	theme	inflammation	479:490	arg1	amelioration					455:466	amelioration	455:466	amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota	455:563	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	1	4	theme	intestinal	326:335	arg1	function					337:344	intestinal function	326:344	intestinal function	326:344	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	6	5	theme	microbiota	1118:1127	arg1	transplantation					1129:1143	fecal microbiota transplantation	1112:1143	fecal microbiota transplantation	1112:1143	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	1	6	theme	mitochondrial	231:243	arg1	biogenesis					245:254	mitochondrial biogenesis	231:254	mitochondrial biogenesis	231:254	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	0	7	theme	homeostasis	78:88	arg1	regulator					54:62	a critical regulator	43:62	a critical regulator of intestinal homeostasis	43:88	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	0	7	theme	homeostasis	78:88	arg1	ESRRA					0:4	ESRRA	0:4	ESRRA (estrogen related receptor alpha)	0:38	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	7	8	theme	clinical	1400:1407	arg1	relevance					1409:1417	clinical relevance	1400:1417	clinical relevance of ESRRA in UC	1400:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	7	9	from	relevance	1409:1417	arg1	UC					1431:1432	UC	1431:1432	UC	1431:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	7	10	from	expression	1309:1318	arg1	tissues					1342:1348	intestinal mucosal tissues	1323:1348	intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC	1323:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	4	11	theme	protein	778:784	arg1	kinase					786:791	AMP-activated protein kinase	764:791	AMP-activated protein kinase phosphorylation (AMPK)	764:814	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	0	12	theme	intestinal	67:76	arg1	homeostasis					78:88	intestinal homeostasis	67:88	intestinal homeostasis	67:88	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	3	13	theme	inflammation	707:718	arg1	upregulation					680:691	upregulation	680:691	upregulation of intestinal inflammation	680:718	Esrra-deficient mice presented with increased susceptibility to dextran sodium sulfate (DSS)-induced colitis with upregulation of intestinal inflammation.					
33172329	4	14	theme	SQSTM1/p62	885:894	arg1	levels					823:828	lower levels	817:828	lower levels of TFEB (transcription factor EB)	817:862	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	14	theme	SQSTM1/p62	885:894	arg1	AMPK					810:813	AMPK	810:813	AMPK	810:813	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	14	theme	SQSTM1/p62	885:894	arg1	accumulation					869:880	accumulation	869:880	accumulation of SQSTM1/p62 (sequestosome 1)	869:911	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	14	theme	SQSTM1/p62	885:894	arg1	phosphorylation					793:807	AMP-activated protein kinase phosphorylation	764:807	AMP-activated protein kinase phosphorylation (AMPK)	764:814	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	7	15	theme	ESRRA	1303:1307	arg1	expression					1309:1318	decreased ESRRA expression	1293:1318	decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC	1293:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	5	16	theme	distinct	992:999	arg1	composition					1016:1026	distinct gut microbiota composition	992:1026	distinct gut microbiota composition	992:1026	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	7	17	from	UC	1431:1432	arg1	relevance					1409:1417	clinical relevance	1400:1417	clinical relevance of ESRRA in UC	1400:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	2	18	theme	flux	525:528	arg1	activation					500:509	activation	500:509	activation of autophagic flux	500:528	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	2	18	theme	flux	525:528	arg1	control					534:540	control	534:540	control of host gut microbiota	534:563	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	6	19	theme	fecal	1112:1116	arg1	transplantation					1129:1143	fecal microbiota transplantation	1112:1143	fecal microbiota transplantation	1112:1143	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	5	20	theme	gut	1001:1003	arg1	composition					1016:1026	distinct gut microbiota composition	992:1026	distinct gut microbiota composition	992:1026	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	7	21	from	ESRRA	1422:1426	arg1	UC					1431:1432	UC	1431:1432	UC	1431:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	1	22	theme	orphan	152:157	arg1	alpha					209:213	estrogen related receptor alpha	183:213	estrogen related receptor alpha	183:213	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	22	theme	orphan	152:157	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	22	theme	orphan	152:157	arg1	critical					219:226	critical	219:226	critical	219:226	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	2	23	theme	autophagic	514:523	arg1	flux					525:528	autophagic flux	514:528	autophagic flux	514:528	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	6	24	theme	DSS-induced	1194:1204	arg1	colitis					1206:1212	DSS-induced colitis	1194:1212	DSS-induced colitis severity	1194:1221	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	5	25	theme	microbiota	1005:1014	arg1	composition					1016:1026	distinct gut microbiota composition	992:1026	distinct gut microbiota composition	992:1026	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	4	26	theme	intestinal	944:953	arg1	tissues					955:961	intestinal tissues	944:961	intestinal tissues	944:961	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	1	27	theme	nuclear	159:165	arg1	alpha					209:213	estrogen related receptor alpha	183:213	estrogen related receptor alpha	183:213	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	27	theme	nuclear	159:165	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	27	theme	nuclear	159:165	arg1	critical					219:226	critical	219:226	critical	219:226	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	2	28	theme	homeostasis	440:450	arg1	regulator					416:424	a key regulator	410:424	a key regulator of intestinal homeostasis	410:450	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	2	28	theme	homeostasis	440:450	arg1	ESRRA					396:400	ESRRA	396:400	ESRRA	396:400	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	4	29	theme	AMP-activated	764:776	arg1	kinase					786:791	AMP-activated protein kinase	764:791	AMP-activated protein kinase phosphorylation (AMPK)	764:814	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	0	30	theme	gut	132:134	arg1	microbiota					136:145	gut microbiota	132:145	gut microbiota	132:145	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	1	31	theme	receptor	167:174	arg1	alpha					209:213	estrogen related receptor alpha	183:213	estrogen related receptor alpha	183:213	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	31	theme	receptor	167:174	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	31	theme	receptor	167:174	arg1	critical					219:226	critical	219:226	critical	219:226	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	2	32	theme	intestinal	429:438	arg1	homeostasis					440:450	intestinal homeostasis	429:450	intestinal homeostasis	429:450	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	1	33	theme	macroautophagy/autophagy	260:283	arg1	function					285:292	macroautophagy/autophagy function	260:292	macroautophagy/autophagy function	260:292	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	34	from	function	285:292	arg1	critical					219:226	critical	219:226	critical	219:226	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	34	from	function	285:292	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	6	35	theme	colitis	1206:1212	arg1	severity					1214:1221	DSS-induced colitis severity	1194:1221	DSS-induced colitis severity	1194:1221	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	0	36	theme	related	16:22	arg1	alpha					33:37	estrogen related receptor alpha	7:37	estrogen related receptor alpha	7:37	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	0	36	theme	related	16:22	arg1	ESRRA					0:4	ESRRA	0:4	ESRRA (estrogen related receptor alpha)	0:38	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	0	37	theme	autophagic	112:121	arg1	flux					123:126	autophagic flux	112:126	autophagic flux	112:126	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	4	38	theme	transcription	839:851	arg1	TFEB					833:836	TFEB	833:836	TFEB (transcription factor EB)	833:862	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	38	theme	transcription	839:851	arg1	factor					853:858	transcription factor EB	839:861	transcription factor EB	839:861	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	39	theme	kinase	786:791	arg1	AMPK					810:813	AMPK	810:813	AMPK	810:813	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	39	theme	kinase	786:791	arg1	phosphorylation					793:807	AMP-activated protein kinase phosphorylation	764:807	AMP-activated protein kinase phosphorylation (AMPK)	764:814	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	2	40	theme	key	412:414	arg1	regulator					416:424	a key regulator	410:424	a key regulator of intestinal homeostasis	410:450	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	2	40	theme	key	412:414	arg1	ESRRA					396:400	ESRRA	396:400	ESRRA	396:400	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	0	41	theme	estrogen	7:14	arg1	alpha					33:37	estrogen related receptor alpha	7:37	estrogen related receptor alpha	7:37	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	0	41	theme	estrogen	7:14	arg1	ESRRA					0:4	ESRRA	0:4	ESRRA (estrogen related receptor alpha)	0:38	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	2	42	theme	gut	550:552	arg1	microbiota					554:563	host gut microbiota	545:563	host gut microbiota	545:563	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	3	43	theme	-induced	658:665	arg1	colitis					667:673	dextran sodium sulfate (DSS)-induced colitis	630:673	dextran sodium sulfate (DSS)-induced colitis	630:673	Esrra-deficient mice presented with increased susceptibility to dextran sodium sulfate (DSS)-induced colitis with upregulation of intestinal inflammation.					
33172329	3	44	theme	Esrra-deficient	566:580	arg1	mice					582:585	Esrra-deficient mice	566:585	Esrra-deficient mice	566:585	Esrra-deficient mice presented with increased susceptibility to dextran sodium sulfate (DSS)-induced colitis with upregulation of intestinal inflammation.					
33172329	1	45	theme	estrogen	183:190	arg1	alpha					209:213	estrogen related receptor alpha	183:213	estrogen related receptor alpha	183:213	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	45	theme	estrogen	183:190	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	2	46	theme	colonic	471:477	arg1	inflammation					479:490	colonic inflammation	471:490	colonic inflammation	471:490	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	2	47	theme	host	545:548	arg1	microbiota					554:563	host gut microbiota	545:563	host gut microbiota	545:563	Herein we identified that ESRRA acts as a key regulator of intestinal homeostasis by amelioration of colonic inflammation through activation of autophagic flux and control of host gut microbiota.					
33172329	7	48	theme	ulcerative	1251:1260	arg1	colitis					1262:1268	ulcerative colitis	1251:1268	ulcerative colitis (UC)	1251:1273	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	7	48	theme	ulcerative	1251:1260	arg1	UC					1271:1272	UC	1271:1272	UC	1271:1272	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	5	49	theme	microbial	1053:1061	arg1	diversity					1063:1071	significantly higher microbial diversity	1032:1071	significantly higher microbial diversity	1032:1071	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	1	50	theme	related	192:198	arg1	alpha					209:213	estrogen related receptor alpha	183:213	estrogen related receptor alpha	183:213	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	50	theme	related	192:198	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	0	51	theme	receptor	24:31	arg1	alpha					33:37	estrogen related receptor alpha	7:37	estrogen related receptor alpha	7:37	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	0	51	theme	receptor	24:31	arg1	ESRRA					0:4	ESRRA	0:4	ESRRA (estrogen related receptor alpha)	0:38	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	7	52	with	patients	1237:1244	arg1	colitis					1262:1268	ulcerative colitis	1251:1268	ulcerative colitis (UC)	1251:1273	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	7	52	with	patients	1237:1244	arg1	UC					1271:1272	UC	1271:1272	UC	1271:1272	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	0	53	theme	flux	123:126	arg1	activation					98:107	activation	98:107	activation of autophagic flux via gut microbiota	98:145	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	4	54	from	mitochondria	928:939	arg1	tissues					955:961	intestinal tissues	944:961	intestinal tissues	944:961	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	55	theme	TFEB	833:836	arg1	levels					823:828	lower levels	817:828	lower levels of TFEB (transcription factor EB)	817:862	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	55	theme	TFEB	833:836	arg1	AMPK					810:813	AMPK	810:813	AMPK	810:813	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	55	theme	TFEB	833:836	arg1	accumulation					869:880	accumulation	869:880	accumulation of SQSTM1/p62 (sequestosome 1)	869:911	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	4	55	theme	TFEB	833:836	arg1	phosphorylation					793:807	AMP-activated protein kinase phosphorylation	764:807	AMP-activated protein kinase phosphorylation (AMPK)	764:814	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	1	56	theme	receptor	200:207	arg1	alpha					209:213	estrogen related receptor alpha	183:213	estrogen related receptor alpha	183:213	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	56	theme	receptor	200:207	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	6	57	from	mice	1153:1156	arg1	Cohousing					1099:1107	Cohousing	1099:1107	Cohousing	1099:1107	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	6	57	from	mice	1153:1156	arg1	transplantation					1129:1143	fecal microbiota transplantation	1112:1143	fecal microbiota transplantation	1112:1143	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	4	58	theme	esrra-null	734:743	arg1	mice					745:748	esrra-null mice	734:748	esrra-null mice	734:748	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	1	59	from	biogenesis	245:254	arg1	critical					219:226	critical	219:226	critical	219:226	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	59	from	biogenesis	245:254	arg1	ESRRA					176:180	The orphan nuclear receptor ESRRA	148:180	The orphan nuclear receptor ESRRA (estrogen related receptor alpha)	148:214	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	60	from	critical	219:226	arg1	biogenesis					245:254	mitochondrial biogenesis	231:254	mitochondrial biogenesis	231:254	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	1	60	from	critical	219:226	arg1	function					285:292	macroautophagy/autophagy function	260:292	macroautophagy/autophagy function	260:292	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	3	61	theme	increased	602:610	arg1	susceptibility					612:625	increased susceptibility	602:625	increased susceptibility to dextran sodium sulfate (DSS)-induced colitis	602:673	Esrra-deficient mice presented with increased susceptibility to dextran sodium sulfate (DSS)-induced colitis with upregulation of intestinal inflammation.					
33172329	6	62	theme	Esrra-deficient	1161:1175	arg1	mice					1177:1180	Esrra-deficient mice	1161:1180	Esrra-deficient mice	1161:1180	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	7	63	theme	ESRRA	1422:1426	arg1	relevance					1409:1417	clinical relevance	1400:1417	clinical relevance of ESRRA in UC	1400:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	3	64	theme	intestinal	696:705	arg1	inflammation					707:718	intestinal inflammation	696:718	intestinal inflammation	696:718	Esrra-deficient mice presented with increased susceptibility to dextran sodium sulfate (DSS)-induced colitis with upregulation of intestinal inflammation.					
33172329	4	65	theme	lower	817:821	arg1	levels					823:828	lower levels	817:828	lower levels of TFEB (transcription factor EB)	817:862	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	7	66	theme	intestinal	1323:1332	arg1	tissues					1342:1348	intestinal mucosal tissues	1323:1348	intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC	1323:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	5	67	theme	Esrra-deficient	964:978	arg1	mice					980:983	Esrra-deficient mice	964:983	Esrra-deficient mice	964:983	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	7	68	contain	had	1275:1277	arg2	expression					1309:1318	decreased ESRRA expression	1293:1318	decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC	1293:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	7	68	contain	had	1275:1277	arg1	patients					1237:1244	patients	1237:1244	patients with ulcerative colitis (UC)	1237:1273	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	7	69	theme	mucosal	1334:1340	arg1	tissues					1342:1348	intestinal mucosal tissues	1323:1348	intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC	1323:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	4	70	theme	defective	918:926	arg1	mitochondria					928:939	defective mitochondria	918:939	defective mitochondria in intestinal tissues	918:961	In addition, esrra-null mice had depressed AMP-activated protein kinase phosphorylation (AMPK), lower levels of TFEB (transcription factor EB), and accumulation of SQSTM1/p62 (sequestosome 1) with defective mitochondria in intestinal tissues.					
33172329	0	71	theme	critical	45:52	arg1	regulator					54:62	a critical regulator	43:62	a critical regulator of intestinal homeostasis	43:88	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	0	71	theme	critical	45:52	arg1	ESRRA					0:4	ESRRA	0:4	ESRRA (estrogen related receptor alpha)	0:38	ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota.					
33172329	7	72	theme	disease	1371:1377	arg1	activity					1379:1386	disease activity	1371:1386	disease activity	1371:1386	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
33172329	6	73	theme	WT	1150:1151	arg1	mice					1153:1156	WT mice	1150:1156	WT mice	1150:1156	Cohousing or fecal microbiota transplantation from WT mice to Esrra-deficient mice ameliorated DSS-induced colitis severity.					
33172329	5	74	dep	wild-type	1078:1086	arg1	WT					1089:1090	WT	1089:1090	WT	1089:1090	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	1	75	theme	ESRRA	317:321	arg1	roles					308:312	the roles	304:312	the roles of ESRRA in intestinal function	304:344	The orphan nuclear receptor ESRRA (estrogen related receptor alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of ESRRA in intestinal function remain uncharacterized.					
33172329	5	76	theme	higher	1046:1051	arg1	diversity					1063:1071	significantly higher microbial diversity	1032:1071	significantly higher microbial diversity	1032:1071	Esrra-deficient mice showed distinct gut microbiota composition and significantly higher microbial diversity than wild-type (WT) mice.					
33172329	7	77	theme	decreased	1293:1301	arg1	expression					1309:1318	decreased ESRRA expression	1293:1318	decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC	1293:1432	Importantly, patients with ulcerative colitis (UC) had significantly decreased ESRRA expression in intestinal mucosal tissues that correlated with disease activity, suggesting clinical relevance of ESRRA in UC.					
32245231	2	0	theme	local	463:467	arg1	dynamics					469:476	local dynamics	463:476	local dynamics	463:476	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	0	1	from	Study	14:18	arg1	Trehalose					23:31	Trehalose	23:31	Trehalose	23:31	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.					
32245231	0	1	from	Study	14:18	arg1	Mixtures					41:48	Water Mixtures	35:48	Water Mixtures	35:48	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.					
32245231	2	2	dep	interactions	436:447	arg1	energies					449:456	energies	449:456	energies	449:456	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	0	3	theme	Preservation	58:69	arg1	Purposes					71:78	Dry Preservation Purposes	54:78	Dry Preservation Purposes	54:78	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.					
32245231	3	4	theme	trehalose	678:686	arg1	effects					667:673	the effects	663:673	the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent	663:793	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	2	5	theme	preservation	281:292	arg1	purposes					294:301	dry preservation purposes	277:301	dry preservation purposes	277:301	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	6	theme	relationships	489:501	arg1	terms					402:406	terms	402:406	terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding	402:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	7	theme	dry	277:279	arg1	purposes					294:301	dry preservation purposes	277:301	dry preservation purposes	277:301	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	8	with	dynamics	469:476	arg1	bonding					517:523	hydrogen bonding	508:523	hydrogen bonding	508:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	1	9	dep	trehalose	99:107	arg1	mixtures					117:124	mixtures	117:124	mixtures	117:124	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	2	10	theme	considered	378:387	arg1	mixtures					389:396	the considered mixtures	374:396	the considered mixtures	374:396	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	0	11	theme	Theoretical	2:12	arg1	Study					14:18	A Theoretical Study	0:18	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.	0:79	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.					
32245231	3	12	theme	providing	709:717	arg1	results					719:725	providing results	709:725	providing results	709:725	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	3	13	with	evolution	609:617	arg1	composition					632:642	mixture composition	624:642	mixture composition	624:642	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	3	14	with	bonding	593:599	arg1	composition					632:642	mixture composition	624:642	mixture composition	624:642	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	1	15	theme	mixture	155:161	arg1	composition					163:173	mixture composition	155:173	mixture composition	155:173	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	1	16	theme	composition	163:173	arg1	function					143:150	a function	141:150	a function of mixture composition and temperature	141:189	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	1	16	theme	composition	163:173	arg1	properties					85:94	The properties	81:94	The properties of trehalose + water mixtures	81:124	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	2	17	theme	aggregation	411:421	arg1	terms					402:406	terms	402:406	terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding	402:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	18	with	relationships	489:501	arg1	bonding					517:523	hydrogen bonding	508:523	hydrogen bonding	508:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	3	19	theme	reported	530:537	arg1	results					539:545	The reported results	526:545	The reported results	526:545	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	1	20	theme	temperature	179:189	arg1	function					143:150	a function	141:150	a function of mixture composition and temperature	141:189	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	1	20	theme	temperature	179:189	arg1	properties					85:94	The properties	81:94	The properties of trehalose + water mixtures	81:124	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	3	21	from	effects	667:673	arg1	water					691:695	water	691:695	water structuring providing results to justify the mechanisms of trehalose acting as preservation agent	691:793	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	2	22	theme	hydrogen	508:515	arg1	bonding					517:523	hydrogen bonding	508:523	hydrogen bonding	508:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	3	23	theme	hydrogen	584:591	arg1	bonding					593:599	hydrogen bonding	584:599	hydrogen bonding	584:599	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	2	24	with	aggregation	411:421	arg1	bonding					517:523	hydrogen bonding	508:523	hydrogen bonding	508:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	1	25	theme	molecular	197:205	arg1	simulations					216:226	molecular dynamics simulations	197:226	molecular dynamics simulations	197:226	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	2	26	theme	interactions	436:447	arg1	terms					402:406	terms	402:406	terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding	402:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	3	27	theme	preservation	776:787	arg1	agent					789:793	preservation agent	776:793	preservation agent	776:793	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	3	28	theme	mixture	624:630	arg1	composition					632:642	mixture composition	624:642	mixture composition	624:642	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	3	29	theme	detailed	555:562	arg1	characterization					564:579	a detailed characterization	553:579	a detailed characterization of hydrogen bonding and its evolution with mixture composition	553:642	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	1	30	theme	trehalose	99:107	arg1	function					143:150	a function	141:150	a function of mixture composition and temperature	141:189	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	1	30	theme	trehalose	99:107	arg1	properties					85:94	The properties	81:94	The properties of trehalose + water mixtures	81:124	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	0	31	theme	Water	35:39	arg1	Mixtures					41:48	Water Mixtures	35:48	Water Mixtures	35:48	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.					
32245231	3	32	theme	trehalose	756:764	arg1	mechanisms					742:751	the mechanisms	738:751	the mechanisms of trehalose acting as preservation agent	738:793	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	2	33	with	clustering	424:433	arg1	bonding					517:523	hydrogen bonding	508:523	hydrogen bonding	508:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	34	theme	work	326:329	arg1	objective					308:316	the objective	304:316	the objective of this work	304:329	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	1	35	theme	water	111:115	arg1	function					143:150	a function	141:150	a function of mixture composition and temperature	141:189	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	1	35	theme	water	111:115	arg1	properties					85:94	The properties	81:94	The properties of trehalose + water mixtures	81:124	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	1	36	theme	dynamics	207:214	arg1	simulations					216:226	molecular dynamics simulations	197:226	molecular dynamics simulations	197:226	The properties of trehalose + water mixtures are studied as a function of mixture composition and temperature using molecular dynamics simulations.					
32245231	3	37	theme	evolution	609:617	arg1	characterization					564:579	a detailed characterization	553:579	a detailed characterization of hydrogen bonding and its evolution with mixture composition	553:642	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	2	38	with	interactions	436:447	arg1	bonding					517:523	hydrogen bonding	508:523	hydrogen bonding	508:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	0	39	theme	Dry	54:56	arg1	Purposes					71:78	Dry Preservation Purposes	54:78	Dry Preservation Purposes	54:78	A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.					
32245231	2	40	theme	mixtures	389:396	arg1	properties					360:369	the nanoscopic properties	345:369	the nanoscopic properties of the considered mixtures	345:396	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	3	41	theme	bonding	593:599	arg1	characterization					564:579	a detailed characterization	553:579	a detailed characterization of hydrogen bonding and its evolution with mixture composition	553:642	The reported results allow a detailed characterization of hydrogen bonding and its evolution with mixture composition and thus inferring the effects of trehalose on water structuring providing results to justify the mechanisms of trehalose acting as preservation agent.					
32245231	2	42	theme	nanoscopic	349:358	arg1	properties					360:369	the nanoscopic properties	345:369	the nanoscopic properties of the considered mixtures	345:396	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	43	theme	trehalose	232:240	arg1	disaccharide					242:253	trehalose disaccharide	232:253	trehalose disaccharide	232:253	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32245231	2	44	theme	clustering	424:433	arg1	terms					402:406	terms	402:406	terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding	402:523	As trehalose disaccharide has been proposed for dry preservation purposes, the objective of this work is to analyse the nanoscopic properties of the considered mixtures, in terms of aggregation, clustering, interactions energies, and local dynamics, and their relationships with hydrogen bonding.					
32466125	11	0	theme	microbial	1563:1571	arg1	profiles					1573:1580	gut microbial profiles	1559:1580	gut microbial profiles	1559:1580	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	2	1	theme	individual	366:375	arg1	HMOs					377:380	individual HMOs	366:380	individual HMOs	366:380	Our objective was to determine whether supplementing individual HMOs to a weanling diet alters growth and gut health in rats.					
32466125	10	2	theme	complex	1480:1486	arg1	profile					1515:1521	a complex sex-dependent risk/benefit profile	1478:1521	a complex sex-dependent risk/benefit profile	1478:1521	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	4	3	from	composition	654:664	arg1	tract					799:803	the gastrointestinal tract	778:803	the gastrointestinal tract	778:803	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	11	4	with	profiles	1573:1580	arg1	HMOs					1613:1616	other HMOs	1607:1616	other HMOs	1607:1616	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	8	5	theme	HMO-fortified	1148:1160	arg1	groups					1162:1167	All female HMO-fortified groups	1137:1167	All female HMO-fortified groups	1137:1167	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	10	6	theme	risk/benefit	1502:1513	arg1	profile					1515:1521	a complex sex-dependent risk/benefit profile	1478:1521	a complex sex-dependent risk/benefit profile	1478:1521	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	0	7	theme	Young	138:142	arg1	Rats					159:162	Young Sprague Dawley Rats	138:162	Young Sprague Dawley Rats	138:162	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	3	8	theme	2'-O-fucosyllactose	514:532	arg1	2'FL					535:538	2'-O-fucosyllactose (2'FL)-	514:540	2'-O-fucosyllactose (2'FL)-	514:540	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	1	9	theme	maternal	210:217	arg1	oligosaccharides					176:191	Human milk oligosaccharides	165:191	Human milk oligosaccharides (HMOs)	165:198	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	9	theme	maternal	210:217	arg1	constituents					224:235	chief maternal milk constituents	204:235	chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut	204:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	0	10	from	Composition	93:103	arg1	Tract					129:133	the Gastrointestinal Tract	108:133	the Gastrointestinal Tract of Young Sprague Dawley Rats	108:162	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	0	11	theme	Dawley	152:157	arg1	Rats					159:162	Young Sprague Dawley Rats	138:162	Young Sprague Dawley Rats	138:162	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	8	12	theme	intestinal	1205:1214	arg1	permeability					1216:1227	intestinal permeability	1205:1227	intestinal permeability	1205:1227	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	3	13	theme	-fortified	568:577	arg1	diets					579:583	-fortified diets	568:583	-fortified diets	568:583	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	10	14	with	Supplementation	1409:1423	arg1	fraction					1432:1439	a fraction	1430:1439	a fraction of the HMOs found in breast milk	1430:1472	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	4	15	theme	fecal	709:713	arg1	composition					726:736	fecal microbiota composition	709:736	fecal microbiota composition	709:736	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	8	16	from	reductions	1191:1200	arg1	permeability					1216:1227	intestinal permeability	1205:1227	intestinal permeability	1205:1227	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	4	17	theme	intestinal	667:676	arg1	permeability					678:689	intestinal permeability	667:689	intestinal permeability	667:689	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	0	18	theme	Oligosaccharide	11:25	arg1	Supplementation					27:41	Human Milk Oligosaccharide Supplementation	0:41	Human Milk Oligosaccharide Supplementation	0:41	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	4	19	from	expression	764:773	arg1	tract					799:803	the gastrointestinal tract	778:803	the gastrointestinal tract	778:803	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	1	20	theme	Human	165:169	arg1	constituents					224:235	chief maternal milk constituents	204:235	chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut	204:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	20	theme	Human	165:169	arg1	HMOs					194:197	HMOs	194:197	HMOs	194:197	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	20	theme	Human	165:169	arg1	oligosaccharides					176:191	Human milk oligosaccharides	165:191	Human milk oligosaccharides (HMOs)	165:198	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	4	21	theme	RNA	753:755	arg1	expression					764:773	messenger RNA (mRNA) expression	743:773	messenger RNA (mRNA) expression	743:773	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	3	22	theme	Healthy	439:445	arg1	pups					481:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	0	23	theme	Intestinal	51:60	arg1	Function					70:77	Intestinal Barrier Function	51:77	Intestinal Barrier Function	51:77	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	3	24	dep	control	505:511	arg1	diets					579:583	-fortified diets	568:583	-fortified diets	568:583	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	0	25	theme	Microbial	83:91	arg1	Composition					93:103	Microbial Composition	83:103	Microbial Composition	83:103	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	0	26	theme	Human	0:4	arg1	Supplementation					27:41	Human Milk Oligosaccharide Supplementation	0:41	Human Milk Oligosaccharide Supplementation	0:41	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	4	27	from	composition	726:736	arg1	tract					799:803	the gastrointestinal tract	778:803	the gastrointestinal tract	778:803	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	6	28	theme	body	992:995	arg1	composition					997:1007	body composition	992:1007	body composition	992:1007	No differences in body composition were detected between groups.					
32466125	10	29	located	found	1453:1457	arg2	HMOs					1448:1451	the HMOs	1444:1451	the HMOs found in breast milk	1444:1472	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	10	29	located	found	1453:1457	arg1	milk					1469:1472	breast milk	1462:1472	breast milk	1462:1472	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	3	30	theme	rat	477:479	arg1	pups					481:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	7	31	theme	2	1087:1087	arg1	SL					1096:1097	2'FL + 3'SL	1087:1097	2'FL + 3'SL	1087:1097	In females, HMOs did not affect body weight but 2'FL + 3'SL significantly increased cecum weight.					
32466125	1	32	theme	infant	301:306	arg1	gut					308:310	the infant gut	297:310	the infant gut	297:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	5	33	theme	=	908:908	arg1	p					906:906	p = 0.01	906:913	p = 0.01	906:913	Males fed a control diet were 10% heavier and displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11.					
32466125	5	33	theme	=	908:908	arg1	interleukin					885:895	interleukin	885:895	interleukin (IL-18) (p = 0.01) in serum	885:923	Males fed a control diet were 10% heavier and displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11.					
32466125	5	34	theme	control	832:838	arg1	diet					840:843	a control diet	830:843	a control diet	830:843	Males fed a control diet were 10% heavier and displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11.					
32466125	10	35	theme	HMOs	1448:1451	arg1	fraction					1432:1439	a fraction	1430:1439	a fraction of the HMOs found in breast milk	1430:1472	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	11	36	theme	Further	1524:1530	arg1	investigation					1542:1554	Further long-term investigation	1524:1554	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development	1524:1641	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	0	37	theme	Barrier	62:68	arg1	Function					70:77	Intestinal Barrier Function	51:77	Intestinal Barrier Function	51:77	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	2	38	from	growth	408:413	arg1	rats					433:436	rats	433:436	rats	433:436	Our objective was to determine whether supplementing individual HMOs to a weanling diet alters growth and gut health in rats.					
32466125	11	39	theme	early	1625:1629	arg1	development					1631:1641	early development	1625:1641	early development	1625:1641	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	9	40	theme	gut	1294:1296	arg1	composition					1309:1319	gut microbiota composition	1294:1319	gut microbiota composition	1294:1319	All HMO-fortified diets altered gut microbiota composition and mRNA expression in the gastrointestinal tract, albeit differently according to sex.					
32466125	2	41	from	gut	419:421	arg1	rats					433:436	rats	433:436	rats	433:436	Our objective was to determine whether supplementing individual HMOs to a weanling diet alters growth and gut health in rats.					
32466125	2	42	dep	growth	408:413	arg1	health					423:428	health	423:428	health	423:428	Our objective was to determine whether supplementing individual HMOs to a weanling diet alters growth and gut health in rats.					
32466125	11	43	theme	gut	1559:1561	arg1	profiles					1573:1580	gut microbial profiles	1559:1580	gut microbial profiles	1559:1580	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	5	44	theme	HMO-fortified	941:953	arg1	groups					955:960	all HMO-fortified groups	937:960	all HMO-fortified groups	937:960	Males fed a control diet were 10% heavier and displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11.					
32466125	11	45	theme	supplementation	1586:1600	arg1	investigation					1542:1554	Further long-term investigation	1524:1554	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development	1524:1641	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	4	46	from	permeability	678:689	arg1	tract					799:803	the gastrointestinal tract	778:803	the gastrointestinal tract	778:803	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	9	47	theme	mRNA	1325:1328	arg1	expression					1330:1339	mRNA expression	1325:1339	mRNA expression	1325:1339	All HMO-fortified diets altered gut microbiota composition and mRNA expression in the gastrointestinal tract, albeit differently according to sex.					
32466125	10	48	theme	sex-dependent	1488:1500	arg1	profile					1515:1521	a complex sex-dependent risk/benefit profile	1478:1521	a complex sex-dependent risk/benefit profile	1478:1521	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	11	49	theme	profiles	1573:1580	arg1	investigation					1542:1554	Further long-term investigation	1524:1554	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development	1524:1641	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	11	50	with	supplementation	1586:1600	arg1	HMOs					1613:1616	other HMOs	1607:1616	other HMOs	1607:1616	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	9	51	theme	gastrointestinal	1348:1363	arg1	tract					1365:1369	the gastrointestinal tract	1344:1369	the gastrointestinal tract	1344:1369	All HMO-fortified diets altered gut microbiota composition and mRNA expression in the gastrointestinal tract, albeit differently according to sex.					
32466125	11	52	theme	other	1607:1611	arg1	HMOs					1613:1616	other HMOs	1607:1616	other HMOs	1607:1616	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	8	53	theme	female	1141:1146	arg1	groups					1162:1167	All female HMO-fortified groups	1137:1167	All female HMO-fortified groups	1137:1167	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	6	54	from	differences	977:987	arg1	composition					997:1007	body composition	992:1007	body composition	992:1007	No differences in body composition were detected between groups.					
32466125	1	55	theme	chief	204:208	arg1	oligosaccharides					176:191	Human milk oligosaccharides	165:191	Human milk oligosaccharides (HMOs)	165:198	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	55	theme	chief	204:208	arg1	constituents					224:235	chief maternal milk constituents	204:235	chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut	204:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	0	56	theme	Rats	159:162	arg1	Tract					129:133	the Gastrointestinal Tract	108:133	the Gastrointestinal Tract of Young Sprague Dawley Rats	108:162	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	4	57	theme	Body	649:652	arg1	composition					654:664	Body composition	649:664	Body composition	649:664	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	1	58	theme	milk	219:222	arg1	oligosaccharides					176:191	Human milk oligosaccharides	165:191	Human milk oligosaccharides (HMOs)	165:198	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	58	theme	milk	219:222	arg1	constituents					224:235	chief maternal milk constituents	204:235	chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut	204:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	8	59	theme	significant	1179:1189	arg1	reductions					1191:1200	significant reductions	1179:1200	significant reductions in intestinal permeability	1179:1227	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	4	60	theme	serum	692:696	arg1	cytokines					698:706	serum cytokines	692:706	serum cytokines	692:706	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	7	61	theme	cecum	1123:1127	arg1	weight					1129:1134	cecum weight	1123:1134	cecum weight	1123:1134	In females, HMOs did not affect body weight but 2'FL + 3'SL significantly increased cecum weight.					
32466125	1	62	theme	milk	171:174	arg1	constituents					224:235	chief maternal milk constituents	204:235	chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut	204:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	62	theme	milk	171:174	arg1	HMOs					194:197	HMOs	194:197	HMOs	194:197	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	1	62	theme	milk	171:174	arg1	oligosaccharides					176:191	Human milk oligosaccharides	165:191	Human milk oligosaccharides (HMOs)	165:198	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	7	63	dep	2	1087:1087	arg1	3					1094:1094	3	1094:1094	3	1094:1094	In females, HMOs did not affect body weight but 2'FL + 3'SL significantly increased cecum weight.					
32466125	7	63	dep	2	1087:1087	arg1	FL					1089:1090	FL	1089:1090	FL	1089:1090	In females, HMOs did not affect body weight but 2'FL + 3'SL significantly increased cecum weight.					
32466125	0	64	theme	Gastrointestinal	112:127	arg1	Tract					129:133	the Gastrointestinal Tract	108:133	the Gastrointestinal Tract of Young Sprague Dawley Rats	108:162	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	0	65	from	Function	70:77	arg1	Tract					129:133	the Gastrointestinal Tract	108:133	the Gastrointestinal Tract of Young Sprague Dawley Rats	108:162	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	4	66	theme	messenger	743:751	arg1	RNA					753:755	messenger RNA	743:755	messenger RNA (mRNA) expression	743:773	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	4	66	theme	messenger	743:751	arg1	mRNA					758:761	mRNA	758:761	mRNA	758:761	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	8	67	theme	=	1253:1253	arg1	controls					1241:1248	controls	1241:1248	controls (p = 0.02)	1241:1259	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	8	67	theme	=	1253:1253	arg1	p					1251:1251	p = 0.02	1251:1258	p = 0.02	1251:1258	All female HMO-fortified groups displayed significant reductions in intestinal permeability compared to controls (p = 0.02).					
32466125	3	68	theme	three-week-old	447:460	arg1	pups					481:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	0	69	theme	Milk	6:9	arg1	Supplementation					27:41	Human Milk Oligosaccharide Supplementation	0:41	Human Milk Oligosaccharide Supplementation	0:41	Human Milk Oligosaccharide Supplementation Affects Intestinal Barrier Function and Microbial Composition in the Gastrointestinal Tract of Young Sprague Dawley Rats.					
32466125	10	70	contain	has	1474:1476	arg1	Supplementation					1409:1423	Supplementation	1409:1423	Supplementation with a fraction of the HMOs found in breast milk	1409:1472	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	10	70	contain	has	1474:1476	arg2	profile					1515:1521	a complex sex-dependent risk/benefit profile	1478:1521	a complex sex-dependent risk/benefit profile	1478:1521	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	4	71	theme	microbiota	715:724	arg1	composition					726:736	fecal microbiota composition	709:736	fecal microbiota composition	709:736	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	2	72	theme	weanling	387:394	arg1	diet					396:399	a weanling diet	385:399	a weanling diet	385:399	Our objective was to determine whether supplementing individual HMOs to a weanling diet alters growth and gut health in rats.					
32466125	5	73	from	interleukin	885:895	arg1	serum					919:923	serum	919:923	serum	919:923	Males fed a control diet were 10% heavier and displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11.					
32466125	7	74	theme	body	1071:1074	arg1	weight					1076:1081	body weight	1071:1081	body weight	1071:1081	In females, HMOs did not affect body weight but 2'FL + 3'SL significantly increased cecum weight.					
32466125	1	75	theme	gut	308:310	arg1	maturation					283:292	maturation	283:292	maturation of the infant gut	283:310	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
32466125	3	76	theme	physiological	612:624	arg1	doses					626:630	physiological doses	612:630	physiological doses for eight weeks	612:646	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	4	77	theme	gastrointestinal	782:797	arg1	tract					799:803	the gastrointestinal tract	778:803	the gastrointestinal tract	778:803	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	3	78	theme	Dawley	470:475	arg1	pups					481:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups	439:484	Healthy three-week-old Sprague Dawley rat pups were randomized to control, 2'-O-fucosyllactose (2'FL)- and 3'sialyllactose (3'SL)-fortified diets alone or in combination at physiological doses for eight weeks.					
32466125	9	79	theme	HMO-fortified	1266:1278	arg1	diets					1280:1284	All HMO-fortified diets	1262:1284	All HMO-fortified diets	1262:1284	All HMO-fortified diets altered gut microbiota composition and mRNA expression in the gastrointestinal tract, albeit differently according to sex.					
32466125	5	80	dep	displayed	866:874	arg1	elevated					876:883	elevated	876:883	displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11	866:971	Males fed a control diet were 10% heavier and displayed elevated interleukin (IL-18) (p = 0.01) in serum compared to all HMO-fortified groups at week 11.					
32466125	4	81	from	cytokines	698:706	arg1	tract					799:803	the gastrointestinal tract	778:803	the gastrointestinal tract	778:803	Body composition, intestinal permeability, serum cytokines, fecal microbiota composition, and messenger RNA (mRNA) expression in the gastrointestinal tract were assessed.					
32466125	9	82	theme	microbiota	1298:1307	arg1	composition					1309:1319	gut microbiota composition	1294:1319	gut microbiota composition	1294:1319	All HMO-fortified diets altered gut microbiota composition and mRNA expression in the gastrointestinal tract, albeit differently according to sex.					
32466125	10	83	theme	breast	1462:1467	arg1	milk					1469:1472	breast milk	1462:1472	breast milk	1462:1472	Supplementation with a fraction of the HMOs found in breast milk has a complex sex-dependent risk/benefit profile.					
32466125	11	84	theme	long-term	1532:1540	arg1	investigation					1542:1554	Further long-term investigation	1524:1554	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development	1524:1641	Further long-term investigation of gut microbial profiles and supplementation with other HMOs during early development is warranted.					
32466125	1	85	theme	intestinal	251:260	arg1	microbiota					262:271	the intestinal microbiota	247:271	the intestinal microbiota	247:271	Human milk oligosaccharides (HMOs) are chief maternal milk constituents that feed the intestinal microbiota and drive maturation of the infant gut.					
34003829	3	0	theme	whole-body	706:715	arg1	composition					717:727	whole-body composition	706:727	whole-body composition of Nile tilapia	706:743	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	6	1	theme	selenomethionine	1321:1336	arg1	Se-Nano					1436:1442	Se-Nano	1436:1442	Se-Nano	1436:1442	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	1	theme	selenomethionine	1321:1336	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	1	theme	selenomethionine	1321:1336	arg1	Se-Nano					1448:1454	Se-Nano	1448:1454	Se-Nano	1448:1454	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	2	2	theme	First	342:346	arg1	nanoparticles					364:376	First, chitosan-based nanoparticles	342:376	First, chitosan-based nanoparticles containing selenium (Se)	342:401	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	11	3	theme	Nile	2147:2150	arg1	tilapia					2152:2158	Nile tilapia	2147:2158	Nile tilapia	2147:2158	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	8	4	theme	feed	1721:1724	arg1	efficiency					1726:1735	feed efficiency	1721:1735	feed efficiency	1721:1735	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	11	5	theme	growth	2184:2189	arg1	performance					2191:2201	growth performance	2184:2201	growth performance	2184:2201	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	6	6	theme	60-day	1206:1211	arg1	trial					1221:1225	A 60-day feeding trial	1204:1225	A 60-day feeding trial	1204:1225	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	3	7	theme	tilapia	737:743	arg1	peroxidase					622:631	glutathione peroxidase	610:631	glutathione peroxidase	610:631	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	3	7	theme	tilapia	737:743	arg1	activities					649:658	antioxidant activities	637:658	antioxidant activities	637:658	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	3	7	theme	tilapia	737:743	arg1	composition					717:727	whole-body composition	706:727	whole-body composition of Nile tilapia	706:743	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	3	7	theme	tilapia	737:743	arg1	growth					694:699	growth	694:699	growth	694:699	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	6	8	theme	Se	1299:1300	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	8	theme	Se	1299:1300	arg1	sources					1302:1308	supplementing different dietary Se sources	1267:1308	supplementing different dietary Se sources	1267:1308	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	11	9	theme	Se-loaded	2043:2051	arg1	nanoparticles					2053:2065	Se-loaded nanoparticles	2043:2065	Se-loaded nanoparticles	2043:2065	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	4	10	theme	high	911:914	arg1	efficiency					927:936	high entrapment efficiency	911:936	high entrapment efficiency (87%)	911:942	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	4	10	theme	high	911:914	arg1	%					941:941	87%	939:941	87%	939:941	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	6	11	theme	different	1281:1289	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	11	theme	different	1281:1289	arg1	sources					1302:1308	supplementing different dietary Se sources	1267:1308	supplementing different dietary Se sources	1267:1308	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	8	12	theme	tilapia	1745:1751	arg1	fingerlings					1753:1763	Nile tilapia fingerlings	1740:1763	Nile tilapia fingerlings	1740:1763	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	10	13	theme	antioxidant	1996:2006	arg1	activity					2008:2015	antioxidant activity	1996:2015	antioxidant activity in Nile tilapia	1996:2031	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	6	14	theme	selenite	1366:1373	arg1	Se-Nano					1436:1442	Se-Nano	1436:1442	Se-Nano	1436:1442	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	14	theme	selenite	1366:1373	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	14	theme	selenite	1366:1373	arg1	Se-Nano					1448:1454	Se-Nano	1448:1454	Se-Nano	1448:1454	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	2	15	contain	containing	378:387	arg2	Se					399:400	Se	399:400	Se	399:400	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	2	15	contain	containing	378:387	arg2	selenium					389:396	selenium	389:396	selenium (Se)	389:401	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	2	15	contain	containing	378:387	arg1	nanoparticles					364:376	First, chitosan-based nanoparticles	342:376	First, chitosan-based nanoparticles containing selenium (Se)	342:401	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	11	16	theme	great	2079:2083	arg1	potential					2085:2093	a great potential	2077:2093	a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species	2077:2278	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	6	17	theme	supplementing	1267:1279	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	17	theme	supplementing	1267:1279	arg1	sources					1302:1308	supplementing different dietary Se sources	1267:1308	supplementing different dietary Se sources	1267:1308	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	9	18	theme	lipid	1832:1836	arg1	content					1838:1844	whole-body protein and lipid content	1809:1844	content	1838:1844	However, dietary treatments did not affect whole-body protein and lipid content.					
34003829	1	19	from	effects	189:195	arg1	tilapia					276:282	Nile tilapia	271:282	Nile tilapia (Oreochromis niloticus)	271:306	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	1	19	from	effects	189:195	arg1	niloticus					297:305	Oreochromis niloticus	285:305	Oreochromis niloticus	285:305	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	10	20	theme	Nile	2020:2023	arg1	tilapia					2025:2031	Nile tilapia	2020:2031	Nile tilapia	2020:2031	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	11	21	dep	performance	2191:2201	arg1	the					2180:2182	the	2180:2182	the	2180:2182	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	3	22	theme	Se-loaded	784:792	arg1	nanoparticles					803:815	Se-loaded chitosan nanoparticles	784:815	Se-loaded chitosan nanoparticles	784:815	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	0	23	theme	Orechromis	132:141	arg1	tilapia					123:129	Nile tilapia	118:129	Nile tilapia (Orechromis niloticus)	118:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	23	theme	Orechromis	132:141	arg1	niloticus					143:151	Orechromis niloticus	132:151	Orechromis niloticus	132:151	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	3	24	theme	glutathione	610:620	arg1	peroxidase					622:631	glutathione peroxidase	610:631	glutathione peroxidase	610:631	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	4	25	theme	spherical	945:953	arg1	shape					955:959	spherical shape	945:959	spherical shape	945:959	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	3	26	theme	other	835:839	arg1	supplements					858:868	other selenium dietary supplements	835:868	other selenium dietary supplements	835:868	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	8	27	with	supplementations	1592:1607	arg1	sources					1627:1633	different Se sources	1614:1633	different Se sources (free and encapsulated selenium)	1614:1666	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	0	28	from	antioxidant	84:94	arg1	tilapia					123:129	Nile tilapia	118:129	Nile tilapia (Orechromis niloticus)	118:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	28	from	antioxidant	84:94	arg1	niloticus					143:151	Orechromis niloticus	132:151	Orechromis niloticus	132:151	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	3	29	from	effects	599:605	arg1	peroxidase					622:631	glutathione peroxidase	610:631	glutathione peroxidase	610:631	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	3	29	from	effects	599:605	arg1	growth					694:699	growth	694:699	growth	694:699	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	3	29	from	effects	599:605	arg1	composition					717:727	whole-body composition	706:727	whole-body composition of Nile tilapia	706:743	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	3	29	from	effects	599:605	arg1	activities					649:658	antioxidant activities	637:658	antioxidant activities	637:658	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	6	30	theme	Se-loaded	1402:1410	arg1	chitosan					1412:1419	Se-loaded chitosan	1402:1419	Se-loaded chitosan	1402:1419	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	5	31	theme	higher	1155:1160	arg1	activity					1174:1181	significantly higher antioxidant activity	1141:1181	significantly higher antioxidant activity	1141:1181	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	2	32	theme	controlled	500:509	arg1	profile					519:525	controlled release profile	500:525	controlled release profile	500:525	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	2	33	theme	activity	544:551	arg1	properties					553:562	antioxidant activity properties	532:562	antioxidant activity properties	532:562	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	3	34	theme	dietary	850:856	arg1	supplements					858:868	other selenium dietary supplements	835:868	other selenium dietary supplements	835:868	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	5	35	theme	Se-loaded	1107:1115	arg1	nanoparticles					1117:1129	Se-loaded nanoparticles	1107:1129	Se-loaded nanoparticles	1107:1129	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	10	36	from	activity	2008:2015	arg1	tilapia					2025:2031	Nile tilapia	2020:2031	Nile tilapia	2020:2031	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	4	37	theme	size	988:991	arg1	distribution					993:1004	broad size distribution	982:1004	broad size distribution	982:1004	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	0	38	theme	growth	100:105	arg1	effects					107:113	growth effects	100:113	growth effects	100:113	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	5	39	theme	controlled	1011:1020	arg1	release					1022:1028	The controlled release	1007:1028	The controlled release of Se	1007:1034	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	2	40	theme	ionotropic	430:439	arg1	method					450:455	the ionotropic gelation method	426:455	the ionotropic gelation method	426:455	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	0	41	theme	selenium	12:19	arg1	Delivery					0:7	Delivery	0:7	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).	0:153	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	6	42	dep	nanoparticles	1421:1433	arg1	Se-Nano					1436:1442	Se-Nano	1436:1442	Se-Nano	1436:1442	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	42	dep	nanoparticles	1421:1433	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	42	dep	nanoparticles	1421:1433	arg1	Se-Nano					1448:1454	Se-Nano	1448:1454	Se-Nano	1448:1454	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	0	43	theme	chitosan	27:34	arg1	nanoparticles					36:48	chitosan nanoparticles	27:48	chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus)	27:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	43	theme	chitosan	27:34	arg1	characterization					62:77	characterization	62:77	characterization	62:77	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	43	theme	chitosan	27:34	arg1	antioxidant					84:94	antioxidant	84:94	antioxidant	84:94	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	43	theme	chitosan	27:34	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis	51:59	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	1	44	theme	Oreochromis	285:295	arg1	tilapia					276:282	Nile tilapia	271:282	Nile tilapia (Oreochromis niloticus)	271:306	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	1	44	theme	Oreochromis	285:295	arg1	niloticus					297:305	Oreochromis niloticus	285:305	Oreochromis niloticus	285:305	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	7	45	theme	Se	1536:1537	arg1	supplementation					1539:1553	Se supplementation	1536:1553	Se supplementation	1536:1553	A basal diet without Se supplementation was used as the control.					
34003829	10	46	contain	containing	1853:1862	arg2	Se-Nano					1864:1870	Se-Nano	1864:1870	Se-Nano	1864:1870	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	10	46	contain	containing	1853:1862	arg1	Diets					1847:1851	Diets	1847:1851	Diets containing Se-Nano and Se-Nano x1.5	1847:1887	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	10	46	contain	containing	1853:1862	arg2	Se-Nano					1876:1882	Se-Nano x1.5	1876:1887	Se-Nano x1.5	1876:1887	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	3	47	theme	scavenging	672:681	arg1	activity					683:690	radical scavenging activity	664:690	radical scavenging activity	664:690	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	4	48	theme	Se-loaded	871:879	arg1	nanoparticles					890:902	Se-loaded chitosan nanoparticles	871:902	Se-loaded chitosan nanoparticles	871:902	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	10	49	theme	oxidative	1965:1973	arg1	stress					1975:1980	oxidative stress	1965:1980	oxidative stress	1965:1980	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	6	50	theme	tilapia	1506:1512	arg1	antioxidant					1465:1475	antioxidant	1465:1475	antioxidant	1465:1475	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	50	theme	tilapia	1506:1512	arg1	responses					1488:1496	growth responses	1481:1496	growth responses of Nile tilapia	1481:1512	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	1	51	theme	selenium-loaded	200:214	arg1	nanoparticles					225:237	selenium-loaded chitosan nanoparticles	200:237	selenium-loaded chitosan nanoparticles used as a dietary supplement	200:266	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	5	52	dep	h.	1104:1105	arg1	presented					1131:1139	presented	1131:1139	presented significantly higher antioxidant activity compared to free Se	1131:1201	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	1	53	theme	nanoparticles	225:237	arg1	responses					331:339	growth responses	324:339	growth responses	324:339	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	1	53	theme	nanoparticles	225:237	arg1	effects					189:195	the effects	185:195	the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant	185:318	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	11	54	theme	oxidative	2221:2229	arg1	stress					2231:2236	oxidative stress	2221:2236	oxidative stress	2221:2236	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	3	55	theme	Nile	732:735	arg1	tilapia					737:743	Nile tilapia	732:743	Nile tilapia	732:743	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	6	56	theme	growth	1481:1486	arg1	responses					1488:1496	growth responses	1481:1496	growth responses of Nile tilapia	1481:1512	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	5	57	theme	antioxidant	1162:1172	arg1	activity					1174:1181	significantly higher antioxidant activity	1141:1181	significantly higher antioxidant activity	1141:1181	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	4	58	theme	entrapment	916:925	arg1	efficiency					927:936	high entrapment efficiency	911:936	high entrapment efficiency (87%)	911:942	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	4	58	theme	entrapment	916:925	arg1	%					941:941	87%	939:941	87%	939:941	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	9	59	theme	dietary	1775:1781	arg1	treatments					1783:1792	dietary treatments	1775:1792	dietary treatments	1775:1792	However, dietary treatments did not affect whole-body protein and lipid content.					
34003829	0	60	dep	nanoparticles	36:48	arg1	nanoparticles					36:48	chitosan nanoparticles	27:48	chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus)	27:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	60	dep	nanoparticles	36:48	arg1	characterization					62:77	characterization	62:77	characterization	62:77	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	60	dep	nanoparticles	36:48	arg1	antioxidant					84:94	antioxidant	84:94	antioxidant	84:94	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	60	dep	nanoparticles	36:48	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis	51:59	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	8	61	theme	final	1704:1708	arg1	weight					1710:1715	final weight	1704:1715	final weight	1704:1715	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	1	62	theme	dietary	249:255	arg1	supplement					257:266	a dietary supplement	247:266	a dietary supplement	247:266	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	6	63	theme	feeding	1213:1219	arg1	trial					1221:1225	A 60-day feeding trial	1204:1225	A 60-day feeding trial	1204:1225	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	64	theme	sources	1302:1308	arg1	effects					1256:1262	the effects	1252:1262	the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia	1252:1512	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	4	65	theme	chitosan	881:888	arg1	nanoparticles					890:902	Se-loaded chitosan nanoparticles	871:902	Se-loaded chitosan nanoparticles	871:902	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	6	66	theme	dietary	1291:1297	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	66	theme	dietary	1291:1297	arg1	sources					1302:1308	supplementing different dietary Se sources	1267:1308	supplementing different dietary Se sources	1267:1308	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	0	67	theme	Nile	118:121	arg1	tilapia					123:129	Nile tilapia	118:129	Nile tilapia (Orechromis niloticus)	118:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	67	theme	Nile	118:121	arg1	niloticus					143:151	Orechromis niloticus	132:151	Orechromis niloticus	132:151	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	8	68	theme	Nile	1740:1743	arg1	fingerlings					1753:1763	Nile tilapia fingerlings	1740:1763	Nile tilapia fingerlings	1740:1763	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	6	69	theme	sodium	1359:1364	arg1	selenite					1366:1373	sodium selenite	1359:1373	sodium selenite (as inorganic source)	1359:1395	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	8	70	theme	fingerlings	1753:1763	arg1	efficiency					1726:1735	feed efficiency	1721:1735	feed efficiency	1721:1735	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	8	70	theme	fingerlings	1753:1763	arg1	weight					1710:1715	final weight	1704:1715	final weight	1704:1715	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	4	71	theme	smooth	962:967	arg1	surface					969:975	smooth surface	962:975	smooth surface	962:975	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	0	72	from	characterization	62:77	arg1	tilapia					123:129	Nile tilapia	118:129	Nile tilapia (Orechromis niloticus)	118:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	72	from	characterization	62:77	arg1	niloticus					143:151	Orechromis niloticus	132:151	Orechromis niloticus	132:151	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	73	from	effects	107:113	arg1	tilapia					123:129	Nile tilapia	118:129	Nile tilapia (Orechromis niloticus)	118:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	73	from	effects	107:113	arg1	niloticus					143:151	Orechromis niloticus	132:151	Orechromis niloticus	132:151	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	6	74	from	effects	1256:1262	arg1	antioxidant					1465:1475	antioxidant	1465:1475	antioxidant	1465:1475	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	74	from	effects	1256:1262	arg1	responses					1488:1496	growth responses	1481:1496	growth responses of Nile tilapia	1481:1512	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	3	75	theme	chitosan	794:801	arg1	nanoparticles					803:815	Se-loaded chitosan nanoparticles	784:815	Se-loaded chitosan nanoparticles	784:815	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	6	76	theme	organic	1342:1348	arg1	source					1350:1355	organic source	1342:1355	organic source	1342:1355	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	11	77	theme	dietary	2133:2139	arg1	Se					2141:2142	dietary Se	2133:2142	dietary Se	2133:2142	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	3	78	theme	selenium	841:848	arg1	supplements					858:868	other selenium dietary supplements	835:868	other selenium dietary supplements	835:868	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	6	79	theme	chitosan	1412:1419	arg1	Se-Nano					1436:1442	Se-Nano	1436:1442	Se-Nano	1436:1442	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	79	theme	chitosan	1412:1419	arg1	nanoparticles					1421:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles	1321:1433	selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5)	1321:1460	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	6	79	theme	chitosan	1412:1419	arg1	Se-Nano					1448:1454	Se-Nano	1448:1454	Se-Nano	1448:1454	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	5	80	theme	gradual	1080:1086	arg1	release					1088:1094	a gradual release	1078:1094	a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se	1078:1201	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	3	81	theme	antioxidant	637:647	arg1	activities					649:658	antioxidant activities	637:658	antioxidant activities	637:658	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	2	82	theme	release	511:517	arg1	profile					519:525	controlled release profile	500:525	controlled release profile	500:525	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	11	83	theme	antioxidant	2243:2253	arg1	activity					2255:2262	antioxidant activity	2243:2262	antioxidant activity	2243:2262	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	1	84	theme	growth	324:329	arg1	responses					331:339	growth responses	324:339	growth responses	324:339	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	8	85	dep	free	1636:1639	arg1	selenium					1658:1665	selenium	1658:1665	selenium	1658:1665	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	2	86	theme	antioxidant	532:542	arg1	properties					553:562	antioxidant activity properties	532:562	antioxidant activity properties	532:562	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	0	87	from	Synthesis	51:59	arg1	tilapia					123:129	Nile tilapia	118:129	Nile tilapia (Orechromis niloticus)	118:152	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	0	87	from	Synthesis	51:59	arg1	niloticus					143:151	Orechromis niloticus	132:151	Orechromis niloticus	132:151	Delivery of selenium using chitosan nanoparticles: Synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Orechromis niloticus).					
34003829	2	88	dep	First	342:346	arg1	chitosan-based					349:362	chitosan-based	349:362	chitosan-based	349:362	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	6	89	theme	inorganic	1379:1387	arg1	source					1389:1394	inorganic source	1379:1394	inorganic source	1379:1394	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	8	90	dep	sources	1627:1633	arg1	encapsulated					1645:1656	encapsulated	1645:1656	encapsulated	1645:1656	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	8	90	dep	sources	1627:1633	arg1	free					1636:1639	free	1636:1639	free	1636:1639	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	8	91	theme	significant	1676:1686	arg1	improvements					1688:1699	significant improvements	1676:1699	significant improvements in final weight and feed efficiency of Nile tilapia fingerlings	1676:1763	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	4	92	theme	broad	982:986	arg1	distribution					993:1004	broad size distribution	982:1004	broad size distribution	982:1004	Se-loaded chitosan nanoparticles showed high entrapment efficiency (87%), spherical shape, smooth surface, and broad size distribution.					
34003829	8	93	theme	different	1614:1622	arg1	sources					1627:1633	different Se sources	1614:1633	different Se sources (free and encapsulated selenium)	1614:1666	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	11	94	theme	species	2272:2278	arg1	efficiency					2209:2218	feed efficiency	2204:2218	feed efficiency	2204:2218	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	11	94	theme	species	2272:2278	arg1	stress					2231:2236	oxidative stress	2221:2236	oxidative stress	2221:2236	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	11	94	theme	species	2272:2278	arg1	activity					2255:2262	antioxidant activity	2243:2262	antioxidant activity	2243:2262	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	11	94	theme	species	2272:2278	arg1	performance					2191:2201	growth performance	2184:2201	growth performance	2184:2201	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	2	95	theme	gelation	441:448	arg1	method					450:455	the ionotropic gelation method	426:455	the ionotropic gelation method	426:455	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	1	96	theme	Nile	271:274	arg1	tilapia					276:282	Nile tilapia	271:282	Nile tilapia (Oreochromis niloticus)	271:306	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	1	96	theme	Nile	271:274	arg1	niloticus					297:305	Oreochromis niloticus	285:305	Oreochromis niloticus	285:305	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	11	97	theme	feed	2204:2207	arg1	efficiency					2209:2218	feed efficiency	2204:2218	feed efficiency	2204:2218	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	7	98	used	used	1559:1562	arg2	control					1571:1577	the control	1567:1577	the control	1567:1577	A basal diet without Se supplementation was used as the control.					
34003829	7	98	used	used	1559:1562	arg2	diet					1523:1526	A basal diet	1515:1526	A basal diet without Se supplementation	1515:1553	A basal diet without Se supplementation was used as the control.					
34003829	8	99	from	improvements	1688:1699	arg1	efficiency					1726:1735	feed efficiency	1721:1735	feed efficiency	1721:1735	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	8	99	from	improvements	1688:1699	arg1	weight					1710:1715	final weight	1704:1715	final weight	1704:1715	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	1	100	from	responses	331:339	arg1	tilapia					276:282	Nile tilapia	271:282	Nile tilapia (Oreochromis niloticus)	271:306	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	1	100	from	responses	331:339	arg1	niloticus					297:305	Oreochromis niloticus	285:305	Oreochromis niloticus	285:305	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	2	101	theme	physicochemical	467:481	arg1	characteristics					483:497	their physicochemical characteristics	461:497	their physicochemical characteristics	461:497	First, chitosan-based nanoparticles containing selenium (Se) were synthesized using the ionotropic gelation method and their physicochemical characteristics, controlled release profile, and antioxidant activity properties were investigated.					
34003829	8	102	theme	dietary	1584:1590	arg1	supplementations					1592:1607	The dietary supplementations	1580:1607	The dietary supplementations with different Se sources (free and encapsulated selenium)	1580:1666	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	3	103	theme	radical	664:670	arg1	activity					683:690	radical scavenging activity	664:690	radical scavenging activity	664:690	Thereafter, the effects on glutathione peroxidase and antioxidant activities (by radical scavenging activity), growth, and whole-body composition of Nile tilapia were evaluated when they were fed with Se-loaded chitosan nanoparticles and compared with other selenium dietary supplements.					
34003829	9	104	theme	whole-body	1809:1818	arg1	protein					1820:1826	whole-body protein and lipid content	1809:1844	protein	1820:1826	However, dietary treatments did not affect whole-body protein and lipid content.					
34003829	5	105	theme	free	1195:1198	arg1	Se					1200:1201	free Se	1195:1201	free Se	1195:1201	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	5	106	theme	initial	1052:1058	arg1	burst					1060:1064	an initial burst	1049:1064	an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se	1049:1201	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
34003829	11	107	theme	efficient	2101:2109	arg1	source					2111:2116	an efficient source	2098:2116	an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species	2098:2278	Overall, Se-loaded nanoparticles presented a great potential as an efficient source for delivering dietary Se to Nile tilapia, directly affecting the growth performance, feed efficiency, oxidative stress, and antioxidant activity of this species.					
34003829	10	108	theme	sodium	1914:1919	arg1	selenite					1921:1928	sodium selenite	1914:1928	sodium selenite	1914:1928	Diets containing Se-Nano and Se-Nano x1.5 were more effective than sodium selenite and selenomethionine in preventing oxidative stress and improving antioxidant activity in Nile tilapia.					
34003829	1	109	theme	antioxidant	308:318	arg1	effects					189:195	the effects	185:195	the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant	185:318	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	8	110	theme	Se	1624:1625	arg1	sources					1627:1633	different Se sources	1614:1633	different Se sources (free and encapsulated selenium)	1614:1666	The dietary supplementations with different Se sources (free and encapsulated selenium) lead to significant improvements in final weight and feed efficiency of Nile tilapia fingerlings.					
34003829	6	111	theme	Nile	1501:1504	arg1	tilapia					1506:1512	Nile tilapia	1501:1512	Nile tilapia	1501:1512	A 60-day feeding trial was conducted to compare the effects of supplementing different dietary Se sources, including selenomethionine (as organic source), sodium selenite (as inorganic source), and Se-loaded chitosan nanoparticles (Se-Nano and Se-Nano x1.5) on antioxidant and growth responses of Nile tilapia.					
34003829	1	112	theme	chitosan	216:223	arg1	nanoparticles					225:237	selenium-loaded chitosan nanoparticles	200:237	selenium-loaded chitosan nanoparticles used as a dietary supplement	200:266	This study aimed to elucidate the effects of selenium-loaded chitosan nanoparticles used as a dietary supplement on Nile tilapia (Oreochromis niloticus) antioxidant and growth responses.					
34003829	7	113	theme	basal	1517:1521	arg1	diet					1523:1526	A basal diet	1515:1526	A basal diet without Se supplementation	1515:1553	A basal diet without Se supplementation was used as the control.					
34003829	7	113	theme	basal	1517:1521	arg1	control					1571:1577	the control	1567:1577	the control	1567:1577	A basal diet without Se supplementation was used as the control.					
34003829	5	114	theme	Se	1033:1034	arg1	release					1022:1028	The controlled release	1007:1028	The controlled release of Se	1007:1034	The controlled release of Se consisted of an initial burst followed by a gradual release over 48 h. Se-loaded nanoparticles presented significantly higher antioxidant activity compared to free Se.					
31836652	5	0	theme	tracer	637:642	arg1	methodology					644:654	a chronic stable isotope tracer methodology	612:654	a chronic stable isotope tracer methodology	612:654	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	10	1	theme	seasonal	1388:1395	arg1	effect					1397:1402	a seasonal effect	1386:1402	a seasonal effect on fat turnover rate	1386:1423	We also found a seasonal effect on fat turnover rate.					
31836652	2	2	theme	future	315:320	arg1	periods					330:336	future fasting periods	315:336	future fasting periods	315:336	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	12	3	theme	sucrose	1744:1750	arg1	partitioning					1728:1739	the partitioning	1724:1739	the partitioning of sucrose towards de novo fatty acid synthesis	1724:1787	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	4	4	theme	fat	582:584	arg1	storage					586:592	fat storage	582:592	fat storage	582:592	Whether and how hummingbirds partition dietary fructose and glucose towards immediate oxidation versus fat storage is unknown.					
31836652	6	5	theme	Potential	798:806	arg1	changes					817:823	Potential seasonal changes	798:823	Potential seasonal changes	798:823	Potential seasonal changes were correlated with variation in the overall daily energy expenditure.					
31836652	2	6	theme	immediate	260:268	arg1	energy					270:275	immediate energy needs	260:281	immediate energy needs	260:281	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	12	7	theme	de	1760:1761	arg1	synthesis					1779:1787	de novo fatty acid synthesis	1760:1787	de novo fatty acid synthesis	1760:1787	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	10	8	theme	turnover	1411:1418	arg1	rate					1420:1423	fat turnover rate	1407:1423	fat turnover rate	1407:1423	We also found a seasonal effect on fat turnover rate.					
31836652	6	9	theme	energy	877:882	arg1	expenditure					884:894	the overall daily energy expenditure	859:894	the overall daily energy expenditure	859:894	Potential seasonal changes were correlated with variation in the overall daily energy expenditure.					
31836652	5	10	theme	stable	622:627	arg1	methodology					644:654	a chronic stable isotope tracer methodology	612:654	a chronic stable isotope tracer methodology	612:654	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	9	11	theme	isotopic	1213:1220	arg1	enrichment					1222:1231	greater isotopic enrichment	1205:1231	greater isotopic enrichment of fat stores	1205:1245	We found greater isotopic enrichment of fat stores when glucose was labelled compared with fructose, suggesting preference for glucose as a substrate for fatty acid synthesis.					
31836652	11	12	theme	fat	1522:1524	arg1	mass					1516:1519	lower body mass	1505:1519	lower body mass	1505:1519	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	11	12	theme	fat	1522:1524	arg1	stores					1526:1531	fat stores	1522:1531	fat stores	1522:1531	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	0	13	from	rate	71:74	arg1	hummingbirds					93:104	ruby-throated hummingbirds	79:104	ruby-throated hummingbirds (Archilochus colubris)	79:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	9	14	theme	stores	1240:1245	arg1	enrichment					1222:1231	greater isotopic enrichment	1205:1231	greater isotopic enrichment of fat stores	1205:1245	We found greater isotopic enrichment of fat stores when glucose was labelled compared with fructose, suggesting preference for glucose as a substrate for fatty acid synthesis.					
31836652	7	15	theme	disaccharide	1022:1033	arg1	portion					1007:1013	the fructose portion	994:1013	the fructose portion of the disaccharide for 5 days	994:1044	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	7	15	theme	disaccharide	1022:1033	arg1	glucose					983:989	the glucose	979:989	the glucose	979:989	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	5	16	dep	de	724:725	arg1	novo					727:730	novo	727:730	novo	727:730	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	5	17	from	lipogenesis	732:742	arg1	hummingbirds					761:772	ruby-throated hummingbirds	747:772	ruby-throated hummingbirds (Archilochus colubris)	747:795	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	7	18	theme	fructose	998:1005	arg1	portion					1007:1013	the fructose portion	994:1013	the fructose portion of the disaccharide for 5 days	994:1044	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	0	19	theme	fat	58:60	arg1	rate					71:74	fat turnover rate	58:74	fat turnover rate in ruby-throated hummingbirds (Archilochus colubris)	58:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	12	20	theme	energy	1674:1679	arg1	expenditure					1681:1691	energy expenditure	1674:1691	energy expenditure	1674:1691	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	2	21	dep	energy	270:275	arg1	needs					277:281	needs	277:281	needs	277:281	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	11	22	theme	body	1511:1514	arg1	mass					1516:1519	lower body mass	1505:1519	lower body mass	1505:1519	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	11	22	theme	body	1511:1514	arg1	stores					1526:1531	fat stores	1522:1531	fat stores	1522:1531	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	0	23	theme	ruby-throated	79:91	arg1	hummingbirds					93:104	ruby-throated hummingbirds	79:104	ruby-throated hummingbirds (Archilochus colubris)	79:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	8	24	theme	Isotopic	1047:1054	arg1	incorporation					1056:1068	Isotopic incorporation	1047:1068	Isotopic incorporation into fat stores	1047:1084	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	0	25	from	changes	47:53	arg1	rate					71:74	fat turnover rate	58:74	fat turnover rate in ruby-throated hummingbirds (Archilochus colubris)	58:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	11	26	theme	turnover	1433:1440	arg1	rates					1442:1446	Faster turnover rates	1426:1446	Faster turnover rates	1426:1446	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	0	27	theme	Metabolic	0:8	arg1	partitioning					10:21	Metabolic partitioning	0:21	Metabolic partitioning of sucrose	0:32	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	12	28	theme	fat	1619:1621	arg1	rate					1632:1635	fat turnover rate	1619:1635	fat turnover rate	1619:1635	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	3	29	theme	Fasting	339:345	arg1	hummingbirds					347:358	Fasting hummingbirds	339:358	Fasting hummingbirds	339:358	Fasting hummingbirds can deplete energy stores in only hours and need to be continuously replacing these stores while feeding and foraging.					
31836652	6	30	theme	daily	871:875	arg1	expenditure					884:894	the overall daily energy expenditure	859:894	the overall daily energy expenditure	859:894	Potential seasonal changes were correlated with variation in the overall daily energy expenditure.					
31836652	9	31	theme	acid	1356:1359	arg1	synthesis					1361:1369	fatty acid synthesis	1350:1369	fatty acid synthesis	1350:1369	We found greater isotopic enrichment of fat stores when glucose was labelled compared with fructose, suggesting preference for glucose as a substrate for fatty acid synthesis.					
31836652	3	32	theme	energy	372:377	arg1	stores					379:384	energy stores	372:384	energy stores in only hours	372:398	Fasting hummingbirds can deplete energy stores in only hours and need to be continuously replacing these stores while feeding and foraging.					
31836652	8	33	theme	13C	1114:1116	arg1	signature					1118:1126	the breath 13C signature	1103:1126	the breath 13C signature while fasting (oxidizing fat)	1103:1156	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	11	34	theme	higher	1547:1552	arg1	intake					1567:1572	higher daily nectar intake	1547:1572	higher daily nectar intake	1547:1572	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	7	35	theme	sucrose-based	931:943	arg1	diets					945:949	ruby-throated hummingbirds sucrose-based diets	904:949	ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days	904:1044	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	1	36	theme	nectar	211:216	arg1	diets					218:222	their nectar diets	205:222	their nectar diets	205:222	Hummingbirds fuel their high energy needs with the fructose and glucose in their nectar diets.					
31836652	12	37	theme	acid	1774:1777	arg1	synthesis					1779:1787	de novo fatty acid synthesis	1760:1787	de novo fatty acid synthesis	1760:1787	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	7	38	theme	ruby-throated	904:916	arg1	diets					945:949	ruby-throated hummingbirds sucrose-based diets	904:949	ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days	904:1044	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	10	39	from	effect	1397:1402	arg1	rate					1420:1423	fat turnover rate	1407:1423	fat turnover rate	1407:1423	We also found a seasonal effect on fat turnover rate.					
31836652	5	40	theme	isotope	629:635	arg1	methodology					644:654	a chronic stable isotope tracer methodology	612:654	a chronic stable isotope tracer methodology	612:654	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	2	41	theme	fasting	322:328	arg1	periods					330:336	future fasting periods	315:336	future fasting periods	315:336	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	4	42	theme	immediate	555:563	arg1	oxidation					565:573	immediate oxidation	555:573	immediate oxidation	555:573	Whether and how hummingbirds partition dietary fructose and glucose towards immediate oxidation versus fat storage is unknown.					
31836652	11	43	theme	daily	1554:1558	arg1	intake					1567:1572	higher daily nectar intake	1547:1572	higher daily nectar intake	1547:1572	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	6	44	theme	seasonal	808:815	arg1	changes					817:823	Potential seasonal changes	798:823	Potential seasonal changes	798:823	Potential seasonal changes were correlated with variation in the overall daily energy expenditure.					
31836652	12	45	dep	de	1760:1761	arg1	novo					1763:1766	novo	1763:1766	novo	1763:1766	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	2	46	used	used	242:245	arg2	sugars					231:236	These sugars	225:236	These sugars	225:236	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	5	47	theme	chronic	614:620	arg1	methodology					644:654	a chronic stable isotope tracer methodology	612:654	a chronic stable isotope tracer methodology	612:654	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	9	48	theme	greater	1205:1211	arg1	enrichment					1222:1231	greater isotopic enrichment	1205:1231	greater isotopic enrichment of fat stores	1205:1245	We found greater isotopic enrichment of fat stores when glucose was labelled compared with fructose, suggesting preference for glucose as a substrate for fatty acid synthesis.					
31836652	2	49	dep	used	242:245	arg1	fuel					255:258	fuel	255:258	to fuel immediate energy needs	252:281	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	2	49	dep	used	242:245	arg1	build					290:294	build	290:294	to build fat stores to fuel future fasting periods	287:336	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	5	50	dep	hummingbirds	761:772	arg1	colubris					787:794	Archilochus colubris	775:794	Archilochus colubris	775:794	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	9	51	theme	fat	1236:1238	arg1	stores					1240:1245	fat stores	1236:1245	fat stores	1236:1245	We found greater isotopic enrichment of fat stores when glucose was labelled compared with fructose, suggesting preference for glucose as a substrate for fatty acid synthesis.					
31836652	6	52	from	variation	846:854	arg1	expenditure					884:894	the overall daily energy expenditure	859:894	the overall daily energy expenditure	859:894	Potential seasonal changes were correlated with variation in the overall daily energy expenditure.					
31836652	1	53	from	glucose	194:200	arg1	diets					218:222	their nectar diets	205:222	their nectar diets	205:222	Hummingbirds fuel their high energy needs with the fructose and glucose in their nectar diets.					
31836652	12	54	theme	body	1697:1700	arg1	composition					1702:1712	body composition	1697:1712	body composition	1697:1712	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	7	55	from	13C	965:967	arg1	portion					1007:1013	the fructose portion	994:1013	the fructose portion of the disaccharide for 5 days	994:1044	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	7	55	from	13C	965:967	arg1	glucose					983:989	the glucose	979:989	the glucose	979:989	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	11	56	theme	summer	1468:1473	arg1	months					1475:1480	the summer months	1464:1480	the summer months	1464:1480	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	0	57	theme	turnover	62:69	arg1	rate					71:74	fat turnover rate	58:74	fat turnover rate in ruby-throated hummingbirds (Archilochus colubris)	58:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	1	58	from	fructose	181:188	arg1	diets					218:222	their nectar diets	205:222	their nectar diets	205:222	Hummingbirds fuel their high energy needs with the fructose and glucose in their nectar diets.					
31836652	11	59	theme	lower	1505:1509	arg1	mass					1516:1519	lower body mass	1505:1519	lower body mass	1505:1519	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	11	59	theme	lower	1505:1509	arg1	stores					1526:1531	fat stores	1522:1531	fat stores	1522:1531	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	5	60	theme	ruby-throated	747:759	arg1	hummingbirds					761:772	ruby-throated hummingbirds	747:772	ruby-throated hummingbirds (Archilochus colubris)	747:795	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	3	61	from	stores	379:384	arg1	hours					394:398	only hours	389:398	only hours	389:398	Fasting hummingbirds can deplete energy stores in only hours and need to be continuously replacing these stores while feeding and foraging.					
31836652	1	62	theme	high	154:157	arg1	energy					159:164	their high energy needs	148:170	their high energy needs	148:170	Hummingbirds fuel their high energy needs with the fructose and glucose in their nectar diets.					
31836652	0	63	from	partitioning	10:21	arg1	rate					71:74	fat turnover rate	58:74	fat turnover rate in ruby-throated hummingbirds (Archilochus colubris)	58:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	8	64	dep	winter	1169:1174	arg1	the					1165:1167	the	1165:1167	the	1165:1167	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	8	64	dep	winter	1169:1174	arg1	seasons					1187:1193	seasons	1187:1193	seasons	1187:1193	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	5	65	used	used	715:718	arg2	fructose					688:695	fructose	688:695	fructose	688:695	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	5	65	used	used	715:718	arg2	glucose					677:683	glucose	677:683	glucose	677:683	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	11	66	theme	Faster	1426:1431	arg1	rates					1442:1446	Faster turnover rates	1426:1446	Faster turnover rates	1426:1446	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	5	67	theme	de	724:725	arg1	lipogenesis					732:742	de novo lipogenesis	724:742	de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris)	724:795	Using a chronic stable isotope tracer methodology, we examined whether glucose or fructose is preferentially used for de novo lipogenesis in ruby-throated hummingbirds (Archilochus colubris).					
31836652	8	68	theme	fat	1075:1077	arg1	stores					1079:1084	fat stores	1075:1084	fat stores	1075:1084	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	0	69	dep	hummingbirds	93:104	arg1	colubris					119:126	Archilochus colubris	107:126	Archilochus colubris	107:126	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	1	70	dep	energy	159:164	arg1	needs					166:170	needs	166:170	needs	166:170	Hummingbirds fuel their high energy needs with the fructose and glucose in their nectar diets.					
31836652	0	71	theme	sucrose	26:32	arg1	changes					47:53	seasonal changes	38:53	seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris)	38:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	0	71	theme	sucrose	26:32	arg1	partitioning					10:21	Metabolic partitioning	0:21	Metabolic partitioning of sucrose	0:32	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	10	72	theme	fat	1407:1409	arg1	rate					1420:1423	fat turnover rate	1407:1423	fat turnover rate	1407:1423	We also found a seasonal effect on fat turnover rate.					
31836652	0	73	theme	seasonal	38:45	arg1	changes					47:53	seasonal changes	38:53	seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris)	38:127	Metabolic partitioning of sucrose and seasonal changes in fat turnover rate in ruby-throated hummingbirds (Archilochus colubris).					
31836652	9	74	theme	fatty	1350:1354	arg1	synthesis					1361:1369	fatty acid synthesis	1350:1369	fatty acid synthesis	1350:1369	We found greater isotopic enrichment of fat stores when glucose was labelled compared with fructose, suggesting preference for glucose as a substrate for fatty acid synthesis.					
31836652	8	75	theme	breath	1107:1112	arg1	signature					1118:1126	the breath 13C signature	1103:1126	the breath 13C signature while fasting (oxidizing fat)	1103:1156	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	6	76	theme	overall	863:869	arg1	expenditure					884:894	the overall daily energy expenditure	859:894	the overall daily energy expenditure	859:894	Potential seasonal changes were correlated with variation in the overall daily energy expenditure.					
31836652	12	77	theme	turnover	1623:1630	arg1	rate					1632:1635	fat turnover rate	1619:1635	fat turnover rate	1619:1635	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
31836652	3	78	theme	only	389:392	arg1	hours					394:398	only hours	389:398	only hours	389:398	Fasting hummingbirds can deplete energy stores in only hours and need to be continuously replacing these stores while feeding and foraging.					
31836652	1	79	dep	fructose	181:188	arg1	the					177:179	the	177:179	the	177:179	Hummingbirds fuel their high energy needs with the fructose and glucose in their nectar diets.					
31836652	4	80	dep	Whether	479:485	arg1	partition					508:516	partition	508:516	partition	508:516	Whether and how hummingbirds partition dietary fructose and glucose towards immediate oxidation versus fat storage is unknown.					
31836652	4	81	theme	dietary	518:524	arg1	fructose					526:533	dietary fructose	518:533	dietary fructose	518:533	Whether and how hummingbirds partition dietary fructose and glucose towards immediate oxidation versus fat storage is unknown.					
31836652	11	82	theme	nectar	1560:1565	arg1	intake					1567:1572	higher daily nectar intake	1547:1572	higher daily nectar intake	1547:1572	Faster turnover rates occurred during the summer months, when birds maintained lower body mass, fat stores and exhibited higher daily nectar intake compared with winter.					
31836652	7	83	theme	hummingbirds	918:929	arg1	diets					945:949	ruby-throated hummingbirds sucrose-based diets	904:949	ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days	904:1044	We fed ruby-throated hummingbirds sucrose-based diets enriched with 13C on either the glucose or the fructose portion of the disaccharide for 5 days.					
31836652	2	84	theme	fat	296:298	arg1	stores					300:305	fat stores	296:305	fat stores	296:305	These sugars are used both to fuel immediate energy needs and to build fat stores to fuel future fasting periods.					
31836652	8	85	theme	oxidizing	1143:1151	arg1	fat					1153:1155	oxidizing fat	1143:1155	oxidizing fat	1143:1155	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	8	85	theme	oxidizing	1143:1151	arg1	fasting					1134:1140	fasting	1134:1140	fasting (oxidizing fat)	1134:1156	Isotopic incorporation into fat stores was measured via the breath 13C signature while fasting (oxidizing fat) during the winter and summer seasons.					
31836652	12	86	theme	fatty	1768:1772	arg1	synthesis					1779:1787	de novo fatty acid synthesis	1760:1787	de novo fatty acid synthesis	1760:1787	This demonstrates that fat turnover rate can substantially vary with changing energy expenditure and body composition; however, the partitioning of sucrose towards de novo fatty acid synthesis remains constant.					
33476614	0	0	theme	hydroxypropyl	80:92	arg1	cellulose					101:109	hydroxypropyl methyl cellulose	80:109	hydroxypropyl methyl cellulose	80:109	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	5	1	theme	composite	731:739	arg1	film					741:744	the composite film	727:744	the composite film	727:744	The best antioxidant and antibacterial properties were obtained when the addition of MBE in the composite film was 20%.					
33476614	1	2	theme	packaging	167:175	arg1	film					177:180	A pH-sensitive food packaging film	147:180	A pH-sensitive food packaging film	147:180	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	4	3	theme	KH-MBE-20	556:564	arg1	film					567:570	the KH-MBE-20% film	552:570	the KH-MBE-20% film	552:570	Notably, the KH-MBE-20% film almost completely blocked UV light in the range of 200-600 nm.					
33476614	6	4	contain	has	780:782	arg1	film					775:778	KH-MBE film	768:778	KH-MBE film	768:778	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	6	4	contain	has	780:782	arg2	responsiveness					789:802	good responsiveness	784:802	good responsiveness to buffers with pH range from 2 to 12	784:840	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	6	5	theme	KH-MBE	768:773	arg1	film					775:778	KH-MBE film	768:778	KH-MBE film	768:778	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	6	6	dep	12	839:840	arg1	to					836:837	to	836:837	to	836:837	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	0	7	theme	methyl	94:99	arg1	cellulose					101:109	hydroxypropyl methyl cellulose	80:109	hydroxypropyl methyl cellulose	80:109	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	7	8	contain	had	1037:1039	arg2	stability					1056:1064	the best color stability	1041:1064	the best color stability	1041:1064	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	7	8	contain	had	1037:1039	arg1	%					1030:1030	the KH-MBE-20% film	1017:1035	the KH-MBE-20% film	1017:1035	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	3	9	theme	UV	528:529	arg1	resistance					531:540	UV resistance	528:540	UV resistance	528:540	The incorporation of MBE into KGM and HPMC (KH) films can significantly improve the mechanical properties and UV resistance.					
33476614	4	10	theme	%	565:565	arg1	film					567:570	the KH-MBE-20% film	552:570	the KH-MBE-20% film	552:570	Notably, the KH-MBE-20% film almost completely blocked UV light in the range of 200-600 nm.					
33476614	8	11	theme	freshness	1174:1182	arg1	monitoring					1155:1164	real-time monitoring	1145:1164	real-time monitoring of fish freshness	1145:1182	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	3	12	theme	mechanical	502:511	arg1	properties					513:522	the mechanical properties	498:522	the mechanical properties	498:522	The incorporation of MBE into KGM and HPMC (KH) films can significantly improve the mechanical properties and UV resistance.					
33476614	4	13	theme	200-600 nm	623:632	arg1	range					614:618	the range	610:618	the range of 200-600 nm	610:632	Notably, the KH-MBE-20% film almost completely blocked UV light in the range of 200-600 nm.					
33476614	5	14	theme	MBE	720:722	arg1	%					752:752	20%	750:752	20%	750:752	The best antioxidant and antibacterial properties were obtained when the addition of MBE in the composite film was 20%.					
33476614	5	14	theme	MBE	720:722	arg1	addition					708:715	the addition	704:715	the addition of MBE in the composite film	704:744	The best antioxidant and antibacterial properties were obtained when the addition of MBE in the composite film was 20%.					
33476614	7	15	theme	fish	997:1000	arg1	freshness					980:988	the freshness	976:988	the freshness of the fish	976:1000	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	3	16	theme	MBE	439:441	arg1	incorporation					422:434	The incorporation	418:434	The incorporation of MBE into KGM and HPMC (KH) films	418:470	The incorporation of MBE into KGM and HPMC (KH) films can significantly improve the mechanical properties and UV resistance.					
33476614	7	17	theme	color	1050:1054	arg1	stability					1056:1064	the best color stability	1041:1064	the best color stability	1041:1064	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	8	18	contain	has	1115:1117	arg1	packaging					1090:1098	intelligent packaging	1078:1098	intelligent packaging of KH-MBE film	1078:1113	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	8	18	contain	has	1115:1117	arg2	applications					1129:1140	potential applications	1119:1140	potential applications	1119:1140	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	2	19	theme	molecular	367:375	arg1	interactions					377:388	good molecular interactions	362:388	good molecular interactions among the three components	362:415	FT-IR and XRD analysis revealed that there are good molecular interactions among the three components.					
33476614	8	20	theme	potential	1119:1127	arg1	applications					1129:1140	potential applications	1119:1140	potential applications	1119:1140	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	7	21	theme	monitoring	853:862	arg1	experiments					864:874	visual monitoring experiments	846:874	visual monitoring experiments using the film on fresh fish	846:903	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	8	22	theme	real-time	1145:1153	arg1	monitoring					1155:1164	real-time monitoring	1145:1164	real-time monitoring of fish freshness	1145:1182	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	2	23	theme	good	362:365	arg1	interactions					377:388	good molecular interactions	362:388	good molecular interactions among the three components	362:415	FT-IR and XRD analysis revealed that there are good molecular interactions among the three components.					
33476614	0	24	theme	food	28:31	arg1	film					43:46	pH-sensitive food packaging film	15:46	pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose	15:109	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	8	25	theme	intelligent	1078:1088	arg1	packaging					1090:1098	intelligent packaging	1078:1098	intelligent packaging of KH-MBE film	1078:1113	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	7	26	theme	fresh	894:898	arg1	fish					900:903	fresh fish	894:903	fresh fish	894:903	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	1	27	theme	konjac	204:209	arg1	glucomannan					211:221	konjac glucomannan	204:221	konjac glucomannan (KGM)	204:227	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	1	27	theme	konjac	204:209	arg1	KGM					224:226	KGM	224:226	KGM	224:226	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	0	28	theme	pH-sensitive	15:26	arg1	film					43:46	pH-sensitive food packaging film	15:46	pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose	15:109	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	1	29	theme	mulberry	289:296	arg1	MBE					309:311	MBE	309:311	MBE	309:311	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	1	29	theme	mulberry	289:296	arg1	extracts2					298:306	mulberry extracts2	289:306	mulberry extracts2 (MBE)	289:312	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	0	30	theme	mulberry	129:136	arg1	extract					138:144	mulberry extract	129:144	mulberry extract	129:144	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	6	31	theme	pH	820:821	arg1	range					823:827	pH range	820:827	pH range from 2 to 12	820:840	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	7	32	from	film	886:889	arg1	fish					900:903	fresh fish	894:903	fresh fish	894:903	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	3	33	theme	HPMC	456:459	arg1	films					466:470	KGM and HPMC (KH) films	448:470	KGM and HPMC (KH) films	448:470	The incorporation of MBE into KGM and HPMC (KH) films can significantly improve the mechanical properties and UV resistance.					
33476614	0	34	theme	film	43:46	arg1	Preparation					0:10	Preparation	0:10	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose	0:109	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	6	35	with	buffers	807:813	arg1	range					823:827	pH range	820:827	pH range from 2 to 12	820:840	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	8	36	theme	fish	1169:1172	arg1	freshness					1174:1182	fish freshness	1169:1182	fish freshness	1169:1182	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	0	37	theme	packaging	33:41	arg1	film					43:46	pH-sensitive food packaging film	15:46	pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose	15:109	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	4	38	theme	UV	598:599	arg1	light					601:605	UV light	598:605	UV light	598:605	Notably, the KH-MBE-20% film almost completely blocked UV light in the range of 200-600 nm.					
33476614	7	39	theme	film	930:933	arg1	color					910:914	the color	906:914	the color of the KH-MBE film	906:933	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	3	40	theme	KGM	448:450	arg1	films					466:470	KGM and HPMC (KH) films	448:470	KGM and HPMC (KH) films	448:470	The incorporation of MBE into KGM and HPMC (KH) films can significantly improve the mechanical properties and UV resistance.					
33476614	8	41	theme	film	1110:1113	arg1	packaging					1090:1098	intelligent packaging	1078:1098	intelligent packaging of KH-MBE film	1078:1113	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	5	42	from	addition	708:715	arg1	film					741:744	the composite film	727:744	the composite film	727:744	The best antioxidant and antibacterial properties were obtained when the addition of MBE in the composite film was 20%.					
33476614	8	43	theme	KH-MBE	1103:1108	arg1	film					1110:1113	KH-MBE film	1103:1113	KH-MBE film	1103:1113	Therefore, intelligent packaging of KH-MBE film has potential applications in real-time monitoring of fish freshness.					
33476614	2	44	theme	XRD	325:327	arg1	analysis					329:336	FT-IR and XRD analysis	315:336	FT-IR and XRD analysis	315:336	FT-IR and XRD analysis revealed that there are good molecular interactions among the three components.					
33476614	6	45	from	12	839:840	arg1	range					823:827	pH range	820:827	pH range from 2 to 12	820:840	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	6	46	theme	good	784:787	arg1	responsiveness					789:802	good responsiveness	784:802	good responsiveness to buffers with pH range from 2 to 12	784:840	In addition, KH-MBE film has good responsiveness to buffers with pH range from 2 to 12.					
33476614	7	47	theme	KH-MBE	923:928	arg1	film					930:933	the KH-MBE film	919:933	the KH-MBE film	919:933	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	7	48	theme	visual	846:851	arg1	experiments					864:874	visual monitoring experiments	846:874	visual monitoring experiments using the film on fresh fish	846:903	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	5	49	theme	antibacterial	660:672	arg1	properties					674:683	antibacterial properties	660:683	antibacterial properties	660:683	The best antioxidant and antibacterial properties were obtained when the addition of MBE in the composite film was 20%.					
33476614	2	50	theme	FT-IR	315:319	arg1	analysis					329:336	FT-IR and XRD analysis	315:336	FT-IR and XRD analysis	315:336	FT-IR and XRD analysis revealed that there are good molecular interactions among the three components.					
33476614	7	51	dep	gray	958:961	arg1	purple					948:953	purple	948:953	purple	948:953	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	1	52	theme	pH-sensitive	149:160	arg1	film					177:180	A pH-sensitive food packaging film	147:180	A pH-sensitive food packaging film	147:180	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	1	53	theme	hydroxypropyl	233:245	arg1	HPMC					265:268	HPMC	265:268	HPMC	265:268	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	1	53	theme	hydroxypropyl	233:245	arg1	cellulose					254:262	hydroxypropyl methyl cellulose	233:262	hydroxypropyl methyl cellulose (HPMC)	233:269	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	0	54	theme	konjac	57:62	arg1	glucomannan					64:74	konjac glucomannan	57:74	konjac glucomannan	57:74	Preparation of pH-sensitive food packaging film based on konjac glucomannan and hydroxypropyl methyl cellulose incorporated with mulberry extract.					
33476614	7	55	theme	best	1045:1048	arg1	stability					1056:1064	the best color stability	1041:1064	the best color stability	1041:1064	In visual monitoring experiments using the film on fresh fish, the color of the KH-MBE film changed from purple to gray to yellow as the freshness of the fish decreased, and the KH-MBE-20% film had the best color stability.					
33476614	1	56	theme	food	162:165	arg1	film					177:180	A pH-sensitive food packaging film	147:180	A pH-sensitive food packaging film	147:180	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	1	57	theme	methyl	247:252	arg1	HPMC					265:268	HPMC	265:268	HPMC	265:268	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
33476614	1	57	theme	methyl	247:252	arg1	cellulose					254:262	hydroxypropyl methyl cellulose	233:262	hydroxypropyl methyl cellulose (HPMC)	233:269	A pH-sensitive food packaging film was prepared based on konjac glucomannan (KGM) and hydroxypropyl methyl cellulose (HPMC) incorporated with mulberry extracts2 (MBE).					
32564842	1	0	theme	matrix	350:355	arg1	compatibility					314:326	compatibility	314:326	compatibility	314:326	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	1	0	theme	matrix	350:355	arg1	properties					332:341	properties	332:341	properties	332:341	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	2	1	theme	TPS/PBAT/HNTs	358:370	arg1	nano-biocomposites					372:389	TPS/PBAT/HNTs nano-biocomposites	358:389	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents	358:448	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	0	2	theme	halloysite	103:112	arg1	nanoclay					114:121	halloysite nanoclay	103:121	halloysite nanoclay	103:121	Morphology and properties of thermoplastic starch blended with biodegradable polyester and filled with halloysite nanoclay.					
32564842	5	3	theme	HNTs	1021:1024	arg1	incorporation					995:1007	The incorporation	991:1007	The incorporation of 5 wt% of HNTs	991:1024	The incorporation of 5 wt% of HNTs improved compatibility and increased Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends approx.					
32564842	3	4	dep	exhibited	593:601	arg1	whereas					668:674	whereas	668:674	whereas	668:674	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	4	5	theme	biocomposites	780:792	arg1	Elongation					739:748	Elongation	739:748	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs	739:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	1	6	theme	thermoplastic	183:195	arg1	starch/poly					197:207	thermoplastic starch/poly	183:207	thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends	183:260	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	1	6	theme	thermoplastic	183:195	arg1	adipate-co-terephthalate					218:241	butylene adipate-co-terephthalate	209:241	butylene adipate-co-terephthalate	209:241	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	1	6	theme	thermoplastic	183:195	arg1	TPS/PBAT					245:252	TPS/PBAT	245:252	TPS/PBAT	245:252	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	3	7	theme	TPS20/PBAT80	573:584	arg1	blends					586:591	The TPS80/PBAT20 and TPS20/PBAT80 blends	552:591	The TPS80/PBAT20 and TPS20/PBAT80 blends	552:591	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	2	8	theme	blending	479:486	arg1	process					488:494	a melt blending process	472:494	a melt blending process	472:494	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	1	9	dep	compatibility	314:326	arg1	the					310:312	the	310:312	the	310:312	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	7	10	theme	promising	1224:1232	arg1	HNTs					1215:1218	HNTs	1215:1218	HNTs	1215:1218	These results demonstrate that HNTs are promising nanofillers to improve properties of TPS-based blends.					
32564842	7	10	theme	promising	1224:1232	arg1	nanofillers					1234:1244	promising nanofillers	1224:1244	promising nanofillers	1224:1244	These results demonstrate that HNTs are promising nanofillers to improve properties of TPS-based blends.					
32564842	2	11	theme	weight	415:420	arg1	fractions					422:430	different TPS/PBAT weight fractions	396:430	different TPS/PBAT weight fractions	396:430	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	2	12	theme	melt	474:477	arg1	process					488:494	a melt blending process	472:494	a melt blending process	472:494	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	3	13	theme	small	623:627	arg1	droplets					629:636	small droplets	623:636	small droplets of PBAT or TPS	623:651	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	2	14	theme	TPS/PBAT	406:413	arg1	fractions					422:430	different TPS/PBAT weight fractions	396:430	different TPS/PBAT weight fractions	396:430	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	4	15	dep	%	880:880	arg1	i.e.					870:873	i.e.	870:873	i.e.	870:873	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	4	16	theme	PBAT	853:856	arg1	proportion					858:867	PBAT proportion	853:867	PBAT proportion	853:867	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	3	17	theme	dispersed	603:611	arg1	phases					613:618	dispersed phases	603:618	dispersed phases of small droplets of PBAT or TPS	603:651	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	5	18	theme	%	1016:1016	arg1	incorporation					995:1007	The incorporation	991:1007	The incorporation of 5 wt% of HNTs	991:1024	The incorporation of 5 wt% of HNTs improved compatibility and increased Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends approx.					
32564842	7	19	theme	blends	1281:1286	arg1	properties					1257:1266	properties	1257:1266	properties of TPS-based blends	1257:1286	These results demonstrate that HNTs are promising nanofillers to improve properties of TPS-based blends.					
32564842	3	20	theme	TPS80/PBAT20	556:567	arg1	blends					586:591	The TPS80/PBAT20 and TPS20/PBAT80 blends	552:591	The TPS80/PBAT20 and TPS20/PBAT80 blends	552:591	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	3	21	theme	TPS50/PBAT50	680:691	arg1	blend					693:697	the TPS50/PBAT50 blend	676:697	the TPS50/PBAT50 blend	676:697	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	1	22	theme	butylene	209:216	arg1	starch/poly					197:207	thermoplastic starch/poly	183:207	thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends	183:260	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	1	22	theme	butylene	209:216	arg1	adipate-co-terephthalate					218:241	butylene adipate-co-terephthalate	209:241	butylene adipate-co-terephthalate	209:241	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	0	23	theme	starch	43:48	arg1	properties					15:24	properties	15:24	properties	15:24	Morphology and properties of thermoplastic starch blended with biodegradable polyester and filled with halloysite nanoclay.					
32564842	0	23	theme	starch	43:48	arg1	Morphology					0:9	Morphology	0:9	Morphology	0:9	Morphology and properties of thermoplastic starch blended with biodegradable polyester and filled with halloysite nanoclay.					
32564842	7	24	theme	TPS-based	1271:1279	arg1	blends					1281:1286	TPS-based blends	1271:1286	TPS-based blends	1271:1286	These results demonstrate that HNTs are promising nanofillers to improve properties of TPS-based blends.					
32564842	3	25	theme	PBAT	641:644	arg1	droplets					629:636	small droplets	623:636	small droplets of PBAT or TPS	623:651	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	1	26	theme	starch/poly	197:207	arg1	blends					255:260	thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends	183:260	thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends	183:260	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	0	27	theme	thermoplastic	29:41	arg1	starch					43:48	thermoplastic starch	29:48	thermoplastic starch	29:48	Morphology and properties of thermoplastic starch blended with biodegradable polyester and filled with halloysite nanoclay.					
32564842	2	28	theme	HNTs	436:439	arg1	contents					441:448	HNTs contents	436:448	HNTs contents	436:448	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	3	29	theme	droplets	629:636	arg1	phases					613:618	dispersed phases	603:618	dispersed phases of small droplets of PBAT or TPS	603:651	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	5	30	theme	blends	1131:1136	arg1	modulus					1071:1077	Young's modulus	1063:1077	Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends	1063:1136	The incorporation of 5 wt% of HNTs improved compatibility and increased Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends approx.					
32564842	2	31	with	nano-biocomposites	372:389	arg1	fractions					422:430	different TPS/PBAT weight fractions	396:430	different TPS/PBAT weight fractions	396:430	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	2	31	with	nano-biocomposites	372:389	arg1	contents					441:448	HNTs contents	436:448	HNTs contents	436:448	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	3	32	theme	homogeneous	716:726	arg1	structure					728:736	a more homogeneous structure	709:736	a more homogeneous structure	709:736	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	4	33	with	biocomposites	780:792	arg1	HNTs					808:811	HNTs	808:811	HNTs	808:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	4	33	with	biocomposites	780:792	arg1	%					803:803	5 wt%	799:803	5 wt% of HNTs	799:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	1	34	theme	clay	156:159	arg1	nanotubes					161:169	halloysite clay nanotubes	145:169	halloysite clay nanotubes (HNTs)	145:176	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	1	34	theme	clay	156:159	arg1	HNTs					172:175	HNTs	172:175	HNTs	172:175	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	4	35	theme	TPS/PBAT/HNTs	766:778	arg1	biocomposites					780:792	the TPS/PBAT/HNTs biocomposites	762:792	the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs	762:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	4	36	theme	wt	801:802	arg1	HNTs					808:811	HNTs	808:811	HNTs	808:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	4	36	theme	wt	801:802	arg1	%					803:803	5 wt%	799:803	5 wt% of HNTs	799:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	4	37	theme	HNTs	808:811	arg1	HNTs					808:811	HNTs	808:811	HNTs	808:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	4	37	theme	HNTs	808:811	arg1	%					803:803	5 wt%	799:803	5 wt% of HNTs	799:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	1	38	theme	halloysite	145:154	arg1	nanotubes					161:169	halloysite clay nanotubes	145:169	halloysite clay nanotubes (HNTs)	145:176	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	1	38	theme	halloysite	145:154	arg1	HNTs					172:175	HNTs	172:175	HNTs	172:175	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32564842	5	39	theme	Young	1063:1067	arg1	modulus					1071:1077	Young's modulus	1063:1077	Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends	1063:1136	The incorporation of 5 wt% of HNTs improved compatibility and increased Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends approx.					
32564842	3	40	theme	TPS	649:651	arg1	droplets					629:636	small droplets	623:636	small droplets of PBAT or TPS	623:651	The TPS80/PBAT20 and TPS20/PBAT80 blends exhibited dispersed phases of small droplets of PBAT or TPS, respectively, whereas the TPS50/PBAT50 blend presented a more homogeneous structure.					
32564842	2	41	theme	different	396:404	arg1	fractions					422:430	different TPS/PBAT weight fractions	396:430	different TPS/PBAT weight fractions	396:430	TPS/PBAT/HNTs nano-biocomposites with different TPS/PBAT weight fractions and HNTs contents were elaborated using a melt blending process, and their morphology and properties were investigated.					
32564842	4	42	from	break	753:757	arg1	Elongation					739:748	Elongation	739:748	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs	739:811	Elongation at break of the TPS/PBAT/HNTs biocomposites with 5 wt% of HNTs significantly increased with increasing PBAT proportion, i.e., 6.5 %, 41.3 %, and 351.5 % for the composites based on TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80, respectively.					
32564842	0	43	theme	biodegradable	63:75	arg1	polyester					77:85	biodegradable polyester	63:85	biodegradable polyester	63:85	Morphology and properties of thermoplastic starch blended with biodegradable polyester and filled with halloysite nanoclay.					
32564842	5	44	theme	wt	1014:1015	arg1	%					1016:1016	5 wt%	1012:1016	5 wt%	1012:1016	The incorporation of 5 wt% of HNTs improved compatibility and increased Young's modulus of the TPS80/PBAT20, TPS50/PBAT50, and TPS20/PBAT80 blends approx.					
32564842	1	45	theme	nanotubes	161:169	arg1	incorporation					128:140	The incorporation	124:140	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends	124:260	The incorporation of halloysite clay nanotubes (HNTs) into thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) blends has been investigated with the aim of improving the compatibility and properties of the matrix.					
32784081	3	0	theme	pH	651:652	arg1	effect					641:646	the effect	637:646	the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium	637:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	3	1	theme	time	663:666	arg1	effect					641:646	the effect	637:646	the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium	637:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	5	2	theme	UT	1080:1081	arg1	models					1083:1088	UT models	1080:1088	UT models	1080:1088	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	5	3	theme	adsorption	1001:1010	arg1	process					1012:1018	the adsorption process	997:1018	the adsorption process	997:1018	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	7	4	theme	copper	1414:1419	arg1	presence					1402:1409	The presence	1398:1409	The presence of copper, nickel and zinc cations	1398:1444	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	8	5	theme	sequential	1578:1587	arg1	treatments					1589:1598	four sequential treatments	1573:1598	four sequential treatments	1573:1598	The adsorbent was reused successfully in four sequential treatments.					
32784081	1	6	theme	fibers	251:256	arg1	surface					240:246	the surface	236:246	the surface of fibers	236:256	In this study, cellulose sulfate was synthesized through sulfonation of cotton, and polypyrrole was coated on the surface of fibers.					
32784081	0	7	theme	sulfate	110:116	arg1	fibers					118:123	cellulose sulfate fibers	100:123	cellulose sulfate fibers	100:123	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	9	8	from	effluent	1743:1750	arg1	percentage					1712:1721	hexavalent chromium removal percentage	1684:1721	hexavalent chromium removal percentage from electroplating effluent (99.87%)	1684:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	9	8	from	effluent	1743:1750	arg1	efficiency					1670:1679	the high efficiency	1661:1679	the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%)	1661:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	4	9	theme	optimum	817:823	arg1	conditions					825:834	the optimum conditions	813:834	the optimum conditions	813:834	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	5	10	theme	Langmuir	1145:1152	arg1	model					1154:1158	the Langmuir model	1141:1158	the Langmuir model	1141:1158	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	3	11	theme	adsorption	754:763	arg1	process					765:771	the adsorption process	750:771	the adsorption process	750:771	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	0	12	theme	cellulose	100:108	arg1	fibers					118:123	cellulose sulfate fibers	100:123	cellulose sulfate fibers	100:123	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	0	13	from	wastewater	61:70	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole	0:88	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	2	14	theme	optimum	269:275	arg1	ratio					277:281	the optimum ratio	265:281	the optimum ratio of pyrrole to cellulose sulfate	265:313	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	7	15	theme	zinc	1433:1436	arg1	presence					1402:1409	The presence	1398:1409	The presence of copper, nickel and zinc cations	1398:1444	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	2	16	theme	TGA	454:456	arg1	analysis					458:465	TGA analysis	454:465	TGA analysis	454:465	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	5	17	theme	adsorption	959:968	arg1	isotherms					970:978	adsorption isotherms	959:978	adsorption isotherms	959:978	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	9	18	theme	high	1665:1668	arg1	efficiency					1670:1679	the high efficiency	1661:1679	the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%)	1661:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	4	19	theme	contact	920:926	arg1	time					928:931	contact time	920:931	contact time	920:931	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	7	20	theme	hexavalent	1497:1506	arg1	chromium					1508:1515	hexavalent chromium	1497:1515	hexavalent chromium	1497:1515	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	9	21	theme	removal	1704:1710	arg1	percentage					1712:1721	hexavalent chromium removal percentage	1684:1721	hexavalent chromium removal percentage from electroplating effluent (99.87%)	1684:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	2	22	theme	morphological	362:374	arg1	properties					376:385	the physical, chemical, and morphological properties	334:385	the physical, chemical, and morphological properties of the composite	334:402	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	4	23	dep	%	805:805	arg1	dose					847:850	adsorbent dose	837:850	adsorbent dose	837:850	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	5	24	theme	isotherms	970:978	arg1	results					948:954	The results	944:954	The results of adsorption isotherms	944:978	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	4	25	theme	removal	778:784	arg1	percentage					786:795	The removal percentage	774:795	The removal percentage	774:795	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	25	theme	removal	778:784	arg1	%					805:805	99.9%	801:805	99.9%	801:805	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	6	26	theme	spontaneous	1321:1331	arg1	adsorption					1333:1342	the endothermic and spontaneous adsorption	1301:1342	the endothermic and spontaneous adsorption	1301:1342	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	4	27	theme	Cr	887:888	arg1	concentration					870:882	initial concentration	862:882	initial concentration of Cr(VI)	862:892	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	9	28	theme	chromium	1695:1702	arg1	percentage					1712:1721	hexavalent chromium removal percentage	1684:1721	hexavalent chromium removal percentage from electroplating effluent (99.87%)	1684:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	3	29	theme	contact	655:661	arg1	time					663:666	contact time	655:666	contact time	655:666	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	30	theme	diffusion	1274:1282	arg1	model					1284:1288	the intraparticle diffusion model	1256:1288	the intraparticle diffusion model	1256:1288	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	2	31	theme	cellulose	297:305	arg1	sulfate					307:313	cellulose sulfate	297:313	cellulose sulfate	297:313	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	0	32	theme	Efficient	0:8	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole	0:88	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	7	33	dep	copper	1414:1419	arg1	cations					1438:1444	cations	1438:1444	cations	1438:1444	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	6	34	from	surface	1389:1395	arg1	increase					1352:1359	an increase	1349:1359	an increase in entropy on the adsorbent surface	1349:1395	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	9	35	from	efficiency	1670:1679	arg1	effluent					1743:1750	electroplating effluent	1728:1750	electroplating effluent (99.87%)	1728:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	9	35	from	efficiency	1670:1679	arg1	%					1758:1758	99.87%	1753:1758	99.87%	1753:1758	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	2	36	theme	chemical	348:355	arg1	properties					376:385	the physical, chemical, and morphological properties	334:385	the physical, chemical, and morphological properties of the composite	334:402	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	0	37	theme	hexavalent	21:30	arg1	chromium					32:39	hexavalent chromium	21:39	hexavalent chromium	21:39	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	4	38	dep	Cr	887:888	arg1	VI					890:891	VI	890:891	VI	890:891	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	9	39	theme	percentage	1712:1721	arg1	efficiency					1670:1679	the high efficiency	1661:1679	the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%)	1661:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	2	40	theme	pyrrole	286:292	arg1	ratio					277:281	the optimum ratio	265:281	the optimum ratio of pyrrole to cellulose sulfate	265:313	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	3	41	with	experiments	584:594	arg1	technique					617:625	the Box-Behnken technique	601:625	the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process	601:771	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	2	42	theme	composite	394:402	arg1	properties					376:385	the physical, chemical, and morphological properties	334:385	the physical, chemical, and morphological properties of the composite	334:402	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	6	43	theme	endothermic	1305:1315	arg1	adsorption					1333:1342	the endothermic and spontaneous adsorption	1301:1342	the endothermic and spontaneous adsorption	1301:1342	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	3	44	theme	adsorbent	669:677	arg1	dose					679:682	adsorbent dose	669:682	adsorbent dose	669:682	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	45	theme	thermodynamic	1201:1213	arg1	studies					1215:1221	the kinetic and thermodynamic studies	1185:1221	the kinetic and thermodynamic studies	1185:1221	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	3	46	theme	chromium	506:513	arg1	adsorption					481:490	adsorption	481:490	adsorption of hexavalent chromium using the composite adsorbent	481:543	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	47	theme	adsorption	1228:1237	arg1	process					1239:1245	the adsorption process	1224:1245	the adsorption process	1224:1245	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	6	48	from	increase	1352:1359	arg1	surface					1389:1395	the adsorbent surface	1375:1395	the adsorbent surface	1375:1395	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	6	48	from	increase	1352:1359	arg1	entropy					1364:1370	entropy	1364:1370	entropy on the adsorbent surface	1364:1395	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	9	49	theme	electroplating	1728:1741	arg1	effluent					1743:1750	electroplating effluent	1728:1750	electroplating effluent (99.87%)	1728:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	9	49	theme	electroplating	1728:1741	arg1	%					1758:1758	99.87%	1753:1758	99.87%	1753:1758	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	3	50	theme	dose	679:682	arg1	effect					641:646	the effect	637:646	the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium	637:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	7	51	theme	nickel	1422:1427	arg1	presence					1402:1409	The presence	1398:1409	The presence of copper, nickel and zinc cations	1398:1444	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	1	52	theme	cotton	198:203	arg1	sulfonation					183:193	sulfonation	183:193	sulfonation of cotton	183:203	In this study, cellulose sulfate was synthesized through sulfonation of cotton, and polypyrrole was coated on the surface of fibers.					
32784081	0	53	theme	chromium	32:39	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole	0:88	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	4	54	theme	200 mg	895:900	arg1	L-1					902:904	200 mg L-1	895:904	200 mg L-1	895:904	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	3	55	theme	designed	575:582	arg1	experiments					584:594	designed experiments	575:594	designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process	575:771	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	5	56	theme	maximum	1110:1116	arg1	capacity					1129:1136	the calculated maximum adsorption capacity	1095:1136	the calculated maximum adsorption capacity by the Langmuir model	1095:1158	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	5	56	theme	maximum	1110:1116	arg1	g-1					1171:1173	198 mg g-1	1164:1173	198 mg g-1	1164:1173	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	7	57	theme	removal	1475:1481	arg1	percentage					1483:1492	the removal percentage	1471:1492	the removal percentage of hexavalent chromium	1471:1515	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	4	58	theme	pH	907:908	arg1	value					910:914	pH value	907:914	pH value	907:914	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	3	59	theme	hexavalent	717:726	arg1	chromium					728:735	hexavalent chromium	717:735	hexavalent chromium	717:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	3	60	theme	experiments	584:594	arg1	results					564:570	the results	560:570	the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process	560:771	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	7	61	contain	had	1446:1448	arg2	effect					1461:1466	no adverse effect	1450:1466	no adverse effect	1450:1466	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	7	61	contain	had	1446:1448	arg1	presence					1402:1409	The presence	1398:1409	The presence of copper, nickel and zinc cations	1398:1444	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	0	62	theme	electroplating	46:59	arg1	wastewater					61:70	electroplating wastewater	46:70	electroplating wastewater using polypyrrole	46:88	Efficient removal of hexavalent chromium from electroplating wastewater using polypyrrole coated on cellulose sulfate fibers.					
32784081	3	63	theme	initial	692:698	arg1	concentration					700:712	the initial concentration	688:712	the initial concentration of hexavalent chromium	688:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	64	theme	adsorbent	1379:1387	arg1	surface					1389:1395	the adsorbent surface	1375:1395	the adsorbent surface	1375:1395	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	3	65	theme	concentration	700:712	arg1	effect					641:646	the effect	637:646	the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium	637:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	66	from	entropy	1364:1370	arg1	surface					1389:1395	the adsorbent surface	1375:1395	the adsorbent surface	1375:1395	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	3	67	theme	hexavalent	495:504	arg1	chromium					506:513	hexavalent chromium	495:513	hexavalent chromium using the composite adsorbent	495:543	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	68	theme	kinetic	1189:1195	arg1	studies					1215:1221	the kinetic and thermodynamic studies	1185:1221	the kinetic and thermodynamic studies	1185:1221	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	4	69	theme	adsorbent	837:845	arg1	dose					847:850	adsorbent dose	837:850	adsorbent dose	837:850	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	3	70	theme	Box-Behnken	605:615	arg1	technique					617:625	the Box-Behnken technique	601:625	the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process	601:771	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	9	71	theme	hexavalent	1684:1693	arg1	percentage					1712:1721	hexavalent chromium removal percentage	1684:1721	hexavalent chromium removal percentage from electroplating effluent (99.87%)	1684:1759	Consequently, the synthesized adsorbent is efficient due to the high efficiency of hexavalent chromium removal percentage from electroplating effluent (99.87%).					
32784081	7	72	theme	chromium	1508:1515	arg1	percentage					1483:1492	the removal percentage	1471:1492	the removal percentage of hexavalent chromium	1471:1515	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	5	73	theme	198 mg	1164:1169	arg1	capacity					1129:1136	the calculated maximum adsorption capacity	1095:1136	the calculated maximum adsorption capacity by the Langmuir model	1095:1158	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	5	73	theme	198 mg	1164:1169	arg1	g-1					1171:1173	198 mg g-1	1164:1173	198 mg g-1	1164:1173	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	5	74	theme	calculated	1099:1108	arg1	capacity					1129:1136	the calculated maximum adsorption capacity	1095:1136	the calculated maximum adsorption capacity by the Langmuir model	1095:1158	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	5	74	theme	calculated	1099:1108	arg1	g-1					1171:1173	198 mg g-1	1164:1173	198 mg g-1	1164:1173	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	4	75	dep	dose	847:850	arg1	L-1					857:859	4 g L-1	853:859	4 g L-1	853:859	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	75	dep	dose	847:850	arg1	time					928:931	contact time	920:931	contact time	920:931	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	75	dep	dose	847:850	arg1	200 min					934:940	200 min	934:940	200 min	934:940	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	75	dep	dose	847:850	arg1	value					910:914	pH value	907:914	pH value	907:914	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	75	dep	dose	847:850	arg1	2					917:917	2	917:917	2	917:917	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	75	dep	dose	847:850	arg1	concentration					870:882	initial concentration	862:882	initial concentration of Cr(VI)	862:892	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	4	75	dep	dose	847:850	arg1	L-1					902:904	200 mg L-1	895:904	200 mg L-1	895:904	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
32784081	7	76	theme	adverse	1453:1459	arg1	effect					1461:1466	no adverse effect	1450:1466	no adverse effect	1450:1466	The presence of copper, nickel and zinc cations had no adverse effect on the removal percentage of hexavalent chromium significantly.					
32784081	1	77	theme	cellulose	141:149	arg1	sulfate					151:157	cellulose sulfate	141:157	cellulose sulfate	141:157	In this study, cellulose sulfate was synthesized through sulfonation of cotton, and polypyrrole was coated on the surface of fibers.					
32784081	3	78	theme	chromium	728:735	arg1	concentration					700:712	the initial concentration	688:712	the initial concentration of hexavalent chromium	688:735	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	3	78	theme	chromium	728:735	arg1	pH					651:652	pH	651:652	pH	651:652	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	3	78	theme	chromium	728:735	arg1	time					663:666	contact time	655:666	contact time	655:666	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	3	78	theme	chromium	728:735	arg1	dose					679:682	adsorbent dose	669:682	adsorbent dose	669:682	Furthermore, adsorption of hexavalent chromium using the composite adsorbent was studied by the results of designed experiments with the Box-Behnken technique to assess the effect of pH, contact time, adsorbent dose and the initial concentration of hexavalent chromium and optimize the adsorption process.					
32784081	6	79	theme	intraparticle	1260:1272	arg1	model					1284:1288	the intraparticle diffusion model	1256:1288	the intraparticle diffusion model	1256:1288	Based on the kinetic and thermodynamic studies, the adsorption process followed the intraparticle diffusion model and showed the endothermic and spontaneous adsorption with an increase in entropy on the adsorbent surface.					
32784081	5	80	theme	adsorption	1118:1127	arg1	capacity					1129:1136	the calculated maximum adsorption capacity	1095:1136	the calculated maximum adsorption capacity by the Langmuir model	1095:1158	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	5	80	theme	adsorption	1118:1127	arg1	g-1					1171:1173	198 mg g-1	1164:1173	198 mg g-1	1164:1173	The results of adsorption isotherms illustrated that the adsorption process followed Redlich-Peterson, Freundlich, Radke-Prausnitz, and UT models, and the calculated maximum adsorption capacity by the Langmuir model was 198 mg g-1.					
32784081	2	81	theme	physical	338:345	arg1	properties					376:385	the physical, chemical, and morphological properties	334:385	the physical, chemical, and morphological properties of the composite	334:402	Then, the optimum ratio of pyrrole to cellulose sulfate was evaluated, and the physical, chemical, and morphological properties of the composite were assessed by using FESEM, EDS, FTIR, BET, and TGA analysis.					
32784081	4	82	theme	initial	862:868	arg1	concentration					870:882	initial concentration	862:882	initial concentration of Cr(VI)	862:892	The removal percentage was 99.9% under the optimum conditions (adsorbent dose, 4 g L-1; initial concentration of Cr(VI), 200 mg L-1; pH value, 2; contact time, 200 min).					
33049534	11	0	theme	soil	2155:2158	arg1	aggregates					2160:2169	soil aggregates	2155:2169	soil aggregates	2155:2169	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	11	1	theme	equation	2122:2129	arg1	modelling					2131:2139	pH. Structural equation modelling	2107:2139	pH. Structural equation modelling	2107:2139	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	4	2	theme	organic	604:610	arg1	matter					612:617	organic matter	604:617	organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm)	604:731	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	10	3	theme	Eurotiomycetes	1892:1905	arg1	abundance					1842:1850	The relative abundance	1829:1850	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes	1829:1905	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes revealed significant differences among the aggregates.					
33049534	7	4	theme	DOM	1384:1386	arg1	complexity					1370:1379	the structural complexity	1355:1379	the structural complexity of DOM	1355:1386	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	8	5	theme	genes	1587:1591	arg1	diversity					1542:1550	diversity	1542:1550	diversity	1542:1550	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	8	5	theme	genes	1587:1591	arg1	abundance					1528:1536	abundance	1528:1536	abundance	1528:1536	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	3	6	from	roles	526:530	arg1	mineralization					550:563	organic matter mineralization	535:563	organic matter mineralization	535:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	2	7	theme	microbial	293:301	arg1	matter					282:287	Labile organic matter	267:287	Labile organic matter	267:287	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	7	theme	microbial	293:301	arg1	community					303:311	microbial community	293:311	microbial community	293:311	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	7	theme	microbial	293:301	arg1	factors					333:339	vital intrinsic factors	317:339	vital intrinsic factors	317:339	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	11	8	theme	matter	2338:2343	arg1	mineralization					2345:2358	organic matter mineralization	2330:2358	organic matter mineralization	2330:2358	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	6	9	theme	sequence	1208:1215	arg1	Ck < NPK < AM					1217:1229	the sequence Ck < NPK < AM	1204:1229	the sequence Ck < NPK < AM	1204:1229	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	3	10	theme	matter	543:548	arg1	mineralization					550:563	organic matter mineralization	535:563	organic matter mineralization	535:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	4	11	from	macroaggregates	664:678	arg1	mineralization					586:599	The mineralization	582:599	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM)	582:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	7	12	theme	opposite	1401:1408	arg1	trend					1410:1414	the opposite trend	1397:1414	the opposite trend	1397:1414	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	6	13	theme	cumulative	1095:1104	arg1	emissions					1112:1120	The cumulative CO2-C emissions	1091:1120	The cumulative CO2-C emissions	1091:1120	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	5	14	dep	abundance	947:955	arg1	the					943:945	the	943:945	the	943:945	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	3	15	from	community	505:513	arg1	mineralization					550:563	organic matter mineralization	535:563	organic matter mineralization	535:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	2	16	theme	intrinsic	323:331	arg1	matter					282:287	Labile organic matter	267:287	Labile organic matter	267:287	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	16	theme	intrinsic	323:331	arg1	community					303:311	microbial community	293:311	microbial community	293:311	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	16	theme	intrinsic	323:331	arg1	factors					333:339	vital intrinsic factors	317:339	vital intrinsic factors	317:339	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	9	17	theme	microbial	1639:1647	arg1	Alphaproteobacteria					1735:1753	Alphaproteobacteria	1735:1753	Alphaproteobacteria	1735:1753	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	9	17	theme	microbial	1639:1647	arg1	classes					1649:1655	The dominant microbial classes	1626:1655	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates	1626:1712	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	5	18	theme	GH1	998:1000	arg1	family					1023:1028	glycoside hydrolase family 1	1003:1030	glycoside hydrolase family 1	1003:1030	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	18	theme	GH1	998:1000	arg1	genes					1066:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	1	19	theme	organic	214:220	arg1	matter					222:227	soil organic matter	209:227	soil organic matter	209:227	The mineralization of soil organic matter is closely related to climate change.					
33049534	5	20	theme	glycoside	1003:1011	arg1	family					1023:1028	glycoside hydrolase family 1	1003:1030	glycoside hydrolase family 1	1003:1030	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	20	theme	glycoside	1003:1011	arg1	genes					1066:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	9	21	theme	β-glucosidase	1671:1683	arg1	genes					1685:1689	GH1 β-glucosidase genes	1667:1689	GH1 β-glucosidase genes	1667:1689	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	6	22	theme	fertilization	1167:1179	arg1	treatment					1181:1189	each fertilization treatment	1162:1189	each fertilization treatment	1162:1189	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	11	23	theme	Redundancy	1962:1971	arg1	analysis					1973:1980	Redundancy analysis	1962:1980	Redundancy analysis	1962:1980	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	11	24	theme	pH.	2107:2109	arg1	modelling					2131:2139	pH. Structural equation modelling	2107:2139	pH. Structural equation modelling	2107:2139	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	11	25	theme	GH1	2007:2009	arg1	community					2025:2033	microbial GH1 β-glucosidase community	1997:2033	microbial GH1 β-glucosidase community in the soil aggregates	1997:2056	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	6	26	theme	aggregate	1247:1255	arg1	size					1239:1242	each size	1234:1242	each size of aggregate	1234:1255	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	4	27	dep	macroaggregates	628:642	arg1	>2 mm					650:654	>2 mm	650:654	>2 mm	650:654	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	27	dep	macroaggregates	628:642	arg1	LMA					645:647	LMA	645:647	LMA	645:647	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	2	28	theme	Labile	267:272	arg1	matter					282:287	Labile organic matter	267:287	Labile organic matter	267:287	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	28	theme	Labile	267:272	arg1	community					303:311	microbial community	293:311	microbial community	293:311	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	28	theme	Labile	267:272	arg1	factors					333:339	vital intrinsic factors	317:339	vital intrinsic factors	317:339	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	9	29	theme	soil	1698:1701	arg1	aggregates					1703:1712	the soil aggregates	1694:1712	the soil aggregates	1694:1712	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	6	30	theme	order	1138:1142	arg1	LMA < SMA < MI					1144:1157	the order LMA < SMA < MI	1134:1157	the order LMA < SMA < MI in each fertilization treatment	1134:1189	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	5	31	theme	β-glucosidase	1043:1055	arg1	family					1023:1028	glycoside hydrolase family 1	1003:1030	glycoside hydrolase family 1	1003:1030	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	31	theme	β-glucosidase	1043:1055	arg1	genes					1066:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	11	32	theme	microbial	1997:2005	arg1	community					2025:2033	microbial GH1 β-glucosidase community	1997:2033	microbial GH1 β-glucosidase community in the soil aggregates	1997:2056	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	0	33	theme	matter	164:169	arg1	mineralization					171:184	organic matter mineralization	156:184	organic matter mineralization	156:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	5	34	dep	concentration	876:888	arg1	The					872:874	The	872:874	The	872:874	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	0	35	theme	organic	78:84	arg1	matter					86:91	dissolved organic matter	68:91	dissolved organic matter	68:91	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	5	36	theme	genes	1066:1070	arg1	composition					983:993	community composition	973:993	community composition	973:993	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	36	theme	genes	1066:1070	arg1	activity					912:919	activity	912:919	activity of β-glucosidase	912:936	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	36	theme	genes	1066:1070	arg1	abundance					947:955	abundance	947:955	abundance	947:955	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	36	theme	genes	1066:1070	arg1	concentration					876:888	concentration	876:888	concentration	876:888	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	36	theme	genes	1066:1070	arg1	diversity					958:966	diversity	958:966	diversity	958:966	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	36	theme	genes	1066:1070	arg1	structure					894:902	structure	894:902	structure	894:902	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	11	37	theme	β-glucosidase	2295:2307	arg1	genes					2309:2313	the GH1 microbial β-glucosidase genes	2277:2313	the GH1 microbial β-glucosidase genes	2277:2313	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	8	38	theme	smaller	1454:1460	arg1	aggregates					1462:1471	the smaller aggregates	1450:1471	the smaller aggregates	1450:1471	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	6	39	from	LMA < SMA < MI	1144:1157	arg1	treatment					1181:1189	each fertilization treatment	1162:1189	each fertilization treatment	1162:1189	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	4	40	from	matter	612:617	arg1	macroaggregates					664:678	small macroaggregates	658:678	small macroaggregates (SMA, 0.25-2 mm)	658:695	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	40	from	matter	612:617	arg1	microaggregates					702:716	microaggregates	702:716	microaggregates (MI, <0.25 mm)	702:731	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	40	from	matter	612:617	arg1	macroaggregates					628:642	large macroaggregates	622:642	large macroaggregates (LMA, >2 mm)	622:655	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	11	41	theme	soil	2042:2045	arg1	aggregates					2047:2056	the soil aggregates	2038:2056	the soil aggregates	2038:2056	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	4	42	from	Ultisol	741:747	arg1	mineralization					586:599	The mineralization	582:599	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM)	582:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	11	43	theme	GH1	2281:2283	arg1	genes					2309:2313	the GH1 microbial β-glucosidase genes	2277:2313	the GH1 microbial β-glucosidase genes	2277:2313	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	0	44	theme	hydrolyzing	105:115	arg1	community					127:135	cellobiose hydrolyzing microbial community	94:135	cellobiose hydrolyzing microbial community	94:135	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	11	45	theme	DOM	2219:2221	arg1	concentration					2223:2235	DOM concentration	2219:2235	DOM concentration	2219:2235	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	3	46	theme	organic	446:452	arg1	DOM					462:464	DOM	462:464	DOM	462:464	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	3	46	theme	organic	446:452	arg1	matter					454:459	dissolved organic matter	436:459	dissolved organic matter (DOM)	436:465	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	7	47	theme	DOM	1279:1281	arg1	concentration					1262:1274	The concentration	1258:1274	The concentration of DOM in the soil aggregates	1258:1304	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	7	48	theme	soil	1290:1293	arg1	aggregates					1295:1304	the soil aggregates	1286:1304	the soil aggregates	1286:1304	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	11	49	theme	DOM	2085:2087	arg1	concentration					2089:2101	DOM concentration	2085:2101	DOM concentration	2085:2101	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	0	50	theme	soil	14:17	arg1	size					29:32	soil aggregate size	14:32	soil aggregate size	14:32	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	8	51	theme	larger	1501:1506	arg1	aggregates					1508:1517	the larger aggregates	1497:1517	the larger aggregates	1497:1517	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	0	52	theme	size	29:32	arg1	Regulation					0:9	Regulation	0:9	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.	0:185	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	3	53	theme	aggregate	418:426	arg1	size					428:431	soil aggregate size	413:431	soil aggregate size	413:431	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	0	54	theme	different	40:48	arg1	fertilizations					50:63	different fertilizations	40:63	different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization	40:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	10	55	theme	Sordariomycetes	1871:1885	arg1	abundance					1842:1850	The relative abundance	1829:1850	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes	1829:1905	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes revealed significant differences among the aggregates.					
33049534	3	56	theme	hydrolyzing	483:493	arg1	community					505:513	the cellobiose hydrolyzing microbial community	468:513	the cellobiose hydrolyzing microbial community	468:513	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	4	57	theme	large	622:626	arg1	macroaggregates					628:642	large macroaggregates	622:642	large macroaggregates (LMA, >2 mm)	622:655	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	10	58	theme	Actinobacteria	1855:1868	arg1	abundance					1842:1850	The relative abundance	1829:1850	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes	1829:1905	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes revealed significant differences among the aggregates.					
33049534	3	59	from	matter	454:459	arg1	mineralization					550:563	organic matter mineralization	535:563	organic matter mineralization	535:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	2	60	theme	matter	391:396	arg1	mineralization					360:373	the mineralization	356:373	the mineralization of soil organic matter	356:396	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	11	61	from	community	2025:2033	arg1	aggregates					2047:2056	the soil aggregates	2038:2056	the soil aggregates	2038:2056	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	0	62	from	community	127:135	arg1	mineralization					171:184	organic matter mineralization	156:184	organic matter mineralization	156:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	2	63	theme	soil	378:381	arg1	matter					391:396	soil organic matter	378:396	soil organic matter	378:396	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	4	64	theme	animal	825:830	arg1	manure					832:837	animal manure	825:837	animal manure (AM)	825:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	64	theme	animal	825:830	arg1	AM					840:841	AM	840:841	AM	840:841	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	8	65	theme	GH1	1559:1561	arg1	genes					1587:1591	the GH1 microbial β-glucosidase genes	1555:1591	the GH1 microbial β-glucosidase genes	1555:1591	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	8	66	theme	β-glucosidase	1573:1585	arg1	genes					1587:1591	the GH1 microbial β-glucosidase genes	1555:1591	the GH1 microbial β-glucosidase genes	1555:1591	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	11	67	theme	organic	2330:2336	arg1	mineralization					2345:2358	organic matter mineralization	2330:2358	organic matter mineralization	2330:2358	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	4	68	theme	matter	612:617	arg1	mineralization					586:599	The mineralization	582:599	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM)	582:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	3	69	theme	organic	535:541	arg1	mineralization					550:563	organic matter mineralization	535:563	organic matter mineralization	535:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	11	70	theme	β-glucosidase	2192:2204	arg1	activity					2206:2213	the β-glucosidase activity	2188:2213	the β-glucosidase activity	2188:2213	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	6	71	theme	CO2-C	1106:1110	arg1	emissions					1112:1120	The cumulative CO2-C emissions	1091:1120	The cumulative CO2-C emissions	1091:1120	The cumulative CO2-C emissions occurred in the order LMA < SMA < MI in each fertilization treatment and followed the sequence Ck < NPK < AM in each size of aggregate.					
33049534	4	72	theme	small	658:662	arg1	macroaggregates					664:678	small macroaggregates	658:678	small macroaggregates (SMA, 0.25-2 mm)	658:695	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	9	73	theme	dominant	1630:1637	arg1	Alphaproteobacteria					1735:1753	Alphaproteobacteria	1735:1753	Alphaproteobacteria	1735:1753	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	9	73	theme	dominant	1630:1637	arg1	classes					1649:1655	The dominant microbial classes	1626:1655	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates	1626:1712	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	7	74	theme	structural	1359:1368	arg1	complexity					1370:1379	the structural complexity	1355:1379	the structural complexity of DOM	1355:1386	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	2	75	theme	vital	317:321	arg1	matter					282:287	Labile organic matter	267:287	Labile organic matter	267:287	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	75	theme	vital	317:321	arg1	community					303:311	microbial community	293:311	microbial community	293:311	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	75	theme	vital	317:321	arg1	factors					333:339	vital intrinsic factors	317:339	vital intrinsic factors	317:339	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	1	76	theme	soil	209:212	arg1	matter					222:227	soil organic matter	209:227	soil organic matter	209:227	The mineralization of soil organic matter is closely related to climate change.					
33049534	0	77	from	matter	86:91	arg1	mineralization					171:184	organic matter mineralization	156:184	organic matter mineralization	156:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	4	78	located	observed	848:855	arg2	mineralization					586:599	The mineralization	582:599	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM)	582:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	78	located	observed	848:855	arg1	study					865:869	this study	860:869	this study	860:869	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	8	79	theme	microbial	1563:1571	arg1	genes					1587:1591	the GH1 microbial β-glucosidase genes	1555:1591	the GH1 microbial β-glucosidase genes	1555:1591	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	1	80	theme	matter	222:227	arg1	mineralization					191:204	The mineralization	187:204	The mineralization of soil organic matter	187:227	The mineralization of soil organic matter is closely related to climate change.					
33049534	1	80	theme	matter	222:227	arg1	related					240:246	related	240:246	related	240:246	The mineralization of soil organic matter is closely related to climate change.					
33049534	9	81	theme	GH1	1667:1669	arg1	genes					1685:1689	GH1 β-glucosidase genes	1667:1689	GH1 β-glucosidase genes	1667:1689	The dominant microbial classes harboring GH1 β-glucosidase genes in the soil aggregates were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Flavobacteria, Eurotiomycetes, and Sordariomycetes.					
33049534	0	82	theme	organic	156:162	arg1	mineralization					171:184	organic matter mineralization	156:184	organic matter mineralization	156:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	5	83	theme	hydrolase	1013:1021	arg1	family					1023:1028	glycoside hydrolase family 1	1003:1030	glycoside hydrolase family 1	1003:1030	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	83	theme	hydrolase	1013:1021	arg1	genes					1066:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	7	84	theme	aggregate	1323:1331	arg1	size					1333:1336	the aggregate size	1319:1336	the aggregate size	1319:1336	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	5	85	theme	community	973:981	arg1	composition					983:993	community composition	973:993	community composition	973:993	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	2	86	theme	organic	274:280	arg1	matter					282:287	Labile organic matter	267:287	Labile organic matter	267:287	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	86	theme	organic	274:280	arg1	community					303:311	microbial community	293:311	microbial community	293:311	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	2	86	theme	organic	274:280	arg1	factors					333:339	vital intrinsic factors	317:339	vital intrinsic factors	317:339	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	11	87	theme	Structural	2111:2120	arg1	modelling					2131:2139	pH. Structural equation modelling	2107:2139	pH. Structural equation modelling	2107:2139	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	8	88	theme	β-glucosidase	1433:1445	arg1	activity					1421:1428	The activity	1417:1428	The activity of β-glucosidase in the smaller aggregates	1417:1471	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	8	88	theme	β-glucosidase	1433:1445	arg1	higher					1477:1482	higher	1477:1482	higher	1477:1482	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	0	89	theme	dissolved	68:76	arg1	matter					86:91	dissolved organic matter	68:91	dissolved organic matter	68:91	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	11	90	theme	β-glucosidase	2011:2023	arg1	community					2025:2033	microbial GH1 β-glucosidase community	1997:2033	microbial GH1 β-glucosidase community in the soil aggregates	1997:2056	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	4	91	from	macroaggregates	628:642	arg1	mineralization					586:599	The mineralization	582:599	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM)	582:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	5	92	theme	encoding	1057:1064	arg1	family					1023:1028	glycoside hydrolase family 1	1003:1030	glycoside hydrolase family 1	1003:1030	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	92	theme	encoding	1057:1064	arg1	genes					1066:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	8	93	dep	abundance	1528:1536	arg1	the					1524:1526	the	1524:1526	the	1524:1526	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	0	94	theme	cellobiose	94:103	arg1	community					127:135	cellobiose hydrolyzing microbial community	94:135	cellobiose hydrolyzing microbial community	94:135	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	5	95	theme	DOM	907:909	arg1	composition					983:993	community composition	973:993	community composition	973:993	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	95	theme	DOM	907:909	arg1	activity					912:919	activity	912:919	activity of β-glucosidase	912:936	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	95	theme	DOM	907:909	arg1	abundance					947:955	abundance	947:955	abundance	947:955	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	95	theme	DOM	907:909	arg1	concentration					876:888	concentration	876:888	concentration	876:888	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	95	theme	DOM	907:909	arg1	diversity					958:966	diversity	958:966	diversity	958:966	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	95	theme	DOM	907:909	arg1	structure					894:902	structure	894:902	structure	894:902	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	11	96	theme	genes	2309:2313	arg1	activity					2206:2213	the β-glucosidase activity	2188:2213	the β-glucosidase activity	2188:2213	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	11	96	theme	genes	2309:2313	arg1	concentration					2223:2235	DOM concentration	2219:2235	DOM concentration	2219:2235	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	11	96	theme	genes	2309:2313	arg1	diversity					2264:2272	diversity	2264:2272	diversity	2264:2272	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	11	96	theme	genes	2309:2313	arg1	abundance					2250:2258	abundance	2250:2258	abundance	2250:2258	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	0	97	from	fertilizations	50:63	arg1	community					127:135	cellobiose hydrolyzing microbial community	94:135	cellobiose hydrolyzing microbial community	94:135	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	0	97	from	fertilizations	50:63	arg1	roles					147:151	their roles	141:151	their roles in organic matter mineralization	141:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	0	97	from	fertilizations	50:63	arg1	matter					86:91	dissolved organic matter	68:91	dissolved organic matter	68:91	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	0	98	theme	microbial	117:125	arg1	community					127:135	cellobiose hydrolyzing microbial community	94:135	cellobiose hydrolyzing microbial community	94:135	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	3	99	from	Regulation	399:408	arg1	community					505:513	the cellobiose hydrolyzing microbial community	468:513	the cellobiose hydrolyzing microbial community	468:513	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	3	99	from	Regulation	399:408	arg1	DOM					462:464	DOM	462:464	DOM	462:464	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	3	99	from	Regulation	399:408	arg1	roles					526:530	their roles	520:530	their roles in organic matter mineralization	520:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	3	99	from	Regulation	399:408	arg1	matter					454:459	dissolved organic matter	436:459	dissolved organic matter (DOM)	436:465	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	8	100	theme	same	1614:1617	arg1	trend					1619:1623	the same trend	1610:1623	the same trend	1610:1623	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	11	101	theme	microbial	2285:2293	arg1	genes					2309:2313	the GH1 microbial β-glucosidase genes	2277:2313	the GH1 microbial β-glucosidase genes	2277:2313	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	8	102	from	activity	1421:1428	arg1	aggregates					1462:1471	the smaller aggregates	1450:1471	the smaller aggregates	1450:1471	The activity of β-glucosidase in the smaller aggregates was higher than that in the larger aggregates, and the abundance and diversity of the GH1 microbial β-glucosidase genes generally echoed the same trend.					
33049534	4	103	from	mineralization	586:599	arg1	macroaggregates					664:678	small macroaggregates	658:678	small macroaggregates (SMA, 0.25-2 mm)	658:695	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	103	from	mineralization	586:599	arg1	microaggregates					702:716	microaggregates	702:716	microaggregates (MI, <0.25 mm)	702:731	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	103	from	mineralization	586:599	arg1	macroaggregates					628:642	large macroaggregates	622:642	large macroaggregates (LMA, >2 mm)	622:655	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	5	104	theme	microbial	1033:1041	arg1	family					1023:1028	glycoside hydrolase family 1	1003:1030	glycoside hydrolase family 1	1003:1030	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	104	theme	microbial	1033:1041	arg1	genes					1066:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes	998:1070	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	3	105	theme	soil	413:416	arg1	size					428:431	soil aggregate size	413:431	soil aggregate size	413:431	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	11	106	dep	abundance	2250:2258	arg1	the					2246:2248	the	2246:2248	the	2246:2248	Redundancy analysis confirmed that microbial GH1 β-glucosidase community in the soil aggregates was primarily regulated by DOM concentration and pH. Structural equation modelling revealed that soil aggregates mainly regulated the β-glucosidase activity and DOM concentration and then the abundance and diversity of the GH1 microbial β-glucosidase genes in controlling organic matter mineralization.					
33049534	0	107	theme	aggregate	19:27	arg1	size					29:32	soil aggregate size	14:32	soil aggregate size	14:32	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	4	108	dep	microaggregates	702:716	arg1	<0.25 mm					723:730	<0.25 mm	723:730	<0.25 mm	723:730	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	108	dep	microaggregates	702:716	arg1	MI					719:720	MI	719:720	MI	719:720	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	3	109	theme	cellobiose	472:481	arg1	community					505:513	the cellobiose hydrolyzing microbial community	468:513	the cellobiose hydrolyzing microbial community	468:513	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	3	110	theme	dissolved	436:444	arg1	DOM					462:464	DOM	462:464	DOM	462:464	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	3	110	theme	dissolved	436:444	arg1	matter					454:459	dissolved organic matter	436:459	dissolved organic matter (DOM)	436:465	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	10	111	theme	relative	1833:1840	arg1	abundance					1842:1850	The relative abundance	1829:1850	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes	1829:1905	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes revealed significant differences among the aggregates.					
33049534	3	112	theme	size	428:431	arg1	Regulation					399:408	Regulation	399:408	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization	399:563	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	4	113	theme	chemical	794:801	arg1	NPK					816:818	NPK	816:818	NPK	816:818	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	113	theme	chemical	794:801	arg1	fertilizers					803:813	chemical fertilizers	794:813	chemical fertilizers (NPK)	794:819	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	5	114	theme	β-glucosidase	924:936	arg1	composition					983:993	community composition	973:993	community composition	973:993	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	114	theme	β-glucosidase	924:936	arg1	activity					912:919	activity	912:919	activity of β-glucosidase	912:936	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	114	theme	β-glucosidase	924:936	arg1	abundance					947:955	abundance	947:955	abundance	947:955	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	114	theme	β-glucosidase	924:936	arg1	concentration					876:888	concentration	876:888	concentration	876:888	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	114	theme	β-glucosidase	924:936	arg1	diversity					958:966	diversity	958:966	diversity	958:966	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	5	114	theme	β-glucosidase	924:936	arg1	structure					894:902	structure	894:902	structure	894:902	The concentration and structure of DOM, activity of β-glucosidase, and the abundance, diversity, and community composition of GH1 (glycoside hydrolase family 1) microbial β-glucosidase encoding genes were investigated.					
33049534	7	115	from	concentration	1262:1274	arg1	aggregates					1295:1304	the soil aggregates	1286:1304	the soil aggregates	1286:1304	The concentration of DOM in the soil aggregates increased as the aggregate size decreased, while the structural complexity of DOM followed the opposite trend.					
33049534	0	116	from	roles	147:151	arg1	mineralization					171:184	organic matter mineralization	156:184	organic matter mineralization	156:184	Regulation of soil aggregate size under different fertilizations on dissolved organic matter, cellobiose hydrolyzing microbial community and their roles in organic matter mineralization.					
33049534	4	117	theme	long-term	762:770	arg1	Ck					789:790	Ck	789:790	Ck	789:790	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	117	theme	long-term	762:770	arg1	non-fertilizers					772:786	long-term non-fertilizers	762:786	long-term non-fertilizers (Ck)	762:791	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	3	118	theme	microbial	495:503	arg1	community					505:513	the cellobiose hydrolyzing microbial community	468:513	the cellobiose hydrolyzing microbial community	468:513	Regulation of soil aggregate size on dissolved organic matter (DOM), the cellobiose hydrolyzing microbial community, and their roles in organic matter mineralization remains unclear.					
33049534	4	119	from	microaggregates	702:716	arg1	mineralization					586:599	The mineralization	582:599	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM)	582:842	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	120	dep	macroaggregates	664:678	arg1	0.25-2 mm					686:694	0.25-2 mm	686:694	0.25-2 mm	686:694	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	4	120	dep	macroaggregates	664:678	arg1	SMA					681:683	SMA	681:683	SMA	681:683	The mineralization of organic matter in large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25-2 mm), and microaggregates (MI, <0.25 mm) from an Ultisol treated with long-term non-fertilizers (Ck), chemical fertilizers (NPK) and animal manure (AM) was observed in this study.					
33049534	10	121	theme	significant	1916:1926	arg1	differences					1928:1938	significant differences	1916:1938	significant differences among the aggregates	1916:1959	The relative abundance of Actinobacteria, Sordariomycetes, and Eurotiomycetes revealed significant differences among the aggregates.					
33049534	2	122	theme	organic	383:389	arg1	matter					391:396	soil organic matter	378:396	soil organic matter	378:396	Labile organic matter and microbial community are vital intrinsic factors in controlling the mineralization of soil organic matter.					
33049534	1	123	theme	climate	251:257	arg1	change					259:264	climate change	251:264	climate change	251:264	The mineralization of soil organic matter is closely related to climate change.					
34146805	5	0	theme	potential	991:999	arg1	hosts					1001:1005	the potential hosts	987:1005	the potential hosts of ARGs	987:1013	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	5	0	theme	potential	991:999	arg1	total					870:874	a total	868:874	a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes)	868:980	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	3	1	theme	bacterial	449:457	arg1	ARGs					443:446	71 ARGs	440:446	71 ARGs	440:446	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	3	1	theme	bacterial	449:457	arg1	communities					459:469	bacterial communities	449:469	bacterial communities	449:469	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	6	2	theme	ARGs	1135:1138	arg1	variation					1122:1130	the variation	1118:1130	the variation of ARGs	1118:1138	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	5	3	dep	Firmicutes	920:929	arg1	Proteobacteria					932:945	Proteobacteria	932:945	Proteobacteria	932:945	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	5	3	dep	Firmicutes	920:929	arg1	Bacteroidetes					967:979	Bacteroidetes	967:979	Bacteroidetes	967:979	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	5	3	dep	Firmicutes	920:929	arg1	Actinobacteria					948:961	Actinobacteria	948:961	Actinobacteria	948:961	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	4	4	theme	ARGs	770:773	arg1	abundance					713:721	abundance	713:721	abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs)	713:808	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	4	theme	ARGs	770:773	arg1	MGEs					804:807	MGEs	804:807	MGEs	804:807	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	4	theme	ARGs	770:773	arg1	amounts					696:702	amounts	696:702	amounts (18)	696:707	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	4	theme	ARGs	770:773	arg1	contents					646:653	antibiotics contents	634:653	antibiotics contents (CTC under detection limit, OFX 90.96%)	634:693	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	4	theme	ARGs	770:773	arg1	18					705:706	18	705:706	18	705:706	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	4	theme	ARGs	770:773	arg1	elements					794:801	mobile genetic elements	779:801	mobile genetic elements (MGEs)	779:808	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	4	theme	ARGs	770:773	arg1	ARGs					770:773	ARGs	770:773	ARGs	770:773	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	5	5	theme	Network	836:842	arg1	analysis					844:851	Network analysis	836:851	Network analysis	836:851	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	0	6	theme	antibiotics	82:92	arg1	removals					70:77	the removals	66:77	the removals of antibiotics and related resistance genes	66:121	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	4	7	dep	abundance	713:721	arg1	%					728:728	56.7%	724:728	56.7%	724:728	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	7	dep	abundance	713:721	arg1	%					735:735	11.1%	731:735	11.1% higher than zeolite addition	731:764	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	5	8	dep	phyla	913:917	arg1	Firmicutes					920:929	Firmicutes	920:929	Firmicutes	920:929	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	0	9	theme	resistance	106:115	arg1	genes					117:121	related resistance genes	98:121	related resistance genes	98:121	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	3	10	theme	ARGs	443:446	arg1	profiles					428:435	the profiles	424:435	the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX)	424:511	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	4	11	theme	higher	737:742	arg1	%					728:728	56.7%	724:728	56.7%	724:728	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	11	theme	higher	737:742	arg1	%					735:735	11.1%	731:735	11.1% higher than zeolite addition	731:764	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	12	dep	contents	646:653	arg1	%					692:692	OFX 90.96%	683:692	OFX 90.96%	683:692	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	12	dep	contents	646:653	arg1	CTC					656:658	CTC	656:658	CTC under detection limit	656:680	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	7	13	theme	physic-chemical	1216:1230	arg1	properties					1232:1241	physic-chemical properties	1216:1241	physic-chemical properties	1216:1241	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	2	14	theme	aerobic	361:367	arg1	composting					369:378	aerobic composting	361:378	aerobic composting	361:378	Recently, we have been searching a better solution to remove antibiotics and ARGs during aerobic composting.					
34146805	0	15	theme	related	98:104	arg1	genes					117:121	related resistance genes	98:121	related resistance genes	98:121	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	7	16	theme	ARG	1314:1316	arg1	transmission					1318:1329	ARG transmission	1314:1329	ARG transmission	1314:1329	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	8	17	from	composting	1404:1413	arg1	removal					1391:1397	removal	1391:1397	removal from composting	1391:1413	This study gives a new strategy about antibiotics and ARGs removal from composting on the basis of previous studies.					
34146805	3	18	theme	chitosan	403:410	arg1	addition					412:419	chitosan addition	403:419	chitosan addition	403:419	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	7	19	theme	transmission	1318:1329	arg1	risk					1306:1309	the risk	1302:1309	the risk of ARG transmission	1302:1329	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	4	20	theme	zeolite	749:755	arg1	addition					757:764	zeolite addition	749:764	zeolite addition	749:764	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	6	21	theme	main	1098:1101	arg1	succession					1080:1089	bacterial community succession	1060:1089	bacterial community succession	1060:1089	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	6	21	theme	main	1098:1101	arg1	contributor					1103:1113	the main contributor	1094:1113	the main contributor in the variation of ARGs	1094:1138	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	1	22	theme	antibiotic	179:188	arg1	ARGs					208:211	ARGs	208:211	ARGs	208:211	Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health.					
34146805	1	22	theme	antibiotic	179:188	arg1	genes					201:205	antibiotic resistance genes	179:205	antibiotic resistance genes (ARGs)	179:212	Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health.					
34146805	6	23	theme	bacterial	1060:1068	arg1	succession					1080:1089	bacterial community succession	1060:1089	bacterial community succession	1060:1089	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	6	23	theme	bacterial	1060:1068	arg1	contributor					1103:1113	the main contributor	1094:1113	the main contributor in the variation of ARGs	1094:1138	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	1	24	theme	endanger	249:256	arg1	health					264:269	endanger human health	249:269	soil and water as well as endanger human health	223:269	Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health.					
34146805	4	25	theme	OFX	683:685	arg1	%					692:692	OFX 90.96%	683:692	OFX 90.96%	683:692	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	25	theme	OFX	683:685	arg1	CTC					656:658	CTC	656:658	CTC under detection limit	656:680	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	1	26	theme	resistance	190:199	arg1	ARGs					208:211	ARGs	208:211	ARGs	208:211	Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health.					
34146805	1	26	theme	resistance	190:199	arg1	genes					201:205	antibiotic resistance genes	179:205	antibiotic resistance genes (ARGs)	179:212	Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health.					
34146805	1	27	theme	human	258:262	arg1	health					264:269	endanger human health	249:269	soil and water as well as endanger human health	223:269	Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health.					
34146805	7	28	theme	antibiotics	1268:1278	arg1	Zn2+					1287:1290	Zn2+	1287:1290	Zn2+	1287:1290	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	7	28	theme	antibiotics	1268:1278	arg1	Cu2+					1281:1284	Cu2+	1281:1284	Cu2+	1281:1284	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	7	28	theme	antibiotics	1268:1278	arg1	properties					1232:1241	physic-chemical properties	1216:1241	physic-chemical properties	1216:1241	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	7	28	theme	antibiotics	1268:1278	arg1	concentration					1251:1263	the concentration	1247:1263	the concentration of antibiotics	1247:1278	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	6	29	theme	Redundancy	1016:1025	arg1	RDA					1037:1039	RDA	1037:1039	RDA	1037:1039	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	6	29	theme	Redundancy	1016:1025	arg1	analysis					1027:1034	Redundancy analysis	1016:1034	Redundancy analysis (RDA)	1016:1040	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	0	30	theme	genes	117:121	arg1	removals					70:77	the removals	66:77	the removals of antibiotics and related resistance genes	66:121	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	7	31	theme	bacterial	1179:1187	arg1	composition					1189:1199	bacterial composition	1179:1199	bacterial composition	1179:1199	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	0	32	theme	bacterial	33:41	arg1	community					43:51	the bacterial community	29:51	the bacterial community	29:51	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	0	33	theme	manure	138:143	arg1	composting					145:154	chicken manure composting	130:154	chicken manure composting	130:154	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	7	34	theme	chitosan	1150:1157	arg1	addition					1159:1166	chitosan addition	1150:1166	chitosan addition	1150:1166	Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu2+, Zn2+ to reduce the risk of ARG transmission.					
34146805	5	35	theme	genera	882:887	arg1	strains					889:895	27 genera strains	879:895	27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes)	879:980	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	3	36	theme	addition	412:419	arg1	dynamics					391:398	the dynamics	387:398	the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX)	387:511	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	5	37	theme	ARGs	1010:1013	arg1	hosts					1001:1005	the potential hosts	987:1005	the potential hosts of ARGs	987:1013	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	5	37	theme	ARGs	1010:1013	arg1	total					870:874	a total	868:874	a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes)	868:980	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	0	38	theme	chicken	130:136	arg1	composting					145:154	chicken manure composting	130:154	chicken manure composting	130:154	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	3	39	theme	chicken	534:540	arg1	composting					549:558	chicken manure composting	534:558	chicken manure composting	534:558	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	4	40	theme	composting	824:833	arg1	days					819:822	42 days composting	816:833	42 days composting	816:833	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	8	41	theme	studies	1440:1446	arg1	basis					1422:1426	the basis	1418:1426	the basis of previous studies	1418:1446	This study gives a new strategy about antibiotics and ARGs removal from composting on the basis of previous studies.					
34146805	4	42	theme	mobile	779:784	arg1	MGEs					804:807	MGEs	804:807	MGEs	804:807	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	42	theme	mobile	779:784	arg1	elements					794:801	mobile genetic elements	779:801	mobile genetic elements (MGEs)	779:808	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	3	43	theme	zeolite	578:584	arg1	addition					586:593	zeolite addition	578:593	zeolite addition	578:593	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	4	44	theme	elements	794:801	arg1	abundance					713:721	abundance	713:721	abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs)	713:808	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	44	theme	elements	794:801	arg1	MGEs					804:807	MGEs	804:807	MGEs	804:807	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	44	theme	elements	794:801	arg1	amounts					696:702	amounts	696:702	amounts (18)	696:707	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	44	theme	elements	794:801	arg1	contents					646:653	antibiotics contents	634:653	antibiotics contents (CTC under detection limit, OFX 90.96%)	634:693	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	44	theme	elements	794:801	arg1	18					705:706	18	705:706	18	705:706	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	44	theme	elements	794:801	arg1	elements					794:801	mobile genetic elements	779:801	mobile genetic elements (MGEs)	779:808	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	44	theme	elements	794:801	arg1	ARGs					770:773	ARGs	770:773	ARGs	770:773	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	2	45	theme	better	307:312	arg1	solution					314:321	a better solution	305:321	a better solution to remove antibiotics and ARGs during aerobic composting	305:378	Recently, we have been searching a better solution to remove antibiotics and ARGs during aerobic composting.					
34146805	4	46	theme	detection	666:674	arg1	limit					676:680	detection limit	666:680	detection limit	666:680	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	0	47	dep	affects	21:27	arg1	accelerates					54:64	accelerates	54:64	accelerates the removals of antibiotics and related resistance genes during chicken manure composting	54:154	Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting.					
34146805	5	48	theme	strains	889:895	arg1	hosts					1001:1005	the potential hosts	987:1005	the potential hosts of ARGs	987:1013	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	5	48	theme	strains	889:895	arg1	total					870:874	a total	868:874	a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes)	868:980	Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs.					
34146805	4	49	theme	genetic	786:792	arg1	MGEs					804:807	MGEs	804:807	MGEs	804:807	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	49	theme	genetic	786:792	arg1	elements					794:801	mobile genetic elements	779:801	mobile genetic elements (MGEs)	779:808	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	8	50	theme	new	1351:1353	arg1	strategy					1355:1362	a new strategy	1349:1362	a new strategy about antibiotics and ARGs	1349:1389	This study gives a new strategy about antibiotics and ARGs removal from composting on the basis of previous studies.					
34146805	6	51	theme	community	1070:1078	arg1	succession					1080:1089	bacterial community succession	1060:1089	bacterial community succession	1060:1089	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	6	51	theme	community	1070:1078	arg1	contributor					1103:1113	the main contributor	1094:1113	the main contributor in the variation of ARGs	1094:1138	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	6	52	from	contributor	1103:1113	arg1	variation					1122:1130	the variation	1118:1130	the variation of ARGs	1118:1138	Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs.					
34146805	3	53	theme	manure	542:547	arg1	composting					549:558	chicken manure composting	534:558	chicken manure composting	534:558	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	3	54	from	dynamics	391:398	arg1	profiles					428:435	the profiles	424:435	the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX)	424:511	Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition.					
34146805	4	55	theme	Chitosan	596:603	arg1	addition					605:612	Chitosan addition	596:612	Chitosan addition	596:612	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	4	56	theme	antibiotics	634:644	arg1	contents					646:653	antibiotics contents	634:653	antibiotics contents (CTC under detection limit, OFX 90.96%)	634:693	Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting.					
34146805	8	57	theme	previous	1431:1438	arg1	studies					1440:1446	previous studies	1431:1446	previous studies	1431:1446	This study gives a new strategy about antibiotics and ARGs removal from composting on the basis of previous studies.					
34015382	6	0	dep	precipitates	1219:1230	arg1	addition					1181:1188	addition	1181:1188	addition	1181:1188	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	4	1	theme	precipitates	853:864	arg1	process					788:794	the RGF precipitation process	766:794	the RGF precipitation process as well as the physicochemical properties of the arising precipitates	766:864	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	4	1	theme	precipitates	853:864	arg1	properties					827:836	the physicochemical properties	807:836	the RGF precipitation process as well as the physicochemical properties of the arising precipitates	766:864	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	3	2	theme	acetate	702:708	arg1	HPMCAS					721:726	HPMCAS	721:726	HPMCAS	721:726	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	2	theme	acetate	702:708	arg1	succinate					710:718	hypromellose acetate succinate	689:718	hypromellose acetate succinate (HPMCAS)	689:727	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	6	3	theme	amorphous	1236:1244	arg1	co-precipitate					1246:1259	an amorphous co-precipitate	1233:1259	an amorphous co-precipitate of RGF and HPMCAS	1233:1277	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	3	4	theme	RGF	535:537	arg1	monohydrate					522:532	Regorafenib monohydrate	510:532	Regorafenib monohydrate (RGF MH)	510:541	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	4	theme	RGF	535:537	arg1	MH					539:540	RGF MH	535:540	RGF MH	535:540	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	5	theme	inhibitor	558:566	arg1	monohydrate					522:532	Regorafenib monohydrate	510:532	Regorafenib monohydrate (RGF MH)	510:541	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	5	theme	inhibitor	558:566	arg1	drug					568:571	a multikinase inhibitor drug	544:571	a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound	544:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	9	6	theme	environmental	1606:1618	arg1	conditions					1620:1629	the environmental conditions	1602:1629	the environmental conditions	1602:1629	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	6	7	theme	RGF	1215:1217	arg1	precipitates					1219:1230	different crystalline RGF precipitates	1193:1230	different crystalline RGF precipitates	1193:1230	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	1	8	theme	formulation	168:178	arg1	technique					180:188	a major drug formulation technique	155:188	a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients	155:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	8	theme	formulation	168:178	arg1	Dispersions					132:142	Amorphous Solid Dispersions	116:142	Amorphous Solid Dispersions (ASDs)	116:149	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	7	9	theme	X-ray	1351:1355	arg1	WAXS					1369:1372	WAXS	1369:1372	WAXS	1369:1372	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	9	theme	X-ray	1351:1355	arg1	scattering					1357:1366	Wide angle X-ray scattering	1340:1366	Wide angle X-ray scattering (WAXS)	1340:1373	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	6	10	theme	different	1193:1201	arg1	precipitates					1219:1230	different crystalline RGF precipitates	1193:1230	different crystalline RGF precipitates	1193:1230	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	3	11	theme	Biopharmaceutics	588:603	arg1	BCS					628:630	BCS	628:630	BCS	628:630	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	11	theme	Biopharmaceutics	588:603	arg1	System					620:625	Biopharmaceutics Classification System	588:625	Biopharmaceutics Classification System (BCS) class II compound	588:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	4	12	theme	arising	845:851	arg1	precipitates					853:864	the arising precipitates	841:864	the arising precipitates	841:864	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	7	13	theme	Wide	1340:1343	arg1	WAXS					1369:1372	WAXS	1369:1372	WAXS	1369:1372	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	13	theme	Wide	1340:1343	arg1	scattering					1357:1366	Wide angle X-ray scattering	1340:1366	Wide angle X-ray scattering (WAXS)	1340:1373	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	2	14	theme	supersaturation	318:332	arg1	enhancement					334:344	supersaturation enhancement	318:344	supersaturation enhancement	318:344	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	1	15	theme	higher	201:206	arg1	bioavailability					208:222	higher bioavailability	201:222	higher bioavailability	201:222	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	5	16	theme	confocal	1071:1078	arg1	CRM					1098:1100	CRM	1098:1100	CRM	1098:1100	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	16	theme	confocal	1071:1078	arg1	microscopy					1086:1095	confocal Raman microscopy	1071:1095	confocal Raman microscopy (CRM)	1071:1101	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	17	theme	scanning	1043:1050	arg1	DSC					1065:1067	DSC	1065:1067	DSC	1065:1067	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	17	theme	scanning	1043:1050	arg1	calorimetry					1052:1062	differential scanning calorimetry	1030:1062	differential scanning calorimetry (DSC)	1030:1068	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	3	18	theme	System	620:625	arg1	compound					642:649	Biopharmaceutics Classification System (BCS) class II compound	588:649	Biopharmaceutics Classification System (BCS) class II compound	588:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	5	19	theme	biorelevant	913:923	arg1	dissolution					925:935	biorelevant dissolution	913:935	biorelevant dissolution	913:935	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	10	20	theme	formulation	1787:1797	arg1	formation					1706:1714	The engineered formation	1691:1714	The engineered formation of defined amorphous structures in-vivo	1691:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	20	theme	formulation	1787:1797	arg1	strategy					1799:1806	a promising future drug formulation strategy	1763:1806	a promising future drug formulation strategy	1763:1806	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	3	21	theme	class	633:637	arg1	compound					642:649	Biopharmaceutics Classification System (BCS) class II compound	588:649	Biopharmaceutics Classification System (BCS) class II compound	588:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	5	22	theme	powder	1110:1115	arg1	XRPD					1130:1133	XRPD	1130:1133	XRPD	1130:1133	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	22	theme	powder	1110:1115	arg1	diffraction					1117:1127	X-ray powder diffraction	1104:1127	X-ray powder diffraction (XRPD)	1104:1134	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	1	23	theme	major	157:161	arg1	technique					180:188	a major drug formulation technique	155:188	a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients	155:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	23	theme	major	157:161	arg1	Dispersions					132:142	Amorphous Solid Dispersions	116:142	Amorphous Solid Dispersions (ASDs)	116:149	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	0	24	from	Precipitation	0:12	arg1	testing					74:80	biorelevant dissolution testing	50:80	biorelevant dissolution testing	50:80	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	4	25	theme	first	754:758	arg1	time					760:763	the first time	750:763	the first time	750:763	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	5	26	from	dissolution	925:935	arg1	precipitates					895:906	The formed precipitates	884:906	The formed precipitates from biorelevant dissolution	884:935	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	0	27	from	dispersions	35:45	arg1	Precipitation					0:12	Precipitation	0:12	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.	0:114	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	6	28	dep	PVP	1335:1337	arg1	the					1319:1321	the	1319:1321	the	1319:1321	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	6	28	dep	PVP	1335:1337	arg1	presence					1323:1330	presence	1323:1330	presence	1323:1330	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	5	29	theme	drug	952:955	arg1	content					957:963	varying drug content	944:963	varying drug content	944:963	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	0	30	theme	amorphous	19:27	arg1	dispersions					35:45	amorphous solid dispersions	19:45	amorphous solid dispersions	19:45	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	5	31	theme	angle	1147:1151	arg1	SAXS					1171:1174	SAXS	1171:1174	SAXS	1171:1174	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	31	theme	angle	1147:1151	arg1	scattering					1159:1168	small angle X-ray scattering	1141:1168	small angle X-ray scattering (SAXS)	1141:1175	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	10	32	theme	engineered	1695:1704	arg1	formation					1706:1714	The engineered formation	1691:1714	The engineered formation of defined amorphous structures in-vivo	1691:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	32	theme	engineered	1695:1704	arg1	strategy					1799:1806	a promising future drug formulation strategy	1763:1806	a promising future drug formulation strategy	1763:1806	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	1	33	theme	Amorphous	116:124	arg1	technique					180:188	a major drug formulation technique	155:188	a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients	155:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	33	theme	Amorphous	116:124	arg1	ASDs					145:148	ASDs	145:148	ASDs	145:148	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	33	theme	Amorphous	116:124	arg1	Dispersions					132:142	Amorphous Solid Dispersions	116:142	Amorphous Solid Dispersions (ASDs)	116:149	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	2	34	theme	drug	493:496	arg1	absorption					498:507	drug absorption	493:507	drug absorption	493:507	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	2	35	theme	precipitates	428:439	arg1	importance					407:416	the importance	403:416	the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption	403:507	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	2	36	dep	amorphous	446:454	arg1	states					471:476	states	471:476	states	471:476	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	3	37	theme	Regorafenib	510:520	arg1	monohydrate					522:532	Regorafenib monohydrate	510:532	Regorafenib monohydrate (RGF MH)	510:541	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	37	theme	Regorafenib	510:520	arg1	MH					539:540	RGF MH	535:540	RGF MH	535:540	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	37	theme	Regorafenib	510:520	arg1	drug					568:571	a multikinase inhibitor drug	544:571	a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound	544:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	37	theme	Regorafenib	510:520	arg1	ASD					735:737	an ASD	732:737	an ASD	732:737	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	0	38	theme	biorelevant	50:60	arg1	testing					74:80	biorelevant dissolution testing	50:80	biorelevant dissolution testing	50:80	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	7	39	theme	precipitation	1431:1443	arg1	process					1445:1451	the precipitation process	1427:1451	the precipitation process of RGF in-situ	1427:1466	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	5	40	theme	differential	1030:1041	arg1	DSC					1065:1067	DSC	1065:1067	DSC	1065:1067	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	40	theme	differential	1030:1041	arg1	calorimetry					1052:1062	differential scanning calorimetry	1030:1062	differential scanning calorimetry (DSC)	1030:1068	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	10	41	theme	future	1775:1780	arg1	formation					1706:1714	The engineered formation	1691:1714	The engineered formation of defined amorphous structures in-vivo	1691:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	41	theme	future	1775:1780	arg1	strategy					1799:1806	a promising future drug formulation strategy	1763:1806	a promising future drug formulation strategy	1763:1806	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	3	42	theme	hypromellose	689:700	arg1	HPMCAS					721:726	HPMCAS	721:726	HPMCAS	721:726	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	42	theme	hypromellose	689:700	arg1	succinate					710:718	hypromellose acetate succinate	689:718	hypromellose acetate succinate (HPMCAS)	689:727	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	4	43	theme	RGF	770:772	arg1	process					788:794	the RGF precipitation process	766:794	the RGF precipitation process as well as the physicochemical properties of the arising precipitates	766:864	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	5	44	theme	electron	1003:1010	arg1	microscopy					1012:1021	scanning electron microscopy	994:1021	scanning electron microscopy (SEM)	994:1027	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	44	theme	electron	1003:1010	arg1	SEM					1024:1026	SEM	1024:1026	SEM	1024:1026	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	1	45	theme	active	249:254	arg1	ingredients					271:281	poorly water-soluble active pharmaceutical ingredients	228:281	poorly water-soluble active pharmaceutical ingredients	228:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	9	46	theme	polymorphic	1570:1580	arg1	states					1582:1587	multiple polymorphic states	1561:1587	multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients	1561:1688	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	7	47	used	used	1413:1416	arg2	ITC					1403:1405	ITC	1403:1405	ITC	1403:1405	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	47	used	used	1413:1416	arg2	WAXS					1369:1372	WAXS	1369:1372	WAXS	1369:1372	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	47	used	used	1413:1416	arg2	scattering					1357:1366	Wide angle X-ray scattering	1340:1366	Wide angle X-ray scattering (WAXS)	1340:1373	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	47	used	used	1413:1416	arg2	calorimetry					1390:1400	isothermal calorimetry	1379:1400	isothermal calorimetry (ITC)	1379:1406	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	9	48	theme	dependent	1589:1597	arg1	states					1582:1587	multiple polymorphic states	1561:1587	multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients	1561:1688	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	3	49	theme	multikinase	546:556	arg1	monohydrate					522:532	Regorafenib monohydrate	510:532	Regorafenib monohydrate (RGF MH)	510:541	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	49	theme	multikinase	546:556	arg1	drug					568:571	a multikinase inhibitor drug	544:571	a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound	544:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	2	50	with	precipitates	428:439	arg1	crystalline					459:469	crystalline	459:469	crystalline	459:469	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	2	50	with	precipitates	428:439	arg1	amorphous					446:454	amorphous	446:454	amorphous	446:454	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	2	51	theme	dissolution	292:302	arg1	tailoring					304:312	dissolution tailoring	292:312	dissolution tailoring	292:312	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	1	52	theme	drug	163:166	arg1	technique					180:188	a major drug formulation technique	155:188	a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients	155:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	52	theme	drug	163:166	arg1	Dispersions					132:142	Amorphous Solid Dispersions	116:142	Amorphous Solid Dispersions (ASDs)	116:149	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	7	53	theme	isothermal	1379:1388	arg1	ITC					1403:1405	ITC	1403:1405	ITC	1403:1405	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	53	theme	isothermal	1379:1388	arg1	calorimetry					1390:1400	isothermal calorimetry	1379:1400	isothermal calorimetry (ITC)	1379:1406	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	4	54	theme	physicochemical	811:825	arg1	properties					827:836	the physicochemical properties	807:836	the RGF precipitation process as well as the physicochemical properties of the arising precipitates	766:864	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	6	55	theme	crystalline	1203:1213	arg1	precipitates					1219:1230	different crystalline RGF precipitates	1193:1230	different crystalline RGF precipitates	1193:1230	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	10	56	theme	in-vivo	1748:1754	arg1	structures					1737:1746	defined amorphous structures in-vivo	1719:1754	defined amorphous structures in-vivo	1719:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	57	theme	amorphous	1727:1735	arg1	structures					1737:1746	defined amorphous structures in-vivo	1719:1754	defined amorphous structures in-vivo	1719:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	58	theme	structures	1737:1746	arg1	formation					1706:1714	The engineered formation	1691:1714	The engineered formation of defined amorphous structures in-vivo	1691:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	58	theme	structures	1737:1746	arg1	strategy					1799:1806	a promising future drug formulation strategy	1763:1806	a promising future drug formulation strategy	1763:1806	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	9	59	theme	dissolution	1638:1648	arg1	media					1650:1654	dissolution media	1638:1654	dissolution media	1638:1654	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	7	60	theme	angle	1345:1349	arg1	WAXS					1369:1372	WAXS	1369:1372	WAXS	1369:1372	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	7	60	theme	angle	1345:1349	arg1	scattering					1357:1366	Wide angle X-ray scattering	1340:1366	Wide angle X-ray scattering (WAXS)	1340:1373	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	10	61	theme	promising	1765:1773	arg1	formation					1706:1714	The engineered formation	1691:1714	The engineered formation of defined amorphous structures in-vivo	1691:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	61	theme	promising	1765:1773	arg1	strategy					1799:1806	a promising future drug formulation strategy	1763:1806	a promising future drug formulation strategy	1763:1806	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	6	62	theme	HPMCAS	1272:1277	arg1	co-precipitate					1246:1259	an amorphous co-precipitate	1233:1259	an amorphous co-precipitate of RGF and HPMCAS	1233:1277	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	5	63	theme	Raman	1080:1084	arg1	CRM					1098:1100	CRM	1098:1100	CRM	1098:1100	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	63	theme	Raman	1080:1084	arg1	microscopy					1086:1095	confocal Raman microscopy	1071:1095	confocal Raman microscopy (CRM)	1071:1101	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	3	64	theme	Classification	605:618	arg1	BCS					628:630	BCS	628:630	BCS	628:630	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	3	64	theme	Classification	605:618	arg1	System					620:625	Biopharmaceutics Classification System	588:625	Biopharmaceutics Classification System (BCS) class II compound	588:649	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	1	65	theme	Solid	126:130	arg1	technique					180:188	a major drug formulation technique	155:188	a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients	155:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	65	theme	Solid	126:130	arg1	ASDs					145:148	ASDs	145:148	ASDs	145:148	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	1	65	theme	Solid	126:130	arg1	Dispersions					132:142	Amorphous Solid Dispersions	116:142	Amorphous Solid Dispersions (ASDs)	116:149	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	9	66	theme	chosen	1672:1677	arg1	excipients					1679:1688	chosen excipients	1672:1688	chosen excipients	1672:1688	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	6	67	theme	RGF	1264:1266	arg1	co-precipitate					1246:1259	an amorphous co-precipitate	1233:1259	an amorphous co-precipitate of RGF and HPMCAS	1233:1277	In addition to different crystalline RGF precipitates, an amorphous co-precipitate of RGF and HPMCAS was identified, which was suppressed in the presence of PVP.					
34015382	9	68	dep	media	1650:1654	arg1	composition					1656:1666	composition	1656:1666	composition	1656:1666	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	9	68	dep	media	1650:1654	arg1	excipients					1679:1688	chosen excipients	1672:1688	chosen excipients	1672:1688	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	9	68	dep	media	1650:1654	arg1	i.e.					1632:1635	i.e.	1632:1635	i.e.	1632:1635	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	10	69	theme	drug	1782:1785	arg1	formation					1706:1714	The engineered formation	1691:1714	The engineered formation of defined amorphous structures in-vivo	1691:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	10	69	theme	drug	1782:1785	arg1	strategy					1799:1806	a promising future drug formulation strategy	1763:1806	a promising future drug formulation strategy	1763:1806	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	5	70	theme	X-ray	1104:1108	arg1	XRPD					1130:1133	XRPD	1130:1133	XRPD	1130:1133	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	70	theme	X-ray	1104:1108	arg1	diffraction					1117:1127	X-ray powder diffraction	1104:1127	X-ray powder diffraction (XRPD)	1104:1134	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	71	theme	formed	888:893	arg1	precipitates					895:906	The formed precipitates	884:906	The formed precipitates from biorelevant dissolution	884:935	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	2	72	theme	absorption	498:507	arg1	site					485:488	the site	481:488	the site of drug absorption	481:507	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	2	72	theme	absorption	498:507	arg1	absorption					498:507	drug absorption	493:507	drug absorption	493:507	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	0	73	theme	regorafenib	103:113	arg1	polymorphism					87:98	The polymorphism	83:98	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.	0:114	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	10	74	theme	defined	1719:1725	arg1	structures					1737:1746	defined amorphous structures in-vivo	1719:1754	defined amorphous structures in-vivo	1719:1754	The engineered formation of defined amorphous structures in-vivo may be a promising future drug formulation strategy.					
34015382	2	75	from	site	485:488	arg1	importance					407:416	the importance	403:416	the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption	403:507	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	1	76	theme	water-soluble	235:247	arg1	ingredients					271:281	poorly water-soluble active pharmaceutical ingredients	228:281	poorly water-soluble active pharmaceutical ingredients	228:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
34015382	5	77	theme	varying	944:950	arg1	content					957:963	varying drug content	944:963	varying drug content	944:963	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	8	78	theme	calorimetric	1474:1485	arg1	data					1487:1490	calorimetric data	1474:1490	calorimetric data	1474:1490	From calorimetric data, the precipitation profile was calculated.					
34015382	2	79	dep	studied	356:362	arg1	whereas					375:381	whereas	375:381	whereas	375:381	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	0	80	theme	solid	29:33	arg1	dispersions					35:45	amorphous solid dispersions	19:45	amorphous solid dispersions	19:45	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	5	81	theme	small	1141:1145	arg1	SAXS					1171:1174	SAXS	1171:1174	SAXS	1171:1174	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	81	theme	small	1141:1145	arg1	scattering					1159:1168	small angle X-ray scattering	1141:1168	small angle X-ray scattering (SAXS)	1141:1175	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	82	theme	X-ray	1153:1157	arg1	SAXS					1171:1174	SAXS	1171:1174	SAXS	1171:1174	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	82	theme	X-ray	1153:1157	arg1	scattering					1159:1168	small angle X-ray scattering	1141:1168	small angle X-ray scattering (SAXS)	1141:1175	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	8	83	theme	precipitation	1497:1509	arg1	profile					1511:1517	the precipitation profile	1493:1517	the precipitation profile	1493:1517	From calorimetric data, the precipitation profile was calculated.					
34015382	2	84	theme	formed	421:426	arg1	precipitates					428:439	formed precipitates	421:439	formed precipitates with amorphous or crystalline states	421:476	So far, dissolution tailoring and supersaturation enhancement have been studied in detail, whereas less is known about the importance of formed precipitates with amorphous or crystalline states at the site of drug absorption.					
34015382	0	85	theme	dissolution	62:72	arg1	testing					74:80	biorelevant dissolution testing	50:80	biorelevant dissolution testing	50:80	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	7	86	theme	RGF	1456:1458	arg1	process					1445:1451	the precipitation process	1427:1451	the precipitation process of RGF in-situ	1427:1466	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	0	87	dep	Precipitation	0:12	arg1	polymorphism					87:98	The polymorphism	83:98	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.	0:114	Precipitation from amorphous solid dispersions in biorelevant dissolution testing: The polymorphism of regorafenib.					
34015382	5	88	theme	scanning	994:1001	arg1	microscopy					1012:1021	scanning electron microscopy	994:1021	scanning electron microscopy (SEM)	994:1027	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	5	88	theme	scanning	994:1001	arg1	SEM					1024:1026	SEM	1024:1026	SEM	1024:1026	The formed precipitates from biorelevant dissolution showed varying drug content and were analyzed offline by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), confocal Raman microscopy (CRM), X-ray powder diffraction (XRPD), and small angle X-ray scattering (SAXS).					
34015382	3	89	theme	povidone	672:679	arg1	K25					681:683	povidone K25	672:683	povidone K25	672:683	Regorafenib monohydrate (RGF MH), a multikinase inhibitor drug categorized as Biopharmaceutics Classification System (BCS) class II compound, was formulated with povidone K25 and hypromellose acetate succinate (HPMCAS) as an ASD.					
34015382	7	90	dep	RGF	1456:1458	arg1	in-situ					1460:1466	RGF in-situ	1456:1466	RGF in-situ	1456:1466	Wide angle X-ray scattering (WAXS) and isothermal calorimetry (ITC) were used to track the precipitation process of RGF in-situ.					
34015382	9	91	theme	multiple	1561:1568	arg1	states					1582:1587	multiple polymorphic states	1561:1587	multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients	1561:1688	RGF forms precipitates in multiple polymorphic states dependent on the environmental conditions, i.e., dissolution media composition and chosen excipients.					
34015382	4	92	theme	precipitation	774:786	arg1	process					788:794	the RGF precipitation process	766:794	the RGF precipitation process as well as the physicochemical properties of the arising precipitates	766:864	Here, for the first time, the RGF precipitation process as well as the physicochemical properties of the arising precipitates are investigated.					
34015382	1	93	theme	pharmaceutical	256:269	arg1	ingredients					271:281	poorly water-soluble active pharmaceutical ingredients	228:281	poorly water-soluble active pharmaceutical ingredients	228:281	Amorphous Solid Dispersions (ASDs) are a major drug formulation technique to achieve higher bioavailability for poorly water-soluble active pharmaceutical ingredients.					
33434031	3	0	link	Melibiose-derived	952:968	arg1	verbascotetraose					1023:1038	verbascotetraose	1023:1038	verbascotetraose	1023:1038	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	3	0	link	Melibiose-derived	952:968	arg1	α-GOS					997:1001	α-GOS	997:1001	α-GOS	997:1001	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	3	0	link	Melibiose-derived	952:968	arg1	manninotriose					1005:1017	manninotriose	1005:1017	manninotriose	1005:1017	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	3	0	link	Melibiose-derived	952:968	arg1	α-galactooligosaccharides					970:994	Melibiose-derived α-galactooligosaccharides	952:994	Melibiose-derived α-galactooligosaccharides (α-GOS)	952:1002	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	2	1	attach	derived	576:582	arg2	products					567:574	the transfer products	554:574	the transfer products derived from melibiose:lactulose reaction mixtures	554:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	1	attach	derived	576:582	arg1	mixtures					618:625	melibiose:lactulose reaction mixtures	589:625	melibiose:lactulose reaction mixtures	589:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	2	theme	Lactobacillus	197:209	arg1	WCFS1					221:225	Lactobacillus plantarum WCFS1	197:225	Lactobacillus plantarum WCFS1	197:225	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	3	theme	disaccharide-acceptors	302:323	arg1	group					293:297	the C6-hydroxyl group	277:297	the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS)	277:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	3	theme	disaccharide-acceptors	302:323	arg1	disaccharide-acceptors					302:323	disaccharide-acceptors	302:323	disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose)	302:420	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	2	4	theme	products	567:574	arg1	characterization					534:549	A comprehensive nuclear magnetic resonance characterization	491:549	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures	491:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	5	theme	-β-d-galactopyranosyl-	684:705	arg1	biosynthesis					640:651	the biosynthesis	636:651	the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	636:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	6	theme	main	734:737	arg1	component					739:747	the main component	730:747	the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	730:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	7	theme	plantarum	211:219	arg1	WCFS1					221:225	Lactobacillus plantarum WCFS1	197:225	Lactobacillus plantarum WCFS1	197:225	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	4	8	theme	digestibility	1120:1132	arg1	assessment					1091:1100	An in vitro assessment	1079:1100	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS	1079:1164	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	5	9	theme	microbiota	1493:1502	arg1	composition					1504:1514	the gut microbiota composition	1485:1514	the gut microbiota composition	1485:1514	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	5	10	theme	conventional	1313:1324	arg1	α-GOS					1326:1330	conventional α-GOS	1313:1330	conventional α-GOS	1313:1330	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	5	11	theme	other	1523:1527	arg1	applications					1529:1540	other applications	1523:1540	other applications	1523:1540	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	5	11	theme	other	1523:1527	arg1	ingredients					1568:1578	low-calorie food ingredients	1551:1578	low-calorie food ingredients	1551:1578	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	4	12	theme	intestinal	1109:1118	arg1	digestibility					1120:1132	the intestinal digestibility	1105:1132	the intestinal digestibility of the novel biosynthesized HOS	1105:1164	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	2	13	theme	-β-d-galactopyranosyl-	908:929	arg1	presence					764:771	the presence	760:771	the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	730:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	13	theme	-β-d-galactopyranosyl-	908:929	arg1	component					739:747	the main component	730:747	the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	730:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	14	theme	reaction	609:616	arg1	mixtures					618:625	melibiose:lactulose reaction mixtures	589:625	melibiose:lactulose reaction mixtures	589:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	15	dep	-α-d-galactopyranosyl-	879:900	arg1	→					904:904	1 → 6	902:906	1 → 6	902:906	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	15	dep	-α-d-galactopyranosyl-	879:900	arg1	→					875:875	1 → 6	873:877	1 → 6	873:877	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	4	16	theme	high	1177:1180	arg1	resistance					1182:1191	a high resistance	1175:1191	a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose	1175:1271	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	2	17	theme	lactulose	599:607	arg1	mixtures					618:625	melibiose:lactulose reaction mixtures	589:625	melibiose:lactulose reaction mixtures	589:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	18	theme	-β-d-fructose	713:725	arg1	-β-d-galactopyranosyl-					684:705	α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)	656:712	α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	656:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	18	theme	-β-d-fructose	713:725	arg1	→					680:680	1 → 6	678:682	1 → 6	678:682	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	5	19	theme	novel	1396:1400	arg1	α-galactosides					1416:1429	these novel nondigestible α-galactosides	1390:1429	these novel nondigestible α-galactosides	1390:1429	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	5	20	theme	gut	1489:1491	arg1	composition					1504:1514	the gut microbiota composition	1485:1514	the gut microbiota composition	1485:1514	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	5	21	theme	nondigestible	1402:1414	arg1	α-galactosides					1416:1429	these novel nondigestible α-galactosides	1390:1429	these novel nondigestible α-galactosides	1390:1429	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	2	22	theme	resonance	524:532	arg1	characterization					534:549	A comprehensive nuclear magnetic resonance characterization	491:549	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures	491:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	4	23	dep	in	1082:1083	arg1	vitro					1085:1089	vitro	1085:1089	vitro	1085:1089	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	2	24	theme	melibiose	589:597	arg1	mixtures					618:625	melibiose:lactulose reaction mixtures	589:625	melibiose:lactulose reaction mixtures	589:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	4	25	theme	α-galactosides	1196:1209	arg1	resistance					1182:1191	a high resistance	1175:1191	a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose	1175:1271	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	2	26	theme	magnetic	515:522	arg1	resonance					524:532	A comprehensive nuclear magnetic resonance	491:532	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures	491:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	5	27	theme	food	1563:1566	arg1	ingredients					1568:1578	low-calorie food ingredients	1551:1578	low-calorie food ingredients	1551:1578	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	2	28	theme	-β-d-fructose	833:845	arg1	presence					764:771	the presence	760:771	the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	730:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	28	theme	-β-d-fructose	833:845	arg1	component					739:747	the main component	730:747	the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	730:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	29	theme	galactosyl	239:248	arg1	residues					250:257	galactosyl residues	239:257	galactosyl residues	239:257	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	0	30	theme	Galactose-Containing	30:49	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of Nondigestible Galactose-Containing	0:49	Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.					
33434031	1	31	from	capacity	159:166	arg1	WCFS1					221:225	Lactobacillus plantarum WCFS1	197:225	Lactobacillus plantarum WCFS1	197:225	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	4	32	theme	HOS	1162:1164	arg1	digestibility					1120:1132	the intestinal digestibility	1105:1132	the intestinal digestibility of the novel biosynthesized HOS	1105:1164	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	0	33	theme	Nondigestible	16:28	arg1	Galactose-Containing					30:49	Nondigestible Galactose-Containing	16:49	Nondigestible Galactose-Containing	16:49	Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.					
33434031	1	34	dep	α-linkages	381:390	arg1	isomaltulose					393:404	isomaltulose	393:404	isomaltulose	393:404	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	34	dep	α-linkages	381:390	arg1	α-linkages					381:390	α-linkages	381:390	α-linkages (isomaltulose and isomaltose)	381:420	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	34	dep	α-linkages	381:390	arg1	isomaltose					410:419	isomaltose	410:419	isomaltose	410:419	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	2	35	dep	-β-d-fructose	833:845	arg1	-β-d-fructose					937:949	-β-d-fructose	937:949	-β-d-fructose	937:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	36	theme	novel	433:437	arg1	hetero-oligosaccharides					460:482	novel galactose-containing hetero-oligosaccharides	433:482	novel galactose-containing hetero-oligosaccharides (HOS)	433:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	36	theme	novel	433:437	arg1	HOS					485:487	HOS	485:487	HOS	485:487	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	2	37	dep	α-d-galactopyranosyl-	776:796	arg1	-β-d-galactopyranosyl-					804:825	1 → 3)-β-d-galactopyranosyl-(1 → 4	798:831	1 → 3)-β-d-galactopyranosyl-(1 → 4	798:831	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	38	theme	transfer	558:565	arg1	products					567:574	the transfer products	554:574	the transfer products derived from melibiose:lactulose reaction mixtures	554:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	39	theme	galactose-containing	439:458	arg1	hetero-oligosaccharides					460:482	novel galactose-containing hetero-oligosaccharides	433:482	novel galactose-containing hetero-oligosaccharides (HOS)	433:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	39	theme	galactose-containing	439:458	arg1	HOS					485:487	HOS	485:487	HOS	485:487	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	3	40	theme	Melibiose-derived	952:968	arg1	verbascotetraose					1023:1038	verbascotetraose	1023:1038	verbascotetraose	1023:1038	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	3	40	theme	Melibiose-derived	952:968	arg1	α-GOS					997:1001	α-GOS	997:1001	α-GOS	997:1001	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	3	40	theme	Melibiose-derived	952:968	arg1	manninotriose					1005:1017	manninotriose	1005:1017	manninotriose	1005:1017	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	3	40	theme	Melibiose-derived	952:968	arg1	α-galactooligosaccharides					970:994	Melibiose-derived α-galactooligosaccharides	952:994	Melibiose-derived α-galactooligosaccharides (α-GOS)	952:1002	Melibiose-derived α-galactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized.					
33434031	4	41	attach	derived	1211:1217	arg1	lactose					1235:1241	lactose	1235:1241	lactose	1235:1241	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	4	41	attach	derived	1211:1217	arg1	cellobiose					1244:1253	cellobiose	1244:1253	cellobiose	1244:1253	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	4	41	attach	derived	1211:1217	arg1	isomaltulose					1260:1271	isomaltulose	1260:1271	isomaltulose	1260:1271	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	4	41	attach	derived	1211:1217	arg2	α-galactosides					1196:1209	α-galactosides	1196:1209	α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose	1196:1271	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	4	41	attach	derived	1211:1217	arg1	lactulose					1224:1232	lactulose	1224:1232	lactulose	1224:1232	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	2	42	theme	nuclear	507:513	arg1	resonance					524:532	A comprehensive nuclear magnetic resonance	491:532	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures	491:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	43	theme	α-d-galactopyranosyl-	776:796	arg1	-β-d-fructose					833:845	α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	776:845	α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	776:845	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	0	44	theme	plantarum	92:100	arg1	α-Galactosidase					113:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.					
33434031	1	45	theme	high	154:157	arg1	capacity					159:166	the high capacity	150:166	the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS)	150:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	0	46	theme	Lactobacillus	78:90	arg1	α-Galactosidase					113:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.					
33434031	1	47	dep	β-linkages	330:339	arg1	lactose					353:359	lactose	353:359	lactose	353:359	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	47	dep	β-linkages	330:339	arg1	cellobiose					366:375	cellobiose	366:375	cellobiose	366:375	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	47	dep	β-linkages	330:339	arg1	lactulose					342:350	lactulose	342:350	lactulose	342:350	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	47	dep	β-linkages	330:339	arg1	β-linkages					330:339	β-linkages	330:339	β-linkages (lactulose, lactose, and cellobiose)	330:376	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	cellobiose					366:375	cellobiose	366:375	cellobiose	366:375	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	isomaltose					410:419	isomaltose	410:419	isomaltose	410:419	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	lactulose					342:350	lactulose	342:350	lactulose	342:350	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	lactose					353:359	lactose	353:359	lactose	353:359	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	α-linkages					381:390	α-linkages	381:390	α-linkages (isomaltulose and isomaltose)	381:420	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	isomaltulose					393:404	isomaltulose	393:404	isomaltulose	393:404	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	48	with	disaccharide-acceptors	302:323	arg1	β-linkages					330:339	β-linkages	330:339	β-linkages (lactulose, lactose, and cellobiose)	330:376	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	5	49	theme	low-calorie	1551:1561	arg1	ingredients					1568:1578	low-calorie food ingredients	1551:1578	low-calorie food ingredients	1551:1578	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	5	50	theme	potential	1440:1448	arg1	candidates					1450:1459	potential candidates	1440:1459	potential candidates	1440:1459	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	2	51	theme	α-d-galactopyranosyl-	656:676	arg1	-β-d-galactopyranosyl-					684:705	α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)	656:712	α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose	656:949	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	51	theme	α-d-galactopyranosyl-	656:676	arg1	→					680:680	1 → 6	678:682	1 → 6	678:682	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	52	dep	-β-d-galactopyranosyl-	804:825	arg1	3					802:802	3	802:802	3	802:802	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	53	theme	MelA	171:174	arg1	α-galactosidase					176:190	MelA α-galactosidase	171:190	MelA α-galactosidase from Lactobacillus plantarum WCFS1	171:225	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	4	54	theme	biosynthesized	1147:1160	arg1	HOS					1162:1164	the novel biosynthesized HOS	1137:1164	the novel biosynthesized HOS	1137:1164	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	1	55	theme	C6-hydroxyl	281:291	arg1	group					293:297	the C6-hydroxyl group	277:297	the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS)	277:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	55	theme	C6-hydroxyl	281:291	arg1	disaccharide-acceptors					302:323	disaccharide-acceptors	302:323	disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose)	302:420	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	0	56	theme	MelA	108:111	arg1	α-Galactosidase					113:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.					
33434031	5	57	theme	certain	1336:1342	arg1	disaccharides					1344:1356	certain disaccharides	1336:1356	certain disaccharides	1336:1356	According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate the gut microbiota composition, among other applications, such as low-calorie food ingredients.					
33434031	2	58	theme	→	800:800	arg1	-β-d-galactopyranosyl-					804:825	1 → 3)-β-d-galactopyranosyl-(1 → 4	798:831	1 → 3)-β-d-galactopyranosyl-(1 → 4	798:831	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	59	theme	α-galactosidase	176:190	arg1	capacity					159:166	the high capacity	150:166	the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS)	150:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	4	60	theme	novel	1141:1145	arg1	HOS					1162:1164	the novel biosynthesized HOS	1137:1164	the novel biosynthesized HOS	1137:1164	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	0	61	theme	WCFS1	102:106	arg1	α-Galactosidase					113:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Lactobacillus plantarum WCFS1 MelA α-Galactosidase	78:127	Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.					
33434031	2	62	theme	comprehensive	493:505	arg1	resonance					524:532	A comprehensive nuclear magnetic resonance	491:532	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures	491:625	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	1	63	from	WCFS1	221:225	arg1	capacity					159:166	the high capacity	150:166	the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS)	150:488	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	1	63	from	WCFS1	221:225	arg1	α-galactosidase					176:190	MelA α-galactosidase	171:190	MelA α-galactosidase from Lactobacillus plantarum WCFS1	171:225	This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS).					
33434031	4	64	theme	in	1082:1083	arg1	assessment					1091:1100	An in vitro assessment	1079:1100	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS	1079:1164	An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose.					
33434031	2	65	theme	-α-d-galactopyranosyl-	879:900	arg1	-β-d-galactopyranosyl-					908:929	α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-	851:929	α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)	851:936	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
33434031	2	65	theme	-α-d-galactopyranosyl-	879:900	arg1	→					933:933	1 → 4	931:935	1 → 4	931:935	A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose as the main component as well as the presence of α-d-galactopyranosyl-(1 → 3)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose and α-d-galactopyranosyl-(1 → 6)-α-d-galactopyranosyl-(1 → 6)-β-d-galactopyranosyl-(1 → 4)-β-d-fructose.					
32375991	8	0	theme	most	1199:1202	arg1	isolates					1216:1223	most serotype 14 isolates	1199:1223	most serotype 14 isolates from South Africa	1199:1241	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	12	1	theme	GPSC23	1829:1834	arg1	phylogeny					1836:1844	the GPSC23 phylogeny	1825:1844	the GPSC23 phylogeny	1825:1844	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	1	2	theme	pneumococcal	280:291	arg1	disease					293:299	global pneumococcal disease	273:299	global pneumococcal disease	273:299	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	17	3	theme	accessible	2395:2404	arg1	assignment					2406:2415	The accessible assignment	2391:2415	The accessible assignment of GPSCs	2391:2424	The accessible assignment of GPSCs allows others to contextualize their own collections beyond the data presented here.					
32375991	3	4	theme	Global	600:605	arg1	GPSCs					639:643	GPSCs	639:643	GPSCs	639:643	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	3	4	theme	Global	600:605	arg1	Clusters					629:636	73 common Global Pneumococcal Sequence Clusters	590:636	73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK	590:677	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	16	5	used	used	2321:2324	arg2	resources					2287:2295	The resources	2283:2295	The resources we have provided	2283:2312	The resources we have provided can be used to explore to data, test hypothesis and generate new hypotheses.					
32375991	4	6	theme	Gene	712:715	arg1	presence					717:724	Gene presence	712:724	Gene presence	712:724	Gene presence and absence defined using Roary, and recombination profiles derived from Gubbins are presented in Phandango for each GPSC.					
32375991	1	7	theme	geographic	190:199	arg1	distribution					201:212	their geographic distribution	184:212	their geographic distribution	184:212	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	1	7	theme	geographic	190:199	arg1	lineages					174:181	pneumococcal lineages	161:181	pneumococcal lineages	161:181	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	12	8	theme	genes	1796:1800	arg1	presence/absence					1765:1780	gene presence/absence	1760:1780	gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny	1760:1844	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	11	9	dep	19A	1680:1682	arg1	estimated					1684:1692	estimated	1684:1692	19A estimated to have occured in the 1990s from the GPSC97-dated tree	1680:1748	This was consistent with a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree.					
32375991	0	10	theme	pneumococcal	125:136	arg1	isolates					138:145	pneumococcal isolates	125:145	pneumococcal isolates	125:145	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	3	11	theme	Sequence	620:627	arg1	GPSCs					639:643	GPSCs	639:643	GPSCs	639:643	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	3	11	theme	Sequence	620:627	arg1	Clusters					629:636	73 common Global Pneumococcal Sequence Clusters	590:636	73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK	590:677	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	15	12	theme	USA-specific	2194:2205	arg1	clade					2207:2211	a USA-specific clade	2192:2211	a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981	2192:2280	Most of the Utah isolates clustered within GPSC31 in a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981.					
32375991	8	13	theme	African	1337:1343	arg1	isolates					1357:1364	South African serotype 14 isolates	1331:1364	South African serotype 14 isolates	1331:1364	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	4	14	attach	derived	786:792	arg2	profiles					777:784	recombination profiles	763:784	recombination profiles derived from Gubbins	763:805	Gene presence and absence defined using Roary, and recombination profiles derived from Gubbins are presented in Phandango for each GPSC.					
32375991	4	14	attach	derived	786:792	arg1	Gubbins					799:805	Gubbins	799:805	Gubbins	799:805	Gene presence and absence defined using Roary, and recombination profiles derived from Gubbins are presented in Phandango for each GPSC.					
32375991	17	15	theme	GPSCs	2420:2424	arg1	assignment					2406:2415	The accessible assignment	2391:2415	The accessible assignment of GPSCs	2391:2424	The accessible assignment of GPSCs allows others to contextualize their own collections beyond the data presented here.					
32375991	7	16	theme	unrelated	1093:1101	arg1	backgrounds					1111:1121	nine unrelated genetic backgrounds	1088:1121	nine unrelated genetic backgrounds in South Africa	1088:1137	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	12	17	theme	presence/absence	1765:1780	arg1	Plots					1751:1755	Plots	1751:1755	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny	1751:1844	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	3	18	theme	common	593:598	arg1	GPSCs					639:643	GPSCs	639:643	GPSCs	639:643	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	3	18	theme	common	593:598	arg1	Clusters					629:636	73 common Global Pneumococcal Sequence Clusters	590:636	73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK	590:677	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	12	19	theme	resistance	1785:1794	arg1	genes					1796:1800	resistance genes	1785:1800	resistance genes (tet, erm, cat)	1785:1816	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	12	20	dep	genes	1796:1800	arg1	tet					1803:1805	tet	1803:1805	tet	1803:1805	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	12	20	dep	genes	1796:1800	arg1	cat					1813:1815	cat	1813:1815	cat	1813:1815	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	12	20	dep	genes	1796:1800	arg1	erm					1808:1810	erm	1808:1810	erm	1808:1810	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	7	21	theme	phylogenetic	1019:1030	arg1	snapshot					1032:1039	a country-specific phylogenetic snapshot	1000:1039	a country-specific phylogenetic snapshot	1000:1039	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	1	22	theme	pneumococcal	161:172	arg1	distribution					201:212	their geographic distribution	184:212	their geographic distribution	184:212	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	1	22	theme	pneumococcal	161:172	arg1	patterns					240:247	antibiotic resistance patterns	218:247	antibiotic resistance patterns	218:247	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	1	22	theme	pneumococcal	161:172	arg1	lineages					174:181	pneumococcal lineages	161:181	pneumococcal lineages	161:181	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	8	23	theme	serotype	1204:1211	arg1	isolates					1216:1223	most serotype 14 isolates	1199:1223	most serotype 14 isolates from South Africa	1199:1241	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	13	24	theme	GPSC23-dated	1928:1939	arg1	tree					1941:1944	the GPSC23-dated tree	1924:1944	the GPSC23-dated tree that the acquisition occurred between 1953 and 1975	1924:1996	We estimated from the GPSC23-dated tree that the acquisition occurred between 1953 and 1975.					
32375991	15	25	theme	common	2234:2239	arg1	ancestor					2241:2248	the most recent common ancestor	2218:2248	the most recent common ancestor estimated between 1958 and 1981	2218:2280	Most of the Utah isolates clustered within GPSC31 in a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981.					
32375991	11	26	from	tree	1745:1748	arg1	1990s					1717:1721	the 1990s	1713:1721	the 1990s from the GPSC97-dated tree	1713:1748	This was consistent with a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree.					
32375991	0	27	theme	population	87:96	arg1	snapshots					98:106	country population snapshots	79:106	country population snapshots	79:106	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	2	28	theme	interactive	313:323	arg1	outputs					339:345	interactive bioinformatic outputs	313:345	interactive bioinformatic outputs to explore such topics	313:368	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	9	29	dep	established	1438:1448	arg1	each					1433:1436	each	1433:1436	each	1433:1436	We estimated from the GPSC9-dated tree that the sub-clusters were each established in South Africa during the 1980s.					
32375991	2	30	theme	wider	431:435	arg1	community					437:445	the wider community	427:445	the wider community	427:445	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	6	31	used	used	972:975	arg2	resources					955:963	such resources	950:963	such resources	950:963	We provide examples of how such resources can be used.					
32375991	7	32	located	observed	1076:1083	arg1	backgrounds					1111:1121	nine unrelated genetic backgrounds	1088:1121	nine unrelated genetic backgrounds in South Africa	1088:1137	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	7	32	located	observed	1076:1083	arg2	serotype					1060:1067	serotype 14	1060:1070	serotype 14	1060:1070	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	9	33	theme	GPSC9-dated	1389:1399	arg1	tree					1401:1404	the GPSC9-dated tree	1385:1404	the GPSC9-dated tree that the sub-clusters were each established in South Africa during the 1980s	1385:1481	We estimated from the GPSC9-dated tree that the sub-clusters were each established in South Africa during the 1980s.					
32375991	11	34	with	consistent	1635:1644	arg1	switch					1653:1658	a switch	1651:1658	a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree	1651:1748	This was consistent with a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree.					
32375991	14	35	from	Utah	2115:2118	arg1	assemblies					2099:2108	17 externally generated pneumococcal serotype 1 assemblies	2051:2108	17 externally generated pneumococcal serotype 1 assemblies from Utah	2051:2118	Finally, we demonstrate the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah via Pathogenwatch.					
32375991	10	36	theme	20 kb	1548:1552	arg1	recombination					1554:1566	a 20 kb recombination	1546:1566	a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97	1546:1623	We show how recombination plots allowed the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97.					
32375991	0	37	theme	Pneumococcal	36:47	arg1	GPSCs					68:72	GPSCs	68:72	GPSCs	68:72	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	0	37	theme	Pneumococcal	36:47	arg1	Clusters					58:65	Global Pneumococcal Sequence Clusters	29:65	Global Pneumococcal Sequence Clusters (GPSCs)	29:73	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	15	38	with	clade	2207:2211	arg1	ancestor					2241:2248	the most recent common ancestor	2218:2248	the most recent common ancestor estimated between 1958 and 1981	2218:2280	Most of the Utah isolates clustered within GPSC31 in a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981.					
32375991	5	39	theme	Temporal	849:856	arg1	signal					871:876	Temporal phylogenetic signal	849:876	Temporal phylogenetic signal	849:876	Temporal phylogenetic signal was assessed for each GPSC using BactDating.					
32375991	8	40	theme	phylogenetic	1158:1169	arg1	snapshot					1171:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	17	41	theme	own	2463:2465	arg1	collections					2467:2477	their own collections	2457:2477	their own collections	2457:2477	The accessible assignment of GPSCs allows others to contextualize their own collections beyond the data presented here.					
32375991	10	42	theme	capsular	1581:1588	arg1	polysaccharide					1590:1603	the capsular polysaccharide	1577:1603	the capsular polysaccharide locus	1577:1609	We show how recombination plots allowed the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97.					
32375991	3	43	theme	country-specific	505:520	arg1	snapshots					535:543	12 country-specific phylogenetic snapshots	502:543	12 country-specific phylogenetic snapshots	502:543	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	12	44	theme	composite	1884:1892	arg1	transposon					1894:1903	a composite transposon	1882:1903	a composite transposon	1882:1903	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	3	45	theme	phylogenetic	564:575	arg1	snapshots					577:585	phylogenetic snapshots	564:585	phylogenetic snapshots	564:585	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	14	46	theme	serotype	2088:2095	arg1	assemblies					2099:2108	17 externally generated pneumococcal serotype 1 assemblies	2051:2108	17 externally generated pneumococcal serotype 1 assemblies from Utah	2051:2118	Finally, we demonstrate the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah via Pathogenwatch.					
32375991	1	47	theme	global	273:278	arg1	disease					293:299	global pneumococcal disease	273:299	global pneumococcal disease	273:299	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	8	48	from	Africa	1236:1241	arg1	isolates					1216:1223	most serotype 14 isolates	1199:1223	most serotype 14 isolates from South Africa	1199:1241	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	7	49	from	backgrounds	1111:1121	arg1	Africa					1132:1137	Africa	1132:1137	Africa	1132:1137	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	2	50	theme	bioinformatic	325:337	arg1	outputs					339:345	interactive bioinformatic outputs	313:345	interactive bioinformatic outputs to explore such topics	313:368	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	3	51	theme	Clusters	629:636	arg1	snapshots					535:543	12 country-specific phylogenetic snapshots	502:543	12 country-specific phylogenetic snapshots	502:543	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	6	52	theme	such	950:953	arg1	resources					955:963	such resources	950:963	such resources	950:963	We provide examples of how such resources can be used.					
32375991	15	53	theme	recent	2227:2232	arg1	ancestor					2241:2248	the most recent common ancestor	2218:2248	the most recent common ancestor estimated between 1958 and 1981	2218:2280	Most of the Utah isolates clustered within GPSC31 in a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981.					
32375991	11	54	theme	GPSC97-dated	1732:1743	arg1	tree					1745:1748	the GPSC97-dated tree	1728:1748	the GPSC97-dated tree	1728:1748	This was consistent with a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree.					
32375991	16	55	theme	new	2375:2377	arg1	hypotheses					2379:2388	new hypotheses	2375:2388	new hypotheses	2375:2388	The resources we have provided can be used to explore to data, test hypothesis and generate new hypotheses.					
32375991	2	56	theme	such	358:361	arg1	topics					363:368	such topics	358:368	such topics	358:368	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	12	57	with	consistent	1851:1860	arg1	acquisition					1867:1877	acquisition	1867:1877	acquisition of a composite transposon	1867:1903	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	8	58	theme	independent	1291:1301	arg1	sub-clusters					1303:1314	three independent sub-clusters	1285:1314	three independent sub-clusters represented by South African serotype 14 isolates	1285:1364	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	3	59	theme	Pneumococcal	607:618	arg1	GPSCs					639:643	GPSCs	639:643	GPSCs	639:643	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	3	59	theme	Pneumococcal	607:618	arg1	Clusters					629:636	73 common Global Pneumococcal Sequence Clusters	590:636	73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK	590:677	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	16	60	dep	resources	2287:2295	arg1	provided					2305:2312	provided	2305:2312	have provided	2300:2312	The resources we have provided can be used to explore to data, test hypothesis and generate new hypotheses.					
32375991	1	61	theme	antibiotic	218:227	arg1	lineages					174:181	pneumococcal lineages	161:181	pneumococcal lineages	161:181	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	1	61	theme	antibiotic	218:227	arg1	patterns					240:247	antibiotic resistance patterns	218:247	antibiotic resistance patterns	218:247	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	7	62	theme	genetic	1103:1109	arg1	backgrounds					1111:1121	nine unrelated genetic backgrounds	1088:1121	nine unrelated genetic backgrounds in South Africa	1088:1137	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	11	63	from	serotype	1665:1672	arg1	switch					1653:1658	a switch	1651:1658	a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree	1651:1748	This was consistent with a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree.					
32375991	8	64	theme	GPSC9	1183:1187	arg1	snapshot					1171:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	3	65	attach	present	684:690	arg2	We					490:491	We	490:491	We	490:491	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	3	65	attach	present	684:690	arg1	Microreact					700:709	Microreact	700:709	Microreact	700:709	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	8	66	theme	serotype	1345:1352	arg1	isolates					1357:1364	South African serotype 14 isolates	1331:1364	South African serotype 14 isolates	1331:1364	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	12	67	theme	gene	1760:1763	arg1	presence/absence					1765:1780	gene presence/absence	1760:1780	gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny	1760:1844	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	4	68	theme	recombination	763:775	arg1	profiles					777:784	recombination profiles	763:784	recombination profiles derived from Gubbins	763:805	Gene presence and absence defined using Roary, and recombination profiles derived from Gubbins are presented in Phandango for each GPSC.					
32375991	0	69	theme	country	79:85	arg1	snapshots					98:106	country population snapshots	79:106	country population snapshots	79:106	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	7	70	theme	snapshot	1032:1039	arg1	use					993:995	our example use	981:995	our example use of a country-specific phylogenetic snapshot	981:1039	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	1	71	theme	lineages	174:181	arg1	Knowledge					148:156	Knowledge	148:156	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns,	148:248	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	10	72	theme	recombination	1496:1508	arg1	plots					1510:1514	recombination plots	1496:1514	recombination plots	1496:1514	We show how recombination plots allowed the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97.					
32375991	7	73	theme	country-specific	1002:1017	arg1	snapshot					1032:1039	a country-specific phylogenetic snapshot	1000:1039	a country-specific phylogenetic snapshot	1000:1039	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	14	74	theme	generated	2065:2073	arg1	assemblies					2099:2108	17 externally generated pneumococcal serotype 1 assemblies	2051:2108	17 externally generated pneumococcal serotype 1 assemblies from Utah	2051:2118	Finally, we demonstrate the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah via Pathogenwatch.					
32375991	1	75	theme	resistance	229:238	arg1	lineages					174:181	pneumococcal lineages	161:181	pneumococcal lineages	161:181	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	1	75	theme	resistance	229:238	arg1	patterns					240:247	antibiotic resistance patterns	218:247	antibiotic resistance patterns	218:247	Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease.					
32375991	3	76	dep	snapshots	535:543	arg1	snapshots					577:585	phylogenetic snapshots	564:585	phylogenetic snapshots	564:585	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32375991	5	77	theme	phylogenetic	858:869	arg1	signal					871:876	Temporal phylogenetic signal	849:876	Temporal phylogenetic signal	849:876	Temporal phylogenetic signal was assessed for each GPSC using BactDating.					
32375991	7	78	theme	example	985:991	arg1	use					993:995	our example use	981:995	our example use of a country-specific phylogenetic snapshot	981:1039	In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa.					
32375991	14	79	theme	GPSC31	2041:2046	arg1	assignment					2027:2036	the assignment	2023:2036	the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah	2023:2118	Finally, we demonstrate the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah via Pathogenwatch.					
32375991	2	80	theme	specialist	469:478	arg1	training					480:487	specialist training	469:487	specialist training	469:487	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	2	81	theme	insights	415:422	arg1	dissemination					390:402	dissemination	390:402	dissemination of genomic insights to the wider community	390:445	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	0	82	theme	Global	29:34	arg1	GPSCs					68:72	GPSCs	68:72	GPSCs	68:72	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	0	82	theme	Global	29:34	arg1	Clusters					58:65	Global Pneumococcal Sequence Clusters	29:65	Global Pneumococcal Sequence Clusters (GPSCs)	29:73	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	10	83	theme	recombination	1554:1566	arg1	identification					1528:1541	the identification	1524:1541	the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97	1524:1623	We show how recombination plots allowed the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97.					
32375991	2	84	theme	genomic	407:413	arg1	insights					415:422	genomic insights	407:422	genomic insights	407:422	We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemination of genomic insights to the wider community, without the need for specialist training.					
32375991	0	85	theme	Sequence	49:56	arg1	GPSCs					68:72	GPSCs	68:72	GPSCs	68:72	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	0	85	theme	Sequence	49:56	arg1	Clusters					58:65	Global Pneumococcal Sequence Clusters	29:65	Global Pneumococcal Sequence Clusters (GPSCs)	29:73	Visualizing variation within Global Pneumococcal Sequence Clusters (GPSCs) and country population snapshots to contextualize pneumococcal isolates.					
32375991	8	86	theme	international	1144:1156	arg1	snapshot					1171:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	15	87	theme	Utah	2151:2154	arg1	isolates					2156:2163	the Utah isolates	2147:2163	the Utah isolates	2147:2163	Most of the Utah isolates clustered within GPSC31 in a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981.					
32375991	10	88	theme	polysaccharide	1590:1603	arg1	locus					1605:1609	the capsular polysaccharide locus	1577:1609	the capsular polysaccharide locus	1577:1609	We show how recombination plots allowed the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97.					
32375991	8	89	located	observed	1248:1255	arg1	snapshot					1171:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot	1140:1178	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	8	89	located	observed	1248:1255	arg2	isolates					1216:1223	most serotype 14 isolates	1199:1223	most serotype 14 isolates from South Africa	1199:1241	The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates.					
32375991	12	90	theme	transposon	1894:1903	arg1	acquisition					1867:1877	acquisition	1867:1877	acquisition of a composite transposon	1867:1903	Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon.					
32375991	14	91	theme	pneumococcal	2075:2086	arg1	assemblies					2099:2108	17 externally generated pneumococcal serotype 1 assemblies	2051:2108	17 externally generated pneumococcal serotype 1 assemblies from Utah	2051:2118	Finally, we demonstrate the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah via Pathogenwatch.					
32375991	3	92	theme	phylogenetic	522:533	arg1	snapshots					535:543	12 country-specific phylogenetic snapshots	502:543	12 country-specific phylogenetic snapshots	502:543	We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact.					
32478918	4	0	theme	disc	912:915	arg1	volume					917:922	disc volume	912:922	disc volume	912:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	9	1	theme	resting	1942:1948	arg1	shape					1950:1954	the disc resting shape	1933:1954	the disc resting shape	1933:1954	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	4	2	theme	joint	645:649	arg1	discs					651:655	porcine temporomandibular joint discs	619:655	porcine temporomandibular joint discs	619:655	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	2	3	theme	temporomandibular	362:378	arg1	biomechanics					391:402	temporomandibular joint disc biomechanics	362:402	temporomandibular joint disc biomechanics	362:402	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	6	4	theme	elastase	1457:1464	arg1	activity					1445:1452	non-specific activity	1432:1452	non-specific activity of elastase	1432:1464	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	4	5	dep	test	1035:1038	arg1	d					1013:1013	d	1013:1013	d	1013:1013	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	7	6	theme	elastin	1482:1488	arg1	fibers					1490:1495	elastin fibers	1482:1495	elastin fibers	1482:1495	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	6	7	theme	elastin	1323:1329	arg1	depletion					1310:1318	significant depletion	1298:1318	significant depletion of elastin (50%)	1298:1335	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	4	8	theme	cell	896:899	arg1	shape					901:905	cell shape	896:905	cell shape	896:905	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	2	9	from	contribution	346:357	arg1	biomechanics					391:402	temporomandibular joint disc biomechanics	362:402	temporomandibular joint disc biomechanics	362:402	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	9	10	dep	restoration	1902:1912	arg1	the					1898:1900	the	1898:1900	the	1898:1900	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	2	11	theme	elastin	277:283	arg1	fibers					285:290	elastin fibers	277:290	elastin fibers	277:290	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	9	12	theme	collagen	1983:1990	arg1	fibers					1992:1997	collagen fibers	1983:1997	collagen fibers	1983:1997	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	2	13	theme	connective	322:331	arg1	tissues					333:339	connective tissues	322:339	connective tissues	322:339	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	1	14	theme	elastin	214:220	arg1	fibers					222:227	elastin fibers	214:227	elastin fibers	214:227	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	7	15	theme	cell	1660:1663	arg1	elongation					1665:1674	cell elongation	1660:1674	cell elongation	1660:1674	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	8	16	theme	maximal	1729:1735	arg1	dissipation					1744:1754	the maximal energy dissipation	1725:1754	the maximal energy dissipation across the intermediate regions	1725:1786	Mechanically, elastase treatment nearly doubled the maximal energy dissipation across the intermediate regions while the instantaneous modulus was not significantly affected.					
32478918	6	17	theme	regions	1283:1289	arg1	analyses					1254:1261	biochemical analyses	1242:1261	biochemical analyses of the intermediate regions	1242:1289	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	9	18	theme	mechanical	2010:2019	arg1	resilience					2021:2030	mechanical resilience	2010:2030	mechanical resilience to the temporomandibular joint disc	2010:2066	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	4	19	theme	mechanical	709:718	arg1	changes					729:735	mechanical regional changes	709:735	mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test	709:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	5	20	theme	elastin	1121:1127	arg1	fibers					1129:1134	elastin fibers	1121:1134	elastin fibers	1121:1134	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	4	21	dep	analysis	863:870	arg1	b					847:847	b	847:847	b	847:847	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	7	22	theme	tortuosity	1645:1654	arg1	reduction					1623:1631	remarkable reduction	1612:1631	remarkable reduction of collagen tortuosity and cell elongation	1612:1674	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	6	23	from	decrease	1354:1361	arg1	%					1378:1378	20%	1376:1378	20%	1376:1378	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	6	23	from	decrease	1354:1361	arg1	collagen					1366:1373	collagen (20%) and glycosaminoglycan (49%) content	1366:1415	collagen	1366:1373	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	6	23	from	decrease	1354:1361	arg1	content					1409:1415	collagen (20%) and glycosaminoglycan (49%) content	1366:1415	content	1409:1415	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	6	23	from	decrease	1354:1361	arg1	%					1406:1406	49%	1404:1406	49%	1404:1406	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	4	24	theme	collagen	762:769	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	24	theme	collagen	762:769	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	24	theme	collagen	762:769	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	24	theme	collagen	762:769	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	25	dep	structural	678:687	arg1	the					674:676	the	674:676	the	674:676	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	1	26	theme	joint	139:143	arg1	disc					145:148	The temporomandibular joint disc	117:148	The temporomandibular joint disc	117:148	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	1	26	theme	joint	139:143	arg1	structure					174:182	a fibrocartilaginous structure	153:182	a fibrocartilaginous structure	153:182	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	4	27	theme	biochemical	929:939	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	9	28	theme	temporomandibular	2039:2055	arg1	disc					2063:2066	the temporomandibular joint disc	2035:2066	the temporomandibular joint disc	2035:2066	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	4	29	theme	elastin	775:781	arg1	fibers					783:788	elastin fibers	775:788	elastin fibers	775:788	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	7	30	theme	remarkable	1612:1621	arg1	reduction					1623:1631	remarkable reduction	1612:1631	remarkable reduction of collagen tortuosity and cell elongation	1612:1674	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	0	31	theme	temporomandibular	58:74	arg1	disc					82:85	the temporomandibular joint disc	54:85	the temporomandibular joint disc	54:85	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	1	32	theme	fibrocartilaginous	155:172	arg1	disc					145:148	The temporomandibular joint disc	117:148	The temporomandibular joint disc	117:148	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	1	32	theme	fibrocartilaginous	155:172	arg1	structure					174:182	a fibrocartilaginous structure	153:182	a fibrocartilaginous structure	153:182	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	0	33	theme	disc	82:85	arg1	properties					40:49	structural and mechanical properties	14:49	structural and mechanical properties of the temporomandibular joint disc	14:85	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	7	34	theme	volume	1578:1583	arg1	enlargement					1585:1595	its significant volume enlargement	1562:1595	its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation	1562:1674	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	4	35	theme	electron	825:832	arg1	microscopy					834:843	transmission electron microscopy	812:843	transmission electron microscopy	812:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	0	36	theme	elastase	97:104	arg1	digestion					106:114	elastase digestion	97:114	elastase digestion	97:114	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	4	37	theme	content	999:1005	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	37	theme	content	999:1005	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	37	theme	content	999:1005	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	37	theme	content	999:1005	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	3	38	theme	structural-functional	466:486	arg1	contribution					488:499	the structural-functional contribution	462:499	the structural-functional contribution of elastin in the temporomandibular joint disc	462:546	This study attempts to characterize the structural-functional contribution of elastin in the temporomandibular joint disc.					
32478918	4	39	dep	quantification	941:954	arg1	c					926:926	c	926:926	c	926:926	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	5	40	theme	temporomandibular	1147:1163	arg1	disc					1171:1174	the temporomandibular joint disc	1143:1174	the temporomandibular joint disc	1143:1174	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	6	41	theme	non-specific	1432:1443	arg1	activity					1445:1452	non-specific activity	1432:1452	non-specific activity of elastase	1432:1464	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	9	42	theme	elastin	1869:1875	arg1	fibers					1877:1882	elastin fibers	1869:1882	elastin fibers	1869:1882	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	4	43	theme	fiber	602:606	arg1	network					608:614	the elastin fiber network	590:614	the elastin fiber network in porcine temporomandibular joint discs	590:655	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	0	44	theme	structural	14:23	arg1	properties					40:49	structural and mechanical properties	14:49	structural and mechanical properties of the temporomandibular joint disc	14:85	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	4	45	theme	cyclic	1016:1021	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	0	46	theme	mechanical	29:38	arg1	properties					40:49	structural and mechanical properties	14:49	structural and mechanical properties of the temporomandibular joint disc	14:85	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	4	47	theme	tortuosity	884:893	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	47	theme	tortuosity	884:893	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	47	theme	tortuosity	884:893	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	47	theme	tortuosity	884:893	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	3	48	theme	temporomandibular	519:535	arg1	disc					543:546	the temporomandibular joint disc	515:546	the temporomandibular joint disc	515:546	This study attempts to characterize the structural-functional contribution of elastin in the temporomandibular joint disc.					
32478918	4	49	theme	quantitative	850:861	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	8	50	theme	elastase	1691:1698	arg1	treatment					1700:1708	elastase treatment	1691:1708	elastase treatment	1691:1708	Mechanically, elastase treatment nearly doubled the maximal energy dissipation across the intermediate regions while the instantaneous modulus was not significantly affected.					
32478918	7	51	theme	fibers	1490:1495	arg1	Degradation					1467:1477	Degradation	1467:1477	Degradation of elastin fibers	1467:1495	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	9	52	theme	disc	1937:1940	arg1	shape					1950:1954	the disc resting shape	1933:1954	the disc resting shape	1933:1954	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	5	53	from	effect	1200:1205	arg1	regions					1227:1233	the intermediate regions	1210:1233	the intermediate regions	1210:1233	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	3	54	from	contribution	488:499	arg1	disc					543:546	the temporomandibular joint disc	515:546	the temporomandibular joint disc	515:546	This study attempts to characterize the structural-functional contribution of elastin in the temporomandibular joint disc.					
32478918	2	55	theme	joint	380:384	arg1	biomechanics					391:402	temporomandibular joint disc biomechanics	362:402	temporomandibular joint disc biomechanics	362:402	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	9	56	theme	shape	1950:1954	arg1	maintenance					1918:1928	maintenance	1918:1928	maintenance	1918:1928	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	9	56	theme	shape	1950:1954	arg1	restoration					1902:1912	restoration	1902:1912	restoration	1902:1912	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	4	57	theme	temporomandibular	627:643	arg1	discs					651:655	porcine temporomandibular joint discs	619:655	porcine temporomandibular joint discs	619:655	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	2	58	theme	load-bearing	295:306	arg1	properties					308:317	load-bearing properties	295:317	load-bearing properties of connective tissues	295:339	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	4	59	theme	volume	917:922	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	59	theme	volume	917:922	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	59	theme	volume	917:922	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	59	theme	volume	917:922	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	2	60	theme	fibers	285:290	arg1	role					269:272	the crucial role	257:272	the crucial role of elastin fibers in load-bearing properties of connective tissues	257:339	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	8	61	theme	energy	1737:1742	arg1	dissipation					1744:1754	the maximal energy dissipation	1725:1754	the maximal energy dissipation across the intermediate regions	1725:1786	Mechanically, elastase treatment nearly doubled the maximal energy dissipation across the intermediate regions while the instantaneous modulus was not significantly affected.					
32478918	4	62	theme	regional	720:727	arg1	changes					729:735	mechanical regional changes	709:735	mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test	709:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	6	63	theme	glycosaminoglycan	1385:1401	arg1	content					1409:1415	collagen (20%) and glycosaminoglycan (49%) content	1366:1415	content	1409:1415	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	6	63	theme	glycosaminoglycan	1385:1401	arg1	%					1406:1406	49%	1404:1406	49%	1404:1406	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	6	64	theme	significant	1298:1308	arg1	depletion					1310:1318	significant depletion	1298:1318	significant depletion of elastin (50%)	1298:1335	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	2	65	theme	tissues	333:339	arg1	properties					308:317	load-bearing properties	295:317	load-bearing properties of connective tissues	295:339	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	1	66	theme	collagen	197:204	arg1	fibers					206:211	collagen fibers	197:211	collagen fibers	197:211	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	7	67	theme	elongation	1665:1674	arg1	reduction					1623:1631	remarkable reduction	1612:1631	remarkable reduction of collagen tortuosity and cell elongation	1612:1674	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	4	68	theme	shape	901:905	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	68	theme	shape	901:905	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	68	theme	shape	901:905	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	68	theme	shape	901:905	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	8	69	theme	intermediate	1767:1778	arg1	regions					1780:1786	the intermediate regions	1763:1786	the intermediate regions	1763:1786	Mechanically, elastase treatment nearly doubled the maximal energy dissipation across the intermediate regions while the instantaneous modulus was not significantly affected.					
32478918	7	70	theme	collagen	1636:1643	arg1	tortuosity					1645:1654	collagen tortuosity	1636:1654	collagen tortuosity	1636:1654	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	4	71	theme	collagen	959:966	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	71	theme	collagen	959:966	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	71	theme	collagen	959:966	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	71	theme	collagen	959:966	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	6	72	theme	intermediate	1270:1281	arg1	regions					1283:1289	the intermediate regions	1266:1289	the intermediate regions	1266:1289	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	6	73	theme	substantial	1342:1352	arg1	decrease					1354:1361	substantial decrease	1342:1361	substantial decrease in collagen (20%) and glycosaminoglycan (49%) content	1342:1415	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	5	74	theme	fibers	1129:1134	arg1	fragmentation					1104:1116	fragmentation	1104:1116	fragmentation of elastin fibers across the temporomandibular joint disc	1104:1174	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	8	75	theme	instantaneous	1798:1810	arg1	modulus					1812:1818	the instantaneous modulus	1794:1818	the instantaneous modulus	1794:1818	Mechanically, elastase treatment nearly doubled the maximal energy dissipation across the intermediate regions while the instantaneous modulus was not significantly affected.					
32478918	1	76	theme	temporomandibular	121:137	arg1	disc					145:148	The temporomandibular joint disc	117:148	The temporomandibular joint disc	117:148	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	1	76	theme	temporomandibular	121:137	arg1	structure					174:182	a fibrocartilaginous structure	153:182	a fibrocartilaginous structure	153:182	The temporomandibular joint disc is a fibrocartilaginous structure, composed of collagen fibers, elastin fibers, and proteoglycans.					
32478918	5	77	theme	microscopic	1071:1081	arg1	examination					1083:1093	microscopic examination	1071:1093	microscopic examination	1071:1093	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	6	78	theme	biochemical	1242:1252	arg1	analyses					1254:1261	biochemical analyses	1242:1261	biochemical analyses of the intermediate regions	1242:1289	Also, biochemical analyses of the intermediate regions showed significant depletion of elastin (50%), and substantial decrease in collagen (20%) and glycosaminoglycan (49%) content, likely due to non-specific activity of elastase.					
32478918	9	79	theme	joint	2057:2061	arg1	disc					2063:2066	the temporomandibular joint disc	2035:2066	the temporomandibular joint disc	2035:2066	We conclude that elastin fibers contribute to the restoration and maintenance of the disc resting shape and actively interact with collagen fibers to provide mechanical resilience to the temporomandibular joint disc.					
32478918	0	80	theme	joint	76:80	arg1	disc					82:85	the temporomandibular joint disc	54:85	the temporomandibular joint disc	54:85	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	2	81	theme	crucial	261:267	arg1	role					269:272	the crucial role	257:272	the crucial role of elastin fibers in load-bearing properties of connective tissues	257:339	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	7	82	theme	significant	1566:1576	arg1	enlargement					1585:1595	its significant volume enlargement	1562:1595	its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation	1562:1674	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	4	83	dep	analysis	750:757	arg1	a					747:747	a	747:747	a	747:747	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	84	theme	glycosaminoglycan	969:985	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	84	theme	glycosaminoglycan	969:985	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	84	theme	glycosaminoglycan	969:985	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	84	theme	glycosaminoglycan	969:985	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	85	theme	transmission	812:823	arg1	microscopy					834:843	transmission electron microscopy	812:843	transmission electron microscopy	812:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	86	theme	elastin	991:997	arg1	content					999:1005	elastin content	991:1005	elastin content	991:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	5	87	theme	joint	1165:1169	arg1	disc					1171:1174	the temporomandibular joint disc	1143:1174	the temporomandibular joint disc	1143:1174	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	4	88	theme	fibers	783:788	arg1	analysis					750:757	(a) analysis	746:757	(a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy	746:843	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	88	theme	fibers	783:788	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	88	theme	fibers	783:788	arg1	analysis					863:870	(b) quantitative analysis	846:870	(b) quantitative analysis of collagen tortuosity, cell shape, and disc volume	846:922	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	88	theme	fibers	783:788	arg1	quantification					941:954	(c) biochemical quantification	925:954	(c) biochemical quantification of collagen, glycosaminoglycan and elastin content	925:1005	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	89	from	network	608:614	arg1	discs					651:655	porcine temporomandibular joint discs	619:655	porcine temporomandibular joint discs	619:655	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	5	90	theme	intermediate	1214:1225	arg1	regions					1227:1233	the intermediate regions	1210:1233	the intermediate regions	1210:1233	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	4	91	theme	porcine	619:625	arg1	discs					651:655	porcine temporomandibular joint discs	619:655	porcine temporomandibular joint discs	619:655	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	0	92	theme	properties	40:49	arg1	Alteration					0:9	Alteration	0:9	Alteration of structural and mechanical properties of the temporomandibular joint disc	0:85	Alteration of structural and mechanical properties of the temporomandibular joint disc following elastase digestion.					
32478918	2	93	from	role	269:272	arg1	properties					308:317	load-bearing properties	295:317	load-bearing properties of connective tissues	295:339	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
32478918	3	94	theme	elastin	504:510	arg1	contribution					488:499	the structural-functional contribution	462:499	the structural-functional contribution of elastin in the temporomandibular joint disc	462:546	This study attempts to characterize the structural-functional contribution of elastin in the temporomandibular joint disc.					
32478918	5	95	theme	pronounced	1189:1198	arg1	effect					1200:1205	a more pronounced effect	1182:1205	a more pronounced effect in the intermediate regions	1182:1233	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	7	96	theme	disc	1543:1546	arg1	configuration					1522:1534	the homeostatic configuration	1506:1534	the homeostatic configuration	1506:1534	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	5	97	theme	elastase	1051:1058	arg1	treatment					1060:1068	elastase treatment	1051:1068	elastase treatment	1051:1068	Following elastase treatment, microscopic examination revealed fragmentation of elastin fibers across the temporomandibular joint disc, with a more pronounced effect in the intermediate regions.					
32478918	4	98	theme	compression	1023:1033	arg1	test					1035:1038	(d) cyclic compression test	1012:1038	(d) cyclic compression test	1012:1038	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	99	theme	elastin	594:600	arg1	network					608:614	the elastin fiber network	590:614	the elastin fiber network in porcine temporomandibular joint discs	590:655	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	4	100	theme	collagen	875:882	arg1	tortuosity					884:893	collagen tortuosity	875:893	collagen tortuosity	875:893	Using elastase, we selectively perturbed the elastin fiber network in porcine temporomandibular joint discs and investigated the structural, compositional, and mechanical regional changes through: (a) analysis of collagen and elastin fibers by immunolabeling and transmission electron microscopy; (b) quantitative analysis of collagen tortuosity, cell shape, and disc volume; (c) biochemical quantification of collagen, glycosaminoglycan and elastin content; and (d) cyclic compression test.					
32478918	3	101	theme	joint	537:541	arg1	disc					543:546	the temporomandibular joint disc	515:546	the temporomandibular joint disc	515:546	This study attempts to characterize the structural-functional contribution of elastin in the temporomandibular joint disc.					
32478918	7	102	theme	homeostatic	1510:1520	arg1	configuration					1522:1534	the homeostatic configuration	1506:1534	the homeostatic configuration	1506:1534	Degradation of elastin fibers affected the homeostatic configuration of the disc, reflected in its significant volume enlargement accompanied by remarkable reduction of collagen tortuosity and cell elongation.					
32478918	2	103	theme	disc	386:389	arg1	biomechanics					391:402	temporomandibular joint disc biomechanics	362:402	temporomandibular joint disc biomechanics	362:402	Despite the crucial role of elastin fibers in load-bearing properties of connective tissues, its contribution in temporomandibular joint disc biomechanics has been disregarded.					
33523381	4	0	theme	compounds	1015:1023	arg1	peroxidase					975:984	manganese peroxidase	965:984	manganese peroxidase	965:984	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	0	theme	compounds	1015:1023	arg1	phase					948:952	the mature phase	937:952	the mature phase	937:952	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	0	theme	compounds	1015:1023	arg1	xylanase					955:962	xylanase	955:962	xylanase	955:962	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	0	theme	compounds	1015:1023	arg1	utilization					991:1001	utilization	991:1001	utilization	991:1001	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	6	1	theme	functional	1388:1397	arg1	groups					1399:1404	the main functional groups	1379:1404	the main functional groups	1379:1404	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	3	2	from	effect	463:468	arg1	community					562:570	fungal community	555:570	fungal community	555:570	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	3	2	from	effect	463:468	arg1	degradation					519:529	the lignocellulose degradation	500:529	the lignocellulose degradation	500:529	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	3	2	from	effect	463:468	arg1	activities					539:548	enzyme activities	532:548	enzyme activities	532:548	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	1	3	theme	important	160:168	arg1	way					170:172	the most important way	151:172	the most important way to recycle medicinal herbal residues (MHRs)	151:216	Composting has become the most important way to recycle medicinal herbal residues (MHRs).					
33523381	6	4	theme	MHRs	1448:1451	arg1	process					1464:1470	the MHRs composting process	1444:1470	the MHRs composting process	1444:1470	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	0	5	theme	medicinal	91:99	arg1	residues					108:115	medicinal herbal residues	91:115	medicinal herbal residues composting	91:126	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	0	6	from	Effects	0:6	arg1	community					49:57	the fungal community	38:57	the fungal community	38:57	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	3	7	theme	multi-phase	473:483	arg1	inoculation					485:495	multi-phase inoculation	473:495	multi-phase inoculation	473:495	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	3	8	theme	lignocellulose	504:517	arg1	degradation					519:529	the lignocellulose degradation	500:529	the lignocellulose degradation	500:529	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	6	9	theme	multi-phase	1189:1199	arg1	treatment					1213:1221	multi-phase inoculation treatment	1189:1221	multi-phase inoculation treatment	1189:1221	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	0	10	theme	residues	108:115	arg1	improvement					76:86	the improvement	72:86	the improvement of medicinal herbal residues composting	72:126	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	2	11	theme	microbial	263:271	arg1	agent					273:277	a microbial agent	261:277	a microbial agent	261:277	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	4	12	theme	humic	791:795	arg1	acid					797:800	humic acid	791:800	humic acid	791:800	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	0	13	theme	herbal	101:106	arg1	residues					108:115	medicinal herbal residues	91:115	medicinal herbal residues composting	91:126	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	4	14	theme	humic	807:811	arg1	substances					813:822	humic substances	807:822	humic substances	807:822	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	2	15	theme	mutual	355:360	arg1	influence					362:370	mutual influence	355:370	mutual influence of microbial agents	355:390	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	5	16	theme	composting	1096:1105	arg1	process					1107:1113	the composting process	1092:1113	the composting process	1092:1113	Furthermore, the non-metric multi-dimensional scaling showed that the composting process and inoculation significantly influenced fungal community composition.					
33523381	6	17	theme	mature	1269:1274	arg1	phase					1276:1280	mesophilic, thermophilic, and mature phase	1239:1280	mesophilic, thermophilic, and mature phase	1239:1280	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	6	18	theme	thermophilic	1251:1262	arg1	phase					1276:1280	mesophilic, thermophilic, and mature phase	1239:1280	mesophilic, thermophilic, and mature phase	1239:1280	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	2	19	theme	compost	406:412	arg1	products					414:421	unstable compost products	397:421	unstable compost products	397:421	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	1	20	theme	medicinal	185:193	arg1	residues					202:209	medicinal herbal residues	185:209	medicinal herbal residues (MHRs)	185:216	Composting has become the most important way to recycle medicinal herbal residues (MHRs).					
33523381	1	20	theme	medicinal	185:193	arg1	MHRs					212:215	MHRs	212:215	MHRs	212:215	Composting has become the most important way to recycle medicinal herbal residues (MHRs).					
33523381	0	21	theme	inoculation	23:33	arg1	Effects					0:6	Effects	0:6	Effects of multi-phase inoculation on the fungal community	0:57	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	6	22	theme	mesophilic	1239:1248	arg1	phase					1276:1280	mesophilic, thermophilic, and mature phase	1239:1280	mesophilic, thermophilic, and mature phase	1239:1280	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	1	23	theme	herbal	195:200	arg1	residues					202:209	medicinal herbal residues	185:209	medicinal herbal residues (MHRs)	185:216	Composting has become the most important way to recycle medicinal herbal residues (MHRs).					
33523381	1	23	theme	herbal	195:200	arg1	MHRs					212:215	MHRs	212:215	MHRs	212:215	Composting has become the most important way to recycle medicinal herbal residues (MHRs).					
33523381	0	24	theme	multi-phase	11:21	arg1	inoculation					23:33	multi-phase inoculation	11:33	multi-phase inoculation	11:33	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	6	25	theme	inoculation	1201:1211	arg1	treatment					1213:1221	multi-phase inoculation treatment	1189:1221	multi-phase inoculation treatment	1189:1221	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	3	26	theme	enzyme	532:537	arg1	activities					539:548	enzyme activities	532:548	enzyme activities	532:548	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	2	27	theme	agents	385:390	arg1	products					414:421	unstable compost products	397:421	unstable compost products	397:421	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	2	27	theme	agents	385:390	arg1	efficiency					343:352	its low composting efficiency	324:352	its low composting efficiency	324:352	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	2	27	theme	agents	385:390	arg1	influence					362:370	mutual influence	355:370	mutual influence of microbial agents	355:390	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	4	28	theme	manganese	965:973	arg1	peroxidase					975:984	manganese peroxidase	965:984	manganese peroxidase	965:984	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	6	29	from	genus	1359:1363	arg1	treatment					1213:1221	multi-phase inoculation treatment	1189:1221	multi-phase inoculation treatment	1189:1221	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	6	30	theme	dominant	1350:1357	arg1	Thermomyces					1224:1234	Thermomyces	1224:1234	Thermomyces	1224:1234	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	6	30	theme	dominant	1350:1357	arg1	genus					1359:1363	the dominant genus	1346:1363	the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process	1346:1470	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	6	30	theme	dominant	1350:1357	arg1	unclassified_Sordariales					1283:1306	unclassified_Sordariales	1283:1306	unclassified_Sordariales	1283:1306	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	6	30	theme	dominant	1350:1357	arg1	Coprinopsis					1313:1323	Coprinopsis	1313:1323	Coprinopsis	1313:1323	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	4	31	theme	multi-phase	620:630	arg1	treatment					644:652	multi-phase inoculation treatment	620:652	multi-phase inoculation treatment	620:652	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	2	32	theme	traditional	223:233	arg1	method					246:251	The traditional composting method	219:251	The traditional composting method	219:251	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	4	33	theme	phenolic	1006:1013	arg1	compounds					1015:1023	phenolic compounds	1006:1023	phenolic compounds	1006:1023	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	2	34	theme	microbial	375:383	arg1	agents					385:390	microbial agents	375:390	microbial agents	375:390	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	4	35	theme	thermophilic	670:681	arg1	temperature					683:693	the highest thermophilic temperature	658:693	the highest thermophilic temperature (68.2 °C)	658:703	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	35	theme	thermophilic	670:681	arg1	68.2 °C					696:702	68.2 °C	696:702	68.2 °C	696:702	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	5	36	theme	multi-dimensional	1054:1070	arg1	scaling					1072:1078	the non-metric multi-dimensional scaling	1039:1078	the non-metric multi-dimensional scaling	1039:1078	Furthermore, the non-metric multi-dimensional scaling showed that the composting process and inoculation significantly influenced fungal community composition.					
33523381	2	37	theme	unstable	397:404	arg1	products					414:421	unstable compost products	397:421	unstable compost products	397:421	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	4	38	theme	cellulase	924:932	arg1	activities					910:919	the activities	906:919	the activities of cellulase	906:932	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	6	39	theme	main	1383:1386	arg1	groups					1399:1404	the main functional groups	1379:1404	the main functional groups	1379:1404	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	0	40	theme	fungal	42:47	arg1	community					49:57	the fungal community	38:57	the fungal community	38:57	Effects of multi-phase inoculation on the fungal community related with the improvement of medicinal herbal residues composting.					
33523381	5	41	theme	community	1163:1171	arg1	composition					1173:1183	fungal community composition	1156:1183	fungal community composition	1156:1183	Furthermore, the non-metric multi-dimensional scaling showed that the composting process and inoculation significantly influenced fungal community composition.					
33523381	4	42	theme	cellulose	870:878	arg1	degradation					855:865	the degradation	851:865	the degradation of cellulose and lignin	851:889	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	43	contain	had	654:656	arg1	treatment					644:652	multi-phase inoculation treatment	620:652	multi-phase inoculation treatment	620:652	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	43	contain	had	654:656	arg2	temperature					683:693	the highest thermophilic temperature	658:693	the highest thermophilic temperature (68.2 °C)	658:703	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	43	contain	had	654:656	arg2	%					734:734	102.68%	728:734	102.68%	728:734	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	43	contain	had	654:656	arg2	68.2 °C					696:702	68.2 °C	696:702	68.2 °C	696:702	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	43	contain	had	654:656	arg2	index					721:725	germination index	709:725	germination index (102.68%)	709:735	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	3	44	theme	fungal	555:560	arg1	community					562:570	fungal community	555:570	fungal community	555:570	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	6	45	theme	mature	1328:1333	arg1	phase					1335:1339	mature phase	1328:1339	mature phase	1328:1339	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	4	46	theme	phosphorus	771:780	arg1	content					782:788	available phosphorus content	761:788	available phosphorus content	761:788	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	47	theme	highest	662:668	arg1	temperature					683:693	the highest thermophilic temperature	658:693	the highest thermophilic temperature (68.2 °C)	658:703	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	47	theme	highest	662:668	arg1	68.2 °C					696:702	68.2 °C	696:702	68.2 °C	696:702	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	48	theme	germination	709:719	arg1	index					721:725	germination index	709:725	germination index (102.68%)	709:735	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	48	theme	germination	709:719	arg1	%					734:734	102.68%	728:734	102.68%	728:734	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	49	theme	lignin	884:889	arg1	degradation					855:865	the degradation	851:865	the degradation of cellulose and lignin	851:889	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	50	theme	available	761:769	arg1	content					782:788	available phosphorus content	761:788	available phosphorus content	761:788	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	5	51	theme	non-metric	1043:1052	arg1	scaling					1072:1078	the non-metric multi-dimensional scaling	1039:1078	the non-metric multi-dimensional scaling	1039:1078	Furthermore, the non-metric multi-dimensional scaling showed that the composting process and inoculation significantly influenced fungal community composition.					
33523381	3	52	theme	inoculation	485:495	arg1	effect					463:468	the effect	459:468	the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community	459:570	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	6	53	theme	composting	1453:1462	arg1	process					1464:1470	the MHRs composting process	1444:1470	the MHRs composting process	1444:1470	In multi-phase inoculation treatment, Thermomyces in mesophilic, thermophilic, and mature phase, unclassified_Sordariales, and Coprinopsis in mature phase were the dominant genus that might be the main functional groups to degrade lignocellulose and improve the MHRs composting process.					
33523381	3	54	theme	MHRs	579:582	arg1	composting					584:593	MHRs composting	579:593	MHRs composting	579:593	This study was conducted to assess the effect of multi-phase inoculation on the lignocellulose degradation, enzyme activities, and fungal community during MHRs composting.					
33523381	4	55	dep	content	782:788	arg1	concentration					824:836	concentration	824:836	concentration	824:836	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	2	56	theme	composting	332:341	arg1	efficiency					343:352	its low composting efficiency	324:352	its low composting efficiency	324:352	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	4	57	theme	inoculation	632:642	arg1	treatment					644:652	multi-phase inoculation treatment	620:652	multi-phase inoculation treatment	620:652	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	4	58	theme	mature	941:946	arg1	phase					948:952	the mature phase	937:952	the mature phase	937:952	The results showed that multi-phase inoculation treatment had the highest thermophilic temperature (68.2 °C) and germination index (102.68%), significantly improved available phosphorus content, humic acid, and humic substances concentration, accelerated the degradation of cellulose and lignin, and increased the activities of cellulase in the mature phase, xylanase, manganese peroxidase, and utilization of phenolic compounds.					
33523381	2	59	theme	composting	235:244	arg1	method					246:251	The traditional composting method	219:251	The traditional composting method	219:251	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	2	60	theme	low	328:330	arg1	efficiency					343:352	its low composting efficiency	324:352	its low composting efficiency	324:352	The traditional composting method, adding a microbial agent at one time, has been greatly limited due to its low composting efficiency, mutual influence of microbial agents, and unstable compost products.					
33523381	5	61	theme	fungal	1156:1161	arg1	composition					1173:1183	fungal community composition	1156:1183	fungal community composition	1156:1183	Furthermore, the non-metric multi-dimensional scaling showed that the composting process and inoculation significantly influenced fungal community composition.					
33783000	9	0	theme	layer	949:953	arg1	chromatography					955:968	A thin layer chromatography	942:968	A thin layer chromatography analysis	942:977	A thin layer chromatography analysis revealed glucose as the enzyme's main starch hydrolysis product.					
33783000	1	1	theme	Aspergillus	229:239	arg1	section					254:260	the Aspergillus genus Cremei section	225:260	the Aspergillus genus Cremei section	225:260	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	11	2	theme	GH15	1273:1276	arg1	domain					1279:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	10	3	used	used	1111:1114	arg2	sequence					1064:1071	An enzyme's peptide sequence	1044:1071	An enzyme's peptide sequence	1044:1071	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	5	4	theme	optimal	621:627	arg1	5.0					660:662	5.0	660:662	5.0	660:662	The optimal enzyme pH and temperature were 5.0 and 60°C (at pH 5.0), respectively.					
33783000	5	4	theme	optimal	621:627	arg1	pH					636:637	The optimal enzyme pH	617:637	The optimal enzyme pH	617:637	The optimal enzyme pH and temperature were 5.0 and 60°C (at pH 5.0), respectively.					
33783000	8	5	theme	purified	884:891	arg1	enzyme					893:898	the purified enzyme	880:898	the purified enzyme	880:898	Molybdenum activated the purified enzyme, and sodium dodecyl sulfate inhibited it.					
33783000	1	6	theme	glucoamylase	160:171	arg1	purification					121:132	purification	121:132	purification	121:132	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	1	6	theme	glucoamylase	160:171	arg1	characterization					138:153	characterization	138:153	characterization	138:153	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	9	7	theme	chromatography	955:968	arg1	analysis					970:977	A thin layer chromatography analysis	942:977	A thin layer chromatography analysis	942:977	A thin layer chromatography analysis revealed glucose as the enzyme's main starch hydrolysis product.					
33783000	1	8	theme	genus	241:245	arg1	section					254:260	the Aspergillus genus Cremei section	225:260	the Aspergillus genus Cremei section	225:260	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	7	9	theme	hydrolyzed	840:849	arg1	starch					851:856	hydrolyzed starch	840:856	hydrolyzed starch	840:856	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	5	10	theme	enzyme	629:634	arg1	5.0					660:662	5.0	660:662	5.0	660:662	The optimal enzyme pH and temperature were 5.0 and 60°C (at pH 5.0), respectively.					
33783000	5	10	theme	enzyme	629:634	arg1	pH					636:637	The optimal enzyme pH	617:637	The optimal enzyme pH	617:637	The optimal enzyme pH and temperature were 5.0 and 60°C (at pH 5.0), respectively.					
33783000	1	11	dep	purification	121:132	arg1	the					117:119	the	117:119	the	117:119	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	11	12	theme	CBM20	1375:1379	arg1	family					1381:1386	the CBM20 family	1371:1386	the CBM20 family	1371:1386	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	1	13	theme	Cremei	247:252	arg1	section					254:260	the Aspergillus genus Cremei section	225:260	the Aspergillus genus Cremei section	225:260	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	7	14	theme	starch	851:856	arg1	0.057 mg/min					824:835	0.057 mg/min	824:835	0.057 mg/min of hydrolyzed starch	824:856	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	2	15	theme	enzyme	271:276	arg1	∼3.5 U/ml					290:298	∼3.5 U/ml	290:298	∼3.5 U/ml	290:298	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	15	theme	enzyme	271:276	arg1	production					278:287	Maximum enzyme production	263:287	Maximum enzyme production (∼3.5 U/ml)	263:299	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	15	theme	enzyme	271:276	arg1	source					368:373	the carbon source	357:373	the carbon source	357:373	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	1	16	theme	section	254:260	arg1	wentii					190:195	Aspergillus wentii	178:195	Aspergillus wentii (strain PG18)	178:209	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	1	16	theme	section	254:260	arg1	species					214:220	a species	212:220	a species of the Aspergillus genus Cremei section	212:260	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	2	17	theme	Maximum	263:269	arg1	∼3.5 U/ml					290:298	∼3.5 U/ml	290:298	∼3.5 U/ml	290:298	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	17	theme	Maximum	263:269	arg1	production					278:287	Maximum enzyme production	263:287	Maximum enzyme production (∼3.5 U/ml)	263:299	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	17	theme	Maximum	263:269	arg1	source					368:373	the carbon source	357:373	the carbon source	357:373	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	10	18	theme	v1.0	1184:1187	arg1	genome					1164:1169	the annotated genome	1150:1169	the annotated genome of A. wentii v1.0	1150:1187	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	2	19	theme	carbon	361:366	arg1	production					278:287	Maximum enzyme production	263:287	Maximum enzyme production (∼3.5 U/ml)	263:299	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	19	theme	carbon	361:366	arg1	source					368:373	the carbon source	357:373	the carbon source	357:373	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	11	20	theme	structural	1203:1212	arg1	model					1214:1218	An in silico structural model	1190:1218	An in silico structural model	1190:1218	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	6	21	theme	enzyme	723:728	arg1	Tm					704:705	The Tm	700:705	The Tm of the purified enzyme	700:728	The Tm of the purified enzyme was 60°C, at pH 7.0.					
33783000	5	22	from	pH	677:678	arg1	5.0					660:662	5.0	660:662	5.0	660:662	The optimal enzyme pH and temperature were 5.0 and 60°C (at pH 5.0), respectively.					
33783000	5	22	from	pH	677:678	arg1	pH					636:637	The optimal enzyme pH	617:637	The optimal enzyme pH	617:637	The optimal enzyme pH and temperature were 5.0 and 60°C (at pH 5.0), respectively.					
33783000	2	23	theme	orbital	394:400	arg1	100 rpm					413:419	100 rpm	413:419	100 rpm	413:419	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	23	theme	orbital	394:400	arg1	agitation					402:410	orbital agitation	394:410	orbital agitation (100 rpm)	394:420	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	0	24	from	wentii	34:39	arg1	glucoamylase					4:15	The glucoamylase	0:15	The glucoamylase from Aspergillus wentii	0:39	The glucoamylase from Aspergillus wentii: Purification and characterization.					
33783000	6	25	theme	purified	714:721	arg1	enzyme					723:728	the purified enzyme	710:728	the purified enzyme	710:728	The Tm of the purified enzyme was 60°C, at pH 7.0.					
33783000	10	26	theme	A.	1174:1175	arg1	v1.0					1184:1187	A. wentii v1.0	1174:1187	A. wentii v1.0	1174:1187	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	11	27	theme	N-terminal	1231:1240	arg1	domain					1279:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	4	28	theme	purified	508:515	arg1	enzyme					517:522	The 86 kDa purified enzyme	497:522	The 86 kDa purified enzyme	497:522	The 86 kDa purified enzyme hydrolyzed starch in a zymogram and had activity against p-nitrophenyl α- d-glucopyranoside.					
33783000	11	29	theme	in	1193:1194	arg1	model					1214:1218	An in silico structural model	1190:1218	An in silico structural model	1190:1218	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	11	30	theme	carbohydrate-binding	1332:1351	arg1	CBM					1361:1363	CBM	1361:1363	CBM	1361:1363	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	11	30	theme	carbohydrate-binding	1332:1351	arg1	module					1353:1358	a C-terminal carbohydrate-binding module	1319:1358	a C-terminal carbohydrate-binding module (CBM) from the CBM20 family	1319:1386	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	4	31	theme	86 kDa	501:506	arg1	enzyme					517:522	The 86 kDa purified enzyme	497:522	The 86 kDa purified enzyme	497:522	The 86 kDa purified enzyme hydrolyzed starch in a zymogram and had activity against p-nitrophenyl α- d-glucopyranoside.					
33783000	7	32	theme	0.057 mg/min	824:835	arg1	KM					783:784	a KM	781:784	a KM for starch of 1.4 mg/ml	781:808	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	7	32	theme	0.057 mg/min	824:835	arg1	Vmax					816:819	a Vmax	814:819	a Vmax of 0.057 mg/min of hydrolyzed starch	814:856	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	1	33	theme	strain	198:203	arg1	wentii					190:195	Aspergillus wentii	178:195	Aspergillus wentii (strain PG18)	178:209	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	1	33	theme	strain	198:203	arg1	PG18					205:208	strain PG18	198:208	strain PG18	198:208	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	10	34	dep	A.	1174:1175	arg1	wentii					1177:1182	wentii	1177:1182	wentii	1177:1182	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	3	35	theme	exclusion	471:479	arg1	chromatography					481:494	one-step molecular exclusion chromatography	452:494	one-step molecular exclusion chromatography	452:494	The enzyme was purified with one-step molecular exclusion chromatography.					
33783000	1	36	theme	first	106:110	arg1	time					112:115	the first time	102:115	the first time	102:115	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	9	37	theme	main	1012:1015	arg1	product					1035:1041	the enzyme's main starch hydrolysis product	999:1041	the enzyme's main starch hydrolysis product	999:1041	A thin layer chromatography analysis revealed glucose as the enzyme's main starch hydrolysis product.					
33783000	4	38	theme	p-nitrophenyl	581:593	arg1	d-glucopyranoside					598:614	p-nitrophenyl α- d-glucopyranoside	581:614	p-nitrophenyl α- d-glucopyranoside	581:614	The 86 kDa purified enzyme hydrolyzed starch in a zymogram and had activity against p-nitrophenyl α- d-glucopyranoside.					
33783000	11	39	theme	family	1262:1267	arg1	domain					1279:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	10	40	theme	annotated	1154:1162	arg1	genome					1164:1169	the annotated genome	1150:1169	the annotated genome of A. wentii v1.0	1150:1187	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	9	41	theme	starch	1017:1022	arg1	product					1035:1041	the enzyme's main starch hydrolysis product	999:1041	the enzyme's main starch hydrolysis product	999:1041	A thin layer chromatography analysis revealed glucose as the enzyme's main starch hydrolysis product.					
33783000	11	42	dep	in	1193:1194	arg1	silico					1196:1201	silico	1196:1201	silico	1196:1201	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	2	43	with	culture	327:333	arg1	starch					347:352	starch	347:352	starch	347:352	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	0	44	dep	Purification	42:53	arg1	glucoamylase					4:15	The glucoamylase	0:15	The glucoamylase from Aspergillus wentii	0:39	The glucoamylase from Aspergillus wentii: Purification and characterization.					
33783000	9	45	theme	hydrolysis	1024:1033	arg1	product					1035:1041	the enzyme's main starch hydrolysis product	999:1041	the enzyme's main starch hydrolysis product	999:1041	A thin layer chromatography analysis revealed glucose as the enzyme's main starch hydrolysis product.					
33783000	11	46	from	family	1381:1386	arg1	CBM					1361:1363	CBM	1361:1363	CBM	1361:1363	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	11	46	from	family	1381:1386	arg1	module					1353:1358	a C-terminal carbohydrate-binding module	1319:1358	a C-terminal carbohydrate-binding module (CBM) from the CBM20 family	1319:1386	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	11	47	theme	glycosyl	1242:1249	arg1	domain					1279:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	10	48	theme	peptide	1056:1062	arg1	sequence					1064:1071	An enzyme's peptide sequence	1044:1071	An enzyme's peptide sequence	1044:1071	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	11	49	theme	hydrolases	1251:1260	arg1	domain					1279:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	a N-terminal glycosyl hydrolases family 15 (GH15) domain	1229:1284	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	2	50	theme	submerged	317:325	arg1	72 h					336:339	72 h	336:339	72 h	336:339	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	2	50	theme	submerged	317:325	arg1	culture					327:333	submerged culture	317:333	submerged culture (72 h) with starch	317:352	Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as the carbon source, at 25°C, and with orbital agitation (100 rpm).					
33783000	8	51	theme	dodecyl	912:918	arg1	sulfate					920:926	sodium dodecyl sulfate	905:926	sodium dodecyl sulfate	905:926	Molybdenum activated the purified enzyme, and sodium dodecyl sulfate inhibited it.					
33783000	8	52	theme	sodium	905:910	arg1	sulfate					920:926	sodium dodecyl sulfate	905:926	sodium dodecyl sulfate	905:926	Molybdenum activated the purified enzyme, and sodium dodecyl sulfate inhibited it.					
33783000	10	53	theme	mass	1089:1092	arg1	spectrometry					1094:1105	mass spectrometry	1089:1105	mass spectrometry	1089:1105	An enzyme's peptide sequence was obtained by mass spectrometry and used to retrieve a glucoamylase within the annotated genome of A. wentii v1.0.					
33783000	7	54	contain	had	777:779	arg2	Vmax					816:819	a Vmax	814:819	a Vmax of 0.057 mg/min of hydrolyzed starch	814:856	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	7	54	contain	had	777:779	arg1	glucoamylase					764:775	The purified glucoamylase	751:775	The purified glucoamylase	751:775	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	7	54	contain	had	777:779	arg2	KM					783:784	a KM	781:784	a KM for starch of 1.4 mg/ml	781:808	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	1	55	from	wentii	190:195	arg1	purification					121:132	purification	121:132	purification	121:132	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	1	55	from	wentii	190:195	arg1	characterization					138:153	characterization	138:153	characterization	138:153	This study describes for the first time the purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), a species of the Aspergillus genus Cremei section.					
33783000	7	56	theme	1.4 mg/ml	800:808	arg1	starch					790:795	starch	790:795	starch of 1.4 mg/ml	790:808	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	7	57	theme	purified	755:762	arg1	glucoamylase					764:775	The purified glucoamylase	751:775	The purified glucoamylase	751:775	The purified glucoamylase had a KM for starch of 1.4 mg/ml and a Vmax of 0.057 mg/min of hydrolyzed starch.					
33783000	4	58	theme	α-	595:596	arg1	d-glucopyranoside					598:614	p-nitrophenyl α- d-glucopyranoside	581:614	p-nitrophenyl α- d-glucopyranoside	581:614	The 86 kDa purified enzyme hydrolyzed starch in a zymogram and had activity against p-nitrophenyl α- d-glucopyranoside.					
33783000	3	59	theme	one-step	452:459	arg1	chromatography					481:494	one-step molecular exclusion chromatography	452:494	one-step molecular exclusion chromatography	452:494	The enzyme was purified with one-step molecular exclusion chromatography.					
33783000	11	60	theme	C-terminal	1321:1330	arg1	CBM					1361:1363	CBM	1361:1363	CBM	1361:1363	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	11	60	theme	C-terminal	1321:1330	arg1	module					1353:1358	a C-terminal carbohydrate-binding module	1319:1358	a C-terminal carbohydrate-binding module (CBM) from the CBM20 family	1319:1386	An in silico structural model revealed a N-terminal glycosyl hydrolases family 15 (GH15) domain, which is ligated by a linker to a C-terminal carbohydrate-binding module (CBM) from the CBM20 family.					
33783000	4	61	contain	had	560:562	arg2	activity					564:571	activity	564:571	activity	564:571	The 86 kDa purified enzyme hydrolyzed starch in a zymogram and had activity against p-nitrophenyl α- d-glucopyranoside.					
33783000	4	61	contain	had	560:562	arg1	enzyme					517:522	The 86 kDa purified enzyme	497:522	The 86 kDa purified enzyme	497:522	The 86 kDa purified enzyme hydrolyzed starch in a zymogram and had activity against p-nitrophenyl α- d-glucopyranoside.					
33783000	3	62	theme	molecular	461:469	arg1	chromatography					481:494	one-step molecular exclusion chromatography	452:494	one-step molecular exclusion chromatography	452:494	The enzyme was purified with one-step molecular exclusion chromatography.					
33783000	9	63	theme	thin	944:947	arg1	chromatography					955:968	A thin layer chromatography	942:968	A thin layer chromatography analysis	942:977	A thin layer chromatography analysis revealed glucose as the enzyme's main starch hydrolysis product.					
33867170	6	0	theme	physicochemical	971:985	arg1	properties					987:996	The physicochemical properties	967:996	The physicochemical properties	967:996	The physicochemical properties were assessed for biodegradation, swelling and total released proteins.					
33867170	13	1	dep	SIGNIFICANCE	1761:1772	arg1	chitosan					1778:1785	The chitosan	1774:1785	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin	1761:1846	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	1	2	theme	ideal	169:173	arg1	scaffold					175:182	An ideal scaffold	166:182	OBJECTIVE An ideal scaffold for endodontic regeneration	156:210	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	9	3	theme	Cell	1304:1307	arg1	differentiation					1309:1323	Cell differentiation	1304:1323	Cell differentiation in scaffolds	1304:1336	Cell differentiation in scaffolds was assessed by using alizarin red assay.					
33867170	7	4	theme	apical	1151:1156	arg1	hCAPs					1167:1171	hCAPs	1167:1171	hCAPs	1167:1171	The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs).					
33867170	7	4	theme	apical	1151:1156	arg1	papilla					1158:1164	the apical papilla	1147:1164	the apical papilla (hCAPs)	1147:1172	The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs).					
33867170	2	5	theme	scaffold	380:387	arg1	functionalization					355:371	functionalization	355:371	functionalization	355:371	Therefore, composition and functionalization of the scaffold play an important role in tissue bioengineering.					
33867170	2	5	theme	scaffold	380:387	arg1	composition					339:349	composition	339:349	composition	339:349	Therefore, composition and functionalization of the scaffold play an important role in tissue bioengineering.					
33867170	13	6	theme	suitable	1929:1936	arg1	characteristics					1913:1927	morphological, physicochemical, biological and antibacterial characteristics	1852:1927	morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics	1852:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	8	7	theme	Cell	1175:1178	arg1	morphology					1180:1189	Cell morphology	1175:1189	Cell morphology	1175:1189	Cell morphology and adhesion to the scaffolds were evaluated by SEM, cytotoxicity and cell proliferation by MTT reduction-assay.					
33867170	6	8	dep	biodegradation	1016:1029	arg1	proteins					1060:1067	released proteins	1051:1067	released proteins	1051:1067	The physicochemical properties were assessed for biodegradation, swelling and total released proteins.					
33867170	10	9	theme	culture	1459:1465	arg1	method					1467:1472	the bacterial culture method	1445:1472	the bacterial culture method	1445:1472	The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.					
33867170	10	10	theme	scaffolds	1412:1420	arg1	effect					1398:1403	The antimicrobial effect	1380:1403	The antimicrobial effect of the scaffolds	1380:1420	The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.					
33867170	3	11	theme	chitosan-based	571:584	arg1	scaffold					586:593	a new solid chitosan-based scaffold	559:593	a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	559:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	12	12	theme	Enterococcus	1710:1721	arg1	faecalis					1723:1730	Enterococcus faecalis	1710:1730	Enterococcus faecalis	1710:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	3	13	theme	scaffold	586:593	arg1	properties					545:554	the morphological, physicochemical, biological and antimicrobial properties	480:554	the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	480:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	3	14	theme	antimicrobial	531:543	arg1	properties					545:554	the morphological, physicochemical, biological and antimicrobial properties	480:554	the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	480:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	13	15	theme	physicochemical	1867:1881	arg1	characteristics					1913:1927	morphological, physicochemical, biological and antibacterial characteristics	1852:1927	morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics	1852:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	1	16	theme	negative	290:297	arg1	impact					299:304	the negative impact	286:304	the negative impact of residual bacteria	286:325	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	9	17	from	differentiation	1309:1323	arg1	scaffolds					1328:1336	scaffolds	1328:1336	scaffolds	1328:1336	Cell differentiation in scaffolds was assessed by using alizarin red assay.					
33867170	3	18	theme	new	561:563	arg1	scaffold					586:593	a new solid chitosan-based scaffold	559:593	a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	559:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	5	19	theme	electron	940:947	arg1	SEM					961:963	SEM	961:963	SEM	961:963	The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM).					
33867170	5	19	theme	electron	940:947	arg1	microscopy					949:958	scanning electron microscopy	931:958	scanning electron microscopy (SEM)	931:964	The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM).					
33867170	13	20	theme	biological	1884:1893	arg1	characteristics					1913:1927	morphological, physicochemical, biological and antibacterial characteristics	1852:1927	morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics	1852:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	7	21	theme	human	1130:1134	arg1	cells					1136:1140	human cells	1130:1140	human cells from the apical papilla (hCAPs)	1130:1172	The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs).					
33867170	12	22	theme	p	1750:1750	arg1	<					1752:1752	p < 0.05	1750:1757	p < 0.05	1750:1757	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	22	theme	p	1750:1750	arg1	Ch					1737:1738	Ch	1737:1738	Ch	1737:1738	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	3	23	theme	solid	565:569	arg1	scaffold					586:593	a new solid chitosan-based scaffold	559:593	a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	559:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	12	24	theme	bacteriostatic	1685:1698	arg1	effect					1700:1705	bacteriostatic effect	1685:1705	bacteriostatic effect on Enterococcus faecalis	1685:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	1	25	theme	endodontic	188:197	arg1	regeneration					199:210	endodontic regeneration	188:210	endodontic regeneration	188:210	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	10	26	theme	bacterial	1479:1487	arg1	adhesion					1489:1496	bacterial adhesion	1479:1496	bacterial adhesion to the scaffolds	1479:1513	The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.					
33867170	10	27	theme	bacterial	1449:1457	arg1	method					1467:1472	the bacterial culture method	1445:1472	the bacterial culture method	1445:1472	The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.					
33867170	13	28	from	use	1958:1960	arg1	endodontics					1990:2000	regenerative endodontics	1977:2000	regenerative endodontics	1977:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	13	29	theme	regenerative	1977:1988	arg1	endodontics					1990:2000	regenerative endodontics	1977:2000	regenerative endodontics	1977:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	8	30	theme	cell	1261:1264	arg1	proliferation					1266:1278	cell proliferation	1261:1278	cell proliferation	1261:1278	Cell morphology and adhesion to the scaffolds were evaluated by SEM, cytotoxicity and cell proliferation by MTT reduction-assay.					
33867170	4	31	theme	freeze-drying	861:873	arg1	method					875:880	the freeze-drying method	857:880	the freeze-drying method	857:880	METHODS Scaffolds based on chitosan (Ch); chitosan associated with gelatin and genipin (ChGG); and chitosan associated with gelatin, microparticulate dentin and genipin (ChGDG) were prepared by using the freeze-drying method.					
33867170	11	32	theme	porous	1572:1577	arg1	structures					1579:1588	porous structures	1572:1588	porous structures	1572:1588	RESULTS All the scaffolds presented porous structures.					
33867170	12	33	from	differentiation	1655:1669	arg1	faecalis					1723:1730	Enterococcus faecalis	1710:1730	Enterococcus faecalis	1710:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	3	34	theme	study	460:464	arg1	objective					442:450	The objective	438:450	The objective of this study	438:464	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	9	35	theme	alizarin	1360:1367	arg1	assay					1373:1377	alizarin red assay	1360:1377	alizarin red assay	1360:1377	Cell differentiation in scaffolds was assessed by using alizarin red assay.					
33867170	0	36	theme	endodontic	131:140	arg1	regeneration					142:153	endodontic regeneration	131:153	endodontic regeneration	131:153	Development and characterization of a new chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin for endodontic regeneration.					
33867170	11	37	dep	RESULTS	1536:1542	arg1	scaffolds					1552:1560	All the scaffolds	1544:1560	RESULTS All the scaffolds	1536:1560	RESULTS All the scaffolds presented porous structures.					
33867170	1	38	dep	OBJECTIVE	156:164	arg1	scaffold					175:182	An ideal scaffold	166:182	OBJECTIVE An ideal scaffold for endodontic regeneration	156:210	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	3	39	theme	morphological	484:496	arg1	properties					545:554	the morphological, physicochemical, biological and antimicrobial properties	480:554	the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	480:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	1	40	theme	residual	309:316	arg1	bacteria					318:325	residual bacteria	309:325	residual bacteria	309:325	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	7	41	theme	scaffolds	1100:1108	arg1	aspects					1085:1091	The biological aspects	1070:1091	The biological aspects of the scaffolds	1070:1108	The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs).					
33867170	12	42	from	proliferation	1637:1649	arg1	faecalis					1723:1730	Enterococcus faecalis	1710:1730	Enterococcus faecalis	1710:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	13	43	theme	morphological	1852:1864	arg1	characteristics					1913:1927	morphological, physicochemical, biological and antibacterial characteristics	1852:1927	morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics	1852:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	0	44	theme	new	38:40	arg1	scaffold					57:64	a new chitosan-based scaffold	36:64	a new chitosan-based scaffold associated with gelatin	36:88	Development and characterization of a new chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin for endodontic regeneration.					
33867170	1	45	theme	bacteria	318:325	arg1	impact					299:304	the negative impact	286:304	the negative impact of residual bacteria	286:325	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	12	46	from	adhesion	1627:1634	arg1	faecalis					1723:1730	Enterococcus faecalis	1710:1730	Enterococcus faecalis	1710:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	9	47	theme	red	1369:1371	arg1	assay					1373:1377	alizarin red assay	1360:1377	alizarin red assay	1360:1377	Cell differentiation in scaffolds was assessed by using alizarin red assay.					
33867170	6	48	theme	released	1051:1058	arg1	proteins					1060:1067	released proteins	1051:1067	released proteins	1051:1067	The physicochemical properties were assessed for biodegradation, swelling and total released proteins.					
33867170	12	49	from	release	1618:1624	arg1	faecalis					1723:1730	Enterococcus faecalis	1710:1730	Enterococcus faecalis	1710:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	50	theme	hCAPs	1674:1678	arg1	differentiation					1655:1669	differentiation	1655:1669	differentiation	1655:1669	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	50	theme	hCAPs	1674:1678	arg1	proliferation					1637:1649	proliferation	1637:1649	proliferation	1637:1649	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	50	theme	hCAPs	1674:1678	arg1	adhesion					1627:1634	adhesion	1627:1634	adhesion	1627:1634	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	50	theme	hCAPs	1674:1678	arg1	effect					1700:1705	bacteriostatic effect	1685:1705	bacteriostatic effect on Enterococcus faecalis	1685:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	50	theme	hCAPs	1674:1678	arg1	release					1618:1624	more protein release	1605:1624	more protein release	1605:1624	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	8	51	theme	MTT	1283:1285	arg1	reduction-assay					1287:1301	MTT reduction-assay	1283:1301	MTT reduction-assay	1283:1301	Cell morphology and adhesion to the scaffolds were evaluated by SEM, cytotoxicity and cell proliferation by MTT reduction-assay.					
33867170	0	52	theme	scaffold	57:64	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of a new chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin for endodontic regeneration.					
33867170	0	52	theme	scaffold	57:64	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of a new chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin for endodontic regeneration.					
33867170	3	53	theme	microparticulate	620:635	arg1	dentin					637:642	microparticulate dentin	620:642	microparticulate dentin	620:642	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	3	54	theme	biological	516:525	arg1	properties					545:554	the morphological, physicochemical, biological and antimicrobial properties	480:554	the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	480:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	0	55	theme	chitosan-based	42:55	arg1	scaffold					57:64	a new chitosan-based scaffold	36:64	a new chitosan-based scaffold associated with gelatin	36:88	Development and characterization of a new chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin for endodontic regeneration.					
33867170	12	56	theme	protein	1610:1616	arg1	release					1618:1624	more protein release	1605:1624	more protein release	1605:1624	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	7	57	theme	biological	1074:1083	arg1	aspects					1085:1091	The biological aspects	1070:1091	The biological aspects of the scaffolds	1070:1108	The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs).					
33867170	5	58	theme	scanning	931:938	arg1	SEM					961:963	SEM	961:963	SEM	961:963	The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM).					
33867170	5	58	theme	scanning	931:938	arg1	microscopy					949:958	scanning electron microscopy	931:958	scanning electron microscopy (SEM)	931:964	The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM).					
33867170	12	59	theme	more	1605:1608	arg1	release					1618:1624	more protein release	1605:1624	more protein release	1605:1624	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	4	60	dep	associated	708:717	arg1	Scaffolds					665:673	METHODS Scaffolds	657:673	METHODS Scaffolds based on chitosan (Ch)	657:696	METHODS Scaffolds based on chitosan (Ch); chitosan associated with gelatin and genipin (ChGG); and chitosan associated with gelatin, microparticulate dentin and genipin (ChGDG) were prepared by using the freeze-drying method.					
33867170	12	61	contain	had	1601:1603	arg2	effect					1700:1705	bacteriostatic effect	1685:1705	bacteriostatic effect on Enterococcus faecalis	1685:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	61	contain	had	1601:1603	arg1	ChCDG					1595:1599	The ChCDG	1591:1599	The ChCDG	1591:1599	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	61	contain	had	1601:1603	arg2	adhesion					1627:1634	adhesion	1627:1634	adhesion	1627:1634	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	61	contain	had	1601:1603	arg2	release					1618:1624	more protein release	1605:1624	more protein release	1605:1624	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	12	61	contain	had	1601:1603	arg2	proliferation					1637:1649	proliferation	1637:1649	proliferation	1637:1649	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	3	62	theme	physicochemical	499:513	arg1	properties					545:554	the morphological, physicochemical, biological and antimicrobial properties	480:554	the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin	480:654	The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.					
33867170	13	63	theme	potential	1948:1956	arg1	use					1958:1960	their potential use	1942:1960	their potential use as scaffold in regenerative endodontics	1942:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	4	64	theme	METHODS	657:663	arg1	Scaffolds					665:673	METHODS Scaffolds	657:673	METHODS Scaffolds based on chitosan (Ch)	657:696	METHODS Scaffolds based on chitosan (Ch); chitosan associated with gelatin and genipin (ChGG); and chitosan associated with gelatin, microparticulate dentin and genipin (ChGDG) were prepared by using the freeze-drying method.					
33867170	4	65	theme	microparticulate	790:805	arg1	dentin					807:812	microparticulate dentin	790:812	microparticulate dentin	790:812	METHODS Scaffolds based on chitosan (Ch); chitosan associated with gelatin and genipin (ChGG); and chitosan associated with gelatin, microparticulate dentin and genipin (ChGDG) were prepared by using the freeze-drying method.					
33867170	13	66	contain	has	1848:1850	arg1	SIGNIFICANCE					1761:1772	SIGNIFICANCE	1761:1772	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin	1761:1846	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	13	66	contain	has	1848:1850	arg2	characteristics					1913:1927	morphological, physicochemical, biological and antibacterial characteristics	1852:1927	morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics	1852:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	1	67	theme	new	252:254	arg1	organization					263:274	the new tissue organization	248:274	the new tissue organization	248:274	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	10	68	theme	antimicrobial	1384:1396	arg1	effect					1398:1403	The antimicrobial effect	1380:1403	The antimicrobial effect of the scaffolds	1380:1420	The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.					
33867170	2	69	theme	important	397:405	arg1	role					407:410	an important role	394:410	an important role	394:410	Therefore, composition and functionalization of the scaffold play an important role in tissue bioengineering.					
33867170	2	70	theme	tissue	415:420	arg1	bioengineering					422:435	tissue bioengineering	415:435	tissue bioengineering	415:435	Therefore, composition and functionalization of the scaffold play an important role in tissue bioengineering.					
33867170	1	71	theme	tissue	256:261	arg1	organization					263:274	the new tissue organization	248:274	the new tissue organization	248:274	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
33867170	13	72	theme	microparticulate	1812:1827	arg1	dentin					1829:1834	microparticulate dentin	1812:1834	microparticulate dentin	1812:1834	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	7	73	from	papilla	1158:1164	arg1	cells					1136:1140	human cells	1130:1140	human cells from the apical papilla (hCAPs)	1130:1172	The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs).					
33867170	12	74	from	effect	1700:1705	arg1	faecalis					1723:1730	Enterococcus faecalis	1710:1730	Enterococcus faecalis	1710:1730	The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).					
33867170	5	75	theme	scaffolds	905:913	arg1	morphology					887:896	The morphology	883:896	The morphology of the scaffolds	883:913	The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM).					
33867170	13	76	theme	antibacterial	1899:1911	arg1	characteristics					1913:1927	morphological, physicochemical, biological and antibacterial characteristics	1852:1927	morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics	1852:2000	SIGNIFICANCE The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.					
33867170	1	77	theme	organization	263:274	arg1	predictableness					229:243	the predictableness	225:243	the predictableness of the new tissue organization	225:274	OBJECTIVE An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria.					
34357369	2	0	theme	simultaneous	389:400	arg1	removal					416:422	the simultaneous and efficient removal	385:422	the simultaneous and efficient removal of multiple co-existing pollutants	385:457	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	0	1	theme	simultaneous	81:92	arg1	removal					94:100	simultaneous removal	81:100	simultaneous removal of multiple pollutants	81:123	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	1	2	theme	BC	171:172	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	0	3	theme	multiple	105:112	arg1	pollutants					114:123	multiple pollutants	105:123	multiple pollutants	105:123	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	1	4	theme	facile	295:300	arg1	strategy					312:319	a facile versatile strategy	293:319	a facile versatile strategy	293:319	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	2	5	theme	co-existing	436:446	arg1	pollutants					448:457	multiple co-existing pollutants	427:457	multiple co-existing pollutants	427:457	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	0	6	theme	pollutants	114:123	arg1	removal					94:100	simultaneous removal	81:100	simultaneous removal of multiple pollutants	81:123	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	1	7	dep	self-standing	128:140	arg1	robust					143:148	robust	143:148	robust	143:148	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	1	8	theme	-based	174:179	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	0	9	theme	universal	2:10	arg1	route					12:16	A universal route	0:16	A universal route	0:16	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	2	10	theme	developed	339:347	arg1	membrane					368:375	the developed BC-based composite membrane	335:375	the developed BC-based composite membrane	335:375	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	1	11	theme	multifunctional	181:195	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	1	12	theme	versatile	302:310	arg1	strategy					312:319	a facile versatile strategy	293:319	a facile versatile strategy	293:319	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	1	13	theme	composite	197:205	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	2	14	theme	efficient	406:414	arg1	removal					416:422	the simultaneous and efficient removal	385:422	the simultaneous and efficient removal of multiple co-existing pollutants	385:457	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	1	15	theme	embedded	216:223	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	0	16	theme	bacterial	31:39	arg1	membranes					67:75	bacterial cellulose-based composite membranes	31:75	bacterial cellulose-based composite membranes	31:75	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	1	17	with	embedded	216:223	arg1	nano-adsorbents					240:254	desirable nano-adsorbents	230:254	desirable nano-adsorbents	230:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	1	18	theme	desirable	230:238	arg1	nano-adsorbents					240:254	desirable nano-adsorbents	230:254	desirable nano-adsorbents	230:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	0	19	theme	composite	57:65	arg1	membranes					67:75	bacterial cellulose-based composite membranes	31:75	bacterial cellulose-based composite membranes	31:75	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	0	20	theme	cellulose-based	41:55	arg1	membranes					67:75	bacterial cellulose-based composite membranes	31:75	bacterial cellulose-based composite membranes	31:75	A universal route to fabricate bacterial cellulose-based composite membranes for simultaneous removal of multiple pollutants.					
34357369	1	21	theme	self-standing	128:140	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	2	22	theme	composite	358:366	arg1	membrane					368:375	the developed BC-based composite membrane	335:375	the developed BC-based composite membrane	335:375	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	2	23	theme	BC-based	349:356	arg1	membrane					368:375	the developed BC-based composite membrane	335:375	the developed BC-based composite membrane	335:375	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	2	24	theme	pollutants	448:457	arg1	removal					416:422	the simultaneous and efficient removal	385:422	the simultaneous and efficient removal of multiple co-existing pollutants	385:457	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	1	25	theme	bacterial	150:158	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34357369	2	26	theme	multiple	427:434	arg1	pollutants					448:457	multiple co-existing pollutants	427:457	multiple co-existing pollutants	427:457	As expected, the developed BC-based composite membrane enables the simultaneous and efficient removal of multiple co-existing pollutants.					
34357369	1	27	theme	cellulose	160:168	arg1	membrane					207:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane	126:214	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents	126:254	A self-standing, robust bacterial cellulose (BC)-based multifunctional composite membrane embedded with desirable nano-adsorbents has been successfully fabricated via a facile versatile strategy.					
34730949	11	0	theme	free	2049:2052	arg1	energy					2054:2059	standard Gibbs free energy	2034:2059	standard Gibbs free energy	2034:2059	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	4	1	theme	circular	487:494	arg1	dichroism					496:504	circular dichroism	487:504	circular dichroism (CD)	487:509	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	1	theme	circular	487:494	arg1	CD					507:508	CD	507:508	CD	507:508	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	6	2	theme	well-defined	1099:1110	arg1	sites					1112:1116	conformationally well-defined sites	1082:1116	conformationally well-defined sites on the polymer	1082:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	7	3	theme	right	1300:1304	arg1	association					1288:1298	chain association	1282:1298	chain association right from the beginning of addition to pectate	1282:1346	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	10	4	theme	polyelectrolytes	1968:1983	arg1	association					1940:1950	the association	1936:1950	the association of semiflexible polyelectrolytes	1936:1983	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	6	5	theme	covalent	1045:1052	arg1	bonding					1054:1060	not a covalent bonding	1039:1060	not a covalent bonding	1039:1060	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	6	theme	strong	1014:1019	arg1	bonding					1030:1036	strong chemical bonding	1014:1036	strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer	1014:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	11	7	theme	standard	2034:2041	arg1	energy					2054:2059	standard Gibbs free energy	2034:2059	standard Gibbs free energy	2034:2059	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	2	8	theme	acid/sodium	137:147	arg1	pectate					149:155	Pectic acid/sodium pectate	130:155	Pectic acid/sodium pectate	130:155	Pectic acid/sodium pectate is one of the most widespread hydrocolloid used in the food industry.					
34730949	14	9	from	conformation	2715:2726	arg1	solution					2757:2764	aqueous solution	2749:2764	aqueous solution	2749:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	7	10	theme	chain	1282:1286	arg1	association					1288:1298	chain association	1282:1298	chain association right from the beginning of addition to pectate	1282:1346	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	7	11	theme	Rj	1238:1239	arg1	function					1226:1233	a function	1224:1233	a function of Rj	1224:1239	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	10	12	theme	bonding	1839:1845	arg1	categories					1847:1856	similar bonding categories	1831:1856	similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes	1831:1983	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	13	13	theme	gels	2576:2579	arg1	elasticity					2549:2558	the elasticity	2545:2558	the elasticity of Ca2+-pectate gels	2545:2579	This finding corroborated previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels.					
34730949	14	14	theme	different	2615:2623	arg1	techniques					2625:2634	different techniques	2615:2634	different techniques	2615:2634	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	12	15	theme	bonding	2446:2452	arg1	process					2454:2460	the type-2 bonding process	2435:2460	the type-2 bonding process	2435:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	16	theme	LMP	2246:2248	arg1	Tg					2239:2240	Tg	2239:2240	Tg	2239:2240	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	16	theme	LMP	2246:2248	arg1	temperatures					2225:2236	the gelation temperatures	2212:2236	the gelation temperatures	2212:2236	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	17	theme	association	2371:2381	arg1	free-energy					2337:2347	standard Gibbs free-energy	2322:2347	standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process	2322:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	17	theme	association	2371:2381	arg1	values					2305:2310	the values	2301:2310	the values of Tg	2301:2316	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	6	18	with	theory	816:821	arg1	annihilation					1152:1163	local charge annihilation	1139:1163	local charge annihilation	1139:1163	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	9	19	theme	shifted	1658:1664	arg1	"					1673:1673	the "shifted egg-box"	1653:1673	the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation	1653:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	6	20	theme	charge	1145:1150	arg1	annihilation					1152:1163	local charge annihilation	1139:1163	local charge annihilation	1139:1163	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	14	21	from	solution	2757:2764	arg1	conformation					2715:2726	the starting conformation	2702:2726	the starting conformation of sodium pectate in aqueous solution	2702:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	14	21	from	solution	2757:2764	arg1	"					2697:2697	a "loose-21-helix"	2680:2697	a "loose-21-helix"	2680:2697	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	9	22	theme	first	1500:1504	arg1	type					1512:1515	type 1	1512:1517	type 1	1512:1517	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	9	22	theme	first	1500:1504	arg1	mode					1506:1509	the first mode	1496:1509	the first mode (type 1)	1496:1518	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	7	23	theme	mass	1211:1214	arg1	data					1216:1219	Intrinsic viscosity and number-average molar mass data	1166:1219	Intrinsic viscosity and number-average molar mass data as a function of Rj	1166:1239	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	5	24	theme	aqueous	717:723	arg1	NaClO4					725:730	aqueous NaClO4	717:730	aqueous NaClO4	717:730	Data were collected in aqueous NaClO4 at 25 °C.					
34730949	6	25	dep	bonding	1030:1036	arg1	bonding					1054:1060	not a covalent bonding	1039:1060	not a covalent bonding	1039:1060	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	26	theme	condensation	803:814	arg1	instrument					750:759	The key instrument	742:759	The key instrument of interpretation	742:777	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	26	theme	condensation	803:814	arg1	theory					816:821	the counterion condensation theory	788:821	the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"),	788:989	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	26	theme	condensation	803:814	arg1	CCT					824:826	CCT	824:826	CCT	824:826	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	12	27	theme	Gibbs	2331:2335	arg1	free-energy					2337:2347	standard Gibbs free-energy	2322:2347	standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process	2322:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	4	28	theme	pectate	384:390	arg1	sample					367:372	a sample	365:372	a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate)	365:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	7	29	theme	number-average	1190:1203	arg1	mass					1211:1214	number-average molar mass	1190:1214	number-average molar mass	1190:1214	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	4	30	theme	increasing	595:604	arg1	Rj					606:607	increasing Rj	595:607	increasing Rj	595:607	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	13	31	theme	elasticity	2549:2558	arg1	nature					2535:2540	the enthalpic nature	2521:2540	the enthalpic nature of the elasticity of Ca2+-pectate gels	2521:2579	This finding corroborated previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels.					
34730949	1	32	theme	Calcium	105:111	arg1	Ions					113:116	Calcium Ions	105:116	Calcium Ions	105:116	Different Steps in the Binding of Calcium Ions by Pectate.					
34730949	3	33	theme	ionotropic	253:262	arg1	gels					264:267	strong ionotropic gels	246:267	strong ionotropic gels	246:267	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	7	34	theme	viscosity	1176:1184	arg1	data					1216:1219	Intrinsic viscosity and number-average molar mass data	1166:1219	Intrinsic viscosity and number-average molar mass data as a function of Rj	1166:1239	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	10	35	theme	low-methoxyl	1884:1895	arg1	LMP					1905:1907	LMP	1905:1907	LMP	1905:1907	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	10	35	theme	low-methoxyl	1884:1895	arg1	pectin					1897:1902	low-methoxyl pectin	1884:1902	low-methoxyl pectin (LMP)	1884:1908	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	12	36	theme	Tg	2315:2316	arg1	free-energy					2337:2347	standard Gibbs free-energy	2322:2347	standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process	2322:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	36	theme	Tg	2315:2316	arg1	values					2305:2310	the values	2301:2310	the values of Tg	2301:2316	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	3	37	theme	ions	288:291	arg1	addition					276:283	the addition	272:283	the addition of ions	272:291	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	12	38	theme	dependence	2198:2207	arg1	analysis					2182:2189	the analysis	2178:2189	the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions	2178:2290	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	39	theme	calcium	2279:2285	arg1	ions					2287:2290	calcium ions	2279:2290	calcium ions	2279:2290	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	13	40	theme	enthalpic	2525:2533	arg1	nature					2535:2540	the enthalpic nature	2521:2540	the enthalpic nature of the elasticity of Ca2+-pectate gels	2521:2579	This finding corroborated previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels.					
34730949	14	41	theme	sodium	2731:2736	arg1	pectate					2738:2744	sodium pectate	2731:2744	sodium pectate in aqueous solution	2731:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	12	42	theme	type-2	2439:2444	arg1	process					2454:2460	the type-2 bonding process	2435:2460	the type-2 bonding process	2435:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	11	43	theme	bonding	2144:2150	arg1	processes					2152:2160	the chemical bonding processes	2131:2160	the chemical bonding processes	2131:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	9	44	theme	analysis	1735:1742	arg1	investigation					1744:1756	conformational analysis investigation	1720:1756	conformational analysis investigation	1720:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	13	45	theme	reported	2500:2507	arg1	evidence					2509:2516	previously reported evidence	2489:2516	previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels	2489:2579	This finding corroborated previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels.					
34730949	6	46	theme	linear	832:837	arg1	polyelectrolytes					839:854	linear polyelectrolytes	832:854	linear polyelectrolytes	832:854	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	9	47	theme	tilted	1550:1555	arg1	type					1566:1569	the "tilted egg-box" type	1545:1569	the "tilted egg-box" type	1545:1569	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	4	48	theme	concentrations	642:655	arg1	Rj					610:611	Rj	610:611	Rj	610:611	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	48	theme	concentrations	642:655	arg1	ratio					623:627	the ratio	619:627	the ratio of the molar concentrations of Ca2+ and pectate repeating units	619:691	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	1	49	theme	Different	71:79	arg1	Steps					81:85	Different Steps	71:85	Different Steps in the Binding of Calcium Ions by Pectate	71:127	Different Steps in the Binding of Calcium Ions by Pectate.					
34730949	6	50	theme	divalent	923:930	arg1	counterion					932:941	the divalent counterion	919:941	the divalent counterion	919:941	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	9	51	theme	"	1564:1564	arg1	type					1566:1569	the "tilted egg-box" type	1545:1569	the "tilted egg-box" type	1545:1569	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	11	52	theme	volume	2085:2090	arg1	values					2098:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values	2034:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes	2034:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	6	53	theme	key	746:748	arg1	theory					816:821	the counterion condensation theory	788:821	the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"),	788:989	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	53	theme	key	746:748	arg1	instrument					750:759	The key instrument	742:759	The key instrument of interpretation	742:777	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	11	54	dep	affinity	2118:2125	arg1	both					2105:2108	both	2105:2108	both	2105:2108	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	12	55	theme	increasing	2255:2264	arg1	additions					2266:2274	increasing additions	2255:2274	increasing additions of calcium ions	2255:2290	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	0	56	theme	Molecular	7:15	arg1	Mechanism					17:25	the Molecular Mechanism	3:25	the Molecular Mechanism of the Calcium-Induced Gelation of Pectate	3:68	On the Molecular Mechanism of the Calcium-Induced Gelation of Pectate.					
34730949	11	57	theme	molar	2092:2096	arg1	values					2098:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values	2034:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes	2034:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	6	58	theme	specific	898:905	arg1	affinity					907:914	both specific affinity	893:914	both specific affinity of the divalent counterion for the polysaccharide ("territorial binding")	893:988	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	8	59	theme	microcalorimetric	1369:1385	arg1	curve					1387:1391	the microcalorimetric curve	1365:1391	the microcalorimetric curve using the modified CCT	1365:1414	The analysis of the microcalorimetric curve using the modified CCT revealed two types of bonding.					
34730949	4	60	theme	units	687:691	arg1	concentrations					642:655	the molar concentrations	632:655	the molar concentrations of Ca2+ and pectate repeating units	632:691	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	9	61	theme	Ca2+	1594:1597	arg1	binding					1599:1605	Ca2+ binding	1594:1605	Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation	1594:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	4	62	theme	Na+-poly	433:440	arg1	galacturonate					442:454	galacturonate	442:454	galacturonate	442:454	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	62	theme	Na+-poly	433:440	arg1	%					422:422	90%	420:422	90% of ideal Na+-poly(galacturonate)	420:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	62	theme	Na+-poly	433:440	arg1	Na+-poly					433:440	ideal Na+-poly	427:440	ideal Na+-poly(galacturonate)	427:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	0	63	theme	Calcium-Induced	34:48	arg1	Gelation					50:57	the Calcium-Induced Gelation	30:57	the Calcium-Induced Gelation of Pectate	30:68	On the Molecular Mechanism of the Calcium-Induced Gelation of Pectate.					
34730949	7	64	theme	addition	1328:1335	arg1	beginning					1315:1323	the beginning	1311:1323	the beginning of addition to pectate	1311:1346	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	6	65	theme	binding	980:986	arg1	polysaccharide					951:964	the polysaccharide	947:964	the polysaccharide ("territorial binding")	947:988	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	65	theme	binding	980:986	arg1	"					987:987	"territorial binding"	967:987	"territorial binding"	967:987	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	12	66	theme	gelation	2216:2223	arg1	Tg					2239:2240	Tg	2239:2240	Tg	2239:2240	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	12	66	theme	gelation	2216:2223	arg1	temperatures					2225:2236	the gelation temperatures	2212:2236	the gelation temperatures	2212:2236	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	8	67	theme	modified	1403:1410	arg1	CCT					1412:1414	the modified CCT	1399:1414	the modified CCT	1399:1414	The analysis of the microcalorimetric curve using the modified CCT revealed two types of bonding.					
34730949	6	68	from	theory	816:821	arg1	sites					1112:1116	conformationally well-defined sites	1082:1116	conformationally well-defined sites on the polymer	1082:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	2	69	theme	widespread	176:185	arg1	hydrocolloid					187:198	the most widespread hydrocolloid	167:198	the most widespread hydrocolloid used in the food industry	167:224	Pectic acid/sodium pectate is one of the most widespread hydrocolloid used in the food industry.					
34730949	4	70	theme	Ca2+	660:663	arg1	concentrations					642:655	the molar concentrations	632:655	the molar concentrations of Ca2+ and pectate repeating units	632:691	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	14	71	from	pectate	2738:2744	arg1	solution					2757:2764	aqueous solution	2749:2764	aqueous solution	2749:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	3	72	dep	able	233:236	arg1	ions					317:320	calcium ions	309:320	calcium ions	309:320	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	4	73	theme	pectate	669:675	arg1	units					687:691	pectate repeating units	669:691	pectate repeating units	669:691	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	6	74	from	sites	1112:1116	arg1	polymer					1125:1131	the polymer	1121:1131	the polymer	1121:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	13	75	from	evidence	2509:2516	arg1	nature					2535:2540	the enthalpic nature	2521:2540	the enthalpic nature of the elasticity of Ca2+-pectate gels	2521:2579	This finding corroborated previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels.					
34730949	9	76	theme	second	1627:1632	arg1	mode					1634:1637	the second mode	1623:1637	the second mode (type 2)	1623:1646	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	9	76	theme	second	1627:1632	arg1	type					1640:1643	type 2	1640:1645	type 2	1640:1645	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	14	77	theme	aqueous	2749:2755	arg1	solution					2757:2764	aqueous solution	2749:2764	aqueous solution	2749:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	3	78	from	able	233:236	arg1	particular					297:306	particular	297:306	particular	297:306	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	6	79	theme	interpretation	764:777	arg1	theory					816:821	the counterion condensation theory	788:821	the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"),	788:989	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	79	theme	interpretation	764:777	arg1	instrument					750:759	The key instrument	742:759	The key instrument of interpretation	742:777	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	8	80	theme	bonding	1438:1444	arg1	types					1429:1433	two types	1425:1433	two types of bonding	1425:1444	The analysis of the microcalorimetric curve using the modified CCT revealed two types of bonding.					
34730949	9	81	with	mode	1634:1637	arg1	"					1673:1673	the "shifted egg-box"	1653:1673	the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation	1653:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	14	82	theme	CD	2644:2645	arg1	analysis					2603:2610	comparative analysis	2591:2610	comparative analysis of different techniques, but of CD	2591:2645	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	13	83	theme	Ca2+-pectate	2563:2574	arg1	gels					2576:2579	Ca2+-pectate gels	2563:2579	Ca2+-pectate gels	2563:2579	This finding corroborated previously reported evidence on the enthalpic nature of the elasticity of Ca2+-pectate gels.					
34730949	10	84	theme	semiflexible	1955:1966	arg1	polyelectrolytes					1968:1983	semiflexible polyelectrolytes	1955:1983	semiflexible polyelectrolytes	1955:1983	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	4	85	theme	Ca2+	352:355	arg1	ions					357:360	Ca2+ ions	352:360	Ca2+ ions	352:360	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	7	86	theme	calcium	1253:1259	arg1	bonding					1261:1267	calcium bonding	1253:1267	calcium bonding	1253:1267	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	2	87	theme	Pectic	130:135	arg1	pectate					149:155	Pectic acid/sodium pectate	130:155	Pectic acid/sodium pectate	130:155	Pectic acid/sodium pectate is one of the most widespread hydrocolloid used in the food industry.					
34730949	6	88	theme	Ca2+	1074:1077	arg1	instrument					750:759	The key instrument	742:759	The key instrument of interpretation	742:777	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	88	theme	Ca2+	1074:1077	arg1	theory					816:821	the counterion condensation theory	788:821	the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"),	788:989	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	88	theme	Ca2+	1074:1077	arg1	bonding					1030:1036	strong chemical bonding	1014:1036	strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer	1014:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	88	theme	Ca2+	1074:1077	arg1	CCT					824:826	CCT	824:826	CCT	824:826	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	10	89	theme	similar	1831:1837	arg1	categories					1847:1856	similar bonding categories	1831:1856	similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes	1831:1983	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	9	90	with	alginate	1610:1617	arg1	"					1673:1673	the "shifted egg-box"	1653:1673	the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation	1653:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	14	91	theme	techniques	2625:2634	arg1	analysis					2603:2610	comparative analysis	2591:2610	comparative analysis of different techniques, but of CD	2591:2645	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	11	92	theme	chemical	2135:2142	arg1	processes					2152:2160	the chemical bonding processes	2131:2160	the chemical bonding processes	2131:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	9	93	theme	Rj	1492:1493	arg1	function					1480:1487	a function	1478:1487	a function of Rj	1478:1493	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	4	94	theme	initial	327:333	arg1	function					583:590	a function	581:590	a function of increasing Rj	581:607	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	94	theme	initial	327:333	arg1	steps					335:339	The initial steps	323:339	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate)	323:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	95	theme	membrane	558:565	arg1	osmometry					567:575	membrane osmometry	558:575	membrane osmometry	558:575	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	11	96	theme	enthalpy	2062:2069	arg1	values					2098:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values	2034:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes	2034:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	3	97	theme	strong	246:251	arg1	gels					264:267	strong ionotropic gels	246:267	strong ionotropic gels	246:267	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	4	98	theme	sodium	377:382	arg1	pectate					384:390	sodium pectate	377:390	sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate)	377:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	9	99	theme	egg-box	1666:1672	arg1	"					1673:1673	the "shifted egg-box"	1653:1673	the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation	1653:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	6	100	theme	local	1139:1143	arg1	annihilation					1152:1163	local charge annihilation	1139:1163	local charge annihilation	1139:1163	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	3	101	from	particular	297:306	arg1	able					233:236	able	233:236	able	233:236	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	2	102	theme	food	212:215	arg1	industry					217:224	the food industry	208:224	the food industry	208:224	Pectic acid/sodium pectate is one of the most widespread hydrocolloid used in the food industry.					
34730949	6	103	theme	counterion	792:801	arg1	instrument					750:759	The key instrument	742:759	The key instrument of interpretation	742:777	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	103	theme	counterion	792:801	arg1	theory					816:821	the counterion condensation theory	788:821	the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"),	788:989	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	103	theme	counterion	792:801	arg1	CCT					824:826	CCT	824:826	CCT	824:826	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	12	104	theme	standard	2322:2329	arg1	free-energy					2337:2347	standard Gibbs free-energy	2322:2347	standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process	2322:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	7	105	theme	molar	1205:1209	arg1	mass					1211:1214	number-average molar mass	1190:1214	number-average molar mass	1190:1214	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	9	106	theme	calcium	1688:1694	arg1	pectate					1696:1702	calcium pectate	1688:1702	calcium pectate	1688:1702	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	10	107	with	superimposable	1811:1824	arg1	categories					1847:1856	similar bonding categories	1831:1856	similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes	1831:1983	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	1	108	theme	Ions	113:116	arg1	Binding					94:100	the Binding	90:100	the Binding of Calcium Ions by Pectate	90:127	Different Steps in the Binding of Calcium Ions by Pectate.					
34730949	12	109	theme	ions	2287:2290	arg1	additions					2266:2274	increasing additions	2255:2274	increasing additions of calcium ions	2255:2290	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	4	110	theme	Rj	606:607	arg1	function					583:590	a function	581:590	a function of increasing Rj	581:607	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	110	theme	Rj	606:607	arg1	steps					335:339	The initial steps	323:339	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate)	323:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	0	111	theme	Pectate	62:68	arg1	Gelation					50:57	the Calcium-Induced Gelation	30:57	the Calcium-Induced Gelation of Pectate	30:68	On the Molecular Mechanism of the Calcium-Induced Gelation of Pectate.					
34730949	7	112	theme	Intrinsic	1166:1174	arg1	viscosity					1176:1184	Intrinsic viscosity	1166:1184	Intrinsic viscosity	1166:1184	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	6	113	from	bonding	1030:1036	arg1	sites					1112:1116	conformationally well-defined sites	1082:1116	conformationally well-defined sites on the polymer	1082:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	14	114	theme	pectate	2738:2744	arg1	conformation					2715:2726	the starting conformation	2702:2726	the starting conformation of sodium pectate in aqueous solution	2702:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	14	114	theme	pectate	2738:2744	arg1	"					2697:2697	a "loose-21-helix"	2680:2697	a "loose-21-helix"	2680:2697	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	6	115	with	bonding	1030:1036	arg1	annihilation					1152:1163	local charge annihilation	1139:1163	local charge annihilation	1139:1163	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	12	116	theme	calcium-to-pectate	2352:2369	arg1	association					2371:2381	calcium-to-pectate association	2352:2381	calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process	2352:2460	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
34730949	4	117	theme	ideal	427:431	arg1	galacturonate					442:454	galacturonate	442:454	galacturonate	442:454	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	117	theme	ideal	427:431	arg1	Na+-poly					433:440	ideal Na+-poly	427:440	ideal Na+-poly(galacturonate)	427:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	9	118	theme	conformational	1720:1733	arg1	investigation					1744:1756	conformational analysis investigation	1720:1756	conformational analysis investigation	1720:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	11	119	theme	energy	2054:2059	arg1	values					2098:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values	2034:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes	2034:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	6	120	theme	polyelectrolytes	839:854	arg1	instrument					750:759	The key instrument	742:759	The key instrument of interpretation	742:777	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	120	theme	polyelectrolytes	839:854	arg1	theory					816:821	the counterion condensation theory	788:821	the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"),	788:989	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	120	theme	polyelectrolytes	839:854	arg1	bonding					1030:1036	strong chemical bonding	1014:1036	strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer	1014:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	120	theme	polyelectrolytes	839:854	arg1	CCT					824:826	CCT	824:826	CCT	824:826	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	3	121	theme	calcium	309:315	arg1	ions					317:320	calcium ions	309:320	calcium ions	309:320	It is able to form strong ionotropic gels by the addition of ions, in particular, calcium ions.					
34730949	9	122	theme	investigation	1744:1756	arg1	basis					1711:1715	the basis	1707:1715	the basis of conformational analysis investigation	1707:1756	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	14	123	theme	starting	2706:2713	arg1	conformation					2715:2726	the starting conformation	2702:2726	the starting conformation of sodium pectate in aqueous solution	2702:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	14	123	theme	starting	2706:2713	arg1	"					2697:2697	a "loose-21-helix"	2680:2697	a "loose-21-helix"	2680:2697	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	6	124	theme	counterion	932:941	arg1	affinity					907:914	both specific affinity	893:914	both specific affinity of the divalent counterion for the polysaccharide ("territorial binding")	893:988	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	9	125	theme	egg-box	1557:1563	arg1	type					1566:1569	the "tilted egg-box" type	1545:1569	the "tilted egg-box" type	1545:1569	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	4	126	theme	molar	636:640	arg1	concentrations					642:655	the molar concentrations	632:655	the molar concentrations of Ca2+ and pectate repeating units	632:691	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	4	127	with	pectate	384:390	arg1	composition					399:409	a composition	397:409	a composition close to 90% of ideal Na+-poly(galacturonate)	397:455	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	9	128	dep	development	1463:1473	arg1	order					1454:1458	order	1454:1458	order	1454:1458	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	9	128	dep	development	1463:1473	arg1	the					1450:1452	the	1450:1452	the	1450:1452	In the order of development as a function of Rj, the first mode (type 1) could be reconciled with the "tilted egg-box" type, recently proposed for Ca2+ binding to alginate and the second mode (type 2) with the "shifted egg-box" proposed for calcium pectate on the basis of conformational analysis investigation.					
34730949	14	129	theme	comparative	2591:2601	arg1	analysis					2603:2610	comparative analysis	2591:2610	comparative analysis of different techniques, but of CD	2591:2645	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	11	130	theme	entropy	2072:2078	arg1	values					2098:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values	2034:2103	standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes	2034:2160	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	14	131	theme	loose-21-helix	2683:2696	arg1	conformation					2715:2726	the starting conformation	2702:2726	the starting conformation of sodium pectate in aqueous solution	2702:2764	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	14	131	theme	loose-21-helix	2683:2696	arg1	"					2697:2697	a "loose-21-helix"	2680:2697	a "loose-21-helix"	2680:2697	Finally, comparative analysis of different techniques, but of CD in particular, enabled proposing a "loose-21-helix" as the starting conformation of sodium pectate in aqueous solution.					
34730949	6	132	theme	affinity	907:914	arg1	presence					881:888	the presence	877:888	the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding")	877:988	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	8	133	theme	curve	1387:1391	arg1	analysis					1353:1360	The analysis	1349:1360	The analysis of the microcalorimetric curve using the modified CCT	1349:1414	The analysis of the microcalorimetric curve using the modified CCT revealed two types of bonding.					
34730949	6	134	theme	chemical	1021:1028	arg1	bonding					1030:1036	strong chemical bonding	1014:1036	strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer	1014:1131	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	4	135	theme	repeating	677:685	arg1	units					687:691	pectate repeating units	669:691	pectate repeating units	669:691	The initial steps of binding Ca2+ ions to a sample of sodium pectate with a composition close to 90% of ideal Na+-poly(galacturonate) were investigated by means of circular dichroism (CD), microcalorimetry, dilatometry, viscosity, and membrane osmometry, as a function of increasing Rj, Rj being the ratio of the molar concentrations of Ca2+ and pectate repeating units.					
34730949	0	136	theme	Gelation	50:57	arg1	Mechanism					17:25	the Molecular Mechanism	3:25	the Molecular Mechanism of the Calcium-Induced Gelation of Pectate	3:68	On the Molecular Mechanism of the Calcium-Induced Gelation of Pectate.					
34730949	2	137	theme	hydrocolloid	187:198	arg1	one					160:162	one	160:162	one	160:162	Pectic acid/sodium pectate is one of the most widespread hydrocolloid used in the food industry.					
34730949	2	137	theme	hydrocolloid	187:198	arg1	hydrocolloid					187:198	the most widespread hydrocolloid	167:198	the most widespread hydrocolloid used in the food industry	167:224	Pectic acid/sodium pectate is one of the most widespread hydrocolloid used in the food industry.					
34730949	6	138	theme	territorial	968:978	arg1	polysaccharide					951:964	the polysaccharide	947:964	the polysaccharide ("territorial binding")	947:988	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	6	138	theme	territorial	968:978	arg1	"					987:987	"territorial binding"	967:987	"territorial binding"	967:987	The key instrument of interpretation has been the counterion condensation theory (CCT) of linear polyelectrolytes, modified to include the presence of both specific affinity of the divalent counterion for the polysaccharide ("territorial binding"), and, very importantly, strong chemical bonding (not a covalent bonding, though) of Ca2+ on conformationally well-defined sites on the polymer, with local charge annihilation.					
34730949	10	139	theme	bonding	1786:1792	arg1	types					1777:1781	the two types	1769:1781	the two types of bonding	1769:1792	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	10	139	theme	bonding	1786:1792	arg1	superimposable					1811:1824	superimposable	1811:1824	superimposable	1811:1824	Likewise, the two types of bonding turned out to be superimposable with similar bonding categories proposed for alginate and low-methoxyl pectin (LMP), on the one side, and for the association of semiflexible polyelectrolytes, on the other.					
34730949	1	140	from	Steps	81:85	arg1	Binding					94:100	the Binding	90:100	the Binding of Calcium Ions by Pectate	90:127	Different Steps in the Binding of Calcium Ions by Pectate.					
34730949	7	141	from	beginning	1315:1323	arg1	right					1300:1304	right	1300:1304	right	1300:1304	Intrinsic viscosity and number-average molar mass data as a function of Rj showed that calcium bonding brings about chain association right from the beginning of addition to pectate.					
34730949	11	142	theme	Gibbs	2043:2047	arg1	energy					2054:2059	standard Gibbs free energy	2034:2059	standard Gibbs free energy	2034:2059	The analysis allowed us to obtain standard Gibbs free energy, enthalpy, entropy, and volume molar values both for the affinity and the chemical bonding processes.					
34730949	12	143	theme	temperatures	2225:2236	arg1	dependence					2198:2207	the dependence	2194:2207	the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions	2194:2290	Interestingly, the analysis of the dependence of the gelation temperatures, Tg, of LMP upon increasing additions of calcium ions provided the values of Tg and standard Gibbs free-energy of calcium-to-pectate association coinciding with those obtained from calorimetry for the type-2 bonding process.					
33255096	6	0	theme	infrared	1036:1043	arg1	spectroscopy					1045:1056	infrared spectroscopy	1036:1056	infrared spectroscopy	1036:1056	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	10	1	theme	diluted	1802:1808	arg1	species					1810:1816	diluted species	1802:1816	diluted species	1802:1816	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	12	2	from	pain	2301:2304	arg1	cattle					2309:2314	cattle	2309:2314	cattle	2309:2314	These results demonstrate that chitosan/meloxicam microneedles patches may be suitable to manage pain in cattle after routine procedures.					
33255096	1	3	theme	patch	235:239	arg1	evaluation					198:207	evaluation	198:207	evaluation	198:207	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
33255096	1	3	theme	patch	235:239	arg1	formulation					182:192	formulation	182:192	formulation	182:192	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
33255096	6	4	theme	amide	1188:1192	arg1	I					1194:1194	amide I	1188:1194	amide I	1188:1194	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	3	5	theme	Microneedle	553:563	arg1	patches					565:571	Microneedle patches	553:571	Microneedle patches	553:571	Microneedle patches were prepared by varying the percentage of acetic acid used during solution preparation, including 90% (v/v), 50% (v/v), and 10% (v/v).					
33255096	8	6	theme	penetrated	1547:1556	arg1	skin					1558:1561	the penetrated skin	1543:1561	the penetrated skin	1543:1561	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	6	7	theme	chitosan	1178:1185	arg1	bands					1169:1173	the characteristic bands	1150:1173	the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch)	1150:1256	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	4	8	theme	penetration	785:795	arg1	percentages					770:780	different percentages	760:780	different percentages of penetration into the skin	760:809	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	4	8	theme	penetration	785:795	arg1	number					819:824	the number	815:824	the number of microneedles on the microneedle patch	815:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	6	9	theme	acetic	1085:1090	arg1	acid					1092:1095	acetic acid	1085:1095	acetic acid in the solution	1085:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	11	10	theme	pain	2142:2145	arg1	management					2147:2156	pain management	2142:2156	pain management in cattle, established as 1.43 × 10-4 mol/m3	2142:2201	One patch released a drug concentration of 3.57 × 10-5 mol/m3 in the skin per week, which represents the 26.2% of what is needed for pain management in cattle, established as 1.43 × 10-4 mol/m3.					
33255096	6	11	dep	meloxicam	1214:1222	arg1	stretch					1249:1255	CO stretch	1246:1255	CO stretch	1246:1255	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	11	dep	meloxicam	1214:1222	arg1	stretch					1234:1240	amine NH stretch	1225:1240	amine NH stretch	1225:1240	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	10	12	theme	drug	1858:1861	arg1	delivery					1863:1870	the transdermal drug delivery	1842:1870	the transdermal drug delivery	1842:1870	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	6	13	theme	NH	1231:1232	arg1	stretch					1234:1240	amine NH stretch	1225:1240	amine NH stretch	1225:1240	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	5	14	theme	acid	1007:1010	arg1	percentage					986:995	each percentage	981:995	each percentage of acetic acid used	981:1015	Scanning electron microscopy confirmed the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used.					
33255096	6	15	theme	characteristic	1154:1167	arg1	bands					1169:1173	the characteristic bands	1150:1173	the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch)	1150:1256	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	0	16	theme	pain	114:117	arg1	patch					65:69	patch	65:69	patch for transdermal delivery of meloxicam	65:107	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	0	16	theme	pain	114:117	arg1	strategy					130:137	a pain management strategy	112:137	a pain management strategy for use in cattle	112:155	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	11	17	theme	3.57 × 10-5 mol/m3	2052:2069	arg1	concentration					2035:2047	a drug concentration	2028:2047	a drug concentration of 3.57 × 10-5 mol/m3 in the skin	2028:2081	One patch released a drug concentration of 3.57 × 10-5 mol/m3 in the skin per week, which represents the 26.2% of what is needed for pain management in cattle, established as 1.43 × 10-4 mol/m3.					
33255096	12	18	theme	chitosan/meloxicam	2235:2252	arg1	patches					2267:2273	chitosan/meloxicam microneedles patches	2235:2273	chitosan/meloxicam microneedles patches	2235:2273	These results demonstrate that chitosan/meloxicam microneedles patches may be suitable to manage pain in cattle after routine procedures.					
33255096	4	19	from	microneedles	829:840	arg1	patch					861:865	the microneedle patch	845:865	the microneedle patch	845:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	7	20	theme	cadaver	1435:1441	arg1	ear					1443:1445	a cow's cadaver ear	1427:1445	a cow's cadaver ear	1427:1445	The resultant microneedle patches were successful in penetrating the skin in a cow's cadaver ear.					
33255096	4	21	theme	microneedles	829:840	arg1	percentages					770:780	different percentages	760:780	different percentages of penetration into the skin	760:809	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	4	21	theme	microneedles	829:840	arg1	number					819:824	the number	815:824	the number of microneedles on the microneedle patch	815:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	10	22	theme	number	1918:1923	arg1	increment					1901:1909	the increment	1897:1909	the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle	1897:2006	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	4	23	from	number	819:824	arg1	patch					861:865	the microneedle patch	845:865	the microneedle patch	845:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	9	24	with	patches	1636:1642	arg1	acid					1663:1666	higher acetic acid	1649:1666	higher acetic acid	1649:1666	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	0	25	theme	chitosan	44:51	arg1	characterization					8:23	characterization	8:23	characterization	8:23	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	0	25	theme	chitosan	44:51	arg1	modeling					30:37	modeling	30:37	modeling	30:37	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	0	25	theme	chitosan	44:51	arg1	Design					0:5	Design	0:5	Design	0:5	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	3	26	theme	acid	623:626	arg1	percentage					602:611	the percentage	598:611	the percentage of acetic acid used during solution preparation, including 90% (v/v), 50% (v/v), and 10% (v/v)	598:706	Microneedle patches were prepared by varying the percentage of acetic acid used during solution preparation, including 90% (v/v), 50% (v/v), and 10% (v/v).					
33255096	9	27	theme	microneedle	1624:1634	arg1	patches					1636:1642	chitosan/meloxicam microneedle patches	1605:1642	chitosan/meloxicam microneedle patches with higher acetic acid	1605:1666	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	10	28	from	patch	1956:1960	arg1	percentage					1966:1975	percentage	1966:1975	percentage of penetration per microneedle	1966:2006	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	10	28	from	patch	1956:1960	arg1	number					1918:1923	the number	1914:1923	the number of microneedles on the surface patch	1914:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	10	29	theme	microneedles	1928:1939	arg1	percentage					1966:1975	percentage	1966:1975	percentage of penetration per microneedle	1966:2006	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	10	29	theme	microneedles	1928:1939	arg1	number					1918:1923	the number	1914:1923	the number of microneedles on the surface patch	1914:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	9	30	dep	Chitosan	1592:1599	arg1	percentages					1668:1678	percentages	1668:1678	percentages	1668:1678	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	9	31	theme	acetic	1656:1661	arg1	acid					1663:1666	higher acetic acid	1649:1666	higher acetic acid	1649:1666	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	10	32	from	percentage	1966:1975	arg1	patch					1956:1960	the surface patch	1944:1960	the surface patch	1944:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	6	33	from	acid	1092:1095	arg1	solution					1104:1111	the solution	1100:1111	the solution	1100:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	4	34	from	percentages	770:780	arg1	patch					861:865	the microneedle patch	845:865	the microneedle patch	845:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	2	35	theme	characteristics	449:463	arg1	capacity					466:473	capacity	466:473	capacity to penetrate the skin	466:495	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	2	35	theme	characteristics	449:463	arg1	uniformity					426:435	uniformity	426:435	uniformity of physical characteristics	426:463	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	2	35	theme	characteristics	449:463	arg1	composition					413:423	their chemical composition	398:423	their chemical composition	398:423	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	2	35	theme	characteristics	449:463	arg1	response					502:509	response	502:509	response to thermal and thermo-mechanical changes	502:550	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	1	36	theme	transdermal	249:259	arg1	delivery					261:268	the transdermal delivery	245:268	the transdermal delivery of meloxicam to manage pain in cattle	245:306	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
33255096	5	37	theme	Scanning	868:875	arg1	microscopy					886:895	Scanning electron microscopy	868:895	Scanning electron microscopy	868:895	Scanning electron microscopy confirmed the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used.					
33255096	9	38	theme	greater	1690:1696	arg1	resistance					1698:1707	greater resistance	1690:1707	greater resistance to compressive force	1690:1728	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	11	39	from	management	2147:2156	arg1	cattle					2161:2166	cattle	2161:2166	cattle	2161:2166	One patch released a drug concentration of 3.57 × 10-5 mol/m3 in the skin per week, which represents the 26.2% of what is needed for pain management in cattle, established as 1.43 × 10-4 mol/m3.					
33255096	6	40	dep	Fourier	1018:1024	arg1	transform					1026:1034	transform	1026:1034	transform infrared spectroscopy	1026:1056	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	2	41	theme	chemical	404:411	arg1	composition					413:423	their chemical composition	398:423	their chemical composition	398:423	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	7	42	theme	resultant	1354:1362	arg1	successful					1389:1398	successful	1389:1398	successful	1389:1398	The resultant microneedle patches were successful in penetrating the skin in a cow's cadaver ear.					
33255096	7	42	theme	resultant	1354:1362	arg1	patches					1376:1382	The resultant microneedle patches	1350:1382	The resultant microneedle patches	1350:1382	The resultant microneedle patches were successful in penetrating the skin in a cow's cadaver ear.					
33255096	5	43	theme	patch	963:967	arg1	surface					969:975	the patch surface	959:975	the patch surface	959:975	Scanning electron microscopy confirmed the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used.					
33255096	2	44	theme	thermal	514:520	arg1	changes					544:550	thermal and thermo-mechanical changes	514:550	thermal and thermo-mechanical changes	514:550	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	10	45	theme	percentage	1966:1975	arg1	increment					1901:1909	the increment	1897:1909	the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle	1897:2006	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	12	46	theme	routine	2322:2328	arg1	procedures					2330:2339	routine procedures	2322:2339	routine procedures	2322:2339	These results demonstrate that chitosan/meloxicam microneedles patches may be suitable to manage pain in cattle after routine procedures.					
33255096	1	47	dep	formulation	182:192	arg1	the					178:180	the	178:180	the	178:180	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
33255096	6	48	from	%	1074:1074	arg1	solution					1104:1111	the solution	1100:1111	the solution	1100:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	8	49	theme	average	1478:1484	arg1	penetration					1492:1502	the average depth penetration	1474:1502	the average depth penetration measured after complete dehydration of the penetrated skin	1474:1561	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	8	49	theme	average	1478:1484	arg1	78 ± 1 μm					1581:1589	78 ± 1 μm	1581:1589	78 ± 1 μm	1581:1589	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	6	50	theme	acid	1307:1310	arg1	v/v					1278:1280	v/v	1278:1280	v/v	1278:1280	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	50	theme	acid	1307:1310	arg1	%					1275:1275	90%	1273:1275	90% (v/v)	1273:1281	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	50	theme	acid	1307:1310	arg1	%					1289:1289	50%	1287:1289	50% (v/v)	1287:1295	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	50	theme	acid	1307:1310	arg1	v/v					1292:1294	v/v	1292:1294	v/v	1292:1294	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	50	theme	acid	1307:1310	arg1	acid					1307:1310	acetic acid	1300:1310	acetic acid used during the solution preparation	1300:1347	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	10	51	theme	species	1810:1816	arg1	transport					1789:1797	the transport	1785:1797	the transport of diluted species	1785:1816	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	6	52	theme	amide	1200:1204	arg1	II					1206:1207	amide II	1200:1207	amide II	1200:1207	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	10	53	from	microneedles	1928:1939	arg1	patch					1956:1960	the surface patch	1944:1960	the surface patch	1944:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	1	54	theme	microneedle	223:233	arg1	patch					235:239	a chitosan microneedle patch	212:239	a chitosan microneedle patch	212:239	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
33255096	6	55	dep	chitosan	1178:1185	arg1	I					1194:1194	amide I	1188:1194	amide I	1188:1194	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	55	dep	chitosan	1178:1185	arg1	II					1206:1207	amide II	1200:1207	amide II	1200:1207	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	10	56	theme	penetration	1980:1990	arg1	percentage					1966:1975	percentage	1966:1975	percentage of penetration per microneedle	1966:2006	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	10	56	theme	penetration	1980:1990	arg1	number					1918:1923	the number	1914:1923	the number of microneedles on the surface patch	1914:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	0	57	from	use	143:145	arg1	cattle					150:155	cattle	150:155	cattle	150:155	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	4	58	theme	different	760:768	arg1	percentages					770:780	different percentages	760:780	different percentages of penetration into the skin	760:809	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	6	59	theme	acid	1092:1095	arg1	v/v					1077:1079	v/v	1077:1079	v/v	1077:1079	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	59	theme	acid	1092:1095	arg1	condition					1128:1136	a suitable condition	1117:1136	a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation	1117:1347	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	59	theme	acid	1092:1095	arg1	%					1074:1074	10%	1072:1074	10% (v/v) of acetic acid in the solution	1072:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	59	theme	acid	1092:1095	arg1	acid					1092:1095	acetic acid	1085:1095	acetic acid in the solution	1085:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	8	60	theme	skin	1558:1561	arg1	dehydration					1528:1538	complete dehydration	1519:1538	complete dehydration of the penetrated skin	1519:1561	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	11	61	theme	drug	2030:2033	arg1	concentration					2035:2047	a drug concentration	2028:2047	a drug concentration of 3.57 × 10-5 mol/m3 in the skin	2028:2081	One patch released a drug concentration of 3.57 × 10-5 mol/m3 in the skin per week, which represents the 26.2% of what is needed for pain management in cattle, established as 1.43 × 10-4 mol/m3.					
33255096	10	62	theme	transdermal	1846:1856	arg1	delivery					1863:1870	the transdermal drug delivery	1842:1870	the transdermal drug delivery	1842:1870	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	0	63	theme	management	119:128	arg1	patch					65:69	patch	65:69	patch for transdermal delivery of meloxicam	65:107	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	0	63	theme	management	119:128	arg1	strategy					130:137	a pain management strategy	112:137	a pain management strategy for use in cattle	112:155	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	6	64	theme	solution	1328:1335	arg1	preparation					1337:1347	the solution preparation	1324:1347	the solution preparation	1324:1347	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	4	65	theme	microneedle	849:859	arg1	patch					861:865	the microneedle patch	845:865	the microneedle patch	845:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	6	66	theme	amine	1225:1229	arg1	stretch					1234:1240	amine NH stretch	1225:1240	amine NH stretch	1225:1240	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	67	theme	meloxicam	1214:1222	arg1	bands					1169:1173	the characteristic bands	1150:1173	the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch)	1150:1256	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	68	theme	suitable	1119:1126	arg1	condition					1128:1136	a suitable condition	1117:1136	a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation	1117:1347	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	68	theme	suitable	1119:1126	arg1	%					1074:1074	10%	1072:1074	10% (v/v) of acetic acid in the solution	1072:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	68	theme	suitable	1119:1126	arg1	acid					1092:1095	acetic acid	1085:1095	acetic acid in the solution	1085:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	5	69	theme	acetic	1000:1005	arg1	acid					1007:1010	acetic acid	1000:1010	acetic acid used	1000:1015	Scanning electron microscopy confirmed the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used.					
33255096	3	70	theme	acetic	616:621	arg1	acid					623:626	acetic acid	616:626	acetic acid used during solution preparation, including 90% (v/v), 50% (v/v), and 10% (v/v)	616:706	Microneedle patches were prepared by varying the percentage of acetic acid used during solution preparation, including 90% (v/v), 50% (v/v), and 10% (v/v).					
33255096	9	71	theme	chitosan/meloxicam	1605:1622	arg1	patches					1636:1642	chitosan/meloxicam microneedle patches	1605:1642	chitosan/meloxicam microneedle patches with higher acetic acid	1605:1666	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	0	72	theme	transdermal	75:85	arg1	delivery					87:94	transdermal delivery	75:94	transdermal delivery of meloxicam	75:107	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	11	73	from	concentration	2035:2047	arg1	skin					2078:2081	the skin	2074:2081	the skin	2074:2081	One patch released a drug concentration of 3.57 × 10-5 mol/m3 in the skin per week, which represents the 26.2% of what is needed for pain management in cattle, established as 1.43 × 10-4 mol/m3.					
33255096	4	74	from	patch	861:865	arg1	percentages					770:780	different percentages	760:780	different percentages of penetration into the skin	760:809	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	4	74	from	patch	861:865	arg1	number					819:824	the number	815:824	the number of microneedles on the microneedle patch	815:865	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	9	75	theme	higher	1649:1654	arg1	acid					1663:1666	higher acetic acid	1649:1666	higher acetic acid	1649:1666	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	0	76	theme	meloxicam	99:107	arg1	delivery					87:94	transdermal delivery	75:94	transdermal delivery of meloxicam	75:107	Design, characterization, and modeling of a chitosan microneedle patch for transdermal delivery of meloxicam as a pain management strategy for use in cattle.					
33255096	6	77	from	solution	1104:1111	arg1	v/v					1077:1079	v/v	1077:1079	v/v	1077:1079	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	77	from	solution	1104:1111	arg1	condition					1128:1136	a suitable condition	1117:1136	a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation	1117:1347	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	77	from	solution	1104:1111	arg1	%					1074:1074	10%	1072:1074	10% (v/v) of acetic acid in the solution	1072:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	77	from	solution	1104:1111	arg1	acid					1092:1095	acetic acid	1085:1095	acetic acid in the solution	1085:1111	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	6	78	theme	CO	1246:1247	arg1	stretch					1249:1255	CO stretch	1246:1255	CO stretch	1246:1255	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	2	79	theme	physical	440:447	arg1	characteristics					449:463	physical characteristics	440:463	physical characteristics	440:463	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	5	80	theme	electron	877:884	arg1	microscopy					886:895	Scanning electron microscopy	868:895	Scanning electron microscopy	868:895	Scanning electron microscopy confirmed the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used.					
33255096	10	81	theme	Time-dependent	1756:1769	arg1	simulation					1771:1780	Time-dependent simulation	1756:1780	Time-dependent simulation of the transport of diluted species by COMSOL	1756:1826	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	1	82	theme	meloxicam	273:281	arg1	delivery					261:268	the transdermal delivery	245:268	the transdermal delivery of meloxicam to manage pain in cattle	245:306	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
33255096	10	83	from	number	1918:1923	arg1	patch					1956:1960	the surface patch	1944:1960	the surface patch	1944:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	9	84	theme	compressive	1712:1722	arg1	force					1724:1728	compressive force	1712:1728	compressive force	1712:1728	Chitosan and chitosan/meloxicam microneedle patches with higher acetic acid percentages reflected greater resistance to compressive force as temperature increased.					
33255096	3	85	theme	solution	640:647	arg1	preparation					649:659	solution preparation	640:659	solution preparation	640:659	Microneedle patches were prepared by varying the percentage of acetic acid used during solution preparation, including 90% (v/v), 50% (v/v), and 10% (v/v).					
33255096	7	86	from	skin	1419:1422	arg1	ear					1443:1445	a cow's cadaver ear	1427:1445	a cow's cadaver ear	1427:1445	The resultant microneedle patches were successful in penetrating the skin in a cow's cadaver ear.					
33255096	7	87	theme	microneedle	1364:1374	arg1	successful					1389:1398	successful	1389:1398	successful	1389:1398	The resultant microneedle patches were successful in penetrating the skin in a cow's cadaver ear.					
33255096	7	87	theme	microneedle	1364:1374	arg1	patches					1376:1382	The resultant microneedle patches	1350:1382	The resultant microneedle patches	1350:1382	The resultant microneedle patches were successful in penetrating the skin in a cow's cadaver ear.					
33255096	2	88	theme	thermo-mechanical	526:542	arg1	changes					544:550	thermal and thermo-mechanical changes	514:550	thermal and thermo-mechanical changes	514:550	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	10	89	theme	transport	1789:1797	arg1	simulation					1771:1780	Time-dependent simulation	1756:1780	Time-dependent simulation of the transport of diluted species by COMSOL	1756:1826	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	12	90	theme	microneedles	2254:2265	arg1	patches					2267:2273	chitosan/meloxicam microneedles patches	2235:2273	chitosan/meloxicam microneedles patches	2235:2273	These results demonstrate that chitosan/meloxicam microneedles patches may be suitable to manage pain in cattle after routine procedures.					
33255096	2	91	theme	Microneedle	309:319	arg1	patches					321:327	Microneedle patches	309:327	Microneedle patches composed of chitosan and chitosan/meloxicam	309:371	Microneedle patches composed of chitosan and chitosan/meloxicam were evaluated regarding their chemical composition, uniformity of physical characteristics, capacity to penetrate the skin, and response to thermal and thermo-mechanical changes.					
33255096	8	92	theme	depth	1486:1490	arg1	penetration					1492:1502	the average depth penetration	1474:1502	the average depth penetration measured after complete dehydration of the penetrated skin	1474:1561	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	8	92	theme	depth	1486:1490	arg1	78 ± 1 μm					1581:1589	78 ± 1 μm	1581:1589	78 ± 1 μm	1581:1589	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	5	93	theme	microneedles	923:934	arg1	presence					911:918	the presence	907:918	the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used	907:1015	Scanning electron microscopy confirmed the presence of microneedles uniformly organized on the patch surface for each percentage of acetic acid used.					
33255096	6	94	theme	acetic	1300:1305	arg1	acid					1307:1310	acetic acid	1300:1310	acetic acid used during the solution preparation	1300:1347	Fourier transform infrared spectroscopy revealed that 10% (v/v) of acetic acid in the solution was a suitable condition to preserve the characteristic bands of chitosan (amide I and amide II) and meloxicam (amine NH stretch and CO stretch) as compared to 90% (v/v) and 50% (v/v) of acetic acid used during the solution preparation.					
33255096	10	95	theme	surface	1948:1954	arg1	patch					1956:1960	the surface patch	1944:1960	the surface patch	1944:1960	Time-dependent simulation of the transport of diluted species by COMSOL revealed that the transdermal drug delivery increases in function to the increment of the number of microneedles on the surface patch and percentage of penetration per microneedle.					
33255096	4	96	theme	drug	722:725	arg1	release					727:733	drug release	722:733	drug release	722:733	In addition, drug release was assessed by modeling different percentages of penetration into the skin and the number of microneedles on the microneedle patch.					
33255096	8	97	theme	complete	1519:1526	arg1	dehydration					1528:1538	complete dehydration	1519:1538	complete dehydration of the penetrated skin	1519:1561	Results demonstrated that the average depth penetration measured after complete dehydration of the penetrated skin was approximately 78 ± 1 μm.					
33255096	1	98	theme	chitosan	214:221	arg1	patch					235:239	a chitosan microneedle patch	212:239	a chitosan microneedle patch	212:239	This work describes the formulation and evaluation of a chitosan microneedle patch for the transdermal delivery of meloxicam to manage pain in cattle.					
32827615	3	0	theme	activated	469:477	arg1	ABn-AG					499:504	ABn-AG	499:504	ABn-AG	499:504	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	3	0	theme	activated	469:477	arg1	beads					517:521	activated bentonite-alginate (ABn-AG) composite beads	469:521	activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	469:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	6	1	theme	%	1148:1148	arg1	%					1148:1148	Cu2+and 77%	1138:1148	Cu2+and 77% of Pb2+were removed at pH 2.0	1138:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	6	1	theme	%	1148:1148	arg1	%					1133:1133	58%	1131:1133	58% of Cu2+and 77% of Pb2+were removed at pH 2.0	1131:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	6	1	theme	%	1148:1148	arg1	Pb2+were					1153:1160	Pb2+were	1153:1160	Pb2+were removed at pH 2.0	1153:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	11	2	theme	Pb2+	1739:1742	arg1	removal					1719:1725	removal	1719:1725	removal of Cu2+ and Pb2+	1719:1742	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	9	3	theme	Cu2+	1545:1548	arg1	removal					1534:1540	the removal	1530:1540	the removal of Cu2+ and Pb2+ using ABn-AG	1530:1570	The presence of MgCl2, NaCl and KCl did not cause significant influence on the removal of Cu2+ and Pb2+ using ABn-AG.					
32827615	7	4	theme	order	1326:1330	arg1	model					1340:1344	the pseudo-second order kinetic model	1308:1344	the pseudo-second order kinetic model	1308:1344	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	4	5	theme	ionic	637:641	arg1	method					652:657	the ionic gelation method	633:657	the ionic gelation method	633:657	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	11	6	theme	robust	1848:1853	arg1	material					1855:1862	a highly robust material	1839:1862	a highly robust material	1839:1862	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	11	6	theme	robust	1848:1853	arg1	ABn-AG					1829:1834	ABn-AG	1829:1834	ABn-AG	1829:1834	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	1	7	theme	water	168:172	arg1	bodies					174:179	water bodies	168:179	water bodies	168:179	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	9	8	theme	Pb2+	1554:1557	arg1	removal					1534:1540	the removal	1530:1540	the removal of Cu2+ and Pb2+ using ABn-AG	1530:1570	The presence of MgCl2, NaCl and KCl did not cause significant influence on the removal of Cu2+ and Pb2+ using ABn-AG.					
32827615	6	9	theme	acidic	1106:1111	arg1	conditions					1113:1122	highly acidic conditions	1099:1122	highly acidic conditions	1099:1122	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	2	10	theme	present	277:283	arg1	metals					270:275	the two common toxic metals	249:275	the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications	249:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	10	theme	present	277:283	arg1	lead					240:243	lead	240:243	lead	240:243	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	10	theme	present	277:283	arg1	Copper					229:234	Copper	229:234	Copper	229:234	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	11	from	usage	332:336	arg1	industries					349:358	various industries	341:358	various industries	341:358	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	0	12	theme	aquatic	107:113	arg1	environment					115:125	aquatic environment	107:125	aquatic environment	107:125	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	2	13	theme	toxic	264:268	arg1	metals					270:275	the two common toxic metals	249:275	the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications	249:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	13	theme	toxic	264:268	arg1	lead					240:243	lead	240:243	lead	240:243	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	13	theme	toxic	264:268	arg1	Copper					229:234	Copper	229:234	Copper	229:234	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	11	14	theme	several	1885:1891	arg1	times					1893:1897	several times	1885:1897	several times	1885:1897	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	6	15	theme	Pb2+were	1153:1160	arg1	%					1148:1148	Cu2+and 77%	1138:1148	Cu2+and 77% of Pb2+were removed at pH 2.0	1138:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	6	15	theme	Pb2+were	1153:1160	arg1	Pb2+were					1153:1160	Pb2+were	1153:1160	Pb2+were removed at pH 2.0	1153:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	5	16	with	isotherm	905:912	arg1	loops					949:953	characteristics H3 hysteresis loops	919:953	characteristics H3 hysteresis loops	919:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	3	17	theme	adsorption	570:579	arg1	reaction					581:588	adsorption reaction	570:588	adsorption reaction	570:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	1	18	theme	major	195:199	arg1	one					184:186	one	184:186	one	184:186	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	1	18	theme	major	195:199	arg1	major					195:199	major	195:199	major	195:199	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	8	19	theme	sorption	1359:1366	arg1	17.30 mg/g					1402:1411	17.30 mg/g	1402:1411	17.30 mg/g	1402:1411	The maximum sorption capacity for Cu2+ was found to be 17.30 mg/g whereas Pb2+ was found to be 107.52 mg/g.					
32827615	8	19	theme	sorption	1359:1366	arg1	capacity					1368:1375	The maximum sorption capacity	1347:1375	The maximum sorption capacity for Cu2+	1347:1384	The maximum sorption capacity for Cu2+ was found to be 17.30 mg/g whereas Pb2+ was found to be 107.52 mg/g.					
32827615	7	20	theme	contact	1278:1284	arg1	time					1286:1289	various contact time	1270:1289	various contact time	1270:1289	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	6	21	dep	%	1133:1133	arg1	i.e.					1125:1128	i.e.	1125:1128	i.e.	1125:1128	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	11	22	theme	Reusability	1690:1700	arg1	test					1702:1705	Reusability test	1690:1705	Reusability test	1690:1705	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	1	23	from	countries	218:226	arg1	major					195:199	major	195:199	major	195:199	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	10	24	theme	present	1671:1677	arg1	sites					1665:1669	different binding sites	1647:1669	different binding sites present in ABn-AG	1647:1687	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	5	25	theme	adsorption-desorption	830:850	arg1	isotherm					852:859	The nitrogen adsorption-desorption isotherm	817:859	The nitrogen adsorption-desorption isotherm obtained for the materials	817:886	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	5	25	theme	adsorption-desorption	830:850	arg1	isotherm					905:912	the type IV isotherm	893:912	the type IV isotherm with characteristics H3 hysteresis loops	893:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	6	26	theme	Cu2+and	1138:1144	arg1	%					1148:1148	Cu2+and 77%	1138:1148	Cu2+and 77% of Pb2+were removed at pH 2.0	1138:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	6	26	theme	Cu2+and	1138:1144	arg1	Pb2+were					1153:1160	Pb2+were	1153:1160	Pb2+were removed at pH 2.0	1153:1178	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	3	27	theme	composite	507:515	arg1	ABn-AG					499:504	ABn-AG	499:504	ABn-AG	499:504	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	3	27	theme	composite	507:515	arg1	beads					517:521	activated bentonite-alginate (ABn-AG) composite beads	469:521	activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	469:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	1	28	theme	heavy	138:142	arg1	metals					144:149	The toxic heavy metals	128:149	The toxic heavy metals contamination in water bodies	128:179	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	0	29	theme	toxic	77:81	arg1	Cu2+					83:86	toxic Cu2+ and Pb2+ ions	77:100	Cu2+	83:86	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	4	30	theme	bentonite	715:723	arg1	ABn					726:728	activated bentonite (ABn)	705:729	activated bentonite (ABn)	705:729	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	5	31	theme	type	897:900	arg1	isotherm					852:859	The nitrogen adsorption-desorption isotherm	817:859	The nitrogen adsorption-desorption isotherm obtained for the materials	817:886	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	5	31	theme	type	897:900	arg1	isotherm					905:912	the type IV isotherm	893:912	the type IV isotherm with characteristics H3 hysteresis loops	893:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	12	32	theme	aqueous	2065:2071	arg1	waste					2073:2077	aqueous waste	2065:2077	aqueous waste	2065:2077	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	2	33	theme	diverse	364:370	arg1	applications					372:383	diverse applications	364:383	diverse applications	364:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	0	34	theme	ions	97:100	arg1	removal					66:72	efficient removal	56:72	efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment	56:125	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	7	35	theme	adsorption	1242:1251	arg1	data					1253:1256	the adsorption data	1238:1256	the adsorption data obtained at various contact time	1238:1289	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	5	36	with	mesopores	982:990	arg1	pores					1009:1013	slit-shaped pores	997:1013	slit-shaped pores	997:1013	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	4	37	theme	surface	773:779	arg1	area					781:784	BET surface area	769:784	BET surface area	769:784	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	4	37	theme	surface	773:779	arg1	XRD					764:766	XRD	764:766	XRD	764:766	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	12	38	theme	Cu2+	2046:2049	arg1	removal					2035:2041	the removal	2031:2041	the removal of Cu2+ and Pb2+ from aqueous waste	2031:2077	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	0	39	theme	activated	7:15	arg1	Use					0:2	Use	0:2	Use of activated	0:15	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	7	40	theme	contact	1221:1227	arg1	time					1229:1232	the initial contact time	1209:1232	the initial contact time	1209:1232	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	11	41	theme	same	1776:1779	arg1	material					1781:1788	the same material	1772:1788	the same material	1772:1788	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	0	42	theme	composite	36:44	arg1	beads					46:50	composite beads	36:50	composite beads	36:50	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	3	43	theme	metal	452:456	arg1	ions					458:461	these two toxic heavy metal ions	430:461	these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	430:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	3	44	theme	toxic	440:444	arg1	ions					458:461	these two toxic heavy metal ions	430:461	these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	430:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	5	45	theme	characteristics	919:933	arg1	loops					949:953	characteristics H3 hysteresis loops	919:953	characteristics H3 hysteresis loops	919:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	6	46	theme	percent	1062:1068	arg1	removal					1070:1076	reasonably high percent removal	1046:1076	reasonably high percent removal	1046:1076	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	10	47	theme	Binary	1573:1578	arg1	study					1591:1595	Binary adsorption study	1573:1595	Binary adsorption study	1573:1595	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	3	48	theme	bentonite-alginate	479:496	arg1	ABn-AG					499:504	ABn-AG	499:504	ABn-AG	499:504	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	3	48	theme	bentonite-alginate	479:496	arg1	beads					517:521	activated bentonite-alginate (ABn-AG) composite beads	469:521	activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	469:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	1	49	theme	many	213:216	arg1	countries					218:226	many countries	213:226	many countries	213:226	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	5	50	theme	hysteresis	938:947	arg1	loops					949:953	characteristics H3 hysteresis loops	919:953	characteristics H3 hysteresis loops	919:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	4	51	theme	Composite	591:599	arg1	beads					601:605	Composite beads	591:605	Composite beads	591:605	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	2	52	theme	extensive	322:330	arg1	usage					332:336	their extensive usage	316:336	their extensive usage in various industries for diverse applications	316:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	53	from	environments	296:307	arg1	present					277:283	present	277:283	present	277:283	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	54	theme	common	257:262	arg1	metals					270:275	the two common toxic metals	249:275	the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications	249:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	54	theme	common	257:262	arg1	lead					240:243	lead	240:243	lead	240:243	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	54	theme	common	257:262	arg1	Copper					229:234	Copper	229:234	Copper	229:234	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	10	55	theme	adsorption	1580:1589	arg1	study					1591:1595	Binary adsorption study	1573:1595	Binary adsorption study	1573:1595	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	4	56	theme	BET	769:771	arg1	area					781:784	BET surface area	769:784	BET surface area	769:784	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	4	56	theme	BET	769:771	arg1	XRD					764:766	XRD	764:766	XRD	764:766	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	7	57	theme	kinetic	1332:1338	arg1	model					1340:1344	the pseudo-second order kinetic model	1308:1344	the pseudo-second order kinetic model	1308:1344	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	0	58	from	removal	66:72	arg1	environment					115:125	aquatic environment	107:125	aquatic environment	107:125	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	2	59	attach	present	277:283	arg2	lead					240:243	lead	240:243	lead	240:243	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	59	attach	present	277:283	arg2	metals					270:275	the two common toxic metals	249:275	the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications	249:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	59	attach	present	277:283	arg1	environments					296:307	aquatic environments	288:307	aquatic environments due to their extensive usage in various industries for diverse applications	288:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	2	59	attach	present	277:283	arg2	Copper					229:234	Copper	229:234	Copper	229:234	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	7	60	theme	pseudo-second	1312:1324	arg1	model					1340:1344	the pseudo-second order kinetic model	1308:1344	the pseudo-second order kinetic model	1308:1344	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	4	61	theme	gelation	643:650	arg1	method					652:657	the ionic gelation method	633:657	the ionic gelation method	633:657	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	10	62	theme	different	1647:1655	arg1	sites					1665:1669	different binding sites	1647:1669	different binding sites present in ABn-AG	1647:1687	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	10	63	theme	binding	1657:1663	arg1	sites					1665:1669	different binding sites	1647:1669	different binding sites present in ABn-AG	1647:1687	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	8	64	theme	maximum	1351:1357	arg1	17.30 mg/g					1402:1411	17.30 mg/g	1402:1411	17.30 mg/g	1402:1411	The maximum sorption capacity for Cu2+ was found to be 17.30 mg/g whereas Pb2+ was found to be 107.52 mg/g.					
32827615	8	64	theme	maximum	1351:1357	arg1	capacity					1368:1375	The maximum sorption capacity	1347:1375	The maximum sorption capacity for Cu2+	1347:1384	The maximum sorption capacity for Cu2+ was found to be 17.30 mg/g whereas Pb2+ was found to be 107.52 mg/g.					
32827615	2	65	theme	due	309:311	arg1	environments					296:307	aquatic environments	288:307	aquatic environments due to their extensive usage in various industries for diverse applications	288:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	11	66	theme	Cu2+	1730:1733	arg1	removal					1719:1725	removal	1719:1725	removal of Cu2+ and Pb2+	1719:1742	Reusability test showed that removal of Cu2+ and Pb2+ decreased by 10% only after the same material was reused for 5 times indicating that ABn-AG is a highly robust material and can be reuse for several times without losing its efficiency.					
32827615	12	67	theme	composite	1969:1977	arg1	adsorbent					2017:2025	adsorbent	2017:2025	adsorbent	2017:2025	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	12	67	theme	composite	1969:1977	arg1	beads					1979:1983	ABn-AG composite beads	1962:1983	ABn-AG composite beads	1962:1983	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	10	68	attach	present	1671:1677	arg2	sites					1665:1669	different binding sites	1647:1669	different binding sites present in ABn-AG	1647:1687	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	10	68	attach	present	1671:1677	arg1	ABn-AG					1682:1687	ABn-AG	1682:1687	ABn-AG	1682:1687	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	1	69	from	major	195:199	arg1	countries					218:226	many countries	213:226	many countries	213:226	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	5	70	theme	nitrogen	821:828	arg1	isotherm					852:859	The nitrogen adsorption-desorption isotherm	817:859	The nitrogen adsorption-desorption isotherm obtained for the materials	817:886	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	5	70	theme	nitrogen	821:828	arg1	isotherm					905:912	the type IV isotherm	893:912	the type IV isotherm with characteristics H3 hysteresis loops	893:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	5	71	theme	mesopores	982:990	arg1	presence					970:977	the presence	966:977	the presence of mesopores with slit-shaped pores	966:1013	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	7	72	theme	various	1270:1276	arg1	time					1286:1289	various contact time	1270:1289	various contact time	1270:1289	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	4	73	theme	raw	684:686	arg1	BnR					699:701	BnR	699:701	BnR	699:701	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	4	73	theme	raw	684:686	arg1	bentonite					688:696	raw bentonite	684:696	raw bentonite (BnR)	684:702	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	5	74	theme	slit-shaped	997:1007	arg1	pores					1009:1013	slit-shaped pores	997:1013	slit-shaped pores	997:1013	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	1	75	theme	toxic	132:136	arg1	metals					144:149	The toxic heavy metals	128:149	The toxic heavy metals contamination in water bodies	128:179	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	0	76	theme	Cu2+	83:86	arg1	removal					66:72	efficient removal	56:72	efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment	56:125	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	1	77	theme	metals	144:149	arg1	contamination					151:163	The toxic heavy metals contamination	128:163	The toxic heavy metals contamination in water bodies	128:179	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	0	78	theme	Pb2+	92:95	arg1	ions					97:100	toxic Cu2+ and Pb2+ ions	77:100	ions	97:100	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	1	79	from	contamination	151:163	arg1	bodies					174:179	water bodies	168:179	water bodies	168:179	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	2	80	from	present	277:283	arg1	environments					296:307	aquatic environments	288:307	aquatic environments due to their extensive usage in various industries for diverse applications	288:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	12	81	theme	Pb2+	2055:2058	arg1	removal					2035:2041	the removal	2031:2041	the removal of Cu2+ and Pb2+ from aqueous waste	2031:2077	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	4	82	theme	activated	705:713	arg1	ABn					726:728	activated bentonite (ABn)	705:729	activated bentonite (ABn)	705:729	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	2	83	theme	aquatic	288:294	arg1	environments					296:307	aquatic environments	288:307	aquatic environments due to their extensive usage in various industries for diverse applications	288:383	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	5	84	theme	IV	902:903	arg1	isotherm					852:859	The nitrogen adsorption-desorption isotherm	817:859	The nitrogen adsorption-desorption isotherm obtained for the materials	817:886	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	5	84	theme	IV	902:903	arg1	isotherm					905:912	the type IV isotherm	893:912	the type IV isotherm with characteristics H3 hysteresis loops	893:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	10	85	from	present	1671:1677	arg1	ABn-AG					1682:1687	ABn-AG	1682:1687	ABn-AG	1682:1687	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	4	86	dep	bentonite	688:696	arg1	i.e.					678:681	i.e.	678:681	i.e.	678:681	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	10	87	from	ABn-AG	1682:1687	arg1	present					1671:1677	present	1671:1677	present	1671:1677	Binary adsorption study suggested that Cu2+ and Pb2+ were removed through different binding sites present in ABn-AG.					
32827615	2	88	theme	various	341:347	arg1	industries					349:358	various industries	341:358	various industries	341:358	Copper and lead are the two common toxic metals present in aquatic environments due to their extensive usage in various industries for diverse applications.					
32827615	5	89	theme	H3	935:936	arg1	loops					949:953	characteristics H3 hysteresis loops	919:953	characteristics H3 hysteresis loops	919:953	The nitrogen adsorption-desorption isotherm obtained for the materials were the type IV isotherm with characteristics H3 hysteresis loops indicating the presence of mesopores with slit-shaped pores.					
32827615	9	90	theme	MgCl2	1471:1475	arg1	presence					1459:1466	The presence	1455:1466	The presence of MgCl2, NaCl and KCl	1455:1489	The presence of MgCl2, NaCl and KCl did not cause significant influence on the removal of Cu2+ and Pb2+ using ABn-AG.					
32827615	7	91	theme	initial	1213:1219	arg1	time					1229:1232	the initial contact time	1209:1232	the initial contact time	1209:1232	The removal was fast during the initial contact time and the adsorption data obtained at various contact time were fit well to the pseudo-second order kinetic model.					
32827615	9	92	theme	NaCl	1478:1481	arg1	presence					1459:1466	The presence	1455:1466	The presence of MgCl2, NaCl and KCl	1455:1489	The presence of MgCl2, NaCl and KCl did not cause significant influence on the removal of Cu2+ and Pb2+ using ABn-AG.					
32827615	3	93	theme	present	390:396	arg1	study					398:402	The present study	386:402	The present study	386:402	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	12	94	from	waste	2073:2077	arg1	removal					2035:2041	the removal	2031:2041	the removal of Cu2+ and Pb2+ from aqueous waste	2031:2077	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	0	95	theme	efficient	56:64	arg1	removal					66:72	efficient removal	56:72	efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment	56:125	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	9	96	theme	KCl	1487:1489	arg1	presence					1459:1466	The presence	1455:1466	The presence of MgCl2, NaCl and KCl	1455:1489	The presence of MgCl2, NaCl and KCl did not cause significant influence on the removal of Cu2+ and Pb2+ using ABn-AG.					
32827615	4	97	theme	XRD	764:766	arg1	analyses					807:814	XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses	764:814	XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses	764:814	Composite beads were prepared by adapting the ionic gelation method and the materials; i.e., raw bentonite (BnR), activated bentonite (ABn) and ABn-AG were characterized by XRD, BET surface area, TGA-DTA, FT-IR, SEM analyses.					
32827615	3	98	theme	ions	458:461	arg1	removal					419:425	the removal	415:425	the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	415:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	1	99	dep	major	195:199	arg1	concerns					201:208	concerns	201:208	concerns	201:208	The toxic heavy metals contamination in water bodies is one of the major concerns in many countries.					
32827615	3	100	theme	heavy	446:450	arg1	ions					458:461	these two toxic heavy metal ions	430:461	these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction	430:588	The present study deals with the removal of these two toxic heavy metal ions using activated bentonite-alginate (ABn-AG) composite beads which are easily separated and recovered after adsorption reaction.					
32827615	12	101	theme	ABn-AG	1962:1967	arg1	adsorbent					2017:2025	adsorbent	2017:2025	adsorbent	2017:2025	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	12	101	theme	ABn-AG	1962:1967	arg1	beads					1979:1983	ABn-AG composite beads	1962:1983	ABn-AG composite beads	1962:1983	Thus, this study suggested that ABn-AG composite beads can be employed as an efficient adsorbent for the removal of Cu2+ and Pb2+ from aqueous waste.					
32827615	9	102	theme	significant	1505:1515	arg1	influence					1517:1525	significant influence	1505:1525	significant influence	1505:1525	The presence of MgCl2, NaCl and KCl did not cause significant influence on the removal of Cu2+ and Pb2+ using ABn-AG.					
32827615	6	103	theme	high	1057:1060	arg1	removal					1070:1076	reasonably high percent removal	1046:1076	reasonably high percent removal	1046:1076	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	6	104	theme	Batch	1016:1020	arg1	experiments					1022:1032	Batch experiments	1016:1032	Batch experiments	1016:1032	Batch experiments showed that reasonably high percent removal was achieved even at highly acidic conditions, i.e., 58% of Cu2+and 77% of Pb2+were removed at pH 2.0.					
32827615	0	105	from	environment	115:125	arg1	removal					66:72	efficient removal	56:72	efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment	56:125	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	0	105	from	environment	115:125	arg1	Cu2+					83:86	toxic Cu2+ and Pb2+ ions	77:100	Cu2+	83:86	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
32827615	0	105	from	environment	115:125	arg1	ions					97:100	toxic Cu2+ and Pb2+ ions	77:100	ions	97:100	Use of activated bentonite-alginate composite beads for efficient removal of toxic Cu2+ and Pb2+ ions from aquatic environment.					
33761584	7	0	theme	autophagy-related	890:906	arg1	protein					908:914	autophagy-related protein	890:914	autophagy-related protein	890:914	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	8	1	theme	fasting	997:1003	arg1	levels					1019:1024	fasting blood glucose levels	997:1024	fasting blood glucose levels	997:1024	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	10	2	theme	muscle	1193:1198	arg1	function					1200:1207	muscle function	1193:1207	muscle function	1193:1207	SR+EX improved glucose tolerance and muscle function and increased serum TC utilization than SR alone.					
33761584	8	3	theme	glucose	1011:1017	arg1	levels					1019:1024	fasting blood glucose levels	997:1024	fasting blood glucose levels	997:1024	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	7	4	theme	chain	860:864	arg1	reaction					866:873	reverse transcription-quantitative polymerase chain reaction	814:873	reverse transcription-quantitative polymerase chain reaction	814:873	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	11	5	theme	proliferator-activated	1337:1358	arg1	receptor					1360:1367	peroxisome proliferator-activated receptor	1326:1367	peroxisome proliferator-activated receptor	1326:1367	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	5	6	theme	body	630:633	arg1	analysis					647:654	body composition analysis	630:654	body composition analysis	630:654	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	6	7	theme	quadriceps	763:772	arg1	muscles					774:780	quadriceps muscles	763:780	quadriceps muscles	763:780	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	7	8	theme	transcription-quantitative	822:847	arg1	reaction					866:873	reverse transcription-quantitative polymerase chain reaction	814:873	reverse transcription-quantitative polymerase chain reaction	814:873	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	1	9	theme	healthy	203:209	arg1	muscles					220:226	healthy skeletal muscles	203:226	healthy skeletal muscles	203:226	BACKGROUND Autophagy maintains muscle mass and healthy skeletal muscles.					
33761584	0	10	theme	High-Fat	116:123	arg1	Mice					150:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	11	11	theme	protein	1386:1392	arg1	expression					1394:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	0	12	theme	Obesity	142:148	arg1	Mice					150:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	12	13	theme	protein	1584:1590	arg1	expression					1592:1601	mitophagy-related protein expression	1566:1601	mitophagy-related protein expression	1566:1601	CONCLUSION SR induced mitophagy-related protein expression in quadriceps, without affecting muscle function.					
33761584	11	14	theme	γ	1369:1369	arg1	expression					1394:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	3	15	with	combination	438:448	arg1	training					469:476	exercise (EX) training	455:476	exercise (EX) training	455:476	We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.					
33761584	9	16	contain	had	1079:1081	arg2	effect					1083:1088	effect	1083:1088	effect	1083:1088	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	9	16	contain	had	1079:1081	arg1	it					1076:1077	it	1076:1077	it	1076:1077	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	2	17	theme	recent	237:242	arg1	studies					244:250	Several recent studies	229:250	Several recent studies	229:250	Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases.					
33761584	5	18	theme	glucose	563:569	arg1	test					581:584	Intraperitoneal glucose tolerance test	547:584	Intraperitoneal glucose tolerance test	547:584	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	0	19	from	Regulation	102:111	arg1	Mice					150:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	12	20	theme	CONCLUSION	1544:1553	arg1	SR					1555:1556	CONCLUSION SR	1544:1556	CONCLUSION SR	1544:1556	CONCLUSION SR induced mitophagy-related protein expression in quadriceps, without affecting muscle function.					
33761584	10	21	theme	serum	1223:1227	arg1	utilization					1232:1242	serum TC utilization	1223:1242	serum TC utilization than SR alone	1223:1256	SR+EX improved glucose tolerance and muscle function and increased serum TC utilization than SR alone.					
33761584	0	22	theme	Beverages	59:67	arg1	Restriction					28:38	Restriction	28:38	Restriction of Sugar-Sweetened Beverages	28:67	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	0	22	theme	Beverages	59:67	arg1	Exercise					15:22	Exercise	15:22	Exercise	15:22	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	2	23	theme	sugar-sweetened	268:282	arg1	consumption					299:309	sugar-sweetened beverage (SSB) consumption	268:309	sugar-sweetened beverage (SSB) consumption	268:309	Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases.					
33761584	1	24	theme	BACKGROUND	156:165	arg1	Autophagy					167:175	BACKGROUND Autophagy	156:175	BACKGROUND Autophagy	156:175	BACKGROUND Autophagy maintains muscle mass and healthy skeletal muscles.					
33761584	3	25	theme	muscle	350:355	arg1	dysfunction					357:367	muscle dysfunction	350:367	muscle dysfunction due to obesity	350:382	We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.					
33761584	0	26	theme	Muscle	72:77	arg1	Function					79:86	Muscle Function	72:86	Muscle Function	72:86	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	5	27	theme	hanging	607:613	arg1	test					620:623	hanging time test	607:623	hanging time test	607:623	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	11	28	theme	liquid	1475:1480	arg1	group					1489:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	11	29	theme	transporter	1303:1313	arg1	type					1315:1318	glucose transporter type 4	1295:1320	glucose transporter type 4	1295:1320	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	6	30	theme	total	694:698	arg1	TC					713:714	TC	713:714	TC	713:714	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	6	30	theme	total	694:698	arg1	cholesterol					700:710	total cholesterol	694:710	total cholesterol (TC)	694:715	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	4	31	theme	Obese	487:491	arg1	mice					493:496	Obese mice	487:496	Obese mice	487:496	METHODS Obese mice were subjected to SR combined with treadmill EX.					
33761584	11	32	theme	high-fat	1449:1456	arg1	group					1489:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	7	33	theme	Western	797:803	arg1	blot					805:808	Western blot	797:808	Western blot	797:808	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	5	34	theme	grip	587:590	arg1	test					601:604	grip strength test	587:604	grip strength test	587:604	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	9	35	theme	serum	1103:1107	arg1	TG					1109:1110	serum TG	1103:1110	serum TG	1103:1110	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	4	36	dep	METHODS	479:485	arg1	subjected					503:511	subjected	503:511	were subjected to SR combined with treadmill EX	498:544	METHODS Obese mice were subjected to SR combined with treadmill EX.					
33761584	6	37	theme	TG	742:743	arg1	concentrations					745:758	TG concentrations	742:758	TG concentrations	742:758	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	6	38	from	concentrations	723:736	arg1	muscles					774:780	quadriceps muscles	763:780	quadriceps muscles	763:780	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	9	39	theme	protein	1134:1140	arg1	expression					1144:1153	BCL2 interacting protein 3 expression	1117:1153	BCL2 interacting protein 3 expression	1117:1153	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	8	40	theme	significant	975:985	arg1	effect					987:992	no significant effect	972:992	no significant effect	972:992	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	10	41	theme	glucose	1171:1177	arg1	tolerance					1179:1187	glucose tolerance	1171:1187	glucose tolerance	1171:1187	SR+EX improved glucose tolerance and muscle function and increased serum TC utilization than SR alone.					
33761584	3	42	dep	training	469:476	arg1	EX					465:466	EX	465:466	EX	465:466	We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.					
33761584	11	43	located	observed	1513:1520	arg1	group					1537:1541	the HFHS+EX group	1525:1541	the HFHS+EX group	1525:1541	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	11	43	located	observed	1513:1520	arg2	this					1500:1503	this	1500:1503	this	1500:1503	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	5	44	theme	composition	635:645	arg1	analysis					647:654	body composition analysis	630:654	body composition analysis	630:654	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	7	45	theme	mRNA	920:923	arg1	expression					925:934	mRNA expression	920:934	mRNA expression	920:934	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	8	46	theme	blood	1005:1009	arg1	levels					1019:1024	fasting blood glucose levels	997:1024	fasting blood glucose levels	997:1024	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	11	47	theme	relative	1433:1440	arg1	strength					1424:1431	grip strength	1419:1431	grip strength relative to the high-fat and high-sucrose liquid (HFHS) group	1419:1493	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	9	48	theme	BCL2	1117:1120	arg1	expression					1144:1153	BCL2 interacting protein 3 expression	1117:1153	BCL2 interacting protein 3 expression	1117:1153	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	11	49	theme	grip	1419:1422	arg1	strength					1424:1431	grip strength	1419:1431	grip strength relative to the high-fat and high-sucrose liquid (HFHS) group	1419:1493	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	11	50	theme	HFHS+EX	1529:1535	arg1	group					1537:1541	the HFHS+EX group	1525:1541	the HFHS+EX group	1525:1541	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	1	51	theme	muscle	187:192	arg1	mass					194:197	muscle mass	187:197	muscle mass	187:197	BACKGROUND Autophagy maintains muscle mass and healthy skeletal muscles.					
33761584	8	52	theme	glucose	1027:1033	arg1	tolerance					1035:1043	glucose tolerance	1027:1043	glucose tolerance	1027:1043	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	11	53	theme	peroxisome	1326:1335	arg1	receptor					1360:1367	peroxisome proliferator-activated receptor	1326:1367	peroxisome proliferator-activated receptor	1326:1367	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	0	54	theme	High-Sucrose-Fed	125:140	arg1	Mice					150:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	7	55	theme	polymerase	849:858	arg1	reaction					866:873	reverse transcription-quantitative polymerase chain reaction	814:873	reverse transcription-quantitative polymerase chain reaction	814:873	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	1	56	theme	skeletal	211:218	arg1	muscles					220:226	healthy skeletal muscles	203:226	healthy skeletal muscles	203:226	BACKGROUND Autophagy maintains muscle mass and healthy skeletal muscles.					
33761584	12	57	theme	muscle	1636:1641	arg1	function					1643:1650	muscle function	1636:1650	muscle function	1636:1650	CONCLUSION SR induced mitophagy-related protein expression in quadriceps, without affecting muscle function.					
33761584	7	58	dep	helped	875:880	arg1	analyze					882:888	analyze	882:888	analyze	882:888	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	0	59	from	Function	79:86	arg1	Mice					150:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	High-Fat High-Sucrose-Fed Obesity Mice	116:153	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	11	60	theme	coactivator-1α	1371:1384	arg1	expression					1394:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	7	61	theme	reverse	814:820	arg1	reaction					866:873	reverse transcription-quantitative polymerase chain reaction	814:873	reverse transcription-quantitative polymerase chain reaction	814:873	Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.					
33761584	11	62	theme	receptor	1360:1367	arg1	expression					1394:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	8	63	contain	had	968:970	arg2	effect					987:992	no significant effect	972:992	no significant effect	972:992	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	8	63	contain	had	968:970	arg1	SR					959:960	RESULTS SR	951:960	RESULTS SR	951:960	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	5	64	theme	Intraperitoneal	547:561	arg1	test					581:584	Intraperitoneal glucose tolerance test	547:584	Intraperitoneal glucose tolerance test	547:584	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	2	65	theme	SSB	294:296	arg1	consumption					299:309	sugar-sweetened beverage (SSB) consumption	268:309	sugar-sweetened beverage (SSB) consumption	268:309	Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases.					
33761584	5	66	theme	tolerance	571:579	arg1	test					581:584	Intraperitoneal glucose tolerance test	547:584	Intraperitoneal glucose tolerance test	547:584	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	10	67	theme	TC	1229:1230	arg1	utilization					1232:1242	serum TC utilization	1223:1242	serum TC utilization than SR alone	1223:1256	SR+EX improved glucose tolerance and muscle function and increased serum TC utilization than SR alone.					
33761584	2	68	theme	beverage	284:291	arg1	consumption					299:309	sugar-sweetened beverage (SSB) consumption	268:309	sugar-sweetened beverage (SSB) consumption	268:309	Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases.					
33761584	12	69	theme	mitophagy-related	1566:1582	arg1	expression					1592:1601	mitophagy-related protein expression	1566:1601	mitophagy-related protein expression	1566:1601	CONCLUSION SR induced mitophagy-related protein expression in quadriceps, without affecting muscle function.					
33761584	4	70	dep	treadmill	533:541	arg1	EX					543:544	treadmill EX	533:544	treadmill EX	533:544	METHODS Obese mice were subjected to SR combined with treadmill EX.					
33761584	8	71	theme	muscle	1050:1055	arg1	function					1057:1064	muscle function	1050:1064	muscle function	1050:1064	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	0	72	from	Effects	4:10	arg1	Function					79:86	Muscle Function	72:86	Muscle Function	72:86	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	0	72	from	Effects	4:10	arg1	Regulation					102:111	Autophagy Regulation	92:111	Autophagy Regulation	92:111	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	13	73	theme	muscle	1737:1742	arg1	function					1744:1751	muscle function	1737:1751	muscle function	1737:1751	And, the combination of SR and EX activated mitophagy-related proteins and improved muscle function.					
33761584	13	74	theme	mitophagy-related	1697:1713	arg1	proteins					1715:1722	mitophagy-related proteins	1697:1722	mitophagy-related proteins	1697:1722	And, the combination of SR and EX activated mitophagy-related proteins and improved muscle function.					
33761584	0	75	theme	Autophagy	92:100	arg1	Regulation					102:111	Autophagy Regulation	92:111	Autophagy Regulation	92:111	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	11	76	theme	P62	1273:1275	arg1	levels					1277:1282	P62 levels	1273:1282	P62 levels	1273:1282	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	6	77	theme	cholesterol	700:710	arg1	concentrations					723:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations	672:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations	672:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	5	78	theme	time	615:618	arg1	test					620:623	hanging time test	607:623	hanging time test	607:623	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	11	79	theme	type	1315:1318	arg1	expression					1394:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression	1295:1403	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	9	80	theme	serum	1093:1097	arg1	TC					1099:1100	serum TC	1093:1100	serum TC	1093:1100	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	3	81	theme	exercise	455:462	arg1	training					469:476	exercise (EX) training	455:476	exercise (EX) training	455:476	We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.					
33761584	11	82	theme	glucose	1295:1301	arg1	type					1315:1318	glucose transporter type 4	1295:1320	glucose transporter type 4	1295:1320	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	11	83	theme	HFHS	1483:1486	arg1	group					1489:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	0	84	theme	Exercise	15:22	arg1	Effects					4:10	The Effects	0:10	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.	0:154	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	5	85	theme	strength	592:599	arg1	test					601:604	grip strength test	587:604	grip strength test	587:604	Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed.					
33761584	3	86	theme	SSB	405:407	arg1	restriction					409:419	SSB restriction	405:419	SSB restriction (SR) alone or in combination with exercise (EX) training	405:476	We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.					
33761584	3	86	theme	SSB	405:407	arg1	SR					422:423	SR	422:423	SR	422:423	We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.					
33761584	6	87	theme	Triglyceride	672:683	arg1	concentrations					723:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations	672:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations	672:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	0	88	theme	Restriction	28:38	arg1	Effects					4:10	The Effects	0:10	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.	0:154	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	11	89	theme	high-sucrose	1462:1473	arg1	group					1489:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	the high-fat and high-sucrose liquid (HFHS) group	1445:1493	SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.					
33761584	13	90	theme	SR	1677:1678	arg1	combination					1662:1672	the combination	1658:1672	the combination of SR	1658:1678	And, the combination of SR and EX activated mitophagy-related proteins and improved muscle function.					
33761584	0	91	theme	Sugar-Sweetened	43:57	arg1	Beverages					59:67	Sugar-Sweetened Beverages	43:67	Sugar-Sweetened Beverages	43:67	The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice.					
33761584	8	92	theme	RESULTS	951:957	arg1	SR					959:960	RESULTS SR	951:960	RESULTS SR	951:960	RESULTS SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function.					
33761584	2	93	theme	Several	229:235	arg1	studies					244:250	Several recent studies	229:250	Several recent studies	229:250	Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases.					
33761584	9	94	theme	interacting	1122:1132	arg1	expression					1144:1153	BCL2 interacting protein 3 expression	1117:1153	BCL2 interacting protein 3 expression	1117:1153	However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression.					
33761584	6	95	from	concentrations	745:758	arg1	muscles					774:780	quadriceps muscles	763:780	quadriceps muscles	763:780	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
33761584	6	96	theme	serum	717:721	arg1	concentrations					723:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations	672:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations	672:736	Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed.					
34077557	8	0	contain	had	1166:1168	arg2	impact					1173:1178	an impact	1170:1178	an impact on the sensory profile and acceptance of the skyr yogurt	1170:1235	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	8	0	contain	had	1166:1168	arg1	type					1148:1151	The type	1144:1151	The type of sweetener	1144:1164	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	6	1	theme	Southeast	1003:1011	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	3	2	theme	mango	619:623	arg1	pulp					625:628	mango pulp	619:628	mango pulp	619:628	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	11	3	theme	studies	1893:1899	arg1	importance					1879:1888	the importance	1875:1888	the importance of studies related to consumer preferences with cultural differences	1875:1957	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	10	4	theme	studies	1749:1755	arg1	lack					1733:1736	a lack	1731:1736	a lack of sensory studies of skyr yogurt	1731:1770	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	7	5	theme	yogurt	1136:1141	arg1	characteristics					1097:1111	the characteristics	1093:1111	the characteristics of the formulated skyr yogurt	1093:1141	In general, the addition of FOS did not impact the characteristics of the formulated skyr yogurt.					
34077557	11	6	theme	cultural	1938:1945	arg1	differences					1947:1957	cultural differences	1938:1957	cultural differences	1938:1957	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	2	7	theme	natural	237:243	arg1	sweeteners					245:254	natural sweeteners	237:254	natural sweeteners	237:254	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	3	8	with	yogurt	607:612	arg1	pulp					625:628	mango pulp	619:628	mango pulp	619:628	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	9	9	theme	Northeastern	1516:1527	arg1	consumers					1529:1537	Northeastern consumers	1516:1537	Northeastern consumers	1516:1537	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	11	10	theme	consumer	1838:1845	arg1	test					1847:1850	the consumer test	1834:1850	the consumer test in this work	1834:1863	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	1	11	theme	different	162:170	arg1	characteristics					180:194	their different sensory characteristics	156:194	their different sensory characteristics	156:194	Skyr yogurts have been gaining prominence because of their different sensory characteristics.					
34077557	6	12	theme	sweetness	887:895	arg1	equivalence					897:907	sweetness equivalence	887:907	sweetness equivalence for each sweetener used	887:931	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	8	13	theme	sweetener	1156:1164	arg1	type					1148:1151	The type	1144:1151	The type of sweetener	1144:1164	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	10	14	theme	skyr	1760:1763	arg1	yogurt					1765:1770	skyr yogurt	1760:1770	skyr yogurt	1760:1770	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	11	15	theme	test	1847:1850	arg1	results					1823:1829	The results	1819:1829	The results of the consumer test in this work	1819:1863	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	7	16	from	impact	1086:1091	arg1	general					1049:1055	general	1049:1055	general	1049:1055	In general, the addition of FOS did not impact the characteristics of the formulated skyr yogurt.					
34077557	4	17	with	formulations	681:692	arg1	pulp					713:716	mango pulp	707:716	mango pulp	707:716	Eight formulations of skyr with mango pulp were developed.					
34077557	2	18	theme	healthy	210:216	arg1	appeal					218:223	their healthy appeal	204:223	their healthy appeal	204:223	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	9	19	theme	mango	1555:1559	arg1	flavor					1561:1566	mango flavor	1555:1566	mango flavor	1555:1566	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	3	20	theme	sucrose	536:542	arg1	addition					495:502	the addition	491:502	the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin	491:565	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	6	21	theme	ideal	853:857	arg1	tests					832:836	The tests	828:836	The tests performed	828:846	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	6	21	theme	ideal	853:857	arg1	sweetness					859:867	ideal sweetness	853:867	ideal sweetness	853:867	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	8	22	theme	bitter	1330:1335	arg1	aftertaste					1337:1346	bitter aftertaste	1330:1346	bitter aftertaste	1330:1346	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	8	23	theme	sensory	1187:1193	arg1	profile					1195:1201	the sensory profile	1183:1201	the sensory profile	1183:1201	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	6	24	theme	Descriptive	947:957	arg1	QDA					969:971	QDA	969:971	QDA	969:971	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	6	24	theme	Descriptive	947:957	arg1	Analysis					959:966	Quantitative Descriptive Analysis	934:966	Quantitative Descriptive Analysis (QDA)	934:972	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	7	25	theme	skyr	1131:1134	arg1	yogurt					1136:1141	the formulated skyr yogurt	1116:1141	the formulated skyr yogurt	1116:1141	In general, the addition of FOS did not impact the characteristics of the formulated skyr yogurt.					
34077557	2	26	theme	prebiotics	441:450	arg1	incorporation					424:436	the incorporation	420:436	the incorporation of prebiotics	420:450	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	8	27	theme	metallic	1353:1360	arg1	flavor					1362:1367	metallic flavor	1353:1367	metallic flavor	1353:1367	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	11	28	from	test	1847:1850	arg1	work					1860:1863	this work	1855:1863	this work	1855:1863	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	9	29	theme	affective	1389:1397	arg1	test					1399:1402	the affective test	1385:1402	the affective test	1385:1402	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	0	30	from	Influence	0:8	arg1	profile					63:69	the sensory profile	51:69	the sensory profile of skyr yogurt with mango pulp	51:100	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	6	31	from	Analysis	959:966	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	4	32	theme	skyr	697:700	arg1	formulations					681:692	Eight formulations	675:692	Eight formulations of skyr with mango pulp	675:716	Eight formulations of skyr with mango pulp were developed.					
34077557	0	33	theme	skyr	74:77	arg1	yogurt					79:84	skyr yogurt	74:84	skyr yogurt	74:84	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	4	34	theme	mango	707:711	arg1	pulp					713:716	mango pulp	707:716	mango pulp	707:716	Eight formulations of skyr with mango pulp were developed.					
34077557	9	35	dep	flavor	1561:1566	arg1	addition					1543:1550	addition	1543:1550	addition	1543:1550	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	0	36	theme	sensory	55:61	arg1	profile					63:69	the sensory profile	51:69	the sensory profile of skyr yogurt with mango pulp	51:100	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	10	37	theme	sensory	1741:1747	arg1	studies					1749:1755	sensory studies	1741:1755	sensory studies of skyr yogurt	1741:1770	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	2	38	theme	sweet	340:344	arg1	taste					346:350	a sweet taste	338:350	a sweet taste	338:350	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	5	39	theme	compositional	738:750	arg1	parameters					752:761	The compositional parameters	734:761	The compositional parameters evaluated	734:771	The compositional parameters evaluated were moisture, protein, lipids, ash, and carbohydrate.					
34077557	5	39	theme	compositional	738:750	arg1	moisture					778:785	moisture	778:785	moisture	778:785	The compositional parameters evaluated were moisture, protein, lipids, ash, and carbohydrate.					
34077557	6	40	from	equivalence	897:907	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	6	41	from	sweetness	859:867	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	3	42	theme	sensory	579:585	arg1	profile					587:593	the sensory profile	575:593	the sensory profile of the skyr yogurt with mango pulp	575:628	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	2	43	dep	increasing	368:377	arg1	addition					356:363	addition	356:363	addition	356:363	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	0	44	theme	sweeteners	37:46	arg1	Influence					0:8	Influence	0:8	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.	0:101	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	6	45	theme	Brazil	1038:1043	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	11	46	from	results	1823:1829	arg1	work					1860:1863	this work	1855:1863	this work	1855:1863	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	2	47	theme	yogurt	291:296	arg1	type					283:286	this type	278:286	this type of yogurt	278:296	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	3	48	theme	skyr	602:605	arg1	yogurt					607:612	the skyr yogurt	598:612	the skyr yogurt with mango pulp	598:628	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	11	49	theme	consumer	1912:1919	arg1	preferences					1921:1931	consumer preferences	1912:1931	consumer preferences with cultural differences	1912:1957	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	0	50	with	profile	63:69	arg1	pulp					97:100	mango pulp	91:100	mango pulp	91:100	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	11	51	theme	related	1901:1907	arg1	studies					1893:1899	studies	1893:1899	studies related to consumer preferences with cultural differences	1893:1957	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	3	52	from	profile	587:593	arg1	regions					666:672	two Brazilian regions	652:672	two Brazilian regions	652:672	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	3	53	theme	Brazilian	656:664	arg1	regions					666:672	two Brazilian regions	652:672	two Brazilian regions	652:672	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	6	54	from	testing	988:994	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	2	55	theme	sweeteners	245:254	arg1	alternative					308:318	an alternative	305:318	an alternative for incorporating a sweet taste	305:350	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	2	55	theme	sweeteners	245:254	arg1	use					230:232	the use	226:232	the use of natural sweeteners to replace sucrose in this type of yogurt	226:296	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	9	56	dep	flavor	1461:1466	arg1	attribute					1482:1490	a positive attribute	1471:1490	a positive attribute in this yogurt	1471:1505	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	3	57	from	acceptance	638:647	arg1	regions					666:672	two Brazilian regions	652:672	two Brazilian regions	652:672	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	10	58	theme	PRACTICAL	1618:1626	arg1	APPLICATION					1628:1638	PRACTICAL APPLICATION	1618:1638	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.	1618:1817	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	10	59	theme	yogurt	1765:1770	arg1	studies					1749:1755	sensory studies	1741:1755	sensory studies of skyr yogurt	1741:1770	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	3	60	theme	fructooligosaccharide	507:527	arg1	addition					495:502	the addition	491:502	the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin	491:565	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	1	61	theme	sensory	172:178	arg1	characteristics					180:194	their different sensory characteristics	156:194	their different sensory characteristics	156:194	Skyr yogurts have been gaining prominence because of their different sensory characteristics.					
34077557	8	62	theme	bitter	1316:1321	arg1	taste					1323:1327	bitter taste	1316:1327	bitter taste	1316:1327	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	10	63	dep	APPLICATION	1628:1638	arg1	useful					1659:1664	useful	1659:1664	useful	1659:1664	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	6	64	theme	mango	873:877	arg1	flavor					879:884	mango flavor	873:884	mango flavor	873:884	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	8	65	theme	sweet	1285:1289	arg1	taste					1291:1295	sweet taste	1285:1295	sweet taste	1285:1295	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	3	66	theme	thaumatin	557:565	arg1	addition					495:502	the addition	491:502	the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin	491:565	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	1	67	theme	Skyr	103:106	arg1	yogurts					108:114	Skyr yogurts	103:114	Skyr yogurts	103:114	Skyr yogurts have been gaining prominence because of their different sensory characteristics.					
34077557	8	68	theme	skyr	1225:1228	arg1	yogurt					1230:1235	the skyr yogurt	1221:1235	the skyr yogurt	1221:1235	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	7	69	theme	formulated	1120:1129	arg1	yogurt					1136:1141	the formulated skyr yogurt	1116:1141	the formulated skyr yogurt	1116:1141	In general, the addition of FOS did not impact the characteristics of the formulated skyr yogurt.					
34077557	9	70	from	consumers	1427:1435	arg1	flavor					1461:1466	the Southeast, mango flavor	1440:1466	the Southeast, mango flavor is a positive attribute in this yogurt	1440:1505	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	6	71	theme	Quantitative	934:945	arg1	QDA					969:971	QDA	969:971	QDA	969:971	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	6	71	theme	Quantitative	934:945	arg1	Analysis					959:966	Quantitative Descriptive Analysis	934:966	Quantitative Descriptive Analysis (QDA)	934:972	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	0	72	theme	yogurt	79:84	arg1	profile					63:69	the sensory profile	51:69	the sensory profile of skyr yogurt with mango pulp	51:100	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	9	73	theme	test	1399:1402	arg1	results					1374:1380	The results	1370:1380	The results of the affective test	1370:1402	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	11	74	from	work	1860:1863	arg1	results					1823:1829	The results	1819:1829	The results of the consumer test in this work	1819:1863	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	0	75	theme	mango	91:95	arg1	pulp					97:100	mango pulp	91:100	mango pulp	91:100	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	8	76	from	impact	1173:1178	arg1	acceptance					1207:1216	acceptance	1207:1216	acceptance	1207:1216	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	8	76	from	impact	1173:1178	arg1	profile					1195:1201	the sensory profile	1183:1201	the sensory profile	1183:1201	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	9	77	from	attribute	1482:1490	arg1	yogurt					1500:1505	this yogurt	1495:1505	this yogurt	1495:1505	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	8	78	theme	yogurt	1230:1235	arg1	acceptance					1207:1216	acceptance	1207:1216	acceptance	1207:1216	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	8	78	theme	yogurt	1230:1235	arg1	profile					1195:1201	the sensory profile	1183:1201	the sensory profile	1183:1201	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	7	79	theme	FOS	1074:1076	arg1	addition					1062:1069	the addition	1058:1069	the addition of FOS	1058:1076	In general, the addition of FOS did not impact the characteristics of the formulated skyr yogurt.					
34077557	6	80	theme	consumer	979:986	arg1	testing					988:994	consumer testing	979:994	consumer testing	979:994	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	0	81	theme	prebiotic	15:23	arg1	sweeteners					37:46	a prebiotic and natural sweeteners	13:46	a prebiotic and natural sweeteners	13:46	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	6	82	from	flavor	879:884	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	0	83	theme	natural	29:35	arg1	sweeteners					37:46	a prebiotic and natural sweeteners	13:46	a prebiotic and natural sweeteners	13:46	Influence of a prebiotic and natural sweeteners on the sensory profile of skyr yogurt with mango pulp.					
34077557	10	84	theme	dairy	1674:1678	arg1	industry					1680:1687	the dairy industry	1670:1687	the dairy industry	1670:1687	PRACTICAL APPLICATION: This study may be useful for the dairy industry because in the literature, there is still a lack of sensory studies of skyr yogurt, especially when sucrose substitutes are used.					
34077557	8	85	theme	mango	1271:1275	arg1	flavor					1277:1282	mango flavor	1271:1282	mango flavor	1271:1282	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
34077557	9	86	theme	mango	1455:1459	arg1	flavor					1461:1466	the Southeast, mango flavor	1440:1466	the Southeast, mango flavor is a positive attribute in this yogurt	1440:1505	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	11	87	with	preferences	1921:1931	arg1	differences					1947:1957	cultural differences	1938:1957	cultural differences	1938:1957	The results of the consumer test in this work reinforce the importance of studies related to consumer preferences with cultural differences.					
34077557	2	88	theme	product	404:410	arg1	functionality					383:395	the functionality	379:395	the functionality of the product	379:410	Due to their healthy appeal, the use of natural sweeteners to replace sucrose in this type of yogurt can be an alternative for incorporating a sweet taste, in addition to increasing the functionality of the product through the incorporation of prebiotics.					
34077557	3	89	theme	yogurt	607:612	arg1	profile					587:593	the sensory profile	575:593	the sensory profile of the skyr yogurt with mango pulp	575:628	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	3	89	theme	yogurt	607:612	arg1	acceptance					638:647	its acceptance	634:647	its acceptance in two Brazilian regions	634:672	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	3	90	theme	stevia	545:550	arg1	addition					495:502	the addition	491:502	the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin	491:565	This study aimed to determine whether the addition of fructooligosaccharide (FOS), sucrose, stevia, and thaumatin affects the sensory profile of the skyr yogurt with mango pulp and its acceptance in two Brazilian regions.					
34077557	9	91	theme	positive	1473:1480	arg1	attribute					1482:1490	a positive attribute	1471:1490	a positive attribute in this yogurt	1471:1505	The results of the affective test demonstrated that, for consumers in the Southeast, mango flavor is a positive attribute in this yogurt, and for Northeastern consumers, in addition to mango flavor, sweetness must also be taken into consideration.					
34077557	6	92	theme	Northeast	1017:1025	arg1	regions					1027:1033	the Southeast and Northeast regions	999:1033	regions	1027:1033	The tests performed were ideal sweetness and mango flavor, sweetness equivalence for each sweetener used, Quantitative Descriptive Analysis (QDA), and consumer testing in the Southeast and Northeast regions of Brazil.					
34077557	8	93	theme	sweet	1298:1302	arg1	aftertaste					1304:1313	sweet aftertaste	1298:1313	sweet aftertaste	1298:1313	The type of sweetener had an impact on the sensory profile and acceptance of the skyr yogurt, affected characteristics such as mango flavor, sweet taste, sweet aftertaste, bitter taste, bitter aftertaste, and metallic flavor.					
35014401	3	0	theme	thermomechanical	760:775	arg1	properties					777:786	thermomechanical properties	760:786	thermomechanical properties	760:786	We also investigate the relationship between structural composition, thermal processing, and thermomechanical properties.					
35014401	8	1	theme	routes	1445:1450	arg1	development					1408:1418	the strategic development	1394:1418	the strategic development of chemical modification routes using commercial polymer processing technologies	1394:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	8	2	theme	stretchable	1670:1680	arg1	applications					1693:1704	stretchable electronic applications	1670:1704	stretchable electronic applications	1670:1704	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	2	3	theme	glycidyl	572:579	arg1	[n-butyl					588:595	[n-butyl	588:595	[n-butyl (BuGE)	588:602	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	3	theme	glycidyl	572:579	arg1	hexyl					636:640	2-ethyl hexyl	628:640	2-ethyl hexyl (EtHGE) glycidyl ether]	628:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	3	theme	glycidyl	572:579	arg1	ethers					581:586	three different glycidyl ethers	556:586	three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	556:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	3	theme	glycidyl	572:579	arg1	isopropyl					605:613	isopropyl	605:613	isopropyl (iPrGE)	605:621	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	8	4	theme	electronic	1682:1691	arg1	applications					1693:1704	stretchable electronic applications	1670:1704	stretchable electronic applications	1670:1704	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	2	5	theme	glycidyl	650:657	arg1	ether					659:663	EtHGE) glycidyl ether	643:663	EtHGE) glycidyl ether	643:663	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	5	theme	glycidyl	650:657	arg1	hexyl					636:640	2-ethyl hexyl	628:640	2-ethyl hexyl (EtHGE) glycidyl ether]	628:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	1	6	theme	processable	198:208	arg1	materials					210:218	flexible, stretchable, and thermally processable materials	161:218	flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers	161:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	1	7	theme	smooth	328:333	arg1	transition					335:344	the smooth transition	324:344	the smooth transition of the fossil-based economy to the circular bioeconomy	324:399	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	0	8	theme	Arabinoxylan-Derived	113:132	arg1	Thermoplastics					134:147	Arabinoxylan-Derived Thermoplastics	113:147	Arabinoxylan-Derived Thermoplastics	113:147	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	8	9	theme	fine-tuning	1508:1518	arg1	structures					1520:1529	fine-tuning structures	1508:1529	fine-tuning structures	1508:1529	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	5	10	theme	%	1026:1026	arg1	levels					1038:1043	19 and 31% oxidation levels	1017:1043	19 and 31% oxidation levels	1017:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	5	11	theme	31	1024:1025	arg1	levels					1038:1043	19 and 31% oxidation levels	1017:1043	19 and 31% oxidation levels	1017:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	8	12	theme	polysaccharide-based	1560:1579	arg1	polymers					1581:1588	specific polysaccharide-based polymers	1551:1588	specific polysaccharide-based polymers	1551:1588	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	2	13	theme	EtHGE	643:647	arg1	ether					659:663	EtHGE) glycidyl ether	643:663	EtHGE) glycidyl ether	643:663	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	13	theme	EtHGE	643:647	arg1	hexyl					636:640	2-ethyl hexyl	628:640	2-ethyl hexyl (EtHGE) glycidyl ether]	628:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	6	14	dep	%	1209:1209	arg1	±19					1212:1214	±19	1212:1214	±19	1212:1214	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	5	15	theme	oxidation	1028:1036	arg1	levels					1038:1043	19 and 31% oxidation levels	1017:1043	19 and 31% oxidation levels	1017:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	4	16	theme	BuGE-	789:793	arg1	dA-AXs					816:821	BuGE- and iPrGE-etherified dA-AXs	789:821	BuGE- and iPrGE-etherified dA-AXs	789:821	BuGE- and iPrGE-etherified dA-AXs showed glass-transition temperatures (Tg) far below their degradation temperatures and gave thermoplastic materials when compression-molded at 140 °C.					
35014401	8	17	theme	specific	1551:1558	arg1	polymers					1581:1588	specific polysaccharide-based polymers	1551:1588	specific polysaccharide-based polymers	1551:1588	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	8	18	theme	processing	1477:1486	arg1	technologies					1488:1499	commercial polymer processing technologies	1458:1499	commercial polymer processing technologies	1458:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	0	19	theme	Thermoplastics	134:147	arg1	Processability					63:76	Thermal Processability and Thermomechanical Properties	55:108	Processability	63:76	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	0	19	theme	Thermoplastics	134:147	arg1	Properties					99:108	Thermal Processability and Thermomechanical Properties	55:108	Properties	99:108	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	6	20	with	samples	1127:1133	arg1	terminals					1161:1169	shorter and branched terminals	1140:1169	shorter and branched terminals in the side chains	1140:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	6	21	theme	shorter	1140:1146	arg1	terminals					1161:1169	shorter and branched terminals	1140:1169	shorter and branched terminals in the side chains	1140:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	2	22	theme	ring-opening	464:475	arg1	oxidation					477:485	ring-opening oxidation	464:485	ring-opening oxidation	464:485	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	0	23	theme	Thermomechanical	82:97	arg1	Properties					99:108	Thermal Processability and Thermomechanical Properties	55:108	Properties	99:108	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	7	24	with	elongation	1268:1277	arg1	Tg					1354:1355	Tg	1354:1355	Tg	1354:1355	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	7	24	with	elongation	1268:1277	arg1	substitution					1337:1348	molar substitution	1331:1348	molar substitution	1331:1348	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	6	25	theme	side	1178:1181	arg1	chains					1183:1188	the side chains	1174:1188	the side chains	1174:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	7	26	theme	superior	1259:1266	arg1	elongation					1268:1277	superior elongation	1259:1277	superior elongation of AX thermoplastic films	1259:1303	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	0	27	theme	Oxidation	0:8	arg1	Level					10:14	Oxidation Level	0:14	Oxidation Level	0:14	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	6	28	theme	60	1207:1208	arg1	%					1209:1209	%	1209:1209	%	1209:1209	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	4	29	theme	iPrGE-etherified	799:814	arg1	dA-AXs					816:821	BuGE- and iPrGE-etherified dA-AXs	789:821	BuGE- and iPrGE-etherified dA-AXs	789:821	BuGE- and iPrGE-etherified dA-AXs showed glass-transition temperatures (Tg) far below their degradation temperatures and gave thermoplastic materials when compression-molded at 140 °C.					
35014401	0	30	theme	Glycidyl	20:27	arg1	Structure					35:43	Glycidyl Ether Structure	20:43	Glycidyl Ether Structure	20:43	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	1	31	theme	fossil-based	353:364	arg1	economy					366:372	the fossil-based economy	349:372	the fossil-based economy	349:372	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	4	32	theme	glass-transition	830:845	arg1	Tg					861:862	Tg	861:862	Tg	861:862	BuGE- and iPrGE-etherified dA-AXs showed glass-transition temperatures (Tg) far below their degradation temperatures and gave thermoplastic materials when compression-molded at 140 °C.					
35014401	4	32	theme	glass-transition	830:845	arg1	temperatures					847:858	glass-transition temperatures	830:858	glass-transition temperatures (Tg) far below their degradation temperatures	830:904	BuGE- and iPrGE-etherified dA-AXs showed glass-transition temperatures (Tg) far below their degradation temperatures and gave thermoplastic materials when compression-molded at 140 °C.					
35014401	6	33	from	terminals	1161:1169	arg1	chains					1183:1188	the side chains	1174:1188	the side chains	1174:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	1	34	theme	stretchable	238:248	arg1	applications					261:272	stretchable electronic applications	238:272	stretchable electronic applications from polysaccharide-based polymers	238:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	5	35	theme	19	1017:1018	arg1	%					1026:1026	%	1026:1026	%	1026:1026	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	1	36	theme	economy	366:372	arg1	transition					335:344	the smooth transition	324:344	the smooth transition of the fossil-based economy to the circular bioeconomy	324:399	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	8	37	theme	commercial	1458:1467	arg1	technologies					1488:1499	commercial polymer processing technologies	1458:1499	commercial polymer processing technologies	1458:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	2	38	theme	AX	427:428	arg1	thermoplastics					437:450	arabinoxylan (AX)-based thermoplastics	413:450	arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	413:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	8	39	theme	bio-based	1611:1619	arg1	products					1621:1628	bio-based products	1611:1628	bio-based products	1611:1628	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	1	40	theme	electronic	250:259	arg1	applications					261:272	stretchable electronic applications	238:272	stretchable electronic applications from polysaccharide-based polymers	238:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	2	41	theme	different	562:570	arg1	[n-butyl					588:595	[n-butyl	588:595	[n-butyl (BuGE)	588:602	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	41	theme	different	562:570	arg1	hexyl					636:640	2-ethyl hexyl	628:640	2-ethyl hexyl (EtHGE) glycidyl ether]	628:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	41	theme	different	562:570	arg1	ethers					581:586	three different glycidyl ethers	556:586	three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	556:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	41	theme	different	562:570	arg1	isopropyl					605:613	isopropyl	605:613	isopropyl (iPrGE)	605:621	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	42	theme	arabinoxylan	413:424	arg1	thermoplastics					437:450	arabinoxylan (AX)-based thermoplastics	413:450	arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	413:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	7	43	theme	AX	1282:1283	arg1	films					1299:1303	AX thermoplastic films	1282:1303	AX thermoplastic films	1282:1303	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	7	44	theme	thermoplastic	1285:1297	arg1	films					1299:1303	AX thermoplastic films	1282:1303	AX thermoplastic films	1282:1303	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	2	45	theme	subsequent	491:500	arg1	dA-AX					513:517	dA-AX	513:517	dA-AX	513:517	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	45	theme	subsequent	491:500	arg1	reduction					502:510	subsequent reduction	491:510	subsequent reduction (dA-AX)	491:518	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	0	46	theme	Ether	29:33	arg1	Structure					35:43	Glycidyl Ether Structure	20:43	Glycidyl Ether Structure	20:43	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	8	47	used	used	1594:1597	arg2	polymers					1581:1588	specific polysaccharide-based polymers	1551:1588	specific polysaccharide-based polymers	1551:1588	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	5	48	theme	-etherified	990:1000	arg1	films					1008:1012	The BuGE (3 mol)-etherified dA-AX films	974:1012	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels	974:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	5	49	dep	%	1053:1053	arg1	elongation					1076:1085	elongation	1076:1085	elongation	1076:1085	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	0	50	theme	Thermal	55:61	arg1	Processability					63:76	Thermal Processability and Thermomechanical Properties	55:108	Processability	63:76	Oxidation Level and Glycidyl Ether Structure Determine Thermal Processability and Thermomechanical Properties of Arabinoxylan-Derived Thermoplastics.					
35014401	1	51	theme	circular	381:388	arg1	bioeconomy					390:399	the circular bioeconomy	377:399	the circular bioeconomy	377:399	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	2	52	dep	ethers	581:586	arg1	ether					659:663	EtHGE) glycidyl ether	643:663	EtHGE) glycidyl ether	643:663	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	52	dep	ethers	581:586	arg1	isopropyl					605:613	isopropyl	605:613	isopropyl (iPrGE)	605:621	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	52	dep	ethers	581:586	arg1	hexyl					636:640	2-ethyl hexyl	628:640	2-ethyl hexyl (EtHGE) glycidyl ether]	628:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	52	dep	ethers	581:586	arg1	ethers					581:586	three different glycidyl ethers	556:586	three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	556:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	52	dep	ethers	581:586	arg1	[n-butyl					588:595	[n-butyl	588:595	[n-butyl (BuGE)	588:602	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	52	dep	ethers	581:586	arg1	iPrGE					616:620	iPrGE	616:620	iPrGE	616:620	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	52	dep	ethers	581:586	arg1	BuGE					598:601	BuGE	598:601	BuGE	598:601	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	8	53	theme	polymer	1469:1475	arg1	technologies					1488:1499	commercial polymer processing technologies	1458:1499	commercial polymer processing technologies	1458:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	4	54	theme	thermoplastic	915:927	arg1	materials					929:937	thermoplastic materials	915:937	thermoplastic materials	915:937	BuGE- and iPrGE-etherified dA-AXs showed glass-transition temperatures (Tg) far below their degradation temperatures and gave thermoplastic materials when compression-molded at 140 °C.					
35014401	6	55	theme	branched	1152:1159	arg1	terminals					1161:1169	shorter and branched terminals	1140:1169	shorter and branched terminals in the side chains	1140:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	7	56	with	relationship	1313:1324	arg1	Tg					1354:1355	Tg	1354:1355	Tg	1354:1355	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	7	56	with	relationship	1313:1324	arg1	substitution					1337:1348	molar substitution	1331:1348	molar substitution	1331:1348	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	8	57	theme	strategic	1398:1406	arg1	development					1408:1418	the strategic development	1394:1418	the strategic development of chemical modification routes using commercial polymer processing technologies	1394:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	1	58	theme	flexible	161:168	arg1	materials					210:218	flexible, stretchable, and thermally processable materials	161:218	flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers	161:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	2	59	theme	2-ethyl	628:634	arg1	ether					659:663	EtHGE) glycidyl ether	643:663	EtHGE) glycidyl ether	643:663	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	59	theme	2-ethyl	628:634	arg1	hexyl					636:640	2-ethyl hexyl	628:640	2-ethyl hexyl (EtHGE) glycidyl ether]	628:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	2	59	theme	2-ethyl	628:634	arg1	ethers					581:586	three different glycidyl ethers	556:586	three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	556:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	3	60	theme	thermal	736:742	arg1	processing					744:753	thermal processing	736:753	thermal processing	736:753	We also investigate the relationship between structural composition, thermal processing, and thermomechanical properties.					
35014401	8	61	theme	chemical	1423:1430	arg1	routes					1445:1450	chemical modification routes	1423:1450	chemical modification routes using commercial polymer processing technologies	1423:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	6	62	theme	elongation	1217:1226	arg1	maximum					1196:1202	a maximum	1194:1202	a maximum of 60% (±19) elongation	1194:1226	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	5	63	theme	dA-AX	1002:1006	arg1	films					1008:1012	The BuGE (3 mol)-etherified dA-AX films	974:1012	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels	974:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	1	64	theme	polysaccharide-based	279:298	arg1	polymers					300:307	polysaccharide-based polymers	279:307	polysaccharide-based polymers	279:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	1	65	from	polymers	300:307	arg1	packaging					224:232	packaging	224:232	packaging	224:232	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	1	65	from	polymers	300:307	arg1	applications					261:272	stretchable electronic applications	238:272	stretchable electronic applications from polysaccharide-based polymers	238:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	3	66	theme	structural	712:721	arg1	composition					723:733	structural composition	712:733	structural composition	712:733	We also investigate the relationship between structural composition, thermal processing, and thermomechanical properties.					
35014401	6	67	theme	%	1209:1209	arg1	elongation					1217:1226	60% (±19) elongation	1207:1226	60% (±19) elongation	1207:1226	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	1	68	theme	stretchable	171:181	arg1	materials					210:218	flexible, stretchable, and thermally processable materials	161:218	flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers	161:307	Developing flexible, stretchable, and thermally processable materials for packaging and stretchable electronic applications from polysaccharide-based polymers contributes to the smooth transition of the fossil-based economy to the circular bioeconomy.					
35014401	6	69	contain	had	1190:1192	arg2	maximum					1196:1202	a maximum	1194:1202	a maximum of 60% (±19) elongation	1194:1226	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	6	69	contain	had	1190:1192	arg1	samples					1127:1133	iPrGE-dA-AX samples	1115:1133	iPrGE-dA-AX samples with shorter and branched terminals in the side chains	1115:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	8	70	contain	have	1373:1376	arg2	implications					1378:1389	implications	1378:1389	implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties	1378:1544	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	8	70	contain	have	1373:1376	arg1	findings					1364:1371	These findings	1358:1371	These findings	1358:1371	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	6	71	theme	iPrGE-dA-AX	1115:1125	arg1	samples					1127:1133	iPrGE-dA-AX samples	1115:1133	iPrGE-dA-AX samples with shorter and branched terminals in the side chains	1115:1188	In contrast, iPrGE-dA-AX samples with shorter and branched terminals in the side chains had a maximum of 60% (±19) elongation.					
35014401	4	72	theme	degradation	881:891	arg1	temperatures					893:904	their degradation temperatures	875:904	their degradation temperatures	875:904	BuGE- and iPrGE-etherified dA-AXs showed glass-transition temperatures (Tg) far below their degradation temperatures and gave thermoplastic materials when compression-molded at 140 °C.					
35014401	7	73	theme	films	1299:1303	arg1	relationship					1313:1324	its relationship	1309:1324	its relationship with molar substitution and Tg	1309:1355	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	7	73	theme	films	1299:1303	arg1	elongation					1268:1277	superior elongation	1259:1277	superior elongation of AX thermoplastic films	1259:1303	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	5	74	theme	BuGE	978:981	arg1	films					1008:1012	The BuGE (3 mol)-etherified dA-AX films	974:1012	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels	974:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
35014401	7	75	theme	molar	1331:1335	arg1	substitution					1337:1348	molar substitution	1331:1348	molar substitution	1331:1348	No studies have reported such superior elongation of AX thermoplastic films and its relationship with molar substitution and Tg.					
35014401	8	76	from	implications	1378:1389	arg1	properties					1535:1544	properties	1535:1544	properties	1535:1544	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	8	76	from	implications	1378:1389	arg1	structures					1520:1529	fine-tuning structures	1508:1529	fine-tuning structures	1508:1529	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	8	76	from	implications	1378:1389	arg1	development					1408:1418	the strategic development	1394:1418	the strategic development of chemical modification routes using commercial polymer processing technologies	1394:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	8	77	theme	modification	1432:1443	arg1	routes					1445:1450	chemical modification routes	1423:1450	chemical modification routes using commercial polymer processing technologies	1423:1499	These findings have implications on the strategic development of chemical modification routes using commercial polymer processing technologies and on fine-tuning structures and properties when specific polysaccharide-based polymers are used to engineer bio-based products for film, packaging, and substrates for stretchable electronic applications.					
35014401	2	78	theme	-based	430:435	arg1	thermoplastics					437:450	arabinoxylan (AX)-based thermoplastics	413:450	arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether]	413:664	We present arabinoxylan (AX)-based thermoplastics obtained by ring-opening oxidation and subsequent reduction (dA-AX) combined with hydrophobization with three different glycidyl ethers [n-butyl (BuGE), isopropyl (iPrGE), and 2-ethyl hexyl (EtHGE) glycidyl ether].					
35014401	5	79	from	levels	1038:1043	arg1	films					1008:1012	The BuGE (3 mol)-etherified dA-AX films	974:1012	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels	974:1043	The BuGE (3 mol)-etherified dA-AX films at 19 and 31% oxidation levels show 244% (±42) and 267% (±72) elongation, respectively.					
34108935	6	0	theme	male	1012:1015	arg1	offspring					1023:1031	The male MatOb offspring	1008:1031	The male MatOb offspring	1008:1031	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	3	1	theme	islet	482:486	arg1	secretion					496:504	offspring islet insulin secretion	472:504	offspring islet insulin secretion	472:504	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	6	2	theme	secretion	1120:1128	arg1	extent					1055:1060	the highest extent	1043:1060	the highest extent of glucose intolerance and lowest glucose-induced insulin secretion	1043:1128	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	9	3	theme	decreased	1500:1508	arg1	secretion					1524:1532	decreased islet insulin secretion	1500:1532	decreased islet insulin secretion	1500:1532	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	7	4	theme	obese	1168:1172	arg1	dams					1174:1177	obese dams	1168:1177	obese dams	1168:1177	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	9	5	from	pattern	1446:1452	arg1	intolerance					1484:1494	glucose intolerance	1476:1494	glucose intolerance	1476:1494	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	9	5	from	pattern	1446:1452	arg1	secretion					1524:1532	decreased islet insulin secretion	1500:1532	decreased islet insulin secretion	1500:1532	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	6	6	theme	glucose-induced	1096:1110	arg1	secretion					1120:1128	lowest glucose-induced insulin secretion	1089:1128	lowest glucose-induced insulin secretion	1089:1128	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	3	7	theme	C57BL/6J	507:514	arg1	dams					523:526	C57BL/6J female dams	507:526	C57BL/6J female dams	507:526	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	4	8	theme	islet	774:778	arg1	insulin-secretion					787:803	islet phasic insulin-secretion	774:803	islet phasic insulin-secretion	774:803	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	10	9	from	effect	1659:1664	arg1	offspring					1635:1643	offspring	1635:1643	offspring	1635:1643	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	9	10	theme	8-weeks-old	1379:1389	arg1	offspring					1391:1399	8-weeks-old offspring	1379:1399	8-weeks-old offspring of obese dams	1379:1413	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	6	11	theme	glucose	1065:1071	arg1	intolerance					1073:1083	glucose intolerance	1065:1083	glucose intolerance	1065:1083	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	8	12	theme	heparan	1283:1289	arg1	glycosaminoglycan					1299:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	2	13	theme	islet	292:296	arg1	secretion					306:314	islet insulin secretion	292:314	islet insulin secretion	292:314	To date, the sex-differences in offspring islet insulin secretion during early life has not been evaluated extensively, particularly prior to weaning at postnatal day 21 (P21).					
34108935	1	14	theme	increased	199:207	arg1	adiposity					209:217	increased adiposity	199:217	increased adiposity	199:217	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	9	15	theme	dams	1410:1413	arg1	offspring					1391:1399	8-weeks-old offspring	1379:1399	8-weeks-old offspring of obese dams	1379:1413	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	5	16	theme	male	842:845	arg1	offspring					862:870	both male and female P21 offspring	837:870	both male and female P21 offspring born to obese dams (MatOb)	837:897	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	16	theme	male	842:845	arg1	MatOb					892:896	MatOb	892:896	MatOb	892:896	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	17	theme	glucose	967:973	arg1	tolerance					975:983	glucose tolerance	967:983	glucose tolerance	967:983	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	18	theme	female	851:856	arg1	offspring					862:870	both male and female P21 offspring	837:870	both male and female P21 offspring born to obese dams (MatOb)	837:897	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	18	theme	female	851:856	arg1	MatOb					892:896	MatOb	892:896	MatOb	892:896	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	19	dep	born	872:875	arg1	offspring					862:870	both male and female P21 offspring	837:870	both male and female P21 offspring born to obese dams (MatOb)	837:897	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	19	dep	born	872:875	arg1	MatOb					892:896	MatOb	892:896	MatOb	892:896	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	0	20	theme	Heparan	62:68	arg1	Glycosaminoglycans					78:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	3	21	theme	prior	571:575	arg1	weeks					565:569	4 weeks	563:569	4 weeks prior to mating	563:585	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	5	22	theme	same-sex	818:825	arg1	controls					827:834	same-sex controls	818:834	same-sex controls	818:834	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	10	23	from	sex-differences	1713:1727	arg1	intolerance					1740:1750	glucose intolerance	1732:1750	glucose intolerance	1732:1750	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	4	24	theme	obese	648:652	arg1	dams					654:657	chow-fed and obese dams	635:657	chow-fed and obese dams	635:657	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	7	25	theme	female	1148:1153	arg1	offspring					1155:1163	P21 female offspring	1144:1163	P21 female offspring of obese dams	1144:1177	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	9	26	theme	sex-differences	1457:1471	arg1	pattern					1446:1452	a similar pattern	1436:1452	a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion	1436:1532	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	0	27	theme	Islet	101:105	arg1	Secretion					115:123	Islet Insulin Secretion	101:123	Islet Insulin Secretion	101:123	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	9	28	theme	glucose	1476:1482	arg1	intolerance					1484:1494	glucose intolerance	1476:1494	glucose intolerance	1476:1494	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	1	29	theme	mothers	145:151	arg1	Offspring					126:134	Offspring	126:134	Offspring of obese mothers	126:151	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	8	30	theme	pancreatic	1272:1281	arg1	glycosaminoglycan					1299:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	8	31	theme	sulfate	1291:1297	arg1	glycosaminoglycan					1299:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	9	32	theme	islet	1510:1514	arg1	secretion					1524:1532	decreased islet insulin secretion	1500:1532	decreased islet insulin secretion	1500:1532	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	0	33	theme	Obese	13:17	arg1	Dams					19:22	Obese Dams	13:22	Obese Dams	13:22	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	8	34	theme	male	1243:1246	arg1	MatOb					1248:1252	male MatOb	1243:1252	male MatOb	1243:1252	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	10	35	theme	pancreatic	1617:1626	arg1	HSG					1628:1630	pancreatic HSG	1617:1630	pancreatic HSG	1617:1630	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	4	36	theme	glucose	739:745	arg1	tolerance					759:767	glucose and insulin tolerance	739:767	glucose and insulin tolerance	739:767	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	2	37	theme	postnatal	403:411	arg1	day					413:415	postnatal day 21	403:418	postnatal day 21 (P21)	403:424	To date, the sex-differences in offspring islet insulin secretion during early life has not been evaluated extensively, particularly prior to weaning at postnatal day 21 (P21).					
34108935	2	37	theme	postnatal	403:411	arg1	P21					421:423	P21	421:423	P21	421:423	To date, the sex-differences in offspring islet insulin secretion during early life has not been evaluated extensively, particularly prior to weaning at postnatal day 21 (P21).					
34108935	4	38	theme	insulin	751:757	arg1	tolerance					759:767	glucose and insulin tolerance	739:767	glucose and insulin tolerance	739:767	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	3	39	theme	obesity	461:467	arg1	role					444:447	the role	440:447	the role of maternal obesity on offspring islet insulin secretion	440:504	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	5	40	theme	sex-specific	939:950	arg1	differences					952:962	sex-specific differences	939:962	sex-specific differences in glucose tolerance and insulin secretion	939:1005	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	10	41	from	changes	1606:1612	arg1	offspring					1635:1643	offspring	1635:1643	offspring	1635:1643	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	6	42	theme	highest	1047:1053	arg1	extent					1055:1060	the highest extent	1043:1060	the highest extent of glucose intolerance and lowest glucose-induced insulin secretion	1043:1128	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	3	43	from	role	444:447	arg1	secretion					496:504	offspring islet insulin secretion	472:504	offspring islet insulin secretion	472:504	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	1	44	theme	decreased	223:231	arg1	function					240:247	decreased β cell function	223:247	decreased β cell function	223:247	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	1	45	theme	higher	160:165	arg1	risks					167:171	higher risks	160:171	higher risks of type 2 diabetes	160:190	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	3	46	theme	insulin	488:494	arg1	secretion					496:504	offspring islet insulin secretion	472:504	offspring islet insulin secretion	472:504	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	1	47	theme	cell	235:238	arg1	function					240:247	decreased β cell function	223:247	decreased β cell function	223:247	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	5	48	theme	body	910:913	arg1	adiposity					915:923	higher body adiposity	903:923	higher body adiposity	903:923	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	6	49	theme	MatOb	1017:1021	arg1	offspring					1023:1031	The male MatOb offspring	1008:1031	The male MatOb offspring	1008:1031	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	7	50	theme	P21	1144:1146	arg1	offspring					1155:1163	P21 female offspring	1144:1163	P21 female offspring of obese dams	1144:1177	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	3	51	theme	offspring	472:480	arg1	secretion					496:504	offspring islet insulin secretion	472:504	offspring islet insulin secretion	472:504	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	4	52	theme	phasic	780:785	arg1	insulin-secretion					787:803	islet phasic insulin-secretion	774:803	islet phasic insulin-secretion	774:803	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	7	53	theme	dams	1174:1177	arg1	offspring					1155:1163	P21 female offspring	1144:1163	P21 female offspring of obese dams	1144:1177	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	5	54	contain	had	899:901	arg1	MatOb					892:896	MatOb	892:896	MatOb	892:896	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	54	contain	had	899:901	arg1	offspring					862:870	both male and female P21 offspring	837:870	both male and female P21 offspring born to obese dams (MatOb)	837:897	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	54	contain	had	899:901	arg2	adiposity					915:923	higher body adiposity	903:923	higher body adiposity	903:923	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	3	55	theme	female	516:521	arg1	dams					523:526	C57BL/6J female dams	507:526	C57BL/6J female dams	507:526	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	6	56	theme	insulin	1112:1118	arg1	secretion					1120:1128	lowest glucose-induced insulin secretion	1089:1128	lowest glucose-induced insulin secretion	1089:1128	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	10	57	theme	lasting	1651:1657	arg1	effect					1659:1664	a lasting effect	1649:1664	a lasting effect on offspring insulin secretion	1649:1695	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	1	58	theme	type	176:179	arg1	diabetes					183:190	type 2 diabetes	176:190	type 2 diabetes	176:190	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	6	59	theme	lowest	1089:1094	arg1	secretion					1120:1128	lowest glucose-induced insulin secretion	1089:1128	lowest glucose-induced insulin secretion	1089:1128	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	10	60	theme	glucose	1732:1738	arg1	intolerance					1740:1750	glucose intolerance	1732:1750	glucose intolerance	1732:1750	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	1	61	theme	diabetes	183:190	arg1	risks					167:171	higher risks	160:171	higher risks of type 2 diabetes	160:190	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	6	62	theme	intolerance	1073:1083	arg1	extent					1055:1060	the highest extent	1043:1060	the highest extent of glucose intolerance and lowest glucose-induced insulin secretion	1043:1128	The male MatOb offspring developed the highest extent of glucose intolerance and lowest glucose-induced insulin secretion.					
34108935	2	63	theme	insulin	298:304	arg1	secretion					306:314	islet insulin secretion	292:314	islet insulin secretion	292:314	To date, the sex-differences in offspring islet insulin secretion during early life has not been evaluated extensively, particularly prior to weaning at postnatal day 21 (P21).					
34108935	8	64	contain	had	1254:1256	arg2	macromolecule					1329:1341	a macromolecule	1327:1341	a macromolecule critical for islet health	1327:1367	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	8	64	contain	had	1254:1256	arg1	MatOb					1248:1252	male MatOb	1243:1252	male MatOb	1243:1252	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	8	64	contain	had	1254:1256	arg2	decrease					1260:1267	a decrease	1258:1267	a decrease	1258:1267	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	10	65	theme	maternal	1568:1575	arg1	obesity					1577:1583	maternal obesity	1568:1583	maternal obesity	1568:1583	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	5	66	theme	insulin	989:995	arg1	secretion					997:1005	insulin secretion	989:1005	insulin secretion	989:1005	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	9	67	theme	obese	1404:1408	arg1	dams					1410:1413	obese dams	1404:1413	obese dams	1404:1413	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	3	68	theme	western	545:551	arg1	diet					553:556	western diet	545:556	western diet	545:556	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	4	69	theme	dams	654:657	arg1	offspring					622:630	offspring	622:630	offspring of chow-fed and obese dams	622:657	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	7	70	theme	unimpaired	1183:1192	arg1	secretion					1202:1210	unimpaired insulin secretion	1183:1210	unimpaired insulin secretion	1183:1210	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	1	71	theme	obese	139:143	arg1	mothers					145:151	obese mothers	139:151	obese mothers	139:151	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	0	72	theme	Sulfate	70:76	arg1	Glycosaminoglycans					78:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	9	73	theme	similar	1438:1444	arg1	pattern					1446:1452	a similar pattern	1436:1452	a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion	1436:1532	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	5	74	from	differences	952:962	arg1	tolerance					975:983	glucose tolerance	967:983	glucose tolerance	967:983	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	74	from	differences	952:962	arg1	secretion					997:1005	insulin secretion	989:1005	insulin secretion	989:1005	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	0	75	theme	Insulin	107:113	arg1	Secretion					115:123	Islet Insulin Secretion	101:123	Islet Insulin Secretion	101:123	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	7	76	contain	had	1179:1181	arg2	secretion					1202:1210	unimpaired insulin secretion	1183:1210	unimpaired insulin secretion	1183:1210	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	7	76	contain	had	1179:1181	arg1	offspring					1155:1163	P21 female offspring	1144:1163	P21 female offspring of obese dams	1144:1177	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	5	77	theme	obese	880:884	arg1	dams					886:889	obese dams	880:889	obese dams	880:889	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	3	78	theme	maternal	452:459	arg1	obesity					461:467	maternal obesity	452:467	maternal obesity	452:467	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	0	79	from	Sex-Differences	32:46	arg1	Glycosaminoglycans					78:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	0	79	from	Sex-Differences	32:46	arg1	Secretion					115:123	Islet Insulin Secretion	101:123	Islet Insulin Secretion	101:123	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	4	80	theme	prior	700:704	arg1	day					687:689	postnatal day 21	677:692	postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion	677:803	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	4	80	theme	prior	700:704	arg1	P21					695:697	P21	695:697	P21	695:697	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	10	81	theme	sex-specific	1593:1604	arg1	changes					1606:1612	sex-specific changes	1593:1612	sex-specific changes to pancreatic HSG in offspring	1593:1643	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	5	82	theme	P21	858:860	arg1	offspring					862:870	both male and female P21 offspring	837:870	both male and female P21 offspring born to obese dams (MatOb)	837:897	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	5	82	theme	P21	858:860	arg1	MatOb					892:896	MatOb	892:896	MatOb	892:896	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	4	83	theme	postnatal	677:685	arg1	day					687:689	postnatal day 21	677:692	postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion	677:803	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	4	83	theme	postnatal	677:685	arg1	P21					695:697	P21	695:697	P21	695:697	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	0	84	theme	Dams	19:22	arg1	Offspring					0:8	Offspring	0:8	Offspring of Obese Dams	0:22	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	9	85	theme	insulin	1516:1522	arg1	secretion					1524:1532	decreased islet insulin secretion	1500:1532	decreased islet insulin secretion	1500:1532	Notably, 8-weeks-old offspring of obese dams continued to exhibit a similar pattern of sex-differences in glucose intolerance and decreased islet insulin secretion.					
34108935	3	86	theme	maternal	597:604	arg1	obesity					606:612	maternal obesity	597:612	maternal obesity	597:612	To determine the role of maternal obesity on offspring islet insulin secretion, C57BL/6J female dams were fed chow or western diet from 4 weeks prior to mating to induce maternal obesity.					
34108935	10	87	theme	insulin	1679:1685	arg1	secretion					1687:1695	insulin secretion	1679:1695	insulin secretion	1679:1695	Overall, our study suggests that maternal obesity induces sex-specific changes to pancreatic HSG in offspring and a lasting effect on offspring insulin secretion, leading to the sex-differences in glucose intolerance.					
34108935	4	88	theme	chow-fed	635:642	arg1	dams					654:657	chow-fed and obese dams	635:657	chow-fed and obese dams	635:657	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	0	89	theme	Pancreatic	51:60	arg1	Glycosaminoglycans					78:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Pancreatic Heparan Sulfate Glycosaminoglycans	51:95	Offspring of Obese Dams Exhibit Sex-Differences in Pancreatic Heparan Sulfate Glycosaminoglycans and Islet Insulin Secretion.					
34108935	4	90	theme	body	721:724	arg1	composition					726:736	body composition	721:736	body composition	721:736	First, offspring of chow-fed and obese dams were evaluated on postnatal day 21 (P21) prior to weaning for body composition, glucose and insulin tolerance, and islet phasic insulin-secretion.					
34108935	7	91	theme	insulin	1194:1200	arg1	secretion					1202:1210	unimpaired insulin secretion	1183:1210	unimpaired insulin secretion	1183:1210	In contrast, P21 female offspring of obese dams had unimpaired insulin secretion.					
34108935	8	92	theme	critical	1343:1350	arg1	macromolecule					1329:1341	a macromolecule	1327:1341	a macromolecule critical for islet health	1327:1367	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	8	92	theme	critical	1343:1350	arg1	decrease					1260:1267	a decrease	1258:1267	a decrease	1258:1267	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	2	93	theme	early	323:327	arg1	life					329:332	early life	323:332	early life	323:332	To date, the sex-differences in offspring islet insulin secretion during early life has not been evaluated extensively, particularly prior to weaning at postnatal day 21 (P21).					
34108935	8	94	from	decrease	1260:1267	arg1	glycosaminoglycan					1299:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	pancreatic heparan sulfate glycosaminoglycan	1272:1315	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
34108935	1	95	theme	β	233:233	arg1	function					240:247	decreased β cell function	223:247	decreased β cell function	223:247	Offspring of obese mothers suffer higher risks of type 2 diabetes due to increased adiposity and decreased β cell function.					
34108935	5	96	theme	higher	903:908	arg1	adiposity					915:923	higher body adiposity	903:923	higher body adiposity	903:923	Compared to same-sex controls, both male and female P21 offspring born to obese dams (MatOb) had higher body adiposity and exhibited sex-specific differences in glucose tolerance and insulin secretion.					
34108935	8	97	theme	islet	1356:1360	arg1	health					1362:1367	islet health	1356:1367	islet health	1356:1367	Using SAX-HPLC, we found that male MatOb had a decrease in pancreatic heparan sulfate glycosaminoglycan, which is a macromolecule critical for islet health.					
32331660	6	0	theme	metabolites	839:849	arg1	analysis					814:821	0, 24, 48 and 72 h. Principal component (PC) analysis	769:821	0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites	769:849	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	3	1	theme	colon	513:517	arg1	model					494:498	a dynamic, validated in vitro model	464:498	a dynamic, validated in vitro model of the human colon (TIM-2)	464:525	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	3	2	from	changes	384:390	arg1	microbiota					399:408	gut microbiota	395:408	gut microbiota	395:408	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	2	3	theme	Agave	271:275	arg1	DF					340:341	soluble DF	332:341	soluble DF	332:341	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	2	3	theme	Agave	271:275	arg1	fructans					277:284	Agave fructans	271:284	Agave fructans (AF)	271:289	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	2	3	theme	Agave	271:275	arg1	oligofructans					295:307	oligofructans	295:307	oligofructans (OF)	295:312	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	2	3	theme	Agave	271:275	arg1	AF					287:288	AF	287:288	AF	287:288	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	10	4	theme	relative	1449:1456	arg1	abundance					1458:1466	a high relative abundance	1442:1466	a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate	1442:1584	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	13	5	theme	reliable	1974:1981	arg1	view					1983:1986	a complete and reliable view	1959:1986	a complete and reliable view of the potential prebiotic effect of different dietary fibers	1959:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	13	6	from	Evaluation	1820:1829	arg1	metabolites					1849:1859	metabolites	1849:1859	metabolites	1849:1859	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	13	6	from	Evaluation	1820:1829	arg1	composition					1880:1890	gut microbiota composition	1865:1890	gut microbiota composition	1865:1890	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	8	7	from	ratio	1124:1128	arg1	Hb					1142:1143	Hb	1142:1143	Hb	1142:1143	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	5	8	theme	acids	662:666	arg1	Production					630:639	Production	630:639	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia	630:723	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	3	9	theme	regions	558:564	arg1	sequencing					534:543	sequencing	534:543	sequencing of the V3-V4 regions of the 16S rRNA gene	534:585	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	0	10	theme	human	166:170	arg1	colon					172:176	the human colon	162:176	the human colon	162:176	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	13	11	theme	prebiotic	2005:2013	arg1	effect					2015:2020	the potential prebiotic effect	1991:2020	the potential prebiotic effect of different dietary fibers	1991:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	3	12	theme	rRNA	577:580	arg1	gene					582:585	the 16S rRNA gene	569:585	the 16S rRNA gene	569:585	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	3	13	dep	dynamic	466:472	arg1	validated					475:483	validated	475:483	validated	475:483	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	10	14	theme	bacteria	1318:1325	arg1	genera					1327:1332	About 45 bacteria genera	1309:1332	About 45 bacteria genera	1309:1332	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	3	15	theme	Mix	449:451	arg1	AF					439:440	AF	439:440	AF	439:440	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	3	15	theme	Mix	449:451	arg1	Hb					435:436	predigested Hb	423:436	predigested Hb	423:436	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	0	16	dep	in	136:137	arg1	vitro					139:143	vitro	139:143	vitro	139:143	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	0	17	theme	in	136:137	arg1	TIM-2					152:156	TIM-2	152:156	TIM-2	152:156	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	0	17	theme	in	136:137	arg1	model					145:149	a dynamic in vitro model	126:149	a dynamic in vitro model (TIM-2) of the human colon	126:176	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	3	18	theme	dynamic	466:472	arg1	model					494:498	a dynamic, validated in vitro model	464:498	a dynamic, validated in vitro model of the human colon (TIM-2)	464:525	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	10	19	from	abundance	1458:1466	arg1	Catenibacterium					1504:1518	Catenibacterium	1504:1518	Catenibacterium	1504:1518	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	10	19	from	abundance	1458:1466	arg1	Bacteroides					1488:1498	Bacteroides	1488:1498	Bacteroides	1488:1498	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	8	20	theme	butyric	1066:1072	arg1	acid					1074:1077	butyric acid	1066:1077	The highest butyric acid production	1054:1088	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	0	21	theme	L	61:61	arg1	calyces					63:69	predigested Hibiscus sabdariffa L calyces	29:69	predigested Hibiscus sabdariffa L calyces	29:69	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	5	22	theme	fatty	692:696	arg1	BSCFAs					705:710	BSCFAs	705:710	BSCFAs	705:710	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	5	22	theme	fatty	692:696	arg1	acids					698:702	branched-chain fatty acids	677:702	branched-chain fatty acids (BSCFAs)	677:711	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	10	23	theme	substrate	1576:1584	arg1	time					1558:1561	the fermentation time	1541:1561	the fermentation time depending of substrate	1541:1584	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	0	24	from	Changes	0:6	arg1	microbiota					15:24	gut microbiota	11:24	gut microbiota	11:24	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	0	24	from	Changes	0:6	arg1	fructans					105:112	Agave (Agave tequilana weber) fructans	75:112	Agave (Agave tequilana weber) fructans	75:112	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	0	24	from	Changes	0:6	arg1	calyces					63:69	predigested Hibiscus sabdariffa L calyces	29:69	predigested Hibiscus sabdariffa L calyces	29:69	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	9	25	theme	studied	1255:1261	arg1	substrates					1263:1272	the studied substrates	1251:1272	the studied substrates	1251:1272	BSCFAS were also produced upon feeding the studied substrates, but in much lower concentrations.					
32331660	10	26	theme	these	1360:1364	arg1	changing					1389:1396	changing	1389:1396	changing	1389:1396	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	10	26	theme	these	1360:1364	arg1	these					1360:1364	these	1360:1364	these	1360:1364	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	10	26	theme	these	1360:1364	arg1	10					1354:1355	10	1354:1355	10	1354:1355	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	0	27	theme	tequilana	88:96	arg1	weber					98:102	Agave tequilana weber	82:102	Agave tequilana weber	82:102	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	13	28	theme	validated	1925:1933	arg1	model					1944:1948	a validated in vitro model	1923:1948	a validated in vitro model	1923:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	5	29	theme	ammonia	717:723	arg1	Production					630:639	Production	630:639	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia	630:723	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	6	30	theme	PC	810:811	arg1	analysis					814:821	0, 24, 48 and 72 h. Principal component (PC) analysis	769:821	0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites	769:849	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	2	31	theme	soluble	332:338	arg1	DF					340:341	soluble DF	332:341	soluble DF	332:341	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	2	31	theme	soluble	332:338	arg1	fructans					277:284	Agave fructans	271:284	Agave fructans (AF)	271:289	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	2	31	theme	soluble	332:338	arg1	oligofructans					295:307	oligofructans	295:307	oligofructans (OF)	295:312	Agave fructans (AF) and oligofructans (OF) are considered as soluble DF.					
32331660	13	32	from	metabolites	1849:1859	arg1	Evaluation					1820:1829	Evaluation	1820:1829	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model	1820:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	0	33	theme	gut	11:13	arg1	microbiota					15:24	gut microbiota	11:24	gut microbiota	11:24	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	9	34	theme	lower	1287:1291	arg1	concentrations					1293:1306	much lower concentrations	1282:1306	much lower concentrations	1282:1306	BSCFAS were also produced upon feeding the studied substrates, but in much lower concentrations.					
32331660	6	35	theme	component	799:807	arg1	analysis					814:821	0, 24, 48 and 72 h. Principal component (PC) analysis	769:821	0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites	769:849	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	13	36	from	changes	1838:1844	arg1	metabolites					1849:1859	metabolites	1849:1859	metabolites	1849:1859	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	13	36	from	changes	1838:1844	arg1	composition					1880:1890	gut microbiota composition	1865:1890	gut microbiota composition	1865:1890	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	6	37	theme	Principal	789:797	arg1	analysis					814:821	0, 24, 48 and 72 h. Principal component (PC) analysis	769:821	0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites	769:849	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	13	38	theme	different	2025:2033	arg1	fibers					2043:2048	different dietary fibers	2025:2048	different dietary fibers	2025:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	1	39	theme	fiber	236:240	arg1	compounds					260:268	phenolic compounds	251:268	phenolic compounds	251:268	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	1	39	theme	fiber	236:240	arg1	calyces					204:210	Hibiscus sabdariffa (Hb) calyces	179:210	Hibiscus sabdariffa (Hb) calyces	179:210	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	1	39	theme	fiber	236:240	arg1	source					218:223	a source	216:223	a source of dietary fiber (DF)	216:245	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	13	40	theme	colonic	1899:1905	arg1	fermentation					1907:1918	colonic fermentation	1899:1918	colonic fermentation in a validated in vitro model	1899:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	13	41	from	composition	1880:1890	arg1	Evaluation					1820:1829	Evaluation	1820:1829	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model	1820:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	8	42	theme	SCFAS	1133:1137	arg1	ratio					1124:1128	the molar ratio	1114:1128	the molar ratio of SCFAS on Hb	1114:1143	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	8	42	theme	SCFAS	1133:1137	arg1	63:18:18					1149:1156	63:18:18	1149:1156	63:18:18	1149:1156	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	10	43	theme	fermentation	1409:1420	arg1	time					1422:1425	the fermentation time	1405:1425	the fermentation time	1405:1425	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	0	44	theme	Hibiscus	41:48	arg1	calyces					63:69	predigested Hibiscus sabdariffa L calyces	29:69	predigested Hibiscus sabdariffa L calyces	29:69	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	6	45	theme	genera	877:882	arg1	abundance					864:872	relative abundance	855:872	relative abundance of genera	855:882	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	3	46	theme	human	507:511	arg1	TIM-2					520:524	TIM-2	520:524	TIM-2	520:524	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	3	46	theme	human	507:511	arg1	colon					513:517	the human colon	503:517	the human colon (TIM-2)	503:525	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	4	47	theme	fecal	603:607	arg1	microbiota					609:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota was used.					
32331660	3	48	theme	study	359:363	arg1	aim					348:350	The aim	344:350	The aim of the study	344:363	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	13	49	theme	gut	1865:1867	arg1	composition					1880:1890	gut microbiota composition	1865:1890	gut microbiota composition	1865:1890	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	4	50	theme	human	597:601	arg1	microbiota					609:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota was used.					
32331660	3	51	theme	gut	395:397	arg1	microbiota					399:408	gut microbiota	395:408	gut microbiota	395:408	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	13	52	theme	dietary	2035:2041	arg1	fibers					2043:2048	different dietary fibers	2025:2048	different dietary fibers	2025:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	3	53	dep	in	485:486	arg1	vitro					488:492	vitro	488:492	vitro	488:492	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	7	54	theme	different	1022:1030	arg1	concentrations					1038:1051	different SCFAS concentrations	1022:1051	different SCFAS concentrations	1022:1051	Fermentation of samples resulted in different SCFAS concentrations.					
32331660	13	55	theme	changes	1838:1844	arg1	Evaluation					1820:1829	Evaluation	1820:1829	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model	1820:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	6	56	theme	fermentation	826:837	arg1	metabolites					839:849	fermentation metabolites	826:849	fermentation metabolites	826:849	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	3	57	theme	predigested	423:433	arg1	AF					439:440	AF	439:440	AF	439:440	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	3	57	theme	predigested	423:433	arg1	Hb					435:436	predigested Hb	423:436	predigested Hb	423:436	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	13	58	theme	complete	1961:1968	arg1	view					1983:1986	a complete and reliable view	1959:1986	a complete and reliable view of the potential prebiotic effect of different dietary fibers	1959:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	10	59	theme	high	1444:1447	arg1	abundance					1458:1466	a high relative abundance	1442:1466	a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate	1442:1584	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	4	60	used	used	624:627	arg2	microbiota					609:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota was used.					
32331660	5	61	theme	fatty	656:660	arg1	acids					662:666	short-chain fatty acids	644:666	short-chain fatty acids (SCFAs)	644:674	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	5	61	theme	fatty	656:660	arg1	SCFAs					669:673	SCFAs	669:673	SCFAs	669:673	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	12	62	theme	fermentation	1702:1713	arg1	24 h					1694:1697	24 h	1694:1697	24 h of fermentation	1694:1713	Two PCs were identified: after 24 h of fermentation PC1 was highly influenced by Bifidobacterium and Prevotella, which was related with Hb and SIEM feeding.					
32331660	6	63	theme	extracted	905:913	arg1	factors					915:921	extracted factors	905:921	extracted factors	905:921	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	0	64	theme	colon	172:176	arg1	TIM-2					152:156	TIM-2	152:156	TIM-2	152:156	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	0	64	theme	colon	172:176	arg1	model					145:149	a dynamic in vitro model	126:149	a dynamic in vitro model (TIM-2) of the human colon	126:176	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	13	65	theme	fibers	2043:2048	arg1	effect					2015:2020	the potential prebiotic effect	1991:2020	the potential prebiotic effect of different dietary fibers	1991:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	6	66	theme	variance	976:983	arg1	variance					976:983	variance	976:983	variance	976:983	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	6	66	theme	variance	976:983	arg1	%					971:971	>60%	968:971	>60% of variance	968:983	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	13	67	theme	potential	1995:2003	arg1	effect					2015:2020	the potential prebiotic effect	1991:2020	the potential prebiotic effect of different dietary fibers	1991:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	8	68	theme	acetic	1162:1167	arg1	acid					1192:1195	acetic, propionic and butyric acid	1162:1195	acetic, propionic and butyric acid	1162:1195	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	3	69	theme	V3-V4	552:556	arg1	regions					558:564	the V3-V4 regions	548:564	the V3-V4 regions of the 16S rRNA gene	548:585	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	13	70	theme	effect	2015:2020	arg1	view					1983:1986	a complete and reliable view	1959:1986	a complete and reliable view of the potential prebiotic effect of different dietary fibers	1959:2048	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	8	71	theme	propionic	1170:1178	arg1	acid					1192:1195	acetic, propionic and butyric acid	1162:1195	acetic, propionic and butyric acid	1162:1195	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	12	72	theme	SIEM	1806:1809	arg1	feeding					1811:1817	SIEM feeding	1806:1817	SIEM feeding	1806:1817	Two PCs were identified: after 24 h of fermentation PC1 was highly influenced by Bifidobacterium and Prevotella, which was related with Hb and SIEM feeding.					
32331660	3	73	theme	gene	582:585	arg1	regions					558:564	the V3-V4 regions	548:564	the V3-V4 regions of the 16S rRNA gene	548:585	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	0	74	theme	dynamic	128:134	arg1	TIM-2					152:156	TIM-2	152:156	TIM-2	152:156	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	0	74	theme	dynamic	128:134	arg1	model					145:149	a dynamic in vitro model	126:149	a dynamic in vitro model (TIM-2) of the human colon	126:176	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	5	75	theme	acids	698:702	arg1	Production					630:639	Production	630:639	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia	630:723	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	13	76	dep	in	1935:1936	arg1	vitro					1938:1942	vitro	1938:1942	vitro	1938:1942	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	8	77	theme	butyric	1184:1190	arg1	acid					1192:1195	acetic, propionic and butyric acid	1162:1195	acetic, propionic and butyric acid	1162:1195	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	3	78	theme	16S	573:575	arg1	gene					582:585	the 16S rRNA gene	569:585	the 16S rRNA gene	569:585	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	10	79	theme	fermentation	1545:1556	arg1	time					1558:1561	the fermentation time	1541:1561	the fermentation time depending of substrate	1541:1584	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	8	80	theme	highest	1058:1064	arg1	production					1079:1088	The highest butyric acid production	1054:1088	The highest butyric acid production	1054:1088	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	0	81	theme	Agave	75:79	arg1	fructans					105:112	Agave (Agave tequilana weber) fructans	75:112	Agave (Agave tequilana weber) fructans	75:112	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	1	82	theme	phenolic	251:258	arg1	compounds					260:268	phenolic compounds	251:268	phenolic compounds	251:268	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	5	83	theme	branched-chain	677:690	arg1	BSCFAs					705:710	BSCFAs	705:710	BSCFAs	705:710	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	5	83	theme	branched-chain	677:690	arg1	acids					698:702	branched-chain fatty acids	677:702	branched-chain fatty acids (BSCFAs)	677:711	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	8	84	theme	acid	1074:1077	arg1	production					1079:1088	The highest butyric acid production	1054:1088	The highest butyric acid production	1054:1088	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	0	85	theme	Agave	82:86	arg1	weber					98:102	Agave tequilana weber	82:102	Agave tequilana weber	82:102	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	13	86	from	fermentation	1907:1918	arg1	model					1944:1948	a validated in vitro model	1923:1948	a validated in vitro model	1923:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	1	87	theme	Hibiscus	179:186	arg1	sabdariffa					188:197	Hibiscus sabdariffa	179:197	Hibiscus sabdariffa (Hb) calyces	179:210	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	1	87	theme	Hibiscus	179:186	arg1	Hb					200:201	Hb	200:201	Hb	200:201	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	1	88	theme	sabdariffa	188:197	arg1	calyces					204:210	Hibiscus sabdariffa (Hb) calyces	179:210	Hibiscus sabdariffa (Hb) calyces	179:210	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	1	88	theme	sabdariffa	188:197	arg1	source					218:223	a source	216:223	a source of dietary fiber (DF)	216:245	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	0	89	dep	Agave	75:79	arg1	weber					98:102	Agave tequilana weber	82:102	Agave tequilana weber	82:102	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	10	90	dep	changing	1389:1396	arg1	abundant					1380:1387	abundant	1380:1387	abundant	1380:1387	About 45 bacteria genera were identified and 10 of these were the most abundant changing during the fermentation time, amongst which a high relative abundance in Bifidobacterium, Bacteroides and Catenibacterium, that changed during the fermentation time depending of substrate.					
32331660	13	91	theme	in	1935:1936	arg1	model					1944:1948	a validated in vitro model	1923:1948	a validated in vitro model	1923:1948	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	3	92	theme	in	485:486	arg1	model					494:498	a dynamic, validated in vitro model	464:498	a dynamic, validated in vitro model of the human colon (TIM-2)	464:525	The aim of the study was to investigate changes in gut microbiota upon feeding predigested Hb, AF, OF or Mix (Hb/AF) to a dynamic, validated in vitro model of the human colon (TIM-2), using sequencing of the V3-V4 regions of the 16S rRNA gene.					
32331660	11	93	theme	abundant	1647:1654	arg1	genus					1656:1660	the most abundant genus	1638:1660	the most abundant genus	1638:1660	Hb feeding after 48 h led to Bifidobacterium being the most abundant genus.					
32331660	0	94	theme	predigested	29:39	arg1	calyces					63:69	predigested Hibiscus sabdariffa L calyces	29:69	predigested Hibiscus sabdariffa L calyces	29:69	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	1	95	theme	dietary	228:234	arg1	DF					243:244	DF	243:244	DF	243:244	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	1	95	theme	dietary	228:234	arg1	fiber					236:240	dietary fiber	228:240	dietary fiber (DF)	228:245	Hibiscus sabdariffa (Hb) calyces are a source of dietary fiber (DF) and phenolic compounds.					
32331660	7	96	theme	SCFAS	1032:1036	arg1	concentrations					1038:1051	different SCFAS concentrations	1022:1051	different SCFAS concentrations	1022:1051	Fermentation of samples resulted in different SCFAS concentrations.					
32331660	8	97	theme	molar	1118:1122	arg1	ratio					1124:1128	the molar ratio	1114:1128	the molar ratio of SCFAS on Hb	1114:1143	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	8	97	theme	molar	1118:1122	arg1	63:18:18					1149:1156	63:18:18	1149:1156	63:18:18	1149:1156	The highest butyric acid production was on AF and OF, while the molar ratio of SCFAS on Hb was 63:18:18 for acetic, propionic and butyric acid, respectively.					
32331660	0	98	theme	sabdariffa	50:59	arg1	calyces					63:69	predigested Hibiscus sabdariffa L calyces	29:69	predigested Hibiscus sabdariffa L calyces	29:69	Changes in gut microbiota in predigested Hibiscus sabdariffa L calyces and Agave (Agave tequilana weber) fructans assessed in a dynamic in vitro model (TIM-2) of the human colon.					
32331660	7	99	theme	samples	1002:1008	arg1	Fermentation					986:997	Fermentation	986:997	Fermentation of samples	986:1008	Fermentation of samples resulted in different SCFAS concentrations.					
32331660	13	100	theme	microbiota	1869:1878	arg1	composition					1880:1890	gut microbiota composition	1865:1890	gut microbiota composition	1865:1890	Evaluation of the changes in metabolites and gut microbiota composition during colonic fermentation in a validated in vitro model provides a complete and reliable view of the potential prebiotic effect of different dietary fibers.					
32331660	5	101	theme	short-chain	644:654	arg1	acids					662:666	short-chain fatty acids	644:666	short-chain fatty acids (SCFAs)	644:674	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	5	101	theme	short-chain	644:654	arg1	SCFAs					669:673	SCFAs	669:673	SCFAs	669:673	Production of short-chain fatty acids (SCFAs), branched-chain fatty acids (BSCFAs) and ammonia was also assessed.					
32331660	6	102	theme	relative	855:862	arg1	abundance					864:872	relative abundance	855:872	relative abundance of genera	855:882	Samples were taken after 0, 24, 48 and 72 h. Principal component (PC) analysis of fermentation metabolites and relative abundance of genera was carried out, and extracted factors were based on eigenvalues >1.0 and explained >60% of variance.					
32331660	4	103	theme	pooled	590:595	arg1	microbiota					609:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota	588:618	A pooled human fecal microbiota was used.					
32412559	7	0	theme	inhibition	1468:1477	arg1	assessment					1447:1456	accurate assessment	1438:1456	accurate assessment of polyST inhibition	1438:1477	In conclusion, an in vitro cell-free assay for accurate assessment of polyST inhibition is described.					
32412559	8	1	theme	full	1695:1698	arg1	inhibition					1712:1721	full competitive inhibition	1695:1721	full competitive inhibition	1695:1721	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	3	2	theme	composition	617:627	arg1	evaluation					593:602	evaluation	593:602	evaluation of metal ion composition	593:627	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	6	3	theme	high	1213:1216	arg1	accuracy					1218:1225	high accuracy	1213:1225	high accuracy	1213:1225	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	2	4	theme	polyST	287:292	arg1	activity					301:308	polyST enzyme activity	287:308	polyST enzyme activity	287:308	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	7	5	dep	in	1409:1410	arg1	vitro					1412:1416	vitro	1412:1416	vitro	1412:1416	In conclusion, an in vitro cell-free assay for accurate assessment of polyST inhibition is described.					
32412559	7	6	theme	accurate	1438:1445	arg1	assessment					1447:1456	accurate assessment	1438:1456	accurate assessment of polyST inhibition	1438:1477	In conclusion, an in vitro cell-free assay for accurate assessment of polyST inhibition is described.					
32412559	7	7	theme	in	1409:1410	arg1	assay					1428:1432	an in vitro cell-free assay	1406:1432	an in vitro cell-free assay for accurate assessment of polyST inhibition	1406:1477	In conclusion, an in vitro cell-free assay for accurate assessment of polyST inhibition is described.					
32412559	6	8	theme	substrate	1283:1291	arg1	concentration					1293:1305	substrate concentration	1283:1305	substrate concentration	1283:1305	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	5	9	theme	observed	914:921	arg1	value					926:930	the experimentally observed Ki value	895:930	the experimentally observed Ki value for CMP, a competitive polyST inhibitor,	895:971	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	4	10	theme	Thorough	771:778	arg1	analysis					780:787	Thorough analysis	771:787	Thorough analysis of each of the assay components	771:819	Thorough analysis of each of the assay components provided a set of optimised conditions.					
32412559	6	11	theme	extensive	1346:1354	arg1	studies					1364:1370	extensive kinetic studies	1346:1370	extensive kinetic studies	1346:1370	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	3	12	theme	acceptor	652:659	arg1	concentrations					661:674	acceptor concentrations	652:674	acceptor concentrations	652:674	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	9	13	theme	anti-metastatic	1796:1810	arg1	targets					1812:1818	important anti-metastatic targets	1786:1818	important anti-metastatic targets in cancer drug discovery	1786:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	6	14	theme	corresponding	1252:1264	arg1	values					1271:1276	the corresponding IC50 values	1248:1276	the corresponding IC50 values with substrate concentration	1248:1305	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	9	15	from	targets	1812:1818	arg1	discovery					1835:1843	cancer drug discovery	1823:1843	cancer drug discovery	1823:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	8	16	theme	assay	1512:1516	arg1	utility					1497:1503	The utility	1493:1503	The utility of the assay for routine identification of potential polyST inhibitors	1493:1574	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	9	17	theme	drug	1830:1833	arg1	discovery					1835:1843	cancer drug discovery	1823:1843	cancer drug discovery	1823:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	8	18	theme	routine	1522:1528	arg1	identification					1530:1543	routine identification	1522:1543	routine identification of potential polyST inhibitors	1522:1574	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	0	19	theme	polysialyltransferase	79:99	arg1	inhibitors					101:110	potential polysialyltransferase inhibitors	69:110	potential polysialyltransferase inhibitors	69:110	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors.					
32412559	1	20	theme	acid	192:195	arg1	polymerisation					163:176	the polymerisation	159:176	the polymerisation of polysialic acid, which plays an important role in tumour metastasis	159:247	The polysialyltransferases (polySTs) catalyse the polymerisation of polysialic acid, which plays an important role in tumour metastasis.					
32412559	8	21	theme	polyST	1558:1563	arg1	inhibitors					1565:1574	potential polyST inhibitors	1548:1574	potential polyST inhibitors	1548:1574	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	2	22	theme	inhibitors	435:444	arg1	identification					372:385	identification	372:385	identification	372:385	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	2	22	theme	inhibitors	435:444	arg1	evaluation					404:413	quantitative evaluation	391:413	quantitative evaluation	391:413	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	9	23	theme	vital	1856:1860	arg1	this					1846:1849	this	1846:1849	this	1846:1849	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	9	23	theme	vital	1856:1860	arg1	tool					1862:1865	a vital tool	1854:1865	a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors	1854:1944	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	2	24	theme	potential	418:426	arg1	inhibitors					435:444	potential polyST inhibitors	418:444	potential polyST inhibitors	418:444	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	4	25	theme	assay	804:808	arg1	components					810:819	the assay components	800:819	the assay components	800:819	Thorough analysis of each of the assay components provided a set of optimised conditions.					
32412559	5	26	theme	predicted	1006:1014	arg1	value					1019:1023	the predicted Ki value	1002:1023	the predicted Ki value	1002:1023	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	9	27	theme	preclinical	1877:1887	arg1	identification					1889:1902	preclinical identification	1877:1902	preclinical identification	1877:1902	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	8	28	theme	quantitative	1602:1613	arg1	measurement					1615:1625	quantitative measurement	1602:1625	quantitative measurement of inhibition	1602:1639	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	5	29	theme	[average	1072:1079	arg1	AFE					1093:1095	AFE	1093:1095	AFE	1093:1095	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	29	theme	[average	1072:1079	arg1	error					1086:1090	the classical Cheng-Prusoff equation [average fold error	1035:1090	the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043]	1035:1105	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	0	30	theme	quantitative	42:53	arg1	evaluation					55:64	quantitative evaluation	42:64	quantitative evaluation	42:64	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors.					
32412559	4	31	theme	optimised	839:847	arg1	conditions					849:858	optimised conditions	839:858	optimised conditions	839:858	Thorough analysis of each of the assay components provided a set of optimised conditions.					
32412559	3	32	theme	comprehensive	534:546	arg1	tolerance					698:706	tolerance	698:706	tolerance	698:706	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	32	theme	comprehensive	534:546	arg1	enzyme					630:635	enzyme	630:635	enzyme	630:635	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	32	theme	comprehensive	534:546	arg1	investigation					548:560	a comprehensive investigation	532:560	a comprehensive investigation of assay conditions	532:580	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	32	theme	comprehensive	534:546	arg1	temperature					677:687	temperature	677:687	temperature	677:687	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	32	theme	comprehensive	534:546	arg1	substrate					638:646	substrate	638:646	substrate	638:646	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	32	theme	comprehensive	534:546	arg1	pH					690:691	pH	690:691	pH	690:691	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	8	33	theme	competitive	1700:1710	arg1	inhibition					1712:1721	full competitive inhibition	1695:1721	full competitive inhibition	1695:1721	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	3	34	theme	metal	607:611	arg1	composition					617:627	metal ion composition	607:627	metal ion composition	607:627	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	9	35	theme	polyST	1928:1933	arg1	inhibitors					1935:1944	novel polyST inhibitors	1922:1944	novel polyST inhibitors	1922:1944	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	5	36	theme	Cheng-Prusoff	1049:1061	arg1	AFE					1093:1095	AFE	1093:1095	AFE	1093:1095	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	36	theme	Cheng-Prusoff	1049:1061	arg1	error					1086:1090	the classical Cheng-Prusoff equation [average fold error	1035:1090	the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043]	1035:1105	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	3	37	theme	conditions	571:580	arg1	tolerance					698:706	tolerance	698:706	tolerance	698:706	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	37	theme	conditions	571:580	arg1	enzyme					630:635	enzyme	630:635	enzyme	630:635	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	37	theme	conditions	571:580	arg1	investigation					548:560	a comprehensive investigation	532:560	a comprehensive investigation of assay conditions	532:580	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	37	theme	conditions	571:580	arg1	temperature					677:687	temperature	677:687	temperature	677:687	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	37	theme	conditions	571:580	arg1	substrate					638:646	substrate	638:646	substrate	638:646	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	37	theme	conditions	571:580	arg1	pH					690:691	pH	690:691	pH	690:691	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	38	theme	known	746:750	arg1	inhibitors					759:768	known polyST inhibitors	746:768	known polyST inhibitors	746:768	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	3	39	theme	ion	613:615	arg1	composition					617:627	metal ion composition	607:627	metal ion composition	607:627	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	5	40	theme	competitive	943:953	arg1	inhibitor					962:970	a competitive polyST inhibitor	941:970	a competitive polyST inhibitor	941:970	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	40	theme	competitive	943:953	arg1	CMP					936:938	CMP	936:938	CMP	936:938	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	41	dep	AFE	1093:1095	arg1	1.043					1100:1104	= 1.043	1098:1104	= 1.043	1098:1104	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	2	42	theme	enzyme	294:299	arg1	activity					301:308	polyST enzyme activity	287:308	polyST enzyme activity	287:308	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	5	43	theme	Ki	923:924	arg1	value					926:930	the experimentally observed Ki value	895:930	the experimentally observed Ki value for CMP, a competitive polyST inhibitor,	895:971	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	9	44	theme	considerable	1734:1745	arg1	interest					1759:1766	the considerable and growing interest	1730:1766	the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery	1730:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	7	45	theme	polyST	1461:1466	arg1	inhibition					1468:1477	polyST inhibition	1461:1477	polyST inhibition	1461:1477	In conclusion, an in vitro cell-free assay for accurate assessment of polyST inhibition is described.					
32412559	9	46	theme	growing	1751:1757	arg1	interest					1759:1766	the considerable and growing interest	1730:1766	the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery	1730:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	6	47	theme	enzyme	1190:1195	arg1	inhibitors					1197:1206	enzyme inhibitors	1190:1206	enzyme inhibitors with high accuracy	1190:1225	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	1	48	theme	important	213:221	arg1	role					223:226	an important role	210:226	an important role	210:226	The polysialyltransferases (polySTs) catalyse the polymerisation of polysialic acid, which plays an important role in tumour metastasis.					
32412559	5	49	theme	polyST	955:960	arg1	inhibitor					962:970	a competitive polyST inhibitor	941:970	a competitive polyST inhibitor	941:970	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	49	theme	polyST	955:960	arg1	CMP					936:938	CMP	936:938	CMP	936:938	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	7	50	theme	cell-free	1418:1426	arg1	assay					1428:1432	an in vitro cell-free assay	1406:1432	an in vitro cell-free assay for accurate assessment of polyST inhibition	1406:1477	In conclusion, an in vitro cell-free assay for accurate assessment of polyST inhibition is described.					
32412559	6	51	theme	kinetic	1356:1362	arg1	studies					1364:1370	extensive kinetic studies	1346:1370	extensive kinetic studies	1346:1370	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	6	52	with	inhibitors	1197:1206	arg1	accuracy					1218:1225	high accuracy	1213:1225	high accuracy	1213:1225	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	9	53	theme	important	1786:1794	arg1	targets					1812:1818	important anti-metastatic targets	1786:1818	important anti-metastatic targets in cancer drug discovery	1786:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	6	54	theme	IC50	1266:1269	arg1	values					1271:1276	the corresponding IC50 values	1248:1276	the corresponding IC50 values with substrate concentration	1248:1305	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	6	55	with	values	1271:1276	arg1	concentration					1293:1305	substrate concentration	1283:1305	substrate concentration	1283:1305	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	3	56	theme	assay	500:504	arg1	development					451:461	The development	447:461	The development of an HPLC-fluorescence-based enzyme assay described within	447:521	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	0	57	theme	potential	69:77	arg1	inhibitors					101:110	potential polysialyltransferase inhibitors	69:110	potential polysialyltransferase inhibitors	69:110	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors.					
32412559	5	58	theme	=	1098:1098	arg1	1.043					1100:1104	= 1.043	1098:1104	= 1.043	1098:1104	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	9	59	theme	cancer	1823:1828	arg1	discovery					1835:1843	cancer drug discovery	1823:1843	cancer drug discovery	1823:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	0	60	theme	inhibitors	101:110	arg1	identification					23:36	identification	23:36	identification	23:36	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors.					
32412559	0	60	theme	inhibitors	101:110	arg1	evaluation					55:64	quantitative evaluation	42:64	quantitative evaluation	42:64	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors.					
32412559	1	61	theme	polysialic	181:190	arg1	acid					192:195	polysialic acid	181:195	polysialic acid	181:195	The polysialyltransferases (polySTs) catalyse the polymerisation of polysialic acid, which plays an important role in tumour metastasis.					
32412559	8	62	theme	potential	1548:1556	arg1	inhibitors					1565:1574	potential polyST inhibitors	1548:1574	potential polyST inhibitors	1548:1574	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	5	63	theme	Ki	1016:1017	arg1	value					1019:1023	the predicted Ki value	1002:1023	the predicted Ki value	1002:1023	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	9	64	from	interest	1759:1766	arg1	polySTs					1775:1781	the polySTs	1771:1781	the polySTs as important anti-metastatic targets in cancer drug discovery	1771:1843	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	8	65	theme	inhibitors	1565:1574	arg1	identification					1530:1543	routine identification	1522:1543	routine identification of potential polyST inhibitors	1522:1574	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	2	66	theme	polyST	428:433	arg1	inhibitors					435:444	potential polyST inhibitors	418:444	potential polyST inhibitors	418:444	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	0	67	theme	efficient	3:11	arg1	assay					13:17	An efficient assay	0:17	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors	0:110	An efficient assay for identification and quantitative evaluation of potential polysialyltransferase inhibitors.					
32412559	4	68	theme	each	792:795	arg1	analysis					780:787	Thorough analysis	771:787	Thorough analysis of each of the assay components	771:819	Thorough analysis of each of the assay components provided a set of optimised conditions.					
32412559	3	69	theme	HPLC-fluorescence-based	469:491	arg1	assay					500:504	an HPLC-fluorescence-based enzyme assay	466:504	an HPLC-fluorescence-based enzyme assay described within	466:521	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	5	70	theme	optimised	873:881	arg1	conditions					883:892	these optimised conditions	867:892	these optimised conditions	867:892	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	71	theme	equation	1063:1070	arg1	AFE					1093:1095	AFE	1093:1095	AFE	1093:1095	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	71	theme	equation	1063:1070	arg1	error					1086:1090	the classical Cheng-Prusoff equation [average fold error	1035:1090	the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043]	1035:1105	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	3	72	theme	enzyme	493:498	arg1	assay					500:504	an HPLC-fluorescence-based enzyme assay	466:504	an HPLC-fluorescence-based enzyme assay described within	466:521	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	8	73	theme	inhibition	1630:1639	arg1	measurement					1615:1625	quantitative measurement	1602:1625	quantitative measurement of inhibition	1602:1639	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	5	74	theme	fold	1081:1084	arg1	AFE					1093:1095	AFE	1093:1095	AFE	1093:1095	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	74	theme	fold	1081:1084	arg1	error					1086:1090	the classical Cheng-Prusoff equation [average fold error	1035:1090	the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043]	1035:1105	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	6	75	theme	medium-throughput	1159:1175	arg1	analysis					1177:1184	medium-throughput analysis	1159:1184	medium-throughput analysis for enzyme inhibitors with high accuracy	1159:1225	These results indicate that this assay can provide medium-throughput analysis for enzyme inhibitors with high accuracy, through determining the corresponding IC50 values with substrate concentration at the KM, without the need to perform extensive kinetic studies for each compound.					
32412559	3	76	theme	assay	565:569	arg1	conditions					571:580	assay conditions	565:580	assay conditions	565:580	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	8	77	theme	inhibition	1712:1721	arg1	assessment					1681:1690	assessment	1681:1690	assessment of full competitive inhibition	1681:1721	The utility of the assay for routine identification of potential polyST inhibitors is demonstrated, allowing quantitative measurement of inhibition to be achieved, and exemplified through assessment of full competitive inhibition.					
32412559	9	78	theme	novel	1922:1926	arg1	inhibitors					1935:1944	novel polyST inhibitors	1922:1944	novel polyST inhibitors	1922:1944	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	2	79	theme	available	335:343	arg1	methodology					323:333	no methodology available	320:343	no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors	320:444	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32412559	1	80	theme	tumour	231:236	arg1	metastasis					238:247	tumour metastasis	231:247	tumour metastasis	231:247	The polysialyltransferases (polySTs) catalyse the polymerisation of polysialic acid, which plays an important role in tumour metastasis.					
32412559	5	81	theme	classical	1039:1047	arg1	AFE					1093:1095	AFE	1093:1095	AFE	1093:1095	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	5	81	theme	classical	1039:1047	arg1	error					1086:1090	the classical Cheng-Prusoff equation [average fold error	1035:1090	the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043]	1035:1105	Under these optimised conditions, the experimentally observed Ki value for CMP, a competitive polyST inhibitor, was strongly correlated with the predicted Ki value, based on the classical Cheng-Prusoff equation [average fold error (AFE) = 1.043].					
32412559	9	82	theme	inhibitors	1935:1944	arg1	identification					1889:1902	preclinical identification	1877:1902	preclinical identification	1877:1902	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	9	82	theme	inhibitors	1935:1944	arg1	evaluation					1908:1917	evaluation	1908:1917	evaluation	1908:1917	Given the considerable and growing interest in the polySTs as important anti-metastatic targets in cancer drug discovery, this is a vital tool to enable preclinical identification and evaluation of novel polyST inhibitors.					
32412559	3	83	theme	polyST	752:757	arg1	inhibitors					759:768	known polyST inhibitors	746:768	known polyST inhibitors	746:768	The development of an HPLC-fluorescence-based enzyme assay described within includes a comprehensive investigation of assay conditions, including evaluation of metal ion composition, enzyme, substrate and acceptor concentrations, temperature, pH, and tolerance to DMSO, followed by validation using known polyST inhibitors.					
32412559	4	84	theme	conditions	849:858	arg1	conditions					849:858	optimised conditions	839:858	optimised conditions	839:858	Thorough analysis of each of the assay components provided a set of optimised conditions.					
32412559	4	84	theme	conditions	849:858	arg1	set					832:834	a set	830:834	a set of optimised conditions	830:858	Thorough analysis of each of the assay components provided a set of optimised conditions.					
32412559	2	85	theme	quantitative	391:402	arg1	evaluation					404:413	quantitative evaluation	391:413	quantitative evaluation	391:413	While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential polyST inhibitors.					
32241442	0	0	theme	soft	65:68	arg1	engineering					77:87	soft tissue engineering	65:87	soft tissue engineering	65:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	4	1	theme	adequate	780:787	arg1	diameter					799:806	adequate mean pore diameter	780:806	adequate mean pore diameter (ca. 60 μm)	780:818	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	1	2	theme	factor-1α	244:252	arg1	release					212:218	the release	208:218	the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	208:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	4	3	dep	diameter	799:806	arg1	60 μm					813:817	ca. 60 μm	809:817	ca. 60 μm	809:817	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	1	4	theme	regenerative	318:329	arg1	medicine					331:338	regenerative medicine	318:338	regenerative medicine	318:338	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	5	theme	biocompatible	152:164	arg1	sponges					174:180	biocompatible aerogel sponges	152:180	biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	152:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	4	6	theme	pore	794:797	arg1	diameter					799:806	adequate mean pore diameter	780:806	adequate mean pore diameter (ca. 60 μm)	780:818	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	0	7	theme	tissue	70:75	arg1	engineering					77:87	soft tissue engineering	65:87	soft tissue engineering	65:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	4	8	theme	mean	789:792	arg1	diameter					799:806	adequate mean pore diameter	780:806	adequate mean pore diameter (ca. 60 μm)	780:818	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	0	9	theme	Composition-properties	90:111	arg1	relationship					113:124	Composition-properties relationship	90:124	Composition-properties relationship	90:124	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	1	10	theme	cancer	343:348	arg1	therapy					350:356	cancer therapy	343:356	cancer therapy (notably for neural tissues)	343:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	11	theme	aerogel	166:172	arg1	sponges					174:180	biocompatible aerogel sponges	152:180	biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	152:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	5	12	theme	slower	968:973	arg1	biodegradation					975:988	slower biodegradation	968:988	slower biodegradation	968:988	Addition of SF yielded sponges with slower biodegradation.					
32241442	1	13	link	cell-derived	231:242	arg1	protein					280:286	a key protein	274:286	a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	274:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	13	link	cell-derived	231:242	arg1	factor-1α					244:252	stromal cell-derived factor-1α	223:252	stromal cell-derived factor-1α (SDF-1α or CXCL12)	223:271	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	0	14	theme	Aerogel	0:6	arg1	sponges					8:14	Aerogel sponges	0:14	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering	0:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	6	15	theme	SDF-1α	1049:1054	arg1	%					1031:1031	75 %	1028:1031	75 %	1028:1031	SF-HA and SF-HA-hep sponges retained 75 % and 93 % of the SDF-1α respectively after 7 days and were found to be cytocompatible in vitro.					
32241442	6	15	theme	SDF-1α	1049:1054	arg1	%					1040:1040	93 %	1037:1040	93 % of the SDF-1α	1037:1054	SF-HA and SF-HA-hep sponges retained 75 % and 93 % of the SDF-1α respectively after 7 days and were found to be cytocompatible in vitro.					
32241442	6	15	theme	SDF-1α	1049:1054	arg1	SDF-1α					1049:1054	the SDF-1α	1045:1054	the SDF-1α	1045:1054	SF-HA and SF-HA-hep sponges retained 75 % and 93 % of the SDF-1α respectively after 7 days and were found to be cytocompatible in vitro.					
32241442	0	16	theme	silk	19:22	arg1	fibroin					24:30	silk fibroin	19:30	silk fibroin	19:30	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	4	17	theme	high	737:740	arg1	%					776:776	∼90 %	772:776	∼90 %	772:776	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	4	17	theme	high	737:740	arg1	degree					751:756	high swelling degree	737:756	high swelling degree	737:756	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	2	18	theme	electron	501:508	arg1	microscopy					510:519	scanning electron microscopy	492:519	scanning electron microscopy	492:519	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	2	19	theme	silk	403:406	arg1	SF					417:418	SF	417:418	SF	417:418	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	2	19	theme	silk	403:406	arg1	fibroin					408:414	silk fibroin	403:414	silk fibroin (SF)	403:419	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	2	20	theme	scanning	492:499	arg1	microscopy					510:519	scanning electron microscopy	492:519	scanning electron microscopy	492:519	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	0	21	dep	relationship	113:124	arg1	sponges					8:14	Aerogel sponges	0:14	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering	0:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	4	22	theme	soft	864:867	arg1	texture					869:875	a soft texture	862:875	a soft texture close to that of the brain (6-13 kPa Young's Modulus)	862:929	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	2	23	theme	acid	436:439	arg1	Miscibility					388:398	Miscibility	388:398	Miscibility of silk fibroin (SF) and hyaluronic acid (HA)	388:444	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	4	24	theme	welcoming	841:849	arg1	cells					851:855	welcoming cells	841:855	welcoming cells	841:855	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	4	25	theme	Young	914:918	arg1	Modulus					922:928	6-13 kPa Young's Modulus	905:928	6-13 kPa Young's Modulus	905:928	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	4	25	theme	Young	914:918	arg1	brain					898:902	the brain	894:902	the brain (6-13 kPa Young's Modulus)	894:929	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	0	26	theme	fibroin	24:30	arg1	sponges					8:14	Aerogel sponges	0:14	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering	0:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	1	27	theme	key	276:278	arg1	protein					280:286	a key protein	274:286	a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	274:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	27	theme	key	276:278	arg1	factor-1α					244:252	stromal cell-derived factor-1α	223:252	stromal cell-derived factor-1α (SDF-1α or CXCL12)	223:271	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	2	28	theme	hyaluronic	425:434	arg1	HA					442:443	HA	442:443	HA	442:443	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	2	28	theme	hyaluronic	425:434	arg1	acid					436:439	hyaluronic acid	425:439	hyaluronic acid (HA)	425:444	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	1	29	theme	neural	371:376	arg1	tissues					378:384	neural tissues	371:384	neural tissues	371:384	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	2	30	theme	fibroin	408:414	arg1	Miscibility					388:398	Miscibility	388:398	Miscibility of silk fibroin (SF) and hyaluronic acid (HA)	388:444	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	0	31	theme	acid	44:47	arg1	sponges					8:14	Aerogel sponges	0:14	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering	0:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	4	32	theme	6-13 kPa	905:912	arg1	Modulus					922:928	6-13 kPa Young's Modulus	905:928	6-13 kPa Young's Modulus	905:928	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	4	32	theme	6-13 kPa	905:912	arg1	brain					898:902	the brain	894:902	the brain (6-13 kPa Young's Modulus)	894:929	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	2	33	theme	processing	533:542	arg1	conditions					544:553	processing conditions	533:553	processing conditions	533:553	Miscibility of silk fibroin (SF) and hyaluronic acid (HA) was investigated by means of fluorescence and scanning electron microscopy to identify processing conditions.					
32241442	6	34	theme	SF-HA-hep	1001:1009	arg1	sponges					1011:1017	SF-HA and SF-HA-hep sponges	991:1017	SF-HA and SF-HA-hep sponges	991:1017	SF-HA and SF-HA-hep sponges retained 75 % and 93 % of the SDF-1α respectively after 7 days and were found to be cytocompatible in vitro.					
32241442	0	35	theme	hyaluronic	33:42	arg1	acid					44:47	hyaluronic acid	33:47	hyaluronic acid	33:47	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	3	36	theme	same	645:648	arg1	structure					653:661	the same 3D structure	641:661	the same 3D structure	641:661	Series of freeze-dried sponges were prepared by associating and cross-linking within the same 3D structure, HA, SF, poly-l-lysine (PLL) and heparin (hep).					
32241442	5	37	theme	SF	944:945	arg1	Addition					932:939	Addition	932:939	Addition of SF	932:945	Addition of SF yielded sponges with slower biodegradation.					
32241442	3	38	theme	3D	650:651	arg1	structure					653:661	the same 3D structure	641:661	the same 3D structure	641:661	Series of freeze-dried sponges were prepared by associating and cross-linking within the same 3D structure, HA, SF, poly-l-lysine (PLL) and heparin (hep).					
32241442	4	39	theme	swelling	742:749	arg1	%					776:776	∼90 %	772:776	∼90 %	772:776	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	4	39	theme	swelling	742:749	arg1	degree					751:756	high swelling degree	737:756	high swelling degree	737:756	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	6	40	theme	SF-HA	991:995	arg1	sponges					1011:1017	SF-HA and SF-HA-hep sponges	991:1017	SF-HA and SF-HA-hep sponges	991:1017	SF-HA and SF-HA-hep sponges retained 75 % and 93 % of the SDF-1α respectively after 7 days and were found to be cytocompatible in vitro.					
32241442	1	41	dep	factor-1α	244:252	arg1	CXCL12					265:270	CXCL12	265:270	CXCL12	265:270	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	41	dep	factor-1α	244:252	arg1	SDF-1α					255:260	SDF-1α	255:260	SDF-1α	255:260	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	4	42	theme	Aerogel	711:717	arg1	sponges					719:725	Aerogel sponges	711:725	Aerogel sponges	711:725	Aerogel sponges presented high swelling degree and porosity (∼90 %), adequate mean pore diameter (ca. 60 μm) and connectivity for welcoming cells, and a soft texture close to that of the brain (6-13 kPa Young's Modulus).					
32241442	5	43	with	sponges	955:961	arg1	biodegradation					975:988	slower biodegradation	968:988	slower biodegradation	968:988	Addition of SF yielded sponges with slower biodegradation.					
32241442	1	44	dep	tissues	378:384	arg1	notably					359:365	notably	359:365	notably	359:365	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	0	45	theme	heparin	53:59	arg1	sponges					8:14	Aerogel sponges	0:14	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering	0:87	Aerogel sponges of silk fibroin, hyaluronic acid and heparin for soft tissue engineering: Composition-properties relationship.					
32241442	3	46	theme	freeze-dried	566:577	arg1	sponges					579:585	freeze-dried sponges	566:585	freeze-dried sponges	566:585	Series of freeze-dried sponges were prepared by associating and cross-linking within the same 3D structure, HA, SF, poly-l-lysine (PLL) and heparin (hep).					
32241442	1	47	theme	stromal	223:229	arg1	protein					280:286	a key protein	274:286	a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	274:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	47	theme	stromal	223:229	arg1	factor-1α					244:252	stromal cell-derived factor-1α	223:252	stromal cell-derived factor-1α (SDF-1α or CXCL12)	223:271	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	3	48	theme	sponges	579:585	arg1	Series					556:561	Series	556:561	Series of freeze-dried sponges	556:585	Series of freeze-dried sponges were prepared by associating and cross-linking within the same 3D structure, HA, SF, poly-l-lysine (PLL) and heparin (hep).					
32241442	1	49	theme	cell-derived	231:242	arg1	protein					280:286	a key protein	274:286	a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues)	274:385	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
32241442	1	49	theme	cell-derived	231:242	arg1	factor-1α					244:252	stromal cell-derived factor-1α	223:252	stromal cell-derived factor-1α (SDF-1α or CXCL12)	223:271	This work aims to design biocompatible aerogel sponges that can host and control the release of stromal cell-derived factor-1α (SDF-1α or CXCL12), a key protein for applications ranging from regenerative medicine to cancer therapy (notably for neural tissues).					
33887293	5	0	theme	as-prepared	743:753	arg1	aerogels					774:781	the as-prepared magnetic composite aerogels	739:781	the as-prepared magnetic composite aerogels	739:781	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	4	1	theme	adsorption	660:669	arg1	properties					671:680	the adsorption properties	656:680	the adsorption properties of the aerogels	656:696	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	6	2	theme	aerogel	1006:1012	arg1	strength					957:964	maximum compress strength	940:964	maximum compress strength	940:964	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	6	2	theme	aerogel	1006:1012	arg1	capacity					981:988	absorption capacity	970:988	absorption capacity	970:988	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	1	3	theme	heavy	247:251	arg1	ions					259:262	heavy metal ions	247:262	heavy metal ions	247:262	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	5	4	theme	magnetic	755:762	arg1	aerogels					774:781	the as-prepared magnetic composite aerogels	739:781	the as-prepared magnetic composite aerogels	739:781	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	5	5	with	water	891:895	arg1	rate					914:917	the removal rate	902:917	the removal rate of 97.5% in 6 h	902:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	1	6	theme	efficient	167:175	arg1	essential					189:197	essential	189:197	essential	189:197	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	1	6	theme	efficient	167:175	arg1	material					177:184	A low-cost, collectable, and efficient material	138:184	A low-cost, collectable, and efficient material	138:184	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	1	7	theme	metal	253:257	arg1	ions					259:262	heavy metal ions	247:262	heavy metal ions	247:262	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	0	8	theme	efficient	113:121	arg1	aerogel					99:105	citric acid cross-linked magnetic aerogel	65:105	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	8	theme	efficient	113:121	arg1	dye					123:125	an efficient dye adsorbent	110:135	an efficient dye adsorbent	110:135	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	6	9	dep	strength	957:964	arg1	The					936:938	The	936:938	The	936:938	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	3	10	theme	citric	501:506	arg1	crosslinker					525:535	the crosslinker	521:535	the crosslinker	521:535	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	3	10	theme	citric	501:506	arg1	CA					514:515	CA	514:515	CA	514:515	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	3	10	theme	citric	501:506	arg1	acid					508:511	citric acid	501:511	citric acid (CA)	501:516	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	4	11	theme	nanoparticle	631:642	arg1	contents					644:651	magnetic Fe3O4 nanoparticle contents	616:651	magnetic Fe3O4 nanoparticle contents	616:651	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	6	12	theme	compress	948:955	arg1	strength					957:964	maximum compress strength	940:964	maximum compress strength	940:964	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	8	13	theme	aerogels	1318:1325	arg1	integrity					1275:1283	long-term integrity	1265:1283	long-term integrity	1265:1283	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	8	13	theme	aerogels	1318:1325	arg1	floatability					1289:1300	floatability	1289:1300	floatability	1289:1300	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	6	14	theme	maximum	940:946	arg1	strength					957:964	maximum compress strength	940:964	maximum compress strength	940:964	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	0	15	theme	adsorbent	127:135	arg1	aerogel					99:105	citric acid cross-linked magnetic aerogel	65:105	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	15	theme	adsorbent	127:135	arg1	dye					123:125	an efficient dye adsorbent	110:135	an efficient dye adsorbent	110:135	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	4	16	theme	aerogels	689:696	arg1	properties					671:680	the adsorption properties	656:680	the adsorption properties of the aerogels	656:696	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	8	17	theme	excellent	1205:1213	arg1	structure					1215:1223	This excellent structure stability and collectability	1200:1252	structure	1215:1223	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	3	18	theme	sodium	450:455	arg1	hydrogel					486:493	sodium carboxymethylcellulose (CMC) hydrogel	450:493	sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels	450:598	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	8	19	from	integrity	1275:1283	arg1	water					1330:1334	water	1330:1334	water	1330:1334	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	5	20	theme	removal	906:912	arg1	rate					914:917	the removal rate	902:917	the removal rate of 97.5% in 6 h	902:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	2	21	theme	aerogel	379:385	arg1	preparation					326:336	the preparation	322:336	the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye	322:406	In this work, we proposed a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye.					
33887293	5	22	theme	porous	791:796	arg1	structure					798:806	porous structure	791:806	porous structure	791:806	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	8	23	theme	long-term	1265:1273	arg1	integrity					1275:1283	long-term integrity	1265:1283	long-term integrity	1265:1283	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	8	24	dep	structure	1215:1223	arg1	stability					1225:1233	stability	1225:1233	stability	1225:1233	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	2	25	theme	magnetic	370:377	arg1	aerogel					379:385	an efficient and collectable magnetic aerogel	341:385	an efficient and collectable magnetic aerogel as adsorbent for dye	341:406	In this work, we proposed a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye.					
33887293	7	26	theme	Fe3O4	1114:1118	arg1	nanoparticles					1120:1132	Fe3O4 nanoparticles	1114:1132	Fe3O4 nanoparticles	1114:1132	Aerogels with Fe3O4 nanoparticles exhibited magnetism which enables the aerogels to easily collect.					
33887293	6	27	theme	nanoparticle	1031:1042	arg1	loading					1044:1050	1 wt% Fe3O4 nanoparticle loading	1019:1050	1 wt% Fe3O4 nanoparticle loading for MB	1019:1057	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	5	28	from	rate	914:917	arg1	h					933:933	6 h	931:933	6 h	931:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	7	29	with	Aerogels	1100:1107	arg1	nanoparticles					1120:1132	Fe3O4 nanoparticles	1114:1132	Fe3O4 nanoparticles	1114:1132	Aerogels with Fe3O4 nanoparticles exhibited magnetism which enables the aerogels to easily collect.					
33887293	2	30	theme	collectable	358:368	arg1	aerogel					379:385	an efficient and collectable magnetic aerogel	341:385	an efficient and collectable magnetic aerogel as adsorbent for dye	341:406	In this work, we proposed a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye.					
33887293	4	31	theme	contents	644:651	arg1	effects					605:611	The effects	601:611	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels	601:696	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	5	32	theme	collectable	840:850	arg1	performance					852:862	collectable performance	840:862	collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h	840:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	6	33	theme	Fe3O4	1025:1029	arg1	loading					1044:1050	1 wt% Fe3O4 nanoparticle loading	1019:1050	1 wt% Fe3O4 nanoparticle loading for MB	1019:1057	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	8	34	from	floatability	1289:1300	arg1	water					1330:1334	water	1330:1334	water	1330:1334	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	5	35	theme	%	926:926	arg1	rate					914:917	the removal rate	902:917	the removal rate of 97.5% in 6 h	902:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	5	36	theme	good	820:823	arg1	adsorption					825:834	good adsorption	820:834	good adsorption	820:834	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	2	37	theme	efficient	344:352	arg1	aerogel					379:385	an efficient and collectable magnetic aerogel	341:385	an efficient and collectable magnetic aerogel as adsorbent for dye	341:406	In this work, we proposed a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye.					
33887293	1	38	dep	dyes	238:241	arg1	pollution					264:272	pollution	264:272	pollution	264:272	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	4	39	from	effects	605:611	arg1	properties					671:680	the adsorption properties	656:680	the adsorption properties of the aerogels	656:696	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	1	40	theme	water	213:217	arg1	pollution					219:227	water pollution	213:227	water pollution	213:227	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	5	41	theme	composite	764:772	arg1	aerogels					774:781	the as-prepared magnetic composite aerogels	739:781	the as-prepared magnetic composite aerogels	739:781	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	4	42	theme	magnetic	616:623	arg1	contents					644:651	magnetic Fe3O4 nanoparticle contents	616:651	magnetic Fe3O4 nanoparticle contents	616:651	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	3	43	theme	freeze-drying	557:569	arg1	technique					571:579	vacuum freeze-drying technique	550:579	vacuum freeze-drying technique to obtain aerogels	550:598	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	6	44	theme	wt	1021:1022	arg1	loading					1044:1050	1 wt% Fe3O4 nanoparticle loading	1019:1050	1 wt% Fe3O4 nanoparticle loading for MB	1019:1057	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	2	45	theme	adsorbent	390:398	arg1	aerogel					379:385	an efficient and collectable magnetic aerogel	341:385	an efficient and collectable magnetic aerogel as adsorbent for dye	341:406	In this work, we proposed a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye.					
33887293	0	46	theme	carboxymethylcellulose	36:57	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	46	theme	carboxymethylcellulose	36:57	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	5	47	theme	blue	878:881	arg1	methylene					868:876	methylene blue	868:881	methylene blue (MB)	868:886	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	5	47	theme	blue	878:881	arg1	MB					884:885	MB	884:885	MB	884:885	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	1	48	dep	such	230:233	arg1	as					235:236	as	235:236	as	235:236	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	6	49	theme	%	1023:1023	arg1	loading					1044:1050	1 wt% Fe3O4 nanoparticle loading	1019:1050	1 wt% Fe3O4 nanoparticle loading for MB	1019:1057	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	0	50	theme	acid	72:75	arg1	aerogel					99:105	citric acid cross-linked magnetic aerogel	65:105	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	50	theme	acid	72:75	arg1	dye					123:125	an efficient dye adsorbent	110:135	an efficient dye adsorbent	110:135	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	3	51	theme	magnetic	413:420	arg1	aerogels					422:429	The magnetic aerogels	409:429	The magnetic aerogels	409:429	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	5	52	from	performance	852:862	arg1	water					891:895	water	891:895	water with the removal rate of 97.5% in 6 h	891:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	3	53	theme	CMC	481:483	arg1	hydrogel					486:493	sodium carboxymethylcellulose (CMC) hydrogel	450:493	sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels	450:598	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	0	54	theme	citric	65:70	arg1	acid					72:75	citric acid	65:75	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	6	55	with	aerogel	1006:1012	arg1	loading					1044:1050	1 wt% Fe3O4 nanoparticle loading	1019:1050	1 wt% Fe3O4 nanoparticle loading for MB	1019:1057	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	3	56	theme	vacuum	550:555	arg1	technique					571:579	vacuum freeze-drying technique	550:579	vacuum freeze-drying technique to obtain aerogels	550:598	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	5	57	from	adsorption	825:834	arg1	water					891:895	water	891:895	water with the removal rate of 97.5% in 6 h	891:933	The results show that the as-prepared magnetic composite aerogels exhibit porous structure and display good adsorption and collectable performance for methylene blue (MB) in water with the removal rate of 97.5% in 6 h.					
33887293	0	58	link	cross-linked	77:88	arg1	aerogel					99:105	citric acid cross-linked magnetic aerogel	65:105	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	58	link	cross-linked	77:88	arg1	dye					123:125	an efficient dye adsorbent	110:135	an efficient dye adsorbent	110:135	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	1	59	theme	low-cost	140:147	arg1	essential					189:197	essential	189:197	essential	189:197	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	1	59	theme	low-cost	140:147	arg1	material					177:184	A low-cost, collectable, and efficient material	138:184	A low-cost, collectable, and efficient material	138:184	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	2	60	theme	novel	303:307	arg1	strategy					309:316	a novel strategy	301:316	a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye	301:406	In this work, we proposed a novel strategy for the preparation of an efficient and collectable magnetic aerogel as adsorbent for dye.					
33887293	8	61	theme	magnetic	1309:1316	arg1	aerogels					1318:1325	the magnetic aerogels	1305:1325	the magnetic aerogels	1305:1325	This excellent structure stability and collectability guarantees long-term integrity and floatability of the magnetic aerogels in water.					
33887293	3	62	theme	carboxymethylcellulose	457:478	arg1	hydrogel					486:493	sodium carboxymethylcellulose (CMC) hydrogel	450:493	sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels	450:598	The magnetic aerogels were prepared from sodium carboxymethylcellulose (CMC) hydrogel using citric acid (CA) as the crosslinker, followed by vacuum freeze-drying technique to obtain aerogels.					
33887293	0	63	theme	magnetic	90:97	arg1	aerogel					99:105	citric acid cross-linked magnetic aerogel	65:105	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	63	theme	magnetic	90:97	arg1	dye					123:125	an efficient dye adsorbent	110:135	an efficient dye adsorbent	110:135	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	6	64	theme	magnetic	997:1004	arg1	aerogel					1006:1012	the magnetic aerogel	993:1012	the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB	993:1057	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
33887293	1	65	theme	collectable	150:160	arg1	essential					189:197	essential	189:197	essential	189:197	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	1	65	theme	collectable	150:160	arg1	material					177:184	A low-cost, collectable, and efficient material	138:184	A low-cost, collectable, and efficient material	138:184	A low-cost, collectable, and efficient material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.					
33887293	4	66	theme	Fe3O4	625:629	arg1	contents					644:651	magnetic Fe3O4 nanoparticle contents	616:651	magnetic Fe3O4 nanoparticle contents	616:651	The effects of magnetic Fe3O4 nanoparticle contents on the adsorption properties of the aerogels were investigated.					
33887293	0	67	theme	cross-linked	77:88	arg1	aerogel					99:105	citric acid cross-linked magnetic aerogel	65:105	citric acid cross-linked magnetic aerogel	65:105	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	0	67	theme	cross-linked	77:88	arg1	dye					123:125	an efficient dye adsorbent	110:135	an efficient dye adsorbent	110:135	Preparation and characterization of carboxymethylcellulose based citric acid cross-linked magnetic aerogel as an efficient dye adsorbent.					
33887293	6	68	theme	absorption	970:979	arg1	capacity					981:988	absorption capacity	970:988	absorption capacity	970:988	The maximum compress strength and absorption capacity of the magnetic aerogel with 1 wt% Fe3O4 nanoparticle loading for MB is 0.13 MPa and 83.6 mg/g, respectively.					
34856502	0	0	theme	m-aminophenylboronic	89:108	arg1	gel					123:125	m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	89:196	gel	123:125	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	4	1	theme	secondary	581:589	arg1	profiling					602:610	secondary metabolite profiling	581:610	secondary metabolite profiling	581:610	Given this chemical diversity there is a need to develop selective and sensitive methods for secondary metabolite profiling.					
34856502	7	2	theme	m-aminophenylboronic	1243:1262	arg1	gel					1269:1271	m-aminophenylboronic acid gel	1243:1271	m-aminophenylboronic acid gel	1243:1271	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	6	3	theme	isomer	1017:1022	arg1	confirmation					974:985	the confirmation	970:985	the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3)	970:1049	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	8	4	theme	boronate	1348:1355	arg1	procedure					1366:1374	this non-traditional boronate affinity procedure	1327:1374	this non-traditional boronate affinity procedure	1327:1374	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	0	5	from	extraction	10:19	arg1	silvae					76:81	Gambierdiscus silvae	62:81	Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	62:196	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	7	6	theme	acid	1264:1267	arg1	gel					1269:1271	m-aminophenylboronic acid gel	1243:1271	m-aminophenylboronic acid gel	1243:1271	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	5	7	theme	silvae	681:686	arg1	strain					657:662	a cultured Caribbean strain	636:662	a cultured Caribbean strain of Gambierdiscus silvae	636:686	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	5	8	used	used	631:634	arg2	we					628:629	we	628:629	we	628:629	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	7	9	contain	contained	1128:1136	arg2	vic-diol					1151:1158	one reactive vic-diol	1138:1158	one reactive vic-diol	1138:1158	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	7	9	contain	contained	1128:1136	arg1	gambierones					1111:1121	the gambierones	1107:1121	the gambierones	1107:1121	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	7	10	theme	procedure	1227:1235	arg1	development					1189:1199	the development	1185:1199	the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform	1185:1318	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	0	11	theme	acid-agarose	110:121	arg1	gel					123:125	m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	89:196	gel	123:125	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	4	12	theme	sensitive	559:567	arg1	methods					569:575	selective and sensitive methods	545:575	selective and sensitive methods for secondary metabolite profiling	545:610	Given this chemical diversity there is a need to develop selective and sensitive methods for secondary metabolite profiling.					
34856502	8	13	attach	presence	1403:1410	arg2	ethers					1446:1451	additional sulfated polycyclic ethers	1415:1451	additional sulfated polycyclic ethers	1415:1451	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	8	13	attach	presence	1403:1410	arg1	fraction					1491:1498	the gambierone-containing vic-diol fraction	1456:1498	the gambierone-containing vic-diol fraction	1456:1498	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	4	14	theme	chemical	499:506	arg1	diversity					508:516	this chemical diversity	494:516	this chemical diversity	494:516	Given this chemical diversity there is a need to develop selective and sensitive methods for secondary metabolite profiling.					
34856502	0	15	theme	liquid	131:136	arg1	detection					188:196	m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	89:196	detection	188:196	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	6	16	theme	novel	1011:1015	arg1	isomer					1017:1022	a novel isomer	1009:1022	a novel isomer of 44-methylgambierone (3)	1009:1049	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	5	17	theme	preparation	706:716	arg1	strategies					731:740	sample preparation and analysis strategies	699:740	sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites	699:801	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	5	18	theme	sample	699:704	arg1	preparation					706:716	sample preparation	699:716	sample preparation	699:716	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	10	19	theme	structural	1851:1860	arg1	studies					1899:1905	structural, toxicological and biotransformation studies	1851:1905	structural, toxicological and biotransformation studies	1851:1905	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	0	20	theme	metabolites	47:57	arg1	extraction					10:19	Selective extraction	0:19	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.	0:197	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	3	21	theme	complex	328:334	arg1	profiles					357:364	complex secondary metabolite profiles	328:364	complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones	328:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	21	theme	complex	328:334	arg1	maitotoxins					389:399	maitotoxins	389:399	maitotoxins	389:399	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	21	theme	complex	328:334	arg1	gambierones					406:416	gambierones	406:416	gambierones	406:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	21	theme	complex	328:334	arg1	ciguatoxins					376:386	ciguatoxins	376:386	ciguatoxins	376:386	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	5	22	theme	Caribbean	647:655	arg1	strain					657:662	a cultured Caribbean strain	636:662	a cultured Caribbean strain of Gambierdiscus silvae	636:686	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	8	23	theme	other	1520:1524	arg1	contaminants					1526:1537	other contaminants	1520:1537	other contaminants	1520:1537	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	6	24	theme	cellular	813:820	arg1	extract					822:828	A pooled cellular extract	804:828	A pooled cellular extract	804:828	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	9	25	theme	Gambierdiscus	1615:1627	arg1	collections					1600:1610	collections	1600:1610	collections of Gambierdiscus and other benthic algae	1600:1651	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	10	26	theme	selective	1738:1746	arg1	procedure					1759:1767	The selective extraction procedure	1734:1767	The selective extraction procedure	1734:1767	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	5	27	theme	analysis	722:729	arg1	strategies					731:740	sample preparation and analysis strategies	699:740	sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites	699:801	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	6	28	theme	pooled	806:811	arg1	extract					822:828	A pooled cellular extract	804:828	A pooled cellular extract	804:828	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	0	29	theme	Selective	0:8	arg1	extraction					10:19	Selective extraction	0:19	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.	0:197	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	7	30	theme	selective	1206:1214	arg1	procedure					1227:1235	a selective extraction procedure	1204:1235	a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform	1204:1318	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	9	31	theme	other	1633:1637	arg1	algae					1647:1651	other benthic algae	1633:1651	other benthic algae	1633:1651	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	0	32	theme	gambierone	24:33	arg1	extraction					10:19	Selective extraction	0:19	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.	0:197	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	10	33	from	step	1796:1799	arg1	isolation					1808:1816	the isolation	1804:1816	the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies	1804:1905	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	0	34	theme	mass	169:172	arg1	detection					188:196	m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	89:196	detection	188:196	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	7	35	theme	extract	1069:1075	arg1	Treatment					1052:1060	Treatment	1052:1060	Treatment of the extract with periodate	1052:1090	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	7	36	theme	extraction	1216:1225	arg1	procedure					1227:1235	a selective extraction procedure	1204:1235	a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform	1204:1318	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	8	37	theme	additional	1415:1424	arg1	ethers					1446:1451	additional sulfated polycyclic ethers	1415:1451	additional sulfated polycyclic ethers	1415:1451	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	7	38	theme	solvent	1301:1307	arg1	chloroform					1309:1318	the non-aqueous binding solvent chloroform	1277:1318	the non-aqueous binding solvent chloroform	1277:1318	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	7	39	with	Treatment	1052:1060	arg1	periodate					1082:1090	periodate	1082:1090	periodate	1082:1090	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	0	40	theme	chromatography-high-resolution	138:167	arg1	detection					188:196	m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	89:196	detection	188:196	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	10	41	theme	extraction	1748:1757	arg1	procedure					1759:1767	The selective extraction procedure	1734:1767	The selective extraction procedure	1734:1767	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	9	42	theme	algae	1647:1651	arg1	collections					1600:1610	collections	1600:1610	collections of Gambierdiscus and other benthic algae	1600:1651	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	0	43	theme	related	39:45	arg1	metabolites					47:57	related metabolites	39:57	related metabolites	39:57	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	8	44	theme	ethers	1446:1451	arg1	presence					1403:1410	the presence	1399:1410	the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction	1399:1498	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	3	45	theme	secondary	336:344	arg1	profiles					357:364	complex secondary metabolite profiles	328:364	complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones	328:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	45	theme	secondary	336:344	arg1	maitotoxins					389:399	maitotoxins	389:399	maitotoxins	389:399	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	45	theme	secondary	336:344	arg1	gambierones					406:416	gambierones	406:416	gambierones	406:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	45	theme	secondary	336:344	arg1	ciguatoxins					376:386	ciguatoxins	376:386	ciguatoxins	376:386	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	10	46	theme	polyethers	1836:1845	arg1	isolation					1808:1816	the isolation	1804:1816	the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies	1804:1905	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	8	47	theme	polycyclic	1435:1444	arg1	ethers					1446:1451	additional sulfated polycyclic ethers	1415:1451	additional sulfated polycyclic ethers	1415:1451	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	7	48	theme	non-aqueous	1281:1291	arg1	chloroform					1309:1318	the non-aqueous binding solvent chloroform	1277:1318	the non-aqueous binding solvent chloroform	1277:1318	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	10	49	theme	biotransformation	1881:1897	arg1	studies					1899:1905	structural, toxicological and biotransformation studies	1851:1905	structural, toxicological and biotransformation studies	1851:1905	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	3	50	theme	metabolite	346:355	arg1	profiles					357:364	complex secondary metabolite profiles	328:364	complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones	328:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	50	theme	metabolite	346:355	arg1	maitotoxins					389:399	maitotoxins	389:399	maitotoxins	389:399	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	50	theme	metabolite	346:355	arg1	gambierones					406:416	gambierones	406:416	gambierones	406:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	50	theme	metabolite	346:355	arg1	ciguatoxins					376:386	ciguatoxins	376:386	ciguatoxins	376:386	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	4	51	theme	selective	545:553	arg1	methods					569:575	selective and sensitive methods	545:575	selective and sensitive methods for secondary metabolite profiling	545:610	Given this chemical diversity there is a need to develop selective and sensitive methods for secondary metabolite profiling.					
34856502	7	52	theme	binding	1293:1299	arg1	chloroform					1309:1318	the non-aqueous binding solvent chloroform	1277:1318	the non-aqueous binding solvent chloroform	1277:1318	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	8	53	from	presence	1403:1410	arg1	fraction					1491:1498	the gambierone-containing vic-diol fraction	1456:1498	the gambierone-containing vic-diol fraction	1456:1498	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	8	54	theme	sulfated	1426:1433	arg1	ethers					1446:1451	additional sulfated polycyclic ethers	1415:1451	additional sulfated polycyclic ethers	1415:1451	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	5	55	theme	secondary	781:789	arg1	metabolites					791:801	secondary metabolites	781:801	secondary metabolites	781:801	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	6	56	theme	gambierone	990:999	arg1	confirmation					974:985	the confirmation	970:985	the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3)	970:1049	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	7	57	theme	reactive	1142:1149	arg1	vic-diol					1151:1158	one reactive vic-diol	1138:1158	one reactive vic-diol	1138:1158	Treatment of the extract with periodate confirmed that the gambierones each contained one reactive vic-diol, which was exploited for the development of a selective extraction procedure using m-aminophenylboronic acid gel and the non-aqueous binding solvent chloroform.					
34856502	9	58	theme	additional	1664:1673	arg1	characterization					1684:1699	additional chemical characterization	1664:1699	additional chemical characterization of gambierone-related compounds	1664:1731	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	0	59	theme	spectrometric	174:186	arg1	detection					188:196	m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection	89:196	detection	188:196	Selective extraction of gambierone and related metabolites in Gambierdiscus silvae using m-aminophenylboronic acid-agarose gel and liquid chromatography-high-resolution mass spectrometric detection.					
34856502	9	60	theme	gambierone-related	1704:1721	arg1	compounds					1723:1731	gambierone-related compounds	1704:1731	gambierone-related compounds	1704:1731	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	6	61	theme	44-methylgambierone	1027:1045	arg1	isomer					1017:1022	a novel isomer	1009:1022	a novel isomer of 44-methylgambierone (3)	1009:1049	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	6	61	theme	44-methylgambierone	1027:1045	arg1	gambierone					990:999	gambierone (1)	990:1003	gambierone (1)	990:1003	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	2	62	theme	ciguatera	281:289	arg1	poisoning					291:299	ciguatera poisoning	281:299	ciguatera poisoning	281:299	are epi-benthic dinoflagellates that have been associated with ciguatera poisoning.					
34856502	6	63	theme	mass	890:893	arg1	LC-HRMS					909:915	LC-HRMS	909:915	LC-HRMS	909:915	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	6	63	theme	mass	890:893	arg1	spectrometry					895:906	liquid chromatography-high-resolution mass spectrometry	852:906	liquid chromatography-high-resolution mass spectrometry (LC-HRMS)	852:916	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	2	64	theme	epi-benthic	222:232	arg1	dinoflagellates					234:248	epi-benthic dinoflagellates	222:248	epi-benthic dinoflagellates that have been associated with ciguatera poisoning	222:299	are epi-benthic dinoflagellates that have been associated with ciguatera poisoning.					
34856502	10	65	theme	sulfated	1827:1834	arg1	polyethers					1836:1845	these sulfated polyethers	1821:1845	these sulfated polyethers for structural, toxicological and biotransformation studies	1821:1905	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	6	66	theme	ciguatoxin-related	922:939	arg1	compounds					941:949	ciguatoxin-related compounds	922:949	ciguatoxin-related compounds	922:949	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	5	67	theme	cultured	638:645	arg1	strain					657:662	a cultured Caribbean strain	636:662	a cultured Caribbean strain of Gambierdiscus silvae	636:686	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	8	68	theme	gambierone-containing	1460:1480	arg1	fraction					1491:1498	the gambierone-containing vic-diol fraction	1456:1498	the gambierone-containing vic-diol fraction	1456:1498	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	9	69	theme	developed	1557:1565	arg1	tools					1567:1571	The developed tools	1553:1571	The developed tools	1553:1571	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	5	70	contain	containing	770:779	arg1	vic-diol					761:768	vic-diol	761:768	vic-diol containing secondary metabolites	761:801	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	5	70	contain	containing	770:779	arg2	metabolites					791:801	secondary metabolites	781:801	secondary metabolites	781:801	In this study, we used a cultured Caribbean strain of Gambierdiscus silvae to develop sample preparation and analysis strategies for characterizing vic-diol containing secondary metabolites.					
34856502	6	71	theme	chromatography-high-resolution	859:888	arg1	LC-HRMS					909:915	LC-HRMS	909:915	LC-HRMS	909:915	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	6	71	theme	chromatography-high-resolution	859:888	arg1	spectrometry					895:906	liquid chromatography-high-resolution mass spectrometry	852:906	liquid chromatography-high-resolution mass spectrometry (LC-HRMS)	852:916	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	6	72	theme	liquid	852:857	arg1	LC-HRMS					909:915	LC-HRMS	909:915	LC-HRMS	909:915	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	6	72	theme	liquid	852:857	arg1	spectrometry					895:906	liquid chromatography-high-resolution mass spectrometry	852:906	liquid chromatography-high-resolution mass spectrometry (LC-HRMS)	852:916	A pooled cellular extract was first screened by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) for ciguatoxin-related compounds, which resulted in the confirmation of gambierone (1) and a novel isomer of 44-methylgambierone (3).					
34856502	9	73	theme	compounds	1723:1731	arg1	characterization					1684:1699	additional chemical characterization	1664:1699	additional chemical characterization of gambierone-related compounds	1664:1731	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	8	74	theme	affinity	1357:1364	arg1	procedure					1366:1374	this non-traditional boronate affinity procedure	1327:1374	this non-traditional boronate affinity procedure	1327:1374	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	10	75	theme	toxicological	1863:1875	arg1	studies					1899:1905	structural, toxicological and biotransformation studies	1851:1905	structural, toxicological and biotransformation studies	1851:1905	The selective extraction procedure may also prove useful as a step in the isolation of these sulfated polyethers for structural, toxicological and biotransformation studies.					
34856502	9	76	theme	benthic	1639:1645	arg1	algae					1647:1651	other benthic algae	1633:1651	other benthic algae	1633:1651	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	9	77	theme	chemical	1675:1682	arg1	characterization					1684:1699	additional chemical characterization	1664:1699	additional chemical characterization of gambierone-related compounds	1664:1731	The developed tools could be applied to screen collections of Gambierdiscus and other benthic algae to provide additional chemical characterization of gambierone-related compounds.					
34856502	3	78	contain	have	323:326	arg2	maitotoxins					389:399	maitotoxins	389:399	maitotoxins	389:399	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	78	contain	have	323:326	arg2	gambierones					406:416	gambierones	406:416	gambierones	406:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	78	contain	have	323:326	arg2	ciguatoxins					376:386	ciguatoxins	376:386	ciguatoxins	376:386	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	78	contain	have	323:326	arg1	microalgae					308:317	These microalgae	302:317	These microalgae	302:317	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	3	78	contain	have	323:326	arg2	profiles					357:364	complex secondary metabolite profiles	328:364	complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones	328:416	These microalgae can have complex secondary metabolite profiles including ciguatoxins, maitotoxins, and gambierones, with varying compositions and toxicities across species and strains.					
34856502	4	79	theme	metabolite	591:600	arg1	profiling					602:610	secondary metabolite profiling	581:610	secondary metabolite profiling	581:610	Given this chemical diversity there is a need to develop selective and sensitive methods for secondary metabolite profiling.					
34856502	8	80	theme	non-traditional	1332:1346	arg1	procedure					1366:1374	this non-traditional boronate affinity procedure	1327:1374	this non-traditional boronate affinity procedure	1327:1374	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34856502	8	81	theme	vic-diol	1482:1489	arg1	fraction					1491:1498	the gambierone-containing vic-diol fraction	1456:1498	the gambierone-containing vic-diol fraction	1456:1498	Using this non-traditional boronate affinity procedure, LC-HRMS also revealed the presence of additional sulfated polycyclic ethers in the gambierone-containing vic-diol fraction, while pigments and other contaminants were removed.					
34944401	6	0	theme	NCgl2761	870:877	arg1	Deletion					835:842	Deletion	835:842	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis)	835:917	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	8	1	theme	ΔtmaT	1271:1275	arg1	mutants					1294:1300	the ΔtmaT, ΔmtrP and ΔmmpA mutants	1267:1300	the ΔtmaT, ΔmtrP and ΔmmpA mutants	1267:1300	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	9	2	theme	hTMCM	1452:1456	arg1	acetylation					1414:1424	acetylation	1414:1424	acetylation	1414:1424	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	2	theme	hTMCM	1452:1456	arg1	transport					1439:1447	membrane transport	1430:1447	membrane transport	1430:1447	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	6	3	theme	gene	865:868	arg1	NCgl2761					870:877	the C. glutamicum gene NCgl2761	847:877	the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis)	847:917	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	9	4	dep	acetylation	1414:1424	arg1	the					1410:1412	the	1410:1412	the	1410:1412	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	2	5	theme	membrane	325:332	arg1	leaflet					304:310	the cytoplasmic leaflet	288:310	the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm	288:421	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	0	6	theme	Lipids	89:94	arg1	Transport					66:74	Efficient Surface Transport	48:74	Efficient Surface Transport of Trehalose Lipids in Corynebacterineae	48:115	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	9	7	dep	proteins	1483:1490	arg1	TmaT					1508:1511	TmaT	1508:1511	TmaT	1508:1511	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	7	dep	proteins	1483:1490	arg1	MtrP					1499:1502	MtrP	1499:1502	MtrP	1499:1502	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	7	dep	proteins	1483:1490	arg1	MmpA					1493:1496	MmpA	1493:1496	MmpA	1493:1496	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	7	dep	proteins	1483:1490	arg1	proteins					1483:1490	multiple proteins	1474:1490	multiple proteins: MmpA, MtrP and TmaT	1474:1511	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	8	theme	compensatory	1575:1586	arg1	changes					1588:1594	global, potentially compensatory changes	1555:1594	changes	1588:1594	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	1	9	dep	Mycobacterium	155:167	arg1	the					144:146	the	144:146	the	144:146	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	3	10	theme	monohydroxycorynomycolate	510:534	arg1	acetylation					485:495	acetylation	485:495	acetylation of trehalose monohydroxycorynomycolate (hTMCM)	485:542	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	6	11	theme	hTMCM	953:957	arg1	synthesis					929:937	synthesis	929:937	synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane	929:1027	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	0	12	theme	Trehalose	79:87	arg1	Lipids					89:94	Trehalose Lipids	79:94	Trehalose Lipids	79:94	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	6	13	theme	C.	851:852	arg1	NCgl2761					870:877	the C. glutamicum gene NCgl2761	847:877	the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis)	847:917	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	9	14	theme	multiple	1474:1481	arg1	TmaT					1508:1511	TmaT	1508:1511	TmaT	1508:1511	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	14	theme	multiple	1474:1481	arg1	MtrP					1499:1502	MtrP	1499:1502	MtrP	1499:1502	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	14	theme	multiple	1474:1481	arg1	MmpA					1493:1496	MmpA	1493:1496	MmpA	1493:1496	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	9	14	theme	multiple	1474:1481	arg1	proteins					1483:1490	multiple proteins	1474:1490	multiple proteins: MmpA, MtrP and TmaT	1474:1511	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	6	15	theme	outer	1106:1110	arg1	dihydroxycorynomycolate					1064:1086	trehalose dihydroxycorynomycolate	1054:1086	trehalose dihydroxycorynomycolate (h2TDCM)	1054:1095	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	15	theme	outer	1106:1110	arg1	glycolipid					1121:1130	a major outer membrane glycolipid	1098:1130	a major outer membrane glycolipid	1098:1130	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	16	theme	acetylated	942:951	arg1	AcTMCM					960:965	AcTMCM	960:965	AcTMCM	960:965	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	16	theme	acetylated	942:951	arg1	hTMCM					953:957	acetylated hTMCM	942:957	acetylated hTMCM (AcTMCM)	942:966	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	3	17	theme	Corynebacterium	442:456	arg1	glutamicum					458:467	Corynebacterium glutamicum	442:467	Corynebacterium glutamicum	442:467	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	4	18	theme	methyltransferase	706:722	arg1	presence					683:690	the presence	679:690	the presence of a putative methyltransferase, MtrP	679:728	Acetylation is mediated by the membrane acetyltransferase, TmaT, and is dependent on the presence of a putative methyltransferase, MtrP.					
34944401	2	19	theme	fatty	257:261	arg1	acids					263:267	These very long chain fatty acids	235:267	These very long chain fatty acids	235:267	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	5	20	theme	hTMCM	828:832	arg1	acetylation					790:800	acetylation	790:800	acetylation	790:800	Here, we identify a third protein that is required for the acetylation and membrane transport of hTMCM.					
34944401	5	20	theme	hTMCM	828:832	arg1	transport					815:823	membrane transport	806:823	membrane transport	806:823	Here, we identify a third protein that is required for the acetylation and membrane transport of hTMCM.					
34944401	1	21	theme	Mycobacterium	155:167	arg1	bacteria					132:139	bacteria	132:139	bacteria of the genera Mycobacterium and Corynebacterium	132:187	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	6	22	dep	NCgl2761	870:877	arg1	Rv0226c					880:886	Rv0226c	880:886	Rv0226c	880:886	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	3	23	from	studies	431:437	arg1	glutamicum					458:467	Corynebacterium glutamicum	442:467	Corynebacterium glutamicum	442:467	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	2	24	theme	chain	251:255	arg1	acids					263:267	These very long chain fatty acids	235:267	These very long chain fatty acids	235:267	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	8	25	from	changes	1337:1343	arg1	composition					1371:1381	overall membrane lipid composition	1348:1381	overall membrane lipid composition	1348:1381	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	6	26	theme	inner	1014:1018	arg1	membrane					1020:1027	the inner membrane	1010:1027	the inner membrane	1010:1027	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	27	theme	major	1100:1104	arg1	dihydroxycorynomycolate					1064:1086	trehalose dihydroxycorynomycolate	1054:1086	trehalose dihydroxycorynomycolate (h2TDCM)	1054:1095	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	27	theme	major	1100:1104	arg1	glycolipid					1121:1130	a major outer membrane glycolipid	1098:1130	a major outer membrane glycolipid	1098:1130	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	2	28	theme	cytoplasmic	292:302	arg1	leaflet					304:310	the cytoplasmic leaflet	288:310	the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm	288:421	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	7	29	theme	hTMCM	1211:1215	arg1	ratio					1224:1228	the hTMCM:h2TDCM ratio	1207:1228	the hTMCM:h2TDCM ratio	1207:1228	Complementation with the NCgl2761 gene, designated here as mmpA, restored the hTMCM:h2TDCM ratio.					
34944401	1	30	theme	Corynebacterium	173:187	arg1	bacteria					132:139	bacteria	132:139	bacteria of the genera Mycobacterium and Corynebacterium	132:187	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	9	31	from	changes	1588:1594	arg1	composition					1603:1613	the composition	1599:1613	the composition of inner and outer membranes	1599:1642	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	4	32	theme	putative	697:704	arg1	MtrP					725:728	MtrP	725:728	MtrP	725:728	Acetylation is mediated by the membrane acetyltransferase, TmaT, and is dependent on the presence of a putative methyltransferase, MtrP.					
34944401	4	32	theme	putative	697:704	arg1	methyltransferase					706:722	a putative methyltransferase	695:722	a putative methyltransferase	695:722	Acetylation is mediated by the membrane acetyltransferase, TmaT, and is dependent on the presence of a putative methyltransferase, MtrP.					
34944401	8	33	theme	ΔmmpA	1288:1292	arg1	mutants					1294:1300	the ΔtmaT, ΔmtrP and ΔmmpA mutants	1267:1300	the ΔtmaT, ΔmtrP and ΔmmpA mutants	1267:1300	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	2	34	theme	prior	339:343	arg1	IM					335:336	IM	335:336	IM	335:336	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	2	34	theme	prior	339:343	arg1	membrane					325:332	the inner membrane	315:332	the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm	315:421	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	0	35	theme	Conserved	8:16	arg1	Protein					27:33	a Conserved Membrane Protein	6:33	a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae	6:115	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	8	36	theme	Comprehensive	1231:1243	arg1	analysis					1255:1262	Comprehensive lipidomic analysis	1231:1262	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants	1231:1300	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	8	37	theme	similar	1322:1328	arg1	changes					1337:1343	strikingly similar global changes	1311:1343	strikingly similar global changes in overall membrane lipid composition	1311:1381	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	7	38	with	Complementation	1133:1147	arg1	gene					1167:1170	the NCgl2761 gene	1154:1170	the NCgl2761 gene	1154:1170	Complementation with the NCgl2761 gene, designated here as mmpA, restored the hTMCM:h2TDCM ratio.					
34944401	1	39	theme	high	197:200	arg1	levels					202:207	high levels	197:207	high levels of (coryno)mycolic acids	197:232	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	8	40	theme	mutants	1294:1300	arg1	analysis					1255:1262	Comprehensive lipidomic analysis	1231:1262	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants	1231:1300	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	6	41	theme	membrane	1112:1119	arg1	dihydroxycorynomycolate					1064:1086	trehalose dihydroxycorynomycolate	1054:1086	trehalose dihydroxycorynomycolate (h2TDCM)	1054:1095	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	41	theme	membrane	1112:1119	arg1	glycolipid					1121:1130	a major outer membrane glycolipid	1098:1130	a major outer membrane glycolipid	1098:1130	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	1	42	contain	contain	189:195	arg1	walls					123:127	Cell walls	118:127	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium	118:187	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	1	42	contain	contain	189:195	arg2	levels					202:207	high levels	197:207	high levels of (coryno)mycolic acids	197:232	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	1	43	theme	genera	148:153	arg1	Mycobacterium					155:167	genera Mycobacterium	148:167	genera Mycobacterium	148:167	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	3	44	theme	Recent	424:429	arg1	studies					431:437	Recent studies	424:437	Recent studies on Corynebacterium glutamicum	424:467	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	7	45	theme	h2TDCM	1217:1222	arg1	ratio					1224:1228	the hTMCM:h2TDCM ratio	1207:1228	the hTMCM:h2TDCM ratio	1207:1228	Complementation with the NCgl2761 gene, designated here as mmpA, restored the hTMCM:h2TDCM ratio.					
34944401	9	46	theme	outer	1628:1632	arg1	membranes					1634:1642	inner and outer membranes	1618:1642	inner and outer membranes	1618:1642	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	0	47	from	Transport	66:74	arg1	Corynebacterineae					99:115	Corynebacterineae	99:115	Corynebacterineae	99:115	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	2	48	dep	disaccharide	367:378	arg1	the					363:365	the	363:365	the	363:365	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	9	49	from	defects	1523:1529	arg1	process					1539:1545	this process	1534:1545	this process	1534:1545	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	4	50	theme	membrane	625:632	arg1	TmaT					653:656	TmaT	653:656	TmaT	653:656	Acetylation is mediated by the membrane acetyltransferase, TmaT, and is dependent on the presence of a putative methyltransferase, MtrP.					
34944401	4	50	theme	membrane	625:632	arg1	acetyltransferase					634:650	the membrane acetyltransferase	621:650	the membrane acetyltransferase	621:650	Acetylation is mediated by the membrane acetyltransferase, TmaT, and is dependent on the presence of a putative methyltransferase, MtrP.					
34944401	0	51	theme	Membrane	18:25	arg1	Protein					27:33	a Conserved Membrane Protein	6:33	a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae	6:115	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	9	52	theme	membranes	1634:1642	arg1	composition					1603:1613	the composition	1599:1613	the composition of inner and outer membranes	1599:1642	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	3	53	theme	inner	578:582	arg1	membrane					584:591	the inner membrane	574:591	the inner membrane	574:591	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	5	54	theme	membrane	806:813	arg1	transport					815:823	membrane transport	806:823	membrane transport	806:823	Here, we identify a third protein that is required for the acetylation and membrane transport of hTMCM.					
34944401	5	55	theme	third	751:755	arg1	protein					757:763	a third protein	749:763	a third protein that is required for the acetylation and membrane transport of hTMCM	749:832	Here, we identify a third protein that is required for the acetylation and membrane transport of hTMCM.					
34944401	6	56	theme	Mycobacterium	891:903	arg1	tuberculosis					905:916	Mycobacterium tuberculosis	891:916	Mycobacterium tuberculosis	891:916	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	8	57	theme	ΔmtrP	1278:1282	arg1	mutants					1294:1300	the ΔtmaT, ΔmtrP and ΔmmpA mutants	1267:1300	the ΔtmaT, ΔmtrP and ΔmmpA mutants	1267:1300	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	0	58	dep	Protein	27:33	arg1	MmpA					0:3	MmpA	0:3	MmpA	0:3	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	2	59	theme	inner	319:323	arg1	IM					335:336	IM	335:336	IM	335:336	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	2	59	theme	inner	319:323	arg1	membrane					325:332	the inner membrane	315:332	the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm	315:421	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	8	60	theme	overall	1348:1354	arg1	composition					1371:1381	overall membrane lipid composition	1348:1381	overall membrane lipid composition	1348:1381	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	9	61	theme	inner	1618:1622	arg1	membranes					1634:1642	inner and outer membranes	1618:1642	inner and outer membranes	1618:1642	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	7	62	theme	NCgl2761	1158:1165	arg1	gene					1167:1170	the NCgl2761 gene	1154:1170	the NCgl2761 gene	1154:1170	Complementation with the NCgl2761 gene, designated here as mmpA, restored the hTMCM:h2TDCM ratio.					
34944401	1	63	theme	Cell	118:121	arg1	walls					123:127	Cell walls	118:127	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium	118:187	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	8	64	theme	lipid	1365:1369	arg1	composition					1371:1381	overall membrane lipid composition	1348:1381	overall membrane lipid composition	1348:1381	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	9	65	theme	global	1555:1560	arg1	changes					1588:1594	global, potentially compensatory changes	1555:1594	changes	1588:1594	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	6	66	from	accumulation	985:996	arg1	membrane					1020:1027	the inner membrane	1010:1027	the inner membrane	1010:1027	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	2	67	theme	long	246:249	arg1	acids					263:267	These very long chain fatty acids	235:267	These very long chain fatty acids	235:267	These very long chain fatty acids are synthesized on the cytoplasmic leaflet of the inner membrane (IM) prior to conjugation to the disaccharide, trehalose, and transport to the periplasm.					
34944401	8	68	theme	global	1330:1335	arg1	changes					1337:1343	strikingly similar global changes	1311:1343	strikingly similar global changes in overall membrane lipid composition	1311:1381	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	6	69	theme	dihydroxycorynomycolate	1064:1086	arg1	synthesis					1041:1049	synthesis	1041:1049	synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid	1041:1130	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	1	70	theme	coryno	213:218	arg1	acids					228:232	(coryno)mycolic acids	212:232	(coryno)mycolic acids	212:232	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	3	71	theme	trehalose	500:508	arg1	monohydroxycorynomycolate					510:534	trehalose monohydroxycorynomycolate	500:534	trehalose monohydroxycorynomycolate (hTMCM)	500:542	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	3	71	theme	trehalose	500:508	arg1	hTMCM					537:541	hTMCM	537:541	hTMCM	537:541	Recent studies on Corynebacterium glutamicum have shown that acetylation of trehalose monohydroxycorynomycolate (hTMCM) promotes its transport across the inner membrane.					
34944401	8	72	theme	membrane	1356:1363	arg1	composition					1371:1381	overall membrane lipid composition	1348:1381	overall membrane lipid composition	1348:1381	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	6	73	theme	trehalose	1054:1062	arg1	dihydroxycorynomycolate					1064:1086	trehalose dihydroxycorynomycolate	1054:1086	trehalose dihydroxycorynomycolate (h2TDCM)	1054:1095	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	73	theme	trehalose	1054:1062	arg1	glycolipid					1121:1130	a major outer membrane glycolipid	1098:1130	a major outer membrane glycolipid	1098:1130	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	73	theme	trehalose	1054:1062	arg1	h2TDCM					1089:1094	h2TDCM	1089:1094	h2TDCM	1089:1094	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	6	74	dep	C.	851:852	arg1	glutamicum					854:863	glutamicum	854:863	glutamicum	854:863	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	8	75	theme	lipidomic	1245:1253	arg1	analysis					1255:1262	Comprehensive lipidomic analysis	1231:1262	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants	1231:1300	Comprehensive lipidomic analysis of the ΔtmaT, ΔmtrP and ΔmmpA mutants revealed strikingly similar global changes in overall membrane lipid composition.					
34944401	0	76	theme	Surface	58:64	arg1	Transport					66:74	Efficient Surface Transport	48:74	Efficient Surface Transport of Trehalose Lipids in Corynebacterineae	48:115	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	9	77	theme	membrane	1430:1437	arg1	transport					1439:1447	membrane transport	1430:1447	membrane transport	1430:1447	Our findings suggest that the acetylation and membrane transport of hTMCM is regulated by multiple proteins: MmpA, MtrP and TmaT, and that defects in this process lead to global, potentially compensatory changes in the composition of inner and outer membranes.					
34944401	6	78	theme	hTMCM	1001:1005	arg1	accumulation					985:996	an accumulation	982:996	an accumulation of hTMCM in the inner membrane	982:1027	Deletion of the C. glutamicum gene NCgl2761 (Rv0226c in Mycobacterium tuberculosis) abolished synthesis of acetylated hTMCM (AcTMCM), resulting in an accumulation of hTMCM in the inner membrane and reduced synthesis of trehalose dihydroxycorynomycolate (h2TDCM), a major outer membrane glycolipid.					
34944401	1	79	theme	bacteria	132:139	arg1	walls					123:127	Cell walls	118:127	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium	118:187	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	1	80	theme	mycolic	220:226	arg1	acids					228:232	(coryno)mycolic acids	212:232	(coryno)mycolic acids	212:232	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
34944401	0	81	theme	Efficient	48:56	arg1	Transport					66:74	Efficient Surface Transport	48:74	Efficient Surface Transport of Trehalose Lipids in Corynebacterineae	48:115	MmpA, a Conserved Membrane Protein Required for Efficient Surface Transport of Trehalose Lipids in Corynebacterineae.					
34944401	5	82	dep	acetylation	790:800	arg1	the					786:788	the	786:788	the	786:788	Here, we identify a third protein that is required for the acetylation and membrane transport of hTMCM.					
34944401	1	83	theme	acids	228:232	arg1	levels					202:207	high levels	197:207	high levels of (coryno)mycolic acids	197:232	Cell walls of bacteria of the genera Mycobacterium and Corynebacterium contain high levels of (coryno)mycolic acids.					
32980541	7	0	theme	relative	1158:1165	arg1	changes					1167:1173	relative changes	1158:1173	relative changes	1158:1173	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	7	0	theme	relative	1158:1165	arg1	responses					970:978	Loading responses	962:978	Loading responses	962:978	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	4	1	theme	human	526:530	arg1	samples					554:560	30 human femoral osteochondral samples	523:560	30 human femoral osteochondral samples	523:560	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	11	2	from	interrelatedness	1797:1812	arg1	cartilage					1853:1861	cartilage	1853:1861	cartilage	1853:1861	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	10	3	from	changes	1623:1629	arg1	*					1636:1636	T2*	1634:1636	T2*	1634:1636	Loading-induced decreases in T2 were significant after LC exposure (p = 0.006), while changes in T2* were ambiguous.					
32980541	8	4	theme	Dose-dependant	1253:1266	arg1	disintegration					1276:1289	Dose-dependant surface disintegration	1253:1289	Dose-dependant surface disintegration	1253:1289	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	0	5	theme	MRI	84:86	arg1	mapping					88:94	quantitative MRI mapping	71:94	quantitative MRI mapping	71:94	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	6	6	theme	Untreated	915:923	arg1	samples					925:931	Untreated samples	915:931	Untreated samples	915:931	Untreated samples served as controls (n = 10).					
32980541	6	6	theme	Untreated	915:923	arg1	controls					943:950	controls	943:950	controls (n = 10)	943:959	Untreated samples served as controls (n = 10).					
32980541	1	7	theme	tissue	171:176	arg1	structure					178:186	tissue structure	171:186	tissue structure	171:186	Cartilage functionality is determined by tissue structure and composition.					
32980541	8	8	theme	post-exposure	1355:1367	arg1	patterns					1389:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	3	9	theme	depletion	420:428	arg1	effects					348:354	the dose-dependant effects	329:354	the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality	329:455	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	4	10	theme	osteochondral	540:552	arg1	samples					554:560	30 human femoral osteochondral samples	523:560	30 human femoral osteochondral samples	523:560	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	5	11	theme	collagenase	902:912	arg1	concentration					861:873	high concentration	856:873	high concentration (HC, 1.5 mg/mL; n = 10) of collagenase	856:912	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	5	12	dep	concentration	861:873	arg1	1.5 mg/mL					880:888	1.5 mg/mL	880:888	1.5 mg/mL	880:888	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	5	12	dep	concentration	861:873	arg1	HC					876:877	HC	876:877	HC	876:877	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	11	13	theme	functionality	1836:1848	arg1	interrelatedness					1797:1812	the close interrelatedness	1787:1812	the close interrelatedness of matrix changes and functionality in cartilage	1787:1861	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	9	14	dep	increased	1482:1490	arg1	p = 0.008					1525:1533	p = 0.008	1525:1533	p = 0.008	1525:1533	While T1 generally decreased with loading, regardless of collagenase exposure, T1ρ increased significantly after HC exposure (p = 0.008).					
32980541	7	15	theme	non-parametric	1219:1232	arg1	tests					1246:1250	adequate parametric and non-parametric statistical tests	1195:1250	adequate parametric and non-parametric statistical tests	1195:1250	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	0	16	theme	exposure	114:121	arg1	model					123:127	The collagenase exposure model	98:127	The collagenase exposure model	98:127	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	7	17	theme	adequate	1195:1202	arg1	tests					1246:1250	adequate parametric and non-parametric statistical tests	1195:1250	adequate parametric and non-parametric statistical tests	1195:1250	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	5	18	dep	HC	876:877	arg1	n = 10					891:896	n = 10	891:896	n = 10	891:896	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	3	19	theme	T2	488:489	arg1	*					498:498	serial T1, T1ρ, T2, and T2*	472:498	serial T1, T1ρ, T2, and T2* mapping under loading	472:520	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	0	20	theme	collagenase	102:112	arg1	model					123:127	The collagenase exposure model	98:127	The collagenase exposure model	98:127	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	0	21	theme	cartilage	38:46	arg1	functionality					48:60	cartilage functionality	38:60	cartilage functionality	38:60	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	11	22	theme	matrix	1744:1749	arg1	changes					1751:1757	moderate and severe matrix changes	1724:1757	moderate and severe matrix changes	1724:1757	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	11	23	from	changes	1694:1700	arg1	T1ρ					1712:1714	T1ρ	1712:1714	T1ρ	1712:1714	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	11	23	from	changes	1694:1700	arg1	T2					1705:1706	T2	1705:1706	T2	1705:1706	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	7	24	dep	sub-pistonal	1048:1059	arg1	i.e.					1043:1046	i.e.	1043:1046	i.e.	1043:1046	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	3	25	theme	collagenase-induced	359:377	arg1	disintegration					388:401	collagenase-induced collagen disintegration	359:401	collagenase-induced collagen disintegration	359:401	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	0	26	theme	quantitative	71:82	arg1	mapping					88:94	quantitative MRI mapping	71:94	quantitative MRI mapping	71:94	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	3	27	theme	serial	472:477	arg1	T1					479:480	serial T1	472:480	serial T1	472:480	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	3	28	theme	T1ρ	483:485	arg1	*					498:498	serial T1, T1ρ, T2, and T2*	472:498	serial T1, T1ρ, T2, and T2* mapping under loading	472:520	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	7	29	theme	loaded	1035:1040	arg1	regions					1108:1114	the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions	1022:1114	the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions	1022:1114	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	8	30	theme	pre-	1346:1349	arg1	patterns					1389:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	8	31	theme	different	1336:1344	arg1	patterns					1389:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	9	32	theme	collagenase	1456:1466	arg1	exposure					1468:1475	collagenase exposure	1456:1475	collagenase exposure	1456:1475	While T1 generally decreased with loading, regardless of collagenase exposure, T1ρ increased significantly after HC exposure (p = 0.008).					
32980541	3	33	theme	cartilage	433:441	arg1	functionality					443:455	cartilage functionality	433:455	cartilage functionality	433:455	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	4	34	theme	clinical	585:592	arg1	Achieva					613:619	Achieva	613:619	Achieva	613:619	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	34	theme	clinical	585:592	arg1	scanner					604:610	a clinical 3.0 T MRI scanner	583:610	a clinical 3.0 T MRI scanner (Achieva, Philips)	583:629	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	3	35	theme	early	298:302	arg1	osteoarthritis					304:317	early osteoarthritis	298:317	early osteoarthritis	298:317	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	8	36	theme	response-to-loading	1369:1387	arg1	patterns					1389:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	distinctly different pre- and post-exposure response-to-loading patterns	1325:1396	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	11	37	theme	changes	1824:1830	arg1	interrelatedness					1797:1812	the close interrelatedness	1787:1812	the close interrelatedness of matrix changes and functionality in cartilage	1787:1861	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	4	38	theme	reference	647:655	arg1	configuration					657:669	the unloaded reference configuration	634:669	the unloaded reference configuration (δ0)	634:674	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	38	theme	reference	647:655	arg1	δ0					672:673	δ0	672:673	δ0	672:673	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	0	39	theme	imaging	16:22	arg1	correlates					24:33	the imaging correlates	12:33	the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model	12:127	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	4	40	theme	MRI	600:602	arg1	Achieva					613:619	Achieva	613:619	Achieva	613:619	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	40	theme	MRI	600:602	arg1	scanner					604:610	a clinical 3.0 T MRI scanner	583:610	a clinical 3.0 T MRI scanner (Achieva, Philips)	583:629	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	3	41	theme	osteoarthritis	304:317	arg1	study					289:293	This pathomimetical study	269:293	This pathomimetical study of early osteoarthritis	269:317	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	10	42	from	decreases	1553:1561	arg1	T2					1566:1567	T2	1566:1567	T2	1566:1567	Loading-induced decreases in T2 were significant after LC exposure (p = 0.006), while changes in T2* were ambiguous.					
32980541	4	43	theme	indentation	719:729	arg1	loading					731:737	pressure-controlled quasi-static indentation loading	686:737	pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2)	686:771	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	44	theme	unloaded	638:645	arg1	configuration					657:669	the unloaded reference configuration	634:669	the unloaded reference configuration (δ0)	634:674	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	44	theme	unloaded	638:645	arg1	δ0					672:673	δ0	672:673	δ0	672:673	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	3	45	theme	T1	479:480	arg1	*					498:498	serial T1, T1ρ, T2, and T2*	472:498	serial T1, T1ρ, T2, and T2* mapping under loading	472:520	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	4	46	theme	T	598:598	arg1	Achieva					613:619	Achieva	613:619	Achieva	613:619	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	46	theme	T	598:598	arg1	scanner					604:610	a clinical 3.0 T MRI scanner	583:610	a clinical 3.0 T MRI scanner (Achieva, Philips)	583:629	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	7	47	theme	entire	1004:1009	arg1	sample					1011:1016	the entire sample	1000:1016	the entire sample	1000:1016	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	4	48	dep	Achieva	613:619	arg1	Philips					622:628	Philips	622:628	Philips	622:628	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	2	49	theme	premature	245:253	arg1	degeneration					255:266	premature degeneration	245:266	premature degeneration	245:266	If altered, cartilage is predisposed to premature degeneration.					
32980541	10	50	theme	T2	1634:1635	arg1	*					1636:1636	T2*	1634:1636	T2*	1634:1636	Loading-induced decreases in T2 were significant after LC exposure (p = 0.006), while changes in T2* were ambiguous.					
32980541	7	51	theme	adjacent	1078:1085	arg1	regions					1108:1114	the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions	1022:1114	the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions	1022:1114	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	7	52	dep	loaded	1035:1040	arg1	peri‑pistonal					1093:1105	peri‑pistonal	1093:1105	peri‑pistonal	1093:1105	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	7	52	dep	loaded	1035:1040	arg1	sub-pistonal					1048:1059	sub-pistonal	1048:1059	sub-pistonal	1048:1059	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	11	53	theme	matrix	1817:1822	arg1	changes					1824:1830	matrix changes	1817:1830	matrix changes	1817:1830	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	11	54	theme	aberrant	1669:1676	arg1	changes					1694:1700	aberrant loading-induced changes	1669:1700	aberrant loading-induced changes in T2 and T1ρ	1669:1714	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	11	55	theme	moderate	1724:1731	arg1	changes					1751:1757	moderate and severe matrix changes	1724:1757	moderate and severe matrix changes	1724:1757	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	7	56	theme	Loading	962:968	arg1	changes					1167:1173	relative changes	1158:1173	relative changes	1158:1173	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	7	56	theme	Loading	962:968	arg1	responses					970:978	Loading responses	962:978	Loading responses	962:978	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	3	57	theme	T2	496:497	arg1	*					498:498	serial T1, T1ρ, T2, and T2*	472:498	serial T1, T1ρ, T2, and T2* mapping under loading	472:520	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	4	58	from	imaging	572:578	arg1	Achieva					613:619	Achieva	613:619	Achieva	613:619	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	58	from	imaging	572:578	arg1	scanner					604:610	a clinical 3.0 T MRI scanner	583:610	a clinical 3.0 T MRI scanner (Achieva, Philips)	583:629	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	10	59	theme	LC	1592:1593	arg1	p = 0.006					1605:1613	p = 0.006	1605:1613	p = 0.006	1605:1613	Loading-induced decreases in T2 were significant after LC exposure (p = 0.006), while changes in T2* were ambiguous.					
32980541	10	59	theme	LC	1592:1593	arg1	exposure					1595:1602	LC exposure	1592:1602	LC exposure (p = 0.006)	1592:1614	Loading-induced decreases in T2 were significant after LC exposure (p = 0.006), while changes in T2* were ambiguous.					
32980541	3	60	theme	dose-dependant	333:346	arg1	effects					348:354	the dose-dependant effects	329:354	the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality	329:455	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	11	61	theme	close	1791:1795	arg1	interrelatedness					1797:1812	the close interrelatedness	1787:1812	the close interrelatedness of matrix changes and functionality in cartilage	1787:1861	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	7	62	dep	peri‑pistonal	1093:1105	arg1	i.e.					1088:1091	i.e. peri‑pistonal	1088:1105	i.e. peri‑pistonal	1088:1105	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	7	63	theme	statistical	1234:1244	arg1	tests					1246:1250	adequate parametric and non-parametric statistical tests	1195:1250	adequate parametric and non-parametric statistical tests	1195:1250	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	0	64	theme	functionality	48:60	arg1	correlates					24:33	the imaging correlates	12:33	the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model	12:127	Identifying the imaging correlates of cartilage functionality based on quantitative MRI mapping - The collagenase exposure model.					
32980541	3	65	theme	proteoglycan	407:418	arg1	depletion					420:428	proteoglycan depletion	407:428	proteoglycan depletion	407:428	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	3	66	theme	pathomimetical	274:287	arg1	study					289:293	This pathomimetical study	269:293	This pathomimetical study of early osteoarthritis	269:317	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	7	67	theme	parametric	1204:1213	arg1	tests					1246:1250	adequate parametric and non-parametric statistical tests	1195:1250	adequate parametric and non-parametric statistical tests	1195:1250	Loading responses were determined for the entire sample and the directly loaded (i.e. sub-pistonal) and bilaterally adjacent (i.e. peri‑pistonal) regions, referenced histologically, quantified as relative changes, and analysed using adequate parametric and non-parametric statistical tests.					
32980541	5	68	dep	LC	830:831	arg1	n = 10					845:850	n = 10	845:850	n = 10	845:850	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	1	69	theme	Cartilage	130:138	arg1	functionality					140:152	Cartilage functionality	130:152	Cartilage functionality	130:152	Cartilage functionality is determined by tissue structure and composition.					
32980541	2	70	dep	altered	208:214	arg1	If					205:206	If	205:206	If	205:206	If altered, cartilage is predisposed to premature degeneration.					
32980541	8	71	theme	tissue	1295:1300	arg1	loss					1302:1305	tissue loss	1295:1305	tissue loss	1295:1305	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	5	72	theme	high	856:859	arg1	concentration					861:873	high concentration	856:873	high concentration (HC, 1.5 mg/mL; n = 10) of collagenase	856:912	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	9	73	theme	HC	1512:1513	arg1	exposure					1515:1522	HC exposure	1512:1522	HC exposure	1512:1522	While T1 generally decreased with loading, regardless of collagenase exposure, T1ρ increased significantly after HC exposure (p = 0.008).					
32980541	3	74	theme	collagen	379:386	arg1	disintegration					388:401	collagenase-induced collagen disintegration	359:401	collagenase-induced collagen disintegration	359:401	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	11	75	theme	severe	1737:1742	arg1	changes					1751:1757	moderate and severe matrix changes	1724:1757	moderate and severe matrix changes	1724:1757	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	5	76	dep	low	825:827	arg1	LC					830:831	LC	830:831	LC	830:831	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	5	76	dep	low	825:827	arg1	0.5 mg/mL					834:842	0.5 mg/mL	834:842	0.5 mg/mL	834:842	Imaging was performed before and after exposure to low (LC, 0.5 mg/mL; n = 10) or high concentration (HC, 1.5 mg/mL; n = 10) of collagenase.					
32980541	4	77	theme	quasi-static	706:717	arg1	loading					731:737	pressure-controlled quasi-static indentation loading	686:737	pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2)	686:771	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	8	78	theme	surface	1268:1274	arg1	disintegration					1276:1289	Dose-dependant surface disintegration	1253:1289	Dose-dependant surface disintegration	1253:1289	Dose-dependant surface disintegration and tissue loss were reflected by distinctly different pre- and post-exposure response-to-loading patterns.					
32980541	10	79	theme	Loading-induced	1537:1551	arg1	decreases					1553:1561	Loading-induced decreases	1537:1561	Loading-induced decreases in T2	1537:1567	Loading-induced decreases in T2 were significant after LC exposure (p = 0.006), while changes in T2* were ambiguous.					
32980541	3	80	theme	disintegration	388:401	arg1	effects					348:354	the dose-dependant effects	329:354	the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality	329:455	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
32980541	4	81	theme	femoral	532:538	arg1	samples					554:560	30 human femoral osteochondral samples	523:560	30 human femoral osteochondral samples	523:560	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	4	82	theme	pressure-controlled	686:704	arg1	loading					731:737	pressure-controlled quasi-static indentation loading	686:737	pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2)	686:771	30 human femoral osteochondral samples underwent imaging on a clinical 3.0 T MRI scanner (Achieva, Philips) in the unloaded reference configuration (δ0) and under pressure-controlled quasi-static indentation loading to 15.1 N (δ1) and to 28.6 N (δ2).					
32980541	11	83	theme	loading-induced	1678:1692	arg1	changes					1694:1700	aberrant loading-induced changes	1669:1700	aberrant loading-induced changes in T2 and T1ρ	1669:1714	In conclusion, aberrant loading-induced changes in T2 and T1ρ reflect moderate and severe matrix changes, respectively, and indicate the close interrelatedness of matrix changes and functionality in cartilage.					
32980541	3	84	from	effects	348:354	arg1	functionality					443:455	cartilage functionality	433:455	cartilage functionality	433:455	This pathomimetical study of early osteoarthritis evaluated the dose-dependant effects of collagenase-induced collagen disintegration and proteoglycan depletion on cartilage functionality as assessed by serial T1, T1ρ, T2, and T2* mapping under loading.					
34180787	8	0	theme	consumed	1153:1160	arg1	quantities					1167:1176	consumed food quantities	1153:1176	consumed food quantities from the 3046 single households	1153:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	8	1	from	households	1199:1208	arg1	quantities					1167:1176	consumed food quantities	1153:1176	consumed food quantities from the 3046 single households	1153:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	10	2	theme	due	1383:1385	arg1	adjustments					1371:1381	the greatest adjustments	1358:1381	the greatest adjustments due to lactose reduction	1358:1406	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	2	3	theme	alternative	355:365	arg1	form					372:375	alternative diet form	355:375	alternative diet form rich in Ca	355:386	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	1	4	theme	main	275:278	arg1	source					280:285	a main source	273:285	a main source of Ca.	273:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	1	4	theme	main	275:278	arg1	products					254:261	lactose-containing dairy products	229:261	lactose-containing dairy products which are a main source of Ca.	229:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	10	5	theme	greatest	1362:1369	arg1	adjustments					1371:1381	the greatest adjustments	1358:1381	the greatest adjustments due to lactose reduction	1358:1406	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	2	6	from	interest	340:347	arg1	particular					297:306	particular	297:306	particular	297:306	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	4	7	theme	different	668:676	arg1	contents					686:693	different lactose contents	668:693	different lactose contents	668:693	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	0	8	with	people	119:124	arg1	intolerance					139:149	lactose intolerance	131:149	lactose intolerance	131:149	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	3	9	theme	different	522:530	arg1	options					532:538	different options	522:538	different options	522:538	This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.					
34180787	5	10	theme	food	855:858	arg1	baskets					860:866	the food baskets	851:866	the food baskets	851:866	By comparing the costs and the composition of the food baskets, recommendations for a lactose-reduced diet were derived.					
34180787	8	11	theme	single	1192:1197	arg1	households					1199:1208	the 3046 single households	1183:1208	the 3046 single households	1183:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	9	12	theme	minimum	1223:1229	arg1	costs					1247:1251	The minimum additional food costs	1219:1251	The minimum additional food costs	1219:1251	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	11	13	theme	normal	1502:1507	arg1	milk					1509:1512	normal milk	1502:1512	normal milk	1502:1512	In this group, with a rising lactose limit, normal milk was increasingly replaced by lactose-free milk.					
34180787	9	14	theme	food	1242:1245	arg1	costs					1247:1251	The minimum additional food costs	1219:1251	The minimum additional food costs	1219:1251	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	1	15	theme	lactose-containing	229:246	arg1	source					280:285	a main source	273:285	a main source of Ca.	273:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	1	15	theme	lactose-containing	229:246	arg1	products					254:261	lactose-containing dairy products	229:261	lactose-containing dairy products which are a main source of Ca.	229:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	13	16	theme	lactose-free	1716:1727	arg1	milk					1729:1732	lactose-free milk	1716:1732	lactose-free milk	1716:1732	When suffering from lactose intolerance, switching to lactose-free milk seems to be the most cost-effective way to cover nutrient requirements.					
34180787	4	17	theme	basic	734:738	arg1	model					740:744	a basic model	732:744	a basic model	732:744	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	4	18	dep	DESIGN	617:622	arg1	programming					637:647	linear programming	630:647	linear programming	630:647	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	4	18	dep	DESIGN	617:622	arg1	Using					624:628	Using	624:628	Using	624:628	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	9	19	theme	intolerance	1287:1297	arg1	severity					1267:1274	the severity	1263:1274	the severity of lactose intolerance and range from 0·2 % to 6·1 % per month	1263:1337	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	11	20	theme	lactose	1487:1493	arg1	limit					1495:1499	a rising lactose limit	1478:1499	a rising lactose limit	1478:1499	In this group, with a rising lactose limit, normal milk was increasingly replaced by lactose-free milk.					
34180787	9	21	theme	range	1303:1307	arg1	severity					1267:1274	the severity	1263:1274	the severity of lactose intolerance and range from 0·2 % to 6·1 % per month	1263:1337	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	7	22	theme	panel	967:971	arg1	representative					981:994	PARTICIPANTS A consumer panel dataset representative	943:994	PARTICIPANTS A consumer panel dataset representative for Germany	943:1006	PARTICIPANTS A consumer panel dataset representative for Germany is used for the calculations.					
34180787	1	23	theme	lactose	174:180	arg1	intolerance					182:192	lactose intolerance	174:192	lactose intolerance	174:192	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	9	24	dep	RESULTS	1211:1217	arg1	depend					1253:1258	depend	1253:1258	depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month	1253:1337	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	8	25	from	children	1124:1131	arg1	quantities					1167:1176	consumed food quantities	1153:1176	consumed food quantities from the 3046 single households	1153:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	13	26	theme	lactose	1682:1688	arg1	intolerance					1690:1700	lactose intolerance	1682:1700	lactose intolerance	1682:1700	When suffering from lactose intolerance, switching to lactose-free milk seems to be the most cost-effective way to cover nutrient requirements.					
34180787	0	27	theme	cost-effective	88:101	arg1	alternative					103:113	a cost-effective alternative	86:113	a cost-effective alternative for people with lactose intolerance	86:149	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	0	27	theme	cost-effective	88:101	arg1	costs					11:15	Additional costs	0:15	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes	0:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	1	28	with	People	162:167	arg1	intolerance					182:192	lactose intolerance	174:192	lactose intolerance	174:192	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	3	29	theme	additional	459:468	arg1	costs					470:474	the additional costs	455:474	the additional costs of lactose-reduced diets	455:499	This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.					
34180787	12	30	theme	higher	1643:1648	arg1	costs					1655:1659	higher food costs	1643:1659	higher food costs	1643:1659	CONCLUSION It was shown that a lactose-reduced diet is generally associated with higher food costs.					
34180787	10	31	theme	product	1443:1449	arg1	group					1451:1455	the dairy product group	1433:1455	the dairy product group	1433:1455	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	0	32	theme	Additional	0:9	arg1	alternative					103:113	a cost-effective alternative	86:113	a cost-effective alternative for people with lactose intolerance	86:149	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	0	32	theme	Additional	0:9	arg1	costs					11:15	Additional costs	0:15	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes	0:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	1	33	theme	Ca.	290:292	arg1	source					280:285	a main source	273:285	a main source of Ca.	273:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	1	33	theme	Ca.	290:292	arg1	products					254:261	lactose-containing dairy products	229:261	lactose-containing dairy products which are a main source of Ca.	229:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	12	34	theme	lactose-reduced	1593:1607	arg1	diet					1609:1612	a lactose-reduced diet	1591:1612	a lactose-reduced diet	1591:1612	CONCLUSION It was shown that a lactose-reduced diet is generally associated with higher food costs.					
34180787	0	35	dep	product	62:68	arg1	substitutes					70:80	substitutes	70:80	substitutes	70:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	2	36	theme	lowest	401:406	arg1	costs					419:423	the lowest additional costs	397:423	the lowest additional costs	397:423	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	0	37	theme	diets	36:40	arg1	alternative					103:113	a cost-effective alternative	86:113	a cost-effective alternative for people with lactose intolerance	86:149	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	0	37	theme	diets	36:40	arg1	costs					11:15	Additional costs	0:15	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes	0:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	8	38	from	information	1138:1148	arg1	quantities					1167:1176	consumed food quantities	1153:1176	consumed food quantities from the 3046 single households	1153:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	2	39	from	Ca	385:386	arg1	rich					377:380	rich	377:380	rich	377:380	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	8	40	attach	derived	1077:1083	arg1	households					1105:1114	the 9429 adult households	1090:1114	the 9429 adult households without children, and information on consumed food quantities from the 3046 single households	1090:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	8	40	attach	derived	1077:1083	arg2	Information					1038:1048	Information	1038:1048	Information on prices and nutrients	1038:1072	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	3	41	theme	lactose-reduced	479:493	arg1	diets					495:499	lactose-reduced diets	479:499	lactose-reduced diets	479:499	This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.					
34180787	0	42	theme	lactose-free	43:54	arg1	product					62:68	lactose-free dairy product	43:68	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes	0:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	1	43	theme	dairy	248:252	arg1	source					280:285	a main source	273:285	a main source of Ca.	273:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	1	43	theme	dairy	248:252	arg1	products					254:261	lactose-containing dairy products	229:261	lactose-containing dairy products which are a main source of Ca.	229:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	2	44	theme	rich	377:380	arg1	form					372:375	alternative diet form	355:375	alternative diet form rich in Ca	355:386	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	13	45	theme	nutrient	1783:1790	arg1	requirements					1792:1803	nutrient requirements	1783:1803	nutrient requirements	1783:1803	When suffering from lactose intolerance, switching to lactose-free milk seems to be the most cost-effective way to cover nutrient requirements.					
34180787	2	46	theme	diet	367:370	arg1	form					372:375	alternative diet form	355:375	alternative diet form rich in Ca	355:386	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	8	47	theme	food	1162:1165	arg1	quantities					1167:1176	consumed food quantities	1153:1176	consumed food quantities from the 3046 single households	1153:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	13	48	theme	cost-effective	1755:1768	arg1	way					1770:1772	the most cost-effective way	1746:1772	the most cost-effective way to cover nutrient requirements	1746:1803	When suffering from lactose intolerance, switching to lactose-free milk seems to be the most cost-effective way to cover nutrient requirements.					
34180787	13	48	theme	cost-effective	1755:1768	arg1	switching					1703:1711	switching	1703:1711	switching to lactose-free milk	1703:1732	When suffering from lactose intolerance, switching to lactose-free milk seems to be the most cost-effective way to cover nutrient requirements.					
34180787	3	49	theme	cost-effective	559:572	arg1	alternative					574:584	the most cost-effective alternative	550:584	the most cost-effective alternative within a lactose-reduced diet	550:614	This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.					
34180787	5	50	theme	baskets	860:866	arg1	composition					836:846	the composition	832:846	the composition of the food baskets	832:866	By comparing the costs and the composition of the food baskets, recommendations for a lactose-reduced diet were derived.					
34180787	5	50	theme	baskets	860:866	arg1	costs					822:826	the costs	818:826	the costs	818:826	By comparing the costs and the composition of the food baskets, recommendations for a lactose-reduced diet were derived.					
34180787	2	51	from	rich	377:380	arg1	Ca					385:386	Ca	385:386	Ca	385:386	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	4	52	theme	lactose	678:684	arg1	contents					686:693	different lactose contents	668:693	different lactose contents	668:693	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	10	53	theme	lactose	1390:1396	arg1	reduction					1398:1406	lactose reduction	1390:1406	lactose reduction	1390:1406	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	0	54	theme	lactose	131:137	arg1	intolerance					139:149	lactose intolerance	131:149	lactose intolerance	131:149	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	9	55	theme	additional	1231:1240	arg1	costs					1247:1251	The minimum additional food costs	1219:1251	The minimum additional food costs	1219:1251	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	2	56	theme	low-income	313:322	arg1	people					324:329	low-income people	313:329	low-income people	313:329	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	8	57	from	Information	1038:1048	arg1	nutrients					1064:1072	nutrients	1064:1072	nutrients	1064:1072	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	8	57	from	Information	1038:1048	arg1	prices					1053:1058	prices	1053:1058	prices	1053:1058	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	6	58	dep	Germany	934:940	arg1	SETTING					926:932	SETTING	926:932	SETTING	926:932	SETTING Germany.					
34180787	5	59	theme	lactose-reduced	891:905	arg1	diet					907:910	a lactose-reduced diet	889:910	a lactose-reduced diet	889:910	By comparing the costs and the composition of the food baskets, recommendations for a lactose-reduced diet were derived.					
34180787	3	60	theme	lactose-reduced	595:609	arg1	diet					611:614	a lactose-reduced diet	593:614	a lactose-reduced diet	593:614	This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.					
34180787	10	61	located	observed	1417:1424	arg1	group					1451:1455	the dairy product group	1433:1455	the dairy product group	1433:1455	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	10	61	located	observed	1417:1424	arg2	adjustments					1371:1381	the greatest adjustments	1358:1381	the greatest adjustments due to lactose reduction	1358:1406	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	7	62	used	used	1011:1014	arg2	representative					981:994	PARTICIPANTS A consumer panel dataset representative	943:994	PARTICIPANTS A consumer panel dataset representative for Germany	943:1006	PARTICIPANTS A consumer panel dataset representative for Germany is used for the calculations.					
34180787	9	63	theme	lactose	1279:1285	arg1	intolerance					1287:1297	lactose intolerance	1279:1297	lactose intolerance	1279:1297	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	1	64	theme	OBJECTIVE	152:160	arg1	People					162:167	OBJECTIVE People	152:167	OBJECTIVE People with lactose intolerance	152:192	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	11	65	theme	rising	1480:1485	arg1	limit					1495:1499	a rising lactose limit	1478:1499	a rising lactose limit	1478:1499	In this group, with a rising lactose limit, normal milk was increasingly replaced by lactose-free milk.					
34180787	12	66	theme	food	1650:1653	arg1	costs					1655:1659	higher food costs	1643:1659	higher food costs	1643:1659	CONCLUSION It was shown that a lactose-reduced diet is generally associated with higher food costs.					
34180787	9	67	dep	%	1327:1327	arg1	to					1320:1321	to	1320:1321	to	1320:1321	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	7	68	theme	dataset	973:979	arg1	representative					981:994	PARTICIPANTS A consumer panel dataset representative	943:994	PARTICIPANTS A consumer panel dataset representative for Germany	943:1006	PARTICIPANTS A consumer panel dataset representative for Germany is used for the calculations.					
34180787	4	69	theme	normal	760:765	arg1	diet					767:770	a normal diet	758:770	a normal diet without a limitation of lactose	758:802	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	9	70	theme	%	1318:1318	arg1	%					1327:1327	0·2 % to 6·1 %	1314:1327	0·2 % to 6·1 % per month	1314:1337	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	7	71	theme	consumer	958:965	arg1	panel					967:971	A consumer panel	956:971	PARTICIPANTS A consumer panel dataset representative for Germany	943:1006	PARTICIPANTS A consumer panel dataset representative for Germany is used for the calculations.					
34180787	9	72	from	%	1327:1327	arg1	severity					1267:1274	the severity	1263:1274	the severity of lactose intolerance and range from 0·2 % to 6·1 % per month	1263:1337	RESULTS The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month.					
34180787	7	73	theme	PARTICIPANTS	943:954	arg1	representative					981:994	PARTICIPANTS A consumer panel dataset representative	943:994	PARTICIPANTS A consumer panel dataset representative for Germany	943:1006	PARTICIPANTS A consumer panel dataset representative for Germany is used for the calculations.					
34180787	10	74	theme	dairy	1437:1441	arg1	group					1451:1455	the dairy product group	1433:1455	the dairy product group	1433:1455	It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group.					
34180787	0	75	theme	lactose-reduced	20:34	arg1	diets					36:40	lactose-reduced diets	20:40	lactose-reduced diets	20:40	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	2	76	theme	additional	408:417	arg1	costs					419:423	the lowest additional costs	397:423	the lowest additional costs	397:423	In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs.					
34180787	8	77	theme	adult	1099:1103	arg1	households					1105:1114	the 9429 adult households	1090:1114	the 9429 adult households without children, and information on consumed food quantities from the 3046 single households	1090:1208	Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.					
34180787	0	78	theme	dairy	56:60	arg1	product					62:68	lactose-free dairy product	43:68	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes	0:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	4	79	with	baskets	655:661	arg1	contents					686:693	different lactose contents	668:693	different lactose contents	668:693	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	11	80	theme	lactose-free	1543:1554	arg1	milk					1556:1559	lactose-free milk	1543:1559	lactose-free milk	1543:1559	In this group, with a rising lactose limit, normal milk was increasingly replaced by lactose-free milk.					
34180787	3	81	theme	diets	495:499	arg1	costs					470:474	the additional costs	455:474	the additional costs of lactose-reduced diets	455:499	This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.					
34180787	1	82	theme	products	254:261	arg1	consumption					214:224	their consumption	208:224	their consumption of lactose-containing dairy products which are a main source of Ca.	208:292	OBJECTIVE People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca.					
34180787	4	83	theme	food	650:653	arg1	baskets					655:661	food baskets	650:661	food baskets with different lactose contents	650:693	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	0	84	dep	costs	11:15	arg1	product					62:68	lactose-free dairy product	43:68	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes	0:80	Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance.					
34180787	4	85	theme	linear	630:635	arg1	programming					637:647	linear programming	630:647	linear programming	630:647	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34180787	12	86	dep	CONCLUSION	1562:1571	arg1	shown					1580:1584	shown	1580:1584	was shown that a lactose-reduced diet is generally associated with higher food costs	1576:1659	CONCLUSION It was shown that a lactose-reduced diet is generally associated with higher food costs.					
34180787	4	87	theme	lactose	796:802	arg1	limitation					782:791	a limitation	780:791	a limitation of lactose	780:802	DESIGN Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose.					
34201854	2	0	with	textiles	630:637	arg1	H2O2					644:647	H2O2	644:647	H2O2	644:647	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	3	1	theme	emission	1004:1011	arg1	MP-AES					1027:1032	MP-AES	1027:1032	MP-AES	1027:1032	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	1	theme	emission	1004:1011	arg1	spectroscopy					1013:1024	microwave plasma atomic emission spectroscopy	980:1024	microwave plasma atomic emission spectroscopy (MP-AES)	980:1033	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	4	2	theme	mg	1103:1104	arg1	incorporation					1068:1080	a maximum incorporation	1058:1080	a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile	1058:1131	The composites showed a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile.					
34201854	3	3	theme	photoelectron	766:778	arg1	XPS					794:796	XPS	794:796	XPS	794:796	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	3	theme	photoelectron	766:778	arg1	spectroscopy					780:791	X-ray photoelectron spectroscopy	760:791	X-ray photoelectron spectroscopy (XPS)	760:797	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	4	theme	cotton-chitosan	935:949	arg1	composites					951:960	cotton-chitosan composites	935:960	cotton-chitosan composites	935:960	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	1	5	theme	due	155:157	arg1	problem					147:153	a serious environmental problem	123:153	a serious environmental problem due to their accumulation and toxicity	123:192	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	5	theme	due	155:157	arg1	metals					103:108	Heavy metals	97:108	Heavy metals in water	97:117	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	5	6	theme	Young	1188:1192	arg1	modulus					1196:1202	a Young's modulus	1186:1202	a Young's modulus approximately 1 MPa higher than that of cotton textile	1186:1257	A tensile strength analysis of the composite showed a Young's modulus approximately 1 MPa higher than that of cotton textile.					
34201854	6	7	theme	ions	1283:1286	arg1	adsorption					1264:1273	The adsorption	1260:1273	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C	1260:1343	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	6	7	theme	ions	1283:1286	arg1	%					1357:1357	circa 74%	1349:1357	circa 74%	1349:1357	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	3	8	theme	plasma	990:995	arg1	MP-AES					1027:1032	MP-AES	1027:1032	MP-AES	1027:1032	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	8	theme	plasma	990:995	arg1	spectroscopy					1013:1024	microwave plasma atomic emission spectroscopy	980:1024	microwave plasma atomic emission spectroscopy (MP-AES)	980:1033	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	2	9	theme	cotton	623:628	arg1	textiles					630:637	oxidized cotton textiles	614:637	oxidized cotton textiles with H2O2	614:647	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	5	10	theme	tensile	1136:1142	arg1	analysis					1153:1160	A tensile strength analysis	1134:1160	A tensile strength analysis of the composite	1134:1177	A tensile strength analysis of the composite showed a Young's modulus approximately 1 MPa higher than that of cotton textile.					
34201854	2	11	theme	nontoxic	482:489	arg1	acids					519:523	carboxylic acids	508:523	carboxylic acids as crosslinkers and NaH2PO4	508:551	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	2	11	theme	nontoxic	482:489	arg1	reagents					491:498	nontoxic reagents	482:498	nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4	482:551	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	3	12	theme	atomic	997:1002	arg1	MP-AES					1027:1032	MP-AES	1027:1032	MP-AES	1027:1032	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	12	theme	atomic	997:1002	arg1	spectroscopy					1013:1024	microwave plasma atomic emission spectroscopy	980:1024	microwave plasma atomic emission spectroscopy (MP-AES)	980:1033	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	2	13	theme	oxidized	614:621	arg1	textiles					630:637	oxidized cotton textiles	614:637	oxidized cotton textiles with H2O2	614:647	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	7	14	theme	"	1504:1504	arg1	procedures					1506:1515	"green" procedures	1498:1515	"green" procedures	1498:1515	This work is an approach to demonstrate the potential of these polysaccharides, modified by "green" procedures to remove pollutants from water.					
34201854	5	15	theme	strength	1144:1151	arg1	analysis					1153:1160	A tensile strength analysis	1134:1160	A tensile strength analysis of the composite	1134:1177	A tensile strength analysis of the composite showed a Young's modulus approximately 1 MPa higher than that of cotton textile.					
34201854	4	16	theme	maximum	1060:1066	arg1	incorporation					1068:1080	a maximum incorporation	1058:1080	a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile	1058:1131	The composites showed a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile.					
34201854	6	17	theme	aqueous	1310:1316	arg1	solution					1318:1325	an aqueous solution	1307:1325	an aqueous solution	1307:1325	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	3	18	theme	fourier-transform	687:703	arg1	FTIR					728:731	FTIR	728:731	FTIR	728:731	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	18	theme	fourier-transform	687:703	arg1	spectroscopy					714:725	fourier-transform infrared spectroscopy	687:725	fourier-transform infrared spectroscopy (FTIR)	687:732	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	2	19	theme	covalent	587:594	arg1	crosslinkage					596:607	chitosan covalent crosslinkage	578:607	chitosan covalent crosslinkage into oxidized cotton textiles with H2O2	578:647	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	3	20	dep	microscopy	835:844	arg1	SEM					855:857	SEM	855:857	SEM	855:857	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	20	dep	microscopy	835:844	arg1	AFM					847:849	AFM	847:849	AFM	847:849	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	4	21	theme	chitosan	1085:1092	arg1	incorporation					1068:1080	a maximum incorporation	1058:1080	a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile	1058:1131	The composites showed a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile.					
34201854	3	22	theme	infrared	705:712	arg1	FTIR					728:731	FTIR	728:731	FTIR	728:731	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	22	theme	infrared	705:712	arg1	spectroscopy					714:725	fourier-transform infrared spectroscopy	687:725	fourier-transform infrared spectroscopy (FTIR)	687:732	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	2	23	theme	chitosan	578:585	arg1	crosslinkage					596:607	chitosan covalent crosslinkage	578:607	chitosan covalent crosslinkage into oxidized cotton textiles with H2O2	578:647	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	3	24	theme	microwave	980:988	arg1	MP-AES					1027:1032	MP-AES	1027:1032	MP-AES	1027:1032	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	24	theme	microwave	980:988	arg1	spectroscopy					1013:1024	microwave plasma atomic emission spectroscopy	980:1024	microwave plasma atomic emission spectroscopy (MP-AES)	980:1033	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	5	25	theme	higher	1224:1229	arg1	modulus					1196:1202	a Young's modulus	1186:1202	a Young's modulus approximately 1 MPa higher than that of cotton textile	1186:1257	A tensile strength analysis of the composite showed a Young's modulus approximately 1 MPa higher than that of cotton textile.					
34201854	6	26	theme	lead	1278:1281	arg1	ions					1283:1286	lead ions	1278:1286	lead ions with composites in an aqueous solution	1278:1325	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	2	27	theme	heavy	406:410	arg1	metals					412:417	heavy metals	406:417	heavy metals	406:417	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	3	28	theme	elemental	735:743	arg1	EA					755:756	EA	755:756	EA	755:756	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	28	theme	elemental	735:743	arg1	analysis					745:752	elemental analysis	735:752	elemental analysis (EA)	735:757	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	1	29	theme	Heavy	97:101	arg1	problem					147:153	a serious environmental problem	123:153	a serious environmental problem due to their accumulation and toxicity	123:192	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	29	theme	Heavy	97:101	arg1	metals					103:108	Heavy metals	97:108	Heavy metals in water	97:117	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	2	30	theme	carboxylic	508:517	arg1	acids					519:523	carboxylic acids	508:523	carboxylic acids as crosslinkers and NaH2PO4	508:551	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	3	31	theme	tensile	865:871	arg1	strength					873:880	tensile strength	865:880	tensile strength	865:880	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	5	32	theme	textile	1251:1257	arg1	cotton					1244:1249	cotton textile	1244:1257	cotton textile	1244:1257	A tensile strength analysis of the composite showed a Young's modulus approximately 1 MPa higher than that of cotton textile.					
34201854	7	33	theme	green	1499:1503	arg1	procedures					1506:1515	"green" procedures	1498:1515	"green" procedures	1498:1515	This work is an approach to demonstrate the potential of these polysaccharides, modified by "green" procedures to remove pollutants from water.					
34201854	6	34	theme	circa	1349:1353	arg1	adsorption					1264:1273	The adsorption	1260:1273	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C	1260:1343	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	6	34	theme	circa	1349:1353	arg1	%					1357:1357	circa 74%	1349:1357	circa 74%	1349:1357	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	6	35	theme	contact	1372:1378	arg1	h					1367:1367	6 h	1365:1367	6 h of contact	1365:1378	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	7	36	theme	polysaccharides	1469:1483	arg1	potential					1450:1458	the potential	1446:1458	the potential of these polysaccharides, modified by "green" procedures to remove pollutants from water	1446:1547	This work is an approach to demonstrate the potential of these polysaccharides, modified by "green" procedures to remove pollutants from water.					
34201854	6	37	from	°C	1342:1343	arg1	adsorption					1264:1273	The adsorption	1260:1273	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C	1260:1343	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	6	37	from	°C	1342:1343	arg1	%					1357:1357	circa 74%	1349:1357	circa 74%	1349:1357	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	3	38	theme	lead	901:904	arg1	Pb					912:913	Pb	912:913	Pb	912:913	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	38	theme	lead	901:904	arg1	ions					906:909	lead ions	901:909	lead ions (Pb)	901:914	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	2	39	theme	cotton-chitosan	424:438	arg1	catalyst					558:565	a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2	556:647	a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2	556:647	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	2	39	theme	cotton-chitosan	424:438	arg1	CCs					452:454	CCs	452:454	CCs	452:454	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	2	39	theme	cotton-chitosan	424:438	arg1	composites					440:449	cotton-chitosan composites	424:449	cotton-chitosan composites (CCs)	424:455	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	2	39	theme	cotton-chitosan	424:438	arg1	adsorbents					391:400	adsorbents	391:400	adsorbents for heavy metals	391:417	Polysaccharides such as cellulose have received attention as adsorbents for heavy metals, and cotton-chitosan composites (CCs) were developed here with nontoxic reagents such as carboxylic acids as crosslinkers and NaH2PO4 as a catalyst to achieve chitosan covalent crosslinkage into oxidized cotton textiles with H2O2.					
34201854	1	40	dep	processes	213:221	arg1	adsorption					261:270	adsorption	261:270	adsorption	261:270	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	40	dep	processes	213:221	arg1	use					230:232	use	230:232	can use to address this issue	226:254	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	40	dep	processes	213:221	arg1	popular					284:290	popular	284:290	popular	284:290	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	3	41	theme	ions	906:909	arg1	adsorption					887:896	the adsorption	883:896	the adsorption of lead ions (Pb)	883:914	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	0	42	theme	Cotton-Chitosan	42:56	arg1	Composite					58:66	a Cotton-Chitosan Composite	40:66	a Cotton-Chitosan Composite for Lead Removal from Water	40:94	The Development and Characterization of a Cotton-Chitosan Composite for Lead Removal from Water.					
34201854	4	43	theme	textile	1125:1131	arg1	cotton					1118:1123	cotton textile	1118:1131	cotton textile	1118:1131	The composites showed a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile.					
34201854	3	44	theme	scanning	817:824	arg1	microscopy					835:844	atomic-force and scanning electron microscopy	800:844	microscopy	835:844	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	4	45	theme	cotton	1118:1123	arg1	gram					1110:1113	gram	1110:1113	gram of cotton textile	1110:1131	The composites showed a maximum incorporation of chitosan of 27.62 mg per gram of cotton textile.					
34201854	1	46	from	metals	103:108	arg1	water					113:117	water	113:117	water	113:117	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	47	theme	several	205:211	arg1	processes					213:221	several processes	205:221	several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency	205:327	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	6	48	from	pH	1330:1331	arg1	adsorption					1264:1273	The adsorption	1260:1273	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C	1260:1343	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	6	48	from	pH	1330:1331	arg1	%					1357:1357	circa 74%	1349:1357	circa 74%	1349:1357	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	3	49	theme	atomic-force	800:811	arg1	microscopy					835:844	atomic-force and scanning electron microscopy	800:844	microscopy	835:844	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	7	50	from	water	1543:1547	arg1	pollutants					1527:1536	pollutants	1527:1536	pollutants from water	1527:1547	This work is an approach to demonstrate the potential of these polysaccharides, modified by "green" procedures to remove pollutants from water.					
34201854	0	51	theme	Lead	72:75	arg1	Removal					77:83	Lead Removal	72:83	Lead Removal from Water	72:94	The Development and Characterization of a Cotton-Chitosan Composite for Lead Removal from Water.					
34201854	0	52	from	Water	90:94	arg1	Removal					77:83	Lead Removal	72:83	Lead Removal from Water	72:94	The Development and Characterization of a Cotton-Chitosan Composite for Lead Removal from Water.					
34201854	0	53	dep	Development	4:14	arg1	The					0:2	The	0:2	The	0:2	The Development and Characterization of a Cotton-Chitosan Composite for Lead Removal from Water.					
34201854	1	54	theme	serious	125:131	arg1	problem					147:153	a serious environmental problem	123:153	a serious environmental problem due to their accumulation and toxicity	123:192	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	54	theme	serious	125:131	arg1	metals					103:108	Heavy metals	97:108	Heavy metals in water	97:117	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	3	55	theme	electron	826:833	arg1	microscopy					835:844	atomic-force and scanning electron microscopy	800:844	microscopy	835:844	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	0	56	theme	Composite	58:66	arg1	Characterization					20:35	Characterization	20:35	Characterization	20:35	The Development and Characterization of a Cotton-Chitosan Composite for Lead Removal from Water.					
34201854	0	56	theme	Composite	58:66	arg1	Development					4:14	Development	4:14	Development	4:14	The Development and Characterization of a Cotton-Chitosan Composite for Lead Removal from Water.					
34201854	6	57	from	composites	1293:1302	arg1	solution					1318:1325	an aqueous solution	1307:1325	an aqueous solution	1307:1325	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	5	58	theme	composite	1169:1177	arg1	analysis					1153:1160	A tensile strength analysis	1134:1160	A tensile strength analysis of the composite	1134:1177	A tensile strength analysis of the composite showed a Young's modulus approximately 1 MPa higher than that of cotton textile.					
34201854	6	59	with	ions	1283:1286	arg1	composites					1293:1302	composites	1293:1302	composites in an aqueous solution	1293:1325	The adsorption of lead ions with composites in an aqueous solution at pH 5 and 25 °C was circa 74% after 6 h of contact, as determined by MP-AES.					
34201854	1	60	theme	environmental	133:145	arg1	problem					147:153	a serious environmental problem	123:153	a serious environmental problem due to their accumulation and toxicity	123:192	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	1	60	theme	environmental	133:145	arg1	metals					103:108	Heavy metals	97:108	Heavy metals in water	97:117	Heavy metals in water are a serious environmental problem due to their accumulation and toxicity; there are several processes we can use to address this issue, but adsorption is the most popular due to its simplicity and efficiency.					
34201854	3	61	theme	X-ray	760:764	arg1	XPS					794:796	XPS	794:796	XPS	794:796	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
34201854	3	61	theme	X-ray	760:764	arg1	spectroscopy					780:791	X-ray photoelectron spectroscopy	760:791	X-ray photoelectron spectroscopy (XPS)	760:797	The composites were characterized by fourier-transform infrared spectroscopy (FTIR), elemental analysis (EA), X-ray photoelectron spectroscopy (XPS), atomic-force and scanning electron microscopy (AFM and SEM), and tensile strength; the adsorption of lead ions (Pb) was evaluated with cotton-chitosan composites and quantified by microwave plasma atomic emission spectroscopy (MP-AES).					
33321134	1	0	theme	dye	317:319	arg1	sequestration					297:309	the sequestration	293:309	the sequestration of MO dye	293:319	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	4	1	theme	MO	654:655	arg1	sequestration					661:673	MO dye sequestration	654:673	MO dye sequestration	654:673	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	8	2	from	adjustable	1324:1333	arg1	assemblies					1361:1370	the water purification assemblies	1338:1370	the water purification assemblies	1338:1370	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	7	3	theme	thermodynamic	1045:1057	arg1	process					1059:1065	thermodynamic process	1045:1065	thermodynamic process	1045:1065	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	8	4	from	assemblies	1361:1370	arg1	adjustable					1324:1333	adjustable	1324:1333	adjustable	1324:1333	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	2	5	theme	as-synthesized	326:339	arg1	films					341:345	The as-synthesized films	322:345	The as-synthesized films	322:345	The as-synthesized films were characterized by using FSESM, XPS XRD, and TGA analysis.					
33321134	4	6	from	power	645:649	arg1	presence					682:689	the presence	678:689	the presence of CSZ3	678:697	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	0	7	theme	composites	100:109	arg1	films					111:115	chitosan composites films	91:115	chitosan composites films	91:115	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	7	8	from	adsorption	1153:1162	arg1	exterior					1181:1188	the exterior	1177:1188	the exterior of films	1177:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	6	9	used	used	918:921	arg2	models					906:911	The Freundlich and Langmuir adsorption models	867:911	The Freundlich and Langmuir adsorption models	867:911	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	0	10	theme	chitosan	91:98	arg1	films					111:115	chitosan composites films	91:115	chitosan composites films	91:115	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	7	11	theme	dye	1170:1172	arg1	adsorption					1153:1162	the adsorption	1149:1162	the adsorption of MO dye on the exterior of films	1149:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	3	12	theme	pH	506:507	arg1	effect					509:514	pH effect	506:514	pH effect	506:514	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	7	13	theme	endothermic	1110:1120	arg1	process					1138:1144	the endothermic and spontaneous process	1106:1144	the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films	1106:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	4	14	theme	sequestration	661:673	arg1	higher					714:719	higher	714:719	higher	714:719	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	4	14	theme	sequestration	661:673	arg1	power					645:649	The adsorption power	630:649	The adsorption power of MO dye sequestration in the presence of CSZ3	630:697	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	6	15	theme	dye	1014:1016	arg1	adsorption					997:1006	multilayered adsorption	984:1006	multilayered adsorption of MO dye	984:1016	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	3	16	theme	orange	437:442	arg1	MO					449:450	MO	449:450	MO	449:450	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	3	16	theme	orange	437:442	arg1	dye					444:446	methyl orange dye	430:446	methyl orange dye (MO)	430:451	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	3	17	theme	adsorption	479:488	arg1	parameters					490:499	various adsorption parameters	471:499	various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration	471:606	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	6	18	theme	multilayered	984:995	arg1	adsorption					997:1006	multilayered adsorption	984:1006	multilayered adsorption of MO dye	984:1016	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	6	19	theme	data	965:968	arg1	analysis					940:947	the analysis	936:947	the analysis of experimental data that revealed multilayered adsorption of MO dye	936:1016	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	7	20	from	exterior	1181:1188	arg1	process					1138:1144	the endothermic and spontaneous process	1106:1144	the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films	1106:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	5	21	theme	chemical	848:855	arg1	kinetics					857:864	chemical kinetics	848:864	chemical kinetics	848:864	The experimental data is fitted in the pseudo-second order of chemical kinetics.					
33321134	6	22	theme	MO	1011:1012	arg1	dye					1014:1016	MO dye	1011:1016	MO dye	1011:1016	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	1	23	theme	chitosan	168:175	arg1	composites					200:209	chitosan coated sodium zeolites composites	168:209	chitosan coated sodium zeolites composites	168:209	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	6	24	theme	Freundlich	871:880	arg1	models					906:911	The Freundlich and Langmuir adsorption models	867:911	The Freundlich and Langmuir adsorption models	867:911	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	7	25	theme	adsorption	1153:1162	arg1	process					1138:1144	the endothermic and spontaneous process	1106:1144	the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films	1106:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	3	26	theme	reaction	517:524	arg1	temperature					526:536	reaction temperature	517:536	reaction temperature	517:536	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	1	27	theme	coated	177:182	arg1	composites					200:209	chitosan coated sodium zeolites composites	168:209	chitosan coated sodium zeolites composites	168:209	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	0	28	theme	Adsorption	0:9	arg1	studies					40:46	Adsorption, kinetics and thermodynamics studies	0:46	Adsorption, kinetics and thermodynamics studies of methyl orange dye	0:67	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	6	29	theme	adsorption	895:904	arg1	models					906:911	The Freundlich and Langmuir adsorption models	867:911	The Freundlich and Langmuir adsorption models	867:911	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	8	30	theme	present	1204:1210	arg1	work					1212:1215	The present work	1200:1215	The present work	1200:1215	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	8	30	theme	present	1204:1210	arg1	general					1220:1226	general	1220:1226	general	1220:1226	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	5	31	theme	kinetics	857:864	arg1	order					839:843	the pseudo-second order	821:843	the pseudo-second order of chemical kinetics	821:864	The experimental data is fitted in the pseudo-second order of chemical kinetics.					
33321134	1	32	theme	sodium	184:189	arg1	composites					200:209	chitosan coated sodium zeolites composites	168:209	chitosan coated sodium zeolites composites	168:209	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	6	33	theme	Langmuir	886:893	arg1	adsorption					895:904	Langmuir adsorption	886:904	Langmuir adsorption	886:904	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	8	34	theme	scale	1287:1291	arg1	applications					1293:1304	large scale applications	1281:1304	large scale applications	1281:1304	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	8	35	theme	large	1281:1285	arg1	applications					1293:1304	large scale applications	1281:1304	large scale applications	1281:1304	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	1	36	theme	zeolites	191:198	arg1	composites					200:209	chitosan coated sodium zeolites composites	168:209	chitosan coated sodium zeolites composites	168:209	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	2	37	theme	TGA	395:397	arg1	analysis					399:406	TGA analysis	395:406	TGA analysis	395:406	The as-synthesized films were characterized by using FSESM, XPS XRD, and TGA analysis.					
33321134	8	38	theme	water	1342:1346	arg1	assemblies					1361:1370	the water purification assemblies	1338:1370	the water purification assemblies	1338:1370	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	3	39	dep	effect	509:514	arg1	i.e.					501:504	i.e.	501:504	i.e.	501:504	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	4	40	theme	CSZ3	694:697	arg1	presence					682:689	the presence	678:689	the presence of CSZ3	678:697	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	1	41	theme	composites	200:209	arg1	films					211:215	pure chitosan (CS) and chitosan coated sodium zeolites composites films	145:215	pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively	145:268	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	5	42	theme	pseudo-second	825:837	arg1	order					839:843	the pseudo-second order	821:843	the pseudo-second order of chemical kinetics	821:864	The experimental data is fitted in the pseudo-second order of chemical kinetics.					
33321134	7	43	from	process	1138:1144	arg1	exterior					1181:1188	the exterior	1177:1188	the exterior of films	1177:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	5	44	theme	experimental	790:801	arg1	data					803:806	The experimental data	786:806	The experimental data	786:806	The experimental data is fitted in the pseudo-second order of chemical kinetics.					
33321134	6	45	theme	experimental	952:963	arg1	data					965:968	experimental data	952:968	experimental data that revealed multilayered adsorption of MO dye	952:1016	The Freundlich and Langmuir adsorption models were used on behalf of the analysis of experimental data that revealed multilayered adsorption of MO dye.					
33321134	0	46	theme	thermodynamics	25:38	arg1	studies					40:46	Adsorption, kinetics and thermodynamics studies	0:46	Adsorption, kinetics and thermodynamics studies of methyl orange dye	0:67	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	3	47	theme	original	581:588	arg1	concentration					594:606	original dye concentration	581:606	original dye concentration	581:606	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	2	48	theme	XPS	382:384	arg1	XRD					386:388	XPS XRD	382:388	XPS XRD	382:388	The as-synthesized films were characterized by using FSESM, XPS XRD, and TGA analysis.					
33321134	0	49	theme	methyl	51:56	arg1	dye					65:67	methyl orange dye	51:67	methyl orange dye	51:67	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	7	50	theme	spontaneous	1126:1136	arg1	process					1138:1144	the endothermic and spontaneous process	1106:1144	the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films	1106:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	3	51	theme	dye	590:592	arg1	concentration					594:606	original dye concentration	581:606	original dye concentration	581:606	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	1	52	theme	Different	118:126	arg1	films					128:132	Different films	118:132	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively	118:268	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	3	53	theme	catalytic	539:547	arg1	dosage					549:554	catalytic dosage	539:554	catalytic dosage	539:554	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	7	54	theme	films	1193:1197	arg1	exterior					1181:1188	the exterior	1177:1188	the exterior of films	1177:1197	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
33321134	4	55	theme	dye	657:659	arg1	sequestration					661:673	MO dye sequestration	654:673	MO dye sequestration	654:673	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	8	56	theme	purification	1348:1359	arg1	assemblies					1361:1370	the water purification assemblies	1338:1370	the water purification assemblies	1338:1370	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	4	57	theme	adsorption	634:643	arg1	higher					714:719	higher	714:719	higher	714:719	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	4	57	theme	adsorption	634:643	arg1	power					645:649	The adsorption power	630:649	The adsorption power of MO dye sequestration in the presence of CSZ3	630:697	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	3	58	theme	dye	444:446	arg1	sequestration					413:425	The sequestration	409:425	The sequestration of methyl orange dye (MO)	409:451	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	3	59	theme	batch	611:615	arg1	experiments					617:627	batch experiments	611:627	batch experiments	611:627	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	4	60	theme	fine	730:733	arg1	201 mg g-1					739:748	201 mg g-1	739:748	201 mg g-1	739:748	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	4	60	theme	fine	730:733	arg1	CS					735:736	the fine CS	726:736	the fine CS (201 mg g-1)	726:749	The adsorption power of MO dye sequestration in the presence of CSZ3 was 287 mg g-1 higher than the fine CS (201 mg g-1), and lowest for CSZ4 (173 mg g-1).					
33321134	1	61	theme	pure	145:148	arg1	CS					160:161	CS	160:161	CS	160:161	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	1	61	theme	pure	145:148	arg1	chitosan					150:157	pure chitosan	145:157	pure chitosan (CS)	145:162	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	8	62	theme	MO	1236:1237	arg1	adsorption					1239:1248	the MO adsorption	1232:1248	the MO adsorption	1232:1248	The present work is general for the MO adsorption, however, it can be applied on large scale applications and can be easily adjustable in the water purification assemblies.					
33321134	0	63	theme	dye	65:67	arg1	studies					40:46	Adsorption, kinetics and thermodynamics studies	0:46	Adsorption, kinetics and thermodynamics studies of methyl orange dye	0:67	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	3	64	theme	interaction	557:567	arg1	period					569:574	interaction period	557:574	interaction period	557:574	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	3	65	theme	methyl	430:435	arg1	MO					449:450	MO	449:450	MO	449:450	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	3	65	theme	methyl	430:435	arg1	dye					444:446	methyl orange dye	430:446	methyl orange dye (MO)	430:451	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	1	66	theme	chitosan	150:157	arg1	films					211:215	pure chitosan (CS) and chitosan coated sodium zeolites composites films	145:215	pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively	145:268	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	0	67	theme	orange	58:63	arg1	dye					65:67	methyl orange dye	51:67	methyl orange dye	51:67	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	1	68	theme	MO	314:315	arg1	dye					317:319	MO dye	314:319	MO dye	314:319	Different films comprising pure chitosan (CS) and chitosan coated sodium zeolites composites films designated as CSZ1, CSZ2, CSZ3 and CSZ4 respectively are presented here for the sequestration of MO dye.					
33321134	0	69	theme	kinetics	12:19	arg1	studies					40:46	Adsorption, kinetics and thermodynamics studies	0:46	Adsorption, kinetics and thermodynamics studies of methyl orange dye	0:67	Adsorption, kinetics and thermodynamics studies of methyl orange dye sequestration through chitosan composites films.					
33321134	3	70	theme	various	471:477	arg1	parameters					490:499	various adsorption parameters	471:499	various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration	471:606	The sequestration of methyl orange dye (MO) was studied under various adsorption parameters i.e. pH effect, reaction temperature, catalytic dosage, interaction period, and original dye concentration in batch experiments.					
33321134	7	71	theme	MO	1167:1168	arg1	dye					1170:1172	MO dye	1167:1172	MO dye	1167:1172	Kinetics, equilibrium and thermodynamic process were discussed in detailed, suggesting the endothermic and spontaneous process of the adsorption of MO dye on the exterior of films.					
32784924	0	0	theme	Polish	85:90	arg1	Market					92:97	the Polish Market	81:97	the Polish Market	81:97	Sweetening Agents and Sweeteners in Dietary Supplements for Children-Analysis of the Polish Market.					
32784924	7	1	theme	products	1050:1057	arg1	policy					1094:1099	policy	1094:1099	policy	1094:1099	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	7	1	theme	products	1050:1057	arg1	rates					1035:1039	high consumption rates	1018:1039	high consumption rates of these products as well as limited regulations and policy	1018:1099	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	7	1	theme	products	1050:1057	arg1	regulations					1078:1088	limited regulations	1070:1088	limited regulations	1070:1088	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	7	2	theme	composition	970:980	arg1	analysis					954:961	analysis	954:961	analysis of the composition of DS	954:986	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	1	3	theme	major	142:146	arg1	agents					111:116	Sweetening agents	100:116	Sweetening agents (SA)	100:121	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	1	3	theme	major	142:146	arg1	sweeteners					127:136	sweeteners	127:136	sweeteners	127:136	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	1	3	theme	major	142:146	arg1	additives					148:156	major additives	142:156	major additives used in the production of dietary supplements (DS)	142:207	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	6	4	theme	target	871:876	arg1	group					878:882	the target group	867:882	the target group	867:882	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	6	5	theme	products	798:805	arg1	formulation					783:793	the formulation	779:793	the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements	779:930	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	7	6	theme	regulations	1078:1088	arg1	light					1009:1013	the light	1005:1013	the light of high consumption rates of these products as well as limited regulations and policy	1005:1099	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	6	7	theme	empirical	692:700	arg1	findings					702:709	The empirical findings	688:709	The empirical findings	688:709	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	3	8	theme	representative	475:488	arg1	samples					490:496	representative samples	475:496	representative samples of DS (N = 315)	475:512	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	1	9	theme	technological	228:240	arg1	functions					259:267	both technological and organoleptic functions	223:267	both technological and organoleptic functions	223:267	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	0	10	theme	Market	92:97	arg1	Children-Analysis					60:76	Children-Analysis	60:76	Children-Analysis of the Polish Market	60:97	Sweetening Agents and Sweeteners in Dietary Supplements for Children-Analysis of the Polish Market.					
32784924	3	11	theme	samples	490:496	arg1	documentation					458:470	the documentation	454:470	the documentation of representative samples of DS (N = 315)	454:512	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	7	12	theme	consumption	1023:1033	arg1	rates					1035:1039	high consumption rates	1018:1039	high consumption rates of these products as well as limited regulations and policy	1018:1099	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	7	13	theme	rates	1035:1039	arg1	light					1009:1013	the light	1005:1013	the light of high consumption rates of these products as well as limited regulations and policy	1005:1099	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	2	14	theme	secondary	393:401	arg1	role					403:406	the secondary role	389:406	the secondary role of them	389:414	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	7	15	theme	crucial	937:943	arg1	need					945:948	The crucial need	933:948	The crucial need for analysis of the composition of DS	933:986	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	6	16	theme	consumption	842:852	arg1	abilities					854:862	consumption abilities	842:862	consumption abilities of the target group	842:882	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	2	17	theme	study	286:290	arg1	aim					274:276	The aim	270:276	The aim of this study	270:290	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	7	18	theme	high	1018:1021	arg1	rates					1035:1039	high consumption rates	1018:1039	high consumption rates of these products as well as limited regulations and policy	1018:1099	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	1	19	theme	organoleptic	246:257	arg1	functions					259:267	both technological and organoleptic functions	223:267	both technological and organoleptic functions	223:267	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	2	20	theme	sweeteners	327:336	arg1	types					311:315	the types	307:315	the types of SA and sweeteners found in DS intended for children	307:370	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	1	21	theme	Sweetening	100:109	arg1	additives					148:156	major additives	142:156	major additives used in the production of dietary supplements (DS)	142:207	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	1	21	theme	Sweetening	100:109	arg1	SA					119:120	SA	119:120	SA	119:120	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	1	21	theme	Sweetening	100:109	arg1	sweeteners					127:136	sweeteners	127:136	sweeteners	127:136	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	1	21	theme	Sweetening	100:109	arg1	agents					111:116	Sweetening agents	100:116	Sweetening agents (SA)	100:121	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	3	22	theme	DS	501:502	arg1	samples					490:496	representative samples	475:496	representative samples of DS (N = 315)	475:512	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	1	23	theme	dietary	184:190	arg1	DS					205:206	DS	205:206	DS	205:206	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	1	23	theme	dietary	184:190	arg1	supplements					192:202	dietary supplements	184:202	dietary supplements (DS)	184:207	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	0	24	theme	Dietary	36:42	arg1	Supplements					44:54	Dietary Supplements	36:54	Dietary Supplements for Children-Analysis of the Polish Market	36:97	Sweetening Agents and Sweeteners in Dietary Supplements for Children-Analysis of the Polish Market.					
32784924	6	25	theme	ingredient	744:753	arg1	type					725:728	the type	721:728	the type of sweetening ingredient	721:753	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	6	26	theme	taste	913:917	arg1	requirements					919:930	the children's taste requirements	898:930	the children's taste requirements	898:930	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	1	27	theme	supplements	192:202	arg1	production					170:179	the production	166:179	the production of dietary supplements (DS)	166:207	Sweetening agents (SA) and sweeteners are major additives used in the production of dietary supplements (DS), they fulfill both technological and organoleptic functions.					
32784924	2	28	theme	them	411:414	arg1	role					403:406	the secondary role	389:406	the secondary role of them	389:414	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	6	29	theme	sweetening	733:742	arg1	ingredient					744:753	sweetening ingredient	733:753	sweetening ingredient	733:753	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
32784924	3	30	theme	available	514:522	arg1	data					444:447	data	444:447	data from the documentation of representative samples of DS (N = 315) available on the Polish market	444:543	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	5	31	theme	DS	673:674	arg1	production					676:685	DS production	673:685	DS production	673:685	Sucrose is the SA most frequently used in DS production.					
32784924	3	32	theme	=	507:507	arg1	DS					501:502	DS	501:502	DS (N = 315)	501:512	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	3	32	theme	=	507:507	arg1	N					505:505	N = 315	505:511	N = 315	505:511	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	7	33	theme	limited	1070:1076	arg1	regulations					1078:1088	limited regulations	1070:1088	limited regulations	1070:1088	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	3	34	theme	Polish	531:536	arg1	market					538:543	the Polish market	527:543	the Polish market	527:543	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	2	35	located	found	338:342	arg1	DS					347:348	DS	347:348	DS intended for children	347:370	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	2	35	located	found	338:342	arg2	SA					320:321	SA	320:321	SA	320:321	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	4	36	theme	products	582:589	arg1	%					573:573	75.24%	568:573	75.24% of the products	568:589	The results show that 75.24% of the products contained at least one SA or sweetener.					
32784924	4	36	theme	products	582:589	arg1	products					582:589	the products	578:589	the products	578:589	The results show that 75.24% of the products contained at least one SA or sweetener.					
32784924	7	37	theme	DS	985:986	arg1	composition					970:980	the composition	966:980	the composition of DS	966:986	The crucial need for analysis of the composition of DS is emphasized in the light of high consumption rates of these products as well as limited regulations and policy.					
32784924	4	38	contain	contained	591:599	arg2	sweetener					620:628	sweetener	620:628	sweetener	620:628	The results show that 75.24% of the products contained at least one SA or sweetener.					
32784924	4	38	contain	contained	591:599	arg2	SA					614:615	SA	614:615	SA	614:615	The results show that 75.24% of the products contained at least one SA or sweetener.					
32784924	4	38	contain	contained	591:599	arg1	%					573:573	75.24%	568:573	75.24% of the products	568:589	The results show that 75.24% of the products contained at least one SA or sweetener.					
32784924	4	38	contain	contained	591:599	arg1	products					582:589	the products	578:589	the products	578:589	The results show that 75.24% of the products contained at least one SA or sweetener.					
32784924	2	39	theme	SA	320:321	arg1	types					311:315	the types	307:315	the types of SA and sweeteners found in DS intended for children	307:370	The aim of this study is to identify the types of SA and sweeteners found in DS intended for children and to determine the secondary role of them.					
32784924	3	40	from	documentation	458:470	arg1	data					444:447	data	444:447	data from the documentation of representative samples of DS (N = 315) available on the Polish market	444:543	The study was performed on data from the documentation of representative samples of DS (N = 315) available on the Polish market.					
32784924	6	41	theme	group	878:882	arg1	abilities					854:862	consumption abilities	842:862	consumption abilities of the target group	842:882	The empirical findings show that the type of sweetening ingredient correlates closely with the formulation of products, which in turn has to be suited to consumption abilities of the target group as well as to the children's taste requirements.					
33940026	1	0	theme	precorneal	333:342	arg1	period					354:359	precorneal residence period	333:359	precorneal residence period	333:359	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	8	1	theme	AZM	1306:1308	arg1	drops					1321:1325	AZM commercial drops	1306:1325	AZM commercial drops	1306:1325	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	1	2	theme	chitosan	251:258	arg1	system					311:316	a novel colloidal system	293:316	a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability	293:396	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	2	theme	chitosan	251:258	arg1	AZM-CTS-NSM					277:287	AZM-CTS-NSM	277:287	AZM-CTS-NSM	277:287	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	2	theme	chitosan	251:258	arg1	niosomes					267:274	azithromycin chitosan coated niosomes	238:274	azithromycin chitosan coated niosomes (AZM-CTS-NSM)	238:288	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	3	3	theme	corneal	681:687	arg1	membrane					697:704	the corneal mucosal membrane	677:704	the corneal mucosal membrane	677:704	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	1	4	theme	residence	344:352	arg1	period					354:359	precorneal residence period	333:359	precorneal residence period	333:359	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	5	theme	present	173:179	arg1	study					181:185	the present study	169:185	the present study	169:185	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	6	theme	coated	260:265	arg1	system					311:316	a novel colloidal system	293:316	a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability	293:396	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	6	theme	coated	260:265	arg1	AZM-CTS-NSM					277:287	AZM-CTS-NSM	277:287	AZM-CTS-NSM	277:287	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	6	theme	coated	260:265	arg1	niosomes					267:274	azithromycin chitosan coated niosomes	238:274	azithromycin chitosan coated niosomes (AZM-CTS-NSM)	238:288	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	0	7	theme	Permeable	105:113	arg1	Nanocarrier					136:146	A Highly Permeable and Efficient Ocular Nanocarrier	96:146	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	4	8	theme	optimized	767:775	arg1	AZM-CTS-NSM					777:787	The resulting optimized AZM-CTS-NSM	753:787	The resulting optimized AZM-CTS-NSM	753:787	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	3	9	from	influence	508:516	arg1	diameter					613:620	particle diameter	604:620	particle diameter	604:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	10	theme	surfactant	538:547	arg1	ratio					555:559	the cholesterol: surfactant molar ratio	521:559	the cholesterol: surfactant molar ratio	521:559	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	4	11	contain	has	789:791	arg2	diameter					800:807	a mean diameter	793:807	a mean diameter of 376 nm	793:817	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	11	contain	has	789:791	arg2	force					885:889	mucoadhesion force	872:889	mucoadhesion force of 3114 dyne/cm2	872:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	11	contain	has	789:791	arg1	AZM-CTS-NSM					777:787	The resulting optimized AZM-CTS-NSM	753:787	The resulting optimized AZM-CTS-NSM	753:787	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	11	contain	has	789:791	arg2	entrapment					820:829	entrapment	820:829	entrapment of 74.2%	820:838	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	11	contain	has	789:791	arg2	charge					849:854	surface charge	841:854	surface charge of 32.1 mV	841:865	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	12	theme	%	838:838	arg1	entrapment					820:829	entrapment	820:829	entrapment of 74.2%	820:838	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	12	theme	%	838:838	arg1	charge					849:854	surface charge	841:854	surface charge of 32.1 mV	841:865	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	12	theme	%	838:838	arg1	force					885:889	mucoadhesion force	872:889	mucoadhesion force of 3114 dyne/cm2	872:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	12	theme	%	838:838	arg1	diameter					800:807	a mean diameter	793:807	a mean diameter of 376 nm	793:817	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	7	13	theme	major	1226:1230	arg1	effects					1241:1247	no major irritant effects	1223:1247	no major irritant effects found to be induced by the formulated NSM	1223:1289	Additionally, ocular irritation was assessed, with no major irritant effects found to be induced by the formulated NSM.					
33940026	3	14	theme	molar	549:553	arg1	ratio					555:559	the cholesterol: surfactant molar ratio	521:559	the cholesterol: surfactant molar ratio	521:559	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	7	15	theme	formulated	1276:1285	arg1	NSM					1287:1289	the formulated NSM	1272:1289	the formulated NSM	1272:1289	Additionally, ocular irritation was assessed, with no major irritant effects found to be induced by the formulated NSM.					
33940026	0	16	theme	Efficient	119:127	arg1	Nanocarrier					136:146	A Highly Permeable and Efficient Ocular Nanocarrier	96:146	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	1	17	theme	eye	362:364	arg1	permeation					366:375	eye permeation	362:375	eye permeation	362:375	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	4	18	theme	376 nm	812:817	arg1	entrapment					820:829	entrapment	820:829	entrapment of 74.2%	820:838	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	18	theme	376 nm	812:817	arg1	charge					849:854	surface charge	841:854	surface charge of 32.1 mV	841:865	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	18	theme	376 nm	812:817	arg1	force					885:889	mucoadhesion force	872:889	mucoadhesion force of 3114 dyne/cm2	872:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	18	theme	376 nm	812:817	arg1	diameter					800:807	a mean diameter	793:807	a mean diameter of 376 nm	793:817	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	3	19	theme	cholesterol	525:535	arg1	ratio					555:559	the cholesterol: surfactant molar ratio	521:559	the cholesterol: surfactant molar ratio	521:559	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	6	20	theme	optimized	1032:1040	arg1	AZM-CTS-NSM					1042:1052	the optimized AZM-CTS-NSM	1028:1052	the optimized AZM-CTS-NSM	1028:1052	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	3	21	from	potential	640:648	arg1	diameter					613:620	particle diameter	604:620	particle diameter	604:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	22	theme	mucosal	689:695	arg1	membrane					697:704	the corneal mucosal membrane	677:704	the corneal mucosal membrane	677:704	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	23	from	force	668:672	arg1	diameter					613:620	particle diameter	604:620	particle diameter	604:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	7	24	theme	ocular	1186:1191	arg1	irritation					1193:1202	ocular irritation	1186:1202	ocular irritation	1186:1202	Additionally, ocular irritation was assessed, with no major irritant effects found to be induced by the formulated NSM.					
33940026	0	25	theme	Novel	0:4	arg1	Formulation					31:41	Novel Chitosan-Coated Niosomal Formulation	0:41	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	8	26	from	increase	1372:1379	arg1	eyes					1416:1419	the rabbit eyes	1405:1419	the rabbit eyes	1405:1419	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	8	26	from	increase	1372:1379	arg1	concentration					1388:1400	AZM concentration	1384:1400	AZM concentration	1384:1400	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	0	27	theme	Chitosan-Coated	6:20	arg1	Formulation					31:41	Novel Chitosan-Coated Niosomal Formulation	0:41	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	3	28	theme	CTS	562:564	arg1	concentration					566:578	CTS concentration	562:578	CTS concentration	562:578	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	2	29	theme	hydration	447:455	arg1	strategy					457:464	a modified thin-film hydration strategy	426:464	a modified thin-film hydration strategy	426:464	AZM-NSM was formulated via a modified thin-film hydration strategy and then coated with CTS.					
33940026	3	30	theme	composite	729:737	arg1	CCD					747:749	CCD	747:749	CCD	747:749	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	30	theme	composite	729:737	arg1	design					739:744	a central composite design	719:744	a central composite design (CCD)	719:750	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	31	theme	concentration	566:578	arg1	influence					508:516	the influence	504:516	the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter	504:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	31	theme	concentration	566:578	arg1	force					668:672	NSM adhesion force	655:672	NSM adhesion force	655:672	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	31	theme	concentration	566:578	arg1	potential					640:648	zeta potential	635:648	zeta potential	635:648	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	31	theme	concentration	566:578	arg1	entrapment					623:632	entrapment	623:632	entrapment	623:632	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	2	32	theme	thin-film	437:445	arg1	strategy					457:464	a modified thin-film hydration strategy	426:464	a modified thin-film hydration strategy	426:464	AZM-NSM was formulated via a modified thin-film hydration strategy and then coated with CTS.					
33940026	8	33	theme	rabbit	1409:1414	arg1	eyes					1416:1419	the rabbit eyes	1405:1419	the rabbit eyes	1405:1419	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	4	34	theme	mean	795:798	arg1	diameter					800:807	a mean diameter	793:807	a mean diameter of 376 nm	793:817	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	3	35	theme	ratio	555:559	arg1	influence					508:516	the influence	504:516	the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter	504:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	35	theme	ratio	555:559	arg1	force					668:672	NSM adhesion force	655:672	NSM adhesion force	655:672	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	35	theme	ratio	555:559	arg1	potential					640:648	zeta potential	635:648	zeta potential	635:648	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	35	theme	ratio	555:559	arg1	entrapment					623:632	entrapment	623:632	entrapment	623:632	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	5	36	theme	release	969:975	arg1	behavior					977:984	a prolonged in vitro release behavior	948:984	a prolonged in vitro release behavior	948:984	The optimized AZM-CTS-NSM demonstrated a prolonged in vitro release behavior.					
33940026	5	37	dep	in	960:961	arg1	vitro					963:967	vitro	963:967	vitro	963:967	The optimized AZM-CTS-NSM demonstrated a prolonged in vitro release behavior.					
33940026	2	38	theme	modified	428:435	arg1	strategy					457:464	a modified thin-film hydration strategy	426:464	a modified thin-film hydration strategy	426:464	AZM-NSM was formulated via a modified thin-film hydration strategy and then coated with CTS.					
33940026	9	39	theme	valuable	1490:1497	arg1	CTS-NSM					1465:1471	CTS-NSM	1465:1471	CTS-NSM	1465:1471	Collectively, these findings indicate that CTS-NSM is a potentially valuable ocular nanocarrier that could augment the efficacy of AZM.					
33940026	9	39	theme	valuable	1490:1497	arg1	nanocarrier					1506:1516	a potentially valuable ocular nanocarrier	1476:1516	a potentially valuable ocular nanocarrier that could augment the efficacy of AZM	1476:1555	Collectively, these findings indicate that CTS-NSM is a potentially valuable ocular nanocarrier that could augment the efficacy of AZM.					
33940026	0	40	theme	Ocular	129:134	arg1	Nanocarrier					136:146	A Highly Permeable and Efficient Ocular Nanocarrier	96:146	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	3	41	theme	zeta	635:638	arg1	potential					640:648	zeta potential	635:648	zeta potential	635:648	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	6	42	theme	permeability	1100:1111	arg1	coefficient					1113:1123	the apparent permeability coefficient	1087:1123	the apparent permeability coefficient	1087:1123	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	6	43	theme	commercial	1006:1015	arg1	drops					1021:1025	commercial eye drops	1006:1025	commercial eye drops	1006:1025	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	0	44	theme	Improved	47:54	arg1	Management					56:65	Improved Management	47:65	Improved Management of Bacterial Conjunctivitis	47:93	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	3	45	theme	central	721:727	arg1	CCD					747:749	CCD	747:749	CCD	747:749	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	45	theme	central	721:727	arg1	design					739:744	a central composite design	719:744	a central composite design (CCD)	719:750	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	7	46	theme	irritant	1232:1239	arg1	effects					1241:1247	no major irritant effects	1223:1247	no major irritant effects found to be induced by the formulated NSM	1223:1289	Additionally, ocular irritation was assessed, with no major irritant effects found to be induced by the formulated NSM.					
33940026	3	47	theme	surfactant	585:594	arg1	type					596:599	surfactant type	585:599	surfactant type	585:599	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	1	48	theme	novel	295:299	arg1	niosomes					267:274	azithromycin chitosan coated niosomes	238:274	azithromycin chitosan coated niosomes (AZM-CTS-NSM)	238:288	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	48	theme	novel	295:299	arg1	system					311:316	a novel colloidal system	293:316	a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability	293:396	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	4	49	theme	mucoadhesion	872:883	arg1	force					885:889	mucoadhesion force	872:889	mucoadhesion force of 3114 dyne/cm2	872:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	8	50	theme	commercial	1310:1319	arg1	drops					1321:1325	AZM commercial drops	1306:1325	AZM commercial drops	1306:1325	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	3	51	theme	type	596:599	arg1	influence					508:516	the influence	504:516	the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter	504:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	51	theme	type	596:599	arg1	force					668:672	NSM adhesion force	655:672	NSM adhesion force	655:672	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	51	theme	type	596:599	arg1	potential					640:648	zeta potential	635:648	zeta potential	635:648	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	51	theme	type	596:599	arg1	entrapment					623:632	entrapment	623:632	entrapment	623:632	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	4	52	theme	dyne/cm2	899:906	arg1	entrapment					820:829	entrapment	820:829	entrapment of 74.2%	820:838	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	52	theme	dyne/cm2	899:906	arg1	charge					849:854	surface charge	841:854	surface charge of 32.1 mV	841:865	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	52	theme	dyne/cm2	899:906	arg1	force					885:889	mucoadhesion force	872:889	mucoadhesion force of 3114 dyne/cm2	872:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	52	theme	dyne/cm2	899:906	arg1	diameter					800:807	a mean diameter	793:807	a mean diameter of 376 nm	793:817	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	1	53	theme	colloidal	301:309	arg1	niosomes					267:274	azithromycin chitosan coated niosomes	238:274	azithromycin chitosan coated niosomes (AZM-CTS-NSM)	238:288	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	53	theme	colloidal	301:309	arg1	system					311:316	a novel colloidal system	293:316	a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability	293:396	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	5	54	theme	optimized	913:921	arg1	AZM-CTS-NSM					923:933	The optimized AZM-CTS-NSM	909:933	The optimized AZM-CTS-NSM	909:933	The optimized AZM-CTS-NSM demonstrated a prolonged in vitro release behavior.					
33940026	6	55	from	increase	1075:1082	arg1	coefficient					1113:1123	the apparent permeability coefficient	1087:1123	the apparent permeability coefficient	1087:1123	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	6	56	theme	apparent	1091:1098	arg1	coefficient					1113:1123	the apparent permeability coefficient	1087:1123	the apparent permeability coefficient	1087:1123	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	8	57	theme	AZM	1384:1386	arg1	concentration					1388:1400	AZM concentration	1384:1400	AZM concentration	1384:1400	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	4	58	theme	surface	841:847	arg1	charge					849:854	surface charge	841:854	surface charge of 32.1 mV	841:865	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	3	59	theme	NSM	655:657	arg1	force					668:672	NSM adhesion force	655:672	NSM adhesion force	655:672	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	4	60	theme	3114	894:897	arg1	dyne/cm2					899:906	3114 dyne/cm2	894:906	3114 dyne/cm2	894:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	9	61	theme	AZM	1553:1555	arg1	efficacy					1541:1548	the efficacy	1537:1548	the efficacy of AZM	1537:1555	Collectively, these findings indicate that CTS-NSM is a potentially valuable ocular nanocarrier that could augment the efficacy of AZM.					
33940026	3	62	from	entrapment	623:632	arg1	diameter					613:620	particle diameter	604:620	particle diameter	604:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	3	63	theme	particle	604:611	arg1	diameter					613:620	particle diameter	604:620	particle diameter	604:620	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	4	64	theme	32.1 mV	859:865	arg1	entrapment					820:829	entrapment	820:829	entrapment of 74.2%	820:838	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	64	theme	32.1 mV	859:865	arg1	charge					849:854	surface charge	841:854	surface charge of 32.1 mV	841:865	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	64	theme	32.1 mV	859:865	arg1	force					885:889	mucoadhesion force	872:889	mucoadhesion force of 3114 dyne/cm2	872:906	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	4	64	theme	32.1 mV	859:865	arg1	diameter					800:807	a mean diameter	793:807	a mean diameter of 376 nm	793:817	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	3	65	theme	adhesion	659:666	arg1	force					668:672	NSM adhesion force	655:672	NSM adhesion force	655:672	We assessed the influence of the cholesterol: surfactant molar ratio, CTS concentration, and surfactant type on particle diameter, entrapment, zeta potential, and NSM adhesion force to the corneal mucosal membrane and employed a central composite design (CCD).					
33940026	0	66	dep	Formulation	31:41	arg1	Nanocarrier					136:146	A Highly Permeable and Efficient Ocular Nanocarrier	96:146	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	8	67	theme	3-fold	1365:1370	arg1	increase					1372:1379	˃ 3-fold increase	1363:1379	˃ 3-fold increase in AZM concentration in the rabbit eyes	1363:1419	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	5	68	theme	prolonged	950:958	arg1	behavior					977:984	a prolonged in vitro release behavior	948:984	a prolonged in vitro release behavior	948:984	The optimized AZM-CTS-NSM demonstrated a prolonged in vitro release behavior.					
33940026	8	69	theme	optimized	1332:1340	arg1	AZM-CTS-NSM					1342:1352	the optimized AZM-CTS-NSM	1328:1352	the optimized AZM-CTS-NSM	1328:1352	Compared with AZM commercial drops, the optimized AZM-CTS-NSM revealed ˃ 3-fold increase in AZM concentration in the rabbit eyes.					
33940026	4	70	theme	resulting	757:765	arg1	AZM-CTS-NSM					777:787	The resulting optimized AZM-CTS-NSM	753:787	The resulting optimized AZM-CTS-NSM	753:787	The resulting optimized AZM-CTS-NSM has a mean diameter of 376 nm, entrapment of 74.2%, surface charge of 32.1 mV, and mucoadhesion force of 3114 dyne/cm2.					
33940026	0	71	theme	Conjunctivitis	80:93	arg1	Management					56:65	Improved Management	47:65	Improved Management of Bacterial Conjunctivitis	47:93	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	5	72	theme	in	960:961	arg1	behavior					977:984	a prolonged in vitro release behavior	948:984	a prolonged in vitro release behavior	948:984	The optimized AZM-CTS-NSM demonstrated a prolonged in vitro release behavior.					
33940026	6	73	theme	eye	1017:1019	arg1	drops					1021:1025	commercial eye drops	1006:1025	commercial eye drops	1006:1025	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	6	74	theme	2.61-fold	1065:1073	arg1	increase					1075:1082	a 2.61-fold increase	1063:1082	a 2.61-fold increase in the apparent permeability coefficient	1063:1123	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	6	75	theme	corneal	1150:1156	arg1	permeability					1158:1169	corneal permeability	1150:1169	corneal permeability	1150:1169	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	6	76	dep	produced	1054:1061	arg1	improving					1140:1148	improving	1140:1148	significantly improving corneal permeability	1126:1169	When compared with commercial eye drops, the optimized AZM-CTS-NSM produced a 2.61-fold increase in the apparent permeability coefficient, significantly improving corneal permeability.					
33940026	0	77	theme	Bacterial	70:78	arg1	Conjunctivitis					80:93	Bacterial Conjunctivitis	70:93	Bacterial Conjunctivitis	70:93	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	0	78	theme	Niosomal	22:29	arg1	Formulation					31:41	Novel Chitosan-Coated Niosomal Formulation	0:41	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.	0:164	Novel Chitosan-Coated Niosomal Formulation for Improved Management of Bacterial Conjunctivitis: A Highly Permeable and Efficient Ocular Nanocarrier for Azithromycin.					
33940026	9	79	theme	ocular	1499:1504	arg1	CTS-NSM					1465:1471	CTS-NSM	1465:1471	CTS-NSM	1465:1471	Collectively, these findings indicate that CTS-NSM is a potentially valuable ocular nanocarrier that could augment the efficacy of AZM.					
33940026	9	79	theme	ocular	1499:1504	arg1	nanocarrier					1506:1516	a potentially valuable ocular nanocarrier	1476:1516	a potentially valuable ocular nanocarrier that could augment the efficacy of AZM	1476:1555	Collectively, these findings indicate that CTS-NSM is a potentially valuable ocular nanocarrier that could augment the efficacy of AZM.					
33940026	1	80	theme	azithromycin	238:249	arg1	system					311:316	a novel colloidal system	293:316	a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability	293:396	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	80	theme	azithromycin	238:249	arg1	AZM-CTS-NSM					277:287	AZM-CTS-NSM	277:287	AZM-CTS-NSM	277:287	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
33940026	1	80	theme	azithromycin	238:249	arg1	niosomes					267:274	azithromycin chitosan coated niosomes	238:274	azithromycin chitosan coated niosomes (AZM-CTS-NSM)	238:288	In the present study, we aimed to formulate, optimize, and characterize azithromycin chitosan coated niosomes (AZM-CTS-NSM) as a novel colloidal system that increases precorneal residence period, eye permeation, and bioavailability.					
32270772	10	0	theme	Akkermansia	1334:1344	arg1	level					1325:1329	a higher level	1316:1329	a higher level of Akkermansia	1316:1344	Additionally, the sequencing analysis showed that the stachyose changed the gut microbiota composition with a higher level of Akkermansia, as well as selectively increasing some probiotics, including Lactobacillus.					
32270772	4	1	theme	control	547:553	arg1	group					555:559	(1) control group	543:559	(1) control group	543:559	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	5	2	theme	Eosin	666:670	arg1	staining					682:689	Hemotoxylin and Eosin (H and E) staining	650:689	Hemotoxylin and Eosin (H and E) staining	650:689	Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage.					
32270772	6	3	theme	inflammatory	777:788	arg1	IL-10					816:820	IL-10	816:820	IL-10	816:820	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	6	3	theme	inflammatory	777:788	arg1	IL-6					810:813	IL-6	810:813	IL-6	810:813	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	6	3	theme	inflammatory	777:788	arg1	cytokines					790:798	The inflammatory cytokines	773:798	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum	773:848	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	6	3	theme	inflammatory	777:788	arg1	IL-17a					823:828	IL-17a	823:828	IL-17a	823:828	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	6	3	theme	inflammatory	777:788	arg1	TNF-α					835:839	TNF-α	835:839	TNF-α	835:839	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	10	4	theme	gut	1284:1286	arg1	composition					1299:1309	the gut microbiota composition	1280:1309	the gut microbiota composition	1280:1309	Additionally, the sequencing analysis showed that the stachyose changed the gut microbiota composition with a higher level of Akkermansia, as well as selectively increasing some probiotics, including Lactobacillus.					
32270772	9	5	theme	upregulated	1105:1115	arg1	cytokines					1130:1138	the upregulated inflammatory cytokines	1101:1138	the upregulated inflammatory cytokines induced by DSS	1101:1153	And the upregulated inflammatory cytokines induced by DSS were significantly inhibited by stachyose treatment.					
32270772	11	6	dep	increased	1472:1480	arg1	which					1564:1568	which	1564:1568	which	1564:1568	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	11	6	dep	increased	1472:1480	arg1	option					1595:1600	a new promising option	1579:1600	a new promising option to UC patients	1579:1615	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	4	7	dep	MATERIALS	457:465	arg1	divided					517:523	divided	517:523	were randomly divided into four groups	503:540	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	11	8	theme	beneficial	1482:1491	arg1	microbiota					1493:1502	beneficial microbiota	1482:1502	beneficial microbiota	1482:1502	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	4	9	dep	group	616:620	arg1	3					584:584	3	584:584	3	584:584	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	4	10	theme	DSS	639:641	arg1	group					643:647	DSS group	639:647	DSS group	639:647	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	8	11	theme	stachyose	1006:1014	arg1	treatment					1016:1024	the stachyose treatment	1002:1024	the stachyose treatment	1002:1024	RESULTS Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis.					
32270772	3	12	from	effects	371:377	arg1	levels					408:413	inflammatory levels	395:413	inflammatory levels	395:413	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	3	12	from	effects	371:377	arg1	microbiota					423:432	gut microbiota	419:432	gut microbiota	419:432	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	10	13	theme	higher	1318:1323	arg1	level					1325:1329	a higher level	1316:1329	a higher level of Akkermansia	1316:1344	Additionally, the sequencing analysis showed that the stachyose changed the gut microbiota composition with a higher level of Akkermansia, as well as selectively increasing some probiotics, including Lactobacillus.					
32270772	9	14	theme	stachyose	1187:1195	arg1	treatment					1197:1205	stachyose treatment	1187:1205	stachyose treatment	1187:1205	And the upregulated inflammatory cytokines induced by DSS were significantly inhibited by stachyose treatment.					
32270772	7	15	theme	rDNA	941:944	arg1	sequencing					951:960	rDNA gene sequencing	941:960	rDNA gene sequencing	941:960	The differences in the gut microbiota were analyzed by 16S rDNA gene sequencing.					
32270772	1	16	theme	imbalanced	166:175	arg1	flora					185:189	imbalanced enteric flora	166:189	imbalanced enteric flora	166:189	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	8	17	theme	DSS-induced	1076:1086	arg1	colitis					1088:1094	DSS-induced colitis	1076:1094	DSS-induced colitis	1076:1094	RESULTS Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis.					
32270772	7	18	theme	gene	946:949	arg1	sequencing					951:960	rDNA gene sequencing	941:960	rDNA gene sequencing	941:960	The differences in the gut microbiota were analyzed by 16S rDNA gene sequencing.					
32270772	8	19	dep	RESULTS	963:969	arg1	showed					990:995	showed	990:995	showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis	990:1094	RESULTS Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis.					
32270772	3	20	theme	colitis	443:449	arg1	mice					451:454	acute colitis mice	437:454	acute colitis mice	437:454	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	3	21	theme	stachyose	382:390	arg1	effects					371:377	the effects	367:377	the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice	367:454	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	6	22	from	cytokines	790:798	arg1	serum					844:848	serum	844:848	serum	844:848	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	3	23	theme	present	333:339	arg1	study					341:345	The present study	329:345	The present study	329:345	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	2	24	theme	microbiota	317:326	arg1	composition					294:304	the composition	290:304	the composition of the gut microbiota	290:326	Stachyose is a kind of natural prebiotic which favorably modulate the composition of the gut microbiota.					
32270772	11	25	theme	new	1581:1583	arg1	which					1564:1568	which	1564:1568	which	1564:1568	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	11	25	theme	new	1581:1583	arg1	option					1595:1600	a new promising option	1579:1600	a new promising option to UC patients	1579:1615	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	4	26	theme	sodium	603:608	arg1	group					616:620	(3) dextran sulfate sodium (DSS) group	583:620	(3) dextran sulfate sodium (DSS) group	583:620	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	8	27	from	lesions	1052:1058	arg1	colitis					1088:1094	DSS-induced colitis	1076:1094	DSS-induced colitis	1076:1094	RESULTS Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis.					
32270772	4	28	theme	dextran	587:593	arg1	group					616:620	(3) dextran sulfate sodium (DSS) group	583:620	(3) dextran sulfate sodium (DSS) group	583:620	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	2	29	theme	gut	313:315	arg1	microbiota					317:326	the gut microbiota	309:326	the gut microbiota	309:326	Stachyose is a kind of natural prebiotic which favorably modulate the composition of the gut microbiota.					
32270772	4	30	theme	sulfate	595:601	arg1	group					616:620	(3) dextran sulfate sodium (DSS) group	583:620	(3) dextran sulfate sodium (DSS) group	583:620	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	5	31	theme	distal	713:718	arg1	colon					720:724	the distal colon	709:724	the distal colon	709:724	Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage.					
32270772	10	32	theme	microbiota	1288:1297	arg1	composition					1299:1309	the gut microbiota composition	1280:1309	the gut microbiota composition	1280:1309	Additionally, the sequencing analysis showed that the stachyose changed the gut microbiota composition with a higher level of Akkermansia, as well as selectively increasing some probiotics, including Lactobacillus.					
32270772	1	33	theme	BACKGROUND/AIM	104:117	arg1	UC					139:140	UC	139:140	UC	139:140	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	1	33	theme	BACKGROUND/AIM	104:117	arg1	colitis					130:136	BACKGROUND/AIM Ulcerative colitis	104:136	BACKGROUND/AIM Ulcerative colitis (UC)	104:141	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	1	34	theme	reduced	195:201	arg1	diversity					213:221	reduced microbial diversity	195:221	reduced microbial diversity	195:221	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	0	35	theme	gut	20:22	arg1	microbiota					24:33	gut microbiota	20:33	gut microbiota	20:33	Stachyose modulates gut microbiota and alleviates dextran sulfate sodium-induced acute colitis in mice.					
32270772	1	36	theme	Ulcerative	119:128	arg1	UC					139:140	UC	139:140	UC	139:140	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	1	36	theme	Ulcerative	119:128	arg1	colitis					130:136	BACKGROUND/AIM Ulcerative colitis	104:136	BACKGROUND/AIM Ulcerative colitis (UC)	104:141	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	1	37	theme	microbial	203:211	arg1	diversity					213:221	reduced microbial diversity	195:221	reduced microbial diversity	195:221	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	7	38	theme	gut	905:907	arg1	microbiota					909:918	the gut microbiota	901:918	the gut microbiota	901:918	The differences in the gut microbiota were analyzed by 16S rDNA gene sequencing.					
32270772	0	39	from	colitis	87:93	arg1	mice					98:101	mice	98:101	mice	98:101	Stachyose modulates gut microbiota and alleviates dextran sulfate sodium-induced acute colitis in mice.					
32270772	11	40	theme	UC	1605:1606	arg1	patients					1608:1615	UC patients	1605:1615	UC patients	1605:1615	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	11	41	from	colitis	1547:1553	arg1	mice					1558:1561	mice	1558:1561	mice	1558:1561	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	3	42	theme	mice	451:454	arg1	levels					408:413	inflammatory levels	395:413	inflammatory levels	395:413	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	3	42	theme	mice	451:454	arg1	microbiota					423:432	gut microbiota	419:432	gut microbiota	419:432	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	3	43	theme	acute	437:441	arg1	colitis					443:449	acute colitis	437:449	acute colitis mice	437:454	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	3	44	theme	gut	419:421	arg1	microbiota					423:432	gut microbiota	419:432	gut microbiota	419:432	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	11	45	theme	promising	1585:1593	arg1	which					1564:1568	which	1564:1568	which	1564:1568	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	11	45	theme	promising	1585:1593	arg1	option					1595:1600	a new promising option	1579:1600	a new promising option to UC patients	1579:1615	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	6	46	theme	ELISA	869:873	arg1	assay					875:879	ELISA assay	869:879	ELISA assay	869:879	The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay.					
32270772	10	47	theme	sequencing	1226:1235	arg1	analysis					1237:1244	the sequencing analysis	1222:1244	the sequencing analysis	1222:1244	Additionally, the sequencing analysis showed that the stachyose changed the gut microbiota composition with a higher level of Akkermansia, as well as selectively increasing some probiotics, including Lactobacillus.					
32270772	3	48	theme	inflammatory	395:406	arg1	levels					408:413	inflammatory levels	395:413	inflammatory levels	395:413	The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.					
32270772	8	49	theme	colon	1067:1071	arg1	lesions					1052:1058	the lesions	1048:1058	the lesions of the colon in DSS-induced colitis	1048:1094	RESULTS Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis.					
32270772	0	50	theme	sodium-induced	66:79	arg1	colitis					87:93	dextran sulfate sodium-induced acute colitis	50:93	dextran sulfate sodium-induced acute colitis in mice	50:101	Stachyose modulates gut microbiota and alleviates dextran sulfate sodium-induced acute colitis in mice.					
32270772	11	51	dep	CONCLUSIONS	1423:1433	arg1	suggested					1447:1455	suggested	1447:1455	suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients	1447:1615	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	0	52	theme	dextran	50:56	arg1	sulfate					58:64	dextran sulfate	50:64	dextran sulfate sodium-induced acute colitis in mice	50:101	Stachyose modulates gut microbiota and alleviates dextran sulfate sodium-induced acute colitis in mice.					
32270772	9	53	theme	inflammatory	1117:1128	arg1	cytokines					1130:1138	the upregulated inflammatory cytokines	1101:1138	the upregulated inflammatory cytokines induced by DSS	1101:1153	And the upregulated inflammatory cytokines induced by DSS were significantly inhibited by stachyose treatment.					
32270772	4	54	dep	group	576:580	arg1	2					563:563	2	563:563	2	563:563	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	11	55	theme	bacterial	1508:1516	arg1	diversity					1518:1526	bacterial diversity	1508:1526	bacterial diversity	1508:1526	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	5	56	dep	Hemotoxylin	650:660	arg1	E					679:679	E	679:679	E	679:679	Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage.					
32270772	5	56	dep	Hemotoxylin	650:660	arg1	H					673:673	H	673:673	H	673:673	Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage.					
32270772	8	57	theme	Histological	971:982	arg1	assay					984:988	Histological assay	971:988	Histological assay	971:988	RESULTS Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis.					
32270772	5	58	theme	Hemotoxylin	650:660	arg1	staining					682:689	Hemotoxylin and Eosin (H and E) staining	650:689	Hemotoxylin and Eosin (H and E) staining	650:689	Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage.					
32270772	4	59	dep	group	555:559	arg1	1					544:544	1	544:544	1	544:544	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	2	60	theme	prebiotic	255:263	arg1	Stachyose					224:232	Stachyose	224:232	Stachyose	224:232	Stachyose is a kind of natural prebiotic which favorably modulate the composition of the gut microbiota.					
32270772	2	60	theme	prebiotic	255:263	arg1	kind					239:242	a kind	237:242	a kind of natural prebiotic which favorably modulate the composition of the gut microbiota	237:326	Stachyose is a kind of natural prebiotic which favorably modulate the composition of the gut microbiota.					
32270772	11	61	theme	acute	1541:1545	arg1	colitis					1547:1553	acute colitis	1541:1553	acute colitis in mice	1541:1561	CONCLUSIONS Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.					
32270772	4	62	dep	stachyose	627:635	arg1	4					624:624	4	624:624	4	624:624	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	7	63	from	differences	886:896	arg1	microbiota					909:918	the gut microbiota	901:918	the gut microbiota	901:918	The differences in the gut microbiota were analyzed by 16S rDNA gene sequencing.					
32270772	0	64	theme	acute	81:85	arg1	colitis					87:93	dextran sulfate sodium-induced acute colitis	50:93	dextran sulfate sodium-induced acute colitis in mice	50:101	Stachyose modulates gut microbiota and alleviates dextran sulfate sodium-induced acute colitis in mice.					
32270772	2	65	theme	natural	247:253	arg1	prebiotic					255:263	natural prebiotic	247:263	natural prebiotic	247:263	Stachyose is a kind of natural prebiotic which favorably modulate the composition of the gut microbiota.					
32270772	4	66	theme	DSS	611:613	arg1	group					616:620	(3) dextran sulfate sodium (DSS) group	583:620	(3) dextran sulfate sodium (DSS) group	583:620	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	1	67	theme	enteric	177:183	arg1	flora					185:189	imbalanced enteric flora	166:189	imbalanced enteric flora	166:189	BACKGROUND/AIM Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity.					
32270772	4	68	theme	stachyose	566:574	arg1	group					576:580	(2) stachyose group	562:580	(2) stachyose group	562:580	MATERIALS AND METHODS In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group.					
32270772	0	69	theme	sulfate	58:64	arg1	colitis					87:93	dextran sulfate sodium-induced acute colitis	50:93	dextran sulfate sodium-induced acute colitis in mice	50:101	Stachyose modulates gut microbiota and alleviates dextran sulfate sodium-induced acute colitis in mice.					
32270772	5	70	theme	tissue	758:763	arg1	damage					765:770	tissue damage	758:770	tissue damage	758:770	Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage.					
32640016	6	0	theme	force	961:965	arg1	microscopy					967:976	Atomic force microscopy	954:976	Atomic force microscopy	954:976	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	9	1	theme	resulting	1491:1499	arg1	increases					1501:1509	The resulting increases	1487:1509	The resulting increases in cellulose and hemicellulose contents	1487:1549	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	1	2	theme	limited	236:242	arg1	data					244:247	limited data	236:247	limited data	236:247	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	6	3	theme	cellulose	1038:1046	arg1	fibrils					1048:1054	cellulose fibrils	1038:1054	cellulose fibrils	1038:1054	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	3	4	theme	genes	645:649	arg1	numerous					615:622	numerous	615:622	numerous	615:622	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	8	5	theme	fibrils	1441:1447	arg1	deposition					1417:1426	the deposition	1413:1426	the deposition of cellulose fibrils	1413:1447	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	2	6	theme	novel	318:322	arg1	mechanism					324:332	a novel mechanism	316:332	a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa)	316:439	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	7	7	theme	synthase	1180:1187	arg1	CSCs					1200:1203	CSCs	1200:1203	CSCs	1200:1203	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	7	7	theme	synthase	1180:1187	arg1	complexes					1189:1197	cellulose synthase complexes	1170:1197	cellulose synthase complexes (CSCs)	1170:1204	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	9	8	theme	cell	1583:1586	arg1	elongation					1588:1597	cell elongation	1583:1597	cell elongation	1583:1597	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	4	9	theme	cellulose	739:747	arg1	biosynthesis					723:734	the biosynthesis	719:734	the biosynthesis of cellulose and hemicellulose	719:765	These differentially expressed genes were directly associated with the biosynthesis of cellulose and hemicellulose, and with the degradation of pectin.					
32640016	9	10	from	increases	1501:1509	arg1	contents					1542:1549	cellulose and hemicellulose contents	1514:1549	cellulose and hemicellulose contents	1514:1549	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	7	11	theme	cellulose	1170:1178	arg1	CSCs					1200:1203	CSCs	1200:1203	CSCs	1200:1203	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	7	11	theme	cellulose	1170:1178	arg1	complexes					1189:1197	cellulose synthase complexes	1170:1197	cellulose synthase complexes (CSCs)	1170:1204	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	3	12	theme	epidermal	498:506	arg1	cells					508:512	epidermal cells	498:512	epidermal cells	498:512	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	5	13	theme	hemicellulose	905:917	arg1	contents					879:886	the contents	875:886	the contents of cellulose and hemicellulose	875:917	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	0	14	theme	fibrils	122:128	arg1	arrangement					97:107	the arrangement	93:107	the arrangement of cellulose fibrils in alfalfa	93:139	Hydrogen sulfide promotes hypocotyl elongation via increasing cellulose content and changing the arrangement of cellulose fibrils in alfalfa.					
32640016	8	15	theme	pectin	1471:1476	arg1	content					1478:1484	the pectin content	1467:1484	the pectin content	1467:1484	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	1	16	from	influence	270:278	arg1	walls					288:292	cell walls	283:292	cell walls	283:292	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	8	17	dep	enhances	1358:1365	arg1	regulating					1402:1411	regulating	1402:1411	regulating the deposition of cellulose fibrils	1402:1447	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	8	17	dep	enhances	1358:1365	arg1	decreasing					1456:1465	decreasing	1456:1465	decreasing the pectin content	1456:1484	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	4	18	theme	hemicellulose	753:765	arg1	biosynthesis					723:734	the biosynthesis	719:734	the biosynthesis of cellulose and hemicellulose	719:765	These differentially expressed genes were directly associated with the biosynthesis of cellulose and hemicellulose, and with the degradation of pectin.					
32640016	5	19	theme	composition	826:836	arg1	Analysis					804:811	Analysis	804:811	Analysis of cell wall composition	804:836	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	0	20	theme	cellulose	112:120	arg1	fibrils					122:128	cellulose fibrils	112:128	cellulose fibrils	112:128	Hydrogen sulfide promotes hypocotyl elongation via increasing cellulose content and changing the arrangement of cellulose fibrils in alfalfa.					
32640016	3	21	with	Treatment	442:450	arg1	NaHS					457:460	NaHS	457:460	NaHS	457:460	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	9	22	theme	cellulose	1514:1522	arg1	contents					1542:1549	cellulose and hemicellulose contents	1514:1549	cellulose and hemicellulose contents	1514:1549	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	7	23	theme	particles	1298:1306	arg1	mobility					1282:1289	mobility	1282:1289	mobility of the particles	1282:1306	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	8	24	theme	H2S	1347:1349	arg1	signal					1351:1356	the H2S signal	1343:1356	the H2S signal	1343:1356	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	7	25	theme	mobility	1282:1289	arg1	rate					1274:1277	the rate	1270:1277	the rate of mobility of the particles	1270:1306	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	0	26	theme	Hydrogen	0:7	arg1	sulfide					9:15	Hydrogen sulfide	0:15	Hydrogen sulfide	0:15	Hydrogen sulfide promotes hypocotyl elongation via increasing cellulose content and changing the arrangement of cellulose fibrils in alfalfa.					
32640016	6	27	theme	Atomic	954:959	arg1	microscopy					967:976	Atomic force microscopy	954:976	Atomic force microscopy	954:976	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	8	28	theme	cellulose	1431:1439	arg1	fibrils					1441:1447	cellulose fibrils	1431:1447	cellulose fibrils	1431:1447	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	9	29	theme	hemicellulose	1528:1540	arg1	contents					1542:1549	cellulose and hemicellulose contents	1514:1549	cellulose and hemicellulose contents	1514:1549	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	5	30	theme	NaHS	850:853	arg1	treatment					855:863	NaHS treatment	850:863	NaHS treatment	850:863	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	0	31	theme	hypocotyl	26:34	arg1	elongation					36:45	hypocotyl elongation	26:45	hypocotyl elongation	26:45	Hydrogen sulfide promotes hypocotyl elongation via increasing cellulose content and changing the arrangement of cellulose fibrils in alfalfa.					
32640016	8	32	theme	cell	1389:1392	arg1	wall					1394:1397	the cell wall	1385:1397	the cell wall	1385:1397	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	3	33	theme	cells	508:512	arg1	length					488:493	the length	484:493	the length of epidermal cells	484:512	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	2	34	theme	cell	392:395	arg1	cellulose					402:410	cell wall cellulose	392:410	cell wall cellulose	392:410	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	2	35	theme	wall	397:400	arg1	cellulose					402:410	cell wall cellulose	392:410	cell wall cellulose	392:410	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	1	36	contain	have	177:180	arg2	functions					205:213	positive physiological functions	182:213	positive physiological functions	182:213	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	1	36	contain	have	177:180	arg1	H2S					160:162	H2S	160:162	H2S	160:162	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	1	36	contain	have	177:180	arg1	sulfide					151:157	Hydrogen sulfide	142:157	Hydrogen sulfide (H2S)	142:163	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	1	37	theme	physiological	191:203	arg1	functions					205:213	positive physiological functions	182:213	positive physiological functions	182:213	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	5	38	theme	cellulose	891:899	arg1	contents					879:886	the contents	875:886	the contents of cellulose and hemicellulose	875:917	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	8	39	theme	wall	1394:1397	arg1	plasticity					1371:1380	the plasticity	1367:1380	the plasticity of the cell wall	1367:1397	Overall, our results suggest that the H2S signal enhances the plasticity of the cell wall by regulating the deposition of cellulose fibrils and by decreasing the pectin content.					
32640016	3	40	theme	transcriptome	536:548	arg1	analysis					550:557	transcriptome analysis	536:557	transcriptome analysis	536:557	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	6	41	theme	fibrils	1048:1054	arg1	diameter					1026:1033	the diameter	1022:1033	the diameter of cellulose fibrils	1022:1054	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	5	42	theme	cell	816:819	arg1	composition					826:836	cell wall composition	816:836	cell wall composition	816:836	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	1	43	theme	cell	283:286	arg1	walls					288:292	cell walls	283:292	cell walls	283:292	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	2	44	theme	cellulose	402:410	arg1	deposition					378:387	deposition	378:387	deposition	378:387	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	2	44	theme	cellulose	402:410	arg1	biosynthesis					361:372	biosynthesis	361:372	biosynthesis	361:372	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	5	45	theme	wall	821:824	arg1	composition					826:836	cell wall composition	816:836	cell wall composition	816:836	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	0	46	theme	cellulose	62:70	arg1	content					72:78	cellulose content	62:78	cellulose content	62:78	Hydrogen sulfide promotes hypocotyl elongation via increasing cellulose content and changing the arrangement of cellulose fibrils in alfalfa.					
32640016	9	47	theme	cell	1559:1562	arg1	expansion					1569:1577	cell wall expansion	1559:1577	cell wall expansion	1559:1577	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	3	48	theme	differential	588:599	arg1	expression					601:610	the differential expression	584:610	the differential expression of numerous of cell wall-related genes	584:649	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	9	49	theme	wall	1564:1567	arg1	expansion					1569:1577	cell wall expansion	1559:1577	cell wall expansion	1559:1577	The resulting increases in cellulose and hemicellulose contents lead to cell wall expansion and cell elongation.					
32640016	3	50	theme	cell	627:630	arg1	genes					645:649	cell wall-related genes	627:649	cell wall-related genes	627:649	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	4	51	theme	pectin	796:801	arg1	degradation					781:791	the degradation	777:791	the degradation of pectin	777:801	These differentially expressed genes were directly associated with the biosynthesis of cellulose and hemicellulose, and with the degradation of pectin.					
32640016	2	52	from	biosynthesis	361:372	arg1	alfalfa					415:421	alfalfa	415:421	alfalfa (Medicago sativa)	415:439	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	2	52	from	biosynthesis	361:372	arg1	sativa					433:438	Medicago sativa	424:438	Medicago sativa	424:438	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	4	53	theme	expressed	673:681	arg1	genes					683:687	These differentially expressed genes	652:687	These differentially expressed genes	652:687	These differentially expressed genes were directly associated with the biosynthesis of cellulose and hemicellulose, and with the degradation of pectin.					
32640016	3	54	theme	wall-related	632:643	arg1	genes					645:649	cell wall-related genes	627:649	cell wall-related genes	627:649	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	6	55	with	treatment	992:1000	arg1	NaHS					1007:1010	NaHS	1007:1010	NaHS	1007:1010	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	6	56	theme	bundles	1098:1104	arg1	arrangement					1069:1079	the arrangement	1065:1079	the arrangement of the fibrillar bundles	1065:1104	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	1	57	theme	plant	218:222	arg1	growth					224:229	plant growth	218:229	plant growth	218:229	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	3	58	theme	numerous	615:622	arg1	expression					601:610	the differential expression	584:610	the differential expression of numerous of cell wall-related genes	584:649	Treatment with NaHS was found to increase the length of epidermal cells in the hypocotyl, and transcriptome analysis indicated that it caused the differential expression of numerous of cell wall-related genes.					
32640016	5	59	theme	pectin	938:943	arg1	content					945:951	the pectin content	934:951	the pectin content	934:951	Analysis of cell wall composition showed that NaHS treatment increased the contents of cellulose and hemicellulose, but decreased the pectin content.					
32640016	2	60	from	deposition	378:387	arg1	alfalfa					415:421	alfalfa	415:421	alfalfa (Medicago sativa)	415:439	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	2	60	from	deposition	378:387	arg1	sativa					433:438	Medicago sativa	424:438	Medicago sativa	424:438	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	1	61	theme	Hydrogen	142:149	arg1	H2S					160:162	H2S	160:162	H2S	160:162	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	1	61	theme	Hydrogen	142:149	arg1	sulfide					151:157	Hydrogen sulfide	142:157	Hydrogen sulfide (H2S)	142:163	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	0	62	from	arrangement	97:107	arg1	alfalfa					133:139	alfalfa	133:139	alfalfa	133:139	Hydrogen sulfide promotes hypocotyl elongation via increasing cellulose content and changing the arrangement of cellulose fibrils in alfalfa.					
32640016	2	63	dep	biosynthesis	361:372	arg1	the					357:359	the	357:359	the	357:359	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	1	64	theme	positive	182:189	arg1	functions					205:213	positive physiological functions	182:213	positive physiological functions	182:213	Hydrogen sulfide (H2S) is known to have positive physiological functions in plant growth, but limited data are available on its influence on cell walls.					
32640016	6	65	theme	fibrillar	1088:1096	arg1	bundles					1098:1104	the fibrillar bundles	1084:1104	the fibrillar bundles	1084:1104	Atomic force microscopy revealed that treatment with NaHS decreased the diameter of cellulose fibrils, altered the arrangement of the fibrillar bundles, and increased the spacing between the bundles.					
32640016	2	66	theme	Medicago	424:431	arg1	alfalfa					415:421	alfalfa	415:421	alfalfa (Medicago sativa)	415:439	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	2	66	theme	Medicago	424:431	arg1	sativa					433:438	Medicago sativa	424:438	Medicago sativa	424:438	Here, we demonstrate a novel mechanism by which H2S regulates the biosynthesis and deposition of cell wall cellulose in alfalfa (Medicago sativa).					
32640016	7	67	theme	cellulose	1230:1238	arg1	synthesis					1240:1248	cellulose synthesis	1230:1248	cellulose synthesis	1230:1248	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	7	68	theme	complexes	1189:1197	arg1	related					1219:1225	related	1219:1225	related	1219:1225	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
32640016	7	68	theme	complexes	1189:1197	arg1	dynamics					1158:1165	The dynamics	1154:1165	The dynamics of cellulose synthase complexes (CSCs)	1154:1204	The dynamics of cellulose synthase complexes (CSCs) were closely related to cellulose synthesis, and NaHS increased the rate of mobility of the particles.					
33922589	7	0	theme	acid	1221:1224	arg1	levels					1199:1204	levels	1199:1204	levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid	1199:1287	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	5	1	theme	microbiota	816:825	arg1	Phascolarctobacterium					857:877	Phascolarctobacterium	857:877	Phascolarctobacterium	857:877	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	5	1	theme	microbiota	816:825	arg1	Faecalibacterium					836:851	Faecalibacterium	836:851	Faecalibacterium	836:851	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	5	1	theme	microbiota	816:825	arg1	abundances					791:800	the abundances	787:800	the abundances	787:800	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	6	2	theme	Bacterial	941:949	arg1	prediction					962:971	Bacterial functional prediction	941:971	Bacterial functional prediction	941:971	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	7	3	theme	acid	1239:1242	arg1	levels					1199:1204	levels	1199:1204	levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid	1199:1287	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	6	4	theme	bile	1058:1061	arg1	biosynthesis					1068:1079	secondary bile acid biosynthesis	1048:1079	secondary bile acid biosynthesis	1048:1079	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	0	5	theme	Short	90:94	arg1	Acids					108:112	Short Chain Fatty Acids	90:112	Short Chain Fatty Acids	90:112	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	7	6	theme	Targeted	1082:1089	arg1	metabolomics					1091:1102	Targeted metabolomics	1082:1102	Targeted metabolomics	1082:1102	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	3	7	from	functionality	460:472	arg1	rats					495:498	rats	495:498	rats	495:498	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	4	8	theme	normal	680:685	arg1	water					687:691	normal water	680:691	normal water	680:691	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	9	9	from	basis	1556:1560	arg1	use					1569:1571	the use	1565:1571	the use of isomaltulose as a prebiotic	1565:1602	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	0	10	from	Acids	134:138	arg1	Rats					143:146	Rats	143:146	Rats	143:146	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	7	11	theme	lithocholic	1209:1219	arg1	acid					1221:1224	lithocholic acid	1209:1224	lithocholic acid	1209:1224	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	6	12	theme	acid	1063:1066	arg1	biosynthesis					1068:1079	secondary bile acid biosynthesis	1048:1079	secondary bile acid biosynthesis	1048:1079	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	6	13	theme	functional	951:960	arg1	prediction					962:971	Bacterial functional prediction	941:971	Bacterial functional prediction	941:971	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	0	14	theme	Fatty	102:106	arg1	Acids					108:112	Short Chain Fatty Acids	90:112	Short Chain Fatty Acids	90:112	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	9	15	theme	bile	1508:1511	arg1	acids					1513:1517	secondary bile acids	1498:1517	secondary bile acids	1498:1517	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	9	16	theme	SCFAs	1488:1492	arg1	microbiota					1455:1464	gut microbiota	1451:1464	gut microbiota	1451:1464	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	9	16	theme	SCFAs	1488:1492	arg1	production					1474:1483	the production	1470:1483	the production of SCFAs and secondary bile acids	1470:1517	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	3	17	theme	microbiota	481:490	arg1	functionality					460:472	functionality	460:472	functionality	460:472	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	3	17	theme	microbiota	481:490	arg1	composition					444:454	composition	444:454	composition	444:454	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	5	18	theme	isomaltulose	764:775	arg1	ingestion					751:759	ingestion	751:759	ingestion of isomaltulose	751:775	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	0	19	theme	Chain	96:100	arg1	Acids					108:112	Short Chain Fatty Acids	90:112	Short Chain Fatty Acids	90:112	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	1	20	theme	In	149:150	arg1	experiments					158:168	In vitro experiments	149:168	In vitro experiments	149:168	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	4	21	theme	Sprague-Dawley	508:521	arg1	rats					523:526	Twelve Sprague-Dawley rats	501:526	Twelve Sprague-Dawley rats	501:526	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	7	22	theme	hyodeoxycholic	1269:1282	arg1	acid					1284:1287	hyodeoxycholic acid	1269:1287	hyodeoxycholic acid	1269:1287	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	4	23	theme	10	617:618	arg1	%					619:619	%	619:619	%	619:619	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	5	24	theme	16S	719:721	arg1	sequencing					728:737	16S rRNA sequencing	719:737	16S rRNA sequencing	719:737	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	1	25	theme	probiotics	252:261	arg1	growth					242:247	the growth	238:247	the growth of probiotics	238:261	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	4	26	theme	control	649:655	arg1	group					657:661	the control group	645:661	the control group	645:661	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	5	27	theme	rRNA	723:726	arg1	sequencing					728:737	16S rRNA sequencing	719:737	16S rRNA sequencing	719:737	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	3	28	from	composition	444:454	arg1	rats					495:498	rats	495:498	rats	495:498	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	9	29	theme	isomaltulose	1576:1587	arg1	use					1569:1571	the use	1565:1571	the use of isomaltulose as a prebiotic	1565:1602	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	6	30	theme	microbial	1011:1019	arg1	biosynthesis					1068:1079	secondary bile acid biosynthesis	1048:1079	secondary bile acid biosynthesis	1048:1079	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	6	30	theme	microbial	1011:1019	arg1	functionalities					1021:1035	gut microbial functionalities	1007:1035	gut microbial functionalities	1007:1035	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	0	31	theme	Prebiotic	22:30	arg1	Activity					32:39	Prebiotic Activity	22:39	Prebiotic Activity	22:39	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	0	32	theme	Acids	108:112	arg1	Acids					134:138	Secondary Bile Acids	119:138	Secondary Bile Acids in Rats	119:146	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	0	32	theme	Acids	108:112	arg1	Microbiota					60:69	Gut Microbiota	56:69	Gut Microbiota	56:69	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	0	32	theme	Acids	108:112	arg1	Production					76:85	the Production	72:85	the Production of Short Chain Fatty Acids	72:112	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	0	33	from	Production	76:85	arg1	Rats					143:146	Rats	143:146	Rats	143:146	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	7	34	theme	dehydrocholic	1245:1257	arg1	acid					1259:1262	dehydrocholic acid	1245:1262	dehydrocholic acid	1245:1262	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	9	35	theme	gut	1451:1453	arg1	microbiota					1455:1464	gut microbiota	1451:1464	gut microbiota	1451:1464	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	6	36	theme	gut	1007:1009	arg1	biosynthesis					1068:1079	secondary bile acid biosynthesis	1048:1079	secondary bile acid biosynthesis	1048:1079	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	6	36	theme	gut	1007:1009	arg1	functionalities					1021:1035	gut microbial functionalities	1007:1035	gut microbial functionalities	1007:1035	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	9	37	theme	acids	1513:1517	arg1	microbiota					1455:1464	gut microbiota	1451:1464	gut microbiota	1451:1464	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	9	37	theme	acids	1513:1517	arg1	production					1474:1483	the production	1470:1483	the production of SCFAs and secondary bile acids	1470:1517	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	0	38	theme	Secondary	119:127	arg1	Acids					134:138	Secondary Bile Acids	119:138	Secondary Bile Acids in Rats	119:146	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	7	39	theme	acid	1259:1262	arg1	levels					1199:1204	levels	1199:1204	levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid	1199:1287	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	8	40	theme	propionate	1322:1331	arg1	concentrations					1304:1317	the concentrations	1300:1317	the concentrations of propionate and butyrate	1300:1344	Moreover, the concentrations of propionate and butyrate were elevated in the rats administered with isomaltulose.					
33922589	1	41	theme	prebiotic	185:193	arg1	activity					195:202	prebiotic activity	185:202	prebiotic activity of isomaltulose, which stimulates the growth of probiotics	185:261	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	6	42	theme	secondary	1048:1056	arg1	biosynthesis					1068:1079	secondary bile acid biosynthesis	1048:1079	secondary bile acid biosynthesis	1048:1079	Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis.					
33922589	7	43	theme	cholic	1160:1165	arg1	acid					1167:1170	cholic acid	1160:1170	cholic acid concentration	1160:1184	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	9	44	theme	secondary	1498:1506	arg1	acids					1513:1517	secondary bile acids	1498:1517	secondary bile acids	1498:1517	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	7	45	theme	acid	1167:1170	arg1	concentration					1172:1184	cholic acid concentration	1160:1184	cholic acid concentration	1160:1184	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	2	46	theme	trials	350:355	arg1	absence					331:337	the absence	327:337	the absence of in vivo trials	327:355	However, the absence of in vivo trials undermines these results.					
33922589	0	47	theme	Gut	56:58	arg1	Microbiota					60:69	Gut Microbiota	56:69	Gut Microbiota	56:69	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	1	48	theme	short	285:289	arg1	SCFAs					310:314	SCFAs	310:314	SCFAs	310:314	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	1	48	theme	short	285:289	arg1	acids					303:307	short chain fatty acids	285:307	short chain fatty acids (SCFAs)	285:315	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	0	49	from	Microbiota	60:69	arg1	Rats					143:146	Rats	143:146	Rats	143:146	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	7	50	theme	acid	1284:1287	arg1	levels					1199:1204	levels	1199:1204	levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid	1199:1287	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	1	51	theme	chain	291:295	arg1	SCFAs					310:314	SCFAs	310:314	SCFAs	310:314	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	1	51	theme	chain	291:295	arg1	acids					303:307	short chain fatty acids	285:307	short chain fatty acids (SCFAs)	285:315	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	0	52	theme	Bile	129:132	arg1	Acids					134:138	Secondary Bile Acids	119:138	Secondary Bile Acids in Rats	119:146	Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats.					
33922589	4	53	contain	containing	606:615	arg1	water					600:604	water	600:604	water containing 10% isomaltulose (w/w)	600:638	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	4	53	contain	containing	606:615	arg2	w/w					635:637	w/w	635:637	w/w	635:637	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	4	53	contain	containing	606:615	arg2	isomaltulose					621:632	10% isomaltulose	617:632	10% isomaltulose (w/w)	617:638	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	1	54	dep	In	149:150	arg1	vitro					152:156	vitro	152:156	vitro	152:156	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	7	55	theme	deoxycholic	1227:1237	arg1	acid					1239:1242	deoxycholic acid	1227:1242	deoxycholic acid	1227:1242	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	1	56	theme	fatty	297:301	arg1	SCFAs					310:314	SCFAs	310:314	SCFAs	310:314	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	1	56	theme	fatty	297:301	arg1	acids					303:307	short chain fatty acids	285:307	short chain fatty acids (SCFAs)	285:315	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	3	57	from	effect	418:423	arg1	functionality					460:472	functionality	460:472	functionality	460:472	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	3	57	from	effect	418:423	arg1	composition					444:454	composition	444:454	composition	444:454	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	9	58	theme	scientific	1545:1554	arg1	basis					1556:1560	a scientific basis	1543:1560	a scientific basis on the use of isomaltulose as a prebiotic	1543:1602	This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.					
33922589	5	59	theme	pathogens	904:912	arg1	levels					894:899	levels	894:899	levels of pathogens, including Shuttleworthia	894:938	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	3	60	theme	isomaltulose	428:439	arg1	effect					418:423	the effect	414:423	the effect of isomaltulose on composition and functionality of gut microbiota in rats	414:498	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	8	61	theme	butyrate	1337:1344	arg1	concentrations					1304:1317	the concentrations	1300:1317	the concentrations of propionate and butyrate	1300:1344	Moreover, the concentrations of propionate and butyrate were elevated in the rats administered with isomaltulose.					
33922589	1	62	theme	isomaltulose	207:218	arg1	production					271:280	the production	267:280	the production of short chain fatty acids (SCFAs)	267:315	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	1	62	theme	isomaltulose	207:218	arg1	activity					195:202	prebiotic activity	185:202	prebiotic activity of isomaltulose, which stimulates the growth of probiotics	185:261	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	4	63	theme	%	619:619	arg1	w/w					635:637	w/w	635:637	w/w	635:637	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	4	63	theme	%	619:619	arg1	isomaltulose					621:632	10% isomaltulose	617:632	10% isomaltulose (w/w)	617:638	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	4	64	theme	free	585:588	arg1	access					590:595	free access	585:595	free access to water containing 10% isomaltulose (w/w)	585:638	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
33922589	5	65	theme	beneficial	805:814	arg1	microbiota					816:825	beneficial microbiota	805:825	beneficial microbiota	805:825	Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as Faecalibacterium and Phascolarctobacterium, and decreased levels of pathogens, including Shuttleworthia.					
33922589	2	66	theme	in	342:343	arg1	trials					350:355	in vivo trials	342:355	in vivo trials	342:355	However, the absence of in vivo trials undermines these results.					
33922589	7	67	theme	isomaltulose	1122:1133	arg1	supplementation					1135:1149	isomaltulose supplementation	1122:1149	isomaltulose supplementation	1122:1149	Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid.					
33922589	1	68	theme	acids	303:307	arg1	production					271:280	the production	267:280	the production of short chain fatty acids (SCFAs)	267:315	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	1	68	theme	acids	303:307	arg1	activity					195:202	prebiotic activity	185:202	prebiotic activity of isomaltulose, which stimulates the growth of probiotics	185:261	In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs).					
33922589	2	69	dep	in	342:343	arg1	vivo					345:348	vivo	345:348	vivo	345:348	However, the absence of in vivo trials undermines these results.					
33922589	3	70	theme	gut	477:479	arg1	microbiota					481:490	gut microbiota	477:490	gut microbiota	477:490	This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats.					
33922589	4	71	theme	IsoMTL	562:567	arg1	group					569:573	the IsoMTL group	558:573	the IsoMTL group	558:573	Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (w/w), and the control group was treated with normal water for five weeks.					
32871126	0	0	from	development	12:22	arg1	grain					96:100	wheat grain	90:100	wheat grain under nitrogen application	90:127	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	4	1	theme	external	816:823	arg1	region					825:830	the external region	812:830	the external region of starch granules	812:849	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	7	2	theme	starch	1369:1374	arg1	characteristics					1350:1364	the structural characteristics	1335:1364	the structural characteristics of starch	1335:1374	This study indicated that nitrogen application at booting stage significantly affected the structural characteristics of starch, and ultimately determines its quality.					
32871126	2	3	theme	starch	324:329	arg1	development					339:349	the starch granule development	320:349	the starch granule development	320:349	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	5	4	theme	starch	1033:1038	arg1	biosynthesis					1040:1051	starch biosynthesis	1033:1051	starch biosynthesis	1033:1051	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	6	5	theme	middle	1117:1122	arg1	stages					1148:1153	middle and later grain filling stages	1117:1153	middle and later grain filling stages	1117:1153	During middle and later grain filling stages, AGPase, GBSSI, and GBSSII were lower, and SSS, SBE, and DBE were higher in N240 than in N0.					
32871126	0	6	theme	granule	79:85	arg1	development					12:22	development	12:22	development	12:22	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	0	6	theme	granule	79:85	arg1	accumulation					25:36	accumulation	25:36	accumulation	25:36	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	0	6	theme	granule	79:85	arg1	characteristics					53:67	structural characteristics	42:67	structural characteristics	42:67	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	5	7	theme	different	1078:1086	arg1	stages					1102:1107	different developmental stages	1078:1107	different developmental stages	1078:1107	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	5	8	theme	mRNA	995:998	arg1	expression					1000:1009	the mRNA expression	991:1009	the mRNA expression of enzymes related to starch biosynthesis or degradation	991:1066	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	0	9	from	Analysis	0:7	arg1	grain					96:100	wheat grain	90:100	wheat grain under nitrogen application	90:127	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	4	10	theme	granules	720:727	arg1	distribution					697:708	distribution	697:708	distribution	697:708	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	10	theme	granules	720:727	arg1	composition					681:691	composition	681:691	composition	681:691	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	11	theme	wheat	921:925	arg1	development					927:937	wheat development	921:937	wheat development	921:937	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	0	12	theme	wheat	90:94	arg1	grain					96:100	wheat grain	90:100	wheat grain under nitrogen application	90:127	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	0	13	from	characteristics	53:67	arg1	grain					96:100	wheat grain	90:100	wheat grain under nitrogen application	90:127	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	6	14	theme	grain	1134:1138	arg1	stages					1148:1153	middle and later grain filling stages	1117:1153	middle and later grain filling stages	1117:1153	During middle and later grain filling stages, AGPase, GBSSI, and GBSSII were lower, and SSS, SBE, and DBE were higher in N240 than in N0.					
32871126	4	15	theme	starch	713:718	arg1	granules					720:727	starch granules	713:727	starch granules	713:727	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	0	16	theme	nitrogen	108:115	arg1	application					117:127	nitrogen application	108:127	nitrogen application	108:127	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	4	17	from	structure	799:807	arg1	region					825:830	the external region	812:830	the external region of starch granules	812:849	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	18	theme	ordered	791:797	arg1	structure					799:807	ordered structure	791:807	ordered structure	791:807	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	3	19	theme	amylose	508:514	arg1	biosynthesis					481:492	the biosynthesis	477:492	the biosynthesis of starch and amylose	477:514	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	3	20	from	proportion	578:587	arg1	endosperm					623:631	endosperm	623:631	endosperm	623:631	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	1	21	theme	important	158:166	arg1	nutrients					168:176	the most important nutrients	149:176	the most important nutrients for wheat growth	149:193	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	1	22	theme	nutrients	168:176	arg1	nutrients					168:176	the most important nutrients	149:176	the most important nutrients for wheat growth	149:193	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	1	22	theme	nutrients	168:176	arg1	one					142:144	one	142:144	one	142:144	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	0	23	from	accumulation	25:36	arg1	grain					96:100	wheat grain	90:100	wheat grain under nitrogen application	90:127	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	3	24	theme	starch	542:547	arg1	degradation					527:537	the degradation	523:537	the degradation of starch	523:547	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	2	25	theme	starch	352:357	arg1	accumulation					359:370	starch accumulation	352:370	starch accumulation	352:370	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	7	26	theme	structural	1339:1348	arg1	characteristics					1350:1364	the structural characteristics	1335:1364	the structural characteristics of starch	1335:1374	This study indicated that nitrogen application at booting stage significantly affected the structural characteristics of starch, and ultimately determines its quality.					
32871126	2	27	theme	nitrogen	284:291	arg1	240 kg/hm2					300:309	240 kg/hm2	300:309	240 kg/hm2	300:309	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	2	27	theme	nitrogen	284:291	arg1	level					293:297	medium nitrogen level	277:297	medium nitrogen level (240 kg/hm2)	277:310	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	1	28	theme	wheat	182:186	arg1	growth					188:193	wheat growth	182:193	wheat growth	182:193	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	2	29	theme	starch	413:418	arg1	accumulation					359:370	starch accumulation	352:370	starch accumulation	352:370	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	2	29	theme	starch	413:418	arg1	characteristics					388:402	structural characteristics	377:402	structural characteristics	377:402	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	2	29	theme	starch	413:418	arg1	development					339:349	the starch granule development	320:349	the starch granule development	320:349	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	5	30	theme	treatment	978:986	arg1	effect					959:964	The effect	955:964	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation	955:1066	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	2	31	theme	medium	277:282	arg1	240 kg/hm2					300:309	240 kg/hm2	300:309	240 kg/hm2	300:309	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	2	31	theme	medium	277:282	arg1	level					293:297	medium nitrogen level	277:297	medium nitrogen level (240 kg/hm2)	277:310	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	4	32	dep	lamellae	778:785	arg1	the					768:770	the	768:770	the	768:770	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	0	33	theme	development	12:22	arg1	Analysis					0:7	Analysis	0:7	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.	0:128	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	6	34	theme	filling	1140:1146	arg1	stages					1148:1153	middle and later grain filling stages	1117:1153	middle and later grain filling stages	1117:1153	During middle and later grain filling stages, AGPase, GBSSI, and GBSSII were lower, and SSS, SBE, and DBE were higher in N240 than in N0.					
32871126	6	35	theme	later	1128:1132	arg1	stages					1148:1153	middle and later grain filling stages	1117:1153	middle and later grain filling stages	1117:1153	During middle and later grain filling stages, AGPase, GBSSI, and GBSSII were lower, and SSS, SBE, and DBE were higher in N240 than in N0.					
32871126	3	36	theme	granule	612:618	arg1	proportion					578:587	the proportion	574:587	the proportion of B-type small starch granule in endosperm	574:631	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	7	37	theme	booting	1298:1304	arg1	stage					1306:1310	booting stage	1298:1310	booting stage	1298:1310	This study indicated that nitrogen application at booting stage significantly affected the structural characteristics of starch, and ultimately determines its quality.					
32871126	5	38	from	effect	959:964	arg1	expression					1000:1009	the mRNA expression	991:1009	the mRNA expression of enzymes related to starch biosynthesis or degradation	991:1066	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	3	39	theme	nitrogen	445:452	arg1	treatment					454:462	nitrogen treatment	445:462	nitrogen treatment	445:462	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	4	40	theme	swelling	874:881	arg1	power					883:887	the swelling power	870:887	the swelling power	870:887	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	5	41	theme	enzymes	1014:1020	arg1	expression					1000:1009	the mRNA expression	991:1009	the mRNA expression of enzymes related to starch biosynthesis or degradation	991:1066	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	7	42	theme	nitrogen	1274:1281	arg1	application					1283:1293	nitrogen application	1274:1293	nitrogen application at booting stage	1274:1310	This study indicated that nitrogen application at booting stage significantly affected the structural characteristics of starch, and ultimately determines its quality.					
32871126	2	43	theme	structural	377:386	arg1	characteristics					388:402	structural characteristics	377:402	structural characteristics	377:402	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	0	44	theme	accumulation	25:36	arg1	Analysis					0:7	Analysis	0:7	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.	0:128	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	3	45	theme	small	599:603	arg1	granule					612:618	B-type small starch granule	592:618	B-type small starch granule	592:618	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	4	46	from	lamellae	778:785	arg1	region					825:830	the external region	812:830	the external region of starch granules	812:849	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	0	47	theme	characteristics	53:67	arg1	Analysis					0:7	Analysis	0:7	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.	0:128	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	4	48	theme	granules	842:849	arg1	region					825:830	the external region	812:830	the external region of starch granules	812:849	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	3	49	theme	starch	605:610	arg1	granule					612:618	B-type small starch granule	592:618	B-type small starch granule	592:618	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	5	50	theme	nitrogen	969:976	arg1	treatment					978:986	nitrogen treatment	969:986	nitrogen treatment	969:986	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	0	51	theme	structural	42:51	arg1	characteristics					53:67	structural characteristics	42:67	structural characteristics	42:67	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	4	52	theme	starch	835:840	arg1	granules					842:849	starch granules	835:849	starch granules	835:849	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	53	theme	inner	772:776	arg1	lamellae					778:785	inner lamellae	772:785	inner lamellae	772:785	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	5	54	theme	related	1022:1028	arg1	enzymes					1014:1020	enzymes	1014:1020	enzymes related to starch biosynthesis or degradation	1014:1066	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	4	55	theme	crystal	747:753	arg1	structure					755:763	the crystal structure	743:763	the crystal structure in the inner lamellae and ordered structure in the external region of starch granules	743:849	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	1	56	contain	has	199:201	arg1	Nitrogen					130:137	Nitrogen	130:137	Nitrogen	130:137	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	1	56	contain	has	199:201	arg2	influence					214:222	a critical influence	203:222	a critical influence on yield and quality	203:243	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	1	57	theme	critical	205:212	arg1	influence					214:222	a critical influence	203:222	a critical influence on yield and quality	203:243	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	1	58	from	influence	214:222	arg1	quality					237:243	quality	237:243	quality	237:243	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	1	58	from	influence	214:222	arg1	yield					227:231	yield	227:231	yield	227:231	Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality.					
32871126	0	59	from	grain	96:100	arg1	Analysis					0:7	Analysis	0:7	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.	0:128	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	3	60	theme	B-type	592:597	arg1	granule					612:618	B-type small starch granule	592:618	B-type small starch granule	592:618	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	4	61	from	structure	755:763	arg1	structure					799:807	ordered structure	791:807	ordered structure	791:807	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	61	from	structure	755:763	arg1	lamellae					778:785	inner lamellae	772:785	inner lamellae	772:785	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	62	dep	composition	681:691	arg1	the					677:679	the	677:679	the	677:679	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	7	63	from	stage	1306:1310	arg1	application					1283:1293	nitrogen application	1274:1293	nitrogen application at booting stage	1274:1310	This study indicated that nitrogen application at booting stage significantly affected the structural characteristics of starch, and ultimately determines its quality.					
32871126	0	64	theme	starch	72:77	arg1	granule					79:85	starch granule	72:85	starch granule	72:85	Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application.					
32871126	5	65	theme	developmental	1088:1100	arg1	stages					1102:1107	different developmental stages	1078:1107	different developmental stages	1078:1107	The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages.					
32871126	3	66	theme	starch	497:502	arg1	biosynthesis					481:492	the biosynthesis	477:492	the biosynthesis of starch and amylose	477:514	The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0.					
32871126	4	67	theme	starch	907:912	arg1	solubility					893:902	solubility	893:902	solubility	893:902	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	4	67	theme	starch	907:912	arg1	power					883:887	the swelling power	870:887	the swelling power	870:887	In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased.					
32871126	2	68	theme	wheat	407:411	arg1	starch					413:418	wheat starch	407:418	wheat starch	407:418	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32871126	2	69	theme	granule	331:337	arg1	development					339:349	the starch granule development	320:349	the starch granule development	320:349	This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch.					
32119847	8	0	theme	accurate	1184:1191	arg1	measurement					1206:1216	accurate glycopeptide measurement	1184:1216	accurate glycopeptide measurement	1184:1216	We show that related glycoforms must fall in separate windows to allow accurate glycopeptide measurement.					
32119847	6	1	theme	variable	937:944	arg1	windows					952:958	variable width windows	937:958	variable width windows optimized for glycopeptides	937:986	Here, we improved the performance of DIA glycoproteomics by using variable width windows optimized for glycopeptides.					
32119847	4	2	theme	width	615:619	arg1	windows					625:631	Variable width DIA windows	606:631	Variable width DIA windows	606:631	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	2	3	theme	MS	358:359	arg1	method					361:366	a data independent acquisition (DIA) MS method	321:366	a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications	321:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	6	4	theme	DIA	908:910	arg1	glycoproteomics					912:926	DIA glycoproteomics	908:926	DIA glycoproteomics	908:926	Here, we improved the performance of DIA glycoproteomics by using variable width windows optimized for glycopeptides.					
32119847	10	5	theme	methods	1509:1515	arg1	importance					1467:1476	the importance	1463:1476	the importance of appropriately optimized DIA methods for measurement of post-translationally modified peptides	1463:1573	Our results highlight the importance of appropriately optimized DIA methods for measurement of post-translationally modified peptides.					
32119847	3	6	theme	high-sensitivity	553:568	arg1	detection					570:578	high-sensitivity detection	553:578	high-sensitivity detection	553:578	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	10	7	theme	modified	1557:1564	arg1	peptides					1566:1573	post-translationally modified peptides	1536:1573	post-translationally modified peptides	1536:1573	Our results highlight the importance of appropriately optimized DIA methods for measurement of post-translationally modified peptides.					
32119847	10	8	theme	optimized	1495:1503	arg1	methods					1509:1515	appropriately optimized DIA methods	1481:1515	appropriately optimized DIA methods	1481:1515	Our results highlight the importance of appropriately optimized DIA methods for measurement of post-translationally modified peptides.					
32119847	2	9	theme	data	323:326	arg1	method					361:366	a data independent acquisition (DIA) MS method	321:366	a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications	321:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	3	10	theme	MS	529:530	arg1	acquisition					537:547	comprehensive MS data acquisition	515:547	comprehensive MS data acquisition	515:547	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	2	11	dep	spectra	266:272	arg1	spectrometry					279:290	mass spectrometry	274:290	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	2	11	dep	spectra	266:272	arg1	SWATH					293:297	SWATH	293:297	SWATH	293:297	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	9	12	theme	wall	1282:1285	arg1	glycoproteomes					1287:1300	the cell wall glycoproteomes	1273:1300	the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast	1273:1355	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	2	13	theme	ion	262:264	arg1	spectra					266:272	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	2	14	theme	theoretical	241:251	arg1	spectra					266:272	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	1	15	theme	protein	154:160	arg1	folding					162:168	protein folding	154:168	protein folding	154:168	N-glycosylation plays an essential role in regulating protein folding and function in eukaryotic cells.					
32119847	0	16	theme	acquisition	65:75	arg1	analysis					90:97	improved data independent acquisition glycoprotein analysis	39:97	improved data independent acquisition glycoprotein analysis	39:97	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	9	17	theme	biosynthesis	1328:1339	arg1	glycoproteomes					1287:1300	the cell wall glycoproteomes	1273:1300	the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast	1273:1355	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	5	18	theme	large	846:850	arg1	structures					859:868	their large glycan structures	840:868	their large glycan structures	840:868	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	7	19	theme	narrow	1011:1016	arg1	windows					1018:1024	narrow windows	1011:1024	narrow windows	1011:1024	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
32119847	8	20	theme	glycopeptide	1193:1204	arg1	measurement					1206:1216	accurate glycopeptide measurement	1184:1216	accurate glycopeptide measurement	1184:1216	We show that related glycoforms must fall in separate windows to allow accurate glycopeptide measurement.					
32119847	4	21	theme	m/z	727:729	arg1	range					731:735	the full m/z range	718:735	the full m/z range	718:735	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	1	22	from	function	174:181	arg1	cells					197:201	eukaryotic cells	186:201	eukaryotic cells	186:201	N-glycosylation plays an essential role in regulating protein folding and function in eukaryotic cells.					
32119847	5	23	theme	m/z	751:753	arg1	distribution					755:766	the m/z distribution	747:766	the m/z distribution of glycopeptides	747:783	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	5	23	theme	m/z	751:753	arg1	different					788:796	different	788:796	different	788:796	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	1	24	from	folding	162:168	arg1	cells					197:201	eukaryotic cells	186:201	eukaryotic cells	186:201	N-glycosylation plays an essential role in regulating protein folding and function in eukaryotic cells.					
32119847	0	25	gly	glycoprotein	77:88	arg1	glycoprotein					77:88	improved data independent acquisition glycoprotein analysis	39:97	improved data independent acquisition glycoprotein analysis	39:97	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	3	26	theme	entire	448:453	arg1	range					459:463	the entire m/z range	444:463	the entire m/z range	444:463	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	4	27	theme	DIA	621:623	arg1	windows					625:631	Variable width DIA windows	606:631	Variable width DIA windows	606:631	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	10	28	theme	peptides	1566:1573	arg1	measurement					1521:1531	measurement	1521:1531	measurement of post-translationally modified peptides	1521:1573	Our results highlight the importance of appropriately optimized DIA methods for measurement of post-translationally modified peptides.					
32119847	0	29	theme	glycoprotein	77:88	arg1	analysis					90:97	improved data independent acquisition glycoprotein analysis	39:97	improved data independent acquisition glycoprotein analysis	39:97	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	2	30	theme	glycoproteins	384:396	arg1	analysis					372:379	analysis	372:379	analysis of glycoproteins and their glycan modifications	372:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	9	31	theme	glycopeptides	1393:1405	arg1	measurement					1378:1388	improved measurement	1369:1388	improved measurement of glycopeptides with different glycan structures	1369:1438	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	3	32	theme	consecutive	470:480	arg1	windows					492:498	consecutive isolation windows	470:498	consecutive isolation windows	470:498	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	0	33	theme	window	22:27	arg1	SWATH					29:33	variable window SWATH	13:33	variable window SWATH	13:33	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	9	34	theme	different	1412:1420	arg1	structures					1429:1438	different glycan structures	1412:1438	different glycan structures	1412:1438	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	1	35	theme	essential	125:133	arg1	role					135:138	an essential role	122:138	an essential role	122:138	N-glycosylation plays an essential role in regulating protein folding and function in eukaryotic cells.					
32119847	2	36	theme	modifications	415:427	arg1	analysis					372:379	analysis	372:379	analysis of glycoproteins and their glycan modifications	372:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	8	37	theme	related	1126:1132	arg1	glycoforms					1134:1143	related glycoforms	1126:1143	related glycoforms	1126:1143	We show that related glycoforms must fall in separate windows to allow accurate glycopeptide measurement.					
32119847	0	38	theme	data	48:51	arg1	analysis					90:97	improved data independent acquisition glycoprotein analysis	39:97	improved data independent acquisition glycoprotein analysis	39:97	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	2	39	theme	mass	274:277	arg1	spectrometry					279:290	mass spectrometry	274:290	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	2	39	theme	mass	274:277	arg1	SWATH					293:297	SWATH	293:297	SWATH	293:297	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	2	40	theme	window	215:220	arg1	acquisition					222:232	Sequential window acquisition	204:232	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH)	204:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	3	41	theme	data	532:535	arg1	acquisition					537:547	comprehensive MS data acquisition	515:547	comprehensive MS data acquisition	515:547	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	0	42	theme	independent	53:63	arg1	analysis					90:97	improved data independent acquisition glycoprotein analysis	39:97	improved data independent acquisition glycoprotein analysis	39:97	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	6	43	theme	width	946:950	arg1	windows					952:958	variable width windows	937:958	variable width windows optimized for glycopeptides	937:986	Here, we improved the performance of DIA glycoproteomics by using variable width windows optimized for glycopeptides.					
32119847	4	44	theme	analyte	647:653	arg1	measurement					655:665	optimal analyte measurement	639:665	optimal analyte measurement	639:665	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	3	45	theme	comprehensive	515:527	arg1	acquisition					537:547	comprehensive MS data acquisition	515:547	comprehensive MS data acquisition	515:547	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	4	46	theme	Variable	606:613	arg1	windows					625:631	Variable width DIA windows	606:631	Variable width DIA windows	606:631	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	10	47	theme	DIA	1505:1507	arg1	methods					1509:1515	appropriately optimized DIA methods	1481:1515	appropriately optimized DIA methods	1481:1515	Our results highlight the importance of appropriately optimized DIA methods for measurement of post-translationally modified peptides.					
32119847	6	48	gly	glycopeptides	974:986	arg2	glycopeptides					974:986	glycopeptides	974:986	glycopeptides	974:986	Here, we improved the performance of DIA glycoproteomics by using variable width windows optimized for glycopeptides.					
32119847	2	49	theme	independent	328:338	arg1	method					361:366	a data independent acquisition (DIA) MS method	321:366	a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications	321:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	6	50	theme	glycoproteomics	912:926	arg1	performance					893:903	the performance	889:903	the performance of DIA glycoproteomics	889:926	Here, we improved the performance of DIA glycoproteomics by using variable width windows optimized for glycopeptides.					
32119847	9	51	dep	wild-type	1305:1313	arg1	yeast					1351:1355	deficient yeast	1341:1355	deficient yeast	1341:1355	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	1	52	theme	eukaryotic	186:195	arg1	cells					197:201	eukaryotic cells	186:201	eukaryotic cells	186:201	N-glycosylation plays an essential role in regulating protein folding and function in eukaryotic cells.					
32119847	9	53	theme	method	1253:1258	arg1	utility					1238:1244	the utility	1234:1244	the utility of the method	1234:1258	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	3	54	theme	interest	596:603	arg1	molecules					583:591	molecules	583:591	molecules of interest	583:603	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	3	55	theme	molecules	583:591	arg1	detection					570:578	high-sensitivity detection	553:578	high-sensitivity detection	553:578	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	3	55	theme	molecules	583:591	arg1	acquisition					537:547	comprehensive MS data acquisition	515:547	comprehensive MS data acquisition	515:547	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	5	56	theme	glycan	852:857	arg1	structures					859:868	their large glycan structures	840:868	their large glycan structures	840:868	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	7	57	from	rich	1040:1043	arg1	glycopeptides					1048:1060	glycopeptides	1048:1060	glycopeptides	1048:1060	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
32119847	9	58	theme	cell	1277:1280	arg1	glycoproteomes					1287:1300	the cell wall glycoproteomes	1273:1300	the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast	1273:1355	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	2	59	theme	spectra	266:272	arg1	acquisition					222:232	Sequential window acquisition	204:232	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH)	204:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	4	60	theme	peptide	671:677	arg1	ions					679:682	peptide ions	671:682	peptide ions	671:682	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	7	61	theme	analytical	1073:1082	arg1	specificity					1084:1094	analytical specificity	1073:1094	analytical specificity	1073:1094	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
32119847	2	62	theme	fragment	253:260	arg1	spectra					266:272	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	all theoretical fragment ion spectra mass spectrometry (SWATH)	237:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	9	63	with	measurement	1378:1388	arg1	structures					1429:1438	different glycan structures	1412:1438	different glycan structures	1412:1438	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	9	64	theme	wild-type	1305:1313	arg1	glycoproteomes					1287:1300	the cell wall glycoproteomes	1273:1300	the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast	1273:1355	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	7	65	theme	rich	1040:1043	arg1	ranges					1033:1038	m/z ranges	1029:1038	m/z ranges rich in glycopeptides	1029:1060	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
32119847	5	66	theme	glycopeptides	771:783	arg1	distribution					755:766	the m/z distribution	747:766	the m/z distribution of glycopeptides	747:783	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	5	66	theme	glycopeptides	771:783	arg1	different					788:796	different	788:796	different	788:796	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	9	67	theme	N-glycan	1319:1326	arg1	biosynthesis					1328:1339	N-glycan biosynthesis	1319:1339	N-glycan biosynthesis	1319:1339	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	7	68	theme	m/z	1029:1031	arg1	ranges					1033:1038	m/z ranges	1029:1038	m/z ranges rich in glycopeptides	1029:1060	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
32119847	9	69	theme	deficient	1341:1349	arg1	yeast					1351:1355	deficient yeast	1341:1355	deficient yeast	1341:1355	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	4	70	theme	full	722:725	arg1	range					731:735	the full m/z range	718:735	the full m/z range	718:735	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	9	71	gly	glycopeptides	1393:1405	arg2	glycopeptides					1393:1405	glycopeptides	1393:1405	glycopeptides	1393:1405	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	5	72	gly	glycopeptides	771:783	arg2	glycopeptides					771:783	glycopeptides	771:783	glycopeptides	771:783	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	9	73	theme	improved	1369:1376	arg1	measurement					1378:1388	improved measurement	1369:1388	improved measurement of glycopeptides with different glycan structures	1369:1438	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	3	74	theme	m/z	455:457	arg1	range					459:463	the entire m/z range	444:463	the entire m/z range	444:463	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	0	75	theme	variable	13:20	arg1	SWATH					29:33	variable window SWATH	13:33	variable window SWATH	13:33	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	2	76	gly	glycoproteins	384:396	arg1	glycoproteins					384:396	glycoproteins	384:396	glycoproteins	384:396	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	2	77	theme	Sequential	204:213	arg1	acquisition					222:232	Sequential window acquisition	204:232	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH)	204:298	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	3	78	theme	isolation	482:490	arg1	windows					492:498	consecutive isolation windows	470:498	consecutive isolation windows	470:498	By separating the entire m/z range into consecutive isolation windows, DIA-MS allows comprehensive MS data acquisition and high-sensitivity detection of molecules of interest.					
32119847	0	79	theme	improved	39:46	arg1	analysis					90:97	improved data independent acquisition glycoprotein analysis	39:97	improved data independent acquisition glycoprotein analysis	39:97	Glycopeptide variable window SWATH for improved data independent acquisition glycoprotein analysis.					
32119847	9	80	theme	glycan	1422:1427	arg1	structures					1429:1438	different glycan structures	1412:1438	different glycan structures	1412:1438	We demonstrate the utility of the method by comparing the cell wall glycoproteomes of wild-type and N-glycan biosynthesis deficient yeast and showing improved measurement of glycopeptides with different glycan structures.					
32119847	7	81	gly	glycopeptides	1048:1060	arg2	glycopeptides					1048:1060	glycopeptides	1048:1060	glycopeptides	1048:1060	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
32119847	2	82	theme	glycan	408:413	arg1	modifications					415:427	their glycan modifications	402:427	their glycan modifications	402:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	5	83	theme	unmodified	809:818	arg1	peptides					820:827	unmodified peptides	809:827	unmodified peptides	809:827	However, the m/z distribution of glycopeptides is different to that of unmodified peptides because of their large glycan structures.					
32119847	2	84	theme	DIA	353:355	arg1	method					361:366	a data independent acquisition (DIA) MS method	321:366	a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications	321:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	8	85	theme	separate	1158:1165	arg1	windows					1167:1173	separate windows	1158:1173	separate windows	1158:1173	We show that related glycoforms must fall in separate windows to allow accurate glycopeptide measurement.					
32119847	4	86	theme	optimal	639:645	arg1	measurement					655:665	optimal analyte measurement	639:665	optimal analyte measurement	639:665	Variable width DIA windows allow optimal analyte measurement, as peptide ions are not evenly distributed across the full m/z range.					
32119847	8	87	gly	glycopeptide	1193:1204	arg2	glycopeptide					1193:1204	accurate glycopeptide measurement	1184:1216	accurate glycopeptide measurement	1184:1216	We show that related glycoforms must fall in separate windows to allow accurate glycopeptide measurement.					
32119847	2	88	theme	acquisition	340:350	arg1	method					361:366	a data independent acquisition (DIA) MS method	321:366	a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications	321:427	Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH) has proven useful as a data independent acquisition (DIA) MS method for analysis of glycoproteins and their glycan modifications.					
32119847	7	89	from	glycopeptides	1048:1060	arg1	rich					1040:1043	rich	1040:1043	rich	1040:1043	This method allocates narrow windows at m/z ranges rich in glycopeptides, improving analytical specificity and performance.					
34651345	1	0	dep	-ibuprofen	597:606	arg1	S					595:595	including (R,S)-ibuprofen	582:606	S	595:595	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	1	from	determination	468:480	arg1	formulations					568:579	the commercial pharmaceutical formulations	538:579	the commercial pharmaceutical formulations	538:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	2	2	theme	additives	804:812	arg1	temperature					826:836	column temperature	819:836	column temperature	819:836	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	2	theme	additives	804:812	arg1	phase					734:738	mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature	727:836	phase	734:738	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	2	theme	additives	804:812	arg1	proportion					753:762	proportion	753:762	proportion of organic modifier	753:782	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	2	theme	additives	804:812	arg1	percentage					785:794	percentage	785:794	percentage of acid additives	785:812	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	3	3	theme	enantiomeric	1131:1142	arg1	resolutions					1144:1154	the excellent enantiomeric resolutions	1117:1154	the excellent enantiomeric resolutions	1117:1154	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	3	4	theme	%	996:996	arg1	acid					1005:1008	0.1% formic acid	993:1008	0.1% formic acid	993:1008	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	3	4	theme	%	996:996	arg1	acetonitrile-water					973:990	acetonitrile-water	973:990	acetonitrile-water (0.1% formic acid, v/v)	973:1014	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	7	5	theme	enantiomers	1777:1787	arg1	determination					1754:1766	determination	1754:1766	determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers	1754:1870	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	4	6	theme	developed	1262:1270	arg1	method					1272:1277	The newly developed method	1252:1277	The newly developed method	1252:1277	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	7	7	from	determination	1754:1766	arg1	formulations					1807:1818	pharmaceutical formulations	1792:1818	pharmaceutical formulations containing racemic mixtures or single stereoisomers	1792:1870	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	2	8	theme	mobile	727:732	arg1	phase					734:738	mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature	727:836	phase	734:738	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	0	9	theme	racemic	236:242	arg1	mixtures					244:251	racemic mixtures	236:251	racemic mixtures	236:251	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	10	with	HPLC	140:143	arg1	focus					152:156	a focus	150:156	a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	150:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	3	11	theme	selected	1164:1171	arg1	NSAIDs					1173:1178	all selected NSAIDs	1160:1178	all selected NSAIDs	1160:1178	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	3	12	theme	enantioseparation	1196:1212	arg1	process					1214:1220	the enantioseparation process	1192:1220	the enantioseparation process	1192:1220	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	2	13	theme	proportion	753:762	arg1	influence					714:722	the influence	710:722	the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation	710:857	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	14	from	Experiments	695:705	arg1	influence					714:722	the influence	710:722	the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation	710:857	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	4	15	theme	racemic	1377:1383	arg1	mixtures					1385:1392	racemic mixtures	1377:1392	racemic mixtures	1377:1392	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	8	16	theme	commercial	1901:1910	arg1	capsules					1912:1919	the commercial capsules	1897:1919	the commercial capsules	1897:1919	Quantitative results of the commercial capsules and tablets demonstrated that the difference between the declared and measured values did not exceed 1.52%.					
34651345	1	17	theme	stationary	408:417	arg1	phase					419:423	stationary phase	408:423	stationary phase	408:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	6	18	theme	RSD	1670:1672	arg1	%					1683:1683	RSD 0.13-1.09%	1670:1683	RSD 0.13-1.09%	1670:1683	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	6	18	theme	RSD	1670:1672	arg1	precision					1659:1667	inter-day precision	1649:1667	inter-day precision (RSD 0.13-1.09%)	1649:1684	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	4	19	theme	NSAID	1317:1321	arg1	enantiomers					1323:1333	NSAID enantiomers	1317:1333	NSAID enantiomers	1317:1333	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	7	20	theme	developed	1716:1724	arg1	method					1726:1731	The newly developed method	1706:1731	The newly developed method	1706:1731	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	21	theme	enantiomeric	161:172	arg1	control					182:188	enantiomeric quality control	161:188	enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	161:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	22	theme	tris	81:84	arg1	phase					131:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase	73:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	73:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	4	23	from	determination	1300:1312	arg1	formulations					1353:1364	pharmaceutical formulations	1338:1364	pharmaceutical formulations containing racemic mixtures or single stereoisomers	1338:1416	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	24	contain	containing	225:234	arg1	formulations					212:223	six pharmaceutical formulations	193:223	six pharmaceutical formulations containing racemic mixtures or single stereoisomers	193:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	24	contain	containing	225:234	arg2	stereoisomers					263:275	single stereoisomers	256:275	single stereoisomers	256:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	24	contain	containing	225:234	arg2	mixtures					244:251	racemic mixtures	236:251	racemic mixtures	236:251	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	25	theme	3-chloro-5-methylphenylcarbamate	86:117	arg1	phase					131:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase	73:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	73:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	26	theme	amylose	358:364	arg1	3-chloro-5-methylphenylcarbamate					371:402	3-chloro-5-methylphenylcarbamate	371:402	3-chloro-5-methylphenylcarbamate	371:402	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	26	theme	amylose	358:364	arg1	tris					366:369	amylose tris	358:369	amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	358:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	27	with	proportion	1023:1032	arg1	rate					1061:1064	a flow rate	1054:1064	a flow rate of 0.6 ml/min at 22°C	1054:1086	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	1	28	with	method	346:351	arg1	3-chloro-5-methylphenylcarbamate					371:402	3-chloro-5-methylphenylcarbamate	371:402	3-chloro-5-methylphenylcarbamate	371:402	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	28	with	method	346:351	arg1	tris					366:369	amylose tris	358:369	amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	358:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	7	29	contain	containing	1820:1829	arg2	mixtures					1839:1846	racemic mixtures	1831:1846	racemic mixtures	1831:1846	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	7	29	contain	containing	1820:1829	arg1	formulations					1807:1818	pharmaceutical formulations	1792:1818	pharmaceutical formulations containing racemic mixtures or single stereoisomers	1792:1870	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	7	29	contain	containing	1820:1829	arg2	stereoisomers					1858:1870	single stereoisomers	1851:1870	single stereoisomers	1851:1870	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	30	theme	stationary	120:129	arg1	phase					131:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase	73:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	73:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	6	31	theme	RSD	1628:1630	arg1	precision					1617:1625	good intra-day precision	1602:1625	good intra-day precision (RSD 0.52-1.46%)	1602:1642	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	6	31	theme	RSD	1628:1630	arg1	%					1641:1641	RSD 0.52-1.46%	1628:1641	RSD 0.52-1.46%	1628:1641	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	1	32	theme	R	593:593	arg1	-ibuprofen					597:606	including (R,S)-ibuprofen	582:606	-ibuprofen	597:606	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	4	33	contain	containing	1366:1375	arg1	formulations					1353:1364	pharmaceutical formulations	1338:1364	pharmaceutical formulations containing racemic mixtures or single stereoisomers	1338:1416	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	4	33	contain	containing	1366:1375	arg2	stereoisomers					1404:1416	single stereoisomers	1397:1416	single stereoisomers	1397:1416	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	4	33	contain	containing	1366:1375	arg2	mixtures					1385:1392	racemic mixtures	1377:1392	racemic mixtures	1377:1392	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	34	theme	Enantioselective	0:15	arg1	separation					17:26	Enantioselective separation	0:26	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.	0:276	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	35	theme	accurate	303:310	arg1	method					346:351	an accurate, rapid, and simple chiral HPLC-UV method	300:351	an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	300:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	36	theme	50:50	1037:1041	arg1	proportion					1023:1032	the proportion	1019:1032	the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C	1019:1086	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	6	37	theme	good	1602:1605	arg1	precision					1617:1625	good intra-day precision	1602:1625	good intra-day precision (RSD 0.52-1.46%)	1602:1642	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	6	37	theme	good	1602:1605	arg1	%					1641:1641	RSD 0.52-1.46%	1628:1641	RSD 0.52-1.46%	1628:1641	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	2	38	theme	best	888:891	arg1	condition					904:912	the best separation condition	884:912	the best separation condition	884:912	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	1	39	theme	rapid	313:317	arg1	method					346:351	an accurate, rapid, and simple chiral HPLC-UV method	300:351	an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	300:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	40	theme	flow	1056:1059	arg1	rate					1061:1064	a flow rate	1054:1064	a flow rate of 0.6 ml/min at 22°C	1054:1086	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	0	41	theme	anti-inflammatory	44:60	arg1	drugs					62:66	nonsteroidal anti-inflammatory drugs	31:66	nonsteroidal anti-inflammatory drugs	31:66	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	7	42	theme	racemic	1831:1837	arg1	mixtures					1839:1846	racemic mixtures	1831:1846	racemic mixtures	1831:1846	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	43	theme	chiral	331:336	arg1	method					346:351	an accurate, rapid, and simple chiral HPLC-UV method	300:351	an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	300:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	44	from	22°C	1083:1086	arg1	rate					1061:1064	a flow rate	1054:1064	a flow rate of 0.6 ml/min at 22°C	1054:1086	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	8	45	theme	Quantitative	1873:1884	arg1	results					1886:1892	Quantitative results	1873:1892	Quantitative results of the commercial capsules and tablets	1873:1931	Quantitative results of the commercial capsules and tablets demonstrated that the difference between the declared and measured values did not exceed 1.52%.					
34651345	2	46	theme	percentage	785:794	arg1	influence					714:722	the influence	710:722	the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation	710:857	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	1	47	theme	anti-inflammatory	502:518	arg1	drugs					520:524	six nonsteroidal anti-inflammatory drugs	485:524	six nonsteroidal anti-inflammatory drugs (NSAIDs)	485:533	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	47	theme	anti-inflammatory	502:518	arg1	NSAIDs					527:532	NSAIDs	527:532	NSAIDs	527:532	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	48	theme	commercial	542:551	arg1	formulations					568:579	the commercial pharmaceutical formulations	538:579	the commercial pharmaceutical formulations	538:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	7	49	theme	single	1851:1856	arg1	stereoisomers					1858:1870	single stereoisomers	1851:1870	single stereoisomers	1851:1870	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	2	50	theme	modifier	775:782	arg1	temperature					826:836	column temperature	819:836	column temperature	819:836	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	50	theme	modifier	775:782	arg1	phase					734:738	mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature	727:836	phase	734:738	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	50	theme	modifier	775:782	arg1	proportion					753:762	proportion	753:762	proportion of organic modifier	753:782	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	50	theme	modifier	775:782	arg1	percentage					785:794	percentage	785:794	percentage of acid additives	785:812	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	51	theme	temperature	826:836	arg1	influence					714:722	the influence	710:722	the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation	710:857	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	52	dep	phase	734:738	arg1	composition					740:750	composition	740:750	composition	740:750	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	1	53	theme	-loxoprofen	675:685	arg1	sodium					687:692	(R,S)-loxoprofen sodium	670:692	sodium	687:692	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	0	54	theme	pharmaceutical	197:210	arg1	formulations					212:223	six pharmaceutical formulations	193:223	six pharmaceutical formulations containing racemic mixtures or single stereoisomers	193:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	55	theme	including	582:590	arg1	-ibuprofen					597:606	including (R,S)-ibuprofen	582:606	-ibuprofen	597:606	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	56	dep	acid	1005:1008	arg1	v/v					1011:1013	v/v	1011:1013	v/v	1011:1013	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	1	57	theme	present	285:291	arg1	study					293:297	the present study	281:297	the present study	281:297	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	58	theme	0.1	993:995	arg1	%					996:996	%	996:996	%	996:996	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	2	59	theme	acid	799:802	arg1	additives					804:812	acid additives	799:812	acid additives	799:812	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	0	60	from	control	182:188	arg1	formulations					212:223	six pharmaceutical formulations	193:223	six pharmaceutical formulations containing racemic mixtures or single stereoisomers	193:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	5	61	theme	Calibration	1419:1429	arg1	curve					1431:1435	Calibration curve	1419:1435	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml	1419:1492	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml showed good linearity with the correlation coefficient above 0.9996.					
34651345	2	62	theme	phase	734:738	arg1	influence					714:722	the influence	710:722	the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation	710:857	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	1	63	theme	nonsteroidal	489:500	arg1	drugs					520:524	six nonsteroidal anti-inflammatory drugs	485:524	six nonsteroidal anti-inflammatory drugs (NSAIDs)	485:533	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	63	theme	nonsteroidal	489:500	arg1	NSAIDs					527:532	NSAIDs	527:532	NSAIDs	527:532	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	64	theme	excellent	1121:1129	arg1	resolutions					1144:1154	the excellent enantiomeric resolutions	1117:1154	the excellent enantiomeric resolutions	1117:1154	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	1	65	dep	-ketoprofen	627:637	arg1	R					623:623	R	623:623	R	623:623	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	65	dep	-ketoprofen	627:637	arg1	S					625:625	S	625:625	S	625:625	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	0	66	theme	single	256:261	arg1	stereoisomers					263:275	single stereoisomers	256:275	single stereoisomers	256:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	67	theme	drugs	520:524	arg1	-ibuprofen					597:606	including (R,S)-ibuprofen	582:606	-ibuprofen	597:606	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	67	theme	drugs	520:524	arg1	determination					468:480	enantiomeric determination	455:480	enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations	455:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	67	theme	drugs	520:524	arg1	sodium					687:692	(R,S)-loxoprofen sodium	670:692	sodium	687:692	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	68	theme	formic	998:1003	arg1	acid					1005:1008	0.1% formic acid	993:1008	0.1% formic acid	993:1008	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	3	68	theme	formic	998:1003	arg1	acetonitrile-water					973:990	acetonitrile-water	973:990	acetonitrile-water (0.1% formic acid, v/v)	973:1014	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	4	69	theme	pharmaceutical	1338:1351	arg1	formulations					1353:1364	pharmaceutical formulations	1338:1364	pharmaceutical formulations containing racemic mixtures or single stereoisomers	1338:1416	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	8	70	theme	capsules	1912:1919	arg1	results					1886:1892	Quantitative results	1873:1892	Quantitative results of the commercial capsules and tablets	1873:1931	Quantitative results of the commercial capsules and tablets demonstrated that the difference between the declared and measured values did not exceed 1.52%.					
34651345	8	71	theme	tablets	1925:1931	arg1	results					1886:1892	Quantitative results	1873:1892	Quantitative results of the commercial capsules and tablets	1873:1931	Quantitative results of the commercial capsules and tablets demonstrated that the difference between the declared and measured values did not exceed 1.52%.					
34651345	1	72	theme	R	671:671	arg1	sodium					687:692	(R,S)-loxoprofen sodium	670:692	sodium	687:692	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	0	73	theme	quality	174:180	arg1	control					182:188	enantiomeric quality control	161:188	enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	161:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	5	74	from	concentration	1463:1475	arg1	curve					1431:1435	Calibration curve	1419:1435	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml	1419:1492	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml showed good linearity with the correlation coefficient above 0.9996.					
34651345	6	75	theme	inter-day	1649:1657	arg1	%					1683:1683	RSD 0.13-1.09%	1670:1683	RSD 0.13-1.09%	1670:1683	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	6	75	theme	inter-day	1649:1657	arg1	precision					1659:1667	inter-day precision	1649:1667	inter-day precision (RSD 0.13-1.09%)	1649:1684	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	1	76	from	-ibuprofen	597:606	arg1	formulations					568:579	the commercial pharmaceutical formulations	538:579	the commercial pharmaceutical formulations	538:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	4	77	theme	enantiomers	1323:1333	arg1	determination					1300:1312	determination	1300:1312	determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers	1300:1416	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	0	78	from	phase	131:135	arg1	HPLC					140:143	HPLC	140:143	HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	140:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	79	theme	enantiomeric	455:466	arg1	determination					468:480	enantiomeric determination	455:480	enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations	455:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	6	80	theme	Satisfactory	1563:1574	arg1	recovery					1576:1583	Satisfactory recovery	1563:1583	Satisfactory recovery (96.54-101.54%)	1563:1599	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	6	80	theme	Satisfactory	1563:1574	arg1	%					1598:1598	96.54-101.54%	1586:1598	96.54-101.54%	1586:1598	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	8	81	theme	measured	1991:1998	arg1	values					2000:2005	measured values	1991:2005	measured values	1991:2005	Quantitative results of the commercial capsules and tablets demonstrated that the difference between the declared and measured values did not exceed 1.52%.					
34651345	1	82	dep	S-naproxen	654:663	arg1	S-ketoprofen					640:651	S-ketoprofen	640:651	S-ketoprofen	640:651	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	5	83	theme	ug/ml	1488:1492	arg1	concentration					1463:1475	the concentration	1459:1475	the concentration of 5.0-100 ug/ml	1459:1492	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml showed good linearity with the correlation coefficient above 0.9996.					
34651345	1	84	dep	sodium	687:692	arg1	S					673:673	(R,S)-loxoprofen sodium	670:692	S	673:673	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	4	85	theme	single	1397:1402	arg1	stereoisomers					1404:1416	single stereoisomers	1397:1416	single stereoisomers	1397:1416	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	5	86	theme	enantiomer	1445:1454	arg1	curve					1431:1435	Calibration curve	1419:1435	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml	1419:1492	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml showed good linearity with the correlation coefficient above 0.9996.					
34651345	0	87	from	focus	152:156	arg1	control					182:188	enantiomeric quality control	161:188	enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	161:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	6	88	theme	intra-day	1607:1615	arg1	precision					1617:1625	good intra-day precision	1602:1625	good intra-day precision (RSD 0.52-1.46%)	1602:1642	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	6	88	theme	intra-day	1607:1615	arg1	%					1641:1641	RSD 0.52-1.46%	1628:1641	RSD 0.52-1.46%	1628:1641	Satisfactory recovery (96.54-101.54%), good intra-day precision (RSD 0.52-1.46%), and inter-day precision (RSD 0.13-1.09%) were also obtained.					
34651345	2	89	theme	separation	893:902	arg1	condition					904:912	the best separation condition	884:912	the best separation condition	884:912	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	0	90	theme	nonsteroidal	31:42	arg1	drugs					62:66	nonsteroidal anti-inflammatory drugs	31:66	nonsteroidal anti-inflammatory drugs	31:66	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	5	91	theme	correlation	1525:1535	arg1	coefficient					1537:1547	the correlation coefficient	1521:1547	the correlation coefficient above 0.9996	1521:1560	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml showed good linearity with the correlation coefficient above 0.9996.					
34651345	0	92	theme	drugs	62:66	arg1	separation					17:26	Enantioselective separation	0:26	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.	0:276	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	2	93	from	influence	714:722	arg1	enantioseparation					841:857	enantioseparation	841:857	enantioseparation	841:857	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	0	94	theme	amylose	73:79	arg1	phase					131:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase	73:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	73:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	95	theme	simple	324:329	arg1	method					346:351	an accurate, rapid, and simple chiral HPLC-UV method	300:351	an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	300:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	3	96	theme	0.6 ml/min	1069:1078	arg1	rate					1061:1064	a flow rate	1054:1064	a flow rate of 0.6 ml/min at 22°C	1054:1086	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	3	97	theme	mobile	941:946	arg1	phase					948:952	one mobile phase	937:952	one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C	937:1086	It was indicated that one mobile phase simply composed of acetonitrile-water (0.1% formic acid, v/v) at the proportion of 50:50 (v/v) with a flow rate of 0.6 ml/min at 22°C could simultaneously provide the excellent enantiomeric resolutions for all selected NSAIDs, which made the enantioseparation process more applicable and operable.					
34651345	5	98	theme	good	1501:1504	arg1	linearity					1506:1514	good linearity	1501:1514	good linearity	1501:1514	Calibration curve of each enantiomer at the concentration of 5.0-100 ug/ml showed good linearity with the correlation coefficient above 0.9996.					
34651345	0	99	with	separation	17:26	arg1	phase					131:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase	73:135	amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers	73:275	Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	100	theme	HPLC-UV	338:344	arg1	method					346:351	an accurate, rapid, and simple chiral HPLC-UV method	300:351	an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase	300:423	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	1	101	from	sodium	687:692	arg1	formulations					568:579	the commercial pharmaceutical formulations	538:579	the commercial pharmaceutical formulations	538:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	7	102	theme	NSAID	1771:1775	arg1	enantiomers					1777:1787	NSAID enantiomers	1771:1787	NSAID enantiomers	1771:1787	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	1	103	theme	pharmaceutical	553:566	arg1	formulations					568:579	the commercial pharmaceutical formulations	538:579	the commercial pharmaceutical formulations	538:579	In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium.					
34651345	7	104	theme	pharmaceutical	1792:1805	arg1	formulations					1807:1818	pharmaceutical formulations	1792:1818	pharmaceutical formulations containing racemic mixtures or single stereoisomers	1792:1870	The newly developed method was then applied for determination of NSAID enantiomers in pharmaceutical formulations containing racemic mixtures or single stereoisomers.					
34651345	2	105	theme	organic	767:773	arg1	modifier					775:782	organic modifier	767:782	organic modifier	767:782	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
34651345	2	106	theme	column	819:824	arg1	temperature					826:836	column temperature	819:836	column temperature	819:836	Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition.					
33483022	3	0	with	CNCs	593:596	arg1	morphologies					613:624	different morphologies	603:624	different morphologies	603:624	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	3	1	theme	films	681:685	arg1	transparency					651:662	the transparency	647:662	the transparency of the composite films	647:685	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	4	2	theme	composite	854:862	arg1	films					864:868	the composite films	850:868	the composite films	850:868	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	2	3	theme	nanocrystallines	443:458	arg1	effects					411:417	the effects	407:417	the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films	407:542	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	2	4	theme	nanocrystallines	492:507	arg1	effects					411:417	the effects	407:417	the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films	407:542	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	4	5	dep	better	799:804	arg1	a					797:797	a	797:797	a	797:797	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	6	from	°C.	911:913	arg1	contrary					922:929	the contrary	918:929	the contrary	918:929	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	2	7	theme	cellulose	482:490	arg1	SCNCs					510:514	SCNCs	510:514	SCNCs	510:514	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	2	7	theme	cellulose	482:490	arg1	nanocrystallines					492:507	spherical cellulose nanocrystallines	472:507	spherical cellulose nanocrystallines (SCNCs)	472:515	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	4	8	theme	thermal	829:835	arg1	stability					837:845	the thermal stability	825:845	the thermal stability of the composite films	825:868	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	9	theme	mechanical	944:953	arg1	higher					1008:1013	higher	1008:1013	higher	1008:1013	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	9	theme	mechanical	944:953	arg1	properties					955:964	the maximum mechanical properties	932:964	the maximum mechanical properties of the composite films of RCNCs	932:996	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	1	10	theme	flexible	259:266	arg1	films					298:302	flexible and transparent nanocomposite films	259:302	flexible and transparent nanocomposite films with PVA matrix	259:318	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	0	11	theme	polyvinyl	108:116	arg1	alcohol					118:124	polyvinyl alcohol	108:124	polyvinyl alcohol	108:124	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	0	12	from	enzymolysis	50:60	arg1	nanocrystals					32:43	Spherical vs rod-like cellulose nanocrystals	0:43	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.	0:125	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	3	13	dep	additive	745:752	arg1	amount					754:759	amount	754:759	amount	754:759	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	4	14	theme	composite	973:981	arg1	films					983:987	the composite films	969:987	the composite films of RCNCs	969:996	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	15	theme	PVA/RCNCs	1067:1075	arg1	film					1087:1090	the PVA/RCNCs composite film	1063:1090	the PVA/RCNCs composite film	1063:1090	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	1	16	theme	cellulose	159:167	arg1	nanocrystallines					169:184	cellulose nanocrystallines	159:184	cellulose nanocrystallines (CNCs) with different morphologies	159:219	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	1	16	theme	cellulose	159:167	arg1	CNCs					187:190	CNCs	187:190	CNCs	187:190	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	4	17	theme	films	983:987	arg1	higher					1008:1013	higher	1008:1013	higher	1008:1013	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	17	theme	films	983:987	arg1	properties					955:964	the maximum mechanical properties	932:964	the maximum mechanical properties of the composite films of RCNCs	932:996	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	1	18	with	films	298:302	arg1	matrix					313:318	PVA matrix	309:318	PVA matrix	309:318	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	2	19	from	effects	411:417	arg1	films					538:542	PVA nanocomposite films	520:542	PVA nanocomposite films	520:542	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	0	20	theme	Spherical	0:8	arg1	nanocrystals					32:43	Spherical vs rod-like cellulose nanocrystals	0:43	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.	0:125	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	4	21	theme	pure	1142:1145	arg1	PVA					1147:1149	the pure PVA	1138:1149	the pure PVA	1138:1149	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	3	22	theme	little	630:635	arg1	effect					637:642	little effect	630:642	little effect	630:642	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	0	23	theme	cellulose	22:30	arg1	nanocrystals					32:43	Spherical vs rod-like cellulose nanocrystals	0:43	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.	0:125	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	1	24	theme	transparent	272:282	arg1	films					298:302	flexible and transparent nanocomposite films	259:302	flexible and transparent nanocomposite films with PVA matrix	259:318	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	4	25	theme	maximum	936:942	arg1	higher					1008:1013	higher	1008:1013	higher	1008:1013	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	25	theme	maximum	936:942	arg1	properties					955:964	the maximum mechanical properties	932:964	the maximum mechanical properties of the composite films of RCNCs	932:996	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	0	26	theme	rod-like	13:20	arg1	nanocrystals					32:43	Spherical vs rod-like cellulose nanocrystals	0:43	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.	0:125	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	1	27	theme	nanocomposite	284:296	arg1	films					298:302	flexible and transparent nanocomposite films	259:302	flexible and transparent nanocomposite films with PVA matrix	259:318	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	3	28	dep	showed	586:591	arg1	had					626:628	had	626:628	showed CNCs with different morphologies had little effect on the transparency of the composite films	586:685	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	2	29	theme	cellulose	433:441	arg1	RCNCs					461:465	RCNCs	461:465	RCNCs	461:465	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	2	29	theme	cellulose	433:441	arg1	nanocrystallines					443:458	rod-shaped cellulose nanocrystallines	422:458	rod-shaped cellulose nanocrystallines (RCNCs)	422:466	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	4	30	theme	temperature	893:903	arg1	°C.					911:913	pyrolysis temperature 60-80 °C.	883:913	pyrolysis temperature 60-80 °C. On the contrary	883:929	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	0	31	from	study	77:81	arg1	alcohol					118:124	polyvinyl alcohol	108:124	polyvinyl alcohol	108:124	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	4	32	theme	Young	1044:1048	arg1	modulus					1052:1058	the Young's modulus	1040:1058	the Young's modulus of the PVA/RCNCs composite film	1040:1090	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	2	33	theme	rod-shaped	422:431	arg1	RCNCs					461:465	RCNCs	461:465	RCNCs	461:465	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	2	33	theme	rod-shaped	422:431	arg1	nanocrystallines					443:458	rod-shaped cellulose nanocrystallines	422:458	rod-shaped cellulose nanocrystallines (RCNCs)	422:466	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	3	34	theme	additive	745:752	arg1	CNCs					740:743	CNCs	740:743	CNCs additive amount	740:759	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	4	35	theme	pyrolysis	883:891	arg1	°C.					911:913	pyrolysis temperature 60-80 °C.	883:913	pyrolysis temperature 60-80 °C. On the contrary	883:929	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	3	36	theme	different	603:611	arg1	morphologies					613:624	different morphologies	603:624	different morphologies	603:624	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	4	37	theme	RCNCs	992:996	arg1	films					983:987	the composite films	969:987	the composite films of RCNCs	969:996	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	1	38	theme	different	198:206	arg1	morphologies					208:219	different morphologies	198:219	different morphologies	198:219	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	2	39	theme	spherical	472:480	arg1	SCNCs					510:514	SCNCs	510:514	SCNCs	510:514	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	2	39	theme	spherical	472:480	arg1	nanocrystallines					492:507	spherical cellulose nanocrystallines	472:507	spherical cellulose nanocrystallines (SCNCs)	472:515	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	1	40	theme	PVA	309:311	arg1	matrix					313:318	PVA matrix	309:318	PVA matrix	309:318	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	1	41	with	nanocrystallines	169:184	arg1	morphologies					208:219	different morphologies	198:219	different morphologies	198:219	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	4	42	theme	films	864:868	arg1	stability					837:845	the thermal stability	825:845	the thermal stability of the composite films	825:868	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	4	43	with	comparison	1122:1131	arg1	PVA					1147:1149	the pure PVA	1138:1149	the pure PVA	1138:1149	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	3	44	theme	composite	671:679	arg1	films					681:685	the composite films	667:685	the composite films	667:685	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	0	45	dep	nanocrystals	32:43	arg1	study					77:81	A comparative study	63:81	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.	0:125	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	3	46	theme	CNCs	740:743	arg1	change					730:735	the change	726:735	the change of CNCs additive amount	726:759	The results showed CNCs with different morphologies had little effect on the transparency of the composite films, and the crystallinity fluctuated with the change of CNCs additive amount.					
33483022	2	47	theme	nanocomposite	524:536	arg1	films					538:542	PVA nanocomposite films	520:542	PVA nanocomposite films	520:542	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	2	48	theme	PVA	520:522	arg1	films					538:542	PVA nanocomposite films	520:542	PVA nanocomposite films	520:542	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	4	49	theme	film	1087:1090	arg1	modulus					1052:1058	the Young's modulus	1040:1058	the Young's modulus of the PVA/RCNCs composite film	1040:1090	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	0	50	theme	comparative	65:75	arg1	study					77:81	A comparative study	63:81	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.	0:125	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
33483022	4	51	theme	composite	1077:1085	arg1	film					1087:1090	the PVA/RCNCs composite film	1063:1090	the PVA/RCNCs composite film	1063:1090	Compared with the RCNCs, SCNCs had a better improve ability to the thermal stability of the composite films by promoting pyrolysis temperature 60-80 °C. On the contrary, the maximum mechanical properties of the composite films of RCNCs were much higher than those of SCNCs, and the Young's modulus of the PVA/RCNCs composite film were increased by 120.97 % in comparison with the pure PVA.					
33483022	1	52	theme	enzymatic	224:232	arg1	hydrolysis					234:243	enzymatic hydrolysis	224:243	enzymatic hydrolysis	224:243	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	1	53	theme	solution	324:331	arg1	casting					333:339	solution casting	324:339	solution casting	324:339	In this paper, we have isolated cellulose nanocrystallines (CNCs) with different morphologies by enzymatic hydrolysis, and prepared flexible and transparent nanocomposite films with PVA matrix via solution casting.					
33483022	2	54	theme	mechanical	387:396	arg1	methods					398:404	mechanical methods	387:404	mechanical methods	387:404	By means of SEM, UV-vis, XRD, DTG, FT-IR and mechanical methods, the effects of rod-shaped cellulose nanocrystallines (RCNCs) and spherical cellulose nanocrystallines (SCNCs) on PVA nanocomposite films were compared systematically.					
33483022	0	55	theme	reinforcing	86:96	arg1	agents					98:103	reinforcing agents	86:103	reinforcing agents	86:103	Spherical vs rod-like cellulose nanocrystals from enzymolysis: A comparative study as reinforcing agents on polyvinyl alcohol.					
34536780	0	0	theme	recommendations	67:81	arg1	digestion					9:17	In vitro digestion	0:17	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.	0:108	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	5	1	theme	digestible	827:836	arg1	content					846:852	digestible protein content	827:852	digestible protein content	827:852	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	0	2	theme	dietary	59:65	arg1	recommendations					67:81	their dietary recommendations	53:81	their dietary recommendations for different populations	53:107	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	3	3	theme	foxtail	481:487	arg1	millet					489:494	foxtail millet	481:494	foxtail millet	481:494	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	0	4	theme	different	87:95	arg1	populations					97:107	different populations	87:107	different populations	87:107	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	5	5	dep	healthier	745:753	arg1	due					767:769	due	767:769	due	767:769	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	3	6	theme	proso	502:506	arg1	millet					508:513	proso millet	502:513	proso millet	502:513	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	5	7	theme	higher	891:896	arg1	content					912:918	higher dietary fiber content	891:918	higher dietary fiber content (>12%)	891:925	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	7	theme	higher	891:896	arg1	%					924:924	>12%	921:924	>12%	921:924	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	8	theme	fiber	906:910	arg1	content					912:918	higher dietary fiber content	891:918	higher dietary fiber content (>12%)	891:925	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	8	theme	fiber	906:910	arg1	%					924:924	>12%	921:924	>12%	921:924	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	3	9	theme	in	334:335	arg1	starch					343:348	the in vitro starch and protein digestibility	330:374	starch	343:348	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	5	10	theme	content	846:852	arg1	bioaccessibility					869:884	higher digestible protein content and amino acid bioaccessibility	820:884	higher digestible protein content and amino acid bioaccessibility	820:884	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	6	11	theme	types	1024:1028	arg1	digestibility					997:1009	digestibility	997:1009	digestibility	997:1009	This study could provide a comprehensive nutritional composition and digestibility of the eight types of wholegrains and their porridges.					
34536780	6	11	theme	types	1024:1028	arg1	composition					981:991	a comprehensive nutritional composition	953:991	a comprehensive nutritional composition	953:991	This study could provide a comprehensive nutritional composition and digestibility of the eight types of wholegrains and their porridges.					
34536780	5	12	theme	glycemic	805:812	arg1	load					814:817	glycemic load	805:817	glycemic load	805:817	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	6	13	theme	nutritional	969:979	arg1	composition					981:991	a comprehensive nutritional composition	953:991	a comprehensive nutritional composition	953:991	This study could provide a comprehensive nutritional composition and digestibility of the eight types of wholegrains and their porridges.					
34536780	3	14	theme	dietary	388:394	arg1	content					402:408	dietary fiber content	388:408	dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges	388:554	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	2	15	theme	beneficial	274:283	arg1	impact					285:290	their beneficial impact	268:290	their beneficial impact on health	268:300	However, different wholegrains vary in nutritional composition and their beneficial impact on health.					
34536780	3	16	theme	different	419:427	arg1	wholegrains					429:439	eight different wholegrains	413:439	eight different wholegrains	413:439	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	7	17	theme	different	1110:1118	arg1	populations					1120:1130	different populations	1110:1130	different populations based on factor analysis	1110:1155	Dietary recommendations were also given for different populations based on factor analysis.					
34536780	1	18	theme	various	176:182	arg1	benefits					191:198	their various health benefits	170:198	their various health benefits	170:198	Wholegrains have been promoted for human consumption due to their various health benefits.					
34536780	0	19	theme	In	0:1	arg1	digestion					9:17	In vitro digestion	0:17	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.	0:108	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	5	20	theme	higher	820:825	arg1	bioaccessibility					869:884	higher digestible protein content and amino acid bioaccessibility	820:884	higher digestible protein content and amino acid bioaccessibility	820:884	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	2	21	from	composition	252:262	arg1	health					295:300	health	295:300	health	295:300	However, different wholegrains vary in nutritional composition and their beneficial impact on health.					
34536780	1	22	theme	health	184:189	arg1	benefits					191:198	their various health benefits	170:198	their various health benefits	170:198	Wholegrains have been promoted for human consumption due to their various health benefits.					
34536780	3	23	theme	protein	354:360	arg1	digestibility					362:374	the in vitro starch and protein digestibility	330:374	digestibility	362:374	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	6	24	theme	comprehensive	955:967	arg1	composition					981:991	a comprehensive nutritional composition	953:991	a comprehensive nutritional composition	953:991	This study could provide a comprehensive nutritional composition and digestibility of the eight types of wholegrains and their porridges.					
34536780	5	25	theme	protein	838:844	arg1	content					846:852	digestible protein content	827:852	digestible protein content	827:852	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	6	26	theme	wholegrains	1033:1043	arg1	types					1024:1028	the eight types	1014:1028	the eight types of wholegrains and their porridges	1014:1063	This study could provide a comprehensive nutritional composition and digestibility of the eight types of wholegrains and their porridges.					
34536780	0	27	theme	types	28:32	arg1	digestion					9:17	In vitro digestion	0:17	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.	0:108	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	6	28	theme	porridges	1055:1063	arg1	types					1024:1028	the eight types	1014:1028	the eight types of wholegrains and their porridges	1014:1063	This study could provide a comprehensive nutritional composition and digestibility of the eight types of wholegrains and their porridges.					
34536780	5	29	theme	lower	780:784	arg1	index					795:799	lower glycemic index	780:799	lower glycemic index	780:799	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	4	30	theme	grains	616:621	arg1	digestibility					636:648	protein digestibility	628:648	protein digestibility	628:648	We found that boiling improved starch digestibility of all grains, and protein digestibility except proso millet and sorghum.					
34536780	4	30	theme	grains	616:621	arg1	digestibility					595:607	starch digestibility	588:607	starch digestibility of all grains	588:621	We found that boiling improved starch digestibility of all grains, and protein digestibility except proso millet and sorghum.					
34536780	3	31	theme	wholegrains	429:439	arg1	content					402:408	dietary fiber content	388:408	dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges	388:554	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	3	31	theme	wholegrains	429:439	arg1	starch					343:348	the in vitro starch and protein digestibility	330:374	starch	343:348	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	3	31	theme	wholegrains	429:439	arg1	digestibility					362:374	the in vitro starch and protein digestibility	330:374	digestibility	362:374	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	3	32	theme	fiber	396:400	arg1	content					402:408	dietary fiber content	388:408	dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges	388:554	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	4	33	theme	starch	588:593	arg1	digestibility					595:607	starch digestibility	588:607	starch digestibility of all grains	588:621	We found that boiling improved starch digestibility of all grains, and protein digestibility except proso millet and sorghum.					
34536780	0	34	theme	wholegrains	37:47	arg1	types					28:32	eight types	22:32	eight types of wholegrains	22:47	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	0	34	theme	wholegrains	37:47	arg1	recommendations					67:81	their dietary recommendations	53:81	their dietary recommendations for different populations	53:107	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	7	35	theme	factor	1141:1146	arg1	analysis					1148:1155	factor analysis	1141:1155	factor analysis	1141:1155	Dietary recommendations were also given for different populations based on factor analysis.					
34536780	3	36	dep	in	334:335	arg1	vitro					337:341	vitro	337:341	vitro	337:341	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	3	37	theme	coix	470:473	arg1	seed					475:478	coix seed	470:478	coix seed	470:478	In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges.					
34536780	2	38	theme	different	210:218	arg1	wholegrains					220:230	different wholegrains	210:230	different wholegrains	210:230	However, different wholegrains vary in nutritional composition and their beneficial impact on health.					
34536780	4	39	theme	proso	657:661	arg1	millet					663:668	proso millet	657:668	proso millet	657:668	We found that boiling improved starch digestibility of all grains, and protein digestibility except proso millet and sorghum.					
34536780	2	40	from	impact	285:290	arg1	health					295:300	health	295:300	health	295:300	However, different wholegrains vary in nutritional composition and their beneficial impact on health.					
34536780	0	41	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.					
34536780	5	42	theme	amino	858:862	arg1	acid					864:867	amino acid	858:867	amino acid	858:867	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	43	theme	dietary	898:904	arg1	content					912:918	higher dietary fiber content	891:918	higher dietary fiber content (>12%)	891:925	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	43	theme	dietary	898:904	arg1	%					924:924	>12%	921:924	>12%	921:924	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	44	theme	acid	864:867	arg1	bioaccessibility					869:884	higher digestible protein content and amino acid bioaccessibility	820:884	higher digestible protein content and amino acid bioaccessibility	820:884	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	5	45	theme	glycemic	786:793	arg1	index					795:799	lower glycemic index	780:799	lower glycemic index	780:799	Porridges made from oats, quinoa, or buckwheat are considered healthier than others due to their lower glycemic index and glycemic load, higher digestible protein content and amino acid bioaccessibility, and higher dietary fiber content (>12%).					
34536780	4	46	theme	protein	628:634	arg1	digestibility					636:648	protein digestibility	628:648	protein digestibility	628:648	We found that boiling improved starch digestibility of all grains, and protein digestibility except proso millet and sorghum.					
34536780	7	47	theme	Dietary	1066:1072	arg1	recommendations					1074:1088	Dietary recommendations	1066:1088	Dietary recommendations	1066:1088	Dietary recommendations were also given for different populations based on factor analysis.					
34536780	2	48	theme	nutritional	240:250	arg1	composition					252:262	nutritional composition	240:262	nutritional composition	240:262	However, different wholegrains vary in nutritional composition and their beneficial impact on health.					
34536780	1	49	theme	human	145:149	arg1	consumption					151:161	human consumption	145:161	human consumption due to their various health benefits	145:198	Wholegrains have been promoted for human consumption due to their various health benefits.					
34810018	3	0	from	levels	433:438	arg1	samples					506:512	coated samples	499:512	coated samples	499:512	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	5	1	dep	showed	865:870	arg1	contributed					891:901	contributed	891:901	showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella	865:972	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	1	2	from	effects	157:163	arg1	retention					272:280	quality retention	264:280	quality retention of fish flesh during refrigeration	264:315	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	5	3	theme	throughput	826:835	arg1	sequencing					837:846	high throughput sequencing	821:846	high throughput sequencing analysis	821:855	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	0	4	theme	flesh	106:110	arg1	preservation					112:123	fish flesh preservation	101:123	fish flesh preservation	101:123	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate peel extract for fish flesh preservation.					
34810018	3	5	dep	decreased	527:535	arg1	p < 0.05					538:545	p < 0.05	538:545	p < 0.05	538:545	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	4	6	from	softening	687:695	arg1	fillets					700:706	fillets	700:706	fillets	700:706	Meanwhile, the coating also helped inhibit oxidation, bioamine accumulation, and texture softening in fillets.					
34810018	4	7	theme	texture	679:685	arg1	softening					687:695	texture softening	679:695	texture softening	679:695	Meanwhile, the coating also helped inhibit oxidation, bioamine accumulation, and texture softening in fillets.					
34810018	0	8	theme	fish	101:104	arg1	preservation					112:123	fish flesh preservation	101:123	fish flesh preservation	101:123	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate peel extract for fish flesh preservation.					
34810018	5	9	from	Control	789:795	arg1	day					800:802	day 6	800:804	day 6 afterward	800:814	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	1	10	theme	quality	264:270	arg1	retention					272:280	quality retention	264:280	quality retention of fish flesh during refrigeration	264:315	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	6	11	theme	positive	993:1000	arg1	effect					1002:1007	the positive effect	989:1007	the positive effect of the coating scheme	989:1029	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	5	12	theme	active	876:881	arg1	coating					883:889	the active coating	872:889	the active coating	872:889	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	1	13	theme	vacuum-impregnated	168:185	arg1	chitosan					201:208	vacuum-impregnated carboxymethyl chitosan	168:208	vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE)	168:259	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	1	13	theme	vacuum-impregnated	168:185	arg1	CMCS					211:214	CMCS	211:214	CMCS	211:214	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	3	14	theme	coated	499:504	arg1	samples					506:512	coated samples	499:512	coated samples	499:512	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	3	15	from	%	562:562	arg1	day					577:579	day 9	577:581	day 9	577:581	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	1	16	theme	carboxymethyl	187:199	arg1	chitosan					201:208	vacuum-impregnated carboxymethyl chitosan	168:208	vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE)	168:259	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	1	16	theme	carboxymethyl	187:199	arg1	CMCS					211:214	CMCS	211:214	CMCS	211:214	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	0	17	theme	bioactive	16:24	arg1	coatings					26:33	Multifunctional bioactive coatings	0:33	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate	0:82	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate peel extract for fish flesh preservation.					
34810018	4	18	from	accumulation	661:672	arg1	fillets					700:706	fillets	700:706	fillets	700:706	Meanwhile, the coating also helped inhibit oxidation, bioamine accumulation, and texture softening in fillets.					
34810018	4	19	theme	bioamine	652:659	arg1	accumulation					661:672	bioamine accumulation	652:672	bioamine accumulation	652:672	Meanwhile, the coating also helped inhibit oxidation, bioamine accumulation, and texture softening in fillets.					
34810018	1	20	theme	chitosan	201:208	arg1	effects					157:163	the effects	153:163	the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration	153:315	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	0	21	theme	Multifunctional	0:14	arg1	coatings					26:33	Multifunctional bioactive coatings	0:33	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate	0:82	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate peel extract for fish flesh preservation.					
34810018	5	22	theme	sequencing	837:846	arg1	analysis					848:855	high throughput sequencing analysis	821:855	high throughput sequencing analysis	821:855	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	5	23	theme	microbial	723:731	arg1	enumeration					733:743	the microbial enumeration	719:743	the microbial enumeration	719:743	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	5	24	theme	high	821:824	arg1	sequencing					837:846	high throughput sequencing	821:846	high throughput sequencing analysis	821:855	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	6	25	theme	longsnout	1052:1060	arg1	catfish					1062:1068	longsnout catfish	1052:1068	longsnout catfish	1052:1068	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	3	26	theme	loss	448:451	arg1	levels					433:438	the levels	429:438	the levels of drip loss, total volatile base nitrogen, and K value in coated samples	429:512	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	6	27	theme	scheme	1024:1029	arg1	effect					1002:1007	the positive effect	989:1007	the positive effect of the coating scheme	989:1029	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	1	28	theme	fish	285:288	arg1	flesh					290:294	fish flesh	285:294	fish flesh	285:294	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	3	29	theme	K	488:488	arg1	value					490:494	K value	488:494	K value	488:494	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	0	30	theme	water-soluble	44:56	arg1	chitosan					58:65	water-soluble chitosan	44:65	water-soluble chitosan with pomegranate	44:82	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate peel extract for fish flesh preservation.					
34810018	1	31	theme	flesh	290:294	arg1	retention					272:280	quality retention	264:280	quality retention of fish flesh during refrigeration	264:315	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	5	32	theme	growth	918:923	arg1	suppression					925:935	the notable growth suppression	906:935	the notable growth suppression of spoilage bacteria like Shewanella	906:972	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	3	33	from	%	555:555	arg1	day					577:579	day 9	577:581	day 9	577:581	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	2	34	theme	carp	394:397	arg1	fillets					399:405	grass carp fillets	388:405	grass carp fillets	388:405	Herein, CMCS-PPE coating was effective in attenuating quality loss of grass carp fillets.					
34810018	5	35	theme	lg	765:766	arg1	cfu/g					768:772	>1.4 lg cfu/g	760:772	>1.4 lg cfu/g	760:772	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	2	36	theme	fillets	399:405	arg1	loss					380:383	quality loss	372:383	quality loss of grass carp fillets	372:405	Herein, CMCS-PPE coating was effective in attenuating quality loss of grass carp fillets.					
34810018	6	37	theme	flesh	1136:1140	arg1	preservation					1142:1153	fish flesh preservation	1131:1153	fish flesh preservation	1131:1153	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	6	38	theme	coating	1016:1022	arg1	scheme					1024:1029	the coating scheme	1012:1029	the coating scheme	1012:1029	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	3	39	theme	base	469:472	arg1	nitrogen					474:481	total volatile base nitrogen	454:481	total volatile base nitrogen	454:481	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	3	40	theme	total	454:458	arg1	nitrogen					474:481	total volatile base nitrogen	454:481	total volatile base nitrogen	454:481	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	5	41	theme	bacteria	949:956	arg1	suppression					925:935	the notable growth suppression	906:935	the notable growth suppression of spoilage bacteria like Shewanella	906:972	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	5	42	theme	spoilage	940:947	arg1	bacteria					949:956	spoilage bacteria	940:956	spoilage bacteria like Shewanella	940:972	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	3	43	theme	nitrogen	474:481	arg1	levels					433:438	the levels	429:438	the levels of drip loss, total volatile base nitrogen, and K value in coated samples	429:512	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	4	44	from	oxidation	641:649	arg1	fillets					700:706	fillets	700:706	fillets	700:706	Meanwhile, the coating also helped inhibit oxidation, bioamine accumulation, and texture softening in fillets.					
34810018	2	45	theme	CMCS-PPE	326:333	arg1	coating					335:341	CMCS-PPE coating	326:341	CMCS-PPE coating	326:341	Herein, CMCS-PPE coating was effective in attenuating quality loss of grass carp fillets.					
34810018	1	46	theme	pomegranate	230:240	arg1	extract					247:253	pomegranate peel extract	230:253	pomegranate peel extract (PPE)	230:259	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	1	46	theme	pomegranate	230:240	arg1	PPE					256:258	PPE	256:258	PPE	256:258	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	3	47	theme	volatile	460:467	arg1	nitrogen					474:481	total volatile base nitrogen	454:481	total volatile base nitrogen	454:481	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	1	48	theme	peel	242:245	arg1	extract					247:253	pomegranate peel extract	230:253	pomegranate peel extract (PPE)	230:259	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	1	48	theme	peel	242:245	arg1	PPE					256:258	PPE	256:258	PPE	256:258	This study aimed to reveal the effects of vacuum-impregnated carboxymethyl chitosan (CMCS) coating with pomegranate peel extract (PPE) on quality retention of fish flesh during refrigeration.					
34810018	3	49	theme	drip	443:446	arg1	loss					448:451	drip loss	443:451	drip loss	443:451	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	6	50	theme	good	1108:1111	arg1	applicability					1113:1125	its good applicability	1104:1125	its good applicability for fish flesh preservation	1104:1153	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	0	51	with	chitosan	58:65	arg1	pomegranate					72:82	pomegranate	72:82	pomegranate	72:82	Multifunctional bioactive coatings based on water-soluble chitosan with pomegranate peel extract for fish flesh preservation.					
34810018	2	52	theme	quality	372:378	arg1	loss					380:383	quality loss	372:383	quality loss of grass carp fillets	372:405	Herein, CMCS-PPE coating was effective in attenuating quality loss of grass carp fillets.					
34810018	2	53	theme	grass	388:392	arg1	carp					394:397	grass carp	388:397	grass carp fillets	388:405	Herein, CMCS-PPE coating was effective in attenuating quality loss of grass carp fillets.					
34810018	5	54	theme	notable	910:916	arg1	suppression					925:935	the notable growth suppression	906:935	the notable growth suppression of spoilage bacteria like Shewanella	906:972	Moreover, the microbial enumeration was reduced by >1.4 lg cfu/g as compared to Control on day 6 afterward, and high throughput sequencing analysis further showed the active coating contributed to the notable growth suppression of spoilage bacteria like Shewanella.					
34810018	6	55	theme	fish	1131:1134	arg1	preservation					1142:1153	fish flesh preservation	1131:1153	fish flesh preservation	1131:1153	Additionally, the positive effect of the coating scheme was also verified in longsnout catfish and snakehead, further confirming its good applicability for fish flesh preservation.					
34810018	3	56	theme	value	490:494	arg1	levels					433:438	the levels	429:438	the levels of drip loss, total volatile base nitrogen, and K value in coated samples	429:512	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
34810018	3	57	from	%	572:572	arg1	day					577:579	day 9	577:581	day 9	577:581	Compared to Control, the levels of drip loss, total volatile base nitrogen, and K value in coated samples were sharply decreased (p < 0.05) by 24.5%, 35.3% and 25.2% on day 9, respectively.					
32861784	0	0	theme	reactive	77:84	arg1	dye					94:96	reactive blue 19 dye	77:96	reactive blue 19 dye	77:96	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	2	1	theme	composite	504:512	arg1	product					436:442	The final product	426:442	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	426:512	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	6	2	theme	adsorption	1258:1267	arg1	process					1269:1275	the RB19 adsorption process	1249:1275	the RB19 adsorption process	1249:1275	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	3	3	theme	Box-Behnken	906:916	arg1	RSM-BBD					926:932	RSM-BBD	926:932	RSM-BBD	926:932	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	3	theme	Box-Behnken	906:916	arg1	design					918:923	Box-Behnken design	906:923	Box-Behnken design (RSM-BBD)	906:933	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	1	4	theme	current	163:169	arg1	work					180:183	current research work	163:183	current research work	163:183	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	3	5	theme	color	802:806	arg1	removal					808:814	the RB19 color removal	793:814	the RB19 color removal	793:814	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	1	6	theme	chemical	242:249	arg1	modifications					251:263	subsequent physical and chemical modifications	218:263	subsequent physical and chemical modifications	218:263	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	2	7	theme	oxygen	570:575	arg1	COD					585:587	COD	585:587	COD	585:587	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	7	theme	oxygen	570:575	arg1	demand					577:582	oxygen demand	570:582	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	7	8	theme	n-π	1454:1456	arg1	interactions					1458:1469	n-π interactions	1454:1469	n-π interactions	1454:1469	The mechanism of RB19 adsorption onto the Chi-ECH/KA may include electrostatic interactions, hydrogen bonding, Yoshida H-bonding, and n-π interactions.					
32861784	2	9	theme	dye	611:613	arg1	reduction					590:598	chemical oxygen demand (COD) reduction	561:598	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	9	theme	dye	611:613	arg1	removal					549:555	color removal	543:555	color removal	543:555	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	8	10	theme	composite	1564:1572	arg1	composite					1505:1513	Chi-ECH/KA composite	1494:1513	Chi-ECH/KA composite	1494:1513	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	10	theme	composite	1564:1572	arg1	bio					1574:1576	an eco-friendly, potential and multi-function composite bio	1518:1576	an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment	1518:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	1	11	theme	research	171:178	arg1	work					180:183	current research work	163:183	current research work	163:183	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	8	12	theme	COD	1619:1621	arg1	reduction					1623:1631	COD reduction	1619:1631	COD reduction	1619:1631	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	13	theme	Chi-ECH/KA	1494:1503	arg1	composite					1505:1513	Chi-ECH/KA composite	1494:1513	Chi-ECH/KA composite	1494:1513	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	13	theme	Chi-ECH/KA	1494:1503	arg1	bio					1574:1576	an eco-friendly, potential and multi-function composite bio	1518:1576	an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment	1518:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	2	14	theme	textile	603:609	arg1	dye					611:613	textile dye	603:613	textile dye	603:613	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	6	15	theme	thermodynamic	1210:1222	arg1	functions					1224:1232	The calculated thermodynamic functions	1195:1232	The calculated thermodynamic functions clarified that the RB19 adsorption process	1195:1275	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	6	15	theme	thermodynamic	1210:1222	arg1	endothermic					1281:1291	endothermic	1281:1291	endothermic	1281:1291	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	8	16	theme	aqueous	1638:1644	arg1	environment					1646:1656	aqueous environment	1638:1656	aqueous environment	1638:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	0	17	theme	blue	86:89	arg1	dye					94:96	reactive blue 19 dye	77:96	reactive blue 19 dye	77:96	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	3	18	theme	COD	820:822	arg1	reduction					824:832	COD reduction	820:832	COD reduction	820:832	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	19	with	methodology	889:899	arg1	RSM-BBD					926:932	RSM-BBD	926:932	RSM-BBD	926:932	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	19	with	methodology	889:899	arg1	design					918:923	Box-Behnken design	906:923	Box-Behnken design (RSM-BBD)	906:933	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	4	20	theme	adsorption	965:974	arg1	data					953:956	The experimental data	936:956	The experimental data of the adsorption kinetic and the adsorption isotherm	936:1010	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	4	21	theme	order	1059:1063	arg1	model					1065:1069	pseudo-second order model	1045:1069	pseudo-second order model	1045:1069	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	6	22	theme	calculated	1199:1208	arg1	functions					1224:1232	The calculated thermodynamic functions	1195:1232	The calculated thermodynamic functions clarified that the RB19 adsorption process	1195:1275	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	6	22	theme	calculated	1199:1208	arg1	endothermic					1281:1291	endothermic	1281:1291	endothermic	1281:1291	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	0	23	theme	dye	94:96	arg1	removal					48:54	color removal	42:54	color removal	42:54	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	0	23	theme	dye	94:96	arg1	reduction					64:72	COD reduction	60:72	COD reduction	60:72	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	3	24	from	influence	680:688	arg1	removal					808:814	the RB19 color removal	793:814	the RB19 color removal	793:814	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	24	from	influence	680:688	arg1	reduction					824:832	COD reduction	820:832	COD reduction	820:832	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	2	25	dep	reduction	590:598	arg1	RB19					644:647	RB19	644:647	RB19	644:647	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	25	dep	reduction	590:598	arg1	dye					639:641	namely reactive blue 19 dye	615:641	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	4	26	theme	experimental	940:951	arg1	data					953:956	The experimental data	936:956	The experimental data of the adsorption kinetic and the adsorption isotherm	936:1010	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	4	27	theme	Langmuir	1075:1082	arg1	model					1093:1097	Langmuir isotherm model	1075:1097	Langmuir isotherm model	1075:1097	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	1	28	theme	covalent	361:368	arg1	cross-linker					370:381	a covalent cross-linker	359:381	a covalent cross-linker namely epichlorohydrin (ECH) respectively	359:423	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	2	29	theme	blue	631:634	arg1	RB19					644:647	RB19	644:647	RB19	644:647	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	29	theme	blue	631:634	arg1	dye					639:641	namely reactive blue 19 dye	615:641	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	30	link	crosslinked	447:457	arg1	composite					504:512	crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	447:512	crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	447:512	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	7	31	theme	electrostatic	1385:1397	arg1	interactions					1399:1410	electrostatic interactions	1385:1410	electrostatic interactions	1385:1410	The mechanism of RB19 adsorption onto the Chi-ECH/KA may include electrostatic interactions, hydrogen bonding, Yoshida H-bonding, and n-π interactions.					
32861784	0	32	theme	Statistical	0:10	arg1	optimization					12:23	Statistical optimization	0:23	Statistical optimization	0:23	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	1	33	theme	kaolin	282:287	arg1	KA					295:296	KA	295:296	KA	295:296	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	1	33	theme	kaolin	282:287	arg1	clay					289:292	kaolin clay	282:292	kaolin clay (KA)	282:297	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	2	34	theme	reactive	622:629	arg1	RB19					644:647	RB19	644:647	RB19	644:647	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	34	theme	reactive	622:629	arg1	dye					639:641	namely reactive blue 19 dye	615:641	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	6	35	theme	RB19	1253:1256	arg1	process					1269:1275	the RB19 adsorption process	1249:1275	the RB19 adsorption process	1249:1275	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	2	36	theme	final	430:434	arg1	product					436:442	The final product	426:442	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	426:512	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	5	37	theme	absorption	1123:1132	arg1	ability					1134:1140	Excellent absorption ability	1113:1140	Excellent absorption ability of 560.9 mg/g	1113:1154	Excellent absorption ability of 560.9 mg/g was recorded for Chi-ECH/KA composite.					
32861784	0	38	theme	chitosan-epichlorohydrin/kaolin	112:142	arg1	composite					149:157	mesoporous chitosan-epichlorohydrin/kaolin clay composite	101:157	mesoporous chitosan-epichlorohydrin/kaolin clay composite	101:157	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	2	39	theme	aqueous	655:661	arg1	environment					663:673	aqueous environment	655:673	aqueous environment	655:673	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	4	40	theme	better	1027:1032	arg1	fitness					1034:1040	a better fitness	1025:1040	a better fitness to pseudo-second order model and Langmuir isotherm model	1025:1097	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	3	41	theme	Chi-ECH/KA	723:732	arg1	0.02-0.1 g					740:749	0.02-0.1 g	740:749	0.02-0.1 g	740:749	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	41	theme	Chi-ECH/KA	723:732	arg1	dose					734:737	Chi-ECH/KA dose	723:737	Chi-ECH/KA dose (0.02-0.1 g)	723:750	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	0	42	theme	mesoporous	101:110	arg1	composite					149:157	mesoporous chitosan-epichlorohydrin/kaolin clay composite	101:157	mesoporous chitosan-epichlorohydrin/kaolin clay composite	101:157	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	4	43	theme	pseudo-second	1045:1057	arg1	model					1065:1069	pseudo-second order model	1045:1069	pseudo-second order model	1045:1069	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	0	44	theme	color	42:46	arg1	removal					48:54	color removal	42:54	color removal	42:54	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	6	45	from	nature	1312:1317	arg1	endothermic					1281:1291	endothermic	1281:1291	endothermic	1281:1291	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	6	45	from	nature	1312:1317	arg1	functions					1224:1232	The calculated thermodynamic functions	1195:1232	The calculated thermodynamic functions clarified that the RB19 adsorption process	1195:1275	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	2	46	theme	chemical	561:568	arg1	reduction					590:598	chemical oxygen demand (COD) reduction	561:598	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	8	47	theme	reduction	1623:1631	arg1	removal					1592:1598	removal	1592:1598	removal of textile dye and COD reduction from aqueous environment	1592:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	48	from	environment	1646:1656	arg1	removal					1592:1598	removal	1592:1598	removal of textile dye and COD reduction from aqueous environment	1592:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	49	theme	adsorbent	1578:1586	arg1	composite					1505:1513	Chi-ECH/KA composite	1494:1513	Chi-ECH/KA composite	1494:1513	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	49	theme	adsorbent	1578:1586	arg1	bio					1574:1576	an eco-friendly, potential and multi-function composite bio	1518:1576	an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment	1518:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	0	50	theme	clay	144:147	arg1	composite					149:157	mesoporous chitosan-epichlorohydrin/kaolin clay composite	101:157	mesoporous chitosan-epichlorohydrin/kaolin clay composite	101:157	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	1	51	theme	physical	229:236	arg1	modifications					251:263	subsequent physical and chemical modifications	218:263	subsequent physical and chemical modifications	218:263	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	3	52	theme	surface	881:887	arg1	methodology					889:899	response surface methodology	872:899	response surface methodology with Box-Behnken design (RSM-BBD)	872:933	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	6	53	from	endothermic	1281:1291	arg1	nature					1312:1317	nature	1312:1317	nature	1312:1317	The calculated thermodynamic functions clarified that the RB19 adsorption process was endothermic and spontaneous in nature.					
32861784	2	54	theme	chitosan-epichlorohydrin/kaolin	459:489	arg1	composite					504:512	crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	447:512	crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	447:512	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	8	55	theme	eco-friendly	1521:1532	arg1	composite					1505:1513	Chi-ECH/KA composite	1494:1513	Chi-ECH/KA composite	1494:1513	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	55	theme	eco-friendly	1521:1532	arg1	bio					1574:1576	an eco-friendly, potential and multi-function composite bio	1518:1576	an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment	1518:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	56	theme	potential	1535:1543	arg1	composite					1505:1513	Chi-ECH/KA composite	1494:1513	Chi-ECH/KA composite	1494:1513	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	56	theme	potential	1535:1543	arg1	bio					1574:1576	an eco-friendly, potential and multi-function composite bio	1518:1576	an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment	1518:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	7	57	theme	adsorption	1342:1351	arg1	mechanism					1324:1332	The mechanism	1320:1332	The mechanism of RB19 adsorption onto the Chi-ECH/KA	1320:1371	The mechanism of RB19 adsorption onto the Chi-ECH/KA may include electrostatic interactions, hydrogen bonding, Yoshida H-bonding, and n-π interactions.					
32861784	2	58	theme	crosslinked	447:457	arg1	composite					504:512	crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	447:512	crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite	447:512	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	3	59	dep	A	720:720	arg1	5-30 min					780:787	5-30 min	780:787	5-30 min	780:787	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	59	dep	A	720:720	arg1	i.e.					715:718	i.e.	715:718	i.e.	715:718	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	3	59	dep	A	720:720	arg1	time					774:777	time	774:777	A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min)	720:788	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	2	60	theme	demand	577:582	arg1	reduction					590:598	chemical oxygen demand (COD) reduction	561:598	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	8	61	theme	multi-function	1549:1562	arg1	composite					1505:1513	Chi-ECH/KA composite	1494:1513	Chi-ECH/KA composite	1494:1513	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	8	61	theme	multi-function	1549:1562	arg1	bio					1574:1576	an eco-friendly, potential and multi-function composite bio	1518:1576	an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment	1518:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	4	62	theme	isotherm	1003:1010	arg1	adsorption					965:974	the adsorption	961:974	the adsorption kinetic and the adsorption isotherm	961:1010	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	1	63	theme	polymeric	308:316	arg1	structure					318:326	its polymeric structure	304:326	its polymeric structure	304:326	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	3	64	theme	response	872:879	arg1	methodology					889:899	response surface methodology	872:899	response surface methodology with Box-Behnken design (RSM-BBD)	872:933	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	4	65	theme	adsorption	992:1001	arg1	isotherm					1003:1010	the adsorption isotherm	988:1010	the adsorption isotherm	988:1010	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	5	66	theme	Chi-ECH/KA	1173:1182	arg1	composite					1184:1192	Chi-ECH/KA composite	1173:1192	Chi-ECH/KA composite	1173:1192	Excellent absorption ability of 560.9 mg/g was recorded for Chi-ECH/KA composite.					
32861784	7	67	theme	hydrogen	1413:1420	arg1	bonding					1422:1428	hydrogen bonding	1413:1428	hydrogen bonding	1413:1428	The mechanism of RB19 adsorption onto the Chi-ECH/KA may include electrostatic interactions, hydrogen bonding, Yoshida H-bonding, and n-π interactions.					
32861784	7	68	theme	RB19	1337:1340	arg1	adsorption					1342:1351	RB19 adsorption	1337:1351	RB19 adsorption onto the Chi-ECH/KA	1337:1371	The mechanism of RB19 adsorption onto the Chi-ECH/KA may include electrostatic interactions, hydrogen bonding, Yoshida H-bonding, and n-π interactions.					
32861784	5	69	theme	560.9 mg/g	1145:1154	arg1	ability					1134:1140	Excellent absorption ability	1113:1140	Excellent absorption ability of 560.9 mg/g	1113:1154	Excellent absorption ability of 560.9 mg/g was recorded for Chi-ECH/KA composite.					
32861784	1	70	theme	subsequent	218:227	arg1	modifications					251:263	subsequent physical and chemical modifications	218:263	subsequent physical and chemical modifications	218:263	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	4	71	theme	kinetic	976:982	arg1	adsorption					965:974	the adsorption	961:974	the adsorption kinetic and the adsorption isotherm	961:1010	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	4	72	theme	isotherm	1084:1091	arg1	model					1093:1097	Langmuir isotherm model	1075:1097	Langmuir isotherm model	1075:1097	The experimental data of the adsorption kinetic and the adsorption isotherm demonstrated a better fitness to pseudo-second order model and Langmuir isotherm model respectively.					
32861784	2	73	from	environment	663:673	arg1	reduction					590:598	chemical oxygen demand (COD) reduction	561:598	chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19)	561:648	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	2	73	from	environment	663:673	arg1	removal					549:555	color removal	543:555	color removal	543:555	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
32861784	5	74	theme	Excellent	1113:1121	arg1	ability					1134:1140	Excellent absorption ability	1113:1140	Excellent absorption ability of 560.9 mg/g	1113:1154	Excellent absorption ability of 560.9 mg/g was recorded for Chi-ECH/KA composite.					
32861784	1	75	dep	cross-linker	370:381	arg1	epichlorohydrin					390:404	epichlorohydrin	390:404	a covalent cross-linker namely epichlorohydrin (ECH) respectively	359:423	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	1	75	dep	cross-linker	370:381	arg1	ECH					407:409	ECH	407:409	ECH	407:409	In current research work, chitosan (Chi) was subjected to subsequent physical and chemical modifications by incorporating kaolin clay (KA) into its polymeric structure, and crosslinking process with a covalent cross-linker namely epichlorohydrin (ECH) respectively.					
32861784	0	76	theme	COD	60:62	arg1	reduction					64:72	COD reduction	60:72	COD reduction	60:72	Statistical optimization and modeling for color removal and COD reduction of reactive blue 19 dye by mesoporous chitosan-epichlorohydrin/kaolin clay composite.					
32861784	3	77	theme	pertinent	693:701	arg1	parameters					703:712	pertinent parameters	693:712	pertinent parameters	693:712	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	8	78	theme	dye	1611:1613	arg1	removal					1592:1598	removal	1592:1598	removal of textile dye and COD reduction from aqueous environment	1592:1656	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	3	79	theme	parameters	703:712	arg1	influence					680:688	The influence	676:688	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction	676:832	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	8	80	theme	textile	1603:1609	arg1	dye					1611:1613	textile dye	1603:1613	textile dye	1603:1613	This study introduces Chi-ECH/KA composite as an eco-friendly, potential and multi-function composite bio adsorbent for removal of textile dye and COD reduction from aqueous environment.					
32861784	3	81	theme	RB19	797:800	arg1	removal					808:814	the RB19 color removal	793:814	the RB19 color removal	793:814	The influence of pertinent parameters, i.e. A: Chi-ECH/KA dose (0.02-0.1 g), B: pH (4-10), and C: time (5-30 min) on the RB19 color removal and COD reduction were statistically optimized by using response surface methodology with Box-Behnken design (RSM-BBD).					
32861784	2	82	theme	color	543:547	arg1	removal					549:555	color removal	543:555	color removal	543:555	The final product of crosslinked chitosan-epichlorohydrin/kaolin (Chi-ECH/KA) composite was successfully applied for color removal and chemical oxygen demand (COD) reduction of textile dye namely reactive blue 19 dye (RB19) from aqueous environment.					
34044916	3	0	theme	16S	340:342	arg1	sequencing					349:358	16S rDNA sequencing	340:358	16S rDNA sequencing	340:358	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	2	1	theme	nanocellulose	132:144	arg1	production					146:155	Bacterial nanocellulose production	122:155	Bacterial nanocellulose production	122:155	Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry.					
34044916	9	2	theme	Physical	938:945	arg1	properties					947:956	Physical properties	938:956	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature	938:1031	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	9	2	theme	Physical	938:945	arg1	crystallinity					966:978	crystallinity	966:978	crystallinity	966:978	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	9	2	theme	Physical	938:945	arg1	dimensions					988:997	fibril dimensions	981:997	fibril dimensions	981:997	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	9	2	theme	Physical	938:945	arg1	temperature					1021:1031	glass transition temperature	1004:1031	glass transition temperature	1004:1031	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	2	3	from	applications	192:203	arg1	cosmetics					214:222	cosmetics	214:222	cosmetics	214:222	Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry.					
34044916	2	3	from	applications	192:203	arg1	industry					236:243	medical industry	228:243	medical industry	228:243	Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry.					
34044916	2	3	from	applications	192:203	arg1	food					208:211	food	208:211	food	208:211	Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry.					
34044916	2	4	theme	Bacterial	122:130	arg1	production					146:155	Bacterial nanocellulose production	122:155	Bacterial nanocellulose production	122:155	Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry.					
34044916	11	5	theme	cosmetics	1299:1307	arg1	field					1290:1294	the field	1286:1294	the field of cosmetics, composite and wound care	1286:1333	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	8	6	theme	Average	867:873	arg1	rate					911:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate was 45 mg/L/day each.					
34044916	8	7	theme	nitrogen	875:882	arg1	rate					911:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate was 45 mg/L/day each.					
34044916	10	8	theme	carbon	1126:1131	arg1	glucose					1105:1111	glucose	1105:1111	glucose	1105:1111	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	10	8	theme	carbon	1126:1131	arg1	glycerol					1092:1099	glycerol	1092:1099	glycerol	1092:1099	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	10	8	theme	carbon	1126:1131	arg1	source					1133:1138	carbon source	1126:1138	carbon source	1126:1138	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	11	9	theme	Renewable	1174:1182	arg1	cellulose					1212:1220	bacterial cellulose	1202:1220	bacterial cellulose	1202:1220	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	11	9	theme	Renewable	1174:1182	arg1	materials					1184:1192	Renewable materials	1174:1192	Renewable materials such as bacterial cellulose with their unique properties	1174:1249	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	11	9	theme	Renewable	1174:1182	arg1	future					1259:1264	future	1259:1264	future	1259:1264	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	5	10	theme	first	520:524	arg1	This					508:511	This	508:511	This	508:511	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	10	theme	first	520:524	arg1	report					526:531	the first report	516:531	the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied	516:674	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	9	11	theme	fibril	981:986	arg1	dimensions					988:997	fibril dimensions	981:997	fibril dimensions	981:997	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	11	12	theme	wound	1324:1328	arg1	care					1330:1333	wound care	1324:1333	wound care	1324:1333	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	6	13	theme	optimal	705:711	arg1	concentration					713:725	optimal concentration	705:725	optimal concentration	705:725	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	3	14	theme	rDNA	344:347	arg1	sequencing					349:358	16S rDNA sequencing	340:358	16S rDNA sequencing	340:358	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	3	15	theme	Acetobacter	252:262	arg1	strains					264:270	Three Acetobacter strains	246:270	Three Acetobacter strains isolated from organic waste and fermented tea	246:316	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	1	16	theme	organic	94:100	arg1	waste					102:106	organic waste	94:106	organic waste	94:106	isolated from organic waste and Kombucha.					
34044916	8	17	theme	phosphorus	888:897	arg1	rate					911:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate was 45 mg/L/day each.					
34044916	4	18	contain	has	446:448	arg2	homology					454:461	99% homology	450:461	99% homology with Komagataeibacter rhaeticus DSM 16663 T	450:505	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	4	18	contain	has	446:448	arg1	Strain					416:421	Strain	416:421	Strain isolated from Kombucha	416:444	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	4	19	attach	isolated	423:430	arg2	Strain					416:421	Strain	416:421	Strain isolated from Kombucha	416:444	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	4	19	attach	isolated	423:430	arg1	Kombucha					437:444	Kombucha	437:444	Kombucha	437:444	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	11	20	theme	composite	1310:1318	arg1	field					1290:1294	the field	1286:1294	the field of cosmetics, composite and wound care	1286:1333	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	7	21	theme	bacterial	829:837	arg1	nanocellulose					839:851	bacterial nanocellulose	829:851	bacterial nanocellulose	829:851	Maximum productivity of 4.5 g/L of bacterial nanocellulose was obtained.					
34044916	0	22	theme	production	34:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of nanocellulose production by strains of Komagataeibacter sp.	0:78	Characterization of nanocellulose production by strains of Komagataeibacter sp.					
34044916	6	23	theme	carbon	759:764	arg1	carbon					759:764	the utilized carbon	746:764	the utilized carbon converted to nanocellulose	746:791	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	6	23	theme	carbon	759:764	arg1	%					741:741	up to 69%	733:741	up to 69% of the utilized carbon converted to nanocellulose	733:791	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	11	24	theme	bacterial	1202:1210	arg1	cellulose					1212:1220	bacterial cellulose	1202:1220	bacterial cellulose	1202:1220	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	7	25	theme	nanocellulose	839:851	arg1	4.5 g/L					818:824	4.5 g/L	818:824	4.5 g/L of bacterial nanocellulose	818:851	Maximum productivity of 4.5 g/L of bacterial nanocellulose was obtained.					
34044916	4	26	with	homology	454:461	arg1	16663 T					499:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	0	27	theme	nanocellulose	20:32	arg1	production					34:43	nanocellulose production	20:43	nanocellulose production	20:43	Characterization of nanocellulose production by strains of Komagataeibacter sp.					
34044916	3	28	theme	fermented	304:312	arg1	tea					314:316	fermented tea	304:316	fermented tea	304:316	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	4	29	theme	Komagataeibacter	468:483	arg1	16663 T					499:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	7	30	theme	4.5 g/L	818:824	arg1	productivity					802:813	Maximum productivity	794:813	Maximum productivity of 4.5 g/L of bacterial nanocellulose	794:851	Maximum productivity of 4.5 g/L of bacterial nanocellulose was obtained.					
34044916	9	31	theme	transition	1010:1019	arg1	temperature					1021:1031	glass transition temperature	1004:1031	glass transition temperature	1004:1031	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	7	32	theme	Maximum	794:800	arg1	productivity					802:813	Maximum productivity	794:813	Maximum productivity of 4.5 g/L of bacterial nanocellulose	794:851	Maximum productivity of 4.5 g/L of bacterial nanocellulose was obtained.					
34044916	3	33	theme	organic	286:292	arg1	waste					294:298	organic waste	286:298	organic waste	286:298	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	11	34	with	materials	1184:1192	arg1	properties					1240:1249	their unique properties	1227:1249	their unique properties	1227:1249	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	6	35	with	concentration	713:725	arg1	carbon					759:764	the utilized carbon	746:764	the utilized carbon converted to nanocellulose	746:791	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	6	35	with	concentration	713:725	arg1	%					741:741	up to 69%	733:741	up to 69% of the utilized carbon converted to nanocellulose	733:791	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	4	36	theme	DSM	495:497	arg1	16663 T					499:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	11	37	theme	care	1330:1333	arg1	field					1290:1294	the field	1286:1294	the field of cosmetics, composite and wound care	1286:1333	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	5	38	theme	strain	574:579	arg1	productivity					553:564	nanocellulose productivity	539:564	nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose	539:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	8	39	theme	consumption	899:909	arg1	rate					911:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate	867:914	Average nitrogen and phosphorus consumption rate was 45 mg/L/day each.					
34044916	0	40	theme	Komagataeibacter	59:74	arg1	sp					76:77	Komagataeibacter sp	59:77	Komagataeibacter sp	59:77	Characterization of nanocellulose production by strains of Komagataeibacter sp.					
34044916	11	41	from	field	1290:1294	arg1	cellulose					1212:1220	bacterial cellulose	1202:1220	bacterial cellulose	1202:1220	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	11	41	from	field	1290:1294	arg1	materials					1184:1192	Renewable materials	1174:1192	Renewable materials such as bacterial cellulose with their unique properties	1174:1249	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	11	41	from	field	1290:1294	arg1	future					1259:1264	future	1259:1264	future	1259:1264	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	4	42	theme	rhaeticus	485:493	arg1	16663 T					499:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Komagataeibacter rhaeticus DSM 16663 T	468:505	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	4	43	theme	99	450:451	arg1	%					452:452	%	452:452	%	452:452	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	2	44	theme	medical	228:234	arg1	industry					236:243	medical industry	228:243	medical industry	228:243	Bacterial nanocellulose production is gaining popularity owing to its applications in food, cosmetics and medical industry.					
34044916	5	45	theme	different	586:594	arg1	glycerol					628:635	glycerol	628:635	glycerol	628:635	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	45	theme	different	586:594	arg1	sources					603:609	different carbon sources	586:609	different carbon sources such as glucose, glycerol, fructose and sucrose	586:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	45	theme	different	586:594	arg1	glucose					619:625	glucose	619:625	glucose	619:625	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	45	theme	different	586:594	arg1	sucrose					651:657	sucrose	651:657	sucrose	651:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	45	theme	different	586:594	arg1	fructose					638:645	fructose	638:645	fructose	638:645	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	10	46	theme	Bacterial	1047:1055	arg1	cellulose					1057:1065	Bacterial cellulose	1047:1065	Bacterial cellulose	1047:1065	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	6	47	theme	%	678:678	arg1	glycerol					680:687	1% glycerol	677:687	1% glycerol	677:687	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	5	48	theme	carbon	596:601	arg1	glycerol					628:635	glycerol	628:635	glycerol	628:635	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	48	theme	carbon	596:601	arg1	sources					603:609	different carbon sources	586:609	different carbon sources such as glucose, glycerol, fructose and sucrose	586:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	48	theme	carbon	596:601	arg1	glucose					619:625	glucose	619:625	glucose	619:625	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	48	theme	carbon	596:601	arg1	sucrose					651:657	sucrose	651:657	sucrose	651:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	48	theme	carbon	596:601	arg1	fructose					638:645	fructose	638:645	fructose	638:645	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	6	49	theme	1	677:677	arg1	%					678:678	%	678:678	%	678:678	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	10	50	used	used	1118:1121	arg2	source					1133:1138	carbon source	1126:1138	carbon source	1126:1138	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	10	50	used	used	1118:1121	arg2	glycerol					1092:1099	glycerol	1092:1099	glycerol	1092:1099	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	10	50	used	used	1118:1121	arg2	%					1146:1146	73%	1144:1146	73% for fructose and sucrose	1144:1171	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	10	50	used	used	1118:1121	arg2	glucose					1105:1111	glucose	1105:1111	glucose	1105:1111	Bacterial cellulose was 80% crystalline when glycerol and glucose were used as carbon source and 73% for fructose and sucrose.					
34044916	6	51	theme	utilized	750:757	arg1	carbon					759:764	the utilized carbon	746:764	the utilized carbon converted to nanocellulose	746:791	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	5	52	theme	nanocellulose	539:551	arg1	productivity					553:564	nanocellulose productivity	539:564	nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose	539:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	6	53	dep	69	739:740	arg1	to					736:737	to	736:737	to	736:737	1% glycerol was found to be optimal concentration, with up to 69% of the utilized carbon converted to nanocellulose.					
34044916	3	54	attach	isolated	272:279	arg2	strains					264:270	Three Acetobacter strains	246:270	Three Acetobacter strains isolated from organic waste and fermented tea	246:316	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	3	54	attach	isolated	272:279	arg1	waste					294:298	organic waste	286:298	organic waste	286:298	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	3	54	attach	isolated	272:279	arg1	tea					314:316	fermented tea	304:316	fermented tea	304:316	Three Acetobacter strains isolated from organic waste and fermented tea were identified using 16S rDNA sequencing and their ability to produce nanocellulose was studied.					
34044916	4	55	theme	%	452:452	arg1	homology					454:461	99% homology	450:461	99% homology with Komagataeibacter rhaeticus DSM 16663 T	450:505	Strain isolated from Kombucha has 99% homology with Komagataeibacter rhaeticus DSM 16663 T.					
34044916	0	56	theme	sp	76:77	arg1	strains					48:54	strains	48:54	strains of Komagataeibacter sp	48:77	Characterization of nanocellulose production by strains of Komagataeibacter sp.					
34044916	9	57	theme	glass	1004:1008	arg1	temperature					1021:1031	glass transition temperature	1004:1031	glass transition temperature	1004:1031	Physical properties such as crystallinity, fibril dimensions, and glass transition temperature were studied.					
34044916	11	58	theme	unique	1233:1238	arg1	properties					1240:1249	their unique properties	1227:1249	their unique properties	1227:1249	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	11	59	from	future	1259:1264	arg1	field					1290:1294	the field	1286:1294	the field of cosmetics, composite and wound care	1286:1333	Renewable materials such as bacterial cellulose with their unique properties are the future for applications in the field of cosmetics, composite and wound care.					
34044916	5	60	with	productivity	553:564	arg1	glycerol					628:635	glycerol	628:635	glycerol	628:635	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	60	with	productivity	553:564	arg1	sources					603:609	different carbon sources	586:609	different carbon sources such as glucose, glycerol, fructose and sucrose	586:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	60	with	productivity	553:564	arg1	glucose					619:625	glucose	619:625	glucose	619:625	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	60	with	productivity	553:564	arg1	sucrose					651:657	sucrose	651:657	sucrose	651:657	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34044916	5	60	with	productivity	553:564	arg1	fructose					638:645	fructose	638:645	fructose	638:645	This is the first report where nanocellulose productivity of this strain with different carbon sources such as glucose, glycerol, fructose and sucrose has been studied.					
34737079	0	0	from	characterization	35:50	arg1	HDX-1					137:141	Gluconobacter cerinus HDX-1	115:141	Gluconobacter cerinus HDX-1	115:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	4	1	theme	tensile	998:1004	arg1	strength					1006:1013	tensile strength	998:1013	tensile strength	998:1013	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	3	2	theme	spectrometer	693:704	arg1	form					648:651	typical crystalline form	628:651	typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS)	628:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	3	theme	electron	722:729	arg1	SEM					743:745	SEM	743:745	SEM	743:745	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	3	theme	electron	722:729	arg1	microscope					731:740	scanning electron microscope	713:740	scanning electron microscope (SEM)	713:746	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	4	theme	diffraction	562:572	arg1	analysis					580:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis	458:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis	458:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	5	theme	complexation	275:286	arg1	method					288:293	a simple, low-cost complexation method	256:293	a simple, low-cost complexation method	256:293	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	1	6	used	used	299:302	arg2	method					288:293	a simple, low-cost complexation method	256:293	a simple, low-cost complexation method	256:293	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	3	7	theme	transmission	968:979	arg1	rate					981:984	water vapor transmission rate	956:984	water vapor transmission rate (WVTR)	956:991	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	7	theme	transmission	968:979	arg1	WVTR					987:990	WVTR	987:990	WVTR	987:990	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	8	theme	-producing	184:193	arg1	HDX-1					217:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	1	9	theme	cerinus	209:215	arg1	HDX-1					217:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	4	10	theme	nanofibers	1043:1052	arg1	modulus					1027:1033	Young's modulus	1019:1033	Young's modulus	1019:1033	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	4	10	theme	nanofibers	1043:1052	arg1	strength					1006:1013	tensile strength	998:1013	tensile strength	998:1013	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	4	11	dep	nanofiber	1136:1144	arg1	101.59 ± 5.87 MPa					1167:1183	101.59 ± 5.87 MPa	1167:1183	101.59 ± 5.87 MPa	1167:1183	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	4	11	dep	nanofiber	1136:1144	arg1	6.12 ± 0.87 MPa					1147:1161	6.12 ± 0.87 MPa	1147:1161	6.12 ± 0.87 MPa	1147:1161	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	4	12	theme	BC	1133:1134	arg1	nanofiber					1136:1144	BC nanofiber	1133:1144	BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa)	1133:1184	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	3	13	theme	atomic	752:757	arg1	AFM					777:779	AFM	777:779	AFM	777:779	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	13	theme	atomic	752:757	arg1	microscopy					765:774	atomic force microscopy	752:774	atomic force microscopy (AFM)	752:780	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	14	theme	new	146:148	arg1	strain					150:155	A new strain	144:155	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	144:221	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	0	15	from	HDX-1	137:141	arg1	biosynthesis					18:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	0	15	from	HDX-1	137:141	arg1	nanofiber					100:108	novel bacteriocin-loaded bacterial cellulose nanofiber	55:108	novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1	55:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	0	15	from	HDX-1	137:141	arg1	characterization					35:50	characterization	35:50	characterization	35:50	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	3	16	theme	typical	628:634	arg1	form					648:651	typical crystalline form	628:651	typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS)	628:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	0	17	theme	bacterial	80:88	arg1	nanofiber					100:108	novel bacteriocin-loaded bacterial cellulose nanofiber	55:108	novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1	55:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	1	18	theme	bacteriocin	348:358	arg1	nanofiber					370:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	0	19	from	biosynthesis	18:29	arg1	HDX-1					137:141	Gluconobacter cerinus HDX-1	115:141	Gluconobacter cerinus HDX-1	115:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	0	20	theme	nanofiber	100:108	arg1	biosynthesis					18:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	0	20	theme	nanofiber	100:108	arg1	characterization					35:50	characterization	35:50	characterization	35:50	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	0	21	theme	Gluconobacter	115:127	arg1	HDX-1					137:141	Gluconobacter cerinus HDX-1	115:141	Gluconobacter cerinus HDX-1	115:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	4	22	dep	strength	1006:1013	arg1	The					994:996	The	994:996	The	994:996	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	5	23	theme	significant	1309:1319	arg1	stability					1329:1337	significant thermal stability	1309:1337	significant thermal stability	1309:1337	Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber.					
34737079	3	24	theme	Solid-state	458:468	arg1	resonance					487:495	Solid-state nuclear magnetic resonance	458:495	Solid-state nuclear magnetic resonance (NMR)	458:501	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	24	theme	Solid-state	458:468	arg1	NMR					498:500	NMR	498:500	NMR	498:500	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	24	theme	Solid-state	458:468	arg1	XRD					575:577	XRD	575:577	XRD	575:577	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	25	theme	BC-B	870:873	arg1	nanofiber					875:883	BC-B nanofiber	870:883	BC-B nanofiber	870:883	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	26	dep	Lactobacillus	320:332	arg1	paracasei					334:342	paracasei	334:342	paracasei	334:342	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	0	27	theme	Ecofriendly	0:10	arg1	biosynthesis					18:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	6	28	theme	bacteriocin-loaded	1418:1435	arg1	nanofiber					1440:1448	bacteriocin-loaded BC nanofiber	1418:1448	bacteriocin-loaded BC nanofiber	1418:1448	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	6	28	theme	bacteriocin-loaded	1418:1435	arg1	agents					1479:1484	antimicrobial agents	1465:1484	antimicrobial agents in active food packaging and medical material	1465:1530	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	4	29	theme	BC	1252:1253	arg1	nanofiber					1255:1263	BC nanofiber	1252:1263	BC nanofiber	1252:1263	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	3	30	theme	magnetic	478:485	arg1	resonance					487:495	Solid-state nuclear magnetic resonance	458:495	Solid-state nuclear magnetic resonance (NMR)	458:501	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	30	theme	magnetic	478:485	arg1	NMR					498:500	NMR	498:500	NMR	498:500	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	30	theme	magnetic	478:485	arg1	XRD					575:577	XRD	575:577	XRD	575:577	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	31	theme	biosynthesis	307:318	arg1	nanofiber					370:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	6	32	from	agents	1479:1484	arg1	packaging					1501:1509	active food packaging	1489:1509	active food packaging	1489:1509	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	6	32	from	agents	1479:1484	arg1	material					1523:1530	medical material	1515:1530	medical material	1515:1530	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	3	33	theme	Fourier	504:510	arg1	analysis					580:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis	458:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis	458:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	0	34	theme	novel	55:59	arg1	nanofiber					100:108	novel bacteriocin-loaded bacterial cellulose nanofiber	55:108	novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1	55:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	3	35	theme	photoelectron	679:691	arg1	XPS					707:709	XPS	707:709	XPS	707:709	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	35	theme	photoelectron	679:691	arg1	spectrometer					693:704	the cellulose I. X-ray photoelectron spectrometer	656:704	the cellulose I. X-ray photoelectron spectrometer (XPS)	656:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	5	36	theme	BC	1393:1394	arg1	nanofiber					1396:1404	BC nanofiber	1393:1404	BC nanofiber	1393:1404	Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber.					
34737079	6	37	theme	active	1489:1494	arg1	packaging					1501:1509	active food packaging	1489:1509	active food packaging	1489:1509	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	4	38	theme	BC-B	1038:1041	arg1	nanofibers					1043:1052	BC-B nanofibers	1038:1052	BC-B nanofibers	1038:1052	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	3	39	theme	I.	670:671	arg1	XPS					707:709	XPS	707:709	XPS	707:709	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	39	theme	I.	670:671	arg1	spectrometer					693:704	the cellulose I. X-ray photoelectron spectrometer	656:704	the cellulose I. X-ray photoelectron spectrometer (XPS)	656:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	5	40	theme	antioxidant	1340:1350	arg1	capacity					1352:1359	antioxidant capacity	1340:1359	antioxidant capacity	1340:1359	Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber.					
34737079	3	41	contain	had	624:626	arg1	nanofibers					613:622	BC and BC-B nanofibers	601:622	nanofibers	613:622	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	41	contain	had	624:626	arg2	form					648:651	typical crystalline form	628:651	typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS)	628:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	42	theme	infrared	522:529	arg1	spectroscopy					531:542	infrared spectroscopy	522:542	infrared spectroscopy (FT-IR)	522:550	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	42	theme	infrared	522:529	arg1	FT-IR					545:549	FT-IR	545:549	FT-IR	545:549	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	43	theme	simple	258:263	arg1	method					288:293	a simple, low-cost complexation method	256:293	a simple, low-cost complexation method	256:293	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	4	44	theme	Young	1019:1023	arg1	modulus					1027:1033	Young's modulus	1019:1033	Young's modulus	1019:1033	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	3	45	theme	x-ray	556:560	arg1	diffraction					562:572	x-ray diffraction	556:572	x-ray diffraction	556:572	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	46	theme	vapor	962:966	arg1	rate					981:984	water vapor transmission rate	956:984	water vapor transmission rate (WVTR)	956:991	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	46	theme	vapor	962:966	arg1	WVTR					987:990	WVTR	987:990	WVTR	987:990	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	47	theme	scanning	713:720	arg1	SEM					743:745	SEM	743:745	SEM	743:745	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	47	theme	scanning	713:720	arg1	microscope					731:740	scanning electron microscope	713:740	scanning electron microscope (SEM)	713:746	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	4	48	dep	higher	1113:1118	arg1	13.28 ± 1.26 MPa					1059:1074	13.28 ± 1.26 MPa	1059:1074	13.28 ± 1.26 MPa	1059:1074	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	4	48	dep	higher	1113:1118	arg1	132.10 ± 4.92 MPa					1080:1096	132.10 ± 4.92 MPa	1080:1096	132.10 ± 4.92 MPa	1080:1096	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	1	49	theme	Gluconobacter	195:207	arg1	HDX-1					217:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	3	50	theme	X-ray	673:677	arg1	XPS					707:709	XPS	707:709	XPS	707:709	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	50	theme	X-ray	673:677	arg1	spectrometer					693:704	the cellulose I. X-ray photoelectron spectrometer	656:704	the cellulose I. X-ray photoelectron spectrometer (XPS)	656:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	6	51	theme	medical	1515:1521	arg1	material					1523:1530	medical material	1515:1530	medical material	1515:1530	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	1	52	theme	HDX-1	217:221	arg1	strain					150:155	A new strain	144:155	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	144:221	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	3	53	theme	BC	601:602	arg1	nanofibers					613:622	BC and BC-B nanofibers	601:622	nanofibers	613:622	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	6	54	used	used	1457:1460	arg2	nanofiber					1440:1448	bacteriocin-loaded BC nanofiber	1418:1448	bacteriocin-loaded BC nanofiber	1418:1448	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	6	54	used	used	1457:1460	arg2	agents					1479:1484	antimicrobial agents	1465:1484	antimicrobial agents in active food packaging and medical material	1465:1530	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	3	55	theme	force	759:763	arg1	AFM					777:779	AFM	777:779	AFM	777:779	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	55	theme	force	759:763	arg1	microscopy					765:774	atomic force microscopy	752:774	atomic force microscopy (AFM)	752:780	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	56	dep	simple	258:263	arg1	low-cost					266:273	low-cost	266:273	low-cost	266:273	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	0	57	theme	cellulose	90:98	arg1	nanofiber					100:108	novel bacteriocin-loaded bacterial cellulose nanofiber	55:108	novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1	55:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	3	58	theme	BC-B	608:611	arg1	nanofibers					613:622	BC and BC-B nanofibers	601:622	nanofibers	613:622	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	1	59	theme	bacterial	160:168	arg1	HDX-1					217:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1	160:221	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	1	60	theme	BC	360:361	arg1	nanofiber					370:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	0	61	theme	cerinus	129:135	arg1	HDX-1					137:141	Gluconobacter cerinus HDX-1	115:141	Gluconobacter cerinus HDX-1	115:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	1	62	theme	BC-B	364:367	arg1	nanofiber					370:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	3	63	dep	Fourier	504:510	arg1	transform					512:520	transform	512:520	transform infrared spectroscopy (FT-IR)	512:550	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	5	64	theme	thermal	1321:1327	arg1	stability					1329:1337	significant thermal stability	1309:1337	significant thermal stability	1309:1337	Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber.					
34737079	3	65	theme	nuclear	470:476	arg1	resonance					487:495	Solid-state nuclear magnetic resonance	458:495	Solid-state nuclear magnetic resonance (NMR)	458:501	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	65	theme	nuclear	470:476	arg1	NMR					498:500	NMR	498:500	NMR	498:500	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	65	theme	nuclear	470:476	arg1	XRD					575:577	XRD	575:577	XRD	575:577	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	0	66	theme	green	12:16	arg1	biosynthesis					18:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis	0:29	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	3	67	theme	nanofiber	875:883	arg1	tighter					889:895	tighter	889:895	tighter	889:895	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	67	theme	nanofiber	875:883	arg1	structure					857:865	the structure	853:865	the structure of BC-B nanofiber	853:883	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	6	68	theme	BC	1437:1438	arg1	nanofiber					1440:1448	bacteriocin-loaded BC nanofiber	1418:1448	bacteriocin-loaded BC nanofiber	1418:1448	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	6	68	theme	BC	1437:1438	arg1	agents					1479:1484	antimicrobial agents	1465:1484	antimicrobial agents in active food packaging and medical material	1465:1530	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	4	69	theme	nanofiber	1255:1263	arg1	properties					1238:1247	the mechanical properties	1223:1247	the mechanical properties of BC nanofiber	1223:1263	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	5	70	theme	BC-B	1283:1286	arg1	nanofibers					1288:1297	the BC-B nanofibers	1279:1297	the BC-B nanofibers	1279:1297	Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber.					
34737079	2	71	theme	nanofibers	431:440	arg1	structure					385:393	structure	385:393	structure	385:393	The structure and antibacterial properties of the nanofibers were evaluated.					
34737079	2	71	theme	nanofibers	431:440	arg1	properties					413:422	antibacterial properties	399:422	antibacterial properties	399:422	The structure and antibacterial properties of the nanofibers were evaluated.					
34737079	3	72	theme	BC	902:903	arg1	nanofiber					905:913	BC nanofiber	902:913	BC nanofiber	902:913	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	73	theme	crystalline	636:646	arg1	form					648:651	typical crystalline form	628:651	typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS)	628:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	5	74	theme	antibacterial	1365:1377	arg1	activity					1379:1386	antibacterial activity	1365:1386	antibacterial activity	1365:1386	Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber.					
34737079	3	75	theme	resonance	487:495	arg1	analysis					580:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis	458:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis	458:587	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	0	76	theme	bacteriocin-loaded	61:78	arg1	nanofiber					100:108	novel bacteriocin-loaded bacterial cellulose nanofiber	55:108	novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1	55:141	Ecofriendly green biosynthesis and characterization of novel bacteriocin-loaded bacterial cellulose nanofiber from Gluconobacter cerinus HDX-1.					
34737079	1	77	theme	Lactobacillus	320:332	arg1	nanofiber					370:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	4	78	theme	mechanical	1227:1236	arg1	properties					1238:1247	the mechanical properties	1223:1247	the mechanical properties of BC nanofiber	1223:1263	The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber.					
34737079	6	79	theme	food	1496:1499	arg1	packaging					1501:1509	active food packaging	1489:1509	active food packaging	1489:1509	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	1	80	theme	1∙7	344:346	arg1	nanofiber					370:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber	307:378	A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber.					
34737079	3	81	theme	lower	921:925	arg1	porosity					927:934	lower porosity	921:934	lower porosity	921:934	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	82	theme	cellulose	660:668	arg1	XPS					707:709	XPS	707:709	XPS	707:709	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	82	theme	cellulose	660:668	arg1	spectrometer					693:704	the cellulose I. X-ray photoelectron spectrometer	656:704	the cellulose I. X-ray photoelectron spectrometer (XPS)	656:710	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	2	83	dep	structure	385:393	arg1	The					381:383	The	381:383	The	381:383	The structure and antibacterial properties of the nanofibers were evaluated.					
34737079	3	84	theme	water	956:960	arg1	rate					981:984	water vapor transmission rate	956:984	water vapor transmission rate (WVTR)	956:991	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	3	84	theme	water	956:960	arg1	WVTR					987:990	WVTR	987:990	WVTR	987:990	Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR).					
34737079	2	85	theme	antibacterial	399:411	arg1	properties					413:422	antibacterial properties	399:422	antibacterial properties	399:422	The structure and antibacterial properties of the nanofibers were evaluated.					
34737079	6	86	theme	antimicrobial	1465:1477	arg1	nanofiber					1440:1448	bacteriocin-loaded BC nanofiber	1418:1448	bacteriocin-loaded BC nanofiber	1418:1448	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
34737079	6	86	theme	antimicrobial	1465:1477	arg1	agents					1479:1484	antimicrobial agents	1465:1484	antimicrobial agents in active food packaging and medical material	1465:1530	Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.					
33666538	10	0	theme	APOE-dependent	1792:1805	arg1	effects					1820:1826	an APOE-dependent nutrigenetic effects	1789:1826	an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection	1789:1880	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	1	1	theme	prebiotic	256:264	arg1	inulin					246:251	inulin	246:251	inulin	246:251	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	1	1	theme	prebiotic	256:264	arg1	fiber					266:270	a prebiotic fiber	254:270	a prebiotic fiber	254:270	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	4	2	with	mice	651:654	arg1	inulin					668:673	either inulin or control diet	661:689	inulin	668:673	We fed mice with either inulin or control diet for 16 weeks starting from 3 months of age.					
33666538	4	2	with	mice	651:654	arg1	diet					686:689	either inulin or control diet	661:689	diet	686:689	We fed mice with either inulin or control diet for 16 weeks starting from 3 months of age.					
33666538	7	3	theme	bile	1329:1332	arg1	acids					1334:1338	bile acids	1329:1338	bile acids	1329:1338	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	9	4	dep	gut	1568:1570	arg1	the					1564:1566	the	1564:1566	the	1564:1566	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	1	5	theme	study	186:190	arg1	goal					174:177	The goal	170:177	The goal of the study	170:190	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	5	6	theme	systemic	816:823	arg1	metabolism					825:834	systemic metabolism	816:834	systemic metabolism using in vivo MRI and metabolomics	816:869	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	1	7	with	mice	276:279	arg1	variants					334:341	different human apolipoprotein E (APOE) genetic variants	286:341	different human apolipoprotein E (APOE) genetic variants	286:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	7	8	theme	phosphate	1306:1314	arg1	pathway					1316:1322	pentose phosphate pathway	1298:1322	pentose phosphate pathway	1298:1322	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	7	9	theme	E3FAD	1166:1170	arg1	mice					1172:1175	E3FAD mice	1166:1175	E3FAD mice	1166:1175	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	3	10	dep	APOE3	597:601	arg1	mice					610:613	E3FAD mice	604:613	E3FAD mice	604:613	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	3	10	dep	APOE3	597:601	arg1	mice					637:640	APOE4 gene (E4FAD mice	619:640	APOE4 gene (E4FAD mice	619:640	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	2	11	from	axis	480:483	arg1	mice					488:491	mice	488:491	mice with human APOE ϵ3 and ϵ4 alleles	488:525	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	9	12	dep	had	1525:1527	arg1	reduce					1661:1666	reduce	1661:1666	reduce risk for neurodegeneration	1661:1693	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	9	12	dep	had	1525:1527	arg1	protective					1626:1635	protective	1626:1635	protective	1626:1635	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	5	13	theme	gut	749:751	arg1	diversity					764:772	gut microbiome diversity	749:772	gut microbiome diversity	749:772	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	5	14	theme	using16s	790:797	arg1	sequencing					804:813	using16s rRNA sequencing	790:813	using16s rRNA sequencing	790:813	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	7	15	theme	acid	1286:1289	arg1	cycle					1291:1295	the tricarboxylic acid cycle	1268:1295	the tricarboxylic acid cycle	1268:1295	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	9	16	contain	had	1525:1527	arg2	effects					1553:1559	many similar beneficial effects	1529:1559	many similar beneficial effects	1529:1559	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	9	16	contain	had	1525:1527	arg1	inulin					1518:1523	inulin	1518:1523	inulin	1518:1523	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	6	17	theme	fatty	1122:1126	arg1	acid					1128:1131	cecal short chain fatty acid	1104:1131	cecal short chain fatty acid	1104:1131	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	7	18	contain	had	1253:1255	arg2	changes					1257:1263	changes	1257:1263	changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids	1257:1338	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	7	18	contain	had	1253:1255	arg1	mice					1248:1251	E4FAD mice	1242:1251	E4FAD mice	1242:1251	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	3	19	theme	E3FAD	604:608	arg1	mice					610:613	E3FAD mice	604:613	E3FAD mice	604:613	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	3	20	dep	METHOD	528:533	arg1	performed					538:546	performed	538:546	performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice)	538:641	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	8	21	theme	metabolites	1383:1393	arg1	levels					1428:1433	levels	1428:1433	levels of Claudin-1 and Claudin-5 BBB tight junction expression	1428:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	8	21	theme	metabolites	1383:1393	arg1	levels					1367:1372	levels	1367:1372	levels of brain metabolites related to oxidative stress	1367:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	6	22	theme	short	1110:1114	arg1	acid					1128:1131	cecal short chain fatty acid	1104:1131	cecal short chain fatty acid	1104:1131	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	10	23	theme	E4FAD	1708:1712	arg1	mice					1714:1717	E3FAD and E4FAD mice	1698:1717	E3FAD and E4FAD mice	1698:1717	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	9	24	theme	brain	1641:1645	arg1	functions					1647:1655	brain functions	1641:1655	brain functions	1641:1655	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	0	25	theme	systemic	92:99	arg1	metabolism					101:110	systemic metabolism	92:110	systemic metabolism	92:110	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	1	26	theme	genetic	326:332	arg1	variants					334:341	different human apolipoprotein E (APOE) genetic variants	286:341	different human apolipoprotein E (APOE) genetic variants	286:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	8	27	theme	related	1395:1401	arg1	metabolites					1383:1393	brain metabolites	1377:1393	brain metabolites related to oxidative stress	1377:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	10	28	theme	several	1750:1756	arg1	pathways					1768:1775	several metabolic pathways	1750:1775	several metabolic pathways	1750:1775	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	7	29	theme	tricarboxylic	1272:1284	arg1	cycle					1291:1295	the tricarboxylic acid cycle	1268:1295	the tricarboxylic acid cycle	1268:1295	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	10	30	theme	E3FAD	1698:1702	arg1	mice					1714:1717	E3FAD and E4FAD mice	1698:1717	E3FAD and E4FAD mice	1698:1717	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	7	31	from	changes	1257:1263	arg1	cycle					1291:1295	the tricarboxylic acid cycle	1268:1295	the tricarboxylic acid cycle	1268:1295	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	7	31	from	changes	1257:1263	arg1	pathway					1316:1322	pentose phosphate pathway	1298:1322	pentose phosphate pathway	1298:1322	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	7	31	from	changes	1257:1263	arg1	acids					1334:1338	bile acids	1329:1338	bile acids	1329:1338	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	2	32	theme	APOE	504:507	arg1	ϵ3					509:510	human APOE ϵ3 and ϵ4 alleles	498:525	ϵ3	509:510	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	5	33	theme	junction	908:915	arg1	expression					917:926	tight junction expression	902:926	tight junction expression	902:926	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	2	34	theme	metabolism	435:444	arg1	modulation					408:417	the potential modulation	394:417	the potential modulation of the systemic metabolism and neuroprotection	394:464	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	5	35	dep	in	842:843	arg1	vivo					845:848	vivo	845:848	vivo	845:848	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	10	36	theme	systemic	1842:1849	arg1	metabolism					1851:1860	systemic metabolism	1842:1860	systemic metabolism	1842:1860	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	0	37	theme	Apolipoprotein	0:13	arg1	E					15:15	Apolipoprotein E	0:15	Apolipoprotein E	0:15	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	5	38	theme	blood-brain	876:886	arg1	barrier					888:894	blood-brain barrier	876:894	blood-brain barrier (BBB)	876:900	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	5	38	theme	blood-brain	876:886	arg1	BBB					897:899	BBB	897:899	BBB	897:899	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	7	39	theme	E4FAD	1242:1246	arg1	mice					1248:1251	E4FAD mice	1242:1251	E4FAD mice	1242:1251	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	1	40	dep	OBJECTIVE	160:168	arg1	was					192:194	was	192:194	was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants	192:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	5	41	theme	microbiome	753:762	arg1	diversity					764:772	gut microbiome diversity	749:772	gut microbiome diversity	749:772	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	6	42	theme	bacteria	1082:1089	arg1	taxa					1074:1077	beneficial taxa	1063:1077	beneficial taxa of bacteria	1063:1089	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	2	43	theme	gut-brain	470:478	arg1	axis					480:483	gut-brain axis	470:483	gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles	470:525	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	8	44	theme	BBB	1462:1464	arg1	expression					1481:1490	Claudin-5 BBB tight junction expression	1452:1490	Claudin-5 BBB tight junction expression	1452:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	3	45	theme	gene	625:628	arg1	mice					637:640	APOE4 gene (E4FAD mice	619:640	APOE4 gene (E4FAD mice	619:640	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	1	46	theme	human	296:300	arg1	APOE					320:323	APOE	320:323	APOE	320:323	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	1	46	theme	human	296:300	arg1	E					317:317	human apolipoprotein E	296:317	different human apolipoprotein E (APOE) genetic variants	286:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	2	47	theme	neuroprotection	450:464	arg1	modulation					408:417	the potential modulation	394:417	the potential modulation of the systemic metabolism and neuroprotection	394:464	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	8	48	theme	junction	1472:1479	arg1	expression					1481:1490	Claudin-5 BBB tight junction expression	1452:1490	Claudin-5 BBB tight junction expression	1452:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	10	49	theme	distinct	1728:1735	arg1	responses					1737:1745	distinct responses	1728:1745	distinct responses	1728:1745	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	0	50	theme	prebiotic	60:68	arg1	inulin					70:75	prebiotic inulin	60:75	prebiotic inulin	60:75	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	1	51	theme	E	317:317	arg1	variants					334:341	different human apolipoprotein E (APOE) genetic variants	286:341	different human apolipoprotein E (APOE) genetic variants	286:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	6	52	theme	gut	1037:1039	arg1	microbiome					1041:1050	the gut microbiome	1033:1050	the gut microbiome	1033:1050	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	9	53	theme	many	1529:1532	arg1	effects					1553:1559	many similar beneficial effects	1529:1559	many similar beneficial effects	1529:1559	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	3	54	theme	E4FAD	631:635	arg1	mice					637:640	APOE4 gene (E4FAD mice	619:640	APOE4 gene (E4FAD mice	619:640	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	5	55	dep	diversity	764:772	arg1	sequencing					804:813	using16s rRNA sequencing	790:813	using16s rRNA sequencing	790:813	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	5	55	dep	diversity	764:772	arg1	expression					917:926	tight junction expression	902:926	tight junction expression	902:926	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	9	56	theme	beneficial	1542:1551	arg1	effects					1553:1559	many similar beneficial effects	1529:1559	many similar beneficial effects	1529:1559	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	6	57	theme	beta	1015:1018	arg1	diversity					1020:1028	the alpha and beta diversity	1001:1028	diversity	1020:1028	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	10	58	theme	nutrigenetic	1807:1818	arg1	effects					1820:1826	an APOE-dependent nutrigenetic effects	1789:1826	an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection	1789:1880	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	6	59	theme	alpha	1005:1009	arg1	diversity					1020:1028	the alpha and beta diversity	1001:1028	diversity	1020:1028	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	6	60	theme	microbiome	1041:1050	arg1	diversity					1020:1028	the alpha and beta diversity	1001:1028	diversity	1020:1028	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	5	61	theme	rRNA	799:802	arg1	sequencing					804:813	using16s rRNA sequencing	790:813	using16s rRNA sequencing	790:813	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	7	62	contain	had	1177:1179	arg1	mice					1172:1175	E3FAD mice	1166:1175	E3FAD mice	1166:1175	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	7	62	contain	had	1177:1179	arg2	metabolism					1189:1198	altered metabolism	1181:1198	altered metabolism related to tryptophan and tyrosine	1181:1233	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	6	63	theme	beneficial	1063:1072	arg1	taxa					1074:1077	beneficial taxa	1063:1077	beneficial taxa of bacteria	1063:1089	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	0	64	theme	gut-brain	144:152	arg1	axis					154:157	gut-brain axis	144:157	gut-brain axis	144:157	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	0	65	from	metabolism	101:110	arg1	mice					135:138	mice	135:138	mice	135:138	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	1	66	theme	potential	212:220	arg1	effects					235:241	the potential nutrigenetic effects	208:241	the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants	208:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	2	67	theme	ϵ4	516:517	arg1	alleles					519:525	human APOE ϵ3 and ϵ4 alleles	498:525	alleles	519:525	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	7	68	theme	pentose	1298:1304	arg1	pathway					1316:1322	pentose phosphate pathway	1298:1322	pentose phosphate pathway	1298:1322	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	3	69	theme	young	565:569	arg1	mice					571:574	young mice	565:574	young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice)	565:641	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	8	70	theme	brain	1377:1381	arg1	metabolites					1383:1393	brain metabolites	1377:1393	brain metabolites related to oxidative stress	1377:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	3	71	with	experiments	548:558	arg1	mice					571:574	young mice	565:574	young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice)	565:641	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	1	72	from	effects	235:241	arg1	mice					276:279	mice	276:279	mice with different human apolipoprotein E (APOE) genetic variants	276:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	8	73	located	found	1358:1362	arg2	Differences					1341:1351	Differences	1341:1351	Differences	1341:1351	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	8	73	located	found	1358:1362	arg1	levels					1428:1433	levels	1428:1433	levels of Claudin-1 and Claudin-5 BBB tight junction expression	1428:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	8	73	located	found	1358:1362	arg1	levels					1367:1372	levels	1367:1372	levels of brain metabolites related to oxidative stress	1367:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	0	74	from	neuroprotection	116:130	arg1	mice					135:138	mice	135:138	mice	135:138	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	6	75	theme	chain	1116:1120	arg1	acid					1128:1131	cecal short chain fatty acid	1104:1131	cecal short chain fatty acid	1104:1131	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	3	76	theme	human	591:595	arg1	APOE3					597:601	the human APOE3	587:601	the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice)	587:641	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	8	77	theme	oxidative	1406:1414	arg1	stress					1416:1421	oxidative stress	1406:1421	oxidative stress	1406:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	6	78	theme	cecal	1104:1108	arg1	acid					1128:1131	cecal short chain fatty acid	1104:1131	cecal short chain fatty acid	1104:1131	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	9	79	dep	DISCUSSION	1493:1502	arg1	found					1507:1511	found	1507:1511	found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration	1507:1693	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	9	80	dep	E3FAD	1591:1595	arg1	mice					1607:1610	mice	1607:1610	mice	1607:1610	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	5	81	theme	in	842:843	arg1	MRI					850:852	in vivo MRI	842:852	in vivo MRI	842:852	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	6	82	dep	RESULTS	948:954	arg1	altered					993:999	altered	993:999	altered the alpha and beta diversity of the gut microbiome	993:1050	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	6	82	dep	RESULTS	948:954	arg1	increased					1053:1061	increased	1053:1061	increased beneficial taxa of bacteria	1053:1089	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	6	82	dep	RESULTS	948:954	arg1	elevated					1095:1102	elevated	1095:1102	elevated cecal short chain fatty acid and hippocampal scyllo-inositol	1095:1163	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	6	83	dep	E3FAD	964:968	arg1	mice					980:983	mice	980:983	mice	980:983	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	2	84	with	mice	488:491	arg1	ϵ3					509:510	human APOE ϵ3 and ϵ4 alleles	498:525	ϵ3	509:510	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	2	84	with	mice	488:491	arg1	alleles					519:525	human APOE ϵ3 and ϵ4 alleles	498:525	alleles	519:525	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	5	85	theme	tight	902:906	arg1	expression					917:926	tight junction expression	902:926	tight junction expression	902:926	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	2	86	theme	human	498:502	arg1	ϵ3					509:510	human APOE ϵ3 and ϵ4 alleles	498:525	ϵ3	509:510	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	8	87	theme	Claudin-1	1438:1446	arg1	levels					1428:1433	levels	1428:1433	levels of Claudin-1 and Claudin-5 BBB tight junction expression	1428:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	8	87	theme	Claudin-1	1438:1446	arg1	levels					1367:1372	levels	1367:1372	levels of brain metabolites related to oxidative stress	1367:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	2	88	theme	systemic	426:433	arg1	metabolism					435:444	the systemic metabolism	422:444	the systemic metabolism	422:444	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	8	89	theme	Claudin-5	1452:1460	arg1	expression					1481:1490	Claudin-5 BBB tight junction expression	1452:1490	Claudin-5 BBB tight junction expression	1452:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	10	90	theme	metabolic	1758:1766	arg1	pathways					1768:1775	several metabolic pathways	1750:1775	several metabolic pathways	1750:1775	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	4	91	theme	control	678:684	arg1	diet					686:689	either inulin or control diet	661:689	diet	686:689	We fed mice with either inulin or control diet for 16 weeks starting from 3 months of age.					
33666538	0	92	theme	nutrigenetic	36:47	arg1	effects					49:55	nutrigenetic effects	36:55	nutrigenetic effects	36:55	Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis.					
33666538	9	93	theme	similar	1534:1540	arg1	effects					1553:1559	many similar beneficial effects	1529:1559	many similar beneficial effects	1529:1559	DISCUSSION We found that inulin had many similar beneficial effects in the gut and brain for both E3FAD and E4FAD mice, which may be protective for brain functions and reduce risk for neurodegeneration.					
33666538	8	94	theme	tight	1466:1470	arg1	expression					1481:1490	Claudin-5 BBB tight junction expression	1452:1490	Claudin-5 BBB tight junction expression	1452:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	3	95	theme	APOE4	619:623	arg1	mice					637:640	APOE4 gene (E4FAD mice	619:640	APOE4 gene (E4FAD mice	619:640	METHOD We performed experiments with young mice expressing the human APOE3 (E3FAD mice and APOE4 gene (E4FAD mice).					
33666538	2	96	theme	potential	398:406	arg1	modulation					408:417	the potential modulation	394:417	the potential modulation of the systemic metabolism and neuroprotection	394:464	Specifically, we compared responses to inulin for the potential modulation of the systemic metabolism and neuroprotection via gut-brain axis in mice with human APOE ϵ3 and ϵ4 alleles.					
33666538	1	97	theme	different	286:294	arg1	variants					334:341	different human apolipoprotein E (APOE) genetic variants	286:341	different human apolipoprotein E (APOE) genetic variants	286:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	8	98	theme	expression	1481:1490	arg1	levels					1428:1433	levels	1428:1433	levels of Claudin-1 and Claudin-5 BBB tight junction expression	1428:1490	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	8	98	theme	expression	1481:1490	arg1	levels					1367:1372	levels	1367:1372	levels of brain metabolites related to oxidative stress	1367:1421	Differences were found in levels of brain metabolites related to oxidative stress, and levels of Claudin-1 and Claudin-5 BBB tight junction expression.					
33666538	1	99	theme	apolipoprotein	302:315	arg1	APOE					320:323	APOE	320:323	APOE	320:323	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	1	99	theme	apolipoprotein	302:315	arg1	E					317:317	human apolipoprotein E	296:317	different human apolipoprotein E (APOE) genetic variants	286:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	7	100	theme	related	1200:1206	arg1	metabolism					1189:1198	altered metabolism	1181:1198	altered metabolism related to tryptophan and tyrosine	1181:1233	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	10	101	contain	had	1724:1726	arg1	mice					1714:1717	E3FAD and E4FAD mice	1698:1717	E3FAD and E4FAD mice	1698:1717	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	10	101	contain	had	1724:1726	arg2	responses					1737:1745	distinct responses	1728:1745	distinct responses	1728:1745	E3FAD and E4FAD mice also had distinct responses in several metabolic pathways, suggesting an APOE-dependent nutrigenetic effects in modulating systemic metabolism and neuroprotection.					
33666538	6	102	theme	hippocampal	1137:1147	arg1	scyllo-inositol					1149:1163	hippocampal scyllo-inositol	1137:1163	hippocampal scyllo-inositol	1137:1163	RESULTS In both E3FAD and E4FAD mice, inulin altered the alpha and beta diversity of the gut microbiome, increased beneficial taxa of bacteria and elevated cecal short chain fatty acid and hippocampal scyllo-inositol.					
33666538	7	103	theme	altered	1181:1187	arg1	metabolism					1189:1198	altered metabolism	1181:1198	altered metabolism related to tryptophan and tyrosine	1181:1233	E3FAD mice had altered metabolism related to tryptophan and tyrosine, while E4FAD mice had changes in the tricarboxylic acid cycle, pentose phosphate pathway, and bile acids.					
33666538	1	104	theme	nutrigenetic	222:233	arg1	effects					235:241	the potential nutrigenetic effects	208:241	the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants	208:341	OBJECTIVE The goal of the study was to identify the potential nutrigenetic effects to inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants.					
33666538	5	105	theme	Western	934:940	arg1	blot					942:945	Western blot	934:945	Western blot	934:945	We determined gut microbiome diversity and composition using16s rRNA sequencing, systemic metabolism using in vivo MRI and metabolomics, and blood-brain barrier (BBB) tight junction expression using Western blot.					
33666538	4	106	theme	age	730:732	arg1	months					720:725	3 months	718:725	3 months of age	718:732	We fed mice with either inulin or control diet for 16 weeks starting from 3 months of age.					
32908160	2	0	theme	unstable	586:593	arg1	cytokine					595:602	unstable cytokine	586:602	unstable cytokine	586:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	10	1	theme	smoother	2071:2078	arg1	surface					2080:2086	a smoother surface	2069:2086	a smoother surface	2069:2086	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	14	2	theme	complement	2897:2906	arg1	levels					2887:2892	the levels	2883:2892	the levels of complement and inflammation factors	2883:2931	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	2	3	from	membranes	465:473	arg1	series					414:419	a series	412:419	a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	412:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	8	4	theme	1L-1α	1712:1716	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	1	5	theme	life-sustaining	244:258	arg1	treatment					260:268	a life-sustaining treatment	242:268	a life-sustaining treatment	242:268	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	10	6	theme	broad	2227:2231	arg1	spectrum					2233:2240	a broad spectrum	2225:2240	a broad spectrum of uremic toxins	2225:2257	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	11	7	theme	greater	2293:2299	arg1	hydrophilicity					2301:2314	greater hydrophilicity	2293:2314	greater hydrophilicity	2293:2314	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	8	8	theme	1L-1β	1719:1723	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	12	9	theme	docking	2476:2482	arg1	studies					2484:2490	Molecular docking studies	2466:2490	Molecular docking studies	2466:2490	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	14	10	theme	dialysis	2802:2809	arg1	session					2811:2817	a single dialysis session	2793:2817	a single dialysis session	2793:2817	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	14	11	theme	milder	2943:2948	arg1	extent					2950:2955	a milder extent	2941:2955	a milder extent	2941:2955	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	8	12	theme	TNF-α	1726:1730	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	14	13	theme	biocompatible	2837:2849	arg1	membrane					2851:2858	a more biocompatible membrane	2830:2858	a more biocompatible membrane such as CTA	2830:2870	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	7	14	theme	inflammatory	1486:1497	arg1	biomarkers					1499:1508	inflammatory biomarkers	1486:1508	inflammatory biomarkers released	1486:1517	Samples were collected from dialysis patients to ascertain the extent of inflammatory biomarkers released, before, during (30 and 90 min) and after dialysis (4 h).					
32908160	4	15	theme	HD	866:867	arg1	treatment					869:877	HD treatment	866:877	HD treatment	866:877	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	7	16	theme	dialysis	1441:1448	arg1	patients					1450:1457	dialysis patients	1441:1457	dialysis patients	1441:1457	Samples were collected from dialysis patients to ascertain the extent of inflammatory biomarkers released, before, during (30 and 90 min) and after dialysis (4 h).					
32908160	8	17	theme	IL6	1733:1735	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	10	18	theme	PVP	2127:2129	arg1	membranes					2131:2139	PVP membranes	2127:2139	PVP membranes	2127:2139	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	5	19	theme	innovative	1060:1069	arg1	study					1071:1075	this innovative study	1055:1075	this innovative study	1055:1075	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	13	20	theme	greater	2636:2642	arg1	adsorption					2644:2653	slower but greater adsorption	2625:2653	slower but greater adsorption of fibrinogen	2625:2667	PAES: PVP membranes result in slower but greater adsorption of fibrinogen, but are more likely to experience reversible and irreversible fouling as well as backfiltration.					
32908160	10	21	dep	in	2007:2008	arg1	vitro					2010:2014	vitro	2010:2014	vitro	2010:2014	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	14	22	theme	major	2771:2775	arg1	finding					2777:2783	Our major finding	2767:2783	Our major finding	2767:2783	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	10	23	theme	chemical	1978:1985	arg1	analyses					2027:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	10	24	contain	has	2154:2156	arg2	distribution					2176:2187	smaller pore size distribution	2158:2187	smaller pore size distribution	2158:2187	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	10	24	contain	has	2154:2156	arg1	it					2151:2152	it	2151:2152	it	2151:2152	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	10	24	contain	has	2154:2156	arg1	membranes					2131:2139	PVP membranes	2127:2139	PVP membranes	2127:2139	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	0	25	theme	inflammatory	146:157	arg1	biomarkers					159:168	inflammatory biomarkers	146:168	inflammatory biomarkers	146:168	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	1	26	theme	patient	372:378	arg1	outcomes					380:387	severe patient outcomes	365:387	severe patient outcomes	365:387	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	9	27	theme	uremic	1774:1779	arg1	blood					1781:1785	uremic blood	1774:1785	uremic blood in vitro	1774:1794	We have likewise incubated uremic blood in vitro with the two membrane materials to determine the impact that membrane materials pose in favor of activation away from the hydrodynamics influences.					
32908160	1	28	theme	HD	275:276	arg1	properties					287:296	HD membrane properties	275:296	HD membrane properties	275:296	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	11	29	theme	membrane	2379:2386	arg1	surface					2388:2394	the membrane surface	2375:2394	the membrane surface	2375:2394	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	10	30	theme	morphological	1963:1975	arg1	analyses					2027:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	6	31	theme	different	1326:1334	arg1	membranes					1348:1356	different clinical HD membranes	1326:1356	different clinical HD membranes	1326:1356	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	3	32	theme	composition	791:801	arg1	terms					758:762	terms	758:762	terms of morphology and chemical composition	758:801	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	6	33	theme	fibrinogen	1312:1321	arg1	adsorption					1298:1307	In-vitro adsorption	1289:1307	In-vitro adsorption of fibrinogen on different clinical HD membranes	1289:1356	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	3	34	theme	HD	614:615	arg1	membranes					617:625	Clinical HD membranes	605:625	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP)	605:723	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	2	35	theme	available	475:483	arg1	membranes					465:473	the common clinical HD membranes	442:473	the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	442:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	2	36	from	hospitals	497:505	arg1	available					475:483	available	475:483	available	475:483	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	10	37	theme	pore	2166:2169	arg1	size					2171:2174	smaller pore size	2158:2174	smaller pore size distribution	2158:2187	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	4	38	with	interactions	821:832	arg1	samples					852:858	uremic blood samples	839:858	uremic blood samples after HD treatment	839:877	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	4	39	dep	Infra-Red	915:923	arg1	FTIR					926:929	FTIR	926:929	FTIR	926:929	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	1	40	theme	End	171:173	arg1	ESRD					196:199	ESRD	196:199	ESRD	196:199	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	1	40	theme	End	171:173	arg1	disease					187:193	End stage renal disease	171:193	End stage renal disease (ESRD) patients	171:209	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	2	41	theme	HD	462:463	arg1	membranes					465:473	the common clinical HD membranes	442:473	the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	442:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	5	42	theme	models	1182:1187	arg1	interactions					1132:1143	the interactions	1128:1143	the interactions of human blood proteins and membrane models	1128:1187	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	10	43	theme	analyses	2027:2034	arg1	results					1948:1954	The results	1944:1954	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses	1944:2034	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	0	44	theme	morphology	72:81	arg1	influences					49:58	influences	49:58	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.	0:169	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	1	45	theme	renal	181:185	arg1	ESRD					196:199	ESRD	196:199	ESRD	196:199	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	1	45	theme	renal	181:185	arg1	disease					187:193	End stage renal disease	171:193	End stage renal disease (ESRD) patients	171:209	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	2	46	from	series	414:419	arg1	membranes					465:473	the common clinical HD membranes	442:473	the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	442:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	11	47	theme	membranes	2329:2337	arg1	surface					2281:2287	the rougher surface	2269:2287	the rougher surface	2269:2287	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	11	47	theme	membranes	2329:2337	arg1	hydrophilicity					2301:2314	greater hydrophilicity	2293:2314	greater hydrophilicity	2293:2314	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	0	48	theme	biocompatibility	87:102	arg1	influences					49:58	influences	49:58	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.	0:169	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	2	49	theme	Canadian	488:495	arg1	hospitals					497:505	Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	488:602	Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	488:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	2	50	from	investigations	424:437	arg1	membranes					465:473	the common clinical HD membranes	442:473	the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	442:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	12	51	theme	proteins	2571:2578	arg1	adsorption					2557:2566	the adsorption	2553:2566	the adsorption of proteins and receptors	2553:2592	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	11	52	from	surface	2388:2394	arg1	rupture					2364:2370	rupture	2364:2370	rupture	2364:2370	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	0	53	theme	uremic	107:112	arg1	interactions					129:140	uremic blood-membrane interactions	107:140	uremic blood-membrane interactions	107:140	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	5	54	theme	Modeling	1100:1107	arg1	Docking					1109:1115	Molecular Modeling Docking	1090:1115	Molecular Modeling Docking	1090:1115	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	12	55	theme	important	2535:2543	arg1	role					2545:2548	an important role	2532:2548	an important role	2532:2548	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	0	56	theme	Case	0:3	arg1	studies					5:11	Case studies	0:11	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.	0:169	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	14	57	dep	complement	2897:2906	arg1	factors					2925:2931	factors	2925:2931	factors	2925:2931	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	11	58	theme	red	2349:2351	arg1	cell					2359:2362	red blood cell	2349:2362	red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions	2349:2463	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	12	59	theme	receptors	2584:2592	arg1	adsorption					2557:2566	the adsorption	2553:2566	the adsorption of proteins and receptors	2553:2592	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	1	60	theme	membrane	278:285	arg1	properties					287:296	HD membrane properties	275:296	HD membrane properties	275:296	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	2	61	theme	common	446:451	arg1	membranes					465:473	the common clinical HD membranes	442:473	the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	442:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	3	62	theme	cellulose	639:647	arg1	CTA					661:663	CTA	661:663	CTA	661:663	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	3	62	theme	cellulose	639:647	arg1	triacetate					649:658	cellulose triacetate	639:658	cellulose triacetate (CTA)	639:664	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	0	63	theme	hemodialysis	25:36	arg1	membranes					38:46	clinical hemodialysis membranes	16:46	clinical hemodialysis membranes	16:46	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	10	64	theme	spectroscopic	1988:2000	arg1	analyses					2027:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	8	65	theme	Collected	1577:1585	arg1	samples					1593:1599	Collected blood samples	1577:1599	Collected blood samples	1577:1599	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	4	66	theme	uremic	839:844	arg1	samples					852:858	uremic blood samples	839:858	uremic blood samples after HD treatment	839:877	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	14	67	theme	inflammation	2912:2923	arg1	levels					2887:2892	the levels	2883:2892	the levels of complement and inflammation factors	2883:2931	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	5	68	theme	blood	1154:1158	arg1	proteins					1160:1167	human blood proteins	1148:1167	human blood proteins	1148:1167	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	11	69	theme	rougher	2273:2279	arg1	surface					2281:2287	the rougher surface	2269:2287	the rougher surface	2269:2287	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	13	70	theme	reversible	2704:2713	arg1	fouling					2732:2738	reversible and irreversible fouling	2704:2738	reversible and irreversible fouling as well as backfiltration	2704:2764	PAES: PVP membranes result in slower but greater adsorption of fibrinogen, but are more likely to experience reversible and irreversible fouling as well as backfiltration.					
32908160	14	71	theme	PAES	2978:2981	arg1	membrane					2983:2990	a PAES membrane	2976:2990	a PAES membrane	2976:2990	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	3	72	dep	polyvinylpyrrolidone	670:689	arg1	polyarylethersulfone					692:711	polyarylethersulfone	692:711	polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP)	670:723	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	12	73	theme	Molecular	2466:2474	arg1	studies					2484:2490	Molecular docking studies	2466:2490	Molecular docking studies	2466:2490	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	13	74	theme	irreversible	2719:2730	arg1	fouling					2732:2738	reversible and irreversible fouling	2704:2738	reversible and irreversible fouling as well as backfiltration	2704:2764	PAES: PVP membranes result in slower but greater adsorption of fibrinogen, but are more likely to experience reversible and irreversible fouling as well as backfiltration.					
32908160	5	75	theme	group	1237:1241	arg1	types					1243:1247	functional group types	1226:1247	functional group types responsible for perceived interactions	1226:1286	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	5	76	theme	human	1148:1152	arg1	proteins					1160:1167	human blood proteins	1148:1167	human blood proteins	1148:1167	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	5	77	used	utilized	1081:1088	arg2	we					1078:1079	we	1078:1079	we	1078:1079	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	9	78	theme	membrane	1857:1864	arg1	materials					1866:1874	membrane materials	1857:1874	membrane materials	1857:1874	We have likewise incubated uremic blood in vitro with the two membrane materials to determine the impact that membrane materials pose in favor of activation away from the hydrodynamics influences.					
32908160	8	79	theme	Luminex	1621:1627	arg1	assays					1629:1634	Luminex assays	1621:1634	Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1621:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	11	80	theme	biochemical	2435:2445	arg1	reactions					2455:2463	biochemical cascade reactions	2435:2463	biochemical cascade reactions	2435:2463	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	10	81	theme	spectrum	2233:2240	arg1	clearance					2212:2220	poor clearance	2207:2220	poor clearance of a broad spectrum of uremic toxins	2207:2257	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	12	82	theme	sulfone	2501:2507	arg1	groups					2520:2525	sulfone functional groups	2501:2525	sulfone functional groups	2501:2525	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	6	83	theme	In-vitro	1289:1296	arg1	adsorption					1298:1307	In-vitro adsorption	1289:1307	In-vitro adsorption of fibrinogen on different clinical HD membranes	1289:1356	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	5	84	theme	in-depth	1200:1207	arg1	understanding					1209:1221	an in-depth understanding	1197:1221	an in-depth understanding of functional group types responsible for perceived interactions	1197:1286	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	7	85	theme	biomarkers	1499:1508	arg1	extent					1476:1481	the extent	1472:1481	the extent of inflammatory biomarkers released	1472:1517	Samples were collected from dialysis patients to ascertain the extent of inflammatory biomarkers released, before, during (30 and 90 min) and after dialysis (4 h).					
32908160	2	86	theme	blood	565:569	arg1	activation					571:580	blood activation	565:580	blood activation	565:580	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	8	87	theme	inflammatory	1644:1655	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	13	88	theme	fibrinogen	2658:2667	arg1	adsorption					2644:2653	slower but greater adsorption	2625:2653	slower but greater adsorption of fibrinogen	2625:2667	PAES: PVP membranes result in slower but greater adsorption of fibrinogen, but are more likely to experience reversible and irreversible fouling as well as backfiltration.					
32908160	1	89	theme	disease	187:193	arg1	patients					202:209	End stage renal disease (ESRD) patients	171:209	End stage renal disease (ESRD) patients	171:209	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	2	90	theme	investigations	424:437	arg1	series					414:419	a series	412:419	a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	412:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	4	91	theme	blood	846:850	arg1	samples					852:858	uremic blood samples	839:858	uremic blood samples after HD treatment	839:877	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	14	92	theme	single	2795:2800	arg1	session					2811:2817	a single dialysis session	2793:2817	a single dialysis session	2793:2817	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	10	93	theme	higher	2092:2097	arg1	biocompatibility					2099:2114	higher biocompatibility	2092:2114	higher biocompatibility than PAES	2092:2124	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	1	94	theme	critical	305:312	arg1	role					314:317	a critical role	303:317	a critical role	303:317	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	4	95	dep	Fourier	897:903	arg1	Transform					905:913	Transform	905:913	Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers	905:1028	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	10	96	theme	uremic	2245:2250	arg1	toxins					2252:2257	uremic toxins	2245:2257	uremic toxins	2245:2257	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	6	97	dep	different	1326:1334	arg1	clinical					1336:1343	clinical	1336:1343	clinical	1336:1343	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	2	98	theme	key	522:524	arg1	reasons					526:532	the key reasons	518:532	the key reasons behind their susceptibility to blood activation and unstable cytokine	518:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	9	99	theme	hydrodynamics	1918:1930	arg1	influences					1932:1941	the hydrodynamics influences	1914:1941	the hydrodynamics influences	1914:1941	We have likewise incubated uremic blood in vitro with the two membrane materials to determine the impact that membrane materials pose in favor of activation away from the hydrodynamics influences.					
32908160	13	100	theme	slower	2625:2630	arg1	adsorption					2644:2653	slower but greater adsorption	2625:2653	slower but greater adsorption of fibrinogen	2625:2667	PAES: PVP membranes result in slower but greater adsorption of fibrinogen, but are more likely to experience reversible and irreversible fouling as well as backfiltration.					
32908160	1	101	theme	severe	365:370	arg1	outcomes					380:387	severe patient outcomes	365:387	severe patient outcomes	365:387	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	10	102	theme	toxins	2252:2257	arg1	spectrum					2233:2240	a broad spectrum	2225:2240	a broad spectrum of uremic toxins	2225:2257	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	2	103	from	available	475:483	arg1	hospitals					497:505	Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	488:602	Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	488:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	3	104	theme	morphology	767:776	arg1	terms					758:762	terms	758:762	terms of morphology and chemical composition	758:801	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	8	105	theme	vWF	1742:1744	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	11	106	theme	rupture	2364:2370	arg1	cell					2359:2362	red blood cell	2349:2362	red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions	2349:2463	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	3	107	theme	Clinical	605:612	arg1	membranes					617:625	Clinical HD membranes	605:625	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP)	605:723	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	4	108	theme	Infra-Red	915:923	arg1	techniques					956:965	Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers	915:1028	Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers	915:1028	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	10	109	theme	size	2171:2174	arg1	distribution					2176:2187	smaller pore size distribution	2158:2187	smaller pore size distribution	2158:2187	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	0	110	theme	membrane	63:70	arg1	morphology					72:81	membrane morphology	63:81	membrane morphology	63:81	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	12	111	theme	functional	2509:2518	arg1	groups					2520:2525	sulfone functional groups	2501:2525	sulfone functional groups	2501:2525	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	10	112	theme	smaller	2158:2164	arg1	size					2171:2174	smaller pore size	2158:2174	smaller pore size distribution	2158:2187	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	5	113	theme	responsible	1249:1259	arg1	types					1243:1247	functional group types	1226:1247	functional group types responsible for perceived interactions	1226:1286	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	6	114	theme	clinical	1382:1389	arg1	conditions					1401:1410	similar clinical operating conditions	1374:1410	similar clinical operating conditions	1374:1410	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	1	115	theme	blood	322:326	arg1	activation					328:337	blood activation	322:337	blood activation	322:337	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	1	116	theme	stage	175:179	arg1	ESRD					196:199	ESRD	196:199	ESRD	196:199	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	1	116	theme	stage	175:179	arg1	disease					187:193	End stage renal disease	171:193	End stage renal disease (ESRD) patients	171:209	End stage renal disease (ESRD) patients depend on hemodialysis (HD) as a life-sustaining treatment, but HD membrane properties play a critical role in blood activation during HD and can lead to severe patient outcomes.					
32908160	2	117	theme	clinical	453:460	arg1	membranes					465:473	the common clinical HD membranes	442:473	the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine	442:602	This study reports on a series of investigations on the common clinical HD membranes available in Canadian hospitals to explore the key reasons behind their susceptibility to blood activation and unstable cytokine.					
32908160	11	118	theme	protein	2412:2418	arg1	adsorption					2420:2429	protein adsorption	2412:2429	protein adsorption	2412:2429	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	11	119	theme	PVP	2325:2327	arg1	membranes					2329:2337	PAES: PVP membranes	2319:2337	PAES: PVP membranes	2319:2337	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	5	120	theme	perceived	1265:1273	arg1	interactions					1275:1286	perceived interactions	1265:1286	perceived interactions	1265:1286	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	10	121	theme	incubation	2016:2025	arg1	analyses					2027:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	6	122	theme	operating	1391:1399	arg1	conditions					1401:1410	similar clinical operating conditions	1374:1410	similar clinical operating conditions	1374:1410	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	12	123	dep	indicate	2492:2499	arg1	play					2527:2530	play	2527:2530	indicate sulfone functional groups play an important role in the adsorption of proteins and receptors	2492:2592	Molecular docking studies indicate sulfone functional groups play an important role in the adsorption of proteins and receptors.					
32908160	4	124	theme	spectroscopic	942:954	arg1	techniques					956:965	Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers	915:1028	Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers	915:1028	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	0	125	from	influences	49:58	arg1	interactions					129:140	uremic blood-membrane interactions	107:140	uremic blood-membrane interactions	107:140	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	0	125	from	influences	49:58	arg1	biomarkers					159:168	inflammatory biomarkers	146:168	inflammatory biomarkers	146:168	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	11	126	theme	PAES	2319:2322	arg1	membranes					2329:2337	PAES: PVP membranes	2319:2337	PAES: PVP membranes	2319:2337	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	10	127	theme	in	2007:2008	arg1	analyses					2027:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	our morphological, chemical, spectroscopic, and in vitro incubation analyses	1959:2034	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	6	128	theme	similar	1374:1380	arg1	conditions					1401:1410	similar clinical operating conditions	1374:1410	similar clinical operating conditions	1374:1410	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	5	129	theme	Molecular	1090:1098	arg1	Docking					1109:1115	Molecular Modeling Docking	1090:1115	Molecular Modeling Docking	1090:1115	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	11	130	theme	cascade	2447:2453	arg1	reactions					2455:2463	biochemical cascade reactions	2435:2463	biochemical cascade reactions	2435:2463	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	11	131	theme	blood	2353:2357	arg1	cell					2359:2362	red blood cell	2349:2362	red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions	2349:2463	However, the rougher surface and greater hydrophilicity of PAES: PVP membranes increases red blood cell rupture at the membrane surface, which promotes protein adsorption and biochemical cascade reactions.					
32908160	4	132	from	changes	990:996	arg1	chemistry					1001:1009	chemistry	1001:1009	chemistry	1001:1009	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	4	132	from	changes	990:996	arg1	fibers					1023:1028	membrane fibers	1014:1028	membrane fibers	1014:1028	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	0	133	theme	blood-membrane	114:127	arg1	interactions					129:140	uremic blood-membrane interactions	107:140	uremic blood-membrane interactions	107:140	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	10	134	theme	poor	2207:2210	arg1	clearance					2212:2220	poor clearance	2207:2220	poor clearance of a broad spectrum of uremic toxins	2207:2257	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	5	135	theme	study	1071:1075	arg1	part					1047:1050	part	1047:1050	part of this innovative study	1047:1075	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	0	136	theme	clinical	16:23	arg1	membranes					38:46	clinical hemodialysis membranes	16:46	clinical hemodialysis membranes	16:46	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	5	137	theme	membrane	1173:1180	arg1	models					1182:1187	membrane models	1173:1187	membrane models	1173:1187	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	0	138	theme	membranes	38:46	arg1	studies					5:11	Case studies	0:11	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.	0:169	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	9	139	theme	membrane	1809:1816	arg1	materials					1818:1826	the two membrane materials	1801:1826	the two membrane materials	1801:1826	We have likewise incubated uremic blood in vitro with the two membrane materials to determine the impact that membrane materials pose in favor of activation away from the hydrodynamics influences.					
32908160	8	140	theme	blood	1587:1591	arg1	samples					1593:1599	Collected blood samples	1577:1599	Collected blood samples	1577:1599	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	4	141	theme	Membrane-surface	804:819	arg1	interactions					821:832	Membrane-surface interactions	804:832	Membrane-surface interactions with uremic blood samples after HD treatment	804:877	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	3	142	theme	chemical	782:789	arg1	composition					791:801	chemical composition	782:801	chemical composition	782:801	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	3	143	dep	PAES	714:717	arg1	PVP					720:722	PVP	720:722	PVP	720:722	Clinical HD membranes composed of cellulose triacetate (CTA) and polyvinylpyrrolidone: polyarylethersulfone (PAES: PVP) were thoroughly characterized in terms of morphology and chemical composition.					
32908160	4	144	theme	membrane	1014:1021	arg1	fibers					1023:1028	membrane fibers	1014:1028	membrane fibers	1014:1028	Membrane-surface interactions with uremic blood samples after HD treatment were probed using Fourier Transform Infra-Red (FTIR) and Raman spectroscopic techniques in order to understand changes in chemistry on membrane fibers.					
32908160	13	145	theme	PVP	2601:2603	arg1	membranes					2605:2613	PVP membranes	2601:2613	PVP membranes	2601:2613	PAES: PVP membranes result in slower but greater adsorption of fibrinogen, but are more likely to experience reversible and irreversible fouling as well as backfiltration.					
32908160	10	146	contain	have	2064:2067	arg2	surface					2080:2086	a smoother surface	2069:2086	a smoother surface	2069:2086	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	10	146	contain	have	2064:2067	arg1	membranes					2054:2062	CTA membranes	2050:2062	CTA membranes	2050:2062	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	10	146	contain	have	2064:2067	arg2	biocompatibility					2099:2114	higher biocompatibility	2092:2114	higher biocompatibility than PAES	2092:2124	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	8	147	theme	Serpin/Antithrombin-III	1671:1693	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	5	148	theme	proteins	1160:1167	arg1	interactions					1132:1143	the interactions	1128:1143	the interactions of human blood proteins and membrane models	1128:1187	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	10	149	theme	CTA	2050:2052	arg1	membranes					2054:2062	CTA membranes	2050:2062	CTA membranes	2050:2062	The results of our morphological, chemical, spectroscopic, and in vitro incubation analyses indicate that CTA membranes have a smoother surface and higher biocompatibility than PAES: PVP membranes, however, it has smaller pore size distribution, which results in poor clearance of a broad spectrum of uremic toxins.					
32908160	6	150	theme	HD	1345:1346	arg1	membranes					1348:1356	different clinical HD membranes	1326:1356	different clinical HD membranes	1326:1356	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	8	151	theme	Properdin	1696:1704	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	5	152	theme	functional	1226:1235	arg1	types					1243:1247	functional group types	1226:1247	functional group types responsible for perceived interactions	1226:1286	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
32908160	0	153	dep	studies	5:11	arg1	influences					49:58	influences	49:58	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.	0:169	Case studies of clinical hemodialysis membranes: influences of membrane morphology and biocompatibility on uremic blood-membrane interactions and inflammatory biomarkers.					
32908160	14	154	with	dialysis	2962:2969	arg1	membrane					2983:2990	a PAES membrane	2976:2990	a PAES membrane	2976:2990	Our major finding is that a single dialysis session, even with a more biocompatible membrane such as CTA, increases the levels of complement and inflammation factors, but to a milder extent than dialysis with a PAES membrane.					
32908160	6	155	from	adsorption	1298:1307	arg1	membranes					1348:1356	different clinical HD membranes	1326:1356	different clinical HD membranes	1326:1356	In-vitro adsorption of fibrinogen on different clinical HD membranes was compared at similar clinical operating conditions.					
32908160	8	156	theme	C5a	1707:1709	arg1	biomarkers					1657:1666	the inflammatory biomarkers	1640:1666	the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF	1640:1744	Collected blood samples were analyzed using Luminex assays for the inflammatory biomarkers of Serpin/Antithrombin-III, Properdin, C5a, 1L-1α, 1L-1β, TNF-α, IL6, and vWF.					
32908160	5	157	theme	types	1243:1247	arg1	understanding					1209:1221	an in-depth understanding	1197:1221	an in-depth understanding of functional group types responsible for perceived interactions	1197:1286	In addition, as part of this innovative study, we utilized Molecular Modeling Docking to examine the interactions of human blood proteins and membrane models to gain an in-depth understanding of functional group types responsible for perceived interactions.					
34694067	7	0	theme	endothelial	1113:1123	arg1	factor					1132:1137	vascular endothelial growth factor	1104:1137	vascular endothelial growth factor secretion	1104:1147	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	2	1	theme	Implanting	336:345	arg1	biomaterials					347:358	Implanting biomaterials	336:358	Implanting biomaterials	336:358	Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON.					
34694067	9	2	theme	MiR	1419:1421	arg1	TDNs/Li-hep-gel					1423:1437	MiR@TDNs/Li-hep-gel	1419:1437	MiR@TDNs/Li-hep-gel	1419:1437	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	6	3	theme	stem	888:891	arg1	BMSCs					900:904	BMSCs	900:904	BMSCs	900:904	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	6	3	theme	stem	888:891	arg1	cells					893:897	bone mesenchymal stem cells	871:897	bone mesenchymal stem cells (BMSCs)	871:905	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	4	4	theme	delivery	683:690	arg1	hydrogel					613:620	Heparin lithium hydrogel	597:620	Heparin lithium hydrogel (Li-hep-gel)	597:633	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	4	theme	delivery	683:690	arg1	agent					692:696	a lithium and MiR@TDNs delivery agent	660:696	a lithium and MiR@TDNs delivery agent	660:696	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	3	5	theme	DKK1	579:582	arg1	translation					584:594	DKK1 translation	579:594	DKK1 translation	579:594	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	6	6	theme	bone	871:874	arg1	BMSCs					900:904	BMSCs	900:904	BMSCs	900:904	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	6	6	theme	bone	871:874	arg1	cells					893:897	bone mesenchymal stem cells	871:897	bone mesenchymal stem cells (BMSCs)	871:905	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	1	7	theme	Wnt	231:233	arg1	inhibition					245:254	Wnt signaling inhibition	231:254	Wnt signaling inhibition	231:254	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	8	8	theme	MiR	1203:1205	arg1	TDNs/Li-hep-gel					1207:1221	MiR@TDNs/Li-hep-gel	1203:1221	MiR@TDNs/Li-hep-gel	1203:1221	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	7	9	theme	droplet	1063:1069	arg1	expression					1071:1080	lipid droplet expression	1057:1080	lipid droplet expression of BMSCs	1057:1089	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	2	10	theme	bone	432:435	arg1	defects					437:443	repair challenging bone defects	413:443	repair challenging bone defects associated with SAON	413:464	Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON.					
34694067	8	11	theme	bone	1270:1273	arg1	defect					1275:1280	the internal bone defect	1257:1280	the internal bone defect of osteonecrosis	1257:1297	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	5	12	theme	bone	742:745	arg1	defect					747:752	challenging bone defect	730:752	challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite	730:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	5	13	from	defect	747:752	arg1	SAON					757:760	SAON	757:760	SAON using a MiR@TDNs/Li-hep-gel composite	757:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	7	14	theme	vascular-like	1153:1165	arg1	formation					1177:1185	vascular-like structure formation	1153:1185	vascular-like structure formation in vitro	1153:1194	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	7	15	theme	phosphatase	993:1003	arg1	expression					1005:1014	alkaline phosphatase expression	984:1014	alkaline phosphatase expression	984:1014	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	9	16	theme	lithium	1468:1474	arg1	delivery					1456:1463	dual delivery	1451:1463	dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair	1451:1648	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	9	17	theme	MiR	1480:1482	arg1	TDNs					1484:1487	MiR@TDNs	1480:1487	MiR@TDNs	1480:1487	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	7	18	theme	calcium	1020:1026	arg1	deposition					1036:1045	calcium nodular deposition	1020:1045	calcium nodular deposition	1020:1045	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	7	19	theme	@	970:970	arg1	TDNs					971:974	MiR@TDNs	967:974	MiR@TDNs	967:974	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	9	20	theme	TDNs	1484:1487	arg1	delivery					1456:1463	dual delivery	1451:1463	dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair	1451:1648	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	5	21	theme	MiR	770:772	arg1	composite					790:798	a MiR@TDNs/Li-hep-gel composite	768:798	a MiR@TDNs/Li-hep-gel composite	768:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	7	22	theme	structure	1167:1175	arg1	formation					1177:1185	vascular-like structure formation	1153:1185	vascular-like structure formation in vitro	1153:1194	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	1	23	theme	Dickkopf-1	196:205	arg1	upregulation					214:225	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation	172:225	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation	172:225	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	5	24	theme	repair	712:717	arg1	effects					719:725	the repair effects	708:725	the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite	708:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	8	25	theme	large	1318:1322	arg1	area					1324:1327	a large area	1316:1327	a large area of new bone accompanied with neovascularization and reduced empty lacunae	1316:1401	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	0	26	theme	Lithium	89:95	arg1	Hydrogel					97:104	an Injectable Heparin Lithium Hydrogel	67:104	an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis	67:169	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	9	27	theme	Wnt	1527:1529	arg1	pathway					1541:1547	the Wnt signaling pathway	1523:1547	the Wnt signaling pathway	1523:1547	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	8	28	theme	bone	1336:1339	arg1	area					1324:1327	a large area	1316:1327	a large area of new bone accompanied with neovascularization and reduced empty lacunae	1316:1401	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	0	29	theme	Bone	122:125	arg1	Defects					127:133	Bone Defects	122:133	Bone Defects in Steroid-Associated Osteonecrosis	122:169	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	3	30	theme	tetrahedral	473:483	arg1	nanostructures					489:502	tetrahedral DNA nanostructures	473:502	tetrahedral DNA nanostructures (TDNs)	473:509	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	3	30	theme	tetrahedral	473:483	arg1	carriers					534:541	gene carriers	529:541	gene carriers	529:541	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	3	30	theme	tetrahedral	473:483	arg1	TDNs					505:508	TDNs	505:508	TDNs	505:508	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	4	31	theme	Heparin	597:603	arg1	agent					692:696	a lithium and MiR@TDNs delivery agent	660:696	a lithium and MiR@TDNs delivery agent	660:696	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	31	theme	Heparin	597:603	arg1	Li-hep-gel					623:632	Li-hep-gel	623:632	Li-hep-gel	623:632	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	31	theme	Heparin	597:603	arg1	hydrogel					613:620	Heparin lithium hydrogel	597:620	Heparin lithium hydrogel (Li-hep-gel)	597:633	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	0	32	theme	MiR335-5p-Pendant	12:28	arg1	Nanostructures					46:59	MiR335-5p-Pendant Tetrahedron DNA Nanostructures	12:59	MiR335-5p-Pendant Tetrahedron DNA Nanostructures	12:59	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	8	33	theme	empty	1389:1393	arg1	lacunae					1395:1401	reduced empty lacunae	1381:1401	reduced empty lacunae	1381:1401	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	0	34	theme	DNA	42:44	arg1	Nanostructures					46:59	MiR335-5p-Pendant Tetrahedron DNA Nanostructures	12:59	MiR335-5p-Pendant Tetrahedron DNA Nanostructures	12:59	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	0	35	theme	Injectable	70:79	arg1	Hydrogel					97:104	an Injectable Heparin Lithium Hydrogel	67:104	an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis	67:169	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	9	36	used	used	1630:1633	arg2	lithium					1468:1474	lithium	1468:1474	lithium	1468:1474	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	2	37	theme	promising	393:401	arg1	method					403:408	a promising method	391:408	a promising method to repair challenging bone defects associated with SAON	391:464	Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON.					
34694067	7	38	theme	factor	1132:1137	arg1	secretion					1139:1147	vascular endothelial growth factor secretion	1104:1147	vascular endothelial growth factor secretion	1104:1147	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	6	39	theme	@	849:849	arg1	TDNs					850:853	MiR@TDNs	846:853	MiR@TDNs	846:853	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	1	40	theme	bone	266:269	arg1	disorder					282:289	bone metabolism disorder	266:289	bone metabolism disorder	266:289	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	2	41	theme	challenging	420:430	arg1	defects					437:443	repair challenging bone defects	413:443	repair challenging bone defects associated with SAON	413:464	Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON.					
34694067	7	42	theme	vascular	1104:1111	arg1	factor					1132:1137	vascular endothelial growth factor	1104:1137	vascular endothelial growth factor secretion	1104:1147	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	1	43	theme	steroid-associated	295:312	arg1	osteonecrosis					314:326	steroid-associated osteonecrosis	295:326	steroid-associated osteonecrosis (SAON)	295:333	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	1	43	theme	steroid-associated	295:312	arg1	SAON					329:332	SAON	329:332	SAON	329:332	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	5	44	from	effects	719:725	arg1	defect					747:752	challenging bone defect	730:752	challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite	730:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	9	45	theme	SAON	1638:1641	arg1	repair					1643:1648	SAON repair	1638:1648	SAON repair	1638:1648	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	7	46	theme	BMSCs	1085:1089	arg1	expression					1071:1080	lipid droplet expression	1057:1080	lipid droplet expression of BMSCs	1057:1089	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	9	47	theme	@	1422:1422	arg1	TDNs/Li-hep-gel					1423:1437	MiR@TDNs/Li-hep-gel	1419:1437	MiR@TDNs/Li-hep-gel	1419:1437	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	6	48	theme	mesenchymal	876:886	arg1	BMSCs					900:904	BMSCs	900:904	BMSCs	900:904	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	6	48	theme	mesenchymal	876:886	arg1	cells					893:897	bone mesenchymal stem cells	871:897	bone mesenchymal stem cells (BMSCs)	871:905	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	4	49	theme	TDNs	678:681	arg1	hydrogel					613:620	Heparin lithium hydrogel	597:620	Heparin lithium hydrogel (Li-hep-gel)	597:633	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	49	theme	TDNs	678:681	arg1	agent					692:696	a lithium and MiR@TDNs delivery agent	660:696	a lithium and MiR@TDNs delivery agent	660:696	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	5	50	theme	TDNs/Li-hep-gel	774:788	arg1	composite					790:798	a MiR@TDNs/Li-hep-gel composite	768:798	a MiR@TDNs/Li-hep-gel composite	768:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	2	51	theme	Wnt	376:378	arg1	pathway					380:386	the Wnt pathway	372:386	the Wnt pathway	372:386	Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON.					
34694067	8	52	theme	SAON	1245:1248	arg1	model					1250:1254	the SAON model	1241:1254	the SAON model	1241:1254	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	0	53	theme	Steroid-Associated	138:155	arg1	Osteonecrosis					157:169	Steroid-Associated Osteonecrosis	138:169	Steroid-Associated Osteonecrosis	138:169	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	1	54	theme	signaling	235:243	arg1	inhibition					245:254	Wnt signaling inhibition	231:254	Wnt signaling inhibition	231:254	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	4	55	theme	lithium	662:668	arg1	hydrogel					613:620	Heparin lithium hydrogel	597:620	Heparin lithium hydrogel (Li-hep-gel)	597:633	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	55	theme	lithium	662:668	arg1	agent					692:696	a lithium and MiR@TDNs delivery agent	660:696	a lithium and MiR@TDNs delivery agent	660:696	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	5	56	theme	challenging	730:740	arg1	defect					747:752	challenging bone defect	730:752	challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite	730:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	8	57	theme	@	1206:1206	arg1	TDNs/Li-hep-gel					1207:1221	MiR@TDNs/Li-hep-gel	1203:1221	MiR@TDNs/Li-hep-gel	1203:1221	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	4	58	theme	MiR	674:676	arg1	TDNs					678:681	MiR@TDNs	674:681	MiR@TDNs	674:681	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	7	59	theme	nodular	1028:1034	arg1	deposition					1036:1045	calcium nodular deposition	1020:1045	calcium nodular deposition	1020:1045	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	6	60	theme	cell	920:923	arg1	viability					925:933	cell viability	920:933	cell viability	920:933	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	7	61	theme	lipid	1057:1061	arg1	expression					1071:1080	lipid droplet expression	1057:1080	lipid droplet expression of BMSCs	1057:1089	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	0	62	from	Defects	127:133	arg1	Osteonecrosis					157:169	Steroid-Associated Osteonecrosis	138:169	Steroid-Associated Osteonecrosis	138:169	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	7	63	theme	alkaline	984:991	arg1	phosphatase					993:1003	alkaline phosphatase	984:1003	alkaline phosphatase expression	984:1014	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	9	64	theme	@	1483:1483	arg1	TDNs					1484:1487	MiR@TDNs	1480:1487	MiR@TDNs	1480:1487	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	8	65	theme	osteonecrosis	1285:1297	arg1	defect					1275:1280	the internal bone defect	1257:1280	the internal bone defect of osteonecrosis	1257:1297	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	0	66	theme	Heparin	81:87	arg1	Hydrogel					97:104	an Injectable Heparin Lithium Hydrogel	67:104	an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis	67:169	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	9	67	theme	dual	1451:1454	arg1	delivery					1456:1463	dual delivery	1451:1463	dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair	1451:1648	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	1	68	theme	Corticosteroids-induced	172:194	arg1	upregulation					214:225	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation	172:225	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation	172:225	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	5	69	theme	@	773:773	arg1	composite					790:798	a MiR@TDNs/Li-hep-gel composite	768:798	a MiR@TDNs/Li-hep-gel composite	768:798	Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo.					
34694067	7	70	theme	MiR	967:969	arg1	TDNs					971:974	MiR@TDNs	967:974	MiR@TDNs	967:974	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	9	71	theme	signaling	1531:1539	arg1	pathway					1541:1547	the Wnt signaling pathway	1523:1547	the Wnt signaling pathway	1523:1547	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	8	72	theme	new	1332:1334	arg1	bone					1336:1339	new bone	1332:1339	new bone accompanied with neovascularization and reduced empty lacunae	1332:1401	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	4	73	theme	lithium	605:611	arg1	agent					692:696	a lithium and MiR@TDNs delivery agent	660:696	a lithium and MiR@TDNs delivery agent	660:696	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	73	theme	lithium	605:611	arg1	Li-hep-gel					623:632	Li-hep-gel	623:632	Li-hep-gel	623:632	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	4	73	theme	lithium	605:611	arg1	hydrogel					613:620	Heparin lithium hydrogel	597:620	Heparin lithium hydrogel (Li-hep-gel)	597:633	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	3	74	theme	DNA	485:487	arg1	nanostructures					489:502	tetrahedral DNA nanostructures	473:502	tetrahedral DNA nanostructures (TDNs)	473:509	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	3	74	theme	DNA	485:487	arg1	carriers					534:541	gene carriers	529:541	gene carriers	529:541	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	3	74	theme	DNA	485:487	arg1	TDNs					505:508	TDNs	505:508	TDNs	505:508	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	0	75	theme	Tetrahedron	30:40	arg1	Nanostructures					46:59	MiR335-5p-Pendant Tetrahedron DNA Nanostructures	12:59	MiR335-5p-Pendant Tetrahedron DNA Nanostructures	12:59	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	9	76	theme	bone	1560:1563	arg1	regeneration					1565:1576	bone regeneration	1560:1576	bone regeneration	1560:1576	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	0	77	theme	Nanostructures	46:59	arg1	Delivery					0:7	Delivery	0:7	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.	0:170	Delivery of MiR335-5p-Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid-Associated Osteonecrosis.					
34694067	4	78	theme	@	677:677	arg1	TDNs					678:681	MiR@TDNs	674:681	MiR@TDNs	674:681	Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent.					
34694067	8	79	theme	reduced	1381:1387	arg1	lacunae					1395:1401	reduced empty lacunae	1381:1401	reduced empty lacunae	1381:1401	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	7	80	theme	growth	1125:1130	arg1	factor					1132:1137	vascular endothelial growth factor	1104:1137	vascular endothelial growth factor secretion	1104:1147	Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro.					
34694067	9	81	theme	bone	1593:1596	arg1	defects					1598:1604	bone defects	1593:1604	bone defects	1593:1604	In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.					
34694067	6	82	theme	MiR	846:848	arg1	TDNs					850:853	MiR@TDNs	846:853	MiR@TDNs	846:853	The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis.					
34694067	3	83	theme	gene	529:532	arg1	carriers					534:541	gene carriers	529:541	gene carriers	529:541	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	3	83	theme	gene	529:532	arg1	nanostructures					489:502	tetrahedral DNA nanostructures	473:502	tetrahedral DNA nanostructures (TDNs)	473:509	Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation.					
34694067	1	84	theme	metabolism	271:280	arg1	disorder					282:289	bone metabolism disorder	266:289	bone metabolism disorder	266:289	Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON).					
34694067	8	85	theme	internal	1261:1268	arg1	defect					1275:1280	the internal bone defect	1257:1280	the internal bone defect of osteonecrosis	1257:1297	After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae.					
34694067	2	86	theme	repair	413:418	arg1	defects					437:443	repair challenging bone defects	413:443	repair challenging bone defects associated with SAON	413:464	Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON.					
32764206	5	0	theme	ribosomal	725:733	arg1	unit					767:770	a 16S ribosomal DNA-based operational taxonomic unit	719:770	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	11	1	theme	GNB	1470:1472	arg1	lower					1512:1516	lower	1512:1516	lower	1512:1516	By contrast, in the subjects whose GF acidity was strong, the GNB abundance in the GF was significantly lower than that in the saliva.					
32764206	11	1	theme	GNB	1470:1472	arg1	abundance					1474:1482	the GNB abundance	1466:1482	the GNB abundance in the GF	1466:1492	By contrast, in the subjects whose GF acidity was strong, the GNB abundance in the GF was significantly lower than that in the saliva.					
32764206	11	2	theme	GF	1443:1444	arg1	acidity					1446:1452	acidity	1446:1452	acidity	1446:1452	By contrast, in the subjects whose GF acidity was strong, the GNB abundance in the GF was significantly lower than that in the saliva.					
32764206	10	3	from	subjects	1301:1308	arg1	abundance					1345:1353	the GNB abundance	1337:1353	the GNB abundance in the GF	1337:1363	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	10	3	from	subjects	1301:1308	arg1	same					1380:1383	same	1380:1383	same	1380:1383	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	2	4	from	composition	287:297	arg1	fluid					321:325	gastric fluid	313:325	the gastric fluid (GF)	309:330	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	2	4	from	composition	287:297	arg1	GF					328:329	GF	328:329	GF	328:329	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	5	5	theme	DNA-based	735:743	arg1	unit					767:770	a 16S ribosomal DNA-based operational taxonomic unit	719:770	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	12	6	theme	LPS	1636:1638	arg1	activity					1640:1647	the prominent LPS activity	1622:1647	the prominent LPS activity in a stomach with weak acidity	1622:1678	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	7	7	dep	RESULTS	865:871	arg1	found					876:880	found	876:880	found	876:880	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	1	8	theme	Gram-negative	163:175	arg1	GNB					187:189	GNB	187:189	GNB	187:189	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB) are highly toxic and induce inflammation.					
32764206	1	8	theme	Gram-negative	163:175	arg1	bacteria					177:184	Gram-negative bacteria	163:184	Gram-negative bacteria (GNB)	163:190	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB) are highly toxic and induce inflammation.					
32764206	5	9	from	composition	691:701	arg1	samples					710:716	the samples	706:716	the samples	706:716	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	8	10	theme	close	1076:1080	arg1	correlation					1082:1092	a close correlation	1074:1092	a close correlation between pH and LPS	1074:1111	Spearman test also demonstrated a close correlation between pH and LPS in those samples (r = 0.872).					
32764206	0	11	from	Increase	0:7	arg1	Stomach					94:100	the Stomach	90:100	the Stomach With Low Acidity	90:117	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	0	11	from	Increase	0:7	arg1	Activity					35:42	the Lipopolysaccharide Activity	12:42	the Lipopolysaccharide Activity	12:42	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	10	12	theme	GF	1316:1317	arg1	acidity					1319:1325	acidity	1319:1325	acidity	1319:1325	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	4	13	theme	LPS	567:569	arg1	activity					571:578	The LPS activity	563:578	The LPS activity	563:578	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	12	14	theme	oral	1592:1595	arg1	cavity					1597:1602	the oral cavity	1588:1602	the oral cavity	1588:1602	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	8	15	theme	Spearman	1042:1049	arg1	test					1051:1054	Spearman test	1042:1054	Spearman test	1042:1054	Spearman test also demonstrated a close correlation between pH and LPS in those samples (r = 0.872).					
32764206	0	16	from	Accumulation	48:59	arg1	Stomach					94:100	the Stomach	90:100	the Stomach With Low Acidity	90:117	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	0	16	from	Accumulation	48:59	arg1	Activity					35:42	the Lipopolysaccharide Activity	12:42	the Lipopolysaccharide Activity	12:42	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	10	17	theme	GNB	1341:1343	arg1	abundance					1345:1353	the GNB abundance	1337:1353	the GNB abundance in the GF	1337:1363	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	10	17	theme	GNB	1341:1343	arg1	same					1380:1383	same	1380:1383	same	1380:1383	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	7	18	theme	GF	909:910	arg1	samples					912:918	the GF samples	905:918	the GF samples	905:918	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	2	19	theme	LPS	270:272	arg1	activity					274:281	the LPS activity	266:281	the LPS activity	266:281	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	4	20	theme	Limulus	641:647	arg1	lysate					659:664	Limulus amebocyte lysate	641:664	Limulus amebocyte lysate	641:664	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	1	21	theme	bacteria	177:184	arg1	Lipopolysaccharides					133:151	INTRODUCTION Lipopolysaccharides	120:151	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB)	120:190	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB) are highly toxic and induce inflammation.					
32764206	1	21	theme	bacteria	177:184	arg1	LPSs					154:157	LPSs	154:157	LPSs	154:157	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB) are highly toxic and induce inflammation.					
32764206	0	22	theme	Low	107:109	arg1	Acidity					111:117	Low Acidity	107:117	Low Acidity	107:117	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	6	23	theme	whole	847:851	arg1	microbiota					853:862	the whole microbiota	843:862	the whole microbiota	843:862	We focused on the genera representing >0.1% of the whole microbiota.					
32764206	7	24	theme	strong	1003:1008	arg1	acidity					1010:1016	strong acidity	1003:1016	strong acidity (approximately < pH 2)	1003:1039	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	7	24	theme	strong	1003:1008	arg1	pH					1035:1036	approximately < pH 2	1019:1038	approximately < pH 2	1019:1038	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	10	25	from	abundance	1345:1353	arg1	GF					1362:1363	the GF	1358:1363	the GF	1358:1363	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	4	26	theme	Factor	629:634	arg1	C					636:636	recombinant Factor C	617:636	recombinant Factor C	617:636	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	11	27	from	abundance	1474:1482	arg1	GF					1491:1492	the GF	1487:1492	the GF	1487:1492	By contrast, in the subjects whose GF acidity was strong, the GNB abundance in the GF was significantly lower than that in the saliva.					
32764206	9	28	from	abundance	1156:1164	arg1	saliva					1180:1185	the saliva	1176:1185	the saliva	1176:1185	The relative abundance of GNB in the saliva showed no significant difference between the subject groups with weak- and strong-acidity GF.					
32764206	6	29	theme	microbiota	853:862	arg1	microbiota					853:862	the whole microbiota	843:862	the whole microbiota	843:862	We focused on the genera representing >0.1% of the whole microbiota.					
32764206	6	29	theme	microbiota	853:862	arg1	%					838:838	>0.1%	834:838	>0.1% of the whole microbiota	834:862	We focused on the genera representing >0.1% of the whole microbiota.					
32764206	2	30	theme	potential	346:354	arg1	toxicity					356:363	the potential toxicity	342:363	the potential toxicity of them accumulated in the stomach	342:398	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	8	31	dep	samples	1122:1128	arg1	r					1131:1131	r	1131:1131	r = 0.872	1131:1139	Spearman test also demonstrated a close correlation between pH and LPS in those samples (r = 0.872).					
32764206	7	32	theme	high	884:887	arg1	activity					893:900	a high LPS activity	882:900	a high LPS activity in the GF samples	882:918	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	3	33	theme	nasogastric	545:555	arg1	tube					557:560	a nasogastric tube	543:560	a nasogastric tube	543:560	METHODS GF and saliva samples were obtained from 158 outpatients who were undergoing upper gastrointestinal endoscopy and 36 volunteers using a nasogastric tube.					
32764206	0	34	theme	Lipopolysaccharide	16:33	arg1	Activity					35:42	the Lipopolysaccharide Activity	12:42	the Lipopolysaccharide Activity	12:42	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	0	35	with	Stomach	94:100	arg1	Acidity					111:117	Low Acidity	107:117	Low Acidity	107:117	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	2	36	theme	GNB	302:304	arg1	activity					274:281	the LPS activity	266:281	the LPS activity	266:281	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	2	36	theme	GNB	302:304	arg1	composition					287:297	composition	287:297	composition	287:297	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	9	37	theme	subject	1232:1238	arg1	groups					1240:1245	the subject groups	1228:1245	the subject groups	1228:1245	The relative abundance of GNB in the saliva showed no significant difference between the subject groups with weak- and strong-acidity GF.					
32764206	3	38	theme	gastrointestinal	492:507	arg1	endoscopy					509:517	upper gastrointestinal endoscopy	486:517	upper gastrointestinal endoscopy	486:517	METHODS GF and saliva samples were obtained from 158 outpatients who were undergoing upper gastrointestinal endoscopy and 36 volunteers using a nasogastric tube.					
32764206	8	39	dep	=	1133:1133	arg1	0.872					1135:1139	0.872	1135:1139	0.872	1135:1139	Spearman test also demonstrated a close correlation between pH and LPS in those samples (r = 0.872).					
32764206	4	40	theme	assay	596:600	arg1	C					636:636	recombinant Factor C	617:636	recombinant Factor C	617:636	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	4	40	theme	assay	596:600	arg1	kits					602:605	assay kits	596:605	assay kits including recombinant Factor C or Limulus amebocyte lysate	596:664	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	4	40	theme	assay	596:600	arg1	lysate					659:664	Limulus amebocyte lysate	641:664	Limulus amebocyte lysate	641:664	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	5	41	theme	operational	745:755	arg1	unit					767:770	a 16S ribosomal DNA-based operational taxonomic unit	719:770	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	5	42	theme	bacterial	681:689	arg1	composition					691:701	the bacterial composition	677:701	the bacterial composition in the samples	677:716	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	10	43	from	same	1380:1383	arg1	addition					1284:1291	addition	1284:1291	addition	1284:1291	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	10	43	from	same	1380:1383	arg1	subjects					1301:1308	the subjects	1297:1308	the subjects whose GF acidity was weak	1297:1334	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	4	44	theme	recombinant	617:627	arg1	C					636:636	recombinant Factor C	617:636	recombinant Factor C	617:636	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	5	45	theme	taxonomic	757:765	arg1	unit					767:770	a 16S ribosomal DNA-based operational taxonomic unit	719:770	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	9	46	theme	strong-acidity	1262:1275	arg1	GF					1277:1278	strong-acidity GF	1262:1278	strong-acidity GF	1262:1278	The relative abundance of GNB in the saliva showed no significant difference between the subject groups with weak- and strong-acidity GF.					
32764206	10	47	from	addition	1284:1291	arg1	abundance					1345:1353	the GNB abundance	1337:1353	the GNB abundance in the GF	1337:1363	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	10	47	from	addition	1284:1291	arg1	same					1380:1383	same	1380:1383	same	1380:1383	In addition, in the subjects whose GF acidity was weak, the GNB abundance in the GF was almost the same as that in the saliva.					
32764206	3	48	theme	saliva	416:421	arg1	samples					423:429	saliva samples	416:429	saliva samples	416:429	METHODS GF and saliva samples were obtained from 158 outpatients who were undergoing upper gastrointestinal endoscopy and 36 volunteers using a nasogastric tube.					
32764206	5	49	theme	unit	767:770	arg1	analysis					772:779	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	7	50	theme	little/no	970:978	arg1	activity					980:987	little/no activity	970:987	little/no activity in those with strong acidity (approximately < pH 2)	970:1039	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	5	51	theme	16S	721:723	arg1	unit					767:770	a 16S ribosomal DNA-based operational taxonomic unit	719:770	a 16S ribosomal DNA-based operational taxonomic unit analysis	719:779	To assess the bacterial composition in the samples, a 16S ribosomal DNA-based operational taxonomic unit analysis was performed.					
32764206	3	52	dep	METHODS	401:407	arg1	GF					409:410	GF	409:410	GF	409:410	METHODS GF and saliva samples were obtained from 158 outpatients who were undergoing upper gastrointestinal endoscopy and 36 volunteers using a nasogastric tube.					
32764206	3	52	dep	METHODS	401:407	arg1	samples					423:429	saliva samples	416:429	saliva samples	416:429	METHODS GF and saliva samples were obtained from 158 outpatients who were undergoing upper gastrointestinal endoscopy and 36 volunteers using a nasogastric tube.					
32764206	9	53	theme	significant	1197:1207	arg1	difference					1209:1218	no significant difference	1194:1218	no significant difference between the subject groups	1194:1245	The relative abundance of GNB in the saliva showed no significant difference between the subject groups with weak- and strong-acidity GF.					
32764206	12	54	theme	weak	1667:1670	arg1	acidity					1672:1678	weak acidity	1667:1678	weak acidity	1667:1678	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	12	55	from	activity	1640:1647	arg1	stomach					1654:1660	a stomach	1652:1660	a stomach with weak acidity	1652:1678	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	9	56	theme	GNB	1169:1171	arg1	abundance					1156:1164	The relative abundance	1143:1164	The relative abundance of GNB in the saliva	1143:1185	The relative abundance of GNB in the saliva showed no significant difference between the subject groups with weak- and strong-acidity GF.					
32764206	12	57	theme	prominent	1626:1634	arg1	activity					1640:1647	the prominent LPS activity	1622:1647	the prominent LPS activity in a stomach with weak acidity	1622:1678	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	1	58	theme	INTRODUCTION	120:131	arg1	Lipopolysaccharides					133:151	INTRODUCTION Lipopolysaccharides	120:151	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB)	120:190	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB) are highly toxic and induce inflammation.					
32764206	1	58	theme	INTRODUCTION	120:131	arg1	LPSs					154:157	LPSs	154:157	LPSs	154:157	INTRODUCTION Lipopolysaccharides (LPSs) of Gram-negative bacteria (GNB) are highly toxic and induce inflammation.					
32764206	7	59	theme	LPS	889:891	arg1	activity					893:900	a high LPS activity	882:900	a high LPS activity in the GF samples	882:918	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	9	60	theme	relative	1147:1154	arg1	abundance					1156:1164	The relative abundance	1143:1164	The relative abundance of GNB in the saliva	1143:1185	The relative abundance of GNB in the saliva showed no significant difference between the subject groups with weak- and strong-acidity GF.					
32764206	2	61	theme	gastric	313:319	arg1	fluid					321:325	gastric fluid	313:325	the gastric fluid (GF)	309:330	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	2	61	theme	gastric	313:319	arg1	GF					328:329	GF	328:329	GF	328:329	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	12	62	theme	DISCUSSION	1543:1552	arg1	GNB					1554:1556	DISCUSSION GNB	1543:1556	DISCUSSION GNB that have recently moved from the oral cavity	1543:1602	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	4	63	theme	amebocyte	649:657	arg1	lysate					659:664	Limulus amebocyte lysate	641:664	Limulus amebocyte lysate	641:664	The LPS activity was measured by assay kits including recombinant Factor C or Limulus amebocyte lysate.					
32764206	7	64	from	activity	893:900	arg1	samples					912:918	the GF samples	905:918	the GF samples	905:918	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	0	65	theme	Bacteria	78:85	arg1	Increase					0:7	Increase	0:7	Increase in the Lipopolysaccharide Activity	0:42	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	0	65	theme	Bacteria	78:85	arg1	Accumulation					48:59	Accumulation	48:59	Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity	48:117	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	2	66	theme	them	368:371	arg1	toxicity					356:363	the potential toxicity	342:363	the potential toxicity of them accumulated in the stomach	342:398	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	2	67	from	activity	274:281	arg1	fluid					321:325	gastric fluid	313:325	the gastric fluid (GF)	309:330	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	2	67	from	activity	274:281	arg1	GF					328:329	GF	328:329	GF	328:329	Therefore, we investigated both the LPS activity and composition of GNB in the gastric fluid (GF) to assess the potential toxicity of them accumulated in the stomach.					
32764206	0	68	theme	Gram-Negative	64:76	arg1	Bacteria					78:85	Gram-Negative Bacteria	64:85	Gram-Negative Bacteria	64:85	Increase in the Lipopolysaccharide Activity and Accumulation of Gram-Negative Bacteria in the Stomach With Low Acidity.					
32764206	8	69	theme	=	1133:1133	arg1	r					1131:1131	r	1131:1131	r = 0.872	1131:1139	Spearman test also demonstrated a close correlation between pH and LPS in those samples (r = 0.872).					
32764206	12	70	with	stomach	1654:1660	arg1	acidity					1672:1678	weak acidity	1667:1678	weak acidity	1667:1678	DISCUSSION GNB that have recently moved from the oral cavity might account for the prominent LPS activity in a stomach with weak acidity.					
32764206	7	71	theme	weak	925:928	arg1	pH					955:956	approximately > pH 4	939:958	approximately > pH 4	939:958	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	7	71	theme	weak	925:928	arg1	acidity					930:936	weak acidity	925:936	weak acidity (approximately > pH 4)	925:959	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
32764206	3	72	theme	upper	486:490	arg1	endoscopy					509:517	upper gastrointestinal endoscopy	486:517	upper gastrointestinal endoscopy	486:517	METHODS GF and saliva samples were obtained from 158 outpatients who were undergoing upper gastrointestinal endoscopy and 36 volunteers using a nasogastric tube.					
32764206	7	73	from	activity	980:987	arg1	those					992:996	those	992:996	those	992:996	RESULTS We found a high LPS activity in the GF samples with weak acidity (approximately > pH 4), whereas little/no activity in those with strong acidity (approximately < pH 2).					
34500424	4	0	theme	environmental	1128:1140	arg1	4-NP					1148:1151	the common environmental toxin 4-NP	1117:1151	the common environmental toxin 4-NP	1117:1151	Consequently, the composite hydrogel achieved an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP.					
34500424	3	1	theme	active	835:840	arg1	sites					842:846	the active sites	831:846	the active sites	831:846	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	1	2	theme	Phenolic	172:179	arg1	materials					220:228	important industrial raw materials	195:228	important industrial raw materials for various industrial applications	195:264	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	1	2	theme	Phenolic	172:179	arg1	compounds					181:189	Phenolic compounds	172:189	Phenolic compounds	172:189	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	3	3	theme	components	1015:1024	arg1	crosslinking					965:976	the chemical crosslinking	952:976	the chemical crosslinking	952:976	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	3	theme	components	1015:1024	arg1	properties					938:947	excellent mechanical properties	917:947	excellent mechanical properties	917:947	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	6	4	theme	adsorption	1428:1437	arg1	combination					1405:1415	a combination	1403:1415	a combination of surface adsorption and intra-particle diffusion mechanisms	1403:1477	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	4	5	theme	common	1121:1126	arg1	4-NP					1148:1151	the common environmental toxin 4-NP	1117:1151	the common environmental toxin 4-NP	1117:1151	Consequently, the composite hydrogel achieved an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP.					
34500424	8	6	theme	great	1729:1733	arg1	potential					1735:1743	great potential	1729:1743	great potential	1729:1743	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	6	7	theme	mechanisms	1468:1477	arg1	combination					1405:1415	a combination	1403:1415	a combination of surface adsorption and intra-particle diffusion mechanisms	1403:1477	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	3	8	from	modification	631:642	arg1	oxide					660:664	the graphene oxide	647:664	the graphene oxide (GO)	647:669	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	2	9	theme	graphene	411:418	arg1	GO-CTES-β-CD/PNIPAM					497:515	GO-CTES-β-CD/PNIPAM	497:515	GO-CTES-β-CD/PNIPAM	497:515	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	2	9	theme	graphene	411:418	arg1	oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly					420:470	a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly	383:470	a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM)	383:516	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	2	9	theme	graphene	411:418	arg1	N-isopropylacrylamide					473:493	N-isopropylacrylamide	473:493	N-isopropylacrylamide	473:493	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	3	10	dep	oxide	660:664	arg1	GO					667:668	GO	667:668	GO	667:668	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	11	theme	chemical	956:963	arg1	crosslinking					965:976	the chemical crosslinking	952:976	the chemical crosslinking	952:976	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	11	theme	chemical	956:963	arg1	properties					938:947	excellent mechanical properties	917:947	excellent mechanical properties	917:947	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	6	12	theme	diffusion	1458:1466	arg1	mechanisms					1468:1477	intra-particle diffusion mechanisms	1443:1477	intra-particle diffusion mechanisms	1443:1477	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	5	13	theme	adsorption-desorption	1174:1194	arg1	cycles					1196:1201	five repeated adsorption-desorption cycles	1160:1201	five repeated adsorption-desorption cycles	1160:1201	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	6	14	theme	multilayer	1357:1366	arg1	adsorption					1368:1377	heterogeneous multilayer adsorption	1343:1377	heterogeneous multilayer adsorption	1343:1377	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	1	15	theme	significant	308:318	arg1	hazards					349:355	significant environmental and biological hazards	308:355	significant environmental and biological hazards	308:355	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	4	16	theme	adsorption	1076:1085	arg1	capacity					1087:1094	an adsorption capacity	1073:1094	an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP	1073:1151	Consequently, the composite hydrogel achieved an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP.					
34500424	5	17	theme	initial	1237:1243	arg1	ratio					1258:1262	the initial 4-NP removal ratio	1233:1262	the initial 4-NP removal ratio	1233:1262	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	6	18	theme	intra-particle	1443:1456	arg1	mechanisms					1468:1477	intra-particle diffusion mechanisms	1443:1477	intra-particle diffusion mechanisms	1443:1477	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	6	19	theme	heterogeneous	1343:1355	arg1	adsorption					1368:1377	heterogeneous multilayer adsorption	1343:1377	heterogeneous multilayer adsorption	1343:1377	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	7	20	from	application	1535:1545	arg1	field					1554:1558	the field	1550:1558	the field of recycling phenols from wastewater	1550:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	1	21	theme	environmental	320:332	arg1	hazards					349:355	significant environmental and biological hazards	308:355	significant environmental and biological hazards	308:355	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	0	22	theme	adsorbent	127:135	arg1	hydrogel					103:110	hydrogel	103:110	hydrogel	103:110	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	0	22	theme	adsorbent	127:135	arg1	recovery					118:125	an recovery	115:125	an recovery adsorbent for phenol and microfluidic valve	115:169	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	3	23	theme	Double	618:623	arg1	modification					631:642	Double graft modification	618:642	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD)	618:805	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	7	24	from	wastewater	1586:1595	arg1	field					1554:1558	the field	1550:1558	the field of recycling phenols from wastewater	1550:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	7	24	from	wastewater	1586:1595	arg1	phenols					1573:1579	recycling phenols	1563:1579	recycling phenols from wastewater	1563:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	7	25	theme	nanocomposite	1496:1508	arg1	hydrogel					1510:1517	the nanocomposite hydrogel	1492:1517	the nanocomposite hydrogel	1492:1517	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	3	26	theme	sites	842:846	arg1	loss					823:826	the loss	819:826	the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM)	819:891	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	27	from	loss	823:826	arg1	NIPAM					886:890	NIPAM	886:890	NIPAM	886:890	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	27	from	loss	823:826	arg1	N-isopropylacrylamide					863:883	N-isopropylacrylamide	863:883	N-isopropylacrylamide (NIPAM)	863:891	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	27	from	loss	823:826	arg1	GO					856:857	GO	856:857	GO	856:857	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	0	28	theme	In-situ	0:6	arg1	oxide					52:56	In-situ formed Cyclodextrin-functionalized graphene oxide	0:56	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide)	0:87	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	1	29	theme	important	195:203	arg1	materials					220:228	important industrial raw materials	195:228	important industrial raw materials for various industrial applications	195:264	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	1	29	theme	important	195:203	arg1	compounds					181:189	Phenolic compounds	172:189	Phenolic compounds	172:189	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	0	30	theme	formed	8:13	arg1	oxide					52:56	In-situ formed Cyclodextrin-functionalized graphene oxide	0:56	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide)	0:87	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	5	31	theme	ratio	1258:1262	arg1	%					1228:1228	74%	1226:1228	74% of the initial 4-NP removal ratio	1226:1262	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	5	31	theme	ratio	1258:1262	arg1	ratio					1258:1262	the initial 4-NP removal ratio	1233:1262	the initial 4-NP removal ratio	1233:1262	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	6	32	theme	surface	1420:1426	arg1	adsorption					1428:1437	surface adsorption	1420:1437	surface adsorption	1420:1437	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	3	33	theme	silane	679:684	arg1	CTES					730:733	the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin	675:795	CTES	730:733	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	33	theme	silane	679:684	arg1	NH-β-CD					798:804	NH-β-CD	798:804	NH-β-CD	798:804	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	5	34	theme	4-NP	1245:1248	arg1	ratio					1258:1262	the initial 4-NP removal ratio	1233:1262	the initial 4-NP removal ratio	1233:1262	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	1	35	theme	industrial	205:214	arg1	materials					220:228	important industrial raw materials	195:228	important industrial raw materials for various industrial applications	195:264	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	1	35	theme	industrial	205:214	arg1	compounds					181:189	Phenolic compounds	172:189	Phenolic compounds	172:189	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	2	36	theme	adsorbent	562:570	arg1	recovery					553:560	a phenol recovery adsorbent	544:570	a phenol recovery adsorbent	544:570	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	8	37	theme	smart	1762:1766	arg1	microvalves					1768:1778	smart microvalves	1762:1778	smart microvalves	1762:1778	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	3	38	theme	coupling	686:693	arg1	CTES					730:733	the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin	675:795	CTES	730:733	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	38	theme	coupling	686:693	arg1	NH-β-CD					798:804	NH-β-CD	798:804	NH-β-CD	798:804	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	8	39	contain	possessing	1718:1727	arg1	hydrogel					1709:1716	the hydrogel	1705:1716	the hydrogel possessing great potential to be applied in smart microvalves	1705:1778	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	8	39	contain	possessing	1718:1727	arg2	potential					1735:1743	great potential	1729:1743	great potential	1729:1743	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	1	40	theme	raw	216:218	arg1	materials					220:228	important industrial raw materials	195:228	important industrial raw materials for various industrial applications	195:264	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	1	40	theme	raw	216:218	arg1	compounds					181:189	Phenolic compounds	172:189	Phenolic compounds	172:189	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	0	41	theme	graphene	43:50	arg1	oxide					52:56	In-situ formed Cyclodextrin-functionalized graphene oxide	0:56	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide)	0:87	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	1	42	theme	biological	338:347	arg1	hazards					349:355	significant environmental and biological hazards	308:355	significant environmental and biological hazards	308:355	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	3	43	theme	2-cyanoethyltriethoxysilane	701:727	arg1	CTES					730:733	the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin	675:795	CTES	730:733	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	43	theme	2-cyanoethyltriethoxysilane	701:727	arg1	NH-β-CD					798:804	NH-β-CD	798:804	NH-β-CD	798:804	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	6	44	theme	pseudo-first-order	1297:1314	arg1	kinetics					1316:1323	pseudo-first-order kinetics	1297:1323	pseudo-first-order kinetics	1297:1323	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	2	45	theme	phenol	546:551	arg1	recovery					553:560	a phenol recovery adsorbent	544:570	a phenol recovery adsorbent	544:570	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	4	46	theme	composite	1045:1053	arg1	hydrogel					1055:1062	the composite hydrogel	1041:1062	the composite hydrogel	1041:1062	Consequently, the composite hydrogel achieved an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP.					
34500424	2	47	theme	in-situ	594:600	arg1	polymerization					602:615	in-situ polymerization	594:615	in-situ polymerization	594:615	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	3	48	theme	graft	625:629	arg1	modification					631:642	Double graft modification	618:642	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD)	618:805	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	0	49	theme	microfluidic	152:163	arg1	valve					165:169	microfluidic valve	152:169	microfluidic valve	152:169	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	6	50	theme	adsorption	1269:1278	arg1	results					1280:1286	The adsorption results	1265:1286	The adsorption results	1265:1286	The adsorption results followed pseudo-first-order kinetics, corresponding to heterogeneous multilayer adsorption, which was regulated by a combination of surface adsorption and intra-particle diffusion mechanisms.					
34500424	7	51	theme	phenols	1573:1579	arg1	field					1554:1558	the field	1550:1558	the field of recycling phenols from wastewater	1550:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	1	52	theme	various	234:240	arg1	applications					253:264	various industrial applications	234:264	various industrial applications	234:264	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	4	53	theme	toxin	1142:1146	arg1	4-NP					1148:1151	the common environmental toxin 4-NP	1117:1151	the common environmental toxin 4-NP	1117:1151	Consequently, the composite hydrogel achieved an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP.					
34500424	3	54	theme	6-tetraethylenepentamine-6-deoxy	748:779	arg1	-β-cyclodextrin					781:795	the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin	675:795	-β-cyclodextrin	781:795	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	5	55	theme	repeated	1165:1172	arg1	cycles					1196:1201	five repeated adsorption-desorption cycles	1160:1201	five repeated adsorption-desorption cycles	1160:1201	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	7	56	theme	recycling	1563:1571	arg1	phenols					1573:1579	recycling phenols	1563:1579	recycling phenols from wastewater	1563:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	1	57	theme	industrial	242:251	arg1	applications					253:264	various industrial applications	234:264	various industrial applications	234:264	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34500424	8	58	theme	temperature-sensitive	1637:1657	arg1	properties					1659:1668	temperature-sensitive properties	1637:1668	temperature-sensitive properties	1637:1668	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	7	59	from	field	1554:1558	arg1	wastewater					1586:1595	wastewater	1586:1595	wastewater	1586:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	3	60	theme	agent	695:699	arg1	CTES					730:733	the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin	675:795	CTES	730:733	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	60	theme	agent	695:699	arg1	NH-β-CD					798:804	NH-β-CD	798:804	NH-β-CD	798:804	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	5	61	theme	removal	1250:1256	arg1	ratio					1258:1262	the initial 4-NP removal ratio	1233:1262	the initial 4-NP removal ratio	1233:1262	After five repeated adsorption-desorption cycles, the hydrogel retained 74% of the initial 4-NP removal ratio.					
34500424	2	62	theme	network	403:409	arg1	GO-CTES-β-CD/PNIPAM					497:515	GO-CTES-β-CD/PNIPAM	497:515	GO-CTES-β-CD/PNIPAM	497:515	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	2	62	theme	network	403:409	arg1	oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly					420:470	a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly	383:470	a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM)	383:516	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	2	62	theme	network	403:409	arg1	N-isopropylacrylamide					473:493	N-isopropylacrylamide	473:493	N-isopropylacrylamide	473:493	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	0	63	dep	oxide	52:56	arg1	N-isopropylacrylamide					66:86	N-isopropylacrylamide	66:86	N-isopropylacrylamide	66:86	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	0	63	dep	oxide	52:56	arg1	poly					60:63	poly	60:63	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide)	0:87	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	2	64	theme	three-dimensional	385:401	arg1	GO-CTES-β-CD/PNIPAM					497:515	GO-CTES-β-CD/PNIPAM	497:515	GO-CTES-β-CD/PNIPAM	497:515	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	2	64	theme	three-dimensional	385:401	arg1	oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly					420:470	a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly	383:470	a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM)	383:516	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	2	64	theme	three-dimensional	385:401	arg1	N-isopropylacrylamide					473:493	N-isopropylacrylamide	473:493	N-isopropylacrylamide	473:493	To address these issues, a three-dimensional network graphene oxide-cyanoethyltriethoxysilane-β-cyclodextrin/poly (N-isopropylacrylamide) (GO-CTES-β-CD/PNIPAM) nanocomposite hydrogel as a phenol recovery adsorbent is prepared herein by in-situ polymerization.					
34500424	4	65	theme	131.64 mg·g-1	1099:1111	arg1	capacity					1087:1094	an adsorption capacity	1073:1094	an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP	1073:1151	Consequently, the composite hydrogel achieved an adsorption capacity of 131.64 mg·g-1 for the common environmental toxin 4-NP.					
34500424	8	66	theme	photothermal	1609:1620	arg1	conversion					1622:1631	high photothermal conversion	1604:1631	high photothermal conversion	1604:1631	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	3	67	theme	single	740:745	arg1	-β-cyclodextrin					781:795	the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin	675:795	-β-cyclodextrin	781:795	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	68	theme	excellent	917:925	arg1	crosslinking					965:976	the chemical crosslinking	952:976	the chemical crosslinking	952:976	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	68	theme	excellent	917:925	arg1	properties					938:947	excellent mechanical properties	917:947	excellent mechanical properties	917:947	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	8	69	theme	high	1604:1607	arg1	conversion					1622:1631	high photothermal conversion	1604:1631	high photothermal conversion	1604:1631	Also, high photothermal conversion and temperature-sensitive properties are also demonstrated, which makes the hydrogel possessing great potential to be applied in smart microvalves.					
34500424	3	70	theme	mechanical	927:936	arg1	crosslinking					965:976	the chemical crosslinking	952:976	the chemical crosslinking	952:976	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	3	70	theme	mechanical	927:936	arg1	properties					938:947	excellent mechanical properties	917:947	excellent mechanical properties	917:947	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	7	71	theme	promising	1525:1533	arg1	application					1535:1545	promising application	1525:1545	promising application in the field of recycling phenols from wastewater	1525:1595	In general, the nanocomposite hydrogel shows promising application in the field of recycling phenols from wastewater.					
34500424	0	72	theme	Cyclodextrin-functionalized	15:41	arg1	oxide					52:56	In-situ formed Cyclodextrin-functionalized graphene oxide	0:56	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide)	0:87	In-situ formed Cyclodextrin-functionalized graphene oxide / poly (N-isopropylacrylamide) nanocomposite hydrogel as an recovery adsorbent for phenol and microfluidic valve.					
34500424	3	73	theme	graphene	651:658	arg1	oxide					660:664	the graphene oxide	647:664	the graphene oxide (GO)	647:669	Double graft modification on the graphene oxide (GO) via the silane coupling agent 2-cyanoethyltriethoxysilane (CTES) and single (6-tetraethylenepentamine-6-deoxy)-β-cyclodextrin (NH-β-CD) compensated the loss of the active sites on both GO and N-isopropylacrylamide (NIPAM), and the hydrogel shows excellent mechanical properties as the chemical crosslinking and physical entanglement of the two components.					
34500424	1	74	theme	phenol-containing	271:287	arg1	wastewater					289:298	phenol-containing wastewater	271:298	phenol-containing wastewater	271:298	Phenolic compounds are important industrial raw materials for various industrial applications, but phenol-containing wastewater creates significant environmental and biological hazards.					
34653670	8	0	theme	control	1250:1256	arg1	composition					1229:1239	The overall glycosaminoglycan composition	1199:1239	The overall glycosaminoglycan composition of adult control and KO mice	1199:1268	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	8	0	theme	control	1250:1256	arg1	similar					1275:1281	similar	1275:1281	similar	1275:1281	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	6	1	theme	reduced	961:967	arg1	morphogenesis					990:1002	reduced intact SMG branching morphogenesis	961:1002	reduced intact SMG branching morphogenesis	961:1002	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	9	2	theme	drinking	1525:1532	arg1	behavior					1534:1541	drinking behavior	1525:1541	drinking behavior in both KO mice	1525:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	8	3	theme	KO	1262:1263	arg1	mice					1265:1268	adult control and KO mice	1244:1268	mice	1265:1268	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	2	4	dep	redundancy	345:354	arg1	exists					356:361	exists	356:361	exists	356:361	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	3	5	theme	Hs3st3b1	587:594	arg1	expression					560:569	increased expression	550:569	increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration	550:700	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	10	6	theme	progenitor	1699:1708	arg1	function					1710:1717	myoepithelial progenitor function	1685:1717	myoepithelial progenitor function	1685:1717	Understanding how 3-O-sulfation regulates myoepithelial progenitor function will be important to manipulate HS-binding growth factors to enhance tissue function and regeneration.					
34653670	3	7	theme	Hs3st3a1	574:581	arg1	expression					560:569	increased expression	550:569	increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration	550:700	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	9	8	theme	KO	1551:1552	arg1	mice					1554:1557	both KO mice	1546:1557	both KO mice	1546:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	1	9	theme	extracellular	273:285	arg1	matrix					287:292	the extracellular matrix	269:292	the extracellular matrix	269:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	3	10	theme	myoepithelial	610:622	arg1	cells					624:628	endbud and myoepithelial cells	599:628	cells	624:628	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	1	11	theme	3-O-sulfated	202:213	arg1	sulfate					223:229	3-O-sulfated heparan sulfate	202:229	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix	177:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	1	11	theme	3-O-sulfated	202:213	arg1	HS					232:233	HS	232:233	HS	232:233	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	8	12	theme	HS	1293:1294	arg1	analysis					1309:1316	HS disaccharide analysis	1293:1316	HS disaccharide analysis	1293:1316	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	8	13	theme	Hs3st3a1-/-	1372:1382	arg1	HS					1384:1385	Hs3st3a1-/- HS	1372:1385	Hs3st3a1-/- HS	1372:1385	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	7	14	theme	sulfotransferases	1148:1164	arg1	expression					1166:1175	sulfotransferases expression	1148:1175	sulfotransferases expression	1148:1175	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	3	15	theme	endbud	599:604	arg1	cells					624:628	endbud and myoepithelial cells	599:628	cells	624:628	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	9	16	theme	basal	1571:1575	arg1	hypofunction					1586:1597	basal salivary hypofunction	1571:1597	basal salivary hypofunction	1571:1597	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	6	17	theme	reduced	1008:1014	arg1	HS					1029:1030	reduced 3-O-sulfated HS	1008:1030	reduced 3-O-sulfated HS	1008:1030	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	10	18	theme	tissue	1788:1793	arg1	function					1795:1802	tissue function	1788:1802	tissue function	1788:1802	Understanding how 3-O-sulfation regulates myoepithelial progenitor function will be important to manipulate HS-binding growth factors to enhance tissue function and regeneration.					
34653670	7	19	theme	HS	1070:1071	arg1	transcription					1093:1105	HS biosynthetic enzyme transcription	1070:1105	HS biosynthetic enzyme transcription	1070:1105	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	5	20	theme	fetal	882:886	arg1	morphogenesis					899:911	impaired fetal epithelial morphogenesis	873:911	impaired fetal epithelial morphogenesis	873:911	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	8	21	theme	increased	1325:1333	arg1	N-					1335:1336	increased N-	1325:1336	increased N-	1325:1336	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	10	22	theme	growth	1762:1767	arg1	factors					1769:1775	HS-binding growth factors	1751:1775	HS-binding growth factors	1751:1775	Understanding how 3-O-sulfation regulates myoepithelial progenitor function will be important to manipulate HS-binding growth factors to enhance tissue function and regeneration.					
34653670	5	23	from	mice	863:866	arg1	glands					846:851	Salivary glands	837:851	Salivary glands from both mice	837:866	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	6	24	theme	SMG	976:978	arg1	morphogenesis					990:1002	reduced intact SMG branching morphogenesis	961:1002	reduced intact SMG branching morphogenesis	961:1002	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	4	25	theme	Hs3st3a1-/-	757:767	arg1	mice					801:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	To analyze their in vivo functions, we generated both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice, which are viable and fertile.					
34653670	7	26	theme	transcription	1093:1105	arg1	Analysis					1058:1065	Analysis	1058:1065	Analysis of HS biosynthetic enzyme transcription	1058:1105	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	1	27	theme	Heparan	130:136	arg1	3-O-sulfotransferases					146:166	Heparan sulfate 3-O-sulfotransferases	130:166	Heparan sulfate 3-O-sulfotransferases	130:166	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	1	28	from	epitopes	236:243	arg1	matrix					287:292	the extracellular matrix	269:292	the extracellular matrix	269:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	1	28	from	epitopes	236:243	arg1	surfaces					253:260	cell surfaces	248:260	cell surfaces	248:260	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	9	29	theme	adult	1400:1404	arg1	function					1415:1422	adult KO gland function	1400:1422	adult KO gland function	1400:1422	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	9	30	theme	gland	1409:1413	arg1	function					1415:1422	adult KO gland function	1400:1422	adult KO gland function	1400:1422	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	3	31	theme	Single-cell	495:505	arg1	analysis					514:521	Single-cell RNAseq analysis	495:521	Single-cell RNAseq analysis of control SMGs	495:537	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	0	32	theme	adult	99:103	arg1	gland					114:118	adult salivary gland	99:118	adult salivary gland function	99:127	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	4	33	dep	in	720:721	arg1	vivo					723:726	vivo	723:726	vivo	723:726	To analyze their in vivo functions, we generated both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice, which are viable and fertile.					
34653670	4	34	theme	Hs3st3b1-/-	773:783	arg1	mice					801:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	To analyze their in vivo functions, we generated both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice, which are viable and fertile.					
34653670	9	35	theme	secretory	1440:1448	arg1	innervation					1450:1460	normal secretory innervation	1433:1460	normal secretory innervation	1433:1460	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	2	36	theme	epithelial	450:459	arg1	signaling					466:474	epithelial FGFR signaling	450:474	epithelial FGFR signaling	450:474	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	4	37	theme	knockout	792:799	arg1	mice					801:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	To analyze their in vivo functions, we generated both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice, which are viable and fertile.					
34653670	0	38	theme	Hs3st3a1	8:15	arg1	enzymes					29:35	Hs3st3a1 or Hs3st3b1 enzymes	8:35	Hs3st3a1 or Hs3st3b1 enzymes	8:35	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	1	39	theme	sulfate	138:144	arg1	3-O-sulfotransferases					146:166	Heparan sulfate 3-O-sulfotransferases	130:166	Heparan sulfate 3-O-sulfotransferases	130:166	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	2	40	theme	Hs3st3b1	419:426	arg1	HS					437:438	Hs3st3b1 sulfated HS	419:438	Hs3st3b1 sulfated HS	419:438	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	0	41	theme	Hs3st3b1	20:27	arg1	enzymes					29:35	Hs3st3a1 or Hs3st3b1 enzymes	8:35	Hs3st3a1 or Hs3st3b1 enzymes	8:35	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	9	42	from	frequency	1512:1520	arg1	mice					1554:1557	both KO mice	1546:1557	both KO mice	1546:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	3	43	theme	RNAseq	507:512	arg1	analysis					514:521	Single-cell RNAseq analysis	495:521	Single-cell RNAseq analysis of control SMGs	495:537	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	8	44	theme	overall	1203:1209	arg1	composition					1229:1239	The overall glycosaminoglycan composition	1199:1239	The overall glycosaminoglycan composition of adult control and KO mice	1199:1268	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	8	44	theme	overall	1203:1209	arg1	similar					1275:1281	similar	1275:1281	similar	1275:1281	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	9	45	from	mice	1554:1557	arg1	frequency					1512:1520	frequency	1512:1520	frequency of drinking behavior in both KO mice	1512:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	10	46	theme	myoepithelial	1685:1697	arg1	function					1710:1717	myoepithelial progenitor function	1685:1717	myoepithelial progenitor function	1685:1717	Understanding how 3-O-sulfation regulates myoepithelial progenitor function will be important to manipulate HS-binding growth factors to enhance tissue function and regeneration.					
34653670	3	47	theme	increased	550:558	arg1	expression					560:569	increased expression	550:569	increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration	550:700	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	6	48	theme	intact	969:974	arg1	morphogenesis					990:1002	reduced intact SMG branching morphogenesis	961:1002	reduced intact SMG branching morphogenesis	961:1002	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	3	49	theme	SMGs	534:537	arg1	analysis					514:521	Single-cell RNAseq analysis	495:521	Single-cell RNAseq analysis of control SMGs	495:537	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	8	50	theme	adult	1244:1248	arg1	control					1250:1256	adult control and KO mice	1244:1268	control	1250:1256	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	7	51	from	changes	1137:1143	arg1	expression					1166:1175	sulfotransferases expression	1148:1175	sulfotransferases expression	1148:1175	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	7	51	from	changes	1137:1143	arg1	development					1186:1196	development	1186:1196	development	1186:1196	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	2	52	theme	Previous	295:302	arg1	experiments					312:322	Previous ex vivo experiments	295:322	Previous ex vivo experiments	295:322	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	9	53	theme	behavior	1534:1541	arg1	frequency					1512:1520	frequency	1512:1520	frequency of drinking behavior in both KO mice	1512:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	6	54	theme	Hs3st3b1-/-	939:949	arg1	mice					951:954	Hs3st3b1-/- mice	939:954	Hs3st3b1-/- mice	939:954	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	3	55	from	expression	560:569	arg1	cells					624:628	endbud and myoepithelial cells	599:628	cells	624:628	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	1	56	theme	sulfated	184:191	arg1	epitopes					236:243	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes	177:243	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix	177:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	8	57	theme	mice	1265:1268	arg1	composition					1229:1239	The overall glycosaminoglycan composition	1199:1239	The overall glycosaminoglycan composition of adult control and KO mice	1199:1268	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	8	57	theme	mice	1265:1268	arg1	similar					1275:1281	similar	1275:1281	similar	1275:1281	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	6	58	contain	have	956:959	arg2	HS					1029:1030	reduced 3-O-sulfated HS	1008:1030	reduced 3-O-sulfated HS	1008:1030	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	6	58	contain	have	956:959	arg1	mice					951:954	Hs3st3b1-/- mice	939:954	Hs3st3b1-/- mice	939:954	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	6	58	contain	have	956:959	arg2	morphogenesis					990:1002	reduced intact SMG branching morphogenesis	961:1002	reduced intact SMG branching morphogenesis	961:1002	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	10	59	theme	HS-binding	1751:1760	arg1	factors					1769:1775	HS-binding growth factors	1751:1775	HS-binding growth factors	1751:1775	Understanding how 3-O-sulfation regulates myoepithelial progenitor function will be important to manipulate HS-binding growth factors to enhance tissue function and regeneration.					
34653670	1	60	theme	rare	197:200	arg1	epitopes					236:243	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes	177:243	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix	177:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	8	61	theme	non-sulfated	1342:1353	arg1	disaccharides					1355:1367	non-sulfated disaccharides	1342:1367	non-sulfated disaccharides	1342:1367	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	1	62	theme	heparan	215:221	arg1	sulfate					223:229	3-O-sulfated heparan sulfate	202:229	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix	177:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	1	62	theme	heparan	215:221	arg1	HS					232:233	HS	232:233	HS	232:233	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	6	63	theme	3-O-sulfated	1016:1027	arg1	HS					1029:1030	reduced 3-O-sulfated HS	1008:1030	reduced 3-O-sulfated HS	1008:1030	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	7	64	theme	compensatory	1124:1135	arg1	changes					1137:1143	some compensatory changes	1119:1143	some compensatory changes in sulfotransferases expression early in development	1119:1196	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	5	65	theme	impaired	873:880	arg1	morphogenesis					899:911	impaired fetal epithelial morphogenesis	873:911	impaired fetal epithelial morphogenesis	873:911	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	8	66	theme	disaccharide	1296:1307	arg1	analysis					1309:1316	HS disaccharide analysis	1293:1316	HS disaccharide analysis	1293:1316	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	7	67	theme	biosynthetic	1073:1084	arg1	transcription					1093:1105	HS biosynthetic enzyme transcription	1070:1105	HS biosynthetic enzyme transcription	1070:1105	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	6	68	theme	branching	980:988	arg1	morphogenesis					990:1002	reduced intact SMG branching morphogenesis	961:1002	reduced intact SMG branching morphogenesis	961:1002	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	2	69	dep	ex	304:305	arg1	vivo					307:310	vivo	307:310	vivo	307:310	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	7	70	theme	enzyme	1086:1091	arg1	transcription					1093:1105	HS biosynthetic enzyme transcription	1070:1105	HS biosynthetic enzyme transcription	1070:1105	Analysis of HS biosynthetic enzyme transcription highlighted some compensatory changes in sulfotransferases expression early in development.					
34653670	3	71	theme	progenitor	649:658	arg1	both					631:634	both	631:634	both	631:634	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	3	71	theme	progenitor	649:658	arg1	cells					624:628	endbud and myoepithelial cells	599:628	cells	624:628	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	3	71	theme	progenitor	649:658	arg1	cells					660:664	progenitor cells	649:664	progenitor cells	649:664	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	9	72	theme	myoepithelial	1616:1628	arg1	dysfunction					1630:1640	myoepithelial dysfunction	1616:1640	myoepithelial dysfunction	1616:1640	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	4	73	theme	in	720:721	arg1	functions					728:736	their in vivo functions	714:736	their in vivo functions	714:736	To analyze their in vivo functions, we generated both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice, which are viable and fertile.					
34653670	0	74	theme	epithelial	70:79	arg1	morphogenesis					81:93	epithelial morphogenesis	70:93	epithelial morphogenesis	70:93	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	9	75	from	increase	1500:1507	arg1	frequency					1512:1520	frequency	1512:1520	frequency of drinking behavior in both KO mice	1512:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	0	76	theme	gland	114:118	arg1	function					120:127	adult salivary gland function	99:127	adult salivary gland function	99:127	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	9	77	theme	KO	1406:1407	arg1	function					1415:1422	adult KO gland function	1400:1422	adult KO gland function	1400:1422	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	2	78	theme	ex	304:305	arg1	experiments					312:322	Previous ex vivo experiments	295:322	Previous ex vivo experiments	295:322	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	6	79	theme	basement	1039:1046	arg1	membrane					1048:1055	the basement membrane	1035:1055	the basement membrane	1035:1055	Hs3st3b1-/- mice have reduced intact SMG branching morphogenesis and reduced 3-O-sulfated HS in the basement membrane.					
34653670	5	80	theme	epithelial	888:897	arg1	morphogenesis					899:911	impaired fetal epithelial morphogenesis	873:911	impaired fetal epithelial morphogenesis	873:911	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	0	81	theme	salivary	105:112	arg1	gland					114:118	adult salivary gland	99:118	adult salivary gland function	99:127	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	9	82	theme	function	1415:1422	arg1	Analysis					1388:1395	Analysis	1388:1395	Analysis of adult KO gland function	1388:1422	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	1	83	theme	sulfate	223:229	arg1	epitopes					236:243	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes	177:243	highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix	177:292	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
34653670	5	84	theme	Salivary	837:844	arg1	glands					846:851	Salivary glands	837:851	Salivary glands from both mice	837:866	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	9	85	theme	normal	1433:1438	arg1	innervation					1450:1460	normal secretory innervation	1433:1460	normal secretory innervation	1433:1460	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	2	86	theme	FGFR	461:464	arg1	signaling					466:474	epithelial FGFR signaling	450:474	epithelial FGFR signaling	450:474	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	5	87	contain	have	868:871	arg1	glands					846:851	Salivary glands	837:851	Salivary glands from both mice	837:866	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	5	87	contain	have	868:871	arg2	morphogenesis					899:911	impaired fetal epithelial morphogenesis	873:911	impaired fetal epithelial morphogenesis	873:911	Salivary glands from both mice have impaired fetal epithelial morphogenesis when cultured with FGF10.					
34653670	2	88	theme	enzymes	389:395	arg1	family					373:378	the family	369:378	the family of seven enzymes	369:395	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	9	89	from	behavior	1534:1541	arg1	mice					1554:1557	both KO mice	1546:1557	both KO mice	1546:1557	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	4	90	theme	single	785:790	arg1	mice					801:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice	752:804	To analyze their in vivo functions, we generated both Hs3st3a1-/- and Hs3st3b1-/- single knockout mice, which are viable and fertile.					
34653670	2	91	theme	sulfated	428:435	arg1	HS					437:438	Hs3st3b1 sulfated HS	419:438	Hs3st3b1 sulfated HS	419:438	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	0	92	theme	enzymes	29:35	arg1	Loss					0:3	Loss	0:3	Loss of Hs3st3a1 or Hs3st3b1 enzymes	0:35	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	9	93	theme	salivary	1577:1584	arg1	hypofunction					1586:1597	basal salivary hypofunction	1571:1597	basal salivary hypofunction	1571:1597	Analysis of adult KO gland function revealed normal secretory innervation, but without stimulation there was an increase in frequency of drinking behavior in both KO mice, suggesting basal salivary hypofunction, possibly due to myoepithelial dysfunction.					
34653670	0	94	theme	heparan	44:50	arg1	sulfate					52:58	heparan sulfate	44:58	heparan sulfate	44:58	Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to reduce epithelial morphogenesis and adult salivary gland function.					
34653670	3	95	theme	control	526:532	arg1	SMGs					534:537	control SMGs	526:537	control SMGs	526:537	Single-cell RNAseq analysis of control SMGs identifies increased expression of Hs3st3a1 and Hs3st3b1 in endbud and myoepithelial cells, both of which are progenitor cells during development and regeneration.					
34653670	8	96	theme	glycosaminoglycan	1211:1227	arg1	composition					1229:1239	The overall glycosaminoglycan composition	1199:1239	The overall glycosaminoglycan composition of adult control and KO mice	1199:1268	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	8	96	theme	glycosaminoglycan	1211:1227	arg1	similar					1275:1281	similar	1275:1281	similar	1275:1281	The overall glycosaminoglycan composition of adult control and KO mice were similar, although HS disaccharide analysis showed increased N- and non-sulfated disaccharides in Hs3st3a1-/- HS.					
34653670	2	97	theme	functional	334:343	arg1	redundancy					345:354	functional redundancy exists	334:361	functional redundancy exists among the family of seven enzymes	334:395	Previous ex vivo experiments suggested functional redundancy exists among the family of seven enzymes but that Hs3st3a1 and Hs3st3b1 sulfated HS increases epithelial FGFR signaling and morphogenesis.					
34653670	1	98	theme	cell	248:251	arg1	surfaces					253:260	cell surfaces	248:260	cell surfaces	248:260	Heparan sulfate 3-O-sulfotransferases generate highly sulfated but rare 3-O-sulfated heparan sulfate (HS) epitopes on cell surfaces and in the extracellular matrix.					
31904461	4	0	theme	feasibility	729:739	arg1	performances					626:637	performances	626:637	performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility	626:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	7	1	theme	detection	1206:1214	arg1	limit					1197:1201	the limit	1193:1201	the limit of detection (LOD)	1193:1220	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	10	2	from	analysis	1515:1522	arg1	system					1563:1568	intricate interference system	1540:1568	intricate interference system	1540:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	1	3	theme	amide	254:258	arg1	reaction					260:267	a facile amide reaction	245:267	a facile amide reaction between chitosan and graphene oxide	245:303	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	0	4	theme	effective	84:92	arg1	determination					94:106	the sensitive and effective determination	66:106	the sensitive and effective determination of alkaloids in hotpot	66:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	6	5	theme	extraction	911:920	arg1	procedure					922:930	extraction procedure	911:930	extraction procedure	911:930	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	2	6	theme	@	348:348	arg1	CS/GO					349:353	Fe3O4@SiO2@CS/GO	338:353	Fe3O4@SiO2@CS/GO	338:353	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	10	7	theme	intricate	1540:1548	arg1	system					1563:1568	intricate interference system	1540:1568	intricate interference system	1540:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	0	8	theme	alkaloids	111:119	arg1	determination					94:106	the sensitive and effective determination	66:106	the sensitive and effective determination of alkaloids in hotpot	66:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	10	9	theme	alkaloids	1527:1535	arg1	analysis					1515:1522	the analysis	1511:1522	the analysis of alkaloids in intricate interference system	1511:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	7	10	theme	optimized	1068:1076	arg1	conditions					1078:1087	the optimized conditions	1064:1087	the optimized conditions	1064:1087	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	4	11	theme	environment	695:705	arg1	friendly					707:714	environment friendly	695:714	environment friendly	695:714	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	5	12	theme	π-π	790:792	arg1	interaction					818:828	π-π electron-donor-acceptor interaction	790:828	π-π electron-donor-acceptor interaction	790:828	The adsorption mechanism for alkaloids included π-π electron-donor-acceptor interaction, cation-π interaction and hydrogen bonding.					
31904461	6	13	theme	desorption	979:988	arg1	conditions					990:999	desorption conditions	979:999	desorption conditions	979:999	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	10	14	theme	guidance	1477:1484	arg1	method					1500:1505	valuable guidance and effective method	1468:1505	valuable guidance and effective method for the analysis of alkaloids in intricate interference system	1468:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	5	15	theme	electron-donor-acceptor	794:816	arg1	interaction					818:828	π-π electron-donor-acceptor interaction	790:828	π-π electron-donor-acceptor interaction	790:828	The adsorption mechanism for alkaloids included π-π electron-donor-acceptor interaction, cation-π interaction and hydrogen bonding.					
31904461	9	16	from	detection	1414:1422	arg1	hotpot					1440:1445	hotpot	1440:1445	hotpot	1440:1445	These results indicated that the developed method was successfully applied for simultaneous detection of alkaloids in hotpot.					
31904461	4	17	theme	easy	659:662	arg1	operation					664:672	easy operation	659:672	easy operation	659:672	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	3	18	theme	nanoadsorbent	528:540	arg1	structure					511:519	structure	511:519	structure	511:519	The composition and structure of the nanoadsorbent was systematically characterized by various techniques.					
31904461	3	18	theme	nanoadsorbent	528:540	arg1	composition					495:505	composition	495:505	composition	495:505	The composition and structure of the nanoadsorbent was systematically characterized by various techniques.					
31904461	8	19	theme	standard	1284:1291	arg1	RSDs					1305:1308	RSDs	1305:1308	RSDs	1305:1308	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	8	19	theme	standard	1284:1291	arg1	deviations					1293:1302	Intra- and inter-day relative standard deviations	1254:1302	Intra- and inter-day relative standard deviations (RSDs)	1254:1309	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	1	20	theme	Fe3O4	195:199	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	20	theme	Fe3O4	195:199	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	8	21	theme	Intra-	1254:1259	arg1	RSDs					1305:1308	RSDs	1305:1308	RSDs	1305:1308	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	8	21	theme	Intra-	1254:1259	arg1	deviations					1293:1302	Intra- and inter-day relative standard deviations	1254:1302	Intra- and inter-day relative standard deviations (RSDs)	1254:1309	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	9	22	theme	simultaneous	1401:1412	arg1	detection					1414:1422	simultaneous detection	1401:1422	simultaneous detection of alkaloids in hotpot	1401:1445	These results indicated that the developed method was successfully applied for simultaneous detection of alkaloids in hotpot.					
31904461	5	23	theme	hydrogen	856:863	arg1	bonding					865:871	hydrogen bonding	856:871	hydrogen bonding	856:871	The adsorption mechanism for alkaloids included π-π electron-donor-acceptor interaction, cation-π interaction and hydrogen bonding.					
31904461	3	24	dep	composition	495:505	arg1	The					491:493	The	491:493	The	491:493	The composition and structure of the nanoadsorbent was systematically characterized by various techniques.					
31904461	1	25	theme	@	200:200	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	25	theme	@	200:200	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	2	26	theme	multiple	447:454	arg1	alkaloids					456:464	multiple alkaloids	447:464	multiple alkaloids from the complex matrix	447:488	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	6	27	theme	adsorption	962:971	arg1	time					973:976	adsorption time	962:976	adsorption time	962:976	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	1	28	theme	SiO2	201:204	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	28	theme	SiO2	201:204	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	8	29	theme	inter-day	1265:1273	arg1	RSDs					1305:1308	RSDs	1305:1308	RSDs	1305:1308	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	8	29	theme	inter-day	1265:1273	arg1	deviations					1293:1302	Intra- and inter-day relative standard deviations	1254:1302	Intra- and inter-day relative standard deviations (RSDs)	1254:1309	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	10	30	from	alkaloids	1527:1535	arg1	system					1563:1568	intricate interference system	1540:1568	intricate interference system	1540:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	10	31	theme	interference	1550:1561	arg1	system					1563:1568	intricate interference system	1540:1568	intricate interference system	1540:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	2	32	theme	extraction	314:323	arg1	method					325:330	A novel extraction method	306:330	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent	306:370	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	1	33	theme	@	205:205	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	33	theme	@	205:205	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	0	34	theme	graphene	33:40	arg1	nanocomposite					48:60	magnetic graphene oxide nanocomposite	24:60	magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot	24:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	1	35	theme	graphene	290:297	arg1	oxide					299:303	graphene oxide	290:303	graphene oxide	290:303	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	5	36	theme	adsorption	746:755	arg1	mechanism					757:765	The adsorption mechanism	742:765	The adsorption mechanism for alkaloids	742:779	The adsorption mechanism for alkaloids included π-π electron-donor-acceptor interaction, cation-π interaction and hydrogen bonding.					
31904461	6	37	theme	principal	878:886	arg1	regeneration					1005:1016	regeneration	1005:1016	regeneration	1005:1016	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	6	37	theme	principal	878:886	arg1	time					973:976	adsorption time	962:976	adsorption time	962:976	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	6	37	theme	principal	878:886	arg1	parameters					888:897	The principal parameters	874:897	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles	874:1023	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	6	37	theme	principal	878:886	arg1	dosage					950:955	adsorbent dosage	940:955	adsorbent dosage	940:955	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	6	37	theme	principal	878:886	arg1	pH					958:959	pH	958:959	pH	958:959	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	6	37	theme	principal	878:886	arg1	conditions					990:999	desorption conditions	979:999	desorption conditions	979:999	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	2	38	theme	novel	308:312	arg1	method					325:330	A novel extraction method	306:330	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent	306:370	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	10	39	theme	effective	1490:1498	arg1	method					1500:1505	valuable guidance and effective method	1468:1505	valuable guidance and effective method for the analysis of alkaloids in intricate interference system	1468:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	4	40	theme	operation	664:672	arg1	performances					626:637	performances	626:637	performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility	626:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	0	41	theme	magnetic	24:31	arg1	nanocomposite					48:60	magnetic graphene oxide nanocomposite	24:60	magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot	24:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	6	42	theme	adsorbent	940:948	arg1	dosage					950:955	adsorbent dosage	940:955	adsorbent dosage	940:955	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	7	43	theme	higher	1171:1176	arg1	r2					1167:1168	r2	1167:1168	r2	1167:1168	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	7	43	theme	higher	1171:1176	arg1	coefficient					1154:1164	correlation coefficient	1142:1164	correlation coefficient (r2) higher than 0.997	1142:1187	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	0	44	from	determination	94:106	arg1	hotpot					124:129	hotpot	124:129	hotpot	124:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	3	45	theme	various	578:584	arg1	techniques					586:595	various techniques	578:595	various techniques	578:595	The composition and structure of the nanoadsorbent was systematically characterized by various techniques.					
31904461	2	46	theme	efficient	405:413	arg1	extraction					415:424	efficient extraction	405:424	efficient extraction	405:424	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	47	theme	complex	475:481	arg1	matrix					483:488	the complex matrix	471:488	the complex matrix	471:488	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	48	theme	@	343:343	arg1	CS/GO					349:353	Fe3O4@SiO2@CS/GO	338:353	Fe3O4@SiO2@CS/GO	338:353	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	49	from	matrix	483:488	arg1	determination					430:442	determination	430:442	determination	430:442	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	49	from	matrix	483:488	arg1	alkaloids					456:464	multiple alkaloids	447:464	multiple alkaloids from the complex matrix	447:488	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	49	from	matrix	483:488	arg1	extraction					415:424	efficient extraction	405:424	efficient extraction	405:424	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	4	50	contain	possesses	616:624	arg2	performances					626:637	performances	626:637	performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility	626:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	4	50	contain	possesses	616:624	arg1	nanoadsorbent					602:614	The nanoadsorbent	598:614	The nanoadsorbent	598:614	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	4	51	theme	friendly	707:714	arg1	performances					626:637	performances	626:637	performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility	626:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	4	52	theme	superparamagnetism	675:692	arg1	performances					626:637	performances	626:637	performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility	626:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	0	53	theme	oxide	42:46	arg1	nanocomposite					48:60	magnetic graphene oxide nanocomposite	24:60	magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot	24:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	10	54	from	system	1563:1568	arg1	analysis					1515:1522	the analysis	1511:1522	the analysis of alkaloids in intricate interference system	1511:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	9	55	theme	alkaloids	1427:1435	arg1	detection					1414:1422	simultaneous detection	1401:1422	simultaneous detection of alkaloids in hotpot	1401:1445	These results indicated that the developed method was successfully applied for simultaneous detection of alkaloids in hotpot.					
31904461	7	56	theme	linear	1116:1121	arg1	range					1131:1135	good linear dynamic range	1111:1135	good linear dynamic range with correlation coefficient (r2) higher than 0.997	1111:1187	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	8	57	theme	relative	1275:1282	arg1	RSDs					1305:1308	RSDs	1305:1308	RSDs	1305:1308	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	8	57	theme	relative	1275:1282	arg1	deviations					1293:1302	Intra- and inter-day relative standard deviations	1254:1302	Intra- and inter-day relative standard deviations (RSDs)	1254:1309	Intra- and inter-day relative standard deviations (RSDs) were <10%.					
31904461	6	58	dep	dosage	950:955	arg1	cycles					1018:1023	cycles	1018:1023	cycles	1018:1023	The principal parameters influencing extraction procedure such as adsorbent dosage, pH, adsorption time, desorption conditions and regeneration cycles were investigated and optimized.					
31904461	7	59	theme	correlation	1142:1152	arg1	r2					1167:1168	r2	1167:1168	r2	1167:1168	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	7	59	theme	correlation	1142:1152	arg1	coefficient					1154:1164	correlation coefficient	1142:1164	correlation coefficient (r2) higher than 0.997	1142:1187	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	5	60	theme	cation-π	831:838	arg1	interaction					840:850	cation-π interaction	831:850	cation-π interaction	831:850	The adsorption mechanism for alkaloids included π-π electron-donor-acceptor interaction, cation-π interaction and hydrogen bonding.					
31904461	2	61	dep	extraction	415:424	arg1	the					401:403	the	401:403	the	401:403	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	7	62	theme	dynamic	1123:1129	arg1	range					1131:1135	good linear dynamic range	1111:1135	good linear dynamic range with correlation coefficient (r2) higher than 0.997	1111:1187	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	2	63	theme	SiO2	344:347	arg1	CS/GO					349:353	Fe3O4@SiO2@CS/GO	338:353	Fe3O4@SiO2@CS/GO	338:353	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	64	theme	alkaloids	456:464	arg1	determination					430:442	determination	430:442	determination	430:442	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	2	64	theme	alkaloids	456:464	arg1	extraction					415:424	efficient extraction	405:424	efficient extraction	405:424	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	9	65	theme	developed	1355:1363	arg1	method					1365:1370	the developed method	1351:1370	the developed method	1351:1370	These results indicated that the developed method was successfully applied for simultaneous detection of alkaloids in hotpot.					
31904461	0	66	from	hotpot	124:129	arg1	determination					94:106	the sensitive and effective determination	66:106	the sensitive and effective determination of alkaloids in hotpot	66:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	1	67	theme	Chitosan	132:139	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	67	theme	Chitosan	132:139	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	4	68	theme	economic	720:727	arg1	feasibility					729:739	economic feasibility	720:739	economic feasibility	720:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	1	69	theme	functionalized	141:154	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	69	theme	functionalized	141:154	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	7	70	with	range	1131:1135	arg1	r2					1167:1168	r2	1167:1168	r2	1167:1168	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	7	70	with	range	1131:1135	arg1	coefficient					1154:1164	correlation coefficient	1142:1164	correlation coefficient (r2) higher than 0.997	1142:1187	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	4	71	theme	high	642:645	arg1	efficiency					647:656	high efficiency	642:656	high efficiency	642:656	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	7	72	theme	good	1111:1114	arg1	range					1131:1135	good linear dynamic range	1111:1135	good linear dynamic range with correlation coefficient (r2) higher than 0.997	1111:1187	Under the optimized conditions, the method exhibited good linear dynamic range with correlation coefficient (r2) higher than 0.997 and the limit of detection (LOD) was among 0.016--0.092 μg kg-1.					
31904461	0	73	from	alkaloids	111:119	arg1	hotpot					124:129	hotpot	124:129	hotpot	124:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	1	74	theme	magnetic	156:163	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	74	theme	magnetic	156:163	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	2	75	theme	Fe3O4	338:342	arg1	CS/GO					349:353	Fe3O4@SiO2@CS/GO	338:353	Fe3O4@SiO2@CS/GO	338:353	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	1	76	theme	graphene	165:172	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	76	theme	graphene	165:172	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	10	77	theme	valuable	1468:1475	arg1	method					1500:1505	valuable guidance and effective method	1468:1505	valuable guidance and effective method for the analysis of alkaloids in intricate interference system	1468:1568	This study provided valuable guidance and effective method for the analysis of alkaloids in intricate interference system.					
31904461	0	78	theme	sensitive	70:78	arg1	determination					94:106	the sensitive and effective determination	66:106	the sensitive and effective determination of alkaloids in hotpot	66:129	Chitosan functionalized magnetic graphene oxide nanocomposite for the sensitive and effective determination of alkaloids in hotpot.					
31904461	2	79	from	determination	430:442	arg1	matrix					483:488	the complex matrix	471:488	the complex matrix	471:488	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	4	80	theme	efficiency	647:656	arg1	performances					626:637	performances	626:637	performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility	626:739	The nanoadsorbent possesses performances of high efficiency, easy operation, superparamagnetism, environment friendly and economic feasibility.					
31904461	2	81	from	extraction	415:424	arg1	matrix					483:488	the complex matrix	471:488	the complex matrix	471:488	A novel extraction method using Fe3O4@SiO2@CS/GO as nanoadsorbent was developed and applied to the efficient extraction and determination of multiple alkaloids from the complex matrix.					
31904461	1	82	theme	oxide	174:178	arg1	nanocomposite					180:192	Chitosan functionalized magnetic graphene oxide nanocomposite	132:192	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO)	132:211	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	82	theme	oxide	174:178	arg1	CS/GO					206:210	Fe3O4@SiO2@CS/GO	195:210	Fe3O4@SiO2@CS/GO	195:210	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
31904461	1	83	theme	facile	247:252	arg1	reaction					260:267	a facile amide reaction	245:267	a facile amide reaction between chitosan and graphene oxide	245:303	Chitosan functionalized magnetic graphene oxide nanocomposite (Fe3O4@SiO2@CS/GO) was successfully fabricated via a facile amide reaction between chitosan and graphene oxide.					
32920435	4	0	theme	growth	942:947	arg1	activity					960:967	the effective growth inhibitory activity	928:967	the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers	928:1022	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	5	1	theme	tumor	1119:1123	arg1	cells					1125:1129	eight tumor cells	1113:1129	eight tumor cells	1113:1129	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	0	2	theme	bacterial	169:177	arg1	membranes					189:197	bacterial cellulose membranes	169:197	bacterial cellulose membranes	169:197	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	2	3	theme	coordination	694:705	arg1	C7H7NO2					720:726	the coordination formula [Ag (C7H7NO2)2	690:728	the coordination formula [Ag (C7H7NO2)2(NO3)]	690:734	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	2	3	theme	coordination	694:705	arg1	NO3					730:732	NO3	730:732	NO3	730:732	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	4	theme	tract	1665:1669	arg1	infections					1671:1680	urinary tract infections	1657:1680	urinary tract infections	1657:1680	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	3	5	theme	silver	769:774	arg1	ion					776:778	the silver ion	765:778	the silver ion	765:778	The coordination of pABA to the silver ion occurred by the nitrogen atom.					
32920435	4	6	theme	effective	932:940	arg1	activity					960:967	the effective growth inhibitory activity	928:967	the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers	928:1022	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	5	7	theme	cells	1125:1129	arg1	panel					1104:1108	a panel	1102:1108	a panel of eight tumor cells	1102:1129	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	4	8	theme	Gram-positive	977:989	arg1	producers					1014:1022	Gram-positive, Gram-negative biofilm producers	977:1022	Gram-positive, Gram-negative biofilm producers	977:1022	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	2	9	theme	1:2	597:599	arg1	composition					612:622	a 1:2 metal/pABA composition	595:622	a 1:2 metal/pABA composition	595:622	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	0	10	theme	complex	91:97	arg1	panel					73:77	a tumor cells panel	59:77	a tumor cells panel of a silver complex with 4-aminobenzoic acid	59:122	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	1	11	theme	4-aminobenzoic	335:348	arg1	Ag-pABA					356:362	Ag-pABA	356:362	Ag-pABA	356:362	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	1	11	theme	4-aminobenzoic	335:348	arg1	acid					350:353	4-aminobenzoic acid	335:353	4-aminobenzoic acid (Ag-pABA)	335:363	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	4	12	theme	in	815:816	arg1	activity					838:845	The in vitro antibacterial activity	811:845	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays	811:913	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	7	13	theme	sustained	1358:1366	arg1	release					1368:1374	sustained release	1358:1374	sustained release of Ag-pABA	1358:1385	The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA.					
32920435	1	14	with	complex	322:328	arg1	Ag-pABA					356:362	Ag-pABA	356:362	Ag-pABA	356:362	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	1	14	with	complex	322:328	arg1	system					385:390	a drug delivery system	369:390	a drug delivery system based on bacterial cellulose (BC-Ag-pABA)	369:432	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	1	14	with	complex	322:328	arg1	acid					350:353	4-aminobenzoic acid	335:353	4-aminobenzoic acid (Ag-pABA)	335:363	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	0	15	theme	silver	84:89	arg1	complex					91:97	a silver complex	82:97	a silver complex with 4-aminobenzoic acid	82:122	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	0	16	theme	cellulose	179:187	arg1	membranes					189:197	bacterial cellulose membranes	169:197	bacterial cellulose membranes	169:197	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	6	17	theme	Ames	1252:1255	arg1	Test					1257:1260	the Ames Test	1248:1260	the Ames Test	1248:1260	The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity.					
32920435	4	18	theme	inhibitory	949:958	arg1	activity					960:967	the effective growth inhibitory activity	928:967	the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers	928:1022	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	0	19	theme	4-aminobenzoic	104:117	arg1	acid					119:122	4-aminobenzoic acid	104:122	4-aminobenzoic acid	104:122	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	8	20	theme	biological	1506:1515	arg1	effects					1517:1523	the in vivo biological effects	1494:1523	the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer	1494:1691	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	2	21	theme	nitrate	631:637	arg1	ion					639:641	a nitrate ion	629:641	a nitrate ion	629:641	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	22	theme	action	1455:1460	arg1	conditions					1470:1479	the conditions	1466:1479	the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer	1466:1691	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	8	22	theme	action	1455:1460	arg1	mechanisms					1441:1450	its mechanisms	1437:1450	its mechanisms of action	1437:1460	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	6	23	theme	interaction	1223:1233	arg1	capacity					1235:1242	The DNA interaction capacity	1215:1242	The DNA interaction capacity	1215:1242	The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity.					
32920435	4	24	theme	concentration	894:906	arg1	assays					908:913	minimum inhibitory concentration assays	875:913	minimum inhibitory concentration assays	875:913	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	8	25	theme	animal	1531:1536	arg1	models					1538:1543	animal models	1531:1543	animal models	1531:1543	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	5	26	theme	selectivity	1191:1201	arg1	SI					1210:1211	SI	1210:1211	SI	1210:1211	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	5	26	theme	selectivity	1191:1201	arg1	index					1203:1207	a significant selectivity index	1177:1207	a significant selectivity index (SI)	1177:1212	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	2	27	theme	oxygen	672:677	arg1	atom					679:682	the oxygen atom	668:682	the oxygen atom	668:682	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	3	28	theme	pABA	757:760	arg1	coordination					741:752	The coordination	737:752	The coordination of pABA to the silver ion	737:778	The coordination of pABA to the silver ion occurred by the nitrogen atom.					
32920435	8	29	theme	skin	1602:1605	arg1	tissues					1616:1622	skin and soft tissues	1602:1622	tissues	1616:1622	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	5	30	theme	complex	1164:1170	arg1	activity					1148:1155	the activity	1144:1155	the activity of the complex	1144:1170	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	4	31	theme	inhibitory	883:892	arg1	concentration					894:906	minimum inhibitory concentration	875:906	minimum inhibitory concentration assays	875:913	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	6	32	theme	mutagenicity	1287:1298	arg1	absence					1276:1282	the absence	1272:1282	the absence of mutagenicity	1272:1298	The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity.					
32920435	0	33	theme	Antibacterial	0:12	arg1	activities					14:23	Antibacterial activities	0:23	Antibacterial activities	0:23	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	1	34	theme	antibacterial	254:266	arg1	potential					290:298	the antibacterial and antiproliferative potential	250:298	the antibacterial and antiproliferative potential	250:298	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	2	35	theme	metal/pABA	601:610	arg1	composition					612:622	a 1:2 metal/pABA composition	595:622	a 1:2 metal/pABA composition	595:622	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	0	36	with	complex	91:97	arg1	acid					119:122	4-aminobenzoic acid	104:122	4-aminobenzoic acid	104:122	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	7	37	theme	BC-Ag-pABA	1305:1314	arg1	composite					1316:1324	The BC-Ag-pABA composite	1301:1324	The BC-Ag-pABA composite	1301:1324	The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA.					
32920435	0	38	theme	antiproliferative	29:45	arg1	assays					47:52	antiproliferative assays	29:52	antiproliferative assays	29:52	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	2	39	theme	single-crystal	534:547	arg1	diffraction					555:565	single-crystal X-ray diffraction	534:565	single-crystal X-ray diffraction	534:565	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	2	40	theme	X-ray	549:553	arg1	diffraction					555:565	single-crystal X-ray diffraction	534:565	single-crystal X-ray diffraction	534:565	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	41	from	studies	1426:1432	arg1	conditions					1470:1479	the conditions	1466:1479	the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer	1466:1691	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	8	41	from	studies	1426:1432	arg1	mechanisms					1441:1450	its mechanisms	1437:1450	its mechanisms of action	1437:1460	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	4	42	theme	biofilm	1006:1012	arg1	producers					1014:1022	Gram-positive, Gram-negative biofilm producers	977:1022	Gram-positive, Gram-negative biofilm producers	977:1022	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	1	43	theme	antiproliferative	272:288	arg1	potential					290:298	the antibacterial and antiproliferative potential	250:298	the antibacterial and antiproliferative potential	250:298	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	2	44	dep	analysis	486:493	arg1	techniques					567:576	techniques	567:576	techniques	567:576	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	45	dep	in	1498:1499	arg1	vivo					1501:1504	vivo	1501:1504	vivo	1501:1504	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	4	46	dep	in	815:816	arg1	vitro					818:822	vitro	818:822	vitro	818:822	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	5	47	theme	significant	1179:1189	arg1	SI					1210:1211	SI	1210:1211	SI	1210:1211	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	5	47	theme	significant	1179:1189	arg1	index					1203:1207	a significant selectivity index	1177:1207	a significant selectivity index (SI)	1177:1212	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	4	48	theme	minimum	875:881	arg1	concentration					894:906	minimum inhibitory concentration	875:906	minimum inhibitory concentration assays	875:913	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	6	49	theme	DNA	1219:1221	arg1	capacity					1235:1242	The DNA interaction capacity	1215:1242	The DNA interaction capacity	1215:1242	The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity.					
32920435	2	50	theme	Ag-pABA	439:445	arg1	complex					447:453	The Ag-pABA complex	435:453	The Ag-pABA complex	435:453	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	2	51	theme	mass	512:515	arg1	spectrometry					517:528	high resolution mass spectrometry	496:528	high resolution mass spectrometry	496:528	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	1	52	theme	drug	371:374	arg1	system					385:390	a drug delivery system	369:390	a drug delivery system based on bacterial cellulose (BC-Ag-pABA)	369:432	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	7	53	theme	Ag-pABA	1379:1385	arg1	release					1368:1374	sustained release	1358:1374	sustained release of Ag-pABA	1358:1385	The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA.					
32920435	0	54	theme	tumor	61:65	arg1	cells					67:71	tumor cells	61:71	a tumor cells panel of a silver complex with 4-aminobenzoic acid	59:122	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	2	55	theme	resolution	501:510	arg1	spectrometry					517:528	high resolution mass spectrometry	496:528	high resolution mass spectrometry	496:528	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	4	56	theme	antibacterial	824:836	arg1	activity					838:845	The in vitro antibacterial activity	811:845	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays	811:913	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	1	57	theme	delivery	376:383	arg1	system					385:390	a drug delivery system	369:390	a drug delivery system based on bacterial cellulose (BC-Ag-pABA)	369:432	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	2	58	theme	high	496:499	arg1	spectrometry					517:528	high resolution mass spectrometry	496:528	high resolution mass spectrometry	496:528	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	59	theme	in	1498:1499	arg1	effects					1517:1523	the in vivo biological effects	1494:1523	the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer	1494:1691	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	4	60	theme	complex	854:860	arg1	activity					838:845	The in vitro antibacterial activity	811:845	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays	811:913	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	7	61	theme	release	1368:1374	arg1	capacity					1346:1353	an effective capacity	1333:1353	an effective capacity of sustained release of Ag-pABA	1333:1385	The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA.					
32920435	5	62	theme	antiproliferative	1065:1081	arg1	activities					1083:1092	The antiproliferative activities	1061:1092	The antiproliferative activities against a panel of eight tumor cells	1061:1129	The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI).					
32920435	8	63	theme	soft	1611:1614	arg1	tissues					1616:1622	skin and soft tissues	1602:1622	tissues	1616:1622	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	8	64	theme	further	1418:1424	arg1	studies					1426:1432	further studies	1418:1432	further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer	1418:1691	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	3	65	theme	nitrogen	796:803	arg1	atom					805:808	the nitrogen atom	792:808	the nitrogen atom	792:808	The coordination of pABA to the silver ion occurred by the nitrogen atom.					
32920435	4	66	dep	Gram-positive	977:989	arg1	Gram-negative					992:1004	Gram-negative	992:1004	Gram-negative	992:1004	The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus.					
32920435	0	67	theme	sustained	145:153	arg1	release					155:161	sustained release	145:161	sustained release using bacterial cellulose membranes as support	145:208	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	8	68	theme	urinary	1657:1663	arg1	infections					1671:1680	urinary tract infections	1657:1680	urinary tract infections	1657:1680	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	0	69	from	Studies	125:131	arg1	vitro					136:140	vitro	136:140	vitro of sustained release using bacterial cellulose membranes as support	136:208	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	2	70	theme	elemental	476:484	arg1	analysis					486:493	elemental analysis	476:493	elemental analysis	476:493	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	1	71	theme	bacterial	401:409	arg1	BC-Ag-pABA					422:431	BC-Ag-pABA	422:431	BC-Ag-pABA	422:431	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	1	71	theme	bacterial	401:409	arg1	cellulose					411:419	bacterial cellulose	401:419	bacterial cellulose (BC-Ag-pABA)	401:432	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	0	72	dep	activities	14:23	arg1	Studies					125:131	Studies	125:131	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.	0:209	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	2	73	theme	[Ag	715:717	arg1	C7H7NO2					720:726	the coordination formula [Ag (C7H7NO2)2	690:728	the coordination formula [Ag (C7H7NO2)2(NO3)]	690:734	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	2	73	theme	[Ag	715:717	arg1	NO3					730:732	NO3	730:732	NO3	730:732	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	74	theme	observed	1392:1399	arg1	results					1401:1407	The observed results	1388:1407	The observed results	1388:1407	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	8	75	theme	bacterial	1636:1644	arg1	pathogens					1646:1654	bacterial pathogens	1636:1654	bacterial pathogens	1636:1654	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	0	76	theme	cells	67:71	arg1	panel					73:77	a tumor cells panel	59:77	a tumor cells panel of a silver complex with 4-aminobenzoic acid	59:122	Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support.					
32920435	1	77	theme	metal	316:320	arg1	complex					322:328	the metal complex	312:328	the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA)	312:432	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
32920435	7	78	theme	effective	1336:1344	arg1	capacity					1346:1353	an effective capacity	1333:1353	an effective capacity of sustained release of Ag-pABA	1333:1385	The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA.					
32920435	2	79	theme	formula	707:713	arg1	C7H7NO2					720:726	the coordination formula [Ag (C7H7NO2)2	690:728	the coordination formula [Ag (C7H7NO2)2(NO3)]	690:734	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	2	79	theme	formula	707:713	arg1	NO3					730:732	NO3	730:732	NO3	730:732	The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)].					
32920435	8	80	theme	tissues	1616:1622	arg1	treatment					1589:1597	treatment	1589:1597	treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer	1589:1691	The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer.					
32920435	1	81	theme	work	228:231	arg1	aims					215:218	The aims	211:218	The aims of this work	211:231	The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA).					
34766498	7	0	theme	SPIONs	1203:1208	arg1	combination					1177:1187	The combination	1173:1187	The combination of Pt NPs and SPIONs	1173:1208	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	4	1	with	tuned	873:877	arg1	precision					889:897	high precision	884:897	high precision	884:897	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	0	2	theme	Nanoparticle	82:93	arg1	Functionalization					95:111	Localized Nanoparticle Functionalization	72:111	Localized Nanoparticle Functionalization	72:111	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	3	3	theme	hollow	712:717	arg1	spheres					719:725	core-shell or hollow spheres	698:725	core-shell or hollow spheres	698:725	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	4	4	theme	high	884:887	arg1	precision					889:897	high precision	884:897	high precision	884:897	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	2	5	theme	techniques	354:363	arg1	palette					343:349	the palette	339:349	the palette of techniques	339:363	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	7	6	theme	NPs	1195:1197	arg1	combination					1177:1187	The combination	1173:1187	The combination of Pt NPs and SPIONs	1173:1208	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	3	7	theme	magnetic	529:536	arg1	NPs					553:555	NPs	553:555	NPs	553:555	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	7	theme	magnetic	529:536	arg1	nanoparticles					538:550	localized magnetic nanoparticles	519:550	localized magnetic nanoparticles (NPs)	519:556	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	4	8	theme	superparamagnetic	779:795	arg1	NPs					808:810	superparamagnetic iron oxide NPs	779:810	superparamagnetic iron oxide NPs (SPIONs)	779:819	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	4	8	theme	superparamagnetic	779:795	arg1	SPIONs					813:818	SPIONs	813:818	SPIONs	813:818	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	7	9	theme	Pt	1192:1193	arg1	NPs					1195:1197	Pt NPs	1192:1197	Pt NPs	1192:1197	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	1	10	from	fields	167:172	arg1	relevant					147:154	relevant	147:154	relevant	147:154	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	4	11	theme	oxide	802:806	arg1	NPs					808:810	superparamagnetic iron oxide NPs	779:810	superparamagnetic iron oxide NPs (SPIONs)	779:819	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	4	11	theme	oxide	802:806	arg1	SPIONs					813:818	SPIONs	813:818	SPIONs	813:818	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	1	12	theme	simple	289:294	arg1	steps					308:312	simple fabrication steps	289:312	simple fabrication steps	289:312	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	6	13	theme	specific	1126:1133	arg1	locations					1135:1143	specific locations	1126:1143	specific locations	1126:1143	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	3	14	with	spheres	500:506	arg1	NPs					553:555	NPs	553:555	NPs	553:555	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	14	with	spheres	500:506	arg1	nanoparticles					538:550	localized magnetic nanoparticles	519:550	localized magnetic nanoparticles (NPs)	519:556	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	8	15	from	movement	1373:1380	arg1	comparison					1405:1414	comparison	1405:1414	comparison to the controls	1405:1430	Janus Pt/SPIONs increased by five times the directionality and movement of these structures in comparison to the controls.					
34766498	2	16	dep	soft	375:378	arg1	actuable					381:388	actuable	381:388	actuable	381:388	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	6	17	theme	other	1101:1105	arg1	Au					1112:1113	Au	1112:1113	Au	1112:1113	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	6	17	theme	other	1101:1105	arg1	NPs					1107:1109	other NPs	1101:1109	other NPs (Au and Pt)	1101:1121	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	6	17	theme	other	1101:1105	arg1	Pt					1119:1120	Pt	1119:1120	Pt	1119:1120	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	2	18	theme	cellulose	469:477	arg1	process					448:454	the biosynthesis process	431:454	the biosynthesis process of bacterial cellulose	431:477	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	2	19	theme	spherical	390:398	arg1	structures					400:409	soft, actuable spherical structures	375:409	soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose	375:477	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	2	20	theme	bacterial	459:467	arg1	cellulose					469:477	bacterial cellulose	459:477	bacterial cellulose	459:477	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	8	21	dep	directionality	1354:1367	arg1	the					1350:1352	the	1350:1352	the	1350:1352	Janus Pt/SPIONs increased by five times the directionality and movement of these structures in comparison to the controls.					
34766498	7	22	theme	moving	1219:1224	arg1	structures					1236:1245	moving composite structures	1219:1245	moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction	1219:1307	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	6	23	theme	Janus	1155:1159	arg1	structures					1161:1170	Janus structures	1155:1170	Janus structures	1155:1170	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	7	24	theme	magnetic	1264:1271	arg1	field					1273:1277	a magnetic field	1262:1277	a magnetic field	1262:1277	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	0	25	theme	One-Step	0:7	arg1	Biosynthesis					9:20	One-Step Biosynthesis	0:20	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.	0:112	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	1	26	theme	fabrication	296:306	arg1	steps					308:312	simple fabrication steps	289:312	simple fabrication steps	289:312	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	1	27	theme	Actuated	114:121	arg1	structures					123:132	Actuated structures	114:132	Actuated structures	114:132	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	1	28	theme	flexible/soft-base	198:215	arg1	materials					217:225	flexible/soft-base materials	198:225	flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps	198:312	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	2	29	theme	process	448:454	arg1	advantage					418:426	advantage	418:426	advantage of the biosynthesis process of bacterial cellulose	418:477	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	1	30	from	relevant	147:154	arg1	fields					167:172	medical fields	159:172	medical fields	159:172	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	4	31	theme	iron	797:800	arg1	NPs					808:810	superparamagnetic iron oxide NPs	779:810	superparamagnetic iron oxide NPs (SPIONs)	779:819	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	4	31	theme	iron	797:800	arg1	SPIONs					813:818	SPIONs	813:818	SPIONs	813:818	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	3	32	theme	Bacterial	480:488	arg1	BCS					509:511	BCS	509:511	BCS	509:511	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	32	theme	Bacterial	480:488	arg1	spheres					500:506	Bacterial cellulose spheres	480:506	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs)	480:556	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	2	33	theme	biosynthesis	435:446	arg1	process					448:454	the biosynthesis process	431:454	the biosynthesis process of bacterial cellulose	431:477	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	7	34	dep	field	1273:1277	arg1	both					1254:1257	both	1254:1257	both	1254:1257	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	3	35	theme	static	672:677	arg1	culture					679:685	hydrophobic surface-supported static culture	642:685	hydrophobic surface-supported static culture	642:685	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	36	theme	localized	519:527	arg1	NPs					553:555	NPs	553:555	NPs	553:555	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	36	theme	localized	519:527	arg1	nanoparticles					538:550	localized magnetic nanoparticles	519:550	localized magnetic nanoparticles (NPs)	519:556	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	37	theme	cellulose	490:498	arg1	BCS					509:511	BCS	509:511	BCS	509:511	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	37	theme	cellulose	490:498	arg1	spheres					500:506	Bacterial cellulose spheres	480:506	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs)	480:556	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	0	38	theme	Bacterial	39:47	arg1	Spheres					59:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	8	39	theme	structures	1391:1400	arg1	movement					1373:1380	movement	1373:1380	movement	1373:1380	Janus Pt/SPIONs increased by five times the directionality and movement of these structures in comparison to the controls.					
34766498	8	39	theme	structures	1391:1400	arg1	directionality					1354:1367	directionality	1354:1367	directionality	1354:1367	Janus Pt/SPIONs increased by five times the directionality and movement of these structures in comparison to the controls.					
34766498	6	40	theme	spherical	1073:1081	arg1	structures					1083:1092	actuated spherical structures	1064:1092	actuated spherical structures adding other NPs (Au and Pt)	1064:1121	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	0	41	theme	Magnetic	30:37	arg1	Spheres					59:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	3	42	theme	hydrophobic	642:652	arg1	culture					679:685	hydrophobic surface-supported static culture	642:685	hydrophobic surface-supported static culture	642:685	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	0	43	theme	Soft	25:28	arg1	Spheres					59:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	7	44	theme	H2O2	1285:1288	arg1	reaction					1300:1307	a H2O2 oxidation reaction	1283:1307	a H2O2 oxidation reaction	1283:1307	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	0	45	theme	Spheres	59:65	arg1	Biosynthesis					9:20	One-Step Biosynthesis	0:20	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.	0:112	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	3	46	theme	surface-supported	654:670	arg1	culture					679:685	hydrophobic surface-supported static culture	642:685	hydrophobic surface-supported static culture	642:685	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	47	theme	different	593:601	arg1	processes					611:619	two different one-pot processes	589:619	two different one-pot processes	589:619	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	4	48	theme	Magnetic	742:749	arg1	actuability					751:761	Magnetic actuability	742:761	Magnetic actuability	742:761	Magnetic actuability is conferred by superparamagnetic iron oxide NPs (SPIONs), and their location within the structure was finely tuned with high precision.					
34766498	0	49	theme	Cellulose	49:57	arg1	Spheres					59:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	Soft Magnetic Bacterial Cellulose Spheres	25:65	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	3	50	theme	core-shell	698:707	arg1	spheres					719:725	core-shell or hollow spheres	698:725	core-shell or hollow spheres	698:725	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	3	51	theme	one-pot	603:609	arg1	processes					611:619	two different one-pot processes	589:619	two different one-pot processes	589:619	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
34766498	0	52	with	Biosynthesis	9:20	arg1	Functionalization					95:111	Localized Nanoparticle Functionalization	72:111	Localized Nanoparticle Functionalization	72:111	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	6	53	dep	NPs	1107:1109	arg1	Au					1112:1113	Au	1112:1113	Au	1112:1113	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	6	53	dep	NPs	1107:1109	arg1	NPs					1107:1109	other NPs	1101:1109	other NPs (Au and Pt)	1101:1121	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	6	53	dep	NPs	1107:1109	arg1	Pt					1119:1120	Pt	1119:1120	Pt	1119:1120	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	5	54	theme	magnetic	937:944	arg1	response					946:953	magnetic response	937:953	magnetic response	937:953	The size, structure, flexibility and magnetic response of the spheres have been characterized.					
34766498	5	55	theme	spheres	962:968	arg1	structure					910:918	structure	910:918	structure	910:918	The size, structure, flexibility and magnetic response of the spheres have been characterized.					
34766498	5	55	theme	spheres	962:968	arg1	size					904:907	size	904:907	size	904:907	The size, structure, flexibility and magnetic response of the spheres have been characterized.					
34766498	5	55	theme	spheres	962:968	arg1	flexibility					921:931	flexibility	921:931	flexibility	921:931	The size, structure, flexibility and magnetic response of the spheres have been characterized.					
34766498	5	55	theme	spheres	962:968	arg1	response					946:953	magnetic response	937:953	magnetic response	937:953	The size, structure, flexibility and magnetic response of the spheres have been characterized.					
34766498	1	56	theme	biological	244:253	arg1	tissues					255:261	biological tissues	244:261	biological tissues	244:261	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	8	57	theme	Janus	1310:1314	arg1	Pt/SPIONs					1316:1324	Janus Pt/SPIONs	1310:1324	Janus Pt/SPIONs	1310:1324	Janus Pt/SPIONs increased by five times the directionality and movement of these structures in comparison to the controls.					
34766498	1	58	theme	medical	159:165	arg1	fields					167:172	medical fields	159:172	medical fields	159:172	Actuated structures are becoming relevant in medical fields; however, they call for flexible/soft-base materials that comply with biological tissues and can be synthesized in simple fabrication steps.					
34766498	6	59	theme	methodology	1031:1041	arg1	versatility					1012:1022	the versatility	1008:1022	the versatility of the methodology	1008:1041	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	0	60	theme	Localized	72:80	arg1	Functionalization					95:111	Localized Nanoparticle Functionalization	72:111	Localized Nanoparticle Functionalization	72:111	One-Step Biosynthesis of Soft Magnetic Bacterial Cellulose Spheres with Localized Nanoparticle Functionalization.					
34766498	8	61	from	directionality	1354:1367	arg1	comparison					1405:1414	comparison	1405:1414	comparison to the controls	1405:1430	Janus Pt/SPIONs increased by five times the directionality and movement of these structures in comparison to the controls.					
34766498	6	62	theme	actuated	1064:1071	arg1	structures					1083:1092	actuated spherical structures	1064:1092	actuated spherical structures adding other NPs (Au and Pt)	1064:1121	In addition, the versatility of the methodology allows us to produce actuated spherical structures adding other NPs (Au and Pt) in specific locations, creating Janus structures.					
34766498	2	63	theme	soft	375:378	arg1	structures					400:409	soft, actuable spherical structures	375:409	soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose	375:477	In this work, we extend the palette of techniques to afford soft, actuable spherical structures taking advantage of the biosynthesis process of bacterial cellulose.					
34766498	7	64	theme	oxidation	1290:1298	arg1	reaction					1300:1307	a H2O2 oxidation reaction	1283:1307	a H2O2 oxidation reaction	1283:1307	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	7	65	theme	composite	1226:1234	arg1	structures					1236:1245	moving composite structures	1219:1245	moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction	1219:1307	The combination of Pt NPs and SPIONs provides moving composite structures driven both by a magnetic field and a H2O2 oxidation reaction.					
34766498	3	66	dep	biosynthesized	568:581	arg1	achieving					688:696	achieving	688:696	achieving	688:696	Bacterial cellulose spheres (BCS) with localized magnetic nanoparticles (NPs) have been biosynthesized using two different one-pot processes: in agitation and on hydrophobic surface-supported static culture, achieving core-shell or hollow spheres, respectively.					
32096353	8	0	theme	biomaterial	1126:1136	arg1	reduction					1113:1121	a significant reduction	1099:1121	a significant reduction of biomaterial	1099:1136	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	4	1	theme	murine	530:535	arg1	cells					552:556	murine pre-osteoblast cells	530:556	murine pre-osteoblast cells	530:556	Cytocompatibility was evaluated by applying PrestoBlue reagent after exposing murine pre-osteoblast cells to extracts of each biomaterial microspheres.					
32096353	5	2	theme	size	708:711	arg1	8 mm					730:733	8 mm	730:733	8 mm	730:733	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	2	theme	size	708:711	arg1	defect					722:727	a critical size calvaria defect	697:727	a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups	697:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	8	3	theme	6-month	1298:1304	arg1	point					1311:1315	the 6-month time point	1294:1315	the 6-month time point compared with the HA group (p < .05)	1294:1352	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	9	4	with	combination	1401:1411	arg1	hydroxyapatite					1426:1439	hydroxyapatite	1426:1439	hydroxyapatite	1426:1439	Both biomaterials were biocompatible, and the combination of zinc with hydroxyapatite was shown to improve bone repair.					
32096353	7	5	from	differences	1027:1037	arg1	activity					1052:1059	metabolic activity	1042:1059	metabolic activity	1042:1059	The results showed cellular viability for both groups compared to the negative control, and no differences in metabolic activity were observed.					
32096353	3	6	theme	in	338:339	arg1	response					357:364	the in vitro and in vivo biological response	321:364	the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA)	321:449	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	5	7	theme	calvaria	713:720	arg1	8 mm					730:733	8 mm	730:733	8 mm	730:733	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	7	theme	calvaria	713:720	arg1	defect					722:727	a critical size calvaria defect	697:727	a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups	697:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	3	8	theme	nanostructured	369:382	arg1	HA					417:418	HA	417:418	HA	417:418	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	3	8	theme	nanostructured	369:382	arg1	alginate-hydroxyapatite					392:414	nanostructured calcium alginate-hydroxyapatite	369:414	nanostructured calcium alginate-hydroxyapatite (HA)	369:419	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	8	9	from	group	1161:1165	arg1	periods					1187:1193	all experimental periods	1170:1193	all experimental periods	1170:1193	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	7	10	theme	cellular	951:958	arg1	viability					960:968	cellular viability	951:968	cellular viability for both groups	951:984	The results showed cellular viability for both groups compared to the negative control, and no differences in metabolic activity were observed.					
32096353	5	11	theme	biomaterial	654:664	arg1	microspheres					666:677	the biomaterial microspheres	650:677	the biomaterial microspheres	650:677	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	3	12	theme	calcium	384:390	arg1	HA					417:418	HA	417:418	HA	417:418	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	3	12	theme	calcium	384:390	arg1	alginate-hydroxyapatite					392:414	nanostructured calcium alginate-hydroxyapatite	369:414	nanostructured calcium alginate-hydroxyapatite (HA)	369:419	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	6	13	theme	Tissue	816:821	arg1	samples					823:829	Tissue samples	816:829	Tissue samples	816:829	Tissue samples were evaluated through histological and histomorphometric analyses after 1, 3, and 6 months (n = 5).					
32096353	8	14	theme	time	1306:1309	arg1	point					1311:1315	the 6-month time point	1294:1315	the 6-month time point compared with the HA group (p < .05)	1294:1352	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	8	15	theme	significant	1101:1111	arg1	reduction					1113:1121	a significant reduction	1099:1121	a significant reduction of biomaterial	1099:1136	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	3	16	dep	in	325:326	arg1	vitro					328:332	vitro	328:332	vitro	328:332	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	4	17	theme	PrestoBlue	496:505	arg1	reagent					507:513	PrestoBlue reagent	496:513	PrestoBlue reagent	496:513	Cytocompatibility was evaluated by applying PrestoBlue reagent after exposing murine pre-osteoblast cells to extracts of each biomaterial microspheres.					
32096353	8	18	theme	considerable	1207:1218	arg1	amount					1220:1225	a considerable amount	1205:1225	a considerable amount of new bone formation	1205:1247	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	8	18	theme	considerable	1207:1218	arg1	formation					1239:1247	new bone formation	1230:1247	new bone formation	1230:1247	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	3	19	theme	zinc-containing	425:439	arg1	ZnHA					445:448	ZnHA	445:448	ZnHA	445:448	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	3	19	theme	zinc-containing	425:439	arg1	HA					441:442	zinc-containing HA	425:442	zinc-containing HA (ZnHA)	425:449	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	8	20	theme	ZnHA	1156:1159	arg1	group					1161:1165	the ZnHA group	1152:1165	the ZnHA group in all experimental periods	1152:1193	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	5	21	theme	chemical	623:630	arg1	characterization					632:647	physical and chemical characterization	610:647	physical and chemical characterization	610:647	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	8	22	theme	HA	1335:1336	arg1	p < .05					1345:1351	p < .05	1345:1351	p < .05	1345:1351	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	8	22	theme	HA	1335:1336	arg1	group					1338:1342	the HA group	1331:1342	the HA group (p < .05)	1331:1352	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	0	23	theme	Biological	0:9	arg1	evaluation					11:20	Biological evaluation	0:20	Biological evaluation of zinc-containing calcium	0:47	Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration.					
32096353	5	24	theme	critical	699:706	arg1	8 mm					730:733	8 mm	730:733	8 mm	730:733	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	24	theme	critical	699:706	arg1	defect					722:727	a critical size calvaria defect	697:727	a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups	697:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	0	25	theme	zinc-containing	25:39	arg1	calcium					41:47	zinc-containing calcium	25:47	zinc-containing calcium	25:47	Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration.					
32096353	4	26	theme	microspheres	590:601	arg1	extracts					561:568	extracts	561:568	extracts of each biomaterial microspheres	561:601	Cytocompatibility was evaluated by applying PrestoBlue reagent after exposing murine pre-osteoblast cells to extracts of each biomaterial microspheres.					
32096353	7	27	theme	metabolic	1042:1050	arg1	activity					1052:1059	metabolic activity	1042:1059	metabolic activity	1042:1059	The results showed cellular viability for both groups compared to the negative control, and no differences in metabolic activity were observed.					
32096353	8	28	theme	experimental	1174:1185	arg1	periods					1187:1193	all experimental periods	1170:1193	all experimental periods	1170:1193	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	8	29	theme	bone	1234:1237	arg1	formation					1239:1247	new bone formation	1230:1247	new bone formation	1230:1247	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	3	30	dep	in	338:339	arg1	vivo					341:344	vivo	341:344	vivo	341:344	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	1	31	theme	important	130:138	arg1	element					140:146	an important element	127:146	an important element for bone structure and metabolism	127:180	Zinc is an important element for bone structure and metabolism.					
32096353	1	31	theme	important	130:138	arg1	Zinc					119:122	Zinc	119:122	Zinc	119:122	Zinc is an important element for bone structure and metabolism.					
32096353	8	32	theme	new	1230:1232	arg1	formation					1239:1247	new bone formation	1230:1247	new bone formation	1230:1247	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	5	33	theme	HA	796:797	arg1	groups					808:813	the HA and ZnHA groups	792:813	groups	808:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	2	34	theme	repair	269:274	arg1	improvement					249:259	the improvement	245:259	the improvement of bone repair	245:274	Its interaction with hydroxyapatite has been investigated for the improvement of bone repair.					
32096353	0	35	theme	calcium	41:47	arg1	evaluation					11:20	Biological evaluation	0:20	Biological evaluation of zinc-containing calcium	0:47	Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration.					
32096353	5	36	theme	=	754:754	arg1	n					752:752	n = 30	752:757	n = 30	752:757	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	36	theme	=	754:754	arg1	rats					746:749	Wistar rats	739:749	Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups	739:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	3	37	theme	study	299:303	arg1	objective					281:289	The objective	277:289	The objective of this study	277:303	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	2	38	theme	bone	264:267	arg1	repair					269:274	bone repair	264:274	bone repair	264:274	Its interaction with hydroxyapatite has been investigated for the improvement of bone repair.					
32096353	8	39	theme	formation	1239:1247	arg1	amount					1220:1225	a considerable amount	1205:1225	a considerable amount of new bone formation	1205:1247	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	8	39	theme	formation	1239:1247	arg1	formation					1239:1247	new bone formation	1230:1247	new bone formation	1230:1247	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	5	40	theme	ZnHA	803:806	arg1	groups					808:813	the HA and ZnHA groups	792:813	groups	808:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	41	from	defect	722:727	arg1	n					752:752	n = 30	752:757	n = 30	752:757	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	41	from	defect	722:727	arg1	rats					746:749	Wistar rats	739:749	Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups	739:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	3	42	theme	in	325:326	arg1	response					357:364	the in vitro and in vivo biological response	321:364	the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA)	321:449	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	6	43	theme	histomorphometric	871:887	arg1	analyses					889:896	histological and histomorphometric analyses	854:896	histological and histomorphometric analyses	854:896	Tissue samples were evaluated through histological and histomorphometric analyses after 1, 3, and 6 months (n = 5).					
32096353	7	44	theme	negative	1002:1009	arg1	control					1011:1017	the negative control	998:1017	the negative control	998:1017	The results showed cellular viability for both groups compared to the negative control, and no differences in metabolic activity were observed.					
32096353	0	45	theme	composite	73:81	arg1	microspheres					83:94	composite microspheres	73:94	composite microspheres for bone regeneration	73:116	Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration.					
32096353	4	46	theme	biomaterial	578:588	arg1	microspheres					590:601	each biomaterial microspheres	573:601	each biomaterial microspheres	573:601	Cytocompatibility was evaluated by applying PrestoBlue reagent after exposing murine pre-osteoblast cells to extracts of each biomaterial microspheres.					
32096353	8	47	theme	HA	1080:1081	arg1	group					1083:1087	The HA group	1076:1087	The HA group	1076:1087	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	2	48	with	interaction	187:197	arg1	hydroxyapatite					204:217	hydroxyapatite	204:217	hydroxyapatite	204:217	Its interaction with hydroxyapatite has been investigated for the improvement of bone repair.					
32096353	6	49	theme	=	926:926	arg1	months					916:921	1, 3, and 6 months	904:921	1, 3, and 6 months (n = 5)	904:929	Tissue samples were evaluated through histological and histomorphometric analyses after 1, 3, and 6 months (n = 5).					
32096353	6	49	theme	=	926:926	arg1	n					924:924	n = 5	924:928	n = 5	924:928	Tissue samples were evaluated through histological and histomorphometric analyses after 1, 3, and 6 months (n = 5).					
32096353	6	50	theme	histological	854:865	arg1	analyses					889:896	histological and histomorphometric analyses	854:896	histological and histomorphometric analyses	854:896	Tissue samples were evaluated through histological and histomorphometric analyses after 1, 3, and 6 months (n = 5).					
32096353	5	51	theme	physical	610:617	arg1	characterization					632:647	physical and chemical characterization	610:647	physical and chemical characterization	610:647	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	1	52	theme	bone	152:155	arg1	structure					157:165	bone structure	152:165	bone structure	152:165	Zinc is an important element for bone structure and metabolism.					
32096353	5	53	theme	Wistar	739:744	arg1	n					752:752	n = 30	752:757	n = 30	752:757	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	5	53	theme	Wistar	739:744	arg1	rats					746:749	Wistar rats	739:749	Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups	739:813	After physical and chemical characterization, the biomaterial microspheres were implanted in a critical size calvaria defect (8 mm) in Wistar rats (n = 30) that were randomly divided into the HA and ZnHA groups.					
32096353	9	54	theme	bone	1462:1465	arg1	repair					1467:1472	bone repair	1462:1472	bone repair	1462:1472	Both biomaterials were biocompatible, and the combination of zinc with hydroxyapatite was shown to improve bone repair.					
32096353	7	55	dep	showed	944:949	arg1	compared					986:993	compared	986:993	showed cellular viability for both groups compared to the negative control	944:1017	The results showed cellular viability for both groups compared to the negative control, and no differences in metabolic activity were observed.					
32096353	8	56	theme	ZnHA	1278:1281	arg1	spheres					1283:1289	the ZnHA spheres	1274:1289	the ZnHA spheres	1274:1289	The HA group presented a significant reduction of biomaterial compared with the ZnHA group in all experimental periods; however, a considerable amount of new bone formation was observed surrounding the ZnHA spheres at the 6-month time point compared with the HA group (p < .05).					
32096353	0	57	theme	bone	100:103	arg1	regeneration					105:116	bone regeneration	100:116	bone regeneration	100:116	Biological evaluation of zinc-containing calcium alginate-hydroxyapatite composite microspheres for bone regeneration.					
32096353	9	58	theme	zinc	1416:1419	arg1	combination					1401:1411	the combination	1397:1411	the combination of zinc with hydroxyapatite	1397:1439	Both biomaterials were biocompatible, and the combination of zinc with hydroxyapatite was shown to improve bone repair.					
32096353	3	59	theme	biological	346:355	arg1	response					357:364	the in vitro and in vivo biological response	321:364	the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA)	321:449	The objective of this study was to evaluate the in vitro and in vivo biological response to nanostructured calcium alginate-hydroxyapatite (HA) and zinc-containing HA (ZnHA).					
32096353	4	60	theme	pre-osteoblast	537:550	arg1	cells					552:556	murine pre-osteoblast cells	530:556	murine pre-osteoblast cells	530:556	Cytocompatibility was evaluated by applying PrestoBlue reagent after exposing murine pre-osteoblast cells to extracts of each biomaterial microspheres.					
33317218	6	0	dep	way	1114:1116	arg1	deposition					1119:1128	deposition	1119:1128	deposition of ex-situ-prepared AgNP	1119:1153	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	8	1	theme	BP/AgP	1480:1485	arg1	nanocomposite					1487:1499	the BP/AgP nanocomposite	1476:1499	the BP/AgP nanocomposite	1476:1499	Finally, the antibacterial activity of the BP/AgP nanocomposite was tested using selected bacteria biofilms.					
33317218	10	2	from	influence	1752:1760	arg1	body					1778:1781	the bacteria body	1765:1781	the bacteria body	1765:1781	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	5	3	with	AgNP	865:868	arg1	structure					896:904	disturbed electronic structure	875:904	disturbed electronic structure	875:904	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	5	3	with	AgNP	865:868	arg1	properties					920:929	plasmonic properties	910:929	plasmonic properties	910:929	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	7	4	theme	scanning	1286:1293	arg1	calorimetry					1295:1305	differential scanning calorimetry	1273:1305	differential scanning calorimetry	1273:1305	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	3	5	theme	X-ray	536:540	arg1	XPS					570:572	XPS	570:572	XPS	570:572	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	3	5	theme	X-ray	536:540	arg1	spectroscopy					556:567	The X-ray photoelectron spectroscopy	532:567	The X-ray photoelectron spectroscopy (XPS)	532:573	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	6	6	theme	typical	1106:1112	arg1	way					1114:1116	the typical way	1102:1116	the typical way (deposition of ex-situ-prepared AgNP)	1102:1154	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	10	7	from	body	1778:1781	arg1	investigation					1726:1738	the investigation	1722:1738	the investigation of the AgNP influence on the bacteria body	1722:1781	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	2	8	theme	electron	470:477	arg1	SEM					491:493	SEM	491:493	SEM	491:493	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	8	theme	electron	470:477	arg1	microscopy					479:488	scanning electron microscopy	461:488	scanning electron microscopy (SEM)	461:494	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	6	9	theme	synthesis	1181:1189	arg1	approach					1191:1198	the proposed in-situ synthesis approach	1160:1198	the proposed in-situ synthesis approach	1160:1198	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	1	10	theme	in	165:166	arg1	reduction					173:181	in situ reduction	165:181	in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+)	165:228	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	9	11	theme	clear	1574:1578	arg1	properties					1591:1600	clear inhibition properties	1574:1600	clear inhibition properties	1574:1600	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	6	12	theme	proposed	1164:1171	arg1	approach					1191:1198	the proposed in-situ synthesis approach	1160:1198	the proposed in-situ synthesis approach	1160:1198	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	10	13	theme	biocidal	1684:1691	arg1	activity					1693:1700	biocidal activity	1684:1700	biocidal activity	1684:1700	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	7	14	theme	improved	1221:1228	arg1	stability					1238:1246	The improved thermal stability	1217:1246	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP	1217:1335	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	2	15	theme	transmission	421:432	arg1	TEM					455:457	TEM	455:457	TEM	455:457	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	15	theme	transmission	421:432	arg1	microscopy					443:452	transmission electron microscopy	421:452	transmission electron microscopy (TEM)	421:458	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	4	16	theme	metallic	731:738	arg1	nanoparticles					740:752	ultra-small metallic nanoparticles	719:752	ultra-small metallic nanoparticles	719:752	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	3	17	theme	elemental	626:634	arg1	composition					636:646	the elemental composition	622:646	the elemental composition	622:646	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	6	18	theme	AgNP	1150:1153	arg1	deposition					1119:1128	deposition	1119:1128	deposition of ex-situ-prepared AgNP	1119:1153	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	5	19	theme	electronic	885:894	arg1	structure					896:904	disturbed electronic structure	875:904	disturbed electronic structure	875:904	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	11	20	theme	membrane	1871:1878	arg1	properties					1880:1889	the biopolymer membrane properties	1856:1889	the biopolymer membrane properties	1856:1889	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	3	21	theme	chemical	668:675	arg1	state					677:681	chemical state	668:681	chemical state	668:681	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	9	22	theme	multiple	1621:1628	arg1	damages					1630:1636	multiple damages	1621:1636	multiple damages	1621:1636	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	11	23	theme	membrane	2026:2033	arg1	system					2035:2040	a biostatic membrane system	2014:2040	a biostatic membrane system for filtration and separation issues	2014:2077	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	1	24	theme	chitosan	119:126	arg1	BP/AgP					140:145	BP/AgP	140:145	BP/AgP	140:145	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	24	theme	chitosan	119:126	arg1	biopolymer					128:137	chitosan biopolymer	119:137	chitosan biopolymer (BP/AgP)	119:146	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	11	25	dep	filtration	2046:2055	arg1	issues					2072:2077	issues	2072:2077	issues	2072:2077	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	3	26	theme	atoms	694:698	arg1	concentration					649:661	concentration	649:661	concentration	649:661	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	3	26	theme	atoms	694:698	arg1	composition					636:646	the elemental composition	622:646	the elemental composition	622:646	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	3	26	theme	atoms	694:698	arg1	state					677:681	chemical state	668:681	chemical state	668:681	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	7	27	theme	silver	1423:1428	arg1	phase					1430:1434	a low-molecular silver phase	1407:1434	a low-molecular silver phase	1407:1434	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	2	28	theme	whole	382:386	arg1	membrane					394:401	the whole solid membrane	378:401	the whole solid membrane	378:401	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	1	29	theme	membrane	252:259	arg1	system					261:266	a biostatic membrane system	240:266	a biostatic membrane system	240:266	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	4	30	theme	silver	784:789	arg1	ions					791:794	silver ions	784:794	silver ions	784:794	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	11	31	theme	ultra-small	1943:1953	arg1	nanoparticles					1958:1970	ultra-small Ag nanoparticles	1943:1970	ultra-small Ag nanoparticles	1943:1970	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	2	32	theme	silver	313:318	arg1	ions					320:323	silver ions	313:323	silver ions	313:323	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	33	theme	homogeneous	350:360	arg1	distribution					362:373	homogeneous distribution	350:373	homogeneous distribution	350:373	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	0	34	theme	Small	0:4	arg1	AgNP					6:9	Small AgNP	0:9	Small AgNP in the Biopolymer Nanocomposite System.	0:49	Small AgNP in the Biopolymer Nanocomposite System.					
33317218	1	35	theme	[Ag	217:219	arg1	NH3					221:223	[Ag(NH3)2]+	217:227	NH3	221:223	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	35	theme	[Ag	217:219	arg1	complex					208:214	the diamminesilver(I) complex	186:214	the diamminesilver(I) complex ([Ag(NH3)2]+)	186:228	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	36	from	nanoparticles	95:107	arg1	BP/AgP					140:145	BP/AgP	140:145	BP/AgP	140:145	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	36	from	nanoparticles	95:107	arg1	biopolymer					128:137	chitosan biopolymer	119:137	chitosan biopolymer (BP/AgP)	119:146	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	2	37	theme	atomic	501:506	arg1	AFM					526:528	AFM	526:528	AFM	526:528	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	37	theme	atomic	501:506	arg1	microscopy					514:523	atomic force microscopy	501:523	atomic force microscopy (AFM)	501:529	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	5	38	theme	nanometallic	1032:1043	arg1	layer					1045:1049	the nanometallic layer	1028:1049	the nanometallic layer	1028:1049	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	7	39	theme	protective	1387:1396	arg1	layer					1398:1402	the protective layer	1383:1402	the protective layer of a low-molecular silver phase	1383:1434	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	1	40	theme	silver	88:93	arg1	AgNP					110:113	AgNP	110:113	AgNP	110:113	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	40	theme	silver	88:93	arg1	nanoparticles					95:107	ultra-small and stable silver nanoparticles	65:107	ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP)	65:146	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	2	41	theme	crystal	332:338	arg1	form					340:343	their crystal form	326:343	their crystal form	326:343	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	9	42	theme	grafted	1550:1556	arg1	membrane					1558:1565	The grafted membrane	1546:1565	The grafted membrane	1546:1565	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	0	43	theme	Biopolymer	18:27	arg1	System					43:48	the Biopolymer Nanocomposite System	14:48	the Biopolymer Nanocomposite System	14:48	Small AgNP in the Biopolymer Nanocomposite System.					
33317218	2	44	from	AgNP	279:282	arg1	membrane					394:401	the whole solid membrane	378:401	the whole solid membrane	378:401	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	4	45	theme	solid	836:840	arg1	membranes					842:850	solid membranes	836:850	solid membranes	836:850	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	10	46	theme	AFM	1802:1804	arg1	measurements					1806:1817	AFM measurements	1802:1817	AFM measurements	1802:1817	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	10	47	theme	AgNP	1747:1750	arg1	influence					1752:1760	the AgNP influence	1743:1760	the AgNP influence on the bacteria body	1743:1781	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	8	48	theme	selected	1518:1525	arg1	biofilms					1536:1543	selected bacteria biofilms	1518:1543	selected bacteria biofilms	1518:1543	Finally, the antibacterial activity of the BP/AgP nanocomposite was tested using selected bacteria biofilms.					
33317218	1	49	theme	diamminesilver	190:203	arg1	NH3					221:223	[Ag(NH3)2]+	217:227	NH3	221:223	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	49	theme	diamminesilver	190:203	arg1	complex					208:214	the diamminesilver(I) complex	186:214	the diamminesilver(I) complex ([Ag(NH3)2]+)	186:228	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	6	50	theme	ex-situ-prepared	1133:1148	arg1	AgNP					1150:1153	ex-situ-prepared AgNP	1133:1153	ex-situ-prepared AgNP	1133:1153	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	11	51	theme	biostatic	2016:2024	arg1	system					2035:2040	a biostatic membrane system	2014:2040	a biostatic membrane system for filtration and separation issues	2014:2077	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	10	52	theme	bacteria	1769:1776	arg1	body					1778:1781	the bacteria body	1765:1781	the bacteria body	1765:1781	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	1	53	theme	complex	208:214	arg1	reduction					173:181	in situ reduction	165:181	in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+)	165:228	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	8	54	theme	nanocomposite	1487:1499	arg1	activity					1464:1471	the antibacterial activity	1446:1471	the antibacterial activity of the BP/AgP nanocomposite	1446:1499	Finally, the antibacterial activity of the BP/AgP nanocomposite was tested using selected bacteria biofilms.					
33317218	2	55	theme	ions	320:323	arg1	form					340:343	their crystal form	326:343	their crystal form	326:343	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	55	theme	ions	320:323	arg1	source					303:308	a stable source	294:308	a stable source of silver ions	294:323	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	55	theme	ions	320:323	arg1	distribution					362:373	homogeneous distribution	350:373	homogeneous distribution	350:373	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	7	56	theme	calorimetry	1295:1305	arg1	analysis					1316:1323	thermogravimetry and differential scanning calorimetry (TG/DSC) analysis	1252:1323	thermogravimetry and differential scanning calorimetry (TG/DSC) analysis	1252:1323	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	3	57	theme	photoelectron	542:554	arg1	XPS					570:572	XPS	570:572	XPS	570:572	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	3	57	theme	photoelectron	542:554	arg1	spectroscopy					556:567	The X-ray photoelectron spectroscopy	532:567	The X-ray photoelectron spectroscopy (XPS)	532:573	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	10	58	theme	possible	1661:1668	arg1	mechanisms					1670:1679	The possible mechanisms	1657:1679	The possible mechanisms of biocidal activity	1657:1700	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	2	59	theme	scanning	461:468	arg1	SEM					491:493	SEM	491:493	SEM	491:493	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	59	theme	scanning	461:468	arg1	microscopy					479:488	scanning electron microscopy	461:488	scanning electron microscopy (SEM)	461:494	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	7	60	theme	differential	1273:1284	arg1	calorimetry					1295:1305	differential scanning calorimetry	1273:1305	differential scanning calorimetry	1273:1305	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	3	61	theme	Auger	579:583	arg1	analysis					585:592	Auger analysis	579:592	Auger analysis	579:592	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	11	62	theme	Ag	1955:1956	arg1	nanoparticles					1958:1970	ultra-small Ag nanoparticles	1943:1970	ultra-small Ag nanoparticles	1943:1970	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	7	63	theme	thermogravimetry	1252:1267	arg1	analysis					1316:1323	thermogravimetry and differential scanning calorimetry (TG/DSC) analysis	1252:1323	thermogravimetry and differential scanning calorimetry (TG/DSC) analysis	1252:1323	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	6	64	theme	in-situ	1173:1179	arg1	approach					1191:1198	the proposed in-situ synthesis approach	1160:1198	the proposed in-situ synthesis approach	1160:1198	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	5	65	theme	plasmonic	910:918	arg1	properties					920:929	plasmonic properties	910:929	plasmonic properties	910:929	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	10	66	theme	activity	1693:1700	arg1	mechanisms					1670:1679	The possible mechanisms	1657:1679	The possible mechanisms of biocidal activity	1657:1700	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	9	67	theme	inhibition	1580:1589	arg1	properties					1591:1600	clear inhibition properties	1574:1600	clear inhibition properties	1574:1600	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	6	68	theme	significant	1070:1080	arg1	differences					1082:1092	the significant differences	1066:1092	the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach	1066:1198	In this work, the significant differences between the typical way (deposition of ex-situ-prepared AgNP) and the proposed in-situ synthesis approach were determined.					
33317218	9	69	theme	cells	1650:1654	arg1	destruction					1605:1615	destruction	1605:1615	destruction	1605:1615	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	9	69	theme	cells	1650:1654	arg1	damages					1630:1636	multiple damages	1621:1636	multiple damages	1621:1636	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	5	70	theme	biopolymer	984:993	arg1	groups					970:975	amine groups	964:975	amine groups of the biopolymer	964:993	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	5	70	theme	biopolymer	984:993	arg1	biopolymer					984:993	the biopolymer	980:993	the biopolymer	980:993	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	2	71	theme	electron	434:441	arg1	TEM					455:457	TEM	455:457	TEM	455:457	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	71	theme	electron	434:441	arg1	microscopy					443:452	transmission electron microscopy	421:452	transmission electron microscopy (TEM)	421:458	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	7	72	theme	thermal	1230:1236	arg1	stability					1238:1246	The improved thermal stability	1217:1246	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP	1217:1335	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	5	73	theme	disturbed	875:883	arg1	structure					896:904	disturbed electronic structure	875:904	disturbed electronic structure	875:904	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	11	74	theme	biopolymer	1860:1869	arg1	properties					1880:1889	the biopolymer membrane properties	1856:1889	the biopolymer membrane properties	1856:1889	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	3	75	theme	surface	686:692	arg1	atoms					694:698	surface atoms	686:698	surface atoms	686:698	The X-ray photoelectron spectroscopy (XPS) and Auger analysis were applied to investigate the elemental composition, concentration, and chemical state of surface atoms.					
33317218	4	76	theme	ultra-small	719:729	arg1	nanoparticles					740:752	ultra-small metallic nanoparticles	719:752	ultra-small metallic nanoparticles	719:752	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	7	77	theme	phase	1430:1434	arg1	layer					1398:1402	the protective layer	1383:1402	the protective layer of a low-molecular silver phase	1383:1434	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	4	78	theme	ions	791:794	arg1	source					774:779	a steady source	765:779	a steady source of silver ions	765:794	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	1	79	dep	NH3	221:223	arg1	2					225:225	2	225:225	2	225:225	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	5	80	gly	homogeneity	1013:1023	arg1	layer					1045:1049	the nanometallic layer	1028:1049	the nanometallic layer	1028:1049	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	7	81	theme	low-molecular	1409:1421	arg1	phase					1430:1434	a low-molecular silver phase	1407:1434	a low-molecular silver phase	1407:1434	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	9	82	theme	bacteria	1641:1648	arg1	cells					1650:1654	bacteria cells	1641:1654	bacteria cells	1641:1654	The grafted membrane showed clear inhibition properties by destruction and multiple damages of bacteria cells.					
33317218	0	83	from	AgNP	6:9	arg1	System					43:48	the Biopolymer Nanocomposite System	14:48	the Biopolymer Nanocomposite System	14:48	Small AgNP in the Biopolymer Nanocomposite System.					
33317218	1	84	theme	biostatic	242:250	arg1	system					261:266	a biostatic membrane system	240:266	a biostatic membrane system	240:266	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	4	85	theme	steady	767:772	arg1	source					774:779	a steady source	765:779	a steady source of silver ions	765:794	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	5	86	theme	amine	964:968	arg1	groups					970:975	amine groups	964:975	amine groups of the biopolymer	964:993	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	5	86	theme	amine	964:968	arg1	biopolymer					984:993	the biopolymer	980:993	the biopolymer	980:993	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	1	87	theme	ultra-small	65:75	arg1	AgNP					110:113	AgNP	110:113	AgNP	110:113	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	87	theme	ultra-small	65:75	arg1	nanoparticles					95:107	ultra-small and stable silver nanoparticles	65:107	ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP)	65:146	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	4	88	theme	membranes	842:850	arg1	properties					822:831	the biostatic properties	808:831	the biostatic properties of solid membranes	808:850	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	2	89	theme	force	508:512	arg1	AFM					526:528	AFM	526:528	AFM	526:528	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	89	theme	force	508:512	arg1	microscopy					514:523	atomic force microscopy	501:523	atomic force microscopy (AFM)	501:529	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	90	theme	small	273:277	arg1	AgNP					279:282	The small AgNP	269:282	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane	269:401	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	2	90	theme	small	273:277	arg1	nm					287:288	3 nm	285:288	3 nm	285:288	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	11	91	with	integration	1926:1936	arg1	nanoparticles					1958:1970	ultra-small Ag nanoparticles	1943:1970	ultra-small Ag nanoparticles	1943:1970	The results obtained concluded that the biopolymer membrane properties were significantly improved by the integration with ultra-small Ag nanoparticles, which added value to its applications as a biostatic membrane system for filtration and separation issues.					
33317218	1	92	theme	stable	81:86	arg1	AgNP					110:113	AgNP	110:113	AgNP	110:113	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	1	92	theme	stable	81:86	arg1	nanoparticles					95:107	ultra-small and stable silver nanoparticles	65:107	ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP)	65:146	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
33317218	2	93	theme	stable	296:301	arg1	source					303:308	a stable source	294:308	a stable source of silver ions	294:323	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	0	94	theme	Nanocomposite	29:41	arg1	System					43:48	the Biopolymer Nanocomposite System	14:48	the Biopolymer Nanocomposite System	14:48	Small AgNP in the Biopolymer Nanocomposite System.					
33317218	5	95	theme	layer	1045:1049	arg1	homogeneity					1013:1023	the homogeneity	1009:1023	the homogeneity of the nanometallic layer	1009:1049	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	7	96	theme	layer	1398:1402	arg1	presence					1371:1378	the presence	1367:1378	the presence of the protective layer of a low-molecular silver phase	1367:1434	The improved thermal stability (by thermogravimetry and differential scanning calorimetry (TG/DSC) analysis) for BP/AgP was observed and explained by the presence of the protective layer of a low-molecular silver phase.					
33317218	4	97	theme	biostatic	812:820	arg1	properties					822:831	the biostatic properties	808:831	the biostatic properties of solid membranes	808:850	It was found that ultra-small metallic nanoparticles might form a steady source of silver ions and enhance the biostatic properties of solid membranes.					
33317218	5	98	theme	Ultra-small	853:863	arg1	AgNP					865:868	Ultra-small AgNP	853:868	Ultra-small AgNP with disturbed electronic structure and plasmonic properties	853:929	Ultra-small AgNP with disturbed electronic structure and plasmonic properties may generate interaction between amine groups of the biopolymer for improving the homogeneity of the nanometallic layer.					
33317218	2	99	theme	solid	388:392	arg1	membrane					394:401	the whole solid membrane	378:401	the whole solid membrane	378:401	The small AgNP (3 nm) as a stable source of silver ions, their crystal form, and homogeneous distribution in the whole solid membrane were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM).					
33317218	8	100	theme	antibacterial	1450:1462	arg1	activity					1464:1471	the antibacterial activity	1446:1471	the antibacterial activity of the BP/AgP nanocomposite	1446:1499	Finally, the antibacterial activity of the BP/AgP nanocomposite was tested using selected bacteria biofilms.					
33317218	10	101	theme	influence	1752:1760	arg1	investigation					1726:1738	the investigation	1722:1738	the investigation of the AgNP influence on the bacteria body	1722:1781	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	8	102	theme	bacteria	1527:1534	arg1	biofilms					1536:1543	selected bacteria biofilms	1518:1543	selected bacteria biofilms	1518:1543	Finally, the antibacterial activity of the BP/AgP nanocomposite was tested using selected bacteria biofilms.					
33317218	10	103	from	investigation	1726:1738	arg1	body					1778:1781	the bacteria body	1765:1781	the bacteria body	1765:1781	The possible mechanisms of biocidal activity were discussed, and the investigation of the AgNP influence on the bacteria body was illustrated by AFM measurements.					
33317218	1	104	dep	in	165:166	arg1	situ					168:171	situ	168:171	situ	168:171	In this work, ultra-small and stable silver nanoparticles (AgNP) on chitosan biopolymer (BP/AgP) were prepared by in situ reduction of the diamminesilver(I) complex ([Ag(NH3)2]+) to create a biostatic membrane system.					
34878302	6	0	dep	17-year-old	1240:1250	arg1	to					1237:1238	to	1237:1238	to	1237:1238	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	5	1	theme	64-year-old	1052:1062	arg1	adults					1064:1069	18- to 64-year-old adults	1045:1069	18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52)	1045:1100	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	2	2	theme	health	577:582	arg1	centers					584:590	25 health centers	574:590	25 health centers of Catalonia	574:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	10	3	theme	serotypes	1728:1736	arg1	increase					1706:1713	The increase	1702:1713	The increase of non-PCV13 serotypes	1702:1736	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	10	4	theme	serotype	1768:1775	arg1	coverage					1777:1784	serotype coverage	1768:1784	serotype coverage in future vaccines and rethink vaccination programs for older adults	1768:1853	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	0	5	theme	PCV13	188:192	arg1	Introduction					172:183	2017 to 2019 Systematic Introduction	148:183	2017 to 2019 Systematic Introduction of PCV13	148:192	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	3	6	dep	period	678:683	arg1	2013					686:689	2013	686:689	2013	686:689	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	3	6	dep	period	678:683	arg1	2015					695:698	2015	695:698	2015	695:698	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	14	7	theme	dynamics	2426:2433	arg1	incorporation					2335:2347	the incorporation	2331:2347	the incorporation of more serotypes in future vaccines	2331:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	14	7	theme	dynamics	2426:2433	arg1	surveillance					2406:2417	a more thorough surveillance	2390:2417	a more thorough surveillance of the dynamics of this microorganism	2390:2455	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	11	8	theme	vaccine	1917:1923	arg1	incorporation					1890:1902	the incorporation	1886:1902	the incorporation of the PCV13 vaccine	1886:1923	IMPORTANCE We found that with the incorporation of the PCV13 vaccine, the numbers of IPD cases caused by serotypes included in this vaccine decreased in all of the age groups.					
34878302	11	9	theme	age	2020:2022	arg1	groups					2024:2029	the age groups	2016:2029	the age groups	2016:2029	IMPORTANCE We found that with the incorporation of the PCV13 vaccine, the numbers of IPD cases caused by serotypes included in this vaccine decreased in all of the age groups.					
34878302	10	10	from	coverage	1777:1784	arg1	programs					1829:1836	rethink vaccination programs	1809:1836	rethink vaccination programs	1809:1836	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	10	10	from	coverage	1777:1784	arg1	vaccines					1796:1803	future vaccines	1789:1803	future vaccines	1789:1803	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	11	11	theme	cases	1945:1949	arg1	numbers					1930:1936	the numbers	1926:1936	the numbers of IPD cases caused by serotypes included in this vaccine	1926:1994	IMPORTANCE We found that with the incorporation of the PCV13 vaccine, the numbers of IPD cases caused by serotypes included in this vaccine decreased in all of the age groups.					
34878302	3	12	theme	vaccination	716:726	arg1	2019					750:753	2019	750:753	2019	750:753	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	3	12	theme	vaccination	716:726	arg1	SVP					736:738	SVP	736:738	SVP	736:738	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	3	12	theme	vaccination	716:726	arg1	period					728:733	systematic vaccination period	705:733	systematic vaccination period (SVP) (2017 to 2019)	705:754	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	10	13	theme	vaccination	1817:1827	arg1	programs					1829:1836	rethink vaccination programs	1809:1836	rethink vaccination programs	1809:1836	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	3	14	dep	2019	750:753	arg1	to					747:748	to	747:748	to	747:748	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	14	15	theme	thorough	2397:2404	arg1	surveillance					2406:2417	a more thorough surveillance	2390:2417	a more thorough surveillance of the dynamics of this microorganism	2390:2455	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	0	16	dep	2019	156:159	arg1	to					153:154	to	153:154	to	153:154	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	1	17	theme	serotypes	257:265	arg1	distribution					241:252	the distribution	237:252	the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD)	237:369	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	7	18	dep	serotypes	1394:1402	arg1	8					1407:1407	8	1407:1407	8	1407:1407	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	7	18	dep	serotypes	1394:1402	arg1	12F					1414:1416	12F	1414:1416	12F	1414:1416	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	7	18	dep	serotypes	1394:1402	arg1	3					1404:1404	3	1404:1404	3	1404:1404	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	5	19	theme	%	931:931	arg1	[CI					953:955	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99)	820:970	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99)	820:970	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	10	20	theme	rethink	1809:1815	arg1	programs					1829:1836	rethink vaccination programs	1809:1836	rethink vaccination programs	1809:1836	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	5	21	theme	interval	944:951	arg1	%					931:931	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95%	820:931	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99)	820:970	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	8	22	theme	complexes	1471:1479	arg1	proportions					1449:1459	the proportions	1445:1459	the proportions of clonal complexes CC156, CC191, and ST306	1445:1503	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	5	23	dep	64-year-old	1052:1062	arg1	to					1049:1050	to	1049:1050	to	1049:1050	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	9	24	theme	PCV13	1623:1627	arg1	serotypes					1629:1637	PCV13 serotypes	1623:1637	PCV13 serotypes	1623:1637	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	1	25	theme	isolates	318:325	arg1	distribution					241:252	the distribution	237:252	the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD)	237:369	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	6	26	theme	emerging	1188:1195	arg1	serotype					1197:1204	the major emerging serotype	1178:1204	the major emerging serotype in all age groups	1178:1222	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	6	26	theme	emerging	1188:1195	arg1	Serotype					1163:1170	Serotype 8	1163:1172	Serotype 8	1163:1172	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	10	27	theme	older	1842:1846	arg1	adults					1848:1853	older adults	1842:1853	older adults	1842:1853	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	1	28	theme	invasive	335:342	arg1	IPD					366:368	IPD	366:368	IPD	366:368	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	1	28	theme	invasive	335:342	arg1	disease					357:363	invasive pneumococcal disease	335:363	invasive pneumococcal disease (IPD)	335:369	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	1	29	theme	systematic	402:411	arg1	introduction					413:424	systematic introduction	402:424	systematic introduction of PCV13	402:433	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	5	30	theme	decrease	834:841	arg1	%					931:931	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95%	820:931	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99)	820:970	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	2	31	theme	ages	524:527	arg1	patients					508:515	patients	508:515	patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia	508:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	14	32	theme	future	2370:2375	arg1	vaccines					2377:2384	future vaccines	2370:2384	future vaccines	2370:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	7	33	theme	main	1304:1307	arg1	serotypes					1309:1317	the main serotypes	1300:1317	the main serotypes in SVP	1300:1324	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	7	33	theme	main	1304:1307	arg1	24F					1331:1333	24F	1331:1333	24F	1331:1333	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	0	34	theme	Streptococcus	36:48	arg1	Isolates					61:68	Streptococcus pneumoniae Isolates	36:68	Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13	36:192	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	5	35	from	incidence	850:858	arg1	children					876:883	children 5 to 17 years	876:897	children 5 to 17 years old (relative risk [RR]	876:921	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	36	theme	old	899:901	arg1	children					876:883	children 5 to 17 years	876:897	children 5 to 17 years old (relative risk [RR]	876:921	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	2	37	theme	Pneumococcal	436:447	arg1	strains					449:455	Pneumococcal strains	436:455	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia	436:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	9	38	theme	systematic	1678:1687	arg1	vaccination					1689:1699	systematic vaccination	1678:1699	systematic vaccination	1678:1699	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	13	39	theme	serotype	2205:2212	arg1	increase					2193:2200	a significant increase	2179:2200	a significant increase of serotype 3 included in the vaccine	2179:2238	Moreover, a significant increase of serotype 3 included in the vaccine has been observed; this event has been reported by other researchers.					
34878302	5	40	theme	IPD	1032:1034	arg1	cases					1036:1040	IPD cases	1032:1040	IPD cases	1032:1040	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	2	41	with	ages	524:527	arg1	IPD					534:536	IPD	534:536	IPD	534:536	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	5	42	theme	cases	867:871	arg1	incidence					850:858	the incidence	846:858	the incidence of IPD cases in children 5 to 17 years old (relative risk [RR]	846:921	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	12	43	theme	unforeseen	2052:2061	arg1	increase					2063:2070	an unforeseen increase	2049:2070	an unforeseen increase of the serotypes not included in this vaccine causing IPD	2049:2128	Still, there was an unforeseen increase of the serotypes not included in this vaccine causing IPD, especially in the >65-year-old group.					
34878302	0	44	theme	Clonal	14:19	arg1	Composition					21:31	Clonal Composition	14:31	Clonal Composition	14:31	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	7	45	theme	old	1295:1297	arg1	5 years					1287:1293	5 years	1287:1293	5 years old	1287:1297	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	7	46	theme	older	1364:1368	arg1	adults					1357:1362	adults	1357:1362	adults older than 65 years	1357:1382	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	5	47	from	increase	1003:1010	arg1	incidence					1019:1027	the incidence	1015:1027	the incidence of IPD cases	1015:1040	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	47	from	increase	1003:1010	arg1	adults					1064:1069	18- to 64-year-old adults	1045:1069	18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52)	1045:1100	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	47	from	increase	1003:1010	arg1	adults					1106:1111	adults	1106:1111	adults	1106:1111	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	1	48	theme	composition	278:288	arg1	distribution					241:252	the distribution	237:252	the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD)	237:369	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	3	49	theme	study	624:628	arg1	periods					630:636	Two study periods	620:636	Two study periods	620:636	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	4	50	theme	isolates	774:781	arg1	total					759:763	A total	757:763	A total of 2,303 isolates	757:781	A total of 2,303 isolates were analyzed.					
34878302	1	51	theme	Streptococcus	293:305	arg1	isolates					318:325	Streptococcus pneumoniae isolates	293:325	Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD)	293:369	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	5	52	dep	RR	1132:1133	arg1	CI					1145:1146	95% CI 1.09 to 1.38	1141:1159	95% CI 1.09 to 1.38	1141:1159	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	10	53	theme	non-PCV13	1718:1726	arg1	serotypes					1728:1736	non-PCV13 serotypes	1718:1736	non-PCV13 serotypes	1718:1736	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	3	54	theme	vaccination	666:676	arg1	period					678:683	presystematic vaccination period	652:683	presystematic vaccination period (2013 and 2015)	652:699	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	8	55	from	decrease	1433:1440	arg1	proportions					1449:1459	the proportions	1445:1459	the proportions of clonal complexes CC156, CC191, and ST306	1445:1503	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	8	55	from	decrease	1433:1440	arg1	those					1524:1528	those	1524:1528	those	1524:1528	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	6	56	theme	17-year-old	1240:1250	arg1	children					1252:1259	5- to 17-year-old children	1234:1259	5- to 17-year-old children	1234:1259	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	5	57	theme	relative	904:911	arg1	[RR					918:920	relative risk [RR	904:920	relative risk [RR	904:920	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	58	dep	old	1127:1129	arg1	RR					1132:1133	RR	1132:1133	RR	1132:1133	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	59	dep	children	876:883	arg1	to					887:888	to	887:888	to	887:888	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	59	dep	children	876:883	arg1	17 years					890:897	17 years	890:897	children 5 to 17 years old (relative risk [RR]	876:921	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	14	60	from	serotypes	2357:2365	arg1	vaccines					2377:2384	future vaccines	2370:2384	future vaccines	2370:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	9	61	dep	importance	1653:1662	arg1	the					1649:1651	the	1649:1651	the	1649:1651	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	1	62	theme	study	212:216	arg1	goal					199:202	The goal	195:202	The goal of this study	195:216	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	13	63	theme	significant	2181:2191	arg1	increase					2193:2200	a significant increase	2179:2200	a significant increase of serotype 3 included in the vaccine	2179:2238	Moreover, a significant increase of serotype 3 included in the vaccine has been observed; this event has been reported by other researchers.					
34878302	0	64	theme	Systematic	161:170	arg1	Introduction					172:183	2017 to 2019 Systematic Introduction	148:183	2017 to 2019 Systematic Introduction of PCV13	148:192	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	5	65	dep	[CI	953:955	arg1	to					963:964	to	963:964	to	963:964	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	1	66	theme	PCV13	429:433	arg1	introduction					413:424	systematic introduction	402:424	systematic introduction of PCV13	402:433	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	6	67	theme	age	1213:1215	arg1	groups					1217:1222	all age groups	1209:1222	all age groups	1209:1222	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	5	68	dep	1.52	1096:1099	arg1	to					1093:1094	to	1093:1094	to	1093:1094	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	14	69	theme	microorganism	2443:2455	arg1	dynamics					2426:2433	the dynamics	2422:2433	the dynamics of this microorganism	2422:2455	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	3	70	theme	systematic	705:714	arg1	2019					750:753	2019	750:753	2019	750:753	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	3	70	theme	systematic	705:714	arg1	SVP					736:738	SVP	736:738	SVP	736:738	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	3	70	theme	systematic	705:714	arg1	period					728:733	systematic vaccination period	705:733	systematic vaccination period (SVP) (2017 to 2019)	705:754	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	13	71	theme	other	2291:2295	arg1	researchers					2297:2307	other researchers	2291:2307	other researchers	2291:2307	Moreover, a significant increase of serotype 3 included in the vaccine has been observed; this event has been reported by other researchers.					
34878302	11	72	theme	PCV13	1911:1915	arg1	vaccine					1917:1923	the PCV13 vaccine	1907:1923	the PCV13 vaccine	1907:1923	IMPORTANCE We found that with the incorporation of the PCV13 vaccine, the numbers of IPD cases caused by serotypes included in this vaccine decreased in all of the age groups.					
34878302	14	73	from	surveillance	2406:2417	arg1	vaccines					2377:2384	future vaccines	2370:2384	future vaccines	2370:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	5	74	theme	significant	991:1001	arg1	increase					1003:1010	a significant increase	989:1010	a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults	989:1111	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	2	75	theme	Catalonia	595:603	arg1	centers					584:590	25 health centers	574:590	25 health centers of Catalonia	574:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	9	76	theme	steady	1573:1578	arg1	decrease					1580:1587	A steady decrease	1571:1587	A steady decrease in the incidence of IPD caused by PCV13 serotypes	1571:1637	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	10	77	theme	future	1789:1794	arg1	vaccines					1796:1803	future vaccines	1789:1803	future vaccines	1789:1803	The increase of non-PCV13 serotypes highlights the need to expand serotype coverage in future vaccines and rethink vaccination programs for older adults.					
34878302	11	78	theme	IPD	1941:1943	arg1	cases					1945:1949	IPD cases	1941:1949	IPD cases caused by serotypes included in this vaccine	1941:1994	IMPORTANCE We found that with the incorporation of the PCV13 vaccine, the numbers of IPD cases caused by serotypes included in this vaccine decreased in all of the age groups.					
34878302	8	79	theme	significant	1421:1431	arg1	decrease					1433:1440	A significant decrease	1419:1440	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306	1419:1503	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	0	80	from	IPD	78:80	arg1	Adults					98:103	Adults	98:103	Adults	98:103	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	0	80	from	IPD	78:80	arg1	Children					85:92	Children	85:92	Children	85:92	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	0	80	from	IPD	78:80	arg1	Catalonia					108:116	Catalonia	108:116	Catalonia	108:116	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	5	81	dep	adults	1064:1069	arg1	RR					1072:1073	RR	1072:1073	RR	1072:1073	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	9	82	from	decrease	1580:1587	arg1	incidence					1596:1604	the incidence	1592:1604	the incidence of IPD caused by PCV13 serotypes	1592:1637	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	9	83	theme	IPD	1609:1611	arg1	incidence					1596:1604	the incidence	1592:1604	the incidence of IPD caused by PCV13 serotypes	1592:1637	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	5	84	dep	old	899:901	arg1	[RR					918:920	relative risk [RR	904:920	relative risk [RR	904:920	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	7	85	from	serotypes	1394:1402	arg1	adults					1357:1362	adults	1357:1362	adults older than 65 years	1357:1382	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	12	86	theme	serotypes	2079:2087	arg1	increase					2063:2070	an unforeseen increase	2049:2070	an unforeseen increase of the serotypes not included in this vaccine causing IPD	2049:2128	Still, there was an unforeseen increase of the serotypes not included in this vaccine causing IPD, especially in the >65-year-old group.					
34878302	8	87	theme	clonal	1464:1469	arg1	complexes					1471:1479	clonal complexes CC156, CC191, and ST306	1464:1503	clonal complexes CC156, CC191, and ST306	1464:1503	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	14	88	theme	serotypes	2357:2365	arg1	incorporation					2335:2347	the incorporation	2331:2347	the incorporation of more serotypes in future vaccines	2331:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	14	88	theme	serotypes	2357:2365	arg1	surveillance					2406:2417	a more thorough surveillance	2390:2417	a more thorough surveillance of the dynamics of this microorganism	2390:2455	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	8	89	from	increase	1512:1519	arg1	proportions					1449:1459	the proportions	1445:1459	the proportions of clonal complexes CC156, CC191, and ST306	1445:1503	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	8	89	from	increase	1512:1519	arg1	those					1524:1528	those	1524:1528	those	1524:1528	A significant decrease in the proportions of clonal complexes CC156, CC191, and ST306 and an increase in those of CC180, CC53, and CC404 were observed.					
34878302	5	90	theme	significant	822:832	arg1	decrease					834:841	a significant decrease	820:841	a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99)	820:970	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	2	91	theme	sterile	480:486	arg1	sites					488:492	normally sterile sites	471:492	normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia	471:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	6	92	from	serotype	1197:1204	arg1	groups					1217:1222	all age groups	1209:1222	all age groups	1209:1222	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	6	92	from	serotype	1197:1204	arg1	children					1252:1259	5- to 17-year-old children	1234:1259	5- to 17-year-old children	1234:1259	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	1	93	theme	pneumococcal	344:355	arg1	IPD					366:368	IPD	366:368	IPD	366:368	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	1	93	theme	pneumococcal	344:355	arg1	disease					357:363	invasive pneumococcal disease	335:363	invasive pneumococcal disease (IPD)	335:369	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	5	94	from	decrease	834:841	arg1	incidence					850:858	the incidence	846:858	the incidence of IPD cases in children 5 to 17 years old (relative risk [RR]	846:921	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	1	95	dep	Streptococcus	293:305	arg1	pneumoniae					307:316	pneumoniae	307:316	pneumoniae	307:316	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	14	96	from	incorporation	2335:2347	arg1	vaccines					2377:2384	future vaccines	2370:2384	future vaccines	2370:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	2	97	attach	isolated	457:464	arg2	strains					449:455	Pneumococcal strains	436:455	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia	436:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	2	97	attach	isolated	457:464	arg1	sites					488:492	normally sterile sites	471:492	normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia	471:603	Pneumococcal strains isolated from normally sterile sites obtained from patients of all ages with IPD received between 2013 and 2019 from 25 health centers of Catalonia were included.					
34878302	14	98	theme	more	2352:2355	arg1	serotypes					2357:2365	more serotypes	2352:2365	more serotypes in future vaccines	2352:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	5	99	theme	IPD	863:865	arg1	cases					867:871	IPD cases	863:871	IPD cases	863:871	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	11	100	dep	IMPORTANCE	1856:1865	arg1	We					1867:1868	We	1867:1868	We	1867:1868	IMPORTANCE We found that with the incorporation of the PCV13 vaccine, the numbers of IPD cases caused by serotypes included in this vaccine decreased in all of the age groups.					
34878302	9	101	theme	vaccination	1689:1699	arg1	impact					1668:1673	impact	1668:1673	impact	1668:1673	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	9	101	theme	vaccination	1689:1699	arg1	importance					1653:1662	importance	1653:1662	importance	1653:1662	A steady decrease in the incidence of IPD caused by PCV13 serotypes indicates the importance and impact of systematic vaccination.					
34878302	5	102	theme	cases	1036:1040	arg1	incidence					1019:1027	the incidence	1015:1027	the incidence of IPD cases	1015:1040	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	103	theme	old	1127:1129	arg1	65 years					1118:1125	65 years	1118:1125	65 years old (RR 1.23; 95% CI 1.09 to 1.38)	1118:1160	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	6	104	theme	major	1182:1186	arg1	serotype					1197:1204	the major emerging serotype	1178:1204	the major emerging serotype in all age groups	1178:1222	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	6	104	theme	major	1182:1186	arg1	Serotype					1163:1170	Serotype 8	1163:1172	Serotype 8	1163:1172	Serotype 8 was the major emerging serotype in all age groups except in 5- to 17-year-old children.					
34878302	1	105	theme	clonal	271:276	arg1	composition					278:288	clonal composition	271:288	clonal composition	271:288	The goal of this study was to investigate the distribution of serotypes and clonal composition of Streptococcus pneumoniae isolates causing invasive pneumococcal disease (IPD) in Catalonia, before and after systematic introduction of PCV13.					
34878302	7	106	from	serotypes	1309:1317	arg1	SVP					1322:1324	SVP	1322:1324	SVP	1322:1324	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	5	107	dep	CI	1145:1146	arg1	to					1153:1154	to	1153:1154	to	1153:1154	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	0	108	theme	Isolates	61:68	arg1	Serotypes					0:8	Serotypes	0:8	Serotypes	0:8	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	0	108	theme	Isolates	61:68	arg1	Composition					21:31	Clonal Composition	14:31	Clonal Composition	14:31	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	0	109	dep	Streptococcus	36:48	arg1	pneumoniae					50:59	pneumoniae	50:59	pneumoniae	50:59	Serotypes and Clonal Composition of Streptococcus pneumoniae Isolates Causing IPD in Children and Adults in Catalonia before 2013 to 2015 and after 2017 to 2019 Systematic Introduction of PCV13.					
34878302	3	110	theme	presystematic	652:664	arg1	period					678:683	presystematic vaccination period	652:683	presystematic vaccination period (2013 and 2015)	652:699	Two study periods were defined: presystematic vaccination period (2013 and 2015) and systematic vaccination period (SVP) (2017 to 2019).					
34878302	4	111	theme	2,303	768:772	arg1	isolates					774:781	2,303 isolates	768:781	2,303 isolates	768:781	A total of 2,303 isolates were analyzed.					
34878302	5	112	dep	RR	1072:1073	arg1	%					1083:1083	95% CI 1.16 to 1.52	1081:1099	RR 1.33; 95% CI 1.16 to 1.52	1072:1099	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	7	113	from	24F	1331:1333	arg1	children					1265:1272	children	1265:1272	children younger than 5 years old	1265:1297	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	7	114	theme	younger	1274:1280	arg1	children					1265:1272	children	1265:1272	children younger than 5 years old	1265:1297	In children younger than 5 years old, the main serotypes in SVP were 24F, 15A, and 3, while in adults older than 65 years they were serotypes 3, 8, and 12F.					
34878302	12	115	theme	>65-year-old	2149:2160	arg1	group					2162:2166	the >65-year-old group	2145:2166	the >65-year-old group	2145:2166	Still, there was an unforeseen increase of the serotypes not included in this vaccine causing IPD, especially in the >65-year-old group.					
34878302	5	116	theme	%	1143:1143	arg1	CI					1145:1146	95% CI 1.09 to 1.38	1141:1159	95% CI 1.09 to 1.38	1141:1159	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	5	117	theme	risk	913:916	arg1	[RR					918:920	relative risk [RR	904:920	relative risk [RR	904:920	In the SVP, there was a significant decrease in the incidence of IPD cases in children 5 to 17 years old (relative risk [RR] 0.61; 95% confidence interval [CI] 0.38 to 0.99), while there was a significant increase in the incidence of IPD cases in 18- to 64-year-old adults (RR 1.33; 95% CI 1.16 to 1.52) and adults over 65 years old (RR 1.23; 95% CI 1.09 to 1.38).					
34878302	14	118	from	vaccines	2377:2384	arg1	incorporation					2335:2347	the incorporation	2331:2347	the incorporation of more serotypes in future vaccines	2331:2384	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
34878302	14	118	from	vaccines	2377:2384	arg1	surveillance					2406:2417	a more thorough surveillance	2390:2417	a more thorough surveillance of the dynamics of this microorganism	2390:2455	These facts call for the incorporation of more serotypes in future vaccines and a more thorough surveillance of the dynamics of this microorganism.					
32563954	0	0	theme	reliable	73:80	arg1	zonulin					6:12	Serum zonulin	0:12	Serum zonulin measured by enzyme-linked immunosorbent assay	0:58	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	0	0	theme	reliable	73:80	arg1	marker					82:87	a reliable marker	71:87	a reliable marker of small intestinal permeability in healthy adults	71:138	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	10	1	theme	=	1284:1284	arg1	r					1282:1282	r = .437	1282:1289	r = .437	1282:1289	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	5	2	theme	C-reactive	641:650	arg1	protein					652:658	high-sensitivity C-reactive protein	624:658	high-sensitivity C-reactive protein (hs-CRP)	624:667	Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum.					
32563954	5	2	theme	C-reactive	641:650	arg1	hs-CRP					661:666	hs-CRP	661:666	hs-CRP	661:666	Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum.					
32563954	2	3	theme	independent	352:362	arg1	biomarker					364:372	an independent biomarker	349:372	an independent biomarker of IP	349:378	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	2	3	theme	independent	352:362	arg1	zonulin					314:320	zonulin	314:320	zonulin	314:320	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	10	4	theme	moderate	1226:1233	arg1	correlation					1244:1254	a moderate positive correlation	1224:1254	a moderate positive correlation with waist circumference (r = .437, P = .042)	1224:1300	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	11	5	theme	powered	1490:1496	arg1	studies					1498:1504	larger powered studies	1483:1504	larger powered studies that address the limitations of the present study	1483:1554	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	3	6	theme	healthy	497:503	arg1	adults					505:510	healthy adults	497:510	healthy adults	497:510	This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults.					
32563954	7	7	theme	age	868:870	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	7	theme	age	868:870	arg1	males					854:858	12 males	851:858	12 males	851:858	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	7	theme	age	868:870	arg1	kg/m2					901:905	body mass index 24 kg/m2	882:905	body mass index 24 kg/m2	882:905	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	7	theme	age	868:870	arg1	circumference					914:926	waist circumference 77cm	908:931	waist circumference 77cm	908:931	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	4	8	theme	small	570:574	arg1	IP					576:577	small IP	570:577	small IP	570:577	The urinary lactulose-rhamnose ratio was used to measure small IP.					
32563954	8	9	dep	=	1015:1015	arg1	-.016					1017:1021	-.016	1017:1021	-.016	1017:1021	No correlation was observed between the lactulose-rhamnose ratio and zonulin (r = -.016, P = .929).					
32563954	8	10	theme	=	1015:1015	arg1	r					1013:1013	r	1013:1013	r = -.016	1013:1021	No correlation was observed between the lactulose-rhamnose ratio and zonulin (r = -.016, P = .929).					
32563954	11	11	theme	important	1399:1407	arg1	considerations					1409:1422	important considerations	1399:1422	important considerations for the measurement of small IP	1399:1454	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	9	12	dep	displayed	1064:1072	arg1	n					1114:1114	n 20	1114:1117	n 20	1114:1117	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	4	13	used	used	554:557	arg2	ratio					544:548	The urinary lactulose-rhamnose ratio	513:548	The urinary lactulose-rhamnose ratio	513:548	The urinary lactulose-rhamnose ratio was used to measure small IP.					
32563954	9	14	theme	=	1122:1122	arg1	r					1120:1120	r	1120:1120	r = .536	1120:1127	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	10	15	theme	female	1305:1310	arg1	participants					1312:1323	female participants	1305:1323	female participants	1305:1323	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	0	16	theme	intestinal	98:107	arg1	permeability					109:120	small intestinal permeability	92:120	small intestinal permeability	92:120	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	5	17	theme	Serum	580:584	arg1	zonulin					586:592	Serum zonulin	580:592	Serum zonulin	580:592	Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum.					
32563954	6	18	theme	Body	693:696	arg1	composition					698:708	Body composition	693:708	Body composition	693:708	Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected.					
32563954	1	19	theme	intestinal	165:174	arg1	IP					190:191	IP	190:191	IP	190:191	The association between intestinal permeability (IP) and body composition remains unclear.					
32563954	1	19	theme	intestinal	165:174	arg1	permeability					176:187	intestinal permeability	165:187	intestinal permeability (IP)	165:192	The association between intestinal permeability (IP) and body composition remains unclear.					
32563954	0	20	theme	small	92:96	arg1	permeability					109:120	small intestinal permeability	92:120	small intestinal permeability	92:120	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	7	21	theme	waist	908:912	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	21	theme	waist	908:912	arg1	circumference					914:926	waist circumference 77cm	908:931	waist circumference 77cm	908:931	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	0	22	theme	Serum	0:4	arg1	zonulin					6:12	Serum zonulin	0:12	Serum zonulin measured by enzyme-linked immunosorbent assay	0:58	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	0	22	theme	Serum	0:4	arg1	marker					82:87	a reliable marker	71:87	a reliable marker of small intestinal permeability in healthy adults	71:138	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	3	23	theme	small	439:443	arg1	IP					445:446	small IP	439:446	small IP	439:446	This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults.					
32563954	10	24	with	correlation	1244:1254	arg1	circumference					1267:1279	waist circumference	1261:1279	waist circumference (r = .437, P = .042)	1261:1300	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	9	25	theme	composition	1172:1182	arg1	measures					1184:1191	body composition measures	1167:1191	body composition measures	1167:1191	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	9	26	theme	P	1130:1130	arg1	.018					1134:1137	P = .018	1130:1137	P = .018	1130:1137	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	11	27	theme	small	1447:1451	arg1	IP					1453:1454	small IP	1447:1454	small IP	1447:1454	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	3	28	theme	pilot	386:390	arg1	study					392:396	This pilot study	381:396	This pilot study	381:396	This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults.					
32563954	5	29	theme	high-sensitivity	624:639	arg1	protein					652:658	high-sensitivity C-reactive protein	624:658	high-sensitivity C-reactive protein (hs-CRP)	624:667	Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum.					
32563954	5	29	theme	high-sensitivity	624:639	arg1	hs-CRP					661:666	hs-CRP	661:666	hs-CRP	661:666	Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum.					
32563954	2	30	used	used	341:344	arg2	zonulin					314:320	zonulin	314:320	zonulin	314:320	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	2	30	used	used	341:344	arg2	biomarker					364:372	an independent biomarker	349:372	an independent biomarker of IP	349:378	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	9	31	theme	lactulose-rhamnose	1039:1056	arg1	ratio					1058:1062	The lactulose-rhamnose ratio	1035:1062	The lactulose-rhamnose ratio	1035:1062	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	2	32	theme	IP	377:378	arg1	biomarker					364:372	an independent biomarker	349:372	an independent biomarker of IP	349:378	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	2	32	theme	IP	377:378	arg1	zonulin					314:320	zonulin	314:320	zonulin	314:320	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	10	33	theme	waist	1261:1265	arg1	circumference					1267:1279	waist circumference	1261:1279	waist circumference (r = .437, P = .042)	1261:1300	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	9	34	theme	=	1132:1132	arg1	.018					1134:1137	P = .018	1130:1137	P = .018	1130:1137	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	0	35	link	enzyme-linked	26:38	arg1	assay					54:58	enzyme-linked immunosorbent assay	26:58	enzyme-linked immunosorbent assay	26:58	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	3	36	theme	zonulin	449:455	arg1	concentrations					457:470	zonulin concentrations	449:470	zonulin concentrations	449:470	This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults.					
32563954	11	37	theme	larger	1483:1488	arg1	studies					1498:1504	larger powered studies	1483:1504	larger powered studies that address the limitations of the present study	1483:1554	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	0	38	theme	permeability	109:120	arg1	zonulin					6:12	Serum zonulin	0:12	Serum zonulin measured by enzyme-linked immunosorbent assay	0:58	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	0	38	theme	permeability	109:120	arg1	marker					82:87	a reliable marker	71:87	a reliable marker of small intestinal permeability in healthy adults	71:138	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	9	39	dep	n	1114:1114	arg1	r					1120:1120	r	1120:1120	r = .536	1120:1127	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	9	39	dep	n	1114:1114	arg1	.018					1134:1137	P = .018	1130:1137	P = .018	1130:1137	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	0	40	theme	immunosorbent	40:52	arg1	assay					54:58	enzyme-linked immunosorbent assay	26:58	enzyme-linked immunosorbent assay	26:58	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	7	41	theme	median	861:866	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	41	theme	median	861:866	arg1	males					854:858	12 males	851:858	12 males	851:858	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	41	theme	median	861:866	arg1	kg/m2					901:905	body mass index 24 kg/m2	882:905	body mass index 24 kg/m2	882:905	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	41	theme	median	861:866	arg1	circumference					914:926	waist circumference 77cm	908:931	waist circumference 77cm	908:931	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	10	42	dep	=	1294:1294	arg1	r					1282:1282	r = .437	1282:1289	r = .437	1282:1289	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	1	43	theme	body	198:201	arg1	composition					203:213	body composition	198:213	body composition	198:213	The association between intestinal permeability (IP) and body composition remains unclear.					
32563954	0	44	theme	enzyme-linked	26:38	arg1	assay					54:58	enzyme-linked immunosorbent assay	26:58	enzyme-linked immunosorbent assay	26:58	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	4	45	theme	urinary	517:523	arg1	ratio					544:548	The urinary lactulose-rhamnose ratio	513:548	The urinary lactulose-rhamnose ratio	513:548	The urinary lactulose-rhamnose ratio was used to measure small IP.					
32563954	0	46	theme	healthy	125:131	arg1	adults					133:138	healthy adults	125:138	healthy adults	125:138	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	9	47	theme	strong	1076:1081	arg1	correlation					1092:1102	a strong positive correlation	1074:1102	a strong positive correlation	1074:1102	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	10	48	dep	circumference	1267:1279	arg1	=					1294:1294	=	1294:1294	=	1294:1294	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	6	49	theme	anthropometric	766:779	arg1	measurements					781:792	anthropometric measurements	766:792	anthropometric measurements	766:792	Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected.					
32563954	8	50	dep	zonulin	1004:1010	arg1	=					1026:1026	=	1026:1026	=	1026:1026	No correlation was observed between the lactulose-rhamnose ratio and zonulin (r = -.016, P = .929).					
32563954	10	51	dep	=	1349:1349	arg1	r					1337:1337	r	1337:1337	r = .485	1337:1344	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	11	52	theme	study	1550:1554	arg1	limitations					1523:1533	the limitations	1519:1533	the limitations of the present study	1519:1554	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	9	53	theme	positive	1083:1090	arg1	correlation					1092:1102	a strong positive correlation	1074:1102	a strong positive correlation	1074:1102	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	3	54	theme	body	477:480	arg1	composition					482:492	body composition	477:492	body composition	477:492	This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults.					
32563954	11	55	theme	IP	1453:1454	arg1	measurement					1432:1442	the measurement	1428:1442	the measurement of small IP	1428:1454	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	11	56	theme	present	1542:1548	arg1	study					1550:1554	the present study	1538:1554	the present study	1538:1554	These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.					
32563954	7	57	theme	index	892:896	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	57	theme	index	892:896	arg1	kg/m2					901:905	body mass index 24 kg/m2	882:905	body mass index 24 kg/m2	882:905	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	4	58	theme	lactulose-rhamnose	525:542	arg1	ratio					544:548	The urinary lactulose-rhamnose ratio	513:548	The urinary lactulose-rhamnose ratio	513:548	The urinary lactulose-rhamnose ratio was used to measure small IP.					
32563954	8	59	theme	lactulose-rhamnose	975:992	arg1	ratio					994:998	the lactulose-rhamnose ratio	971:998	the lactulose-rhamnose ratio	971:998	No correlation was observed between the lactulose-rhamnose ratio and zonulin (r = -.016, P = .929).					
32563954	2	60	theme	sugar-absorption	263:278	arg1	arduous					288:294	arduous	288:294	arduous	288:294	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	2	60	theme	sugar-absorption	263:278	arg1	test					280:283	The gold standard differential sugar-absorption test	232:283	The gold standard differential sugar-absorption test	232:283	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	6	61	theme	X-ray	741:745	arg1	absorptiometry					747:760	dual-energy X-ray absorptiometry	729:760	dual-energy X-ray absorptiometry	729:760	Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected.					
32563954	10	62	theme	positive	1235:1242	arg1	correlation					1244:1254	a moderate positive correlation	1224:1254	a moderate positive correlation with waist circumference (r = .437, P = .042)	1224:1300	Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants.					
32563954	9	63	theme	body	1167:1170	arg1	measures					1184:1191	body composition measures	1167:1191	body composition measures	1167:1191	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	2	64	theme	differential	250:261	arg1	arduous					288:294	arduous	288:294	arduous	288:294	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	2	64	theme	differential	250:261	arg1	test					280:283	The gold standard differential sugar-absorption test	232:283	The gold standard differential sugar-absorption test	232:283	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	6	65	theme	dual-energy	729:739	arg1	absorptiometry					747:760	dual-energy X-ray absorptiometry	729:760	dual-energy X-ray absorptiometry	729:760	Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected.					
32563954	7	66	theme	body	882:885	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	66	theme	body	882:885	arg1	kg/m2					901:905	body mass index 24 kg/m2	882:905	body mass index 24 kg/m2	882:905	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	8	67	dep	=	1026:1026	arg1	r					1013:1013	r	1013:1013	r = -.016	1013:1021	No correlation was observed between the lactulose-rhamnose ratio and zonulin (r = -.016, P = .929).					
32563954	0	68	from	marker	82:87	arg1	adults					133:138	healthy adults	125:138	healthy adults	125:138	Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults.					
32563954	2	69	theme	standard	241:248	arg1	arduous					288:294	arduous	288:294	arduous	288:294	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	2	69	theme	standard	241:248	arg1	test					280:283	The gold standard differential sugar-absorption test	232:283	The gold standard differential sugar-absorption test	232:283	The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP.					
32563954	3	70	from	association	419:429	arg1	adults					505:510	healthy adults	497:510	healthy adults	497:510	This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults.					
32563954	7	71	theme	mass	887:890	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	71	theme	mass	887:890	arg1	kg/m2					901:905	body mass index 24 kg/m2	882:905	body mass index 24 kg/m2	882:905	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	9	72	dep	=	1122:1122	arg1	.536					1124:1127	.536	1124:1127	.536	1124:1127	The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures.					
32563954	7	73	dep	included	841:848	arg1	years					875:879	median age 28 years	861:879	median age 28 years	861:879	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
32563954	7	73	dep	included	841:848	arg1	males					854:858	12 males	851:858	12 males	851:858	In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm).					
34940975	6	0	theme	formulated	906:915	arg1	pasta					917:921	the optimally formulated pasta	892:921	the optimally formulated pasta	892:921	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	10	1	theme	PRACTICAL	1443:1451	arg1	APPLICATION					1453:1463	PRACTICAL APPLICATION	1443:1463	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.	1443:1623	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	2	theme	mixture	1600:1606	arg1	approach					1615:1622	a mixture design approach	1598:1622	a mixture design approach	1598:1622	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	1	3	theme	barley	227:232	arg1	flour					234:238	whole barley flour	221:238	whole barley flour	221:238	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	12	4	theme	human	1865:1869	arg1	diet					1871:1874	the human diet	1861:1874	the human diet	1861:1874	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	11	5	from	rich	1719:1722	arg1	fibers					1743:1748	several dietary fibers	1727:1748	several dietary fibers	1727:1748	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	10	6	theme	durum	1485:1489	arg1	semolina					1497:1504	durum wheat semolina	1485:1504	durum wheat semolina	1485:1504	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	6	theme	durum	1485:1489	arg1	ingredients					1472:1482	Three ingredients	1466:1482	Three ingredients	1466:1482	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	9	7	theme	few	1336:1338	arg1	granules					1363:1370	few not gelatinized starch granules	1336:1370	few not gelatinized starch granules	1336:1370	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	5	8	theme	flour	846:850	arg1	addition					852:859	whole barley flour addition	833:859	whole barley flour addition	833:859	However, pasta firmness was negatively influenced by inulin and whole barley flour addition.					
34940975	2	9	with	pasta	394:398	arg1	properties					443:452	acceptable technological and textural properties	405:452	acceptable technological and textural properties as close as possible	405:473	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta was studied.					
34940975	4	10	theme	barley	657:662	arg1	flour					664:668	whole barley flour and durum wheat semolina	651:693	flour	664:668	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	10	11	theme	whole	1507:1511	arg1	ingredients					1472:1482	Three ingredients	1466:1482	Three ingredients	1466:1482	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	11	theme	whole	1507:1511	arg1	flour					1520:1524	whole barley flour	1507:1524	whole barley flour	1507:1524	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	11	12	theme	textural	1694:1701	arg1	properties					1703:1712	good technological and textural properties	1671:1712	good technological and textural properties	1671:1712	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	3	13	theme	uncooked	556:563	arg1	pasta					565:569	uncooked pasta	556:569	uncooked pasta	556:569	The microstructure analysis of cooked and uncooked pasta was performed.					
34940975	9	14	theme	granules	1363:1370	arg1	presence					1324:1331	the presence	1320:1331	the presence of few not gelatinized starch granules	1320:1370	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	6	15	theme	technological	948:960	arg1	properties					975:984	the best technological and textural properties	939:984	the best technological and textural properties	939:984	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	11	16	theme	technological	1676:1688	arg1	properties					1703:1712	good technological and textural properties	1671:1712	good technological and textural properties	1671:1712	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	1	17	theme	mixture	348:354	arg1	approach					363:370	the mixture design approach	344:370	the mixture design approach	344:370	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	3	18	theme	cooked	545:550	arg1	analysis					533:540	The microstructure analysis	514:540	The microstructure analysis of cooked and uncooked pasta	514:569	The microstructure analysis of cooked and uncooked pasta was performed.					
34940975	1	19	theme	functional	171:180	arg1	pasta					182:186	a new functional pasta	165:186	a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients	165:262	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	9	20	dep	showed	1313:1318	arg1	incorporated					1372:1383	incorporated	1372:1383	showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta	1313:1440	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	4	21	theme	increasing	629:638	arg1	flour					664:668	whole barley flour and durum wheat semolina	651:693	flour	664:668	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	21	theme	increasing	629:638	arg1	semolina					686:693	whole barley flour and durum wheat semolina	651:693	semolina	686:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	21	theme	increasing	629:638	arg1	amounts					640:646	the increasing amounts	625:646	the increasing amounts of whole barley flour and durum wheat semolina	625:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	11	22	with	pasta	1659:1663	arg1	properties					1703:1712	good technological and textural properties	1671:1712	good technological and textural properties	1671:1712	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	5	23	theme	whole	833:837	arg1	addition					852:859	whole barley flour addition	833:859	whole barley flour addition	833:859	However, pasta firmness was negatively influenced by inulin and whole barley flour addition.					
34940975	8	24	theme	firmness	1188:1195	arg1	values					1197:1202	The firmness values	1184:1202	The firmness values	1184:1202	The firmness values and the color parameters were also satisfactory.					
34940975	8	24	theme	firmness	1188:1195	arg1	satisfactory					1239:1250	satisfactory	1239:1250	satisfactory	1239:1250	The firmness values and the color parameters were also satisfactory.					
34940975	1	25	theme	present	117:123	arg1	work					125:128	the present work	113:128	the present work	113:128	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	6	26	theme	durum	997:1001	arg1	semolina					1009:1016	durum wheat semolina	997:1016	94.8% durum wheat semolina	991:1016	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	8	27	theme	color	1212:1216	arg1	parameters					1218:1227	the color parameters	1208:1227	the color parameters	1208:1227	The firmness values and the color parameters were also satisfactory.					
34940975	8	27	theme	color	1212:1216	arg1	satisfactory					1239:1250	satisfactory	1239:1250	satisfactory	1239:1250	The firmness values and the color parameters were also satisfactory.					
34940975	0	28	theme	pasta	53:57	arg1	formulation					59:69	pasta formulation	53:69	pasta formulation using mixture design approach	53:99	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	9	29	theme	control	1428:1434	arg1	pasta					1436:1440	the control pasta	1424:1440	the control pasta	1424:1440	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	4	30	theme	wheat	680:684	arg1	semolina					686:693	whole barley flour and durum wheat semolina	651:693	semolina	686:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	0	31	theme	design	85:90	arg1	approach					92:99	mixture design approach	77:99	mixture design approach	77:99	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	6	32	theme	textural	966:973	arg1	properties					975:984	the best technological and textural properties	939:984	the best technological and textural properties	939:984	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	6	33	dep	%	1022:1022	arg1	flour					1037:1041	whole barley flour	1024:1041	3.7% whole barley flour	1019:1041	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	0	34	theme	inulin	33:38	arg1	addition					40:47	inulin addition	33:47	inulin addition	33:47	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	11	35	theme	formulated	1648:1657	arg1	rich					1719:1722	rich	1719:1722	rich	1719:1722	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	11	35	theme	formulated	1648:1657	arg1	pasta					1659:1663	The obtained optimally formulated pasta	1625:1663	The obtained optimally formulated pasta	1625:1663	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	2	36	theme	textural	434:441	arg1	properties					443:452	acceptable technological and textural properties	405:452	acceptable technological and textural properties as close as possible	405:473	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta was studied.					
34940975	7	37	theme	s	1124:1124	arg1	index					1138:1142	a swelling index	1127:1142	a swelling index of 2.15%	1127:1151	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	37	theme	s	1124:1124	arg1	loss					1168:1171	a cooking loss	1158:1171	a cooking loss of 10.44%	1158:1181	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	37	theme	s	1124:1124	arg1	time					1109:1112	an optimal cooking time	1090:1112	an optimal cooking time of 335.24 s	1090:1124	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	12	38	theme	barley	1788:1793	arg1	flour					1795:1799	whole barley flour	1782:1799	whole barley flour	1782:1799	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	6	39	theme	whole	1024:1028	arg1	flour					1037:1041	whole barley flour	1024:1041	3.7% whole barley flour	1019:1041	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	2	40	theme	technological	416:428	arg1	properties					443:452	acceptable technological and textural properties	405:452	acceptable technological and textural properties as close as possible	405:473	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta was studied.					
34940975	4	41	theme	cooking	726:732	arg1	parameters					742:751	the cooking quality parameters	722:751	the cooking quality parameters	722:751	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	10	42	theme	new	1571:1573	arg1	pasta					1586:1590	new functional pasta	1571:1590	new functional pasta using a mixture design approach	1571:1622	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	0	43	theme	barley	16:21	arg1	flour					23:27	barley flour	16:27	barley flour	16:27	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	2	44	theme	formulated	383:392	arg1	pasta					394:398	Optimally formulated pasta	373:398	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta	373:499	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta was studied.					
34940975	7	45	theme	cooking	1101:1107	arg1	time					1109:1112	an optimal cooking time	1090:1112	an optimal cooking time of 335.24 s	1090:1124	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	5	46	theme	pasta	778:782	arg1	firmness					784:791	pasta firmness	778:791	pasta firmness	778:791	However, pasta firmness was negatively influenced by inulin and whole barley flour addition.					
34940975	10	47	theme	pasta	1586:1590	arg1	production					1557:1566	the production	1553:1566	the production of new functional pasta using a mixture design approach	1553:1622	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	9	48	theme	formulated	1289:1298	arg1	pasta					1307:1311	the optimally formulated cooked pasta	1275:1311	the optimally formulated cooked pasta	1275:1311	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	4	49	theme	Cox	586:588	arg1	plots					605:609	Cox response trace plots	586:609	Cox response trace plots	586:609	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	9	50	theme	gelatinized	1344:1354	arg1	granules					1363:1370	few not gelatinized starch granules	1336:1370	few not gelatinized starch granules	1336:1370	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	9	51	theme	pasta	1307:1311	arg1	microstructure					1257:1270	The microstructure	1253:1270	The microstructure of the optimally formulated cooked pasta	1253:1311	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	6	52	theme	pasta	917:921	arg1	composition					877:887	The ingredient composition	862:887	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties,	862:985	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	6	52	theme	pasta	917:921	arg1	%					995:995	94.8%	991:995	94.8% durum wheat semolina	991:1016	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	4	53	theme	trace	599:603	arg1	plots					605:609	Cox response trace plots	586:609	Cox response trace plots	586:609	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	7	54	theme	optimal	1066:1072	arg1	formulation					1074:1084	This optimal formulation	1061:1084	This optimal formulation	1061:1084	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	10	55	theme	design	1608:1613	arg1	approach					1615:1622	a mixture design approach	1598:1622	a mixture design approach	1598:1622	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	1	56	theme	whole	221:225	arg1	flour					234:238	whole barley flour	221:238	whole barley flour	221:238	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	10	57	theme	wheat	1491:1495	arg1	semolina					1497:1504	durum wheat semolina	1485:1504	durum wheat semolina	1485:1504	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	57	theme	wheat	1491:1495	arg1	ingredients					1472:1482	Three ingredients	1466:1482	Three ingredients	1466:1482	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	7	58	contain	had	1086:1088	arg2	index					1138:1142	a swelling index	1127:1142	a swelling index of 2.15%	1127:1151	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	58	contain	had	1086:1088	arg2	loss					1168:1171	a cooking loss	1158:1171	a cooking loss of 10.44%	1158:1181	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	58	contain	had	1086:1088	arg2	time					1109:1112	an optimal cooking time	1090:1112	an optimal cooking time of 335.24 s	1090:1124	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	58	contain	had	1086:1088	arg1	formulation					1074:1084	This optimal formulation	1061:1084	This optimal formulation	1061:1084	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	3	59	theme	microstructure	518:531	arg1	analysis					533:540	The microstructure analysis	514:540	The microstructure analysis of cooked and uncooked pasta	514:569	The microstructure analysis of cooked and uncooked pasta was performed.					
34940975	5	60	theme	barley	839:844	arg1	addition					852:859	whole barley flour addition	833:859	whole barley flour addition	833:859	However, pasta firmness was negatively influenced by inulin and whole barley flour addition.					
34940975	0	61	theme	addition	40:47	arg1	Optimization					0:11	Optimization	0:11	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.	0:100	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	6	62	theme	ingredient	866:875	arg1	composition					877:887	The ingredient composition	862:887	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties,	862:985	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	6	62	theme	ingredient	866:875	arg1	%					995:995	94.8%	991:995	94.8% durum wheat semolina	991:1016	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	10	63	theme	barley	1513:1518	arg1	ingredients					1472:1482	Three ingredients	1466:1482	Three ingredients	1466:1482	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	63	theme	barley	1513:1518	arg1	flour					1520:1524	whole barley flour	1507:1524	whole barley flour	1507:1524	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	9	64	theme	starch	1356:1361	arg1	granules					1363:1370	few not gelatinized starch granules	1336:1370	few not gelatinized starch granules	1336:1370	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	12	65	from	application	1767:1777	arg1	industry					1826:1833	the cereal industry	1815:1833	the cereal industry	1815:1833	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	4	66	theme	whole	651:655	arg1	flour					664:668	whole barley flour and durum wheat semolina	651:693	flour	664:668	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	1	67	theme	durum	199:203	arg1	semolina					211:218	durum wheat semolina	199:218	durum wheat semolina	199:218	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	1	68	theme	new	167:169	arg1	pasta					182:186	a new functional pasta	165:186	a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients	165:262	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	1	69	theme	design	356:361	arg1	approach					363:370	the mixture design approach	344:370	the mixture design approach	344:370	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	1	70	theme	pasta	182:186	arg1	formulation					150:160	the formulation	146:160	the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach	146:370	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	12	71	theme	cereal	1819:1824	arg1	industry					1826:1833	the cereal industry	1815:1833	the cereal industry	1815:1833	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	10	72	dep	APPLICATION	1453:1463	arg1	used					1544:1547	used	1544:1547	were used for the production of new functional pasta using a mixture design approach	1539:1622	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	3	73	theme	pasta	565:569	arg1	analysis					533:540	The microstructure analysis	514:540	The microstructure analysis of cooked and uncooked pasta	514:569	The microstructure analysis of cooked and uncooked pasta was performed.					
34940975	4	74	theme	semolina	686:693	arg1	flour					664:668	whole barley flour and durum wheat semolina	651:693	flour	664:668	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	74	theme	semolina	686:693	arg1	semolina					686:693	whole barley flour and durum wheat semolina	651:693	semolina	686:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	74	theme	semolina	686:693	arg1	amounts					640:646	the increasing amounts	625:646	the increasing amounts of whole barley flour and durum wheat semolina	625:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	6	75	theme	wheat	1003:1007	arg1	semolina					1009:1016	durum wheat semolina	997:1016	94.8% durum wheat semolina	991:1016	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	11	76	theme	several	1727:1733	arg1	fibers					1743:1748	several dietary fibers	1727:1748	several dietary fibers	1727:1748	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	7	77	theme	%	1151:1151	arg1	index					1138:1142	a swelling index	1127:1142	a swelling index of 2.15%	1127:1151	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	77	theme	%	1151:1151	arg1	loss					1168:1171	a cooking loss	1158:1171	a cooking loss of 10.44%	1158:1181	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	77	theme	%	1151:1151	arg1	time					1109:1112	an optimal cooking time	1090:1112	an optimal cooking time of 335.24 s	1090:1124	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	1	78	theme	work	125:128	arg1	aim					106:108	The aim	102:108	The aim of the present work	102:128	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	4	79	theme	flour	664:668	arg1	flour					664:668	whole barley flour and durum wheat semolina	651:693	flour	664:668	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	79	theme	flour	664:668	arg1	semolina					686:693	whole barley flour and durum wheat semolina	651:693	semolina	686:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	79	theme	flour	664:668	arg1	amounts					640:646	the increasing amounts	625:646	the increasing amounts of whole barley flour and durum wheat semolina	625:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	11	80	theme	obtained	1629:1636	arg1	rich					1719:1722	rich	1719:1722	rich	1719:1722	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	11	80	theme	obtained	1629:1636	arg1	pasta					1659:1663	The obtained optimally formulated pasta	1625:1663	The obtained optimally formulated pasta	1625:1663	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	0	81	theme	mixture	77:83	arg1	approach					92:99	mixture design approach	77:99	mixture design approach	77:99	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	1	82	theme	textural	302:309	arg1	properties					311:320	both the technological and textural properties	275:320	properties	311:320	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	12	83	theme	inulin	1805:1810	arg1	application					1767:1777	the application	1763:1777	the application of whole barley flour and inulin in the cereal industry	1763:1833	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	4	84	theme	durum	674:678	arg1	semolina					686:693	whole barley flour and durum wheat semolina	651:693	semolina	686:693	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	11	85	theme	good	1671:1674	arg1	properties					1703:1712	good technological and textural properties	1671:1712	good technological and textural properties	1671:1712	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	7	86	theme	swelling	1129:1136	arg1	index					1138:1142	a swelling index	1127:1142	a swelling index of 2.15%	1127:1151	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	6	87	dep	%	995:995	arg1	semolina					1009:1016	durum wheat semolina	997:1016	94.8% durum wheat semolina	991:1016	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	4	88	theme	quality	734:740	arg1	parameters					742:751	the cooking quality parameters	722:751	the cooking quality parameters	722:751	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	11	89	theme	dietary	1735:1741	arg1	fibers					1743:1748	several dietary fibers	1727:1748	several dietary fibers	1727:1748	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	1	90	theme	product	330:336	arg1	properties					311:320	both the technological and textural properties	275:320	properties	311:320	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	12	91	theme	whole	1782:1786	arg1	flour					1795:1799	whole barley flour	1782:1799	whole barley flour	1782:1799	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	2	92	theme	control	487:493	arg1	pasta					495:499	control pasta	487:499	control pasta	487:499	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta was studied.					
34940975	1	93	theme	wheat	205:209	arg1	semolina					211:218	durum wheat semolina	199:218	durum wheat semolina	199:218	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	12	94	theme	flour	1795:1799	arg1	application					1767:1777	the application	1763:1777	the application of whole barley flour and inulin in the cereal industry	1763:1833	This allows the application of whole barley flour and inulin in the cereal industry and can be of interest to the human diet.					
34940975	6	95	theme	barley	1030:1035	arg1	flour					1037:1041	whole barley flour	1024:1041	3.7% whole barley flour	1019:1041	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	1	96	dep	properties	311:320	arg1	both					275:278	both	275:278	both	275:278	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	11	97	from	fibers	1743:1748	arg1	rich					1719:1722	rich	1719:1722	rich	1719:1722	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	11	97	from	fibers	1743:1748	arg1	pasta					1659:1663	The obtained optimally formulated pasta	1625:1663	The obtained optimally formulated pasta	1625:1663	The obtained optimally formulated pasta, with good technological and textural properties, was rich in several dietary fibers.					
34940975	0	98	theme	flour	23:27	arg1	Optimization					0:11	Optimization	0:11	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.	0:100	Optimization of barley flour and inulin addition for pasta formulation using mixture design approach.					
34940975	1	99	theme	inulin	245:250	arg1	ingredients					252:262	inulin ingredients	245:262	inulin ingredients	245:262	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	4	100	from	increase	710:717	arg1	parameters					742:751	the cooking quality parameters	722:751	the cooking quality parameters	722:751	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	4	100	from	increase	710:717	arg1	yellowness					757:766	yellowness	757:766	yellowness	757:766	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	10	101	used	used	1544:1547	arg2	inulin					1531:1536	inulin	1531:1536	inulin	1531:1536	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	101	used	used	1544:1547	arg2	semolina					1497:1504	durum wheat semolina	1485:1504	durum wheat semolina	1485:1504	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	101	used	used	1544:1547	arg2	ingredients					1472:1482	Three ingredients	1466:1482	Three ingredients	1466:1482	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	10	101	used	used	1544:1547	arg2	flour					1520:1524	whole barley flour	1507:1524	whole barley flour	1507:1524	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	2	102	theme	acceptable	405:414	arg1	properties					443:452	acceptable technological and textural properties	405:452	acceptable technological and textural properties as close as possible	405:473	Optimally formulated pasta with acceptable technological and textural properties as close as possible to those of control pasta was studied.					
34940975	7	103	theme	optimal	1093:1099	arg1	time					1109:1112	an optimal cooking time	1090:1112	an optimal cooking time of 335.24 s	1090:1124	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	104	theme	%	1181:1181	arg1	index					1138:1142	a swelling index	1127:1142	a swelling index of 2.15%	1127:1151	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	104	theme	%	1181:1181	arg1	loss					1168:1171	a cooking loss	1158:1171	a cooking loss of 10.44%	1158:1181	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	7	104	theme	%	1181:1181	arg1	time					1109:1112	an optimal cooking time	1090:1112	an optimal cooking time of 335.24 s	1090:1124	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
34940975	1	105	contain	containing	188:197	arg2	ingredients					252:262	inulin ingredients	245:262	inulin ingredients	245:262	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	1	105	contain	containing	188:197	arg2	flour					234:238	whole barley flour	221:238	whole barley flour	221:238	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	1	105	contain	containing	188:197	arg1	pasta					182:186	a new functional pasta	165:186	a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients	165:262	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	1	105	contain	containing	188:197	arg2	semolina					211:218	durum wheat semolina	199:218	durum wheat semolina	199:218	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	6	106	dep	%	1051:1051	arg1	inulin					1053:1058	inulin	1053:1058	1.5% inulin	1048:1058	The ingredient composition of the optimally formulated pasta, which leads to the best technological and textural properties, was 94.8% durum wheat semolina, 3.7% whole barley flour, and 1.5% inulin.					
34940975	1	107	theme	technological	284:296	arg1	properties					311:320	both the technological and textural properties	275:320	properties	311:320	The aim of the present work was to optimize the formulation of a new functional pasta containing durum wheat semolina, whole barley flour, and inulin ingredients to enhance both the technological and textural properties of this product using the mixture design approach.					
34940975	4	108	theme	response	590:597	arg1	plots					605:609	Cox response trace plots	586:609	Cox response trace plots	586:609	Cox response trace plots revealed that the increasing amounts of whole barley flour and durum wheat semolina resulted in an increase in the cooking quality parameters and yellowness.					
34940975	10	109	theme	functional	1575:1584	arg1	pasta					1586:1590	new functional pasta	1571:1590	new functional pasta using a mixture design approach	1571:1622	PRACTICAL APPLICATION: Three ingredients, durum wheat semolina, whole barley flour, and inulin, were used for the production of new functional pasta using a mixture design approach.					
34940975	9	110	theme	cooked	1300:1305	arg1	pasta					1307:1311	the optimally formulated cooked pasta	1275:1311	the optimally formulated cooked pasta	1275:1311	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	9	111	theme	protein	1394:1400	arg1	matrix					1402:1407	the protein matrix	1390:1407	the protein matrix	1390:1407	The microstructure of the optimally formulated cooked pasta showed the presence of few not gelatinized starch granules incorporated into the protein matrix as compared to the control pasta.					
34940975	7	112	theme	cooking	1160:1166	arg1	loss					1168:1171	a cooking loss	1158:1171	a cooking loss of 10.44%	1158:1181	This optimal formulation had an optimal cooking time of 335.24 s, a swelling index of 2.15%, and a cooking loss of 10.44%.					
32805289	0	0	theme	conductive	67:76	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	5	1	dep	strength	1003:1010	arg1	the					991:993	the	991:993	the	991:993	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	7	2	theme	high-performance	1301:1316	arg1	electrolyte					1333:1343	the high-performance organohydrogel electrolyte	1297:1343	the high-performance organohydrogel electrolyte	1297:1343	This paper provides a new preparation method for the high-performance organohydrogel electrolyte.					
32805289	5	3	theme	high	971:974	arg1	toughness					976:984	the high toughness	967:984	the high toughness	967:984	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	5	4	from	break	1030:1034	arg1	elongation					1016:1025	elongation	1016:1025	elongation	1016:1025	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	5	4	from	break	1030:1034	arg1	strength					1003:1010	tensile strength	995:1010	tensile strength	995:1010	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	0	5	theme	thermoplastic	82:94	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	3	6	theme	water	603:607	arg1	solution					581:588	a mixed solution	573:588	a mixed solution of distilled water and glycerol	573:620	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	7	7	theme	preparation	1274:1284	arg1	method					1286:1291	a new preparation method	1268:1291	a new preparation method for the high-performance organohydrogel electrolyte	1268:1343	This paper provides a new preparation method for the high-performance organohydrogel electrolyte.					
32805289	3	8	theme	NaCl	765:768	arg1	solution					778:785	the saturated NaCl aqueous solution	751:785	the saturated NaCl aqueous solution	751:785	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	4	9	theme	soaking	799:805	arg1	process					807:813	the soaking process	795:813	the soaking process	795:813	During the soaking process NaCl would enter into the PVA/SA/Gly organohydrogel to increase the gel strength and conductivity.					
32805289	7	10	theme	new	1270:1272	arg1	method					1286:1291	a new preparation method	1268:1291	a new preparation method for the high-performance organohydrogel electrolyte	1268:1343	This paper provides a new preparation method for the high-performance organohydrogel electrolyte.					
32805289	3	11	theme	aqueous	770:776	arg1	solution					778:785	the saturated NaCl aqueous solution	751:785	the saturated NaCl aqueous solution	751:785	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	5	12	theme	1.43 MPa	1039:1046	arg1	elongation					1016:1025	elongation	1016:1025	elongation	1016:1025	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	5	12	theme	1.43 MPa	1039:1046	arg1	strength					1003:1010	tensile strength	995:1010	tensile strength	995:1010	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	1	13	theme	Facile	169:174	arg1	preparation					176:186	Facile preparation	169:186	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity	169:320	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity is important and still remains challenging.					
32805289	0	14	theme	poly	96:99	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	2	15	from	method	515:520	arg1	paper					530:534	this paper	525:534	this paper	525:534	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	4	16	theme	gel	883:885	arg1	strength					887:894	the gel strength	879:894	the gel strength	879:894	During the soaking process NaCl would enter into the PVA/SA/Gly organohydrogel to increase the gel strength and conductivity.					
32805289	0	17	theme	facile	2:7	arg1	method					21:26	A facile preparation method	0:26	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.	0:167	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	3	18	theme	saturated	755:763	arg1	solution					778:785	the saturated NaCl aqueous solution	751:785	the saturated NaCl aqueous solution	751:785	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	7	19	theme	organohydrogel	1318:1331	arg1	electrolyte					1333:1343	the high-performance organohydrogel electrolyte	1297:1343	the high-performance organohydrogel electrolyte	1297:1343	This paper provides a new preparation method for the high-performance organohydrogel electrolyte.					
32805289	5	20	theme	PVA/SA/Gly	918:927	arg1	electrolytes					944:955	The PVA/SA/Gly organohydrogel electrolytes	914:955	The PVA/SA/Gly organohydrogel electrolytes	914:955	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	3	21	theme	distilled	593:601	arg1	water					603:607	distilled water	593:607	distilled water	593:607	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	1	22	theme	organohydrogel	191:204	arg1	electrolyte					206:216	organohydrogel electrolyte	191:216	organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity	191:320	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity is important and still remains challenging.					
32805289	0	23	theme	alcohol	107:113	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	3	24	theme	mixed	575:579	arg1	solution					581:588	a mixed solution	573:588	a mixed solution of distilled water and glycerol	573:620	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	1	25	theme	electrolyte	206:216	arg1	preparation					176:186	Facile preparation	169:186	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity	169:320	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity is important and still remains challenging.					
32805289	2	26	theme	organohydrogel	461:474	arg1	electrolytes					476:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	0	27	theme	vinyl	101:105	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	6	28	theme	glycerol	1238:1245	arg1	incorporation					1221:1233	the incorporation	1217:1233	the incorporation of glycerol	1217:1245	Moreover, the PVA/SA/Gly organohydrogel electrolytes behaved high transparency, anti-freezing property, conductivity and thermoplasticity due to the incorporation of glycerol.					
32805289	0	29	theme	anti-freezing	32:44	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	0	30	theme	/sodium	115:121	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	3	31	theme	freezing-thawing	676:691	arg1	process					693:699	the freezing-thawing process	672:699	the freezing-thawing process	672:699	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	3	32	theme	glycerol	613:620	arg1	solution					581:588	a mixed solution	573:588	a mixed solution of distilled water and glycerol	573:620	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	5	33	theme	organohydrogel	929:942	arg1	electrolytes					944:955	The PVA/SA/Gly organohydrogel electrolytes	914:955	The PVA/SA/Gly organohydrogel electrolytes	914:955	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	6	34	theme	organohydrogel	1097:1110	arg1	electrolytes					1112:1123	the PVA/SA/Gly organohydrogel electrolytes	1082:1123	the PVA/SA/Gly organohydrogel electrolytes	1082:1123	Moreover, the PVA/SA/Gly organohydrogel electrolytes behaved high transparency, anti-freezing property, conductivity and thermoplasticity due to the incorporation of glycerol.					
32805289	2	35	theme	simple	508:513	arg1	method					515:520	a simple method	506:520	a simple method in this paper	506:534	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	1	36	theme	good	234:237	arg1	property					253:260	good anti-freezing property	234:260	good anti-freezing property	234:260	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity is important and still remains challenging.					
32805289	0	37	theme	tough	47:51	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	0	38	theme	organohydrogel	141:154	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	2	39	dep	composite	451:459	arg1	alcohol					404:410	vinyl alcohol	398:410	vinyl alcohol	398:410	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	6	40	theme	high	1133:1136	arg1	transparency					1138:1149	high transparency	1133:1149	high transparency	1133:1149	Moreover, the PVA/SA/Gly organohydrogel electrolytes behaved high transparency, anti-freezing property, conductivity and thermoplasticity due to the incorporation of glycerol.					
32805289	0	41	theme	alginate/glycerol	123:139	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	2	42	theme	conductive	372:381	arg1	electrolytes					476:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	3	43	theme	PVA/SA/Gly	630:639	arg1	organohydrogel					641:654	the PVA/SA/Gly organohydrogel	626:654	the PVA/SA/Gly organohydrogel	626:654	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	3	44	theme	PVA/SA/Gly	707:716	arg1	organohydrogel					718:731	PVA/SA/Gly organohydrogel	707:731	PVA/SA/Gly organohydrogel	707:731	PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution.					
32805289	2	45	theme	Novel	366:370	arg1	electrolytes					476:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	1	46	theme	anti-freezing	239:251	arg1	property					253:260	good anti-freezing property	234:260	good anti-freezing property	234:260	Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity is important and still remains challenging.					
32805289	5	47	theme	%	1055:1055	arg1	elongation					1016:1025	elongation	1016:1025	elongation	1016:1025	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	5	47	theme	%	1055:1055	arg1	strength					1003:1010	tensile strength	995:1010	tensile strength	995:1010	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	5	48	theme	tensile	995:1001	arg1	strength					1003:1010	tensile strength	995:1010	tensile strength	995:1010	The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively.					
32805289	0	49	theme	transparent	54:64	arg1	electrolyte					156:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte	32:166	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
32805289	6	50	theme	PVA/SA/Gly	1086:1095	arg1	electrolytes					1112:1123	the PVA/SA/Gly organohydrogel electrolytes	1082:1123	the PVA/SA/Gly organohydrogel electrolytes	1082:1123	Moreover, the PVA/SA/Gly organohydrogel electrolytes behaved high transparency, anti-freezing property, conductivity and thermoplasticity due to the incorporation of glycerol.					
32805289	4	51	theme	PVA/SA/Gly	841:850	arg1	organohydrogel					852:865	the PVA/SA/Gly organohydrogel	837:865	the PVA/SA/Gly organohydrogel	837:865	During the soaking process NaCl would enter into the PVA/SA/Gly organohydrogel to increase the gel strength and conductivity.					
32805289	2	52	theme	composite	451:459	arg1	electrolytes					476:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes	366:487	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	2	53	theme	vinyl	398:402	arg1	alcohol					404:410	vinyl alcohol	398:410	vinyl alcohol	398:410	Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper.					
32805289	0	54	theme	preparation	9:19	arg1	method					21:26	A facile preparation method	0:26	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.	0:167	A facile preparation method for anti-freezing, tough, transparent, conductive and thermoplastic poly(vinyl alcohol)/sodium alginate/glycerol organohydrogel electrolyte.					
34500211	7	0	theme	specific	1062:1069	arg1	traits					1094:1099	specific kernel physicochemical traits	1062:1099	specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI	1062:1173	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	3	1	dep	correlated	593:602	arg1	p < 0.05					605:612	p < 0.05	605:612	p < 0.05	605:612	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	4	2	theme	bonds	838:842	arg1	impact					818:823	the impact	814:823	the impact of disulfide bonds on this attribute	814:860	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	1	3	from	source	149:154	arg1	diet					165:168	human diet	159:168	human diet	159:168	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	3	4	dep	ones	532:535	arg1	80.7					547:550	80.7	547:550	80.7	547:550	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	4	dep	ones	532:535	arg1	70.7					538:541	70.7	538:541	70.7	538:541	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	0	5	theme	maize	93:97	arg1	flours					99:104	cooked maize flours	86:104	cooked maize flours	86:104	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	3	6	theme	specific	620:627	arg1	C1					675:676	C1	675:676	C1	675:676	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	6	theme	specific	620:627	arg1	concentrations					634:647	specific zein concentrations	620:647	specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins)	620:693	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	6	theme	specific	620:627	arg1	E					679:679	E	679:679	E	679:679	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	4	7	theme	starch	760:765	arg1	hydrolysis					767:776	starch hydrolysis	760:776	starch hydrolysis	760:776	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	2	8	theme	starch	274:279	arg1	digestibility					281:293	in vitro starch digestibility	265:293	in vitro starch digestibility	265:293	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	0	9	theme	cooked	86:91	arg1	flours					99:104	cooked maize flours	86:104	cooked maize flours	86:104	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	4	10	theme	disulfide	828:836	arg1	bonds					838:842	disulfide bonds	828:842	disulfide bonds	828:842	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	3	11	theme	hard	464:467	arg1	genotypes					479:487	hard endosperm genotypes	464:487	hard endosperm genotypes	464:487	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	7	12	with	products	1127:1134	arg1	GI					1172:1173	GI	1172:1173	GI	1172:1173	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	7	12	with	products	1127:1134	arg1	digestibility					1154:1166	lower starch digestibility	1141:1166	lower starch digestibility	1141:1166	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	2	13	from	hardness	325:332	arg1	flours					366:371	cooked maize flours	353:371	cooked maize flours	353:371	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	2	14	theme	in	265:266	arg1	digestibility					281:293	in vitro starch digestibility	265:293	in vitro starch digestibility	265:293	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	1	15	theme	human	159:163	arg1	diet					165:168	human diet	159:168	human diet	159:168	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	0	16	theme	flours	99:104	arg1	index					77:81	glycemic index	68:81	glycemic index of cooked maize flours	68:104	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	0	16	theme	flours	99:104	arg1	digestibility					50:62	starch digestibility	43:62	starch digestibility	43:62	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	4	17	theme	sodium	709:714	arg1	sulfite					716:722	sodium sulfite	709:722	sodium sulfite	709:722	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	4	18	with	Cooking	696:702	arg1	sulfite					716:722	sodium sulfite	709:722	sodium sulfite	709:722	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	3	19	theme	Starch	374:379	arg1	digestion					381:389	Starch digestion	374:389	Starch digestion	374:389	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	20	theme	total	650:654	arg1	zeins					656:660	total zeins	650:660	total zeins	650:660	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	7	21	theme	kernel	1071:1076	arg1	traits					1094:1099	specific kernel physicochemical traits	1062:1099	specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI	1062:1173	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	2	22	from	composition	338:348	arg1	flours					366:371	cooked maize flours	353:371	cooked maize flours	353:371	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	3	23	theme	soft	527:530	arg1	ones					532:535	soft ones	527:535	soft ones (70.7 and 80.7, respectively)	527:565	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	0	24	theme	Physical	0:7	arg1	traits					29:34	Physical and chemical kernel traits	0:34	Physical and chemical kernel traits	0:34	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	4	25	from	impact	818:823	arg1	attribute					852:860	this attribute	847:860	this attribute	847:860	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	6	26	theme	starch	1011:1016	arg1	digestibility					1018:1030	total starch digestibility	1005:1030	total starch digestibility in >30%	1005:1038	Regardless of hardness, fine grinding significantly (p < 0.001) increased total starch digestibility in >30%.					
34500211	0	27	theme	kernel	22:27	arg1	traits					29:34	Physical and chemical kernel traits	0:34	Physical and chemical kernel traits	0:34	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	6	28	theme	total	1005:1009	arg1	digestibility					1018:1030	total starch digestibility	1005:1030	total starch digestibility in >30%	1005:1038	Regardless of hardness, fine grinding significantly (p < 0.001) increased total starch digestibility in >30%.					
34500211	0	29	theme	chemical	13:20	arg1	traits					29:34	Physical and chemical kernel traits	0:34	Physical and chemical kernel traits	0:34	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	6	30	from	digestibility	1018:1030	arg1	%					1038:1038	>30%	1035:1038	>30%	1035:1038	Regardless of hardness, fine grinding significantly (p < 0.001) increased total starch digestibility in >30%.					
34500211	1	31	theme	important	126:134	arg1	source					149:154	an important carbohydrate source	123:154	an important carbohydrate source in human diet	123:168	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	1	31	theme	important	126:134	arg1	starch					113:118	Maize starch	107:118	Maize starch	107:118	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	3	32	theme	glycemic	405:412	arg1	GI					421:422	GI	421:422	GI	421:422	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	32	theme	glycemic	405:412	arg1	index					414:418	estimated glycemic index	395:418	estimated glycemic index (GI)	395:423	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	33	dep	concentrations	634:647	arg1	Z2					667:668	Z2	667:668	Z2	667:668	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	33	dep	concentrations	634:647	arg1	C1					675:676	C1	675:676	C1	675:676	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	33	dep	concentrations	634:647	arg1	Z1					663:664	Z1	663:664	Z1	663:664	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	33	dep	concentrations	634:647	arg1	concentrations					634:647	specific zein concentrations	620:647	specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins)	620:693	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	33	dep	concentrations	634:647	arg1	zeins					656:660	total zeins	650:660	total zeins	650:660	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	7	34	theme	maize	1116:1120	arg1	products					1127:1134	maize food products	1116:1134	maize food products with lower starch digestibility and GI	1116:1173	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	5	35	theme	amylose	872:878	arg1	ratios					887:892	amylose:starch ratios	872:892	Explored amylose:starch ratios	863:892	Explored amylose:starch ratios did not impact starch digestibility.					
34500211	6	36	dep	significantly	969:981	arg1	p < 0.001					984:992	p < 0.001	984:992	p < 0.001	984:992	Regardless of hardness, fine grinding significantly (p < 0.001) increased total starch digestibility in >30%.					
34500211	7	37	theme	physicochemical	1078:1092	arg1	traits					1094:1099	specific kernel physicochemical traits	1062:1099	specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI	1062:1173	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	5	38	theme	starch	880:885	arg1	ratios					887:892	amylose:starch ratios	872:892	Explored amylose:starch ratios	863:892	Explored amylose:starch ratios did not impact starch digestibility.					
34500211	1	39	theme	Maize	107:111	arg1	source					149:154	an important carbohydrate source	123:154	an important carbohydrate source in human diet	123:168	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	1	39	theme	Maize	107:111	arg1	starch					113:118	Maize starch	107:118	Maize starch	107:118	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	0	40	theme	starch	43:48	arg1	digestibility					50:62	starch digestibility	43:62	starch digestibility	43:62	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	2	41	dep	in	265:266	arg1	vitro					268:272	vitro	268:272	vitro	268:272	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	3	42	theme	estimated	395:403	arg1	GI					421:422	GI	421:422	GI	421:422	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	42	theme	estimated	395:403	arg1	index					414:418	estimated glycemic index	395:418	estimated glycemic index (GI)	395:423	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	7	43	theme	lower	1141:1145	arg1	digestibility					1154:1166	lower starch digestibility	1141:1166	lower starch digestibility	1141:1166	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	3	44	theme	endosperm	469:477	arg1	genotypes					479:487	hard endosperm genotypes	464:487	hard endosperm genotypes	464:487	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	7	45	theme	food	1122:1125	arg1	products					1127:1134	maize food products	1116:1134	maize food products with lower starch digestibility and GI	1116:1173	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	2	46	theme	endosperm	315:323	arg1	hardness					325:332	endosperm hardness	315:332	endosperm hardness	315:332	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	3	47	theme	F	686:686	arg1	zeins					688:692	F zeins	686:692	F zeins	686:692	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	7	48	theme	starch	1147:1152	arg1	digestibility					1154:1166	lower starch digestibility	1141:1166	lower starch digestibility	1141:1166	Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.					
34500211	3	49	dep	lower	455:459	arg1	p < 0.05					445:452	p < 0.05	445:452	p < 0.05	445:452	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	49	dep	lower	455:459	arg1	77.3					499:502	77.3	499:502	77.3	499:502	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	49	dep	lower	455:459	arg1	65.1					490:493	65.1	490:493	65.1	490:493	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	1	50	theme	carbohydrate	136:147	arg1	source					149:154	an important carbohydrate source	123:154	an important carbohydrate source in human diet	123:168	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	1	50	theme	carbohydrate	136:147	arg1	starch					113:118	Maize starch	107:118	Maize starch	107:118	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	1	51	theme	postprandial	208:219	arg1	level					235:239	the postprandial blood glucose level	204:239	the postprandial blood glucose level	204:239	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	0	52	theme	glycemic	68:75	arg1	index					77:81	glycemic index	68:81	glycemic index of cooked maize flours	68:104	Physical and chemical kernel traits affect starch digestibility and glycemic index of cooked maize flours.					
34500211	5	53	theme	starch	909:914	arg1	digestibility					916:928	starch digestibility	909:928	starch digestibility	909:928	Explored amylose:starch ratios did not impact starch digestibility.					
34500211	2	54	theme	maize	360:364	arg1	flours					366:371	cooked maize flours	353:371	cooked maize flours	353:371	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	1	55	theme	blood	221:225	arg1	level					235:239	the postprandial blood glucose level	204:239	the postprandial blood glucose level	204:239	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
34500211	4	56	dep	significantly	724:736	arg1	p < 0.001					739:747	p < 0.001	739:747	p < 0.001	739:747	Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (∼13%), evidencing the impact of disulfide bonds on this attribute.					
34500211	3	57	theme	zein	629:632	arg1	C1					675:676	C1	675:676	C1	675:676	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	57	theme	zein	629:632	arg1	concentrations					634:647	specific zein concentrations	620:647	specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins)	620:693	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	3	57	theme	zein	629:632	arg1	E					679:679	E	679:679	E	679:679	Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins).					
34500211	5	58	theme	Explored	863:870	arg1	ratios					887:892	amylose:starch ratios	872:892	Explored amylose:starch ratios	863:892	Explored amylose:starch ratios did not impact starch digestibility.					
34500211	2	59	theme	cooked	353:358	arg1	flours					366:371	cooked maize flours	353:371	cooked maize flours	353:371	This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours.					
34500211	1	60	theme	glucose	227:233	arg1	level					235:239	the postprandial blood glucose level	204:239	the postprandial blood glucose level	204:239	Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level.					
32090575	8	0	from	glycans	1271:1277	arg1	peptides					1329:1336	peptides	1329:1336	peptides	1329:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	8	1	from	distributions	1254:1266	arg1	peptides					1329:1336	peptides	1329:1336	peptides	1329:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	7	2	theme	data	1187:1190	arg1	glycopeptides					1219:1231	endogenously intact glycopeptides	1199:1231	endogenously intact glycopeptides reported so far	1199:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	2	theme	data	1187:1190	arg1	set					1192:1194	the largest data set	1175:1194	the largest data set of endogenously intact glycopeptides reported so far	1175:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	4	3	from	amount	732:737	arg1	glycopeptides					705:717	the endogenously intact glycopeptides	681:717	the endogenously intact glycopeptides from a small amount of serum	681:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	3	from	amount	732:737	arg1	extraction					667:676	the highly efficient extraction	646:676	the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum	646:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	3	4	link	N-linked	439:446	arg1	glycopeptides					481:493	N-linked and O-linked endogenously intact glycopeptides	439:493	N-linked and O-linked endogenously intact glycopeptides	439:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	8	5	theme	sites	1320:1324	arg1	distributions					1254:1266	The distributions	1250:1266	The distributions of glycans among peptides and proteins and cleavage sites on peptides	1250:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	1	6	theme	biomarker	181:189	arg1	discovery					191:199	biomarker discovery	181:199	biomarker discovery	181:199	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	3	7	link	O-linked	452:459	arg1	glycopeptides					481:493	N-linked and O-linked endogenously intact glycopeptides	439:493	N-linked and O-linked endogenously intact glycopeptides	439:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	4	8	gly	glycopeptides	705:717	arg2	glycopeptides					705:717	the endogenously intact glycopeptides	681:717	the endogenously intact glycopeptides from a small amount of serum	681:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	8	gly	glycopeptides	705:717	arg1	serum					742:746	serum	742:746	serum	742:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	8	gly	glycopeptides	705:717	arg1	amount					732:737	a small amount	724:737	a small amount of serum	724:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	2	9	theme	intact	318:323	arg1	glycopeptides					325:337	endogenously intact glycopeptides	305:337	endogenously intact glycopeptides	305:337	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	6	10	theme	serum	1038:1042	arg1	sample					1044:1049	the human serum sample	1028:1049	the human serum sample	1028:1049	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	7	11	theme	glycopeptide	1150:1161	arg1	identification					1090:1103	the identification	1086:1103	the identification of endogenously intact N-linked and O-linked glycopeptide	1086:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	0	12	from	Serum	80:84	arg1	Profiling					0:8	Profiling	0:8	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.	0:114	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	4	13	theme	glycopeptides	705:717	arg1	extraction					667:676	the highly efficient extraction	646:676	the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum	646:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	5	14	theme	endogenous	887:896	arg1	glycopeptides					898:910	N-linked and O-linked endogenous glycopeptides	865:910	N-linked and O-linked endogenous glycopeptides	865:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	8	15	from	peptides	1329:1336	arg1	distributions					1254:1266	The distributions	1250:1266	The distributions of glycans among peptides and proteins and cleavage sites on peptides	1250:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	7	16	theme	intact	1121:1126	arg1	glycopeptide					1150:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	17	theme	first	1064:1068	arg1	study					1070:1074	the first study	1060:1074	the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide	1060:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	17	theme	first	1064:1068	arg1	This					1052:1055	This	1052:1055	This	1052:1055	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	18	theme	intact	1212:1217	arg1	glycopeptides					1219:1231	endogenously intact glycopeptides	1199:1231	endogenously intact glycopeptides reported so far	1199:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	2	19	theme	tandem	257:262	arg1	spectrometry					269:280	tandem mass spectrometry	257:280	tandem mass spectrometry	257:280	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	7	20	gly	glycopeptide	1150:1161	arg2	glycopeptide					1150:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	4	21	from	extraction	667:676	arg1	serum					742:746	serum	742:746	serum	742:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	21	from	extraction	667:676	arg1	amount					732:737	a small amount	724:737	a small amount of serum	724:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	5	22	link	O-linked	878:885	arg1	glycopeptides					898:910	N-linked and O-linked endogenous glycopeptides	865:910	N-linked and O-linked endogenous glycopeptides	865:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	4	23	theme	high-temperature	518:533	arg1	denaturation					540:551	the high-temperature acid denaturation	514:551	the high-temperature acid denaturation	514:551	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	8	24	theme	disease	1414:1420	arg1	mechanism					1422:1430	disease mechanism	1414:1430	disease mechanism	1414:1430	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	5	25	theme	in	791:792	arg1	strategy					817:824	in silico deglycosylation strategy	791:824	in silico deglycosylation strategy	791:824	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	3	26	theme	O-linked	452:459	arg1	glycopeptides					481:493	N-linked and O-linked endogenously intact glycopeptides	439:493	N-linked and O-linked endogenously intact glycopeptides	439:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	5	27	theme	deglycosylation	801:815	arg1	strategy					817:824	in silico deglycosylation strategy	791:824	in silico deglycosylation strategy	791:824	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	3	28	theme	N-linked	439:446	arg1	glycopeptides					481:493	N-linked and O-linked endogenously intact glycopeptides	439:493	N-linked and O-linked endogenously intact glycopeptides	439:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	5	29	theme	O-linked	878:885	arg1	glycopeptides					898:910	N-linked and O-linked endogenous glycopeptides	865:910	N-linked and O-linked endogenous glycopeptides	865:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	3	30	theme	integrated	379:388	arg1	platform					390:397	an integrated platform	376:397	an integrated platform	376:397	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	5	31	theme	glycopeptides	898:910	arg1	identification					847:860	the identification	843:860	the identification of N-linked and O-linked endogenous glycopeptides	843:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	6	32	theme	intact	941:946	arg1	N-glycopeptides					948:962	223 intact N-glycopeptides	937:962	223 intact N-glycopeptides	937:962	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	8	33	theme	glycosylation	1396:1408	arg1	regulation					1371:1380	the regulation	1367:1380	the regulation of endogenous glycosylation	1367:1408	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	3	34	theme	intact	474:479	arg1	glycopeptides					481:493	N-linked and O-linked endogenously intact glycopeptides	439:493	N-linked and O-linked endogenously intact glycopeptides	439:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	0	35	theme	N-Linked	33:40	arg1	Glycopeptides					55:67	Endogenously Intact N-Linked and O-Linked Glycopeptides	13:67	Endogenously Intact N-Linked and O-Linked Glycopeptides	13:67	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	5	36	gly	glycopeptides	898:910	arg2	glycopeptides					898:910	N-linked and O-linked endogenous glycopeptides	865:910	N-linked and O-linked endogenous glycopeptides	865:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	5	37	theme	N-linked	865:872	arg1	glycopeptides					898:910	N-linked and O-linked endogenous glycopeptides	865:910	N-linked and O-linked endogenous glycopeptides	865:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	7	38	link	N-linked	1128:1135	arg1	glycopeptide					1150:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	39	link	O-linked	1141:1148	arg1	glycopeptide					1150:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	9	40	theme	biomarker	1498:1506	arg1	discovery					1508:1516	the disease biomarker discovery	1486:1516	the disease biomarker discovery	1486:1516	The developed strategy provides a novel platform for the disease biomarker discovery.					
32090575	7	41	theme	largest	1179:1185	arg1	glycopeptides					1219:1231	endogenously intact glycopeptides	1199:1231	endogenously intact glycopeptides reported so far	1199:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	41	theme	largest	1179:1185	arg1	set					1192:1194	the largest data set	1175:1194	the largest data set of endogenously intact glycopeptides reported so far	1175:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	3	42	gly	glycopeptides	481:493	arg2	glycopeptides					481:493	N-linked and O-linked endogenously intact glycopeptides	439:493	N-linked and O-linked endogenously intact glycopeptides	439:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	6	43	theme	intact	971:976	arg1	O-glycopeptides					978:992	51 intact O-glycopeptides	968:992	51 intact O-glycopeptides	968:992	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	0	44	theme	O-Linked	46:53	arg1	Glycopeptides					55:67	Endogenously Intact N-Linked and O-Linked Glycopeptides	13:67	Endogenously Intact N-Linked and O-Linked Glycopeptides	13:67	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	8	45	theme	cleavage	1311:1318	arg1	sites					1320:1324	cleavage sites	1311:1324	cleavage sites on peptides	1311:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	2	46	theme	glycopeptides	325:337	arg1	identification					287:300	the identification	283:300	the identification of endogenously intact glycopeptides	283:337	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	6	47	theme	sample	1044:1049	arg1	μL					1022:1023	only 40 μL	1014:1023	only 40 μL of the human serum sample	1014:1049	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	4	48	theme	small	726:730	arg1	serum					742:746	serum	742:746	serum	742:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	48	theme	small	726:730	arg1	amount					732:737	a small amount	724:737	a small amount of serum	724:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	7	49	theme	O-linked	1141:1148	arg1	glycopeptide					1150:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	8	50	theme	glycans	1271:1277	arg1	distributions					1254:1266	The distributions	1250:1266	The distributions of glycans among peptides and proteins and cleavage sites on peptides	1250:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	6	51	theme	human	1032:1036	arg1	sample					1044:1049	the human serum sample	1028:1049	the human serum sample	1028:1049	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	7	52	theme	N-linked	1128:1135	arg1	glycopeptide					1150:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	endogenously intact N-linked and O-linked glycopeptide	1108:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	2	53	theme	poor	235:238	arg1	fragmentation					240:252	the poor fragmentation	231:252	the poor fragmentation	231:252	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	4	54	theme	intact	698:703	arg1	glycopeptides					705:717	the endogenously intact glycopeptides	681:717	the endogenously intact glycopeptides from a small amount of serum	681:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	1	55	theme	Endogenous	116:125	arg1	glycopeptides					127:139	Endogenous glycopeptides	116:139	Endogenous glycopeptides in serum	116:148	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	1	55	theme	Endogenous	116:125	arg1	resource					168:175	an invaluable resource	154:175	an invaluable resource for biomarker discovery	154:199	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	1	56	gly	glycopeptides	127:139	arg2	resource					168:175	an invaluable resource	154:175	an invaluable resource for biomarker discovery	154:199	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	1	56	gly	glycopeptides	127:139	arg2	glycopeptides					127:139	Endogenous glycopeptides	116:139	Endogenous glycopeptides in serum	116:148	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	2	57	theme	mass	264:267	arg1	spectrometry					269:280	tandem mass spectrometry	257:280	tandem mass spectrometry	257:280	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	5	58	dep	in	791:792	arg1	silico					794:799	silico	794:799	silico	794:799	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	6	59	gly	N-glycopeptides	948:962	arg2	N-glycopeptides					948:962	223 intact N-glycopeptides	937:962	223 intact N-glycopeptides	937:962	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	0	60	theme	Human	74:78	arg1	Serum					80:84	Human Serum	74:84	Human Serum Using an Integrated Platform	74:113	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	4	61	theme	acid	535:538	arg1	denaturation					540:551	the high-temperature acid denaturation	514:551	the high-temperature acid denaturation	514:551	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	8	62	theme	endogenous	1385:1394	arg1	glycosylation					1396:1408	endogenous glycosylation	1385:1408	endogenous glycosylation	1385:1408	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	1	63	theme	invaluable	157:166	arg1	glycopeptides					127:139	Endogenous glycopeptides	116:139	Endogenous glycopeptides in serum	116:148	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	1	63	theme	invaluable	157:166	arg1	resource					168:175	an invaluable resource	154:175	an invaluable resource for biomarker discovery	154:199	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	6	64	gly	O-glycopeptides	978:992	arg2	O-glycopeptides					978:992	51 intact O-glycopeptides	968:992	51 intact O-glycopeptides	968:992	In total, 223 intact N-glycopeptides and 51 intact O-glycopeptides are identified from only 40 μL of the human serum sample.					
32090575	0	65	theme	Integrated	95:104	arg1	Platform					106:113	an Integrated Platform	92:113	an Integrated Platform	92:113	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	2	66	from	abundance	217:225	arg1	spectrometry					269:280	tandem mass spectrometry	257:280	tandem mass spectrometry	257:280	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	5	67	theme	twin-spectra	767:778	arg1	scheme					780:785	the twin-spectra scheme	763:785	the twin-spectra scheme	763:785	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	4	68	theme	chromatography	599:612	arg1	steps					614:618	chromatography steps	599:618	chromatography steps	599:618	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	0	69	theme	Intact	26:31	arg1	Glycopeptides					55:67	Endogenously Intact N-Linked and O-Linked Glycopeptides	13:67	Endogenously Intact N-Linked and O-Linked Glycopeptides	13:67	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	1	70	from	glycopeptides	127:139	arg1	serum					144:148	serum	144:148	serum	144:148	Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery.					
32090575	2	71	from	fragmentation	240:252	arg1	spectrometry					269:280	tandem mass spectrometry	257:280	tandem mass spectrometry	257:280	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	7	72	theme	glycopeptides	1219:1231	arg1	glycopeptides					1219:1231	endogenously intact glycopeptides	1199:1231	endogenously intact glycopeptides reported so far	1199:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	72	theme	glycopeptides	1219:1231	arg1	study					1070:1074	the first study	1060:1074	the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide	1060:1161	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	72	theme	glycopeptides	1219:1231	arg1	set					1192:1194	the largest data set	1175:1194	the largest data set of endogenously intact glycopeptides reported so far	1175:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	7	72	theme	glycopeptides	1219:1231	arg1	This					1052:1055	This	1052:1055	This	1052:1055	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	9	73	theme	developed	1437:1445	arg1	strategy					1447:1454	The developed strategy	1433:1454	The developed strategy	1433:1454	The developed strategy provides a novel platform for the disease biomarker discovery.					
32090575	4	74	theme	serum	742:746	arg1	serum					742:746	serum	742:746	serum	742:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	74	theme	serum	742:746	arg1	amount					732:737	a small amount	724:737	a small amount of serum	724:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	7	75	gly	glycopeptides	1219:1231	arg2	glycopeptides					1219:1231	endogenously intact glycopeptides	1199:1231	endogenously intact glycopeptides reported so far	1199:1247	This is the first study reporting the identification of endogenously intact N-linked and O-linked glycopeptide and is also the largest data set of endogenously intact glycopeptides reported so far.					
32090575	3	76	theme	glycopeptides	481:493	arg1	identification					421:434	the identification	417:434	the identification of N-linked and O-linked endogenously intact glycopeptides	417:493	Herein, an integrated platform is fabricated for the identification of N-linked and O-linked endogenously intact glycopeptides.					
32090575	0	77	theme	Glycopeptides	55:67	arg1	Profiling					0:8	Profiling	0:8	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.	0:114	Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform.					
32090575	4	78	dep	denaturation	540:551	arg1	steps					614:618	chromatography steps	599:618	chromatography steps	599:618	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	4	79	theme	hydrophilic	575:585	arg1	interaction					587:597	hydrophilic interaction	575:597	hydrophilic interaction	575:597	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	2	80	theme	low	213:215	arg1	abundance					217:225	the low abundance	209:225	the low abundance	209:225	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	2	81	theme	many	351:354	arg1	challenges					356:365	many challenges	351:365	many challenges	351:365	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	9	82	theme	novel	1467:1471	arg1	platform					1473:1480	a novel platform	1465:1480	a novel platform for the disease biomarker discovery	1465:1516	The developed strategy provides a novel platform for the disease biomarker discovery.					
32090575	5	83	link	N-linked	865:872	arg1	glycopeptides					898:910	N-linked and O-linked endogenous glycopeptides	865:910	N-linked and O-linked endogenous glycopeptides	865:910	Additionally, the twin-spectra scheme and in silico deglycosylation strategy were applied for the identification of N-linked and O-linked endogenous glycopeptides, respectively.					
32090575	8	84	from	sites	1320:1324	arg1	peptides					1329:1336	peptides	1329:1336	peptides	1329:1336	The distributions of glycans among peptides and proteins and cleavage sites on peptides are further analyzed to seek the regulation of endogenous glycosylation for disease mechanism.					
32090575	4	85	theme	efficient	657:665	arg1	extraction					667:676	the highly efficient extraction	646:676	the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum	646:746	In this platform, the high-temperature acid denaturation, ultrafiltration, and hydrophilic interaction chromatography steps are combined together for the highly efficient extraction of the endogenously intact glycopeptides from a small amount of serum.					
32090575	2	86	gly	glycopeptides	325:337	arg2	glycopeptides					325:337	endogenously intact glycopeptides	305:337	endogenously intact glycopeptides	305:337	Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenously intact glycopeptides still faces many challenges.					
32090575	9	87	theme	disease	1490:1496	arg1	discovery					1508:1516	the disease biomarker discovery	1486:1516	the disease biomarker discovery	1486:1516	The developed strategy provides a novel platform for the disease biomarker discovery.					
32577656	3	0	theme	other	493:497	arg1	coronaviruses					499:511	other coronaviruses	493:511	other coronaviruses	493:511	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	6	1	from	sites	1243:1247	arg1	N-glycosylation					1211:1225	modeled N-glycosylation	1203:1225	modeled N-glycosylation at known glycan sites	1203:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	0	2	theme	protein	63:69	arg1	structures					71:80	high resolution coronavirus protein structures	35:80	high resolution coronavirus protein structures	35:80	CoV3D: A database and resource for high resolution coronavirus protein structures.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	variability					1020:1030	spike sequence variability	1005:1030	spike sequence variability	1005:1030	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	structures					841:850	structures	841:850	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	841:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	complexes					994:1002	spike-antibody complexes	979:1002	spike-antibody complexes	979:1002	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	polymorphisms					1043:1055	known polymorphisms	1037:1055	known polymorphisms	1037:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	sets					971:974	sets	971:974	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	971:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	3	theme	polymorphisms	1043:1055	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	6	4	theme	structure-based	1074:1088	arg1	design					1090:1095	structure-based design	1074:1095	structure-based design	1074:1095	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	2	5	theme	structures	317:326	arg1	number					291:296	the number	287:296	the number of high resolution structures of SARS-CoV-2 proteins	287:349	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	4	6	with	users	650:654	arg1	sets					675:678	comprehensive sets	661:678	comprehensive sets of structures of coronavirus proteins	661:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	6	with	users	650:654	arg1	molecules					776:784	small molecules	770:784	small molecules	770:784	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	6	with	users	650:654	arg1	receptors					755:763	receptors	755:763	receptors	755:763	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	6	with	users	650:654	arg1	structures					683:692	structures	683:692	structures of coronavirus proteins	683:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	6	with	users	650:654	arg1	antibodies					743:752	antibodies	743:752	antibodies	743:752	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	5	7	theme	spike-antibody	979:992	arg1	complexes					994:1002	spike-antibody complexes	979:1002	spike-antibody complexes	979:1002	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	8	gly	glycoprotein	865:876	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	8	gly	glycoprotein	865:876	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	9	theme	spike	1005:1009	arg1	variability					1020:1030	spike sequence variability	1005:1030	spike sequence variability	1005:1030	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	10	theme	efforts	951:957	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	10	theme	efforts	951:957	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	11	theme	design	944:949	arg1	efforts					951:957	vaccine design efforts	936:957	vaccine design efforts	936:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	1	12	theme	general	148:154	arg1	threat					156:161	the general threat	144:161	the general threat to global health posed by coronaviruses	144:201	SARS-CoV-2, the etiologic agent behind COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32577656	7	13	theme	other	1449:1453	arg1	structures					1475:1484	other coronavirus protein structures	1449:1484	other coronavirus protein structures	1449:1484	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	6	14	contain	contains	1254:1261	arg2	classification					1279:1292	structure-based classification	1263:1292	structure-based classification of spike conformations, generated by unsupervised clustering	1263:1353	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	14	contain	contains	1254:1261	arg1	CoV3D					1137:1141	CoV3D	1137:1141	CoV3D	1137:1141	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	4	15	theme	coronavirus	697:707	arg1	proteins					709:716	coronavirus proteins	697:716	coronavirus proteins	697:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	2	16	theme	resolution	306:315	arg1	structures					317:326	high resolution structures	301:326	high resolution structures of SARS-CoV-2 proteins	301:349	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	5	17	theme	spike	859:863	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	17	theme	spike	859:863	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	6	18	gly	N-glycosylation	1211:1225	arg1	structures					1187:1196	spike structures	1181:1196	spike structures with modeled N-glycosylation at known glycan sites	1181:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	18	gly	N-glycosylation	1211:1225	arg1	sites					1243:1247	known glycan sites	1230:1247	known glycan sites	1230:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	18	gly	N-glycosylation	1211:1225	arg2	sites					1243:1247	known glycan sites	1230:1247	known glycan sites	1230:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	7	19	theme	coronavirus	1455:1465	arg1	structures					1475:1484	other coronavirus protein structures	1449:1484	other coronavirus protein structures	1449:1484	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	3	20	theme	structure-based	418:432	arg1	design					434:439	structure-based design	418:439	structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses	418:511	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	5	21	theme	Integrated	787:796	arg1	viewers					808:814	Integrated molecular viewers	787:814	Integrated molecular viewers	787:814	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	7	22	theme	protein	1467:1473	arg1	structures					1475:1484	other coronavirus protein structures	1449:1484	other coronavirus protein structures	1449:1484	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	5	23	theme	antibodies	921:930	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	23	theme	antibodies	921:930	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	1	24	theme	global	166:171	arg1	health					173:178	global health	166:178	global health posed by coronaviruses	166:201	SARS-CoV-2, the etiologic agent behind COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32577656	5	25	theme	molecular	798:806	arg1	viewers					808:814	Integrated molecular viewers	787:814	Integrated molecular viewers	787:814	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	6	26	with	structures	1187:1196	arg1	N-glycosylation					1211:1225	modeled N-glycosylation	1203:1225	modeled N-glycosylation at known glycan sites	1203:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	27	theme	glycan	1236:1241	arg1	sites					1243:1247	known glycan sites	1230:1247	known glycan sites	1230:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	5	28	theme	variability	1020:1030	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	28	theme	variability	1020:1030	arg1	variability					1020:1030	spike sequence variability	1005:1030	spike sequence variability	1005:1030	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	28	theme	variability	1020:1030	arg1	structures					841:850	structures	841:850	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	841:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	28	theme	variability	1020:1030	arg1	complexes					994:1002	spike-antibody complexes	979:1002	spike-antibody complexes	979:1002	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	28	theme	variability	1020:1030	arg1	polymorphisms					1043:1055	known polymorphisms	1037:1055	known polymorphisms	1037:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	28	theme	variability	1020:1030	arg1	sets					971:974	sets	971:974	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	971:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	28	theme	variability	1020:1030	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	variability					1020:1030	spike sequence variability	1005:1030	spike sequence variability	1005:1030	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	structures					841:850	structures	841:850	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	841:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	complexes					994:1002	spike-antibody complexes	979:1002	spike-antibody complexes	979:1002	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	polymorphisms					1043:1055	known polymorphisms	1037:1055	known polymorphisms	1037:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	sets					971:974	sets	971:974	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	971:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	29	theme	glycoprotein	865:876	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	7	30	theme	research	1376:1383	arg1	community					1385:1393	the research community	1372:1393	the research community	1372:1393	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	7	30	theme	research	1376:1383	arg1	CoV3D					1356:1360	CoV3D	1356:1360	CoV3D	1356:1360	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	7	30	theme	research	1376:1383	arg1	reference					1412:1420	a centralized reference	1398:1420	a centralized reference	1398:1420	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	5	31	theme	neutralizing	908:919	arg1	antibodies					921:930	neutralizing antibodies	908:930	neutralizing antibodies	908:930	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	32	theme	vaccine	936:942	arg1	efforts					951:957	vaccine design efforts	936:957	vaccine design efforts	936:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	variability					1020:1030	spike sequence variability	1005:1030	spike sequence variability	1005:1030	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	structures					841:850	structures	841:850	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	841:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	complexes					994:1002	spike-antibody complexes	979:1002	spike-antibody complexes	979:1002	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	polymorphisms					1043:1055	known polymorphisms	1037:1055	known polymorphisms	1037:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	sets					971:974	sets	971:974	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	971:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	33	theme	complexes	994:1002	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	3	34	theme	vaccines	444:451	arg1	design					434:439	structure-based design	418:439	structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses	418:511	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	6	35	gly	glycoprotein	1123:1134	arg1	glycoprotein					1123:1134	the spike glycoprotein	1113:1134	the spike glycoprotein	1113:1134	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	3	36	theme	weekly	621:626	arg1	basis					628:632	a weekly basis	619:632	a weekly basis	619:632	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	2	37	theme	rapid	273:277	arg1	rise					279:282	a rapid rise	271:282	a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities	271:405	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	2	38	theme	virus	385:389	arg1	vulnerabilities					391:405	virus vulnerabilities	385:405	virus vulnerabilities	385:405	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	4	39	theme	structures	683:692	arg1	sets					675:678	comprehensive sets	661:678	comprehensive sets of structures of coronavirus proteins	661:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	39	theme	structures	683:692	arg1	structures					683:692	structures	683:692	structures of coronavirus proteins	683:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	6	40	theme	unsupervised	1331:1342	arg1	clustering					1344:1353	unsupervised clustering	1331:1353	unsupervised clustering	1331:1353	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	7	41	dep	serve	1366:1370	arg1	//cov3d.ibbr.umd.edu					1514:1533	//cov3d.ibbr.umd.edu	1514:1533	//cov3d.ibbr.umd.edu	1514:1533	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	7	41	dep	serve	1366:1370	arg1	https					1508:1512	https	1508:1512	https	1508:1512	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	3	42	theme	protein	579:585	arg1	structures					587:596	coronavirus protein structures	567:596	coronavirus protein structures	567:596	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	6	43	theme	structure-based	1263:1277	arg1	classification					1279:1292	structure-based classification	1263:1292	structure-based classification of spike conformations, generated by unsupervised clustering	1263:1353	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	44	theme	known	1230:1234	arg1	sites					1243:1247	known glycan sites	1230:1247	known glycan sites	1230:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	4	45	theme	comprehensive	661:673	arg1	sets					675:678	comprehensive sets	661:678	comprehensive sets of structures of coronavirus proteins	661:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	45	theme	comprehensive	661:673	arg1	structures					683:692	structures	683:692	structures of coronavirus proteins	683:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	7	46	theme	centralized	1400:1410	arg1	community					1385:1393	the research community	1372:1393	the research community	1372:1393	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	7	46	theme	centralized	1400:1410	arg1	CoV3D					1356:1360	CoV3D	1356:1360	CoV3D	1356:1360	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	7	46	theme	centralized	1400:1410	arg1	reference					1412:1420	a centralized reference	1398:1420	a centralized reference	1398:1420	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32577656	0	47	theme	high	35:38	arg1	structures					71:80	high resolution coronavirus protein structures	35:80	high resolution coronavirus protein structures	35:80	CoV3D: A database and resource for high resolution coronavirus protein structures.					
32577656	4	48	with	complexes	728:736	arg1	molecules					776:784	small molecules	770:784	small molecules	770:784	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	48	with	complexes	728:736	arg1	sets					675:678	comprehensive sets	661:678	comprehensive sets of structures of coronavirus proteins	661:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	48	with	complexes	728:736	arg1	receptors					755:763	receptors	755:763	receptors	755:763	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	48	with	complexes	728:736	arg1	structures					683:692	structures	683:692	structures of coronavirus proteins	683:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	4	48	with	complexes	728:736	arg1	antibodies					743:752	antibodies	743:752	antibodies	743:752	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	2	49	theme	urgent	208:213	arg1	need					215:218	The urgent need	204:218	The urgent need for effective vaccines and therapies	204:255	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	1	50	theme	etiologic	99:107	arg1	SARS-CoV-2					83:92	SARS-CoV-2	83:92	SARS-CoV-2	83:92	SARS-CoV-2, the etiologic agent behind COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32577656	1	50	theme	etiologic	99:107	arg1	agent					109:113	the etiologic agent	95:113	the etiologic agent behind COVID-19	95:129	SARS-CoV-2, the etiologic agent behind COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32577656	5	51	theme	sequence	1011:1018	arg1	variability					1020:1030	spike sequence variability	1005:1030	spike sequence variability	1005:1030	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	3	52	theme	coronavirus	567:577	arg1	structures					587:596	coronavirus protein structures	567:596	coronavirus protein structures	567:596	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	2	53	theme	high	301:304	arg1	structures					317:326	high resolution structures	301:326	high resolution structures of SARS-CoV-2 proteins	301:349	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	6	54	theme	modeled	1203:1209	arg1	N-glycosylation					1211:1225	modeled N-glycosylation	1203:1225	modeled N-glycosylation at known glycan sites	1203:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	0	55	dep	CoV3D	0:4	arg1	resource					22:29	resource	22:29	resource	22:29	CoV3D: A database and resource for high resolution coronavirus protein structures.					
32577656	0	55	dep	CoV3D	0:4	arg1	database					9:16	A database	7:16	A database	7:16	CoV3D: A database and resource for high resolution coronavirus protein structures.					
32577656	6	56	theme	conformations	1303:1315	arg1	classification					1279:1292	structure-based classification	1263:1292	structure-based classification of spike conformations, generated by unsupervised clustering	1263:1353	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	4	57	theme	small	770:774	arg1	molecules					776:784	small molecules	770:784	small molecules	770:784	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	3	58	theme	therapeutics	457:468	arg1	design					434:439	structure-based design	418:439	structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses	418:511	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	6	59	theme	glycoprotein	1123:1134	arg1	analysis					1101:1108	analysis	1101:1108	analysis of the spike glycoprotein	1101:1134	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	59	theme	glycoprotein	1123:1134	arg1	design					1090:1095	structure-based design	1074:1095	structure-based design	1074:1095	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	60	theme	spike	1297:1301	arg1	conformations					1303:1315	spike conformations	1297:1315	spike conformations	1297:1315	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	5	61	theme	major	892:896	arg1	target					898:903	the major target	888:903	the major target of neutralizing antibodies and vaccine design efforts	888:957	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	5	61	theme	major	892:896	arg1	glycoprotein					865:876	the spike glycoprotein	855:876	the spike glycoprotein	855:876	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	6	62	theme	structures	1187:1196	arg1	visualization					1151:1163	visualization	1151:1163	visualization	1151:1163	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	6	62	theme	structures	1187:1196	arg1	download					1169:1176	download	1169:1176	download	1169:1176	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	3	63	dep	database	541:548	arg1	a					539:539	a	539:539	a	539:539	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32577656	4	64	theme	proteins	709:716	arg1	structures					683:692	structures	683:692	structures of coronavirus proteins	683:716	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32577656	0	65	theme	coronavirus	51:61	arg1	structures					71:80	high resolution coronavirus protein structures	35:80	high resolution coronavirus protein structures	35:80	CoV3D: A database and resource for high resolution coronavirus protein structures.					
32577656	5	66	theme	known	1037:1041	arg1	polymorphisms					1043:1055	known polymorphisms	1037:1055	known polymorphisms	1037:1055	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32577656	6	67	theme	spike	1181:1185	arg1	structures					1187:1196	spike structures	1181:1196	spike structures with modeled N-glycosylation at known glycan sites	1181:1247	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	2	68	theme	vulnerabilities	391:405	arg1	map					378:380	a map	376:380	a map of virus vulnerabilities	376:405	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	2	69	theme	proteins	342:349	arg1	structures					317:326	high resolution structures	301:326	high resolution structures of SARS-CoV-2 proteins	301:349	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	0	70	theme	resolution	40:49	arg1	structures					71:80	high resolution coronavirus protein structures	35:80	high resolution coronavirus protein structures	35:80	CoV3D: A database and resource for high resolution coronavirus protein structures.					
32577656	6	71	theme	spike	1117:1121	arg1	glycoprotein					1123:1134	the spike glycoprotein	1113:1134	the spike glycoprotein	1113:1134	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32577656	2	72	theme	effective	224:232	arg1	vaccines					234:241	effective vaccines	224:241	effective vaccines	224:241	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	2	73	theme	SARS-CoV-2	331:340	arg1	proteins					342:349	SARS-CoV-2 proteins	331:349	SARS-CoV-2 proteins	331:349	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32577656	2	74	from	rise	279:282	arg1	number					291:296	the number	287:296	the number of high resolution structures of SARS-CoV-2 proteins	287:349	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32544582	4	0	theme	A	454:454	arg1	loading					456:462	The Callophycin A loading	438:462	The Callophycin A loading	438:462	The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM.					
32544582	5	1	theme	Cal	577:579	arg1	A					581:581	Cal A	577:581	Cal A	577:581	Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively.					
32544582	9	2	theme	first	1036:1040	arg1	study					1055:1059	the first and foremost study	1032:1059	the first and foremost study to discriminate the potentiality of Cal A composites	1032:1112	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	9	2	theme	first	1036:1040	arg1	investigations					1001:1014	The present investigations	989:1014	The present investigations	989:1014	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	2	3	theme	albicans	322:329	arg1	viability					306:314	the viability	302:314	the viability of C. albicans	302:329	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	2	4	from	effects	282:288	arg1	viability					306:314	the viability	302:314	the viability of C. albicans	302:329	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	0	5	theme	alternative	70:80	arg1	strategy					82:89	an alternative strategy	67:89	an alternative strategy to overcome vaginal candidiasis	67:121	Callophycin A loaded chitosan and spicules based nanocomposites as an alternative strategy to overcome vaginal candidiasis.					
32544582	5	6	theme	A	581:581	arg1	%					572:572	38%	570:572	38%	570:572	Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively.					
32544582	5	6	theme	A	581:581	arg1	A					581:581	Cal A	577:581	Cal A	577:581	Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively.					
32544582	5	6	theme	A	581:581	arg1	%					564:564	65%	562:564	65%	562:564	Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively.					
32544582	2	7	theme	C.	319:320	arg1	albicans					322:329	C. albicans	319:329	C. albicans	319:329	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	9	8	theme	Cal	1097:1099	arg1	composites					1103:1112	Cal A composites	1097:1112	Cal A composites	1097:1112	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	10	9	theme	loaded	1121:1126	arg1	chitosan					1128:1135	A loaded chitosan	1119:1135	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	1	10	link	derived	193:199	arg1	A					231:231	Cal A	227:231	Cal A	227:231	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	1	10	link	derived	193:199	arg1	A					224:224	seaweed derived metabolite Callophycin A	185:224	seaweed derived metabolite Callophycin A (Cal A)	185:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	5	11	theme	around	555:560	arg1	A					581:581	Cal A	577:581	Cal A	577:581	Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively.					
32544582	5	11	theme	around	555:560	arg1	%					564:564	65%	562:564	65%	562:564	Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively.					
32544582	2	12	theme	beneficial	271:280	arg1	effects					282:288	the beneficial effects	267:288	the beneficial effects of Cal A on the viability of C. albicans	267:329	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	6	13	dep	confirmed	692:700	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	13	dep	confirmed	692:700	arg1	Chi					764:766	Cal@Chi	760:766	Cal@Chi	760:766	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	13	dep	confirmed	692:700	arg1	A					757:757	Cal A	753:757	Cal A	753:757	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	13	dep	confirmed	692:700	arg1	Spi					776:778	Cal@Spi	772:778	Cal@Spi	772:778	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	8	14	dep	evidenced	929:937	arg1	that					939:942	that	939:942	that	939:942	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	6	15	theme	%	737:737	arg1	clotrimazole					739:750	1% clotrimazole	736:750	1% clotrimazole	736:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	8	16	theme	protective	948:957	arg1	role					959:962	the protective role	944:962	the protective role of Cal@Chi in VVC model	944:986	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	3	17	theme	drug	424:427	arg1	carrier					429:435	a drug carrier	422:435	a drug carrier	422:435	To enhance the biological activity, we used to demonstrated that chitosan and spicules as a drug carrier.					
32544582	10	18	theme	marine	1225:1230	arg1	product					1240:1246	the novel marine natural product	1215:1246	the novel marine natural product based topical applications	1215:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	10	19	theme	novel	1219:1223	arg1	product					1240:1246	the novel marine natural product	1215:1246	the novel marine natural product based topical applications	1215:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	1	20	theme	killing	166:172	arg1	effects					174:180	the killing effects	162:180	the killing effects of seaweed derived metabolite Callophycin A (Cal A)	162:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	0	21	theme	vaginal	103:109	arg1	candidiasis					111:121	vaginal candidiasis	103:121	vaginal candidiasis	103:121	Callophycin A loaded chitosan and spicules based nanocomposites as an alternative strategy to overcome vaginal candidiasis.					
32544582	4	22	theme	Callophycin	442:452	arg1	loading					456:462	The Callophycin A loading	438:462	The Callophycin A loading	438:462	The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM.					
32544582	3	23	theme	biological	347:356	arg1	activity					358:365	the biological activity	343:365	the biological activity	343:365	To enhance the biological activity, we used to demonstrated that chitosan and spicules as a drug carrier.					
32544582	6	24	theme	@	775:775	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	24	theme	@	775:775	arg1	Spi					776:778	Cal@Spi	772:778	Cal@Spi	772:778	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	8	25	theme	histo-morphological	892:910	arg1	alterations					912:922	The histo-morphological alterations	888:922	The histo-morphological alterations	888:922	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	2	26	theme	A	297:297	arg1	effects					282:288	the beneficial effects	267:288	the beneficial effects of Cal A on the viability of C. albicans	267:329	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	0	27	theme	Callophycin	0:10	arg1	A					12:12	Callophycin A	0:12	Callophycin A	0:12	Callophycin A loaded chitosan and spicules based nanocomposites as an alternative strategy to overcome vaginal candidiasis.					
32544582	9	28	theme	foremost	1046:1053	arg1	study					1055:1059	the first and foremost study	1032:1059	the first and foremost study to discriminate the potentiality of Cal A composites	1032:1112	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	9	28	theme	foremost	1046:1053	arg1	investigations					1001:1014	The present investigations	989:1014	The present investigations	989:1014	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	6	29	theme	candidicidal	711:722	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	29	theme	candidicidal	711:722	arg1	Chi					764:766	Cal@Chi	760:766	Cal@Chi	760:766	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	29	theme	candidicidal	711:722	arg1	A					757:757	Cal A	753:757	Cal A	753:757	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	29	theme	candidicidal	711:722	arg1	Spi					776:778	Cal@Spi	772:778	Cal@Spi	772:778	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	2	30	theme	Cal	293:295	arg1	A					297:297	Cal A	293:297	Cal A	293:297	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	1	31	theme	seaweed	185:191	arg1	A					231:231	Cal A	227:231	Cal A	227:231	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	1	31	theme	seaweed	185:191	arg1	A					224:224	seaweed derived metabolite Callophycin A	185:224	seaweed derived metabolite Callophycin A (Cal A)	185:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	4	32	theme	FT-IR	503:507	arg1	variation					490:498	spectral variation	481:498	spectral variation of FT-IR	481:507	The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM.					
32544582	4	32	theme	FT-IR	503:507	arg1	variation					527:535	morphological variation	513:535	morphological variation by SEM	513:542	The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM.					
32544582	8	33	theme	@	970:970	arg1	Chi					971:973	Cal@Chi	967:973	Cal@Chi	967:973	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	6	34	theme	1	736:736	arg1	%					737:737	%	737:737	%	737:737	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	35	theme	clotrimazole	739:750	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	35	theme	clotrimazole	739:750	arg1	Chi					764:766	Cal@Chi	760:766	Cal@Chi	760:766	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	35	theme	clotrimazole	739:750	arg1	A					757:757	Cal A	753:757	Cal A	753:757	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	35	theme	clotrimazole	739:750	arg1	Spi					776:778	Cal@Spi	772:778	Cal@Spi	772:778	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	10	36	theme	alternative	1171:1181	arg1	nanoparticles					1137:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	10	36	theme	alternative	1171:1181	arg1	strategy					1183:1190	an alternative strategy	1168:1190	an alternative strategy for the development of the novel marine natural product based topical applications	1168:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	1	37	theme	derived	193:199	arg1	A					231:231	Cal A	227:231	Cal A	227:231	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	1	37	theme	derived	193:199	arg1	A					224:224	seaweed derived metabolite Callophycin A	185:224	seaweed derived metabolite Callophycin A (Cal A)	185:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	7	38	theme	vaginal	872:878	arg1	lavage					880:885	vaginal lavage	872:885	vaginal lavage	872:885	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	8	39	theme	Cal	967:969	arg1	Chi					971:973	Cal@Chi	967:973	Cal@Chi	967:973	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	6	40	theme	Cal	772:774	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	40	theme	Cal	772:774	arg1	Spi					776:778	Cal@Spi	772:778	Cal@Spi	772:778	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	1	41	theme	metabolite	201:210	arg1	A					231:231	Cal A	227:231	Cal A	227:231	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	1	41	theme	metabolite	201:210	arg1	A					224:224	seaweed derived metabolite Callophycin A	185:224	seaweed derived metabolite Callophycin A (Cal A)	185:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	4	42	theme	morphological	513:525	arg1	variation					527:535	morphological variation	513:535	morphological variation by SEM	513:542	The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM.					
32544582	9	43	theme	A	1101:1101	arg1	composites					1103:1112	Cal A composites	1097:1112	Cal A composites	1097:1112	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	1	44	theme	Callophycin	212:222	arg1	A					231:231	Cal A	227:231	Cal A	227:231	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	1	44	theme	Callophycin	212:222	arg1	A					224:224	seaweed derived metabolite Callophycin A	185:224	seaweed derived metabolite Callophycin A (Cal A)	185:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	2	45	dep	In	235:236	arg1	vitro					238:242	vitro	238:242	vitro	238:242	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	4	46	theme	spectral	481:488	arg1	variation					490:498	spectral variation	481:498	spectral variation of FT-IR	481:507	The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM.					
32544582	8	47	theme	Chi	971:973	arg1	role					959:962	the protective role	944:962	the protective role of Cal@Chi in VVC model	944:986	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	9	48	theme	composites	1103:1112	arg1	potentiality					1081:1092	the potentiality	1077:1092	the potentiality of Cal A composites	1077:1112	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	9	49	theme	present	993:999	arg1	study					1055:1059	the first and foremost study	1032:1059	the first and foremost study to discriminate the potentiality of Cal A composites	1032:1112	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	9	49	theme	present	993:999	arg1	investigations					1001:1014	The present investigations	989:1014	The present investigations	989:1014	The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites.					
32544582	1	50	theme	A	224:224	arg1	effects					174:180	the killing effects	162:180	the killing effects of seaweed derived metabolite Callophycin A (Cal A)	162:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	8	51	from	role	959:962	arg1	model					982:986	VVC model	978:986	VVC model	978:986	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	6	52	theme	@	763:763	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	52	theme	@	763:763	arg1	Chi					764:766	Cal@Chi	760:766	Cal@Chi	760:766	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	10	53	theme	natural	1232:1238	arg1	product					1240:1246	the novel marine natural product	1215:1246	the novel marine natural product based topical applications	1215:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	7	54	theme	significant	826:836	arg1	reduction					838:846	a significant reduction	824:846	a significant reduction in the fungal burden of vaginal lavage	824:885	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	0	55	theme	based	43:47	arg1	nanocomposites					49:62	based nanocomposites	43:62	based nanocomposites	43:62	Callophycin A loaded chitosan and spicules based nanocomposites as an alternative strategy to overcome vaginal candidiasis.					
32544582	10	56	theme	topical	1254:1260	arg1	applications					1262:1273	topical applications	1254:1273	topical applications	1254:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	8	57	theme	VVC	978:980	arg1	model					982:986	VVC model	978:986	VVC model	978:986	The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model.					
32544582	6	58	theme	Cal	760:762	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	58	theme	Cal	760:762	arg1	Chi					764:766	Cal@Chi	760:766	Cal@Chi	760:766	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	7	59	theme	lavage	880:885	arg1	burden					862:867	the fungal burden	851:867	the fungal burden of vaginal lavage	851:885	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	7	60	theme	treatment	797:805	arg1	6 days					787:792	6 days	787:792	6 days of treatment	787:805	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	10	61	used	used	1160:1163	arg2	strategy					1183:1190	an alternative strategy	1168:1190	an alternative strategy for the development of the novel marine natural product based topical applications	1168:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	10	61	used	used	1160:1163	arg2	nanoparticles					1137:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	0	62	dep	chitosan	21:28	arg1	nanocomposites					49:62	based nanocomposites	43:62	based nanocomposites	43:62	Callophycin A loaded chitosan and spicules based nanocomposites as an alternative strategy to overcome vaginal candidiasis.					
32544582	6	63	theme	model	674:678	arg1	experiments					680:690	VVC induced animal model experiments	655:690	VVC induced animal model experiments	655:690	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	1	64	theme	present	128:134	arg1	study					136:140	The present study	124:140	The present study	124:140	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	7	65	theme	Cal	807:809	arg1	Chi					811:813	Cal@Chi	807:813	Cal@Chi	807:813	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	3	66	used	used	371:374	arg2	we					368:369	we	368:369	we	368:369	To enhance the biological activity, we used to demonstrated that chitosan and spicules as a drug carrier.					
32544582	10	67	theme	Cal	1115:1117	arg1	nanoparticles					1137:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	10	67	theme	Cal	1115:1117	arg1	strategy					1183:1190	an alternative strategy	1168:1190	an alternative strategy for the development of the novel marine natural product based topical applications	1168:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	6	68	theme	animal	667:672	arg1	experiments					680:690	VVC induced animal model experiments	655:690	VVC induced animal model experiments	655:690	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	7	69	from	reduction	838:846	arg1	burden					862:867	the fungal burden	851:867	the fungal burden of vaginal lavage	851:885	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	1	70	theme	Cal	227:229	arg1	A					231:231	Cal A	227:231	Cal A	227:231	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	1	70	theme	Cal	227:229	arg1	A					224:224	seaweed derived metabolite Callophycin A	185:224	seaweed derived metabolite Callophycin A (Cal A)	185:232	The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A).					
32544582	6	71	theme	Cal	753:755	arg1	activity					724:731	the candidicidal activity	707:731	the candidicidal activity of 1% clotrimazole	707:750	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	71	theme	Cal	753:755	arg1	A					757:757	Cal A	753:757	Cal A	753:757	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	2	72	theme	In	235:236	arg1	studies					244:250	In vitro studies	235:250	In vitro studies	235:250	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	6	73	theme	induced	659:665	arg1	experiments					680:690	VVC induced animal model experiments	655:690	VVC induced animal model experiments	655:690	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	6	74	dep	induced	659:665	arg1	VVC					655:657	VVC	655:657	VVC	655:657	Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi.					
32544582	10	75	theme	product	1240:1246	arg1	development					1200:1210	the development	1196:1210	the development of the novel marine natural product based topical applications	1196:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	7	76	theme	fungal	855:860	arg1	burden					862:867	the fungal burden	851:867	the fungal burden of vaginal lavage	851:885	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
32544582	2	77	dep	confirmed	252:260	arg1	effects					282:288	the beneficial effects	267:288	the beneficial effects of Cal A on the viability of C. albicans	267:329	In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans.					
32544582	10	78	theme	chitosan	1128:1135	arg1	nanoparticles					1137:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles	1115:1149	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	10	78	theme	chitosan	1128:1135	arg1	strategy					1183:1190	an alternative strategy	1168:1190	an alternative strategy for the development of the novel marine natural product based topical applications	1168:1273	Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications.					
32544582	7	79	theme	@	810:810	arg1	Chi					811:813	Cal@Chi	807:813	Cal@Chi	807:813	After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage.					
34489028	9	0	theme	μA	1519:1520	arg1	limit					1455:1459	The sensitivity and detection limit	1425:1459	limit	1455:1459	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	9	0	theme	μA	1519:1520	arg1	cm-2					1530:1533	466.7 μA mol-1 L cm-2	1513:1533	466.7 μA mol-1 L cm-2	1513:1533	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	3	1	from	immobilization	587:600	arg1	electrode					645:653	a cellulose modified electrode	624:653	a cellulose modified electrode	624:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	9	2	theme	L	1528:1528	arg1	limit					1455:1459	The sensitivity and detection limit	1425:1459	limit	1455:1459	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	9	2	theme	L	1528:1528	arg1	cm-2					1530:1533	466.7 μA mol-1 L cm-2	1513:1533	466.7 μA mol-1 L cm-2	1513:1533	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	3	3	theme	modified	636:643	arg1	electrode					645:653	a cellulose modified electrode	624:653	a cellulose modified electrode	624:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	10	4	theme	glucose	1587:1593	arg1	biosensor					1595:1603	the glucose biosensor	1583:1603	the glucose biosensor	1583:1603	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	11	5	theme	performance	1929:1939	arg1	biosensor					1949:1957	a high performance glucose biosensor	1922:1957	a high performance glucose biosensor	1922:1957	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	10	6	theme	rapid	1617:1621	arg1	change					1631:1636	a rapid current change	1615:1636	a rapid current change (< 5 s)	1615:1644	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	6	theme	rapid	1617:1621	arg1	s					1643:1643	< 5 s	1639:1643	< 5 s	1639:1643	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	1	7	theme	immobilization	172:185	arg1	important					240:248	important	240:248	important	240:248	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	1	7	theme	immobilization	172:185	arg1	method					162:167	The method	158:167	The method of immobilization of glucose oxidase (GOD) on electrodes	158:224	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	11	8	theme	immobilization	1849:1862	arg1	strategy					1864:1871	The affinity adsorption-based immobilization strategy	1819:1871	The affinity adsorption-based immobilization strategy for GOD	1819:1879	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	9	9	theme	sensitivity	1429:1439	arg1	limit					1455:1459	The sensitivity and detection limit	1425:1459	limit	1455:1459	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	9	9	theme	sensitivity	1429:1439	arg1	cm-2					1530:1533	466.7 μA mol-1 L cm-2	1513:1533	466.7 μA mol-1 L cm-2	1513:1533	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	1	10	theme	glucose	190:196	arg1	GOD					207:209	GOD	207:209	GOD	207:209	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	1	10	theme	glucose	190:196	arg1	oxidase					198:204	glucose oxidase	190:204	glucose oxidase (GOD)	190:210	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	11	11	theme	affinity	1823:1830	arg1	strategy					1864:1871	The affinity adsorption-based immobilization strategy	1819:1871	The affinity adsorption-based immobilization strategy for GOD	1819:1879	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	8	12	theme	biosensor	1334:1342	arg1	response					1318:1325	the current signal response	1299:1325	the current signal response of the biosensor to glucose	1299:1353	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	12	theme	biosensor	1334:1342	arg1	linear					1359:1364	linear	1359:1364	linear	1359:1364	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	12	theme	biosensor	1334:1342	arg1	r2					1413:1414	r2 ≥ 0.99	1413:1421	r2 ≥ 0.99	1413:1421	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	10	13	theme	<	1639:1639	arg1	change					1631:1636	a rapid current change	1615:1636	a rapid current change (< 5 s)	1615:1644	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	13	theme	<	1639:1639	arg1	s					1643:1643	< 5 s	1639:1643	< 5 s	1639:1643	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	8	14	theme	≥	1416:1416	arg1	response					1318:1325	the current signal response	1299:1325	the current signal response of the biosensor to glucose	1299:1353	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	14	theme	≥	1416:1416	arg1	linear					1359:1364	linear	1359:1364	linear	1359:1364	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	14	theme	≥	1416:1416	arg1	r2					1413:1414	r2 ≥ 0.99	1413:1421	r2 ≥ 0.99	1413:1421	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	1	15	from	electrodes	215:224	arg1	important					240:248	important	240:248	important	240:248	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	1	15	from	electrodes	215:224	arg1	method					162:167	The method	158:167	The method of immobilization of glucose oxidase (GOD) on electrodes	158:224	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	5	16	theme	binding	808:814	arg1	capacity					816:823	its highest binding capacity	796:823	its highest binding capacity	796:823	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	2	17	theme	fusion	496:501	arg1	GOD-NL-CBM2					508:518	GOD-NL-CBM2	508:518	GOD-NL-CBM2	508:518	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	2	17	theme	fusion	496:501	arg1	GOD					503:505	a fusion GOD	494:505	a fusion GOD (GOD-NL-CBM2)	494:519	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	7	18	from	peak	1129:1132	arg1	presence					1159:1166	the presence	1155:1166	the presence of glucose	1155:1177	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	6	19	theme	composition	896:906	arg1	analysis					908:915	The morphology and composition analysis	877:915	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2	877:981	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2 confirmed the immobilization of GOD-NL-CBM2.					
34489028	5	20	dep	conditions	849:858	arg1	°C					872:873	50 °C	869:873	50 °C	869:873	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	5	20	dep	conditions	849:858	arg1	pH					861:862	pH 5.0	861:866	pH 5.0	861:866	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	10	21	theme	current	1786:1792	arg1	response					1794:1801	the original current response	1773:1801	the original current response	1773:1801	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	7	22	theme	H2O2	1137:1140	arg1	peak					1129:1132	a characteristic peak	1112:1132	a characteristic peak of H2O2 at +0.6 V in the presence of glucose	1112:1177	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	6	23	theme	morphology	881:890	arg1	analysis					908:915	The morphology and composition analysis	877:915	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2	877:981	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2 confirmed the immobilization of GOD-NL-CBM2.					
34489028	0	24	theme	carbohydrate	56:67	arg1	family					84:89	a carbohydrate binding module family	54:89	a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode	54:155	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	1	25	dep	fabrication	258:268	arg1	the					254:256	the	254:256	the	254:256	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	4	26	theme	GOD-NL-CBM2	681:691	arg1	activity					669:676	The specific activity	656:676	The specific activity of GOD-NL-CBM2	656:691	The specific activity of GOD-NL-CBM2 was comparable to that of the wild type GOD.					
34489028	4	26	theme	GOD-NL-CBM2	681:691	arg1	comparable					697:706	comparable	697:706	comparable	697:706	The specific activity of GOD-NL-CBM2 was comparable to that of the wild type GOD.					
34489028	0	27	theme	module	77:82	arg1	family					84:89	a carbohydrate binding module family	54:89	a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode	54:155	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	6	28	dep	morphology	881:890	arg1	The					877:879	The	877:879	The	877:879	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2 confirmed the immobilization of GOD-NL-CBM2.					
34489028	8	29	from	linear	1359:1364	arg1	range					1387:1391	the concentration range	1369:1391	the concentration range from 1.25 to 40 mM	1369:1410	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	0	30	theme	glucose	29:35	arg1	oxidase					37:43	glucose oxidase	29:43	glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode	29:155	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	7	31	theme	GOD-NL-CBM2	1223:1233	arg1	capability					1193:1202	the capability	1189:1202	the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2	1189:1282	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	7	32	theme	film	1088:1091	arg1	bioelectrode					1093:1104	the GOD-NL-CBM2/cellulose film bioelectrode	1062:1104	the GOD-NL-CBM2/cellulose film bioelectrode	1062:1104	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	5	33	theme	catalytic	839:847	arg1	conditions					849:858	optimal catalytic conditions	831:858	optimal catalytic conditions (pH 5.0, 50 °C)	831:874	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	10	34	theme	Relative	1669:1676	arg1	%					1705:1705	RSD < 5%	1698:1705	RSD < 5%	1698:1705	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	34	theme	Relative	1669:1676	arg1	deviation					1687:1695	Relative standard deviation	1669:1695	Relative standard deviation	1669:1695	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	3	35	used	used	548:551	arg2	tag					579:581	an affinity adsorption tag	556:581	an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode	556:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	3	35	used	used	548:551	arg2	CBM2					539:542	The CBM2	535:542	The CBM2	535:542	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	8	36	from	range	1387:1391	arg1	response					1318:1325	the current signal response	1299:1325	the current signal response of the biosensor to glucose	1299:1353	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	36	from	range	1387:1391	arg1	linear					1359:1364	linear	1359:1364	linear	1359:1364	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	36	from	range	1387:1391	arg1	r2					1413:1414	r2 ≥ 0.99	1413:1421	r2 ≥ 0.99	1413:1421	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	9	37	theme	detection	1445:1453	arg1	limit					1455:1459	The sensitivity and detection limit	1425:1459	limit	1455:1459	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	9	37	theme	detection	1445:1453	arg1	cm-2					1530:1533	466.7 μA mol-1 L cm-2	1513:1533	466.7 μA mol-1 L cm-2	1513:1533	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	6	38	theme	cellulose	924:932	arg1	film					934:937	the cellulose film	920:937	the cellulose film reacted with and without GOD or GOD-NL-CBM2	920:981	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2 confirmed the immobilization of GOD-NL-CBM2.					
34489028	3	39	theme	affinity	559:566	arg1	tag					579:581	an affinity adsorption tag	556:581	an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode	556:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	3	39	theme	affinity	559:566	arg1	CBM2					539:542	The CBM2	535:542	The CBM2	535:542	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	4	40	theme	type	728:731	arg1	GOD					733:735	the wild type GOD	719:735	the wild type GOD	719:735	The specific activity of GOD-NL-CBM2 was comparable to that of the wild type GOD.					
34489028	2	41	theme	GOD	414:416	arg1	terminal					402:409	the C terminal	396:409	the C terminal of GOD	396:416	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	8	42	theme	current	1303:1309	arg1	response					1318:1325	the current signal response	1299:1325	the current signal response of the biosensor to glucose	1299:1353	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	42	theme	current	1303:1309	arg1	linear					1359:1364	linear	1359:1364	linear	1359:1364	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	42	theme	current	1303:1309	arg1	r2					1413:1414	r2 ≥ 0.99	1413:1421	r2 ≥ 0.99	1413:1421	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	1	43	theme	biosensors	297:306	arg1	performance					274:284	performance	274:284	performance	274:284	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	1	43	theme	biosensors	297:306	arg1	fabrication					258:268	fabrication	258:268	fabrication	258:268	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	9	44	theme	GOD-NL-CBM2/cellulose	1468:1488	arg1	bioelectrode					1495:1506	the GOD-NL-CBM2/cellulose film bioelectrode	1464:1506	the GOD-NL-CBM2/cellulose film bioelectrode	1464:1506	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	7	45	theme	glucose	1171:1177	arg1	presence					1159:1166	the presence	1155:1166	the presence of glucose	1155:1177	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	10	46	theme	RSD	1698:1700	arg1	%					1705:1705	RSD < 5%	1698:1705	RSD < 5%	1698:1705	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	46	theme	RSD	1698:1700	arg1	deviation					1687:1695	Relative standard deviation	1669:1695	Relative standard deviation	1669:1695	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	9	47	theme	bioelectrode	1495:1506	arg1	limit					1455:1459	The sensitivity and detection limit	1425:1459	limit	1455:1459	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	9	47	theme	bioelectrode	1495:1506	arg1	cm-2					1530:1533	466.7 μA mol-1 L cm-2	1513:1533	466.7 μA mol-1 L cm-2	1513:1533	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	2	48	theme	module	347:352	arg1	family					354:359	a carbohydrate binding module family 2	324:361	a carbohydrate binding module family 2 (CBM2)	324:368	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	2	48	theme	module	347:352	arg1	CBM2					364:367	CBM2	364:367	CBM2	364:367	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	9	49	theme	466.7	1513:1517	arg1	μA					1519:1520	μA	1519:1520	μA	1519:1520	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	7	50	theme	characteristic	1114:1127	arg1	peak					1129:1132	a characteristic peak	1112:1132	a characteristic peak of H2O2 at +0.6 V in the presence of glucose	1112:1177	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	10	51	theme	substrate	1709:1717	arg1	selectivity					1719:1729	substrate selectivity	1709:1729	substrate selectivity	1709:1729	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	2	52	theme	carbohydrate	326:337	arg1	family					354:359	a carbohydrate binding module family 2	324:361	a carbohydrate binding module family 2 (CBM2)	324:368	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	2	52	theme	carbohydrate	326:337	arg1	CBM2					364:367	CBM2	364:367	CBM2	364:367	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	9	53	theme	mol-1	1522:1526	arg1	limit					1455:1459	The sensitivity and detection limit	1425:1459	limit	1455:1459	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	9	53	theme	mol-1	1522:1526	arg1	cm-2					1530:1533	466.7 μA mol-1 L cm-2	1513:1533	466.7 μA mol-1 L cm-2	1513:1533	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	10	54	dep	reproducibility	1652:1666	arg1	%					1705:1705	RSD < 5%	1698:1705	RSD < 5%	1698:1705	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	54	dep	reproducibility	1652:1666	arg1	deviation					1687:1695	Relative standard deviation	1669:1695	Relative standard deviation	1669:1695	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	7	55	from	V	1150:1150	arg1	peak					1129:1132	a characteristic peak	1112:1132	a characteristic peak of H2O2 at +0.6 V in the presence of glucose	1112:1177	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	5	56	theme	CBM2	755:758	arg1	tag					760:762	the CBM2 tag	751:762	the CBM2 tag of fusion GOD	751:776	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	11	57	theme	glucose	1941:1947	arg1	biosensor					1949:1957	a high performance glucose biosensor	1922:1957	a high performance glucose biosensor	1922:1957	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	3	58	theme	cellulose	626:634	arg1	electrode					645:653	a cellulose modified electrode	624:653	a cellulose modified electrode	624:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	10	59	theme	current	1623:1629	arg1	change					1631:1636	a rapid current change	1615:1636	a rapid current change (< 5 s)	1615:1644	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	59	theme	current	1623:1629	arg1	s					1643:1643	< 5 s	1639:1643	< 5 s	1639:1643	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	11	60	theme	high	1924:1927	arg1	biosensor					1949:1957	a high performance glucose biosensor	1922:1957	a high performance glucose biosensor	1922:1957	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	2	61	from	linker	433:438	arg1	endo-β-xylanase					448:462	endo-β-xylanase	448:462	endo-β-xylanase by genetic recombination	448:487	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	5	62	theme	fusion	767:772	arg1	GOD					774:776	fusion GOD	767:776	fusion GOD	767:776	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	1	63	theme	oxidase	198:204	arg1	immobilization					172:185	immobilization	172:185	immobilization of glucose oxidase (GOD) on electrodes	172:224	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	2	64	theme	genetic	467:473	arg1	recombination					475:487	genetic recombination	467:487	genetic recombination	467:487	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	0	65	theme	cellulose-modified	128:145	arg1	electrode					147:155	the cellulose-modified electrode	124:155	the cellulose-modified electrode	124:155	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	5	66	theme	highest	800:806	arg1	capacity					816:823	its highest binding capacity	796:823	its highest binding capacity	796:823	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	10	67	theme	high	1647:1650	arg1	reproducibility					1652:1666	high reproducibility	1647:1666	high reproducibility (Relative standard deviation, RSD < 5%)	1647:1706	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	8	68	theme	concentration	1373:1385	arg1	range					1387:1391	the concentration range	1369:1391	the concentration range from 1.25 to 40 mM	1369:1410	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	10	69	theme	response	1794:1801	arg1	response					1794:1801	the original current response	1773:1801	the original current response	1773:1801	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	69	theme	response	1794:1801	arg1	%					1768:1768	about 80 %	1759:1768	about 80 % of the original current response	1759:1801	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	1	70	from	immobilization	172:185	arg1	electrodes					215:224	electrodes	215:224	electrodes	215:224	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	5	71	theme	GOD	774:776	arg1	tag					760:762	the CBM2 tag	751:762	the CBM2 tag of fusion GOD	751:776	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	10	72	theme	original	1777:1784	arg1	response					1794:1801	the original current response	1773:1801	the original current response	1773:1801	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	0	73	theme	binding	69:75	arg1	family					84:89	a carbohydrate binding module family	54:89	a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode	54:155	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	1	74	from	method	162:167	arg1	electrodes					215:224	electrodes	215:224	electrodes	215:224	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	2	75	theme	natural	425:431	arg1	NL					441:442	NL	441:442	NL	441:442	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	2	75	theme	natural	425:431	arg1	linker					433:438	a natural linker	423:438	a natural linker (NL) in endo-β-xylanase by genetic recombination	423:487	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	7	76	theme	bioelectrode	1093:1104	arg1	properties					1048:1057	The electrochemical properties	1028:1057	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode	1028:1104	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	0	77	dep	family	84:89	arg1	tag					93:95	2 tag	91:95	a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode	54:155	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	7	78	theme	immobilized	1211:1221	arg1	GOD-NL-CBM2					1223:1233	the immobilized GOD-NL-CBM2	1207:1233	the immobilized GOD-NL-CBM2	1207:1233	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	0	79	theme	glucose	2:8	arg1	biosensor					10:18	A glucose biosensor	0:18	A glucose biosensor	0:18	A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode.					
34489028	5	80	theme	optimal	831:837	arg1	conditions					849:858	optimal catalytic conditions	831:858	optimal catalytic conditions (pH 5.0, 50 °C)	831:874	In addition, the CBM2 tag of fusion GOD almost maintained its highest binding capacity under optimal catalytic conditions (pH 5.0, 50 °C).					
34489028	2	81	theme	C	400:400	arg1	terminal					402:409	the C terminal	396:409	the C terminal of GOD	396:416	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	6	82	theme	film	934:937	arg1	analysis					908:915	The morphology and composition analysis	877:915	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2	877:981	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2 confirmed the immobilization of GOD-NL-CBM2.					
34489028	10	83	theme	standard	1678:1685	arg1	%					1705:1705	RSD < 5%	1698:1705	RSD < 5%	1698:1705	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	83	theme	standard	1678:1685	arg1	deviation					1687:1695	Relative standard deviation	1669:1695	Relative standard deviation	1669:1695	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	4	84	theme	specific	660:667	arg1	activity					669:676	The specific activity	656:676	The specific activity of GOD-NL-CBM2	656:691	The specific activity of GOD-NL-CBM2 was comparable to that of the wild type GOD.					
34489028	4	84	theme	specific	660:667	arg1	comparable					697:706	comparable	697:706	comparable	697:706	The specific activity of GOD-NL-CBM2 was comparable to that of the wild type GOD.					
34489028	7	85	theme	electrochemical	1032:1046	arg1	properties					1048:1057	The electrochemical properties	1028:1057	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode	1028:1104	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	8	86	dep	linear	1359:1364	arg1	response					1318:1325	the current signal response	1299:1325	the current signal response of the biosensor to glucose	1299:1353	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	86	dep	linear	1359:1364	arg1	linear					1359:1364	linear	1359:1364	linear	1359:1364	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	86	dep	linear	1359:1364	arg1	r2					1413:1414	r2 ≥ 0.99	1413:1421	r2 ≥ 0.99	1413:1421	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	4	87	theme	wild	723:726	arg1	GOD					733:735	the wild type GOD	719:735	the wild type GOD	719:735	The specific activity of GOD-NL-CBM2 was comparable to that of the wild type GOD.					
34489028	8	88	from	mM	1409:1410	arg1	range					1387:1391	the concentration range	1369:1391	the concentration range from 1.25 to 40 mM	1369:1410	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	1	89	theme	glucose	289:295	arg1	biosensors					297:306	glucose biosensors	289:306	glucose biosensors	289:306	The method of immobilization of glucose oxidase (GOD) on electrodes is especially important for the fabrication and performance of glucose biosensors.					
34489028	11	90	theme	promising	1892:1900	arg1	approach					1902:1909	a promising approach	1890:1909	a promising approach to develop a high performance glucose biosensor	1890:1957	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	6	91	theme	GOD-NL-CBM2	1015:1025	arg1	immobilization					997:1010	the immobilization	993:1010	the immobilization of GOD-NL-CBM2	993:1025	The morphology and composition analysis of the cellulose film reacted with and without GOD or GOD-NL-CBM2 confirmed the immobilization of GOD-NL-CBM2.					
34489028	11	92	theme	adsorption-based	1832:1847	arg1	strategy					1864:1871	The affinity adsorption-based immobilization strategy	1819:1871	The affinity adsorption-based immobilization strategy for GOD	1819:1879	The affinity adsorption-based immobilization strategy for GOD provides a promising approach to develop a high performance glucose biosensor.					
34489028	10	93	theme	<	1702:1702	arg1	%					1705:1705	RSD < 5%	1698:1705	RSD < 5%	1698:1705	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	10	93	theme	<	1702:1702	arg1	deviation					1687:1695	Relative standard deviation	1669:1695	Relative standard deviation	1669:1695	Moreover, the glucose biosensor exhibited a rapid current change (< 5 s), high reproducibility (Relative standard deviation, RSD < 5%), substrate selectivity and stability, and retained about 80 % of the original current response after 2 months.					
34489028	9	94	theme	film	1490:1493	arg1	bioelectrode					1495:1506	the GOD-NL-CBM2/cellulose film bioelectrode	1464:1506	the GOD-NL-CBM2/cellulose film bioelectrode	1464:1506	The sensitivity and detection limit of the GOD-NL-CBM2/cellulose film bioelectrode were 466.7 μA mol-1 L cm-2 and 0.475 mM (S/N = 3), respectively.					
34489028	3	95	theme	adsorption	568:577	arg1	tag					579:581	an affinity adsorption tag	556:581	an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode	556:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	3	95	theme	adsorption	568:577	arg1	CBM2					539:542	The CBM2	535:542	The CBM2	535:542	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	8	96	theme	signal	1311:1316	arg1	response					1318:1325	the current signal response	1299:1325	the current signal response of the biosensor to glucose	1299:1353	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	96	theme	signal	1311:1316	arg1	linear					1359:1364	linear	1359:1364	linear	1359:1364	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	8	96	theme	signal	1311:1316	arg1	r2					1413:1414	r2 ≥ 0.99	1413:1421	r2 ≥ 0.99	1413:1421	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34489028	2	97	theme	binding	339:345	arg1	family					354:359	a carbohydrate binding module family 2	324:361	a carbohydrate binding module family 2 (CBM2)	324:368	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	2	97	theme	binding	339:345	arg1	CBM2					364:367	CBM2	364:367	CBM2	364:367	In this study, a carbohydrate binding module family 2 (CBM2) was successfully fused to the C terminal of GOD with a natural linker (NL) in endo-β-xylanase by genetic recombination, and a fusion GOD (GOD-NL-CBM2) was obtained.					
34489028	3	98	theme	GOD-NL-CBM2	609:619	arg1	immobilization					587:600	immobilization	587:600	immobilization of the GOD-NL-CBM2 on a cellulose modified electrode	587:653	The CBM2 was used as an affinity adsorption tag for immobilization of the GOD-NL-CBM2 on a cellulose modified electrode.					
34489028	7	99	theme	GOD-NL-CBM2/cellulose	1066:1086	arg1	bioelectrode					1093:1104	the GOD-NL-CBM2/cellulose film bioelectrode	1062:1104	the GOD-NL-CBM2/cellulose film bioelectrode	1062:1104	The electrochemical properties of the GOD-NL-CBM2/cellulose film bioelectrode, with a characteristic peak of H2O2 at +0.6 V in the presence of glucose, revealed the capability of the immobilized GOD-NL-CBM2 to efficiently catalyze glucose and produce H2O2.					
34489028	8	100	dep	40	1406:1407	arg1	to					1403:1404	to	1403:1404	to	1403:1404	Additionally, the current signal response of the biosensor to glucose was linear in the concentration range from 1.25 to 40 mM (r2 ≥ 0.99).					
34704223	6	0	theme	NaCl	1053:1056	arg1	concentrations					1058:1071	NaCl concentrations	1053:1071	NaCl concentrations	1053:1071	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	3	1	theme	%	675:675	arg1	yield					663:667	a collagen yield	652:667	a collagen yield of 2.7%	652:675	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	3	2	theme	Cirrhinus	538:546	arg1	scales					556:561	Cirrhinus mrigala scales	538:561	Cirrhinus mrigala scales	538:561	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	5	3	theme	collagen	837:844	arg1	temperature					818:828	The denaturation temperature	801:828	The denaturation temperature of the collagen	801:844	The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC).					
34704223	5	4	theme	denaturation	805:816	arg1	temperature					818:828	The denaturation temperature	801:828	The denaturation temperature of the collagen	801:844	The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC).					
34704223	5	5	theme	differential	877:888	arg1	DSC					912:914	DSC	912:914	DSC	912:914	The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC).					
34704223	5	5	theme	differential	877:888	arg1	calorimetry					899:909	differential scanning calorimetry	877:909	differential scanning calorimetry (DSC)	877:915	The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC).					
34704223	8	6	dep	Pseudomonas	1558:1568	arg1	aeruginosa					1570:1579	aeruginosa	1570:1579	aeruginosa	1570:1579	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	1	7	theme	excellent	220:228	arg1	biocompatibility					230:245	its excellent biocompatibility	216:245	its excellent biocompatibility	216:245	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	8	8	theme	antimicrobial	1433:1445	arg1	activity					1447:1454	good antimicrobial activity	1428:1454	good antimicrobial activity	1428:1454	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	3	9	theme	acid-soluble	600:611	arg1	collagen					613:620	acid-soluble collagen	600:620	acid-soluble collagen (ASC) using acetic acid	600:644	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	3	9	theme	acid-soluble	600:611	arg1	ASC					623:625	ASC	623:625	ASC	623:625	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	3	10	dep	Cirrhinus	538:546	arg1	mrigala					548:554	mrigala	548:554	mrigala	548:554	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	4	11	theme	FTIR	704:707	arg1	analyses					709:716	FTIR analyses	704:716	FTIR analyses	704:716	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	4	11	theme	FTIR	704:707	arg1	spectra					685:691	UV-Vis spectra	678:691	UV-Vis spectra	678:691	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	8	12	theme	extract	1368:1374	arg1	films					1382:1386	collagen-chitosan-neem extract (CCN) films	1345:1386	collagen-chitosan-neem extract (CCN) films	1345:1386	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	8	13	from	potential	1607:1615	arg1	development					1636:1646	the development	1632:1646	the development of antimicrobial films	1632:1669	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	3	14	theme	acetic	634:639	arg1	acid					641:644	acetic acid	634:644	acetic acid	634:644	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	6	15	theme	NaCl	973:976	arg1	concentrations					978:991	lower NaCl concentrations	967:991	lower NaCl concentrations	967:991	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	1	16	theme	promising	148:156	arg1	Collagen					134:141	Collagen	134:141	Collagen	134:141	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	1	16	theme	promising	148:156	arg1	candidate					158:166	a promising candidate	146:166	a promising candidate for food and pharmaceutical applications	146:207	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	8	17	theme	physicochemical	1286:1300	arg1	characteristics					1302:1316	the good physicochemical characteristics	1277:1316	the good physicochemical characteristics of the extracted collagen	1277:1342	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	0	18	theme	antimicrobial	76:88	arg1	film					90:93	a biodegradable antimicrobial film	60:93	a biodegradable antimicrobial film	60:93	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	6	19	theme	acidic	953:958	arg1	pH					960:961	acidic pH	953:961	acidic pH	953:961	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	8	20	theme	Staphylococcus	1493:1506	arg1	NCIM					1515:1518	Staphylococcus aureus NCIM 2654	1493:1523	Staphylococcus aureus NCIM 2654	1493:1523	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	6	21	from	concentrations	978:991	arg1	collagen					922:929	The collagen	918:929	The collagen	918:929	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	6	21	from	concentrations	978:991	arg1	soluble					942:948	soluble	942:948	soluble	942:948	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	8	22	theme	extracted	1325:1333	arg1	collagen					1335:1342	the extracted collagen	1321:1342	the extracted collagen	1321:1342	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	0	23	theme	mrigala	110:116	arg1	collagen					124:131	Cirrhinus mrigala scale collagen	100:131	Cirrhinus mrigala scale collagen	100:131	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	6	24	theme	alkaline	1029:1036	arg1	conditions					1038:1047	alkaline conditions	1029:1047	alkaline conditions	1029:1047	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	6	25	from	pH	960:961	arg1	collagen					922:929	The collagen	918:929	The collagen	918:929	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	6	25	from	pH	960:961	arg1	soluble					942:948	soluble	942:948	soluble	942:948	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	0	26	theme	biodegradable	62:74	arg1	film					90:93	a biodegradable antimicrobial film	60:93	a biodegradable antimicrobial film	60:93	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	7	27	theme	activity	1153:1160	arg1	EAI					1169:1171	EAI	1169:1171	EAI	1169:1171	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	27	theme	activity	1153:1160	arg1	index					1162:1166	an emulsion activity index	1141:1166	an emulsion activity index (EAI)	1141:1172	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	4	28	theme	amino	723:727	arg1	composition					734:744	amino acid composition	723:744	amino acid composition	723:744	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	4	28	theme	amino	723:727	arg1	spectra					685:691	UV-Vis spectra	678:691	UV-Vis spectra	678:691	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	0	29	theme	biochemical	11:21	arg1	characterization					23:38	biochemical characterization	11:38	biochemical characterization	11:38	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	8	30	dep	Bacillus	1464:1471	arg1	subtilis					1473:1480	subtilis	1473:1480	subtilis	1473:1480	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	2	31	theme	promising	486:494	arg1	source					496:501	a promising source	484:501	a promising source of collagen	484:513	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	2	31	theme	promising	486:494	arg1	they					475:478	they	475:478	they	475:478	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	4	32	theme	collagen	781:788	arg1	nature					767:772	the type I nature	756:772	the type I nature of the collagen extracted	756:798	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	1	33	theme	heavy	312:316	arg1	price					318:322	its heavy price	308:322	its heavy price	308:322	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	7	34	theme	good	1109:1112	arg1	potential					1126:1134	good emulsifying potential	1109:1134	good emulsifying potential	1109:1134	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	35	theme	15.67 ± 0.13 min	1236:1251	arg1	EAI					1169:1171	EAI	1169:1171	EAI	1169:1171	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	35	theme	15.67 ± 0.13 min	1236:1251	arg1	index					1162:1166	an emulsion activity index	1141:1166	an emulsion activity index (EAI)	1141:1172	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	35	theme	15.67 ± 0.13 min	1236:1251	arg1	ESI					1204:1206	ESI	1204:1206	ESI	1204:1206	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	35	theme	15.67 ± 0.13 min	1236:1251	arg1	index					1197:1201	emulsion stability index	1178:1201	emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min	1178:1251	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	9	36	theme	biodegradable	1836:1848	arg1	films					1850:1854	novel biodegradable films	1830:1854	novel biodegradable films with antimicrobial efficacy	1830:1882	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	4	37	theme	I	765:765	arg1	nature					767:772	the type I nature	756:772	the type I nature of the collagen extracted	756:798	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	8	38	dep	Escherichia	1526:1536	arg1	coli					1538:1541	coli	1538:1541	coli	1538:1541	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	7	39	theme	m2 g-1	1225:1230	arg1	EAI					1169:1171	EAI	1169:1171	EAI	1169:1171	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	39	theme	m2 g-1	1225:1230	arg1	index					1162:1166	an emulsion activity index	1141:1166	an emulsion activity index (EAI)	1141:1172	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	39	theme	m2 g-1	1225:1230	arg1	ESI					1204:1206	ESI	1204:1206	ESI	1204:1206	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	39	theme	m2 g-1	1225:1230	arg1	index					1197:1201	emulsion stability index	1178:1201	emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min	1178:1251	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	3	40	theme	present	523:529	arg1	study					531:535	the present study	519:535	the present study	519:535	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	1	41	theme	low	248:250	arg1	antigenicity					252:263	low antigenicity	248:263	low antigenicity	248:263	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	0	42	from	collagen	124:131	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	0	42	from	collagen	124:131	arg1	characterization					23:38	biochemical characterization	11:38	biochemical characterization	11:38	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	0	42	from	collagen	124:131	arg1	development					45:55	development	45:55	development	45:55	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	8	43	theme	films	1665:1669	arg1	development					1636:1646	the development	1632:1646	the development of antimicrobial films	1632:1669	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	1	44	theme	controlled	270:279	arg1	biodegradability					281:296	controlled biodegradability	270:296	controlled biodegradability	270:296	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	8	45	theme	collagen	1620:1627	arg1	potential					1607:1615	the potential	1603:1615	the potential of collagen in the development of antimicrobial films	1603:1669	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	1	46	theme	food	172:175	arg1	applications					196:207	food and pharmaceutical applications	172:207	food and pharmaceutical applications	172:207	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	9	47	with	films	1850:1854	arg1	efficacy					1875:1882	antimicrobial efficacy	1861:1882	antimicrobial efficacy	1861:1882	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	7	48	theme	emulsion	1178:1185	arg1	ESI					1204:1206	ESI	1204:1206	ESI	1204:1206	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	48	theme	emulsion	1178:1185	arg1	index					1197:1201	emulsion stability index	1178:1201	emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min	1178:1251	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	1	49	theme	pharmaceutical	181:194	arg1	applications					196:207	food and pharmaceutical applications	172:207	food and pharmaceutical applications	172:207	Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization.					
34704223	8	50	theme	good	1428:1431	arg1	activity					1447:1454	good antimicrobial activity	1428:1454	good antimicrobial activity	1428:1454	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	8	51	theme	good	1281:1284	arg1	characteristics					1302:1316	the good physicochemical characteristics	1277:1316	the good physicochemical characteristics of the extracted collagen	1277:1342	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	9	52	theme	fish	1721:1724	arg1	waste					1726:1730	fish waste	1721:1730	fish waste	1721:1730	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	8	53	theme	Bacillus	1464:1471	arg1	2635					1487:1490	Bacillus subtilis NCIM 2635	1464:1490	Bacillus subtilis NCIM 2635	1464:1490	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	5	54	theme	scanning	890:897	arg1	DSC					912:914	DSC	912:914	DSC	912:914	The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC).					
34704223	5	54	theme	scanning	890:897	arg1	calorimetry					899:909	differential scanning calorimetry	877:909	differential scanning calorimetry (DSC)	877:915	The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC).					
34704223	9	55	from	waste	1726:1730	arg1	collagen					1707:1714	the collagen	1703:1714	the collagen from fish waste	1703:1730	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	8	56	theme	NCIM	1482:1485	arg1	2635					1487:1490	Bacillus subtilis NCIM 2635	1464:1490	Bacillus subtilis NCIM 2635	1464:1490	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	7	57	theme	stability	1187:1195	arg1	ESI					1204:1206	ESI	1204:1206	ESI	1204:1206	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	57	theme	stability	1187:1195	arg1	index					1197:1201	emulsion stability index	1178:1201	emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min	1178:1251	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	6	58	theme	lower	967:971	arg1	concentrations					978:991	lower NaCl concentrations	967:991	lower NaCl concentrations	967:991	The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M.					
34704223	2	59	theme	collagen	506:513	arg1	source					496:501	a promising source	484:501	a promising source of collagen	484:513	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	2	59	theme	collagen	506:513	arg1	they					475:478	they	475:478	they	475:478	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	0	60	theme	film	90:93	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	0	60	theme	film	90:93	arg1	characterization					23:38	biochemical characterization	11:38	biochemical characterization	11:38	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	0	60	theme	film	90:93	arg1	development					45:55	development	45:55	development	45:55	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	9	61	used	used	1755:1758	arg2	collagen					1707:1714	the collagen	1703:1714	the collagen from fish waste	1703:1730	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	8	62	theme	aureus	1508:1513	arg1	NCIM					1515:1518	Staphylococcus aureus NCIM 2654	1493:1523	Staphylococcus aureus NCIM 2654	1493:1523	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	4	63	theme	UV-Vis	678:683	arg1	SDS-PAGE					694:701	SDS-PAGE	694:701	SDS-PAGE	694:701	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	4	63	theme	UV-Vis	678:683	arg1	analyses					709:716	FTIR analyses	704:716	FTIR analyses	704:716	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	4	63	theme	UV-Vis	678:683	arg1	spectra					685:691	UV-Vis spectra	678:691	UV-Vis spectra	678:691	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	4	63	theme	UV-Vis	678:683	arg1	composition					734:744	amino acid composition	723:744	amino acid composition	723:744	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	0	64	theme	Cirrhinus	100:108	arg1	collagen					124:131	Cirrhinus mrigala scale collagen	100:131	Cirrhinus mrigala scale collagen	100:131	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	4	65	theme	acid	729:732	arg1	composition					734:744	amino acid composition	723:744	amino acid composition	723:744	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	4	65	theme	acid	729:732	arg1	spectra					685:691	UV-Vis spectra	678:691	UV-Vis spectra	678:691	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	0	66	theme	scale	118:122	arg1	collagen					124:131	Cirrhinus mrigala scale collagen	100:131	Cirrhinus mrigala scale collagen	100:131	Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.					
34704223	8	67	theme	Escherichia	1526:1536	arg1	2832					1548:1551	Escherichia coli NCIM 2832	1526:1551	Escherichia coli NCIM 2832	1526:1551	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	7	68	theme	emulsion	1144:1151	arg1	EAI					1169:1171	EAI	1169:1171	EAI	1169:1171	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	7	68	theme	emulsion	1144:1151	arg1	index					1162:1166	an emulsion activity index	1141:1166	an emulsion activity index (EAI)	1141:1172	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	9	69	theme	neem	1796:1799	arg1	extract					1801:1807	neem extract	1796:1807	neem extract	1796:1807	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	8	70	theme	NCIM	1543:1546	arg1	2832					1548:1551	Escherichia coli NCIM 2832	1526:1551	Escherichia coli NCIM 2832	1526:1551	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	8	71	theme	collagen	1335:1342	arg1	characteristics					1302:1316	the good physicochemical characteristics	1277:1316	the good physicochemical characteristics of the extracted collagen	1277:1342	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	2	72	theme	environmental	443:455	arg1	scales					356:361	Fish scales	351:361	Fish scales generated during the processing	351:393	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	2	72	theme	environmental	443:455	arg1	pollutant					457:465	an environmental pollutant	440:465	an environmental pollutant	440:465	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	8	73	theme	collagen-chitosan-neem	1345:1366	arg1	CCN					1377:1379	CCN	1377:1379	CCN	1377:1379	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	8	73	theme	collagen-chitosan-neem	1345:1366	arg1	extract					1368:1374	collagen-chitosan-neem extract	1345:1374	collagen-chitosan-neem extract (CCN) films	1345:1386	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	7	74	theme	emulsifying	1114:1124	arg1	potential					1126:1134	good emulsifying potential	1109:1134	good emulsifying potential	1109:1134	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	8	75	theme	Pseudomonas	1558:1568	arg1	5032					1586:1589	Pseudomonas aeruginosa NCIM 5032	1558:1589	Pseudomonas aeruginosa NCIM 5032	1558:1589	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	2	76	theme	waste	421:425	arg1	material					427:434	waste material	421:434	waste material	421:434	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	2	76	theme	waste	421:425	arg1	scales					356:361	Fish scales	351:361	Fish scales generated during the processing	351:393	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	9	77	theme	novel	1830:1834	arg1	films					1850:1854	novel biodegradable films	1830:1854	novel biodegradable films with antimicrobial efficacy	1830:1882	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	8	78	theme	NCIM	1581:1584	arg1	5032					1586:1589	Pseudomonas aeruginosa NCIM 5032	1558:1589	Pseudomonas aeruginosa NCIM 5032	1558:1589	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	2	79	theme	Fish	351:354	arg1	material					427:434	waste material	421:434	waste material	421:434	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	2	79	theme	Fish	351:354	arg1	scales					356:361	Fish scales	351:361	Fish scales generated during the processing	351:393	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	2	79	theme	Fish	351:354	arg1	pollutant					457:465	an environmental pollutant	440:465	an environmental pollutant	440:465	Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen.					
34704223	7	80	theme	21.49 ± 0.22	1212:1223	arg1	m2 g-1					1225:1230	21.49 ± 0.22 m2 g-1	1212:1230	21.49 ± 0.22 m2 g-1	1212:1230	The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively.					
34704223	9	81	theme	films	1850:1854	arg1	synthesis					1817:1825	the synthesis	1813:1825	the synthesis of novel biodegradable films with antimicrobial efficacy	1813:1882	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34704223	3	82	theme	collagen	654:661	arg1	yield					663:667	a collagen yield	652:667	a collagen yield of 2.7%	652:675	In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%.					
34704223	4	83	theme	type	760:763	arg1	nature					767:772	the type I nature	756:772	the type I nature of the collagen extracted	756:798	UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted.					
34704223	8	84	theme	antimicrobial	1651:1663	arg1	films					1665:1669	antimicrobial films	1651:1669	antimicrobial films	1651:1669	Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films.					
34704223	9	85	theme	antimicrobial	1861:1873	arg1	efficacy					1875:1882	antimicrobial efficacy	1861:1882	antimicrobial efficacy	1861:1882	These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.					
34116088	2	0	from	industry	399:406	arg1	use					331:333	the use	327:333	the use of starches, a recognized carbohydrate source, in the packaging industry	327:406	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	4	1	theme	starch	784:789	arg1	processing					791:800	starch processing	784:800	starch processing	784:800	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	3	2	theme	starchy	463:469	arg1	use					437:439	the use	433:439	the use of different residual starchy	433:469	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	1	3	theme	biodegradable	232:244	arg1	samples					261:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	8	4	theme	GS	1289:1290	arg1	addition					1292:1299	The GS addition	1285:1299	The GS addition	1285:1299	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	5	theme	percentage	1455:1464	arg1	values					1491:1496	the elongation percentage (160 ± 30% to 212 ± 14%) values	1440:1496	the elongation percentage (160 ± 30% to 212 ± 14%) values	1440:1496	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	6	theme	52 ± 13 MPa	1399:1409	arg1	10 ± 3 MPa					1414:1423	52 ± 13 MPa to 10 ± 3 MPa	1399:1423	52 ± 13 MPa to 10 ± 3 MPa	1399:1423	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	6	theme	52 ± 13 MPa	1399:1409	arg1	modulus					1390:1396	elastic modulus	1382:1396	elastic modulus (52 ± 13 MPa to 10 ± 3 MPa)	1382:1424	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	7	theme	tensile	1328:1334	arg1	1.2 ± 0.3 MPa					1363:1375	3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa	1346:1375	3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa	1346:1375	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	7	theme	tensile	1328:1334	arg1	strength					1336:1343	the tensile strength	1324:1343	the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa)	1324:1376	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	9	8	contain	containing	1605:1614	arg2	starch					1616:1621	starch	1616:1621	starch	1616:1621	It can be seen a high potential for the use of agro-industrial residues containing starch in bioplastic production.					
34116088	9	8	contain	containing	1605:1614	arg1	residues					1596:1603	agro-industrial residues	1580:1603	agro-industrial residues containing starch in bioplastic production	1580:1646	It can be seen a high potential for the use of agro-industrial residues containing starch in bioplastic production.					
34116088	8	9	theme	3.3 ± 0.1 MPa	1346:1358	arg1	1.2 ± 0.3 MPa					1363:1375	3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa	1346:1375	3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa	1346:1375	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	9	theme	3.3 ± 0.1 MPa	1346:1358	arg1	strength					1336:1343	the tensile strength	1324:1343	the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa)	1324:1376	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	2	10	from	use	331:333	arg1	industry					399:406	the packaging industry	385:406	the packaging industry	385:406	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	1	11	from	possible	161:168	arg1	general					146:152	general	146:152	general	146:152	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	3	12	theme	production	497:506	arg1	costs					488:492	the costs	484:492	the costs of production	484:506	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	5	13	theme	materials	877:885	arg1	characterization					887:902	The raw materials characterization	869:902	The raw materials characterization	869:902	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	4	14	theme	processing	791:800	arg1	step					776:779	the sieving step	764:779	the sieving step of starch processing	764:800	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	1	15	theme	starch-based	77:88	arg1	development					95:105	The starch-based film development	73:105	The starch-based film development	73:105	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	1	16	theme	tasteless	251:259	arg1	samples					261:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	7	17	theme	physicochemical	1242:1256	arg1	properties					1273:1282	the physicochemical and mechanical properties	1238:1282	the physicochemical and mechanical properties	1238:1282	The results were related to the physicochemical and mechanical properties.					
34116088	9	18	theme	residues	1596:1603	arg1	use					1573:1575	the use	1569:1575	the use of agro-industrial residues containing starch in bioplastic production	1569:1646	It can be seen a high potential for the use of agro-industrial residues containing starch in bioplastic production.					
34116088	1	19	theme	film	90:93	arg1	development					95:105	The starch-based film development	73:105	The starch-based film development	73:105	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	1	20	theme	transparent	180:190	arg1	samples					261:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	8	21	theme	elongation	1444:1453	arg1	%					1488:1488	160 ± 30% to 212 ± 14%	1467:1488	160 ± 30% to 212 ± 14%	1467:1488	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	21	theme	elongation	1444:1453	arg1	percentage					1455:1464	elongation percentage	1444:1464	the elongation percentage (160 ± 30% to 212 ± 14%) values	1440:1496	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	9	22	theme	high	1550:1553	arg1	potential					1555:1563	a high potential	1548:1563	a high potential for the use of agro-industrial residues containing starch in bioplastic production	1548:1646	It can be seen a high potential for the use of agro-industrial residues containing starch in bioplastic production.					
34116088	2	23	theme	carbohydrate	361:372	arg1	source					374:379	a recognized carbohydrate source	348:379	a recognized carbohydrate source	348:379	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	2	23	theme	carbohydrate	361:372	arg1	starches					338:345	starches	338:345	starches	338:345	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	7	24	theme	mechanical	1262:1271	arg1	properties					1273:1282	the physicochemical and mechanical properties	1238:1282	the physicochemical and mechanical properties	1238:1282	The results were related to the physicochemical and mechanical properties.					
34116088	1	25	from	general	146:152	arg1	possible					161:168	possible	161:168	possible	161:168	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	4	26	theme	starch	688:693	arg1	substitution					700:711	the cassava starch (CS) substitution	676:711	the cassava starch (CS) substitution	676:711	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	2	27	theme	recognized	350:359	arg1	source					374:379	a recognized carbohydrate source	348:379	a recognized carbohydrate source	348:379	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	2	27	theme	recognized	350:359	arg1	starches					338:345	starches	338:345	starches	338:345	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	4	28	theme	sieving	768:774	arg1	step					776:779	the sieving step	764:779	the sieving step of starch processing	764:800	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	4	29	theme	cassava	680:686	arg1	CS					696:697	CS	696:697	CS	696:697	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	4	29	theme	cassava	680:686	arg1	starch					688:693	cassava starch	680:693	the cassava starch (CS) substitution	676:711	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	8	30	dep	strength	1336:1343	arg1	values					1426:1431	values	1426:1431	values	1426:1431	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	4	31	theme	final	837:841	arg1	characteristics					852:866	the final material characteristics	833:866	the final material characteristics	833:866	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	2	32	from	starches	338:345	arg1	industry					399:406	the packaging industry	385:406	the packaging industry	385:406	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	2	33	theme	hunger-related	285:298	arg1	problems					300:307	world hunger-related problems	279:307	world hunger-related problems	279:307	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	2	33	theme	hunger-related	285:298	arg1	reality					315:321	a reality	313:321	a reality	313:321	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	8	34	theme	elastic	1382:1388	arg1	10 ± 3 MPa					1414:1423	52 ± 13 MPa to 10 ± 3 MPa	1399:1423	52 ± 13 MPa to 10 ± 3 MPa	1399:1423	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	34	theme	elastic	1382:1388	arg1	modulus					1390:1396	elastic modulus	1382:1396	elastic modulus (52 ± 13 MPa to 10 ± 3 MPa)	1382:1424	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	2	35	theme	packaging	389:397	arg1	industry					399:406	the packaging industry	385:406	the packaging industry	385:406	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	0	36	theme	residue	26:32	arg1	utilization					34:44	residue utilization	26:44	residue utilization	26:44	Cassava starch-processing residue utilization for packaging development.					
34116088	4	37	theme	gelatinized	716:726	arg1	GS					736:737	GS	736:737	GS	736:737	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	4	37	theme	gelatinized	716:726	arg1	starch					728:733	gelatinized starch	716:733	gelatinized starch (GS)	716:738	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	4	37	theme	gelatinized	716:726	arg1	residue					743:749	a residue	741:749	a residue generated in the sieving step of starch processing	741:800	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	2	38	theme	world	279:283	arg1	problems					300:307	world hunger-related problems	279:307	world hunger-related problems	279:307	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	2	38	theme	world	279:283	arg1	reality					315:321	a reality	313:321	a reality	313:321	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
34116088	0	39	theme	packaging	50:58	arg1	development					60:70	packaging development	50:70	packaging development	50:70	Cassava starch-processing residue utilization for packaging development.					
34116088	4	40	theme	present	633:639	arg1	work					641:644	the present work	629:644	the present work	629:644	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	6	41	theme	homogeneous	1187:1197	arg1	structure					1199:1207	continuous and homogeneous structure	1172:1207	continuous and homogeneous structure	1172:1207	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	5	42	theme	thermal	1017:1023	arg1	analysis					1025:1032	thermal analysis	1017:1032	thermal analysis	1017:1032	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	8	43	from	decrease	1312:1319	arg1	10 ± 3 MPa					1414:1423	52 ± 13 MPa to 10 ± 3 MPa	1399:1423	52 ± 13 MPa to 10 ± 3 MPa	1399:1423	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	43	from	decrease	1312:1319	arg1	1.2 ± 0.3 MPa					1363:1375	3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa	1346:1375	3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa	1346:1375	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	43	from	decrease	1312:1319	arg1	strength					1336:1343	the tensile strength	1324:1343	the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa)	1324:1376	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	43	from	decrease	1312:1319	arg1	modulus					1390:1396	elastic modulus	1382:1396	elastic modulus (52 ± 13 MPa to 10 ± 3 MPa)	1382:1424	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	1	44	theme	odorless	204:211	arg1	samples					261:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	4	45	theme	material	843:850	arg1	characteristics					852:866	the final material characteristics	833:866	the final material characteristics	833:866	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	3	46	theme	food	600:603	arg1	chain					605:609	the food chain	596:609	the food chain	596:609	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	5	47	theme	centesimal	961:970	arg1	composition					972:982	centesimal composition	961:982	centesimal composition	961:982	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	8	48	dep	10 ± 3 MPa	1414:1423	arg1	to					1411:1412	to	1411:1412	to	1411:1412	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	49	dep	1.2 ± 0.3 MPa	1363:1375	arg1	to					1360:1361	to	1360:1361	to	1360:1361	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	4	50	theme	possible	813:820	arg1	changes					822:828	possible changes	813:828	possible changes in the final material characteristics	813:866	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	5	51	theme	size	994:997	arg1	distribution					999:1010	particle size distribution	985:1010	particle size distribution	985:1010	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	3	52	theme	innovative	538:547	arg1	biomaterials					549:560	innovative biomaterials	538:560	innovative biomaterials	538:560	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	3	53	theme	different	444:452	arg1	starchy					463:469	different residual starchy	444:469	different residual starchy	444:469	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	8	54	theme	%	1475:1475	arg1	%					1488:1488	160 ± 30% to 212 ± 14%	1467:1488	160 ± 30% to 212 ± 14%	1467:1488	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	8	54	theme	%	1475:1475	arg1	percentage					1455:1464	elongation percentage	1444:1464	the elongation percentage (160 ± 30% to 212 ± 14%) values	1440:1496	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	6	55	theme	macroscopic	1128:1138	arg1	aspects					1156:1162	the visual macroscopic and microscopic aspects	1117:1162	the visual macroscopic and microscopic aspects showing continuous and homogeneous structure	1117:1207	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	9	56	theme	bioplastic	1626:1635	arg1	production					1637:1646	bioplastic production	1626:1646	bioplastic production	1626:1646	It can be seen a high potential for the use of agro-industrial residues containing starch in bioplastic production.					
34116088	5	57	theme	particle	985:992	arg1	distribution					999:1010	particle size distribution	985:1010	particle size distribution	985:1010	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	3	58	theme	biomaterials	549:560	arg1	development					523:533	the development	519:533	the development of innovative biomaterials	519:560	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	9	59	theme	agro-industrial	1580:1594	arg1	residues					1596:1603	agro-industrial residues	1580:1603	agro-industrial residues containing starch in bioplastic production	1580:1646	It can be seen a high potential for the use of agro-industrial residues containing starch in bioplastic production.					
34116088	3	60	theme	residual	454:461	arg1	starchy					463:469	different residual starchy	444:469	different residual starchy	444:469	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	6	61	theme	microscopic	1144:1154	arg1	aspects					1156:1162	the visual macroscopic and microscopic aspects	1117:1162	the visual macroscopic and microscopic aspects showing continuous and homogeneous structure	1117:1207	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	8	62	dep	%	1488:1488	arg1	to					1477:1478	to	1477:1478	to	1477:1478	The GS addition promoted a decrease in the tensile strength (3.3 ± 0.1 MPa to 1.2 ± 0.3 MPa) and elastic modulus (52 ± 13 MPa to 10 ± 3 MPa) values, while the elongation percentage (160 ± 30% to 212 ± 14%) values seem to have not been so affected.					
34116088	4	63	from	changes	822:828	arg1	characteristics					852:866	the final material characteristics	833:866	the final material characteristics	833:866	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	5	64	theme	amylose	930:936	arg1	distribution					999:1010	particle size distribution	985:1010	particle size distribution	985:1010	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	64	theme	amylose	930:936	arg1	composition					972:982	centesimal composition	961:982	centesimal composition	961:982	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	64	theme	amylose	930:936	arg1	determination					913:925	determination	913:925	determination of amylose and moisture contents	913:958	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	64	theme	amylose	930:936	arg1	analysis					1025:1032	thermal analysis	1017:1032	thermal analysis	1017:1032	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	65	theme	raw	873:875	arg1	materials					877:885	raw materials	873:885	The raw materials characterization	869:902	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	1	66	theme	good	214:217	arg1	barrier					223:229	good gas barrier	214:229	good gas barrier	214:229	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	6	67	theme	drying	1062:1067	arg1	processes					1069:1077	solution and drying processes	1049:1077	solution and drying processes	1049:1077	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	6	68	theme	continuous	1172:1181	arg1	structure					1199:1207	continuous and homogeneous structure	1172:1207	continuous and homogeneous structure	1172:1207	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	4	69	theme	substitution	700:711	arg1	impact					666:671	the impact	662:671	the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing	662:800	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	4	70	theme	work	641:644	arg1	objective					616:624	The objective	612:624	The objective of the present work	612:644	The objective of the present work was to evaluate the impact of the cassava starch (CS) substitution by gelatinized starch (GS), a residue generated in the sieving step of starch processing, verifying possible changes in the final material characteristics.					
34116088	1	71	theme	gas	219:221	arg1	barrier					223:229	good gas barrier	214:229	good gas barrier	214:229	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	6	72	theme	solution	1049:1056	arg1	processes					1069:1077	solution and drying processes	1049:1077	solution and drying processes	1049:1077	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	5	73	theme	moisture	942:949	arg1	contents					951:958	moisture contents	942:958	moisture contents	942:958	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	1	74	theme	non-toxic	193:201	arg1	samples					261:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	6	75	theme	visual	1121:1126	arg1	aspects					1156:1162	the visual macroscopic and microscopic aspects	1117:1162	the visual macroscopic and microscopic aspects showing continuous and homogeneous structure	1117:1207	After casting solution and drying processes, the samples were evaluated regarding the visual macroscopic and microscopic aspects showing continuous and homogeneous structure.					
34116088	1	76	theme	barrier	223:229	arg1	samples					261:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples	180:267	The starch-based film development has been extensively studied since, in general, it is possible to obtain transparent, non-toxic, odorless, good gas barrier, biodegradable, and tasteless samples.					
34116088	3	77	with	competition	579:589	arg1	chain					605:609	the food chain	596:609	the food chain	596:609	Thus, the use of different residual starchy can minimize the costs of production, promoting the development of innovative biomaterials, eliminating the competition with the food chain.					
34116088	5	78	theme	contents	951:958	arg1	distribution					999:1010	particle size distribution	985:1010	particle size distribution	985:1010	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	78	theme	contents	951:958	arg1	composition					972:982	centesimal composition	961:982	centesimal composition	961:982	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	78	theme	contents	951:958	arg1	determination					913:925	determination	913:925	determination of amylose and moisture contents	913:958	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	5	78	theme	contents	951:958	arg1	analysis					1025:1032	thermal analysis	1017:1032	thermal analysis	1017:1032	The raw materials characterization includes determination of amylose and moisture contents, centesimal composition, particle size distribution, and thermal analysis.					
34116088	2	79	theme	starches	338:345	arg1	use					331:333	the use	327:333	the use of starches, a recognized carbohydrate source, in the packaging industry	327:406	However, world hunger-related problems are a reality and the use of starches, a recognized carbohydrate source, in the packaging industry should be avoided.					
33822378	8	0	theme	starch	949:954	arg1	film					956:959	the starch film	945:959	the starch film	945:959	Contrastingly, the thermal stability of the starch film was slightly decreased.					
33822378	14	1	theme	food	2260:2263	arg1	packaging					2265:2273	food packaging	2260:2273	food packaging	2260:2273	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	12	2	theme	pilot-scale	1852:1862	arg1	unit					1875:1878	a pilot-scale lamination unit	1850:1878	a pilot-scale lamination unit	1850:1878	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.					
33822378	11	3	theme	quaternary	1469:1478	arg1	films					1502:1506	quaternary active, biodegradable films	1469:1506	quaternary active, biodegradable films produced in a pilot-scale lamination unit	1469:1548	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	5	4	theme	LEO	709:711	arg1	addition					693:700	The addition	689:700	The addition of CB, LEO, and BSG	689:720	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	4	5	theme	mechanical	536:545	arg1	properties					547:556	the morphological, thermal, mechanical properties	508:556	properties	547:556	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	4	5	theme	mechanical	536:545	arg1	composition					495:505	The chemical composition	482:505	The chemical composition	482:505	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	9	6	theme	developed	1020:1028	arg1	films					1055:1059	the herein developed quaternary nanocomposite films	1009:1059	the herein developed quaternary nanocomposite films	1009:1059	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	1	7	attach	derived	261:267	arg2	CB					298:299	CB	298:299	CB	298:299	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	7	attach	derived	261:267	arg2	w/v					256:258	TPS, 8% w/v	248:258	w/v	256:258	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	7	attach	derived	261:267	arg1	cassava					274:280	cassava	274:280	cassava	274:280	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	7	attach	derived	261:267	arg2	starch					240:245	thermoplastic starch	226:245	thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	226:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	7	attach	derived	261:267	arg1	butter					289:294	cocoa butter	283:294	cocoa butter	283:294	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	5	8	dep	%	826:826	arg1	2.4-BSG5					811:818	2.4-BSG5	811:818	2.4-BSG5	811:818	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	9	9	theme	nanocomposite	1041:1053	arg1	films					1055:1059	the herein developed quaternary nanocomposite films	1009:1059	the herein developed quaternary nanocomposite films	1009:1059	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	15	10	theme	circular	2360:2367	arg1	framework					2380:2388	the circular bioeconomy framework	2356:2388	the circular bioeconomy framework	2356:2388	The valorization of a BSG also adds to the relevance of our contribution within the circular bioeconomy framework.					
33822378	1	11	theme	novel	180:184	arg1	films					211:215	novel quaternary nanocomposite films	180:215	novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	180:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	4	12	theme	morphological	512:524	arg1	properties					547:556	the morphological, thermal, mechanical properties	508:556	properties	547:556	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	4	12	theme	morphological	512:524	arg1	composition					495:505	The chemical composition	482:505	The chemical composition	482:505	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	3	13	theme	continuous	462:471	arg1	casting					473:479	continuous casting	462:479	continuous casting	462:479	%) fibers, by continuous casting.					
33822378	1	14	theme	nanocomposite	197:209	arg1	films					211:215	novel quaternary nanocomposite films	180:215	novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	180:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	12	15	theme	new	1801:1803	arg1	approach					1805:1812	new approach	1801:1812	new approach toward quaternary films produced in a pilot-scale lamination unit	1801:1878	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.					
33822378	11	16	from	successful	1554:1563	arg1	either					1568:1573	either	1568:1573	either	1568:1573	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	11	16	from	successful	1554:1563	arg1	at					1588:1589	at	1588:1589	at	1588:1589	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	11	16	from	successful	1554:1563	arg1	maintaining					1597:1607	maintaining	1597:1607	maintaining the essential properties of TPS-based films for food packaging applications	1597:1683	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	9	17	theme	active	1145:1150	arg1	ingredients					1152:1162	active ingredients	1145:1162	active ingredients that are expected to have some biocidal effect	1145:1209	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	11	18	theme	TPS-based	1637:1645	arg1	films					1647:1651	TPS-based films	1637:1651	TPS-based films for food packaging applications	1637:1683	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	11	19	from	either	1568:1573	arg1	successful					1554:1563	successful	1554:1563	successful	1554:1563	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	9	20	with	supplementation	1124:1138	arg1	ingredients					1152:1162	active ingredients	1145:1162	active ingredients that are expected to have some biocidal effect	1145:1209	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	14	21	theme	passive	2138:2144	arg1	barrier					2146:2152	both a passive barrier	2131:2152	barrier	2146:2152	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	9	22	theme	TPS	1085:1087	arg1	films					1089:1093	TPS films	1085:1093	TPS films	1085:1093	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	2	23	theme	different	383:391	arg1	concentrations					393:406	different concentrations	383:406	different concentrations of brewery spent grain (BSG, 5 or 10 wt	383:446	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	0	24	theme	cocoa	68:72	arg1	butter					74:79	cocoa butter	68:79	cocoa butter	68:79	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	12	25	theme	PRACTICAL	1749:1757	arg1	APPLICATION					1759:1769	PRACTICAL APPLICATION	1749:1769	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.	1749:1879	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.					
33822378	4	26	from	application	636:646	arg1	chocolates					651:660	chocolates	651:660	chocolates	651:660	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	14	27	theme	properties	2104:2113	arg1	performance					2115:2125	properties performance	2104:2125	properties performance	2104:2125	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	2	28	dep	nanoemulsions	353:365	arg1	reinforced					367:376	reinforced	367:376	nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt	353:446	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	29	theme	spent	419:423	arg1	5					437:437	5	437:437	5	437:437	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	29	theme	spent	419:423	arg1	BSG					432:434	brewery spent grain (BSG	411:434	brewery spent grain (BSG	411:434	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	29	theme	spent	419:423	arg1	10 wt					442:446	10 wt	442:446	10 wt	442:446	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	0	30	theme	essential	93:101	arg1	nanoemulsion					107:118	lemongrass essential oil nanoemulsion	82:118	lemongrass essential oil nanoemulsion	82:118	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	2	31	dep	LEO	343:345	arg1	1:1					348:350	1:1	348:350	1:1	348:350	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	14	32	from	good	2096:2099	arg1	performance					2115:2125	properties performance	2104:2125	properties performance	2104:2125	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	2	33	theme	essential	328:336	arg1	LEO					343:345	LEO	343:345	LEO	343:345	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	33	theme	essential	328:336	arg1	oil					338:340	lemongrass essential oil	317:340	lemongrass essential oil (LEO, 1:1)	317:351	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	0	34	theme	quaternary	15:24	arg1	nanocomposites					26:39	quaternary nanocomposites	15:39	quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery	15:131	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	5	35	theme	increased	781:789	arg1	extensibility					791:803	increased extensibility	781:803	increased extensibility (from 2.4-BSG5 to 9.4%-BSG10)	781:833	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	14	36	theme	films	2061:2065	arg1	components					2036:2045	The four components	2027:2045	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.	2027:2274	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	1	37	theme	cocoa	283:287	arg1	cassava					274:280	cassava	274:280	cassava	274:280	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	37	theme	cocoa	283:287	arg1	butter					289:294	cocoa butter	283:294	cocoa butter	283:294	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	14	38	theme	sensory	2205:2211	arg1	attributes					2213:2222	the sensory attributes	2201:2222	the sensory attributes of food	2201:2230	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	11	39	theme	packaging	1662:1670	arg1	applications					1672:1683	food packaging applications	1657:1683	food packaging applications	1657:1683	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	1	40	theme	films	211:215	arg1	production					166:175	production	166:175	production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	166:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	14	41	theme	edible	2054:2059	arg1	films					2061:2065	the edible films	2050:2065	the edible films	2050:2065	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	11	42	theme	lamination	1534:1543	arg1	unit					1545:1548	a pilot-scale lamination unit	1520:1548	a pilot-scale lamination unit	1520:1548	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	15	43	theme	contribution	2336:2347	arg1	relevance					2319:2327	the relevance	2315:2327	the relevance of our contribution within the circular bioeconomy framework	2315:2388	The valorization of a BSG also adds to the relevance of our contribution within the circular bioeconomy framework.					
33822378	12	44	theme	lamination	1864:1873	arg1	unit					1875:1878	a pilot-scale lamination unit	1850:1878	a pilot-scale lamination unit	1850:1878	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.					
33822378	14	45	dep	barrier	2146:2152	arg1	both					2131:2134	both	2131:2134	both	2131:2134	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	10	46	theme	chocolate	1391:1399	arg1	consumers					1348:1356	consumers	1348:1356	consumers	1348:1356	Despite checking antimicrobial activity only by contact under the biocomposites, chocolates packed with the films were well accepted by consumers, especially the samples of white chocolate stored in the BSG5 biocomposite.					
33822378	10	46	theme	chocolate	1391:1399	arg1	samples					1374:1380	the samples	1370:1380	the samples of white chocolate stored in the BSG5 biocomposite	1370:1431	Despite checking antimicrobial activity only by contact under the biocomposites, chocolates packed with the films were well accepted by consumers, especially the samples of white chocolate stored in the BSG5 biocomposite.					
33822378	11	47	theme	new	1448:1450	arg1	approach					1452:1459	this new approach	1443:1459	this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit	1443:1548	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	1	48	theme	TPS	248:250	arg1	w/v					256:258	TPS, 8% w/v	248:258	w/v	256:258	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	48	theme	TPS	248:250	arg1	starch					240:245	thermoplastic starch	226:245	thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	226:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	8	49	theme	film	956:959	arg1	stability					932:940	the thermal stability	920:940	the thermal stability of the starch film	920:959	Contrastingly, the thermal stability of the starch film was slightly decreased.					
33822378	10	50	theme	BSG5	1415:1418	arg1	biocomposite					1420:1431	the BSG5 biocomposite	1411:1431	the BSG5 biocomposite	1411:1431	Despite checking antimicrobial activity only by contact under the biocomposites, chocolates packed with the films were well accepted by consumers, especially the samples of white chocolate stored in the BSG5 biocomposite.					
33822378	11	51	theme	active	1480:1485	arg1	films					1502:1506	quaternary active, biodegradable films	1469:1506	quaternary active, biodegradable films produced in a pilot-scale lamination unit	1469:1548	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	10	52	theme	antimicrobial	1229:1241	arg1	activity					1243:1250	checking antimicrobial activity	1220:1250	checking antimicrobial activity only by contact under the biocomposites	1220:1290	Despite checking antimicrobial activity only by contact under the biocomposites, chocolates packed with the films were well accepted by consumers, especially the samples of white chocolate stored in the BSG5 biocomposite.					
33822378	10	53	theme	checking	1220:1227	arg1	activity					1243:1250	checking antimicrobial activity	1220:1250	checking antimicrobial activity only by contact under the biocomposites	1220:1290	Despite checking antimicrobial activity only by contact under the biocomposites, chocolates packed with the films were well accepted by consumers, especially the samples of white chocolate stored in the BSG5 biocomposite.					
33822378	9	54	theme	quaternary	1030:1039	arg1	films					1055:1059	the herein developed quaternary nanocomposite films	1009:1059	the herein developed quaternary nanocomposite films	1009:1059	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	12	55	theme	quaternary	1821:1830	arg1	films					1832:1836	quaternary films	1821:1836	quaternary films produced in a pilot-scale lamination unit	1821:1878	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.					
33822378	11	56	from	at	1588:1589	arg1	successful					1554:1563	successful	1554:1563	successful	1554:1563	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	11	57	theme	essential	1613:1621	arg1	properties					1623:1632	the essential properties	1609:1632	the essential properties of TPS-based films for food packaging applications	1609:1683	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	9	58	theme	films	1055:1059	arg1	biodegradability					989:1004	The biodegradability	985:1004	The biodegradability of the herein developed quaternary nanocomposite films	985:1059	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	9	58	theme	films	1055:1059	arg1	same					1069:1072	same	1069:1072	same	1069:1072	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	4	59	from	composition	495:505	arg1	compound					607:614	the vegetable compound	593:614	the vegetable compound	593:614	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	1	60	theme	quaternary	186:195	arg1	films					211:215	novel quaternary nanocomposite films	180:215	novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	180:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	2	61	theme	BSG	432:434	arg1	concentrations					393:406	different concentrations	383:406	different concentrations of brewery spent grain (BSG, 5 or 10 wt	383:446	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	12	62	dep	APPLICATION	1759:1769	arg1	relates					1790:1796	relates	1790:1796	relates to new approach toward quaternary films produced in a pilot-scale lamination unit	1790:1878	PRACTICAL APPLICATION: This contribution relates to new approach toward quaternary films produced in a pilot-scale lamination unit.					
33822378	11	63	theme	food	1657:1660	arg1	applications					1672:1683	food packaging applications	1657:1683	food packaging applications	1657:1683	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	0	64	theme	grain	139:143	arg1	fibers					145:150	grain fibers	139:150	grain fibers	139:150	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	11	65	theme	films	1647:1651	arg1	properties					1623:1632	the essential properties	1609:1632	the essential properties of TPS-based films for food packaging applications	1609:1683	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	5	66	theme	starch-based	753:764	arg1	films					766:770	the starch-based films	749:770	the starch-based films	749:770	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	13	67	theme	plant	1951:1955	arg1	biomass					1957:1963	plant biomass	1951:1963	plant biomass	1951:1963	It relates to sustainability as it is both biodegradable and based on plant biomass, as well as produced via a clean, through high-yield process.					
33822378	5	68	theme	BSG	718:720	arg1	addition					693:700	The addition	689:700	The addition of CB, LEO, and BSG	689:720	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	14	69	dep	components	2036:2045	arg1	developed					2070:2078	developed	2070:2078	developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging	2070:2273	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	5	70	theme	relevant	729:736	arg1	changes					738:744	relevant changes	729:744	relevant changes	729:744	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	5	70	theme	relevant	729:736	arg1	extensibility					791:803	increased extensibility	781:803	increased extensibility (from 2.4-BSG5 to 9.4%-BSG10)	781:833	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	5	70	theme	relevant	729:736	arg1	barrier					848:854	improved barrier	839:854	improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1	839:890	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	4	71	theme	film	559:562	arg1	composition					495:505	The chemical composition	482:505	The chemical composition	482:505	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	4	71	theme	film	559:562	arg1	barrier					564:570	film barrier	559:570	film barrier	559:570	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	0	72	theme	lemongrass	82:91	arg1	nanoemulsion					107:118	lemongrass essential oil nanoemulsion	82:118	lemongrass essential oil nanoemulsion	82:118	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	5	73	from	changes	738:744	arg1	films					766:770	the starch-based films	749:770	the starch-based films	749:770	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	2	74	theme	grain	425:429	arg1	5					437:437	5	437:437	5	437:437	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	74	theme	grain	425:429	arg1	BSG					432:434	brewery spent grain (BSG	411:434	brewery spent grain (BSG	411:434	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	74	theme	grain	425:429	arg1	10 wt					442:446	10 wt	442:446	10 wt	442:446	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	14	75	dep	physical	2167:2174	arg1	i.e.					2155:2158	i.e.	2155:2158	i.e.	2155:2158	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	0	76	theme	oil	103:105	arg1	nanoemulsion					107:118	lemongrass essential oil nanoemulsion	82:118	lemongrass essential oil nanoemulsion	82:118	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	15	77	theme	BSG	2298:2300	arg1	valorization					2280:2291	The valorization	2276:2291	The valorization of a BSG	2276:2300	The valorization of a BSG also adds to the relevance of our contribution within the circular bioeconomy framework.					
33822378	2	78	theme	brewery	411:417	arg1	5					437:437	5	437:437	5	437:437	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	78	theme	brewery	411:417	arg1	BSG					432:434	brewery spent grain (BSG	411:434	brewery spent grain (BSG	411:434	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	78	theme	brewery	411:417	arg1	10 wt					442:446	10 wt	442:446	10 wt	442:446	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	1	79	dep	derived	261:267	arg1	starch					240:245	thermoplastic starch	226:245	thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	226:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	79	dep	derived	261:267	arg1	CB					298:299	CB	298:299	CB	298:299	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	79	dep	derived	261:267	arg1	%					304:304	30% wt	302:307	30% wt	302:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	4	80	theme	vegetable	597:605	arg1	compound					607:614	the vegetable compound	593:614	the vegetable compound	593:614	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	0	81	theme	nanocomposites	26:39	arg1	Development					0:10	Development	0:10	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery	0:131	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	1	82	theme	%	254:254	arg1	w/v					256:258	TPS, 8% w/v	248:258	w/v	256:258	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	82	theme	%	254:254	arg1	starch					240:245	thermoplastic starch	226:245	thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	226:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	15	83	theme	bioeconomy	2369:2378	arg1	framework					2380:2388	the circular bioeconomy framework	2356:2388	the circular bioeconomy framework	2356:2388	The valorization of a BSG also adds to the relevance of our contribution within the circular bioeconomy framework.					
33822378	2	84	theme	lemongrass	317:326	arg1	LEO					343:345	LEO	343:345	LEO	343:345	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	2	84	theme	lemongrass	317:326	arg1	oil					338:340	lemongrass essential oil	317:340	lemongrass essential oil (LEO, 1:1)	317:351	%), and lemongrass essential oil (LEO, 1:1) nanoemulsions reinforced with different concentrations of brewery spent grain (BSG, 5 or 10 wt.					
33822378	4	85	dep	morphological	512:524	arg1	thermal					527:533	thermal	527:533	thermal	527:533	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	14	86	theme	food	2227:2230	arg1	attributes					2213:2222	the sensory attributes	2201:2222	the sensory attributes of food	2201:2230	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	0	87	theme	cassava	52:58	arg1	starch					60:65	cassava starch	52:65	cassava starch	52:65	Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers.					
33822378	11	88	dep	active	1480:1485	arg1	biodegradable					1488:1500	biodegradable	1488:1500	biodegradable	1488:1500	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	5	89	theme	improved	839:846	arg1	barrier					848:854	improved barrier	839:854	improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1	839:890	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	11	90	from	maintaining	1597:1607	arg1	successful					1554:1563	successful	1554:1563	successful	1554:1563	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	8	91	theme	thermal	924:930	arg1	stability					932:940	the thermal stability	920:940	the thermal stability of the starch film	920:959	Contrastingly, the thermal stability of the starch film was slightly decreased.					
33822378	11	92	theme	pilot-scale	1522:1532	arg1	unit					1545:1548	a pilot-scale lamination unit	1520:1548	a pilot-scale lamination unit	1520:1548	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	9	93	contain	have	1185:1188	arg1	ingredients					1152:1162	active ingredients	1145:1162	active ingredients that are expected to have some biocidal effect	1145:1209	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	9	93	contain	have	1185:1188	arg2	effect					1204:1209	some biocidal effect	1190:1209	some biocidal effect	1190:1209	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	9	94	theme	biocidal	1195:1202	arg1	effect					1204:1209	some biocidal effect	1190:1209	some biocidal effect	1190:1209	The biodegradability of the herein developed quaternary nanocomposite films was the same as that of TPS films, eliminating concerns on the supplementation with active ingredients that are expected to have some biocidal effect.					
33822378	5	95	theme	CB	705:706	arg1	addition					693:700	The addition	689:700	The addition of CB, LEO, and BSG	689:720	The addition of CB, LEO, and BSG caused relevant changes in the starch-based films, such as increased extensibility (from 2.4-BSG5 to 9.4%-BSG10) and improved barrier to moisture (2.9 and 2.4 g.mm.kPa-1 .					
33822378	14	96	from	performance	2115:2125	arg1	good					2096:2099	good	2096:2099	good	2096:2099	The four components of the edible films we developed provide it with good in properties performance, as both a passive barrier (i.e. purely physical), and active, related to the sensory attributes of food, essential to be applied in food packaging.					
33822378	4	97	theme	chemical	486:493	arg1	biodegradability					573:588	biodegradability	573:588	biodegradability	573:588	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	4	97	theme	chemical	486:493	arg1	composition					495:505	The chemical composition	482:505	The chemical composition	482:505	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	4	97	theme	chemical	486:493	arg1	barrier					564:570	film barrier	559:570	film barrier	559:570	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	4	97	theme	chemical	486:493	arg1	properties					547:556	the morphological, thermal, mechanical properties	508:556	properties	547:556	The chemical composition, the morphological, thermal, mechanical properties, film barrier, biodegradability in the vegetable compound, in addition to the application in chocolates, have been widely studied.					
33822378	1	98	theme	thermoplastic	226:238	arg1	w/v					256:258	TPS, 8% w/v	248:258	w/v	256:258	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	98	theme	thermoplastic	226:238	arg1	CB					298:299	CB	298:299	CB	298:299	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	1	98	theme	thermoplastic	226:238	arg1	starch					240:245	thermoplastic starch	226:245	thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt	226:307	We report on production of novel quaternary nanocomposite films based on thermoplastic starch (TPS, 8% w/v) derived from cassava, cocoa butter, (CB, 30% wt.					
33822378	11	99	theme	unique	1712:1717	arg1	features					1719:1726	unique features	1712:1726	unique features	1712:1726	Overall, this new approach towards quaternary active, biodegradable films produced in a pilot-scale lamination unit was successful in either improving or at least maintaining the essential properties of TPS-based films for food packaging applications, while providing them with unique features and functionalities.					
33822378	13	100	theme	high-yield	2007:2016	arg1	process					2018:2024	high-yield process	2007:2024	high-yield process	2007:2024	It relates to sustainability as it is both biodegradable and based on plant biomass, as well as produced via a clean, through high-yield process.					
32799163	5	0	theme	functional	809:818	arg1	properties					833:842	functional and physical properties	809:842	functional and physical properties	809:842	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	4	1	theme	increasing	551:560	arg1	content					566:572	the content	562:572	increasing the content of defatted algal biomass	551:598	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	0	2	theme	Functional	70:79	arg1	characterization					94:109	Functional and physical characterization	70:109	Functional and physical characterization	70:109	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	4	3	theme	films	679:683	arg1	permeability					653:664	water vapor permeability	641:664	water vapor permeability of composite films	641:683	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	1	4	theme	thermal	242:248	arg1	stability					250:258	thermal stability	242:258	thermal stability	242:258	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	5	theme	homogeneous	865:875	arg1	distribution					889:900	the homogeneous and uniform distribution	861:900	the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents	861:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	3	6	theme	films	531:535	arg1	content					455:461	moisture content	446:461	moisture content	446:461	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	3	6	theme	films	531:535	arg1	swelling					467:474	swelling	467:474	swelling	467:474	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	1	7	theme	stability	250:258	arg1	terms					233:237	terms	233:237	terms of thermal stability, mechanical, water barrier, and optical properties	233:309	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	0	8	theme	physical	85:92	arg1	characterization					94:109	Functional and physical characterization	70:109	Functional and physical characterization	70:109	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	5	9	from	improvement	794:804	arg1	properties					833:842	functional and physical properties	809:842	functional and physical properties	809:842	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	6	10	from	%	1102:1102	arg1	test					1142:1145	60 days soil burial test	1122:1145	60 days soil burial test	1122:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	3	11	theme	Cs/DCB	523:528	arg1	films					531:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	0	12	dep	characterization	94:109	arg1	films					14:18	Biodegradable films	0:18	Biodegradable films based on chitosan and defatted Chlorella biomass	0:67	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	1	13	theme	defatted	165:172	arg1	biomass					184:190	defatted Chlorella biomass	165:190	defatted Chlorella biomass (DCB)	165:196	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	1	13	theme	defatted	165:172	arg1	DCB					193:195	DCB	193:195	DCB	193:195	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	14	theme	uniform	881:887	arg1	distribution					889:900	the homogeneous and uniform distribution	861:900	the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents	861:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	3	15	theme	biomass	514:520	arg1	films					531:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	1	16	theme	Chlorella	174:182	arg1	biomass					184:190	defatted Chlorella biomass	165:190	defatted Chlorella biomass (DCB)	165:196	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	1	16	theme	Chlorella	174:182	arg1	DCB					193:195	DCB	193:195	DCB	193:195	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	17	dep	Fourier	752:758	arg1	transform					760:768	transform	760:768	transform infrared analysis	760:786	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	3	18	dep	content	455:461	arg1	degree					476:481	degree	476:481	degree	476:481	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	3	19	theme	Chlorella	504:512	arg1	films					531:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	6	20	theme	Cs/DCB	1060:1065	arg1	films					1077:1081	Cs/DCB composite films	1060:1081	Cs/DCB composite films	1060:1081	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	0	21	theme	Biodegradable	0:12	arg1	films					14:18	Biodegradable films	0:18	Biodegradable films based on chitosan and defatted Chlorella biomass	0:67	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	1	22	theme	mechanical	261:270	arg1	terms					233:237	terms	233:237	terms of thermal stability, mechanical, water barrier, and optical properties	233:309	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	23	dep	transform	760:768	arg1	infrared					770:777	infrared	770:777	transform infrared analysis	760:786	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	4	24	theme	water	641:645	arg1	permeability					653:664	water vapor permeability	641:664	water vapor permeability of composite films	641:683	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	5	25	theme	polymeric	918:926	arg1	matrix					928:933	the polymeric matrix	914:933	the polymeric matrix	914:933	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	4	26	theme	vapor	647:651	arg1	permeability					653:664	water vapor permeability	641:664	water vapor permeability of composite films	641:683	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	5	27	theme	such	789:792	arg1	improvement					794:804	such improvement	789:804	such improvement in functional and physical properties	789:842	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	6	28	theme	soil	1130:1133	arg1	test					1142:1145	60 days soil burial test	1122:1145	60 days soil burial test	1122:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	6	29	theme	composite	1067:1075	arg1	films					1077:1081	Cs/DCB composite films	1060:1081	Cs/DCB composite films	1060:1081	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	3	30	theme	DCB	427:429	arg1	incorporation					410:422	The incorporation	406:422	The incorporation of DCB	406:429	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	6	31	theme	burial	1135:1140	arg1	test					1142:1145	60 days soil burial test	1122:1145	60 days soil burial test	1122:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	1	32	theme	water	273:277	arg1	barrier					279:285	water barrier	273:285	water barrier	273:285	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	2	33	dep	25 wt	345:349	arg1	to					342:343	to	342:343	to	342:343	Increasing DCB content from 5 to 25 wt% increased tensile strength of chitosan films by 235%.					
32799163	1	34	theme	Biodegradable	112:124	arg1	films					126:130	Biodegradable films	112:130	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB)	112:196	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	6	35	from	test	1142:1145	arg1	degradation					1107:1117	degradation	1107:1117	degradation in 60 days soil burial test	1107:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	6	35	from	test	1142:1145	arg1	%					1102:1102	more than 50%	1090:1102	more than 50% of degradation in 60 days soil burial test	1090:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	2	36	theme	films	391:395	arg1	strength					370:377	tensile strength	362:377	tensile strength of chitosan films	362:395	Increasing DCB content from 5 to 25 wt% increased tensile strength of chitosan films by 235%.					
32799163	1	37	theme	barrier	279:285	arg1	terms					233:237	terms	233:237	terms of thermal stability, mechanical, water barrier, and optical properties	233:309	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	38	theme	physical	824:831	arg1	properties					833:842	functional and physical properties	809:842	functional and physical properties	809:842	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	2	39	theme	chitosan	382:389	arg1	films					391:395	chitosan films	382:395	chitosan films	382:395	Increasing DCB content from 5 to 25 wt% increased tensile strength of chitosan films by 235%.					
32799163	0	40	theme	chitosan	29:36	arg1	biomass					61:67	chitosan and defatted Chlorella biomass	29:67	chitosan and defatted Chlorella biomass	29:67	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	5	41	theme	chitosan	1009:1016	arg1	constituents					1036:1047	chitosan and algal biomass constituents	1009:1047	chitosan and algal biomass constituents	1009:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	0	42	theme	Chlorella	51:59	arg1	biomass					61:67	chitosan and defatted Chlorella biomass	29:67	chitosan and defatted Chlorella biomass	29:67	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	6	43	theme	degradation	1107:1117	arg1	degradation					1107:1117	degradation	1107:1117	degradation in 60 days soil burial test	1107:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	6	43	theme	degradation	1107:1117	arg1	%					1102:1102	more than 50%	1090:1102	more than 50% of degradation in 60 days soil burial test	1090:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	0	44	theme	defatted	42:49	arg1	biomass					61:67	chitosan and defatted Chlorella biomass	29:67	chitosan and defatted Chlorella biomass	29:67	Biodegradable films based on chitosan and defatted Chlorella biomass: Functional and physical characterization.					
32799163	1	45	theme	optical	292:298	arg1	properties					300:309	optical properties	292:309	optical properties	292:309	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	46	theme	biomass	1028:1034	arg1	constituents					1036:1047	chitosan and algal biomass constituents	1009:1047	chitosan and algal biomass constituents	1009:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	5	47	theme	algal	1022:1026	arg1	constituents					1036:1047	chitosan and algal biomass constituents	1009:1047	chitosan and algal biomass constituents	1009:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	2	48	theme	DCB	323:325	arg1	content					327:333	DCB content	323:333	DCB content	323:333	Increasing DCB content from 5 to 25 wt% increased tensile strength of chitosan films by 235%.					
32799163	1	49	theme	properties	300:309	arg1	terms					233:237	terms	233:237	terms of thermal stability, mechanical, water barrier, and optical properties	233:309	Biodegradable films based on chitosan, glycerol, and defatted Chlorella biomass (DCB) were prepared and characterized in terms of thermal stability, mechanical, water barrier, and optical properties.					
32799163	5	50	theme	strong	967:972	arg1	interactions					988:999	strong hydrogen bond interactions	967:999	strong hydrogen bond interactions between chitosan and algal biomass constituents	967:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	6	51	from	degradation	1107:1117	arg1	test					1142:1145	60 days soil burial test	1122:1145	60 days soil burial test	1122:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	5	52	theme	DCB	905:907	arg1	distribution					889:900	the homogeneous and uniform distribution	861:900	the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents	861:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	4	53	theme	defatted	577:584	arg1	biomass					592:598	defatted algal biomass	577:598	defatted algal biomass	577:598	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	5	54	theme	hydrogen	974:981	arg1	interactions					988:999	strong hydrogen bond interactions	967:999	strong hydrogen bond interactions between chitosan and algal biomass constituents	967:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	3	55	theme	moisture	446:453	arg1	content					455:461	moisture content	446:461	moisture content	446:461	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	5	56	theme	scanning	719:726	arg1	microscopy					737:746	scanning electron microscopy	719:746	scanning electron microscopy	719:746	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	5	57	theme	bond	983:986	arg1	interactions					988:999	strong hydrogen bond interactions	967:999	strong hydrogen bond interactions between chitosan and algal biomass constituents	967:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	4	58	theme	light	610:614	arg1	transmission					616:627	light transmission	610:627	light transmission	610:627	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	4	59	theme	composite	669:677	arg1	films					679:683	composite films	669:683	composite films	669:683	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	2	60	theme	tensile	362:368	arg1	strength					370:377	tensile strength	362:377	tensile strength of chitosan films	362:395	Increasing DCB content from 5 to 25 wt% increased tensile strength of chitosan films by 235%.					
32799163	4	61	theme	biomass	592:598	arg1	content					566:572	the content	562:572	increasing the content of defatted algal biomass	551:598	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32799163	5	62	theme	electron	728:735	arg1	microscopy					737:746	scanning electron microscopy	719:746	scanning electron microscopy	719:746	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	6	63	theme	60 days	1122:1128	arg1	test					1142:1145	60 days soil burial test	1122:1145	60 days soil burial test	1122:1145	Moreover, Cs/DCB composite films showed more than 50% of degradation in 60 days soil burial test.					
32799163	5	64	theme	interactions	988:999	arg1	establishment					950:962	the establishment	946:962	the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents	946:1047	As confirmed by scanning electron microscopy and Fourier transform infrared analysis, such improvement in functional and physical properties is mainly due to the homogeneous and uniform distribution of DCB into the polymeric matrix along with the establishment of strong hydrogen bond interactions between chitosan and algal biomass constituents.					
32799163	3	65	theme	chitosan/defatted	486:502	arg1	films					531:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	chitosan/defatted Chlorella biomass (Cs/DCB) films	486:535	The incorporation of DCB decreased both moisture content and swelling degree of chitosan/defatted Chlorella biomass (Cs/DCB) films.					
32799163	4	66	theme	algal	586:590	arg1	biomass					592:598	defatted algal biomass	577:598	defatted algal biomass	577:598	Furthermore, increasing the content of defatted algal biomass decreased light transmission and reduced water vapor permeability of composite films by more than 60%.					
32311538	7	0	theme	hydrogel	1251:1258	arg1	microspheres					1260:1271	hydrogel microspheres	1251:1271	hydrogel microspheres	1251:1271	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	3	1	theme	nerve	633:637	arg1	tissue					639:644	native nerve tissue	626:644	native nerve tissue	626:644	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	3	2	theme	bioprinted	515:524	arg1	scaffold					537:544	a 3D bioprinted multiscale scaffold	510:544	a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue	510:644	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	7	3	theme	composite	1227:1235	arg1	scaffold					1237:1244	such multiscale composite scaffold	1211:1244	such multiscale composite scaffold with hydrogel microspheres	1211:1271	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	6	4	theme	Schwann	1015:1021	arg1	cells					1023:1027	RSC96 Schwann cells	1009:1027	RSC96 Schwann cells	1009:1027	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	0	5	theme	GelMA	76:80	arg1	microspheres					92:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	7	6	theme	great	1529:1533	arg1	applications					1545:1556	the great potential applications	1525:1556	the great potential applications in nerve tissue engineering	1525:1584	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	7	theme	cell	1483:1486	arg1	organization					1488:1499	nerve cell organization	1477:1499	nerve cell organization	1477:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	7	theme	cell	1483:1486	arg1	cells					1453:1457	Schwann cells	1445:1457	Schwann cells proliferation and nerve cell organization	1445:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	3	8	theme	multiscale	526:535	arg1	scaffold					537:544	a 3D bioprinted multiscale scaffold	510:544	a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue	510:644	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	7	9	theme	potential	1535:1543	arg1	applications					1545:1556	the great potential applications	1525:1556	the great potential applications in nerve tissue engineering	1525:1584	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	10	theme	epineurium	1424:1433	arg1	layer					1435:1439	the epineurium layer	1420:1439	the epineurium layer for Schwann cells proliferation and nerve cell organization	1420:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	3	11	theme	modular	557:563	arg1	bioink					565:570	a modular bioink	555:570	a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue	555:644	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	4	12	theme	/Chitosan	675:683	arg1	GC-MSs					699:704	GC-MSs	699:704	GC-MSs	699:704	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	12	theme	/Chitosan	675:683	arg1	Microspheres					685:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres	647:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs)	647:705	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	1	13	theme	critical	261:268	arg1	integration					180:190	The integration	176:190	The integration of multiscale micro- and macroenvironment	176:232	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	1	13	theme	critical	261:268	arg1	role					270:273	a critical role	259:273	a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration	259:348	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	0	14	theme	/chitosan	82:90	arg1	microspheres					92:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	7	15	theme	3D	1293:1294	arg1	microenvironment					1296:1311	a suitable 3D microenvironment	1282:1311	a suitable 3D microenvironment for enhancing neurite growth	1282:1340	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	6	16	theme	multiscale	1106:1115	arg1	environment					1117:1127	such a 3D multiscale environment	1096:1127	such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation	1096:1180	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	5	17	theme	3D	868:869	arg1	bioink					968:973	the modular bioink	956:973	the modular bioink	956:973	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	5	17	theme	3D	868:869	arg1	scaffolds					892:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	7	18	theme	multiscale	1216:1225	arg1	scaffold					1237:1244	such multiscale composite scaffold	1211:1244	such multiscale composite scaffold with hydrogel microspheres	1211:1271	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	5	19	theme	modular	960:966	arg1	bioink					968:973	the modular bioink	956:973	the modular bioink	956:973	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	5	19	theme	modular	960:966	arg1	scaffolds					892:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	4	20	theme	Gelatin	647:653	arg1	GC-MSs					699:704	GC-MSs	699:704	GC-MSs	699:704	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	20	theme	Gelatin	647:653	arg1	Microspheres					685:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres	647:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs)	647:705	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	6	21	theme	3D	1042:1043	arg1	scaffolds					1056:1064	these 3D biomimetic scaffolds	1036:1064	these 3D biomimetic scaffolds	1036:1064	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	7	22	theme	hydrogel	1362:1369	arg1	network					1371:1377	the 3D printed hydrogel network	1347:1377	the 3D printed hydrogel network	1347:1377	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	2	23	theme	biomimetic	408:417	arg1	scaffold					430:437	a biomimetic multiscale scaffold	406:437	a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth	406:488	While it remains a remarkable challenge for developing a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth.					
32311538	6	24	theme	cells	999:1003	arg1	co-culture					980:989	The co-culture	976:989	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds	976:1064	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	7	25	dep	cells	1453:1457	arg1	organization					1488:1499	nerve cell organization	1477:1499	nerve cell organization	1477:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	25	dep	cells	1453:1457	arg1	cells					1453:1457	Schwann cells	1445:1457	Schwann cells proliferation and nerve cell organization	1445:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	25	dep	cells	1453:1457	arg1	proliferation					1459:1471	proliferation	1459:1471	proliferation	1459:1471	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	4	26	from	effect	757:762	arg1	outgrowth					807:815	enhancing neurite outgrowth	789:815	enhancing neurite outgrowth	789:815	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	26	from	effect	757:762	arg1	elongation					821:830	elongation	821:830	elongation	821:830	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	27	theme	microspheres	773:784	arg1	effect					757:762	the effect	753:762	the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells	753:844	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	6	28	theme	3D	1103:1104	arg1	environment					1117:1127	such a 3D multiscale environment	1096:1127	such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation	1096:1180	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	5	29	theme	multiscale	871:880	arg1	bioink					968:973	the modular bioink	956:973	the modular bioink	956:973	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	5	29	theme	multiscale	871:880	arg1	scaffolds					892:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	7	30	theme	3D	1390:1391	arg1	macroenvironment					1393:1408	a 3D macroenvironment	1388:1408	a 3D macroenvironment	1388:1408	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	30	theme	3D	1390:1391	arg1	promising					1511:1519	promising	1511:1519	promising	1511:1519	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	31	theme	printed	1354:1360	arg1	network					1371:1377	the 3D printed hydrogel network	1347:1377	the 3D printed hydrogel network	1347:1377	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	32	theme	neurite	1327:1333	arg1	growth					1335:1340	neurite growth	1327:1340	neurite growth	1327:1340	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	5	33	theme	composite	882:890	arg1	bioink					968:973	the modular bioink	956:973	the modular bioink	956:973	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	5	33	theme	composite	882:890	arg1	scaffolds					892:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds	864:900	The 3D multiscale composite scaffolds were bioprinted based on microspheres and hydrogel as the modular bioink.					
32311538	7	34	theme	such	1211:1214	arg1	scaffold					1237:1244	such multiscale composite scaffold	1211:1244	such multiscale composite scaffold with hydrogel microspheres	1211:1271	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	6	35	theme	PC12	994:997	arg1	cells					999:1003	PC12 cells	994:1003	PC12 cells	994:1003	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	7	36	theme	tissue	1567:1572	arg1	engineering					1574:1584	nerve tissue engineering	1561:1584	nerve tissue engineering	1561:1584	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	3	37	theme	3D	592:593	arg1	micro-					595:600	3D micro-	592:600	3D micro-	592:600	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	0	38	theme	composite	25:33	arg1	bioink					118:123	a modular bioink	108:123	a modular bioink for enhancing 3D neurite outgrowth and elongation	108:173	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	0	38	theme	composite	25:33	arg1	scaffolds					35:43	multiscale composite scaffolds	14:43	multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres	14:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	1	39	theme	designing	278:286	arg1	scaffolds					299:307	designing biomimetic scaffolds	278:307	designing biomimetic scaffolds	278:307	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	4	40	theme	methacryloyl	655:666	arg1	GC-MSs					699:704	GC-MSs	699:704	GC-MSs	699:704	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	40	theme	methacryloyl	655:666	arg1	Microspheres					685:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres	647:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs)	647:705	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	0	41	theme	multiscale	14:23	arg1	bioink					118:123	a modular bioink	108:123	a modular bioink for enhancing 3D neurite outgrowth and elongation	108:173	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	0	41	theme	multiscale	14:23	arg1	scaffolds					35:43	multiscale composite scaffolds	14:43	multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres	14:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	1	42	theme	biomimetic	288:297	arg1	scaffolds					299:307	designing biomimetic scaffolds	278:307	designing biomimetic scaffolds	278:307	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	0	43	theme	modular	110:116	arg1	bioink					118:123	a modular bioink	108:123	a modular bioink for enhancing 3D neurite outgrowth and elongation	108:173	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	0	43	theme	modular	110:116	arg1	scaffolds					35:43	multiscale composite scaffolds	14:43	multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres	14:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	6	44	theme	cell	1163:1166	arg1	proliferation					1168:1180	Schwann cell proliferation	1155:1180	Schwann cell proliferation	1155:1180	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	1	45	theme	multiscale	195:204	arg1	micro-					206:211	multiscale micro-	195:211	multiscale micro-	195:211	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	7	46	with	scaffold	1237:1244	arg1	microspheres					1260:1271	hydrogel microspheres	1251:1271	hydrogel microspheres	1251:1271	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	4	47	theme	GelMA	669:673	arg1	GC-MSs					699:704	GC-MSs	699:704	GC-MSs	699:704	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	47	theme	GelMA	669:673	arg1	Microspheres					685:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres	647:696	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs)	647:705	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	1	48	theme	micro-	206:211	arg1	integration					180:190	The integration	176:190	The integration of multiscale micro- and macroenvironment	176:232	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	1	48	theme	micro-	206:211	arg1	role					270:273	a critical role	259:273	a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration	259:348	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	3	49	dep	micro-	595:600	arg1	the					588:590	the	588:590	the	588:590	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	2	50	theme	neuronal	456:463	arg1	maturation					465:474	maturation	465:474	maturation	465:474	While it remains a remarkable challenge for developing a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth.					
32311538	2	51	theme	multiscale	419:428	arg1	scaffold					430:437	a biomimetic multiscale scaffold	406:437	a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth	406:488	While it remains a remarkable challenge for developing a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth.					
32311538	0	52	theme	gelatin	54:60	arg1	microspheres					92:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	1	53	theme	peripheral	313:322	arg1	regeneration					337:348	peripheral nerve tissue regeneration	313:348	peripheral nerve tissue regeneration	313:348	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	0	54	theme	neurite	142:148	arg1	outgrowth					150:158	3D neurite outgrowth	139:158	3D neurite outgrowth	139:158	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	4	55	theme	cells	840:844	arg1	outgrowth					807:815	enhancing neurite outgrowth	789:815	enhancing neurite outgrowth	789:815	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	55	theme	cells	840:844	arg1	elongation					821:830	elongation	821:830	elongation	821:830	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	6	56	theme	Schwann	1155:1161	arg1	cell					1163:1166	Schwann cell	1155:1166	Schwann cell proliferation	1155:1180	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	1	57	theme	nerve	324:328	arg1	regeneration					337:348	peripheral nerve tissue regeneration	313:348	peripheral nerve tissue regeneration	313:348	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	0	58	theme	3D	139:140	arg1	outgrowth					150:158	3D neurite outgrowth	139:158	3D neurite outgrowth	139:158	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	4	59	theme	PC12	835:838	arg1	cells					840:844	PC12 cells	835:844	PC12 cells	835:844	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	3	60	theme	3D	512:513	arg1	scaffold					537:544	a 3D bioprinted multiscale scaffold	510:544	a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue	510:644	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	1	61	theme	tissue	330:335	arg1	regeneration					337:348	peripheral nerve tissue regeneration	313:348	peripheral nerve tissue regeneration	313:348	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	4	62	theme	neurite	799:805	arg1	outgrowth					807:815	enhancing neurite outgrowth	789:815	enhancing neurite outgrowth	789:815	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	3	63	theme	tissue	639:644	arg1	macroenvironment					606:621	macroenvironment	606:621	macroenvironment	606:621	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	3	63	theme	tissue	639:644	arg1	micro-					595:600	3D micro-	592:600	3D micro-	592:600	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	7	64	theme	Schwann	1445:1451	arg1	organization					1488:1499	nerve cell organization	1477:1499	nerve cell organization	1477:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	64	theme	Schwann	1445:1451	arg1	cells					1453:1457	Schwann cells	1445:1457	Schwann cells proliferation and nerve cell organization	1445:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	64	theme	Schwann	1445:1451	arg1	proliferation					1459:1471	proliferation	1459:1471	proliferation	1459:1471	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	6	65	theme	neurite	1133:1139	arg1	outgrowth					1141:1149	neurite outgrowth	1133:1149	neurite outgrowth	1133:1149	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	1	66	theme	macroenvironment	217:232	arg1	integration					180:190	The integration	176:190	The integration of multiscale micro- and macroenvironment	176:232	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	1	66	theme	macroenvironment	217:232	arg1	role					270:273	a critical role	259:273	a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration	259:348	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	7	67	theme	nerve	1477:1481	arg1	organization					1488:1499	nerve cell organization	1477:1499	nerve cell organization	1477:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	67	theme	nerve	1477:1481	arg1	cells					1453:1457	Schwann cells	1445:1457	Schwann cells proliferation and nerve cell organization	1445:1499	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	7	68	theme	nerve	1561:1565	arg1	engineering					1574:1584	nerve tissue engineering	1561:1584	nerve tissue engineering	1561:1584	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	4	69	theme	enhancing	789:797	arg1	outgrowth					807:815	enhancing neurite outgrowth	789:815	enhancing neurite outgrowth	789:815	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	4	70	theme	microfluidic	726:737	arg1	approach					739:746	a microfluidic approach	724:746	a microfluidic approach	724:746	Gelatin methacryloyl (GelMA)/Chitosan Microspheres (GC-MSs) were prepared by a microfluidic approach, and the effect of these microspheres on enhancing neurite outgrowth and elongation of PC12 cells was demonstrated.					
32311538	6	71	theme	biomimetic	1045:1054	arg1	scaffolds					1056:1064	these 3D biomimetic scaffolds	1036:1064	these 3D biomimetic scaffolds	1036:1064	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32311538	3	72	theme	native	626:631	arg1	tissue					639:644	native nerve tissue	626:644	native nerve tissue	626:644	Herein, we present a 3D bioprinted multiscale scaffold based on a modular bioink for integrating the 3D micro- and macroenvironment of native nerve tissue.					
32311538	7	73	theme	3D	1351:1352	arg1	network					1371:1377	the 3D printed hydrogel network	1347:1377	the 3D printed hydrogel network	1347:1377	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	1	74	from	role	270:273	arg1	scaffolds					299:307	designing biomimetic scaffolds	278:307	designing biomimetic scaffolds	278:307	The integration of multiscale micro- and macroenvironment has been demonstrated as a critical role in designing biomimetic scaffolds for peripheral nerve tissue regeneration.					
32311538	0	75	theme	methacryloyl	62:73	arg1	microspheres					92:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	gelatin methacryloyl (GelMA)/chitosan microspheres	54:103	3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation.					
32311538	7	76	from	applications	1545:1556	arg1	engineering					1574:1584	nerve tissue engineering	1561:1584	nerve tissue engineering	1561:1584	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	2	77	theme	3D	453:454	arg1	maturation					465:474	maturation	465:474	maturation	465:474	While it remains a remarkable challenge for developing a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth.					
32311538	2	78	theme	remarkable	370:379	arg1	challenge					381:389	a remarkable challenge	368:389	a remarkable challenge for developing a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth	368:488	While it remains a remarkable challenge for developing a biomimetic multiscale scaffold for enhancing 3D neuronal maturation and outgrowth.					
32311538	7	79	theme	suitable	1284:1291	arg1	microenvironment					1296:1311	a suitable 3D microenvironment	1282:1311	a suitable 3D microenvironment for enhancing neurite growth	1282:1340	These results indicate that such multiscale composite scaffold with hydrogel microspheres provided a suitable 3D microenvironment for enhancing neurite growth, and the 3D printed hydrogel network provided a 3D macroenvironment mimicking the epineurium layer for Schwann cells proliferation and nerve cell organization, which is promising for the great potential applications in nerve tissue engineering.					
32311538	6	80	theme	cells	1023:1027	arg1	co-culture					980:989	The co-culture	976:989	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds	976:1064	The co-culture of PC12 cells and RSC96 Schwann cells within these 3D biomimetic scaffolds were investigated to evaluate such a 3D multiscale environment for neurite outgrowth and Schwann cell proliferation.					
32092235	5	0	theme	cytokines	861:869	arg1	down-regulation					825:839	down-regulation	825:839	down-regulation of pro-inflammatory cytokines induced by lipopolysaccharides	825:900	Furthermore, YGPs/MTX can suppress the proliferation of macrophage cells efficiently, leading to down-regulation of pro-inflammatory cytokines induced by lipopolysaccharides.					
32092235	7	1	theme	MTX	1180:1182	arg1	application					1184:1194	MTX application	1180:1194	MTX application	1180:1194	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	3	2	theme	IBD	450:452	arg1	mice					454:457	IBD mice	450:457	IBD mice	450:457	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	5	3	theme	pro-inflammatory	844:859	arg1	cytokines					861:869	pro-inflammatory cytokines	844:869	pro-inflammatory cytokines induced by lipopolysaccharides	844:900	Furthermore, YGPs/MTX can suppress the proliferation of macrophage cells efficiently, leading to down-regulation of pro-inflammatory cytokines induced by lipopolysaccharides.					
32092235	7	4	theme	new	1163:1165	arg1	strategy					1167:1174	a new strategy	1161:1174	a new strategy for MTX application	1161:1194	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	2	5	theme	effective	253:261	arg1	treatments					263:272	more effective treatments	248:272	more effective treatments	248:272	There is a great clinical need for more effective treatments for IBD.					
32092235	6	6	theme	mice	969:972	arg1	site					953:956	the inflammation site	936:956	the inflammation site of colitis mice	936:972	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	6	6	theme	mice	969:972	arg1	mice					969:972	colitis mice	961:972	colitis mice	961:972	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	0	7	theme	Methotrexate	82:93	arg1	Delivery					70:77	Targeting Delivery	60:77	Targeting Delivery of Methotrexate through Glucan Particles	60:118	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.					
32092235	7	8	theme	clinical	1203:1210	arg1	treatment					1212:1220	the clinical treatment	1199:1220	the clinical treatment of IBD	1199:1227	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	4	9	theme	dectin-1	700:707	arg1	receptors					717:725	dectin-1 and CR3 receptors	700:725	dectin-1 and CR3 receptors	700:725	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	6	10	theme	colitis	961:967	arg1	mice					969:972	colitis mice	961:972	colitis mice	961:972	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	3	11	theme	oral	302:305	arg1	system					316:321	a targeted oral delivery system	291:321	a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	291:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	6	12	theme	MTX	1100:1102	arg1	cytotoxicity					1084:1095	the cytotoxicity	1080:1095	the cytotoxicity of MTX	1080:1102	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	5	13	theme	macrophage	784:793	arg1	cells					795:799	macrophage cells	784:799	macrophage cells	784:799	Furthermore, YGPs/MTX can suppress the proliferation of macrophage cells efficiently, leading to down-regulation of pro-inflammatory cytokines induced by lipopolysaccharides.					
32092235	3	14	contain	carrying	356:363	arg1	particles					339:347	yeast glucan particles	326:347	yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	326:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	14	contain	carrying	356:363	arg2	MTX					420:422	MTX	420:422	MTX	420:422	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	14	contain	carrying	356:363	arg2	methotrexate					406:417	a clinically used anti-inflammatory drug methotrexate	365:417	a clinically used anti-inflammatory drug methotrexate (MTX)	365:423	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	14	contain	carrying	356:363	arg1	YGPs					350:353	YGPs	350:353	YGPs	350:353	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	15	theme	anti-inflammatory	383:399	arg1	MTX					420:422	MTX	420:422	MTX	420:422	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	15	theme	anti-inflammatory	383:399	arg1	methotrexate					406:417	a clinically used anti-inflammatory drug methotrexate	365:417	a clinically used anti-inflammatory drug methotrexate (MTX)	365:423	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	0	16	theme	Glucan	103:108	arg1	Particles					110:118	Glucan Particles	103:118	Glucan Particles	103:118	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.					
32092235	4	17	theme	CR3	713:715	arg1	receptors					717:725	dectin-1 and CR3 receptors	700:725	dectin-1 and CR3 receptors	700:725	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	3	18	theme	drug	401:404	arg1	MTX					420:422	MTX	420:422	MTX	420:422	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	18	theme	drug	401:404	arg1	methotrexate					406:417	a clinically used anti-inflammatory drug methotrexate	365:417	a clinically used anti-inflammatory drug methotrexate (MTX)	365:423	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	0	19	theme	Novel	2:6	arg1	Strategy					8:15	A Novel Strategy	0:15	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.	0:119	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.					
32092235	6	20	dep	target	996:1001	arg1	reduce					1073:1078	reduce	1073:1078	reduce the cytotoxicity of MTX	1073:1102	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	6	20	dep	target	996:1001	arg1	improve					1040:1046	improve	1040:1046	improve the efficacy of MTX	1040:1066	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	4	21	theme	macrophage	675:684	arg1	cells					686:690	RAW264.7 macrophage cells	666:690	RAW264.7 macrophage cells	666:690	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	7	22	theme	delivery	1136:1143	arg1	system					1145:1150	the YGPs-based drug delivery system	1116:1150	the YGPs-based drug delivery system	1116:1150	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	4	23	theme	RAW264.7	666:673	arg1	cells					686:690	RAW264.7 macrophage cells	666:690	RAW264.7 macrophage cells	666:690	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	3	24	theme	used	378:381	arg1	MTX					420:422	MTX	420:422	MTX	420:422	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	24	theme	used	378:381	arg1	methotrexate					406:417	a clinically used anti-inflammatory drug methotrexate	365:417	a clinically used anti-inflammatory drug methotrexate (MTX)	365:423	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	25	theme	yeast	326:330	arg1	particles					339:347	yeast glucan particles	326:347	yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	326:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	25	theme	yeast	326:330	arg1	YGPs					350:353	YGPs	350:353	YGPs	350:353	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	26	from	sites	441:445	arg1	mice					454:457	IBD mice	450:457	IBD mice	450:457	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	1	27	theme	difficult	176:184	arg1	task					186:189	a difficult task	174:189	a difficult task	174:189	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	1	27	theme	difficult	176:184	arg1	Therapy					121:127	Therapy	121:127	Therapy of inflammatory bowel disease (IBD)	121:163	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	3	28	theme	glucan	332:337	arg1	particles					339:347	yeast glucan particles	326:347	yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	326:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	3	28	theme	glucan	332:337	arg1	YGPs					350:353	YGPs	350:353	YGPs	350:353	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	6	29	theme	inflammation	940:951	arg1	site					953:956	the inflammation site	936:956	the inflammation site of colitis mice	936:972	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	6	29	theme	inflammation	940:951	arg1	mice					969:972	colitis mice	961:972	colitis mice	961:972	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	6	30	theme	MTX	1064:1066	arg1	efficacy					1052:1059	the efficacy	1048:1059	the efficacy of MTX	1048:1066	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	4	31	theme	composite	618:626	arg1	YGPs/MTX					628:635	the composite YGPs/MTX	614:635	the composite YGPs/MTX	614:635	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	0	32	theme	Inflammatory	30:41	arg1	Disease					49:55	Inflammatory Bowel Disease	30:55	Inflammatory Bowel Disease	30:55	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.					
32092235	5	33	theme	cells	795:799	arg1	proliferation					767:779	the proliferation	763:779	the proliferation of macrophage cells	763:799	Furthermore, YGPs/MTX can suppress the proliferation of macrophage cells efficiently, leading to down-regulation of pro-inflammatory cytokines induced by lipopolysaccharides.					
32092235	4	34	theme	alginate	595:602	arg1	reaction					583:590	gelation reaction	574:590	gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors	574:725	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	7	35	theme	YGPs-based	1120:1129	arg1	system					1145:1150	the YGPs-based drug delivery system	1116:1150	the YGPs-based drug delivery system	1116:1150	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	3	36	theme	delivery	307:314	arg1	system					316:321	a targeted oral delivery system	291:321	a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	291:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	6	37	theme	inflammatory	1007:1018	arg1	site					1020:1023	the inflammatory site	1003:1023	the inflammatory site	1003:1023	Additionally, YGPs accumulate in the inflammation site of colitis mice, enabling YGPs/MTX to target the inflammatory site, significantly improve the efficacy of MTX, and reduce the cytotoxicity of MTX.					
32092235	3	38	theme	inflamed	432:439	arg1	sites					441:445	the inflamed sites	428:445	the inflamed sites in IBD mice for therapy	428:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	0	39	theme	Bowel	43:47	arg1	Disease					49:55	Inflammatory Bowel Disease	30:55	Inflammatory Bowel Disease	30:55	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.					
32092235	1	40	theme	disease	151:157	arg1	task					186:189	a difficult task	174:189	a difficult task	174:189	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	1	40	theme	disease	151:157	arg1	Therapy					121:127	Therapy	121:127	Therapy of inflammatory bowel disease (IBD)	121:163	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	2	41	theme	clinical	230:237	arg1	need					239:242	a great clinical need	222:242	a great clinical need for more effective treatments for IBD	222:280	There is a great clinical need for more effective treatments for IBD.					
32092235	1	42	from	task	186:189	arg1	field					206:210	the medical field	194:210	the medical field	194:210	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	7	43	theme	IBD	1225:1227	arg1	treatment					1212:1220	the clinical treatment	1199:1220	the clinical treatment of IBD	1199:1227	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	1	44	theme	medical	198:204	arg1	field					206:210	the medical field	194:210	the medical field	194:210	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	2	45	theme	great	224:228	arg1	need					239:242	a great clinical need	222:242	a great clinical need for more effective treatments for IBD	222:280	There is a great clinical need for more effective treatments for IBD.					
32092235	1	46	theme	inflammatory	132:143	arg1	disease					151:157	inflammatory bowel disease	132:157	inflammatory bowel disease (IBD)	132:163	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	1	46	theme	inflammatory	132:143	arg1	IBD					160:162	IBD	160:162	IBD	160:162	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	0	47	theme	Targeting	60:68	arg1	Delivery					70:77	Targeting Delivery	60:77	Targeting Delivery of Methotrexate through Glucan Particles	60:118	A Novel Strategy for Treating Inflammatory Bowel Disease by Targeting Delivery of Methotrexate through Glucan Particles.					
32092235	4	48	theme	gelation	574:581	arg1	reaction					583:590	gelation reaction	574:590	gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors	574:725	In the findings, MTX is effectively loaded into YGPs through re-precipitation followed by gelation reaction of alginate to obtain the composite YGPs/MTX, which are internalized into RAW264.7 macrophage cells through dectin-1 and CR3 receptors.					
32092235	1	49	theme	bowel	145:149	arg1	disease					151:157	inflammatory bowel disease	132:157	inflammatory bowel disease (IBD)	132:163	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	1	49	theme	bowel	145:149	arg1	IBD					160:162	IBD	160:162	IBD	160:162	Therapy of inflammatory bowel disease (IBD) has been a difficult task in the medical field.					
32092235	3	50	theme	targeted	293:300	arg1	system					316:321	a targeted oral delivery system	291:321	a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	291:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
32092235	7	51	theme	drug	1131:1134	arg1	system					1145:1150	the YGPs-based drug delivery system	1116:1150	the YGPs-based drug delivery system	1116:1150	Therefore, the YGPs-based drug delivery system provides a new strategy for MTX application in the clinical treatment of IBD.					
32092235	3	52	theme	particles	339:347	arg1	system					316:321	a targeted oral delivery system	291:321	a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy	291:469	Herein, a targeted oral delivery system of yeast glucan particles (YGPs) carrying a clinically used anti-inflammatory drug methotrexate (MTX) to the inflamed sites in IBD mice for therapy is reported.					
34821899	8	0	theme	secretion	960:968	arg1	proportions					928:938	the proportions	924:938	the proportions of abnormal granule secretion by Paneth cells	924:984	MS increased the proportions of abnormal granule secretion by Paneth cells.					
34821899	7	1	theme	intestinal	778:787	arg1	development					789:799	intestinal development	778:799	intestinal development	778:799	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	7	2	theme	progeny	897:903	arg1	mice					905:908	3-week-old progeny mice	886:908	3-week-old progeny mice	886:908	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	11	3	theme	bacteria	1239:1246	arg1	abundances					1208:1217	The relative abundances	1195:1217	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales	1195:1326	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	14	4	theme	early	1685:1689	arg1	life					1691:1694	early life	1685:1694	early life	1685:1694	Sucralose should be consumed with caution, especially during pregnancy and in early life.					
34821899	1	5	from	syndrome	311:318	arg1	life					329:332	life	329:332	life	329:332	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	4	6	theme	offspring	568:576	arg1	defects					527:533	Paneth cell defects	515:533	Paneth cell defects	515:533	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	4	6	theme	offspring	568:576	arg1	dysbiosis					555:563	exacerbated gut dysbiosis	539:563	exacerbated gut dysbiosis of offspring	539:576	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	0	7	theme	mice	97:100	arg1	dysbiosis					76:84	gut dysbiosis	72:84	gut dysbiosis of progeny mice	72:100	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	1	8	from	disease	289:295	arg1	life					329:332	life	329:332	life	329:332	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	11	9	theme	MS	1364:1365	arg1	group					1367:1371	the MS group	1360:1371	the MS group	1360:1371	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	0	10	theme	progeny	89:95	arg1	mice					97:100	progeny mice	89:100	progeny mice	89:100	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	9	11	theme	MS	1093:1094	arg1	group					1096:1100	the MS group	1089:1100	the MS group	1089:1100	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	10	12	from	composition	1135:1145	arg1	mice					1189:1192	the 3-week-old offspring mice	1164:1192	the 3-week-old offspring mice	1164:1192	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	7	13	theme	small	866:870	arg1	intestines					872:881	the small intestines	862:881	the small intestines of 3-week-old progeny mice	862:908	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	5	14	theme	MS	621:622	arg1	groups					644:649	the MS and control (water) groups	617:649	the MS and control (water) groups	617:649	Female C57BL/6 mice were divided into the MS and control (water) groups during pregnancy and lactation.					
34821899	10	15	theme	3-week-old	1168:1177	arg1	mice					1189:1192	the 3-week-old offspring mice	1164:1192	the 3-week-old offspring mice	1164:1192	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	6	16	theme	Progeny	683:689	arg1	mice					691:694	Progeny mice	683:694	Progeny mice	683:694	Progeny mice were fed a normal sucralose-free diet after weaning until adulthood.					
34821899	0	17	theme	sucralose	9:17	arg1	exposure					19:26	Maternal sucralose exposure	0:26	Maternal sucralose exposure	0:26	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	10	18	from	diversity	1151:1159	arg1	mice					1189:1192	the 3-week-old offspring mice	1164:1192	the 3-week-old offspring mice	1164:1192	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	10	19	theme	gut	1120:1122	arg1	composition					1135:1145	gut microbiota composition	1120:1145	gut microbiota composition	1120:1145	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	13	20	theme	Paneth	1538:1543	arg1	defects					1550:1556	Paneth cell defects	1538:1556	Paneth cell defects	1538:1556	These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice.					
34821899	0	21	theme	Maternal	0:7	arg1	exposure					19:26	Maternal sucralose exposure	0:26	Maternal sucralose exposure	0:26	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	4	22	theme	gut	551:553	arg1	dysbiosis					555:563	exacerbated gut dysbiosis	539:563	exacerbated gut dysbiosis of offspring	539:576	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	5	23	theme	control	628:634	arg1	groups					644:649	the MS and control (water) groups	617:649	the MS and control (water) groups	617:649	Female C57BL/6 mice were divided into the MS and control (water) groups during pregnancy and lactation.					
34821899	5	24	theme	Female	579:584	arg1	mice					594:597	Female C57BL/6 mice	579:597	Female C57BL/6 mice	579:597	Female C57BL/6 mice were divided into the MS and control (water) groups during pregnancy and lactation.					
34821899	11	25	theme	relative	1199:1206	arg1	abundances					1208:1217	The relative abundances	1195:1217	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales	1195:1326	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	1	26	theme	gut	171:173	arg1	microbiota					175:184	the gut microbiota	167:184	the gut microbiota of offspring	167:197	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	1	27	theme	non-alcoholic	263:275	arg1	disease					289:295	non-alcoholic fatty liver disease	263:295	non-alcoholic fatty liver disease	263:295	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	11	28	theme	pro-inflammatory	1222:1237	arg1	Pasteurellales					1291:1304	Pasteurellales	1291:1304	Pasteurellales	1291:1304	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	11	28	theme	pro-inflammatory	1222:1237	arg1	Campylobacterales					1310:1326	Campylobacterales	1310:1326	Campylobacterales	1310:1326	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	11	28	theme	pro-inflammatory	1222:1237	arg1	bacteria					1239:1246	pro-inflammatory bacteria	1222:1246	pro-inflammatory bacteria	1222:1246	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	11	28	theme	pro-inflammatory	1222:1237	arg1	Helicobacter					1277:1288	Helicobacter	1277:1288	Helicobacter	1277:1288	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	5	29	theme	C57BL/6	586:592	arg1	mice					594:597	Female C57BL/6 mice	579:597	Female C57BL/6 mice	579:597	Female C57BL/6 mice were divided into the MS and control (water) groups during pregnancy and lactation.					
34821899	7	30	theme	proinflammatory	833:847	arg1	cytokines					849:857	proinflammatory cytokines	833:857	proinflammatory cytokines	833:857	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	8	31	theme	granule	952:958	arg1	secretion					960:968	abnormal granule secretion	943:968	abnormal granule secretion by Paneth cells	943:984	MS increased the proportions of abnormal granule secretion by Paneth cells.					
34821899	1	32	theme	fatty	277:281	arg1	disease					289:295	non-alcoholic fatty liver disease	263:295	non-alcoholic fatty liver disease	263:295	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	1	33	from	obesity	254:260	arg1	life					329:332	life	329:332	life	329:332	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	5	34	theme	water	637:641	arg1	groups					644:649	the MS and control (water) groups	617:649	the MS and control (water) groups	617:649	Female C57BL/6 mice were divided into the MS and control (water) groups during pregnancy and lactation.					
34821899	1	35	theme	sucralose	136:144	arg1	exposure					151:158	maternal sucralose (MS) exposure	127:158	maternal sucralose (MS) exposure	127:158	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	0	36	theme	cell	43:46	arg1	defects					48:54	Paneth cell defects	36:54	Paneth cell defects	36:54	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	1	37	theme	liver	283:287	arg1	disease					289:295	non-alcoholic fatty liver disease	263:295	non-alcoholic fatty liver disease	263:295	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	3	38	theme	gut	439:441	arg1	microbiota					443:452	the gut microbiota	435:452	the gut microbiota	435:452	Paneth cells are thought to critically influence the gut microbiota.					
34821899	13	39	theme	cell	1545:1548	arg1	defects					1550:1556	Paneth cell defects	1538:1556	Paneth cell defects	1538:1556	These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice.					
34821899	1	40	theme	offspring	189:197	arg1	microbiota					175:184	the gut microbiota	167:184	the gut microbiota of offspring	167:197	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	0	41	theme	Paneth	36:41	arg1	defects					48:54	Paneth cell defects	36:54	Paneth cell defects	36:54	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	4	42	theme	cell	522:525	arg1	defects					527:533	Paneth cell defects	515:533	Paneth cell defects	515:533	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	9	43	theme	Paneth	1001:1006	arg1	cells					1008:1012	Paneth cells	1001:1012	Paneth cells	1001:1012	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	7	44	from	expression	819:828	arg1	intestines					872:881	the small intestines	862:881	the small intestines of 3-week-old progeny mice	862:908	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	13	45	theme	MS	1518:1519	arg1	exposure					1521:1528	MS exposure	1518:1528	MS exposure	1518:1528	These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice.					
34821899	3	46	theme	Paneth	386:391	arg1	cells					393:397	Paneth cells	386:397	Paneth cells	386:397	Paneth cells are thought to critically influence the gut microbiota.					
34821899	4	47	theme	Paneth	515:520	arg1	defects					527:533	Paneth cell defects	515:533	Paneth cell defects	515:533	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	4	48	theme	exacerbated	539:549	arg1	dysbiosis					555:563	exacerbated gut dysbiosis	539:563	exacerbated gut dysbiosis of offspring	539:576	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	13	49	theme	offspring	1591:1599	arg1	mice					1601:1604	offspring mice	1591:1604	offspring mice	1591:1604	These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice.					
34821899	1	50	theme	metabolic	301:309	arg1	syndrome					311:318	metabolic syndrome	301:318	metabolic syndrome	301:318	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	10	51	dep	composition	1135:1145	arg1	the					1116:1118	the	1116:1118	the	1116:1118	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	8	52	theme	abnormal	943:950	arg1	secretion					960:968	abnormal granule secretion	943:968	abnormal granule secretion by Paneth cells	943:984	MS increased the proportions of abnormal granule secretion by Paneth cells.					
34821899	11	53	theme	anti-inflammatory	1380:1396	arg1	bacteria					1398:1405	anti-inflammatory bacteria	1380:1405	anti-inflammatory bacteria	1380:1405	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	11	53	theme	anti-inflammatory	1380:1396	arg1	XI					1430:1431	Clostridium XI	1418:1431	Clostridium XI	1418:1431	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	9	54	theme	mRNA	1018:1021	arg1	expression					1023:1032	mRNA expression	1018:1032	mRNA expression of AMPs such as cryptdins and lysozyme	1018:1071	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	10	55	theme	microbiota	1124:1133	arg1	composition					1135:1145	gut microbiota composition	1120:1145	gut microbiota composition	1120:1145	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	10	56	theme	offspring	1179:1187	arg1	mice					1189:1192	the 3-week-old offspring mice	1164:1192	the 3-week-old offspring mice	1164:1192	MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice.					
34821899	11	57	theme	Clostridium	1418:1428	arg1	XI					1430:1431	Clostridium XI	1418:1431	Clostridium XI	1418:1431	The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased.					
34821899	6	58	theme	sucralose-free	714:727	arg1	diet					729:732	a normal sucralose-free diet	705:732	a normal sucralose-free diet	705:732	Progeny mice were fed a normal sucralose-free diet after weaning until adulthood.					
34821899	1	59	theme	maternal	127:134	arg1	exposure					151:158	maternal sucralose (MS) exposure	127:158	maternal sucralose (MS) exposure	127:158	Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life.					
34821899	7	60	theme	mice	905:908	arg1	intestines					872:881	the small intestines	862:881	the small intestines of 3-week-old progeny mice	862:908	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	7	61	theme	cytokines	849:857	arg1	expression					819:828	the expression	815:828	the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice	815:908	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
34821899	0	62	theme	gut	72:74	arg1	dysbiosis					76:84	gut dysbiosis	72:84	gut dysbiosis of progeny mice	72:100	Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.					
34821899	13	63	theme	exacerbated	1562:1572	arg1	dysbiosis					1578:1586	exacerbated gut dysbiosis	1562:1586	exacerbated gut dysbiosis	1562:1586	These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice.					
34821899	6	64	theme	normal	707:712	arg1	diet					729:732	a normal sucralose-free diet	705:732	a normal sucralose-free diet	705:732	Progeny mice were fed a normal sucralose-free diet after weaning until adulthood.					
34821899	2	65	theme	underlying	348:357	arg1	mechanism					359:367	the underlying mechanism	344:367	the underlying mechanism	344:367	However, the underlying mechanism remains unclear.					
34821899	9	66	theme	AMPs	1037:1040	arg1	lysozyme					1064:1071	lysozyme	1064:1071	lysozyme	1064:1071	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	66	theme	AMPs	1037:1040	arg1	number					991:996	The number	987:996	The number of Paneth cells	987:1012	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	66	theme	AMPs	1037:1040	arg1	cryptdins					1050:1058	cryptdins	1050:1058	cryptdins	1050:1058	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	66	theme	AMPs	1037:1040	arg1	expression					1023:1032	mRNA expression	1018:1032	mRNA expression of AMPs such as cryptdins and lysozyme	1018:1071	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	67	theme	cells	1008:1012	arg1	lysozyme					1064:1071	lysozyme	1064:1071	lysozyme	1064:1071	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	67	theme	cells	1008:1012	arg1	number					991:996	The number	987:996	The number of Paneth cells	987:1012	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	67	theme	cells	1008:1012	arg1	cryptdins					1050:1058	cryptdins	1050:1058	cryptdins	1050:1058	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	9	67	theme	cells	1008:1012	arg1	expression					1023:1032	mRNA expression	1018:1032	mRNA expression of AMPs such as cryptdins and lysozyme	1018:1071	The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group.					
34821899	4	68	theme	MS	495:496	arg1	exposure					498:505	MS exposure	495:505	MS exposure	495:505	This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring.					
34821899	13	69	theme	gut	1574:1576	arg1	dysbiosis					1578:1586	exacerbated gut dysbiosis	1562:1586	exacerbated gut dysbiosis	1562:1586	These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice.					
34821899	8	70	theme	Paneth	973:978	arg1	cells					980:984	Paneth cells	973:984	Paneth cells	973:984	MS increased the proportions of abnormal granule secretion by Paneth cells.					
34821899	7	71	theme	3-week-old	886:895	arg1	mice					905:908	3-week-old progeny mice	886:908	3-week-old progeny mice	886:908	MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice.					
32142843	5	0	from	modulus	718:724	arg1	ratio					759:763	WPI/PSG ratio	751:763	WPI/PSG ratio of 1:1	751:770	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	7	1	theme	composite	878:886	arg1	films					888:892	WPI/PSG composite films	870:892	WPI/PSG composite films	870:892	WPI/PSG composite films were more effective than single films in reducing the surface cracks and degree of cracks.					
32142843	4	2	theme	whiteness	618:626	arg1	index					628:632	higher film brightness and whiteness index	591:632	index	628:632	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	5	3	theme	tensile	689:695	arg1	strength					697:704	tensile strength	689:704	tensile strength	689:704	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	3	theme	tensile	689:695	arg1	modulus					718:724	elastic modulus	710:724	elastic modulus	710:724	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	3	theme	tensile	689:695	arg1	highest					780:786	highest	780:786	highest	780:786	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	4	from	strength	697:704	arg1	ratio					759:763	WPI/PSG ratio	751:763	WPI/PSG ratio of 1:1	751:770	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	1	5	theme	whey	158:161	arg1	WPI					180:182	WPI	180:182	WPI	180:182	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	1	5	theme	whey	158:161	arg1	isolate					171:177	whey protein isolate	158:177	whey protein isolate (WPI)	158:183	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	11	6	theme	highest	1365:1371	arg1	physicochemical					1387:1401	comprehensive physicochemical	1373:1401	comprehensive physicochemical	1373:1401	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	0	7	theme	protein	94:100	arg1	gum					124:126	whey protein isolate/psyllium seed gum	89:126	whey protein isolate/psyllium seed gum	89:126	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	2	8	theme	WPI/PSG	328:334	arg1	ratios					318:323	different ratios	308:323	different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1)	308:360	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	3	9	theme	oxygen	465:470	arg1	permeability					472:483	lower oxygen permeability	459:483	lower oxygen permeability	459:483	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	3	10	theme	light	489:493	arg1	transmittance					495:507	light transmittance	489:507	light transmittance	489:507	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	0	11	theme	whey	89:92	arg1	gum					124:126	whey protein isolate/psyllium seed gum	89:126	whey protein isolate/psyllium seed gum	89:126	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	2	12	theme	mechanical	252:261	arg1	properties					278:287	Its physicochemical, mechanical and structural properties	231:287	Its physicochemical, mechanical and structural properties	231:287	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	11	13	theme	mechanical	1407:1416	arg1	performance					1418:1428	mechanical performance	1407:1428	mechanical performance	1407:1428	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	2	14	dep	WPI/PSG	328:334	arg1	1:1					347:349	1:1	347:349	1:1	347:349	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	2	14	dep	WPI/PSG	328:334	arg1	0:1					357:359	0:1	357:359	0:1	357:359	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	2	14	dep	WPI/PSG	328:334	arg1	3:1					342:344	3:1	342:344	3:1	342:344	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	2	14	dep	WPI/PSG	328:334	arg1	1:0					337:339	1:0	337:339	1:0	337:339	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	2	14	dep	WPI/PSG	328:334	arg1	1:3					352:354	1:3	352:354	1:3	352:354	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	11	15	theme	composite	1310:1318	arg1	film					1320:1323	The composite film	1306:1323	The composite film at WPI/PSG ratio of 1:1	1306:1347	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	5	16	theme	1:1	768:770	arg1	ratio					759:763	WPI/PSG ratio	751:763	WPI/PSG ratio of 1:1	751:770	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	0	17	theme	seed	119:122	arg1	gum					124:126	whey protein isolate/psyllium seed gum	89:126	whey protein isolate/psyllium seed gum	89:126	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	5	18	theme	WPI/PSG	751:757	arg1	ratio					759:763	WPI/PSG ratio	751:763	WPI/PSG ratio of 1:1	751:770	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	11	19	theme	1:1	1345:1347	arg1	ratio					1336:1340	WPI/PSG ratio	1328:1340	WPI/PSG ratio of 1:1	1328:1347	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	1	20	theme	protein	163:169	arg1	WPI					180:182	WPI	180:182	WPI	180:182	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	1	20	theme	protein	163:169	arg1	isolate					171:177	whey protein isolate	158:177	whey protein isolate (WPI)	158:183	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	0	21	theme	isolate/psyllium	102:117	arg1	gum					124:126	whey protein isolate/psyllium seed gum	89:126	whey protein isolate/psyllium seed gum	89:126	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	9	22	theme	peak	1126:1129	arg1	strength					1131:1138	higher diffraction peak strength	1107:1138	higher diffraction peak strength	1107:1138	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	2	23	theme	physicochemical	235:249	arg1	properties					278:287	Its physicochemical, mechanical and structural properties	231:287	Its physicochemical, mechanical and structural properties	231:287	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	10	24	theme	composite	1243:1251	arg1	film					1253:1256	the WPI/PSG composite film	1231:1256	the WPI/PSG composite film	1231:1256	The results indicated that the properties of the WPI/PSG composite film were superior to that of PSG or WPI film alone.					
32142843	4	25	theme	PSG	572:574	arg1	concentration					576:588	PSG concentration	572:588	PSG concentration	572:588	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	9	26	theme	films	1165:1169	arg1	strength					1131:1138	higher diffraction peak strength	1107:1138	higher diffraction peak strength	1107:1138	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	9	26	theme	films	1165:1169	arg1	crystallinity					1144:1156	crystallinity	1144:1156	crystallinity	1144:1156	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	5	27	dep	strength	697:704	arg1	The					685:687	The	685:687	The	685:687	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	9	28	theme	content	1098:1104	arg1	increase					1078:1085	the increase	1074:1085	the increase of the PSG content	1074:1104	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	4	29	theme	color	653:657	arg1	difference					659:668	smaller total color difference	639:668	smaller total color difference	639:668	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	4	30	theme	higher	591:596	arg1	brightness					603:612	higher film brightness and whiteness index	591:632	brightness	603:612	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	0	31	theme	Physicochemical	0:14	arg1	properties					43:52	Physicochemical, mechanical and structural properties	0:52	Physicochemical, mechanical and structural properties of composite edible films	0:78	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	7	32	theme	cracks	977:982	arg1	cracks					956:961	the surface cracks	944:961	the surface cracks	944:961	WPI/PSG composite films were more effective than single films in reducing the surface cracks and degree of cracks.					
32142843	7	32	theme	cracks	977:982	arg1	degree					967:972	degree	967:972	degree of cracks	967:982	WPI/PSG composite films were more effective than single films in reducing the surface cracks and degree of cracks.					
32142843	10	33	theme	WPI	1290:1292	arg1	film					1294:1297	WPI film	1290:1297	WPI film alone	1290:1303	The results indicated that the properties of the WPI/PSG composite film were superior to that of PSG or WPI film alone.					
32142843	4	34	theme	total	647:651	arg1	difference					659:668	smaller total color difference	639:668	smaller total color difference	639:668	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	3	35	theme	WPI/PSG	363:369	arg1	films					381:385	WPI/PSG composite films	363:385	WPI/PSG composite films	363:385	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	5	36	theme	composite	733:741	arg1	film					743:746	the composite film	729:746	the composite film at WPI/PSG ratio of 1:1	729:770	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	9	37	theme	higher	1107:1112	arg1	strength					1131:1138	higher diffraction peak strength	1107:1138	higher diffraction peak strength	1107:1138	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	5	38	theme	elastic	710:716	arg1	strength					697:704	tensile strength	689:704	tensile strength	689:704	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	38	theme	elastic	710:716	arg1	modulus					718:724	elastic modulus	710:724	elastic modulus	710:724	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	38	theme	elastic	710:716	arg1	highest					780:786	highest	780:786	highest	780:786	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	4	39	from	increase	560:567	arg1	concentration					576:588	PSG concentration	572:588	PSG concentration	572:588	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	3	40	theme	water	398:402	arg1	angle					412:416	higher water contact angle and water vapor permeability	391:445	angle	412:416	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	0	41	theme	mechanical	17:26	arg1	properties					43:52	Physicochemical, mechanical and structural properties	0:52	Physicochemical, mechanical and structural properties of composite edible films	0:78	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	6	42	theme	single	856:861	arg1	films					863:867	the single films	852:867	the single films	852:867	Elongation at break of composite films was higher than that of the single films.					
32142843	9	43	theme	diffraction	1114:1124	arg1	strength					1131:1138	higher diffraction peak strength	1107:1138	higher diffraction peak strength	1107:1138	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	2	44	theme	structural	267:276	arg1	properties					278:287	Its physicochemical, mechanical and structural properties	231:287	Its physicochemical, mechanical and structural properties	231:287	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	6	45	theme	films	822:826	arg1	break					803:807	break	803:807	break of composite films	803:826	Elongation at break of composite films was higher than that of the single films.					
32142843	3	46	theme	contact	404:410	arg1	angle					412:416	higher water contact angle and water vapor permeability	391:445	angle	412:416	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	7	47	theme	surface	948:954	arg1	cracks					956:961	the surface cracks	944:961	the surface cracks	944:961	WPI/PSG composite films were more effective than single films in reducing the surface cracks and degree of cracks.					
32142843	3	48	contain	had	387:389	arg2	angle					412:416	higher water contact angle and water vapor permeability	391:445	angle	412:416	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	3	48	contain	had	387:389	arg1	films					381:385	WPI/PSG composite films	363:385	WPI/PSG composite films	363:385	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	3	48	contain	had	387:389	arg2	permeability					472:483	lower oxygen permeability	459:483	lower oxygen permeability	459:483	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	3	48	contain	had	387:389	arg2	permeability					434:445	higher water contact angle and water vapor permeability	391:445	permeability	434:445	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	3	49	theme	single	526:531	arg1	WPI					533:535	single WPI	526:535	single WPI	526:535	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	11	50	theme	WPI/PSG	1328:1334	arg1	ratio					1336:1340	WPI/PSG ratio	1328:1340	WPI/PSG ratio of 1:1	1328:1347	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	1	51	theme	psyllium	189:196	arg1	PSG					208:210	PSG	208:210	PSG	208:210	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	1	51	theme	psyllium	189:196	arg1	gum					203:205	psyllium seed gum	189:205	psyllium seed gum (PSG)	189:211	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	0	52	theme	structural	32:41	arg1	properties					43:52	Physicochemical, mechanical and structural properties	0:52	Physicochemical, mechanical and structural properties of composite edible films	0:78	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	10	53	theme	WPI/PSG	1235:1241	arg1	film					1253:1256	the WPI/PSG composite film	1231:1256	the WPI/PSG composite film	1231:1256	The results indicated that the properties of the WPI/PSG composite film were superior to that of PSG or WPI film alone.					
32142843	3	54	theme	WPI	533:535	arg1	films					544:548	single WPI or PSG films	526:548	single WPI or PSG films	526:548	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	5	55	from	ratio	759:763	arg1	strength					697:704	tensile strength	689:704	tensile strength	689:704	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	55	from	ratio	759:763	arg1	modulus					718:724	elastic modulus	710:724	elastic modulus	710:724	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	55	from	ratio	759:763	arg1	highest					780:786	highest	780:786	highest	780:786	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	55	from	ratio	759:763	arg1	film					743:746	the composite film	729:746	the composite film at WPI/PSG ratio of 1:1	729:770	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	1	56	theme	seed	198:201	arg1	PSG					208:210	PSG	208:210	PSG	208:210	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	1	56	theme	seed	198:201	arg1	gum					203:205	psyllium seed gum	189:205	psyllium seed gum (PSG)	189:211	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	0	57	theme	composite	57:65	arg1	films					74:78	composite edible films	57:78	composite edible films	57:78	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	8	58	theme	films	1062:1066	arg1	structure					1035:1043	a typical semi-crystalline amorphous structure	998:1043	a typical semi-crystalline amorphous structure of the composite films	998:1066	XRD revealed a typical semi-crystalline amorphous structure of the composite films.					
32142843	3	59	theme	composite	371:379	arg1	films					381:385	WPI/PSG composite films	363:385	WPI/PSG composite films	363:385	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	11	60	from	ratio	1336:1340	arg1	film					1320:1323	The composite film	1306:1323	The composite film at WPI/PSG ratio of 1:1	1306:1347	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	7	61	theme	single	919:924	arg1	films					926:930	single films	919:930	single films	919:930	WPI/PSG composite films were more effective than single films in reducing the surface cracks and degree of cracks.					
32142843	3	62	theme	water	422:426	arg1	permeability					434:445	higher water contact angle and water vapor permeability	391:445	permeability	434:445	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	6	63	theme	composite	812:820	arg1	films					822:826	composite films	812:826	composite films	812:826	Elongation at break of composite films was higher than that of the single films.					
32142843	3	64	theme	vapor	428:432	arg1	permeability					434:445	higher water contact angle and water vapor permeability	391:445	permeability	434:445	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	8	65	theme	semi-crystalline	1008:1023	arg1	structure					1035:1043	a typical semi-crystalline amorphous structure	998:1043	a typical semi-crystalline amorphous structure of the composite films	998:1066	XRD revealed a typical semi-crystalline amorphous structure of the composite films.					
32142843	9	66	theme	PSG	1094:1096	arg1	content					1098:1104	the PSG content	1090:1104	the PSG content	1090:1104	With the increase of the PSG content, higher diffraction peak strength and crystallinity of the films were observed.					
32142843	6	67	from	break	803:807	arg1	Elongation					789:798	Elongation	789:798	Elongation at break of composite films	789:826	Elongation at break of composite films was higher than that of the single films.					
32142843	8	68	theme	typical	1000:1006	arg1	structure					1035:1043	a typical semi-crystalline amorphous structure	998:1043	a typical semi-crystalline amorphous structure of the composite films	998:1066	XRD revealed a typical semi-crystalline amorphous structure of the composite films.					
32142843	2	69	theme	different	308:316	arg1	ratios					318:323	different ratios	308:323	different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1)	308:360	Its physicochemical, mechanical and structural properties were determined at different ratios of WPI/PSG (1:0, 3:1, 1:1, 1:3, 0:1).					
32142843	10	70	theme	film	1253:1256	arg1	properties					1217:1226	the properties	1213:1226	the properties of the WPI/PSG composite film	1213:1256	The results indicated that the properties of the WPI/PSG composite film were superior to that of PSG or WPI film alone.					
32142843	10	70	theme	film	1253:1256	arg1	superior					1263:1270	superior	1263:1270	superior	1263:1270	The results indicated that the properties of the WPI/PSG composite film were superior to that of PSG or WPI film alone.					
32142843	3	71	theme	lower	459:463	arg1	permeability					472:483	lower oxygen permeability	459:483	lower oxygen permeability	459:483	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	5	72	theme	film	743:746	arg1	strength					697:704	tensile strength	689:704	tensile strength	689:704	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	72	theme	film	743:746	arg1	modulus					718:724	elastic modulus	710:724	elastic modulus	710:724	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	5	72	theme	film	743:746	arg1	highest					780:786	highest	780:786	highest	780:786	The tensile strength and elastic modulus of the composite film at WPI/PSG ratio of 1:1 was the highest.					
32142843	1	73	theme	composite	131:139	arg1	film					141:144	A composite film	129:144	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG)	129:211	A composite film composed of whey protein isolate (WPI) and psyllium seed gum (PSG) was investigated.					
32142843	8	74	theme	amorphous	1025:1033	arg1	structure					1035:1043	a typical semi-crystalline amorphous structure	998:1043	a typical semi-crystalline amorphous structure of the composite films	998:1066	XRD revealed a typical semi-crystalline amorphous structure of the composite films.					
32142843	4	75	theme	smaller	639:645	arg1	difference					659:668	smaller total color difference	639:668	smaller total color difference	639:668	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	0	76	theme	films	74:78	arg1	properties					43:52	Physicochemical, mechanical and structural properties	0:52	Physicochemical, mechanical and structural properties of composite edible films	0:78	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	3	77	theme	higher	391:396	arg1	angle					412:416	higher water contact angle and water vapor permeability	391:445	angle	412:416	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	11	78	theme	comprehensive	1373:1385	arg1	physicochemical					1387:1401	comprehensive physicochemical	1373:1401	comprehensive physicochemical	1373:1401	The composite film at WPI/PSG ratio of 1:1 resulted in the highest comprehensive physicochemical and mechanical performance.					
32142843	0	79	theme	edible	67:72	arg1	films					74:78	composite edible films	57:78	composite edible films	57:78	Physicochemical, mechanical and structural properties of composite edible films based on whey protein isolate/psyllium seed gum.					
32142843	3	80	theme	PSG	540:542	arg1	films					544:548	single WPI or PSG films	526:548	single WPI or PSG films	526:548	WPI/PSG composite films had higher water contact angle and water vapor permeability, as well as lower oxygen permeability and light transmittance as compared with single WPI or PSG films.					
32142843	7	81	theme	WPI/PSG	870:876	arg1	films					888:892	WPI/PSG composite films	870:892	WPI/PSG composite films	870:892	WPI/PSG composite films were more effective than single films in reducing the surface cracks and degree of cracks.					
32142843	4	82	theme	film	598:601	arg1	brightness					603:612	higher film brightness and whiteness index	591:632	brightness	603:612	With the increase in PSG concentration, higher film brightness and whiteness index, and smaller total color difference were observed.					
32142843	8	83	theme	composite	1052:1060	arg1	films					1062:1066	the composite films	1048:1066	the composite films	1048:1066	XRD revealed a typical semi-crystalline amorphous structure of the composite films.					
33076773	3	0	theme	CS/CS-β-TCP	344:354	arg1	scaffold					356:363	The bi-layered CS/CS-β-TCP scaffold	329:363	Approach: The bi-layered CS/CS-β-TCP scaffold	319:363	Approach: The bi-layered CS/CS-β-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope.					
33076773	11	1	theme	scaffolds	1438:1446	arg1	group					1448:1452	the bi-layered scaffolds group	1423:1452	the bi-layered scaffolds group	1423:1452	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	6	2	theme	layer	746:750	arg1	ability					767:773	osteogenic ability	756:773	osteogenic ability of the CS-β-TCP layer	756:795	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	6	2	theme	layer	746:750	arg1	ability					728:734	the chondrogenic ability	711:734	the chondrogenic ability of the CS layer	711:750	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	7	3	theme	harvested	924:932	arg1	samples					934:940	the harvested samples	920:940	the harvested samples	920:940	Then, the bi-layered scaffolds were implanted into the rat osteochondral defects and the harvested samples were macroscopically and histologically evaluated.					
33076773	13	4	theme	osteochondral	1965:1977	arg1	engineering					1986:1996	osteochondral tissue engineering	1965:1996	osteochondral tissue engineering	1965:1996	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	4	5	theme	CS	539:540	arg1	layer					542:546	the CS layer	535:546	the CS layer	535:546	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	11	6	dep	in	1380:1381	arg1	vivo					1383:1386	vivo	1383:1386	vivo	1383:1386	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	13	7	theme	tissue	1979:1984	arg1	engineering					1986:1996	osteochondral tissue engineering	1965:1996	osteochondral tissue engineering	1965:1996	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	5	8	dep	Viability	586:594	arg1	ability					614:620	ability	614:620	ability	614:620	Viability and proliferation ability of the cells were observed under a confocal microscope.					
33076773	2	9	dep	aimed	163:167	arg1	constructing					172:183	constructing	172:183	constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	172:270	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	2	9	dep	aimed	163:167	arg1	repairing					279:287	repairing	279:287	repairing the rat osteochondral defect	279:316	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	3	10	dep	Approach	319:326	arg1	scaffold					356:363	The bi-layered CS/CS-β-TCP scaffold	329:363	Approach: The bi-layered CS/CS-β-TCP scaffold	319:363	Approach: The bi-layered CS/CS-β-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope.					
33076773	12	11	theme	bi-layered	1708:1717	arg1	scaffold					1741:1748	the bi-layered composite CS/CS-β-TCP scaffold	1704:1748	the bi-layered composite CS/CS-β-TCP scaffold	1704:1748	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	8	12	theme	distinctive	1052:1062	arg1	microstructures					1064:1078	the distinctive microstructures	1048:1078	the distinctive microstructures for each layer	1048:1093	Results: The bi-layered CS/CS-β-TCP scaffold exhibited the distinctive microstructures for each layer.					
33076773	13	13	theme	osteochondral	1895:1907	arg1	repair					1916:1921	osteochondral defect repair	1895:1921	osteochondral defect repair	1895:1921	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	8	14	theme	bi-layered	1006:1015	arg1	scaffold					1029:1036	The bi-layered CS/CS-β-TCP scaffold	1002:1036	The bi-layered CS/CS-β-TCP scaffold	1002:1036	Results: The bi-layered CS/CS-β-TCP scaffold exhibited the distinctive microstructures for each layer.					
33076773	11	15	theme	osteochondral	1483:1495	arg1	tissues					1497:1503	the native osteochondral tissues	1472:1503	the native osteochondral tissues	1472:1503	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	11	16	theme	repair	1579:1584	arg1	effects					1586:1592	better repair effects	1572:1592	better repair effects	1572:1592	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	12	17	dep	time	1694:1697	arg1	evaluated					1774:1782	evaluated	1774:1782	evaluated with respect to osteochondral defect repair	1774:1826	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	12	17	dep	time	1694:1697	arg1	fabricated					1759:1768	fabricated	1759:1768	fabricated	1759:1768	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	5	18	theme	cells	629:633	arg1	proliferation					600:612	proliferation	600:612	proliferation	600:612	Viability and proliferation ability of the cells were observed under a confocal microscope.					
33076773	5	18	theme	cells	629:633	arg1	Viability					586:594	Viability	586:594	Viability	586:594	Viability and proliferation ability of the cells were observed under a confocal microscope.					
33076773	11	19	theme	hyaline-like	1522:1533	arg1	cartilage					1535:1543	hyaline-like cartilage	1522:1543	hyaline-like cartilage	1522:1543	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	6	20	theme	CS	743:744	arg1	layer					746:750	the CS layer	739:750	the CS layer	739:750	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	10	21	theme	proliferation	1349:1361	arg1	ability					1363:1369	excellent proliferation ability	1339:1369	excellent proliferation ability	1339:1369	Moreover, cells in both layers could maintain well viability and excellent proliferation ability.					
33076773	7	22	theme	rat	890:892	arg1	defects					908:914	the rat osteochondral defects	886:914	the rat osteochondral defects	886:914	Then, the bi-layered scaffolds were implanted into the rat osteochondral defects and the harvested samples were macroscopically and histologically evaluated.					
33076773	12	23	theme	first	1688:1692	arg1	time					1694:1697	the first time	1684:1697	the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair	1684:1826	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	12	23	theme	first	1688:1692	arg1	This					1676:1679	This	1676:1679	This	1676:1679	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	12	23	theme	first	1688:1692	arg1	Innovation					1664:1673	Innovation	1664:1673	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.	1664:1827	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	2	24	theme	osteochondral	297:309	arg1	defect					311:316	the rat osteochondral defect	289:316	the rat osteochondral defect	289:316	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	7	25	theme	osteochondral	894:906	arg1	defects					908:914	the rat osteochondral defects	886:914	the rat osteochondral defects	886:914	Then, the bi-layered scaffolds were implanted into the rat osteochondral defects and the harvested samples were macroscopically and histologically evaluated.					
33076773	12	26	theme	osteochondral	1800:1812	arg1	repair					1821:1826	osteochondral defect repair	1800:1826	osteochondral defect repair	1800:1826	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	2	27	theme	rat	293:295	arg1	defect					311:316	the rat osteochondral defect	289:316	the rat osteochondral defect	289:316	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	12	28	theme	CS/CS-β-TCP	1729:1739	arg1	scaffold					1741:1748	the bi-layered composite CS/CS-β-TCP scaffold	1704:1748	the bi-layered composite CS/CS-β-TCP scaffold	1704:1748	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	0	29	theme	Composite	11:19	arg1	Scaffold					21:28	Bi-layered Composite Scaffold	0:28	Bi-layered Composite Scaffold for Repair of the Osteochondral Defects	0:68	Bi-layered Composite Scaffold for Repair of the Osteochondral Defects.					
33076773	11	30	theme	in	1380:1381	arg1	study					1388:1392	the in vivo study	1376:1392	the in vivo study	1376:1392	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	1	31	theme	Osteochondral	82:94	arg1	defect					96:101	Osteochondral defect	82:101	Osteochondral defect	82:101	Objective: Osteochondral defect presents a big challenge for clinical treatment.					
33076773	9	32	theme	chondrogenic	1155:1166	arg1	lineage					1168:1174	the chondrogenic lineage	1151:1174	the chondrogenic lineage	1151:1174	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	0	33	theme	Bi-layered	0:9	arg1	Scaffold					21:28	Bi-layered Composite Scaffold	0:28	Bi-layered Composite Scaffold for Repair of the Osteochondral Defects	0:68	Bi-layered Composite Scaffold for Repair of the Osteochondral Defects.					
33076773	5	34	theme	confocal	657:664	arg1	microscope					666:675	a confocal microscope	655:675	a confocal microscope	655:675	Viability and proliferation ability of the cells were observed under a confocal microscope.					
33076773	11	35	theme	formed	1405:1410	arg1	similar					1459:1465	similar	1459:1465	similar	1459:1465	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	11	35	theme	formed	1405:1410	arg1	tissues					1412:1418	the newly formed tissues	1395:1418	the newly formed tissues in the bi-layered scaffolds group	1395:1452	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	9	36	from	chondrocytes	1107:1118	arg1	layer					1130:1134	the CS layer	1123:1134	the CS layer	1123:1134	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	6	37	theme	chondrogenic	715:726	arg1	ability					728:734	the chondrogenic ability	711:734	the chondrogenic ability of the CS layer	711:750	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	11	38	theme	CS	1626:1627	arg1	group					1629:1633	the pure CS group	1617:1633	the pure CS group	1617:1633	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	12	39	theme	defect	1814:1819	arg1	repair					1821:1826	osteochondral defect repair	1800:1826	osteochondral defect repair	1800:1826	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	11	40	theme	subchondral	1549:1559	arg1	bone					1561:1564	subchondral bone	1549:1564	subchondral bone	1549:1564	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	11	41	theme	blank	1643:1647	arg1	group					1657:1661	the blank control group	1639:1661	the blank control group	1639:1661	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	4	42	theme	marrow	492:497	arg1	BMSCs					511:515	BMSCs	511:515	BMSCs	511:515	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	4	42	theme	marrow	492:497	arg1	cells					504:508	bone marrow stem cells	487:508	bone marrow stem cells (BMSCs)	487:516	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	7	43	theme	bi-layered	845:854	arg1	scaffolds					856:864	the bi-layered scaffolds	841:864	the bi-layered scaffolds	841:864	Then, the bi-layered scaffolds were implanted into the rat osteochondral defects and the harvested samples were macroscopically and histologically evaluated.					
33076773	12	44	theme	composite	1719:1727	arg1	scaffold					1741:1748	the bi-layered composite CS/CS-β-TCP scaffold	1704:1748	the bi-layered composite CS/CS-β-TCP scaffold	1704:1748	Innovation: This is the first time that the bi-layered composite CS/CS-β-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair.					
33076773	3	45	theme	scanning	440:447	arg1	microscope					458:467	a scanning electron microscope	438:467	a scanning electron microscope	438:467	Approach: The bi-layered CS/CS-β-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope.					
33076773	4	46	theme	bone	487:490	arg1	marrow					492:497	bone marrow	487:497	bone marrow stem cells (BMSCs)	487:516	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	9	47	theme	CS	1127:1128	arg1	layer					1130:1134	the CS layer	1123:1134	the CS layer	1123:1134	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	11	48	theme	pure	1621:1624	arg1	group					1629:1633	the pure CS group	1617:1633	the pure CS group	1617:1633	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	3	49	theme	electron	449:456	arg1	microscope					458:467	a scanning electron microscope	438:467	a scanning electron microscope	438:467	Approach: The bi-layered CS/CS-β-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope.					
33076773	11	50	theme	bi-layered	1427:1436	arg1	group					1448:1452	the bi-layered scaffolds group	1423:1452	the bi-layered scaffolds group	1423:1452	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	9	51	from	BMSCs	1185:1189	arg1	layer					1207:1211	the CS-β-TCP layer	1194:1211	the CS-β-TCP layer	1194:1211	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	6	52	theme	layer	791:795	arg1	ability					767:773	osteogenic ability	756:773	osteogenic ability of the CS-β-TCP layer	756:795	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	6	52	theme	layer	791:795	arg1	ability					728:734	the chondrogenic ability	711:734	the chondrogenic ability of the CS layer	711:750	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	0	53	theme	Osteochondral	48:60	arg1	Defects					62:68	the Osteochondral Defects	44:68	the Osteochondral Defects	44:68	Bi-layered Composite Scaffold for Repair of the Osteochondral Defects.					
33076773	11	54	theme	native	1476:1481	arg1	tissues					1497:1503	the native osteochondral tissues	1472:1503	the native osteochondral tissues	1472:1503	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	4	55	theme	stem	499:502	arg1	BMSCs					511:515	BMSCs	511:515	BMSCs	511:515	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	4	55	theme	stem	499:502	arg1	cells					504:508	bone marrow stem cells	487:508	bone marrow stem cells (BMSCs)	487:516	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	13	56	theme	defect	1909:1914	arg1	repair					1916:1921	osteochondral defect repair	1895:1921	osteochondral defect repair	1895:1921	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	6	57	theme	CS-β-TCP	782:789	arg1	layer					791:795	the CS-β-TCP layer	778:795	the CS-β-TCP layer	778:795	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	1	58	theme	big	114:116	arg1	challenge					118:126	a big challenge	112:126	a big challenge for clinical treatment	112:149	Objective: Osteochondral defect presents a big challenge for clinical treatment.					
33076773	4	59	theme	CS-β-TCP	556:563	arg1	layer					565:569	the CS-β-TCP layer	552:569	the CS-β-TCP layer	552:569	Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-β-TCP layer, respectively.					
33076773	3	60	theme	bi-layered	333:342	arg1	scaffold					356:363	The bi-layered CS/CS-β-TCP scaffold	329:363	Approach: The bi-layered CS/CS-β-TCP scaffold	319:363	Approach: The bi-layered CS/CS-β-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope.					
33076773	2	61	theme	phosphate	239:247	arg1	scaffold					263:270	a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	185:270	a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	185:270	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	9	62	theme	CS-β-TCP	1198:1205	arg1	layer					1207:1211	the CS-β-TCP layer	1194:1211	the CS-β-TCP layer	1194:1211	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	6	63	theme	subcutaneous	684:695	arg1	implantation					697:708	subcutaneous implantation	684:708	subcutaneous implantation	684:708	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	10	64	from	cells	1284:1288	arg1	layers					1298:1303	both layers	1293:1303	both layers	1293:1303	Moreover, cells in both layers could maintain well viability and excellent proliferation ability.					
33076773	2	65	theme	chitosan/chitosan-β-tricalcium	208:237	arg1	CS/CS-β-TCP					250:260	CS/CS-β-TCP	250:260	CS/CS-β-TCP	250:260	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	2	65	theme	chitosan/chitosan-β-tricalcium	208:237	arg1	phosphate					239:247	composite chitosan/chitosan-β-tricalcium phosphate	198:247	a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	185:270	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	9	66	theme	osteogenic	1254:1263	arg1	lineage					1265:1271	the osteogenic lineage	1250:1271	the osteogenic lineage	1250:1271	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	13	67	theme	bi-layered	1845:1854	arg1	scaffolds					1868:1876	The bi-layered CS/CS-β-TCP scaffolds	1841:1876	The bi-layered CS/CS-β-TCP scaffolds	1841:1876	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	11	68	theme	better	1572:1577	arg1	effects					1586:1592	better repair effects	1572:1592	better repair effects	1572:1592	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	9	69	dep	maintain	1142:1149	arg1	whereas					1177:1183	whereas	1177:1183	whereas	1177:1183	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	2	70	theme	composite	198:206	arg1	CS/CS-β-TCP					250:260	CS/CS-β-TCP	250:260	CS/CS-β-TCP	250:260	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	2	70	theme	composite	198:206	arg1	phosphate					239:247	composite chitosan/chitosan-β-tricalcium phosphate	198:247	a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	185:270	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	13	71	theme	promising	1940:1948	arg1	candidates					1950:1959	the promising candidates	1936:1959	the promising candidates for osteochondral tissue engineering	1936:1996	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	13	71	theme	promising	1940:1948	arg1	Conclusion					1829:1838	Conclusion	1829:1838	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.	1829:1997	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
33076773	1	72	theme	clinical	132:139	arg1	treatment					141:149	clinical treatment	132:149	clinical treatment	132:149	Objective: Osteochondral defect presents a big challenge for clinical treatment.					
33076773	9	73	theme	seeded	1100:1105	arg1	chondrocytes					1107:1118	The seeded chondrocytes	1096:1118	The seeded chondrocytes in the CS layer	1096:1134	The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-β-TCP layer could continually differentiate into the osteogenic lineage.					
33076773	2	74	theme	bi-layered	187:196	arg1	scaffold					263:270	a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	185:270	a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold	185:270	This study aimed at constructing a bi-layered composite chitosan/chitosan-β-tricalcium phosphate (CS/CS-β-TCP) scaffold and at repairing the rat osteochondral defect.					
33076773	6	75	theme	osteogenic	756:765	arg1	ability					767:773	osteogenic ability	756:773	osteogenic ability of the CS-β-TCP layer	756:795	After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-β-TCP layer were evaluated by immunofluorescence.					
33076773	11	76	theme	control	1649:1655	arg1	group					1657:1661	the blank control group	1639:1661	the blank control group	1639:1661	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	0	77	theme	Defects	62:68	arg1	Repair					34:39	Repair	34:39	Repair of the Osteochondral Defects	34:68	Bi-layered Composite Scaffold for Repair of the Osteochondral Defects.					
33076773	11	78	from	tissues	1412:1418	arg1	group					1448:1452	the bi-layered scaffolds group	1423:1452	the bi-layered scaffolds group	1423:1452	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	11	79	with	similar	1459:1465	arg1	tissues					1497:1503	the native osteochondral tissues	1472:1503	the native osteochondral tissues	1472:1503	For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group.					
33076773	8	80	theme	CS/CS-β-TCP	1017:1027	arg1	scaffold					1029:1036	The bi-layered CS/CS-β-TCP scaffold	1002:1036	The bi-layered CS/CS-β-TCP scaffold	1002:1036	Results: The bi-layered CS/CS-β-TCP scaffold exhibited the distinctive microstructures for each layer.					
33076773	10	81	theme	excellent	1339:1347	arg1	ability					1363:1369	excellent proliferation ability	1339:1369	excellent proliferation ability	1339:1369	Moreover, cells in both layers could maintain well viability and excellent proliferation ability.					
33076773	13	82	theme	CS/CS-β-TCP	1856:1866	arg1	scaffolds					1868:1876	The bi-layered CS/CS-β-TCP scaffolds	1841:1876	The bi-layered CS/CS-β-TCP scaffolds	1841:1876	Conclusion: The bi-layered CS/CS-β-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.					
32445515	6	0	theme	Knee	853:856	arg1	KOOS					900:903	KOOS	900:903	KOOS	900:903	Knee Injury and Osteoarthritis Outcome Scores (KOOS) were assessed for all subjects, and Kellgren-Lawrence grading was performed for OA volunteers.					
32445515	6	0	theme	Knee	853:856	arg1	Injury					858:863	Knee Injury	853:863	Knee Injury	853:863	Knee Injury and Osteoarthritis Outcome Scores (KOOS) were assessed for all subjects, and Kellgren-Lawrence grading was performed for OA volunteers.					
32445515	7	1	theme	gagCEST	1152:1158	arg1	asymmetry					1160:1168	gagCEST asymmetry and T2 and T1ρ relaxation times	1152:1200	asymmetry	1160:1168	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	3	2	theme	higher	473:478	arg1	asymmetry					488:496	higher gagCEST asymmetry	473:496	higher gagCEST asymmetry	473:496	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	10	3	theme	average	1492:1498	arg1	asymmetry					1508:1516	average gagCEST asymmetry	1492:1516	average gagCEST asymmetry	1492:1516	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	11	4	theme	healthy	1722:1728	arg1	controls					1730:1737	healthy controls	1722:1737	healthy controls	1722:1737	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	12	5	theme	gagCEST	1848:1854	arg1	technique					1856:1864	The gagCEST technique	1844:1864	The gagCEST technique	1844:1864	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	5	6	theme	medial	814:819	arg1	OA					821:822	medial OA	814:822	medial OA	814:822	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	10	7	theme	younger	1526:1532	arg1	p					1562:1562	p = 0.655	1562:1570	p = 0.655	1562:1570	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	7	theme	younger	1526:1532	arg1	controls					1552:1559	younger and older healthy controls	1526:1559	younger and older healthy controls (p = 0.655)	1526:1571	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	3	8	with	sequence	459:466	arg1	asymmetry					488:496	higher gagCEST asymmetry	473:496	higher gagCEST asymmetry	473:496	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	3	9	with	comparison	594:603	arg1	T2					610:611	T2	610:611	T2	610:611	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	3	9	with	comparison	594:603	arg1	T1ρ					617:619	T1ρ	617:619	T1ρ	617:619	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	1	10	theme	glycosaminoglycans	181:198	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	10	theme	glycosaminoglycans	181:198	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	12	11	theme	gagCEST	1931:1937	arg1	asymmetry					1939:1947	high gagCEST asymmetry	1926:1947	high gagCEST asymmetry	1926:1947	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	0	12	theme	range	100:104	arg1	evaluation					69:78	evaluation	69:78	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	7	13	from	T.	1120:1121	arg1	sequence					1106:1113	the volumetric gagCEST sequence	1083:1113	the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity	1083:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	7	13	from	T.	1120:1121	arg1	repeatability					1066:1078	the repeatability	1062:1078	the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity	1062:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	3	14	theme	knee	562:565	arg1	cartilage					577:585	knee articular cartilage	562:585	knee articular cartilage	562:585	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	9	15	theme	average	1396:1402	arg1	%					1448:1448	6.8%	1445:1448	6.8%	1445:1448	The average scan-rescan coefficient of variation was 6.8%.					
32445515	9	15	theme	average	1396:1402	arg1	coefficient					1416:1426	The average scan-rescan coefficient	1392:1426	The average scan-rescan coefficient of variation	1392:1439	The average scan-rescan coefficient of variation was 6.8%.					
32445515	6	16	theme	Outcome	884:890	arg1	Scores					892:897	Osteoarthritis Outcome Scores	869:897	Osteoarthritis Outcome Scores	869:897	Knee Injury and Osteoarthritis Outcome Scores (KOOS) were assessed for all subjects, and Kellgren-Lawrence grading was performed for OA volunteers.					
32445515	8	17	theme	=	1382:1382	arg1	0.015					1384:1388	p = 0.015	1380:1388	p = 0.015	1380:1388	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	8	17	theme	=	1382:1382	arg1	acquisitions					1366:1377	single-slice acquisitions	1353:1377	single-slice acquisitions (p = 0.015)	1353:1389	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	10	18	theme	older	1538:1542	arg1	p					1562:1562	p = 0.655	1562:1570	p = 0.655	1562:1570	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	18	theme	older	1538:1542	arg1	controls					1552:1559	younger and older healthy controls	1526:1559	younger and older healthy controls (p = 0.655)	1526:1571	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	11	19	theme	p	1688:1688	arg1	<					1690:1690	p < 0.001	1688:1696	p < 0.001	1688:1696	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	0	20	theme	population	128:137	arg1	evaluation					69:78	evaluation	69:78	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	9	21	theme	variation	1431:1439	arg1	%					1448:1448	6.8%	1445:1448	6.8%	1445:1448	The average scan-rescan coefficient of variation was 6.8%.					
32445515	9	21	theme	variation	1431:1439	arg1	coefficient					1416:1426	The average scan-rescan coefficient	1392:1426	The average scan-rescan coefficient of variation	1392:1439	The average scan-rescan coefficient of variation was 6.8%.					
32445515	6	22	theme	Kellgren-Lawrence	942:958	arg1	grading					960:966	Kellgren-Lawrence grading	942:966	Kellgren-Lawrence grading	942:966	Knee Injury and Osteoarthritis Outcome Scores (KOOS) were assessed for all subjects, and Kellgren-Lawrence grading was performed for OA volunteers.					
32445515	12	23	theme	volumetric	1887:1896	arg1	times					1903:1907	volumetric scan times	1887:1907	volumetric scan times under 10 min and high gagCEST asymmetry at 3 T	1887:1954	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	5	24	theme	healthy	776:782	arg1	subjects					784:791	10 healthy subjects	773:791	10 healthy subjects	773:791	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	7	25	from	repeatability	1066:1078	arg1	T.					1120:1121	T.	1120:1121	T.	1120:1121	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	7	26	theme	OA	1139:1140	arg1	subjects					1142:1149	healthy and OA subjects	1127:1149	healthy and OA subjects	1127:1149	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	11	27	dep	subjects	1678:1685	arg1	<					1690:1690	p < 0.001	1688:1696	p < 0.001	1688:1696	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	1	28	theme	exchange	149:156	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	28	theme	exchange	149:156	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	11	29	theme	relaxation	1641:1650	arg1	times					1652:1656	T2 and T1ρ relaxation times	1630:1656	times	1652:1656	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	7	30	theme	healthy	1127:1133	arg1	subjects					1142:1149	healthy and OA subjects	1127:1149	healthy and OA subjects	1127:1149	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	12	31	from	T	1954:1954	arg1	asymmetry					1939:1947	high gagCEST asymmetry	1926:1947	high gagCEST asymmetry	1926:1947	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	12	31	from	T	1954:1954	arg1	min					1918:1920	10 min	1915:1920	10 min	1915:1920	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	0	32	theme	improved	83:90	arg1	range					100:104	improved dynamic range	83:104	improved dynamic range	83:104	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	11	33	theme	T1ρ	1637:1639	arg1	times					1652:1656	T2 and T1ρ relaxation times	1630:1656	times	1652:1656	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	12	34	theme	mild-moderate	2024:2036	arg1	OA					2038:2039	mild-moderate OA	2024:2039	mild-moderate OA	2024:2039	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	10	35	theme	=	1564:1564	arg1	p					1562:1562	p = 0.655	1562:1570	p = 0.655	1562:1570	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	35	theme	=	1564:1564	arg1	controls					1552:1559	younger and older healthy controls	1526:1559	younger and older healthy controls (p = 0.655)	1526:1571	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	0	36	theme	Rapid	0:4	arg1	imaging					25:31	Rapid volumetric gagCEST imaging	0:31	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	7	37	theme	articular	1234:1242	arg1	cartilage					1244:1252	the femoral articular cartilage	1222:1252	the femoral articular cartilage	1222:1252	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	1	38	contain	has	246:248	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	38	contain	has	246:248	arg2	potential					250:258	potential	250:258	potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	250:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	38	contain	has	246:248	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	8	39	theme	gagCEST	1303:1309	arg1	imaging					1311:1317	Volumetric gagCEST imaging	1292:1317	Volumetric gagCEST imaging	1292:1317	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	3	40	from	changes	551:557	arg1	cartilage					577:585	knee articular cartilage	562:585	knee articular cartilage	562:585	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	3	40	from	changes	551:557	arg1	comparison					594:603	comparison	594:603	comparison with T2 and T1ρ measures	594:628	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	0	41	theme	gagCEST	17:23	arg1	imaging					25:31	Rapid volumetric gagCEST imaging	0:31	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	1	42	theme	proteoglycan	284:295	arg1	content					297:303	cartilage proteoglycan content	274:303	cartilage proteoglycan content	274:303	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	5	43	with	subjects	784:791	arg1	OA					821:822	medial OA	814:822	medial OA	814:822	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	11	44	theme	total	1780:1784	arg1	scores					1791:1796	total KOOS scores	1780:1796	total KOOS scores (rho = -0.181 and rho = -0.332 respectively)	1780:1841	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	13	45	theme	changes	2133:2139	arg1	assessment					2113:2122	assessment	2113:2122	assessment of early changes in cartilage composition in OA	2113:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	8	46	theme	gagCEST	1330:1336	arg1	asymmetry					1338:1346	higher gagCEST asymmetry	1323:1346	higher gagCEST asymmetry	1323:1346	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	3	47	theme	gagCEST	451:457	arg1	sequence					459:466	a rapid volumetric gagCEST sequence	432:466	a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T	432:503	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	0	48	theme	articular	41:49	arg1	cartilage					51:59	knee articular cartilage	36:59	knee articular cartilage	36:59	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	2	49	from	T	369:369	arg1	utility					356:362	its utility	352:362	its utility at 3 T	352:369	However, its utility at 3 T remains unclear.					
32445515	12	50	theme	healthy	1992:1998	arg1	subjects					2000:2007	healthy subjects	1992:2007	healthy subjects	1992:2007	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	3	51	theme	rapid	434:438	arg1	sequence					459:466	a rapid volumetric gagCEST sequence	432:466	a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T	432:503	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	4	52	from	T	674:674	arg1	asymmetry					659:667	gagCEST asymmetry	651:667	gagCEST asymmetry at 3 T	651:674	We hypothesize that gagCEST asymmetry at 3 T decreases with increasing severity of osteoarthritis (OA).					
32445515	7	53	theme	T1ρ	1181:1183	arg1	times					1196:1200	gagCEST asymmetry and T2 and T1ρ relaxation times	1152:1200	times	1196:1200	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	10	54	theme	significant	1465:1475	arg1	differences					1477:1487	no significant differences	1462:1487	no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310)	1462:1627	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	13	55	from	composition	2154:2164	arg1	assessment					2113:2122	assessment	2113:2122	assessment of early changes in cartilage composition in OA	2113:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	13	55	from	composition	2154:2164	arg1	OA					2169:2170	OA	2169:2170	OA	2169:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	3	56	theme	gagCEST	480:486	arg1	asymmetry					488:496	higher gagCEST asymmetry	473:496	higher gagCEST asymmetry	473:496	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	3	57	dep	T2	610:611	arg1	measures					621:628	measures	621:628	measures	621:628	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	5	58	theme	human	745:749	arg1	subjects					800:807	32 subjects	797:807	32 subjects with medial OA	797:822	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	5	58	theme	human	745:749	arg1	subjects					784:791	10 healthy subjects	773:791	10 healthy subjects	773:791	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	5	58	theme	human	745:749	arg1	volunteers					751:760	Forty-two human volunteers	735:760	Forty-two human volunteers	735:760	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	10	59	theme	=	1620:1620	arg1	p					1618:1618	p = 0.310	1618:1626	p = 0.310	1618:1626	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	59	theme	=	1620:1620	arg1	controls					1592:1599	healthy controls	1584:1599	healthy controls	1584:1599	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	60	theme	gagCEST	1500:1506	arg1	asymmetry					1508:1516	average gagCEST asymmetry	1492:1516	average gagCEST asymmetry	1492:1516	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	7	61	theme	gagCEST	1098:1104	arg1	sequence					1106:1113	the volumetric gagCEST sequence	1083:1113	the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity	1083:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	1	62	theme	saturation	158:167	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	62	theme	saturation	158:167	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	13	63	theme	cartilage	2144:2152	arg1	composition					2154:2164	cartilage composition	2144:2164	cartilage composition in OA	2144:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	7	64	theme	Healthy	1001:1007	arg1	subjects					1009:1016	Healthy subjects	1001:1016	Healthy subjects	1001:1016	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	8	65	theme	p	1380:1380	arg1	0.015					1384:1388	p = 0.015	1380:1388	p = 0.015	1380:1388	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	8	65	theme	p	1380:1380	arg1	acquisitions					1366:1377	single-slice acquisitions	1353:1377	single-slice acquisitions (p = 0.015)	1353:1389	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	12	66	theme	high	1926:1929	arg1	asymmetry					1939:1947	high gagCEST asymmetry	1926:1947	high gagCEST asymmetry	1926:1947	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	11	67	theme	OA	1675:1676	arg1	subjects					1678:1685	OA subjects	1675:1685	OA subjects (p < 0.001 for both)	1675:1706	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	0	68	theme	osteoarthritic	113:126	arg1	population					128:137	an osteoarthritic population	110:137	an osteoarthritic population	110:137	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	10	69	theme	healthy	1544:1550	arg1	p					1562:1562	p = 0.655	1562:1570	p = 0.655	1562:1570	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	69	theme	healthy	1544:1550	arg1	controls					1552:1559	younger and older healthy controls	1526:1559	younger and older healthy controls (p = 0.655)	1526:1571	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	12	70	with	technique	1856:1864	arg1	times					1903:1907	volumetric scan times	1887:1907	volumetric scan times under 10 min and high gagCEST asymmetry at 3 T	1887:1954	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	3	71	theme	osteoarthritic	536:549	arg1	changes					551:557	osteoarthritic changes	536:557	osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures	536:628	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	9	72	theme	scan-rescan	1404:1414	arg1	%					1448:1448	6.8%	1445:1448	6.8%	1445:1448	The average scan-rescan coefficient of variation was 6.8%.					
32445515	9	72	theme	scan-rescan	1404:1414	arg1	coefficient					1416:1426	The average scan-rescan coefficient	1392:1426	The average scan-rescan coefficient of variation	1392:1439	The average scan-rescan coefficient of variation was 6.8%.					
32445515	6	73	theme	Osteoarthritis	869:882	arg1	Scores					892:897	Osteoarthritis Outcome Scores	869:897	Osteoarthritis Outcome Scores	869:897	Knee Injury and Osteoarthritis Outcome Scores (KOOS) were assessed for all subjects, and Kellgren-Lawrence grading was performed for OA volunteers.					
32445515	13	74	from	assessment	2113:2122	arg1	composition					2154:2164	cartilage composition	2144:2164	cartilage composition in OA	2144:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	7	75	theme	OA	1279:1280	arg1	severity					1282:1289	OA severity	1279:1289	OA severity	1279:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	10	76	theme	OA	1605:1606	arg1	subjects					1608:1615	OA subjects	1605:1615	OA subjects	1605:1615	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	3	77	theme	articular	567:575	arg1	cartilage					577:585	knee articular cartilage	562:585	knee articular cartilage	562:585	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	13	78	theme	limited	2066:2072	arg1	utility					2074:2080	a limited utility	2064:2080	a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA	2064:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	11	79	theme	T2	1630:1631	arg1	times					1652:1656	T2 and T1ρ relaxation times	1630:1656	times	1652:1656	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	1	80	theme	T	329:329	arg1	strengths					314:322	field strengths	308:322	field strengths of 7 T and higher	308:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	81	theme	Chemical	140:147	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	81	theme	Chemical	140:147	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	4	82	theme	gagCEST	651:657	arg1	asymmetry					659:667	gagCEST asymmetry	651:667	gagCEST asymmetry at 3 T	651:674	We hypothesize that gagCEST asymmetry at 3 T decreases with increasing severity of osteoarthritis (OA).					
32445515	3	83	from	T	503:503	arg1	sequence					459:466	a rapid volumetric gagCEST sequence	432:466	a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T	432:503	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	12	84	dep	times	1903:1907	arg1	scan					1898:1901	scan	1898:1901	scan	1898:1901	The gagCEST technique developed here, with volumetric scan times under 10 min and high gagCEST asymmetry at 3 T, did not vary significantly between healthy subjects and those with mild-moderate OA.					
32445515	4	85	theme	osteoarthritis	714:727	arg1	severity					702:709	severity	702:709	severity of osteoarthritis (OA)	702:732	We hypothesize that gagCEST asymmetry at 3 T decreases with increasing severity of osteoarthritis (OA).					
32445515	0	86	theme	dynamic	92:98	arg1	range					100:104	improved dynamic range	83:104	improved dynamic range	83:104	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	3	87	theme	work	410:413	arg1	objective					392:400	The objective	388:400	The objective of this work	388:413	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	0	88	theme	volumetric	6:15	arg1	imaging					25:31	Rapid volumetric gagCEST imaging	0:31	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	7	89	theme	femoral	1226:1232	arg1	cartilage					1244:1252	the femoral articular cartilage	1222:1252	the femoral articular cartilage	1222:1252	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	0	90	from	T	66:66	arg1	imaging					25:31	Rapid volumetric gagCEST imaging	0:31	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	7	91	theme	volumetric	1087:1096	arg1	sequence					1106:1113	the volumetric gagCEST sequence	1083:1113	the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity	1083:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	1	92	theme	cartilage	274:282	arg1	content					297:303	cartilage proteoglycan content	274:303	cartilage proteoglycan content	274:303	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	6	93	theme	OA	986:987	arg1	volunteers					989:998	OA volunteers	986:998	OA volunteers	986:998	Knee Injury and Osteoarthritis Outcome Scores (KOOS) were assessed for all subjects, and Kellgren-Lawrence grading was performed for OA volunteers.					
32445515	5	94	with	subjects	800:807	arg1	OA					821:822	medial OA	814:822	medial OA	814:822	Forty-two human volunteers, including 10 healthy subjects and 32 subjects with medial OA, were included in the study.					
32445515	8	95	theme	Volumetric	1292:1301	arg1	imaging					1311:1317	Volumetric gagCEST imaging	1292:1317	Volumetric gagCEST imaging	1292:1317	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	10	96	theme	healthy	1584:1590	arg1	p					1618:1618	p = 0.310	1618:1626	p = 0.310	1618:1626	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	10	96	theme	healthy	1584:1590	arg1	controls					1592:1599	healthy controls	1584:1599	healthy controls	1584:1599	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	0	97	theme	knee	36:39	arg1	cartilage					51:59	knee articular cartilage	36:59	knee articular cartilage	36:59	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	1	98	theme	MR	228:229	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	98	theme	MR	228:229	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	11	99	theme	KOOS	1786:1789	arg1	scores					1791:1796	total KOOS scores	1780:1796	total KOOS scores (rho = -0.181 and rho = -0.332 respectively)	1780:1841	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	0	100	theme	cartilage	51:59	arg1	imaging					25:31	Rapid volumetric gagCEST imaging	0:31	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	1	101	theme	field	308:312	arg1	strengths					314:322	field strengths	308:322	field strengths of 7 T and higher	308:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	7	102	theme	relaxation	1185:1194	arg1	times					1196:1200	gagCEST asymmetry and T2 and T1ρ relaxation times	1152:1200	times	1196:1200	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	8	103	contain	had	1319:1321	arg1	imaging					1311:1317	Volumetric gagCEST imaging	1292:1317	Volumetric gagCEST imaging	1292:1317	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	8	103	contain	had	1319:1321	arg2	asymmetry					1338:1346	higher gagCEST asymmetry	1323:1346	higher gagCEST asymmetry	1323:1346	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	7	104	dep	T.	1120:1121	arg1	calculated					1207:1216	calculated	1207:1216	were calculated for the femoral articular cartilage to assess sensitivity to OA severity	1202:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
32445515	8	105	theme	higher	1323:1328	arg1	asymmetry					1338:1346	higher gagCEST asymmetry	1323:1346	higher gagCEST asymmetry	1323:1346	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	10	106	from	differences	1477:1487	arg1	asymmetry					1508:1516	average gagCEST asymmetry	1492:1516	average gagCEST asymmetry	1492:1516	There were no significant differences in average gagCEST asymmetry between younger and older healthy controls (p = 0.655) or between healthy controls and OA subjects (p = 0.310).					
32445515	0	107	dep	imaging	25:31	arg1	evaluation					69:78	evaluation	69:78	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.	0:138	Rapid volumetric gagCEST imaging of knee articular cartilage at 3 T: evaluation of improved dynamic range and an osteoarthritic population.					
32445515	3	108	theme	volumetric	440:449	arg1	sequence					459:466	a rapid volumetric gagCEST sequence	432:466	a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T	432:503	The objective of this work was to implement a rapid volumetric gagCEST sequence with higher gagCEST asymmetry at 3 T to evaluate its sensitivity to osteoarthritic changes in knee articular cartilage and in comparison with T2 and T1ρ measures.					
32445515	1	109	theme	quantitative	215:226	arg1	technique					231:239	a quantitative MR technique	213:239	a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher	213:340	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	1	109	theme	quantitative	215:226	arg1	transfer					169:176	Chemical exchange saturation transfer	140:176	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST,	140:208	Chemical exchange saturation transfer of glycosaminoglycans, gagCEST, is a quantitative MR technique that has potential for assessing cartilage proteoglycan content at field strengths of 7 T and higher.					
32445515	13	110	theme	early	2127:2131	arg1	changes					2133:2139	early changes	2127:2139	early changes in cartilage composition in OA	2127:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	11	111	dep	scores	1791:1796	arg1	=					1803:1803	=	1803:1803	=	1803:1803	T2 and T1ρ relaxation times were elevated in OA subjects (p < 0.001 for both) compared with healthy controls and both were moderately correlated with total KOOS scores (rho = -0.181 and rho = -0.332 respectively).					
32445515	8	112	theme	single-slice	1353:1364	arg1	0.015					1384:1388	p = 0.015	1380:1388	p = 0.015	1380:1388	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	8	112	theme	single-slice	1353:1364	arg1	acquisitions					1366:1377	single-slice acquisitions	1353:1377	single-slice acquisitions (p = 0.015)	1353:1389	Volumetric gagCEST imaging had higher gagCEST asymmetry than single-slice acquisitions (p = 0.015).					
32445515	13	113	from	changes	2133:2139	arg1	composition					2154:2164	cartilage composition	2144:2164	cartilage composition in OA	2144:2170	This further supports a limited utility for gagCEST imaging at 3 T for assessment of early changes in cartilage composition in OA.					
32445515	7	114	theme	sequence	1106:1113	arg1	repeatability					1066:1078	the repeatability	1062:1078	the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity	1062:1289	Healthy subjects were scanned consecutively at 3 T to assess the repeatability of the volumetric gagCEST sequence at 3 T. For healthy and OA subjects, gagCEST asymmetry and T2 and T1ρ relaxation times were calculated for the femoral articular cartilage to assess sensitivity to OA severity.					
33245635	2	0	theme	ion	728:730	arg1	mode					743:746	selective ion monitoring mode	718:746	selective ion monitoring mode	718:746	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	9	1	from	hydrocarbons	1588:1599	arg1	matrix					1614:1619	any water matrix	1604:1619	any water matrix	1604:1619	These results indicate the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix.					
33245635	7	2	theme	single	1343:1348	arg1	extraction					1362:1371	a single solid-phase extraction	1341:1371	a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1341:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	2	3	theme	chromatography-mass	682:700	arg1	spectrometry					702:713	gas chromatography-mass spectrometry	678:713	gas chromatography-mass spectrometry	678:713	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	3	4	theme	aromatic	809:816	arg1	hydrocarbons					818:829	polycyclic aromatic hydrocarbons	798:829	polycyclic aromatic hydrocarbons	798:829	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	4	5	theme	prepared	976:983	arg1	aerogels					985:992	the prepared aerogels	972:992	the prepared aerogels	972:992	The results showed that the prepared aerogels have excellent adsorption capability for 16 target analytes.					
33245635	3	6	theme	pg/mL	854:858	arg1	level					860:864	the pg/mL level	850:864	the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk	850:945	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	9	7	theme	polycyclic	1568:1577	arg1	hydrocarbons					1588:1599	trace polycyclic aromatic hydrocarbons	1562:1599	trace polycyclic aromatic hydrocarbons in any water matrix	1562:1619	These results indicate the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix.					
33245635	7	8	from	cartridge	1373:1381	arg1	range					1404:1408	the concentration range	1386:1408	the concentration range of 10-2000 pg/mL	1386:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	1	9	theme	aromatic	209:216	arg1	hydrocarbons					218:229	Polycyclic aromatic hydrocarbons	198:229	Polycyclic aromatic hydrocarbons	198:229	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	1	9	theme	aromatic	209:216	arg1	compounds					243:251	ubiquitous compounds	232:251	ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources	232:346	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	4	10	theme	excellent	999:1007	arg1	capability					1020:1029	excellent adsorption capability	999:1029	excellent adsorption capability for 16 target analytes	999:1052	The results showed that the prepared aerogels have excellent adsorption capability for 16 target analytes.					
33245635	3	11	theme	aqueous	879:885	arg1	water					918:922	tap water	914:922	tap water	914:922	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	11	theme	aqueous	879:885	arg1	water					931:935	river water	925:935	river water	925:935	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	11	theme	aqueous	879:885	arg1	milk					942:945	milk	942:945	milk	942:945	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	11	theme	aqueous	879:885	arg1	matrices					894:901	different aqueous sample matrices	869:901	different aqueous sample matrices	869:901	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	2	12	theme	environmental	650:662	arg1	samples					664:670	environmental samples	650:670	environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode	650:746	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	7	13	from	range	1404:1408	arg1	cartridge					1373:1381	cartridge	1373:1381	cartridge	1373:1381	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	3	14	from	level	860:864	arg1	water					918:922	tap water	914:922	tap water	914:922	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	14	from	level	860:864	arg1	water					931:935	river water	925:935	river water	925:935	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	14	from	level	860:864	arg1	milk					942:945	milk	942:945	milk	942:945	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	14	from	level	860:864	arg1	matrices					894:901	different aqueous sample matrices	869:901	different aqueous sample matrices	869:901	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	0	15	theme	composite	133:141	arg1	aerogel					143:149	graphene/chitosan composite aerogel	115:149	graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry	115:195	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	5	16	dep	8.8	1098:1100	arg1	to					1095:1096	to	1095:1096	to	1095:1096	The limits of detection ranged from 1.7 to 8.8 pg/mL.					
33245635	0	17	theme	prior	151:155	arg1	aerogel					143:149	graphene/chitosan composite aerogel	115:149	graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry	115:195	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	4	18	theme	adsorption	1009:1018	arg1	capability					1020:1029	excellent adsorption capability	999:1029	excellent adsorption capability for 16 target analytes	999:1052	The results showed that the prepared aerogels have excellent adsorption capability for 16 target analytes.					
33245635	0	19	theme	gas	160:162	arg1	spectrometry					184:195	gas chromatography-mass spectrometry	160:195	gas chromatography-mass spectrometry	160:195	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	3	20	theme	river	925:929	arg1	water					931:935	river water	925:935	river water	925:935	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	6	21	theme	polycyclic	1127:1136	arg1	hydrocarbons					1147:1158	polycyclic aromatic hydrocarbons	1127:1158	polycyclic aromatic hydrocarbons spiked in river water	1127:1180	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	4	22	theme	target	1038:1043	arg1	analytes					1045:1052	16 target analytes	1035:1052	16 target analytes	1035:1052	The results showed that the prepared aerogels have excellent adsorption capability for 16 target analytes.					
33245635	9	23	theme	trace	1562:1566	arg1	hydrocarbons					1588:1599	trace polycyclic aromatic hydrocarbons	1562:1599	trace polycyclic aromatic hydrocarbons in any water matrix	1562:1619	These results indicate the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix.					
33245635	2	24	theme	hydrocarbon	577:587	arg1	extraction					589:598	efficient polycyclic aromatic hydrocarbon extraction	547:598	efficient polycyclic aromatic hydrocarbon extraction	547:598	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	8	25	theme	correlation	1458:1468	arg1	coefficients					1470:1481	excellent correlation coefficients	1448:1481	excellent correlation coefficients	1448:1481	The method provides excellent correlation coefficients over 0.9990.					
33245635	0	26	from	Determination	0:12	arg1	milk					69:72	milk	69:72	milk	69:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	0	26	from	Determination	0:12	arg1	water					59:63	water	59:63	water	59:63	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	7	27	theme	aromatic	1302:1309	arg1	hydrocarbon					1311:1321	polycyclic aromatic hydrocarbon	1291:1321	multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1282:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	1	28	theme	well-known	397:406	arg1	toxicity					408:415	their well-known toxicity	391:415	their well-known toxicity	391:415	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	2	29	theme	significant	446:456	arg1	potential					458:466	the significant potential	442:466	the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents	442:541	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	7	30	theme	multiple	1282:1289	arg1	extractions					1323:1333	multiple polycyclic aromatic hydrocarbon extractions	1282:1333	multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1282:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	2	31	theme	extraction	523:532	arg1	sorbents					534:541	solid-phase extraction sorbents	511:541	solid-phase extraction sorbents	511:541	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	1	32	theme	man-made	320:327	arg1	combustion					281:290	combustion	281:290	combustion	281:290	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	1	32	theme	man-made	320:327	arg1	pyrolysis					295:303	pyrolysis	295:303	pyrolysis	295:303	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	2	33	theme	composite	489:497	arg1	aerogels					499:506	graphene/chitosan composite aerogels	471:506	graphene/chitosan composite aerogels	471:506	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	0	34	theme	trace	17:21	arg1	hydrocarbons					43:54	trace polycyclic aromatic hydrocarbons	17:54	trace polycyclic aromatic hydrocarbons in water and milk	17:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	5	35	theme	detection	1069:1077	arg1	limits					1059:1064	The limits	1055:1064	The limits of detection	1055:1077	The limits of detection ranged from 1.7 to 8.8 pg/mL.					
33245635	2	36	theme	polycyclic	557:566	arg1	hydrocarbon					577:587	efficient polycyclic aromatic hydrocarbon	547:587	efficient polycyclic aromatic hydrocarbon extraction	547:598	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	6	37	dep	113	1202:1204	arg1	to					1199:1200	to	1199:1200	to	1199:1200	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	0	38	theme	aromatic	34:41	arg1	hydrocarbons					43:54	trace polycyclic aromatic hydrocarbons	17:54	trace polycyclic aromatic hydrocarbons in water and milk	17:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	7	39	theme	10-2000 pg/mL	1413:1425	arg1	range					1404:1408	the concentration range	1386:1408	the concentration range of 10-2000 pg/mL	1386:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	3	40	theme	optimal	759:765	arg1	condition					767:775	the optimal condition	755:775	the optimal condition	755:775	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	6	41	theme	standard	1222:1229	arg1	deviations					1231:1240	relative standard deviations	1213:1240	relative standard deviations of 1.1-6.6%	1213:1252	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	3	42	theme	polycyclic	798:807	arg1	hydrocarbons					818:829	polycyclic aromatic hydrocarbons	798:829	polycyclic aromatic hydrocarbons	798:829	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	6	43	theme	hydrocarbons	1147:1158	arg1	recoveries					1113:1122	The recoveries	1109:1122	The recoveries of polycyclic aromatic hydrocarbons spiked in river water	1109:1180	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	7	44	theme	cartridge	1373:1381	arg1	extraction					1362:1371	a single solid-phase extraction	1341:1371	a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1341:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	7	45	theme	hydrocarbon	1311:1321	arg1	extractions					1323:1333	multiple polycyclic aromatic hydrocarbon extractions	1282:1333	multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1282:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	2	46	theme	selective	718:726	arg1	mode					743:746	selective ion monitoring mode	718:746	selective ion monitoring mode	718:746	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	7	47	theme	solid-phase	1350:1360	arg1	extraction					1362:1371	a single solid-phase extraction	1341:1371	a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1341:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	1	48	theme	natural	308:314	arg1	combustion					281:290	combustion	281:290	combustion	281:290	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	1	48	theme	natural	308:314	arg1	pyrolysis					295:303	pyrolysis	295:303	pyrolysis	295:303	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	1	49	theme	Polycyclic	198:207	arg1	hydrocarbons					218:229	Polycyclic aromatic hydrocarbons	198:229	Polycyclic aromatic hydrocarbons	198:229	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	1	49	theme	Polycyclic	198:207	arg1	compounds					243:251	ubiquitous compounds	232:251	ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources	232:346	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	2	50	theme	monitoring	732:741	arg1	mode					743:746	selective ion monitoring mode	718:746	selective ion monitoring mode	718:746	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	6	51	theme	%	1252:1252	arg1	deviations					1231:1240	relative standard deviations	1213:1240	relative standard deviations of 1.1-6.6%	1213:1252	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	3	52	theme	sample	887:892	arg1	water					918:922	tap water	914:922	tap water	914:922	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	52	theme	sample	887:892	arg1	water					931:935	river water	925:935	river water	925:935	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	52	theme	sample	887:892	arg1	milk					942:945	milk	942:945	milk	942:945	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	52	theme	sample	887:892	arg1	matrices					894:901	different aqueous sample matrices	869:901	different aqueous sample matrices	869:901	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	9	53	theme	water	1608:1612	arg1	matrix					1614:1619	any water matrix	1604:1619	any water matrix	1604:1619	These results indicate the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix.					
33245635	3	54	theme	different	869:877	arg1	water					918:922	tap water	914:922	tap water	914:922	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	54	theme	different	869:877	arg1	water					931:935	river water	925:935	river water	925:935	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	54	theme	different	869:877	arg1	milk					942:945	milk	942:945	milk	942:945	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	3	54	theme	different	869:877	arg1	matrices					894:901	different aqueous sample matrices	869:901	different aqueous sample matrices	869:901	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	2	55	theme	sensitive	628:636	arg1	analysis					638:645	the highly sensitive analysis	617:645	the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode	617:746	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	0	56	from	milk	69:72	arg1	Determination					0:12	Determination	0:12	Determination of trace polycyclic aromatic hydrocarbons in water and milk	0:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	3	57	theme	tap	914:916	arg1	water					918:922	tap water	914:922	tap water	914:922	Under the optimal condition, samples spiked with polycyclic aromatic hydrocarbons were determined at the pg/mL level in different aqueous sample matrices, including tap water, river water, and milk.					
33245635	0	58	theme	chromatography-mass	164:182	arg1	spectrometry					184:195	gas chromatography-mass spectrometry	160:195	gas chromatography-mass spectrometry	160:195	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	9	59	theme	aromatic	1579:1586	arg1	hydrocarbons					1588:1599	trace polycyclic aromatic hydrocarbons	1562:1599	trace polycyclic aromatic hydrocarbons in any water matrix	1562:1619	These results indicate the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix.					
33245635	2	60	theme	gas	678:680	arg1	spectrometry					702:713	gas chromatography-mass spectrometry	678:713	gas chromatography-mass spectrometry	678:713	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	1	61	dep	natural	308:314	arg1	sources					340:346	combustion sources	329:346	combustion sources	329:346	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	6	62	theme	aromatic	1138:1145	arg1	hydrocarbons					1147:1158	polycyclic aromatic hydrocarbons	1127:1158	polycyclic aromatic hydrocarbons spiked in river water	1127:1180	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	1	63	theme	ubiquitous	232:241	arg1	hydrocarbons					218:229	Polycyclic aromatic hydrocarbons	198:229	Polycyclic aromatic hydrocarbons	198:229	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	1	63	theme	ubiquitous	232:241	arg1	compounds					243:251	ubiquitous compounds	232:251	ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources	232:346	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	0	64	theme	solid-phase	80:90	arg1	extraction					92:101	solid-phase extraction	80:101	solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry	80:195	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	4	65	contain	have	994:997	arg1	aerogels					985:992	the prepared aerogels	972:992	the prepared aerogels	972:992	The results showed that the prepared aerogels have excellent adsorption capability for 16 target analytes.					
33245635	4	65	contain	have	994:997	arg2	capability					1020:1029	excellent adsorption capability	999:1029	excellent adsorption capability for 16 target analytes	999:1052	The results showed that the prepared aerogels have excellent adsorption capability for 16 target analytes.					
33245635	2	66	theme	aromatic	568:575	arg1	hydrocarbon					577:587	efficient polycyclic aromatic hydrocarbon	547:587	efficient polycyclic aromatic hydrocarbon extraction	547:598	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	8	67	theme	excellent	1448:1456	arg1	coefficients					1470:1481	excellent correlation coefficients	1448:1481	excellent correlation coefficients	1448:1481	The method provides excellent correlation coefficients over 0.9990.					
33245635	7	68	theme	polycyclic	1291:1300	arg1	hydrocarbon					1311:1321	polycyclic aromatic hydrocarbon	1291:1321	multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL	1282:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	0	69	from	water	59:63	arg1	Determination					0:12	Determination	0:12	Determination of trace polycyclic aromatic hydrocarbons in water and milk	0:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	0	70	theme	graphene/chitosan	115:131	arg1	aerogel					143:149	graphene/chitosan composite aerogel	115:149	graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry	115:195	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	0	71	from	hydrocarbons	43:54	arg1	milk					69:72	milk	69:72	milk	69:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	0	71	from	hydrocarbons	43:54	arg1	water					59:63	water	59:63	water	59:63	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	2	72	theme	solid-phase	511:521	arg1	sorbents					534:541	solid-phase extraction sorbents	511:541	solid-phase extraction sorbents	511:541	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	0	73	theme	polycyclic	23:32	arg1	hydrocarbons					43:54	trace polycyclic aromatic hydrocarbons	17:54	trace polycyclic aromatic hydrocarbons in water and milk	17:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	1	74	theme	combustion	329:338	arg1	sources					340:346	combustion sources	329:346	combustion sources	329:346	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	6	75	theme	river	1170:1174	arg1	water					1176:1180	river water	1170:1180	river water	1170:1180	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	2	76	theme	aerogels	499:506	arg1	potential					458:466	the significant potential	442:466	the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents	442:541	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	2	77	theme	graphene/chitosan	471:487	arg1	aerogels					499:506	graphene/chitosan composite aerogels	471:506	graphene/chitosan composite aerogels	471:506	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	0	78	theme	hydrocarbons	43:54	arg1	Determination					0:12	Determination	0:12	Determination of trace polycyclic aromatic hydrocarbons in water and milk	0:72	Determination of trace polycyclic aromatic hydrocarbons in water and milk using solid-phase extraction packed with graphene/chitosan composite aerogel prior to gas chromatography-mass spectrometry.					
33245635	2	79	theme	efficient	547:555	arg1	hydrocarbon					577:587	efficient polycyclic aromatic hydrocarbon	547:587	efficient polycyclic aromatic hydrocarbon extraction	547:598	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	7	80	theme	concentration	1390:1402	arg1	range					1404:1408	the concentration range	1386:1408	the concentration range of 10-2000 pg/mL	1386:1425	The sorbent was reused for multiple polycyclic aromatic hydrocarbon extractions using a single solid-phase extraction cartridge in the concentration range of 10-2000 pg/mL.					
33245635	1	81	theme	human	371:375	arg1	health					377:382	human health	371:382	human health	371:382	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	6	82	theme	relative	1213:1220	arg1	deviations					1231:1240	relative standard deviations	1213:1240	relative standard deviations of 1.1-6.6%	1213:1252	The recoveries of polycyclic aromatic hydrocarbons spiked in river water ranged from 85.9 to 113%, with relative standard deviations of 1.1-6.6%.					
33245635	2	83	from	analysis	638:645	arg1	samples					664:670	environmental samples	650:670	environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode	650:746	This paper demonstrates the significant potential of graphene/chitosan composite aerogels as solid-phase extraction sorbents for efficient polycyclic aromatic hydrocarbon extraction and material for the highly sensitive analysis in environmental samples using gas chromatography-mass spectrometry in selective ion monitoring mode.					
33245635	1	84	theme	incomplete	270:279	arg1	combustion					281:290	combustion	281:290	combustion	281:290	Polycyclic aromatic hydrocarbons, ubiquitous compounds that result from incomplete combustion or pyrolysis of natural and man-made combustion sources, can adversely affects human health due to their well-known toxicity.					
33245635	9	85	theme	method	1542:1547	arg1	capability					1523:1532	the capability	1519:1532	the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix	1519:1619	These results indicate the capability of this method to determine trace polycyclic aromatic hydrocarbons in any water matrix.					
32515002	8	0	from	MFCs	1466:1469	arg1	performance					1429:1439	superior performance	1420:1439	superior performance of dye decolorization in MFCs	1420:1469	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	0	1	from	Decolorization	22:35	arg1	Cell					78:81	a Microbial Fuel Cell	61:81	a Microbial Fuel Cell	61:81	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	7	2	theme	polyaniline	1118:1128	arg1	modification					1102:1113	the modification	1098:1113	the modification of polyaniline and chitosan-NCNTs	1098:1147	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	1	3	theme	recent	146:151	arg1	years					153:157	recent years	146:157	recent years	146:157	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	1	4	theme	promising	209:217	arg1	technology					219:228	a promising technology	207:228	a promising technology for dye wastewater treatment	207:257	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	1	4	theme	promising	209:217	arg1	cell					175:178	microbial fuel cell	160:178	microbial fuel cell (MFC)	160:184	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	2	5	contain	has	310:312	arg2	effect					336:341	better decolorization effect	314:341	better decolorization effect	314:341	Compared with traditional anaerobic reactors, MFC has better decolorization effect while producing electricity simultaneously.					
32515002	2	5	contain	has	310:312	arg1	MFC					306:308	MFC	306:308	MFC	306:308	Compared with traditional anaerobic reactors, MFC has better decolorization effect while producing electricity simultaneously.					
32515002	8	6	theme	synergistic	1370:1380	arg1	role					1382:1385	the synergistic role	1366:1385	the synergistic role of various materials	1366:1406	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	2	7	theme	decolorization	321:334	arg1	effect					336:341	better decolorization effect	314:341	better decolorization effect	314:341	Compared with traditional anaerobic reactors, MFC has better decolorization effect while producing electricity simultaneously.					
32515002	6	8	theme	modified	987:994	arg1	surface					1004:1010	the modified anode's surface	983:1010	the modified anode's surface	983:1010	And the biomass on the modified anode's surface was increased by 1.47 times.					
32515002	0	9	theme	Sponge	92:97	arg1	Anode					99:103	the Sponge Anode	88:103	the Sponge Anode	88:103	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	9	10	theme	decolorization	1557:1570	arg1	recovery					1526:1533	the recovery	1522:1533	the recovery of biomass energy and decolorization in wastewater treatment	1522:1594	This work provided a strategy for the research on the recovery of biomass energy and decolorization in wastewater treatment.					
32515002	5	11	dep	biocompatibility	901:916	arg1	the					897:899	the	897:899	the	897:899	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	8	12	theme	various	1390:1396	arg1	materials					1398:1406	various materials	1390:1406	various materials	1390:1406	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	3	13	theme	bioelectricity	545:558	arg1	generation					560:569	bioelectricity generation	545:569	bioelectricity generation	545:569	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	8	14	from	performance	1429:1439	arg1	MFCs					1466:1469	MFCs	1466:1469	MFCs	1466:1469	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	1	15	theme	dye	234:236	arg1	treatment					249:257	dye wastewater treatment	234:257	dye wastewater treatment	234:257	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	4	16	theme	operating	640:648	arg1	temperature					650:660	operating temperature	640:660	operating temperature	640:660	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	3	17	theme	dye	522:524	arg1	decolorization					526:539	simultaneous azo dye decolorization	505:539	simultaneous azo dye decolorization	505:539	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	3	18	theme	double-chamber	404:417	arg1	MFC					419:421	a double-chamber MFC	402:421	a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs	402:486	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	5	19	theme	MFC	839:841	arg1	efficiency					773:782	a high decolorization efficiency	751:782	a high decolorization efficiency (98.41%)	751:791	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	19	theme	MFC	839:841	arg1	%					790:790	98.41%	785:790	98.41%	785:790	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	19	theme	MFC	839:841	arg1	m-3					831:833	2816.67 mW m-3	820:833	2816.67 mW m-3	820:833	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	19	theme	MFC	839:841	arg1	density					811:817	maximum power density	797:817	maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes	797:874	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	4	20	theme	concentration	593:605	arg1	influence					576:584	The influence	572:584	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes	572:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	0	21	from	Generation	47:56	arg1	Cell					78:81	a Microbial Fuel Cell	61:81	a Microbial Fuel Cell	61:81	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	4	22	theme	dye	589:591	arg1	concentration					593:605	dye concentration	589:605	dye concentration	589:605	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	9	23	from	research	1510:1517	arg1	recovery					1526:1533	the recovery	1522:1533	the recovery of biomass energy and decolorization in wastewater treatment	1522:1594	This work provided a strategy for the research on the recovery of biomass energy and decolorization in wastewater treatment.					
32515002	7	24	theme	communities	1195:1205	arg1	enrichment					1172:1181	the selective enrichment	1158:1181	the selective enrichment of specific communities	1158:1205	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	1	25	theme	microbial	160:168	arg1	technology					219:228	a promising technology	207:228	a promising technology for dye wastewater treatment	207:257	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	1	25	theme	microbial	160:168	arg1	cell					175:178	microbial fuel cell	160:178	microbial fuel cell (MFC)	160:184	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	1	25	theme	microbial	160:168	arg1	MFC					181:183	MFC	181:183	MFC	181:183	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	0	26	theme	Simultaneous	9:20	arg1	Decolorization					22:35	Improved Simultaneous Decolorization	0:35	Improved Simultaneous Decolorization	0:35	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	5	27	theme	decolorization	758:771	arg1	efficiency					773:782	a high decolorization efficiency	751:782	a high decolorization efficiency (98.41%)	751:791	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	27	theme	decolorization	758:771	arg1	%					790:790	98.41%	785:790	98.41%	785:790	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	9	28	theme	wastewater	1575:1584	arg1	treatment					1586:1594	wastewater treatment	1575:1594	wastewater treatment	1575:1594	This work provided a strategy for the research on the recovery of biomass energy and decolorization in wastewater treatment.					
32515002	7	29	theme	specific	1186:1193	arg1	communities					1195:1205	specific communities	1186:1205	specific communities	1186:1205	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	1	30	theme	fuel	170:173	arg1	technology					219:228	a promising technology	207:228	a promising technology for dye wastewater treatment	207:257	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	1	30	theme	fuel	170:173	arg1	cell					175:178	microbial fuel cell	160:178	microbial fuel cell (MFC)	160:184	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	1	30	theme	fuel	170:173	arg1	MFC					181:183	MFC	181:183	MFC	181:183	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	0	31	theme	Improved	0:7	arg1	Decolorization					22:35	Improved Simultaneous Decolorization	0:35	Improved Simultaneous Decolorization	0:35	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	1	32	theme	wastewater	238:247	arg1	treatment					249:257	dye wastewater treatment	234:257	dye wastewater treatment	234:257	In recent years, microbial fuel cell (MFC) has been regarded as a promising technology for dye wastewater treatment.					
32515002	2	33	theme	anaerobic	286:294	arg1	reactors					296:303	traditional anaerobic reactors	274:303	traditional anaerobic reactors	274:303	Compared with traditional anaerobic reactors, MFC has better decolorization effect while producing electricity simultaneously.					
32515002	5	34	theme	power	805:809	arg1	density					811:817	maximum power density	797:817	maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes	797:874	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	34	theme	power	805:809	arg1	m-3					831:833	2816.67 mW m-3	820:833	2816.67 mW m-3	820:833	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	4	35	theme	MFC	684:686	arg1	performance					669:679	the performance	665:679	the performance of MFC with the modified anodes	665:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	7	36	theme	microbial	1055:1063	arg1	analysis					1075:1082	microbial community analysis	1055:1082	microbial community analysis	1055:1082	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	9	37	from	recovery	1526:1533	arg1	treatment					1586:1594	wastewater treatment	1575:1594	wastewater treatment	1575:1594	This work provided a strategy for the research on the recovery of biomass energy and decolorization in wastewater treatment.					
32515002	2	38	theme	traditional	274:284	arg1	reactors					296:303	traditional anaerobic reactors	274:303	traditional anaerobic reactors	274:303	Compared with traditional anaerobic reactors, MFC has better decolorization effect while producing electricity simultaneously.					
32515002	4	39	theme	temperature	650:660	arg1	influence					576:584	The influence	572:584	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes	572:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	7	40	theme	community	1065:1073	arg1	analysis					1075:1082	microbial community analysis	1055:1082	microbial community analysis	1055:1082	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	5	41	theme	material	954:961	arg1	bioelectrocatalysis					922:940	bioelectrocatalysis	922:940	bioelectrocatalysis	922:940	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	41	theme	material	954:961	arg1	biocompatibility					901:916	biocompatibility	901:916	biocompatibility	901:916	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	9	42	theme	biomass	1538:1544	arg1	energy					1546:1551	biomass energy	1538:1551	biomass energy	1538:1551	This work provided a strategy for the research on the recovery of biomass energy and decolorization in wastewater treatment.					
32515002	4	43	theme	modified	697:704	arg1	anodes					706:711	the modified anodes	693:711	the modified anodes	693:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	6	44	from	biomass	972:978	arg1	surface					1004:1010	the modified anode's surface	983:1010	the modified anode's surface	983:1010	And the biomass on the modified anode's surface was increased by 1.47 times.					
32515002	5	45	theme	modified	857:864	arg1	bioanodes					866:874	modified bioanodes	857:874	modified bioanodes	857:874	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	3	46	theme	sponge	432:437	arg1	anode					439:443	the sponge anode	428:443	the sponge anode modified by polyaniline and chitosan-NCNTs	428:486	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	0	47	theme	Power	41:45	arg1	Generation					47:56	Power Generation	41:56	Power Generation	41:56	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	7	48	theme	selective	1162:1170	arg1	enrichment					1172:1181	the selective enrichment	1158:1181	the selective enrichment of specific communities	1158:1205	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	8	49	from	decolorization	1448:1461	arg1	MFCs					1466:1469	MFCs	1466:1469	MFCs	1466:1469	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	5	50	theme	high	753:756	arg1	efficiency					773:782	a high decolorization efficiency	751:782	a high decolorization efficiency (98.41%)	751:791	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	50	theme	high	753:756	arg1	%					790:790	98.41%	785:790	98.41%	785:790	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	7	51	theme	chitosan-NCNTs	1134:1147	arg1	modification					1102:1113	the modification	1098:1113	the modification of polyaniline and chitosan-NCNTs	1098:1147	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	52	theme	main	1215:1218	arg1	microorganism					1220:1232	the main microorganism	1211:1232	the main microorganism	1211:1232	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	52	theme	main	1215:1218	arg1	bacteria					1273:1280	the electroactive and decolorizing bacteria	1238:1280	the electroactive and decolorizing bacteria Enterobacter (62.84%)	1238:1302	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	4	53	with	performance	669:679	arg1	anodes					706:711	the modified anodes	693:711	the modified anodes	693:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	8	54	theme	superior	1420:1427	arg1	performance					1429:1439	superior performance	1420:1439	superior performance of dye decolorization in MFCs	1420:1469	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	0	55	with	Decolorization	22:35	arg1	Anode					99:103	the Sponge Anode	88:103	the Sponge Anode	88:103	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	2	56	theme	better	314:319	arg1	effect					336:341	better decolorization effect	314:341	better decolorization effect	314:341	Compared with traditional anaerobic reactors, MFC has better decolorization effect while producing electricity simultaneously.					
32515002	5	57	theme	maximum	797:803	arg1	density					811:817	maximum power density	797:817	maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes	797:874	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	57	theme	maximum	797:803	arg1	m-3					831:833	2816.67 mW m-3	820:833	2816.67 mW m-3	820:833	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	3	58	with	MFC	419:421	arg1	anode					439:443	the sponge anode	428:443	the sponge anode modified by polyaniline and chitosan-NCNTs	428:486	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	10	59	dep	Abstract	1607:1614	arg1	Graphical					1597:1605	Graphical	1597:1605	Graphical	1597:1605	Graphical Abstract.					
32515002	7	60	theme	electroactive	1242:1254	arg1	Enterobacter					1282:1293	Enterobacter	1282:1293	Enterobacter	1282:1293	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	60	theme	electroactive	1242:1254	arg1	%					1301:1301	62.84%	1296:1301	62.84%	1296:1301	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	60	theme	electroactive	1242:1254	arg1	microorganism					1220:1232	the main microorganism	1211:1232	the main microorganism	1211:1232	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	60	theme	electroactive	1242:1254	arg1	bacteria					1273:1280	the electroactive and decolorizing bacteria	1238:1280	the electroactive and decolorizing bacteria Enterobacter (62.84%)	1238:1302	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	4	61	from	influence	576:584	arg1	performance					669:679	the performance	665:679	the performance of MFC with the modified anodes	665:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	8	62	theme	composite	1320:1328	arg1	capable					1339:1345	capable	1339:1345	capable	1339:1345	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	8	62	theme	composite	1320:1328	arg1	anode					1330:1334	the composite anode	1316:1334	the composite anode	1316:1334	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	5	63	theme	modified	945:952	arg1	material					954:961	modified material	945:961	modified material	945:961	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	4	64	theme	concentration	621:633	arg1	influence					576:584	The influence	572:584	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes	572:711	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	9	65	theme	energy	1546:1551	arg1	recovery					1526:1533	the recovery	1522:1533	the recovery of biomass energy and decolorization in wastewater treatment	1522:1594	This work provided a strategy for the research on the recovery of biomass energy and decolorization in wastewater treatment.					
32515002	4	66	theme	co-substrate	608:619	arg1	concentration					621:633	co-substrate concentration	608:633	co-substrate concentration	608:633	The influence of dye concentration, co-substrate concentration, and operating temperature on the performance of MFC with the modified anodes were studied.					
32515002	0	67	theme	Fuel	73:76	arg1	Cell					78:81	a Microbial Fuel Cell	61:81	a Microbial Fuel Cell	61:81	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	8	68	theme	materials	1398:1406	arg1	role					1382:1385	the synergistic role	1366:1385	the synergistic role of various materials	1366:1406	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	8	69	theme	decolorization	1448:1461	arg1	performance					1429:1439	superior performance	1420:1439	superior performance of dye decolorization in MFCs	1420:1469	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	5	70	theme	mW	828:829	arg1	density					811:817	maximum power density	797:817	maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes	797:874	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	5	70	theme	mW	828:829	arg1	m-3					831:833	2816.67 mW m-3	820:833	2816.67 mW m-3	820:833	The results showed that a high decolorization efficiency (98.41%) and maximum power density (2816.67 mW m-3) of MFC equipped with modified bioanodes were achieved due to the biocompatibility and bioelectrocatalysis of modified material.					
32515002	3	71	theme	simultaneous	505:516	arg1	decolorization					526:539	simultaneous azo dye decolorization	505:539	simultaneous azo dye decolorization	505:539	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	0	72	theme	Microbial	63:71	arg1	Cell					78:81	a Microbial Fuel Cell	61:81	a Microbial Fuel Cell	61:81	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	8	73	theme	dye	1444:1446	arg1	decolorization					1448:1461	dye decolorization	1444:1461	dye decolorization in MFCs	1444:1469	Therefore, the composite anode is capable of fully utilizing the synergistic role of various materials, leading to superior performance of dye decolorization in MFCs.					
32515002	0	74	with	Generation	47:56	arg1	Anode					99:103	the Sponge Anode	88:103	the Sponge Anode	88:103	Improved Simultaneous Decolorization and Power Generation in a Microbial Fuel Cell with the Sponge Anode Modified by Polyaniline and Chitosan.					
32515002	3	75	theme	azo	518:520	arg1	decolorization					526:539	simultaneous azo dye decolorization	505:539	simultaneous azo dye decolorization	505:539	In this paper, a double-chamber MFC with the sponge anode modified by polyaniline and chitosan-NCNTs was employed for simultaneous azo dye decolorization and bioelectricity generation.					
32515002	7	76	theme	decolorizing	1260:1271	arg1	Enterobacter					1282:1293	Enterobacter	1282:1293	Enterobacter	1282:1293	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	76	theme	decolorizing	1260:1271	arg1	%					1301:1301	62.84%	1296:1301	62.84%	1296:1301	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	76	theme	decolorizing	1260:1271	arg1	microorganism					1220:1232	the main microorganism	1211:1232	the main microorganism	1211:1232	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32515002	7	76	theme	decolorizing	1260:1271	arg1	bacteria					1273:1280	the electroactive and decolorizing bacteria	1238:1280	the electroactive and decolorizing bacteria Enterobacter (62.84%)	1238:1302	Additionally, microbial community analysis revealed that the modification of polyaniline and chitosan-NCNTs improved the selective enrichment of specific communities and the main microorganism was the electroactive and decolorizing bacteria Enterobacter (62.84%).					
32634511	2	0	theme	blue	502:505	arg1	dye					507:509	methylene blue dye	492:509	methylene blue dye	492:509	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	4	1	theme	dye	885:887	arg1	30-400 mg/L					904:914	30-400 mg/L	904:914	30-400 mg/L	904:914	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	1	theme	dye	885:887	arg1	concentration					889:901	initial dye concentration	877:901	initial dye concentration (30-400 mg/L)	877:915	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	1	theme	dye	885:887	arg1	dosage					812:817	CHS-ECH/ZL dosage	801:817	CHS-ECH/ZL dosage (0.02-0.5 g)	801:830	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	2	2	theme	aqueous	569:575	arg1	solutions					577:585	aqueous solutions	569:585	aqueous solutions	569:585	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	4	3	from	influence	760:768	arg1	removal					967:973	the MB and RR120 removal	950:973	removal	967:973	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	0	4	theme	red	100:102	arg1	chitosan-epichlorohydrin/zeolite					21:52	Zwitterion composite chitosan-epichlorohydrin/zeolite	0:52	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.	0:112	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.					
32634511	5	5	theme	recorded	1132:1139	arg1	156.1					1181:1185	156.1	1181:1185	156.1	1181:1185	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	5	5	theme	recorded	1132:1139	arg1	capacities					1152:1161	the recorded adsorption capacities	1128:1161	the recorded adsorption capacities of CHS-ECH/ZL	1128:1175	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	4	6	theme	CHS-ECH/ZL	801:810	arg1	0.02-0.5 g					820:829	0.02-0.5 g	820:829	0.02-0.5 g	820:829	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	6	theme	CHS-ECH/ZL	801:810	arg1	temperature					853:863	temperature	853:863	temperature (303-323K)	853:874	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	6	theme	CHS-ECH/ZL	801:810	arg1	time					930:933	contact time	922:933	contact time	922:933	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	6	theme	CHS-ECH/ZL	801:810	arg1	concentration					889:901	initial dye concentration	877:901	initial dye concentration (30-400 mg/L)	877:915	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	6	theme	CHS-ECH/ZL	801:810	arg1	dosage					812:817	CHS-ECH/ZL dosage	801:817	CHS-ECH/ZL dosage (0.02-0.5 g)	801:830	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	6	theme	CHS-ECH/ZL	801:810	arg1	pH					842:843	solution pH	833:843	solution pH (4-10)	833:850	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	6	7	theme	adsorption	1272:1281	arg1	mechanism					1246:1254	The mechanism	1242:1254	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL	1242:1301	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	4	8	theme	solution	833:840	arg1	dosage					812:817	CHS-ECH/ZL dosage	801:817	CHS-ECH/ZL dosage (0.02-0.5 g)	801:830	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	8	theme	solution	833:840	arg1	4-10					846:849	4-10	846:849	4-10	846:849	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	8	theme	solution	833:840	arg1	0-600 min					936:944	0-600 min	936:944	0-600 min	936:944	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	8	theme	solution	833:840	arg1	pH					842:843	solution pH	833:843	solution pH (4-10)	833:850	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	5	9	theme	adsorption	1141:1150	arg1	156.1					1181:1185	156.1	1181:1185	156.1	1181:1185	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	5	9	theme	adsorption	1141:1150	arg1	capacities					1152:1161	the recorded adsorption capacities	1128:1161	the recorded adsorption capacities of CHS-ECH/ZL	1128:1175	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	4	10	theme	initial	877:883	arg1	30-400 mg/L					904:914	30-400 mg/L	904:914	30-400 mg/L	904:914	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	10	theme	initial	877:883	arg1	concentration					889:901	initial dye concentration	877:901	initial dye concentration (30-400 mg/L)	877:915	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	10	theme	initial	877:883	arg1	dosage					812:817	CHS-ECH/ZL dosage	801:817	CHS-ECH/ZL dosage (0.02-0.5 g)	801:830	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	2	11	dep	anionic	521:527	arg1	RR120					552:556	reactive red 120 dye, RR120	530:556	RR120	552:556	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	2	12	dep	cationic	482:489	arg1	dye					507:509	methylene blue dye	492:509	methylene blue dye	492:509	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	7	13	theme	aqueous	1639:1645	arg1	environment					1647:1657	aqueous environment	1639:1657	aqueous environment	1639:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	7	14	theme	different	1598:1606	arg1	dyes					1629:1632	the two structurally different cationic and anionic dyes	1577:1632	the two structurally different cationic and anionic dyes from aqueous environment	1577:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	1	15	dep	zeolite	310:316	arg1	ZL					319:320	ZL	319:320	ZL	319:320	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
32634511	7	16	theme	eco-friendly	1511:1522	arg1	zwitterion					1524:1533	an eco-friendly zwitterion	1508:1533	an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment	1508:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	7	16	theme	eco-friendly	1511:1522	arg1	composite					1495:1503	CHS-ECH/ZL composite	1484:1503	CHS-ECH/ZL composite	1484:1503	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	5	17	theme	CHS-ECH/ZL	1166:1175	arg1	156.1					1181:1185	156.1	1181:1185	156.1	1181:1185	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	5	17	theme	CHS-ECH/ZL	1166:1175	arg1	capacities					1152:1161	the recorded adsorption capacities	1128:1161	the recorded adsorption capacities of CHS-ECH/ZL	1128:1175	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	1	18	theme	adsorbent	175:183	arg1	zwitterion					154:163	zwitterion	154:163	zwitterion composite adsorbent	154:183	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
32634511	1	19	dep	aluminosilicate	285:299	arg1	zeolite					310:316	zeolite	310:316	zeolite	310:316	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
32634511	6	20	theme	various	1313:1319	arg1	types					1321:1325	various types	1313:1325	various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction	1313:1447	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	7	21	theme	CHS-ECH/ZL	1484:1493	arg1	zwitterion					1524:1533	an eco-friendly zwitterion	1508:1533	an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment	1508:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	7	21	theme	CHS-ECH/ZL	1484:1493	arg1	composite					1495:1503	CHS-ECH/ZL composite	1484:1503	CHS-ECH/ZL composite	1484:1503	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	0	22	theme	composite	11:19	arg1	chitosan-epichlorohydrin/zeolite					21:52	Zwitterion composite chitosan-epichlorohydrin/zeolite	0:52	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.	0:112	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.					
32634511	2	23	theme	methylene	492:500	arg1	dye					507:509	methylene blue dye	492:509	methylene blue dye	492:509	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	4	24	theme	parameters	783:792	arg1	influence					760:768	The influence	756:768	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal	756:973	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	2	25	theme	chitosan-epichlorohydrin/zeolite	362:393	arg1	zwitterion					328:337	The zwitterion composite adsorbent	324:357	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL)	324:406	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	0	26	theme	Zwitterion	0:9	arg1	chitosan-epichlorohydrin/zeolite					21:52	Zwitterion composite chitosan-epichlorohydrin/zeolite	0:52	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.	0:112	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.					
32634511	7	27	theme	adsorbent	1535:1543	arg1	zwitterion					1524:1533	an eco-friendly zwitterion	1508:1533	an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment	1508:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	7	27	theme	adsorbent	1535:1543	arg1	composite					1495:1503	CHS-ECH/ZL composite	1484:1503	CHS-ECH/ZL composite	1484:1503	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	4	28	theme	pertinent	773:781	arg1	parameters					783:792	pertinent parameters	773:792	pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min)	773:945	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	7	29	with	adsorbent	1535:1543	arg1	applicability					1555:1567	good applicability	1550:1567	good applicability towards the two structurally different cationic and anionic dyes from aqueous environment	1550:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	7	30	from	environment	1647:1657	arg1	dyes					1629:1632	the two structurally different cationic and anionic dyes	1577:1632	the two structurally different cationic and anionic dyes from aqueous environment	1577:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	5	31	from	30 °C	1235:1239	arg1	284.2 mg/g					1191:1200	284.2 mg/g	1191:1200	284.2 mg/g	1191:1200	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	5	31	from	30 °C	1235:1239	arg1	156.1					1181:1185	156.1	1181:1185	156.1	1181:1185	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	5	31	from	30 °C	1235:1239	arg1	capacities					1152:1161	the recorded adsorption capacities	1128:1161	the recorded adsorption capacities of CHS-ECH/ZL	1128:1175	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	2	32	theme	red	539:541	arg1	RR120					552:556	reactive red 120 dye, RR120	530:556	RR120	552:556	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	2	33	theme	multifunctional	421:435	arg1	tasks					437:441	multifunctional tasks	421:441	multifunctional tasks	421:441	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	4	34	theme	contact	922:928	arg1	dosage					812:817	CHS-ECH/ZL dosage	801:817	CHS-ECH/ZL dosage (0.02-0.5 g)	801:830	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	34	theme	contact	922:928	arg1	time					930:933	contact time	922:933	contact time	922:933	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	5	35	theme	research	991:998	arg1	findings					1000:1007	The research findings	987:1007	The research findings	987:1007	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	2	36	theme	reactive	530:537	arg1	RR120					552:556	reactive red 120 dye, RR120	530:556	RR120	552:556	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	7	37	theme	good	1550:1553	arg1	applicability					1555:1567	good applicability	1550:1567	good applicability towards the two structurally different cationic and anionic dyes from aqueous environment	1550:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	7	38	theme	cationic	1608:1615	arg1	dyes					1629:1632	the two structurally different cationic and anionic dyes	1577:1632	the two structurally different cationic and anionic dyes from aqueous environment	1577:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	6	39	theme	interactions	1330:1341	arg1	types					1321:1325	various types	1313:1325	various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction	1313:1447	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	0	40	dep	blue	82:85	arg1	dyes					108:111	120 dyes	104:111	120 dyes	104:111	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.					
32634511	2	41	theme	cationic	482:489	arg1	dyes					559:562	cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes	482:562	two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes	455:562	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	2	42	theme	anionic	521:527	arg1	dyes					559:562	cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes	482:562	two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes	455:562	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	2	43	theme	different	472:480	arg1	dyes					559:562	cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes	482:562	two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes	455:562	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	1	44	theme	mineral	301:307	arg1	aluminosilicate					285:299	an aluminosilicate mineral	282:307	an aluminosilicate mineral (zeolite, ZL)	282:321	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
32634511	6	45	theme	hydrogen	1378:1385	arg1	bonding					1387:1393	hydrogen bonding	1378:1393	hydrogen bonding	1378:1393	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	6	45	theme	hydrogen	1378:1385	arg1	interactions					1330:1341	interactions	1330:1341	interactions namely	1330:1348	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	6	46	theme	MB	1259:1260	arg1	mechanism					1246:1254	The mechanism	1242:1254	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL	1242:1301	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	3	47	theme	CHS-ECH/ZL	670:679	arg1	crystallinity					610:622	crystallinity	610:622	crystallinity	610:622	The surface property, crystallinity, morphology, functionality, and charge of the CHS-ECH/ZL are analyzed using BET, XRD, SEM, FTIR, and pHpzc, analyses, respectively.					
32634511	3	47	theme	CHS-ECH/ZL	670:679	arg1	property					600:607	The surface property	588:607	The surface property	588:607	The surface property, crystallinity, morphology, functionality, and charge of the CHS-ECH/ZL are analyzed using BET, XRD, SEM, FTIR, and pHpzc, analyses, respectively.					
32634511	3	47	theme	CHS-ECH/ZL	670:679	arg1	functionality					637:649	functionality	637:649	functionality	637:649	The surface property, crystallinity, morphology, functionality, and charge of the CHS-ECH/ZL are analyzed using BET, XRD, SEM, FTIR, and pHpzc, analyses, respectively.					
32634511	3	47	theme	CHS-ECH/ZL	670:679	arg1	charge					656:661	charge	656:661	charge	656:661	The surface property, crystallinity, morphology, functionality, and charge of the CHS-ECH/ZL are analyzed using BET, XRD, SEM, FTIR, and pHpzc, analyses, respectively.					
32634511	3	47	theme	CHS-ECH/ZL	670:679	arg1	morphology					625:634	morphology	625:634	morphology	625:634	The surface property, crystallinity, morphology, functionality, and charge of the CHS-ECH/ZL are analyzed using BET, XRD, SEM, FTIR, and pHpzc, analyses, respectively.					
32634511	6	48	theme	RR120	1266:1270	arg1	adsorption					1272:1281	RR120 adsorption	1266:1281	RR120 adsorption onto the CHS-ECH/ZL	1266:1301	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	5	49	theme	adsorption	1027:1036	arg1	isotherm					1038:1045	the adsorption isotherm	1023:1045	the adsorption isotherm at equilibrium	1023:1060	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	4	50	dep	parameters	783:792	arg1	0.02-0.5 g					820:829	0.02-0.5 g	820:829	0.02-0.5 g	820:829	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	50	dep	parameters	783:792	arg1	temperature					853:863	temperature	853:863	temperature (303-323K)	853:874	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	50	dep	parameters	783:792	arg1	time					930:933	contact time	922:933	contact time	922:933	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	50	dep	parameters	783:792	arg1	concentration					889:901	initial dye concentration	877:901	initial dye concentration (30-400 mg/L)	877:915	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	50	dep	parameters	783:792	arg1	dosage					812:817	CHS-ECH/ZL dosage	801:817	CHS-ECH/ZL dosage (0.02-0.5 g)	801:830	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	50	dep	parameters	783:792	arg1	pH					842:843	solution pH	833:843	solution pH (4-10)	833:850	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	4	51	theme	MB	954:955	arg1	removal					967:973	the MB and RR120 removal	950:973	removal	967:973	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	2	52	theme	dye	547:549	arg1	RR120					552:556	reactive red 120 dye, RR120	530:556	RR120	552:556	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	6	53	theme	n-π	1433:1435	arg1	interaction					1437:1447	n-π interaction	1433:1447	n-π interaction	1433:1447	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	5	54	theme	Freundlich	1097:1106	arg1	model					1117:1121	the Freundlich isotherm model	1093:1121	the Freundlich isotherm model	1093:1121	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	2	55	dep	dye	507:509	arg1	MB					512:513	MB	512:513	MB	512:513	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	1	56	dep	cross-linker	242:253	arg1	ECH					273:275	ECH	273:275	ECH	273:275	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
32634511	1	56	dep	cross-linker	242:253	arg1	epichlorohydrin					256:270	epichlorohydrin	256:270	epichlorohydrin	256:270	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
32634511	3	57	theme	surface	592:598	arg1	property					600:607	The surface property	588:607	The surface property	588:607	The surface property, crystallinity, morphology, functionality, and charge of the CHS-ECH/ZL are analyzed using BET, XRD, SEM, FTIR, and pHpzc, analyses, respectively.					
32634511	4	58	theme	RR120	961:965	arg1	removal					967:973	the MB and RR120 removal	950:973	removal	967:973	The influence of pertinent parameters namely CHS-ECH/ZL dosage (0.02-0.5 g), solution pH (4-10), temperature (303-323K), initial dye concentration (30-400 mg/L), and contact time (0-600 min) on the MB and RR120 removal are tested.					
32634511	5	59	theme	isotherm	1108:1115	arg1	model					1117:1121	the Freundlich isotherm model	1093:1121	the Freundlich isotherm model	1093:1121	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	5	60	from	equilibrium	1050:1060	arg1	isotherm					1038:1045	the adsorption isotherm	1023:1045	the adsorption isotherm at equilibrium	1023:1060	The research findings revealed that the adsorption isotherm at equilibrium well explained in according to the Freundlich isotherm model, and the recorded adsorption capacities of CHS-ECH/ZL are 156.1 and 284.2 mg/g for MB and RR120 respectively at 30 °C.					
32634511	7	61	theme	anionic	1621:1627	arg1	dyes					1629:1632	the two structurally different cationic and anionic dyes	1577:1632	the two structurally different cationic and anionic dyes from aqueous environment	1577:1657	Overall, this research introduces CHS-ECH/ZL composite as an eco-friendly zwitterion adsorbent with good applicability towards the two structurally different cationic and anionic dyes from aqueous environment.					
32634511	2	62	theme	adsorbent	349:357	arg1	zwitterion					328:337	The zwitterion composite adsorbent	324:357	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL)	324:406	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	6	63	theme	electrostatic	1351:1363	arg1	interaction					1365:1375	electrostatic interaction	1351:1375	electrostatic interaction	1351:1375	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	6	63	theme	electrostatic	1351:1363	arg1	interactions					1330:1341	interactions	1330:1341	interactions namely	1330:1348	The mechanism of MB and RR120 adsorption onto the CHS-ECH/ZL indicates various types of interactions namely, electrostatic interaction, hydrogen bonding, and Yoshida H-bonding in addition to n-π interaction.					
32634511	0	64	theme	blue	82:85	arg1	chitosan-epichlorohydrin/zeolite					21:52	Zwitterion composite chitosan-epichlorohydrin/zeolite	0:52	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.	0:112	Zwitterion composite chitosan-epichlorohydrin/zeolite for adsorption of methylene blue and reactive red 120 dyes.					
32634511	2	65	theme	composite	339:347	arg1	zwitterion					328:337	The zwitterion composite adsorbent	324:357	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL)	324:406	The zwitterion composite adsorbent of chitosan-epichlorohydrin/zeolite (CHS-ECH/ZL) is performed multifunctional tasks by removing two structurally different cationic (methylene blue dye, MB), and anionic (reactive red 120 dye, RR120) dyes from aqueous solutions.					
32634511	1	66	theme	covalent	233:240	arg1	cross-linker					242:253	a covalent cross-linker	231:253	a covalent cross-linker (epichlorohydrin, ECH)	231:276	In this research, an attempt to develop zwitterion composite adsorbent is conducted by modifying chitosan (CHS) with a covalent cross-linker (epichlorohydrin, ECH) and an aluminosilicate mineral (zeolite, ZL).					
33812193	0	0	theme	whole	61:65	arg1	meal					67:70	whole meal	61:70	whole meal	61:70	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	2	1	theme	quality	301:307	arg1	printing					309:316	a more accurate and better quality printing	274:316	a more accurate and better quality printing than wheat flour	274:333	Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour.					
33812193	6	2	theme	soft	799:802	arg1	texture					816:822	a soft and elastic texture	797:822	a soft and elastic texture after long-time storage	797:846	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	4	3	theme	meal	507:510	arg1	samples					474:480	the samples	470:480	the samples of wheat flour and whole meal	470:510	Starch-protein complexes appeared in the samples of wheat flour and whole meal.					
33812193	6	4	theme	elastic	808:814	arg1	texture					816:822	a soft and elastic texture	797:822	a soft and elastic texture after long-time storage	797:846	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	3	5	contain	had	348:350	arg2	capacity					372:379	the worst printable capacity	352:379	the worst printable capacity	352:379	Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer.					
33812193	3	5	contain	had	348:350	arg1	flour					342:346	Wheat flour	336:346	Wheat flour	336:346	Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer.					
33812193	2	6	theme	better	294:299	arg1	printing					309:316	a more accurate and better quality printing	274:316	a more accurate and better quality printing than wheat flour	274:333	Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour.					
33812193	4	7	theme	whole	501:505	arg1	meal					507:510	whole meal	501:510	whole meal	501:510	Starch-protein complexes appeared in the samples of wheat flour and whole meal.					
33812193	4	8	theme	Starch-protein	433:446	arg1	complexes					448:456	Starch-protein complexes	433:456	Starch-protein complexes	433:456	Starch-protein complexes appeared in the samples of wheat flour and whole meal.					
33812193	0	9	from	starch	43:48	arg1	performance					12:22	3D printing performance	0:22	3D printing performance of gels from wheat starch, flour and whole meal.	0:71	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	5	10	theme	various	575:581	arg1	complexes					583:591	various complexes	575:591	various complexes	575:591	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	2	11	theme	wheat	237:241	arg1	starch					243:248	wheat starch	237:248	wheat starch	237:248	Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour.					
33812193	5	12	theme	starch	649:654	arg1	aging					656:660	starch aging	649:660	starch aging	649:660	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	7	13	theme	whole	858:862	arg1	meal					864:867	whole meal	858:867	whole meal with higher protein, fat and fiber content	858:910	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	3	14	theme	printable	362:370	arg1	capacity					372:379	the worst printable capacity	352:379	the worst printable capacity	352:379	Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer.					
33812193	1	15	theme	food	138:141	arg1	quality					116:122	the quality	112:122	the quality of 3D-printed food using wheat starch, flour and whole meal	112:182	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	3	16	theme	Wheat	336:340	arg1	flour					342:346	Wheat flour	336:346	Wheat flour	336:346	Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer.					
33812193	0	17	theme	printing	3:10	arg1	performance					12:22	3D printing performance	0:22	3D printing performance of gels from wheat starch, flour and whole meal.	0:71	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	4	18	theme	flour	491:495	arg1	samples					474:480	the samples	470:480	the samples of wheat flour and whole meal	470:510	Starch-protein complexes appeared in the samples of wheat flour and whole meal.					
33812193	1	19	theme	wheat	149:153	arg1	starch					155:160	wheat starch	149:160	wheat starch	149:160	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	0	20	theme	3D	0:1	arg1	performance					12:22	3D printing performance	0:22	3D printing performance of gels from wheat starch, flour and whole meal.	0:71	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	4	21	theme	wheat	485:489	arg1	flour					491:495	wheat flour	485:495	wheat flour	485:495	Starch-protein complexes appeared in the samples of wheat flour and whole meal.					
33812193	2	22	theme	wheat	323:327	arg1	flour					329:333	wheat flour	323:333	wheat flour	323:333	Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour.					
33812193	7	23	theme	wheat	949:953	arg1	starch					955:960	wheat starch	949:960	wheat starch	949:960	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	5	24	theme	Whole	513:517	arg1	meal					519:522	Whole meal	513:522	Whole meal	513:522	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	2	25	theme	whole	254:258	arg1	meal					260:263	whole meal	254:263	whole meal	254:263	Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour.					
33812193	5	26	theme	only	737:740	arg1	sample					742:747	just the starch only sample	721:747	just the starch only sample	721:747	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	5	27	theme	starch	730:735	arg1	sample					742:747	just the starch only sample	721:747	just the starch only sample	721:747	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	5	28	theme	gel	615:617	arg1	cross-linking					627:639	the gel network cross-linking	611:639	the gel network cross-linking	611:639	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	1	29	theme	3D-printed	127:136	arg1	food					138:141	3D-printed food	127:141	3D-printed food using wheat starch, flour and whole meal	127:182	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	6	30	contain	possess	789:795	arg2	texture					816:822	a soft and elastic texture	797:822	a soft and elastic texture after long-time storage	797:846	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	6	30	contain	possess	789:795	arg1	samples					758:764	Printed samples	750:764	Printed samples using whole meal	750:781	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	3	31	theme	printer	424:430	arg1	wall					412:415	the wall	408:415	the wall of the printer	408:430	Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer.					
33812193	2	32	theme	accurate	281:288	arg1	printing					309:316	a more accurate and better quality printing	274:316	a more accurate and better quality printing than wheat flour	274:333	Results showed that samples molded by wheat starch and whole meal obtained a more accurate and better quality printing than wheat flour.					
33812193	5	33	theme	network	619:625	arg1	cross-linking					627:639	the gel network cross-linking	611:639	the gel network cross-linking	611:639	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	6	34	theme	whole	772:776	arg1	meal					778:781	whole meal	772:781	whole meal	772:781	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	0	35	theme	gels	27:30	arg1	performance					12:22	3D printing performance	0:22	3D printing performance of gels from wheat starch, flour and whole meal.	0:71	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	3	36	theme	worst	356:360	arg1	capacity					372:379	the worst printable capacity	352:379	the worst printable capacity	352:379	Wheat flour had the worst printable capacity as it gets stuck easily to the wall of the printer.					
33812193	0	37	from	flour	51:55	arg1	performance					12:22	3D printing performance	0:22	3D printing performance of gels from wheat starch, flour and whole meal.	0:71	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	1	38	theme	whole	173:177	arg1	meal					179:182	whole meal	173:182	whole meal	173:182	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	5	39	theme	binding	699:705	arg1	capacity					707:714	the water binding capacity	689:714	the water binding capacity than just the starch only sample	689:747	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	7	40	theme	food	927:930	arg1	printing					935:942	food 3D printing	927:942	food 3D printing	927:942	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	0	41	theme	wheat	37:41	arg1	starch					43:48	wheat starch	37:48	wheat starch	37:48	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	7	42	with	meal	864:867	arg1	fat					890:892	fat	890:892	fat	890:892	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	7	42	with	meal	864:867	arg1	content					904:910	fiber content	898:910	fiber content	898:910	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	7	42	with	meal	864:867	arg1	protein					881:887	higher protein	874:887	higher protein	874:887	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	7	43	theme	3D	932:933	arg1	printing					935:942	food 3D printing	927:942	food 3D printing	927:942	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	6	44	theme	Printed	750:756	arg1	samples					758:764	Printed samples	750:764	Printed samples using whole meal	750:781	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	1	45	theme	material	88:95	arg1	composition					97:107	material composition	88:107	material composition	88:107	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	6	46	theme	long-time	830:838	arg1	storage					840:846	long-time storage	830:846	long-time storage	830:846	Printed samples using whole meal still possess a soft and elastic texture after long-time storage.					
33812193	7	47	theme	fiber	898:902	arg1	content					904:910	fiber content	898:910	fiber content	898:910	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
33812193	1	48	theme	composition	97:107	arg1	effects					77:83	The effects	73:83	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal	73:182	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	1	49	from	effects	77:83	arg1	quality					116:122	the quality	112:122	the quality of 3D-printed food using wheat starch, flour and whole meal	112:182	The effects of material composition on the quality of 3D-printed food using wheat starch, flour and whole meal were explored.					
33812193	0	50	from	meal	67:70	arg1	performance					12:22	3D printing performance	0:22	3D printing performance of gels from wheat starch, flour and whole meal.	0:71	3D printing performance of gels from wheat starch, flour and whole meal.					
33812193	5	51	theme	water	693:697	arg1	capacity					707:714	the water binding capacity	689:714	the water binding capacity than just the starch only sample	689:747	Whole meal constituted protein, fat, fiber and starch to form various complexes, which strengthen the gel network cross-linking, retard starch aging significantly, and enhance the water binding capacity than just the starch only sample.					
33812193	7	52	theme	higher	874:879	arg1	protein					881:887	higher protein	874:887	higher protein	874:887	Overall, whole meal with higher protein, fat and fiber content was better for food 3D printing than wheat starch and flour.					
34478793	3	0	from	efficiency	441:450	arg1	solutions					494:502	high initial concentration solutions	467:502	high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively	467:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	3	1	theme	concentration	480:492	arg1	solutions					494:502	high initial concentration solutions	467:502	high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively	467:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	0	2	theme	synergistic	75:85	arg1	adsorption					87:96	synergistic adsorption	75:96	synergistic adsorption	75:96	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	3	3	dep	Cr	456:457	arg1	VI					460:461	VI	460:461	VI	460:461	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	4	4	theme	electrostatic	666:678	arg1	interaction					680:690	electrostatic interaction	666:690	electrostatic interaction	666:690	We discovered experimentally that the adsorption was dominated via electrostatic interaction while the blending of GO could contribute in stimulating electrons for the photoreduction process.					
34478793	4	5	theme	GO	714:715	arg1	blending					702:709	the blending	698:709	the blending of GO	698:715	We discovered experimentally that the adsorption was dominated via electrostatic interaction while the blending of GO could contribute in stimulating electrons for the photoreduction process.					
34478793	5	6	theme	active	922:927	arg1	sites					929:933	the active sites	918:933	the active sites of composite	918:946	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	5	6	theme	active	922:927	arg1	composite					938:946	composite	938:946	composite	938:946	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	6	7	theme	material	1088:1095	arg1	bandgap					1063:1069	the bandgap	1059:1069	the bandgap of the composite material	1059:1095	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	3	8	theme	removal	511:517	arg1	rate					519:522	the removal rate	507:522	the removal rate	507:522	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	6	9	dep	Cr	1169:1170	arg1	VI					1173:1174	VI	1173:1174	VI	1173:1174	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	3	10	theme	removal	433:439	arg1	efficiency					441:450	remarkable removal efficiency	422:450	remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively	422:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	4	11	theme	photoreduction	767:780	arg1	process					782:788	the photoreduction process	763:788	the photoreduction process	763:788	We discovered experimentally that the adsorption was dominated via electrostatic interaction while the blending of GO could contribute in stimulating electrons for the photoreduction process.					
34478793	6	12	theme	composite	1078:1086	arg1	material					1088:1095	the composite material	1074:1095	the composite material	1074:1095	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	6	13	dep	blending	1036:1043	arg1	GO					1045:1046	GO	1045:1046	GO	1045:1046	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	5	14	dep	regenerating	905:916	arg1	could					899:903	could	899:903	could	899:903	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	2	15	theme	aqueous	370:376	arg1	solutions					378:386	aqueous solutions	370:386	aqueous solutions	370:386	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	0	16	theme	Mechanistic	0:10	arg1	study					12:16	Mechanistic study	0:16	Mechanistic study of Cr (VI) removal by modified alginate/GO	0:59	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	3	17	dep	solutions	494:502	arg1	reached					524:530	reached	524:530	reached 86.16% and 99.92% for adsorption and photoreduction, respectively	524:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	3	18	theme	remarkable	422:431	arg1	efficiency					441:450	remarkable removal efficiency	422:450	remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively	422:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	0	19	theme	Cr	21:22	arg1	study					12:16	Mechanistic study	0:16	Mechanistic study of Cr (VI) removal by modified alginate/GO	0:59	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	5	20	theme	composite	938:946	arg1	sites					929:933	the active sites	918:933	the active sites of composite	918:946	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	5	20	theme	composite	938:946	arg1	composite					938:946	composite	938:946	composite	938:946	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	2	21	dep	Cr	356:357	arg1	VI					360:361	VI	360:361	VI	360:361	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	5	22	theme	surface	834:840	arg1	charge					842:847	the surface charge	830:847	the surface charge of chromium species	830:867	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	6	23	theme	light	1115:1119	arg1	response					1121:1128	the light response	1111:1128	the light response	1111:1128	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	0	24	theme	photocatalytic	102:115	arg1	reduction					117:125	photocatalytic reduction	102:125	photocatalytic reduction	102:125	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	6	25	theme	photoreduction	1138:1151	arg1	ability					1153:1159	the photoreduction ability	1134:1159	the photoreduction ability	1134:1159	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	6	26	theme	band	978:981	arg1	energy					983:988	The conduction band energy	963:988	The conduction band energy	963:988	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	1	27	theme	graphene	180:187	arg1	oxide					189:193	graphene oxide	180:193	graphene oxide	180:193	A novel composite material was prepared by blending graphene oxide into polyethyleneimine grafted sodium alginate.					
34478793	5	28	theme	species	861:867	arg1	charge					842:847	the surface charge	830:847	the surface charge of chromium species	830:867	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	6	29	theme	conduction	967:976	arg1	energy					983:988	The conduction band energy	963:988	The conduction band energy	963:988	The conduction band energy was calculated as -2.04 eV, which proved that blending GO can narrow the bandgap of the composite material, thus enhance the light response and the photoreduction ability towards Cr (VI).					
34478793	1	30	theme	grafted	218:224	arg1	alginate					233:240	polyethyleneimine grafted sodium alginate	200:240	polyethyleneimine grafted sodium alginate	200:240	A novel composite material was prepared by blending graphene oxide into polyethyleneimine grafted sodium alginate.					
34478793	3	31	theme	high	467:470	arg1	solutions					494:502	high initial concentration solutions	467:502	high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively	467:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	3	32	theme	composite	393:401	arg1	material					403:410	The composite material	389:410	The composite material	389:410	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	4	33	dep	discovered	602:611	arg1	dominated					652:660	dominated	652:660	was dominated via electrostatic interaction while the blending of GO could contribute in stimulating electrons for the photoreduction process	648:788	We discovered experimentally that the adsorption was dominated via electrostatic interaction while the blending of GO could contribute in stimulating electrons for the photoreduction process.					
34478793	3	34	theme	initial	472:478	arg1	solutions					494:502	high initial concentration solutions	467:502	high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively	467:596	The composite material has shown remarkable removal efficiency for Cr (VI) in high initial concentration solutions as the removal rate reached 86.16% and 99.92% for adsorption and photoreduction, respectively.					
34478793	0	35	dep	Cr	21:22	arg1	VI					25:26	VI	25:26	VI	25:26	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	0	35	dep	Cr	21:22	arg1	removal					29:35	removal	29:35	Cr (VI) removal by modified alginate/GO	21:59	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	2	36	from	solutions	378:386	arg1	removal					324:330	the removal	320:330	the removal of hexavalent chromium (Cr (VI)) from aqueous solutions	320:386	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	2	37	theme	chromium	346:353	arg1	removal					324:330	the removal	320:330	the removal of hexavalent chromium (Cr (VI)) from aqueous solutions	320:386	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	5	38	theme	electrostatic	875:887	arg1	repulsion					889:897	electrostatic repulsion	875:897	electrostatic repulsion	875:897	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	1	39	theme	sodium	226:231	arg1	alginate					233:240	polyethyleneimine grafted sodium alginate	200:240	polyethyleneimine grafted sodium alginate	200:240	A novel composite material was prepared by blending graphene oxide into polyethyleneimine grafted sodium alginate.					
34478793	1	40	dep	grafted	218:224	arg1	polyethyleneimine					200:216	polyethyleneimine	200:216	polyethyleneimine	200:216	A novel composite material was prepared by blending graphene oxide into polyethyleneimine grafted sodium alginate.					
34478793	2	41	theme	hexavalent	335:344	arg1	Cr					356:357	Cr (VI)	356:362	Cr (VI)	356:362	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	2	41	theme	hexavalent	335:344	arg1	chromium					346:353	hexavalent chromium	335:353	hexavalent chromium (Cr (VI))	335:363	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	0	42	theme	modified	40:47	arg1	alginate/GO					49:59	modified alginate/GO	40:59	modified alginate/GO	40:59	Mechanistic study of Cr (VI) removal by modified alginate/GO composite via synergistic adsorption and photocatalytic reduction.					
34478793	5	43	theme	chromium	852:859	arg1	species					861:867	chromium species	852:867	chromium species	852:867	Moreover, the photoreduction can alter the surface charge of chromium species, thus electrostatic repulsion could regenerating the active sites of composite spontaneously.					
34478793	1	44	theme	novel	130:134	arg1	material					146:153	A novel composite material	128:153	A novel composite material	128:153	A novel composite material was prepared by blending graphene oxide into polyethyleneimine grafted sodium alginate.					
34478793	2	45	theme	synthesized	247:257	arg1	material					259:266	The synthesized material	243:266	The synthesized material	243:266	The synthesized material was investigated as adsorbent and photocatalyst for the removal of hexavalent chromium (Cr (VI)) from aqueous solutions.					
34478793	1	46	theme	composite	136:144	arg1	material					146:153	A novel composite material	128:153	A novel composite material	128:153	A novel composite material was prepared by blending graphene oxide into polyethyleneimine grafted sodium alginate.					
34335628	7	0	theme	Prebiotic	1276:1284	arg1	supplementation					1286:1300	Prebiotic supplementation	1276:1300	Prebiotic supplementation	1276:1300	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	9	1	theme	prebiotic	1472:1480	arg1	supplementation					1482:1496	prebiotic supplementation	1472:1496	prebiotic supplementation during pregnancy	1472:1513	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	7	2	from	abundance	1316:1324	arg1	fetus					1388:1392	the fetus	1384:1392	the fetus	1384:1392	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	7	2	from	abundance	1316:1324	arg1	tissues					1369:1375	gestational tissues	1357:1375	gestational tissues	1357:1375	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	2	3	theme	compounds	355:363	arg1	production					332:341	the production	328:341	the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system	328:458	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	9	4	theme	immune	1567:1572	arg1	factors					1574:1580	specific microbial and immune factors	1544:1580	specific microbial and immune factors	1544:1580	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	9	5	from	mother	1587:1592	arg1	transmission					1528:1539	the transmission	1524:1539	the transmission of specific microbial and immune factors from mother to child	1524:1601	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	3	6	theme	short-chain	529:539	arg1	acids					547:551	short-chain fatty acids	529:551	short-chain fatty acids that can specifically interact with the immune system	529:605	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	9	7	from	establishment	1617:1629	arg1	fetus					1671:1675	the fetus	1667:1675	the fetus that may be beneficial for infant health outcomes	1667:1725	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	9	7	from	establishment	1617:1629	arg1	beneficial					1689:1698	beneficial	1689:1698	beneficial	1689:1698	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	0	8	theme	Amniotic	100:107	arg1	Fluid					109:113	Amniotic Fluid	100:113	Amniotic Fluid	100:113	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	6	9	theme	first	1246:1250	arg1	time					1252:1255	the first time	1242:1255	the first time	1242:1255	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	3	10	theme	fatty	541:545	arg1	acids					547:551	short-chain fatty acids	529:551	short-chain fatty acids that can specifically interact with the immune system	529:605	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	5	11	theme	healthy	970:976	arg1	imprinting					985:994	healthy immune imprinting	970:994	healthy immune imprinting	970:994	We hypothesized that galacto-oligosaccharides/inulin supplementation during gestation could modify the maternal microbiota, favoring healthy immune imprinting in the fetus.					
34335628	2	12	theme	specific	346:353	arg1	compounds					355:363	specific compounds	346:363	specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system	346:458	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	9	13	theme	specific	1544:1551	arg1	factors					1574:1580	specific microbial and immune factors	1544:1580	specific microbial and immune factors	1544:1580	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	2	14	theme	microbiota	280:289	arg1	composition					291:301	the gut microbiota composition	272:301	the gut microbiota composition	272:301	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	5	15	theme	galacto-oligosaccharides/inulin	858:888	arg1	supplementation					890:904	galacto-oligosaccharides/inulin supplementation	858:904	galacto-oligosaccharides/inulin supplementation during gestation	858:921	We hypothesized that galacto-oligosaccharides/inulin supplementation during gestation could modify the maternal microbiota, favoring healthy immune imprinting in the fetus.					
34335628	0	16	theme	Gut	56:58	arg1	Microbiota					60:69	Gut Microbiota	56:69	Gut Microbiota	56:69	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	5	17	theme	maternal	940:947	arg1	microbiota					949:958	the maternal microbiota	936:958	the maternal microbiota	936:958	We hypothesized that galacto-oligosaccharides/inulin supplementation during gestation could modify the maternal microbiota, favoring healthy immune imprinting in the fetus.					
34335628	2	18	theme	gut	276:278	arg1	composition					291:301	the gut microbiota composition	272:301	the gut microbiota composition	272:301	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	6	19	theme	gut	1156:1158	arg1	microbiota					1160:1169	the gut microbiota	1152:1169	the gut microbiota	1152:1169	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	2	20	theme	immune	446:451	arg1	system					453:458	the immune system	442:458	the immune system	442:458	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	9	21	theme	tolerogenic	1634:1644	arg1	imprinting					1653:1662	tolerogenic immune imprinting	1634:1662	tolerogenic immune imprinting	1634:1662	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	5	22	theme	immune	978:983	arg1	imprinting					985:994	healthy immune imprinting	970:994	healthy immune imprinting	970:994	We hypothesized that galacto-oligosaccharides/inulin supplementation during gestation could modify the maternal microbiota, favoring healthy immune imprinting in the fetus.					
34335628	7	23	theme	T	1346:1346	arg1	cells					1348:1352	regulatory B and T cells	1329:1352	regulatory B and T cells	1329:1352	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	0	24	theme	Prebiotic	0:8	arg1	Supplementation					10:24	Prebiotic Supplementation	0:24	Prebiotic Supplementation During Pregnancy	0:41	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	9	25	theme	immune	1646:1651	arg1	imprinting					1653:1662	tolerogenic immune imprinting	1634:1662	tolerogenic immune imprinting	1634:1662	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	9	26	theme	imprinting	1653:1662	arg1	establishment					1617:1629	the establishment	1613:1629	the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes	1613:1725	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	6	27	theme	Galacto-oligosaccharides/inulin	1010:1040	arg1	supplementation					1042:1056	Galacto-oligosaccharides/inulin supplementation	1010:1056	Galacto-oligosaccharides/inulin supplementation during gestation	1010:1073	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	0	28	theme	Tolerogenic	126:136	arg1	Environment					138:148	a Tolerogenic Environment	124:148	a Tolerogenic Environment In Utero	124:157	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	7	29	theme	regulatory	1329:1338	arg1	cells					1348:1352	regulatory B and T cells	1329:1352	regulatory B and T cells	1329:1352	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	3	30	theme	acids	547:551	arg1	release					518:524	the release	514:524	the release of short-chain fatty acids that can specifically interact with the immune system	514:605	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	1	31	theme	environmental	196:208	arg1	factors					210:216	environmental factors	196:216	environmental factors such as food	196:229	The gut microbiota is influenced by environmental factors such as food.					
34335628	1	31	theme	environmental	196:208	arg1	food					226:229	food	226:229	food	226:229	The gut microbiota is influenced by environmental factors such as food.					
34335628	3	32	theme	tolerogenic	633:643	arg1	populations					645:655	tolerogenic populations	633:655	tolerogenic populations	633:655	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	1	33	theme	gut	164:166	arg1	microbiota					168:177	The gut microbiota	160:177	The gut microbiota	160:177	The gut microbiota is influenced by environmental factors such as food.					
34335628	6	34	theme	increased	1183:1191	arg1	production					1193:1202	increased production	1183:1202	increased production	1183:1202	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	9	35	theme	factors	1574:1580	arg1	transmission					1528:1539	the transmission	1524:1539	the transmission of specific microbial and immune factors from mother to child	1524:1601	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	0	36	theme	In	150:151	arg1	Environment					138:148	a Tolerogenic Environment	124:148	a Tolerogenic Environment In Utero	124:157	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	0	37	from	Metabolites	85:95	arg1	Fluid					109:113	Amniotic Fluid	100:113	Amniotic Fluid	100:113	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	7	38	theme	gestational	1357:1367	arg1	tissues					1369:1375	gestational tissues	1357:1375	gestational tissues	1357:1375	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	9	39	theme	infant	1704:1709	arg1	outcomes					1718:1725	infant health outcomes	1704:1725	infant health outcomes	1704:1725	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	2	40	theme	Maternal	232:239	arg1	diet					241:244	Maternal diet	232:244	Maternal diet during pregnancy	232:261	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	0	41	theme	Microbiota	60:69	arg1	Metabolites					85:95	the Gut Microbiota and Increases Metabolites	52:95	the Gut Microbiota and Increases Metabolites in Amniotic Fluid	52:113	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	9	42	theme	microbial	1553:1561	arg1	factors					1574:1580	specific microbial and immune factors	1544:1580	specific microbial and immune factors	1544:1580	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	3	43	theme	gut	489:491	arg1	bacteria					493:500	gut bacteria	489:500	gut bacteria	489:500	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	2	44	theme	system	453:458	arg1	maturation					428:437	maturation	428:437	maturation	428:437	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	2	44	theme	system	453:458	arg1	ontogeny					415:422	ontogeny	415:422	ontogeny	415:422	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	2	45	dep	ontogeny	415:422	arg1	the					411:413	the	411:413	the	411:413	Maternal diet during pregnancy modifies the gut microbiota composition and function, leading to the production of specific compounds that are transferred to the fetus and enhance the ontogeny and maturation of the immune system.					
34335628	7	46	theme	cells	1348:1352	arg1	abundance					1316:1324	the abundance	1312:1324	the abundance of regulatory B and T cells in gestational tissues and in the fetus	1312:1392	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34335628	6	47	theme	fecal	1207:1211	arg1	acetate					1213:1219	fecal acetate	1207:1219	fecal acetate	1207:1219	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	6	48	theme	Bacteroidetes	1102:1114	arg1	abundance					1089:1097	the abundance	1085:1097	the abundance of Bacteroidetes	1085:1114	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	3	49	theme	health	682:687	arg1	benefits					689:696	health benefits	682:696	health benefits	682:696	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	3	50	theme	immune	593:598	arg1	system					600:605	the immune system	589:605	the immune system	589:605	Prebiotics are fermented by gut bacteria, leading to the release of short-chain fatty acids that can specifically interact with the immune system, inducing a switch toward tolerogenic populations and therefore conferring health benefits.					
34335628	4	51	theme	BALB/cJRj	723:731	arg1	mice					733:736	pregnant BALB/cJRj mice	714:736	pregnant BALB/cJRj mice	714:736	In this study, pregnant BALB/cJRj mice were fed either a control diet or a diet enriched in prebiotics (Galacto-oligosaccharides/Inulin).					
34335628	8	52	theme	regulatory	1416:1425	arg1	cells					1427:1431	these regulatory cells	1410:1431	these regulatory cells	1410:1431	Interestingly, these regulatory cells remained later in life.					
34335628	4	53	theme	control	756:762	arg1	diet					764:767	a control diet	754:767	a control diet	754:767	In this study, pregnant BALB/cJRj mice were fed either a control diet or a diet enriched in prebiotics (Galacto-oligosaccharides/Inulin).					
34335628	4	54	theme	pregnant	714:721	arg1	mice					733:736	pregnant BALB/cJRj mice	714:736	pregnant BALB/cJRj mice	714:736	In this study, pregnant BALB/cJRj mice were fed either a control diet or a diet enriched in prebiotics (Galacto-oligosaccharides/Inulin).					
34335628	6	55	theme	acetate	1213:1219	arg1	production					1193:1202	increased production	1183:1202	increased production	1183:1202	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	6	56	located	found	1232:1236	arg1	fluid					1269:1273	amniotic fluid	1260:1273	amniotic fluid	1260:1273	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	6	56	located	found	1232:1236	arg2	production					1193:1202	increased production	1183:1202	increased production	1183:1202	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	0	57	theme	Increases	75:83	arg1	Metabolites					85:95	the Gut Microbiota and Increases Metabolites	52:95	the Gut Microbiota and Increases Metabolites in Amniotic Fluid	52:113	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	6	58	theme	amniotic	1260:1267	arg1	fluid					1269:1273	amniotic fluid	1260:1273	amniotic fluid	1260:1273	Galacto-oligosaccharides/inulin supplementation during gestation increases the abundance of Bacteroidetes and decreases that of Firmicutes in the gut microbiota, leading to increased production of fecal acetate, which was found for the first time in amniotic fluid.					
34335628	0	59	dep	In	150:151	arg1	Utero					153:157	Utero	153:157	Utero	153:157	Prebiotic Supplementation During Pregnancy Modifies the Gut Microbiota and Increases Metabolites in Amniotic Fluid, Driving a Tolerogenic Environment In Utero.					
34335628	9	60	theme	health	1711:1716	arg1	outcomes					1718:1725	infant health outcomes	1704:1725	infant health outcomes	1704:1725	In conclusion, prebiotic supplementation during pregnancy leads to the transmission of specific microbial and immune factors from mother to child, allowing the establishment of tolerogenic immune imprinting in the fetus that may be beneficial for infant health outcomes.					
34335628	7	61	theme	B	1340:1340	arg1	cells					1348:1352	regulatory B and T cells	1329:1352	regulatory B and T cells	1329:1352	Prebiotic supplementation increased the abundance of regulatory B and T cells in gestational tissues and in the fetus.					
34839192	0	0	theme	volatile	72:79	arg1	compounds					81:89	volatile compounds	72:89	volatile compounds	72:89	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	2	1	theme	CK	490:491	arg1	group					494:498	the control (CK) group	477:498	the control (CK) group	477:498	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	2	theme	chitosan	583:590	arg1	T-CS					607:610	T-CS	607:610	T-CS	607:610	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	2	theme	chitosan	583:590	arg1	nanoemulsions					592:604	thymol chitosan nanoemulsions	576:604	thymol chitosan nanoemulsions (T-CS)	576:611	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	3	theme	pH	673:674	arg1	values					665:670	lower TVB-N values	653:670	lower TVB-N values	653:670	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	3	theme	pH	673:674	arg1	values					676:681	pH values	673:681	pH values	673:681	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	1	4	theme	volatile	247:254	arg1	compounds					256:264	the volatile compounds	243:264	the volatile compounds	243:264	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	6	5	theme	spoilage	1256:1263	arg1	organisms					1265:1273	spoilage organisms	1256:1273	spoilage organisms	1256:1273	By contrast, both coatings changed the microbial composition, reduced the proportion of spoilage organisms and retained bacterial diversity.					
34839192	2	6	theme	thymol	576:581	arg1	T-CS					607:610	T-CS	607:610	T-CS	607:610	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	6	theme	thymol	576:581	arg1	nanoemulsions					592:604	thymol chitosan nanoemulsions	576:604	thymol chitosan nanoemulsions (T-CS)	576:611	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	1	7	theme	refrigerated	443:454	arg1	pork					456:459	refrigerated pork	443:459	refrigerated pork	443:459	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	0	8	from	Impacts	0:6	arg1	compounds					81:89	volatile compounds	72:89	volatile compounds	72:89	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	0	8	from	Impacts	0:6	arg1	diversity					105:113	microbial diversity	95:113	microbial diversity of refrigerated pork meat	95:139	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	1	9	theme	pork	456:459	arg1	components					405:414	volatile components	396:414	volatile components	396:414	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	1	9	theme	pork	456:459	arg1	diversity					430:438	bacterial diversity	420:438	bacterial diversity of refrigerated pork	420:459	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	5	10	theme	main	1151:1154	arg1	microbiota					1156:1165	the main microbiota	1147:1165	the main microbiota	1147:1165	At day 12, Pseudomonas (84.3%) increased rapidly in the CK group and became the main microbiota.					
34839192	2	11	theme	treatment	505:513	arg1	groups					515:520	the treatment groups	501:520	the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS))	501:644	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	12	theme	chitosan	617:624	arg1	nanoemulsions					626:638	chitosan nanoemulsions	617:638	chitosan nanoemulsions (CS)	617:643	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	12	theme	chitosan	617:624	arg1	CS					641:642	CS	641:642	CS	641:642	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	3	13	theme	TEO-CS	863:868	arg1	groups					870:875	the T-CS and TEO-CS groups	850:875	the T-CS and TEO-CS groups	850:875	The E-nose and GC-MS evaluation indicated that compounds causing unpleasant odors could be inhibited in the T-CS and TEO-CS groups.					
34839192	7	14	theme	fresh	1471:1475	arg1	pork					1477:1480	fresh pork	1471:1480	fresh pork	1471:1480	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	4	15	theme	early	1049:1053	arg1	period					1063:1068	the early storage period	1045:1068	the early storage period	1045:1068	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	6	16	theme	organisms	1265:1273	arg1	proportion					1242:1251	the proportion	1238:1251	the proportion of spoilage organisms	1238:1273	By contrast, both coatings changed the microbial composition, reduced the proportion of spoilage organisms and retained bacterial diversity.					
34839192	0	17	theme	microbial	95:103	arg1	diversity					105:113	microbial diversity	95:113	microbial diversity of refrigerated pork meat	95:139	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	7	18	theme	antibacterial	1348:1360	arg1	substance					1362:1370	antibacterial substance	1348:1370	antibacterial substance	1348:1370	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	19	theme	pork	1477:1480	arg1	effect					1461:1466	the preservation effect	1444:1466	the preservation effect of fresh pork, which provides a solution to against conventional packaging	1444:1541	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	20	theme	chitosan	1320:1327	arg1	method					1426:1431	an effective supplementary and method	1395:1431	method	1426:1431	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	20	theme	chitosan	1320:1327	arg1	supplementary					1408:1420	an effective supplementary and method	1395:1431	supplementary	1408:1420	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	20	theme	chitosan	1320:1327	arg1	nanoemulsions					1329:1341	chitosan nanoemulsions	1320:1341	chitosan nanoemulsions with antibacterial substance	1320:1370	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	3	21	theme	T-CS	854:857	arg1	groups					870:875	the T-CS and TEO-CS groups	850:875	the T-CS and TEO-CS groups	850:875	The E-nose and GC-MS evaluation indicated that compounds causing unpleasant odors could be inhibited in the T-CS and TEO-CS groups.					
34839192	4	22	theme	storage	1055:1061	arg1	period					1063:1068	the early storage period	1045:1068	the early storage period	1045:1068	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	2	23	theme	thyme	523:527	arg1	nanoemulsions					552:564	thyme essential oil chitosan nanoemulsions	523:564	thyme essential oil chitosan nanoemulsions (TEO-CS)	523:573	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	23	theme	thyme	523:527	arg1	TEO-CS					567:572	TEO-CS	567:572	TEO-CS	567:572	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	1	24	theme	bacterial	274:282	arg1	composition					294:304	the bacterial community composition	270:304	the bacterial community composition of refrigerated pork stored at 4 °C for 12 days	270:352	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	7	25	theme	effective	1398:1406	arg1	supplementary					1408:1420	an effective supplementary and method	1395:1431	supplementary	1408:1420	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	25	theme	effective	1398:1406	arg1	nanoemulsions					1329:1341	chitosan nanoemulsions	1320:1341	chitosan nanoemulsions with antibacterial substance	1320:1370	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	4	26	theme	refrigerated	1024:1035	arg1	pork					1037:1040	refrigerated pork	1024:1040	refrigerated pork	1024:1040	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	4	27	theme	predominant	1002:1012	arg1	genera					1014:1019	predominant genera	1002:1019	predominant genera of refrigerated pork	1002:1040	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	0	28	theme	nanoemulsions	20:32	arg1	Impacts					0:6	Impacts	0:6	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.	0:140	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	1	29	theme	community	284:292	arg1	composition					294:304	the bacterial community composition	270:304	the bacterial community composition of refrigerated pork stored at 4 °C for 12 days	270:352	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	0	30	theme	refrigerated	118:129	arg1	meat					136:139	refrigerated pork meat	118:139	refrigerated pork meat	118:139	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	2	31	theme	chitosan	543:550	arg1	nanoemulsions					552:564	thyme essential oil chitosan nanoemulsions	523:564	thyme essential oil chitosan nanoemulsions (TEO-CS)	523:573	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	31	theme	chitosan	543:550	arg1	TEO-CS					567:572	TEO-CS	567:572	TEO-CS	567:572	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	0	32	theme	chitosan	11:18	arg1	nanoemulsions					20:32	chitosan nanoemulsions	11:32	chitosan nanoemulsions with thymol or thyme essential oil	11:67	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	2	33	theme	essential	529:537	arg1	nanoemulsions					552:564	thyme essential oil chitosan nanoemulsions	523:564	thyme essential oil chitosan nanoemulsions (TEO-CS)	523:573	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	33	theme	essential	529:537	arg1	TEO-CS					567:572	TEO-CS	567:572	TEO-CS	567:572	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	7	34	theme	preservation	1448:1459	arg1	effect					1461:1466	the preservation effect	1444:1466	the preservation effect of fresh pork, which provides a solution to against conventional packaging	1444:1541	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	35	theme	meat	1572:1575	arg1	shelf-life					1558:1567	the shelf-life	1554:1567	the shelf-life of meat	1554:1575	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	2	36	theme	color	727:731	arg1	degradation					733:743	color degradation	727:743	color degradation	727:743	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	1	37	theme	antibacterial	189:201	arg1	substances					203:212	antibacterial substances	189:212	antibacterial substances embedded in nanoemulsions	189:238	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	2	38	theme	control	481:487	arg1	group					494:498	the control (CK) group	477:498	the control (CK) group	477:498	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	0	39	theme	meat	136:139	arg1	compounds					81:89	volatile compounds	72:89	volatile compounds	72:89	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	0	39	theme	meat	136:139	arg1	diversity					105:113	microbial diversity	95:113	microbial diversity of refrigerated pork meat	95:139	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	4	40	theme	pork	1037:1040	arg1	genera					1014:1019	predominant genera	1002:1019	predominant genera of refrigerated pork	1002:1040	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	1	41	theme	substances	203:212	arg1	effects					178:184	the effects	174:184	the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days	174:352	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	1	42	theme	refrigerated	309:320	arg1	pork					322:325	refrigerated pork	309:325	refrigerated pork stored at 4 °C for 12 days	309:352	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	0	43	theme	pork	131:134	arg1	meat					136:139	refrigerated pork meat	118:139	refrigerated pork meat	118:139	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	1	44	theme	volatile	396:403	arg1	components					405:414	volatile components	396:414	volatile components	396:414	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	0	45	with	nanoemulsions	20:32	arg1	thymol					39:44	thymol	39:44	thymol	39:44	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	0	45	with	nanoemulsions	20:32	arg1	thyme					49:53	thyme	49:53	thyme	49:53	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	1	46	theme	embedded	214:221	arg1	substances					203:212	antibacterial substances	189:212	antibacterial substances embedded in nanoemulsions	189:238	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	2	47	dep	groups	515:520	arg1	nanoemulsions					626:638	chitosan nanoemulsions	617:638	chitosan nanoemulsions (CS)	617:643	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	47	dep	groups	515:520	arg1	nanoemulsions					552:564	thyme essential oil chitosan nanoemulsions	523:564	thyme essential oil chitosan nanoemulsions (TEO-CS)	523:573	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	47	dep	groups	515:520	arg1	nanoemulsions					592:604	thymol chitosan nanoemulsions	576:604	thymol chitosan nanoemulsions (T-CS)	576:611	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	47	dep	groups	515:520	arg1	TEO-CS					567:572	TEO-CS	567:572	TEO-CS	567:572	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	47	dep	groups	515:520	arg1	T-CS					607:610	T-CS	607:610	T-CS	607:610	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	47	dep	groups	515:520	arg1	CS					641:642	CS	641:642	CS	641:642	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	1	48	theme	pork	322:325	arg1	composition					294:304	the bacterial community composition	270:304	the bacterial community composition of refrigerated pork stored at 4 °C for 12 days	270:352	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	1	48	theme	pork	322:325	arg1	compounds					256:264	the volatile compounds	243:264	the volatile compounds	243:264	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	6	49	theme	microbial	1207:1215	arg1	composition					1217:1227	the microbial composition	1203:1227	the microbial composition	1203:1227	By contrast, both coatings changed the microbial composition, reduced the proportion of spoilage organisms and retained bacterial diversity.					
34839192	0	50	dep	thymol	39:44	arg1	oil					65:67	essential oil	55:67	essential oil	55:67	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	1	51	from	embedded	214:221	arg1	nanoemulsions					226:238	nanoemulsions	226:238	nanoemulsions	226:238	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	4	52	from	genera	1014:1019	arg1	period					1063:1068	the early storage period	1045:1068	the early storage period	1045:1068	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	6	53	theme	bacterial	1288:1296	arg1	diversity					1298:1306	bacterial diversity	1288:1306	bacterial diversity	1288:1306	By contrast, both coatings changed the microbial composition, reduced the proportion of spoilage organisms and retained bacterial diversity.					
34839192	7	54	with	nanoemulsions	1329:1341	arg1	substance					1362:1370	antibacterial substance	1348:1370	antibacterial substance	1348:1370	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	2	55	theme	oil	539:541	arg1	nanoemulsions					552:564	thyme essential oil chitosan nanoemulsions	523:564	thyme essential oil chitosan nanoemulsions (TEO-CS)	523:573	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	55	theme	oil	539:541	arg1	TEO-CS					567:572	TEO-CS	567:572	TEO-CS	567:572	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	56	theme	TVB-N	659:663	arg1	values					665:670	lower TVB-N values	653:670	lower TVB-N values	653:670	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	56	theme	TVB-N	659:663	arg1	values					676:681	pH values	673:681	pH values	673:681	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	56	theme	TVB-N	659:663	arg1	values					690:695	TBARs values	684:695	TBARs values	684:695	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	7	57	dep	supplementary	1408:1420	arg1	extend					1547:1552	extend	1547:1552	extend the shelf-life of meat	1547:1575	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	7	57	dep	supplementary	1408:1420	arg1	improve					1436:1442	improve	1436:1442	improve the preservation effect of fresh pork, which provides a solution to against conventional packaging	1436:1541	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	1	58	theme	bacterial	420:428	arg1	diversity					430:438	bacterial diversity	420:438	bacterial diversity of refrigerated pork	420:459	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	2	59	theme	lower	653:657	arg1	values					665:670	lower TVB-N values	653:670	lower TVB-N values	653:670	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	59	theme	lower	653:657	arg1	values					676:681	pH values	673:681	pH values	673:681	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	59	theme	lower	653:657	arg1	values					690:695	TBARs values	684:695	TBARs values	684:695	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	1	60	from	nanoemulsions	226:238	arg1	embedded					214:221	embedded	214:221	embedded	214:221	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	2	61	theme	TBARs	684:688	arg1	values					665:670	lower TVB-N values	653:670	lower TVB-N values	653:670	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	2	61	theme	TBARs	684:688	arg1	values					690:695	TBARs values	684:695	TBARs values	684:695	As compared to the control (CK) group, the treatment groups (thyme essential oil chitosan nanoemulsions (TEO-CS), thymol chitosan nanoemulsions (T-CS) and chitosan nanoemulsions (CS)) showed lower TVB-N values, pH values, TBARs values and better protective against color degradation.					
34839192	4	62	theme	high-throughput	891:905	arg1	sequencing					907:916	high-throughput sequencing	891:916	high-throughput sequencing	891:916	In addition, high-throughput sequencing showed that Pseudomonas (18.3%), Lactococcus (27.0%) and Acinetobacter (38.8%) were predominant genera of refrigerated pork in the early storage period.					
34839192	3	63	theme	GC-MS	761:765	arg1	evaluation					767:776	The E-nose and GC-MS evaluation	746:776	The E-nose and GC-MS evaluation	746:776	The E-nose and GC-MS evaluation indicated that compounds causing unpleasant odors could be inhibited in the T-CS and TEO-CS groups.					
34839192	1	64	from	effects	178:184	arg1	composition					294:304	the bacterial community composition	270:304	the bacterial community composition of refrigerated pork stored at 4 °C for 12 days	270:352	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	1	64	from	effects	178:184	arg1	compounds					256:264	the volatile compounds	243:264	the volatile compounds	243:264	This study aimed to investigate the effects of antibacterial substances embedded in nanoemulsions on the volatile compounds and the bacterial community composition of refrigerated pork stored at 4 °C for 12 days, and to evaluate the relationship between volatile components and bacterial diversity of refrigerated pork.					
34839192	3	65	theme	unpleasant	811:820	arg1	odors					822:826	unpleasant odors	811:826	unpleasant odors	811:826	The E-nose and GC-MS evaluation indicated that compounds causing unpleasant odors could be inhibited in the T-CS and TEO-CS groups.					
34839192	5	66	theme	CK	1127:1128	arg1	group					1130:1134	the CK group	1123:1134	the CK group	1123:1134	At day 12, Pseudomonas (84.3%) increased rapidly in the CK group and became the main microbiota.					
34839192	0	67	theme	essential	55:63	arg1	oil					65:67	essential oil	55:67	essential oil	55:67	Impacts of chitosan nanoemulsions with thymol or thyme essential oil on volatile compounds and microbial diversity of refrigerated pork meat.					
34839192	7	68	theme	conventional	1520:1531	arg1	packaging					1533:1541	conventional packaging	1520:1541	conventional packaging	1520:1541	Therefore, chitosan nanoemulsions with antibacterial substance could be considered as an effective supplementary and method to improve the preservation effect of fresh pork, which provides a solution to against conventional packaging and extend the shelf-life of meat.					
34839192	3	69	theme	E-nose	750:755	arg1	evaluation					767:776	The E-nose and GC-MS evaluation	746:776	The E-nose and GC-MS evaluation	746:776	The E-nose and GC-MS evaluation indicated that compounds causing unpleasant odors could be inhibited in the T-CS and TEO-CS groups.					
34128369	5	0	theme	kidney	1049:1054	arg1	cells					1071:1075	human embryonic kidney 293T (HEK293T) cells	1033:1075	human embryonic kidney 293T (HEK293T) cells	1033:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	2	1	theme	acid	466:469	arg1	hydrolysis					471:480	acid hydrolysis	466:480	acid hydrolysis	466:480	Cellulose nanofibers were obtained by two methods, based on either alkaline or acid hydrolysis.					
34128369	7	2	theme	renewable	1380:1388	arg1	potential					1361:1369	high potential	1356:1369	high potential	1356:1369	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	2	theme	renewable	1380:1388	arg1	source					1396:1401	a new renewable green source	1374:1401	a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications	1374:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	6	3	theme	concentration	1208:1220	arg1	µg/mL					1251:1255	0‍‒‍500 µg/mL	1243:1255	0‍‒‍500 µg/mL	1243:1255	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	6	3	theme	concentration	1208:1220	arg1	range					1222:1226	the concentration range	1204:1226	the concentration range assayed (i.e., 0‍‒‍500 µg/mL)	1204:1256	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	5	4	theme	nanofibers	1019:1028	arg1	effects					985:991	the cytotoxic effects	971:991	the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells	971:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	6	5	dep	µg/mL	1251:1255	arg1	i.e.					1237:1240	i.e.	1237:1240	i.e.	1237:1240	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	3	6	theme	energy-dispersive	636:652	arg1	EDX					674:676	EDX	674:676	EDX	674:676	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	6	theme	energy-dispersive	636:652	arg1	spectroscopy					660:671	energy-dispersive X-ray spectroscopy	636:671	energy-dispersive X-ray spectroscopy (EDX)	636:677	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	7	theme	dynamic	680:686	arg1	DLS					706:708	DLS	706:708	DLS	706:708	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	7	theme	dynamic	680:686	arg1	scattering					694:703	dynamic light scattering	680:703	dynamic light scattering (DLS)	680:709	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	4	8	theme	cellulose	828:836	arg1	nanofibers					838:847	the cellulose nanofibers	824:847	the cellulose nanofibers extracted from A. americana and R. communis	824:891	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	6	9	from	cytocompatible	1186:1199	arg1	µg/mL					1251:1255	0‍‒‍500 µg/mL	1243:1255	0‍‒‍500 µg/mL	1243:1255	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	6	9	from	cytocompatible	1186:1199	arg1	range					1222:1226	the concentration range	1204:1226	the concentration range assayed (i.e., 0‍‒‍500 µg/mL)	1204:1256	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	4	10	dep	R.	881:882	arg1	communis					884:891	R. communis	881:891	R. communis	881:891	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	1	11	theme	cytocompatible	327:340	arg1	nanofibers					375:384	cytocompatible and highly crystalline cellulose nanofibers	327:384	cytocompatible and highly crystalline cellulose nanofibers	327:384	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	7	12	theme	high	1356:1359	arg1	potential					1361:1369	high potential	1356:1369	high potential	1356:1369	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	12	theme	high	1356:1359	arg1	source					1396:1401	a new renewable green source	1374:1401	a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications	1374:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	13	theme	new	1376:1378	arg1	potential					1361:1369	high potential	1356:1369	high potential	1356:1369	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	13	theme	new	1376:1378	arg1	source					1396:1401	a new renewable green source	1374:1401	a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications	1374:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	1	14	used	used	277:280	arg2	fibers					189:194	the fibers	185:194	the fibers of invasive species Agave americana L. and Ricinus communis L.	185:257	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	1	14	used	used	277:280	arg2	sources					308:314	new sources	304:314	new sources	304:314	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	7	15	theme	communis	1336:1343	arg1	fibers					1345:1350	communis fibers	1336:1350	communis fibers	1336:1350	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	2	16	theme	Cellulose	387:395	arg1	nanofibers					397:406	Cellulose nanofibers	387:406	Cellulose nanofibers	387:406	Cellulose nanofibers were obtained by two methods, based on either alkaline or acid hydrolysis.					
34128369	5	17	theme	embryonic	1039:1047	arg1	kidney					1049:1054	human embryonic kidney 293T	1033:1059	human embryonic kidney 293T (HEK293T) cells	1033:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	5	17	theme	embryonic	1039:1047	arg1	HEK293T					1062:1068	HEK293T	1062:1068	HEK293T	1062:1068	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	4	18	theme	crystallinity	793:805	arg1	high					903:906	high	903:906	high	903:906	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	18	theme	crystallinity	793:805	arg1	%					913:913	94.1%	909:913	94.1%	909:913	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	18	theme	crystallinity	793:805	arg1	%					923:923	92.7%	919:923	92.7%	919:923	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	18	theme	crystallinity	793:805	arg1	CIs					816:818	CIs	816:818	CIs	816:818	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	18	theme	crystallinity	793:805	arg1	indexes					807:813	The crystallinity indexes	789:813	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis	789:891	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	6	19	dep	assayed	1228:1234	arg1	µg/mL					1251:1255	0‍‒‍500 µg/mL	1243:1255	0‍‒‍500 µg/mL	1243:1255	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	6	19	dep	assayed	1228:1234	arg1	range					1222:1226	the concentration range	1204:1226	the concentration range assayed (i.e., 0‍‒‍500 µg/mL)	1204:1256	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	5	20	theme	human	1033:1037	arg1	kidney					1049:1054	human embryonic kidney 293T	1033:1059	human embryonic kidney 293T (HEK293T) cells	1033:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	5	20	theme	human	1033:1037	arg1	HEK293T					1062:1068	HEK293T	1062:1068	HEK293T	1062:1068	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	0	21	theme	cellulose	15:23	arg1	nanofibers					25:34	Cytocompatible cellulose nanofibers	0:34	Cytocompatible cellulose nanofibers from invasive plant species	0:62	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	1	22	theme	crystalline	353:363	arg1	nanofibers					375:384	cytocompatible and highly crystalline cellulose nanofibers	327:384	cytocompatible and highly crystalline cellulose nanofibers	327:384	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	3	23	theme	materials	555:563	arg1	crystallinity					525:537	crystallinity	525:537	crystallinity	525:537	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	23	theme	materials	555:563	arg1	morphology					487:496	The morphology	483:496	The morphology	483:496	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	23	theme	materials	555:563	arg1	composition					508:518	chemical composition	499:518	chemical composition	499:518	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	0	24	from	species	56:62	arg1	nanofibers					25:34	Cytocompatible cellulose nanofibers	0:34	Cytocompatible cellulose nanofibers from invasive plant species	0:62	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	0	25	theme	Cytocompatible	0:13	arg1	nanofibers					25:34	Cytocompatible cellulose nanofibers	0:34	Cytocompatible cellulose nanofibers from invasive plant species	0:62	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	3	26	dep	infrared	759:766	arg1	FTIR					769:772	FTIR	769:772	FTIR	769:772	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	0	27	theme	green	120:124	arg1	source					126:131	a renewable green source	108:131	a renewable green source of highly crystalline nanocellulose	108:167	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	7	28	contain	has	1352:1354	arg1	nanocellulose					1287:1299	the nanocellulose	1283:1299	the nanocellulose extracted from A. americana and R. communis fibers	1283:1350	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	28	contain	has	1352:1354	arg2	source					1396:1401	a new renewable green source	1374:1401	a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications	1374:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	28	contain	has	1352:1354	arg2	potential					1361:1369	high potential	1356:1369	high potential	1356:1369	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	0	29	dep	L.	80:81	arg1	L.					104:105	L.	104:105	L.	104:105	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	3	30	theme	scanning	587:594	arg1	SEM					617:619	SEM	617:619	SEM	617:619	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	30	theme	scanning	587:594	arg1	microscopy					605:614	scanning electron microscopy	587:614	scanning electron microscopy (SEM)	587:620	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	31	theme	X-ray	712:716	arg1	XRD					731:733	XRD	731:733	XRD	731:733	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	31	theme	X-ray	712:716	arg1	diffraction					718:728	X-ray diffraction	712:728	X-ray diffraction (XRD)	712:734	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	0	32	theme	renewable	110:118	arg1	source					126:131	a renewable green source	108:131	a renewable green source of highly crystalline nanocellulose	108:167	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	1	33	theme	cellulose	365:373	arg1	nanofibers					375:384	cytocompatible and highly crystalline cellulose nanofibers	327:384	cytocompatible and highly crystalline cellulose nanofibers	327:384	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	3	34	theme	obtained	546:553	arg1	materials					555:563	the obtained materials	542:563	the obtained materials	542:563	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	0	35	theme	plant	50:54	arg1	species					56:62	invasive plant species	41:62	invasive plant species	41:62	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	7	36	theme	biomedical	1474:1483	arg1	applications					1485:1496	biomedical applications	1474:1496	biomedical applications	1474:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	3	37	theme	X-ray	654:658	arg1	EDX					674:676	EDX	674:676	EDX	674:676	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	37	theme	X-ray	654:658	arg1	spectroscopy					660:671	energy-dispersive X-ray spectroscopy	636:671	energy-dispersive X-ray spectroscopy (EDX)	636:677	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	0	38	dep	Agave	64:68	arg1	source					126:131	a renewable green source	108:131	a renewable green source of highly crystalline nanocellulose	108:167	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	7	39	theme	cytocompatible	1441:1454	arg1	nanomaterials					1456:1468	highly crystalline cellulose-based cytocompatible nanomaterials	1406:1468	highly crystalline cellulose-based cytocompatible nanomaterials	1406:1468	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	0	40	theme	invasive	41:48	arg1	species					56:62	invasive plant species	41:62	invasive plant species	41:62	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	1	41	dep	species	208:214	arg1	Agave					216:220	Agave	216:220	Agave	216:220	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	1	41	dep	species	208:214	arg1	L.					256:257	Ricinus communis L.	239:257	Ricinus communis L.	239:257	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	3	42	theme	electron	596:603	arg1	SEM					617:619	SEM	617:619	SEM	617:619	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	42	theme	electron	596:603	arg1	microscopy					605:614	scanning electron microscopy	587:614	scanning electron microscopy (SEM)	587:620	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	1	43	theme	invasive	199:206	arg1	species					208:214	invasive species	199:214	invasive species Agave americana L. and Ricinus communis L.	199:257	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	1	44	theme	first	290:294	arg1	time					296:299	the first time	286:299	the first time	286:299	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	0	45	theme	crystalline	143:153	arg1	nanocellulose					155:167	highly crystalline nanocellulose	136:167	highly crystalline nanocellulose	136:167	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	1	46	dep	Agave	216:220	arg1	L.					232:233	Agave americana L.	216:233	Agave americana L.	216:233	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	7	47	theme	green	1390:1394	arg1	potential					1361:1369	high potential	1356:1369	high potential	1356:1369	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	47	theme	green	1390:1394	arg1	source					1396:1401	a new renewable green source	1374:1401	a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications	1374:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	3	48	theme	light	688:692	arg1	DLS					706:708	DLS	706:708	DLS	706:708	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	48	theme	light	688:692	arg1	scattering					694:703	dynamic light scattering	680:703	dynamic light scattering (DLS)	680:709	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	7	49	theme	cellulose-based	1425:1439	arg1	nanomaterials					1456:1468	highly crystalline cellulose-based cytocompatible nanomaterials	1406:1468	highly crystalline cellulose-based cytocompatible nanomaterials	1406:1468	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	6	50	theme	extraction	1146:1155	arg1	methods					1157:1163	the two different extraction methods	1128:1163	the two different extraction methods	1128:1163	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	5	51	theme	cellulose	1009:1017	arg1	nanofibers					1019:1028	the prepared cellulose nanofibers	996:1028	the prepared cellulose nanofibers	996:1028	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	4	52	theme	nanofibers	838:847	arg1	high					903:906	high	903:906	high	903:906	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	52	theme	nanofibers	838:847	arg1	%					913:913	94.1%	909:913	94.1%	909:913	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	52	theme	nanofibers	838:847	arg1	%					923:923	92.7%	919:923	92.7%	919:923	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	52	theme	nanofibers	838:847	arg1	CIs					816:818	CIs	816:818	CIs	816:818	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	52	theme	nanofibers	838:847	arg1	indexes					807:813	The crystallinity indexes	789:813	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis	789:891	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	5	53	theme	cytotoxic	975:983	arg1	effects					985:991	the cytotoxic effects	971:991	the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells	971:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	6	54	theme	different	1136:1144	arg1	methods					1157:1163	the two different extraction methods	1128:1163	the two different extraction methods	1128:1163	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	5	55	theme	Biological	941:950	arg1	studies					952:958	Biological studies	941:958	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells	941:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	1	56	theme	species	208:214	arg1	fibers					189:194	the fibers	185:194	the fibers of invasive species Agave americana L. and Ricinus communis L.	185:257	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	1	56	theme	species	208:214	arg1	sources					308:314	new sources	304:314	new sources	304:314	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	0	57	theme	nanocellulose	155:167	arg1	source					126:131	a renewable green source	108:131	a renewable green source of highly crystalline nanocellulose	108:167	Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.					
34128369	5	58	theme	prepared	1000:1007	arg1	nanofibers					1019:1028	the prepared cellulose nanofibers	996:1028	the prepared cellulose nanofibers	996:1028	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	7	59	dep	americana	1319:1327	arg1	fibers					1345:1350	communis fibers	1336:1350	communis fibers	1336:1350	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	5	60	from	effects	985:991	arg1	cells					1071:1075	human embryonic kidney 293T (HEK293T) cells	1033:1075	human embryonic kidney 293T (HEK293T) cells	1033:1075	Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed.					
34128369	7	61	theme	crystalline	1413:1423	arg1	nanomaterials					1456:1468	highly crystalline cellulose-based cytocompatible nanomaterials	1406:1468	highly crystalline cellulose-based cytocompatible nanomaterials	1406:1468	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	4	62	dep	high	903:906	arg1	high					903:906	high	903:906	high	903:906	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	62	dep	high	903:906	arg1	%					913:913	94.1%	909:913	94.1%	909:913	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	62	dep	high	903:906	arg1	%					923:923	92.7%	919:923	92.7%	919:923	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	4	62	dep	high	903:906	arg1	indexes					807:813	The crystallinity indexes	789:813	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis	789:891	The crystallinity indexes (CIs) of the cellulose nanofibers extracted from A. americana and R. communis were very high (94.1% and 92.7%, respectively).					
34128369	3	63	theme	chemical	499:506	arg1	composition					508:518	chemical composition	499:518	chemical composition	499:518	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	6	64	from	range	1222:1226	arg1	cytocompatible					1186:1199	cytocompatible	1186:1199	cytocompatible	1186:1199	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	6	64	from	range	1222:1226	arg1	nanofibers					1102:1111	The nanofibers	1098:1111	The nanofibers obtained using the two different extraction methods	1098:1163	The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‍‒‍500 µg/mL).					
34128369	1	65	theme	new	304:306	arg1	fibers					189:194	the fibers	185:194	the fibers of invasive species Agave americana L. and Ricinus communis L.	185:257	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	1	65	theme	new	304:306	arg1	sources					308:314	new sources	304:314	new sources	304:314	In this study, the fibers of invasive species Agave americana L. and Ricinus communis L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers.					
34128369	7	66	theme	nanomaterials	1456:1468	arg1	potential					1361:1369	high potential	1356:1369	high potential	1356:1369	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	7	66	theme	nanomaterials	1456:1468	arg1	source					1396:1401	a new renewable green source	1374:1401	a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications	1374:1496	Our results showed that the nanocellulose extracted from A. americana and R. communis fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.					
34128369	3	67	dep	together	622:629	arg1	with					631:634	with	631:634	with	631:634	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
34128369	3	68	theme	infrared	759:766	arg1	spectroscopy					775:786	infrared (FTIR) spectroscopy	759:786	infrared (FTIR) spectroscopy	759:786	The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.					
32553962	10	0	theme	dye	1495:1497	arg1	removal					1499:1505	dye removal	1495:1505	dye removal	1495:1505	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	6	1	theme	pseudo-second-order	1007:1025	arg1	model					1035:1039	the pseudo-second-order kinetic model	1003:1039	the pseudo-second-order kinetic model	1003:1039	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	2	2	theme	Blue	574:577	arg1	AB158					584:588	AB158	584:588	AB158	584:588	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	2	theme	Blue	574:577	arg1	Acid					569:572	Acid Blue 158	569:581	Acid Blue 158 (AB158)	569:589	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	1	3	theme	serious	243:249	arg1	discharge					183:191	The discharge	179:191	The discharge of organic dyes into the aquatic environment	179:236	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	1	3	theme	serious	243:249	arg1	problem					251:257	a serious problem	241:257	a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants	241:350	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	6	4	theme	adsorption	972:981	arg1	data					983:986	The adsorption data	968:986	The adsorption data	968:986	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	4	5	theme	adsorption	807:816	arg1	efficiency					818:827	The adsorption efficiency	803:827	The adsorption efficiency of IC, AB158 and RB4 dye molecules	803:862	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	4	5	theme	adsorption	807:816	arg1	%					874:874	80.41%	869:874	80.41%	869:874	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	2	6	theme	dyes	617:620	arg1	removal					537:543	the removal	533:543	the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water	533:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	0	7	theme	dyes	100:103	arg1	removal					105:111	toxic dyes removal	94:111	toxic dyes removal	94:111	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	1	8	theme	pollutants	341:350	arg1	risks					303:307	possible health risks	287:307	possible health risks	287:307	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	1	8	theme	pollutants	341:350	arg1	hazards					324:330	ecological hazards	313:330	ecological hazards of these pollutants	313:350	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	4	9	theme	IC	832:833	arg1	molecules					854:862	IC, AB158 and RB4 dye molecules	832:862	IC, AB158 and RB4 dye molecules	832:862	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	10	10	from	efficiency	1480:1489	arg1	process					1537:1543	water/wastewater treatment process	1510:1543	water/wastewater treatment process	1510:1543	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	0	11	theme	toxic	94:98	arg1	removal					105:111	toxic dyes removal	94:111	toxic dyes removal	94:111	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	9	12	from	nature	1414:1419	arg1	spontaneous					1383:1393	spontaneous	1383:1393	spontaneous	1383:1393	The thermodynamic studies revealed that the adsorption was spontaneous and endothermic in nature.					
32553962	9	12	from	nature	1414:1419	arg1	adsorption					1368:1377	the adsorption	1364:1377	the adsorption	1364:1377	The thermodynamic studies revealed that the adsorption was spontaneous and endothermic in nature.					
32553962	2	13	theme	RB4	612:614	arg1	dyes					617:620	Reactive Blue 4 (RB4) dyes	595:620	Reactive Blue 4 (RB4) dyes from water	595:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	10	14	theme	LCB	1426:1428	arg1	composite					1430:1438	The LCB composite	1422:1438	The LCB composite	1422:1438	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	8	15	theme	adsorbent	1220:1228	arg1	material					1230:1237	The fabricated adsorbent material	1205:1237	The fabricated adsorbent material	1205:1237	The fabricated adsorbent material was characterized using PXRD, FTIR, SEM-EDAX, TGA-DSC and surface area measurements.					
32553962	10	16	theme	treatment	1527:1535	arg1	process					1537:1543	water/wastewater treatment process	1510:1543	water/wastewater treatment process	1510:1543	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	8	17	theme	fabricated	1209:1218	arg1	material					1230:1237	The fabricated adsorbent material	1205:1237	The fabricated adsorbent material	1205:1237	The fabricated adsorbent material was characterized using PXRD, FTIR, SEM-EDAX, TGA-DSC and surface area measurements.					
32553962	7	18	used	used	1170:1173	arg2	models					1158:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models were used to describe saturation point.					
32553962	6	19	theme	LCB	1103:1105	arg1	matrix					1107:1112	LCB matrix	1103:1112	LCB matrix	1103:1112	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	7	20	theme	Langmuir	1119:1126	arg1	models					1158:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models were used to describe saturation point.					
32553962	3	21	theme	optimal	669:675	arg1	conditions					677:686	The optimal conditions	665:686	The optimal conditions for the dye adsorption	665:709	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	3	21	theme	optimal	669:675	arg1	pH = 3					728:733	pH = 3	728:733	pH = 3	728:733	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	2	22	theme	Blue	604:607	arg1	dyes					617:620	Reactive Blue 4 (RB4) dyes	595:620	Reactive Blue 4 (RB4) dyes from water	595:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	23	theme	batch	636:640	arg1	techniques					653:662	batch adsorption techniques	636:662	batch adsorption techniques	636:662	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	9	24	from	spontaneous	1383:1393	arg1	nature					1414:1419	nature	1414:1419	nature	1414:1419	The thermodynamic studies revealed that the adsorption was spontaneous and endothermic in nature.					
32553962	2	25	theme	Reactive	595:602	arg1	dyes					617:620	Reactive Blue 4 (RB4) dyes	595:620	Reactive Blue 4 (RB4) dyes from water	595:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	7	26	theme	D-R	1145:1147	arg1	models					1158:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models were used to describe saturation point.					
32553962	6	27	theme	dyes	1095:1098	arg1	adsorption					1077:1086	the physico-chemical adsorption	1056:1086	the physico-chemical adsorption of the dyes on LCB matrix	1056:1112	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	10	28	theme	adsorption-desorption	1458:1478	arg1	efficiency					1480:1489	remarkable adsorption-desorption efficiency	1447:1489	remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process	1447:1543	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	2	29	theme	facile	374:379	arg1	method					381:386	a facile method	372:386	a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques	372:662	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	30	theme	Carmine	555:561	arg1	removal					537:543	the removal	533:543	the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water	533:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	4	31	theme	molecules	854:862	arg1	efficiency					818:827	The adsorption efficiency	803:827	The adsorption efficiency of IC, AB158 and RB4 dye molecules	803:862	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	4	31	theme	molecules	854:862	arg1	%					874:874	80.41%	869:874	80.41%	869:874	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	8	32	theme	surface	1297:1303	arg1	measurements					1310:1321	surface area measurements	1297:1321	surface area measurements	1297:1321	The fabricated adsorbent material was characterized using PXRD, FTIR, SEM-EDAX, TGA-DSC and surface area measurements.					
32553962	6	33	from	adsorption	1077:1086	arg1	matrix					1107:1112	LCB matrix	1103:1112	LCB matrix	1103:1112	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	1	34	theme	organic	196:202	arg1	dyes					204:207	organic dyes	196:207	organic dyes	196:207	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	2	35	theme	material	421:428	arg1	preparation					396:406	the preparation	392:406	the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques	392:662	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	3	36	theme	dye	696:698	arg1	adsorption					700:709	the dye adsorption	692:709	the dye adsorption	692:709	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	4	37	theme	dye	850:852	arg1	molecules					854:862	IC, AB158 and RB4 dye molecules	832:862	IC, AB158 and RB4 dye molecules	832:862	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	0	38	dep	supported	42:50	arg1	Evaluation					114:123	Evaluation	114:123	Evaluation of adsorption kinetics, isotherms and thermodynamics	114:176	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	1	39	theme	dyes	204:207	arg1	discharge					183:191	The discharge	179:191	The discharge of organic dyes into the aquatic environment	179:236	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	1	39	theme	dyes	204:207	arg1	problem					251:257	a serious problem	241:257	a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants	241:350	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	1	40	theme	possible	287:294	arg1	risks					303:307	possible health risks	287:307	possible health risks	287:307	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	0	41	theme	kinetics	139:146	arg1	Evaluation					114:123	Evaluation	114:123	Evaluation of adsorption kinetics, isotherms and thermodynamics	114:176	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	0	42	theme	La	34:35	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	0	42	theme	La	34:35	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	1	43	theme	health	296:301	arg1	risks					303:307	possible health risks	287:307	possible health risks	287:307	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	0	44	theme	adsorption	128:137	arg1	kinetics					139:146	adsorption kinetics	128:146	adsorption kinetics	128:146	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	3	45	dep	concentration	748:760	arg1	50 mg/L					763:769	50 mg/L	763:769	50 mg/L	763:769	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	3	45	dep	concentration	748:760	arg1	dosage					785:790	adsorbent dosage	775:790	adsorbent dosage of 100 mg	775:800	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	8	46	theme	area	1305:1308	arg1	measurements					1310:1321	surface area measurements	1297:1321	surface area measurements	1297:1321	The fabricated adsorbent material was characterized using PXRD, FTIR, SEM-EDAX, TGA-DSC and surface area measurements.					
32553962	9	47	theme	thermodynamic	1328:1340	arg1	studies					1342:1348	The thermodynamic studies	1324:1348	The thermodynamic studies	1324:1348	The thermodynamic studies revealed that the adsorption was spontaneous and endothermic in nature.					
32553962	10	48	theme	water/wastewater	1510:1525	arg1	process					1537:1543	water/wastewater treatment process	1510:1543	water/wastewater treatment process	1510:1543	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	10	49	theme	remarkable	1447:1456	arg1	efficiency					1480:1489	remarkable adsorption-desorption efficiency	1447:1489	remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process	1447:1543	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	1	50	theme	risks	303:307	arg1	persistence					272:282	the persistence	268:282	the persistence of possible health risks and ecological hazards of these pollutants	268:350	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	0	51	theme	isotherms	149:157	arg1	Evaluation					114:123	Evaluation	114:123	Evaluation of adsorption kinetics, isotherms and thermodynamics	114:176	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	4	52	theme	RB4	846:848	arg1	molecules					854:862	IC, AB158 and RB4 dye molecules	832:862	IC, AB158 and RB4 dye molecules	832:862	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	6	53	theme	physico-chemical	1060:1075	arg1	adsorption					1077:1086	the physico-chemical adsorption	1056:1086	the physico-chemical adsorption of the dyes on LCB matrix	1056:1112	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	2	54	theme	Indigo	548:553	arg1	IC					564:565	IC	564:565	IC	564:565	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	54	theme	Indigo	548:553	arg1	Carmine					555:561	Indigo Carmine	548:561	Indigo Carmine (IC)	548:566	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	4	55	theme	AB158	836:840	arg1	molecules					854:862	IC, AB158 and RB4 dye molecules	832:862	IC, AB158 and RB4 dye molecules	832:862	The adsorption efficiency of IC, AB158 and RB4 dye molecules were 80.41%, 83.54% and 86.91% respectively.					
32553962	2	56	theme	Acid	569:572	arg1	removal					537:543	the removal	533:543	the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water	533:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	7	57	theme	Freundlich	1129:1138	arg1	models					1158:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models were used to describe saturation point.					
32553962	1	58	theme	ecological	313:322	arg1	hazards					324:330	ecological hazards	313:330	ecological hazards of these pollutants	313:350	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	0	59	theme	thermodynamics	163:176	arg1	Evaluation					114:123	Evaluation	114:123	Evaluation of adsorption kinetics, isotherms and thermodynamics	114:176	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	2	60	theme	adsorption	642:651	arg1	techniques					653:662	batch adsorption techniques	636:662	batch adsorption techniques	636:662	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	10	61	theme	dye	1616:1618	arg1	removal					1620:1626	dye removal	1616:1626	dye removal	1616:1626	The LCB composite showed remarkable adsorption-desorption efficiency for dye removal in water/wastewater treatment process; hence it can be considered as a competent and potential adsorbent for dye removal.					
32553962	1	62	theme	aquatic	218:224	arg1	environment					226:236	the aquatic environment	214:236	the aquatic environment	214:236	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	3	63	theme	adsorbent	775:783	arg1	dosage					785:790	adsorbent dosage	775:790	adsorbent dosage of 100 mg	775:800	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	7	64	theme	saturation	1187:1196	arg1	point					1198:1202	saturation point	1187:1202	saturation point	1187:1202	The Langmuir, Freundlich, and D-R isotherm models were used to describe saturation point.					
32553962	0	65	theme	carboxymethylcellulose-clay	52:78	arg1	composite					80:88	carboxymethylcellulose-clay composite	52:88	carboxymethylcellulose-clay composite for toxic dyes removal	52:111	Synthesis and characterization of La(III) supported carboxymethylcellulose-clay composite for toxic dyes removal: Evaluation of adsorption kinetics, isotherms and thermodynamics.					
32553962	2	66	from	removal	537:543	arg1	water					627:631	water	627:631	water	627:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	5	67	theme	entire	913:918	arg1	process					931:937	The entire adsorption process	909:937	The entire adsorption process	909:937	The entire adsorption process was completed within 40 min.					
32553962	2	68	theme	composite	411:419	arg1	material					421:428	composite material	411:428	composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques	411:662	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	69	from	water	627:631	arg1	AB158					584:588	AB158	584:588	AB158	584:588	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	69	from	water	627:631	arg1	removal					537:543	the removal	533:543	the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water	533:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	69	from	water	627:631	arg1	dyes					617:620	Reactive Blue 4 (RB4) dyes	595:620	Reactive Blue 4 (RB4) dyes from water	595:631	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	69	from	water	627:631	arg1	Acid					569:572	Acid Blue 158	569:581	Acid Blue 158 (AB158)	569:589	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	69	from	water	627:631	arg1	Carmine					555:561	Indigo Carmine	548:561	Indigo Carmine (IC)	548:566	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	2	69	from	water	627:631	arg1	IC					564:565	IC	564:565	IC	564:565	This paper reports a facile method for the preparation of composite material consisting of lanthanum incorporated carboxymethylcellulose-bentonite (LCB) composite and utilized for the removal of Indigo Carmine (IC), Acid Blue 158 (AB158) and Reactive Blue 4 (RB4) dyes from water by batch adsorption techniques.					
32553962	3	70	theme	100 mg	795:800	arg1	50 mg/L					763:769	50 mg/L	763:769	50 mg/L	763:769	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	3	70	theme	100 mg	795:800	arg1	dosage					785:790	adsorbent dosage	775:790	adsorbent dosage of 100 mg	775:800	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	3	71	theme	initial	736:742	arg1	concentration					748:760	initial dye concentration	736:760	initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg	736:800	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	3	71	theme	initial	736:742	arg1	pH = 3					728:733	pH = 3	728:733	pH = 3	728:733	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	7	72	theme	isotherm	1149:1156	arg1	models					1158:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models	1115:1163	The Langmuir, Freundlich, and D-R isotherm models were used to describe saturation point.					
32553962	1	73	theme	hazards	324:330	arg1	persistence					272:282	the persistence	268:282	the persistence of possible health risks and ecological hazards of these pollutants	268:350	The discharge of organic dyes into the aquatic environment is a serious problem owing to the persistence of possible health risks and ecological hazards of these pollutants.					
32553962	5	74	theme	adsorption	920:929	arg1	process					931:937	The entire adsorption process	909:937	The entire adsorption process	909:937	The entire adsorption process was completed within 40 min.					
32553962	6	75	theme	kinetic	1027:1033	arg1	model					1035:1039	the pseudo-second-order kinetic model	1003:1039	the pseudo-second-order kinetic model	1003:1039	The adsorption data fits well with the pseudo-second-order kinetic model, demonstrating the physico-chemical adsorption of the dyes on LCB matrix.					
32553962	3	76	theme	dye	744:746	arg1	concentration					748:760	initial dye concentration	736:760	initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg	736:800	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
32553962	3	76	theme	dye	744:746	arg1	pH = 3					728:733	pH = 3	728:733	pH = 3	728:733	The optimal conditions for the dye adsorption were found to be pH = 3, initial dye concentration: 50 mg/L and adsorbent dosage of 100 mg.					
34963503	6	0	theme	significant	987:997	arg1	differences					999:1009	No significant differences	984:1009	No significant differences	984:1009	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	3	1	theme	glucose	475:481	arg1	tolerance					483:491	glucose tolerance	475:491	glucose tolerance	475:491	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	5	2	theme	glucose	910:916	arg1	tolerance					918:926	oral glucose tolerance	905:926	oral glucose tolerance	905:926	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	7	3	theme	relative	1149:1156	arg1	suggestive					1271:1280	suggestive	1271:1280	suggestive	1271:1280	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	3	theme	relative	1149:1156	arg1	abundance					1158:1166	A decreased relative abundance	1137:1166	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05)	1137:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	3	4	from	tolerance	483:491	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	3	5	theme	young	518:522	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	8	6	theme	reared	1395:1400	arg1	rats					1411:1414	artificially reared suckling rats	1382:1414	artificially reared suckling rats	1382:1414	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	2	7	theme	formula	274:280	arg1	oligosaccharides					193:208	Human milk oligosaccharides	182:208	Human milk oligosaccharides (HMO)	182:214	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	7	theme	formula	274:280	arg1	components					224:233	key components	220:233	key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota	220:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	8	8	theme	rats	1411:1414	arg1	microbiota					1368:1377	the faecal microbiota	1357:1377	the faecal microbiota of artificially reared suckling rats	1357:1414	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	8	9	theme	=	1541:1541	arg1	P					1539:1539	P	1539:1539	P = 0·479	1539:1547	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	7	10	theme	SL	1329:1330	arg1	effect					1317:1322	a potential pathogen inhibitory effect	1285:1322	a potential pathogen inhibitory effect of 3'SL	1285:1330	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	9	11	theme	protective	1573:1582	arg1	effects					1584:1590	the known protective effects	1563:1590	the known protective effects of HMO	1563:1597	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	4	12	dep	FL	745:746	arg1	CTR					756:758	CTR	756:758	CTR	756:758	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	12	dep	FL	745:746	arg1	2'-FL					770:774	0·6 g/l 2'-FL	762:774	0·6 g/l 2'-FL	762:774	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	2	13	theme	breast	238:243	arg1	milk					245:248	breast milk	238:248	breast milk but not standard infant formula	238:280	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	7	14	theme	effect	1317:1322	arg1	suggestive					1271:1280	suggestive	1271:1280	suggestive	1271:1280	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	14	theme	effect	1317:1322	arg1	abundance					1158:1166	A decreased relative abundance	1137:1166	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05)	1137:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	4	15	theme	g/l	713:715	arg1	3'-sialyllactose					717:732	1·2 g/l 3'-sialyllactose	709:732	1·2 g/l 3'-sialyllactose	709:732	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	8	16	theme	=	1450:1450	arg1	P					1448:1448	P = 0·001	1448:1456	P = 0·001	1448:1456	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	8	16	theme	=	1450:1450	arg1	age					1443:1445	age	1443:1445	age (P = 0·001)	1443:1457	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	7	17	theme	pathogen	1297:1304	arg1	effect					1317:1322	a potential pathogen inhibitory effect	1285:1322	a potential pathogen inhibitory effect of 3'SL	1285:1330	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	4	18	dep	milk	631:634	arg1	substitute					636:645	substitute	636:645	substitute	636:645	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	2	19	theme	standard	258:265	arg1	formula					274:280	standard infant formula	258:280	breast milk but not standard infant formula	238:280	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	1	20	theme	neonatal	149:156	arg1	development					158:168	neonatal development	149:168	neonatal development	149:168	Early life nutrition fundamentally influences neonatal development and health.					
34963503	9	21	theme	supplementation	1625:1639	arg1	investigation					1608:1620	further investigation	1600:1620	further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition	1600:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	5	22	theme	food	832:835	arg1	intake					837:842	food intake	832:842	food intake	832:842	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	3	23	from	microbiota	504:513	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	0	24	theme	reared	82:87	arg1	rats					97:100	artificially reared newborn rats	69:100	artificially reared newborn rats	69:100	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	3	25	theme	rearing	364:370	arg1	system					372:377	an artificial rearing system	350:377	an artificial rearing system	350:377	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	0	26	from	Effect	0:5	arg1	rats					97:100	artificially reared newborn rats	69:100	artificially reared newborn rats	69:100	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	9	27	theme	other	1656:1660	arg1	HMO					1662:1664	other HMO	1656:1664	other HMO	1656:1664	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	4	28	dep	CTR	622:624	arg1	1					619:619	1	619:619	1	619:619	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	28	dep	CTR	622:624	arg1	FL					655:656	FL	655:656	(1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose)	618:733	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	28	dep	CTR	622:624	arg1	2'-fucosyllactose					673:689	1·2 g/l 2'-fucosyllactose	665:689	1·2 g/l 2'-fucosyllactose	665:689	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	28	dep	CTR	622:624	arg1	3'-sialyllactose					717:732	1·2 g/l 3'-sialyllactose	709:732	1·2 g/l 3'-sialyllactose	709:732	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	28	dep	CTR	622:624	arg1	SL					699:700	SL	699:700	(1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose)	618:733	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	28	dep	CTR	622:624	arg1	CTR					659:661	CTR	659:661	CTR	659:661	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	28	dep	CTR	622:624	arg1	CTR					703:705	CTR	703:705	CTR	703:705	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	7	29	theme	<	1259:1259	arg1	0·05					1261:1264	P < 0·05	1257:1264	P < 0·05	1257:1264	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	29	theme	<	1259:1259	arg1	samples					1248:1254	Terrisporobacter in week 3 faecal samples	1214:1254	Terrisporobacter in week 3 faecal samples (P < 0·05)	1214:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	3	30	theme	HMO	424:426	arg1	effect					410:415	the effect	406:415	the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups	406:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	6	31	theme	body	1063:1066	arg1	composition					1068:1078	body composition	1063:1078	body composition	1063:1078	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	8	32	theme	2'-FL	1495:1499	arg1	presence					1483:1490	the presence	1479:1490	the presence of 2'-FL and/or 3'-SL	1479:1512	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	4	33	dep	21	559:560	arg1	to					556:557	to	556:557	to	556:557	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	8	34	theme	3'-SL	1508:1512	arg1	presence					1483:1490	the presence	1479:1490	the presence of 2'-FL and/or 3'-SL	1479:1512	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	9	35	from	supplementation	1625:1639	arg1	models					1669:1674	models	1669:1674	models of premature birth, extremely low BW or malnutrition	1669:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	4	36	theme	g/l	782:784	arg1	3'-SL					786:790	0·6 g/l 3'-SL	778:790	0·6 g/l 3'-SL	778:790	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	9	37	theme	birth	1689:1693	arg1	models					1669:1674	models	1669:1674	models of premature birth, extremely low BW or malnutrition	1669:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	0	38	theme	supplementation	10:24	arg1	Effect					0:5	Effect	0:5	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.	0:101	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	1	39	theme	life	109:112	arg1	nutrition					114:122	Early life nutrition	103:122	Early life nutrition	103:122	Early life nutrition fundamentally influences neonatal development and health.					
34963503	9	40	from	investigation	1608:1620	arg1	models					1669:1674	models	1669:1674	models of premature birth, extremely low BW or malnutrition	1669:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	6	41	theme	microbes	1127:1134	arg1	abundance					1098:1106	abundance	1098:1106	abundance	1098:1106	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	6	41	theme	microbes	1127:1134	arg1	performance					1050:1060	growth performance	1043:1060	growth performance	1043:1060	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	6	41	theme	microbes	1127:1134	arg1	composition					1068:1078	body composition	1063:1078	body composition	1063:1078	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	6	41	theme	microbes	1127:1134	arg1	weight					1087:1092	organ weight	1081:1092	organ weight	1081:1092	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	0	42	theme	select	31:36	arg1	oligosaccharides					49:64	select human milk oligosaccharides	31:64	select human milk oligosaccharides	31:64	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	4	43	theme	g/l	766:768	arg1	2'-FL					770:774	0·6 g/l 2'-FL	762:774	0·6 g/l 2'-FL	762:774	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	2	44	theme	Human	182:186	arg1	oligosaccharides					193:208	Human milk oligosaccharides	182:208	Human milk oligosaccharides (HMO)	182:214	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	44	theme	Human	182:186	arg1	components					224:233	key components	220:233	key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota	220:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	44	theme	Human	182:186	arg1	HMO					211:213	HMO	211:213	HMO	211:213	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	0	45	with	supplementation	10:24	arg1	oligosaccharides					49:64	select human milk oligosaccharides	31:64	select human milk oligosaccharides	31:64	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	0	46	theme	milk	44:47	arg1	oligosaccharides					49:64	select human milk oligosaccharides	31:64	select human milk oligosaccharides	31:64	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	6	47	from	groups	1033:1038	arg1	performance					1050:1060	growth performance	1043:1060	growth performance	1043:1060	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	6	47	from	groups	1033:1038	arg1	abundance					1098:1106	abundance	1098:1106	abundance	1098:1106	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	6	47	from	groups	1033:1038	arg1	composition					1068:1078	body composition	1063:1078	body composition	1063:1078	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	6	47	from	groups	1033:1038	arg1	weight					1087:1092	organ weight	1081:1092	organ weight	1081:1092	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	9	48	theme	low	1706:1708	arg1	BW					1710:1711	extremely low BW	1696:1711	extremely low BW	1696:1711	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	9	49	theme	malnutrition	1716:1727	arg1	models					1669:1674	models	1669:1674	models of premature birth, extremely low BW or malnutrition	1669:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	2	50	theme	microbiota	332:341	arg1	establishment					299:311	the establishment	295:311	the establishment of the newborn gut microbiota	295:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	5	51	theme	body	929:932	arg1	composition					934:944	body composition	929:944	body composition	929:944	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	4	52	theme	g/l	669:671	arg1	2'-fucosyllactose					673:689	1·2 g/l 2'-fucosyllactose	665:689	1·2 g/l 2'-fucosyllactose	665:689	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	7	53	theme	faecal	1195:1200	arg1	samples					1202:1208	week 1 faecal samples	1188:1208	week 1 faecal samples	1188:1208	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	4	54	theme	Sprague-Dawley	563:576	arg1	rats					578:581	Sprague-Dawley rats	563:581	Sprague-Dawley rats	563:581	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	9	55	theme	pronounced	1743:1752	arg1	outcomes					1754:1761	more pronounced outcomes	1738:1761	more pronounced outcomes	1738:1761	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	8	56	theme	rat	1517:1519	arg1	milk					1521:1524	rat milk substitutes	1517:1536	rat milk substitutes (P = 0·479)	1517:1548	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	5	57	theme	oral	905:908	arg1	tolerance					918:926	oral glucose tolerance	905:926	oral glucose tolerance	905:926	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	7	58	theme	week	1188:1191	arg1	samples					1202:1208	week 1 faecal samples	1188:1208	week 1 faecal samples	1188:1208	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	9	59	theme	BW	1710:1711	arg1	models					1669:1674	models	1669:1674	models of premature birth, extremely low BW or malnutrition	1669:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	4	60	theme	postnatal	539:547	arg1	days					549:552	postnatal days 4 to 21	539:560	postnatal days 4 to 21	539:560	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	7	61	theme	Proteus	1177:1183	arg1	suggestive					1271:1280	suggestive	1271:1280	suggestive	1271:1280	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	61	theme	Proteus	1177:1183	arg1	abundance					1158:1166	A decreased relative abundance	1137:1166	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05)	1137:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	3	62	theme	faecal	497:502	arg1	microbiota					504:513	faecal microbiota	497:513	faecal microbiota	497:513	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	6	63	theme	dominant	1111:1118	arg1	microbes					1127:1134	dominant faecal microbes	1111:1134	dominant faecal microbes	1111:1134	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	7	64	theme	decreased	1139:1147	arg1	suggestive					1271:1280	suggestive	1271:1280	suggestive	1271:1280	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	64	theme	decreased	1139:1147	arg1	abundance					1158:1166	A decreased relative abundance	1137:1166	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05)	1137:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	5	65	theme	faecal	866:871	arg1	samples					873:879	faecal samples	866:879	faecal samples	866:879	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	3	66	from	growth	456:461	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	2	67	theme	newborn	320:326	arg1	microbiota					332:341	the newborn gut microbiota	316:341	the newborn gut microbiota	316:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	8	68	theme	suckling	1402:1409	arg1	rats					1411:1414	artificially reared suckling rats	1382:1414	artificially reared suckling rats	1382:1414	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	9	69	theme	known	1567:1571	arg1	effects					1584:1590	the known protective effects	1563:1590	the known protective effects of HMO	1563:1597	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	3	70	from	adiposity	464:472	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	3	71	theme	rat	524:526	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	6	72	theme	growth	1043:1048	arg1	performance					1050:1060	growth performance	1043:1060	growth performance	1043:1060	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	2	73	theme	milk	245:248	arg1	oligosaccharides					193:208	Human milk oligosaccharides	182:208	Human milk oligosaccharides (HMO)	182:214	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	73	theme	milk	245:248	arg1	components					224:233	key components	220:233	key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota	220:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	7	74	theme	inhibitory	1306:1315	arg1	effect					1317:1322	a potential pathogen inhibitory effect	1285:1322	a potential pathogen inhibitory effect of 3'SL	1285:1330	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	9	75	theme	HMO	1595:1597	arg1	effects					1584:1590	the known protective effects	1563:1590	the known protective effects of HMO	1563:1597	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	7	76	theme	potential	1287:1295	arg1	effect					1317:1322	a potential pathogen inhibitory effect	1285:1322	a potential pathogen inhibitory effect of 3'SL	1285:1330	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	4	77	theme	1·2	709:711	arg1	3'-sialyllactose					717:732	1·2 g/l 3'-sialyllactose	709:732	1·2 g/l 3'-sialyllactose	709:732	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	9	78	theme	further	1600:1606	arg1	investigation					1608:1620	further investigation	1600:1620	further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition	1600:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	4	79	theme	rat	627:629	arg1	CTR					622:624	(1) CTR	618:624	(1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose)	618:733	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	79	theme	rat	627:629	arg1	milk					631:634	rat milk substitute	627:645	rat milk substitute	627:645	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	2	80	theme	infant	267:272	arg1	formula					274:280	standard infant formula	258:280	breast milk but not standard infant formula	238:280	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	3	81	theme	organ	450:454	arg1	growth					456:461	organ growth	450:461	organ growth	450:461	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	5	82	theme	Body	794:797	arg1	BW					807:808	BW	807:808	BW	807:808	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	5	82	theme	Body	794:797	arg1	weight					799:804	Body weight	794:804	Body weight (BW)	794:809	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	8	83	dep	=	1541:1541	arg1	0·479					1543:1547	0·479	1543:1547	0·479	1543:1547	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	4	84	theme	FL	745:746	arg1	CTR					622:624	(1) CTR	618:624	(1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose)	618:733	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	84	theme	FL	745:746	arg1	FL					745:746	(4) 2'FL	739:746	(4) 2'FL	739:746	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	84	theme	FL	745:746	arg1	one					610:612	one	610:612	one	610:612	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	84	theme	FL	745:746	arg1	milk					631:634	rat milk substitute	627:645	rat milk substitute	627:645	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	9	85	with	supplementation	1625:1639	arg1	HMO					1662:1664	other HMO	1656:1664	other HMO	1656:1664	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	9	85	with	supplementation	1625:1639	arg1	these					1646:1650	these	1646:1650	these	1646:1650	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	0	86	theme	newborn	89:95	arg1	rats					97:100	artificially reared newborn rats	69:100	artificially reared newborn rats	69:100	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	4	87	theme	CTR	622:624	arg1	CTR					622:624	(1) CTR	618:624	(1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose)	618:733	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	87	theme	CTR	622:624	arg1	FL					745:746	(4) 2'FL	739:746	(4) 2'FL	739:746	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	87	theme	CTR	622:624	arg1	one					610:612	one	610:612	one	610:612	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	4	87	theme	CTR	622:624	arg1	milk					631:634	rat milk substitute	627:645	rat milk substitute	627:645	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	6	88	theme	organ	1081:1085	arg1	weight					1087:1092	organ weight	1081:1092	organ weight	1081:1092	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	3	89	from	effect	410:415	arg1	body					441:444	the whole body	431:444	the whole body	431:444	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	3	89	from	effect	410:415	arg1	growth					456:461	organ growth	450:461	organ growth	450:461	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	7	90	from	abundance	1158:1166	arg1	0·05					1261:1264	P < 0·05	1257:1264	P < 0·05	1257:1264	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	90	from	abundance	1158:1166	arg1	samples					1248:1254	Terrisporobacter in week 3 faecal samples	1214:1254	Terrisporobacter in week 3 faecal samples (P < 0·05)	1214:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	90	from	abundance	1158:1166	arg1	samples					1202:1208	week 1 faecal samples	1188:1208	week 1 faecal samples	1188:1208	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	91	theme	P	1257:1257	arg1	0·05					1261:1264	P < 0·05	1257:1264	P < 0·05	1257:1264	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	91	theme	P	1257:1257	arg1	samples					1248:1254	Terrisporobacter in week 3 faecal samples	1214:1254	Terrisporobacter in week 3 faecal samples (P < 0·05)	1214:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	8	92	dep	milk	1521:1524	arg1	P					1539:1539	P	1539:1539	P = 0·479	1539:1547	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	8	92	dep	milk	1521:1524	arg1	substitutes					1526:1536	substitutes	1526:1536	substitutes	1526:1536	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	8	93	from	changes	1346:1352	arg1	microbiota					1368:1377	the faecal microbiota	1357:1377	the faecal microbiota of artificially reared suckling rats	1357:1414	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	5	94	theme	bowel	812:816	arg1	movements					818:826	bowel movements	812:826	bowel movements	812:826	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
34963503	3	95	theme	artificial	353:362	arg1	rearing					364:370	an artificial rearing	350:370	an artificial rearing system	350:377	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	9	96	theme	premature	1679:1687	arg1	birth					1689:1693	premature birth	1679:1693	premature birth	1679:1693	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	1	97	theme	Early	103:107	arg1	nutrition					114:122	Early life nutrition	103:122	Early life nutrition	103:122	Early life nutrition fundamentally influences neonatal development and health.					
34963503	4	98	theme	0·6	778:780	arg1	3'-SL					786:790	0·6 g/l 3'-SL	778:790	0·6 g/l 3'-SL	778:790	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	0	99	theme	human	38:42	arg1	oligosaccharides					49:64	select human milk oligosaccharides	31:64	select human milk oligosaccharides	31:64	Effect of supplementation with select human milk oligosaccharides on artificially reared newborn rats.					
34963503	2	100	theme	milk	188:191	arg1	oligosaccharides					193:208	Human milk oligosaccharides	182:208	Human milk oligosaccharides (HMO)	182:214	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	100	theme	milk	188:191	arg1	components					224:233	key components	220:233	key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota	220:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	100	theme	milk	188:191	arg1	HMO					211:213	HMO	211:213	HMO	211:213	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	3	101	theme	whole	435:439	arg1	body					441:444	the whole body	431:444	the whole body	431:444	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	6	102	theme	faecal	1120:1125	arg1	microbes					1127:1134	dominant faecal microbes	1111:1134	dominant faecal microbes	1111:1134	No significant differences were observed between groups in growth performance, body composition, organ weight and abundance of dominant faecal microbes.					
34963503	7	103	theme	faecal	1241:1246	arg1	0·05					1261:1264	P < 0·05	1257:1264	P < 0·05	1257:1264	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	103	theme	faecal	1241:1246	arg1	samples					1248:1254	Terrisporobacter in week 3 faecal samples	1214:1254	Terrisporobacter in week 3 faecal samples (P < 0·05)	1214:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	4	104	theme	0·6	762:764	arg1	2'-FL					770:774	0·6 g/l 2'-FL	762:774	0·6 g/l 2'-FL	762:774	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	2	105	theme	key	220:222	arg1	oligosaccharides					193:208	Human milk oligosaccharides	182:208	Human milk oligosaccharides (HMO)	182:214	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	2	105	theme	key	220:222	arg1	components					224:233	key components	220:233	key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota	220:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	3	106	from	body	441:444	arg1	pups					528:531	young rat pups	518:531	young rat pups	518:531	Using an artificial rearing system, our objective was to test the effect of two HMO on the whole body and organ growth, adiposity, glucose tolerance and faecal microbiota in young rat pups.					
34963503	8	107	theme	Longitudinal	1333:1344	arg1	changes					1346:1352	Longitudinal changes	1333:1352	Longitudinal changes in the faecal microbiota of artificially reared suckling rats	1333:1414	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	9	108	from	models	1669:1674	arg1	investigation					1608:1620	further investigation	1600:1620	further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition	1600:1727	Considering the known protective effects of HMO, further investigation of supplementation with these and other HMO in models of premature birth, extremely low BW or malnutrition may show more pronounced outcomes.					
34963503	2	109	theme	gut	328:330	arg1	microbiota					332:341	the newborn gut microbiota	316:341	the newborn gut microbiota	316:341	Human milk oligosaccharides (HMO) are key components of breast milk but not standard infant formula that support the establishment of the newborn gut microbiota.					
34963503	7	110	theme	Terrisporobacter	1214:1229	arg1	0·05					1261:1264	P < 0·05	1257:1264	P < 0·05	1257:1264	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	7	110	theme	Terrisporobacter	1214:1229	arg1	samples					1248:1254	Terrisporobacter in week 3 faecal samples	1214:1254	Terrisporobacter in week 3 faecal samples (P < 0·05)	1214:1265	A decreased relative abundance of genus Proteus in week 1 faecal samples and Terrisporobacter in week 3 faecal samples (P < 0·05) was suggestive of a potential pathogen inhibitory effect of 3'SL.					
34963503	4	111	theme	1·2	665:667	arg1	2'-fucosyllactose					673:689	1·2 g/l 2'-fucosyllactose	665:689	1·2 g/l 2'-fucosyllactose	665:689	From postnatal days 4 to 21, Sprague-Dawley rats were randomised to receive one of: (1) CTR (rat milk substitute); (2) 2'FL (CTR + 1·2 g/l 2'-fucosyllactose); (3) 3'SL (CTR + 1·2 g/l 3'-sialyllactose) and (4) 2'FL + 3'SL (CTR + 0·6 g/l 2'-FL + 0·6 g/l 3'-SL).					
34963503	8	112	theme	faecal	1361:1366	arg1	microbiota					1368:1377	the faecal microbiota	1357:1377	the faecal microbiota of artificially reared suckling rats	1357:1414	Longitudinal changes in the faecal microbiota of artificially reared suckling rats were primarily governed by age (P = 0·001) and not affected by the presence of 2'-FL and/or 3'-SL in rat milk substitutes (P = 0·479).					
34963503	5	113	theme	organ	950:954	arg1	weight					956:961	organ weight	950:961	organ weight	950:961	Body weight (BW), bowel movements and food intake were monitored daily, faecal samples collected each week and oral glucose tolerance, body composition and organ weight measured at weaning.					
33009829	7	0	theme	COVID-19	1530:1537	arg1	severity					1539:1546	COVID-19 severity	1530:1546	COVID-19 severity	1530:1546	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	8	1	theme	disease	1682:1688	arg1	symptoms					1703:1710	severe disease inflammatory symptoms	1675:1710	severe disease inflammatory symptoms	1675:1710	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	3	2	theme	M	713:713	arg1	antibodies					725:734	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies	687:734	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	687:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	1	3	theme	worldwide	237:245	arg1	millions					218:225	affected millions	209:225	affected millions of people worldwide	209:245	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	6	4	theme	COVID-19	1218:1225	arg1	stages					1208:1213	different stages	1198:1213	different stages of COVID-19	1198:1225	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	2	5	theme	immunological	272:284	arg1	mechanisms					286:295	the immunological mechanisms	268:295	the immunological mechanisms involved in disease symptomatology and protective response	268:354	Characterization of the immunological mechanisms involved in disease symptomatology and protective response is important to progress in disease control and prevention.					
33009829	7	6	theme	isotype	1494:1500	arg1	composition					1502:1512	anti-α-Gal antibody isotype composition	1474:1512	anti-α-Gal antibody isotype composition	1474:1512	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	4	7	theme	immune	877:882	arg1	mechanisms					884:893	various immune mechanisms	869:893	various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses	869:996	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	7	8	theme	antibody	1440:1447	arg1	titers					1449:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	3	9	theme	/IgG	720:723	arg1	antibodies					725:734	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies	687:734	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	687:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	1	10	theme	respiratory	157:167	arg1	coronavirus					178:188	severe acute respiratory syndrome coronavirus 2	144:190	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	144:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	1	10	theme	respiratory	157:167	arg1	SARS-CoV-2					193:202	SARS-CoV-2	193:202	SARS-CoV-2	193:202	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	7	11	theme	anti-α-Gal	1474:1483	arg1	composition					1502:1512	anti-α-Gal antibody isotype composition	1474:1512	anti-α-Gal antibody isotype composition	1474:1512	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	3	12	theme	bacterial	760:768	arg1	microbiota					770:779	bacterial microbiota	760:779	bacterial microbiota	760:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	2	13	theme	disease	309:315	arg1	symptomatology					317:330	disease symptomatology	309:330	disease symptomatology	309:330	Characterization of the immunological mechanisms involved in disease symptomatology and protective response is important to progress in disease control and prevention.					
33009829	1	14	contain	has	205:207	arg1	pandemic					125:132	The coronavirus disease 2019 (COVID-19) pandemic	85:132	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	85:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	1	14	contain	has	205:207	arg2	millions					218:225	affected millions	209:225	affected millions of people worldwide	209:245	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	7	15	theme	IgM	1427:1429	arg1	titers					1449:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	7	16	theme	IgE	1422:1424	arg1	titers					1449:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	6	17	theme	control	1258:1264	arg1	individuals					1266:1276	healthy control individuals	1250:1276	healthy control individuals	1250:1276	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	4	18	theme	infectious	947:956	arg1	diseases					958:965	infectious diseases	947:965	infectious diseases without inflammatory responses	947:996	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	3	19	theme	pathogenic	581:590	arg1	viruses					592:598	pathogenic viruses	581:598	pathogenic viruses	581:598	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	9	20	theme	commensal	1804:1812	arg1	bacteria					1814:1821	commensal bacteria	1804:1821	commensal bacteria with α-Gal epitopes	1804:1841	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	0	21	theme	disease	67:73	arg1	symptoms					75:82	COVID-19 disease symptoms	58:82	COVID-19 disease symptoms	58:82	The antibody response to the glycan α-Gal correlates with COVID-19 disease symptoms.					
33009829	1	22	theme	severe	144:149	arg1	coronavirus					178:188	severe acute respiratory syndrome coronavirus 2	144:190	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	144:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	1	22	theme	severe	144:149	arg1	SARS-CoV-2					193:202	SARS-CoV-2	193:202	SARS-CoV-2	193:202	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	6	23	with	comparison	1234:1243	arg1	individuals					1266:1276	healthy control individuals	1250:1276	healthy control individuals	1250:1276	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	7	24	dep	response	1326:1333	arg1	titers					1380:1385	IgG antibody titers	1367:1385	IgG antibody titers	1367:1385	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	7	25	theme	antibody	1371:1378	arg1	titers					1380:1385	IgG antibody titers	1367:1385	IgG antibody titers	1367:1385	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	9	26	theme	protective	1895:1904	arg1	response					1913:1920	α-Gal-induced protective immune response	1881:1920	α-Gal-induced protective immune response	1881:1920	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	1	27	theme	disease	101:107	arg1	pandemic					125:132	The coronavirus disease 2019 (COVID-19) pandemic	85:132	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	85:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	8	28	theme	α-Gal-induced	1598:1610	arg1	response					1619:1626	the α-Gal-induced immune response	1594:1626	the α-Gal-induced immune response	1594:1626	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	4	29	dep	opsonization	835:846	arg1	addition					785:792	addition	785:792	addition	785:792	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	8	30	theme	response	1619:1626	arg1	inhibition					1580:1589	the inhibition	1576:1589	the inhibition of the α-Gal-induced immune response	1576:1626	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	3	31	theme	immunoglobulin	698:711	arg1	IgM					716:718	IgM	716:718	IgM	716:718	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	3	31	theme	immunoglobulin	698:711	arg1	M					713:713	immunoglobulin M	698:713	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	687:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	4	32	theme	antibody-mediated	808:824	arg1	opsonization					835:846	anti-α-Gal antibody-mediated pathogen opsonization	797:846	anti-α-Gal antibody-mediated pathogen opsonization	797:846	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	6	33	from	response	1165:1172	arg1	comparison					1234:1243	comparison	1234:1243	comparison with healthy control individuals	1234:1276	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	6	33	from	response	1165:1172	arg1	stages					1208:1213	different stages	1198:1213	different stages of COVID-19	1198:1225	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	6	33	from	response	1165:1172	arg1	patients					1186:1193	patients	1186:1193	patients at different stages of COVID-19	1186:1225	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	2	34	theme	protective	336:345	arg1	response					347:354	protective response	336:354	protective response	336:354	Characterization of the immunological mechanisms involved in disease symptomatology and protective response is important to progress in disease control and prevention.					
33009829	5	35	theme	immune	1039:1044	arg1	response					1046:1053	the immune response	1035:1053	the immune response to α-Gal	1035:1062	In this study, we hypothesized that the immune response to α-Gal may contribute to the control of COVID-19.					
33009829	2	36	theme	disease	384:390	arg1	control					392:398	progress in disease control	372:398	progress in disease control	372:398	Characterization of the immunological mechanisms involved in disease symptomatology and protective response is important to progress in disease control and prevention.					
33009829	3	37	contain	containing	625:634	arg1	viruses					592:598	pathogenic viruses	581:598	pathogenic viruses	581:598	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	3	37	contain	containing	625:634	arg2	modification					641:652	this modification	636:652	this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	636:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	1	38	theme	people	230:235	arg1	worldwide					237:245	people worldwide	230:245	people worldwide	230:245	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	7	39	theme	composition	1502:1512	arg1	reduction					1398:1406	reduction	1398:1406	reduction in anti-α-Gal IgE, IgM, and IgG antibody titers	1398:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	7	39	theme	composition	1502:1512	arg1	alteration					1460:1469	alteration	1460:1469	alteration of anti-α-Gal antibody isotype composition	1460:1512	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	8	40	theme	severe	1675:1680	arg1	symptoms					1703:1710	severe disease inflammatory symptoms	1675:1710	severe disease inflammatory symptoms	1675:1710	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	3	41	from	modification	641:652	arg1	proteins					666:673	membrane proteins	657:673	membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	657:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	2	42	theme	mechanisms	286:295	arg1	Characterization					248:263	Characterization	248:263	Characterization of the immunological mechanisms involved in disease symptomatology and protective response	248:354	Characterization of the immunological mechanisms involved in disease symptomatology and protective response is important to progress in disease control and prevention.					
33009829	7	43	theme	antibody	1485:1492	arg1	composition					1502:1512	anti-α-Gal antibody isotype composition	1474:1512	anti-α-Gal antibody isotype composition	1474:1512	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	8	44	theme	inflammatory	1690:1701	arg1	symptoms					1703:1710	severe disease inflammatory symptoms	1675:1710	severe disease inflammatory symptoms	1675:1710	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	1	45	theme	acute	151:155	arg1	coronavirus					178:188	severe acute respiratory syndrome coronavirus 2	144:190	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	144:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	1	45	theme	acute	151:155	arg1	SARS-CoV-2					193:202	SARS-CoV-2	193:202	SARS-CoV-2	193:202	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	4	46	theme	various	869:875	arg1	mechanisms					884:893	various immune mechanisms	869:893	various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses	869:996	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	6	47	theme	different	1198:1206	arg1	stages					1208:1213	different stages	1198:1213	different stages of COVID-19	1198:1225	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	3	48	theme	protective	553:562	arg1	response					564:571	a protective response	551:571	a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	551:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	1	49	theme	syndrome	169:176	arg1	coronavirus					178:188	severe acute respiratory syndrome coronavirus 2	144:190	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	144:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	1	49	theme	syndrome	169:176	arg1	SARS-CoV-2					193:202	SARS-CoV-2	193:202	SARS-CoV-2	193:202	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	7	50	theme	IgG	1436:1438	arg1	titers					1449:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	3	51	theme	response	564:571	arg1	development					536:546	the development	532:546	the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	532:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	4	52	theme	inflammatory	975:986	arg1	responses					988:996	inflammatory responses	975:996	inflammatory responses	975:996	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	1	53	theme	2019	109:112	arg1	pandemic					125:132	The coronavirus disease 2019 (COVID-19) pandemic	85:132	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	85:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	4	54	theme	animal	925:930	arg1	models					932:937	animal models	925:937	animal models	925:937	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	7	55	theme	anti-α-Gal	1411:1420	arg1	IgE					1422:1424	anti-α-Gal IgE	1411:1424	anti-α-Gal IgE	1411:1424	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	6	56	theme	healthy	1250:1256	arg1	individuals					1266:1276	healthy control individuals	1250:1276	healthy control individuals	1250:1276	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	1	57	theme	COVID-19	115:122	arg1	pandemic					125:132	The coronavirus disease 2019 (COVID-19) pandemic	85:132	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	85:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	0	58	theme	COVID-19	58:65	arg1	symptoms					75:82	COVID-19 disease symptoms	58:82	COVID-19 disease symptoms	58:82	The antibody response to the glycan α-Gal correlates with COVID-19 disease symptoms.					
33009829	5	59	theme	COVID-19	1097:1104	arg1	control					1086:1092	the control	1082:1092	the control of COVID-19	1082:1104	In this study, we hypothesized that the immune response to α-Gal may contribute to the control of COVID-19.					
33009829	3	60	theme	other	604:608	arg1	microorganisms					610:623	other microorganisms	604:623	other microorganisms	604:623	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	9	61	theme	α-Gal	1828:1832	arg1	epitopes					1834:1841	α-Gal epitopes	1828:1841	α-Gal epitopes	1828:1841	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	4	62	from	protection	911:920	arg1	models					932:937	animal models	925:937	animal models	925:937	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	7	63	from	alteration	1460:1469	arg1	titers					1449:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	6	64	from	stages	1208:1213	arg1	patients					1186:1193	patients	1186:1193	patients at different stages of COVID-19	1186:1225	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	6	64	from	stages	1208:1213	arg1	response					1165:1172	the antibody response	1152:1172	the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals	1152:1276	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	7	65	theme	IgG	1367:1369	arg1	titers					1380:1385	IgG antibody titers	1367:1385	IgG antibody titers	1367:1385	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	9	66	with	bacteria	1814:1821	arg1	epitopes					1834:1841	α-Gal epitopes	1828:1841	α-Gal epitopes	1828:1841	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	0	67	theme	antibody	4:11	arg1	response					13:20	The antibody response	0:20	The antibody response to the glycan α-Gal	0:40	The antibody response to the glycan α-Gal correlates with COVID-19 disease symptoms.					
33009829	7	68	from	reduction	1398:1406	arg1	titers					1449:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	anti-α-Gal IgE, IgM, and IgG antibody titers	1411:1454	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	9	69	theme	α-Gal-induced	1881:1893	arg1	response					1913:1920	α-Gal-induced protective immune response	1881:1920	α-Gal-induced protective immune response	1881:1920	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	1	70	theme	coronavirus	89:99	arg1	pandemic					125:132	The coronavirus disease 2019 (COVID-19) pandemic	85:132	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	85:203	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	9	71	theme	immune	1906:1911	arg1	response					1913:1920	α-Gal-induced protective immune response	1881:1920	α-Gal-induced protective immune response	1881:1920	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	8	72	theme	immune	1612:1617	arg1	response					1619:1626	the α-Gal-induced immune response	1594:1626	the α-Gal-induced immune response	1594:1626	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	3	73	theme	membrane	657:664	arg1	proteins					666:673	membrane proteins	657:673	membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	657:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	0	74	theme	glycan	29:34	arg1	α-Gal					36:40	the glycan α-Gal	25:40	the glycan α-Gal	25:40	The antibody response to the glycan α-Gal correlates with COVID-19 disease symptoms.					
33009829	3	75	theme	glycan	472:477	arg1	Galα1-3Galβ1-					479:491	the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	468:779	Galα1-3Galβ1-	479:491	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	7	76	theme	inflammatory	1313:1324	arg1	response					1326:1333	the inflammatory response	1309:1333	the inflammatory response	1309:1333	The results showed that while the inflammatory response and the anti-SARS-CoV-2 (Spike) IgG antibody titers increased, reduction in anti-α-Gal IgE, IgM, and IgG antibody titers and alteration of anti-α-Gal antibody isotype composition correlated with COVID-19 severity.					
33009829	3	77	theme	anti-α-Gal	687:696	arg1	antibodies					725:734	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies	687:734	anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota	687:779	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	6	78	theme	antibody	1156:1163	arg1	response					1165:1172	the antibody response	1152:1172	the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals	1152:1276	To address this hypothesis, we characterized the antibody response to α-Gal in patients at different stages of COVID-19 and in comparison with healthy control individuals.					
33009829	9	79	theme	COVID-19	1945:1952	arg1	severity					1933:1940	severity	1933:1940	severity of COVID-19	1933:1952	These results support the proposal of developing interventions such as probiotics based on commensal bacteria with α-Gal epitopes to modify the microbiota and increase α-Gal-induced protective immune response and reduce severity of COVID-19.					
33009829	8	80	theme	aggressive	1652:1661	arg1	viremia					1663:1669	more aggressive viremia	1647:1669	more aggressive viremia	1647:1669	The results suggested that the inhibition of the α-Gal-induced immune response may translate into more aggressive viremia and severe disease inflammatory symptoms.					
33009829	4	81	theme	anti-α-Gal	797:806	arg1	opsonization					835:846	anti-α-Gal antibody-mediated pathogen opsonization	797:846	anti-α-Gal antibody-mediated pathogen opsonization	797:846	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	1	82	theme	affected	209:216	arg1	millions					218:225	affected millions	209:225	affected millions of people worldwide	209:245	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide.					
33009829	3	83	dep	Galα1-3Galβ1-	479:491	arg1	4GlcNAc-R					495:503	4GlcNAc-R	495:503	4GlcNAc-R (α-Gal)	495:511	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	3	83	dep	Galα1-3Galβ1-	479:491	arg1	3					493:493	3	493:493	3	493:493	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	3	83	dep	Galα1-3Galβ1-	479:491	arg1	α-Gal					506:510	α-Gal	506:510	α-Gal	506:510	Humans evolved by losing the capacity to synthesize the glycan Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), which resulted in the development of a protective response against pathogenic viruses and other microorganisms containing this modification on membrane proteins mediated by anti-α-Gal immunoglobulin M (IgM)/IgG antibodies produced in response to bacterial microbiota.					
33009829	4	84	theme	pathogen	826:833	arg1	opsonization					835:846	anti-α-Gal antibody-mediated pathogen opsonization	797:846	anti-α-Gal antibody-mediated pathogen opsonization	797:846	In addition to anti-α-Gal antibody-mediated pathogen opsonization, this glycan induces various immune mechanisms that have shown protection in animal models against infectious diseases without inflammatory responses.					
33009829	2	85	theme	progress in	372:382	arg1	control					392:398	progress in disease control	372:398	progress in disease control	372:398	Characterization of the immunological mechanisms involved in disease symptomatology and protective response is important to progress in disease control and prevention.					
33587947	2	0	theme	Langmuir	463:470	arg1	adsorption					472:481	the Langmuir adsorption	459:481	the Langmuir adsorption	459:481	Kinetic and thermodynamics revealed that the Cd(II) adsorption onto MgO-BCR-W was well fitted by pseudo-second-order and the Langmuir adsorption isotherm.					
33587947	4	1	theme	RL	657:658	arg1	values					647:652	the computed values	634:652	the computed values of RL	634:658	Attractively, the computed values of RL ranged between 0 and 1, suggesting that the adsorption of Cd(II) onto MgO-BCR-W is favourable.					
33587947	6	2	from	effective	1008:1016	arg1	stabilization					1024:1036	Cd stabilization	1021:1036	Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%	1021:1116	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	6	2	from	effective	1008:1016	arg1	experiment					942:951	soil incubation experiment	926:951	soil incubation experiment	926:951	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	1	3	theme	magnesium	135:143	arg1	adsorbent					267:275	adsorbent	267:275	adsorbent	267:275	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	3	theme	magnesium	135:143	arg1	MgO-BCR-W					179:187	MgO-BCR-W	179:187	MgO-BCR-W	179:187	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	3	theme	magnesium	135:143	arg1	composite					168:176	magnesium oxide biochar-chitosan composite	135:176	magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan	135:242	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	6	4	theme	incubation	931:940	arg1	experiment					942:951	soil incubation experiment	926:951	soil incubation experiment	926:951	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	1	5	with	modification	207:218	arg1	chitosan					235:242	chitosan	235:242	chitosan	235:242	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	5	with	modification	207:218	arg1	MgCl2					225:229	MgCl2	225:229	MgCl2	225:229	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	6	theme	soil	320:323	arg1	environment					325:335	the aquatic and soil environment	304:335	environment	325:335	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	6	7	from	experiment	942:951	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	6	7	from	experiment	942:951	arg1	addition					958:965	the addition	954:965	the addition of MgO-BCR-W at the level of 2%	954:997	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	5	8	theme	surface	831:837	arg1	complexation					839:850	surface complexation	831:850	surface complexation	831:850	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	1	9	theme	oxide	145:149	arg1	adsorbent					267:275	adsorbent	267:275	adsorbent	267:275	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	9	theme	oxide	145:149	arg1	MgO-BCR-W					179:187	MgO-BCR-W	179:187	MgO-BCR-W	179:187	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	9	theme	oxide	145:149	arg1	composite					168:176	magnesium oxide biochar-chitosan composite	135:176	magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan	135:242	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	6	10	theme	soil	926:929	arg1	experiment					942:951	soil incubation experiment	926:951	soil incubation experiment	926:951	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	7	11	theme	Cd	1207:1208	arg1	content					1210:1216	the residual Cd content	1194:1216	the residual Cd content	1194:1216	Furthermore, it reduced the acid extractable Cd by 24.77%, while increased the residual Cd content by 22.24%.					
33587947	6	12	from	level	987:991	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	6	12	from	level	987:991	arg1	addition					958:965	the addition	954:965	the addition of MgO-BCR-W at the level of 2%	954:997	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	3	13	theme	husk	527:530	arg1	BCR					541:543	BCR	541:543	BCR	541:543	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	3	13	theme	husk	527:530	arg1	biochar					532:538	rice husk biochar	522:538	rice husk biochar (BCR)	522:544	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	0	14	theme	in-situ	75:81	arg1	remediation					83:93	its in-situ remediation	71:93	its in-situ remediation of Cd-contaminated soil	71:117	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	2	15	dep	pseudo-second-order	435:453	arg1	isotherm					483:490	isotherm	483:490	isotherm	483:490	Kinetic and thermodynamics revealed that the Cd(II) adsorption onto MgO-BCR-W was well fitted by pseudo-second-order and the Langmuir adsorption isotherm.					
33587947	3	16	theme	biochar	532:538	arg1	capacities					508:517	The adsorption capacities	493:517	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II)	493:569	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	0	17	theme	Cd-contaminated	98:112	arg1	soil					114:117	Cd-contaminated soil	98:117	Cd-contaminated soil	98:117	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	6	18	theme	%	997:997	arg1	level					987:991	the level	983:991	the level of 2%	983:997	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	4	19	theme	Cd	718:719	arg1	adsorption					704:713	the adsorption	700:713	the adsorption of Cd(II) onto MgO-BCR-W	700:738	Attractively, the computed values of RL ranged between 0 and 1, suggesting that the adsorption of Cd(II) onto MgO-BCR-W is favourable.					
33587947	4	19	theme	Cd	718:719	arg1	favourable					743:752	favourable	743:752	favourable	743:752	Attractively, the computed values of RL ranged between 0 and 1, suggesting that the adsorption of Cd(II) onto MgO-BCR-W is favourable.					
33587947	1	20	theme	biochar-chitosan	151:166	arg1	adsorbent					267:275	adsorbent	267:275	adsorbent	267:275	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	20	theme	biochar-chitosan	151:166	arg1	MgO-BCR-W					179:187	MgO-BCR-W	179:187	MgO-BCR-W	179:187	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	1	20	theme	biochar-chitosan	151:166	arg1	composite					168:176	magnesium oxide biochar-chitosan composite	135:176	magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan	135:242	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	8	21	from	remediation	1333:1343	arg1	soil					1363:1366	soil	1363:1366	soil	1363:1366	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	8	21	from	remediation	1333:1343	arg1	water					1353:1357	water	1353:1357	water	1353:1357	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	0	22	from	solution	27:34	arg1	Removal					0:6	Removal	0:6	Removal of Cd from aqueous solution by chitosan	0:46	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	3	23	theme	rice	522:525	arg1	BCR					541:543	BCR	541:543	BCR	541:543	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	3	23	theme	rice	522:525	arg1	biochar					532:538	rice husk biochar	522:538	rice husk biochar (BCR)	522:544	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	8	24	dep	water	1353:1357	arg1	environment					1368:1378	environment	1368:1378	environment	1368:1378	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	5	25	theme	major	890:894	arg1	role					896:899	a major role	888:899	a major role	888:899	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	5	26	theme	synergistic	809:819	arg1	effect					821:826	the synergistic effect	805:826	the synergistic effect of surface complexation and precipitation mechanisms	805:879	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	0	27	theme	Cd	11:12	arg1	Removal					0:6	Removal	0:6	Removal of Cd from aqueous solution by chitosan	0:46	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	0	28	theme	soil	114:117	arg1	remediation					83:93	its in-situ remediation	71:93	its in-situ remediation of Cd-contaminated soil	71:117	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	0	28	theme	soil	114:117	arg1	MgO-biochar					55:65	MgO-biochar	55:65	MgO-biochar	55:65	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	8	29	theme	adsorbent	1316:1324	arg1	MgO-BCR-W					1259:1267	MgO-BCR-W	1259:1267	MgO-BCR-W	1259:1267	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	8	29	theme	adsorbent	1316:1324	arg1	remediation					1333:1343	an effective and eco-friendly adsorbent for Cd remediation	1286:1343	an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment	1286:1378	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	5	30	theme	complexation	839:850	arg1	effect					821:826	the synergistic effect	805:826	the synergistic effect of surface complexation and precipitation mechanisms	805:879	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	8	31	used	used	1278:1281	arg2	MgO-BCR-W					1259:1267	MgO-BCR-W	1259:1267	MgO-BCR-W	1259:1267	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	8	31	used	used	1278:1281	arg2	remediation					1333:1343	an effective and eco-friendly adsorbent for Cd remediation	1286:1343	an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment	1286:1378	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	7	32	theme	residual	1198:1205	arg1	content					1210:1216	the residual Cd content	1194:1216	the residual Cd content	1194:1216	Furthermore, it reduced the acid extractable Cd by 24.77%, while increased the residual Cd content by 22.24%.					
33587947	6	33	theme	MgO-BCR-W	970:978	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	6	33	theme	MgO-BCR-W	970:978	arg1	addition					958:965	the addition	954:965	the addition of MgO-BCR-W at the level of 2%	954:997	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	0	34	theme	aqueous	19:25	arg1	solution					27:34	aqueous solution	19:34	aqueous solution	19:34	Removal of Cd from aqueous solution by chitosan coated MgO-biochar and its in-situ remediation of Cd-contaminated soil.					
33587947	1	35	from	environment	325:335	arg1	removal					285:291	the removal	281:291	the removal of Cd from the aquatic and soil environment	281:335	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	6	36	from	stabilization	1024:1036	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	6	36	from	stabilization	1024:1036	arg1	addition					958:965	the addition	954:965	the addition of MgO-BCR-W at the level of 2%	954:997	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	5	37	theme	adsorbents	780:789	arg1	Characterisations					755:771	Characterisations	755:771	Characterisations of the adsorbents	755:789	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	1	38	theme	Cd	296:297	arg1	removal					285:291	the removal	281:291	the removal of Cd from the aquatic and soil environment	281:335	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	3	39	theme	MgO-BCR-W	550:558	arg1	capacities					508:517	The adsorption capacities	493:517	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II)	493:569	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	5	40	dep	complexation	839:850	arg1	mechanisms					870:879	mechanisms	870:879	mechanisms	870:879	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	2	41	theme	Cd	383:384	arg1	adsorption					390:399	the Cd(II) adsorption	379:399	the Cd(II) adsorption onto MgO-BCR-W	379:414	Kinetic and thermodynamics revealed that the Cd(II) adsorption onto MgO-BCR-W was well fitted by pseudo-second-order and the Langmuir adsorption isotherm.					
33587947	8	42	theme	effective	1289:1297	arg1	MgO-BCR-W					1259:1267	MgO-BCR-W	1259:1267	MgO-BCR-W	1259:1267	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	8	42	theme	effective	1289:1297	arg1	remediation					1333:1343	an effective and eco-friendly adsorbent for Cd remediation	1286:1343	an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment	1286:1378	The results demonstrated that MgO-BCR-W could be used as an effective and eco-friendly adsorbent for Cd remediation in both water and soil environment.					
33587947	5	43	theme	precipitation	856:868	arg1	effect					821:826	the synergistic effect	805:826	the synergistic effect of surface complexation and precipitation mechanisms	805:879	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	7	44	theme	acid	1147:1150	arg1	Cd					1164:1165	the acid extractable Cd	1143:1165	the acid extractable Cd	1143:1165	Furthermore, it reduced the acid extractable Cd by 24.77%, while increased the residual Cd content by 22.24%.					
33587947	6	45	theme	22.32	1111:1115	arg1	%					1116:1116	22.32%	1111:1116	22.32%	1111:1116	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	7	46	theme	extractable	1152:1162	arg1	Cd					1164:1165	the acid extractable Cd	1143:1165	the acid extractable Cd	1143:1165	Furthermore, it reduced the acid extractable Cd by 24.77%, while increased the residual Cd content by 22.24%.					
33587947	5	47	theme	Cd	919:920	arg1	removal					908:914	the removal	904:914	the removal of Cd	904:920	Characterisations of the adsorbents revealed that the synergistic effect of surface complexation and precipitation mechanisms played a major role in the removal of Cd.					
33587947	6	48	theme	Cd	1021:1022	arg1	stabilization					1024:1036	Cd stabilization	1021:1036	Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%	1021:1116	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	6	49	theme	Cd	1105:1106	arg1	content					1081:1087	the content	1077:1087	the content of bioavailable Cd	1077:1106	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	3	50	theme	adsorption	497:506	arg1	capacities					508:517	The adsorption capacities	493:517	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II)	493:569	The adsorption capacities of rice husk biochar (BCR) and MgO-BCR-W for Cd(II) reached 11.09 mg/g and 59.66 mg/g, respectively.					
33587947	1	51	theme	aquatic	308:314	arg1	environment					325:335	the aquatic and soil environment	304:335	environment	325:335	In this study, magnesium oxide biochar-chitosan composite (MgO-BCR-W) prepared through modification with MgCl2 and chitosan was investigated as an adsorbent for the removal of Cd from the aquatic and soil environment.					
33587947	6	52	theme	bioavailable	1092:1103	arg1	Cd					1105:1106	bioavailable Cd	1092:1106	bioavailable Cd	1092:1106	In soil incubation experiment, the addition of MgO-BCR-W at the level of 2% was most effective in Cd stabilization compared to the control, which reduced the content of bioavailable Cd by 22.32%.					
33587947	4	53	theme	computed	638:645	arg1	values					647:652	the computed values	634:652	the computed values of RL	634:658	Attractively, the computed values of RL ranged between 0 and 1, suggesting that the adsorption of Cd(II) onto MgO-BCR-W is favourable.					
33094479	13	0	theme	Occlusal	1725:1732	arg1	trauma					1734:1739	Occlusal trauma	1725:1739	Occlusal trauma	1725:1739	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	6	1	theme	occlusal	923:930	arg1	trauma					932:937	occlusal trauma	923:937	occlusal trauma	923:937	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	3	2	dep	METHODS	307:313	arg1	reaction					372:379	quantitative real-time polymerase chain reaction	332:379	quantitative real-time polymerase chain reaction (qRT-PCR)	332:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	2	dep	METHODS	307:313	arg1	qRT-PCR					382:388	qRT-PCR	382:388	qRT-PCR	382:388	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	2	dep	METHODS	307:313	arg1	METHODS					307:313	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	2	dep	METHODS	307:313	arg1	blot					323:326	Western blot	315:326	Western blot	315:326	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	9	3	theme	increased	1181:1189	arg1	RESULTS					1138:1144	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	3	theme	increased	1181:1189	arg1	expression					1191:1200	increased expression	1181:1200	increased expression of inflammatory factors	1181:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	10	4	theme	N-terminal	1414:1423	arg1	JNK					1434:1436	JNK	1434:1436	JNK	1434:1436	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	10	4	theme	N-terminal	1414:1423	arg1	kinases					1425:1431	Jun N-terminal kinases	1410:1431	Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation	1410:1484	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	4	5	theme	periodontitis	666:678	arg1	models					700:705	periodontitis and occlusal trauma models	666:705	models	700:705	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	3	6	with	patients	464:471	arg1	periodontitis					478:490	periodontitis	478:490	periodontitis accompanied with or without occlusal trauma	478:534	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	10	7	theme	periodontitis	1327:1339	arg1	model					1318:1322	the mouse model	1308:1322	the mouse model of periodontitis with occlusal trauma	1308:1360	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	12	8	theme	JNK	1644:1646	arg1	expression					1680:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	4	9	theme	occlusal	684:691	arg1	models					700:705	periodontitis and occlusal trauma models	666:705	models	700:705	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	5	10	theme	YAP	753:755	arg1	activation					757:766	YAP activation	753:766	YAP activation	753:766	Treatment with or without XAV939, to inhibit YAP activation, was performed in these models.					
33094479	7	11	theme	cell	1033:1036	arg1	line					1038:1041	the L929 mouse fibroblast cell line	1007:1041	the L929 mouse fibroblast cell line	1007:1041	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	3	12	theme	Western	315:321	arg1	METHODS					307:313	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	12	theme	Western	315:321	arg1	blot					323:326	Western blot	315:326	Western blot	315:326	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	2	13	theme	mechanical	234:243	arg1	protein					254:260	a mechanical stressor protein	232:260	a mechanical stressor protein	232:260	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	2	13	theme	mechanical	234:243	arg1	protein					217:223	Yes-associated protein	202:223	Yes-associated protein (YAP)	202:229	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	7	14	theme	mouse	1016:1020	arg1	line					1038:1041	the L929 mouse fibroblast cell line	1007:1041	the L929 mouse fibroblast cell line	1007:1041	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	12	15	theme	protein	1606:1612	arg1	dephosphorylation					1614:1630	YAP protein dephosphorylation	1602:1630	YAP protein dephosphorylation	1602:1630	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	3	16	theme	real-time	345:353	arg1	reaction					372:379	quantitative real-time polymerase chain reaction	332:379	quantitative real-time polymerase chain reaction (qRT-PCR)	332:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	16	theme	real-time	345:353	arg1	METHODS					307:313	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	16	theme	real-time	345:353	arg1	qRT-PCR					382:388	qRT-PCR	382:388	qRT-PCR	382:388	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	11	17	theme	cyclic	1497:1502	arg1	stress					1504:1509	L929 cell cyclic stress	1487:1509	L929 cell cyclic stress	1487:1509	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	6	18	used	used	869:872	arg2	immunofluorescence					827:844	immunofluorescence	827:844	immunofluorescence (IF)	827:849	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	6	18	used	used	869:872	arg2	qRT-PCR					856:862	qRT-PCR	856:862	qRT-PCR	856:862	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	6	18	used	used	869:872	arg2	tomography					815:824	Micro-computed tomography	800:824	Micro-computed tomography	800:824	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	14	19	theme	XAV939	1873:1878	arg1	inhibitor					1884:1892	the XAV939 YAP inhibitor	1869:1892	the XAV939 YAP inhibitor	1869:1892	This can be inhibited by the XAV939 YAP inhibitor.					
33094479	6	20	from	pathway	893:899	arg1	periodontitis					904:916	periodontitis	904:916	periodontitis	904:916	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	10	21	theme	pro-inflammatory	1447:1462	arg1	up-regulation					1472:1484	pro-inflammatory pathway up-regulation	1447:1484	pro-inflammatory pathway up-regulation	1447:1484	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	3	22	from	expression	418:427	arg1	patients					464:471	patients	464:471	patients with periodontitis accompanied with or without occlusal trauma	464:534	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	13	23	theme	YAP	1754:1756	arg1	transfer					1766:1773	YAP nuclear transfer	1754:1773	YAP nuclear transfer	1754:1773	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	11	24	theme	stimulation	1519:1529	arg1	results					1531:1537	L929 cell cyclic stress and LPS stimulation results	1487:1537	L929 cell cyclic stress and LPS stimulation results	1487:1537	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	3	25	theme	chain	366:370	arg1	reaction					372:379	quantitative real-time polymerase chain reaction	332:379	quantitative real-time polymerase chain reaction (qRT-PCR)	332:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	25	theme	chain	366:370	arg1	METHODS					307:313	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	25	theme	chain	366:370	arg1	qRT-PCR					382:388	qRT-PCR	382:388	qRT-PCR	382:388	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	26	theme	inflammatory	440:451	arg1	factors					453:459	inflammatory factors	440:459	inflammatory factors	440:459	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	4	27	from	bonding	614:620	arg1	molars					635:640	maxillary molars	625:640	maxillary molars in mice	625:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	7	28	theme	lipopolysaccharide	958:975	arg1	stimuli					983:989	Cyclic stress and lipopolysaccharide (LPS) stimuli	940:989	Cyclic stress and lipopolysaccharide (LPS) stimuli	940:989	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	8	29	dep	in	1121:1122	arg1	vivo					1124:1127	vivo	1124:1127	vivo	1124:1127	Western blot, IF, and qRT-PCR were used to verify the in vivo results.					
33094479	3	30	theme	YAP	432:434	arg1	expression					418:427	the expression	414:427	the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma	414:534	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	7	31	theme	stress	947:952	arg1	stimuli					983:989	Cyclic stress and lipopolysaccharide (LPS) stimuli	940:989	Cyclic stress and lipopolysaccharide (LPS) stimuli	940:989	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	1	32	theme	Occlusal	122:129	arg1	trauma					131:136	BACKGROUND Occlusal trauma	111:136	BACKGROUND Occlusal trauma	111:136	BACKGROUND Occlusal trauma can aggravate periodontitis, but the mechanism remains unclear.					
33094479	12	33	theme	factor	1673:1678	arg1	expression					1680:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	4	34	theme	gingivalis	583:592	arg1	administration					551:564	local administration	545:564	local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice	545:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	11	35	theme	L929	1487:1490	arg1	stress					1504:1509	L929 cell cyclic stress	1487:1509	L929 cell cyclic stress	1487:1509	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	0	36	theme	yes-associated	14:27	arg1	dephosphorylation					37:53	yes-associated protein dephosphorylation	14:53	yes-associated protein dephosphorylation	14:53	Inhibition of yes-associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma.					
33094479	6	37	theme	YAP	889:891	arg1	pathway					893:899	the YAP pathway	885:899	the YAP pathway in periodontitis with occlusal trauma	885:937	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	4	38	theme	composite	598:606	arg1	bonding					614:620	composite resin bonding	598:620	composite resin bonding	598:620	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	10	39	theme	mouse	1312:1316	arg1	model					1318:1322	the mouse model	1308:1322	the mouse model of periodontitis with occlusal trauma	1308:1360	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	0	40	theme	dephosphorylation	37:53	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of yes-associated protein dephosphorylation	0:53	Inhibition of yes-associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma.					
33094479	4	41	theme	local	545:549	arg1	administration					551:564	local administration	545:564	local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice	545:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	10	42	dep	related	1439:1445	arg1	up-regulation					1472:1484	pro-inflammatory pathway up-regulation	1447:1484	pro-inflammatory pathway up-regulation	1447:1484	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	7	43	theme	Cyclic	940:945	arg1	stress					947:952	Cyclic stress	940:952	Cyclic stress	940:952	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	6	44	with	pathway	893:899	arg1	trauma					932:937	occlusal trauma	923:937	occlusal trauma	923:937	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	9	45	theme	dephosphorylated	1156:1171	arg1	RESULTS					1138:1144	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	45	theme	dephosphorylated	1156:1171	arg1	YAP					1173:1175	Activated dephosphorylated YAP	1146:1175	Activated dephosphorylated YAP	1146:1175	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	3	46	theme	occlusal	520:527	arg1	trauma					529:534	occlusal trauma	520:534	occlusal trauma	520:534	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	13	47	theme	pro-inflammatory	1818:1833	arg1	pathway					1835:1841	the JNK pro-inflammatory pathway	1810:1841	the JNK pro-inflammatory pathway	1810:1841	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	4	48	theme	bonding	614:620	arg1	administration					551:564	local administration	545:564	local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice	545:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	13	49	theme	JNK	1814:1816	arg1	pathway					1835:1841	the JNK pro-inflammatory pathway	1810:1841	the JNK pro-inflammatory pathway	1810:1841	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	4	50	theme	maxillary	625:633	arg1	molars					635:640	maxillary molars	625:640	maxillary molars in mice	625:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	13	51	theme	pathway	1835:1841	arg1	up-regulation					1793:1805	the up-regulation	1789:1805	the up-regulation of the JNK pro-inflammatory pathway	1789:1841	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	11	52	theme	in	1553:1554	arg1	results					1561:1567	the in vivo results	1549:1567	the in vivo results	1549:1567	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	0	53	theme	aggravated	64:73	arg1	periodontitis					75:87	aggravated periodontitis	64:87	aggravated periodontitis with occlusal trauma	64:108	Inhibition of yes-associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma.					
33094479	4	54	theme	trauma	693:698	arg1	models					700:705	periodontitis and occlusal trauma models	666:705	models	700:705	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	9	55	theme	inflammatory	1205:1216	arg1	factors					1218:1224	inflammatory factors	1205:1224	inflammatory factors	1205:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	12	56	theme	reduced	1636:1642	arg1	expression					1680:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	9	57	theme	factors	1218:1224	arg1	RESULTS					1138:1144	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	57	theme	factors	1218:1224	arg1	YAP					1173:1175	Activated dephosphorylated YAP	1146:1175	Activated dephosphorylated YAP	1146:1175	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	57	theme	factors	1218:1224	arg1	expression					1191:1200	increased expression	1181:1200	increased expression of inflammatory factors	1181:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	6	58	theme	Micro-computed	800:813	arg1	tomography					815:824	Micro-computed tomography	800:824	Micro-computed tomography	800:824	Micro-computed tomography, immunofluorescence (IF), and qRT-PCR were used to explore the YAP pathway in periodontitis with occlusal trauma.					
33094479	12	59	theme	pro-inflammatory	1648:1663	arg1	expression					1680:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	12	60	theme	YAP	1602:1604	arg1	dephosphorylation					1614:1630	YAP protein dephosphorylation	1602:1630	YAP protein dephosphorylation	1602:1630	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	2	61	theme	stressor	245:252	arg1	protein					254:260	a mechanical stressor protein	232:260	a mechanical stressor protein	232:260	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	2	61	theme	stressor	245:252	arg1	protein					217:223	Yes-associated protein	202:223	Yes-associated protein (YAP)	202:229	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	13	62	dep	CONCLUSIONS	1713:1723	arg1	activate					1745:1752	activate	1745:1752	activate	1745:1752	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	11	63	theme	stress	1504:1509	arg1	results					1531:1537	L929 cell cyclic stress and LPS stimulation results	1487:1537	L929 cell cyclic stress and LPS stimulation results	1487:1537	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	10	64	theme	Jun	1410:1412	arg1	JNK					1434:1436	JNK	1434:1436	JNK	1434:1436	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	10	64	theme	Jun	1410:1412	arg1	kinases					1425:1431	Jun N-terminal kinases	1410:1431	Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation	1410:1484	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	2	65	theme	important	275:283	arg1	role					285:288	an important role	272:288	an important role	272:288	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	7	66	theme	fibroblast	1022:1031	arg1	line					1038:1041	the L929 mouse fibroblast cell line	1007:1041	the L929 mouse fibroblast cell line	1007:1041	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	11	67	theme	cell	1492:1495	arg1	stress					1504:1509	L929 cell cyclic stress	1487:1509	L929 cell cyclic stress	1487:1509	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	0	68	theme	occlusal	94:101	arg1	trauma					103:108	occlusal trauma	94:108	occlusal trauma	94:108	Inhibition of yes-associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma.					
33094479	9	69	theme	occlusal	1288:1295	arg1	trauma					1297:1302	occlusal trauma	1288:1302	occlusal trauma	1288:1302	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	7	70	theme	L929	1011:1014	arg1	line					1038:1041	the L929 mouse fibroblast cell line	1007:1041	the L929 mouse fibroblast cell line	1007:1041	Cyclic stress and lipopolysaccharide (LPS) stimuli were applied to the L929 mouse fibroblast cell line with or without XAV939.					
33094479	14	71	theme	YAP	1880:1882	arg1	inhibitor					1884:1892	the XAV939 YAP inhibitor	1869:1892	the XAV939 YAP inhibitor	1869:1892	This can be inhibited by the XAV939 YAP inhibitor.					
33094479	9	72	located	observed	1231:1238	arg1	patients					1243:1250	patients	1243:1250	patients with periodontitis accompanied with occlusal trauma	1243:1302	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	72	located	observed	1231:1238	arg2	YAP					1173:1175	Activated dephosphorylated YAP	1146:1175	Activated dephosphorylated YAP	1146:1175	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	72	located	observed	1231:1238	arg2	RESULTS					1138:1144	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	72	located	observed	1231:1238	arg2	expression					1191:1200	increased expression	1181:1200	increased expression of inflammatory factors	1181:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	12	73	theme	XAV939	1585:1590	arg1	Application					1570:1580	Application	1570:1580	Application of XAV939	1570:1590	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	3	74	theme	polymerase	355:364	arg1	reaction					372:379	quantitative real-time polymerase chain reaction	332:379	quantitative real-time polymerase chain reaction (qRT-PCR)	332:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	74	theme	polymerase	355:364	arg1	METHODS					307:313	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	74	theme	polymerase	355:364	arg1	qRT-PCR					382:388	qRT-PCR	382:388	qRT-PCR	382:388	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	75	theme	quantitative	332:343	arg1	reaction					372:379	quantitative real-time polymerase chain reaction	332:379	quantitative real-time polymerase chain reaction (qRT-PCR)	332:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	75	theme	quantitative	332:343	arg1	METHODS					307:313	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)	307:389	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	3	75	theme	quantitative	332:343	arg1	qRT-PCR					382:388	qRT-PCR	382:388	qRT-PCR	382:388	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	10	76	theme	related	1439:1445	arg1	JNK					1434:1436	JNK	1434:1436	JNK	1434:1436	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	10	76	theme	related	1439:1445	arg1	kinases					1425:1431	Jun N-terminal kinases	1410:1431	Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation	1410:1484	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	11	77	theme	LPS	1515:1517	arg1	stimulation					1519:1529	LPS stimulation	1515:1529	LPS stimulation	1515:1529	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	4	78	from	molars	635:640	arg1	mice					645:648	mice	645:648	mice	645:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	4	78	from	molars	635:640	arg1	administration					551:564	local administration	545:564	local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice	545:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	13	79	theme	nuclear	1758:1764	arg1	transfer					1766:1773	YAP nuclear transfer	1754:1773	YAP nuclear transfer	1754:1773	CONCLUSIONS Occlusal trauma can activate YAP nuclear transfer, resulting in the up-regulation of the JNK pro-inflammatory pathway.					
33094479	8	80	theme	Western	1067:1073	arg1	blot					1075:1078	Western blot	1067:1078	Western blot, IF,	1067:1083	Western blot, IF, and qRT-PCR were used to verify the in vivo results.					
33094479	3	81	theme	factors	453:459	arg1	expression					418:427	the expression	414:427	the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma	414:534	METHODS Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the expression of YAP and inflammatory factors in patients with periodontitis accompanied with or without occlusal trauma.					
33094479	10	82	with	periodontitis	1327:1339	arg1	trauma					1355:1360	occlusal trauma	1346:1360	occlusal trauma	1346:1360	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	1	83	theme	BACKGROUND	111:120	arg1	trauma					131:136	BACKGROUND Occlusal trauma	111:136	BACKGROUND Occlusal trauma	111:136	BACKGROUND Occlusal trauma can aggravate periodontitis, but the mechanism remains unclear.					
33094479	10	84	theme	pathway	1464:1470	arg1	up-regulation					1472:1484	pro-inflammatory pathway up-regulation	1447:1484	pro-inflammatory pathway up-regulation	1447:1484	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	9	85	with	patients	1243:1250	arg1	periodontitis					1257:1269	periodontitis	1257:1269	periodontitis accompanied with occlusal trauma	1257:1302	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	0	86	theme	protein	29:35	arg1	dephosphorylation					37:53	yes-associated protein dephosphorylation	14:53	yes-associated protein dephosphorylation	14:53	Inhibition of yes-associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma.					
33094479	12	87	theme	pathway	1665:1671	arg1	expression					1680:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	reduced JNK pro-inflammatory pathway factor expression	1636:1689	Application of XAV939 inhibited YAP protein dephosphorylation and reduced JNK pro-inflammatory pathway factor expression in vivo and in vitro.					
33094479	4	88	theme	resin	608:612	arg1	bonding					614:620	composite resin bonding	598:620	composite resin bonding	598:620	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	9	89	dep	RESULTS	1138:1144	arg1	RESULTS					1138:1144	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	89	dep	RESULTS	1138:1144	arg1	YAP					1173:1175	Activated dephosphorylated YAP	1146:1175	Activated dephosphorylated YAP	1146:1175	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	89	dep	RESULTS	1138:1144	arg1	expression					1191:1200	increased expression	1181:1200	increased expression of inflammatory factors	1181:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	2	90	theme	Yes-associated	202:215	arg1	protein					217:223	Yes-associated protein	202:223	Yes-associated protein (YAP)	202:229	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	2	90	theme	Yes-associated	202:215	arg1	protein					254:260	a mechanical stressor protein	232:260	a mechanical stressor protein	232:260	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	2	90	theme	Yes-associated	202:215	arg1	YAP					226:228	YAP	226:228	YAP	226:228	Yes-associated protein (YAP), a mechanical stressor protein, may play an important role in this process.					
33094479	4	91	from	administration	551:564	arg1	molars					635:640	maxillary molars	625:640	maxillary molars in mice	625:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	8	92	used	used	1102:1105	arg2	blot					1075:1078	Western blot	1067:1078	Western blot, IF,	1067:1083	Western blot, IF, and qRT-PCR were used to verify the in vivo results.					
33094479	8	92	used	used	1102:1105	arg2	qRT-PCR					1089:1095	qRT-PCR	1089:1095	qRT-PCR	1089:1095	Western blot, IF, and qRT-PCR were used to verify the in vivo results.					
33094479	4	93	theme	Porphyromonas	569:581	arg1	gingivalis					583:592	Porphyromonas gingivalis	569:592	Porphyromonas gingivalis	569:592	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	10	94	theme	occlusal	1346:1353	arg1	trauma					1355:1360	occlusal trauma	1346:1360	occlusal trauma	1346:1360	In the mouse model of periodontitis with occlusal trauma, YAP transferred into the nucleus, resulting in Jun N-terminal kinases (JNK) related pro-inflammatory pathway up-regulation.					
33094479	11	95	dep	in	1553:1554	arg1	vivo					1556:1559	vivo	1556:1559	vivo	1556:1559	L929 cell cyclic stress and LPS stimulation results confirmed the in vivo results.					
33094479	4	96	from	gingivalis	583:592	arg1	molars					635:640	maxillary molars	625:640	maxillary molars in mice	625:648	Through local administration of Porphyromonas gingivalis and composite resin bonding on maxillary molars in mice, we established periodontitis and occlusal trauma models.					
33094479	0	97	with	periodontitis	75:87	arg1	trauma					103:108	occlusal trauma	94:108	occlusal trauma	94:108	Inhibition of yes-associated protein dephosphorylation prevents aggravated periodontitis with occlusal trauma.					
33094479	8	98	theme	in	1121:1122	arg1	results					1129:1135	the in vivo results	1117:1135	the in vivo results	1117:1135	Western blot, IF, and qRT-PCR were used to verify the in vivo results.					
33094479	9	99	theme	Activated	1146:1154	arg1	RESULTS					1138:1144	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors	1138:1224	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33094479	9	99	theme	Activated	1146:1154	arg1	YAP					1173:1175	Activated dephosphorylated YAP	1146:1175	Activated dephosphorylated YAP	1146:1175	RESULTS Activated dephosphorylated YAP and increased expression of inflammatory factors were observed in patients with periodontitis accompanied with occlusal trauma.					
33482208	0	0	theme	hypocholesterolemic	72:90	arg1	potential					92:100	in vitro and in vivo hypocholesterolemic potential	51:100	in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	51:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	4	1	theme	composition	532:542	arg1	Analysis					501:508	Analysis	501:508	Analysis of the monosaccharide composition by thin layer chromatography	501:571	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	9	2	with	chromatography	1379:1392	arg1	activities					1418:1427	good antimicrobial activities	1399:1427	good antimicrobial activities	1399:1427	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	11	3	theme	atherogenic	1658:1668	arg1	indices					1670:1676	the atherogenic indices	1654:1676	the atherogenic indices	1654:1676	Whereas, HDL was increased by about 43 and 38%, respectively, and the atherogenic indices decreased.					
33482208	2	4	from	examples	308:315	arg1	industry					372:379	the food industry	363:379	the food industry	363:379	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	4	5	theme	unknown	619:625	arg1	sugar					627:631	unknown sugar	619:631	unknown sugar	619:631	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	7	6	theme	HCT-116	1083:1089	arg1	cells					1108:1112	HCT-116, PC-3 and HepG-2 cells	1083:1112	cells	1108:1112	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	4	7	theme	layer	552:556	arg1	chromatography					558:571	thin layer chromatography	547:571	thin layer chromatography	547:571	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	5	8	theme	Fourier-Transform	688:704	arg1	Spectroscopy					715:726	Fourier-Transform Infrared Spectroscopy	688:726	Fourier-Transform Infrared Spectroscopy	688:726	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	2	9	theme	food	367:370	arg1	industry					372:379	the food industry	363:379	the food industry	363:379	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	6	10	dep	%	939:939	arg1	to					934:935	to	934:935	to	934:935	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	4	11	theme	thin	547:550	arg1	chromatography					558:571	thin layer chromatography	547:571	thin layer chromatography	547:571	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	5	12	theme	Infrared	706:713	arg1	Spectroscopy					715:726	Fourier-Transform Infrared Spectroscopy	688:726	Fourier-Transform Infrared Spectroscopy	688:726	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	5	13	theme	main	638:641	arg1	EPSs					664:667	EPSs	664:667	EPSs	664:667	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	5	13	theme	main	638:641	arg1	groups					654:659	The main functional groups	634:659	The main functional groups of EPSs	634:667	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	2	14	theme	meaningful	297:306	arg1	Bacteriocins					235:246	Bacteriocins	235:246	Bacteriocins	235:246	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	2	14	theme	meaningful	297:306	arg1	production					278:287	exopolysaccharides (EPSs) production	252:287	exopolysaccharides (EPSs) production	252:287	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	2	14	theme	meaningful	297:306	arg1	examples					308:315	two meaningful examples	293:315	two meaningful examples of functional applications of lactobacilli	293:358	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	0	15	theme	potential	92:100	arg1	Assessment					0:9	Assessment	0:9	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	0:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	5	16	theme	functional	643:652	arg1	EPSs					664:667	EPSs	664:667	EPSs	664:667	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	5	16	theme	functional	643:652	arg1	groups					654:659	The main functional groups	634:659	The main functional groups of EPSs	634:667	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	4	17	theme	sugar	627:631	arg1	presence					584:591	the presence	580:591	the presence of glucose, galactose and unknown sugar	580:631	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	0	18	theme	Egyptian	110:117	arg1	Lactobacillus					119:131	some Egyptian Lactobacillus	105:131	some Egyptian Lactobacillus	105:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	9	19	theme	L.	1291:1292	arg1	strains					1256:1262	The selected strains	1243:1262	The selected strains	1243:1262	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	9	19	theme	L.	1291:1292	arg1	KU985436					1304:1311	L. rhamnosus KU985436	1291:1311	L. rhamnosus KU985436	1291:1311	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	6	20	theme	antioxidant	776:786	arg1	activities					788:797	powerful antioxidant activities	767:797	powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively	767:876	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	8	21	theme	activity	1220:1227	arg1	potential					1188:1196	the potential	1184:1196	the potential of Lactobacillus EPSs activity against PC-3	1184:1240	To the best of our knowledge, there have been no previous reports on the potential of Lactobacillus EPSs activity against PC-3.					
33482208	4	22	theme	monosaccharide	517:530	arg1	composition					532:542	the monosaccharide composition	513:542	the monosaccharide composition by thin layer chromatography	513:571	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	5	23	theme	EPSs	664:667	arg1	EPSs					664:667	EPSs	664:667	EPSs	664:667	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	5	23	theme	EPSs	664:667	arg1	groups					654:659	The main functional groups	634:659	The main functional groups of EPSs	634:667	The main functional groups of EPSs were elucidated by Fourier-Transform Infrared Spectroscopy.					
33482208	10	24	theme	fermented	1487:1495	arg1	milk					1497:1500	fermented milk	1487:1500	fermented milk	1487:1500	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	6	25	theme	powerful	767:774	arg1	activities					788:797	powerful antioxidant activities	767:797	powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively	767:876	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	8	26	theme	EPSs	1215:1218	arg1	activity					1220:1227	Lactobacillus EPSs activity	1201:1227	Lactobacillus EPSs activity against PC-3	1201:1240	To the best of our knowledge, there have been no previous reports on the potential of Lactobacillus EPSs activity against PC-3.					
33482208	8	27	theme	previous	1164:1171	arg1	reports					1173:1179	no previous reports	1161:1179	no previous reports on the potential of Lactobacillus EPSs activity against PC-3	1161:1240	To the best of our knowledge, there have been no previous reports on the potential of Lactobacillus EPSs activity against PC-3.					
33482208	10	28	theme	blood	1541:1545	arg1	triglyceride					1547:1558	blood triglyceride	1541:1558	blood triglyceride	1541:1558	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	10	29	theme	Westar	1470:1475	arg1	rats					1477:1480	feeding Westar rats	1462:1480	feeding Westar rats with fermented milk	1462:1500	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	2	30	theme	applications	331:342	arg1	Bacteriocins					235:246	Bacteriocins	235:246	Bacteriocins	235:246	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	2	30	theme	applications	331:342	arg1	production					278:287	exopolysaccharides (EPSs) production	252:287	exopolysaccharides (EPSs) production	252:287	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	2	30	theme	applications	331:342	arg1	examples					308:315	two meaningful examples	293:315	two meaningful examples of functional applications of lactobacilli	293:358	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	10	31	theme	LDL	1533:1535	arg1	reduction					1560:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	9	32	theme	plantarum	1268:1276	arg1	KU985433					1278:1285	L. plantarum KU985433	1265:1285	L. plantarum KU985433	1265:1285	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	9	32	theme	plantarum	1268:1276	arg1	strains					1256:1262	The selected strains	1243:1262	The selected strains	1243:1262	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	4	33	theme	glucose	596:602	arg1	presence					584:591	the presence	580:591	the presence of glucose, galactose and unknown sugar	580:631	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	7	34	theme	PC-3	1092:1095	arg1	cells					1108:1112	HCT-116, PC-3 and HepG-2 cells	1083:1112	cells	1108:1112	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	2	35	theme	functional	320:329	arg1	applications					331:342	functional applications	320:342	functional applications of lactobacilli	320:358	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	3	36	theme	Lactobacillus	388:400	arg1	strains					402:408	Eight Lactobacillus strains	382:408	Eight Lactobacillus strains	382:408	Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties.					
33482208	10	37	theme	greater	1512:1518	arg1	reduction					1560:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	0	38	dep	in	64:65	arg1	vivo					67:70	vivo	67:70	vivo	67:70	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	6	39	theme	fermentation	735:746	arg1	cultures					748:755	Their fermentation cultures	729:755	Their fermentation cultures	729:755	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	1	40	theme	low	209:211	arg1	effects					218:224	low side effects	209:224	low side effects to host	209:232	Lactobacilli probiotics have been suggested to reduce cholesterol with low side effects to host.					
33482208	0	41	theme	exopolysaccharides	14:31	arg1	Assessment					0:9	Assessment	0:9	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	0:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	4	42	theme	galactose	605:613	arg1	presence					584:591	the presence	580:591	the presence of glucose, galactose and unknown sugar	580:631	Analysis of the monosaccharide composition by thin layer chromatography showed the presence of glucose, galactose and unknown sugar.					
33482208	0	43	theme	Lactobacillus	119:131	arg1	exopolysaccharides					14:31	exopolysaccharides	14:31	exopolysaccharides	14:31	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	0	43	theme	Lactobacillus	119:131	arg1	bacteriocins					34:45	bacteriocins	34:45	bacteriocins	34:45	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	0	43	theme	Lactobacillus	119:131	arg1	potential					92:100	in vitro and in vivo hypocholesterolemic potential	51:100	in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	51:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	6	44	dep	%	953:953	arg1	to					948:949	to	948:949	to	948:949	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	8	45	from	reports	1173:1179	arg1	potential					1188:1196	the potential	1184:1196	the potential of Lactobacillus EPSs activity against PC-3	1184:1240	To the best of our knowledge, there have been no previous reports on the potential of Lactobacillus EPSs activity against PC-3.					
33482208	1	46	theme	side	213:216	arg1	effects					218:224	low side effects	209:224	low side effects to host	209:232	Lactobacilli probiotics have been suggested to reduce cholesterol with low side effects to host.					
33482208	3	47	theme	Egyptian	434:441	arg1	food					453:456	some Egyptian fermented food	429:456	some Egyptian fermented food	429:456	Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties.					
33482208	7	48	theme	IC50	1067:1070	arg1	values					1072:1077	low IC50 values	1063:1077	low IC50 values for HCT-116, PC-3 and HepG-2 cells	1063:1112	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	6	49	theme	cholesterol	902:912	arg1	downgrading					914:924	vitro cholesterol downgrading	896:924	vitro cholesterol downgrading	896:924	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	9	50	theme	L.	1265:1266	arg1	KU985433					1278:1285	L. plantarum KU985433	1265:1285	L. plantarum KU985433	1265:1285	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	9	50	theme	L.	1265:1266	arg1	strains					1256:1262	The selected strains	1243:1262	The selected strains	1243:1262	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	10	51	with	rats	1477:1480	arg1	milk					1497:1500	fermented milk	1487:1500	fermented milk	1487:1500	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	9	52	theme	different	1326:1334	arg1	bacteriocins					1336:1347	two different bacteriocins	1322:1347	two different bacteriocins	1322:1347	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	0	53	theme	bacteriocins	34:45	arg1	Assessment					0:9	Assessment	0:9	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	0:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	3	54	theme	fermented	443:451	arg1	food					453:456	some Egyptian fermented food	429:456	some Egyptian fermented food	429:456	Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties.					
33482208	6	55	theme	vitro	896:900	arg1	downgrading					914:924	vitro cholesterol downgrading	896:924	vitro cholesterol downgrading	896:924	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	0	56	dep	in	51:52	arg1	vitro					54:58	vitro	54:58	vitro	54:58	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	9	57	theme	antimicrobial	1404:1416	arg1	activities					1418:1427	good antimicrobial activities	1399:1427	good antimicrobial activities	1399:1427	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	3	58	theme	probiotic	479:487	arg1	properties					489:498	their probiotic properties	473:498	their probiotic properties	473:498	Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties.					
33482208	8	59	theme	Lactobacillus	1201:1213	arg1	activity					1220:1227	Lactobacillus EPSs activity	1201:1227	Lactobacillus EPSs activity against PC-3	1201:1240	To the best of our knowledge, there have been no previous reports on the potential of Lactobacillus EPSs activity against PC-3.					
33482208	6	60	theme	free	853:856	arg1	cells					858:862	free cells	853:862	free cells	853:862	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	9	61	theme	rhamnosus	1294:1302	arg1	strains					1256:1262	The selected strains	1243:1262	The selected strains	1243:1262	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	9	61	theme	rhamnosus	1294:1302	arg1	KU985436					1304:1311	L. rhamnosus KU985436	1291:1311	L. rhamnosus KU985436	1291:1311	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	10	62	theme	triglyceride	1547:1558	arg1	reduction					1560:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	0	63	theme	in	51:52	arg1	potential					92:100	in vitro and in vivo hypocholesterolemic potential	51:100	in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	51:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	7	64	theme	low	1063:1065	arg1	values					1072:1077	low IC50 values	1063:1077	low IC50 values for HCT-116, PC-3 and HepG-2 cells	1063:1112	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	3	65	attach	isolated	415:422	arg1	food					453:456	some Egyptian fermented food	429:456	some Egyptian fermented food	429:456	Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties.					
33482208	3	65	attach	isolated	415:422	arg2	strains					402:408	Eight Lactobacillus strains	382:408	Eight Lactobacillus strains	382:408	Eight Lactobacillus strains were isolated from some Egyptian fermented food and tested for their probiotic properties.					
33482208	7	66	theme	HepG-2	1101:1106	arg1	cells					1108:1112	HCT-116, PC-3 and HepG-2 cells	1083:1112	cells	1108:1112	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	7	67	theme	good	1007:1010	arg1	activities					1023:1032	good anticancer activities	1007:1032	good anticancer activities against carcinoma cells	1007:1056	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	9	68	theme	gel	1364:1366	arg1	chromatography					1379:1392	gel permeation chromatography	1364:1392	gel permeation chromatography with good antimicrobial activities	1364:1427	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	7	69	theme	carcinoma	1042:1050	arg1	cells					1052:1056	carcinoma cells	1042:1056	carcinoma cells	1042:1056	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	10	70	theme	In	1430:1431	arg1	study					1438:1442	In vivo study	1430:1442	In vivo study	1430:1442	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	7	71	theme	anticancer	1012:1021	arg1	activities					1023:1032	good anticancer activities	1007:1032	good anticancer activities against carcinoma cells	1007:1056	Their EPSs showed good anticancer activities against carcinoma cells with low IC50 values for HCT-116, PC-3 and HepG-2 cells.					
33482208	2	72	theme	lactobacilli	347:358	arg1	applications					331:342	functional applications	320:342	functional applications of lactobacilli	320:358	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	9	73	theme	permeation	1368:1377	arg1	chromatography					1379:1392	gel permeation chromatography	1364:1392	gel permeation chromatography with good antimicrobial activities	1364:1427	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	10	74	theme	cholesterol	1520:1530	arg1	reduction					1560:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	greater cholesterol, LDL and blood triglyceride reduction	1512:1568	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	9	75	theme	selected	1247:1254	arg1	KU985436					1304:1311	L. rhamnosus KU985436	1291:1311	L. rhamnosus KU985436	1291:1311	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	9	75	theme	selected	1247:1254	arg1	KU985433					1278:1285	L. plantarum KU985433	1265:1285	L. plantarum KU985433	1265:1285	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	9	75	theme	selected	1247:1254	arg1	strains					1256:1262	The selected strains	1243:1262	The selected strains	1243:1262	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33482208	1	76	theme	Lactobacilli	138:149	arg1	probiotics					151:160	Lactobacilli probiotics	138:160	Lactobacilli probiotics	138:160	Lactobacilli probiotics have been suggested to reduce cholesterol with low side effects to host.					
33482208	10	77	dep	In	1430:1431	arg1	vivo					1433:1436	vivo	1433:1436	vivo	1433:1436	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	0	78	theme	in	64:65	arg1	potential					92:100	in vitro and in vivo hypocholesterolemic potential	51:100	in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus	51:131	Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.					
33482208	10	79	theme	feeding	1462:1468	arg1	rats					1477:1480	feeding Westar rats	1462:1480	feeding Westar rats with fermented milk	1462:1500	In vivo study demonstrated that feeding Westar rats with fermented milk exhibited greater cholesterol, LDL and blood triglyceride reduction for both strains.					
33482208	2	80	theme	exopolysaccharides	252:269	arg1	Bacteriocins					235:246	Bacteriocins	235:246	Bacteriocins	235:246	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	2	80	theme	exopolysaccharides	252:269	arg1	production					278:287	exopolysaccharides (EPSs) production	252:287	exopolysaccharides (EPSs) production	252:287	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	2	80	theme	exopolysaccharides	252:269	arg1	examples					308:315	two meaningful examples	293:315	two meaningful examples of functional applications of lactobacilli	293:358	Bacteriocins and exopolysaccharides (EPSs) production are two meaningful examples of functional applications of lactobacilli in the food industry.					
33482208	6	81	dep	%	824:824	arg1	to					819:820	to	819:820	to	819:820	Their fermentation cultures displayed powerful antioxidant activities extending from 97.5 to 99%, 40-75% for their EPSs and free cells, respectively, and exhibited in vitro cholesterol downgrading from 48 to 82% and 72 to 91% after 48 and 120 h, respectively.					
33482208	9	82	theme	good	1399:1402	arg1	activities					1418:1427	good antimicrobial activities	1399:1427	good antimicrobial activities	1399:1427	The selected strains, L. plantarum KU985433 and L. rhamnosus KU985436 produced two different bacteriocins as detected by gel permeation chromatography with good antimicrobial activities.					
33223315	0	0	theme	PVA-SA	176:181	arg1	beads					192:196	PVA-SA hydrogel beads	176:196	PVA-SA hydrogel beads	176:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	9	1	theme	simultaneous	1340:1351	arg1	sorption					1353:1360	simultaneous sorption	1340:1360	simultaneous sorption	1340:1360	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	6	2	with	beads	940:944	arg1	bacteria					963:970	immobilized bacteria	951:970	immobilized bacteria	951:970	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	6	3	theme	hydrogel	931:938	arg1	beads					940:944	hydrogel beads	931:944	hydrogel beads with immobilized bacteria at a medium concentration	931:996	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	1	4	theme	washing	204:210	arg1	technique					259:267	a promising technique	247:267	a promising technique in removing organic pollutants from soil	247:308	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	1	4	theme	washing	204:210	arg1	process					212:218	Soil washing process	199:218	Soil washing process enhanced by surfactants	199:242	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	9	5	theme	possible	1429:1436	arg1	application					1449:1459	possible industrial application	1429:1459	possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition	1429:1539	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	2	6	theme	Triton	513:518	arg1	X-100					520:524	Triton X-100	513:524	Triton X-100 (TX-100)	513:533	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	2	6	theme	Triton	513:518	arg1	TX-100					527:532	TX-100	527:532	TX-100	527:532	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	8	7	theme	co-existing	1193:1203	arg1	substances					1205:1214	the co-existing substances	1189:1214	the co-existing substances such as TX-100	1189:1229	The bead provided protection for bacteria against the co-existing substances such as TX-100.					
33223315	8	7	theme	co-existing	1193:1203	arg1	TX-100					1224:1229	TX-100	1224:1229	TX-100	1224:1229	The bead provided protection for bacteria against the co-existing substances such as TX-100.					
33223315	9	8	with	SWSs	1511:1514	arg1	composition					1529:1539	complex composition	1521:1539	complex composition	1521:1539	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	4	9	theme	free	687:690	arg1	bacteria					692:699	free bacteria	687:699	free bacteria	687:699	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	9	10	with	deal	1501:1504	arg1	SWSs					1511:1514	SWSs	1511:1514	SWSs with complex composition	1511:1539	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	0	11	theme	immobilized	142:152	arg1	bacteria					164:171	immobilized degrading bacteria	142:171	immobilized degrading bacteria	142:171	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	4	12	from	medium	777:782	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	5	13	theme	beads	880:884	arg1	performance					856:866	The recycling and removal performance	830:866	The recycling and removal performance of the used beads	830:884	The recycling and removal performance of the used beads were also examined.					
33223315	4	14	from	low	772:774	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	0	15	theme	soil	44:47	arg1	solution					57:64	a soil washing solution	42:64	a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads	42:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	3	16	theme	pyrene-degrading	568:583	arg1	strain					595:600	a pyrene-degrading bacterial strain	566:600	a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	566:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	9	17	theme	bacteria	1464:1471	arg1	technique					1488:1496	bacteria immobilization technique	1464:1496	bacteria immobilization technique	1464:1496	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	9	18	theme	technique	1488:1496	arg1	application					1449:1459	possible industrial application	1429:1459	possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition	1429:1539	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	4	19	theme	degrading	748:756	arg1	bacteria					758:765	immobilized degrading bacteria	736:765	immobilized degrading bacteria	736:765	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	0	20	theme	simultaneous	88:99	arg1	sorption					101:108	simultaneous sorption	88:108	simultaneous sorption	88:108	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	9	21	theme	bacteria-immobilized	1236:1255	arg1	efficient					1272:1280	efficient	1272:1280	efficient	1272:1280	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	9	21	theme	bacteria-immobilized	1236:1255	arg1	beads					1257:1261	The bacteria-immobilized beads	1232:1261	The bacteria-immobilized beads	1232:1261	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	4	22	theme	PVA-SA	708:713	arg1	beads					715:719	blank PVA-SA beads	702:719	blank PVA-SA beads	702:719	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	3	23	from	strain	595:600	arg1	beads					657:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	7	24	theme	32-55	1103:1107	arg1	%					1108:1108	%	1108:1108	%	1108:1108	The beads can be recycled and reused to treat a new SWS; 32-55% ∑16PAHs was removed in 24 h.					
33223315	1	25	theme	organic	281:287	arg1	pollutants					289:298	organic pollutants	281:298	organic pollutants	281:298	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	2	26	with	plant	502:506	arg1	X-100					520:524	Triton X-100	513:524	Triton X-100 (TX-100)	513:533	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	2	26	with	plant	502:506	arg1	TX-100					527:532	TX-100	527:532	TX-100	527:532	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	9	27	theme	complex	1521:1527	arg1	composition					1529:1539	complex composition	1521:1539	complex composition	1521:1539	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	2	28	theme	soil	412:415	arg1	SWS					435:437	SWS	435:437	SWS	435:437	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	2	28	theme	soil	412:415	arg1	solution					425:432	a soil washing solution	410:432	a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100)	410:533	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	0	29	theme	PAHs	11:14	arg1	Removal					0:6	Removal	0:6	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.	0:197	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	2	30	theme	rinsing	452:458	arg1	soil					483:486	a heavily contaminated soil	460:486	rinsing a heavily contaminated soil	452:486	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	3	31	theme	alginate	630:637	arg1	beads					657:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	0	32	theme	high	19:22	arg1	concentrations					24:37	high concentrations	19:37	high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads	19:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	3	33	theme	polyvinyl	605:613	arg1	beads					657:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	4	34	theme	high	788:791	arg1	concentration					801:813	high initial concentration	788:813	high initial concentration	788:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	9	35	theme	free	1303:1306	arg1	bacteria					1308:1315	free bacteria	1303:1315	free bacteria	1303:1315	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	3	36	theme	hydrogel	648:655	arg1	beads					657:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	0	37	from	processes	129:137	arg1	beads					192:196	PVA-SA hydrogel beads	176:196	PVA-SA hydrogel beads	176:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	3	38	theme	PVA-SA	640:645	arg1	beads					657:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	0	39	contain	containing	66:75	arg1	solution					57:64	a soil washing solution	42:64	a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads	42:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	0	39	contain	containing	66:75	arg2	TX-100					77:82	TX-100	77:82	TX-100	77:82	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	4	40	with	bacteria	692:699	arg1	bacteria					758:765	immobilized degrading bacteria	736:765	immobilized degrading bacteria	736:765	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	0	41	theme	hydrogel	183:190	arg1	beads					192:196	PVA-SA hydrogel beads	176:196	PVA-SA hydrogel beads	176:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	2	42	theme	biodegradation	353:366	arg1	technique					368:376	biodegradation technique	353:376	biodegradation technique	353:376	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	1	43	theme	promising	249:257	arg1	technique					259:267	a promising technique	247:267	a promising technique in removing organic pollutants from soil	247:308	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	1	43	theme	promising	249:257	arg1	process					212:218	Soil washing process	199:218	Soil washing process enhanced by surfactants	199:242	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	0	44	attach	Removal	0:6	arg2	PAHs					11:14	PAHs	11:14	PAHs	11:14	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	0	44	attach	Removal	0:6	arg3	concentrations					24:37	high concentrations	19:37	high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads	19:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	6	45	from	∑16PAHs	1020:1026	arg1	96 h					1040:1043	96 h	1040:1043	96 h	1040:1043	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	6	46	from	SWS	1033:1035	arg1	∑16PAHs					1020:1026	∑16PAHs	1020:1026	∑16PAHs from SWS in 96 h	1020:1043	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	9	47	theme	biodegradation	1366:1379	arg1	processes					1381:1389	biodegradation processes	1366:1389	biodegradation processes	1366:1389	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	4	48	theme	initial	793:799	arg1	concentration					801:813	high initial concentration	788:813	high initial concentration	788:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	5	49	theme	recycling	834:842	arg1	performance					856:866	The recycling and removal performance	830:866	The recycling and removal performance of the used beads	830:884	The recycling and removal performance of the used beads were also examined.					
33223315	0	50	theme	biodegradation	114:127	arg1	processes					129:137	biodegradation processes	114:137	biodegradation processes	114:137	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	1	51	theme	Soil	199:202	arg1	technique					259:267	a promising technique	247:267	a promising technique in removing organic pollutants from soil	247:308	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	1	51	theme	Soil	199:202	arg1	process					212:218	Soil washing process	199:218	Soil washing process enhanced by surfactants	199:242	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	4	52	with	beads	725:729	arg1	bacteria					758:765	immobilized degrading bacteria	736:765	immobilized degrading bacteria	736:765	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	9	53	theme	solid	1409:1413	arg1	reference					1415:1423	a solid reference	1407:1423	a solid reference	1407:1423	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	4	54	with	beads	715:719	arg1	bacteria					758:765	immobilized degrading bacteria	736:765	immobilized degrading bacteria	736:765	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	0	55	theme	degrading	154:162	arg1	bacteria					164:171	immobilized degrading bacteria	142:171	immobilized degrading bacteria	142:171	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	5	56	theme	used	875:878	arg1	beads					880:884	the used beads	871:884	the used beads	871:884	The recycling and removal performance of the used beads were also examined.					
33223315	0	57	from	sorption	101:108	arg1	beads					192:196	PVA-SA hydrogel beads	176:196	PVA-SA hydrogel beads	176:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	3	58	theme	bacterial	585:593	arg1	strain					595:600	a pyrene-degrading bacterial strain	566:600	a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	566:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	9	59	theme	industrial	1438:1447	arg1	application					1449:1459	possible industrial application	1429:1459	possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition	1429:1539	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	2	60	used	used	382:385	arg2	technique					368:376	biodegradation technique	353:376	biodegradation technique	353:376	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	2	60	used	used	382:385	arg2	sorption					340:347	a simultaneous sorption	325:347	a simultaneous sorption	325:347	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	4	61	theme	bacteria	692:699	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	0	62	theme	washing	49:55	arg1	solution					57:64	a soil washing solution	42:64	a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads	42:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	1	63	attach	removing	272:279	arg2	process					212:218	Soil washing process	199:218	Soil washing process enhanced by surfactants	199:242	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	1	63	attach	removing	272:279	arg2	technique					259:267	a promising technique	247:267	a promising technique in removing organic pollutants from soil	247:308	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	1	63	attach	removing	272:279	arg1	soil					305:308	soil	305:308	soil	305:308	Soil washing process enhanced by surfactants is a promising technique in removing organic pollutants from soil.					
33223315	5	64	theme	removal	848:854	arg1	performance					856:866	The recycling and removal performance	830:866	The recycling and removal performance of the used beads	830:884	The recycling and removal performance of the used beads were also examined.					
33223315	6	65	theme	medium	977:982	arg1	concentration					984:996	a medium concentration	975:996	a medium concentration	975:996	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	2	66	theme	contaminated	470:481	arg1	soil					483:486	a heavily contaminated soil	460:486	rinsing a heavily contaminated soil	452:486	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	9	67	theme	immobilization	1473:1486	arg1	technique					1488:1496	bacteria immobilization technique	1464:1496	bacteria immobilization technique	1464:1496	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	7	68	dep	SWS	1098:1100	arg1	removed					1122:1128	removed	1122:1128	was removed in 24 h	1118:1136	The beads can be recycled and reused to treat a new SWS; 32-55% ∑16PAHs was removed in 24 h.					
33223315	4	69	theme	Removal	664:670	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	6	70	theme	immobilized	951:961	arg1	bacteria					963:970	immobilized bacteria	951:970	immobilized bacteria	951:970	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	2	71	theme	coking	495:500	arg1	plant					502:506	a coking plant	493:506	a coking plant with Triton X-100 (TX-100)	493:533	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	6	72	from	concentration	984:996	arg1	beads					940:944	hydrogel beads	931:944	hydrogel beads with immobilized bacteria at a medium concentration	931:996	Our findings showed that hydrogel beads with immobilized bacteria at a medium concentration can remove around 77% ∑16PAHs from SWS in 96 h.					
33223315	4	73	theme	beads	725:729	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	7	74	theme	%	1108:1108	arg1	∑16PAHs					1110:1116	32-55% ∑16PAHs	1103:1116	32-55% ∑16PAHs	1103:1116	The beads can be recycled and reused to treat a new SWS; 32-55% ∑16PAHs was removed in 24 h.					
33223315	0	75	from	concentrations	24:37	arg1	solution					57:64	a soil washing solution	42:64	a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads	42:196	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	0	75	from	concentrations	24:37	arg1	Removal					0:6	Removal	0:6	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.	0:197	Removal of PAHs at high concentrations in a soil washing solution containing TX-100 via simultaneous sorption and biodegradation processes by immobilized degrading bacteria in PVA-SA hydrogel beads.					
33223315	4	76	theme	immobilized	736:746	arg1	bacteria					758:765	immobilized degrading bacteria	736:765	immobilized degrading bacteria	736:765	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	4	77	theme	blank	702:706	arg1	beads					715:719	blank PVA-SA beads	702:719	blank PVA-SA beads	702:719	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	7	78	theme	new	1094:1096	arg1	SWS					1098:1100	a new SWS	1092:1100	a new SWS; 32-55% ∑16PAHs was removed in 24 h	1092:1136	The beads can be recycled and reused to treat a new SWS; 32-55% ∑16PAHs was removed in 24 h.					
33223315	2	79	theme	washing	417:423	arg1	SWS					435:437	SWS	435:437	SWS	435:437	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	2	79	theme	washing	417:423	arg1	solution					425:432	a soil washing solution	410:432	a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100)	410:533	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	3	80	theme	alcohol-sodium	615:628	arg1	beads					657:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads	605:661	This was done by immobilizing a pyrene-degrading bacterial strain in polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel beads.					
33223315	4	81	theme	beads	715:719	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	4	82	from	concentration	801:813	arg1	performance					672:682	Removal performance	664:682	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration	664:813	Removal performance of free bacteria, blank PVA-SA beads and beads with immobilized degrading bacteria at a low, medium and high initial concentration was evaluated.					
33223315	2	83	theme	simultaneous	327:338	arg1	sorption					340:347	a simultaneous sorption	325:347	a simultaneous sorption	325:347	In this work, a simultaneous sorption and biodegradation technique was used to remove 16 PAHs from a soil washing solution (SWS) obtained by rinsing a heavily contaminated soil from a coking plant with Triton X-100 (TX-100).					
33223315	9	84	theme	blank	1321:1325	arg1	beads					1327:1331	blank beads	1321:1331	blank beads	1321:1331	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
33223315	9	85	theme	due	1333:1335	arg1	bacteria					1308:1315	free bacteria	1303:1315	free bacteria	1303:1315	The bacteria-immobilized beads are more efficient and sustainable than free bacteria and blank beads due to simultaneous sorption and biodegradation processes, thus providing a solid reference for possible industrial application of bacteria immobilization technique to deal with SWSs with complex composition.					
32084311	6	0	theme	hydrogel	924:931	arg1	system					933:938	this hydrogel system	919:938	this hydrogel system	919:938	Furthermore, this hydrogel system can support cell proliferation, migration, and function.					
32084311	4	1	theme	composite	740:748	arg1	hydrogel					750:757	the developed composite hydrogel	726:757	the developed composite hydrogel	726:757	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	2	2	theme	tunable	367:373	arg1	structure					375:383	tunable structure	367:383	tunable structure	367:383	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	7	3	theme	tissue	1128:1133	arg1	engineering					1135:1145	tissue engineering	1128:1145	tissue engineering	1128:1145	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	0	4	theme	Cell	100:103	arg1	Culture					105:111	Cell Culture	100:111	Cell Culture	100:111	Biocompatible Hydrogels Based on Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture.					
32084311	5	5	theme	hydrogel	896:903	arg1	non-toxicity					868:879	the non-toxicity	864:879	the non-toxicity of this hybrid hydrogel	864:903	Importantly, cytotoxicity assays show the non-toxicity of this hybrid hydrogel.					
32084311	4	6	theme	developed	730:738	arg1	hydrogel					750:757	the developed composite hydrogel	726:757	the developed composite hydrogel	726:757	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	7	7	theme	function	1206:1213	arg1	terms					1150:1154	terms	1150:1154	terms of cell engraftment, survival, proliferation, and function	1150:1213	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	4	8	theme	hydrogel	750:757	arg1	strength					668:675	mechanical strength	657:675	mechanical strength	657:675	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	8	theme	hydrogel	750:757	arg1	swelling					697:704	swelling	697:704	swelling	697:704	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	8	theme	hydrogel	750:757	arg1	morphology					711:720	morphology	711:720	morphology	711:720	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	8	theme	hydrogel	750:757	arg1	properties					637:646	The physicochemical properties	617:646	The physicochemical properties	617:646	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	8	theme	hydrogel	750:757	arg1	stability					686:694	thermal stability	678:694	thermal stability	678:694	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	2	9	theme	mechanical	339:348	arg1	performance					350:360	sufficient mechanical performance	328:360	sufficient mechanical performance	328:360	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	7	10	theme	proliferation	1187:1199	arg1	terms					1150:1154	terms	1150:1154	terms of cell engraftment, survival, proliferation, and function	1150:1213	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	5	11	theme	cytotoxicity	839:850	arg1	assays					852:857	cytotoxicity assays	839:857	cytotoxicity assays	839:857	Importantly, cytotoxicity assays show the non-toxicity of this hybrid hydrogel.					
32084311	2	12	theme	clinical	279:286	arg1	utilization					288:298	clinical utilization	279:298	clinical utilization	279:298	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	4	13	theme	mechanical	657:666	arg1	strength					668:675	mechanical strength	657:675	mechanical strength	657:675	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	1	14	theme	future	172:177	arg1	delivery					221:228	targeted delivery	212:228	targeted delivery	212:228	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	1	14	theme	future	172:177	arg1	applications					190:201	future biomedical applications	172:201	future biomedical applications	172:201	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	1	14	theme	future	172:177	arg1	engineering					241:251	tissue engineering	234:251	tissue engineering	234:251	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	7	15	theme	survival	1177:1184	arg1	terms					1150:1154	terms	1150:1154	terms of cell engraftment, survival, proliferation, and function	1150:1213	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	2	16	theme	sufficient	328:337	arg1	performance					350:360	sufficient mechanical performance	328:360	sufficient mechanical performance	328:360	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	1	17	theme	biomedical	179:188	arg1	delivery					221:228	targeted delivery	212:228	targeted delivery	212:228	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	1	17	theme	biomedical	179:188	arg1	applications					190:201	future biomedical applications	172:201	future biomedical applications	172:201	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	1	17	theme	biomedical	179:188	arg1	engineering					241:251	tissue engineering	234:251	tissue engineering	234:251	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	0	18	theme	Biocompatible	0:12	arg1	Hydrogels					14:22	Biocompatible Hydrogels	0:22	Biocompatible Hydrogels	0:22	Biocompatible Hydrogels Based on Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture.					
32084311	7	19	dep	cell	1159:1162	arg1	engraftment					1164:1174	engraftment	1164:1174	engraftment	1164:1174	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	2	20	theme	hydrogels	403:411	arg1	utilization					288:298	clinical utilization	279:298	clinical utilization	279:298	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	2	20	theme	hydrogels	403:411	arg1	biocompatibility					310:325	reliable biocompatibility	301:325	reliable biocompatibility	301:325	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	2	20	theme	hydrogels	403:411	arg1	performance					350:360	sufficient mechanical performance	328:360	sufficient mechanical performance	328:360	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	2	20	theme	hydrogels	403:411	arg1	structure					375:383	tunable structure	367:383	tunable structure	367:383	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	7	21	theme	hydrogel	1080:1087	arg1	scaffold					1089:1096	a polysaccharide-constructed hydrogel scaffold	1051:1096	a polysaccharide-constructed hydrogel scaffold	1051:1096	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	3	22	theme	thickening	557:566	arg1	agent					568:572	a thickening agent	555:572	a thickening agent	555:572	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	3	22	theme	thickening	557:566	arg1	former					545:550	former	545:550	former	545:550	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	3	23	theme	polysaccharide	475:488	arg1	hydrogel					490:497	a unique hybrid polysaccharide hydrogel	459:497	a unique hybrid polysaccharide hydrogel	459:497	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	2	24	theme	reliable	301:308	arg1	biocompatibility					310:325	reliable biocompatibility	301:325	reliable biocompatibility	301:325	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	4	25	theme	physicochemical	621:635	arg1	strength					668:675	mechanical strength	657:675	mechanical strength	657:675	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	25	theme	physicochemical	621:635	arg1	swelling					697:704	swelling	697:704	swelling	697:704	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	25	theme	physicochemical	621:635	arg1	morphology					711:720	morphology	711:720	morphology	711:720	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	25	theme	physicochemical	621:635	arg1	properties					637:646	The physicochemical properties	617:646	The physicochemical properties	617:646	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	4	25	theme	physicochemical	621:635	arg1	stability					686:694	thermal stability	678:694	thermal stability	678:694	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	3	26	theme	unique	461:466	arg1	hydrogel					490:497	a unique hybrid polysaccharide hydrogel	459:497	a unique hybrid polysaccharide hydrogel	459:497	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	0	27	theme	Food	33:36	arg1	Gums					38:41	Food Gums	33:41	Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture	33:111	Biocompatible Hydrogels Based on Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture.					
32084311	7	28	theme	cell	1159:1162	arg1	terms					1150:1154	terms	1150:1154	terms of cell engraftment, survival, proliferation, and function	1150:1213	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	4	29	theme	polysaccharide	802:815	arg1	content					817:823	the polysaccharide content	798:823	the polysaccharide content	798:823	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	1	30	theme	targeted	212:219	arg1	delivery					221:228	targeted delivery	212:228	targeted delivery	212:228	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	0	31	theme	Tunable	48:54	arg1	Properties					72:81	Tunable Physicochemical Properties	48:81	Tunable Physicochemical Properties as Scaffolds for Cell Culture	48:111	Biocompatible Hydrogels Based on Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture.					
32084311	5	32	theme	hybrid	889:894	arg1	hydrogel					896:903	this hybrid hydrogel	884:903	this hybrid hydrogel	884:903	Importantly, cytotoxicity assays show the non-toxicity of this hybrid hydrogel.					
32084311	7	33	theme	polysaccharide-constructed	1053:1078	arg1	scaffold					1089:1096	a polysaccharide-constructed hydrogel scaffold	1051:1096	a polysaccharide-constructed hydrogel scaffold	1051:1096	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	7	34	theme	new	1029:1031	arg1	strategy					1033:1040	a new strategy	1027:1040	a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function	1027:1213	Altogether, our work proposes a new strategy to build a polysaccharide-constructed hydrogel scaffold, which holds much promise for tissue engineering in terms of cell engraftment, survival, proliferation, and function.					
32084311	3	35	from	agent	568:572	arg1	hydrogel					490:497	a unique hybrid polysaccharide hydrogel	459:497	a unique hybrid polysaccharide hydrogel	459:497	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	1	36	theme	food	136:139	arg1	gums					141:144	food gums	136:144	food gums	136:144	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	3	37	theme	hybrid	468:473	arg1	hydrogel					490:497	a unique hybrid polysaccharide hydrogel	459:497	a unique hybrid polysaccharide hydrogel	459:497	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	3	38	theme	network	601:607	arg1	matrix					609:614	a network matrix	599:614	a network matrix	599:614	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	3	38	theme	network	601:607	arg1	latter					582:587	latter	582:587	latter	582:587	In this work, we report a unique hybrid polysaccharide hydrogel composed of salecan and curdlan, in which the former is a thickening agent and the latter serves as a network matrix.					
32084311	2	39	theme	polysaccharide	388:401	arg1	hydrogels					403:411	polysaccharide hydrogels	388:411	polysaccharide hydrogels	388:411	For their translation to clinical utilization, reliable biocompatibility, sufficient mechanical performance, and tunable structure of polysaccharide hydrogels are required aspects.					
32084311	1	40	theme	tissue	234:239	arg1	engineering					241:251	tissue engineering	234:251	tissue engineering	234:251	Hydrogels composed of food gums have gained attention for future biomedical applications, such as targeted delivery and tissue engineering.					
32084311	0	41	theme	Physicochemical	56:70	arg1	Properties					72:81	Tunable Physicochemical Properties	48:81	Tunable Physicochemical Properties as Scaffolds for Cell Culture	48:111	Biocompatible Hydrogels Based on Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture.					
32084311	0	42	with	Gums	38:41	arg1	Properties					72:81	Tunable Physicochemical Properties	48:81	Tunable Physicochemical Properties as Scaffolds for Cell Culture	48:111	Biocompatible Hydrogels Based on Food Gums with Tunable Physicochemical Properties as Scaffolds for Cell Culture.					
32084311	4	43	theme	thermal	678:684	arg1	stability					686:694	thermal stability	678:694	thermal stability	678:694	The physicochemical properties, such as mechanical strength, thermal stability, swelling, and morphology, of the developed composite hydrogel can be accurately modulated by varying the polysaccharide content.					
32084311	6	44	theme	cell	952:955	arg1	proliferation					957:969	cell proliferation	952:969	cell proliferation	952:969	Furthermore, this hydrogel system can support cell proliferation, migration, and function.					
32414454	4	0	with	content	734:740	arg1	dense					707:711	dense	707:711	dense	707:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	4	0	with	content	734:740	arg1	network					685:691	a fiber network	677:691	a fiber network that was more dense	677:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	6	1	from	potential	1019:1027	arg1	application					1032:1042	application	1032:1042	application	1032:1042	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	5	2	theme	looser	808:813	arg1	network					815:821	a looser network	806:821	a looser network with the smoothest luminal surface and greater hemocompatibility	806:886	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	4	3	theme	fiber	679:683	arg1	dense					707:711	dense	707:711	dense	707:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	4	3	theme	fiber	679:683	arg1	network					685:691	a fiber network	677:691	a fiber network that was more dense	677:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	0	4	theme	Conduits	90:97	arg1	Biocompatibility					40:55	In Vitro Biocompatibility	31:55	In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications	31:127	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	0	4	theme	Conduits	90:97	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties	0:25	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	1	5	theme	small	261:265	arg1	D-BNC					332:336	D-BNC	332:336	D-BNC	332:336	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	5	theme	small	261:265	arg1	S-BNC					322:326	S-BNC	322:326	S-BNC	322:326	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	5	theme	small	261:265	arg1	G-BNC					315:319	G-BNC	315:319	G-BNC	315:319	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	5	theme	small	261:265	arg1	conduits					305:312	three small caliber bacterial nanocellulose (BNC) conduits	255:312	three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC)	255:337	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	0	6	theme	Vessel	109:114	arg1	Applications					116:127	Blood Vessel Applications	103:127	Blood Vessel Applications	103:127	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	6	7	theme	comprehensive	899:911	arg1	evaluation					913:922	comprehensive evaluation	899:922	comprehensive evaluation of mechanical properties and performance	899:963	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	8	theme	properties	938:947	arg1	evaluation					913:922	comprehensive evaluation	899:922	comprehensive evaluation of mechanical properties and performance	899:963	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	3	9	theme	weakest	502:508	arg1	properties					521:530	weakest mechanical properties	502:530	weakest mechanical properties	502:530	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	6	10	theme	small	1047:1051	arg1	potential					1019:1027	greater potential	1011:1027	greater potential in application	1011:1042	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	10	theme	small	1047:1051	arg1	grafts					1070:1075	small caliber vascular grafts	1047:1075	small caliber vascular grafts	1047:1075	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	1	11	theme	caliber	267:273	arg1	D-BNC					332:336	D-BNC	332:336	D-BNC	332:336	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	11	theme	caliber	267:273	arg1	S-BNC					322:326	S-BNC	322:326	S-BNC	322:326	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	11	theme	caliber	267:273	arg1	G-BNC					315:319	G-BNC	315:319	G-BNC	315:319	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	11	theme	caliber	267:273	arg1	conduits					305:312	three small caliber bacterial nanocellulose (BNC) conduits	255:312	three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC)	255:337	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	0	12	theme	Blood	103:107	arg1	Applications					116:127	Blood Vessel Applications	103:127	Blood Vessel Applications	103:127	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	4	13	theme	BNC	730:732	arg1	content					734:740	the greatest BNC content	717:740	the greatest BNC content	717:740	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	1	14	theme	bacterial	275:283	arg1	D-BNC					332:336	D-BNC	332:336	D-BNC	332:336	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	14	theme	bacterial	275:283	arg1	S-BNC					322:326	S-BNC	322:326	S-BNC	322:326	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	14	theme	bacterial	275:283	arg1	G-BNC					315:319	G-BNC	315:319	G-BNC	315:319	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	14	theme	bacterial	275:283	arg1	conduits					305:312	three small caliber bacterial nanocellulose (BNC) conduits	255:312	three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC)	255:337	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	6	15	theme	caliber	1053:1059	arg1	potential					1019:1027	greater potential	1011:1027	greater potential in application	1011:1042	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	15	theme	caliber	1053:1059	arg1	grafts					1070:1075	small caliber vascular grafts	1047:1075	small caliber vascular grafts	1047:1075	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	2	16	theme	clinically-used	381:395	arg1	graft					403:407	a clinically-used ePTFE graft	379:407	a clinically-used ePTFE graft	379:407	They were compared systematically with a clinically-used ePTFE graft.					
32414454	1	17	dep	conduits	305:312	arg1	D-BNC					332:336	D-BNC	332:336	D-BNC	332:336	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	17	dep	conduits	305:312	arg1	S-BNC					322:326	S-BNC	322:326	S-BNC	322:326	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	17	dep	conduits	305:312	arg1	G-BNC					315:319	G-BNC	315:319	G-BNC	315:319	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	17	dep	conduits	305:312	arg1	conduits					305:312	three small caliber bacterial nanocellulose (BNC) conduits	255:312	three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC)	255:337	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	6	18	theme	conduits	1115:1122	arg1	patency					1090:1096	the patency	1086:1096	the patency of the three BNC conduits	1086:1122	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	1	19	from	design	138:143	arg1	combination					169:179	combination	169:179	combination with two previously reported designs of bioreactor	169:230	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	5	20	theme	luminal	842:848	arg1	surface					850:856	the smoothest luminal surface	828:856	the smoothest luminal surface	828:856	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	4	21	with	structure	662:670	arg1	dense					707:711	dense	707:711	dense	707:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	4	21	with	structure	662:670	arg1	network					685:691	a fiber network	677:691	a fiber network that was more dense	677:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	6	22	theme	animal	1152:1157	arg1	studies					1159:1165	animal studies	1152:1165	animal studies	1152:1165	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	4	23	theme	unstratified	649:660	arg1	structure					662:670	an unstratified structure	646:670	an unstratified structure with a fiber network that was more dense	646:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	0	24	theme	Physicochemical	0:14	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties	0:25	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	1	25	theme	nanocellulose	285:297	arg1	D-BNC					332:336	D-BNC	332:336	D-BNC	332:336	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	25	theme	nanocellulose	285:297	arg1	S-BNC					322:326	S-BNC	322:326	S-BNC	322:326	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	25	theme	nanocellulose	285:297	arg1	G-BNC					315:319	G-BNC	315:319	G-BNC	315:319	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	25	theme	nanocellulose	285:297	arg1	conduits					305:312	three small caliber bacterial nanocellulose (BNC) conduits	255:312	three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC)	255:337	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	4	26	contain	possessed	636:644	arg2	structure					662:670	an unstratified structure	646:670	an unstratified structure with a fiber network that was more dense	646:711	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	4	26	contain	possessed	636:644	arg2	content					734:740	the greatest BNC content	717:740	the greatest BNC content	717:740	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	4	26	contain	possessed	636:644	arg1	conduit					628:634	The D-BNC conduit	618:634	The D-BNC conduit	618:634	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	5	27	theme	smoothest	832:840	arg1	surface					850:856	the smoothest luminal surface	828:856	the smoothest luminal surface	828:856	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	3	28	theme	blood	598:602	arg1	vessel					604:609	an artificial blood vessel	584:609	an artificial blood vessel alone	584:615	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	0	29	theme	In	31:32	arg1	Biocompatibility					40:55	In Vitro Biocompatibility	31:55	In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications	31:127	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	6	30	dep	grafts	1070:1075	arg1	need					1124:1127	need	1124:1127	need be further verified in animal studies in vivo	1124:1173	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	4	31	theme	greatest	721:728	arg1	content					734:740	the greatest BNC content	717:740	the greatest BNC content	717:740	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	1	32	theme	reported	201:208	arg1	designs					210:216	two previously reported designs	186:216	two previously reported designs of bioreactor	186:230	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	6	33	theme	BNC	1111:1113	arg1	conduits					1115:1122	the three BNC conduits	1101:1122	the three BNC conduits	1101:1122	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	1	34	theme	BNC	300:302	arg1	D-BNC					332:336	D-BNC	332:336	D-BNC	332:336	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	34	theme	BNC	300:302	arg1	S-BNC					322:326	S-BNC	322:326	S-BNC	322:326	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	34	theme	BNC	300:302	arg1	G-BNC					315:319	G-BNC	315:319	G-BNC	315:319	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	1	34	theme	BNC	300:302	arg1	conduits					305:312	three small caliber bacterial nanocellulose (BNC) conduits	255:312	three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC)	255:337	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	6	35	contain	possess	1003:1009	arg2	grafts					1070:1075	small caliber vascular grafts	1047:1075	small caliber vascular grafts	1047:1075	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	35	contain	possess	1003:1009	arg2	potential					1019:1027	greater potential	1011:1027	greater potential in application	1011:1042	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	35	contain	possess	1003:1009	arg1	G-BNC					990:994	G-BNC	990:994	G-BNC	990:994	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	35	contain	possess	1003:1009	arg1	D-BNC					980:984	D-BNC	980:984	D-BNC	980:984	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	3	36	theme	BNC	460:462	arg1	content					464:470	the lowest BNC content	449:470	the lowest BNC content	449:470	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	3	37	theme	luminal	482:488	arg1	surface					490:496	roughest luminal surface	473:496	roughest luminal surface	473:496	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	5	38	contain	possessed	796:804	arg1	G-BNC					790:794	G-BNC	790:794	G-BNC	790:794	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	5	38	contain	possessed	796:804	arg2	network					815:821	a looser network	806:821	a looser network with the smoothest luminal surface and greater hemocompatibility	806:886	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	4	39	theme	D-BNC	622:626	arg1	conduit					628:634	The D-BNC conduit	618:634	The D-BNC conduit	618:634	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	3	40	theme	laminated	428:436	arg1	structure					438:446	a laminated structure	426:446	a laminated structure	426:446	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	5	41	with	network	815:821	arg1	surface					850:856	the smoothest luminal surface	828:856	the smoothest luminal surface	828:856	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	5	41	with	network	815:821	arg1	hemocompatibility					870:886	greater hemocompatibility	862:886	greater hemocompatibility	862:886	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	6	42	theme	greater	1011:1017	arg1	potential					1019:1027	greater potential	1011:1027	greater potential in application	1011:1042	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	42	theme	greater	1011:1017	arg1	grafts					1070:1075	small caliber vascular grafts	1047:1075	small caliber vascular grafts	1047:1075	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	1	43	theme	bioreactor	221:230	arg1	designs					210:216	two previously reported designs	186:216	two previously reported designs of bioreactor	186:230	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	5	44	theme	greater	862:868	arg1	hemocompatibility					870:886	greater hemocompatibility	862:886	greater hemocompatibility	862:886	G-BNC possessed a looser network with the smoothest luminal surface and greater hemocompatibility.					
32414454	3	45	theme	roughest	473:480	arg1	surface					490:496	roughest luminal surface	473:496	roughest luminal surface	473:496	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	1	46	theme	novel	132:136	arg1	design					138:143	A novel design	130:143	A novel design	130:143	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	4	47	theme	mechanical	767:776	arg1	properties					778:787	the strongest mechanical properties	753:787	the strongest mechanical properties	753:787	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	1	48	with	combination	169:179	arg1	designs					210:216	two previously reported designs	186:216	two previously reported designs of bioreactor	186:230	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	3	49	contain	possessed	416:424	arg2	properties					521:530	weakest mechanical properties	502:530	weakest mechanical properties	502:530	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	3	49	contain	possessed	416:424	arg2	content					464:470	the lowest BNC content	449:470	the lowest BNC content	449:470	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	3	49	contain	possessed	416:424	arg2	structure					438:446	a laminated structure	426:446	a laminated structure	426:446	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	3	49	contain	possessed	416:424	arg2	surface					490:496	roughest luminal surface	473:496	roughest luminal surface	473:496	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	3	49	contain	possessed	416:424	arg1	S-BNC					410:414	S-BNC	410:414	S-BNC	410:414	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	0	50	dep	In	31:32	arg1	Vitro					34:38	Vitro	34:38	Vitro	34:38	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	3	51	dep	and	533:535	arg1	so					537:538	so	537:538	so	537:538	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	1	52	used	used	237:240	arg2	design					138:143	A novel design	130:143	A novel design	130:143	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	6	53	theme	vascular	1061:1068	arg1	potential					1019:1027	greater potential	1011:1027	greater potential in application	1011:1042	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	6	53	theme	vascular	1061:1068	arg1	grafts					1070:1075	small caliber vascular grafts	1047:1075	small caliber vascular grafts	1047:1075	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	3	54	theme	mechanical	510:519	arg1	properties					521:530	weakest mechanical properties	502:530	weakest mechanical properties	502:530	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	4	55	theme	strongest	757:765	arg1	properties					778:787	the strongest mechanical properties	753:787	the strongest mechanical properties	753:787	The D-BNC conduit possessed an unstratified structure with a fiber network that was more dense and the greatest BNC content, providing the strongest mechanical properties.					
32414454	0	56	theme	Nanocellulose	76:88	arg1	Conduits					90:97	Three Bacterial Nanocellulose Conduits	60:97	Three Bacterial Nanocellulose Conduits	60:97	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	2	57	theme	ePTFE	397:401	arg1	graft					403:407	a clinically-used ePTFE graft	379:407	a clinically-used ePTFE graft	379:407	They were compared systematically with a clinically-used ePTFE graft.					
32414454	3	58	theme	lowest	453:458	arg1	content					464:470	the lowest BNC content	449:470	the lowest BNC content	449:470	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	1	59	theme	bioreactor	148:157	arg1	G-BNC					159:163	bioreactor G-BNC	148:163	bioreactor G-BNC	148:163	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	0	60	theme	Bacterial	66:74	arg1	Conduits					90:97	Three Bacterial Nanocellulose Conduits	60:97	Three Bacterial Nanocellulose Conduits	60:97	Physicochemical Properties and In Vitro Biocompatibility of Three Bacterial Nanocellulose Conduits for Blood Vessel Applications.					
32414454	6	61	theme	mechanical	927:936	arg1	properties					938:947	mechanical properties	927:947	mechanical properties	927:947	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32414454	3	62	theme	artificial	587:596	arg1	vessel					604:609	an artificial blood vessel	584:609	an artificial blood vessel alone	584:615	S-BNC possessed a laminated structure, the lowest BNC content, roughest luminal surface and weakest mechanical properties, and so might not be sufficiently strong for use as an artificial blood vessel alone.					
32414454	1	63	theme	G-BNC	159:163	arg1	design					138:143	A novel design	130:143	A novel design	130:143	A novel design of bioreactor G-BNC, in combination with two previously reported designs of bioreactor were used to fabricate three small caliber bacterial nanocellulose (BNC) conduits (G-BNC, S-BNC and D-BNC).					
32414454	6	64	theme	performance	953:963	arg1	evaluation					913:922	comprehensive evaluation	899:922	comprehensive evaluation of mechanical properties and performance	899:963	Following comprehensive evaluation of mechanical properties and performance, we judge that D-BNC and G-BNC should possess greater potential in application as small caliber vascular grafts, however the patency of the three BNC conduits need be further verified in animal studies in vivo.					
32815257	5	0	theme	cells	666:670	arg1	encapsulation					630:642	In situ encapsulation	622:642	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells)	622:685	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	3	1	theme	hydrogel	529:536	arg1	composite					538:546	hydrogel composite	529:546	hydrogel composite (AEC)	529:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	3	1	theme	hydrogel	529:536	arg1	AEC					549:551	AEC	549:551	AEC	549:551	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	6	2	from	increase	940:947	arg1	growth					957:962	cell growth	952:962	cell growth	952:962	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	6	3	theme	sevenfold	930:938	arg1	increase					940:947	sevenfold increase	930:947	sevenfold increase in cell growth over 1 week of culture	930:985	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	6	4	from	response	1033:1040	arg1	growth					957:962	cell growth	952:962	cell growth	952:962	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	6	5	theme	enhanced	829:836	arg1	interaction					850:860	the enhanced cell-matrix interaction	825:860	the enhanced cell-matrix interaction	825:860	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	6	6	theme	insulin	1025:1031	arg1	response					1033:1040	characteristic glucose-stimulated insulin response	991:1040	characteristic glucose-stimulated insulin response	991:1040	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	5	7	theme	MIN6	673:676	arg1	cells					666:670	pancreatic β islet cells	647:670	pancreatic β islet cells (MIN6 β-cells)	647:685	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	7	theme	MIN6	673:676	arg1	β-cells					678:684	MIN6 β-cells	673:684	MIN6 β-cells	673:684	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	8	theme	In	622:623	arg1	encapsulation					630:642	In situ encapsulation	622:642	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells)	622:685	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	2	9	theme	viability	318:326	arg1	loss					304:307	the peri-transplantation loss	279:307	the peri-transplantation loss of islet viability and functionality in the microcapsules	279:365	However, its clinical translation is hampered by the peri-transplantation loss of islet viability and functionality in the microcapsules.					
32815257	6	10	theme	glucose-stimulated	1006:1023	arg1	response					1033:1040	characteristic glucose-stimulated insulin response	991:1040	characteristic glucose-stimulated insulin response	991:1040	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	3	11	theme	novel	384:388	arg1	material					430:437	a novel islet cells biomimetic microencapsulant material	382:437	a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC)	382:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	2	12	theme	islet	312:316	arg1	viability					318:326	islet viability	312:326	islet viability	312:326	However, its clinical translation is hampered by the peri-transplantation loss of islet viability and functionality in the microcapsules.					
32815257	4	13	theme	human	602:606	arg1	lipoaspirate					608:619	human lipoaspirate	602:619	human lipoaspirate	602:619	The ECM component is derived from human lipoaspirate.					
32815257	5	14	theme	matrix	743:748	arg1	gelation					770:777	thermal-induced gelation	754:777	thermal-induced gelation of the pepsin-solubilized ECM pre-gel	754:815	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	14	theme	matrix	743:748	arg1	gelation					718:725	ionotropic gelation	707:725	ionotropic gelation of the alginate matrix	707:748	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	3	15	theme	interpenetrating	460:475	arg1	networks					477:484	the interpenetrating networks	456:484	the interpenetrating networks of alginate	456:496	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	6	16	theme	characteristic	991:1004	arg1	response					1033:1040	characteristic glucose-stimulated insulin response	991:1040	characteristic glucose-stimulated insulin response	991:1040	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	5	17	theme	pepsin-solubilized	786:803	arg1	pre-gel					809:815	the pepsin-solubilized ECM pre-gel	782:815	the pepsin-solubilized ECM pre-gel	782:815	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	3	18	theme	microencapsulant	413:428	arg1	material					430:437	a novel islet cells biomimetic microencapsulant material	382:437	a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC)	382:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	1	19	theme	type	204:207	arg1	mellitus					220:227	type 1 diabetes mellitus	204:227	type 1 diabetes mellitus	204:227	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	3	20	theme	biomimetic	402:411	arg1	material					430:437	a novel islet cells biomimetic microencapsulant material	382:437	a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC)	382:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	5	21	theme	β	658:658	arg1	cells					666:670	pancreatic β islet cells	647:670	pancreatic β islet cells (MIN6 β-cells)	647:685	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	21	theme	β	658:658	arg1	β-cells					678:684	MIN6 β-cells	673:684	MIN6 β-cells	673:684	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	22	theme	thermal-induced	754:768	arg1	gelation					770:777	thermal-induced gelation	754:777	thermal-induced gelation of the pepsin-solubilized ECM pre-gel	754:815	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	0	23	dep	Encapsulation	97:109	arg1	Network					17:23	Interpenetrating Network	0:23	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells	0:95	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	0	24	theme	Interpenetrating	0:15	arg1	Network					17:23	Interpenetrating Network	0:23	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells	0:95	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	4	25	theme	ECM	572:574	arg1	component					576:584	The ECM component	568:584	The ECM component	568:584	The ECM component is derived from human lipoaspirate.					
32815257	5	26	theme	islet	660:664	arg1	cells					666:670	pancreatic β islet cells	647:670	pancreatic β islet cells (MIN6 β-cells)	647:685	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	26	theme	islet	660:664	arg1	β-cells					678:684	MIN6 β-cells	673:684	MIN6 β-cells	673:684	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	1	27	theme	diabetes	211:218	arg1	mellitus					220:227	type 1 diabetes mellitus	204:227	type 1 diabetes mellitus	204:227	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	3	28	theme	islet	390:394	arg1	material					430:437	a novel islet cells biomimetic microencapsulant material	382:437	a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC)	382:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	6	29	theme	culture	979:985	arg1	week					971:974	1 week	969:974	1 week of culture	969:985	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	2	30	theme	functionality	332:344	arg1	loss					304:307	the peri-transplantation loss	279:307	the peri-transplantation loss of islet viability and functionality in the microcapsules	279:365	However, its clinical translation is hampered by the peri-transplantation loss of islet viability and functionality in the microcapsules.					
32815257	1	31	theme	mellitus	220:227	arg1	treatment					191:199	the treatment	187:199	the treatment of type 1 diabetes mellitus	187:227	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	3	32	theme	cells	396:400	arg1	material					430:437	a novel islet cells biomimetic microencapsulant material	382:437	a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC)	382:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	5	33	theme	ionotropic	707:716	arg1	gelation					718:725	ionotropic gelation	707:725	ionotropic gelation of the alginate matrix	707:748	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	6	34	theme	AEC	894:896	arg1	≈640 µm					913:919	≈640 µm	913:919	≈640 µm	913:919	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	6	34	theme	AEC	894:896	arg1	microcapsules					898:910	the AEC microcapsules	890:910	the AEC microcapsules (≈640 µm)	890:920	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	1	35	theme	microencapsulated	131:147	arg1	cells					155:159	microencapsulated islet cells	131:159	microencapsulated islet cells	131:159	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	3	36	theme	extracellular	502:514	arg1	ECM					524:526	ECM	524:526	ECM	524:526	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	3	36	theme	extracellular	502:514	arg1	matrix					516:521	extracellular matrix	502:521	extracellular matrix (ECM)	502:527	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	0	37	theme	Extracellular	51:63	arg1	Hydrogel					72:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	7	38	theme	AEC	1078:1080	arg1	microcapsule					1082:1093	the AEC microcapsule	1074:1093	the AEC microcapsule	1074:1093	The results show that the AEC microcapsule is a potent platform to bioaugment the performance of islet cells.					
32815257	7	38	theme	AEC	1078:1080	arg1	platform					1107:1114	a potent platform	1098:1114	a potent platform to bioaugment the performance of islet cells	1098:1159	The results show that the AEC microcapsule is a potent platform to bioaugment the performance of islet cells.					
32815257	7	39	theme	cells	1155:1159	arg1	performance					1134:1144	the performance	1130:1144	the performance of islet cells	1130:1159	The results show that the AEC microcapsule is a potent platform to bioaugment the performance of islet cells.					
32815257	0	40	theme	Adipose	43:49	arg1	Hydrogel					72:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	5	41	theme	ECM	805:807	arg1	pre-gel					809:815	the pepsin-solubilized ECM pre-gel	782:815	the pepsin-solubilized ECM pre-gel	782:815	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	0	42	theme	Alginate-Human	28:41	arg1	Hydrogel					72:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	3	43	theme	alginate	489:496	arg1	ECM					524:526	ECM	524:526	ECM	524:526	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	3	43	theme	alginate	489:496	arg1	matrix					516:521	extracellular matrix	502:521	extracellular matrix (ECM)	502:527	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	3	43	theme	alginate	489:496	arg1	networks					477:484	the interpenetrating networks	456:484	the interpenetrating networks of alginate	456:496	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	0	44	theme	Hydrogel	72:79	arg1	Network					17:23	Interpenetrating Network	0:23	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells	0:95	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	7	45	theme	potent	1100:1105	arg1	microcapsule					1082:1093	the AEC microcapsule	1074:1093	the AEC microcapsule	1074:1093	The results show that the AEC microcapsule is a potent platform to bioaugment the performance of islet cells.					
32815257	7	45	theme	potent	1100:1105	arg1	platform					1107:1114	a potent platform	1098:1114	a potent platform to bioaugment the performance of islet cells	1098:1159	The results show that the AEC microcapsule is a potent platform to bioaugment the performance of islet cells.					
32815257	2	46	theme	clinical	243:250	arg1	translation					252:262	its clinical translation	239:262	its clinical translation	239:262	However, its clinical translation is hampered by the peri-transplantation loss of islet viability and functionality in the microcapsules.					
32815257	1	47	theme	islet	149:153	arg1	cells					155:159	microencapsulated islet cells	131:159	microencapsulated islet cells	131:159	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	0	48	theme	Matrix	65:70	arg1	Hydrogel					72:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Alginate-Human Adipose Extracellular Matrix Hydrogel	28:79	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	5	49	theme	pancreatic	647:656	arg1	cells					666:670	pancreatic β islet cells	647:670	pancreatic β islet cells (MIN6 β-cells)	647:685	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	49	theme	pancreatic	647:656	arg1	β-cells					678:684	MIN6 β-cells	673:684	MIN6 β-cells	673:684	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	3	50	dep	networks	477:484	arg1	composite					538:546	hydrogel composite	529:546	hydrogel composite (AEC)	529:552	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	3	50	dep	networks	477:484	arg1	AEC					549:551	AEC	549:551	AEC	549:551	In this work, a novel islet cells biomimetic microencapsulant material that is based on the interpenetrating networks of alginate and extracellular matrix (ECM) hydrogel composite (AEC) is presented.					
32815257	1	51	theme	cells	155:159	arg1	Transplantation					112:126	Transplantation	112:126	Transplantation of microencapsulated islet cells	112:159	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	7	52	theme	islet	1149:1153	arg1	cells					1155:1159	islet cells	1149:1159	islet cells	1149:1159	The results show that the AEC microcapsule is a potent platform to bioaugment the performance of islet cells.					
32815257	6	53	theme	cell	952:955	arg1	growth					957:962	cell growth	952:962	cell growth	952:962	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	4	54	attach	derived	589:595	arg1	lipoaspirate					608:619	human lipoaspirate	602:619	human lipoaspirate	602:619	The ECM component is derived from human lipoaspirate.					
32815257	4	54	attach	derived	589:595	arg2	component					576:584	The ECM component	568:584	The ECM component	568:584	The ECM component is derived from human lipoaspirate.					
32815257	2	55	theme	peri-transplantation	283:302	arg1	loss					304:307	the peri-transplantation loss	279:307	the peri-transplantation loss of islet viability and functionality in the microcapsules	279:365	However, its clinical translation is hampered by the peri-transplantation loss of islet viability and functionality in the microcapsules.					
32815257	2	56	from	loss	304:307	arg1	microcapsules					353:365	the microcapsules	349:365	the microcapsules	349:365	However, its clinical translation is hampered by the peri-transplantation loss of islet viability and functionality in the microcapsules.					
32815257	1	57	theme	great	167:171	arg1	potential					173:181	great potential	167:181	great potential	167:181	Transplantation of microencapsulated islet cells holds great potential for the treatment of type 1 diabetes mellitus.					
32815257	0	58	theme	Islet	85:89	arg1	Cells					91:95	Islet Cells	85:95	Islet Cells	85:95	Interpenetrating Network of Alginate-Human Adipose Extracellular Matrix Hydrogel for Islet Cells Encapsulation.					
32815257	6	59	theme	cell-matrix	838:848	arg1	interaction					850:860	the enhanced cell-matrix interaction	825:860	the enhanced cell-matrix interaction	825:860	Due to the enhanced cell-matrix interaction, islets encapsulated within the AEC microcapsules (≈640 µm) display sevenfold increase in cell growth over 1 week of culture and characteristic glucose-stimulated insulin response in vitro.					
32815257	5	60	dep	In	622:623	arg1	situ					625:628	situ	625:628	situ	625:628	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	61	theme	pre-gel	809:815	arg1	gelation					770:777	thermal-induced gelation	754:777	thermal-induced gelation of the pepsin-solubilized ECM pre-gel	754:815	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	61	theme	pre-gel	809:815	arg1	gelation					718:725	ionotropic gelation	707:725	ionotropic gelation of the alginate matrix	707:748	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
32815257	5	62	theme	alginate	734:741	arg1	matrix					743:748	the alginate matrix	730:748	the alginate matrix	730:748	In situ encapsulation of pancreatic β islet cells (MIN6 β-cells) can be achieved via ionotropic gelation of the alginate matrix and thermal-induced gelation of the pepsin-solubilized ECM pre-gel.					
34579126	8	0	theme	Specific	1159:1166	arg1	colonization					1168:1179	Specific colonization	1159:1179	Specific colonization of B. breve	1159:1191	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	4	1	theme	cells	863:867	arg1	co-culture					817:826	a co-culture	815:826	a co-culture of enterocytes and macrophage-like cells	815:867	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	9	2	theme	barrier	1416:1422	arg1	improvement					1367:1377	a significant improvement	1353:1377	a significant improvement of inflammation-disrupted epithelial barrier	1353:1422	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	2	theme	barrier	1416:1422	arg1	shifts					1428:1433	shifts	1428:1433	shifts on cytokine production	1428:1456	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	3	3	theme	chitin-glucan	474:486	arg1	effect					464:469	the effect	460:469	the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota	460:566	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	4	4	from	effect	643:648	arg1	fermentation					706:717	CG and CGB fermentation	695:717	CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	695:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	0	5	theme	Epithelial	72:81	arg1	Barrier					83:89	Epithelial Barrier	72:89	Epithelial Barrier	72:89	Chitin Glucan Shifts Luminal and Mucosal Microbial Communities, Improve Epithelial Barrier and Modulates Cytokine Production In Vitro.					
34579126	4	6	theme	macrophage-like	847:861	arg1	cells					863:867	macrophage-like cells	847:867	macrophage-like cells	847:867	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	7	7	theme	donors	1094:1099	arg1	two					1081:1083	two	1081:1083	two	1081:1083	In two of three donors, CG and CGB also stimulated Faecalibacterium prausniitzi.					
34579126	7	7	theme	donors	1094:1099	arg1	donors					1094:1099	three donors	1088:1099	three donors	1088:1099	In two of three donors, CG and CGB also stimulated Faecalibacterium prausniitzi.					
34579126	9	8	from	improvement	1367:1377	arg1	production					1447:1456	cytokine production	1438:1456	cytokine production	1438:1456	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	9	theme	cytokine	1438:1445	arg1	production					1447:1456	cytokine production	1438:1456	cytokine production	1438:1456	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	4	10	theme	inflammation-induced	745:764	arg1	disruption					774:783	inflammation-induced barrier disruption	745:783	inflammation-induced barrier disruption	745:783	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	4	11	from	effects	734:740	arg1	disruption					774:783	inflammation-induced barrier disruption	745:783	inflammation-induced barrier disruption	745:783	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	4	11	from	effects	734:740	arg1	production					798:807	cytokine production	789:807	cytokine production	789:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	4	12	theme	protective	723:732	arg1	effects					734:740	protective effects	723:740	protective effects on inflammation-induced barrier disruption and cytokine production	723:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	5	13	theme	shift	954:958	arg1	composition					981:991	shift human gut microbiota composition	954:991	shift human gut microbiota composition	954:991	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	5	14	theme	human	960:964	arg1	composition					981:991	shift human gut microbiota composition	954:991	shift human gut microbiota composition	954:991	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	3	15	theme	Bifidobacterium	516:530	arg1	breve					532:536	Bifidobacterium breve	516:536	Bifidobacterium breve	516:536	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	2	16	theme	prebiotic	262:270	arg1	interventions					272:284	pro- and prebiotic interventions	253:284	pro- and prebiotic interventions	253:284	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	9	17	dep	cytokines	1517:1525	arg1	IL6					1536:1538	IL6	1536:1538	IL6	1536:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	17	dep	cytokines	1517:1525	arg1	IL10					1527:1530	IL10	1527:1530	IL10	1527:1530	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	17	dep	cytokines	1517:1525	arg1	cytokines					1517:1525	the immunomodulatory cytokines	1496:1525	the immunomodulatory cytokines IL10 and IL6	1496:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	4	18	theme	supernatants	677:688	arg1	effect					643:648	the effect	639:648	the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	639:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	8	19	theme	breve	1187:1191	arg1	colonization					1168:1179	Specific colonization	1159:1179	Specific colonization of B. breve	1159:1191	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	6	20	theme	butyrate	1049:1056	arg1	producing-bacteria					1058:1075	butyrate producing-bacteria	1049:1075	butyrate producing-bacteria	1049:1075	and butyrate producing-bacteria.					
34579126	5	21	theme	microbiota	970:979	arg1	composition					981:991	shift human gut microbiota composition	954:991	shift human gut microbiota composition	954:991	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	0	22	theme	Cytokine	105:112	arg1	Production					114:123	Cytokine Production	105:123	Cytokine Production	105:123	Chitin Glucan Shifts Luminal and Mucosal Microbial Communities, Improve Epithelial Barrier and Modulates Cytokine Production In Vitro.					
34579126	4	23	from	fermentation	706:717	arg1	effect					643:648	the effect	639:648	the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	639:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	4	23	from	fermentation	706:717	arg1	supernatants					677:688	filter-sterilized fecal supernatants	653:688	filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	653:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	2	24	theme	pro-	253:256	arg1	interventions					272:284	pro- and prebiotic interventions	253:284	pro- and prebiotic interventions	253:284	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	9	25	from	increase	1484:1491	arg1	IL6					1536:1538	IL6	1536:1538	IL6	1536:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	25	from	increase	1484:1491	arg1	IL10					1527:1530	IL10	1527:1530	IL10	1527:1530	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	25	from	increase	1484:1491	arg1	cytokines					1517:1525	the immunomodulatory cytokines	1496:1525	the immunomodulatory cytokines IL10 and IL6	1496:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	2	26	from	effect	330:335	arg1	interplay					404:412	gut microbiota-host interplay	384:412	gut microbiota-host interplay	384:412	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	5	27	theme	acid	934:937	arg1	production					939:948	health-beneficial short-chain fatty acid production	898:948	health-beneficial short-chain fatty acid production	898:948	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	9	28	theme	consistent	1473:1482	arg1	increase					1484:1491	consistent increase	1473:1491	consistent increase in the immunomodulatory cytokines IL10 and IL6	1473:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	0	29	theme	Chitin	0:5	arg1	Glucan					7:12	Chitin Glucan	0:12	Chitin Glucan	0:12	Chitin Glucan Shifts Luminal and Mucosal Microbial Communities, Improve Epithelial Barrier and Modulates Cytokine Production In Vitro.					
34579126	4	30	theme	enterocytes	831:841	arg1	co-culture					817:826	a co-culture	815:826	a co-culture of enterocytes and macrophage-like cells	815:867	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	3	31	from	effect	464:469	arg1	microbiota					557:566	human gut microbiota	547:566	human gut microbiota	547:566	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	2	32	theme	promising	298:306	arg1	results					308:314	promising results	298:314	promising results	298:314	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	0	33	theme	Luminal	21:27	arg1	Communities					51:61	Luminal and Mucosal Microbial Communities	21:61	Luminal and Mucosal Microbial Communities	21:61	Chitin Glucan Shifts Luminal and Mucosal Microbial Communities, Improve Epithelial Barrier and Modulates Cytokine Production In Vitro.					
34579126	5	34	theme	fatty	928:932	arg1	acid					934:937	health-beneficial short-chain fatty acid	898:937	health-beneficial short-chain fatty acid production	898:948	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	9	35	theme	inflammation-disrupted	1382:1403	arg1	barrier					1416:1422	inflammation-disrupted epithelial barrier	1382:1422	inflammation-disrupted epithelial barrier	1382:1422	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	2	36	theme	microbiota-host	388:402	arg1	interplay					404:412	gut microbiota-host interplay	384:412	gut microbiota-host interplay	384:412	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	4	37	theme	CG	695:696	arg1	fermentation					706:717	CG and CGB fermentation	695:717	CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	695:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	1	38	attach	linked	169:174	arg1	status					190:195	the health status	179:195	the health status of the host	179:207	The human gut microbiota has been linked to the health status of the host.					
34579126	1	38	attach	linked	169:174	arg2	microbiota					149:158	The human gut microbiota	135:158	The human gut microbiota	135:158	The human gut microbiota has been linked to the health status of the host.					
34579126	4	39	theme	CGB	702:704	arg1	fermentation					706:717	CG and CGB fermentation	695:717	CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	695:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	2	40	theme	gut	384:386	arg1	interplay					404:412	gut microbiota-host interplay	384:412	gut microbiota-host interplay	384:412	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	0	41	theme	Mucosal	33:39	arg1	Communities					51:61	Luminal and Mucosal Microbial Communities	21:61	Luminal and Mucosal Microbial Communities	21:61	Chitin Glucan Shifts Luminal and Mucosal Microbial Communities, Improve Epithelial Barrier and Modulates Cytokine Production In Vitro.					
34579126	5	42	theme	health-beneficial	898:914	arg1	acid					934:937	health-beneficial short-chain fatty acid	898:937	health-beneficial short-chain fatty acid production	898:948	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	3	43	theme	gut	553:555	arg1	microbiota					557:566	human gut microbiota	547:566	human gut microbiota	547:566	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	9	44	theme	immunomodulatory	1500:1515	arg1	IL6					1536:1538	IL6	1536:1538	IL6	1536:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	44	theme	immunomodulatory	1500:1515	arg1	IL10					1527:1530	IL10	1527:1530	IL10	1527:1530	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	44	theme	immunomodulatory	1500:1515	arg1	cytokines					1517:1525	the immunomodulatory cytokines	1496:1525	the immunomodulatory cytokines IL10 and IL6	1496:1538	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	9	45	theme	significant	1355:1365	arg1	improvement					1367:1377	a significant improvement	1353:1377	a significant improvement of inflammation-disrupted epithelial barrier	1353:1422	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	8	46	dep	lumen	1213:1217	arg1	the					1209:1211	the	1209:1211	the	1209:1211	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	3	47	theme	mucosal	589:595	arg1	environments					597:608	the luminal and mucosal environments	573:608	the luminal and mucosal environments	573:608	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	4	48	theme	cytokine	789:796	arg1	production					798:807	cytokine production	789:807	cytokine production	789:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	8	49	theme	B.	1184:1185	arg1	breve					1187:1191	B. breve	1184:1191	B. breve	1184:1191	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	7	50	theme	Faecalibacterium	1129:1144	arg1	prausniitzi					1146:1156	Faecalibacterium prausniitzi	1129:1156	Faecalibacterium prausniitzi	1129:1156	In two of three donors, CG and CGB also stimulated Faecalibacterium prausniitzi.					
34579126	5	51	theme	Roseburia	1030:1038	arg1	spp					1040:1042	Roseburia spp	1030:1042	Roseburia spp	1030:1042	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	4	52	theme	fecal	671:675	arg1	supernatants					677:688	filter-sterilized fecal supernatants	653:688	filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	653:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	0	53	theme	Microbial	41:49	arg1	Communities					51:61	Luminal and Mucosal Microbial Communities	21:61	Luminal and Mucosal Microbial Communities	21:61	Chitin Glucan Shifts Luminal and Mucosal Microbial Communities, Improve Epithelial Barrier and Modulates Cytokine Production In Vitro.					
34579126	9	54	from	shifts	1428:1433	arg1	production					1447:1456	cytokine production	1438:1456	cytokine production	1438:1456	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	3	55	theme	human	547:551	arg1	microbiota					557:566	human gut microbiota	547:566	human gut microbiota	547:566	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	4	56	theme	filter-sterilized	653:669	arg1	supernatants					677:688	filter-sterilized fecal supernatants	653:688	filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production	653:807	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	5	57	theme	consistent	1001:1010	arg1	effect					1012:1017	a consistent effect	999:1017	a consistent effect	999:1017	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	1	58	theme	human	139:143	arg1	microbiota					149:158	The human gut microbiota	135:158	The human gut microbiota	135:158	The human gut microbiota has been linked to the health status of the host.					
34579126	3	59	theme	luminal	577:583	arg1	environments					597:608	the luminal and mucosal environments	573:608	the luminal and mucosal environments	573:608	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	8	60	located	observed	1197:1204	arg2	colonization					1168:1179	Specific colonization	1159:1179	Specific colonization of B. breve	1159:1191	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	8	60	located	observed	1197:1204	arg1	lumen					1213:1217	lumen	1213:1217	lumen	1213:1217	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	8	60	located	observed	1197:1204	arg1	compartment					1231:1241	mucosal compartment	1223:1241	mucosal compartment	1223:1241	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	5	61	theme	short-chain	916:926	arg1	acid					934:937	health-beneficial short-chain fatty acid	898:937	health-beneficial short-chain fatty acid production	898:948	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	9	62	theme	epithelial	1405:1414	arg1	barrier					1416:1422	inflammation-disrupted epithelial barrier	1382:1422	inflammation-disrupted epithelial barrier	1382:1422	Both treatments included a significant improvement of inflammation-disrupted epithelial barrier and shifts on cytokine production, especially by consistent increase in the immunomodulatory cytokines IL10 and IL6.					
34579126	1	63	theme	host	204:207	arg1	status					190:195	the health status	179:195	the health status of the host	179:207	The human gut microbiota has been linked to the health status of the host.					
34579126	3	64	theme	chitin-glucan	497:509	arg1	effect					464:469	the effect	460:469	the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota	460:566	We assessed the effect of chitin-glucan (CG) and chitin-glucan plus Bifidobacterium breve (CGB) on human gut microbiota from the luminal and mucosal environments in vitro.					
34579126	8	65	theme	mucosal	1223:1229	arg1	compartment					1231:1241	mucosal compartment	1223:1241	mucosal compartment	1223:1241	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
34579126	2	66	theme	microbiota	234:243	arg1	Modulation					210:219	Modulation	210:219	Modulation of human gut microbiota through pro- and prebiotic interventions	210:284	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	1	67	theme	gut	145:147	arg1	microbiota					149:158	The human gut microbiota	135:158	The human gut microbiota	135:158	The human gut microbiota has been linked to the health status of the host.					
34579126	4	68	theme	barrier	766:772	arg1	disruption					774:783	inflammation-induced barrier disruption	745:783	inflammation-induced barrier disruption	745:783	Further, we tested the effect of filter-sterilized fecal supernatants from CG and CGB fermentation for protective effects on inflammation-induced barrier disruption and cytokine production using a co-culture of enterocytes and macrophage-like cells.					
34579126	2	69	theme	gut	230:232	arg1	microbiota					234:243	human gut microbiota	224:243	human gut microbiota	224:243	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	1	70	theme	health	183:188	arg1	status					190:195	the health status	179:195	the health status of the host	179:207	The human gut microbiota has been linked to the health status of the host.					
34579126	2	71	theme	prebiotics	346:355	arg1	effect					330:335	the effect	326:335	the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay	326:412	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	5	72	theme	gut	966:968	arg1	composition					981:991	shift human gut microbiota composition	954:991	shift human gut microbiota composition	954:991	Overall, CG and CGB promote health-beneficial short-chain fatty acid production and shift human gut microbiota composition, with a consistent effect increasing Roseburia spp.					
34579126	2	73	theme	human	224:228	arg1	microbiota					234:243	human gut microbiota	224:243	human gut microbiota	224:243	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	2	74	theme	novel	340:344	arg1	chitin-glucan					366:378	chitin-glucan	366:378	chitin-glucan	366:378	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	2	74	theme	novel	340:344	arg1	prebiotics					346:355	novel prebiotics	340:355	novel prebiotics	340:355	Modulation of human gut microbiota through pro- and prebiotic interventions has yielded promising results; however, the effect of novel prebiotics, such as chitin-glucan, on gut microbiota-host interplay is still not fully characterized.					
34579126	8	75	theme	different	1281:1289	arg1	endpoints					1291:1299	different endpoints	1281:1299	different endpoints	1281:1299	Specific colonization of B. breve was observed in the lumen and mucosal compartment; however, no synergy was detected for different endpoints when comparing CGB and CG.					
35148865	0	0	theme	tissue	113:118	arg1	regeneration					120:131	bone tissue regeneration	108:131	bone tissue regeneration	108:131	Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration.					
35148865	6	1	theme	skull	983:987	arg1	model					1010:1014	a rat skull critical-size defect model	977:1014	a rat skull critical-size defect model	977:1014	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	6	2	theme	scaffolds	947:955	arg1	evaluation					895:904	An In vivo evaluation	884:904	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds	884:955	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	9	3	dep	In	1309:1310	arg1	vivo					1312:1315	vivo	1312:1315	vivo	1312:1315	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	2	4	with	functionalization	346:362	arg1	cells					388:392	cells	388:392	cells	388:392	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	0	5	theme	bone	108:111	arg1	regeneration					120:131	bone tissue regeneration	108:131	bone tissue regeneration	108:131	Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration.					
35148865	6	6	theme	rat	979:981	arg1	model					1010:1014	a rat skull critical-size defect model	977:1014	a rat skull critical-size defect model	977:1014	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	7	7	theme	osteogenic	1158:1167	arg1	differentiation					1169:1183	osteogenic differentiation	1158:1183	osteogenic differentiation ability	1158:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	10	8	theme	transplantable	1527:1540	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	8	theme	transplantable	1527:1540	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	9	theme	tissue	1574:1579	arg1	engineering					1581:1591	bone tissue engineering	1569:1591	bone tissue engineering	1569:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	10	theme	promising	1517:1525	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	10	theme	promising	1517:1525	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	3	11	theme	hyaluronic	591:600	arg1	HyA-Gel/BCP					643:653	HyA-Gel/BCP	643:653	HyA-Gel/BCP	643:653	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	3	11	theme	hyaluronic	591:600	arg1	phosphate					632:640	hyaluronic acid/gelatin-biphasic calcium phosphate	591:640	hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration	591:686	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	7	12	with	like-cells	1142:1151	arg1	ability					1185:1191	osteogenic differentiation ability	1158:1191	osteogenic differentiation ability	1158:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	10	13	theme	bone	1569:1572	arg1	engineering					1581:1591	bone tissue engineering	1569:1591	bone tissue engineering	1569:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	3	14	theme	heterogeneous	500:512	arg1	cells					514:518	stromal vascular fraction (SVF) heterogeneous cells	468:518	stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue	468:572	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	9	15	theme	unloaded	1424:1431	arg1	scaffolds					1445:1453	unloaded HyA-Gel/BCP scaffolds	1424:1453	unloaded HyA-Gel/BCP scaffolds	1424:1453	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	2	16	theme	bone	328:331	arg1	defects					333:339	filling bone defects	320:339	filling bone defects	320:339	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	2	17	theme	ideal	434:438	arg1	sources					445:451	ideal cell sources	434:451	ideal cell sources	434:451	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	5	18	theme	in	857:858	arg1	characterization					866:881	in vitro characterization	857:881	in vitro characterization	857:881	Then, they were cultured on HyA-Gel/BCP scaffolds for in vitro characterization.					
35148865	9	19	theme	HyA-Gel/BCP	1433:1443	arg1	scaffolds					1445:1453	unloaded HyA-Gel/BCP scaffolds	1424:1453	unloaded HyA-Gel/BCP scaffolds	1424:1453	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	5	20	theme	HyA-Gel/BCP	831:841	arg1	scaffolds					843:851	HyA-Gel/BCP scaffolds	831:851	HyA-Gel/BCP scaffolds	831:851	Then, they were cultured on HyA-Gel/BCP scaffolds for in vitro characterization.					
35148865	2	21	theme	filling	320:326	arg1	defects					333:339	filling bone defects	320:339	filling bone defects	320:339	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	10	22	theme	SVF-loaded	1473:1482	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	22	theme	SVF-loaded	1473:1482	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	1	23	theme	unavoidable	206:216	arg1	limitations					218:228	the unavoidable limitations	202:228	the unavoidable limitations of auto- and allografting	202:254	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	3	24	theme	adipose	559:565	arg1	tissue					567:572	autologous adipose tissue	548:572	autologous adipose tissue	548:572	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	1	25	with	augmentation	146:157	arg1	materials					174:182	synthetic materials	164:182	synthetic materials	164:182	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	3	26	theme	acid/gelatin-biphasic	602:622	arg1	HyA-Gel/BCP					643:653	HyA-Gel/BCP	643:653	HyA-Gel/BCP	643:653	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	3	26	theme	acid/gelatin-biphasic	602:622	arg1	phosphate					632:640	hyaluronic acid/gelatin-biphasic calcium phosphate	591:640	hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration	591:686	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	8	27	theme	HyA-Gel/BCP	1252:1262	arg1	scaffolds					1264:1272	the HyA-Gel/BCP scaffolds	1248:1272	the HyA-Gel/BCP scaffolds	1248:1272	Also, the SVF cells could be cultured and expanded on the HyA-Gel/BCP scaffolds without affecting their viability.					
35148865	6	28	theme	In	887:888	arg1	evaluation					895:904	An In vivo evaluation	884:904	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds	884:955	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	10	29	theme	grafting	1547:1554	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	29	theme	grafting	1547:1554	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	3	30	theme	calcium	624:630	arg1	HyA-Gel/BCP					643:653	HyA-Gel/BCP	643:653	HyA-Gel/BCP	643:653	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	3	30	theme	calcium	624:630	arg1	phosphate					632:640	hyaluronic acid/gelatin-biphasic calcium phosphate	591:640	hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration	591:686	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	6	31	theme	SVF-loaded	924:933	arg1	scaffolds					947:955	the autologous SVF-loaded HyA-Gel/BCP scaffolds	909:955	the autologous SVF-loaded HyA-Gel/BCP scaffolds	909:955	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	6	32	dep	In	887:888	arg1	vivo					890:893	vivo	890:893	vivo	890:893	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	9	33	theme	In	1309:1310	arg1	implantation					1317:1328	In vivo implantation	1309:1328	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds	1309:1375	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	1	34	theme	Bone	134:137	arg1	augmentation					146:157	Bone defect augmentation	134:157	Bone defect augmentation with synthetic materials	134:182	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	1	35	theme	auto-	233:237	arg1	limitations					218:228	the unavoidable limitations	202:228	the unavoidable limitations of auto- and allografting	202:254	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	3	36	theme	autologous	548:557	arg1	tissue					567:572	autologous adipose tissue	548:572	autologous adipose tissue	548:572	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	6	37	theme	HyA-Gel/BCP	935:945	arg1	scaffolds					947:955	the autologous SVF-loaded HyA-Gel/BCP scaffolds	909:955	the autologous SVF-loaded HyA-Gel/BCP scaffolds	909:955	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	1	38	theme	defect	139:144	arg1	augmentation					146:157	Bone defect augmentation	134:157	Bone defect augmentation with synthetic materials	134:182	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	3	39	theme	vascular	476:483	arg1	cells					514:518	stromal vascular fraction (SVF) heterogeneous cells	468:518	stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue	468:572	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	0	40	theme	acid/gelatin-biphasic	55:75	arg1	scaffold					95:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold	44:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration	44:131	Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration.					
35148865	7	41	theme	mesenchymal	1125:1135	arg1	like-cells					1142:1151	mesenchymal stem like-cells	1125:1151	mesenchymal stem like-cells with osteogenic differentiation ability	1125:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	1	42	theme	allografting	243:254	arg1	limitations					218:228	the unavoidable limitations	202:228	the unavoidable limitations of auto- and allografting	202:254	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	2	43	theme	different	276:284	arg1	materials					306:314	different promising synthetic materials	276:314	different promising synthetic materials for filling bone defects	276:339	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	2	44	theme	sources	445:451	arg1	lack					426:429	the lack	422:429	the lack of ideal cell sources	422:451	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	0	45	theme	hyaluronic	44:53	arg1	scaffold					95:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold	44:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration	44:131	Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration.					
35148865	7	46	theme	stem	1137:1140	arg1	like-cells					1142:1151	mesenchymal stem like-cells	1125:1151	mesenchymal stem like-cells with osteogenic differentiation ability	1125:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	6	47	theme	autologous	913:922	arg1	scaffolds					947:955	the autologous SVF-loaded HyA-Gel/BCP scaffolds	909:955	the autologous SVF-loaded HyA-Gel/BCP scaffolds	909:955	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	7	48	theme	cells	1108:1112	arg1	like-cells					1142:1151	mesenchymal stem like-cells	1125:1151	mesenchymal stem like-cells with osteogenic differentiation ability	1125:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	7	48	theme	cells	1108:1112	arg1	types					1099:1103	different types	1089:1103	different types	1089:1103	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	7	49	theme	different	1089:1097	arg1	like-cells					1142:1151	mesenchymal stem like-cells	1125:1151	mesenchymal stem like-cells with osteogenic differentiation ability	1125:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	7	49	theme	different	1089:1097	arg1	types					1099:1103	different types	1089:1103	different types	1089:1103	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	2	50	theme	cell	440:443	arg1	sources					445:451	ideal cell sources	434:451	ideal cell sources	434:451	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	0	51	theme	phosphate	85:93	arg1	scaffold					95:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold	44:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration	44:131	Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration.					
35148865	9	52	theme	autologous	1333:1342	arg1	scaffolds					1367:1375	autologous SVF-loaded HyA-Gel/BCP scaffolds	1333:1375	autologous SVF-loaded HyA-Gel/BCP scaffolds	1333:1375	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	3	53	theme	phosphate	632:640	arg1	scaffolds					656:664	hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds	591:664	hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration	591:686	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	4	54	theme	differentiation	762:776	arg1	potential					778:786	osteogenic differentiation potential	751:786	osteogenic differentiation potential	751:786	The SVF cells were isolated, and the cellular composition and osteogenic differentiation potential were analyzed.					
35148865	0	55	theme	calcium	77:83	arg1	scaffold					95:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold	44:102	hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration	44:131	Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration.					
35148865	9	56	theme	excellent	1384:1392	arg1	regeneration					1399:1410	excellent bone regeneration	1384:1410	excellent bone regeneration	1384:1410	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	9	57	theme	SVF-loaded	1344:1353	arg1	scaffolds					1367:1375	autologous SVF-loaded HyA-Gel/BCP scaffolds	1333:1375	autologous SVF-loaded HyA-Gel/BCP scaffolds	1333:1375	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	5	58	dep	in	857:858	arg1	vitro					860:864	vitro	860:864	vitro	860:864	Then, they were cultured on HyA-Gel/BCP scaffolds for in vitro characterization.					
35148865	9	59	theme	bone	1394:1397	arg1	regeneration					1399:1410	excellent bone regeneration	1384:1410	excellent bone regeneration	1384:1410	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	4	60	theme	osteogenic	751:760	arg1	potential					778:786	osteogenic differentiation potential	751:786	osteogenic differentiation potential	751:786	The SVF cells were isolated, and the cellular composition and osteogenic differentiation potential were analyzed.					
35148865	6	61	theme	defect	1003:1008	arg1	model					1010:1014	a rat skull critical-size defect model	977:1014	a rat skull critical-size defect model	977:1014	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	8	62	theme	SVF	1204:1206	arg1	cells					1208:1212	the SVF cells	1200:1212	the SVF cells	1200:1212	Also, the SVF cells could be cultured and expanded on the HyA-Gel/BCP scaffolds without affecting their viability.					
35148865	9	63	theme	HyA-Gel/BCP	1355:1365	arg1	scaffolds					1367:1375	autologous SVF-loaded HyA-Gel/BCP scaffolds	1333:1375	autologous SVF-loaded HyA-Gel/BCP scaffolds	1333:1375	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	3	64	theme	bone	670:673	arg1	regeneration					675:686	bone regeneration	670:686	bone regeneration	670:686	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	1	65	theme	synthetic	164:172	arg1	materials					174:182	synthetic materials	164:182	synthetic materials	164:182	Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting.					
35148865	3	66	used	used	463:466	arg2	we					460:461	we	460:461	we	460:461	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	9	67	theme	scaffolds	1367:1375	arg1	implantation					1317:1328	In vivo implantation	1309:1328	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds	1309:1375	In vivo implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds.					
35148865	10	68	theme	autologous	1462:1471	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	68	theme	autologous	1462:1471	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	2	69	theme	materials	373:381	arg1	functionalization					346:362	the functionalization	342:362	the functionalization of these materials with cells	342:392	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	7	70	theme	differentiation	1169:1183	arg1	ability					1185:1191	osteogenic differentiation ability	1158:1191	osteogenic differentiation ability	1158:1191	The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability.					
35148865	4	71	theme	SVF	693:695	arg1	cells					697:701	The SVF cells	689:701	The SVF cells	689:701	The SVF cells were isolated, and the cellular composition and osteogenic differentiation potential were analyzed.					
35148865	2	72	theme	synthetic	296:304	arg1	materials					306:314	different promising synthetic materials	276:314	different promising synthetic materials for filling bone defects	276:339	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	4	73	theme	cellular	726:733	arg1	composition					735:745	the cellular composition	722:745	the cellular composition	722:745	The SVF cells were isolated, and the cellular composition and osteogenic differentiation potential were analyzed.					
35148865	3	74	theme	stromal	468:474	arg1	cells					514:518	stromal vascular fraction (SVF) heterogeneous cells	468:518	stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue	468:572	Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration.					
35148865	10	75	theme	bone	1542:1545	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	75	theme	bone	1542:1545	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	6	76	theme	critical-size	989:1001	arg1	model					1010:1014	a rat skull critical-size defect model	977:1014	a rat skull critical-size defect model	977:1014	An In vivo evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model.					
35148865	2	77	theme	promising	286:294	arg1	materials					306:314	different promising synthetic materials	276:314	different promising synthetic materials for filling bone defects	276:339	Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources.					
35148865	10	78	theme	HyA-Gel/BCP	1484:1494	arg1	material					1556:1563	a promising transplantable bone grafting material	1515:1563	a promising transplantable bone grafting material for bone tissue engineering	1515:1591	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35148865	10	78	theme	HyA-Gel/BCP	1484:1494	arg1	scaffolds					1496:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	autologous SVF-loaded HyA-Gel/BCP scaffolds	1462:1504	Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.					
35527137	7	0	theme	gradient	1144:1151	arg1	constructs					1162:1171	the acellular 3D gradient hydrogel constructs	1127:1171	the acellular 3D gradient hydrogel constructs	1127:1171	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	13	1	theme	engineered	2070:2079	arg1	milieu					2093:2098	the dissimilar engineered biophysical milieu	2055:2098	the dissimilar engineered biophysical milieu	2055:2098	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	8	2	theme	3D	1189:1190	arg1	constructs					1201:1210	The cell-based 3D hydrogel constructs	1174:1210	The cell-based 3D hydrogel constructs	1174:1210	The cell-based 3D hydrogel constructs were assessed for MSCs viability by live/dead staining.					
35527137	2	3	from	function	369:376	arg1	interface					298:306	The tissue-to-tissue interface	277:306	The tissue-to-tissue interface connecting two adjacent tissues	277:338	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	2	3	from	function	369:376	arg1	vital					343:347	vital	343:347	vital	343:347	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	4	4	theme	extracellular	667:679	arg1	ECM					689:691	ECM	689:691	ECM	689:691	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	4	4	theme	extracellular	667:679	arg1	matrix					681:686	the extracellular matrix	663:686	the extracellular matrix (ECM)	663:692	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	7	5	theme	acellular	1131:1139	arg1	constructs					1162:1171	the acellular 3D gradient hydrogel constructs	1127:1171	the acellular 3D gradient hydrogel constructs	1127:1171	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	12	6	theme	human	1841:1845	arg1	MSCs					1847:1850	the human MSCs	1837:1850	tuning the human MSCs morphology	1830:1861	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	0	7	theme	Macromolecular	0:13	arg1	modulation					15:24	Macromolecular modulation	0:24	Macromolecular modulation of a 3D hydrogel construct	0:51	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	4	8	theme	Col	728:730	arg1	collagen					718:725	type I collagen	711:725	type I collagen (Col I)	711:733	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	4	8	theme	Col	728:730	arg1	I					732:732	Col I	728:732	Col I	728:732	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	7	9	theme	mechanical	1091:1100	arg1	properties					1102:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	2	10	theme	effective	352:360	arg1	function					369:376	effective tissue function	352:376	effective tissue function	352:376	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	8	11	theme	MSCs	1230:1233	arg1	viability					1235:1243	MSCs viability	1230:1243	MSCs viability	1230:1243	The cell-based 3D hydrogel constructs were assessed for MSCs viability by live/dead staining.					
35527137	7	12	theme	rheological	1075:1085	arg1	properties					1102:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	15	13	theme	hydrogel	2295:2302	arg1	constructs					2304:2313	the engineered cellular 3D hydrogel constructs	2268:2313	the engineered cellular 3D hydrogel constructs	2268:2313	Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration.					
35527137	4	14	theme	type	711:714	arg1	collagen					718:725	type I collagen	711:725	type I collagen (Col I)	711:733	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	4	14	theme	type	711:714	arg1	I					732:732	Col I	728:732	Col I	728:732	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	13	15	theme	angiogenic	2006:2015	arg1	profiles					2037:2044	differential inflammatory and angiogenic paracrine secretory profiles	1976:2044	differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu	1976:2098	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	7	16	theme	Differential	1051:1062	arg1	properties					1102:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	15	17	theme	cellular	2283:2290	arg1	constructs					2304:2313	the engineered cellular 3D hydrogel constructs	2268:2313	the engineered cellular 3D hydrogel constructs	2268:2313	Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration.					
35527137	9	18	theme	flow	1291:1294	arg1	cytometry					1296:1304	flow cytometry	1291:1304	flow cytometry	1291:1304	Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs.					
35527137	12	19	theme	tuning	1830:1835	arg1	morphology					1852:1861	tuning the human MSCs morphology	1830:1861	tuning the human MSCs morphology	1830:1861	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	13	20	theme	paracrine	2017:2025	arg1	profiles					2037:2044	differential inflammatory and angiogenic paracrine secretory profiles	1976:2044	differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu	1976:2098	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	5	21	theme	GGMA	922:925	arg1	hydrogel					927:934	2) GGMA hydrogel	919:934	2) GGMA hydrogel	919:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	21	theme	GGMA	922:925	arg1	layers					885:890	two dissimilar layers	870:890	two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel	870:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	13	22	dep	engineered	2070:2079	arg1	biophysical					2081:2091	biophysical	2081:2091	biophysical	2081:2091	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	13	22	dep	engineered	2070:2079	arg1	dissimilar					2059:2068	dissimilar	2059:2068	dissimilar	2059:2068	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	11	23	theme	low	1538:1540	arg1	MSCs					1533:1536	Human MSCs	1527:1536	Human MSCs low proliferative rate and low ECM deposition	1527:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	23	theme	low	1538:1540	arg1	rate					1556:1559	low proliferative rate	1538:1559	low proliferative rate	1538:1559	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	10	24	theme	cellular	1490:1497	arg1	interface					1516:1524	a successful cellular tissue-to-tissue interface	1477:1524	a successful cellular tissue-to-tissue interface	1477:1524	A homogeneous distribution was achieved in a successful cellular tissue-to-tissue interface.					
35527137	4	25	theme	hyaluronic	739:748	arg1	HA					756:757	HA	756:757	HA	756:757	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	4	25	theme	hyaluronic	739:748	arg1	acid					750:753	hyaluronic acid	739:753	hyaluronic acid (HA)	739:758	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	13	26	theme	differential	1976:1987	arg1	profiles					2037:2044	differential inflammatory and angiogenic paracrine secretory profiles	1976:2044	differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu	1976:2098	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	0	27	theme	stem	84:87	arg1	interface					111:119	human stem cell tissue-to-tissue interface	78:119	human stem cell tissue-to-tissue interface	78:119	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	1	28	theme	functional	180:189	arg1	assembly					191:198	their functional assembly	174:198	their functional assembly into complex tissues	174:219	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	12	29	theme	human	1766:1770	arg1	phenotype					1777:1785	the human MSCs phenotype	1762:1785	the human MSCs phenotype	1762:1785	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	6	30	theme	mesenchymal	997:1007	arg1	MSCs					1021:1024	MSCs	1021:1024	MSCs	1021:1024	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	6	30	theme	mesenchymal	997:1007	arg1	cells					1014:1018	human mesenchymal stem cells	991:1018	human mesenchymal stem cells (MSCs)	991:1025	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	4	31	theme	methacrylated	797:809	arg1	GGMA					823:826	GGMA	823:826	GGMA	823:826	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	4	31	theme	methacrylated	797:809	arg1	gellan-gum					811:820	methacrylated gellan-gum	797:820	methacrylated gellan-gum (GGMA)	797:827	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	11	32	theme	low	1565:1567	arg1	MSCs					1533:1536	Human MSCs	1527:1536	Human MSCs low proliferative rate and low ECM deposition	1527:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	32	theme	low	1565:1567	arg1	deposition					1573:1582	low ECM deposition	1565:1582	low ECM deposition	1565:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	0	33	theme	tissue-to-tissue	94:109	arg1	interface					111:119	human stem cell tissue-to-tissue interface	78:119	human stem cell tissue-to-tissue interface	78:119	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	15	34	theme	engineered	2272:2281	arg1	constructs					2304:2313	the engineered cellular 3D hydrogel constructs	2268:2313	the engineered cellular 3D hydrogel constructs	2268:2313	Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration.					
35527137	1	35	theme	multiple	153:160	arg1	tissues					162:168	multiple tissues	153:168	multiple tissues	153:168	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	13	36	theme	cell-based	1938:1947	arg1	construct					1958:1966	the 3D cell-based hydrogel construct	1931:1966	the 3D cell-based hydrogel construct	1931:1966	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	9	37	theme	hydrogel	1345:1352	arg1	constructs					1354:1363	the 3D hydrogel constructs	1338:1363	the 3D hydrogel constructs	1338:1363	Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs.					
35527137	14	38	theme	nearby	2122:2127	arg1	environment					2144:2154	the nearby macromolecular environment	2118:2154	the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate	2118:2231	Human MSCs sense the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate.					
35527137	12	39	theme	hydrogel	1731:1738	arg1	constructs					1740:1749	The 3D hydrogel constructs	1724:1749	The 3D hydrogel constructs	1724:1749	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	13	40	theme	construct	1958:1966	arg1	properties					1917:1926	The intrinsic properties	1903:1926	The intrinsic properties of the 3D cell-based hydrogel construct	1903:1966	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	3	41	theme	macromolecular	486:499	arg1	composition					501:511	the macromolecular composition	482:511	the macromolecular composition of a 3D hydrogel construct	482:538	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	5	42	theme	hydrogel	837:844	arg1	constructs					846:855	The 3D hydrogel constructs	830:855	The 3D hydrogel constructs	830:855	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	3	43	theme	functional	430:439	arg1	property					441:448	differential functional property	417:448	differential functional property	417:448	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	11	44	theme	Human	1527:1531	arg1	MSCs					1533:1536	Human MSCs	1527:1536	Human MSCs low proliferative rate and low ECM deposition	1527:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	44	theme	Human	1527:1531	arg1	rate					1556:1559	low proliferative rate	1538:1559	low proliferative rate	1538:1559	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	44	theme	Human	1527:1531	arg1	deposition					1573:1582	low ECM deposition	1565:1582	low ECM deposition	1565:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	3	45	theme	construct	530:538	arg1	composition					501:511	the macromolecular composition	482:511	the macromolecular composition of a 3D hydrogel construct	482:538	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	9	46	theme	cell	1380:1383	arg1	viability					1385:1393	cell viability	1380:1393	cell viability	1380:1393	Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs.					
35527137	14	47	theme	Human	2101:2105	arg1	MSCs					2107:2110	Human MSCs	2101:2110	Human MSCs	2101:2110	Human MSCs sense the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate.					
35527137	4	48	theme	macromolecular	628:641	arg1	constructs					643:652	macromolecular constructs	628:652	macromolecular constructs	628:652	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	3	49	theme	3D	518:519	arg1	construct					530:538	a 3D hydrogel construct	516:538	a 3D hydrogel construct	516:538	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	0	50	theme	3D	31:32	arg1	construct					43:51	a 3D hydrogel construct	29:51	a 3D hydrogel construct	29:51	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	3	51	dep	worked	393:398	arg1	hypothesize					400:410	hypothesize	400:410	worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate	393:577	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	3	52	theme	stem	564:567	arg1	fate					574:577	stem cell fate	564:577	stem cell fate	564:577	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	0	53	theme	construct	43:51	arg1	modulation					15:24	Macromolecular modulation	0:24	Macromolecular modulation of a 3D hydrogel construct	0:51	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	9	54	theme	apoptosis	1403:1411	arg1	induction					1413:1421	no apoptosis induction	1400:1421	no apoptosis induction onto MSCs	1400:1431	Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs.					
35527137	14	55	theme	cell-ECM	2170:2177	arg1	interactions					2179:2190	the cell-ECM interactions	2166:2190	the cell-ECM interactions	2166:2190	Human MSCs sense the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate.					
35527137	11	56	theme	proliferative	1629:1641	arg1	rate					1643:1646	lower proliferative rate	1623:1646	lower proliferative rate within the ECM microenvironment	1623:1678	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	15	57	theme	immune	2342:2347	arg1	regeneration					2349:2360	immune regeneration	2342:2360	immune regeneration	2342:2360	Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration.					
35527137	4	58	link	natural-derived	765:779	arg1	biomaterials					781:792	natural-derived biomaterials	765:792	natural-derived biomaterials	765:792	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	11	59	theme	MSCs	1718:1721	arg1	self-renewal					1702:1713	self-renewal	1702:1713	self-renewal	1702:1713	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	7	60	theme	3D	1141:1142	arg1	constructs					1162:1171	the acellular 3D gradient hydrogel constructs	1127:1171	the acellular 3D gradient hydrogel constructs	1127:1171	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	11	61	theme	ECM	1659:1661	arg1	microenvironment					1663:1678	the ECM microenvironment	1655:1678	the ECM microenvironment	1655:1678	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	8	62	theme	cell-based	1178:1187	arg1	constructs					1201:1210	The cell-based 3D hydrogel constructs	1174:1210	The cell-based 3D hydrogel constructs	1174:1210	The cell-based 3D hydrogel constructs were assessed for MSCs viability by live/dead staining.					
35527137	12	63	theme	MSCs	1847:1850	arg1	morphology					1852:1861	tuning the human MSCs morphology	1830:1861	tuning the human MSCs morphology	1830:1861	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	5	64	dep	hydrogel	927:934	arg1	2					919:919	2	919:919	2	919:919	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	2	65	theme	adjacent	323:330	arg1	tissues					332:338	two adjacent tissues	319:338	two adjacent tissues	319:338	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	8	66	theme	hydrogel	1192:1199	arg1	constructs					1201:1210	The cell-based 3D hydrogel constructs	1174:1210	The cell-based 3D hydrogel constructs	1174:1210	The cell-based 3D hydrogel constructs were assessed for MSCs viability by live/dead staining.					
35527137	12	67	theme	macromolecular	1796:1809	arg1	modulation					1811:1820	the macromolecular modulation	1792:1820	the macromolecular modulation	1792:1820	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	4	68	theme	I	716:716	arg1	collagen					718:725	type I collagen	711:725	type I collagen (Col I)	711:733	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	4	68	theme	I	716:716	arg1	I					732:732	Col I	728:732	Col I	728:732	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	2	69	theme	tissue	362:367	arg1	function					369:376	effective tissue function	352:376	effective tissue function	352:376	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	1	70	theme	complex	205:211	arg1	tissues					213:219	complex tissues	205:219	complex tissues	205:219	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	7	71	theme	functional	1064:1073	arg1	properties					1102:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties	1051:1111	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	10	72	theme	homogeneous	1436:1446	arg1	distribution					1448:1459	A homogeneous distribution	1434:1459	A homogeneous distribution	1434:1459	A homogeneous distribution was achieved in a successful cellular tissue-to-tissue interface.					
35527137	15	73	theme	3D	2292:2293	arg1	constructs					2304:2313	the engineered cellular 3D hydrogel constructs	2268:2313	the engineered cellular 3D hydrogel constructs	2268:2313	Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration.					
35527137	4	74	theme	-based	693:698	arg1	materials					700:708	-based materials	693:708	-based materials	693:708	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	5	75	theme	dissimilar	874:883	arg1	hydrogel					906:913	HA hydrogel	903:913	HA hydrogel	903:913	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	75	theme	dissimilar	874:883	arg1	hydrogel					927:934	2) GGMA hydrogel	919:934	2) GGMA hydrogel	919:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	75	theme	dissimilar	874:883	arg1	layers					885:890	two dissimilar layers	870:890	two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel	870:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	6	76	theme	tissue-to-tissue	941:956	arg1	interface					958:966	The tissue-to-tissue interface	937:966	The tissue-to-tissue interface	937:966	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	1	77	theme	simultaneous	126:137	arg1	generation					139:148	The simultaneous generation	122:148	The simultaneous generation of multiple tissues	122:168	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	8	78	theme	live/dead	1248:1256	arg1	staining					1258:1265	live/dead staining	1248:1265	live/dead staining	1248:1265	The cell-based 3D hydrogel constructs were assessed for MSCs viability by live/dead staining.					
35527137	11	79	theme	proliferative	1542:1554	arg1	MSCs					1533:1536	Human MSCs	1527:1536	Human MSCs low proliferative rate and low ECM deposition	1527:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	79	theme	proliferative	1542:1554	arg1	rate					1556:1559	low proliferative rate	1538:1559	low proliferative rate	1538:1559	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	12	80	theme	MSCs	1772:1775	arg1	phenotype					1777:1785	the human MSCs phenotype	1762:1785	the human MSCs phenotype	1762:1785	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	13	81	theme	secretory	2027:2035	arg1	profiles					2037:2044	differential inflammatory and angiogenic paracrine secretory profiles	1976:2044	differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu	1976:2098	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	0	82	theme	human	78:82	arg1	interface					111:119	human stem cell tissue-to-tissue interface	78:119	human stem cell tissue-to-tissue interface	78:119	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	4	83	dep	matrix	681:686	arg1	materials					700:708	-based materials	693:708	-based materials	693:708	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	2	84	theme	tissue-to-tissue	281:296	arg1	interface					298:306	The tissue-to-tissue interface	277:306	The tissue-to-tissue interface connecting two adjacent tissues	277:338	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	2	84	theme	tissue-to-tissue	281:296	arg1	vital					343:347	vital	343:347	vital	343:347	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	1	85	theme	tissues	162:168	arg1	assembly					191:198	their functional assembly	174:198	their functional assembly into complex tissues	174:219	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	1	85	theme	tissues	162:168	arg1	generation					139:148	The simultaneous generation	122:148	The simultaneous generation of multiple tissues	122:168	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	11	86	theme	ECM	1569:1571	arg1	MSCs					1533:1536	Human MSCs	1527:1536	Human MSCs low proliferative rate and low ECM deposition	1527:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	86	theme	ECM	1569:1571	arg1	deposition					1573:1582	low ECM deposition	1565:1582	low ECM deposition	1565:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	0	87	theme	cell	89:92	arg1	interface					111:119	human stem cell tissue-to-tissue interface	78:119	human stem cell tissue-to-tissue interface	78:119	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	5	88	theme	HA	903:904	arg1	layers					885:890	two dissimilar layers	870:890	two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel	870:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	88	theme	HA	903:904	arg1	hydrogel					906:913	HA hydrogel	903:913	HA hydrogel	903:913	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	2	89	from	vital	343:347	arg1	function					369:376	effective tissue function	352:376	effective tissue function	352:376	The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function.					
35527137	6	90	theme	stem	1009:1012	arg1	MSCs					1021:1024	MSCs	1021:1024	MSCs	1021:1024	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	6	90	theme	stem	1009:1012	arg1	cells					1014:1018	human mesenchymal stem cells	991:1018	human mesenchymal stem cells (MSCs)	991:1025	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	10	91	theme	successful	1479:1488	arg1	interface					1516:1524	a successful cellular tissue-to-tissue interface	1477:1524	a successful cellular tissue-to-tissue interface	1477:1524	A homogeneous distribution was achieved in a successful cellular tissue-to-tissue interface.					
35527137	11	92	dep	MSCs	1533:1536	arg1	MSCs					1533:1536	Human MSCs	1527:1536	Human MSCs low proliferative rate and low ECM deposition	1527:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	92	dep	MSCs	1533:1536	arg1	rate					1556:1559	low proliferative rate	1538:1559	low proliferative rate	1538:1559	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	11	92	dep	MSCs	1533:1536	arg1	deposition					1573:1582	low ECM deposition	1565:1582	low ECM deposition	1565:1582	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	13	93	theme	inflammatory	1989:2000	arg1	profiles					2037:2044	differential inflammatory and angiogenic paracrine secretory profiles	1976:2044	differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu	1976:2098	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	6	94	theme	human	991:995	arg1	MSCs					1021:1024	MSCs	1021:1024	MSCs	1021:1024	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	6	94	theme	human	991:995	arg1	cells					1014:1018	human mesenchymal stem cells	991:1018	human mesenchymal stem cells (MSCs)	991:1025	The tissue-to-tissue interface was created by seeding human mesenchymal stem cells (MSCs) between the two layers.					
35527137	3	95	theme	differential	417:428	arg1	property					441:448	differential functional property	417:448	differential functional property	417:448	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	13	96	theme	3D	1935:1936	arg1	construct					1958:1966	the 3D cell-based hydrogel construct	1931:1966	the 3D cell-based hydrogel construct	1931:1966	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	9	97	theme	3D	1342:1343	arg1	constructs					1354:1363	the 3D hydrogel constructs	1338:1363	the 3D hydrogel constructs	1338:1363	Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs.					
35527137	14	98	theme	macromolecular	2129:2142	arg1	environment					2144:2154	the nearby macromolecular environment	2118:2154	the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate	2118:2231	Human MSCs sense the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate.					
35527137	13	99	theme	hydrogel	1949:1956	arg1	construct					1958:1966	the 3D cell-based hydrogel construct	1931:1966	the 3D cell-based hydrogel construct	1931:1966	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	5	100	theme	3D	834:835	arg1	constructs					846:855	The 3D hydrogel constructs	830:855	The 3D hydrogel constructs	830:855	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	9	101	theme	constructs	1354:1363	arg1	feasibility					1323:1333	the feasibility	1319:1333	the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs	1319:1431	Assessing apoptosis by flow cytometry, data showed the feasibility of the 3D hydrogel constructs in maintaining cell viability with no apoptosis induction onto MSCs.					
35527137	12	102	theme	3D	1728:1729	arg1	constructs					1740:1749	The 3D hydrogel constructs	1724:1749	The 3D hydrogel constructs	1724:1749	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	10	103	theme	tissue-to-tissue	1499:1514	arg1	interface					1516:1524	a successful cellular tissue-to-tissue interface	1477:1524	a successful cellular tissue-to-tissue interface	1477:1524	A homogeneous distribution was achieved in a successful cellular tissue-to-tissue interface.					
35527137	13	104	theme	intrinsic	1907:1915	arg1	properties					1917:1926	The intrinsic properties	1903:1926	The intrinsic properties of the 3D cell-based hydrogel construct	1903:1966	The intrinsic properties of the 3D cell-based hydrogel construct induced differential inflammatory and angiogenic paracrine secretory profiles owing to the dissimilar engineered biophysical milieu.					
35527137	14	105	theme	cell	2209:2212	arg1	behaviour					2214:2222	cell behaviour	2209:2222	cell behaviour	2209:2222	Human MSCs sense the nearby macromolecular environment adjusting the cell-ECM interactions, which influence cell behaviour and fate.					
35527137	4	106	theme	natural-derived	765:779	arg1	biomaterials					781:792	natural-derived biomaterials	765:792	natural-derived biomaterials	765:792	Hence, it was possible to successfully generate macromolecular constructs by using the extracellular matrix (ECM)-based materials; type I collagen (Col I) and hyaluronic acid (HA); and natural-derived biomaterials as methacrylated gellan-gum (GGMA).					
35527137	0	107	theme	hydrogel	34:41	arg1	construct					43:51	a 3D hydrogel construct	29:51	a 3D hydrogel construct	29:51	Macromolecular modulation of a 3D hydrogel construct differentially regulates human stem cell tissue-to-tissue interface.					
35527137	1	108	theme	critical	231:238	arg1	challenge					240:248	a critical challenge	229:248	a critical challenge for regenerative medicine	229:274	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
35527137	5	109	dep	Col	896:898	arg1	1					893:893	1	893:893	1	893:893	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	3	110	theme	hydrogel	521:528	arg1	construct					530:538	a 3D hydrogel construct	516:538	a 3D hydrogel construct	516:538	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	7	111	theme	hydrogel	1153:1160	arg1	constructs					1162:1171	the acellular 3D gradient hydrogel constructs	1127:1171	the acellular 3D gradient hydrogel constructs	1127:1171	Differential functional rheological and mechanical properties characterized the acellular 3D gradient hydrogel constructs.					
35527137	15	112	theme	multi-tissue	2241:2252	arg1	regeneration					2254:2265	multi-tissue regeneration	2241:2265	multi-tissue regeneration	2241:2265	Beyond multi-tissue regeneration, the engineered cellular 3D hydrogel constructs may simultaneously address immune regeneration.					
35527137	12	113	theme	spindle-shaped	1877:1890	arg1	phenotype					1892:1900	spindle-shaped phenotype	1877:1900	spindle-shaped phenotype	1877:1900	The 3D hydrogel constructs maintained the human MSCs phenotype, yet the macromolecular modulation allowed tuning the human MSCs morphology from round to spindle-shaped phenotype.					
35527137	11	114	theme	lower	1623:1627	arg1	rate					1643:1646	lower proliferative rate	1623:1646	lower proliferative rate within the ECM microenvironment	1623:1678	Human MSCs low proliferative rate and low ECM deposition were seen for all constructs; however, lower proliferative rate within the ECM microenvironment highlights controlled self-renewal of MSCs.					
35527137	3	115	theme	cell	569:572	arg1	fate					574:577	stem cell fate	564:577	stem cell fate	564:577	The presented worked hypothesize that differential functional property can be engineered by modulating the macromolecular composition of a 3D hydrogel construct and distinctively endow stem cell fate.					
35527137	5	116	dep	layers	885:890	arg1	layers					885:890	two dissimilar layers	870:890	two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel	870:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	116	dep	layers	885:890	arg1	hydrogel					927:934	2) GGMA hydrogel	919:934	2) GGMA hydrogel	919:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	116	dep	layers	885:890	arg1	hydrogel					906:913	HA hydrogel	903:913	HA hydrogel	903:913	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	5	116	dep	layers	885:890	arg1	Col					896:898	Col I	896:900	two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel	870:934	The 3D hydrogel constructs consisted of two dissimilar layers: 1) Col I: HA hydrogel and 2) GGMA hydrogel.					
35527137	1	117	theme	regenerative	254:265	arg1	medicine					267:274	regenerative medicine	254:274	regenerative medicine	254:274	The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine.					
33811551	1	0	theme	chitosan	220:227	arg1	extract					229:235	chitosan extract	220:235	chitosan extract	220:235	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	12	1	theme	economic	1855:1862	arg1	efficiency					1864:1873	economic efficiency	1855:1873	economic efficiency	1855:1873	In summary, supplementing extracted chitosan showed more potential than did the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste.					
33811551	4	2	theme	different	755:763	arg1	sources					765:771	different sources	755:771	different sources of chitosan	755:783	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	7	3	theme	different	1002:1010	arg1	sources					1012:1018	The different sources	998:1018	The different sources of chitosan supplements	998:1042	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	3	4	dep	control	490:496	arg1	addition					478:485	addition	478:485	addition	478:485	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	12	5	theme	commercial	1821:1830	arg1	chitosan					1832:1839	the commercial chitosan	1817:1839	the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste	1817:1944	In summary, supplementing extracted chitosan showed more potential than did the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste.					
33811551	2	6	theme	square	460:465	arg1	design					467:472	a 3 × 3 replicated Latin square design	435:472	a 3 × 3 replicated Latin square design	435:472	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	0	7	theme	tropical	99:106	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	1	8	theme	dairy	327:331	arg1	cows					333:336	tropical lactating dairy cows	308:336	tropical lactating dairy cows	308:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	2	9	theme	replicated	443:452	arg1	design					467:472	a 3 × 3 replicated Latin square design	435:472	a 3 × 3 replicated Latin square design	435:472	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	0	10	from	fermentation	62:73	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	0	11	theme	dairy	118:122	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	1	12	from	production	294:303	arg1	cows					333:336	tropical lactating dairy cows	308:336	tropical lactating dairy cows	308:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	1	13	theme	chitosan	179:186	arg1	sources					188:194	chitosan sources	179:194	chitosan sources (commercial chitosan vs chitosan extract)	179:236	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	11	14	theme	urea	1668:1671	arg1	nitrogen					1673:1680	milk urea nitrogen	1663:1680	milk urea nitrogen	1663:1680	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	12	15	theme	environmental	1926:1938	arg1	waste					1940:1944	environmental waste	1926:1944	environmental waste	1926:1944	In summary, supplementing extracted chitosan showed more potential than did the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste.					
33811551	0	16	from	production	85:94	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	3	17	theme	extract	513:519	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	17	theme	extract	513:519	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	17	theme	extract	513:519	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	6	18	theme	ammonia	917:923	arg1	nitrogen					925:932	ammonia nitrogen	917:932	ammonia nitrogen	917:932	The pH, temperature, ammonia nitrogen, blood urea, and microbial count were similar among treatments.					
33811551	11	19	theme	significant	1595:1605	arg1	differences					1607:1617	No significant differences	1592:1617	No significant differences	1592:1617	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	7	20	theme	butyrate	1108:1115	arg1	totals					1063:1068	the totals	1059:1068	the totals of volatile fatty acids, acetate, and butyrate	1059:1115	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	3	21	theme	%	502:502	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	21	theme	%	502:502	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	21	theme	%	502:502	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	12	22	dep	did	1813:1815	arg1	than					1808:1811	than	1808:1811	than	1808:1811	In summary, supplementing extracted chitosan showed more potential than did the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste.					
33811551	8	23	theme	ruminal	1202:1208	arg1	ratio					1232:1236	The ruminal acetate to propionate ratio	1198:1236	The ruminal acetate to propionate ratio	1198:1236	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	2	24	theme	×	439:439	arg1	design					467:472	a 3 × 3 replicated Latin square design	435:472	a 3 × 3 replicated Latin square design	435:472	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	0	25	from	Influence	0:8	arg1	fermentation					62:73	rumen fermentation	56:73	rumen fermentation	56:73	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	0	25	from	Influence	0:8	arg1	intake					33:38	intake	33:38	intake	33:38	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	0	25	from	Influence	0:8	arg1	production					85:94	milk production	80:94	milk production	80:94	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	0	25	from	Influence	0:8	arg1	digestibility					41:53	digestibility	41:53	digestibility	41:53	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	4	26	theme	significant	642:652	arg1	differences					654:664	no significant differences	639:664	no significant differences on daily dry matter, nutrients, or estimated energy intake	639:723	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	9	27	theme	supplementation	1445:1459	arg1	addition					1424:1431	the addition	1420:1431	the addition of chitosan supplementation	1420:1459	At 4 h after feeding, the methane estimation significantly decreased with the addition of chitosan supplementation (P<0.05) compared to the control group.					
33811551	11	28	theme	milk	1630:1633	arg1	yield					1635:1639	milk yield	1630:1639	milk yield	1630:1639	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	5	29	theme	commercial	863:872	arg1	supplements					883:893	extraction-based or commercial chitosan supplements	843:893	extraction-based or commercial chitosan supplements	843:893	Nutrient digestibility was not influenced differently by extraction-based or commercial chitosan supplements.					
33811551	7	30	theme	fatty	1082:1086	arg1	acids					1088:1092	volatile fatty acids	1073:1092	volatile fatty acids	1073:1092	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	1	31	theme	tropical	308:315	arg1	cows					333:336	tropical lactating dairy cows	308:336	tropical lactating dairy cows	308:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	7	32	dep	influenced	1158:1167	arg1	P<0.05					1170:1175	P<0.05	1170:1175	P<0.05	1170:1175	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	0	33	theme	chitosan	13:20	arg1	sources					22:28	chitosan sources	13:28	chitosan sources	13:28	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	5	34	theme	extraction-based	843:858	arg1	supplements					883:893	extraction-based or commercial chitosan supplements	843:893	extraction-based or commercial chitosan supplements	843:893	Nutrient digestibility was not influenced differently by extraction-based or commercial chitosan supplements.					
33811551	7	35	theme	propionate	1178:1187	arg1	content					1189:1195	propionate content	1178:1195	propionate content	1178:1195	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	3	36	theme	2	538:538	arg1	%					539:539	%	539:539	%	539:539	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	2	37	theme	Holstein-Friesian	353:369	arg1	crossbreeds					371:381	Six lactating Holstein-Friesian crossbreeds	339:381	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk)	339:416	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	1	38	theme	methane	261:267	arg1	emission					275:282	methane (CH4) emission	261:282	methane (CH4) emission	261:282	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	8	39	theme	chitosan	1273:1280	arg1	supplement					1282:1291	chitosan supplement	1273:1291	chitosan supplement	1273:1291	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	7	40	theme	supplements	1032:1042	arg1	sources					1012:1018	The different sources	998:1018	The different sources of chitosan supplements	998:1042	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	1	41	theme	CH4	270:272	arg1	emission					275:282	methane (CH4) emission	261:282	methane (CH4) emission	261:282	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	4	42	theme	dry	675:677	arg1	matter					679:684	daily dry matter	669:684	daily dry matter	669:684	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	1	43	theme	sources	188:194	arg1	influence					166:174	the influence	162:174	the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows	162:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	7	44	theme	chitosan	1141:1148	arg1	sources					1150:1156	different chitosan sources	1131:1156	different chitosan sources	1131:1156	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	3	45	theme	commercial	541:550	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	45	theme	commercial	541:550	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	45	theme	commercial	541:550	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	1	46	theme	commercial	197:206	arg1	chitosan					208:215	commercial chitosan	197:215	commercial chitosan	197:215	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	7	47	theme	chitosan	1023:1030	arg1	supplements					1032:1042	chitosan supplements	1023:1042	chitosan supplements	1023:1042	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	3	48	theme	intake	586:591	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	48	theme	intake	586:591	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	48	theme	intake	586:591	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	11	49	theme	chitosan	1722:1729	arg1	sources					1711:1717	different sources	1701:1717	different sources of chitosan (P>0.05)	1701:1738	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	3	50	theme	dry	575:577	arg1	intake					586:591	dry matter intake	575:591	dry matter intake	575:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	4	51	theme	estimated	701:709	arg1	intake					718:723	estimated energy intake	701:723	estimated energy intake	701:723	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	9	52	theme	methane	1372:1378	arg1	estimation					1380:1389	the methane estimation	1368:1389	the methane estimation	1368:1389	At 4 h after feeding, the methane estimation significantly decreased with the addition of chitosan supplementation (P<0.05) compared to the control group.					
33811551	1	53	from	fermentation	247:258	arg1	cows					333:336	tropical lactating dairy cows	308:336	tropical lactating dairy cows	308:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	1	54	theme	study	141:145	arg1	aim					134:136	The aim	130:136	The aim of study	130:145	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	10	55	theme	microbial	1528:1536	arg1	synthesis					1546:1554	microbial protein synthesis	1528:1554	microbial protein synthesis	1528:1554	The purine derivatives and microbial protein synthesis were not altered by the treatments.					
33811551	4	56	theme	energy	711:716	arg1	intake					718:723	estimated energy intake	701:723	estimated energy intake	701:723	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	11	57	theme	milk	1663:1666	arg1	nitrogen					1673:1680	milk urea nitrogen	1663:1680	milk urea nitrogen	1663:1680	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	0	58	theme	lactating	108:116	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	8	59	dep	ratio	1232:1236	arg1	to					1218:1219	to	1218:1219	to	1218:1219	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	2	60	theme	Latin	454:458	arg1	design					467:472	a 3 × 3 replicated Latin square design	435:472	a 3 × 3 replicated Latin square design	435:472	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	1	61	dep	sources	188:194	arg1	chitosan					208:215	commercial chitosan	197:215	commercial chitosan	197:215	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	1	61	dep	sources	188:194	arg1	extract					229:235	chitosan extract	220:235	chitosan extract	220:235	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	7	62	theme	acids	1088:1092	arg1	totals					1063:1068	the totals	1059:1068	the totals of volatile fatty acids, acetate, and butyrate	1059:1115	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	5	63	theme	Nutrient	786:793	arg1	digestibility					795:807	Nutrient digestibility	786:807	Nutrient digestibility	786:807	Nutrient digestibility was not influenced differently by extraction-based or commercial chitosan supplements.					
33811551	8	64	theme	chitosan	1336:1343	arg1	sources					1325:1331	sources	1325:1331	sources of chitosan	1325:1343	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	3	65	theme	chitosan	504:511	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	65	theme	chitosan	504:511	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	65	theme	chitosan	504:511	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	1	66	from	influence	166:174	arg1	fermentation					247:258	rumen fermentation	241:258	rumen fermentation	241:258	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	1	66	from	influence	166:174	arg1	emission					275:282	methane (CH4) emission	261:282	methane (CH4) emission	261:282	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	1	66	from	influence	166:174	arg1	production					294:303	milk production	289:303	milk production in tropical lactating dairy cows	289:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	4	67	theme	chitosan	776:783	arg1	sources					765:771	different sources	755:771	different sources of chitosan	755:783	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	9	68	dep	decreased	1405:1413	arg1	P<0.05					1462:1467	P<0.05	1462:1467	P<0.05	1462:1467	At 4 h after feeding, the methane estimation significantly decreased with the addition of chitosan supplementation (P<0.05) compared to the control group.					
33811551	0	69	theme	rumen	56:60	arg1	fermentation					62:73	rumen fermentation	56:73	rumen fermentation	56:73	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	3	70	theme	2	501:501	arg1	%					502:502	%	502:502	%	502:502	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	7	71	theme	acetate	1095:1101	arg1	totals					1063:1068	the totals	1059:1068	the totals of volatile fatty acids, acetate, and butyrate	1059:1115	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	12	72	theme	extracted	1767:1775	arg1	chitosan					1777:1784	extracted chitosan	1767:1784	extracted chitosan	1767:1784	In summary, supplementing extracted chitosan showed more potential than did the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste.					
33811551	8	73	dep	reduced	1260:1266	arg1	P<0.05					1252:1257	P<0.05	1252:1257	P<0.05	1252:1257	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	11	74	theme	milk	1642:1645	arg1	composition					1647:1657	milk composition	1642:1657	milk composition	1642:1657	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	0	75	theme	milk	80:83	arg1	production					85:94	milk production	80:94	milk production	80:94	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	1	76	theme	milk	289:292	arg1	production					294:303	milk production	289:303	milk production in tropical lactating dairy cows	289:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	4	77	from	differences	654:664	arg1	matter					679:684	daily dry matter	669:684	daily dry matter	669:684	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	4	77	from	differences	654:664	arg1	intake					718:723	estimated energy intake	701:723	estimated energy intake	701:723	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	4	77	from	differences	654:664	arg1	nutrients					687:695	nutrients	687:695	nutrients	687:695	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	0	78	from	digestibility	41:53	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	8	79	theme	acetate	1210:1216	arg1	ratio					1232:1236	The ruminal acetate to propionate ratio	1198:1236	The ruminal acetate to propionate ratio	1198:1236	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	7	80	theme	volatile	1073:1080	arg1	acids					1088:1092	volatile fatty acids	1073:1092	volatile fatty acids	1073:1092	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	5	81	theme	chitosan	874:881	arg1	supplements					883:893	extraction-based or commercial chitosan supplements	843:893	extraction-based or commercial chitosan supplements	843:893	Nutrient digestibility was not influenced differently by extraction-based or commercial chitosan supplements.					
33811551	0	82	from	intake	33:38	arg1	cows					124:127	tropical lactating dairy cows	99:127	tropical lactating dairy cows	99:127	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	8	83	theme	propionate	1221:1230	arg1	ratio					1232:1236	The ruminal acetate to propionate ratio	1198:1236	The ruminal acetate to propionate ratio	1198:1236	The ruminal acetate to propionate ratio was markedly (P<0.05) reduced with chitosan supplement, but no change appeared between sources of chitosan.					
33811551	10	84	theme	purine	1505:1510	arg1	derivatives					1512:1522	The purine derivatives	1501:1522	The purine derivatives	1501:1522	The purine derivatives and microbial protein synthesis were not altered by the treatments.					
33811551	1	85	theme	lactating	317:325	arg1	cows					333:336	tropical lactating dairy cows	308:336	tropical lactating dairy cows	308:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	6	86	theme	microbial	951:959	arg1	count					961:965	microbial count	951:965	microbial count	951:965	The pH, temperature, ammonia nitrogen, blood urea, and microbial count were similar among treatments.					
33811551	2	87	theme	5	390:390	arg1	±					388:388	±	388:388	±	388:388	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	0	88	theme	sources	22:28	arg1	Influence					0:8	Influence	0:8	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.	0:128	Influence of chitosan sources on intake, digestibility, rumen fermentation, and milk production in tropical lactating dairy cows.					
33811551	1	89	theme	rumen	241:245	arg1	fermentation					247:258	rumen fermentation	241:258	rumen fermentation	241:258	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	9	90	theme	chitosan	1436:1443	arg1	supplementation					1445:1459	chitosan supplementation	1436:1459	chitosan supplementation	1436:1459	At 4 h after feeding, the methane estimation significantly decreased with the addition of chitosan supplementation (P<0.05) compared to the control group.					
33811551	2	91	theme	410	384:386	arg1	±					388:388	±	388:388	±	388:388	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	10	92	theme	protein	1538:1544	arg1	synthesis					1546:1554	microbial protein synthesis	1528:1554	microbial protein synthesis	1528:1554	The purine derivatives and microbial protein synthesis were not altered by the treatments.					
33811551	6	93	theme	blood	935:939	arg1	urea					941:944	blood urea	935:944	blood urea	935:944	The pH, temperature, ammonia nitrogen, blood urea, and microbial count were similar among treatments.					
33811551	2	94	theme	±	400:400	arg1	day-in-milk					405:415	120 ± 21 day-in-milk	396:415	120 ± 21 day-in-milk	396:415	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	2	94	theme	±	400:400	arg1	kg					392:393	410 ± 5 kg	384:393	410 ± 5 kg	384:393	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	2	95	theme	lactating	343:351	arg1	crossbreeds					371:381	Six lactating Holstein-Friesian crossbreeds	339:381	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk)	339:416	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	1	96	from	emission	275:282	arg1	cows					333:336	tropical lactating dairy cows	308:336	tropical lactating dairy cows	308:336	The aim of study was to compare the influence of chitosan sources (commercial chitosan vs chitosan extract) on rumen fermentation, methane (CH4) emission, and milk production in tropical lactating dairy cows.					
33811551	3	97	theme	chitosan	552:559	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	97	theme	chitosan	552:559	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	97	theme	chitosan	552:559	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	2	98	dep	crossbreeds	371:381	arg1	day-in-milk					405:415	120 ± 21 day-in-milk	396:415	120 ± 21 day-in-milk	396:415	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	2	98	dep	crossbreeds	371:381	arg1	kg					392:393	410 ± 5 kg	384:393	410 ± 5 kg	384:393	Six lactating Holstein-Friesian crossbreeds (410 ± 5 kg, 120 ± 21 day-in-milk) were arranged in a 3 × 3 replicated Latin square design.					
33811551	7	99	theme	different	1131:1139	arg1	sources					1150:1156	different chitosan sources	1131:1156	different chitosan sources	1131:1156	The different sources of chitosan supplements did not change the totals of volatile fatty acids, acetate, and butyrate; in contrast, different chitosan sources influenced (P<0.05) propionate content.					
33811551	3	100	theme	%	539:539	arg1	supplement					521:530	a 2% chitosan extract supplement	499:530	a 2% chitosan extract supplement	499:530	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	100	theme	%	539:539	arg1	treatments					602:611	the treatments	598:611	the treatments	598:611	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	3	100	theme	%	539:539	arg1	supplement					561:570	a 2% commercial chitosan supplement	536:570	a 2% commercial chitosan supplement of dry matter intake	536:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33811551	4	101	theme	daily	669:673	arg1	matter					679:684	daily dry matter	669:684	daily dry matter	669:684	The results denoted that no significant differences on daily dry matter, nutrients, or estimated energy intake were noted when cows received different sources of chitosan.					
33811551	11	102	theme	different	1701:1709	arg1	sources					1711:1717	different sources	1701:1717	different sources of chitosan (P>0.05)	1701:1738	No significant differences existed on milk yield, milk composition, or milk urea nitrogen when cows received different sources of chitosan (P>0.05).					
33811551	12	103	theme	shrimp	1889:1894	arg1	residues					1896:1903	shrimp residues	1889:1903	shrimp residues	1889:1903	In summary, supplementing extracted chitosan showed more potential than did the commercial chitosan for enhancing economic efficiency and recycling shrimp residues, therefore, reducing environmental waste.					
33811551	9	104	theme	control	1486:1492	arg1	group					1494:1498	the control group	1482:1498	the control group	1482:1498	At 4 h after feeding, the methane estimation significantly decreased with the addition of chitosan supplementation (P<0.05) compared to the control group.					
33811551	3	105	theme	matter	579:584	arg1	intake					586:591	dry matter intake	575:591	dry matter intake	575:591	In addition to control, a 2% chitosan extract supplement and a 2% commercial chitosan supplement of dry matter intake were the treatments.					
33434469	2	0	theme	system	425:430	arg1	development					399:409	the development	395:409	the development of the immune system	395:430	Bifidobacterium, a genus that dominates the infants' gut, is adapted to breast milk in its ability to metabolize human milk oligosaccharides; it is regarded as a mutualist owing to its involvement in the development of the immune system.					
33434469	0	1	from	effect	14:19	arg1	development					55:65	the development	51:65	the development of human gut microbiota	51:89	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	2	2	theme	human	308:312	arg1	oligosaccharides					319:334	human milk oligosaccharides	308:334	human milk oligosaccharides	308:334	Bifidobacterium, a genus that dominates the infants' gut, is adapted to breast milk in its ability to metabolize human milk oligosaccharides; it is regarded as a mutualist owing to its involvement in the development of the immune system.					
33434469	10	3	dep	shaping	1567:1573	arg1	populations					1583:1593	the gut populations	1575:1593	shaping the gut populations following different birth and feeding modes	1567:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	2	4	theme	immune	418:423	arg1	system					425:430	the immune system	414:430	the immune system	414:430	Bifidobacterium, a genus that dominates the infants' gut, is adapted to breast milk in its ability to metabolize human milk oligosaccharides; it is regarded as a mutualist owing to its involvement in the development of the immune system.					
33434469	7	5	theme	immune	1117:1122	arg1	responses					1124:1132	immune responses	1117:1132	immune responses	1117:1132	Bifidobacteria associated with breast milk can trigger immune responses with lasting effects on the microbial community structure.					
33434469	9	6	theme	feeding	1456:1462	arg1	practices					1464:1472	different feeding practices	1446:1472	different feeding practices	1446:1472	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	5	7	theme	generic	843:849	arg1	compartment					851:861	a generic compartment	841:861	a generic compartment of commensal competitors	841:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	9	8	theme	microbial	1412:1420	arg1	profiles					1422:1429	microbial profiles	1412:1429	microbial profiles in response to different feeding practices	1412:1472	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	0	9	theme	gut	76:78	arg1	microbiota					80:89	human gut microbiota	70:89	human gut microbiota	70:89	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	2	10	from	involvement	380:390	arg1	development					399:409	the development	395:409	the development of the immune system	395:430	Bifidobacterium, a genus that dominates the infants' gut, is adapted to breast milk in its ability to metabolize human milk oligosaccharides; it is regarded as a mutualist owing to its involvement in the development of the immune system.					
33434469	10	11	theme	birth	1615:1619	arg1	modes					1633:1637	different birth and feeding modes	1605:1637	different birth and feeding modes	1605:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	0	12	theme	human	70:74	arg1	microbiota					80:89	human gut microbiota	70:89	human gut microbiota	70:89	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	10	13	from	importance	1504:1513	arg1	shaping					1567:1573	shaping	1567:1573	shaping the gut populations following different birth and feeding modes	1567:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	8	14	theme	post-weaning	1323:1334	arg1	profiles					1314:1321	two distinct microbial profiles	1291:1321	two distinct microbial profiles post-weaning	1291:1334	Our model shows that, in response to a change in birth environment, competition alone can produce two distinct microbial profiles post-weaning.					
33434469	4	15	theme	feeding	639:645	arg1	practices					647:655	birth and feeding practices	629:655	birth and feeding practices	629:655	However, whether and how birth and feeding practices contribute to such variation remains unclear.					
33434469	7	16	from	effects	1147:1153	arg1	structure					1182:1190	the microbial community structure	1158:1190	the microbial community structure	1158:1190	Bifidobacteria associated with breast milk can trigger immune responses with lasting effects on the microbial community structure.					
33434469	5	17	theme	commensal	866:874	arg1	competitors					876:886	commensal competitors	866:886	commensal competitors	866:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	0	18	theme	microbiota	80:89	arg1	development					55:65	the development	51:65	the development of human gut microbiota	51:89	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	7	19	theme	breast	1093:1098	arg1	milk					1100:1103	breast milk	1093:1103	breast milk	1093:1103	Bifidobacteria associated with breast milk can trigger immune responses with lasting effects on the microbial community structure.					
33434469	7	20	theme	community	1172:1180	arg1	structure					1182:1190	the microbial community structure	1158:1190	the microbial community structure	1158:1190	Bifidobacteria associated with breast milk can trigger immune responses with lasting effects on the microbial community structure.					
33434469	7	21	theme	microbial	1162:1170	arg1	structure					1182:1190	the microbial community structure	1158:1190	the microbial community structure	1158:1190	Bifidobacteria associated with breast milk can trigger immune responses with lasting effects on the microbial community structure.					
33434469	5	22	theme	early	721:725	arg1	events					727:732	early events	721:732	early events	721:732	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	8	23	dep	change	1232:1237	arg1	response					1218:1225	response	1218:1225	response	1218:1225	Our model shows that, in response to a change in birth environment, competition alone can produce two distinct microbial profiles post-weaning.					
33434469	10	24	theme	microbe-host	1538:1549	arg1	interactions					1551:1562	microbe-microbe and microbe-host interactions	1518:1562	microbe-microbe and microbe-host interactions	1518:1562	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	5	25	theme	types	813:817	arg1	model					800:804	a mathematical model	785:804	a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors	785:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	5	26	theme	microbiota	762:771	arg1	establishment					745:757	the establishment	741:757	the establishment of microbiota	741:771	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	2	27	theme	breast	267:272	arg1	milk					274:277	breast milk	267:277	breast milk	267:277	Bifidobacterium, a genus that dominates the infants' gut, is adapted to breast milk in its ability to metabolize human milk oligosaccharides; it is regarded as a mutualist owing to its involvement in the development of the immune system.					
33434469	1	28	theme	vaginal	162:168	arg1	birth					170:174	vaginal birth	162:174	vaginal birth	162:174	The human gut microbiota is transmitted from mother to infant through vaginal birth and breastfeeding.					
33434469	10	29	theme	feeding	1625:1631	arg1	modes					1633:1637	different birth and feeding modes	1605:1637	different birth and feeding modes	1605:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	9	30	from	profiles	1422:1429	arg1	response					1434:1441	response	1434:1441	response to different feeding practices	1434:1472	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	6	31	from	alterations	1023:1033	arg1	profiles					1052:1059	gut microbial profiles	1038:1059	gut microbial profiles	1038:1059	We show how early events affect competition between mutualists and commensals and microbe-host-immune interactions to cause long-term alterations in gut microbial profiles.					
33434469	3	32	theme	Bifidobacteria	491:504	arg1	abundance					478:486	the abundance	474:486	the abundance of Bifidobacteria	474:504	The composition of microbiota, including the abundance of Bifidobacteria, is highly variable between individuals and some microbial profiles are associated with diseases.					
33434469	8	33	theme	birth	1242:1246	arg1	environment					1248:1258	birth environment	1242:1258	birth environment	1242:1258	Our model shows that, in response to a change in birth environment, competition alone can produce two distinct microbial profiles post-weaning.					
33434469	8	34	theme	microbial	1304:1312	arg1	profiles					1314:1321	two distinct microbial profiles	1291:1321	two distinct microbial profiles post-weaning	1291:1334	Our model shows that, in response to a change in birth environment, competition alone can produce two distinct microbial profiles post-weaning.					
33434469	3	35	theme	microbiota	452:461	arg1	variable					517:524	variable	517:524	variable	517:524	The composition of microbiota, including the abundance of Bifidobacteria, is highly variable between individuals and some microbial profiles are associated with diseases.					
33434469	3	35	theme	microbiota	452:461	arg1	composition					437:447	The composition	433:447	The composition	433:447	The composition of microbiota, including the abundance of Bifidobacteria, is highly variable between individuals and some microbial profiles are associated with diseases.					
33434469	7	36	theme	lasting	1139:1145	arg1	effects					1147:1153	lasting effects	1139:1153	lasting effects on the microbial community structure	1139:1190	Bifidobacteria associated with breast milk can trigger immune responses with lasting effects on the microbial community structure.					
33434469	6	37	theme	early	901:905	arg1	events					907:912	early events	901:912	early events	901:912	We show how early events affect competition between mutualists and commensals and microbe-host-immune interactions to cause long-term alterations in gut microbial profiles.					
33434469	6	38	theme	long-term	1013:1021	arg1	alterations					1023:1033	long-term alterations	1013:1033	long-term alterations in gut microbial profiles	1013:1059	We show how early events affect competition between mutualists and commensals and microbe-host-immune interactions to cause long-term alterations in gut microbial profiles.					
33434469	8	39	theme	distinct	1295:1302	arg1	profiles					1314:1321	two distinct microbial profiles	1291:1321	two distinct microbial profiles post-weaning	1291:1334	Our model shows that, in response to a change in birth environment, competition alone can produce two distinct microbial profiles post-weaning.					
33434469	6	40	theme	gut	1038:1040	arg1	profiles					1052:1059	gut microbial profiles	1038:1059	gut microbial profiles	1038:1059	We show how early events affect competition between mutualists and commensals and microbe-host-immune interactions to cause long-term alterations in gut microbial profiles.					
33434469	5	41	theme	mathematical	787:798	arg1	model					800:804	a mathematical model	785:804	a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors	785:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	0	42	theme	birth	24:28	arg1	effect					14:19	the effect	10:19	the effect of birth and feeding modes on the development of human gut microbiota	10:89	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	8	43	from	change	1232:1237	arg1	environment					1248:1258	birth environment	1242:1258	birth environment	1242:1258	Our model shows that, in response to a change in birth environment, competition alone can produce two distinct microbial profiles post-weaning.					
33434469	6	44	theme	microbial	1042:1050	arg1	profiles					1052:1059	gut microbial profiles	1038:1059	gut microbial profiles	1038:1059	We show how early events affect competition between mutualists and commensals and microbe-host-immune interactions to cause long-term alterations in gut microbial profiles.					
33434469	5	45	theme	Bifidobacteria	822:835	arg1	types					813:817	two types	809:817	two types of Bifidobacteria and a generic compartment of commensal competitors	809:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	10	46	theme	interactions	1551:1562	arg1	importance					1504:1513	the importance	1500:1513	the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes	1500:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	5	47	theme	compartment	851:861	arg1	types					813:817	two types	809:817	two types of Bifidobacteria and a generic compartment of commensal competitors	809:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	0	48	theme	feeding	34:40	arg1	effect					14:19	the effect	10:19	the effect of birth and feeding modes on the development of human gut microbiota	10:89	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	9	49	from	variations	1398:1407	arg1	profiles					1422:1429	microbial profiles	1412:1429	microbial profiles in response to different feeding practices	1412:1472	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	0	50	dep	birth	24:28	arg1	modes					42:46	modes	42:46	modes	42:46	Modelling the effect of birth and feeding modes on the development of human gut microbiota.					
33434469	1	51	theme	human	96:100	arg1	microbiota					106:115	The human gut microbiota	92:115	The human gut microbiota	92:115	The human gut microbiota is transmitted from mother to infant through vaginal birth and breastfeeding.					
33434469	9	52	theme	competition	1369:1379	arg1	model					1381:1385	our competition model	1365:1385	our competition model	1365:1385	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	9	53	theme	immune	1344:1349	arg1	regulation					1351:1360	immune regulation	1344:1360	immune regulation	1344:1360	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	4	54	theme	such	671:674	arg1	variation					676:684	such variation	671:684	such variation	671:684	However, whether and how birth and feeding practices contribute to such variation remains unclear.					
33434469	10	55	theme	microbe-microbe	1518:1532	arg1	interactions					1551:1562	microbe-microbe and microbe-host interactions	1518:1562	microbe-microbe and microbe-host interactions	1518:1562	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	4	56	dep	whether	613:619	arg1	contribute					657:666	contribute	657:666	contribute to such variation	657:684	However, whether and how birth and feeding practices contribute to such variation remains unclear.					
33434469	5	57	theme	competitors	876:886	arg1	Bifidobacteria					822:835	Bifidobacteria	822:835	Bifidobacteria	822:835	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	5	57	theme	competitors	876:886	arg1	compartment					851:861	a generic compartment	841:861	a generic compartment of commensal competitors	841:886	To understand how early events affect the establishment of microbiota, we develop a mathematical model of two types of Bifidobacteria and a generic compartment of commensal competitors.					
33434469	1	58	theme	gut	102:104	arg1	microbiota					106:115	The human gut microbiota	92:115	The human gut microbiota	92:115	The human gut microbiota is transmitted from mother to infant through vaginal birth and breastfeeding.					
33434469	10	59	theme	gut	1579:1581	arg1	populations					1583:1593	the gut populations	1575:1593	shaping the gut populations following different birth and feeding modes	1567:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33434469	4	60	theme	birth	629:633	arg1	practices					647:655	birth and feeding practices	629:655	birth and feeding practices	629:655	However, whether and how birth and feeding practices contribute to such variation remains unclear.					
33434469	6	61	theme	microbe-host-immune	971:989	arg1	interactions					991:1002	microbe-host-immune interactions	971:1002	microbe-host-immune interactions	971:1002	We show how early events affect competition between mutualists and commensals and microbe-host-immune interactions to cause long-term alterations in gut microbial profiles.					
33434469	9	62	theme	different	1446:1454	arg1	practices					1464:1472	different feeding practices	1446:1472	different feeding practices	1446:1472	Adding immune regulation to our competition model allows for variations in microbial profiles in response to different feeding practices.					
33434469	3	63	theme	microbial	555:563	arg1	profiles					565:572	some microbial profiles	550:572	some microbial profiles	550:572	The composition of microbiota, including the abundance of Bifidobacteria, is highly variable between individuals and some microbial profiles are associated with diseases.					
33434469	2	64	theme	milk	314:317	arg1	oligosaccharides					319:334	human milk oligosaccharides	308:334	human milk oligosaccharides	308:334	Bifidobacterium, a genus that dominates the infants' gut, is adapted to breast milk in its ability to metabolize human milk oligosaccharides; it is regarded as a mutualist owing to its involvement in the development of the immune system.					
33434469	10	65	theme	different	1605:1613	arg1	modes					1633:1637	different birth and feeding modes	1605:1637	different birth and feeding modes	1605:1637	This analysis highlights the importance of microbe-microbe and microbe-host interactions in shaping the gut populations following different birth and feeding modes.					
33169516	0	0	theme	Myocardial	79:88	arg1	Infarction					90:99	Myocardial Infarction	79:99	Myocardial Infarction Treatment In vivo	79:117	A Reactive Oxygen Species Scavenging and O2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo.					
33169516	5	1	theme	increase	1023:1030	arg1	ratio					1049:1053	increase M2/M1 macrophage ratio	1023:1053	increase M2/M1 macrophage ratio	1023:1053	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	1	theme	increase	1023:1030	arg1	apoptosis					1012:1020	cell apoptosis	1007:1020	cell apoptosis	1007:1020	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	2	2	theme	causes	441:446	arg1	causes					441:446	the leading causes	429:446	the leading causes of death nowadays	429:464	In particular, the MI has become one of the leading causes of death nowadays.					
33169516	2	2	theme	causes	441:446	arg1	one					422:424	one	422:424	one	422:424	In particular, the MI has become one of the leading causes of death nowadays.					
33169516	5	3	theme	M2/M1	1032:1036	arg1	ratio					1049:1053	increase M2/M1 macrophage ratio	1023:1053	increase M2/M1 macrophage ratio	1023:1053	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	3	theme	M2/M1	1032:1036	arg1	apoptosis					1012:1020	cell apoptosis	1007:1020	cell apoptosis	1007:1020	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	4	theme	major	869:873	arg1	type					875:878	a major type	867:878	a major type of ROS	867:885	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	4	theme	major	869:873	arg1	H2					859:860	H2	859:860	H2	859:860	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	5	theme	macrophage	1038:1047	arg1	ratio					1049:1053	increase M2/M1 macrophage ratio	1023:1053	increase M2/M1 macrophage ratio	1023:1053	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	5	theme	macrophage	1038:1047	arg1	apoptosis					1012:1020	cell apoptosis	1007:1020	cell apoptosis	1007:1020	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	6	theme	model	922:926	arg1	Results					904:910	O2 and H2 O. Results	891:910	O2 and H2 O. Results of rat MI model	891:926	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	3	7	theme	novel	484:488	arg1	type					490:493	a novel type	482:493	a novel type of injectable hydrogel with dual functions of ROS scavenging	482:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	4	8	theme	ROS-cleavable	667:679	arg1	polymers					695:702	the synthetic ROS-cleavable hyperbranched polymers	653:702	the synthetic ROS-cleavable hyperbranched polymers	653:702	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	0	9	theme	Infarction	90:99	arg1	Treatment					101:109	Myocardial Infarction Treatment	79:109	Myocardial Infarction Treatment In vivo	79:117	A Reactive Oxygen Species Scavenging and O2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo.					
33169516	1	10	theme	severe	334:339	arg1	fibrosis					341:348	severe fibrosis	334:348	severe fibrosis	334:348	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	6	11	theme	simple	1171:1176	arg1	structure					1178:1186	simple structure	1171:1186	simple structure	1171:1186	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	5	12	theme	cardiac	1113:1119	arg1	functions					1121:1129	cardiac functions	1113:1129	cardiac functions	1113:1129	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	13	theme	biocompatible	779:791	arg1	catalase					808:815	biocompatible and applicable catalase	779:815	biocompatible and applicable catalase	779:815	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	4	14	theme	synthetic	657:665	arg1	polymers					695:702	the synthetic ROS-cleavable hyperbranched polymers	653:702	the synthetic ROS-cleavable hyperbranched polymers	653:702	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	4	15	theme	hyaluronic	721:730	arg1	HA-MA					738:742	HA-MA	738:742	HA-MA	738:742	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	4	15	theme	hyaluronic	721:730	arg1	acid					732:735	methacrylate hyaluronic acid	708:735	methacrylate hyaluronic acid (HA-MA)	708:743	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	5	16	theme	catalase	808:815	arg1	Addition					767:774	Addition	767:774	Addition of biocompatible and applicable catalase	767:815	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	6	17	theme	therapeutic	1248:1258	arg1	effect					1260:1265	very excellent MI therapeutic effect	1230:1265	very excellent MI therapeutic effect	1230:1265	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	5	18	theme	cell	1007:1010	arg1	ratio					1049:1053	increase M2/M1 macrophage ratio	1023:1053	increase M2/M1 macrophage ratio	1023:1053	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	18	theme	cell	1007:1010	arg1	apoptosis					1012:1020	cell apoptosis	1007:1020	cell apoptosis	1007:1020	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	4	19	theme	methacrylate	708:719	arg1	HA-MA					738:742	HA-MA	738:742	HA-MA	738:742	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	4	19	theme	methacrylate	708:719	arg1	acid					732:735	methacrylate hyaluronic acid	708:735	methacrylate hyaluronic acid (HA-MA)	708:743	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	0	20	theme	Reactive	2:9	arg1	Species					18:24	A Reactive Oxygen Species	0:24	A Reactive Oxygen Species Scavenging	0:35	A Reactive Oxygen Species Scavenging and O2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo.					
33169516	6	21	theme	ROS	1273:1275	arg1	scavenging					1277:1286	this ROS scavenging	1268:1286	this ROS scavenging	1268:1286	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	6	22	theme	degradation	1153:1163	arg1	rate					1165:1168	the appropriate degradation rate	1137:1168	the appropriate degradation rate	1137:1168	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	3	23	theme	hydrogel	509:516	arg1	type					490:493	a novel type	482:493	a novel type of injectable hydrogel with dual functions of ROS scavenging	482:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	6	24	theme	appropriate	1141:1151	arg1	rate					1165:1168	the appropriate degradation rate	1137:1168	the appropriate degradation rate	1137:1168	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	6	25	theme	excellent	1235:1243	arg1	effect					1260:1265	very excellent MI therapeutic effect	1230:1265	very excellent MI therapeutic effect	1230:1265	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	3	26	theme	MI	592:593	arg1	treatment					595:603	MI treatment	592:603	MI treatment	592:603	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	0	27	theme	Species	18:24	arg1	Scavenging					26:35	A Reactive Oxygen Species Scavenging	0:35	A Reactive Oxygen Species Scavenging	0:35	A Reactive Oxygen Species Scavenging and O2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo.					
33169516	6	28	theme	MI	1245:1246	arg1	effect					1260:1265	very excellent MI therapeutic effect	1230:1265	very excellent MI therapeutic effect	1230:1265	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	4	29	from	polymers	695:702	arg1	HA-MA					738:742	HA-MA	738:742	HA-MA	738:742	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	4	29	from	polymers	695:702	arg1	acid					732:735	methacrylate hyaluronic acid	708:735	methacrylate hyaluronic acid (HA-MA)	708:743	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	4	29	from	polymers	695:702	arg1	s					646:646	3 s	644:646	3 s from the synthetic ROS-cleavable hyperbranched polymers	644:702	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	4	30	theme	hyperbranched	681:693	arg1	polymers					695:702	the synthetic ROS-cleavable hyperbranched polymers	653:702	the synthetic ROS-cleavable hyperbranched polymers	653:702	The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation.					
33169516	5	31	theme	ROS	883:885	arg1	type					875:878	a major type	867:878	a major type of ROS	867:885	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	31	theme	ROS	883:885	arg1	H2					859:860	H2	859:860	H2	859:860	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	0	32	theme	Oxygen	11:16	arg1	Species					18:24	A Reactive Oxygen Species	0:24	A Reactive Oxygen Species Scavenging	0:35	A Reactive Oxygen Species Scavenging and O2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo.					
33169516	5	33	theme	infarcted	1085:1093	arg1	area					1095:1098	infarcted area	1085:1098	infarcted area	1085:1098	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	34	theme	O2	891:892	arg1	Results					904:910	O2 and H2 O. Results	891:910	O2 and H2 O. Results of rat MI model	891:926	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	3	35	theme	injectable	498:507	arg1	hydrogel					509:516	injectable hydrogel	498:516	injectable hydrogel with dual functions of ROS scavenging	498:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	1	36	theme	inflammation-related	192:211	arg1	infarction					241:250	myocardial infarction	230:250	myocardial infarction (MI)	230:255	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	36	theme	inflammation-related	192:211	arg1	diseases					213:220	the inflammation-related diseases	188:220	the inflammation-related diseases such as myocardial infarction (MI)	188:255	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	2	37	theme	leading	433:439	arg1	causes					441:446	the leading causes	429:446	the leading causes of death nowadays	429:464	In particular, the MI has become one of the leading causes of death nowadays.					
33169516	1	38	theme	normal	280:285	arg1	repair					294:299	the normal tissue repair	276:299	the normal tissue repair	276:299	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	39	theme	tissues	369:375	arg1	fibrosis					341:348	severe fibrosis	334:348	severe fibrosis	334:348	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	39	theme	tissues	369:375	arg1	malfunction					354:364	malfunction	354:364	malfunction of tissues and organs	354:386	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	5	40	theme	rat	915:917	arg1	model					922:926	rat MI model	915:926	rat MI model	915:926	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	1	41	theme	tissue	287:292	arg1	repair					294:299	the normal tissue repair	276:299	the normal tissue repair	276:299	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	5	42	theme	excessive	984:992	arg1	ROS					994:996	excessive ROS	984:996	excessive ROS	984:996	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	3	43	theme	ROS	541:543	arg1	scavenging					545:554	ROS scavenging	541:554	ROS scavenging	541:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	5	44	theme	applicable	797:806	arg1	catalase					808:815	biocompatible and applicable catalase	779:815	biocompatible and applicable catalase	779:815	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	1	45	theme	organs	381:386	arg1	fibrosis					341:348	severe fibrosis	334:348	severe fibrosis	334:348	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	45	theme	organs	381:386	arg1	malfunction					354:364	malfunction	354:364	malfunction of tissues and organs	354:386	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	3	46	theme	dual	523:526	arg1	functions					528:536	dual functions	523:536	dual functions of ROS scavenging	523:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	5	47	theme	H2	859:860	arg1	transition					845:854	the further transition	833:854	the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions	833:1129	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	3	48	with	hydrogel	509:516	arg1	functions					528:536	dual functions	523:536	dual functions of ROS scavenging	523:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	6	49	theme	cell	1212:1215	arg1	seeding					1217:1223	cell seeding	1212:1223	cell seeding	1212:1223	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	5	50	theme	MI	919:920	arg1	model					922:926	rat MI model	915:926	rat MI model	915:926	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	1	51	theme	excessive	124:132	arg1	species					150:156	The excessive reactive oxygen species	120:156	The excessive reactive oxygen species (ROS)	120:162	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	51	theme	excessive	124:132	arg1	ROS					159:161	ROS	159:161	ROS	159:161	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	5	52	theme	O.	901:902	arg1	Results					904:910	O2 and H2 O. Results	891:910	O2 and H2 O. Results of rat MI model	891:926	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	1	53	theme	reactive	134:141	arg1	species					150:156	The excessive reactive oxygen species	120:156	The excessive reactive oxygen species (ROS)	120:162	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	53	theme	reactive	134:141	arg1	ROS					159:161	ROS	159:161	ROS	159:161	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	54	theme	myocardial	230:239	arg1	infarction					241:250	myocardial infarction	230:250	myocardial infarction (MI)	230:255	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	54	theme	myocardial	230:239	arg1	MI					253:254	MI	253:254	MI	253:254	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	2	55	theme	nowadays	457:464	arg1	causes					441:446	the leading causes	429:446	the leading causes of death nowadays	429:464	In particular, the MI has become one of the leading causes of death nowadays.					
33169516	3	56	theme	scavenging	545:554	arg1	functions					528:536	dual functions	523:536	dual functions of ROS scavenging	523:554	In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O2 generating is fabricated for MI treatment in vivo.					
33169516	1	57	theme	oxygen	143:148	arg1	species					150:156	The excessive reactive oxygen species	120:156	The excessive reactive oxygen species (ROS)	120:162	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	1	57	theme	oxygen	143:148	arg1	ROS					159:161	ROS	159:161	ROS	159:161	The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs.					
33169516	0	58	theme	Injectable	55:64	arg1	Hydrogel					66:73	Injectable Hydrogel	55:73	Injectable Hydrogel	55:73	A Reactive Oxygen Species Scavenging and O2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo.					
33169516	6	59	theme	great	1321:1325	arg1	promise					1327:1333	a great promise	1319:1333	a great promise to be applied clinically	1319:1358	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	6	60	contain	has	1315:1317	arg1	scavenging					1277:1286	this ROS scavenging	1268:1286	this ROS scavenging	1268:1286	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	6	60	contain	has	1315:1317	arg2	promise					1327:1333	a great promise	1319:1333	a great promise to be applied clinically	1319:1358	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	6	60	contain	has	1315:1317	arg1	O2					1292:1293	O2	1292:1293	O2	1292:1293	With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O2 generating hydrogel has a great promise to be applied clinically.					
33169516	5	61	theme	further	837:843	arg1	transition					845:854	the further transition	833:854	the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions	833:1129	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	62	dep	remove	977:982	arg1	promote					1056:1062	promote	1056:1062	promote angiogenesis	1056:1075	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	62	dep	remove	977:982	arg1	reduce					1078:1083	reduce	1078:1083	reduce infarcted area	1078:1098	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	62	dep	remove	977:982	arg1	improve					1105:1111	improve	1105:1111	improve cardiac functions	1105:1129	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
33169516	5	62	dep	remove	977:982	arg1	inhibit					999:1005	inhibit	999:1005	inhibit cell apoptosis, increase M2/M1 macrophage ratio	999:1053	Addition of biocompatible and applicable catalase in vivo enables the further transition of H2 O2 , a major type of ROS, to O2 and H2 O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions.					
31223077	8	0	theme	in	1783:1784	arg1	system					1791:1796	the in situ system	1779:1796	the in situ system	1779:1796	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	6	1	theme	systemic	1386:1393	arg1	effects					1400:1406	systemic side effects	1386:1406	systemic side effects	1386:1406	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	2	2	from	attention	326:334	arg1	field					343:347	the field	339:347	the field of localized cancer chemotherapy	339:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	9	3	theme	anti-cancer	1837:1847	arg1	delivery					1854:1861	locoregional anti-cancer drug delivery	1824:1861	locoregional anti-cancer drug delivery	1824:1861	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials have also been discussed in detail in this article.					
31223077	4	4	theme	Poly	819:822	arg1	Acid					831:834	Poly Lactic Acid	819:834	Poly Lactic Acid (PLA)	819:840	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	4	theme	Poly	819:822	arg1	polymers					722:729	The polymers	718:729	The polymers such as chitosan	718:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	4	theme	Poly	819:822	arg1	chitosan					739:746	chitosan	739:746	chitosan	739:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	4	theme	Poly	819:822	arg1	PLA					837:839	PLA	837:839	PLA	837:839	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	1	5	theme	effective	181:189	arg1	delivery					191:198	the effective delivery	177:198	the effective delivery of anti-cancer agents	177:220	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	8	6	from	emphasis	1731:1738	arg1	types					1743:1747	types	1743:1747	types of polymers used to formulate the in situ system	1743:1796	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	7	7	theme	in	1553:1554	arg1	systems					1561:1567	approved polymer based in situ systems	1530:1567	approved polymer based in situ systems for prolonged locoregional activity	1530:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	2	8	from	release	463:469	arg1	field					343:347	the field	339:347	the field of localized cancer chemotherapy	339:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	6	9	theme	approach	1169:1176	arg1	use					1146:1148	The use	1142:1148	The use of in situ gelling approach	1142:1176	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	3	10	from	site	696:699	arg1	biodegradation					591:604	biodegradation	591:604	biodegradation	591:604	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	3	10	from	site	696:699	arg1	release					612:618	drug release	607:618	drug release	607:618	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	3	10	from	site	696:699	arg1	properties					640:649	physicochemical properties	624:649	physicochemical properties	624:649	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	6	11	theme	patient	1257:1263	arg1	survival					1221:1228	overall survival	1213:1228	overall survival	1213:1228	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	6	11	theme	patient	1257:1263	arg1	quality					1239:1245	life quality	1234:1245	life quality	1234:1245	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	1	12	theme	agents	215:220	arg1	delivery					191:198	the effective delivery	177:198	the effective delivery of anti-cancer agents	177:220	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	7	13	theme	polymer	1539:1545	arg1	systems					1561:1567	approved polymer based in situ systems	1530:1567	approved polymer based in situ systems for prolonged locoregional activity	1530:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	5	14	theme	hydrogel	1121:1128	arg1	depot					1135:1139	a hydrogel drug depot	1119:1139	a hydrogel drug depot	1119:1139	These formulations are administered in the solution form and on exposure to physiological environment such as temperature, pH or ionic composition they undergo phase conversion into a hydrogel drug depot.					
31223077	7	15	dep	States	1479:1484	arg1	States					1479:1484	United States	1472:1484	United States Food and Drug Administration (U.S. FDA)	1472:1524	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	15	dep	States	1479:1484	arg1	Food					1486:1489	Food	1486:1489	Food	1486:1489	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	15	dep	States	1479:1484	arg1	Administration					1500:1513	Drug Administration	1495:1513	Drug Administration	1495:1513	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	1	16	dep	concentration	255:267	arg1	to					247:248	to	247:248	to	247:248	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	8	17	theme	anti-cancer	1699:1709	arg1	agents					1711:1716	anti-cancer agents	1699:1716	anti-cancer agents	1699:1716	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	2	18	from	compliance	492:501	arg1	field					343:347	the field	339:347	the field of localized cancer chemotherapy	339:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	6	19	theme	life	1234:1237	arg1	quality					1239:1245	life quality	1234:1245	life quality	1234:1245	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	3	20	theme	physicochemical	624:638	arg1	properties					640:649	physicochemical properties	624:649	physicochemical properties	624:649	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	1	21	theme	drug	139:142	arg1	approach					164:171	a novel approach	156:171	a novel approach for the effective delivery of anti-cancer agents	156:220	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	1	21	theme	drug	139:142	arg1	delivery					144:151	Locoregional drug delivery	126:151	Locoregional drug delivery	126:151	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	4	22	theme	localized	908:916	arg1	vehicles					927:934	localized delivery vehicles	908:934	localized delivery vehicles	908:934	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	2	23	from	ease	414:417	arg1	field					343:347	the field	339:347	the field of localized cancer chemotherapy	339:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	2	24	dep	In	279:280	arg1	situ					282:285	situ	282:285	situ	282:285	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	1	25	theme	novel	158:162	arg1	approach					164:171	a novel approach	156:171	a novel approach for the effective delivery of anti-cancer agents	156:220	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	1	25	theme	novel	158:162	arg1	delivery					144:151	Locoregional drug delivery	126:151	Locoregional drug delivery	126:151	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	7	26	theme	Drug	1495:1498	arg1	States					1479:1484	United States	1472:1484	United States Food and Drug Administration (U.S. FDA)	1472:1524	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	26	theme	Drug	1495:1498	arg1	Administration					1500:1513	Drug Administration	1495:1513	Drug Administration	1495:1513	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	0	27	theme	Anti-cancer	91:101	arg1	Drugs					103:107	Anti-cancer Drugs	91:107	Anti-cancer Drugs	91:107	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	8	28	theme	polymers	1752:1759	arg1	types					1743:1747	types	1743:1747	types of polymers used to formulate the in situ system	1743:1796	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	8	29	dep	in	1658:1659	arg1	situ					1661:1664	situ	1661:1664	situ	1661:1664	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	4	30	dep	in	855:856	arg1	situ					858:861	situ	858:861	situ	858:861	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	6	31	theme	tumor	1321:1325	arg1	site					1313:1316	the site	1309:1316	the site of tumor	1309:1325	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	6	31	theme	tumor	1321:1325	arg1	tumor					1321:1325	tumor	1321:1325	tumor	1321:1325	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	2	32	theme	chemotherapy	369:380	arg1	field					343:347	the field	339:347	the field of localized cancer chemotherapy	339:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	0	33	theme	Harmonious	0:9	arg1	Biomaterials					11:22	Harmonious Biomaterials	0:22	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.	0:124	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	9	34	dep	In	1799:1800	arg1	situ					1802:1805	situ	1802:1805	situ	1802:1805	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials have also been discussed in detail in this article.					
31223077	7	35	theme	locoregional	1583:1594	arg1	activity					1596:1603	prolonged locoregional activity	1573:1603	prolonged locoregional activity	1573:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	4	36	theme	Hyaluronic	749:758	arg1	polymers					722:729	The polymers	718:729	The polymers such as chitosan	718:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	36	theme	Hyaluronic	749:758	arg1	HA					766:767	HA	766:767	HA	766:767	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	36	theme	Hyaluronic	749:758	arg1	chitosan					739:746	chitosan	739:746	chitosan	739:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	36	theme	Hyaluronic	749:758	arg1	Acid					760:763	Hyaluronic Acid	749:763	Hyaluronic Acid (HA)	749:768	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	5	37	theme	drug	1130:1133	arg1	depot					1135:1139	a hydrogel drug depot	1119:1139	a hydrogel drug depot	1119:1139	These formulations are administered in the solution form and on exposure to physiological environment such as temperature, pH or ionic composition they undergo phase conversion into a hydrogel drug depot.					
31223077	2	38	theme	drug	458:461	arg1	release					463:469	prolonged or sustained drug release	435:469	prolonged or sustained drug release	435:469	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	4	39	theme	Lactic	796:801	arg1	polymers					722:729	The polymers	718:729	The polymers such as chitosan	718:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	39	theme	Lactic	796:801	arg1	PGLA					809:812	PGLA	809:812	PGLA	809:812	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	39	theme	Lactic	796:801	arg1	Acid					803:806	Poly Glycolic Lactic Acid	782:806	Poly Glycolic Lactic Acid (PGLA)	782:813	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	39	theme	Lactic	796:801	arg1	chitosan					739:746	chitosan	739:746	chitosan	739:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	2	40	theme	targeted	395:402	arg1	delivery					404:411	their targeted delivery	389:411	their targeted delivery	389:411	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	7	41	theme	clinical	1453:1460	arg1	benefits					1462:1469	clinical benefits	1453:1469	clinical benefits	1453:1469	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	5	42	theme	solution	980:987	arg1	form					989:992	the solution form	976:992	the solution form	976:992	These formulations are administered in the solution form and on exposure to physiological environment such as temperature, pH or ionic composition they undergo phase conversion into a hydrogel drug depot.					
31223077	2	43	theme	paramount	316:324	arg1	attention					326:334	paramount attention	316:334	paramount attention in the field of localized cancer chemotherapy due to their targeted delivery	316:411	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	7	44	theme	favorable	1424:1432	arg1	profile					1441:1447	its favorable safety profile	1420:1447	its favorable safety profile	1420:1447	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	4	45	theme	Poly	782:785	arg1	polymers					722:729	The polymers	718:729	The polymers such as chitosan	718:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	45	theme	Poly	782:785	arg1	PGLA					809:812	PGLA	809:812	PGLA	809:812	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	45	theme	Poly	782:785	arg1	Acid					803:806	Poly Glycolic Lactic Acid	782:806	Poly Glycolic Lactic Acid (PGLA)	782:813	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	45	theme	Poly	782:785	arg1	chitosan					739:746	chitosan	739:746	chitosan	739:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	3	46	theme	Numerous	504:511	arg1	polymers					513:520	Numerous polymers	504:520	Numerous polymers	504:520	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	9	47	theme	In	1799:1800	arg1	formulations					1807:1818	In situ formulations	1799:1818	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials	1799:1909	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials have also been discussed in detail in this article.					
31223077	3	48	theme	implantation	704:715	arg1	implantation					704:715	implantation	704:715	implantation	704:715	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	3	48	theme	implantation	704:715	arg1	site					696:699	the site	692:699	the site of implantation	692:715	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	6	49	theme	side	1395:1398	arg1	effects					1400:1406	systemic side effects	1386:1406	systemic side effects	1386:1406	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	1	50	theme	tumors	240:245	arg1	concentration					255:267	the tumors to high concentration	236:267	the tumors to high concentration of drugs	236:276	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	7	51	dep	in	1553:1554	arg1	situ					1556:1559	situ	1556:1559	situ	1556:1559	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	4	52	theme	Lactic	824:829	arg1	Acid					831:834	Poly Lactic Acid	819:834	Poly Lactic Acid (PLA)	819:840	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	52	theme	Lactic	824:829	arg1	polymers					722:729	The polymers	718:729	The polymers such as chitosan	718:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	52	theme	Lactic	824:829	arg1	chitosan					739:746	chitosan	739:746	chitosan	739:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	52	theme	Lactic	824:829	arg1	PLA					837:839	PLA	837:839	PLA	837:839	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	6	53	theme	overall	1213:1219	arg1	survival					1221:1228	overall survival	1213:1228	overall survival	1213:1228	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	2	54	theme	In	279:280	arg1	systems					295:301	In situ gelling systems	279:301	In situ gelling systems	279:301	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	1	55	theme	high	250:253	arg1	concentration					255:267	the tumors to high concentration	236:267	the tumors to high concentration of drugs	236:276	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	9	56	theme	locoregional	1824:1835	arg1	delivery					1854:1861	locoregional anti-cancer drug delivery	1824:1861	locoregional anti-cancer drug delivery	1824:1861	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials have also been discussed in detail in this article.					
31223077	9	57	theme	drug	1849:1852	arg1	delivery					1854:1861	locoregional anti-cancer drug delivery	1824:1861	locoregional anti-cancer drug delivery	1824:1861	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials have also been discussed in detail in this article.					
31223077	2	58	theme	gelling	287:293	arg1	systems					295:301	In situ gelling systems	279:301	In situ gelling systems	279:301	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	8	59	theme	in	1658:1659	arg1	systems					1666:1672	in situ systems	1658:1672	in situ systems for targeted delivery of anti-cancer agents	1658:1716	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	7	60	theme	based	1547:1551	arg1	systems					1561:1567	approved polymer based in situ systems	1530:1567	approved polymer based in situ systems for prolonged locoregional activity	1530:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	4	61	theme	in	855:856	arg1	hydrogels					863:871	in situ hydrogels	855:871	in situ hydrogels	855:871	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	5	62	theme	physiological	1013:1025	arg1	environment					1027:1037	physiological environment	1013:1037	physiological environment	1013:1037	These formulations are administered in the solution form and on exposure to physiological environment such as temperature, pH or ionic composition they undergo phase conversion into a hydrogel drug depot.					
31223077	1	63	theme	anti-cancer	203:213	arg1	agents					215:220	anti-cancer agents	203:220	anti-cancer agents	203:220	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	7	64	theme	approved	1530:1537	arg1	systems					1561:1567	approved polymer based in situ systems	1530:1567	approved polymer based in situ systems for prolonged locoregional activity	1530:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	6	65	theme	gelling	1161:1167	arg1	approach					1169:1176	in situ gelling approach	1153:1176	in situ gelling approach	1153:1176	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	6	66	theme	cancer	1250:1255	arg1	patient					1257:1263	cancer patient	1250:1263	cancer patient	1250:1263	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	8	67	theme	targeted	1678:1685	arg1	delivery					1687:1694	targeted delivery	1678:1694	targeted delivery of anti-cancer agents	1678:1716	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	6	68	theme	in	1153:1154	arg1	approach					1169:1176	in situ gelling approach	1153:1176	in situ gelling approach	1153:1176	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	9	69	theme	clinical	1895:1902	arg1	trials					1904:1909	clinical trials	1895:1909	clinical trials	1895:1909	In situ formulations for locoregional anti-cancer drug delivery that are marketed and are under clinical trials have also been discussed in detail in this article.					
31223077	1	70	theme	Locoregional	126:137	arg1	approach					164:171	a novel approach	156:171	a novel approach for the effective delivery of anti-cancer agents	156:220	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	1	70	theme	Locoregional	126:137	arg1	delivery					144:151	Locoregional drug delivery	126:151	Locoregional drug delivery	126:151	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	7	71	theme	U.S.	1516:1519	arg1	States					1479:1484	United States	1472:1484	United States Food and Drug Administration (U.S. FDA)	1472:1524	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	71	theme	U.S.	1516:1519	arg1	FDA					1521:1523	U.S. FDA	1516:1523	U.S. FDA	1516:1523	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	0	72	theme	Locoregional	66:77	arg1	Delivery					79:86	Locoregional Delivery	66:86	Locoregional Delivery of Anti-cancer Drugs	66:107	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	3	73	theme	drug	607:610	arg1	release					612:618	drug release	607:618	drug release	607:618	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	8	74	theme	agents	1711:1716	arg1	delivery					1687:1694	targeted delivery	1678:1694	targeted delivery of anti-cancer agents	1678:1716	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	2	75	theme	improved	475:482	arg1	compliance					492:501	improved patient compliance	475:501	improved patient compliance	475:501	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	6	76	dep	in	1153:1154	arg1	situ					1156:1159	situ	1156:1159	situ	1156:1159	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	6	77	theme	drug	1301:1304	arg1	bioavailability					1282:1296	the bioavailability	1278:1296	the bioavailability of drug to the site of tumor	1278:1325	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	5	78	theme	ionic	1066:1070	arg1	composition					1072:1082	ionic composition	1066:1082	ionic composition	1066:1082	These formulations are administered in the solution form and on exposure to physiological environment such as temperature, pH or ionic composition they undergo phase conversion into a hydrogel drug depot.					
31223077	8	79	theme	special	1723:1729	arg1	emphasis					1731:1738	special emphasis	1723:1738	special emphasis on types of polymers used to formulate the in situ system	1723:1796	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	4	80	theme	delivery	918:925	arg1	vehicles					927:934	localized delivery vehicles	908:934	localized delivery vehicles	908:934	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	0	81	theme	Drugs	103:107	arg1	Delivery					79:86	Locoregional Delivery	66:86	Locoregional Delivery of Anti-cancer Drugs	66:107	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	7	82	contain	has	1526:1528	arg2	systems					1561:1567	approved polymer based in situ systems	1530:1567	approved polymer based in situ systems for prolonged locoregional activity	1530:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	82	contain	has	1526:1528	arg1	States					1479:1484	United States	1472:1484	United States Food and Drug Administration (U.S. FDA)	1472:1524	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	82	contain	has	1526:1528	arg1	Food					1486:1489	Food	1486:1489	Food	1486:1489	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	82	contain	has	1526:1528	arg1	Administration					1500:1513	Drug Administration	1495:1513	Drug Administration	1495:1513	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	82	contain	has	1526:1528	arg1	FDA					1521:1523	U.S. FDA	1516:1523	U.S. FDA	1516:1523	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	0	83	theme	Current	110:116	arg1	Trends					118:123	Current Trends	110:123	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.	0:124	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	8	84	dep	in	1783:1784	arg1	situ					1786:1789	situ	1786:1789	situ	1786:1789	This article discusses the rationale for developing in situ systems for targeted delivery of anti-cancer agents with special emphasis on types of polymers used to formulate the in situ system.					
31223077	2	85	theme	preparation	422:432	arg1	attention					326:334	paramount attention	316:334	paramount attention in the field of localized cancer chemotherapy due to their targeted delivery	316:411	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	2	85	theme	preparation	422:432	arg1	release					463:469	prolonged or sustained drug release	435:469	prolonged or sustained drug release	435:469	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	2	85	theme	preparation	422:432	arg1	ease					414:417	ease	414:417	ease of preparation	414:432	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	2	85	theme	preparation	422:432	arg1	compliance					492:501	improved patient compliance	475:501	improved patient compliance	475:501	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	2	86	theme	cancer	362:367	arg1	chemotherapy					369:380	localized cancer chemotherapy	352:380	localized cancer chemotherapy	352:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	6	87	theme	normal	1357:1362	arg1	cells					1364:1368	normal cells	1357:1368	normal cells	1357:1368	The use of in situ gelling approach has provided prospects to increase overall survival and life quality of cancer patient by enhancing the bioavailability of drug to the site of tumor by minimizing the exposure to normal cells and alleviating systemic side effects.					
31223077	1	88	theme	drugs	272:276	arg1	concentration					255:267	the tumors to high concentration	236:267	the tumors to high concentration of drugs	236:276	Locoregional drug delivery is a novel approach for the effective delivery of anti-cancer agents as it exposes the tumors to high concentration of drugs.					
31223077	3	89	theme	successful	655:664	arg1	targeting					666:674	successful targeting	655:674	successful targeting of the drugs	655:687	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	2	90	theme	localized	352:360	arg1	chemotherapy					369:380	localized cancer chemotherapy	352:380	localized cancer chemotherapy	352:380	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	0	91	theme	In	43:44	arg1	Approaches					51:60	In situ Approaches	43:60	In situ Approaches	43:60	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	7	92	theme	United	1472:1477	arg1	States					1479:1484	United States	1472:1484	United States Food and Drug Administration (U.S. FDA)	1472:1524	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	92	theme	United	1472:1477	arg1	Food					1486:1489	Food	1486:1489	Food	1486:1489	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	92	theme	United	1472:1477	arg1	Administration					1500:1513	Drug Administration	1495:1513	Drug Administration	1495:1513	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	92	theme	United	1472:1477	arg1	FDA					1521:1523	U.S. FDA	1516:1523	U.S. FDA	1516:1523	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	7	93	theme	prolonged	1573:1581	arg1	activity					1596:1603	prolonged locoregional activity	1573:1603	prolonged locoregional activity	1573:1603	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	2	94	theme	sustained	448:456	arg1	release					463:469	prolonged or sustained drug release	435:469	prolonged or sustained drug release	435:469	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	4	95	theme	Glycolic	787:794	arg1	polymers					722:729	The polymers	718:729	The polymers such as chitosan	718:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	95	theme	Glycolic	787:794	arg1	PGLA					809:812	PGLA	809:812	PGLA	809:812	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	95	theme	Glycolic	787:794	arg1	Acid					803:806	Poly Glycolic Lactic Acid	782:806	Poly Glycolic Lactic Acid (PGLA)	782:813	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	4	95	theme	Glycolic	787:794	arg1	chitosan					739:746	chitosan	739:746	chitosan	739:746	The polymers such as chitosan, Hyaluronic Acid (HA), poloxamer, Poly Glycolic Lactic Acid (PGLA) and Poly Lactic Acid (PLA) tend to form in situ hydrogels and have been exploited to develop localized delivery vehicles.					
31223077	0	96	theme	Approaches	51:60	arg1	Development					28:38	Development	28:38	Development of In situ Approaches	28:60	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	5	97	theme	phase	1097:1101	arg1	conversion					1103:1112	phase conversion	1097:1112	phase conversion into a hydrogel drug depot	1097:1139	These formulations are administered in the solution form and on exposure to physiological environment such as temperature, pH or ionic composition they undergo phase conversion into a hydrogel drug depot.					
31223077	0	98	dep	In	43:44	arg1	situ					46:49	situ	46:49	situ	46:49	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
31223077	2	99	theme	prolonged	435:443	arg1	release					463:469	prolonged or sustained drug release	435:469	prolonged or sustained drug release	435:469	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	3	100	theme	drugs	683:687	arg1	targeting					666:674	successful targeting	655:674	successful targeting of the drugs	655:687	Numerous polymers have been investigated for their properties like swelling along with biodegradation, drug release and physicochemical properties for successful targeting of the drugs at the site of implantation.					
31223077	2	101	theme	patient	484:490	arg1	compliance					492:501	improved patient compliance	475:501	improved patient compliance	475:501	In situ gelling systems have fetched paramount attention in the field of localized cancer chemotherapy due to their targeted delivery, ease of preparation, prolonged or sustained drug release and improved patient compliance.					
31223077	7	102	theme	safety	1434:1439	arg1	profile					1441:1447	its favorable safety profile	1420:1447	its favorable safety profile	1420:1447	Because of its favorable safety profile and clinical benefits, United States Food and Drug Administration (U.S. FDA) has approved polymer based in situ systems for prolonged locoregional activity.					
31223077	0	103	dep	Biomaterials	11:22	arg1	Trends					118:123	Current Trends	110:123	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.	0:124	Harmonious Biomaterials for Development of In situ Approaches for Locoregional Delivery of Anti-cancer Drugs: Current Trends.					
34098504	5	0	dep	achieve	967:973	arg1	ppm					1023:1025	150 and 300 ppm	1011:1025	ppm	1023:1025	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	10	1	theme	ppm	1418:1420	arg1	laminarin					1422:1430	300 ppm laminarin	1414:1430	300 ppm laminarin	1414:1430	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	2	2	theme	broiler	614:620	arg1	chickens					622:629	broiler chickens	614:629	broiler chickens	614:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	12	3	theme	toll-like	1934:1942	arg1	receptor					1944:1951	toll-like receptor 2	1934:1953	toll-like receptor 2 (TLR2)	1934:1960	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	3	theme	toll-like	1934:1942	arg1	TLR2					1956:1959	TLR2	1956:1959	TLR2	1956:1959	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	10	4	theme	final	1447:1451	arg1	±					1480:1480	2033 vs. 1906 ± 30.4	1466:1485	±	1480:1480	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	4	theme	final	1447:1451	arg1	weight					1458:1463	final body weight	1447:1463	final body weight (2033 vs. 1906 ± 30.4, P < 0.05)	1447:1496	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	5	dep	±	1641:1641	arg1	<					1651:1651	P < 0.05	1649:1656	P < 0.05	1649:1656	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	6	theme	1906	1475:1478	arg1	±					1480:1480	2033 vs. 1906 ± 30.4	1466:1485	±	1480:1480	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	6	theme	1906	1475:1478	arg1	weight					1458:1463	final body weight	1447:1463	final body weight (2033 vs. 1906 ± 30.4, P < 0.05)	1447:1496	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	13	7	theme	inclusion	2205:2213	arg1	level					2215:2219	the optimal inclusion level	2193:2219	the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens	2193:2317	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	9	8	theme	microbiota	1326:1335	arg1	analysis					1337:1344	microbiota analysis	1326:1344	microbiota analysis (high-throughput sequencing and QPCR)	1326:1382	Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR).					
34098504	7	9	from	performing	1157:1166	arg1	bird					1118:1121	one bird	1114:1121	one bird per pen from the control and best performing (300 ppm) laminarin groups	1114:1193	After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) laminarin groups were euthanized.					
34098504	10	10	theme	2033	1466:1469	arg1	±					1480:1480	2033 vs. 1906 ± 30.4	1466:1485	±	1480:1480	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	10	theme	2033	1466:1469	arg1	weight					1458:1463	final body weight	1447:1463	final body weight (2033 vs. 1906 ± 30.4, P < 0.05)	1447:1496	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	11	theme	control	1570:1576	arg1	group					1578:1582	the control group	1566:1582	the control group	1566:1582	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	2	12	from	markers	498:504	arg1	chickens					622:629	broiler chickens	614:629	broiler chickens	614:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	12	from	markers	498:504	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	12	13	theme	17A	1883:1885	arg1	receptor					1944:1951	toll-like receptor 2	1934:1953	toll-like receptor 2 (TLR2)	1934:1960	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	13	theme	17A	1883:1885	arg1	TLR2					1956:1959	TLR2	1956:1959	TLR2	1956:1959	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	13	theme	17A	1883:1885	arg1	expression					1857:1866	the expression	1853:1866	the expression of interleukin 17A (IL17A) in the duodenum	1853:1909	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	13	theme	17A	1883:1885	arg1	claudin					1912:1918	claudin 1	1912:1920	claudin 1 (CLDN1)	1912:1928	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	13	theme	17A	1883:1885	arg1	CLDN1					1923:1927	CLDN1	1923:1927	CLDN1	1923:1927	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	10	14	theme	P	1488:1488	arg1	<					1490:1490	P < 0.05	1488:1495	P < 0.05	1488:1495	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	5	15	theme	inclusion	993:1001	arg1	levels					1003:1008	the two laminarin inclusion levels	975:1008	the two laminarin inclusion levels	975:1008	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	13	16	theme	gastrointestinal	2271:2286	arg1	microbiota					2288:2297	the gastrointestinal microbiota	2267:2297	the gastrointestinal microbiota	2267:2297	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	1	17	theme	alternative	225:235	arg1	additives					242:250	alternative feed additives	225:250	alternative feed additives that will support growth through the promotion of gastrointestinal health and development	225:340	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	2	18	theme	microbiota	600:609	arg1	expression					461:470	the expression	457:470	the expression of nutrient transporters	457:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	18	theme	microbiota	600:609	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	18	theme	microbiota	600:609	arg1	composition					574:584	composition	574:584	composition of the caecal microbiota in broiler chickens	574:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	18	theme	microbiota	600:609	arg1	markers					498:504	markers	498:504	markers of inflammation and intestinal integrity in the small intestine	498:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	19	from	inflammation	509:520	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	10	20	theme	daily	1596:1600	arg1	intake					1607:1612	average daily feed intake	1588:1612	average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05)	1588:1657	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	20	theme	daily	1596:1600	arg1	±					1641:1641	114.1 vs. 106.0 and 104.5 ± 1.77	1615:1646	114.1 vs. 106.0 and 104.5 ± 1.77	1615:1646	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	3	21	theme	T3	803:804	arg1	pens/treatment					854:867	5 bird/pen; 16 pens/treatment	839:867	5 bird/pen; 16 pens/treatment	839:867	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	21	theme	T3	803:804	arg1	laminarin					828:836	(T3) basal diet + 300 ppm laminarin	802:836	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	0	22	from	health	112:117	arg1	broilers					122:129	broilers	122:129	broilers	122:129	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	3	23	theme	T2	766:767	arg1	laminarin					791:799	(T2) basal diet + 150 ppm laminarin	765:799	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	13	24	theme	growth	2158:2163	arg1	performance					2165:2175	growth performance	2158:2175	growth performance	2158:2175	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	3	25	theme	Ross	667:670	arg1	chicks					684:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks	632:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g)	632:709	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	25	theme	Ross	667:670	arg1	g					708:708	40.64 (3.43 SD) g	692:708	40.64 (3.43 SD) g	692:708	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	11	26	theme	Laminarin	1689:1697	arg1	supplementation					1699:1713	Laminarin supplementation	1689:1713	Laminarin supplementation at 300 ppm	1689:1724	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	3	27	theme	day-old	654:660	arg1	chicks					684:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks	632:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g)	632:709	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	27	theme	day-old	654:660	arg1	g					708:708	40.64 (3.43 SD) g	692:708	40.64 (3.43 SD) g	692:708	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	13	28	theme	microbiota	2288:2297	arg1	profile					2256:2262	a beneficial profile	2243:2262	a beneficial profile of the gastrointestinal microbiota	2243:2297	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	3	29	theme	ppm	824:826	arg1	pens/treatment					854:867	5 bird/pen; 16 pens/treatment	839:867	5 bird/pen; 16 pens/treatment	839:867	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	29	theme	ppm	824:826	arg1	laminarin					828:836	(T3) basal diet + 300 ppm laminarin	802:836	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	4	30	theme	laminarin-rich	910:923	arg1	spp					935:937	a laminarin-rich Laminaria spp	908:937	a laminarin-rich Laminaria spp	908:937	The basal diet was supplemented with a laminarin-rich Laminaria spp.					
34098504	2	31	from	performance	444:454	arg1	chickens					622:629	broiler chickens	614:629	broiler chickens	614:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	31	from	performance	444:454	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	3	32	theme	ppm	787:789	arg1	laminarin					791:799	(T2) basal diet + 150 ppm laminarin	765:799	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	0	33	theme	growth	72:77	arg1	performance					79:89	growth performance	72:89	growth performance	72:89	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	1	34	theme	broiler	168:174	arg1	production					184:193	broiler chicken production	168:193	broiler chicken production	168:193	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	3	35	theme	basal	807:811	arg1	pens/treatment					854:867	5 bird/pen; 16 pens/treatment	839:867	5 bird/pen; 16 pens/treatment	839:867	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	35	theme	basal	807:811	arg1	laminarin					828:836	(T3) basal diet + 300 ppm laminarin	802:836	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	4	36	theme	basal	875:879	arg1	diet					881:884	The basal diet	871:884	The basal diet	871:884	The basal diet was supplemented with a laminarin-rich Laminaria spp.					
34098504	0	37	from	Effects	0:6	arg1	performance					79:89	growth performance	72:89	growth performance	72:89	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	0	37	from	Effects	0:6	arg1	health					112:117	gastrointestinal health	95:117	gastrointestinal health	95:117	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	8	38	theme	Duodenal	1212:1219	arg1	tissues					1240:1246	Duodenal, jejunal and ileal tissues	1212:1246	Duodenal, jejunal and ileal tissues	1212:1246	Duodenal, jejunal and ileal tissues were collected for gene expression analysis.					
34098504	3	39	theme	basal	770:774	arg1	laminarin					791:799	(T2) basal diet + 150 ppm laminarin	765:799	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	13	40	theme	broiler	2302:2308	arg1	chickens					2310:2317	broiler chickens	2302:2317	broiler chickens	2302:2317	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	3	41	dep	diet	749:752	arg1	pens/treatment					854:867	5 bird/pen; 16 pens/treatment	839:867	5 bird/pen; 16 pens/treatment	839:867	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	41	dep	diet	749:752	arg1	laminarin					828:836	(T3) basal diet + 300 ppm laminarin	802:836	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	41	dep	diet	749:752	arg1	laminarin					791:799	(T2) basal diet + 150 ppm laminarin	765:799	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	42	theme	bird/pen	841:848	arg1	pens/treatment					854:867	5 bird/pen; 16 pens/treatment	839:867	5 bird/pen; 16 pens/treatment	839:867	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	42	theme	bird/pen	841:848	arg1	laminarin					828:836	(T3) basal diet + 300 ppm laminarin	802:836	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	2	43	theme	integrity	537:545	arg1	expression					461:470	the expression	457:470	the expression of nutrient transporters	457:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	43	theme	integrity	537:545	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	43	theme	integrity	537:545	arg1	composition					574:584	composition	574:584	composition of the caecal microbiota in broiler chickens	574:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	43	theme	integrity	537:545	arg1	markers					498:504	markers	498:504	markers of inflammation and intestinal integrity in the small intestine	498:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	8	44	theme	jejunal	1222:1228	arg1	tissues					1240:1246	Duodenal, jejunal and ileal tissues	1212:1246	Duodenal, jejunal and ileal tissues	1212:1246	Duodenal, jejunal and ileal tissues were collected for gene expression analysis.					
34098504	2	45	theme	inclusion	411:419	arg1	effects					392:398	the effects	388:398	the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens	388:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	1	46	from	Restriction	132:142	arg1	use					161:163	antimicrobial use	147:163	antimicrobial use	147:163	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	1	46	from	Restriction	132:142	arg1	production					184:193	broiler chicken production	168:193	broiler chicken production	168:193	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	13	47	theme	dietary	2130:2136	arg1	strategy					2138:2145	a promising dietary strategy	2118:2145	a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens	2118:2317	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	13	47	theme	dietary	2130:2136	arg1	supplementation					2084:2098	supplementation	2084:2098	supplementation with laminarin	2084:2113	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	8	48	theme	ileal	1234:1238	arg1	tissues					1240:1246	Duodenal, jejunal and ileal tissues	1212:1246	Duodenal, jejunal and ileal tissues	1212:1246	Duodenal, jejunal and ileal tissues were collected for gene expression analysis.					
34098504	2	49	theme	growth	437:442	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	0	50	theme	dietary	11:17	arg1	supplementation					19:33	dietary supplementation	11:33	dietary supplementation with a laminarin-rich extract	11:63	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	1	51	theme	gastrointestinal	302:317	arg1	health					319:324	gastrointestinal health	302:324	gastrointestinal health	302:324	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	2	52	theme	laminarin	424:432	arg1	inclusion					411:419	dietary inclusion	403:419	dietary inclusion of laminarin	403:432	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	4	53	theme	Laminaria	925:933	arg1	spp					935:937	a laminarin-rich Laminaria spp	908:937	a laminarin-rich Laminaria spp	908:937	The basal diet was supplemented with a laminarin-rich Laminaria spp.					
34098504	5	54	theme	65	949:950	arg1	%					951:951	%	951:951	%	951:951	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	2	55	theme	nutrient	475:482	arg1	transporters					484:495	nutrient transporters	475:495	nutrient transporters	475:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	10	56	theme	300	1414:1416	arg1	ppm					1418:1420	ppm	1418:1420	ppm	1418:1420	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	3	57	theme	broiler	676:682	arg1	chicks					684:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks	632:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g)	632:709	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	57	theme	broiler	676:682	arg1	g					708:708	40.64 (3.43 SD) g	692:708	40.64 (3.43 SD) g	692:708	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	8	58	theme	expression	1272:1281	arg1	analysis					1283:1290	gene expression analysis	1267:1290	gene expression analysis	1267:1290	Duodenal, jejunal and ileal tissues were collected for gene expression analysis.					
34098504	2	59	theme	study	365:369	arg1	objective					347:355	The objective	343:355	The objective of this study	343:369	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	0	60	theme	laminarin-rich	42:55	arg1	extract					57:63	a laminarin-rich extract	40:63	a laminarin-rich extract	40:63	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	11	61	theme	relative	1740:1747	arg1	abundance					1762:1770	the relative and absolute abundance	1736:1770	the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum	1736:1814	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	1	62	theme	feed	237:240	arg1	additives					242:250	alternative feed additives	225:250	alternative feed additives that will support growth through the promotion of gastrointestinal health and development	225:340	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	10	63	theme	P	1649:1649	arg1	<					1651:1651	P < 0.05	1649:1656	P < 0.05	1649:1656	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	9	64	dep	analysis	1337:1344	arg1	QPCR					1378:1381	QPCR	1378:1381	QPCR	1378:1381	Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR).					
34098504	9	64	dep	analysis	1337:1344	arg1	sequencing					1363:1372	high-throughput sequencing	1347:1372	high-throughput sequencing	1347:1372	Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR).					
34098504	10	65	with	supplementation	1393:1407	arg1	laminarin					1422:1430	300 ppm laminarin	1414:1430	300 ppm laminarin	1414:1430	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	11	66	from	abundance	1762:1770	arg1	caecum					1809:1814	the caecum	1805:1814	the caecum	1805:1814	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	10	67	theme	P	1544:1544	arg1	<					1546:1546	P < 0.05	1544:1551	P < 0.05	1544:1551	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	3	68	dep	40.64	692:696	arg1	SD					704:705	3.43 SD	699:705	3.43 SD	699:705	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	13	69	theme	optimal	2197:2203	arg1	level					2215:2219	the optimal inclusion level	2193:2219	the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens	2193:2317	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	11	70	theme	Bifidobacterium	1775:1789	arg1	abundance					1762:1770	the relative and absolute abundance	1736:1770	the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum	1736:1814	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	9	71	theme	Caecal	1293:1298	arg1	digesta					1300:1306	Caecal digesta	1293:1306	Caecal digesta	1293:1306	Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR).					
34098504	10	72	theme	body	1453:1456	arg1	±					1480:1480	2033 vs. 1906 ± 30.4	1466:1485	±	1480:1480	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	72	theme	body	1453:1456	arg1	weight					1458:1463	final body weight	1447:1463	final body weight (2033 vs. 1906 ± 30.4, P < 0.05)	1447:1496	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	1	73	theme	chicken	176:182	arg1	production					184:193	broiler chicken production	168:193	broiler chicken production	168:193	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	7	74	from	control	1140:1146	arg1	bird					1118:1121	one bird	1114:1121	one bird per pen from the control and best performing (300 ppm) laminarin groups	1114:1193	After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) laminarin groups were euthanized.					
34098504	10	75	dep	±	1480:1480	arg1	<					1490:1490	P < 0.05	1488:1495	P < 0.05	1488:1495	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	76	theme	average	1588:1594	arg1	intake					1607:1612	average daily feed intake	1588:1612	average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05)	1588:1657	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	76	theme	average	1588:1594	arg1	±					1641:1641	114.1 vs. 106.0 and 104.5 ± 1.77	1615:1646	114.1 vs. 106.0 and 104.5 ± 1.77	1615:1646	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	12	77	theme	SLC15A1/peptide	1998:2012	arg1	transporter					2014:2024	SLC15A1/peptide transporter 1	1998:2026	SLC15A1/peptide transporter 1 (SLC15A1/PepT1)	1998:2042	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	77	theme	SLC15A1/peptide	1998:2012	arg1	SLC15A1/PepT1					2029:2041	SLC15A1/PepT1	2029:2041	SLC15A1/PepT1	2029:2041	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	6	78	theme	feed	1047:1050	arg1	intake					1052:1057	feed intake	1047:1057	feed intake	1047:1057	Chick weights and feed intake was recorded weekly.					
34098504	3	79	theme	basal	743:747	arg1	control					755:761	control	755:761	control	755:761	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	79	theme	basal	743:747	arg1	diet					749:752	(T1) basal diet	738:752	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	11	80	theme	P	1792:1792	arg1	Bifidobacterium					1775:1789	Bifidobacterium	1775:1789	Bifidobacterium (P < 0.05)	1775:1800	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	11	80	theme	P	1792:1792	arg1	<					1794:1794	P < 0.05	1792:1799	P < 0.05	1792:1799	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	12	81	from	receptor	1944:1951	arg1	duodenum					1902:1909	the duodenum	1898:1909	the duodenum	1898:1909	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	13	82	with	supplementation	2084:2098	arg1	laminarin					2105:2113	laminarin	2105:2113	laminarin	2105:2113	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	0	83	from	performance	79:89	arg1	broilers					122:129	broilers	122:129	broilers	122:129	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	11	84	from	ppm	1722:1724	arg1	supplementation					1699:1713	Laminarin supplementation	1689:1713	Laminarin supplementation at 300 ppm	1689:1724	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	3	85	theme	T1	739:740	arg1	control					755:761	control	755:761	control	755:761	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	85	theme	T1	739:740	arg1	diet					749:752	(T1) basal diet	738:752	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	5	86	theme	laminarin	983:991	arg1	levels					1003:1008	the two laminarin inclusion levels	975:1008	the two laminarin inclusion levels	975:1008	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	9	87	theme	high-throughput	1347:1361	arg1	sequencing					1363:1372	high-throughput sequencing	1347:1372	high-throughput sequencing	1347:1372	Caecal digesta was collected for microbiota analysis (high-throughput sequencing and QPCR).					
34098504	2	88	theme	small	554:558	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	12	89	theme	interleukin	1871:1881	arg1	IL17A					1888:1892	IL17A	1888:1892	IL17A	1888:1892	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	89	theme	interleukin	1871:1881	arg1	17A					1883:1885	interleukin 17A	1871:1885	interleukin 17A (IL17A)	1871:1893	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	10	90	theme	feed	1602:1605	arg1	intake					1607:1612	average daily feed intake	1588:1612	average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05)	1588:1657	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	90	theme	feed	1602:1605	arg1	±					1641:1641	114.1 vs. 106.0 and 104.5 ± 1.77	1615:1646	114.1 vs. 106.0 and 104.5 ± 1.77	1615:1646	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	2	91	theme	caecal	593:598	arg1	microbiota					600:609	the caecal microbiota	589:609	the caecal microbiota	589:609	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	92	from	integrity	537:545	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	12	93	theme	Laminarin	1817:1825	arg1	supplementation					1827:1841	Laminarin supplementation	1817:1841	Laminarin supplementation	1817:1841	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	3	94	theme	male	662:665	arg1	chicks					684:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks	632:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g)	632:709	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	94	theme	male	662:665	arg1	g					708:708	40.64 (3.43 SD) g	692:708	40.64 (3.43 SD) g	692:708	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	95	theme	Two-hundred-and-forty	632:652	arg1	chicks					684:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks	632:689	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g)	632:709	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	95	theme	Two-hundred-and-forty	632:652	arg1	g					708:708	40.64 (3.43 SD) g	692:708	40.64 (3.43 SD) g	692:708	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	1	96	theme	antimicrobial	147:159	arg1	use					161:163	antimicrobial use	147:163	antimicrobial use	147:163	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	13	97	from	strategy	2138:2145	arg1	conclusion					2072:2081	conclusion	2072:2081	conclusion	2072:2081	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	2	98	theme	inflammation	509:520	arg1	expression					461:470	the expression	457:470	the expression of nutrient transporters	457:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	98	theme	inflammation	509:520	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	98	theme	inflammation	509:520	arg1	composition					574:584	composition	574:584	composition of the caecal microbiota in broiler chickens	574:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	98	theme	inflammation	509:520	arg1	markers					498:504	markers	498:504	markers of inflammation and intestinal integrity in the small intestine	498:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	7	99	theme	supplementation	1097:1111	arg1	days					1089:1092	35 days	1086:1092	35 days of supplementation	1086:1111	After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) laminarin groups were euthanized.					
34098504	10	100	theme	average	1502:1508	arg1	±					1536:1536	62.3 vs. 58.2 ± 0.95	1522:1541	62.3 vs. 58.2 ± 0.95	1522:1541	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	100	theme	average	1502:1508	arg1	gain					1516:1519	average daily gain	1502:1519	average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05)	1502:1552	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	2	101	from	expression	461:470	arg1	chickens					622:629	broiler chickens	614:629	broiler chickens	614:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	101	from	expression	461:470	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	12	102	theme	P	2058:2058	arg1	<					2060:2060	P < 0.05	2058:2065	P < 0.05	2058:2065	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	12	102	theme	P	2058:2058	arg1	ileum					2051:2055	the ileum	2047:2055	the ileum (P < 0.05)	2047:2066	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	0	103	theme	gastrointestinal	95:110	arg1	health					112:117	gastrointestinal health	95:117	gastrointestinal health	95:117	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	7	104	dep	control	1140:1146	arg1	groups					1188:1193	laminarin groups	1178:1193	laminarin groups	1178:1193	After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) laminarin groups were euthanized.					
34098504	3	105	theme	diet + 300	813:822	arg1	pens/treatment					854:867	5 bird/pen; 16 pens/treatment	839:867	5 bird/pen; 16 pens/treatment	839:867	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	105	theme	diet + 300	813:822	arg1	laminarin					828:836	(T3) basal diet + 300 ppm laminarin	802:836	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	3	106	theme	diet + 150	776:785	arg1	laminarin					791:799	(T2) basal diet + 150 ppm laminarin	765:799	(T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment)	738:868	Two-hundred-and-forty day-old male Ross 308 broiler chicks (40.64 (3.43 SD) g) were randomly assigned to: (T1) basal diet (control); (T2) basal diet + 150 ppm laminarin; (T3) basal diet + 300 ppm laminarin (5 bird/pen; 16 pens/treatment).					
34098504	5	107	theme	150	1011:1013	arg1	ppm					1023:1025	150 and 300 ppm	1011:1025	ppm	1023:1025	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	13	108	theme	promising	2120:2128	arg1	strategy					2138:2145	a promising dietary strategy	2118:2145	a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens	2118:2317	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	13	108	theme	promising	2120:2128	arg1	supplementation					2084:2098	supplementation	2084:2098	supplementation with laminarin	2084:2113	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	2	109	theme	intestinal	526:535	arg1	integrity					537:545	intestinal integrity	526:545	intestinal integrity	526:545	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	110	theme	dietary	403:409	arg1	inclusion					411:419	dietary inclusion	403:419	dietary inclusion of laminarin	403:432	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	5	111	theme	300	1019:1021	arg1	ppm					1023:1025	150 and 300 ppm	1011:1025	ppm	1023:1025	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	0	112	dep	performance	79:89	arg1	the					68:70	the	68:70	the	68:70	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	7	113	theme	laminarin	1178:1186	arg1	groups					1188:1193	laminarin groups	1178:1193	laminarin groups	1178:1193	After 35 days of supplementation, one bird per pen from the control and best performing (300 ppm) laminarin groups were euthanized.					
34098504	0	114	theme	supplementation	19:33	arg1	Effects					0:6	Effects	0:6	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers	0:129	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	12	115	from	expression	1857:1866	arg1	duodenum					1902:1909	the duodenum	1898:1909	the duodenum	1898:1909	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
34098504	10	116	dep	±	1536:1536	arg1	<					1546:1546	P < 0.05	1544:1551	P < 0.05	1544:1551	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	1	117	theme	health	319:324	arg1	promotion					289:297	the promotion	285:297	the promotion of gastrointestinal health and development	285:340	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	2	118	theme	transporters	484:495	arg1	expression					461:470	the expression	457:470	the expression of nutrient transporters	457:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	118	theme	transporters	484:495	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	118	theme	transporters	484:495	arg1	composition					574:584	composition	574:584	composition of the caecal microbiota in broiler chickens	574:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	118	theme	transporters	484:495	arg1	markers					498:504	markers	498:504	markers of inflammation and intestinal integrity in the small intestine	498:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	0	119	with	supplementation	19:33	arg1	extract					57:63	a laminarin-rich extract	40:63	a laminarin-rich extract	40:63	Effects of dietary supplementation with a laminarin-rich extract on the growth performance and gastrointestinal health in broilers.					
34098504	10	120	theme	daily	1510:1514	arg1	±					1536:1536	62.3 vs. 58.2 ± 0.95	1522:1541	62.3 vs. 58.2 ± 0.95	1522:1541	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	10	120	theme	daily	1510:1514	arg1	gain					1516:1519	average daily gain	1502:1519	average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05)	1502:1552	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	13	121	theme	beneficial	2245:2254	arg1	profile					2256:2262	a beneficial profile	2243:2262	a beneficial profile of the gastrointestinal microbiota	2243:2297	In conclusion, supplementation with laminarin is a promising dietary strategy to enhance growth performance and 300 ppm was the optimal inclusion level with which to promote a beneficial profile of the gastrointestinal microbiota in broiler chickens.					
34098504	1	122	theme	development	330:340	arg1	promotion					289:297	the promotion	285:297	the promotion of gastrointestinal health and development	285:340	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	5	123	theme	%	951:951	arg1	extract					940:946	extract	940:946	extract (65% laminarin)	940:962	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	5	123	theme	%	951:951	arg1	laminarin					953:961	65% laminarin	949:961	65% laminarin	949:961	extract (65% laminarin) to achieve the two laminarin inclusion levels (150 and 300 ppm).					
34098504	2	124	from	effects	392:398	arg1	composition					574:584	composition	574:584	composition of the caecal microbiota in broiler chickens	574:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	124	from	effects	392:398	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	124	from	effects	392:398	arg1	expression					461:470	the expression	457:470	the expression of nutrient transporters	457:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	124	from	effects	392:398	arg1	markers					498:504	markers	498:504	markers of inflammation and intestinal integrity in the small intestine	498:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	6	125	theme	Chick	1029:1033	arg1	weights					1035:1041	Chick weights	1029:1041	Chick weights	1029:1041	Chick weights and feed intake was recorded weekly.					
34098504	8	126	theme	gene	1267:1270	arg1	analysis					1283:1290	gene expression analysis	1267:1290	gene expression analysis	1267:1290	Duodenal, jejunal and ileal tissues were collected for gene expression analysis.					
34098504	2	127	from	intestine	560:568	arg1	expression					461:470	the expression	457:470	the expression of nutrient transporters	457:495	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	127	from	intestine	560:568	arg1	performance					444:454	growth performance	437:454	growth performance	437:454	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	127	from	intestine	560:568	arg1	composition					574:584	composition	574:584	composition of the caecal microbiota in broiler chickens	574:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	127	from	intestine	560:568	arg1	markers					498:504	markers	498:504	markers of inflammation and intestinal integrity in the small intestine	498:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	10	128	theme	Dietary	1385:1391	arg1	supplementation					1393:1407	Dietary supplementation	1385:1407	Dietary supplementation with 300 ppm laminarin	1385:1430	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	11	129	theme	absolute	1753:1760	arg1	abundance					1762:1770	the relative and absolute abundance	1736:1770	the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum	1736:1814	Laminarin supplementation at 300 ppm increased the relative and absolute abundance of Bifidobacterium (P < 0.05) in the caecum.					
34098504	1	130	theme	additives	242:250	arg1	exploration					210:220	the exploration	206:220	the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development	206:340	Restriction in antimicrobial use in broiler chicken production is driving the exploration of alternative feed additives that will support growth through the promotion of gastrointestinal health and development.					
34098504	2	131	from	composition	574:584	arg1	chickens					622:629	broiler chickens	614:629	broiler chickens	614:629	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	2	131	from	composition	574:584	arg1	intestine					560:568	the small intestine	550:568	the small intestine	550:568	The objective of this study was to determine the effects of dietary inclusion of laminarin on growth performance, the expression of nutrient transporters, markers of inflammation and intestinal integrity in the small intestine and composition of the caecal microbiota in broiler chickens.					
34098504	10	132	theme	other	1675:1679	arg1	groups					1681:1686	all other groups	1671:1686	all other groups	1671:1686	Dietary supplementation with 300 ppm laminarin increased both final body weight (2033 vs. 1906 ± 30.4, P < 0.05) and average daily gain (62.3 vs. 58.2 ± 0.95, P < 0.05) compared to the control group and average daily feed intake (114.1 vs. 106.0 and 104.5 ± 1.77, P < 0.05) compared to all other groups.					
34098504	12	133	from	claudin	1912:1918	arg1	duodenum					1902:1909	the duodenum	1898:1909	the duodenum	1898:1909	Laminarin supplementation increased the expression of interleukin 17A (IL17A) in the duodenum, claudin 1 (CLDN1) and toll-like receptor 2 (TLR2) in the jejunum and IL17A, CLDN1 and SLC15A1/peptide transporter 1 (SLC15A1/PepT1) in the ileum (P < 0.05).					
33788085	0	0	theme	Tannic	86:91	arg1	Acid					93:96	Tannic Acid	86:96	Tannic Acid	86:96	Increasing the Production of β-Glucan from Saccharomyces carlsbergensis RU01 by Using Tannic Acid.					
33788085	3	1	theme	w/v	621:623	arg1	acid					632:635	0.1% w/v tannic acid	616:635	0.1% w/v tannic acid	616:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	7	2	dep	Fourier	1047:1053	arg1	transform					1055:1063	transform	1055:1063	transform infrared (FTIR) spectroscopy	1055:1092	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	1	3	from	yeast	161:165	arg1	production					136:145	β-glucan production	127:145	β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01,	127:201	In this study, we increased β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01, by using tannic acid.					
33788085	2	4	theme	yeast	343:347	arg1	medium					359:364	yeast malt (YM) medium	343:364	yeast malt (YM) medium supplemented with 0.1% w/v tannic acid	343:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	7	5	theme	%	1189:1189	arg1	acid					1202:1205	0.1% w/v tannic acid	1186:1205	0.1% w/v tannic acid	1186:1205	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	4	6	theme	%	694:694	arg1	acid					707:710	0.1% w/v tannic acid	691:710	0.1% w/v tannic acid	691:710	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	5	7	theme	β-glucan	856:863	arg1	content					865:871	the β-glucan content	852:871	the β-glucan content	852:871	Tannic acid enhanced the β-glucan content by up to 42.23%.					
33788085	7	8	theme	β-glucan	1023:1030	arg1	composition					1032:1042	the β-glucan composition	1019:1042	the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy	1019:1092	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	3	9	theme	0.1	616:618	arg1	%					619:619	%	619:619	%	619:619	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	7	10	theme	w/v	1191:1193	arg1	acid					1202:1205	0.1% w/v tannic acid	1186:1205	0.1% w/v tannic acid	1186:1205	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	6	11	theme	shake	981:985	arg1	culture					998:1004	the shake flask (SF) culture	977:1004	the shake flask (SF) culture	977:1004	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	7	12	contain	had	1207:1209	arg2	proportion					1220:1229	a higher proportion	1211:1229	a higher proportion of polysaccharide	1211:1247	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	7	12	contain	had	1207:1209	arg1	β-glucan					1110:1117	the β-glucan	1106:1117	the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid	1106:1205	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	7	13	theme	composition	1032:1042	arg1	Analysis					1007:1014	Analysis	1007:1014	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy	1007:1092	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	0	14	from	carlsbergensis	57:70	arg1	Production					15:24	the Production	11:24	the Production of β-Glucan from Saccharomyces carlsbergensis RU01	11:75	Increasing the Production of β-Glucan from Saccharomyces carlsbergensis RU01 by Using Tannic Acid.					
33788085	4	15	theme	w/v	696:698	arg1	acid					707:710	0.1% w/v tannic acid	691:710	0.1% w/v tannic acid	691:710	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	8	16	used	used	1325:1328	arg2	β-glucans					1288:1296	β-glucans	1288:1296	β-glucans from brewer's yeast	1288:1316	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	8	16	used	used	1325:1328	arg2	foods					1358:1362	prebiotic and functional foods	1333:1362	prebiotic and functional foods for human health	1333:1379	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	4	17	theme	0.1	691:693	arg1	%					694:694	%	694:694	%	694:694	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	7	18	theme	MDS	1157:1159	arg1	medium					1161:1166	MDS medium	1157:1166	MDS medium supplemented with 0.1% w/v tannic acid	1157:1205	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	4	19	dep	%	804:804	arg1	w/v					806:808	w/v	806:808	0.28±0.01% w/v	795:808	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	3	20	theme	tannic	625:630	arg1	acid					632:635	0.1% w/v tannic acid	616:635	0.1% w/v tannic acid	616:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	4	21	theme	cell	744:747	arg1	DCW					757:759	DCW	757:759	DCW	757:759	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	4	21	theme	cell	744:747	arg1	weight					749:754	the dry cell weight	736:754	the dry cell weight (DCW)	736:760	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	2	22	theme	electron	265:272	arg1	HPF-TEM					286:292	HPF-TEM	286:292	HPF-TEM	286:292	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	22	theme	electron	265:272	arg1	microscopy					274:283	transmission electron microscopy	252:283	transmission electron microscopy (HPF-TEM)	252:293	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	6	23	from	production	899:908	arg1	reactor					930:936	the stirred tank reactor	913:936	the stirred tank reactor (STR)	913:942	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	6	23	from	production	899:908	arg1	STR					939:941	STR	939:941	STR	939:941	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	6	24	theme	SF	994:995	arg1	culture					998:1004	the shake flask (SF) culture	977:1004	the shake flask (SF) culture	977:1004	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	5	25	dep	42.23	882:886	arg1	to					879:880	to	879:880	to	879:880	Tannic acid enhanced the β-glucan content by up to 42.23%.					
33788085	8	26	from	yeast	1312:1316	arg1	foods					1358:1362	prebiotic and functional foods	1333:1362	prebiotic and functional foods for human health	1333:1379	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	8	26	from	yeast	1312:1316	arg1	β-glucans					1288:1296	β-glucans	1288:1296	β-glucans from brewer's yeast	1288:1316	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	8	27	theme	functional	1347:1356	arg1	foods					1358:1362	prebiotic and functional foods	1333:1362	prebiotic and functional foods for human health	1333:1379	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	8	27	theme	functional	1347:1356	arg1	β-glucans					1288:1296	β-glucans	1288:1296	β-glucans from brewer's yeast	1288:1316	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	7	28	theme	0.1	1186:1188	arg1	%					1189:1189	%	1189:1189	%	1189:1189	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	7	29	dep	infrared	1065:1072	arg1	FTIR					1075:1078	FTIR	1075:1078	FTIR	1075:1078	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	2	30	theme	YM	355:356	arg1	medium					359:364	yeast malt (YM) medium	343:364	yeast malt (YM) medium supplemented with 0.1% w/v tannic acid	343:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	7	31	theme	tannic	1195:1200	arg1	acid					1202:1205	0.1% w/v tannic acid	1186:1205	0.1% w/v tannic acid	1186:1205	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	4	32	theme	MDS	662:664	arg1	medium					666:671	MDS medium	662:671	MDS medium supplemented with 0.1% w/v tannic acid	662:710	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	2	33	theme	w/v	389:391	arg1	acid					400:403	0.1% w/v tannic acid	384:403	0.1% w/v tannic acid	384:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	34	theme	tannic	393:398	arg1	acid					400:403	0.1% w/v tannic acid	384:403	0.1% w/v tannic acid	384:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	7	35	theme	polysaccharide	1234:1247	arg1	proportion					1220:1229	a higher proportion	1211:1229	a higher proportion of polysaccharide	1211:1247	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	3	36	theme	sulfate	577:583	arg1	medium					591:596	0.1% w/v diammonium sulfate (MDS) medium	557:596	0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid	557:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	5	37	theme	Tannic	831:836	arg1	acid					838:841	Tannic acid	831:841	Tannic acid	831:841	Tannic acid enhanced the β-glucan content by up to 42.23%.					
33788085	2	38	theme	%	387:387	arg1	acid					400:403	0.1% w/v tannic acid	384:403	0.1% w/v tannic acid	384:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	3	39	theme	%	538:538	arg1	molasses					544:551	3% w/v molasses	537:551	3% w/v molasses	537:551	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	1	40	theme	Saccharomyces	168:180	arg1	yeast					161:165	brewer's yeast	152:165	brewer's yeast	152:165	In this study, we increased β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01, by using tannic acid.					
33788085	1	40	theme	Saccharomyces	168:180	arg1	carlsbergensis					182:195	Saccharomyces carlsbergensis RU01	168:200	Saccharomyces carlsbergensis RU01	168:200	In this study, we increased β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01, by using tannic acid.					
33788085	2	41	theme	0.1	384:386	arg1	%					387:387	%	387:387	%	387:387	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	0	42	theme	β-Glucan	29:36	arg1	Production					15:24	the Production	11:24	the Production of β-Glucan from Saccharomyces carlsbergensis RU01	11:75	Increasing the Production of β-Glucan from Saccharomyces carlsbergensis RU01 by Using Tannic Acid.					
33788085	4	43	dep	%	824:824	arg1	w/w					826:828	w/w	826:828	11.99±0.04% w/w	814:828	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	3	44	theme	w/v	562:564	arg1	sulfate					577:583	0.1% w/v diammonium sulfate	557:583	0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid	557:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	3	44	theme	w/v	562:564	arg1	MDS					586:588	MDS	586:588	MDS	586:588	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	3	45	theme	w/v	540:542	arg1	molasses					544:551	3% w/v molasses	537:551	3% w/v molasses	537:551	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	6	46	theme	tank	925:928	arg1	reactor					930:936	the stirred tank reactor	913:936	the stirred tank reactor (STR)	913:942	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	6	46	theme	tank	925:928	arg1	STR					939:941	STR	939:941	STR	939:941	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	8	47	theme	human	1368:1372	arg1	health					1374:1379	human health	1368:1379	human health	1368:1379	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	3	48	theme	diammonium	566:575	arg1	sulfate					577:583	0.1% w/v diammonium sulfate	557:583	0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid	557:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	3	48	theme	diammonium	566:575	arg1	MDS					586:588	MDS	586:588	MDS	586:588	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	4	49	theme	dry	740:742	arg1	DCW					757:759	DCW	757:759	DCW	757:759	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	4	49	theme	dry	740:742	arg1	weight					749:754	the dry cell weight	736:754	the dry cell weight (DCW)	736:760	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	8	50	theme	prebiotic	1333:1341	arg1	foods					1358:1362	prebiotic and functional foods	1333:1362	prebiotic and functional foods for human health	1333:1379	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	8	50	theme	prebiotic	1333:1341	arg1	β-glucans					1288:1296	β-glucans	1288:1296	β-glucans from brewer's yeast	1288:1316	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	8	51	theme	animal	1388:1393	arg1	feed					1395:1398	animal feed	1388:1398	animal feed	1388:1398	In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.					
33788085	0	52	theme	Saccharomyces	43:55	arg1	carlsbergensis					57:70	Saccharomyces carlsbergensis RU01	43:75	Saccharomyces carlsbergensis RU01	43:75	Increasing the Production of β-Glucan from Saccharomyces carlsbergensis RU01 by Using Tannic Acid.					
33788085	4	53	theme	β-glucan	771:778	arg1	production					780:789	the β-glucan production	767:789	the β-glucan production	767:789	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	4	53	theme	β-glucan	771:778	arg1	%					804:804	0.28±0.01%	795:804	0.28±0.01% w/v	795:808	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	3	54	theme	3	537:537	arg1	%					538:538	%	538:538	%	538:538	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	3	55	from	carlsbergensis	500:513	arg1	production					469:478	The production	465:478	The production of β-glucan from S. carlsbergensis RU01	465:518	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	4	56	theme	tannic	700:705	arg1	acid					707:710	0.1% w/v tannic acid	691:710	0.1% w/v tannic acid	691:710	The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w.					
33788085	3	57	theme	0.1	557:559	arg1	%					560:560	%	560:560	%	560:560	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	2	58	theme	transmission	252:263	arg1	HPF-TEM					286:292	HPF-TEM	286:292	HPF-TEM	286:292	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	58	theme	transmission	252:263	arg1	microscopy					274:283	transmission electron microscopy	252:283	transmission electron microscopy (HPF-TEM)	252:293	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	59	theme	cell	319:322	arg1	wall					324:327	the yeast cell wall	309:327	the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid	309:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	59	theme	cell	319:322	arg1	thicker					409:415	thicker	409:415	thicker	409:415	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	6	60	theme	flask	987:991	arg1	culture					998:1004	the shake flask (SF) culture	977:1004	the shake flask (SF) culture	977:1004	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	6	61	theme	β-Glucan	890:897	arg1	production					899:908	β-Glucan production	890:908	β-Glucan production in the stirred tank reactor (STR)	890:942	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	3	62	theme	β-glucan	483:490	arg1	production					469:478	The production	465:478	The production of β-glucan from S. carlsbergensis RU01	465:518	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	3	63	theme	%	560:560	arg1	sulfate					577:583	0.1% w/v diammonium sulfate	557:583	0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid	557:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	3	63	theme	%	560:560	arg1	MDS					586:588	MDS	586:588	MDS	586:588	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	2	64	theme	yeast	313:317	arg1	wall					324:327	the yeast cell wall	309:327	the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid	309:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	64	theme	yeast	313:317	arg1	thicker					409:415	thicker	409:415	thicker	409:415	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	1	65	theme	tannic	212:217	arg1	acid					219:222	tannic acid	212:222	tannic acid	212:222	In this study, we increased β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01, by using tannic acid.					
33788085	7	66	theme	infrared	1065:1072	arg1	spectroscopy					1081:1092	infrared (FTIR) spectroscopy	1065:1092	infrared (FTIR) spectroscopy	1065:1092	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	6	67	theme	stirred	917:923	arg1	reactor					930:936	the stirred tank reactor	913:936	the stirred tank reactor (STR)	913:942	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	6	67	theme	stirred	917:923	arg1	STR					939:941	STR	939:941	STR	939:941	β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture.					
33788085	1	68	theme	β-glucan	127:134	arg1	production					136:145	β-glucan production	127:145	β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01,	127:201	In this study, we increased β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01, by using tannic acid.					
33788085	7	69	theme	carlsbergensis	1125:1138	arg1	β-glucan					1110:1117	the β-glucan	1106:1117	the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid	1106:1205	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33788085	2	70	theme	YM	448:449	arg1	medium					451:456	YM medium	448:456	YM medium alone	448:462	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	3	71	theme	%	619:619	arg1	acid					632:635	0.1% w/v tannic acid	616:635	0.1% w/v tannic acid	616:635	The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid.					
33788085	2	72	theme	High-pressure	225:237	arg1	freezing					239:246	High-pressure freezing	225:246	High-pressure freezing	225:246	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	2	73	theme	malt	349:352	arg1	medium					359:364	yeast malt (YM) medium	343:364	yeast malt (YM) medium supplemented with 0.1% w/v tannic acid	343:403	High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone.					
33788085	7	74	theme	higher	1213:1218	arg1	proportion					1220:1229	a higher proportion	1211:1229	a higher proportion of polysaccharide	1211:1247	Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control.					
33721803	6	0	theme	novel	1110:1114	arg1	agents					1147:1152	novel highly efficient antibacterial agents	1110:1152	novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed	1110:1254	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	6	0	theme	novel	1110:1114	arg1	applicability					1093:1105	their applicability	1087:1105	their applicability	1087:1105	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	3	1	dep	K.	607:608	arg1	pneumoniae					610:619	K. pneumoniae	607:619	K. pneumoniae	607:619	Their composition, structure and morphology were studied, followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains.					
33721803	2	2	theme	Gr	366:367	arg1	NCs					379:381	graphene(Gr)/CS/Fe3O4 NCs	357:381	graphene(Gr)/CS/Fe3O4 NCs	357:381	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	6	3	theme	wide	1175:1178	arg1	range					1180:1184	a wide range	1173:1184	a wide range	1173:1184	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	6	4	theme	applications	1200:1211	arg1	range					1180:1184	a wide range	1173:1184	a wide range	1173:1184	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	4	5	dep	NCs	746:748	arg1	%					814:814	91 and 77 %	804:814	105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae	751:843	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	4	5	dep	NCs	746:748	arg1	%					762:762	105 and 69 %	751:762	105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae	751:843	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	5	6	theme	minimum	865:871	arg1	MIC					899:901	MIC	899:901	MIC	899:901	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	5	6	theme	minimum	865:871	arg1	concentration					884:896	The minimum inhibitory concentration	861:896	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae	861:961	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	5	6	theme	minimum	865:871	arg1	μg/mL					978:982	60 and 70 μg/mL	968:982	60 and 70 μg/mL	968:982	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	6	7	theme	biomedical	1189:1198	arg1	applications					1200:1211	biomedical applications	1189:1211	biomedical applications	1189:1211	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	2	8	theme	simple	403:408	arg1	method					423:428	simple hydrothermal method	403:428	simple hydrothermal method	403:428	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	5	9	theme	Gr/CS/Fe3O4	907:917	arg1	NCs					919:921	Gr/CS/Fe3O4 NCs	907:921	Gr/CS/Fe3O4 NCs	907:921	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	2	10	theme	chitosan	312:319	arg1	nanocomposites					332:345	chitosan (CS)/Fe3O4 nanocomposites	312:345	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs)	263:351	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	1	11	theme	new	237:239	arg1	nanocomposites					201:214	chitosan-containing nanocomposites	181:214	chitosan-containing nanocomposites	181:214	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	1	11	theme	new	237:239	arg1	agents					255:260	new antibacterial agents	237:260	new antibacterial agents	237:260	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	0	12	theme	efficient	7:15	arg1	activity					31:38	Highly efficient antibacterial activity	0:38	Highly efficient antibacterial activity of graphene/chitosan/magnetite	0:69	Highly efficient antibacterial activity of graphene/chitosan/magnetite nanocomposites against ESBL-producing Pseudomonas aeruginosa and Klebsiella pneumoniae.					
33721803	6	13	dep	activity	1029:1036	arg1	The					999:1001	The	999:1001	The	999:1001	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	1	14	theme	antibacterial	241:253	arg1	nanocomposites					201:214	chitosan-containing nanocomposites	181:214	chitosan-containing nanocomposites	181:214	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	1	14	theme	antibacterial	241:253	arg1	agents					255:260	new antibacterial agents	237:260	new antibacterial agents	237:260	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	6	15	theme	antibacterial	1220:1232	arg1	properties					1234:1243	antibacterial properties	1220:1243	antibacterial properties	1220:1243	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	4	16	theme	higher	764:769	arg1	%					762:762	105 and 69 %	751:762	105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae	751:843	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	4	17	theme	Fe3O4	723:727	arg1	NPs					729:731	Fe3O4 NPs	723:731	Fe3O4 NPs	723:731	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	4	18	theme	higher	816:821	arg1	%					814:814	91 and 77 %	804:814	105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae	751:843	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	5	19	dep	K.	949:950	arg1	pneumoniae					952:961	K. pneumoniae	949:961	K. pneumoniae	949:961	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	6	20	dep	novel	1110:1114	arg1	efficient					1123:1131	efficient	1123:1131	efficient	1123:1131	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	4	21	theme	higher	681:686	arg1	activity					702:709	significantly higher antibacterial activity	667:709	significantly higher antibacterial activity	667:709	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	0	22	theme	antibacterial	17:29	arg1	activity					31:38	Highly efficient antibacterial activity	0:38	Highly efficient antibacterial activity of graphene/chitosan/magnetite	0:69	Highly efficient antibacterial activity of graphene/chitosan/magnetite nanocomposites against ESBL-producing Pseudomonas aeruginosa and Klebsiella pneumoniae.					
33721803	2	23	theme	/Fe3O4	325:330	arg1	nanocomposites					332:345	chitosan (CS)/Fe3O4 nanocomposites	312:345	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs)	263:351	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	1	24	theme	chitosan-containing	181:199	arg1	nanocomposites					201:214	chitosan-containing nanocomposites	181:214	chitosan-containing nanocomposites	181:214	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	1	24	theme	chitosan-containing	181:199	arg1	agents					255:260	new antibacterial agents	237:260	new antibacterial agents	237:260	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	0	25	theme	graphene/chitosan/magnetite	43:69	arg1	activity					31:38	Highly efficient antibacterial activity	0:38	Highly efficient antibacterial activity of graphene/chitosan/magnetite	0:69	Highly efficient antibacterial activity of graphene/chitosan/magnetite nanocomposites against ESBL-producing Pseudomonas aeruginosa and Klebsiella pneumoniae.					
33721803	2	26	theme	graphene	357:364	arg1	NCs					379:381	graphene(Gr)/CS/Fe3O4 NCs	357:381	graphene(Gr)/CS/Fe3O4 NCs	357:381	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	4	27	theme	K.	831:832	arg1	pneumoniae					834:843	K. pneumoniae	831:843	K. pneumoniae	831:843	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	6	28	theme	NCs	1074:1076	arg1	activity					1029:1036	synergistic antibacterial activity	1003:1036	synergistic antibacterial activity	1003:1036	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	6	28	theme	NCs	1074:1076	arg1	synthesis					1049:1057	facile synthesis	1042:1057	facile synthesis	1042:1057	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	4	29	theme	CS/Fe3O4	737:744	arg1	NCs					746:748	CS/Fe3O4 NCs	737:748	CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively)	737:858	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	2	30	theme	hydrothermal	410:421	arg1	method					423:428	simple hydrothermal method	403:428	simple hydrothermal method	403:428	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	6	31	theme	facile	1042:1047	arg1	synthesis					1049:1057	facile synthesis	1042:1057	facile synthesis	1042:1057	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	5	32	theme	NCs	919:921	arg1	MIC					899:901	MIC	899:901	MIC	899:901	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	5	32	theme	NCs	919:921	arg1	concentration					884:896	The minimum inhibitory concentration	861:896	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae	861:961	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	5	32	theme	NCs	919:921	arg1	μg/mL					978:982	60 and 70 μg/mL	968:982	60 and 70 μg/mL	968:982	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	2	33	theme	/CS/Fe3O4	369:377	arg1	NCs					379:381	graphene(Gr)/CS/Fe3O4 NCs	357:381	graphene(Gr)/CS/Fe3O4 NCs	357:381	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	3	34	theme	bacterial	621:629	arg1	strains					631:637	bacterial strains	621:637	bacterial strains	621:637	Their composition, structure and morphology were studied, followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains.					
33721803	1	35	theme	present	166:172	arg1	study					174:178	the present study	162:178	the present study	162:178	In the present study, chitosan-containing nanocomposites were investigated as new antibacterial agents.					
33721803	6	36	theme	antibacterial	1133:1145	arg1	agents					1147:1152	novel highly efficient antibacterial agents	1110:1152	novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed	1110:1254	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	6	36	theme	antibacterial	1133:1145	arg1	applicability					1093:1105	their applicability	1087:1105	their applicability	1087:1105	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	4	37	theme	Gr/CS/Fe3O4	644:654	arg1	NCs					656:658	The Gr/CS/Fe3O4 NCs	640:658	The Gr/CS/Fe3O4 NCs	640:658	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	2	38	theme	Magnetite	263:271	arg1	NPs					296:298	NPs	296:298	NPs	296:298	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	2	38	theme	Magnetite	263:271	arg1	nanoparticles					281:293	Magnetite (Fe3O4) nanoparticles	263:293	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs)	263:351	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	2	38	theme	Magnetite	263:271	arg1	NCs					348:350	NCs	348:350	NCs	348:350	Magnetite (Fe3O4) nanoparticles (NPs) as well as chitosan (CS)/Fe3O4 nanocomposites (NCs) and graphene(Gr)/CS/Fe3O4 NCs were synthesized by simple hydrothermal method.					
33721803	3	39	dep	aeruginosa	592:601	arg1	strains					631:637	bacterial strains	621:637	bacterial strains	621:637	Their composition, structure and morphology were studied, followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains.					
33721803	4	40	theme	antibacterial	688:700	arg1	activity					702:709	significantly higher antibacterial activity	667:709	significantly higher antibacterial activity	667:709	The Gr/CS/Fe3O4 NCs showed significantly higher antibacterial activity compared to Fe3O4 NPs and CS/Fe3O4 NCs (105 and 69 % higher against P. aeruginosa as well as 91 and 77 % higher against K. pneumoniae, respectively).					
33721803	3	41	dep	studied	480:486	arg1	followed					489:496	followed	489:496	followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains	489:637	Their composition, structure and morphology were studied, followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains.					
33721803	6	42	theme	Gr/CS/Fe3O4	1062:1072	arg1	NCs					1074:1076	Gr/CS/Fe3O4 NCs	1062:1076	Gr/CS/Fe3O4 NCs	1062:1076	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	3	43	theme	antibacterial	525:537	arg1	activity					539:546	their antibacterial activity	519:546	their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains	519:637	Their composition, structure and morphology were studied, followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains.					
33721803	5	44	theme	inhibitory	873:882	arg1	MIC					899:901	MIC	899:901	MIC	899:901	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	5	44	theme	inhibitory	873:882	arg1	concentration					884:896	The minimum inhibitory concentration	861:896	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae	861:961	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	5	44	theme	inhibitory	873:882	arg1	μg/mL					978:982	60 and 70 μg/mL	968:982	60 and 70 μg/mL	968:982	The minimum inhibitory concentration (MIC) of Gr/CS/Fe3O4 NCs against P. aeruginosa and K. pneumoniae were 60 and 70 μg/mL, respectively.					
33721803	6	45	theme	antibacterial	1015:1027	arg1	activity					1029:1036	synergistic antibacterial activity	1003:1036	synergistic antibacterial activity	1003:1036	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	6	46	with	agents	1147:1152	arg1	potential					1159:1167	potential	1159:1167	potential for a wide range of biomedical applications, where antibacterial properties are needed	1159:1254	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33721803	3	47	theme	activity	539:546	arg1	evaluation					505:514	the evaluation	501:514	the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains	501:637	Their composition, structure and morphology were studied, followed by the evaluation of their antibacterial activity against ESBL-producing and gram-negative P. aeruginosa and K. pneumoniae bacterial strains.					
33721803	6	48	theme	synergistic	1003:1013	arg1	activity					1029:1036	synergistic antibacterial activity	1003:1036	synergistic antibacterial activity	1003:1036	The synergistic antibacterial activity and facile synthesis of Gr/CS/Fe3O4 NCs suggests their applicability as novel highly efficient antibacterial agents with potential for a wide range of biomedical applications, where antibacterial properties are needed.					
33427929	0	0	theme	substrate	89:97	arg1	preferences					99:109	altered substrate preferences	81:109	altered substrate preferences	81:109	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	7	1	theme	molecular	1199:1207	arg1	weight					1209:1214	low molecular weight	1195:1214	low molecular weight	1195:1214	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	7	2	theme	evolved	1002:1008	arg1	enzymes					1010:1016	the evolved enzymes	998:1016	the evolved enzymes	998:1016	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	0	3	theme	altered	81:87	arg1	preferences					99:109	altered substrate preferences	81:109	altered substrate preferences	81:109	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	7	4	from	°C	1153:1154	arg1	active					1140:1145	active	1140:1145	active	1140:1145	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	2	5	theme	desired	365:371	arg1	function					373:380	the desired function	361:380	the desired function of the endoglucanase	361:401	In all of these applications, the desired function of the endoglucanase is to create nicks in the amorphous regions of the cellulose.					
33427929	1	6	theme	composite	277:285	arg1	materials					287:295	composite materials	277:295	composite materials	277:295	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	5	7	theme	thermostable	736:747	arg1	thermophila					771:781	a thermostable bacterium Spirochaeta thermophila	734:781	a thermostable bacterium Spirochaeta thermophila	734:781	To this end, a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila was for the first time cloned and characterized.					
33427929	11	8	theme	fiber	1651:1655	arg1	modification					1657:1668	cellulose fiber modification	1641:1668	cellulose fiber modification	1641:1668	• A mutant with a preference for long molecular weight substrate was obtained and proposed to be beneficial for cellulose fiber modification.					
33427929	7	9	dep	little	1168:1173	arg1	substrates					1216:1225	substrates	1216:1225	substrates	1216:1225	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	3	10	from	activity	513:520	arg1	fibers					529:534	the fibers	525:534	the fibers	525:534	However, endoglucanase can be diverted from its activity on the fibers by other substrates-soluble oligosaccharides.					
33427929	3	11	theme	other	539:543	arg1	oligosaccharides					564:579	other substrates-soluble oligosaccharides	539:579	other substrates-soluble oligosaccharides	539:579	However, endoglucanase can be diverted from its activity on the fibers by other substrates-soluble oligosaccharides.					
33427929	8	12	from	instrumental	1250:1261	arg1	biomaterials					1325:1336	biomaterials	1325:1336	biomaterials	1325:1336	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	8	12	from	instrumental	1250:1261	arg1	biofuels					1312:1319	second-generation biofuels	1294:1319	second-generation biofuels	1294:1319	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	8	12	from	instrumental	1250:1261	arg1	sectors					1277:1283	bioeconomy sectors	1266:1283	bioeconomy sectors	1266:1283	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	5	13	theme	bacterium	749:757	arg1	thermophila					771:781	a thermostable bacterium Spirochaeta thermophila	734:781	a thermostable bacterium Spirochaeta thermophila	734:781	To this end, a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila was for the first time cloned and characterized.					
33427929	8	14	theme	lignocellulosic	1343:1357	arg1	biomass					1359:1365	lignocellulosic biomass	1343:1365	lignocellulosic biomass	1343:1365	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	3	15	theme	substrates-soluble	545:562	arg1	oligosaccharides					564:579	other substrates-soluble oligosaccharides	539:579	other substrates-soluble oligosaccharides	539:579	However, endoglucanase can be diverted from its activity on the fibers by other substrates-soluble oligosaccharides.					
33427929	4	16	theme	enzyme	660:665	arg1	approach					677:684	an enzyme evolution approach	657:684	an enzyme evolution approach	657:684	This issue was addressed in the current study using enzyme engineering and an enzyme evolution approach.					
33427929	5	17	theme	Spirochaeta	759:769	arg1	thermophila					771:781	a thermostable bacterium Spirochaeta thermophila	734:781	a thermostable bacterium Spirochaeta thermophila	734:781	To this end, a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila was for the first time cloned and characterized.					
33427929	0	18	with	mutants	68:74	arg1	preferences					99:109	altered substrate preferences	81:109	altered substrate preferences	81:109	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	0	19	from	thermophila	48:58	arg1	endoglucanase					17:29	New thermostable endoglucanase	0:29	New thermostable endoglucanase from Spirochaeta thermophila	0:58	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	0	19	from	thermophila	48:58	arg1	mutants					68:74	its mutants	64:74	its mutants with altered substrate preferences	64:109	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	11	20	theme	weight	1577:1582	arg1	substrate					1584:1592	long molecular weight substrate	1562:1592	long molecular weight substrate	1562:1592	• A mutant with a preference for long molecular weight substrate was obtained and proposed to be beneficial for cellulose fiber modification.					
33427929	6	21	used	used	857:860	arg2	enzyme					846:851	The wild-type enzyme	832:851	The wild-type enzyme	832:851	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	6	21	used	used	857:860	arg2	point					876:880	a starting point	865:880	a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates	865:983	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	1	22	theme	cellulosic	188:197	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	22	theme	cellulosic	188:197	arg1	modification					235:246	cellulose fiber modification	219:246	cellulose fiber modification for the production paper and composite materials	219:295	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	0	23	theme	thermostable	4:15	arg1	endoglucanase					17:29	New thermostable endoglucanase	0:29	New thermostable endoglucanase from Spirochaeta thermophila	0:58	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	5	24	theme	first	795:799	arg1	time					801:804	the first time	791:804	the first time	791:804	To this end, a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila was for the first time cloned and characterized.					
33427929	7	25	with	activity	1181:1188	arg1	weight					1209:1214	low molecular weight	1195:1214	low molecular weight	1195:1214	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	1	26	theme	biomass	199:205	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	26	theme	biomass	199:205	arg1	modification					235:246	cellulose fiber modification	219:246	cellulose fiber modification for the production paper and composite materials	219:295	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	0	27	theme	New	0:2	arg1	endoglucanase					17:29	New thermostable endoglucanase	0:29	New thermostable endoglucanase from Spirochaeta thermophila	0:58	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	7	28	from	temperatures	1102:1113	arg1	active					1027:1032	active	1027:1032	active	1027:1032	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	7	28	from	temperatures	1102:1113	arg1	best					990:993	best	990:993	best	990:993	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	10	29	theme	endoglucanase	1470:1482	arg1	specificity					1450:1460	The substrate specificity	1436:1460	The substrate specificity of this endoglucanase	1436:1482	• The substrate specificity of this endoglucanase was changed by means of genetic engineering.					
33427929	11	30	theme	long	1562:1565	arg1	weight					1577:1582	long molecular weight	1562:1582	long molecular weight substrate	1562:1592	• A mutant with a preference for long molecular weight substrate was obtained and proposed to be beneficial for cellulose fiber modification.					
33427929	11	31	theme	cellulose	1641:1649	arg1	modification					1657:1668	cellulose fiber modification	1641:1668	cellulose fiber modification	1641:1668	• A mutant with a preference for long molecular weight substrate was obtained and proposed to be beneficial for cellulose fiber modification.					
33427929	2	32	theme	endoglucanase	389:401	arg1	function					373:380	the desired function	361:380	the desired function of the endoglucanase	361:401	In all of these applications, the desired function of the endoglucanase is to create nicks in the amorphous regions of the cellulose.					
33427929	5	33	theme	hypothetical	702:713	arg1	endoglucanase					715:727	a hypothetical endoglucanase	700:727	a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila	700:781	To this end, a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila was for the first time cloned and characterized.					
33427929	1	34	from	elements	135:142	arg1	applications					166:177	several industrial applications	147:177	several industrial applications	147:177	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	34	from	elements	135:142	arg1	production					319:328	nanocellulose production	305:328	nanocellulose production	305:328	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	34	from	elements	135:142	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	2	35	theme	amorphous	429:437	arg1	regions					439:445	the amorphous regions	425:445	the amorphous regions of the cellulose	425:462	In all of these applications, the desired function of the endoglucanase is to create nicks in the amorphous regions of the cellulose.					
33427929	1	36	theme	nanocellulose	305:317	arg1	production					319:328	nanocellulose production	305:328	nanocellulose production	305:328	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	10	37	theme	genetic	1508:1514	arg1	engineering					1516:1526	genetic engineering	1508:1526	genetic engineering	1508:1526	• The substrate specificity of this endoglucanase was changed by means of genetic engineering.					
33427929	1	38	theme	cellulose	219:227	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	38	theme	cellulose	219:227	arg1	modification					235:246	cellulose fiber modification	219:246	cellulose fiber modification for the production paper and composite materials	219:295	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	6	39	theme	molecular	902:910	arg1	evolution					912:920	molecular evolution	902:920	molecular evolution	902:920	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	7	40	theme	molecular	1072:1080	arg1	weight					1082:1087	high molecular weight	1067:1087	high molecular weight substrate	1067:1097	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	1	41	dep	paper	267:271	arg1	the					252:254	the	252:254	the	252:254	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	9	42	dep	POINTS	1372:1377	arg1	characterized					1419:1431	characterized	1419:1431	was characterized	1415:1431	KEY POINTS: • A new thermostable endoglucanase was characterized.					
33427929	1	43	theme	fiber	229:233	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	43	theme	fiber	229:233	arg1	modification					235:246	cellulose fiber modification	219:246	cellulose fiber modification for the production paper and composite materials	219:295	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	0	44	theme	Spirochaeta	36:46	arg1	thermophila					48:58	Spirochaeta thermophila	36:58	Spirochaeta thermophila	36:58	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	7	45	theme	weight	1082:1087	arg1	substrate					1089:1097	high molecular weight substrate	1067:1097	high molecular weight substrate	1067:1097	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	4	46	theme	enzyme	634:639	arg1	engineering					641:651	enzyme engineering	634:651	enzyme engineering	634:651	This issue was addressed in the current study using enzyme engineering and an enzyme evolution approach.					
33427929	8	47	theme	bioeconomy	1266:1275	arg1	biomaterials					1325:1336	biomaterials	1325:1336	biomaterials	1325:1336	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	8	47	theme	bioeconomy	1266:1275	arg1	biofuels					1312:1319	second-generation biofuels	1294:1319	second-generation biofuels	1294:1319	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	8	47	theme	bioeconomy	1266:1275	arg1	sectors					1277:1283	bioeconomy sectors	1266:1283	bioeconomy sectors	1266:1283	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	10	48	theme	substrate	1440:1448	arg1	specificity					1450:1460	The substrate specificity	1436:1460	The substrate specificity of this endoglucanase	1436:1482	• The substrate specificity of this endoglucanase was changed by means of genetic engineering.					
33427929	6	49	theme	weight	967:972	arg1	substrates					974:983	the higher molecular weight substrates	946:983	the higher molecular weight substrates	946:983	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	5	50	from	thermophila	771:781	arg1	endoglucanase					715:727	a hypothetical endoglucanase	700:727	a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila	700:781	To this end, a hypothetical endoglucanase from a thermostable bacterium Spirochaeta thermophila was for the first time cloned and characterized.					
33427929	6	51	theme	starting	867:874	arg1	point					876:880	a starting point	865:880	a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates	865:983	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	6	51	theme	starting	867:874	arg1	enzyme					846:851	The wild-type enzyme	832:851	The wild-type enzyme	832:851	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	0	52	with	endoglucanase	17:29	arg1	preferences					99:109	altered substrate preferences	81:109	altered substrate preferences	81:109	New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences.					
33427929	1	53	theme	key	131:133	arg1	Endoglucanases					112:125	Endoglucanases	112:125	Endoglucanases	112:125	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	53	theme	key	131:133	arg1	elements					135:142	key elements	131:142	key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production	131:328	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	7	54	dep	°C	1124:1125	arg1	active					1140:1145	active	1140:1145	active	1140:1145	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	6	55	theme	molecular	957:965	arg1	weight					967:972	higher molecular weight	950:972	the higher molecular weight substrates	946:983	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	7	56	theme	high	1067:1070	arg1	weight					1082:1087	high molecular weight	1067:1087	high molecular weight substrate	1067:1097	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	6	57	theme	higher	950:955	arg1	weight					967:972	higher molecular weight	950:972	the higher molecular weight substrates	946:983	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	7	58	theme	wild-type	1043:1051	arg1	enzyme					1053:1058	the wild-type enzyme	1039:1058	the wild-type enzyme	1039:1058	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
33427929	9	59	theme	KEY	1368:1370	arg1	POINTS					1372:1377	KEY POINTS	1368:1377	KEY POINTS: • A new thermostable endoglucanase was characterized.	1368:1432	KEY POINTS: • A new thermostable endoglucanase was characterized.					
33427929	6	60	theme	wild-type	836:844	arg1	point					876:880	a starting point	865:880	a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates	865:983	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	6	60	theme	wild-type	836:844	arg1	enzyme					846:851	The wild-type enzyme	832:851	The wild-type enzyme	832:851	The wild-type enzyme was used as a starting point for mutagenesis and molecular evolution toward a preference for the higher molecular weight substrates.					
33427929	8	61	theme	second-generation	1294:1310	arg1	biofuels					1312:1319	second-generation biofuels	1294:1319	second-generation biofuels	1294:1319	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	11	62	theme	molecular	1567:1575	arg1	weight					1577:1582	long molecular weight	1562:1582	long molecular weight substrate	1562:1592	• A mutant with a preference for long molecular weight substrate was obtained and proposed to be beneficial for cellulose fiber modification.					
33427929	9	63	theme	new	1384:1386	arg1	endoglucanase					1401:1413	• A new thermostable endoglucanase	1380:1413	• A new thermostable endoglucanase	1380:1413	KEY POINTS: • A new thermostable endoglucanase was characterized.					
33427929	9	64	theme	thermostable	1388:1399	arg1	endoglucanase					1401:1413	• A new thermostable endoglucanase	1380:1413	• A new thermostable endoglucanase	1380:1413	KEY POINTS: • A new thermostable endoglucanase was characterized.					
33427929	8	65	from	sectors	1277:1283	arg1	instrumental					1250:1261	instrumental	1250:1261	instrumental	1250:1261	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	1	66	theme	several	147:153	arg1	applications					166:177	several industrial applications	147:177	several industrial applications	147:177	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	66	theme	several	147:153	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	8	67	from	biomass	1359:1365	arg1	biomaterials					1325:1336	biomaterials	1325:1336	biomaterials	1325:1336	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	8	67	from	biomass	1359:1365	arg1	biofuels					1312:1319	second-generation biofuels	1294:1319	second-generation biofuels	1294:1319	These findings can be instrumental in bioeconomy sectors, such as second-generation biofuels and biomaterials from lignocellulosic biomass.					
33427929	4	68	theme	current	614:620	arg1	study					622:626	the current study	610:626	the current study using enzyme engineering and an enzyme evolution approach	610:684	This issue was addressed in the current study using enzyme engineering and an enzyme evolution approach.					
33427929	11	69	with	mutant	1533:1538	arg1	preference					1547:1556	a preference	1545:1556	a preference for long molecular weight substrate	1545:1592	• A mutant with a preference for long molecular weight substrate was obtained and proposed to be beneficial for cellulose fiber modification.					
33427929	1	70	theme	industrial	155:164	arg1	applications					166:177	several industrial applications	147:177	several industrial applications	147:177	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	1	70	theme	industrial	155:164	arg1	hydrolysis					207:216	cellulosic biomass hydrolysis	188:216	cellulosic biomass hydrolysis	188:216	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	4	71	theme	evolution	667:675	arg1	approach					677:684	an enzyme evolution approach	657:684	an enzyme evolution approach	657:684	This issue was addressed in the current study using enzyme engineering and an enzyme evolution approach.					
33427929	1	72	theme	production	256:265	arg1	paper					267:271	production paper	256:271	production paper	256:271	Endoglucanases are key elements in several industrial applications, such as cellulosic biomass hydrolysis, cellulose fiber modification for the production paper and composite materials, and in nanocellulose production.					
33427929	2	73	theme	cellulose	454:462	arg1	regions					439:445	the amorphous regions	425:445	the amorphous regions of the cellulose	425:462	In all of these applications, the desired function of the endoglucanase is to create nicks in the amorphous regions of the cellulose.					
33427929	7	74	theme	low	1195:1197	arg1	weight					1209:1214	low molecular weight	1195:1214	low molecular weight	1195:1214	The best of the evolved enzymes was more active than the wild-type enzyme toward high molecular weight substrate at temperatures below 45 °C (3-fold more active at 30 °C) and showed little or no activity with low molecular weight substrates.					
32942686	0	0	theme	Dietary	78:84	arg1	Intake					92:97	Estimating Dietary Fiber Intake	67:97	Estimating Dietary Fiber Intake	67:97	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	3	1	theme	inulin-type	632:642	arg1	fructans					644:651	inulin-type fructans	632:651	inulin-type fructans	632:651	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	3	2	from	products	716:723	arg1	DF					553:554	DF	553:554	DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe	553:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	3	2	from	products	716:723	arg1	repertoire					539:548	A repertoire	537:548	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe	537:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	0	3	theme	Estimating	67:76	arg1	Intake					92:97	Estimating Dietary Fiber Intake	67:97	Estimating Dietary Fiber Intake	67:97	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	1	4	theme	Most	156:159	arg1	tables					187:192	Most official food composition tables	156:192	Most official food composition tables	156:192	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	7	5	theme	total	1224:1228	arg1	DF					1240:1241	DF	1240:1241	DF including prebiotics	1240:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	7	5	theme	total	1224:1228	arg1	amount					1230:1235	the total amount	1220:1235	the total amount of DF including prebiotics	1220:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	2	6	theme	intake	479:484	arg1	estimation					486:495	detailed DF intake estimation	467:495	detailed DF intake estimation including prebiotic (oligo)saccharides	467:534	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	5	7	theme	tested	912:917	arg1	population					919:928	the tested population	908:928	the tested population	908:928	Our data report a total DF intake of 38 g/day in the tested population.					
32942686	1	8	theme	fermentable	255:265	arg1	DF					283:284	DF	283:284	DF	283:284	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	1	8	theme	fermentable	255:265	arg1	fibers					275:280	fermentable dietary fibers	255:280	fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota	255:366	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	0	9	theme	Fiber	86:90	arg1	Intake					92:97	Estimating Dietary Fiber Intake	67:97	Estimating Dietary Fiber Intake	67:97	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	4	10	theme	healthy	815:821	arg1	volunteers					823:832	15 healthy volunteers	812:832	15 healthy volunteers	812:832	A 12 month FFQ was developed and submitted to 15 healthy volunteers from the FiberTAG study.					
32942686	0	11	dep	Considering	99:109	arg1	Input					123:127	Input	123:127	Input from the FiberTAG Project	123:153	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	8	12	from	intervention	1293:1304	arg1	helpful					1282:1288	helpful	1282:1288	helpful	1282:1288	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	1	13	theme	dietary	267:273	arg1	DF					283:284	DF	283:284	DF	283:284	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	1	13	theme	dietary	267:273	arg1	fibers					275:280	fermentable dietary fibers	255:280	fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota	255:366	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	2	14	theme	detailed	467:474	arg1	DF					476:477	detailed DF	467:477	detailed DF intake estimation including prebiotic (oligo)saccharides	467:534	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	2	15	theme	study	385:389	arg1	aim					373:375	The aim	369:375	The aim of this study	369:389	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	5	16	theme	g/day	899:903	arg1	intake					886:891	a total DF intake	875:891	a total DF intake of 38 g/day in the tested population	875:928	Our data report a total DF intake of 38 g/day in the tested population.					
32942686	1	17	theme	official	161:168	arg1	tables					187:192	Most official food composition tables	156:192	Most official food composition tables	156:192	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	2	18	dep	prebiotic	507:515	arg1	oligo					518:522	oligo	518:522	oligo	518:522	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	8	19	theme	intake	1444:1449	arg1	recommendations					1451:1465	DF intake recommendations	1441:1465	DF intake recommendations	1441:1465	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	6	20	theme	galacto-oligosaccharides	1072:1095	arg1	level					1104:1108	galacto-oligosaccharides intake level	1072:1108	galacto-oligosaccharides intake level	1072:1108	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	8	21	from	helpful	1282:1288	arg1	intervention					1293:1304	intervention	1293:1304	intervention	1293:1304	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	8	21	from	helpful	1282:1288	arg1	studies					1323:1329	observational studies	1309:1329	observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF	1309:1519	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	1	22	theme	food	170:173	arg1	tables					187:192	Most official food composition tables	156:192	Most official food composition tables	156:192	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	1	23	theme	gut	353:355	arg1	microbiota					357:366	the gut microbiota	349:366	the gut microbiota	349:366	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	1	24	theme	composition	175:185	arg1	tables					187:192	Most official food composition tables	156:192	Most official food composition tables	156:192	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	8	25	theme	pathological	1396:1407	arg1	contexts					1409:1416	different pathological contexts	1386:1416	different pathological contexts	1386:1416	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	5	26	theme	total	877:881	arg1	intake					886:891	a total DF intake	875:891	a total DF intake of 38 g/day in the tested population	875:928	Our data report a total DF intake of 38 g/day in the tested population.					
32942686	6	27	theme	condiments	993:1002	arg1	ingestion					1024:1032	condiments (garlic and onions) ingestion	993:1032	condiments (garlic and onions) ingestion	993:1032	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	0	28	theme	Repertoire	17:26	arg1	Development					0:10	Development	0:10	Development of a Repertoire	0:26	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	0	28	theme	Repertoire	17:26	arg1	Questionnaire					49:61	a Food Frequency Questionnaire	32:61	a Food Frequency Questionnaire for Estimating Dietary Fiber Intake	32:97	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	3	29	theme	food	711:714	arg1	products					716:723	food products	711:723	food products consumed in Europe	711:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	8	30	theme	microbiota-nutrient	1350:1368	arg1	interactions					1370:1381	microbiota-nutrient interactions	1350:1381	microbiota-nutrient interactions in different pathological contexts	1350:1416	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	5	31	theme	DF	883:884	arg1	intake					886:891	a total DF intake	875:891	a total DF intake of 38 g/day in the tested population	875:928	Our data report a total DF intake of 38 g/day in the tested population.					
32942686	3	32	from	repertoire	539:548	arg1	products					716:723	food products	711:723	food products consumed in Europe	711:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	6	33	dep	condiments	993:1002	arg1	onions					1016:1021	onions	1016:1021	onions	1016:1021	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	6	33	dep	condiments	993:1002	arg1	garlic					1005:1010	garlic	1005:1010	garlic	1005:1010	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	2	34	theme	prebiotic	507:515	arg1	saccharides					524:534	prebiotic (oligo)saccharides	507:534	prebiotic (oligo)saccharides	507:534	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	3	35	theme	insoluble	585:593	arg1	DF					595:596	insoluble DF	585:596	insoluble DF	585:596	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	1	36	theme	food	198:201	arg1	questionnaires					203:216	food questionnaires	198:216	food questionnaires	198:216	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	2	37	theme	frequency	428:436	arg1	FFQ					453:455	FFQ	453:455	FFQ	453:455	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	2	37	theme	frequency	428:436	arg1	questionnaire					438:450	a food frequency questionnaire	421:450	a food frequency questionnaire (FFQ)	421:456	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	7	38	theme	major	1186:1190	arg1	FFQ					1178:1180	FFQ	1178:1180	FFQ	1178:1180	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	7	38	theme	major	1186:1190	arg1	tools					1192:1196	major tools	1186:1196	major tools for the evaluation of the total amount of DF including prebiotics	1186:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	7	38	theme	major	1186:1190	arg1	repertoire					1163:1172	the FiberTAG repertoire	1150:1172	the FiberTAG repertoire	1150:1172	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	4	39	theme	12	768:769	arg1	month					771:775	month	771:775	month	771:775	A 12 month FFQ was developed and submitted to 15 healthy volunteers from the FiberTAG study.					
32942686	3	40	from	DF	553:554	arg1	products					716:723	food products	711:723	food products consumed in Europe	711:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	2	41	theme	food	423:426	arg1	FFQ					453:455	FFQ	453:455	FFQ	453:455	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	2	41	theme	food	423:426	arg1	questionnaire					438:450	a food frequency questionnaire	421:450	a food frequency questionnaire (FFQ)	421:456	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
32942686	8	42	theme	lifestyles	1486:1495	arg1	part					1470:1473	part	1470:1473	part of healthy lifestyles considering specific DF	1470:1519	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	6	43	attach	linked	975:980	arg1	ingestion					1024:1032	condiments (garlic and onions) ingestion	993:1032	condiments (garlic and onions) ingestion	993:1032	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	6	43	attach	linked	975:980	arg2	Fructan					931:937	Fructan	931:937	Fructan	931:937	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	3	44	theme	DF	553:554	arg1	DF					553:554	DF	553:554	DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe	553:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	3	44	theme	DF	553:554	arg1	repertoire					539:548	A repertoire	537:548	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe	537:742	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	0	45	theme	Frequency	39:47	arg1	Questionnaire					49:61	a Food Frequency Questionnaire	32:61	a Food Frequency Questionnaire for Estimating Dietary Fiber Intake	32:97	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	8	46	theme	healthy	1478:1484	arg1	lifestyles					1486:1495	healthy lifestyles	1478:1495	healthy lifestyles considering specific DF	1478:1519	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	3	47	dep	saccharides	619:629	arg1	galacto-oligosaccharides					682:705	galacto-oligosaccharides	682:705	galacto-oligosaccharides	682:705	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	3	47	dep	saccharides	619:629	arg1	fructo-oligosaccharides					654:676	fructo-oligosaccharides	654:676	fructo-oligosaccharides	654:676	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	3	47	dep	saccharides	619:629	arg1	fructans					644:651	inulin-type fructans	632:651	inulin-type fructans	632:651	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	0	48	theme	Food	34:37	arg1	Questionnaire					49:61	a Food Frequency Questionnaire	32:61	a Food Frequency Questionnaire for Estimating Dietary Fiber Intake	32:97	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	8	49	theme	specific	1509:1516	arg1	DF					1518:1519	specific DF	1509:1519	specific DF	1509:1519	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	0	50	theme	FiberTAG	138:145	arg1	Project					147:153	the FiberTAG Project	134:153	the FiberTAG Project	134:153	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	8	51	theme	different	1386:1394	arg1	contexts					1409:1416	different pathological contexts	1386:1416	different pathological contexts	1386:1416	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	4	52	theme	month	771:775	arg1	FFQ					777:779	A 12 month FFQ	766:779	A 12 month FFQ	766:779	A 12 month FFQ was developed and submitted to 15 healthy volunteers from the FiberTAG study.					
32942686	7	53	theme	amount	1230:1235	arg1	evaluation					1206:1215	the evaluation	1202:1215	the evaluation of the total amount of DF including prebiotics	1202:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	6	54	dep	lower	1116:1120	arg1	g/day					1125:1129	1 g/day	1123:1129	1 g/day	1123:1129	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	3	55	theme	oligo	613:617	arg1	saccharides					619:629	prebiotic (oligo)saccharides	602:629	prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides)	602:706	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	8	56	theme	observational	1309:1321	arg1	studies					1323:1329	observational studies	1309:1329	observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF	1309:1519	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	5	57	from	intake	886:891	arg1	population					919:928	the tested population	908:928	the tested population	908:928	Our data report a total DF intake of 38 g/day in the tested population.					
32942686	4	58	theme	FiberTAG	843:850	arg1	study					852:856	the FiberTAG study	839:856	the FiberTAG study	839:856	A 12 month FFQ was developed and submitted to 15 healthy volunteers from the FiberTAG study.					
32942686	3	59	theme	soluble	573:579	arg1	DF					581:582	soluble DF	573:582	soluble DF	573:582	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	8	60	from	studies	1323:1329	arg1	helpful					1282:1288	helpful	1282:1288	helpful	1282:1288	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	1	61	with	interaction	332:342	arg1	microbiota					357:366	the gut microbiota	349:366	the gut microbiota	349:366	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	6	62	theme	fructo-oligosaccharides	943:965	arg1	intake					967:972	fructo-oligosaccharides intake	943:972	fructo-oligosaccharides intake	943:972	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	8	63	from	interactions	1370:1381	arg1	contexts					1409:1416	different pathological contexts	1386:1416	different pathological contexts	1386:1416	Their use can be helpful in intervention or observational studies devoted to analyze microbiota-nutrient interactions in different pathological contexts, as well as to revisit DF intake recommendations as part of healthy lifestyles considering specific DF.					
32942686	3	64	theme	prebiotic	602:610	arg1	saccharides					619:629	prebiotic (oligo)saccharides	602:629	prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides)	602:706	A repertoire of DF detailing total, soluble DF, insoluble DF and prebiotic (oligo)saccharides (inulin-type fructans, fructo-oligosaccharides and galacto-oligosaccharides) in food products consumed in Europe has been established.					
32942686	7	65	theme	FiberTAG	1154:1161	arg1	FFQ					1178:1180	FFQ	1178:1180	FFQ	1178:1180	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	7	65	theme	FiberTAG	1154:1161	arg1	tools					1192:1196	major tools	1186:1196	major tools for the evaluation of the total amount of DF including prebiotics	1186:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	7	65	theme	FiberTAG	1154:1161	arg1	repertoire					1163:1172	the FiberTAG repertoire	1150:1172	the FiberTAG repertoire	1150:1172	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	6	66	theme	intake	1097:1102	arg1	level					1104:1108	galacto-oligosaccharides intake level	1072:1108	galacto-oligosaccharides intake level	1072:1108	Fructan and fructo-oligosaccharides intake, linked notably to condiments (garlic and onions) ingestion, reached 5 and 2 g/day, respectively, galacto-oligosaccharides intake level being lower (1 g/day).					
32942686	0	67	from	Project	147:153	arg1	Input					123:127	Input	123:127	Input from the FiberTAG Project	123:153	Development of a Repertoire and a Food Frequency Questionnaire for Estimating Dietary Fiber Intake Considering Prebiotics: Input from the FiberTAG Project.					
32942686	1	68	theme	health	304:309	arg1	effect					311:316	a health effect	302:316	a health effect	302:316	Most official food composition tables and food questionnaires do not provide enough data to assess fermentable dietary fibers (DF) that can exert a health effect through their interaction with the gut microbiota.					
32942686	7	69	theme	DF	1240:1241	arg1	DF					1240:1241	DF	1240:1241	DF including prebiotics	1240:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	7	69	theme	DF	1240:1241	arg1	amount					1230:1235	the total amount	1220:1235	the total amount of DF including prebiotics	1220:1262	We conclude that the FiberTAG repertoire and FFQ are major tools for the evaluation of the total amount of DF including prebiotics.					
32942686	2	70	theme	DF	476:477	arg1	estimation					486:495	detailed DF intake estimation	467:495	detailed DF intake estimation including prebiotic (oligo)saccharides	467:534	The aim of this study was to develop a database and a food frequency questionnaire (FFQ) allowing detailed DF intake estimation including prebiotic (oligo)saccharides.					
34836688	0	0	theme	alginate	67:74	arg1	beads					76:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	6	1	dep	reusability	1101:1111	arg1	%					1133:1133	efficiency of 95.25%	1114:1133	efficiency of 95.25% after four runs	1114:1149	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	10	2	dep	Cr	1710:1711	arg1	soil					1740:1743	soil	1740:1743	soil	1740:1743	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	10	2	dep	Cr	1710:1711	arg1	VI					1713:1714	VI	1713:1714	VI	1713:1714	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	10	2	dep	Cr	1710:1711	arg1	water					1730:1734	water	1730:1734	water	1730:1734	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	1	3	theme	Efficient	136:144	arg1	Cr					167:168	Cr	167:168	Cr	167:168	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	1	3	theme	Efficient	136:144	arg1	chromium					157:164	Efficient nano-scale chromium	136:164	Efficient nano-scale chromium (Cr) remediating agents used in the water industry	136:215	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	0	4	theme	alcohol/sodium	52:65	arg1	beads					76:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	5	5	dep	process	1008:1014	arg1	VI					993:994	VI	993:994	VI	993:994	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	6	6	theme	of	1125:1126	arg1	%					1133:1133	efficiency of 95.25%	1114:1133	efficiency of 95.25% after four runs	1114:1149	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	0	7	attach	Removal	0:6	arg1	remediation					123:133	soil remediation	118:133	soil remediation	118:133	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	0	7	attach	Removal	0:6	arg2	Cr					11:12	Cr	11:12	Cr(VI)	11:16	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	1	8	theme	nano-scale	146:155	arg1	Cr					167:168	Cr	167:168	Cr	167:168	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	1	8	theme	nano-scale	146:155	arg1	chromium					157:164	Efficient nano-scale chromium	136:164	Efficient nano-scale chromium (Cr) remediating agents used in the water industry	136:215	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	7	9	theme	contaminated	1308:1319	arg1	soil					1321:1324	the contaminated soil	1304:1324	the contaminated soil after 30 days	1304:1338	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	2	10	theme	gel	464:466	arg1	beads					468:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	3	11	theme	remediation	587:597	arg1	impacts					599:605	the remediation impacts	583:605	the remediation impacts	583:605	Additionally, the PPS was used to recover hexavalent chromium (Cr(VI)) contaminated water and soil to study the remediation impacts and mechanism.					
34836688	6	12	theme	efficiency	1114:1123	arg1	%					1133:1133	efficiency of 95.25%	1114:1133	efficiency of 95.25% after four runs	1114:1149	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	6	13	theme	<	1074:1074	arg1	release					1088:1094	< 13 mg/L TOC release	1074:1094	< 13 mg/L TOC release	1074:1094	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	6	13	theme	<	1074:1074	arg1	properties					1062:1071	excellent physicochemical properties	1036:1071	excellent physicochemical properties (< 13 mg/L TOC release)	1036:1095	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	4	14	theme	irregular	657:665	arg1	PPS					646:648	the PPS	642:648	the PPS	642:648	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	4	14	theme	irregular	657:665	arg1	sphere					667:672	an irregular sphere	654:672	an irregular sphere with a pore size of 24.24 nm	654:701	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	6	15	theme	TOC	1084:1086	arg1	release					1088:1094	< 13 mg/L TOC release	1074:1094	< 13 mg/L TOC release	1074:1094	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	6	15	theme	TOC	1084:1086	arg1	properties					1062:1071	excellent physicochemical properties	1036:1071	excellent physicochemical properties (< 13 mg/L TOC release)	1036:1095	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	7	16	theme	Cr	1294:1295	arg1	%					1266:1266	24.17%	1261:1266	24.17% of total Cr	1261:1278	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	16	theme	Cr	1294:1295	arg1	Cr					1277:1278	total Cr	1271:1278	total Cr	1271:1278	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	16	theme	Cr	1294:1295	arg1	%					1289:1289	52.47%	1284:1289	52.47% of Cr	1284:1295	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	16	theme	Cr	1294:1295	arg1	Cr					1294:1295	Cr	1294:1295	Cr	1294:1295	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	6	17	theme	physicochemical	1046:1060	arg1	release					1088:1094	< 13 mg/L TOC release	1074:1094	< 13 mg/L TOC release	1074:1094	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	6	17	theme	physicochemical	1046:1060	arg1	properties					1062:1071	excellent physicochemical properties	1036:1071	excellent physicochemical properties (< 13 mg/L TOC release)	1036:1095	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	9	18	dep	proceeded	1548:1556	arg1	VI					1513:1514	VI	1513:1514	VI	1513:1514	Elemental speciation by XPS revealed that Cr(VI) removal from solution and soil proceeded mainly by electrostatic attraction, reduction, and complexation/chelation.					
34836688	5	19	dep	Cr	790:791	arg1	adsorption					797:806	adsorption	797:806	The Cr(VI) adsorption by PPS	786:813	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	5	19	dep	Cr	790:791	arg1	VI					793:794	VI	793:794	VI	793:794	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	2	20	theme	SA	460:461	arg1	beads					468:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	9	21	from	solution	1530:1537	arg1	removal					1517:1523	removal	1517:1523	removal from solution and soil	1517:1546	Elemental speciation by XPS revealed that Cr(VI) removal from solution and soil proceeded mainly by electrostatic attraction, reduction, and complexation/chelation.					
34836688	7	22	theme	PPS	1215:1217	arg1	treatment					1226:1234	the 5% PPS (5PPS) treatment	1208:1234	the 5% PPS (5PPS) treatment	1208:1234	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	5	23	theme	chemical	938:945	arg1	reactions					947:955	the chemical reactions	934:955	the chemical reactions	934:955	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	5	23	theme	chemical	938:945	arg1	step					978:981	the controlling step	962:981	the controlling step in the Cr(VI) adsorption process	962:1014	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	4	24	theme	adsorption	724:733	arg1	83.1 mg/g					745:753	83.1 mg/g	745:753	83.1 mg/g	745:753	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	4	24	theme	adsorption	724:733	arg1	capacity					735:742	strong adsorption capacity	717:742	strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water	717:783	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	8	25	from	%	1422:1422	arg1	group					1447:1451	the 5PPS group	1438:1451	the 5PPS group	1438:1451	Meanwhile, the water-soluble and the leaching Cr contents were decreased by 43.37% and 61.78% in the 5PPS group, respectively.					
34836688	5	26	theme	controlling	966:976	arg1	reactions					947:955	the chemical reactions	934:955	the chemical reactions	934:955	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	5	26	theme	controlling	966:976	arg1	step					978:981	the controlling step	962:981	the controlling step in the Cr(VI) adsorption process	962:1014	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	8	27	from	%	1433:1433	arg1	group					1447:1451	the 5PPS group	1438:1451	the 5PPS group	1438:1451	Meanwhile, the water-soluble and the leaching Cr contents were decreased by 43.37% and 61.78% in the 5PPS group, respectively.					
34836688	0	28	theme	polyvinyl	42:50	arg1	beads					76:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	7	29	theme	5	1212:1212	arg1	%					1213:1213	%	1213:1213	%	1213:1213	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	10	30	theme	useful	1671:1676	arg1	amendment					1678:1686	a useful amendment	1669:1686	a useful amendment to remediate both the Cr(VI)-contaminated water and soil	1669:1743	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	10	30	theme	useful	1671:1676	arg1	PPS					1656:1658	PPS	1656:1658	PPS	1656:1658	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	7	31	theme	total	1271:1275	arg1	Cr					1277:1278	total Cr	1271:1278	total Cr	1271:1278	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	32	theme	%	1213:1213	arg1	5PPS					1220:1223	5PPS	1220:1223	5PPS	1220:1223	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	32	theme	%	1213:1213	arg1	PPS					1215:1217	the 5% PPS	1208:1217	the 5% PPS (5PPS) treatment	1208:1234	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	6	33	theme	Cr	1156:1157	arg1	removal					1163:1169	Cr(VI) removal	1156:1169	Cr(VI) removal	1156:1169	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	3	34	dep	chromium	528:535	arg1	Cr					538:539	Cr	538:539	Cr(VI)	538:543	Additionally, the PPS was used to recover hexavalent chromium (Cr(VI)) contaminated water and soil to study the remediation impacts and mechanism.					
34836688	7	35	theme	Soil	1172:1175	arg1	results					1188:1194	Soil incubation results	1172:1194	Soil incubation results	1172:1194	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	1	36	theme	difficult	252:260	arg1	soil					247:250	soil difficult	247:260	soil difficult	247:260	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	1	37	from	application	232:242	arg1	soil					247:250	soil difficult	247:260	soil difficult	247:260	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	5	38	theme	pseudo-second-order	848:866	arg1	kinetics					868:875	the pseudo-second-order kinetics	844:875	the pseudo-second-order kinetics	844:875	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	0	39	theme	Cr	11:12	arg1	Removal					0:6	Removal	0:6	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads	0:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	0	40	theme	water	99:103	arg1	treatment					105:113	water treatment	99:113	water treatment	99:113	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	7	41	dep	soil	1321:1324	arg1	VI					1297:1298	VI	1297:1298	VI	1297:1298	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	2	42	theme	polyvinyl	419:427	arg1	PVA					438:440	PVA	438:440	PVA	438:440	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	2	42	theme	polyvinyl	419:427	arg1	alcohol					429:435	polyvinyl alcohol	419:435	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	3	43	dep	Cr	538:539	arg1	VI					541:542	VI	541:542	VI	541:542	Additionally, the PPS was used to recover hexavalent chromium (Cr(VI)) contaminated water and soil to study the remediation impacts and mechanism.					
34836688	4	44	dep	Cr	769:770	arg1	VI					772:773	VI	772:773	VI	772:773	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	0	45	theme	soil	118:121	arg1	remediation					123:133	soil remediation	118:133	soil remediation	118:133	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	3	46	theme	hexavalent	517:526	arg1	chromium					528:535	hexavalent chromium	517:535	hexavalent chromium (Cr(VI)) contaminated water and soil to study the remediation impacts and mechanism	517:619	Additionally, the PPS was used to recover hexavalent chromium (Cr(VI)) contaminated water and soil to study the remediation impacts and mechanism.					
34836688	3	47	used	used	501:504	arg2	PPS					493:495	the PPS	489:495	the PPS	489:495	Additionally, the PPS was used to recover hexavalent chromium (Cr(VI)) contaminated water and soil to study the remediation impacts and mechanism.					
34836688	2	48	theme	alcohol	429:435	arg1	beads					468:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	9	49	theme	Elemental	1468:1476	arg1	speciation					1478:1487	Elemental speciation	1468:1487	Elemental speciation by XPS	1468:1494	Elemental speciation by XPS revealed that Cr(VI) removal from solution and soil proceeded mainly by electrostatic attraction, reduction, and complexation/chelation.					
34836688	4	50	theme	24.24 nm	694:701	arg1	size					686:689	a pore size	679:689	a pore size of 24.24 nm	679:701	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	10	51	theme	-contaminated	1716:1728	arg1	Cr					1710:1711	the Cr(VI)-contaminated water and soil	1706:1743	the Cr(VI)-contaminated water and soil	1706:1743	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	0	52	theme	polyaniline	21:31	arg1	beads					76:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	1	53	theme	strong	277:282	arg1	effect					296:301	the strong aggregation effect	273:301	the strong aggregation effect	273:301	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	2	54	theme	/sodium	442:448	arg1	beads					468:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	4	55	theme	strong	717:722	arg1	83.1 mg/g					745:753	83.1 mg/g	745:753	83.1 mg/g	745:753	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	4	55	theme	strong	717:722	arg1	capacity					735:742	strong adsorption capacity	717:742	strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water	717:783	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	5	56	theme	Cr	990:991	arg1	process					1008:1014	the Cr(VI) adsorption process	986:1014	the Cr(VI) adsorption process	986:1014	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	1	57	theme	aggregation	284:294	arg1	effect					296:301	the strong aggregation effect	273:301	the strong aggregation effect	273:301	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	4	58	with	sphere	667:672	arg1	size					686:689	a pore size	679:689	a pore size of 24.24 nm	679:701	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	5	59	theme	Redlich-Peterson	885:900	arg1	model					911:915	the Redlich-Peterson isotherm model	881:915	the Redlich-Peterson isotherm model	881:915	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	7	60	theme	incubation	1177:1186	arg1	results					1188:1194	Soil incubation results	1172:1194	Soil incubation results	1172:1194	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	10	61	dep	remediate	1691:1699	arg1	both					1701:1704	both	1701:1704	both	1701:1704	The study implied that PPS could be a useful amendment to remediate both the Cr(VI)-contaminated water and soil.					
34836688	5	62	theme	adsorption	997:1006	arg1	process					1008:1014	the Cr(VI) adsorption process	986:1014	the Cr(VI) adsorption process	986:1014	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	5	63	theme	isotherm	902:909	arg1	model					911:915	the Redlich-Peterson isotherm model	881:915	the Redlich-Peterson isotherm model	881:915	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	4	64	theme	pore	681:684	arg1	size					686:689	a pore size	679:689	a pore size of 24.24 nm	679:701	Results showed that the PPS was an irregular sphere with a pore size of 24.24 nm and exhibited strong adsorption capacity (83.1 mg/g) for removing Cr(VI) in water.					
34836688	6	65	theme	13 mg/L	1076:1082	arg1	release					1088:1094	< 13 mg/L TOC release	1074:1094	< 13 mg/L TOC release	1074:1094	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	6	65	theme	13 mg/L	1076:1082	arg1	properties					1062:1071	excellent physicochemical properties	1036:1071	excellent physicochemical properties (< 13 mg/L TOC release)	1036:1095	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	8	66	theme	Cr	1387:1388	arg1	contents					1390:1397	the leaching Cr contents	1374:1397	the leaching Cr contents	1374:1397	Meanwhile, the water-soluble and the leaching Cr contents were decreased by 43.37% and 61.78% in the 5PPS group, respectively.					
34836688	7	67	theme	Cr	1277:1278	arg1	%					1266:1266	24.17%	1261:1266	24.17% of total Cr	1261:1278	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	67	theme	Cr	1277:1278	arg1	Cr					1277:1278	total Cr	1271:1278	total Cr	1271:1278	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	67	theme	Cr	1277:1278	arg1	%					1289:1289	52.47%	1284:1289	52.47% of Cr	1284:1295	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	7	67	theme	Cr	1277:1278	arg1	Cr					1294:1295	Cr	1294:1295	Cr	1294:1295	Soil incubation results showed that the 5% PPS (5PPS) treatment could efficiently remove 24.17% of total Cr and 52.47% of Cr(VI) in the contaminated soil after 30 days.					
34836688	0	68	dep	Cr	11:12	arg1	VI					14:15	VI	14:15	VI	14:15	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	8	69	theme	5PPS	1442:1445	arg1	group					1447:1451	the 5PPS group	1438:1451	the 5PPS group	1438:1451	Meanwhile, the water-soluble and the leaching Cr contents were decreased by 43.37% and 61.78% in the 5PPS group, respectively.					
34836688	5	70	from	step	978:981	arg1	process					1008:1014	the Cr(VI) adsorption process	986:1014	the Cr(VI) adsorption process	986:1014	The Cr(VI) adsorption by PPS could be well described with the pseudo-second-order kinetics and the Redlich-Peterson isotherm model, indicating that the chemical reactions were the controlling step in the Cr(VI) adsorption process.					
34836688	1	71	theme	water	202:206	arg1	industry					208:215	the water industry	198:215	the water industry	198:215	Efficient nano-scale chromium (Cr) remediating agents used in the water industry may find their application in soil difficult because of the strong aggregation effect.					
34836688	2	72	theme	alginate	450:457	arg1	beads					468:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads	419:472	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	6	73	theme	excellent	1036:1044	arg1	release					1088:1094	< 13 mg/L TOC release	1074:1094	< 13 mg/L TOC release	1074:1094	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	6	73	theme	excellent	1036:1044	arg1	properties					1062:1071	excellent physicochemical properties	1036:1071	excellent physicochemical properties (< 13 mg/L TOC release)	1036:1095	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	9	74	from	soil	1543:1546	arg1	removal					1517:1523	removal	1517:1523	removal from solution and soil	1517:1546	Elemental speciation by XPS revealed that Cr(VI) removal from solution and soil proceeded mainly by electrostatic attraction, reduction, and complexation/chelation.					
34836688	2	75	theme	PANI/PVA/SA	338:348	arg1	PPS					361:363	PPS	361:363	PPS	361:363	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	2	75	theme	PANI/PVA/SA	338:348	arg1	composite					350:358	a millimeter-sized PANI/PVA/SA composite	319:358	a millimeter-sized PANI/PVA/SA composite (PPS)	319:364	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	9	76	theme	electrostatic	1568:1580	arg1	attraction					1582:1591	electrostatic attraction	1568:1591	electrostatic attraction	1568:1591	Elemental speciation by XPS revealed that Cr(VI) removal from solution and soil proceeded mainly by electrostatic attraction, reduction, and complexation/chelation.					
34836688	0	77	from	treatment	105:113	arg1	Removal					0:6	Removal	0:6	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads	0:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	0	78	theme	embedded	33:40	arg1	beads					76:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	polyaniline embedded polyvinyl alcohol/sodium alginate beads	21:80	Removal of Cr(VI) by polyaniline embedded polyvinyl alcohol/sodium alginate beads - Extension from water treatment to soil remediation.					
34836688	6	79	dep	removal	1163:1169	arg1	VI					1159:1160	VI	1159:1160	VI	1159:1160	PPS also exhibited excellent physicochemical properties (< 13 mg/L TOC release) and reusability (efficiency of 95.25% after four runs) for Cr(VI) removal.					
34836688	2	80	theme	millimeter-sized	321:336	arg1	PPS					361:363	PPS	361:363	PPS	361:363	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	2	80	theme	millimeter-sized	321:336	arg1	composite					350:358	a millimeter-sized PANI/PVA/SA composite	319:358	a millimeter-sized PANI/PVA/SA composite (PPS)	319:364	In this study, a millimeter-sized PANI/PVA/SA composite (PPS) was synthesized by embedding polyaniline (PANI) into polyvinyl alcohol (PVA)/sodium alginate (SA) gel beads.					
34836688	8	81	theme	leaching	1378:1385	arg1	contents					1390:1397	the leaching Cr contents	1374:1397	the leaching Cr contents	1374:1397	Meanwhile, the water-soluble and the leaching Cr contents were decreased by 43.37% and 61.78% in the 5PPS group, respectively.					
33559732	2	0	theme	cell	340:343	arg1	lines					345:349	cell lines	340:349	cell lines that represents a target for bacterial infection in the host	340:410	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	6	1	from	impact	957:962	arg1	biogenesis					1012:1021	exosome biogenesis	1004:1021	exosome biogenesis	1004:1021	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	6	1	from	impact	957:962	arg1	composition					1033:1043	cargo composition	1027:1043	cargo composition	1027:1043	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	7	2	from	mice	1251:1254	arg1	EVs					1234:1236	EVs	1234:1236	EVs from control mice	1234:1254	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	8	3	dep	in	1344:1345	arg1	vitro					1347:1351	vitro	1347:1351	vitro	1347:1351	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	2	4	from	impact	283:288	arg1	lines					345:349	cell lines	340:349	cell lines that represents a target for bacterial infection in the host	340:410	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	6	5	theme	BALB/c	1048:1053	arg1	mice					1055:1058	BALB/c mice	1048:1058	BALB/c mice	1048:1058	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	6	6	theme	cargo	1027:1031	arg1	composition					1033:1043	cargo composition	1027:1043	cargo composition	1027:1043	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	3	7	theme	cell	534:537	arg1	death					539:543	cell death	534:543	cell death	534:543	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	3	8	theme	cell	493:496	arg1	lines					498:502	BV-2 cell lines	488:502	BV-2 cell lines	488:502	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	2	9	dep	in	274:275	arg1	vitro					277:281	vitro	277:281	vitro	277:281	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	7	10	theme	receptor	1202:1209	arg1	levels					1213:1218	significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels	1124:1218	significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels	1124:1218	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	3	11	theme	modest	516:521	arg1	changes					523:529	only modest changes	511:529	only modest changes	511:529	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	8	12	theme	in	1344:1345	arg1	models					1365:1370	these in vitro and in vivo models	1338:1370	these in vitro and in vivo models of bacterial infection	1338:1393	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	5	13	theme	concentrations	895:908	arg1	administration					858:871	administration	858:871	administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL	858:933	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	7	14	theme	control	1243:1249	arg1	mice					1251:1254	control mice	1243:1254	control mice	1243:1254	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	1	15	theme	Extracellular	88:100	arg1	EVs					112:114	EVs	112:114	EVs	112:114	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	1	15	theme	Extracellular	88:100	arg1	vesicles					102:109	Extracellular vesicles	88:109	Extracellular vesicles (EVs)	88:115	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	4	16	theme	lipopolysaccharide	639:656	arg1	concentration					622:634	the highest concentration	610:634	the highest concentration of lipopolysaccharide	610:656	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	5	17	theme	less	825:828	arg1	factor					845:850	significantly less tumor necrosis factor	811:850	significantly less tumor necrosis factor	811:850	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	4	18	theme	membrane	754:761	arg1	protein					763:769	lysosomal-associated membrane protein 1	733:771	lysosomal-associated membrane protein 1	733:771	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	5	19	theme	0.1 µg/mL	913:921	arg1	concentrations					895:908	lipopolysaccharide concentrations	876:908	lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL	876:933	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	0	20	theme	Lipopolysaccharide	0:17	arg1	Administration					19:32	Lipopolysaccharide Administration	0:32	Lipopolysaccharide Administration	0:32	Lipopolysaccharide Administration Alters Extracellular Vesicles in Cell Lines and Mice.					
33559732	7	21	theme	toll-like	1192:1200	arg1	receptor					1202:1209	toll-like receptor 4	1192:1211	toll-like receptor 4	1192:1211	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	3	22	theme	EV	550:551	arg1	numbers					553:559	EV numbers	550:559	EV numbers	550:559	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	0	23	theme	Extracellular	41:53	arg1	Vesicles					55:62	Extracellular Vesicles	41:62	Extracellular Vesicles	41:62	Lipopolysaccharide Administration Alters Extracellular Vesicles in Cell Lines and Mice.					
33559732	5	24	theme	tumor	830:834	arg1	factor					845:850	significantly less tumor necrosis factor	811:850	significantly less tumor necrosis factor	811:850	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	7	25	theme	protein	1178:1184	arg1	levels					1213:1218	significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels	1124:1218	significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels	1124:1218	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	8	26	theme	EV	1298:1299	arg1	numbers					1301:1307	EV numbers	1298:1307	EV numbers	1298:1307	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	2	27	theme	in	274:275	arg1	impact					283:288	the in vitro impact	270:288	the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host	270:410	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	3	28	theme	varying	453:459	arg1	concentrations					461:474	varying concentrations	453:474	varying concentrations	453:474	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	4	29	theme	lysosomal-associated	733:752	arg1	protein					763:769	lysosomal-associated membrane protein 1	733:771	lysosomal-associated membrane protein 1	733:771	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	7	30	theme	lysosomal-associated	1148:1167	arg1	protein					1178:1184	lysosomal-associated membrane protein 1	1148:1186	lysosomal-associated membrane protein 1	1148:1186	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	6	31	from	biogenesis	1012:1021	arg1	mice					1055:1058	BALB/c mice	1048:1058	BALB/c mice	1048:1058	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	1	32	dep	cell	144:147	arg1	biology					163:169	biology	163:169	biology	163:169	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	5	33	theme	necrosis	836:843	arg1	factor					845:850	significantly less tumor necrosis factor	811:850	significantly less tumor necrosis factor	811:850	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	5	34	theme	BV-2	791:794	arg1	cells					796:800	BV-2 cells	791:800	BV-2 cells	791:800	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	3	35	theme	BV-2	488:491	arg1	lines					498:502	BV-2 cell lines	488:502	BV-2 cell lines	488:502	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	6	36	from	composition	1033:1043	arg1	mice					1055:1058	BALB/c mice	1048:1058	BALB/c mice	1048:1058	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	1	37	contain	have	175:178	arg1	EVs					112:114	EVs	112:114	EVs	112:114	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	1	37	contain	have	175:178	arg2	potential					184:192	the potential to act as biomarkers for novel diagnostic tools	180:240	the potential to act as biomarkers for novel diagnostic tools	180:240	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	1	37	contain	have	175:178	arg1	vesicles					102:109	Extracellular vesicles	88:109	Extracellular vesicles (EVs)	88:115	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	8	38	theme	infection	1385:1393	arg1	models					1365:1370	these in vitro and in vivo models	1338:1370	these in vitro and in vivo models of bacterial infection	1338:1393	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	3	39	theme	lipopolysaccharide	431:448	arg1	Administration					413:426	Administration	413:426	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines	413:502	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	6	40	theme	exosome	1004:1010	arg1	biogenesis					1012:1021	exosome biogenesis	1004:1021	exosome biogenesis	1004:1021	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	7	41	theme	membrane	1169:1176	arg1	protein					1178:1184	lysosomal-associated membrane protein 1	1148:1186	lysosomal-associated membrane protein 1	1148:1186	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	4	42	with	treatment	595:603	arg1	concentration					622:634	the highest concentration	610:634	the highest concentration of lipopolysaccharide	610:656	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	2	43	theme	bacterial	380:388	arg1	infection					390:398	bacterial infection	380:398	bacterial infection in the host	380:410	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	5	44	theme	1 µg/mL	927:933	arg1	concentrations					895:908	lipopolysaccharide concentrations	876:908	lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL	876:933	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	4	45	attach	derived	663:669	arg1	cells					681:685	A549 cells	676:685	A549 cells	676:685	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	4	45	attach	derived	663:669	arg2	EVs					659:661	EVs	659:661	EVs derived from A549 cells	659:685	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	7	46	theme	lipopolysaccharide-treated	1085:1110	arg1	mice					1112:1115	lipopolysaccharide-treated mice	1085:1115	lipopolysaccharide-treated mice	1085:1115	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	2	47	theme	administration	322:335	arg1	impact					283:288	the in vitro impact	270:288	the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host	270:410	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	0	48	theme	Cell	67:70	arg1	Lines					72:76	Cell Lines	67:76	Cell Lines	67:76	Lipopolysaccharide Administration Alters Extracellular Vesicles in Cell Lines and Mice.					
33559732	6	49	theme	administration	986:999	arg1	impact					957:962	the impact	953:962	the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice	953:1058	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	7	50	theme	increased	1138:1146	arg1	levels					1213:1218	significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels	1124:1218	significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels	1124:1218	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	7	51	theme	Serum-isolated	1061:1074	arg1	EVs					1076:1078	Serum-isolated EVs	1061:1078	Serum-isolated EVs from lipopolysaccharide-treated mice	1061:1115	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	2	52	theme	lipopolysaccharide	303:320	arg1	administration					322:335	bacterial lipopolysaccharide administration	293:335	bacterial lipopolysaccharide administration	293:335	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	6	53	theme	lipopolysaccharide	967:984	arg1	administration					986:999	lipopolysaccharide administration	967:999	lipopolysaccharide administration	967:999	We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice.					
33559732	4	54	theme	less	710:713	arg1	interleukin-6					715:727	significantly less interleukin-6	696:727	significantly less interleukin-6	696:727	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	3	55	from	concentrations	461:474	arg1	Administration					413:426	Administration	413:426	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines	413:502	Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed.					
33559732	1	56	theme	fundamental	124:134	arg1	role					136:139	a fundamental role	122:139	a fundamental role	122:139	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	7	57	from	mice	1112:1115	arg1	EVs					1076:1078	Serum-isolated EVs	1061:1078	Serum-isolated EVs from lipopolysaccharide-treated mice	1061:1115	Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice.					
33559732	5	58	attach	derived	778:784	arg2	EVs					774:776	EVs	774:776	EVs derived from BV-2 cells	774:800	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	5	58	attach	derived	778:784	arg1	cells					796:800	BV-2 cells	791:800	BV-2 cells	791:800	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	2	59	from	infection	390:398	arg1	host					407:410	the host	403:410	the host	403:410	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	8	60	theme	bacterial	1375:1383	arg1	infection					1385:1393	bacterial infection	1375:1393	bacterial infection	1375:1393	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	2	61	theme	bacterial	293:301	arg1	lipopolysaccharide					303:320	bacterial lipopolysaccharide	293:320	bacterial lipopolysaccharide administration	293:335	In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host.					
33559732	5	62	theme	lipopolysaccharide	876:893	arg1	concentrations					895:908	lipopolysaccharide concentrations	876:908	lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL	876:933	EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL.					
33559732	8	63	theme	in	1357:1358	arg1	models					1365:1370	these in vitro and in vivo models	1338:1370	these in vitro and in vivo models of bacterial infection	1338:1393	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	4	64	theme	highest	614:620	arg1	concentration					622:634	the highest concentration	610:634	the highest concentration of lipopolysaccharide	610:656	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	4	65	theme	A549	676:679	arg1	cells					681:685	A549 cells	676:685	A549 cells	676:685	After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1.					
33559732	1	66	theme	novel	219:223	arg1	tools					236:240	novel diagnostic tools	219:240	novel diagnostic tools	219:240	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33559732	8	67	dep	in	1357:1358	arg1	vivo					1360:1363	vivo	1360:1363	vivo	1360:1363	In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.					
33559732	1	68	theme	diagnostic	225:234	arg1	tools					236:240	novel diagnostic tools	219:240	novel diagnostic tools	219:240	Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools.					
33233233	8	0	theme	E	1070:1070	arg1	cheeses					1072:1078	Ep and E cheeses	1063:1078	Ep and E cheeses (0.88 and 0.51 g/100 g, respectively)	1063:1116	GOS were only detected in Ep and E cheeses (0.88 and 0.51 g/100 g, respectively).					
33233233	7	1	dep	cheese	1009:1014	arg1	ripening					1027:1034	ripening	1027:1034	ripening	1027:1034	Carbohydrate fermentation profiles differed among treatments during cheese making and ripening.					
33233233	7	1	dep	cheese	1009:1014	arg1	making					1016:1021	making	1016:1021	making	1016:1021	Carbohydrate fermentation profiles differed among treatments during cheese making and ripening.					
33233233	2	2	theme	making	289:294	arg1	cheese					282:287	soft cheese making	277:294	soft cheese making	277:294	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	11	3	dep	contents	1621:1628	arg1	the					1604:1606	the	1604:1606	the	1604:1606	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	2	4	theme	soft	277:280	arg1	cheese					282:287	soft cheese making	277:294	soft cheese making	277:294	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	1	5	theme	prebiotic	186:194	arg1	role					196:199	recognized prebiotic role	175:199	recognized prebiotic role	175:199	Galactooligosaccharides (GOS) are non-digestible oligosaccharides with recognized prebiotic role.					
33233233	4	6	theme	enzyme	644:649	arg1	addition					651:658	enzyme addition	644:658	enzyme addition	644:658	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	13	7	theme	technological	1834:1846	arg1	satisfying					1874:1883	satisfying	1874:1883	satisfying	1874:1883	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	13	7	theme	technological	1834:1846	arg1	approach					1848:1855	the proposed technological approach	1821:1855	the proposed technological approach	1821:1855	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	5	8	theme	organic	817:823	arg1	profiles					830:837	organic acid profiles	817:837	organic acid profiles	817:837	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	4	9	dep	addition	583:590	arg1	cheese					629:634	cheese	629:634	the starter addition (Ep and E treatments, respectively); cheese	571:634	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	4	9	dep	addition	583:590	arg1	treatments					602:611	E treatments	600:611	E treatments	600:611	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	4	9	dep	addition	583:590	arg1	Ep					593:594	Ep	593:594	Ep	593:594	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	5	10	theme	fraction	785:792	arg1	view					764:767	view	764:767	view of carbohydrate fraction	764:792	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	5	11	theme	view	764:767	arg1	point					755:759	the point	751:759	the point of view of carbohydrate fraction	751:792	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	4	12	theme	C	675:675	arg1	treatment					677:685	C treatment	675:685	C treatment	675:685	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	10	13	theme	carbohydrate	1506:1517	arg1	profile					1519:1525	the carbohydrate profile	1502:1525	the carbohydrate profile	1502:1525	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	13	14	theme	desired	1805:1811	arg1	effect					1813:1818	the desired effect	1801:1818	the desired effect	1801:1818	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	7	15	theme	fermentation	954:965	arg1	profiles					967:974	Carbohydrate fermentation profiles	941:974	Carbohydrate fermentation profiles	941:974	Carbohydrate fermentation profiles differed among treatments during cheese making and ripening.					
33233233	5	16	theme	fresh	712:716	arg1	cheeses					738:744	fresh and soft commercial cheeses	712:744	fresh and soft commercial cheeses	712:744	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	1	17	theme	non-digestible	138:151	arg1	Galactooligosaccharides					104:126	Galactooligosaccharides	104:126	Galactooligosaccharides (GOS)	104:132	Galactooligosaccharides (GOS) are non-digestible oligosaccharides with recognized prebiotic role.					
33233233	1	17	theme	non-digestible	138:151	arg1	oligosaccharides					153:168	non-digestible oligosaccharides	138:168	non-digestible oligosaccharides with recognized prebiotic role	138:199	Galactooligosaccharides (GOS) are non-digestible oligosaccharides with recognized prebiotic role.					
33233233	11	18	located	detected	1541:1548	arg2	GOS					1528:1530	GOS	1528:1530	GOS	1528:1530	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	11	18	located	detected	1541:1548	arg1	any					1553:1555	any	1553:1555	any	1553:1555	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	5	19	theme	soft	722:725	arg1	cheeses					738:744	fresh and soft commercial cheeses	712:744	fresh and soft commercial cheeses	712:744	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	2	20	from	impact	315:320	arg1	metabolism					339:348	metabolism	339:348	metabolism	339:348	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	20	from	impact	315:320	arg1	characteristics					435:449	physicochemical and microbiological characteristics	399:449	physicochemical and microbiological characteristics of the product	399:464	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	20	from	impact	315:320	arg1	carbohydrates					325:337	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	20	from	impact	315:320	arg1	proteolysis					351:361	proteolysis	351:361	proteolysis	351:361	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	20	from	impact	315:320	arg1	production					387:396	volatile compounds production	368:396	volatile compounds production	368:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	6	21	theme	target	923:928	arg1	~5.2					934:937	~5.2	934:937	~5.2	934:937	The inclusion of the enzyme in soft cheese making produced a delay in reaching the target pH (~5.2).					
33233233	6	21	theme	target	923:928	arg1	pH					930:931	the target pH	919:931	the target pH (~5.2)	919:938	The inclusion of the enzyme in soft cheese making produced a delay in reaching the target pH (~5.2).					
33233233	0	22	from	Effect	0:5	arg1	production					58:67	the GOS production	50:67	the GOS production	50:67	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	10	23	theme	volatile	1343:1350	arg1	compounds					1352:1360	volatile compounds	1343:1360	volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids	1343:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	3	24	dep	milk	498:501	arg1	milk					510:513	fluid milk	504:513	fluid milk	504:513	The enzyme was added to cheese milk (fluid milk plus whey powder) before (40 min.)					
33233233	3	24	dep	milk	498:501	arg1	powder					525:530	whey powder	520:530	whey powder	520:530	The enzyme was added to cheese milk (fluid milk plus whey powder) before (40 min.)					
33233233	2	25	theme	physicochemical	399:413	arg1	characteristics					435:449	physicochemical and microbiological characteristics	399:449	physicochemical and microbiological characteristics of the product	399:464	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	25	theme	physicochemical	399:413	arg1	carbohydrates					325:337	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	0	26	theme	cheese	96:101	arg1	manufacture					76:86	manufacture	76:86	manufacture of soft cheese	76:101	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	0	27	theme	β-galactosidase	31:45	arg1	incorporation					14:26	the incorporation	10:26	the incorporation of β-galactosidase in the GOS production	10:67	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	14	28	theme	prebiotic	1912:1920	arg1	fiber					1922:1926	prebiotic fiber	1912:1926	prebiotic fiber	1912:1926	Cheeses with prebiotic fiber are not still widespread in the market.					
33233233	2	29	dep	carbohydrates	325:337	arg1	metabolism					339:348	metabolism	339:348	metabolism	339:348	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	29	dep	carbohydrates	325:337	arg1	production					387:396	volatile compounds production	368:396	volatile compounds production	368:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	29	dep	carbohydrates	325:337	arg1	proteolysis					351:361	proteolysis	351:361	proteolysis	351:361	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	29	dep	carbohydrates	325:337	arg1	carbohydrates					325:337	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	30	theme	product	458:464	arg1	characteristics					435:449	physicochemical and microbiological characteristics	399:449	physicochemical and microbiological characteristics of the product	399:464	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	30	theme	product	458:464	arg1	carbohydrates					325:337	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	10	31	theme	ketones	1411:1417	arg1	families					1388:1395	the chemical families	1375:1395	the chemical families of aldehydes, ketones, alcohols, esters and acids	1375:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	9	32	theme	physicochemical	1229:1243	arg1	profiles					1289:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	10	33	theme	aldehydes	1400:1408	arg1	families					1388:1395	the chemical families	1375:1395	the chemical families of aldehydes, ketones, alcohols, esters and acids	1375:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	2	34	theme	microbiological	419:433	arg1	characteristics					435:449	physicochemical and microbiological characteristics	399:449	physicochemical and microbiological characteristics of the product	399:464	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	34	theme	microbiological	419:433	arg1	carbohydrates					325:337	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	9	35	theme	microbial	1249:1257	arg1	composition					1259:1269	microbial composition	1249:1269	microbial composition	1249:1269	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	12	36	theme	obtaining	1712:1720	arg1	cheeses					1722:1728	obtaining cheeses	1712:1728	obtaining cheeses with GOS	1712:1737	The results obtained demonstrate the feasibility of obtaining cheeses with GOS.					
33233233	10	37	theme	esters	1430:1435	arg1	families					1388:1395	the chemical families	1375:1395	the chemical families of aldehydes, ketones, alcohols, esters and acids	1375:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	0	38	theme	GOS	54:56	arg1	production					58:67	the GOS production	50:67	the GOS production	50:67	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	10	39	theme	alcohols	1420:1427	arg1	families					1388:1395	the chemical families	1375:1395	the chemical families of aldehydes, ketones, alcohols, esters and acids	1375:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	3	40	theme	cheese	491:496	arg1	milk					498:501	cheese milk	491:501	cheese milk (fluid milk plus whey powder)	491:531	The enzyme was added to cheese milk (fluid milk plus whey powder) before (40 min.)					
33233233	9	41	theme	acids	1283:1287	arg1	profiles					1289:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	14	42	from	widespread	1942:1951	arg1	market					1960:1965	the market	1956:1965	the market	1956:1965	Cheeses with prebiotic fiber are not still widespread in the market.					
33233233	3	43	theme	whey	520:523	arg1	powder					525:530	whey powder	520:530	whey powder	520:530	The enzyme was added to cheese milk (fluid milk plus whey powder) before (40 min.)					
33233233	12	44	with	cheeses	1722:1728	arg1	GOS					1735:1737	GOS	1735:1737	GOS	1735:1737	The results obtained demonstrate the feasibility of obtaining cheeses with GOS.					
33233233	6	45	theme	enzyme	861:866	arg1	inclusion					844:852	The inclusion	840:852	The inclusion of the enzyme in soft cheese making	840:888	The inclusion of the enzyme in soft cheese making produced a delay in reaching the target pH (~5.2).					
33233233	10	46	theme	acids	1441:1445	arg1	families					1388:1395	the chemical families	1375:1395	the chemical families of aldehydes, ketones, alcohols, esters and acids	1375:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	13	47	theme	proposed	1825:1832	arg1	satisfying					1874:1883	satisfying	1874:1883	satisfying	1874:1883	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	13	47	theme	proposed	1825:1832	arg1	approach					1848:1855	the proposed technological approach	1821:1855	the proposed technological approach	1821:1855	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	4	48	theme	starter	575:581	arg1	addition					583:590	the starter addition	571:590	the starter addition (Ep and E treatments, respectively); cheese	571:634	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	11	49	theme	commercial	1564:1573	arg1	cheeses					1575:1581	the commercial cheeses	1560:1581	the commercial cheeses	1560:1581	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	1	50	theme	recognized	175:184	arg1	role					196:199	recognized prebiotic role	175:199	recognized prebiotic role	175:199	Galactooligosaccharides (GOS) are non-digestible oligosaccharides with recognized prebiotic role.					
33233233	9	51	theme	Lactose	1119:1125	arg1	content					1127:1133	Lactose content	1119:1133	Lactose content	1119:1133	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	5	52	theme	carbohydrate	772:783	arg1	fraction					785:792	carbohydrate fraction	772:792	carbohydrate fraction	772:792	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	14	53	with	Cheeses	1899:1905	arg1	fiber					1922:1926	prebiotic fiber	1912:1926	prebiotic fiber	1912:1926	Cheeses with prebiotic fiber are not still widespread in the market.					
33233233	6	54	theme	making	883:888	arg1	cheese					876:881	soft cheese making	871:888	soft cheese making	871:888	The inclusion of the enzyme in soft cheese making produced a delay in reaching the target pH (~5.2).					
33233233	0	55	from	production	58:67	arg1	Effect					0:5	Effect	0:5	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.	0:102	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	9	56	theme	glucose	1150:1156	arg1	levels					1172:1177	glucose and galactose levels	1150:1177	glucose and galactose levels	1150:1177	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	6	57	theme	soft	871:874	arg1	cheese					876:881	soft cheese making	871:888	soft cheese making	871:888	The inclusion of the enzyme in soft cheese making produced a delay in reaching the target pH (~5.2).					
33233233	7	58	theme	Carbohydrate	941:952	arg1	profiles					967:974	Carbohydrate fermentation profiles	941:974	Carbohydrate fermentation profiles	941:974	Carbohydrate fermentation profiles differed among treatments during cheese making and ripening.					
33233233	9	59	theme	galactose	1162:1170	arg1	levels					1172:1177	glucose and galactose levels	1150:1177	glucose and galactose levels	1150:1177	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	2	60	theme	K.	260:261	arg1	lactis					263:268	K. lactis	260:268	K. lactis	260:268	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	5	61	theme	acid	825:828	arg1	profiles					830:837	organic acid profiles	817:837	organic acid profiles	817:837	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	0	62	from	incorporation	14:26	arg1	production					58:67	the GOS production	50:67	the GOS production	50:67	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	11	63	theme	organic	1634:1640	arg1	acids					1642:1646	organic acids	1634:1646	organic acids	1634:1646	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	1	64	with	oligosaccharides	153:168	arg1	role					196:199	recognized prebiotic role	175:199	recognized prebiotic role	175:199	Galactooligosaccharides (GOS) are non-digestible oligosaccharides with recognized prebiotic role.					
33233233	10	65	theme	compounds	1352:1360	arg1	Bioformation					1327:1338	Bioformation	1327:1338	Bioformation	1327:1338	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	8	66	located	detected	1051:1058	arg2	GOS					1037:1039	GOS	1037:1039	GOS	1037:1039	GOS were only detected in Ep and E cheeses (0.88 and 0.51 g/100 g, respectively).					
33233233	8	66	located	detected	1051:1058	arg1	cheeses					1072:1078	Ep and E cheeses	1063:1078	Ep and E cheeses (0.88 and 0.51 g/100 g, respectively)	1063:1116	GOS were only detected in Ep and E cheeses (0.88 and 0.51 g/100 g, respectively).					
33233233	0	67	theme	soft	91:94	arg1	cheese					96:101	soft cheese	91:101	soft cheese	91:101	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	4	68	dep	made	669:672	arg1	treatment					677:685	C treatment	675:685	C treatment	675:685	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	8	69	dep	cheeses	1072:1078	arg1	0.51 g/100 g					1090:1101	0.51 g/100 g	1090:1101	0.51 g/100 g	1090:1101	GOS were only detected in Ep and E cheeses (0.88 and 0.51 g/100 g, respectively).					
33233233	8	69	dep	cheeses	1072:1078	arg1	0.88					1081:1084	0.88	1081:1084	0.88	1081:1084	GOS were only detected in Ep and E cheeses (0.88 and 0.51 g/100 g, respectively).					
33233233	13	70	theme	GOS	1753:1755	arg1	values					1757:1762	the GOS values	1749:1762	the GOS values achieved	1749:1771	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	13	70	theme	GOS	1753:1755	arg1	adequate					1781:1788	adequate	1781:1788	adequate	1781:1788	Although the GOS values achieved are not adequate enough for the desired effect, the proposed technological approach turned out to be satisfying and original.					
33233233	11	71	from	variations	1590:1599	arg1	acids					1642:1646	organic acids	1634:1646	organic acids	1634:1646	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	11	71	from	variations	1590:1599	arg1	contents					1621:1628	carbohydrate contents	1608:1628	carbohydrate contents	1608:1628	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	10	72	theme	chemical	1379:1386	arg1	families					1388:1395	the chemical families	1375:1395	the chemical families of aldehydes, ketones, alcohols, esters and acids	1375:1445	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	9	73	from	differences	1214:1224	arg1	profiles					1289:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	2	74	theme	compounds	377:385	arg1	production					387:396	volatile compounds production	368:396	volatile compounds production	368:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	2	74	theme	compounds	377:385	arg1	carbohydrates					325:337	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	carbohydrates metabolism, proteolysis, and volatile compounds production	325:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	0	75	theme	incorporation	14:26	arg1	Effect					0:5	Effect	0:5	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.	0:102	Effect of the incorporation of β-galactosidase in the GOS production during manufacture of soft cheese.					
33233233	11	76	theme	great	1584:1588	arg1	variations					1590:1599	great variations	1584:1599	great variations in the carbohydrate contents and organic acids	1584:1646	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	2	77	theme	volatile	368:375	arg1	compounds					377:385	volatile compounds	368:385	volatile compounds production	368:396	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	5	78	theme	commercial	727:736	arg1	cheeses					738:744	fresh and soft commercial cheeses	712:744	fresh and soft commercial cheeses	712:744	Also, we characterized fresh and soft commercial cheeses from the point of view of carbohydrate fraction, highlighting GOS, and organic acid profiles.					
33233233	2	79	from	lactis	263:268	arg1	β-galactosidase					239:253	a β-galactosidase	237:253	a β-galactosidase from K. lactis	237:268	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	9	80	dep	C.	1208:1209	arg1	observed					1317:1324	observed	1317:1324	were observed	1312:1324	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	9	81	theme	composition	1259:1269	arg1	profiles					1289:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	physicochemical and microbial composition and organic acids profiles	1229:1296	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	14	82	from	market	1960:1965	arg1	widespread					1942:1951	widespread	1942:1951	widespread	1942:1951	Cheeses with prebiotic fiber are not still widespread in the market.					
33233233	10	83	from	modification	1486:1497	arg1	profile					1519:1525	the carbohydrate profile	1502:1525	the carbohydrate profile	1502:1525	Bioformation of volatile compounds belonging to the chemical families of aldehydes, ketones, alcohols, esters and acids, was not substantially affected by the modification in the carbohydrate profile.					
33233233	11	84	theme	carbohydrate	1608:1619	arg1	contents					1621:1628	carbohydrate contents	1608:1628	carbohydrate contents	1608:1628	GOS were not detected in any of the commercial cheeses; great variations in the carbohydrate contents and organic acids were found.					
33233233	3	85	theme	fluid	504:508	arg1	milk					510:513	fluid milk	504:513	fluid milk	504:513	The enzyme was added to cheese milk (fluid milk plus whey powder) before (40 min.)					
33233233	9	86	theme	organic	1275:1281	arg1	acids					1283:1287	organic acids	1275:1287	organic acids	1275:1287	Lactose content was lower, and glucose and galactose levels were higher in E and Ep than C. No differences in physicochemical and microbial composition and organic acids profiles among samples were observed.					
33233233	12	87	theme	cheeses	1722:1728	arg1	feasibility					1697:1707	the feasibility	1693:1707	the feasibility of obtaining cheeses with GOS	1693:1737	The results obtained demonstrate the feasibility of obtaining cheeses with GOS.					
33233233	2	88	theme	present	206:212	arg1	study					214:218	The present study	202:218	The present study	202:218	The present study aims to evaluate a β-galactosidase from K. lactis during soft cheese making and to analyse the impact on carbohydrates metabolism, proteolysis, and volatile compounds production, physicochemical and microbiological characteristics of the product.					
33233233	8	89	theme	Ep	1063:1064	arg1	cheeses					1072:1078	Ep and E cheeses	1063:1078	Ep and E cheeses (0.88 and 0.51 g/100 g, respectively)	1063:1116	GOS were only detected in Ep and E cheeses (0.88 and 0.51 g/100 g, respectively).					
33233233	3	90	dep	added	482:486	arg1	40 min					541:546	40 min	541:546	40 min	541:546	The enzyme was added to cheese milk (fluid milk plus whey powder) before (40 min.)					
33233233	4	91	theme	E	600:600	arg1	treatments					602:611	E treatments	600:611	E treatments	600:611	or simultaneously of the starter addition (Ep and E treatments, respectively); cheese without enzyme addition was also made (C treatment).					
33233233	6	92	from	inclusion	844:852	arg1	cheese					876:881	soft cheese making	871:888	soft cheese making	871:888	The inclusion of the enzyme in soft cheese making produced a delay in reaching the target pH (~5.2).					
32422267	0	0	theme	compounds	84:92	arg1	oxidation					62:70	oxidation	62:70	oxidation	62:70	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	0	0	theme	compounds	84:92	arg1	optimization					45:56	optimization	45:56	optimization	45:56	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	0	0	theme	compounds	84:92	arg1	Preparation					32:42	Preparation	32:42	Preparation	32:42	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	5	1	theme	phenol	688:693	arg1	removal					677:683	the removal	673:683	the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution	673:774	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	0	2	theme	phenolic	75:82	arg1	compounds					84:92	phenolic compounds	75:92	phenolic compounds	75:92	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	4	3	theme	higher	554:559	arg1	stability					561:569	the higher stability	550:569	the higher stability	550:569	IPPO-Au exhibited the higher stability and reusability than that of IPPO.					
32422267	5	4	theme	immobilized	639:649	arg1	PPO					651:653	immobilized PPO	639:653	immobilized PPO	639:653	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	9	5	theme	substrate	1369:1377	arg1	structure					1388:1396	substrate chemical structure	1369:1396	substrate chemical structure	1369:1396	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	10	6	dep	suggested	1449:1457	arg1	played					1486:1491	played	1486:1491	suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO	1449:1565	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	5	7	theme	PPO	651:653	arg1	performance					624:634	The bio-catalytic performance	606:634	The bio-catalytic performance of immobilized PPO	606:653	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	2	8	theme	immobilization	316:329	arg1	conditions					331:340	the optimal immobilization conditions	304:340	the optimal immobilization conditions for achieving the maximum enzyme activity	304:382	Taguchi method was applied to determine the optimal immobilization conditions for achieving the maximum enzyme activity.					
32422267	5	9	attach	removal	677:683	arg2	4-CP					717:720	4-CP	717:720	4-CP	717:720	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	5	9	attach	removal	677:683	arg2	phenol					688:693	phenol	688:693	phenol (PH)	688:698	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	5	9	attach	removal	677:683	arg2	2,4-DCP					747:753	2,4-DCP	747:753	2,4-DCP	747:753	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	5	9	attach	removal	677:683	arg2	PH					696:697	PH	696:697	PH	696:697	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	5	9	attach	removal	677:683	arg2	2,4-dichlorophenol					727:744	2,4-dichlorophenol	727:744	2,4-dichlorophenol (2,4-DCP)	727:754	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	5	9	attach	removal	677:683	arg3	solution					767:774	aqueous solution	759:774	aqueous solution	759:774	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	5	9	attach	removal	677:683	arg2	4-chlorophenol					701:714	4-chlorophenol	701:714	4-chlorophenol (4-CP)	701:721	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	9	10	theme	chemical	1379:1386	arg1	structure					1388:1396	substrate chemical structure	1369:1396	substrate chemical structure	1369:1396	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	10	11	theme	PPO	1563:1565	arg1	activity					1525:1532	enzyme activity	1518:1532	enzyme activity	1518:1532	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	10	11	theme	PPO	1563:1565	arg1	stability					1538:1546	stability	1538:1546	stability	1538:1546	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	7	12	theme	optimal	1064:1070	arg1	temperature					1072:1082	the optimal temperature	1060:1082	the optimal temperature	1060:1082	The results showed that, for both immobilized PPO, the optimal pH was 7, 6 and 5 for PH, 4-CP and 2,4-DCP, respectively, and the optimal temperature was 30 °C for all substrate.					
32422267	2	13	theme	optimal	308:314	arg1	conditions					331:340	the optimal immobilization conditions	304:340	the optimal immobilization conditions for achieving the maximum enzyme activity	304:382	Taguchi method was applied to determine the optimal immobilization conditions for achieving the maximum enzyme activity.					
32422267	9	14	theme	structure	1388:1396	arg1	effect					1359:1364	the effect	1355:1364	the effect of substrate chemical structure on the IPPO-Au properties	1355:1422	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	9	15	theme	improved	1271:1278	arg1	efficiency					1290:1299	the improved catalytic efficiency	1267:1299	the improved catalytic efficiency in comparison with PH for both immobilized PPO	1267:1346	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	9	16	theme	IPPO-Au	1405:1411	arg1	properties					1413:1422	the IPPO-Au properties	1401:1422	the IPPO-Au properties	1401:1422	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	3	17	theme	highest	458:464	arg1	activity					473:480	the highest enzyme activity	454:480	the highest enzyme activity	454:480	PPO immobilized on CTS/MMT (IPPO) and CTS-AuNPs/MMT (IPPO-Au) showed the highest enzyme activity at 15.61 × 103 and 29.01 × 103 U/g, respectively.					
32422267	5	18	theme	aqueous	759:765	arg1	solution					767:774	aqueous solution	759:774	aqueous solution	759:774	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	9	19	theme	catalytic	1280:1288	arg1	efficiency					1290:1299	the improved catalytic efficiency	1267:1299	the improved catalytic efficiency in comparison with PH for both immobilized PPO	1267:1346	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	6	20	dep	compounds	888:896	arg1	removal					898:904	removal	898:904	the phenolic compounds removal	875:904	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	3	21	theme	enzyme	466:471	arg1	activity					473:480	the highest enzyme activity	454:480	the highest enzyme activity	454:480	PPO immobilized on CTS/MMT (IPPO) and CTS-AuNPs/MMT (IPPO-Au) showed the highest enzyme activity at 15.61 × 103 and 29.01 × 103 U/g, respectively.					
32422267	0	22	theme	polyphenol	12:21	arg1	oxidase					23:29	Immobilized polyphenol oxidase	0:29	Immobilized polyphenol oxidase	0:29	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	10	23	theme	important	1496:1504	arg1	role					1506:1509	an important role	1493:1509	an important role	1493:1509	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	5	24	theme	bio-catalytic	610:622	arg1	performance					624:634	The bio-catalytic performance	606:634	The bio-catalytic performance of immobilized PPO	606:653	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	9	25	from	effect	1359:1364	arg1	properties					1413:1422	the IPPO-Au properties	1401:1422	the IPPO-Au properties	1401:1422	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	1	26	theme	chitosan-gold	178:190	arg1	CTS-AuNPs/MMT					223:235	CTS-AuNPs/MMT	223:235	CTS-AuNPs/MMT	223:235	Polyphenol oxidase (PPO) was immobilized on chitosan/montmorillonite (CTS/MMT) and chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT) composites, respectively.					
32422267	1	26	theme	chitosan-gold	178:190	arg1	nanoparticles/montmorillonite					192:220	chitosan-gold nanoparticles/montmorillonite	178:220	chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT)	178:236	Polyphenol oxidase (PPO) was immobilized on chitosan/montmorillonite (CTS/MMT) and chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT) composites, respectively.					
32422267	0	27	theme	Immobilized	0:10	arg1	oxidase					23:29	Immobilized polyphenol oxidase	0:29	Immobilized polyphenol oxidase	0:29	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	5	28	from	removal	677:683	arg1	solution					767:774	aqueous solution	759:774	aqueous solution	759:774	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	6	29	theme	pH	792:793	arg1	effects					781:787	The effects	777:787	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal	777:904	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	1	30	theme	Polyphenol	95:104	arg1	PPO					115:117	PPO	115:117	PPO	115:117	Polyphenol oxidase (PPO) was immobilized on chitosan/montmorillonite (CTS/MMT) and chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT) composites, respectively.					
32422267	1	30	theme	Polyphenol	95:104	arg1	oxidase					106:112	Polyphenol oxidase	95:112	Polyphenol oxidase (PPO)	95:118	Polyphenol oxidase (PPO) was immobilized on chitosan/montmorillonite (CTS/MMT) and chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT) composites, respectively.					
32422267	10	31	theme	immobilized	1551:1561	arg1	PPO					1563:1565	immobilized PPO	1551:1565	immobilized PPO	1551:1565	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	8	32	theme	optimal	1117:1123	arg1	ratio					1142:1146	The optimal enzyme/substrate ratio	1113:1146	The optimal enzyme/substrate ratio	1113:1146	The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than that of IPPO proved the higher catalytic efficiency.					
32422267	8	32	theme	optimal	1117:1123	arg1	lower					1182:1186	lower	1182:1186	lower	1182:1186	The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than that of IPPO proved the higher catalytic efficiency.					
32422267	10	33	dep	activity	1525:1532	arg1	the					1514:1516	the	1514:1516	the	1514:1516	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	9	34	with	comparison	1304:1313	arg1	PH					1320:1321	PH	1320:1321	PH	1320:1321	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	7	35	theme	immobilized	969:979	arg1	PPO					981:983	both immobilized PPO	964:983	both immobilized PPO	964:983	The results showed that, for both immobilized PPO, the optimal pH was 7, 6 and 5 for PH, 4-CP and 2,4-DCP, respectively, and the optimal temperature was 30 °C for all substrate.					
32422267	8	36	theme	reaction	1152:1159	arg1	time					1161:1164	reaction time	1152:1164	reaction time	1152:1164	The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than that of IPPO proved the higher catalytic efficiency.					
32422267	6	37	theme	phenolic	879:886	arg1	compounds					888:896	the phenolic compounds removal	875:904	the phenolic compounds removal	875:904	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	1	38	dep	chitosan/montmorillonite	139:162	arg1	composites					238:247	composites	238:247	composites	238:247	Polyphenol oxidase (PPO) was immobilized on chitosan/montmorillonite (CTS/MMT) and chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT) composites, respectively.					
32422267	5	39	theme	2,4-dichlorophenol	727:744	arg1	removal					677:683	the removal	673:683	the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution	673:774	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	2	40	theme	Taguchi	264:270	arg1	method					272:277	Taguchi method	264:277	Taguchi method	264:277	Taguchi method was applied to determine the optimal immobilization conditions for achieving the maximum enzyme activity.					
32422267	6	41	dep	ratio	826:830	arg1	enzyme/substrate					809:824	enzyme/substrate	809:824	enzyme/substrate	809:824	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	8	42	theme	catalytic	1224:1232	arg1	efficiency					1234:1243	the higher catalytic efficiency	1213:1243	the higher catalytic efficiency	1213:1243	The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than that of IPPO proved the higher catalytic efficiency.					
32422267	9	43	from	efficiency	1290:1299	arg1	comparison					1304:1313	comparison	1304:1313	comparison with PH	1304:1321	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	8	44	theme	higher	1217:1222	arg1	efficiency					1234:1243	the higher catalytic efficiency	1213:1243	the higher catalytic efficiency	1213:1243	The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than that of IPPO proved the higher catalytic efficiency.					
32422267	9	45	theme	immobilized	1332:1342	arg1	PPO					1344:1346	both immobilized PPO	1327:1346	both immobilized PPO	1327:1346	Chlorophenols showed the improved catalytic efficiency in comparison with PH for both immobilized PPO, while the effect of substrate chemical structure on the IPPO-Au properties was limited.					
32422267	5	46	theme	4-chlorophenol	701:714	arg1	removal					677:683	the removal	673:683	the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution	673:774	The bio-catalytic performance of immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution.					
32422267	6	47	theme	reaction	858:865	arg1	pH					792:793	pH	792:793	pH	792:793	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	6	47	theme	reaction	858:865	arg1	time					867:870	reaction time	858:870	reaction time	858:870	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	0	48	dep	Preparation	32:42	arg1	oxidase					23:29	Immobilized polyphenol oxidase	0:29	Immobilized polyphenol oxidase	0:29	Immobilized polyphenol oxidase: Preparation, optimization and oxidation of phenolic compounds.					
32422267	10	49	theme	enzyme	1518:1523	arg1	activity					1525:1532	enzyme activity	1518:1532	enzyme activity	1518:1532	The results suggested AuNPs presented in support played an important role on the enzyme activity and stability of immobilized PPO.					
32422267	2	50	theme	enzyme	368:373	arg1	activity					375:382	the maximum enzyme activity	356:382	the maximum enzyme activity	356:382	Taguchi method was applied to determine the optimal immobilization conditions for achieving the maximum enzyme activity.					
32422267	8	51	dep	ratio	1142:1146	arg1	enzyme/substrate					1125:1140	enzyme/substrate	1125:1140	enzyme/substrate	1125:1140	The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than that of IPPO proved the higher catalytic efficiency.					
32422267	7	52	theme	optimal	990:996	arg1	7					1005:1005	7	1005:1005	7	1005:1005	The results showed that, for both immobilized PPO, the optimal pH was 7, 6 and 5 for PH, 4-CP and 2,4-DCP, respectively, and the optimal temperature was 30 °C for all substrate.					
32422267	7	52	theme	optimal	990:996	arg1	pH					998:999	the optimal pH	986:999	the optimal pH	986:999	The results showed that, for both immobilized PPO, the optimal pH was 7, 6 and 5 for PH, 4-CP and 2,4-DCP, respectively, and the optimal temperature was 30 °C for all substrate.					
32422267	2	53	theme	maximum	360:366	arg1	activity					375:382	the maximum enzyme activity	356:382	the maximum enzyme activity	356:382	Taguchi method was applied to determine the optimal immobilization conditions for achieving the maximum enzyme activity.					
32422267	6	54	from	effects	781:787	arg1	compounds					888:896	the phenolic compounds removal	875:904	the phenolic compounds removal	875:904	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	6	55	theme	substrate	833:841	arg1	concentration					843:855	substrate concentration	833:855	substrate concentration	833:855	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
32422267	6	55	theme	substrate	833:841	arg1	pH					792:793	pH	792:793	pH	792:793	The effects of pH, temperature, enzyme/substrate ratio, substrate concentration, reaction time on the phenolic compounds removal were investigated in detail.					
33259143	8	0	theme	gelatinization	1195:1208	arg1	GT					1223:1224	GT	1223:1224	GT	1223:1224	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	0	theme	gelatinization	1195:1208	arg1	temperature					1210:1220	lower starch gelatinization temperature	1182:1220	lower starch gelatinization temperature (GT)	1182:1225	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	1	1	theme	Africa	127:132	arg1	parts					118:122	many parts	113:122	many parts of Africa and Asia	113:141	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	7	2	theme	branching	961:969	arg1	precursor					980:988	a starch branching enzyme I precursor	952:988	a starch branching enzyme I precursor	952:988	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	9	3	theme	food	1358:1361	arg1	products					1363:1370	food products	1358:1370	food products that require good gel consistency and viscosity	1358:1418	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	0	4	from	diversity	8:16	arg1	sorghum					67:73	sorghum	67:73	sorghum	67:73	Genetic diversity for starch quality and alkali spreading value in sorghum.					
33259143	3	5	from	variation	443:451	arg1	quality					463:469	starch quality	456:469	starch quality based on alkali spreading value (ASV)	456:507	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	6	6	theme	lines	779:783	arg1	resequencing					752:763	Whole genome resequencing	739:763	Whole genome resequencing of ASV+ donor lines	739:783	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	3	7	theme	sorghum	307:313	arg1	lines					324:328	diverse sorghum breeding lines	299:328	diverse sorghum breeding lines	299:328	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	6	8	theme	Whole	739:743	arg1	resequencing					752:763	Whole genome resequencing	739:763	Whole genome resequencing of ASV+ donor lines	739:783	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	3	9	theme	alkali	480:485	arg1	ASV					504:506	ASV	504:506	ASV	504:506	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	3	9	theme	alkali	480:485	arg1	value					497:501	alkali spreading value	480:501	alkali spreading value (ASV)	480:507	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	9	10	theme	lines	1331:1335	arg1	valuable					1346:1353	valuable	1346:1353	valuable	1346:1353	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	9	10	theme	lines	1331:1335	arg1	attributes					1311:1320	The starch quality attributes	1292:1320	The starch quality attributes of these lines	1292:1335	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	3	11	theme	breeding	315:322	arg1	lines					324:328	diverse sorghum breeding lines	299:328	diverse sorghum breeding lines	299:328	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	6	12	theme	starch	820:825	arg1	biosynthesis					827:838	starch biosynthesis	820:838	starch biosynthesis	820:838	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	3	13	theme	spreading	487:495	arg1	ASV					504:506	ASV	504:506	ASV	504:506	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	3	13	theme	spreading	487:495	arg1	value					497:501	alkali spreading value	480:501	alkali spreading value (ASV)	480:507	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	2	14	from	variation	186:194	arg1	content					248:254	protein content	240:254	protein content	240:254	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	2	14	from	variation	186:194	arg1	traits					212:217	food quality traits	199:217	food quality traits including starch and protein content and composition	199:270	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	2	14	from	variation	186:194	arg1	starch					229:234	starch	229:234	starch	229:234	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	4	15	theme	small	512:516	arg1	number					518:523	A small number	510:523	A small number of genotypes with stable expression of the ASV+ phenotype across seasons	510:596	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	8	16	theme	ASV	1129:1131	arg1	phenotypes					1133:1142	contrasting ASV phenotypes	1117:1142	contrasting ASV phenotypes	1117:1142	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	2	17	theme	protein	240:246	arg1	content					248:254	protein content	240:254	protein content	240:254	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	1	18	theme	Asia	138:141	arg1	parts					118:122	many parts	113:122	many parts of Africa and Asia	113:141	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	3	19	theme	diverse	299:305	arg1	lines					324:328	diverse sorghum breeding lines	299:328	diverse sorghum breeding lines	299:328	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	8	20	with	accessions	1101:1110	arg1	phenotypes					1133:1142	contrasting ASV phenotypes	1117:1142	contrasting ASV phenotypes	1117:1142	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	3	21	theme	sorghum	338:344	arg1	SC					358:359	SC	358:359	SC	358:359	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	3	21	theme	sorghum	338:344	arg1	conversion					346:355	788 sorghum conversion	334:355	788 sorghum conversion (SC) lines	334:366	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	3	22	theme	global	385:390	arg1	diversity					402:410	the global germplasm diversity	381:410	the global germplasm diversity of the crop	381:422	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	9	23	from	products	1363:1370	arg1	valuable					1346:1353	valuable	1346:1353	valuable	1346:1353	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	9	23	from	products	1363:1370	arg1	attributes					1311:1320	The starch quality attributes	1292:1320	The starch quality attributes of these lines	1292:1335	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	7	24	theme	enzyme	971:976	arg1	precursor					980:988	a starch branching enzyme I precursor	952:988	a starch branching enzyme I precursor	952:988	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	3	25	theme	lines	362:366	arg1	panel					290:294	a panel	288:294	a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop	288:422	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	0	26	theme	Genetic	0:6	arg1	diversity					8:16	Genetic diversity	0:16	Genetic diversity for starch quality and alkali spreading value in sorghum.	0:74	Genetic diversity for starch quality and alkali spreading value in sorghum.					
33259143	7	27	theme	wide	850:853	arg1	study					867:871	A genome wide association study	841:871	A genome wide association study (GWAS)	841:878	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	7	27	theme	wide	850:853	arg1	GWAS					874:877	GWAS	874:877	GWAS	874:877	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	0	28	theme	starch	22:27	arg1	quality					29:35	starch quality	22:35	starch quality	22:35	Genetic diversity for starch quality and alkali spreading value in sorghum.					
33259143	8	29	theme	contrasting	1117:1127	arg1	phenotypes					1133:1142	contrasting ASV phenotypes	1117:1142	contrasting ASV phenotypes	1117:1142	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	1	30	from	crop	105:108	arg1	parts					118:122	many parts	113:122	many parts of Africa and Asia	113:141	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	8	31	theme	gel	1275:1277	arg1	consistency					1279:1289	good gel consistency	1270:1289	good gel consistency	1270:1289	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	6	32	theme	genome	745:750	arg1	resequencing					752:763	Whole genome resequencing	739:763	Whole genome resequencing of ASV+ donor lines	739:783	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	8	33	theme	amylose	1228:1234	arg1	content					1236:1242	amylose content	1228:1242	amylose content of approximately 22%	1228:1263	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	6	34	from	SNPs	794:797	arg1	genes					802:806	genes	802:806	genes involved in starch biosynthesis	802:838	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	8	35	theme	accessions	1101:1110	arg1	analyses					1089:1096	Physiochemical analyses	1074:1096	Physiochemical analyses of accessions with contrasting ASV phenotypes	1074:1142	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	1	36	theme	important	90:98	arg1	crop					105:108	an important food crop	87:108	an important food crop in many parts of Africa and Asia	87:141	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	1	36	theme	important	90:98	arg1	Sorghum					76:82	Sorghum	76:82	Sorghum	76:82	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	8	37	theme	good	1270:1273	arg1	consistency					1279:1289	good gel consistency	1270:1289	good gel consistency	1270:1289	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	38	theme	lower	1182:1186	arg1	GT					1223:1224	GT	1223:1224	GT	1223:1224	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	38	theme	lower	1182:1186	arg1	temperature					1210:1220	lower starch gelatinization temperature	1182:1220	lower starch gelatinization temperature (GT)	1182:1225	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	9	39	theme	quality	1303:1309	arg1	valuable					1346:1353	valuable	1346:1353	valuable	1346:1353	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	9	39	theme	quality	1303:1309	arg1	attributes					1311:1320	The starch quality attributes	1292:1320	The starch quality attributes of these lines	1292:1335	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	7	40	theme	I	978:978	arg1	precursor					980:988	a starch branching enzyme I precursor	952:988	a starch branching enzyme I precursor	952:988	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	4	41	theme	ASV+	568:571	arg1	phenotype					573:581	the ASV+ phenotype	564:581	the ASV+ phenotype	564:581	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	2	42	theme	food	199:202	arg1	content					248:254	protein content	240:254	protein content	240:254	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	2	42	theme	food	199:202	arg1	traits					212:217	food quality traits	199:217	food quality traits including starch and protein content and composition	199:270	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	2	42	theme	food	199:202	arg1	starch					229:234	starch	229:234	starch	229:234	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	3	43	theme	germplasm	392:400	arg1	diversity					402:410	the global germplasm diversity	381:410	the global germplasm diversity of the crop	381:422	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	8	44	theme	dependent	1172:1180	arg1	environment					1160:1170	an environment	1157:1170	an environment dependent lower starch gelatinization temperature (GT)	1157:1225	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	9	45	from	valuable	1346:1353	arg1	products					1363:1370	food products	1358:1370	food products that require good gel consistency and viscosity	1358:1418	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	8	46	dep	dependent	1172:1180	arg1	GT					1223:1224	GT	1223:1224	GT	1223:1224	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	46	dep	dependent	1172:1180	arg1	temperature					1210:1220	lower starch gelatinization temperature	1182:1220	lower starch gelatinization temperature (GT)	1182:1225	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	3	47	theme	lines	324:328	arg1	panel					290:294	a panel	288:294	a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop	288:422	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	8	48	theme	%	1263:1263	arg1	environment					1160:1170	an environment	1157:1170	an environment dependent lower starch gelatinization temperature (GT)	1157:1225	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	48	theme	%	1263:1263	arg1	content					1236:1242	amylose content	1228:1242	amylose content of approximately 22%	1228:1263	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	48	theme	%	1263:1263	arg1	consistency					1279:1289	good gel consistency	1270:1289	good gel consistency	1270:1289	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	0	49	theme	spreading	48:56	arg1	value					58:62	alkali spreading value	41:62	alkali spreading value	41:62	Genetic diversity for starch quality and alkali spreading value in sorghum.					
33259143	9	50	theme	gel	1390:1392	arg1	consistency					1394:1404	good gel consistency	1385:1404	good gel consistency	1385:1404	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	5	51	theme	recessive	722:730	arg1	trait					732:736	a recessive trait	720:736	a recessive trait	720:736	Genetic studies showed the ASV+ phenotype was inherited as a recessive trait.					
33259143	5	52	theme	ASV+	688:691	arg1	phenotype					693:701	the ASV+ phenotype	684:701	the ASV+ phenotype	684:701	Genetic studies showed the ASV+ phenotype was inherited as a recessive trait.					
33259143	4	53	with	genotypes	528:536	arg1	expression					550:559	stable expression	543:559	stable expression of the ASV+ phenotype across seasons	543:596	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	9	54	theme	good	1385:1388	arg1	consistency					1394:1404	good gel consistency	1385:1404	good gel consistency	1385:1404	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	1	55	theme	food	100:103	arg1	crop					105:108	an important food crop	87:108	an important food crop in many parts of Africa and Asia	87:141	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	1	55	theme	food	100:103	arg1	Sorghum					76:82	Sorghum	76:82	Sorghum	76:82	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	0	56	theme	alkali	41:46	arg1	value					58:62	alkali spreading value	41:62	alkali spreading value	41:62	Genetic diversity for starch quality and alkali spreading value in sorghum.					
33259143	5	57	dep	showed	677:682	arg1	inherited					707:715	inherited	707:715	showed the ASV+ phenotype was inherited as a recessive trait	677:736	Genetic studies showed the ASV+ phenotype was inherited as a recessive trait.					
33259143	6	58	theme	donor	773:777	arg1	lines					779:783	ASV+ donor lines	768:783	ASV+ donor lines	768:783	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	4	59	theme	Nandyal	635:641	arg1	types					643:647	Nandyal types	635:647	Nandyal types from India	635:658	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	4	60	from	India	654:658	arg1	types					643:647	Nandyal types	635:647	Nandyal types from India	635:658	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	7	61	theme	association	855:865	arg1	study					867:871	A genome wide association study	841:871	A genome wide association study (GWAS)	841:878	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	7	61	theme	association	855:865	arg1	GWAS					874:877	GWAS	874:877	GWAS	874:877	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	6	62	theme	ASV+	768:771	arg1	lines					779:783	ASV+ donor lines	768:783	ASV+ donor lines	768:783	Whole genome resequencing of ASV+ donor lines revealed SNPs in genes involved in starch biosynthesis.					
33259143	9	63	theme	starch	1296:1301	arg1	valuable					1346:1353	valuable	1346:1353	valuable	1346:1353	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	9	63	theme	starch	1296:1301	arg1	attributes					1311:1320	The starch quality attributes	1292:1320	The starch quality attributes of these lines	1292:1335	The starch quality attributes of these lines could be valuable in food products that require good gel consistency and viscosity.					
33259143	3	64	theme	crop	419:422	arg1	diversity					402:410	the global germplasm diversity	381:410	the global germplasm diversity of the crop	381:422	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	7	65	theme	genome	843:848	arg1	study					867:871	A genome wide association study	841:871	A genome wide association study (GWAS)	841:878	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	7	65	theme	genome	843:848	arg1	GWAS					874:877	GWAS	874:877	GWAS	874:877	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	4	66	theme	genotypes	528:536	arg1	number					518:523	A small number	510:523	A small number of genotypes with stable expression of the ASV+ phenotype across seasons	510:596	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	7	67	theme	starch	954:959	arg1	precursor					980:988	a starch branching enzyme I precursor	952:988	a starch branching enzyme I precursor	952:988	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	8	68	theme	starch	1188:1193	arg1	GT					1223:1224	GT	1223:1224	GT	1223:1224	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	8	68	theme	starch	1188:1193	arg1	temperature					1210:1220	lower starch gelatinization temperature	1182:1220	lower starch gelatinization temperature (GT)	1182:1225	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	1	69	theme	many	113:116	arg1	parts					118:122	many parts	113:122	many parts of Africa and Asia	113:141	Sorghum is an important food crop in many parts of Africa and Asia.					
33259143	7	70	dep	identified	880:889	arg1	both					1034:1037	both	1034:1037	both	1034:1037	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	8	71	theme	Physiochemical	1074:1087	arg1	analyses					1089:1096	Physiochemical analyses	1074:1096	Physiochemical analyses of accessions with contrasting ASV phenotypes	1074:1142	Physiochemical analyses of accessions with contrasting ASV phenotypes demonstrated an environment dependent lower starch gelatinization temperature (GT), amylose content of approximately 22%, and good gel consistency.					
33259143	2	72	theme	sorghum	157:163	arg1	Landraces					144:152	Landraces	144:152	Landraces of sorghum	144:163	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	3	73	theme	starch	456:461	arg1	quality					463:469	starch quality	456:469	starch quality based on alkali spreading value (ASV)	456:507	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	4	74	theme	phenotype	573:581	arg1	expression					550:559	stable expression	543:559	stable expression of the ASV+ phenotype across seasons	543:596	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	4	75	theme	stable	543:548	arg1	expression					550:559	stable expression	543:559	stable expression of the ASV+ phenotype across seasons	543:596	A small number of genotypes with stable expression of the ASV+ phenotype across seasons were identified; mostly representing Nandyal types from India.					
33259143	3	76	theme	conversion	346:355	arg1	lines					362:366	788 sorghum conversion (SC) lines	334:366	788 sorghum conversion (SC) lines	334:366	In this study, a panel of diverse sorghum breeding lines and 788 sorghum conversion (SC) lines representing the global germplasm diversity of the crop were evaluated for variation in starch quality based on alkali spreading value (ASV).					
33259143	2	77	theme	quality	204:210	arg1	content					248:254	protein content	240:254	protein content	240:254	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	2	77	theme	quality	204:210	arg1	traits					212:217	food quality traits	199:217	food quality traits including starch and protein content and composition	199:270	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	2	77	theme	quality	204:210	arg1	starch					229:234	starch	229:234	starch	229:234	Landraces of sorghum are known to exhibit variation in food quality traits including starch and protein content and composition.					
33259143	7	78	theme	significant	893:903	arg1	SNP					905:907	a significant SNP	891:907	a significant SNP associated with ASV	891:927	A genome wide association study (GWAS) identified a significant SNP associated with ASV near Sobic.010G273800, a starch branching enzyme I precursor, and Sobic.010G274800 and Sobic.010G275001, both annotated as glucosyltransferases.					
33259143	5	79	theme	Genetic	661:667	arg1	studies					669:675	Genetic studies	661:675	Genetic studies	661:675	Genetic studies showed the ASV+ phenotype was inherited as a recessive trait.					
34579912	3	0	theme	3D	511:512	arg1	bioprinting					514:524	3D bioprinting	511:524	3D bioprinting	511:524	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	4	1	theme	scaffold	812:819	arg1	shape					799:803	the inherent shape	786:803	the inherent shape of the scaffold	786:819	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	13	2	contain	has	2056:2058	arg1	scaffold					2047:2054	This 3D-bioprinted composite scaffold	2018:2054	This 3D-bioprinted composite scaffold	2018:2054	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	13	2	contain	has	2056:2058	arg2	biocompatibility					2065:2080	good biocompatibility	2060:2080	good biocompatibility	2060:2080	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	10	3	dep	%	1648:1648	arg1	%					1638:1638	%	1638:1638	%	1638:1638	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	1	4	theme	damage	193:198	arg1	degrees					182:188	various degrees	174:188	various degrees of damage to the morphological structure and physiological function of the peripheral nerve	174:280	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	10	5	from	neurofactors	1570:1581	arg1	group					1653:1657	the 5%GelMA+0.3%BNC group	1633:1657	the 5%GelMA+0.3%BNC group	1633:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	6	6	theme	mass	1118:1121	arg1	death					1128:1132	no mass cell death	1115:1132	no mass cell death	1115:1132	Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing.					
34579912	10	7	dep	group	1653:1657	arg1	%					1648:1648	%	1648:1648	%	1648:1648	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	2	8	theme	"	323:323	arg1	transplantation					342:356	"gold standard" autologous nerve transplantation	309:356	"gold standard" autologous nerve transplantation	309:356	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	13	9	theme	tissue	2163:2168	arg1	engineering					2170:2180	neural tissue engineering	2156:2180	neural tissue engineering	2156:2180	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	12	10	theme	composite	1917:1925	arg1	scaffold					1927:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	4	11	contain	had	714:716	arg2	lines					731:735	the thinnest lines	718:735	the thinnest lines among all groups	718:752	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	4	11	contain	had	714:716	arg1	group					708:712	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	5	12	theme	BNC	1019:1021	arg1	group					1023:1027	the 5% alginate+5% GelMA+0.3% BNC group	989:1027	the 5% alginate+5% GelMA+0.3% BNC group	989:1027	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	3	13	theme	RSC96	559:563	arg1	cells					565:569	RSC96 cells	559:569	RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	559:664	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	8	14	from	obvious	1308:1314	arg1	group					1353:1357	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	13	15	theme	material	2131:2138	arg1	type					2114:2117	a new type	2108:2117	a new type of scaffold material	2108:2138	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	13	16	theme	new	2110:2112	arg1	type					2114:2117	a new type	2108:2117	a new type of scaffold material	2108:2138	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	10	17	theme	Polymerase	1489:1498	arg1	PCR					1516:1518	PCR	1516:1518	PCR	1516:1518	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	17	theme	Polymerase	1489:1498	arg1	reaction					1506:1513	Polymerase chain reaction	1489:1513	Polymerase chain reaction (PCR) array results	1489:1533	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	18	theme	5	1637:1637	arg1	%					1638:1638	%	1638:1638	%	1638:1638	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	1	19	theme	physiological	235:247	arg1	function					249:256	physiological function	235:256	physiological function	235:256	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	8	20	theme	alginate+5	1326:1335	arg1	%					1347:1347	the 5% alginate+5% GelMA+0.3%	1319:1347	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	4	21	theme	%	702:702	arg1	group					708:712	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	5	22	theme	alginate+5	996:1005	arg1	%					994:994	%	994:994	%	994:994	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	1	23	theme	Peripheral	140:149	arg1	injury					157:162	Peripheral nerve injury	140:162	Peripheral nerve injury	140:162	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	12	24	theme	sodium	1891:1896	arg1	scaffold					1927:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	5	25	theme	GelMA+0.3	1008:1016	arg1	%					1017:1017	%	1017:1017	%	1017:1017	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	3	26	theme	/bacterial	626:635	arg1	hydrogel					657:664	sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	582:664	sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	582:664	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	8	27	theme	BNC	1349:1351	arg1	group					1353:1357	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	4	28	theme	sodium	674:679	arg1	%					702:702	The 5% sodium alginate+5% GelMA+0.3%	667:702	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	0	29	theme	BNC	86:88	arg1	scaffold					91:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	10	30	theme	GelMA+0.3	1639:1647	arg1	%					1638:1638	%	1638:1638	%	1638:1638	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	5	31	theme	Physical	859:866	arg1	testing					890:896	Physical and chemical property testing	859:896	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus)	859:975	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	11	32	from	implantation	1716:1727	arg1	mice					1737:1740	nude mice	1732:1740	nude mice	1732:1740	Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive.					
34579912	4	33	theme	%	691:691	arg1	%					702:702	The 5% sodium alginate+5% GelMA+0.3%	667:702	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	0	34	theme	scaffold	91:98	arg1	evaluation					17:26	Biocompatibility evaluation	0:26	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	0:98	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	5	35	theme	chemical	872:879	arg1	testing					890:896	Physical and chemical property testing	859:896	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus)	859:975	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	10	36	theme	BNC	1649:1651	arg1	group					1653:1657	the 5%GelMA+0.3%BNC group	1633:1657	the 5%GelMA+0.3%BNC group	1633:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	37	theme	neurofactors	1570:1581	arg1	levels					1552:1557	mRNA levels	1547:1557	mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group	1547:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	5	38	theme	mechanical	1040:1049	arg1	properties					1067:1076	better mechanical and rheological properties	1033:1076	better mechanical and rheological properties	1033:1076	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	9	39	theme	BNC	1393:1395	arg1	group					1397:1401	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	12	40	theme	3D-bioprinted	1877:1889	arg1	scaffold					1927:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	0	41	theme	RSC96	127:131	arg1	cells					133:137	oriented-growth RSC96 cells	111:137	oriented-growth RSC96 cells	111:137	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	5	42	theme	rheological	1055:1065	arg1	properties					1067:1076	better mechanical and rheological properties	1033:1076	better mechanical and rheological properties	1033:1076	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	9	43	theme	%	1380:1380	arg1	%					1391:1391	the 5% alginate+5% GelMA+0.3%	1363:1391	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	0	44	theme	Biocompatibility	0:15	arg1	evaluation					17:26	Biocompatibility evaluation	0:26	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	0:98	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	9	45	theme	immunofluorescence	1411:1428	arg1	staining					1430:1437	S-100β immunofluorescence staining	1404:1437	S-100β immunofluorescence staining	1404:1437	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	7	46	dep	group	1214:1218	arg1	%					1206:1206	%	1206:1206	%	1206:1206	In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold.					
34579912	2	47	theme	achieving	438:446	arg1	challenge					477:485	a challenge	475:485	a challenge	475:485	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	2	47	theme	achieving	438:446	arg1	guidance					457:464	achieving oriented guidance	438:464	achieving oriented guidance	438:464	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	10	48	from	group	1653:1657	arg1	levels					1552:1557	mRNA levels	1547:1557	mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group	1547:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	7	49	theme	GelMA	1208:1212	arg1	group					1214:1218	the 5% alginate+5% GelMA group	1189:1218	the 5% alginate+5% GelMA group	1189:1218	In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold.					
34579912	4	50	theme	inherent	790:797	arg1	shape					799:803	the inherent shape	786:803	the inherent shape of the scaffold	786:819	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	0	51	theme	3D-bioprinted	33:45	arg1	scaffold					91:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	11	52	dep	weeks	1704:1708	arg1	implantation					1716:1727	implantation	1716:1727	implantation in nude mice	1716:1740	Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive.					
34579912	7	53	theme	alginate+5	1196:1205	arg1	%					1194:1194	%	1194:1194	%	1194:1194	In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold.					
34579912	2	54	theme	tissue	359:364	arg1	engineering					366:376	tissue engineering	359:376	tissue engineering	359:376	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	0	55	theme	nanocellulose	71:83	arg1	scaffold					91:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	13	56	theme	composite	2037:2045	arg1	scaffold					2047:2054	This 3D-bioprinted composite scaffold	2018:2054	This 3D-bioprinted composite scaffold	2018:2054	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	9	57	theme	oriented	1472:1479	arg1	growth					1481:1486	oriented growth	1472:1486	oriented growth	1472:1486	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	6	58	theme	cell	1089:1092	arg1	staining					1094:1101	Live/dead cell staining	1079:1101	Live/dead cell staining	1079:1101	Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing.					
34579912	9	59	theme	alginate+5	1370:1379	arg1	%					1391:1391	the 5% alginate+5% GelMA+0.3%	1363:1391	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	11	60	theme	nude	1732:1735	arg1	mice					1737:1740	nude mice	1732:1740	nude mice	1732:1740	Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive.					
34579912	5	61	dep	group	1023:1027	arg1	%					1006:1006	%	1006:1006	%	1006:1006	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	1	62	theme	nerve	276:280	arg1	structure					221:229	morphological structure	207:229	morphological structure	207:229	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	1	62	theme	nerve	276:280	arg1	function					249:256	physiological function	235:256	physiological function	235:256	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	10	63	from	levels	1552:1557	arg1	group					1653:1657	the 5%GelMA+0.3%BNC group	1633:1657	the 5%GelMA+0.3%BNC group	1633:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	6	64	located	observed	1138:1145	arg2	death					1128:1132	no mass cell death	1115:1132	no mass cell death	1115:1132	Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing.					
34579912	6	64	located	observed	1138:1145	arg1	days					1150:1153	days 1, 3, 5, and 7	1150:1168	days 1, 3, 5, and 7	1150:1168	Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing.					
34579912	3	65	theme	nerve	541:545	arg1	scaffold					547:554	a nerve scaffold	539:554	a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	539:664	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	2	66	theme	autologous	325:334	arg1	transplantation					342:356	"gold standard" autologous nerve transplantation	309:356	"gold standard" autologous nerve transplantation	309:356	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	5	67	dep	transform	907:915	arg1	infrared					917:924	infrared	917:924	transform infrared	907:924	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	5	68	dep	%	1006:1006	arg1	%					994:994	%	994:994	%	994:994	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	10	69	theme	related	1562:1568	arg1	ASCL1					1583:1587	ASCL1	1583:1587	ASCL1	1583:1587	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	69	theme	related	1562:1568	arg1	POU3F3					1590:1595	POU3F3	1590:1595	POU3F3	1590:1595	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	69	theme	related	1562:1568	arg1	NOTCH1					1613:1618	NOTCH1	1613:1618	NOTCH1	1613:1618	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	69	theme	related	1562:1568	arg1	ERBB2					1624:1628	ERBB2	1624:1628	ERBB2	1624:1628	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	69	theme	related	1562:1568	arg1	NEUROG1					1598:1604	NEUROG1	1598:1604	NEUROG1	1598:1604	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	69	theme	related	1562:1568	arg1	DLL1					1607:1610	DLL1	1607:1610	DLL1	1607:1610	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	69	theme	related	1562:1568	arg1	neurofactors					1570:1581	related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2	1562:1628	related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group	1562:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	6	70	theme	cell	1123:1126	arg1	death					1128:1132	no mass cell death	1115:1132	no mass cell death	1115:1132	Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing.					
34579912	3	71	theme	cells	565:569	arg1	scaffold					547:554	a nerve scaffold	539:554	a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	539:664	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	13	72	theme	neural	2156:2161	arg1	engineering					2170:2180	neural tissue engineering	2156:2180	neural tissue engineering	2156:2180	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	2	73	theme	standard	315:322	arg1	"					323:323	"gold standard"	309:323	"gold standard" autologous nerve transplantation	309:356	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	1	74	theme	morphological	207:219	arg1	structure					221:229	morphological structure	207:229	morphological structure	207:229	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	8	75	theme	%	1324:1324	arg1	%					1347:1347	the 5% alginate+5% GelMA+0.3%	1319:1347	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	12	76	theme	alginate/GelMA/BNC	1898:1915	arg1	scaffold					1927:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold	1875:1934	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	10	77	theme	chain	1500:1504	arg1	PCR					1516:1518	PCR	1516:1518	PCR	1516:1518	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	77	theme	chain	1500:1504	arg1	reaction					1506:1513	Polymerase chain reaction	1489:1513	Polymerase chain reaction (PCR) array results	1489:1533	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	7	78	theme	5	1193:1193	arg1	%					1194:1194	%	1194:1194	%	1194:1194	In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold.					
34579912	8	79	theme	%	1336:1336	arg1	%					1347:1347	the 5% alginate+5% GelMA+0.3%	1319:1347	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	8	80	from	group	1353:1357	arg1	obvious					1308:1314	obvious	1308:1314	obvious	1308:1314	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	11	81	theme	RSC96	1743:1747	arg1	cells					1749:1753	RSC96 cells	1743:1753	RSC96 cells	1743:1753	Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive.					
34579912	5	82	theme	property	881:888	arg1	testing					890:896	Physical and chemical property testing	859:896	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus)	859:975	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	8	83	theme	%	1347:1347	arg1	group					1353:1357	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	the 5% alginate+5% GelMA+0.3% BNC group	1319:1357	This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group.					
34579912	13	84	theme	engineering	2170:2180	arg1	field					2147:2151	the field	2143:2151	the field of neural tissue engineering	2143:2180	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	1	85	theme	nerve	151:155	arg1	injury					157:162	Peripheral nerve injury	140:162	Peripheral nerve injury	140:162	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	11	86	theme	S-100β	1807:1812	arg1	immunofluorescence					1814:1831	S-100β immunofluorescence	1807:1831	S-100β immunofluorescence	1807:1831	Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive.					
34579912	7	87	theme	linear	1247:1252	arg1	connections					1254:1264	linear connections	1247:1264	linear connections	1247:1264	In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold.					
34579912	4	88	theme	BNC	704:706	arg1	group					708:712	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	5	89	theme	%	1017:1017	arg1	group					1023:1027	the 5% alginate+5% GelMA+0.3% BNC group	989:1027	the 5% alginate+5% GelMA+0.3% BNC group	989:1027	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	11	90	theme	blood	1783:1787	arg1	vessels					1789:1795	blood vessels	1783:1795	blood vessels	1783:1795	Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive.					
34579912	9	91	theme	%	1368:1368	arg1	%					1391:1391	the 5% alginate+5% GelMA+0.3%	1363:1391	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	4	92	theme	%	672:672	arg1	%					702:702	The 5% sodium alginate+5% GelMA+0.3%	667:702	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	12	93	theme	related	2001:2007	arg1	factors					2009:2015	related factors	2001:2015	related factors	2001:2015	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	1	94	theme	various	174:180	arg1	degrees					182:188	various degrees	174:188	various degrees of damage to the morphological structure and physiological function of the peripheral nerve	174:280	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	4	95	theme	alginate+5	681:690	arg1	%					702:702	The 5% sodium alginate+5% GelMA+0.3%	667:702	The 5% sodium alginate+5% GelMA+0.3% BNC group	667:712	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	0	96	theme	oriented-growth	111:125	arg1	cells					133:137	oriented-growth RSC96 cells	111:137	oriented-growth RSC96 cells	111:137	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	9	97	theme	%	1391:1391	arg1	group					1397:1401	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	the 5% alginate+5% GelMA+0.3% BNC group	1363:1401	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	2	98	theme	gold	310:313	arg1	"					323:323	"gold standard"	309:323	"gold standard" autologous nerve transplantation	309:356	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	ASCL1					1583:1587	ASCL1	1583:1587	ASCL1	1583:1587	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	POU3F3					1590:1595	POU3F3	1590:1595	POU3F3	1590:1595	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	NOTCH1					1613:1618	NOTCH1	1613:1618	NOTCH1	1613:1618	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	ERBB2					1624:1628	ERBB2	1624:1628	ERBB2	1624:1628	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	NEUROG1					1598:1604	NEUROG1	1598:1604	NEUROG1	1598:1604	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	DLL1					1607:1610	DLL1	1607:1610	DLL1	1607:1610	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	10	99	dep	neurofactors	1570:1581	arg1	neurofactors					1570:1581	related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2	1562:1628	related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group	1562:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	5	100	dep	Fourier	899:905	arg1	conductivity					938:949	conductivity	938:949	conductivity	938:949	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	5	100	dep	Fourier	899:905	arg1	transform					907:915	transform	907:915	transform infrared	907:924	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	5	100	dep	Fourier	899:905	arg1	rheometer					927:935	rheometer	927:935	rheometer	927:935	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	5	100	dep	Fourier	899:905	arg1	modulus					968:974	compression modulus	956:974	compression modulus	956:974	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	10	101	theme	mRNA	1547:1550	arg1	levels					1552:1557	mRNA levels	1547:1557	mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group	1547:1657	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	2	102	contain	has	378:380	arg2	potential					390:398	certain potential	382:398	certain potential for regeneration and growth	382:426	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	2	102	contain	has	378:380	arg1	engineering					366:376	tissue engineering	359:376	tissue engineering	359:376	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	5	103	dep	testing	890:896	arg1	Fourier					899:905	Fourier	899:905	Fourier transform infrared	899:924	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	9	104	theme	S-100β	1404:1409	arg1	staining					1430:1437	S-100β immunofluorescence staining	1404:1437	S-100β immunofluorescence staining	1404:1437	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	5	105	contain	had	1029:1031	arg1	group					1023:1027	the 5% alginate+5% GelMA+0.3% BNC group	989:1027	the 5% alginate+5% GelMA+0.3% BNC group	989:1027	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	5	105	contain	had	1029:1031	arg2	properties					1067:1076	better mechanical and rheological properties	1033:1076	better mechanical and rheological properties	1033:1076	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	10	106	theme	reaction	1506:1513	arg1	results					1527:1533	Polymerase chain reaction (PCR) array results	1489:1533	Polymerase chain reaction (PCR) array results	1489:1533	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	13	107	theme	good	2060:2063	arg1	biocompatibility					2065:2080	good biocompatibility	2060:2080	good biocompatibility	2060:2080	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	2	108	theme	oriented	448:455	arg1	challenge					477:485	a challenge	475:485	a challenge	475:485	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	2	108	theme	oriented	448:455	arg1	guidance					457:464	achieving oriented guidance	438:464	achieving oriented guidance	438:464	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	3	109	theme	BNC	652:654	arg1	hydrogel					657:664	sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	582:664	sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	582:664	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	4	110	theme	thinnest	722:729	arg1	lines					731:735	the thinnest lines	718:735	the thinnest lines among all groups	718:752	The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC.					
34579912	0	111	theme	alginate-GelMA-bacteria	47:69	arg1	scaffold					91:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold	31:98	Biocompatibility evaluation of a 3D-bioprinted alginate-GelMA-bacteria nanocellulose (BNC) scaffold laden with oriented-growth RSC96 cells.					
34579912	9	112	theme	cytoskeleton	1443:1454	arg1	staining					1456:1463	cytoskeleton staining	1443:1463	cytoskeleton staining	1443:1463	In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth.					
34579912	3	113	theme	nanocellulose	637:649	arg1	hydrogel					657:664	sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	582:664	sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel	582:664	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	10	114	theme	array	1521:1525	arg1	results					1527:1533	Polymerase chain reaction (PCR) array results	1489:1533	Polymerase chain reaction (PCR) array results	1489:1533	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	13	115	theme	3D-bioprinted	2023:2035	arg1	scaffold					2047:2054	This 3D-bioprinted composite scaffold	2018:2054	This 3D-bioprinted composite scaffold	2018:2054	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	5	116	theme	5	993:993	arg1	%					994:994	%	994:994	%	994:994	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	12	117	theme	factors	2009:2015	arg1	adhesion					1970:1977	adhesion	1970:1977	adhesion	1970:1977	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	12	117	theme	factors	2009:2015	arg1	expression					1987:1996	the expression	1983:1996	the expression of related factors	1983:2015	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	12	117	theme	factors	2009:2015	arg1	growth					1962:1967	cell-oriented growth	1948:1967	cell-oriented growth	1948:1967	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	13	118	theme	scaffold	2122:2129	arg1	material					2131:2138	scaffold material	2122:2138	scaffold material	2122:2138	This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.					
34579912	5	119	theme	compression	956:966	arg1	modulus					968:974	compression modulus	956:974	compression modulus	956:974	Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties.					
34579912	6	120	theme	Live/dead	1079:1087	arg1	staining					1094:1101	Live/dead cell staining	1079:1101	Live/dead cell staining	1079:1101	Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing.					
34579912	10	121	theme	other	1685:1689	arg1	groups					1691:1696	other groups	1685:1696	other groups	1685:1696	Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups.					
34579912	3	122	used	used	506:509	arg2	we					503:504	we	503:504	we	503:504	In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel.					
34579912	2	123	theme	nerve	336:340	arg1	transplantation					342:356	"gold standard" autologous nerve transplantation	309:356	"gold standard" autologous nerve transplantation	309:356	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	12	124	theme	cell-oriented	1948:1960	arg1	growth					1962:1967	cell-oriented growth	1948:1967	cell-oriented growth	1948:1967	These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors.					
34579912	7	125	dep	%	1206:1206	arg1	%					1194:1194	%	1194:1194	%	1194:1194	In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold.					
34579912	1	126	dep	structure	221:229	arg1	the					203:205	the	203:205	the	203:205	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
34579912	2	127	theme	certain	382:388	arg1	potential					390:398	certain potential	382:398	certain potential for regeneration and growth	382:426	At present, compared with "gold standard" autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge.					
34579912	1	128	theme	peripheral	265:274	arg1	nerve					276:280	the peripheral nerve	261:280	the peripheral nerve	261:280	Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve.					
32797046	4	0	theme	α-proteobacterial	892:908	arg1	species					910:916	a related α-proteobacterial species	882:916	a related α-proteobacterial species	882:916	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	4	0	theme	α-proteobacterial	892:908	arg1	crescentus					931:940	Caulobacter crescentus	919:940	Caulobacter crescentus	919:940	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	10	1	theme	natural	2072:2078	arg1	response					2080:2087	a natural response	2070:2087	a natural response of Z. mobilis to sucrose that promotes sucrase production	2070:2145	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	2	2	theme	many	380:383	arg1	microorganisms					396:409	many industrial microorganisms	380:409	many industrial microorganisms	380:409	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	1	3	contain	have	179:182	arg1	chemicals					146:154	chemicals	146:154	chemicals	146:154	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	1	3	contain	have	179:182	arg1	fuels					136:140	Plant-derived fuels	122:140	Plant-derived fuels	122:140	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	1	3	contain	have	179:182	arg2	potential					196:204	significant potential	184:204	significant potential	184:204	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	2	4	theme	significant	306:316	arg1	coli					434:437	Escherichia coli	422:437	Escherichia coli	422:437	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	4	theme	significant	306:316	arg1	cerevisiae					454:463	Saccharomyces cerevisiae	440:463	Saccharomyces cerevisiae	440:463	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	4	theme	significant	306:316	arg1	fractions					318:326	significant fractions	306:326	significant fractions	306:326	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	4	theme	significant	306:316	arg1	mobilis					480:486	Zymomonas mobilis	470:486	Zymomonas mobilis	470:486	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	4	5	from	adaptation	966:975	arg1	medium					991:996	cellobiose medium	980:996	cellobiose medium	980:996	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	10	6	theme	mobilis	2095:2101	arg1	response					2080:2087	a natural response	2070:2087	a natural response of Z. mobilis to sucrose that promotes sucrase production	2070:2145	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	9	7	theme	cellobiose-	1697:1707	arg1	strains					1729:1735	cellobiose- and sucrose-adapted strains	1697:1735	cellobiose- and sucrose-adapted strains	1697:1735	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	7	8	theme	GH-expressing	1439:1451	arg1	strain					1453:1458	our GH-expressing strain	1435:1458	our GH-expressing strain	1435:1458	After adaptation to cellobiose, our GH-expressing strain was able to convert about 50% of cellobiose to glucose within 24 h and use it for growth and ethanol production.					
32797046	5	9	from	phase	1055:1059	arg1	medium					1075:1080	cellobiose medium	1064:1080	cellobiose medium	1064:1080	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	9	10	theme	sucrose-adapted	1713:1727	arg1	strains					1729:1735	cellobiose- and sucrose-adapted strains	1697:1735	cellobiose- and sucrose-adapted strains	1697:1735	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	6	11	theme	cells	1369:1373	arg1	transfer					1357:1364	transfer	1357:1364	transfer of cells to fresh cellobiose medium	1357:1400	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	3	12	theme	longer	619:624	arg1	oligomers					644:652	longer cellulose-derived oligomers	619:652	longer cellulose-derived oligomers	619:652	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	5	13	theme	gene	1106:1109	arg1	expression					1111:1120	gene expression	1106:1120	gene expression	1106:1120	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	5	14	theme	cellobiose	1064:1073	arg1	medium					1075:1080	cellobiose medium	1064:1080	cellobiose medium	1064:1080	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	1	15	from	reliance	217:224	arg1	petroleum					229:237	petroleum	229:237	petroleum	229:237	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	10	16	theme	sole	2009:2012	arg1	source					2021:2026	a sole carbon source	2007:2026	a sole carbon source	2007:2026	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	8	17	from	medium	1628:1633	arg1	pre-growth					1588:1597	pre-growth	1588:1597	pre-growth of Z. mobilis GH3 in sucrose medium	1588:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	3	18	theme	conversion	688:697	arg1	efficiency					674:683	the efficiency	670:683	the efficiency of conversion to useful products	670:716	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	3	19	dep	cellobiose	605:614	arg1	e.g.					599:602	e.g.	599:602	e.g.	599:602	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	0	20	from	growth	100:105	arg1	cellobiose					110:119	cellobiose	110:119	cellobiose	110:119	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	0	21	theme	reprogramming	61:73	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of a glycosyl hydrolase and cellular reprogramming	0:73	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	2	22	theme	Zymomonas	470:478	arg1	mobilis					480:486	Zymomonas mobilis	470:486	Zymomonas mobilis	470:486	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	1	23	theme	renewable	161:169	arg1	biomass					171:177	renewable biomass	161:177	renewable biomass	161:177	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	3	24	link	cellulose-derived	626:642	arg1	oligomers					644:652	longer cellulose-derived oligomers	619:652	longer cellulose-derived oligomers	619:652	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	4	25	theme	Z.	756:757	arg1	mobilis					759:765	Z. mobilis	756:765	Z. mobilis	756:765	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	6	26	from	reversible	1227:1236	arg1	medium					1272:1277	glucose-containing medium	1253:1277	glucose-containing medium	1253:1277	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	4	27	with	hydrolase	834:842	arg1	activity					868:875	β-glucosidase activity	854:875	β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus,	854:941	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	0	28	theme	Zymomonas	82:90	arg1	mobilis					92:98	Zymomonas mobilis	82:98	Zymomonas mobilis growth on cellobiose	82:119	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	1	29	from	biomass	171:177	arg1	chemicals					146:154	chemicals	146:154	chemicals	146:154	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	1	29	from	biomass	171:177	arg1	fuels					136:140	Plant-derived fuels	122:140	Plant-derived fuels	122:140	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	9	30	theme	proteins	1813:1820	arg1	upregulation					1746:1757	upregulation	1746:1757	upregulation	1746:1757	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	6	31	theme	fresh	1378:1382	arg1	medium					1395:1400	fresh cellobiose medium	1378:1400	fresh cellobiose medium	1378:1400	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	0	32	theme	Heterologous	0:11	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of a glycosyl hydrolase and cellular reprogramming	0:73	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	4	33	theme	Z.	792:793	arg1	GH3					803:805	Z. mobilis GH3	792:805	Z. mobilis GH3	792:805	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	4	33	theme	Z.	792:793	arg1	strain					784:789	a strain	782:789	a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus,	782:941	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	0	34	theme	glycosyl	29:36	arg1	hydrolase					38:46	a glycosyl hydrolase	27:46	a glycosyl hydrolase	27:46	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	5	35	theme	extracellular	1179:1191	arg1	release					1193:1199	extracellular release	1179:1199	extracellular release of GH	1179:1205	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	5	36	theme	lag	1051:1053	arg1	phase					1055:1059	a prolonged lag phase	1039:1059	a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH	1039:1205	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	9	37	theme	GHs	1867:1869	arg1	entry					1872:1876	entry	1872:1876	entry of cellobiose into the periplasm	1872:1909	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	9	37	theme	GHs	1867:1869	arg1	release					1837:1843	release	1837:1843	release	1837:1843	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	9	37	theme	GHs	1867:1869	arg1	display					1856:1862	surface display	1848:1862	surface display	1848:1862	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	3	38	theme	useful	702:707	arg1	products					709:716	useful products	702:716	useful products	702:716	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	1	39	link	Plant-derived	122:134	arg1	fuels					136:140	Plant-derived fuels	122:140	Plant-derived fuels	122:140	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	9	40	theme	cellobiose	1881:1890	arg1	entry					1872:1876	entry	1872:1876	entry of cellobiose into the periplasm	1872:1909	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	9	40	theme	cellobiose	1881:1890	arg1	release					1837:1843	release	1837:1843	release	1837:1843	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	9	40	theme	cellobiose	1881:1890	arg1	display					1856:1862	surface display	1848:1862	surface display	1848:1862	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	8	41	theme	mobilis	1605:1611	arg1	GH3					1613:1615	Z. mobilis GH3	1602:1615	Z. mobilis GH3 in sucrose medium	1602:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	3	42	theme	non-metabolizable	563:579	arg1	oligosaccharides					581:596	non-metabolizable oligosaccharides	563:596	non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers)	563:653	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	1	43	theme	carbon	258:263	arg1	balance					265:271	global carbon balance	251:271	global carbon balance	251:271	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	9	44	theme	outer	1790:1794	arg1	proteins					1813:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	2	45	theme	industrial	385:394	arg1	microorganisms					396:409	many industrial microorganisms	380:409	many industrial microorganisms	380:409	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	5	46	theme	increased	1154:1162	arg1	expression					1164:1173	increased expression	1154:1173	increased expression	1154:1173	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	7	47	theme	cellobiose	1493:1502	arg1	%					1488:1488	about 50%	1480:1488	about 50% of cellobiose	1480:1502	After adaptation to cellobiose, our GH-expressing strain was able to convert about 50% of cellobiose to glucose within 24 h and use it for growth and ethanol production.					
32797046	7	47	theme	cellobiose	1493:1502	arg1	cellobiose					1493:1502	cellobiose	1493:1502	cellobiose	1493:1502	After adaptation to cellobiose, our GH-expressing strain was able to convert about 50% of cellobiose to glucose within 24 h and use it for growth and ethanol production.					
32797046	4	48	theme	related	884:890	arg1	species					910:916	a related α-proteobacterial species	882:916	a related α-proteobacterial species	882:916	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	4	48	theme	related	884:890	arg1	crescentus					931:940	Caulobacter crescentus	919:940	Caulobacter crescentus	919:940	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	6	49	theme	genetic	1313:1319	arg1	mutation					1321:1328	genetic mutation	1313:1328	genetic mutation	1313:1328	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	5	50	from	Growth	999:1004	arg1	cellobiose					1009:1018	cellobiose	1009:1018	cellobiose	1009:1018	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	9	51	theme	Proteomic	1675:1683	arg1	analysis					1685:1692	Proteomic analysis	1675:1692	Proteomic analysis of cellobiose- and sucrose-adapted strains	1675:1735	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	8	52	theme	immediate	1643:1651	arg1	growth					1653:1658	immediate growth	1643:1658	immediate growth on cellobiose	1643:1672	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	1	53	theme	significant	184:194	arg1	potential					196:204	significant potential	184:204	significant potential	184:204	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	4	54	theme	β-glucosidase	854:866	arg1	activity					868:875	β-glucosidase activity	854:875	β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus,	854:941	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	10	55	theme	Z.	2092:2093	arg1	mobilis					2095:2101	Z. mobilis	2092:2101	Z. mobilis	2092:2101	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	10	56	theme	key	1929:1931	arg1	findings					1933:1940	two key findings	1925:1940	Our two key findings	1921:1940	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	8	57	from	pre-growth	1588:1597	arg1	medium					1628:1633	sucrose medium	1620:1633	sucrose medium	1620:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	3	58	theme	cellulose-derived	626:642	arg1	oligomers					644:652	longer cellulose-derived oligomers	619:652	longer cellulose-derived oligomers	619:652	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	10	59	theme	sucrase	2128:2134	arg1	production					2136:2145	sucrase production	2128:2145	sucrase production	2128:2145	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	2	60	theme	oligosaccharides	331:346	arg1	coli					434:437	Escherichia coli	422:437	Escherichia coli	422:437	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	60	theme	oligosaccharides	331:346	arg1	cerevisiae					454:463	Saccharomyces cerevisiae	440:463	Saccharomyces cerevisiae	440:463	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	60	theme	oligosaccharides	331:346	arg1	fractions					318:326	significant fractions	306:326	significant fractions	306:326	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	60	theme	oligosaccharides	331:346	arg1	mobilis					480:486	Zymomonas mobilis	470:486	Zymomonas mobilis	470:486	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	9	61	theme	strains	1729:1735	arg1	analysis					1685:1692	Proteomic analysis	1675:1692	Proteomic analysis of cellobiose- and sucrose-adapted strains	1675:1735	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	10	62	theme	Z.	1951:1952	arg1	mobilis					1954:1960	Z. mobilis	1951:1960	Z. mobilis	1951:1960	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	8	63	from	GH3	1613:1615	arg1	medium					1628:1633	sucrose medium	1620:1633	sucrose medium	1620:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	8	64	from	growth	1653:1658	arg1	cellobiose					1663:1672	cellobiose	1663:1672	cellobiose	1663:1672	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	1	65	theme	Plant-derived	122:134	arg1	fuels					136:140	Plant-derived fuels	122:140	Plant-derived fuels	122:140	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	9	66	theme	secretion-	1762:1771	arg1	proteins					1813:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	6	67	theme	glucose-containing	1253:1270	arg1	medium					1272:1277	glucose-containing medium	1253:1277	glucose-containing medium	1253:1277	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	6	68	dep	reversible	1227:1236	arg1	meaning					1280:1286	meaning	1280:1286	meaning	1280:1286	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	6	69	from	medium	1272:1277	arg1	reversible					1227:1236	reversible	1227:1236	reversible	1227:1236	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	2	70	theme	plant	283:287	arg1	biomass					289:295	plant biomass	283:295	plant biomass	283:295	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	9	71	theme	transport-	1774:1783	arg1	proteins					1813:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	0	72	theme	mobilis	92:98	arg1	growth					100:105	Zymomonas mobilis growth	82:105	Zymomonas mobilis growth on cellobiose	82:119	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	4	73	from	species	910:916	arg1	activity					868:875	β-glucosidase activity	854:875	β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus,	854:941	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	9	74	theme	membrane-related	1796:1811	arg1	proteins					1813:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	secretion-, transport-, and outer membrane-related proteins	1762:1820	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	7	75	theme	ethanol	1553:1559	arg1	production					1561:1570	ethanol production	1553:1570	ethanol production	1553:1570	After adaptation to cellobiose, our GH-expressing strain was able to convert about 50% of cellobiose to glucose within 24 h and use it for growth and ethanol production.					
32797046	4	76	theme	mobilis	795:801	arg1	GH3					803:805	Z. mobilis GH3	792:805	Z. mobilis GH3	792:805	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	4	76	theme	mobilis	795:801	arg1	strain					784:789	a strain	782:789	a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus,	782:941	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	10	77	theme	carbon	2014:2019	arg1	source					2021:2026	a sole carbon source	2007:2026	a sole carbon source	2007:2026	Our two key findings are that Z. mobilis can be reprogrammed to grow on cellobiose as a sole carbon source and that this reprogramming is related to a natural response of Z. mobilis to sucrose that promotes sucrase production.					
32797046	5	78	from	changes	1095:1101	arg1	composition					1131:1141	cell composition	1126:1141	cell composition	1126:1141	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	5	78	from	changes	1095:1101	arg1	expression					1111:1120	gene expression	1106:1120	gene expression	1106:1120	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	5	79	theme	cell	1126:1129	arg1	composition					1131:1141	cell composition	1126:1141	cell composition	1126:1141	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	4	80	theme	cellobiose	980:989	arg1	medium					991:996	cellobiose medium	980:996	cellobiose medium	980:996	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	6	81	theme	cellobiose	1384:1393	arg1	medium					1395:1400	fresh cellobiose medium	1378:1400	fresh cellobiose medium	1378:1400	These changes were reversible upon growth in glucose-containing medium, meaning they did not result from genetic mutation but could be retained upon transfer of cells to fresh cellobiose medium.					
32797046	2	82	contain	contains	297:304	arg1	biomass					289:295	plant biomass	283:295	plant biomass	283:295	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	82	contain	contains	297:304	arg2	fractions					318:326	significant fractions	306:326	significant fractions	306:326	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	82	contain	contains	297:304	arg2	cerevisiae					454:463	Saccharomyces cerevisiae	440:463	Saccharomyces cerevisiae	440:463	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	82	contain	contains	297:304	arg2	mobilis					480:486	Zymomonas mobilis	470:486	Zymomonas mobilis	470:486	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	2	82	contain	contains	297:304	arg2	coli					434:437	Escherichia coli	422:437	Escherichia coli	422:437	However, plant biomass contains significant fractions of oligosaccharides that are not usable natively by many industrial microorganisms, including Escherichia coli, Saccharomyces cerevisiae, and Zymomonas mobilis.					
32797046	9	83	theme	surface	1848:1854	arg1	display					1856:1862	surface display	1848:1862	surface display	1848:1862	Proteomic analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid release or surface display of GHs, entry of cellobiose into the periplasm, or both.					
32797046	3	84	theme	enzymatic	512:520	arg1	hydrolysis					522:531	chemical or enzymatic hydrolysis	500:531	chemical or enzymatic hydrolysis	500:531	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	0	85	theme	hydrolase	38:46	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of a glycosyl hydrolase and cellular reprogramming	0:73	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	5	86	theme	prolonged	1041:1049	arg1	phase					1055:1059	a prolonged lag phase	1039:1059	a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH	1039:1205	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	3	87	theme	chemical	500:507	arg1	hydrolysis					522:531	chemical or enzymatic hydrolysis	500:531	chemical or enzymatic hydrolysis	500:531	Even after chemical or enzymatic hydrolysis, some carbohydrate remains as non-metabolizable oligosaccharides (e.g., cellobiose or longer cellulose-derived oligomers), thus reducing the efficiency of conversion to useful products.					
32797046	0	88	theme	cellular	52:59	arg1	reprogramming					61:73	cellular reprogramming	52:73	cellular reprogramming	52:73	Heterologous expression of a glycosyl hydrolase and cellular reprogramming enable Zymomonas mobilis growth on cellobiose.					
32797046	8	89	theme	Z.	1602:1603	arg1	GH3					1613:1615	Z. mobilis GH3	1602:1615	Z. mobilis GH3 in sucrose medium	1602:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	5	90	theme	GH	1204:1205	arg1	expression					1164:1173	increased expression	1154:1173	increased expression	1154:1173	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	5	90	theme	GH	1204:1205	arg1	release					1193:1199	extracellular release	1179:1199	extracellular release of GH	1179:1205	Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and extracellular release of GH.					
32797046	8	91	theme	GH3	1613:1615	arg1	pre-growth					1588:1597	pre-growth	1588:1597	pre-growth of Z. mobilis GH3 in sucrose medium	1588:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	1	92	theme	global	251:256	arg1	balance					265:271	global carbon balance	251:271	global carbon balance	251:271	Plant-derived fuels and chemicals from renewable biomass have significant potential to replace reliance on petroleum and improve global carbon balance.					
32797046	8	93	theme	sucrose	1620:1626	arg1	medium					1628:1633	sucrose medium	1620:1633	sucrose medium	1620:1633	Alternatively, pre-growth of Z. mobilis GH3 in sucrose medium enabled immediate growth on cellobiose.					
32797046	4	94	theme	glycosyl	825:832	arg1	GH					845:846	GH	845:846	GH	845:846	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
32797046	4	94	theme	glycosyl	825:832	arg1	hydrolase					834:842	a glycosyl hydrolase	823:842	a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus,	823:941	To begin to address this problem for Z. mobilis, we engineered a strain (Z. mobilis GH3) that expresses a glycosyl hydrolase (GH) with β-glucosidase activity from a related α-proteobacterial species, Caulobacter crescentus, and subjected it to an adaptation in cellobiose medium.					
34976866	12	0	theme	comprehensive	1680:1692	arg1	understanding					1694:1706	a comprehensive understanding	1678:1706	a comprehensive understanding of the composition and function of the microbial community	1678:1765	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	7	1	theme	intrauterine	905:916	arg1	administration					918:931	intrauterine administration	905:931	intrauterine administration of lipopolysaccharide (LPS) after anesthesia	905:976	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	11	2	theme	metabolites	1483:1493	arg1	concentration					1455:1467	the concentration	1451:1467	the concentration of intestinal metabolites	1451:1493	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	11	3	theme	intestinal	1371:1380	arg1	analysis					1395:1402	intestinal metabolomics analysis	1371:1402	intestinal metabolomics analysis in mice	1371:1410	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	13	4	from	endometritis	1865:1876	arg1	future					1885:1890	future	1885:1890	future	1885:1890	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	5	5	theme	acute	614:618	arg1	endometritis					620:631	acute endometritis	614:631	acute endometritis	614:631	However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown.					
34976866	11	6	theme	primary	1569:1575	arg1	acids					1582:1586	primary bile acids	1569:1586	primary bile acids	1569:1586	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	7	7	theme	female	889:894	arg1	mice					896:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	3	8	theme	diseases	362:369	arg1	progression					347:357	The progression	343:357	The progression of diseases	343:369	The progression of diseases is previously known to change the composition and diversity of intestinal microbiota.					
34976866	12	9	theme	function	1731:1738	arg1	understanding					1694:1706	a comprehensive understanding	1678:1706	a comprehensive understanding of the composition and function of the microbial community	1678:1765	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	5	10	theme	endometritis	620:631	arg1	effects					603:609	the effects	599:609	the effects of acute endometritis on intestinal microbiota and its metabolism	599:675	However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown.					
34976866	2	11	theme	diseases	298:305	arg1	related					316:322	related	316:322	related	316:322	In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota.					
34976866	2	11	theme	diseases	298:305	arg1	occurrence					284:293	the occurrence	280:293	the occurrence of diseases	280:305	In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota.					
34976866	10	12	theme	Christensenellaceae_R-7_group	1308:1336	arg1	ratio					1299:1303	a higher ratio	1290:1303	a higher ratio of Christensenellaceae_R-7_group and Parasutterella	1290:1355	Importantly, the control group exhibited a higher ratio of Christensenellaceae_R-7_group and Parasutterella.					
34976866	9	13	theme	Citrobacter	1169:1179	arg1	abundance					1119:1127	the relative abundance	1106:1127	the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter	1106:1179	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	9	13	theme	Citrobacter	1169:1179	arg1	greater					1197:1203	greater	1197:1203	greater	1197:1203	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	4	14	theme	corresponding	486:498	arg1	changes					500:506	corresponding changes	486:506	corresponding changes in metabolites	486:521	Additionally, it also causes corresponding changes in metabolites, primarily by affecting the physiological processes of microbiota.					
34976866	11	15	theme	acids	1582:1586	arg1	oxidation					1519:1527	biological oxidation	1508:1527	biological oxidation	1508:1527	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	11	15	theme	acids	1582:1586	arg1	metabolism					1537:1546	energy metabolism	1530:1546	energy metabolism	1530:1546	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	11	15	theme	acids	1582:1586	arg1	biosynthesis					1553:1564	biosynthesis	1553:1564	biosynthesis	1553:1564	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	8	16	theme	rRNA	999:1002	arg1	sequencing					1009:1018	16S rRNA gene sequencing	995:1018	16S rRNA gene sequencing	995:1018	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	11	17	theme	intestinal	1472:1481	arg1	metabolites					1483:1493	intestinal metabolites	1472:1493	intestinal metabolites	1472:1493	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	9	18	theme	genus	1093:1097	arg1	level					1099:1103	the genus level	1089:1103	the genus level	1089:1103	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	10	19	theme	higher	1292:1297	arg1	ratio					1299:1303	a higher ratio	1290:1303	a higher ratio of Christensenellaceae_R-7_group and Parasutterella	1290:1355	Importantly, the control group exhibited a higher ratio of Christensenellaceae_R-7_group and Parasutterella.					
34976866	5	20	theme	intestinal	636:645	arg1	microbiota					647:656	intestinal microbiota	636:656	intestinal microbiota	636:656	However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown.					
34976866	7	21	theme	BALB/c	882:887	arg1	mice					896:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	3	22	theme	microbiota	445:454	arg1	composition					405:415	composition	405:415	composition	405:415	The progression of diseases is previously known to change the composition and diversity of intestinal microbiota.					
34976866	3	22	theme	microbiota	445:454	arg1	diversity					421:429	diversity	421:429	diversity	421:429	The progression of diseases is previously known to change the composition and diversity of intestinal microbiota.					
34976866	13	23	theme	acute	1859:1863	arg1	endometritis					1865:1876	acute endometritis	1859:1876	acute endometritis in the future	1859:1890	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	24	from	helpful	1816:1822	arg1	treatment					1846:1854	treatment	1846:1854	treatment	1846:1854	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	24	from	helpful	1816:1822	arg1	prevention					1831:1840	prevention	1831:1840	prevention	1831:1840	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	3	25	dep	composition	405:415	arg1	the					401:403	the	401:403	the	401:403	The progression of diseases is previously known to change the composition and diversity of intestinal microbiota.					
34976866	8	26	theme	liquid	1024:1029	arg1	LC-MS					1063:1067	LC-MS	1063:1067	LC-MS	1063:1067	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	8	26	theme	liquid	1024:1029	arg1	spectrometry					1049:1060	liquid chromatogram-mass spectrometry	1024:1060	liquid chromatogram-mass spectrometry (LC-MS)	1024:1068	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	1	27	theme	acute	192:196	arg1	infections					210:219	both acute and chronic infections	187:219	both acute and chronic infections	187:219	Endometritis is generally caused by bacterial infections, including both acute and chronic infections.					
34976866	0	28	theme	Intestinal	9:18	arg1	Metabolomics					20:31	Combined Intestinal Metabolomics	0:31	Combined Intestinal Metabolomics	0:31	Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice.					
34976866	7	29	theme	SPF	877:879	arg1	mice					896:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	0	30	theme	Combined	0:7	arg1	Metabolomics					20:31	Combined Intestinal Metabolomics	0:31	Combined Intestinal Metabolomics	0:31	Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice.					
34976866	4	31	theme	microbiota	578:587	arg1	processes					565:573	the physiological processes	547:573	the physiological processes of microbiota	547:587	Additionally, it also causes corresponding changes in metabolites, primarily by affecting the physiological processes of microbiota.					
34976866	7	32	theme	specific	853:860	arg1	mice					896:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	3	33	theme	intestinal	434:443	arg1	microbiota					445:454	intestinal microbiota	434:454	intestinal microbiota	434:454	The progression of diseases is previously known to change the composition and diversity of intestinal microbiota.					
34976866	11	34	from	analysis	1395:1402	arg1	mice					1407:1410	mice	1407:1410	mice	1407:1410	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	2	35	theme	past	229:232	arg1	decades					238:244	the past few decades	225:244	the past few decades	225:244	In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota.					
34976866	13	36	dep	prevention	1831:1840	arg1	the					1827:1829	the	1827:1829	the	1827:1829	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	6	37	theme	present	703:709	arg1	study					711:715	the present study	699:715	the present study	699:715	Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites.					
34976866	11	38	theme	bile	1577:1580	arg1	acids					1582:1586	primary bile acids	1569:1586	primary bile acids	1569:1586	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	0	39	from	Lipopolysaccharide	91:108	arg1	Mice					113:116	Mice	113:116	Mice	113:116	Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice.					
34976866	9	40	theme	Klebsiella	1132:1141	arg1	abundance					1119:1127	the relative abundance	1106:1127	the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter	1106:1179	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	9	40	theme	Klebsiella	1132:1141	arg1	greater					1197:1203	greater	1197:1203	greater	1197:1203	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	12	41	theme	composition	1715:1725	arg1	understanding					1694:1706	a comprehensive understanding	1678:1706	a comprehensive understanding of the composition and function of the microbial community	1678:1765	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	9	42	theme	relative	1110:1117	arg1	abundance					1119:1127	the relative abundance	1106:1127	the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter	1106:1179	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	9	42	theme	relative	1110:1117	arg1	greater					1197:1203	greater	1197:1203	greater	1197:1203	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	11	43	theme	biological	1508:1517	arg1	oxidation					1519:1527	biological oxidation	1508:1527	biological oxidation	1508:1527	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	13	44	from	future	1885:1890	arg1	treatment					1846:1854	treatment	1846:1854	treatment	1846:1854	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	44	from	future	1885:1890	arg1	prevention					1831:1840	prevention	1831:1840	prevention	1831:1840	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	9	45	theme	control	1234:1240	arg1	group					1242:1246	the control group	1230:1246	the control group	1230:1246	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	12	46	theme	community	1757:1765	arg1	function					1731:1738	function	1731:1738	function	1731:1738	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	12	46	theme	community	1757:1765	arg1	composition					1715:1725	composition	1715:1725	composition	1715:1725	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	1	47	theme	chronic	202:208	arg1	infections					210:219	both acute and chronic infections	187:219	both acute and chronic infections	187:219	Endometritis is generally caused by bacterial infections, including both acute and chronic infections.					
34976866	0	48	theme	Microbiota	37:46	arg1	Analysis					48:55	Microbiota Analysis	37:55	Microbiota Analysis	37:55	Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice.					
34976866	13	49	theme	study	1801:1805	arg1	findings					1784:1791	the findings	1780:1791	the findings of this study	1780:1805	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	49	theme	study	1801:1805	arg1	helpful					1816:1822	helpful	1816:1822	helpful	1816:1822	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	8	50	theme	chromatogram-mass	1031:1047	arg1	LC-MS					1063:1067	LC-MS	1063:1067	LC-MS	1063:1067	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	8	50	theme	chromatogram-mass	1031:1047	arg1	spectrometry					1049:1060	liquid chromatogram-mass spectrometry	1024:1060	liquid chromatogram-mass spectrometry (LC-MS)	1024:1068	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	12	51	dep	composition	1715:1725	arg1	the					1711:1713	the	1711:1713	the	1711:1713	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	9	52	located	found	1185:1189	arg2	abundance					1119:1127	the relative abundance	1106:1127	the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter	1106:1179	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	9	52	located	found	1185:1189	arg1	level					1099:1103	the genus level	1089:1103	the genus level	1089:1103	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	9	52	located	found	1185:1189	arg2	greater					1197:1203	greater	1197:1203	greater	1197:1203	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	0	53	theme	Acute	61:65	arg1	Endometritis					67:78	Acute Endometritis	61:78	Acute Endometritis	61:78	Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice.					
34976866	7	54	theme	pathogen-free	862:874	arg1	mice					896:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	30 specific pathogen-free (SPF) BALB/c female mice	850:899	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	13	55	from	treatment	1846:1854	arg1	findings					1784:1791	the findings	1780:1791	the findings of this study	1780:1805	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	55	from	treatment	1846:1854	arg1	helpful					1816:1822	helpful	1816:1822	helpful	1816:1822	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	55	from	treatment	1846:1854	arg1	future					1885:1890	future	1885:1890	future	1885:1890	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	2	56	theme	few	234:236	arg1	decades					238:244	the past few decades	225:244	the past few decades	225:244	In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota.					
34976866	9	57	theme	Lachnoclostridium_5	1144:1162	arg1	abundance					1119:1127	the relative abundance	1106:1127	the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter	1106:1179	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	9	57	theme	Lachnoclostridium_5	1144:1162	arg1	greater					1197:1203	greater	1197:1203	greater	1197:1203	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	5	58	from	effects	603:609	arg1	microbiota					647:656	intestinal microbiota	636:656	intestinal microbiota	636:656	However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown.					
34976866	5	58	from	effects	603:609	arg1	metabolism					666:675	its metabolism	662:675	its metabolism	662:675	However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown.					
34976866	11	59	theme	metabolomics	1382:1393	arg1	analysis					1395:1402	intestinal metabolomics analysis	1371:1402	intestinal metabolomics analysis in mice	1371:1410	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	4	60	from	changes	500:506	arg1	metabolites					511:521	metabolites	511:521	metabolites	511:521	Additionally, it also causes corresponding changes in metabolites, primarily by affecting the physiological processes of microbiota.					
34976866	6	61	theme	intestinal	770:779	arg1	microbes					781:788	intestinal microbes	770:788	intestinal microbes	770:788	Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites.					
34976866	13	62	from	prevention	1831:1840	arg1	findings					1784:1791	the findings	1780:1791	the findings of this study	1780:1805	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	62	from	prevention	1831:1840	arg1	helpful					1816:1822	helpful	1816:1822	helpful	1816:1822	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	62	from	prevention	1831:1840	arg1	future					1885:1890	future	1885:1890	future	1885:1890	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	7	63	theme	lipopolysaccharide	936:953	arg1	administration					918:931	intrauterine administration	905:931	intrauterine administration of lipopolysaccharide (LPS) after anesthesia	905:976	Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia.					
34976866	4	64	theme	physiological	551:563	arg1	processes					565:573	the physiological processes	547:573	the physiological processes of microbiota	547:587	Additionally, it also causes corresponding changes in metabolites, primarily by affecting the physiological processes of microbiota.					
34976866	9	65	theme	LPS	1212:1214	arg1	group					1216:1220	the LPS group	1208:1220	the LPS group	1208:1220	At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group.					
34976866	12	66	theme	correlation	1593:1603	arg1	analysis					1605:1612	The correlation analysis	1589:1612	The correlation analysis between microbial diversity and metabolome	1589:1655	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	8	67	theme	16S	995:997	arg1	sequencing					1009:1018	16S rRNA gene sequencing	995:1018	16S rRNA gene sequencing	995:1018	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	8	68	theme	gene	1004:1007	arg1	sequencing					1009:1018	16S rRNA gene sequencing	995:1018	16S rRNA gene sequencing	995:1018	Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed.					
34976866	10	69	theme	Parasutterella	1342:1355	arg1	ratio					1299:1303	a higher ratio	1290:1303	a higher ratio of Christensenellaceae_R-7_group and Parasutterella	1290:1355	Importantly, the control group exhibited a higher ratio of Christensenellaceae_R-7_group and Parasutterella.					
34976866	12	70	theme	microbial	1747:1755	arg1	community					1757:1765	the microbial community	1743:1765	the microbial community	1743:1765	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
34976866	6	71	theme	endometritis	754:765	arg1	effects					737:743	the effects	733:743	the effects of acute endometritis on intestinal microbes and their metabolites	733:810	Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites.					
34976866	2	72	theme	gut	327:329	arg1	microbiota					331:340	gut microbiota	327:340	gut microbiota	327:340	In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota.					
34976866	11	73	theme	energy	1530:1535	arg1	metabolism					1537:1546	energy metabolism	1530:1546	energy metabolism	1530:1546	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	13	74	theme	endometritis	1865:1876	arg1	treatment					1846:1854	treatment	1846:1854	treatment	1846:1854	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	13	74	theme	endometritis	1865:1876	arg1	prevention					1831:1840	prevention	1831:1840	prevention	1831:1840	Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.					
34976866	6	75	theme	acute	748:752	arg1	endometritis					754:765	acute endometritis	748:765	acute endometritis	748:765	Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites.					
34976866	1	76	theme	bacterial	155:163	arg1	infections					165:174	bacterial infections	155:174	bacterial infections	155:174	Endometritis is generally caused by bacterial infections, including both acute and chronic infections.					
34976866	1	76	theme	bacterial	155:163	arg1	infections					210:219	both acute and chronic infections	187:219	both acute and chronic infections	187:219	Endometritis is generally caused by bacterial infections, including both acute and chronic infections.					
34976866	11	77	theme	acute	1424:1428	arg1	endometritis					1430:1441	acute endometritis	1424:1441	acute endometritis	1424:1441	Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids.					
34976866	2	78	theme	accumulated	247:257	arg1	evidence					259:266	accumulated evidence	247:266	accumulated evidence	247:266	In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota.					
34976866	6	79	from	effects	737:743	arg1	microbes					781:788	intestinal microbes	770:788	intestinal microbes	770:788	Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites.					
34976866	6	79	from	effects	737:743	arg1	metabolites					800:810	their metabolites	794:810	their metabolites	794:810	Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites.					
34976866	10	80	theme	control	1266:1272	arg1	group					1274:1278	the control group	1262:1278	the control group	1262:1278	Importantly, the control group exhibited a higher ratio of Christensenellaceae_R-7_group and Parasutterella.					
34976866	12	81	theme	microbial	1622:1630	arg1	diversity					1632:1640	microbial diversity	1622:1640	microbial diversity	1622:1640	The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community.					
32813536	6	0	theme	membrane	1219:1226	arg1	polarization					1239:1250	cellular membrane dielectric polarization	1210:1250	cellular membrane dielectric polarization	1210:1250	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	6	1	theme	cellular	1210:1217	arg1	polarization					1239:1250	cellular membrane dielectric polarization	1210:1250	cellular membrane dielectric polarization	1210:1250	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	4	2	used	used	618:621	arg2	frequencies					601:611	The crossover frequencies	587:611	The crossover frequencies	587:611	The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes.					
32813536	3	3	theme	force	446:450	arg1	spectroscopy					452:463	dielectrophoresis force spectroscopy	428:463	dielectrophoresis force spectroscopy	428:463	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	4	4	theme	capacitance	658:668	arg1	extraction					631:640	the extraction	627:640	the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes	627:744	The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes.					
32813536	4	5	theme	variable	649:656	arg1	capacitance					658:668	the variable capacitance	645:668	the variable capacitance	645:668	The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes.					
32813536	3	6	theme	dielectric	388:397	arg1	polarization					399:410	the dielectric polarization	384:410	the dielectric polarization of a cell using dielectrophoresis force spectroscopy	384:463	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	3	7	theme	sweep	558:562	arg1	rate					564:567	sweep rate	558:567	sweep rate	558:567	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	2	8	theme	polarization	308:319	arg1	characteristics					321:335	the variable dielectric polarization characteristics	284:335	the variable dielectric polarization characteristics of cell membranes	284:353	Herein, the variable dielectric polarization characteristics of cell membranes are reported.					
32813536	5	9	theme	polarization	959:970	arg1	characteristics					972:986	the variable dielectric polarization characteristics	935:986	the variable dielectric polarization characteristics	935:986	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	2	10	theme	dielectric	297:306	arg1	characteristics					321:335	the variable dielectric polarization characteristics	284:335	the variable dielectric polarization characteristics of cell membranes	284:353	Herein, the variable dielectric polarization characteristics of cell membranes are reported.					
32813536	1	11	theme	biological	189:198	arg1	activities					200:209	the biological activities	185:209	the biological activities that sustain cellular life and realize cellular functionalities	185:273	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	0	12	theme	Membrane	9:16	arg1	Characteristic					42:55	Variable Membrane Dielectric Polarization Characteristic	0:55	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells	0:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	0	13	from	Characteristic	42:55	arg1	Cells					76:80	Individual Live Cells	60:80	Individual Live Cells	60:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	3	14	theme	frequency	576:584	arg1	direction					544:552	the direction	540:552	the direction	540:552	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	3	14	theme	frequency	576:584	arg1	rate					564:567	sweep rate	558:567	sweep rate	558:567	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	3	15	theme	crossover	485:493	arg1	frequencies					495:505	different cellular crossover frequencies	466:505	different cellular crossover frequencies	466:505	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	0	16	theme	Variable	0:7	arg1	Characteristic					42:55	Variable Membrane Dielectric Polarization Characteristic	0:55	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells	0:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	1	17	theme	dielectric	104:113	arg1	properties					115:124	the dielectric properties	100:124	the dielectric properties of cell membranes	100:142	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	4	18	theme	cell	731:734	arg1	membranes					736:744	the cell membranes	727:744	the cell membranes	727:744	The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes.					
32813536	0	19	theme	Polarization	29:40	arg1	Characteristic					42:55	Variable Membrane Dielectric Polarization Characteristic	0:55	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells	0:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	1	20	theme	properties	115:124	arg1	Investigation					83:95	Investigation	83:95	Investigation of the dielectric properties of cell membranes	83:142	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	0	21	theme	Dielectric	18:27	arg1	Characteristic					42:55	Variable Membrane Dielectric Polarization Characteristic	0:55	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells	0:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	5	22	theme	variable	765:772	arg1	phenomenon					774:783	this variable phenomenon	760:783	this variable phenomenon	760:783	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	2	23	theme	cell	340:343	arg1	membranes					345:353	cell membranes	340:353	cell membranes	340:353	Herein, the variable dielectric polarization characteristics of cell membranes are reported.					
32813536	1	24	theme	cellular	224:231	arg1	life					233:236	cellular life	224:236	cellular life	224:236	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	3	25	theme	different	466:474	arg1	frequencies					495:505	different cellular crossover frequencies	466:505	different cellular crossover frequencies	466:505	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	5	26	theme	membrane	992:999	arg1	changes					1013:1019	membrane composition changes	992:1019	membrane composition changes	992:1019	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	5	27	theme	variable	939:946	arg1	polarization					959:970	the variable dielectric polarization	935:970	the variable dielectric polarization characteristics	935:986	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	3	28	theme	cellular	476:483	arg1	frequencies					495:505	different cellular crossover frequencies	466:505	different cellular crossover frequencies	466:505	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	4	29	theme	crossover	591:599	arg1	frequencies					601:611	The crossover frequencies	587:611	The crossover frequencies	587:611	The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes.					
32813536	3	30	theme	cell	417:420	arg1	polarization					399:410	the dielectric polarization	384:410	the dielectric polarization of a cell using dielectrophoresis force spectroscopy	384:463	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32813536	4	31	theme	dielectric	696:705	arg1	polarization					707:718	the dielectric polarization	692:718	the dielectric polarization across the cell membranes	692:744	The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes.					
32813536	0	32	theme	Live	71:74	arg1	Cells					76:80	Individual Live Cells	60:80	Individual Live Cells	60:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	5	33	theme	composition	1001:1011	arg1	changes					1013:1019	membrane composition changes	992:1019	membrane composition changes	992:1019	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	6	34	theme	stimuli	1158:1164	arg1	regulation					1135:1144	the regulation	1131:1144	the regulation of external stimuli	1131:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	1	35	theme	cell	129:132	arg1	membranes					134:142	cell membranes	129:142	cell membranes	129:142	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	0	36	theme	Individual	60:69	arg1	Cells					76:80	Individual Live Cells	60:80	Individual Live Cells	60:80	Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells.					
32813536	6	37	theme	powerful	1181:1188	arg1	platform					1190:1197	a powerful platform	1179:1197	a powerful platform to explore cellular membrane dielectric polarization	1179:1250	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	5	38	theme	strong	908:913	arg1	correlation					915:925	a strong correlation	906:925	a strong correlation between the variable dielectric polarization characteristics and membrane composition changes	906:1019	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	1	39	theme	membranes	134:142	arg1	properties					115:124	the dielectric properties	100:124	the dielectric properties of cell membranes	100:142	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	6	40	theme	polarization	1058:1069	arg1	properties					1071:1080	the dielectric polarization properties	1043:1080	the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli	1043:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	6	41	theme	live	1085:1088	arg1	membranes					1097:1105	live cells' membranes	1085:1105	live cells' membranes that can be modified by the regulation of external stimuli	1085:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	2	42	theme	variable	288:295	arg1	characteristics					321:335	the variable dielectric polarization characteristics	284:335	the variable dielectric polarization characteristics of cell membranes	284:353	Herein, the variable dielectric polarization characteristics of cell membranes are reported.					
32813536	2	43	theme	membranes	345:353	arg1	characteristics					321:335	the variable dielectric polarization characteristics	284:335	the variable dielectric polarization characteristics of cell membranes	284:353	Herein, the variable dielectric polarization characteristics of cell membranes are reported.					
32813536	6	44	theme	external	1149:1156	arg1	stimuli					1158:1164	external stimuli	1149:1164	external stimuli	1149:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	5	45	with	cholesterol-depleted	842:861	arg1	methyl-β-cyclodextrin					868:888	methyl-β-cyclodextrin	868:888	methyl-β-cyclodextrin	868:888	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	6	46	theme	dielectric	1228:1237	arg1	polarization					1239:1250	cellular membrane dielectric polarization	1210:1250	cellular membrane dielectric polarization	1210:1250	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	1	47	theme	cellular	250:257	arg1	functionalities					259:273	cellular functionalities	250:273	cellular functionalities	250:273	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	5	48	theme	dielectric	948:957	arg1	polarization					959:970	the variable dielectric polarization	935:970	the variable dielectric polarization characteristics	935:986	In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes.					
32813536	1	49	theme	important	153:161	arg1	role					163:166	an important role	150:166	an important role	150:166	Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities.					
32813536	6	50	mod	modified	1119:1126	arg1	membranes					1097:1105	live cells' membranes	1085:1105	live cells' membranes that can be modified by the regulation of external stimuli	1085:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	6	50	mod	modified	1119:1126	arg3	regulation					1135:1144	the regulation	1131:1144	the regulation of external stimuli	1131:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	6	51	theme	dielectric	1047:1056	arg1	properties					1071:1080	the dielectric polarization properties	1043:1080	the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli	1043:1164	This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.					
32813536	3	52	theme	dielectrophoresis	428:444	arg1	spectroscopy					452:463	dielectrophoresis force spectroscopy	428:463	dielectrophoresis force spectroscopy	428:463	In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency.					
32198443	0	0	theme	Filler	99:104	arg1	Effectiveness					30:42	the Effectiveness	26:42	the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	26:104	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	4	1	theme	scanning	474:481	arg1	microscopy					492:501	scanning electron microscopy	474:501	scanning electron microscopy	474:501	The characteristics of HA and HA-PN fillers were compared using scanning electron microscopy and rheometry.					
32198443	9	2	dep	24	932:933	arg1	to					929:930	to	929:930	to	929:930	The HA and HA-PN fillers were injected into SKH1 hairless mice to determine changes in their volume for up to 24 weeks.					
32198443	5	3	theme	morphological	521:533	arg1	difference					535:544	No morphological difference	518:544	No morphological difference	518:544	No morphological difference was noted between the fillers.					
32198443	8	4	theme	better	764:769	arg1	synthesis					780:788	better collagen synthesis	764:788	better collagen synthesis in human and mouse fibroblasts	764:819	It was also found to stimulate better collagen synthesis in human and mouse fibroblasts.					
32198443	0	5	theme	Dermal	92:97	arg1	Filler					99:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	1	6	theme	used	154:157	arg1	HA					107:108	HA	107:108	HA	107:108	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	1	6	theme	used	154:157	arg1	filler					166:171	the most commonly used dermal filler	136:171	the most commonly used dermal filler	136:171	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	1	7	dep	HA	107:108	arg1	filler					128:133	filler	128:133	filler	128:133	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	1	7	dep	HA	107:108	arg1	acid					122:125	Hyaluronic acid	111:125	Hyaluronic acid	111:125	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	7	8	theme	wound	712:716	arg1	healing					718:724	a wound healing	710:724	a wound healing assay	710:730	The HA-PN filler induced higher cell migration than the HA filler in a wound healing assay.					
32198443	11	9	theme	HA-PN	1101:1105	arg1	fillers					1107:1113	the HA and HA-PN fillers	1090:1113	the HA and HA-PN fillers	1090:1113	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	3	10	dep	efficacies	361:370	arg1	the					357:359	the	357:359	the	357:359	In this study, we compare the efficacies and safety profiles of these fillers.					
32198443	12	11	theme	skin	1354:1357	arg1	regeneration					1359:1370	skin regeneration	1354:1370	skin regeneration	1354:1370	In conclusion, HA-PN complex fillers can stimulate fibroblast growth and facilitate volume growth and skin regeneration.					
32198443	2	12	theme	excellent	271:279	arg1	biocompatibility					281:296	excellent biocompatibility	271:296	excellent biocompatibility	271:296	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	4	13	dep	HA	433:434	arg1	fillers					446:452	fillers	446:452	fillers	446:452	The characteristics of HA and HA-PN fillers were compared using scanning electron microscopy and rheometry.					
32198443	10	14	theme	HA-PN	1030:1034	arg1	filler					1044:1049	the HA-PN complex filler	1026:1049	the HA-PN complex filler	1026:1049	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	8	15	theme	collagen	771:778	arg1	synthesis					780:788	better collagen synthesis	764:788	better collagen synthesis in human and mouse fibroblasts	764:819	It was also found to stimulate better collagen synthesis in human and mouse fibroblasts.					
32198443	11	16	theme	potential	1175:1183	arg1	vanilloid					1185:1193	transient receptor potential vanilloid 4	1156:1195	a lower transient receptor potential vanilloid 4 expression	1148:1206	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	10	17	located	observed	995:1002	arg2	migration					957:965	Increased cell migration	942:965	Increased cell migration	942:965	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	10	17	located	observed	995:1002	arg1	mice					1007:1010	mice	1007:1010	mice injected with the HA-PN complex filler	1007:1049	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	10	17	located	observed	995:1002	arg2	synthesis					980:988	collagen synthesis	971:988	collagen synthesis	971:988	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	1	18	theme	dermal	159:164	arg1	HA					107:108	HA	107:108	HA	107:108	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	1	18	theme	dermal	159:164	arg1	filler					166:171	the most commonly used dermal filler	136:171	the most commonly used dermal filler	136:171	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	11	19	theme	fillers	1107:1113	arg1	durability					1076:1085	durability	1076:1085	durability	1076:1085	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	11	19	theme	fillers	1107:1113	arg1	safety					1065:1070	safety	1065:1070	safety	1065:1070	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	2	20	theme	composite	249:257	arg1	HA-PN					203:207	HA-PN	203:207	HA-PN (Hyaluronic acid-Polynucleotide)	203:240	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	2	20	theme	composite	249:257	arg1	filler					259:264	a new composite filler	243:264	a new composite filler	243:264	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	3	21	theme	fillers	401:407	arg1	efficacies					361:370	efficacies	361:370	efficacies	361:370	In this study, we compare the efficacies and safety profiles of these fillers.					
32198443	3	21	theme	fillers	401:407	arg1	profiles					383:390	safety profiles	376:390	safety profiles	376:390	In this study, we compare the efficacies and safety profiles of these fillers.					
32198443	0	22	theme	Comparative	0:10	arg1	Evaluation					12:21	Comparative Evaluation	0:21	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.	0:105	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	9	23	theme	HA	826:827	arg1	fillers					839:845	The HA and HA-PN fillers	822:845	The HA and HA-PN fillers	822:845	The HA and HA-PN fillers were injected into SKH1 hairless mice to determine changes in their volume for up to 24 weeks.					
32198443	7	24	theme	HA	697:698	arg1	filler					700:705	the HA filler	693:705	the HA filler	693:705	The HA-PN filler induced higher cell migration than the HA filler in a wound healing assay.					
32198443	11	25	theme	receptor	1166:1173	arg1	vanilloid					1185:1193	transient receptor potential vanilloid 4	1156:1195	a lower transient receptor potential vanilloid 4 expression	1148:1206	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	12	26	theme	fibroblast	1303:1312	arg1	growth					1314:1319	fibroblast growth	1303:1319	fibroblast growth	1303:1319	In conclusion, HA-PN complex fillers can stimulate fibroblast growth and facilitate volume growth and skin regeneration.					
32198443	1	27	theme	several	181:187	arg1	effects					194:200	several side effects	181:200	several side effects	181:200	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	9	28	theme	HA-PN	833:837	arg1	fillers					839:845	The HA and HA-PN fillers	822:845	The HA and HA-PN fillers	822:845	The HA and HA-PN fillers were injected into SKH1 hairless mice to determine changes in their volume for up to 24 weeks.					
32198443	4	29	theme	HA	433:434	arg1	characteristics					414:428	The characteristics	410:428	The characteristics of HA and HA-PN fillers	410:452	The characteristics of HA and HA-PN fillers were compared using scanning electron microscopy and rheometry.					
32198443	1	30	theme	Hyaluronic	111:120	arg1	acid					122:125	Hyaluronic acid	111:125	Hyaluronic acid	111:125	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	1	31	theme	side	189:192	arg1	effects					194:200	several side effects	181:200	several side effects	181:200	HA (Hyaluronic acid) filler, the most commonly used dermal filler, causes several side effects.					
32198443	2	32	contain	has	267:269	arg1	HA-PN					203:207	HA-PN	203:207	HA-PN (Hyaluronic acid-Polynucleotide)	203:240	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	2	32	contain	has	267:269	arg1	acid-Polynucleotide					221:239	Hyaluronic acid-Polynucleotide	210:239	Hyaluronic acid-Polynucleotide	210:239	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	2	32	contain	has	267:269	arg2	biocompatibility					281:296	excellent biocompatibility	271:296	excellent biocompatibility	271:296	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	2	32	contain	has	267:269	arg1	filler					259:264	a new composite filler	243:264	a new composite filler	243:264	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	11	33	theme	lower	1150:1154	arg1	expression					1197:1206	a lower transient receptor potential vanilloid 4 expression	1148:1206	a lower transient receptor potential vanilloid 4 expression	1148:1206	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	6	34	theme	viscosity	608:616	arg1	values					633:638	higher viscosity and elasticity values	601:638	higher viscosity and elasticity values	601:638	However, the latter had higher viscosity and elasticity values.					
32198443	0	35	theme	Effectiveness	30:42	arg1	Evaluation					12:21	Comparative Evaluation	0:21	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.	0:105	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	11	36	theme	transient	1156:1164	arg1	vanilloid					1185:1193	transient receptor potential vanilloid 4	1156:1195	a lower transient receptor potential vanilloid 4 expression	1148:1206	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	4	37	theme	HA-PN	440:444	arg1	characteristics					414:428	The characteristics	410:428	The characteristics of HA and HA-PN fillers	410:452	The characteristics of HA and HA-PN fillers were compared using scanning electron microscopy and rheometry.					
32198443	2	38	theme	Hyaluronic	210:219	arg1	HA-PN					203:207	HA-PN	203:207	HA-PN (Hyaluronic acid-Polynucleotide)	203:240	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	2	38	theme	Hyaluronic	210:219	arg1	acid-Polynucleotide					221:239	Hyaluronic acid-Polynucleotide	210:239	Hyaluronic acid-Polynucleotide	210:239	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	3	39	theme	safety	376:381	arg1	profiles					383:390	safety profiles	376:390	safety profiles	376:390	In this study, we compare the efficacies and safety profiles of these fillers.					
32198443	8	40	from	synthesis	780:788	arg1	fibroblasts					809:819	human and mouse fibroblasts	793:819	human and mouse fibroblasts	793:819	It was also found to stimulate better collagen synthesis in human and mouse fibroblasts.					
32198443	0	41	theme	Acid-Polynucleotide	64:82	arg1	Filler					99:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	11	42	theme	vanilloid	1185:1193	arg1	expression					1197:1206	a lower transient receptor potential vanilloid 4 expression	1148:1206	a lower transient receptor potential vanilloid 4 expression	1148:1206	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	0	43	theme	Novel	47:51	arg1	Filler					99:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	10	44	theme	complex	1036:1042	arg1	filler					1044:1049	the HA-PN complex filler	1026:1049	the HA-PN complex filler	1026:1049	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	7	45	theme	higher	666:671	arg1	migration					678:686	higher cell migration	666:686	higher cell migration	666:686	The HA-PN filler induced higher cell migration than the HA filler in a wound healing assay.					
32198443	9	46	from	changes	898:904	arg1	volume					915:920	their volume	909:920	their volume	909:920	The HA and HA-PN fillers were injected into SKH1 hairless mice to determine changes in their volume for up to 24 weeks.					
32198443	10	47	theme	collagen	971:978	arg1	synthesis					980:988	collagen synthesis	971:988	collagen synthesis	971:988	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	8	48	theme	human	793:797	arg1	fibroblasts					809:819	human and mouse fibroblasts	793:819	human and mouse fibroblasts	793:819	It was also found to stimulate better collagen synthesis in human and mouse fibroblasts.					
32198443	12	49	theme	complex	1273:1279	arg1	fillers					1281:1287	HA-PN complex fillers	1267:1287	HA-PN complex fillers	1267:1287	In conclusion, HA-PN complex fillers can stimulate fibroblast growth and facilitate volume growth and skin regeneration.					
32198443	9	50	theme	SKH1	866:869	arg1	mice					880:883	SKH1 hairless mice	866:883	SKH1 hairless mice	866:883	The HA and HA-PN fillers were injected into SKH1 hairless mice to determine changes in their volume for up to 24 weeks.					
32198443	7	51	theme	cell	673:676	arg1	migration					678:686	higher cell migration	666:686	higher cell migration	666:686	The HA-PN filler induced higher cell migration than the HA filler in a wound healing assay.					
32198443	2	52	theme	new	245:247	arg1	HA-PN					203:207	HA-PN	203:207	HA-PN (Hyaluronic acid-Polynucleotide)	203:240	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	2	52	theme	new	245:247	arg1	filler					259:264	a new composite filler	243:264	a new composite filler	243:264	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	6	53	contain	had	597:599	arg1	latter					590:595	latter	590:595	latter	590:595	However, the latter had higher viscosity and elasticity values.					
32198443	6	53	contain	had	597:599	arg2	values					633:638	higher viscosity and elasticity values	601:638	higher viscosity and elasticity values	601:638	However, the latter had higher viscosity and elasticity values.					
32198443	12	54	theme	volume	1336:1341	arg1	growth					1343:1348	volume growth	1336:1348	volume growth	1336:1348	In conclusion, HA-PN complex fillers can stimulate fibroblast growth and facilitate volume growth and skin regeneration.					
32198443	9	55	theme	hairless	871:878	arg1	mice					880:883	SKH1 hairless mice	866:883	SKH1 hairless mice	866:883	The HA and HA-PN fillers were injected into SKH1 hairless mice to determine changes in their volume for up to 24 weeks.					
32198443	10	56	theme	cell	952:955	arg1	migration					957:965	Increased cell migration	942:965	Increased cell migration	942:965	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	7	57	theme	HA-PN	645:649	arg1	filler					651:656	The HA-PN filler	641:656	The HA-PN filler	641:656	The HA-PN filler induced higher cell migration than the HA filler in a wound healing assay.					
32198443	2	58	theme	tissue	310:315	arg1	regeneration					317:328	tissue regeneration	310:328	tissue regeneration	310:328	HA-PN (Hyaluronic acid-Polynucleotide), a new composite filler, has excellent biocompatibility and induces tissue regeneration.					
32198443	8	59	theme	mouse	803:807	arg1	fibroblasts					809:819	human and mouse fibroblasts	793:819	human and mouse fibroblasts	793:819	It was also found to stimulate better collagen synthesis in human and mouse fibroblasts.					
32198443	12	60	theme	HA-PN	1267:1271	arg1	fillers					1281:1287	HA-PN complex fillers	1267:1287	HA-PN complex fillers	1267:1287	In conclusion, HA-PN complex fillers can stimulate fibroblast growth and facilitate volume growth and skin regeneration.					
32198443	0	61	theme	Complex	84:90	arg1	Filler					99:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	6	62	theme	higher	601:606	arg1	values					633:638	higher viscosity and elasticity values	601:638	higher viscosity and elasticity values	601:638	However, the latter had higher viscosity and elasticity values.					
32198443	10	63	theme	Increased	942:950	arg1	migration					957:965	Increased cell migration	942:965	Increased cell migration	942:965	Increased cell migration and collagen synthesis were observed in mice injected with the HA-PN complex filler.					
32198443	11	64	dep	safety	1065:1070	arg1	the					1061:1063	the	1061:1063	the	1061:1063	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	7	65	theme	healing	718:724	arg1	assay					726:730	a wound healing assay	710:730	a wound healing assay	710:730	The HA-PN filler induced higher cell migration than the HA filler in a wound healing assay.					
32198443	0	66	theme	Hyaluronic	53:62	arg1	Filler					99:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler	47:104	Comparative Evaluation of the Effectiveness of Novel Hyaluronic Acid-Polynucleotide Complex Dermal Filler.					
32198443	4	67	theme	electron	483:490	arg1	microscopy					492:501	scanning electron microscopy	474:501	scanning electron microscopy	474:501	The characteristics of HA and HA-PN fillers were compared using scanning electron microscopy and rheometry.					
32198443	11	68	theme	HA	1094:1095	arg1	fillers					1107:1113	the HA and HA-PN fillers	1090:1113	the HA and HA-PN fillers	1090:1113	Although the safety and durability of the HA and HA-PN fillers were similar, the latter induced a lower transient receptor potential vanilloid 4 expression and caused less stimulation upon injection.					
32198443	6	69	theme	elasticity	622:631	arg1	values					633:638	higher viscosity and elasticity values	601:638	higher viscosity and elasticity values	601:638	However, the latter had higher viscosity and elasticity values.					
32985587	4	0	from	day	904:906	arg1	active					894:899	active	894:899	active	894:899	RNA-based analysis showed that E. fredii LP2/20 immobilized in agar was still metabolically active at day 50 after being introduced into soil.					
32985587	2	1	theme	microbial	573:581	arg1	taxa					583:586	specific microbial taxa	564:586	specific microbial taxa	564:586	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	2	from	information	386:396	arg1	activity					423:430	activity	423:430	activity	423:430	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	2	from	information	386:396	arg1	establishment					405:417	establishment	405:417	establishment	405:417	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	6	3	theme	LP2/20	1206:1211	arg1	Inoculation					1181:1191	Inoculation	1181:1191	Inoculation of E. fredii LP2/20 immobilized in agar	1181:1231	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	4	4	theme	E.	833:834	arg1	LP2/20					843:848	E. fredii LP2/20	833:848	E. fredii LP2/20 immobilized in agar	833:868	RNA-based analysis showed that E. fredii LP2/20 immobilized in agar was still metabolically active at day 50 after being introduced into soil.					
32985587	7	5	theme	rRNA	1431:1434	arg1	amplicons					1436:1444	bacterial 16S rRNA amplicons	1417:1444	bacterial 16S rRNA amplicons	1417:1444	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	5	6	dep	conferred	997:1005	arg1	%					1177:1177	22.44%	1172:1177	22.44%	1172:1177	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	1	7	theme	perlite	236:242	arg1	materials					262:270	agar, alginate, and perlite as immobilization materials	216:270	materials	262:270	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	4	8	theme	RNA-based	802:810	arg1	analysis					812:819	RNA-based analysis	802:819	RNA-based analysis	802:819	RNA-based analysis showed that E. fredii LP2/20 immobilized in agar was still metabolically active at day 50 after being introduced into soil.					
32985587	7	9	theme	bacterial	1417:1425	arg1	amplicons					1436:1444	bacterial 16S rRNA amplicons	1417:1444	bacterial 16S rRNA amplicons	1417:1444	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	9	10	theme	delta-Proteobacteria	1815:1834	arg1	number					1805:1810	the number	1801:1810	the number of delta-Proteobacteria	1801:1834	The numbers of alpha- and gamma-Proteobacteria were significantly increased while the number of delta-Proteobacteria was significantly decreased due to E. fredii LP2/20 establishment.					
32985587	9	11	theme	LP2/20	1881:1886	arg1	establishment					1888:1900	E. fredii LP2/20 establishment	1871:1900	E. fredii LP2/20 establishment	1871:1900	The numbers of alpha- and gamma-Proteobacteria were significantly increased while the number of delta-Proteobacteria was significantly decreased due to E. fredii LP2/20 establishment.					
32985587	1	12	theme	former	177:182	arg1	research					184:191	our former research	173:191	our former research	173:191	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	2	13	theme	inoculant	516:524	arg1	conditions					485:494	field conditions	479:494	field conditions	479:494	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	13	theme	inoculant	516:524	arg1	association					597:607	the association	593:607	the association between the inoculant and soil elements merit further studies	593:669	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	13	theme	inoculant	516:524	arg1	effects					501:507	the effects	497:507	the effects of the inoculant on the soil microbial communities and specific microbial taxa	497:586	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	7	14	theme	High-throughput	1371:1385	arg1	sequencing					1403:1412	High-throughput next-generation sequencing	1371:1412	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons	1371:1444	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	0	15	theme	bacterial	110:118	arg1	structure					130:138	bacterial community structure	110:138	bacterial community structure	110:138	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	5	16	theme	dry	1025:1027	arg1	%					1048:1048	up to 89.94%	1037:1048	up to 89.94%	1037:1048	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	16	theme	dry	1025:1027	arg1	weight					1029:1034	the highest plant dry weight	1007:1034	the highest plant dry weight (up to 89.94%)	1007:1049	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	6	17	theme	soil	1268:1271	arg1	matter					1281:1286	soil organic matter	1268:1286	soil organic matter (99.02%)	1268:1295	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	17	theme	soil	1268:1271	arg1	%					1294:1294	99.02%	1289:1294	99.02%	1289:1294	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	10	18	theme	soil	1931:1934	arg1	total					1908:1912	Soil total P, K, and Ca	1903:1925	total	1908:1912	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	18	theme	soil	1931:1934	arg1	K					1917:1917	K	1917:1917	K	1917:1917	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	18	theme	soil	1931:1934	arg1	Ca					1924:1925	Ca	1924:1925	Ca	1924:1925	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	18	theme	soil	1931:1934	arg1	pH					1936:1937	soil pH	1931:1937	soil pH	1931:1937	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	18	theme	soil	1931:1934	arg1	factors					1958:1964	the important factors	1944:1964	the important factors that shaped the soil bacterial community composition	1944:2017	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	9	19	theme	E.	1871:1872	arg1	establishment					1888:1900	E. fredii LP2/20 establishment	1871:1900	E. fredii LP2/20 establishment	1871:1900	The numbers of alpha- and gamma-Proteobacteria were significantly increased while the number of delta-Proteobacteria was significantly decreased due to E. fredii LP2/20 establishment.					
32985587	0	20	theme	structure	130:138	arg1	alteration					96:105	alteration	96:105	alteration of bacterial community structure of Chinese kale planted soil	96:167	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	0	20	theme	structure	130:138	arg1	promotion					82:90	growth promotion	75:90	growth promotion	75:90	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	8	21	theme	total	1679:1683	arg1	richness					1695:1702	total bacterial richness	1679:1702	total bacterial richness	1679:1702	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	2	22	theme	microbial	538:546	arg1	communities					548:558	the soil microbial communities	529:558	the soil microbial communities	529:558	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	3	23	theme	suitable	710:717	arg1	material					719:726	the most suitable material	701:726	the most suitable material that supported the establishment of the inoculant under field conditions	701:799	Here, we found that agar was the most suitable material that supported the establishment of the inoculant under field conditions.					
32985587	3	23	theme	suitable	710:717	arg1	agar					692:695	agar	692:695	agar	692:695	Here, we found that agar was the most suitable material that supported the establishment of the inoculant under field conditions.					
32985587	0	24	theme	Chinese	143:149	arg1	kale					151:154	Chinese kale	143:154	Chinese kale planted soil	143:167	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	10	25	theme	community	1997:2005	arg1	composition					2007:2017	the soil bacterial community composition	1978:2017	the soil bacterial community composition	1978:2017	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	2	26	theme	further	655:661	arg1	studies					663:669	merit further studies	649:669	merit further studies	649:669	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	5	27	theme	highest	1011:1017	arg1	%					1048:1048	up to 89.94%	1037:1048	up to 89.94%	1037:1048	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	27	theme	highest	1011:1017	arg1	weight					1029:1034	the highest plant dry weight	1007:1034	the highest plant dry weight (up to 89.94%)	1007:1049	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	8	28	theme	bacterial	1685:1693	arg1	richness					1695:1702	total bacterial richness	1679:1702	total bacterial richness	1679:1702	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	8	29	theme	fredii	1589:1594	arg1	LP2/20					1596:1601	E. fredii LP2/20	1586:1601	E. fredii LP2/20	1586:1601	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	6	30	theme	total	1298:1302	arg1	N					1304:1304	total N	1298:1304	total N (272.48%)	1298:1314	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	30	theme	total	1298:1302	arg1	%					1313:1313	272.48%	1307:1313	272.48%	1307:1313	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	2	31	theme	plant	460:464	arg1	growth					466:471	plant growth	460:471	plant growth	460:471	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	10	32	theme	soil	1982:1985	arg1	composition					2007:2017	the soil bacterial community composition	1978:2017	the soil bacterial community composition	1978:2017	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	3	33	theme	field	784:788	arg1	conditions					790:799	field conditions	784:799	field conditions	784:799	Here, we found that agar was the most suitable material that supported the establishment of the inoculant under field conditions.					
32985587	5	34	theme	plant	1059:1063	arg1	Mg					1138:1139	Mg	1138:1139	Mg (30.76%)	1138:1148	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	34	theme	plant	1059:1063	arg1	Fe					1151:1152	Fe	1151:1152	Fe (29.85%)	1151:1161	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	34	theme	plant	1059:1063	arg1	Zn					1168:1169	Zn	1168:1169	Zn	1168:1169	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	34	theme	plant	1059:1063	arg1	Ca					1125:1126	total N (9.55%), P (17.94%), K (68.42%), Ca	1084:1126	total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%)	1084:1135	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	34	theme	plant	1059:1063	arg1	elements					1065:1072	all plant elements	1055:1072	all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn	1055:1169	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	2	35	dep	inoculant	621:629	arg1	studies					663:669	merit further studies	649:669	merit further studies	649:669	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	3	36	theme	inoculant	768:776	arg1	establishment					747:759	the establishment	743:759	the establishment of the inoculant	743:776	Here, we found that agar was the most suitable material that supported the establishment of the inoculant under field conditions.					
32985587	1	37	theme	fredii	329:334	arg1	LP2/20					336:341	Ensifer fredii LP2/20	321:341	Ensifer fredii LP2/20	321:341	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	1	37	theme	fredii	329:334	arg1	inoculant					310:318	the inoculant	306:318	the inoculant	306:318	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	8	38	theme	bacterial	1635:1643	arg1	structure					1655:1663	the soil bacterial community structure	1626:1663	the soil bacterial community structure	1626:1663	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	1	39	theme	as	244:245	arg1	materials					262:270	agar, alginate, and perlite as immobilization materials	216:270	materials	262:270	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	7	40	theme	kale	1554:1557	arg1	soil					1565:1568	Chinese kale field soil	1546:1568	Chinese kale field soil	1546:1568	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	0	41	theme	Ensifer	15:21	arg1	strain					30:35	Ensifer fredii strain LP2/20	15:42	Ensifer fredii strain LP2/20	15:42	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	0	42	theme	strain	30:35	arg1	Inoculation					0:10	Inoculation	0:10	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar	0:62	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	7	43	theme	dominant	1528:1535	arg1	phyla					1537:1541	dominant phyla	1528:1541	dominant phyla in Chinese kale field soil	1528:1568	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	7	43	theme	dominant	1528:1535	arg1	Proteobacteria					1462:1475	the Proteobacteria	1458:1475	the Proteobacteria	1458:1475	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	5	44	theme	N	1090:1090	arg1	Ca					1125:1126	total N (9.55%), P (17.94%), K (68.42%), Ca	1084:1126	total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%)	1084:1135	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	44	theme	N	1090:1090	arg1	%					1134:1134	39.77%	1129:1134	39.77%	1129:1134	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	45	dep	89.94	1043:1047	arg1	to					1040:1041	to	1040:1041	to	1040:1041	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	6	46	theme	soil	1243:1246	arg1	matter					1281:1286	soil organic matter	1268:1286	soil organic matter (99.02%)	1268:1295	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	46	theme	soil	1243:1246	arg1	K					1329:1329	K	1329:1329	K (52.74%)	1329:1338	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	46	theme	soil	1243:1246	arg1	Fe					1341:1342	Fe	1341:1342	Fe (51.06%)	1341:1351	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	46	theme	soil	1243:1246	arg1	N					1304:1304	total N	1298:1304	total N (272.48%)	1298:1314	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	46	theme	soil	1243:1246	arg1	P					1317:1317	P	1317:1317	P (31.75%)	1317:1326	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	46	theme	soil	1243:1246	arg1	Zn					1358:1359	Zn	1358:1359	Zn (63.10%)	1358:1368	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	46	theme	soil	1243:1246	arg1	chemicals					1248:1256	soil chemicals	1243:1256	soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%)	1243:1368	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	2	47	from	effects	501:507	arg1	communities					548:558	the soil microbial communities	529:558	the soil microbial communities	529:558	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	47	from	effects	501:507	arg1	taxa					583:586	specific microbial taxa	564:586	specific microbial taxa	564:586	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	1	48	theme	agar	216:219	arg1	alginate					222:229	agar, alginate, and perlite as immobilization materials	216:270	alginate	222:229	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	5	49	theme	fredii	963:968	arg1	LP2/20					970:975	E. fredii LP2/20	960:975	E. fredii LP2/20	960:975	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	7	50	theme	amplicons	1436:1444	arg1	sequencing					1403:1412	High-throughput next-generation sequencing	1371:1412	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons	1371:1444	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	5	51	theme	total	1084:1088	arg1	N					1090:1090	total N	1084:1090	total N (9.55%)	1084:1098	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	51	theme	total	1084:1088	arg1	%					1097:1097	9.55%	1093:1097	9.55%	1093:1097	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	51	theme	total	1084:1088	arg1	P					1101:1101	P	1101:1101	P (17.94%)	1101:1110	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	51	theme	total	1084:1088	arg1	K					1113:1113	K	1113:1113	K (68.42%)	1113:1122	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	2	52	theme	specific	564:571	arg1	taxa					583:586	specific microbial taxa	564:586	specific microbial taxa	564:586	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	7	53	theme	16S	1427:1429	arg1	amplicons					1436:1444	bacterial 16S rRNA amplicons	1417:1444	bacterial 16S rRNA amplicons	1417:1444	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	6	54	theme	fredii	1199:1204	arg1	LP2/20					1206:1211	E. fredii LP2/20	1196:1211	E. fredii LP2/20	1196:1211	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	4	55	theme	fredii	836:841	arg1	LP2/20					843:848	E. fredii LP2/20	833:848	E. fredii LP2/20 immobilized in agar	833:868	RNA-based analysis showed that E. fredii LP2/20 immobilized in agar was still metabolically active at day 50 after being introduced into soil.					
32985587	10	56	theme	important	1948:1956	arg1	total					1908:1912	Soil total P, K, and Ca	1903:1925	total	1908:1912	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	56	theme	important	1948:1956	arg1	K					1917:1917	K	1917:1917	K	1917:1917	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	56	theme	important	1948:1956	arg1	Ca					1924:1925	Ca	1924:1925	Ca	1924:1925	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	56	theme	important	1948:1956	arg1	pH					1936:1937	soil pH	1931:1937	soil pH	1931:1937	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	56	theme	important	1948:1956	arg1	factors					1958:1964	the important factors	1944:1964	the important factors that shaped the soil bacterial community composition	1944:2017	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	7	57	theme	next-generation	1387:1401	arg1	sequencing					1403:1412	High-throughput next-generation sequencing	1371:1412	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons	1371:1444	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	0	58	theme	community	120:128	arg1	structure					130:138	bacterial community structure	110:138	bacterial community structure	110:138	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	6	59	theme	organic	1273:1279	arg1	matter					1281:1286	soil organic matter	1268:1286	soil organic matter (99.02%)	1268:1295	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	6	59	theme	organic	1273:1279	arg1	%					1294:1294	99.02%	1289:1294	99.02%	1289:1294	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	1	60	theme	controlled	349:358	arg1	glasshouse					360:369	a controlled glasshouse	347:369	a controlled glasshouse	347:369	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	9	61	theme	fredii	1874:1879	arg1	establishment					1888:1900	E. fredii LP2/20 establishment	1871:1900	E. fredii LP2/20 establishment	1871:1900	The numbers of alpha- and gamma-Proteobacteria were significantly increased while the number of delta-Proteobacteria was significantly decreased due to E. fredii LP2/20 establishment.					
32985587	0	62	theme	kale	151:154	arg1	alteration					96:105	alteration	96:105	alteration of bacterial community structure of Chinese kale planted soil	96:167	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	0	62	theme	kale	151:154	arg1	promotion					82:90	growth promotion	75:90	growth promotion	75:90	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	2	63	theme	soil	533:536	arg1	communities					548:558	the soil microbial communities	529:558	the soil microbial communities	529:558	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	64	from	conditions	485:494	arg1	communities					548:558	the soil microbial communities	529:558	the soil microbial communities	529:558	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	64	from	conditions	485:494	arg1	taxa					583:586	specific microbial taxa	564:586	specific microbial taxa	564:586	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	65	from	association	597:607	arg1	communities					548:558	the soil microbial communities	529:558	the soil microbial communities	529:558	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	65	from	association	597:607	arg1	taxa					583:586	specific microbial taxa	564:586	specific microbial taxa	564:586	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	8	66	theme	E.	1586:1587	arg1	LP2/20					1596:1601	E. fredii LP2/20	1586:1601	E. fredii LP2/20	1586:1601	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	10	67	theme	bacterial	1987:1995	arg1	composition					2007:2017	the soil bacterial community composition	1978:2017	the soil bacterial community composition	1978:2017	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	8	68	theme	LP2/20	1596:1601	arg1	Inoculation					1571:1581	Inoculation	1571:1581	Inoculation of E. fredii LP2/20	1571:1601	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	5	69	theme	plant	1019:1023	arg1	%					1048:1048	up to 89.94%	1037:1048	up to 89.94%	1037:1048	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	5	69	theme	plant	1019:1023	arg1	weight					1029:1034	the highest plant dry weight	1007:1034	the highest plant dry weight (up to 89.94%)	1007:1049	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	0	70	theme	growth	75:80	arg1	promotion					82:90	growth promotion	75:90	growth promotion	75:90	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	1	71	theme	inoculant	310:318	arg1	survival					294:301	long-term survival	284:301	long-term survival of the inoculant, Ensifer fredii LP2/20,	284:342	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	8	72	theme	soil	1630:1633	arg1	structure					1655:1663	the soil bacterial community structure	1626:1663	the soil bacterial community structure	1626:1663	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	1	73	theme	Ensifer	321:327	arg1	LP2/20					336:341	Ensifer fredii LP2/20	321:341	Ensifer fredii LP2/20	321:341	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	1	73	theme	Ensifer	321:327	arg1	inoculant					310:318	the inoculant	306:318	the inoculant	306:318	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	10	74	dep	total	1908:1912	arg1	P					1914:1914	P	1914:1914	P	1914:1914	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	7	75	from	phyla	1537:1541	arg1	soil					1565:1568	Chinese kale field soil	1546:1568	Chinese kale field soil	1546:1568	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	10	76	theme	Soil	1903:1906	arg1	total					1908:1912	Soil total P, K, and Ca	1903:1925	total	1908:1912	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	76	theme	Soil	1903:1906	arg1	K					1917:1917	K	1917:1917	K	1917:1917	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	76	theme	Soil	1903:1906	arg1	Ca					1924:1925	Ca	1924:1925	Ca	1924:1925	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	76	theme	Soil	1903:1906	arg1	pH					1936:1937	soil pH	1931:1937	soil pH	1931:1937	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	10	76	theme	Soil	1903:1906	arg1	factors					1958:1964	the important factors	1944:1964	the important factors that shaped the soil bacterial community composition	1944:2017	Soil total P, K, and Ca and soil pH were the important factors that shaped the soil bacterial community composition.					
32985587	8	77	theme	community	1645:1653	arg1	structure					1655:1663	the soil bacterial community structure	1626:1663	the soil bacterial community structure	1626:1663	Inoculation of E. fredii LP2/20 significantly affected the soil bacterial community structure by decreasing total bacterial richness and diversity.					
32985587	7	78	theme	field	1559:1563	arg1	soil					1565:1568	Chinese kale field soil	1546:1568	Chinese kale field soil	1546:1568	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	2	79	theme	field	479:483	arg1	conditions					485:494	field conditions	479:494	field conditions	479:494	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	80	theme	merit	649:653	arg1	studies					663:669	merit further studies	649:669	merit further studies	649:669	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	6	81	theme	E.	1196:1197	arg1	LP2/20					1206:1211	E. fredii LP2/20	1196:1211	E. fredii LP2/20	1196:1211	Inoculation of E. fredii LP2/20 immobilized in agar increased soil chemicals including soil organic matter (99.02%), total N (272.48%), P (31.75%), K (52.74%), Fe (51.06%), and Zn (63.10%).					
32985587	7	82	theme	Chinese	1546:1552	arg1	soil					1565:1568	Chinese kale field soil	1546:1568	Chinese kale field soil	1546:1568	High-throughput next-generation sequencing of bacterial 16S rRNA amplicons showed that the Proteobacteria, Acidobacteria, Bacteroidetes, and Firmicutes were dominant phyla in Chinese kale field soil.					
32985587	2	83	theme	soil	635:638	arg1	elements					640:647	soil elements	635:647	soil elements	635:647	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	0	84	theme	fredii	23:28	arg1	strain					30:35	Ensifer fredii strain LP2/20	15:42	Ensifer fredii strain LP2/20	15:42	Inoculation of Ensifer fredii strain LP2/20 immobilized in agar results in growth promotion and alteration of bacterial community structure of Chinese kale planted soil.					
32985587	1	85	theme	immobilization	247:260	arg1	materials					262:270	agar, alginate, and perlite as immobilization materials	216:270	materials	262:270	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	5	86	theme	LP2/20	970:975	arg1	Inoculation					945:955	Inoculation	945:955	Inoculation of E. fredii LP2/20 immobilized in agar	945:995	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
32985587	2	87	theme	inoculant	439:447	arg1	activity					423:430	activity	423:430	activity	423:430	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	2	87	theme	inoculant	439:447	arg1	establishment					405:417	establishment	405:417	establishment	405:417	Therefore the information on the establishment and activity of the inoculant to promote plant growth under field conditions, the effects of the inoculant on the soil microbial communities and specific microbial taxa, and the association between the inoculant and soil elements merit further studies.					
32985587	1	88	theme	long-term	284:292	arg1	survival					294:301	long-term survival	284:301	long-term survival of the inoculant, Ensifer fredii LP2/20,	284:342	In our former research, we succeeded in using agar, alginate, and perlite as immobilization materials to maintain long-term survival of the inoculant, Ensifer fredii LP2/20, in a controlled glasshouse.					
32985587	9	89	theme	alpha-	1734:1739	arg1	numbers					1723:1729	The numbers	1719:1729	The numbers of alpha- and gamma-Proteobacteria	1719:1764	The numbers of alpha- and gamma-Proteobacteria were significantly increased while the number of delta-Proteobacteria was significantly decreased due to E. fredii LP2/20 establishment.					
32985587	9	90	theme	gamma-Proteobacteria	1745:1764	arg1	numbers					1723:1729	The numbers	1719:1729	The numbers of alpha- and gamma-Proteobacteria	1719:1764	The numbers of alpha- and gamma-Proteobacteria were significantly increased while the number of delta-Proteobacteria was significantly decreased due to E. fredii LP2/20 establishment.					
32985587	5	91	theme	E.	960:961	arg1	LP2/20					970:975	E. fredii LP2/20	960:975	E. fredii LP2/20	960:975	Inoculation of E. fredii LP2/20 immobilized in agar conferred the highest plant dry weight (up to 89.94%) and all plant elements including total N (9.55%), P (17.94%), K (68.42%), Ca (39.77%), Mg (30.76%), Fe (29.85%), and Zn (22.44%).					
31969191	7	0	theme	genomics	1490:1497	arg1	approach					1499:1506	CONCLUSIONS Our single-cell genomics approach	1462:1506	CONCLUSIONS Our single-cell genomics approach	1462:1506	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	4	1	theme	SAG-gel	890:896	arg1	technique					873:881	our massively parallel single-cell genome sequencing technique	820:881	our massively parallel single-cell genome sequencing technique	820:881	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	1	theme	SAG-gel	890:896	arg1	platform					898:905	named SAG-gel platform	884:905	named SAG-gel platform	884:905	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	3	2	theme	microbiome	687:696	arg1	composition					698:708	the mouse fecal microbiome composition	671:708	the mouse fecal microbiome composition	671:708	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	1	3	from	changes	329:335	arg1	microbiota					344:353	the microbiota	340:353	the microbiota	340:353	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	6	4	theme	succinate	1440:1448	arg1	production					1450:1459	succinate production	1440:1459	succinate production	1440:1459	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	9	5	theme	uncultured	1924:1933	arg1	bacteria					1935:1942	uncultured bacteria	1924:1942	uncultured bacteria with specific functions in the microbiota	1924:1984	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	8	6	theme	dietary	1717:1723	arg1	fiber					1725:1729	dietary fiber	1717:1729	dietary fiber	1717:1729	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	2	7	theme	gut	624:626	arg1	microbiota					628:637	the mouse gut microbiota	614:637	the mouse gut microbiota	614:637	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	8	8	from	production	1785:1794	arg1	environment					1814:1824	the intestinal environment	1799:1824	the intestinal environment based on the fibers ingested	1799:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	0	9	theme	Single-cell	0:10	arg1	genomics					12:19	Single-cell genomics	0:19	Single-cell genomics of uncultured bacteria	0:42	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	4	10	theme	named	884:888	arg1	technique					873:881	our massively parallel single-cell genome sequencing technique	820:881	our massively parallel single-cell genome sequencing technique	820:881	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	10	theme	named	884:888	arg1	platform					898:905	named SAG-gel platform	884:905	named SAG-gel platform	884:905	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	1	11	theme	current	191:197	arg1	strategies					207:216	current genomic strategies	191:216	current genomic strategies for uncultured bacteria	191:240	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	6	12	theme	specific	1408:1415	arg1	pathways					1427:1434	their specific metabolic pathways	1402:1434	their specific metabolic pathways	1402:1434	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	8	13	theme	fatty	1774:1778	arg1	acid					1780:1783	short-chain fatty acid	1762:1783	short-chain fatty acid production in the intestinal environment based on the fibers ingested	1762:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	3	14	theme	abundant	771:778	arg1	succinate					780:788	abundant succinate	771:788	abundant succinate	771:788	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	1	15	theme	gut	121:123	arg1	microbiota					125:134	The gut microbiota	117:134	The gut microbiota	117:134	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	7	16	theme	intestinal	1605:1614	arg1	microbiome					1616:1625	the intestinal microbiome	1601:1625	the intestinal microbiome	1601:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	4	17	theme	single-amplified	924:939	arg1	SAGs					950:953	SAGs	950:953	SAGs	950:953	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	17	theme	single-amplified	924:939	arg1	genomes					941:947	346 single-amplified genomes	920:947	346 single-amplified genomes (SAGs)	920:954	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	9	18	theme	human	2020:2024	arg1	health					2037:2042	human and animal health	2020:2042	human and animal health	2020:2042	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	3	19	theme	mouse	797:801	arg1	intestine					803:811	the mouse intestine	793:811	the mouse intestine	793:811	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	1	20	theme	dramatic	145:152	arg1	effects					154:160	dramatic effects	145:160	dramatic effects	145:160	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	7	21	theme	uncultured	1578:1587	arg1	bacteria					1589:1596	uncultured bacteria	1578:1596	uncultured bacteria in the intestinal microbiome	1578:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	4	22	theme	dietary	997:1003	arg1	supplementation					1012:1026	dietary inulin supplementation	997:1026	dietary inulin supplementation	997:1026	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	9	23	theme	animal	2030:2035	arg1	health					2037:2042	human and animal health	2020:2042	human and animal health	2020:2042	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	5	24	theme	draft	1223:1227	arg1	genomes					1229:1235	draft genomes	1223:1235	draft genomes	1223:1235	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	6	25	dep	inulin-responders	1308:1324	arg1	Bacteroides					1327:1337	the dominant inulin-responders, Bacteroides spp.	1295:1342	Bacteroides	1327:1337	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	6	25	dep	inulin-responders	1308:1324	arg1	spp.					1339:1342	the dominant inulin-responders, Bacteroides spp.	1295:1342	spp.	1339:1342	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	4	26	theme	mouse	961:965	arg1	microbes					971:978	mouse gut microbes	961:978	mouse gut microbes	961:978	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	0	27	theme	gut	90:92	arg1	microbiota					94:103	the mouse gut microbiota	80:103	the mouse gut microbiota	80:103	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	2	28	theme	reference	513:521	arg1	genomes					523:529	reference genomes	513:529	reference genomes	513:529	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	2	29	theme	novel	385:389	arg1	technique					422:430	a novel single-cell genomic sequencing technique	383:430	a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes	383:529	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	6	30	theme	inulin-responders	1308:1324	arg1	genomes					1284:1290	the genomes	1280:1290	the genomes of the dominant inulin-responders, Bacteroides spp.	1280:1342	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	2	31	theme	metabolic	450:458	arg1	responders					460:469	metabolic responders	450:469	metabolic responders	450:469	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	3	32	from	production	757:766	arg1	intestine					803:811	the mouse intestine	793:811	the mouse intestine	793:811	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	6	33	theme	metabolic	1417:1425	arg1	pathways					1427:1434	their specific metabolic pathways	1402:1434	their specific metabolic pathways	1402:1434	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	4	34	theme	sequencing	862:871	arg1	technique					873:881	our massively parallel single-cell genome sequencing technique	820:881	our massively parallel single-cell genome sequencing technique	820:881	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	34	theme	sequencing	862:871	arg1	platform					898:905	named SAG-gel platform	884:905	named SAG-gel platform	884:905	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	7	35	theme	SAGs	1538:1541	arg1	SAGs					1538:1541	SAGs	1538:1541	SAGs	1538:1541	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	7	35	theme	SAGs	1538:1541	arg1	amount					1528:1533	a massive amount	1518:1533	a massive amount of SAGs	1518:1541	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	2	36	theme	species	478:484	arg1	level					486:490	the species level	474:490	the species level	474:490	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	2	37	theme	genomic	403:409	arg1	technique					422:430	a novel single-cell genomic sequencing technique	383:430	a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes	383:529	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	0	38	theme	bacteria	35:42	arg1	genomics					12:19	Single-cell genomics	0:19	Single-cell genomics of uncultured bacteria	0:42	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	7	39	from	analysis	1566:1573	arg1	microbiome					1616:1625	the intestinal microbiome	1601:1625	the intestinal microbiome	1601:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	4	40	theme	single-cell	843:853	arg1	technique					873:881	our massively parallel single-cell genome sequencing technique	820:881	our massively parallel single-cell genome sequencing technique	820:881	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	40	theme	single-cell	843:853	arg1	platform					898:905	named SAG-gel platform	884:905	named SAG-gel platform	884:905	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	0	41	theme	dietary	52:58	arg1	responders					66:75	dietary fiber responders	52:75	dietary fiber responders	52:75	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	1	42	theme	metabolic	319:327	arg1	changes					329:335	metabolic changes	319:335	metabolic changes in the microbiota	319:353	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	4	43	theme	genome	855:860	arg1	technique					873:881	our massively parallel single-cell genome sequencing technique	820:881	our massively parallel single-cell genome sequencing technique	820:881	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	43	theme	genome	855:860	arg1	platform					898:905	named SAG-gel platform	884:905	named SAG-gel platform	884:905	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	3	44	theme	fecal	681:685	arg1	composition					698:708	the mouse fecal microbiome composition	671:708	the mouse fecal microbiome composition	671:708	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	9	45	theme	in-depth	1881:1888	arg1	isolation					1890:1898	in-depth isolation	1881:1898	in-depth isolation	1881:1898	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	3	46	dep	RESULTS	640:646	arg1	changed					663:669	changed	663:669	changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine	663:811	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	6	47	theme	utilization	1381:1391	arg1	loci					1393:1396	their polysaccharide utilization loci	1360:1396	their polysaccharide utilization loci	1360:1396	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	7	48	theme	single-cell	1478:1488	arg1	approach					1499:1506	CONCLUSIONS Our single-cell genomics approach	1462:1506	CONCLUSIONS Our single-cell genomics approach	1462:1506	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	1	49	theme	several	247:253	arg1	limitations					255:265	several limitations	247:265	several limitations that hinder their ability to identify responders to metabolic changes in the microbiota	247:353	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	7	50	theme	CONCLUSIONS	1462:1472	arg1	approach					1499:1506	CONCLUSIONS Our single-cell genomics approach	1462:1506	CONCLUSIONS Our single-cell genomics approach	1462:1506	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	2	51	theme	inulin-based	593:604	arg1	diet					606:609	an inulin-based diet	590:609	an inulin-based diet in the mouse gut microbiota	590:637	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	8	52	theme	bacterial	1691:1699	arg1	fermentation					1701:1712	the bacterial fermentation	1687:1712	the bacterial fermentation of dietary fiber	1687:1729	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	5	53	theme	SAGs	1178:1181	arg1	strains					1167:1173	31 different strains	1154:1173	31 different strains of SAGs	1154:1181	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	5	53	theme	SAGs	1178:1181	arg1	high-					1198:1202	high-	1198:1202	high-	1198:1202	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	5	53	theme	SAGs	1178:1181	arg1	medium-quality					1208:1221	medium-quality	1208:1221	medium-quality	1208:1221	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	9	54	theme	bacteria	1935:1942	arg1	characterization					1904:1919	characterization	1904:1919	characterization	1904:1919	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	9	54	theme	bacteria	1935:1942	arg1	isolation					1890:1898	in-depth isolation	1881:1898	in-depth isolation	1881:1898	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	1	55	theme	host	165:168	arg1	metabolism					170:179	host metabolism	165:179	host metabolism	165:179	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	6	56	theme	polysaccharide	1366:1379	arg1	loci					1393:1396	their polysaccharide utilization loci	1360:1396	their polysaccharide utilization loci	1360:1396	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	2	57	theme	mouse	618:622	arg1	microbiota					628:637	the mouse gut microbiota	614:637	the mouse gut microbiota	614:637	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	9	58	theme	specific	1949:1956	arg1	functions					1958:1966	specific functions	1949:1966	specific functions in the microbiota	1949:1984	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	1	59	contain	have	140:143	arg1	microbiota					125:134	The gut microbiota	117:134	The gut microbiota	117:134	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	1	59	contain	have	140:143	arg2	effects					154:160	dramatic effects	145:160	dramatic effects	145:160	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	8	60	theme	fiber	1725:1729	arg1	outcomes					1745:1752	metabolic outcomes	1735:1752	metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested	1735:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	8	60	theme	fiber	1725:1729	arg1	production					1785:1794	short-chain fatty acid production	1762:1794	short-chain fatty acid production in the intestinal environment based on the fibers ingested	1762:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	8	60	theme	fiber	1725:1729	arg1	fermentation					1701:1712	the bacterial fermentation	1687:1712	the bacterial fermentation of dietary fiber	1687:1729	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	6	61	theme	dominant	1299:1306	arg1	inulin-responders					1308:1324	the dominant inulin-responders	1295:1324	the dominant inulin-responders	1295:1324	From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production.					
31969191	8	62	theme	metabolic	1735:1743	arg1	outcomes					1745:1752	metabolic outcomes	1735:1752	metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested	1735:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	8	62	theme	metabolic	1735:1743	arg1	production					1785:1794	short-chain fatty acid production	1762:1794	short-chain fatty acid production in the intestinal environment based on the fibers ingested	1762:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	5	63	dep	high-	1198:1202	arg1	genomes					1229:1235	draft genomes	1223:1235	draft genomes	1223:1235	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	1	64	contain	have	242:245	arg2	limitations					255:265	several limitations	247:265	several limitations that hinder their ability to identify responders to metabolic changes in the microbiota	247:353	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	1	64	contain	have	242:245	arg1	strategies					207:216	current genomic strategies	191:216	current genomic strategies for uncultured bacteria	191:240	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	1	65	theme	genomic	199:205	arg1	strategies					207:216	current genomic strategies	191:216	current genomic strategies for uncultured bacteria	191:240	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	8	66	theme	short-chain	1762:1772	arg1	acid					1780:1783	short-chain fatty acid	1762:1783	short-chain fatty acid production in the intestinal environment based on the fibers ingested	1762:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	2	67	theme	bacterial	566:574	arg1	responders					576:585	bacterial responders	566:585	bacterial responders to an inulin-based diet in the mouse gut microbiota	566:637	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	3	68	theme	succinate	780:788	arg1	production					757:766	the production	753:766	the production of abundant succinate in the mouse intestine	753:811	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	8	69	theme	acid	1780:1783	arg1	production					1785:1794	short-chain fatty acid production	1762:1794	short-chain fatty acid production in the intestinal environment based on the fibers ingested	1762:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	7	70	theme	bacteria	1589:1596	arg1	analysis					1566:1573	a functional analysis	1553:1573	a functional analysis of uncultured bacteria in the intestinal microbiome	1553:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	10	71	dep	abstract	2051:2058	arg1	Video					2045:2049	Video	2045:2049	Video	2045:2049	Video abstract.					
31969191	9	72	from	functions	1958:1966	arg1	microbiota					1975:1984	the microbiota	1971:1984	the microbiota	1971:1984	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	1	73	dep	BACKGROUND	106:115	arg1	have					140:143	have	140:143	can have dramatic effects on host metabolism	136:179	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
31969191	0	74	theme	mouse	84:88	arg1	microbiota					94:103	the mouse gut microbiota	80:103	the mouse gut microbiota	80:103	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	8	75	theme	intestinal	1803:1812	arg1	environment					1814:1824	the intestinal environment	1799:1824	the intestinal environment based on the fibers ingested	1799:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	7	76	theme	functional	1555:1564	arg1	analysis					1566:1573	a functional analysis	1553:1573	a functional analysis of uncultured bacteria in the intestinal microbiome	1553:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	7	77	from	microbiome	1616:1625	arg1	analysis					1566:1573	a functional analysis	1553:1573	a functional analysis of uncultured bacteria in the intestinal microbiome	1553:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	9	78	dep	isolation	1890:1898	arg1	the					1877:1879	the	1877:1879	the	1877:1879	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	4	79	theme	gut	967:969	arg1	microbes					971:978	mouse gut microbes	961:978	mouse gut microbes	961:978	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	0	80	theme	uncultured	24:33	arg1	bacteria					35:42	uncultured bacteria	24:42	uncultured bacteria	24:42	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	2	81	from	diet	606:609	arg1	microbiota					628:637	the mouse gut microbiota	614:637	the mouse gut microbiota	614:637	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	2	82	theme	sequencing	411:420	arg1	technique					422:430	a novel single-cell genomic sequencing technique	383:430	a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes	383:529	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	5	83	theme	different	1157:1165	arg1	strains					1167:1173	31 different strains	1154:1173	31 different strains of SAGs	1154:1181	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	5	83	theme	different	1157:1165	arg1	high-					1198:1202	high-	1198:1202	high-	1198:1202	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	5	83	theme	different	1157:1165	arg1	medium-quality					1208:1221	medium-quality	1208:1221	medium-quality	1208:1221	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	5	84	theme	quality	1035:1041	arg1	control					1043:1049	quality control	1035:1049	quality control	1035:1049	After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes.					
31969191	4	85	theme	inulin	1005:1010	arg1	supplementation					1012:1026	dietary inulin supplementation	997:1026	dietary inulin supplementation	997:1026	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	2	86	theme	single-cell	391:401	arg1	technique					422:430	a novel single-cell genomic sequencing technique	383:430	a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes	383:529	In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.					
31969191	0	87	theme	fiber	60:64	arg1	responders					66:75	dietary fiber responders	52:75	dietary fiber responders	52:75	Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.					
31969191	3	88	theme	mouse	675:679	arg1	composition					698:708	the mouse fecal microbiome composition	671:708	the mouse fecal microbiome composition	671:708	RESULTS Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine.					
31969191	4	89	theme	parallel	834:841	arg1	technique					873:881	our massively parallel single-cell genome sequencing technique	820:881	our massively parallel single-cell genome sequencing technique	820:881	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	4	89	theme	parallel	834:841	arg1	platform					898:905	named SAG-gel platform	884:905	named SAG-gel platform	884:905	Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation.					
31969191	8	90	theme	metabolic	1656:1664	arg1	lineages					1666:1673	metabolic lineages	1656:1673	metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested	1656:1853	This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested.					
31969191	7	91	from	bacteria	1589:1596	arg1	microbiome					1616:1625	the intestinal microbiome	1601:1625	the intestinal microbiome	1601:1625	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	7	92	theme	massive	1520:1526	arg1	SAGs					1538:1541	SAGs	1538:1541	SAGs	1538:1541	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	7	92	theme	massive	1520:1526	arg1	amount					1528:1533	a massive amount	1518:1533	a massive amount of SAGs	1518:1541	CONCLUSIONS Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome.					
31969191	9	93	with	bacteria	1935:1942	arg1	functions					1958:1966	specific functions	1949:1966	specific functions in the microbiota	1949:1984	The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health.					
31969191	1	94	theme	uncultured	222:231	arg1	bacteria					233:240	uncultured bacteria	222:240	uncultured bacteria	222:240	BACKGROUND The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota.					
33538807	13	0	theme	bacterial	1313:1321	arg1	metabolism					1323:1332	bacterial metabolism	1313:1332	bacterial metabolism in the neonate gut	1313:1351	The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.					
33538807	6	1	theme	urea	634:637	arg1	presence					622:629	the presence	618:629	the presence of urea	618:637	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	13	2	theme	neonate	1341:1347	arg1	gut					1349:1351	the neonate gut	1337:1351	the neonate gut	1337:1351	The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.					
33538807	0	3	theme	Bifidobacterium	58:72	arg1	infantis					74:81	urease positive Bifidobacterium infantis	42:81	urease positive Bifidobacterium infantis	42:81	Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.					
33538807	9	4	theme	metagenome	815:824	arg1	data					826:829	metagenome data	815:829	metagenome data	815:829	To the same end, metagenome data were mined for urease-related genes.					
33538807	7	5	theme	proteins	740:747	arg1	expression					683:692	the expression	679:692	the expression of both urease protein subunits and accessory proteins of B. longum subsp	679:766	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	6	6	theme	nitrogen	543:550	arg1	urea					518:521	urea	518:521	urea	518:521	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	6	6	theme	nitrogen	543:550	arg1	source					533:538	a main source	526:538	a main source of nitrogen for growth in synthetic medium	526:581	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	11	7	theme	urease	1055:1060	arg1	alignments					1076:1085	urease subunit alpha alignments	1055:1085	urease subunit alpha alignments	1055:1085	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	11	8	theme	alignments	1076:1085	arg1	alignments					1076:1085	urease subunit alpha alignments	1055:1085	urease subunit alpha alignments	1055:1085	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	11	8	theme	alignments	1076:1085	arg1	106					1048:1050	106	1048:1050	106	1048:1050	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	7	9	dep	B.	752:753	arg1	longum					755:760	longum	755:760	longum	755:760	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	3	10	theme	early	342:346	arg1	microbiome					353:362	this early life microbiome	337:362	this early life microbiome	337:362	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	4	11	theme	nitrogen	442:449	arg1	part					418:421	a large part	410:421	a large part of the non-protein nitrogen (NPN)	410:455	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	4	12	theme	human	385:389	arg1	milk					391:394	human milk	385:394	human milk	385:394	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	7	13	theme	accessory	730:738	arg1	proteins					740:747	accessory proteins	730:747	accessory proteins	730:747	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	4	14	theme	non-protein	430:440	arg1	NPN					452:454	NPN	452:454	NPN	452:454	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	4	14	theme	non-protein	430:440	arg1	nitrogen					442:449	the non-protein nitrogen	426:449	the non-protein nitrogen (NPN)	426:455	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	4	15	from	abundant	373:380	arg1	milk					391:394	human milk	385:394	human milk	385:394	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	12	16	theme	infant	1194:1199	arg1	intestine					1201:1209	the infant intestine	1190:1209	the infant intestine	1190:1209	These experiments show how an important gut commensal that colonizes the infant intestine can metabolize urea.					
33538807	11	17	theme	subunit	1062:1068	arg1	alignments					1076:1085	urease subunit alpha alignments	1055:1085	urease subunit alpha alignments	1055:1085	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	13	18	from	metabolism	1323:1332	arg1	gut					1349:1351	the neonate gut	1337:1351	the neonate gut	1337:1351	The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.					
33538807	6	19	theme	main	528:531	arg1	urea					518:521	urea	518:521	urea	518:521	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	6	19	theme	main	528:531	arg1	source					533:538	a main source	526:538	a main source of nitrogen for growth in synthetic medium	526:581	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	10	20	theme	2.3-fold	993:1000	arg1	increase					1002:1009	2.3-fold increase	993:1009	51.4:22.1; 2.3-fold increase	982:1009	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	1	21	from	microbiome	129:138	arg1	infants					143:149	infants	143:149	infants	143:149	Human milk stimulates a health-promoting gut microbiome in infants.					
33538807	7	22	theme	subsp	762:766	arg1	subunits					717:724	urease protein subunits	702:724	urease protein subunits	702:724	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	7	22	theme	subsp	762:766	arg1	proteins					740:747	accessory proteins	730:747	accessory proteins	730:747	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	0	23	theme	Breast	0:5	arg1	milk					7:10	Breast milk	0:10	Breast milk	0:10	Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.					
33538807	7	24	theme	subunits	717:724	arg1	expression					683:692	the expression	679:692	the expression of both urease protein subunits and accessory proteins of B. longum subsp	679:766	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	1	25	theme	Human	84:88	arg1	milk					90:93	Human milk	84:93	Human milk	84:93	Human milk stimulates a health-promoting gut microbiome in infants.					
33538807	10	26	theme	more	930:933	arg1	genes					950:954	more urease-related genes	930:954	more urease-related genes	930:954	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	3	27	theme	life	348:351	arg1	microbiome					353:362	this early life microbiome	337:362	this early life microbiome	337:362	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	10	28	theme	breastfed	890:898	arg1	microbiome					909:918	the breastfed infant's microbiome	886:918	the breastfed infant's microbiome	886:918	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	3	29	theme	milk	263:266	arg1	sources					277:283	Human milk nitrogen sources	257:283	Human milk nitrogen sources such as urea	257:296	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	3	29	theme	milk	263:266	arg1	urea					293:296	urea	293:296	urea	293:296	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	7	30	theme	B.	752:753	arg1	subsp					762:766	B. longum subsp	752:766	B. longum subsp	752:766	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	3	31	theme	microbiome	353:362	arg1	composition					322:332	the composition	318:332	the composition of this early life microbiome	318:362	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	7	32	theme	urease	702:707	arg1	subunits					717:724	urease protein subunits	702:724	urease protein subunits	702:724	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	3	33	theme	nitrogen	268:275	arg1	sources					277:283	Human milk nitrogen sources	257:283	Human milk nitrogen sources such as urea	257:296	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	3	33	theme	nitrogen	268:275	arg1	urea					293:296	urea	293:296	urea	293:296	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	10	34	theme	urease-related	935:948	arg1	genes					950:954	more urease-related genes	930:954	more urease-related genes	930:954	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	6	35	theme	enzyme	587:592	arg1	activity					594:601	enzyme activity	587:601	enzyme activity	587:601	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	2	36	from	milk	251:254	arg1	nitrogen					230:237	its required nitrogen	217:237	its required nitrogen	217:237	However, it is unclear how the microbiota salvages and processes its required nitrogen from breast milk.					
33538807	12	37	theme	gut	1161:1163	arg1	commensal					1165:1173	an important gut commensal	1148:1173	an important gut commensal that colonizes the infant intestine	1148:1209	These experiments show how an important gut commensal that colonizes the infant intestine can metabolize urea.					
33538807	0	38	theme	nitrogen	22:29	arg1	source					31:36	a nitrogen source	20:36	a nitrogen source for urease positive Bifidobacterium infantis	20:81	Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.					
33538807	7	39	theme	protein	709:715	arg1	subunits					717:724	urease protein subunits	702:724	urease protein subunits	702:724	We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp.					
33538807	4	40	theme	large	412:416	arg1	part					418:421	a large part	410:421	a large part of the non-protein nitrogen (NPN)	410:455	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	2	41	theme	required	221:228	arg1	nitrogen					230:237	its required nitrogen	217:237	its required nitrogen	217:237	However, it is unclear how the microbiota salvages and processes its required nitrogen from breast milk.					
33538807	5	42	dep	B.	472:473	arg1	longum					475:480	longum	475:480	longum	475:480	We found that B. longum subsp.					
33538807	3	43	theme	Human	257:261	arg1	sources					277:283	Human milk nitrogen sources	257:283	Human milk nitrogen sources such as urea	257:296	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	3	43	theme	Human	257:261	arg1	urea					293:296	urea	293:296	urea	293:296	Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome.					
33538807	8	44	dep	infantis	769:776	arg1	proteomics					786:795	proteomics	786:795	proteomics	786:795	infantis through proteomics.					
33538807	10	45	dep	infants	973:979	arg1	51.4:22.1					982:990	51.4:22.1	982:990	51.4:22.1; 2.3-fold increase	982:1009	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	11	46	theme	alpha	1070:1074	arg1	alignments					1076:1085	urease subunit alpha alignments	1055:1085	urease subunit alpha alignments	1055:1085	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	13	47	theme	dietary	1282:1288	arg1	nitrogen					1290:1297	dietary nitrogen	1282:1297	dietary nitrogen	1282:1297	The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.					
33538807	9	48	theme	same	805:808	arg1	end					810:812	the same end	801:812	the same end	801:812	To the same end, metagenome data were mined for urease-related genes.					
33538807	12	49	theme	important	1151:1159	arg1	commensal					1165:1173	an important gut commensal	1148:1173	an important gut commensal that colonizes the infant intestine	1148:1209	These experiments show how an important gut commensal that colonizes the infant intestine can metabolize urea.					
33538807	1	50	theme	health-promoting	108:123	arg1	microbiome					129:138	a health-promoting gut microbiome	106:138	a health-promoting gut microbiome in infants	106:149	Human milk stimulates a health-promoting gut microbiome in infants.					
33538807	10	51	dep	51.4:22.1	982:990	arg1	increase					1002:1009	2.3-fold increase	993:1009	51.4:22.1; 2.3-fold increase	982:1009	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	2	52	theme	microbiota	183:192	arg1	salvages					194:201	the microbiota salvages	179:201	the microbiota salvages	179:201	However, it is unclear how the microbiota salvages and processes its required nitrogen from breast milk.					
33538807	1	53	theme	gut	125:127	arg1	microbiome					129:138	a health-promoting gut microbiome	106:138	a health-promoting gut microbiome in infants	106:149	Human milk stimulates a health-promoting gut microbiome in infants.					
33538807	10	54	contain	possessed	920:928	arg1	microbiome					909:918	the breastfed infant's microbiome	886:918	the breastfed infant's microbiome	886:918	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	10	54	contain	possessed	920:928	arg2	genes					950:954	more urease-related genes	930:954	more urease-related genes	930:954	It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase).					
33538807	4	55	from	milk	391:394	arg1	abundant					373:380	abundant	373:380	abundant	373:380	Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN).					
33538807	2	56	theme	breast	244:249	arg1	milk					251:254	breast milk	244:254	breast milk	244:254	However, it is unclear how the microbiota salvages and processes its required nitrogen from breast milk.					
33538807	13	57	theme	overall	1367:1373	arg1	microbiome					1375:1384	the overall microbiome	1363:1384	the overall microbiome	1363:1384	The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.					
33538807	6	58	theme	synthetic	566:574	arg1	medium					576:581	synthetic medium	566:581	synthetic medium	566:581	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	11	59	theme	breastfed	1102:1110	arg1	infants					1112:1118	breastfed infants	1102:1118	breastfed infants	1102:1118	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	0	60	theme	positive	49:56	arg1	infantis					74:81	urease positive Bifidobacterium infantis	42:81	urease positive Bifidobacterium infantis	42:81	Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.					
33538807	6	61	from	growth	556:561	arg1	medium					576:581	synthetic medium	566:581	synthetic medium	566:581	infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium.					
33538807	5	62	theme	B.	472:473	arg1	subsp					482:486	that B. longum subsp	467:486	that B. longum subsp	467:486	We found that B. longum subsp.					
33538807	11	63	theme	106	1048:1050	arg1	total					1039:1043	a total	1037:1043	a total	1037:1043	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	0	64	theme	urease	42:47	arg1	infantis					74:81	urease positive Bifidobacterium infantis	42:81	urease positive Bifidobacterium infantis	42:81	Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.					
33538807	11	65	located	found	1088:1092	arg1	infants					1112:1118	breastfed infants	1102:1118	breastfed infants	1102:1118	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	11	65	located	found	1088:1092	arg2	total					1039:1043	a total	1037:1043	a total	1037:1043	Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants.					
33538807	9	66	theme	urease-related	846:859	arg1	genes					861:865	urease-related genes	846:865	urease-related genes	846:865	To the same end, metagenome data were mined for urease-related genes.					
32147494	5	0	theme	Stable	675:680	arg1	nanoparticles					682:694	Stable nanoparticles	675:694	Stable nanoparticles	675:694	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	8	1	from	solubilisates	1318:1330	arg1	kinetics					1290:1297	the release kinetics	1278:1297	the release kinetics from ibuprofen-CPC solubilisates	1278:1330	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles was not hindered by alginate, and was similar to the release kinetics from ibuprofen-CPC solubilisates.					
32147494	9	2	theme	micellar	1575:1582	arg1	concentration					1584:1596	its critical micellar concentration	1562:1596	its critical micellar concentration	1562:1596	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	1	3	theme	alginate	257:264	arg1	crosslinker					242:252	a crosslinker	240:252	a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles	240:341	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	5	4	theme	negative	835:842	arg1	-40 mV					860:865	-40 mV	860:865	-40 mV	860:865	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	4	theme	negative	835:842	arg1	potential					849:857	negative zeta potential	835:857	negative zeta potential (-40 mV)	835:866	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	2	5	theme	increased	378:386	arg1	solubility					388:397	increased solubility	378:397	increased solubility of a poorly soluble drug	378:422	Here, we evaluate this system for increased solubility of a poorly soluble drug.					
32147494	9	6	theme	critical	1566:1573	arg1	concentration					1584:1596	its critical micellar concentration	1562:1596	its critical micellar concentration	1562:1596	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	5	7	theme	hydrodynamic	780:791	arg1	diameter					793:800	~150 nm hydrodynamic diameter	772:800	~150 nm hydrodynamic diameter	772:800	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	4	8	theme	led	621:623	arg1	interactions					625:636	the entropy led interactions	609:636	the entropy led interactions between alginate, CPC and ibuprofen	609:672	We acquired deeper insights into the entropy led interactions between alginate, CPC and ibuprofen.					
32147494	0	9	theme	case	100:103	arg1	study					105:109	A case study	98:109	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.	0:162	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	9	10	theme	soluble	1450:1456	arg1	drugs					1458:1462	poorly soluble drugs	1443:1462	poorly soluble drugs	1443:1462	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	8	11	from	nanoparticles	1215:1227	arg1	release					1167:1173	Drug release	1162:1173	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles	1162:1227	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles was not hindered by alginate, and was similar to the release kinetics from ibuprofen-CPC solubilisates.					
32147494	0	12	theme	ibuprofen	114:122	arg1	system					156:161	ibuprofen loaded cetylpyridinium-alginate system	114:161	ibuprofen loaded cetylpyridinium-alginate system	114:161	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	6	13	theme	X-ray	944:948	arg1	scattering					950:959	small-angle X-ray scattering	932:959	small-angle X-ray scattering	932:959	Their structure was obtained using small-angle X-ray scattering, which indicated disordered micellar associates when ibuprofen was incorporated.					
32147494	1	14	theme	surfactant	195:204	arg1	CPC					232:234	CPC	232:234	CPC	232:234	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	1	14	theme	surfactant	195:204	arg1	chloride					222:229	the surfactant cetylpyridinium chloride	191:229	the surfactant cetylpyridinium chloride (CPC)	191:235	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	5	15	theme	monodispersed	803:815	arg1	distribution					817:828	monodispersed distribution	803:828	monodispersed distribution	803:828	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	6	16	theme	small-angle	932:942	arg1	scattering					950:959	small-angle X-ray scattering	932:959	small-angle X-ray scattering	932:959	Their structure was obtained using small-angle X-ray scattering, which indicated disordered micellar associates when ibuprofen was incorporated.					
32147494	3	17	theme	nanoparticle	552:563	arg1	formation					565:573	nanoparticle formation	552:573	nanoparticle formation	552:573	The aim was to use CPC for solubilisation of ibuprofen and to use the micellar associates formed for alginate complexation and nanoparticle formation.					
32147494	8	18	theme	ibuprofen-cetylpyridinium-alginate	1180:1213	arg1	nanoparticles					1215:1227	ibuprofen-cetylpyridinium-alginate nanoparticles	1180:1227	ibuprofen-cetylpyridinium-alginate nanoparticles	1180:1227	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles was not hindered by alginate, and was similar to the release kinetics from ibuprofen-CPC solubilisates.					
32147494	2	19	theme	drug	419:422	arg1	solubility					388:397	increased solubility	378:397	increased solubility of a poorly soluble drug	378:422	Here, we evaluate this system for increased solubility of a poorly soluble drug.					
32147494	4	20	theme	deeper	588:593	arg1	insights					595:602	deeper insights	588:602	deeper insights into the entropy led interactions between alginate, CPC and ibuprofen	588:672	We acquired deeper insights into the entropy led interactions between alginate, CPC and ibuprofen.					
32147494	9	21	theme	drugs	1458:1462	arg1	solubilisation					1425:1438	solubilisation	1425:1438	solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles	1425:1653	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	2	22	theme	soluble	411:417	arg1	drug					419:422	a poorly soluble drug	402:422	a poorly soluble drug	402:422	Here, we evaluate this system for increased solubility of a poorly soluble drug.					
32147494	0	23	theme	Polyelectrolyte-surfactant-complex	0:33	arg1	nanoparticles					35:47	Polyelectrolyte-surfactant-complex nanoparticles	0:47	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.	0:162	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	7	24	theme	electron	1141:1148	arg1	microscopy					1150:1159	transmission electron microscopy	1128:1159	transmission electron microscopy	1128:1159	This resulted in nanoparticles with a complex nanostructured composition, as shown by transmission electron microscopy.					
32147494	1	25	theme	cetylpyridinium	206:220	arg1	CPC					232:234	CPC	232:234	CPC	232:234	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	1	25	theme	cetylpyridinium	206:220	arg1	chloride					222:229	the surfactant cetylpyridinium chloride	191:229	the surfactant cetylpyridinium chloride (CPC)	191:235	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	8	26	theme	ibuprofen-CPC	1304:1316	arg1	solubilisates					1318:1330	ibuprofen-CPC solubilisates	1304:1330	ibuprofen-CPC solubilisates	1304:1330	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles was not hindered by alginate, and was similar to the release kinetics from ibuprofen-CPC solubilisates.					
32147494	1	27	theme	stable	287:292	arg1	nanoparticles					329:341	stable polyelectrolyte-surfactant-complex nanoparticles	287:341	stable polyelectrolyte-surfactant-complex nanoparticles	287:341	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	0	28	theme	cetylpyridinium-alginate	131:154	arg1	system					156:161	ibuprofen loaded cetylpyridinium-alginate system	114:161	ibuprofen loaded cetylpyridinium-alginate system	114:161	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	9	29	theme	innovative	1339:1348	arg1	carriers					1350:1357	These innovative carriers	1333:1357	These innovative carriers	1333:1357	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	5	30	theme	%	876:876	arg1	loading					888:894	34% ibuprofen loading	874:894	34% ibuprofen loading	874:894	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	31	theme	34	874:875	arg1	%					876:876	%	876:876	%	876:876	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	1	32	theme	polyelectrolyte-surfactant-complex	294:327	arg1	nanoparticles					329:341	stable polyelectrolyte-surfactant-complex nanoparticles	287:341	stable polyelectrolyte-surfactant-complex nanoparticles	287:341	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	0	33	theme	loaded	124:129	arg1	system					156:161	ibuprofen loaded cetylpyridinium-alginate system	114:161	ibuprofen loaded cetylpyridinium-alginate system	114:161	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	5	34	theme	ibuprofen	878:886	arg1	loading					888:894	34% ibuprofen loading	874:894	34% ibuprofen loading	874:894	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	35	theme	zeta	844:847	arg1	-40 mV					860:865	-40 mV	860:865	-40 mV	860:865	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	35	theme	zeta	844:847	arg1	potential					849:857	negative zeta potential	835:857	negative zeta potential (-40 mV)	835:866	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	1	36	theme	nanoparticles	329:341	arg1	formation					274:282	the formation	270:282	the formation of stable polyelectrolyte-surfactant-complex nanoparticles	270:341	Previously, we reported on the surfactant cetylpyridinium chloride (CPC) as a crosslinker of alginate for the formation of stable polyelectrolyte-surfactant-complex nanoparticles.					
32147494	7	37	theme	transmission	1128:1139	arg1	microscopy					1150:1159	transmission electron microscopy	1128:1159	transmission electron microscopy	1128:1159	This resulted in nanoparticles with a complex nanostructured composition, as shown by transmission electron microscopy.					
32147494	8	38	theme	Drug	1162:1165	arg1	release					1167:1173	Drug release	1162:1173	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles	1162:1227	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles was not hindered by alginate, and was similar to the release kinetics from ibuprofen-CPC solubilisates.					
32147494	5	39	with	ratios	759:764	arg1	-40 mV					860:865	-40 mV	860:865	-40 mV	860:865	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	39	with	ratios	759:764	arg1	potential					849:857	negative zeta potential	835:857	negative zeta potential (-40 mV)	835:866	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	39	with	ratios	759:764	arg1	distribution					817:828	monodispersed distribution	803:828	monodispersed distribution	803:828	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	39	with	ratios	759:764	arg1	diameter					793:800	~150 nm hydrodynamic diameter	772:800	~150 nm hydrodynamic diameter	772:800	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	0	40	theme	delivery	54:61	arg1	platform					63:70	a delivery platform	52:70	a delivery platform for poorly soluble drugs	52:95	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	0	41	theme	system	156:161	arg1	study					105:109	A case study	98:109	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.	0:162	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	7	42	theme	nanostructured	1088:1101	arg1	composition					1103:1113	a complex nanostructured composition	1078:1113	a complex nanostructured composition	1078:1113	This resulted in nanoparticles with a complex nanostructured composition, as shown by transmission electron microscopy.					
32147494	3	43	theme	ibuprofen	470:478	arg1	solubilisation					452:465	solubilisation	452:465	solubilisation of ibuprofen	452:478	The aim was to use CPC for solubilisation of ibuprofen and to use the micellar associates formed for alginate complexation and nanoparticle formation.					
32147494	9	44	theme	polyelectrolyte-surfactant	1372:1397	arg1	complexes					1399:1407	polyelectrolyte-surfactant complexes	1372:1407	polyelectrolyte-surfactant complexes	1372:1407	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	3	45	theme	micellar	495:502	arg1	associates					504:513	the micellar associates	491:513	the micellar associates formed for alginate complexation and nanoparticle formation	491:573	The aim was to use CPC for solubilisation of ibuprofen and to use the micellar associates formed for alginate complexation and nanoparticle formation.					
32147494	5	46	theme	~150 nm	772:778	arg1	diameter					793:800	~150 nm hydrodynamic diameter	772:800	~150 nm hydrodynamic diameter	772:800	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	5	47	theme	limited	715:721	arg1	ratios					759:764	limited surfactant-to-polyelectrolyte molar ratios	715:764	limited surfactant-to-polyelectrolyte molar ratios	715:764	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	6	48	theme	micellar	989:996	arg1	associates					998:1007	disordered micellar associates	978:1007	disordered micellar associates	978:1007	Their structure was obtained using small-angle X-ray scattering, which indicated disordered micellar associates when ibuprofen was incorporated.					
32147494	5	49	theme	surfactant-to-polyelectrolyte	723:751	arg1	ratios					759:764	limited surfactant-to-polyelectrolyte molar ratios	715:764	limited surfactant-to-polyelectrolyte molar ratios	715:764	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	7	50	theme	complex	1080:1086	arg1	composition					1103:1113	a complex nanostructured composition	1078:1113	a complex nanostructured composition	1078:1113	This resulted in nanoparticles with a complex nanostructured composition, as shown by transmission electron microscopy.					
32147494	9	51	theme	drug	1533:1536	arg1	solubility					1515:1524	the solubility	1511:1524	the solubility of the drug	1511:1536	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	5	52	theme	molar	753:757	arg1	ratios					759:764	limited surfactant-to-polyelectrolyte molar ratios	715:764	limited surfactant-to-polyelectrolyte molar ratios	715:764	Stable nanoparticles were formed across limited surfactant-to-polyelectrolyte molar ratios, with ~150 nm hydrodynamic diameter, monodispersed distribution, and negative zeta potential (-40 mV), with 34% ibuprofen loading.					
32147494	8	53	theme	release	1282:1288	arg1	kinetics					1290:1297	the release kinetics	1278:1297	the release kinetics from ibuprofen-CPC solubilisates	1278:1330	Drug release from ibuprofen-cetylpyridinium-alginate nanoparticles was not hindered by alginate, and was similar to the release kinetics from ibuprofen-CPC solubilisates.					
32147494	9	54	used	used	1416:1419	arg2	complexes					1399:1407	polyelectrolyte-surfactant complexes	1372:1407	polyelectrolyte-surfactant complexes	1372:1407	These innovative carriers developed as polyelectrolyte-surfactant complexes can be used for solubilisation of poorly soluble drugs, where the surfactant simultaneously increases the solubility of the drug at concentrations below its critical micellar concentration and crosslinks the polyelectrolyte to form nanoparticles.					
32147494	0	55	dep	nanoparticles	35:47	arg1	study					105:109	A case study	98:109	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.	0:162	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	3	56	theme	alginate	526:533	arg1	complexation					535:546	alginate complexation	526:546	alginate complexation	526:546	The aim was to use CPC for solubilisation of ibuprofen and to use the micellar associates formed for alginate complexation and nanoparticle formation.					
32147494	7	57	with	nanoparticles	1059:1071	arg1	composition					1103:1113	a complex nanostructured composition	1078:1113	a complex nanostructured composition	1078:1113	This resulted in nanoparticles with a complex nanostructured composition, as shown by transmission electron microscopy.					
32147494	0	58	theme	soluble	83:89	arg1	drugs					91:95	poorly soluble drugs	76:95	poorly soluble drugs	76:95	Polyelectrolyte-surfactant-complex nanoparticles as a delivery platform for poorly soluble drugs: A case study of ibuprofen loaded cetylpyridinium-alginate system.					
32147494	6	59	theme	disordered	978:987	arg1	associates					998:1007	disordered micellar associates	978:1007	disordered micellar associates	978:1007	Their structure was obtained using small-angle X-ray scattering, which indicated disordered micellar associates when ibuprofen was incorporated.					
33359810	7	0	theme	hydrophilic	1208:1218	arg1	nature					1220:1225	the hydrophilic nature	1204:1225	the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility	1204:1296	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
33359810	4	1	theme	energy	723:728	arg1	dissipation					730:740	the more effective energy dissipation	704:740	the more effective energy dissipation of hydrogen bonds	704:758	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	5	2	theme	fracture	863:870	arg1	strength					872:879	compressive fracture strength	851:879	compressive fracture strength	851:879	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	7	3	theme	interface	1274:1282	arg1	compatibility					1284:1296	good interface compatibility	1269:1296	good interface compatibility	1269:1296	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
33359810	8	4	theme	related	1523:1529	arg1	applications					1531:1542	many biomaterial related applications	1506:1542	many biomaterial related applications	1506:1542	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	0	5	theme	cellulose	69:77	arg1	nanofibers					79:88	bacterial cellulose nanofibers	59:88	bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility	59:152	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	7	6	theme	γ-PGA	1177:1181	arg1	hydrogels					1183:1191	the γ-PGA hydrogels	1173:1191	the γ-PGA hydrogels	1173:1191	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
33359810	8	7	theme	excellent	1384:1392	arg1	cytocompatibility					1394:1410	excellent cytocompatibility	1384:1410	excellent cytocompatibility	1384:1410	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	6	8	theme	hydrogels	1044:1052	arg1	strength					1013:1020	the tensile strength	1001:1020	the tensile strength of γ-PGA/BC composite hydrogels	1001:1052	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	0	9	theme	superior	101:108	arg1	properties					121:130	superior mechanical properties	101:130	superior mechanical properties	101:130	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	8	10	theme	promising	1482:1490	arg1	materials					1492:1500	promising materials	1482:1500	promising materials for many biomaterial related applications	1482:1542	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	8	10	theme	promising	1482:1490	arg1	hydrogels					1457:1465	the γ-PGA/BC composite hydrogels	1434:1465	the γ-PGA/BC composite hydrogels	1434:1465	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	6	11	theme	composite	1034:1042	arg1	hydrogels					1044:1052	γ-PGA/BC composite hydrogels	1025:1052	γ-PGA/BC composite hydrogels	1025:1052	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	7	12	theme	BC	1240:1241	arg1	nanofillers					1243:1253	BC nanofillers	1240:1253	BC nanofillers	1240:1253	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
33359810	2	13	with	hydrogels	401:409	arg1	properties					437:446	excellent mechanical properties	416:446	excellent mechanical properties	416:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	4	14	theme	hydrogel	798:805	arg1	matrix					807:812	γ-PGA hydrogel matrix	792:812	γ-PGA hydrogel matrix	792:812	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	6	15	theme	γ-PGA/BC	1025:1032	arg1	hydrogels					1044:1052	γ-PGA/BC composite hydrogels	1025:1052	γ-PGA/BC composite hydrogels	1025:1052	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	4	16	theme	bonds	754:758	arg1	dissipation					730:740	the more effective energy dissipation	704:740	the more effective energy dissipation of hydrogen bonds	704:758	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	4	17	theme	excellent	664:672	arg1	strength					674:681	excellent strength	664:681	excellent strength	664:681	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	5	18	theme	γ-PGA/BC	902:909	arg1	hydrogels					921:929	the γ-PGA/BC composite hydrogels	898:929	the γ-PGA/BC composite hydrogels	898:929	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	6	19	theme	single	1099:1104	arg1	hydrogels					1114:1122	γ-PGA single network hydrogels	1093:1122	γ-PGA single network hydrogels	1093:1122	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	0	20	theme	mechanical	110:119	arg1	properties					121:130	superior mechanical properties	101:130	superior mechanical properties	101:130	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	3	21	theme	covalent	555:562	arg1	cross-linking					564:576	the covalent cross-linking	551:576	the covalent cross-linking of γ-PGA	551:585	The γ-PGA/BC composite hydrogels were obtained by the covalent cross-linking of γ-PGA in the BC nanofibers suspensions.					
33359810	2	22	theme	BC	387:388	arg1	hydrogels					401:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels	331:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties	331:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	2	23	theme	poly	335:338	arg1	hydrogels					401:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels	331:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties	331:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	1	24	theme	promising	200:208	arg1	biomaterial					210:220	promising biomaterial	200:220	promising biomaterial	200:220	Natural polymer hydrogels are expected to be promising biomaterial because of its excellent biocompatibility and biodegradability, but they are soft and easily broken.					
33359810	0	25	theme	Bio-based	0:8	arg1	poly					10:13	Bio-based poly	0:13	Bio-based poly (γ-glutamic acid)	0:31	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	0	25	theme	Bio-based	0:8	arg1	acid					27:30	γ-glutamic acid	16:30	γ-glutamic acid	16:30	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	4	26	theme	BC	774:775	arg1	nanofibers					777:786	BC nanofibers	774:786	BC nanofibers	774:786	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	2	27	theme	cellulose	376:384	arg1	hydrogels					401:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels	331:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties	331:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	0	28	theme	γ-glutamic	16:25	arg1	poly					10:13	Bio-based poly	0:13	Bio-based poly (γ-glutamic acid)	0:31	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	0	28	theme	γ-glutamic	16:25	arg1	acid					27:30	γ-glutamic acid	16:30	γ-glutamic acid	16:30	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	8	29	theme	composite	1356:1364	arg1	hydrogels					1366:1374	γ-PGA/BC composite hydrogels	1347:1374	γ-PGA/BC composite hydrogels	1347:1374	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	8	30	theme	γ-PGA/BC	1347:1354	arg1	hydrogels					1366:1374	γ-PGA/BC composite hydrogels	1347:1374	γ-PGA/BC composite hydrogels	1347:1374	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	3	31	theme	γ-PGA	581:585	arg1	cross-linking					564:576	the covalent cross-linking	551:576	the covalent cross-linking of γ-PGA	551:585	The γ-PGA/BC composite hydrogels were obtained by the covalent cross-linking of γ-PGA in the BC nanofibers suspensions.					
33359810	2	32	theme	excellent	416:424	arg1	properties					437:446	excellent mechanical properties	416:446	excellent mechanical properties	416:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	2	33	theme	mechanical	426:435	arg1	properties					437:446	excellent mechanical properties	416:446	excellent mechanical properties	416:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	6	34	theme	tensile	1005:1011	arg1	strength					1013:1020	the tensile strength	1001:1020	the tensile strength of γ-PGA/BC composite hydrogels	1001:1052	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	8	35	theme	biomaterial	1511:1521	arg1	applications					1531:1542	many biomaterial related applications	1506:1542	many biomaterial related applications	1506:1542	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	8	36	theme	composite	1447:1455	arg1	materials					1492:1500	promising materials	1482:1500	promising materials for many biomaterial related applications	1482:1542	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	8	36	theme	composite	1447:1455	arg1	hydrogels					1457:1465	the γ-PGA/BC composite hydrogels	1434:1465	the γ-PGA/BC composite hydrogels	1434:1465	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	1	37	theme	polymer	163:169	arg1	hydrogels					171:179	Natural polymer hydrogels	155:179	Natural polymer hydrogels	155:179	Natural polymer hydrogels are expected to be promising biomaterial because of its excellent biocompatibility and biodegradability, but they are soft and easily broken.					
33359810	7	38	theme	nanofillers	1243:1253	arg1	nature					1220:1225	the hydrophilic nature	1204:1225	the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility	1204:1296	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
33359810	4	39	theme	hydrogen	745:752	arg1	bonds					754:758	hydrogen bonds	745:758	hydrogen bonds	745:758	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	6	40	theme	γ-PGA	1093:1097	arg1	hydrogels					1114:1122	γ-PGA single network hydrogels	1093:1122	γ-PGA single network hydrogels	1093:1122	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	5	41	theme	composite	911:919	arg1	hydrogels					921:929	the γ-PGA/BC composite hydrogels	898:929	the γ-PGA/BC composite hydrogels	898:929	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	4	42	theme	toughness	687:695	arg1	network					760:766	excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network	664:766	excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network	664:766	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	2	43	theme	bacterial	480:488	arg1	cellulose					490:498	bacterial cellulose	480:498	bacterial cellulose	480:498	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	8	44	theme	tests	1326:1330	arg1	result					1303:1308	The result	1299:1308	The result of cytotoxicity tests	1299:1330	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	0	45	dep	hydrogels	33:41	arg1	reinforced					43:52	reinforced	43:52	hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility	33:152	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	1	46	theme	excellent	237:245	arg1	biocompatibility					247:262	its excellent biocompatibility	233:262	its excellent biocompatibility	233:262	Natural polymer hydrogels are expected to be promising biomaterial because of its excellent biocompatibility and biodegradability, but they are soft and easily broken.					
33359810	5	47	theme	hydrogels	921:929	arg1	toughness					885:893	toughness	885:893	toughness	885:893	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	5	47	theme	hydrogels	921:929	arg1	strength					872:879	compressive fracture strength	851:879	compressive fracture strength	851:879	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	8	48	theme	γ-PGA/BC	1438:1445	arg1	materials					1492:1500	promising materials	1482:1500	promising materials for many biomaterial related applications	1482:1542	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	8	48	theme	γ-PGA/BC	1438:1445	arg1	hydrogels					1457:1465	the γ-PGA/BC composite hydrogels	1434:1465	the γ-PGA/BC composite hydrogels	1434:1465	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	3	49	theme	nanofibers	597:606	arg1	suspensions					608:618	the BC nanofibers suspensions	590:618	the BC nanofibers suspensions	590:618	The γ-PGA/BC composite hydrogels were obtained by the covalent cross-linking of γ-PGA in the BC nanofibers suspensions.					
33359810	3	50	theme	γ-PGA/BC	505:512	arg1	hydrogels					524:532	The γ-PGA/BC composite hydrogels	501:532	The γ-PGA/BC composite hydrogels	501:532	The γ-PGA/BC composite hydrogels were obtained by the covalent cross-linking of γ-PGA in the BC nanofibers suspensions.					
33359810	8	51	theme	many	1506:1509	arg1	applications					1531:1542	many biomaterial related applications	1506:1542	many biomaterial related applications	1506:1542	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	3	52	theme	composite	514:522	arg1	hydrogels					524:532	The γ-PGA/BC composite hydrogels	501:532	The γ-PGA/BC composite hydrogels	501:532	The γ-PGA/BC composite hydrogels were obtained by the covalent cross-linking of γ-PGA in the BC nanofibers suspensions.					
33359810	5	53	dep	strength	872:879	arg1	The					847:849	The	847:849	The	847:849	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	2	54	theme	γ-glutamic	341:350	arg1	acid					352:355	γ-glutamic acid	341:355	γ-glutamic acid	341:355	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	2	54	theme	γ-glutamic	341:350	arg1	poly					335:338	poly	335:338	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties	331:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	4	55	theme	γ-PGA	792:796	arg1	matrix					807:812	γ-PGA hydrogel matrix	792:812	γ-PGA hydrogel matrix	792:812	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	4	56	theme	composite	634:642	arg1	hydrogels					644:652	The γ-PGA/BC composite hydrogels	621:652	The γ-PGA/BC composite hydrogels	621:652	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	6	57	theme	network	1106:1112	arg1	hydrogels					1114:1122	γ-PGA single network hydrogels	1093:1122	γ-PGA single network hydrogels	1093:1122	Additionally, the tensile strength of γ-PGA/BC composite hydrogels were improved 8.16 times compared with γ-PGA single network hydrogels.					
33359810	1	58	theme	Natural	155:161	arg1	hydrogels					171:179	Natural polymer hydrogels	155:179	Natural polymer hydrogels	155:179	Natural polymer hydrogels are expected to be promising biomaterial because of its excellent biocompatibility and biodegradability, but they are soft and easily broken.					
33359810	4	59	theme	γ-PGA/BC	625:632	arg1	hydrogels					644:652	The γ-PGA/BC composite hydrogels	621:652	The γ-PGA/BC composite hydrogels	621:652	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	0	60	theme	bacterial	59:67	arg1	nanofibers					79:88	bacterial cellulose nanofibers	59:88	bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility	59:152	Bio-based poly (γ-glutamic acid) hydrogels reinforced with bacterial cellulose nanofibers exhibiting superior mechanical properties and cytocompatibility.					
33359810	2	61	theme	composite	391:399	arg1	hydrogels					401:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels	331:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties	331:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	8	62	theme	cytotoxicity	1313:1324	arg1	tests					1326:1330	cytotoxicity tests	1313:1330	cytotoxicity tests	1313:1330	The result of cytotoxicity tests indicated that γ-PGA/BC composite hydrogels present excellent cytocompatibility, which suggested that the γ-PGA/BC composite hydrogels could serve as promising materials for many biomaterial related applications.					
33359810	4	63	theme	strength	674:681	arg1	network					760:766	excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network	664:766	excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network	664:766	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	2	64	theme	/bacterial	365:374	arg1	hydrogels					401:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels	331:409	the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties	331:446	Herein, the poly (γ-glutamic acid) (γ-PGA)/bacterial cellulose (BC) composite hydrogels with excellent mechanical properties were constructed by introducing bacterial cellulose.					
33359810	4	65	theme	due	697:699	arg1	network					760:766	excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network	664:766	excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network	664:766	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	7	66	theme	good	1269:1272	arg1	compatibility					1284:1296	good interface compatibility	1269:1296	good interface compatibility	1269:1296	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
33359810	5	67	theme	compressive	851:861	arg1	strength					872:879	compressive fracture strength	851:879	compressive fracture strength	851:879	The compressive fracture strength and toughness of the γ-PGA/BC composite hydrogels could reach up to 5.72 MPa and 0.42 MJ/m3 respectively.					
33359810	4	68	theme	effective	713:721	arg1	dissipation					730:740	the more effective energy dissipation	704:740	the more effective energy dissipation of hydrogen bonds	704:758	The γ-PGA/BC composite hydrogels exhibited excellent strength and toughness due to the more effective energy dissipation of hydrogen bonds network among BC nanofibers and γ-PGA hydrogel matrix and BC also acts as an enhancer.					
33359810	7	69	theme	γ-PGA	1230:1234	arg1	nature					1220:1225	the hydrophilic nature	1204:1225	the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility	1204:1296	More significantly, BC could disperse evenly in the γ-PGA hydrogels because of the hydrophilic nature of γ-PGA and BC nanofillers, which led to good interface compatibility.					
32475667	6	0	theme	paired	939:944	arg1	C					959:959	C	959:959	C	959:959	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	6	0	theme	paired	939:944	arg1	cow					954:956	each paired control cow	934:956	each paired control cow (C)	934:960	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	12	1	theme	systemic	1877:1884	arg1	effects					1886:1892	systemic effects	1877:1892	systemic effects	1877:1892	Concomitant with changes in milk, systemic effects were also observed in blood.					
32475667	10	2	theme	local	1543:1547	arg1	response					1549:1556	a local response	1541:1556	a local response to LPS	1541:1563	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	9	3	theme	systemic	1305:1312	arg1	effects					1314:1320	systemic effects	1305:1320	systemic effects	1305:1320	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	12	4	located	observed	1904:1911	arg2	effects					1886:1892	systemic effects	1877:1892	systemic effects	1877:1892	Concomitant with changes in milk, systemic effects were also observed in blood.					
32475667	12	4	located	observed	1904:1911	arg1	blood					1916:1920	blood	1916:1920	blood	1916:1920	Concomitant with changes in milk, systemic effects were also observed in blood.					
32475667	2	5	theme	adjacent	280:287	arg1	glands					301:306	adjacent, uninfected glands	280:306	adjacent, uninfected glands	280:306	However, mastitis in one quarter may affect function of adjacent, uninfected glands.					
32475667	15	6	theme	milk	2370:2373	arg1	production					2375:2384	milk production	2370:2384	milk production	2370:2384	Depending on the component, effects were local, systemic, or both, suggesting involvement of multiple different mediators that collectively result in systemic inhibition of milk production.					
32475667	5	7	theme	selected	776:783	arg1	half					791:794	a randomly selected udder half	765:794	a randomly selected udder half	765:794	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	0	8	theme	milk	79:82	arg1	components					84:93	milk components	79:93	milk components in lactating bovine mammary glands	79:128	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	5	9	theme	half	791:794	arg1	front					735:739	the front	731:739	the front	731:739	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	5	9	theme	half	791:794	arg1	quarter					754:760	the rear quarter	745:760	the rear quarter of a randomly selected udder half	745:794	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	9	10	theme	h	1341:1341	arg1	post-infusion					1343:1355	3 h post-infusion	1339:1355	3 h post-infusion	1339:1355	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	4	11	theme	somatic	618:624	arg1	SCC					638:640	SCC	638:640	SCC	638:640	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	4	11	theme	somatic	618:624	arg1	count					631:635	cow-level somatic cell count	608:635	cow-level somatic cell count (SCC)	608:641	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	1	12	theme	distinct	208:215	arg1	quarter					136:142	Each quarter	131:142	Each quarter of the bovine mammary gland	131:170	Each quarter of the bovine mammary gland is an anatomically and functionally distinct gland.					
32475667	1	12	theme	distinct	208:215	arg1	gland					217:221	an anatomically and functionally distinct gland	175:221	an anatomically and functionally distinct gland	175:221	Each quarter of the bovine mammary gland is an anatomically and functionally distinct gland.					
32475667	14	13	theme	unilateral	2108:2117	arg1	infusion					2123:2130	unilateral LPS infusion	2108:2130	unilateral LPS infusion	2108:2130	In summary, unilateral LPS infusion induced distinct, time-dependent effects on each milk component.					
32475667	0	14	from	components	84:93	arg1	glands					123:128	lactating bovine mammary glands	98:128	lactating bovine mammary glands	98:128	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	6	15	theme	udder	1026:1030	arg1	halves					1032:1037	udder halves	1026:1037	udder halves	1026:1037	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	15	16	theme	different	2299:2307	arg1	mediators					2309:2317	multiple different mediators	2290:2317	multiple different mediators that collectively result in systemic inhibition of milk production	2290:2384	Depending on the component, effects were local, systemic, or both, suggesting involvement of multiple different mediators that collectively result in systemic inhibition of milk production.					
32475667	5	17	theme	µg	823:824	arg1	infusion					808:815	an infusion	805:815	an infusion of 50 µg of LPS in 10 mL of saline (T-L)	805:856	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	12	18	dep	observed	1904:1911	arg1	Concomitant					1843:1853	Concomitant	1843:1853	Concomitant	1843:1853	Concomitant with changes in milk, systemic effects were also observed in blood.					
32475667	5	19	theme	LPS	829:831	arg1	µg					823:824	50 µg	820:824	50 µg of LPS	820:831	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	9	20	theme	foremilk	1396:1403	arg1	concentration					1409:1421	foremilk fat concentration	1396:1421	foremilk fat concentration	1396:1421	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	7	21	theme	front	1109:1113	arg1	quarters					1115:1122	front quarters	1109:1122	front quarters	1109:1122	Cows were quarter milked twice daily, with foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions.					
32475667	4	22	theme	milk	648:651	arg1	yield					653:657	milk yield	648:657	milk yield	648:657	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	1	23	theme	bovine	151:156	arg1	gland					166:170	the bovine mammary gland	147:170	the bovine mammary gland	147:170	Each quarter of the bovine mammary gland is an anatomically and functionally distinct gland.					
32475667	5	24	from	infusion	808:815	arg1	mL					839:840	10 mL	836:840	10 mL of saline (T-L)	836:856	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	1	25	theme	gland	166:170	arg1	quarter					136:142	Each quarter	131:142	Each quarter of the bovine mammary gland	131:170	Each quarter of the bovine mammary gland is an anatomically and functionally distinct gland.					
32475667	1	25	theme	gland	166:170	arg1	gland					217:221	an anatomically and functionally distinct gland	175:221	an anatomically and functionally distinct gland	175:221	Each quarter of the bovine mammary gland is an anatomically and functionally distinct gland.					
32475667	8	26	theme	average	1193:1199	arg1	yield					1206:1210	average milk yield	1193:1210	average milk yield in T-L and T-S quarters	1193:1234	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	0	27	theme	lactating	98:106	arg1	glands					123:128	lactating bovine mammary glands	98:128	lactating bovine mammary glands	98:128	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	7	28	dep	milked	1058:1063	arg1	quarter					1050:1056	quarter	1050:1056	quarter	1050:1056	Cows were quarter milked twice daily, with foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions.					
32475667	3	29	theme	potential	343:351	arg1	mediators					353:361	potential mediators	343:361	potential mediators	343:361	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	0	30	theme	mammary	115:121	arg1	glands					123:128	lactating bovine mammary glands	98:128	lactating bovine mammary glands	98:128	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	8	31	theme	T-L	1215:1217	arg1	quarters					1227:1234	T-L and T-S quarters	1215:1234	T-L and T-S quarters	1215:1234	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	11	32	theme	systemic	1769:1776	arg1	effect					1778:1783	then a systemic effect	1762:1783	then a systemic effect	1762:1783	Lactose concentration showed a local effect at 6 h, being lower in T-L than in T-S, and then a systemic effect at 12 h, being lower in both T-L and T-S than C quarters.					
32475667	3	33	theme	mammary	418:424	arg1	gland					426:430	the mammary gland	414:430	the mammary gland	414:430	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	0	34	theme	Intramammary	0:11	arg1	infusion					32:39	Intramammary lipopolysaccharide infusion	0:39	Intramammary lipopolysaccharide infusion	0:39	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	13	35	theme	Plasma	1923:1928	arg1	potential					1942:1950	Plasma antioxidant potential	1923:1950	Plasma antioxidant potential	1923:1950	Plasma antioxidant potential and glucose concentration were lower in T cows than in C cows at 6 or 12 h, respectively, although neither variable remained different at 24 h.					
32475667	10	36	from	SCC	1465:1467	arg1	foremilk					1522:1529	foremilk	1522:1529	foremilk	1522:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	3	37	theme	effects	372:378	arg1	mechanisms					328:337	mechanisms	328:337	mechanisms	328:337	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	3	37	theme	effects	372:378	arg1	mediators					353:361	potential mediators	343:361	potential mediators	343:361	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	8	38	theme	T-S	1223:1225	arg1	quarters					1227:1234	T-L and T-S quarters	1215:1234	T-L and T-S quarters	1215:1234	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	10	39	from	concentrations	1473:1486	arg1	foremilk					1522:1529	foremilk	1522:1529	foremilk	1522:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	3	40	dep	mechanisms	328:337	arg1	the					324:326	the	324:326	the	324:326	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	3	41	theme	lipopolysaccharide	448:465	arg1	challenge					473:481	intramammary lipopolysaccharide (LPS) challenge	435:481	intramammary lipopolysaccharide (LPS) challenge	435:481	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	0	42	theme	local	49:53	arg1	effects					68:74	local and systemic effects	49:74	local and systemic effects	49:74	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	3	43	theme	early	395:399	arg1	responses					401:409	early responses	395:409	early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge	395:481	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	8	44	from	yield	1206:1210	arg1	quarters					1227:1234	T-L and T-S quarters	1215:1234	T-L and T-S quarters	1215:1234	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	14	45	theme	milk	2181:2184	arg1	component					2186:2194	each milk component	2176:2194	each milk component	2176:2194	In summary, unilateral LPS infusion induced distinct, time-dependent effects on each milk component.					
32475667	4	46	theme	multiparous	539:549	arg1	cows					551:554	Ten multiparous cows	535:554	Ten multiparous cows over 70 d in milk	535:572	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	10	47	from	h	1646:1646	arg1	T-L					1651:1653	T-L	1651:1653	T-L	1651:1653	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	11	48	theme	C	1831:1831	arg1	quarters					1833:1840	C quarters	1831:1840	C quarters	1831:1840	Lactose concentration showed a local effect at 6 h, being lower in T-L than in T-S, and then a systemic effect at 12 h, being lower in both T-L and T-S than C quarters.					
32475667	10	49	theme	protein	1511:1517	arg1	SCC					1465:1467	SCC	1465:1467	SCC	1465:1467	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	10	49	theme	protein	1511:1517	arg1	concentrations					1473:1486	concentrations	1473:1486	concentrations of l-lactate and total protein in foremilk	1473:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	3	50	theme	systemic	517:524	arg1	effects					526:532	local and systemic effects	507:532	local and systemic effects	507:532	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	9	51	theme	T	1297:1297	arg1	cows					1299:1302	T cows	1297:1302	T cows	1297:1302	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	6	52	theme	control	946:952	arg1	C					959:959	C	959:959	C	959:959	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	6	52	theme	control	946:952	arg1	cow					954:956	each paired control cow	934:956	each paired control cow (C)	934:960	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	8	53	theme	levels	1271:1276	arg1	levels					1271:1276	pre-infusion levels	1258:1276	pre-infusion levels	1258:1276	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	8	53	theme	levels	1271:1276	arg1	%					1253:1253	23 and 32%	1244:1253	23 and 32% of pre-infusion levels	1244:1276	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	3	54	theme	local	507:511	arg1	effects					526:532	local and systemic effects	507:532	local and systemic effects	507:532	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	14	55	theme	distinct	2140:2147	arg1	effects					2165:2171	distinct, time-dependent effects	2140:2171	distinct, time-dependent effects	2140:2171	In summary, unilateral LPS infusion induced distinct, time-dependent effects on each milk component.					
32475667	12	56	with	Concomitant	1843:1853	arg1	changes					1860:1866	changes	1860:1866	changes in milk	1860:1874	Concomitant with changes in milk, systemic effects were also observed in blood.					
32475667	10	57	from	protein	1511:1517	arg1	foremilk					1522:1529	foremilk	1522:1529	foremilk	1522:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	7	58	theme	relative	1161:1168	arg1	h					1159:1159	-24, 0, 3, 6, 12, and 24 h	1134:1159	-24, 0, 3, 6, 12, and 24 h relative to infusions	1134:1181	Cows were quarter milked twice daily, with foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions.					
32475667	11	59	theme	local	1705:1709	arg1	effect					1711:1716	a local effect	1703:1716	a local effect	1703:1716	Lactose concentration showed a local effect at 6 h, being lower in T-L than in T-S, and then a systemic effect at 12 h, being lower in both T-L and T-S than C quarters.					
32475667	10	60	from	foremilk	1522:1529	arg1	SCC					1465:1467	SCC	1465:1467	SCC	1465:1467	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	10	60	from	foremilk	1522:1529	arg1	concentrations					1473:1486	concentrations	1473:1486	concentrations of l-lactate and total protein in foremilk	1473:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	6	61	theme	saline	987:992	arg1	mL					981:982	10 mL	978:982	10 mL of saline (C-S)	978:998	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	6	61	theme	saline	987:992	arg1	infusion					1006:1013	no infusion	1003:1013	no infusion (C-N) into udder halves	1003:1037	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	6	61	theme	saline	987:992	arg1	C-N					1016:1018	C-N	1016:1018	C-N	1016:1018	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	5	62	theme	udder	785:789	arg1	half					791:794	a randomly selected udder half	765:794	a randomly selected udder half	765:794	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	9	63	theme	3	1339:1339	arg1	h					1341:1341	h	1341:1341	h	1341:1341	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	0	64	theme	systemic	59:66	arg1	effects					68:74	local and systemic effects	49:74	local and systemic effects	49:74	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	2	65	theme	glands	301:306	arg1	function					268:275	function	268:275	function of adjacent, uninfected glands	268:306	However, mastitis in one quarter may affect function of adjacent, uninfected glands.					
32475667	5	66	theme	rear	749:752	arg1	quarter					754:760	the rear quarter	745:760	the rear quarter of a randomly selected udder half	745:794	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	14	67	theme	LPS	2119:2121	arg1	infusion					2123:2130	unilateral LPS infusion	2108:2130	unilateral LPS infusion	2108:2130	In summary, unilateral LPS infusion induced distinct, time-dependent effects on each milk component.					
32475667	15	68	theme	systemic	2347:2354	arg1	inhibition					2356:2365	systemic inhibition	2347:2365	systemic inhibition of milk production	2347:2384	Depending on the component, effects were local, systemic, or both, suggesting involvement of multiple different mediators that collectively result in systemic inhibition of milk production.					
32475667	6	69	dep	saline	987:992	arg1	C-S					995:997	C-S	995:997	C-S	995:997	Similarly, each paired control cow (C) received either 10 mL of saline (C-S) or no infusion (C-N) into udder halves.					
32475667	13	70	theme	C	2007:2007	arg1	cows					2009:2012	C cows	2007:2012	C cows	2007:2012	Plasma antioxidant potential and glucose concentration were lower in T cows than in C cows at 6 or 12 h, respectively, although neither variable remained different at 24 h.					
32475667	9	71	theme	rectal	1360:1365	arg1	temperature					1367:1377	rectal temperature	1360:1377	rectal temperature	1360:1377	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	4	72	theme	cell	626:629	arg1	SCC					638:640	SCC	638:640	SCC	638:640	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	4	72	theme	cell	626:629	arg1	count					631:635	cow-level somatic cell count	608:635	cow-level somatic cell count (SCC)	608:641	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	15	73	theme	multiple	2290:2297	arg1	mediators					2309:2317	multiple different mediators	2290:2317	multiple different mediators that collectively result in systemic inhibition of milk production	2290:2384	Depending on the component, effects were local, systemic, or both, suggesting involvement of multiple different mediators that collectively result in systemic inhibition of milk production.					
32475667	13	74	from	h	2025:2025	arg1	cows					2009:2012	C cows	2007:2012	C cows	2007:2012	Plasma antioxidant potential and glucose concentration were lower in T cows than in C cows at 6 or 12 h, respectively, although neither variable remained different at 24 h.					
32475667	14	75	dep	distinct	2140:2147	arg1	time-dependent					2150:2163	time-dependent	2150:2163	time-dependent	2150:2163	In summary, unilateral LPS infusion induced distinct, time-dependent effects on each milk component.					
32475667	13	76	theme	T	1992:1992	arg1	cows					1994:1997	T cows	1992:1997	T cows	1992:1997	Plasma antioxidant potential and glucose concentration were lower in T cows than in C cows at 6 or 12 h, respectively, although neither variable remained different at 24 h.					
32475667	5	77	theme	saline	909:914	arg1	mL					903:904	only 10 mL	895:904	only 10 mL of saline (T-S)	895:920	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	11	78	theme	Lactose	1674:1680	arg1	concentration					1682:1694	Lactose concentration	1674:1694	Lactose concentration	1674:1694	Lactose concentration showed a local effect at 6 h, being lower in T-L than in T-S, and then a systemic effect at 12 h, being lower in both T-L and T-S than C quarters.					
32475667	15	79	theme	production	2375:2384	arg1	inhibition					2356:2365	systemic inhibition	2347:2365	systemic inhibition of milk production	2347:2384	Depending on the component, effects were local, systemic, or both, suggesting involvement of multiple different mediators that collectively result in systemic inhibition of milk production.					
32475667	5	80	theme	LPS	698:700	arg1	treatment					702:710	LPS treatment	698:710	LPS treatment (T)	698:714	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	5	80	theme	LPS	698:700	arg1	T					713:713	T	713:713	T	713:713	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	9	81	theme	fat	1405:1407	arg1	concentration					1409:1421	foremilk fat concentration	1396:1421	foremilk fat concentration	1396:1421	For T cows, systemic effects were observed by 3 h post-infusion as rectal temperature was elevated and foremilk fat concentration was reduced in both T-L and T-S.					
32475667	1	82	theme	mammary	158:164	arg1	gland					166:170	the bovine mammary gland	147:170	the bovine mammary gland	147:170	Each quarter of the bovine mammary gland is an anatomically and functionally distinct gland.					
32475667	8	83	theme	milk	1201:1204	arg1	yield					1206:1210	average milk yield	1193:1210	average milk yield in T-L and T-S quarters	1193:1234	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	2	84	dep	adjacent	280:287	arg1	uninfected					290:299	uninfected	290:299	uninfected	290:299	However, mastitis in one quarter may affect function of adjacent, uninfected glands.					
32475667	0	85	theme	bovine	108:113	arg1	glands					123:128	lactating bovine mammary glands	98:128	lactating bovine mammary glands	98:128	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	5	86	theme	saline	845:850	arg1	mL					839:840	10 mL	836:840	10 mL of saline (T-L)	836:856	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	13	87	theme	glucose	1956:1962	arg1	concentration					1964:1976	glucose concentration	1956:1976	glucose concentration	1956:1976	Plasma antioxidant potential and glucose concentration were lower in T cows than in C cows at 6 or 12 h, respectively, although neither variable remained different at 24 h.					
32475667	4	88	theme	cow-level	608:616	arg1	SCC					638:640	SCC	638:640	SCC	638:640	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	4	88	theme	cow-level	608:616	arg1	count					631:635	cow-level somatic cell count	608:635	cow-level somatic cell count (SCC)	608:641	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	3	89	theme	intramammary	435:446	arg1	challenge					473:481	intramammary lipopolysaccharide (LPS) challenge	435:481	intramammary lipopolysaccharide (LPS) challenge	435:481	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	0	90	theme	lipopolysaccharide	13:30	arg1	infusion					32:39	Intramammary lipopolysaccharide infusion	0:39	Intramammary lipopolysaccharide infusion	0:39	Intramammary lipopolysaccharide infusion induces local and systemic effects on milk components in lactating bovine mammary glands.					
32475667	2	91	from	mastitis	233:240	arg1	quarter					249:255	one quarter	245:255	one quarter	245:255	However, mastitis in one quarter may affect function of adjacent, uninfected glands.					
32475667	15	92	theme	mediators	2309:2317	arg1	involvement					2275:2285	involvement	2275:2285	involvement of multiple different mediators that collectively result in systemic inhibition of milk production	2275:2384	Depending on the component, effects were local, systemic, or both, suggesting involvement of multiple different mediators that collectively result in systemic inhibition of milk production.					
32475667	3	93	theme	gland	426:430	arg1	responses					401:409	early responses	395:409	early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge	395:481	To investigate the mechanisms and potential mediators of these effects, we quantified early responses of the mammary gland to intramammary lipopolysaccharide (LPS) challenge, distinguishing between local and systemic effects.					
32475667	12	94	from	changes	1860:1866	arg1	milk					1871:1874	milk	1871:1874	milk	1871:1874	Concomitant with changes in milk, systemic effects were also observed in blood.					
32475667	13	95	theme	antioxidant	1930:1940	arg1	potential					1942:1950	Plasma antioxidant potential	1923:1950	Plasma antioxidant potential	1923:1950	Plasma antioxidant potential and glucose concentration were lower in T cows than in C cows at 6 or 12 h, respectively, although neither variable remained different at 24 h.					
32475667	7	96	theme	foremilk	1083:1090	arg1	samples					1092:1098	foremilk samples	1083:1098	foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions	1083:1181	Cows were quarter milked twice daily, with foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions.					
32475667	7	96	theme	foremilk	1083:1090	arg1	mL					1105:1106	∼30 mL	1101:1106	∼30 mL	1101:1106	Cows were quarter milked twice daily, with foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions.					
32475667	10	97	theme	total	1505:1509	arg1	protein					1511:1517	total protein	1505:1517	total protein	1505:1517	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	4	98	from	cows	551:554	arg1	milk					569:572	milk	569:572	milk	569:572	Ten multiparous cows over 70 d in milk were blocked into pairs by breed, cow-level somatic cell count (SCC), and milk yield.					
32475667	10	99	theme	l-lactate	1491:1499	arg1	SCC					1465:1467	SCC	1465:1467	SCC	1465:1467	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	10	99	theme	l-lactate	1491:1499	arg1	concentrations					1473:1486	concentrations	1473:1486	concentrations of l-lactate and total protein in foremilk	1473:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	7	100	dep	mL	1105:1106	arg1	quarters					1115:1122	front quarters	1109:1122	front quarters	1109:1122	Cows were quarter milked twice daily, with foremilk samples (∼30 mL, front quarters) taken at -24, 0, 3, 6, 12, and 24 h relative to infusions.					
32475667	5	101	dep	treatment	702:710	arg1	such					716:719	such	716:719	such	716:719	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
32475667	8	102	theme	pre-infusion	1258:1269	arg1	levels					1271:1276	pre-infusion levels	1258:1276	pre-infusion levels	1258:1276	At 24 h, average milk yield in T-L and T-S quarters fell to 23 and 32% of pre-infusion levels, respectively.					
32475667	10	103	from	l-lactate	1491:1499	arg1	foremilk					1522:1529	foremilk	1522:1529	foremilk	1522:1529	However, SCC and concentrations of l-lactate and total protein in foremilk indicated a local response to LPS: protein was transiently higher at 3 h, whereas SCC and lactate were higher at 6 h in T-L compared with T-S.					
32475667	5	104	theme	contralateral	863:875	arg1	quarters					877:884	the contralateral quarters	859:884	the contralateral quarters	859:884	Within block, one cow was assigned to LPS treatment (T) such that both the front and the rear quarter of a randomly selected udder half received an infusion of 50 µg of LPS in 10 mL of saline (T-L); the contralateral quarters received only 10 mL of saline (T-S).					
33289538	2	0	theme	composite	626:634	arg1	hydrogel					636:643	a novel MPDA@GO/CNF composite hydrogel	606:643	a novel MPDA@GO/CNF composite hydrogel for controllable drug release	606:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	7	1	theme	GO/CNF	1217:1222	arg1	hydrogel					1234:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	3	2	theme	loading	717:723	arg1	ratio					725:729	a high drug loading ratio	705:729	a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH)	705:781	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	3	2	theme	loading	717:723	arg1	%					744:744	up to 35 wt %	732:744	up to 35 wt %	732:744	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	13	3	theme	physical	2288:2295	arg1	therapies					2297:2305	chemical and physical therapies	2275:2305	chemical and physical therapies	2275:2305	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	1	4	theme	sustained	292:300	arg1	abilities					315:323	sustained drug release abilities	292:323	sustained drug release abilities with low burst release	292:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	4	theme	sustained	292:300	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	10	5	theme	anomalous	1791:1799	arg1	mechanism					1743:1751	the drug release mechanism	1726:1751	the drug release mechanism of TH from the composite hydrogel	1726:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	10	5	theme	anomalous	1791:1799	arg1	transport					1801:1809	anomalous transport	1791:1809	anomalous transport	1791:1809	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	2	6	theme	@	618:618	arg1	hydrogel					636:643	a novel MPDA@GO/CNF composite hydrogel	606:643	a novel MPDA@GO/CNF composite hydrogel for controllable drug release	606:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	3	7	theme	wt	741:742	arg1	ratio					725:729	a high drug loading ratio	705:729	a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH)	705:781	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	3	7	theme	wt	741:742	arg1	%					744:744	up to 35 wt %	732:744	up to 35 wt %	732:744	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	1	8	theme	release	307:313	arg1	abilities					315:323	sustained drug release abilities	292:323	sustained drug release abilities with low burst release	292:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	8	theme	release	307:313	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	2	9	theme	drug	662:665	arg1	release					667:673	controllable drug release	649:673	controllable drug release	649:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	1	10	theme	intelligent	190:200	arg1	hydrogels					222:230	intelligent and multifunctional hydrogels	190:230	intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility	190:394	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	4	11	theme	release	853:859	arg1	time					861:864	the drug release time	844:864	the drug release time	844:864	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	3	12	theme	tetracycline	751:762	arg1	TH					779:780	TH	779:780	TH	779:780	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	3	12	theme	tetracycline	751:762	arg1	hydrochloride					764:776	tetracycline hydrochloride	751:776	tetracycline hydrochloride (TH)	751:781	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	10	13	theme	drug	1730:1733	arg1	mechanism					1743:1751	the drug release mechanism	1726:1751	the drug release mechanism of TH from the composite hydrogel	1726:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	10	13	theme	drug	1730:1733	arg1	transport					1801:1809	anomalous transport	1791:1809	anomalous transport	1791:1809	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	4	14	used	used	791:794	arg2	GO					784:785	GO	784:785	GO	784:785	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	1	15	theme	multifunctional	206:220	arg1	hydrogels					222:230	intelligent and multifunctional hydrogels	190:230	intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility	190:394	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	4	16	theme	hydrogel	920:927	arg1	strength					895:902	the physical strength	882:902	the physical strength of the obtained hydrogel	882:927	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	6	17	dep	@	1178:1178	arg1	GO					1179:1180	GO	1179:1180	GO	1179:1180	Drug release experiments demonstrated that burst release behavior was significantly reduced by adding MPDA@GO.					
33289538	0	18	theme	Drug	134:137	arg1	Release					139:145	Controllable Drug Release	121:145	Controllable Drug Release	121:145	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	5	19	theme	CNF	1058:1060	arg1	hydrogel					1062:1069	the pure CNF hydrogel	1049:1069	the pure CNF hydrogel	1049:1069	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	6	20	theme	release	1077:1083	arg1	experiments					1085:1095	Drug release experiments	1072:1095	Drug release experiments	1072:1095	Drug release experiments demonstrated that burst release behavior was significantly reduced by adding MPDA@GO.					
33289538	8	21	theme	composite	1415:1423	arg1	hydrogel					1425:1432	the composite hydrogel	1411:1432	the composite hydrogel	1411:1432	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	13	22	theme	encapsulation	2082:2094	arg1	structure					2096:2104	an encapsulation structure	2079:2104	an encapsulation structure for controllable drug release and toxicity shielding of GO	2079:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	10	23	theme	TH	1756:1757	arg1	mechanism					1743:1751	the drug release mechanism	1726:1751	the drug release mechanism of TH from the composite hydrogel	1726:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	10	23	theme	TH	1756:1757	arg1	transport					1801:1809	anomalous transport	1791:1809	anomalous transport	1791:1809	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	13	24	theme	potential	2248:2256	arg1	applications					2258:2269	potential applications	2248:2269	potential applications for chemical and physical therapies	2248:2305	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	2	25	theme	novel	608:612	arg1	hydrogel					636:643	a novel MPDA@GO/CNF composite hydrogel	606:643	a novel MPDA@GO/CNF composite hydrogel for controllable drug release	606:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	13	26	theme	novel	2037:2041	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	26	theme	novel	2037:2041	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	2	27	attach	cross-linked	544:555	arg3	hydrogel					587:594	cellulose nanofibril (CNF) hydrogel	560:594	cellulose nanofibril (CNF) hydrogel	560:594	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	13	28	theme	delivery	2215:2222	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	28	theme	delivery	2215:2222	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	7	29	theme	CNF	1328:1330	arg1	hydrogel					1332:1339	pure CNF hydrogel	1323:1339	pure CNF hydrogel	1323:1339	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	13	30	theme	MPDA	2043:2046	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	30	theme	MPDA	2043:2046	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	7	31	theme	@	1216:1216	arg1	hydrogel					1234:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	12	32	theme	CNF	2007:2009	arg1	hydrogel					2011:2018	the CNF hydrogel	2003:2018	the CNF hydrogel	2003:2018	In particular, the toxicity of GO was well shielded by the CNF hydrogel.					
33289538	13	33	theme	controlled	2199:2208	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	33	theme	controlled	2199:2208	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	2	34	theme	nanofibril	570:579	arg1	hydrogel					587:594	cellulose nanofibril (CNF) hydrogel	560:594	cellulose nanofibril (CNF) hydrogel	560:594	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	0	35	theme	Encapsulation	93:105	arg1	Structure					107:115	an Encapsulation Structure	90:115	an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding	90:168	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	2	36	dep	oxide	517:521	arg1	GO					524:525	GO	524:525	GO	524:525	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	5	37	theme	MPDA	975:978	arg1	hydrogel					997:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	11	38	theme	GO/CNF	1854:1859	arg1	hydrogel					1871:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	1	39	from	field	435:439	arg1	desirable					406:414	desirable	406:414	desirable	406:414	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	39	from	field	435:439	arg1	development					175:185	The development	171:185	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility	171:394	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	13	40	used	used	2174:2177	arg2	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	40	used	used	2174:2177	arg2	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	5	41	theme	GO/CNF	980:985	arg1	hydrogel					997:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	0	42	theme	Hydrogel	76:83	arg1	Construction					0:11	Construction	0:11	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.	0:169	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	11	43	theme	MPDA	1849:1852	arg1	hydrogel					1871:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	13	44	theme	@	2047:2047	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	44	theme	@	2047:2047	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	45	with	hydrogel	2065:2072	arg1	structure					2096:2104	an encapsulation structure	2079:2104	an encapsulation structure for controllable drug release and toxicity shielding of GO	2079:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	11	46	theme	cytotoxicity	1929:1940	arg1	test					1942:1945	an in vitro cytotoxicity test	1917:1945	an in vitro cytotoxicity test	1917:1945	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	11	47	theme	good	1887:1890	arg1	biocompatibility					1892:1907	good biocompatibility	1887:1907	good biocompatibility through an in vitro cytotoxicity test	1887:1945	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	1	48	from	desirable	406:414	arg1	field					435:439	the biomaterial field	419:439	the biomaterial field	419:439	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	49	theme	low	330:332	arg1	release					340:346	low burst release	330:346	low burst release	330:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	10	50	theme	release	1617:1623	arg1	kinetics					1625:1632	The drug release kinetics	1608:1632	The drug release kinetics of the as-prepared composite hydrogel	1608:1670	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	4	51	theme	MPDA	811:814	arg1	nanoparticles					816:828	MPDA nanoparticles	811:828	MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel	811:927	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	2	52	theme	mesoporous	450:459	arg1	nanoparticles					481:493	mesoporous polydopamine (MPDA) nanoparticles	450:493	mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO)	450:526	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	0	53	theme	Polydopamine	29:40	arg1	Hydrogel					76:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	13	54	theme	toxicity	2140:2147	arg1	shielding					2149:2157	toxicity shielding	2140:2157	toxicity shielding of GO	2140:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	5	55	theme	greater	1021:1027	arg1	strength					945:952	The mechanical strength	930:952	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel	930:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	5	55	theme	greater	1021:1027	arg1	times					1015:1019	five times	1010:1019	five times greater compared to that of the pure CNF hydrogel	1010:1069	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	7	56	theme	longer	1269:1274	arg1	times					1249:1253	3 times	1247:1253	3 times	1247:1253	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	7	56	theme	longer	1269:1274	arg1	time					1200:1203	The drug release time	1183:1203	The drug release time of the MPDA@GO/CNF composite hydrogel	1183:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	2	57	theme	graphene	508:515	arg1	oxide					517:521	graphene oxide	508:521	graphene oxide (GO)	508:526	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	0	58	theme	Composite	66:74	arg1	Hydrogel					76:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	8	59	theme	drug	1385:1388	arg1	behaviors					1398:1406	The sustained and controlled drug release behaviors	1356:1406	The sustained and controlled drug release behaviors of the composite hydrogel	1356:1432	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	8	59	theme	drug	1385:1388	arg1	dependent					1446:1454	dependent	1446:1454	dependent	1446:1454	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	10	60	theme	hydrogel	1663:1670	arg1	kinetics					1625:1632	The drug release kinetics	1608:1632	The drug release kinetics of the as-prepared composite hydrogel	1608:1670	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	0	61	theme	GO/Cellulose	42:53	arg1	Hydrogel					76:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	1	62	theme	antibacterial	349:361	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	62	theme	antibacterial	349:361	arg1	properties					363:372	antibacterial properties	349:372	antibacterial properties	349:372	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	13	63	contain	have	2243:2246	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	63	contain	have	2243:2246	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	63	contain	have	2243:2246	arg2	applications					2258:2269	potential applications	2248:2269	potential applications for chemical and physical therapies	2248:2305	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	3	64	theme	drug	712:715	arg1	ratio					725:729	a high drug loading ratio	705:729	a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH)	705:781	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	3	64	theme	drug	712:715	arg1	%					744:744	up to 35 wt %	732:744	up to 35 wt %	732:744	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	0	65	with	Construction	0:11	arg1	Structure					107:115	an Encapsulation Structure	90:115	an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding	90:168	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	10	66	theme	as-prepared	1641:1651	arg1	hydrogel					1663:1670	the as-prepared composite hydrogel	1637:1670	the as-prepared composite hydrogel	1637:1670	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	13	67	theme	controllable	2110:2121	arg1	release					2128:2134	controllable drug release	2110:2134	controllable drug release	2110:2134	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	5	68	theme	hydrogel	997:1004	arg1	strength					945:952	The mechanical strength	930:952	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel	930:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	5	68	theme	hydrogel	997:1004	arg1	times					1015:1019	five times	1010:1019	five times greater compared to that of the pure CNF hydrogel	1010:1069	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	6	69	theme	burst	1115:1119	arg1	behavior					1129:1136	burst release behavior	1115:1136	burst release behavior	1115:1136	Drug release experiments demonstrated that burst release behavior was significantly reduced by adding MPDA@GO.					
33289538	10	70	theme	drug	1612:1615	arg1	kinetics					1625:1632	The drug release kinetics	1608:1632	The drug release kinetics of the as-prepared composite hydrogel	1608:1670	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	1	71	theme	mechanical	269:278	arg1	properties					280:289	good mechanical properties	264:289	good mechanical properties	264:289	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	71	theme	mechanical	269:278	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	9	72	theme	pH	1591:1592	arg1	change					1600:1605	pH value change	1591:1605	pH value change	1591:1605	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	2	73	theme	GO/CNF	619:624	arg1	hydrogel					636:643	a novel MPDA@GO/CNF composite hydrogel	606:643	a novel MPDA@GO/CNF composite hydrogel for controllable drug release	606:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	7	74	theme	composite	1224:1232	arg1	hydrogel					1234:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	9	75	theme	value	1594:1598	arg1	change					1600:1605	pH value change	1591:1605	pH value change	1591:1605	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	1	76	theme	drug	302:305	arg1	abilities					315:323	sustained drug release abilities	292:323	sustained drug release abilities with low burst release	292:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	76	theme	drug	302:305	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	2	77	theme	MPDA	614:617	arg1	hydrogel					636:643	a novel MPDA@GO/CNF composite hydrogel	606:643	a novel MPDA@GO/CNF composite hydrogel for controllable drug release	606:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	10	78	theme	Korsmeyer-Peppas	1698:1713	arg1	model					1715:1719	the Korsmeyer-Peppas model	1694:1719	the Korsmeyer-Peppas model	1694:1719	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	13	79	theme	chemical	2275:2282	arg1	therapies					2297:2305	chemical and physical therapies	2275:2305	chemical and physical therapies	2275:2305	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	2	80	theme	controllable	649:660	arg1	release					667:673	controllable drug release	649:673	controllable drug release	649:673	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	6	81	theme	MPDA	1174:1177	arg1	@					1178:1178	MPDA@GO	1174:1180	MPDA@GO	1174:1180	Drug release experiments demonstrated that burst release behavior was significantly reduced by adding MPDA@GO.					
33289538	10	82	theme	release	1735:1741	arg1	mechanism					1743:1751	the drug release mechanism	1726:1751	the drug release mechanism of TH from the composite hydrogel	1726:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	10	82	theme	release	1735:1741	arg1	transport					1801:1809	anomalous transport	1791:1809	anomalous transport	1791:1809	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	11	83	dep	in	1920:1921	arg1	vitro					1923:1927	vitro	1923:1927	vitro	1923:1927	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	4	84	theme	drug	848:851	arg1	time					861:864	the drug release time	844:864	the drug release time	844:864	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	1	85	with	abilities	315:323	arg1	release					340:346	low burst release	330:346	low burst release	330:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	86	theme	hydrogels	222:230	arg1	desirable					406:414	desirable	406:414	desirable	406:414	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	86	theme	hydrogels	222:230	arg1	development					175:185	The development	171:185	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility	171:394	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	4	87	theme	obtained	911:918	arg1	hydrogel					920:927	the obtained hydrogel	907:927	the obtained hydrogel	907:927	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	8	88	theme	hydrogel	1425:1432	arg1	behaviors					1398:1406	The sustained and controlled drug release behaviors	1356:1406	The sustained and controlled drug release behaviors of the composite hydrogel	1356:1432	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	8	88	theme	hydrogel	1425:1432	arg1	dependent					1446:1454	dependent	1446:1454	dependent	1446:1454	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	0	89	theme	Toxicity	151:158	arg1	Shielding					160:168	Toxicity Shielding	151:168	Toxicity Shielding	151:168	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	13	90	theme	composite	2055:2063	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	90	theme	composite	2055:2063	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	11	91	theme	in	1920:1921	arg1	test					1942:1945	an in vitro cytotoxicity test	1917:1945	an in vitro cytotoxicity test	1917:1945	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	1	92	theme	photothermal	239:250	arg1	abilities					315:323	sustained drug release abilities	292:323	sustained drug release abilities with low burst release	292:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	92	theme	photothermal	239:250	arg1	properties					363:372	antibacterial properties	349:372	antibacterial properties	349:372	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	92	theme	photothermal	239:250	arg1	properties					280:289	good mechanical properties	264:289	good mechanical properties	264:289	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	92	theme	photothermal	239:250	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	13	93	theme	drug	2210:2213	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	93	theme	drug	2210:2213	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	4	94	theme	physical	886:893	arg1	strength					895:902	the physical strength	882:902	the physical strength of the obtained hydrogel	882:927	GO was used to encapsulate MPDA nanoparticles for extending the drug release time and reinforcing the physical strength of the obtained hydrogel.					
33289538	9	95	theme	light	1569:1573	arg1	irradiation					1575:1585	near-infrared (NIR) light irradiation	1549:1585	near-infrared (NIR) light irradiation	1549:1585	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	7	96	theme	MPDA	1212:1215	arg1	hydrogel					1234:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	the MPDA@GO/CNF composite hydrogel	1208:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	5	97	theme	pure	1053:1056	arg1	hydrogel					1062:1069	the pure CNF hydrogel	1049:1069	the pure CNF hydrogel	1049:1069	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	10	98	theme	composite	1768:1776	arg1	hydrogel					1778:1785	the composite hydrogel	1764:1785	the composite hydrogel	1764:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	8	99	theme	MPDA	1477:1480	arg1	proportion					1463:1472	the proportion	1459:1472	the proportion of MPDA and GO	1459:1487	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	7	100	theme	pure	1323:1326	arg1	hydrogel					1332:1339	pure CNF hydrogel	1323:1339	pure CNF hydrogel	1323:1339	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	13	101	theme	promising	2189:2197	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	101	theme	promising	2189:2197	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	8	102	theme	GO	1486:1487	arg1	proportion					1463:1472	the proportion	1459:1472	the proportion of MPDA and GO	1459:1487	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	1	103	theme	biomaterial	423:433	arg1	field					435:439	the biomaterial field	419:439	the biomaterial field	419:439	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	7	104	theme	release	1192:1198	arg1	times					1249:1253	3 times	1247:1253	3 times	1247:1253	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	7	104	theme	release	1192:1198	arg1	time					1200:1203	The drug release time	1183:1203	The drug release time of the MPDA@GO/CNF composite hydrogel	1183:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	5	105	theme	as-fabricated	961:973	arg1	hydrogel					997:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	2	106	theme	cellulose	560:568	arg1	CNF					582:584	CNF	582:584	CNF	582:584	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	2	106	theme	cellulose	560:568	arg1	nanofibril					570:579	cellulose nanofibril	560:579	cellulose nanofibril (CNF) hydrogel	560:594	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	0	107	theme	Controllable	121:132	arg1	Release					139:145	Controllable Drug Release	121:145	Controllable Drug Release	121:145	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	5	108	theme	@	979:979	arg1	hydrogel					997:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	10	109	from	mechanism	1743:1751	arg1	hydrogel					1778:1785	the composite hydrogel	1764:1785	the composite hydrogel	1764:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	6	110	theme	Drug	1072:1075	arg1	experiments					1085:1095	Drug release experiments	1072:1095	Drug release experiments	1072:1095	Drug release experiments demonstrated that burst release behavior was significantly reduced by adding MPDA@GO.					
33289538	13	111	theme	drug	2123:2126	arg1	release					2128:2134	controllable drug release	2110:2134	controllable drug release	2110:2134	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	11	112	theme	composite	1861:1869	arg1	hydrogel					1871:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	5	113	theme	mechanical	934:943	arg1	strength					945:952	The mechanical strength	930:952	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel	930:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	5	113	theme	mechanical	934:943	arg1	times					1015:1019	five times	1010:1019	five times greater compared to that of the pure CNF hydrogel	1010:1069	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	11	114	theme	@	1853:1853	arg1	hydrogel					1871:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
33289538	0	115	theme	Mesoporous	18:27	arg1	Hydrogel					76:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	2	116	theme	MPDA	475:478	arg1	nanoparticles					481:493	mesoporous polydopamine (MPDA) nanoparticles	450:493	mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO)	450:526	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	9	117	theme	drug	1512:1515	arg1	release					1517:1523	drug release	1512:1523	drug release	1512:1523	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	3	118	theme	MPDA	676:679	arg1	nanoparticles					681:693	MPDA nanoparticles	676:693	MPDA nanoparticles	676:693	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	2	119	theme	polydopamine	461:472	arg1	nanoparticles					481:493	mesoporous polydopamine (MPDA) nanoparticles	450:493	mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO)	450:526	Herein, mesoporous polydopamine (MPDA) nanoparticles wrapped with graphene oxide (GO) were physically cross-linked in cellulose nanofibril (CNF) hydrogel to obtain a novel MPDA@GO/CNF composite hydrogel for controllable drug release.					
33289538	0	120	theme	@	41:41	arg1	Hydrogel					76:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	1	121	theme	burst	334:338	arg1	release					340:346	low burst release	330:346	low burst release	330:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	9	122	theme	near-infrared	1549:1561	arg1	irradiation					1575:1585	near-infrared (NIR) light irradiation	1549:1585	near-infrared (NIR) light irradiation	1549:1585	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	10	123	from	hydrogel	1778:1785	arg1	mechanism					1743:1751	the drug release mechanism	1726:1751	the drug release mechanism of TH from the composite hydrogel	1726:1785	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	10	123	from	hydrogel	1778:1785	arg1	transport					1801:1809	anomalous transport	1791:1809	anomalous transport	1791:1809	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	7	124	theme	polydopamine/CNF	1293:1308	arg1	hydrogel					1310:1317	the polydopamine/CNF hydrogel	1289:1317	the polydopamine/CNF hydrogel	1289:1317	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	8	125	theme	sustained	1360:1368	arg1	behaviors					1398:1406	The sustained and controlled drug release behaviors	1356:1406	The sustained and controlled drug release behaviors of the composite hydrogel	1356:1432	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	8	125	theme	sustained	1360:1368	arg1	dependent					1446:1454	dependent	1446:1454	dependent	1446:1454	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	7	126	theme	drug	1187:1190	arg1	times					1249:1253	3 times	1247:1253	3 times	1247:1253	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	7	126	theme	drug	1187:1190	arg1	time					1200:1203	The drug release time	1183:1203	The drug release time of the MPDA@GO/CNF composite hydrogel	1183:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	0	127	theme	Nanofibril	55:64	arg1	Hydrogel					76:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel	16:83	Construction of a Mesoporous Polydopamine@GO/Cellulose Nanofibril Composite Hydrogel with an Encapsulation Structure for Controllable Drug Release and Toxicity Shielding.					
33289538	3	128	dep	35	738:739	arg1	to					735:736	to	735:736	to	735:736	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	9	129	theme	NIR	1564:1566	arg1	irradiation					1575:1585	near-infrared (NIR) light irradiation	1549:1585	near-infrared (NIR) light irradiation	1549:1585	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	3	130	theme	high	707:710	arg1	ratio					725:729	a high drug loading ratio	705:729	a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH)	705:781	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	3	130	theme	high	707:710	arg1	%					744:744	up to 35 wt %	732:744	up to 35 wt %	732:744	MPDA nanoparticles exhibited a high drug loading ratio (up to 35 wt %) for tetracycline hydrochloride (TH).					
33289538	8	131	theme	controlled	1374:1383	arg1	behaviors					1398:1406	The sustained and controlled drug release behaviors	1356:1406	The sustained and controlled drug release behaviors of the composite hydrogel	1356:1432	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	8	131	theme	controlled	1374:1383	arg1	dependent					1446:1454	dependent	1446:1454	dependent	1446:1454	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	10	132	theme	composite	1653:1661	arg1	hydrogel					1663:1670	the as-prepared composite hydrogel	1637:1670	the as-prepared composite hydrogel	1637:1670	The drug release kinetics of the as-prepared composite hydrogel was well described by the Korsmeyer-Peppas model, and the drug release mechanism of TH from the composite hydrogel was anomalous transport.					
33289538	13	133	theme	GO	2162:2163	arg1	release					2128:2134	controllable drug release	2110:2134	controllable drug release	2110:2134	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	133	theme	GO	2162:2163	arg1	shielding					2149:2157	toxicity shielding	2140:2157	toxicity shielding of GO	2140:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	134	theme	GO/CNF	2048:2053	arg1	carrier					2224:2230	a very promising controlled drug delivery carrier	2182:2230	a very promising controlled drug delivery carrier	2182:2230	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	13	134	theme	GO/CNF	2048:2053	arg1	hydrogel					2065:2072	this novel MPDA@GO/CNF composite hydrogel	2032:2072	this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO	2032:2163	Therefore, this novel MPDA@GO/CNF composite hydrogel with an encapsulation structure for controllable drug release and toxicity shielding of GO could be used as a very promising controlled drug delivery carrier, which may have potential applications for chemical and physical therapies.					
33289538	5	135	theme	composite	987:995	arg1	hydrogel					997:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	the as-fabricated MPDA@GO/CNF composite hydrogel	957:1004	The mechanical strength of the as-fabricated MPDA@GO/CNF composite hydrogel was five times greater compared to that of the pure CNF hydrogel.					
33289538	6	136	theme	release	1121:1127	arg1	behavior					1129:1136	burst release behavior	1115:1136	burst release behavior	1115:1136	Drug release experiments demonstrated that burst release behavior was significantly reduced by adding MPDA@GO.					
33289538	9	137	theme	release	1517:1523	arg1	rate					1504:1507	the rate	1500:1507	the rate of drug release	1500:1523	Moreover, the rate of drug release could be accelerated by near-infrared (NIR) light irradiation and pH value change.					
33289538	1	138	contain	having	232:237	arg2	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	138	contain	having	232:237	arg2	abilities					315:323	sustained drug release abilities	292:323	sustained drug release abilities with low burst release	292:346	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	138	contain	having	232:237	arg1	hydrogels					222:230	intelligent and multifunctional hydrogels	190:230	intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility	190:394	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	138	contain	having	232:237	arg2	biocompatibility					379:394	biocompatibility	379:394	biocompatibility	379:394	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	138	contain	having	232:237	arg2	properties					363:372	antibacterial properties	349:372	antibacterial properties	349:372	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	138	contain	having	232:237	arg2	properties					280:289	good mechanical properties	264:289	good mechanical properties	264:289	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	8	139	theme	release	1390:1396	arg1	behaviors					1398:1406	The sustained and controlled drug release behaviors	1356:1406	The sustained and controlled drug release behaviors of the composite hydrogel	1356:1432	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	8	139	theme	release	1390:1396	arg1	dependent					1446:1454	dependent	1446:1454	dependent	1446:1454	The sustained and controlled drug release behaviors of the composite hydrogel were highly dependent on the proportion of MPDA and GO.					
33289538	1	140	theme	good	264:267	arg1	properties					280:289	good mechanical properties	264:289	good mechanical properties	264:289	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	1	140	theme	good	264:267	arg1	properties					252:261	photothermal properties	239:261	photothermal properties	239:261	The development of intelligent and multifunctional hydrogels having photothermal properties, good mechanical properties, sustained drug release abilities with low burst release, antibacterial properties, and biocompatibility is highly desirable in the biomaterial field.					
33289538	12	141	theme	GO	1979:1980	arg1	toxicity					1967:1974	the toxicity	1963:1974	the toxicity of GO	1963:1980	In particular, the toxicity of GO was well shielded by the CNF hydrogel.					
33289538	7	142	theme	hydrogel	1234:1241	arg1	times					1249:1253	3 times	1247:1253	3 times	1247:1253	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	7	142	theme	hydrogel	1234:1241	arg1	time					1200:1203	The drug release time	1183:1203	The drug release time of the MPDA@GO/CNF composite hydrogel	1183:1241	The drug release time of the MPDA@GO/CNF composite hydrogel was 3 times and 7.2 times longer than that of the polydopamine/CNF hydrogel and pure CNF hydrogel, respectively.					
33289538	11	143	theme	designed	1840:1847	arg1	hydrogel					1871:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	this carefully designed MPDA@GO/CNF composite hydrogel	1825:1878	Importantly, this carefully designed MPDA@GO/CNF composite hydrogel showed good biocompatibility through an in vitro cytotoxicity test.					
34725434	7	0	theme	first	1149:1153	arg1	product					1155:1161	a first product	1147:1161	a first product cleared as an aid for weight management in the US and Europe	1147:1222	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	3	1	theme	simulated	524:532	arg1	environment					551:561	a simulated gastrointestinal environment	522:561	a simulated gastrointestinal environment	522:561	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	6	2	theme	similar	934:940	arg1	levels					927:932	elasticity levels	916:932	elasticity levels similar to raw vegetables	916:958	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	3	3	theme	gastrointestinal	534:549	arg1	environment					551:561	a simulated gastrointestinal environment	522:561	a simulated gastrointestinal environment	522:561	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	3	4	from	impact	576:581	arg1	tissue					590:595	gut tissue	586:595	gut tissue	586:595	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	2	5	dep	composition	394:404	arg1	the					390:392	the	390:392	the	390:392	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	6	theme	hydrogel	350:357	arg1	platform					368:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	6	7	theme	elasticity	916:925	arg1	levels					927:932	elasticity levels	916:932	elasticity levels similar to raw vegetables	916:958	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	3	8	theme	gut	586:588	arg1	tissue					590:595	gut tissue	586:595	gut tissue	586:595	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	4	9	theme	Functional	656:665	arg1	reference					706:714	reference	706:714	reference	706:714	Functional fibers and raw vegetables were used as reference.					
34725434	4	9	theme	Functional	656:665	arg1	fibers					667:672	Functional fibers	656:672	Functional fibers	656:672	Functional fibers and raw vegetables were used as reference.					
34725434	4	9	theme	Functional	656:665	arg1	vegetables					682:691	raw vegetables	678:691	raw vegetables	678:691	Functional fibers and raw vegetables were used as reference.					
34725434	5	10	theme	higher	758:763	arg1	elasticity					765:774	orders of magnitude higher elasticity	738:774	orders of magnitude higher elasticity	738:774	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34725434	5	11	theme	tested	856:861	arg1	vegetables					867:876	the tested raw vegetables	852:876	the tested raw vegetables	852:876	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34725434	6	12	theme	gut	1009:1011	arg1	tissue					1013:1018	the gut tissue	1005:1018	the gut tissue	1005:1018	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	7	13	from	management	1192:1201	arg1	Europe					1217:1222	Europe	1217:1222	Europe	1217:1222	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	7	13	from	management	1192:1201	arg1	US					1210:1211	US	1210:1211	US	1210:1211	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	0	14	theme	Biomimetic	0:9	arg1	superabsorbent					27:40	Biomimetic cellulose-based superabsorbent	0:40	Biomimetic cellulose-based superabsorbent	0:40	Biomimetic cellulose-based superabsorbent hydrogels for treating obesity.					
34725434	2	15	theme	mechanical	410:419	arg1	properties					421:430	mechanical properties	410:430	mechanical properties	410:430	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	0	16	theme	cellulose-based	11:25	arg1	superabsorbent					27:40	Biomimetic cellulose-based superabsorbent	0:40	Biomimetic cellulose-based superabsorbent	0:40	Biomimetic cellulose-based superabsorbent hydrogels for treating obesity.					
34725434	6	17	theme	biomimetic	892:901	arg1	CB-SAHs					903:909	the biomimetic CB-SAHs	888:909	the biomimetic CB-SAHs with elasticity levels similar to raw vegetables	888:958	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	7	18	dep	an	1174:1175	arg1	aid					1177:1179	aid	1177:1179	aid	1177:1179	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	1	19	from	difficult	148:156	arg1	treatment					81:89	the treatment	77:89	the treatment of obesity	77:100	In the treatment of obesity, nutritional and behavioral modifications are difficult to implement and maintain.					
34725434	1	20	theme	obesity	94:100	arg1	treatment					81:89	the treatment	77:89	the treatment of obesity	77:100	In the treatment of obesity, nutritional and behavioral modifications are difficult to implement and maintain.					
34725434	3	21	dep	ex	620:621	arg1	vivo					623:626	vivo	623:626	vivo	623:626	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	2	22	theme	mechanobiological	456:472	arg1	therapy					474:480	a mechanobiological therapy	454:480	a mechanobiological therapy	454:480	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	7	23	theme	clinical	1124:1131	arg1	studies					1133:1139	clinical studies	1124:1139	clinical studies	1124:1139	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	2	24	theme	superabsorbent	335:348	arg1	platform					368:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	4	25	theme	raw	678:680	arg1	reference					706:714	reference	706:714	reference	706:714	Functional fibers and raw vegetables were used as reference.					
34725434	4	25	theme	raw	678:680	arg1	fibers					667:672	Functional fibers	656:672	Functional fibers	656:672	Functional fibers and raw vegetables were used as reference.					
34725434	4	25	theme	raw	678:680	arg1	vegetables					682:691	raw vegetables	678:691	raw vegetables	678:691	Functional fibers and raw vegetables were used as reference.					
34725434	5	26	theme	raw	863:865	arg1	vegetables					867:876	the tested raw vegetables	852:876	the tested raw vegetables	852:876	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34725434	3	27	theme	organ	628:632	arg1	model					649:653	an ex vivo organ culture (EVOC) model	617:653	an ex vivo organ culture (EVOC) model	617:653	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	4	28	used	used	698:701	arg2	vegetables					682:691	raw vegetables	678:691	raw vegetables	678:691	Functional fibers and raw vegetables were used as reference.					
34725434	4	28	used	used	698:701	arg2	reference					706:714	reference	706:714	reference	706:714	Functional fibers and raw vegetables were used as reference.					
34725434	4	28	used	used	698:701	arg2	fibers					667:672	Functional fibers	656:672	Functional fibers	656:672	Functional fibers and raw vegetables were used as reference.					
34725434	2	29	theme	cellulose-based	319:333	arg1	platform					368:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	3	30	theme	ex	620:621	arg1	model					649:653	an ex vivo organ culture (EVOC) model	617:653	an ex vivo organ culture (EVOC) model	617:653	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	3	31	theme	CB-SAHs	487:493	arg1	properties					495:504	The CB-SAHs properties	483:504	The CB-SAHs properties	483:504	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	7	32	theme	weight	1185:1190	arg1	management					1192:1201	weight management	1185:1201	weight management in the US and Europe	1185:1222	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	6	33	theme	EVOC	1027:1030	arg1	model					1032:1036	the EVOC model	1023:1036	the EVOC model	1023:1036	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	2	34	theme	novel	313:317	arg1	platform					368:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	35	theme	vegetables	439:448	arg1	composition					394:404	composition	394:404	composition	394:404	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	35	theme	vegetables	439:448	arg1	properties					421:430	mechanical properties	410:430	mechanical properties	410:430	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	5	36	theme	tested	797:802	arg1	fibers					815:820	the tested functional fibers	793:820	the tested functional fibers	793:820	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34725434	2	37	theme	vegetable	191:199	arg1	consumption					201:211	vegetable consumption	191:211	vegetable consumption	191:211	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	37	theme	vegetable	191:199	arg1	part					230:233	a fundamental part	216:233	a fundamental part of many dietary interventions and daily nutrient requirements	216:295	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	38	theme	interventions	251:263	arg1	consumption					201:211	vegetable consumption	191:211	vegetable consumption	191:211	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	38	theme	interventions	251:263	arg1	part					230:233	a fundamental part	216:233	a fundamental part of many dietary interventions and daily nutrient requirements	216:295	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	39	theme	raw	435:437	arg1	vegetables					439:448	raw vegetables	435:448	raw vegetables	435:448	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	5	40	theme	functional	804:813	arg1	fibers					815:820	the tested functional fibers	793:820	the tested functional fibers	793:820	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34725434	3	41	theme	EVOC	643:646	arg1	model					649:653	an ex vivo organ culture (EVOC) model	617:653	an ex vivo organ culture (EVOC) model	617:653	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	2	42	theme	dietary	243:249	arg1	interventions					251:263	many dietary interventions	238:263	many dietary interventions	238:263	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	7	43	with	advanced	1107:1114	arg1	product					1155:1161	a first product	1147:1161	a first product cleared as an aid for weight management in the US and Europe	1147:1222	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	2	44	theme	many	238:241	arg1	interventions					251:263	many dietary interventions	238:263	many dietary interventions	238:263	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	7	45	theme	Non-systemic	1039:1050	arg1	mechanotherapeutics					1057:1075	Non-systemic oral mechanotherapeutics	1039:1075	Non-systemic oral mechanotherapeutics based on this technology	1039:1100	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	1	46	theme	behavioral	119:128	arg1	modifications					130:142	nutritional and behavioral modifications	103:142	nutritional and behavioral modifications	103:142	In the treatment of obesity, nutritional and behavioral modifications are difficult to implement and maintain.					
34725434	7	47	dep	US	1210:1211	arg1	the					1206:1208	the	1206:1208	the	1206:1208	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	7	48	theme	oral	1052:1055	arg1	mechanotherapeutics					1057:1075	Non-systemic oral mechanotherapeutics	1039:1075	Non-systemic oral mechanotherapeutics based on this technology	1039:1100	Non-systemic oral mechanotherapeutics based on this technology were advanced through clinical studies, with a first product cleared as an aid for weight management in the US and Europe.					
34725434	2	49	theme	fundamental	218:228	arg1	consumption					201:211	vegetable consumption	191:211	vegetable consumption	191:211	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	49	theme	fundamental	218:228	arg1	part					230:233	a fundamental part	216:233	a fundamental part of many dietary interventions and daily nutrient requirements	216:295	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	50	theme	requirements	284:295	arg1	consumption					201:211	vegetable consumption	191:211	vegetable consumption	191:211	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	50	theme	requirements	284:295	arg1	part					230:233	a fundamental part	216:233	a fundamental part of many dietary interventions and daily nutrient requirements	216:295	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	2	51	theme	CB-SAH	360:365	arg1	platform					368:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform	311:375	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	1	52	theme	nutritional	103:113	arg1	modifications					130:142	nutritional and behavioral modifications	103:142	nutritional and behavioral modifications	103:142	In the treatment of obesity, nutritional and behavioral modifications are difficult to implement and maintain.					
34725434	2	53	theme	nutrient	275:282	arg1	requirements					284:295	daily nutrient requirements	269:295	daily nutrient requirements	269:295	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	5	54	dep	demonstrated	725:736	arg1	performed					831:839	performed	831:839	performed similar to the tested raw vegetables	831:876	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34725434	2	55	theme	daily	269:273	arg1	requirements					284:295	daily nutrient requirements	269:295	daily nutrient requirements	269:295	Since vegetable consumption is a fundamental part of many dietary interventions and daily nutrient requirements, we developed a novel cellulose-based superabsorbent hydrogel (CB-SAH) platform, inspired by the composition and mechanical properties of raw vegetables, as a mechanobiological therapy.					
34725434	6	56	with	CB-SAHs	903:909	arg1	levels					927:932	elasticity levels	916:932	elasticity levels similar to raw vegetables	916:958	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	6	57	theme	raw	945:947	arg1	vegetables					949:958	raw vegetables	945:958	raw vegetables	945:958	Notably, the biomimetic CB-SAHs with elasticity levels similar to raw vegetables showed benefits in preserving and regulating the gut tissue in the EVOC model.					
34725434	3	58	theme	culture	634:640	arg1	model					649:653	an ex vivo organ culture (EVOC) model	617:653	an ex vivo organ culture (EVOC) model	617:653	The CB-SAHs properties were studied in a simulated gastrointestinal environment, while their impact on gut tissue was investigated by an ex vivo organ culture (EVOC) model.					
34725434	1	59	from	treatment	81:89	arg1	difficult					148:156	difficult	148:156	difficult	148:156	In the treatment of obesity, nutritional and behavioral modifications are difficult to implement and maintain.					
34725434	5	60	theme	magnitude	748:756	arg1	orders					738:743	orders	738:743	orders of magnitude	738:756	CB-SAHs demonstrated orders of magnitude higher elasticity in comparison to the tested functional fibers, however performed similar to the tested raw vegetables.					
34948280	5	0	theme	flow	748:751	arg1	curves					753:758	flow curves	748:758	flow curves	748:758	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	8	1	dep	5.0	1170:1172	arg1	to					1167:1168	to	1167:1168	to	1167:1168	Various large-scale scaffolds were fabricated (up to 5.0 cm) using a pre-crosslinked hybrid.					
34948280	10	2	theme	proper	1539:1544	arg1	geometry					1546:1553	proper geometry	1539:1553	proper geometry	1539:1553	This pre-crosslinking-based rheological property controlling technique can open a new avenue for 3D bio-fabrication of scaffolds, ensuring proper geometry.					
34948280	6	3	theme	fabricated	976:985	arg1	scaffolds					987:995	the fabricated scaffolds	972:995	the fabricated scaffolds	972:995	The geometrical fidelity of the fabricated scaffolds was defined by printability and collapse tests.					
34948280	5	4	theme	curves	753:758	arg1	tests					735:739	rheological tests	723:739	rheological tests	723:739	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	4	theme	curves	753:758	arg1	tests					771:775	amplitude tests	761:775	amplitude tests	761:775	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	4	theme	curves	753:758	arg1	tests					809:813	three interval thixotropic tests	782:813	three interval thixotropic tests	782:813	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	4	theme	curves	753:758	arg1	curves					753:758	flow curves	748:758	flow curves	748:758	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	4	theme	curves	753:758	arg1	set					716:718	A set	714:718	A set	714:718	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	5	theme	recovery	905:912	arg1	rate					914:917	recovery rate	905:917	recovery rate of various compositions	905:941	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	9	6	theme	coli	1287:1290	arg1	growth					1265:1270	the growth	1261:1270	the growth of Escherichia coli	1261:1290	Scaffolds were assessed for the ability to support the growth of Escherichia coli using the Most Probable Number technique to quantify bacteria immediately after inoculation and 24 h later.					
34948280	3	7	theme	various	414:420	arg1	percentages					422:432	various percentages	414:432	various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer)	414:486	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	1	8	theme	shape	69:73	arg1	fidelity					75:82	shape fidelity	69:82	shape fidelity of 3D bio-printed scaffolds with soft biomaterials	69:133	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	2	9	theme	scaffolds	321:329	arg1	fidelity					294:301	the geometric fidelity	280:301	the geometric fidelity of 3D bio-printed scaffolds	280:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	3	10	dep	CaCl2	517:521	arg1	cross-linkers					543:555	physical cross-linkers	534:555	physical cross-linkers	534:555	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	6	11	dep	printability	1012:1023	arg1	tests					1038:1042	tests	1038:1042	tests	1038:1042	The geometrical fidelity of the fabricated scaffolds was defined by printability and collapse tests.					
34948280	2	12	theme	3D	306:307	arg1	scaffolds					321:329	3D bio-printed scaffolds	306:329	3D bio-printed scaffolds	306:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	3	13	theme	calcium	502:508	arg1	salts					510:514	different calcium salts	492:514	different calcium salts (CaCl2 and CaSO4, physical cross-linkers)	492:556	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	13	theme	calcium	502:508	arg1	CaSO4					527:531	CaSO4	527:531	CaSO4	527:531	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	13	theme	calcium	502:508	arg1	CaCl2					517:521	CaCl2	517:521	CaCl2	517:521	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	14	theme	post-printing	367:379	arg1	fidelity					387:394	post-printing shape fidelity	367:394	post-printing shape fidelity	367:394	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	10	15	theme	pre-crosslinking-based	1405:1426	arg1	property					1440:1447	This pre-crosslinking-based rheological property	1400:1447	This pre-crosslinking-based rheological property controlling technique	1400:1469	This pre-crosslinking-based rheological property controlling technique can open a new avenue for 3D bio-fabrication of scaffolds, ensuring proper geometry.					
34948280	8	16	dep	fabricated	1152:1161	arg1	cm					1174:1175	up to 5.0 cm	1164:1175	up to 5.0 cm	1164:1175	Various large-scale scaffolds were fabricated (up to 5.0 cm) using a pre-crosslinked hybrid.					
34948280	3	17	theme	physical	534:541	arg1	cross-linkers					543:555	physical cross-linkers	534:555	physical cross-linkers	534:555	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	5	18	theme	amplitude	761:769	arg1	tests					771:775	amplitude tests	761:775	amplitude tests	761:775	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	3	19	theme	shape	381:385	arg1	fidelity					387:394	post-printing shape fidelity	367:394	post-printing shape fidelity	367:394	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	20	theme	Carboxymethyl	437:449	arg1	enhancer					478:485	viscosity enhancer	468:485	viscosity enhancer	468:485	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	20	theme	Carboxymethyl	437:449	arg1	CMC					462:464	CMC	462:464	CMC	462:464	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	20	theme	Carboxymethyl	437:449	arg1	Cellulose					451:459	Carboxymethyl Cellulose	437:459	Carboxymethyl Cellulose (CMC) (viscosity enhancer)	437:486	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	10	21	theme	rheological	1428:1438	arg1	property					1440:1447	This pre-crosslinking-based rheological property	1400:1447	This pre-crosslinking-based rheological property controlling technique	1400:1469	This pre-crosslinking-based rheological property controlling technique can open a new avenue for 3D bio-fabrication of scaffolds, ensuring proper geometry.					
34948280	1	22	theme	3D	87:88	arg1	scaffolds					102:110	3D bio-printed scaffolds	87:110	3D bio-printed scaffolds	87:110	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	4	23	theme	Cellulose	679:687	arg1	content					645:651	The overall solid content	627:651	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC)	627:693	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC) was limited to 6%.					
34948280	9	24	theme	Probable	1307:1314	arg1	technique					1323:1331	the Most Probable Number technique	1298:1331	the Most Probable Number technique	1298:1331	Scaffolds were assessed for the ability to support the growth of Escherichia coli using the Most Probable Number technique to quantify bacteria immediately after inoculation and 24 h later.					
34948280	9	25	theme	Number	1316:1321	arg1	technique					1323:1331	the Most Probable Number technique	1298:1331	the Most Probable Number technique	1298:1331	Scaffolds were assessed for the ability to support the growth of Escherichia coli using the Most Probable Number technique to quantify bacteria immediately after inoculation and 24 h later.					
34948280	1	26	theme	bio-printed	90:100	arg1	scaffolds					102:110	3D bio-printed scaffolds	87:110	3D bio-printed scaffolds	87:110	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	0	27	theme	3D	0:1	arg1	Bio-Printability					3:18	3D Bio-Printability	0:18	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels	0:54	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels.					
34948280	6	28	theme	geometrical	948:958	arg1	fidelity					960:967	The geometrical fidelity	944:967	The geometrical fidelity of the fabricated scaffolds	944:995	The geometrical fidelity of the fabricated scaffolds was defined by printability and collapse tests.					
34948280	7	29	theme	multiple	1065:1072	arg1	cross-linkers					1074:1086	multiple cross-linkers	1065:1086	multiple cross-linkers	1065:1086	The effect of using multiple cross-linkers simultaneously was assessed.					
34948280	1	30	theme	scaffolds	102:110	arg1	fidelity					75:82	shape fidelity	69:82	shape fidelity of 3D bio-printed scaffolds with soft biomaterials	69:133	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	0	31	theme	Hybrid	23:28	arg1	Hydrogels					46:54	Hybrid Pre-Crosslinked Hydrogels	23:54	Hybrid Pre-Crosslinked Hydrogels	23:54	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels.					
34948280	0	32	link	Pre-Crosslinked	30:44	arg1	Hydrogels					46:54	Hybrid Pre-Crosslinked Hydrogels	23:54	Hybrid Pre-Crosslinked Hydrogels	23:54	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels.					
34948280	6	33	theme	scaffolds	987:995	arg1	fidelity					960:967	The geometrical fidelity	944:967	The geometrical fidelity of the fabricated scaffolds	944:995	The geometrical fidelity of the fabricated scaffolds was defined by printability and collapse tests.					
34948280	5	34	theme	compositions	930:941	arg1	capacity					874:881	the shear-thinning capacity	855:881	the shear-thinning capacity	855:881	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	34	theme	compositions	930:941	arg1	points					893:898	gelation points	884:898	gelation points	884:898	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	34	theme	compositions	930:941	arg1	rate					914:917	recovery rate	905:917	recovery rate of various compositions	905:941	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	3	35	theme	scaffolds	403:411	arg1	during-					355:361	during-	355:361	during-	355:361	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	35	theme	scaffolds	403:411	arg1	fidelity					387:394	post-printing shape fidelity	367:394	post-printing shape fidelity	367:394	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	1	36	theme	soft	117:120	arg1	biomaterials					122:133	soft biomaterials	117:133	soft biomaterials	117:133	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	4	37	theme	solid	639:643	arg1	content					645:651	The overall solid content	627:651	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC)	627:693	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC) was limited to 6%.					
34948280	0	38	theme	Hydrogels	46:54	arg1	Bio-Printability					3:18	3D Bio-Printability	0:18	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels	0:54	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels.					
34948280	10	39	theme	3D	1497:1498	arg1	bio-fabrication					1500:1514	3D bio-fabrication	1497:1514	3D bio-fabrication of scaffolds	1497:1527	This pre-crosslinking-based rheological property controlling technique can open a new avenue for 3D bio-fabrication of scaffolds, ensuring proper geometry.					
34948280	4	40	theme	Alginate-Carboxymethyl	656:677	arg1	CMC					690:692	CMC	690:692	CMC	690:692	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC) was limited to 6%.					
34948280	4	40	theme	Alginate-Carboxymethyl	656:677	arg1	Cellulose					679:687	Alginate-Carboxymethyl Cellulose	656:687	Alginate-Carboxymethyl Cellulose (CMC)	656:693	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC) was limited to 6%.					
34948280	3	41	theme	viscosity	468:476	arg1	enhancer					478:485	viscosity enhancer	468:485	viscosity enhancer	468:485	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	41	theme	viscosity	468:476	arg1	Cellulose					451:459	Carboxymethyl Cellulose	437:459	Carboxymethyl Cellulose (CMC) (viscosity enhancer)	437:486	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	0	42	theme	Pre-Crosslinked	30:44	arg1	Hydrogels					46:54	Hybrid Pre-Crosslinked Hydrogels	23:54	Hybrid Pre-Crosslinked Hydrogels	23:54	3D Bio-Printability of Hybrid Pre-Crosslinked Hydrogels.					
34948280	3	43	theme	Cellulose	451:459	arg1	salts					510:514	different calcium salts	492:514	different calcium salts (CaCl2 and CaSO4, physical cross-linkers)	492:556	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	43	theme	Cellulose	451:459	arg1	CaSO4					527:531	CaSO4	527:531	CaSO4	527:531	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	43	theme	Cellulose	451:459	arg1	CaCl2					517:521	CaCl2	517:521	CaCl2	517:521	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	43	theme	Cellulose	451:459	arg1	percentages					422:432	various percentages	414:432	various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer)	414:486	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	2	44	theme	rheological	168:178	arg1	investigation					180:192	a rheological investigation	166:192	a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds	166:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	3	45	theme	shape	611:615	arg1	fidelity					617:624	shape fidelity	611:624	shape fidelity	611:624	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	5	46	theme	interval	788:795	arg1	tests					809:813	three interval thixotropic tests	782:813	three interval thixotropic tests	782:813	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	47	theme	tests	771:775	arg1	tests					735:739	rheological tests	723:739	rheological tests	723:739	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	47	theme	tests	771:775	arg1	tests					771:775	amplitude tests	761:775	amplitude tests	761:775	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	47	theme	tests	771:775	arg1	tests					809:813	three interval thixotropic tests	782:813	three interval thixotropic tests	782:813	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	47	theme	tests	771:775	arg1	curves					753:758	flow curves	748:758	flow curves	748:758	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	47	theme	tests	771:775	arg1	set					716:718	A set	714:718	A set	714:718	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	48	theme	thixotropic	797:807	arg1	tests					809:813	three interval thixotropic tests	782:813	three interval thixotropic tests	782:813	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	2	49	theme	mechanical	238:247	arg1	properties					249:258	useful physical and mechanical properties	218:258	useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds	218:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	5	50	theme	gelation	884:891	arg1	points					893:898	gelation points	884:898	gelation points	884:898	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	1	51	with	fidelity	75:82	arg1	biomaterials					122:133	soft biomaterials	117:133	soft biomaterials	117:133	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	2	52	theme	physical	225:232	arg1	properties					249:258	useful physical and mechanical properties	218:258	useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds	218:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	10	53	theme	new	1482:1484	arg1	avenue					1486:1491	a new avenue	1480:1491	a new avenue for 3D bio-fabrication of scaffolds	1480:1527	This pre-crosslinking-based rheological property controlling technique can open a new avenue for 3D bio-fabrication of scaffolds, ensuring proper geometry.					
34948280	8	54	link	pre-crosslinked	1186:1200	arg1	hybrid					1202:1207	a pre-crosslinked hybrid	1184:1207	a pre-crosslinked hybrid	1184:1207	Various large-scale scaffolds were fabricated (up to 5.0 cm) using a pre-crosslinked hybrid.					
34948280	8	55	theme	Various	1117:1123	arg1	scaffolds					1137:1145	Various large-scale scaffolds	1117:1145	Various large-scale scaffolds	1117:1145	Various large-scale scaffolds were fabricated (up to 5.0 cm) using a pre-crosslinked hybrid.					
34948280	2	56	theme	useful	218:223	arg1	properties					249:258	useful physical and mechanical properties	218:258	useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds	218:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	2	57	theme	geometric	284:292	arg1	fidelity					294:301	the geometric fidelity	280:301	the geometric fidelity of 3D bio-printed scaffolds	280:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	3	58	theme	different	492:500	arg1	salts					510:514	different calcium salts	492:514	different calcium salts (CaCl2 and CaSO4, physical cross-linkers)	492:556	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	58	theme	different	492:500	arg1	CaSO4					527:531	CaSO4	527:531	CaSO4	527:531	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	3	58	theme	different	492:500	arg1	CaCl2					517:521	CaCl2	517:521	CaCl2	517:521	To ensure during- and post-printing shape fidelity of the scaffolds, various percentages of Carboxymethyl Cellulose (CMC) (viscosity enhancer) and different calcium salts (CaCl2 and CaSO4, physical cross-linkers) were mixed into alginate before extrusion to realize shape fidelity.					
34948280	5	59	theme	tests	735:739	arg1	tests					735:739	rheological tests	723:739	rheological tests	723:739	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	59	theme	tests	735:739	arg1	tests					771:775	amplitude tests	761:775	amplitude tests	761:775	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	59	theme	tests	735:739	arg1	tests					809:813	three interval thixotropic tests	782:813	three interval thixotropic tests	782:813	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	59	theme	tests	735:739	arg1	curves					753:758	flow curves	748:758	flow curves	748:758	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	59	theme	tests	735:739	arg1	set					716:718	A set	714:718	A set	714:718	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	10	60	theme	scaffolds	1519:1527	arg1	bio-fabrication					1500:1514	3D bio-fabrication	1497:1514	3D bio-fabrication of scaffolds	1497:1527	This pre-crosslinking-based rheological property controlling technique can open a new avenue for 3D bio-fabrication of scaffolds, ensuring proper geometry.					
34948280	2	61	theme	bio-printed	309:319	arg1	scaffolds					321:329	3D bio-printed scaffolds	306:329	3D bio-printed scaffolds	306:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	1	62	theme	ongoing	141:147	arg1	challenge					149:157	an ongoing challenge	138:157	an ongoing challenge	138:157	Maintaining shape fidelity of 3D bio-printed scaffolds with soft biomaterials is an ongoing challenge.					
34948280	8	63	theme	pre-crosslinked	1186:1200	arg1	hybrid					1202:1207	a pre-crosslinked hybrid	1184:1207	a pre-crosslinked hybrid	1184:1207	Various large-scale scaffolds were fabricated (up to 5.0 cm) using a pre-crosslinked hybrid.					
34948280	5	64	theme	tests	809:813	arg1	tests					735:739	rheological tests	723:739	rheological tests	723:739	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	64	theme	tests	809:813	arg1	tests					771:775	amplitude tests	761:775	amplitude tests	761:775	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	64	theme	tests	809:813	arg1	tests					809:813	three interval thixotropic tests	782:813	three interval thixotropic tests	782:813	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	64	theme	tests	809:813	arg1	curves					753:758	flow curves	748:758	flow curves	748:758	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	64	theme	tests	809:813	arg1	set					716:718	A set	714:718	A set	714:718	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	65	theme	various	922:928	arg1	compositions					930:941	various compositions	922:941	various compositions	922:941	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	4	66	theme	overall	631:637	arg1	content					645:651	The overall solid content	627:651	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC)	627:693	The overall solid content of Alginate-Carboxymethyl Cellulose (CMC) was limited to 6%.					
34948280	8	67	theme	large-scale	1125:1135	arg1	scaffolds					1137:1145	Various large-scale scaffolds	1117:1145	Various large-scale scaffolds	1117:1145	Various large-scale scaffolds were fabricated (up to 5.0 cm) using a pre-crosslinked hybrid.					
34948280	2	68	theme	related	269:275	arg1	properties					249:258	useful physical and mechanical properties	218:258	useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds	218:329	Here, a rheological investigation focusing on identifying useful physical and mechanical properties directly related to the geometric fidelity of 3D bio-printed scaffolds is presented.					
34948280	5	69	theme	shear-thinning	859:872	arg1	capacity					874:881	the shear-thinning capacity	855:881	the shear-thinning capacity	855:881	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
34948280	5	70	theme	rheological	723:733	arg1	tests					735:739	rheological tests	723:739	rheological tests	723:739	A set of rheological tests, e.g., flow curves, amplitude tests, and three interval thixotropic tests, were performed to identify and compare the shear-thinning capacity, gelation points, and recovery rate of various compositions.					
33333481	5	0	theme	hydrogel	827:834	arg1	nanocomposite					836:848	the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	793:848	the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	793:848	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed.					
33333481	4	1	dep	infrared	606:613	arg1	FTIR					616:619	FTIR	616:619	FTIR	616:619	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	8	2	theme	S.agalactiae	1019:1030	arg1	damage					1009:1014	The bacterial cell wall damage	985:1014	The bacterial cell wall damage of S.agalactiae 1661	985:1035	The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy.					
33333481	1	3	theme	natural	177:183	arg1	carrageenan					193:203	natural polymer carrageenan	177:203	natural polymer carrageenan	177:203	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed.					
33333481	4	4	theme	Electron	645:652	arg1	Microscopy					654:663	Scanning Electron Microscopy	636:663	Scanning Electron Microscopy (SEM)	636:669	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	4	theme	Electron	645:652	arg1	SEM					666:668	SEM	666:668	SEM	666:668	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	5	5	theme	nanocomposite	836:848	arg1	behaviour					780:788	The swelling behaviour	767:788	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	767:848	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed.					
33333481	1	6	theme	polymer	185:191	arg1	carrageenan					193:203	natural polymer carrageenan	177:203	natural polymer carrageenan	177:203	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed.					
33333481	9	7	theme	A549	1129:1132	arg1	cells					1146:1150	the A549 lung cancer cells	1125:1150	the A549 lung cancer cells	1125:1150	The cytotoxic assay was performed against the A549 lung cancer cells.					
33333481	4	8	theme	Scanning	636:643	arg1	Microscopy					654:663	Scanning Electron Microscopy	636:663	Scanning Electron Microscopy (SEM)	636:669	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	8	theme	Scanning	636:643	arg1	SEM					666:668	SEM	666:668	SEM	666:668	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	5	9	theme	swelling	771:778	arg1	behaviour					780:788	The swelling behaviour	767:788	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	767:848	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed.					
33333481	4	10	dep	Fourier	588:594	arg1	Transform					596:604	Transform	596:604	Transform infrared (FTIR) spectroscopy	596:633	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	8	11	theme	scanning	1053:1060	arg1	microscopy					1071:1080	scanning electron microscopy	1053:1080	scanning electron microscopy	1053:1080	The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy.					
33333481	2	12	theme	capping	327:333	arg1	agent					335:339	capping agent	327:339	capping agent	327:339	The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent.					
33333481	9	13	theme	lung	1134:1137	arg1	cells					1146:1150	the A549 lung cancer cells	1125:1150	the A549 lung cancer cells	1125:1150	The cytotoxic assay was performed against the A549 lung cancer cells.					
33333481	6	14	theme	antibacterial	873:885	arg1	activity					887:894	The antibacterial activity	869:894	The antibacterial activity	869:894	The antibacterial activity was tested against human pathogens viz.					
33333481	9	15	theme	cancer	1139:1144	arg1	cells					1146:1150	the A549 lung cancer cells	1125:1150	the A549 lung cancer cells	1125:1150	The cytotoxic assay was performed against the A549 lung cancer cells.					
33333481	4	16	theme	composite	522:530	arg1	performances					532:543	The manufactured composite performances	505:543	The manufactured composite performances	505:543	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	17	theme	Transmission	719:730	arg1	TEM					753:755	TEM	753:755	TEM	753:755	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	17	theme	Transmission	719:730	arg1	Microscopy					741:750	Transmission Electron Microscopy	719:750	Transmission Electron Microscopy (TEM)	719:756	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	3	18	theme	biohybrid	471:479	arg1	nanocomposite					490:502	the biohybrid hydrogel nanocomposite	467:502	the biohybrid hydrogel nanocomposite	467:502	Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite.					
33333481	3	19	theme	gelatin	385:391	arg1	hydrogel					393:400	gelatin hydrogel	385:400	gelatin hydrogel using glutaraldehyde having a cross-link role	385:446	Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite.					
33333481	3	20	contain	having	423:428	arg1	glutaraldehyde					408:421	glutaraldehyde	408:421	glutaraldehyde having a cross-link role	408:446	Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite.					
33333481	3	20	contain	having	423:428	arg2	role					443:446	a cross-link role	430:446	a cross-link role	430:446	Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite.					
33333481	3	21	theme	hydrogel	481:488	arg1	nanocomposite					490:502	the biohybrid hydrogel nanocomposite	467:502	the biohybrid hydrogel nanocomposite	467:502	Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite.					
33333481	0	22	theme	novel	2:6	arg1	nanocomposite					47:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	8	23	theme	electron	1062:1069	arg1	microscopy					1071:1080	scanning electron microscopy	1053:1080	scanning electron microscopy	1053:1080	The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy.					
33333481	1	24	theme	gelatin	209:215	arg1	protein					217:223	gelatin protein	209:223	gelatin protein	209:223	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed.					
33333481	3	25	theme	cross-link	432:441	arg1	role					443:446	a cross-link role	430:446	a cross-link role	430:446	Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite.					
33333481	0	26	theme	hybrid	31:36	arg1	nanocomposite					47:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	4	27	theme	X-ray	690:694	arg1	spectroscopy					702:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	0	28	theme	Ag/carrageenan-gelatin	8:29	arg1	nanocomposite					47:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	6	29	dep	pathogens	921:929	arg1	viz					931:933	viz	931:933	human pathogens viz	915:933	The antibacterial activity was tested against human pathogens viz.					
33333481	4	30	theme	dispersive	679:688	arg1	spectroscopy					702:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	31	theme	EDX	697:699	arg1	spectroscopy					702:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	0	32	theme	hydrogel	38:45	arg1	nanocomposite					47:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite	0:59	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	4	33	theme	manufactured	509:520	arg1	performances					532:543	The manufactured composite performances	505:543	The manufactured composite performances	505:543	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	8	34	theme	bacterial	989:997	arg1	damage					1009:1014	The bacterial cell wall damage	985:1014	The bacterial cell wall damage of S.agalactiae 1661	985:1035	The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy.					
33333481	4	35	dep	spectroscopy	574:585	arg1	methods					758:764	methods	758:764	methods	758:764	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	5	36	theme	hybrid	820:825	arg1	nanocomposite					836:848	the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	793:848	the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	793:848	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed.					
33333481	0	37	dep	nanocomposite	47:59	arg1	characterization					110:125	characterization	110:125	characterization	110:125	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	0	37	dep	nanocomposite	47:59	arg1	Preparation					94:104	Preparation	94:104	Preparation	94:104	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	1	38	theme	novel	130:134	arg1	nanocomposite					155:167	A novel biohybrid hydrogel nanocomposite	128:167	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein	128:223	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed.					
33333481	2	39	theme	silver	245:250	arg1	nanoparticles					252:264	The silver nanoparticles	241:264	The silver nanoparticles	241:264	The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent.					
33333481	8	40	theme	wall	1004:1007	arg1	damage					1009:1014	The bacterial cell wall damage	985:1014	The bacterial cell wall damage of S.agalactiae 1661	985:1035	The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy.					
33333481	1	41	theme	biohybrid	136:144	arg1	nanocomposite					155:167	A novel biohybrid hydrogel nanocomposite	128:167	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein	128:223	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed.					
33333481	9	42	theme	cytotoxic	1087:1095	arg1	assay					1097:1101	The cytotoxic assay	1083:1101	The cytotoxic assay	1083:1101	The cytotoxic assay was performed against the A549 lung cancer cells.					
33333481	4	43	theme	infrared	606:613	arg1	spectroscopy					622:633	infrared (FTIR) spectroscopy	606:633	infrared (FTIR) spectroscopy	606:633	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	6	44	theme	human	915:919	arg1	pathogens					921:929	human pathogens	915:929	human pathogens viz	915:933	The antibacterial activity was tested against human pathogens viz.					
33333481	8	45	theme	cell	999:1002	arg1	damage					1009:1014	The bacterial cell wall damage	985:1014	The bacterial cell wall damage of S.agalactiae 1661	985:1035	The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy.					
33333481	1	46	theme	hydrogel	146:153	arg1	nanocomposite					155:167	A novel biohybrid hydrogel nanocomposite	128:167	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein	128:223	A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed.					
33333481	2	47	theme	carrageenan	290:300	arg1	polymer					302:308	the carrageenan polymer	286:308	the carrageenan polymer	286:308	The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent.					
33333481	5	48	theme	Ag/Carrageenan-gelatin	797:818	arg1	nanocomposite					836:848	the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	793:848	the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite	793:848	The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed.					
33333481	4	49	theme	Energy	672:677	arg1	spectroscopy					702:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	Energy dispersive X-ray (EDX) spectroscopy	672:713	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	0	50	theme	biological	69:78	arg1	applications					80:91	its biological applications	65:91	its biological applications	65:91	A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization.					
33333481	4	51	theme	UV-visible	563:572	arg1	spectroscopy					574:585	UV-visible spectroscopy	563:585	UV-visible spectroscopy	563:585	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	52	theme	Electron	732:739	arg1	TEM					753:755	TEM	753:755	TEM	753:755	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
33333481	4	52	theme	Electron	732:739	arg1	Microscopy					741:750	Transmission Electron Microscopy	719:750	Transmission Electron Microscopy (TEM)	719:756	The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods.					
34364563	6	0	theme	potential	975:983	arg1	production					985:994	the potential production	971:994	the potential production of superhydrophilic materials	971:1024	The method is easy to carry out and can be used for the potential production of superhydrophilic materials.					
34364563	4	1	theme	SiO2	598:601	arg1	nanoparticles					603:615	SiO2 nanoparticles	598:615	SiO2 nanoparticles that were dissolved in 75% ethanol	598:650	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	1	2	theme	transparent	174:184	arg1	materials					218:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	0	3	theme	ethanol	101:107	arg1	process					117:123	a simple ethanol soaking process	92:123	a simple ethanol soaking process	92:123	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	4	4	theme	contact	663:669	arg1	WCA					678:680	WCA	678:680	WCA	678:680	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	4	4	theme	contact	663:669	arg1	angle					671:675	its water contact angle	653:675	its water contact angle (WCA)	653:681	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	0	5	theme	simple	94:99	arg1	process					117:123	a simple ethanol soaking process	92:123	a simple ethanol soaking process	92:123	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	3	6	theme	SiO2	500:503	arg1	nanoparticles					505:517	SiO2 nanoparticles	500:517	SiO2 nanoparticles	500:517	The composite film was obtained by soaking a chitosan-sodium phytate film in an ethanol solution of SiO2 nanoparticles.					
34364563	5	7	theme	oxygen	804:809	arg1	OTR					830:832	OTR	830:832	OTR	830:832	Furthermore, the oxygen transmission rate (OTR) was significantly reduced, and the mechanical properties of the film were improved.					
34364563	5	7	theme	oxygen	804:809	arg1	rate					824:827	the oxygen transmission rate	800:827	the oxygen transmission rate (OTR)	800:833	Furthermore, the oxygen transmission rate (OTR) was significantly reduced, and the mechanical properties of the film were improved.					
34364563	4	8	theme	%	642:642	arg1	ethanol					644:650	75% ethanol	640:650	75% ethanol	640:650	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	6	9	theme	superhydrophilic	999:1014	arg1	materials					1016:1024	superhydrophilic materials	999:1024	superhydrophilic materials	999:1024	The method is easy to carry out and can be used for the potential production of superhydrophilic materials.					
34364563	1	10	theme	superhydrophilic	186:201	arg1	materials					218:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	0	11	theme	soaking	109:115	arg1	process					117:123	a simple ethanol soaking process	92:123	a simple ethanol soaking process	92:123	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	1	12	theme	food	203:206	arg1	materials					218:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	4	13	theme	water	657:661	arg1	WCA					678:680	WCA	678:680	WCA	678:680	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	4	13	theme	water	657:661	arg1	angle					671:675	its water contact angle	653:675	its water contact angle (WCA)	653:681	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	4	14	dep	3°	708:709	arg1	to					705:706	to	705:706	to	705:706	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	1	15	theme	packaging	208:216	arg1	materials					218:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	3	16	theme	composite	404:412	arg1	film					414:417	The composite film	400:417	The composite film	400:417	The composite film was obtained by soaking a chitosan-sodium phytate film in an ethanol solution of SiO2 nanoparticles.					
34364563	6	17	used	used	962:965	arg2	easy					933:936	easy	933:936	easy	933:936	The method is easy to carry out and can be used for the potential production of superhydrophilic materials.					
34364563	6	17	used	used	962:965	arg2	method					923:928	The method	919:928	The method	919:928	The method is easy to carry out and can be used for the potential production of superhydrophilic materials.					
34364563	5	18	theme	film	899:902	arg1	properties					881:890	the mechanical properties	866:890	the mechanical properties of the film	866:902	Furthermore, the oxygen transmission rate (OTR) was significantly reduced, and the mechanical properties of the film were improved.					
34364563	1	19	theme	materials	218:226	arg1	development					130:140	The development	126:140	The development of environmentally friendly and transparent superhydrophilic food packaging materials	126:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	1	19	theme	materials	218:226	arg1	essential					231:239	essential	231:239	essential	231:239	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	0	20	theme	superhydrophilic	17:32	arg1	SiO2					34:37	a superhydrophilic SiO2	15:37	a superhydrophilic SiO2	15:37	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	4	21	theme	phytate	569:575	arg1	film					577:580	the chitosan-sodium phytate film	549:580	the chitosan-sodium phytate film	549:580	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	4	22	theme	film	720:723	arg1	surface					725:731	the film surface	716:731	the film surface	716:731	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	6	23	theme	materials	1016:1024	arg1	production					985:994	the potential production	971:994	the potential production of superhydrophilic materials	971:1024	The method is easy to carry out and can be used for the potential production of superhydrophilic materials.					
34364563	2	24	theme	simple	306:311	arg1	method					313:318	a simple method	304:318	a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film	304:397	The objective of this study was to develop a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film.					
34364563	3	25	theme	ethanol	480:486	arg1	solution					488:495	an ethanol solution	477:495	an ethanol solution of SiO2 nanoparticles	477:517	The composite film was obtained by soaking a chitosan-sodium phytate film in an ethanol solution of SiO2 nanoparticles.					
34364563	5	26	theme	mechanical	870:879	arg1	properties					881:890	the mechanical properties	866:890	the mechanical properties of the film	866:902	Furthermore, the oxygen transmission rate (OTR) was significantly reduced, and the mechanical properties of the film were improved.					
34364563	5	27	theme	transmission	811:822	arg1	OTR					830:832	OTR	830:832	OTR	830:832	Furthermore, the oxygen transmission rate (OTR) was significantly reduced, and the mechanical properties of the film were improved.					
34364563	5	27	theme	transmission	811:822	arg1	rate					824:827	the oxygen transmission rate	800:827	the oxygen transmission rate (OTR)	800:833	Furthermore, the oxygen transmission rate (OTR) was significantly reduced, and the mechanical properties of the film were improved.					
34364563	0	28	theme	SiO2	34:37	arg1	Preparation					0:10	Preparation	0:10	Preparation of a superhydrophilic SiO2	0:37	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	0	29	theme	chitosan-sodium	60:74	arg1	film					84:87	coated chitosan-sodium phytate film	53:87	coated chitosan-sodium phytate film	53:87	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	3	30	theme	chitosan-sodium	445:459	arg1	film					469:472	a chitosan-sodium phytate film	443:472	a chitosan-sodium phytate film	443:472	The composite film was obtained by soaking a chitosan-sodium phytate film in an ethanol solution of SiO2 nanoparticles.					
34364563	2	31	theme	transparent	353:363	arg1	film					394:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	The objective of this study was to develop a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film.					
34364563	0	32	theme	coated	53:58	arg1	film					84:87	coated chitosan-sodium phytate film	53:87	coated chitosan-sodium phytate film	53:87	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	4	33	theme	chitosan-sodium	553:567	arg1	film					577:580	the chitosan-sodium phytate film	549:580	the chitosan-sodium phytate film	549:580	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	3	34	theme	nanoparticles	505:517	arg1	solution					488:495	an ethanol solution	477:495	an ethanol solution of SiO2 nanoparticles	477:517	The composite film was obtained by soaking a chitosan-sodium phytate film in an ethanol solution of SiO2 nanoparticles.					
34364563	2	35	theme	study	283:287	arg1	objective					265:273	The objective	261:273	The objective of this study	261:287	The objective of this study was to develop a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film.					
34364563	1	36	from	essential	231:239	arg1	lives					254:258	our daily lives	244:258	our daily lives	244:258	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	2	37	theme	superhydrophilic	335:350	arg1	film					394:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	The objective of this study was to develop a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film.					
34364563	1	38	theme	daily	248:252	arg1	lives					254:258	our daily lives	244:258	our daily lives	244:258	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	1	39	from	lives	254:258	arg1	development					130:140	The development	126:140	The development of environmentally friendly and transparent superhydrophilic food packaging materials	126:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	1	39	from	lives	254:258	arg1	essential					231:239	essential	231:239	essential	231:239	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	1	40	theme	friendly	161:168	arg1	materials					218:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	environmentally friendly and transparent superhydrophilic food packaging materials	145:226	The development of environmentally friendly and transparent superhydrophilic food packaging materials is essential in our daily lives.					
34364563	2	41	theme	composite	384:392	arg1	film					394:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	The objective of this study was to develop a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film.					
34364563	3	42	theme	phytate	461:467	arg1	film					469:472	a chitosan-sodium phytate film	443:472	a chitosan-sodium phytate film	443:472	The composite film was obtained by soaking a chitosan-sodium phytate film in an ethanol solution of SiO2 nanoparticles.					
34364563	4	43	theme	75	640:641	arg1	%					642:642	%	642:642	%	642:642	The results showed that when the chitosan-sodium phytate film was coated with SiO2 nanoparticles that were dissolved in 75% ethanol, its water contact angle (WCA) was reduced from 100° to 3°, and the film surface was changed from a hydrophobic to a superhydrophilic.					
34364563	0	44	theme	phytate	76:82	arg1	film					84:87	coated chitosan-sodium phytate film	53:87	coated chitosan-sodium phytate film	53:87	Preparation of a superhydrophilic SiO2 nanoparticles coated chitosan-sodium phytate film by a simple ethanol soaking process.					
34364563	2	45	theme	biodegradable	370:382	arg1	film					394:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	a superhydrophilic, transparent, and biodegradable composite film	333:397	The objective of this study was to develop a simple method of preparing a superhydrophilic, transparent, and biodegradable composite film.					
32041216	0	0	theme	Phospholipid	77:88	arg1	Hydrogel					122:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel	58:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery	58:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	3	1	theme	hydrogel	515:522	arg1	type					460:463	a type	458:463	a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH)	458:531	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	6	2	theme	TSPMAH	1077:1082	arg1	Characterizations					1052:1068	Characterizations	1052:1068	Characterizations of the TSPMAH	1052:1082	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
32041216	0	3	theme	Thermo-Responsible	58:75	arg1	Hydrogel					122:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel	58:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery	58:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	2	4	from	liquids	329:335	arg1	unstable					295:302	unstable	295:302	unstable	295:302	However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery.					
32041216	9	5	theme	carrier	1509:1515	arg1	system					1517:1522	a drug carrier system	1502:1522	a drug carrier system	1502:1522	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	0	6	theme	Composite	112:120	arg1	Hydrogel					122:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel	58:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery	58:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	3	7	theme	thermo-responsive	545:561	arg1	liposomes					563:571	thermo-responsive liposomes	545:571	thermo-responsive liposomes onto alginates	545:586	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	0	8	theme	Microcapsule-Alginate	90:110	arg1	Hydrogel					122:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel	58:129	Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery	58:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	5	9	theme	PS-Ca2+-Carboxyl-alginate	1013:1037	arg1	interaction					1039:1049	PS-Ca2+-Carboxyl-alginate interaction	1013:1049	PS-Ca2+-Carboxyl-alginate interaction	1013:1049	The TSPMAH was then successfully prepared by bridge-linking the microcapsules onto the alginate hydrogel via PS-Ca2+-Carboxyl-alginate interaction.					
32041216	10	10	theme	delivery	1731:1738	arg1	system					1740:1745	an intelligent drug delivery system	1711:1745	an intelligent drug delivery system	1711:1745	Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.					
32041216	1	11	used	used	176:179	arg2	Liposomes					150:158	Liposomes	150:158	Liposomes	150:158	Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering.					
32041216	4	12	theme	liposomes	734:742	arg1	temperature-sensitivity					703:725	The temperature-sensitivity	699:725	The temperature-sensitivity of the liposomes	699:742	The temperature-sensitivity of the liposomes was actualized by using phospholipids comprising dipalmitoylphosphatidylcholine (DPPC) and PS and the liposomes were prepared by a thin-film dispersion method.					
32041216	3	13	theme	liposome-alginate	487:503	arg1	TSPMAH					525:530	TSPMAH	525:530	TSPMAH	525:530	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	13	theme	liposome-alginate	487:503	arg1	hydrogel					515:522	thermo-responsible liposome-alginate composite hydrogel	468:522	thermo-responsible liposome-alginate composite hydrogel (TSPMAH)	468:531	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	6	14	theme	laser	1174:1178	arg1	microscopy					1198:1207	laser scanning confocal microscopy	1174:1207	laser scanning confocal microscopy	1174:1207	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
32041216	10	15	contain	has	1661:1663	arg2	potentials					1671:1680	great potentials	1665:1680	great potentials	1665:1680	Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.					
32041216	10	15	contain	has	1661:1663	arg1	TSPMAH					1637:1642	the TSPMAH	1633:1642	the TSPMAH described herein	1633:1659	Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.					
32041216	8	16	theme	composite	1351:1359	arg1	biocompatible					1374:1386	biocompatible	1374:1386	biocompatible	1374:1386	Cytotoxicity evaluations of the TSPMAH showed that the composite hydrogel was biocompatible, safe, and non-toxic.					
32041216	8	16	theme	composite	1351:1359	arg1	hydrogel					1361:1368	the composite hydrogel	1347:1368	the composite hydrogel	1347:1368	Cytotoxicity evaluations of the TSPMAH showed that the composite hydrogel was biocompatible, safe, and non-toxic.					
32041216	1	17	theme	drug	184:187	arg1	delivery					189:196	drug delivery	184:196	drug delivery	184:196	Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering.					
32041216	0	18	theme	Hydrogel	122:129	arg1	Type					50:53	a Novel Type	42:53	a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery	42:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	9	19	theme	drug	1504:1507	arg1	system					1517:1522	a drug carrier system	1502:1522	a drug carrier system	1502:1522	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	3	20	from	serine	644:649	arg1	membrane					672:679	the liposome membrane	659:679	the liposome membrane	659:679	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	2	21	theme	loading	389:395	arg1	capacities					397:406	low loading capacities	385:406	low loading capacities when used for drug delivery	385:434	However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery.					
32041216	10	22	theme	intelligent	1714:1724	arg1	system					1740:1745	an intelligent drug delivery system	1711:1745	an intelligent drug delivery system	1711:1745	Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.					
32041216	4	23	theme	dispersion	885:894	arg1	method					896:901	a thin-film dispersion method	873:901	a thin-film dispersion method	873:901	The temperature-sensitivity of the liposomes was actualized by using phospholipids comprising dipalmitoylphosphatidylcholine (DPPC) and PS and the liposomes were prepared by a thin-film dispersion method.					
32041216	2	24	theme	low	385:387	arg1	capacities					397:406	low loading capacities	385:406	low loading capacities when used for drug delivery	385:434	However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery.					
32041216	10	25	theme	drug	1726:1729	arg1	system					1740:1745	an intelligent drug delivery system	1711:1745	an intelligent drug delivery system	1711:1745	Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.					
32041216	4	26	theme	thin-film	875:883	arg1	method					896:901	a thin-film dispersion method	873:901	a thin-film dispersion method	873:901	The temperature-sensitivity of the liposomes was actualized by using phospholipids comprising dipalmitoylphosphatidylcholine (DPPC) and PS and the liposomes were prepared by a thin-film dispersion method.					
32041216	6	27	dep	transform	1137:1145	arg1	infrared					1147:1154	infrared	1147:1154	transform infrared spectroscopy	1137:1167	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
32041216	8	28	theme	Cytotoxicity	1296:1307	arg1	evaluations					1309:1319	Cytotoxicity evaluations	1296:1319	Cytotoxicity evaluations of the TSPMAH	1296:1333	Cytotoxicity evaluations of the TSPMAH showed that the composite hydrogel was biocompatible, safe, and non-toxic.					
32041216	9	29	theme	thermos-inducible	1431:1447	arg1	release					1449:1455	thermos-inducible release	1431:1455	thermos-inducible release	1431:1455	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	5	30	theme	alginate	991:998	arg1	hydrogel					1000:1007	the alginate hydrogel	987:1007	the alginate hydrogel	987:1007	The TSPMAH was then successfully prepared by bridge-linking the microcapsules onto the alginate hydrogel via PS-Ca2+-Carboxyl-alginate interaction.					
32041216	2	31	theme	drug	422:425	arg1	delivery					427:434	drug delivery	422:434	drug delivery	422:434	However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery.					
32041216	0	32	theme	Drug	135:138	arg1	Delivery					140:147	Drug Delivery	135:147	Drug Delivery	135:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	9	33	theme	complex-carrying	1567:1582	arg1	carriers					1596:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	3	34	from	alginate	689:696	arg1	membrane					672:679	the liposome membrane	659:679	the liposome membrane	659:679	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	9	35	theme	TSPMAH	1584:1589	arg1	carriers					1596:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	1	36	used	used	226:229	arg2	alginates					205:213	alginates	205:213	alginates	205:213	Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering.					
32041216	6	37	theme	electron	1116:1123	arg1	microscopy					1125:1134	scanning electron microscopy	1107:1134	scanning electron microscopy	1107:1134	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
32041216	9	38	theme	model	1460:1464	arg1	drugs					1466:1470	model drugs	1460:1470	model drugs encapsulated by the TSPMAH as a drug carrier system	1460:1522	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	6	39	theme	scanning	1107:1114	arg1	microscopy					1125:1134	scanning electron microscopy	1107:1134	scanning electron microscopy	1107:1134	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
32041216	0	40	theme	Novel	44:48	arg1	Type					50:53	a Novel Type	42:53	a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery	42:147	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	9	41	theme	drugs	1466:1470	arg1	release					1449:1455	thermos-inducible release	1431:1455	thermos-inducible release	1431:1455	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	9	41	theme	drugs	1466:1470	arg1	loading					1419:1425	loading	1419:1425	loading	1419:1425	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	3	42	theme	Ca2+	597:600	arg1	bonding					611:617	Ca2+ mediated bonding	597:617	Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate	597:696	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	2	43	theme	drug	348:351	arg1	carriers					353:360	the drug carriers	344:360	the drug carriers made of alginates	344:378	However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery.					
32041216	7	44	theme	rheological	1230:1240	arg1	property					1242:1249	Their rheological property	1224:1249	Their rheological property	1224:1249	Their rheological property was also characterized by using a rheometer.					
32041216	3	45	theme	mediated	602:609	arg1	bonding					611:617	Ca2+ mediated bonding	597:617	Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate	597:696	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	46	theme	composite	505:513	arg1	TSPMAH					525:530	TSPMAH	525:530	TSPMAH	525:530	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	46	theme	composite	505:513	arg1	hydrogel					515:522	thermo-responsible liposome-alginate composite hydrogel	468:522	thermo-responsible liposome-alginate composite hydrogel (TSPMAH)	468:531	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	47	theme	liposome	663:670	arg1	membrane					672:679	the liposome membrane	659:679	the liposome membrane	659:679	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	48	theme	thermo-responsible	468:485	arg1	TSPMAH					525:530	TSPMAH	525:530	TSPMAH	525:530	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	48	theme	thermo-responsible	468:485	arg1	hydrogel					515:522	thermo-responsible liposome-alginate composite hydrogel	468:522	thermo-responsible liposome-alginate composite hydrogel (TSPMAH)	468:531	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	8	49	theme	TSPMAH	1328:1333	arg1	evaluations					1309:1319	Cytotoxicity evaluations	1296:1319	Cytotoxicity evaluations of the TSPMAH	1296:1333	Cytotoxicity evaluations of the TSPMAH showed that the composite hydrogel was biocompatible, safe, and non-toxic.					
32041216	1	50	theme	tissue	234:239	arg1	engineering					241:251	tissue engineering	234:251	tissue engineering	234:251	Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering.					
32041216	10	51	theme	great	1665:1669	arg1	potentials					1671:1680	great potentials	1665:1680	great potentials	1665:1680	Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.					
32041216	9	52	theme	drug	1591:1594	arg1	carriers					1596:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	9	53	theme	protamine-siRNA	1551:1565	arg1	carriers					1596:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	protamine-siRNA complex-carrying TSPMAH drug carriers	1551:1603	Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine-siRNA complex-carrying TSPMAH drug carriers.					
32041216	6	54	theme	confocal	1189:1196	arg1	microscopy					1198:1207	laser scanning confocal microscopy	1174:1207	laser scanning confocal microscopy	1174:1207	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
32041216	2	55	from	unstable	295:302	arg1	liquids					329:335	liquids	329:335	liquids	329:335	However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery.					
32041216	0	56	theme	Type	50:53	arg1	Synthesis					8:16	Synthesis	8:16	Synthesis	8:16	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	0	56	theme	Type	50:53	arg1	Design					0:5	Design	0:5	Design	0:5	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	0	56	theme	Type	50:53	arg1	Characterization					22:37	Characterization	22:37	Characterization	22:37	Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule-Alginate Composite Hydrogel for Drug Delivery.					
32041216	3	57	theme	phosphatidic	631:642	arg1	PS					652:653	PS	652:653	PS	652:653	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	3	57	theme	phosphatidic	631:642	arg1	serine					644:649	the phosphatidic serine	627:649	the phosphatidic serine (PS) in the liposome membrane	627:679	Herein, we developed a type of thermo-responsible liposome-alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate.					
32041216	6	58	theme	scanning	1180:1187	arg1	microscopy					1198:1207	laser scanning confocal microscopy	1174:1207	laser scanning confocal microscopy	1174:1207	Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively.					
33452968	0	0	theme	Renealmia	76:84	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	0	0	theme	Renealmia	76:84	arg1	composition					61:71	chemical composition	52:71	chemical composition	52:71	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	2	1	theme	extracts	342:349	arg1	composition					323:333	The chemical composition	310:333	The chemical composition of the extracts	310:349	The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer.					
33452968	1	2	theme	extracts	243:250	arg1	activity					186:193	the anti-inflammatory activity	164:193	the anti-inflammatory activity	164:193	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	1	2	theme	extracts	243:250	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	1	3	dep	in	281:282	arg1	vitro					284:288	vitro	284:288	vitro	284:288	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	9	4	theme	IL-6	1581:1584	arg1	concentration					1586:1598	IL-6 concentration	1581:1598	IL-6 concentration	1581:1598	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	5	5	theme	petasites	950:958	arg1	extracts					935:942	the hydroethanolic extracts	916:942	the hydroethanolic extracts of R. petasites	916:958	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	5	6	theme	pouch	827:831	arg1	model					833:837	the air pouch model	819:837	the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites	819:958	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	1	7	theme	Renealmia	255:263	arg1	petasites					265:273	Renealmia petasites	255:273	Renealmia petasites	255:273	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	5	8	theme	hydroethanolic	920:933	arg1	extracts					935:942	the hydroethanolic extracts	916:942	the hydroethanolic extracts of R. petasites	916:958	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	10	9	theme	R.	1651:1652	arg1	petasites					1654:1662	R. petasites	1651:1662	R. petasites	1651:1662	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	1	10	theme	petasites	265:273	arg1	activity					186:193	the anti-inflammatory activity	164:193	the anti-inflammatory activity	164:193	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	1	10	theme	petasites	265:273	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	4	11	from	influence	616:624	arg1	production					633:642	the production	629:642	the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	629:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	5	12	dep	In	781:782	arg1	vivo					784:787	vivo	784:787	vivo	784:787	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	5	13	theme	air	823:825	arg1	model					833:837	the air pouch model	819:837	the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites	819:958	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	6	14	theme	protein	1000:1006	arg1	exudation					1008:1016	protein exudation	1000:1016	protein exudation	1000:1016	After 4 and 24 h, the cellular influx, protein exudation, cytokines, and nitric oxide were evaluated.					
33452968	2	15	theme	iron	381:384	arg1	spectrometer					396:407	a linear iron trap mass spectrometer	372:407	a linear iron trap mass spectrometer	372:407	The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer.					
33452968	7	16	theme	R.	1114:1115	arg1	extracts					1127:1134	the R. petasites extracts	1110:1134	the R. petasites extracts	1110:1134	Eight compounds were tentatively identified in the R. petasites extracts, suggesting five diarylheptanoids, one flavonoid, and two fatty alcohols.					
33452968	1	17	dep	in	294:295	arg1	vivo					297:300	vivo	297:300	vivo	297:300	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	7	18	theme	petasites	1117:1125	arg1	extracts					1127:1134	the R. petasites extracts	1110:1134	the R. petasites extracts	1110:1134	Eight compounds were tentatively identified in the R. petasites extracts, suggesting five diarylheptanoids, one flavonoid, and two fatty alcohols.					
33452968	8	19	theme	intracellular	1319:1331	arg1	actions					1333:1339	their intracellular actions	1313:1339	their intracellular actions	1313:1339	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	1	20	theme	anti-inflammatory	168:184	arg1	activity					186:193	the anti-inflammatory activity	164:193	the anti-inflammatory activity	164:193	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	9	21	theme	air	1607:1609	arg1	model					1617:1621	the air pouch model	1603:1621	the air pouch model	1603:1621	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	4	22	theme	nitric	671:676	arg1	oxide					678:682	nitric oxide	671:682	nitric oxide (NO)	671:687	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	22	theme	nitric	671:676	arg1	NO					685:686	NO	685:686	NO	685:686	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	1	23	dep	composition	148:158	arg1	the					135:137	the	135:137	the	135:137	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	6	24	theme	nitric	1034:1039	arg1	oxide					1041:1045	nitric oxide	1034:1045	nitric oxide	1034:1045	After 4 and 24 h, the cellular influx, protein exudation, cytokines, and nitric oxide were evaluated.					
33452968	0	25	theme	In	0:1	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	4	26	theme	lipopolysaccharide-stimulated	559:587	arg1	macrophages					589:599	lipopolysaccharide-stimulated macrophages	559:599	lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	559:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	9	27	theme	pouch	1611:1615	arg1	model					1617:1621	the air pouch model	1603:1621	the air pouch model	1603:1621	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	5	28	theme	extracts	935:942	arg1	50 mg/kg					904:911	50 mg/kg	904:911	50 mg/kg of the hydroethanolic extracts of R. petasites	904:958	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	9	29	theme	leukocytes	1515:1524	arg1	NO					1566:1567	NO	1566:1567	NO	1566:1567	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	9	29	theme	leukocytes	1515:1524	arg1	exudation					1555:1563	protein exudation	1547:1563	protein exudation	1547:1563	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	9	29	theme	leukocytes	1515:1524	arg1	influx					1505:1510	the influx	1501:1510	the influx of leukocytes, mainly neutrophils	1501:1544	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	9	29	theme	leukocytes	1515:1524	arg1	concentration					1586:1598	IL-6 concentration	1581:1598	IL-6 concentration	1581:1598	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	9	29	theme	leukocytes	1515:1524	arg1	TNF-α					1570:1574	TNF-α	1570:1574	TNF-α	1570:1574	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	3	30	theme	phenolic	416:423	arg1	Total					410:414	Total phenolic	410:423	Total phenolic	410:423	Total phenolic, flavonoid, and tannin content were determined by spectrophotometry analyses.					
33452968	0	31	theme	in	13:14	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	1	32	theme	in	281:282	arg1	assays					302:307	in vitro and in vivo assays	281:307	in vitro and in vivo assays	281:307	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	4	33	dep	In	503:504	arg1	vitro					506:510	vitro	506:510	vitro	506:510	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	34	dep	cytokines	715:723	arg1	TNF-α					748:752	TNF-α	748:752	TNF-α	748:752	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	34	dep	cytokines	715:723	arg1	interleukin-6					759:771	interleukin-6	759:771	interleukin-6 (IL-6)	759:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	34	dep	cytokines	715:723	arg1	factor					740:745	tumor necrosis factor	725:745	tumor necrosis factor (TNF-α)	725:753	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	34	dep	cytokines	715:723	arg1	cytokines					715:723	the pro-inflammatory cytokines	694:723	the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	694:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	34	dep	cytokines	715:723	arg1	IL-6					774:777	IL-6	774:777	IL-6	774:777	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	3	35	theme	tannin	441:446	arg1	content					448:454	tannin content	441:454	tannin content	441:454	Total phenolic, flavonoid, and tannin content were determined by spectrophotometry analyses.					
33452968	8	36	dep	in	1214:1215	arg1	vitro					1217:1221	vitro	1217:1221	vitro	1217:1221	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	5	37	theme	R.	947:948	arg1	petasites					950:958	R. petasites	947:958	R. petasites	947:958	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	1	38	theme	hydroethanolic	202:215	arg1	rhizomes					217:224	the hydroethanolic rhizomes	198:224	the hydroethanolic rhizomes	198:224	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	0	39	theme	anti-inflammatory	21:37	arg1	activity					39:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity	0:46	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	4	40	theme	pro-inflammatory	698:713	arg1	cytokines					715:723	the pro-inflammatory cytokines	694:723	the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	694:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	40	theme	pro-inflammatory	698:713	arg1	factor					740:745	tumor necrosis factor	725:745	tumor necrosis factor (TNF-α)	725:753	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	40	theme	pro-inflammatory	698:713	arg1	interleukin-6					759:771	interleukin-6	759:771	interleukin-6 (IL-6)	759:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	2	41	theme	mass	391:394	arg1	spectrometer					396:407	a linear iron trap mass spectrometer	372:407	a linear iron trap mass spectrometer	372:407	The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer.					
33452968	8	42	theme	important	1373:1381	arg1	mediators					1383:1391	important mediators	1373:1391	important mediators	1373:1391	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	42	theme	important	1373:1381	arg1	NO					1430:1431	NO	1430:1431	NO	1430:1431	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	42	theme	important	1373:1381	arg1	TNF-α					1439:1443	TNF-α	1439:1443	TNF-α	1439:1443	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	42	theme	important	1373:1381	arg1	IL-6					1450:1453	IL-6	1450:1453	IL-6	1450:1453	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	42	theme	important	1373:1381	arg1	O2-					1434:1436	O2-	1434:1436	O2-	1434:1436	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	43	theme	in	1214:1215	arg1	results					1223:1229	The in vitro results	1210:1229	The in vitro results	1210:1229	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	1	44	theme	rhizomes	217:224	arg1	activity					186:193	the anti-inflammatory activity	164:193	the anti-inflammatory activity	164:193	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	1	44	theme	rhizomes	217:224	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	4	45	theme	oxide	678:682	arg1	production					633:642	the production	629:642	the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	629:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	1	46	theme	in	294:295	arg1	assays					302:307	in vitro and in vivo assays	281:307	in vitro and in vivo assays	281:307	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	10	47	theme	diseases	1788:1795	arg1	management					1736:1745	management	1736:1745	management	1736:1745	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	10	47	theme	diseases	1788:1795	arg1	treatment					1722:1730	treatment	1722:1730	treatment	1722:1730	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	6	48	theme	cellular	983:990	arg1	influx					992:997	the cellular influx	979:997	the cellular influx	979:997	After 4 and 24 h, the cellular influx, protein exudation, cytokines, and nitric oxide were evaluated.					
33452968	2	49	theme	trap	386:389	arg1	spectrometer					396:407	a linear iron trap mass spectrometer	372:407	a linear iron trap mass spectrometer	372:407	The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer.					
33452968	5	50	theme	In	781:782	arg1	effects					789:795	In vivo effects	781:795	In vivo effects	781:795	In vivo effects were determined using the air pouch model in which were inoculated carrageenan and thereafter treated with 50 mg/kg of the hydroethanolic extracts of R. petasites.					
33452968	4	51	theme	anti-inflammatory	512:528	arg1	activity					530:537	In vitro anti-inflammatory activity	503:537	In vitro anti-inflammatory activity	503:537	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	10	52	theme	inflammatory	1775:1786	arg1	diseases					1788:1795	other inflammatory diseases	1769:1795	other inflammatory diseases	1769:1795	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	9	53	theme	protein	1547:1553	arg1	exudation					1555:1563	protein exudation	1547:1563	protein exudation	1547:1563	In vivo, R. petasites significantly decrease the influx of leukocytes, mainly neutrophils, protein exudation, NO, TNF-α, and IL-6 concentration in the air pouch model.					
33452968	4	54	theme	superoxide	647:656	arg1	O2-					665:667	O2-	665:667	O2-	665:667	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	54	theme	superoxide	647:656	arg1	anion					658:662	superoxide anion	647:662	superoxide anion (O2-)	647:668	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	55	theme	cytokines	715:723	arg1	production					633:642	the production	629:642	the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	629:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	56	theme	In	503:504	arg1	activity					530:537	In vitro anti-inflammatory activity	503:537	In vitro anti-inflammatory activity	503:537	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	10	57	theme	other	1769:1773	arg1	diseases					1788:1795	other inflammatory diseases	1769:1795	other inflammatory diseases	1769:1795	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	8	58	theme	mediators	1383:1391	arg1	production					1359:1368	the production	1355:1368	the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6	1355:1453	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	3	59	theme	spectrophotometry	475:491	arg1	analyses					493:500	spectrophotometry analyses	475:500	spectrophotometry analyses	475:500	Total phenolic, flavonoid, and tannin content were determined by spectrophotometry analyses.					
33452968	10	60	theme	promising	1684:1692	arg1	therapy					1706:1712	a promising alternative therapy	1682:1712	a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases	1682:1795	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	8	61	theme	process	1413:1419	arg1	mediators					1383:1391	important mediators	1373:1391	important mediators	1373:1391	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	61	theme	process	1413:1419	arg1	NO					1430:1431	NO	1430:1431	NO	1430:1431	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	61	theme	process	1413:1419	arg1	TNF-α					1439:1443	TNF-α	1439:1443	TNF-α	1439:1443	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	61	theme	process	1413:1419	arg1	IL-6					1450:1453	IL-6	1450:1453	IL-6	1450:1453	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	61	theme	process	1413:1419	arg1	O2-					1434:1436	O2-	1434:1436	O2-	1434:1436	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	8	62	theme	inflammatory	1400:1411	arg1	process					1413:1419	the inflammatory process	1396:1419	the inflammatory process	1396:1419	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	0	63	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	2	64	theme	chemical	314:321	arg1	composition					323:333	The chemical composition	310:333	The chemical composition of the extracts	310:349	The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer.					
33452968	10	65	theme	alternative	1694:1704	arg1	therapy					1706:1712	a promising alternative therapy	1682:1712	a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases	1682:1795	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	1	66	theme	stems	227:231	arg1	activity					186:193	the anti-inflammatory activity	164:193	the anti-inflammatory activity	164:193	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	1	66	theme	stems	227:231	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	4	67	theme	tumor	725:729	arg1	TNF-α					748:752	TNF-α	748:752	TNF-α	748:752	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	67	theme	tumor	725:729	arg1	cytokines					715:723	the pro-inflammatory cytokines	694:723	the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	694:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	67	theme	tumor	725:729	arg1	factor					740:745	tumor necrosis factor	725:745	tumor necrosis factor (TNF-α)	725:753	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	68	theme	necrosis	731:738	arg1	TNF-α					748:752	TNF-α	748:752	TNF-α	748:752	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	68	theme	necrosis	731:738	arg1	cytokines					715:723	the pro-inflammatory cytokines	694:723	the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	694:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	4	68	theme	necrosis	731:738	arg1	factor					740:745	tumor necrosis factor	725:745	tumor necrosis factor (TNF-α)	725:753	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	7	69	theme	fatty	1194:1198	arg1	alcohols					1200:1207	two fatty alcohols	1190:1207	two fatty alcohols	1190:1207	Eight compounds were tentatively identified in the R. petasites extracts, suggesting five diarylheptanoids, one flavonoid, and two fatty alcohols.					
33452968	10	70	theme	osteoarthritis	1750:1763	arg1	management					1736:1745	management	1736:1745	management	1736:1745	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	10	70	theme	osteoarthritis	1750:1763	arg1	treatment					1722:1730	treatment	1722:1730	treatment	1722:1730	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	10	71	dep	treatment	1722:1730	arg1	the					1718:1720	the	1718:1720	the	1718:1720	The results evidenced that R. petasites can be considered a promising alternative therapy for the treatment and management of osteoarthritis and other inflammatory diseases.					
33452968	8	72	theme	free	1281:1284	arg1	radicals					1286:1293	free radicals	1281:1293	free radicals	1281:1293	The in vitro results showed that the extracts were capable of blocking free radicals and/or inhibiting their intracellular actions by inhibiting the production of important mediators of the inflammatory process, such as NO, O2-, TNF-α, and IL-6.					
33452968	2	73	theme	linear	374:379	arg1	spectrometer					396:407	a linear iron trap mass spectrometer	372:407	a linear iron trap mass spectrometer	372:407	The chemical composition of the extracts was characterized in a linear iron trap mass spectrometer.					
33452968	4	74	theme	anion	658:662	arg1	production					633:642	the production	629:642	the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6)	629:778	In vitro anti-inflammatory activity was investigated in lipopolysaccharide-stimulated macrophages evaluating the influence on the production of superoxide anion (O2-), nitric oxide (NO), and the pro-inflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-6 (IL-6).					
33452968	0	75	theme	chemical	52:59	arg1	composition					61:71	chemical composition	52:71	chemical composition	52:71	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	0	76	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo anti-inflammatory activity and chemical composition of Renealmia petasites Gagnep.					
33452968	1	77	theme	chemical	139:146	arg1	composition					148:158	chemical composition	139:158	chemical composition	139:158	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33452968	1	78	theme	leaf	238:241	arg1	extracts					243:250	leaf extracts	238:250	leaf extracts	238:250	The study aimed to investigate the chemical composition and the anti-inflammatory activity of the hydroethanolic rhizomes, stems, and leaf extracts of Renealmia petasites using in vitro and in vivo assays.					
33307483	0	0	theme	hydrogen	87:94	arg1	peroxide-acetic					96:110	hydrogen peroxide-acetic	87:110	hydrogen peroxide-acetic	87:110	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	1	1	theme	obstacles	162:170	arg1	one					146:148	one	146:148	one	146:148	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	1	1	theme	obstacles	162:170	arg1	obstacles					162:170	the main obstacles	153:170	the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC)	153:282	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	5	2	from	yield	875:879	arg1	%					924:924	0.69% to 20.45%	910:924	0.69% to 20.45%	910:924	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	5	2	from	yield	875:879	arg1	%					965:965	5.76% to 92.89%	951:965	5.76% to 92.89%	951:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	2	3	theme	chemical	345:352	arg1	composition					354:364	chemical composition	345:364	chemical composition	345:364	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	6	4	theme	critical	1069:1076	arg1	role					1078:1081	a critical role	1067:1081	a critical role	1067:1081	This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.					
33307483	0	5	from	Effect	0:5	arg1	production					27:36	production	27:36	production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic	27:110	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	5	6	theme	monosaccharides	930:944	arg1	yield					875:879	the yield	871:879	the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89%	871:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	2	7	from	effects	303:309	arg1	composition					354:364	chemical composition	345:364	chemical composition	345:364	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	2	7	from	effects	303:309	arg1	features					378:385	structural features	367:385	structural features	367:385	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	2	7	from	effects	303:309	arg1	properties					397:406	physical properties	388:406	physical properties	388:406	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	2	7	from	effects	303:309	arg1	digestibility					422:434	enzymatic digestibility	412:434	enzymatic digestibility	412:434	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	6	8	theme	enzymatic	1135:1143	arg1	hydrolysis					1145:1154	enzymatic hydrolysis	1135:1154	enzymatic hydrolysis	1135:1154	This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.					
33307483	5	9	dep	%	914:914	arg1	to					916:917	to	916:917	to	916:917	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	3	10	theme	lignin	521:526	arg1	removal					528:534	the lignin removal	517:534	the lignin removal of HPAC-pretreated poplar	517:560	The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity.					
33307483	5	11	from	%	965:965	arg1	yield					875:879	the yield	871:879	the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89%	871:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	5	11	from	%	965:965	arg1	monosaccharides					930:944	monosaccharides	930:944	monosaccharides from 5.76% to 92.89%	930:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	5	11	from	%	965:965	arg1	xylooligosaccharides					884:903	xylooligosaccharides	884:903	xylooligosaccharides from 0.69% to 20.45%	884:924	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	4	12	theme	5 FPU/g	729:735	arg1	DM					737:738	5 FPU/g DM	729:738	5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L)	729:822	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	6	13	theme	poplar	1111:1116	arg1	carbohydrates					1118:1130	poplar carbohydrates	1111:1130	poplar carbohydrates	1111:1130	This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.					
33307483	3	14	theme	poplar	555:560	arg1	removal					528:534	the lignin removal	517:534	the lignin removal of HPAC-pretreated poplar	517:560	The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity.					
33307483	5	15	theme	0.69	910:913	arg1	%					914:914	%	914:914	%	914:914	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	1	16	theme	enzymatic	176:184	arg1	hydrolysis					186:195	enzymatic hydrolysis	176:195	enzymatic hydrolysis	176:195	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	4	17	theme	satisfying	636:645	arg1	yield					655:659	A satisfying glucose yield	634:659	A satisfying glucose yield (91.84%)	634:668	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	4	17	theme	satisfying	636:645	arg1	%					667:667	91.84%	662:667	91.84%	662:667	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	2	18	theme	physical	388:395	arg1	properties					397:406	physical properties	388:406	physical properties	388:406	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	5	19	theme	92.89	960:964	arg1	%					955:955	%	955:955	%	955:955	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	3	20	theme	HPAC-pretreated	539:553	arg1	poplar					555:560	HPAC-pretreated poplar	539:560	HPAC-pretreated poplar	539:560	The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity.					
33307483	4	21	theme	cellulase	743:751	arg1	loading					753:759	cellulase loading	743:759	cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L)	743:822	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	4	22	theme	pretreated	693:702	arg1	poplar					704:709	HPAC pretreated poplar	688:709	HPAC pretreated poplar (100 mM H2SO4)	688:724	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	4	22	theme	pretreated	693:702	arg1	H2SO4					719:723	100 mM H2SO4	712:723	100 mM H2SO4	712:723	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	2	23	theme	poplar	455:460	arg1	composition					354:364	chemical composition	345:364	chemical composition	345:364	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	2	23	theme	poplar	455:460	arg1	features					378:385	structural features	367:385	structural features	367:385	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	2	23	theme	poplar	455:460	arg1	properties					397:406	physical properties	388:406	physical properties	388:406	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	2	23	theme	poplar	455:460	arg1	digestibility					422:434	enzymatic digestibility	412:434	enzymatic digestibility	412:434	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	5	24	theme	20.45	919:923	arg1	%					914:914	%	914:914	%	914:914	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	4	25	theme	HPAC	688:691	arg1	poplar					704:709	HPAC pretreated poplar	688:709	HPAC pretreated poplar (100 mM H2SO4)	688:724	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	4	25	theme	HPAC	688:691	arg1	H2SO4					719:723	100 mM H2SO4	712:723	100 mM H2SO4	712:723	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	0	26	theme	acid	19:22	arg1	Effect					0:5	Effect	0:5	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic	0:110	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	2	27	theme	HPAC	439:442	arg1	poplar					455:460	HPAC pretreated poplar	439:460	HPAC pretreated poplar	439:460	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	4	28	theme	xylanase	782:789	arg1	addition					770:777	the addition	766:777	the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L)	766:822	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	3	29	theme	increased	580:588	arg1	accessibility					590:602	the increased accessibility	576:602	the increased accessibility	576:602	The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity.					
33307483	3	30	theme	increased	485:493	arg1	dosage					501:506	The increased H2SO4 dosage	481:506	The increased H2SO4 dosage	481:506	The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity.					
33307483	5	31	dep	%	955:955	arg1	to					957:958	to	957:958	to	957:958	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	4	32	theme	Tween	807:811	arg1	addition					770:777	the addition	766:777	the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L)	766:822	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	5	33	theme	concentration	848:860	arg1	increment					829:837	The increment	825:837	The increment of H2SO4 concentration	825:860	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	2	34	theme	enzymatic	412:420	arg1	digestibility					422:434	enzymatic digestibility	412:434	enzymatic digestibility	412:434	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	5	35	theme	H2SO4	842:846	arg1	concentration					848:860	H2SO4 concentration	842:860	H2SO4 concentration	842:860	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	2	36	theme	pretreated	444:453	arg1	poplar					455:460	HPAC pretreated poplar	439:460	HPAC pretreated poplar	439:460	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	6	37	theme	HPAC	1042:1045	arg1	pretreatment					1047:1058	HPAC pretreatment	1042:1058	HPAC pretreatment	1042:1058	This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.					
33307483	0	38	theme	xylooligosaccharides	41:60	arg1	production					27:36	production	27:36	production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic	27:110	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	4	39	with	loading	753:759	arg1	addition					770:777	the addition	766:777	the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L)	766:822	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	5	40	theme	enzymatic	992:1000	arg1	hydrolysis					1002:1011	two-step enzymatic hydrolysis	983:1011	two-step enzymatic hydrolysis	983:1011	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	5	41	theme	xylooligosaccharides	884:903	arg1	yield					875:879	the yield	871:879	the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89%	871:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	3	42	theme	H2SO4	495:499	arg1	dosage					501:506	The increased H2SO4 dosage	481:506	The increased H2SO4 dosage	481:506	The increased H2SO4 dosage enhanced the lignin removal of HPAC-pretreated poplar, resulting in the increased accessibility and decreased hydrophobicity.					
33307483	2	43	theme	concentration	328:340	arg1	effects					303:309	the effects	299:309	the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar	299:460	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	5	44	from	%	924:924	arg1	yield					875:879	the yield	871:879	the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89%	871:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	5	44	from	%	924:924	arg1	monosaccharides					930:944	monosaccharides	930:944	monosaccharides from 5.76% to 92.89%	930:965	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	5	44	from	%	924:924	arg1	xylooligosaccharides					884:903	xylooligosaccharides	884:903	xylooligosaccharides from 0.69% to 20.45%	884:924	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	4	45	theme	30 U/g	792:797	arg1	DM					799:800	30 U/g DM	792:800	30 U/g DM	792:800	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	4	45	theme	30 U/g	792:797	arg1	xylanase					782:789	xylanase	782:789	xylanase (30 U/g DM)	782:801	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	5	46	theme	two-step	983:990	arg1	hydrolysis					1002:1011	two-step enzymatic hydrolysis	983:1011	two-step enzymatic hydrolysis	983:1011	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	2	47	theme	acid	323:326	arg1	concentration					328:340	sulfuric acid concentration	314:340	sulfuric acid concentration	314:340	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	6	48	theme	efficient	1086:1094	arg1	utilization					1096:1106	efficient utilization	1086:1106	efficient utilization of poplar carbohydrates by enzymatic hydrolysis	1086:1154	This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.					
33307483	4	49	theme	glucose	647:653	arg1	yield					655:659	A satisfying glucose yield	634:659	A satisfying glucose yield (91.84%)	634:668	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	4	49	theme	glucose	647:653	arg1	%					667:667	91.84%	662:667	91.84%	662:667	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	5	50	theme	5.76	951:954	arg1	%					955:955	%	955:955	%	955:955	The increment of H2SO4 concentration promoted the yield of xylooligosaccharides from 0.69% to 20.45% and monosaccharides from 5.76% to 92.89% respectively by two-step enzymatic hydrolysis.					
33307483	4	51	theme	loading	753:759	arg1	DM					737:738	5 FPU/g DM	729:738	5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L)	729:822	A satisfying glucose yield (91.84%) was obtained from HPAC pretreated poplar (100 mM H2SO4) at 5 FPU/g DM of cellulase loading with the addition of xylanase (30 U/g DM) and Tween 80 (3 g/L).					
33307483	2	52	theme	sulfuric	314:321	arg1	concentration					328:340	sulfuric acid concentration	314:340	sulfuric acid concentration	314:340	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	1	53	theme	hydrogen	234:241	arg1	HPAC					278:281	HPAC	278:281	HPAC	278:281	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	1	53	theme	hydrogen	234:241	arg1	pretreatment					264:275	hydrogen peroxide-acetic acid pretreatment	234:275	hydrogen peroxide-acetic acid pretreatment (HPAC)	234:282	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	0	54	theme	monosaccharides	66:80	arg1	production					27:36	production	27:36	production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic	27:110	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	0	55	from	peroxide-acetic	96:110	arg1	production					27:36	production	27:36	production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic	27:110	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	2	56	theme	structural	367:376	arg1	features					378:385	structural features	367:385	structural features	367:385	In this work, the effects of sulfuric acid concentration on chemical composition, structural features, physical properties and enzymatic digestibility of HPAC pretreated poplar were investigated.					
33307483	1	57	theme	peroxide-acetic	243:257	arg1	HPAC					278:281	HPAC	278:281	HPAC	278:281	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	1	57	theme	peroxide-acetic	243:257	arg1	pretreatment					264:275	hydrogen peroxide-acetic acid pretreatment	234:275	hydrogen peroxide-acetic acid pretreatment (HPAC)	234:282	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	6	58	theme	carbohydrates	1118:1130	arg1	utilization					1096:1106	efficient utilization	1086:1106	efficient utilization of poplar carbohydrates by enzymatic hydrolysis	1086:1154	This work demonstrated that HPAC pretreatment played a critical role in efficient utilization of poplar carbohydrates by enzymatic hydrolysis.					
33307483	0	59	theme	sulfuric	10:17	arg1	acid					19:22	sulfuric acid	10:22	sulfuric acid	10:22	Effect of sulfuric acid on production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar.					
33307483	1	60	theme	main	157:160	arg1	obstacles					162:170	the main obstacles	153:170	the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC)	153:282	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	1	61	theme	acid	259:262	arg1	HPAC					278:281	HPAC	278:281	HPAC	278:281	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33307483	1	61	theme	acid	259:262	arg1	pretreatment					264:275	hydrogen peroxide-acetic acid pretreatment	234:275	hydrogen peroxide-acetic acid pretreatment (HPAC)	234:282	Lignin is one of the main obstacles for enzymatic hydrolysis, which can be selectively removed by hydrogen peroxide-acetic acid pretreatment (HPAC).					
33651584	0	0	theme	Biomolecular	105:116	arg1	Conjugation					118:128	Programmable Biomolecular Conjugation	92:128	Programmable Biomolecular Conjugation	92:128	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	7	1	theme	substantial	1386:1396	arg1	potential					1398:1406	substantial potential	1386:1406	substantial potential	1386:1406	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	7	2	theme	significant	1260:1270	arg1	step					1272:1275	a significant step	1258:1275	a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications	1258:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	6	3	theme	compositions	1189:1200	arg1	tuning					1168:1173	simple tuning	1161:1173	simple tuning of prepolymer compositions for the shell layer	1161:1220	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	2	4	theme	-based	371:376	arg1	microspheres					389:400	poly(ethylene glycol)-based core-shell microspheres	350:400	poly(ethylene glycol)-based core-shell microspheres	350:400	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	5	5	theme	biomolecules	828:839	arg1	conjugation					813:823	Selective conjugation	803:823	Selective conjugation of biomolecules	803:839	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	6	6	theme	diffusion	1139:1147	arg1	barriers					1149:1156	tunable size-selective diffusion barriers	1116:1156	tunable size-selective diffusion barriers	1116:1156	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	0	7	theme	Programmable	92:103	arg1	Conjugation					118:128	Programmable Biomolecular Conjugation	92:128	Programmable Biomolecular Conjugation	92:128	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	3	8	theme	varying	558:564	arg1	dimensions					566:575	varying dimensions	558:575	varying dimensions of each compartment	558:595	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	6	9	theme	size-selective	1124:1137	arg1	barriers					1149:1156	tunable size-selective diffusion barriers	1116:1156	tunable size-selective diffusion barriers	1116:1156	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	5	10	theme	Selective	803:811	arg1	conjugation					813:823	Selective conjugation	803:823	Selective conjugation of biomolecules	803:839	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	6	11	theme	kinetics	1014:1021	arg1	studies					1023:1029	in-depth protein conjugation kinetics studies	985:1029	in-depth protein conjugation kinetics studies using microspheres with varying shell porosities	985:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	5	12	with	reaction	882:889	arg1	groups					907:912	functional groups	896:912	functional groups in the core compartment with minimal non-specific adsorption	896:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	6	13	theme	tunable	1116:1122	arg1	barriers					1149:1156	tunable size-selective diffusion barriers	1116:1156	tunable size-selective diffusion barriers	1116:1156	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	6	14	theme	conjugation	1002:1012	arg1	studies					1023:1029	in-depth protein conjugation kinetics studies	985:1029	in-depth protein conjugation kinetics studies using microspheres with varying shell porosities	985:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	7	15	theme	programmable	1289:1300	arg1	fabrication					1318:1328	programmable high-throughput fabrication	1289:1328	programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications	1289:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	7	16	theme	forward	1277:1283	arg1	step					1272:1275	a significant step	1258:1275	a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications	1258:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	1	17	theme	rapid	154:158	arg1	approach					173:180	a simple and rapid microfluidic approach	141:180	a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step	141:241	We report a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step.					
33651584	7	18	theme	microspheres	1358:1369	arg1	fabrication					1318:1328	programmable high-throughput fabrication	1289:1328	programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications	1289:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	4	19	theme	shell	790:794	arg1	layer					796:800	the shell layer	786:800	the shell layer	786:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	5	20	theme	functional	896:905	arg1	groups					907:912	functional groups	896:912	functional groups in the core compartment with minimal non-specific adsorption	896:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	1	21	theme	core-shell	193:202	arg1	microspheres					213:224	core-shell hydrogel microspheres	193:224	core-shell hydrogel microspheres	193:224	We report a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step.					
33651584	0	22	theme	Core-Shell	55:64	arg1	Microspheres					75:86	Core-Shell Hydrogel Microspheres	55:86	Core-Shell Hydrogel Microspheres	55:86	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	6	23	theme	in-depth	985:992	arg1	studies					1023:1029	in-depth protein conjugation kinetics studies	985:1029	in-depth protein conjugation kinetics studies using microspheres with varying shell porosities	985:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	7	24	theme	hydrogel	1349:1356	arg1	microspheres					1358:1369	multifunctional hydrogel microspheres	1333:1369	multifunctional hydrogel microspheres	1333:1369	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	5	25	with	compartment	926:936	arg1	adsorption					964:973	minimal non-specific adsorption	943:973	minimal non-specific adsorption	943:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	3	26	theme	compartment	585:595	arg1	dimensions					566:575	varying dimensions	558:575	varying dimensions of each compartment	558:595	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	1	27	theme	hydrogel	204:211	arg1	microspheres					213:224	core-shell hydrogel microspheres	193:224	core-shell hydrogel microspheres	193:224	We report a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step.					
33651584	0	28	theme	Emulsion-Based	7:20	arg1	Production					41:50	Triple Emulsion-Based Rapid Microfluidic Production	0:50	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.	0:129	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	5	29	from	groups	907:912	arg1	compartment					926:936	the core compartment	917:936	the core compartment with minimal non-specific adsorption	917:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	7	30	theme	biomedical	1428:1437	arg1	applications					1455:1466	biomedical and biochemical applications	1428:1466	biomedical and biochemical applications	1428:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	3	31	theme	controlled	628:637	arg1	rates					644:648	independently and precisely controlled flow rates	600:648	independently and precisely controlled flow rates	600:648	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	6	32	theme	protein	994:1000	arg1	studies					1023:1029	in-depth protein conjugation kinetics studies	985:1029	in-depth protein conjugation kinetics studies using microspheres with varying shell porosities	985:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	6	33	theme	shell	1210:1214	arg1	layer					1216:1220	the shell layer	1206:1220	the shell layer	1206:1220	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	0	34	theme	Triple	0:5	arg1	Production					41:50	Triple Emulsion-Based Rapid Microfluidic Production	0:50	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.	0:129	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	7	35	theme	high-throughput	1302:1316	arg1	fabrication					1318:1328	programmable high-throughput fabrication	1289:1328	programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications	1289:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	3	36	with	microspheres	540:551	arg1	dimensions					566:575	varying dimensions	558:575	varying dimensions of each compartment	558:595	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	5	37	theme	non-specific	951:962	arg1	adsorption					964:973	minimal non-specific adsorption	943:973	minimal non-specific adsorption	943:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	3	38	theme	flow	639:642	arg1	rates					644:648	independently and precisely controlled flow rates	600:648	independently and precisely controlled flow rates	600:648	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	4	39	theme	core	728:731	arg1	compartment					733:743	the core compartment	724:743	the core compartment with negligible cross-contamination into the shell layer	724:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	0	40	theme	Microfluidic	28:39	arg1	Production					41:50	Triple Emulsion-Based Rapid Microfluidic Production	0:50	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.	0:129	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	5	41	theme	core	921:924	arg1	compartment					926:936	the core compartment	917:936	the core compartment with minimal non-specific adsorption	917:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	2	42	theme	oil	465:467	arg1	layer					469:473	the oil layer	461:473	the oil layer	461:473	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	4	43	with	compartment	733:743	arg1	cross-contamination					761:779	negligible cross-contamination	750:779	negligible cross-contamination into the shell layer	750:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	2	44	theme	prepolymer	323:332	arg1	phases					334:339	two prepolymer phases	319:339	two prepolymer phases	319:339	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	1	45	theme	single	231:236	arg1	step					238:241	a single step	229:241	a single step	229:241	We report a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step.					
33651584	5	46	theme	rapid	862:866	arg1	reaction					882:889	a rapid bioorthogonal reaction	860:889	a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption	860:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	2	47	theme	ethylene	355:362	arg1	poly					350:353	poly	350:353	poly(ethylene glycol)-based core-shell microspheres	350:400	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	2	47	theme	ethylene	355:362	arg1	glycol					364:369	ethylene glycol	355:369	ethylene glycol	355:369	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	6	48	theme	prepolymer	1178:1187	arg1	compositions					1189:1200	prepolymer compositions	1178:1200	prepolymer compositions	1178:1200	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	2	49	theme	emulsion	262:269	arg1	drops					271:275	triple emulsion drops	255:275	triple emulsion drops with sacrificial oil layers that separate two prepolymer phases	255:339	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	4	50	from	incorporation	684:696	arg1	compartment					733:743	the core compartment	724:743	the core compartment with negligible cross-contamination into the shell layer	724:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	4	51	theme	moieties	712:719	arg1	incorporation					684:696	stable and uniform incorporation	665:696	stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer	665:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	4	52	theme	negligible	750:759	arg1	cross-contamination					761:779	negligible cross-contamination	750:779	negligible cross-contamination into the shell layer	750:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	5	53	theme	bioorthogonal	868:880	arg1	reaction					882:889	a rapid bioorthogonal reaction	860:889	a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption	860:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	2	54	theme	triple	255:260	arg1	drops					271:275	triple emulsion drops	255:275	triple emulsion drops with sacrificial oil layers that separate two prepolymer phases	255:339	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	5	55	theme	minimal	943:949	arg1	adsorption					964:973	minimal non-specific adsorption	943:973	minimal non-specific adsorption	943:973	Selective conjugation of biomolecules is enabled through a rapid bioorthogonal reaction with functional groups in the core compartment with minimal non-specific adsorption.					
33651584	2	56	theme	spontaneous	438:448	arg1	removal					450:456	spontaneous removal	438:456	spontaneous removal of the oil layer	438:473	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	0	57	theme	Hydrogel	66:73	arg1	Microspheres					75:86	Core-Shell Hydrogel Microspheres	55:86	Core-Shell Hydrogel Microspheres	55:86	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	2	58	with	drops	271:275	arg1	layers					298:303	sacrificial oil layers	282:303	sacrificial oil layers that separate two prepolymer phases	282:339	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	7	59	theme	applications	1455:1466	arg1	array					1419:1423	a large array	1411:1423	a large array of biomedical and biochemical applications	1411:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	3	60	theme	core-shell	529:538	arg1	microspheres					540:551	monodisperse core-shell microspheres	516:551	monodisperse core-shell microspheres with varying dimensions of each compartment	516:595	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	2	61	theme	layer	469:473	arg1	removal					450:456	spontaneous removal	438:456	spontaneous removal of the oil layer	438:473	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	6	62	theme	varying	1055:1061	arg1	porosities					1069:1078	varying shell porosities	1055:1078	varying shell porosities	1055:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	7	63	contain	possess	1378:1384	arg1	microspheres					1358:1369	multifunctional hydrogel microspheres	1333:1369	multifunctional hydrogel microspheres	1333:1369	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	7	63	contain	possess	1378:1384	arg2	potential					1398:1406	substantial potential	1386:1406	substantial potential	1386:1406	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	1	64	theme	simple	143:148	arg1	approach					173:180	a simple and rapid microfluidic approach	141:180	a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step	141:241	We report a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step.					
33651584	7	65	theme	large	1413:1417	arg1	array					1419:1423	a large array	1411:1423	a large array of biomedical and biochemical applications	1411:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	7	66	theme	multifunctional	1333:1347	arg1	microspheres					1358:1369	multifunctional hydrogel microspheres	1333:1369	multifunctional hydrogel microspheres	1333:1369	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	2	67	theme	oil	294:296	arg1	layers					298:303	sacrificial oil layers	282:303	sacrificial oil layers that separate two prepolymer phases	282:339	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	6	68	with	microspheres	1037:1048	arg1	porosities					1069:1078	varying shell porosities	1055:1078	varying shell porosities	1055:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	4	69	theme	stable	665:670	arg1	incorporation					684:696	stable and uniform incorporation	665:696	stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer	665:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	7	70	theme	biochemical	1443:1453	arg1	applications					1455:1466	biomedical and biochemical applications	1428:1466	biomedical and biochemical applications	1428:1466	Combined, these results illustrate a significant step forward for programmable high-throughput fabrication of multifunctional hydrogel microspheres, which possess substantial potential in a large array of biomedical and biochemical applications.					
33651584	2	71	theme	sacrificial	282:292	arg1	layers					298:303	sacrificial oil layers	282:303	sacrificial oil layers that separate two prepolymer phases	282:339	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	2	72	theme	poly	350:353	arg1	microspheres					389:400	poly(ethylene glycol)-based core-shell microspheres	350:400	poly(ethylene glycol)-based core-shell microspheres	350:400	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	6	73	theme	simple	1161:1166	arg1	tuning					1168:1173	simple tuning	1161:1173	simple tuning of prepolymer compositions for the shell layer	1161:1220	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
33651584	4	74	theme	functional	701:710	arg1	moieties					712:719	functional moieties	701:719	functional moieties	701:719	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	1	75	theme	microfluidic	160:171	arg1	approach					173:180	a simple and rapid microfluidic approach	141:180	a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step	141:241	We report a simple and rapid microfluidic approach to produce core-shell hydrogel microspheres in a single step.					
33651584	3	76	theme	monodisperse	516:527	arg1	microspheres					540:551	monodisperse core-shell microspheres	516:551	monodisperse core-shell microspheres with varying dimensions of each compartment	516:595	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	4	77	theme	uniform	676:682	arg1	incorporation					684:696	stable and uniform incorporation	665:696	stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer	665:800	This leads to stable and uniform incorporation of functional moieties in the core compartment with negligible cross-contamination into the shell layer.					
33651584	0	78	theme	Microspheres	75:86	arg1	Production					41:50	Triple Emulsion-Based Rapid Microfluidic Production	0:50	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.	0:129	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	0	79	theme	Rapid	22:26	arg1	Production					41:50	Triple Emulsion-Based Rapid Microfluidic Production	0:50	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.	0:129	Triple Emulsion-Based Rapid Microfluidic Production of Core-Shell Hydrogel Microspheres for Programmable Biomolecular Conjugation.					
33651584	2	80	theme	core-shell	378:387	arg1	microspheres					389:400	poly(ethylene glycol)-based core-shell microspheres	350:400	poly(ethylene glycol)-based core-shell microspheres	350:400	We exploit triple emulsion drops with sacrificial oil layers that separate two prepolymer phases, forming poly(ethylene glycol)-based core-shell microspheres via photopolymerization followed by spontaneous removal of the oil layer.					
33651584	3	81	theme	microspheres	540:551	arg1	production					502:511	the production	498:511	the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates	498:648	Our technique enables the production of monodisperse core-shell microspheres with varying dimensions of each compartment by independently and precisely controlled flow rates.					
33651584	6	82	theme	shell	1063:1067	arg1	porosities					1069:1078	varying shell porosities	1055:1078	varying shell porosities	1055:1078	Finally, in-depth protein conjugation kinetics studies using microspheres with varying shell porosities highlight the capability to provide tunable size-selective diffusion barriers by simple tuning of prepolymer compositions for the shell layer.					
31978201	10	0	from	contents	1381:1388	arg1	honeydew					1431:1438	the honeydew	1427:1438	the honeydew	1427:1438	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	6	1	theme	different	821:829	arg1	proportions					831:841	different proportions	821:841	different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides	821:917	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	8	2	theme	whole-body	1177:1186	arg1	homogenates					1188:1198	whole-body homogenates	1177:1198	whole-body homogenates of aphids	1177:1208	Sucrose hydrolysis and oligosaccharide formation was shown in whole-body homogenates of aphids.					
31978201	11	3	theme	phloem	1595:1600	arg1	exudates					1602:1609	the phloem exudates	1591:1609	the phloem exudates of both tree species	1591:1630	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	11	4	theme	amino	1521:1525	arg1	acids					1527:1531	predominant amino acids	1509:1531	predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species	1509:1630	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	3	5	theme	zoological	483:492	arg1	species					494:500	those botanical and zoological species	463:500	those botanical and zoological species from which the majority of honeydew honey originates	463:553	This is particularly relevant for those botanical and zoological species from which the majority of honeydew honey originates.					
31978201	4	6	from	species	608:614	arg1	honeydew					583:590	honeydew	583:590	honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies	583:714	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	9	7	from	aphid-extracts	1260:1273	arg1	type					1215:1218	The type	1211:1218	The type of the produced oligosaccharides in the aphid-extracts	1211:1273	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	6	8	theme	species	803:809	arg1	Honeydew					787:794	Honeydew	787:794	Honeydew of all species	787:809	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	6	9	theme	further	888:894	arg1	di-					896:898	further di-	888:898	further di-	888:898	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	1	10	theme	phloem	174:179	arg1	sap					181:183	the phloem sap	170:183	the phloem sap of plants	170:193	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	0	11	theme	Picea	122:126	arg1	abies					128:132	Picea abies	122:132	Picea abies	122:132	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	3	12	theme	honey	538:542	arg1	majority					517:524	the majority	513:524	the majority of honeydew honey	513:542	This is particularly relevant for those botanical and zoological species from which the majority of honeydew honey originates.					
31978201	0	13	from	species	88:94	arg1	Sugar					0:4	Sugar	0:4	Sugar	0:4	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	0	13	from	species	88:94	arg1	acid					13:16	amino acid	7:16	amino acid	7:16	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	0	13	from	species	88:94	arg1	profiling					36:44	inorganic ion profiling	22:44	inorganic ion profiling	22:44	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	11	14	from	acids	1527:1531	arg1	honeydew					1540:1547	the honeydew	1536:1547	the honeydew of all six hemipteran species	1536:1577	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	11	14	from	acids	1527:1531	arg1	exudates					1602:1609	the phloem exudates	1591:1609	the phloem exudates of both tree species	1591:1630	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	12	15	theme	inorganic	1660:1668	arg1	ion					1670:1672	the dominant inorganic ion	1647:1672	the dominant inorganic ion in all honeydew samples and also in the phloem exudate	1647:1727	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	12	15	theme	inorganic	1660:1668	arg1	Potassium					1633:1641	Potassium	1633:1641	Potassium	1633:1641	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	13	16	theme	host	1854:1857	arg1	plant					1859:1863	the host plant	1850:1863	the host plant	1850:1863	Statistical analyses reveal that the sugar composition of honeydew is determined more by the hemipteran species than by the host plant.					
31978201	0	17	theme	amino	7:11	arg1	acid					13:16	amino acid	7:16	amino acid	7:16	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	0	18	theme	honeydew	53:60	arg1	Sugar					0:4	Sugar	0:4	Sugar	0:4	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	0	18	theme	honeydew	53:60	arg1	acid					13:16	amino acid	7:16	amino acid	7:16	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	0	18	theme	honeydew	53:60	arg1	profiling					36:44	inorganic ion profiling	22:44	inorganic ion profiling	22:44	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	9	19	theme	produced	1227:1234	arg1	oligosaccharides					1236:1251	the produced oligosaccharides	1223:1251	the produced oligosaccharides in the aphid-extracts	1223:1273	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	1	20	theme	Several	135:141	arg1	species					154:160	Several hemipteran species	135:160	Several hemipteran species	135:160	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	10	21	theme	amino	1393:1397	arg1	acids					1399:1403	amino acids	1393:1403	amino acids	1393:1403	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	0	22	theme	different	67:75	arg1	species					88:94	different hemipteran species	67:94	different hemipteran species feeding on Abies alba and Picea abies	67:132	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	14	23	theme	honey	1937:1941	arg1	composition					1913:1923	the sugar composition	1903:1923	the sugar composition of honeydew honey	1903:1941	Consequently, it can be assumed that the sugar composition of honeydew honey is also more influenced by the hemipteran species than by the host tree.					
31978201	9	24	from	composition	1311:1321	arg1	honeydew					1330:1337	the honeydew	1326:1337	the honeydew of the different aphid species	1326:1368	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	4	25	from	species	685:691	arg1	honeydew					583:590	honeydew	583:590	honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies	583:714	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	6	26	contain	contained	966:974	arg2	trisaccharides					979:992	no trisaccharides	976:992	no trisaccharides	976:992	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	6	26	contain	contained	966:974	arg1	exudates					939:946	the phloem exudates	928:946	the phloem exudates of the host trees	928:964	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	10	27	theme	ions	1419:1422	arg1	lower					1450:1454	lower	1450:1454	lower	1450:1454	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	10	27	theme	ions	1419:1422	arg1	contents					1381:1388	The total contents	1371:1388	The total contents of amino acids and inorganic ions in the honeydew	1371:1438	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	14	28	theme	sugar	1907:1911	arg1	composition					1913:1923	the sugar composition	1903:1923	the sugar composition of honeydew honey	1903:1941	Consequently, it can be assumed that the sugar composition of honeydew honey is also more influenced by the hemipteran species than by the host tree.					
31978201	2	29	theme	host	379:382	arg1	plant					384:388	the host plant	375:388	the host plant	375:388	Therefore, it is important to know whether it is predominantly the hemipteran species or the host plant to influence the honeydew composition.					
31978201	13	30	theme	sugar	1767:1771	arg1	composition					1773:1783	the sugar composition	1763:1783	the sugar composition of honeydew	1763:1795	Statistical analyses reveal that the sugar composition of honeydew is determined more by the hemipteran species than by the host plant.					
31978201	7	31	theme	same	1096:1099	arg1	species					1106:1112	the same tree species	1092:1112	the same tree species	1092:1112	Moreover, the proportions of sugars differed significantly between hemipteran species feeding on the same tree species.					
31978201	9	32	theme	oligosaccharide	1295:1309	arg1	composition					1311:1321	the oligosaccharide composition	1291:1321	the oligosaccharide composition in the honeydew of the different aphid species	1291:1368	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	6	33	theme	trees	960:964	arg1	exudates					939:946	the phloem exudates	928:946	the phloem exudates of the host trees	928:964	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	14	34	theme	hemipteran	1974:1983	arg1	species					1985:1991	the hemipteran species	1970:1991	the hemipteran species	1970:1991	Consequently, it can be assumed that the sugar composition of honeydew honey is also more influenced by the hemipteran species than by the host tree.					
31978201	2	35	theme	hemipteran	353:362	arg1	species					364:370	the hemipteran species	349:370	the hemipteran species	349:370	Therefore, it is important to know whether it is predominantly the hemipteran species or the host plant to influence the honeydew composition.					
31978201	2	35	theme	hemipteran	353:362	arg1	it					329:330	it	329:330	it	329:330	Therefore, it is important to know whether it is predominantly the hemipteran species or the host plant to influence the honeydew composition.					
31978201	12	36	from	ion	1670:1672	arg1	exudate					1721:1727	the phloem exudate	1710:1727	the phloem exudate	1710:1727	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	12	36	from	ion	1670:1672	arg1	samples					1690:1696	all honeydew samples	1677:1696	all honeydew samples	1677:1696	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	11	37	theme	species	1624:1630	arg1	exudates					1602:1609	the phloem exudates	1591:1609	the phloem exudates of both tree species	1591:1630	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	5	38	theme	Phloem	732:737	arg1	exudates					739:746	Phloem exudates	732:746	Phloem exudates of the host plants	732:765	Phloem exudates of the host plants were also analyzed.					
31978201	1	39	theme	honeydew	270:277	arg1	honey					279:283	honeydew honey	270:283	honeydew honey	270:283	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	1	40	theme	plants	188:193	arg1	sap					181:183	the phloem sap	170:183	the phloem sap of plants	170:193	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	5	41	theme	host	755:758	arg1	plants					760:765	the host plants	751:765	the host plants	751:765	Phloem exudates of the host plants were also analyzed.					
31978201	12	42	theme	phloem	1714:1719	arg1	exudate					1721:1727	the phloem exudate	1710:1727	the phloem exudate	1710:1727	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	13	43	theme	Statistical	1730:1740	arg1	analyses					1742:1749	Statistical analyses	1730:1749	Statistical analyses	1730:1749	Statistical analyses reveal that the sugar composition of honeydew is determined more by the hemipteran species than by the host plant.					
31978201	11	44	theme	species	1571:1577	arg1	honeydew					1540:1547	the honeydew	1536:1547	the honeydew of all six hemipteran species	1536:1577	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	9	45	theme	aphid	1356:1360	arg1	species					1362:1368	the different aphid species	1342:1368	the different aphid species	1342:1368	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	9	46	from	type	1215:1218	arg1	aphid-extracts					1260:1273	the aphid-extracts	1256:1273	the aphid-extracts	1256:1273	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	6	47	theme	sucrose	855:861	arg1	proportions					831:841	different proportions	821:841	different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides	821:917	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	8	48	theme	Sucrose	1115:1121	arg1	hydrolysis					1123:1132	Sucrose hydrolysis	1115:1132	Sucrose hydrolysis	1115:1132	Sucrose hydrolysis and oligosaccharide formation was shown in whole-body homogenates of aphids.					
31978201	6	49	theme	hexoses	846:852	arg1	proportions					831:841	different proportions	821:841	different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides	821:917	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	7	50	theme	sugars	1024:1029	arg1	proportions					1009:1019	the proportions	1005:1019	the proportions of sugars	1005:1029	Moreover, the proportions of sugars differed significantly between hemipteran species feeding on the same tree species.					
31978201	1	51	theme	honeydew	224:231	arg1	honeydew					224:231	honeydew	224:231	honeydew	224:231	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	1	51	theme	honeydew	224:231	arg1	amounts					213:219	large amounts	207:219	large amounts of honeydew that is collected by bees to produce honeydew honey	207:283	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	1	52	theme	hemipteran	143:152	arg1	species					154:160	Several hemipteran species	135:160	Several hemipteran species	135:160	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	0	53	theme	ion	32:34	arg1	profiling					36:44	inorganic ion profiling	22:44	inorganic ion profiling	22:44	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	4	54	theme	Cinara	601:606	arg1	species					608:614	two Cinara species	597:614	two Cinara species located on Abies alba	597:636	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	10	55	theme	total	1375:1379	arg1	lower					1450:1454	lower	1450:1454	lower	1450:1454	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	10	55	theme	total	1375:1379	arg1	contents					1381:1388	The total contents	1371:1388	The total contents of amino acids and inorganic ions in the honeydew	1371:1438	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	12	56	theme	honeydew	1681:1688	arg1	samples					1690:1696	all honeydew samples	1677:1696	all honeydew samples	1677:1696	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	4	57	theme	located	616:622	arg1	species					608:614	two Cinara species	597:614	two Cinara species located on Abies alba	597:636	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	0	58	theme	Abies	107:111	arg1	alba					113:116	Abies alba	107:116	Abies alba	107:116	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	11	59	theme	predominant	1509:1519	arg1	acids					1527:1531	predominant amino acids	1509:1531	predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species	1509:1630	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	5	60	theme	plants	760:765	arg1	exudates					739:746	Phloem exudates	732:746	Phloem exudates of the host plants	732:765	Phloem exudates of the host plants were also analyzed.					
31978201	6	61	contain	contained	811:819	arg1	Honeydew					787:794	Honeydew	787:794	Honeydew of all species	787:809	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	6	61	contain	contained	811:819	arg2	proportions					831:841	different proportions	821:841	different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides	821:917	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	8	62	theme	oligosaccharide	1138:1152	arg1	formation					1154:1162	oligosaccharide formation	1138:1162	oligosaccharide formation	1138:1162	Sucrose hydrolysis and oligosaccharide formation was shown in whole-body homogenates of aphids.					
31978201	3	63	theme	honeydew	529:536	arg1	honey					538:542	honeydew honey	529:542	honeydew honey	529:542	This is particularly relevant for those botanical and zoological species from which the majority of honeydew honey originates.					
31978201	12	64	theme	dominant	1651:1658	arg1	ion					1670:1672	the dominant inorganic ion	1647:1672	the dominant inorganic ion in all honeydew samples and also in the phloem exudate	1647:1727	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	12	64	theme	dominant	1651:1658	arg1	Potassium					1633:1641	Potassium	1633:1641	Potassium	1633:1641	Potassium was the dominant inorganic ion in all honeydew samples and also in the phloem exudate.					
31978201	6	65	theme	phloem	932:937	arg1	exudates					939:946	the phloem exudates	928:946	the phloem exudates of the host trees	928:964	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	4	66	theme	Abies	627:631	arg1	alba					633:636	Abies alba	627:636	Abies alba	627:636	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	10	67	theme	acids	1399:1403	arg1	lower					1450:1454	lower	1450:1454	lower	1450:1454	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	10	67	theme	acids	1399:1403	arg1	contents					1381:1388	The total contents	1371:1388	The total contents of amino acids and inorganic ions in the honeydew	1371:1438	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	13	68	theme	hemipteran	1823:1832	arg1	species					1834:1840	the hemipteran species	1819:1840	the hemipteran species	1819:1840	Statistical analyses reveal that the sugar composition of honeydew is determined more by the hemipteran species than by the host plant.					
31978201	0	69	theme	hemipteran	77:86	arg1	species					88:94	different hemipteran species	67:94	different hemipteran species feeding on Abies alba and Picea abies	67:132	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	9	70	theme	oligosaccharides	1236:1251	arg1	type					1215:1218	The type	1211:1218	The type of the produced oligosaccharides in the aphid-extracts	1211:1273	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	6	71	theme	trisaccharides	904:917	arg1	proportions					831:841	different proportions	821:841	different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides	821:917	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	4	72	theme	located	693:699	arg1	Cinara					658:663	two Cinara and two Physokermes species	654:691	Cinara	658:663	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	13	73	theme	honeydew	1788:1795	arg1	composition					1773:1783	the sugar composition	1763:1783	the sugar composition of honeydew	1763:1795	Statistical analyses reveal that the sugar composition of honeydew is determined more by the hemipteran species than by the host plant.					
31978201	8	74	theme	aphids	1203:1208	arg1	homogenates					1188:1198	whole-body homogenates	1177:1198	whole-body homogenates of aphids	1177:1208	Sucrose hydrolysis and oligosaccharide formation was shown in whole-body homogenates of aphids.					
31978201	14	75	theme	honeydew	1928:1935	arg1	honey					1937:1941	honeydew honey	1928:1941	honeydew honey	1928:1941	Consequently, it can be assumed that the sugar composition of honeydew honey is also more influenced by the hemipteran species than by the host tree.					
31978201	14	76	theme	host	2005:2008	arg1	tree					2010:2013	the host tree	2001:2013	the host tree	2001:2013	Consequently, it can be assumed that the sugar composition of honeydew honey is also more influenced by the hemipteran species than by the host tree.					
31978201	4	77	theme	Picea	704:708	arg1	abies					710:714	Picea abies	704:714	Picea abies	704:714	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	7	78	theme	tree	1101:1104	arg1	species					1106:1112	the same tree species	1092:1112	the same tree species	1092:1112	Moreover, the proportions of sugars differed significantly between hemipteran species feeding on the same tree species.					
31978201	10	79	theme	inorganic	1409:1417	arg1	ions					1419:1422	inorganic ions	1409:1422	inorganic ions	1409:1422	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	4	80	theme	Physokermes	673:683	arg1	species					685:691	two Cinara and two Physokermes species	654:691	species	685:691	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	0	81	theme	inorganic	22:30	arg1	profiling					36:44	inorganic ion profiling	22:44	inorganic ion profiling	22:44	Sugar, amino acid and inorganic ion profiling of the honeydew from different hemipteran species feeding on Abies alba and Picea abies.					
31978201	6	82	theme	host	955:958	arg1	trees					960:964	the host trees	951:964	the host trees	951:964	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	2	83	theme	honeydew	407:414	arg1	composition					416:426	the honeydew composition	403:426	the honeydew composition	403:426	Therefore, it is important to know whether it is predominantly the hemipteran species or the host plant to influence the honeydew composition.					
31978201	9	84	from	oligosaccharides	1236:1251	arg1	aphid-extracts					1260:1273	the aphid-extracts	1256:1273	the aphid-extracts	1256:1273	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	11	85	theme	tree	1619:1622	arg1	species					1624:1630	both tree species	1614:1630	both tree species	1614:1630	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	7	86	theme	hemipteran	1062:1071	arg1	species					1073:1079	hemipteran species	1062:1079	hemipteran species feeding on the same tree species	1062:1112	Moreover, the proportions of sugars differed significantly between hemipteran species feeding on the same tree species.					
31978201	10	87	theme	sugar	1465:1469	arg1	content					1471:1477	the sugar content	1461:1477	the sugar content	1461:1477	The total contents of amino acids and inorganic ions in the honeydew were much lower than the sugar content.					
31978201	4	88	from	Cinara	658:663	arg1	honeydew					583:590	honeydew	583:590	honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies	583:714	To investigate this issue, honeydew from two Cinara species located on Abies alba as well as from two Cinara and two Physokermes species located on Picea abies were collected.					
31978201	6	89	dep	contained	811:819	arg1	whereas					920:926	whereas	920:926	whereas	920:926	Honeydew of all species contained different proportions of hexoses, sucrose, melezitose, erlose, and further di- and trisaccharides, whereas the phloem exudates of the host trees contained no trisaccharides.					
31978201	11	90	theme	hemipteran	1560:1569	arg1	species					1571:1577	all six hemipteran species	1552:1577	all six hemipteran species	1552:1577	Glutamine and glutamate were predominant amino acids in the honeydew of all six hemipteran species and also in the phloem exudates of both tree species.					
31978201	9	91	theme	different	1346:1354	arg1	species					1362:1368	the different aphid species	1342:1368	the different aphid species	1342:1368	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
31978201	1	92	theme	large	207:211	arg1	honeydew					224:231	honeydew	224:231	honeydew	224:231	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	1	92	theme	large	207:211	arg1	amounts					213:219	large amounts	207:219	large amounts of honeydew that is collected by bees to produce honeydew honey	207:283	Several hemipteran species feed on the phloem sap of plants and produce large amounts of honeydew that is collected by bees to produce honeydew honey.					
31978201	3	93	theme	botanical	469:477	arg1	species					494:500	those botanical and zoological species	463:500	those botanical and zoological species from which the majority of honeydew honey originates	463:553	This is particularly relevant for those botanical and zoological species from which the majority of honeydew honey originates.					
31978201	9	94	theme	species	1362:1368	arg1	honeydew					1330:1337	the honeydew	1326:1337	the honeydew of the different aphid species	1326:1368	The type of the produced oligosaccharides in the aphid-extracts correlated with the oligosaccharide composition in the honeydew of the different aphid species.					
34019973	0	0	theme	effective	96:104	arg1	targeting					106:114	the effective targeting	92:114	the effective targeting of raloxifene to breast tumor	92:144	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	5	1	theme	particle	796:803	arg1	size					805:808	particle size	796:808	particle size less than 100 nm and high entrapment efficiency (77.02%)	796:865	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	7	2	theme	higher	1091:1096	arg1	time					1113:1116	higher mean residence time	1091:1116	higher mean residence time	1091:1116	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	3	3	from	levels	578:583	arg1	factors					561:567	two factors	557:567	two factors at three levels	557:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	9	4	theme	tumor	1446:1450	arg1	rats					1460:1463	the tumor bearing rats	1442:1463	the tumor bearing rats	1442:1463	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	8	5	theme	biodistribution	1182:1196	arg1	study					1198:1202	the biodistribution study	1178:1202	the biodistribution study	1178:1202	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	8	6	theme	other	1283:1287	arg1	tissue					1289:1294	other tissue	1283:1294	other tissue	1283:1294	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	3	7	dep	optimized	612:620	arg1	the					608:610	the	608:610	the	608:610	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	9	8	theme	bearing	1452:1458	arg1	rats					1460:1463	the tumor bearing rats	1442:1463	the tumor bearing rats	1442:1463	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	8	9	theme	enhanced	1215:1222	arg1	concentration					1229:1241	enhanced drug concentration	1215:1241	enhanced drug concentration in tumor	1215:1250	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	1	10	theme	mammary	359:365	arg1	carcinoma					367:375	mammary carcinoma	359:375	mammary carcinoma	359:375	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	7	11	theme	pure	1160:1163	arg1	RA					1165:1166	pure RA	1160:1166	pure RA	1160:1166	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	7	12	theme	In	1029:1030	arg1	study					1053:1057	In vivo pharmacokinetic study	1029:1057	In vivo pharmacokinetic study	1029:1057	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	9	13	theme	rats	1460:1463	arg1	span					1434:1437	life span	1429:1437	life span of the tumor bearing rats	1429:1463	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	6	14	theme	RA	936:937	arg1	profile					925:931	pH-dependent release profile	904:931	pH-dependent release profile of RA	904:937	The developed formulation exhibited pH-dependent release profile of RA when loaded in polymeric micelles and provides substantial compatibility to erythrocytes.					
34019973	9	15	theme	median	1367:1372	arg1	time					1383:1386	the median survival time	1363:1386	the median survival time	1363:1386	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	10	16	theme	overwhelming	1507:1518	arg1	response					1520:1527	the overwhelming response	1503:1527	the overwhelming response of RA	1503:1533	Overall, the results pointed towards the overwhelming response of RA when loaded into micelles made from CP-g-PA.					
34019973	3	17	theme	central	498:504	arg1	methodology					540:550	the central composite design-response surface methodology	494:550	the central composite design-response surface methodology with two factors at three levels	494:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	1	18	theme	palmitic	220:227	arg1	CP-g-PA					235:241	CP-g-PA	235:241	CP-g-PA	235:241	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	18	theme	palmitic	220:227	arg1	acid					229:232	Carbamoylethyl pullulan-grafted palmitic acid	188:232	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA)	188:242	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	18	theme	palmitic	220:227	arg1	polymer					268:274	a novel self-assembled polymer	245:274	a novel self-assembled polymer	245:274	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	19	theme	Carbamoylethyl	188:201	arg1	CP-g-PA					235:241	CP-g-PA	235:241	CP-g-PA	235:241	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	19	theme	Carbamoylethyl	188:201	arg1	acid					229:232	Carbamoylethyl pullulan-grafted palmitic acid	188:232	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA)	188:242	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	19	theme	Carbamoylethyl	188:201	arg1	polymer					268:274	a novel self-assembled polymer	245:274	a novel self-assembled polymer	245:274	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	0	20	theme	raloxifene	119:128	arg1	targeting					106:114	the effective targeting	92:114	the effective targeting of raloxifene to breast tumor	92:144	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	6	21	theme	release	917:923	arg1	profile					925:931	pH-dependent release profile	904:931	pH-dependent release profile of RA	904:937	The developed formulation exhibited pH-dependent release profile of RA when loaded in polymeric micelles and provides substantial compatibility to erythrocytes.					
34019973	5	22	theme	less	810:813	arg1	size					805:808	particle size	796:808	particle size less than 100 nm and high entrapment efficiency (77.02%)	796:865	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	1	23	theme	pullulan-grafted	203:218	arg1	CP-g-PA					235:241	CP-g-PA	235:241	CP-g-PA	235:241	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	23	theme	pullulan-grafted	203:218	arg1	acid					229:232	Carbamoylethyl pullulan-grafted palmitic acid	188:232	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA)	188:242	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	23	theme	pullulan-grafted	203:218	arg1	polymer					268:274	a novel self-assembled polymer	245:274	a novel self-assembled polymer	245:274	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	5	24	theme	high	831:834	arg1	efficiency					847:856	high entrapment efficiency	831:856	high entrapment efficiency (77.02%)	831:865	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	5	24	theme	high	831:834	arg1	%					864:864	77.02%	859:864	77.02%	859:864	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	6	25	theme	pH-dependent	904:915	arg1	profile					925:931	pH-dependent release profile	904:931	pH-dependent release profile of RA	904:937	The developed formulation exhibited pH-dependent release profile of RA when loaded in polymeric micelles and provides substantial compatibility to erythrocytes.					
34019973	7	26	theme	distribution	1132:1143	arg1	volume					1122:1127	volume	1122:1127	volume of distribution	1122:1143	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	7	26	theme	distribution	1132:1143	arg1	time					1113:1116	higher mean residence time	1091:1116	higher mean residence time	1091:1116	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	3	27	used	utilized	589:596	arg2	methodology					540:550	the central composite design-response surface methodology	494:550	the central composite design-response surface methodology with two factors at three levels	494:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	5	28	theme	entrapment	836:845	arg1	efficiency					847:856	high entrapment efficiency	831:856	high entrapment efficiency (77.02%)	831:865	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	5	28	theme	entrapment	836:845	arg1	%					864:864	77.02%	859:864	77.02%	859:864	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	3	29	with	methodology	540:550	arg1	factors					561:567	two factors	557:567	two factors at three levels	557:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	8	30	from	concentration	1229:1241	arg1	tumor					1246:1250	tumor	1246:1250	tumor	1246:1250	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	4	31	theme	in	709:710	arg1	evaluation					717:726	in vitro and in vivo evaluation	696:726	in vitro and in vivo evaluation	696:726	The optimized formulation was subjected to in vitro and in vivo evaluation.					
34019973	2	32	theme	morphological	459:471	arg1	attributes					473:482	various spectral and morphological attributes	438:482	various spectral and morphological attributes	438:482	The synthesized CP-g-PA was confirmed by evaluating through various spectral and morphological attributes.					
34019973	9	33	theme	tumor	1389:1393	arg1	rate					1406:1409	tumor inhibition rate	1389:1409	tumor inhibition rate	1389:1409	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	5	34	theme	polymeric	739:747	arg1	micelles					749:756	RA loaded polymeric micelles	729:756	RA loaded polymeric micelles (RA-PMs)	729:765	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	5	34	theme	polymeric	739:747	arg1	RA-PMs					759:764	RA-PMs	759:764	RA-PMs	759:764	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	4	35	dep	in	696:697	arg1	vitro					699:703	vitro	699:703	vitro	699:703	The optimized formulation was subjected to in vitro and in vivo evaluation.					
34019973	2	36	theme	spectral	446:453	arg1	attributes					473:482	various spectral and morphological attributes	438:482	various spectral and morphological attributes	438:482	The synthesized CP-g-PA was confirmed by evaluating through various spectral and morphological attributes.					
34019973	9	37	theme	inhibition	1395:1404	arg1	rate					1406:1409	tumor inhibition rate	1389:1409	tumor inhibition rate	1389:1409	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	0	38	theme	breast	133:138	arg1	tumor					140:144	breast tumor	133:144	breast tumor	133:144	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	5	39	from	spherical	772:780	arg1	shape					785:789	shape	785:789	shape	785:789	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	2	40	theme	various	438:444	arg1	attributes					473:482	various spectral and morphological attributes	438:482	various spectral and morphological attributes	438:482	The synthesized CP-g-PA was confirmed by evaluating through various spectral and morphological attributes.					
34019973	0	41	theme	pullulan-g-palmitic	44:62	arg1	micelles					79:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	6	42	theme	substantial	986:996	arg1	compatibility					998:1010	substantial compatibility	986:1010	substantial compatibility to erythrocytes	986:1026	The developed formulation exhibited pH-dependent release profile of RA when loaded in polymeric micelles and provides substantial compatibility to erythrocytes.					
34019973	3	43	theme	polymeric	633:641	arg1	micelles					643:650	stable polymeric micelles	626:650	stable polymeric micelles	626:650	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	4	44	theme	in	696:697	arg1	evaluation					717:726	in vitro and in vivo evaluation	696:726	in vitro and in vivo evaluation	696:726	The optimized formulation was subjected to in vitro and in vivo evaluation.					
34019973	0	45	theme	carbamoylethyl	29:42	arg1	micelles					79:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	6	46	theme	developed	872:880	arg1	formulation					882:892	The developed formulation	868:892	The developed formulation	868:892	The developed formulation exhibited pH-dependent release profile of RA when loaded in polymeric micelles and provides substantial compatibility to erythrocytes.					
34019973	8	47	theme	drug	1224:1227	arg1	concentration					1229:1241	enhanced drug concentration	1215:1241	enhanced drug concentration in tumor	1215:1250	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	0	48	theme	polymeric	69:77	arg1	micelles					79:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	9	49	theme	%	1415:1415	arg1	increase					1417:1424	% increase	1415:1424	% increase	1415:1424	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	0	50	theme	preclinical	164:174	arg1	evaluation					176:185	preclinical evaluation	164:185	preclinical evaluation	164:185	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	9	51	theme	survival	1374:1381	arg1	time					1383:1386	the median survival time	1363:1386	the median survival time	1363:1386	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	3	52	theme	surface	532:538	arg1	methodology					540:550	the central composite design-response surface methodology	494:550	the central composite design-response surface methodology with two factors at three levels	494:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	0	53	theme	acid	64:67	arg1	micelles					79:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	carbamoylethyl pullulan-g-palmitic acid polymeric micelles	29:86	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	7	54	theme	residence	1103:1111	arg1	time					1113:1116	higher mean residence time	1091:1116	higher mean residence time	1091:1116	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	5	55	with	spherical	772:780	arg1	size					805:808	particle size	796:808	particle size less than 100 nm and high entrapment efficiency (77.02%)	796:865	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	3	56	theme	stable	626:631	arg1	micelles					643:650	stable polymeric micelles	626:650	stable polymeric micelles	626:650	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	8	57	from	concentration	1266:1278	arg1	tissue					1289:1294	other tissue	1283:1294	other tissue	1283:1294	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	9	58	with	treatment	1326:1334	arg1	RA-PMs					1341:1346	RA-PMs	1341:1346	RA-PMs	1341:1346	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	6	59	theme	polymeric	954:962	arg1	micelles					964:971	polymeric micelles	954:971	polymeric micelles	954:971	The developed formulation exhibited pH-dependent release profile of RA when loaded in polymeric micelles and provides substantial compatibility to erythrocytes.					
34019973	5	60	theme	RA	729:730	arg1	micelles					749:756	RA loaded polymeric micelles	729:756	RA loaded polymeric micelles (RA-PMs)	729:765	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	5	60	theme	RA	729:730	arg1	RA-PMs					759:764	RA-PMs	759:764	RA-PMs	759:764	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	8	61	theme	pure	1311:1314	arg1	drug					1316:1319	pure drug	1311:1319	pure drug	1311:1319	Besides, the biodistribution study manifested enhanced drug concentration in tumor and decreased concentration in other tissue as compared to pure drug.					
34019973	3	62	theme	composite	506:514	arg1	methodology					540:550	the central composite design-response surface methodology	494:550	the central composite design-response surface methodology with two factors at three levels	494:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	7	63	dep	In	1029:1030	arg1	vivo					1032:1035	vivo	1032:1035	vivo	1032:1035	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	5	64	theme	loaded	732:737	arg1	micelles					749:756	RA loaded polymeric micelles	729:756	RA loaded polymeric micelles (RA-PMs)	729:765	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	5	64	theme	loaded	732:737	arg1	RA-PMs					759:764	RA-PMs	759:764	RA-PMs	759:764	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	5	65	from	shape	785:789	arg1	spherical					772:780	spherical	772:780	spherical	772:780	RA loaded polymeric micelles (RA-PMs) were spherical in shape with particle size less than 100 nm and high entrapment efficiency (77.02%).					
34019973	4	66	dep	in	709:710	arg1	vivo					712:715	vivo	712:715	vivo	712:715	The optimized formulation was subjected to in vitro and in vivo evaluation.					
34019973	3	67	theme	design-response	516:530	arg1	methodology					540:550	the central composite design-response surface methodology	494:550	the central composite design-response surface methodology with two factors at three levels	494:583	Further, the central composite design-response surface methodology with two factors at three levels was utilized to obtain the optimized and stable polymeric micelles.					
34019973	1	68	theme	novel	247:251	arg1	polymer					268:274	a novel self-assembled polymer	245:274	a novel self-assembled polymer	245:274	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	68	theme	novel	247:251	arg1	acid					229:232	Carbamoylethyl pullulan-grafted palmitic acid	188:232	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA)	188:242	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	10	69	theme	RA	1532:1533	arg1	response					1520:1527	the overwhelming response	1503:1527	the overwhelming response of RA	1503:1533	Overall, the results pointed towards the overwhelming response of RA when loaded into micelles made from CP-g-PA.					
34019973	7	70	theme	mean	1098:1101	arg1	time					1113:1116	higher mean residence time	1091:1116	higher mean residence time	1091:1116	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	7	71	theme	pharmacokinetic	1037:1051	arg1	study					1053:1057	In vivo pharmacokinetic study	1029:1057	In vivo pharmacokinetic study	1029:1057	In vivo pharmacokinetic study demonstrates that RA-PMs offers higher mean residence time and volume of distribution as compared to pure RA.					
34019973	2	72	theme	synthesized	382:392	arg1	CP-g-PA					394:400	The synthesized CP-g-PA	378:400	The synthesized CP-g-PA	378:400	The synthesized CP-g-PA was confirmed by evaluating through various spectral and morphological attributes.					
34019973	1	73	theme	self-assembled	253:266	arg1	polymer					268:274	a novel self-assembled polymer	245:274	a novel self-assembled polymer	245:274	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	1	73	theme	self-assembled	253:266	arg1	acid					229:232	Carbamoylethyl pullulan-grafted palmitic acid	188:232	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA)	188:242	Carbamoylethyl pullulan-grafted palmitic acid (CP-g-PA), a novel self-assembled polymer was synthesized and examined for its efficacy in delivering the raloxifene (RA) to mammary carcinoma.					
34019973	0	74	theme	micelles	79:86	arg1	feasibility					14:24	the feasibility	10:24	the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor	10:144	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	0	75	dep	Exploring	0:8	arg1	Optimization					147:158	Optimization	147:158	Optimization	147:158	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	0	75	dep	Exploring	0:8	arg1	evaluation					176:185	preclinical evaluation	164:185	preclinical evaluation	164:185	Exploring the feasibility of carbamoylethyl pullulan-g-palmitic acid polymeric micelles for the effective targeting of raloxifene to breast tumor: Optimization and preclinical evaluation.					
34019973	9	76	theme	life	1429:1432	arg1	span					1434:1437	life span	1429:1437	life span of the tumor bearing rats	1429:1463	The treatment with RA-PMs also increases the median survival time, tumor inhibition rate and % increase in life span of the tumor bearing rats.					
34019973	4	77	theme	optimized	657:665	arg1	formulation					667:677	The optimized formulation	653:677	The optimized formulation	653:677	The optimized formulation was subjected to in vitro and in vivo evaluation.					
32376698	4	0	theme	fecal	617:621	arg1	studies					636:642	in vitro fecal fermentation studies	608:642	in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW])	608:782	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	2	1	theme	fiber	373:377	arg1	fermentation					379:390	soluble fiber fermentation	365:390	soluble fiber fermentation	365:390	However, it has not been elucidated whether subtle structural variations might impact soluble fiber fermentation by colonic microbiota.					
32376698	10	2	theme	structures	2254:2263	arg1	vastness					2236:2243	the vastness	2232:2243	the vastness of fiber structures in terms of their microbial utilization	2232:2303	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	3	3	theme	microbiota	583:592	arg1	structure					538:546	community structure	528:546	community structure	528:546	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	3	3	theme	microbiota	583:592	arg1	output					562:567	metabolic output	552:567	metabolic output	552:567	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	8	4	theme	health	1759:1764	arg1	outcomes					1766:1773	health outcomes	1759:1773	health outcomes	1759:1773	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	12	5	theme	different	2580:2588	arg1	classes					2590:2596	different classes	2580:2596	different classes of wheat	2580:2605	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	4	6	theme	wheat	696:700	arg1	classes					685:691	different classes	675:691	different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW	675:749	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	13	7	theme	structural	2786:2795	arg1	resolution					2797:2806	much higher structural resolution	2774:2806	much higher structural resolution	2774:2806	This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.					
32376698	7	8	theme	dietary	1492:1498	arg1	fiber					1500:1504	a dietary fiber	1490:1504	a dietary fiber	1490:1504	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	7	9	theme	subtle	1455:1460	arg1	changes					1462:1468	subtle changes	1455:1468	subtle changes in the structure of a dietary fiber	1455:1504	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	4	10	theme	hard	703:706	arg1	[AXHRS					719:724	hard red spring [AXHRS	703:724	hard red spring [AXHRS	703:724	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	10	theme	hard	703:706	arg1	wheat					696:700	wheat	696:700	wheat (hard red spring [AXHRS]	696:725	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	2	11	theme	subtle	323:328	arg1	variations					341:350	subtle structural variations	323:350	subtle structural variations	323:350	However, it has not been elucidated whether subtle structural variations might impact soluble fiber fermentation by colonic microbiota.					
32376698	4	12	from	classes	685:691	arg1	arabinoxylans					650:662	arabinoxylans	650:662	arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW]	650:750	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	12	from	classes	685:691	arg1	AXs					665:667	AXs	665:667	AXs	665:667	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	6	13	theme	Amplicon	982:989	arg1	sequencing					991:1000	Amplicon sequencing	982:1000	Amplicon sequencing	982:1000	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	12	14	theme	bran	2547:2550	arg1	arabinoxylans					2552:2564	bran arabinoxylans	2547:2564	bran arabinoxylans obtained from different classes of wheat	2547:2605	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	10	15	theme	detailed	2314:2321	arg1	analysis					2347:2354	detailed carbohydrate structural analysis	2314:2354	detailed carbohydrate structural analysis	2314:2354	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	8	16	theme	factors	1970:1976	arg1	factors					1970:1976	the most important factors	1951:1976	the most important factors shaping the colonic microbiota composition	1951:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	8	16	theme	factors	1970:1976	arg1	one					1944:1946	one	1944:1946	one	1944:1946	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	8	16	theme	factors	1970:1976	arg1	Diet					1858:1861	employed.IMPORTANCE Diet	1838:1861	employed.IMPORTANCE Diet	1838:1861	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	6	17	theme	evenness	1122:1129	arg1	structure					1075:1083	a distinct community structure	1054:1083	a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures	1054:1171	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	1	18	theme	due	215:217	arg1	source					208:213	source	208:213	source due to numerous possible linkage configurations among monomers	208:276	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	10	19	theme	studies	2208:2214	arg1	majority					2190:2197	the majority	2186:2197	the majority of these studies	2186:2214	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	1	20	theme	numerous	222:229	arg1	configurations					248:261	numerous possible linkage configurations	222:261	numerous possible linkage configurations among monomers	222:276	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	9	21	theme	many	2035:2038	arg1	studies					2040:2046	many studies	2035:2046	many studies	2035:2046	Accordingly, many studies have been conducted to explore dietary fiber types that could predictably manipulate the colonic microbiota for improved health.					
32376698	4	22	theme	hard	728:731	arg1	wheat					696:700	wheat	696:700	wheat (hard red spring [AXHRS]	696:725	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	22	theme	hard	728:731	arg1	[AXHRW					744:749	hard red winter [AXHRW	728:749	hard red winter [AXHRW	728:749	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	8	23	theme	microbiota	1998:2007	arg1	composition					2009:2019	the colonic microbiota composition	1986:2019	the colonic microbiota composition	1986:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	13	24	theme	gut	2869:2871	arg1	microbiota					2873:2882	gut microbiota	2869:2882	gut microbiota	2869:2882	This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.					
32376698	8	25	theme	employed.IMPORTANCE	1838:1856	arg1	factors					1970:1976	the most important factors	1951:1976	the most important factors shaping the colonic microbiota composition	1951:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	8	25	theme	employed.IMPORTANCE	1838:1856	arg1	one					1944:1946	one	1944:1946	one	1944:1946	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	8	25	theme	employed.IMPORTANCE	1838:1856	arg1	Diet					1858:1861	employed.IMPORTANCE Diet	1838:1861	employed.IMPORTANCE Diet	1838:1861	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	4	26	theme	winter	737:742	arg1	wheat					696:700	wheat	696:700	wheat (hard red spring [AXHRS]	696:725	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	26	theme	winter	737:742	arg1	[AXHRW					744:749	hard red winter [AXHRW	728:749	hard red winter [AXHRW	728:749	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	12	27	theme	divergent	2689:2697	arg1	communities					2709:2719	divergent microbial communities	2689:2719	divergent microbial communities	2689:2719	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	4	28	theme	spring	712:717	arg1	[AXHRS					719:724	hard red spring [AXHRS	703:724	hard red spring [AXHRS	703:724	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	28	theme	spring	712:717	arg1	wheat					696:700	wheat	696:700	wheat (hard red spring [AXHRS]	696:725	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	5	29	theme	shorter	898:904	arg1	backbone					906:913	a significantly shorter backbone	882:913	a significantly shorter backbone	882:913	Carbohydrate analyses revealed that AXSRW was characterized by a significantly shorter backbone and increased branching compared with those of the hard varieties.					
32376698	7	30	theme	fibers	1640:1645	arg1	functions					1619:1627	physiological functions	1605:1627	physiological functions of dietary fibers	1605:1645	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	12	31	theme	metabolic	2725:2733	arg1	outputs					2735:2741	metabolic outputs	2725:2741	metabolic outputs	2725:2741	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	7	32	theme	metabolic	1269:1277	arg1	output					1279:1284	metabolic output	1269:1284	metabolic output	1269:1284	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	1	33	theme	carbohydrates	174:186	arg1	structures					146:155	The chemical structures	133:155	The chemical structures of soluble fiber carbohydrates	133:186	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	9	34	theme	fiber	2087:2091	arg1	types					2093:2097	dietary fiber types	2079:2097	dietary fiber types that could predictably manipulate the colonic microbiota for improved health	2079:2174	Accordingly, many studies have been conducted to explore dietary fiber types that could predictably manipulate the colonic microbiota for improved health.					
32376698	4	35	theme	red	764:766	arg1	[AXSRW					775:780	spring red winter [AXSRW	757:780	spring red winter [AXSRW	757:780	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	12	36	theme	microbial	2699:2707	arg1	communities					2709:2719	divergent microbial communities	2689:2719	divergent microbial communities	2689:2719	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	4	37	dep	in	608:609	arg1	vitro					611:615	vitro	611:615	vitro	611:615	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	7	38	theme	physiological	1605:1617	arg1	functions					1619:1627	physiological functions	1605:1627	physiological functions of dietary fibers	1605:1645	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	7	39	dep	favored	1180:1186	arg1	whereas					1213:1219	whereas	1213:1219	whereas	1213:1219	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	10	40	theme	utilization	2293:2303	arg1	terms					2268:2272	terms	2268:2272	terms of their microbial utilization	2268:2303	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	0	41	theme	Gut	117:119	arg1	Microbiota					121:130	Gut Microbiota	117:130	Gut Microbiota	117:130	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	8	42	theme	fibers	1914:1919	arg1	consumption					1891:1901	consumption	1891:1901	consumption of dietary fibers	1891:1919	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	6	43	theme	richness	1109:1116	arg1	structure					1075:1083	a distinct community structure	1054:1083	a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures	1054:1171	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	0	44	theme	Subtle	0:5	arg1	Variations					7:16	Subtle Variations	0:16	Subtle Variations in Dietary-Fiber Fine Structure	0:48	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	3	45	dep	structure	538:546	arg1	the					524:526	the	524:526	the	524:526	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	12	46	theme	fecal	2514:2518	arg1	studies					2533:2539	in vitro fecal fermentation studies	2505:2539	in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat	2505:2605	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	10	47	theme	carbohydrate	2323:2334	arg1	analysis					2347:2354	detailed carbohydrate structural analysis	2314:2354	detailed carbohydrate structural analysis	2314:2354	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	12	48	theme	fiber	2673:2677	arg1	structure					2650:2658	the structure	2646:2658	the structure of a dietary fiber	2646:2677	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	5	49	theme	Carbohydrate	819:830	arg1	analyses					832:839	Carbohydrate analyses	819:839	Carbohydrate analyses	819:839	Carbohydrate analyses revealed that AXSRW was characterized by a significantly shorter backbone and increased branching compared with those of the hard varieties.					
32376698	5	50	theme	hard	966:969	arg1	varieties					971:979	the hard varieties	962:979	the hard varieties	962:979	Carbohydrate analyses revealed that AXSRW was characterized by a significantly shorter backbone and increased branching compared with those of the hard varieties.					
32376698	3	51	theme	structural	467:476	arg1	variations					478:487	subtle structural variations	460:487	subtle structural variations in a soluble polysaccharide	460:515	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	0	52	theme	Fine	35:38	arg1	Structure					40:48	Dietary-Fiber Fine Structure	21:48	Dietary-Fiber Fine Structure	21:48	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	7	53	theme	microbiota	1569:1578	arg1	function					1549:1556	function	1549:1556	function	1549:1556	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	7	53	theme	microbiota	1569:1578	arg1	composition					1533:1543	the composition	1529:1543	the composition	1529:1543	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	12	54	from	changes	2635:2641	arg1	structure					2650:2658	the structure	2646:2658	the structure of a dietary fiber	2646:2677	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	6	55	theme	distinct	1056:1063	arg1	structure					1075:1083	a distinct community structure	1054:1083	a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures	1054:1171	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	9	56	theme	improved	2160:2167	arg1	health					2169:2174	improved health	2160:2174	improved health	2160:2174	Accordingly, many studies have been conducted to explore dietary fiber types that could predictably manipulate the colonic microbiota for improved health.					
32376698	3	57	from	variations	478:487	arg1	polysaccharide					502:515	a soluble polysaccharide	492:515	a soluble polysaccharide	492:515	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	2	58	theme	soluble	365:371	arg1	fermentation					379:390	soluble fiber fermentation	365:390	soluble fiber fermentation	365:390	However, it has not been elucidated whether subtle structural variations might impact soluble fiber fermentation by colonic microbiota.					
32376698	7	59	theme	propionate	1333:1342	arg1	production					1344:1353	higher propionate production	1326:1353	higher propionate production	1326:1353	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	12	60	theme	subtle	2628:2633	arg1	changes					2635:2641	even subtle changes	2623:2641	even subtle changes in the structure of a dietary fiber	2623:2677	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	12	61	theme	in	2505:2506	arg1	studies					2533:2539	in vitro fecal fermentation studies	2505:2539	in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat	2505:2605	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	7	62	dep	Prevotella	1245:1254	arg1	varied					1286:1291	varied	1286:1291	varied between hard and soft varieties	1286:1323	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	3	63	theme	soluble	494:500	arg1	polysaccharide					502:515	a soluble polysaccharide	492:515	a soluble polysaccharide	492:515	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	13	64	theme	dietary	2849:2855	arg1	fibers					2857:2862	dietary fibers	2849:2862	dietary fibers	2849:2862	This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.					
32376698	1	65	theme	possible	231:238	arg1	configurations					248:261	numerous possible linkage configurations	222:261	numerous possible linkage configurations among monomers	222:276	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	6	66	theme	hard-AX-fermenting	1145:1162	arg1	cultures					1164:1171	hard-AX-fermenting cultures	1145:1171	hard-AX-fermenting cultures	1145:1171	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	4	67	theme	identical	789:797	arg1	microbiota					807:816	identical initial microbiota	789:816	identical initial microbiota	789:816	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	2	68	theme	colonic	395:401	arg1	microbiota					403:412	colonic microbiota	395:412	colonic microbiota	395:412	However, it has not been elucidated whether subtle structural variations might impact soluble fiber fermentation by colonic microbiota.					
32376698	8	69	theme	dietary	1724:1730	arg1	fiber					1732:1736	dietary fiber	1724:1736	dietary fiber	1724:1736	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	6	70	theme	AXSRW	1036:1040	arg1	fermentation					1020:1031	fermentation	1020:1031	fermentation of AXSRW	1020:1040	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	7	71	theme	soft	1310:1313	arg1	varieties					1315:1323	hard and soft varieties	1301:1323	hard and soft varieties	1301:1323	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	13	72	with	interactions	2833:2844	arg1	microbiota					2873:2882	gut microbiota	2869:2882	gut microbiota	2869:2882	This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.					
32376698	3	73	theme	community	528:536	arg1	structure					538:546	community structure	528:546	community structure	528:546	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	7	74	theme	hard	1301:1304	arg1	varieties					1315:1323	hard and soft varieties	1301:1323	hard and soft varieties	1301:1323	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	3	75	theme	fermenting	572:581	arg1	microbiota					583:592	fermenting microbiota	572:592	fermenting microbiota	572:592	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	7	76	theme	fiber	1500:1504	arg1	structure					1477:1485	the structure	1473:1485	the structure of a dietary fiber	1473:1504	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	4	77	theme	fermentation	623:634	arg1	studies					636:642	in vitro fecal fermentation studies	608:642	in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW])	608:782	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	13	78	theme	higher	2779:2784	arg1	resolution					2797:2806	much higher structural resolution	2774:2806	much higher structural resolution	2774:2806	This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.					
32376698	12	79	theme	wheat	2601:2605	arg1	classes					2590:2596	different classes	2580:2596	different classes of wheat	2580:2605	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	8	80	theme	important	1960:1968	arg1	factors					1970:1976	the most important factors	1951:1976	the most important factors shaping the colonic microbiota composition	1951:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	2	81	theme	structural	330:339	arg1	variations					341:350	subtle structural variations	323:350	subtle structural variations	323:350	However, it has not been elucidated whether subtle structural variations might impact soluble fiber fermentation by colonic microbiota.					
32376698	11	82	theme	conflicting	2384:2394	arg1	results					2396:2402	conflicting results	2384:2402	conflicting results	2384:2402	In some cases, this causes conflicting results to arise between studies using (theoretically) the same fibers.					
32376698	9	83	theme	dietary	2079:2085	arg1	types					2093:2097	dietary fiber types	2079:2097	dietary fiber types that could predictably manipulate the colonic microbiota for improved health	2079:2174	Accordingly, many studies have been conducted to explore dietary fiber types that could predictably manipulate the colonic microbiota for improved health.					
32376698	8	84	theme	colonic	1990:1996	arg1	composition					2009:2019	the colonic microbiota composition	1986:2019	the colonic microbiota composition	1986:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	4	85	theme	different	675:683	arg1	classes					685:691	different classes	675:691	different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW	675:749	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	12	86	theme	dietary	2665:2671	arg1	fiber					2673:2677	a dietary fiber	2663:2677	a dietary fiber	2663:2677	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	10	87	theme	fiber	2248:2252	arg1	structures					2254:2263	fiber structures	2248:2263	fiber structures	2248:2263	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	8	88	theme	carbohydrates	1824:1836	arg1	structures					1806:1815	the structures	1802:1815	the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition	1802:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	1	89	theme	chemical	137:144	arg1	structures					146:155	The chemical structures	133:155	The chemical structures of soluble fiber carbohydrates	133:186	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	10	90	theme	microbial	2283:2291	arg1	utilization					2293:2303	their microbial utilization	2277:2303	their microbial utilization	2277:2303	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	4	91	theme	red	733:735	arg1	wheat					696:700	wheat	696:700	wheat (hard red spring [AXHRS]	696:725	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	91	theme	red	733:735	arg1	[AXHRW					744:749	hard red winter [AXHRW	728:749	hard red winter [AXHRW	728:749	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	7	92	from	changes	1462:1468	arg1	structure					1477:1485	the structure	1473:1485	the structure of a dietary fiber	1473:1504	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	7	93	theme	dietary	1632:1638	arg1	fibers					1640:1645	dietary fibers	1632:1645	dietary fibers	1632:1645	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	4	94	theme	red	708:710	arg1	[AXHRS					719:724	hard red spring [AXHRS	703:724	hard red spring [AXHRS	703:724	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	4	94	theme	red	708:710	arg1	wheat					696:700	wheat	696:700	wheat (hard red spring [AXHRS]	696:725	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	0	95	theme	Metabolic	95:103	arg1	Function					105:112	Metabolic Function	95:112	Metabolic Function	95:112	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	12	96	dep	in	2505:2506	arg1	vitro					2508:2512	vitro	2508:2512	vitro	2508:2512	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	1	97	theme	fiber	168:172	arg1	carbohydrates					174:186	soluble fiber carbohydrates	160:186	soluble fiber carbohydrates	160:186	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	0	98	from	Variations	7:16	arg1	Structure					40:48	Dietary-Fiber Fine Structure	21:48	Dietary-Fiber Fine Structure	21:48	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	8	99	dep	structures	1806:1815	arg1	recognized					1930:1939	recognized	1930:1939	is well recognized as one of the most important factors shaping the colonic microbiota composition	1922:2019	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	4	100	theme	spring	757:762	arg1	[AXSRW					775:780	spring red winter [AXSRW	757:780	spring red winter [AXSRW	757:780	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	0	101	theme	Microbiota	121:130	arg1	Composition					79:89	Composition	79:89	Composition	79:89	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	0	101	theme	Microbiota	121:130	arg1	Function					105:112	Metabolic Function	95:112	Metabolic Function	95:112	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	7	102	theme	higher	1383:1388	arg1	butyrate					1390:1397	higher butyrate	1383:1397	higher butyrate	1383:1397	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	4	103	theme	winter	768:773	arg1	[AXSRW					775:780	spring red winter [AXSRW	757:780	spring red winter [AXSRW	757:780	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	3	104	theme	subtle	460:465	arg1	variations					478:487	subtle structural variations	460:487	subtle structural variations in a soluble polysaccharide	460:515	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	8	105	theme	dietary	1906:1912	arg1	fibers					1914:1919	dietary fibers	1906:1919	dietary fibers	1906:1919	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	6	106	theme	higher	1102:1107	arg1	richness					1109:1116	richness	1109:1116	richness	1109:1116	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	12	107	theme	fermentation	2520:2531	arg1	studies					2533:2539	in vitro fecal fermentation studies	2505:2539	in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat	2505:2605	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	10	108	theme	structural	2336:2345	arg1	analysis					2347:2354	detailed carbohydrate structural analysis	2314:2354	detailed carbohydrate structural analysis	2314:2354	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	0	109	theme	Dietary-Fiber	21:33	arg1	Structure					40:48	Dietary-Fiber Fine Structure	21:48	Dietary-Fiber Fine Structure	21:48	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	10	110	from	vastness	2236:2243	arg1	terms					2268:2272	terms	2268:2272	terms of their microbial utilization	2268:2303	However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis.					
32376698	11	111	theme	same	2455:2458	arg1	fibers					2460:2465	the same fibers	2451:2465	(theoretically) the same fibers	2435:2465	In some cases, this causes conflicting results to arise between studies using (theoretically) the same fibers.					
32376698	11	111	theme	same	2455:2458	arg1	theoretically					2436:2448	theoretically	2436:2448	theoretically	2436:2448	In some cases, this causes conflicting results to arise between studies using (theoretically) the same fibers.					
32376698	0	112	dep	Composition	79:89	arg1	the					75:77	the	75:77	the	75:77	Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota.					
32376698	4	113	theme	in	608:609	arg1	studies					636:642	in vitro fecal fermentation studies	608:642	in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW])	608:782	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	9	114	theme	colonic	2137:2143	arg1	microbiota					2145:2154	the colonic microbiota	2133:2154	the colonic microbiota for improved health	2133:2174	Accordingly, many studies have been conducted to explore dietary fiber types that could predictably manipulate the colonic microbiota for improved health.					
32376698	6	115	theme	community	1065:1073	arg1	structure					1075:1083	a distinct community structure	1054:1083	a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures	1054:1171	Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures.					
32376698	4	116	theme	initial	799:805	arg1	microbiota					807:816	identical initial microbiota	789:816	identical initial microbiota	789:816	We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota.					
32376698	7	117	theme	higher	1326:1331	arg1	production					1344:1353	higher propionate production	1326:1353	higher propionate production	1326:1353	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	8	118	dep	factors	1970:1976	arg1	shaping					1978:1984	shaping	1978:1984	shaping	1978:1984	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
32376698	1	119	theme	soluble	160:166	arg1	carbohydrates					174:186	soluble fiber carbohydrates	160:186	soluble fiber carbohydrates	160:186	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	7	120	theme	colonic	1561:1567	arg1	microbiota					1569:1578	colonic microbiota	1561:1578	colonic microbiota	1561:1578	AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent.					
32376698	12	121	theme	even	2623:2626	arg1	changes					2635:2641	even subtle changes	2623:2641	even subtle changes in the structure of a dietary fiber	2623:2677	In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs.					
32376698	13	122	theme	fibers	2857:2862	arg1	interactions					2833:2844	interactions	2833:2844	interactions of dietary fibers with gut microbiota	2833:2882	This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.					
32376698	1	123	theme	linkage	240:246	arg1	configurations					248:261	numerous possible linkage configurations	222:261	numerous possible linkage configurations among monomers	222:276	The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers.					
32376698	3	124	theme	metabolic	552:560	arg1	output					562:567	metabolic output	552:567	metabolic output	552:567	In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota.					
32376698	8	125	theme	gut	1739:1741	arg1	microbiota					1743:1752	gut microbiota	1739:1752	gut microbiota	1739:1752	Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed.IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition.					
31928136	5	0	theme	different	1190:1198	arg1	groups					1200:1205	different groups	1190:1205	different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric	1190:1312	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	8	1	theme	Scanning	1657:1664	arg1	SEM					1687:1689	SEM	1687:1689	SEM	1687:1689	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	8	1	theme	Scanning	1657:1664	arg1	microscopy					1675:1684	Scanning electron microscopy	1657:1684	Scanning electron microscopy (SEM) observations	1657:1703	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	9	2	theme	inverted	1897:1904	arg1	observations					1917:1928	inverted microscope observations	1897:1928	inverted microscope observations	1897:1928	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	8	3	theme	culture	1874:1880	arg1	3 days					1864:1869	3 days	1864:1869	3 days of culture	1864:1880	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	3	4	theme	bi-component	743:754	arg1	membrane					756:763	The bi-component membrane	739:763	The bi-component membrane	739:763	The bi-component membrane was crosslinked by glutaraldehyde vapor to maintain its fiber morphology in the wet stage.					
31928136	1	5	theme	PVA	209:211	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	5	theme	PVA	209:211	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	5	6	theme	different	1064:1072	arg1	PCL/Gel					1109:1115	PCL/Gel	1109:1115	PCL/Gel	1109:1115	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	6	theme	different	1064:1072	arg1	PVA					1091:1093	PVA	1091:1093	PVA	1091:1093	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	6	theme	different	1064:1072	arg1	PVA/CS					1096:1101	PVA/CS	1096:1101	PVA/CS	1096:1101	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	6	theme	different	1064:1072	arg1	PVA/CS-PCL/Gel					1121:1134	PVA/CS-PCL/Gel	1121:1134	PVA/CS-PCL/Gel	1121:1134	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	6	theme	different	1064:1072	arg1	scaffolds					1080:1088	five different fiber scaffolds	1059:1088	five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel)	1059:1135	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	6	theme	different	1064:1072	arg1	PCL					1104:1106	PCL	1104:1106	PCL	1104:1106	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	1	7	theme	/chitosan	213:221	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	7	theme	/chitosan	213:221	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	7	8	theme	cells	1650:1654	arg1	adhesion					1619:1626	adhesion	1619:1626	adhesion	1619:1626	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	7	8	theme	cells	1650:1654	arg1	proliferation					1633:1645	proliferation	1633:1645	proliferation	1633:1645	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	7	8	theme	cells	1650:1654	arg1	growth					1611:1616	growth	1611:1616	growth	1611:1616	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	2	9	theme	composite	513:521	arg1	nanofibers					523:532	PVA/CS composite nanofibers	506:532	PVA/CS composite nanofibers (102 ± 52 nm)	506:546	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	2	9	theme	composite	513:521	arg1	102 ± 52 nm					535:545	102 ± 52 nm	535:545	102 ± 52 nm	535:545	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	10	10	theme	extracellular	2155:2167	arg1	matrix					2169:2174	the native extracellular matrix	2144:2174	the native extracellular matrix	2144:2174	Our results provide the underlying insights needed to guide the design of the native extracellular matrix.					
31928136	1	11	theme	CS	223:224	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	11	theme	CS	223:224	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	2	12	theme	PCL/Gelcomposite	552:567	arg1	2.5 ± 1.0 µm					581:592	2.5 ± 1.0 µm	581:592	2.5 ± 1.0 µm	581:592	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	2	12	theme	PCL/Gelcomposite	552:567	arg1	microfiber					569:578	PCL/Gelcomposite microfiber	552:578	PCL/Gelcomposite microfiber (2.5 ± 1.0 µm)	552:593	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	9	13	theme	complex	1966:1972	arg1	membrane					1982:1989	the PVA/CS-PCL/Gel complex fibrous membrane	1947:1989	the PVA/CS-PCL/Gel complex fibrous membrane	1947:1989	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	6	14	theme	Cell	1315:1318	arg1	results					1328:1334	Cell culture results	1315:1334	Cell culture results	1315:1334	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	4	15	theme	membranes	915:923	arg1	shrinkage					868:876	shrinkage	868:876	shrinkage	868:876	Morphology, shrinkage and spectroscopic of the electrospun membranes were characterized.					
31928136	4	15	theme	membranes	915:923	arg1	Morphology					856:865	Morphology	856:865	Morphology	856:865	Morphology, shrinkage and spectroscopic of the electrospun membranes were characterized.					
31928136	4	15	theme	membranes	915:923	arg1	spectroscopic					882:894	spectroscopic	882:894	spectroscopic	882:894	Morphology, shrinkage and spectroscopic of the electrospun membranes were characterized.					
31928136	2	16	theme	rotating	673:680	arg1	mandrel					682:688	a rotating mandrel	671:688	a rotating mandrel to generate a bi-component multi-scale membrane	671:736	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	3	17	theme	wet	845:847	arg1	stage					849:853	the wet stage	841:853	the wet stage	841:853	The bi-component membrane was crosslinked by glutaraldehyde vapor to maintain its fiber morphology in the wet stage.					
31928136	2	18	theme	cells	479:483	arg1	migration					466:474	the migration	462:474	the migration of cells inside the scaffold	462:503	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	2	19	theme	multi-scale	717:727	arg1	membrane					729:736	a bi-component multi-scale membrane	702:736	a bi-component multi-scale membrane	702:736	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	5	20	theme	MTT	1297:1299	arg1	colorimetric					1301:1312	MTT colorimetric	1297:1312	MTT colorimetric	1297:1312	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	1	21	theme	bi-component	177:188	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	21	theme	bi-component	177:188	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	0	22	theme	extracellular	96:108	arg1	matrix					110:115	simulating extracellular matrix	85:115	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.	0:169	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	2	23	theme	opposite	640:647	arg1	syringes					649:656	the two opposite syringes	632:656	the two opposite syringes	632:656	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	5	24	theme	microscope	1263:1272	arg1	assay					1287:1291	inverted microscope observations assay	1254:1291	inverted microscope observations assay	1254:1291	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	3	25	theme	fiber	821:825	arg1	morphology					827:836	its fiber morphology	817:836	its fiber morphology	817:836	The bi-component membrane was crosslinked by glutaraldehyde vapor to maintain its fiber morphology in the wet stage.					
31928136	6	26	theme	chitosan	1370:1377	arg1	incorporation					1353:1365	the incorporation	1349:1365	the incorporation of chitosan and gelatin	1349:1389	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	7	27	theme	better	1576:1581	arg1	environment					1583:1593	a better environment	1574:1593	a better environment to increase the growth, adhesion, and proliferation of cells	1574:1654	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	9	28	theme	systems	2051:2057	arg1	scaffolds					2059:2067	other single component/scale systems scaffolds	2022:2067	other single component/scale systems scaffolds	2022:2067	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	1	29	with	comparison	328:337	arg1	systems					371:377	several other single scale systems	344:377	several other single scale systems	344:377	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	0	30	theme	cellular	130:137	arg1	infiltration					139:150	enhanced cellular infiltration	121:150	enhanced cellular infiltration	121:150	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	1	31	theme	several	344:350	arg1	systems					371:377	several other single scale systems	344:377	several other single scale systems	344:377	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	0	32	theme	simulating	85:94	arg1	matrix					110:115	simulating extracellular matrix	85:115	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.	0:169	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	5	33	theme	cell	1150:1153	arg1	adhesion					1155:1162	cell adhesion	1150:1162	cell adhesion	1150:1162	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	2	34	theme	native	393:398	arg1	matrix					414:419	the native extracellular matrix	389:419	the native extracellular matrix in composition and structure	389:448	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	9	35	theme	better	2001:2006	arg1	activity					2008:2015	better activity	2001:2015	better activity	2001:2015	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	5	36	theme	developed	963:971	arg1	membrane					984:991	the newly developed multiscale membrane	953:991	the newly developed multiscale membrane	953:991	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	6	37	theme	cell	1423:1426	arg1	spreading					1428:1436	cell spreading	1423:1436	cell spreading	1423:1436	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	1	38	theme	multiscale	266:275	arg1	scaffold					289:296	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold	171:296	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold	171:296	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	0	39	theme	PVA/CS-PCL/gel	36:49	arg1	scaffold					75:82	PVA/CS-PCL/gel multi-scale electrospun scaffold	36:82	PVA/CS-PCL/gel multi-scale electrospun scaffold	36:82	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	7	40	theme	PVA/CS-PCL/Gel	1532:1545	arg1	scaffolds					1556:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds	1517:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds	1517:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	0	41	theme	electrospun	63:73	arg1	scaffold					75:82	PVA/CS-PCL/gel multi-scale electrospun scaffold	36:82	PVA/CS-PCL/gel multi-scale electrospun scaffold	36:82	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	8	42	theme	electron	1666:1673	arg1	SEM					1687:1689	SEM	1687:1689	SEM	1687:1689	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	8	42	theme	electron	1666:1673	arg1	microscopy					1675:1684	Scanning electron microscopy	1657:1684	Scanning electron microscopy (SEM) observations	1657:1703	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	5	43	theme	mesenchymal	1004:1014	arg1	MSCs					1028:1031	MSCs	1028:1031	MSCs	1028:1031	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	43	theme	mesenchymal	1004:1014	arg1	cells					1021:1025	mesenchymal stem cells	1004:1025	mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel)	1004:1135	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	6	44	theme	scaffolds	1491:1499	arg1	performance					1459:1469	the performance	1455:1469	the performance of single component scaffolds of PVA and PCL	1455:1514	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	5	45	theme	scanning	1224:1231	arg1	microscopy					1242:1251	scanning electron microscopy	1224:1251	scanning electron microscopy	1224:1251	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	0	46	dep	Fabrication	0:10	arg1	matrix					110:115	simulating extracellular matrix	85:115	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.	0:169	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	6	47	theme	single	1474:1479	arg1	scaffolds					1491:1499	single component scaffolds	1474:1499	single component scaffolds of PVA and PCL	1474:1514	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	9	48	theme	MTT	1883:1885	arg1	assay					1887:1891	MTT assay	1883:1891	MTT assay	1883:1891	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	10	49	theme	native	2148:2153	arg1	matrix					2169:2174	the native extracellular matrix	2144:2174	the native extracellular matrix	2144:2174	Our results provide the underlying insights needed to guide the design of the native extracellular matrix.					
31928136	1	50	theme	vinylalcohol	195:206	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	50	theme	vinylalcohol	195:206	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	9	51	theme	microscope	1906:1915	arg1	observations					1917:1928	inverted microscope observations	1897:1928	inverted microscope observations	1897:1928	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	6	52	theme	gelatin	1383:1389	arg1	incorporation					1353:1365	the incorporation	1349:1365	the incorporation of chitosan and gelatin	1349:1389	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	10	53	theme	matrix	2169:2174	arg1	design					2134:2139	the design	2130:2139	the design of the native extracellular matrix	2130:2174	Our results provide the underlying insights needed to guide the design of the native extracellular matrix.					
31928136	1	54	theme	scale	365:369	arg1	systems					371:377	several other single scale systems	344:377	several other single scale systems	344:377	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	6	55	theme	PCL	1512:1514	arg1	scaffolds					1491:1499	single component scaffolds	1474:1499	single component scaffolds of PVA and PCL	1474:1514	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	2	56	theme	PVA/CS	506:511	arg1	nanofibers					523:532	PVA/CS composite nanofibers	506:532	PVA/CS composite nanofibers (102 ± 52 nm)	506:546	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	2	56	theme	PVA/CS	506:511	arg1	102 ± 52 nm					535:545	102 ± 52 nm	535:545	102 ± 52 nm	535:545	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	9	57	theme	PVA/CS-PCL/Gel	1951:1964	arg1	membrane					1982:1989	the PVA/CS-PCL/Gel complex fibrous membrane	1947:1989	the PVA/CS-PCL/Gel complex fibrous membrane	1947:1989	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	3	58	theme	glutaraldehyde	784:797	arg1	vapor					799:803	glutaraldehyde vapor	784:803	glutaraldehyde vapor	784:803	The bi-component membrane was crosslinked by glutaraldehyde vapor to maintain its fiber morphology in the wet stage.					
31928136	6	59	theme	culture	1320:1326	arg1	results					1328:1334	Cell culture results	1315:1334	Cell culture results	1315:1334	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	9	60	theme	fibrous	1974:1980	arg1	membrane					1982:1989	the PVA/CS-PCL/Gel complex fibrous membrane	1947:1989	the PVA/CS-PCL/Gel complex fibrous membrane	1947:1989	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	5	61	theme	observations	1274:1285	arg1	assay					1287:1291	inverted microscope observations assay	1254:1291	inverted microscope observations assay	1254:1291	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	9	62	theme	single	2028:2033	arg1	scaffolds					2059:2067	other single component/scale systems scaffolds	2022:2067	other single component/scale systems scaffolds	2022:2067	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	4	63	theme	electrospun	903:913	arg1	membranes					915:923	the electrospun membranes	899:923	the electrospun membranes	899:923	Morphology, shrinkage and spectroscopic of the electrospun membranes were characterized.					
31928136	6	64	theme	cell	1405:1408	arg1	adhesion					1410:1417	cell adhesion	1405:1417	cell adhesion	1405:1417	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	1	65	theme	new	173:175	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	65	theme	new	173:175	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	6	66	theme	PVA	1504:1506	arg1	scaffolds					1491:1499	single component scaffolds	1474:1499	single component scaffolds of PVA and PCL	1474:1514	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	5	67	attach	derived	1034:1040	arg2	MSCs					1028:1031	MSCs	1028:1031	MSCs	1028:1031	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	67	attach	derived	1034:1040	arg2	cells					1021:1025	mesenchymal stem cells	1004:1025	mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel)	1004:1135	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	67	attach	derived	1034:1040	arg1	rabbit					1047:1052	rabbit	1047:1052	rabbit	1047:1052	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	2	68	from	matrix	414:419	arg1	structure					440:448	structure	440:448	structure	440:448	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	2	68	from	matrix	414:419	arg1	composition					424:434	composition	424:434	composition	424:434	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	2	69	theme	bi-component	704:715	arg1	membrane					729:736	a bi-component multi-scale membrane	702:736	a bi-component multi-scale membrane	702:736	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	8	70	theme	scaffolds	1793:1801	arg1	surface					1778:1784	the surface	1774:1784	the surface of the scaffolds	1774:1801	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	1	71	theme	poly	190:193	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	71	theme	poly	190:193	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	9	72	theme	other	2022:2026	arg1	scaffolds					2059:2067	other single component/scale systems scaffolds	2022:2067	other single component/scale systems scaffolds	2022:2067	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	5	73	theme	inverted	1254:1261	arg1	assay					1287:1291	inverted microscope observations assay	1254:1291	inverted microscope observations assay	1254:1291	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	1	74	theme	single	358:363	arg1	systems					371:377	several other single scale systems	344:377	several other single scale systems	344:377	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	0	75	theme	enhanced	121:128	arg1	infiltration					139:150	enhanced cellular infiltration	121:150	enhanced cellular infiltration	121:150	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	5	76	theme	multiscale	973:982	arg1	membrane					984:991	the newly developed multiscale membrane	953:991	the newly developed multiscale membrane	953:991	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	9	77	theme	component/scale	2035:2049	arg1	scaffolds					2059:2067	other single component/scale systems scaffolds	2022:2067	other single component/scale systems scaffolds	2022:2067	MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds.					
31928136	1	78	dep	multiscale	266:275	arg1	e-caprolactone					232:245	e-caprolactone	232:245	e-caprolactone	232:245	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	78	dep	multiscale	266:275	arg1	-poly					226:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly	171:230	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)	171:246	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	1	79	theme	other	352:356	arg1	systems					371:377	several other single scale systems	344:377	several other single scale systems	344:377	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	5	80	theme	fiber	1074:1078	arg1	PCL/Gel					1109:1115	PCL/Gel	1109:1115	PCL/Gel	1109:1115	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	80	theme	fiber	1074:1078	arg1	PVA					1091:1093	PVA	1091:1093	PVA	1091:1093	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	80	theme	fiber	1074:1078	arg1	PVA/CS					1096:1101	PVA/CS	1096:1101	PVA/CS	1096:1101	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	80	theme	fiber	1074:1078	arg1	PVA/CS-PCL/Gel					1121:1134	PVA/CS-PCL/Gel	1121:1134	PVA/CS-PCL/Gel	1121:1134	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	80	theme	fiber	1074:1078	arg1	scaffolds					1080:1088	five different fiber scaffolds	1059:1088	five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel)	1059:1135	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	80	theme	fiber	1074:1078	arg1	PCL					1104:1106	PCL	1104:1106	PCL	1104:1106	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	2	81	theme	extracellular	400:412	arg1	matrix					414:419	the native extracellular matrix	389:419	the native extracellular matrix in composition and structure	389:448	To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane.					
31928136	0	82	theme	multi-scale	51:61	arg1	scaffold					75:82	PVA/CS-PCL/gel multi-scale electrospun scaffold	36:82	PVA/CS-PCL/gel multi-scale electrospun scaffold	36:82	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	0	83	theme	scaffold	75:82	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	0	83	theme	scaffold	75:82	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of PVA/CS-PCL/gel multi-scale electrospun scaffold: simulating extracellular matrix for enhanced cellular infiltration and proliferation.					
31928136	1	84	theme	electrospun	277:287	arg1	scaffold					289:296	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold	171:296	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold	171:296	A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems.					
31928136	7	85	theme	membrane	1547:1554	arg1	scaffolds					1556:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds	1517:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds	1517:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	8	86	theme	microscopy	1675:1684	arg1	observations					1692:1703	Scanning electron microscopy (SEM) observations	1657:1703	Scanning electron microscopy (SEM) observations	1657:1703	Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture.					
31928136	7	87	theme	multiscale	1521:1530	arg1	scaffolds					1556:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds	1517:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds	1517:1564	The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells.					
31928136	10	88	theme	underlying	2094:2103	arg1	insights					2105:2112	the underlying insights	2090:2112	the underlying insights needed to guide the design of the native extracellular matrix	2090:2174	Our results provide the underlying insights needed to guide the design of the native extracellular matrix.					
31928136	5	89	dep	scaffolds	1080:1088	arg1	PCL/Gel					1109:1115	PCL/Gel	1109:1115	PCL/Gel	1109:1115	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	89	dep	scaffolds	1080:1088	arg1	PVA					1091:1093	PVA	1091:1093	PVA	1091:1093	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	89	dep	scaffolds	1080:1088	arg1	PVA/CS					1096:1101	PVA/CS	1096:1101	PVA/CS	1096:1101	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	89	dep	scaffolds	1080:1088	arg1	PVA/CS-PCL/Gel					1121:1134	PVA/CS-PCL/Gel	1121:1134	PVA/CS-PCL/Gel	1121:1134	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	89	dep	scaffolds	1080:1088	arg1	scaffolds					1080:1088	five different fiber scaffolds	1059:1088	five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel)	1059:1135	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	89	dep	scaffolds	1080:1088	arg1	PCL					1104:1106	PCL	1104:1106	PCL	1104:1106	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	90	theme	stem	1016:1019	arg1	MSCs					1028:1031	MSCs	1028:1031	MSCs	1028:1031	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	5	90	theme	stem	1016:1019	arg1	cells					1021:1025	mesenchymal stem cells	1004:1025	mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel)	1004:1135	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
31928136	6	91	theme	component	1481:1489	arg1	scaffolds					1491:1499	single component scaffolds	1474:1499	single component scaffolds of PVA and PCL	1474:1514	Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL.					
31928136	5	92	theme	electron	1233:1240	arg1	microscopy					1242:1251	scanning electron microscopy	1224:1251	scanning electron microscopy	1224:1251	To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric.					
32439435	0	0	theme	different	99:107	arg1	sources					119:125	different botanical sources	99:125	different botanical sources	99:125	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	1	1	theme	pharmacological	363:377	arg1	effects					379:385	their in vivo pharmacological effects	349:385	their in vivo pharmacological effects in an inflammation model	349:410	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	2	theme	tripolyphosphate	182:197	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	2	theme	tripolyphosphate	182:197	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	2	theme	tripolyphosphate	182:197	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	2	theme	tripolyphosphate	182:197	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	2	theme	tripolyphosphate	182:197	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	2	theme	tripolyphosphate	182:197	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	3	theme	wall	270:273	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	3	theme	wall	270:273	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	3	theme	wall	270:273	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	3	theme	wall	270:273	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	3	theme	wall	270:273	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	3	theme	wall	270:273	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	5	4	theme	Freund	1259:1264	arg1	model					1283:1287	complete Freund's adjuvant (CFA) model	1250:1287	complete Freund's adjuvant (CFA) model	1250:1287	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	1	5	theme	cross-linked	199:210	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	5	theme	cross-linked	199:210	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	5	theme	cross-linked	199:210	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	5	theme	cross-linked	199:210	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	5	theme	cross-linked	199:210	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	5	theme	cross-linked	199:210	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	5	6	theme	antihyperalgesic	1183:1198	arg1	effects					1200:1206	the antihyperalgesic effects	1179:1206	the antihyperalgesic effects	1179:1206	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	4	7	theme	encapsulation	1064:1076	arg1	efficiency					1078:1087	C-phycocyanin encapsulation efficiency	1050:1087	C-phycocyanin encapsulation efficiency (67.58%)	1050:1096	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	7	theme	encapsulation	1064:1076	arg1	%					1095:1095	67.58%	1090:1095	67.58%	1090:1095	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	3	8	theme	amorphous	826:834	arg1	chains					836:841	amorphous chains	826:841	amorphous chains of cross-linked starches	826:866	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	6	9	dep	in	1481:1482	arg1	vivo					1484:1487	vivo	1484:1487	vivo	1484:1487	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	4	10	theme	cross-linked	902:913	arg1	starch					922:927	the cross-linked potato starch	898:927	the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus	898:1102	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	0	11	theme	botanical	109:117	arg1	sources					119:125	different botanical sources	99:125	different botanical sources	99:125	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	3	12	theme	cross-linked	569:580	arg1	composites					605:614	cross-linked starches-C-phycocyanin composites	569:614	cross-linked starches-C-phycocyanin composites	569:614	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	3	13	link	cross-linked	846:857	arg1	starches					859:866	cross-linked starches	846:866	cross-linked starches	846:866	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	1	14	theme	C-phycocyanin	289:301	arg1	encapsulation					303:315	C-phycocyanin encapsulation	289:315	C-phycocyanin encapsulation	289:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	15	dep	in	355:356	arg1	vivo					358:361	vivo	358:361	vivo	358:361	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	3	16	theme	scanning	619:626	arg1	microscopy					637:646	scanning electron microscopy	619:646	scanning electron microscopy	619:646	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	3	17	theme	cross-linked	846:857	arg1	starches					859:866	cross-linked starches	846:866	cross-linked starches	846:866	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	3	18	theme	electron	628:635	arg1	microscopy					637:646	scanning electron microscopy	619:646	scanning electron microscopy	619:646	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	7	19	theme	chemical	1517:1524	arg1	modification					1526:1537	chemical modification	1517:1537	chemical modification of starches followed by physical treatment	1517:1580	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	3	20	theme	starches	859:866	arg1	chains					836:841	amorphous chains	826:841	amorphous chains of cross-linked starches	826:866	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	6	21	from	state	1367:1371	arg1	formation					1324:1332	the formation	1320:1332	the formation of a hydrogel network in swollen state	1320:1371	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	7	22	theme	starches	1542:1549	arg1	modification					1526:1537	chemical modification	1517:1537	chemical modification of starches followed by physical treatment	1517:1580	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	1	23	theme	inflammation	393:404	arg1	model					406:410	an inflammation model	390:410	an inflammation model	390:410	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	2	24	theme	cross-linked	417:428	arg1	starches					430:437	The cross-linked starches	413:437	The cross-linked starches	413:437	The cross-linked starches were successfully obtained, characterized, and submitted to C-phycocyanin encapsulation by freeze-drying.					
32439435	4	25	theme	substitution	981:992	arg1	0.004					1002:1006	0.004	1002:1006	0.004	1002:1006	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	25	theme	substitution	981:992	arg1	degree					994:999	substitution degree	981:999	substitution degree (0.004)	981:1007	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	7	26	theme	modification	1526:1537	arg1	combination					1502:1512	The combination	1498:1512	The combination of chemical modification of starches followed by physical treatment	1498:1580	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	3	27	link	cross-linked	569:580	arg1	composites					605:614	cross-linked starches-C-phycocyanin composites	569:614	cross-linked starches-C-phycocyanin composites	569:614	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	5	28	theme	cross-linked	1128:1139	arg1	starch-C-phycocyanin					1148:1167	The cross-linked potato starch-C-phycocyanin	1124:1167	The cross-linked potato starch-C-phycocyanin	1124:1167	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	6	29	theme	swollen	1359:1365	arg1	state					1367:1371	swollen state	1359:1371	swollen state	1359:1371	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	1	30	link	cross-linked	199:210	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	30	link	cross-linked	199:210	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	30	link	cross-linked	199:210	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	30	link	cross-linked	199:210	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	30	link	cross-linked	199:210	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	30	link	cross-linked	199:210	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	2	31	link	cross-linked	417:428	arg1	starches					430:437	The cross-linked starches	413:437	The cross-linked starches	413:437	The cross-linked starches were successfully obtained, characterized, and submitted to C-phycocyanin encapsulation by freeze-drying.					
32439435	3	32	theme	starches-C-phycocyanin	582:603	arg1	composites					605:614	cross-linked starches-C-phycocyanin composites	569:614	cross-linked starches-C-phycocyanin composites	569:614	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	3	33	theme	composites	605:614	arg1	characterization					549:564	The characterization	545:564	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry	545:763	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	1	34	from	effects	379:385	arg1	model					406:410	an inflammation model	390:410	an inflammation model	390:410	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	5	35	theme	adjuvant	1268:1275	arg1	Freund					1259:1264	complete Freund's adjuvant (CFA)	1250:1281	complete Freund's adjuvant (CFA) model	1250:1287	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	5	35	theme	adjuvant	1268:1275	arg1	CFA					1278:1280	CFA	1278:1280	CFA	1278:1280	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	7	36	theme	useful	1604:1609	arg1	itself					1592:1597	itself	1592:1597	itself	1592:1597	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	7	36	theme	useful	1604:1609	arg1	tool					1611:1614	a useful tool	1602:1614	a useful tool for the development of pharmaceutical formulations	1602:1665	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	0	37	theme	C-phycocyanin	36:48	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	0	37	theme	C-phycocyanin	36:48	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	5	38	link	cross-linked	1128:1139	arg1	starch-C-phycocyanin					1148:1167	The cross-linked potato starch-C-phycocyanin	1124:1167	The cross-linked potato starch-C-phycocyanin	1124:1167	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	3	39	theme	scanning	744:751	arg1	calorimetry					753:763	differential scanning calorimetry	731:763	differential scanning calorimetry	731:763	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	0	40	theme	STMP/STPP	62:70	arg1	starches					85:92	STMP/STPP cross-linked starches	62:92	STMP/STPP cross-linked starches from different botanical sources	62:125	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	6	41	theme	in	1481:1482	arg1	effects					1489:1495	long-lasting in vivo effects	1468:1495	long-lasting in vivo effects	1468:1495	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	4	42	theme	uptake	1016:1021	arg1	capacity					1023:1030	water uptake capacity	1010:1030	water uptake capacity (0.88 g g-1)	1010:1043	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	42	theme	uptake	1016:1021	arg1	0.88 g g-1					1033:1042	0.88 g g-1	1033:1042	0.88 g g-1	1033:1042	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	7	43	theme	formulations	1654:1665	arg1	development					1624:1634	the development	1620:1634	the development of pharmaceutical formulations	1620:1665	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	6	44	theme	network	1348:1354	arg1	formation					1324:1332	the formation	1320:1332	the formation of a hydrogel network in swollen state	1320:1371	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	7	45	theme	physical	1563:1570	arg1	treatment					1572:1580	physical treatment	1563:1580	physical treatment	1563:1580	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	6	46	from	network	1348:1354	arg1	state					1367:1371	swollen state	1359:1371	swollen state	1359:1371	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	2	47	theme	C-phycocyanin	499:511	arg1	encapsulation					513:525	C-phycocyanin encapsulation	499:525	C-phycocyanin encapsulation	499:525	The cross-linked starches were successfully obtained, characterized, and submitted to C-phycocyanin encapsulation by freeze-drying.					
32439435	4	48	theme	water	1010:1014	arg1	capacity					1023:1030	water uptake capacity	1010:1030	water uptake capacity (0.88 g g-1)	1010:1043	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	48	theme	water	1010:1014	arg1	0.88 g g-1					1033:1042	0.88 g g-1	1033:1042	0.88 g g-1	1033:1042	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	5	49	theme	potato	1141:1146	arg1	starch-C-phycocyanin					1148:1167	The cross-linked potato starch-C-phycocyanin	1124:1167	The cross-linked potato starch-C-phycocyanin	1124:1167	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	3	50	theme	X-ray	690:694	arg1	diffraction					696:706	X-ray diffraction	690:706	X-ray diffraction	690:706	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	7	51	theme	pharmaceutical	1639:1652	arg1	formulations					1654:1665	pharmaceutical formulations	1639:1665	pharmaceutical formulations	1639:1665	The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.					
32439435	6	52	theme	hydrogel	1339:1346	arg1	network					1348:1354	a hydrogel network	1337:1354	a hydrogel network in swollen state	1337:1371	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	1	53	theme	in	355:356	arg1	effects					379:385	their in vivo pharmacological effects	349:385	their in vivo pharmacological effects in an inflammation model	349:410	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	0	54	link	cross-linked	72:83	arg1	starches					85:92	STMP/STPP cross-linked starches	62:92	STMP/STPP cross-linked starches from different botanical sources	62:125	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	4	55	link	cross-linked	902:913	arg1	starch					922:927	the cross-linked potato starch	898:927	the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus	898:1102	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	56	theme	potato	915:920	arg1	starch					922:927	the cross-linked potato starch	898:927	the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus	898:1102	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	0	57	from	sources	119:125	arg1	starches					85:92	STMP/STPP cross-linked starches	62:92	STMP/STPP cross-linked starches from different botanical sources	62:125	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	3	58	theme	differential	731:742	arg1	calorimetry					753:763	differential scanning calorimetry	731:763	differential scanning calorimetry	731:763	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	3	59	dep	transform	657:665	arg1	infrared					667:674	infrared	667:674	transform infrared spectroscopy	657:687	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	6	60	theme	long-lasting	1468:1479	arg1	effects					1489:1495	long-lasting in vivo effects	1468:1495	long-lasting in vivo effects	1468:1495	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	1	61	theme	breadfruit	247:256	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	61	theme	breadfruit	247:256	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	61	theme	breadfruit	247:256	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	61	theme	breadfruit	247:256	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	61	theme	breadfruit	247:256	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	61	theme	breadfruit	247:256	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	4	62	theme	phosphorous	951:961	arg1	content					963:969	the highest phosphorous content	939:969	the highest phosphorous content (0.084%)	939:978	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	62	theme	phosphorous	951:961	arg1	%					977:977	0.084%	972:977	0.084%	972:977	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	3	63	dep	Fourier	649:655	arg1	transform					657:665	transform	657:665	transform infrared spectroscopy	657:687	The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches.					
32439435	1	64	theme	sodium	151:156	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	64	theme	sodium	151:156	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	64	theme	sodium	151:156	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	64	theme	sodium	151:156	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	64	theme	sodium	151:156	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	64	theme	sodium	151:156	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	6	65	theme	Starch	1290:1295	arg1	cross-linking					1297:1309	Starch cross-linking	1290:1309	Starch cross-linking	1290:1309	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	0	66	theme	cross-linked	72:83	arg1	starches					85:92	STMP/STPP cross-linked starches	62:92	STMP/STPP cross-linked starches from different botanical sources	62:125	Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.					
32439435	5	67	theme	complete	1250:1257	arg1	Freund					1259:1264	complete Freund's adjuvant (CFA)	1250:1281	complete Freund's adjuvant (CFA) model	1250:1287	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	5	67	theme	complete	1250:1257	arg1	CFA					1278:1280	CFA	1278:1280	CFA	1278:1280	The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model.					
32439435	4	68	theme	highest	943:949	arg1	content					963:969	the highest phosphorous content	939:969	the highest phosphorous content (0.084%)	939:978	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	68	theme	highest	943:949	arg1	%					977:977	0.084%	972:977	0.084%	972:977	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	1	69	theme	trimetaphosphate/sodium	158:180	arg1	banana					220:225	banana	220:225	banana	220:225	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	69	theme	trimetaphosphate/sodium	158:180	arg1	starches					258:265	breadfruit starches	247:265	breadfruit starches	247:265	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	69	theme	trimetaphosphate/sodium	158:180	arg1	corn					228:231	corn	228:231	corn	228:231	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	69	theme	trimetaphosphate/sodium	158:180	arg1	cassava					234:240	cassava	234:240	cassava	234:240	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	69	theme	trimetaphosphate/sodium	158:180	arg1	materials					275:283	wall materials	270:283	wall materials for C-phycocyanin encapsulation	270:315	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	1	69	theme	trimetaphosphate/sodium	158:180	arg1	potato					212:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato	151:217	This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model.					
32439435	6	70	from	formation	1324:1332	arg1	state					1367:1371	swollen state	1359:1371	swollen state	1359:1371	Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects.					
32439435	4	71	theme	C-phycocyanin	1050:1062	arg1	efficiency					1078:1087	C-phycocyanin encapsulation efficiency	1050:1087	C-phycocyanin encapsulation efficiency (67.58%)	1050:1096	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
32439435	4	71	theme	C-phycocyanin	1050:1062	arg1	%					1095:1095	67.58%	1090:1095	67.58%	1090:1095	Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g-1), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo.					
33858582	8	0	theme	ionic	1025:1029	arg1	hydrogels					1049:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	8	1	theme	starch/poly	1013:1023	arg1	hydrogels					1049:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	1	2	theme	first	128:132	arg1	time					134:137	the first time	124:137	the first time	124:137	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	1	3	theme	eutectic	213:220	arg1	solvent					222:228	a polymerizable deep eutectic solvent	192:228	a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	192:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	1	3	theme	eutectic	213:220	arg1	DES					231:233	DES	231:233	DES	231:233	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	5	4	theme	pure	614:617	arg1	hydrogel					638:645	the pure poly(ionic liquid) hydrogel	610:645	the pure poly(ionic liquid) hydrogel	610:645	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	7	5	theme	interfacial	936:946	arg1	interaction					948:958	a strong interfacial interaction	927:958	a strong interfacial interaction between starch and the polymer hydrogel network	927:1006	The main reason was that there was a strong interfacial interaction between starch and the polymer hydrogel network.					
33858582	0	6	theme	deep	70:73	arg1	solvent					84:90	deep eutectic solvent	70:90	deep eutectic solvent	70:90	Starch as a reinforcement agent for poly(ionic liquid) hydrogels from deep eutectic solvent via frontal polymerization.					
33858582	5	7	theme	poly	619:622	arg1	hydrogel					638:645	the pure poly(ionic liquid) hydrogel	610:645	the pure poly(ionic liquid) hydrogel	610:645	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	8	8	theme	liquid	1031:1036	arg1	hydrogels					1049:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	7	9	theme	hydrogel	991:998	arg1	network					1000:1006	the polymer hydrogel network	979:1006	the polymer hydrogel network	979:1006	The main reason was that there was a strong interfacial interaction between starch and the polymer hydrogel network.					
33858582	2	10	from	solubility	286:295	arg1	DES					357:359	the polymerizable DES	339:359	the polymerizable DES	339:359	The solubility and dispersibility for starch granules in the polymerizable DES was investigated.					
33858582	3	11	theme	electrical	452:461	arg1	conductivity					463:474	electrical conductivity	452:474	electrical conductivity of composite hydrogels	452:497	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	6	12	theme	fixed	796:800	arg1	strain					802:807	the fixed strain	792:807	the fixed strain	792:807	When the fixed strain was 80 %, its compressive strength could increase by 6 times and reaches 16.8 MPa.					
33858582	6	12	theme	fixed	796:800	arg1	%					816:816	80 %	813:816	80 %	813:816	When the fixed strain was 80 %, its compressive strength could increase by 6 times and reaches 16.8 MPa.					
33858582	3	13	theme	hydrogels	489:497	arg1	properties					437:446	mechanical properties	426:446	mechanical properties	426:446	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	3	13	theme	hydrogels	489:497	arg1	conductivity					463:474	electrical conductivity	452:474	electrical conductivity of composite hydrogels	452:497	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	3	13	theme	hydrogels	489:497	arg1	behaviors					415:423	FP behaviors	412:423	FP behaviors	412:423	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	5	14	theme	K	781:781	arg1	Pa.					783:785	126 K Pa.	777:785	126 K Pa.	777:785	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	2	15	theme	polymerizable	343:355	arg1	DES					357:359	the polymerizable DES	339:359	the polymerizable DES	339:359	The solubility and dispersibility for starch granules in the polymerizable DES was investigated.					
33858582	0	16	theme	eutectic	75:82	arg1	solvent					84:90	deep eutectic solvent	70:90	deep eutectic solvent	70:90	Starch as a reinforcement agent for poly(ionic liquid) hydrogels from deep eutectic solvent via frontal polymerization.					
33858582	2	17	from	dispersibility	301:314	arg1	DES					357:359	the polymerizable DES	339:359	the polymerizable DES	339:359	The solubility and dispersibility for starch granules in the polymerizable DES was investigated.					
33858582	1	18	theme	conductive	140:149	arg1	hydrogels					177:185	conductive starch/poly(ionic liquid) hydrogels	140:185	conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	140:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	5	19	theme	composite	716:724	arg1	hydrogel					726:733	composite hydrogel	716:733	composite hydrogel	716:733	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	5	20	theme	K	685:685	arg1	Pa					687:688	41 K Pa	682:688	41 K Pa	682:688	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	5	20	theme	K	685:685	arg1	strength					669:676	the tensile strength	657:676	the tensile strength	657:676	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	1	21	theme	starch/poly	151:161	arg1	hydrogels					177:185	conductive starch/poly(ionic liquid) hydrogels	140:185	conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	140:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	5	22	theme	hydrogel	726:733	arg1	strength					704:711	the tensile strength	692:711	the tensile strength of composite hydrogel	692:733	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	5	23	dep	increased	741:749	arg1	could					735:739	could	735:739	could	735:739	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	0	24	theme	reinforcement	12:24	arg1	agent					26:30	a reinforcement agent	10:30	a reinforcement agent for poly(ionic liquid)	10:53	Starch as a reinforcement agent for poly(ionic liquid) hydrogels from deep eutectic solvent via frontal polymerization.					
33858582	0	25	theme	frontal	96:102	arg1	polymerization					104:117	frontal polymerization	96:117	frontal polymerization	96:117	Starch as a reinforcement agent for poly(ionic liquid) hydrogels from deep eutectic solvent via frontal polymerization.					
33858582	2	26	dep	solubility	286:295	arg1	The					282:284	The	282:284	The	282:284	The solubility and dispersibility for starch granules in the polymerizable DES was investigated.					
33858582	3	27	theme	FP	412:413	arg1	behaviors					415:423	FP behaviors	412:423	FP behaviors	412:423	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	3	28	from	effects	383:389	arg1	properties					437:446	mechanical properties	426:446	mechanical properties	426:446	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	3	28	from	effects	383:389	arg1	conductivity					463:474	electrical conductivity	452:474	electrical conductivity of composite hydrogels	452:497	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	3	28	from	effects	383:389	arg1	behaviors					415:423	FP behaviors	412:423	FP behaviors	412:423	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	1	29	theme	frontal	239:245	arg1	FP					263:264	FP	263:264	FP	263:264	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	1	29	theme	frontal	239:245	arg1	polymerization					247:260	frontal polymerization	239:260	frontal polymerization (FP)	239:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	9	30	theme	water	1112:1116	arg1	Absorption					1098:1107	Absorption	1098:1107	Absorption of water	1098:1116	Absorption of water could increase the conductivity of the composite hydrogel significantly.					
33858582	1	31	theme	liquid	169:174	arg1	hydrogels					177:185	conductive starch/poly(ionic liquid) hydrogels	140:185	conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	140:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	8	32	theme	good	1068:1071	arg1	conductivity					1084:1095	good electrical conductivity	1068:1095	good electrical conductivity	1068:1095	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	3	33	theme	content	401:407	arg1	effects					383:389	The effects	379:389	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels	379:497	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	5	34	theme	tensile	696:702	arg1	strength					704:711	the tensile strength	692:711	the tensile strength of composite hydrogel	692:733	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	1	35	from	solvent	222:228	arg1	hydrogels					177:185	conductive starch/poly(ionic liquid) hydrogels	140:185	conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	140:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	8	36	theme	electrical	1073:1082	arg1	conductivity					1084:1095	good electrical conductivity	1068:1095	good electrical conductivity	1068:1095	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	7	37	theme	main	896:899	arg1	reason					901:906	The main reason	892:906	The main reason	892:906	The main reason was that there was a strong interfacial interaction between starch and the polymer hydrogel network.					
33858582	8	38	theme	composite	1039:1047	arg1	hydrogels					1049:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	5	39	theme	tensile	661:667	arg1	Pa					687:688	41 K Pa	682:688	41 K Pa	682:688	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	5	39	theme	tensile	661:667	arg1	strength					669:676	the tensile strength	657:676	the tensile strength	657:676	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	5	40	theme	ionic	624:628	arg1	hydrogel					638:645	the pure poly(ionic liquid) hydrogel	610:645	the pure poly(ionic liquid) hydrogel	610:645	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	7	41	theme	strong	929:934	arg1	interaction					948:958	a strong interfacial interaction	927:958	a strong interfacial interaction between starch and the polymer hydrogel network	927:1006	The main reason was that there was a strong interfacial interaction between starch and the polymer hydrogel network.					
33858582	6	42	theme	compressive	823:833	arg1	strength					835:842	its compressive strength	819:842	its compressive strength	819:842	When the fixed strain was 80 %, its compressive strength could increase by 6 times and reaches 16.8 MPa.					
33858582	5	43	theme	liquid	630:635	arg1	hydrogel					638:645	the pure poly(ionic liquid) hydrogel	610:645	the pure poly(ionic liquid) hydrogel	610:645	Comparing with the pure poly(ionic liquid) hydrogel from DES (the tensile strength was 41 K Pa), the tensile strength of composite hydrogel could increased by 3.07 times and reached 126 K Pa.					
33858582	7	44	theme	polymer	983:989	arg1	network					1000:1006	the polymer hydrogel network	979:1006	the polymer hydrogel network	979:1006	The main reason was that there was a strong interfacial interaction between starch and the polymer hydrogel network.					
33858582	3	45	theme	mechanical	426:435	arg1	properties					437:446	mechanical properties	426:446	mechanical properties	426:446	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	0	46	theme	liquid	47:52	arg1	poly					36:39	poly	36:39	poly(ionic liquid)	36:53	Starch as a reinforcement agent for poly(ionic liquid) hydrogels from deep eutectic solvent via frontal polymerization.					
33858582	9	47	theme	composite	1157:1165	arg1	hydrogel					1167:1174	the composite hydrogel	1153:1174	the composite hydrogel	1153:1174	Absorption of water could increase the conductivity of the composite hydrogel significantly.					
33858582	1	48	theme	ionic	163:167	arg1	hydrogels					177:185	conductive starch/poly(ionic liquid) hydrogels	140:185	conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	140:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	2	49	theme	starch	320:325	arg1	granules					327:334	starch granules	320:334	starch granules	320:334	The solubility and dispersibility for starch granules in the polymerizable DES was investigated.					
33858582	3	50	theme	starch	394:399	arg1	content					401:407	starch content	394:407	starch content	394:407	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	1	51	theme	polymerizable	194:206	arg1	solvent					222:228	a polymerizable deep eutectic solvent	192:228	a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	192:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	1	51	theme	polymerizable	194:206	arg1	DES					231:233	DES	231:233	DES	231:233	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	9	52	theme	hydrogel	1167:1174	arg1	conductivity					1137:1148	the conductivity	1133:1148	the conductivity of the composite hydrogel	1133:1174	Absorption of water could increase the conductivity of the composite hydrogel significantly.					
33858582	8	53	contain	had	1064:1066	arg2	conductivity					1084:1095	good electrical conductivity	1068:1095	good electrical conductivity	1068:1095	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	8	53	contain	had	1064:1066	arg1	hydrogels					1049:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels	1009:1057	The starch/poly(ionic liquid) composite hydrogels also had good electrical conductivity.					
33858582	3	54	theme	composite	479:487	arg1	hydrogels					489:497	composite hydrogels	479:497	composite hydrogels	479:497	The effects of starch content on FP behaviors, mechanical properties and electrical conductivity of composite hydrogels were studied.					
33858582	1	55	theme	deep	208:211	arg1	solvent					222:228	a polymerizable deep eutectic solvent	192:228	a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP)	192:265	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33858582	1	55	theme	deep	208:211	arg1	DES					231:233	DES	231:233	DES	231:233	For the first time, conductive starch/poly(ionic liquid) hydrogels from a polymerizable deep eutectic solvent (DES) by frontal polymerization (FP) were reported.					
33384130	1	0	from	milk	155:158	arg1	present					144:150	present	144:150	present	144:150	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	7	1	theme	detection	1158:1166	arg1	limit					1168:1172	a detection limit	1156:1172	a detection limit of 0.88 nmol L-1	1156:1189	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	2	dep	concentration	1100:1112	arg1	1.0-10 nmol L-1					1115:1129	1.0-10 nmol L-1	1115:1129	1.0-10 nmol L-1	1115:1129	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	2	dep	concentration	1100:1112	arg1	25-125 nmol L-1					1135:1149	25-125 nmol L-1	1135:1149	25-125 nmol L-1	1135:1149	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	4	3	theme	graphite	536:543	arg1	PE					562:563	PE	562:563	PE	562:563	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	4	3	theme	graphite	536:543	arg1	electrode					551:559	graphite paper electrode	536:559	graphite paper electrode (PE)	536:564	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	6	4	theme	Plackett-Burman	838:852	arg1	methods					829:835	multivariate methods	816:835	multivariate methods (Plackett-Burman design and central composite design)	816:889	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	4	theme	Plackett-Burman	838:852	arg1	design					854:859	Plackett-Burman design	838:859	Plackett-Burman design	838:859	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	5	theme	detection	721:729	arg1	conditions					731:740	the detection conditions	717:740	the detection conditions	717:740	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	1	6	theme	present	144:150	arg1	disaccharide					116:127	a disaccharide	114:127	a disaccharide	114:127	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	1	6	theme	present	144:150	arg1	Lactose					97:103	Lactose	97:103	Lactose (LAC)	97:109	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	5	7	from	presence	653:660	arg1	PPy/PE					696:701	PPy/PE	696:701	PPy/PE	696:701	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	5	7	from	presence	653:660	arg1	PE					692:693	PE	692:693	PE (PPy/PE)	692:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	6	8	theme	multivariate	816:827	arg1	methods					829:835	multivariate methods	816:835	multivariate methods (Plackett-Burman design and central composite design)	816:889	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	8	theme	multivariate	816:827	arg1	design					854:859	Plackett-Burman design	838:859	Plackett-Burman design	838:859	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	8	theme	multivariate	816:827	arg1	design					883:888	central composite design	865:888	central composite design	865:888	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	1	9	from	present	144:150	arg1	milk					155:158	milk	155:158	milk	155:158	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	1	9	from	present	144:150	arg1	products					170:177	dairy products	164:177	dairy products	164:177	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	3	10	theme	present	315:321	arg1	study					323:327	The present study	311:327	The present study	311:327	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	6	11	dep	methods	829:835	arg1	methods					829:835	multivariate methods	816:835	multivariate methods (Plackett-Burman design and central composite design)	816:889	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	11	dep	methods	829:835	arg1	design					854:859	Plackett-Burman design	838:859	Plackett-Burman design	838:859	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	11	dep	methods	829:835	arg1	design					883:888	central composite design	865:888	central composite design	865:888	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	7	12	theme	high	1032:1035	arg1	sensitivity					1037:1047	high sensitivity	1032:1047	high sensitivity	1032:1047	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	2	13	theme	food	255:258	arg1	industries					260:269	food industries	255:269	food industries	255:269	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	5	14	from	electrosynthesis	608:623	arg1	PE					692:693	PE	692:693	PE (PPy/PE)	692:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	5	14	from	electrosynthesis	608:623	arg1	PPy/PE					696:701	PPy/PE	696:701	PPy/PE	696:701	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	5	14	from	electrosynthesis	608:623	arg1	presence					653:660	the presence	649:660	the presence of LAC (template molecule) on PE (PPy/PE)	649:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	4	15	theme	paper	545:549	arg1	PE					562:563	PE	562:563	PE	562:563	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	4	15	theme	paper	545:549	arg1	electrode					551:559	graphite paper electrode	536:559	graphite paper electrode (PE)	536:564	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	7	16	theme	concentration	1100:1112	arg1	ranges					1090:1095	two dynamic linear ranges	1071:1095	two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1)	1071:1150	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	16	theme	concentration	1100:1112	arg1	limit					1168:1172	a detection limit	1156:1172	a detection limit of 0.88 nmol L-1	1156:1189	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	8	17	theme	selective	1214:1222	arg1	recognition					1234:1244	selective molecular recognition	1214:1244	selective molecular recognition for LAC	1214:1252	The MIP sensor showed selective molecular recognition for LAC in the presence of structurally related molecules.					
33384130	8	18	theme	related	1286:1292	arg1	molecules					1294:1302	structurally related molecules	1273:1302	structurally related molecules	1273:1302	The MIP sensor showed selective molecular recognition for LAC in the presence of structurally related molecules.					
33384130	5	19	theme	template	670:677	arg1	molecule					679:686	LAC (template molecule)	665:687	LAC (template molecule)	665:687	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	1	20	attach	present	144:150	arg2	Lactose					97:103	Lactose	97:103	Lactose (LAC)	97:109	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	1	20	attach	present	144:150	arg1	milk					155:158	milk	155:158	milk	155:158	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	1	20	attach	present	144:150	arg1	products					170:177	dairy products	164:177	dairy products	164:177	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	1	20	attach	present	144:150	arg2	disaccharide					116:127	a disaccharide	114:127	a disaccharide	114:127	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	5	21	theme	MIP	571:573	arg1	film					575:578	The MIP film	567:578	The MIP film	567:578	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	2	22	theme	abnormalities	238:250	arg1	indicator					208:216	an important indicator	195:216	an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health	195:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	2	22	theme	abnormalities	238:250	arg1	content					184:190	LAC content	180:190	LAC content	180:190	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	2	23	from	indicator	208:216	arg1	industries					260:269	food industries	255:269	food industries	255:269	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	2	23	from	indicator	208:216	arg1	health					303:308	human and animal health	286:308	human and animal health	286:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	3	24	theme	sensitive	404:412	arg1	detection					414:422	sensitive detection	404:422	sensitive detection of LAC	404:429	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	0	25	theme	lactose	14:20	arg1	sensor					44:49	Non-enzymatic lactose molecularly imprinted sensor	0:49	Non-enzymatic lactose molecularly imprinted sensor	0:49	Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode.					
33384130	5	26	theme	molecule	679:686	arg1	presence					653:660	the presence	649:660	the presence of LAC (template molecule) on PE (PPy/PE)	649:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	3	27	theme	voltammetric	380:391	arg1	sensor					393:398	an innovative imprinted voltammetric sensor	356:398	an innovative imprinted voltammetric sensor for sensitive detection of LAC	356:429	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	0	28	theme	Non-enzymatic	0:12	arg1	sensor					44:49	Non-enzymatic lactose molecularly imprinted sensor	0:49	Non-enzymatic lactose molecularly imprinted sensor	0:49	Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode.					
33384130	8	29	theme	molecules	1294:1302	arg1	presence					1261:1268	the presence	1257:1268	the presence of structurally related molecules	1257:1302	The MIP sensor showed selective molecular recognition for LAC in the presence of structurally related molecules.					
33384130	1	30	theme	dairy	164:168	arg1	products					170:177	dairy products	164:177	dairy products	164:177	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	0	31	theme	imprinted	34:42	arg1	sensor					44:49	Non-enzymatic lactose molecularly imprinted sensor	0:49	Non-enzymatic lactose molecularly imprinted sensor	0:49	Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode.					
33384130	9	32	theme	proposed	1309:1316	arg1	sensor					1325:1330	The proposed PPy/PE sensor	1305:1330	The proposed PPy/PE sensor	1305:1330	The proposed PPy/PE sensor exhibited good stability, as well as excellent reproducibility and repeatability.					
33384130	3	33	theme	LAC	427:429	arg1	detection					414:422	sensitive detection	404:422	sensitive detection of LAC	404:429	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	7	34	theme	linear	1083:1088	arg1	ranges					1090:1095	two dynamic linear ranges	1071:1095	two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1)	1071:1150	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	35	theme	LAC-free	994:1001	arg1	milks					1003:1007	whole and LAC-free milks	984:1007	whole and LAC-free milks	984:1007	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	6	36	theme	sensor	780:785	arg1	performance					787:797	the PPy/PE sensor performance	769:797	the PPy/PE sensor performance	769:797	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	3	37	theme	innovative	359:368	arg1	sensor					393:398	an innovative imprinted voltammetric sensor	356:398	an innovative imprinted voltammetric sensor for sensitive detection of LAC	356:429	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	6	38	theme	PPy/PE	773:778	arg1	performance					787:797	the PPy/PE sensor performance	769:797	the PPy/PE sensor performance	769:797	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	9	39	theme	good	1342:1345	arg1	stability					1347:1355	good stability	1342:1355	good stability	1342:1355	The proposed PPy/PE sensor exhibited good stability, as well as excellent reproducibility and repeatability.					
33384130	3	40	theme	sensor	393:398	arg1	development					341:351	the development	337:351	the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC	337:429	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	3	41	theme	imprinted	370:378	arg1	sensor					393:398	an innovative imprinted voltammetric sensor	356:398	an innovative imprinted voltammetric sensor for sensitive detection of LAC	356:429	The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC.					
33384130	6	42	theme	composite	873:881	arg1	methods					829:835	multivariate methods	816:835	multivariate methods (Plackett-Burman design and central composite design)	816:889	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	42	theme	composite	873:881	arg1	design					883:888	central composite design	865:888	central composite design	865:888	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	7	43	theme	whole	984:988	arg1	milks					1003:1007	whole and LAC-free milks	984:1007	whole and LAC-free milks	984:1007	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	2	44	theme	important	198:206	arg1	indicator					208:216	an important indicator	195:216	an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health	195:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	2	44	theme	important	198:206	arg1	content					184:190	LAC content	180:190	LAC content	180:190	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	8	45	theme	MIP	1196:1198	arg1	sensor					1200:1205	The MIP sensor	1192:1205	The MIP sensor	1192:1205	The MIP sensor showed selective molecular recognition for LAC in the presence of structurally related molecules.					
33384130	0	46	theme	disposable	60:69	arg1	electrode					86:94	disposable graphite paper electrode	60:94	disposable graphite paper electrode	60:94	Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode.					
33384130	4	47	dep	pyrrole	484:490	arg1	MIP					528:530	MIP	528:530	MIP	528:530	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	4	47	dep	pyrrole	484:490	arg1	polymer					519:525	molecularly imprinted polymer	497:525	electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP)	465:531	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	7	48	theme	LAC	967:969	arg1	detection					971:979	LAC detection	967:979	LAC detection in whole and LAC-free milks	967:1007	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	10	49	theme	LAC	1515:1517	arg1	detection					1502:1510	sensitive detection	1492:1510	sensitive detection of LAC	1492:1517	Based on the results obtained, the PPy/PE is found to be highly promising for sensitive detection of LAC.					
33384130	7	50	theme	proposed	924:931	arg1	method					944:949	the proposed analytical method	920:949	the proposed analytical method	920:949	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	2	51	theme	animal	296:301	arg1	health					303:308	human and animal health	286:308	human and animal health	286:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	6	52	theme	several	743:749	arg1	factors					751:757	several factors	743:757	several factors affecting the PPy/PE sensor performance	743:797	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	4	53	theme	imprinted	509:517	arg1	MIP					528:530	MIP	528:530	MIP	528:530	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	4	53	theme	imprinted	509:517	arg1	polymer					519:525	molecularly imprinted polymer	497:525	electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP)	465:531	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	5	54	theme	polypyrrole	628:638	arg1	electrosynthesis					608:623	the electrosynthesis	604:623	the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE)	604:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	5	55	from	PE	692:693	arg1	electrosynthesis					608:623	the electrosynthesis	604:623	the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE)	604:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	1	56	from	products	170:177	arg1	present					144:150	present	144:150	present	144:150	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	7	57	theme	dynamic	1075:1081	arg1	ranges					1090:1095	two dynamic linear ranges	1071:1095	two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1)	1071:1150	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	2	58	theme	LAC	180:182	arg1	indicator					208:216	an important indicator	195:216	an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health	195:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	2	58	theme	LAC	180:182	arg1	content					184:190	LAC content	180:190	LAC content	180:190	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	7	59	theme	analytical	933:942	arg1	method					944:949	the proposed analytical method	920:949	the proposed analytical method	920:949	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	2	60	theme	human	286:290	arg1	health					303:308	human and animal health	286:308	human and animal health	286:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	10	61	theme	sensitive	1492:1500	arg1	detection					1502:1510	sensitive detection	1492:1510	sensitive detection of LAC	1492:1517	Based on the results obtained, the PPy/PE is found to be highly promising for sensitive detection of LAC.					
33384130	9	62	theme	PPy/PE	1318:1323	arg1	sensor					1325:1330	The proposed PPy/PE sensor	1305:1330	The proposed PPy/PE sensor	1305:1330	The proposed PPy/PE sensor exhibited good stability, as well as excellent reproducibility and repeatability.					
33384130	2	63	theme	quality	226:232	arg1	indicator					208:216	an important indicator	195:216	an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health	195:308	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	2	63	theme	quality	226:232	arg1	content					184:190	LAC content	180:190	LAC content	180:190	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	8	64	theme	molecular	1224:1232	arg1	recognition					1234:1244	selective molecular recognition	1214:1244	selective molecular recognition for LAC	1214:1252	The MIP sensor showed selective molecular recognition for LAC in the presence of structurally related molecules.					
33384130	5	65	attach	presence	653:660	arg1	PPy/PE					696:701	PPy/PE	696:701	PPy/PE	696:701	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	5	65	attach	presence	653:660	arg2	molecule					679:686	LAC (template molecule)	665:687	LAC (template molecule)	665:687	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	5	65	attach	presence	653:660	arg1	PE					692:693	PE	692:693	PE (PPy/PE)	692:702	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
33384130	0	66	theme	paper	80:84	arg1	electrode					86:94	disposable graphite paper electrode	60:94	disposable graphite paper electrode	60:94	Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode.					
33384130	2	67	theme	milk	221:224	arg1	quality					226:232	milk quality	221:232	milk quality	221:232	LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health.					
33384130	1	68	theme	major	131:135	arg1	sugar					137:141	major sugar	131:141	major sugar	131:141	Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products.					
33384130	9	69	theme	excellent	1369:1377	arg1	reproducibility					1379:1393	reproducibility	1379:1393	reproducibility	1379:1393	The proposed PPy/PE sensor exhibited good stability, as well as excellent reproducibility and repeatability.					
33384130	0	70	theme	graphite	71:78	arg1	electrode					86:94	disposable graphite paper electrode	60:94	disposable graphite paper electrode	60:94	Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode.					
33384130	4	71	theme	electropolymerized	465:482	arg1	Py					493:494	Py	493:494	Py	493:494	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	4	71	theme	electropolymerized	465:482	arg1	pyrrole					484:490	electropolymerized pyrrole	465:490	electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP)	465:531	The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE).					
33384130	7	72	from	detection	971:979	arg1	milks					1003:1007	whole and LAC-free milks	984:1007	whole and LAC-free milks	984:1007	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	73	theme	optimized	898:906	arg1	conditions					908:917	optimized conditions	898:917	optimized conditions	898:917	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	74	theme	0.88 nmol L-1	1177:1189	arg1	ranges					1090:1095	two dynamic linear ranges	1071:1095	two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1)	1071:1150	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	7	74	theme	0.88 nmol L-1	1177:1189	arg1	limit					1168:1172	a detection limit	1156:1172	a detection limit of 0.88 nmol L-1	1156:1189	Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1.					
33384130	6	75	theme	central	865:871	arg1	methods					829:835	multivariate methods	816:835	multivariate methods (Plackett-Burman design and central composite design)	816:889	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	6	75	theme	central	865:871	arg1	design					883:888	central composite design	865:888	central composite design	865:888	To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design).					
33384130	5	76	theme	LAC	665:667	arg1	molecule					679:686	LAC (template molecule)	665:687	LAC (template molecule)	665:687	The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE).					
34227637	6	0	theme	roasted	1346:1352	arg1	products					1354:1361	roasted products	1346:1361	roasted products	1346:1361	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	6	1	theme	wall	1225:1228	arg1	integrity					1230:1238	cell wall integrity	1220:1238	cell wall integrity	1220:1238	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	3	2	theme	roasting	651:658	arg1	temperature					660:670	roasting temperature	651:670	roasting temperature	651:670	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	2	3	theme	immature	358:365	arg1	rice					367:370	immature rice	358:370	immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading	358:433	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	2	3	theme	immature	358:365	arg1	variety					380:386	TXD306 variety	373:386	TXD306 variety	373:386	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	4	4	theme	increased	995:1003	arg1	riboflavin					1005:1014	slightly increased riboflavin	986:1014	slightly increased riboflavin	986:1014	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	0	5	theme	in	83:84	arg1	starch					92:97	in vitro starch and protein digestibility	83:123	starch	92:97	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	5	6	theme	roasted	1091:1097	arg1	products					1099:1106	roasted products	1091:1106	roasted products	1091:1106	Among roasted products, starch digestibility increased with roasting temperature.					
34227637	6	7	theme	integrity	1230:1238	arg1	loss					1212:1215	a complete loss	1201:1215	a complete loss	1201:1215	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	4	8	theme	nicotinic	1017:1025	arg1	acid					1027:1030	nicotinic acid	1017:1030	nicotinic acid	1017:1030	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	7	9	theme	digestibility	1498:1510	arg1	pepeta					1439:1444	pepeta	1439:1444	pepeta of the highest protein digestibility and low starch digestibility	1439:1510	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	2	10	theme	traditional	297:307	arg1	rice-flakes					309:319	a traditional rice-flakes	295:319	a traditional rice-flakes produced from immature rice grains	295:354	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	6	11	theme	raw	1333:1335	arg1	rice					1337:1340	raw rice	1333:1340	raw rice	1333:1340	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	3	12	theme	temperature	660:670	arg1	severity					639:646	severity	639:646	severity of roasting temperature	639:670	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	4	13	theme	iron	967:970	arg1	content					972:978	significantly increased moisture and iron content	930:978	content	972:978	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	4	14	theme	100	898:900	arg1	°C.					902:904	°C.	902:904	°C.	902:904	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	6	15	theme	cooked	1243:1248	arg1	rice					1250:1253	cooked rice	1243:1253	cooked rice	1243:1253	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	0	16	theme	protein	103:109	arg1	digestibility					111:123	in vitro starch and protein digestibility	83:123	digestibility	111:123	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	0	17	theme	Dry-heat	0:7	arg1	processing					9:18	Dry-heat processing	0:18	Dry-heat processing at different conditions	0:42	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	7	18	theme	optimum	1408:1414	arg1	temperature					1416:1426	the optimum temperature	1404:1426	the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility	1404:1510	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	7	18	theme	optimum	1408:1414	arg1	roasting					1382:1389	roasting	1382:1389	roasting at 100 °C	1382:1399	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	1	19	theme	precious	186:193	arg1	source					207:212	a precious nutritional source	184:212	a precious nutritional source in many rural Africa areas	184:239	Immature grain represents a precious nutritional source in many rural Africa areas.					
34227637	2	20	theme	%	425:425	arg1	heading					427:433	50% heading	423:433	50% heading	423:433	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	0	21	from	conditions	33:42	arg1	processing					9:18	Dry-heat processing	0:18	Dry-heat processing at different conditions	0:42	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	4	22	theme	lipid	798:802	arg1	fiber					781:785	soluble dietary fiber	765:785	soluble dietary fiber	765:785	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	4	22	theme	lipid	798:802	arg1	digestibility					804:816	increased lipid digestibility	788:816	increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products	788:1082	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	6	23	theme	complete	1270:1277	arg1	starch					1279:1284	a complete starch	1268:1284	a complete starch	1268:1284	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	1	24	theme	nutritional	195:205	arg1	source					207:212	a precious nutritional source	184:212	a precious nutritional source in many rural Africa areas	184:239	Immature grain represents a precious nutritional source in many rural Africa areas.					
34227637	2	25	theme	50	423:424	arg1	%					425:425	%	425:425	%	425:425	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	4	26	theme	increased	788:796	arg1	fiber					781:785	soluble dietary fiber	765:785	soluble dietary fiber	765:785	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	4	26	theme	increased	788:796	arg1	digestibility					804:816	increased lipid digestibility	788:816	increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products	788:1082	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	0	27	dep	in	83:84	arg1	vitro					86:90	vitro	86:90	vitro	86:90	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	0	28	theme	different	23:31	arg1	conditions					33:42	different conditions	23:42	different conditions	23:42	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	0	29	theme	immature	128:135	arg1	products					148:155	immature rice-based products	128:155	immature rice-based products	128:155	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	6	30	theme	complete	1203:1210	arg1	loss					1212:1215	a complete loss	1201:1215	a complete loss	1201:1215	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	3	31	contain	has	688:690	arg2	trend					704:708	an opposite trend	692:708	an opposite trend	692:708	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	3	31	contain	has	688:690	arg1	thiamine					679:686	thiamine	679:686	thiamine	679:686	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	4	32	theme	highest	859:865	arg1	value					867:871	the highest value	855:871	the highest value	855:871	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	3	33	theme	nicotinic	572:580	arg1	acid					582:585	nicotinic acid	572:585	nicotinic acid	572:585	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	0	34	dep	composition	67:77	arg1	the					51:53	the	51:53	the	51:53	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	1	35	theme	many	217:220	arg1	areas					235:239	many rural Africa areas	217:239	many rural Africa areas	217:239	Immature grain represents a precious nutritional source in many rural Africa areas.					
34227637	4	36	theme	increased	944:952	arg1	moisture					954:961	significantly increased moisture and iron content	930:978	moisture	954:961	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	0	37	theme	products	148:155	arg1	composition					67:77	nutritional composition	55:77	nutritional composition	55:77	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	0	37	theme	products	148:155	arg1	starch					92:97	in vitro starch and protein digestibility	83:123	starch	92:97	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	0	37	theme	products	148:155	arg1	digestibility					111:123	in vitro starch and protein digestibility	83:123	digestibility	111:123	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	7	38	from	°C	1398:1399	arg1	temperature					1416:1426	the optimum temperature	1404:1426	the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility	1404:1510	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	7	38	from	°C	1398:1399	arg1	roasting					1382:1389	roasting	1382:1389	roasting at 100 °C	1382:1399	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	6	39	theme	cell	1220:1223	arg1	integrity					1230:1238	cell wall integrity	1220:1238	cell wall integrity	1220:1238	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	4	40	theme	unsoaked	1066:1073	arg1	products					1075:1082	unsoaked products	1066:1082	unsoaked products	1066:1082	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	1	41	theme	rural	222:226	arg1	areas					235:239	many rural Africa areas	217:239	many rural Africa areas	217:239	Immature grain represents a precious nutritional source in many rural Africa areas.					
34227637	0	42	theme	rice-based	137:146	arg1	products					148:155	immature rice-based products	128:155	immature rice-based products	128:155	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	7	43	theme	low	1487:1489	arg1	digestibility					1498:1510	low starch digestibility	1487:1510	low starch digestibility	1487:1510	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	4	44	theme	insoluble	750:758	arg1	transformation					732:745	the transformation	728:745	the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products	728:1082	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	1	45	theme	Africa	228:233	arg1	areas					235:239	many rural Africa areas	217:239	many rural Africa areas	217:239	Immature grain represents a precious nutritional source in many rural Africa areas.					
34227637	0	46	theme	nutritional	55:65	arg1	composition					67:77	nutritional composition	55:77	nutritional composition	55:77	Dry-heat processing at different conditions impact the nutritional composition and in vitro starch and protein digestibility of immature rice-based products.					
34227637	5	47	theme	starch	1109:1114	arg1	digestibility					1116:1128	starch digestibility	1109:1128	starch digestibility	1109:1128	Among roasted products, starch digestibility increased with roasting temperature.					
34227637	6	48	theme	protein	1290:1296	arg1	digestion					1298:1306	protein digestion	1290:1306	protein digestion	1290:1306	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	7	49	theme	starch	1491:1496	arg1	digestibility					1498:1510	low starch digestibility	1487:1510	low starch digestibility	1487:1510	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	4	50	theme	dietary	773:779	arg1	fiber					781:785	soluble dietary fiber	765:785	soluble dietary fiber	765:785	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	4	50	theme	dietary	773:779	arg1	digestibility					804:816	increased lipid digestibility	788:816	increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products	788:1082	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	3	51	theme	opposite	695:702	arg1	trend					704:708	an opposite trend	692:708	an opposite trend	692:708	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	7	52	theme	digestibility	1469:1481	arg1	pepeta					1439:1444	pepeta	1439:1444	pepeta of the highest protein digestibility and low starch digestibility	1439:1510	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	4	53	theme	soluble	765:771	arg1	fiber					781:785	soluble dietary fiber	765:785	soluble dietary fiber	765:785	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	4	53	theme	soluble	765:771	arg1	digestibility					804:816	increased lipid digestibility	788:816	increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products	788:1082	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	6	54	from	loss	1212:1215	arg1	rice					1250:1253	cooked rice	1243:1253	cooked rice	1243:1253	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	2	55	theme	temperature	518:528	arg1	regimes					551:557	different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes	474:557	regimes	551:557	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	2	55	theme	temperature	518:528	arg1	°C					547:548	80, 100 and 120 °C	531:548	80, 100 and 120 °C	531:548	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	3	56	theme	iron	605:608	arg1	concentration					610:622	iron concentration	605:622	iron concentration	605:622	Riboflavin, nicotinic acid, nicotinamide and iron concentration increased with severity of roasting temperature, while thiamine has an opposite trend.					
34227637	2	57	theme	rice	277:280	arg1	processing					254:263	processing	254:263	processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains)	254:355	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	8	58	from	°C	1544:1545	arg1	Soaking					1513:1519	Soaking	1513:1519	Soaking before roasting at 120 °C	1513:1545	Soaking before roasting at 120 °C is best when retaining micronutrients is considered.					
34227637	2	59	theme	rice	344:347	arg1	grains					349:354	immature rice grains	335:354	immature rice grains	335:354	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	2	60	theme	roasting	509:516	arg1	regimes					551:557	different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes	474:557	regimes	551:557	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	2	60	theme	roasting	509:516	arg1	°C					547:548	80, 100 and 120 °C	531:548	80, 100 and 120 °C	531:548	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	7	61	theme	highest	1453:1459	arg1	digestibility					1469:1481	the highest protein digestibility	1449:1481	the highest protein digestibility	1449:1481	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	2	62	theme	immature	268:275	arg1	rice					277:280	immature rice	268:280	immature rice	268:280	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	2	63	theme	immature	335:342	arg1	grains					349:354	immature rice grains	335:354	immature rice grains	335:354	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	7	64	theme	protein	1461:1467	arg1	digestibility					1469:1481	the highest protein digestibility	1449:1481	the highest protein digestibility	1449:1481	We concluded that roasting at 100 °C is the optimum temperature to produce pepeta of the highest protein digestibility and low starch digestibility.					
34227637	1	65	from	source	207:212	arg1	areas					235:239	many rural Africa areas	217:239	many rural Africa areas	217:239	Immature grain represents a precious nutritional source in many rural Africa areas.					
34227637	2	66	dep	pepeta	287:292	arg1	rice-flakes					309:319	a traditional rice-flakes	295:319	a traditional rice-flakes produced from immature rice grains	295:354	To optimize processing of immature rice into pepeta (a traditional rice-flakes produced from immature rice grains), immature rice (TXD306 variety) harvested at 18 and 26 days after 50% heading were processed in the laboratory under different soaking (0 and 12 h) and roasting temperature (80, 100 and 120 °C) regimes.					
34227637	4	67	from	Soaking	906:912	arg1	roasted					887:893	roasted	887:893	roasted	887:893	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	4	68	theme	°C.	902:904	arg1	Soaking					906:912	100 °C. Soaking	898:912	100 °C. Soaking	898:912	Heating promoted the transformation of insoluble into soluble dietary fiber, increased lipid digestibility decreasing protein one, which showed the highest value when rice was roasted at 100 °C. Soaking before roasting significantly increased moisture and iron content while slightly increased riboflavin, nicotinic acid and nicotinamide when compared to unsoaked products.					
34227637	6	69	theme	Microstructure	1167:1180	arg1	analysis					1182:1189	Microstructure analysis	1167:1189	Microstructure analysis	1167:1189	Microstructure analysis indicated a complete loss of cell wall integrity in cooked rice, determining a complete starch and protein digestion while this is delayed in raw rice and roasted products.					
34227637	1	70	theme	Immature	158:165	arg1	grain					167:171	Immature grain	158:171	Immature grain	158:171	Immature grain represents a precious nutritional source in many rural Africa areas.					
33454617	0	0	theme	extender	77:84	arg1	characteristics					93:107	bull semen extender: sperm characteristics post-thawing	66:120	bull semen extender: sperm characteristics post-thawing	66:120	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	8	1	theme	functions	1549:1557	arg1	timing					1531:1536	timing	1531:1536	timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI)	1531:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	0	2	dep	in	126:127	arg1	vivo					129:132	vivo	129:132	vivo	129:132	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	6	3	theme	computer-assisted	973:989	arg1	analysis					997:1004	the computer-assisted sperm analysis	969:1004	the computer-assisted sperm analysis	969:1004	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	0	4	theme	semen	71:75	arg1	characteristics					93:107	bull semen extender: sperm characteristics post-thawing	66:120	bull semen extender: sperm characteristics post-thawing	66:120	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	8	5	theme	treatment	1496:1504	arg1	regimen					1506:1512	a hormonal treatment regimen	1485:1512	a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI)	1485:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	9	6	theme	cholesterol-cyclodextrin	1645:1668	arg1	extender					1684:1691	cholesterol-cyclodextrin Tris-glycerol extender	1645:1691	cholesterol-cyclodextrin Tris-glycerol extender	1645:1691	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	3	7	theme	plasma	477:482	arg1	membrane					484:491	the sperm plasma membrane	467:491	the sperm plasma membrane	467:491	Cholesterol may be delivered and incorporated into the sperm plasma membrane by cyclodextrins to protect sperm during cryopreservation.					
33454617	0	8	theme	sperm	87:91	arg1	characteristics					93:107	bull semen extender: sperm characteristics post-thawing	66:120	bull semen extender: sperm characteristics post-thawing	66:120	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	8	9	theme	hormonal	1487:1494	arg1	regimen					1506:1512	a hormonal treatment regimen	1485:1512	a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI)	1485:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	9	10	theme	Tris-glycerol	1670:1682	arg1	extender					1684:1691	cholesterol-cyclodextrin Tris-glycerol extender	1645:1691	cholesterol-cyclodextrin Tris-glycerol extender	1645:1691	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	4	11	theme	study	568:572	arg1	aim					556:558	The aim	552:558	The aim of this study	552:572	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	9	12	theme	cryopreserve	1782:1793	arg1	sperm					1800:1804	cryopreserve bull sperm	1782:1804	cryopreserve bull sperm	1782:1804	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	0	13	from	yolk	58:61	arg1	fertility					134:142	in vivo fertility	126:142	in vivo fertility	126:142	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	0	13	from	yolk	58:61	arg1	characteristics					93:107	bull semen extender: sperm characteristics post-thawing	66:120	bull semen extender: sperm characteristics post-thawing	66:120	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	8	14	theme	pregnancy	1304:1312	arg1	rate					1314:1317	a pregnancy rate	1302:1317	a pregnancy rate of as great as 56 %	1302:1337	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	7	15	theme	TG	1233:1234	arg1	extender					1236:1243	TG extender	1233:1243	TG extender	1233:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	3	16	theme	sperm	471:475	arg1	membrane					484:491	the sperm plasma membrane	467:491	the sperm plasma membrane	467:491	Cholesterol may be delivered and incorporated into the sperm plasma membrane by cyclodextrins to protect sperm during cryopreservation.					
33454617	5	17	theme	Tris-glycerol	896:908	arg1	extender					915:922	Tris-glycerol (TG) extender	896:922	Tris-glycerol (TG) extender	896:922	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	0	18	theme	post-thawing	109:120	arg1	characteristics					93:107	bull semen extender: sperm characteristics post-thawing	66:120	bull semen extender: sperm characteristics post-thawing	66:120	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	5	19	theme	different	806:814	arg1	concentrations					816:829	three different concentrations	800:829	three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen)	800:891	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	5	20	theme	complex	859:865	arg1	concentrations					816:829	three different concentrations	800:829	three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen)	800:891	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	9	21	theme	acceptable	1823:1832	arg1	rates					1844:1848	acceptable pregnancy rates	1823:1848	acceptable pregnancy rates	1823:1848	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	5	22	theme	cholesterol-cyclodextrin	834:857	arg1	complex					859:865	cholesterol-cyclodextrin complex	834:865	cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen)	834:891	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	5	22	theme	cholesterol-cyclodextrin	834:857	arg1	semen					886:890	0.5, 1 or 2 mg/mL semen	868:890	0.5, 1 or 2 mg/mL semen	868:890	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	9	23	used	used	1774:1777	arg2	extender					1684:1691	cholesterol-cyclodextrin Tris-glycerol extender	1645:1691	cholesterol-cyclodextrin Tris-glycerol extender	1645:1691	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	8	24	theme	fixed-time	1585:1594	arg1	insemination					1607:1618	fixed-time artificial insemination	1585:1618	fixed-time artificial insemination (FTAI)	1585:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	8	24	theme	fixed-time	1585:1594	arg1	FTAI					1621:1624	FTAI	1621:1624	FTAI	1621:1624	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	2	25	theme	semen	358:362	arg1	extender					364:371	the semen extender	354:371	the semen extender	354:371	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	2	26	from	inclusion	323:331	arg1	extender					364:371	the semen extender	354:371	the semen extender	354:371	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	8	27	theme	artificial	1596:1605	arg1	insemination					1607:1618	fixed-time artificial insemination	1585:1618	fixed-time artificial insemination (FTAI)	1585:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	8	27	theme	artificial	1596:1605	arg1	FTAI					1621:1624	FTAI	1621:1624	FTAI	1621:1624	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	7	28	theme	lesser	1257:1262	arg1	P <					1281:1283	P <	1281:1283	P <	1281:1283	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	7	28	theme	lesser	1257:1262	arg1	rates					1274:1278	lesser pregnancy rates	1257:1278	lesser pregnancy rates (P < 0.05)	1257:1289	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	1	29	theme	semen	163:167	arg1	yolk					149:152	Egg yolk	145:152	Egg yolk	145:152	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	1	29	theme	semen	163:167	arg1	constituent					178:188	a major semen extender constituent	155:188	a major semen extender constituent	155:188	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	7	30	theme	cholesterol-cyclodextrin	1160:1183	arg1	complex					1185:1191	cholesterol-cyclodextrin complex	1160:1191	cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender	1160:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	7	30	theme	cholesterol-cyclodextrin	1160:1183	arg1	semen					1202:1206	2 mg/mL semen	1194:1206	2 mg/mL semen	1194:1206	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	8	31	used	used	1430:1433	arg2	sperm					1344:1348	sperm	1344:1348	sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1344:1423	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	7	32	from	dilution	1221:1228	arg1	extender					1236:1243	TG extender	1233:1243	TG extender	1233:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	4	33	theme	semen	709:713	arg1	cryopreservation					684:699	cryopreservation	684:699	cryopreservation of bull semen	684:713	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	1	34	theme	extender	169:176	arg1	yolk					149:152	Egg yolk	145:152	Egg yolk	145:152	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	1	34	theme	extender	169:176	arg1	constituent					178:188	a major semen extender constituent	155:188	a major semen extender constituent	155:188	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	2	35	theme	important	391:399	arg1	consideration					401:413	an important consideration	388:413	an important consideration	388:413	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	2	35	theme	important	391:399	arg1	Cryopreservation					284:299	Cryopreservation	284:299	Cryopreservation of bull semen without inclusion of animal protein in the semen extender	284:371	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	6	36	theme	motion	931:936	arg1	characteristics					938:952	Sperm motion characteristics	925:952	Sperm motion characteristics examined using the computer-assisted sperm analysis	925:1004	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	0	37	theme	in	126:127	arg1	fertility					134:142	in vivo fertility	126:142	in vivo fertility	126:142	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	8	38	theme	%	1337:1337	arg1	rate					1314:1317	a pregnancy rate	1302:1317	a pregnancy rate of as great as 56 %	1302:1337	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	6	39	theme	acrosome	1031:1038	arg1	integrity					1040:1048	acrosome integrity	1031:1048	acrosome integrity	1031:1048	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	5	40	theme	TG	911:912	arg1	extender					915:922	Tris-glycerol (TG) extender	896:922	Tris-glycerol (TG) extender	896:922	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	6	41	theme	Sperm	925:929	arg1	characteristics					938:952	Sperm motion characteristics	925:952	Sperm motion characteristics examined using the computer-assisted sperm analysis	925:1004	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	4	42	theme	yolk	674:677	arg1	inclusion					657:665	inclusion	657:665	inclusion of egg yolk	657:677	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	4	43	theme	extender	639:646	arg1	suitability					591:601	suitability	591:601	suitability of a cholesterol-cyclodextrin semen extender	591:646	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	4	44	theme	egg	670:672	arg1	yolk					674:677	egg yolk	670:677	egg yolk	670:677	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	7	45	theme	2 mg/mL	1194:1200	arg1	complex					1185:1191	cholesterol-cyclodextrin complex	1160:1191	cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender	1160:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	7	45	theme	2 mg/mL	1194:1200	arg1	semen					1202:1206	2 mg/mL semen	1194:1206	2 mg/mL semen	1194:1206	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	8	46	theme	ovarian	1541:1547	arg1	functions					1549:1557	ovarian functions	1541:1557	ovarian functions among cows for conducting fixed-time artificial insemination (FTAI)	1541:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	5	47	theme	concentrations	816:829	arg1	inclusions					786:795	inclusions	786:795	inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen)	786:891	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	4	48	theme	semen	633:637	arg1	extender					639:646	a cholesterol-cyclodextrin semen extender	606:646	a cholesterol-cyclodextrin semen extender	606:646	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	6	49	theme	flow	1065:1068	arg1	cytometry					1070:1078	flow cytometry	1065:1078	flow cytometry	1065:1078	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	1	50	theme	egg	274:276	arg1	yolk					278:281	egg yolk	274:281	egg yolk	274:281	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	9	51	used	used	1869:1872	arg2	semen					1860:1864	this semen	1855:1864	this semen	1855:1864	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	7	52	theme	greatest	1134:1141	arg1	concentration					1143:1155	the greatest concentration	1130:1155	the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender	1130:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	9	53	theme	bull	1795:1798	arg1	sperm					1800:1804	cryopreserve bull sperm	1782:1804	cryopreserve bull sperm	1782:1804	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	4	54	theme	cholesterol-cyclodextrin	608:631	arg1	extender					639:646	a cholesterol-cyclodextrin semen extender	606:646	a cholesterol-cyclodextrin semen extender	606:646	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	5	55	theme	egg	769:771	arg1	yolk					773:776	either egg yolk	762:776	either egg yolk	762:776	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
33454617	1	56	theme	yolk	278:281	arg1	use					267:269	use	267:269	use of egg yolk	267:281	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	8	57	theme	timing	1531:1536	arg1	synchrony					1518:1526	synchrony	1518:1526	synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI)	1518:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	9	58	with	extender	1684:1691	arg1	composition					1720:1730	a chemically defined composition	1699:1730	a chemically defined composition	1699:1730	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	7	59	theme	pregnancy	1264:1272	arg1	P <					1281:1283	P <	1281:1283	P <	1281:1283	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	7	59	theme	pregnancy	1264:1272	arg1	rates					1274:1278	lesser pregnancy rates	1257:1278	lesser pregnancy rates (P < 0.05)	1257:1289	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	0	60	theme	egg	54:56	arg1	yolk					58:61	egg yolk	54:61	egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility	54:142	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	9	61	theme	defined	1712:1718	arg1	composition					1720:1730	a chemically defined composition	1699:1730	a chemically defined composition	1699:1730	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	7	62	theme	concentration	1143:1155	arg1	inclusion					1117:1125	The inclusion	1113:1125	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender	1113:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	2	63	theme	semen	309:313	arg1	consideration					401:413	an important consideration	388:413	an important consideration	388:413	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	2	63	theme	semen	309:313	arg1	Cryopreservation					284:299	Cryopreservation	284:299	Cryopreservation of bull semen without inclusion of animal protein in the semen extender	284:371	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	8	64	theme	cholesterol-cyclodextrin	1377:1400	arg1	extender					1416:1423	0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1367:1423	0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1367:1423	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	9	65	theme	egg	1757:1759	arg1	yolk					1761:1764	egg yolk	1757:1764	egg yolk	1757:1764	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	8	66	theme	artificial	1439:1448	arg1	insemination					1450:1461	artificial insemination	1439:1461	artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI)	1439:1625	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	8	67	theme	mg/mL	1371:1375	arg1	extender					1416:1423	0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1367:1423	0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1367:1423	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	2	68	theme	bull	304:307	arg1	semen					309:313	bull semen	304:313	bull semen	304:313	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	1	69	theme	defined	199:205	arg1	composition					207:217	a defined composition	197:217	a defined composition	197:217	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	9	70	theme	yolk	1761:1764	arg1	inclusion					1744:1752	inclusion	1744:1752	inclusion of egg yolk	1744:1764	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	8	71	theme	0.5	1367:1369	arg1	mg/mL					1371:1375	mg/mL	1371:1375	mg/mL	1371:1375	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	1	72	theme	Egg	145:147	arg1	yolk					149:152	Egg yolk	145:152	Egg yolk	145:152	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	1	72	theme	Egg	145:147	arg1	constituent					178:188	a major semen extender constituent	155:188	a major semen extender constituent	155:188	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	9	73	theme	pregnancy	1834:1842	arg1	rates					1844:1848	acceptable pregnancy rates	1823:1848	acceptable pregnancy rates	1823:1848	Results indicate cholesterol-cyclodextrin Tris-glycerol extender, with a chemically defined composition and without inclusion of egg yolk, may be used to cryopreserve bull sperm with there being acceptable pregnancy rates when this semen is used for FTAI.					
33454617	4	74	theme	bull	704:707	arg1	semen					709:713	bull semen	704:713	bull semen	704:713	The aim of this study was to determine suitability of a cholesterol-cyclodextrin semen extender, without inclusion of egg yolk, for cryopreservation of bull semen.					
33454617	0	75	theme	bull	66:69	arg1	characteristics					93:107	bull semen extender: sperm characteristics post-thawing	66:120	bull semen extender: sperm characteristics post-thawing	66:120	Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility.					
33454617	6	76	theme	plasma	1011:1016	arg1	membrane					1018:1025	plasma membrane	1011:1025	plasma membrane	1011:1025	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	8	77	theme	Tris-glycerol	1402:1414	arg1	extender					1416:1423	0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1367:1423	0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender	1367:1423	There was a pregnancy rate of as great as 56 % when sperm cryopreserved in 0.5 mg/mL cholesterol-cyclodextrin Tris-glycerol extender were used for artificial insemination following imposing of a hormonal treatment regimen for synchrony of timing of ovarian functions among cows for conducting fixed-time artificial insemination (FTAI).					
33454617	2	78	theme	protein	343:349	arg1	inclusion					323:331	inclusion	323:331	inclusion of animal protein in the semen extender	323:371	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	1	79	theme	major	157:161	arg1	yolk					149:152	Egg yolk	145:152	Egg yolk	145:152	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	1	79	theme	major	157:161	arg1	constituent					178:188	a major semen extender constituent	155:188	a major semen extender constituent	155:188	Egg yolk, a major semen extender constituent, lacks a defined composition, therefore, there are biosecurity concerns with use of egg yolk.					
33454617	7	80	theme	complex	1185:1191	arg1	concentration					1143:1155	the greatest concentration	1130:1155	the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender	1130:1243	The inclusion of the greatest concentration of cholesterol-cyclodextrin complex (2 mg/mL semen) followed by dilution in TG extender resulted in lesser pregnancy rates (P < 0.05).					
33454617	6	81	theme	sperm	991:995	arg1	analysis					997:1004	the computer-assisted sperm analysis	969:1004	the computer-assisted sperm analysis	969:1004	Sperm motion characteristics examined using the computer-assisted sperm analysis, and plasma membrane and acrosome integrity examined using flow cytometry, were similar for all extenders.					
33454617	2	82	theme	animal	336:341	arg1	protein					343:349	animal protein	336:349	animal protein	336:349	Cryopreservation of bull semen without inclusion of animal protein in the semen extender, therefore, is an important consideration.					
33454617	5	83	theme	Bull	716:719	arg1	semen					721:725	Bull semen	716:725	Bull semen	716:725	Bull semen was collected and cryopreserved in either egg yolk or with inclusions of three different concentrations of cholesterol-cyclodextrin complex (0.5, 1 or 2 mg/mL semen) in Tris-glycerol (TG) extender.					
34606538	3	0	theme	HFD-fed	827:833	arg1	mice					835:838	the HFD-fed mice	823:838	the HFD-fed mice	823:838	Furthermore, the health of the intestinal microbiota in the HFD-fed mice was altered dramatically after DCSK consumption.					
34606538	2	1	theme	physiological	507:519	arg1	suppression					705:715	the suppression	701:715	the suppression of the oxidative stress of the liver and kidneys	701:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	1	theme	physiological	507:519	arg1	indices					521:527	physiological indices	507:527	physiological indices	507:527	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	1	theme	physiological	507:519	arg1	decrease					581:588	the decrease	577:588	the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels	577:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	3	2	theme	microbiota	809:818	arg1	health					784:789	the health	780:789	the health of the intestinal microbiota in the HFD-fed mice	780:838	Furthermore, the health of the intestinal microbiota in the HFD-fed mice was altered dramatically after DCSK consumption.					
34606538	1	3	theme	DCSK	265:268	arg1	composite					271:279	a debranched corn starch/konjac glucomannan (DCSK) composite	220:279	a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	220:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	0	4	theme	gut	107:109	arg1	microbiota					111:120	gut microbiota	107:120	gut microbiota	107:120	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	1	5	theme	gut	339:341	arg1	microbiota					343:352	gut microbiota	339:352	gut microbiota	339:352	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	2	6	theme	HFD	556:558	arg1	group					560:564	the HFD group	552:564	the HFD group	552:564	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	5	7	contain	has	1118:1120	arg1	DCSK					1113:1116	DCSK	1113:1116	DCSK	1113:1116	These findings demonstrated that DCSK has an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D.					
34606538	5	7	contain	has	1118:1120	arg2	ability					1137:1143	an outstanding ability	1122:1143	an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D	1122:1224	These findings demonstrated that DCSK has an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D.					
34606538	2	8	dep	parameters	492:501	arg1	the					476:478	the	476:478	the	476:478	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	9	from	T2D	545:547	arg1	group					560:564	the HFD group	552:564	the HFD group	552:564	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	1	10	theme	composite	271:279	arg1	mechanism					207:215	the protection mechanism	192:215	the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	192:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	4	11	theme	differential	914:925	arg1	metabolites					927:937	13 differential metabolites	911:937	13 differential metabolites strongly linked to DCSK intervention	911:974	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	4	12	attach	linked	948:953	arg2	metabolites					927:937	13 differential metabolites	911:937	13 differential metabolites strongly linked to DCSK intervention	911:974	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	4	12	attach	linked	948:953	arg1	intervention					963:974	DCSK intervention	958:974	DCSK intervention	958:974	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	2	13	theme	blood	593:597	arg1	glucose					599:605	blood glucose	593:605	blood glucose	593:605	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	4	14	theme	lipid	1062:1066	arg1	metabolism					1068:1077	lipid metabolism	1062:1077	lipid metabolism	1062:1077	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	1	15	from	dyslipidemia	322:333	arg1	mice					357:360	mice	357:360	mice fed on a high-fat diet (HFD)	357:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	2	16	theme	DCSK	435:438	arg1	consumption					420:430	the consumption	416:430	the consumption of DCSK	416:438	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	17	dep	liver	748:752	arg1	the					744:746	the	744:746	the	744:746	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	0	18	theme	protective	4:13	arg1	mechanism					15:23	The protective mechanism	0:23	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.	0:162	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	2	19	theme	significant	449:459	arg1	improvement					461:471	a significant improvement	447:471	a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys	447:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	3	20	from	health	784:789	arg1	mice					835:838	the HFD-fed mice	823:838	the HFD-fed mice	823:838	Furthermore, the health of the intestinal microbiota in the HFD-fed mice was altered dramatically after DCSK consumption.					
34606538	4	21	theme	DCSK	981:984	arg1	supplementation					986:1000	DCSK supplementation	981:1000	DCSK supplementation	981:1000	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	2	22	theme	total	622:626	arg1	cholesterol					628:638	total cholesterol	622:638	total cholesterol	622:638	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	1	23	from	microbiota	343:352	arg1	mice					357:360	mice	357:360	mice fed on a high-fat diet (HFD)	357:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	1	24	theme	type	289:292	arg1	T2D					306:308	T2D	306:308	T2D	306:308	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	1	24	theme	type	289:292	arg1	diabetes					296:303	type 2 diabetes	289:303	type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	289:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	0	25	theme	induced	139:145	arg1	diabetes					154:161	high-fat-diet induced type 2 diabetes	125:161	high-fat-diet induced type 2 diabetes	125:161	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	2	26	from	suppression	705:715	arg1	triglyceride					608:619	triglyceride	608:619	triglyceride	608:619	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	26	from	suppression	705:715	arg1	cholesterol					628:638	total cholesterol	622:638	total cholesterol	622:638	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	26	from	suppression	705:715	arg1	glucose					599:605	blood glucose	593:605	blood glucose	593:605	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	26	from	suppression	705:715	arg1	levels					682:687	high-density lipoprotein cholesterol levels	645:687	high-density lipoprotein cholesterol levels	645:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	27	theme	stress	734:739	arg1	suppression					705:715	the suppression	701:715	the suppression of the oxidative stress of the liver and kidneys	701:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	27	theme	stress	734:739	arg1	decrease					581:588	the decrease	577:588	the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels	577:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	1	28	theme	protection	196:205	arg1	mechanism					207:215	the protection mechanism	192:215	the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	192:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	0	29	theme	high-fat-diet	125:137	arg1	diabetes					154:161	high-fat-diet induced type 2 diabetes	125:161	high-fat-diet induced type 2 diabetes	125:161	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	0	30	from	dyslipidemia	90:101	arg1	diabetes					154:161	high-fat-diet induced type 2 diabetes	125:161	high-fat-diet induced type 2 diabetes	125:161	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	2	31	theme	oxidative	724:732	arg1	stress					734:739	the oxidative stress	720:739	the oxidative stress of the liver and kidneys	720:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	0	32	theme	debranched	30:39	arg1	composite					72:80	a debranched corn starch/konjac glucomannan composite	28:80	a debranched corn starch/konjac glucomannan composite	28:80	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	4	33	theme	nucleotide	1035:1044	arg1	metabolism					1046:1055	nucleotide metabolism	1035:1055	nucleotide metabolism	1035:1055	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	3	34	theme	DCSK	871:874	arg1	consumption					876:886	DCSK consumption	871:886	DCSK consumption	871:886	Furthermore, the health of the intestinal microbiota in the HFD-fed mice was altered dramatically after DCSK consumption.					
34606538	4	35	theme	DCSK	958:961	arg1	intervention					963:974	DCSK intervention	958:974	DCSK intervention	958:974	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	0	36	theme	type	147:150	arg1	diabetes					154:161	high-fat-diet induced type 2 diabetes	125:161	high-fat-diet induced type 2 diabetes	125:161	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	1	37	theme	high-fat	371:378	arg1	HFD					386:388	HFD	386:388	HFD	386:388	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	1	37	theme	high-fat	371:378	arg1	diet					380:383	a high-fat diet	369:383	a high-fat diet (HFD)	369:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	4	38	theme	amino	1012:1016	arg1	metabolism					1023:1032	amino acid metabolism	1012:1032	amino acid metabolism	1012:1032	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	2	39	theme	cholesterol	670:680	arg1	levels					682:687	high-density lipoprotein cholesterol levels	645:687	high-density lipoprotein cholesterol levels	645:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	40	from	decrease	581:588	arg1	triglyceride					608:619	triglyceride	608:619	triglyceride	608:619	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	40	from	decrease	581:588	arg1	cholesterol					628:638	total cholesterol	622:638	total cholesterol	622:638	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	40	from	decrease	581:588	arg1	glucose					599:605	blood glucose	593:605	blood glucose	593:605	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	40	from	decrease	581:588	arg1	levels					682:687	high-density lipoprotein cholesterol levels	645:687	high-density lipoprotein cholesterol levels	645:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	0	41	theme	starch/konjac	46:58	arg1	composite					72:80	a debranched corn starch/konjac glucomannan composite	28:80	a debranched corn starch/konjac glucomannan composite	28:80	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	2	42	theme	biochemical	480:490	arg1	suppression					705:715	the suppression	701:715	the suppression of the oxidative stress of the liver and kidneys	701:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	42	theme	biochemical	480:490	arg1	parameters					492:501	biochemical parameters	480:501	biochemical parameters	480:501	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	42	theme	biochemical	480:490	arg1	decrease					581:588	the decrease	577:588	the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels	577:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	43	theme	lipoprotein	658:668	arg1	levels					682:687	high-density lipoprotein cholesterol levels	645:687	high-density lipoprotein cholesterol levels	645:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	0	44	theme	corn	41:44	arg1	composite					72:80	a debranched corn starch/konjac glucomannan composite	28:80	a debranched corn starch/konjac glucomannan composite	28:80	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	2	45	from	improvement	461:471	arg1	suppression					705:715	the suppression	701:715	the suppression of the oxidative stress of the liver and kidneys	701:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	45	from	improvement	461:471	arg1	parameters					492:501	biochemical parameters	480:501	biochemical parameters	480:501	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	45	from	improvement	461:471	arg1	indices					521:527	physiological indices	507:527	physiological indices	507:527	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	45	from	improvement	461:471	arg1	decrease					581:588	the decrease	577:588	the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels	577:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	46	theme	high-density	645:656	arg1	levels					682:687	high-density lipoprotein cholesterol levels	645:687	high-density lipoprotein cholesterol levels	645:687	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	2	47	theme	kidneys	758:764	arg1	stress					734:739	the oxidative stress	720:739	the oxidative stress of the liver and kidneys	720:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	1	48	theme	related	311:317	arg1	T2D					306:308	T2D	306:308	T2D	306:308	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	1	48	theme	related	311:317	arg1	diabetes					296:303	type 2 diabetes	289:303	type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	289:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	0	49	from	microbiota	111:120	arg1	diabetes					154:161	high-fat-diet induced type 2 diabetes	125:161	high-fat-diet induced type 2 diabetes	125:161	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	3	50	theme	intestinal	798:807	arg1	microbiota					809:818	the intestinal microbiota	794:818	the intestinal microbiota	794:818	Furthermore, the health of the intestinal microbiota in the HFD-fed mice was altered dramatically after DCSK consumption.					
34606538	1	51	theme	debranched	222:231	arg1	composite					271:279	a debranched corn starch/konjac glucomannan (DCSK) composite	220:279	a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	220:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	5	52	dep	hyperglycemia	1156:1168	arg1	microbiota					1195:1204	microbiota	1195:1204	microbiota	1195:1204	These findings demonstrated that DCSK has an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D.					
34606538	4	53	theme	acid	1018:1021	arg1	metabolism					1023:1032	amino acid metabolism	1012:1032	amino acid metabolism	1012:1032	Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism.					
34606538	2	54	theme	liver	748:752	arg1	stress					734:739	the oxidative stress	720:739	the oxidative stress of the liver and kidneys	720:764	The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys.					
34606538	1	55	theme	corn	233:236	arg1	composite					271:279	a debranched corn starch/konjac glucomannan (DCSK) composite	220:279	a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	220:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	0	56	theme	composite	72:80	arg1	mechanism					15:23	The protective mechanism	0:23	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.	0:162	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	1	57	theme	starch/konjac	238:250	arg1	composite					271:279	a debranched corn starch/konjac glucomannan (DCSK) composite	220:279	a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	220:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34606538	0	58	theme	glucomannan	60:70	arg1	composite					72:80	a debranched corn starch/konjac glucomannan composite	28:80	a debranched corn starch/konjac glucomannan composite	28:80	The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.					
34606538	5	59	theme	outstanding	1125:1135	arg1	ability					1137:1143	an outstanding ability	1122:1143	an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D	1122:1224	These findings demonstrated that DCSK has an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D.					
34606538	1	60	theme	glucomannan	252:262	arg1	composite					271:279	a debranched corn starch/konjac glucomannan (DCSK) composite	220:279	a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD)	220:389	This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD).					
34364648	16	0	theme	sdLys	2332:2336	arg1	supply					2338:2343	sdLys supply	2332:2343	sdLys supply	2332:2343	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	15	1	theme	DM	2207:2208	arg1	intake					2210:2215	high dietary FA, decreased DM intake	2180:2215	intake	2210:2215	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	1	2	theme	Lys	227:229	arg1	effects					138:144	The effects	134:144	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization	134:290	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	4	3	theme	±	702:702	arg1	SD					704:705	mean ± SD	697:705	mean ± SD; 80 ± 14 d in milk	697:724	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	7	4	theme	protein	1166:1172	arg1	yield					1174:1178	milk protein yield	1161:1178	milk protein yield	1161:1178	Increasing dietary FA decreased DM intake and milk protein yield.					
34364648	14	5	theme	N	2074:2074	arg1	balance					2076:2082	N balance	2074:2082	N balance	2074:2082	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	5	6	theme	total	948:952	arg1	collection					954:963	total collection	948:963	total collection	948:963	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	12	7	theme	dietary	1729:1735	arg1	FA					1737:1738	dietary FA	1729:1738	dietary FA	1729:1738	Supply of ME increased as dietary FA increased from 3.0 to 4.2% and decreased as FA increased beyond 4.2%.					
34364648	4	8	from	blocks	736:741	arg1	design					784:789	a partially balanced incomplete block design	746:789	a partially balanced incomplete block design	746:789	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	1	9	theme	acid	163:166	arg1	effects					138:144	The effects	134:144	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization	134:290	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	4	10	theme	Jersey	684:689	arg1	cows					691:694	25 Jersey cows	681:694	25 Jersey cows (mean ± SD; 80 ± 14 d in milk)	681:725	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	7	11	theme	DM	1147:1148	arg1	intake					1150:1155	dietary FA decreased DM intake	1126:1155	dietary FA decreased DM intake	1126:1155	Increasing dietary FA decreased DM intake and milk protein yield.					
34364648	15	12	theme	ME	2263:2264	arg1	content					2266:2272	ME content	2263:2272	ME content	2263:2272	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	16	13	theme	protein	2454:2460	arg1	synthesis					2462:2470	muscle protein synthesis	2447:2470	muscle protein synthesis at the expense of milk protein	2447:2501	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	9	14	theme	dietary	1420:1426	arg1	FA					1428:1429	dietary FA	1420:1429	dietary FA	1420:1429	Digestibility of FA increased when dietary FA increased from 3.0 to 4.2% and decreased as FA increased beyond 4.2%.					
34364648	3	15	theme	corn	571:574	arg1	grain					576:580	corn grain	571:580	corn grain	571:580	Dietary FA and starch were increased by replacing soyhulls with supplemental fat and corn grain, respectively, and sdLys increased with rumen-protected Lys.					
34364648	0	16	theme	lactating	111:119	arg1	cows					128:131	lactating Jersey cows	111:131	lactating Jersey cows	111:131	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	5	17	theme	d	832:832	arg1	periods					818:824	4 periods	816:824	4 periods of 28 d	816:832	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	14	18	theme	milk	1966:1969	arg1	sdLys					1985:1989	milk N. Increasing sdLys	1966:1989	milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d	1966:2118	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	14	19	dep	sdLys	1985:1989	arg1	decreased					2005:2013	decreased	2005:2013	decreased N balance as sdLys increased from 0 to 8 g/d	2005:2058	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	14	19	dep	sdLys	1985:1989	arg1	increased					2064:2072	increased	2064:2072	increased N balance as sdLys increased from 8 to 18 g/d	2064:2118	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	16	20	used	used	2438:2441	arg2	Lys					2412:2414	some Lys	2407:2414	some Lys	2407:2414	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	6	21	theme	Response	1048:1055	arg1	models					1065:1070	Response surface models	1048:1070	Response surface models	1048:1070	Response surface models were used to evaluate treatment responses.					
34364648	4	22	from	d	716:716	arg1	milk					721:724	milk	721:724	milk	721:724	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	10	23	theme	neutral	1510:1516	arg1	digestibility					1534:1546	neutral detergent fiber digestibility	1510:1546	neutral detergent fiber digestibility	1510:1546	Although neutral detergent fiber digestibility decreased as dietary starch increased, energy digestibility increased.					
34364648	1	24	theme	content	184:190	arg1	effects					138:144	The effects	134:144	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization	134:290	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	2	25	theme	DM	443:444	arg1	DM					414:415	DM	414:415	DM	414:415	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	25	theme	DM	443:444	arg1	sdLys					479:483	sdLys	479:483	sdLys	479:483	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	25	theme	DM	443:444	arg1	%					438:438	20.2 to 31.3%	426:438	20.2 to 31.3% of DM for starch	426:455	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	25	theme	DM	443:444	arg1	%					397:397	3.0 to 6.2%	387:397	3.0 to 6.2% of dry matter (DM) for FA	387:423	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	25	theme	DM	443:444	arg1	DM					443:444	DM	443:444	DM	443:444	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	25	theme	DM	443:444	arg1	matter					406:411	dry matter	402:411	dry matter (DM)	402:416	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	25	theme	DM	443:444	arg1	g/d					472:474	0 to 17.8 g/d	462:474	0 to 17.8 g/d of sdLys	462:483	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	8	26	theme	dietary	1186:1192	arg1	starch					1194:1199	dietary starch	1186:1199	dietary starch	1186:1199	When dietary starch was less than 24%, milk protein concentration increased with increasing sdLys, but when dietary starch was greater than 26% milk protein concentration decreased with increasing sdLys.					
34364648	16	27	theme	protein	2495:2501	arg1	expense					2479:2485	the expense	2475:2485	the expense of milk protein	2475:2501	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	4	28	theme	±	711:711	arg1	d					716:716	80 ± 14 d	708:716	mean ± SD; 80 ± 14 d in milk	697:724	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	2	29	theme	matter	406:411	arg1	DM					414:415	DM	414:415	DM	414:415	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	29	theme	matter	406:411	arg1	sdLys					479:483	sdLys	479:483	sdLys	479:483	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	29	theme	matter	406:411	arg1	%					438:438	20.2 to 31.3%	426:438	20.2 to 31.3% of DM for starch	426:455	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	29	theme	matter	406:411	arg1	%					397:397	3.0 to 6.2%	387:397	3.0 to 6.2% of dry matter (DM) for FA	387:423	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	29	theme	matter	406:411	arg1	DM					443:444	DM	443:444	DM	443:444	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	29	theme	matter	406:411	arg1	matter					406:411	dry matter	402:411	dry matter (DM)	402:416	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	29	theme	matter	406:411	arg1	g/d					472:474	0 to 17.8 g/d	462:474	0 to 17.8 g/d of sdLys	462:483	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	11	30	theme	energy	1658:1663	arg1	content					1670:1676	metabolizable energy (ME) content	1644:1676	metabolizable energy (ME) content	1644:1676	As dietary FA increased, metabolizable energy (ME) content quadratically increased.					
34364648	0	31	theme	lysine	55:60	arg1	supply					62:67	supplemental lysine supply	42:67	supplemental lysine supply	42:67	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	1	32	theme	N	278:278	arg1	utilization					280:290	N utilization	278:290	N utilization	278:290	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	10	33	theme	fiber	1528:1532	arg1	digestibility					1534:1546	neutral detergent fiber digestibility	1510:1546	neutral detergent fiber digestibility	1510:1546	Although neutral detergent fiber digestibility decreased as dietary starch increased, energy digestibility increased.					
34364648	15	34	theme	Increasing	2121:2130	arg1	FA					2140:2141	Increasing dietary FA	2121:2141	Increasing dietary FA	2121:2141	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	4	35	theme	balanced	758:765	arg1	block					778:782	a partially balanced incomplete block	746:782	a partially balanced incomplete block design	746:789	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	4	36	dep	SD	704:705	arg1	d					716:716	80 ± 14 d	708:716	mean ± SD; 80 ± 14 d in milk	697:724	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	1	37	theme	dietary	149:155	arg1	FA					169:170	FA	169:170	FA	169:170	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	1	37	theme	dietary	149:155	arg1	acid					163:166	dietary fatty acid	149:166	dietary fatty acid (FA)	149:171	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	8	38	theme	milk	1220:1223	arg1	concentration					1233:1245	milk protein concentration	1220:1245	milk protein concentration	1220:1245	When dietary starch was less than 24%, milk protein concentration increased with increasing sdLys, but when dietary starch was greater than 26% milk protein concentration decreased with increasing sdLys.					
34364648	5	39	theme	final	845:849	arg1	d					853:853	the final 4 d	841:853	the final 4 d	841:853	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	15	40	theme	dietary	2185:2191	arg1	intake					2210:2215	high dietary FA, decreased DM intake	2180:2215	intake	2210:2215	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	2	41	theme	different	333:341	arg1	amounts					343:349	5 different amounts	331:349	5 different amounts	331:349	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	14	42	theme	N.	1971:1972	arg1	sdLys					1985:1989	milk N. Increasing sdLys	1966:1989	milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d	1966:2118	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	0	43	theme	Dietary	0:6	arg1	acid					14:17	Dietary fatty acid	0:17	Dietary fatty acid	0:17	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	5	44	theme	feed	911:914	arg1	intake					916:921	feed intake	911:921	feed intake	911:921	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	15	45	from	plateau	2252:2258	arg1	content					2266:2272	ME content	2263:2272	ME content	2263:2272	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	15	45	from	plateau	2252:2258	arg1	supply					2295:2300	ME supply	2292:2300	ME supply	2292:2300	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	2	46	theme	factor	356:361	arg1	limits					363:368	factor limits	356:368	factor limits	356:368	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	16	47	theme	milk	2362:2365	arg1	protein					2367:2373	milk protein	2362:2373	milk protein	2362:2373	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	4	48	theme	block	778:782	arg1	design					784:789	a partially balanced incomplete block design	746:789	a partially balanced incomplete block design	746:789	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	3	49	theme	Dietary	486:492	arg1	FA					494:495	Dietary FA and starch	486:506	FA	494:495	Dietary FA and starch were increased by replacing soyhulls with supplemental fat and corn grain, respectively, and sdLys increased with rumen-protected Lys.					
34364648	4	50	theme	unique	651:656	arg1	treatments					658:667	Fifteen unique treatments	643:667	Fifteen unique treatments	643:667	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	14	51	theme	dietary	1952:1958	arg1	N					1960:1960	dietary N	1952:1960	dietary N	1952:1960	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	0	52	theme	starch	23:28	arg1	content					30:36	starch content	23:36	starch content	23:36	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	1	53	theme	energy	254:259	arg1	utilization					261:271	energy utilization	254:271	energy utilization	254:271	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	8	54	theme	dietary	1289:1295	arg1	starch					1297:1302	dietary starch	1289:1302	dietary starch	1289:1302	When dietary starch was less than 24%, milk protein concentration increased with increasing sdLys, but when dietary starch was greater than 26% milk protein concentration decreased with increasing sdLys.					
34364648	10	55	theme	dietary	1561:1567	arg1	starch					1569:1574	dietary starch	1561:1574	dietary starch	1561:1574	Although neutral detergent fiber digestibility decreased as dietary starch increased, energy digestibility increased.					
34364648	4	56	dep	cows	691:694	arg1	SD					704:705	mean ± SD	697:705	mean ± SD; 80 ± 14 d in milk	697:724	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	10	57	theme	energy	1587:1592	arg1	digestibility					1594:1606	energy digestibility	1587:1606	energy digestibility	1587:1606	Although neutral detergent fiber digestibility decreased as dietary starch increased, energy digestibility increased.					
34364648	15	58	from	decrease	2280:2287	arg1	content					2266:2272	ME content	2263:2272	ME content	2263:2272	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	15	58	from	decrease	2280:2287	arg1	supply					2295:2300	ME supply	2292:2300	ME supply	2292:2300	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	5	59	theme	headbox-style	969:981	arg1	calorimetry					992:1002	headbox-style indirect calorimetry	969:1002	headbox-style indirect calorimetry	969:1002	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	2	60	dep	31.3	434:437	arg1	to					431:432	to	431:432	to	431:432	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	6	61	used	used	1077:1080	arg2	models					1065:1070	Response surface models	1048:1070	Response surface models	1048:1070	Response surface models were used to evaluate treatment responses.					
34364648	15	62	theme	FA	2221:2222	arg1	digestibility					2224:2236	FA digestibility	2221:2236	FA digestibility	2221:2236	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	1	63	theme	digestible	216:225	arg1	Lys					227:229	supplemental digestible Lys	203:229	supplemental digestible Lys (sdLys)	203:237	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	1	63	theme	digestible	216:225	arg1	sdLys					232:236	sdLys	232:236	sdLys	232:236	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	8	64	theme	protein	1330:1336	arg1	concentration					1338:1350	milk protein concentration	1325:1350	milk protein concentration	1325:1350	When dietary starch was less than 24%, milk protein concentration increased with increasing sdLys, but when dietary starch was greater than 26% milk protein concentration decreased with increasing sdLys.					
34364648	13	65	theme	CH4	1853:1855	arg1	production					1857:1866	CH4 production	1853:1866	CH4 production	1853:1866	Increasing dietary FA and starch decreased CH4 production and urinary energy.					
34364648	12	66	theme	ME	1713:1714	arg1	Supply					1703:1708	Supply	1703:1708	Supply of ME	1703:1714	Supply of ME increased as dietary FA increased from 3.0 to 4.2% and decreased as FA increased beyond 4.2%.					
34364648	4	67	theme	mean	697:700	arg1	SD					704:705	mean ± SD	697:705	mean ± SD; 80 ± 14 d in milk	697:724	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	14	68	theme	dietary	1899:1905	arg1	starch					1907:1912	dietary starch	1899:1912	dietary starch	1899:1912	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	1	69	theme	fatty	157:161	arg1	FA					169:170	FA	169:170	FA	169:170	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	1	69	theme	fatty	157:161	arg1	acid					163:166	dietary fatty acid	149:166	dietary fatty acid (FA)	149:171	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	9	70	theme	FA	1402:1403	arg1	Digestibility					1385:1397	Digestibility	1385:1397	Digestibility of FA	1385:1403	Digestibility of FA increased when dietary FA increased from 3.0 to 4.2% and decreased as FA increased beyond 4.2%.					
34364648	7	71	theme	milk	1161:1164	arg1	yield					1174:1178	milk protein yield	1161:1178	milk protein yield	1161:1178	Increasing dietary FA decreased DM intake and milk protein yield.					
34364648	3	72	theme	supplemental	550:561	arg1	fat					563:565	supplemental fat	550:565	supplemental fat	550:565	Dietary FA and starch were increased by replacing soyhulls with supplemental fat and corn grain, respectively, and sdLys increased with rumen-protected Lys.					
34364648	16	73	theme	muscle	2447:2452	arg1	synthesis					2462:2470	muscle protein synthesis	2447:2470	muscle protein synthesis at the expense of milk protein	2447:2501	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	0	74	theme	Jersey	121:126	arg1	cows					128:131	lactating Jersey cows	111:131	lactating Jersey cows	111:131	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	2	75	dep	17.8	467:470	arg1	to					464:465	to	464:465	to	464:465	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	0	76	from	energy	76:81	arg1	cows					128:131	lactating Jersey cows	111:131	lactating Jersey cows	111:131	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	7	77	theme	decreased	1137:1145	arg1	intake					1150:1155	dietary FA decreased DM intake	1126:1155	dietary FA decreased DM intake	1126:1155	Increasing dietary FA decreased DM intake and milk protein yield.					
34364648	6	78	theme	surface	1057:1063	arg1	models					1065:1070	Response surface models	1048:1070	Response surface models	1048:1070	Response surface models were used to evaluate treatment responses.					
34364648	13	79	theme	dietary	1821:1827	arg1	FA					1829:1830	Increasing dietary FA	1810:1830	Increasing dietary FA	1810:1830	Increasing dietary FA and starch decreased CH4 production and urinary energy.					
34364648	10	80	theme	detergent	1518:1526	arg1	digestibility					1534:1546	neutral detergent fiber digestibility	1510:1546	neutral detergent fiber digestibility	1510:1546	Although neutral detergent fiber digestibility decreased as dietary starch increased, energy digestibility increased.					
34364648	1	81	theme	starch	177:182	arg1	content					184:190	starch content	177:190	starch content	177:190	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	7	82	theme	dietary	1126:1132	arg1	intake					1150:1155	dietary FA decreased DM intake	1126:1155	dietary FA decreased DM intake	1126:1155	Increasing dietary FA decreased DM intake and milk protein yield.					
34364648	9	83	dep	4.2	1453:1455	arg1	to					1450:1451	to	1450:1451	to	1450:1451	Digestibility of FA increased when dietary FA increased from 3.0 to 4.2% and decreased as FA increased beyond 4.2%.					
34364648	11	84	theme	ME	1666:1667	arg1	content					1670:1676	metabolizable energy (ME) content	1644:1676	metabolizable energy (ME) content	1644:1676	As dietary FA increased, metabolizable energy (ME) content quadratically increased.					
34364648	5	85	theme	N	1010:1010	arg1	utilization					1012:1022	N utilization	1010:1022	N utilization (via total collection)	1010:1045	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	15	86	theme	ME	2156:2157	arg1	content					2159:2165	ME content	2156:2165	ME content	2156:2165	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	0	87	from	nitrogen	87:94	arg1	cows					128:131	lactating Jersey cows	111:131	lactating Jersey cows	111:131	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	14	88	dep	18	2113:2114	arg1	to					2110:2111	to	2110:2111	to	2110:2111	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	16	89	from	expense	2479:2485	arg1	synthesis					2462:2470	muscle protein synthesis	2447:2470	muscle protein synthesis at the expense of milk protein	2447:2501	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	2	90	theme	dry	402:404	arg1	DM					414:415	DM	414:415	DM	414:415	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	90	theme	dry	402:404	arg1	matter					406:411	dry matter	402:411	dry matter (DM)	402:416	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	15	91	theme	dietary	2132:2138	arg1	FA					2140:2141	Increasing dietary FA	2121:2141	Increasing dietary FA	2121:2141	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	8	92	theme	protein	1225:1231	arg1	concentration					1233:1245	milk protein concentration	1220:1245	milk protein concentration	1220:1245	When dietary starch was less than 24%, milk protein concentration increased with increasing sdLys, but when dietary starch was greater than 26% milk protein concentration decreased with increasing sdLys.					
34364648	14	93	dep	8	2054:2054	arg1	to					2051:2052	to	2051:2052	to	2051:2052	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	14	94	theme	N	2015:2015	arg1	balance					2017:2023	N balance	2015:2023	N balance	2015:2023	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	13	95	theme	Increasing	1810:1819	arg1	FA					1829:1830	Increasing dietary FA	1810:1830	Increasing dietary FA	1810:1830	Increasing dietary FA and starch decreased CH4 production and urinary energy.					
34364648	6	96	theme	treatment	1094:1102	arg1	responses					1104:1112	treatment responses	1094:1112	treatment responses	1094:1112	Response surface models were used to evaluate treatment responses.					
34364648	5	97	theme	milk	878:881	arg1	production					883:892	milk production	878:892	milk production	878:892	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	2	98	dep	6.2	394:396	arg1	to					391:392	to	391:392	to	391:392	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	15	99	theme	decreased	2197:2205	arg1	intake					2210:2215	high dietary FA, decreased DM intake	2180:2215	intake	2210:2215	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	2	100	theme	sdLys	479:483	arg1	DM					414:415	DM	414:415	DM	414:415	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	100	theme	sdLys	479:483	arg1	sdLys					479:483	sdLys	479:483	sdLys	479:483	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	100	theme	sdLys	479:483	arg1	%					438:438	20.2 to 31.3%	426:438	20.2 to 31.3% of DM for starch	426:455	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	100	theme	sdLys	479:483	arg1	%					397:397	3.0 to 6.2%	387:397	3.0 to 6.2% of dry matter (DM) for FA	387:423	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	100	theme	sdLys	479:483	arg1	DM					443:444	DM	443:444	DM	443:444	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	100	theme	sdLys	479:483	arg1	matter					406:411	dry matter	402:411	dry matter (DM)	402:416	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	2	100	theme	sdLys	479:483	arg1	g/d					472:474	0 to 17.8 g/d	462:474	0 to 17.8 g/d of sdLys	462:483	Each factor was fed at 5 different amounts, and factor limits were as follows: 3.0 to 6.2% of dry matter (DM) for FA; 20.2 to 31.3% of DM for starch, and 0 to 17.8 g/d of sdLys.					
34364648	5	101	used	used	860:863	arg2	d					853:853	the final 4 d	841:853	the final 4 d	841:853	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	15	102	theme	ME	2292:2293	arg1	supply					2295:2300	ME supply	2292:2300	ME supply	2292:2300	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	1	103	from	effects	138:144	arg1	production					242:251	production	242:251	production	242:251	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	1	103	from	effects	138:144	arg1	utilization					261:271	energy utilization	254:271	energy utilization	254:271	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	1	103	from	effects	138:144	arg1	utilization					280:290	N utilization	278:290	N utilization	278:290	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	12	104	dep	4.2	1762:1764	arg1	to					1759:1760	to	1759:1760	to	1759:1760	Supply of ME increased as dietary FA increased from 3.0 to 4.2% and decreased as FA increased beyond 4.2%.					
34364648	14	105	theme	Increasing	1974:1983	arg1	sdLys					1985:1989	milk N. Increasing sdLys	1966:1989	milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d	1966:2118	Increasing dietary starch increased the efficiency of utilizing dietary N for milk N. Increasing sdLys quadratically decreased N balance as sdLys increased from 0 to 8 g/d and increased N balance as sdLys increased from 8 to 18 g/d.					
34364648	0	106	theme	fatty	8:12	arg1	acid					14:17	Dietary fatty acid	0:17	Dietary fatty acid	0:17	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	15	107	theme	high	2180:2183	arg1	intake					2210:2215	high dietary FA, decreased DM intake	2180:2215	intake	2210:2215	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	0	108	dep	energy	76:81	arg1	utilization					96:106	utilization	96:106	utilization	96:106	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	16	109	theme	milk	2490:2493	arg1	protein					2495:2501	milk protein	2490:2501	milk protein	2490:2501	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	5	110	theme	total	1029:1033	arg1	collection					1035:1044	total collection	1029:1044	total collection	1029:1044	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	11	111	theme	dietary	1622:1628	arg1	FA					1630:1631	dietary FA	1622:1631	dietary FA	1622:1631	As dietary FA increased, metabolizable energy (ME) content quadratically increased.					
34364648	15	112	theme	FA	2193:2194	arg1	intake					2210:2215	high dietary FA, decreased DM intake	2180:2215	intake	2210:2215	Increasing dietary FA can increase ME content, however, at high dietary FA, decreased DM intake and FA digestibility resulted in a plateau in ME content and a decrease in ME supply.					
34364648	5	113	theme	energy	924:929	arg1	utilization					931:941	energy utilization	924:941	energy utilization (via total collection and headbox-style indirect calorimetry)	924:1003	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
34364648	16	114	theme	dietary	2380:2386	arg1	starch					2388:2393	dietary starch	2380:2393	dietary starch	2380:2393	Our results demonstrate that sdLys supply is important for milk protein when dietary starch is low, and some Lys may be preferentially used for muscle protein synthesis at the expense of milk protein when sdLys is high.					
34364648	4	115	theme	incomplete	767:776	arg1	block					778:782	a partially balanced incomplete block	746:782	a partially balanced incomplete block design	746:789	Fifteen unique treatments were fed to 25 Jersey cows (mean ± SD; 80 ± 14 d in milk) across 3 blocks in a partially balanced incomplete block design.					
34364648	11	116	theme	metabolizable	1644:1656	arg1	content					1670:1676	metabolizable energy (ME) content	1644:1676	metabolizable energy (ME) content	1644:1676	As dietary FA increased, metabolizable energy (ME) content quadratically increased.					
34364648	0	117	theme	supplemental	42:53	arg1	supply					62:67	supplemental lysine supply	42:67	supplemental lysine supply	42:67	Dietary fatty acid and starch content and supplemental lysine supply affect energy and nitrogen utilization in lactating Jersey cows.					
34364648	13	118	theme	urinary	1872:1878	arg1	energy					1880:1885	urinary energy	1872:1885	urinary energy	1872:1885	Increasing dietary FA and starch decreased CH4 production and urinary energy.					
34364648	8	119	theme	milk	1325:1328	arg1	concentration					1338:1350	milk protein concentration	1325:1350	milk protein concentration	1325:1350	When dietary starch was less than 24%, milk protein concentration increased with increasing sdLys, but when dietary starch was greater than 26% milk protein concentration decreased with increasing sdLys.					
34364648	1	120	theme	supplemental	203:214	arg1	Lys					227:229	supplemental digestible Lys	203:229	supplemental digestible Lys (sdLys)	203:237	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	1	120	theme	supplemental	203:214	arg1	sdLys					232:236	sdLys	232:236	sdLys	232:236	The effects of dietary fatty acid (FA) and starch content as well as supplemental digestible Lys (sdLys) on production, energy utilization, and N utilization were evaluated.					
34364648	5	121	theme	indirect	983:990	arg1	calorimetry					992:1002	headbox-style indirect calorimetry	969:1002	headbox-style indirect calorimetry	969:1002	Each block consisted of 4 periods of 28 d, where the final 4 d were used to determine milk production and composition, feed intake, energy utilization (via total collection and headbox-style indirect calorimetry), and N utilization (via total collection).					
32867196	5	0	theme	ionic	764:768	arg1	composition					770:780	the ionic composition	760:780	the ionic composition of the solution	760:796	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	10	1	theme	phase	1520:1524	arg1	behavior					1526:1533	the DPPC phase behavior	1511:1533	the DPPC phase behavior	1511:1533	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	4	2	theme	phospholipids	481:493	arg1	interaction					451:461	the interaction	447:461	the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication	447:547	Within this study we focus on the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication.					
32867196	6	3	theme	small	1015:1019	arg1	scattering					1027:1036	small angle scattering	1015:1036	small angle scattering	1015:1036	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	3	theme	small	1015:1019	arg1	scattering					1003:1012	dynamic light scattering	989:1012	dynamic light scattering	989:1012	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	0	4	theme	Ions	62:65	arg1	Influence					0:8	Influence	0:8	Influence of the Molecular Weight	0:32	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	0	4	theme	Ions	62:65	arg1	Presence					42:49	the Presence	38:49	the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC	38:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	6	5	theme	zeta	913:916	arg1	potential					918:926	zeta potential	913:926	zeta potential	913:926	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	5	theme	zeta	913:916	arg1	lipids					870:875	lipids	870:875	lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility)	870:956	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	1	6	theme	essential	137:145	arg1	macromolecule					165:177	an essential physiological bio macromolecule	134:177	an essential physiological bio macromolecule with different functions	134:202	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	1	6	theme	essential	137:145	arg1	Hyaluronan					120:129	Hyaluronan	120:129	Hyaluronan	120:129	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	0	7	theme	Molecular	74:82	arg1	Interaction					84:94	the Molecular Interaction	70:94	the Molecular Interaction of Hyaluronan and DPPC	70:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	0	8	from	Influence	0:8	arg1	Interaction					84:94	the Molecular Interaction	70:94	the Molecular Interaction of Hyaluronan and DPPC	70:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	7	9	theme	phospholipids	1123:1135	arg1	interaction					1093:1103	The interaction	1089:1103	The interaction of hyaluronan and phospholipids	1089:1135	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	5	10	theme	association	594:604	arg1	structures					606:615	the association structures	590:615	the association structures	590:615	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	7	11	theme	strongest	1223:1231	arg1	interaction					1233:1243	the strongest interaction	1219:1243	the strongest interaction	1219:1243	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	0	12	from	Presence	42:49	arg1	Interaction					84:94	the Molecular Interaction	70:94	the Molecular Interaction of Hyaluronan and DPPC	70:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	9	13	theme	hydrogen	1452:1459	arg1	bonds					1461:1465	formed hydrogen bonds	1445:1465	formed hydrogen bonds between HA and DPPC	1445:1485	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	6	14	theme	light	997:1001	arg1	scattering					1027:1036	small angle scattering	1015:1036	small angle scattering	1015:1036	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	14	theme	light	997:1001	arg1	scattering					1003:1012	dynamic light scattering	989:1012	dynamic light scattering	989:1012	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	5	15	theme	bio	744:746	arg1	polymer					748:754	the bio polymer	740:754	the bio polymer	740:754	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	6	16	theme	scanning	891:898	arg1	calorimetry					900:910	differential scanning calorimetry	878:910	differential scanning calorimetry	878:910	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	16	theme	scanning	891:898	arg1	lipids					870:875	lipids	870:875	lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility)	870:956	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	7	17	theme	hyaluronan	1108:1117	arg1	interaction					1093:1103	The interaction	1089:1103	The interaction of hyaluronan and phospholipids	1089:1135	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	2	18	theme	lubrication	272:282	arg1	properties					284:293	remarkable lubrication properties	261:293	remarkable lubrication properties	261:293	One prominent area is the synovial fluid which exhibits remarkable lubrication properties.					
32867196	10	19	theme	carboxylate	1612:1622	arg1	groups					1624:1629	the carboxylate groups	1608:1629	the carboxylate groups	1608:1629	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	1	20	theme	physiological	147:159	arg1	macromolecule					165:177	an essential physiological bio macromolecule	134:177	an essential physiological bio macromolecule with different functions	134:202	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	1	20	theme	physiological	147:159	arg1	Hyaluronan					120:129	Hyaluronan	120:129	Hyaluronan	120:129	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	5	21	theme	polymer	748:754	arg1	composition					770:780	the ionic composition	760:780	the ionic composition of the solution	760:796	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	5	21	theme	polymer	748:754	arg1	weight					730:735	the molecular weight	716:735	the molecular weight of the bio polymer	716:754	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	6	22	theme	differential	878:889	arg1	calorimetry					900:910	differential scanning calorimetry	878:910	differential scanning calorimetry	878:910	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	22	theme	differential	878:889	arg1	lipids					870:875	lipids	870:875	lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility)	870:956	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	10	23	from	change	1501:1506	arg1	behavior					1526:1533	the DPPC phase behavior	1511:1533	the DPPC phase behavior	1511:1533	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	2	24	theme	remarkable	261:270	arg1	properties					284:293	remarkable lubrication properties	261:293	remarkable lubrication properties	261:293	One prominent area is the synovial fluid which exhibits remarkable lubrication properties.					
32867196	1	25	theme	bio	161:163	arg1	macromolecule					165:177	an essential physiological bio macromolecule	134:177	an essential physiological bio macromolecule with different functions	134:202	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	1	25	theme	bio	161:163	arg1	Hyaluronan					120:129	Hyaluronan	120:129	Hyaluronan	120:129	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	7	26	theme	lower	1192:1196	arg1	weight					1208:1213	lower molecular weight	1192:1213	lower molecular weight	1192:1213	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	9	27	theme	calcium	1347:1353	arg1	ions					1355:1358	calcium ions	1347:1358	calcium ions	1347:1358	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	7	28	with	hyaluronan	1176:1185	arg1	weight					1208:1213	lower molecular weight	1192:1213	lower molecular weight	1192:1213	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	6	29	with	behavior	858:865	arg1	investigation					974:986	structural investigation	963:986	structural investigation (dynamic light scattering, small angle scattering)	963:1037	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	29	with	behavior	858:865	arg1	simulations					1055:1065	theoretical simulations	1043:1065	theoretical simulations (molecular dynamics)	1043:1086	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	29	with	behavior	858:865	arg1	dynamics					1078:1085	molecular dynamics	1068:1085	molecular dynamics	1068:1085	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	3	30	theme	synergetic	397:406	arg1	fashion					408:414	a synergetic fashion	395:414	a synergetic fashion	395:414	However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion.					
32867196	6	31	theme	dynamic	989:995	arg1	scattering					1027:1036	small angle scattering	1015:1036	small angle scattering	1015:1036	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	31	theme	dynamic	989:995	arg1	scattering					1003:1012	dynamic light scattering	989:1012	dynamic light scattering	989:1012	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	9	32	theme	carboxylate	1385:1395	arg1	HA					1407:1408	HA	1407:1408	HA	1407:1408	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	9	32	theme	carboxylate	1385:1395	arg1	groups					1397:1402	the carboxylate groups	1381:1402	the carboxylate groups of HA	1381:1408	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	9	33	theme	formed	1445:1450	arg1	bonds					1461:1465	formed hydrogen bonds	1445:1465	formed hydrogen bonds between HA and DPPC	1445:1485	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	0	34	theme	Molecular	17:25	arg1	Weight					27:32	the Molecular Weight	13:32	the Molecular Weight	13:32	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	10	35	theme	binding	1638:1644	arg1	distribution					1646:1657	the binding distribution	1634:1657	the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine	1634:1719	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	1	36	theme	different	184:192	arg1	functions					194:202	different functions	184:202	different functions	184:202	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	10	37	theme	local	1558:1562	arg1	inversion					1571:1579	a local charge inversion	1556:1579	a local charge inversion	1556:1579	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	0	38	theme	Hyaluronan	99:108	arg1	Interaction					84:94	the Molecular Interaction	70:94	the Molecular Interaction of Hyaluronan and DPPC	70:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	6	39	theme	lipids	870:875	arg1	behavior					858:865	the phase behavior	848:865	the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics)	848:1086	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	9	40	theme	bonds	1461:1465	arg1	number					1435:1440	the number	1431:1440	the number of formed hydrogen bonds between HA and DPPC	1431:1485	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	6	41	theme	structural	963:972	arg1	investigation					974:986	structural investigation	963:986	structural investigation (dynamic light scattering, small angle scattering)	963:1037	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	5	42	theme	molecular	720:728	arg1	weight					730:735	the molecular weight	716:735	the molecular weight of the bio polymer	716:754	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	7	43	theme	molecular	1198:1206	arg1	weight					1208:1213	lower molecular weight	1192:1213	lower molecular weight	1192:1213	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	7	44	theme	molecular	1152:1160	arg1	weight					1162:1167	the molecular weight	1148:1167	the molecular weight	1148:1167	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	0	45	theme	Weight	27:32	arg1	Influence					0:8	Influence	0:8	Influence of the Molecular Weight	0:32	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	0	45	theme	Weight	27:32	arg1	Presence					42:49	the Presence	38:49	the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC	38:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	4	46	theme	key	524:526	arg1	role					528:531	a key role	522:531	a key role	522:531	Within this study we focus on the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication.					
32867196	0	47	theme	DPPC	114:117	arg1	Interaction					84:94	the Molecular Interaction	70:94	the Molecular Interaction of Hyaluronan and DPPC	70:117	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	4	48	theme	hyaluronan	466:475	arg1	interaction					451:461	the interaction	447:461	the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication	447:547	Within this study we focus on the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication.					
32867196	10	49	theme	charge	1564:1569	arg1	inversion					1571:1579	a local charge inversion	1556:1579	a local charge inversion	1556:1579	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	7	50	contain	has	1215:1217	arg2	interaction					1233:1243	the strongest interaction	1219:1243	the strongest interaction	1219:1243	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	7	50	contain	has	1215:1217	arg1	hyaluronan					1176:1185	hyaluronan	1176:1185	hyaluronan with lower molecular weight	1176:1213	The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction.					
32867196	6	51	theme	theoretical	1043:1053	arg1	simulations					1055:1065	theoretical simulations	1043:1065	theoretical simulations (molecular dynamics)	1043:1086	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	51	theme	theoretical	1043:1053	arg1	dynamics					1078:1085	molecular dynamics	1068:1085	molecular dynamics	1068:1085	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	5	52	theme	solution	789:796	arg1	composition					770:780	the ionic composition	760:780	the ionic composition of the solution	760:796	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	5	52	theme	solution	789:796	arg1	weight					730:735	the molecular weight	716:735	the molecular weight of the bio polymer	716:754	We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution.					
32867196	3	53	theme	different	358:366	arg1	macromolecules					368:381	different macromolecules	358:381	different macromolecules	358:381	However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion.					
32867196	1	54	with	macromolecule	165:177	arg1	functions					194:202	different functions	184:202	different functions	184:202	Hyaluronan is an essential physiological bio macromolecule with different functions.					
32867196	6	55	theme	phase	852:856	arg1	behavior					858:865	the phase behavior	848:865	the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics)	848:1086	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	8	56	theme	ions	1315:1318	arg1	presence					1295:1302	the presence	1291:1302	the presence of calcium ions	1291:1318	Furthermore, the interaction is increased by the presence of calcium ions.					
32867196	10	57	theme	calcium	1584:1590	arg1	ions					1592:1595	calcium ions	1584:1595	calcium ions binding to the carboxylate groups	1584:1629	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	6	58	dep	lipids	870:875	arg1	calorimetry					900:910	differential scanning calorimetry	878:910	differential scanning calorimetry	878:910	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	58	dep	lipids	870:875	arg1	potential					918:926	zeta potential	913:926	zeta potential	913:926	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	58	dep	lipids	870:875	arg1	mobility					948:955	electrophoretic mobility	932:955	electrophoretic mobility	932:955	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	58	dep	lipids	870:875	arg1	lipids					870:875	lipids	870:875	lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility)	870:956	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	3	59	theme	synovial	309:316	arg1	fluid					318:322	the synovial fluid	305:322	the synovial fluid	305:322	However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion.					
32867196	3	59	theme	synovial	309:316	arg1	system					345:350	a multi-component system	327:350	a multi-component system where different macromolecules interact in a synergetic fashion	327:414	However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion.					
32867196	6	60	dep	investigation	974:986	arg1	scattering					1027:1036	small angle scattering	1015:1036	small angle scattering	1015:1036	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	60	dep	investigation	974:986	arg1	scattering					1003:1012	dynamic light scattering	989:1012	dynamic light scattering	989:1012	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	2	61	theme	prominent	209:217	arg1	fluid					240:244	the synovial fluid	227:244	the synovial fluid which exhibits remarkable lubrication properties	227:293	One prominent area is the synovial fluid which exhibits remarkable lubrication properties.					
32867196	2	61	theme	prominent	209:217	arg1	area					219:222	One prominent area	205:222	One prominent area	205:222	One prominent area is the synovial fluid which exhibits remarkable lubrication properties.					
32867196	8	62	theme	calcium	1307:1313	arg1	ions					1315:1318	calcium ions	1307:1318	calcium ions	1307:1318	Furthermore, the interaction is increased by the presence of calcium ions.					
32867196	6	63	theme	electrophoretic	932:946	arg1	mobility					948:955	electrophoretic mobility	932:955	electrophoretic mobility	932:955	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	63	theme	electrophoretic	932:946	arg1	lipids					870:875	lipids	870:875	lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility)	870:956	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	10	64	theme	observed	1492:1499	arg1	change					1501:1506	The observed change	1488:1506	The observed change in the DPPC phase behavior	1488:1533	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	10	65	theme	hyaluronan	1662:1671	arg1	distribution					1646:1657	the binding distribution	1634:1657	the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine	1634:1719	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	9	66	theme	HA	1407:1408	arg1	HA					1407:1408	HA	1407:1408	HA	1407:1408	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	9	66	theme	HA	1407:1408	arg1	groups					1397:1402	the carboxylate groups	1381:1402	the carboxylate groups of HA	1381:1408	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	0	67	theme	Calcium	54:60	arg1	Ions					62:65	Calcium Ions	54:65	Calcium Ions	54:65	Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC.					
32867196	10	68	theme	1,2-dipalmitoyl-sn-glycero-3-phosphocholine	1677:1719	arg1	distribution					1646:1657	the binding distribution	1634:1657	the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine	1634:1719	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	6	69	theme	molecular	1068:1076	arg1	simulations					1055:1065	theoretical simulations	1043:1065	theoretical simulations (molecular dynamics)	1043:1086	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	69	theme	molecular	1068:1076	arg1	dynamics					1078:1085	molecular dynamics	1068:1085	molecular dynamics	1068:1085	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	10	70	theme	DPPC	1515:1518	arg1	behavior					1526:1533	the DPPC phase behavior	1511:1533	the DPPC phase behavior	1511:1533	The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.					
32867196	3	71	theme	multi-component	329:343	arg1	fluid					318:322	the synovial fluid	305:322	the synovial fluid	305:322	However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion.					
32867196	3	71	theme	multi-component	329:343	arg1	system					345:350	a multi-component system	327:350	a multi-component system where different macromolecules interact in a synergetic fashion	327:414	However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion.					
32867196	9	72	dep	located	1364:1370	arg1	reduce					1424:1429	reduce	1424:1429	reduce the number of formed hydrogen bonds between HA and DPPC	1424:1485	Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC.					
32867196	2	73	theme	synovial	231:238	arg1	fluid					240:244	the synovial fluid	227:244	the synovial fluid which exhibits remarkable lubrication properties	227:293	One prominent area is the synovial fluid which exhibits remarkable lubrication properties.					
32867196	2	73	theme	synovial	231:238	arg1	area					219:222	One prominent area	205:222	One prominent area	205:222	One prominent area is the synovial fluid which exhibits remarkable lubrication properties.					
32867196	6	74	theme	angle	1021:1025	arg1	scattering					1027:1036	small angle scattering	1015:1036	small angle scattering	1015:1036	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32867196	6	74	theme	angle	1021:1025	arg1	scattering					1003:1012	dynamic light scattering	989:1012	dynamic light scattering	989:1012	We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics).					
32736272	7	0	theme	highest	1345:1351	arg1	concentrations					1353:1366	the highest concentrations	1341:1366	the highest concentrations tested	1341:1373	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	3	1	theme	design	544:549	arg1	parameters					551:560	the design parameters	540:560	the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria	540:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	8	2	with	oligomers	1398:1406	arg1	conformations					1422:1434	extended conformations	1413:1434	extended conformations	1413:1434	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	3	theme	extended	1413:1420	arg1	conformations					1422:1434	extended conformations	1413:1434	extended conformations	1413:1434	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	9	4	theme	materials	1845:1853	arg1	design					1824:1829	the design	1820:1829	the design of functional materials	1820:1853	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	4	5	theme	favorable	708:716	arg1	characteristics					733:747	favorable conformational characteristics	708:747	favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	708:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	2	6	theme	peptidoglycan	302:314	arg1	layer					316:320	the peptidoglycan layer	298:320	the peptidoglycan layer of S. aureus	298:333	Therapies that specifically target the peptidoglycan layer of S. aureus have emerged as new treatment avenues, towards which bacteria are less likely to develop resistance.					
32736272	7	7	theme	average	1218:1224	arg1	activity					1240:1247	average antibacterial activity	1218:1247	average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively	1218:1335	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	6	8	theme	photo-induced	1087:1099	arg1	transfer					1106:1113	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	3	9	theme	outer	607:611	arg1	layer					613:617	peptidoglycan outer layer	593:617	peptidoglycan outer layer of Gram-positive bacteria	593:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	9	10	theme	drug-resistant	1881:1894	arg1	bacteria					1896:1903	drug-resistant bacteria	1881:1903	drug-resistant bacteria	1881:1903	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	3	11	contain	have	491:494	arg1	majority					446:453	the majority	442:453	the majority of antibacterial polymers/oligomers	442:489	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	3	11	contain	have	491:494	arg2	ability					500:506	the ability to disrupt bacterial membranes	496:537	the ability to disrupt bacterial membranes	496:537	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	4	12	theme	structures	692:701	arg1	design					671:676	the design	667:676	the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	667:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	5	13	theme	cationic	940:947	arg1	oligomers					949:957	cationic oligomers	940:957	cationic oligomers displaying collapsed and expanded conformations	940:1005	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	4	14	theme	peptidoglycan	780:792	arg1	layer					800:804	the peptidoglycan outer layer	776:804	the peptidoglycan outer layer of Gram-positive bacteria	776:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	3	15	theme	bacteria	636:643	arg1	layer					613:617	peptidoglycan outer layer	593:617	peptidoglycan outer layer of Gram-positive bacteria	593:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	6	16	theme	triblock	1039:1046	arg1	oligomers					1057:1065	The most promising diblock and triblock cationic oligomers	1008:1065	oligomers	1057:1065	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	4	17	theme	layer	800:804	arg1	disruption					762:771	improved disruption	753:771	improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	753:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	1	18	theme	aureus	163:168	arg1	populations					182:192	Staphylococcus aureus (S. aureus) populations	148:192	Staphylococcus aureus (S. aureus) populations	148:192	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	8	19	theme	characterization	1613:1628	arg1	electron					1660:1667	scanning electron	1651:1667	scanning electron	1651:1667	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	19	theme	characterization	1613:1628	arg1	microscopy					1696:1705	atomic force microscopy	1683:1705	atomic force microscopy	1683:1705	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	19	theme	characterization	1613:1628	arg1	confocal					1670:1677	confocal	1670:1677	confocal	1670:1677	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	19	theme	characterization	1613:1628	arg1	techniques					1630:1639	characterization techniques	1613:1639	characterization techniques including scanning electron, confocal and atomic force microscopy	1613:1705	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	20	theme	higher	1457:1462	arg1	disruption					1464:1473	significantly higher disruption	1443:1473	significantly higher disruption of the peptidoglycan outer layer of S. aureus	1443:1519	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	0	21	theme	Gram-positive	75:87	arg1	bacteria					89:96	Gram-positive bacteria	75:96	Gram-positive bacteria	75:96	Conformationally tuned antibacterial oligomers target the peptidoglycan of Gram-positive bacteria.					
32736272	6	22	theme	photo	1139:1143	arg1	polymerization					1123:1136	radical polymerization	1115:1136	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	6	22	theme	photo	1139:1143	arg1	ATRP					1145:1148	photo ATRP	1139:1148	photo ATRP	1139:1148	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	6	23	dep	transfer	1106:1113	arg1	polymerization					1123:1136	radical polymerization	1115:1136	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	6	23	dep	transfer	1106:1113	arg1	ATRP					1145:1148	photo ATRP	1139:1148	photo ATRP	1139:1148	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	5	24	theme	dynamics	855:862	arg1	simulations					864:874	Molecular dynamics simulations	845:874	Molecular dynamics simulations	845:874	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	8	25	theme	peptidoglycan	1482:1494	arg1	layer					1502:1506	the peptidoglycan outer layer	1478:1506	the peptidoglycan outer layer of S. aureus	1478:1519	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	4	26	theme	bacteria	823:830	arg1	layer					800:804	the peptidoglycan outer layer	776:804	the peptidoglycan outer layer of Gram-positive bacteria	776:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	8	27	theme	layer	1502:1506	arg1	disruption					1464:1473	significantly higher disruption	1443:1473	significantly higher disruption of the peptidoglycan outer layer of S. aureus	1443:1519	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	3	28	theme	antibacterial	458:470	arg1	polymers/oligomers					472:489	antibacterial polymers/oligomers	458:489	antibacterial polymers/oligomers	458:489	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	8	29	theme	atomic	1683:1688	arg1	microscopy					1696:1705	atomic force microscopy	1683:1705	atomic force microscopy	1683:1705	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	4	30	theme	Gram-positive	809:821	arg1	bacteria					823:830	Gram-positive bacteria	809:830	Gram-positive bacteria	809:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	9	31	theme	new	1727:1729	arg1	insight					1731:1737	new insight	1727:1737	new insight into the structure/property relationship of antibacterial materials	1727:1805	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	0	32	theme	antibacterial	23:35	arg1	oligomers					37:45	Conformationally tuned antibacterial oligomers	0:45	Conformationally tuned antibacterial oligomers	0:45	Conformationally tuned antibacterial oligomers target the peptidoglycan of Gram-positive bacteria.					
32736272	1	33	theme	antibiotic	118:127	arg1	resistance					129:138	antibiotic resistance	118:138	antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations	118:192	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	4	34	theme	improved	753:760	arg1	disruption					762:771	improved disruption	753:771	improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	753:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	8	35	theme	diblock	1533:1539	arg1	oligomers					1541:1549	diblock oligomers	1533:1549	diblock oligomers	1533:1549	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	9	36	theme	structure/property	1748:1765	arg1	relationship					1767:1778	the structure/property relationship	1744:1778	the structure/property relationship of antibacterial materials	1744:1805	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	7	37	theme	%	1264:1264	arg1	activity					1240:1247	average antibacterial activity	1218:1247	average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively	1218:1335	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	8	38	theme	collapsed	1561:1569	arg1	conformation					1571:1582	more collapsed conformation	1556:1582	more collapsed conformation	1556:1582	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	2	39	theme	treatment	355:363	arg1	avenues					365:371	new treatment avenues	351:371	new treatment avenues	351:371	Therapies that specifically target the peptidoglycan layer of S. aureus have emerged as new treatment avenues, towards which bacteria are less likely to develop resistance.					
32736272	1	40	theme	medical	244:250	arg1	challenge					252:260	a global medical challenge	235:260	Staph-based infections a global medical challenge	212:260	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	3	41	theme	enhanced	570:577	arg1	disruption					579:588	the enhanced disruption	566:588	the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria	566:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	9	42	theme	materials	1797:1805	arg1	relationship					1767:1778	the structure/property relationship	1744:1778	the structure/property relationship of antibacterial materials	1744:1805	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	7	43	theme	%	1255:1255	arg1	activity					1240:1247	average antibacterial activity	1218:1247	average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively	1218:1335	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	6	44	theme	promising	1017:1025	arg1	diblock					1027:1033	The most promising diblock and triblock cationic oligomers	1008:1065	diblock	1027:1033	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	9	45	theme	functional	1834:1843	arg1	materials					1845:1853	functional materials	1834:1853	functional materials	1834:1853	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	7	46	theme	antibacterial	1226:1238	arg1	activity					1240:1247	average antibacterial activity	1218:1247	average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively	1218:1335	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	5	47	theme	oligomers	949:957	arg1	composition					925:935	composition	925:935	composition	925:935	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	5	47	theme	oligomers	949:957	arg1	design					914:919	structure design	904:919	structure design	904:919	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	3	48	theme	layer	613:617	arg1	disruption					579:588	the enhanced disruption	566:588	the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria	566:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	5	49	theme	collapsed	970:978	arg1	conformations					993:1005	collapsed and expanded conformations	970:1005	collapsed and expanded conformations	970:1005	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	6	50	theme	atom	1101:1104	arg1	transfer					1106:1113	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	4	51	theme	conformational	718:731	arg1	characteristics					733:747	favorable conformational characteristics	708:747	favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	708:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	7	52	theme	triblock	1189:1196	arg1	oligomers					1198:1206	the diblock and triblock oligomers	1173:1206	the diblock and triblock oligomers	1173:1206	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	3	53	theme	peptidoglycan	593:605	arg1	layer					613:617	peptidoglycan outer layer	593:617	peptidoglycan outer layer of Gram-positive bacteria	593:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	1	54	theme	Staph-based	212:222	arg1	infections					224:233	Staph-based infections a global medical challenge	212:260	Staph-based infections a global medical challenge	212:260	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	7	55	theme	diblock	1177:1183	arg1	oligomers					1198:1206	the diblock and triblock oligomers	1173:1206	the diblock and triblock oligomers	1173:1206	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
32736272	9	56	theme	bacteria	1896:1903	arg1	rise					1873:1876	the rise	1869:1876	the rise of drug-resistant bacteria	1869:1903	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	4	57	theme	oligomeric	681:690	arg1	structures					692:701	oligomeric structures	681:701	oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	681:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	3	58	theme	Gram-positive	622:634	arg1	bacteria					636:643	Gram-positive bacteria	622:643	Gram-positive bacteria	622:643	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	1	59	theme	resistance	129:138	arg1	rise					110:113	The recent rise	99:113	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations	99:192	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	6	60	theme	cationic	1048:1055	arg1	oligomers					1057:1065	The most promising diblock and triblock cationic oligomers	1008:1065	oligomers	1057:1065	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	9	61	theme	antibacterial	1783:1795	arg1	materials					1797:1805	antibacterial materials	1783:1805	antibacterial materials	1783:1805	This work provides new insight into the structure/property relationship of antibacterial materials and advances the design of functional materials for combating the rise of drug-resistant bacteria.					
32736272	8	62	theme	techniques	1630:1639	arg1	electron					1660:1667	scanning electron	1651:1667	scanning electron	1651:1667	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	62	theme	techniques	1630:1639	arg1	microscopy					1696:1705	atomic force microscopy	1683:1705	atomic force microscopy	1683:1705	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	62	theme	techniques	1630:1639	arg1	confocal					1670:1677	confocal	1670:1677	confocal	1670:1677	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	62	theme	techniques	1630:1639	arg1	number					1603:1608	a number	1601:1608	a number of characterization techniques including scanning electron, confocal and atomic force microscopy	1601:1705	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	4	63	theme	outer	794:798	arg1	layer					800:804	the peptidoglycan outer layer	776:804	the peptidoglycan outer layer of Gram-positive bacteria	776:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	0	64	theme	bacteria	89:96	arg1	peptidoglycan					58:70	the peptidoglycan	54:70	the peptidoglycan of Gram-positive bacteria	54:96	Conformationally tuned antibacterial oligomers target the peptidoglycan of Gram-positive bacteria.					
32736272	5	65	theme	expanded	984:991	arg1	conformations					993:1005	collapsed and expanded conformations	970:1005	collapsed and expanded conformations	970:1005	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	8	66	theme	scanning	1651:1658	arg1	electron					1660:1667	scanning electron	1651:1667	scanning electron	1651:1667	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	5	67	theme	Molecular	845:853	arg1	simulations					864:874	Molecular dynamics simulations	845:874	Molecular dynamics simulations	845:874	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	2	68	theme	aureus	328:333	arg1	layer					316:320	the peptidoglycan layer	298:320	the peptidoglycan layer of S. aureus	298:333	Therapies that specifically target the peptidoglycan layer of S. aureus have emerged as new treatment avenues, towards which bacteria are less likely to develop resistance.					
32736272	6	69	theme	radical	1115:1121	arg1	polymerization					1123:1136	radical polymerization	1115:1136	photo-induced atom transfer radical polymerization (photo ATRP)	1087:1149	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	6	69	theme	radical	1115:1121	arg1	ATRP					1145:1148	photo ATRP	1139:1148	photo ATRP	1139:1148	The most promising diblock and triblock cationic oligomers were synthesized by photo-induced atom transfer radical polymerization (photo ATRP).					
32736272	1	70	dep	aureus	163:168	arg1	aureus					174:179	S. aureus	171:179	S. aureus	171:179	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	3	71	theme	bacterial	519:527	arg1	membranes					529:537	bacterial membranes	519:537	bacterial membranes	519:537	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	8	72	theme	force	1690:1694	arg1	microscopy					1696:1705	atomic force microscopy	1683:1705	atomic force microscopy	1683:1705	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	73	theme	outer	1496:1500	arg1	layer					1502:1506	the peptidoglycan outer layer	1478:1506	the peptidoglycan outer layer of S. aureus	1478:1519	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	3	74	theme	polymers/oligomers	472:489	arg1	majority					446:453	the majority	442:453	the majority of antibacterial polymers/oligomers	442:489	While the majority of antibacterial polymers/oligomers have the ability to disrupt bacterial membranes, the design parameters for the enhanced disruption of peptidoglycan outer layer of Gram-positive bacteria remain unclear.					
32736272	0	75	theme	tuned	17:21	arg1	oligomers					37:45	Conformationally tuned antibacterial oligomers	0:45	Conformationally tuned antibacterial oligomers	0:45	Conformationally tuned antibacterial oligomers target the peptidoglycan of Gram-positive bacteria.					
32736272	1	76	theme	recent	103:108	arg1	rise					110:113	The recent rise	99:113	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations	99:192	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	5	77	theme	structure	904:912	arg1	design					914:919	structure design	904:919	structure design	904:919	Molecular dynamics simulations were employed to inform the structure design and composition of cationic oligomers displaying collapsed and expanded conformations.					
32736272	8	78	theme	aureus	1514:1519	arg1	layer					1502:1506	the peptidoglycan outer layer	1478:1506	the peptidoglycan outer layer of S. aureus	1478:1519	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	4	79	with	structures	692:701	arg1	characteristics					733:747	favorable conformational characteristics	708:747	favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria	708:830	Here, the design of oligomeric structures with favorable conformational characteristics for improved disruption of the peptidoglycan outer layer of Gram-positive bacteria is reported.					
32736272	8	80	dep	showed	1436:1441	arg1	compared					1521:1528	compared	1521:1528	compared	1521:1528	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	8	81	theme	triblock	1389:1396	arg1	oligomers					1398:1406	triblock oligomers	1389:1406	triblock oligomers with extended conformations	1389:1434	Importantly, triblock oligomers with extended conformations showed significantly higher disruption of the peptidoglycan outer layer of S. aureus compared to diblock oligomers with more collapsed conformation, as evidenced by a number of characterization techniques including scanning electron, confocal and atomic force microscopy.					
32736272	2	82	theme	new	351:353	arg1	avenues					365:371	new treatment avenues	351:371	new treatment avenues	351:371	Therapies that specifically target the peptidoglycan layer of S. aureus have emerged as new treatment avenues, towards which bacteria are less likely to develop resistance.					
32736272	1	83	theme	global	237:242	arg1	challenge					252:260	a global medical challenge	235:260	Staph-based infections a global medical challenge	212:260	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	1	84	dep	infections	224:233	arg1	challenge					252:260	a global medical challenge	235:260	Staph-based infections a global medical challenge	212:260	The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge.					
32736272	7	85	theme	methicillin-resistant S.	1284:1307	arg1	aureus 					1309:1315	methicillin-resistant S. aureus 	1284:1315	methicillin-resistant S. aureus 	1284:1315	Following synthesis, the diblock and triblock oligomers displayed average antibacterial activity of ~99% and ~98% for S. aureus and methicillin-resistant S. aureus (MRSA), respectively, at the highest concentrations tested.					
35181066	3	0	theme	body	478:481	arg1	weight					483:488	body weight	478:488	body weight	478:488	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	4	1	theme	increased	629:637	arg1	length					645:650	increased colon length	629:650	increased colon length	629:650	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	8	2	from	regulation	1560:1569	arg1	apoptosis					1518:1526	cell apoptosis	1513:1526	cell apoptosis	1513:1526	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	6	3	from	increase	1001:1008	arg1	abundance					1022:1030	relative abundance	1013:1030	relative abundance of the family Lachnospiraceae	1013:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	2	4	theme	peptide	258:264	arg1	LPF					297:299	LPF	297:299	LPF	297:299	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	2	4	theme	peptide	258:264	arg1	leucine-proline-phenylalanine					266:294	the walnut derived peptide leucine-proline-phenylalanine	239:294	the walnut derived peptide leucine-proline-phenylalanine (LPF)	239:300	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	7	5	theme	DSS	1387:1389	arg1	group					1391:1395	the DSS group	1383:1395	the DSS group	1383:1395	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	2	6	dep	derived	250:256	arg1	walnut					243:248	walnut	243:248	walnut	243:248	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	3	7	theme	inflammatory	531:542	arg1	levels					553:558	serum inflammatory cytokine levels	525:558	serum inflammatory cytokine levels	525:558	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	4	8	theme	beneficial	599:608	arg1	effects					610:616	beneficial effects	599:616	beneficial effects	599:616	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	8	theme	beneficial	599:608	arg1	expansion					735:743	expansion	735:743	expansion of splenic regulatory T cells	735:773	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	8	theme	beneficial	599:608	arg1	length					645:650	increased colon length	629:650	increased colon length	629:650	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	8	theme	beneficial	599:608	arg1	apoptosis					674:682	reduced colonic cell apoptosis	653:682	reduced colonic cell apoptosis	653:682	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	8	theme	beneficial	599:608	arg1	production					695:704	decreased production	685:704	decreased production of inflammatory factors	685:728	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	2	9	theme	-induced	333:340	arg1	colitis					342:348	dextran sulfate sodium (DSS)-induced colitis	305:348	dextran sulfate sodium (DSS)-induced colitis in mice	305:356	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	2	10	theme	recovery	219:226	arg1	effects					228:234	the protective and recovery effects	200:234	effects	228:234	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	5	11	theme	rDNA	821:824	arg1	results					837:843	16S rDNA sequencing results	817:843	16S rDNA sequencing results	817:843	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	7	12	theme	genera	1354:1359	arg1	abundance					1276:1284	increased relative abundance	1257:1284	increased relative abundance of beneficial genera	1257:1305	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	7	12	theme	genera	1354:1359	arg1	abundance					1321:1329	decreased abundance	1311:1329	decreased abundance of potentially harmful genera	1311:1359	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	2	13	theme	protective	204:213	arg1	effects					228:234	the protective and recovery effects	200:234	effects	228:234	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	4	14	theme	reduced	653:659	arg1	apoptosis					674:682	reduced colonic cell apoptosis	653:682	reduced colonic cell apoptosis	653:682	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	0	15	theme	protein	66:72	arg1	effects					48:54	Anti-inflammatory and gut microbiota regulatory effects	0:54	Anti-inflammatory and gut microbiota regulatory effects of walnut protein	0:72	Anti-inflammatory and gut microbiota regulatory effects of walnut protein derived peptide LPF in vivo.					
35181066	8	16	theme	colitis	1459:1465	arg1	symptoms					1467:1474	colitis symptoms	1459:1474	colitis symptoms	1459:1474	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	4	17	theme	cell	669:672	arg1	apoptosis					674:682	reduced colonic cell apoptosis	653:682	reduced colonic cell apoptosis	653:682	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	0	18	theme	peptide	82:88	arg1	LPF					90:92	peptide LPF	82:92	peptide LPF	82:92	Anti-inflammatory and gut microbiota regulatory effects of walnut protein derived peptide LPF in vivo.					
35181066	5	19	theme	M	875:875	arg1	LPF					870:872	100 mg/kg LPF	860:872	100 mg/kg LPF (M group)	860:882	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	5	19	theme	M	875:875	arg1	group					877:881	M group	875:881	M group	875:881	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	6	20	theme	partial	956:962	arg1	recovery					964:971	partial recovery	956:971	partial recovery of diversity of microbiota	956:998	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	1	21	theme	Walnut	103:108	arg1	peptides					110:117	Walnut peptides	103:117	Walnut peptides	103:117	Walnut peptides have been reported to exhibit diverse activities.					
35181066	2	22	link	derived	250:256	arg1	LPF					297:299	LPF	297:299	LPF	297:299	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	2	22	link	derived	250:256	arg1	leucine-proline-phenylalanine					266:294	the walnut derived peptide leucine-proline-phenylalanine	239:294	the walnut derived peptide leucine-proline-phenylalanine (LPF)	239:300	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	7	23	theme	M	1178:1178	arg1	group					1180:1184	the M group	1174:1184	the M group	1174:1184	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	5	24	theme	colitis	928:934	arg1	mice					936:939	colitis mice	928:939	colitis mice	928:939	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	7	25	from	composition	1141:1151	arg1	group					1180:1184	the M group	1174:1184	the M group	1174:1184	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	4	26	theme	splenic	748:754	arg1	cells					769:773	splenic regulatory T cells	748:773	splenic regulatory T cells	748:773	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	2	27	from	effects	228:234	arg1	colitis					342:348	dextran sulfate sodium (DSS)-induced colitis	305:348	dextran sulfate sodium (DSS)-induced colitis in mice	305:356	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	7	28	theme	decreased	1311:1319	arg1	abundance					1321:1329	decreased abundance	1311:1329	decreased abundance of potentially harmful genera	1311:1359	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	3	29	theme	peptide	363:369	arg1	LPF					371:373	The peptide LPF	359:373	The peptide LPF	359:373	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	6	30	from	recovery	964:971	arg1	abundance					1022:1030	relative abundance	1013:1030	relative abundance of the family Lachnospiraceae	1013:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	4	31	theme	inflammatory	709:720	arg1	factors					722:728	inflammatory factors	709:728	inflammatory factors	709:728	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	3	32	theme	symptoms	401:408	arg1	severity					389:396	the severity	385:396	the severity of symptoms	385:408	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	0	33	theme	Anti-inflammatory	0:16	arg1	effects					48:54	Anti-inflammatory and gut microbiota regulatory effects	0:54	Anti-inflammatory and gut microbiota regulatory effects of walnut protein	0:72	Anti-inflammatory and gut microbiota regulatory effects of walnut protein derived peptide LPF in vivo.					
35181066	7	34	theme	genera	1300:1305	arg1	abundance					1276:1284	increased relative abundance	1257:1284	increased relative abundance of beneficial genera	1257:1305	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	7	34	theme	genera	1300:1305	arg1	abundance					1321:1329	decreased abundance	1311:1329	decreased abundance of potentially harmful genera	1311:1359	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	3	35	theme	colitis	442:448	arg1	phase					433:437	the development phase	417:437	the development phase of colitis	417:448	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	3	36	theme	disease	491:497	arg1	score					514:518	disease activity index score	491:518	disease activity index score	491:518	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	5	37	theme	microbiota	914:923	arg1	dysbiosis					897:905	the dysbiosis	893:905	the dysbiosis of gut microbiota in colitis mice	893:939	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	8	38	theme	anti-inflammatory	1529:1545	arg1	effects					1547:1553	anti-inflammatory effects	1529:1553	anti-inflammatory effects	1529:1553	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	7	39	theme	gut	1156:1158	arg1	microbiota					1160:1169	gut microbiota	1156:1169	gut microbiota	1156:1169	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	4	40	theme	recovery	786:793	arg1	period					795:800	the recovery period	782:800	the recovery period	782:800	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	0	41	theme	regulatory	37:46	arg1	effects					48:54	Anti-inflammatory and gut microbiota regulatory effects	0:54	Anti-inflammatory and gut microbiota regulatory effects of walnut protein	0:72	Anti-inflammatory and gut microbiota regulatory effects of walnut protein derived peptide LPF in vivo.					
35181066	7	42	theme	relative	1267:1274	arg1	abundance					1276:1284	increased relative abundance	1257:1284	increased relative abundance of beneficial genera	1257:1305	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	4	43	theme	T	767:767	arg1	cells					769:773	splenic regulatory T cells	748:773	splenic regulatory T cells	748:773	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	3	44	theme	activity	499:506	arg1	score					514:518	disease activity index score	491:518	disease activity index score	491:518	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	7	45	theme	colitis	1240:1246	arg1	period					1230:1235	the recovery period	1217:1235	the recovery period of colitis	1217:1246	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	8	46	theme	gut	1574:1576	arg1	microbiota					1578:1587	gut microbiota	1574:1587	gut microbiota	1574:1587	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	8	47	from	reduction	1500:1508	arg1	apoptosis					1518:1526	cell apoptosis	1513:1526	cell apoptosis	1513:1526	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	4	48	theme	treatment	575:583	arg1	groups					585:590	the treatment groups	571:590	the treatment groups	571:590	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	8	49	from	effects	1547:1553	arg1	apoptosis					1518:1526	cell apoptosis	1513:1526	cell apoptosis	1513:1526	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	6	50	from	Ruminococcaceae	1066:1080	arg1	abundance					1022:1030	relative abundance	1013:1030	relative abundance of the family Lachnospiraceae	1013:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	6	51	theme	Lachnospiraceae	1046:1060	arg1	abundance					1022:1030	relative abundance	1013:1030	relative abundance of the family Lachnospiraceae	1013:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	3	52	theme	index	508:512	arg1	score					514:518	disease activity index score	491:518	disease activity index score	491:518	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	2	53	theme	leucine-proline-phenylalanine	266:294	arg1	effects					228:234	the protective and recovery effects	200:234	effects	228:234	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	5	54	theme	sequencing	826:835	arg1	results					837:843	16S rDNA sequencing results	817:843	16S rDNA sequencing results	817:843	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	2	55	from	colitis	342:348	arg1	mice					353:356	mice	353:356	mice	353:356	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	3	56	theme	serum	525:529	arg1	levels					553:558	serum inflammatory cytokine levels	525:558	serum inflammatory cytokine levels	525:558	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	4	57	theme	colon	639:643	arg1	length					645:650	increased colon length	629:650	increased colon length	629:650	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	3	58	from	changes	467:473	arg1	score					514:518	disease activity index score	491:518	disease activity index score	491:518	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	3	58	from	changes	467:473	arg1	weight					483:488	body weight	478:488	body weight	478:488	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	3	58	from	changes	467:473	arg1	levels					553:558	serum inflammatory cytokine levels	525:558	serum inflammatory cytokine levels	525:558	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	8	59	theme	peptide	1414:1420	arg1	LPF					1422:1424	the peptide LPF	1410:1424	the peptide LPF	1410:1424	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	6	60	theme	colitis	1114:1120	arg1	phase					1105:1109	the development phase	1089:1109	the development phase of colitis	1089:1120	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	5	61	theme	16S	817:819	arg1	results					837:843	16S rDNA sequencing results	817:843	16S rDNA sequencing results	817:843	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	3	62	theme	cytokine	544:551	arg1	levels					553:558	serum inflammatory cytokine levels	525:558	serum inflammatory cytokine levels	525:558	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	2	63	theme	derived	250:256	arg1	LPF					297:299	LPF	297:299	LPF	297:299	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	2	63	theme	derived	250:256	arg1	leucine-proline-phenylalanine					266:294	the walnut derived peptide leucine-proline-phenylalanine	239:294	the walnut derived peptide leucine-proline-phenylalanine (LPF)	239:300	In this study, we investigated the protective and recovery effects of the walnut derived peptide leucine-proline-phenylalanine (LPF) on dextran sulfate sodium (DSS)-induced colitis in mice.					
35181066	6	64	theme	microbiota	989:998	arg1	diversity					976:984	diversity	976:984	diversity of microbiota	976:998	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	7	65	theme	harmful	1346:1352	arg1	genera					1354:1359	potentially harmful genera	1334:1359	potentially harmful genera	1334:1359	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	4	66	theme	decreased	685:693	arg1	production					695:704	decreased production	685:704	decreased production of inflammatory factors	685:728	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	5	67	theme	100 mg/kg	860:868	arg1	LPF					870:872	100 mg/kg LPF	860:872	100 mg/kg LPF (M group)	860:882	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	5	67	theme	100 mg/kg	860:868	arg1	group					877:881	M group	875:881	M group	875:881	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	6	68	theme	diversity	976:984	arg1	Ruminococcaceae					1066:1080	Ruminococcaceae	1066:1080	Ruminococcaceae during the development phase of colitis	1066:1120	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	6	68	theme	diversity	976:984	arg1	recovery					964:971	partial recovery	956:971	partial recovery of diversity of microbiota	956:998	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	6	68	theme	diversity	976:984	arg1	increase					1001:1008	increase	1001:1008	increase in relative abundance of the family Lachnospiraceae	1001:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	8	69	theme	symptoms	1467:1474	arg1	restoration					1480:1490	restoration	1480:1490	restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota	1480:1587	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	8	69	theme	symptoms	1467:1474	arg1	remission					1446:1454	remission	1446:1454	remission of colitis symptoms	1446:1474	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	1	70	theme	diverse	149:155	arg1	activities					157:166	diverse activities	149:166	diverse activities	149:166	Walnut peptides have been reported to exhibit diverse activities.					
35181066	4	71	theme	colonic	661:667	arg1	apoptosis					674:682	reduced colonic cell apoptosis	653:682	reduced colonic cell apoptosis	653:682	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	7	72	theme	microbiota	1160:1169	arg1	composition					1141:1151	the composition	1137:1151	the composition of gut microbiota in the M group	1137:1184	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	6	73	theme	family	1039:1044	arg1	Lachnospiraceae					1046:1060	the family Lachnospiraceae	1035:1060	the family Lachnospiraceae	1035:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	8	74	theme	cell	1513:1516	arg1	apoptosis					1518:1526	cell apoptosis	1513:1526	cell apoptosis	1513:1526	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	3	75	theme	development	421:431	arg1	phase					433:437	the development phase	417:437	the development phase of colitis	417:448	The peptide LPF mitigated the severity of symptoms during the development phase of colitis, as evidenced by changes in body weight, disease activity index score, and serum inflammatory cytokine levels.					
35181066	7	76	theme	beneficial	1289:1298	arg1	genera					1300:1305	beneficial genera	1289:1305	beneficial genera	1289:1305	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	5	77	theme	gut	910:912	arg1	microbiota					914:923	gut microbiota	910:923	gut microbiota	910:923	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	6	78	theme	relative	1013:1020	arg1	abundance					1022:1030	relative abundance	1013:1030	relative abundance of the family Lachnospiraceae	1013:1060	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	4	79	theme	factors	722:728	arg1	expansion					735:743	expansion	735:743	expansion of splenic regulatory T cells	735:773	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	79	theme	factors	722:728	arg1	production					695:704	decreased production	685:704	decreased production of inflammatory factors	685:728	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	79	theme	factors	722:728	arg1	apoptosis					674:682	reduced colonic cell apoptosis	653:682	reduced colonic cell apoptosis	653:682	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	79	theme	factors	722:728	arg1	length					645:650	increased colon length	629:650	increased colon length	629:650	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	7	80	theme	increased	1257:1265	arg1	abundance					1276:1284	increased relative abundance	1257:1284	increased relative abundance of beneficial genera	1257:1305	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	0	81	theme	walnut	59:64	arg1	protein					66:72	walnut protein	59:72	walnut protein	59:72	Anti-inflammatory and gut microbiota regulatory effects of walnut protein derived peptide LPF in vivo.					
35181066	6	82	theme	development	1093:1103	arg1	phase					1105:1109	the development phase	1089:1109	the development phase of colitis	1089:1120	This included partial recovery of diversity of microbiota, increase in relative abundance of the family Lachnospiraceae and Ruminococcaceae during the development phase of colitis.					
35181066	8	83	theme	microbiota	1578:1587	arg1	reduction					1500:1508	reduction	1500:1508	reduction in cell apoptosis	1500:1526	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	8	83	theme	microbiota	1578:1587	arg1	effects					1547:1553	anti-inflammatory effects	1529:1553	anti-inflammatory effects	1529:1553	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	8	83	theme	microbiota	1578:1587	arg1	regulation					1560:1569	regulation	1560:1569	regulation of gut microbiota	1560:1587	In summary, the peptide LPF could contribute to remission of colitis symptoms and restoration through reduction in cell apoptosis, anti-inflammatory effects, and regulation of gut microbiota.					
35181066	4	84	theme	regulatory	756:765	arg1	cells					769:773	splenic regulatory T cells	748:773	splenic regulatory T cells	748:773	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	5	85	from	dysbiosis	897:905	arg1	mice					936:939	colitis mice	928:939	colitis mice	928:939	Additionally, 16S rDNA sequencing results indicated that 100 mg/kg LPF (M group) reversed the dysbiosis of gut microbiota in colitis mice.					
35181066	7	86	theme	recovery	1221:1228	arg1	period					1230:1235	the recovery period	1217:1235	the recovery period of colitis	1217:1246	Additionally, the composition of gut microbiota in the M group was constantly improved during the recovery period of colitis, showing increased relative abundance of beneficial genera and decreased abundance of potentially harmful genera compared with that in the DSS group.					
35181066	4	87	theme	cells	769:773	arg1	expansion					735:743	expansion	735:743	expansion of splenic regulatory T cells	735:773	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	87	theme	cells	769:773	arg1	production					695:704	decreased production	685:704	decreased production of inflammatory factors	685:728	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	87	theme	cells	769:773	arg1	apoptosis					674:682	reduced colonic cell apoptosis	653:682	reduced colonic cell apoptosis	653:682	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
35181066	4	87	theme	cells	769:773	arg1	length					645:650	increased colon length	629:650	increased colon length	629:650	Moreover, the treatment groups showed beneficial effects, including increased colon length, reduced colonic cell apoptosis, decreased production of inflammatory factors, and expansion of splenic regulatory T cells during the recovery period.					
32490561	7	0	theme	potential	1164:1172	arg1	applications					1174:1185	potential applications	1164:1185	potential applications in bone regeneration	1164:1206	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	6	1	theme	alizarin	844:851	arg1	staining					859:866	alizarin red-D staining	844:866	alizarin red-D staining	844:866	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	2	theme	phosphatase	818:828	arg1	staining					830:837	alkaline phosphatase staining	809:837	alkaline phosphatase staining	809:837	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	3	theme	better	1047:1052	arg1	osteogenesis					1054:1065	better osteogenesis	1047:1065	better osteogenesis	1047:1065	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	5	4	theme	drug-loaded	676:686	arg1	scaffolds					688:696	drug-loaded scaffolds	676:696	drug-loaded scaffolds	676:696	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	2	5	theme	Osteoprogenitor	186:200	arg1	cells					202:206	Osteoprogenitor cells	186:206	Osteoprogenitor cells	186:206	Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering.					
32490561	5	6	theme	scaffolds	688:696	arg1	fabrication					661:671	the fabrication	657:671	the fabrication of drug-loaded scaffolds	657:696	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	4	7	theme	hydrogel	439:446	arg1	scaffold					448:455	a composite hydrogel scaffold	427:455	a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA)	427:512	In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing.					
32490561	4	8	theme	sodium	493:498	arg1	SA					510:511	SA	510:511	SA	510:511	In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing.					
32490561	4	8	theme	sodium	493:498	arg1	alginate					500:507	sodium alginate	493:507	sodium alginate (SA)	493:512	In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing.					
32490561	5	9	theme	osteogenic	635:644	arg1	Naringin					565:572	Naringin	565:572	Naringin (NG)	565:577	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	9	theme	osteogenic	635:644	arg1	factors					646:652	osteogenic factors	635:652	osteogenic factors in the fabrication of drug-loaded scaffolds	635:696	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	9	theme	osteogenic	635:644	arg1	peptide					607:613	calcitonin-gene-related peptide	583:613	calcitonin-gene-related peptide (CGRP)	583:620	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	4	10	theme	composite	429:437	arg1	scaffold					448:455	a composite hydrogel scaffold	427:455	a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA)	427:512	In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing.					
32490561	7	11	theme	biocompatible	1136:1148	arg1	material					1150:1157	a biocompatible material	1134:1157	a biocompatible material with potential applications in bone regeneration	1134:1206	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	7	11	theme	biocompatible	1136:1148	arg1	scaffold					1116:1123	the HA/SA scaffold	1106:1123	the HA/SA scaffold	1106:1123	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	5	12	theme	calcitonin-gene-related	583:605	arg1	Naringin					565:572	Naringin	565:572	Naringin (NG)	565:577	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	12	theme	calcitonin-gene-related	583:605	arg1	CGRP					616:619	CGRP	616:619	CGRP	616:619	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	12	theme	calcitonin-gene-related	583:605	arg1	peptide					607:613	calcitonin-gene-related peptide	583:613	calcitonin-gene-related peptide (CGRP)	583:620	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	12	theme	calcitonin-gene-related	583:605	arg1	factors					646:652	osteogenic factors	635:652	osteogenic factors in the fabrication of drug-loaded scaffolds	635:696	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	1	13	theme	Bone	104:107	arg1	engineering					116:126	Bone tissue engineering	104:126	Bone tissue engineering	104:126	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	1	13	theme	Bone	104:107	arg1	approach					143:150	a promising approach	131:150	a promising approach for tackling clinical challenges	131:183	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	8	14	theme	positive	1271:1278	arg1	proliferation					1280:1292	proliferation	1280:1292	proliferation	1280:1292	Meanwhile, NG and CGRP doping could result in better and more positive proliferation and differentiation.					
32490561	0	15	theme	three-dimensional	13:29	arg1	scaffolds					31:39	Drug-loading three-dimensional scaffolds	0:39	Drug-loading three-dimensional scaffolds	0:39	Drug-loading three-dimensional scaffolds based on hydroxyapatite-sodium alginate for bone regeneration.					
32490561	6	16	theme	culture	905:911	arg1	testing					800:806	cell counting kit-8 testing	780:806	cell counting kit-8 testing	780:806	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	16	theme	culture	905:911	arg1	staining					830:837	alkaline phosphatase staining	809:837	alkaline phosphatase staining	809:837	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	16	theme	culture	905:911	arg1	microscopy					768:777	scanning electron microscopy	750:777	scanning electron microscopy	750:777	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	16	theme	culture	905:911	arg1	staining					859:866	alizarin red-D staining	844:866	alizarin red-D staining	844:866	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	16	theme	culture	905:911	arg1	experiments					726:736	animal experiments	719:736	animal experiments	719:736	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	17	theme	alkaline	809:816	arg1	phosphatase					818:828	alkaline phosphatase	809:828	alkaline phosphatase staining	809:837	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	1	18	theme	tissue	109:114	arg1	engineering					116:126	Bone tissue engineering	104:126	Bone tissue engineering	104:126	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	1	18	theme	tissue	109:114	arg1	approach					143:150	a promising approach	131:150	a promising approach for tackling clinical challenges	131:183	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	0	19	theme	Drug-loading	0:11	arg1	scaffolds					31:39	Drug-loading three-dimensional scaffolds	0:39	Drug-loading three-dimensional scaffolds	0:39	Drug-loading three-dimensional scaffolds based on hydroxyapatite-sodium alginate for bone regeneration.					
32490561	6	20	theme	HA/SA/CGRP	1016:1025	arg1	scaffolds					1027:1035	the HA/SA/NG and HA/SA/CGRP scaffolds	999:1035	scaffolds	1027:1035	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	21	theme	animal	719:724	arg1	experiments					726:736	animal experiments	719:736	animal experiments	719:736	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	22	theme	stem	895:898	arg1	culture					905:911	bone marrow mesenchymal stem cell culture	871:911	bone marrow mesenchymal stem cell culture	871:911	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	7	23	theme	HA/SA	1110:1114	arg1	material					1150:1157	a biocompatible material	1134:1157	a biocompatible material with potential applications in bone regeneration	1134:1206	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	7	23	theme	HA/SA	1110:1114	arg1	scaffold					1116:1123	the HA/SA scaffold	1106:1123	the HA/SA scaffold	1106:1123	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	8	24	theme	better	1255:1260	arg1	proliferation					1280:1292	proliferation	1280:1292	proliferation	1280:1292	Meanwhile, NG and CGRP doping could result in better and more positive proliferation and differentiation.					
32490561	5	25	used	used	627:630	arg2	CGRP					616:619	CGRP	616:619	CGRP	616:619	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	25	used	used	627:630	arg2	NG					575:576	NG	575:576	NG	575:576	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	25	used	used	627:630	arg2	Naringin					565:572	Naringin	565:572	Naringin (NG)	565:577	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	25	used	used	627:630	arg2	factors					646:652	osteogenic factors	635:652	osteogenic factors in the fabrication of drug-loaded scaffolds	635:696	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	5	25	used	used	627:630	arg2	peptide					607:613	calcitonin-gene-related peptide	583:613	calcitonin-gene-related peptide (CGRP)	583:620	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	6	26	theme	HA/SA/NG	1003:1010	arg1	scaffolds					1027:1035	the HA/SA/NG and HA/SA/CGRP scaffolds	999:1035	scaffolds	1027:1035	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	27	theme	cell	900:903	arg1	culture					905:911	bone marrow mesenchymal stem cell culture	871:911	bone marrow mesenchymal stem cell culture	871:911	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	7	28	with	material	1150:1157	arg1	applications					1174:1185	potential applications	1164:1185	potential applications in bone regeneration	1164:1206	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	0	29	theme	hydroxyapatite-sodium	50:70	arg1	alginate					72:79	hydroxyapatite-sodium alginate	50:79	hydroxyapatite-sodium alginate for bone regeneration	50:101	Drug-loading three-dimensional scaffolds based on hydroxyapatite-sodium alginate for bone regeneration.					
32490561	5	30	from	factors	646:652	arg1	fabrication					661:671	the fabrication	657:671	the fabrication of drug-loaded scaffolds	657:696	Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds.					
32490561	2	31	theme	osteogenic	209:218	arg1	factors					220:226	osteogenic factors	209:226	osteogenic factors	209:226	Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering.					
32490561	2	32	theme	bone	290:293	arg1	engineering					302:312	bone tissue engineering	290:312	bone tissue engineering	290:312	Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering.					
32490561	3	33	theme	scaffold	324:331	arg1	materials					333:341	scaffold materials	324:341	scaffold materials	324:341	However, scaffold materials remain limited due to their source, low biocompatibility, and so on.					
32490561	6	34	theme	mesenchymal	883:893	arg1	culture					905:911	bone marrow mesenchymal stem cell culture	871:911	bone marrow mesenchymal stem cell culture	871:911	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	35	theme	kit-8	794:798	arg1	testing					800:806	cell counting kit-8 testing	780:806	cell counting kit-8 testing	780:806	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	36	theme	HA/SA	1084:1088	arg1	scaffold					1090:1097	the HA/SA scaffold	1080:1097	the HA/SA scaffold	1080:1097	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	4	37	theme	three-dimensional	537:553	arg1	printing					555:562	three-dimensional printing	537:562	three-dimensional printing	537:562	In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing.					
32490561	6	38	theme	marrow	876:881	arg1	culture					905:911	bone marrow mesenchymal stem cell culture	871:911	bone marrow mesenchymal stem cell culture	871:911	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	39	theme	counting	785:792	arg1	testing					800:806	cell counting kit-8 testing	780:806	cell counting kit-8 testing	780:806	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	1	40	theme	promising	133:141	arg1	engineering					116:126	Bone tissue engineering	104:126	Bone tissue engineering	104:126	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	1	40	theme	promising	133:141	arg1	approach					143:150	a promising approach	131:150	a promising approach for tackling clinical challenges	131:183	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	7	41	theme	bone	1190:1193	arg1	regeneration					1195:1206	bone regeneration	1190:1206	bone regeneration	1190:1206	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	6	42	theme	bone	871:874	arg1	marrow					876:881	bone marrow	871:881	bone marrow mesenchymal stem cell culture	871:911	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	43	theme	cell	780:783	arg1	testing					800:806	cell counting kit-8 testing	780:806	cell counting kit-8 testing	780:806	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	44	theme	physicochemical	963:977	arg1	properties					979:988	similar physicochemical properties	955:988	similar physicochemical properties	955:988	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	2	45	theme	osteoinductive/osteoconductive	233:262	arg1	scaffolds					264:272	osteoinductive/osteoconductive scaffolds	233:272	osteoinductive/osteoconductive scaffolds	233:272	Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering.					
32490561	8	46	theme	CGRP	1227:1230	arg1	doping					1232:1237	CGRP doping	1227:1237	CGRP doping	1227:1237	Meanwhile, NG and CGRP doping could result in better and more positive proliferation and differentiation.					
32490561	7	47	from	applications	1174:1185	arg1	regeneration					1195:1206	bone regeneration	1190:1206	bone regeneration	1190:1206	Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration.					
32490561	3	48	theme	low	379:381	arg1	source					371:376	their source	365:376	their source	365:376	However, scaffold materials remain limited due to their source, low biocompatibility, and so on.					
32490561	3	48	theme	low	379:381	arg1	biocompatibility					383:398	low biocompatibility	379:398	low biocompatibility	379:398	However, scaffold materials remain limited due to their source, low biocompatibility, and so on.					
32490561	6	49	theme	red-D	853:857	arg1	staining					859:866	alizarin red-D staining	844:866	alizarin red-D staining	844:866	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	6	50	theme	electron	759:766	arg1	microscopy					768:777	scanning electron microscopy	750:777	scanning electron microscopy	750:777	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	1	51	theme	clinical	165:172	arg1	challenges					174:183	clinical challenges	165:183	clinical challenges	165:183	Bone tissue engineering is a promising approach for tackling clinical challenges.					
32490561	0	52	theme	bone	85:88	arg1	regeneration					90:101	bone regeneration	85:101	bone regeneration	85:101	Drug-loading three-dimensional scaffolds based on hydroxyapatite-sodium alginate for bone regeneration.					
32490561	6	53	theme	similar	955:961	arg1	properties					979:988	similar physicochemical properties	955:988	similar physicochemical properties	955:988	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
32490561	2	54	theme	tissue	295:300	arg1	engineering					302:312	bone tissue engineering	290:312	bone tissue engineering	290:312	Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering.					
32490561	6	55	theme	scanning	750:757	arg1	microscopy					768:777	scanning electron microscopy	750:777	scanning electron microscopy	750:777	Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold.					
34502525	2	0	theme	AMPK-glycogen	330:342	arg1	interactions					344:355	AMPK-glycogen interactions	330:355	AMPK-glycogen interactions	330:355	However, the physiological effects of disrupting AMPK-glycogen interactions remain incompletely understood.					
34502525	9	1	theme	mutation	1479:1486	arg1	effects					1455:1461	phenotypic effects	1444:1461	phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics	1444:1557	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	4	2	theme	DKI	628:630	arg1	mutation					632:639	this DKI mutation	623:639	this DKI mutation	623:639	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	0	3	theme	Tissue	117:122	arg1	Glycogen					124:131	Altered Tissue Glycogen	109:131	Altered Tissue Glycogen	109:131	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	7	4	theme	skeletal	1189:1196	arg1	muscle					1198:1203	skeletal muscle glycogen	1189:1212	skeletal muscle glycogen	1189:1212	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	5	5	theme	serum	796:800	arg1	hormone					802:808	serum hormone and lipid profiles	796:827	hormone	802:808	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	9	6	theme	phenotypic	1444:1453	arg1	effects					1455:1461	phenotypic effects	1444:1461	phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics	1444:1557	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	7	7	theme	relative	1318:1325	arg1	content					1310:1316	similar glycogen-associated protein content	1274:1316	similar glycogen-associated protein content relative to WT	1274:1331	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	7	8	contain	had	1078:1080	arg2	repletion					1176:1184	no repletion	1173:1184	no repletion of skeletal muscle glycogen	1173:1212	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	7	8	contain	had	1078:1080	arg2	content					1105:1111	reduced liver glycogen content	1082:1111	reduced liver glycogen content in the fed state concomitant with increased utilization	1082:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	7	8	contain	had	1078:1080	arg1	mice					1073:1076	DKI mice	1069:1076	DKI mice	1069:1076	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	9	9	theme	AMPK	1470:1473	arg1	mutation					1479:1486	the AMPK DKI mutation	1466:1486	the AMPK DKI mutation	1466:1486	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	6	10	theme	increased	961:969	arg1	mass					986:989	increased whole-body fat mass	961:989	increased whole-body fat mass	961:989	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	7	11	from	repletion	1176:1184	arg1	state					1124:1128	the fed state	1116:1128	the fed state concomitant with increased utilization	1116:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	7	12	theme	protein	1302:1308	arg1	content					1310:1316	similar glycogen-associated protein content	1274:1316	similar glycogen-associated protein content relative to WT	1274:1331	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	0	13	theme	Increased	62:70	arg1	Adiposity					72:80	Increased Adiposity	62:80	Increased Adiposity	62:80	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	3	14	dep	β1	553:554	arg1	isoforms					586:593	subunit isoforms	578:593	subunit isoforms	578:593	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	4	15	theme	substrate	655:663	arg1	utilization					665:675	whole-body substrate utilization	644:675	whole-body substrate utilization	644:675	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	3	16	from	binding	533:539	arg1	β2					568:569	β2	568:569	β2 (W98A)	568:576	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	3	16	from	binding	533:539	arg1	β1					553:554	the β1	549:554	the β1 (W100A)	549:562	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	3	16	from	binding	533:539	arg1	W98A					572:575	W98A	572:575	W98A	572:575	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	3	16	from	binding	533:539	arg1	W100A					557:561	W100A	557:561	W100A	557:561	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	5	17	theme	protein	854:860	arg1	content					862:868	tissue glycogen and protein content	834:868	content	862:868	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	4	18	theme	tissue	703:708	arg1	dynamics					719:726	tissue glycogen dynamics	703:726	tissue glycogen dynamics	703:726	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	0	19	theme	Fat	91:93	arg1	Oxidation					95:103	Reduced Fat Oxidation	83:103	Reduced Fat Oxidation	83:103	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	5	20	theme	wild-type	921:929	arg1	mice					936:939	chow-fed male DKI and age-matched wild-type (WT) mice	887:939	mice	936:939	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	3	21	theme	double	442:447	arg1	mice					464:467	AMPK β double knock-in (DKI) mice	435:467	AMPK β double knock-in (DKI) mice	435:467	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	7	22	theme	concomitant	1130:1140	arg1	state					1124:1128	the fed state	1116:1128	the fed state concomitant with increased utilization	1116:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	0	23	theme	AMPK-Glycogen	35:47	arg1	Binding					49:55	AMPK-Glycogen Binding	35:55	AMPK-Glycogen Binding	35:55	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	5	24	theme	WT	932:933	arg1	mice					936:939	chow-fed male DKI and age-matched wild-type (WT) mice	887:939	mice	936:939	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	7	25	theme	glycogen-associated	1282:1300	arg1	content					1310:1316	similar glycogen-associated protein content	1274:1316	similar glycogen-associated protein content relative to WT	1274:1331	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	3	26	theme	AMPK	435:438	arg1	β					440:440	AMPK β	435:440	AMPK β double knock-in (DKI) mice	435:467	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	7	27	theme	fed	1120:1122	arg1	state					1124:1128	the fed state	1116:1128	the fed state concomitant with increased utilization	1116:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	5	28	theme	male	896:899	arg1	DKI					901:903	chow-fed male DKI and age-matched wild-type (WT) mice	887:939	DKI	901:903	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	4	29	theme	glycogen	710:717	arg1	dynamics					719:726	tissue glycogen dynamics	703:726	tissue glycogen dynamics	703:726	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	0	30	theme	Whole-Body	10:19	arg1	Disruption					21:30	Whole-Body Disruption	10:30	Whole-Body Disruption of AMPK-Glycogen Binding	10:55	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	1	31	theme	β	270:270	arg1	subunit					272:278	its β subunit	266:278	its β subunit	266:278	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	9	32	theme	DKI	1475:1477	arg1	mutation					1479:1486	the AMPK DKI mutation	1466:1486	the AMPK DKI mutation	1466:1486	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	8	33	theme	skeletal	1348:1355	arg1	muscle					1357:1362	skeletal muscle	1348:1362	skeletal muscle	1348:1362	DKI liver and skeletal muscle displayed reductions in AMPK protein content versus WT.					
34502525	7	34	theme	glycogen	1096:1103	arg1	content					1105:1111	reduced liver glycogen content	1082:1111	reduced liver glycogen content in the fed state concomitant with increased utilization	1082:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	5	35	theme	metabolic	747:755	arg1	caging					757:762	metabolic caging	747:762	metabolic caging	747:762	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	6	36	theme	fat	982:984	arg1	mass					986:989	increased whole-body fat mass	961:989	increased whole-body fat mass	961:989	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	0	37	theme	Binding	49:55	arg1	Disruption					21:30	Whole-Body Disruption	10:30	Whole-Body Disruption of AMPK-Glycogen Binding	10:55	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	0	38	with	Mice	0:3	arg1	Disruption					21:30	Whole-Body Disruption	10:30	Whole-Body Disruption of AMPK-Glycogen Binding	10:55	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	1	39	theme	cellular	207:214	arg1	balance					223:229	cellular energy balance	207:229	cellular energy balance	207:229	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	40	theme	AMP-activated	147:159	arg1	regulator					194:202	a central regulator	184:202	a central regulator of cellular energy balance and metabolism	184:244	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	40	theme	AMP-activated	147:159	arg1	AMPK					177:180	AMPK	177:180	AMPK	177:180	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	40	theme	AMP-activated	147:159	arg1	kinase					169:174	AMP-activated protein kinase	147:174	The AMP-activated protein kinase (AMPK)	143:181	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	8	41	theme	protein	1393:1399	arg1	content					1401:1407	AMPK protein content	1388:1407	AMPK protein content	1388:1407	DKI liver and skeletal muscle displayed reductions in AMPK protein content versus WT.					
34502525	3	42	theme	critical	511:518	arg1	residues					502:509	residues	502:509	residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms	502:593	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	1	43	theme	balance	223:229	arg1	regulator					194:202	a central regulator	184:202	a central regulator of cellular energy balance and metabolism	184:244	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	43	theme	balance	223:229	arg1	kinase					169:174	AMP-activated protein kinase	147:174	The AMP-activated protein kinase (AMPK)	143:181	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	6	44	theme	relative	1053:1060	arg1	oxidation					1043:1051	reduced fat oxidation	1031:1051	reduced fat oxidation relative to WT	1031:1066	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	5	45	theme	Body	729:732	arg1	composition					734:744	Body composition	729:744	Body composition	729:744	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	7	46	theme	DKI	1069:1071	arg1	mice					1073:1076	DKI mice	1069:1076	DKI mice	1069:1076	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	1	47	theme	metabolism	235:244	arg1	regulator					194:202	a central regulator	184:202	a central regulator of cellular energy balance and metabolism	184:244	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	47	theme	metabolism	235:244	arg1	kinase					169:174	AMP-activated protein kinase	147:174	The AMP-activated protein kinase (AMPK)	143:181	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	6	48	theme	fat	1039:1041	arg1	oxidation					1043:1051	reduced fat oxidation	1031:1051	reduced fat oxidation relative to WT	1031:1066	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	0	49	contain	Have	57:60	arg1	Mice					0:3	Mice	0:3	Mice with Whole-Body Disruption of AMPK-Glycogen Binding	0:55	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	0	49	contain	Have	57:60	arg2	Dynamics					133:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	3	50	theme	glycogen	524:531	arg1	binding					533:539	glycogen binding	524:539	glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms	524:593	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	5	51	theme	lipid	814:818	arg1	profiles					820:827	serum hormone and lipid profiles	796:827	profiles	820:827	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	7	52	theme	reduced	1082:1088	arg1	content					1105:1111	reduced liver glycogen content	1082:1111	reduced liver glycogen content in the fed state concomitant with increased utilization	1082:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	4	53	from	effects	612:618	arg1	utilization					665:675	whole-body substrate utilization	644:675	whole-body substrate utilization	644:675	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	4	53	from	effects	612:618	arg1	dynamics					719:726	tissue glycogen dynamics	703:726	tissue glycogen dynamics	703:726	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	4	53	from	effects	612:618	arg1	homeostasis					686:696	glucose homeostasis	678:696	glucose homeostasis	678:696	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	9	54	from	effects	1455:1461	arg1	metabolism					1502:1511	whole-body metabolism	1491:1511	whole-body metabolism	1491:1511	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	9	54	from	effects	1455:1461	arg1	content					1529:1535	tissue AMPK content	1517:1535	tissue AMPK content	1517:1535	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	9	54	from	effects	1455:1461	arg1	dynamics					1550:1557	glycogen dynamics	1541:1557	glycogen dynamics	1541:1557	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	0	55	theme	Glycogen	124:131	arg1	Dynamics					133:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	3	56	from	mutations	489:497	arg1	residues					502:509	residues	502:509	residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms	502:593	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	9	57	theme	whole-body	1491:1500	arg1	metabolism					1502:1511	whole-body metabolism	1491:1511	whole-body metabolism	1491:1511	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	8	58	from	reductions	1374:1383	arg1	WT					1416:1417	WT	1416:1417	WT	1416:1417	DKI liver and skeletal muscle displayed reductions in AMPK protein content versus WT.					
34502525	8	58	from	reductions	1374:1383	arg1	content					1401:1407	AMPK protein content	1388:1407	AMPK protein content	1388:1407	DKI liver and skeletal muscle displayed reductions in AMPK protein content versus WT.					
34502525	7	59	with	concomitant	1130:1140	arg1	utilization					1157:1167	increased utilization	1147:1167	increased utilization	1147:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	3	60	theme	subunit	578:584	arg1	isoforms					586:593	subunit isoforms	578:593	subunit isoforms	578:593	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	6	61	theme	whole-body	971:980	arg1	mass					986:989	increased whole-body fat mass	961:989	increased whole-body fat mass	961:989	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	4	62	theme	glucose	678:684	arg1	homeostasis					686:696	glucose homeostasis	678:696	glucose homeostasis	678:696	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	0	63	theme	Adiposity	72:80	arg1	Dynamics					133:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	5	64	theme	tissue	834:839	arg1	glycogen					841:848	tissue glycogen and protein content	834:868	glycogen	841:848	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	4	65	theme	mutation	632:639	arg1	effects					612:618	the effects	608:618	the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics	608:726	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	7	66	theme	increased	1147:1155	arg1	utilization					1157:1167	increased utilization	1147:1167	increased utilization	1147:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	0	67	theme	Reduced	83:89	arg1	Oxidation					95:103	Reduced Fat Oxidation	83:103	Reduced Fat Oxidation	83:103	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	1	68	theme	protein	161:167	arg1	regulator					194:202	a central regulator	184:202	a central regulator of cellular energy balance and metabolism	184:244	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	68	theme	protein	161:167	arg1	AMPK					177:180	AMPK	177:180	AMPK	177:180	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	68	theme	protein	161:167	arg1	kinase					169:174	AMP-activated protein kinase	147:174	The AMP-activated protein kinase (AMPK)	143:181	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	6	69	theme	DKI	942:944	arg1	mice					946:949	DKI mice	942:949	DKI mice	942:949	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	4	70	theme	whole-body	644:653	arg1	utilization					665:675	whole-body substrate utilization	644:675	whole-body substrate utilization	644:675	We examined the effects of this DKI mutation on whole-body substrate utilization, glucose homeostasis, and tissue glycogen dynamics.					
34502525	0	71	theme	Oxidation	95:103	arg1	Dynamics					133:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics	62:140	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	5	72	theme	age-matched	909:919	arg1	mice					936:939	chow-fed male DKI and age-matched wild-type (WT) mice	887:939	mice	936:939	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	7	73	from	content	1105:1111	arg1	state					1124:1128	the fed state	1116:1128	the fed state concomitant with increased utilization	1116:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	0	74	theme	Altered	109:115	arg1	Glycogen					124:131	Altered Tissue Glycogen	109:131	Altered Tissue Glycogen	109:131	Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics.					
34502525	1	75	theme	central	186:192	arg1	regulator					194:202	a central regulator	184:202	a central regulator of cellular energy balance and metabolism	184:244	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	1	75	theme	central	186:192	arg1	kinase					169:174	AMP-activated protein kinase	147:174	The AMP-activated protein kinase (AMPK)	143:181	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	9	76	theme	tissue	1517:1522	arg1	content					1529:1535	tissue AMPK content	1517:1535	tissue AMPK content	1517:1535	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	6	77	theme	reduced	1031:1037	arg1	oxidation					1043:1051	reduced fat oxidation	1031:1051	reduced fat oxidation relative to WT	1031:1066	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	3	78	theme	knock-in	449:456	arg1	mice					464:467	AMPK β double knock-in (DKI) mice	435:467	AMPK β double knock-in (DKI) mice	435:467	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	5	79	theme	glucose	765:771	arg1	tolerance					785:793	glucose and insulin tolerance	765:793	glucose and insulin tolerance	765:793	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	9	80	theme	AMPK	1524:1527	arg1	content					1529:1535	tissue AMPK content	1517:1535	tissue AMPK content	1517:1535	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	5	81	theme	chow-fed	887:894	arg1	DKI					901:903	chow-fed male DKI and age-matched wild-type (WT) mice	887:939	DKI	901:903	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	3	82	theme	β	440:440	arg1	mice					464:467	AMPK β double knock-in (DKI) mice	435:467	AMPK β double knock-in (DKI) mice	435:467	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	5	83	theme	insulin	777:783	arg1	tolerance					785:793	glucose and insulin tolerance	765:793	glucose and insulin tolerance	765:793	Body composition, metabolic caging, glucose and insulin tolerance, serum hormone and lipid profiles, and tissue glycogen and protein content were analyzed in chow-fed male DKI and age-matched wild-type (WT) mice.					
34502525	9	84	theme	glycogen	1541:1548	arg1	dynamics					1550:1557	glycogen dynamics	1541:1557	glycogen dynamics	1541:1557	These findings identify phenotypic effects of the AMPK DKI mutation on whole-body metabolism and tissue AMPK content and glycogen dynamics.					
34502525	6	85	theme	glucose	995:1001	arg1	intolerance					1003:1013	glucose intolerance	995:1013	glucose intolerance	995:1013	DKI mice displayed increased whole-body fat mass and glucose intolerance associated with reduced fat oxidation relative to WT.					
34502525	8	86	theme	DKI	1334:1336	arg1	liver					1338:1342	DKI liver	1334:1342	DKI liver	1334:1342	DKI liver and skeletal muscle displayed reductions in AMPK protein content versus WT.					
34502525	7	87	theme	liver	1090:1094	arg1	content					1105:1111	reduced liver glycogen content	1082:1111	reduced liver glycogen content in the fed state concomitant with increased utilization	1082:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	7	88	theme	similar	1274:1280	arg1	content					1310:1316	similar glycogen-associated protein content	1274:1316	similar glycogen-associated protein content relative to WT	1274:1331	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	3	89	theme	DKI	459:461	arg1	mice					464:467	AMPK β double knock-in (DKI) mice	435:467	AMPK β double knock-in (DKI) mice	435:467	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	3	90	theme	AMPK-glycogen	412:424	arg1	binding					426:432	AMPK-glycogen binding	412:432	AMPK-glycogen binding	412:432	To chronically disrupt AMPK-glycogen binding, AMPK β double knock-in (DKI) mice were generated with mutations in residues critical for glycogen binding in both the β1 (W100A) and β2 (W98A) subunit isoforms.					
34502525	7	91	dep	fasting	1229:1235	arg1	response					1217:1224	response	1217:1224	response	1217:1224	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	2	92	theme	physiological	294:306	arg1	effects					308:314	the physiological effects	290:314	the physiological effects of disrupting AMPK-glycogen interactions	290:355	However, the physiological effects of disrupting AMPK-glycogen interactions remain incompletely understood.					
34502525	1	93	theme	energy	216:221	arg1	balance					223:229	cellular energy balance	207:229	cellular energy balance	207:229	The AMP-activated protein kinase (AMPK), a central regulator of cellular energy balance and metabolism, binds glycogen via its β subunit.					
34502525	7	94	theme	muscle	1198:1203	arg1	content					1105:1111	reduced liver glycogen content	1082:1111	reduced liver glycogen content in the fed state concomitant with increased utilization	1082:1167	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	7	94	theme	muscle	1198:1203	arg1	repletion					1176:1184	no repletion	1173:1184	no repletion of skeletal muscle glycogen	1173:1212	DKI mice had reduced liver glycogen content in the fed state concomitant with increased utilization and no repletion of skeletal muscle glycogen in response to fasting and refeeding, respectively, despite similar glycogen-associated protein content relative to WT.					
34502525	8	95	theme	AMPK	1388:1391	arg1	content					1401:1407	AMPK protein content	1388:1407	AMPK protein content	1388:1407	DKI liver and skeletal muscle displayed reductions in AMPK protein content versus WT.					
34244551	0	0	theme	young	57:61	arg1	goats					69:73	young adult goats	57:73	young adult goats	57:73	A pilot study to assess the healing of meniscal tears in young adult goats.					
34244551	6	1	theme	lesions	854:860	arg1	load					838:841	the failure load	826:841	the failure load of healing lesions	826:860	Biomechanical testing determined the failure load of healing lesions.					
34244551	8	2	theme	non-healed	1052:1061	arg1	menisci					1063:1069	both healed and non-healed menisci	1036:1069	both healed and non-healed menisci	1036:1069	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	8	3	from	menisci	1063:1069	arg1	evidence					970:977	evidence	970:977	evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears	970:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	5	4	theme	healing	688:694	arg1	status					696:701	healing status	688:701	healing status	688:701	Histology was used to assign healing status, assess compositional elements, and associate healing status with compositional elements.					
34244551	6	5	theme	healing	846:852	arg1	lesions					854:860	healing lesions	846:860	healing lesions	846:860	Biomechanical testing determined the failure load of healing lesions.					
34244551	7	6	theme	T2	886:887	arg1	*					888:888	T2*	886:888	T2*	886:888	Adiabatic T1ρ, T2, and T2* were able to quantitatively identify different healing states.					
34244551	9	7	theme	increased	1138:1146	arg1	healing					1148:1154	increased healing	1138:1154	increased healing (as assessed histologically and on MRI)	1138:1194	Biomechanical results revealed that increased healing (as assessed histologically and on MRI) was associated with greater failure load.					
34244551	3	8	theme	healing	499:505	arg1	status					507:512	the healing status	495:512	the healing status of surgically induced meniscal tears	495:549	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	0	9	theme	adult	63:67	arg1	goats					69:73	young adult goats	57:73	young adult goats	57:73	A pilot study to assess the healing of meniscal tears in young adult goats.					
34244551	4	10	dep	both	616:619	arg1	operated					621:628	operated	621:628	operated	621:628	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	2	11	theme	meniscus	243:250	arg1	status					229:234	the healing status	217:234	the healing status of the meniscus	217:250	This impedes clinical decisions as the healing status of the meniscus cannot be accurately determined non-invasively.					
34244551	0	12	from	healing	28:34	arg1	goats					69:73	young adult goats	57:73	young adult goats	57:73	A pilot study to assess the healing of meniscal tears in young adult goats.					
34244551	8	13	theme	diminished	982:991	arg1	proteoglycans					993:1005	diminished proteoglycans	982:1005	diminished proteoglycans	982:1005	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	4	14	theme	T2	567:568	arg1	times					590:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	3	15	theme	biomechanical	463:475	arg1	testing					477:483	biomechanical testing	463:483	biomechanical testing	463:483	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	4	16	theme	relaxation	579:588	arg1	times					590:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	9	17	theme	failure	1224:1230	arg1	load					1232:1235	greater failure load	1216:1235	greater failure load	1216:1235	Biomechanical results revealed that increased healing (as assessed histologically and on MRI) was associated with greater failure load.					
34244551	4	18	theme	*	577:577	arg1	times					590:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	0	19	theme	pilot	2:6	arg1	study					8:12	A pilot study	0:12	A pilot study	0:12	A pilot study to assess the healing of meniscal tears in young adult goats.					
34244551	8	20	theme	induced	1087:1093	arg1	tears					1095:1099	surgically induced tears	1076:1099	surgically induced tears	1076:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	8	21	theme	proteoglycans	993:1005	arg1	evidence					970:977	evidence	970:977	evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears	970:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	6	22	theme	failure	830:836	arg1	load					838:841	the failure load	826:841	the failure load of healing lesions	826:860	Biomechanical testing determined the failure load of healing lesions.					
34244551	3	23	theme	model	373:377	arg1	utility					355:361	the utility	351:361	the utility of a goat model	351:377	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	8	24	from	evidence	970:977	arg1	menisci					1063:1069	both healed and non-healed menisci	1036:1069	both healed and non-healed menisci	1036:1069	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	5	25	used	used	673:676	arg2	Histology					659:667	Histology	659:667	Histology	659:667	Histology was used to assign healing status, assess compositional elements, and associate healing status with compositional elements.					
34244551	7	26	theme	Adiabatic	863:871	arg1	T1ρ					873:875	Adiabatic T1ρ	863:875	Adiabatic T1ρ	863:875	Adiabatic T1ρ, T2, and T2* were able to quantitatively identify different healing states.					
34244551	4	27	dep	menisci	642:648	arg1	both					616:619	both	616:619	both	616:619	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	4	27	dep	menisci	642:648	arg1	control					634:640	control	634:640	control	634:640	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	6	28	theme	Biomechanical	793:805	arg1	testing					807:813	Biomechanical testing	793:813	Biomechanical testing	793:813	Biomechanical testing determined the failure load of healing lesions.					
34244551	8	29	theme	vascularity	1021:1031	arg1	evidence					970:977	evidence	970:977	evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears	970:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	10	30	theme	meniscal	1305:1312	arg1	strength					1314:1321	greater meniscal strength	1297:1321	greater meniscal strength	1297:1321	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	8	31	with	proteoglycans	993:1005	arg1	tears					1095:1099	surgically induced tears	1076:1099	surgically induced tears	1076:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	3	32	theme	resonance	412:420	arg1	techniques					436:445	quantitative magnetic resonance imaging (MRI) techniques	390:445	quantitative magnetic resonance imaging (MRI) techniques	390:445	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	9	33	theme	Biomechanical	1102:1114	arg1	results					1116:1122	Biomechanical results	1102:1122	Biomechanical results	1102:1122	Biomechanical results revealed that increased healing (as assessed histologically and on MRI) was associated with greater failure load.					
34244551	8	34	theme	increased	1011:1019	arg1	vascularity					1021:1031	increased vascularity	1011:1031	increased vascularity in both healed and non-healed menisci with surgically induced tears	1011:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	1	35	theme	Meniscal	76:83	arg1	injury					115:120	a common orthopedic injury	95:120	a common orthopedic injury	95:120	Meniscal tears are a common orthopedic injury, yet their healing is difficult to assess post-operatively.					
34244551	1	35	theme	Meniscal	76:83	arg1	tears					85:89	Meniscal tears	76:89	Meniscal tears	76:89	Meniscal tears are a common orthopedic injury, yet their healing is difficult to assess post-operatively.					
34244551	10	36	theme	increased	1260:1268	arg1	healing					1270:1276	increased healing	1260:1276	increased healing	1260:1276	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	3	37	theme	imaging	422:428	arg1	resonance					412:420	quantitative magnetic resonance imaging	390:428	quantitative magnetic resonance imaging (MRI) techniques	390:445	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	3	37	theme	imaging	422:428	arg1	MRI					431:433	MRI	431:433	MRI	431:433	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	10	38	from	strength	1314:1321	arg1	MRI					1396:1398	MRI	1396:1398	MRI	1396:1398	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	4	39	theme	T2	575:576	arg1	times					590:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	3	40	theme	meniscal	536:543	arg1	tears					545:549	surgically induced meniscal tears	517:549	surgically induced meniscal tears	517:549	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	11	41	theme	quantitative	1421:1432	arg1	method					1454:1459	a viable method	1445:1459	a viable method to assess meniscal tears post-operatively	1445:1501	This indicates that quantitative MRI may be a viable method to assess meniscal tears post-operatively.					
34244551	11	41	theme	quantitative	1421:1432	arg1	MRI					1434:1436	quantitative MRI	1421:1436	quantitative MRI	1421:1436	This indicates that quantitative MRI may be a viable method to assess meniscal tears post-operatively.					
34244551	3	42	theme	quantitative	390:401	arg1	resonance					412:420	quantitative magnetic resonance imaging	390:428	quantitative magnetic resonance imaging (MRI) techniques	390:445	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	3	42	theme	quantitative	390:401	arg1	MRI					431:433	MRI	431:433	MRI	431:433	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	3	43	theme	goat	368:371	arg1	model					373:377	a goat model	366:377	a goat model	366:377	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	10	44	theme	contralateral	1369:1381	arg1	controls					1383:1390	contralateral controls	1369:1390	contralateral controls	1369:1390	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	3	45	theme	tears	545:549	arg1	status					507:512	the healing status	495:512	the healing status of surgically induced meniscal tears	495:549	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	7	46	theme	healing	937:943	arg1	states					945:950	different healing states	927:950	different healing states	927:950	Adiabatic T1ρ, T2, and T2* were able to quantitatively identify different healing states.					
34244551	3	47	theme	magnetic	403:410	arg1	resonance					412:420	quantitative magnetic resonance imaging	390:428	quantitative magnetic resonance imaging (MRI) techniques	390:445	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	3	47	theme	magnetic	403:410	arg1	MRI					431:433	MRI	431:433	MRI	431:433	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	8	48	from	proteoglycans	993:1005	arg1	menisci					1063:1069	both healed and non-healed menisci	1036:1069	both healed and non-healed menisci	1036:1069	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	10	49	theme	greater	1297:1303	arg1	strength					1314:1321	greater meniscal strength	1297:1321	greater meniscal strength	1297:1321	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	5	50	theme	compositional	711:723	arg1	elements					725:732	compositional elements	711:732	compositional elements	711:732	Histology was used to assign healing status, assess compositional elements, and associate healing status with compositional elements.					
34244551	10	51	theme	relative	1357:1364	arg1	strength					1314:1321	greater meniscal strength	1297:1321	greater meniscal strength	1297:1321	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	8	52	theme	healed	1041:1046	arg1	menisci					1063:1069	both healed and non-healed menisci	1036:1069	both healed and non-healed menisci	1036:1069	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	3	53	theme	induced	528:534	arg1	tears					545:549	surgically induced meniscal tears	517:549	surgically induced meniscal tears	517:549	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34244551	10	54	from	differences	1344:1354	arg1	MRI					1396:1398	MRI	1396:1398	MRI	1396:1398	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	9	55	dep	healing	1148:1154	arg1	assessed					1160:1167	assessed	1160:1167	assessed histologically and on MRI	1160:1193	Biomechanical results revealed that increased healing (as assessed histologically and on MRI) was associated with greater failure load.					
34244551	10	56	theme	decreased	1327:1335	arg1	differences					1344:1354	decreased signal differences	1327:1354	decreased signal differences	1327:1354	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	2	57	theme	clinical	195:202	arg1	decisions					204:212	clinical decisions	195:212	clinical decisions as the healing status of the meniscus	195:250	This impedes clinical decisions as the healing status of the meniscus cannot be accurately determined non-invasively.					
34244551	4	58	theme	Adiabatic	552:560	arg1	T1ρ					562:564	Adiabatic T1ρ	552:564	Adiabatic T1ρ	552:564	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	7	59	theme	different	927:935	arg1	states					945:950	different healing states	927:950	different healing states	927:950	Adiabatic T1ρ, T2, and T2* were able to quantitatively identify different healing states.					
34244551	9	60	theme	greater	1216:1222	arg1	load					1232:1235	greater failure load	1216:1235	greater failure load	1216:1235	Biomechanical results revealed that increased healing (as assessed histologically and on MRI) was associated with greater failure load.					
34244551	2	61	theme	healing	221:227	arg1	status					229:234	the healing status	217:234	the healing status of the meniscus	217:250	This impedes clinical decisions as the healing status of the meniscus cannot be accurately determined non-invasively.					
34244551	10	62	theme	signal	1337:1342	arg1	differences					1344:1354	decreased signal differences	1327:1354	decreased signal differences	1327:1354	Our findings indicate increased healing is associated with greater meniscal strength and decreased signal differences (relative to contralateral controls) on MRI.					
34244551	1	63	theme	common	97:102	arg1	injury					115:120	a common orthopedic injury	95:120	a common orthopedic injury	95:120	Meniscal tears are a common orthopedic injury, yet their healing is difficult to assess post-operatively.					
34244551	1	63	theme	common	97:102	arg1	tears					85:89	Meniscal tears	76:89	Meniscal tears	76:89	Meniscal tears are a common orthopedic injury, yet their healing is difficult to assess post-operatively.					
34244551	8	64	with	vascularity	1021:1031	arg1	tears					1095:1099	surgically induced tears	1076:1099	surgically induced tears	1076:1099	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	0	65	theme	tears	48:52	arg1	healing					28:34	the healing	24:34	the healing of meniscal tears in young adult goats	24:73	A pilot study to assess the healing of meniscal tears in young adult goats.					
34244551	5	66	theme	compositional	769:781	arg1	elements					783:790	compositional elements	769:790	compositional elements	769:790	Histology was used to assign healing status, assess compositional elements, and associate healing status with compositional elements.					
34244551	5	67	theme	healing	749:755	arg1	status					757:762	healing status	749:762	healing status	749:762	Histology was used to assign healing status, assess compositional elements, and associate healing status with compositional elements.					
34244551	1	68	theme	orthopedic	104:113	arg1	injury					115:120	a common orthopedic injury	95:120	a common orthopedic injury	95:120	Meniscal tears are a common orthopedic injury, yet their healing is difficult to assess post-operatively.					
34244551	1	68	theme	orthopedic	104:113	arg1	tears					85:89	Meniscal tears	76:89	Meniscal tears	76:89	Meniscal tears are a common orthopedic injury, yet their healing is difficult to assess post-operatively.					
34244551	8	69	from	vascularity	1021:1031	arg1	menisci					1063:1069	both healed and non-healed menisci	1036:1069	both healed and non-healed menisci	1036:1069	Histology showed evidence of diminished proteoglycans and increased vascularity in both healed and non-healed menisci with surgically induced tears.					
34244551	0	70	theme	meniscal	39:46	arg1	tears					48:52	meniscal tears	39:52	meniscal tears	39:52	A pilot study to assess the healing of meniscal tears in young adult goats.					
34244551	4	71	theme	T1ρ	562:564	arg1	times					590:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times	552:594	Adiabatic T1ρ, T2, and T2* relaxation times were quantified for both operated and control menisci ex vivo.					
34244551	11	72	theme	viable	1447:1452	arg1	method					1454:1459	a viable method	1445:1459	a viable method to assess meniscal tears post-operatively	1445:1501	This indicates that quantitative MRI may be a viable method to assess meniscal tears post-operatively.					
34244551	11	72	theme	viable	1447:1452	arg1	MRI					1434:1436	quantitative MRI	1421:1436	quantitative MRI	1421:1436	This indicates that quantitative MRI may be a viable method to assess meniscal tears post-operatively.					
34244551	11	73	theme	meniscal	1471:1478	arg1	tears					1480:1484	meniscal tears	1471:1484	meniscal tears	1471:1484	This indicates that quantitative MRI may be a viable method to assess meniscal tears post-operatively.					
34244551	3	74	theme	study	329:333	arg1	objectives					310:319	the objectives	306:319	the objectives of this study	306:333	Thus, the objectives of this study were to explore the utility of a goat model and to use quantitative magnetic resonance imaging (MRI) techniques, histology, and biomechanical testing to assess the healing status of surgically induced meniscal tears.					
34575987	4	0	from	data	827:830	arg1	tissues					843:849	cardiac tissues	835:849	cardiac tissues	835:849	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	4	1	theme	RNA-seq	819:825	arg1	data					827:830	RNA-seq data	819:830	RNA-seq data in cardiac tissues	819:849	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	2	2	from	role	338:341	arg1	hypertension					369:380	hypertension	369:380	hypertension	369:380	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	2	3	theme	microbiota	350:359	arg1	role					338:341	the potential role	324:341	the potential role of gut microbiota (GM) in hypertension	324:380	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	7	4	theme	GM	1349:1350	arg1	composition					1334:1344	the composition	1330:1344	the composition of GM	1330:1350	In comparison to the Vehicle control, PAO could increase the microbial diversity by altering the composition of GM.					
34575987	4	5	theme	cardiac	835:841	arg1	tissues					843:849	cardiac tissues	835:849	cardiac tissues	835:849	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	9	6	theme	metabolites	1589:1599	arg1	abundance					1570:1578	the abundance	1566:1578	the abundance of major metabolites produced by Gram-negative bacteria in GM	1566:1640	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	6	7	theme	atrial	1199:1204	arg1	peptide					1218:1224	atrial natriuretic peptide	1199:1224	atrial natriuretic peptide in plasma	1199:1234	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	9	8	theme	abundance	1570:1578	arg1	influence					1553:1561	the positive influence	1540:1561	the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM	1540:1640	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	8	9	dep	Prevotella	1449:1458	arg1	bacteria					1486:1493	bacteria	1486:1493	bacteria	1486:1493	PAO could also decrease the ratio of Firmicutes to Bacteroidetes by decreasing the abundance of Prevotella and Phascolarctobacterium bacteria.					
34575987	5	10	theme	systolic	927:934	arg1	BP					936:937	systolic BP	927:937	systolic BP	927:937	We showed that the oral administration of PAO could significantly decrease systolic BP and mean arterial pressure.					
34575987	9	11	from	bacteria	1627:1634	arg1	GM					1639:1640	GM	1639:1640	GM	1639:1640	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	7	12	theme	Vehicle	1258:1264	arg1	control					1266:1272	the Vehicle control	1254:1272	the Vehicle control	1254:1272	In comparison to the Vehicle control, PAO could increase the microbial diversity by altering the composition of GM.					
34575987	0	13	theme	Hypertension	107:118	arg1	Development					92:102	the Development	88:102	the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats	88:171	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	9	14	theme	PAO	1520:1522	arg1	effect					1510:1515	The favorable effect	1496:1515	The favorable effect of PAO	1496:1522	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	3	15	theme	oligosaccharides	520:535	arg1	mechanism					543:551	the potassium alginate oligosaccharides (PAO) mechanism	497:551	the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM	497:622	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	7	16	theme	microbial	1298:1306	arg1	diversity					1308:1316	the microbial diversity	1294:1316	the microbial diversity	1294:1316	In comparison to the Vehicle control, PAO could increase the microbial diversity by altering the composition of GM.					
34575987	1	17	theme	Food-derived	174:185	arg1	oligosaccharides					187:202	Food-derived oligosaccharides	174:202	Food-derived oligosaccharides	174:202	Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood.					
34575987	9	18	theme	Gram-negative	1613:1625	arg1	bacteria					1627:1634	Gram-negative bacteria	1613:1634	Gram-negative bacteria in GM	1613:1640	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	9	19	theme	positive	1544:1551	arg1	influence					1553:1561	the positive influence	1540:1561	the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM	1540:1640	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	8	20	theme	Prevotella	1449:1458	arg1	abundance					1436:1444	the abundance	1432:1444	the abundance of Prevotella and Phascolarctobacterium bacteria	1432:1493	PAO could also decrease the ratio of Firmicutes to Bacteroidetes by decreasing the abundance of Prevotella and Phascolarctobacterium bacteria.					
34575987	0	21	theme	Alginate	10:17	arg1	Oligosaccharides					19:34	Potassium Alginate Oligosaccharides	0:34	Potassium Alginate Oligosaccharides	0:34	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	8	22	theme	Firmicutes	1390:1399	arg1	ratio					1381:1385	the ratio	1377:1385	the ratio of Firmicutes to Bacteroidetes	1377:1416	PAO could also decrease the ratio of Firmicutes to Bacteroidetes by decreasing the abundance of Prevotella and Phascolarctobacterium bacteria.					
34575987	1	23	link	Food-derived	174:185	arg1	oligosaccharides					187:202	Food-derived oligosaccharides	174:202	Food-derived oligosaccharides	174:202	Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood.					
34575987	6	24	from	concentrations	1162:1175	arg1	plasma					1229:1234	plasma	1229:1234	plasma	1229:1234	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	6	25	theme	heart	1045:1049	arg1	failure					1051:1057	heart failure	1045:1057	heart failure	1045:1057	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	0	26	theme	Potassium	0:8	arg1	Oligosaccharides					19:34	Potassium Alginate Oligosaccharides	0:34	Potassium Alginate Oligosaccharides	0:34	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	10	27	theme	disease	1773:1779	arg1	development					1743:1753	the development	1739:1753	the development of cardiovascular disease	1739:1779	We suggest that PAO caused changes in GM, and thus, they played an important role in preventing the development of cardiovascular disease.					
34575987	9	28	theme	major	1583:1587	arg1	metabolites					1589:1599	major metabolites	1583:1599	major metabolites produced by Gram-negative bacteria in GM	1583:1640	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	5	29	theme	PAO	894:896	arg1	administration					876:889	the oral administration	867:889	the oral administration of PAO	867:896	We showed that the oral administration of PAO could significantly decrease systolic BP and mean arterial pressure.					
34575987	1	30	theme	promising	209:217	arg1	potential					231:239	promising therapeutic potential	209:239	promising therapeutic potential	209:239	Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood.					
34575987	2	31	theme	GM	422:423	arg1	signature					425:433	the specific GM signature	409:433	the specific GM signature that may participate in hypertension	409:470	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	0	32	theme	Heart	124:128	arg1	Failure					130:136	Heart Failure	124:136	Heart Failure	124:136	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	6	33	theme	natriuretic	1206:1216	arg1	peptide					1218:1224	atrial natriuretic peptide	1199:1224	atrial natriuretic peptide in plasma	1199:1234	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	10	34	theme	important	1710:1718	arg1	role					1720:1723	an important role	1707:1723	an important role	1707:1723	We suggest that PAO caused changes in GM, and thus, they played an important role in preventing the development of cardiovascular disease.					
34575987	1	35	theme	therapeutic	219:229	arg1	potential					231:239	promising therapeutic potential	209:239	promising therapeutic potential	209:239	Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood.					
34575987	2	36	theme	specific	413:420	arg1	signature					425:433	the specific GM signature	409:433	the specific GM signature that may participate in hypertension	409:470	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	9	37	theme	favorable	1500:1508	arg1	effect					1510:1515	The favorable effect	1496:1515	The favorable effect of PAO	1496:1522	The favorable effect of PAO may be added to the positive influence of the abundance of major metabolites produced by Gram-negative bacteria in GM.					
34575987	6	38	theme	gene	1124:1127	arg1	expression					1129:1138	A gene expression	1122:1138	A gene expression	1122:1138	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	3	39	theme	weeks	724:728	arg1	duration					708:715	a duration	706:715	a duration of six weeks	706:728	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	10	40	theme	cardiovascular	1758:1771	arg1	disease					1773:1779	cardiovascular disease	1758:1779	cardiovascular disease	1758:1779	We suggest that PAO caused changes in GM, and thus, they played an important role in preventing the development of cardiovascular disease.					
34575987	3	41	theme	various	641:647	arg1	dosages					649:655	various dosages	641:655	various dosages of PAO in 40 spontaneously hypertensive rats	641:700	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	3	42	theme	microbial	581:589	arg1	changes					601:607	specific microbial signature changes	572:607	specific microbial signature changes	572:607	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	6	43	theme	protective	1012:1021	arg1	effects					1023:1029	the protective effects	1008:1029	the protective effects of developing heart failure	1008:1057	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	0	44	theme	Gut	42:44	arg1	Microbiota					46:55	Gut Microbiota	42:55	Gut Microbiota	42:55	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	0	45	theme	Failure	130:136	arg1	Development					92:102	the Development	88:102	the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats	88:171	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	3	46	theme	signature	591:599	arg1	changes					601:607	specific microbial signature changes	572:607	specific microbial signature changes	572:607	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	0	47	theme	Hypertensive	155:166	arg1	Rats					168:171	Spontaneously Hypertensive Rats	141:171	Spontaneously Hypertensive Rats	141:171	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	4	48	theme	cecum	791:795	arg1	content					797:803	the cecum content	787:803	the cecum content	787:803	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	2	49	theme	gut	346:348	arg1	GM					362:363	GM	362:363	GM	362:363	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	2	49	theme	gut	346:348	arg1	microbiota					350:359	gut microbiota	346:359	gut microbiota (GM)	346:364	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	3	50	theme	PAO	660:662	arg1	dosages					649:655	various dosages	641:655	various dosages of PAO in 40 spontaneously hypertensive rats	641:700	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	3	51	theme	specific	572:579	arg1	changes					601:607	specific microbial signature changes	572:607	specific microbial signature changes	572:607	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	6	52	dep	accompanied	1064:1074	arg1	down-regulating					1079:1093	down-regulating	1079:1093	down-regulating of the Natriuretic Peptide A gene expression	1079:1138	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	6	52	dep	accompanied	1064:1074	arg1	decreasing					1147:1156	decreasing	1147:1156	decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma	1147:1234	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	6	53	from	peptide	1218:1224	arg1	plasma					1229:1234	plasma	1229:1234	plasma	1229:1234	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	6	54	theme	angiotensin	1180:1190	arg1	peptide					1218:1224	atrial natriuretic peptide	1199:1224	atrial natriuretic peptide in plasma	1199:1234	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	6	54	theme	angiotensin	1180:1190	arg1	concentrations					1162:1175	the concentrations	1158:1175	the concentrations of angiotensin II	1158:1193	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	5	55	theme	mean	943:946	arg1	pressure					957:964	mean arterial pressure	943:964	mean arterial pressure	943:964	We showed that the oral administration of PAO could significantly decrease systolic BP and mean arterial pressure.					
34575987	4	56	theme	16S	761:763	arg1	gene					779:782	the 16S ribosomal DNA gene	757:782	the 16S ribosomal DNA gene in the cecum content	757:803	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	4	57	from	gene	779:782	arg1	content					797:803	the cecum content	787:803	the cecum content	787:803	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	5	58	theme	oral	871:874	arg1	administration					876:889	the oral administration	867:889	the oral administration of PAO	867:896	We showed that the oral administration of PAO could significantly decrease systolic BP and mean arterial pressure.					
34575987	10	59	from	changes	1670:1676	arg1	GM					1681:1682	GM	1681:1682	GM	1681:1682	We suggest that PAO caused changes in GM, and thus, they played an important role in preventing the development of cardiovascular disease.					
34575987	1	60	theme	blood	253:257	arg1	BP					269:270	BP	269:270	BP	269:270	Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood.					
34575987	1	60	theme	blood	253:257	arg1	pressure					259:266	blood pressure	253:266	blood pressure (BP)	253:271	Food-derived oligosaccharides show promising therapeutic potential in lowering blood pressure (BP), but the mechanism is poorly understood.					
34575987	0	61	from	Development	92:102	arg1	Rats					168:171	Spontaneously Hypertensive Rats	141:171	Spontaneously Hypertensive Rats	141:171	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	5	62	theme	arterial	948:955	arg1	pressure					957:964	mean arterial pressure	943:964	mean arterial pressure	943:964	We showed that the oral administration of PAO could significantly decrease systolic BP and mean arterial pressure.					
34575987	2	63	theme	potential	328:336	arg1	role					338:341	the potential role	324:341	the potential role of gut microbiota (GM) in hypertension	324:380	Recently, the potential role of gut microbiota (GM) in hypertension has been investigated, but the specific GM signature that may participate in hypertension remains unclear.					
34575987	6	64	theme	Natriuretic	1102:1112	arg1	Peptide					1114:1120	the Natriuretic Peptide	1098:1120	the Natriuretic Peptide	1098:1120	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
34575987	3	65	from	dosages	649:655	arg1	rats					697:700	40 spontaneously hypertensive rats	667:700	40 spontaneously hypertensive rats	667:700	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	8	66	theme	Phascolarctobacterium	1464:1484	arg1	abundance					1436:1444	the abundance	1432:1444	the abundance of Prevotella and Phascolarctobacterium bacteria	1432:1493	PAO could also decrease the ratio of Firmicutes to Bacteroidetes by decreasing the abundance of Prevotella and Phascolarctobacterium bacteria.					
34575987	4	67	theme	DNA	775:777	arg1	gene					779:782	the 16S ribosomal DNA gene	757:782	the 16S ribosomal DNA gene in the cecum content	757:803	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	3	68	theme	potassium	501:509	arg1	PAO					538:540	PAO	538:540	PAO	538:540	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	3	68	theme	potassium	501:509	arg1	oligosaccharides					520:535	potassium alginate oligosaccharides	501:535	the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM	497:622	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	3	69	theme	hypertensive	684:695	arg1	rats					697:700	40 spontaneously hypertensive rats	667:700	40 spontaneously hypertensive rats	667:700	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	4	70	theme	ribosomal	765:773	arg1	gene					779:782	the 16S ribosomal DNA gene	757:782	the 16S ribosomal DNA gene in the cecum content	757:803	We analyzed BP, sequenced the 16S ribosomal DNA gene in the cecum content, and gathered RNA-seq data in cardiac tissues.					
34575987	0	71	contain	Have	62:65	arg1	Oligosaccharides					19:34	Potassium Alginate Oligosaccharides	0:34	Potassium Alginate Oligosaccharides	0:34	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	0	71	contain	Have	62:65	arg2	Potential					67:75	Potential	67:75	Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats	67:171	Potassium Alginate Oligosaccharides Alter Gut Microbiota, and Have Potential to Prevent the Development of Hypertension and Heart Failure in Spontaneously Hypertensive Rats.					
34575987	3	72	theme	alginate	511:518	arg1	PAO					538:540	PAO	538:540	PAO	538:540	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	3	72	theme	alginate	511:518	arg1	oligosaccharides					520:535	potassium alginate oligosaccharides	501:535	the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM	497:622	To test the potassium alginate oligosaccharides (PAO) mechanism in lowering BP and specific microbial signature changes in altering GM, we administered various dosages of PAO in 40 spontaneously hypertensive rats for a duration of six weeks.					
34575987	6	73	theme	Transcriptome	967:979	arg1	analyses					981:988	Transcriptome analyses	967:988	Transcriptome analyses	967:988	Transcriptome analyses demonstrated that the protective effects of developing heart failure were accompanied by down-regulating of the Natriuretic Peptide A gene expression and by decreasing the concentrations of angiotensin II and atrial natriuretic peptide in plasma.					
32888361	9	0	theme	nutrition	1794:1802	arg1	composition					1804:1814	their nutrition composition	1788:1814	their nutrition composition	1788:1814	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	1	1	theme	ultrasound	225:234	arg1	[UAE					256:259	ultrasound assisted extraction [UAE	225:259	ultrasound assisted extraction [UAE	225:259	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	2	theme	extraction	245:254	arg1	[UAE					256:259	ultrasound assisted extraction [UAE	225:259	ultrasound assisted extraction [UAE	225:259	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	3	3	theme	monosaccharide	898:911	arg1	composition					913:923	the identical monosaccharide composition	884:923	the identical monosaccharide composition	884:923	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	2	4	theme	higher	749:754	arg1	yield					756:760	higher yield	749:760	higher yield (8.96%)	749:768	The HWE achieved higher yield (8.96%) than UAE (7.84%) followed by MAE (7.01%) and AAE (6.44%).					
32888361	2	4	theme	higher	749:754	arg1	%					767:767	8.96%	763:767	8.96%	763:767	The HWE achieved higher yield (8.96%) than UAE (7.84%) followed by MAE (7.01%) and AAE (6.44%).					
32888361	1	5	theme	monosaccharide	417:430	arg1	composition					432:442	monosaccharide composition	417:442	monosaccharide composition	417:442	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	6	from	2,2'-azino-bis	631:644	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	7	theme	water	167:171	arg1	extraction					173:182	hot water extraction [HWE	163:187	hot water extraction [HWE	163:187	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	3	8	theme	slight	930:935	arg1	difference					937:946	slight difference	930:946	slight difference	930:946	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	1	9	theme	3-ethylbenzothiazoline-6-sulphonic	646:679	arg1	[ABTS					686:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	9	theme	3-ethylbenzothiazoline-6-sulphonic	646:679	arg1	2,2'-azino-bis					631:644	2,2'-azino-bis	631:644	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS]	631:691	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	0	10	theme	flaxseed	112:119	arg1	gum					121:123	flaxseed gum	112:123	flaxseed gum	112:123	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	5	11	theme	power	1231:1235	arg1	assay					1263:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay	1184:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG	1184:1309	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	8	12	theme	extraction	1659:1668	arg1	method					1670:1675	appropriate extraction method	1647:1675	appropriate extraction method	1647:1675	Thus, this study could help in selection of appropriate extraction method for FSG.					
32888361	1	13	dep	characterization	399:414	arg1	composition					432:442	monosaccharide composition	417:442	monosaccharide composition	417:442	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	13	dep	characterization	399:414	arg1	distribution					462:473	molecular weight distribution	445:473	molecular weight distribution	445:473	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	13	dep	characterization	399:414	arg1	microstructure					480:493	microstructure	480:493	microstructure	480:493	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	5	14	theme	significant	1143:1153	arg1	ability					1173:1179	significant higher scavenging ability	1143:1179	significant higher scavenging ability	1143:1179	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	5	15	theme	radical	1213:1219	arg1	assay					1263:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay	1184:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG	1184:1309	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	6	16	theme	special	1475:1481	arg1	equipment					1483:1491	no special equipment	1472:1491	no special equipment	1472:1491	Overall results showed that UAE was favorable to the purity of FSG, whereas HWE was more advantageous to improve the extraction yield and facile as it requires no special equipment.					
32888361	7	17	theme	PRACTICAL	1494:1502	arg1	APPLICATION					1504:1514	PRACTICAL APPLICATION	1494:1514	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.	1494:1601	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.					
32888361	9	18	used	used	1864:1867	arg2	it					1854:1855	it	1854:1855	it	1854:1855	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	9	18	used	used	1864:1867	arg2	agent					1929:1933	a natural antioxidant agent	1907:1933	a natural antioxidant agent	1907:1933	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	9	19	theme	functional	1888:1897	arg1	food					1899:1902	functional food	1888:1902	functional food	1888:1902	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	5	20	theme	reducing	1222:1229	arg1	power					1231:1235	reducing power	1222:1235	reducing power	1222:1235	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	1	21	from	purity	380:385	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	22	theme	2,2-diphenyl-1-picrylhydrazyl	593:621	arg1	[DPPH					623:627	2,2-diphenyl-1-picrylhydrazyl [DPPH	593:627	2,2-diphenyl-1-picrylhydrazyl [DPPH	593:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	0	23	theme	antioxidant	66:76	arg1	activities					78:87	antioxidant activities	66:87	antioxidant activities	66:87	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	24	from	Influence	0:8	arg1	microstructure					94:107	microstructure	94:107	microstructure of flaxseed gum	94:123	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	24	from	Influence	0:8	arg1	purity					58:63	purity	58:63	purity	58:63	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	24	from	Influence	0:8	arg1	activities					78:87	antioxidant activities	66:87	antioxidant activities	66:87	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	24	from	Influence	0:8	arg1	recovery					48:55	recovery	48:55	recovery	48:55	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	9	25	theme	natural	1909:1915	arg1	agent					1929:1933	a natural antioxidant agent	1907:1933	a natural antioxidant agent	1907:1933	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	9	25	theme	natural	1909:1915	arg1	it					1854:1855	it	1854:1855	it	1854:1855	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	5	26	theme	ABTS	1203:1206	arg1	radical					1213:1219	ABTS free radical	1203:1219	ABTS free radical	1203:1219	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	7	27	dep	APPLICATION	1504:1514	arg1	affect					1545:1550	affect	1545:1550	affect the recovery and purity of the extracted compound	1545:1600	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.					
32888361	3	28	located	observed	952:959	arg1	content					968:974	the content	964:974	the content	964:974	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	3	28	located	observed	952:959	arg2	difference					937:946	slight difference	930:946	slight difference	930:946	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	1	29	theme	gum	331:333	arg1	extraction					335:344	flaxseed gum extraction	322:344	flaxseed gum extraction	322:344	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	3	30	theme	molecular	989:997	arg1	ratios					1006:1011	the molecular weight ratios	985:1011	the molecular weight ratios	985:1011	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	7	31	theme	extracted	1583:1591	arg1	compound					1593:1600	the extracted compound	1579:1600	the extracted compound	1579:1600	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.					
32888361	1	32	from	extraction	335:344	arg1	2,2'-azino-bis					631:644	2,2'-azino-bis	631:644	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS]	631:691	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	32	from	extraction	335:344	arg1	characterization					399:414	structural characterization	388:414	structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope)	388:530	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	32	from	extraction	335:344	arg1	activity					549:556	antioxidant activity	537:556	antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH]	537:628	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	32	from	extraction	335:344	arg1	power					707:711	reducing power	698:711	reducing power	698:711	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	32	from	extraction	335:344	arg1	yield					373:377	the yield	369:377	the yield	369:377	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	32	from	extraction	335:344	arg1	purity					380:385	purity	380:385	purity	380:385	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	32	from	extraction	335:344	arg1	[ABTS					686:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	0	33	theme	different	13:21	arg1	techniques					34:43	different extraction techniques	13:43	different extraction techniques	13:43	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	3	34	theme	significant	1023:1033	arg1	difference					1035:1044	significant difference	1023:1044	significant difference	1023:1044	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	9	35	theme	potential	1727:1735	arg1	application					1748:1758	potential healthcare application	1727:1758	potential healthcare application in food industry	1727:1775	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	5	36	theme	β-carotene	1242:1251	arg1	bleaching					1253:1261	β-carotene bleaching	1242:1261	β-carotene bleaching	1242:1261	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	0	37	theme	techniques	34:43	arg1	Influence					0:8	Influence	0:8	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.	0:124	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	1	38	theme	microwave	267:275	arg1	[MAE					297:300	microwave assisted extraction [MAE	267:300	microwave assisted extraction [MAE	267:300	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	39	dep	is	159:160	arg1	extraction					173:182	hot water extraction [HWE	163:187	hot water extraction [HWE	163:187	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	40	theme	extraction	286:295	arg1	[MAE					297:300	microwave assisted extraction [MAE	267:300	microwave assisted extraction [MAE	267:300	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	41	from	power	707:711	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	3	42	theme	flaxseed	847:854	arg1	samples					866:872	the four flaxseed gum (FSG) samples	838:872	the four flaxseed gum (FSG) samples	838:872	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	9	43	theme	food	1763:1766	arg1	industry					1768:1775	food industry	1763:1775	food industry	1763:1775	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	1	44	theme	alkaline-acidic	191:205	arg1	[AAE					218:221	alkaline-acidic extraction [AAE	191:221	alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE]	191:260	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	2	45	dep	achieved	740:747	arg1	followed					787:794	followed	787:794	followed by MAE (7.01%) and AAE (6.44%)	787:825	The HWE achieved higher yield (8.96%) than UAE (7.84%) followed by MAE (7.01%) and AAE (6.44%).					
32888361	9	46	from	application	1748:1758	arg1	industry					1768:1775	food industry	1763:1775	food industry	1763:1775	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	1	47	theme	electron	511:518	arg1	microscope					520:529	transmission electron microscope	498:529	transmission electron microscope	498:529	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	6	48	theme	FSG	1375:1377	arg1	purity					1365:1370	the purity	1361:1370	the purity of FSG	1361:1377	Overall results showed that UAE was favorable to the purity of FSG, whereas HWE was more advantageous to improve the extraction yield and facile as it requires no special equipment.					
32888361	1	49	theme	structural	388:397	arg1	characterization					399:414	structural characterization	388:414	structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope)	388:530	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	50	theme	assisted	236:243	arg1	[UAE					256:259	ultrasound assisted extraction [UAE	225:259	ultrasound assisted extraction [UAE	225:259	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	3	51	theme	identical	888:896	arg1	composition					913:923	the identical monosaccharide composition	884:923	the identical monosaccharide composition	884:923	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	9	52	theme	antioxidant	1820:1830	arg1	activities					1832:1841	antioxidant activities	1820:1841	antioxidant activities	1820:1841	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	5	53	theme	radical	1194:1200	arg1	assay					1263:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay	1184:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG	1184:1309	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	1	54	from	yield	373:377	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	6	55	theme	extraction	1429:1438	arg1	yield					1440:1444	the extraction yield	1425:1444	the extraction yield	1425:1444	Overall results showed that UAE was favorable to the purity of FSG, whereas HWE was more advantageous to improve the extraction yield and facile as it requires no special equipment.					
32888361	1	56	theme	hot	163:165	arg1	extraction					173:182	hot water extraction [HWE	163:187	hot water extraction [HWE	163:187	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	0	57	theme	gum	121:123	arg1	microstructure					94:107	microstructure	94:107	microstructure of flaxseed gum	94:123	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	57	theme	gum	121:123	arg1	purity					58:63	purity	58:63	purity	58:63	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	57	theme	gum	121:123	arg1	activities					78:87	antioxidant activities	66:87	antioxidant activities	66:87	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	0	57	theme	gum	121:123	arg1	recovery					48:55	recovery	48:55	recovery	48:55	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	1	58	theme	molecular	445:453	arg1	distribution					462:473	molecular weight distribution	445:473	molecular weight distribution	445:473	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	59	dep	[AAE	218:221	arg1	[UAE					256:259	ultrasound assisted extraction [UAE	225:259	ultrasound assisted extraction [UAE	225:259	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	60	theme	acid	681:684	arg1	[ABTS					686:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	60	theme	acid	681:684	arg1	2,2'-azino-bis					631:644	2,2'-azino-bis	631:644	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS]	631:691	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	9	61	contain	possesses	1717:1725	arg1	FSG					1713:1715	FSG	1713:1715	FSG	1713:1715	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	9	61	contain	possesses	1717:1725	arg2	application					1748:1758	potential healthcare application	1727:1758	potential healthcare application in food industry	1727:1775	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	1	62	theme	ability	582:588	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	7	63	theme	Extraction	1517:1526	arg1	methods					1528:1534	Extraction methods	1517:1534	Extraction methods	1517:1534	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.					
32888361	4	64	theme	antioxidant	1108:1118	arg1	assays					1120:1125	all antioxidant assays	1104:1125	all antioxidant assays	1104:1125	All samples displayed concentration-dependent manner for all antioxidant assays.					
32888361	8	65	theme	appropriate	1647:1657	arg1	method					1670:1675	appropriate extraction method	1647:1675	appropriate extraction method	1647:1675	Thus, this study could help in selection of appropriate extraction method for FSG.					
32888361	5	66	dep	significant	1143:1153	arg1	higher					1155:1160	higher	1155:1160	higher	1155:1160	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	1	67	from	activity	549:556	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	5	68	theme	free	1208:1211	arg1	radical					1213:1219	ABTS free radical	1203:1219	ABTS free radical	1203:1219	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	8	69	theme	method	1670:1675	arg1	selection					1634:1642	selection	1634:1642	selection of appropriate extraction method for FSG	1634:1683	Thus, this study could help in selection of appropriate extraction method for FSG.					
32888361	4	70	theme	concentration-dependent	1069:1091	arg1	manner					1093:1098	concentration-dependent manner	1069:1098	concentration-dependent manner for all antioxidant assays	1069:1125	All samples displayed concentration-dependent manner for all antioxidant assays.					
32888361	1	71	theme	scavenging	571:580	arg1	ability					582:588	scavenging ability	571:588	scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	571:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	6	72	theme	Overall	1312:1318	arg1	results					1320:1326	Overall results	1312:1326	Overall results	1312:1326	Overall results showed that UAE was favorable to the purity of FSG, whereas HWE was more advantageous to improve the extraction yield and facile as it requires no special equipment.					
32888361	1	73	theme	extraction	131:140	arg1	techniques					142:151	Four extraction techniques	126:151	Four extraction techniques (that is, hot water extraction [HWE]	126:188	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	73	theme	extraction	131:140	arg1	is					159:160	is	159:160	is	159:160	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	9	74	theme	food	1899:1902	arg1	formulation					1873:1883	formulation	1873:1883	formulation of functional food	1873:1902	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	5	75	theme	scavenging	1162:1171	arg1	ability					1173:1179	significant higher scavenging ability	1143:1179	significant higher scavenging ability	1143:1179	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	5	76	theme	free	1189:1192	arg1	radical					1194:1200	DPPH free radical	1184:1200	DPPH free radical	1184:1200	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	1	77	theme	[DPPH	623:627	arg1	ability					582:588	scavenging ability	571:588	scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	571:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	2,2'-azino-bis					631:644	2,2'-azino-bis	631:644	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS]	631:691	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	characterization					399:414	structural characterization	388:414	structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope)	388:530	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	activity					549:556	antioxidant activity	537:556	antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH]	537:628	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	power					707:711	reducing power	698:711	reducing power	698:711	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	yield					373:377	the yield	369:377	the yield	369:377	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	purity					380:385	purity	380:385	purity	380:385	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	78	from	influence	356:364	arg1	[ABTS					686:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	3-ethylbenzothiazoline-6-sulphonic acid [ABTS	646:690	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	9	79	theme	antioxidant	1917:1927	arg1	agent					1929:1933	a natural antioxidant agent	1907:1933	a natural antioxidant agent	1907:1933	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	9	79	theme	antioxidant	1917:1927	arg1	it					1854:1855	it	1854:1855	it	1854:1855	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	5	80	theme	bleaching	1253:1261	arg1	assay					1263:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay	1184:1267	DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG	1184:1309	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32888361	1	81	theme	flaxseed	322:329	arg1	extraction					335:344	flaxseed gum extraction	322:344	flaxseed gum extraction	322:344	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	7	82	theme	compound	1593:1600	arg1	purity					1569:1574	purity	1569:1574	purity	1569:1574	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.					
32888361	7	82	theme	compound	1593:1600	arg1	recovery					1556:1563	recovery	1556:1563	recovery	1556:1563	PRACTICAL APPLICATION: Extraction methods directly affect the recovery and purity of the extracted compound.					
32888361	1	83	theme	antioxidant	537:547	arg1	activity					549:556	antioxidant activity	537:556	antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH]	537:628	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	0	84	theme	extraction	23:32	arg1	techniques					34:43	different extraction techniques	13:43	different extraction techniques	13:43	Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum.					
32888361	3	85	theme	weight	999:1004	arg1	ratios					1006:1011	the molecular weight ratios	985:1011	the molecular weight ratios	985:1011	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	9	86	theme	healthcare	1737:1746	arg1	application					1748:1758	potential healthcare application	1727:1758	potential healthcare application in food industry	1727:1775	The results suggested that FSG possesses potential healthcare application in food industry because of their nutrition composition and antioxidant activities, and thus, it can be used for formulation of functional food as a natural antioxidant agent.					
32888361	1	87	theme	weight	455:460	arg1	distribution					462:473	molecular weight distribution	445:473	molecular weight distribution	445:473	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	88	theme	assisted	277:284	arg1	[MAE					297:300	microwave assisted extraction [MAE	267:300	microwave assisted extraction [MAE	267:300	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	3	89	theme	FSG	861:863	arg1	samples					866:872	the four flaxseed gum (FSG) samples	838:872	the four flaxseed gum (FSG) samples	838:872	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	3	90	theme	gum	856:858	arg1	samples					866:872	the four flaxseed gum (FSG) samples	838:872	the four flaxseed gum (FSG) samples	838:872	Moreover, the four flaxseed gum (FSG) samples exhibited the identical monosaccharide composition, but slight difference was observed in the content, whereas the molecular weight ratios exhibited significant difference.					
32888361	1	91	theme	extraction	207:216	arg1	[AAE					218:221	alkaline-acidic extraction [AAE	191:221	alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE]	191:260	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	92	from	characterization	399:414	arg1	terms					562:566	terms	562:566	terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH	562:627	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	93	theme	reducing	698:705	arg1	power					707:711	reducing power	698:711	reducing power	698:711	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	1	94	theme	transmission	498:509	arg1	microscope					520:529	transmission electron microscope	498:529	transmission electron microscope	498:529	Four extraction techniques (that is, hot water extraction [HWE], alkaline-acidic extraction [AAE], ultrasound assisted extraction [UAE], and microwave assisted extraction [MAE]) were compared for flaxseed gum extraction and their influence on the yield, purity, structural characterization (monosaccharide composition, molecular weight distribution, and microstructure by transmission electron microscope), and antioxidant activity (in terms of scavenging ability of 2,2-diphenyl-1-picrylhydrazyl [DPPH], 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and reducing power) was investigated.					
32888361	5	95	theme	DPPH	1184:1187	arg1	radical					1194:1200	DPPH free radical	1184:1200	DPPH free radical	1184:1200	UAE-FSG showed significant higher scavenging ability on DPPH free radical, ABTS free radical, reducing power, and β-carotene bleaching assay followed by HWE-FSG, MAE-FSG, and AAE-FSG.					
32509874	0	0	theme	Diet-Induced	111:122	arg1	Obesity					124:130	High-Fat Diet-Induced Obesity	102:130	High-Fat Diet-Induced Obesity	102:130	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	9	1	theme	gut	1231:1233	arg1	composition					1246:1256	the gut microbiota composition	1227:1256	the gut microbiota composition	1227:1256	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	4	2	theme	diet-induced	704:715	arg1	obesity					717:723	HF (60 kcal %) diet-induced obesity	689:723	HF (60 kcal %) diet-induced obesity	689:723	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	4	3	theme	prebiotic	611:619	arg1	xylooligosaccharides					621:640	prebiotic xylooligosaccharides	611:640	prebiotic xylooligosaccharides (XOS)	611:646	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	4	3	theme	prebiotic	611:619	arg1	XOS					643:645	XOS	643:645	XOS	643:645	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	0	4	theme	High-Fat	102:109	arg1	Obesity					124:130	High-Fat Diet-Induced Obesity	102:130	High-Fat Diet-Induced Obesity	102:130	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	9	5	theme	microbiota	1235:1244	arg1	composition					1246:1256	the gut microbiota composition	1227:1256	the gut microbiota composition	1227:1256	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	1	6	theme	diverse	266:272	arg1	diseases					287:294	diverse inflammatory diseases	266:294	diverse inflammatory diseases	266:294	Gut dysbiosis induced by high-fat diet (HFD) may result in low-grade inflammation leading to diverse inflammatory diseases.					
32509874	11	7	theme	prebiotic	1591:1599	arg1	supplementation					1605:1619	prebiotic XOS supplementation	1591:1619	prebiotic XOS supplementation	1591:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	10	8	theme	XOS	1400:1402	arg1	supplements					1369:1379	supplements	1369:1379	supplements of combined BL and XOS	1369:1402	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	5	9	theme	XOS	837:839	arg1	supplementation					841:855	XOS supplementation	837:855	XOS supplementation	837:855	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	1	10	theme	inflammatory	274:285	arg1	diseases					287:294	diverse inflammatory diseases	266:294	diverse inflammatory diseases	266:294	Gut dysbiosis induced by high-fat diet (HFD) may result in low-grade inflammation leading to diverse inflammatory diseases.					
32509874	5	11	theme	male	741:744	arg1	rats					766:769	male Sprague Dawley (SD) rats	741:769	male Sprague Dawley (SD) rats	741:769	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	11	12	theme	positive	1627:1634	arg1	effect					1636:1641	a positive effect	1625:1641	a positive effect on HFD-induced obesity in rats	1625:1672	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	2	13	theme	beneficial	301:310	arg1	effects					312:318	The beneficial effects	297:318	The beneficial effects of probiotics and prebiotics on obesity	297:358	The beneficial effects of probiotics and prebiotics on obesity have been reported previously.					
32509874	10	14	theme	BL	1393:1394	arg1	supplements					1369:1379	supplements	1369:1379	supplements of combined BL and XOS	1369:1402	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	8	15	theme	body	1122:1125	arg1	gain					1134:1137	body weight gain	1122:1137	body weight gain	1122:1137	In contrast, combined BL and XOS supplementation inhibited body weight gain and returned lipid metabolism to normal.					
32509874	11	16	from	obesity	1658:1664	arg1	rats					1669:1672	rats	1669:1672	rats	1669:1672	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	7	17	theme	disordered	1034:1043	arg1	metabolism					1051:1060	disordered lipid metabolism	1034:1060	disordered lipid metabolism	1034:1060	Rats fed an HFD exhibited increased body weight and disordered lipid metabolism.					
32509874	11	18	contain	has	1621:1623	arg2	effect					1636:1641	a positive effect	1625:1641	a positive effect on HFD-induced obesity in rats	1625:1672	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	11	18	contain	has	1621:1623	arg1	modulation					1526:1535	modulation	1526:1535	modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation	1526:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	11	19	theme	XOS	1601:1603	arg1	supplementation					1605:1619	prebiotic XOS supplementation	1591:1619	prebiotic XOS supplementation	1591:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	0	20	theme	Zhengchangsheng®	52:67	arg1	Supplements					5:15	Oral Supplements	0:15	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides	0:92	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	9	21	theme	specific	1272:1279	arg1	Prevotellaceae					1298:1311	Prevotellaceae	1298:1311	Prevotellaceae	1298:1311	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	9	21	theme	specific	1272:1279	arg1	Ruminococcaceae					1339:1353	Ruminococcaceae	1339:1353	Ruminococcaceae	1339:1353	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	9	21	theme	specific	1272:1279	arg1	Desulfovibrionaceae					1314:1332	Desulfovibrionaceae	1314:1332	Desulfovibrionaceae	1314:1332	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	9	21	theme	specific	1272:1279	arg1	bacteria					1281:1288	specific bacteria	1272:1288	specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae	1272:1353	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	7	22	theme	lipid	1045:1049	arg1	metabolism					1051:1060	disordered lipid metabolism	1034:1060	disordered lipid metabolism	1034:1060	Rats fed an HFD exhibited increased body weight and disordered lipid metabolism.					
32509874	7	23	theme	body	1018:1021	arg1	weight					1023:1028	increased body weight	1008:1028	increased body weight	1008:1028	Rats fed an HFD exhibited increased body weight and disordered lipid metabolism.					
32509874	8	24	theme	weight	1127:1132	arg1	gain					1134:1137	body weight gain	1122:1137	body weight gain	1122:1137	In contrast, combined BL and XOS supplementation inhibited body weight gain and returned lipid metabolism to normal.					
32509874	1	25	theme	high-fat	198:205	arg1	diet					207:210	high-fat diet	198:210	high-fat diet (HFD)	198:216	Gut dysbiosis induced by high-fat diet (HFD) may result in low-grade inflammation leading to diverse inflammatory diseases.					
32509874	1	25	theme	high-fat	198:205	arg1	HFD					213:215	HFD	213:215	HFD	213:215	Gut dysbiosis induced by high-fat diet (HFD) may result in low-grade inflammation leading to diverse inflammatory diseases.					
32509874	5	26	theme	fat	789:791	arg1	diet					793:796	a normal fat diet	780:796	a normal fat diet (CON)	780:802	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	5	26	theme	fat	789:791	arg1	CON					799:801	CON	799:801	CON	799:801	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	5	27	theme	rats	766:769	arg1	rats					766:769	male Sprague Dawley (SD) rats	741:769	male Sprague Dawley (SD) rats	741:769	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	5	27	theme	rats	766:769	arg1	groups					731:736	Five groups	726:736	Five groups of male Sprague Dawley (SD) rats	726:769	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	10	28	theme	serum	1426:1430	arg1	related					1467:1473	related	1467:1473	related	1467:1473	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	10	28	theme	serum	1426:1430	arg1	level					1436:1440	the serum LPS level	1422:1440	the serum LPS level	1422:1440	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	10	29	theme	combined	1384:1391	arg1	BL					1393:1394	combined BL	1384:1394	combined BL	1384:1394	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	2	30	theme	prebiotics	338:347	arg1	effects					312:318	The beneficial effects	297:318	The beneficial effects of probiotics and prebiotics on obesity	297:358	The beneficial effects of probiotics and prebiotics on obesity have been reported previously.					
32509874	0	31	theme	Oral	0:3	arg1	Supplements					5:15	Oral Supplements	0:15	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides	0:92	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	3	32	theme	underlying	449:458	arg1	mechanisms					460:469	the underlying mechanisms	445:469	the underlying mechanisms	445:469	However, their benefits in promoting human health and the underlying mechanisms still need to be further characterized.					
32509874	0	33	theme	Combined	20:27	arg1	Zhengchangsheng®					52:67	Combined Bacillus licheniformis Zhengchangsheng®	20:67	Combined Bacillus licheniformis Zhengchangsheng®	20:67	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	6	34	theme	microbiota	915:924	arg1	composition					926:936	microbiota composition	915:936	microbiota composition	915:936	Lipid profiles, inflammatory biomarkers, and microbiota composition were analyzed at the end of the experiment.					
32509874	7	35	theme	increased	1008:1016	arg1	weight					1023:1028	increased body weight	1008:1028	increased body weight	1008:1028	Rats fed an HFD exhibited increased body weight and disordered lipid metabolism.					
32509874	2	36	theme	probiotics	323:332	arg1	effects					312:318	The beneficial effects	297:318	The beneficial effects of probiotics and prebiotics on obesity	297:358	The beneficial effects of probiotics and prebiotics on obesity have been reported previously.					
32509874	11	37	from	effect	1636:1641	arg1	obesity					1658:1664	HFD-induced obesity	1646:1664	HFD-induced obesity in rats	1646:1672	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	0	38	theme	Gut	149:151	arg1	Microbiota					153:162	the Gut Microbiota	145:162	the Gut Microbiota in Rats	145:170	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	0	39	theme	licheniformis	38:50	arg1	Zhengchangsheng®					52:67	Combined Bacillus licheniformis Zhengchangsheng®	20:67	Combined Bacillus licheniformis Zhengchangsheng®	20:67	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	4	40	theme	HF	689:690	arg1	obesity					717:723	HF (60 kcal %) diet-induced obesity	689:723	HF (60 kcal %) diet-induced obesity	689:723	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	4	41	theme	rat	674:676	arg1	model					678:682	a rat model	672:682	a rat model	672:682	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	8	42	theme	lipid	1152:1156	arg1	metabolism					1158:1167	lipid metabolism	1152:1167	lipid metabolism	1152:1167	In contrast, combined BL and XOS supplementation inhibited body weight gain and returned lipid metabolism to normal.					
32509874	4	43	theme	probiotic	552:560	arg1	BL					603:604	BL	603:604	BL	603:604	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	4	43	theme	probiotic	552:560	arg1	Zhengchangsheng®					585:600	probiotic Bacillus licheniformis Zhengchangsheng®	552:600	probiotic Bacillus licheniformis Zhengchangsheng® (BL)	552:605	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	8	44	theme	BL	1085:1086	arg1	supplementation					1096:1110	combined BL and XOS supplementation	1076:1110	combined BL and XOS supplementation	1076:1110	In contrast, combined BL and XOS supplementation inhibited body weight gain and returned lipid metabolism to normal.					
32509874	5	45	theme	Dawley	754:759	arg1	rats					766:769	male Sprague Dawley (SD) rats	741:769	male Sprague Dawley (SD) rats	741:769	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	6	46	theme	inflammatory	886:897	arg1	biomarkers					899:908	inflammatory biomarkers	886:908	inflammatory biomarkers	886:908	Lipid profiles, inflammatory biomarkers, and microbiota composition were analyzed at the end of the experiment.					
32509874	5	47	theme	normal	782:787	arg1	diet					793:796	a normal fat diet	780:796	a normal fat diet (CON)	780:802	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	5	47	theme	normal	782:787	arg1	CON					799:801	CON	799:801	CON	799:801	Five groups of male Sprague Dawley (SD) rats were fed a normal fat diet (CON) or an HFD with or without BL and XOS supplementation for 3 weeks.					
32509874	11	48	theme	HFD-induced	1646:1656	arg1	obesity					1658:1664	HFD-induced obesity	1646:1664	HFD-induced obesity in rats	1646:1672	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	3	49	theme	human	428:432	arg1	health					434:439	human health	428:439	human health	428:439	However, their benefits in promoting human health and the underlying mechanisms still need to be further characterized.					
32509874	4	50	theme	licheniformis	571:583	arg1	BL					603:604	BL	603:604	BL	603:604	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	4	50	theme	licheniformis	571:583	arg1	Zhengchangsheng®					585:600	probiotic Bacillus licheniformis Zhengchangsheng®	552:600	probiotic Bacillus licheniformis Zhengchangsheng® (BL)	552:605	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	10	51	theme	microbial	1478:1486	arg1	variations					1488:1497	microbial variations	1478:1497	microbial variations	1478:1497	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	10	52	theme	LPS	1432:1434	arg1	related					1467:1473	related	1467:1473	related	1467:1473	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	10	52	theme	LPS	1432:1434	arg1	level					1436:1440	the serum LPS level	1422:1440	the serum LPS level	1422:1440	In addition, supplements of combined BL and XOS obviously reduced the serum LPS level, which was significantly related to microbial variations.					
32509874	11	53	theme	gut	1544:1546	arg1	microbiota					1548:1557	the gut microbiota	1540:1557	the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation	1540:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	9	54	theme	XOS	1200:1202	arg1	administration					1204:1217	XOS administration	1200:1217	XOS administration	1200:1217	Furthermore, BL and XOS administration changed the gut microbiota composition and modulated specific bacteria such as Prevotellaceae, Desulfovibrionaceae, and Ruminococcaceae.					
32509874	11	55	theme	microbiota	1548:1557	arg1	modulation					1526:1535	modulation	1526:1535	modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation	1526:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	11	56	theme	supplementation	1605:1619	arg1	result					1564:1569	a result	1562:1569	a result of probiotic BL and prebiotic XOS supplementation	1562:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	6	57	theme	experiment	970:979	arg1	end					959:961	the end	955:961	the end of the experiment	955:979	Lipid profiles, inflammatory biomarkers, and microbiota composition were analyzed at the end of the experiment.					
32509874	11	58	theme	probiotic	1574:1582	arg1	BL					1584:1585	probiotic BL	1574:1585	probiotic BL	1574:1585	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	4	59	theme	60 kcal	693:699	arg1	HF					689:690	HF	689:690	HF (60 kcal %) diet-induced obesity	689:723	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	4	59	theme	60 kcal	693:699	arg1	%					701:701	60 kcal %	693:701	60 kcal %	693:701	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	2	60	from	effects	312:318	arg1	obesity					352:358	obesity	352:358	obesity	352:358	The beneficial effects of probiotics and prebiotics on obesity have been reported previously.					
32509874	1	61	theme	low-grade	232:240	arg1	inflammation					242:253	low-grade inflammation	232:253	low-grade inflammation leading to diverse inflammatory diseases	232:294	Gut dysbiosis induced by high-fat diet (HFD) may result in low-grade inflammation leading to diverse inflammatory diseases.					
32509874	8	62	theme	combined	1076:1083	arg1	supplementation					1096:1110	combined BL and XOS supplementation	1076:1110	combined BL and XOS supplementation	1076:1110	In contrast, combined BL and XOS supplementation inhibited body weight gain and returned lipid metabolism to normal.					
32509874	4	63	with	health	662:667	arg1	obesity					717:723	HF (60 kcal %) diet-induced obesity	689:723	HF (60 kcal %) diet-induced obesity	689:723	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	11	64	theme	BL	1584:1585	arg1	result					1564:1569	a result	1562:1569	a result of probiotic BL and prebiotic XOS supplementation	1562:1619	Our findings suggest that modulation of the gut microbiota as a result of probiotic BL and prebiotic XOS supplementation has a positive effect on HFD-induced obesity in rats.					
32509874	4	65	theme	model	678:682	arg1	health					662:667	the health	658:667	the health of a rat model with HF (60 kcal %) diet-induced obesity	658:723	This study is aimed at understanding how probiotic Bacillus licheniformis Zhengchangsheng® (BL) and prebiotic xylooligosaccharides (XOS) influence the health of a rat model with HF (60 kcal %) diet-induced obesity.					
32509874	0	66	from	Microbiota	153:162	arg1	Rats					167:170	Rats	167:170	Rats	167:170	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	0	67	theme	Xylooligosaccharides	73:92	arg1	Supplements					5:15	Oral Supplements	0:15	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides	0:92	Oral Supplements of Combined Bacillus licheniformis Zhengchangsheng® and Xylooligosaccharides Improve High-Fat Diet-Induced Obesity and Modulate the Gut Microbiota in Rats.					
32509874	6	68	theme	Lipid	870:874	arg1	profiles					876:883	Lipid profiles	870:883	Lipid profiles	870:883	Lipid profiles, inflammatory biomarkers, and microbiota composition were analyzed at the end of the experiment.					
32509874	1	69	theme	Gut	173:175	arg1	dysbiosis					177:185	Gut dysbiosis	173:185	Gut dysbiosis induced by high-fat diet (HFD)	173:216	Gut dysbiosis induced by high-fat diet (HFD) may result in low-grade inflammation leading to diverse inflammatory diseases.					
32509874	8	70	theme	XOS	1092:1094	arg1	supplementation					1096:1110	combined BL and XOS supplementation	1076:1110	combined BL and XOS supplementation	1076:1110	In contrast, combined BL and XOS supplementation inhibited body weight gain and returned lipid metabolism to normal.					
32635954	1	0	from	reformulation	216:228	arg1	beverages					264:272	beverages	264:272	beverages	264:272	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	1	0	from	reformulation	216:228	arg1	foods					254:258	Canadian prepackaged foods	233:258	Canadian prepackaged foods	233:258	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	11	1	from	study	1628:1632	arg1	Results					1610:1616	Results	1610:1616	Results from this study	1610:1632	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	8	2	theme	%	1172:1172	arg1	reduction					1154:1162	A median sugar reduction	1139:1162	A median sugar reduction of 19·0 % (1·6 g)	1139:1180	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	3	3	from	changes	643:649	arg1	levels					660:665	sugar levels	654:665	sugar levels	654:665	The proportion of products with changes in sugar levels was determined.					
32635954	11	4	from	areas	1643:1647	arg1	supply					1661:1666	the food supply	1652:1666	the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition	1652:1804	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	4	5	theme	lower	848:852	arg1	products					839:846	products	839:846	products lower in sugar	839:861	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	7	6	theme	sugar	1045:1049	arg1	content					1051:1057	total sugar content	1039:1057	total sugar content	1039:1057	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	9	7	theme	energies	1332:1339	arg1	levels					1322:1327	median levels	1315:1327	median levels of energies and other nutrients	1315:1359	Overall, median levels of energies and other nutrients stayed the same or decreased among products reformulated to be lower in sugar, the exception was for starch, which increased.					
32635954	8	8	theme	sugar	1148:1152	arg1	reduction					1154:1162	A median sugar reduction	1139:1162	A median sugar reduction of 19·0 % (1·6 g)	1139:1180	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	6	9	theme	prepackaged	947:957	arg1	foods					959:963	foods	959:963	foods	959:963	PARTICIPANTS Canadian prepackaged foods and beverages.					
32635954	11	10	theme	sugar-focused	1734:1746	arg1	reformulation					1748:1760	sugar-focused reformulation	1734:1760	sugar-focused reformulation	1734:1760	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	0	11	theme	nutritional	123:133	arg1	composition					135:145	nutritional composition	123:145	nutritional composition of products with sugar reductions	123:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	9	12	theme	other	1345:1349	arg1	nutrients					1351:1359	other nutrients	1345:1359	other nutrients	1345:1359	Overall, median levels of energies and other nutrients stayed the same or decreased among products reformulated to be lower in sugar, the exception was for starch, which increased.					
32635954	5	13	theme	market	901:906	arg1	share					908:912	market share	901:912	market share in Canada	901:922	SETTING Largest grocery retailers by market share in Canada.					
32635954	2	14	theme	Program	567:573	arg1	products					600:607	n 6628 matched products	585:607	n 6628 matched products	585:607	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	14	theme	Program	567:573	arg1	database					575:582	Information Program database	555:582	Information Program database (n 6628 matched products)	555:608	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	7	15	theme	products	1013:1020	arg1	majority					992:999	The majority	988:999	RESULTS The majority (76·6 %) of products	980:1020	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	7	15	theme	products	1013:1020	arg1	%					1007:1007	76·6 %	1002:1007	76·6 %	1002:1007	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	11	16	theme	overall	1774:1780	arg1	composition					1794:1804	overall nutritional composition	1774:1804	overall nutritional composition	1774:1804	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	5	17	theme	Largest	872:878	arg1	retailers					888:896	Largest grocery retailers	872:896	Largest grocery retailers	872:896	SETTING Largest grocery retailers by market share in Canada.					
32635954	4	18	theme	signed-rank	692:702	arg1	test					704:707	Wilcoxon signed-rank test	683:707	Wilcoxon signed-rank test	683:707	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	3	19	with	proportion	615:624	arg1	changes					643:649	changes	643:649	changes in sugar levels	643:665	The proportion of products with changes in sugar levels was determined.					
32635954	4	20	used	used	713:716	arg2	test					704:707	Wilcoxon signed-rank test	683:707	Wilcoxon signed-rank test	683:707	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	0	21	from	Reformulation	0:12	arg1	changes					112:118	resultant changes	102:118	resultant changes in nutritional composition of products with sugar reductions	102:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	0	21	from	Reformulation	0:12	arg1	beverages					66:74	beverages	66:74	beverages	66:74	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	0	21	from	Reformulation	0:12	arg1	foods					56:60	Canadian prepackaged foods	35:60	Canadian prepackaged foods	35:60	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	4	22	theme	overall	753:759	arg1	levels					746:751	sugar levels	740:751	sugar levels overall for products lower or higher in sugar	740:797	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	11	23	theme	nutritional	1782:1792	arg1	composition					1794:1804	overall nutritional composition	1774:1804	overall nutritional composition	1774:1804	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	8	24	theme	18·0	1250:1253	arg1	%					1255:1255	%	1255:1255	%	1255:1255	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	11	25	theme	reformulation	1748:1760	arg1	consequences					1718:1729	unintended consequences	1707:1729	unintended consequences of sugar-focused reformulation	1707:1760	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	5	26	from	share	908:912	arg1	Canada					917:922	Canada	917:922	Canada	917:922	SETTING Largest grocery retailers by market share in Canada.					
32635954	7	27	contain	had	1022:1024	arg1	RESULTS					980:986	RESULTS	980:986	RESULTS The majority (76·6 %) of products	980:1020	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	7	27	contain	had	1022:1024	arg2	change					1029:1034	no change	1026:1034	no change	1026:1034	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	10	28	theme	Limited	1499:1505	arg1	progress					1507:1514	CONCLUSIONS Limited progress	1487:1514	CONCLUSIONS Limited progress	1487:1514	CONCLUSIONS Limited progress was made to reformulate foods and beverages to be lower in total sugar between 2013 and 2017.					
32635954	4	29	from	changes	729:735	arg1	composition					823:833	nutrient composition	814:833	nutrient composition for products lower in sugar	814:861	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	4	29	from	changes	729:735	arg1	levels					746:751	sugar levels	740:751	sugar levels overall for products lower or higher in sugar	740:797	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	4	30	theme	sugar	740:744	arg1	levels					746:751	sugar levels	740:751	sugar levels overall for products lower or higher in sugar	740:797	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	7	31	dep	RESULTS	980:986	arg1	majority					992:999	The majority	988:999	RESULTS The majority (76·6 %) of products	980:1020	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	7	31	dep	RESULTS	980:986	arg1	%					1007:1007	76·6 %	1002:1007	76·6 %	1002:1007	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	2	32	theme	Longitudinal	423:434	arg1	examination					436:446	DESIGN Longitudinal examination	416:446	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food	416:547	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	0	33	theme	sugar	17:21	arg1	contents					23:30	sugar contents	17:30	sugar contents	17:30	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	1	34	dep	OBJECTIVE	182:190	arg1	identify					300:307	identify	300:307	identify changes in the nutritional composition of the foods and beverages	300:373	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	1	34	dep	OBJECTIVE	182:190	arg1	quantify					195:202	quantify	195:202	quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017	195:294	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	2	35	theme	present	471:477	arg1	foods					451:455	foods	451:455	foods	451:455	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	3	36	theme	products	629:636	arg1	proportion					615:624	The proportion	611:624	The proportion of products with changes in sugar levels	611:665	The proportion of products with changes in sugar levels was determined.					
32635954	0	37	theme	prepackaged	44:54	arg1	foods					56:60	Canadian prepackaged foods	35:60	Canadian prepackaged foods	35:60	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	3	38	theme	sugar	654:658	arg1	levels					660:665	sugar levels	654:665	sugar levels	654:665	The proportion of products with changes in sugar levels was determined.					
32635954	11	39	theme	unintended	1707:1716	arg1	consequences					1718:1729	unintended consequences	1707:1729	unintended consequences of sugar-focused reformulation	1707:1760	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	1	40	theme	prepackaged	242:252	arg1	foods					254:258	Canadian prepackaged foods	233:258	Canadian prepackaged foods	233:258	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	0	41	from	changes	112:118	arg1	composition					135:145	nutritional composition	123:145	nutritional composition of products with sugar reductions	123:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	2	42	theme	matched	592:598	arg1	products					600:607	n 6628 matched products	585:607	n 6628 matched products	585:607	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	42	theme	matched	592:598	arg1	database					575:582	Information Program database	555:582	Information Program database (n 6628 matched products)	555:608	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	1	43	theme	total	204:208	arg1	reformulation					216:228	total sugar reformulation	204:228	total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017	204:294	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	1	44	from	changes	309:315	arg1	composition					336:346	the nutritional composition	320:346	the nutritional composition of the foods and beverages	320:373	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	2	45	theme	n	585:585	arg1	products					600:607	n 6628 matched products	585:607	n 6628 matched products	585:607	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	45	theme	n	585:585	arg1	database					575:582	Information Program database	555:582	Information Program database (n 6628 matched products)	555:608	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	4	46	theme	nutrient	814:821	arg1	composition					823:833	nutrient composition	814:833	nutrient composition for products lower in sugar	814:861	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	8	47	theme	higher	1289:1294	arg1	products					1280:1287	products	1280:1287	products higher in sugar	1280:1303	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	9	48	theme	median	1315:1320	arg1	levels					1322:1327	median levels	1315:1327	median levels of energies and other nutrients	1315:1359	Overall, median levels of energies and other nutrients stayed the same or decreased among products reformulated to be lower in sugar, the exception was for starch, which increased.					
32635954	7	49	theme	total	1039:1043	arg1	content					1051:1057	total sugar content	1039:1057	total sugar content	1039:1057	RESULTS The majority (76·6 %) of products had no change in total sugar content, 12·4 % were reformulated to be lower in sugar and 11·0 % were higher in sugar.					
32635954	8	50	dep	%	1255:1255	arg1	g					1262:1262	1·5 g	1258:1262	1·5 g	1258:1262	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	1	51	theme	sugar	210:214	arg1	reformulation					216:228	total sugar reformulation	204:228	total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017	204:294	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	8	52	theme	median	1141:1146	arg1	reduction					1154:1162	A median sugar reduction	1139:1162	A median sugar reduction of 19·0 % (1·6 g)	1139:1180	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	0	53	from	beverages	66:74	arg1	composition					135:145	nutritional composition	123:145	nutritional composition of products with sugar reductions	123:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	0	54	theme	products	150:157	arg1	composition					135:145	nutritional composition	123:145	nutritional composition of products with sugar reductions	123:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	1	55	theme	total	403:407	arg1	sugar					409:413	total sugar	403:413	total sugar	403:413	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	11	56	theme	composition	1794:1804	arg1	terms					1765:1769	terms	1765:1769	terms of overall nutritional composition	1765:1804	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	4	57	from	changes	803:809	arg1	composition					823:833	nutrient composition	814:833	nutrient composition for products lower in sugar	814:861	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	4	57	from	changes	803:809	arg1	levels					746:751	sugar levels	740:751	sugar levels overall for products lower or higher in sugar	740:797	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	9	58	theme	nutrients	1351:1359	arg1	levels					1322:1327	median levels	1315:1327	median levels of energies and other nutrients	1315:1359	Overall, median levels of energies and other nutrients stayed the same or decreased among products reformulated to be lower in sugar, the exception was for starch, which increased.					
32635954	11	59	theme	food	1656:1659	arg1	supply					1661:1666	the food supply	1652:1666	the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition	1652:1804	Results from this study identify areas in the food supply where attention may be needed to avoid unintended consequences of sugar-focused reformulation in terms of overall nutritional composition.					
32635954	0	60	theme	sugar	164:168	arg1	reductions					170:179	sugar reductions	164:179	sugar reductions	164:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	2	61	theme	Information	555:565	arg1	products					600:607	n 6628 matched products	585:607	n 6628 matched products	585:607	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	61	theme	Information	555:565	arg1	database					575:582	Information Program database	555:582	Information Program database (n 6628 matched products)	555:608	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	8	62	theme	lower	1206:1210	arg1	products					1197:1204	products	1197:1204	products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar	1197:1303	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	2	63	theme	2017	496:499	arg1	collections					501:511	both 2013 and 2017 collections	482:511	collections	501:511	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	5	64	theme	grocery	880:886	arg1	retailers					888:896	Largest grocery retailers	872:896	Largest grocery retailers	872:896	SETTING Largest grocery retailers by market share in Canada.					
32635954	0	65	from	foods	56:60	arg1	composition					135:145	nutritional composition	123:145	nutritional composition of products with sugar reductions	123:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	2	66	theme	2013	487:490	arg1	collections					501:511	both 2013 and 2017 collections	482:511	collections	501:511	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	4	67	theme	Wilcoxon	683:690	arg1	test					704:707	Wilcoxon signed-rank test	683:707	Wilcoxon signed-rank test	683:707	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	0	68	with	products	150:157	arg1	reductions					170:179	sugar reductions	164:179	sugar reductions	164:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	1	69	theme	foods	355:359	arg1	composition					336:346	the nutritional composition	320:346	the nutritional composition of the foods and beverages	320:373	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	2	70	theme	University	520:529	arg1	collections					501:511	both 2013 and 2017 collections	482:511	collections	501:511	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	71	from	present	471:477	arg1	collections					501:511	both 2013 and 2017 collections	482:511	collections	501:511	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	0	72	theme	resultant	102:110	arg1	changes					112:118	resultant changes	102:118	resultant changes in nutritional composition of products with sugar reductions	102:179	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	1	73	theme	beverages	365:373	arg1	composition					336:346	the nutritional composition	320:346	the nutritional composition of the foods and beverages	320:373	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	2	74	attach	present	471:477	arg1	collections					501:511	both 2013 and 2017 collections	482:511	collections	501:511	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	74	attach	present	471:477	arg2	foods					451:455	foods	451:455	foods	451:455	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	8	75	theme	median	1243:1248	arg1	increase					1265:1272	a median 18·0 % (1·5 g) increase	1241:1272	a median 18·0 % (1·5 g) increase among products higher in sugar	1241:1303	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	2	76	theme	foods	451:455	arg1	examination					436:446	DESIGN Longitudinal examination	416:446	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food	416:547	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	8	77	theme	%	1255:1255	arg1	increase					1265:1272	a median 18·0 % (1·5 g) increase	1241:1272	a median 18·0 % (1·5 g) increase among products higher in sugar	1241:1303	A median sugar reduction of 19·0 % (1·6 g) was seen among products lower in sugar which was offset by a median 18·0 % (1·5 g) increase among products higher in sugar.					
32635954	10	78	theme	CONCLUSIONS	1487:1497	arg1	progress					1507:1514	CONCLUSIONS Limited progress	1487:1514	CONCLUSIONS Limited progress	1487:1514	CONCLUSIONS Limited progress was made to reformulate foods and beverages to be lower in total sugar between 2013 and 2017.					
32635954	0	79	theme	contents	23:30	arg1	Reformulation					0:12	Reformulation	0:12	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.	0:180	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	2	80	theme	DESIGN	416:421	arg1	examination					436:446	DESIGN Longitudinal examination	416:446	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food	416:547	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	0	81	theme	Canadian	35:42	arg1	foods					56:60	Canadian prepackaged foods	35:60	Canadian prepackaged foods	35:60	Reformulation of sugar contents in Canadian prepackaged foods and beverages between 2013 and 2017 and resultant changes in nutritional composition of products with sugar reductions.					
32635954	2	82	theme	beverages	461:469	arg1	examination					436:446	DESIGN Longitudinal examination	416:446	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food	416:547	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	2	83	from	collections	501:511	arg1	present					471:477	present	471:477	present	471:477	DESIGN Longitudinal examination of foods and beverages present in both 2013 and 2017 collections of the University of Toronto's Food Label Information Program database (n 6628 matched products).					
32635954	1	84	theme	nutritional	324:334	arg1	composition					336:346	the nutritional composition	320:346	the nutritional composition of the foods and beverages	320:373	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	4	85	theme	lower	774:778	arg1	products					765:772	products	765:772	products lower or higher in sugar	765:797	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	1	86	theme	Canadian	233:240	arg1	foods					254:258	Canadian prepackaged foods	233:258	Canadian prepackaged foods	233:258	OBJECTIVE To quantify total sugar reformulation in Canadian prepackaged foods and beverages between 2013 and 2017 and identify changes in the nutritional composition of the foods and beverages reformulated to be lower in total sugar.					
32635954	4	87	theme	higher	783:788	arg1	products					765:772	products	765:772	products lower or higher in sugar	765:797	Wilcoxon signed-rank test was used to examine changes in sugar levels overall for products lower or higher in sugar and changes in nutrient composition for products lower in sugar.					
32635954	10	88	theme	total	1575:1579	arg1	sugar					1581:1585	total sugar	1575:1585	total sugar between 2013 and 2017	1575:1607	CONCLUSIONS Limited progress was made to reformulate foods and beverages to be lower in total sugar between 2013 and 2017.					
33376239	5	0	theme	of Daphnia	807:816	arg1	magna					818:822	the exoskeleton of Daphnia magna	791:822	the exoskeleton of Daphnia magna	791:822	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	4	1	theme	conspicuous	556:566	arg1	helmets					610:616	helmets	610:616	helmets	610:616	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	4	1	theme	conspicuous	556:566	arg1	defenses					592:599	conspicuous inducible morphological defenses	556:599	conspicuous inducible morphological defenses	556:599	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	1	2	from	niche	200:204	arg1	Earth					209:213	Earth	209:213	Earth	209:213	The widespread distribution of Crustacea across every aquatic ecological niche on Earth is enabled due to their exoskeleton's versatile properties.					
33376239	7	3	from	defenses	1245:1252	arg1	Daphnia					1257:1263	Daphnia	1257:1263	Daphnia	1257:1263	We, therefore, highlight the importance of calcium-biominerals for inducible morphological defenses in Daphnia.					
33376239	0	4	theme	Daphnia	83:89	arg1	magna					91:95	the crustacean Daphnia magna	68:95	the crustacean Daphnia magna	68:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	1	5	theme	aquatic	181:187	arg1	niche					200:204	every aquatic ecological niche	175:204	every aquatic ecological niche on Earth	175:213	The widespread distribution of Crustacea across every aquatic ecological niche on Earth is enabled due to their exoskeleton's versatile properties.					
33376239	0	6	theme	micro-Raman	101:111	arg1	spectroscopy					113:124	micro-Raman spectroscopy	101:124	micro-Raman spectroscopy	101:124	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	4	7	theme	morphological	578:590	arg1	helmets					610:616	helmets	610:616	helmets	610:616	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	4	7	theme	morphological	578:590	arg1	defenses					592:599	conspicuous inducible morphological defenses	556:599	conspicuous inducible morphological defenses	556:599	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	5	8	theme	chemical	767:774	arg1	composition					776:786	the chemical composition	763:786	the chemical composition of the exoskeleton of Daphnia magna	763:822	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	4	9	theme	zooplankton	530:540	arg1	Daphnia					542:548	the freshwater zooplankton Daphnia	515:548	the freshwater zooplankton Daphnia	515:548	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	2	10	theme	exoskeleton	308:318	arg1	mineralization					286:299	mineralization	286:299	Especially mineralization of the exoskeleton	275:318	Especially mineralization of the exoskeleton provides protection against diverse environmental threats.					
33376239	5	11	theme	embedded	896:903	arg1	ACC					934:936	ACC	934:936	ACC	934:936	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	5	11	theme	embedded	896:903	arg1	carbonate					923:931	embedded amorphous calcium carbonate	896:931	embedded amorphous calcium carbonate (ACC)	896:937	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	4	12	theme	freshwater	519:528	arg1	Daphnia					542:548	the freshwater zooplankton Daphnia	515:548	the freshwater zooplankton Daphnia	515:548	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	6	13	from	mechanisms	1032:1041	arg1	form					1050:1053	the form	1046:1053	the form	1046:1053	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	6	14	theme	enhanced	1125:1132	arg1	stability					1143:1151	enhanced carapace stability	1125:1151	enhanced carapace stability	1125:1151	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	1	15	theme	ecological	189:198	arg1	niche					200:204	every aquatic ecological niche	175:204	every aquatic ecological niche on Earth	175:213	The widespread distribution of Crustacea across every aquatic ecological niche on Earth is enabled due to their exoskeleton's versatile properties.					
33376239	6	16	theme	defense	1024:1030	arg1	mechanisms					1032:1041	inducible defense mechanisms	1014:1041	inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability	1014:1151	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	5	17	theme	amorphous	905:913	arg1	ACC					934:936	ACC	934:936	ACC	934:936	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	5	17	theme	amorphous	905:913	arg1	carbonate					923:931	embedded amorphous calcium carbonate	896:931	embedded amorphous calcium carbonate (ACC)	896:937	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	7	18	theme	morphological	1231:1243	arg1	defenses					1245:1252	inducible morphological defenses	1221:1252	inducible morphological defenses in Daphnia	1221:1263	We, therefore, highlight the importance of calcium-biominerals for inducible morphological defenses in Daphnia.					
33376239	5	19	theme	magna	818:822	arg1	composition					776:786	the chemical composition	763:786	the chemical composition of the exoskeleton of Daphnia magna	763:822	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	2	20	theme	environmental	356:368	arg1	threats					370:376	diverse environmental threats	348:376	diverse environmental threats	348:376	Especially mineralization of the exoskeleton provides protection against diverse environmental threats.					
33376239	0	21	from	defences	56:63	arg1	magna					91:95	the crustacean Daphnia magna	68:95	the crustacean Daphnia magna	68:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	5	22	theme	calcium	915:921	arg1	ACC					934:936	ACC	934:936	ACC	934:936	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	5	22	theme	calcium	915:921	arg1	carbonate					923:931	embedded amorphous calcium carbonate	896:931	embedded amorphous calcium carbonate (ACC)	896:937	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	5	23	with	of α-chitin	866:876	arg1	ACC					934:936	ACC	934:936	ACC	934:936	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	5	23	with	of α-chitin	866:876	arg1	carbonate					923:931	embedded amorphous calcium carbonate	896:931	embedded amorphous calcium carbonate (ACC)	896:937	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	6	24	theme	inducible	1014:1022	arg1	mechanisms					1032:1041	inducible defense mechanisms	1014:1041	inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability	1014:1151	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	2	25	theme	diverse	348:354	arg1	threats					370:376	diverse environmental threats	348:376	diverse environmental threats	348:376	Especially mineralization of the exoskeleton provides protection against diverse environmental threats.					
33376239	3	26	theme	entomostracans	412:425	arg1	exoskeleton					392:402	the exoskeleton	388:402	the exoskeleton of some entomostracans	388:425	Thereby, the exoskeleton of some entomostracans is extremely phenotypically plastic, especially in response to predators.					
33376239	3	26	theme	entomostracans	412:425	arg1	plastic					455:461	plastic	455:461	plastic	455:461	Thereby, the exoskeleton of some entomostracans is extremely phenotypically plastic, especially in response to predators.					
33376239	5	27	with	proteins	882:889	arg1	ACC					934:936	ACC	934:936	ACC	934:936	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	5	27	with	proteins	882:889	arg1	carbonate					923:931	embedded amorphous calcium carbonate	896:931	embedded amorphous calcium carbonate (ACC)	896:937	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	0	28	theme	chemistry	15:23	arg1	defences					56:63	the chemistry behind inducible morphological defences	11:63	the chemistry behind inducible morphological defences in the crustacean Daphnia magna	11:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	6	29	theme	carapace	1134:1141	arg1	stability					1143:1151	enhanced carapace stability	1125:1151	enhanced carapace stability	1125:1151	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	0	30	theme	morphological	42:54	arg1	defences					56:63	the chemistry behind inducible morphological defences	11:63	the chemistry behind inducible morphological defences in the crustacean Daphnia magna	11:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	5	31	theme	first	752:756	arg1	time					758:761	the first time	748:761	the first time	748:761	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	0	32	theme	inducible	32:40	arg1	defences					56:63	the chemistry behind inducible morphological defences	11:63	the chemistry behind inducible morphological defences in the crustacean Daphnia magna	11:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	6	33	theme	chemical	981:988	arg1	changes					990:996	the exoskeleton's chemical changes	963:996	the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability	963:1151	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	7	34	theme	calcium-biominerals	1197:1215	arg1	importance					1183:1192	the importance	1179:1192	the importance of calcium-biominerals for inducible morphological defenses in Daphnia	1179:1263	We, therefore, highlight the importance of calcium-biominerals for inducible morphological defenses in Daphnia.					
33376239	0	35	theme	behind	25:30	arg1	defences					56:63	the chemistry behind inducible morphological defences	11:63	the chemistry behind inducible morphological defences in the crustacean Daphnia magna	11:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	6	36	theme	mineralization	1075:1088	arg1	form					1050:1053	the form	1046:1053	the form	1046:1053	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	5	37	theme	Raman	831:835	arg1	spectroscopy					837:848	Raman spectroscopy	831:848	Raman spectroscopy	831:848	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	4	38	theme	inducible	568:576	arg1	helmets					610:616	helmets	610:616	helmets	610:616	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	4	38	theme	inducible	568:576	arg1	defenses					592:599	conspicuous inducible morphological defenses	556:599	conspicuous inducible morphological defenses	556:599	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	1	39	theme	widespread	131:140	arg1	distribution					142:153	The widespread distribution	127:153	The widespread distribution of Crustacea across every aquatic ecological niche on Earth	127:213	The widespread distribution of Crustacea across every aquatic ecological niche on Earth is enabled due to their exoskeleton's versatile properties.					
33376239	6	40	theme	substantial	1063:1073	arg1	mineralization					1075:1088	more substantial mineralization	1058:1088	more substantial mineralization	1058:1088	Furthermore, we reveal the exoskeleton's chemical changes associated with inducible defense mechanisms in the form of more substantial mineralization, which is probably correlated with enhanced carapace stability.					
33376239	3	41	from	response	478:485	arg1	exoskeleton					392:402	the exoskeleton	388:402	the exoskeleton of some entomostracans	388:425	Thereby, the exoskeleton of some entomostracans is extremely phenotypically plastic, especially in response to predators.					
33376239	3	41	from	response	478:485	arg1	plastic					455:461	plastic	455:461	plastic	455:461	Thereby, the exoskeleton of some entomostracans is extremely phenotypically plastic, especially in response to predators.					
33376239	4	42	theme	exoskeleton	657:667	arg1	stability					640:648	the stability	636:648	the stability of its exoskeleton, which renders them less vulnerable to predation	636:716	For instance, the freshwater zooplankton Daphnia forms conspicuous inducible morphological defenses, such as helmets, and can increase the stability of its exoskeleton, which renders them less vulnerable to predation.					
33376239	0	43	theme	crustacean	72:81	arg1	magna					91:95	the crustacean Daphnia magna	68:95	the crustacean Daphnia magna	68:95	Uncovering the chemistry behind inducible morphological defences in the crustacean Daphnia magna via micro-Raman spectroscopy.					
33376239	1	44	theme	Crustacea	158:166	arg1	distribution					142:153	The widespread distribution	127:153	The widespread distribution of Crustacea across every aquatic ecological niche on Earth	127:213	The widespread distribution of Crustacea across every aquatic ecological niche on Earth is enabled due to their exoskeleton's versatile properties.					
33376239	5	45	theme	exoskeleton	795:805	arg1	magna					818:822	the exoskeleton of Daphnia magna	791:822	the exoskeleton of Daphnia magna	791:822	In this study, we reveal for the first time the chemical composition of the exoskeleton of Daphnia magna, using Raman spectroscopy, to be composed of α-chitin and proteins with embedded amorphous calcium carbonate (ACC).					
33376239	3	46	from	plastic	455:461	arg1	response					478:485	response	478:485	response to predators	478:498	Thereby, the exoskeleton of some entomostracans is extremely phenotypically plastic, especially in response to predators.					
33376239	7	47	theme	inducible	1221:1229	arg1	defenses					1245:1252	inducible morphological defenses	1221:1252	inducible morphological defenses in Daphnia	1221:1263	We, therefore, highlight the importance of calcium-biominerals for inducible morphological defenses in Daphnia.					
33376239	1	48	theme	versatile	253:261	arg1	properties					263:272	their exoskeleton's versatile properties	233:272	their exoskeleton's versatile properties	233:272	The widespread distribution of Crustacea across every aquatic ecological niche on Earth is enabled due to their exoskeleton's versatile properties.					
33094998	2	0	theme	pharmacological	456:470	arg1	strategies					472:481	conventional pharmacological strategies	443:481	conventional pharmacological strategies	443:481	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	3	1	theme	Recent	484:489	arg1	molecules					569:577	small molecules	563:577	small molecules that bind carbohydrates	563:601	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	3	1	theme	Recent	484:489	arg1	advances					491:498	Recent advances	484:498	Recent advances in the development of synthetic carbohydrate receptors (SCRs)	484:560	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	2	2	theme	conventional	443:454	arg1	strategies					472:481	conventional pharmacological strategies	443:481	conventional pharmacological strategies	443:481	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	3	3	theme	targets	683:689	arg1	purview					662:668	the purview	658:668	the purview of druggable targets	658:689	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	5	4	theme	design	894:899	arg1	strategies					901:910	Common SCR design strategies	883:910	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions	883:990	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	3	5	theme	carbohydrate-receptor	616:636	arg1	interactions					638:649	carbohydrate-receptor interactions	616:649	carbohydrate-receptor interactions	616:649	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	3	6	from	advances	491:498	arg1	development					507:517	the development	503:517	the development of synthetic carbohydrate receptors (SCRs)	503:560	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	3	7	theme	synthetic	522:530	arg1	SCRs					556:559	SCRs	556:559	SCRs	556:559	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	3	7	theme	synthetic	522:530	arg1	receptors					545:553	synthetic carbohydrate receptors	522:553	synthetic carbohydrate receptors (SCRs)	522:560	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	4	8	theme	interactions	742:753	arg1	role					712:715	the role	708:715	the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro	708:880	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	3	9	theme	carbohydrate	532:543	arg1	SCRs					556:559	SCRs	556:559	SCRs	556:559	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	3	9	theme	carbohydrate	532:543	arg1	receptors					545:553	synthetic carbohydrate receptors	522:553	synthetic carbohydrate receptors (SCRs)	522:560	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	5	10	theme	ester	929:933	arg1	formation					935:943	boronic ester formation	921:943	boronic ester formation	921:943	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	6	11	theme	nonglucosidic	1173:1185	arg1	specificity					1187:1197	nonglucosidic specificity	1173:1197	nonglucosidic specificity	1173:1197	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	6	12	theme	natural	1059:1065	arg1	flexibility					1101:1111	natural glycan-binding proteins-including flexibility	1059:1111	natural glycan-binding proteins-including flexibility	1059:1111	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	1	13	theme	Carbohydrate-receptor	77:97	arg1	interactions					99:110	Carbohydrate-receptor interactions	77:110	Carbohydrate-receptor interactions	77:110	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	6	14	theme	cooperativity	1114:1126	arg1	idiosyncrasies					1041:1054	the idiosyncrasies	1037:1054	the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design	1037:1160	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	7	15	theme	SCR	1229:1231	arg1	design					1233:1238	SCR design	1229:1238	SCR design	1229:1238	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	0	16	theme	Synthetic	9:17	arg1	Receptors					32:40	Flexible Synthetic Carbohydrate Receptors	0:40	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.	0:75	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.					
33094998	4	17	theme	viral	856:860	arg1	infections					862:871	viral infections	856:871	viral infections	856:871	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	2	18	theme	conserved	299:307	arg1	structures					309:318	the conserved structures	295:318	the conserved structures of the glycans involved in docking	295:353	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	6	19	theme	flexibility	1101:1111	arg1	idiosyncrasies					1041:1054	the idiosyncrasies	1037:1054	the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design	1037:1160	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	0	20	theme	Flexible	0:7	arg1	Receptors					32:40	Flexible Synthetic Carbohydrate Receptors	0:40	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.	0:75	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.					
33094998	4	21	theme	carbohydrate-receptor	720:740	arg1	interactions					742:753	carbohydrate-receptor interactions	720:753	carbohydrate-receptor interactions	720:753	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	6	22	theme	proteins-including	1082:1099	arg1	flexibility					1101:1111	natural glycan-binding proteins-including flexibility	1059:1111	natural glycan-binding proteins-including flexibility	1059:1111	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	7	23	theme	pressing	1435:1442	arg1	HIV					1480:1482	HIV	1480:1482	HIV	1480:1482	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	23	theme	pressing	1435:1442	arg1	Dengue					1485:1490	Dengue	1485:1490	Dengue	1485:1490	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	23	theme	pressing	1435:1442	arg1	SARS-CoV-2					1514:1523	SARS-CoV-2	1514:1523	SARS-CoV-2	1514:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	23	theme	pressing	1435:1442	arg1	diseases					1460:1467	the most pressing and untreatable diseases	1426:1467	the most pressing and untreatable diseases	1426:1467	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	23	theme	pressing	1435:1442	arg1	Zika					1493:1496	Zika	1493:1496	Zika	1493:1496	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	23	theme	pressing	1435:1442	arg1	influenza					1499:1507	influenza	1499:1507	influenza	1499:1507	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	3	24	theme	receptors	545:553	arg1	development					507:517	the development	503:517	the development of synthetic carbohydrate receptors (SCRs)	503:560	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	6	25	theme	glycan-binding	1067:1080	arg1	flexibility					1101:1111	natural glycan-binding proteins-including flexibility	1059:1111	natural glycan-binding proteins-including flexibility	1059:1111	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	0	26	theme	Carbohydrate	19:30	arg1	Receptors					32:40	Flexible Synthetic Carbohydrate Receptors	0:40	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.	0:75	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.					
33094998	5	27	theme	noncovalent	967:977	arg1	interactions					979:990	noncovalent interactions	967:990	noncovalent interactions	967:990	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	1	28	theme	necessary	194:202	arg1	docking					181:187	this docking	176:187	this docking	176:187	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	1	28	theme	necessary	194:202	arg1	step					204:207	a necessary step	192:207	a necessary step in the virus life cycle that precedes infection and, ultimately, replication	192:284	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	2	29	theme	undruggable	383:393	arg1	meaning					397:403	meaning	397:403	meaning	397:403	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	2	29	theme	undruggable	383:393	arg1	"					394:394	"undruggable"	382:394	"undruggable"	382:394	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	4	30	theme	recent	802:807	arg1	results					809:815	recent results	802:815	recent results demonstrating their ability to prevent viral infections in vitro	802:880	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	1	31	from	step	204:207	arg1	cycle					227:231	the virus life cycle	212:231	the virus life cycle	212:231	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	0	32	theme	Viral	59:63	arg1	Attachment					65:74	Viral Attachment	59:74	Viral Attachment	59:74	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.					
33094998	5	33	theme	Common	883:888	arg1	strategies					901:910	Common SCR design strategies	883:910	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions	883:990	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	0	34	theme	Attachment	65:74	arg1	Inhibitors					45:54	Inhibitors	45:54	Inhibitors of Viral Attachment	45:74	Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.					
33094998	6	35	theme	SCR	1151:1153	arg1	design					1155:1160	multivalency-into SCR design	1133:1160	multivalency-into SCR design	1133:1160	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	2	36	theme	glycans	327:333	arg1	structures					309:318	the conserved structures	295:318	the conserved structures of the glycans involved in docking	295:353	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	5	37	theme	metal	946:950	arg1	chelation					952:960	metal chelation	946:960	metal chelation	946:960	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	7	38	gly	glycosylated	1375:1386	arg1	cause					1409:1413	the cause	1405:1413	the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1405:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	38	gly	glycosylated	1375:1386	arg1	viruses					1348:1354	enveloped viruses	1338:1354	enveloped viruses	1338:1354	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	38	gly	glycosylated	1375:1386	arg1	viroids					1388:1394	heavily glycosylated viroids	1367:1394	heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1367:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	3	39	theme	small	563:567	arg1	molecules					569:577	small molecules	563:577	small molecules that bind carbohydrates	563:601	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	3	39	theme	small	563:567	arg1	advances					491:498	Recent advances	484:498	Recent advances in the development of synthetic carbohydrate receptors (SCRs)	484:560	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	7	40	theme	human	1316:1320	arg1	health					1322:1327	human health	1316:1327	human health	1316:1327	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	41	theme	grave	1300:1304	arg1	threat					1306:1311	the grave threat	1296:1311	the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1296:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	3	42	theme	druggable	673:681	arg1	targets					683:689	druggable targets	673:689	druggable targets	673:689	Recent advances in the development of synthetic carbohydrate receptors (SCRs), small molecules that bind carbohydrates, could bring carbohydrate-receptor interactions within the purview of druggable targets.					
33094998	4	43	theme	SCRs	792:795	arg1	results					809:815	recent results	802:815	recent results demonstrating their ability to prevent viral infections in vitro	802:880	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	4	43	theme	SCRs	792:795	arg1	infection					764:772	viral infection	758:772	viral infection	758:772	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	4	43	theme	SCRs	792:795	arg1	evolution					779:787	the evolution	775:787	the evolution of SCRs	775:795	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	7	44	theme	some	1418:1421	arg1	cause					1409:1413	the cause	1405:1413	the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1405:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	44	theme	some	1418:1421	arg1	viroids					1388:1394	heavily glycosylated viroids	1367:1394	heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1367:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	5	45	theme	boronic	921:927	arg1	formation					935:943	boronic ester formation	921:943	boronic ester formation	921:943	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	4	46	theme	viral	758:762	arg1	infection					764:772	viral infection	758:772	viral infection	758:772	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	7	47	theme	untreatable	1448:1458	arg1	HIV					1480:1482	HIV	1480:1482	HIV	1480:1482	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	47	theme	untreatable	1448:1458	arg1	Dengue					1485:1490	Dengue	1485:1490	Dengue	1485:1490	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	47	theme	untreatable	1448:1458	arg1	SARS-CoV-2					1514:1523	SARS-CoV-2	1514:1523	SARS-CoV-2	1514:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	47	theme	untreatable	1448:1458	arg1	diseases					1460:1467	the most pressing and untreatable diseases	1426:1467	the most pressing and untreatable diseases	1426:1467	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	47	theme	untreatable	1448:1458	arg1	Zika					1493:1496	Zika	1493:1496	Zika	1493:1496	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	47	theme	untreatable	1448:1458	arg1	influenza					1499:1507	influenza	1499:1507	influenza	1499:1507	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	1	48	theme	viruses	149:155	arg1	docking					138:144	the docking	134:144	the docking of viruses to host cells	134:169	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	1	49	theme	virus	216:220	arg1	cycle					227:231	the virus life cycle	212:231	the virus life cycle	212:231	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	7	50	theme	new	1266:1268	arg1	strategies					1270:1279	new strategies	1266:1279	new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1266:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	6	51	theme	multivalency-into	1133:1149	arg1	design					1155:1160	multivalency-into SCR design	1133:1160	multivalency-into SCR design	1133:1160	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	7	52	theme	glycosylated	1375:1386	arg1	cause					1409:1413	the cause	1405:1413	the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1405:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	52	theme	glycosylated	1375:1386	arg1	viruses					1348:1354	enveloped viruses	1338:1354	enveloped viruses	1338:1354	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	52	theme	glycosylated	1375:1386	arg1	viroids					1388:1394	heavily glycosylated viroids	1367:1394	heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1367:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	53	theme	enveloped	1338:1346	arg1	viruses					1348:1354	enveloped viruses	1338:1354	enveloped viruses	1338:1354	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	7	53	theme	enveloped	1338:1346	arg1	viroids					1388:1394	heavily glycosylated viroids	1367:1394	heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2	1367:1523	These studies into SCR design and binding could lead to new strategies for mitigating the grave threat to human health posed by enveloped viruses, which are heavily glycosylated viroids that are the cause of some of the most pressing and untreatable diseases, including HIV, Dengue, Zika, influenza, and SARS-CoV-2.					
33094998	5	54	theme	SCR	890:892	arg1	strategies					901:910	Common SCR design strategies	883:910	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions	883:990	Common SCR design strategies based on boronic ester formation, metal chelation, and noncovalent interactions are discussed.					
33094998	4	55	from	role	712:715	arg1	results					809:815	recent results	802:815	recent results demonstrating their ability to prevent viral infections in vitro	802:880	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	4	55	from	role	712:715	arg1	infection					764:772	viral infection	758:772	viral infection	758:772	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	4	55	from	role	712:715	arg1	evolution					779:787	the evolution	775:787	the evolution of SCRs	775:795	Here we discuss the role of carbohydrate-receptor interactions in viral infection, the evolution of SCRs, and recent results demonstrating their ability to prevent viral infections in vitro.					
33094998	1	56	theme	life	222:225	arg1	cycle					227:231	the virus life cycle	212:231	the virus life cycle	212:231	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
33094998	2	57	theme	strategies	472:481	arg1	scope					434:438	the scope	430:438	the scope of conventional pharmacological strategies	430:481	Despite the conserved structures of the glycans involved in docking, they are still considered "undruggable", meaning these glycans are beyond the scope of conventional pharmacological strategies.					
33094998	6	58	theme	design	1155:1160	arg1	idiosyncrasies					1041:1054	the idiosyncrasies	1037:1054	the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design	1037:1160	The benefits of incorporating the idiosyncrasies of natural glycan-binding proteins-including flexibility, cooperativity, and multivalency-into SCR design to achieve nonglucosidic specificity are shown.					
33094998	1	59	theme	host	160:163	arg1	cells					165:169	host cells	160:169	host cells	160:169	Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication.					
34489047	6	0	attach	derived	918:924	arg2	pectin					911:916	pectin	911:916	pectin derived from soybean and pea	911:945	When the substrate specificity was examined, AoPME1 showed high activity towards pectin derived from soybean and pea.					
34489047	6	0	attach	derived	918:924	arg1	soybean					931:937	soybean	931:937	soybean	931:937	When the substrate specificity was examined, AoPME1 showed high activity towards pectin derived from soybean and pea.					
34489047	6	0	attach	derived	918:924	arg1	pea					943:945	pea	943:945	pea	943:945	When the substrate specificity was examined, AoPME1 showed high activity towards pectin derived from soybean and pea.					
34489047	12	1	theme	microbial	1695:1703	arg1	enzymes					1705:1711	microbial enzymes	1695:1711	microbial enzymes	1695:1711	This is the first report of this activity in microbial enzymes.					
34489047	11	2	theme	methoxy	1615:1621	arg1	group					1623:1627	the methoxy group	1611:1627	the methoxy group in xylogalacturonan	1611:1647	Thus, AoPME1 and AoPME3 hydrolyzed the methoxy group in xylogalacturonan.					
34489047	13	3	theme	food	1901:1904	arg1	manufacturing					1906:1918	food manufacturing	1901:1918	food manufacturing	1901:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	10	4	with	endo-polygalacturonase	1480:1501	arg1	AoPME2					1518:1523	AoPME2	1518:1523	AoPME2	1518:1523	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	10	4	with	endo-polygalacturonase	1480:1501	arg1	AoPME1					1508:1513	AoPME1	1508:1513	AoPME1	1508:1513	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	4	5	theme	optimal	617:623	arg1	temperatures					634:645	optimal reaction temperatures	617:645	optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6	617:698	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	13	6	theme	substrate	1734:1742	arg1	specificity					1744:1754	the substrate specificity	1730:1754	the substrate specificity of PMEs	1730:1762	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	7	theme	new	1868:1870	arg1	applications					1872:1883	new applications	1868:1883	new applications in the field of food manufacturing	1868:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	7	8	theme	citrus-derived	1058:1071	arg1	pectins					1073:1079	apple- and citrus-derived pectins	1047:1079	apple- and citrus-derived pectins	1047:1079	Although AoPME2 showed little activity towards these pectins, it showed very high activity towards apple- and citrus-derived pectins.					
34489047	9	9	from	rich	1229:1232	arg1	homogalacturonan					1237:1252	homogalacturonan	1237:1252	homogalacturonan	1237:1252	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	7	10	theme	little	971:976	arg1	activity					978:985	little activity	971:985	little activity towards these pectins	971:1007	Although AoPME2 showed little activity towards these pectins, it showed very high activity towards apple- and citrus-derived pectins.					
34489047	10	11	theme	pea	1330:1332	arg1	pectin					1334:1339	pea pectin	1330:1339	pea pectin	1330:1339	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	12	12	theme	activity	1683:1690	arg1	This					1650:1653	This	1650:1653	This	1650:1653	This is the first report of this activity in microbial enzymes.					
34489047	12	12	theme	activity	1683:1690	arg1	report					1668:1673	the first report	1658:1673	the first report of this activity in microbial enzymes	1658:1711	This is the first report of this activity in microbial enzymes.					
34489047	8	13	theme	specific	1100:1107	arg1	activity					1109:1116	low specific activity	1096:1116	low specific activity towards all substrates tested	1096:1146	AoPME3 showed low specific activity towards all substrates tested.					
34489047	3	14	attach	derived	421:427	arg2	family					412:417	Carbohydrate Esterase family 8	390:419	Carbohydrate Esterase family 8 derived from A. oryzae	390:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	3	14	attach	derived	421:427	arg1	oryzae					437:442	A. oryzae	434:442	A. oryzae	434:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	12	15	from	report	1668:1673	arg1	enzymes					1705:1711	microbial enzymes	1695:1711	microbial enzymes	1695:1711	This is the first report of this activity in microbial enzymes.					
34489047	13	16	from	findings	1718:1725	arg1	specificity					1744:1754	the substrate specificity	1730:1754	the substrate specificity of PMEs	1730:1762	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	2	17	theme	self-cloning	249:260	arg1	vector					262:267	a self-cloning vector	247:267	a self-cloning vector capable of high expression in A. oryzae	247:307	We constructed a self-cloning vector capable of high expression in A. oryzae.					
34489047	4	18	from	effects	528:534	arg1	enzymes					571:577	the three enzymes	561:577	the three enzymes	561:577	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	10	19	dep	observed	1470:1477	arg1	endo-polygalacturonase					1480:1501	endo-polygalacturonase	1480:1501	endo-polygalacturonase	1480:1501	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	10	19	dep	observed	1470:1477	arg1	endo-xylogalacturonase					1529:1550	endo-xylogalacturonase	1529:1550	endo-xylogalacturonase	1529:1550	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	9	20	theme	Sugar	1149:1153	arg1	analysis					1167:1174	Sugar composition analysis	1149:1174	Sugar composition analysis	1149:1174	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	1	21	theme	Aspergillus	147:157	arg1	oryzae					159:164	Aspergillus oryzae	147:164	Aspergillus oryzae	147:164	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	1	21	theme	Aspergillus	147:157	arg1	microorganism					176:188	a safe microorganism	169:188	a safe microorganism that is commonly used in food production	169:229	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	4	22	theme	reaction	673:680	arg1	range					685:689	optimal reaction pH range	665:689	optimal reaction pH range	665:689	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	2	23	theme	high	280:283	arg1	expression					285:294	high expression	280:294	high expression in A. oryzae	280:307	We constructed a self-cloning vector capable of high expression in A. oryzae.					
34489047	13	24	from	applications	1872:1883	arg1	field					1892:1896	the field	1888:1896	the field of food manufacturing	1888:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	3	25	theme	pectin	343:348	arg1	PME					366:368	PME	366:368	PME	366:368	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	3	25	theme	pectin	343:348	arg1	methylesterase					350:363	pectin methylesterase	343:363	three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae	328:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	4	26	theme	50	650:651	arg1	°C					658:659	50 - 60 °C	650:659	°C	658:659	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	0	27	theme	pectin	90:95	arg1	AoPME1-3					114:121	AoPME1-3	114:121	AoPME1-3	114:121	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	0	27	theme	pectin	90:95	arg1	methylesterases					97:111	self-cloning vector-expressed pectin methylesterases	60:111	self-cloning vector-expressed pectin methylesterases (AoPME1-3)	60:122	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	0	28	theme	xylogalacturonan	21:36	arg1	specificity					45:55	xylogalacturonan region specificity	21:55	xylogalacturonan region specificity	21:55	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	1	29	theme	safe	171:174	arg1	oryzae					159:164	Aspergillus oryzae	147:164	Aspergillus oryzae	147:164	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	1	29	theme	safe	171:174	arg1	microorganism					176:188	a safe microorganism	169:188	a safe microorganism that is commonly used in food production	169:229	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	6	30	theme	high	889:892	arg1	activity					894:901	high activity	889:901	high activity towards pectin derived from soybean and pea	889:945	When the substrate specificity was examined, AoPME1 showed high activity towards pectin derived from soybean and pea.					
34489047	4	31	theme	60	655:656	arg1	°C					658:659	50 - 60 °C	650:659	°C	658:659	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	13	32	theme	groups	1828:1833	arg1	distribution					1804:1815	the distribution	1800:1815	the distribution of methoxy groups in pectin	1800:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	0	33	from	Homogalacturonan	0:15	arg1	oryzae					139:144	Aspergillus oryzae	127:144	Aspergillus oryzae	127:144	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	4	34	theme	6	698:698	arg1	range					685:689	optimal reaction pH range	665:689	optimal reaction pH range	665:689	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	34	theme	6	698:698	arg1	°C					658:659	50 - 60 °C	650:659	°C	658:659	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	13	35	from	pectin	1838:1843	arg1	determination					1783:1795	the determination	1779:1795	the determination of the distribution of methoxy groups in pectin	1779:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	35	from	pectin	1838:1843	arg1	development					1853:1863	the development	1849:1863	the development of new applications in the field of food manufacturing	1849:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	0	36	theme	Aspergillus	127:137	arg1	oryzae					139:144	Aspergillus oryzae	127:144	Aspergillus oryzae	127:144	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	5	37	theme	specific	705:712	arg1	U/mg					809:812	34, 7,601, and 2 U/mg	792:812	34, 7,601, and 2 U/mg	792:812	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	37	theme	specific	705:712	arg1	different					781:789	different	781:789	different	781:789	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	37	theme	specific	705:712	arg1	activities					714:723	The specific activities	701:723	The specific activities of AoPME1, 2, and 3 for apple pectin	701:760	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	3	38	theme	Carbohydrate	390:401	arg1	family					412:417	Carbohydrate Esterase family 8	390:419	Carbohydrate Esterase family 8 derived from A. oryzae	390:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	0	39	from	specificity	45:55	arg1	oryzae					139:144	Aspergillus oryzae	127:144	Aspergillus oryzae	127:144	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	4	40	theme	range	685:689	arg1	temperatures					634:645	optimal reaction temperatures	617:645	optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6	617:698	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	5	41	theme	2	736:736	arg1	U/mg					809:812	34, 7,601, and 2 U/mg	792:812	34, 7,601, and 2 U/mg	792:812	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	41	theme	2	736:736	arg1	different					781:789	different	781:789	different	781:789	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	41	theme	2	736:736	arg1	activities					714:723	The specific activities	701:723	The specific activities of AoPME1, 2, and 3 for apple pectin	701:760	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	2	42	theme	capable	269:275	arg1	vector					262:267	a self-cloning vector	247:267	a self-cloning vector capable of high expression in A. oryzae	247:307	We constructed a self-cloning vector capable of high expression in A. oryzae.					
34489047	4	43	theme	pH	555:556	arg1	similar					603:609	similar	603:609	similar	603:609	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	43	theme	pH	555:556	arg1	AoPME1					580:585	AoPME1	580:585	AoPME1	580:585	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	43	theme	pH	555:556	arg1	effects					528:534	The effects	524:534	The effects of temperature and pH on the three enzymes	524:577	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	43	theme	pH	555:556	arg1	2					588:588	2	588:588	2	588:588	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	43	theme	pH	555:556	arg1	3					595:595	3	595:595	3	595:595	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	44	theme	5	694:694	arg1	range					685:689	optimal reaction pH range	665:689	optimal reaction pH range	665:689	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	44	theme	5	694:694	arg1	°C					658:659	50 - 60 °C	650:659	°C	658:659	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	3	45	theme	several	464:470	arg1	characteristics					472:486	several characteristics	464:486	several characteristics of the gene products	464:507	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	7	46	theme	apple-	1047:1052	arg1	pectins					1073:1079	apple- and citrus-derived pectins	1047:1079	apple- and citrus-derived pectins	1047:1079	Although AoPME2 showed little activity towards these pectins, it showed very high activity towards apple- and citrus-derived pectins.					
34489047	2	47	from	expression	285:294	arg1	A.					299:300	A.	299:300	A.	299:300	We constructed a self-cloning vector capable of high expression in A. oryzae.					
34489047	2	48	dep	A.	299:300	arg1	oryzae					302:307	A. oryzae	299:307	A. oryzae	299:307	We constructed a self-cloning vector capable of high expression in A. oryzae.					
34489047	5	49	dep	different	781:789	arg1	different					781:789	different	781:789	different	781:789	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	49	dep	different	781:789	arg1	U/mg					809:812	34, 7,601, and 2 U/mg	792:812	34, 7,601, and 2 U/mg	792:812	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	49	dep	different	781:789	arg1	activities					714:723	The specific activities	701:723	The specific activities of AoPME1, 2, and 3 for apple pectin	701:760	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	4	50	dep	similar	603:609	arg1	AoPME1					580:585	AoPME1	580:585	AoPME1	580:585	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	50	dep	similar	603:609	arg1	similar					603:609	similar	603:609	similar	603:609	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	50	dep	similar	603:609	arg1	effects					528:534	The effects	524:534	The effects of temperature and pH on the three enzymes	524:577	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	50	dep	similar	603:609	arg1	2					588:588	2	588:588	2	588:588	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	50	dep	similar	603:609	arg1	3					595:595	3	595:595	3	595:595	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	3	51	theme	gene	495:498	arg1	products					500:507	the gene products	491:507	the gene products	491:507	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	5	52	theme	AoPME1	728:733	arg1	U/mg					809:812	34, 7,601, and 2 U/mg	792:812	34, 7,601, and 2 U/mg	792:812	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	52	theme	AoPME1	728:733	arg1	different					781:789	different	781:789	different	781:789	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	52	theme	AoPME1	728:733	arg1	activities					714:723	The specific activities	701:723	The specific activities of AoPME1, 2, and 3 for apple pectin	701:760	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	4	53	theme	temperature	539:549	arg1	similar					603:609	similar	603:609	similar	603:609	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	53	theme	temperature	539:549	arg1	AoPME1					580:585	AoPME1	580:585	AoPME1	580:585	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	53	theme	temperature	539:549	arg1	effects					528:534	The effects	524:534	The effects of temperature and pH on the three enzymes	524:577	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	53	theme	temperature	539:549	arg1	2					588:588	2	588:588	2	588:588	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	4	53	theme	temperature	539:549	arg1	3					595:595	3	595:595	3	595:595	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	10	54	theme	synergistic	1445:1455	arg1	actions					1457:1463	specific synergistic actions	1436:1463	specific synergistic actions	1436:1463	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	4	55	with	similar	603:609	arg1	temperatures					634:645	optimal reaction temperatures	617:645	optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6	617:698	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	13	56	from	determination	1783:1795	arg1	pectin					1838:1843	pectin	1838:1843	pectin	1838:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	56	from	determination	1783:1795	arg1	field					1892:1896	the field	1888:1896	the field of food manufacturing	1888:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	57	theme	PMEs	1759:1762	arg1	specificity					1744:1754	the substrate specificity	1730:1754	the substrate specificity of PMEs	1730:1762	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	58	theme	manufacturing	1906:1918	arg1	field					1892:1896	the field	1888:1896	the field of food manufacturing	1888:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	0	59	theme	self-cloning	60:71	arg1	AoPME1-3					114:121	AoPME1-3	114:121	AoPME1-3	114:121	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	0	59	theme	self-cloning	60:71	arg1	methylesterases					97:111	self-cloning vector-expressed pectin methylesterases	60:111	self-cloning vector-expressed pectin methylesterases (AoPME1-3)	60:122	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	13	60	theme	applications	1872:1883	arg1	determination					1783:1795	the determination	1779:1795	the determination of the distribution of methoxy groups in pectin	1779:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	60	theme	applications	1872:1883	arg1	development					1853:1863	the development	1849:1863	the development of new applications in the field of food manufacturing	1849:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	4	61	theme	reaction	625:632	arg1	temperatures					634:645	optimal reaction temperatures	617:645	optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6	617:698	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	5	62	theme	3	743:743	arg1	U/mg					809:812	34, 7,601, and 2 U/mg	792:812	34, 7,601, and 2 U/mg	792:812	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	62	theme	3	743:743	arg1	different					781:789	different	781:789	different	781:789	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	5	62	theme	3	743:743	arg1	activities					714:723	The specific activities	701:723	The specific activities of AoPME1, 2, and 3 for apple pectin	701:760	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	6	63	theme	substrate	839:847	arg1	specificity					849:859	the substrate specificity	835:859	the substrate specificity	835:859	When the substrate specificity was examined, AoPME1 showed high activity towards pectin derived from soybean and pea.					
34489047	5	64	theme	apple	749:753	arg1	pectin					755:760	apple pectin	749:760	apple pectin	749:760	The specific activities of AoPME1, 2, and 3 for apple pectin were significantly different (34, 7,601, and 2 U/mg, respectively).					
34489047	8	65	theme	low	1096:1098	arg1	activity					1109:1116	low specific activity	1096:1116	low specific activity towards all substrates tested	1096:1146	AoPME3 showed low specific activity towards all substrates tested.					
34489047	4	66	theme	°C	658:659	arg1	temperatures					634:645	optimal reaction temperatures	617:645	optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6	617:698	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	9	67	theme	composition	1155:1165	arg1	analysis					1167:1174	Sugar composition analysis	1149:1174	Sugar composition analysis	1149:1174	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	2	68	theme	expression	285:294	arg1	capable					269:275	capable	269:275	capable	269:275	We constructed a self-cloning vector capable of high expression in A. oryzae.					
34489047	1	69	used	used	207:210	arg2	microorganism					176:188	a safe microorganism	169:188	a safe microorganism that is commonly used in food production	169:229	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	1	69	used	used	207:210	arg2	oryzae					159:164	Aspergillus oryzae	147:164	Aspergillus oryzae	147:164	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	4	70	theme	optimal	665:671	arg1	range					685:689	optimal reaction pH range	665:689	optimal reaction pH range	665:689	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	0	71	theme	methylesterases	97:111	arg1	Homogalacturonan					0:15	Homogalacturonan	0:15	Homogalacturonan	0:15	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	0	71	theme	methylesterases	97:111	arg1	specificity					45:55	xylogalacturonan region specificity	21:55	xylogalacturonan region specificity	21:55	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	1	72	theme	food	215:218	arg1	production					220:229	food production	215:229	food production	215:229	Aspergillus oryzae is a safe microorganism that is commonly used in food production.					
34489047	12	73	theme	first	1662:1666	arg1	This					1650:1653	This	1650:1653	This	1650:1653	This is the first report of this activity in microbial enzymes.					
34489047	12	73	theme	first	1662:1666	arg1	report					1668:1673	the first report	1658:1673	the first report of this activity in microbial enzymes	1658:1711	This is the first report of this activity in microbial enzymes.					
34489047	3	74	theme	methylesterase	350:363	arg1	genes					371:375	three putative pectin methylesterase (PME) genes	328:375	three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae	328:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	13	75	theme	methoxy	1820:1826	arg1	groups					1828:1833	methoxy groups	1820:1833	methoxy groups	1820:1833	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	9	76	theme	apple-	1190:1195	arg1	pectins					1216:1222	apple- and citrus-derived pectins	1190:1222	apple- and citrus-derived pectins	1190:1222	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	13	77	from	field	1892:1896	arg1	determination					1783:1795	the determination	1779:1795	the determination of the distribution of methoxy groups in pectin	1779:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	77	from	field	1892:1896	arg1	development					1853:1863	the development	1849:1863	the development of new applications in the field of food manufacturing	1849:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	3	78	theme	putative	334:341	arg1	genes					371:375	three putative pectin methylesterase (PME) genes	328:375	three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae	328:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	9	79	theme	citrus-derived	1201:1214	arg1	pectins					1216:1222	apple- and citrus-derived pectins	1190:1222	apple- and citrus-derived pectins	1190:1222	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	13	80	theme	distribution	1804:1815	arg1	determination					1783:1795	the determination	1779:1795	the determination of the distribution of methoxy groups in pectin	1779:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	80	theme	distribution	1804:1815	arg1	development					1853:1863	the development	1849:1863	the development of new applications in the field of food manufacturing	1849:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	9	81	from	homogalacturonan	1237:1252	arg1	rich					1229:1232	rich	1229:1232	rich	1229:1232	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	13	82	from	development	1853:1863	arg1	pectin					1838:1843	pectin	1838:1843	pectin	1838:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	13	82	from	development	1853:1863	arg1	field					1892:1896	the field	1888:1896	the field of food manufacturing	1888:1918	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	3	83	theme	Esterase	403:410	arg1	family					412:417	Carbohydrate Esterase family 8	390:419	Carbohydrate Esterase family 8 derived from A. oryzae	390:442	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	4	84	theme	pH	682:683	arg1	range					685:689	optimal reaction pH range	665:689	optimal reaction pH range	665:689	The effects of temperature and pH on the three enzymes (AoPME1, 2, and 3) were similar, with optimal reaction temperatures of 50 - 60 °C and optimal reaction pH range of 5 - 6.					
34489047	0	85	theme	region	38:43	arg1	specificity					45:55	xylogalacturonan region specificity	21:55	xylogalacturonan region specificity	21:55	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	9	86	link	citrus-derived	1201:1214	arg1	pectins					1216:1222	apple- and citrus-derived pectins	1190:1222	apple- and citrus-derived pectins	1190:1222	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	11	87	from	group	1623:1627	arg1	xylogalacturonan					1632:1647	xylogalacturonan	1632:1647	xylogalacturonan	1632:1647	Thus, AoPME1 and AoPME3 hydrolyzed the methoxy group in xylogalacturonan.					
34489047	10	88	theme	PME	1431:1433	arg1	presence					1414:1421	the presence	1410:1421	the presence of each PME	1410:1433	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	7	89	theme	high	1025:1028	arg1	activity					1030:1037	very high activity	1020:1037	very high activity towards apple- and citrus-derived pectins	1020:1079	Although AoPME2 showed little activity towards these pectins, it showed very high activity towards apple- and citrus-derived pectins.					
34489047	9	90	theme	soybean-	1261:1268	arg1	pectins					1286:1292	soybean- and pea-derived pectins	1261:1292	soybean- and pea-derived pectins	1261:1292	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	0	91	theme	vector-expressed	73:88	arg1	AoPME1-3					114:121	AoPME1-3	114:121	AoPME1-3	114:121	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	0	91	theme	vector-expressed	73:88	arg1	methylesterases					97:111	self-cloning vector-expressed pectin methylesterases	60:111	self-cloning vector-expressed pectin methylesterases (AoPME1-3)	60:122	Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1-3) in Aspergillus oryzae.					
34489047	9	92	theme	pea-derived	1274:1284	arg1	pectins					1286:1292	soybean- and pea-derived pectins	1261:1292	soybean- and pea-derived pectins	1261:1292	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	9	93	from	xylogalacturonan	1307:1322	arg1	rich					1299:1302	rich	1299:1302	rich	1299:1302	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	10	94	with	endo-xylogalacturonase	1529:1550	arg1	AoPME1					1557:1562	AoPME1	1557:1562	AoPME1	1557:1562	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	10	94	with	endo-xylogalacturonase	1529:1550	arg1	AoPME3					1567:1572	AoPME3	1567:1572	AoPME3	1567:1572	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	3	95	theme	products	500:507	arg1	characteristics					472:486	several characteristics	464:486	several characteristics of the gene products	464:507	Using the vector, three putative pectin methylesterase (PME) genes belonging to Carbohydrate Esterase family 8 derived from A. oryzae were expressed, and several characteristics of the gene products were examined.					
34489047	9	96	from	rich	1299:1302	arg1	xylogalacturonan					1307:1322	xylogalacturonan	1307:1322	xylogalacturonan	1307:1322	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	13	97	from	distribution	1804:1815	arg1	pectin					1838:1843	pectin	1838:1843	pectin	1838:1843	Our findings on the substrate specificity of PMEs should lead to the determination of the distribution of methoxy groups in pectin and the development of new applications in the field of food manufacturing.					
34489047	9	98	link	pea-derived	1274:1284	arg1	pectins					1286:1292	soybean- and pea-derived pectins	1261:1292	soybean- and pea-derived pectins	1261:1292	Sugar composition analysis revealed that apple- and citrus-derived pectins were rich in homogalacturonan, while soybean- and pea-derived pectins were rich in xylogalacturonan.					
34489047	10	99	theme	specific	1436:1443	arg1	actions					1457:1463	specific synergistic actions	1436:1463	specific synergistic actions	1436:1463	When pea pectin was treated with endo-polygalacturonase or endo-xylogalacturonase in the presence of each PME, specific synergistic actions were observed (endo-polygalacturonase with AoPME1 or AoPME2 and endo-xylogalacturonase with AoPME1 or AoPME3).					
34489047	7	100	link	citrus-derived	1058:1071	arg1	pectins					1073:1079	apple- and citrus-derived pectins	1047:1079	apple- and citrus-derived pectins	1047:1079	Although AoPME2 showed little activity towards these pectins, it showed very high activity towards apple- and citrus-derived pectins.					
32128732	0	0	theme	alginate	85:92	arg1	extraction					94:103	alginate extraction	85:103	alginate extraction from Sargassum filipendula algae	85:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	6	1	theme	sieving	957:963	arg1	effects					965:971	Molecular sieving effects	947:971	Molecular sieving effects	947:971	Molecular sieving effects were excluded by assessing the molecular geometry of propranolol.					
32128732	2	2	theme	aqueous	384:390	arg1	media					392:396	aqueous media	384:396	aqueous media	384:396	In this study, biosorption is presented as an advanced approach for propranolol uptake from aqueous media.					
32128732	4	3	theme	%	585:585	arg1	efficiency					595:604	The high 93% removal efficiency	574:604	The high 93% removal efficiency achieved	574:613	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	6	4	theme	Molecular	947:955	arg1	effects					965:971	Molecular sieving effects	947:971	Molecular sieving effects	947:971	Molecular sieving effects were excluded by assessing the molecular geometry of propranolol.					
32128732	5	5	theme	propranolol	922:932	arg1	biosorption					934:944	propranolol biosorption	922:944	propranolol biosorption	922:944	RSF had morphology, porosity, chemical composition, and thermal behavior characterized prior and post to application in propranolol biosorption.					
32128732	1	6	theme	anti-hypertensive	178:194	arg1	hydrochloride					151:163	Propranolol hydrochloride	139:163	Propranolol hydrochloride	139:163	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	1	6	theme	anti-hypertensive	178:194	arg1	pollutant					200:208	a popular anti-hypertensive and pollutant	168:208	a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment	168:289	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	4	7	theme	93	583:584	arg1	%					585:585	%	585:585	%	585:585	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	3	8	theme	hydroxyl	546:553	arg1	sites					519:523	its key binding sites	503:523	its key binding sites	503:523	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	8	theme	hydroxyl	546:553	arg1	groups					566:571	carboxyl and hydroxyl functional groups	533:571	groups	566:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	9	theme	functional	555:564	arg1	sites					519:523	its key binding sites	503:523	its key binding sites	503:523	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	9	theme	functional	555:564	arg1	groups					566:571	carboxyl and hydroxyl functional groups	533:571	groups	566:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	0	10	theme	extraction	94:103	arg1	biomass					74:80	remaining biomass	64:80	remaining biomass of alginate extraction from Sargassum filipendula algae	64:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	0	11	dep	Sargassum	110:118	arg1	algae					132:136	Sargassum filipendula algae	110:136	Sargassum filipendula algae	110:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	5	12	from	application	907:917	arg1	biosorption					934:944	propranolol biosorption	922:944	propranolol biosorption	922:944	RSF had morphology, porosity, chemical composition, and thermal behavior characterized prior and post to application in propranolol biosorption.					
32128732	3	13	theme	binding	511:517	arg1	sites					519:523	its key binding sites	503:523	its key binding sites	503:523	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	13	theme	binding	511:517	arg1	groups					566:571	carboxyl and hydroxyl functional groups	533:571	groups	566:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	4	14	theme	removal	587:593	arg1	efficiency					595:604	The high 93% removal efficiency	574:604	The high 93% removal efficiency achieved	574:613	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	5	15	theme	thermal	858:864	arg1	behavior					866:873	thermal behavior	858:873	thermal behavior	858:873	RSF had morphology, porosity, chemical composition, and thermal behavior characterized prior and post to application in propranolol biosorption.					
32128732	3	16	theme	carboxyl	533:540	arg1	sites					519:523	its key binding sites	503:523	its key binding sites	503:523	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	16	theme	carboxyl	533:540	arg1	groups					566:571	carboxyl and hydroxyl functional groups	533:571	groups	566:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	6	17	theme	propranolol	1026:1036	arg1	geometry					1014:1021	the molecular geometry	1000:1021	the molecular geometry of propranolol	1000:1036	Molecular sieving effects were excluded by assessing the molecular geometry of propranolol.					
32128732	3	18	from	biomass	413:419	arg1	RSF					464:466	RSF	464:466	RSF	464:466	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	18	from	biomass	413:419	arg1	seaweed					455:461	seaweed	455:461	seaweed	455:461	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	9	19	theme	thermodynamic	1265:1277	arg1	parameters					1279:1288	thermodynamic parameters	1265:1288	thermodynamic parameters	1265:1288	Propranolol biosorption onto RSF was further inspected by thermodynamic parameters, including isosteric heat.					
32128732	9	19	theme	thermodynamic	1265:1277	arg1	heat					1311:1314	isosteric heat	1301:1314	isosteric heat	1301:1314	Propranolol biosorption onto RSF was further inspected by thermodynamic parameters, including isosteric heat.					
32128732	4	20	theme	environmental	672:684	arg1	remediation					686:696	propranolol environmental remediation	660:696	propranolol environmental remediation	660:696	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	8	21	theme	Langmuir	1110:1117	arg1	equations					1157:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations	1110:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations	1110:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations were tested for experimental isotherms.					
32128732	2	22	theme	advanced	338:345	arg1	biosorption					307:317	biosorption	307:317	biosorption	307:317	In this study, biosorption is presented as an advanced approach for propranolol uptake from aqueous media.					
32128732	2	22	theme	advanced	338:345	arg1	approach					347:354	an advanced approach	335:354	an advanced approach for propranolol uptake from aqueous media	335:396	In this study, biosorption is presented as an advanced approach for propranolol uptake from aqueous media.					
32128732	8	23	theme	Dubinin-Radushkevich	1136:1155	arg1	equations					1157:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations	1110:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations	1110:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations were tested for experimental isotherms.					
32128732	6	24	theme	molecular	1004:1012	arg1	geometry					1014:1021	the molecular geometry	1000:1021	the molecular geometry of propranolol	1000:1036	Molecular sieving effects were excluded by assessing the molecular geometry of propranolol.					
32128732	5	25	theme	chemical	832:839	arg1	composition					841:851	chemical composition	832:851	chemical composition	832:851	RSF had morphology, porosity, chemical composition, and thermal behavior characterized prior and post to application in propranolol biosorption.					
32128732	1	26	dep	anti-hypertensive	178:194	arg1	popular					170:176	popular	170:176	popular	170:176	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	0	27	theme	hydrochloride	23:35	arg1	Removal					0:6	Removal	0:6	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.	0:137	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	9	28	theme	Propranolol	1207:1217	arg1	biosorption					1219:1229	Propranolol biosorption	1207:1229	Propranolol biosorption onto RSF	1207:1238	Propranolol biosorption onto RSF was further inspected by thermodynamic parameters, including isosteric heat.					
32128732	8	29	theme	Freundlich	1120:1129	arg1	equations					1157:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations	1110:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations	1110:1165	Langmuir, Freundlich, and Dubinin-Radushkevich equations were tested for experimental isotherms.					
32128732	2	30	from	media	392:396	arg1	uptake					372:377	propranolol uptake	360:377	propranolol uptake from aqueous media	360:396	In this study, biosorption is presented as an advanced approach for propranolol uptake from aqueous media.					
32128732	0	31	theme	propranolol	11:21	arg1	hydrochloride					23:35	propranolol hydrochloride	11:35	propranolol hydrochloride	11:35	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	4	32	theme	high	578:581	arg1	efficiency					595:604	The high 93% removal efficiency	574:604	The high 93% removal efficiency achieved	574:613	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	9	33	theme	isosteric	1301:1309	arg1	heat					1311:1314	isosteric heat	1301:1314	isosteric heat	1301:1314	Propranolol biosorption onto RSF was further inspected by thermodynamic parameters, including isosteric heat.					
32128732	4	34	theme	effective	635:643	arg1	biosorbent					645:654	effective biosorbent	635:654	effective biosorbent for propranolol environmental remediation	635:696	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	0	35	theme	batch	40:44	arg1	biosorption					46:56	batch biosorption	40:56	batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae	40:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	3	36	theme	key	507:509	arg1	sites					519:523	its key binding sites	503:523	its key binding sites	503:523	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	36	theme	key	507:509	arg1	groups					566:571	carboxyl and hydroxyl functional groups	533:571	groups	566:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	7	37	theme	transfer	1093:1100	arg1	models					1102:1107	mass transfer models	1088:1107	mass transfer models	1088:1107	The kinetics was inspected by both rate laws and mass transfer models.					
32128732	4	38	theme	propranolol	660:670	arg1	remediation					686:696	propranolol environmental remediation	660:696	propranolol environmental remediation	660:696	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	0	39	from	biomass	74:80	arg1	Sargassum					110:118	Sargassum	110:118	Sargassum	110:118	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	1	40	theme	potential	241:249	arg1	risks					262:266	potential ecological risks	241:266	potential ecological risks to aquatic environment	241:289	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	3	41	theme	alginate	424:431	arg1	extraction					433:442	alginate extraction	424:442	alginate extraction from brown seaweed (RSF)	424:467	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	4	42	theme	alginate	781:788	arg1	extraction					790:799	alginate extraction	781:799	alginate extraction	781:799	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	3	43	theme	extraction	433:442	arg1	biomass					413:419	The remaining biomass	399:419	The remaining biomass of alginate extraction from brown seaweed (RSF)	399:467	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	43	theme	extraction	433:442	arg1	biosorbent					483:492	biosorbent	483:492	biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups	483:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	7	44	theme	mass	1088:1091	arg1	models					1102:1107	mass transfer models	1088:1107	mass transfer models	1088:1107	The kinetics was inspected by both rate laws and mass transfer models.					
32128732	2	45	theme	propranolol	360:370	arg1	uptake					372:377	propranolol uptake	360:377	propranolol uptake from aqueous media	360:396	In this study, biosorption is presented as an advanced approach for propranolol uptake from aqueous media.					
32128732	7	46	theme	rate	1074:1077	arg1	laws					1079:1082	rate laws	1074:1082	rate laws	1074:1082	The kinetics was inspected by both rate laws and mass transfer models.					
32128732	0	47	from	Sargassum	110:118	arg1	biomass					74:80	remaining biomass	64:80	remaining biomass of alginate extraction from Sargassum filipendula algae	64:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	0	47	from	Sargassum	110:118	arg1	extraction					94:103	alginate extraction	85:103	alginate extraction from Sargassum filipendula algae	85:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	1	48	theme	Propranolol	139:149	arg1	hydrochloride					151:163	Propranolol hydrochloride	139:163	Propranolol hydrochloride	139:163	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	1	48	theme	Propranolol	139:149	arg1	pollutant					200:208	a popular anti-hypertensive and pollutant	168:208	a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment	168:289	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	1	49	theme	ecological	251:260	arg1	risks					262:266	potential ecological risks	241:266	potential ecological risks to aquatic environment	241:289	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
32128732	3	50	from	seaweed	455:461	arg1	biomass					413:419	The remaining biomass	399:419	The remaining biomass of alginate extraction from brown seaweed (RSF)	399:467	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	50	from	seaweed	455:461	arg1	extraction					433:442	alginate extraction	424:442	alginate extraction from brown seaweed (RSF)	424:467	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	50	from	seaweed	455:461	arg1	biosorbent					483:492	biosorbent	483:492	biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups	483:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	8	51	theme	experimental	1183:1194	arg1	isotherms					1196:1204	experimental isotherms	1183:1204	experimental isotherms	1183:1204	Langmuir, Freundlich, and Dubinin-Radushkevich equations were tested for experimental isotherms.					
32128732	4	52	theme	waste	714:718	arg1	material					720:727	this waste material	709:727	this waste material	709:727	The high 93% removal efficiency achieved consolidates RSF as effective biosorbent for propranolol environmental remediation and values this waste material, which has been largely discarded in industry after alginate extraction.					
32128732	0	53	theme	remaining	64:72	arg1	biomass					74:80	remaining biomass	64:80	remaining biomass of alginate extraction from Sargassum filipendula algae	64:136	Removal of propranolol hydrochloride by batch biosorption using remaining biomass of alginate extraction from Sargassum filipendula algae.					
32128732	3	54	theme	remaining	403:411	arg1	biomass					413:419	The remaining biomass	399:419	The remaining biomass of alginate extraction from brown seaweed (RSF)	399:467	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	3	54	theme	remaining	403:411	arg1	biosorbent					483:492	biosorbent	483:492	biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups	483:571	The remaining biomass of alginate extraction from brown seaweed (RSF) was tested as biosorbent owing to its key binding sites, namely carboxyl and hydroxyl functional groups.					
32128732	1	55	theme	aquatic	271:277	arg1	environment					279:289	aquatic environment	271:289	aquatic environment	271:289	Propranolol hydrochloride is a popular anti-hypertensive and pollutant of emerging concern because of potential ecological risks to aquatic environment.					
31854933	0	0	theme	Wastewater	82:91	arg1	Treatment					93:101	a Wastewater Treatment	80:101	a Wastewater Treatment Containing Starch	80:119	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	1	1	theme	community	359:367	arg1	structure					369:377	the microbial community structure	345:377	the microbial community structure	345:377	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	1	2	from	composition	181:191	arg1	systems					244:250	anoxic/oxic (A/O) treatment systems	216:250	anoxic/oxic (A/O) treatment systems	216:250	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	3	3	theme	occupied	919:926	arg1	%					940:940	occupied 36.38%-66.65%	919:940	occupied 36.38%-66.65%	919:940	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	3	theme	occupied	919:926	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	3	theme	occupied	919:926	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	3	theme	occupied	919:926	arg1	%					861:861	45.66%-66.30%	849:861	45.66%-66.30%	849:861	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	2	4	theme	sludge	530:535	arg1	structure					507:515	the microbial community structure	483:515	the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems	483:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	4	theme	sludge	530:535	arg1	stable					605:610	stable	605:610	stable	605:610	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	1	5	from	community	157:165	arg1	systems					244:250	anoxic/oxic (A/O) treatment systems	216:250	anoxic/oxic (A/O) treatment systems	216:250	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	3	6	theme	main	903:906	arg1	member					908:913	the main member	899:913	the main member	899:913	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	6	theme	main	903:906	arg1	bacteria					885:892	γ-subclass bacteria	874:892	γ-subclass bacteria	874:892	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	1	7	theme	different	255:263	arg1	stages					275:280	different operation stages	255:280	different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment	255:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	2	8	theme	A/O	583:585	arg1	systems					587:593	A/O systems	583:593	A/O systems	583:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	0	9	from	[Measurements	0:12	arg1	Sludge					69:74	Activated Sludge	59:74	Activated Sludge	59:74	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	2	10	theme	activated	520:528	arg1	sludge					530:535	activated sludge	520:535	activated sludge	520:535	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	11	theme	sludge	697:702	arg1	bacteria					671:678	the dominant bacteria	658:678	the dominant bacteria of the activated sludge	658:702	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	11	theme	sludge	697:702	arg1	Proteobacteria					709:722	Proteobacteria	709:722	Proteobacteria	709:722	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	4	12	theme	sludge	1176:1181	arg1	better					1213:1218	better	1213:1218	better	1213:1218	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	12	theme	sludge	1176:1181	arg1	performance					1197:1207	the sludge sedimentation performance	1172:1207	the sludge sedimentation performance	1172:1207	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	1	13	theme	operation	265:273	arg1	stages					275:280	different operation stages	255:280	different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment	255:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	4	14	theme	Sphingobacteria	961:975	arg1	proportion					947:956	The proportion	943:956	The proportion of Sphingobacteria, the main member of the Bacteroidetes,	943:1014	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	6	15	from	sludge	1372:1377	arg1	number					1325:1330	a large number	1317:1330	a large number	1317:1330	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	2	16	theme	activated	687:695	arg1	sludge					697:702	the activated sludge	683:702	the activated sludge	683:702	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	4	17	theme	main	982:985	arg1	Sphingobacteria					961:975	Sphingobacteria	961:975	Sphingobacteria	961:975	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	17	theme	main	982:985	arg1	member					987:992	the main member	978:992	the main member of the Bacteroidetes	978:1013	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	18	theme	Chloroflexi	1130:1140	arg1	member					1120:1125	the main member	1111:1125	the main member of Chloroflexi	1111:1140	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	18	theme	Chloroflexi	1130:1140	arg1	Anaerolineae					1097:1108	Anaerolineae	1097:1108	Anaerolineae	1097:1108	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	1	19	theme	diversity	171:179	arg1	composition					181:191	the microbial community and diversity composition	143:191	composition	181:191	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	2	20	theme	wastewater	559:568	arg1	treatment					570:578	starch production wastewater treatment	541:578	starch production wastewater treatment in A/O systems	541:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	1	21	theme	activated	396:404	arg1	sludge					406:411	activated sludge	396:411	activated sludge	396:411	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	1	22	from	structure	369:377	arg1	sludge					406:411	activated sludge	396:411	activated sludge	396:411	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	3	23	theme	36.38	928:932	arg1	%					940:940	occupied 36.38%-66.65%	919:940	occupied 36.38%-66.65%	919:940	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	23	theme	36.38	928:932	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	23	theme	36.38	928:932	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	23	theme	36.38	928:932	arg1	%					861:861	45.66%-66.30%	849:861	45.66%-66.30%	849:861	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	2	24	theme	microbial	487:495	arg1	structure					507:515	the microbial community structure	483:515	the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems	483:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	24	theme	microbial	487:495	arg1	stable					605:610	stable	605:610	stable	605:610	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	3	25	theme	important	794:802	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	25	theme	important	794:802	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	2	26	theme	dominant	662:669	arg1	bacteria					671:678	the dominant bacteria	658:678	the dominant bacteria of the activated sludge	658:702	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	26	theme	dominant	662:669	arg1	Proteobacteria					709:722	Proteobacteria	709:722	Proteobacteria	709:722	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	6	27	from	bacteria	1346:1353	arg1	sludge					1372:1377	the activated sludge	1358:1377	the activated sludge	1358:1377	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	3	28	theme	%	933:933	arg1	%					940:940	occupied 36.38%-66.65%	919:940	occupied 36.38%-66.65%	919:940	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	28	theme	%	933:933	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	28	theme	%	933:933	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	28	theme	%	933:933	arg1	%					861:861	45.66%-66.30%	849:861	45.66%-66.30%	849:861	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	29	theme	dominant	804:811	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	29	theme	dominant	804:811	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	4	30	theme	main	1115:1118	arg1	member					1120:1125	the main member	1111:1125	the main member of Chloroflexi	1111:1140	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	30	theme	main	1115:1118	arg1	Anaerolineae					1097:1108	Anaerolineae	1097:1108	Anaerolineae	1097:1108	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	1	31	theme	activated	196:204	arg1	sludge					206:211	activated sludge	196:211	activated sludge	196:211	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	0	32	theme	Community	27:35	arg1	[Measurements					0:12	[Measurements	0:12	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch	0:119	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	1	33	theme	Illumina	288:295	arg1	sequencing					319:328	Illumina MiSeq high-throughput sequencing	288:328	Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment	288:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	4	34	theme	Bacteroidetes	1001:1013	arg1	Sphingobacteria					961:975	Sphingobacteria	961:975	Sphingobacteria	961:975	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	34	theme	Bacteroidetes	1001:1013	arg1	member					987:992	the main member	978:992	the main member of the Bacteroidetes	978:1013	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	1	35	theme	sludge	206:211	arg1	community					157:165	the microbial community and diversity composition	143:191	community	157:165	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	1	35	theme	sludge	206:211	arg1	composition					181:191	the microbial community and diversity composition	143:191	composition	181:191	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	0	36	theme	Bacterial	17:25	arg1	Community					27:35	Bacterial Community	17:35	Bacterial Community	17:35	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	1	37	theme	MiSeq	297:301	arg1	sequencing					319:328	Illumina MiSeq high-throughput sequencing	288:328	Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment	288:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	2	38	theme	experimental	450:461	arg1	results					463:469	The experimental results	446:469	The experimental results	446:469	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	0	39	theme	Biodiversity	41:52	arg1	[Measurements					0:12	[Measurements	0:12	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch	0:119	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	1	40	theme	high-throughput	303:317	arg1	sequencing					319:328	Illumina MiSeq high-throughput sequencing	288:328	Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment	288:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	6	41	theme	bacteria	1346:1353	arg1	number					1325:1330	a large number	1317:1330	a large number	1317:1330	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	1	42	theme	starch	417:422	arg1	treatment					435:443	starch wastewater treatment	417:443	starch wastewater treatment	417:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	1	43	theme	anoxic/oxic	216:226	arg1	systems					244:250	anoxic/oxic (A/O) treatment systems	216:250	anoxic/oxic (A/O) treatment systems	216:250	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	3	44	theme	bacterial	813:821	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	44	theme	bacterial	813:821	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	45	theme	Proteobacteria	833:846	arg1	%					861:861	45.66%-66.30%	849:861	45.66%-66.30%	849:861	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	45	theme	Proteobacteria	833:846	arg1	bacteria					885:892	γ-subclass bacteria	874:892	γ-subclass bacteria	874:892	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	45	theme	Proteobacteria	833:846	arg1	group					823:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group	785:827	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	45	theme	Proteobacteria	833:846	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	45	theme	Proteobacteria	833:846	arg1	%					940:940	occupied 36.38%-66.65%	919:940	occupied 36.38%-66.65%	919:940	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	45	theme	Proteobacteria	833:846	arg1	member					908:913	the main member	899:913	the main member	899:913	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	6	46	theme	functional	1335:1344	arg1	bacteria					1346:1353	functional bacteria	1335:1353	functional bacteria in the activated sludge	1335:1377	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	1	47	theme	wastewater	424:433	arg1	treatment					435:443	starch wastewater treatment	417:443	starch wastewater treatment	417:443	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	1	48	from	diversity	383:391	arg1	sludge					406:411	activated sludge	396:411	activated sludge	396:411	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	6	49	theme	activated	1362:1370	arg1	sludge					1372:1377	the activated sludge	1358:1377	the activated sludge	1358:1377	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	0	50	theme	Activated	59:67	arg1	Sludge					69:74	Activated Sludge	59:74	Activated Sludge	59:74	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	2	51	theme	wastewater	627:636	arg1	condition					638:646	the same wastewater condition	618:646	the same wastewater condition	618:646	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	0	52	from	Sludge	69:74	arg1	Biodiversity					41:52	Biodiversity	41:52	Biodiversity	41:52	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	0	52	from	Sludge	69:74	arg1	[Measurements					0:12	[Measurements	0:12	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch	0:119	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	0	52	from	Sludge	69:74	arg1	Community					27:35	Bacterial Community	17:35	Bacterial Community	17:35	[Measurements of Bacterial Community and Biodiversity from Activated Sludge for a Wastewater Treatment Containing Starch].					
31854933	6	53	dep	nitrogen	1451:1458	arg1	removal					1478:1484	removal	1478:1484	removal	1478:1484	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	2	54	theme	community	497:505	arg1	structure					507:515	the microbial community structure	483:515	the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems	483:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	54	theme	community	497:505	arg1	stable					605:610	stable	605:610	stable	605:610	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	55	theme	same	622:625	arg1	condition					638:646	the same wastewater condition	618:646	the same wastewater condition	618:646	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	2	56	from	treatment	570:578	arg1	systems					587:593	A/O systems	583:593	A/O systems	583:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	5	57	theme	settleability	1291:1303	arg1	behavior					1272:1279	the behavior	1268:1279	the behavior of sludge settleability	1268:1303	These changes may have been closely related to the behavior of sludge settleability.					
31854933	6	58	from	number	1325:1330	arg1	sludge					1372:1377	the activated sludge	1358:1377	the activated sludge	1358:1377	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	6	59	theme	large	1319:1323	arg1	number					1325:1330	a large number	1317:1330	a large number	1317:1330	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	1	60	dep	anoxic/oxic	216:226	arg1	A/O					229:231	A/O	229:231	A/O	229:231	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	2	61	dep	Proteobacteria	709:722	arg1	Bacteroidetes					725:737	Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria	709:782	Bacteroidetes	725:737	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	3	62	theme	γ-subclass	874:883	arg1	member					908:913	the main member	899:913	the main member	899:913	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	62	theme	γ-subclass	874:883	arg1	bacteria					885:892	γ-subclass bacteria	874:892	γ-subclass bacteria	874:892	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	2	63	theme	production	548:557	arg1	treatment					570:578	starch production wastewater treatment	541:578	starch production wastewater treatment in A/O systems	541:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	5	64	theme	sludge	1284:1289	arg1	settleability					1291:1303	sludge settleability	1284:1303	sludge settleability	1284:1303	These changes may have been closely related to the behavior of sludge settleability.					
31854933	6	65	theme	important	1396:1404	arg1	role					1406:1409	an important role	1393:1409	an important role	1393:1409	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	4	66	theme	Anaerolineae	1097:1108	arg1	proportion					1083:1092	the proportion	1079:1092	the proportion of Anaerolineae, the main member of Chloroflexi,	1079:1141	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	1	67	theme	treatment	234:242	arg1	systems					244:250	anoxic/oxic (A/O) treatment systems	216:250	anoxic/oxic (A/O) treatment systems	216:250	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	2	68	theme	starch	541:546	arg1	treatment					570:578	starch production wastewater treatment	541:578	starch production wastewater treatment in A/O systems	541:593	The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria.					
31854933	1	69	theme	microbial	349:357	arg1	structure					369:377	the microbial community structure	345:377	the microbial community structure	345:377	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	6	70	theme	pollutants	1433:1442	arg1	degradation					1418:1428	the degradation	1414:1428	the degradation of pollutants	1414:1442	There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal.					
31854933	4	71	theme	sedimentation	1183:1195	arg1	better					1213:1218	better	1213:1218	better	1213:1218	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	4	71	theme	sedimentation	1183:1195	arg1	performance					1197:1207	the sludge sedimentation performance	1172:1207	the sludge sedimentation performance	1172:1207	The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better.					
31854933	1	72	theme	microbial	147:155	arg1	community					157:165	the microbial community and diversity composition	143:191	community	157:165	This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment.					
31854933	3	73	theme	%	854:854	arg1	%					861:861	45.66%-66.30%	849:861	45.66%-66.30%	849:861	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
31854933	3	73	theme	%	854:854	arg1	Proteobacteria					833:846	Proteobacteria	833:846	Proteobacteria (45.66%-66.30%)	833:862	The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%.					
36001489	9	0	theme	porcine	1279:1285	arg1	pancreata					1287:1295	the porcine pancreata	1275:1295	the porcine pancreata	1275:1295	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	2	1	theme	MK	593:594	arg1	solution					596:603	MK solution	593:603	MK solution	593:603	In this study, we evaluated the efficiency of a modified histidine-lactobionate (MHL) solution in addition to UW or MK solution.					
36001489	9	2	from	outcome	1326:1332	arg1	mice					1394:1397	streptozotocin-induced diabetic mice	1362:1397	streptozotocin-induced diabetic mice	1362:1397	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	9	3	theme	transplantation	1343:1357	arg1	outcome					1326:1332	the outcome	1322:1332	the outcome of islet transplantation in streptozotocin-induced diabetic mice	1322:1397	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	7	4	theme	islet	1032:1036	arg1	yield					1038:1042	The islet yield	1028:1042	The islet yield before and after purification	1028:1072	RESULTS The islet yield before and after purification was significantly higher in the MHL group than in the UW group.					
36001489	7	4	theme	islet	1032:1036	arg1	higher					1092:1097	higher	1092:1097	higher	1092:1097	RESULTS The islet yield before and after purification was significantly higher in the MHL group than in the UW group.					
36001489	0	5	from	Preservation	9:20	arg1	Solution					57:64	Modified Histidine-lactobionate Solution	25:64	Modified Histidine-lactobionate Solution	25:64	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	3	6	theme	sodium-low	634:643	arg1	composition					655:665	a high sodium-low potassium composition	627:665	a high sodium-low potassium composition with low viscosity	627:684	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	1	7	theme	organ	437:441	arg1	preservation					443:454	organ preservation	437:454	organ preservation for islet isolation	437:474	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	3	8	theme	low	672:674	arg1	viscosity					676:684	low viscosity	672:684	low viscosity	672:684	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	1	9	theme	trypsin	277:283	arg1	ulinastatin					296:306	ulinastatin	296:306	ulinastatin	296:306	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	1	9	theme	trypsin	277:283	arg1	inhibitor					285:293	a trypsin inhibitor	275:293	a trypsin inhibitor (ulinastatin)	275:307	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	0	10	theme	Wisconsin	103:111	arg1	Solution					113:120	Wisconsin Solution	103:120	Wisconsin Solution for Porcine Islet Isolation	103:148	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	10	11	theme	MHL	1473:1475	arg1	solution					1477:1484	MHL solution	1473:1484	MHL solution	1473:1484	CONCLUSIONS Pancreas preservation with MHL solution preserves islet function better than UW solution.					
36001489	6	12	theme	islets	931:936	arg1	number					902:907	An optimized number	889:907	An optimized number (1500 IE) of isolated islets from each group	889:952	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	6	12	theme	islets	931:936	arg1	IE					915:916	1500 IE	910:916	1500 IE	910:916	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	1	13	theme	Wisconsin	377:385	arg1	gold					419:422	the gold	415:422	the gold standard for organ preservation for islet isolation	415:474	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	1	13	theme	Wisconsin	377:385	arg1	preservation					392:403	Wisconsin (UW) preservation	377:403	Wisconsin (UW) preservation	377:403	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	6	14	theme	optimized	892:900	arg1	number					902:907	An optimized number	889:907	An optimized number (1500 IE) of isolated islets from each group	889:952	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	6	14	theme	optimized	892:900	arg1	IE					915:916	1500 IE	910:916	1500 IE	910:916	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	4	15	theme	MK	738:739	arg1	solution					741:748	MK solution	738:748	MK solution	738:748	Moreover, similar to MK solution, MHL solution also contains ulinastatin.					
36001489	6	16	theme	isolated	922:929	arg1	islets					931:936	isolated islets	922:936	isolated islets from each group	922:952	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	1	17	theme	islet	460:464	arg1	isolation					466:474	islet isolation	460:474	islet isolation	460:474	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	4	18	theme	MHL	751:753	arg1	solution					755:762	MHL solution	751:762	MHL solution	751:762	Moreover, similar to MK solution, MHL solution also contains ulinastatin.					
36001489	9	19	theme	diabetic	1385:1392	arg1	mice					1394:1397	streptozotocin-induced diabetic mice	1362:1397	streptozotocin-induced diabetic mice	1362:1397	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	1	20	theme	UW	388:389	arg1	gold					419:422	the gold	415:422	the gold standard for organ preservation for islet isolation	415:474	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	1	20	theme	UW	388:389	arg1	preservation					392:403	Wisconsin (UW) preservation	377:403	Wisconsin (UW) preservation	377:403	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	7	21	theme	MHL	1106:1108	arg1	group					1110:1114	the MHL group	1102:1114	the MHL group	1102:1114	RESULTS The islet yield before and after purification was significantly higher in the MHL group than in the UW group.					
36001489	5	22	theme	METHODS	791:797	arg1	pancreata					807:815	METHODS Porcine pancreata	791:815	METHODS Porcine pancreata	791:815	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	3	23	with	composition	655:665	arg1	viscosity					676:684	low viscosity	672:684	low viscosity	672:684	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	1	24	theme	modified	190:197	arg1	solution					250:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	3	25	theme	UW	704:705	arg1	solution					707:714	the UW solution	700:714	the UW solution	700:714	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	11	26	used	used	1634:1637	arg2	solution					1618:1625	MHL solution	1614:1625	MHL solution	1614:1625	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	11	26	used	used	1634:1637	arg2	alternative					1645:1655	an alternative	1642:1655	an alternative to MK solution for pancreatic islet transplantation	1642:1707	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	1	27	theme	extracellular-type	199:216	arg1	solution					250:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	0	28	theme	Pancreas	0:7	arg1	Preservation					9:20	Pancreas Preservation	0:20	Pancreas Preservation in Modified Histidine-lactobionate Solution	0:64	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	8	29	theme	MK	1253:1254	arg1	groups					1256:1261	the MHL and MK groups	1241:1261	groups	1256:1261	On the contrary, the islet yield before and after purification was not significantly different between the MHL and MK groups.					
36001489	9	30	theme	streptozotocin-induced	1362:1383	arg1	mice					1394:1397	streptozotocin-induced diabetic mice	1362:1397	streptozotocin-induced diabetic mice	1362:1397	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	11	31	theme	MK	1585:1586	arg1	solution					1588:1595	MK solution	1585:1595	MK solution	1585:1595	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	1	32	theme	trehalose-containing	218:237	arg1	solution					250:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	0	33	theme	Modified	25:32	arg1	Solution					57:64	Modified Histidine-lactobionate Solution	25:64	Modified Histidine-lactobionate Solution	25:64	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	11	34	theme	MHL	1614:1616	arg1	solution					1618:1625	MHL solution	1614:1625	MHL solution	1614:1625	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	11	34	theme	MHL	1614:1616	arg1	alternative					1645:1655	an alternative	1642:1655	an alternative to MK solution for pancreatic islet transplantation	1642:1707	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	0	35	theme	Islet	134:138	arg1	Isolation					140:148	Porcine Islet Isolation	126:148	Porcine Islet Isolation	126:148	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	1	36	dep	BACKGROUND	151:160	arg1	reported					176:183	reported	176:183	reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation	176:474	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	10	37	theme	Pancreas	1446:1453	arg1	preservation					1455:1466	CONCLUSIONS Pancreas preservation	1434:1466	CONCLUSIONS Pancreas preservation with MHL solution	1434:1484	CONCLUSIONS Pancreas preservation with MHL solution preserves islet function better than UW solution.					
36001489	1	38	theme	Kyoto	239:243	arg1	solution					250:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	6	39	from	group	948:952	arg1	number					902:907	An optimized number	889:907	An optimized number (1500 IE) of isolated islets from each group	889:952	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	6	39	from	group	948:952	arg1	IE					915:916	1500 IE	910:916	1500 IE	910:916	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	6	39	from	group	948:952	arg1	islets					931:936	isolated islets	922:936	isolated islets from each group	922:952	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	0	40	theme	Porcine	126:132	arg1	Isolation					140:148	Porcine Islet Isolation	126:148	Porcine Islet Isolation	126:148	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	6	41	theme	diabetic	1005:1012	arg1	mice					1014:1017	streptozotocin-induced diabetic mice	982:1017	streptozotocin-induced diabetic mice	982:1017	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	2	42	dep	UW	587:588	arg1	addition					575:582	addition	575:582	addition	575:582	In this study, we evaluated the efficiency of a modified histidine-lactobionate (MHL) solution in addition to UW or MK solution.					
36001489	11	43	theme	pancreatic	1676:1685	arg1	transplantation					1693:1707	pancreatic islet transplantation	1676:1707	pancreatic islet transplantation	1676:1707	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	2	44	theme	histidine-lactobionate	534:555	arg1	solution					563:570	a modified histidine-lactobionate (MHL) solution	523:570	a modified histidine-lactobionate (MHL) solution	523:570	In this study, we evaluated the efficiency of a modified histidine-lactobionate (MHL) solution in addition to UW or MK solution.					
36001489	3	45	theme	high	629:632	arg1	composition					655:665	a high sodium-low potassium composition	627:665	a high sodium-low potassium composition with low viscosity	627:684	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	1	46	contain	contains	266:273	arg2	ulinastatin					296:306	ulinastatin	296:306	ulinastatin	296:306	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	1	46	contain	contains	266:273	arg1	solution					250:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	modified extracellular-type trehalose-containing Kyoto (MK) solution	190:257	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	1	46	contain	contains	266:273	arg2	inhibitor					285:293	a trypsin inhibitor	275:293	a trypsin inhibitor (ulinastatin)	275:307	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	10	47	with	preservation	1455:1466	arg1	solution					1477:1484	MHL solution	1473:1484	MHL solution	1473:1484	CONCLUSIONS Pancreas preservation with MHL solution preserves islet function better than UW solution.					
36001489	6	48	theme	streptozotocin-induced	982:1003	arg1	mice					1014:1017	streptozotocin-induced diabetic mice	982:1017	streptozotocin-induced diabetic mice	982:1017	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	0	49	theme	Histidine-lactobionate	34:55	arg1	Solution					57:64	Modified Histidine-lactobionate Solution	25:64	Modified Histidine-lactobionate Solution	25:64	Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation.					
36001489	2	50	theme	modified	525:532	arg1	solution					563:570	a modified histidine-lactobionate (MHL) solution	523:570	a modified histidine-lactobionate (MHL) solution	523:570	In this study, we evaluated the efficiency of a modified histidine-lactobionate (MHL) solution in addition to UW or MK solution.					
36001489	5	51	theme	Porcine	799:805	arg1	pancreata					807:815	METHODS Porcine pancreata	791:815	METHODS Porcine pancreata	791:815	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	11	52	theme	solution	1554:1561	arg1	similar					1566:1572	similar	1566:1572	similar	1566:1572	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	11	52	theme	solution	1554:1561	arg1	effect					1540:1545	The effect	1536:1545	The effect of MHL solution	1536:1561	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	5	53	theme	UW	835:836	arg1	solution					850:857	UW, MK, or MHL solution	835:857	UW, MK, or MHL solution	835:857	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	8	54	theme	islet	1159:1163	arg1	yield					1165:1169	the islet yield	1155:1169	the islet yield before and after purification	1155:1199	On the contrary, the islet yield before and after purification was not significantly different between the MHL and MK groups.					
36001489	8	54	theme	islet	1159:1163	arg1	different					1223:1231	different	1223:1231	different	1223:1231	On the contrary, the islet yield before and after purification was not significantly different between the MHL and MK groups.					
36001489	9	55	theme	MHL	1300:1302	arg1	solution					1304:1311	MHL solution	1300:1311	MHL solution	1300:1311	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	10	56	theme	islet	1496:1500	arg1	function					1502:1509	islet function	1496:1509	islet function	1496:1509	CONCLUSIONS Pancreas preservation with MHL solution preserves islet function better than UW solution.					
36001489	10	57	theme	CONCLUSIONS	1434:1444	arg1	preservation					1455:1466	CONCLUSIONS Pancreas preservation	1434:1466	CONCLUSIONS Pancreas preservation with MHL solution	1434:1484	CONCLUSIONS Pancreas preservation with MHL solution preserves islet function better than UW solution.					
36001489	1	58	theme	islet	337:341	arg1	yield					343:347	the islet yield	333:347	the islet yield	333:347	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	11	59	theme	MK	1660:1661	arg1	solution					1663:1670	MK solution	1660:1670	MK solution	1660:1670	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	9	60	theme	UW	1421:1422	arg1	solution					1424:1431	UW solution	1421:1431	UW solution	1421:1431	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
36001489	5	61	theme	islet	872:876	arg1	isolation					878:886	islet isolation	872:886	islet isolation	872:886	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	2	62	theme	solution	563:570	arg1	efficiency					509:518	the efficiency	505:518	the efficiency of a modified histidine-lactobionate (MHL) solution	505:570	In this study, we evaluated the efficiency of a modified histidine-lactobionate (MHL) solution in addition to UW or MK solution.					
36001489	11	63	theme	MHL	1550:1552	arg1	solution					1554:1561	MHL solution	1550:1561	MHL solution	1550:1561	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	8	64	theme	MHL	1245:1247	arg1	groups					1256:1261	the MHL and MK groups	1241:1261	groups	1256:1261	On the contrary, the islet yield before and after purification was not significantly different between the MHL and MK groups.					
36001489	4	65	contain	contains	769:776	arg1	solution					755:762	MHL solution	751:762	MHL solution	751:762	Moreover, similar to MK solution, MHL solution also contains ulinastatin.					
36001489	4	65	contain	contains	769:776	arg2	ulinastatin					778:788	ulinastatin	778:788	ulinastatin	778:788	Moreover, similar to MK solution, MHL solution also contains ulinastatin.					
36001489	6	66	from	number	902:907	arg1	group					948:952	each group	943:952	each group	943:952	An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.					
36001489	1	67	theme	standard	424:431	arg1	gold					419:422	the gold	415:422	the gold standard for organ preservation for islet isolation	415:474	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	1	67	theme	standard	424:431	arg1	preservation					392:403	Wisconsin (UW) preservation	377:403	Wisconsin (UW) preservation	377:403	BACKGROUND We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation.					
36001489	3	68	theme	MHL	610:612	arg1	solution					614:621	The MHL solution	606:621	The MHL solution	606:621	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	10	69	theme	UW	1523:1524	arg1	solution					1526:1533	UW solution	1523:1533	UW solution	1523:1533	CONCLUSIONS Pancreas preservation with MHL solution preserves islet function better than UW solution.					
36001489	3	70	theme	potassium	645:653	arg1	composition					655:665	a high sodium-low potassium composition	627:665	a high sodium-low potassium composition with low viscosity	627:684	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	3	71	contain	has	623:625	arg1	solution					614:621	The MHL solution	606:621	The MHL solution	606:621	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	3	71	contain	has	623:625	arg2	composition					655:665	a high sodium-low potassium composition	627:665	a high sodium-low potassium composition with low viscosity	627:684	The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution.					
36001489	5	72	theme	MHL	846:848	arg1	solution					850:857	UW, MK, or MHL solution	835:857	UW, MK, or MHL solution	835:857	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	5	73	theme	MK	839:840	arg1	solution					850:857	UW, MK, or MHL solution	835:857	UW, MK, or MHL solution	835:857	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	7	74	dep	RESULTS	1020:1026	arg1	yield					1038:1042	The islet yield	1028:1042	The islet yield before and after purification	1028:1072	RESULTS The islet yield before and after purification was significantly higher in the MHL group than in the UW group.					
36001489	7	74	dep	RESULTS	1020:1026	arg1	higher					1092:1097	higher	1092:1097	higher	1092:1097	RESULTS The islet yield before and after purification was significantly higher in the MHL group than in the UW group.					
36001489	5	75	dep	preserved	822:830	arg1	followed					860:867	followed	860:867	followed by islet isolation	860:886	METHODS Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation.					
36001489	11	76	theme	islet	1687:1691	arg1	transplantation					1693:1707	pancreatic islet transplantation	1676:1707	pancreatic islet transplantation	1676:1707	The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.					
36001489	7	77	theme	UW	1128:1129	arg1	group					1131:1135	the UW group	1124:1135	the UW group	1124:1135	RESULTS The islet yield before and after purification was significantly higher in the MHL group than in the UW group.					
36001489	9	78	theme	islet	1337:1341	arg1	transplantation					1343:1357	islet transplantation	1337:1357	islet transplantation	1337:1357	Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.					
33086113	5	0	theme	fibrin	795:800	arg1	blend					802:806	regenerated cellulose/silk fibrin blend	768:806	regenerated cellulose/silk fibrin blend which is 120.4 mg/g	768:826	The prepared biocomposite exhibited high removal efficiency for MB (172.4 mg/g) compared to regenerated cellulose/silk fibrin blend which is 120.4 mg/g.					
33086113	1	1	theme	[Bmim	179:183	arg1	[Cl					185:187	1-Butyl-3-methylimidazolium chloride [Bmim][Cl]	142:188	1-Butyl-3-methylimidazolium chloride [Bmim][Cl]	142:188	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	1	2	theme	cellulose/silk	259:272	arg1	fibroin					274:280	regenerated cellulose/silk fibroin	247:280	regenerated cellulose/silk fibroin	247:280	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	0	3	theme	dye	81:83	arg1	adsorption					85:94	dye adsorption	81:94	dye adsorption	81:94	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	1	4	theme	fibroin	274:280	arg1	blend					238:242	homogenous blend	227:242	homogenous blend of regenerated cellulose/silk fibroin	227:280	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	4	5	theme	blue	649:652	arg1	methylene					639:647	methylene blue	639:652	methylene blue (MB)	639:657	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	4	5	theme	blue	649:652	arg1	MB					655:656	MB	655:656	MB	655:656	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	7	6	theme	phosphate	1002:1010	arg1	biocomposite					1012:1023	The regenerated cellulose/silk/calcium phosphate biocomposite	963:1023	The regenerated cellulose/silk/calcium phosphate biocomposite	963:1023	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	2	7	theme	cellulose/silk	314:327	arg1	matrix					329:334	regenerated cellulose/silk matrix	302:334	regenerated cellulose/silk matrix	302:334	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization was studied in the current article.					
33086113	2	8	theme	matrix	329:334	arg1	bioactivity					287:297	The bioactivity	283:297	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization	283:377	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization was studied in the current article.					
33086113	7	9	from	pollution	1125:1133	arg1	water					1110:1114	water	1110:1114	water from dye pollution	1110:1133	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	7	9	from	pollution	1125:1133	arg1	remediation					1095:1105	the remediation	1091:1105	the remediation of water from dye pollution	1091:1133	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	5	10	theme	prepared	680:687	arg1	biocomposite					689:700	The prepared biocomposite	676:700	The prepared biocomposite	676:700	The prepared biocomposite exhibited high removal efficiency for MB (172.4 mg/g) compared to regenerated cellulose/silk fibrin blend which is 120.4 mg/g.					
33086113	6	11	theme	kinetic	833:839	arg1	study					841:845	The kinetic study	829:845	The kinetic study	829:845	The kinetic study and the isotherm results for the examined materials followed pseudo second order and Langmuir models, respectively.					
33086113	2	12	theme	regenerated	302:312	arg1	matrix					329:334	regenerated cellulose/silk matrix	302:334	regenerated cellulose/silk matrix	302:334	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization was studied in the current article.					
33086113	6	13	theme	second	915:920	arg1	order					922:926	pseudo second order	908:926	pseudo second order	908:926	The kinetic study and the isotherm results for the examined materials followed pseudo second order and Langmuir models, respectively.					
33086113	4	14	theme	dyes	625:628	arg1	removal					606:612	removal	606:612	removal of organic dyes, such as methylene blue (MB),	606:658	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	7	15	theme	regenerated	967:977	arg1	biocomposite					1012:1023	The regenerated cellulose/silk/calcium phosphate biocomposite	963:1023	The regenerated cellulose/silk/calcium phosphate biocomposite	963:1023	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	3	16	theme	phosphate	446:454	arg1	biocomposite					456:467	Cellulose/silk fibroin/calcium phosphate biocomposite	415:467	Cellulose/silk fibroin/calcium phosphate biocomposite	415:467	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	0	17	theme	fibroin	15:21	arg1	growth					50:55	Cellulose/silk fibroin assisted calcium phosphate growth	0:55	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	6	18	theme	pseudo	908:913	arg1	order					922:926	pseudo second order	908:926	pseudo second order	908:926	The kinetic study and the isotherm results for the examined materials followed pseudo second order and Langmuir models, respectively.					
33086113	7	19	theme	cellulose/silk/calcium	979:1000	arg1	biocomposite					1012:1023	The regenerated cellulose/silk/calcium phosphate biocomposite	963:1023	The regenerated cellulose/silk/calcium phosphate biocomposite	963:1023	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	2	20	theme	calcium	346:352	arg1	mineralization					364:377	calcium phosphate mineralization	346:377	calcium phosphate mineralization	346:377	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization was studied in the current article.					
33086113	3	21	theme	different	489:497	arg1	EDX					557:559	EDX	557:559	EDX	557:559	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	21	theme	different	489:497	arg1	methods					516:522	different characterization methods	489:522	different characterization methods such as FT-IR, XRD, TGA, SEM and EDX	489:559	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	21	theme	different	489:497	arg1	TGA					544:546	TGA	544:546	TGA	544:546	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	21	theme	different	489:497	arg1	SEM					549:551	SEM	549:551	SEM	549:551	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	21	theme	different	489:497	arg1	FT-IR					532:536	FT-IR	532:536	FT-IR	532:536	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	21	theme	different	489:497	arg1	XRD					539:541	XRD	539:541	XRD	539:541	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	0	22	theme	Cellulose/silk	0:13	arg1	growth					50:55	Cellulose/silk fibroin assisted calcium phosphate growth	0:55	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	3	23	theme	characterization	499:514	arg1	EDX					557:559	EDX	557:559	EDX	557:559	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	23	theme	characterization	499:514	arg1	methods					516:522	different characterization methods	489:522	different characterization methods such as FT-IR, XRD, TGA, SEM and EDX	489:559	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	23	theme	characterization	499:514	arg1	TGA					544:546	TGA	544:546	TGA	544:546	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	23	theme	characterization	499:514	arg1	SEM					549:551	SEM	549:551	SEM	549:551	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	23	theme	characterization	499:514	arg1	FT-IR					532:536	FT-IR	532:536	FT-IR	532:536	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	3	23	theme	characterization	499:514	arg1	XRD					539:541	XRD	539:541	XRD	539:541	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	0	24	theme	calcium	32:38	arg1	growth					50:55	Cellulose/silk fibroin assisted calcium phosphate growth	0:55	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	4	25	theme	composite	592:600	arg1	potential					566:574	The potential	562:574	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB),	562:658	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	0	26	theme	assisted	23:30	arg1	growth					50:55	Cellulose/silk fibroin assisted calcium phosphate growth	0:55	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	1	27	theme	silk	111:114	arg1	fibroin					116:122	silk fibroin	111:122	silk fibroin	111:122	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	0	28	theme	phosphate	40:48	arg1	growth					50:55	Cellulose/silk fibroin assisted calcium phosphate growth	0:55	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	4	29	theme	organic	617:623	arg1	methylene					639:647	methylene blue	639:652	methylene blue (MB)	639:657	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	4	29	theme	organic	617:623	arg1	dyes					625:628	organic dyes	617:628	organic dyes	617:628	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	6	30	theme	examined	880:887	arg1	materials					889:897	the examined materials	876:897	the examined materials	876:897	The kinetic study and the isotherm results for the examined materials followed pseudo second order and Langmuir models, respectively.					
33086113	0	31	theme	Novel	58:62	arg1	biocomposite					64:75	Novel biocomposite	58:75	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	0	32	dep	growth	50:55	arg1	biocomposite					64:75	Novel biocomposite	58:75	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.	0:95	Cellulose/silk fibroin assisted calcium phosphate growth: Novel biocomposite for dye adsorption.					
33086113	7	33	theme	dye	1121:1123	arg1	pollution					1125:1133	dye pollution	1121:1133	dye pollution	1121:1133	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	6	34	theme	Langmuir	932:939	arg1	models					941:946	Langmuir models	932:946	Langmuir models	932:946	The kinetic study and the isotherm results for the examined materials followed pseudo second order and Langmuir models, respectively.					
33086113	4	35	theme	prepared	583:590	arg1	composite					592:600	the prepared composite	579:600	the prepared composite	579:600	The potential of the prepared composite for removal of organic dyes, such as methylene blue (MB), was calculated.					
33086113	7	36	theme	water	1110:1114	arg1	remediation					1095:1105	the remediation	1091:1105	the remediation of water from dye pollution	1091:1133	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	1	37	theme	chloride	170:177	arg1	[Cl					185:187	1-Butyl-3-methylimidazolium chloride [Bmim][Cl]	142:188	1-Butyl-3-methylimidazolium chloride [Bmim][Cl]	142:188	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	5	38	theme	high	712:715	arg1	efficiency					725:734	high removal efficiency	712:734	high removal efficiency for MB (172.4 mg/g)	712:754	The prepared biocomposite exhibited high removal efficiency for MB (172.4 mg/g) compared to regenerated cellulose/silk fibrin blend which is 120.4 mg/g.					
33086113	1	39	theme	homogenous	227:236	arg1	blend					238:242	homogenous blend	227:242	homogenous blend of regenerated cellulose/silk fibroin	227:280	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	5	40	theme	removal	717:723	arg1	efficiency					725:734	high removal efficiency	712:734	high removal efficiency for MB (172.4 mg/g)	712:754	The prepared biocomposite exhibited high removal efficiency for MB (172.4 mg/g) compared to regenerated cellulose/silk fibrin blend which is 120.4 mg/g.					
33086113	6	41	theme	isotherm	855:862	arg1	results					864:870	the isotherm results	851:870	the isotherm results	851:870	The kinetic study and the isotherm results for the examined materials followed pseudo second order and Langmuir models, respectively.					
33086113	2	42	theme	phosphate	354:362	arg1	mineralization					364:377	calcium phosphate mineralization	346:377	calcium phosphate mineralization	346:377	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization was studied in the current article.					
33086113	7	43	from	remediation	1095:1105	arg1	pollution					1125:1133	dye pollution	1121:1133	dye pollution	1121:1133	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	5	44	theme	regenerated	768:778	arg1	blend					802:806	regenerated cellulose/silk fibrin blend	768:806	regenerated cellulose/silk fibrin blend which is 120.4 mg/g	768:826	The prepared biocomposite exhibited high removal efficiency for MB (172.4 mg/g) compared to regenerated cellulose/silk fibrin blend which is 120.4 mg/g.					
33086113	7	45	theme	further	1055:1061	arg1	research					1063:1070	further research	1055:1070	further research	1055:1070	The regenerated cellulose/silk/calcium phosphate biocomposite, thus providing prospects for further research and application in the remediation of water from dye pollution.					
33086113	1	46	theme	1-Butyl-3-methylimidazolium	142:168	arg1	[Cl					185:187	1-Butyl-3-methylimidazolium chloride [Bmim][Cl]	142:188	1-Butyl-3-methylimidazolium chloride [Bmim][Cl]	142:188	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
33086113	3	47	theme	fibroin/calcium	430:444	arg1	biocomposite					456:467	Cellulose/silk fibroin/calcium phosphate biocomposite	415:467	Cellulose/silk fibroin/calcium phosphate biocomposite	415:467	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	5	48	theme	cellulose/silk	780:793	arg1	blend					802:806	regenerated cellulose/silk fibrin blend	768:806	regenerated cellulose/silk fibrin blend which is 120.4 mg/g	768:826	The prepared biocomposite exhibited high removal efficiency for MB (172.4 mg/g) compared to regenerated cellulose/silk fibrin blend which is 120.4 mg/g.					
33086113	3	49	theme	Cellulose/silk	415:428	arg1	biocomposite					456:467	Cellulose/silk fibroin/calcium phosphate biocomposite	415:467	Cellulose/silk fibroin/calcium phosphate biocomposite	415:467	Cellulose/silk fibroin/calcium phosphate biocomposite was investigated by different characterization methods such as FT-IR, XRD, TGA, SEM and EDX.					
33086113	2	50	theme	current	398:404	arg1	article					406:412	the current article	394:412	the current article	394:412	The bioactivity of regenerated cellulose/silk matrix to assist calcium phosphate mineralization was studied in the current article.					
33086113	1	51	theme	regenerated	247:257	arg1	fibroin					274:280	regenerated cellulose/silk fibroin	247:280	regenerated cellulose/silk fibroin	247:280	Cellulose and silk fibroin were dissolved in 1-Butyl-3-methylimidazolium chloride [Bmim][Cl] and regenerated with ethanol to form homogenous blend of regenerated cellulose/silk fibroin.					
32526333	0	0	theme	drug	95:98	arg1	tablets					100:106	low dose drug tablets	86:106	low dose drug tablets	86:106	A microcrystalline cellulose based drug-composite formulation strategy for developing low dose drug tablets.					
32526333	4	1	theme	BEAM	553:556	arg1	powders					558:564	BEAM powders	553:564	BEAM powders of 5 model APIs	553:580	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	0	2	theme	dose	90:93	arg1	tablets					100:106	low dose drug tablets	86:106	low dose drug tablets	86:106	A microcrystalline cellulose based drug-composite formulation strategy for developing low dose drug tablets.					
32526333	3	3	theme	powder	488:493	arg1	bed					495:497	a powder bed	486:497	a powder bed of microcrystalline cellulose (MCC) under high shear	486:550	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	6	4	theme	formulation	952:962	arg1	strategy					964:971	this formulation strategy	947:971	this formulation strategy	947:971	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	3	5	theme	BEAM	350:353	arg1	approach					355:362	the BEAM approach	346:362	the BEAM approach	346:362	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	4	6	theme	ejection	702:709	arg1	force					711:715	low ejection force	698:715	low ejection force	698:715	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	2	7	theme	cellulose	290:298	arg1	approach					307:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach	251:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge	251:340	Here, we present a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge.					
32526333	5	8	theme	other	860:864	arg1	excipients					866:875	any other excipients	856:875	any other excipients	856:875	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	6	9	theme	platform	1028:1035	arg1	technology					1037:1046	a potential platform technology	1016:1046	a potential platform technology for manufacturing tablets of potent APIs	1016:1087	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	3	10	theme	microcrystalline	502:517	arg1	MCC					530:532	MCC	530:532	MCC	530:532	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	3	10	theme	microcrystalline	502:517	arg1	cellulose					519:527	microcrystalline cellulose	502:527	microcrystalline cellulose (MCC)	502:533	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	4	11	theme	orders	621:626	arg1	range					610:614	a range	608:614	a range of 5 orders of magnitude	608:639	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	5	12	theme	API	803:805	arg1	uniformity					807:816	excellent API uniformity	793:816	excellent API uniformity	793:816	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	6	13	theme	potent	1077:1082	arg1	APIs					1084:1087	potent APIs	1077:1087	potent APIs	1077:1087	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	2	14	theme	enhanced	260:267	arg1	approach					307:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach	251:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge	251:340	Here, we present a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge.					
32526333	1	15	theme	main	171:174	arg1	challenge					176:184	a main challenge	169:184	a main challenge associated with manufacturing low dose tablets	169:231	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	1	15	theme	main	171:174	arg1	uniformity					113:122	The uniformity	109:122	The uniformity of active pharmaceutical ingredient (API)	109:164	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	0	16	theme	microcrystalline	2:17	arg1	cellulose					19:27	A microcrystalline cellulose	0:27	A microcrystalline cellulose	0:27	A microcrystalline cellulose based drug-composite formulation strategy for developing low dose drug tablets.					
32526333	3	17	theme	high	541:544	arg1	shear					546:550	high shear	541:550	high shear	541:550	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	3	18	theme	hydro-alcoholic	403:417	arg1	solution					419:426	a PVP hydro-alcoholic solution	397:426	a PVP hydro-alcoholic solution	397:426	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	5	19	theme	fast	822:825	arg1	disintegration					827:840	fast disintegration	822:840	fast disintegration	822:840	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	6	20	theme	uniformity	926:935	arg1	problems					937:944	content uniformity problems	918:944	content uniformity problems	918:944	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	4	21	theme	low	698:700	arg1	force					711:715	low ejection force	698:715	low ejection force	698:715	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	6	22	theme	content	918:924	arg1	problems					937:944	content uniformity problems	918:944	content uniformity problems	918:944	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	2	23	theme	API-microcrystalline	269:288	arg1	approach					307:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach	251:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge	251:340	Here, we present a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge.					
32526333	2	24	theme	BEAM	301:304	arg1	approach					307:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach	251:314	a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge	251:340	Here, we present a binder enhanced API-microcrystalline cellulose (BEAM) approach to address this challenge.					
32526333	1	25	theme	manufacturing	202:214	arg1	tablets					225:231	manufacturing low dose tablets	202:231	manufacturing low dose tablets	202:231	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	0	26	theme	formulation	50:60	arg1	strategy					62:69	drug-composite formulation strategy	35:69	drug-composite formulation strategy for developing low dose drug tablets	35:106	A microcrystalline cellulose based drug-composite formulation strategy for developing low dose drug tablets.					
32526333	6	27	theme	APIs	1084:1087	arg1	tablets					1066:1072	tablets	1066:1072	tablets of potent APIs	1066:1087	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	3	28	contain	contains	435:442	arg2	API					444:446	API	444:446	API	444:446	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	3	28	contain	contains	435:442	arg1	solution					419:426	a PVP hydro-alcoholic solution	397:426	a PVP hydro-alcoholic solution	397:426	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	3	28	contain	contains	435:442	arg2	solution					419:426	a PVP hydro-alcoholic solution	397:426	a PVP hydro-alcoholic solution	397:426	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	3	28	contain	contains	435:442	arg1	concentration					466:478	an appropriate concentration	451:478	an appropriate concentration	451:478	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	1	29	theme	low	216:218	arg1	tablets					225:231	manufacturing low dose tablets	202:231	manufacturing low dose tablets	202:231	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	0	30	theme	drug-composite	35:48	arg1	strategy					62:69	drug-composite formulation strategy	35:69	drug-composite formulation strategy for developing low dose drug tablets	35:106	A microcrystalline cellulose based drug-composite formulation strategy for developing low dose drug tablets.					
32526333	3	31	theme	PVP	399:401	arg1	solution					419:426	a PVP hydro-alcoholic solution	397:426	a PVP hydro-alcoholic solution	397:426	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	1	32	theme	dose	220:223	arg1	tablets					225:231	manufacturing low dose tablets	202:231	manufacturing low dose tablets	202:231	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	5	33	theme	BEAM	733:736	arg1	powders					738:744	all BEAM powders	729:744	all BEAM powders	729:744	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	6	34	dep	ways	902:905	arg1	address					910:916	address	910:916	to address content uniformity problems	907:944	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	4	35	theme	excellent	656:664	arg1	flowability					666:676	excellent flowability	656:676	excellent flowability	656:676	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	5	36	with	tablets	780:786	arg1	uniformity					807:816	excellent API uniformity	793:816	excellent API uniformity	793:816	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	5	36	with	tablets	780:786	arg1	disintegration					827:840	fast disintegration	822:840	fast disintegration	822:840	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	6	37	theme	traditional	890:900	arg1	ways					902:905	traditional ways	890:905	traditional ways to address content uniformity problems	890:944	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	1	38	theme	active	127:132	arg1	API					161:163	API	161:163	API	161:163	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	1	38	theme	active	127:132	arg1	ingredient					149:158	active pharmaceutical ingredient	127:158	active pharmaceutical ingredient (API)	127:164	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	1	39	theme	pharmaceutical	134:147	arg1	API					161:163	API	161:163	API	161:163	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	1	39	theme	pharmaceutical	134:147	arg1	ingredient					149:158	active pharmaceutical ingredient	127:158	active pharmaceutical ingredient (API)	127:164	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	4	40	theme	model	571:575	arg1	APIs					577:580	5 model APIs	569:580	5 model APIs	569:580	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	3	41	theme	cellulose	519:527	arg1	bed					495:497	a powder bed	486:497	a powder bed of microcrystalline cellulose (MCC) under high shear	486:550	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	1	42	theme	ingredient	149:158	arg1	challenge					176:184	a main challenge	169:184	a main challenge associated with manufacturing low dose tablets	169:231	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	1	42	theme	ingredient	149:158	arg1	uniformity					113:122	The uniformity	109:122	The uniformity of active pharmaceutical ingredient (API)	109:164	The uniformity of active pharmaceutical ingredient (API) is a main challenge associated with manufacturing low dose tablets.					
32526333	3	43	theme	appropriate	454:464	arg1	concentration					466:478	an appropriate concentration	451:478	an appropriate concentration	451:478	In the BEAM approach a powder is prepared by spraying a PVP hydro-alcoholic solution, which contains API at an appropriate concentration, onto a powder bed of microcrystalline cellulose (MCC) under high shear.					
32526333	0	44	theme	low	86:88	arg1	tablets					100:106	low dose drug tablets	86:106	low dose drug tablets	86:106	A microcrystalline cellulose based drug-composite formulation strategy for developing low dose drug tablets.					
32526333	5	45	theme	excellent	793:801	arg1	uniformity					807:816	excellent API uniformity	793:816	excellent API uniformity	793:816	Therefore, all BEAM powders could be directly compressed into tablets with excellent API uniformity and fast disintegration without using any other excipients.					
32526333	4	46	theme	magnitude	631:639	arg1	orders					621:626	5 orders	619:626	5 orders of magnitude	619:639	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
32526333	6	47	theme	potential	1018:1026	arg1	technology					1037:1046	a potential platform technology	1016:1046	a potential platform technology for manufacturing tablets of potent APIs	1016:1087	Compared to traditional ways to address content uniformity problems, this formulation strategy is much more robust and simpler, making it a potential platform technology for manufacturing tablets of potent APIs.					
32526333	4	48	theme	APIs	577:580	arg1	powders					558:564	BEAM powders	553:564	BEAM powders of 5 model APIs	553:580	BEAM powders of 5 model APIs, with solubility spanning a range of 5 orders of magnitude, all exhibited excellent flowability, tabletability, and low ejection force.					
34592227	5	0	theme	high	799:802	arg1	absorbency					810:819	high water absorbency	799:819	high water absorbency	799:819	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	0	theme	high	799:802	arg1	structure					760:768	microporous structure	748:768	microporous structure	748:768	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	6	1	theme	drug	937:940	arg1	properties					950:959	the sustained drug release properties	923:959	the sustained drug release properties in vitro	923:968	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	5	2	dep	vapor	839:843	arg1	rate					858:861	transmission rate	845:861	microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate	748:861	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	6	3	theme	composite	891:899	arg1	dressing					907:914	the composite wound dressing	887:914	the composite wound dressing	887:914	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	1	4	theme	drug-resistant	183:196	arg1	bacteria					198:205	massive drug-resistant bacteria	175:205	massive drug-resistant bacteria	175:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	0	5	theme	dressing	98:105	arg1	Preparation					0:10	Preparation	0:10	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.	0:134	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	6	6	theme	sustained	927:935	arg1	properties					950:959	the sustained drug release properties	923:959	the sustained drug release properties in vitro	923:968	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	7	7	theme	good	1152:1155	arg1	biocompatibility					1157:1172	good biocompatibility	1152:1172	good biocompatibility	1152:1172	The cytotoxicity results demonstrated that the composite hydrogels had good biocompatibility.					
34592227	1	8	theme	bacteria	198:205	arg1	abuse					148:152	abuse	148:152	abuse	148:152	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	1	8	theme	bacteria	198:205	arg1	emergence					162:170	the emergence	158:170	the emergence of massive drug-resistant bacteria	158:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	1	8	theme	bacteria	198:205	arg1	Antibiotics					136:146	Antibiotics abuse and the emergence of massive drug-resistant bacteria	136:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria	136:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	6	9	dep	aureus	1030:1035	arg1	aureus					1041:1046	S. aureus	1038:1046	S. aureus	1038:1046	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	3	10	theme	sandwich-like	401:413	arg1	dressings					440:448	the sandwich-like composite hydrogel wound dressings	397:448	the sandwich-like composite hydrogel wound dressings	397:448	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	10	theme	sandwich-like	401:413	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	7	11	theme	composite	1128:1136	arg1	hydrogels					1138:1146	the composite hydrogels	1124:1146	the composite hydrogels	1124:1146	The cytotoxicity results demonstrated that the composite hydrogels had good biocompatibility.					
34592227	3	12	theme	Centella	576:583	arg1	CA					595:596	CA	595:596	CA	595:596	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	12	theme	Centella	576:583	arg1	asiatica					585:592	Centella asiatica	576:592	Centella asiatica (CA) as the base materials	576:619	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	7	13	contain	had	1148:1150	arg1	hydrogels					1138:1146	the composite hydrogels	1124:1146	the composite hydrogels	1124:1146	The cytotoxicity results demonstrated that the composite hydrogels had good biocompatibility.					
34592227	7	13	contain	had	1148:1150	arg2	biocompatibility					1157:1172	good biocompatibility	1152:1172	good biocompatibility	1152:1172	The cytotoxicity results demonstrated that the composite hydrogels had good biocompatibility.					
34592227	0	14	theme	antibacterial	112:124	arg1	property					126:133	antibacterial property	112:133	antibacterial property	112:133	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	6	15	dep	coli	1065:1068	arg1	coli					1074:1077	E. coli	1071:1077	E. coli	1071:1077	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	2	16	theme	antibacterial	317:329	arg1	activity					331:338	antibacterial activity	317:338	antibacterial activity	317:338	Thus, designing an antibiotic-free wound dressing with antibacterial activity and decent biocompatibility is urgently desired.					
34592227	3	17	theme	gelatin	525:531	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	17	theme	gelatin	525:531	arg1	hydrogel					555:562	gelatin and chitosan (Gel-CS) hydrogel	525:562	gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials	525:619	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	5	18	theme	stable	771:776	arg1	structure					760:768	microporous structure	748:768	microporous structure	748:768	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	18	theme	stable	771:776	arg1	property					789:796	stable mechanical property	771:796	stable mechanical property	771:796	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	19	theme	mechanical	778:787	arg1	structure					760:768	microporous structure	748:768	microporous structure	748:768	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	19	theme	mechanical	778:787	arg1	property					789:796	stable mechanical property	771:796	stable mechanical property	771:796	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	6	20	theme	wound	901:905	arg1	dressing					907:914	the composite wound dressing	887:914	the composite wound dressing	887:914	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	3	21	theme	intercalating	468:480	arg1	NF					500:501	NF	500:501	NF	500:501	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	21	theme	intercalating	468:480	arg1	fabrics					491:497	intercalating nonwoven fabrics	468:497	intercalating nonwoven fabrics (NF)	468:502	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	8	22	theme	composite	1213:1221	arg1	hydrogels					1223:1231	the nonwoven composite hydrogels	1200:1231	the nonwoven composite hydrogels	1200:1231	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	5	23	theme	water	804:808	arg1	absorbency					810:819	high water absorbency	799:819	high water absorbency	799:819	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	23	theme	water	804:808	arg1	structure					760:768	microporous structure	748:768	microporous structure	748:768	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	8	24	theme	medical	1282:1288	arg1	care					1290:1293	medical care	1282:1293	medical care in the future	1282:1307	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	8	25	theme	care	1290:1293	arg1	field					1273:1277	the field	1269:1277	the field of medical care in the future	1269:1307	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	5	26	theme	water	833:837	arg1	vapor					839:843	water vapor	833:843	microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate	748:861	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	8	27	from	care	1290:1293	arg1	future					1302:1307	future	1302:1307	future	1302:1307	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	1	28	theme	major	223:227	arg1	obstacles					229:237	the major obstacles	219:237	the major obstacles in the medical system	219:259	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	0	29	theme	asiatica	24:31	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	8	30	theme	broad	1238:1242	arg1	prospects					1256:1264	broad application prospects	1238:1264	broad application prospects	1238:1264	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	2	31	theme	decent	344:349	arg1	biocompatibility					351:366	decent biocompatibility	344:366	decent biocompatibility	344:366	Thus, designing an antibiotic-free wound dressing with antibacterial activity and decent biocompatibility is urgently desired.					
34592227	3	32	theme	middle	511:516	arg1	dressings					440:448	the sandwich-like composite hydrogel wound dressings	397:448	the sandwich-like composite hydrogel wound dressings	397:448	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	32	theme	middle	511:516	arg1	hydrogel					555:562	gelatin and chitosan (Gel-CS) hydrogel	525:562	gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials	525:619	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	32	theme	middle	511:516	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	0	33	theme	Centella	15:22	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	6	34	theme	antibacterial	984:996	arg1	activity					998:1005	excellent antibacterial activity	974:1005	excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)	974:1078	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	7	35	theme	cytotoxicity	1085:1096	arg1	results					1098:1104	The cytotoxicity results	1081:1104	The cytotoxicity results	1081:1104	The cytotoxicity results demonstrated that the composite hydrogels had good biocompatibility.					
34592227	0	36	theme	gelatin/chitosan/nonwoven	40:64	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	6	37	theme	excellent	974:982	arg1	activity					998:1005	excellent antibacterial activity	974:1005	excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)	974:1078	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	5	38	theme	microporous	748:758	arg1	structure					760:768	microporous structure	748:768	microporous structure	748:768	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	38	theme	microporous	748:758	arg1	absorbency					810:819	high water absorbency	799:819	high water absorbency	799:819	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	5	38	theme	microporous	748:758	arg1	property					789:796	stable mechanical property	771:796	stable mechanical property	771:796	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	4	39	theme	mechanical	680:689	arg1	properties					691:700	the mechanical properties	676:700	the mechanical properties of hydrogels	676:713	In addition, soaking strategy was employed to improve the mechanical properties of hydrogels.					
34592227	6	40	with	loading	870:876	arg1	CA					883:884	CA	883:884	CA	883:884	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	0	41	theme	loaded	33:38	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	3	42	theme	nonwoven	482:489	arg1	NF					500:501	NF	500:501	NF	500:501	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	42	theme	nonwoven	482:489	arg1	fabrics					491:497	intercalating nonwoven fabrics	468:497	intercalating nonwoven fabrics (NF)	468:502	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	43	theme	hydrogel	425:432	arg1	dressings					440:448	the sandwich-like composite hydrogel wound dressings	397:448	the sandwich-like composite hydrogel wound dressings	397:448	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	43	theme	hydrogel	425:432	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	6	44	from	activity	998:1005	arg1	vitro					964:968	vitro	964:968	vitro	964:968	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	1	45	theme	medical	246:252	arg1	system					254:259	the medical system	242:259	the medical system	242:259	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	0	46	theme	composite	73:81	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	5	47	theme	transmission	845:856	arg1	rate					858:861	transmission rate	845:861	microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate	748:861	The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate.					
34592227	0	48	theme	fabric	66:71	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	6	49	from	properties	950:959	arg1	vitro					964:968	vitro	964:968	vitro	964:968	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	3	50	theme	base	606:609	arg1	materials					611:619	the base materials	602:619	the base materials	602:619	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	1	51	from	obstacles	229:237	arg1	system					254:259	the medical system	242:259	the medical system	242:259	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	3	52	theme	chitosan	537:544	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	52	theme	chitosan	537:544	arg1	hydrogel					555:562	gelatin and chitosan (Gel-CS) hydrogel	525:562	gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials	525:619	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	8	53	contain	have	1233:1236	arg2	prospects					1256:1264	broad application prospects	1238:1264	broad application prospects	1238:1264	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	8	53	contain	have	1233:1236	arg1	hydrogels					1223:1231	the nonwoven composite hydrogels	1200:1231	the nonwoven composite hydrogels	1200:1231	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	2	54	theme	wound	297:301	arg1	dressing					303:310	an antibiotic-free wound dressing	278:310	an antibiotic-free wound dressing	278:310	Thus, designing an antibiotic-free wound dressing with antibacterial activity and decent biocompatibility is urgently desired.					
34592227	0	55	with	Preparation	0:10	arg1	property					126:133	antibacterial property	112:133	antibacterial property	112:133	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	8	56	from	field	1273:1277	arg1	future					1302:1307	future	1302:1307	future	1302:1307	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	2	57	theme	antibiotic-free	281:295	arg1	dressing					303:310	an antibiotic-free wound dressing	278:310	an antibiotic-free wound dressing	278:310	Thus, designing an antibiotic-free wound dressing with antibacterial activity and decent biocompatibility is urgently desired.					
34592227	1	58	theme	massive	175:181	arg1	bacteria					198:205	massive drug-resistant bacteria	175:205	massive drug-resistant bacteria	175:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	8	59	theme	application	1244:1254	arg1	prospects					1256:1264	broad application prospects	1238:1264	broad application prospects	1238:1264	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	0	60	theme	wound	92:96	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	8	61	from	future	1302:1307	arg1	field					1273:1277	the field	1269:1277	the field of medical care in the future	1269:1307	This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.					
34592227	4	62	theme	hydrogels	705:713	arg1	properties					691:700	the mechanical properties	676:700	the mechanical properties of hydrogels	676:713	In addition, soaking strategy was employed to improve the mechanical properties of hydrogels.					
34592227	4	63	theme	soaking	635:641	arg1	strategy					643:650	soaking strategy	635:650	soaking strategy	635:650	In addition, soaking strategy was employed to improve the mechanical properties of hydrogels.					
34592227	0	64	theme	hydrogel	83:90	arg1	dressing					98:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing	15:105	Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property.					
34592227	6	65	theme	release	942:948	arg1	properties					950:959	the sustained drug release properties	923:959	the sustained drug release properties in vitro	923:968	After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli).					
34592227	3	66	theme	wound	434:438	arg1	dressings					440:448	the sandwich-like composite hydrogel wound dressings	397:448	the sandwich-like composite hydrogel wound dressings	397:448	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	66	theme	wound	434:438	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	1	67	dep	Antibiotics	136:146	arg1	abuse					148:152	abuse	148:152	abuse	148:152	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	1	67	dep	Antibiotics	136:146	arg1	emergence					162:170	the emergence	158:170	the emergence of massive drug-resistant bacteria	158:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	1	67	dep	Antibiotics	136:146	arg1	Antibiotics					136:146	Antibiotics abuse and the emergence of massive drug-resistant bacteria	136:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria	136:205	Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system.					
34592227	3	68	theme	composite	415:423	arg1	dressings					440:448	the sandwich-like composite hydrogel wound dressings	397:448	the sandwich-like composite hydrogel wound dressings	397:448	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34592227	3	68	theme	composite	415:423	arg1	layer					518:522	the middle layer	507:522	the middle layer	507:522	Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials.					
34364572	6	0	theme	Ti	923:924	arg1	plate					926:930	the MHAP/Starch/WST composite coated Ti plate	886:930	the MHAP/Starch/WST composite coated Ti plate	886:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	7	1	theme	regeneration	1123:1134	arg1	application					1136:1146	bone regeneration application	1118:1146	bone regeneration application	1118:1146	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	7	2	theme	cells	985:989	arg1	viability					991:999	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	1	3	theme	bio-medical	184:194	arg1	applications					196:207	bio-medical applications	184:207	bio-medical applications due to their numerous uses	184:234	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	7	4	theme	MHAP/Starch/WST	1044:1058	arg1	composite					1060:1068	MHAP/Starch/WST composite	1044:1068	MHAP/Starch/WST composite	1044:1068	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	4	5	theme	Ti	616:617	arg1	plate					619:623	the orthopedic grade Ti plate	595:623	the orthopedic grade Ti plate	595:623	It was successfully coated on the orthopedic grade Ti plate by the Electrophoretic Deposition (EPD) method.					
34364572	0	6	theme	bone	100:103	arg1	formation					105:113	new bone formation	96:113	new bone formation	96:113	Fabrication of substituted hydroxyapatite-starch-clay bio-composite coated titanium implant for new bone formation.					
34364572	6	7	theme	coated	916:921	arg1	plate					926:930	the MHAP/Starch/WST composite coated Ti plate	886:930	the MHAP/Starch/WST composite coated Ti plate	886:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	7	8	theme	in-vitro	959:966	arg1	cells					985:989	The in-vitro MG-63 osteoblast cells	955:989	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	3	9	theme	in-situ	532:538	arg1	method					557:562	in-situ co-precipitation method	532:562	in-situ co-precipitation method	532:562	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	4	10	theme	grade	610:614	arg1	plate					619:623	the orthopedic grade Ti plate	595:623	the orthopedic grade Ti plate	595:623	It was successfully coated on the orthopedic grade Ti plate by the Electrophoretic Deposition (EPD) method.					
34364572	5	11	theme	composite	744:752	arg1	functionality					677:689	The functionality	673:689	The functionality	673:689	The functionality, phase, morphology, and bio-activity analysis of the composite were evaluated by FT-IR, XRD, HR-TEM, and SEM analysis, respectively.					
34364572	5	11	theme	composite	744:752	arg1	phase					692:696	phase	692:696	phase	692:696	The functionality, phase, morphology, and bio-activity analysis of the composite were evaluated by FT-IR, XRD, HR-TEM, and SEM analysis, respectively.					
34364572	5	11	theme	composite	744:752	arg1	morphology					699:708	morphology	699:708	morphology	699:708	The functionality, phase, morphology, and bio-activity analysis of the composite were evaluated by FT-IR, XRD, HR-TEM, and SEM analysis, respectively.					
34364572	5	11	theme	composite	744:752	arg1	analysis					728:735	bio-activity analysis	715:735	bio-activity analysis	715:735	The functionality, phase, morphology, and bio-activity analysis of the composite were evaluated by FT-IR, XRD, HR-TEM, and SEM analysis, respectively.					
34364572	1	12	contain	have	144:147	arg2	attention					154:162	much attention	149:162	much attention from researchers	149:179	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	1	12	contain	have	144:147	arg1	matrices					135:142	The clay/polymeric matrices	116:142	The clay/polymeric matrices	116:142	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	7	13	theme	MG-63	968:972	arg1	cells					985:989	The in-vitro MG-63 osteoblast cells	955:989	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	6	14	theme	MHAP/Starch/WST	890:904	arg1	plate					926:930	the MHAP/Starch/WST composite coated Ti plate	886:930	the MHAP/Starch/WST composite coated Ti plate	886:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	7	15	theme	bone	1118:1121	arg1	application					1136:1146	bone regeneration application	1118:1146	bone regeneration application	1118:1146	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	1	16	from	researchers	169:179	arg1	attention					154:162	much attention	149:162	much attention from researchers	149:179	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	2	17	theme	composite	387:395	arg1	coating					397:403	bio-compatible composite coating	372:403	bio-compatible composite coating	372:403	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	7	18	used	used	1109:1112	arg2	implant					1093:1099	this new implant	1084:1099	this new implant	1084:1099	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	1	19	theme	due	209:211	arg1	applications					196:207	bio-medical applications	184:207	bio-medical applications due to their numerous uses	184:234	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	3	20	theme	combined	430:437	arg1	minerals					439:446	Wollastonite (WST) clay combined minerals	406:446	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	6	21	dep	value	877:881	arg1	i.e.					849:852	i.e.	849:852	i.e.	849:852	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	3	22	theme	substituted	463:473	arg1	composite					503:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	7	23	theme	clinical	1188:1195	arg1	studies					1197:1203	clinical studies	1188:1203	clinical studies	1188:1203	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	3	24	dep	minerals	439:446	arg1	Mg2+and					449:455	Mg2+and	449:455	Mg2+and	449:455	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	7	25	theme	studies	1197:1203	arg1	evaluation					1162:1171	careful evaluation	1154:1171	careful evaluation of in-vivo and clinical studies	1154:1203	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	0	26	theme	hydroxyapatite-starch-clay	27:52	arg1	bio-composite					54:66	substituted hydroxyapatite-starch-clay bio-composite	15:66	substituted hydroxyapatite-starch-clay bio-composite	15:66	Fabrication of substituted hydroxyapatite-starch-clay bio-composite coated titanium implant for new bone formation.					
34364572	5	27	theme	bio-activity	715:726	arg1	analysis					728:735	bio-activity analysis	715:735	bio-activity analysis	715:735	The functionality, phase, morphology, and bio-activity analysis of the composite were evaluated by FT-IR, XRD, HR-TEM, and SEM analysis, respectively.					
34364572	1	28	theme	clay/polymeric	120:133	arg1	matrices					135:142	The clay/polymeric matrices	116:142	The clay/polymeric matrices	116:142	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	3	29	theme	HAP	491:493	arg1	composite					503:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	0	30	theme	substituted	15:25	arg1	bio-composite					54:66	substituted hydroxyapatite-starch-clay bio-composite	15:66	substituted hydroxyapatite-starch-clay bio-composite	15:66	Fabrication of substituted hydroxyapatite-starch-clay bio-composite coated titanium implant for new bone formation.					
34364572	4	31	theme	orthopedic	599:608	arg1	plate					619:623	the orthopedic grade Ti plate	595:623	the orthopedic grade Ti plate	595:623	It was successfully coated on the orthopedic grade Ti plate by the Electrophoretic Deposition (EPD) method.					
34364572	7	32	theme	new	1089:1091	arg1	implant					1093:1099	this new implant	1084:1099	this new implant	1084:1099	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	6	33	theme	microhardness	863:875	arg1	value					877:881	Vickers microhardness value	855:881	Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate	855:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	2	34	theme	bio-compatible	372:385	arg1	coating					397:403	bio-compatible composite coating	372:403	bio-compatible composite coating	372:403	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	4	35	theme	EPD	660:662	arg1	method					665:670	the Electrophoretic Deposition (EPD) method	628:670	the Electrophoretic Deposition (EPD) method	628:670	It was successfully coated on the orthopedic grade Ti plate by the Electrophoretic Deposition (EPD) method.					
34364572	3	36	theme	minerals	439:446	arg1	composite					503:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	2	37	theme	implant	359:365	arg1	surface					341:347	the surface	337:347	the surface of the Ti implant	337:365	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	6	38	theme	Vickers	855:861	arg1	value					877:881	Vickers microhardness value	855:881	Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate	855:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	2	39	theme	titanium	274:281	arg1	implant					288:294	new orthopedic titanium (Ti) implant	259:294	new orthopedic titanium (Ti) implant	259:294	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	3	40	theme	hydroxyapatite	475:488	arg1	composite					503:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	0	41	theme	bio-composite	54:66	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of substituted hydroxyapatite-starch-clay bio-composite	0:66	Fabrication of substituted hydroxyapatite-starch-clay bio-composite coated titanium implant for new bone formation.					
34364572	1	42	dep	numerous	222:229	arg1	uses					231:234	uses	231:234	uses	231:234	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	3	43	theme	clay	425:428	arg1	minerals					439:446	Wollastonite (WST) clay combined minerals	406:446	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	5	44	theme	SEM	796:798	arg1	analysis					800:807	SEM analysis	796:807	SEM analysis	796:807	The functionality, phase, morphology, and bio-activity analysis of the composite were evaluated by FT-IR, XRD, HR-TEM, and SEM analysis, respectively.					
34364572	2	45	theme	new	259:261	arg1	implant					288:294	new orthopedic titanium (Ti) implant	259:294	new orthopedic titanium (Ti) implant	259:294	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	7	46	theme	Ca	1023:1024	arg1	mineralization					1026:1039	Ca mineralization	1023:1039	Ca mineralization	1023:1039	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	0	47	theme	titanium	75:82	arg1	implant					84:90	titanium implant	75:90	titanium implant	75:90	Fabrication of substituted hydroxyapatite-starch-clay bio-composite coated titanium implant for new bone formation.					
34364572	7	48	theme	composite	1060:1068	arg1	viability					991:999	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	7	48	theme	composite	1060:1068	arg1	mineralization					1026:1039	Ca mineralization	1023:1039	Ca mineralization	1023:1039	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	7	48	theme	composite	1060:1068	arg1	differentiation					1002:1016	differentiation	1002:1016	differentiation	1002:1016	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	1	49	theme	much	149:152	arg1	attention					154:162	much attention	149:162	much attention from researchers	149:179	The clay/polymeric matrices have much attention from researchers in bio-medical applications due to their numerous uses.					
34364572	7	50	theme	in-vivo	1176:1182	arg1	evaluation					1162:1171	careful evaluation	1154:1171	careful evaluation of in-vivo and clinical studies	1154:1203	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	7	51	theme	careful	1154:1160	arg1	evaluation					1162:1171	careful evaluation	1154:1171	careful evaluation of in-vivo and clinical studies	1154:1203	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	2	52	theme	orthopedic	263:272	arg1	implant					288:294	new orthopedic titanium (Ti) implant	259:294	new orthopedic titanium (Ti) implant	259:294	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	2	53	theme	Ti	356:357	arg1	implant					359:365	the Ti implant	352:365	the Ti implant	352:365	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	4	54	theme	Electrophoretic	632:646	arg1	method					665:670	the Electrophoretic Deposition (EPD) method	628:670	the Electrophoretic Deposition (EPD) method	628:670	It was successfully coated on the orthopedic grade Ti plate by the Electrophoretic Deposition (EPD) method.					
34364572	6	55	theme	mechanical	828:837	arg1	property					839:846	The mechanical property	824:846	The mechanical property	824:846	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	3	56	theme	/Starch	495:501	arg1	composite					503:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite	406:511	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	0	57	theme	new	96:98	arg1	formation					105:113	new bone formation	96:113	new bone formation	96:113	Fabrication of substituted hydroxyapatite-starch-clay bio-composite coated titanium implant for new bone formation.					
34364572	2	58	theme	Ti	284:285	arg1	implant					288:294	new orthopedic titanium (Ti) implant	259:294	new orthopedic titanium (Ti) implant	259:294	This study introduces new orthopedic titanium (Ti) implant with increasing bio-activity by treating the surface of the Ti implant with bio-compatible composite coating.					
34364572	3	59	theme	co-precipitation	540:555	arg1	method					557:562	in-situ co-precipitation method	532:562	in-situ co-precipitation method	532:562	Wollastonite (WST) clay combined minerals (Mg2+and Gd3+) substituted hydroxyapatite (HAP)/Starch composite was prepared using in-situ co-precipitation method.					
34364572	6	60	theme	composite	906:914	arg1	plate					926:930	the MHAP/Starch/WST composite coated Ti plate	886:930	the MHAP/Starch/WST composite coated Ti plate	886:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	6	61	theme	plate	926:930	arg1	value					877:881	Vickers microhardness value	855:881	Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate	855:930	The mechanical property, i.e., Vickers microhardness value of the MHAP/Starch/WST composite coated Ti plate, showed 242 ± 1.92 Hv.					
34364572	7	62	theme	osteoblast	974:983	arg1	cells					985:989	The in-vitro MG-63 osteoblast cells	955:989	The in-vitro MG-63 osteoblast cells viability	955:999	The in-vitro MG-63 osteoblast cells viability, differentiation, and Ca mineralization of MHAP/Starch/WST composite suggests that this new implant will be used for bone regeneration application after careful evaluation of in-vivo and clinical studies.					
34364572	4	63	theme	Deposition	648:657	arg1	method					665:670	the Electrophoretic Deposition (EPD) method	628:670	the Electrophoretic Deposition (EPD) method	628:670	It was successfully coated on the orthopedic grade Ti plate by the Electrophoretic Deposition (EPD) method.					
32393048	5	0	theme	receptor	740:747	arg1	signaling					749:757	cell receptor signaling	735:757	cell receptor signaling	735:757	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	8	1	theme	clinical	1161:1168	arg1	trials					1170:1175	More controlled clinical trials	1145:1175	More controlled clinical trials	1145:1175	More controlled clinical trials are needed to justify routine supplementation of formula.					
32393048	5	2	theme	gut	668:670	arg1	microbiota					672:681	infant gut microbiota	661:681	infant gut microbiota	661:681	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	1	3	theme	bioactive	213:221	arg1	components					223:232	many distinct bioactive components	199:232	many distinct bioactive components	199:232	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	8	4	theme	controlled	1150:1159	arg1	trials					1170:1175	More controlled clinical trials	1145:1175	More controlled clinical trials	1145:1175	More controlled clinical trials are needed to justify routine supplementation of formula.					
32393048	0	5	theme	milk	89:92	arg1	effects					71:77	the beneficial effects	56:77	the beneficial effects of breast milk	56:92	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	2	6	theme	milk	254:257	arg1	"					365:365	a "novel foods"	351:365	a "novel foods"	351:365	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	2	6	theme	milk	254:257	arg1	HMOs					277:280	HMOs	277:280	HMOs	277:280	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	2	6	theme	milk	254:257	arg1	oligosaccharides					259:274	human milk oligosaccharides	248:274	human milk oligosaccharides (HMOs)	248:281	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	6	7	theme	necrotizing	967:977	arg1	enterocolitis					979:991	necrotizing enterocolitis	967:991	necrotizing enterocolitis	967:991	These studies, combined with epidemiological data, indicate that some HMO may confer health benefits by preventing infections and diseases such as necrotizing enterocolitis and allergies.					
32393048	0	8	theme	breast	82:87	arg1	milk					89:92	breast milk	82:92	breast milk	82:92	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	2	9	theme	human	248:252	arg1	"					365:365	a "novel foods"	351:365	a "novel foods"	351:365	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	2	9	theme	human	248:252	arg1	HMOs					277:280	HMOs	277:280	HMOs	277:280	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	2	9	theme	human	248:252	arg1	oligosaccharides					259:274	human milk oligosaccharides	248:274	human milk oligosaccharides (HMOs)	248:281	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	0	10	from	improvement	118:128	arg1	formulas					140:147	infant formulas	133:147	infant formulas	133:147	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	4	11	theme	Recent	425:430	arg1	research					432:439	Recent research	425:439	Recent research	425:439	Recent research has focused on the complexity of HMOs by highlighting their diversity, structural variability, concentration variance, and structure-function relationships.					
32393048	5	12	theme	In	598:599	arg1	studies					619:625	In vitro and in vivo studies	598:625	In vitro and in vivo studies	598:625	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	9	13	theme	role	1281:1284	arg1	understanding					1260:1272	a better understanding	1251:1272	a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production	1251:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	2	14	theme	novel	354:358	arg1	"					365:365	a "novel foods"	351:365	a "novel foods"	351:365	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	2	14	theme	novel	354:358	arg1	oligosaccharides					259:274	human milk oligosaccharides	248:274	human milk oligosaccharides (HMOs)	248:281	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	4	15	theme	concentration	536:548	arg1	variance					550:557	concentration variance	536:557	concentration variance	536:557	Recent research has focused on the complexity of HMOs by highlighting their diversity, structural variability, concentration variance, and structure-function relationships.					
32393048	1	16	theme	Human	150:154	arg1	milk					156:159	Human milk	150:159	Human milk	150:159	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	1	16	theme	Human	150:154	arg1	source					166:171	a source	164:171	a source of nutrients	164:184	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	8	17	theme	routine	1199:1205	arg1	supplementation					1207:1221	routine supplementation	1199:1221	routine supplementation of formula	1199:1232	More controlled clinical trials are needed to justify routine supplementation of formula.					
32393048	9	18	theme	HMO	1360:1362	arg1	production					1364:1373	large-scale HMO production	1348:1373	large-scale HMO production	1348:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	8	19	theme	More	1145:1148	arg1	trials					1170:1175	More controlled clinical trials	1145:1175	More controlled clinical trials	1145:1175	More controlled clinical trials are needed to justify routine supplementation of formula.					
32393048	9	20	theme	methods	1336:1342	arg1	development					1309:1319	the development	1305:1319	the development of inexpensive methods for large-scale HMO production	1305:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	5	21	theme	in vivo	611:617	arg1	studies					619:625	In vitro and in vivo studies	598:625	In vitro and in vivo studies	598:625	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	5	22	theme	barrier	703:709	arg1	functions					711:719	intestinal barrier functions	692:719	intestinal barrier functions	692:719	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	2	23	theme	foods	360:364	arg1	"					365:365	a "novel foods"	351:365	a "novel foods"	351:365	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	2	23	theme	foods	360:364	arg1	oligosaccharides					259:274	human milk oligosaccharides	248:274	human milk oligosaccharides (HMOs)	248:281	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	5	24	theme	infant	803:808	arg1	immunity					810:817	infant immunity	803:817	infant immunity	803:817	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	9	25	theme	large-scale	1348:1358	arg1	production					1364:1373	large-scale HMO production	1348:1373	large-scale HMO production	1348:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	7	26	theme	simple	1077:1082	arg1	fucosyllactose					1105:1118	fucosyllactose	1105:1118	fucosyllactose	1105:1118	However, randomized controlled trials are restricted to structurally simple compounds such as 2' fucosyllactose and lacto-N-neotetraose.					
32393048	7	26	theme	simple	1077:1082	arg1	compounds					1084:1092	structurally simple compounds	1064:1092	structurally simple compounds such as 2' fucosyllactose and lacto-N-neotetraose	1064:1142	However, randomized controlled trials are restricted to structurally simple compounds such as 2' fucosyllactose and lacto-N-neotetraose.					
32393048	7	26	theme	simple	1077:1082	arg1	lacto-N-neotetraose					1124:1142	lacto-N-neotetraose	1124:1142	lacto-N-neotetraose	1124:1142	However, randomized controlled trials are restricted to structurally simple compounds such as 2' fucosyllactose and lacto-N-neotetraose.					
32393048	6	27	theme	epidemiological	849:863	arg1	data					865:868	epidemiological data	849:868	epidemiological data	849:868	These studies, combined with epidemiological data, indicate that some HMO may confer health benefits by preventing infections and diseases such as necrotizing enterocolitis and allergies.					
32393048	7	28	theme	controlled	1028:1037	arg1	trials					1039:1044	randomized controlled trials	1017:1044	randomized controlled trials	1017:1044	However, randomized controlled trials are restricted to structurally simple compounds such as 2' fucosyllactose and lacto-N-neotetraose.					
32393048	0	29	theme	milk	31:34	arg1	oligosaccharides					36:51	human milk oligosaccharides	25:51	human milk oligosaccharides	25:51	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	4	30	theme	structural	512:521	arg1	variability					523:533	structural variability	512:533	structural variability	512:533	Recent research has focused on the complexity of HMOs by highlighting their diversity, structural variability, concentration variance, and structure-function relationships.					
32393048	5	31	theme	intestinal	692:701	arg1	functions					711:719	intestinal barrier functions	692:719	intestinal barrier functions	692:719	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	0	32	theme	human	25:29	arg1	oligosaccharides					36:51	human milk oligosaccharides	25:51	human milk oligosaccharides	25:51	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	9	33	theme	HMOs	1289:1292	arg1	role					1281:1284	the role	1277:1284	the role of HMOs leading to the development of inexpensive methods for large-scale HMO production	1277:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	3	34	theme	Human	368:372	arg1	milk					374:377	Human milk	368:377	Human milk	368:377	Human milk is unique in its oligosaccharide composition.					
32393048	3	35	theme	oligosaccharide	396:410	arg1	composition					412:422	its oligosaccharide composition	392:422	its oligosaccharide composition	392:422	Human milk is unique in its oligosaccharide composition.					
32393048	5	36	dep	In	598:599	arg1	vitro					601:605	vitro	601:605	vitro	601:605	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	4	37	theme	HMOs	474:477	arg1	complexity					460:469	the complexity	456:469	the complexity of HMOs	456:477	Recent research has focused on the complexity of HMOs by highlighting their diversity, structural variability, concentration variance, and structure-function relationships.					
32393048	8	38	theme	formula	1226:1232	arg1	supplementation					1207:1221	routine supplementation	1199:1221	routine supplementation of formula	1199:1232	More controlled clinical trials are needed to justify routine supplementation of formula.					
32393048	9	39	theme	better	1253:1258	arg1	understanding					1260:1272	a better understanding	1251:1272	a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production	1251:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	1	40	contain	contains	190:197	arg1	milk					156:159	Human milk	150:159	Human milk	150:159	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	1	40	contain	contains	190:197	arg1	source					166:171	a source	164:171	a source of nutrients	164:184	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	1	40	contain	contains	190:197	arg2	components					223:232	many distinct bioactive components	199:232	many distinct bioactive components	199:232	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	7	41	theme	randomized	1017:1026	arg1	trials					1039:1044	randomized controlled trials	1017:1044	randomized controlled trials	1017:1044	However, randomized controlled trials are restricted to structurally simple compounds such as 2' fucosyllactose and lacto-N-neotetraose.					
32393048	0	42	theme	oligosaccharides	36:51	arg1	contribution					9:20	the contribution	5:20	the contribution of human milk oligosaccharides to the beneficial effects of breast milk	5:92	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	3	43	from	composition	412:422	arg1	unique					382:387	unique	382:387	unique	382:387	Human milk is unique in its oligosaccharide composition.					
32393048	2	44	theme	considerable	298:309	arg1	attention					311:319	considerable attention	298:319	considerable attention	298:319	Among these, human milk oligosaccharides (HMOs) have attracted considerable attention and are being investigated as a "novel foods".					
32393048	6	45	theme	health	905:910	arg1	benefits					912:919	health benefits	905:919	health benefits	905:919	These studies, combined with epidemiological data, indicate that some HMO may confer health benefits by preventing infections and diseases such as necrotizing enterocolitis and allergies.					
32393048	5	46	theme	infant	661:666	arg1	microbiota					672:681	infant gut microbiota	661:681	infant gut microbiota	661:681	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	4	47	theme	structure-function	564:581	arg1	relationships					583:595	structure-function relationships	564:595	structure-function relationships	564:595	Recent research has focused on the complexity of HMOs by highlighting their diversity, structural variability, concentration variance, and structure-function relationships.					
32393048	0	48	theme	infant	133:138	arg1	formulas					140:147	infant formulas	133:147	infant formulas	133:147	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	0	49	theme	beneficial	60:69	arg1	effects					71:77	the beneficial effects	56:77	the beneficial effects of breast milk	56:92	Does the contribution of human milk oligosaccharides to the beneficial effects of breast milk allow us to hope for an improvement in infant formulas?					
32393048	1	50	theme	nutrients	176:184	arg1	milk					156:159	Human milk	150:159	Human milk	150:159	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	1	50	theme	nutrients	176:184	arg1	source					166:171	a source	164:171	a source of nutrients	164:184	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	5	51	theme	immunity	810:817	arg1	development					788:798	the development	784:798	the development of infant immunity	784:817	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	1	52	theme	many	199:202	arg1	components					223:232	many distinct bioactive components	199:232	many distinct bioactive components	199:232	Human milk is a source of nutrients and contains many distinct bioactive components.					
32393048	5	53	theme	cell	735:738	arg1	signaling					749:757	cell receptor signaling	735:757	cell receptor signaling	735:757	In vitro and in vivo studies have demonstrated that HMOs drive infant gut microbiota, improve intestinal barrier functions, and modulate cell receptor signaling, thereby contributing to the development of infant immunity.					
32393048	3	54	from	unique	382:387	arg1	composition					412:422	its oligosaccharide composition	392:422	its oligosaccharide composition	392:422	Human milk is unique in its oligosaccharide composition.					
32393048	9	55	theme	inexpensive	1324:1334	arg1	methods					1336:1342	inexpensive methods	1324:1342	inexpensive methods for large-scale HMO production	1324:1373	It is felt that a better understanding of the role of HMOs leading to the development of inexpensive methods for large-scale HMO production is needed.					
32393048	1	56	theme	distinct	204:211	arg1	components					223:232	many distinct bioactive components	199:232	many distinct bioactive components	199:232	Human milk is a source of nutrients and contains many distinct bioactive components.					
34582985	4	0	dep	pollutants	1061:1070	arg1	MB					1073:1074	MB	1073:1074	MB	1073:1074	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	4	0	dep	pollutants	1061:1070	arg1	pollutants					1061:1070	the two pollutants	1053:1070	the two pollutants (MB and Hg(II))	1053:1086	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	4	0	dep	pollutants	1061:1070	arg1	II					1083:1084	II	1083:1084	II	1083:1084	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	4	0	dep	pollutants	1061:1070	arg1	Hg					1080:1081	Hg	1080:1081	Hg(II)	1080:1085	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	1	1	link	stalks-derived	395:408	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	1	link	stalks-derived	395:408	arg1	AGB					419:421	AGB	419:421	AGB	419:421	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	1	link	stalks-derived	395:408	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	4	2	with	equation	1134:1141	arg1	participation					1157:1169	possible participation	1148:1169	possible participation of intraparticle diffusion mechanism	1148:1206	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	3	3	theme	MB	940:941	arg1	dye					943:945	10mgL-1 MB dye	932:945	10mgL-1 MB dye	932:945	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	1	4	theme	species	587:593	arg1	removal					559:565	removal	559:565	removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater	559:653	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	5	theme	Zn-Al	349:353	arg1	hydroxide					338:346	layered double hydroxide	323:346	layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	323:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	5	theme	Zn-Al	349:353	arg1	LDHs					355:358	Zn-Al LDHs	349:358	Zn-Al LDHs	349:358	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	6	theme	MnFe2O4	425:431	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	6	theme	MnFe2O4	425:431	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	7	theme	biochar	86:92	arg1	nanocomposite					94:106	biochar nanocomposite	86:106	biochar nanocomposite for efficient remediation of methylene blue and mercury (II)	86:167	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	4	8	theme	mechanism	1198:1206	arg1	participation					1157:1169	possible participation	1148:1169	possible participation of intraparticle diffusion mechanism	1148:1206	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	1	9	theme	@	432:432	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	9	theme	@	432:432	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	10	theme	fantastic	496:504	arg1	features					506:513	fantastic features	496:513	fantastic features	496:513	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	11	theme	efficient	112:120	arg1	remediation					122:132	efficient remediation	112:132	efficient remediation of methylene blue and mercury (II)	112:167	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	4	12	theme	diffusion	1188:1196	arg1	mechanism					1198:1206	intraparticle diffusion mechanism	1174:1206	intraparticle diffusion mechanism	1174:1206	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	3	13	theme	30 min	980:985	arg1	times					995:999	25 and 30 min contact times	973:999	times	995:999	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	1	14	theme	cationic	578:585	arg1	species					587:593	harmful cationic species	570:593	harmful cationic species as methylene blue dye (MB)	570:620	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	14	theme	cationic	578:585	arg1	MB					618:619	MB	618:619	MB	618:619	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	2	15	theme	surface	696:702	arg1	area					704:707	surface area	696:707	surface area	696:707	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	1	16	theme	mercury	626:632	arg1	ions					634:637	mercury ions	626:637	mercury ions	626:637	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	3	17	theme	contact	987:993	arg1	times					995:999	25 and 30 min contact times	973:999	times	995:999	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	1	18	theme	LDHs	439:442	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	18	theme	LDHs	439:442	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	19	theme	blue	608:611	arg1	dye					613:615	methylene blue dye	598:615	methylene blue dye	598:615	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	3	20	theme	10mgL-1	932:938	arg1	dye					943:945	10mgL-1 MB dye	932:945	10mgL-1 MB dye	932:945	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	0	21	theme	Mn-ferrite	10:19	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	3	22	theme	25	973:974	arg1	times					995:999	25 and 30 min contact times	973:999	times	995:999	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	1	23	theme	@	443:443	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	23	theme	@	443:443	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	24	theme	Decorated	0:8	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	25	theme	magnetic	275:282	arg1	nanoparticle					295:306	magnetic Mn-ferrite nanoparticle	275:306	magnetic Mn-ferrite nanoparticle (MnFe2O4)	275:316	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	25	theme	magnetic	275:282	arg1	MnFe2O4					309:315	MnFe2O4	309:315	MnFe2O4	309:315	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	26	theme	Cel	444:446	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	26	theme	Cel	444:446	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	4	27	theme	pseudo-second-order	1114:1132	arg1	equation					1134:1141	the pseudo-second-order equation	1110:1141	the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism	1110:1206	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	1	28	from	hydroxide	338:346	arg1	grapes					388:393	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	28	from	hydroxide	338:346	arg1	cellulose					364:372	cellulose	364:372	cellulose	364:372	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	29	theme	innovative	173:182	arg1	nanocomposite					193:205	An innovative magnetic nanocomposite	170:205	An innovative magnetic nanocomposite	170:205	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	30	theme	@	33:33	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	31	theme	Mn-ferrite	284:293	arg1	nanoparticle					295:306	magnetic Mn-ferrite nanoparticle	275:306	magnetic Mn-ferrite nanoparticle (MnFe2O4)	275:316	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	31	theme	Mn-ferrite	284:293	arg1	MnFe2O4					309:315	MnFe2O4	309:315	MnFe2O4	309:315	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	32	theme	@	447:447	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	32	theme	@	447:447	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	33	theme	magnetic	184:191	arg1	nanocomposite					193:205	An innovative magnetic nanocomposite	170:205	An innovative magnetic nanocomposite	170:205	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	34	theme	nanoparticle	21:32	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	35	theme	Zn-Al	433:437	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	35	theme	Zn-Al	433:437	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	2	36	theme	adsorption	710:719	arg1	performance					721:731	adsorption performance	710:731	adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability	710:805	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	3	37	theme	ofthe	863:867	arg1	nanocomposite					869:881	10 mg ofthe nanocomposite	857:881	10 mg ofthe nanocomposite	857:881	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	0	38	theme	layered	40:46	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	2	39	dep	Structural	656:665	arg1	morphological					681:693	morphological	681:693	morphological	681:693	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	2	39	dep	Structural	656:665	arg1	area					704:707	surface area	696:707	surface area	696:707	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	2	39	dep	Structural	656:665	arg1	composition					668:678	composition	668:678	composition	668:678	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	0	40	theme	blue	147:150	arg1	methylene					137:145	methylene blue	137:150	methylene blue	137:150	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	41	theme	activated	378:386	arg1	grapes					388:393	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	4	42	theme	possible	1148:1155	arg1	participation					1157:1169	possible participation	1148:1169	possible participation of intraparticle diffusion mechanism	1148:1206	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	3	43	theme	10 mg	857:861	arg1	nanocomposite					869:881	10 mg ofthe nanocomposite	857:881	10 mg ofthe nanocomposite	857:881	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	0	44	theme	Zn-Al	34:38	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	0	45	theme	methylene	137:145	arg1	remediation					122:132	efficient remediation	112:132	efficient remediation of methylene blue and mercury (II)	112:167	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	2	46	dep	investigated	817:828	arg1	Structural					656:665	Structural	656:665	Structural	656:665	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	0	47	theme	hydroxide	55:63	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	4	48	theme	Adsorption	1016:1025	arg1	isotherms					1027:1035	Adsorption isotherms	1016:1035	Adsorption isotherms	1016:1035	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	0	49	theme	mercury	156:162	arg1	remediation					122:132	efficient remediation	112:132	efficient remediation of methylene blue and mercury (II)	112:167	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	50	theme	stalks-derived	395:408	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	50	theme	stalks-derived	395:408	arg1	AGB					419:421	AGB	419:421	AGB	419:421	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	50	theme	stalks-derived	395:408	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	51	theme	ions	634:637	arg1	removal					559:565	removal	559:565	removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater	559:653	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	52	theme	double	48:53	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	53	from	wastewater	644:653	arg1	removal					559:565	removal	559:565	removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater	559:653	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	4	54	theme	intraparticle	1174:1186	arg1	mechanism					1198:1206	intraparticle diffusion mechanism	1174:1206	intraparticle diffusion mechanism	1174:1206	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	1	55	theme	layered	323:329	arg1	hydroxide					338:346	layered double hydroxide	323:346	layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	323:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	55	theme	layered	323:329	arg1	LDHs					355:358	Zn-Al LDHs	349:358	Zn-Al LDHs	349:358	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	3	56	theme	L-1	959:961	arg1	II					966:967	II	966:967	II	966:967	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	56	theme	L-1	959:961	arg1	Hg					963:964	0.1 mol L-1 Hg	951:964	0.1 mol L-1 Hg(II)	951:968	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	57	theme	dye	943:945	arg1	%					897:897	97.4%	893:897	97.4%	893:897	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	57	theme	dye	943:945	arg1	%					908:908	84.0 %	903:908	84.0 %	903:908	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	57	theme	dye	943:945	arg1	dye					943:945	10mgL-1 MB dye	932:945	10mgL-1 MB dye	932:945	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	57	theme	dye	943:945	arg1	II					966:967	II	966:967	II	966:967	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	57	theme	dye	943:945	arg1	Hg					963:964	0.1 mol L-1 Hg	951:964	0.1 mol L-1 Hg(II)	951:968	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	58	theme	0.1 mol	951:957	arg1	II					966:967	II	966:967	II	966:967	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	3	58	theme	0.1 mol	951:957	arg1	Hg					963:964	0.1 mol L-1 Hg	951:964	0.1 mol L-1 Hg(II)	951:968	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	2	59	theme	nanocomposite	751:763	arg1	performance					721:731	adsorption performance	710:731	adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability	710:805	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	1	60	dep	grapes	388:393	arg1	AGB					448:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	MnFe2O4@Zn-Al LDHs@Cel@AGB	425:450	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	60	dep	grapes	388:393	arg1	AGB					419:421	AGB	419:421	AGB	419:421	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	60	dep	grapes	388:393	arg1	biochar					410:416	stalks-derived biochar	395:416	activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	378:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	3	61	theme	removal	910:916	arg1	efficiency					918:927	removal efficiency	910:927	removal efficiency	910:927	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	2	62	theme	fabricated	740:749	arg1	nanocomposite					751:763	the fabricated nanocomposite	736:763	the fabricated nanocomposite toward both MB and Hg(II) and reusability	736:805	Structural, composition, morphological, surface area, adsorption performance of the fabricated nanocomposite toward both MB and Hg(II) and reusability were also investigated.					
34582985	3	63	dep	%	897:897	arg1	efficiency					918:927	removal efficiency	910:927	removal efficiency	910:927	The results referred that 10 mg ofthe nanocomposite exhibited 97.4% and 84.0 % removal efficiency of 10mgL-1 MB dye and 0.1 mol L-1 Hg(II) at 25 and 30 min contact times, respectively.					
34582985	0	64	theme	Cellulose	65:73	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	65	theme	active	469:474	arg1	functionalities					476:490	active functionalities	469:490	active functionalities	469:490	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	0	66	theme	@	64:64	arg1	@					74:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@	0:74	Decorated Mn-ferrite nanoparticle@Zn-Al layered double hydroxide@Cellulose@ activated biochar nanocomposite for efficient remediation of methylene blue and mercury (II).					
34582985	1	67	theme	double	331:336	arg1	hydroxide					338:346	layered double hydroxide	323:346	layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB)	323:451	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	67	theme	double	331:336	arg1	LDHs					355:358	Zn-Al LDHs	349:358	Zn-Al LDHs	349:358	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	4	68	theme	pollutants	1061:1070	arg1	isotherms					1027:1035	Adsorption isotherms	1016:1035	Adsorption isotherms	1016:1035	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	4	68	theme	pollutants	1061:1070	arg1	kinetics					1041:1048	kinetics	1041:1048	kinetics	1041:1048	Adsorption isotherms and kinetics of the two pollutants (MB and Hg(II)) were both governed by the pseudo-second-order equation with possible participation of intraparticle diffusion mechanism.					
34582985	1	69	theme	methylene	598:606	arg1	dye					613:615	methylene blue dye	598:615	methylene blue dye	598:615	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	70	theme	harmful	570:576	arg1	species					587:593	harmful cationic species	570:593	harmful cationic species as methylene blue dye (MB)	570:620	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
34582985	1	70	theme	harmful	570:576	arg1	MB					618:619	MB	618:619	MB	618:619	An innovative magnetic nanocomposite was designed and fabricated by the functionalization and support of magnetic Mn-ferrite nanoparticle (MnFe2O4) with layered double hydroxide (Zn-Al LDHs) on cellulose and activated grapes stalks-derived biochar (AGB) (MnFe2O4@Zn-Al LDHs@Cel@AGB), to incorporate active functionalities and fantastic features with the aim to explore its feasibility for removal of harmful cationic species as methylene blue dye (MB) and mercury ions from wastewater.					
32497167	4	0	theme	LMWH	765:768	arg1	doses					756:760	capped doses	749:760	capped doses of LMWH	749:768	A total of 2846 patients who weighed >100 kg were included: 454 (16%) received capped doses of LMWH, and the remaining 2392 received uncapped doses.					
32497167	2	1	theme	LMWH	528:531	arg1	IU/d					541:544	18 000 IU/d	534:544	18 000 IU/d	534:544	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	1	theme	LMWH	528:531	arg1	doses					519:523	capped doses	512:523	capped doses of LMWH (18 000 IU/d)	512:545	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	8	2	theme	composite	1355:1363	arg1	outcome					1365:1371	the composite outcome	1351:1371	the composite outcome	1351:1371	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	1	3	theme	heparin	265:271	arg1	dosing					234:239	the dosing	230:239	the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE)	230:331	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	7	4	theme	%	1205:1205	arg1	interval					1218:1225	95% confidence interval [CI]	1203:1230	95% confidence interval [CI]	1203:1230	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	8	5	contain	had	1318:1320	arg1	patients					1283:1290	patients	1283:1290	patients who received capped doses	1283:1316	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	8	5	contain	had	1318:1320	arg2	rates					1342:1346	significantly lower rates	1322:1346	significantly lower rates of the composite outcome	1322:1371	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	6	6	theme	bleeding	983:990	arg1	65					1002:1003	65	1002:1003	65	1002:1003	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	6	theme	bleeding	983:990	arg1	episodes					992:999	bleeding episodes	983:999	bleeding episodes	983:999	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	9	7	theme	composite	1607:1615	arg1	rate					1623:1626	the significantly lower composite event rate	1583:1626	the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death	1583:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	2	8	theme	uncapped	550:557	arg1	doses					559:563	uncapped doses	550:563	uncapped doses (>18 000 IU/d)	550:578	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	8	theme	uncapped	550:557	arg1	IU/d					574:577	>18 000 IU/d	566:577	>18 000 IU/d	566:577	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	1	9	with	patients	290:297	arg1	VTE					328:330	VTE	328:330	VTE	328:330	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	1	9	with	patients	290:297	arg1	thromboembolism					311:325	venous thromboembolism	304:325	venous thromboembolism (VTE)	304:331	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	9	10	theme	VTE	1631:1633	arg1	recurrence					1635:1644	VTE recurrence	1631:1644	VTE recurrence	1631:1644	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	8	11	dep	had	1318:1320	arg1	odds					1374:1377	odds	1374:1377	odds	1374:1377	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	1	12	theme	product	200:206	arg1	monographs					208:217	product monographs	200:217	product monographs	200:217	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	2	13	theme	LMWH	494:497	arg1	therapy					499:505	LMWH therapy	494:505	LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d)	494:578	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	0	14	from	patients	89:96	arg1	treatment					50:58	treatment	50:58	treatment of venous thromboembolism in patients with obesity (RIETE registry)	50:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	6	15	theme	outcomes	1065:1072	arg1	1					1046:1046	1	1046:1046	1	1046:1046	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	15	theme	outcomes	1065:1072	arg1	outcomes					1065:1072	the composite outcomes	1051:1072	the composite outcomes	1051:1072	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	4	16	theme	remaining	779:787	arg1	2392					789:792	the remaining 2392	775:792	the remaining 2392	775:792	A total of 2846 patients who weighed >100 kg were included: 454 (16%) received capped doses of LMWH, and the remaining 2392 received uncapped doses.					
32497167	0	17	from	thromboembolism	70:84	arg1	patients					89:96	patients	89:96	patients with obesity (RIETE registry)	89:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	9	18	theme	bleeding	1653:1660	arg1	rate					1623:1626	the significantly lower composite event rate	1583:1626	the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death	1583:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	5	19	theme	Mean	819:822	arg1	deviation					834:842	standard deviation	825:842	standard deviation	825:842	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	5	19	theme	Mean	819:822	arg1	duration					860:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	9	20	theme	retrospective	1439:1451	arg1	data					1467:1470	These retrospective observational data	1433:1470	These retrospective observational data	1433:1470	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	6	21	contain	had	948:950	arg2	recurrences					956:966	VTE recurrences	952:966	VTE recurrences	952:966	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	21	contain	had	948:950	arg1	patients					932:939	Thirty-one patients	921:939	Thirty-one patients (1.9%)	921:946	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	21	contain	had	948:950	arg1	%					945:945	1.9%	942:945	1.9%	942:945	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	9	22	theme	death	1677:1681	arg1	rate					1623:1626	the significantly lower composite event rate	1583:1626	the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death	1583:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	0	23	theme	thromboembolism	70:84	arg1	treatment					50:58	treatment	50:58	treatment of venous thromboembolism in patients with obesity (RIETE registry)	50:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	8	24	dep	odds	1374:1377	arg1	%					1394:1394	95% CI	1392:1397	95% CI	1392:1397	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	8	24	dep	odds	1374:1377	arg1	ratio					1379:1383	ratio	1379:1383	ratio	1379:1383	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	8	24	dep	odds	1374:1377	arg1	0.04-0.68					1400:1408	0.04-0.68	1400:1408	0.04-0.68	1400:1408	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	2	25	with	patients	449:456	arg1	VTE					463:465	VTE	463:465	VTE	463:465	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	1	26	theme	comparative	155:165	arg1	evidence					167:174	comparative evidence	155:174	comparative evidence	155:174	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	5	27	theme	treatment	850:858	arg1	deviation					834:842	standard deviation	825:842	standard deviation	825:842	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	5	27	theme	treatment	850:858	arg1	duration					860:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	2	28	theme	primary	376:382	arg1	outcomes					394:401	the primary composite outcomes	372:401	the primary composite outcomes (VTE recurrence, major bleeding, or death)	372:444	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	28	theme	primary	376:382	arg1	recurrence					408:417	VTE recurrence	404:417	VTE recurrence	404:417	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	28	theme	primary	376:382	arg1	death					439:443	death	439:443	death	439:443	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	0	29	theme	RIETE	112:116	arg1	obesity					103:109	obesity	103:109	obesity (RIETE registry)	103:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	0	29	theme	RIETE	112:116	arg1	registry					118:125	RIETE registry	112:125	RIETE registry	112:125	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	7	30	theme	outcome	1086:1092	arg1	rates					1094:1098	Unadjusted outcome rates	1075:1098	Unadjusted outcome rates	1075:1098	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	9	31	theme	LMWH	1502:1505	arg1	dosing					1492:1497	capped dosing	1485:1497	capped dosing of LMWH	1485:1505	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	9	31	theme	LMWH	1502:1505	arg1	alternative					1524:1534	an acceptable alternative	1510:1534	an acceptable alternative to uncapped dosing based on body weight	1510:1574	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	8	32	theme	Multivariable	1245:1257	arg1	analysis					1259:1266	Multivariable analysis	1245:1266	Multivariable analysis	1245:1266	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	5	33	theme	standard	825:832	arg1	deviation					834:842	standard deviation	825:842	standard deviation	825:842	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	5	33	theme	standard	825:832	arg1	duration					860:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	2	34	theme	VTE	404:406	arg1	outcomes					394:401	the primary composite outcomes	372:401	the primary composite outcomes (VTE recurrence, major bleeding, or death)	372:444	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	34	theme	VTE	404:406	arg1	recurrence					408:417	VTE recurrence	404:417	VTE recurrence	404:417	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	0	35	theme	heparin	31:37	arg1	dosing					39:44	low-molecular-weight heparin dosing	10:44	low-molecular-weight heparin dosing	10:44	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	1	36	theme	obese	284:288	arg1	patients					290:297	obese patients	284:297	obese patients with venous thromboembolism (VTE)	284:331	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	7	37	theme	composite	1166:1174	arg1	outcome					1176:1182	the composite outcome	1162:1182	the composite outcome	1162:1182	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	2	38	dep	outcomes	394:401	arg1	outcomes					394:401	the primary composite outcomes	372:401	the primary composite outcomes (VTE recurrence, major bleeding, or death)	372:444	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	38	dep	outcomes	394:401	arg1	recurrence					408:417	VTE recurrence	404:417	VTE recurrence	404:417	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	38	dep	outcomes	394:401	arg1	bleeding					426:433	bleeding	426:433	bleeding	426:433	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	38	dep	outcomes	394:401	arg1	death					439:443	death	439:443	death	439:443	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	7	39	dep	ratio	1190:1194	arg1	0.04-0.75					1233:1241	0.04-0.75	1233:1241	0.04-0.75	1233:1241	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	7	39	dep	ratio	1190:1194	arg1	interval					1218:1225	95% confidence interval [CI]	1203:1230	95% confidence interval [CI]	1203:1230	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	9	40	theme	uncapped	1539:1546	arg1	dosing					1548:1553	uncapped dosing	1539:1553	uncapped dosing based on body weight	1539:1574	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	5	41	theme	32.3	895:898	arg1	days					901:904	14.8 (20.6) and 14.3 (32.3) days	873:904	14.8 (20.6) and 14.3 (32.3) days	873:904	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	6	42	theme	VTE	952:954	arg1	recurrences					956:966	VTE recurrences	952:966	VTE recurrences	952:966	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	9	43	theme	body	1564:1567	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	3	44	theme	logistic	595:602	arg1	analysis					615:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis was used to account for possible confounders.					
32497167	2	45	with	therapy	499:505	arg1	IU/d					541:544	18 000 IU/d	534:544	18 000 IU/d	534:544	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	45	with	therapy	499:505	arg1	doses					519:523	capped doses	512:523	capped doses of LMWH (18 000 IU/d)	512:545	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	45	with	therapy	499:505	arg1	doses					559:563	uncapped doses	550:563	uncapped doses (>18 000 IU/d)	550:578	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	45	with	therapy	499:505	arg1	IU/d					574:577	>18 000 IU/d	566:577	>18 000 IU/d	566:577	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	7	46	theme	confidence	1207:1216	arg1	interval					1218:1225	95% confidence interval [CI]	1203:1230	95% confidence interval [CI]	1203:1230	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	1	47	theme	low-molecular-weight	244:263	arg1	heparin					265:271	low-molecular-weight heparin	244:271	low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE)	244:331	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	1	47	theme	low-molecular-weight	244:263	arg1	LMWH					274:277	LMWH	274:277	LMWH	274:277	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	0	48	with	patients	89:96	arg1	obesity					103:109	obesity	103:109	obesity (RIETE registry)	103:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	0	48	with	patients	89:96	arg1	registry					118:125	RIETE registry	112:125	RIETE registry	112:125	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	7	49	theme	rate	1185:1188	arg1	0.22					1197:1200	0.22	1197:1200	0.22	1197:1200	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	7	49	theme	rate	1185:1188	arg1	ratio					1190:1194	rate ratio	1185:1194	rate ratio	1185:1194	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	9	50	theme	lower	1601:1605	arg1	rate					1623:1626	the significantly lower composite event rate	1583:1626	the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death	1583:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	8	51	theme	outcome	1365:1371	arg1	rates					1342:1346	significantly lower rates	1322:1346	significantly lower rates of the composite outcome	1322:1371	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	1	52	theme	current	177:183	arg1	guidelines					185:194	current guidelines	177:194	current guidelines	177:194	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	1	53	theme	evidence	167:174	arg1	absence					144:150	the absence	140:150	the absence of comparative evidence	140:174	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	9	54	theme	event	1617:1621	arg1	rate					1623:1626	the significantly lower composite event rate	1583:1626	the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death	1583:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	3	55	theme	possible	648:655	arg1	confounders					657:667	possible confounders	648:667	possible confounders	648:667	Multivariable logistic regression analysis was used to account for possible confounders.					
32497167	7	56	dep	associated	1132:1141	arg1	0.22					1197:1200	0.22	1197:1200	0.22	1197:1200	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	7	56	dep	associated	1132:1141	arg1	ratio					1190:1194	rate ratio	1185:1194	rate ratio	1185:1194	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	4	57	theme	capped	749:754	arg1	doses					756:760	capped doses	749:760	capped doses of LMWH	749:768	A total of 2846 patients who weighed >100 kg were included: 454 (16%) received capped doses of LMWH, and the remaining 2392 received uncapped doses.					
32497167	4	58	theme	uncapped	803:810	arg1	doses					812:816	uncapped doses	803:816	uncapped doses	803:816	A total of 2846 patients who weighed >100 kg were included: 454 (16%) received capped doses of LMWH, and the remaining 2392 received uncapped doses.					
32497167	9	59	theme	recurrence	1635:1644	arg1	rate					1623:1626	the significantly lower composite event rate	1583:1626	the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death	1583:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	6	60	theme	composite	1055:1063	arg1	outcomes					1065:1072	the composite outcomes	1051:1072	the composite outcomes	1051:1072	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	7	61	from	decrease	1150:1157	arg1	outcome					1176:1182	the composite outcome	1162:1182	the composite outcome	1162:1182	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	2	62	theme	capped	512:517	arg1	IU/d					541:544	18 000 IU/d	534:544	18 000 IU/d	534:544	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	62	theme	capped	512:517	arg1	doses					519:523	capped doses	512:523	capped doses of LMWH (18 000 IU/d)	512:545	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	7	63	theme	capped	1114:1119	arg1	dosing					1121:1126	capped dosing	1114:1126	capped dosing	1114:1126	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	9	64	theme	all-cause	1667:1675	arg1	death					1677:1681	all-cause death	1667:1681	all-cause death	1667:1681	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	9	65	theme	observational	1453:1465	arg1	data					1467:1470	These retrospective observational data	1433:1470	These retrospective observational data	1433:1470	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	3	66	used	used	628:631	arg2	analysis					615:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis was used to account for possible confounders.					
32497167	2	67	used	used	337:340	arg2	We					334:335	We	334:335	We	334:335	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	5	68	theme	LMWH	845:848	arg1	deviation					834:842	standard deviation	825:842	standard deviation	825:842	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	5	68	theme	LMWH	845:848	arg1	duration					860:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration	819:867	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	9	69	theme	capped	1485:1490	arg1	dosing					1492:1497	capped dosing	1485:1497	capped dosing of LMWH	1485:1505	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	9	69	theme	capped	1485:1490	arg1	alternative					1524:1534	an acceptable alternative	1510:1534	an acceptable alternative to uncapped dosing based on body weight	1510:1574	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	4	70	theme	patients	686:693	arg1	total					672:676	A total	670:676	A total of 2846 patients who weighed >100 kg	670:713	A total of 2846 patients who weighed >100 kg were included: 454 (16%) received capped doses of LMWH, and the remaining 2392 received uncapped doses.					
32497167	6	71	contain	had	1033:1035	arg2	1					1046:1046	1	1046:1046	1	1046:1046	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	71	contain	had	1033:1035	arg1	122					1022:1024	122	1022:1024	122	1022:1024	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	71	contain	had	1033:1035	arg1	%					1030:1030	4.3%	1027:1030	4.3%	1027:1030	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	71	contain	had	1033:1035	arg2	outcomes					1065:1072	the composite outcomes	1051:1072	the composite outcomes	1051:1072	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	7	72	theme	Unadjusted	1075:1084	arg1	rates					1094:1098	Unadjusted outcome rates	1075:1098	Unadjusted outcome rates	1075:1098	Unadjusted outcome rates revealed that capped dosing was associated with a decrease in the composite outcome (rate ratio, 0.22; 95% confidence interval [CI], 0.04-0.75).					
32497167	0	73	from	treatment	50:58	arg1	patients					89:96	patients	89:96	patients with obesity (RIETE registry)	89:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	0	74	theme	low-molecular-weight	10:29	arg1	dosing					39:44	low-molecular-weight heparin dosing	10:44	low-molecular-weight heparin dosing	10:44	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	6	75	theme	Thirty-one	921:930	arg1	patients					932:939	Thirty-one patients	921:939	Thirty-one patients (1.9%)	921:946	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	6	75	theme	Thirty-one	921:930	arg1	%					945:945	1.9%	942:945	1.9%	942:945	Thirty-one patients (1.9%) had VTE recurrences, 38 (1.3%) had bleeding episodes, 65 (2.3%) died, and 122 (4.3%) had at least 1 of the composite outcomes.					
32497167	9	76	theme	acceptable	1513:1522	arg1	dosing					1492:1497	capped dosing	1485:1497	capped dosing of LMWH	1485:1505	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	9	76	theme	acceptable	1513:1522	arg1	alternative					1524:1534	an acceptable alternative	1510:1534	an acceptable alternative to uncapped dosing based on body weight	1510:1574	These retrospective observational data suggest that capped dosing of LMWH is an acceptable alternative to uncapped dosing based on body weight, given the significantly lower composite event rate of VTE recurrence, major bleeding, and all-cause death.					
32497167	8	77	theme	capped	1305:1310	arg1	doses					1312:1316	capped doses	1305:1316	capped doses	1305:1316	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	2	78	theme	composite	384:392	arg1	outcomes					394:401	the primary composite outcomes	372:401	the primary composite outcomes (VTE recurrence, major bleeding, or death)	372:444	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	78	theme	composite	384:392	arg1	recurrence					408:417	VTE recurrence	404:417	VTE recurrence	404:417	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	2	78	theme	composite	384:392	arg1	death					439:443	death	439:443	death	439:443	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
32497167	0	79	theme	venous	63:68	arg1	thromboembolism					70:84	venous thromboembolism	63:84	venous thromboembolism in patients with obesity (RIETE registry)	63:126	Comparing low-molecular-weight heparin dosing for treatment of venous thromboembolism in patients with obesity (RIETE registry).					
32497167	1	80	theme	venous	304:309	arg1	VTE					328:330	VTE	328:330	VTE	328:330	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	1	80	theme	venous	304:309	arg1	thromboembolism					311:325	venous thromboembolism	304:325	venous thromboembolism (VTE)	304:331	Because of the absence of comparative evidence, current guidelines and product monographs diverge in the dosing of low-molecular-weight heparin (LMWH) for obese patients with venous thromboembolism (VTE).					
32497167	3	81	theme	regression	604:613	arg1	analysis					615:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis was used to account for possible confounders.					
32497167	8	82	theme	lower	1336:1340	arg1	rates					1342:1346	significantly lower rates	1322:1346	significantly lower rates of the composite outcome	1322:1371	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	3	83	theme	Multivariable	581:593	arg1	analysis					615:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis	581:622	Multivariable logistic regression analysis was used to account for possible confounders.					
32497167	8	84	theme	0.16	1386:1389	arg1	odds					1374:1377	odds	1374:1377	odds	1374:1377	Multivariable analysis confirmed that patients who received capped doses had significantly lower rates of the composite outcome (odds ratio, 0.16; 95% CI, 0.04-0.68) while receiving LMWH.					
32497167	5	85	theme	20.6	879:882	arg1	days					901:904	14.8 (20.6) and 14.3 (32.3) days	873:904	14.8 (20.6) and 14.3 (32.3) days	873:904	Mean (standard deviation) LMWH treatment duration was 14.8 (20.6) and 14.3 (32.3) days, respectively.					
32497167	2	86	theme	RIETE	346:350	arg1	registry					352:359	the RIETE registry	342:359	the RIETE registry	342:359	We used the RIETE registry to compare the primary composite outcomes (VTE recurrence, major bleeding, or death) in patients with VTE who weighed >100 kg during LMWH therapy with capped doses of LMWH (18 000 IU/d) vs uncapped doses (>18 000 IU/d).					
33388645	8	0	theme	<	1675:1675	arg1	%					1682:1682	RSD < 0.67 %	1671:1682	RSD < 0.67 %	1671:1682	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	0	theme	<	1675:1675	arg1	stability					1660:1668	stability	1660:1668	stability (RSD < 0.67 %)	1660:1683	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	10	1	theme	tumor	2001:2005	arg1	TNF-α					2026:2030	TNF-α	2026:2030	TNF-α	2026:2030	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	1	theme	tumor	2001:2005	arg1	Factor-α					2016:2023	tumor Necrosis Factor-α	2001:2023	tumor Necrosis Factor-α (TNF-α)	2001:2031	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	8	2	with	precision	1634:1642	arg1	linearity					1541:1549	good linearity	1536:1549	good linearity (correlation coefficients > 0.9990)	1536:1585	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	2	with	precision	1634:1642	arg1	coefficients					1564:1575	correlation coefficients > 0.9990	1552:1584	correlation coefficients > 0.9990	1552:1584	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	2	with	precision	1634:1642	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	2	with	precision	1634:1642	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	9	3	located	detected	1771:1778	arg2	constituents					1749:1760	All five major constituents	1734:1760	All five major constituents	1734:1760	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	9	3	located	detected	1771:1778	arg1	fraction					1798:1805	HZE and HZE-60 fraction	1783:1805	HZE and HZE-60 fraction	1783:1805	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	3	4	theme	major	527:531	arg1	constituents					546:557	the major contribution constituents	523:557	the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect	523:614	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	9	5	from	HZE-30	1835:1840	arg1	HZE-95					1853:1858	HZE-95	1853:1858	HZE-95	1853:1858	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	11	6	theme	HZE	2146:2148	arg1	bioactivity					2131:2141	the bioactivity	2127:2141	the bioactivity of HZE	2127:2148	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	11	6	theme	HZE	2146:2148	arg1	effect					2167:2172	the syngeneic effect	2153:2172	the syngeneic effect of its constituents	2153:2192	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	1	7	from	China	275:279	arg1	treatment					228:236	treatment	228:236	treatment of various inflammatory disorders in China	228:279	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	8	8	theme	%	1707:1707	arg1	%					1716:1716	99.52 %-101.23 %	1701:1716	99.52 %-101.23 %	1701:1716	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	8	theme	%	1707:1707	arg1	recoveries					1689:1698	recoveries	1689:1698	recoveries (99.52 %-101.23 %, RSD < 0.91 %)	1689:1731	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	1	9	used	used	219:222	arg2	rhizome					167:173	rhizome	167:173	rhizome	167:173	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	1	9	used	used	219:222	arg2	root					158:161	root	158:161	root	158:161	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	1	10	from	disorders	262:270	arg1	China					275:279	China	275:279	China	275:279	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	3	11	theme	Hu-Zhang	562:569	arg1	constituents					546:557	the major contribution constituents	523:557	the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect	523:614	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	8	12	theme	constituents	1364:1375	arg1	contents					1341:1348	The contents	1337:1348	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31))	1337:1486	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	13	theme	RSD	1719:1721	arg1	%					1730:1730	RSD < 0.91 %	1719:1730	RSD < 0.91 %	1719:1730	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	5	14	theme	major	983:987	arg1	constituents					989:1000	five major constituents	978:1000	five major constituents	978:1000	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	10	15	theme	monocyte	2059:2066	arg1	MCP-1					2091:2095	MCP-1	2091:2095	MCP-1	2091:2095	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	15	theme	monocyte	2059:2066	arg1	protein-1					2080:2088	monocyte chemotactic protein-1	2059:2088	monocyte chemotactic protein-1 (MCP-1)	2059:2096	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	3	16	theme	anti-inflammatory	591:607	arg1	effect					609:614	its anti-inflammatory effect	587:614	its anti-inflammatory effect	587:614	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	11	17	theme	Hu-Zhang	2280:2287	arg1	effect					2270:2275	the anti-inflammatory effect	2248:2275	the anti-inflammatory effect of Hu-Zhang	2248:2287	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	1	18	theme	cuspidatum	188:197	arg1	root					158:161	root	158:161	root	158:161	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	1	18	theme	cuspidatum	188:197	arg1	rhizome					167:173	rhizome	167:173	rhizome	167:173	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	1	19	dep	root	158:161	arg1	The					154:156	The	154:156	The	154:156	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	10	20	theme	significant	1894:1904	arg1	activity					1924:1931	significant anti-inflammatory activity	1894:1931	significant anti-inflammatory activity	1894:1931	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	5	21	theme	anti-inflammatory	1019:1035	arg1	fractions					1046:1054	three anti-inflammatory activity fractions	1013:1054	three anti-inflammatory activity fractions	1013:1054	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	3	22	dep	understand	512:521	arg1	to					509:510	to	509:510	to	509:510	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	2	23	theme	anti-inflammatory	472:488	arg1	activity					490:497	the most potent anti-inflammatory activity	456:497	the most potent anti-inflammatory activity	456:497	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	8	24	theme	good	1536:1539	arg1	linearity					1541:1549	good linearity	1536:1549	good linearity (correlation coefficients > 0.9990)	1536:1585	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	24	theme	good	1536:1539	arg1	coefficients					1564:1575	correlation coefficients > 0.9990	1552:1584	correlation coefficients > 0.9990	1552:1584	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	12	25	theme	action	2452:2457	arg1	control					2423:2429	its quality control	2411:2429	its quality control	2411:2429	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	12	25	theme	action	2452:2457	arg1	mechanism					2439:2447	the mechanism	2435:2447	the mechanism of action	2435:2457	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	4	26	theme	time-of-flight	814:827	arg1	UPLC-Q-TOF/MS					848:860	UPLC-Q-TOF/MS	848:860	UPLC-Q-TOF/MS	848:860	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	4	26	theme	time-of-flight	814:827	arg1	spectrometry					834:845	quadrupole time-of-flight mass spectrometry	803:845	quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	803:870	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	0	27	theme	RAW264.7	132:139	arg1	macrophages					141:151	UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages	82:151	UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages	82:151	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	12	28	theme	quality	2415:2421	arg1	control					2423:2429	its quality control	2411:2429	its quality control	2411:2429	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	4	29	theme	ultra-performance	750:766	arg1	chromatography					775:788	an ultra-performance liquid chromatography	747:788	an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	747:870	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	0	30	theme	Anti-inflammatory	0:16	arg1	constituents					18:29	Anti-inflammatory constituents	0:29	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.	0:152	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	5	31	from	difference	964:973	arg1	HZE					1005:1007	HZE	1005:1007	HZE	1005:1007	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	5	31	from	difference	964:973	arg1	fractions					1046:1054	three anti-inflammatory activity fractions	1013:1054	three anti-inflammatory activity fractions	1013:1054	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	2	32	theme	all	406:408	arg1	extract					380:386	the ethanol extract	368:386	the ethanol extract of Hu-Zhang (HZE) all	368:408	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	2	33	theme	HZE	401:403	arg1	all					406:408	Hu-Zhang (HZE) all	391:408	Hu-Zhang (HZE) all	391:408	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	12	34	theme	chemical	2332:2339	arg1	constituents					2341:2352	the anti-inflammatory chemical constituents	2310:2352	the anti-inflammatory chemical constituents	2310:2352	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	2	35	theme	Hu-Zhang	391:398	arg1	all					406:408	Hu-Zhang (HZE) all	391:408	Hu-Zhang (HZE) all	391:408	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	7	36	theme	compounds	1226:1234	arg1	total					1214:1218	a total	1212:1218	a total of 31 compounds	1212:1234	The results showed that a total of 31 compounds were identified from HZE-60, including 12 anthraquinones, 7 diphenylethenes, 9 phenols and 3 others.					
33388645	6	37	theme	major	1109:1113	arg1	constituents					1115:1126	major constituents	1109:1126	major constituents	1109:1126	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	3	38	theme	composition-activity	630:649	arg1	method					664:669	quantitative composition-activity relationship method	617:669	quantitative composition-activity relationship method	617:669	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	0	39	dep	root	38:41	arg1	the					34:36	the	34:36	the	34:36	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	2	40	from	extract	380:386	arg1	fractions					325:333	three fractions	319:333	three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all	319:408	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	2	40	from	extract	380:386	arg1	HZE-95					355:360	HZE-95	355:360	HZE-95	355:360	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	2	40	from	extract	380:386	arg1	HZE-60					344:349	HZE-60	344:349	HZE-60	344:349	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	10	41	theme	pro-inflammatory	1961:1976	arg1	NO					1997:1998	NO	1997:1998	NO	1997:1998	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	41	theme	pro-inflammatory	1961:1976	arg1	Factor-α					2016:2023	tumor Necrosis Factor-α	2001:2023	tumor Necrosis Factor-α (TNF-α)	2001:2031	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	41	theme	pro-inflammatory	1961:1976	arg1	protein-1					2080:2088	monocyte chemotactic protein-1	2059:2088	monocyte chemotactic protein-1 (MCP-1)	2059:2096	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	41	theme	pro-inflammatory	1961:1976	arg1	mediators					1978:1986	supernatant pro-inflammatory mediators	1949:1986	supernatant pro-inflammatory mediators	1949:1986	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	41	theme	pro-inflammatory	1961:1976	arg1	interleukin-6					2034:2046	interleukin-6	2034:2046	interleukin-6 (IL-6)	2034:2053	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	4	42	theme	spectrometry	834:845	arg1	approach					863:870	quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	803:870	quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	803:870	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	8	43	theme	RSD	1645:1647	arg1	%					1656:1656	RSD < 0.01 %	1645:1656	RSD < 0.01 %	1645:1656	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	43	theme	RSD	1645:1647	arg1	precision					1634:1642	precision	1634:1642	precision (RSD < 0.01 %)	1634:1657	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	11	44	theme	syngeneic	2157:2165	arg1	bioactivity					2131:2141	the bioactivity	2127:2141	the bioactivity of HZE	2127:2148	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	11	44	theme	syngeneic	2157:2165	arg1	effect					2167:2172	the syngeneic effect	2153:2172	the syngeneic effect of its constituents	2153:2192	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	8	45	theme	RSD	1619:1621	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	45	theme	RSD	1619:1621	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	1	46	theme	inflammatory	249:260	arg1	disorders					262:270	various inflammatory disorders	241:270	various inflammatory disorders in China	241:279	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	8	47	dep	%	1716:1716	arg1	%					1730:1730	RSD < 0.91 %	1719:1730	RSD < 0.91 %	1719:1730	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	4	48	from	constituents	699:710	arg1	HZE-60					715:720	HZE-60	715:720	HZE-60	715:720	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	6	49	theme	RAW264.7	1166:1173	arg1	macrophages					1175:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	11	50	theme	constituents	2181:2192	arg1	bioactivity					2131:2141	the bioactivity	2127:2141	the bioactivity of HZE	2127:2148	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	11	50	theme	constituents	2181:2192	arg1	effect					2167:2172	the syngeneic effect	2153:2172	the syngeneic effect of its constituents	2153:2192	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	10	51	theme	Necrosis	2007:2014	arg1	TNF-α					2026:2030	TNF-α	2026:2030	TNF-α	2026:2030	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	51	theme	Necrosis	2007:2014	arg1	Factor-α					2016:2023	tumor Necrosis Factor-α	2001:2023	tumor Necrosis Factor-α (TNF-α)	2001:2031	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	2	52	theme	pervious	289:296	arg1	study					298:302	our pervious study	285:302	our pervious study	285:302	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	8	53	theme	RSD	1671:1673	arg1	%					1682:1682	RSD < 0.67 %	1671:1682	RSD < 0.67 %	1671:1682	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	53	theme	RSD	1671:1673	arg1	stability					1660:1668	stability	1660:1668	stability (RSD < 0.67 %)	1660:1683	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	5	54	theme	major	908:912	arg1	constituents					914:925	five major constituents	903:925	five major constituents identified in HZE-60	903:946	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	12	55	theme	Hu-Zhang	2357:2364	arg1	constituents					2341:2352	the anti-inflammatory chemical constituents	2310:2352	the anti-inflammatory chemical constituents	2310:2352	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	3	56	theme	contribution	533:544	arg1	constituents					546:557	the major contribution constituents	523:557	the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect	523:614	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	12	57	theme	anti-inflammatory	2314:2330	arg1	constituents					2341:2352	the anti-inflammatory chemical constituents	2310:2352	the anti-inflammatory chemical constituents	2310:2352	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	3	58	theme	responsible	571:581	arg1	constituents					546:557	the major contribution constituents	523:557	the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect	523:614	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	11	59	theme	anti-inflammatory	2252:2268	arg1	effect					2270:2275	the anti-inflammatory effect	2248:2275	the anti-inflammatory effect of Hu-Zhang	2248:2287	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	1	60	from	treatment	228:236	arg1	China					275:279	China	275:279	China	275:279	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	10	61	theme	chemotactic	2068:2078	arg1	MCP-1					2091:2095	MCP-1	2091:2095	MCP-1	2091:2095	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	61	theme	chemotactic	2068:2078	arg1	protein-1					2080:2088	monocyte chemotactic protein-1	2059:2088	monocyte chemotactic protein-1 (MCP-1)	2059:2096	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	8	62	theme	major	1358:1362	arg1	polydatin					1378:1386	polydatin (6)	1378:1390	polydatin (6)	1378:1390	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	62	theme	major	1358:1362	arg1	emodin					1475:1480	emodin (31)	1475:1485	emodin (31)	1475:1485	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	62	theme	major	1358:1362	arg1	emodin-8-O-β-d-glucoside					1441:1464	emodin-8-O-β-d-glucoside (21)	1441:1469	emodin-8-O-β-d-glucoside (21)	1441:1469	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	62	theme	major	1358:1362	arg1	emodin-1-O-β-d-glucoside					1410:1433	emodin-1-O-β-d-glucoside (15)	1410:1438	emodin-1-O-β-d-glucoside (15)	1410:1438	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	62	theme	major	1358:1362	arg1	constituents					1364:1375	five major constituents	1353:1375	five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31))	1353:1486	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	62	theme	major	1358:1362	arg1	resveratrol					1393:1403	resveratrol (7)	1393:1407	resveratrol (7)	1393:1407	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	0	63	theme	cuspidatum	68:77	arg1	rhizome					47:53	rhizome	47:53	rhizome	47:53	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	0	63	theme	cuspidatum	68:77	arg1	root					38:41	root	38:41	root	38:41	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	12	64	theme	further	2386:2392	arg1	investigation					2394:2406	further investigation	2386:2406	further investigation	2386:2406	The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.					
33388645	6	65	theme	anti-inflammatory	1080:1096	arg1	effects					1098:1104	the anti-inflammatory effects	1076:1104	the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1076:1185	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	0	66	theme	UPLC-PDA-QTOF/MS	82:97	arg1	macrophages					141:151	UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages	82:151	UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages	82:151	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	8	67	theme	<	1723:1723	arg1	%					1730:1730	RSD < 0.91 %	1719:1730	RSD < 0.91 %	1719:1730	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	0	68	from	constituents	18:29	arg1	rhizome					47:53	rhizome	47:53	rhizome	47:53	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	0	68	from	constituents	18:29	arg1	root					38:41	root	38:41	root	38:41	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	10	69	theme	anti-inflammatory	1906:1922	arg1	activity					1924:1931	significant anti-inflammatory activity	1894:1931	significant anti-inflammatory activity	1894:1931	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	0	70	theme	lipopolysaccharide-activated	103:130	arg1	macrophages					141:151	UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages	82:151	UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages	82:151	Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.					
33388645	6	71	from	effects	1098:1104	arg1	macrophages					1175:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	9	72	located	detected	1823:1830	arg1	HZE-30					1835:1840	HZE-30	1835:1840	HZE-30	1835:1840	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	9	72	located	detected	1823:1830	arg1	31					1847:1848	31	1847:1848	31	1847:1848	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	9	72	located	detected	1823:1830	arg2	6					1817:1817	6	1817:1817	6	1817:1817	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	5	73	theme	activity	1037:1044	arg1	fractions					1046:1054	three anti-inflammatory activity fractions	1013:1054	three anti-inflammatory activity fractions	1013:1054	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	6	74	theme	-activated	1155:1164	arg1	macrophages					1175:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	2	75	theme	potent	465:470	arg1	activity					490:497	the most potent anti-inflammatory activity	456:497	the most potent anti-inflammatory activity	456:497	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	8	76	theme	correlation	1552:1562	arg1	linearity					1541:1549	good linearity	1536:1549	good linearity (correlation coefficients > 0.9990)	1536:1585	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	76	theme	correlation	1552:1562	arg1	coefficients					1564:1575	correlation coefficients > 0.9990	1552:1584	correlation coefficients > 0.9990	1552:1584	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	77	with	recoveries	1689:1698	arg1	linearity					1541:1549	good linearity	1536:1549	good linearity (correlation coefficients > 0.9990)	1536:1585	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	77	with	recoveries	1689:1698	arg1	coefficients					1564:1575	correlation coefficients > 0.9990	1552:1584	correlation coefficients > 0.9990	1552:1584	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	77	with	recoveries	1689:1698	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	77	with	recoveries	1689:1698	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	11	78	theme	great	2224:2228	arg1	contributions					2230:2242	great contributions	2224:2242	great contributions	2224:2242	Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang.					
33388645	4	79	theme	quadrupole	803:812	arg1	UPLC-Q-TOF/MS					848:860	UPLC-Q-TOF/MS	848:860	UPLC-Q-TOF/MS	848:860	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	4	79	theme	quadrupole	803:812	arg1	spectrometry					834:845	quadrupole time-of-flight mass spectrometry	803:845	quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	803:870	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	2	80	theme	ethanol	372:378	arg1	extract					380:386	the ethanol extract	368:386	the ethanol extract of Hu-Zhang (HZE) all	368:408	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	5	81	theme	constituents	989:1000	arg1	difference					964:973	the difference	960:973	the difference of five major constituents in HZE and three anti-inflammatory activity fractions	960:1054	Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions.					
33388645	2	82	dep	fractions	325:333	arg1	fractions					325:333	three fractions	319:333	three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all	319:408	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	2	82	dep	fractions	325:333	arg1	HZE-95					355:360	HZE-95	355:360	HZE-95	355:360	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	2	82	dep	fractions	325:333	arg1	HZE-60					344:349	HZE-60	344:349	HZE-60	344:349	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	6	83	theme	lipopolysaccharide	1131:1148	arg1	macrophages					1175:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1131:1185	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	3	84	theme	relationship	651:662	arg1	method					664:669	quantitative composition-activity relationship method	617:669	quantitative composition-activity relationship method	617:669	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	4	85	theme	liquid	768:773	arg1	chromatography					775:788	an ultra-performance liquid chromatography	747:788	an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	747:870	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	6	86	theme	constituents	1115:1126	arg1	effects					1098:1104	the anti-inflammatory effects	1076:1104	the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages	1076:1185	Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages.					
33388645	9	87	theme	major	1743:1747	arg1	constituents					1749:1760	All five major constituents	1734:1760	All five major constituents	1734:1760	All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95.					
33388645	8	88	theme	satisfactory	1591:1602	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	88	theme	satisfactory	1591:1602	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	3	89	theme	quantitative	617:628	arg1	method					664:669	quantitative composition-activity relationship method	617:669	quantitative composition-activity relationship method	617:669	In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed.					
33388645	2	90	theme	NO	424:425	arg1	production					427:436	NO production	424:436	NO production	424:436	In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity.					
33388645	8	91	with	stability	1660:1668	arg1	linearity					1541:1549	good linearity	1536:1549	good linearity (correlation coefficients > 0.9990)	1536:1585	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	91	with	stability	1660:1668	arg1	coefficients					1564:1575	correlation coefficients > 0.9990	1552:1584	correlation coefficients > 0.9990	1552:1584	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	91	with	stability	1660:1668	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	91	with	stability	1660:1668	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	92	theme	<	1649:1649	arg1	%					1656:1656	RSD < 0.01 %	1645:1656	RSD < 0.01 %	1645:1656	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	92	theme	<	1649:1649	arg1	precision					1634:1642	precision	1634:1642	precision (RSD < 0.01 %)	1634:1657	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	93	dep	constituents	1364:1375	arg1	polydatin					1378:1386	polydatin (6)	1378:1390	polydatin (6)	1378:1390	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	93	dep	constituents	1364:1375	arg1	emodin					1475:1480	emodin (31)	1475:1485	emodin (31)	1475:1485	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	93	dep	constituents	1364:1375	arg1	emodin-8-O-β-d-glucoside					1441:1464	emodin-8-O-β-d-glucoside (21)	1441:1469	emodin-8-O-β-d-glucoside (21)	1441:1469	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	93	dep	constituents	1364:1375	arg1	emodin-1-O-β-d-glucoside					1410:1433	emodin-1-O-β-d-glucoside (15)	1410:1438	emodin-1-O-β-d-glucoside (15)	1410:1438	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	93	dep	constituents	1364:1375	arg1	constituents					1364:1375	five major constituents	1353:1375	five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31))	1353:1486	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	93	dep	constituents	1364:1375	arg1	resveratrol					1393:1403	resveratrol (7)	1393:1407	resveratrol (7)	1393:1407	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	10	94	theme	supernatant	1949:1959	arg1	NO					1997:1998	NO	1997:1998	NO	1997:1998	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	94	theme	supernatant	1949:1959	arg1	Factor-α					2016:2023	tumor Necrosis Factor-α	2001:2023	tumor Necrosis Factor-α (TNF-α)	2001:2031	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	94	theme	supernatant	1949:1959	arg1	protein-1					2080:2088	monocyte chemotactic protein-1	2059:2088	monocyte chemotactic protein-1 (MCP-1)	2059:2096	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	94	theme	supernatant	1949:1959	arg1	mediators					1978:1986	supernatant pro-inflammatory mediators	1949:1986	supernatant pro-inflammatory mediators	1949:1986	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	10	94	theme	supernatant	1949:1959	arg1	interleukin-6					2034:2046	interleukin-6	2034:2046	interleukin-6 (IL-6)	2034:2053	Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1).					
33388645	8	95	theme	<	1623:1623	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	95	theme	<	1623:1623	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	4	96	theme	mass	829:832	arg1	UPLC-Q-TOF/MS					848:860	UPLC-Q-TOF/MS	848:860	UPLC-Q-TOF/MS	848:860	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	4	96	theme	mass	829:832	arg1	spectrometry					834:845	quadrupole time-of-flight mass spectrometry	803:845	quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach	803:870	Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach.					
33388645	1	97	theme	various	241:247	arg1	disorders					262:270	various inflammatory disorders	241:270	various inflammatory disorders in China	241:279	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
33388645	8	98	with	UPLC-PDA	1522:1529	arg1	linearity					1541:1549	good linearity	1536:1549	good linearity (correlation coefficients > 0.9990)	1536:1585	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	98	with	UPLC-PDA	1522:1529	arg1	coefficients					1564:1575	correlation coefficients > 0.9990	1552:1584	correlation coefficients > 0.9990	1552:1584	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	98	with	UPLC-PDA	1522:1529	arg1	%					1630:1630	RSD < 0.99 %	1619:1630	RSD < 0.99 %	1619:1630	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	8	98	with	UPLC-PDA	1522:1529	arg1	repeatability					1604:1616	satisfactory repeatability	1591:1616	satisfactory repeatability (RSD < 0.99 %)	1591:1631	The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %).					
33388645	1	99	theme	disorders	262:270	arg1	treatment					228:236	treatment	228:236	treatment of various inflammatory disorders in China	228:279	The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China.					
34116093	1	0	with	modification	161:172	arg1	anhydride					209:217	stearic anhydride	201:217	stearic anhydride through microwave action with 4-dimethylaminopyridine	201:271	This work investigated the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine as catalyst.					
34116093	4	1	with	film	805:808	arg1	PC0					842:844	PC0	842:844	PC0	842:844	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	4	1	with	film	805:808	arg1	pectin					834:839	native low-methoxy pectin	815:839	native low-methoxy pectin (PC0)	815:845	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	0	2	theme	hydrophobic	89:99	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	5	3	theme	composite	1117:1125	arg1	films					1127:1131	the composite films	1113:1131	the composite films	1113:1131	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	4	4	theme	modified	863:870	arg1	pectins					872:878	modified pectins	863:878	modified pectins	863:878	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	2	5	dep	Fourier	286:292	arg1	transform					294:302	transform	294:302	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses	294:383	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	4	6	theme	contact	774:780	arg1	angle					782:786	the contact angle	770:786	the contact angle of 65.3° for the film with native low-methoxy pectin (PC0)	770:845	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	4	7	theme	Composite	675:683	arg1	films					685:689	Composite films	675:689	Composite films	675:689	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	0	8	theme	edible	82:87	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	0	9	from	characterization	45:60	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	2	10	theme	%	530:530	arg1	SGR					518:520	SGR	518:520	SGR	518:520	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	10	theme	%	530:530	arg1	ratio					511:515	the maximum stearic acid grafting ratio	477:515	the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin	477:554	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	1	11	theme	low-methoxy	177:187	arg1	pectin					189:194	low-methoxy pectin	177:194	low-methoxy pectin	177:194	This work investigated the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine as catalyst.					
34116093	0	12	theme	pectin/chitosan	101:115	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	0	13	from	Preparation	32:42	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	1	14	theme	pectin	189:194	arg1	modification					161:172	the modification	157:172	the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine	157:271	This work investigated the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine as catalyst.					
34116093	2	15	theme	maximum	481:487	arg1	SGR					518:520	SGR	518:520	SGR	518:520	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	15	theme	maximum	481:487	arg1	ratio					511:515	the maximum stearic acid grafting ratio	477:515	the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin	477:554	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	5	16	theme	at	1101:1102	arg1	break					1104:1108	elongation at break	1090:1108	elongation at break	1090:1108	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	3	17	theme	stearic	577:583	arg1	acid					585:588	stearic acid	577:588	stearic acid	577:588	The introduction of stearic acid was shown to significantly improve the emulsifying activity and stability of pectin.					
34116093	0	18	theme	Stearic	0:6	arg1	acid					8:11	Stearic acid	0:11	Stearic acid esterified pectin	0:29	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	2	19	theme	photoelectron	343:355	arg1	XPS					371:373	XPS	371:373	XPS	371:373	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	19	theme	photoelectron	343:355	arg1	spectroscopy					357:368	X-ray photoelectron spectroscopy	337:368	X-ray photoelectron spectroscopy (XPS)	337:374	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	1	20	theme	stearic	201:207	arg1	anhydride					209:217	stearic anhydride	201:217	stearic anhydride through microwave action with 4-dimethylaminopyridine	201:271	This work investigated the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine as catalyst.					
34116093	5	21	theme	anchoring	1145:1153	arg1	"					1161:1161	the "anchoring effect"	1140:1161	the "anchoring effect"	1140:1161	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	5	22	theme	appropriate	1012:1022	arg1	amount					1024:1029	an appropriate amount	1009:1029	an appropriate amount of aliphatic chains	1009:1049	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	5	22	theme	appropriate	1012:1022	arg1	chains					1044:1049	aliphatic chains	1034:1049	aliphatic chains	1034:1049	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	2	23	theme	X-ray	337:341	arg1	XPS					371:373	XPS	371:373	XPS	371:373	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	23	theme	X-ray	337:341	arg1	spectroscopy					357:368	X-ray photoelectron spectroscopy	337:368	X-ray photoelectron spectroscopy (XPS)	337:374	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	4	24	theme	native	815:820	arg1	PC0					842:844	PC0	842:844	PC0	842:844	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	4	24	theme	native	815:820	arg1	pectin					834:839	native low-methoxy pectin	815:839	native low-methoxy pectin (PC0)	815:845	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	3	25	theme	acid	585:588	arg1	introduction					561:572	The introduction	557:572	The introduction of stearic acid	557:588	The introduction of stearic acid was shown to significantly improve the emulsifying activity and stability of pectin.					
34116093	5	26	theme	effect	1155:1160	arg1	"					1161:1161	the "anchoring effect"	1140:1161	the "anchoring effect"	1140:1161	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	4	27	theme	angle	901:905	arg1	increase					907:914	a significant angle increase	887:914	a significant angle increase	887:914	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	2	28	theme	esterification	447:460	arg1	reaction					462:469	esterification reaction	447:469	esterification reaction	447:469	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	4	29	theme	hydrophobic	978:988	arg1	surface					990:996	a hydrophobic surface	976:996	a hydrophobic surface	976:996	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	3	30	theme	pectin	667:672	arg1	stability					654:662	stability	654:662	stability	654:662	The introduction of stearic acid was shown to significantly improve the emulsifying activity and stability of pectin.					
34116093	3	30	theme	pectin	667:672	arg1	activity					641:648	the emulsifying activity	625:648	the emulsifying activity	625:648	The introduction of stearic acid was shown to significantly improve the emulsifying activity and stability of pectin.					
34116093	1	31	with	anhydride	209:217	arg1	4-dimethylaminopyridine					249:271	4-dimethylaminopyridine	249:271	4-dimethylaminopyridine	249:271	This work investigated the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine as catalyst.					
34116093	0	32	from	application	67:77	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	0	33	theme	composite	117:125	arg1	films					127:131	edible hydrophobic pectin/chitosan composite films	82:131	edible hydrophobic pectin/chitosan composite films	82:131	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	1	34	theme	microwave	227:235	arg1	action					237:242	microwave action	227:242	microwave action	227:242	This work investigated the modification of low-methoxy pectin with stearic anhydride through microwave action with 4-dimethylaminopyridine as catalyst.					
34116093	2	35	theme	grafting	502:509	arg1	SGR					518:520	SGR	518:520	SGR	518:520	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	35	theme	grafting	502:509	arg1	ratio					511:515	the maximum stearic acid grafting ratio	477:515	the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin	477:554	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	5	36	theme	films	1127:1131	arg1	strength					1077:1084	tensile strength	1069:1084	tensile strength	1069:1084	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	5	36	theme	films	1127:1131	arg1	break					1104:1108	elongation at break	1090:1108	elongation at break	1090:1108	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	2	37	theme	modified	540:547	arg1	pectin					549:554	the modified pectin	536:554	the modified pectin	536:554	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	4	38	theme	contact	934:940	arg1	angle					942:946	the highest contact angle	922:946	the highest contact angle reaching 101.9°	922:962	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	2	39	theme	acid	497:500	arg1	SGR					518:520	SGR	518:520	SGR	518:520	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	39	theme	acid	497:500	arg1	ratio					511:515	the maximum stearic acid grafting ratio	477:515	the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin	477:554	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	4	40	theme	highest	926:932	arg1	angle					942:946	the highest contact angle	922:946	the highest contact angle reaching 101.9°	922:962	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	5	41	theme	tensile	1069:1075	arg1	strength					1077:1084	tensile strength	1069:1084	tensile strength	1069:1084	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	0	42	dep	Preparation	32:42	arg1	acid					8:11	Stearic acid	0:11	Stearic acid esterified pectin	0:29	Stearic acid esterified pectin: Preparation, characterization, and application in edible hydrophobic pectin/chitosan composite films.					
34116093	5	43	theme	elongation	1090:1099	arg1	break					1104:1108	elongation at break	1090:1108	elongation at break	1090:1108	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	2	44	theme	stearic	489:495	arg1	SGR					518:520	SGR	518:520	SGR	518:520	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	44	theme	stearic	489:495	arg1	ratio					511:515	the maximum stearic acid grafting ratio	477:515	the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin	477:554	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	5	45	dep	strength	1077:1084	arg1	the					1065:1067	the	1065:1067	the	1065:1067	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	4	46	theme	low-methoxy	822:832	arg1	PC0					842:844	PC0	842:844	PC0	842:844	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	4	46	theme	low-methoxy	822:832	arg1	pectin					834:839	native low-methoxy pectin	815:839	native low-methoxy pectin (PC0)	815:845	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	4	47	theme	modified	721:728	arg1	pectins					730:736	the modified pectins	717:736	the modified pectins	717:736	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	4	48	theme	significant	889:899	arg1	increase					907:914	a significant angle increase	887:914	a significant angle increase	887:914	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	3	49	theme	emulsifying	629:639	arg1	activity					641:648	the emulsifying activity	625:648	the emulsifying activity	625:648	The introduction of stearic acid was shown to significantly improve the emulsifying activity and stability of pectin.					
34116093	2	50	theme	infrared	304:311	arg1	FTIR					327:330	FTIR	327:330	FTIR	327:330	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	2	50	theme	infrared	304:311	arg1	spectroscopy					313:324	infrared spectroscopy	304:324	infrared spectroscopy (FTIR)	304:331	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	5	51	theme	aliphatic	1034:1042	arg1	chains					1044:1049	aliphatic chains	1034:1049	aliphatic chains	1034:1049	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	4	52	theme	65.3°	791:795	arg1	angle					782:786	the contact angle	770:786	the contact angle of 65.3° for the film with native low-methoxy pectin (PC0)	770:845	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	2	53	dep	spectroscopy	313:324	arg1	analyses					376:383	analyses	376:383	analyses	376:383	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
34116093	4	54	with	films	852:856	arg1	pectins					872:878	modified pectins	863:878	modified pectins	863:878	Composite films were prepared by blending the modified pectins and chitosan, and compared with the contact angle of 65.3° for the film with native low-methoxy pectin (PC0), the films with modified pectins showed a significant angle increase, with the highest contact angle reaching 101.9°, indicating a hydrophobic surface.					
34116093	5	55	theme	chains	1044:1049	arg1	amount					1024:1029	an appropriate amount	1009:1029	an appropriate amount of aliphatic chains	1009:1049	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	5	55	theme	chains	1044:1049	arg1	chains					1044:1049	aliphatic chains	1034:1049	aliphatic chains	1034:1049	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	5	56	from	break	1104:1108	arg1	elongation					1090:1099	elongation at break	1090:1108	elongation at break	1090:1108	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	5	56	from	break	1104:1108	arg1	at					1101:1102	elongation at break	1090:1108	elongation at break	1090:1108	Moreover, an appropriate amount of aliphatic chains could improve the tensile strength and elongation at break of the composite films due to the "anchoring effect".					
34116093	2	57	theme	stearic	400:406	arg1	acid					408:411	stearic acid	400:411	stearic acid	400:411	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses indicated that stearic acid was grafted on the pectin through esterification reaction, with the maximum stearic acid grafting ratio (SGR) of 10.7% for the modified pectin.					
32057874	9	0	theme	other	1242:1246	arg1	hand					1248:1251	the other hand	1238:1251	the other hand	1238:1251	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	7	1	theme	free	956:959	arg1	content					972:978	free sulfhydryl content	956:978	free sulfhydryl content	956:978	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	9	2	theme	stronger	1332:1339	arg1	network					1341:1347	this stronger network	1327:1347	this stronger network	1327:1347	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	0	3	theme	noodles	98:104	arg1	rheology					18:25	dough rheology	12:25	dough rheology	12:25	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	0	3	theme	noodles	98:104	arg1	characteristics					42:56	structural characteristics	31:56	structural characteristics	31:56	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	4	4	theme	decreased	524:532	arg1	trend					534:538	a decreased trend	522:538	a decreased trend at higher level of gum addition (0.8%)	522:577	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	9	5	theme	deformation	1378:1388	arg1	signs					1362:1366	visible signs	1354:1366	visible signs of starch deformation	1354:1388	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	7	6	theme	noodle	842:847	arg1	samples					849:855	cooked noodle samples	835:855	cooked noodle samples	835:855	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	9	7	from	%	1298:1298	arg1	levels					1265:1270	the higher levels	1254:1270	the higher levels of gum substitution at 0.5% and 0.8%	1254:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	9	7	from	%	1298:1298	arg1	substitution					1279:1290	gum substitution	1275:1290	gum substitution at 0.5% and 0.8%	1275:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	9	8	theme	network	1494:1500	arg1	lack					1479:1482	the lack	1475:1482	the lack of gluten network	1475:1500	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	2	9	dep	Krasch	217:222	arg1	effect					182:187	The effect	178:187	The effect of Artemisia sphaerocephala	178:215	The effect of Artemisia sphaerocephala Krasch.					
32057874	0	10	theme	different	111:119	arg1	levels					121:126	different levels	111:126	different levels of Artemisia sphaerocephala Krasch	111:161	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	4	11	theme	dough	496:500	arg1	system					502:507	the composite dough system	482:507	the composite dough system	482:507	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	9	12	theme	gum	1275:1277	arg1	substitution					1279:1290	gum substitution	1275:1290	gum substitution at 0.5% and 0.8%	1275:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	4	13	from	increase	439:446	arg1	properties					468:477	the viscoelastic properties	451:477	the viscoelastic properties of the composite dough system	451:507	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	9	14	theme	starch	1428:1433	arg1	molecules					1435:1443	starch molecules	1428:1443	starch molecules	1428:1443	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	5	15	theme	X-ray	709:713	arg1	diffractometer					715:728	X-ray diffractometer	709:728	X-ray diffractometer for dough samples	709:746	This trend was in agreement with the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples.					
32057874	4	16	theme	composite	486:494	arg1	system					502:507	the composite dough system	482:507	the composite dough system	482:507	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	7	17	theme	gum	1054:1056	arg1	addition					1058:1065	gum addition	1054:1065	gum addition	1054:1065	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	7	18	theme	cooked	835:840	arg1	samples					849:855	cooked noodle samples	835:855	cooked noodle samples	835:855	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	9	19	theme	visible	1354:1360	arg1	signs					1362:1366	visible signs	1354:1366	visible signs of starch deformation	1354:1388	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	3	20	theme	structural	274:283	arg1	characteristics					285:299	structural characteristics	274:299	structural characteristics	274:299	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	6	21	theme	scanning	768:775	arg1	CLSM					790:793	CLSM	790:793	CLSM	790:793	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	6	21	theme	scanning	768:775	arg1	micrographs					777:787	The confocal laser scanning micrographs	749:787	The confocal laser scanning micrographs (CLSM)	749:794	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	0	22	theme	dough	12:16	arg1	rheology					18:25	dough rheology	12:25	dough rheology	12:25	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	4	23	theme	system	502:507	arg1	properties					468:477	the viscoelastic properties	451:477	the viscoelastic properties of the composite dough system	451:507	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	3	24	theme	dough	252:256	arg1	performance					258:268	dough performance	252:268	dough performance	252:268	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	6	25	theme	laser	762:766	arg1	CLSM					790:793	CLSM	790:793	CLSM	790:793	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	6	25	theme	laser	762:766	arg1	micrographs					777:787	The confocal laser scanning micrographs	749:787	The confocal laser scanning micrographs (CLSM)	749:794	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	7	26	from	variations	862:871	arg1	properties					884:893	pasting properties	876:893	pasting properties	876:893	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	9	27	theme	higher	1258:1263	arg1	levels					1265:1270	the higher levels	1254:1270	the higher levels of gum substitution at 0.5% and 0.8%	1254:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	8	28	theme	%	1218:1218	arg1	addition					1225:1232	0.3% ASKG addition	1215:1232	0.3% ASKG addition	1215:1232	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	4	29	theme	gum	559:561	arg1	addition					563:570	gum addition	559:570	gum addition (0.8%)	559:577	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	4	29	theme	gum	559:561	arg1	%					576:576	0.8%	573:576	0.8%	573:576	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	9	30	theme	lack	1479:1482	arg1	result					1465:1470	a result	1463:1470	a result of the lack of gluten network	1463:1500	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	6	31	theme	confocal	753:760	arg1	CLSM					790:793	CLSM	790:793	CLSM	790:793	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	6	31	theme	confocal	753:760	arg1	micrographs					777:787	The confocal laser scanning micrographs	749:787	The confocal laser scanning micrographs (CLSM)	749:794	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	4	32	theme	viscoelastic	455:466	arg1	properties					468:477	the viscoelastic properties	451:477	the viscoelastic properties of the composite dough system	451:507	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	9	33	theme	substitution	1279:1290	arg1	levels					1265:1270	the higher levels	1254:1270	the higher levels of gum substitution at 0.5% and 0.8%	1254:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	0	34	theme	Krasch	156:161	arg1	levels					121:126	different levels	111:126	different levels of Artemisia sphaerocephala Krasch	111:161	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	3	35	theme	gum	225:227	arg1	addition					236:243	gum (ASKG) addition	225:243	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product	225:351	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	1	36	dep	addition	168:175	arg1	gum					164:166	gum	164:166	gum	164:166	gum addition.					
32057874	3	37	theme	wheat-chickpea	308:321	arg1	product					345:351	the wheat-chickpea composite flour-based product	304:351	the wheat-chickpea composite flour-based product	304:351	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	7	38	theme	compact	1010:1016	arg1	structure					1018:1026	a relatively more compact structure	992:1026	a relatively more compact structure was formed at the 0.3% of gum addition	992:1065	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	8	39	theme	CLSM	1106:1109	arg1	observations					1111:1122	The scanning electron microscopic and CLSM observations	1068:1122	observations	1111:1122	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	8	40	theme	ASKG	1220:1223	arg1	addition					1225:1232	0.3% ASKG addition	1215:1232	0.3% ASKG addition	1215:1232	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	4	41	from	level	550:554	arg1	trend					534:538	a decreased trend	522:538	a decreased trend at higher level of gum addition (0.8%)	522:577	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	4	42	theme	addition	563:570	arg1	level					550:554	higher level	543:554	higher level of gum addition (0.8%)	543:577	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	5	43	theme	relative	674:681	arg1	crystallinity					683:695	relative crystallinity	674:695	relative crystallinity measured by X-ray diffractometer for dough samples	674:746	This trend was in agreement with the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples.					
32057874	2	44	theme	sphaerocephala	202:215	arg1	effect					182:187	The effect	178:187	The effect of Artemisia sphaerocephala	178:215	The effect of Artemisia sphaerocephala Krasch.					
32057874	8	45	theme	0.3	1215:1217	arg1	%					1218:1218	%	1218:1218	%	1218:1218	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	0	46	theme	structural	31:40	arg1	characteristics					42:56	structural characteristics	31:56	structural characteristics	31:56	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	3	47	dep	performance	258:268	arg1	the					248:250	the	248:250	the	248:250	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	2	48	theme	Artemisia	192:200	arg1	sphaerocephala					202:215	Artemisia sphaerocephala	192:215	Artemisia sphaerocephala	192:215	The effect of Artemisia sphaerocephala Krasch.					
32057874	0	49	theme	chickpea-wheat	61:74	arg1	noodles					98:104	chickpea-wheat composite flour-based noodles	61:104	chickpea-wheat composite flour-based noodles	61:104	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	3	50	from	addition	236:243	arg1	performance					258:268	dough performance	252:268	dough performance	252:268	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	3	50	from	addition	236:243	arg1	characteristics					285:299	structural characteristics	274:299	structural characteristics	274:299	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	3	51	theme	product	345:351	arg1	performance					258:268	dough performance	252:268	dough performance	252:268	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	3	51	theme	product	345:351	arg1	characteristics					285:299	structural characteristics	274:299	structural characteristics	274:299	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	5	52	theme	dough	734:738	arg1	samples					740:746	dough samples	734:746	dough samples	734:746	This trend was in agreement with the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples.					
32057874	9	53	from	%	1307:1307	arg1	levels					1265:1270	the higher levels	1254:1270	the higher levels of gum substitution at 0.5% and 0.8%	1254:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	9	53	from	%	1307:1307	arg1	substitution					1279:1290	gum substitution	1275:1290	gum substitution at 0.5% and 0.8%	1275:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	0	54	with	rheology	18:25	arg1	levels					121:126	different levels	111:126	different levels of Artemisia sphaerocephala Krasch	111:161	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	9	55	theme	inefficient	1402:1412	arg1	entrapment					1414:1423	the inefficient entrapment	1398:1423	the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network	1398:1500	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	5	56	theme	free	652:655	arg1	content					661:667	free -SH content	652:667	free -SH content	652:667	This trend was in agreement with the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples.					
32057874	6	57	theme	above	818:822	arg1	trend					824:828	the above trend	814:828	the above trend	814:828	The confocal laser scanning micrographs (CLSM) further confirmed the above trend.					
32057874	7	58	theme	addition	1058:1065	arg1	%					1049:1049	the 0.3%	1042:1049	the 0.3% of gum addition	1042:1065	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	7	58	theme	addition	1058:1065	arg1	addition					1058:1065	gum addition	1054:1065	gum addition	1054:1065	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	4	59	theme	addition	389:396	arg1	levels					374:379	The low levels	366:379	The low levels of ASKG addition (0.03-0.5%)	366:408	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	8	60	theme	network	1181:1187	arg1	structure					1189:1197	a relatively denser and more coherent network structure	1143:1197	a relatively denser and more coherent network structure	1143:1197	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	7	61	theme	pasting	876:882	arg1	properties					884:893	pasting properties	876:893	pasting properties	876:893	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	4	62	theme	higher	543:548	arg1	level					550:554	higher level	543:554	higher level of gum addition (0.8%)	543:577	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	3	63	theme	composite	323:331	arg1	product					345:351	the wheat-chickpea composite flour-based product	304:351	the wheat-chickpea composite flour-based product	304:351	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	4	64	theme	remarkable	428:437	arg1	increase					439:446	a remarkable increase	426:446	a remarkable increase	426:446	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	4	65	theme	ASKG	384:387	arg1	%					407:407	0.03-0.5%	399:407	0.03-0.5%	399:407	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	4	65	theme	ASKG	384:387	arg1	addition					389:396	ASKG addition	384:396	ASKG addition (0.03-0.5%)	384:408	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	5	66	with	agreement	598:606	arg1	variations					617:626	the variations	613:626	the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples	613:746	This trend was in agreement with the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples.					
32057874	4	67	theme	low	370:372	arg1	levels					374:379	The low levels	366:379	The low levels of ASKG addition (0.03-0.5%)	366:408	The low levels of ASKG addition (0.03-0.5%) tended to cause a remarkable increase in the viscoelastic properties of the composite dough system, followed by a decreased trend at higher level of gum addition (0.8%).					
32057874	3	68	theme	flour-based	333:343	arg1	product					345:351	the wheat-chickpea composite flour-based product	304:351	the wheat-chickpea composite flour-based product	304:351	gum (ASKG) addition on the dough performance and structural characteristics of the wheat-chickpea composite flour-based product was studied.					
32057874	7	69	theme	sulfhydryl	961:970	arg1	content					972:978	free sulfhydryl content	956:978	free sulfhydryl content	956:978	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	9	70	theme	starch	1371:1376	arg1	deformation					1378:1388	starch deformation	1371:1388	starch deformation	1371:1388	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	8	71	theme	coherent	1172:1179	arg1	structure					1189:1197	a relatively denser and more coherent network structure	1143:1197	a relatively denser and more coherent network structure	1143:1197	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	0	72	theme	flour-based	86:96	arg1	noodles					98:104	chickpea-wheat composite flour-based noodles	61:104	chickpea-wheat composite flour-based noodles	61:104	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	7	73	dep	%	1049:1049	arg1	formed					1032:1037	formed	1032:1037	was formed at the 0.3% of gum addition	1028:1065	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	9	74	theme	molecules	1435:1443	arg1	entrapment					1414:1423	the inefficient entrapment	1398:1423	the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network	1398:1500	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	7	75	from	changes	916:922	arg1	content					972:978	free sulfhydryl content	956:978	free sulfhydryl content	956:978	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	7	75	from	changes	916:922	arg1	crystallinity					938:950	crystallinity	938:950	crystallinity	938:950	For cooked noodle samples, the variations in pasting properties was supported by the changes in relatively crystallinity and free sulfhydryl content, suggesting a relatively more compact structure was formed at the 0.3% of gum addition.					
32057874	5	76	theme	-SH	657:659	arg1	content					661:667	free -SH content	652:667	free -SH content	652:667	This trend was in agreement with the variations found for cooking loss, free -SH content, and relative crystallinity measured by X-ray diffractometer for dough samples.					
32057874	0	77	theme	composite	76:84	arg1	noodles					98:104	chickpea-wheat composite flour-based noodles	61:104	chickpea-wheat composite flour-based noodles	61:104	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	9	78	theme	gluten	1487:1492	arg1	network					1494:1500	gluten network	1487:1500	gluten network	1487:1500	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	0	79	with	characteristics	42:56	arg1	levels					121:126	different levels	111:126	different levels of Artemisia sphaerocephala Krasch	111:161	Controlling dough rheology and structural characteristics of chickpea-wheat composite flour-based noodles with different levels of Artemisia sphaerocephala Krasch.					
32057874	8	80	theme	denser	1156:1161	arg1	structure					1189:1197	a relatively denser and more coherent network structure	1143:1197	a relatively denser and more coherent network structure	1143:1197	The scanning electron microscopic and CLSM observations both revealed that a relatively denser and more coherent network structure was achieved at 0.3% ASKG addition.					
32057874	9	81	from	levels	1265:1270	arg1	%					1298:1298	0.5%	1295:1298	0.5%	1295:1298	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
32057874	9	81	from	levels	1265:1270	arg1	%					1307:1307	0.8%	1304:1307	0.8%	1304:1307	On the other hand, the higher levels of gum substitution at 0.5% and 0.8% tended to disrupt this stronger network with visible signs of starch deformation, due to the inefficient entrapment of starch molecules during cooking as a result of the lack of gluten network.					
34523263	0	0	theme	In	95:96	arg1	Regeneration					119:130	In Situ Arterial Tissue Regeneration	95:130	In Situ Arterial Tissue Regeneration	95:130	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	2	1	theme	grafts	534:539	arg1	performance					475:485	the in vivo performance	463:485	the in vivo performance of electrospun resorbable elastomeric vascular grafts	463:539	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	2	2	theme	supramolecular	650:663	arg1	approach					665:672	a supramolecular approach	648:672	a supramolecular approach	648:672	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	3	3	from	graft	773:777	arg1	aorta					800:804	the rat abdominal aorta	782:804	the rat abdominal aorta	782:804	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	9	4	theme	graft	1542:1546	arg1	outcomes					1548:1555	graft outcomes	1542:1555	graft outcomes	1542:1555	Nevertheless, variability between graft outcomes is observed within each group, warranting further evaluation in light of clinical translation.					
34523263	4	5	theme	tissue	1048:1053	arg1	fingerprints					994:1005	the local molecular fingerprints	974:1005	the local molecular fingerprints of the resorbing scaffold and developing tissue	974:1053	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	1	6	theme	tissue-engineered	273:289	arg1	grafts					300:305	small-diameter in situ tissue-engineered vascular grafts	250:305	small-diameter in situ tissue-engineered vascular grafts	250:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	2	7	theme	vascular	525:532	arg1	grafts					534:539	electrospun resorbable elastomeric vascular grafts	490:539	electrospun resorbable elastomeric vascular grafts	490:539	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	0	8	from	Effects	9:15	arg1	Polarization					78:89	Macrophage Polarization	67:89	Macrophage Polarization	67:89	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	0	8	from	Effects	9:15	arg1	Regeneration					119:130	In Situ Arterial Tissue Regeneration	95:130	In Situ Arterial Tissue Regeneration	95:130	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	1	9	theme	vascular	291:298	arg1	grafts					300:305	small-diameter in situ tissue-engineered vascular grafts	250:305	small-diameter in situ tissue-engineered vascular grafts	250:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	0	10	theme	Arterial	103:110	arg1	Regeneration					119:130	In Situ Arterial Tissue Regeneration	95:130	In Situ Arterial Tissue Regeneration	95:130	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	9	11	theme	translation	1639:1649	arg1	light					1621:1625	light	1621:1625	light of clinical translation	1621:1649	Nevertheless, variability between graft outcomes is observed within each group, warranting further evaluation in light of clinical translation.					
34523263	3	12	theme	IL-4/hep	704:711	arg1	grafts					733:738	IL-4/hep, hep-only, and bare grafts	704:738	IL-4/hep, hep-only, and bare grafts	704:738	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	8	13	theme	early	1422:1426	arg1	response					1441:1448	the early inflammatory response	1418:1448	the early inflammatory response	1418:1448	This study shows that with bioactive functionalization, the early inflammatory response can be modulated and affect the composition of neotissue.					
34523263	1	14	theme	grafts	300:305	arg1	application					235:245	successful application	224:245	successful application of small-diameter in situ tissue-engineered vascular grafts	224:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	3	15	theme	grafts	733:738	arg1	graft					773:777	interposition graft	759:777	interposition graft in the rat abdominal aorta	759:804	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	15	theme	grafts	733:738	arg1	capacity					692:699	The regenerative capacity	675:699	The regenerative capacity of IL-4/hep, hep-only, and bare grafts	675:738	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	6	16	theme	tissue	1180:1185	arg1	remodeling					1187:1196	adverse tissue remodeling	1172:1196	adverse tissue remodeling	1172:1196	Hep-only-functionalized grafts resulted in adverse tissue remodeling, with cases of local intimal hyperplasia.					
34523263	4	17	theme	molecular	984:992	arg1	fingerprints					994:1005	the local molecular fingerprints	974:1005	the local molecular fingerprints of the resorbing scaffold and developing tissue	974:1053	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	0	18	from	Grafts	173:178	arg1	Rats					183:186	Rats	183:186	Rats	183:186	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	2	19	theme	anti-thrombogenic	567:583	arg1	hep					594:596	hep	594:596	hep	594:596	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	2	19	theme	anti-thrombogenic	567:583	arg1	heparin					585:591	anti-thrombogenic heparin	567:591	anti-thrombogenic heparin (hep)	567:597	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	5	20	theme	confounding	1088:1098	arg1	factor					1100:1105	a confounding factor	1086:1105	a confounding factor	1086:1105	Thrombosis is found not to be a confounding factor in any of the groups.					
34523263	2	21	dep	in	467:468	arg1	vivo					470:473	vivo	470:473	vivo	470:473	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	3	22	theme	healing	847:853	arg1	cascade					855:861	the healing cascade	843:861	the healing cascade	843:861	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	6	23	theme	adverse	1172:1178	arg1	remodeling					1187:1196	adverse tissue remodeling	1172:1196	adverse tissue remodeling	1172:1196	Hep-only-functionalized grafts resulted in adverse tissue remodeling, with cases of local intimal hyperplasia.					
34523263	0	24	theme	Tissue	112:117	arg1	Regeneration					119:130	In Situ Arterial Tissue Regeneration	95:130	In Situ Arterial Tissue Regeneration	95:130	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	3	25	theme	interposition	759:771	arg1	graft					773:777	interposition graft	759:777	interposition graft in the rat abdominal aorta	759:804	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	25	theme	interposition	759:771	arg1	capacity					692:699	The regenerative capacity	675:699	The regenerative capacity of IL-4/hep, hep-only, and bare grafts	675:738	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	2	26	theme	elastomeric	513:523	arg1	grafts					534:539	electrospun resorbable elastomeric vascular grafts	490:539	electrospun resorbable elastomeric vascular grafts	490:539	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	0	27	theme	Distinct	0:7	arg1	Effects					9:15	Distinct Effects	0:15	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.	0:187	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	4	28	theme	Raman	929:933	arg1	microspectroscopy					935:951	Raman microspectroscopy	929:951	Raman microspectroscopy	929:951	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	4	29	theme	scaffold	1024:1031	arg1	fingerprints					994:1005	the local molecular fingerprints	974:1005	the local molecular fingerprints of the resorbing scaffold and developing tissue	974:1053	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	3	30	theme	abdominal	790:798	arg1	aorta					800:804	the rat abdominal aorta	782:804	the rat abdominal aorta	782:804	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	0	31	dep	In	95:96	arg1	Situ					98:101	Situ	98:101	Situ	98:101	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	7	32	theme	macrophage	1301:1310	arg1	polarization					1312:1323	M2 macrophage polarization	1298:1323	M2 macrophage polarization	1298:1323	This is negated with the addition of IL-4, which promoted M2 macrophage polarization and more mature neotissue formation.					
34523263	1	33	theme	inflammatory	366:377	arg1	response					379:386	the inflammatory response	362:386	the inflammatory response to the graft to guide functional tissue regeneration	362:439	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	4	34	theme	resorbing	1014:1022	arg1	scaffold					1024:1031	the resorbing scaffold and developing tissue	1010:1053	scaffold	1024:1031	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	1	35	theme	greatest	200:207	arg1	challenges					209:218	the greatest challenges	196:218	the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts	196:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	0	36	theme	Heparin	20:26	arg1	Effects					9:15	Distinct Effects	0:15	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.	0:187	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	7	37	theme	mature	1334:1339	arg1	formation					1351:1359	more mature neotissue formation	1329:1359	more mature neotissue formation	1329:1359	This is negated with the addition of IL-4, which promoted M2 macrophage polarization and more mature neotissue formation.					
34523263	0	38	theme	Resorbable	138:147	arg1	Grafts					173:178	Resorbable Supramolecular Vascular Grafts	138:178	Resorbable Supramolecular Vascular Grafts in Rats	138:186	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	6	39	theme	Hep-only-functionalized	1129:1151	arg1	grafts					1153:1158	Hep-only-functionalized grafts	1129:1158	Hep-only-functionalized grafts	1129:1158	Hep-only-functionalized grafts resulted in adverse tissue remodeling, with cases of local intimal hyperplasia.					
34523263	4	40	theme	local	978:982	arg1	fingerprints					994:1005	the local molecular fingerprints	974:1005	the local molecular fingerprints of the resorbing scaffold and developing tissue	974:1053	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	1	41	theme	challenges	209:218	arg1	Two					189:191	Two	189:191	Two	189:191	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	1	41	theme	challenges	209:218	arg1	challenges					209:218	the greatest challenges	196:218	the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts	196:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	1	42	dep	harnessing	351:360	arg1	2					348:348	2	348:348	2	348:348	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	1	43	dep	in	265:266	arg1	situ					268:271	situ	268:271	situ	268:271	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	9	44	theme	further	1599:1605	arg1	evaluation					1607:1616	further evaluation	1599:1616	further evaluation	1599:1616	Nevertheless, variability between graft outcomes is observed within each group, warranting further evaluation in light of clinical translation.					
34523263	7	45	theme	M2	1298:1299	arg1	polarization					1312:1323	M2 macrophage polarization	1298:1323	M2 macrophage polarization	1298:1323	This is negated with the addition of IL-4, which promoted M2 macrophage polarization and more mature neotissue formation.					
34523263	0	46	theme	Interleukin-4	32:44	arg1	Effects					9:15	Distinct Effects	0:15	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.	0:187	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	1	47	theme	thrombus	325:332	arg1	formation					334:342	thrombus formation	325:342	thrombus formation	325:342	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	0	48	theme	Vascular	164:171	arg1	Grafts					173:178	Resorbable Supramolecular Vascular Grafts	138:178	Resorbable Supramolecular Vascular Grafts in Rats	138:186	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	1	49	theme	functional	410:419	arg1	regeneration					428:439	functional tissue regeneration	410:439	functional tissue regeneration	410:439	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	8	50	theme	bioactive	1389:1397	arg1	functionalization					1399:1415	bioactive functionalization	1389:1415	bioactive functionalization	1389:1415	This study shows that with bioactive functionalization, the early inflammatory response can be modulated and affect the composition of neotissue.					
34523263	4	51	theme	developing	1037:1046	arg1	tissue					1048:1053	the resorbing scaffold and developing tissue	1010:1053	tissue	1048:1053	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	1	52	theme	successful	224:233	arg1	application					235:245	successful application	224:245	successful application of small-diameter in situ tissue-engineered vascular grafts	224:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	2	53	theme	resorbable	502:511	arg1	grafts					534:539	electrospun resorbable elastomeric vascular grafts	490:539	electrospun resorbable elastomeric vascular grafts	490:539	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	0	54	theme	Supramolecular	149:162	arg1	Grafts					173:178	Resorbable Supramolecular Vascular Grafts	138:178	Resorbable Supramolecular Vascular Grafts in Rats	138:186	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	1	55	theme	tissue	421:426	arg1	regeneration					428:439	functional tissue regeneration	410:439	functional tissue regeneration	410:439	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	2	56	theme	in	467:468	arg1	performance					475:485	the in vivo performance	463:485	the in vivo performance of electrospun resorbable elastomeric vascular grafts	463:539	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	2	57	theme	electrospun	490:500	arg1	grafts					534:539	electrospun resorbable elastomeric vascular grafts	490:539	electrospun resorbable elastomeric vascular grafts	490:539	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	3	58	theme	key	825:827	arg1	timepoints					829:838	key timepoints	825:838	key timepoints in the healing cascade (1, 3, 7 days, and 3 months)	825:890	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	59	from	timepoints	829:838	arg1	follow-up					812:820	follow-up	812:820	follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months)	812:890	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	59	from	timepoints	829:838	arg1	cascade					855:861	the healing cascade	843:861	the healing cascade	843:861	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	6	60	theme	intimal	1219:1225	arg1	hyperplasia					1227:1237	local intimal hyperplasia	1213:1237	local intimal hyperplasia	1213:1237	Hep-only-functionalized grafts resulted in adverse tissue remodeling, with cases of local intimal hyperplasia.					
34523263	2	61	theme	anti-inflammatory	603:619	arg1	interleukin					621:631	anti-inflammatory interleukin 4	603:633	anti-inflammatory interleukin 4 (IL-4)	603:640	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	2	61	theme	anti-inflammatory	603:619	arg1	IL-4					636:639	IL-4	636:639	IL-4	636:639	This study evaluates the in vivo performance of electrospun resorbable elastomeric vascular grafts, dual-functionalized with anti-thrombogenic heparin (hep) and anti-inflammatory interleukin 4 (IL-4) using a supramolecular approach.					
34523263	6	62	theme	local	1213:1217	arg1	hyperplasia					1227:1237	local intimal hyperplasia	1213:1237	local intimal hyperplasia	1213:1237	Hep-only-functionalized grafts resulted in adverse tissue remodeling, with cases of local intimal hyperplasia.					
34523263	7	63	theme	IL-4	1277:1280	arg1	addition					1265:1272	the addition	1261:1272	the addition	1261:1272	This is negated with the addition of IL-4, which promoted M2 macrophage polarization and more mature neotissue formation.					
34523263	8	64	theme	inflammatory	1428:1439	arg1	response					1441:1448	the early inflammatory response	1418:1448	the early inflammatory response	1418:1448	This study shows that with bioactive functionalization, the early inflammatory response can be modulated and affect the composition of neotissue.					
34523263	3	65	theme	bare	728:731	arg1	grafts					733:738	IL-4/hep, hep-only, and bare grafts	704:738	IL-4/hep, hep-only, and bare grafts	704:738	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	0	66	dep	Heparin	20:26	arg1	Functionalization					46:62	Functionalization	46:62	Functionalization	46:62	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	6	67	theme	hyperplasia	1227:1237	arg1	cases					1204:1208	cases	1204:1208	cases of local intimal hyperplasia	1204:1237	Hep-only-functionalized grafts resulted in adverse tissue remodeling, with cases of local intimal hyperplasia.					
34523263	3	68	theme	rat	786:788	arg1	aorta					800:804	the rat abdominal aorta	782:804	the rat abdominal aorta	782:804	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	4	69	theme	Routine	893:899	arg1	analyses					901:908	Routine analyses	893:908	Routine analyses	893:908	Routine analyses are augmented with Raman microspectroscopy, in order to acquire the local molecular fingerprints of the resorbing scaffold and developing tissue.					
34523263	3	70	theme	hep-only	714:721	arg1	grafts					733:738	IL-4/hep, hep-only, and bare grafts	704:738	IL-4/hep, hep-only, and bare grafts	704:738	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	71	theme	regenerative	679:690	arg1	graft					773:777	interposition graft	759:777	interposition graft in the rat abdominal aorta	759:804	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	71	theme	regenerative	679:690	arg1	capacity					692:699	The regenerative capacity	675:699	The regenerative capacity of IL-4/hep, hep-only, and bare grafts	675:738	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	8	72	theme	neotissue	1497:1505	arg1	composition					1482:1492	the composition	1478:1492	the composition of neotissue	1478:1505	This study shows that with bioactive functionalization, the early inflammatory response can be modulated and affect the composition of neotissue.					
34523263	1	73	theme	small-diameter	250:263	arg1	grafts					300:305	small-diameter in situ tissue-engineered vascular grafts	250:305	small-diameter in situ tissue-engineered vascular grafts	250:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	9	74	located	observed	1560:1567	arg2	variability					1522:1532	variability	1522:1532	variability between graft outcomes	1522:1555	Nevertheless, variability between graft outcomes is observed within each group, warranting further evaluation in light of clinical translation.					
34523263	9	74	located	observed	1560:1567	arg1	group					1581:1585	each group	1576:1585	each group	1576:1585	Nevertheless, variability between graft outcomes is observed within each group, warranting further evaluation in light of clinical translation.					
34523263	3	75	dep	timepoints	829:838	arg1	3					867:867	3	867:867	3	867:867	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	75	dep	timepoints	829:838	arg1	days					872:875	7 days	870:875	7 days	870:875	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	75	dep	timepoints	829:838	arg1	1					864:864	1	864:864	1	864:864	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	3	75	dep	timepoints	829:838	arg1	months					884:889	3 months	882:889	3 months	882:889	The regenerative capacity of IL-4/hep, hep-only, and bare grafts is investigated as interposition graft in the rat abdominal aorta, with follow-up at key timepoints in the healing cascade (1, 3, 7 days, and 3 months).					
34523263	9	76	theme	clinical	1630:1637	arg1	translation					1639:1649	clinical translation	1630:1649	clinical translation	1630:1649	Nevertheless, variability between graft outcomes is observed within each group, warranting further evaluation in light of clinical translation.					
34523263	1	77	theme	in	265:266	arg1	grafts					300:305	small-diameter in situ tissue-engineered vascular grafts	250:305	small-diameter in situ tissue-engineered vascular grafts	250:305	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	0	78	theme	Macrophage	67:76	arg1	Polarization					78:89	Macrophage Polarization	67:89	Macrophage Polarization	67:89	Distinct Effects of Heparin and Interleukin-4 Functionalization on Macrophage Polarization and In Situ Arterial Tissue Regeneration Using Resorbable Supramolecular Vascular Grafts in Rats.					
34523263	1	79	dep	preventing	314:323	arg1	1					311:311	1	311:311	1	311:311	Two of the greatest challenges for successful application of small-diameter in situ tissue-engineered vascular grafts are 1) preventing thrombus formation and 2) harnessing the inflammatory response to the graft to guide functional tissue regeneration.					
34523263	7	80	theme	neotissue	1341:1349	arg1	formation					1351:1359	more mature neotissue formation	1329:1359	more mature neotissue formation	1329:1359	This is negated with the addition of IL-4, which promoted M2 macrophage polarization and more mature neotissue formation.					
34523263	5	81	from	factor	1100:1105	arg1	any					1110:1112	any	1110:1112	any	1110:1112	Thrombosis is found not to be a confounding factor in any of the groups.					
33892030	6	0	theme	hydrogels	561:569	arg1	modulus					517:523	tensile modulus	509:523	tensile modulus	509:523	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	6	0	theme	hydrogels	561:569	arg1	elongation					529:538	elongation	529:538	elongation	529:538	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	5	1	theme	SA	429:430	arg1	addition					417:424	The addition	413:424	The addition of SA	413:430	The addition of SA significantly improves the mechanical properties of composite hydrogels.					
33892030	3	2	theme	natural	330:336	arg1	materials					346:354	natural polymer materials	330:354	natural polymer materials	330:354	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	2	theme	natural	330:336	arg1	gelatin					289:295	gelatin	289:295	gelatin (G)	289:299	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	2	theme	natural	330:336	arg1	alginate					312:319	sodium alginate	305:319	sodium alginate (SA)	305:324	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	6	3	theme	2.0	576:578	arg1	%					579:579	%	579:579	%	579:579	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	11	4	theme	EDTA-induced	1150:1161	arg1	performances					1174:1185	EDTA-induced detachment performances	1150:1185	EDTA-induced detachment performances	1150:1185	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	7	5	from	addition	649:656	arg1	beneficial					740:749	beneficial	740:749	beneficial	740:749	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	5	from	addition	649:656	arg1	network					685:691	the interconnected porous network	659:691	the interconnected porous network	659:691	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	6	from	beneficial	740:749	arg1	addition					649:656	addition	649:656	addition	649:656	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	12	7	theme	G/SA	1346:1349	arg1	hydrogels					1351:1359	carried G/SA hydrogels	1338:1359	carried G/SA hydrogels	1338:1359	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	12	8	theme	wound	1384:1388	arg1	materials					1399:1407	wound dressing materials	1384:1407	wound dressing materials	1384:1407	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	7	9	theme	wound	837:841	arg1	healing					826:832	the healing	822:832	the healing of wound	822:841	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	10	theme	high	697:700	arg1	ratio					711:715	high swelling ratio	697:715	high swelling ratio (over 9.99 ± 0.33)	697:734	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	10	theme	high	697:700	arg1	±					728:728	over 9.99 ± 0.33	718:733	over 9.99 ± 0.33	718:733	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	11	11	theme	performances	1174:1185	arg1	results					1139:1145	results	1139:1145	results of EDTA-induced detachment performances	1139:1185	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	6	12	from	break	543:547	arg1	modulus					517:523	tensile modulus	509:523	tensile modulus	509:523	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	6	12	from	break	543:547	arg1	elongation					529:538	elongation	529:538	elongation	529:538	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	7	13	theme	swelling	702:709	arg1	ratio					711:715	high swelling ratio	697:715	high swelling ratio (over 9.99 ± 0.33)	697:734	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	13	theme	swelling	702:709	arg1	±					728:728	over 9.99 ± 0.33	718:733	over 9.99 ± 0.33	718:733	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	10	14	theme	cells	1056:1060	arg1	results					1040:1046	The results	1036:1046	The results of L929 cells cultured with the hydrogel extracts	1036:1096	The results of L929 cells cultured with the hydrogel extracts indicate good biocompatibility.					
33892030	6	15	theme	±	623:623	arg1	%					629:629	99.23 ± 2.18 kPa and 85.47 ± 5.01%	596:629	%	629:629	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	6	16	theme	%	579:579	arg1	SA					581:582	2.0% SA	576:582	2.0% SA	576:582	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	10	17	theme	L929	1051:1054	arg1	cells					1056:1060	L929 cells	1051:1060	L929 cells cultured with the hydrogel extracts	1051:1096	The results of L929 cells cultured with the hydrogel extracts indicate good biocompatibility.					
33892030	9	18	theme	sustained	938:946	arg1	release					953:959	sustained drug release	938:959	sustained drug release for 168 h	938:969	The BBR/G/SA hydrogels show sustained drug release for 168 h and exhibit anti-bacterial effect against Staphylococcus aureus.					
33892030	11	19	theme	detachment	1163:1172	arg1	performances					1174:1185	EDTA-induced detachment performances	1150:1185	EDTA-induced detachment performances	1150:1185	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	12	20	theme	dressing	1390:1397	arg1	materials					1399:1407	wound dressing materials	1384:1407	wound dressing materials	1384:1407	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	6	21	theme	G/SA	556:559	arg1	hydrogels					561:569	the G/SA hydrogels	552:569	the G/SA hydrogels with 2.0% SA	552:582	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	0	22	theme	detachment	88:97	arg1	performances					99:110	antibacterial and EDTA-induced detachment performances	57:110	antibacterial and EDTA-induced detachment performances	57:110	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	1	23	theme	Most	113:116	arg1	dressings					142:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings lack gentle detachment property.					
33892030	11	24	attach	removed	1227:1233	arg1	wound					1244:1248	the wound	1240:1248	the wound	1240:1248	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	11	24	attach	removed	1227:1233	arg2	structure					1266:1274	the internal structure	1253:1274	the internal structure destroyed	1253:1284	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	11	24	attach	removed	1227:1233	arg2	hydrogels					1208:1216	the hydrogels	1204:1216	the hydrogels	1204:1216	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	9	25	theme	BBR/G/SA	914:921	arg1	hydrogels					923:931	The BBR/G/SA hydrogels	910:931	The BBR/G/SA hydrogels	910:931	The BBR/G/SA hydrogels show sustained drug release for 168 h and exhibit anti-bacterial effect against Staphylococcus aureus.					
33892030	4	26	theme	dual	393:396	arg1	cross-linking					398:410	dual cross-linking	393:410	dual cross-linking	393:410	The G/SA hydrogels were prepared by dual cross-linking.					
33892030	1	27	theme	existing	118:125	arg1	dressings					142:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings lack gentle detachment property.					
33892030	5	28	theme	composite	484:492	arg1	hydrogels					494:502	composite hydrogels	484:502	composite hydrogels	484:502	The addition of SA significantly improves the mechanical properties of composite hydrogels.					
33892030	11	29	theme	internal	1257:1264	arg1	hydrogels					1208:1216	the hydrogels	1204:1216	the hydrogels	1204:1216	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	11	29	theme	internal	1257:1264	arg1	structure					1266:1274	the internal structure	1253:1274	the internal structure destroyed	1253:1284	Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed.					
33892030	0	30	theme	alginate	33:40	arg1	hydrogels					42:50	gelatin/sodium alginate hydrogels	18:50	gelatin/sodium alginate hydrogels	18:50	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	7	31	theme	oxygen	774:779	arg1	transmission					758:769	the transmission	754:769	the transmission of oxygen and absorption of exudates to accelerate the healing of wound	754:841	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	0	32	contain	carried	10:16	arg1	Berberine					0:8	Berberine	0:8	Berberine	0:8	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	0	32	contain	carried	10:16	arg2	hydrogels					42:50	gelatin/sodium alginate hydrogels	18:50	gelatin/sodium alginate hydrogels	18:50	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	1	33	theme	hydrogel	127:134	arg1	dressings					142:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings lack gentle detachment property.					
33892030	5	34	theme	hydrogels	494:502	arg1	properties					470:479	the mechanical properties	455:479	the mechanical properties of composite hydrogels	455:502	The addition of SA significantly improves the mechanical properties of composite hydrogels.					
33892030	0	35	theme	gelatin/sodium	18:31	arg1	hydrogels					42:50	gelatin/sodium alginate hydrogels	18:50	gelatin/sodium alginate hydrogels	18:50	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	3	36	theme	sodium	305:310	arg1	gelatin					289:295	gelatin	289:295	gelatin (G)	289:299	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	36	theme	sodium	305:310	arg1	materials					346:354	natural polymer materials	330:354	natural polymer materials	330:354	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	36	theme	sodium	305:310	arg1	SA					322:323	SA	322:323	SA	322:323	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	36	theme	sodium	305:310	arg1	alginate					312:319	sodium alginate	305:319	sodium alginate (SA)	305:324	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	1	37	theme	wound	136:140	arg1	dressings					142:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings	113:150	Most existing hydrogel wound dressings lack gentle detachment property.					
33892030	6	38	dep	modulus	517:523	arg1	The					505:507	The	505:507	The	505:507	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	2	39	theme	novel	199:203	arg1	hydrogels					205:213	novel hydrogels	199:213	novel hydrogels with anti-bacterial and induced detachment properties	199:267	In this work, novel hydrogels with anti-bacterial and induced detachment properties were prepared.					
33892030	7	40	theme	absorption	785:794	arg1	transmission					758:769	the transmission	754:769	the transmission of oxygen and absorption of exudates to accelerate the healing of wound	754:841	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	12	41	dep	BBR	1334:1336	arg1	have					1361:1364	have	1361:1364	have potential used as wound dressing materials in future	1361:1417	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	9	42	theme	drug	948:951	arg1	release					953:959	sustained drug release	938:959	sustained drug release for 168 h	938:969	The BBR/G/SA hydrogels show sustained drug release for 168 h and exhibit anti-bacterial effect against Staphylococcus aureus.					
33892030	6	43	theme	tensile	509:515	arg1	modulus					517:523	tensile modulus	509:523	tensile modulus	509:523	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	7	44	theme	interconnected	663:676	arg1	beneficial					740:749	beneficial	740:749	beneficial	740:749	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	44	theme	interconnected	663:676	arg1	network					685:691	the interconnected porous network	659:691	the interconnected porous network	659:691	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	6	45	theme	±	602:602	arg1	kPa					609:611	99.23 ± 2.18 kPa and 85.47 ± 5.01%	596:629	kPa	609:611	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	6	46	with	hydrogels	561:569	arg1	SA					581:582	2.0% SA	576:582	2.0% SA	576:582	The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively.					
33892030	7	47	theme	exudates	799:806	arg1	absorption					785:794	absorption	785:794	absorption	785:794	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	47	theme	exudates	799:806	arg1	oxygen					774:779	oxygen	774:779	oxygen	774:779	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	48	theme	porous	678:683	arg1	beneficial					740:749	beneficial	740:749	beneficial	740:749	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	7	48	theme	porous	678:683	arg1	network					685:691	the interconnected porous network	659:691	the interconnected porous network	659:691	In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound.					
33892030	12	49	contain	have	1361:1364	arg1	hydrogels					1351:1359	carried G/SA hydrogels	1338:1359	carried G/SA hydrogels	1338:1359	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	12	49	contain	have	1361:1364	arg2	potential					1366:1374	potential	1366:1374	potential used as wound dressing materials	1366:1407	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	0	50	theme	antibacterial	57:69	arg1	performances					99:110	antibacterial and EDTA-induced detachment performances	57:110	antibacterial and EDTA-induced detachment performances	57:110	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	10	51	theme	good	1107:1110	arg1	biocompatibility					1112:1127	good biocompatibility	1107:1127	good biocompatibility	1107:1127	The results of L929 cells cultured with the hydrogel extracts indicate good biocompatibility.					
33892030	2	52	theme	induced	239:245	arg1	properties					258:267	anti-bacterial and induced detachment properties	220:267	anti-bacterial and induced detachment properties	220:267	In this work, novel hydrogels with anti-bacterial and induced detachment properties were prepared.					
33892030	2	53	theme	detachment	247:256	arg1	properties					258:267	anti-bacterial and induced detachment properties	220:267	anti-bacterial and induced detachment properties	220:267	In this work, novel hydrogels with anti-bacterial and induced detachment properties were prepared.					
33892030	4	54	theme	G/SA	361:364	arg1	hydrogels					366:374	The G/SA hydrogels	357:374	The G/SA hydrogels	357:374	The G/SA hydrogels were prepared by dual cross-linking.					
33892030	1	55	theme	gentle	157:162	arg1	property					175:182	gentle detachment property	157:182	gentle detachment property	157:182	Most existing hydrogel wound dressings lack gentle detachment property.					
33892030	5	56	theme	mechanical	459:468	arg1	properties					470:479	the mechanical properties	455:479	the mechanical properties of composite hydrogels	455:502	The addition of SA significantly improves the mechanical properties of composite hydrogels.					
33892030	2	57	theme	anti-bacterial	220:233	arg1	properties					258:267	anti-bacterial and induced detachment properties	220:267	anti-bacterial and induced detachment properties	220:267	In this work, novel hydrogels with anti-bacterial and induced detachment properties were prepared.					
33892030	12	58	theme	illustrated	1291:1301	arg1	results					1303:1309	All illustrated results	1287:1309	All illustrated results	1287:1309	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	8	59	theme	G/SA	894:897	arg1	hydrogels					899:907	the G/SA hydrogels	890:907	the G/SA hydrogels	890:907	Subsequently, berberine (BBR) was loaded into the G/SA hydrogels.					
33892030	1	60	theme	detachment	164:173	arg1	property					175:182	gentle detachment property	157:182	gentle detachment property	157:182	Most existing hydrogel wound dressings lack gentle detachment property.					
33892030	2	61	with	hydrogels	205:213	arg1	properties					258:267	anti-bacterial and induced detachment properties	220:267	anti-bacterial and induced detachment properties	220:267	In this work, novel hydrogels with anti-bacterial and induced detachment properties were prepared.					
33892030	3	62	theme	polymer	338:344	arg1	materials					346:354	natural polymer materials	330:354	natural polymer materials	330:354	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	62	theme	polymer	338:344	arg1	gelatin					289:295	gelatin	289:295	gelatin (G)	289:299	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	3	62	theme	polymer	338:344	arg1	alginate					312:319	sodium alginate	305:319	sodium alginate (SA)	305:324	Both gelatin (G) and sodium alginate (SA) are natural polymer materials.					
33892030	12	63	theme	carried	1338:1344	arg1	hydrogels					1351:1359	carried G/SA hydrogels	1338:1359	carried G/SA hydrogels	1338:1359	All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.					
33892030	10	64	theme	hydrogel	1080:1087	arg1	extracts					1089:1096	the hydrogel extracts	1076:1096	the hydrogel extracts	1076:1096	The results of L929 cells cultured with the hydrogel extracts indicate good biocompatibility.					
33892030	0	65	theme	EDTA-induced	75:86	arg1	performances					99:110	antibacterial and EDTA-induced detachment performances	57:110	antibacterial and EDTA-induced detachment performances	57:110	Berberine carried gelatin/sodium alginate hydrogels with antibacterial and EDTA-induced detachment performances.					
33892030	9	66	theme	anti-bacterial	983:996	arg1	effect					998:1003	anti-bacterial effect	983:1003	anti-bacterial effect against Staphylococcus aureus	983:1033	The BBR/G/SA hydrogels show sustained drug release for 168 h and exhibit anti-bacterial effect against Staphylococcus aureus.					
32682959	0	0	theme	antibacterial	90:102	arg1	properties					104:113	antibacterial properties	90:113	antibacterial properties	90:113	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	7	1	contain	possess	1192:1198	arg1	NPs					1188:1190	the obtained NPs	1175:1190	the obtained NPs	1175:1190	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	1	contain	possess	1192:1198	arg2	phases					1206:1211	mixed phases	1200:1211	mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM))	1200:1348	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	9	2	theme	NP	1508:1509	arg1	samples					1511:1517	the NP samples	1504:1517	the NP samples	1504:1517	It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli.					
32682959	7	3	theme	obtained	1179:1186	arg1	NPs					1188:1190	the obtained NPs	1175:1190	the obtained NPs	1175:1190	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	13	4	from	findings	2076:2083	arg1	paper					2093:2097	this paper	2088:2097	this paper	2088:2097	Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.					
32682959	10	5	theme	Candida	1750:1756	arg1	albicans					1758:1765	Candida albicans	1750:1765	Candida albicans	1750:1765	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	12	6	theme	20	2012:2013	arg1	drug					1971:1974	released drug	1962:1974	released drug from the tested NPs	1962:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	6	theme	20	2012:2013	arg1	amounts					1951:1957	the amounts	1947:1957	the amounts of released drug from the tested NPs	1947:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	6	theme	20	2012:2013	arg1	amount					2041:2046	the originally loaded amount	2019:2046	the originally loaded amount	2019:2046	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	6	theme	20	2012:2013	arg1	%					2014:2014	85, 26 and 20%	2001:2014	%	2014:2014	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	9	7	theme	Cu	1493:1494	arg1	ions					1496:1499	Cu ions	1493:1499	Cu ions	1493:1499	It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli.					
32682959	4	8	theme	particle	815:822	arg1	morphology					824:833	particle morphology	815:833	particle morphology	815:833	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	8	theme	particle	815:822	arg1	NPs					765:767	the prepared NPs	752:767	the prepared NPs	752:767	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	11	9	theme	higher	1832:1837	arg1	concentration					1846:1858	the higher Cu ion concentration	1828:1858	the higher Cu ion concentration	1828:1858	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	13	10	theme	good	2141:2144	arg1	applications					2174:2185	good potential for drug delivery applications	2141:2185	good potential for drug delivery applications	2141:2185	Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.					
32682959	5	11	theme	Antibacterial	899:911	arg1	properties					913:922	Antibacterial properties	899:922	Antibacterial properties of the NPs	899:933	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	4	12	theme	chemical	877:884	arg1	composition					886:896	chemical composition	877:896	chemical composition	877:896	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	12	theme	chemical	877:884	arg1	NPs					765:767	the prepared NPs	752:767	the prepared NPs	752:767	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	0	13	theme	release	124:130	arg1	kinetics					132:139	drug release kinetics	119:139	drug release kinetics	119:139	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	5	14	theme	Candida	1043:1049	arg1	albicans					1051:1058	Candida albicans	1043:1058	Candida albicans (yeast)	1043:1066	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	5	14	theme	Candida	1043:1049	arg1	yeast					1061:1065	yeast	1061:1065	yeast	1061:1065	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	8	15	theme	magnetization	1362:1374	arg1	38.7					1411:1414	38.7	1411:1414	38.7	1411:1414	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	15	theme	magnetization	1362:1374	arg1	values					1376:1381	Saturation magnetization values	1351:1381	Saturation magnetization values of the NPs	1351:1392	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	15	theme	magnetization	1362:1374	arg1	1.3 emu/g					1425:1433	1.3 emu/g	1425:1433	1.3 emu/g	1425:1433	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	15	theme	magnetization	1362:1374	arg1	3.5					1417:1419	3.5	1417:1419	3.5	1417:1419	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	4	16	theme	various	730:736	arg1	properties					738:747	various properties	730:747	various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition	730:896	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	7	17	theme	Cu	1276:1277	arg1	substitution					1283:1294	Cu ion substitution	1276:1294	Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM))	1276:1348	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	13	18	theme	drug	2160:2163	arg1	delivery					2165:2172	drug delivery	2160:2172	drug delivery	2160:2172	Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.					
32682959	0	19	theme	drug	119:122	arg1	kinetics					132:139	drug release kinetics	119:139	drug release kinetics	119:139	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	1	20	with	nanoparticles	158:170	arg1	M					193:193	M	193:193	M	193:193	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	1	20	with	nanoparticles	158:170	arg1	magnetic					183:190	magnetic	183:190	magnetic	183:190	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	1	20	with	nanoparticles	158:170	arg1	properties					214:223	antibacterial properties	200:223	antibacterial properties	200:223	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	5	21	theme	NPs	931:933	arg1	properties					913:922	Antibacterial properties	899:922	Antibacterial properties of the NPs	899:933	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	10	22	contain	have	1656:1659	arg1	ciprofloxacin					1628:1640	ciprofloxacin	1628:1640	ciprofloxacin	1628:1640	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	10	22	contain	have	1656:1659	arg2	effects					1670:1676	stronger effects	1661:1676	stronger effects against Bacillus cereus and Escherichia coli	1661:1721	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	10	22	contain	have	1656:1659	arg1	Chitosan					1615:1622	Chitosan	1615:1622	Chitosan	1615:1622	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	10	22	contain	have	1656:1659	arg2	effects					1734:1740	lesser effects	1727:1740	lesser effects against Candida albicans	1727:1765	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	0	23	theme	magnetite	16:24	arg1	nanoparticles					26:38	Multifunctional magnetite nanoparticles	0:38	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.	0:140	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	23	theme	magnetite	16:24	arg1	characterisation					72:87	characterisation	72:87	characterisation	72:87	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	23	theme	magnetite	16:24	arg1	Preparation					59:69	Preparation	59:69	Preparation	59:69	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	12	24	from	NPs	1992:1994	arg1	amount					2041:2046	the originally loaded amount	2019:2046	the originally loaded amount	2019:2046	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	24	from	NPs	1992:1994	arg1	amounts					1951:1957	the amounts	1947:1957	the amounts of released drug from the tested NPs	1947:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	24	from	NPs	1992:1994	arg1	drug					1971:1974	released drug	1962:1974	released drug from the tested NPs	1962:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	24	from	NPs	1992:1994	arg1	%					2014:2014	85, 26 and 20%	2001:2014	%	2014:2014	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	6	25	theme	release	1101:1107	arg1	Efficiency					1069:1078	Efficiency	1069:1078	Efficiency of the ciprofloxacin release	1069:1107	Efficiency of the ciprofloxacin release was also studied for the drug-loaded NPs.					
32682959	0	26	theme	Multifunctional	0:14	arg1	nanoparticles					26:38	Multifunctional magnetite nanoparticles	0:38	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.	0:140	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	26	theme	Multifunctional	0:14	arg1	characterisation					72:87	characterisation	72:87	characterisation	72:87	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	26	theme	Multifunctional	0:14	arg1	Preparation					59:69	Preparation	59:69	Preparation	59:69	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	6	27	theme	drug-loaded	1134:1144	arg1	NPs					1146:1148	the drug-loaded NPs	1130:1148	the drug-loaded NPs	1130:1148	Efficiency of the ciprofloxacin release was also studied for the drug-loaded NPs.					
32682959	11	28	contain	containing	1789:1798	arg2	concentration					1846:1858	the higher Cu ion concentration	1828:1858	the higher Cu ion concentration	1828:1858	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	11	28	contain	containing	1789:1798	arg2	ciprofloxacin					1810:1822	ciprofloxacin	1810:1822	ciprofloxacin	1810:1822	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	11	28	contain	containing	1789:1798	arg2	chitosan					1800:1807	chitosan	1800:1807	chitosan	1800:1807	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	11	28	contain	containing	1789:1798	arg1	samples					1781:1787	the samples	1777:1787	the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration	1777:1858	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	13	29	theme	developed	2104:2112	arg1	NPs					2114:2116	the developed NPs	2100:2116	the developed NPs	2100:2116	Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.					
32682959	2	30	theme	complete	328:335	arg1	substitutions					337:349	Partial and complete substitutions	316:349	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+)	316:394	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	12	31	theme	amount	2041:2046	arg1	drug					1971:1974	released drug	1962:1974	released drug from the tested NPs	1962:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	31	theme	amount	2041:2046	arg1	amounts					1951:1957	the amounts	1947:1957	the amounts of released drug from the tested NPs	1947:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	31	theme	amount	2041:2046	arg1	amount					2041:2046	the originally loaded amount	2019:2046	the originally loaded amount	2019:2046	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	31	theme	amount	2041:2046	arg1	%					2014:2014	85, 26 and 20%	2001:2014	%	2014:2014	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	1	32	theme	co-precipitation	288:303	arg1	synthesis					305:313	co-precipitation synthesis	288:313	co-precipitation synthesis	288:313	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	4	33	theme	magnetic	855:862	arg1	strength					864:871	magnetic strength	855:871	magnetic strength	855:871	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	33	theme	magnetic	855:862	arg1	NPs					765:767	the prepared NPs	752:767	the prepared NPs	752:767	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	11	34	theme	Cu	1839:1840	arg1	concentration					1846:1858	the higher Cu ion concentration	1828:1858	the higher Cu ion concentration	1828:1858	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	9	35	from	introduction	1477:1488	arg1	samples					1511:1517	the NP samples	1504:1517	the NP samples	1504:1517	It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli.					
32682959	12	36	theme	tested	1985:1990	arg1	NPs					1992:1994	the tested NPs	1981:1994	the tested NPs	1981:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	6	37	theme	ciprofloxacin	1087:1099	arg1	release					1101:1107	the ciprofloxacin release	1083:1107	the ciprofloxacin release	1083:1107	Efficiency of the ciprofloxacin release was also studied for the drug-loaded NPs.					
32682959	9	38	theme	activity	1568:1575	arg1	significance					1532:1543	the significance	1528:1543	the significance of their antibacterial activity	1528:1575	It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli.					
32682959	3	39	theme	previous	601:608	arg1	step					610:613	the previous step	597:613	the previous step to achieve multifunctional properties	597:651	In addition, chitosan and ciprofloxacin were hybridized with the NPs from the previous step to achieve multifunctional properties.					
32682959	2	40	theme	Partial	316:322	arg1	substitutions					337:349	Partial and complete substitutions	316:349	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+)	316:394	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	13	41	theme	pre-clinical	2224:2235	arg1	studies					2237:2243	pre-clinical studies	2224:2243	pre-clinical studies	2224:2243	Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.					
32682959	1	42	theme	antibacterial	200:212	arg1	properties					214:223	antibacterial properties	200:223	antibacterial properties	200:223	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	7	43	theme	crystalline	1218:1228	arg1	structures					1230:1239	crystalline structures	1218:1239	crystalline structures	1218:1239	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	13	44	theme	potential	2146:2154	arg1	applications					2174:2185	good potential for drug delivery applications	2141:2185	good potential for drug delivery applications	2141:2185	Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.					
32682959	5	45	theme	Gram-negative	1015:1027	arg1	coli					1009:1012	Escherichia coli	997:1012	Escherichia coli (Gram-negative bacteria)	997:1037	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	5	45	theme	Gram-negative	1015:1027	arg1	bacteria					1029:1036	Gram-negative bacteria	1015:1036	Gram-negative bacteria	1015:1036	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	11	46	theme	ion	1842:1844	arg1	concentration					1846:1858	the higher Cu ion concentration	1828:1858	the higher Cu ion concentration	1828:1858	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	7	47	dep	substitution	1283:1294	arg1	CuM					1344:1346	CuM	1344:1346	CuM	1344:1346	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	47	dep	substitution	1283:1294	arg1	Cu0.5M					1322:1327	Cu0.5M	1322:1327	Cu0.5M	1322:1327	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	47	dep	substitution	1283:1294	arg1	5-10 nm					1297:1303	5-10 nm	1297:1303	5-10 nm (M)	1297:1307	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	47	dep	substitution	1283:1294	arg1	M					1306:1306	M	1306:1306	M	1306:1306	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	47	dep	substitution	1283:1294	arg1	11-16 nm					1334:1341	11-16 nm	1334:1341	11-16 nm (CuM)	1334:1347	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	47	dep	substitution	1283:1294	arg1	2.5-3.5 nm					1310:1319	2.5-3.5 nm	1310:1319	2.5-3.5 nm (Cu0.5M)	1310:1328	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	0	48	theme	drug	44:47	arg1	delivery					49:56	drug delivery	44:56	drug delivery	44:56	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	11	49	theme	Candida	1895:1901	arg1	albicans					1903:1910	Candida albicans	1895:1910	Candida albicans	1895:1910	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	4	50	theme	NPs	765:767	arg1	properties					738:747	various properties	730:747	various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition	730:896	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	9	51	theme	ions	1496:1499	arg1	introduction					1477:1488	the introduction	1473:1488	the introduction of Cu ions in the NP samples	1473:1517	It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli.					
32682959	3	52	from	step	610:613	arg1	NPs					588:590	the NPs	584:590	the NPs from the previous step to achieve multifunctional properties	584:651	In addition, chitosan and ciprofloxacin were hybridized with the NPs from the previous step to achieve multifunctional properties.					
32682959	2	53	theme	NPs	450:452	arg1	preparation					421:431	the preparation	417:431	the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work	417:520	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	10	54	theme	lesser	1727:1732	arg1	effects					1734:1740	lesser effects	1727:1740	lesser effects against Candida albicans	1727:1765	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	5	55	theme	Gram-positive	972:984	arg1	cereus					964:969	Bacillus cereus	955:969	Bacillus cereus (Gram-positive bacteria)	955:994	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	5	55	theme	Gram-positive	972:984	arg1	bacteria					986:993	Gram-positive bacteria	972:993	Gram-positive bacteria	972:993	Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast).					
32682959	12	56	theme	released	1962:1969	arg1	drug					1971:1974	released drug	1962:1974	released drug from the tested NPs	1962:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	2	57	theme	magnetite	440:448	arg1	NPs					450:452	the magnetite NPs	436:452	the magnetite NPs	436:452	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	2	57	theme	magnetite	440:448	arg1	Cu0.5M					479:484	Cu0.5M	479:484	Cu0.5M	479:484	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	2	57	theme	magnetite	440:448	arg1	CuM					490:492	CuM	490:492	CuM	490:492	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	9	58	theme	antibacterial	1554:1566	arg1	activity					1568:1575	their antibacterial activity	1548:1575	their antibacterial activity	1548:1575	It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli.					
32682959	3	59	theme	multifunctional	626:640	arg1	properties					642:651	multifunctional properties	626:651	multifunctional properties	626:651	In addition, chitosan and ciprofloxacin were hybridized with the NPs from the previous step to achieve multifunctional properties.					
32682959	7	60	with	phases	1206:1211	arg1	structures					1230:1239	crystalline structures	1218:1239	crystalline structures	1218:1239	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	2	61	theme	ions	362:365	arg1	substitutions					337:349	Partial and complete substitutions	316:349	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+)	316:394	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	11	62	theme	strong	1870:1875	arg1	influence					1877:1885	strong influence	1870:1885	strong influence	1870:1885	However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans.					
32682959	8	63	theme	Saturation	1351:1360	arg1	38.7					1411:1414	38.7	1411:1414	38.7	1411:1414	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	63	theme	Saturation	1351:1360	arg1	values					1376:1381	Saturation magnetization values	1351:1381	Saturation magnetization values of the NPs	1351:1392	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	63	theme	Saturation	1351:1360	arg1	1.3 emu/g					1425:1433	1.3 emu/g	1425:1433	1.3 emu/g	1425:1433	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	63	theme	Saturation	1351:1360	arg1	3.5					1417:1419	3.5	1417:1419	3.5	1417:1419	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	7	64	theme	substitution	1283:1294	arg1	degree					1266:1271	the degree	1262:1271	the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM))	1262:1348	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	1	65	theme	Multifunctional	142:156	arg1	NPs					173:175	NPs	173:175	NPs	173:175	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	1	65	theme	Multifunctional	142:156	arg1	nanoparticles					158:170	Multifunctional nanoparticles	142:170	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties	142:223	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	4	66	theme	elemental	836:844	arg1	mapping					846:852	elemental mapping	836:852	elemental mapping	836:852	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	66	theme	elemental	836:844	arg1	NPs					765:767	the prepared NPs	752:767	the prepared NPs	752:767	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	0	67	dep	nanoparticles	26:38	arg1	nanoparticles					26:38	Multifunctional magnetite nanoparticles	0:38	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.	0:140	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	67	dep	nanoparticles	26:38	arg1	kinetics					132:139	drug release kinetics	119:139	drug release kinetics	119:139	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	67	dep	nanoparticles	26:38	arg1	characterisation					72:87	characterisation	72:87	characterisation	72:87	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	67	dep	nanoparticles	26:38	arg1	properties					104:113	antibacterial properties	90:113	antibacterial properties	90:113	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	0	67	dep	nanoparticles	26:38	arg1	Preparation					59:69	Preparation	59:69	Preparation	59:69	Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics.					
32682959	12	68	from	amounts	1951:1957	arg1	NPs					1992:1994	the tested NPs	1981:1994	the tested NPs	1981:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	69	theme	drug	1971:1974	arg1	amount					2041:2046	the originally loaded amount	2019:2046	the originally loaded amount	2019:2046	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	69	theme	drug	1971:1974	arg1	amounts					1951:1957	the amounts	1947:1957	the amounts of released drug from the tested NPs	1947:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	69	theme	drug	1971:1974	arg1	drug					1971:1974	released drug	1962:1974	released drug from the tested NPs	1962:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	69	theme	drug	1971:1974	arg1	%					2014:2014	85, 26 and 20%	2001:2014	%	2014:2014	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	10	70	theme	stronger	1661:1668	arg1	effects					1670:1676	stronger effects	1661:1676	stronger effects against Bacillus cereus and Escherichia coli	1661:1721	Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans.					
32682959	2	71	theme	ferrous	354:360	arg1	ions					362:365	ferrous ions	354:365	ferrous ions (Fe2+)	354:372	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	2	71	theme	ferrous	354:360	arg1	Fe2+					368:371	Fe2+	368:371	Fe2+	368:371	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	12	72	theme	study	1922:1926	arg1	period					1928:1933	a study period	1920:1933	a study period of 30-days	1920:1944	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	4	73	theme	prepared	756:763	arg1	crystallinity					778:790	crystallinity	778:790	crystallinity	778:790	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	73	theme	prepared	756:763	arg1	strength					864:871	magnetic strength	855:871	magnetic strength	855:871	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	73	theme	prepared	756:763	arg1	nanostructure					793:805	nanostructure	793:805	nanostructure (size)	793:812	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	73	theme	prepared	756:763	arg1	morphology					824:833	particle morphology	815:833	particle morphology	815:833	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	73	theme	prepared	756:763	arg1	NPs					765:767	the prepared NPs	752:767	the prepared NPs	752:767	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	73	theme	prepared	756:763	arg1	mapping					846:852	elemental mapping	836:852	elemental mapping	836:852	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	4	73	theme	prepared	756:763	arg1	composition					886:896	chemical composition	877:896	chemical composition	877:896	XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition.					
32682959	2	74	theme	copper	377:382	arg1	Cu2+					390:393	Cu2+	390:393	Cu2+	390:393	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	2	74	theme	copper	377:382	arg1	ions					384:387	copper ions	377:387	copper ions (Cu2+)	377:394	Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work.					
32682959	12	75	theme	85	2001:2002	arg1	drug					1971:1974	released drug	1962:1974	released drug from the tested NPs	1962:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	75	theme	85	2001:2002	arg1	amounts					1951:1957	the amounts	1947:1957	the amounts of released drug from the tested NPs	1947:1994	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	75	theme	85	2001:2002	arg1	amount					2041:2046	the originally loaded amount	2019:2046	the originally loaded amount	2019:2046	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	75	theme	85	2001:2002	arg1	%					2014:2014	85, 26 and 20%	2001:2014	%	2014:2014	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	1	76	theme	drug	243:246	arg1	purposes					257:264	drug delivery purposes	243:264	drug delivery purposes	243:264	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
32682959	8	77	theme	NPs	1390:1392	arg1	38.7					1411:1414	38.7	1411:1414	38.7	1411:1414	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	77	theme	NPs	1390:1392	arg1	values					1376:1381	Saturation magnetization values	1351:1381	Saturation magnetization values of the NPs	1351:1392	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	77	theme	NPs	1390:1392	arg1	1.3 emu/g					1425:1433	1.3 emu/g	1425:1433	1.3 emu/g	1425:1433	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	8	77	theme	NPs	1390:1392	arg1	3.5					1417:1419	3.5	1417:1419	3.5	1417:1419	Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively.					
32682959	7	78	theme	ion	1279:1281	arg1	substitution					1283:1294	Cu ion substitution	1276:1294	Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM))	1276:1348	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	7	79	theme	mixed	1200:1204	arg1	phases					1206:1211	mixed phases	1200:1211	mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM))	1200:1348	It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10 nm (M), 2.5-3.5 nm (Cu0.5M) and 11-16 nm (CuM)).					
32682959	12	80	theme	30-days	1938:1944	arg1	period					1928:1933	a study period	1920:1933	a study period of 30-days	1920:1944	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	12	81	theme	loaded	2034:2039	arg1	amount					2041:2046	the originally loaded amount	2019:2046	the originally loaded amount	2019:2046	During a study period of 30-days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively.					
32682959	1	82	theme	delivery	248:255	arg1	purposes					257:264	drug delivery purposes	243:264	drug delivery purposes	243:264	Multifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis.					
33691298	1	0	theme	3D	170:171	arg1	printing					173:180	3D printing	170:180	3D printing	170:180	The aim of this study was to obtain hierarchical scaffolds combining 3D printing and two electrofluidodynamic methods.					
33691298	3	1	theme	electrospraying	513:527	arg1	process					561:567	the electrospraying (electro dynamic spraying, EDS) process	509:567	the electrospraying (electro dynamic spraying, EDS) process	509:567	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	9	2	theme	tissue	1508:1513	arg1	engineering					1515:1525	tissue engineering	1508:1525	tissue engineering	1508:1525	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	6	3	theme	biological	920:929	arg1	tests					931:935	Preliminary biological tests	908:935	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1)	908:972	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	4	4	theme	flow	762:765	arg1	rate					767:770	solution flow rate	753:770	solution flow rate	753:770	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	3	5	used	used	482:485	arg2	CHLA					468:471	CHLA	468:471	CHLA	468:471	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	9	6	theme	CHLA	1432:1435	arg1	value					1423:1427	the added value	1413:1427	the added value of CHLA	1413:1435	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	6	7	theme	Preliminary	908:918	arg1	tests					931:935	Preliminary biological tests	908:935	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1)	908:972	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	4	8	theme	solution	753:760	arg1	rate					767:770	solution flow rate	753:770	solution flow rate	753:770	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	7	9	theme	printed	1167:1173	arg1	struts					1175:1180	the 3D printed struts	1160:1180	the 3D printed struts	1160:1180	Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding.					
33691298	2	10	theme	modified	392:399	arg1	chitosan					401:408	hydrophobically modified chitosan	376:408	hydrophobically modified chitosan	376:408	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	2	10	theme	modified	392:399	arg1	chitosan					418:425	chitosan	418:425	chitosan grafted with linoleic acid (CHLA)	418:459	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	1	11	theme	hierarchical	137:148	arg1	scaffolds					150:158	hierarchical scaffolds	137:158	hierarchical scaffolds combining 3D printing and two electrofluidodynamic methods	137:217	The aim of this study was to obtain hierarchical scaffolds combining 3D printing and two electrofluidodynamic methods.					
33691298	8	12	theme	nanoparticles	1306:1318	arg1	addition					1275:1282	the addition	1271:1282	the addition of the electrosprayed nanoparticles	1271:1318	Moreover, the addition of the electrosprayed nanoparticles does not affect the cell seeding efficiency.					
33691298	6	13	from	effect	1008:1013	arg1	efficacy					1060:1067	cell seeding efficacy	1047:1067	cell seeding efficacy	1047:1067	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	5	14	theme	electrospraying	796:810	arg1	conditions					812:821	the optimized electrospraying conditions	782:821	the optimized electrospraying conditions	782:821	By using the optimized electrospraying conditions, uniformly distributed spheres have been obtained, decorating struts and nanofibers.					
33691298	3	15	theme	dynamic	538:544	arg1	EDS					556:558	EDS	556:558	EDS	556:558	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	3	15	theme	dynamic	538:544	arg1	spraying					546:553	electro dynamic spraying	530:553	electro dynamic spraying	530:553	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	7	16	theme	cell	1126:1129	arg1	respect					1149:1155	respect	1149:1155	respect to the 3D printed struts alone	1149:1186	Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding.					
33691298	7	16	theme	cell	1126:1129	arg1	efficacy					1139:1146	cell seeding efficacy	1126:1146	cell seeding efficacy	1126:1146	Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding.					
33691298	7	17	theme	seeding	1131:1137	arg1	respect					1149:1155	respect	1149:1155	respect to the 3D printed struts alone	1149:1186	Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding.					
33691298	7	17	theme	seeding	1131:1137	arg1	efficacy					1139:1146	cell seeding efficacy	1126:1146	cell seeding efficacy	1126:1146	Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding.					
33691298	9	18	theme	related	1535:1541	arg1	applications					1492:1503	several applications	1484:1503	several applications	1484:1503	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	0	19	theme	multi-layered	13:25	arg1	scaffolds					27:35	Hierarchical multi-layered scaffolds	0:35	Hierarchical multi-layered scaffolds	0:35	Hierarchical multi-layered scaffolds based on electrofluidodynamic processes for tissue engineering.					
33691298	9	20	with	versatility	1369:1379	arg1	value					1423:1427	the added value	1413:1427	the added value of CHLA	1413:1435	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	4	21	theme	CHLA	732:735	arg1	dissolution					737:747	CHLA dissolution	732:747	CHLA dissolution	732:747	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	1	22	theme	electrofluidodynamic	190:209	arg1	methods					211:217	two electrofluidodynamic methods	186:217	two electrofluidodynamic methods	186:217	The aim of this study was to obtain hierarchical scaffolds combining 3D printing and two electrofluidodynamic methods.					
33691298	0	23	theme	Hierarchical	0:11	arg1	scaffolds					27:35	Hierarchical multi-layered scaffolds	0:35	Hierarchical multi-layered scaffolds	0:35	Hierarchical multi-layered scaffolds based on electrofluidodynamic processes for tissue engineering.					
33691298	4	24	theme	acid	718:721	arg1	composition					688:698	the solvent mixture composition	668:698	the solvent mixture composition	668:698	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	4	24	theme	acid	718:721	arg1	concentration					701:713	concentration	701:713	concentration of acid used for CHLA dissolution and solution flow rate	701:770	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	3	25	from	time	501:504	arg1	process					561:567	the electrospraying (electro dynamic spraying, EDS) process	509:567	the electrospraying (electro dynamic spraying, EDS) process	509:567	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	3	26	dep	electrospraying	513:527	arg1	EDS					556:558	EDS	556:558	EDS	556:558	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	3	26	dep	electrospraying	513:527	arg1	spraying					546:553	electro dynamic spraying	530:553	electro dynamic spraying	530:553	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	9	27	theme	added	1417:1421	arg1	value					1423:1427	the added value	1413:1427	the added value of CHLA	1413:1435	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	8	28	theme	cell	1340:1343	arg1	efficiency					1353:1362	the cell seeding efficiency	1336:1362	the cell seeding efficiency	1336:1362	Moreover, the addition of the electrosprayed nanoparticles does not affect the cell seeding efficiency.					
33691298	2	29	theme	3D	262:263	arg1	struts					273:278	3D printed struts	262:278	3D printed struts	262:278	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	4	30	theme	EDS	631:633	arg1	process					635:641	The EDS process	627:641	The EDS process	627:641	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	7	31	theme	hierarchical	1094:1105	arg1	structure					1107:1115	the hierarchical structure	1090:1115	the hierarchical structure	1090:1115	Results showed that the hierarchical structure enhances cell seeding efficacy, respect to the 3D printed struts alone, preventing that the cells passed through the struts during the seeding.					
33691298	1	32	theme	study	117:121	arg1	aim					105:107	The aim	101:107	The aim of this study	101:121	The aim of this study was to obtain hierarchical scaffolds combining 3D printing and two electrofluidodynamic methods.					
33691298	9	33	theme	delivery	1551:1558	arg1	systems					1560:1566	drug delivery systems	1546:1566	drug delivery systems	1546:1566	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	9	34	theme	several	1484:1490	arg1	applications					1492:1503	several applications	1484:1503	several applications	1484:1503	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	0	35	theme	electrofluidodynamic	46:65	arg1	processes					67:75	electrofluidodynamic processes	46:75	electrofluidodynamic processes for tissue engineering	46:98	Hierarchical multi-layered scaffolds based on electrofluidodynamic processes for tissue engineering.					
33691298	3	36	theme	spheres	587:593	arg1	formation					574:582	the formation	570:582	the formation of spheres	570:593	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	9	37	theme	drug	1546:1549	arg1	systems					1560:1566	drug delivery systems	1546:1566	drug delivery systems	1546:1566	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	9	38	theme	structure	1397:1405	arg1	versatility					1369:1379	The versatility	1365:1379	The versatility	1365:1379	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	9	39	from	applications	1492:1503	arg1	engineering					1515:1525	tissue engineering	1508:1525	tissue engineering	1508:1525	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	2	40	theme	linoleic	440:447	arg1	acid					449:452	linoleic acid	440:452	linoleic acid (CHLA)	440:459	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	2	40	theme	linoleic	440:447	arg1	CHLA					455:458	CHLA	455:458	CHLA	455:458	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	6	41	with	tests	931:935	arg1	MC3T3-E1					964:971	MC3T3-E1	964:971	MC3T3-E1	964:971	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	6	41	with	tests	931:935	arg1	preosteoblasts					948:961	mouse preosteoblasts	942:961	mouse preosteoblasts (MC3T3-E1)	942:972	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	5	42	theme	optimized	786:794	arg1	conditions					812:821	the optimized electrospraying conditions	782:821	the optimized electrospraying conditions	782:821	By using the optimized electrospraying conditions, uniformly distributed spheres have been obtained, decorating struts and nanofibers.					
33691298	9	43	theme	proposed	1388:1395	arg1	structure					1397:1405	the proposed structure	1384:1405	the proposed structure	1384:1405	The versatility of the proposed structure, with the added value of CHLA nanoparticles decoration could be suitable for several applications in tissue engineering, mainly related to drug delivery systems.					
33691298	8	44	theme	electrosprayed	1291:1304	arg1	nanoparticles					1306:1318	the electrosprayed nanoparticles	1287:1318	the electrosprayed nanoparticles	1287:1318	Moreover, the addition of the electrosprayed nanoparticles does not affect the cell seeding efficiency.					
33691298	2	45	theme	printed	265:271	arg1	struts					273:278	3D printed struts	262:278	3D printed struts	262:278	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	2	46	theme	multi-layered	224:236	arg1	scaffold					238:245	The multi-layered scaffold	220:245	The multi-layered scaffold	220:245	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	2	47	theme	electrospun	281:291	arg1	fibers					293:298	electrospun fibers	281:298	electrospun fibers obtained from poly(ϵ-caprolactone)	281:333	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	4	48	theme	solvent	672:678	arg1	composition					688:698	the solvent mixture composition	668:698	the solvent mixture composition	668:698	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	4	48	theme	solvent	672:678	arg1	concentration					701:713	concentration	701:713	concentration of acid used for CHLA dissolution and solution flow rate	701:770	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	6	49	theme	cell	1047:1050	arg1	efficacy					1060:1067	cell seeding efficacy	1047:1067	cell seeding efficacy	1047:1067	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	2	50	theme	electrosprayed	339:352	arg1	spheres					354:360	electrosprayed spheres	339:360	electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA)	339:459	The multi-layered scaffold is composed by 3D printed struts, electrospun fibers obtained from poly(ϵ-caprolactone) and electrosprayed spheres produced from hydrophobically modified chitosan, namely chitosan grafted with linoleic acid (CHLA).					
33691298	8	51	theme	seeding	1345:1351	arg1	efficiency					1353:1362	the cell seeding efficiency	1336:1362	the cell seeding efficiency	1336:1362	Moreover, the addition of the electrosprayed nanoparticles does not affect the cell seeding efficiency.					
33691298	6	52	theme	seeding	1052:1058	arg1	efficacy					1060:1067	cell seeding efficacy	1047:1067	cell seeding efficacy	1047:1067	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	5	53	theme	distributed	834:844	arg1	spheres					846:852	uniformly distributed spheres	824:852	uniformly distributed spheres	824:852	By using the optimized electrospraying conditions, uniformly distributed spheres have been obtained, decorating struts and nanofibers.					
33691298	4	54	theme	mixture	680:686	arg1	composition					688:698	the solvent mixture composition	668:698	the solvent mixture composition	668:698	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	4	54	theme	mixture	680:686	arg1	concentration					701:713	concentration	701:713	concentration of acid used for CHLA dissolution and solution flow rate	701:770	The EDS process was strongly affected by the solvent mixture composition, concentration of acid used for CHLA dissolution and solution flow rate.					
33691298	3	55	theme	first	495:499	arg1	time					501:504	the first time	491:504	the first time in the electrospraying (electro dynamic spraying, EDS) process	491:567	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	6	56	theme	scaffold	1035:1042	arg1	effect					1008:1013	the effect	1004:1013	the effect of the hierarchical scaffold on cell seeding efficacy	1004:1067	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	0	57	theme	tissue	81:86	arg1	engineering					88:98	tissue engineering	81:98	tissue engineering	81:98	Hierarchical multi-layered scaffolds based on electrofluidodynamic processes for tissue engineering.					
33691298	6	58	theme	mouse	942:946	arg1	MC3T3-E1					964:971	MC3T3-E1	964:971	MC3T3-E1	964:971	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	6	58	theme	mouse	942:946	arg1	preosteoblasts					948:961	mouse preosteoblasts	942:961	mouse preosteoblasts (MC3T3-E1)	942:972	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33691298	3	59	theme	optimization	605:616	arg1	process					618:624	an optimization process	602:624	an optimization process	602:624	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	3	60	theme	electro	530:536	arg1	EDS					556:558	EDS	556:558	EDS	556:558	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	3	60	theme	electro	530:536	arg1	spraying					546:553	electro dynamic spraying	530:553	electro dynamic spraying	530:553	Since CHLA has been used for the first time in the electrospraying (electro dynamic spraying, EDS) process, the formation of spheres needed an optimization process.					
33691298	6	61	theme	hierarchical	1022:1033	arg1	scaffold					1035:1042	the hierarchical scaffold	1018:1042	the hierarchical scaffold	1018:1042	Preliminary biological tests with mouse preosteoblasts (MC3T3-E1) were performed to investigate the effect of the hierarchical scaffold on cell seeding efficacy.					
33877245	7	0	theme	responder	1076:1084	arg1	group					1086:1090	responder group	1076:1090	responder group	1076:1090	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	0	1	from	fingerprint	22:32	arg1	subjects					37:44	subjects	37:44	subjects with irritable bowel syndrome	37:74	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	9	2	theme	accuracy	1491:1498	arg1	model					1500:1504	LDA accuracy model	1487:1504	LDA accuracy model	1487:1504	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	0	3	with	subjects	37:44	arg1	syndrome					67:74	irritable bowel syndrome	51:74	irritable bowel syndrome	51:74	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	5	4	theme	Analogue	793:800	arg1	Scale					802:806	Visual Analogue Scale	786:806	Visual Analogue Scale	786:806	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	5	4	theme	Analogue	793:800	arg1	LFD					781:783	a four-week LFD	769:783	a four-week LFD	769:783	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	9	5	theme	responder	1553:1561	arg1	group					1563:1567	responder group	1553:1567	responder group	1553:1567	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	9	6	theme	non-responder	1582:1594	arg1	group					1596:1600	100% and non-responder group	1573:1600	100% and non-responder group	1573:1600	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	7	7	theme	Variant	999:1005	arg1	analysis					969:976	Differential abundance analysis	946:976	Differential abundance analysis	946:976	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	9	8	theme	Linear	1348:1353	arg1	Analysis					1368:1375	Linear Discriminant Analysis	1348:1375	Linear Discriminant Analysis	1348:1375	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	9	8	theme	Linear	1348:1353	arg1	LDA					1378:1380	LDA	1378:1380	LDA	1378:1380	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	0	9	theme	FODMAP	96:101	arg1	diet					103:106	a low FODMAP diet	90:106	a low FODMAP diet	90:106	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	9	10	theme	genera	1479:1484	arg1	abundance					1454:1462	baseline abundance	1445:1462	baseline abundance of 5 bacterial genera	1445:1484	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	7	11	theme	Sequence	990:997	arg1	Variant					999:1005	Amplicon Sequence Variant	981:1005	Amplicon Sequence Variant (ASVs)	981:1012	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	7	11	theme	Sequence	990:997	arg1	ASVs					1008:1011	ASVs	1008:1011	ASVs	1008:1011	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	5	12	theme	Visual	786:791	arg1	Scale					802:806	Visual Analogue Scale	786:806	Visual Analogue Scale	786:806	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	5	12	theme	Visual	786:791	arg1	LFD					781:783	a four-week LFD	769:783	a four-week LFD	769:783	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	10	13	from	conclusion	1606:1615	arg1	useful					1643:1648	useful	1643:1648	useful	1643:1648	In conclusion, bacterial biomarkers are useful to classify IBS individuals by responsiveness to LFD.					
33877245	0	14	theme	low	92:94	arg1	diet					103:106	a low FODMAP diet	90:106	a low FODMAP diet	90:106	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	2	15	theme	Fermentable	272:282	arg1	Oligosaccharides					284:299	Fermentable Oligosaccharides	272:299	Fermentable Oligosaccharides	272:299	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	2	16	dep	diet	347:350	arg1	Polyols					338:344	Polyols	338:344	Polyols	338:344	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	2	16	dep	diet	347:350	arg1	Disaccharides					302:314	Disaccharides	302:314	Disaccharides	302:314	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	2	16	dep	diet	347:350	arg1	Oligosaccharides					284:299	Fermentable Oligosaccharides	272:299	Fermentable Oligosaccharides	272:299	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	2	16	dep	diet	347:350	arg1	Monosaccharides					317:331	Monosaccharides	317:331	Monosaccharides	317:331	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	1	17	theme	Irritable	109:117	arg1	Syndrome					125:132	Irritable Bowel Syndrome	109:132	Irritable Bowel Syndrome (IBS)	109:138	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	1	17	theme	Irritable	109:117	arg1	disorder					173:180	a functional gastrointestinal disorder	143:180	a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit	143:236	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	1	17	theme	Irritable	109:117	arg1	IBS					135:137	IBS	135:137	IBS	135:137	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	4	18	theme	fecal	583:587	arg1	composition					599:609	baseline fecal microbial composition	574:609	baseline fecal microbial composition	574:609	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	4	19	theme	rRNA	631:634	arg1	region					647:652	the 16S rRNA gene V3-V4 region	623:652	the 16S rRNA gene V3-V4 region	623:652	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	1	20	theme	functional	145:154	arg1	disorder					173:180	a functional gastrointestinal disorder	143:180	a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit	143:236	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	1	20	theme	functional	145:154	arg1	Syndrome					125:132	Irritable Bowel Syndrome	109:132	Irritable Bowel Syndrome (IBS)	109:138	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	7	21	theme	Amplicon	981:988	arg1	Variant					999:1005	Amplicon Sequence Variant	981:1005	Amplicon Sequence Variant (ASVs)	981:1012	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	7	21	theme	Amplicon	981:988	arg1	ASVs					1008:1011	ASVs	1008:1011	ASVs	1008:1011	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	9	22	theme	bacterial	1469:1477	arg1	genera					1479:1484	5 bacterial genera	1467:1484	5 bacterial genera	1467:1484	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	2	23	theme	FODMAP	264:269	arg1	diet					347:350	FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet	264:350	FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet	264:350	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	10	24	from	useful	1643:1648	arg1	conclusion					1606:1615	conclusion	1606:1615	conclusion	1606:1615	In conclusion, bacterial biomarkers are useful to classify IBS individuals by responsiveness to LFD.					
33877245	1	25	theme	gastrointestinal	156:171	arg1	disorder					173:180	a functional gastrointestinal disorder	143:180	a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit	143:236	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	1	25	theme	gastrointestinal	156:171	arg1	Syndrome					125:132	Irritable Bowel Syndrome	109:132	Irritable Bowel Syndrome (IBS)	109:138	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	0	26	theme	microbiota	11:20	arg1	fingerprint					22:32	Intestinal microbiota fingerprint	0:32	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome	0:74	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	7	27	theme	abundance	959:967	arg1	analysis					969:976	Differential abundance analysis	946:976	Differential abundance analysis	946:976	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	4	28	dep	responders	512:521	arg1	patients					543:550	patients	543:550	IBS responders and non-responders' patients to LFD	508:557	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	3	29	theme	low-FODMAP	415:424	arg1	LFD					432:434	LFD	432:434	LFD	432:434	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	3	29	theme	low-FODMAP	415:424	arg1	diet					426:429	low-FODMAP diet	415:429	low-FODMAP diet (LFD)	415:435	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	0	30	theme	Intestinal	0:9	arg1	fingerprint					22:32	Intestinal microbiota fingerprint	0:32	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome	0:74	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	4	31	theme	IBS	508:510	arg1	responders					512:521	IBS responders	508:521	IBS responders	508:521	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	4	32	theme	16S	627:629	arg1	region					647:652	the 16S rRNA gene V3-V4 region	623:652	the 16S rRNA gene V3-V4 region	623:652	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	9	33	used	used	1388:1391	arg2	LDA					1378:1380	LDA	1378:1380	LDA	1378:1380	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	9	33	used	used	1388:1391	arg2	Analysis					1368:1375	Linear Discriminant Analysis	1348:1375	Linear Discriminant Analysis	1348:1375	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	5	34	theme	intervention	835:846	arg1	response					848:855	intervention response	835:855	intervention response	835:855	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	4	35	theme	microbial	589:597	arg1	composition					599:609	baseline fecal microbial composition	574:609	baseline fecal microbial composition	574:609	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	7	36	theme	Differential	946:957	arg1	analysis					969:976	Differential abundance analysis	946:976	Differential abundance analysis	946:976	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	5	37	dep	LFD	781:783	arg1	used					820:823	used	820:823	was used to assess intervention response	816:855	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	1	38	theme	abdominal	199:207	arg1	pain					209:212	abdominal pain	199:212	abdominal pain	199:212	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	9	39	theme	Discriminant	1355:1366	arg1	Analysis					1368:1375	Linear Discriminant Analysis	1348:1375	Linear Discriminant Analysis	1348:1375	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	9	39	theme	Discriminant	1355:1366	arg1	LDA					1378:1380	LDA	1378:1380	LDA	1378:1380	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	9	40	theme	baseline	1445:1452	arg1	abundance					1454:1462	baseline abundance	1445:1462	baseline abundance of 5 bacterial genera	1445:1484	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	4	41	theme	V3-V4	641:645	arg1	region					647:652	the 16S rRNA gene V3-V4 region	623:652	the 16S rRNA gene V3-V4 region	623:652	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	5	42	theme	four-week	771:779	arg1	Scale					802:806	Visual Analogue Scale	786:806	Visual Analogue Scale	786:806	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	5	42	theme	four-week	771:779	arg1	LFD					781:783	a four-week LFD	769:783	a four-week LFD	769:783	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	4	43	dep	composition	599:609	arg1	sequencing					612:621	sequencing	612:621	sequencing	612:621	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	5	44	with	participants	666:677	arg1	IBS					684:686	IBS	684:686	IBS	684:686	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	0	45	theme	bowel	61:65	arg1	syndrome					67:74	irritable bowel syndrome	51:74	irritable bowel syndrome	51:74	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	8	46	theme	abundant	1217:1224	arg1	ASVs					1226:1229	differentially abundant ASVs	1202:1229	differentially abundant ASVs	1202:1229	After LFD, differentially abundant ASVs were only identified in R, belonging to Veilonella, Butyrivibrio, and 5 ASVs belonging to Ruminiclostridium 6 genus.					
33877245	3	47	theme	%	464:464	arg1	%					468:468	50%-80%	462:468	50%-80% of IBS patients	462:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	3	47	theme	%	464:464	arg1	patients					477:484	IBS patients	473:484	IBS patients	473:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	0	48	theme	irritable	51:59	arg1	syndrome					67:74	irritable bowel syndrome	51:74	irritable bowel syndrome	51:74	Intestinal microbiota fingerprint in subjects with irritable bowel syndrome responders to a low FODMAP diet.					
33877245	7	49	dep	Veillonella	1055:1065	arg1	genus					1067:1071	Veillonella genus	1055:1071	Veillonella genus	1055:1071	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	10	50	theme	bacterial	1618:1626	arg1	biomarkers					1628:1637	bacterial biomarkers	1618:1637	bacterial biomarkers	1618:1637	In conclusion, bacterial biomarkers are useful to classify IBS individuals by responsiveness to LFD.					
33877245	7	51	theme	Ruminococcus	1146:1157	arg1	genus					1161:1165	Bifidobacterium and Ruminococcus 1 genus	1126:1165	genus	1161:1165	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	7	51	theme	Ruminococcus	1146:1157	arg1	Prevotella					1038:1047	Prevotella	1038:1047	Prevotella	1038:1047	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	1	52	theme	Bowel	119:123	arg1	Syndrome					125:132	Irritable Bowel Syndrome	109:132	Irritable Bowel Syndrome (IBS)	109:138	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	1	52	theme	Bowel	119:123	arg1	disorder					173:180	a functional gastrointestinal disorder	143:180	a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit	143:236	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	1	52	theme	Bowel	119:123	arg1	IBS					135:137	IBS	135:137	IBS	135:137	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	9	53	theme	LDA	1487:1489	arg1	model					1500:1504	LDA accuracy model	1487:1504	LDA accuracy model	1487:1504	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	8	54	theme	Ruminiclostridium	1321:1337	arg1	genus					1341:1345	Ruminiclostridium 6 genus	1321:1345	Ruminiclostridium 6 genus	1321:1345	After LFD, differentially abundant ASVs were only identified in R, belonging to Veilonella, Butyrivibrio, and 5 ASVs belonging to Ruminiclostridium 6 genus.					
33877245	5	55	used	used	820:823	arg2	IBS					812:814	IBS	812:814	IBS	812:814	Thirty-two participants with IBS were included, 29 women (90.62%) and three men (9.37%), and instructed to follow a four-week LFD, Visual Analogue Scale for IBS was used to assess intervention response.					
33877245	3	56	from	symptoms	450:457	arg1	%					468:468	50%-80%	462:468	50%-80% of IBS patients	462:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	3	56	from	symptoms	450:457	arg1	patients					477:484	IBS patients	473:484	IBS patients	473:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	7	57	theme	Bifidobacterium	1126:1140	arg1	genus					1161:1165	Bifidobacterium and Ruminococcus 1 genus	1126:1165	genus	1161:1165	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	7	57	theme	Bifidobacterium	1126:1140	arg1	Prevotella					1038:1047	Prevotella	1038:1047	Prevotella	1038:1047	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	1	58	theme	altered	218:224	arg1	habit					232:236	altered bowel habit	218:236	altered bowel habit	218:236	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	9	59	theme	100	1573:1575	arg1	%					1576:1576	%	1576:1576	%	1576:1576	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	6	60	dep	non-responders	921:934	arg1	%					942:942	31.25%	937:942	31.25%	937:942	Twenty-two participants were responders (68.75%), and ten were non-responders (31.25%).					
33877245	4	61	theme	gene	636:639	arg1	region					647:652	the 16S rRNA gene V3-V4 region	623:652	the 16S rRNA gene V3-V4 region	623:652	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	1	62	theme	bowel	226:230	arg1	habit					232:236	altered bowel habit	218:236	altered bowel habit	218:236	Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habit.					
33877245	9	63	theme	%	1576:1576	arg1	group					1596:1600	100% and non-responder group	1573:1600	100% and non-responder group	1573:1600	Linear Discriminant Analysis (LDA), was used to classify patients by responsiveness, considering baseline abundance of 5 bacterial genera, LDA accuracy model was 96.87%, correctly classifying 95.45% of in responder group and 100% and non-responder group.					
33877245	7	64	theme	non-responder	1170:1182	arg1	group					1184:1188	non-responder group	1170:1188	non-responder group	1170:1188	Differential abundance analysis of Amplicon Sequence Variant (ASVs), before LFD, identified Prevotella 9 and Veillonella genus in responder group, and Barnesiella, Paraprevotella, Bifidobacterium and Ruminococcus 1 genus in non-responder group.					
33877245	3	65	theme	IBS	473:475	arg1	patients					477:484	IBS patients	473:484	IBS patients	473:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	4	66	theme	baseline	574:581	arg1	composition					599:609	baseline fecal microbial composition	574:609	baseline fecal microbial composition	574:609	We aimed to identify IBS responders and non-responders' patients to LFD by determining baseline fecal microbial composition, sequencing the 16S rRNA gene V3-V4 region.					
33877245	2	67	theme	IBS	239:241	arg1	patients					243:250	IBS patients	239:250	IBS patients	239:250	IBS patients report that FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet induce or exacerbate their symptoms.					
33877245	3	68	theme	patients	477:484	arg1	%					468:468	50%-80%	462:468	50%-80% of IBS patients	462:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	3	68	theme	patients	477:484	arg1	patients					477:484	IBS patients	473:484	IBS patients	473:484	It has been reported that low-FODMAP diet (LFD) improves the symptoms in 50%-80% of IBS patients.					
33877245	10	69	theme	IBS	1662:1664	arg1	individuals					1666:1676	IBS individuals	1662:1676	IBS individuals	1662:1676	In conclusion, bacterial biomarkers are useful to classify IBS individuals by responsiveness to LFD.					
33607642	0	0	theme	mesenchymal	107:117	arg1	cells					124:128	bone marrow-derived mesenchymal stem cells	87:128	bone marrow-derived mesenchymal stem cells	87:128	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	9	1	theme	SA	1765:1766	arg1	solution					1768:1775	the SA solution	1761:1775	the SA solution	1761:1775	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	5	2	theme	Aker/SA	823:829	arg1	extract					805:811	the extract liquid	801:818	the extract liquid of Aker/SA	801:829	Specifically, we cultured macrophages (RAW 264.7 cell line) with the extract liquid of Aker/SA and assessed their phenotypic changes.					
33607642	2	3	theme	bone	372:375	arg1	regeneration					377:388	bone regeneration	372:388	bone regeneration	372:388	Previously, we developed an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration.					
33607642	4	4	theme	Aker/SA	594:600	arg1	hydrogel					602:609	Aker/SA hydrogel	594:609	Aker/SA hydrogel	594:609	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	0	5	theme	marrow-derived	92:105	arg1	cells					124:128	bone marrow-derived mesenchymal stem cells	87:128	bone marrow-derived mesenchymal stem cells	87:128	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	1	6	link	marrow-derived	232:245	arg1	cells					264:268	mesenchymal stem cells	247:268	bone marrow-derived mesenchymal stem cells (BMSCs)	227:276	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	1	6	link	marrow-derived	232:245	arg1	BMSCs					271:275	BMSCs	271:275	BMSCs	271:275	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	4	7	theme	bone	709:712	arg1	BMSCs					728:732	BMSCs	728:732	BMSCs	728:732	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	4	7	theme	bone	709:712	arg1	regeneration					714:725	bone regeneration	709:725	bone regeneration (BMSCs)	709:733	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	9	8	theme	BMSCs	1449:1453	arg1	migration					1436:1444	the migration	1432:1444	the migration of BMSCs	1432:1453	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	0	9	theme	cells	124:128	arg1	migration					74:82	migration	74:82	migration of bone marrow-derived mesenchymal stem cells	74:128	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	6	10	theme	×	893:893	arg1	105cells					895:902	2 × 105cells	891:902	2 × 105cells per 24 well	891:914	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	9	11	theme	SA	1607:1608	arg1	solution					1610:1617	the SA solution	1603:1617	the SA solution	1603:1617	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	0	12	theme	stem	119:122	arg1	cells					124:128	bone marrow-derived mesenchymal stem cells	87:128	bone marrow-derived mesenchymal stem cells	87:128	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	6	13	dep	macrophages	1009:1019	arg1	investigate					1024:1034	investigate	1024:1034	to investigate their effect on cell migration	1021:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	10	14	theme	mechanisms	1947:1956	arg1	one					1936:1938	one	1936:1938	one	1936:1938	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	10	14	theme	mechanisms	1947:1956	arg1	mechanisms					1947:1956	the mechanisms	1943:1956	the mechanisms through which the Aker/SA hydrogel accelerates new bone formation	1943:2022	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	1	15	used	used	165:168	arg2	Akermanite					131:140	Akermanite	131:140	Akermanite (Aker)	131:147	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	1	15	used	used	165:168	arg2	Aker					143:146	Aker	143:146	Aker	143:146	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	4	16	theme	hydrogel	602:609	arg1	implantable					579:589	implantable	579:589	implantable	579:589	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	1	17	theme	bone	174:177	arg1	regeneration					179:190	bone regeneration	174:190	bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs)	174:276	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	5	18	theme	cell	785:788	arg1	line					790:793	cell line	785:793	RAW 264.7 cell line	775:793	Specifically, we cultured macrophages (RAW 264.7 cell line) with the extract liquid of Aker/SA and assessed their phenotypic changes.					
33607642	6	19	from	effect	1042:1047	arg1	migration					1057:1065	cell migration	1052:1065	cell migration	1052:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	4	20	theme	inflammatory	668:679	arg1	hydrogel					658:665	Aker/SA hydrogel	650:665	Aker/SA hydrogel	650:665	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	4	20	theme	inflammatory	668:679	arg1	cells					681:685	inflammatory cells	668:685	inflammatory cells	668:685	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	7	21	dep	subcutaneously	1107:1120	arg1	ml-1					1136:1139	1 × 106cells ml-1	1123:1139	1 × 106cells ml-1	1123:1139	Finally, Aker/SA hydrogel was injected subcutaneously (1 × 106cells ml-1) in rat to verify its effectin vivo.					
33607642	3	22	from	effect	404:409	arg1	vivoresponse					448:459	thein vivoresponse	442:459	thein vivoresponse	442:459	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	3	22	from	effect	404:409	arg1	response					492:499	the inflammatory response	475:499	particularly the inflammatory response	462:499	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	7	23	theme	Aker/SA	1077:1083	arg1	hydrogel					1085:1092	Aker/SA hydrogel	1077:1092	Aker/SA hydrogel	1077:1092	Finally, Aker/SA hydrogel was injected subcutaneously (1 × 106cells ml-1) in rat to verify its effectin vivo.					
33607642	10	24	theme	implantation	1873:1884	arg1	site					1886:1889	the implantation site	1869:1889	the implantation site	1869:1889	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	5	25	theme	phenotypic	850:859	arg1	changes					861:867	their phenotypic changes	844:867	their phenotypic changes	844:867	Specifically, we cultured macrophages (RAW 264.7 cell line) with the extract liquid of Aker/SA and assessed their phenotypic changes.					
33607642	2	26	theme	alginate	330:337	arg1	hydrogel					349:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel	304:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration	304:388	Previously, we developed an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration.					
33607642	6	27	theme	Aker/SA	982:988	arg1	macrophages					1009:1019	Aker/SA hydrogel-activated macrophages	982:1019	Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration	982:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	3	28	theme	thein	442:446	arg1	vivoresponse					448:459	thein vivoresponse	442:459	thein vivoresponse	442:459	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	3	28	theme	thein	442:446	arg1	response					492:499	the inflammatory response	475:499	particularly the inflammatory response	462:499	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	0	29	theme	akermanite/alginate	25:43	arg1	hydrogel					55:62	akermanite/alginate composite hydrogel	25:62	akermanite/alginate composite hydrogel	25:62	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	6	30	theme	conditioned	946:956	arg1	that					974:977	that	974:977	that	974:977	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	6	30	theme	conditioned	946:956	arg1	media					958:962	different conditioned media	936:962	different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration	936:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	5	31	dep	RAW	775:777	arg1	line					790:793	cell line	785:793	RAW 264.7 cell line	775:793	Specifically, we cultured macrophages (RAW 264.7 cell line) with the extract liquid of Aker/SA and assessed their phenotypic changes.					
33607642	8	32	theme	Thein	1178:1182	arg1	vitroresults					1184:1195	Thein vitroresults	1178:1195	Thein vitroresults	1178:1195	Thein vitroresults indicated that Aker/SA hydrogel activated macrophages towards M2 phenotype and stimulated macrophages to express anti-inflammatory factors.					
33607642	2	33	theme	Aker/sodium	318:328	arg1	hydrogel					349:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel	304:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration	304:388	Previously, we developed an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration.					
33607642	3	34	theme	inflammatory	479:490	arg1	vivoresponse					448:459	thein vivoresponse	442:459	thein vivoresponse	442:459	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	3	34	theme	inflammatory	479:490	arg1	response					492:499	the inflammatory response	475:499	particularly the inflammatory response	462:499	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	6	35	theme	different	936:944	arg1	that					974:977	that	974:977	that	974:977	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	6	35	theme	different	936:944	arg1	media					958:962	different conditioned media	936:962	different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration	936:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	2	36	theme	injectable	307:316	arg1	hydrogel					349:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel	304:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration	304:388	Previously, we developed an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration.					
33607642	9	37	theme	M2	1560:1561	arg1	macrophages					1563:1573	more M2 macrophages	1555:1573	more M2 macrophages	1555:1573	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	8	38	theme	M2	1259:1260	arg1	phenotype					1262:1270	M2 phenotype	1259:1270	M2 phenotype	1259:1270	Thein vitroresults indicated that Aker/SA hydrogel activated macrophages towards M2 phenotype and stimulated macrophages to express anti-inflammatory factors.					
33607642	3	39	theme	injectable	419:428	arg1	hydrogel					430:437	this injectable hydrogel	414:437	this injectable hydrogel	414:437	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	5	40	dep	macrophages	762:772	arg1	RAW					775:777	RAW 264.7	775:783	RAW 264.7 cell line	775:793	Specifically, we cultured macrophages (RAW 264.7 cell line) with the extract liquid of Aker/SA and assessed their phenotypic changes.					
33607642	10	41	theme	Aker/SA	1813:1819	arg1	hydrogel					1821:1828	the Aker/SA hydrogel	1809:1828	the Aker/SA hydrogel	1809:1828	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	9	42	theme	thein	1480:1484	arg1	vitroresults					1486:1497	thein vitroresults	1480:1497	thein vitroresults	1480:1497	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	9	43	theme	more	1555:1558	arg1	macrophages					1563:1573	more M2 macrophages	1555:1573	more M2 macrophages	1555:1573	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	0	44	theme	composite	45:53	arg1	hydrogel					55:62	akermanite/alginate composite hydrogel	25:62	akermanite/alginate composite hydrogel	25:62	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	3	45	theme	hydrogel	430:437	arg1	effect					404:409	the effect	400:409	the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response,	400:500	However, the effect of this injectable hydrogel on thein vivoresponse, particularly the inflammatory response, has not been fully understood.					
33607642	5	46	theme	liquid	813:818	arg1	extract					805:811	the extract liquid	801:818	the extract liquid of Aker/SA	801:829	Specifically, we cultured macrophages (RAW 264.7 cell line) with the extract liquid of Aker/SA and assessed their phenotypic changes.					
33607642	1	47	theme	marrow-derived	232:245	arg1	cells					264:268	mesenchymal stem cells	247:268	bone marrow-derived mesenchymal stem cells (BMSCs)	227:276	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	1	47	theme	marrow-derived	232:245	arg1	BMSCs					271:275	BMSCs	271:275	BMSCs	271:275	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	7	48	theme	×	1125:1125	arg1	ml-1					1136:1139	1 × 106cells ml-1	1123:1139	1 × 106cells ml-1	1123:1139	Finally, Aker/SA hydrogel was injected subcutaneously (1 × 106cells ml-1) in rat to verify its effectin vivo.					
33607642	10	49	theme	Aker/SA	1976:1982	arg1	hydrogel					1984:1991	the Aker/SA hydrogel	1972:1991	the Aker/SA hydrogel	1972:1991	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	10	50	theme	immune	1847:1852	arg1	environment					1854:1864	the immune environment	1843:1864	the immune environment	1843:1864	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	0	51	link	marrow-derived	92:105	arg1	cells					124:128	bone marrow-derived mesenchymal stem cells	87:128	bone marrow-derived mesenchymal stem cells	87:128	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	9	52	theme	Aker/SA	1509:1515	arg1	hydrogel					1517:1524	the Aker/SA hydrogel	1505:1524	the Aker/SA hydrogel	1505:1524	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	9	53	theme	Aker-activated	1388:1401	arg1	macrophages					1403:1413	Aker-activated macrophages	1388:1413	Aker-activated macrophages	1388:1413	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	9	54	theme	more	1706:1709	arg1	BMSCs					1711:1715	more BMSCs	1706:1715	more BMSCs	1706:1715	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	9	55	theme	subcutaneous	1682:1693	arg1	injection					1695:1703	subcutaneous injection	1682:1703	subcutaneous injection	1682:1703	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	9	56	theme	Aker/SA	1739:1745	arg1	hydrogel					1747:1754	the Aker/SA hydrogel	1735:1754	the Aker/SA hydrogel than the SA solution	1735:1775	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	9	57	theme	conditioned	1354:1364	arg1	medium					1366:1371	the conditioned medium	1350:1371	the conditioned medium collected from Aker-activated macrophages	1350:1413	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	8	58	theme	anti-inflammatory	1310:1326	arg1	factors					1328:1334	anti-inflammatory factors	1310:1334	anti-inflammatory factors	1310:1334	Thein vitroresults indicated that Aker/SA hydrogel activated macrophages towards M2 phenotype and stimulated macrophages to express anti-inflammatory factors.					
33607642	10	59	theme	bone	2009:2012	arg1	formation					2014:2022	new bone formation	2005:2022	new bone formation	2005:2022	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	1	60	theme	mesenchymal	247:257	arg1	cells					264:268	mesenchymal stem cells	247:268	bone marrow-derived mesenchymal stem cells (BMSCs)	227:276	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	1	60	theme	mesenchymal	247:257	arg1	BMSCs					271:275	BMSCs	271:275	BMSCs	271:275	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	9	61	with	Consistent	1464:1473	arg1	vitroresults					1486:1497	thein vitroresults	1480:1497	thein vitroresults	1480:1497	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	6	62	theme	cell	1052:1055	arg1	migration					1057:1065	cell migration	1052:1065	cell migration	1052:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	2	63	theme	Aker/SA	340:346	arg1	hydrogel					349:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel	304:356	an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration	304:388	Previously, we developed an injectable Aker/sodium alginate (Aker/SA) hydrogel to facilitate bone regeneration.					
33607642	8	64	theme	Aker/SA	1212:1218	arg1	hydrogel					1220:1227	Aker/SA hydrogel	1212:1227	Aker/SA hydrogel	1212:1227	Thein vitroresults indicated that Aker/SA hydrogel activated macrophages towards M2 phenotype and stimulated macrophages to express anti-inflammatory factors.					
33607642	1	65	theme	stem	259:262	arg1	cells					264:268	mesenchymal stem cells	247:268	bone marrow-derived mesenchymal stem cells (BMSCs)	227:276	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	1	65	theme	stem	259:262	arg1	BMSCs					271:275	BMSCs	271:275	BMSCs	271:275	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	0	66	theme	bone	87:90	arg1	cells					124:128	bone marrow-derived mesenchymal stem cells	87:128	bone marrow-derived mesenchymal stem cells	87:128	Macrophages activated by akermanite/alginate composite hydrogel stimulate migration of bone marrow-derived mesenchymal stem cells.					
33607642	9	67	dep	d.	1640:1641	arg1	recruited					1722:1730	recruited	1722:1730	were recruited by the Aker/SA hydrogel than the SA solution	1717:1775	In addition, the conditioned medium collected from Aker-activated macrophages could accelerate the migration of BMSCs in 24 h. Consistent with thein vitroresults, when the Aker/SA hydrogel was injected subcutaneously, more M2 macrophages could be observed than when the SA solution was injected after 7 d. Besides, when BMSCs were delivered via subcutaneous injection, more BMSCs were recruited by the Aker/SA hydrogel than the SA solution.					
33607642	4	68	theme	Aker/SA	650:656	arg1	hydrogel					658:665	Aker/SA hydrogel	650:665	Aker/SA hydrogel	650:665	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	4	68	theme	Aker/SA	650:656	arg1	cells					681:685	inflammatory cells	668:685	inflammatory cells	668:685	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	4	68	theme	Aker/SA	650:656	arg1	cells					691:695	cells	691:695	cells	691:695	Here, to elucidate the response following the implantable of Aker/SA hydrogel, we investigated the interaction among Aker/SA hydrogel, inflammatory cells and cells involved in bone regeneration (BMSCs).					
33607642	1	69	theme	cells	264:268	arg1	osteogenesis					211:222	osteogenesis	211:222	osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs)	211:276	Akermanite (Aker) has been widely used for bone regeneration through regulating osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs).					
33607642	7	70	theme	106cells	1127:1134	arg1	ml-1					1136:1139	1 × 106cells ml-1	1123:1139	1 × 106cells ml-1	1123:1139	Finally, Aker/SA hydrogel was injected subcutaneously (1 × 106cells ml-1) in rat to verify its effectin vivo.					
33607642	10	71	theme	new	2005:2007	arg1	formation					2014:2022	new bone formation	2005:2022	new bone formation	2005:2022	All these results suggest that the Aker/SA hydrogel can modulate the immune environment at the implantation site and subsequently recruit BMSCs, which can be one of the mechanisms through which the Aker/SA hydrogel accelerates new bone formation.					
33607642	6	72	dep	BMSCs	884:888	arg1	105cells					895:902	2 × 105cells	891:902	2 × 105cells per 24 well	891:914	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
33607642	6	73	theme	hydrogel-activated	990:1007	arg1	macrophages					1009:1019	Aker/SA hydrogel-activated macrophages	982:1019	Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration	982:1065	Subsequently, BMSCs (2 × 105cells per 24 well) were cultured with different conditioned media including that of Aker/SA hydrogel-activated macrophages to investigate their effect on cell migration.					
32514366	14	0	theme	antibody	2367:2374	arg1	production					2376:2385	human antibody production	2361:2385	human antibody production	2361:2385	CONCLUSION These results demonstrated an alternative low-cost platform for human antibody production by using A. oryzae, possibly offering a reasonable expenditure for patient's welfare.					
32514366	2	1	theme	recombinant	323:333	arg1	antibody					335:342	the recombinant antibody	319:342	the recombinant antibody	319:342	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	6	2	theme	immune	1205:1210	arg1	cells					1212:1216	immune cells	1205:1216	immune cells	1205:1216	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	10	3	theme	antibody	1686:1693	arg1	antibody					1686:1693	antibody	1686:1693	antibody	1686:1693	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	10	3	theme	antibody	1686:1693	arg1	amount					1676:1681	the highest amount	1664:1681	the highest amount of antibody	1664:1693	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	13	4	theme	recombinant	2110:2120	arg1	adalimumab					2122:2131	the recombinant adalimumab	2106:2131	the recombinant adalimumab	2106:2131	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	6	5	theme	cell-mediated	1130:1142	arg1	ADCC					1158:1161	ADCC	1158:1161	ADCC	1158:1161	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	6	5	theme	cell-mediated	1130:1142	arg1	cytotoxicity					1144:1155	antibody-dependent cell-mediated cytotoxicity	1111:1155	antibody-dependent cell-mediated cytotoxicity (ADCC)	1111:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	14	6	theme	low-cost	2339:2346	arg1	platform					2348:2355	an alternative low-cost platform	2324:2355	an alternative low-cost platform for human antibody production	2324:2385	CONCLUSION These results demonstrated an alternative low-cost platform for human antibody production by using A. oryzae, possibly offering a reasonable expenditure for patient's welfare.					
32514366	2	7	theme	Chinese	268:274	arg1	ovaries					284:290	Chinese hamster ovaries	268:290	Chinese hamster ovaries (CHO)	268:296	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	7	theme	Chinese	268:274	arg1	CHO					293:295	CHO	293:295	CHO	293:295	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	5	8	theme	anti-TNFα	801:809	arg1	antibodies					864:873	the world's best-selling antibodies	839:873	the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis	839:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	8	theme	anti-TNFα	801:809	arg1	one					832:834	one	832:834	one	832:834	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	8	theme	anti-TNFα	801:809	arg1	adalimumab					820:829	the human anti-TNFα antibody adalimumab	791:829	the human anti-TNFα antibody adalimumab	791:829	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	12	9	theme	TNFα	1921:1924	arg1	comparable					1995:2004	comparable	1995:2004	comparable	1995:2004	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	12	9	theme	TNFα	1921:1924	arg1	activities					1939:1948	The antigen-binding and TNFα neutralizing activities	1897:1948	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae	1897:1988	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	9	10	theme	culture	1613:1619	arg1	supernatants					1621:1632	the culture supernatants	1609:1632	the culture supernatants	1609:1632	The full-length adalimumab consisting of two heavy and two light chains was successfully produced in the culture supernatants.					
32514366	4	11	theme	proteins	613:620	arg1	amount					603:608	a large amount	595:608	a large amount of proteins	595:620	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	11	theme	proteins	613:620	arg1	proteins					613:620	proteins	613:620	proteins	613:620	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	1	12	theme	therapeutic	183:193	arg1	applications					195:206	the current therapeutic applications	171:206	the current therapeutic applications	171:206	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	2	13	theme	cell	229:232	arg1	those					249:253	those	249:253	those	249:253	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	13	theme	cell	229:232	arg1	lines					234:238	Generally mammalian cell lines	209:238	Generally mammalian cell lines	209:238	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	7	14	theme	hyper-mannosylation	1313:1331	arg1	process					1333:1339	the hyper-mannosylation process	1309:1339	the hyper-mannosylation process	1309:1339	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	13	15	theme	immune	2259:2264	arg1	functions					2275:2283	immune effector functions	2259:2283	immune effector functions	2259:2283	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	13	16	theme	little	2229:2234	arg1	activity					2247:2254	only a little additional activity	2222:2254	only a little additional activity of immune effector functions	2222:2283	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	9	17	dep	heavy	1553:1557	arg1	chains					1573:1578	chains	1573:1578	chains	1573:1578	The full-length adalimumab consisting of two heavy and two light chains was successfully produced in the culture supernatants.					
32514366	11	18	theme	Two-step	1759:1766	arg1	purifications					1768:1780	Two-step purifications	1759:1780	Two-step purifications by Protein A and size-exclusion chromatography	1759:1827	Two-step purifications by Protein A and size-exclusion chromatography were applied to obtain the high purity sample for further analysis.					
32514366	13	19	theme	functions	2275:2283	arg1	activity					2247:2254	only a little additional activity	2222:2254	only a little additional activity of immune effector functions	2222:2283	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	12	20	theme	antigen-binding	1901:1915	arg1	comparable					1995:2004	comparable	1995:2004	comparable	1995:2004	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	12	20	theme	antigen-binding	1901:1915	arg1	activities					1939:1948	The antigen-binding and TNFα neutralizing activities	1897:1948	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae	1897:1988	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	7	21	theme	key	1294:1296	arg1	enzyme					1298:1303	a key enzyme	1292:1303	a key enzyme for the hyper-mannosylation process	1292:1339	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	12	22	theme	commercial	2022:2031	arg1	Humira®					2041:2047	a commercial product Humira®	2020:2047	a commercial product Humira®	2020:2047	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	5	23	theme	full	992:995	arg1	length					997:1002	the full length	988:1002	the full length of mAbs by A. oryzae	988:1023	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	10	24	theme	deletion	1730:1737	arg1	39.7 mg/L					1747:1755	39.7 mg/L	1747:1755	39.7 mg/L	1747:1755	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	10	24	theme	deletion	1730:1737	arg1	strain					1739:1744	the ten-protease deletion strain	1713:1744	the ten-protease deletion strain (39.7 mg/L)	1713:1756	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	7	25	dep	investigate	1353:1363	arg1	fungi					1344:1348	fungi	1344:1348	fungi	1344:1348	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	5	26	dep	chosen	970:975	arg1	Pursuing					768:775	Pursuing	768:775	Pursuing this strategy	768:789	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	13	27	theme	apparent	2053:2060	arg1	binding					2062:2068	No apparent binding	2050:2068	No apparent binding with the FcγRIIIa	2050:2086	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	5	28	theme	antibodies	864:873	arg1	adalimumab					820:829	the human anti-TNFα antibody adalimumab	791:829	the human anti-TNFα antibody adalimumab	791:829	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	28	theme	antibodies	864:873	arg1	one					832:834	one	832:834	one	832:834	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	28	theme	antibodies	864:873	arg1	antibodies					864:873	the world's best-selling antibodies	839:873	the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis	839:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	13	29	theme	additional	2236:2245	arg1	activity					2247:2254	only a little additional activity	2222:2254	only a little additional activity of immune effector functions	2222:2283	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	8	30	theme	RESULTS	1412:1418	arg1	Adalimumab					1420:1429	RESULTS Adalimumab	1412:1429	RESULTS Adalimumab	1412:1429	RESULTS Adalimumab was expressed in A. oryzae by the fusion protein system with α-amylase AmyB.					
32514366	7	31	theme	Aooch1	1271:1276	arg1	gene					1278:1281	the Aooch1 gene	1267:1281	the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process	1267:1339	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	6	32	gly	N-glycosylation	1037:1051	arg1	antibody					1060:1067	the antibody	1056:1067	the antibody	1056:1067	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	6	32	gly	N-glycosylation	1037:1051	arg1	effector					1084:1091	immune effector functions	1077:1101	immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC)	1077:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	3	33	dep	are	360:362	arg1	concerns					370:377	concerns	370:377	are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells	360:465	However, there are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells.					
32514366	5	34	theme	inflammatory	912:923	arg1	diseases					925:932	immune-mediated inflammatory diseases	896:932	immune-mediated inflammatory diseases including rheumatoid arthritis	896:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	34	theme	inflammatory	912:923	arg1	arthritis					955:963	rheumatoid arthritis	944:963	rheumatoid arthritis	944:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	0	35	theme	Functional	0:9	arg1	production					11:20	Functional production	0:20	Functional production of human antibody by the filamentous fungus Aspergillus oryzae	0:83	Functional production of human antibody by the filamentous fungus Aspergillus oryzae.					
32514366	4	36	dep	organism	559:566	arg1	Regarded					541:548	Regarded	541:548	Regarded As Safe	541:556	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	1	37	theme	Monoclonal	97:106	arg1	mAbs					120:123	mAbs	120:123	mAbs	120:123	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	1	37	theme	Monoclonal	97:106	arg1	antibodies					108:117	BACKGROUND Monoclonal antibodies	86:117	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals	86:146	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	8	38	theme	protein	1472:1478	arg1	system					1480:1485	the fusion protein system	1461:1485	the fusion protein system with α-amylase AmyB	1461:1505	RESULTS Adalimumab was expressed in A. oryzae by the fusion protein system with α-amylase AmyB.					
32514366	7	39	used	used	1246:1249	arg2	system					1235:1240	The CRISPR/Cas9 system	1219:1240	The CRISPR/Cas9 system	1219:1240	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	3	40	theme	contamination	426:438	arg1	cost					394:397	the high cost	385:397	the high cost	385:397	However, there are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells.					
32514366	3	40	theme	contamination	426:438	arg1	risk					407:410	the risk	403:410	the risk of pathogenic contamination	403:438	However, there are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells.					
32514366	13	41	theme	deletion	2190:2197	arg1	strain					2199:2204	the Aooch1 deletion strain	2179:2204	the Aooch1 deletion strain	2179:2204	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	9	42	theme	full-length	1512:1522	arg1	adalimumab					1524:1533	The full-length adalimumab	1508:1533	The full-length adalimumab consisting of two heavy and two light chains	1508:1578	The full-length adalimumab consisting of two heavy and two light chains was successfully produced in the culture supernatants.					
32514366	4	43	theme	protein	748:754	arg1	production					756:765	heterologous protein production	735:765	heterologous protein production	735:765	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	11	44	theme	purity	1861:1866	arg1	sample					1868:1873	the high purity sample	1852:1873	the high purity sample for further analysis	1852:1894	Two-step purifications by Protein A and size-exclusion chromatography were applied to obtain the high purity sample for further analysis.					
32514366	0	45	theme	antibody	31:38	arg1	production					11:20	Functional production	0:20	Functional production of human antibody by the filamentous fungus Aspergillus oryzae	0:83	Functional production of human antibody by the filamentous fungus Aspergillus oryzae.					
32514366	4	46	theme	filamentous	490:500	arg1	oryzae					480:485	Aspergillus oryzae	468:485	Aspergillus oryzae	468:485	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	46	theme	filamentous	490:500	arg1	fungus					502:507	a filamentous fungus	488:507	a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism	488:566	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	10	47	theme	producing	1645:1653	arg1	strains					1655:1661	the producing strains	1641:1661	the producing strains	1641:1661	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	12	48	dep	A.	1980:1981	arg1	oryzae					1983:1988	A. oryzae	1980:1988	A. oryzae	1980:1988	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	4	49	theme	microbial	715:723	arg1	hosts					725:729	the cost-effective microbial hosts	696:729	the cost-effective microbial hosts for heterologous protein production	696:765	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	10	50	theme	highest	1668:1674	arg1	antibody					1686:1693	antibody	1686:1693	antibody	1686:1693	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	10	50	theme	highest	1668:1674	arg1	amount					1676:1681	the highest amount	1664:1681	the highest amount of antibody	1664:1693	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	14	51	dep	CONCLUSION	2286:2295	arg1	demonstrated					2311:2322	demonstrated	2311:2322	demonstrated	2311:2322	CONCLUSION These results demonstrated an alternative low-cost platform for human antibody production by using A. oryzae, possibly offering a reasonable expenditure for patient's welfare.					
32514366	11	52	theme	size-exclusion	1799:1812	arg1	chromatography					1814:1827	size-exclusion chromatography	1799:1827	size-exclusion chromatography	1799:1827	Two-step purifications by Protein A and size-exclusion chromatography were applied to obtain the high purity sample for further analysis.					
32514366	4	53	used	used	681:684	arg2	fungus					665:670	the fungus	661:670	the fungus	661:670	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	53	used	used	681:684	arg2	one					689:691	one	689:691	one	689:691	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	53	used	used	681:684	arg2	hosts					725:729	the cost-effective microbial hosts	696:729	the cost-effective microbial hosts for heterologous protein production	696:765	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	8	54	dep	A.	1448:1449	arg1	oryzae					1451:1456	A. oryzae	1448:1456	A. oryzae	1448:1456	RESULTS Adalimumab was expressed in A. oryzae by the fusion protein system with α-amylase AmyB.					
32514366	14	55	theme	human	2361:2365	arg1	production					2376:2385	human antibody production	2361:2385	human antibody production	2361:2385	CONCLUSION These results demonstrated an alternative low-cost platform for human antibody production by using A. oryzae, possibly offering a reasonable expenditure for patient's welfare.					
32514366	7	56	theme	antibody	1388:1395	arg1	ability					1377:1383	the binding ability	1365:1383	the binding ability of antibody with FcγRIIIa	1365:1409	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	7	57	theme	binding	1369:1375	arg1	ability					1377:1383	the binding ability	1365:1383	the binding ability of antibody with FcγRIIIa	1365:1409	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	2	58	theme	hamster	276:282	arg1	ovaries					284:290	Chinese hamster ovaries	268:290	Chinese hamster ovaries (CHO)	268:296	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	58	theme	hamster	276:282	arg1	CHO					293:295	CHO	293:295	CHO	293:295	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	5	59	theme	antibody	811:818	arg1	antibodies					864:873	the world's best-selling antibodies	839:873	the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis	839:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	59	theme	antibody	811:818	arg1	one					832:834	one	832:834	one	832:834	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	59	theme	antibody	811:818	arg1	adalimumab					820:829	the human anti-TNFα antibody adalimumab	791:829	the human anti-TNFα antibody adalimumab	791:829	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	60	theme	human	795:799	arg1	antibodies					864:873	the world's best-selling antibodies	839:873	the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis	839:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	60	theme	human	795:799	arg1	one					832:834	one	832:834	one	832:834	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	60	theme	human	795:799	arg1	adalimumab					820:829	the human anti-TNFα antibody adalimumab	791:829	the human anti-TNFα antibody adalimumab	791:829	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	12	61	theme	adalimumab	1957:1966	arg1	comparable					1995:2004	comparable	1995:2004	comparable	1995:2004	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	12	61	theme	adalimumab	1957:1966	arg1	activities					1939:1948	The antigen-binding and TNFα neutralizing activities	1897:1948	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae	1897:1988	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	14	62	theme	reasonable	2427:2436	arg1	expenditure					2438:2448	a reasonable expenditure	2425:2448	a reasonable expenditure for patient's welfare	2425:2470	CONCLUSION These results demonstrated an alternative low-cost platform for human antibody production by using A. oryzae, possibly offering a reasonable expenditure for patient's welfare.					
32514366	6	63	theme	antibody-dependent	1111:1128	arg1	ADCC					1158:1161	ADCC	1158:1161	ADCC	1158:1161	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	6	63	theme	antibody-dependent	1111:1128	arg1	cytotoxicity					1144:1155	antibody-dependent cell-mediated cytotoxicity	1111:1155	antibody-dependent cell-mediated cytotoxicity (ADCC)	1111:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	4	64	theme	large	597:601	arg1	amount					603:608	a large amount	595:608	a large amount of proteins	595:620	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	64	theme	large	597:601	arg1	proteins					613:620	proteins	613:620	proteins	613:620	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	14	65	theme	alternative	2327:2337	arg1	platform					2348:2355	an alternative low-cost platform	2324:2355	an alternative low-cost platform for human antibody production	2324:2385	CONCLUSION These results demonstrated an alternative low-cost platform for human antibody production by using A. oryzae, possibly offering a reasonable expenditure for patient's welfare.					
32514366	10	66	theme	ten-protease	1717:1728	arg1	39.7 mg/L					1747:1755	39.7 mg/L	1747:1755	39.7 mg/L	1747:1755	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	10	66	theme	ten-protease	1717:1728	arg1	strain					1739:1744	the ten-protease deletion strain	1713:1744	the ten-protease deletion strain (39.7 mg/L)	1713:1756	Among the producing strains, the highest amount of antibody was obtained from the ten-protease deletion strain (39.7 mg/L).					
32514366	1	67	theme	current	175:181	arg1	applications					195:206	the current therapeutic applications	171:206	the current therapeutic applications	171:206	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	12	68	with	comparable	1995:2004	arg1	those					2011:2015	those	2011:2015	those	2011:2015	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	12	69	theme	neutralizing	1926:1937	arg1	comparable					1995:2004	comparable	1995:2004	comparable	1995:2004	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	12	69	theme	neutralizing	1926:1937	arg1	activities					1939:1948	The antigen-binding and TNFα neutralizing activities	1897:1948	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae	1897:1988	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	6	70	theme	effector	1084:1091	arg1	cytotoxicity					1144:1155	antibody-dependent cell-mediated cytotoxicity	1111:1155	antibody-dependent cell-mediated cytotoxicity (ADCC)	1111:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	6	70	theme	effector	1084:1091	arg1	functions					1093:1101	immune effector functions	1077:1101	immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC)	1077:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	13	71	theme	effector	2266:2273	arg1	functions					2275:2283	immune effector functions	2259:2283	immune effector functions	2259:2283	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	6	72	theme	Fc	1183:1184	arg1	FcγR					1196:1199	FcγR	1196:1199	FcγR	1196:1199	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	6	72	theme	Fc	1183:1184	arg1	receptor					1186:1193	Fc receptor	1183:1193	the Fc receptor (FcγR) on immune cells	1179:1216	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	4	73	dep	Regarded	541:548	arg1	Generally					531:539	Generally	531:539	Generally	531:539	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	8	74	with	system	1480:1485	arg1	AmyB					1502:1505	α-amylase AmyB	1492:1505	α-amylase AmyB	1492:1505	RESULTS Adalimumab was expressed in A. oryzae by the fusion protein system with α-amylase AmyB.					
32514366	4	75	contain	has	569:571	arg1	oryzae					480:485	Aspergillus oryzae	468:485	Aspergillus oryzae	468:485	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	75	contain	has	569:571	arg1	fungus					502:507	a filamentous fungus	488:507	a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism	488:566	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	75	contain	has	569:571	arg2	ability					576:582	an ability to secrete a large amount of proteins into the culture supernatant	573:649	an ability to secrete a large amount of proteins into the culture supernatant	573:649	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	5	76	theme	best-selling	851:862	arg1	antibodies					864:873	the world's best-selling antibodies	839:873	the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis	839:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	2	77	attach	derived	255:261	arg1	ovaries					284:290	Chinese hamster ovaries	268:290	Chinese hamster ovaries (CHO)	268:296	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	77	attach	derived	255:261	arg2	those					249:253	those	249:253	those	249:253	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	77	attach	derived	255:261	arg1	CHO					293:295	CHO	293:295	CHO	293:295	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	12	78	theme	product	2033:2039	arg1	Humira®					2041:2047	a commercial product Humira®	2020:2047	a commercial product Humira®	2020:2047	The antigen-binding and TNFα neutralizing activities of the adalimumab produced by A. oryzae were comparable with those of a commercial product Humira®.					
32514366	6	79	from	receptor	1186:1193	arg1	cells					1212:1216	immune cells	1205:1216	immune cells	1205:1216	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	0	80	theme	Aspergillus	66:76	arg1	oryzae					78:83	the filamentous fungus Aspergillus oryzae	43:83	the filamentous fungus Aspergillus oryzae	43:83	Functional production of human antibody by the filamentous fungus Aspergillus oryzae.					
32514366	5	81	theme	mAbs	1007:1010	arg1	length					997:1002	the full length	988:1002	the full length of mAbs by A. oryzae	988:1023	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	1	82	theme	pivotal	155:161	arg1	role					163:166	a pivotal role	153:166	a pivotal role	153:166	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	4	83	theme	culture	631:637	arg1	supernatant					639:649	the culture supernatant	627:649	the culture supernatant	627:649	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	5	84	theme	immune-mediated	896:910	arg1	diseases					925:932	immune-mediated inflammatory diseases	896:932	immune-mediated inflammatory diseases including rheumatoid arthritis	896:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	5	84	theme	immune-mediated	896:910	arg1	arthritis					955:963	rheumatoid arthritis	944:963	rheumatoid arthritis	944:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	3	85	theme	mammalian	451:459	arg1	cells					461:465	mammalian cells	451:465	mammalian cells	451:465	However, there are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells.					
32514366	5	86	theme	diseases	925:932	arg1	treatment					883:891	the treatment	879:891	the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis	879:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	13	87	with	binding	2062:2068	arg1	FcγRIIIa					2079:2086	the FcγRIIIa	2075:2086	the FcγRIIIa	2075:2086	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	8	88	theme	fusion	1465:1470	arg1	system					1480:1485	the fusion protein system	1461:1485	the fusion protein system with α-amylase AmyB	1461:1505	RESULTS Adalimumab was expressed in A. oryzae by the fusion protein system with α-amylase AmyB.					
32514366	2	89	used	used	303:306	arg2	those					249:253	those	249:253	those	249:253	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	89	used	used	303:306	arg2	lines					234:238	Generally mammalian cell lines	209:238	Generally mammalian cell lines	209:238	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	11	90	theme	high	1856:1859	arg1	sample					1868:1873	the high purity sample	1852:1873	the high purity sample for further analysis	1852:1894	Two-step purifications by Protein A and size-exclusion chromatography were applied to obtain the high purity sample for further analysis.					
32514366	1	91	theme	BACKGROUND	86:95	arg1	mAbs					120:123	mAbs	120:123	mAbs	120:123	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	1	91	theme	BACKGROUND	86:95	arg1	antibodies					108:117	BACKGROUND Monoclonal antibodies	86:117	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals	86:146	BACKGROUND Monoclonal antibodies (mAbs) as biopharmaceuticals take a pivotal role in the current therapeutic applications.					
32514366	4	92	theme	GRAS	525:528	arg1	organism					559:566	a GRAS (Generally Regarded As Safe) organism	523:566	a GRAS (Generally Regarded As Safe) organism	523:566	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	0	93	theme	human	25:29	arg1	antibody					31:38	human antibody	25:38	human antibody	25:38	Functional production of human antibody by the filamentous fungus Aspergillus oryzae.					
32514366	13	94	theme	Aooch1	2183:2188	arg1	strain					2199:2204	the Aooch1 deletion strain	2179:2204	the Aooch1 deletion strain	2179:2204	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	8	95	theme	α-amylase	1492:1500	arg1	AmyB					1502:1505	α-amylase AmyB	1492:1505	α-amylase AmyB	1492:1505	RESULTS Adalimumab was expressed in A. oryzae by the fusion protein system with α-amylase AmyB.					
32514366	11	96	theme	further	1879:1885	arg1	analysis					1887:1894	further analysis	1879:1894	further analysis	1879:1894	Two-step purifications by Protein A and size-exclusion chromatography were applied to obtain the high purity sample for further analysis.					
32514366	0	97	dep	filamentous	47:57	arg1	fungus					59:64	fungus	59:64	fungus	59:64	Functional production of human antibody by the filamentous fungus Aspergillus oryzae.					
32514366	7	98	theme	CRISPR/Cas9	1223:1233	arg1	system					1235:1240	The CRISPR/Cas9 system	1219:1240	The CRISPR/Cas9 system	1219:1240	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	3	99	theme	high	389:392	arg1	cost					394:397	the high cost	385:397	the high cost	385:397	However, there are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells.					
32514366	4	100	theme	heterologous	735:746	arg1	production					756:765	heterologous protein production	735:765	heterologous protein production	735:765	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	3	101	theme	pathogenic	415:424	arg1	contamination					426:438	pathogenic contamination	415:438	pathogenic contamination	415:438	However, there are still concerns about the high cost and the risk of pathogenic contamination when using mammalian cells.					
32514366	0	102	theme	filamentous	47:57	arg1	oryzae					78:83	the filamentous fungus Aspergillus oryzae	43:83	the filamentous fungus Aspergillus oryzae	43:83	Functional production of human antibody by the filamentous fungus Aspergillus oryzae.					
32514366	13	103	theme	N-glycan	2154:2161	arg1	structure					2163:2171	the N-glycan structure	2150:2171	the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions	2150:2283	No apparent binding with the FcγRIIIa was detected with the recombinant adalimumab even by altering the N-glycan structure using the Aooch1 deletion strain, which suggests only a little additional activity of immune effector functions.					
32514366	6	104	theme	immune	1077:1082	arg1	cytotoxicity					1144:1155	antibody-dependent cell-mediated cytotoxicity	1111:1155	antibody-dependent cell-mediated cytotoxicity (ADCC)	1111:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	6	104	theme	immune	1077:1082	arg1	functions					1093:1101	immune effector functions	1077:1101	immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC)	1077:1162	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	2	105	theme	mammalian	219:227	arg1	those					249:253	those	249:253	those	249:253	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	2	105	theme	mammalian	219:227	arg1	lines					234:238	Generally mammalian cell lines	209:238	Generally mammalian cell lines	209:238	Generally mammalian cell lines, such as those derived from Chinese hamster ovaries (CHO), are used to produce the recombinant antibody.					
32514366	5	106	theme	rheumatoid	944:953	arg1	arthritis					955:963	rheumatoid arthritis	944:963	rheumatoid arthritis	944:963	Pursuing this strategy the human anti-TNFα antibody adalimumab, one of the world's best-selling antibodies for the treatment of immune-mediated inflammatory diseases including rheumatoid arthritis, was chosen to produce the full length of mAbs by A. oryzae.					
32514366	6	107	theme	antibody	1060:1067	arg1	N-glycosylation					1037:1051	N-glycosylation	1037:1051	N-glycosylation of the antibody	1037:1067	Generally, N-glycosylation of the antibody affects immune effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) via binding to the Fc receptor (FcγR) on immune cells.					
32514366	4	108	theme	cost-effective	700:713	arg1	hosts					725:729	the cost-effective microbial hosts	696:729	the cost-effective microbial hosts for heterologous protein production	696:765	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	11	109	theme	Protein	1785:1791	arg1	A					1793:1793	Protein A	1785:1793	Protein A	1785:1793	Two-step purifications by Protein A and size-exclusion chromatography were applied to obtain the high purity sample for further analysis.					
32514366	7	110	with	antibody	1388:1395	arg1	FcγRIIIa					1402:1409	FcγRIIIa	1402:1409	FcγRIIIa	1402:1409	The CRISPR/Cas9 system was used to first delete the Aooch1 gene encoding a key enzyme for the hyper-mannosylation process in fungi to investigate the binding ability of antibody with FcγRIIIa.					
32514366	4	111	theme	hosts	725:729	arg1	hosts					725:729	the cost-effective microbial hosts	696:729	the cost-effective microbial hosts for heterologous protein production	696:765	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	111	theme	hosts	725:729	arg1	one					689:691	one	689:691	one	689:691	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
32514366	4	111	theme	hosts	725:729	arg1	fungus					665:670	the fungus	661:670	the fungus	661:670	Aspergillus oryzae, a filamentous fungus recognized as a GRAS (Generally Regarded As Safe) organism, has an ability to secrete a large amount of proteins into the culture supernatant, and thus the fungus has been used as one of the cost-effective microbial hosts for heterologous protein production.					
34022704	5	0	theme	optimized	816:824	arg1	formulation					826:836	The optimized formulation	812:836	The optimized formulation	812:836	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	3	1	theme	lipid	544:548	arg1	SLNs					565:568	SLNs	565:568	SLNs	565:568	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	3	1	theme	lipid	544:548	arg1	nanoparticles					550:562	chitosan-coated solid lipid nanoparticles	522:562	chitosan-coated solid lipid nanoparticles (SLNs)	522:569	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	9	2	theme	rat	1674:1676	arg1	organs					1678:1683	different rat organs	1664:1683	different rat organs	1664:1683	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	8	3	located	observed	1607:1614	arg2	improvement					1394:1404	Substantial improvement	1382:1404	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain	1382:1601	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	8	3	located	observed	1607:1614	arg1	rats					1630:1633	AD-induced rats	1619:1633	AD-induced rats	1619:1633	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	6	4	theme	uniform	1160:1166	arg1	nanoparticles					1178:1190	uniform spherical nanoparticles	1160:1190	uniform spherical nanoparticles	1160:1190	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	10	5	theme	lipidic	1972:1978	arg1	nanoconstructs					1980:1993	the developed lipidic nanoconstructs	1958:1993	the developed lipidic nanoconstructs of FA	1958:1999	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	3	6	theme	QbD	577:579	arg1	paradigms					581:589	QbD paradigms	577:589	QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD)	577:663	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	8	7	from	improvement	1394:1404	arg1	ability					1429:1435	cognitive ability	1419:1435	cognitive ability	1419:1435	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	9	8	theme	perceptible	1695:1705	arg1	s					1714:1714	s	1714:1714	s	1714:1714	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	9	8	theme	perceptible	1695:1705	arg1	change					1707:1712	no perceptible change	1692:1712	no perceptible change(s) in tissue morphology	1692:1736	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	4	9	theme	FA	674:675	arg1	SLNs					666:669	SLNs	666:669	SLNs of FA	666:675	SLNs of FA were formulated employing Compritol as lipid and polysorbate 80 as surfactant and optimised using a 32 Central Composite Design (CCD).					
34022704	8	10	with	improvement	1394:1404	arg1	SLNs					1411:1414	SLNs	1411:1414	SLNs	1411:1414	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	10	11	theme	improved	1800:1807	arg1	efficacy					1817:1824	improved anti-AD efficacy	1800:1824	improved anti-AD efficacy	1800:1824	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	5	12	theme	ionic	874:878	arg1	gelation					880:887	ionic gelation	874:887	ionic gelation	874:887	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	9	13	from	change	1707:1712	arg1	morphology					1727:1736	tissue morphology	1720:1736	tissue morphology	1720:1736	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	5	14	theme	entrapment	925:934	arg1	efficiency					936:945	entrapment efficiency	925:945	entrapment efficiency of 51.2 %	925:955	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	1	15	theme	natural	189:195	arg1	acid					163:166	Ferulic acid	155:166	Ferulic acid (FA)	155:171	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	1	15	theme	natural	189:195	arg1	plant					197:201	a ubiquitous natural plant	176:201	a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders	176:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	3	16	theme	current	452:458	arg1	studies					460:466	The current studies	448:466	The current studies	448:466	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	0	17	theme	preclinical	133:143	arg1	evidence					145:152	A preclinical evidence	131:152	A preclinical evidence	131:152	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	10	18	theme	superior	1827:1834	arg1	mucoadhesion					1842:1853	superior nasal mucoadhesion	1827:1853	superior nasal mucoadhesion	1827:1853	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	1	19	theme	bioactive	203:211	arg1	acid					163:166	Ferulic acid	155:166	Ferulic acid (FA)	155:171	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	1	19	theme	bioactive	203:211	arg1	plant					197:201	a ubiquitous natural plant	176:201	a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders	176:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	9	20	theme	tissue	1720:1725	arg1	morphology					1727:1736	tissue morphology	1720:1736	tissue morphology	1720:1736	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	3	21	theme	FA	616:617	arg1	efficacy					604:611	improved efficacy	595:611	improved efficacy of FA in the management of Alzheimer's disease (AD)	595:663	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	7	22	theme	drug	1344:1347	arg1	release					1349:1355	in vitro drug release	1335:1355	in vitro drug release	1335:1355	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	6	23	theme	little	1197:1202	arg1	aggregation					1204:1214	little aggregation	1197:1214	little aggregation	1197:1214	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	5	24	theme	nm	921:922	arg1	efficiency					936:945	entrapment efficiency	925:945	entrapment efficiency of 51.2 %	925:955	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	24	theme	nm	921:922	arg1	potential					966:974	zeta potential	961:974	zeta potential of 12.4 mV	961:985	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	24	theme	nm	921:922	arg1	size					909:912	particle size	900:912	particle size of 185 nm	900:922	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	8	25	theme	latency	1469:1475	arg1	time					1477:1480	escape latency time	1462:1480	escape latency time during behavioural studies	1462:1507	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	7	26	theme	in	1335:1336	arg1	release					1349:1355	in vitro drug release	1335:1355	in vitro drug release	1335:1355	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	3	27	from	efficacy	604:611	arg1	management					626:635	the management	622:635	the management of Alzheimer's disease (AD)	622:663	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	0	28	theme	acid	77:80	arg1	nanoparticles					52:64	Systematically designed chitosan-coated solid lipid nanoparticles	0:64	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease	0:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	1	29	theme	Ferulic	155:161	arg1	plant					197:201	a ubiquitous natural plant	176:201	a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders	176:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	1	29	theme	Ferulic	155:161	arg1	FA					169:170	FA	169:170	FA	169:170	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	1	29	theme	Ferulic	155:161	arg1	acid					163:166	Ferulic acid	155:166	Ferulic acid (FA)	155:171	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	0	30	theme	effective	86:94	arg1	management					96:105	effective management	86:105	effective management of Alzheimer's disease	86:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	5	31	theme	particle	900:907	arg1	size					909:912	particle size	900:912	particle size of 185 nm	900:922	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	2	32	theme	therapeutic	283:293	arg1	efficacy					295:302	its therapeutic efficacy	279:302	its therapeutic efficacy	279:302	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	5	33	theme	%	955:955	arg1	efficiency					936:945	entrapment efficiency	925:945	entrapment efficiency of 51.2 %	925:955	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	33	theme	%	955:955	arg1	potential					966:974	zeta potential	961:974	zeta potential of 12.4 mV	961:985	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	33	theme	%	955:955	arg1	size					909:912	particle size	900:912	particle size of 185 nm	900:922	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	34	theme	zeta	961:964	arg1	potential					966:974	zeta potential	961:974	zeta potential of 12.4 mV	961:985	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	8	35	theme	significant	1524:1534	arg1	improvement					1536:1546	significant improvement	1524:1546	significant improvement in various biochemical parameters and body weight gain	1524:1601	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	7	36	theme	Notable	1217:1223	arg1	improvement					1225:1235	Notable improvement	1217:1235	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release,	1217:1356	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	7	37	from	improvement	1225:1235	arg1	mucoadhesion					1248:1259	ex vivo mucoadhesion	1240:1259	ex vivo mucoadhesion	1240:1259	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	7	37	from	improvement	1225:1235	arg1	studies					1276:1282	permeation studies	1265:1282	permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release,	1265:1356	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	7	38	dep	ex	1240:1241	arg1	vivo					1243:1246	vivo	1243:1246	vivo	1243:1246	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	6	39	theme	nanoparticles	1178:1190	arg1	existence					1147:1155	existence	1147:1155	existence of uniform spherical nanoparticles	1147:1190	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	0	40	theme	chitosan-coated	24:38	arg1	nanoparticles					52:64	Systematically designed chitosan-coated solid lipid nanoparticles	0:64	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease	0:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	1	41	with	bioactive	203:211	arg1	promise					230:236	distinctive promise	218:236	distinctive promise in neurodegenerative disorders	218:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	8	42	theme	biochemical	1559:1569	arg1	parameters					1571:1580	various biochemical parameters	1551:1580	various biochemical parameters	1551:1580	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	2	43	theme	first-pass	425:434	arg1	metabolism					436:445	extensive first-pass metabolism	415:445	extensive first-pass metabolism	415:445	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	0	44	theme	lipid	46:50	arg1	nanoparticles					52:64	Systematically designed chitosan-coated solid lipid nanoparticles	0:64	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease	0:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	7	45	theme	nasal	1295:1299	arg1	mucosa					1301:1306	goat nasal mucosa	1290:1306	goat nasal mucosa	1290:1306	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	10	46	theme	extended	1871:1878	arg1	release					1885:1891	extended drug release	1871:1891	extended drug release	1871:1891	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	47	theme	drug	1880:1883	arg1	release					1885:1891	extended drug release	1871:1891	extended drug release	1871:1891	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	8	48	theme	weight	1591:1596	arg1	gain					1598:1601	body weight gain	1586:1601	body weight gain	1586:1601	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	4	49	theme	Composite	788:796	arg1	Design					798:803	a 32 Central Composite Design	775:803	a 32 Central Composite Design (CCD)	775:809	SLNs of FA were formulated employing Compritol as lipid and polysorbate 80 as surfactant and optimised using a 32 Central Composite Design (CCD).					
34022704	4	49	theme	Composite	788:796	arg1	CCD					806:808	CCD	806:808	CCD	806:808	SLNs of FA were formulated employing Compritol as lipid and polysorbate 80 as surfactant and optimised using a 32 Central Composite Design (CCD).					
34022704	2	50	theme	poor	334:337	arg1	solubility					347:356	its poor aqueous solubility	330:356	its poor aqueous solubility	330:356	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	6	51	theme	significant	1086:1096	arg1	loss					1098:1101	PXRD construed significant loss	1071:1101	PXRD construed significant loss of drug crystallinity	1071:1123	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	10	52	theme	improved	1894:1901	arg1	compliance					1911:1920	improved patient compliance potential	1894:1930	improved patient compliance potential	1894:1930	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	8	53	theme	cognitive	1419:1427	arg1	ability					1429:1435	cognitive ability	1419:1435	cognitive ability	1419:1435	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	5	54	theme	mV	984:985	arg1	efficiency					936:945	entrapment efficiency	925:945	entrapment efficiency of 51.2 %	925:955	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	54	theme	mV	984:985	arg1	potential					966:974	zeta potential	961:974	zeta potential of 12.4 mV	961:985	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	5	54	theme	mV	984:985	arg1	size					909:912	particle size	900:912	particle size of 185 nm	900:922	The optimized formulation, surface-coated with chitosan using ionic gelation, exhibited particle size of 185 nm, entrapment efficiency of 51.2 % and zeta potential of 12.4 mV.					
34022704	7	55	from	extension	1322:1330	arg1	release					1349:1355	in vitro drug release	1335:1355	in vitro drug release	1335:1355	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	2	56	theme	inadequate	359:368	arg1	permeability					370:381	inadequate permeability	359:381	inadequate permeability across lipophilic barriers	359:408	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	1	57	theme	neurodegenerative	241:257	arg1	disorders					259:267	neurodegenerative disorders	241:267	neurodegenerative disorders	241:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	9	58	theme	different	1664:1672	arg1	organs					1678:1683	different rat organs	1664:1683	different rat organs	1664:1683	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	7	59	theme	ex	1240:1241	arg1	mucoadhesion					1248:1259	ex vivo mucoadhesion	1240:1259	ex vivo mucoadhesion	1240:1259	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	10	60	theme	anti-AD	1809:1815	arg1	efficacy					1817:1824	improved anti-AD efficacy	1800:1824	improved anti-AD efficacy	1800:1824	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	9	61	theme	organs	1678:1683	arg1	images					1654:1659	Histopathological images	1636:1659	Histopathological images of different rat organs	1636:1683	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	10	62	theme	developed	1962:1970	arg1	nanoconstructs					1980:1993	the developed lipidic nanoconstructs	1958:1993	the developed lipidic nanoconstructs of FA	1958:1999	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	3	63	theme	improved	595:602	arg1	efficacy					604:611	improved efficacy	595:611	improved efficacy of FA in the management of Alzheimer's disease (AD)	595:663	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	10	64	theme	FA	1998:1999	arg1	nanoconstructs					1980:1993	the developed lipidic nanoconstructs	1958:1993	the developed lipidic nanoconstructs of FA	1958:1999	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	7	65	dep	in	1335:1336	arg1	vitro					1338:1342	vitro	1338:1342	vitro	1338:1342	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	6	66	theme	crystallinity	1111:1123	arg1	loss					1098:1101	PXRD construed significant loss	1071:1101	PXRD construed significant loss of drug crystallinity	1071:1123	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	3	67	theme	solid	538:542	arg1	SLNs					565:568	SLNs	565:568	SLNs	565:568	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	3	67	theme	solid	538:542	arg1	nanoparticles					550:562	chitosan-coated solid lipid nanoparticles	522:562	chitosan-coated solid lipid nanoparticles (SLNs)	522:569	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	2	68	theme	aqueous	339:345	arg1	solubility					347:356	its poor aqueous solubility	330:356	its poor aqueous solubility	330:356	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	10	69	theme	nasal	1836:1840	arg1	mucoadhesion					1842:1853	superior nasal mucoadhesion	1827:1853	superior nasal mucoadhesion	1827:1853	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	1	70	from	promise	230:236	arg1	disorders					259:267	neurodegenerative disorders	241:267	neurodegenerative disorders	241:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	8	71	theme	escape	1462:1467	arg1	time					1477:1480	escape latency time	1462:1480	escape latency time during behavioural studies	1462:1507	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	10	72	theme	intranasal	2009:2018	arg1	route					2020:2024	intranasal route	2009:2024	intranasal route	2009:2024	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	6	73	theme	DSC	997:999	arg1	studies					1001:1007	FTIR and DSC studies	988:1007	FTIR and DSC studies	988:1007	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	8	74	from	reduction	1449:1457	arg1	time					1477:1480	escape latency time	1462:1480	escape latency time during behavioural studies	1462:1507	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	1	75	theme	ubiquitous	178:187	arg1	acid					163:166	Ferulic acid	155:166	Ferulic acid (FA)	155:171	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	1	75	theme	ubiquitous	178:187	arg1	plant					197:201	a ubiquitous natural plant	176:201	a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders	176:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	0	76	dep	evidence	145:152	arg1	nanoparticles					52:64	Systematically designed chitosan-coated solid lipid nanoparticles	0:64	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease	0:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	6	77	theme	FTIR	988:991	arg1	studies					1001:1007	FTIR and DSC studies	988:1007	FTIR and DSC studies	988:1007	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	8	78	theme	behavioural	1489:1499	arg1	studies					1501:1507	behavioural studies	1489:1507	behavioural studies	1489:1507	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	6	79	theme	spherical	1168:1176	arg1	nanoparticles					1178:1190	uniform spherical nanoparticles	1160:1190	uniform spherical nanoparticles	1160:1190	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	7	80	theme	permeation	1265:1274	arg1	studies					1276:1282	permeation studies	1265:1282	permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release,	1265:1356	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	6	81	theme	formulation	1047:1057	arg1	excipients					1059:1068	formulation excipients	1047:1068	formulation excipients	1047:1068	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	0	82	theme	designed	15:22	arg1	nanoparticles					52:64	Systematically designed chitosan-coated solid lipid nanoparticles	0:64	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease	0:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	release					1885:1891	extended drug release	1871:1891	extended drug release	1871:1891	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	safety					1933:1938	safety	1933:1938	safety	1933:1938	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	mucoadhesion					1842:1853	superior nasal mucoadhesion	1827:1853	superior nasal mucoadhesion	1827:1853	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	robustness					1944:1953	robustness	1944:1953	robustness	1944:1953	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	permeation					1859:1868	permeation	1859:1868	permeation	1859:1868	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	efficacy					1817:1824	improved anti-AD efficacy	1800:1824	improved anti-AD efficacy	1800:1824	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	10	83	theme	nanoconstructs	1980:1993	arg1	compliance					1911:1920	improved patient compliance potential	1894:1930	improved patient compliance potential	1894:1930	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	6	84	theme	FA	1039:1040	arg1	compatibility					1022:1034	the compatibility	1018:1034	the compatibility of FA	1018:1040	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	8	85	theme	various	1551:1557	arg1	parameters					1571:1580	various biochemical parameters	1551:1580	various biochemical parameters	1551:1580	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	2	86	theme	lipophilic	390:399	arg1	barriers					401:408	lipophilic barriers	390:408	lipophilic barriers	390:408	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	0	87	theme	solid	40:44	arg1	nanoparticles					52:64	Systematically designed chitosan-coated solid lipid nanoparticles	0:64	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease	0:128	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	10	88	theme	preclinical	1754:1764	arg1	findings					1766:1773	these preclinical findings	1748:1773	these preclinical findings	1748:1773	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	8	89	from	improvement	1536:1546	arg1	parameters					1571:1580	various biochemical parameters	1551:1580	various biochemical parameters	1551:1580	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	8	89	from	improvement	1536:1546	arg1	gain					1598:1601	body weight gain	1586:1601	body weight gain	1586:1601	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	7	90	theme	goat	1290:1293	arg1	mucosa					1301:1306	goat nasal mucosa	1290:1306	goat nasal mucosa	1290:1306	Notable improvement in ex vivo mucoadhesion and permeation studies using goat nasal mucosa, coupled with extension in in vitro drug release, was obtained with SLNs.					
34022704	8	91	theme	body	1586:1589	arg1	gain					1598:1601	body weight gain	1586:1601	body weight gain	1586:1601	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	3	92	theme	disease	652:658	arg1	management					626:635	the management	622:635	the management of Alzheimer's disease (AD)	622:663	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	2	93	theme	extensive	415:423	arg1	metabolism					436:445	extensive first-pass metabolism	415:445	extensive first-pass metabolism	415:445	However, its therapeutic efficacy gets compromised owing to its poor aqueous solubility, inadequate permeability across lipophilic barriers, and extensive first-pass metabolism.					
34022704	0	94	theme	ferulic	69:75	arg1	acid					77:80	ferulic acid	69:80	ferulic acid	69:80	Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer's disease: A preclinical evidence.					
34022704	6	95	theme	drug	1106:1109	arg1	crystallinity					1111:1123	drug crystallinity	1106:1123	drug crystallinity	1106:1123	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	10	96	theme	potential	1922:1930	arg1	compliance					1911:1920	improved patient compliance potential	1894:1930	improved patient compliance potential	1894:1930	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	8	97	theme	Substantial	1382:1392	arg1	improvement					1394:1404	Substantial improvement	1382:1404	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain	1382:1601	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	3	98	theme	chitosan-coated	522:536	arg1	SLNs					565:568	SLNs	565:568	SLNs	565:568	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	3	98	theme	chitosan-coated	522:536	arg1	nanoparticles					550:562	chitosan-coated solid lipid nanoparticles	522:562	chitosan-coated solid lipid nanoparticles (SLNs)	522:569	The current studies, therefore, were undertaken to systematically develop chitosan-coated solid lipid nanoparticles (SLNs) using QbD paradigms for improved efficacy of FA in the management of Alzheimer's disease (AD).					
34022704	10	99	theme	patient	1903:1909	arg1	compliance					1911:1920	improved patient compliance potential	1894:1930	improved patient compliance potential	1894:1930	Overall, these preclinical findings successfully demonstrate improved anti-AD efficacy, superior nasal mucoadhesion and permeation, extended drug release, improved patient compliance potential, safety and robustness of the developed lipidic nanoconstructs of FA through intranasal route.					
34022704	4	100	theme	Central	780:786	arg1	Design					798:803	a 32 Central Composite Design	775:803	a 32 Central Composite Design (CCD)	775:809	SLNs of FA were formulated employing Compritol as lipid and polysorbate 80 as surfactant and optimised using a 32 Central Composite Design (CCD).					
34022704	4	100	theme	Central	780:786	arg1	CCD					806:808	CCD	806:808	CCD	806:808	SLNs of FA were formulated employing Compritol as lipid and polysorbate 80 as surfactant and optimised using a 32 Central Composite Design (CCD).					
34022704	1	101	theme	distinctive	218:228	arg1	promise					230:236	distinctive promise	218:236	distinctive promise in neurodegenerative disorders	218:267	Ferulic acid (FA) is a ubiquitous natural plant bioactive with distinctive promise in neurodegenerative disorders.					
34022704	9	102	theme	Histopathological	1636:1652	arg1	images					1654:1659	Histopathological images	1636:1659	Histopathological images of different rat organs	1636:1683	Histopathological images of different rat organs showed no perceptible change(s) in tissue morphology.					
34022704	6	103	theme	construed	1076:1084	arg1	loss					1098:1101	PXRD construed significant loss	1071:1101	PXRD construed significant loss of drug crystallinity	1071:1123	FTIR and DSC studies verified the compatibility of FA with formulation excipients, PXRD construed significant loss of drug crystallinity, while FESEM depicted existence of uniform spherical nanoparticles with little aggregation.					
34022704	8	104	theme	AD-induced	1619:1628	arg1	rats					1630:1633	AD-induced rats	1619:1633	AD-induced rats	1619:1633	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34022704	8	105	dep	together	1510:1517	arg1	with					1519:1522	with	1519:1522	with	1519:1522	Substantial improvement with SLNs in cognitive ability through the reduction in escape latency time during behavioural studies, together with significant improvement in various biochemical parameters and body weight gain was observed in AD-induced rats.					
34970318	6	0	theme	LAB	1189:1191	arg1	growth					1162:1167	the growth	1158:1167	the growth of Bifidobacterium, LAB, and total bacteria	1158:1211	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	3	1	theme	bacteria	560:567	arg1	numbers					517:523	numbers	517:523	numbers of Bifidobacterium and lactic acid bacteria	517:567	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	6	2	theme	fungi	1263:1267	arg1	proliferation					1229:1241	the proliferation	1225:1241	the proliferation of Enterococcus and fungi	1225:1267	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	3	3	theme	lactic	548:553	arg1	bacteria					560:567	Bifidobacterium and lactic acid bacteria	528:567	bacteria	560:567	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	4	4	dep	Limosilactobacillus	805:823	arg1	reuteri					825:831	reuteri	825:831	reuteri	825:831	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	6	5	theme	Enterococcus	1246:1257	arg1	proliferation					1229:1241	the proliferation	1225:1241	the proliferation of Enterococcus and fungi	1225:1267	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	3	6	theme	pH	590:591	arg1	value					593:597	the pH value	586:597	the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose	586:689	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	9	7	theme	effective	1585:1593	arg1	ability					1595:1601	The more effective ability	1576:1601	The more effective ability	1576:1601	The more effective ability was observed with IMO combined with L. helveticus KM7.					
34970318	8	8	theme	fermentation	1557:1568	arg1	time					1570:1573	fermentation time	1557:1573	fermentation time	1557:1573	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	10	9	theme	certain	1763:1769	arg1	strains					1784:1790	certain lactobacilli strains	1763:1790	certain lactobacilli strains	1763:1790	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	4	10	theme	A.	736:737	arg1	gut					746:748	A. cerana gut	736:748	A. cerana gut	736:748	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	5	11	theme	pronounced	966:975	arg1	impact					977:982	The most pronounced impact	957:982	The most pronounced impact	957:982	The most pronounced impact was observed with isomalto-oligosaccharides.					
34970318	6	12	theme	isomalto-oligosaccharides	1093:1117	arg1	combination					1078:1088	the combination	1074:1088	the combination of isomalto-oligosaccharides with both lactobacilli strains	1074:1148	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	7	13	theme	lactobacilli	1445:1456	arg1	strains					1458:1464	lactobacilli strains	1445:1464	lactobacilli strains	1445:1464	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	0	14	theme	Apis	49:52	arg1	Microbiota					65:74	Apis cerana Gut Microbiota	49:74	Apis cerana Gut Microbiota	49:74	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	3	15	theme	broth	624:628	arg1	value					593:597	the pH value	586:597	the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose	586:689	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	9	16	theme	L.	1639:1640	arg1	helveticus					1642:1651	L. helveticus KM7	1639:1655	L. helveticus KM7	1639:1655	The more effective ability was observed with IMO combined with L. helveticus KM7.					
34970318	0	17	from	Effects	9:15	arg1	Microbiota					65:74	Apis cerana Gut Microbiota	49:74	Apis cerana Gut Microbiota	49:74	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	4	18	theme	potential	702:710	arg1	probiotics					712:721	two potential probiotics	698:721	two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4	698:835	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	0	19	theme	Gut	61:63	arg1	Microbiota					65:74	Apis cerana Gut Microbiota	49:74	Apis cerana Gut Microbiota	49:74	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	7	20	located	observed	1336:1343	arg2	activity					1323:1330	the altered metabolic activity	1301:1330	the altered metabolic activity	1301:1330	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	7	20	located	observed	1336:1343	arg1	culture					1371:1377	in vitro culture	1362:1377	in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains	1362:1464	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	7	20	located	observed	1336:1343	arg2	pH					1356:1357	lowered pH	1348:1357	lowered pH	1348:1357	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	1	21	theme	selected	133:140	arg1	prebiotics					142:151	the selected prebiotics	129:151	the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains	129:228	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	1	22	dep	in	109:110	arg1	vitro					112:116	vitro	112:116	vitro	112:116	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	1	23	from	effects	118:124	arg1	composition					247:257	the microbial composition	233:257	the microbial composition of Apis cerana gut microbiota and acid production	233:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	0	24	theme	cerana	54:59	arg1	Microbiota					65:74	Apis cerana Gut Microbiota	49:74	Apis cerana Gut Microbiota	49:74	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	0	25	theme	In	0:1	arg1	Effects					9:15	In vitro Effects	0:15	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.	0:75	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	4	26	theme	different	753:761	arg1	LP4					833:835	Limosilactobacillus reuteri LP4	805:835	Limosilactobacillus reuteri LP4	805:835	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	4	26	theme	different	753:761	arg1	dosages					763:769	different dosages	753:769	different dosages	753:769	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	4	26	theme	different	753:761	arg1	helveticus					786:795	Lactobacillus helveticus KM7	772:799	Lactobacillus helveticus KM7	772:799	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	1	27	theme	cerana	267:272	arg1	microbiota					278:287	Apis cerana gut microbiota	262:287	Apis cerana gut microbiota	262:287	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	7	28	theme	microbiota	1386:1395	arg1	culture					1371:1377	in vitro culture	1362:1377	in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains	1362:1464	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	4	29	dep	A.	917:918	arg1	cerana					920:925	cerana	920:925	cerana	920:925	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	1	30	theme	microbiota	278:287	arg1	composition					247:257	the microbial composition	233:257	the microbial composition of Apis cerana gut microbiota and acid production	233:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	0	31	theme	Prebiotics	20:29	arg1	Effects					9:15	In vitro Effects	0:15	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.	0:75	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	1	32	with	combination	167:177	arg1	strains					222:228	two potential probiotic Lactobacillus strains	184:228	two potential probiotic Lactobacillus strains	184:228	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	1	33	theme	acid	293:296	arg1	production					298:307	acid production	293:307	acid production	293:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	8	34	theme	Lactobacillus	1531:1543	arg1	strains					1545:1551	Lactobacillus strains	1531:1551	Lactobacillus strains	1531:1551	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	3	35	dep	A.	646:647	arg1	cerana					649:654	cerana	649:654	cerana	649:654	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	6	36	theme	total	1198:1202	arg1	bacteria					1204:1211	total bacteria	1198:1211	total bacteria	1198:1211	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	1	37	theme	Lactobacillus	208:220	arg1	strains					222:228	two potential probiotic Lactobacillus strains	184:228	two potential probiotic Lactobacillus strains	184:228	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	0	38	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	7	39	theme	lowered	1348:1354	arg1	pH					1356:1357	lowered pH	1348:1357	lowered pH	1348:1357	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	7	39	theme	lowered	1348:1354	arg1	activity					1323:1330	the altered metabolic activity	1301:1330	the altered metabolic activity	1301:1330	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	10	40	dep	A.	1795:1796	arg1	cerana					1798:1803	cerana	1798:1803	cerana	1798:1803	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	4	41	attach	derived	723:729	arg2	probiotics					712:721	two potential probiotics	698:721	two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4	698:835	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	4	41	attach	derived	723:729	arg1	gut					746:748	A. cerana gut	736:748	A. cerana gut	736:748	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	3	42	theme	acid	555:558	arg1	bacteria					560:567	Bifidobacterium and lactic acid bacteria	528:567	bacteria	560:567	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	1	43	theme	microbial	237:245	arg1	composition					247:257	the microbial composition	233:257	the microbial composition of Apis cerana gut microbiota and acid production	233:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	4	44	theme	A.	917:918	arg1	microbiota					931:940	A. cerana gut microbiota	917:940	A. cerana gut microbiota	917:940	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	1	45	theme	prebiotics	142:151	arg1	effects					118:124	in vitro effects	109:124	in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production	109:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	0	46	theme	Synbiotics	35:44	arg1	Effects					9:15	In vitro Effects	0:15	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.	0:75	In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota.					
34970318	7	47	theme	altered	1305:1311	arg1	pH					1356:1357	lowered pH	1348:1357	lowered pH	1348:1357	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	7	47	theme	altered	1305:1311	arg1	activity					1323:1330	the altered metabolic activity	1301:1330	the altered metabolic activity	1301:1330	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	3	48	dep	in	602:603	arg1	vitro					605:609	vitro	605:609	vitro	605:609	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	4	49	theme	fermentation	882:893	arg1	broth					895:899	fermentation broth	882:899	fermentation broth inoculated with A. cerana gut microbiota, respectively	882:954	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	10	50	theme	potential	1724:1732	arg1	prebiotic					1734:1742	a potential prebiotic	1722:1742	a potential prebiotic	1722:1742	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	10	50	theme	potential	1724:1732	arg1	isomalto-oligosaccharides					1687:1711	isomalto-oligosaccharides	1687:1711	isomalto-oligosaccharides	1687:1711	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	8	51	theme	time	1570:1573	arg1	concentration					1514:1526	concentration	1514:1526	concentration	1514:1526	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	8	51	theme	time	1570:1573	arg1	types					1504:1508	types	1504:1508	types	1504:1508	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	7	52	with	Consistent	1270:1279	arg1	results					1292:1298	these results	1286:1298	these results	1286:1298	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	10	53	theme	lactobacilli	1771:1782	arg1	strains					1784:1790	certain lactobacilli strains	1763:1790	certain lactobacilli strains	1763:1790	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	3	54	theme	in	602:603	arg1	broth					624:628	in vitro fermentation broth	602:628	in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose	602:689	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	6	55	with	combination	1078:1088	arg1	strains					1142:1148	both lactobacilli strains	1124:1148	both lactobacilli strains	1124:1148	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	1	56	theme	production	298:307	arg1	composition					247:257	the microbial composition	233:257	the microbial composition of Apis cerana gut microbiota and acid production	233:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	4	57	theme	gut	927:929	arg1	microbiota					931:940	A. cerana gut microbiota	917:940	A. cerana gut microbiota	917:940	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	1	58	theme	in	109:110	arg1	effects					118:124	in vitro effects	109:124	in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production	109:307	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	7	59	theme	metabolic	1313:1321	arg1	pH					1356:1357	lowered pH	1348:1357	lowered pH	1348:1357	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	7	59	theme	metabolic	1313:1321	arg1	activity					1323:1330	the altered metabolic activity	1301:1330	the altered metabolic activity	1301:1330	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	3	60	theme	fermentation	611:622	arg1	broth					624:628	in vitro fermentation broth	602:628	in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose	602:689	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	1	61	theme	potential	188:196	arg1	strains					222:228	two potential probiotic Lactobacillus strains	184:228	two potential probiotic Lactobacillus strains	184:228	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	8	62	dep	types	1504:1508	arg1	the					1500:1502	the	1500:1502	the	1500:1502	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	7	63	dep	in	1362:1363	arg1	vitro					1365:1369	vitro	1365:1369	vitro	1365:1369	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	6	64	theme	Bifidobacterium	1172:1186	arg1	growth					1162:1167	the growth	1158:1167	the growth of Bifidobacterium, LAB, and total bacteria	1158:1211	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	4	65	theme	Lactobacillus	772:784	arg1	dosages					763:769	different dosages	753:769	different dosages	753:769	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	4	65	theme	Lactobacillus	772:784	arg1	helveticus					786:795	Lactobacillus helveticus KM7	772:799	Lactobacillus helveticus KM7	772:799	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	10	66	theme	A.	1795:1796	arg1	microbiota					1809:1818	A. cerana gut microbiota	1795:1818	A. cerana gut microbiota	1795:1818	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	4	67	from	isomalto-oligosaccharides	853:877	arg1	broth					895:899	fermentation broth	882:899	fermentation broth inoculated with A. cerana gut microbiota, respectively	882:954	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	4	68	dep	A.	736:737	arg1	cerana					739:744	cerana	739:744	cerana	739:744	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	8	69	theme	symbiotic	1471:1479	arg1	impact					1481:1486	The symbiotic impact	1467:1486	The symbiotic impact	1467:1486	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	1	70	theme	Apis	262:265	arg1	microbiota					278:287	Apis cerana gut microbiota	262:287	Apis cerana gut microbiota	262:287	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	7	71	theme	gut	1382:1384	arg1	microbiota					1386:1395	gut microbiota	1382:1395	gut microbiota	1382:1395	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	3	72	theme	gut	656:658	arg1	microbiota					660:669	A. cerana gut microbiota	646:669	A. cerana gut microbiota	646:669	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	1	73	theme	gut	274:276	arg1	microbiota					278:287	Apis cerana gut microbiota	262:287	Apis cerana gut microbiota	262:287	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	6	74	theme	lactobacilli	1129:1140	arg1	strains					1142:1148	both lactobacilli strains	1124:1148	both lactobacilli strains	1124:1148	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	3	75	theme	A.	646:647	arg1	microbiota					660:669	A. cerana gut microbiota	646:669	A. cerana gut microbiota	646:669	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	10	76	theme	gut	1805:1807	arg1	microbiota					1809:1818	A. cerana gut microbiota	1795:1818	A. cerana gut microbiota	1795:1818	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	7	77	theme	in	1362:1363	arg1	culture					1371:1377	in vitro culture	1362:1377	in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains	1362:1464	Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains.					
34970318	2	78	theme	carbon	452:457	arg1	source					459:464	the carbon source	448:464	the carbon source	448:464	Four prebiotics, inulin, fructo-oligosaccharides, xylo-oligosaccharides, and isomalto-oligosaccharides were chosen, and glucose served as the carbon source.					
34970318	2	78	theme	carbon	452:457	arg1	glucose					430:436	glucose	430:436	glucose	430:436	Four prebiotics, inulin, fructo-oligosaccharides, xylo-oligosaccharides, and isomalto-oligosaccharides were chosen, and glucose served as the carbon source.					
34970318	3	79	theme	prebiotics	496:505	arg1	Supplementation					467:481	Supplementation	467:481	Supplementation of this four prebiotics	467:505	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34970318	8	80	theme	strains	1545:1551	arg1	concentration					1514:1526	concentration	1514:1526	concentration	1514:1526	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	8	80	theme	strains	1545:1551	arg1	types					1504:1508	types	1504:1508	types	1504:1508	The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time.					
34970318	6	81	theme	bacteria	1204:1211	arg1	growth					1162:1167	the growth	1158:1167	the growth of Bifidobacterium, LAB, and total bacteria	1158:1211	Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi.					
34970318	1	82	theme	probiotic	198:206	arg1	strains					222:228	two potential probiotic Lactobacillus strains	184:228	two potential probiotic Lactobacillus strains	184:228	This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production.					
34970318	10	83	with	symbiotic	1748:1756	arg1	strains					1784:1790	certain lactobacilli strains	1763:1790	certain lactobacilli strains	1763:1790	These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.					
34970318	4	84	theme	Limosilactobacillus	805:823	arg1	dosages					763:769	different dosages	753:769	different dosages	753:769	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	4	84	theme	Limosilactobacillus	805:823	arg1	LP4					833:835	Limosilactobacillus reuteri LP4	805:835	Limosilactobacillus reuteri LP4	805:835	Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively.					
34970318	3	85	theme	Bifidobacterium	528:542	arg1	bacteria					560:567	Bifidobacterium and lactic acid bacteria	528:567	bacteria	560:567	Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose.					
34705551	7	0	theme	proteins	1484:1491	arg1	levels					1432:1437	the mRNA expression levels	1412:1437	the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa	1412:1513	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	5	1	theme	mucosal	1092:1098	arg1	bacteria					1100:1107	the colonic mucosal bacteria	1080:1107	the colonic mucosal bacteria	1080:1107	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	14	2	theme	gut	2683:2685	arg1	dysfunction					2687:2697	intestinal ecosystem dysbiosis and gut dysfunction	2648:2697	dysfunction	2687:2697	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	10	3	theme	weaning	1900:1906	arg1	stresses					1908:1915	weaning stresses	1900:1915	weaning stresses of piglets	1900:1926	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	6	4	theme	acid	1263:1266	arg1	producers					1275:1283	short-chain fatty acid (SCFA) producers	1245:1283	short-chain fatty acid (SCFA) producers in different colonic niches	1245:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	7	5	theme	mRNA	1416:1419	arg1	levels					1432:1437	the mRNA expression levels	1412:1437	the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa	1412:1513	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	13	6	theme	MyD88-NF-κB	2372:2382	arg1	levels					2362:2367	the expression levels	2347:2367	the expression levels of MyD88-NF-κB	2347:2382	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	13	6	theme	MyD88-NF-κB	2372:2382	arg1	cytokines					2408:2416	the proinflammatory cytokines	2388:2416	the proinflammatory cytokines	2388:2416	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	10	7	theme	economic	1941:1948	arg1	losses					1950:1955	economic losses	1941:1955	economic losses in the modern swine industry	1941:1984	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	6	8	theme	short-chain	1245:1255	arg1	SCFA					1269:1272	SCFA	1269:1272	SCFA	1269:1272	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	6	8	theme	short-chain	1245:1255	arg1	acid					1263:1266	short-chain fatty acid	1245:1266	short-chain fatty acid (SCFA) producers in different colonic niches	1245:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	6	9	from	abundances	1231:1240	arg1	niches					1306:1311	different colonic niches	1288:1311	different colonic niches	1288:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	7	10	theme	recognition	1450:1460	arg1	receptors					1462:1470	pattern recognition receptors	1442:1470	pattern recognition receptors	1442:1470	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	3	11	theme	colonic	705:711	arg1	microbiota					713:722	colonic microbiota	705:722	colonic microbiota	705:722	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	9	12	theme	PWG	1809:1811	arg1	effect					1791:1796	the combined effect	1778:1796	the combined effect of ELG and PWG	1778:1811	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	1	13	theme	galacto-oligosaccharides	194:217	arg1	intervention					225:236	the early-life galacto-oligosaccharides (GOS) intervention	179:236	the early-life galacto-oligosaccharides (GOS) intervention	179:236	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	7	14	from	levels	1432:1437	arg1	mucosa					1508:1513	the colonic mucosa	1496:1513	the colonic mucosa	1496:1513	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	9	15	theme	expression	1673:1682	arg1	levels					1684:1689	the expression levels	1669:1689	the expression levels of MyD88-NF-κB signaling	1669:1714	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	9	16	theme	proinflammatory	1724:1738	arg1	cytokines					1740:1748	the proinflammatory cytokines	1720:1748	the proinflammatory cytokines	1720:1748	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	5	17	dep	niches	1016:1021	arg1	digesta					1035:1041	digesta	1035:1041	digesta	1035:1041	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	5	17	dep	niches	1016:1021	arg1	mucosa					1024:1029	mucosa	1024:1029	mucosa	1024:1029	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	5	17	dep	niches	1016:1021	arg1	niches					1016:1021	different colonic niches	998:1021	different colonic niches (mucosa and digesta)	998:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	3	18	theme	weaning	748:754	arg1	piglets					756:762	weaning piglets	748:762	weaning piglets	748:762	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	13	19	theme	ELG	2321:2323	arg1	effect					2311:2316	the combined effect	2298:2316	the combined effect of ELG and PWG intervention	2298:2344	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	14	20	theme	ecosystem	2659:2667	arg1	dysbiosis					2669:2677	intestinal ecosystem dysbiosis and gut dysfunction	2648:2697	dysbiosis	2669:2677	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	1	21	from	composition	299:309	arg1	piglets					323:329	suckling piglets	314:329	suckling piglets	314:329	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	9	22	theme	signaling	1706:1714	arg1	Meanwhile					1658:1666	Meanwhile	1658:1666	Meanwhile	1658:1666	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	9	22	theme	signaling	1706:1714	arg1	cytokines					1740:1748	the proinflammatory cytokines	1720:1748	the proinflammatory cytokines	1720:1748	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	9	22	theme	signaling	1706:1714	arg1	levels					1684:1689	the expression levels	1669:1689	the expression levels of MyD88-NF-κB signaling	1669:1714	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	6	23	theme	different	1288:1296	arg1	niches					1306:1311	different colonic niches	1288:1311	different colonic niches	1288:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	2	24	theme	weaning	575:581	arg1	piglets					583:589	weaning piglets	575:589	weaning piglets	575:589	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	6	25	theme	producers	1275:1283	arg1	abundances					1231:1240	the relative abundances	1218:1240	the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches	1218:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	4	26	theme	colonic	911:917	arg1	indexes					919:925	colonic indexes	911:925	colonic indexes	911:925	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	6	27	dep	ELG	1183:1185	arg1	the					1179:1181	the	1179:1181	the	1179:1181	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	14	28	theme	new	2589:2591	arg1	insights					2593:2600	new insights	2589:2600	new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition	2589:2742	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	6	29	theme	colonic	1359:1365	arg1	digesta					1367:1373	colonic digesta	1359:1373	colonic digesta	1359:1373	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	5	30	theme	bacteria	1100:1107	arg1	α-diversity					1065:1075	the α-diversity	1061:1075	the α-diversity of the colonic mucosal bacteria during PWG intervention	1061:1131	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	0	31	theme	Bacterial	99:107	arg1	Composition					109:119	Colonic Bacterial Composition	91:119	Colonic Bacterial Composition	91:119	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	5	32	theme	PWG	1116:1118	arg1	intervention					1120:1131	PWG intervention	1116:1131	PWG intervention	1116:1131	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	10	33	from	losses	1950:1955	arg1	industry					1977:1984	the modern swine industry	1960:1984	the modern swine industry	1960:1984	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	13	34	theme	weaning	2540:2546	arg1	piglets					2548:2554	weaning piglets	2540:2554	weaning piglets	2540:2554	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	9	35	theme	combined	1782:1789	arg1	effect					1791:1796	the combined effect	1778:1796	the combined effect of ELG and PWG	1778:1811	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	12	36	theme	weaning	2264:2270	arg1	piglets					2272:2278	the weaning piglets	2260:2278	the weaning piglets	2260:2278	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	12	37	theme	different	2208:2216	arg1	niches					2226:2231	different colonic niches	2208:2231	different colonic niches	2208:2231	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	14	38	from	dysfunction	2687:2697	arg1	piglets					2706:2712	the piglets	2702:2712	the piglets	2702:2712	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	6	39	theme	total	1331:1335	arg1	concentration					1342:1354	the total SCFA concentration	1327:1354	the total SCFA concentration	1327:1354	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	12	40	theme	niches	2226:2231	arg1	composition					2193:2203	the bacterial composition	2179:2203	the bacterial composition of different colonic niches	2179:2231	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	5	41	theme	colonic	1084:1090	arg1	bacteria					1100:1107	the colonic mucosal bacteria	1080:1107	the colonic mucosal bacteria	1080:1107	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	13	42	theme	piglets	2548:2554	arg1	colon					2531:2535	the colon	2527:2535	the colon of weaning piglets	2527:2554	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	5	43	theme	bacteria	976:983	arg1	sequencing					954:963	rRNA gene MiSeq sequencing	938:963	rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta)	938:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	2	44	theme	ELG	466:468	arg1	intervention					496:507	the combined ELG and postweaning GOS (PWG) intervention	453:507	the combined ELG and postweaning GOS (PWG) intervention	453:507	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	12	45	theme	colonic	2240:2246	arg1	function					2248:2255	colonic function	2240:2255	colonic function in the weaning piglets	2240:2278	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	2	46	theme	long	400:403	arg1	influence					405:413	a long influence	398:413	a long influence	398:413	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	0	47	theme	Differential	0:11	arg1	Effects					13:19	Differential Effects	0:19	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.	0:152	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	5	48	theme	gene	943:946	arg1	sequencing					954:963	rRNA gene MiSeq sequencing	938:963	rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta)	938:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	10	49	theme	ecosystem	1859:1867	arg1	disorders					1838:1846	the disorders	1834:1846	the disorders of the gut ecosystem	1834:1867	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	13	50	theme	proinflammatory	2392:2406	arg1	cytokines					2408:2416	the proinflammatory cytokines	2388:2416	the proinflammatory cytokines	2388:2416	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	3	51	theme	differential	648:659	arg1	effects					661:667	the differential effects	644:667	the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets	644:762	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	2	52	theme	colonic	422:428	arg1	microbiota					430:439	the colonic microbiota	418:439	the colonic microbiota	418:439	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	13	53	theme	combined	2302:2309	arg1	effect					2311:2316	the combined effect	2298:2316	the combined effect of ELG and PWG intervention	2298:2344	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	13	54	theme	expression	2351:2360	arg1	levels					2362:2367	the expression levels	2347:2367	the expression levels of MyD88-NF-κB	2347:2382	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	14	55	theme	weaning	2725:2731	arg1	transition					2733:2742	the weaning transition	2721:2742	the weaning transition	2721:2742	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	2	56	theme	postweaning	474:484	arg1	GOS					486:488	postweaning GOS	474:488	postweaning GOS	474:488	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	8	57	theme	ELG	1548:1550	arg1	effect					1538:1543	the combined effect	1525:1543	the combined effect of ELG and PWG	1525:1558	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	2	58	theme	ELG	369:371	arg1	intervention					374:385	the early-life GOS (ELG) intervention	349:385	the early-life GOS (ELG) intervention	349:385	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	0	59	theme	Galacto-oligosaccharide	51:73	arg1	Intervention					75:86	Early-Life and Postweaning Galacto-oligosaccharide Intervention	24:86	Early-Life and Postweaning Galacto-oligosaccharide Intervention	24:86	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	7	60	theme	colonic	1500:1506	arg1	mucosa					1508:1513	the colonic mucosa	1496:1513	the colonic mucosa	1496:1513	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	8	61	theme	PWG	1556:1558	arg1	effect					1538:1543	the combined effect	1525:1543	the combined effect of ELG and PWG	1525:1558	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	3	62	theme	ELG	676:678	arg1	interventions					688:700	the ELG and PWG interventions	672:700	interventions	688:700	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	12	63	theme	ELG	2125:2127	arg1	interventions					2137:2149	the ELG and PWG interventions	2121:2149	the ELG and PWG interventions	2121:2149	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	2	64	theme	GOS	364:366	arg1	intervention					374:385	the early-life GOS (ELG) intervention	349:385	the early-life GOS (ELG) intervention	349:385	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	8	65	theme	butyrate	1634:1641	arg1	concentration					1643:1655	the butyrate concentration	1630:1655	the butyrate concentration	1630:1655	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	13	66	theme	PWG	2329:2331	arg1	intervention					2333:2344	PWG intervention	2329:2344	PWG intervention	2329:2344	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	3	67	from	effects	661:667	arg1	microbiota					713:722	colonic microbiota	705:722	colonic microbiota	705:722	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	3	67	from	effects	661:667	arg1	function					736:743	colonic function	728:743	colonic function	728:743	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	12	68	theme	PWG	2133:2135	arg1	interventions					2137:2149	the ELG and PWG interventions	2121:2149	the ELG and PWG interventions	2121:2149	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	7	69	theme	barrier	1476:1482	arg1	proteins					1484:1491	barrier proteins	1476:1491	barrier proteins	1476:1491	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	5	70	theme	colonic	1008:1014	arg1	digesta					1035:1041	digesta	1035:1041	digesta	1035:1041	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	5	70	theme	colonic	1008:1014	arg1	mucosa					1024:1029	mucosa	1024:1029	mucosa	1024:1029	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	5	70	theme	colonic	1008:1014	arg1	niches					1016:1021	different colonic niches	998:1021	different colonic niches (mucosa and digesta)	998:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	8	71	theme	SCFA	1589:1592	arg1	enrichment					1603:1612	colonic SCFA producer enrichment	1581:1612	colonic SCFA producer enrichment	1581:1612	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	7	72	theme	receptors	1462:1470	arg1	levels					1432:1437	the mRNA expression levels	1412:1437	the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa	1412:1513	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	4	73	theme	weaning	858:864	arg1	piglets					866:872	weaning piglets	858:872	weaning piglets	858:872	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	3	74	theme	PWG	684:686	arg1	interventions					688:700	the ELG and PWG interventions	672:700	interventions	688:700	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	6	75	theme	fatty	1257:1261	arg1	SCFA					1269:1272	SCFA	1269:1272	SCFA	1269:1272	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	6	75	theme	fatty	1257:1261	arg1	acid					1263:1266	short-chain fatty acid	1245:1266	short-chain fatty acid (SCFA) producers in different colonic niches	1245:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	1	76	theme	bacterial	289:297	arg1	composition					299:309	the bacterial composition	285:309	the bacterial composition in suckling piglets	285:329	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	7	77	theme	pattern	1442:1448	arg1	receptors					1462:1470	pattern recognition receptors	1442:1470	pattern recognition receptors	1442:1470	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	7	78	theme	expression	1421:1430	arg1	levels					1432:1437	the mRNA expression levels	1412:1437	the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa	1412:1513	These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa.					
34705551	3	79	theme	colonic	728:734	arg1	function					736:743	colonic function	728:743	colonic function	728:743	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	13	80	theme	microbial	2494:2502	arg1	concentrations					2444:2457	increasing concentrations	2433:2457	increasing concentrations	2433:2457	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	13	80	theme	microbial	2494:2502	arg1	metabolite					2504:2513	an important microbial metabolite	2481:2513	an important microbial metabolite involved in the colon of weaning piglets	2481:2554	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	1	81	theme	early-life	183:192	arg1	GOS					220:222	GOS	220:222	GOS	220:222	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	1	81	theme	early-life	183:192	arg1	galacto-oligosaccharides					194:217	early-life galacto-oligosaccharides	183:217	the early-life galacto-oligosaccharides (GOS) intervention	179:236	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	10	82	theme	piglets	1920:1926	arg1	stresses					1908:1915	weaning stresses	1900:1915	weaning stresses of piglets	1900:1926	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	6	83	theme	relative	1222:1229	arg1	abundances					1231:1240	the relative abundances	1218:1240	the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches	1218:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	10	84	theme	modern	1964:1969	arg1	industry					1977:1984	the modern swine industry	1960:1984	the modern swine industry	1960:1984	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	4	85	from	piglets	866:872	arg1	frequency					833:841	the frequency	829:841	the frequency of diarrhea in weaning piglets	829:872	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	4	86	theme	diarrhea	846:853	arg1	frequency					833:841	the frequency	829:841	the frequency of diarrhea in weaning piglets	829:872	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	14	87	from	dysbiosis	2669:2677	arg1	piglets					2706:2712	the piglets	2702:2712	the piglets	2702:2712	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	3	88	theme	piglets	756:762	arg1	microbiota					713:722	colonic microbiota	705:722	colonic microbiota	705:722	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	3	88	theme	piglets	756:762	arg1	function					736:743	colonic function	728:743	colonic function	728:743	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	13	89	theme	intervention	2333:2344	arg1	effect					2311:2316	the combined effect	2298:2316	the combined effect of ELG and PWG intervention	2298:2344	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	14	90	theme	intestinal	2648:2657	arg1	dysbiosis					2669:2677	intestinal ecosystem dysbiosis and gut dysfunction	2648:2697	dysbiosis	2669:2677	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	2	91	theme	colonic	557:563	arg1	health					565:570	colonic health	557:570	colonic health	557:570	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	0	92	from	Composition	109:119	arg1	Piglets					145:151	Weaning Piglets	137:151	Weaning Piglets	137:151	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	6	93	theme	colonic	1298:1304	arg1	niches					1306:1311	different colonic niches	1288:1311	different colonic niches	1288:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	12	94	theme	present	2085:2091	arg1	study					2093:2097	the present study	2081:2097	the present study	2081:2097	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	12	95	from	function	2248:2255	arg1	piglets					2272:2278	the weaning piglets	2260:2278	the weaning piglets	2260:2278	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	14	96	theme	nutritional	2607:2617	arg1	interventions					2619:2631	nutritional interventions	2607:2631	nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition	2607:2742	These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.					
34705551	4	97	theme	PWG	884:886	arg1	intervention					888:899	the PWG intervention	880:899	the PWG intervention	880:899	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	10	98	theme	swine	1971:1975	arg1	industry					1977:1984	the modern swine industry	1960:1984	the modern swine industry	1960:1984	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	13	99	theme	butyrate	2462:2469	arg1	concentrations					2444:2457	increasing concentrations	2433:2457	increasing concentrations	2433:2457	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	13	99	theme	butyrate	2462:2469	arg1	metabolite					2504:2513	an important microbial metabolite	2481:2513	an important microbial metabolite involved in the colon of weaning piglets	2481:2554	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	11	100	theme	weaning	2058:2064	arg1	transition					2066:2075	the weaning transition	2054:2075	the weaning transition	2054:2075	To this end, prebiotics have been often added to their diet during the weaning transition.					
34705551	0	101	from	Function	125:132	arg1	Piglets					145:151	Weaning Piglets	137:151	Weaning Piglets	137:151	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	0	102	theme	Colonic	91:97	arg1	Composition					109:119	Colonic Bacterial Composition	91:119	Colonic Bacterial Composition	91:119	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	9	103	theme	MyD88-NF-κB	1694:1704	arg1	signaling					1706:1714	MyD88-NF-κB signaling	1694:1714	MyD88-NF-κB signaling	1694:1714	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	0	104	from	Effects	13:19	arg1	Function					125:132	Function	125:132	Function	125:132	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	0	104	from	Effects	13:19	arg1	Composition					109:119	Colonic Bacterial Composition	91:119	Colonic Bacterial Composition	91:119	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	6	105	dep	interventions	1195:1207	arg1	increased					1317:1325	increased	1317:1325	increased the total SCFA concentration in colonic digesta	1317:1373	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	6	105	dep	interventions	1195:1207	arg1	enriched					1209:1216	enriched	1209:1216	enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches	1209:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	6	106	theme	SCFA	1337:1340	arg1	concentration					1342:1354	the total SCFA concentration	1327:1354	the total SCFA concentration	1327:1354	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	2	107	theme	interacting	523:533	arg1	effect					535:540	an interacting effect	520:540	an interacting effect	520:540	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	5	108	from	increase	1049:1056	arg1	α-diversity					1065:1075	the α-diversity	1061:1075	the α-diversity of the colonic mucosal bacteria during PWG intervention	1061:1131	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	4	109	from	diarrhea	846:853	arg1	piglets					866:872	weaning piglets	858:872	weaning piglets	858:872	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	5	110	theme	gut	972:974	arg1	bacteria					976:983	the gut bacteria	968:983	the gut bacteria belonging to different colonic niches (mucosa and digesta)	968:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	9	111	theme	ELG	1801:1803	arg1	effect					1791:1796	the combined effect	1778:1796	the combined effect of ELG and PWG	1778:1811	Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG.					
34705551	12	112	theme	colonic	2218:2224	arg1	niches					2226:2231	different colonic niches	2208:2231	different colonic niches	2208:2231	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	0	113	theme	Weaning	137:143	arg1	Piglets					145:151	Weaning Piglets	137:151	Weaning Piglets	137:151	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	4	114	from	frequency	833:841	arg1	piglets					866:872	weaning piglets	858:872	weaning piglets	858:872	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	5	115	theme	rRNA	938:941	arg1	sequencing					954:963	rRNA gene MiSeq sequencing	938:963	rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta)	938:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	2	116	theme	combined	457:464	arg1	PWG					491:493	PWG	491:493	PWG	491:493	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	2	116	theme	combined	457:464	arg1	ELG					466:468	combined ELG	457:468	combined ELG	457:468	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	8	117	theme	combined	1529:1536	arg1	effect					1538:1543	the combined effect	1525:1543	the combined effect of ELG and PWG	1525:1558	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	0	118	theme	Early-Life	24:33	arg1	Intervention					75:86	Early-Life and Postweaning Galacto-oligosaccharide Intervention	24:86	Early-Life and Postweaning Galacto-oligosaccharide Intervention	24:86	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	1	119	theme	suckling	314:321	arg1	piglets					323:329	suckling piglets	314:329	suckling piglets	314:329	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	5	120	theme	MiSeq	948:952	arg1	sequencing					954:963	rRNA gene MiSeq sequencing	938:963	rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta)	938:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	2	121	contain	have	393:396	arg1	intervention					374:385	the early-life GOS (ELG) intervention	349:385	the early-life GOS (ELG) intervention	349:385	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	2	121	contain	have	393:396	arg2	influence					405:413	a long influence	398:413	a long influence	398:413	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	12	122	theme	bacterial	2183:2191	arg1	composition					2193:2203	the bacterial composition	2179:2203	the bacterial composition of different colonic niches	2179:2231	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	0	123	theme	Postweaning	39:49	arg1	Intervention					75:86	Early-Life and Postweaning Galacto-oligosaccharide Intervention	24:86	Early-Life and Postweaning Galacto-oligosaccharide Intervention	24:86	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	6	124	from	niches	1306:1311	arg1	abundances					1231:1240	the relative abundances	1218:1240	the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches	1218:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	2	125	theme	GOS	486:488	arg1	intervention					496:507	the combined ELG and postweaning GOS (PWG) intervention	453:507	the combined ELG and postweaning GOS (PWG) intervention	453:507	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	10	126	theme	gut	1855:1857	arg1	ecosystem					1859:1867	the gut ecosystem	1851:1867	the gut ecosystem	1851:1867	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	4	127	theme	PWG	801:803	arg1	interventions					805:817	the ELG and PWG interventions	789:817	the ELG and PWG interventions	789:817	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
34705551	0	128	theme	Intervention	75:86	arg1	Effects					13:19	Differential Effects	0:19	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.	0:152	Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.					
34705551	13	129	theme	important	2484:2492	arg1	concentrations					2444:2457	increasing concentrations	2433:2457	increasing concentrations	2433:2457	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	13	129	theme	important	2484:2492	arg1	metabolite					2504:2513	an important microbial metabolite	2481:2513	an important microbial metabolite involved in the colon of weaning piglets	2481:2554	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	12	130	theme	different	2158:2166	arg1	effects					2168:2174	different effects	2158:2174	different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets	2158:2278	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	12	131	from	effects	2168:2174	arg1	composition					2193:2203	the bacterial composition	2179:2203	the bacterial composition of different colonic niches	2179:2231	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	12	131	from	effects	2168:2174	arg1	function					2248:2255	colonic function	2240:2255	colonic function in the weaning piglets	2240:2278	In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets.					
34705551	6	132	from	producers	1275:1283	arg1	niches					1306:1311	different colonic niches	1288:1311	different colonic niches	1288:1311	In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta.					
34705551	8	133	theme	colonic	1581:1587	arg1	enrichment					1603:1612	colonic SCFA producer enrichment	1581:1612	colonic SCFA producer enrichment	1581:1612	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	13	134	theme	increasing	2433:2442	arg1	concentrations					2444:2457	increasing concentrations	2433:2457	increasing concentrations	2433:2457	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	13	134	theme	increasing	2433:2442	arg1	metabolite					2504:2513	an important microbial metabolite	2481:2513	an important microbial metabolite involved in the colon of weaning piglets	2481:2554	Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets.					
34705551	10	135	theme	effective	1875:1883	arg1	IMPORTANCE					1814:1823	IMPORTANCE	1814:1823	IMPORTANCE Reducing the disorders of the gut ecosystem	1814:1867	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	10	135	theme	effective	1875:1883	arg1	way					1885:1887	an effective way	1872:1887	an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry	1872:1984	IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry.					
34705551	2	136	contain	have	515:518	arg1	intervention					496:507	the combined ELG and postweaning GOS (PWG) intervention	453:507	the combined ELG and postweaning GOS (PWG) intervention	453:507	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	2	136	contain	have	515:518	arg2	effect					535:540	an interacting effect	520:540	an interacting effect	520:540	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	2	137	theme	early-life	353:362	arg1	intervention					374:385	the early-life GOS (ELG) intervention	349:385	the early-life GOS (ELG) intervention	349:385	However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored.					
34705551	1	138	theme	colonic	256:262	arg1	function					264:271	the colonic function	252:271	the colonic function	252:271	Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets.					
34705551	5	139	theme	different	998:1006	arg1	digesta					1035:1041	digesta	1035:1041	digesta	1035:1041	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	5	139	theme	different	998:1006	arg1	mucosa					1024:1029	mucosa	1024:1029	mucosa	1024:1029	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	5	139	theme	different	998:1006	arg1	niches					1016:1021	different colonic niches	998:1021	different colonic niches (mucosa and digesta)	998:1042	After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed.					
34705551	8	140	theme	producer	1594:1601	arg1	enrichment					1603:1612	colonic SCFA producer enrichment	1581:1612	colonic SCFA producer enrichment	1581:1612	Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration.					
34705551	3	141	theme	interventions	688:700	arg1	effects					661:667	the differential effects	644:667	the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets	644:762	In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets.					
34705551	4	142	theme	ELG	793:795	arg1	interventions					805:817	the ELG and PWG interventions	789:817	the ELG and PWG interventions	789:817	Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes.					
33742392	5	0	theme	cancer	763:768	arg1	context					752:758	the context	748:758	the context of cancer	748:768	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	4	1	theme	defined	695:701	arg1	stiffness					703:711	defined stiffness	695:711	defined stiffness	695:711	This model therefore allows users to examine the effect of certain treatment conditions while cells are embedded within a hydrogel of defined stiffness.					
33742392	3	2	theme	spheroid	477:484	arg1	formation					486:494	spheroid formation	477:494	spheroid formation	477:494	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	6	3	theme	spheroid	1314:1321	arg1	size					1323:1326	spheroid size	1314:1326	spheroid size	1314:1326	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	5	4	theme	extracellular	898:910	arg1	composition					919:929	the extracellular matrix composition	894:929	the extracellular matrix composition	894:929	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	5	5	theme	matrix	912:917	arg1	composition					919:929	the extracellular matrix composition	894:929	the extracellular matrix composition	894:929	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	5	6	theme	homogeneous	1014:1024	arg1	networks					1051:1058	homogeneous interpenetrating polymer networks	1014:1058	homogeneous interpenetrating polymer networks of collagen and alginate	1014:1083	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	5	7	theme	polymer	1043:1049	arg1	networks					1051:1058	homogeneous interpenetrating polymer networks	1014:1058	homogeneous interpenetrating polymer networks of collagen and alginate	1014:1083	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	1	8	theme	Three-dimensional	41:57	arg1	models					59:64	Three-dimensional models	41:64	Three-dimensional models of spheroid formation	41:86	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	6	9	theme	breast	1194:1199	arg1	lines					1187:1191	numerous malignant cell lines	1163:1191	numerous malignant cell lines (breast cancer, lung cancer, and melanoma)	1163:1234	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	9	theme	breast	1194:1199	arg1	cancer					1201:1206	breast cancer	1194:1206	breast cancer	1194:1206	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	10	theme	lung	1209:1212	arg1	lines					1187:1191	numerous malignant cell lines	1163:1191	numerous malignant cell lines (breast cancer, lung cancer, and melanoma)	1163:1234	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	10	theme	lung	1209:1212	arg1	cancer					1214:1219	lung cancer	1209:1219	lung cancer	1209:1219	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	4	11	theme	certain	620:626	arg1	conditions					638:647	certain treatment conditions	620:647	certain treatment conditions	620:647	This model therefore allows users to examine the effect of certain treatment conditions while cells are embedded within a hydrogel of defined stiffness.					
33742392	3	12	theme	three-dimensional	530:546	arg1	environment					548:558	a biomechanically tunable three-dimensional environment	504:558	a biomechanically tunable three-dimensional environment	504:558	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	6	13	theme	overall	1350:1356	arg1	viability					1363:1371	overall cell viability	1350:1371	overall cell viability	1350:1371	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	2	14	dep	in	264:265	arg1	vivo					267:270	vivo	267:270	vivo	267:270	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	3	15	theme	hydrogel	457:464	arg1	system					466:471	readily accessible composite alginate hydrogel system	419:471	readily accessible composite alginate hydrogel system	419:471	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	3	16	theme	composite	438:446	arg1	system					466:471	readily accessible composite alginate hydrogel system	419:471	readily accessible composite alginate hydrogel system	419:471	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	5	17	theme	widespread	872:881	arg1	changes					883:889	widespread changes	872:889	widespread changes in the extracellular matrix composition	872:929	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	1	18	theme	colony	145:150	arg1	capacity					160:167	the colony forming capacity	141:167	the colony forming capacity of malignant cells in an in vitro setting	141:209	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	5	19	theme	wide	795:798	arg1	range					800:804	a wide range	793:804	a wide range of mechanical properties	793:829	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	3	20	theme	adapted	370:376	arg1	protocol					378:385	This adapted protocol	365:385	This adapted protocol	365:385	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	4	21	theme	treatment	628:636	arg1	conditions					638:647	certain treatment conditions	620:647	certain treatment conditions	620:647	This model therefore allows users to examine the effect of certain treatment conditions while cells are embedded within a hydrogel of defined stiffness.					
33742392	1	22	theme	spheroid	69:76	arg1	formation					78:86	spheroid formation	69:86	spheroid formation	69:86	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	6	23	theme	spheroid	1141:1148	arg1	formation					1150:1158	single-cell spheroid formation	1129:1158	single-cell spheroid formation	1129:1158	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	1	24	theme	forming	152:158	arg1	capacity					160:167	the colony forming capacity	141:167	the colony forming capacity of malignant cells in an in vitro setting	141:209	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	6	25	theme	biomechanical	1470:1482	arg1	background					1484:1493	a controlled biomechanical background	1457:1493	a controlled biomechanical background	1457:1493	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	26	theme	single-cell	1129:1139	arg1	formation					1150:1158	single-cell spheroid formation	1129:1158	single-cell spheroid formation	1129:1158	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	1	27	theme	formation	78:86	arg1	models					59:64	Three-dimensional models	41:64	Three-dimensional models of spheroid formation	41:86	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	2	28	theme	certain	335:341	arg1	conditions					353:362	certain treatment conditions	335:362	certain treatment conditions	335:362	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	0	29	theme	In	0:1	arg1	Models					12:17	In Vitro 3D Models	0:17	In Vitro 3D Models of Tunable Stiffness	0:38	In Vitro 3D Models of Tunable Stiffness.					
33742392	2	30	theme	in	264:265	arg1	testing					272:278	in vivo testing	264:278	in vivo testing	264:278	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	5	31	from	changes	883:889	arg1	composition					919:929	the extracellular matrix composition	894:929	the extracellular matrix composition	894:929	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	5	32	theme	mechanical	809:818	arg1	properties					820:829	mechanical properties	809:829	mechanical properties	809:829	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	6	33	dep	in	1430:1431	arg1	vitro					1433:1437	vitro	1433:1437	vitro	1433:1437	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	5	34	theme	high-throughput	974:988	arg1	method					990:995	a high-throughput method	972:995	a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate	972:1083	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	1	35	theme	malignant	172:180	arg1	cells					182:186	malignant cells	172:186	malignant cells	172:186	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	0	36	theme	3D	9:10	arg1	Models					12:17	In Vitro 3D Models	0:17	In Vitro 3D Models of Tunable Stiffness	0:38	In Vitro 3D Models of Tunable Stiffness.					
33742392	6	37	theme	cell	1358:1361	arg1	viability					1363:1371	overall cell viability	1350:1371	overall cell viability	1350:1371	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	5	38	from	important	735:743	arg1	context					752:758	the context	748:758	the context of cancer	748:768	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	1	39	used	used	108:111	arg2	models					59:64	Three-dimensional models	41:64	Three-dimensional models of spheroid formation	41:86	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	1	40	theme	cells	182:186	arg1	capacity					160:167	the colony forming capacity	141:167	the colony forming capacity of malignant cells in an in vitro setting	141:209	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	0	41	theme	Tunable	22:28	arg1	Stiffness					30:38	Tunable Stiffness	22:38	Tunable Stiffness	22:38	In Vitro 3D Models of Tunable Stiffness.					
33742392	6	42	theme	controlled	1459:1468	arg1	background					1484:1493	a controlled biomechanical background	1457:1493	a controlled biomechanical background	1457:1493	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	5	43	theme	collagen	1063:1070	arg1	networks					1051:1058	homogeneous interpenetrating polymer networks	1014:1058	homogeneous interpenetrating polymer networks of collagen and alginate	1014:1083	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	2	44	theme	conditions	353:362	arg1	effect					325:330	the effect	321:330	the effect of certain treatment conditions	321:362	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	1	45	dep	in	194:195	arg1	vitro					197:201	vitro	197:201	vitro	197:201	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	4	46	theme	conditions	638:647	arg1	effect					610:615	the effect	606:615	the effect of certain treatment conditions	606:647	This model therefore allows users to examine the effect of certain treatment conditions while cells are embedded within a hydrogel of defined stiffness.					
33742392	6	47	theme	in	1430:1431	arg1	screening					1439:1447	in vitro screening	1430:1447	in vitro screening against a controlled biomechanical background	1430:1493	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	47	theme	in	1430:1431	arg1	high-throughput					1413:1427	high-throughput	1413:1427	high-throughput	1413:1427	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	48	dep	show	1089:1092	arg1	allowing					1385:1392	allowing	1385:1392	allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background	1385:1493	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	5	49	theme	properties	820:829	arg1	range					800:804	a wide range	793:804	a wide range of mechanical properties	793:829	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	3	50	theme	high	399:402	arg1	throughput					404:413	a high throughput	397:413	a high throughput	397:413	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	5	51	theme	alginate	1076:1083	arg1	networks					1051:1058	homogeneous interpenetrating polymer networks	1014:1058	homogeneous interpenetrating polymer networks of collagen and alginate	1014:1083	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	6	52	theme	cell	1182:1185	arg1	lines					1187:1191	numerous malignant cell lines	1163:1191	numerous malignant cell lines (breast cancer, lung cancer, and melanoma)	1163:1234	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	52	theme	cell	1182:1185	arg1	cancer					1201:1206	breast cancer	1194:1206	breast cancer	1194:1206	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	52	theme	cell	1182:1185	arg1	cancer					1214:1219	lung cancer	1209:1219	lung cancer	1209:1219	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	52	theme	cell	1182:1185	arg1	melanoma					1226:1233	melanoma	1226:1233	melanoma	1226:1233	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	2	53	theme	large-scale	290:300	arg1	interrogation					302:314	large-scale interrogation	290:314	large-scale interrogation	290:314	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	0	54	theme	Stiffness	30:38	arg1	Models					12:17	In Vitro 3D Models	0:17	In Vitro 3D Models of Tunable Stiffness	0:38	In Vitro 3D Models of Tunable Stiffness.					
33742392	6	55	theme	formation	1287:1295	arg1	number					1338:1343	spheroid number	1329:1343	spheroid number	1329:1343	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	55	theme	formation	1287:1295	arg1	measures					1297:1304	colony formation measures	1280:1304	colony formation measures such as spheroid size, spheroid number, and overall cell viability	1280:1371	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	55	theme	formation	1287:1295	arg1	size					1323:1326	spheroid size	1314:1326	spheroid size	1314:1326	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	55	theme	formation	1287:1295	arg1	viability					1363:1371	overall cell viability	1350:1371	overall cell viability	1350:1371	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	4	56	theme	stiffness	703:711	arg1	hydrogel					683:690	a hydrogel	681:690	a hydrogel of defined stiffness	681:711	This model therefore allows users to examine the effect of certain treatment conditions while cells are embedded within a hydrogel of defined stiffness.					
33742392	6	57	dep	lines	1187:1191	arg1	lines					1187:1191	numerous malignant cell lines	1163:1191	numerous malignant cell lines (breast cancer, lung cancer, and melanoma)	1163:1234	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	57	dep	lines	1187:1191	arg1	cancer					1201:1206	breast cancer	1194:1206	breast cancer	1194:1206	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	57	dep	lines	1187:1191	arg1	cancer					1214:1219	lung cancer	1209:1219	lung cancer	1209:1219	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	57	dep	lines	1187:1191	arg1	melanoma					1226:1233	melanoma	1226:1233	melanoma	1226:1233	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	58	theme	spheroid	1329:1336	arg1	number					1338:1343	spheroid number	1329:1343	spheroid number	1329:1343	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	5	59	theme	interpenetrating	1026:1041	arg1	networks					1051:1058	homogeneous interpenetrating polymer networks	1014:1058	homogeneous interpenetrating polymer networks of collagen and alginate	1014:1083	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	6	60	theme	colony	1280:1285	arg1	formation					1287:1295	colony formation	1280:1295	colony formation measures such as spheroid size, spheroid number, and overall cell viability	1280:1371	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	3	61	theme	alginate	448:455	arg1	system					466:471	readily accessible composite alginate hydrogel system	419:471	readily accessible composite alginate hydrogel system	419:471	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	6	62	theme	malignant	1172:1180	arg1	lines					1187:1191	numerous malignant cell lines	1163:1191	numerous malignant cell lines (breast cancer, lung cancer, and melanoma)	1163:1234	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	62	theme	malignant	1172:1180	arg1	cancer					1201:1206	breast cancer	1194:1206	breast cancer	1194:1206	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	62	theme	malignant	1172:1180	arg1	cancer					1214:1219	lung cancer	1209:1219	lung cancer	1209:1219	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	62	theme	malignant	1172:1180	arg1	melanoma					1226:1233	melanoma	1226:1233	melanoma	1226:1233	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	1	63	theme	in	194:195	arg1	setting					203:209	an in vitro setting	191:209	an in vitro setting	191:209	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	2	64	theme	model	226:230	arg1	Use					212:214	Use	212:214	Use of such a model	212:230	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	5	65	from	context	752:758	arg1	important					735:743	important	735:743	important	735:743	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	2	66	theme	robust	243:248	arg1	surrogate					250:258	a robust surrogate	241:258	a robust surrogate for in vivo testing	241:278	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	1	67	from	capacity	160:167	arg1	setting					203:209	an in vitro setting	191:209	an in vitro setting	191:209	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	1	68	theme	cancer	120:125	arg1	field					127:131	the cancer field	116:131	the cancer field	116:131	Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting.					
33742392	5	69	theme	architecture.This	935:951	arg1	protocol					953:960	architecture.This protocol	935:960	architecture.This protocol	935:960	This is particularly important in the context of cancer where cells experience a wide range of mechanical properties within their microenvironment, driven by widespread changes in the extracellular matrix composition and architecture.This protocol describes a high-throughput method which results in homogeneous interpenetrating polymer networks of collagen and alginate.					
33742392	3	70	theme	accessible	427:436	arg1	system					466:471	readily accessible composite alginate hydrogel system	419:471	readily accessible composite alginate hydrogel system	419:471	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	6	71	theme	numerous	1163:1170	arg1	lines					1187:1191	numerous malignant cell lines	1163:1191	numerous malignant cell lines (breast cancer, lung cancer, and melanoma)	1163:1234	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	71	theme	numerous	1163:1170	arg1	cancer					1201:1206	breast cancer	1194:1206	breast cancer	1194:1206	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	71	theme	numerous	1163:1170	arg1	cancer					1214:1219	lung cancer	1209:1219	lung cancer	1209:1219	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	6	71	theme	numerous	1163:1170	arg1	melanoma					1226:1233	melanoma	1226:1233	melanoma	1226:1233	We show that this network readily supports single-cell spheroid formation in numerous malignant cell lines (breast cancer, lung cancer, and melanoma) and that these can be robustly analyzed for colony formation measures such as spheroid size, spheroid number, and overall cell viability; therefore, allowing users to undertake high-throughput, in vitro screening against a controlled biomechanical background.					
33742392	2	72	theme	treatment	343:351	arg1	conditions					353:362	certain treatment conditions	335:362	certain treatment conditions	335:362	Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions.					
33742392	3	73	theme	tunable	522:528	arg1	environment					548:558	a biomechanically tunable three-dimensional environment	504:558	a biomechanically tunable three-dimensional environment	504:558	This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid formation, within a biomechanically tunable three-dimensional environment.					
33742392	0	74	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro 3D Models of Tunable Stiffness.					
34842126	9	0	theme	sugar	929:933	arg1	%					952:952	7·6 E %	946:952	7·6 E % in women	946:961	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	0	theme	sugar	929:933	arg1	intake					935:940	The average added sugar intake	911:940	The average added sugar intake	911:940	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	10	1	theme	<	1121:1121	arg1	activity					1109:1116	physical activity	1100:1116	physical activity (P < 0·0001)	1100:1129	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	10	1	theme	<	1121:1121	arg1	0·0001					1123:1128	P < 0·0001	1119:1128	P < 0·0001	1119:1128	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	12	2	dep	stronger	1422:1429	arg1	=					1505:1505	=	1505:1505	=	1505:1505	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	10	3	dep	=	1070:1070	arg1	women					1077:1081	0·03 women	1072:1081	0·03 women	1072:1081	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	10	4	from	BMI	1136:1138	arg1	men					1143:1145	men	1143:1145	men	1143:1145	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	9	5	theme	E	950:950	arg1	%					952:952	7·6 E %	946:952	7·6 E % in women	946:961	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	5	theme	E	950:950	arg1	intake					935:940	The average added sugar intake	911:940	The average added sugar intake	911:940	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	5	6	theme	validated	602:610	arg1	FFQ					612:614	a validated FFQ	600:614	a validated FFQ	600:614	Dietary intake was assessed by a validated FFQ.					
34842126	13	7	theme	lifestyle	1614:1622	arg1	habits					1624:1629	several unhealthy dietary and lifestyle habits	1584:1629	habits	1624:1629	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	13	8	theme	added	1546:1550	arg1	intake					1558:1563	high added sugar intake	1541:1563	high added sugar intake	1541:1563	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	2	9	theme	sugar	289:293	arg1	intake					295:300	added sugar intake	283:300	added sugar intake	283:300	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	10	10	dep	P	1187:1187	arg1	men					1215:1217	P < 0·0001 men	1204:1217	P < 0·0001 men	1204:1217	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	1	11	theme	few	168:170	arg1	studies					172:178	few studies	168:178	few studies	168:178	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	12	12	theme	inverse	1396:1402	arg1	association					1404:1414	the inverse association	1392:1414	the inverse association	1392:1414	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	12	12	theme	inverse	1396:1402	arg1	stronger					1422:1429	stronger	1422:1429	stronger	1422:1429	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	7	13	theme	sugar	823:827	arg1	sources					829:835	identifying naturally occurring sugar sources	791:835	identifying naturally occurring sugar sources	791:835	Added sugar intake was estimated based on food categorisation and identifying naturally occurring sugar sources.					
34842126	8	14	theme	Diet	899:902	arg1	Score					904:908	the modified Baltic Sea Diet Score	875:908	the modified Baltic Sea Diet Score	875:908	Overall diet quality was assessed by the modified Baltic Sea Diet Score.					
34842126	9	15	theme	E	971:971	arg1	%					973:973	8·3 E %	967:973	8·3 E % in men	967:980	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	7	16	theme	Added	725:729	arg1	intake					737:742	Added sugar intake	725:742	Added sugar intake	725:742	Added sugar intake was estimated based on food categorisation and identifying naturally occurring sugar sources.					
34842126	0	17	from	quality	56:62	arg1	population					85:94	the Finnish adult population	67:94	the Finnish adult population	67:94	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	10	18	theme	sugar	1014:1018	arg1	intake					1020:1025	Added sugar intake	1008:1025	Added sugar intake	1008:1025	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	11	19	theme	<	1308:1308	arg1	sexes					1299:1303	both sexes	1294:1303	both sexes (P < 0·0001)	1294:1316	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	11	19	theme	<	1308:1308	arg1	0·0001					1310:1315	P < 0·0001	1306:1315	P < 0·0001	1306:1315	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	14	20	dep	consider	1756:1763	arg1	added					1765:1769	added	1765:1769	should consider added sugar intake equally in the whole population	1749:1814	Efforts to improve diet quality should consider added sugar intake equally in the whole population.					
34842126	8	21	theme	Overall	838:844	arg1	quality					851:857	Overall diet quality	838:857	Overall diet quality	838:857	Overall diet quality was assessed by the modified Baltic Sea Diet Score.					
34842126	6	22	theme	Food	617:620	arg1	consumption					622:632	Food consumption	617:632	Food consumption	617:632	Food consumption and nutrient intakes were calculated using the Finnish national food composition database.					
34842126	1	23	theme	health	149:154	arg1	issues					156:161	several health issues	141:161	several health issues	141:161	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	2	24	from	intake	295:300	arg1	adults					338:343	Finnish adults	330:343	Finnish adults	330:343	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	13	25	theme	Finnish	1701:1707	arg1	adults					1709:1714	Finnish adults	1701:1714	Finnish adults	1701:1714	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	0	26	theme	adult	79:83	arg1	population					85:94	the Finnish adult population	67:94	the Finnish adult population	67:94	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	12	27	theme	active	1464:1469	arg1	men					1483:1485	moderately active or inactive men	1453:1485	moderately active or inactive men	1453:1485	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	6	28	theme	national	689:696	arg1	database					715:722	the Finnish national food composition database	677:722	the Finnish national food composition database	677:722	Food consumption and nutrient intakes were calculated using the Finnish national food composition database.					
34842126	13	29	from	inactivity	1687:1696	arg1	adults					1709:1714	Finnish adults	1701:1714	Finnish adults	1701:1714	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	12	30	theme	inactive	1474:1481	arg1	men					1483:1485	moderately active or inactive men	1453:1485	moderately active or inactive men	1453:1485	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	11	31	theme	overall	1270:1276	arg1	quality					1283:1289	overall diet quality	1270:1289	overall diet quality in both sexes (P < 0·0001)	1270:1316	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	3	32	theme	added	367:371	arg1	intake					379:384	added sugar intake	367:384	added sugar intake	367:384	Associations between added sugar intake and sociodemographic factors, lifestyle factors, and BMI were also explored.					
34842126	0	33	from	intake	32:37	arg1	population					85:94	the Finnish adult population	67:94	the Finnish adult population	67:94	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	10	34	theme	=	1150:1150	arg1	BMI					1136:1138	BMI	1136:1138	BMI in men (P = 0·003)	1136:1157	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	10	34	theme	=	1150:1150	arg1	P					1148:1148	P = 0·003	1148:1156	P = 0·003	1148:1156	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	12	35	theme	active	1437:1442	arg1	men					1444:1446	active men	1437:1446	active men	1437:1446	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	0	36	theme	added	20:24	arg1	intake					32:37	added sugar intake	20:37	added sugar intake	20:37	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	1	37	theme	Added	97:101	arg1	intake					109:114	Added sugar intake	97:114	Added sugar intake	97:114	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	3	38	theme	sociodemographic	390:405	arg1	factors					407:413	sociodemographic factors	390:413	sociodemographic factors	390:413	Associations between added sugar intake and sociodemographic factors, lifestyle factors, and BMI were also explored.					
34842126	4	39	theme	National	538:545	arg1	Study					562:566	the National FinHealth 2017 Study	534:566	the National FinHealth 2017 Study	534:566	Our data comprised 5094 adults residing in Finland who participated in the National FinHealth 2017 Study.					
34842126	9	40	theme	study	990:994	arg1	population					996:1005	this study population	985:1005	this study population	985:1005	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	7	41	theme	food	767:770	arg1	categorisation					772:785	food categorisation	767:785	food categorisation	767:785	Added sugar intake was estimated based on food categorisation and identifying naturally occurring sugar sources.					
34842126	11	42	from	quality	1283:1289	arg1	sexes					1299:1303	both sexes	1294:1303	both sexes (P < 0·0001)	1294:1316	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	11	42	from	quality	1283:1289	arg1	0·0001					1310:1315	P < 0·0001	1306:1315	P < 0·0001	1306:1315	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	11	43	theme	sugar	1227:1231	arg1	intake					1233:1238	Added sugar intake	1221:1238	Added sugar intake	1221:1238	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	7	44	theme	identifying	791:801	arg1	sources					829:835	identifying naturally occurring sugar sources	791:835	identifying naturally occurring sugar sources	791:835	Added sugar intake was estimated based on food categorisation and identifying naturally occurring sugar sources.					
34842126	0	45	theme	overall	43:49	arg1	quality					56:62	overall diet quality	43:62	overall diet quality	43:62	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	14	46	theme	sugar	1771:1775	arg1	intake					1777:1782	sugar intake	1771:1782	sugar intake	1771:1782	Efforts to improve diet quality should consider added sugar intake equally in the whole population.					
34842126	2	47	theme	overall	306:312	arg1	quality					319:325	overall diet quality	306:325	overall diet quality	306:325	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	10	48	theme	P	1204:1204	arg1	men					1215:1217	P < 0·0001 men	1204:1217	P < 0·0001 men	1204:1217	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	7	49	theme	sugar	731:735	arg1	intake					737:742	Added sugar intake	725:742	Added sugar intake	725:742	Added sugar intake was estimated based on food categorisation and identifying naturally occurring sugar sources.					
34842126	10	50	dep	=	1086:1086	arg1	P					1068:1068	P	1068:1068	P = 0·03 women	1068:1081	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	1	51	with	association	198:208	arg1	quality					228:234	overall diet quality	215:234	overall diet quality	215:234	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	9	52	theme	added	923:927	arg1	%					952:952	7·6 E %	946:952	7·6 E % in women	946:961	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	52	theme	added	923:927	arg1	intake					935:940	The average added sugar intake	911:940	The average added sugar intake	911:940	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	6	53	theme	composition	703:713	arg1	database					715:722	the Finnish national food composition database	677:722	the Finnish national food composition database	677:722	Food consumption and nutrient intakes were calculated using the Finnish national food composition database.					
34842126	2	54	theme	Finnish	330:336	arg1	adults					338:343	Finnish adults	330:343	Finnish adults	330:343	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	4	55	theme	2017	557:560	arg1	Study					562:566	the National FinHealth 2017 Study	534:566	the National FinHealth 2017 Study	534:566	Our data comprised 5094 adults residing in Finland who participated in the National FinHealth 2017 Study.					
34842126	10	56	theme	P	1119:1119	arg1	activity					1109:1116	physical activity	1100:1116	physical activity (P < 0·0001)	1100:1129	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	10	56	theme	P	1119:1119	arg1	0·0001					1123:1128	P < 0·0001	1119:1128	P < 0·0001	1119:1128	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	2	57	from	quality	319:325	arg1	adults					338:343	Finnish adults	330:343	Finnish adults	330:343	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	11	58	theme	Added	1221:1225	arg1	intake					1233:1238	Added sugar intake	1221:1238	Added sugar intake	1221:1238	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	13	59	theme	high	1541:1544	arg1	intake					1558:1563	high added sugar intake	1541:1563	high added sugar intake	1541:1563	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	8	60	theme	Baltic	888:893	arg1	Score					904:908	the modified Baltic Sea Diet Score	875:908	the modified Baltic Sea Diet Score	875:908	Overall diet quality was assessed by the modified Baltic Sea Diet Score.					
34842126	10	61	dep	education	1057:1065	arg1	=					1086:1086	=	1086:1086	=	1086:1086	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	13	62	theme	sugar	1552:1556	arg1	intake					1558:1563	high added sugar intake	1541:1563	high added sugar intake	1541:1563	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	2	63	theme	added	283:287	arg1	intake					295:300	added sugar intake	283:300	added sugar intake	283:300	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	1	64	theme	sugar	103:107	arg1	intake					109:114	Added sugar intake	97:114	Added sugar intake	97:114	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	11	65	theme	P	1306:1306	arg1	sexes					1299:1303	both sexes	1294:1303	both sexes (P < 0·0001)	1294:1316	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	11	65	theme	P	1306:1306	arg1	0·0001					1310:1315	P < 0·0001	1306:1315	P < 0·0001	1306:1315	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	10	66	theme	Added	1008:1012	arg1	intake					1020:1025	Added sugar intake	1008:1025	Added sugar intake	1008:1025	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	12	67	theme	men	1357:1359	arg1	subgroups					1381:1389	men's physical activity subgroups	1357:1389	men's physical activity subgroups	1357:1389	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	14	68	dep	intake	1777:1782	arg1	population					1805:1814	the whole population	1795:1814	the whole population	1795:1814	Efforts to improve diet quality should consider added sugar intake equally in the whole population.					
34842126	12	69	theme	physical	1363:1370	arg1	activity					1372:1379	physical activity	1363:1379	men's physical activity subgroups	1357:1389	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	9	70	from	%	973:973	arg1	women					957:961	women	957:961	women	957:961	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	70	from	%	973:973	arg1	men					978:980	men	978:980	men	978:980	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	70	from	%	973:973	arg1	population					996:1005	this study population	985:1005	this study population	985:1005	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	13	71	theme	leisure-time	1674:1685	arg1	inactivity					1687:1696	leisure-time inactivity	1674:1696	leisure-time inactivity in Finnish adults	1674:1714	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	13	71	theme	leisure-time	1674:1685	arg1	diets					1655:1659	poor-quality diets	1642:1659	poor-quality diets	1642:1659	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	0	72	theme	Finnish	71:77	arg1	population					85:94	the Finnish adult population	67:94	the Finnish adult population	67:94	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	1	73	theme	several	141:147	arg1	issues					156:161	several health issues	141:161	several health issues	141:161	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	8	74	theme	diet	846:849	arg1	quality					851:857	Overall diet quality	838:857	Overall diet quality	838:857	Overall diet quality was assessed by the modified Baltic Sea Diet Score.					
34842126	12	75	theme	Pfor	1488:1491	arg1	interaction					1493:1503	Pfor interaction	1488:1503	Pfor interaction	1488:1503	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	6	76	theme	food	698:701	arg1	database					715:722	the Finnish national food composition database	677:722	the Finnish national food composition database	677:722	Food consumption and nutrient intakes were calculated using the Finnish national food composition database.					
34842126	9	77	from	%	952:952	arg1	women					957:961	women	957:961	women	957:961	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	77	from	%	952:952	arg1	men					978:980	men	978:980	men	978:980	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	77	from	%	952:952	arg1	population					996:1005	this study population	985:1005	this study population	985:1005	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	13	78	theme	poor-quality	1642:1653	arg1	smoking					1662:1668	smoking	1662:1668	smoking	1662:1668	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	13	78	theme	poor-quality	1642:1653	arg1	inactivity					1687:1696	leisure-time inactivity	1674:1696	leisure-time inactivity in Finnish adults	1674:1714	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	13	78	theme	poor-quality	1642:1653	arg1	diets					1655:1659	poor-quality diets	1642:1659	poor-quality diets	1642:1659	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	6	79	theme	Finnish	681:687	arg1	database					715:722	the Finnish national food composition database	677:722	the Finnish national food composition database	677:722	Food consumption and nutrient intakes were calculated using the Finnish national food composition database.					
34842126	6	80	theme	nutrient	638:645	arg1	intakes					647:653	nutrient intakes	638:653	nutrient intakes	638:653	Food consumption and nutrient intakes were calculated using the Finnish national food composition database.					
34842126	14	81	theme	whole	1799:1803	arg1	population					1805:1814	the whole population	1795:1814	the whole population	1795:1814	Efforts to improve diet quality should consider added sugar intake equally in the whole population.					
34842126	8	82	theme	modified	879:886	arg1	Score					904:908	the modified Baltic Sea Diet Score	875:908	the modified Baltic Sea Diet Score	875:908	Overall diet quality was assessed by the modified Baltic Sea Diet Score.					
34842126	3	83	theme	sugar	373:377	arg1	intake					379:384	added sugar intake	367:384	added sugar intake	367:384	Associations between added sugar intake and sociodemographic factors, lifestyle factors, and BMI were also explored.					
34842126	3	84	theme	lifestyle	416:424	arg1	factors					426:432	lifestyle factors	416:432	lifestyle factors	416:432	Associations between added sugar intake and sociodemographic factors, lifestyle factors, and BMI were also explored.					
34842126	0	85	theme	sugar	26:30	arg1	intake					32:37	added sugar intake	20:37	added sugar intake	20:37	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	7	86	theme	occurring	813:821	arg1	sources					829:835	identifying naturally occurring sugar sources	791:835	identifying naturally occurring sugar sources	791:835	Added sugar intake was estimated based on food categorisation and identifying naturally occurring sugar sources.					
34842126	5	87	theme	Dietary	569:575	arg1	intake					577:582	Dietary intake	569:582	Dietary intake	569:582	Dietary intake was assessed by a validated FFQ.					
34842126	8	88	theme	Sea	895:897	arg1	Score					904:908	the modified Baltic Sea Diet Score	875:908	the modified Baltic Sea Diet Score	875:908	Overall diet quality was assessed by the modified Baltic Sea Diet Score.					
34842126	4	89	theme	FinHealth	547:555	arg1	Study					562:566	the National FinHealth 2017 Study	534:566	the National FinHealth 2017 Study	534:566	Our data comprised 5094 adults residing in Finland who participated in the National FinHealth 2017 Study.					
34842126	11	90	theme	diet	1278:1281	arg1	quality					1283:1289	overall diet quality	1270:1289	overall diet quality in both sexes (P < 0·0001)	1270:1316	Added sugar intake was inversely associated with overall diet quality in both sexes (P < 0·0001).					
34842126	10	91	theme	=	1070:1070	arg1	P					1068:1068	P	1068:1068	P = 0·03 women	1068:1081	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	1	92	theme	overall	215:221	arg1	quality					228:234	overall diet quality	215:234	overall diet quality	215:234	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
34842126	0	93	theme	diet	51:54	arg1	quality					56:62	overall diet quality	43:62	overall diet quality	43:62	Association between added sugar intake and overall diet quality in the Finnish adult population.					
34842126	2	94	theme	diet	314:317	arg1	quality					319:325	overall diet quality	306:325	overall diet quality	306:325	We aimed at examining the association between added sugar intake and overall diet quality in Finnish adults.					
34842126	13	95	from	smoking	1662:1668	arg1	adults					1709:1714	Finnish adults	1701:1714	Finnish adults	1701:1714	Our findings suggest that high added sugar intake is associated with several unhealthy dietary and lifestyle habits, including poor-quality diets, smoking and leisure-time inactivity in Finnish adults.					
34842126	12	96	dep	men	1357:1359	arg1	activity					1372:1379	physical activity	1363:1379	men's physical activity subgroups	1357:1389	No interactions were found except for men's physical activity subgroups, the inverse association being stronger among active men than moderately active or inactive men (Pfor interaction = 0·005).					
34842126	10	97	theme	<	1206:1206	arg1	men					1215:1217	P < 0·0001 men	1204:1217	P < 0·0001 men	1204:1217	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	10	98	theme	physical	1100:1107	arg1	activity					1109:1116	physical activity	1100:1116	physical activity (P < 0·0001)	1100:1129	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	10	98	theme	physical	1100:1107	arg1	0·0001					1123:1128	P < 0·0001	1119:1128	P < 0·0001	1119:1128	Added sugar intake was inversely associated with education (P = 0·03 women; P = 0·001 men), physical activity (P < 0·0001), and BMI in men (P = 0·003), and directly with smoking (P = 0·002 women; P < 0·0001 men).					
34842126	14	99	theme	diet	1736:1739	arg1	quality					1741:1747	diet quality	1736:1747	diet quality	1736:1747	Efforts to improve diet quality should consider added sugar intake equally in the whole population.					
34842126	9	100	theme	average	915:921	arg1	%					952:952	7·6 E %	946:952	7·6 E % in women	946:961	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	9	100	theme	average	915:921	arg1	intake					935:940	The average added sugar intake	911:940	The average added sugar intake	911:940	The average added sugar intake was 7·6 E % in women and 8·3 E % in men in this study population.					
34842126	1	101	theme	diet	223:226	arg1	quality					228:234	overall diet quality	215:234	overall diet quality	215:234	Added sugar intake has been associated with several health issues, but few studies have examined its association with overall diet quality.					
33357862	2	0	theme	attractive	402:411	arg1	fibers					388:393	cellulose-lignin carbon fibers	364:393	cellulose-lignin carbon fibers	364:393	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	2	0	theme	attractive	402:411	arg1	material					426:433	an attractive light-weight material	399:433	an attractive light-weight material for, e.g. wind power and automobile manufacturing	399:483	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	0	1	theme	precursor	95:103	arg1	distributions					36:48	Disassociated molecular orientation distributions	0:48	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.	0:173	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	1	2	theme	Cellulose-lignin	175:190	arg1	fibers					209:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	1	2	theme	Cellulose-lignin	175:190	arg1	alternative					268:278	a potential environmentally benign alternative	233:278	a potential environmentally benign alternative to the traditional polyacrylonitrile precursor	233:325	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	3	3	theme	molecular	602:610	arg1	distribution					624:635	the molecular orientation distribution	598:635	the molecular orientation distribution of the precursor	598:652	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	1	4	theme	traditional	287:297	arg1	precursor					317:325	the traditional polyacrylonitrile precursor	283:325	the traditional polyacrylonitrile precursor	283:325	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	4	5	theme	X-ray	847:851	arg1	scattering					853:862	X-ray scattering	847:862	X-ray scattering	847:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	4	6	theme	molecular	683:691	arg1	orientation					693:703	the molecular orientation	679:703	the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	679:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	7	7	theme	native	1236:1241	arg1	wood					1243:1246	the native wood	1232:1246	the native wood	1232:1246	The current processes for fabricating a cellulose-lignin composite fiber cannot regain the oriented lignin as observed from the native wood.					
33357862	4	8	theme	solid-state	791:801	arg1	resonance					820:828	rotor-synchronized solid-state nuclear magnetic resonance	772:828	rotor-synchronized solid-state nuclear magnetic resonance spectroscopy	772:841	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	1	9	theme	polyacrylonitrile	299:315	arg1	precursor					317:325	the traditional polyacrylonitrile precursor	283:325	the traditional polyacrylonitrile precursor	283:325	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	0	10	theme	rotor	117:121	arg1	spectroscopy					140:151	rotor synchronized NMR spectroscopy	117:151	rotor synchronized NMR spectroscopy	117:151	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	5	11	theme	cellulose-lignin	948:963	arg1	fiber					975:979	a mechanically stretched cellulose-lignin composite fiber	923:979	a mechanically stretched cellulose-lignin composite fiber	923:979	Our results show that lignin is completely disordered, in a mechanically stretched cellulose-lignin composite fiber, while the cellulose is ordered.					
33357862	3	12	theme	creep	533:537	arg1	resistance					539:548	creep resistance	533:548	creep resistance	533:548	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	1	13	theme	composite	192:200	arg1	fibers					209:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	1	13	theme	composite	192:200	arg1	alternative					268:278	a potential environmentally benign alternative	233:278	a potential environmentally benign alternative to the traditional polyacrylonitrile precursor	233:325	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	7	14	theme	oriented	1199:1206	arg1	lignin					1208:1213	the oriented lignin	1195:1213	the oriented lignin	1195:1213	The current processes for fabricating a cellulose-lignin composite fiber cannot regain the oriented lignin as observed from the native wood.					
33357862	3	15	theme	carbon	579:584	arg1	content					586:592	the carbon content	575:592	the carbon content	575:592	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	1	16	theme	carbon	202:207	arg1	fibers					209:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	1	16	theme	carbon	202:207	arg1	alternative					268:278	a potential environmentally benign alternative	233:278	a potential environmentally benign alternative to the traditional polyacrylonitrile precursor	233:325	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	0	17	theme	molecular	14:22	arg1	distributions					36:48	Disassociated molecular orientation distributions	0:48	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.	0:173	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	4	18	theme	components	718:727	arg1	orientation					693:703	the molecular orientation	679:703	the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	679:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	3	19	theme	orientation	612:622	arg1	distribution					624:635	the molecular orientation distribution	598:635	the molecular orientation distribution of the precursor	598:652	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	4	20	from	components	718:727	arg1	fibers					759:764	cellulose-lignin composite fibers	732:764	cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	732:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	4	21	theme	composite	749:757	arg1	fibers					759:764	cellulose-lignin composite fibers	732:764	cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	732:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	0	22	theme	Disassociated	0:12	arg1	distributions					36:48	Disassociated molecular orientation distributions	0:48	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.	0:173	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	4	23	theme	resonance	820:828	arg1	spectroscopy					830:841	rotor-synchronized solid-state nuclear magnetic resonance spectroscopy	772:841	rotor-synchronized solid-state nuclear magnetic resonance spectroscopy	772:841	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	4	24	theme	cellulose-lignin	732:747	arg1	fibers					759:764	cellulose-lignin composite fibers	732:764	cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	732:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	6	25	theme	oriented	1077:1084	arg1	lignin					1086:1091	lignin	1086:1091	lignin	1086:1091	In contrast, the native spruce wood raw material displays both oriented lignin and cellulose.					
33357862	0	26	theme	NMR	136:138	arg1	spectroscopy					140:151	rotor synchronized NMR spectroscopy	117:151	rotor synchronized NMR spectroscopy	117:151	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	2	27	theme	cost	348:351	arg1	reduction					353:361	the associated cost reduction	333:361	the associated cost reduction	333:361	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	3	28	theme	tensile	513:519	arg1	modulus					521:527	tensile modulus	513:527	tensile modulus	513:527	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	0	29	theme	orientation	24:34	arg1	distributions					36:48	Disassociated molecular orientation distributions	0:48	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.	0:173	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	0	30	theme	synchronized	123:134	arg1	spectroscopy					140:151	rotor synchronized NMR spectroscopy	117:151	rotor synchronized NMR spectroscopy	117:151	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	2	31	theme	associated	337:346	arg1	reduction					353:361	the associated cost reduction	333:361	the associated cost reduction	333:361	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	4	32	from	orientation	693:703	arg1	fibers					759:764	cellulose-lignin composite fibers	732:764	cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	732:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	2	33	theme	automobile	460:469	arg1	manufacturing					471:483	automobile manufacturing	460:483	automobile manufacturing	460:483	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	5	34	theme	composite	965:973	arg1	fiber					975:979	a mechanically stretched cellulose-lignin composite fiber	923:979	a mechanically stretched cellulose-lignin composite fiber	923:979	Our results show that lignin is completely disordered, in a mechanically stretched cellulose-lignin composite fiber, while the cellulose is ordered.					
33357862	3	35	theme	precursor	644:652	arg1	content					586:592	the carbon content	575:592	the carbon content	575:592	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	3	35	theme	precursor	644:652	arg1	distribution					624:635	the molecular orientation distribution	598:635	the molecular orientation distribution of the precursor	598:652	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	4	36	theme	different	708:716	arg1	components					718:727	different components	708:727	different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	708:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	2	37	theme	wind	445:448	arg1	power					450:454	wind power	445:454	wind power	445:454	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	1	38	dep	potential	235:243	arg1	benign					261:266	benign	261:266	benign	261:266	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	2	39	dep	power	450:454	arg1	e.g.					440:443	e.g.	440:443	e.g.	440:443	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	0	40	theme	cellulose-lignin	65:80	arg1	precursor					95:103	a composite cellulose-lignin carbon fiber precursor	53:103	a composite cellulose-lignin carbon fiber precursor	53:103	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	6	41	theme	raw	1050:1052	arg1	material					1054:1061	the native spruce wood raw material	1027:1061	the native spruce wood raw material	1027:1061	In contrast, the native spruce wood raw material displays both oriented lignin and cellulose.					
33357862	7	42	theme	composite	1165:1173	arg1	fiber					1175:1179	a cellulose-lignin composite fiber	1146:1179	a cellulose-lignin composite fiber	1146:1179	The current processes for fabricating a cellulose-lignin composite fiber cannot regain the oriented lignin as observed from the native wood.					
33357862	3	43	theme	carbon	490:495	arg1	tenacity					503:510	The carbon fiber tenacity	486:510	The carbon fiber tenacity	486:510	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	4	44	theme	magnetic	811:818	arg1	resonance					820:828	rotor-synchronized solid-state nuclear magnetic resonance	772:828	rotor-synchronized solid-state nuclear magnetic resonance spectroscopy	772:841	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	0	45	theme	composite	55:63	arg1	precursor					95:103	a composite cellulose-lignin carbon fiber precursor	53:103	a composite cellulose-lignin carbon fiber precursor	53:103	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	6	46	theme	wood	1045:1048	arg1	material					1054:1061	the native spruce wood raw material	1027:1061	the native spruce wood raw material	1027:1061	In contrast, the native spruce wood raw material displays both oriented lignin and cellulose.					
33357862	0	47	theme	X-ray	157:161	arg1	scattering					163:172	X-ray scattering	157:172	X-ray scattering	157:172	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	7	48	theme	current	1112:1118	arg1	processes					1120:1128	The current processes	1108:1128	The current processes for fabricating a cellulose-lignin composite fiber	1108:1179	The current processes for fabricating a cellulose-lignin composite fiber cannot regain the oriented lignin as observed from the native wood.					
33357862	4	49	theme	nuclear	803:809	arg1	resonance					820:828	rotor-synchronized solid-state nuclear magnetic resonance	772:828	rotor-synchronized solid-state nuclear magnetic resonance spectroscopy	772:841	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	6	50	theme	spruce	1038:1043	arg1	material					1054:1061	the native spruce wood raw material	1027:1061	the native spruce wood raw material	1027:1061	In contrast, the native spruce wood raw material displays both oriented lignin and cellulose.					
33357862	0	51	dep	distributions	36:48	arg1	study					108:112	A study	106:112	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.	0:173	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	2	52	theme	carbon	381:386	arg1	fibers					388:393	cellulose-lignin carbon fibers	364:393	cellulose-lignin carbon fibers	364:393	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	2	52	theme	carbon	381:386	arg1	material					426:433	an attractive light-weight material	399:433	an attractive light-weight material for, e.g. wind power and automobile manufacturing	399:483	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	4	53	theme	rotor-synchronized	772:789	arg1	resonance					820:828	rotor-synchronized solid-state nuclear magnetic resonance	772:828	rotor-synchronized solid-state nuclear magnetic resonance spectroscopy	772:841	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	1	54	theme	potential	235:243	arg1	fibers					209:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers	175:214	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	1	54	theme	potential	235:243	arg1	alternative					268:278	a potential environmentally benign alternative	233:278	a potential environmentally benign alternative to the traditional polyacrylonitrile precursor	233:325	Cellulose-lignin composite carbon fibers have shown to be a potential environmentally benign alternative to the traditional polyacrylonitrile precursor.					
33357862	7	55	theme	cellulose-lignin	1148:1163	arg1	fiber					1175:1179	a cellulose-lignin composite fiber	1146:1179	a cellulose-lignin composite fiber	1146:1179	The current processes for fabricating a cellulose-lignin composite fiber cannot regain the oriented lignin as observed from the native wood.					
33357862	2	56	with	material	426:433	arg1	reduction					353:361	the associated cost reduction	333:361	the associated cost reduction	333:361	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	2	57	theme	cellulose-lignin	364:379	arg1	fibers					388:393	cellulose-lignin carbon fibers	364:393	cellulose-lignin carbon fibers	364:393	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	2	57	theme	cellulose-lignin	364:379	arg1	material					426:433	an attractive light-weight material	399:433	an attractive light-weight material for, e.g. wind power and automobile manufacturing	399:483	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	0	58	theme	fiber	89:93	arg1	precursor					95:103	a composite cellulose-lignin carbon fiber precursor	53:103	a composite cellulose-lignin carbon fiber precursor	53:103	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	4	59	from	fibers	759:764	arg1	orientation					693:703	the molecular orientation	679:703	the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering	679:862	This work disassociates the molecular orientation of different components in cellulose-lignin composite fibers using rotor-synchronized solid-state nuclear magnetic resonance spectroscopy and X-ray scattering.					
33357862	5	60	theme	stretched	938:946	arg1	fiber					975:979	a mechanically stretched cellulose-lignin composite fiber	923:979	a mechanically stretched cellulose-lignin composite fiber	923:979	Our results show that lignin is completely disordered, in a mechanically stretched cellulose-lignin composite fiber, while the cellulose is ordered.					
33357862	3	61	theme	fiber	497:501	arg1	tenacity					503:510	The carbon fiber tenacity	486:510	The carbon fiber tenacity	486:510	The carbon fiber tenacity, tensile modulus and creep resistance is in part determined by the carbon content and the molecular orientation distribution of the precursor.					
33357862	0	62	theme	carbon	82:87	arg1	precursor					95:103	a composite cellulose-lignin carbon fiber precursor	53:103	a composite cellulose-lignin carbon fiber precursor	53:103	Disassociated molecular orientation distributions of a composite cellulose-lignin carbon fiber precursor: A study by rotor synchronized NMR spectroscopy and X-ray scattering.					
33357862	6	63	theme	native	1031:1036	arg1	material					1054:1061	the native spruce wood raw material	1027:1061	the native spruce wood raw material	1027:1061	In contrast, the native spruce wood raw material displays both oriented lignin and cellulose.					
33357862	2	64	theme	light-weight	413:424	arg1	fibers					388:393	cellulose-lignin carbon fibers	364:393	cellulose-lignin carbon fibers	364:393	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
33357862	2	64	theme	light-weight	413:424	arg1	material					426:433	an attractive light-weight material	399:433	an attractive light-weight material for, e.g. wind power and automobile manufacturing	399:483	With the associated cost reduction, cellulose-lignin carbon fibers are an attractive light-weight material for, e.g. wind power and automobile manufacturing.					
32358696	6	0	theme	BG	972:973	arg1	loading					975:981	a BG loading	970:981	a BG loading of about 10% in the obtained scaffolds	970:1020	TGA analysis revealed a BG loading of about 10% in the obtained scaffolds.					
32358696	2	1	theme	metallic	338:345	arg1	ions					347:350	several metallic ions	330:350	several metallic ions (e.g., Ag, Sr, Cu, Nb)	330:373	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	10	2	theme	In	1369:1370	arg1	investigation					1389:1401	In vitro biological investigation	1369:1401	In vitro biological investigation	1369:1401	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	7	3	theme	simulated	1096:1104	arg1	fluid					1111:1115	simulated body fluid	1096:1115	simulated body fluid (SBF)	1096:1121	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	7	3	theme	simulated	1096:1104	arg1	SBF					1118:1120	SBF	1118:1120	SBF	1118:1120	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	3	4	theme	metal	464:468	arg1	ions					470:473	other metal ions	458:473	other metal ions	458:473	In particular, Nb, when compared with other metal ions, has been reported to be less cytotoxic and possess the ability to enhance mineralization process in human osteoblast populations.					
32358696	9	5	theme	FT-IR	1254:1258	arg1	peak					1260:1263	FT-IR peak	1254:1263	FT-IR peak around 603 cm-1	1254:1279	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	12	6	theme	engineering	1779:1789	arg1	applications					1791:1802	bone tissue engineering applications	1767:1802	bone tissue engineering applications	1767:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	5	7	with	scaffolds	853:861	arg1	porosity					879:886	a defined porosity	869:886	a defined porosity (15-90 μm)	869:897	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	5	7	with	scaffolds	853:861	arg1	μm					895:896	15-90 μm	889:896	15-90 μm	889:896	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	11	8	theme	%	1602:1602	arg1	lutea					1571:1575	S. lutea	1568:1575	S. lutea	1568:1575	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	11	8	theme	%	1602:1602	arg1	growth					1589:1594	E. coli growth	1581:1594	E. coli growth of ≈60% and ≈50%, respectively	1581:1625	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	4	9	theme	unloaded	801:808	arg1	regeneration					815:826	unloaded bone regeneration	801:826	unloaded bone regeneration	801:826	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	9	10	from	2θ = 32°	1297:1304	arg1	peak					1260:1263	FT-IR peak	1254:1263	FT-IR peak around 603 cm-1	1254:1279	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	9	10	from	2θ = 32°	1297:1304	arg1	peak					1289:1292	XRD peak	1285:1292	XRD peak at 2θ = 32°	1285:1304	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	6	11	theme	obtained	1003:1010	arg1	scaffolds					1012:1020	the obtained scaffolds	999:1020	the obtained scaffolds	999:1020	TGA analysis revealed a BG loading of about 10% in the obtained scaffolds.					
32358696	10	12	theme	biological	1378:1387	arg1	investigation					1389:1401	In vitro biological investigation	1369:1401	In vitro biological investigation	1369:1401	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	2	13	theme	BG	294:295	arg1	systems					297:303	BG systems	294:303	BG systems	294:303	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	1	14	theme	cell	195:198	arg1	functions					200:208	osteogenic cell functions	184:208	osteogenic cell functions	184:208	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	8	15	theme	SEM	1124:1126	arg1	observations					1128:1139	SEM observations	1124:1139	SEM observations	1124:1139	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	10	16	dep	In	1369:1370	arg1	vitro					1372:1376	vitro	1372:1376	vitro	1372:1376	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	8	17	dep	slow	1173:1176	arg1	days					1185:1188	21-28 days	1179:1188	21-28 days	1179:1188	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	12	18	theme	bone	1767:1770	arg1	applications					1791:1802	bone tissue engineering applications	1767:1802	bone tissue engineering applications	1767:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	11	19	dep	E.	1581:1582	arg1	coli					1584:1587	coli	1584:1587	coli	1584:1587	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	11	20	theme	S.	1568:1569	arg1	lutea					1571:1575	S. lutea	1568:1575	S. lutea	1568:1575	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	0	21	theme	Nb-doped	70:77	arg1	glass					89:93	Nb-doped bioactive glass	70:93	Nb-doped bioactive glass for bone tissue regeneration	70:122	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	5	22	with	dispersion	915:924	arg1	porosity					879:886	a defined porosity	869:886	a defined porosity (15-90 μm)	869:897	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	5	22	with	dispersion	915:924	arg1	μm					895:896	15-90 μm	889:896	15-90 μm	889:896	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	12	23	theme	promising	1706:1714	arg1	scaffolds					1693:1701	the produced composite scaffolds	1670:1701	the produced composite scaffolds	1670:1701	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	12	23	theme	promising	1706:1714	arg1	materials					1716:1724	promising materials	1706:1724	promising materials with inherent antibacterial activity for bone tissue engineering applications	1706:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	4	24	contain	containing	734:743	arg2	Nb					745:746	Nb	745:746	Nb	745:746	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	4	24	contain	containing	734:743	arg1	BG					731:732	a modified BG	720:732	a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration	720:826	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	2	25	dep	ions	347:350	arg1	Cu					367:368	Cu	367:368	Cu	367:368	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	2	25	dep	ions	347:350	arg1	Sr					363:364	Sr	363:364	Sr	363:364	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	2	25	dep	ions	347:350	arg1	Ag					359:360	Ag	359:360	Ag	359:360	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	2	25	dep	ions	347:350	arg1	Nb					371:372	Nb	371:372	Nb	371:372	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	0	26	theme	bone	99:102	arg1	regeneration					111:122	bone tissue regeneration	99:122	bone tissue regeneration	99:122	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	9	27	theme	phase	1342:1346	arg1	formation					1319:1327	the formation	1315:1327	the formation of a mineral phase after SBF immersion	1315:1366	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	12	28	theme	produced	1674:1681	arg1	scaffolds					1693:1701	the produced composite scaffolds	1670:1701	the produced composite scaffolds	1670:1701	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	12	28	theme	produced	1674:1681	arg1	materials					1716:1724	promising materials	1706:1724	promising materials with inherent antibacterial activity for bone tissue engineering applications	1706:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	5	29	theme	defined	871:877	arg1	porosity					879:886	a defined porosity	869:886	a defined porosity (15-90 μm)	869:897	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	5	29	theme	defined	871:877	arg1	μm					895:896	15-90 μm	889:896	15-90 μm	889:896	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	4	30	theme	electrophoretic	654:668	arg1	deposition					670:679	one-pot electrophoretic deposition	646:679	one-pot electrophoretic deposition (EPD)	646:685	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	4	30	theme	electrophoretic	654:668	arg1	EPD					682:684	EPD	682:684	EPD	682:684	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	10	31	theme	composite	1440:1448	arg1	scaffolds					1450:1458	composite scaffolds	1440:1458	composite scaffolds	1440:1458	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	0	32	theme	Electrophoretic	0:14	arg1	processing					16:25	Electrophoretic processing	0:25	Electrophoretic processing of chitosan	0:37	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	1	33	theme	unloaded	266:273	arg1	regeneration					280:291	loaded and unloaded bone regeneration	255:291	loaded and unloaded bone regeneration	255:291	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	5	34	theme	Self-standing	829:841	arg1	scaffolds					853:861	Self-standing composite scaffolds	829:861	Self-standing composite scaffolds	829:861	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	7	35	theme	apatite	1027:1033	arg1	ability					1045:1051	The apatite formation ability	1023:1051	The apatite formation ability of the scaffolds	1023:1068	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	12	36	with	materials	1716:1724	arg1	activity					1754:1761	inherent antibacterial activity	1731:1761	inherent antibacterial activity for bone tissue engineering applications	1731:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	12	37	theme	obtained	1644:1651	arg1	results					1653:1659	the obtained results	1640:1659	the obtained results	1640:1659	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	11	38	theme	Nb	1510:1511	arg1	scaffolds					1513:1521	BG-doped Nb scaffolds	1501:1521	BG-doped Nb scaffolds	1501:1521	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	8	39	theme	CaP	1219:1221	arg1	species					1223:1229	CaP species	1219:1229	CaP species	1219:1229	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	10	40	theme	cytotoxic	1467:1475	arg1	effects					1477:1483	no cytotoxic effects	1464:1483	no cytotoxic effects	1464:1483	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	2	41	theme	antibacterial	394:406	arg1	properties					408:417	antibacterial properties	394:417	antibacterial properties	394:417	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	3	42	theme	mineralization	550:563	arg1	process					565:571	mineralization process	550:571	mineralization process	550:571	In particular, Nb, when compared with other metal ions, has been reported to be less cytotoxic and possess the ability to enhance mineralization process in human osteoblast populations.					
32358696	2	43	theme	several	330:336	arg1	ions					347:350	several metallic ions	330:350	several metallic ions (e.g., Ag, Sr, Cu, Nb)	330:373	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	6	44	theme	%	994:994	arg1	loading					975:981	a BG loading	970:981	a BG loading of about 10% in the obtained scaffolds	970:1020	TGA analysis revealed a BG loading of about 10% in the obtained scaffolds.					
32358696	2	45	dep	Ag	359:360	arg1	e.g.					353:356	e.g.	353:356	e.g.	353:356	BG systems can be easily doped with several metallic ions (e.g., Ag, Sr, Cu, Nb) in order to confer antibacterial properties.					
32358696	3	46	theme	osteoblast	582:591	arg1	populations					593:603	human osteoblast populations	576:603	human osteoblast populations	576:603	In particular, Nb, when compared with other metal ions, has been reported to be less cytotoxic and possess the ability to enhance mineralization process in human osteoblast populations.					
32358696	1	47	theme	attractive	223:232	arg1	materials					234:242	attractive materials	223:242	attractive materials	223:242	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	6	48	from	loading	975:981	arg1	scaffolds					1012:1020	the obtained scaffolds	999:1020	the obtained scaffolds	999:1020	TGA analysis revealed a BG loading of about 10% in the obtained scaffolds.					
32358696	7	49	theme	body	1106:1109	arg1	fluid					1111:1115	simulated body fluid	1096:1115	simulated body fluid (SBF)	1096:1121	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	7	49	theme	body	1106:1109	arg1	SBF					1118:1120	SBF	1118:1120	SBF	1118:1120	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	1	50	theme	loaded	255:260	arg1	regeneration					280:291	loaded and unloaded bone regeneration	255:291	loaded and unloaded bone regeneration	255:291	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	10	51	contain	had	1460:1462	arg1	release					1421:1427	the release	1417:1427	the release of Nb from composite scaffolds	1417:1458	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	10	51	contain	had	1460:1462	arg2	effects					1477:1483	no cytotoxic effects	1464:1483	no cytotoxic effects	1464:1483	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	3	52	theme	other	458:462	arg1	ions					470:473	other metal ions	458:473	other metal ions	458:473	In particular, Nb, when compared with other metal ions, has been reported to be less cytotoxic and possess the ability to enhance mineralization process in human osteoblast populations.					
32358696	12	53	theme	tissue	1772:1777	arg1	applications					1791:1802	bone tissue engineering applications	1767:1802	bone tissue engineering applications	1767:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	4	54	theme	modified	722:729	arg1	BG					731:732	a modified BG	720:732	a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration	720:826	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	1	55	theme	osteogenic	184:193	arg1	functions					200:208	osteogenic cell functions	184:208	osteogenic cell functions	184:208	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	10	56	theme	Nb	1432:1433	arg1	release					1421:1427	the release	1417:1427	the release of Nb from composite scaffolds	1417:1458	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	9	57	theme	XRD	1285:1287	arg1	peak					1289:1292	XRD peak	1285:1292	XRD peak at 2θ = 32°	1285:1304	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	12	58	theme	antibacterial	1740:1752	arg1	activity					1754:1761	inherent antibacterial activity	1731:1761	inherent antibacterial activity for bone tissue engineering applications	1731:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	4	59	theme	bone	810:813	arg1	regeneration					815:826	unloaded bone regeneration	801:826	unloaded bone regeneration	801:826	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	7	60	theme	scaffolds	1060:1068	arg1	ability					1045:1051	The apatite formation ability	1023:1051	The apatite formation ability of the scaffolds	1023:1068	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	1	61	theme	Bioactive	125:133	arg1	BGs					144:146	BGs	144:146	BGs	144:146	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	1	61	theme	Bioactive	125:133	arg1	glasses					135:141	Bioactive glasses	125:141	Bioactive glasses (BGs)	125:147	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	11	62	theme	%	1611:1611	arg1	lutea					1571:1575	S. lutea	1568:1575	S. lutea	1568:1575	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	11	62	theme	%	1611:1611	arg1	growth					1589:1594	E. coli growth	1581:1594	E. coli growth of ≈60% and ≈50%, respectively	1581:1625	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	5	63	theme	BGs	929:931	arg1	scaffolds					853:861	Self-standing composite scaffolds	829:861	Self-standing composite scaffolds	829:861	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	5	63	theme	BGs	929:931	arg1	dispersion					915:924	homogeneous dispersion	903:924	homogeneous dispersion of BGs	903:931	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	4	64	theme	antibacterial	774:786	arg1	activity					788:795	antibacterial activity	774:795	antibacterial activity	774:795	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	0	65	theme	bioactive	79:87	arg1	glass					89:93	Nb-doped bioactive glass	70:93	Nb-doped bioactive glass for bone tissue regeneration	70:122	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	8	66	theme	FT-IR	1150:1154	arg1	spectra					1156:1162	FT-IR spectra	1150:1162	FT-IR spectra	1150:1162	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	1	67	theme	due	150:152	arg1	BGs					144:146	BGs	144:146	BGs	144:146	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	1	67	theme	due	150:152	arg1	glasses					135:141	Bioactive glasses	125:141	Bioactive glasses (BGs)	125:147	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	0	68	theme	tissue	104:109	arg1	regeneration					111:122	bone tissue regeneration	99:122	bone tissue regeneration	99:122	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	12	69	theme	inherent	1731:1738	arg1	activity					1754:1761	inherent antibacterial activity	1731:1761	inherent antibacterial activity for bone tissue engineering applications	1731:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	9	70	theme	mineral	1334:1340	arg1	phase					1342:1346	a mineral phase	1332:1346	a mineral phase after SBF immersion	1332:1366	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	8	71	theme	slow	1173:1176	arg1	nucleation					1205:1214	a slow (21-28 days) yet effective nucleation	1171:1214	a slow (21-28 days) yet effective nucleation of CaP species	1171:1229	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	11	72	theme	E.	1581:1582	arg1	growth					1589:1594	E. coli growth	1581:1594	E. coli growth of ≈60% and ≈50%, respectively	1581:1625	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	12	73	theme	composite	1683:1691	arg1	scaffolds					1693:1701	the produced composite scaffolds	1670:1701	the produced composite scaffolds	1670:1701	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	12	73	theme	composite	1683:1691	arg1	materials					1716:1724	promising materials	1706:1724	promising materials with inherent antibacterial activity for bone tissue engineering applications	1706:1802	Altogether, the obtained results disclose the produced composite scaffolds as promising materials with inherent antibacterial activity for bone tissue engineering applications.					
32358696	7	74	theme	formation	1035:1043	arg1	ability					1045:1051	The apatite formation ability	1023:1051	The apatite formation ability of the scaffolds	1023:1068	The apatite formation ability of the scaffolds was evaluated in vitro in simulated body fluid (SBF).					
32358696	6	75	theme	TGA	948:950	arg1	analysis					952:959	TGA analysis	948:959	TGA analysis	948:959	TGA analysis revealed a BG loading of about 10% in the obtained scaffolds.					
32358696	4	76	theme	one-pot	646:652	arg1	deposition					670:679	one-pot electrophoretic deposition	646:679	one-pot electrophoretic deposition (EPD)	646:685	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	4	76	theme	one-pot	646:652	arg1	EPD					682:684	EPD	682:684	EPD	682:684	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	0	77	theme	chitosan	30:37	arg1	processing					16:25	Electrophoretic processing	0:25	Electrophoretic processing of chitosan	0:37	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	1	78	theme	bone	275:278	arg1	regeneration					280:291	loaded and unloaded bone regeneration	255:291	loaded and unloaded bone regeneration	255:291	Bioactive glasses (BGs), due to their ability to influence osteogenic cell functions, have become attractive materials to improve loaded and unloaded bone regeneration.					
32358696	5	79	theme	composite	843:851	arg1	scaffolds					853:861	Self-standing composite scaffolds	829:861	Self-standing composite scaffolds	829:861	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	11	80	theme	BG-doped	1501:1508	arg1	scaffolds					1513:1521	BG-doped Nb scaffolds	1501:1521	BG-doped Nb scaffolds	1501:1521	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	8	81	theme	effective	1195:1203	arg1	nucleation					1205:1214	a slow (21-28 days) yet effective nucleation	1171:1214	a slow (21-28 days) yet effective nucleation of CaP species	1171:1229	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	0	82	theme	composite	45:53	arg1	scaffolds					55:63	composite scaffolds	45:63	composite scaffolds	45:63	Electrophoretic processing of chitosan based composite scaffolds with Nb-doped bioactive glass for bone tissue regeneration.					
32358696	4	83	with	substrates	758:767	arg1	activity					788:795	antibacterial activity	774:795	antibacterial activity	774:795	In this study, we co-deposited, through one-pot electrophoretic deposition (EPD), chitosan (CS), gelatin (GE) and a modified BG containing Nb to obtain substrates with antibacterial activity for unloaded bone regeneration.					
32358696	5	84	theme	homogeneous	903:913	arg1	dispersion					915:924	homogeneous dispersion	903:924	homogeneous dispersion of BGs	903:931	Self-standing composite scaffolds, with a defined porosity (15-90 μm) and homogeneous dispersion of BGs were obtained.					
32358696	11	85	theme	antibacterial	1533:1545	arg1	properties					1547:1556	antibacterial properties	1533:1556	antibacterial properties	1533:1556	Interestingly, BG-doped Nb scaffolds displayed antibacterial properties, reducing S. lutea and E. coli growth of ≈60% and ≈50%, respectively.					
32358696	8	86	theme	species	1223:1229	arg1	nucleation					1205:1214	a slow (21-28 days) yet effective nucleation	1171:1214	a slow (21-28 days) yet effective nucleation of CaP species	1171:1229	SEM observations, XRD and FT-IR spectra showed a slow (21-28 days) yet effective nucleation of CaP species on BGs.					
32358696	10	87	from	scaffolds	1450:1458	arg1	release					1421:1427	the release	1417:1427	the release of Nb from composite scaffolds	1417:1458	In vitro biological investigation revealed that the release of Nb from composite scaffolds had no cytotoxic effects.					
32358696	9	88	theme	SBF	1354:1356	arg1	immersion					1358:1366	SBF immersion	1354:1366	SBF immersion	1354:1366	In particular, FT-IR peak around 603 cm-1 and XRD peak at 2θ = 32°, denoted the formation of a mineral phase after SBF immersion.					
32358696	3	89	theme	human	576:580	arg1	populations					593:603	human osteoblast populations	576:603	human osteoblast populations	576:603	In particular, Nb, when compared with other metal ions, has been reported to be less cytotoxic and possess the ability to enhance mineralization process in human osteoblast populations.					
34534582	0	0	theme	starch-based	85:96	arg1	composite					98:106	starch-based composite	85:106	starch-based composite	85:106	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	2	1	from	framework	399:407	arg1	embedded					374:381	embedded	374:381	embedded	374:381	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	4	2	theme	Fe3O4	845:849	arg1	NPs					851:853	Fe3O4 NPs	845:853	Fe3O4 NPs	845:853	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	4	3	theme	TGA	672:674	arg1	XPS					656:658	the XPS	652:658	the XPS	652:658	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	4	3	theme	TGA	672:674	arg1	analyses					676:683	TGA analyses	672:683	TGA analyses	672:683	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	4	4	theme	Fe3+/2+	733:739	arg1	groups					758:763	Fe3+/2+ and Zn2+/hydroxy groups	733:763	Fe3+/2+ and Zn2+/hydroxy groups	733:763	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	7	5	from	separation	1156:1165	arg1	adsorption					1225:1234	practical adsorption	1215:1234	practical adsorption	1215:1234	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	4	6	theme	Zn2+/hydroxy	745:756	arg1	groups					758:763	Fe3+/2+ and Zn2+/hydroxy groups	733:763	Fe3+/2+ and Zn2+/hydroxy groups	733:763	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	5	7	theme	separation	918:927	arg1	behavior					929:936	an excellent separation behavior	905:936	an excellent separation behavior	905:936	Saturation magnetization value was measured with an excellent separation behavior.					
34534582	3	8	theme	micron	622:627	arg1	channels					629:636	micron channels	622:636	micron channels	622:636	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	4	9	theme	chains	793:798	arg1	recombination					773:785	the recombination	769:785	the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs	769:853	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	4	9	theme	chains	793:798	arg1	coordination					712:723	the coordination	708:723	the coordination between Fe3+/2+ and Zn2+/hydroxy groups	708:763	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	1	10	dep	Starch	145:150	arg1	St					153:154	St	153:154	St	153:154	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	0	11	theme	composite	98:106	arg1	fabrication					70:80	bioextruded fabrication	58:80	bioextruded fabrication of starch-based composite with hierarchical pore architecture	58:142	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	7	12	theme	bio-based	1116:1124	arg1	materials					1126:1134	bio-based materials	1116:1134	bio-based materials	1116:1134	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	0	13	with	fabrication	70:80	arg1	architecture					131:142	hierarchical pore architecture	113:142	hierarchical pore architecture	113:142	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	5	14	theme	excellent	908:916	arg1	behavior					929:936	an excellent separation behavior	905:936	an excellent separation behavior	905:936	Saturation magnetization value was measured with an excellent separation behavior.					
34534582	0	15	theme	pore	126:129	arg1	architecture					131:142	hierarchical pore architecture	113:142	hierarchical pore architecture	113:142	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	2	16	with	embedded	374:381	arg1	structure					434:442	hierarchical porous structure	414:442	hierarchical porous structure	414:442	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	4	17	theme	crystalline/amorphous	803:823	arg1	zones					825:829	crystalline/amorphous zones	803:829	crystalline/amorphous zones interfered by Fe3O4 NPs	803:853	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	4	18	from	coordination	712:723	arg1	zones					825:829	crystalline/amorphous zones	803:829	crystalline/amorphous zones interfered by Fe3O4 NPs	803:853	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	0	19	theme	hierarchical	113:124	arg1	architecture					131:142	hierarchical pore architecture	113:142	hierarchical pore architecture	113:142	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	0	20	theme	Bifunctional	0:11	arg1	Fe3O4					13:17	Bifunctional Fe3O4	0:17	Bifunctional Fe3O4	0:17	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	7	21	from	architecture	1189:1200	arg1	adsorption					1225:1234	practical adsorption	1215:1234	practical adsorption	1215:1234	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	7	22	theme	practical	1215:1223	arg1	adsorption					1225:1234	practical adsorption	1215:1234	practical adsorption	1215:1234	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	2	23	theme	porous	427:432	arg1	structure					434:442	hierarchical porous structure	414:442	hierarchical porous structure	414:442	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	6	24	theme	kinetic	945:951	arg1	equations					953:961	Seven kinetic equations	939:961	Seven kinetic equations	939:961	Seven kinetic equations were conducted for the fitting of dye adsorption data.					
34534582	2	25	theme	Fe3O4	291:295	arg1	NPs					297:299	Fe3O4 NPs	291:299	Fe3O4 NPs	291:299	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	7	26	theme	promising	1202:1210	arg1	architecture					1189:1200	hierarchical-pore architecture promising	1171:1210	hierarchical-pore architecture promising	1171:1210	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	2	27	theme	hierarchical	414:425	arg1	structure					434:442	hierarchical porous structure	414:442	hierarchical porous structure	414:442	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	1	28	theme	Zn-St-MOCP/nFe3O4	231:247	arg1	composite					249:257	Zn-St-MOCP/nFe3O4 composite	231:257	Zn-St-MOCP/nFe3O4 composite via bioextrusion	231:274	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	7	29	theme	continuous	1090:1099	arg1	fabrication					1101:1111	the continuous fabrication	1086:1111	the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption	1086:1234	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	1	30	theme	renewable	179:187	arg1	Starch					145:150	Starch	145:150	Starch	145:150	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	1	30	theme	renewable	179:187	arg1	%					201:201	> 80%	197:201	> 80%	197:201	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	1	30	theme	renewable	179:187	arg1	matrix					189:194	renewable matrix	179:194	renewable matrix (> 80%, db)	179:206	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	7	31	theme	hierarchical-pore	1171:1187	arg1	architecture					1189:1200	hierarchical-pore architecture promising	1171:1210	hierarchical-pore architecture promising	1171:1210	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	1	32	theme	composite	249:257	arg1	preparation					216:226	the preparation	212:226	the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion	212:274	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	7	33	theme	materials	1126:1134	arg1	fabrication					1101:1111	the continuous fabrication	1086:1111	the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption	1086:1234	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	2	34	theme	St-based	390:397	arg1	framework					399:407	the St-based framework	386:407	the St-based framework	386:407	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	7	35	with	fabrication	1101:1111	arg1	separation					1156:1165	rapid magnetic separation	1141:1165	rapid magnetic separation	1141:1165	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	7	35	with	fabrication	1101:1111	arg1	architecture					1189:1200	hierarchical-pore architecture promising	1171:1210	hierarchical-pore architecture promising	1171:1210	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	5	36	theme	Saturation	856:865	arg1	value					881:885	Saturation magnetization value	856:885	Saturation magnetization value	856:885	Saturation magnetization value was measured with an excellent separation behavior.					
34534582	3	37	theme	nFe3O4-induced	473:486	arg1	microstructure					488:501	the nFe3O4-induced microstructure	469:501	the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite	469:532	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	7	38	theme	bioextrusion	1047:1058	arg1	strategy					1060:1067	the nFe3O4-assisted bioextrusion strategy	1027:1067	the nFe3O4-assisted bioextrusion strategy	1027:1067	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	2	39	theme	SEM-EDS	448:454	arg1	mapping					456:462	SEM-EDS mapping	448:462	SEM-EDS mapping	448:462	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	1	40	dep	%	201:201	arg1	db					204:205	db	204:205	db	204:205	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	7	41	theme	rapid	1141:1145	arg1	separation					1156:1165	rapid magnetic separation	1141:1165	rapid magnetic separation	1141:1165	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	3	42	theme	Fe3O4	586:590	arg1	NPs					592:594	Fe3O4 NPs	586:594	Fe3O4 NPs onto the inner surface of micron channels	586:636	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	7	43	theme	magnetic	1147:1154	arg1	separation					1156:1165	rapid magnetic separation	1141:1165	rapid magnetic separation	1141:1165	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	7	44	theme	nFe3O4-assisted	1031:1045	arg1	strategy					1060:1067	the nFe3O4-assisted bioextrusion strategy	1027:1067	the nFe3O4-assisted bioextrusion strategy	1027:1067	Overall, the nFe3O4-assisted bioextrusion strategy is developed for the continuous fabrication of bio-based materials with rapid magnetic separation and hierarchical-pore architecture promising in practical adsorption.					
34534582	4	45	theme	St	790:791	arg1	chains					793:798	St chains	790:798	St chains	790:798	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	3	46	theme	channels	629:636	arg1	surface					611:617	the inner surface	601:617	the inner surface of micron channels	601:636	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	4	47	from	recombination	773:785	arg1	zones					825:829	crystalline/amorphous zones	803:829	crystalline/amorphous zones interfered by Fe3O4 NPs	803:853	According to the XPS, XRD, FTIR, TGA analyses, it is probably due to the coordination between Fe3+/2+ and Zn2+/hydroxy groups and the recombination of St chains in crystalline/amorphous zones interfered by Fe3O4 NPs.					
34534582	3	48	theme	submicronic	535:545	arg1	pores					547:551	submicronic pores	535:551	submicronic pores	535:551	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	1	49	used	used	161:164	arg2	matrix					189:194	renewable matrix	179:194	renewable matrix (> 80%, db)	179:206	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	1	49	used	used	161:164	arg2	green					169:173	green	169:173	green	169:173	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	1	49	used	used	161:164	arg2	Starch					145:150	Starch	145:150	Starch	145:150	Starch (St) was used as green and renewable matrix (> 80%, db) for the preparation of Zn-St-MOCP/nFe3O4 composite via bioextrusion.					
34534582	2	50	theme	NPs	297:299	arg1	Bifunction					277:286	Bifunction	277:286	Bifunction of Fe3O4 NPs as magnet and pore-inducer	277:326	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	6	51	theme	data	1012:1015	arg1	fitting					986:992	the fitting	982:992	the fitting of dye adsorption data	982:1015	Seven kinetic equations were conducted for the fitting of dye adsorption data.					
34534582	5	52	theme	magnetization	867:879	arg1	value					881:885	Saturation magnetization value	856:885	Saturation magnetization value	856:885	Saturation magnetization value was measured with an excellent separation behavior.					
34534582	3	53	theme	inner	605:609	arg1	surface					611:617	the inner surface	601:617	the inner surface of micron channels	601:636	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	2	54	from	embedded	374:381	arg1	framework					399:407	the St-based framework	386:407	the St-based framework	386:407	Bifunction of Fe3O4 NPs as magnet and pore-inducer was confirmed and could be more homogeneously embedded in the St-based framework with hierarchical porous structure via SEM-EDS mapping.					
34534582	3	55	theme	Zn-St-MOCP/nFe3O4	506:522	arg1	composite					524:532	Zn-St-MOCP/nFe3O4 composite	506:532	Zn-St-MOCP/nFe3O4 composite	506:532	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	6	56	theme	adsorption	1001:1010	arg1	data					1012:1015	dye adsorption data	997:1015	dye adsorption data	997:1015	Seven kinetic equations were conducted for the fitting of dye adsorption data.					
34534582	0	57	theme	bioextruded	58:68	arg1	fabrication					70:80	bioextruded fabrication	58:80	bioextruded fabrication of starch-based composite with hierarchical pore architecture	58:142	Bifunctional Fe3O4 nanoparticles as magnet and inducer in bioextruded fabrication of starch-based composite with hierarchical pore architecture.					
34534582	3	58	theme	composite	524:532	arg1	microstructure					488:501	the nFe3O4-induced microstructure	469:501	the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite	469:532	For the nFe3O4-induced microstructure of Zn-St-MOCP/nFe3O4 composite, submicronic pores and nanopores were observed with Fe3O4 NPs onto the inner surface of micron channels.					
34534582	6	59	theme	dye	997:999	arg1	data					1012:1015	dye adsorption data	997:1015	dye adsorption data	997:1015	Seven kinetic equations were conducted for the fitting of dye adsorption data.					
32108385	0	0	theme	boar	89:92	arg1	sperm					94:98	boar sperm	89:98	boar sperm	89:98	The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm.					
32108385	4	1	dep	significantly	847:859	arg1	p < .0001					862:870	p < .0001	862:870	p < .0001	862:870	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	3	2	theme	fatty	504:508	arg1	acids					510:514	saturated fatty acids	494:514	significantly higher saturated fatty acids (SFAs)	473:521	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	2	theme	fatty	504:508	arg1	SFAs					517:520	SFAs	517:520	SFAs	517:520	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	0	3	from	relationship	4:15	arg1	sperm					94:98	boar sperm	89:98	boar sperm	89:98	The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm.					
32108385	4	4	theme	blastocyst	810:819	arg1	formation					821:829	blastocyst formation	810:829	blastocyst formation	810:829	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	2	5	theme	membrane	338:345	arg1	integrity					347:355	plasma membrane integrity	331:355	plasma membrane integrity	331:355	The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05).					
32108385	5	6	theme	acrosome	1147:1154	arg1	reaction					1156:1163	the acrosome reaction	1143:1163	the acrosome reaction	1143:1163	Moreover, acrosome reaction was positively correlated with SFAs, whereas negatively correlated with PUFAs, of the PUFAs, the DPA (p = .0005) and DHA (p = <.0001) were negatively correlated with the acrosome reaction.					
32108385	4	7	theme	relative	909:916	arg1	sperm					903:907	acrosome-reacted sperm	886:907	acrosome-reacted sperm relative to non-acrosome-reacted sperm	886:946	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	8	theme	oocytes	834:840	arg1	cleavage					797:804	cleavage	797:804	cleavage	797:804	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	8	theme	oocytes	834:840	arg1	formation					821:829	blastocyst formation	810:829	blastocyst formation	810:829	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	3	9	theme	lower	527:531	arg1	composition					569:579	lower polyunsaturated fatty acids (PUFAs) composition	527:579	lower polyunsaturated fatty acids (PUFAs) composition	527:579	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	10	theme	acids	555:559	arg1	composition					569:579	lower polyunsaturated fatty acids (PUFAs) composition	527:579	lower polyunsaturated fatty acids (PUFAs) composition	527:579	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	4	11	dep	C22:5n-6	662:669	arg1	[DHA					745:748	docosahexaenoic acid [DHA	724:748	docosahexaenoic acid [DHA	724:748	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	11	dep	C22:5n-6	662:669	arg1	[DPA					694:697	docosapentaenoic acid [DPA]; p < .01)	672:708	docosapentaenoic acid [DPA]; p < .01)	672:708	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	11	dep	C22:5n-6	662:669	arg1	C22:6n-3					714:721	C22:6n-3	714:721	C22:6n-3	714:721	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	3	12	theme	polyunsaturated	533:547	arg1	acids					555:559	polyunsaturated fatty acids	533:559	lower polyunsaturated fatty acids (PUFAs) composition	527:579	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	12	theme	polyunsaturated	533:547	arg1	PUFAs					562:566	PUFAs	562:566	PUFAs	562:566	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	4	13	theme	non-acrosome-reacted	921:940	arg1	sperm					942:946	non-acrosome-reacted sperm	921:946	non-acrosome-reacted sperm	921:946	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	3	14	theme	higher	487:492	arg1	acids					510:514	saturated fatty acids	494:514	significantly higher saturated fatty acids (SFAs)	473:521	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	14	theme	higher	487:492	arg1	SFAs					517:520	SFAs	517:520	SFAs	517:520	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	6	15	theme	PUFAs	1208:1212	arg1	composition					1214:1224	the PUFAs composition	1204:1224	the PUFAs composition of sperm	1204:1233	Therefore, these results suggest that the PUFAs composition of sperm is closely involved in acrosome reaction in pigs.					
32108385	4	16	theme	acid	740:743	arg1	[DHA					745:748	docosahexaenoic acid [DHA	724:748	docosahexaenoic acid [DHA	724:748	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	16	theme	acid	740:743	arg1	[DPA					694:697	docosapentaenoic acid [DPA]; p < .01)	672:708	docosapentaenoic acid [DPA]; p < .01)	672:708	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	1	17	theme	acid	179:182	arg1	composition					184:194	fatty acid composition	173:194	fatty acid composition	173:194	This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm.					
32108385	4	18	theme	docosahexaenoic	724:738	arg1	acid					740:743	docosahexaenoic acid	724:743	docosahexaenoic acid [DHA	724:748	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	19	dep	[DPA	694:697	arg1	p < .0001					752:760	p < .0001	752:760	p < .0001	752:760	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	6	20	theme	sperm	1229:1233	arg1	composition					1214:1224	the PUFAs composition	1204:1224	the PUFAs composition of sperm	1204:1233	Therefore, these results suggest that the PUFAs composition of sperm is closely involved in acrosome reaction in pigs.					
32108385	0	21	theme	acrosome	25:32	arg1	reaction					34:41	acrosome reaction	25:41	acrosome reaction	25:41	The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm.					
32108385	6	22	from	reaction	1267:1274	arg1	pigs					1279:1282	pigs	1279:1282	pigs	1279:1282	Therefore, these results suggest that the PUFAs composition of sperm is closely involved in acrosome reaction in pigs.					
32108385	3	23	theme	fatty	549:553	arg1	acids					555:559	polyunsaturated fatty acids	533:559	lower polyunsaturated fatty acids (PUFAs) composition	527:579	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	23	theme	fatty	549:553	arg1	PUFAs					562:566	PUFAs	562:566	PUFAs	562:566	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	2	24	theme	boar	414:417	arg1	sperm					419:423	boar sperm	414:423	boar sperm	414:423	The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05).					
32108385	1	25	theme	fatty	173:177	arg1	composition					184:194	fatty acid composition	173:194	fatty acid composition	173:194	This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm.					
32108385	4	26	theme	docosapentaenoic	672:687	arg1	[DHA					745:748	docosahexaenoic acid [DHA	724:748	docosahexaenoic acid [DHA	724:748	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	26	theme	docosapentaenoic	672:687	arg1	[DPA					694:697	docosapentaenoic acid [DPA]; p < .01)	672:708	docosapentaenoic acid [DPA]; p < .01)	672:708	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	27	theme	acrosome-reacted	886:901	arg1	sperm					903:907	acrosome-reacted sperm	886:907	acrosome-reacted sperm relative to non-acrosome-reacted sperm	886:946	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	2	28	theme	acrosome	241:248	arg1	reaction					250:257	The acrosome reaction	237:257	The acrosome reaction	237:257	The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05).					
32108385	1	29	theme	boar	225:228	arg1	sperm					230:234	boar sperm	225:234	boar sperm	225:234	This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm.					
32108385	0	30	theme	fatty	63:67	arg1	composition					74:84	polyunsaturated fatty acid composition	47:84	polyunsaturated fatty acid composition	47:84	The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm.					
32108385	6	31	theme	acrosome	1258:1265	arg1	reaction					1267:1274	acrosome reaction	1258:1274	acrosome reaction in pigs	1258:1282	Therefore, these results suggest that the PUFAs composition of sperm is closely involved in acrosome reaction in pigs.					
32108385	2	32	theme	plasma	331:336	arg1	integrity					347:355	plasma membrane integrity	331:355	plasma membrane integrity	331:355	The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05).					
32108385	0	33	theme	polyunsaturated	47:61	arg1	composition					74:84	polyunsaturated fatty acid composition	47:84	polyunsaturated fatty acid composition	47:84	The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm.					
32108385	3	34	theme	saturated	494:502	arg1	acids					510:514	saturated fatty acids	494:514	significantly higher saturated fatty acids (SFAs)	473:521	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	34	theme	saturated	494:502	arg1	SFAs					517:520	SFAs	517:520	SFAs	517:520	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	4	35	theme	acid	689:692	arg1	[DHA					745:748	docosahexaenoic acid [DHA	724:748	docosahexaenoic acid [DHA	724:748	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	4	35	theme	acid	689:692	arg1	[DPA					694:697	docosapentaenoic acid [DPA]; p < .01)	672:708	docosapentaenoic acid [DPA]; p < .01)	672:708	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	5	36	theme	acrosome	959:966	arg1	reaction					968:975	acrosome reaction	959:975	acrosome reaction	959:975	Moreover, acrosome reaction was positively correlated with SFAs, whereas negatively correlated with PUFAs, of the PUFAs, the DPA (p = .0005) and DHA (p = <.0001) were negatively correlated with the acrosome reaction.					
32108385	3	37	theme	reaction	610:617	arg1	p < .0001					626:634	p < .0001	626:634	p < .0001	626:634	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	37	theme	reaction	610:617	arg1	group					619:623	the non-acrosome reaction group	593:623	the non-acrosome reaction group (p < .0001)	593:635	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	1	38	from	fertility	212:220	arg1	sperm					230:234	boar sperm	225:234	boar sperm	225:234	This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm.					
32108385	4	39	dep	PUFAs	655:659	arg1	C22:5n-6					662:669	C22:5n-6	662:669	C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001)	662:761	In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm.					
32108385	2	40	dep	reduced	375:381	arg1	p < .05					426:432	p < .05	426:432	p < .05	426:432	The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05).					
32108385	2	41	theme	MBCD	400:403	arg1	level					405:409	the MBCD level	396:409	the MBCD level	396:409	The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05).					
32108385	3	42	theme	acrosome-reacted	440:455	arg1	sperm					457:461	The acrosome-reacted sperm	436:461	The acrosome-reacted sperm	436:461	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	1	43	theme	acrosome	150:157	arg1	reactions					159:167	acrosome reactions	150:167	acrosome reactions	150:167	This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm.					
32108385	5	44	dep	PUFAs	1049:1053	arg1	correlated					1127:1136	correlated	1127:1136	were negatively correlated with the acrosome reaction	1111:1163	Moreover, acrosome reaction was positively correlated with SFAs, whereas negatively correlated with PUFAs, of the PUFAs, the DPA (p = .0005) and DHA (p = <.0001) were negatively correlated with the acrosome reaction.					
32108385	0	45	theme	acid	69:72	arg1	composition					74:84	polyunsaturated fatty acid composition	47:84	polyunsaturated fatty acid composition	47:84	The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm.					
32108385	3	46	theme	non-acrosome	597:608	arg1	p < .0001					626:634	p < .0001	626:634	p < .0001	626:634	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
32108385	3	46	theme	non-acrosome	597:608	arg1	group					619:623	the non-acrosome reaction group	593:623	the non-acrosome reaction group (p < .0001)	593:635	The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001).					
33438606	0	0	from	ability	27:33	arg1	scaffolds					140:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	5	1	from	potentiality	912:923	arg1	applications					1004:1015	bone tissue engineering applications	980:1015	bone tissue engineering applications	980:1015	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	3	2	theme	in-vitro	538:545	arg1	biocompatibility					547:562	in-vitro biocompatibility	538:562	in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation	538:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	3	from	scaffolds	580:588	arg1	terms					593:597	terms	593:597	terms of cell viability, cell attachment and proliferation	593:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	5	4	theme	dried	951:955	arg1	scaffolds					967:975	prepared porous freeze dried composite scaffolds	928:975	prepared porous freeze dried composite scaffolds	928:975	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	4	5	theme	osteogenic	733:742	arg1	assay					760:764	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	0	6	theme	/nano	98:102	arg1	scaffolds					140:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	3	7	theme	cell	618:621	arg1	attachment					623:632	cell attachment	618:632	cell attachment	618:632	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	8	from	terms	593:597	arg1	biocompatibility					547:562	in-vitro biocompatibility	538:562	in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation	538:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	1	9	theme	porous	283:288	arg1	scaffolds					300:308	Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds	208:308	Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds	208:308	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	5	10	theme	engineering	992:1002	arg1	applications					1004:1015	bone tissue engineering applications	980:1015	bone tissue engineering applications	980:1015	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	4	11	theme	calcium	812:818	arg1	content					820:826	Total calcium content	806:826	Total calcium content	806:826	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	4	12	theme	differentiation	744:758	arg1	assay					760:764	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	1	13	theme	composite	290:298	arg1	scaffolds					300:308	Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds	208:308	Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds	208:308	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	0	14	theme	Tricalcium	109:118	arg1	scaffolds					140:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	5	15	theme	scaffolds	967:975	arg1	potentiality					912:923	the potentiality	908:923	the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications	908:1015	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	3	16	theme	prepared	571:578	arg1	scaffolds					580:588	the prepared scaffolds	567:588	the prepared scaffolds in terms of cell viability, cell attachment and proliferation	567:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	17	theme	mesenchymal	485:495	arg1	hMSCs					509:513	hMSCs	509:513	hMSCs	509:513	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	17	theme	mesenchymal	485:495	arg1	cells					502:506	Human mesenchymal stem cells	479:506	Human mesenchymal stem cells (hMSCs)	479:514	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	0	18	theme	beta	104:107	arg1	scaffolds					140:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	5	19	theme	composite	957:965	arg1	scaffolds					967:975	prepared porous freeze dried composite scaffolds	928:975	prepared porous freeze dried composite scaffolds	928:975	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	3	20	theme	scaffolds	580:588	arg1	biocompatibility					547:562	in-vitro biocompatibility	538:562	in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation	538:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	21	theme	stem	497:500	arg1	hMSCs					509:513	hMSCs	509:513	hMSCs	509:513	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	21	theme	stem	497:500	arg1	cells					502:506	Human mesenchymal stem cells	479:506	Human mesenchymal stem cells (hMSCs)	479:514	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	0	22	theme	differentiation	11:25	arg1	ability					27:33	Osteogenic differentiation ability	0:33	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.	0:149	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	3	23	from	biocompatibility	547:562	arg1	terms					593:597	terms	593:597	terms of cell viability, cell attachment and proliferation	593:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	24	theme	viability	607:615	arg1	terms					593:597	terms	593:597	terms of cell viability, cell attachment and proliferation	593:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	2	25	theme	compressive	415:425	arg1	strength					427:434	the compressive strength	411:434	the compressive strength besides decelerating the degradation rate	411:476	Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate.					
33438606	0	26	theme	Osteogenic	0:9	arg1	ability					27:33	Osteogenic differentiation ability	0:33	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.	0:149	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	1	27	theme	scaffolds	300:308	arg1	characterization					188:203	characterization	188:203	characterization	188:203	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	1	27	theme	scaffolds	300:308	arg1	development					172:182	development	172:182	development	172:182	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	4	28	theme	Phosphatase	784:794	arg1	assay					796:800	Alkaline Phosphatase assay	775:800	Alkaline Phosphatase assay	775:800	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	4	29	dep	assay	760:764	arg1	results					766:772	results	766:772	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	4	29	dep	assay	760:764	arg1	assay					796:800	Alkaline Phosphatase assay	775:800	Alkaline Phosphatase assay	775:800	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	4	29	dep	assay	760:764	arg1	content					820:826	Total calcium content	806:826	Total calcium content	806:826	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	3	30	theme	Human	479:483	arg1	hMSCs					509:513	hMSCs	509:513	hMSCs	509:513	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	30	theme	Human	479:483	arg1	cells					502:506	Human mesenchymal stem cells	479:506	Human mesenchymal stem cells (hMSCs)	479:514	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	0	31	theme	composite	130:138	arg1	scaffolds					140:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	5	32	theme	tissue	985:990	arg1	applications					1004:1015	bone tissue engineering applications	980:1015	bone tissue engineering applications	980:1015	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	4	33	theme	Total	806:810	arg1	content					820:826	Total calcium content	806:826	Total calcium content	806:826	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	1	34	theme	freeze	313:318	arg1	method					327:332	freeze drying method	313:332	freeze drying method	313:332	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	0	35	theme	Phosphate	120:128	arg1	scaffolds					140:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds	70:148	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	3	36	theme	DNA	674:676	arg1	assays					693:698	DNA Quantification assays	674:698	DNA Quantification assays	674:698	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	3	37	theme	cell	602:605	arg1	viability					607:615	cell viability	602:615	cell viability	602:615	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	2	38	theme	degradation	461:471	arg1	rate					473:476	the degradation rate	457:476	decelerating the degradation rate	444:476	Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate.					
33438606	0	39	theme	mesenchymal	44:54	arg1	cells					61:65	human mesenchymal stem cells	38:65	human mesenchymal stem cells	38:65	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	1	40	theme	drying	320:325	arg1	method					327:332	freeze drying method	313:332	freeze drying method	313:332	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	3	41	theme	Quantification	678:691	arg1	assays					693:698	DNA Quantification assays	674:698	DNA Quantification assays	674:698	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	4	42	theme	composite	859:867	arg1	scaffolds					869:877	composite scaffolds	859:877	composite scaffolds	859:877	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	5	43	theme	prepared	928:935	arg1	scaffolds					967:975	prepared porous freeze dried composite scaffolds	928:975	prepared porous freeze dried composite scaffolds	928:975	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	0	44	theme	human	38:42	arg1	cells					61:65	human mesenchymal stem cells	38:65	human mesenchymal stem cells	38:65	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	2	45	dep	decelerating	444:455	arg1	rate					473:476	the degradation rate	457:476	decelerating the degradation rate	444:476	Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate.					
33438606	0	46	theme	cells	61:65	arg1	ability					27:33	Osteogenic differentiation ability	0:33	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.	0:149	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	5	47	theme	porous	937:942	arg1	scaffolds					967:975	prepared porous freeze dried composite scaffolds	928:975	prepared porous freeze dried composite scaffolds	928:975	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	1	48	dep	development	172:182	arg1	the					168:170	the	168:170	the	168:170	Our work depicts the development and characterization of Chitosan/Poly (caprolactone)/nano beta-Tricalcium phosphate (CS/PCL/β-TCP) porous composite scaffolds by freeze drying method.					
33438606	2	49	theme	composite	363:371	arg1	scaffolds					373:381	CS/β-TCP composite scaffolds	354:381	CS/β-TCP composite scaffolds	354:381	Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate.					
33438606	0	50	theme	stem	56:59	arg1	cells					61:65	human mesenchymal stem cells	38:65	human mesenchymal stem cells	38:65	Osteogenic differentiation ability of human mesenchymal stem cells on Chitosan/Poly (Caprolactone)/nano beta Tricalcium Phosphate composite scaffolds.					
33438606	3	51	theme	attachment	623:632	arg1	terms					593:597	terms	593:597	terms of cell viability, cell attachment and proliferation	593:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	2	52	theme	CS/β-TCP	354:361	arg1	scaffolds					373:381	CS/β-TCP composite scaffolds	354:381	CS/β-TCP composite scaffolds	354:381	Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate.					
33438606	3	53	theme	proliferation	638:650	arg1	terms					593:597	terms	593:597	terms of cell viability, cell attachment and proliferation	593:650	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	4	54	theme	Alkaline	775:782	arg1	Phosphatase					784:794	Alkaline Phosphatase	775:794	Alkaline Phosphatase assay	775:800	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	2	55	theme	PCL	347:349	arg1	Addition					335:342	Addition	335:342	Addition of PCL to CS/β-TCP composite scaffolds	335:381	Addition of PCL to CS/β-TCP composite scaffolds had significantly increased the compressive strength besides decelerating the degradation rate.					
33438606	5	56	theme	bone	980:983	arg1	applications					1004:1015	bone tissue engineering applications	980:1015	bone tissue engineering applications	980:1015	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33438606	4	57	theme	β-TCP	850:854	arg1	role					842:845	the role	838:845	the role of β-TCP in composite scaffolds	838:877	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	4	58	theme	increased	723:731	arg1	assay					760:764	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content)	723:827	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	3	59	theme	MTT	655:657	arg1	assay					659:663	MTT assay	655:663	MTT assay	655:663	Human mesenchymal stem cells (hMSCs) were chosen to assess in-vitro biocompatibility of the prepared scaffolds in terms of cell viability, cell attachment and proliferation by MTT assay, SEM and DNA Quantification assays respectively.					
33438606	4	60	from	role	842:845	arg1	scaffolds					869:877	composite scaffolds	859:877	composite scaffolds	859:877	Further, increased osteogenic differentiation assay results (Alkaline Phosphatase assay and Total calcium content) revealed the role of β-TCP in composite scaffolds.					
33438606	5	61	theme	freeze	944:949	arg1	scaffolds					967:975	prepared porous freeze dried composite scaffolds	928:975	prepared porous freeze dried composite scaffolds	928:975	Altogether, results suggest the potentiality of prepared porous freeze dried composite scaffolds in bone tissue engineering applications.					
33428954	0	0	theme	gastric	98:104	arg1	stability					106:114	gastric stability	98:114	gastric stability	98:114	Development and statistical optimization of alginate-Neusilin US2 micro-composite beads to elicit gastric stability and sustained action of hesperidin.					
33428954	6	1	dep	characterized	877:889	arg1	OF					868:869	OF	868:869	OF	868:869	The optimized beads (OF) were characterized for their morphology and size by motic microscopy and scanning electron microscopy.					
33428954	1	2	theme	micro-composite	178:192	arg1	beads					199:203	The Alginate-Neusilin US2 micro-composite (MC) beads	152:203	The Alginate-Neusilin US2 micro-composite (MC) beads	152:203	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
33428954	8	3	from	stomach	1260:1266	arg1	degradation					1234:1244	acidic degradation	1227:1244	acidic degradation of HES in the stomach	1227:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	7	4	theme	vitro	978:982	arg1	release					984:990	vitro release	978:990	vitro release	978:990	In vitro release, kinetic studies were performed in simulated gastric and intestinal fluids.					
33428954	3	5	theme	software	607:614	arg1	13.2.1					620:625	statistical software JMP 13.2.1	595:625	statistical software JMP 13.2.1	595:625	Nine batches were prepared by formulation design employing statistical software JMP 13.2.1.					
33428954	7	6	theme	simulated	1027:1035	arg1	fluids					1060:1065	simulated gastric and intestinal fluids	1027:1065	simulated gastric and intestinal fluids	1027:1065	In vitro release, kinetic studies were performed in simulated gastric and intestinal fluids.					
33428954	5	7	theme	factors	809:815	arg1	concentration					792:804	the optimum concentration	780:804	the optimum concentration of factors based on desirable responses	780:844	Further, a prediction profiler was used to trace the optimum concentration of factors based on desirable responses.					
33428954	8	8	theme	In	1068:1069	arg1	studies					1092:1098	In vivo pharmacokinetic studies	1068:1098	In vivo pharmacokinetic studies	1068:1098	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	3	9	theme	JMP	616:618	arg1	13.2.1					620:625	statistical software JMP 13.2.1	595:625	statistical software JMP 13.2.1	595:625	Nine batches were prepared by formulation design employing statistical software JMP 13.2.1.					
33428954	2	10	theme	swelling	514:521	arg1	degree					523:528	swelling degree	514:528	swelling degree (Y3)	514:533	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	10	theme	swelling	514:521	arg1	Y3					531:532	Y3	531:532	Y3	531:532	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	11	theme	factorial	284:292	arg1	design					294:299	A 32 full factorial design	274:299	A 32 full factorial design	274:299	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	10	12	from	alternative	1461:1471	arg1	nutshell					1390:1397	a nutshell	1388:1397	a nutshell	1388:1397	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	9	13	theme	formulation	1300:1310	arg1	3.86 years					1328:1337	3.86 years	1328:1337	3.86 years suggested better stability after fabrication	1328:1382	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	9	13	theme	formulation	1300:1310	arg1	life					1289:1292	The estimated shelf life	1269:1292	The estimated shelf life of OF formulation	1269:1310	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	8	14	theme	acidic	1227:1232	arg1	degradation					1234:1244	acidic degradation	1227:1244	acidic degradation of HES in the stomach	1227:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	2	15	theme	full	279:282	arg1	design					294:299	A 32 full factorial design	274:299	A 32 full factorial design	274:299	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	8	16	theme	pharmacokinetic	1076:1090	arg1	studies					1092:1098	In vivo pharmacokinetic studies	1068:1098	In vivo pharmacokinetic studies	1068:1098	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	4	17	theme	multiple	632:639	arg1	MLRA					662:665	MLRA	662:665	MLRA	662:665	The multiple regression analysis (MLRA) was carried to explore the influence of factor over responses.					
33428954	4	17	theme	multiple	632:639	arg1	analysis					652:659	The multiple regression analysis	628:659	The multiple regression analysis (MLRA)	628:666	The multiple regression analysis (MLRA) was carried to explore the influence of factor over responses.					
33428954	7	18	theme	kinetic	993:999	arg1	studies					1001:1007	kinetic studies	993:1007	kinetic studies	993:1007	In vitro release, kinetic studies were performed in simulated gastric and intestinal fluids.					
33428954	6	19	theme	scanning	945:952	arg1	microscopy					963:972	scanning electron microscopy	945:972	scanning electron microscopy	945:972	The optimized beads (OF) were characterized for their morphology and size by motic microscopy and scanning electron microscopy.					
33428954	8	20	theme	HES	1249:1251	arg1	degradation					1234:1244	acidic degradation	1227:1244	acidic degradation of HES in the stomach	1227:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	8	21	theme	better	1109:1114	arg1	absorption					1116:1125	better absorption	1109:1125	better absorption of HES from optimized beads	1109:1153	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	2	22	theme	particle	462:469	arg1	size					471:474	particle size	462:474	particle size (Y1)	462:479	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	22	theme	particle	462:469	arg1	Y1					477:478	Y1	477:478	Y1	477:478	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	7	23	theme	intestinal	1049:1058	arg1	fluids					1060:1065	simulated gastric and intestinal fluids	1027:1065	simulated gastric and intestinal fluids	1027:1065	In vitro release, kinetic studies were performed in simulated gastric and intestinal fluids.					
33428954	10	24	theme	oral	1487:1490	arg1	delivery					1502:1509	promising oral sustained delivery	1477:1509	promising oral sustained delivery of HES	1477:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	10	25	theme	micro-composite	1414:1428	arg1	alternative					1461:1471	a better alternative	1452:1471	a better alternative for promising oral sustained delivery of HES	1452:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	10	25	theme	micro-composite	1414:1428	arg1	beads					1430:1434	the developed micro-composite beads	1400:1434	the developed micro-composite beads of HES	1400:1441	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	2	26	theme	alginate	382:389	arg1	concentration					358:370	the concentration	354:370	the concentration of sodium alginate (X1), and Neusilin US2 (X2)	354:417	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	10	27	theme	promising	1477:1485	arg1	delivery					1502:1509	promising oral sustained delivery	1477:1509	promising oral sustained delivery of HES	1477:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	10	28	theme	sustained	1492:1500	arg1	delivery					1502:1509	promising oral sustained delivery	1477:1509	promising oral sustained delivery of HES	1477:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	2	29	theme	independent	314:324	arg1	factors					337:343	factors	337:343	factors	337:343	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	29	theme	independent	314:324	arg1	variables					326:334	independent variables	314:334	independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2)	314:417	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	30	theme	sodium	375:380	arg1	X2					415:416	X2	415:416	X2	415:416	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	30	theme	sodium	375:380	arg1	X1					392:393	X1	392:393	X1	392:393	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	30	theme	sodium	375:380	arg1	alginate					382:389	sodium alginate	375:389	sodium alginate (X1)	375:394	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	0	31	theme	hesperidin	140:149	arg1	action					130:135	action	130:135	action of hesperidin	130:149	Development and statistical optimization of alginate-Neusilin US2 micro-composite beads to elicit gastric stability and sustained action of hesperidin.					
33428954	8	32	theme	HES	1130:1132	arg1	absorption					1116:1125	better absorption	1109:1125	better absorption of HES from optimized beads	1109:1153	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	2	33	theme	Neusilin	401:408	arg1	US2					410:412	Neusilin US2	401:412	Neusilin US2	401:412	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	34	theme	US2	410:412	arg1	concentration					358:370	the concentration	354:370	the concentration of sodium alginate (X1), and Neusilin US2 (X2)	354:417	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	10	35	theme	developed	1404:1412	arg1	alternative					1461:1471	a better alternative	1452:1471	a better alternative for promising oral sustained delivery of HES	1452:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	10	35	theme	developed	1404:1412	arg1	beads					1430:1434	the developed micro-composite beads	1400:1434	the developed micro-composite beads of HES	1400:1441	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	2	36	theme	entrapment	482:491	arg1	Y2					505:506	Y2	505:506	Y2	505:506	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	36	theme	entrapment	482:491	arg1	efficiency					493:502	entrapment efficiency	482:502	entrapment efficiency (Y2)	482:507	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	10	37	theme	HES	1514:1516	arg1	delivery					1502:1509	promising oral sustained delivery	1477:1509	promising oral sustained delivery of HES	1477:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	5	38	used	used	766:769	arg2	profiler					753:760	a prediction profiler	740:760	a prediction profiler	740:760	Further, a prediction profiler was used to trace the optimum concentration of factors based on desirable responses.					
33428954	5	39	theme	prediction	742:751	arg1	profiler					753:760	a prediction profiler	740:760	a prediction profiler	740:760	Further, a prediction profiler was used to trace the optimum concentration of factors based on desirable responses.					
33428954	0	40	theme	alginate-Neusilin	44:60	arg1	US2					62:64	alginate-Neusilin US2	44:64	alginate-Neusilin US2	44:64	Development and statistical optimization of alginate-Neusilin US2 micro-composite beads to elicit gastric stability and sustained action of hesperidin.					
33428954	6	41	theme	motic	924:928	arg1	microscopy					930:939	motic microscopy	924:939	motic microscopy	924:939	The optimized beads (OF) were characterized for their morphology and size by motic microscopy and scanning electron microscopy.					
33428954	8	42	theme	HES	1172:1174	arg1	suspension					1176:1185	HES suspension	1172:1185	HES suspension which could be due to the prevention of acidic degradation of HES in the stomach	1172:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	2	43	theme	dependant	423:431	arg1	response					444:451	response	444:451	response	444:451	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	43	theme	dependant	423:431	arg1	variables					433:441	dependant variables	423:441	dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3)	423:533	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	5	44	theme	optimum	784:790	arg1	concentration					792:804	the optimum concentration	780:804	the optimum concentration of factors based on desirable responses	780:844	Further, a prediction profiler was used to trace the optimum concentration of factors based on desirable responses.					
33428954	10	45	theme	better	1454:1459	arg1	alternative					1461:1471	a better alternative	1452:1471	a better alternative for promising oral sustained delivery of HES	1452:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	10	45	theme	better	1454:1459	arg1	beads					1430:1434	the developed micro-composite beads	1400:1434	the developed micro-composite beads of HES	1400:1441	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	2	46	theme	such	346:349	arg1	factors					337:343	factors	337:343	factors	337:343	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	2	46	theme	such	346:349	arg1	variables					326:334	independent variables	314:334	independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2)	314:417	A 32 full factorial design encompassing independent variables (factors) such as the concentration of sodium alginate (X1), and Neusilin US2 (X2) and dependant variables (response) such as particle size (Y1), entrapment efficiency (Y2), and swelling degree (Y3).					
33428954	0	47	theme	US2	62:64	arg1	Development					0:10	Development	0:10	Development	0:10	Development and statistical optimization of alginate-Neusilin US2 micro-composite beads to elicit gastric stability and sustained action of hesperidin.					
33428954	0	47	theme	US2	62:64	arg1	optimization					28:39	statistical optimization	16:39	statistical optimization of alginate-Neusilin US2	16:64	Development and statistical optimization of alginate-Neusilin US2 micro-composite beads to elicit gastric stability and sustained action of hesperidin.					
33428954	9	48	theme	better	1349:1354	arg1	stability					1356:1364	better stability	1349:1364	better stability	1349:1364	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	8	49	dep	In	1068:1069	arg1	vivo					1071:1074	vivo	1071:1074	vivo	1071:1074	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	8	50	from	HES	1249:1251	arg1	stomach					1260:1266	the stomach	1256:1266	the stomach	1256:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	4	51	theme	factor	708:713	arg1	influence					695:703	the influence	691:703	the influence of factor over responses	691:728	The multiple regression analysis (MLRA) was carried to explore the influence of factor over responses.					
33428954	8	52	dep	revealed	1100:1107	arg1	OF					1156:1157	OF	1156:1157	OF	1156:1157	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	3	53	theme	statistical	595:605	arg1	13.2.1					620:625	statistical software JMP 13.2.1	595:625	statistical software JMP 13.2.1	595:625	Nine batches were prepared by formulation design employing statistical software JMP 13.2.1.					
33428954	8	54	from	beads	1149:1153	arg1	absorption					1116:1125	better absorption	1109:1125	better absorption of HES from optimized beads	1109:1153	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	5	55	theme	desirable	826:834	arg1	responses					836:844	desirable responses	826:844	desirable responses	826:844	Further, a prediction profiler was used to trace the optimum concentration of factors based on desirable responses.					
33428954	1	56	theme	oral	239:242	arg1	delivery					244:251	oral delivery	239:251	oral delivery of hesperidin (HES)	239:271	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
33428954	3	57	theme	formulation	566:576	arg1	design					578:583	formulation design	566:583	formulation design employing statistical software JMP 13.2.1	566:625	Nine batches were prepared by formulation design employing statistical software JMP 13.2.1.					
33428954	8	58	theme	degradation	1234:1244	arg1	prevention					1213:1222	the prevention	1209:1222	the prevention of acidic degradation of HES in the stomach	1209:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	4	59	theme	regression	641:650	arg1	MLRA					662:665	MLRA	662:665	MLRA	662:665	The multiple regression analysis (MLRA) was carried to explore the influence of factor over responses.					
33428954	4	59	theme	regression	641:650	arg1	analysis					652:659	The multiple regression analysis	628:659	The multiple regression analysis (MLRA)	628:666	The multiple regression analysis (MLRA) was carried to explore the influence of factor over responses.					
33428954	8	60	theme	optimized	1139:1147	arg1	beads					1149:1153	optimized beads	1139:1153	optimized beads	1139:1153	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	9	61	theme	estimated	1273:1281	arg1	3.86 years					1328:1337	3.86 years	1328:1337	3.86 years suggested better stability after fabrication	1328:1382	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	9	61	theme	estimated	1273:1281	arg1	life					1289:1292	The estimated shelf life	1269:1292	The estimated shelf life of OF formulation	1269:1310	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	10	62	theme	HES	1439:1441	arg1	alternative					1461:1471	a better alternative	1452:1471	a better alternative for promising oral sustained delivery of HES	1452:1516	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	10	62	theme	HES	1439:1441	arg1	beads					1430:1434	the developed micro-composite beads	1400:1434	the developed micro-composite beads of HES	1400:1441	In a nutshell, the developed micro-composite beads of HES could be a better alternative for promising oral sustained delivery of HES.					
33428954	6	63	theme	electron	954:961	arg1	microscopy					963:972	scanning electron microscopy	945:972	scanning electron microscopy	945:972	The optimized beads (OF) were characterized for their morphology and size by motic microscopy and scanning electron microscopy.					
33428954	1	64	theme	Alginate-Neusilin	156:172	arg1	MC					195:196	MC	195:196	MC	195:196	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
33428954	1	64	theme	Alginate-Neusilin	156:172	arg1	micro-composite					178:192	Alginate-Neusilin US2 micro-composite	156:192	The Alginate-Neusilin US2 micro-composite (MC) beads	152:203	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
33428954	9	65	theme	shelf	1283:1287	arg1	3.86 years					1328:1337	3.86 years	1328:1337	3.86 years suggested better stability after fabrication	1328:1382	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	9	65	theme	shelf	1283:1287	arg1	life					1289:1292	The estimated shelf life	1269:1292	The estimated shelf life of OF formulation	1269:1310	The estimated shelf life of OF formulation was found to be 3.86 years suggested better stability after fabrication.					
33428954	7	66	theme	gastric	1037:1043	arg1	fluids					1060:1065	simulated gastric and intestinal fluids	1027:1065	simulated gastric and intestinal fluids	1027:1065	In vitro release, kinetic studies were performed in simulated gastric and intestinal fluids.					
33428954	8	67	from	degradation	1234:1244	arg1	stomach					1260:1266	the stomach	1256:1266	the stomach	1256:1266	In vivo pharmacokinetic studies revealed better absorption of HES from optimized beads (OF) compared to HES suspension which could be due to the prevention of acidic degradation of HES in the stomach.					
33428954	0	68	theme	statistical	16:26	arg1	optimization					28:39	statistical optimization	16:39	statistical optimization of alginate-Neusilin US2	16:64	Development and statistical optimization of alginate-Neusilin US2 micro-composite beads to elicit gastric stability and sustained action of hesperidin.					
33428954	1	69	theme	US2	174:176	arg1	MC					195:196	MC	195:196	MC	195:196	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
33428954	1	69	theme	US2	174:176	arg1	micro-composite					178:192	Alginate-Neusilin US2 micro-composite	156:192	The Alginate-Neusilin US2 micro-composite (MC) beads	152:203	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
33428954	6	70	theme	optimized	851:859	arg1	beads					861:865	The optimized beads	847:865	The optimized beads	847:865	The optimized beads (OF) were characterized for their morphology and size by motic microscopy and scanning electron microscopy.					
33428954	1	71	theme	hesperidin	256:265	arg1	delivery					244:251	oral delivery	239:251	oral delivery of hesperidin (HES)	239:271	The Alginate-Neusilin US2 micro-composite (MC) beads were fabricated and optimized for oral delivery of hesperidin (HES).					
32829815	8	0	from	CNC-g-PBAT	1373:1382	arg1	contribution					1345:1356	a synergistic contribution	1331:1356	a synergistic contribution from PBATf and CNC-g-PBAT	1331:1382	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	4	1	theme	CNC-g-PBAT	678:687	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	1	theme	CNC-g-PBAT	678:687	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	6	2	theme	tensile	931:937	arg1	strength					939:946	tensile strength	931:946	tensile strength by 27 %	931:954	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	0	3	theme	butylene	64:71	arg1	poly					59:62	biodegradable poly	45:62	biodegradable poly(butylene adipate-co-terephthalate)	45:97	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	0	3	theme	butylene	64:71	arg1	adipate-co-terephthalate					73:96	butylene adipate-co-terephthalate	64:96	butylene adipate-co-terephthalate	64:96	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	1	4	from	use	303:305	arg1	agriculture					324:334	agriculture	324:334	agriculture	324:334	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	1	4	from	use	303:305	arg1	packaging					310:318	packaging	310:318	packaging	310:318	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	7	5	theme	biodegradable	1118:1130	arg1	nanocomposites					1140:1153	similar biodegradable polymer nanocomposites	1110:1153	similar biodegradable polymer nanocomposites reported in the literature	1110:1180	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	7	6	theme	reinforcement	1064:1076	arg1	efficiency					1078:1087	such reinforcement efficiency	1059:1087	such reinforcement efficiency	1059:1087	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	7	6	theme	reinforcement	1064:1076	arg1	highest					1096:1102	highest	1096:1102	highest	1096:1102	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	4	7	dep	inclusion	761:769	arg1	reinforce					783:791	reinforce	783:791	directly to reinforce a commercial PBAT	771:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	9	8	theme	matrix	1579:1584	arg1	crystallinity					1550:1562	the crystallinity	1546:1562	the crystallinity of the polymer matrix	1546:1584	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	8	9	theme	wide-angle	1236:1245	arg1	diffraction					1253:1263	wide-angle X-ray diffraction	1236:1263	wide-angle X-ray diffraction	1236:1263	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	10	10	theme	polymer	1732:1738	arg1	films					1754:1758	high-performance biodegradable polymer nanocomposite films	1701:1758	high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion	1701:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	9	11	theme	composites	1462:1471	arg1	toughness					1445:1453	toughness	1445:1453	toughness	1445:1453	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	9	11	theme	composites	1462:1471	arg1	stiffness					1431:1439	stiffness	1431:1439	stiffness	1431:1439	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	7	12	theme	similar	1110:1116	arg1	nanocomposites					1140:1153	similar biodegradable polymer nanocomposites	1110:1153	similar biodegradable polymer nanocomposites reported in the literature	1110:1180	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	5	13	theme	low	832:834	arg1	usage					840:844	an exceptionally low CNC usage	815:844	an exceptionally low CNC usage of 0.02 wt	815:855	At an exceptionally low CNC usage of 0.02 wt.					
32829815	0	14	theme	low	106:108	arg1	usage					124:128	a low nanoinclusion usage	104:128	a low nanoinclusion usage	104:128	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	8	15	theme	diffraction	1253:1263	arg1	measurements					1265:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements	1183:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements	1183:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	4	16	theme	CNC	667:669	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	16	theme	CNC	667:669	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	17	theme	nanocrystal	654:664	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	17	theme	nanocrystal	654:664	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	7	18	theme	such	1059:1062	arg1	efficiency					1078:1087	such reinforcement efficiency	1059:1087	such reinforcement efficiency	1059:1087	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	7	18	theme	such	1059:1062	arg1	highest					1096:1102	highest	1096:1102	highest	1096:1102	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	2	19	from	toughness	453:461	arg1	reinforcement					496:508	the reinforcement	492:508	the reinforcement	492:508	However, a high content of nanoinclusions is usually required, leading to a tradeoff between composite toughness and strength or stiffness in the reinforcement.					
32829815	8	20	theme	X-ray	1247:1251	arg1	diffraction					1253:1263	wide-angle X-ray diffraction	1236:1263	wide-angle X-ray diffraction	1236:1263	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	4	21	theme	cellulose	644:652	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	21	theme	cellulose	644:652	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	6	22	theme	Young	906:910	arg1	modulus					914:920	the Young's modulus	902:920	the Young's modulus	902:920	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	9	23	theme	polymer	1571:1577	arg1	matrix					1579:1584	the polymer matrix	1567:1584	the polymer matrix	1567:1584	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	3	24	theme	reinforcement	542:554	arg1	strategy					556:563	an iterative reinforcement strategy	529:563	an iterative reinforcement strategy that uses one nanocomposite to reinforce PBAT	529:609	Herein, we report an iterative reinforcement strategy that uses one nanocomposite to reinforce PBAT.					
32829815	2	25	theme	high	361:364	arg1	content					366:372	a high content	359:372	a high content of nanoinclusions	359:390	However, a high content of nanoinclusions is usually required, leading to a tradeoff between composite toughness and strength or stiffness in the reinforcement.					
32829815	9	26	theme	strengthening	1602:1614	arg1	effect					1631:1636	the strengthening and toughening effect	1598:1636	the strengthening and toughening effect	1598:1636	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	6	27	from	break	971:975	arg1	strength					939:946	tensile strength	931:946	tensile strength by 27 %	931:954	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	6	27	from	break	971:975	arg1	enhancement					887:897	a simultaneous enhancement	872:897	a simultaneous enhancement of the Young's modulus by 26 %	872:928	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	6	27	from	break	971:975	arg1	elongation					957:966	elongation	957:966	elongation at break by 37 %	957:983	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	6	27	from	break	971:975	arg1	toughness					990:998	toughness	990:998	toughness	990:998	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	4	28	theme	ungrafted/free	718:731	arg1	PBATf					739:743	PBATf	739:743	PBATf	739:743	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	28	theme	ungrafted/free	718:731	arg1	PBAT					733:736	ungrafted/free PBAT	718:736	ungrafted/free PBAT (PBATf)	718:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	8	29	from	PBATf	1363:1367	arg1	contribution					1345:1356	a synergistic contribution	1331:1356	a synergistic contribution from PBATf and CNC-g-PBAT	1331:1382	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	1	30	theme	biodegradable	189:201	arg1	poly					221:224	poly	221:224	poly(butylene adipate-co-terephthalate) (PBAT)	221:266	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	1	30	theme	biodegradable	189:201	arg1	others					272:277	others	272:277	others	272:277	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	1	30	theme	biodegradable	189:201	arg1	polymers					203:210	biodegradable polymers	189:210	biodegradable polymers	189:210	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	8	31	theme	rheology	1187:1194	arg1	measurements					1265:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements	1183:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements	1183:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	10	32	theme	nanocomposite	1740:1752	arg1	films					1754:1758	high-performance biodegradable polymer nanocomposite films	1701:1758	high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion	1701:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	8	33	theme	calorimetry	1219:1229	arg1	measurements					1265:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements	1183:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements	1183:1276	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	1	34	theme	polymers	203:210	arg1	strengthening					156:168	strengthening	156:168	strengthening	156:168	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	1	34	theme	polymers	203:210	arg1	toughening					175:184	toughening	175:184	toughening	175:184	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	1	34	theme	polymers	203:210	arg1	stiffening					144:153	Simultaneous stiffening	131:153	Simultaneous stiffening	131:153	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	10	35	theme	biodegradable	1718:1730	arg1	films					1754:1758	high-performance biodegradable polymer nanocomposite films	1701:1758	high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion	1701:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	0	36	theme	nanoinclusion	110:122	arg1	usage					124:128	a low nanoinclusion usage	104:128	a low nanoinclusion usage	104:128	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	2	37	from	strength	467:474	arg1	reinforcement					496:508	the reinforcement	492:508	the reinforcement	492:508	However, a high content of nanoinclusions is usually required, leading to a tradeoff between composite toughness and strength or stiffness in the reinforcement.					
32829815	3	38	theme	iterative	532:540	arg1	strategy					556:563	an iterative reinforcement strategy	529:563	an iterative reinforcement strategy that uses one nanocomposite to reinforce PBAT	529:609	Herein, we report an iterative reinforcement strategy that uses one nanocomposite to reinforce PBAT.					
32829815	7	39	theme	polymer	1132:1138	arg1	nanocomposites					1140:1153	similar biodegradable polymer nanocomposites	1110:1153	similar biodegradable polymer nanocomposites reported in the literature	1110:1180	To the best of our knowledge, such reinforcement efficiency is the highest among similar biodegradable polymer nanocomposites reported in the literature.					
32829815	1	40	dep	packaging	310:318	arg1	applications					336:347	applications	336:347	applications	336:347	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	10	41	theme	low	1817:1819	arg1	nanoinclusion					1831:1843	nanoinclusion	1831:1843	nanoinclusion	1831:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	10	41	theme	low	1817:1819	arg1	amount					1821:1826	a very low amount	1810:1826	a very low amount of nanoinclusion	1810:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	4	42	theme	polymerized	632:642	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	42	theme	polymerized	632:642	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	2	43	theme	composite	443:451	arg1	toughness					453:461	composite toughness	443:461	composite toughness	443:461	However, a high content of nanoinclusions is usually required, leading to a tradeoff between composite toughness and strength or stiffness in the reinforcement.					
32829815	2	44	from	stiffness	479:487	arg1	reinforcement					496:508	the reinforcement	492:508	the reinforcement	492:508	However, a high content of nanoinclusions is usually required, leading to a tradeoff between composite toughness and strength or stiffness in the reinforcement.					
32829815	9	45	theme	polymer	1517:1523	arg1	matrix					1525:1530	the polymer matrix	1513:1530	the polymer matrix	1513:1530	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	2	46	theme	nanoinclusions	377:390	arg1	content					366:372	a high content	359:372	a high content of nanoinclusions	359:390	However, a high content of nanoinclusions is usually required, leading to a tradeoff between composite toughness and strength or stiffness in the reinforcement.					
32829815	0	47	theme	biodegradable	45:57	arg1	poly					59:62	biodegradable poly	45:62	biodegradable poly(butylene adipate-co-terephthalate)	45:97	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	0	47	theme	biodegradable	45:57	arg1	adipate-co-terephthalate					73:96	butylene adipate-co-terephthalate	64:96	butylene adipate-co-terephthalate	64:96	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	0	48	with	strengthening	12:24	arg1	usage					124:128	a low nanoinclusion usage	104:128	a low nanoinclusion usage	104:128	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	8	49	theme	differential	1197:1208	arg1	calorimetry					1219:1229	differential scanning calorimetry	1197:1229	differential scanning calorimetry	1197:1229	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	4	50	theme	commercial	795:804	arg1	PBAT					806:809	a commercial PBAT	793:809	a commercial PBAT	793:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	9	51	theme	PBATf	1411:1415	arg1	use					1404:1406	the use	1400:1406	the use of PBATf	1400:1415	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
32829815	4	52	used	used	750:753	arg2	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	52	used	used	750:753	arg2	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	6	53	theme	simultaneous	874:885	arg1	enhancement					887:897	a simultaneous enhancement	872:897	a simultaneous enhancement of the Young's modulus by 26 %	872:928	%, we achieved a simultaneous enhancement of the Young's modulus by 26 %, tensile strength by 27 %, elongation at break by 37 %, and toughness by 56 % over those for PBAT.					
32829815	0	54	with	Stiffening	0:9	arg1	usage					124:128	a low nanoinclusion usage	104:128	a low nanoinclusion usage	104:128	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	8	55	theme	mechanical	1292:1301	arg1	reinforcement					1303:1315	the mechanical reinforcement	1288:1315	the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT	1288:1382	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	10	56	theme	nanoinclusion	1831:1843	arg1	nanoinclusion					1831:1843	nanoinclusion	1831:1843	nanoinclusion	1831:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	10	56	theme	nanoinclusion	1831:1843	arg1	amount					1821:1826	a very low amount	1810:1826	a very low amount of nanoinclusion	1810:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	8	57	theme	scanning	1210:1217	arg1	calorimetry					1219:1229	differential scanning calorimetry	1197:1229	differential scanning calorimetry	1197:1229	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	1	58	theme	Simultaneous	131:142	arg1	stiffening					144:153	Simultaneous stiffening	131:153	Simultaneous stiffening	131:153	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	10	59	theme	high-performance	1701:1716	arg1	films					1754:1758	high-performance biodegradable polymer nanocomposite films	1701:1758	high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion	1701:1843	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	8	60	theme	synergistic	1333:1343	arg1	contribution					1345:1356	a synergistic contribution	1331:1356	a synergistic contribution from PBATf and CNC-g-PBAT	1331:1382	The rheology, differential scanning calorimetry, and wide-angle X-ray diffraction measurements confirmed the mechanical reinforcement attributed to a synergistic contribution from PBATf and CNC-g-PBAT.					
32829815	4	61	theme	grafting	623:630	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	61	theme	grafting	623:630	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	10	62	theme	agricultural	1778:1789	arg1	applications					1791:1802	agricultural applications	1778:1802	agricultural applications	1778:1802	The strategy proposed here is greatly beneficial to producing high-performance biodegradable polymer nanocomposite films for packaging and agricultural applications using a very low amount of nanoinclusion.					
32829815	4	63	theme	in-situ	615:621	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	63	theme	in-situ	615:621	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	64	theme	/PBAT	671:675	arg1	nanocomposite					690:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite	612:702	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf)	612:744	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	4	64	theme	/PBAT	671:675	arg1	inclusion					761:769	an inclusion	758:769	an inclusion directly to reinforce a commercial PBAT	758:809	An in-situ grafting polymerized cellulose nanocrystal (CNC)/PBAT (CNC-g-PBAT) nanocomposite consisting of ungrafted/free PBAT (PBATf) was used as an inclusion directly to reinforce a commercial PBAT.					
32829815	5	65	theme	CNC	836:838	arg1	usage					840:844	an exceptionally low CNC usage	815:844	an exceptionally low CNC usage of 0.02 wt	815:855	At an exceptionally low CNC usage of 0.02 wt.					
32829815	1	66	theme	butylene	226:233	arg1	poly					221:224	poly	221:224	poly(butylene adipate-co-terephthalate) (PBAT)	221:266	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	1	66	theme	butylene	226:233	arg1	adipate-co-terephthalate					235:258	butylene adipate-co-terephthalate	226:258	butylene adipate-co-terephthalate	226:258	Simultaneous stiffening, strengthening, and toughening of biodegradable polymers, such as poly(butylene adipate-co-terephthalate) (PBAT) and others, is necessary for their use in packaging and agriculture applications.					
32829815	0	67	theme	poly	59:62	arg1	strengthening					12:24	strengthening	12:24	strengthening	12:24	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	0	67	theme	poly	59:62	arg1	toughening					31:40	toughening	31:40	toughening	31:40	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	0	67	theme	poly	59:62	arg1	Stiffening					0:9	Stiffening	0:9	Stiffening	0:9	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	0	68	with	toughening	31:40	arg1	usage					124:128	a low nanoinclusion usage	104:128	a low nanoinclusion usage	104:128	Stiffening, strengthening, and toughening of biodegradable poly(butylene adipate-co-terephthalate) with a low nanoinclusion usage.					
32829815	5	69	theme	wt	854:855	arg1	usage					840:844	an exceptionally low CNC usage	815:844	an exceptionally low CNC usage of 0.02 wt	815:855	At an exceptionally low CNC usage of 0.02 wt.					
32829815	9	70	theme	toughening	1620:1629	arg1	effect					1631:1636	the strengthening and toughening effect	1598:1636	the strengthening and toughening effect	1598:1636	In particular, the use of PBATf enhanced both stiffness and toughness of the composites, while the CNC-g-PBAT interacted within the polymer matrix and increased the crystallinity of the polymer matrix, leading to the strengthening and toughening effect.					
33590754	0	0	theme	Nanofibril	120:129	arg1	Aerogels					141:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	6	1	from	utilization	1171:1181	arg1	fields					1232:1237	high-tech fields	1222:1237	high-tech fields	1222:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	1	2	theme	renewable	206:214	arg1	cellulose					239:247	cellulose	239:247	cellulose	239:247	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	1	2	theme	renewable	206:214	arg1	biomaterials					216:227	the renewable biomaterials	202:227	the renewable biomaterials including cellulose	202:247	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	1	3	theme	pressure	304:311	arg1	sensors					313:319	pressure sensors	304:319	pressure sensors	304:319	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	0	4	theme	Cellulose	110:118	arg1	Aerogels					141:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	5	5	theme	footstep	972:979	arg1	recognition					981:991	footstep recognition	972:991	footstep recognition	972:991	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	2	6	with	nanocellulose	355:367	arg1	nanofibrils					424:434	wood-derived cellulose nanofibrils	401:434	wood-derived cellulose nanofibrils	401:434	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	2	6	with	nanocellulose	355:367	arg1	conductivity					384:395	excellent conductivity	374:395	excellent conductivity	374:395	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	3	7	theme	resistive-type	663:676	arg1	sensor					687:692	the resistive-type pressure sensor	659:692	the resistive-type pressure sensor on a paper substrate	659:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	2	8	theme	bacterial	345:353	arg1	nanocellulose					355:367	the carbonized bacterial nanocellulose	330:367	the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils	330:434	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	6	9	theme	high-tech	1222:1230	arg1	fields					1232:1237	high-tech fields	1222:1237	high-tech fields	1222:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	0	10	theme	Composite	131:139	arg1	Aerogels					141:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	3	11	theme	active	635:640	arg1	aerogel					549:555	The obtained composite aerogel	526:555	The obtained composite aerogel	526:555	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	11	theme	active	635:640	arg1	layer					642:646	an active layer	632:646	an active layer to prepare the resistive-type pressure sensor on a paper substrate	632:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	2	12	theme	carbonized	334:343	arg1	nanocellulose					355:367	the carbonized bacterial nanocellulose	330:367	the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils	330:434	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	6	13	theme	sustainable	1186:1196	arg1	biomaterials					1206:1217	sustainable natural biomaterials	1186:1217	sustainable natural biomaterials in high-tech fields	1186:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	6	14	theme	sensors	1155:1161	arg1	utilization					1171:1181	the utilization	1167:1181	the utilization of sustainable natural biomaterials in high-tech fields	1167:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	6	14	theme	sensors	1155:1161	arg1	development					1125:1135	the development	1121:1135	the development of green pressure sensors	1121:1161	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	6	15	theme	green	1140:1144	arg1	sensors					1155:1161	green pressure sensors	1140:1161	green pressure sensors	1140:1161	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	2	16	theme	directional	480:490	arg1	ice-templating					492:505	directional ice-templating	480:505	directional ice-templating	480:505	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	5	17	with	communication	998:1010	arg1	smartphones					1017:1027	smartphones	1017:1027	smartphones	1017:1027	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	4	18	theme	remarkable	746:755	arg1	flexibility					757:767	remarkable flexibility	746:767	remarkable flexibility	746:767	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	5	19	with	recognition	981:991	arg1	smartphones					1017:1027	smartphones	1017:1027	smartphones	1017:1027	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	4	20	theme	fast	770:773	arg1	response					775:782	fast response	770:782	fast response	770:782	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	0	21	theme	Flexible	0:7	arg1	Sensors					45:51	Flexible and Sensitivity-Adjustable Pressure Sensors	0:51	Flexible and Sensitivity-Adjustable Pressure Sensors	0:51	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	6	22	from	development	1125:1135	arg1	fields					1232:1237	high-tech fields	1222:1237	high-tech fields	1222:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	2	23	theme	excellent	374:382	arg1	conductivity					384:395	excellent conductivity	374:395	excellent conductivity	374:395	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	6	24	from	fields	1232:1237	arg1	utilization					1171:1181	the utilization	1167:1181	the utilization of sustainable natural biomaterials in high-tech fields	1167:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	6	24	from	fields	1232:1237	arg1	development					1125:1135	the development	1121:1135	the development of green pressure sensors	1121:1161	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	3	25	theme	obtained	530:537	arg1	aerogel					549:555	The obtained composite aerogel	526:555	The obtained composite aerogel	526:555	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	25	theme	obtained	530:537	arg1	layer					642:646	an active layer	632:646	an active layer to prepare the resistive-type pressure sensor on a paper substrate	632:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	0	26	theme	Pressure	36:43	arg1	Sensors					45:51	Flexible and Sensitivity-Adjustable Pressure Sensors	0:51	Flexible and Sensitivity-Adjustable Pressure Sensors	0:51	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	4	27	theme	pressure	846:853	arg1	range					855:859	a wide pressure range	839:859	a wide pressure range (0-100 kPa)	839:871	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	4	27	theme	pressure	846:853	arg1	kPa					868:870	0-100 kPa	862:870	0-100 kPa	862:870	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	4	28	theme	pressure	721:728	arg1	sensor					730:735	This pressure sensor	716:735	This pressure sensor	716:735	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	0	29	theme	Sensitivity-Adjustable	13:34	arg1	Sensors					45:51	Flexible and Sensitivity-Adjustable Pressure Sensors	0:51	Flexible and Sensitivity-Adjustable Pressure Sensors	0:51	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	4	30	theme	wide	841:844	arg1	range					855:859	a wide pressure range	839:859	a wide pressure range (0-100 kPa)	839:871	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	4	30	theme	wide	841:844	arg1	kPa					868:870	0-100 kPa	862:870	0-100 kPa	862:870	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	3	31	from	sensor	687:692	arg1	substrate					705:713	a paper substrate	697:713	a paper substrate	697:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	32	theme	aligned	591:597	arg1	channels					599:606	aligned channels	591:606	aligned channels	591:606	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	6	33	theme	biomaterials	1206:1217	arg1	utilization					1171:1181	the utilization	1167:1181	the utilization of sustainable natural biomaterials in high-tech fields	1167:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	6	33	theme	biomaterials	1206:1217	arg1	development					1125:1135	the development	1121:1135	the development of green pressure sensors	1121:1161	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	3	34	theme	composite	539:547	arg1	aerogel					549:555	The obtained composite aerogel	526:555	The obtained composite aerogel	526:555	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	34	theme	composite	539:547	arg1	layer					642:646	an active layer	632:646	an active layer to prepare the resistive-type pressure sensor on a paper substrate	632:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	1	35	theme	flexible	274:281	arg1	electronics					283:293	flexible electronics	274:293	flexible electronics	274:293	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	0	36	theme	Carbonized	62:71	arg1	Aerogels					141:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	3	37	contain	has	564:566	arg1	layer					642:646	an active layer	632:646	an active layer to prepare the resistive-type pressure sensor on a paper substrate	632:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	37	contain	has	564:566	arg1	aerogel					549:555	The obtained composite aerogel	526:555	The obtained composite aerogel	526:555	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	37	contain	has	564:566	arg2	channels					599:606	aligned channels	591:606	aligned channels	591:606	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	37	contain	has	564:566	arg2	structure					577:585	a porous structure	568:585	a porous structure	568:585	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	1	38	used	used	266:269	arg2	biomaterials					216:227	the renewable biomaterials	202:227	the renewable biomaterials including cellulose	202:247	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	1	38	used	used	266:269	arg2	cellulose					239:247	cellulose	239:247	cellulose	239:247	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	6	39	theme	natural	1198:1204	arg1	biomaterials					1206:1217	sustainable natural biomaterials	1186:1217	sustainable natural biomaterials in high-tech fields	1186:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	3	40	theme	porous	570:575	arg1	structure					577:585	a porous structure	568:585	a porous structure	568:585	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	3	41	theme	pressure	678:685	arg1	sensor					687:692	the resistive-type pressure sensor	659:692	the resistive-type pressure sensor on a paper substrate	659:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
33590754	2	42	theme	cellulose	414:422	arg1	nanofibrils					424:434	wood-derived cellulose nanofibrils	401:434	wood-derived cellulose nanofibrils	401:434	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	6	43	from	biomaterials	1206:1217	arg1	fields					1232:1237	high-tech fields	1222:1237	high-tech fields	1222:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	1	44	theme	environmental	174:186	arg1	friendliness					188:199	environmental friendliness	174:199	environmental friendliness	174:199	For sustainability and environmental friendliness, the renewable biomaterials including cellulose have been widely used in flexible electronics, such as pressure sensors.					
33590754	6	45	theme	novel	1101:1105	arg1	insights					1107:1114	novel insights	1101:1114	novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields	1101:1237	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	4	46	theme	adjustable	813:822	arg1	sensitivity					824:834	especially adjustable sensitivity	802:834	especially adjustable sensitivity	802:834	This pressure sensor exhibits remarkable flexibility, fast response, reliability, and especially adjustable sensitivity in a wide pressure range (0-100 kPa).					
33590754	2	47	theme	wood-derived	401:412	arg1	nanofibrils					424:434	wood-derived cellulose nanofibrils	401:434	wood-derived cellulose nanofibrils	401:434	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	0	48	theme	Nanocellulose/Wood-Derived	83:108	arg1	Aerogels					141:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	5	49	theme	potential	922:930	arg1	recognition					981:991	footstep recognition	972:991	footstep recognition	972:991	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	5	49	theme	potential	922:930	arg1	detection					961:969	motion detection	954:969	motion detection	954:969	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	5	49	theme	potential	922:930	arg1	applications					932:943	potential applications	922:943	potential applications	922:943	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	5	49	theme	potential	922:930	arg1	communication					998:1010	communication	998:1010	communication with smartphones via Bluetooth	998:1041	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	5	50	with	detection	961:969	arg1	smartphones					1017:1027	smartphones	1017:1027	smartphones	1017:1027	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	5	51	theme	working	900:906	arg1	mechanism					908:916	the sensor's working mechanism	887:916	the sensor's working mechanism	887:916	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	5	52	theme	motion	954:959	arg1	detection					961:969	motion detection	954:969	motion detection	954:969	In addition, the sensor's working mechanism and potential applications, such as motion detection, footstep recognition, and communication with smartphones via Bluetooth, are also well demonstrated.					
33590754	6	53	theme	pressure	1146:1153	arg1	sensors					1155:1161	green pressure sensors	1140:1161	green pressure sensors	1140:1161	Moreover, this work provides novel insights into the development of green pressure sensors and the utilization of sustainable natural biomaterials in high-tech fields.					
33590754	0	54	theme	Bacterial	73:81	arg1	Aerogels					141:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels	62:148	Flexible and Sensitivity-Adjustable Pressure Sensors Based on Carbonized Bacterial Nanocellulose/Wood-Derived Cellulose Nanofibril Composite Aerogels.					
33590754	2	55	link	wood-derived	401:412	arg1	nanofibrils					424:434	wood-derived cellulose nanofibrils	401:434	wood-derived cellulose nanofibrils	401:434	Herein, the carbonized bacterial nanocellulose with excellent conductivity and wood-derived cellulose nanofibrils are combined to prepare the aerogel through directional ice-templating and freeze-drying.					
33590754	3	56	theme	paper	699:703	arg1	substrate					705:713	a paper substrate	697:713	a paper substrate	697:713	The obtained composite aerogel, which has a porous structure and aligned channels, is further employed as an active layer to prepare the resistive-type pressure sensor on a paper substrate.					
32958727	5	0	dep	group	752:756	arg1	group					875:879	-treated group	866:879	-treated group	866:879	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	8	1	theme	more	1138:1141	arg1	Bacteroides					1143:1153	more Bacteroides	1138:1153	more Bacteroides in ETBF/AOM/DSS + Z (60) group	1138:1184	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	2	2	theme	inflammatory	262:273	arg1	diseases					281:288	inflammatory bowel diseases	262:288	inflammatory bowel diseases (IBD)	262:294	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	2	2	theme	inflammatory	262:273	arg1	IBD					291:293	IBD	291:293	IBD	291:293	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	5	3	theme	ETBF/AOM/	810:818	arg1	DSS					820:822	ETBF/AOM/ DSS	810:822	ETBF/AOM/ DSS	810:822	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	7	4	theme	Microbiota	989:998	arg1	compositions					1000:1011	Microbiota compositions	989:1011	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone	989:1095	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	9	5	theme	linear	1328:1333	arg1	analysis					1348:1355	linear discriminant analysis	1328:1355	linear discriminant analysis of effect size (LEfSe) analysis	1328:1387	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	12	6	theme	intestinal	1806:1815	arg1	microbiota					1817:1826	intestinal microbiota	1806:1826	intestinal microbiota	1806:1826	In this work, we demonstrate that zerumbone could restore the composition of intestinal microbiota.					
32958727	10	7	theme	given	1486:1490	arg1	zerumbone					1492:1500	given zerumbone	1486:1500	given zerumbone	1486:1500	However, after given zerumbone, the taxonomic relative abundance was increased.					
32958727	4	8	with	compound	562:569	arg1	effect					591:596	anti-bacterial effect	576:596	anti-bacterial effect	576:596	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	5	9	theme	AOM/DSS	774:780	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	9	theme	AOM/DSS	774:780	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	2	10	theme	gut	206:208	arg1	microbiota					210:219	gut microbiota	206:219	gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer	206:311	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	9	11	theme	effect	1360:1365	arg1	size					1367:1370	effect size	1360:1370	effect size (LEfSe) analysis	1360:1387	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	9	11	theme	effect	1360:1365	arg1	LEfSe					1373:1377	LEfSe	1373:1377	LEfSe	1373:1377	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	9	12	theme	size	1367:1370	arg1	analysis					1380:1387	effect size (LEfSe) analysis	1360:1387	effect size (LEfSe) analysis	1360:1387	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	4	13	theme	intestinal	644:653	arg1	composition					666:676	intestinal microbiota composition	644:676	intestinal microbiota composition	644:676	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	0	14	theme	AOM/DSS	64:70	arg1	Mice					72:75	ETBF Colonized AOM/DSS Mice	49:75	ETBF Colonized AOM/DSS Mice	49:75	Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice.					
32958727	0	15	theme	Gut	19:21	arg1	Composition					34:44	Gut Microbiota Composition	19:44	Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice	19:75	Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice.					
32958727	1	16	theme	common	126:131	arg1	worldwide					152:160	common malignant neoplasm worldwide	126:160	common malignant neoplasm worldwide	126:160	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	6	17	theme	dye	906:908	arg1	analysis					928:935	reversible dye terminators-based analysis	895:935	reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison	895:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	4	18	theme	ETBF	471:474	arg1	mice					486:489	ETBF colonized mice	471:489	ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect,	471:597	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	8	19	theme	colonized	1205:1213	arg1	group					1223:1227	ETBF colonized AOM/DSS group	1200:1227	ETBF colonized AOM/DSS group	1200:1227	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	5	20	theme	ETBF	729:732	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	20	theme	ETBF	729:732	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	1	21	theme	neoplasm	143:150	arg1	worldwide					152:160	common malignant neoplasm worldwide	126:160	common malignant neoplasm worldwide	126:160	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	3	22	theme	representative	330:343	arg1	bacteria					345:352	the most representative bacteria	321:352	the most representative bacteria involved in CRC	321:368	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	9	23	theme	colonized	1446:1454	arg1	group					1464:1468	the ETBF colonized AOM/DSS group	1437:1468	the ETBF colonized AOM/DSS group	1437:1468	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	5	24	theme	mice	707:710	arg1	mice					707:710	mice	707:710	mice	707:710	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	24	theme	mice	707:710	arg1	groups					697:702	Four experimental groups	679:702	Four experimental groups of mice	679:710	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	10	25	theme	relative	1517:1524	arg1	abundance					1526:1534	the taxonomic relative abundance	1503:1534	the taxonomic relative abundance	1503:1534	However, after given zerumbone, the taxonomic relative abundance was increased.					
32958727	5	26	theme	colonized	734:742	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	26	theme	colonized	734:742	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	1	27	theme	Colorectal	78:87	arg1	cause					117:121	the leading cause	105:121	the leading cause of common malignant neoplasm worldwide	105:160	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	1	27	theme	Colorectal	78:87	arg1	CRC					97:99	CRC	97:99	CRC	97:99	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	1	27	theme	Colorectal	78:87	arg1	cancer					89:94	Colorectal cancer	78:94	Colorectal cancer (CRC)	78:100	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	6	28	theme	group	971:975	arg1	comparison					977:986	group comparison	971:986	group comparison	971:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	0	29	from	Composition	34:44	arg1	Mice					72:75	ETBF Colonized AOM/DSS Mice	49:75	ETBF Colonized AOM/DSS Mice	49:75	Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice.					
32958727	5	30	theme	colonized	764:772	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	30	theme	colonized	764:772	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	6	31	theme	V3-V4	961:965	arg1	analysis					928:935	reversible dye terminators-based analysis	895:935	reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison	895:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	4	32	theme	/dextran	514:521	arg1	DSS					540:542	DSS	540:542	DSS	540:542	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	4	32	theme	/dextran	514:521	arg1	sodium					532:537	azoxymethane (AOM)/dextran sulphate sodium	496:537	azoxymethane (AOM)/dextran sulphate sodium (DSS)	496:543	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	7	33	theme	colonized	1053:1061	arg1	group					1071:1075	ETBF colonized AOM/DSS group	1048:1075	ETBF colonized AOM/DSS group	1048:1075	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	5	34	theme	mg	858:859	arg1	zerumbone					844:852	only zerumbone	839:852	only zerumbone (60 mg kg-1)	839:865	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	34	theme	mg	858:859	arg1	kg-1					861:864	60 mg kg-1	855:864	60 mg kg-1	855:864	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	6	35	theme	gene	949:952	arg1	V3-V4					961:965	16S rRNA gene region V3-V4	940:965	16S rRNA gene region V3-V4 for group comparison	940:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	2	36	theme	Many	163:166	arg1	studies					168:174	Many studies	163:174	Many studies	163:174	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	4	37	used	used	466:469	arg2	We					463:464	We	463:464	We	463:464	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	5	38	theme	AOM/DSS	744:750	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	38	theme	AOM/DSS	744:750	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	6	39	theme	16S	940:942	arg1	rRNA					944:947	16S rRNA	940:947	16S rRNA gene region V3-V4 for group comparison	940:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	3	40	theme	enterotoxigenic	373:387	arg1	ETBF					411:414	ETBF	411:414	ETBF	411:414	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	3	40	theme	enterotoxigenic	373:387	arg1	fragilis					401:408	enterotoxigenic Bacteroides fragilis	373:408	enterotoxigenic Bacteroides fragilis (ETBF)	373:415	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	3	40	theme	enterotoxigenic	373:387	arg1	species					420:426	a species	418:426	a species belonging to phylum Bacteroidetes	418:460	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	4	41	theme	azoxymethane	496:507	arg1	DSS					540:542	DSS	540:542	DSS	540:542	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	4	41	theme	azoxymethane	496:507	arg1	sodium					532:537	azoxymethane (AOM)/dextran sulphate sodium	496:537	azoxymethane (AOM)/dextran sulphate sodium (DSS)	496:543	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	5	42	theme	sham	723:726	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	42	theme	sham	723:726	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	11	43	theme	composition	1716:1726	arg1	balance					1691:1697	the balance	1687:1697	the balance of gut microbial composition	1687:1726	These findings suggest that zerumbone not only influenced the microbial diversity and richness, but also could be helpful for enhancing the balance of gut microbial composition.					
32958727	0	44	theme	ETBF	49:52	arg1	Mice					72:75	ETBF Colonized AOM/DSS Mice	49:75	ETBF Colonized AOM/DSS Mice	49:75	Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice.					
32958727	2	45	theme	various	237:243	arg1	diseases					281:288	inflammatory bowel diseases	262:288	inflammatory bowel diseases (IBD)	262:294	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	2	45	theme	various	237:243	arg1	diseases					245:252	various diseases	237:252	various diseases such as inflammatory bowel diseases (IBD) and colon cancer	237:311	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	2	45	theme	various	237:243	arg1	cancer					306:311	colon cancer	300:311	colon cancer	300:311	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	11	46	theme	gut	1702:1704	arg1	composition					1716:1726	gut microbial composition	1702:1726	gut microbial composition	1702:1726	These findings suggest that zerumbone not only influenced the microbial diversity and richness, but also could be helpful for enhancing the balance of gut microbial composition.					
32958727	5	47	theme	group	782:786	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	47	theme	group	782:786	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	7	48	theme	Z	1031:1031	arg1	group					1038:1042	ETBF/AOM/DSS + Z (60) group	1016:1042	ETBF/AOM/DSS + Z (60) group	1016:1042	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	7	49	theme	ETBF/AOM/DSS	1016:1027	arg1	group					1038:1042	ETBF/AOM/DSS + Z (60) group	1016:1042	ETBF/AOM/DSS + Z (60) group	1016:1042	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	9	50	theme	analysis	1380:1387	arg1	analysis					1348:1355	linear discriminant analysis	1328:1355	linear discriminant analysis of effect size (LEfSe) analysis	1328:1387	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	2	51	theme	bowel	275:279	arg1	diseases					281:288	inflammatory bowel diseases	262:288	inflammatory bowel diseases (IBD)	262:294	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	2	51	theme	bowel	275:279	arg1	IBD					291:293	IBD	291:293	IBD	291:293	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	1	52	theme	worldwide	152:160	arg1	cause					117:121	the leading cause	105:121	the leading cause of common malignant neoplasm worldwide	105:160	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	1	52	theme	worldwide	152:160	arg1	cancer					89:94	Colorectal cancer	78:94	Colorectal cancer (CRC)	78:100	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	2	53	theme	colon	300:304	arg1	cancer					306:311	colon cancer	300:311	colon cancer	300:311	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	5	54	theme	zerumbone	788:796	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	54	theme	zerumbone	788:796	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	8	55	theme	ETBF	1200:1203	arg1	group					1223:1227	ETBF colonized AOM/DSS group	1200:1227	ETBF colonized AOM/DSS group	1200:1227	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	4	56	theme	anti-bacterial	576:589	arg1	effect					591:596	anti-bacterial effect	576:596	anti-bacterial effect	576:596	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	5	57	theme	experimental	684:695	arg1	mice					707:710	mice	707:710	mice	707:710	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	57	theme	experimental	684:695	arg1	groups					697:702	Four experimental groups	679:702	Four experimental groups of mice	679:710	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	9	58	theme	discriminant	1335:1346	arg1	analysis					1348:1355	linear discriminant analysis	1328:1355	linear discriminant analysis of effect size (LEfSe) analysis	1328:1387	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	8	59	theme	Z	1173:1173	arg1	group					1180:1184	ETBF/AOM/DSS + Z (60) group	1158:1184	ETBF/AOM/DSS + Z (60) group	1158:1184	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	2	60	theme	microbiota	210:219	arg1	compositions					190:201	compositions	190:201	compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer	190:311	Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer.					
32958727	8	61	from	Bacteroides	1143:1153	arg1	group					1180:1184	ETBF/AOM/DSS + Z (60) group	1158:1184	ETBF/AOM/DSS + Z (60) group	1158:1184	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	12	62	theme	microbiota	1817:1826	arg1	composition					1791:1801	the composition	1787:1801	the composition of intestinal microbiota	1787:1826	In this work, we demonstrate that zerumbone could restore the composition of intestinal microbiota.					
32958727	5	63	dep	used	717:720	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	63	dep	used	717:720	arg1	zerumbone					844:852	only zerumbone	839:852	only zerumbone (60 mg kg-1)	839:865	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	63	dep	used	717:720	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	63	dep	used	717:720	arg1	kg-1					861:864	60 mg kg-1	855:864	60 mg kg-1	855:864	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	7	64	theme	ETBF	1048:1051	arg1	group					1071:1075	ETBF colonized AOM/DSS group	1048:1075	ETBF colonized AOM/DSS group	1048:1075	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	1	65	theme	leading	109:115	arg1	cause					117:121	the leading cause	105:121	the leading cause of common malignant neoplasm worldwide	105:160	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	1	65	theme	leading	109:115	arg1	cancer					89:94	Colorectal cancer	78:94	Colorectal cancer (CRC)	78:100	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	6	66	theme	terminators-based	910:926	arg1	analysis					928:935	reversible dye terminators-based analysis	895:935	reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison	895:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	4	67	theme	colonized	476:484	arg1	mice					486:489	ETBF colonized mice	471:489	ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect,	471:597	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	11	68	theme	microbial	1613:1621	arg1	diversity					1623:1631	the microbial diversity	1609:1631	the microbial diversity	1609:1631	These findings suggest that zerumbone not only influenced the microbial diversity and richness, but also could be helpful for enhancing the balance of gut microbial composition.					
32958727	5	69	used	used	717:720	arg2	groups					697:702	Four experimental groups	679:702	Four experimental groups of mice	679:710	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	69	used	used	717:720	arg2	mice					707:710	mice	707:710	mice	707:710	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	1	70	theme	malignant	133:141	arg1	worldwide					152:160	common malignant neoplasm worldwide	126:160	common malignant neoplasm worldwide	126:160	Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide.					
32958727	6	71	theme	reversible	895:904	arg1	analysis					928:935	reversible dye terminators-based analysis	895:935	reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison	895:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	4	72	theme	microbiota	655:664	arg1	composition					666:676	intestinal microbiota composition	644:676	intestinal microbiota composition	644:676	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	9	73	theme	microbial	1390:1398	arg1	diversity					1400:1408	microbial diversity	1390:1408	microbial diversity	1390:1408	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	3	74	theme	bacteria	345:352	arg1	One					314:316	One	314:316	One	314:316	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	3	74	theme	bacteria	345:352	arg1	bacteria					345:352	the most representative bacteria	321:352	the most representative bacteria involved in CRC	321:368	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	8	75	theme	AOM/DSS	1215:1221	arg1	group					1223:1227	ETBF colonized AOM/DSS group	1200:1227	ETBF colonized AOM/DSS group	1200:1227	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	8	76	theme	ETBF/AOM/DSS	1158:1169	arg1	group					1180:1184	ETBF/AOM/DSS + Z (60) group	1158:1184	ETBF/AOM/DSS + Z (60) group	1158:1184	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	5	77	theme	-treated	866:873	arg1	group					875:879	-treated group	866:879	-treated group	866:879	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	10	78	theme	taxonomic	1507:1515	arg1	abundance					1526:1534	the taxonomic relative abundance	1503:1534	the taxonomic relative abundance	1503:1534	However, after given zerumbone, the taxonomic relative abundance was increased.					
32958727	5	79	theme	ETBF	759:762	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	79	theme	ETBF	759:762	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	4	80	theme	sulphate	523:530	arg1	DSS					540:542	DSS	540:542	DSS	540:542	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	4	80	theme	sulphate	523:530	arg1	sodium					532:537	azoxymethane (AOM)/dextran sulphate sodium	496:537	azoxymethane (AOM)/dextran sulphate sodium (DSS)	496:543	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	0	81	theme	Microbiota	23:32	arg1	Composition					34:44	Gut Microbiota Composition	19:44	Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice	19:75	Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice.					
32958727	9	82	theme	ETBF	1441:1444	arg1	group					1464:1468	the ETBF colonized AOM/DSS group	1437:1468	the ETBF colonized AOM/DSS group	1437:1468	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	6	83	theme	region	954:959	arg1	V3-V4					961:965	16S rRNA gene region V3-V4	940:965	16S rRNA gene region V3-V4 for group comparison	940:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	3	84	theme	phylum	441:446	arg1	Bacteroidetes					448:460	phylum Bacteroidetes	441:460	phylum Bacteroidetes	441:460	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	7	85	theme	group	1038:1042	arg1	compositions					1000:1011	Microbiota compositions	989:1011	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone	989:1095	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	5	86	theme	mg	801:802	arg1	group					752:756	sham, ETBF colonized AOM/DSS group	723:756	group	752:756	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	86	theme	mg	801:802	arg1	kg-1					804:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1	759:807	ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60))	759:832	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	9	87	theme	AOM/DSS	1456:1462	arg1	group					1464:1468	the ETBF colonized AOM/DSS group	1437:1468	the ETBF colonized AOM/DSS group	1437:1468	In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group.					
32958727	6	88	theme	rRNA	944:947	arg1	V3-V4					961:965	16S rRNA gene region V3-V4	940:965	16S rRNA gene region V3-V4 for group comparison	940:986	We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison.					
32958727	0	89	theme	Colonized	54:62	arg1	Mice					72:75	ETBF Colonized AOM/DSS Mice	49:75	ETBF Colonized AOM/DSS Mice	49:75	Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice.					
32958727	3	90	theme	Bacteroides	389:399	arg1	ETBF					411:414	ETBF	411:414	ETBF	411:414	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	3	90	theme	Bacteroides	389:399	arg1	fragilis					401:408	enterotoxigenic Bacteroides fragilis	373:408	enterotoxigenic Bacteroides fragilis (ETBF)	373:415	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	3	90	theme	Bacteroides	389:399	arg1	species					420:426	a species	418:426	a species belonging to phylum Bacteroidetes	418:460	One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes.					
32958727	8	91	theme	normal	1269:1274	arg1	flora					1276:1280	a normal flora	1267:1280	a normal flora activated by zerumbone	1267:1303	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	8	91	theme	normal	1269:1274	arg1	fragilis					1249:1256	B. fragilis	1246:1256	B. fragilis	1246:1256	There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone.					
32958727	4	92	with	mice	486:489	arg1	compound					562:569	a compound	560:569	a compound with anti-bacterial effect	560:596	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	4	92	with	mice	486:489	arg1	DSS					540:542	DSS	540:542	DSS	540:542	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	4	92	with	mice	486:489	arg1	zerumbone					549:557	zerumbone	549:557	zerumbone	549:557	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	4	92	with	mice	486:489	arg1	sodium					532:537	azoxymethane (AOM)/dextran sulphate sodium	496:537	azoxymethane (AOM)/dextran sulphate sodium (DSS)	496:543	We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition.					
32958727	5	93	dep	kg-1	804:807	arg1	Z					826:826	Z (60)	826:831	Z (60)	826:831	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	5	93	dep	kg-1	804:807	arg1	DSS					820:822	ETBF/AOM/ DSS	810:822	ETBF/AOM/ DSS	810:822	Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg-1 (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg-1)-treated group.					
32958727	11	94	theme	microbial	1706:1714	arg1	composition					1716:1726	gut microbial composition	1702:1726	gut microbial composition	1702:1726	These findings suggest that zerumbone not only influenced the microbial diversity and richness, but also could be helpful for enhancing the balance of gut microbial composition.					
32958727	7	95	theme	AOM/DSS	1063:1069	arg1	group					1071:1075	ETBF colonized AOM/DSS group	1048:1075	ETBF colonized AOM/DSS group	1048:1075	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
32958727	7	96	theme	group	1071:1075	arg1	compositions					1000:1011	Microbiota compositions	989:1011	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone	989:1095	Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different.					
34367349	6	0	theme	glycogene	1080:1088	arg1	classification					1098:1111	glycogene pathway classification	1080:1111	glycogene pathway classification	1080:1111	Upon applying the Fisher's exact test along with glycogene pathway classification, we identified TFs that may specifically regulate the biosynthesis of individual glycan types.					
34367349	3	1	theme	chromatin	509:517	arg1	ChIP-Seq					551:558	chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data	509:576	ChIP-Seq	551:558	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	9	2	theme	TF-glycogene	1559:1570	arg1	relationships					1572:1584	TF-glycogene relationships	1559:1584	TF-glycogene relationships	1559:1584	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	0	3	theme	signaling	89:97	arg1	pathways					99:106	signaling pathways	89:106	signaling pathways regulating glycan biosynthesis	89:137	A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis.					
34367349	3	4	theme	immunoprecipitation	519:537	arg1	ChIP-Seq					551:558	chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data	509:576	ChIP-Seq	551:558	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	0	5	theme	glycan	119:124	arg1	biosynthesis					126:137	glycan biosynthesis	119:137	glycan biosynthesis	119:137	A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis.					
34367349	8	6	theme	cancer	1464:1469	arg1	progression					1479:1489	human luminal and basal breast cancer disease progression	1433:1489	human luminal and basal breast cancer disease progression	1433:1489	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	8	7	theme	breast	1457:1462	arg1	progression					1479:1489	human luminal and basal breast cancer disease progression	1433:1489	human luminal and basal breast cancer disease progression	1433:1489	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	4	8	theme	Atlas	801:805	arg1	types					821:825	the Cancer Genome Atlas (TCGA) cancer types	783:825	29 the Cancer Genome Atlas (TCGA) cancer types	780:825	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	6	9	theme	individual	1183:1192	arg1	types					1201:1205	individual glycan types	1183:1205	individual glycan types	1183:1205	Upon applying the Fisher's exact test along with glycogene pathway classification, we identified TFs that may specifically regulate the biosynthesis of individual glycan types.					
34367349	7	10	with	Integration	1208:1218	arg1	knowledge					1237:1245	Reactome DB knowledge	1225:1245	Reactome DB knowledge	1225:1245	Integration with Reactome DB knowledge provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state.					
34367349	4	11	theme	Genome	794:799	arg1	types					821:825	the Cancer Genome Atlas (TCGA) cancer types	783:825	29 the Cancer Genome Atlas (TCGA) cancer types	780:825	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	3	12	theme	RNA-Seq	565:571	arg1	data					573:576	chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data	509:576	data	573:576	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	9	13	dep	approach	1538:1545	arg1	describe					1550:1557	describe	1550:1557	to describe TF-glycogene relationships	1547:1584	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	2	14	theme	glycosylation	340:352	arg1	pathways					354:361	related glycosylation pathways	332:361	related glycosylation pathways	332:361	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown.					
34367349	5	15	theme	TF	941:942	arg1	expression					944:953	single TF expression	934:953	single TF expression	934:953	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	5	16	from	changes	923:929	arg1	expression					944:953	single TF expression	934:953	single TF expression	934:953	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	4	17	theme	TCGA	808:811	arg1	types					821:825	the Cancer Genome Atlas (TCGA) cancer types	783:825	29 the Cancer Genome Atlas (TCGA) cancer types	780:825	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	1	18	theme	glycan	202:207	arg1	biosynthesis					209:220	glycan biosynthesis	202:220	glycan biosynthesis	202:220	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	5	19	theme	single	934:939	arg1	expression					944:953	single TF expression	934:953	single TF expression	934:953	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	8	20	theme	specific	1405:1412	arg1	focus					1414:1418	specific focus	1405:1418	specific focus	1405:1418	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	3	21	theme	Cistrome	461:468	arg1	database					477:484	the Cistrome Cancer database	457:484	the Cistrome Cancer database (DB)	457:489	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	3	21	theme	Cistrome	461:468	arg1	DB					487:488	DB	487:488	DB	487:488	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	0	22	theme	systems-based	2:14	arg1	framework					16:24	A systems-based framework	0:24	A systems-based framework	0:24	A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis.					
34367349	9	23	theme	starting	1591:1598	arg1	approach					1538:1545	a computational approach	1522:1545	a computational approach to describe TF-glycogene relationships	1522:1584	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	9	23	theme	starting	1591:1598	arg1	point					1600:1604	the starting point	1587:1604	the starting point for experimental system-wide validation	1587:1644	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	2	24	theme	related	332:338	arg1	pathways					354:361	related glycosylation pathways	332:361	related glycosylation pathways	332:361	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown.					
34367349	7	25	theme	DB	1234:1235	arg1	knowledge					1237:1245	Reactome DB knowledge	1225:1245	Reactome DB knowledge	1225:1245	Integration with Reactome DB knowledge provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state.					
34367349	5	26	theme	so-called	884:892	arg1	communities					894:904	so-called communities	884:904	so-called communities	884:904	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	3	27	theme	regulatory	592:601	arg1	relationships					603:615	regulatory relationships	592:615	regulatory relationships	592:615	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	5	28	theme	TF-glycogene	834:845	arg1	interactions					847:858	TF-glycogene interactions	834:858	TF-glycogene interactions	834:858	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	4	29	theme	Cancer	787:792	arg1	types					821:825	the Cancer Genome Atlas (TCGA) cancer types	783:825	29 the Cancer Genome Atlas (TCGA) cancer types	780:825	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	4	30	theme	significant	659:669	arg1	relationships					684:696	22,654 potentially significant TF-glycogene relationships	640:696	22,654 potentially significant TF-glycogene relationships	640:696	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	6	31	theme	types	1201:1205	arg1	biosynthesis					1167:1178	the biosynthesis	1163:1178	the biosynthesis of individual glycan types	1163:1205	Upon applying the Fisher's exact test along with glycogene pathway classification, we identified TFs that may specifically regulate the biosynthesis of individual glycan types.					
34367349	5	32	theme	carbohydrate	1006:1017	arg1	structures					1019:1028	multiple carbohydrate structures	997:1028	multiple carbohydrate structures	997:1028	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	4	33	theme	unique	740:745	arg1	TFs					747:749	526 unique TFs	736:749	526 unique TFs	736:749	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	1	34	theme	posttranslational	166:182	arg1	modification					184:195	a common posttranslational modification	157:195	a common posttranslational modification	157:195	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	1	34	theme	posttranslational	166:182	arg1	Glycosylation					140:152	Glycosylation	140:152	Glycosylation	140:152	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	9	35	theme	computational	1524:1536	arg1	point					1600:1604	the starting point	1587:1604	the starting point for experimental system-wide validation	1587:1644	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	9	35	theme	computational	1524:1536	arg1	approach					1538:1545	a computational approach	1522:1545	a computational approach to describe TF-glycogene relationships	1522:1584	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	3	36	theme	Cancer	470:475	arg1	database					477:484	the Cistrome Cancer database	457:484	the Cistrome Cancer database (DB)	457:489	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	3	36	theme	Cancer	470:475	arg1	DB					487:488	DB	487:488	DB	487:488	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	3	37	theme	TF-glycogene	425:436	arg1	relationships					438:450	TF-glycogene relationships	425:450	TF-glycogene relationships	425:450	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	8	38	theme	luminal	1439:1445	arg1	progression					1479:1489	human luminal and basal breast cancer disease progression	1433:1489	human luminal and basal breast cancer disease progression	1433:1489	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	4	39	theme	TF-glycogene	671:682	arg1	relationships					684:696	22,654 potentially significant TF-glycogene relationships	640:696	22,654 potentially significant TF-glycogene relationships	640:696	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	0	40	theme	transcription	63:75	arg1	factors					77:83	putative transcription factors	54:83	putative transcription factors	54:83	A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis.					
34367349	5	41	theme	multiple	997:1004	arg1	structures					1019:1028	multiple carbohydrate structures	997:1028	multiple carbohydrate structures	997:1028	Here, TF-glycogene interactions appeared in clusters or so-called communities, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures.					
34367349	4	42	theme	341	755:757	arg1	glycogenes					759:768	341 glycogenes	755:768	341 glycogenes	755:768	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	0	43	theme	putative	54:61	arg1	factors					77:83	putative transcription factors	54:83	putative transcription factors	54:83	A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis.					
34367349	8	44	theme	basal	1451:1455	arg1	progression					1479:1489	human luminal and basal breast cancer disease progression	1433:1489	human luminal and basal breast cancer disease progression	1433:1489	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	8	45	theme	analysis	1349:1356	arg1	results					1358:1364	analysis results	1349:1364	analysis results	1349:1364	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	7	46	theme	cellular	1311:1318	arg1	state					1334:1338	cellular glycosylation state	1311:1338	cellular glycosylation state	1311:1338	Integration with Reactome DB knowledge provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state.					
34367349	8	47	theme	disease	1471:1477	arg1	progression					1479:1489	human luminal and basal breast cancer disease progression	1433:1489	human luminal and basal breast cancer disease progression	1433:1489	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	4	48	theme	cancer	814:819	arg1	types					821:825	the Cancer Genome Atlas (TCGA) cancer types	783:825	29 the Cancer Genome Atlas (TCGA) cancer types	780:825	In total, we observed 22,654 potentially significant TF-glycogene relationships, which include interactions involving 526 unique TFs and 341 glycogenes that span 29 the Cancer Genome Atlas (TCGA) cancer types.					
34367349	7	49	theme	glycosylation	1320:1332	arg1	state					1334:1338	cellular glycosylation state	1311:1338	cellular glycosylation state	1311:1338	Integration with Reactome DB knowledge provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state.					
34367349	6	50	theme	glycan	1194:1199	arg1	types					1201:1205	individual glycan types	1183:1205	individual glycan types	1183:1205	Upon applying the Fisher's exact test along with glycogene pathway classification, we identified TFs that may specifically regulate the biosynthesis of individual glycan types.					
34367349	1	51	theme	glycogenes	247:256	arg1	glycogenes					247:256	glycogenes	247:256	glycogenes	247:256	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	1	51	theme	glycogenes	247:256	arg1	set					240:242	a set	238:242	a set of glycogenes	238:256	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	2	52	theme	factors	285:291	arg1	role					263:266	The role	259:266	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways	259:361	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown.					
34367349	2	52	theme	factors	285:291	arg1	unknown					374:380	unknown	374:380	unknown	374:380	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown.					
34367349	1	53	theme	common	159:164	arg1	modification					184:195	a common posttranslational modification	157:195	a common posttranslational modification	157:195	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	1	53	theme	common	159:164	arg1	Glycosylation					140:152	Glycosylation	140:152	Glycosylation	140:152	Glycosylation is a common posttranslational modification, and glycan biosynthesis is regulated by a set of glycogenes.					
34367349	8	54	theme	cancer	1391:1396	arg1	types					1398:1402	all 29 cancer types	1384:1402	all 29 cancer types	1384:1402	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	6	55	theme	exact	1058:1062	arg1	test					1064:1067	the Fisher's exact test	1045:1067	the Fisher's exact test	1045:1067	Upon applying the Fisher's exact test along with glycogene pathway classification, we identified TFs that may specifically regulate the biosynthesis of individual glycan types.					
34367349	7	56	theme	cell-signaling	1276:1289	arg1	pathways					1291:1298	cell-signaling pathways	1276:1298	cell-signaling pathways	1276:1298	Integration with Reactome DB knowledge provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state.					
34367349	8	57	theme	human	1433:1437	arg1	progression					1479:1489	human luminal and basal breast cancer disease progression	1433:1489	human luminal and basal breast cancer disease progression	1433:1489	Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression.					
34367349	2	58	theme	transcription	271:283	arg1	TFs					294:296	TFs	294:296	TFs	294:296	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown.					
34367349	2	58	theme	transcription	271:283	arg1	factors					285:291	transcription factors	271:291	transcription factors (TFs)	271:297	The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown.					
34367349	6	59	theme	pathway	1090:1096	arg1	classification					1098:1111	glycogene pathway classification	1080:1111	glycogene pathway classification	1080:1111	Upon applying the Fisher's exact test along with glycogene pathway classification, we identified TFs that may specifically regulate the biosynthesis of individual glycan types.					
34367349	3	60	theme	sequencing	539:548	arg1	ChIP-Seq					551:558	chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data	509:576	ChIP-Seq	551:558	In this work, we performed data mining of TF-glycogene relationships from the Cistrome Cancer database (DB), which integrates chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA-Seq data to constitute regulatory relationships.					
34367349	9	61	theme	experimental	1610:1621	arg1	validation					1635:1644	experimental system-wide validation	1610:1644	experimental system-wide validation	1610:1644	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	9	62	theme	system-wide	1623:1633	arg1	validation					1635:1644	experimental system-wide validation	1610:1644	experimental system-wide validation	1610:1644	Overall, the article presents a computational approach to describe TF-glycogene relationships, the starting point for experimental system-wide validation.					
34367349	7	63	theme	Reactome	1225:1232	arg1	knowledge					1237:1245	Reactome DB knowledge	1225:1245	Reactome DB knowledge	1225:1245	Integration with Reactome DB knowledge provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state.					
34192263	6	0	from	trimer	916:921	arg1	conformation					935:946	a single conformation	926:946	a single conformation	926:946	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	6	1	theme	symmetric	906:914	arg1	trimer					916:921	a three-fold symmetric trimer	893:921	a three-fold symmetric trimer in a single conformation	893:946	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	9	2	theme	future	1314:1319	arg1	studies					1321:1327	future studies	1314:1327	future studies of spike proteins with receptors, antibodies or drugs	1314:1381	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	2	3	theme	mild	349:352	arg1	symptoms					372:379	mild upper respiratory symptoms	349:379	mild upper respiratory symptoms in children, elderly and immunocompromised individuals	349:434	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	5	4	theme	virions	719:725	arg1	imaging					698:704	single-particle cryo-EM imaging	674:704	single-particle cryo-EM imaging of vitrified virions without chemical fixative	674:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	0	5	theme	trimer	74:79	arg1	structure					33:41	A 3.4-Å cryo-electron microscopy structure	0:41	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer	0:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	1	6	dep	10	270:271	arg1	to					267:268	to	267:268	to	267:268	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	5	7	theme	spike	634:638	arg1	trimer					644:649	HCoV-NL63 spike (S) trimer	624:649	HCoV-NL63 spike (S) trimer	624:649	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	7	8	from	sites	1049:1053	arg1	density					1032:1038	density	1032:1038	density at other sites	1032:1053	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	7	8	from	sites	1049:1053	arg1	structures					1084:1093	different glycan structures	1067:1093	different glycan structures	1067:1093	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	8	9	from	that	1166:1169	arg1	arrangement					1107:1117	The domain arrangement	1096:1117	The domain arrangement within a protomer	1096:1135	The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor.					
34192263	8	9	from	that	1166:1169	arg1	different					1151:1159	different	1151:1159	different	1151:1159	The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor.					
34192263	9	10	theme	coronavirus	1411:1421	arg1	particles					1423:1431	the coronavirus particles	1407:1431	the coronavirus particles	1407:1431	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	3	11	dep	severe	469:474	arg1	respiratory					482:492	respiratory	482:492	respiratory	482:492	It has also been shown to cause severe lower respiratory illness.					
34192263	5	12	from	resolution	660:669	arg1	structure					611:619	the in situ structure	599:619	the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative	599:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	5	13	theme	S	641:641	arg1	trimer					644:649	HCoV-NL63 spike (S) trimer	624:649	HCoV-NL63 spike (S) trimer	624:649	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	0	14	theme	NL63	120:123	arg1	particles					131:139	vitrified NL63 virus particles	110:139	vitrified NL63 virus particles	110:139	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	5	15	theme	in	603:604	arg1	structure					611:619	the in situ structure	599:619	the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative	599:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	9	16	theme	particles	1423:1431	arg1	state					1398:1402	the native state	1387:1402	the native state of the coronavirus particles	1387:1431	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	0	17	theme	vitrified	110:118	arg1	particles					131:139	vitrified NL63 virus particles	110:139	vitrified NL63 virus particles	110:139	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	7	18	theme	glycan	1077:1082	arg1	structures					1084:1093	different glycan structures	1067:1093	different glycan structures	1067:1093	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	5	19	theme	vitrified	709:717	arg1	virions					719:725	vitrified virions	709:725	vitrified virions without chemical fixative	709:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	0	20	theme	3.4-Å	2:6	arg1	microscopy					22:31	A 3.4-Å cryo-electron microscopy	0:31	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer	0:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	6	21	theme	trimer	870:875	arg1	trimer					870:875	HCoV-NL63 S trimer	858:875	HCoV-NL63 S trimer	858:875	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	6	21	theme	trimer	870:875	arg1	ectodomain					844:853	the biochemically purified ectodomain	817:853	the biochemically purified ectodomain	817:853	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	9	22	with	proteins	1338:1345	arg1	receptors					1352:1360	receptors	1352:1360	receptors	1352:1360	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	9	22	with	proteins	1338:1345	arg1	drugs					1377:1381	drugs	1377:1381	drugs	1377:1381	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	9	22	with	proteins	1338:1345	arg1	antibodies					1363:1372	antibodies	1363:1372	antibodies	1363:1372	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	5	23	dep	in	603:604	arg1	situ					606:609	situ	606:609	situ	606:609	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	7	24	theme	other	1043:1047	arg1	sites					1049:1053	other sites	1043:1053	other sites	1043:1053	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	7	25	theme	different	1067:1075	arg1	structures					1084:1093	different glycan structures	1067:1093	different glycan structures	1067:1093	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	1	26	theme	enveloped	183:191	arg1	coronavirus					148:158	Human coronavirus NL63	142:163	Human coronavirus NL63 (HCoV-NL63)	142:175	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	1	26	theme	enveloped	183:191	arg1	pathogen					193:200	an enveloped pathogen	180:200	an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases	180:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	0	27	theme	microscopy	22:31	arg1	structure					33:41	A 3.4-Å cryo-electron microscopy structure	0:41	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer	0:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	4	28	theme	viral	538:542	arg1	entry					544:548	viral entry	538:548	viral entry	538:548	NL63 shares ACE2 as a receptor for viral entry with SARS-CoV-1 and SARS-CoV-2.					
34192263	0	29	theme	cryo-electron	8:20	arg1	microscopy					22:31	A 3.4-Å cryo-electron microscopy	0:31	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer	0:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	9	30	theme	spike	1332:1336	arg1	proteins					1338:1345	spike proteins	1332:1345	spike proteins with receptors, antibodies or drugs	1332:1381	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	0	31	theme	virus	125:129	arg1	particles					131:139	vitrified NL63 virus particles	110:139	vitrified NL63 virus particles	110:139	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	6	32	theme	S	868:868	arg1	trimer					870:875	HCoV-NL63 S trimer	858:875	HCoV-NL63 S trimer	858:875	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	2	33	theme	immunocompromised	406:422	arg1	individuals					424:434	immunocompromised individuals	406:434	immunocompromised individuals	406:434	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	9	34	theme	proteins	1338:1345	arg1	studies					1321:1327	future studies	1314:1327	future studies of spike proteins with receptors, antibodies or drugs	1314:1381	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	5	35	theme	single-particle	674:688	arg1	imaging					698:704	single-particle cryo-EM imaging	674:704	single-particle cryo-EM imaging of vitrified virions without chemical fixative	674:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	1	36	theme	annual	281:286	arg1	diseases					300:307	all annual respiratory diseases	277:307	all annual respiratory diseases	277:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	5	37	theme	cryo-EM	690:696	arg1	imaging					698:704	single-particle cryo-EM imaging	674:704	single-particle cryo-EM imaging of vitrified virions without chemical fixative	674:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	1	38	theme	family	209:214	arg1	Coronaviridae					216:228	the family Coronaviridae	205:228	the family Coronaviridae	205:228	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	0	39	theme	human	50:54	arg1	trimer					74:79	the human coronavirus spike trimer	46:79	the human coronavirus spike trimer	46:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	1	40	theme	respiratory	288:298	arg1	diseases					300:307	all annual respiratory diseases	277:307	all annual respiratory diseases	277:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	5	41	theme	chemical	735:742	arg1	fixative					744:751	chemical fixative	735:751	chemical fixative	735:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	2	42	from	symptoms	372:379	arg1	children					384:391	children	384:391	children	384:391	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	2	42	from	symptoms	372:379	arg1	elderly					394:400	elderly	394:400	elderly	394:400	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	2	42	from	symptoms	372:379	arg1	individuals					424:434	immunocompromised individuals	406:434	immunocompromised individuals	406:434	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	1	43	theme	diseases	300:307	arg1	diseases					300:307	all annual respiratory diseases	277:307	all annual respiratory diseases	277:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	1	43	theme	diseases	300:307	arg1	%					272:272	up to 10%	264:272	up to 10% of all annual respiratory diseases	264:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	5	44	theme	trimer	644:649	arg1	structure					611:619	the in situ structure	599:619	the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative	599:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	6	45	theme	purified	835:842	arg1	trimer					870:875	HCoV-NL63 S trimer	858:875	HCoV-NL63 S trimer	858:875	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	6	45	theme	purified	835:842	arg1	ectodomain					844:853	the biochemically purified ectodomain	817:853	the biochemically purified ectodomain	817:853	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
34192263	7	46	theme	glycosylation	997:1009	arg1	sites					1011:1015	observed glycosylation sites	988:1015	observed glycosylation sites	988:1015	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	7	47	gly	glycosylation	997:1009	arg2	sites					1011:1015	observed glycosylation sites	988:1015	observed glycosylation sites	988:1015	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	8	48	theme	domain	1100:1105	arg1	arrangement					1107:1117	The domain arrangement	1096:1117	The domain arrangement within a protomer	1096:1135	The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor.					
34192263	8	48	theme	domain	1100:1105	arg1	different					1151:1159	different	1151:1159	different	1151:1159	The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor.					
34192263	3	49	theme	severe	469:474	arg1	illness					494:500	severe lower respiratory illness	469:500	severe lower respiratory illness	469:500	It has also been shown to cause severe lower respiratory illness.					
34192263	1	50	theme	Coronaviridae	216:228	arg1	coronavirus					148:158	Human coronavirus NL63	142:163	Human coronavirus NL63 (HCoV-NL63)	142:175	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	1	50	theme	Coronaviridae	216:228	arg1	pathogen					193:200	an enveloped pathogen	180:200	an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases	180:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	5	51	theme	3.4-Å	654:658	arg1	resolution					660:669	3.4-Å resolution	654:669	3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative	654:751	Here, we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-Å resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative.					
34192263	9	52	theme	native	1391:1396	arg1	state					1398:1402	the native state	1387:1402	the native state of the coronavirus particles	1387:1431	This structure provides the basis for future studies of spike proteins with receptors, antibodies or drugs, in the native state of the coronavirus particles.					
34192263	1	53	theme	Human	142:146	arg1	pathogen					193:200	an enveloped pathogen	180:200	an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases	180:307	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	1	53	theme	Human	142:146	arg1	coronavirus					148:158	Human coronavirus NL63	142:163	Human coronavirus NL63 (HCoV-NL63)	142:175	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	1	53	theme	Human	142:146	arg1	HCoV-NL63					166:174	HCoV-NL63	166:174	HCoV-NL63	166:174	Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases.					
34192263	0	54	theme	spike	68:72	arg1	trimer					74:79	the human coronavirus spike trimer	46:79	the human coronavirus spike trimer	46:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	7	55	theme	observed	988:995	arg1	sites					1011:1015	observed glycosylation sites	988:1015	observed glycosylation sites	988:1015	In addition to previously proposed and observed glycosylation sites, our map shows density at other sites, as well as different glycan structures.					
34192263	2	56	theme	respiratory	360:370	arg1	symptoms					372:379	mild upper respiratory symptoms	349:379	mild upper respiratory symptoms in children, elderly and immunocompromised individuals	349:434	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	8	57	theme	different	1213:1221	arg1	requirements					1223:1234	their different requirements	1207:1234	their different requirements for activating binding to the receptor	1207:1273	The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor.					
34192263	0	58	theme	coronavirus	56:66	arg1	trimer					74:79	the human coronavirus spike trimer	46:79	the human coronavirus spike trimer	46:79	A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles.					
34192263	2	59	theme	upper	354:358	arg1	symptoms					372:379	mild upper respiratory symptoms	349:379	mild upper respiratory symptoms in children, elderly and immunocompromised individuals	349:434	HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals.					
34192263	6	60	theme	single	928:933	arg1	conformation					935:946	a single conformation	926:946	a single conformation	926:946	It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a three-fold symmetric trimer in a single conformation.					
31954122	7	0	theme	physicochemical	1659:1673	arg1	properties					1690:1699	the excellent physicochemical and structural properties	1645:1699	the excellent physicochemical and structural properties of commercial chitin	1645:1720	The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin.					
31954122	3	1	theme	acid	687:690	arg1	quantities					692:701	the organic acid quantities	675:701	the organic acid quantities	675:701	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	2	theme	inoculum	534:541	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	2	theme	inoculum	534:541	arg1	pH 6.5					561:566	initial pH 6.5	553:566	initial pH 6.5	553:566	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	2	theme	inoculum	534:541	arg1	48 h					637:640	cultured time 48 h	623:640	cultured time 48 h	623:640	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	2	theme	inoculum	534:541	arg1	%					620:620	glucose concentration 5%	597:620	glucose concentration 5%	597:620	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	2	theme	inoculum	534:541	arg1	37 °C					590:594	cultured temperature 37 °C	569:594	cultured temperature 37 °C	569:594	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	3	theme	broth	729:733	arg1	conditions					522:531	the cultured conditions	509:531	the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides	509:669	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	3	theme	broth	729:733	arg1	types					707:711	types	707:711	types	707:711	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	3	theme	broth	729:733	arg1	quantities					692:701	the organic acid quantities	675:701	the organic acid quantities	675:701	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	6	4	theme	infrared	1414:1421	arg1	FT-IR					1437:1441	FT-IR	1437:1441	FT-IR	1437:1441	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	6	4	theme	infrared	1414:1421	arg1	spectrometer					1423:1434	infrared spectrometer	1414:1434	infrared spectrometer (FT-IR)	1414:1442	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	2	5	dep	microorganisms	258:271	arg1	amyloliquefaciens					311:327	Bacillus amyloliquefaciens	302:327	Bacillus amyloliquefaciens (BA01)	302:334	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	2	5	dep	microorganisms	258:271	arg1	BA01					330:333	BA01	330:333	BA01	330:333	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	2	5	dep	microorganisms	258:271	arg1	rhamnoides					287:296	Lactobacillus rhamnoides	273:296	Lactobacillus rhamnoides	273:296	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	9	6	theme	animal	1975:1980	arg1	feed					1982:1985	animal feed	1975:1985	animal feed	1975:1985	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	1	7	theme	shells	191:196	arg1	powders					198:204	shrimp shells powders	184:204	shrimp shells powders (SSP)	184:210	Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation.					
31954122	1	7	theme	shells	191:196	arg1	SSP					207:209	SSP	207:209	SSP	207:209	Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation.					
31954122	9	8	used	used	1946:1949	arg2	broth					1900:1904	the fermentation broth	1883:1904	the fermentation broth	1883:1904	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	9	8	used	used	1946:1949	arg2	nutrient					1963:1970	a health nutrient	1954:1970	a health nutrient in animal feed	1954:1985	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	3	9	theme	Lactobacillus	738:750	arg1	rhamnoides					752:761	Lactobacillus rhamnoides	738:761	Lactobacillus rhamnoides	738:761	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	10	theme	cultured	569:576	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	10	theme	cultured	569:576	arg1	37 °C					590:594	cultured temperature 37 °C	569:594	cultured temperature 37 °C	569:594	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	6	11	theme	commercial	1363:1372	arg1	chitin					1374:1379	the commercial chitin	1359:1379	the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM)	1359:1577	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	8	12	theme	shrimp	1762:1767	arg1	use					1755:1757	full use	1750:1757	full use of shrimp and crab shells resources	1750:1793	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	1	13	theme	successive	215:224	arg1	fermentation					235:246	successive two-step fermentation	215:246	successive two-step fermentation	215:246	Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation.					
31954122	8	14	theme	crab	1773:1776	arg1	shells					1778:1783	crab shells	1773:1783	crab shells	1773:1783	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	5	15	theme	BA01	983:986	arg1	%					1009:1009	inoculum level 6%	993:1009	inoculum level 6%	993:1009	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	15	theme	BA01	983:986	arg1	conditions					969:978	the optimal cultured conditions	948:978	the optimal cultured conditions of BA01	948:986	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	3	16	theme	glucose	597:603	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	16	theme	glucose	597:603	arg1	%					620:620	glucose concentration 5%	597:620	glucose concentration 5%	597:620	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	6	17	theme	X-ray	1445:1449	arg1	XRD					1464:1466	XRD	1464:1466	XRD	1464:1466	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	6	17	theme	X-ray	1445:1449	arg1	diffraction					1451:1461	X-ray diffraction	1445:1461	X-ray diffraction (XRD)	1445:1467	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	6	18	theme	13C	1516:1518	arg1	spectra					1531:1537	Solid-state 13C CP/MAS-NMR spectra	1504:1537	Solid-state 13C CP/MAS-NMR spectra	1504:1537	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	5	19	theme	DP	1192:1193	arg1	efficiency					1195:1204	the DP efficiency	1188:1204	the DP efficiency	1188:1204	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	19	theme	DP	1192:1193	arg1	%					1214:1214	96.8%	1210:1214	96.8%	1210:1214	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	20	theme	inoculum	993:1000	arg1	%					1009:1009	inoculum level 6%	993:1009	inoculum level 6%	993:1009	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	20	theme	inoculum	993:1000	arg1	conditions					969:978	the optimal cultured conditions	948:978	the optimal cultured conditions of BA01	948:986	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	3	21	theme	time	632:635	arg1	48 h					637:640	cultured time 48 h	623:640	cultured time 48 h	623:640	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	21	theme	time	632:635	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	5	22	from	protein	1221:1227	arg1	residue					1242:1248	the final residue	1232:1248	the final residue	1232:1248	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	4	23	from	ash	860:862	arg1	residue					877:883	the final residue	867:883	the final residue	867:883	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	2	24	theme	best	253:256	arg1	microorganisms					258:271	The best microorganisms	249:271	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP)	249:386	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	0	25	theme	successive	63:72	arg1	fermentation					83:94	successive two-step fermentation	63:94	successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens	63:155	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	5	26	theme	glucose	1056:1062	arg1	%					1079:1079	glucose concentration 4%	1056:1079	glucose concentration 4%	1056:1079	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	4	27	theme	organic	908:914	arg1	acid					916:919	the main organic acid	899:919	the main organic acid	899:919	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	4	27	theme	organic	908:914	arg1	acid					932:935	lactic acid	925:935	lactic acid	925:935	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	5	28	theme	cultured	1028:1035	arg1	37 °C					1049:1053	cultured temperature 37 °C	1028:1053	cultured temperature 37 °C	1028:1053	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	6	29	theme	Thermogravimetric	1470:1486	arg1	TGA					1498:1500	TGA	1498:1500	TGA	1498:1500	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	6	29	theme	Thermogravimetric	1470:1486	arg1	analysis					1488:1495	Thermogravimetric analysis	1470:1495	Thermogravimetric analysis (TGA)	1470:1501	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	3	30	dep	conditions	522:531	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	30	dep	conditions	522:531	arg1	pH 6.5					561:566	initial pH 6.5	553:566	initial pH 6.5	553:566	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	30	dep	conditions	522:531	arg1	48 h					637:640	cultured time 48 h	623:640	cultured time 48 h	623:640	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	30	dep	conditions	522:531	arg1	%					620:620	glucose concentration 5%	597:620	glucose concentration 5%	597:620	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	30	dep	conditions	522:531	arg1	37 °C					590:594	cultured temperature 37 °C	569:594	cultured temperature 37 °C	569:594	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	6	31	theme	electron	1553:1560	arg1	SEM					1574:1576	SEM	1574:1576	SEM	1574:1576	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	6	31	theme	electron	1553:1560	arg1	microscope					1562:1571	Scanning electron microscope	1544:1571	Scanning electron microscope (SEM)	1544:1577	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	5	32	theme	final	1236:1240	arg1	residue					1242:1248	the final residue	1232:1248	the final residue	1232:1248	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	0	33	theme	Chitin	0:5	arg1	extraction					7:16	Chitin extraction	0:16	Chitin extraction from shrimp (Litopenaeus vannamei)	0:51	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	5	34	theme	cultured	1086:1093	arg1	84 h.					1100:1104	cultured time 84 h.	1086:1104	cultured time 84 h.	1086:1104	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	9	35	theme	fermentation	1887:1898	arg1	broth					1900:1904	the fermentation broth	1883:1904	the fermentation broth	1883:1904	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	9	35	theme	fermentation	1887:1898	arg1	nutrient					1963:1970	a health nutrient	1954:1970	a health nutrient in animal feed	1954:1985	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	3	36	theme	Lactobacillus	646:658	arg1	rhamnoides					660:669	Lactobacillus rhamnoides	646:669	Lactobacillus rhamnoides	646:669	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	2	37	theme	fermentation	418:429	arg1	conditions					431:440	the optimal fermentation conditions	406:440	the optimal fermentation conditions for two-step fermentation	406:466	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	5	38	theme	cultured	960:967	arg1	%					1009:1009	inoculum level 6%	993:1009	inoculum level 6%	993:1009	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	38	theme	cultured	960:967	arg1	conditions					969:978	the optimal cultured conditions	948:978	the optimal cultured conditions of BA01	948:986	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	7	39	theme	commercial	1704:1713	arg1	chitin					1715:1720	commercial chitin	1704:1720	commercial chitin	1704:1720	The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin.					
31954122	9	40	theme	nutritional	1915:1925	arg1	value					1927:1931	high nutritional value	1910:1931	high nutritional value	1910:1931	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	3	41	theme	cultured	513:520	arg1	conditions					522:531	the cultured conditions	509:531	the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides	509:669	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	42	theme	organic	679:685	arg1	quantities					692:701	the organic acid quantities	675:701	the organic acid quantities	675:701	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	5	43	theme	chitin	1268:1273	arg1	yield					1275:1279	the chitin yield	1264:1279	the chitin yield	1264:1279	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	43	theme	chitin	1268:1273	arg1	%					1289:1289	19.6%	1285:1289	19.6%	1285:1289	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	1	44	theme	two-step	226:233	arg1	fermentation					235:246	successive two-step fermentation	215:246	successive two-step fermentation	215:246	Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation.					
31954122	9	45	contain	had	1906:1908	arg1	broth					1900:1904	the fermentation broth	1883:1904	the fermentation broth	1883:1904	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	9	45	contain	had	1906:1908	arg2	value					1927:1931	high nutritional value	1910:1931	high nutritional value	1910:1931	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	9	45	contain	had	1906:1908	arg1	nutrient					1963:1970	a health nutrient	1954:1970	a health nutrient in animal feed	1954:1985	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	8	46	theme	acid	1806:1809	arg1	composition					1811:1821	the amino acid composition	1796:1821	the amino acid composition of fermentation broth	1796:1843	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	3	47	theme	level	543:547	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	47	theme	level	543:547	arg1	pH 6.5					561:566	initial pH 6.5	553:566	initial pH 6.5	553:566	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	47	theme	level	543:547	arg1	48 h					637:640	cultured time 48 h	623:640	cultured time 48 h	623:640	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	47	theme	level	543:547	arg1	%					620:620	glucose concentration 5%	597:620	glucose concentration 5%	597:620	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	47	theme	level	543:547	arg1	37 °C					590:594	cultured temperature 37 °C	569:594	cultured temperature 37 °C	569:594	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	7	48	theme	excellent	1649:1657	arg1	properties					1690:1699	the excellent physicochemical and structural properties	1645:1699	the excellent physicochemical and structural properties of commercial chitin	1645:1720	The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin.					
31954122	7	49	dep	chitin	1603:1608	arg1	maintains					1635:1643	maintains	1635:1643	maintains the excellent physicochemical and structural properties of commercial chitin	1635:1720	The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin.					
31954122	9	50	theme	health	1956:1961	arg1	food					1993:1996	food	1993:1996	food	1993:1996	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	9	50	theme	health	1956:1961	arg1	broth					1900:1904	the fermentation broth	1883:1904	the fermentation broth	1883:1904	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	9	50	theme	health	1956:1961	arg1	nutrient					1963:1970	a health nutrient	1954:1970	a health nutrient in animal feed	1954:1985	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	3	51	theme	initial	553:559	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	51	theme	initial	553:559	arg1	pH 6.5					561:566	initial pH 6.5	553:566	initial pH 6.5	553:566	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	1	52	theme	shrimp	184:189	arg1	powders					198:204	shrimp shells powders	184:204	shrimp shells powders (SSP)	184:210	Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation.					
31954122	1	52	theme	shrimp	184:189	arg1	SSP					207:209	SSP	207:209	SSP	207:209	Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation.					
31954122	4	53	theme	DM	828:829	arg1	efficiency					831:840	the DM efficiency	824:840	the DM efficiency	824:840	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	4	53	theme	DM	828:829	arg1	%					850:850	97.5%	846:850	97.5%	846:850	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	6	54	dep	Fourier	1396:1402	arg1	transform					1404:1412	transform	1404:1412	transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM)	1404:1577	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	3	55	theme	fermentation	716:727	arg1	broth					729:733	fermentation broth	716:733	fermentation broth of Lactobacillus rhamnoides	716:761	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	8	56	theme	full	1750:1753	arg1	use					1755:1757	full use	1750:1757	full use of shrimp and crab shells resources	1750:1793	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	3	57	theme	temperature	578:588	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	57	theme	temperature	578:588	arg1	37 °C					590:594	cultured temperature 37 °C	569:594	cultured temperature 37 °C	569:594	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	6	58	theme	scanning	1387:1394	arg1	Fourier					1396:1402	scanning Fourier	1387:1402	scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM)	1387:1577	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	0	59	with	fermentation	83:94	arg1	amyloliquefaciens					139:155	Bacillus amyloliquefaciens	130:155	Bacillus amyloliquefaciens	130:155	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	0	59	with	fermentation	83:94	arg1	rhamnoides					115:124	Lactobacillus rhamnoides	101:124	Lactobacillus rhamnoides	101:124	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	6	60	theme	Scanning	1544:1551	arg1	SEM					1574:1576	SEM	1574:1576	SEM	1574:1576	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	6	60	theme	Scanning	1544:1551	arg1	microscope					1562:1571	Scanning electron microscope	1544:1571	Scanning electron microscope (SEM)	1544:1577	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	3	61	theme	rhamnoides	752:761	arg1	broth					729:733	fermentation broth	716:733	fermentation broth of Lactobacillus rhamnoides	716:761	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	62	theme	concentration	605:617	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	62	theme	concentration	605:617	arg1	%					620:620	glucose concentration 5%	597:620	glucose concentration 5%	597:620	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	8	63	theme	shells	1778:1783	arg1	use					1755:1757	full use	1750:1757	full use of shrimp and crab shells resources	1750:1793	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	5	64	theme	initial	1012:1018	arg1	pH 6.5					1020:1025	initial pH 6.5	1012:1025	initial pH 6.5	1012:1025	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	6	65	theme	CP/MAS-NMR	1520:1529	arg1	spectra					1531:1537	Solid-state 13C CP/MAS-NMR spectra	1504:1537	Solid-state 13C CP/MAS-NMR spectra	1504:1537	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	3	66	theme	cultured	623:630	arg1	48 h					637:640	cultured time 48 h	623:640	cultured time 48 h	623:640	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	66	theme	cultured	623:630	arg1	%					550:550	inoculum level 4%	534:550	inoculum level 4%	534:550	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	4	67	theme	final	871:875	arg1	residue					877:883	the final residue	867:883	the final residue	867:883	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	6	68	theme	Solid-state	1504:1514	arg1	spectra					1531:1537	Solid-state 13C CP/MAS-NMR spectra	1504:1537	Solid-state 13C CP/MAS-NMR spectra	1504:1537	In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM).					
31954122	8	69	theme	amino	1800:1804	arg1	composition					1811:1821	the amino acid composition	1796:1821	the amino acid composition of fermentation broth	1796:1843	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	5	70	theme	level	1002:1006	arg1	%					1009:1009	inoculum level 6%	993:1009	inoculum level 6%	993:1009	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	70	theme	level	1002:1006	arg1	conditions					969:978	the optimal cultured conditions	948:978	the optimal cultured conditions of BA01	948:986	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	0	71	from	shrimp	23:28	arg1	extraction					7:16	Chitin extraction	0:16	Chitin extraction from shrimp (Litopenaeus vannamei)	0:51	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	5	72	theme	protease	1132:1139	arg1	701.3 U/mL					1176:1185	701.3 U/mL	1176:1185	701.3 U/mL	1176:1185	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	72	theme	protease	1132:1139	arg1	activity					1141:1148	the protease activity	1128:1148	the protease activity of fermentation broth	1128:1170	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	73	theme	broth	1166:1170	arg1	701.3 U/mL					1176:1185	701.3 U/mL	1176:1185	701.3 U/mL	1176:1185	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	73	theme	broth	1166:1170	arg1	activity					1141:1148	the protease activity	1128:1148	the protease activity of fermentation broth	1128:1170	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	0	74	theme	two-step	74:81	arg1	fermentation					83:94	successive two-step fermentation	63:94	successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens	63:155	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	4	75	theme	lactic	925:930	arg1	acid					916:919	the main organic acid	899:919	the main organic acid	899:919	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	4	75	theme	lactic	925:930	arg1	acid					932:935	lactic acid	925:935	lactic acid	925:935	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	8	76	theme	fermentation	1826:1837	arg1	composition					1811:1821	the amino acid composition	1796:1821	the amino acid composition of fermentation broth	1796:1843	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	5	77	theme	concentration	1064:1076	arg1	%					1079:1079	glucose concentration 4%	1056:1079	glucose concentration 4%	1056:1079	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	4	78	theme	main	903:906	arg1	acid					916:919	the main organic acid	899:919	the main organic acid	899:919	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	4	78	theme	main	903:906	arg1	acid					932:935	lactic acid	925:935	lactic acid	925:935	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	5	79	theme	temperature	1037:1047	arg1	37 °C					1049:1053	cultured temperature 37 °C	1028:1053	cultured temperature 37 °C	1028:1053	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	9	80	from	nutrient	1963:1970	arg1	feed					1982:1985	animal feed	1975:1985	animal feed	1975:1985	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	5	81	theme	time	1095:1098	arg1	84 h.					1100:1104	cultured time 84 h.	1086:1104	cultured time 84 h.	1086:1104	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	2	82	theme	two-step	446:453	arg1	fermentation					455:466	two-step fermentation	446:466	two-step fermentation	446:466	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	5	83	theme	optimal	952:958	arg1	%					1009:1009	inoculum level 6%	993:1009	inoculum level 6%	993:1009	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	5	83	theme	optimal	952:958	arg1	conditions					969:978	the optimal cultured conditions	948:978	the optimal cultured conditions of BA01	948:986	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
31954122	8	84	dep	shrimp	1762:1767	arg1	resources					1785:1793	resources	1785:1793	resources	1785:1793	Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected.					
31954122	9	85	theme	high	1910:1913	arg1	value					1927:1931	high nutritional value	1910:1931	high nutritional value	1910:1931	The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.					
31954122	2	86	theme	optimal	410:416	arg1	conditions					431:440	the optimal fermentation conditions	406:440	the optimal fermentation conditions for two-step fermentation	406:466	The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established.					
31954122	7	87	theme	chitin	1715:1720	arg1	properties					1690:1699	the excellent physicochemical and structural properties	1645:1699	the excellent physicochemical and structural properties of commercial chitin	1645:1720	The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin.					
31954122	0	88	dep	shrimp	23:28	arg1	vannamei					43:50	Litopenaeus vannamei	31:50	Litopenaeus vannamei	31:50	Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens.					
31954122	3	89	theme	rhamnoides	660:669	arg1	conditions					522:531	the cultured conditions	509:531	the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides	509:669	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	89	theme	rhamnoides	660:669	arg1	types					707:711	types	707:711	types	707:711	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	3	89	theme	rhamnoides	660:669	arg1	quantities					692:701	the organic acid quantities	675:701	the organic acid quantities	675:701	Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides.					
31954122	7	90	theme	structural	1679:1688	arg1	properties					1690:1699	the excellent physicochemical and structural properties	1645:1699	the excellent physicochemical and structural properties of commercial chitin	1645:1720	The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin.					
31954122	4	91	theme	fermentation	796:807	arg1	3.4					819:821	3.4	819:821	3.4	819:821	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	4	91	theme	fermentation	796:807	arg1	pH					790:791	the pH	786:791	the pH of fermentation broth	786:813	Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid.					
31954122	5	92	theme	fermentation	1153:1164	arg1	broth					1166:1170	fermentation broth	1153:1170	fermentation broth	1153:1170	Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%.					
33143222	0	0	theme	Cell	62:65	arg1	Fate					67:70	the Cell Fate	58:70	the Cell Fate	58:70	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	1	theme	LM2	749:751	arg1	epitopes					760:767	LM2 (AGPs) epitopes	749:767	LM2 (AGPs) epitopes	749:767	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	1	theme	LM2	749:751	arg1	markers					782:788	positive markers	773:788	positive markers	773:788	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	2	theme	obtained	684:691	arg1	results					693:699	the obtained results	680:699	the obtained results	680:699	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	3	3	theme	wall	608:611	arg1	components					613:622	wall components	608:622	wall components	608:622	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	4	4	dep	epitopes	838:845	arg1	markers					860:866	negative markers	851:866	negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs)	851:1285	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	2	5	from	changes	408:414	arg1	reprogramming					424:436	cell reprogramming	419:436	cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate	419:533	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	1	6	theme	cell	199:202	arg1	walls					204:208	the cell walls	195:208	the cell walls	195:208	Changes in the composition of the cell walls are postulated to accompany changes in the cell's fate.					
33143222	4	7	dep	LM8	1330:1332	arg1	pectic					1335:1340	pectic	1335:1340	pectic	1335:1340	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	3	8	theme	components	613:622	arg1	immunolocalization					586:603	spatio-temporal immunolocalization	570:603	spatio-temporal immunolocalization of wall components	570:622	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	4	9	theme	AGPs	754:757	arg1	epitopes					760:767	LM2 (AGPs) epitopes	749:767	LM2 (AGPs) epitopes	749:767	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	9	theme	AGPs	754:757	arg1	markers					782:788	positive markers	773:788	positive markers	773:788	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	10	theme	positive	773:780	arg1	epitopes					760:767	LM2 (AGPs) epitopes	749:767	LM2 (AGPs) epitopes	749:767	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	10	theme	positive	773:780	arg1	markers					782:788	positive markers	773:788	positive markers	773:788	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	11	theme	cells	871:875	arg1	markers					860:866	negative markers	851:866	negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs)	851:1285	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	1	12	theme	walls	204:208	arg1	composition					180:190	the composition	176:190	the composition of the cell walls	176:208	Changes in the composition of the cell walls are postulated to accompany changes in the cell's fate.					
33143222	0	13	theme	Fate	67:70	arg1	Reprogramming					41:53	the Reprogramming	37:53	the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	37:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	3	14	theme	somatic	648:654	arg1	embryogenesis					656:668	somatic embryogenesis	648:668	somatic embryogenesis	648:668	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	0	15	theme	Somatic	130:136	arg1	Embryogenesis					138:150	Somatic Embryogenesis	130:150	Somatic Embryogenesis	130:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	16	theme	positive	1010:1017	arg1	markers					1019:1025	positive markers	1010:1025	positive markers	1010:1025	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	16	theme	positive	1010:1017	arg1	AGPs					970:973	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	AGPs	970:973	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	16	theme	positive	1010:1017	arg1	epitopes					997:1004	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	epitopes	997:1004	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	17	theme	xylem	1232:1236	arg1	pole					1238:1241	the xylem pole	1228:1241	the xylem pole	1228:1241	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	18	theme	LM8	938:940	arg1	markers					1019:1025	positive markers	1010:1025	positive markers	1010:1025	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	18	theme	LM8	938:940	arg1	AGPs					970:973	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	AGPs	970:973	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	18	theme	LM8	938:940	arg1	epitopes					997:1004	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	epitopes	997:1004	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	3	19	theme	embryogenesis	656:668	arg1	induction					635:643	the induction	631:643	the induction of somatic embryogenesis	631:668	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	4	20	theme	negative	1056:1063	arg1	marker					1065:1070	negative marker	1056:1070	negative marker	1056:1070	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	21	theme	constitutive	1413:1424	arg1	components					1431:1440	not constitutive wall components	1409:1440	not constitutive wall components	1409:1440	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	2	22	theme	cell	419:422	arg1	reprogramming					424:436	cell reprogramming	419:436	cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate	419:533	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	0	23	from	Marker	27:32	arg1	Example					79:85	the Example	75:85	the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	75:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	1	24	from	changes	238:244	arg1	fate					260:263	the cell's fate	249:263	the cell's fate	249:263	Changes in the composition of the cell walls are postulated to accompany changes in the cell's fate.					
33143222	4	25	dep	state	923:927	arg1	markers					1019:1025	positive markers	1010:1025	positive markers	1010:1025	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	25	dep	state	923:927	arg1	AGPs					970:973	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	AGPs	970:973	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	25	dep	state	923:927	arg1	marker					1065:1070	negative marker	1056:1070	negative marker	1056:1070	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	25	dep	state	923:927	arg1	epitopes					997:1004	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	epitopes	997:1004	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	26	theme	pectic	943:948	arg1	markers					1019:1025	positive markers	1010:1025	positive markers	1010:1025	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	26	theme	pectic	943:948	arg1	AGPs					970:973	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	AGPs	970:973	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	26	theme	pectic	943:948	arg1	epitopes					997:1004	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	epitopes	997:1004	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	0	27	theme	Wall	5:8	arg1	Composition					10:20	Cell Wall Composition	0:20	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	0:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	2	28	theme	cell	525:528	arg1	fate					530:533	cell fate	525:533	cell fate	525:533	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	0	29	theme	Cell	0:3	arg1	Composition					10:20	Cell Wall Composition	0:20	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	0:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	0	30	from	Embryogenesis	138:150	arg1	Example					79:85	the Example	75:85	the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	75:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	31	theme	JIM11	980:984	arg1	markers					1019:1025	positive markers	1010:1025	positive markers	1010:1025	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	31	theme	JIM11	980:984	arg1	extensin					987:994	extensin	987:994	extensin	987:994	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	31	theme	JIM11	980:984	arg1	AGPs					970:973	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	AGPs	970:973	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	31	theme	JIM11	980:984	arg1	epitopes					997:1004	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	epitopes	997:1004	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	32	theme	pericycle	1209:1217	arg1	cells					1219:1223	pericycle cells	1209:1223	pericycle cells	1209:1223	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	2	33	theme	proteins	364:371	arg1	presence					319:326	the presence	315:326	the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes	315:402	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	2	34	dep	pectic	340:345	arg1	epitopes					395:402	epitopes	395:402	epitopes	395:402	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	4	35	theme	wall	1426:1429	arg1	components					1431:1440	not constitutive wall components	1409:1440	not constitutive wall components	1409:1440	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	2	36	theme	arabinogalactan	348:362	arg1	AGPs					374:377	AGPs	374:377	AGPs	374:377	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	2	36	theme	arabinogalactan	348:362	arg1	proteins					364:371	arabinogalactan proteins	348:371	arabinogalactan proteins (AGPs)	348:378	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	3	37	used	used	561:564	arg2	antibodies					545:554	Selected antibodies	536:554	Selected antibodies	536:554	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	4	38	dep	LM19	804:807	arg1	pectic					810:815	pectic	810:815	pectic	810:815	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	39	theme	LM6	1032:1034	arg1	epitope					1045:1051	LM6 (pectic) epitope	1032:1051	LM6 (pectic) epitope	1032:1051	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	2	40	theme	pectic	340:345	arg1	presence					319:326	the presence	315:326	the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes	315:402	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	2	41	theme	extensins	385:393	arg1	presence					319:326	the presence	315:326	the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes	315:402	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	4	42	dep	contribute	1468:1477	arg1	be					709:710	be	709:710	can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming	705:1503	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	0	43	theme	carota	99:104	arg1	Hypocotyl					111:119	a Daucus carota (L.) Hypocotyl	90:119	a Daucus carota (L.) Hypocotyl	90:119	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	44	dep	AGPs	970:973	arg1	JIM8					952:955	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	JIM8	952:955	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	44	dep	AGPs	970:973	arg1	LM2					965:967	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	LM2	965:967	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	44	dep	AGPs	970:973	arg1	JIM13					958:962	the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes	934:1004	JIM13	958:962	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	3	45	theme	Selected	536:543	arg1	antibodies					545:554	Selected antibodies	536:554	Selected antibodies	536:554	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	4	46	theme	negative	851:858	arg1	markers					860:866	negative markers	851:866	negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs)	851:1285	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	47	theme	cell	1486:1489	arg1	reprogramming					1491:1503	the cell reprogramming	1482:1503	the cell reprogramming	1482:1503	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	48	dep	LM20	1254:1257	arg1	pectic					1260:1265	pectic	1260:1265	pectic	1260:1265	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	49	theme	wall	1304:1307	arg1	components					1309:1318	constitutive wall components	1291:1318	constitutive wall components	1291:1318	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	49	theme	wall	1304:1307	arg1	epitopes					838:845	the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes	795:845	the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs)	795:1285	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	0	50	from	Reprogramming	41:53	arg1	Example					79:85	the Example	75:85	the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	75:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	1	51	from	Changes	165:171	arg1	composition					180:190	the composition	176:190	the composition of the cell walls	176:208	Changes in the composition of the cell walls are postulated to accompany changes in the cell's fate.					
33143222	4	52	theme	negative	1188:1195	arg1	markers					1197:1203	negative markers	1188:1203	negative markers for pericycle cells	1188:1223	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	53	theme	cells	1075:1079	arg1	marker					1065:1070	negative marker	1056:1070	negative marker	1056:1070	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	54	theme	constitutive	1291:1302	arg1	components					1309:1318	constitutive wall components	1291:1318	constitutive wall components	1291:1318	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	54	theme	constitutive	1291:1302	arg1	epitopes					838:845	the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes	795:845	the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs)	795:1285	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	0	55	from	Example	79:85	arg1	Marker					27:32	a Marker	25:32	a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	25:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	2	56	theme	fate	530:533	arg1	changes					514:520	changes	514:520	changes of cell fate	514:533	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	4	57	dep	LM5	1146:1148	arg1	markers					1197:1203	negative markers	1188:1203	negative markers for pericycle cells	1188:1223	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	57	dep	LM5	1146:1148	arg1	pectic					1151:1156	pectic	1151:1156	pectic	1151:1156	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	58	theme	pectic	1037:1042	arg1	epitope					1045:1051	LM6 (pectic) epitope	1032:1051	LM6 (pectic) epitope	1032:1051	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	2	59	from	relationship	294:305	arg1	reprogramming					424:436	cell reprogramming	419:436	cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate	419:533	We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate.					
33143222	4	60	theme	positive	1125:1132	arg1	marker					1134:1139	a positive marker	1123:1139	a positive marker	1123:1139	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	0	61	theme	Hypocotyl	111:119	arg1	Example					79:85	the Example	75:85	the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	75:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	62	from	markers	1197:1203	arg1	pole					1238:1241	the xylem pole	1228:1241	the xylem pole	1228:1241	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	63	theme	state	923:927	arg1	JIM4					1108:1111	the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4	894:1111	the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs)	894:1118	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	63	theme	state	923:927	arg1	AGPs					1114:1117	AGPs	1114:1117	AGPs	1114:1117	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	64	dep	markers	1019:1025	arg1	2					931:931	2	931:931	2	931:931	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	3	65	theme	spatio-temporal	570:584	arg1	immunolocalization					586:603	spatio-temporal immunolocalization	570:603	spatio-temporal immunolocalization of wall components	570:622	Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis.					
33143222	0	66	dep	carota	99:104	arg1	L.					107:108	L.	107:108	L.	107:108	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	67	theme	meristematic/pluripotent	898:921	arg1	state					923:927	the meristematic/pluripotent state	894:927	the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs)	894:1118	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	0	68	theme	Reprogramming	41:53	arg1	Marker					27:32	a Marker	25:32	a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis	25:150	Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced.					
33143222	4	69	dep	LM6	735:737	arg1	pectic					740:745	pectic	740:745	pectic	740:745	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	69	dep	LM6	735:737	arg1	1					728:728	1	728:728	1	728:728	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	69	dep	LM6	735:737	arg1	components					1431:1440	not constitutive wall components	1409:1440	not constitutive wall components	1409:1440	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	69	dep	LM6	735:737	arg1	extensins					1451:1459	the extensins	1447:1459	(1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins	727:1459	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	70	theme	LM5	799:801	arg1	components					1309:1318	constitutive wall components	1291:1318	constitutive wall components	1291:1318	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
33143222	4	70	theme	LM5	799:801	arg1	epitopes					838:845	the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes	795:845	the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs)	795:1285	Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.					
34072325	3	0	theme	chemical	465:472	arg1	analysis					474:481	The chemical analysis	461:481	The chemical analysis	461:481	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	5	1	theme	mineral	971:977	arg1	content					979:985	a high mineral content	964:985	a high mineral content	964:985	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	1	2	theme	minerals	209:216	arg1	source					199:204	a rich source	192:204	a rich source of minerals, carbohydrates and antioxidants	192:248	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	2	3	theme	offshore	444:451	arg1	waters					453:458	Malaysian wild offshore waters	429:458	Malaysian wild offshore waters	429:458	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	3	4	dep	toxic	594:598	arg1	Cd					601:602	Cd	601:602	Cd	601:602	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	4	dep	toxic	594:598	arg1	Hg					609:610	Hg	609:610	Hg	609:610	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	4	dep	toxic	594:598	arg1	Pb					605:606	Pb	605:606	Pb	605:606	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	7	5	theme	t-carrageenans	1126:1139	arg1	mixture					1097:1103	a mixture	1095:1103	a mixture of prevailing κ- and t-carrageenans	1095:1139	Both samples showed a mixture of prevailing κ- and t-carrageenans.					
34072325	9	6	theme	favorable	1362:1370	arg1	features					1397:1404	its favorable chemical and nutritional features	1358:1404	its favorable chemical and nutritional features	1358:1404	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
34072325	5	7	theme	K.	932:933	arg1	striatus					935:942	K. striatus	932:942	K. striatus	932:942	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	6	8	from	differences	1003:1013	arg1	concentrations					1024:1037	metal concentrations	1018:1037	metal concentrations	1018:1037	No significant differences in metal concentrations were found between the two species.					
34072325	3	9	theme	coupled	513:519	arg1	spectroscopy					545:556	inductively coupled plasma-optical emission spectroscopy	501:556	inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se)	501:663	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	1	10	theme	carbohydrates	219:231	arg1	source					199:204	a rich source	192:204	a rich source of minerals, carbohydrates and antioxidants	192:248	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	4	11	theme	antioxidant	765:775	arg1	capacities					777:786	the soluble and fat-soluble antioxidant capacities	737:786	the soluble and fat-soluble antioxidant capacities	737:786	Furthermore, the soluble and fat-soluble antioxidant capacities were determined by FRAP, DPPH and ABTS assays.					
34072325	5	12	theme	higher	868:873	arg1	content					875:881	a higher content	866:881	a higher content of trace elements in K. alvarezii	866:915	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	3	13	theme	plasma-optical	521:534	arg1	spectroscopy					545:556	inductively coupled plasma-optical emission spectroscopy	501:556	inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se)	501:663	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	14	dep	elements	631:638	arg1	Mn					641:642	Mn	641:642	Mn	641:642	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	6	15	theme	metal	1018:1022	arg1	concentrations					1024:1037	metal concentrations	1018:1037	metal concentrations	1018:1037	No significant differences in metal concentrations were found between the two species.					
34072325	4	16	theme	soluble	741:747	arg1	capacities					777:786	the soluble and fat-soluble antioxidant capacities	737:786	the soluble and fat-soluble antioxidant capacities	737:786	Furthermore, the soluble and fat-soluble antioxidant capacities were determined by FRAP, DPPH and ABTS assays.					
34072325	3	17	theme	carrageenans	696:707	arg1	investigation					709:721	carrageenans investigation	696:721	carrageenans investigation	696:721	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	9	18	used	used	1313:1316	arg2	alvarezii					1294:1302	K. alvarezii	1291:1302	K. alvarezii	1291:1302	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
34072325	9	18	used	used	1313:1316	arg2	additive					1338:1345	additive	1338:1345	additive	1338:1345	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
34072325	1	19	theme	valid	141:145	arg1	use					83:85	The use	79:85	The use of seaweeds as additives in animal nutrition	79:130	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	1	19	theme	valid	141:145	arg1	option					147:152	a valid option	139:152	a valid option to traditional feed	139:172	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	2	20	dep	in	317:318	arg1	vitro					320:324	vitro	320:324	vitro	320:324	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	8	21	dep	K.	1252:1253	arg1	striatus					1255:1262	K. striatus	1252:1262	K. striatus	1252:1262	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	4	22	theme	fat-soluble	753:763	arg1	capacities					777:786	the soluble and fat-soluble antioxidant capacities	737:786	the soluble and fat-soluble antioxidant capacities	737:786	Furthermore, the soluble and fat-soluble antioxidant capacities were determined by FRAP, DPPH and ABTS assays.					
34072325	2	23	from	composition	301:311	arg1	waters					453:458	Malaysian wild offshore waters	429:458	Malaysian wild offshore waters	429:458	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	1	24	theme	antioxidants	237:248	arg1	source					199:204	a rich source	192:204	a rich source of minerals, carbohydrates and antioxidants	192:248	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	2	25	theme	study	267:271	arg1	aim					255:257	The aim	251:257	The aim of this study	251:271	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	2	26	theme	antioxidant	326:336	arg1	capacity					338:345	in vitro antioxidant capacity	317:345	in vitro antioxidant capacity	317:345	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	0	27	theme	Eucheumatoids	22:34	arg1	Chemistry					0:8	Chemistry	0:8	Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications.	0:77	Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications.					
34072325	3	28	dep	Mn	641:642	arg1	Cu					649:650	Cu	649:650	Cu	649:650	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	28	dep	Mn	641:642	arg1	Fe					645:646	Fe	645:646	Fe	645:646	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	28	dep	Mn	641:642	arg1	Zn					657:658	Zn	657:658	Zn	657:658	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	28	dep	Mn	641:642	arg1	Se					661:662	Se	661:662	Se	661:662	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	28	dep	Mn	641:642	arg1	Ni					653:654	Ni	653:654	Ni	653:654	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	4	29	theme	ABTS	822:825	arg1	assays					827:832	ABTS assays	822:832	ABTS assays	822:832	Furthermore, the soluble and fat-soluble antioxidant capacities were determined by FRAP, DPPH and ABTS assays.					
34072325	1	30	from	additives	102:110	arg1	nutrition					122:130	animal nutrition	115:130	animal nutrition	115:130	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	3	31	theme	emission	536:543	arg1	spectroscopy					545:556	inductively coupled plasma-optical emission spectroscopy	501:556	inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se)	501:663	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	0	32	theme	Tropical	13:20	arg1	Eucheumatoids					22:34	Tropical Eucheumatoids	13:34	Tropical Eucheumatoids	13:34	Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications.					
34072325	5	33	theme	chemical	839:846	arg1	analysis					848:855	The chemical analysis	835:855	The chemical analysis	835:855	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	1	34	theme	seaweeds	90:97	arg1	use					83:85	The use	79:85	The use of seaweeds as additives in animal nutrition	79:130	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	1	34	theme	seaweeds	90:97	arg1	option					147:152	a valid option	139:152	a valid option to traditional feed	139:172	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	3	35	dep	Cd	601:602	arg1	As					613:614	As	613:614	As	613:614	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	2	36	theme	wild	439:442	arg1	waters					453:458	Malaysian wild offshore waters	429:458	Malaysian wild offshore waters	429:458	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	9	37	theme	chemical	1372:1379	arg1	features					1397:1404	its favorable chemical and nutritional features	1358:1404	its favorable chemical and nutritional features	1358:1404	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
34072325	0	38	dep	Food	51:54	arg1	Applications					65:76	Applications	65:76	Applications	65:76	Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications.					
34072325	1	39	theme	traditional	157:167	arg1	feed					169:172	traditional feed	157:172	traditional feed	157:172	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	2	40	theme	eucheumatoids	363:375	arg1	capacity					338:345	in vitro antioxidant capacity	317:345	in vitro antioxidant capacity	317:345	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	2	40	theme	eucheumatoids	363:375	arg1	composition					301:311	the chemical composition	288:311	the chemical composition	288:311	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	2	41	theme	Malaysian	429:437	arg1	waters					453:458	Malaysian wild offshore waters	429:458	Malaysian wild offshore waters	429:458	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	3	42	theme	NMR	666:668	arg1	spectroscopy					670:681	NMR spectroscopy	666:681	NMR spectroscopy	666:681	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	3	43	theme	toxic	594:598	arg1	concentration					577:589	the concentration	573:589	the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se)	573:663	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	2	44	from	capacity	338:345	arg1	waters					453:458	Malaysian wild offshore waters	429:458	Malaysian wild offshore waters	429:458	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	2	45	theme	tropical	354:361	arg1	eucheumatoids					363:375	two tropical eucheumatoids	350:375	two tropical eucheumatoids	350:375	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	2	45	theme	tropical	354:361	arg1	alvarezii					390:398	Kappaphycus alvarezii	378:398	Kappaphycus alvarezii	378:398	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	2	45	theme	tropical	354:361	arg1	striatus					416:423	Kappaphycus striatus	404:423	Kappaphycus striatus	404:423	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	5	46	theme	high	966:969	arg1	content					979:985	a high mineral content	964:985	a high mineral content	964:985	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	2	47	theme	chemical	292:299	arg1	composition					301:311	the chemical composition	288:311	the chemical composition	288:311	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	9	48	theme	nutritional	1385:1395	arg1	features					1397:1404	its favorable chemical and nutritional features	1358:1404	its favorable chemical and nutritional features	1358:1404	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
34072325	3	49	theme	essential	621:629	arg1	elements					631:638	essential elements	621:638	essential elements (Mn, Fe, Cu, Ni, Zn, Se)	621:663	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	8	50	theme	soluble	1165:1171	arg1	antioxidants					1189:1200	soluble and fat-soluble antioxidants	1165:1200	soluble and fat-soluble antioxidants	1165:1200	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	5	51	theme	K.	904:905	arg1	alvarezii					907:915	K. alvarezii	904:915	K. alvarezii	904:915	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	3	52	theme	elements	631:638	arg1	concentration					577:589	the concentration	573:589	the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se)	573:663	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	5	53	theme	trace	886:890	arg1	elements					892:899	trace elements	886:899	trace elements	886:899	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	8	54	theme	antioxidants	1189:1200	arg1	levels					1155:1160	the levels	1151:1160	the levels of soluble and fat-soluble antioxidants in K. alvarezii	1151:1216	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	8	54	theme	antioxidants	1189:1200	arg1	higher					1237:1242	higher	1237:1242	higher	1237:1242	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	6	55	theme	significant	991:1001	arg1	differences					1003:1013	No significant differences	988:1013	No significant differences in metal concentrations	988:1037	No significant differences in metal concentrations were found between the two species.					
34072325	5	56	theme	elements	892:899	arg1	content					875:881	a higher content	866:881	a higher content of trace elements in K. alvarezii	866:915	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	1	57	theme	animal	115:120	arg1	nutrition					122:130	animal nutrition	115:130	animal nutrition	115:130	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	1	58	theme	rich	194:197	arg1	source					199:204	a rich source	192:204	a rich source of minerals, carbohydrates and antioxidants	192:248	The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants.					
34072325	7	59	theme	prevailing	1108:1117	arg1	κ-					1119:1120	κ-	1119:1120	κ-	1119:1120	Both samples showed a mixture of prevailing κ- and t-carrageenans.					
34072325	8	60	from	levels	1155:1160	arg1	alvarezii					1208:1216	K. alvarezii	1205:1216	K. alvarezii	1205:1216	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	8	61	theme	K.	1205:1206	arg1	alvarezii					1208:1216	K. alvarezii	1205:1216	K. alvarezii	1205:1216	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	8	62	theme	fat-soluble	1177:1187	arg1	antioxidants					1189:1200	soluble and fat-soluble antioxidants	1165:1200	soluble and fat-soluble antioxidants	1165:1200	Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus.					
34072325	7	63	theme	κ-	1119:1120	arg1	mixture					1097:1103	a mixture	1095:1103	a mixture of prevailing κ- and t-carrageenans	1095:1139	Both samples showed a mixture of prevailing κ- and t-carrageenans.					
34072325	3	64	used	used	687:690	arg2	spectroscopy					670:681	NMR spectroscopy	666:681	NMR spectroscopy	666:681	The chemical analysis was performed via inductively coupled plasma-optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation.					
34072325	0	65	dep	Chemistry	0:8	arg1	Potential					37:45	Potential	37:45	Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications.	0:77	Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications.					
34072325	2	66	theme	in	317:318	arg1	capacity					338:345	in vitro antioxidant capacity	317:345	in vitro antioxidant capacity	317:345	The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters.					
34072325	5	67	from	content	875:881	arg1	alvarezii					907:915	K. alvarezii	904:915	K. alvarezii	904:915	The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content.					
34072325	9	68	theme	K.	1291:1292	arg1	additive					1338:1345	additive	1338:1345	additive	1338:1345	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
34072325	9	68	theme	K.	1291:1292	arg1	alvarezii					1294:1302	K. alvarezii	1291:1302	K. alvarezii	1291:1302	Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.					
33549877	2	0	theme	hydrolysis	424:433	arg1	process					435:441	ELEBs hydrolysis process	418:441	ELEBs hydrolysis process for extraction of glucose	418:467	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	4	1	theme	ELEBs	902:906	arg1	morphology					863:872	morphology	863:872	morphology	863:872	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	4	1	theme	ELEBs	902:906	arg1	composition					887:897	chemical composition	878:897	chemical composition	878:897	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	8	2	contain	has	1474:1476	arg1	strategy					1465:1472	the developed strategy	1451:1472	the developed strategy	1451:1472	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	8	2	contain	has	1474:1476	arg2	potential					1486:1494	a great potential	1478:1494	a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications	1478:1601	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	2	3	theme	cellulase	309:317	arg1	production					319:328	cellulase production	309:328	cellulase production via submerged fungal fermentation (SFF)	309:368	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	4	4	theme	Dispersive	967:976	arg1	Spectrometry					978:989	Energy Dispersive Spectrometry	960:989	Energy Dispersive Spectrometry	960:989	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	2	5	theme	ELEBs	418:422	arg1	process					435:441	ELEBs hydrolysis process	418:441	ELEBs hydrolysis process for extraction of glucose	418:467	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	6	used	used	410:413	arg2	s					408:408	the resultant fungal cellulase w s	375:408	the resultant fungal cellulase w s	375:408	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	3	7	theme	cotton	740:745	arg1	substrate					747:755	cotton substrate	740:755	cotton substrate	740:755	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	8	dep	cellulose	709:717	arg1	element					729:735	the main element	720:735	the main element in cotton substrate	720:755	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	9	theme	acid	618:621	arg1	leaching					623:630	acid leaching	618:630	acid leaching	618:630	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	8	10	theme	glucose	1527:1533	arg1	product					1535:1541	a glucose product	1525:1541	a glucose product that could be used in biofuels and bioplastics applications	1525:1601	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	7	11	theme	glucose	1370:1376	arg1	recovery					1378:1385	glucose recovery	1370:1385	glucose recovery	1370:1385	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	3	12	theme	degradation	778:788	arg1	rate					790:793	the degradation rate	774:793	the degradation rate	774:793	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	13	theme	main	724:727	arg1	element					729:735	the main element	720:735	the main element in cotton substrate	720:755	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	2	14	theme	glucose	461:467	arg1	extraction					447:456	extraction	447:456	extraction of glucose	447:467	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	6	15	theme	ELEBs	1195:1199	arg1	hydrolysis					1201:1210	ELEBs hydrolysis	1195:1210	ELEBs hydrolysis	1195:1210	The resultant cellulase was upscaled in a bioreactor and used in ELEBs hydrolysis.					
33549877	7	16	dep	activity	1357:1364	arg1	the					1343:1345	the	1343:1345	the	1343:1345	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	8	17	theme	bioplastics	1578:1588	arg1	applications					1590:1601	bioplastics applications	1578:1601	bioplastics applications	1578:1601	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	3	18	dep	types	534:538	arg1	decrease					679:686	decrease	679:686	to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process	676:825	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	18	dep	types	534:538	arg1	remove					635:640	remove	635:640	to remove any contamination (e.g. dyes)	632:670	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	6	19	theme	resultant	1134:1142	arg1	cellulase					1144:1152	The resultant cellulase	1130:1152	The resultant cellulase	1130:1152	The resultant cellulase was upscaled in a bioreactor and used in ELEBs hydrolysis.					
33549877	0	20	theme	glucose	43:49	arg1	recovery					31:38	recovery	31:38	recovery of glucose from end-of-life euro banknotes	31:81	Sustainable green strategy for recovery of glucose from end-of-life euro banknotes.					
33549877	5	21	theme	cellulase	1042:1050	arg1	production					1052:1061	cellulase production	1042:1061	cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g)	1042:1127	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	2	22	used	used	283:286	arg2	substrates					267:276	the substrates	263:276	the substrates	263:276	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	4	23	theme	Electron	936:943	arg1	Microscope					945:954	Scanning Electron Microscope	927:954	Scanning Electron Microscope	927:954	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	7	24	theme	pretreatment	1260:1271	arg1	methods					1273:1279	the optimized pretreatment methods	1246:1279	the optimized pretreatment methods (milling followed by leaching process)	1246:1318	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	2	25	theme	cellulase	396:404	arg1	s					408:408	the resultant fungal cellulase w s	375:408	the resultant fungal cellulase w s	375:408	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	3	26	theme	fermentation	806:817	arg1	process					819:825	the fermentation process	802:825	the fermentation process	802:825	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	2	27	theme	fungal	344:349	arg1	fermentation					351:362	submerged fungal fermentation	334:362	submerged fungal fermentation (SFF)	334:368	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	27	theme	fungal	344:349	arg1	SFF					365:367	SFF	365:367	SFF	365:367	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	28	theme	fungal	389:394	arg1	s					408:408	the resultant fungal cellulase w s	375:408	the resultant fungal cellulase w s	375:408	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	7	29	theme	cellulase	1347:1355	arg1	activity					1357:1364	cellulase activity	1347:1364	cellulase activity	1347:1364	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	2	30	theme	submerged	334:342	arg1	fermentation					351:362	submerged fungal fermentation	334:362	submerged fungal fermentation (SFF)	334:368	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	30	theme	submerged	334:342	arg1	SFF					365:367	SFF	365:367	SFF	365:367	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	31	theme	resultant	379:387	arg1	s					408:408	the resultant fungal cellulase w s	375:408	the resultant fungal cellulase w s	375:408	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	7	32	theme	leaching	1302:1309	arg1	process					1311:1317	leaching process	1302:1317	leaching process	1302:1317	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	1	33	theme	Euro	93:96	arg1	banknotes					98:106	Euro banknotes	93:106	Euro banknotes	93:106	Usually, Euro banknotes are made from cotton substrates and their waste is disposed of in landfill or is incinerated.					
33549877	7	34	dep	methods	1273:1279	arg1	milling					1282:1288	milling	1282:1288	milling followed by leaching process	1282:1317	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	3	35	theme	e.g.	661:664	arg1	contamination					646:658	any contamination	642:658	any contamination (e.g. dyes)	642:670	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	35	theme	e.g.	661:664	arg1	dyes					666:669	e.g. dyes	661:669	e.g. dyes	661:669	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	36	from	element	729:735	arg1	substrate					747:755	cotton substrate	740:755	cotton substrate	740:755	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	8	37	theme	great	1480:1484	arg1	potential					1486:1494	a great potential	1478:1494	a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications	1478:1601	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	4	38	theme	pretreatments	842:854	arg1	effect					832:837	The effect	828:837	The effect of pretreatments on the morphology and chemical composition of ELEBs	828:906	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	0	39	from	banknotes	73:81	arg1	recovery					31:38	recovery	31:38	recovery of glucose from end-of-life euro banknotes	31:81	Sustainable green strategy for recovery of glucose from end-of-life euro banknotes.					
33549877	5	40	from	ELEBs	1080:1084	arg1	production					1052:1061	cellulase production	1042:1061	cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g)	1042:1127	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	3	41	theme	milling	568:574	arg1	process					576:582	milling process	568:582	milling process	568:582	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	4	42	from	effect	832:837	arg1	morphology					863:872	morphology	863:872	morphology	863:872	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	4	42	from	effect	832:837	arg1	composition					887:897	chemical composition	878:897	chemical composition	878:897	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	4	43	theme	Energy	960:965	arg1	Spectrometry					978:989	Energy Dispersive Spectrometry	960:989	Energy Dispersive Spectrometry	960:989	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	4	44	theme	Scanning	927:934	arg1	Microscope					945:954	Scanning Electron Microscope	927:954	Scanning Electron Microscope	927:954	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	5	45	theme	high	1091:1094	arg1	activity					1106:1113	high cellulase activity	1091:1113	high cellulase activity (12.97 FPU/g)	1091:1127	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	5	45	theme	high	1091:1094	arg1	FPU/g					1122:1126	12.97 FPU/g	1116:1126	12.97 FPU/g	1116:1126	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	4	46	dep	morphology	863:872	arg1	the					859:861	the	859:861	the	859:861	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	4	47	theme	chemical	878:885	arg1	composition					887:897	chemical composition	878:897	chemical composition	878:897	The effect of pretreatments on the morphology and chemical composition of ELEBs was observed using Scanning Electron Microscope and Energy Dispersive Spectrometry.					
33549877	8	48	theme	developed	1455:1463	arg1	strategy					1465:1472	the developed strategy	1451:1472	the developed strategy	1451:1472	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	5	49	theme	treated	1072:1078	arg1	ELEBs					1080:1084	the treated ELEBs	1068:1084	the treated ELEBs	1068:1084	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	2	50	theme	w	406:406	arg1	s					408:408	the resultant fungal cellulase w s	375:408	the resultant fungal cellulase w s	375:408	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	5	51	theme	cellulase	1096:1104	arg1	activity					1106:1113	high cellulase activity	1091:1113	high cellulase activity (12.97 FPU/g)	1091:1127	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	5	51	theme	cellulase	1096:1104	arg1	FPU/g					1122:1126	12.97 FPU/g	1116:1126	12.97 FPU/g	1116:1126	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	3	52	theme	different	524:532	arg1	types					534:538	different types	524:538	different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process	524:825	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	8	53	used	used	1557:1560	arg2	product					1535:1541	a glucose product	1525:1541	a glucose product that could be used in biofuels and bioplastics applications	1525:1601	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	7	54	theme	optimized	1250:1258	arg1	methods					1273:1279	the optimized pretreatment methods	1246:1279	the optimized pretreatment methods (milling followed by leaching process)	1246:1318	Finally, the results showed that the optimized pretreatment methods (milling followed by leaching process) significantly improved the cellulase activity and glucose recovery, which was estimated by 96%.					
33549877	2	55	theme	euro	239:242	arg1	ELEBs					255:259	ELEBs	255:259	ELEBs	255:259	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	55	theme	euro	239:242	arg1	banknotes					244:252	the end-of-life euro banknotes	223:252	the end-of-life euro banknotes (ELEBs)	223:260	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	5	56	with	production	1052:1061	arg1	activity					1106:1113	high cellulase activity	1091:1113	high cellulase activity (12.97 FPU/g)	1091:1127	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	5	56	with	production	1052:1061	arg1	FPU/g					1122:1126	12.97 FPU/g	1116:1126	12.97 FPU/g	1116:1126	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	3	57	theme	NaOH/urea	593:601	arg1	alkali					585:590	alkali	585:590	alkali (NaOH/urea solution)	585:611	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	3	57	theme	NaOH/urea	593:601	arg1	solution					603:610	NaOH/urea solution	593:610	NaOH/urea solution	593:610	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	2	58	theme	end-of-life	227:237	arg1	ELEBs					255:259	ELEBs	255:259	ELEBs	255:259	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	2	58	theme	end-of-life	227:237	arg1	banknotes					244:252	the end-of-life euro banknotes	223:252	the end-of-life euro banknotes (ELEBs)	223:260	In order to valorize the end-of-life euro banknotes (ELEBs), the substrates were used in this research for cellulase production via submerged fungal fermentation (SFF), and the resultant fungal cellulase w s used in ELEBs hydrolysis process for extraction of glucose.					
33549877	5	59	theme	Trichoderma	1004:1014	arg1	reesei-DSM76					1016:1027	Trichoderma reesei-DSM76	1004:1027	Trichoderma reesei-DSM76	1004:1027	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	1	60	theme	cotton	122:127	arg1	substrates					129:138	cotton substrates	122:138	cotton substrates	122:138	Usually, Euro banknotes are made from cotton substrates and their waste is disposed of in landfill or is incinerated.					
33549877	8	61	theme	ELEBs	1514:1518	arg1	conversion					1500:1509	conversion	1500:1509	conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications	1500:1601	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	0	62	theme	euro	68:71	arg1	banknotes					73:81	end-of-life euro banknotes	56:81	end-of-life euro banknotes	56:81	Sustainable green strategy for recovery of glucose from end-of-life euro banknotes.					
33549877	3	63	theme	pretreatments	543:555	arg1	types					534:538	different types	524:538	different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process	524:825	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33549877	6	64	used	used	1187:1190	arg2	cellulase					1144:1152	The resultant cellulase	1130:1152	The resultant cellulase	1130:1152	The resultant cellulase was upscaled in a bioreactor and used in ELEBs hydrolysis.					
33549877	0	65	theme	end-of-life	56:66	arg1	banknotes					73:81	end-of-life euro banknotes	56:81	end-of-life euro banknotes	56:81	Sustainable green strategy for recovery of glucose from end-of-life euro banknotes.					
33549877	5	66	used	used	1033:1036	arg2	reesei-DSM76					1016:1027	Trichoderma reesei-DSM76	1004:1027	Trichoderma reesei-DSM76	1004:1027	Afterwards, Trichoderma reesei-DSM76 was used for cellulase production from the treated ELEBs with high cellulase activity (12.97 FPU/g).					
33549877	8	67	theme	obtained	1433:1440	arg1	results					1442:1448	the obtained results	1429:1448	the obtained results	1429:1448	According to the obtained results, the developed strategy has a great potential for conversion of ELEBs into a glucose product that could be used in biofuels and bioplastics applications.					
33549877	3	68	theme	cellulose	709:717	arg1	crystallinity					692:704	the crystallinity	688:704	the crystallinity of cellulose (the main element in cotton substrate)	688:756	The experiments were started by exposing the ELEBs to different types of pretreatments, including milling process, alkali (NaOH/urea solution), and acid leaching to remove any contamination (e.g. dyes) and to decrease the crystallinity of cellulose (the main element in cotton substrate) thus increasing the degradation rate during the fermentation process.					
33456008	2	0	theme	various	378:384	arg1	mixes					386:390	various mixes	378:390	various mixes of these powders	378:407	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	6	1	theme	µ	834:834	arg1	k					836:836	the friction coefficient µ k	809:836	the friction coefficient µ k	809:836	It was observed that as the friction coefficient µ k was reduced, most subjects strongly felt the slippery feel.					
33456008	1	2	theme	preferred	167:175	arg1	Sara-sara					71:79	The Sara-sara feel	67:84	The Sara-sara feel	67:84	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	1	2	theme	preferred	167:175	arg1	sensation					185:193	a preferred tactile sensation	165:193	a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics	165:253	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	0	3	theme	Powders	58:64	arg1	"					44:44	"	44:44	" of Cosmetic Powders	44:64	Recognition Mechanism of the "Sara-sara Feel" of Cosmetic Powders.					
33456008	5	4	dep	slippery	702:709	arg1	feel					711:714	feel	711:714	feel	711:714	The relationship between certain physical properties, e.g., particle size distribution, and the slippery feel was analyzed to demonstrate how the subjects felt the slippery feel.					
33456008	1	5	dep	Sara-sara	71:79	arg1	feel					81:84	feel	81:84	feel	81:84	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	1	6	theme	tactile	177:183	arg1	Sara-sara					71:79	The Sara-sara feel	67:84	The Sara-sara feel	67:84	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	1	6	theme	tactile	177:183	arg1	sensation					185:193	a preferred tactile sensation	165:193	a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics	165:253	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	7	7	theme	powder	977:982	arg1	composition					943:953	the composition	939:953	the composition of spherical silicone powder	939:982	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	2	8	theme	powders	401:407	arg1	powder					335:340	cellulose powder	325:340	cellulose powder	325:340	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	2	8	theme	powders	401:407	arg1	mixes					386:390	various mixes	378:390	various mixes of these powders	378:407	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	2	8	theme	powders	401:407	arg1	powder					366:371	hydrophobized sericite powder	343:371	hydrophobized sericite powder	343:371	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	2	8	theme	powders	401:407	arg1	powder					317:322	silicone powder	308:322	silicone powder	308:322	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	7	9	theme	spherical	958:966	arg1	powder					977:982	spherical silicone powder	958:982	spherical silicone powder	958:982	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	4	10	theme	multiple	500:507	arg1	analysis					520:527	A multiple regression analysis	498:527	A multiple regression analysis	498:527	A multiple regression analysis showed that the Sara-sara feel was strongly correlated with a slippery feel.					
33456008	5	11	theme	certain	631:637	arg1	properties					648:657	certain physical properties	631:657	certain physical properties	631:657	The relationship between certain physical properties, e.g., particle size distribution, and the slippery feel was analyzed to demonstrate how the subjects felt the slippery feel.					
33456008	6	12	dep	slippery	883:890	arg1	feel					892:895	feel	892:895	feel	892:895	It was observed that as the friction coefficient µ k was reduced, most subjects strongly felt the slippery feel.					
33456008	7	13	theme	silicone	968:975	arg1	powder					977:982	spherical silicone powder	958:982	spherical silicone powder	958:982	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	1	14	dep	means	93:97	arg1	Sara-sara					71:79	The Sara-sara feel	67:84	The Sara-sara feel	67:84	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	1	14	dep	means	93:97	arg1	sensation					185:193	a preferred tactile sensation	165:193	a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics	165:253	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	1	14	dep	means	93:97	arg1	state					102:106	a state	100:106	means "a state in which things are not damp or sticky and feel dry	93:158	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	7	15	theme	amorphous	1087:1095	arg1	particles					1097:1105	amorphous particles	1087:1105	amorphous particles	1087:1105	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	5	16	theme	physical	639:646	arg1	properties					648:657	certain physical properties	631:657	certain physical properties	631:657	The relationship between certain physical properties, e.g., particle size distribution, and the slippery feel was analyzed to demonstrate how the subjects felt the slippery feel.					
33456008	5	17	dep	slippery	770:777	arg1	feel					779:782	feel	779:782	feel	779:782	The relationship between certain physical properties, e.g., particle size distribution, and the slippery feel was analyzed to demonstrate how the subjects felt the slippery feel.					
33456008	0	18	theme	Recognition	0:10	arg1	Mechanism					12:20	Recognition Mechanism	0:20	Recognition Mechanism of the "Sara-sara	0:38	Recognition Mechanism of the "Sara-sara Feel" of Cosmetic Powders.					
33456008	1	19	theme	people	200:205	arg1	skin					219:222	people touch human skin	200:222	people touch human skin	200:222	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	2	20	theme	cellulose	325:333	arg1	powder					335:340	cellulose powder	325:340	cellulose powder	325:340	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	1	21	theme	touch	207:211	arg1	skin					219:222	people touch human skin	200:222	people touch human skin	200:222	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	3	22	theme	highest	432:438	arg1	score					455:459	Sara-sara feel score	440:459	the highest Sara-sara feel score	428:459	It was found that the highest Sara-sara feel score was achieved by the silicone powder.					
33456008	4	23	theme	regression	509:518	arg1	analysis					520:527	A multiple regression analysis	498:527	A multiple regression analysis	498:527	A multiple regression analysis showed that the Sara-sara feel was strongly correlated with a slippery feel.					
33456008	1	24	theme	human	213:217	arg1	skin					219:222	people touch human skin	200:222	people touch human skin	200:222	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	3	25	theme	Sara-sara	440:448	arg1	score					455:459	Sara-sara feel score	440:459	the highest Sara-sara feel score	428:459	It was found that the highest Sara-sara feel score was achieved by the silicone powder.					
33456008	3	26	dep	score	455:459	arg1	feel					450:453	feel	450:453	feel	450:453	It was found that the highest Sara-sara feel score was achieved by the silicone powder.					
33456008	2	27	theme	silicone	308:315	arg1	powder					317:322	silicone powder	308:322	silicone powder	308:322	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	0	28	theme	Sara-sara	30:38	arg1	Mechanism					12:20	Recognition Mechanism	0:20	Recognition Mechanism of the "Sara-sara	0:38	Recognition Mechanism of the "Sara-sara Feel" of Cosmetic Powders.					
33456008	6	29	theme	coefficient	822:832	arg1	k					836:836	the friction coefficient µ k	809:836	the friction coefficient µ k	809:836	It was observed that as the friction coefficient µ k was reduced, most subjects strongly felt the slippery feel.					
33456008	5	30	theme	particle	666:673	arg1	distribution					680:691	particle size distribution	666:691	particle size distribution	666:691	The relationship between certain physical properties, e.g., particle size distribution, and the slippery feel was analyzed to demonstrate how the subjects felt the slippery feel.					
33456008	6	31	theme	friction	813:820	arg1	k					836:836	the friction coefficient µ k	809:836	the friction coefficient µ k	809:836	It was observed that as the friction coefficient µ k was reduced, most subjects strongly felt the slippery feel.					
33456008	6	32	theme	most	851:854	arg1	subjects					856:863	most subjects	851:863	most subjects	851:863	It was observed that as the friction coefficient µ k was reduced, most subjects strongly felt the slippery feel.					
33456008	7	33	theme	spherical	1028:1036	arg1	particles					1038:1046	spherical particles	1028:1046	spherical particles	1028:1046	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	2	34	theme	sericite	357:364	arg1	powder					366:371	hydrophobized sericite powder	343:371	hydrophobized sericite powder	343:371	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	2	35	theme	hydrophobized	343:355	arg1	powder					366:371	hydrophobized sericite powder	343:371	hydrophobized sericite powder	343:371	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	1	36	from	sticky	140:145	arg1	state					102:106	a state	100:106	means "a state in which things are not damp or sticky and feel dry	93:158	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	7	37	theme	contact	1007:1013	arg1	area					1015:1018	the contact area	1003:1018	the contact area between spherical particles	1003:1046	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	7	37	theme	contact	1007:1013	arg1	smaller					1051:1057	smaller	1051:1057	smaller	1051:1057	This coefficient slightly decreased when the composition of spherical silicone powder increased, because the contact area between spherical particles is smaller than that between plate and amorphous particles.					
33456008	2	38	dep	Sara-sara	275:283	arg1	feel					285:288	feel	285:288	feel	285:288	In this study, the Sara-sara feel was evaluated for silicone powder, cellulose powder, hydrophobized sericite powder, and various mixes of these powders.					
33456008	4	39	dep	slippery	591:598	arg1	feel					600:603	feel	600:603	feel	600:603	A multiple regression analysis showed that the Sara-sara feel was strongly correlated with a slippery feel.					
33456008	4	40	dep	Sara-sara	545:553	arg1	feel					555:558	feel	555:558	feel	555:558	A multiple regression analysis showed that the Sara-sara feel was strongly correlated with a slippery feel.					
33456008	1	41	from	damp	132:135	arg1	state					102:106	a state	100:106	means "a state in which things are not damp or sticky and feel dry	93:158	The Sara-sara feel, which means "a state in which things are not damp or sticky and feel dry," is a preferred tactile sensation when people touch human skin, hair, clothing, and cosmetics.					
33456008	0	42	theme	Cosmetic	49:56	arg1	Powders					58:64	Cosmetic Powders	49:64	Cosmetic Powders	49:64	Recognition Mechanism of the "Sara-sara Feel" of Cosmetic Powders.					
33456008	3	43	theme	silicone	481:488	arg1	powder					490:495	the silicone powder	477:495	the silicone powder	477:495	It was found that the highest Sara-sara feel score was achieved by the silicone powder.					
33456008	5	44	theme	size	675:678	arg1	distribution					680:691	particle size distribution	666:691	particle size distribution	666:691	The relationship between certain physical properties, e.g., particle size distribution, and the slippery feel was analyzed to demonstrate how the subjects felt the slippery feel.					
32445822	0	0	theme	drug	84:87	arg1	residue					89:95	diclofenac sodium drug residue	66:95	diclofenac sodium drug residue	66:95	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	6	1	theme	parameter	675:683	arg1	values					689:694	Thermodynamic parameter ΔG° values	661:694	Thermodynamic parameter ΔG° values	661:694	Thermodynamic parameter ΔG° values were -12.19, -13.74 and -15.67 kJ/mol at 20, 25 and 30 °C temperatures.					
32445822	1	2	theme	90	151:152	arg1	%					153:153	%	153:153	%	153:153	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	10	3	theme	sorption	1047:1054	arg1	method					1056:1061	The sorption method	1043:1061	The sorption method	1043:1061	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
32445822	0	4	theme	sodium	77:82	arg1	residue					89:95	diclofenac sodium drug residue	66:95	diclofenac sodium drug residue	66:95	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	1	5	theme	%	153:153	arg1	yield					155:159	90% yield	151:159	90% yield	151:159	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	3	6	with	water	515:519	arg1	elimination					538:548	maximum 85% elimination	526:548	maximum 85% elimination	526:548	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	3	7	from	elimination	479:489	arg1	water					515:519	water	515:519	water with maximum 85% elimination	515:548	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	0	8	theme	residue	89:95	arg1	removal					55:61	removal	55:61	removal of diclofenac sodium drug residue in water	55:104	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	0	9	from	removal	55:61	arg1	water					100:104	water	100:104	water	100:104	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	6	10	theme	Thermodynamic	661:673	arg1	values					689:694	Thermodynamic parameter ΔG° values	661:694	Thermodynamic parameter ΔG° values	661:694	Thermodynamic parameter ΔG° values were -12.19, -13.74 and -15.67 kJ/mol at 20, 25 and 30 °C temperatures.					
32445822	10	11	theme	water	1126:1130	arg1	body					1132:1135	any water body	1122:1135	any water body	1122:1135	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
32445822	2	12	theme	diclofenac	297:306	arg1	sodium					308:313	diclofenac sodium	297:313	diclofenac sodium	297:313	These were utilized to remove diclofenac sodium in water.					
32445822	2	13	used	utilized	278:285	arg2	These					267:271	These	267:271	These	267:271	These were utilized to remove diclofenac sodium in water.					
32445822	7	14	theme	ΔH°	782:784	arg1	values					772:777	The values	768:777	The values of ΔH° and ΔS°	768:792	The values of ΔH° and ΔS° were 8.58 and 20.84 kJ/mol.					
32445822	7	14	theme	ΔH°	782:784	arg1	8.58					799:802	8.58	799:802	8.58	799:802	The values of ΔH° and ΔS° were 8.58 and 20.84 kJ/mol.					
32445822	3	15	theme	sodium	505:510	arg1	elimination					479:489	the elimination	475:489	the elimination of diclofenac sodium in water with maximum 85% elimination	475:548	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	9	16	theme	water	1026:1030	arg1	resources					1032:1040	water resources	1026:1040	water resources	1026:1040	This adsorption method is fast, effective eco-friendly and low-cost as it may be used in natural circumstances of water resources.					
32445822	6	17	from	temperatures	754:765	arg1	-15.67 kJ/mol					720:732	-15.67 kJ/mol	720:732	-15.67 kJ/mol	720:732	Thermodynamic parameter ΔG° values were -12.19, -13.74 and -15.67 kJ/mol at 20, 25 and 30 °C temperatures.					
32445822	6	17	from	temperatures	754:765	arg1	-13.74					709:714	-13.74	709:714	-13.74	709:714	Thermodynamic parameter ΔG° values were -12.19, -13.74 and -15.67 kJ/mol at 20, 25 and 30 °C temperatures.					
32445822	6	17	from	temperatures	754:765	arg1	-12.19					701:706	-12.19	701:706	-12.19	701:706	Thermodynamic parameter ΔG° values were -12.19, -13.74 and -15.67 kJ/mol at 20, 25 and 30 °C temperatures.					
32445822	9	18	theme	adsorption	917:926	arg1	method					928:933	This adsorption method	912:933	This adsorption method	912:933	This adsorption method is fast, effective eco-friendly and low-cost as it may be used in natural circumstances of water resources.					
32445822	10	19	from	elimination	1086:1096	arg1	body					1132:1135	any water body	1122:1135	any water body	1122:1135	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
32445822	9	20	theme	resources	1032:1040	arg1	circumstances					1009:1021	natural circumstances	1001:1021	natural circumstances of water resources	1001:1040	This adsorption method is fast, effective eco-friendly and low-cost as it may be used in natural circumstances of water resources.					
32445822	1	21	theme	macromolecule	168:180	arg1	chitosan					182:189	macromolecule chitosan	168:189	macromolecule chitosan	168:189	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	0	22	theme	composite	22:30	arg1	iron					32:35	chitosan composite iron	13:35	chitosan composite iron	13:35	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	1	23	theme	TEM	255:257	arg1	EDX					261:263	TEM & EDX	255:263	TEM & EDX	255:263	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	1	23	theme	TEM	255:257	arg1	FT-IR					238:242	FT-IR	238:242	FT-IR	238:242	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	5	24	theme	Dubinin-Radushkevich	632:651	arg1	models					653:658	Dubinin-Radushkevich models	632:658	Dubinin-Radushkevich models	632:658	Data followed Langmuir, Temkin and Dubinin-Radushkevich models.					
32445822	0	25	attach	removal	55:61	arg2	residue					89:95	diclofenac sodium drug residue	66:95	diclofenac sodium drug residue	66:95	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	0	25	attach	removal	55:61	arg3	water					100:104	water	100:104	water	100:104	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	1	26	dep	prepared	141:148	arg1	yield					155:159	90% yield	151:159	90% yield	151:159	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	8	27	theme	Pseudo-first-order	822:839	arg1	mechanisms					867:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms were proposed for the adsorption.					
32445822	0	28	theme	chitosan	13:20	arg1	iron					32:35	chitosan composite iron	13:35	chitosan composite iron	13:35	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	1	29	theme	&	259:259	arg1	EDX					261:263	TEM & EDX	255:263	TEM & EDX	255:263	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	1	29	theme	&	259:259	arg1	FT-IR					238:242	FT-IR	238:242	FT-IR	238:242	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	3	30	theme	diclofenac	494:503	arg1	sodium					505:510	diclofenac sodium	494:510	diclofenac sodium	494:510	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	8	31	theme	film	852:855	arg1	mechanisms					867:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms were proposed for the adsorption.					
32445822	9	32	used	used	993:996	arg2	it					983:984	it	983:984	it	983:984	This adsorption method is fast, effective eco-friendly and low-cost as it may be used in natural circumstances of water resources.					
32445822	3	33	theme	maximum	526:532	arg1	elimination					538:548	maximum 85% elimination	526:548	maximum 85% elimination	526:548	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	8	34	theme	liquid	845:850	arg1	mechanisms					867:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms were proposed for the adsorption.					
32445822	0	35	theme	iron	32:35	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of chitosan composite iron	0:35	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	3	36	theme	85	534:535	arg1	%					536:536	%	536:536	%	536:536	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	7	37	theme	ΔS°	790:792	arg1	values					772:777	The values	768:777	The values of ΔH° and ΔS°	768:792	The values of ΔH° and ΔS° were 8.58 and 20.84 kJ/mol.					
32445822	7	37	theme	ΔS°	790:792	arg1	8.58					799:802	8.58	799:802	8.58	799:802	The values of ΔH° and ΔS° were 8.58 and 20.84 kJ/mol.					
32445822	1	38	dep	techniques	226:235	arg1	XRD					245:247	XRD	245:247	XRD	245:247	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	1	38	dep	techniques	226:235	arg1	EDX					261:263	TEM & EDX	255:263	TEM & EDX	255:263	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	1	38	dep	techniques	226:235	arg1	FT-IR					238:242	FT-IR	238:242	FT-IR	238:242	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	1	38	dep	techniques	226:235	arg1	SEM					250:252	SEM	250:252	SEM	250:252	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	8	39	theme	diffusion	857:865	arg1	mechanisms					867:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms	822:876	Pseudo-first-order and liquid film diffusion mechanisms were proposed for the adsorption.					
32445822	1	40	theme	Iron	107:110	arg1	nanoparticles					122:134	Iron composite nanoparticles	107:134	Iron composite nanoparticles	107:134	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	3	41	theme	adsorbent	438:446	arg1	amount					448:453	adsorbent amount	438:453	adsorbent amount	438:453	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	1	42	theme	composite	112:120	arg1	nanoparticles					122:134	Iron composite nanoparticles	107:134	Iron composite nanoparticles	107:134	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	4	43	with	spontaneous	568:578	arg1	exothermic					585:594	exothermic	585:594	exothermic	585:594	The sorption was spontaneous with exothermic.					
32445822	3	44	theme	contact	420:426	arg1	time					428:431	contact time	420:431	contact time	420:431	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	10	45	theme	financial	1151:1159	arg1	scale					1161:1165	a huge and financial scale	1140:1165	a huge and financial scale	1140:1165	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
32445822	1	46	theme	spectroscopic	212:224	arg1	techniques					226:235	spectroscopic techniques	212:235	spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX)	212:264	Iron composite nanoparticles were prepared (90% yield) using macromolecule chitosan and characterized by spectroscopic techniques (FT-IR, XRD, SEM, TEM & EDX).					
32445822	0	47	theme	diclofenac	66:75	arg1	residue					89:95	diclofenac sodium drug residue	66:95	diclofenac sodium drug residue	66:95	Synthesis of chitosan composite iron nanoparticles for removal of diclofenac sodium drug residue in water.					
32445822	3	48	theme	%	536:536	arg1	elimination					538:548	maximum 85% elimination	526:548	maximum 85% elimination	526:548	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	10	49	theme	huge	1142:1145	arg1	scale					1161:1165	a huge and financial scale	1140:1165	a huge and financial scale	1140:1165	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
32445822	10	50	theme	sodium	1112:1117	arg1	elimination					1086:1096	the elimination	1082:1096	the elimination of diclofenac sodium in any water body	1082:1135	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
32445822	3	51	theme	adjusted	329:336	arg1	parameters					338:347	The adjusted parameters	325:347	The adjusted parameters	325:347	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	3	51	theme	adjusted	329:336	arg1	400 μg/ L					354:362	400 μg/ L	354:362	400 μg/ L	354:362	The adjusted parameters were 400 μg/ L, 50.0 min., 5.0, 2.0 g/ L and 25.0 °C as concentration, contact time, pH, adsorbent amount and temperature for the elimination of diclofenac sodium in water with maximum 85% elimination.					
32445822	6	52	theme	ΔG°	685:687	arg1	values					689:694	Thermodynamic parameter ΔG° values	661:694	Thermodynamic parameter ΔG° values	661:694	Thermodynamic parameter ΔG° values were -12.19, -13.74 and -15.67 kJ/mol at 20, 25 and 30 °C temperatures.					
32445822	9	53	theme	natural	1001:1007	arg1	circumstances					1009:1021	natural circumstances	1001:1021	natural circumstances of water resources	1001:1040	This adsorption method is fast, effective eco-friendly and low-cost as it may be used in natural circumstances of water resources.					
32445822	10	54	theme	diclofenac	1101:1110	arg1	sodium					1112:1117	diclofenac sodium	1101:1117	diclofenac sodium	1101:1117	The sorption method may be applied for the elimination of diclofenac sodium in any water body at a huge and financial scale.					
34578853	7	0	theme	prebiotic	1129:1137	arg1	mixture					1139:1145	the prebiotic mixture	1125:1145	the prebiotic mixture	1125:1145	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	6	1	theme	study	891:895	arg1	end					880:882	the end	876:882	the end of the study	876:895	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	8	2	theme	scGOS/lcFOS	1383:1393	arg1	combination					1368:1378	the combination	1364:1378	the combination of scGOS/lcFOS and Lactofidus™	1364:1409	The use of the combination of scGOS/lcFOS and Lactofidus™ showed the effects observed for the oligosaccharides separately, as well as showing a synergistic impact on animal growth.					
34578853	1	3	theme	infant	122:127	arg1	formula					129:135	infant formula	122:135	infant formula providing nutritional solutions for infants not able to receive enough mother's milk	122:220	Human milk serves as a model for infant formula providing nutritional solutions for infants not able to receive enough mother's milk.					
34578853	9	4	theme	immune	1614:1619	arg1	features					1635:1642	immune and microbial features	1614:1642	immune and microbial features	1614:1642	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	5	5	theme	fecal	840:844	arg1	consistency					846:856	fecal consistency	840:856	fecal consistency	840:856	Body weight and fecal consistency were analyzed.					
34578853	3	6	from	supplementation	481:495	arg1	model					677:681	a preclinical model	663:681	a preclinical model of healthy suckling rats	663:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	8	7	theme	Lactofidus™	1399:1409	arg1	combination					1368:1378	the combination	1364:1378	the combination of scGOS/lcFOS and Lactofidus™	1364:1409	The use of the combination of scGOS/lcFOS and Lactofidus™ showed the effects observed for the oligosaccharides separately, as well as showing a synergistic impact on animal growth.					
34578853	9	8	theme	early	1647:1651	arg1	life					1653:1656	early life	1647:1656	early life	1647:1656	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	0	9	from	Effects	0:6	arg1	Rats					58:61	Suckling Rats	49:61	Suckling Rats	49:61	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	8	10	from	impact	1509:1514	arg1	growth					1526:1531	animal growth	1519:1531	animal growth	1519:1531	The use of the combination of scGOS/lcFOS and Lactofidus™ showed the effects observed for the oligosaccharides separately, as well as showing a synergistic impact on animal growth.					
34578853	3	11	theme	supplementation	481:495	arg1	effects					466:472	the effects	462:472	the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats	462:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	12	theme	healthy	686:692	arg1	rats					703:706	healthy suckling rats	686:706	healthy suckling rats	686:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	13	from	effects	466:472	arg1	model					677:681	a preclinical model	663:681	a preclinical model of healthy suckling rats	663:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	6	14	theme	chain	988:992	arg1	SCFA					1006:1009	SCFA	1006:1009	SCFA	1006:1009	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	6	14	theme	chain	988:992	arg1	acid					1000:1003	short chain fatty acid	982:1003	short chain fatty acid (SCFA) proportion	982:1021	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	6	15	theme	acid	1000:1003	arg1	proportion					1012:1021	short chain fatty acid (SCFA) proportion	982:1021	short chain fatty acid (SCFA) proportion	982:1021	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	3	16	with	combination	538:548	arg1	scGOS					607:611	scGOS	607:611	scGOS	607:611	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	16	with	combination	538:548	arg1	galactooligosaccharides					582:604	the prebiotics short-chain galactooligosaccharides	555:604	the prebiotics short-chain galactooligosaccharides (scGOS)	555:612	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	16	with	combination	538:548	arg1	fructooligosaccharides					629:650	long-chain fructooligosaccharides	618:650	long-chain fructooligosaccharides (lcFOS)	618:658	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	16	with	combination	538:548	arg1	lcFOS					653:657	lcFOS	653:657	lcFOS	653:657	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	17	theme	preclinical	665:675	arg1	model					677:681	a preclinical model	663:681	a preclinical model of healthy suckling rats	663:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	9	18	theme	products	1565:1572	arg1	use					1553:1555	the combined use	1540:1555	the combined use of both products	1540:1572	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	9	18	theme	products	1565:1572	arg1	strategy					1593:1600	a good strategy	1586:1600	a good strategy to modulate immune and microbial features in early life	1586:1656	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	3	19	with	postbiotic	504:513	arg1	scGOS					607:611	scGOS	607:611	scGOS	607:611	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	19	with	postbiotic	504:513	arg1	galactooligosaccharides					582:604	the prebiotics short-chain galactooligosaccharides	555:604	the prebiotics short-chain galactooligosaccharides (scGOS)	555:612	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	19	with	postbiotic	504:513	arg1	fructooligosaccharides					629:650	long-chain fructooligosaccharides	618:650	long-chain fructooligosaccharides (lcFOS)	618:658	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	19	with	postbiotic	504:513	arg1	lcFOS					653:657	lcFOS	653:657	lcFOS	653:657	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	1	20	theme	nutritional	147:157	arg1	solutions					159:167	nutritional solutions	147:167	nutritional solutions for infants not able to receive enough mother's milk	147:220	Human milk serves as a model for infant formula providing nutritional solutions for infants not able to receive enough mother's milk.					
34578853	7	21	theme	softer	1224:1229	arg1	feces					1231:1235	softer feces	1224:1235	softer feces	1224:1235	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	2	22	theme	latest	349:354	arg1	milk					362:365	the latest human milk insights	345:374	the latest human milk insights	345:374	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	3	23	from	model	677:681	arg1	effects					466:472	the effects	462:472	the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats	462:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	6	24	theme	immunoglobulin	898:911	arg1	profile					918:924	immunoglobulin (Ig) profile	898:924	immunoglobulin (Ig) profile	898:924	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	4	25	theme	PRE	797:799	arg1	groups					801:806	PRE groups	797:806	PRE groups	797:806	Pups were supplemented daily with LactofidusTM (POST group) and/or scGOS/lcFOS (P+P and PRE groups, respectively).					
34578853	6	26	theme	short	982:986	arg1	SCFA					1006:1009	SCFA	1006:1009	SCFA	1006:1009	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	6	26	theme	short	982:986	arg1	acid					1000:1003	short chain fatty acid	982:1003	short chain fatty acid (SCFA) proportion	982:1021	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	0	27	theme	Postbiotic	13:22	arg1	Mixture					38:44	a Postbiotic and Prebiotic Mixture	11:44	a Postbiotic and Prebiotic Mixture	11:44	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	6	28	theme	fatty	994:998	arg1	SCFA					1006:1009	SCFA	1006:1009	SCFA	1006:1009	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	6	28	theme	fatty	994:998	arg1	acid					1000:1003	short chain fatty acid	982:1003	short chain fatty acid (SCFA) proportion	982:1021	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	9	29	theme	microbial	1625:1633	arg1	features					1635:1642	immune and microbial features	1614:1642	immune and microbial features	1614:1642	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	7	30	theme	differential	1174:1185	arg1	effects					1187:1193	differential effects	1174:1193	differential effects	1174:1193	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	3	31	theme	suckling	694:701	arg1	rats					703:706	healthy suckling rats	686:706	healthy suckling rats	686:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	7	32	with	supplementation	1044:1058	arg1	interventions					1081:1093	all nutritional interventions	1065:1093	all nutritional interventions	1065:1093	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	1	33	theme	Human	89:93	arg1	milk					95:98	Human milk	89:98	Human milk	89:98	Human milk serves as a model for infant formula providing nutritional solutions for infants not able to receive enough mother's milk.					
34578853	1	33	theme	Human	89:93	arg1	model					112:116	a model	110:116	a model for infant formula providing nutritional solutions for infants not able to receive enough mother's milk	110:220	Human milk serves as a model for infant formula providing nutritional solutions for infants not able to receive enough mother's milk.					
34578853	8	34	theme	animal	1519:1524	arg1	growth					1526:1531	animal growth	1519:1531	animal growth	1519:1531	The use of the combination of scGOS/lcFOS and Lactofidus™ showed the effects observed for the oligosaccharides separately, as well as showing a synergistic impact on animal growth.					
34578853	4	35	dep	scGOS/lcFOS	776:786	arg1	P+P					789:791	P+P	789:791	P+P	789:791	Pups were supplemented daily with LactofidusTM (POST group) and/or scGOS/lcFOS (P+P and PRE groups, respectively).					
34578853	4	35	dep	scGOS/lcFOS	776:786	arg1	groups					801:806	PRE groups	797:806	PRE groups	797:806	Pups were supplemented daily with LactofidusTM (POST group) and/or scGOS/lcFOS (P+P and PRE groups, respectively).					
34578853	0	36	theme	Prebiotic	28:36	arg1	Mixture					38:44	a Postbiotic and Prebiotic Mixture	11:44	a Postbiotic and Prebiotic Mixture	11:44	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	3	37	theme	rats	703:706	arg1	model					677:681	a preclinical model	663:681	a preclinical model of healthy suckling rats	663:706	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	6	38	theme	microbiota	955:964	arg1	composition					966:976	microbiota composition	955:976	microbiota composition	955:976	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	7	39	theme	gene	1336:1339	arg1	expression					1341:1350	Toll-like receptors gene expression	1316:1350	Toll-like receptors gene expression	1316:1350	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	1	40	theme	able	185:188	arg1	infants					173:179	infants	173:179	infants not able to receive enough mother's milk	173:220	Human milk serves as a model for infant formula providing nutritional solutions for infants not able to receive enough mother's milk.					
34578853	7	41	theme	nutritional	1069:1079	arg1	interventions					1081:1093	all nutritional interventions	1065:1093	all nutritional interventions	1065:1093	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	3	42	theme	short-chain	570:580	arg1	scGOS					607:611	scGOS	607:611	scGOS	607:611	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	42	theme	short-chain	570:580	arg1	galactooligosaccharides					582:604	the prebiotics short-chain galactooligosaccharides	555:604	the prebiotics short-chain galactooligosaccharides (scGOS)	555:612	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	9	43	theme	combined	1544:1551	arg1	use					1553:1555	the combined use	1540:1555	the combined use of both products	1540:1572	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	9	43	theme	combined	1544:1551	arg1	strategy					1593:1600	a good strategy	1586:1600	a good strategy to modulate immune and microbial features in early life	1586:1656	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	3	44	with	supplementation	481:495	arg1	postbiotic					504:513	a postbiotic	502:513	a postbiotic (LactofidusTM)	502:528	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	44	with	supplementation	481:495	arg1	combination					538:548	its combination	534:548	its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS)	534:658	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	44	with	supplementation	481:495	arg1	LactofidusTM					516:527	LactofidusTM	516:527	LactofidusTM	516:527	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	8	45	theme	synergistic	1497:1507	arg1	impact					1509:1514	a synergistic impact	1495:1514	a synergistic impact on animal growth	1495:1531	The use of the combination of scGOS/lcFOS and Lactofidus™ showed the effects observed for the oligosaccharides separately, as well as showing a synergistic impact on animal growth.					
34578853	7	46	theme	Toll-like	1316:1324	arg1	receptors					1326:1334	Toll-like receptors	1316:1334	Toll-like receptors gene expression	1316:1350	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	3	47	theme	study	441:445	arg1	aim					429:431	The aim	425:431	The aim of this study	425:445	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	0	48	theme	Mixture	38:44	arg1	Immunity					79:86	Immunity	79:86	Immunity	79:86	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	0	48	theme	Mixture	38:44	arg1	Effects					0:6	Effects	0:6	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity	0:86	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	0	48	theme	Mixture	38:44	arg1	Microbiota					64:73	Microbiota	64:73	Microbiota	64:73	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	7	49	theme	receptors	1326:1334	arg1	expression					1341:1350	Toll-like receptors gene expression	1316:1350	Toll-like receptors gene expression	1316:1350	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	7	50	theme	SCFA	1278:1281	arg1	profile					1283:1289	SCFA profile	1278:1289	SCFA profile	1278:1289	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	2	51	dep	composition	256:266	arg1	the					252:254	the	252:254	the	252:254	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	4	52	theme	POST	757:760	arg1	LactofidusTM					743:754	LactofidusTM	743:754	LactofidusTM (POST group)	743:767	Pups were supplemented daily with LactofidusTM (POST group) and/or scGOS/lcFOS (P+P and PRE groups, respectively).					
34578853	4	52	theme	POST	757:760	arg1	group					762:766	POST group	757:766	POST group	757:766	Pups were supplemented daily with LactofidusTM (POST group) and/or scGOS/lcFOS (P+P and PRE groups, respectively).					
34578853	8	53	theme	combination	1368:1378	arg1	use					1357:1359	The use	1353:1359	The use of the combination of scGOS/lcFOS and Lactofidus™	1353:1409	The use of the combination of scGOS/lcFOS and Lactofidus™ showed the effects observed for the oligosaccharides separately, as well as showing a synergistic impact on animal growth.					
34578853	3	54	theme	prebiotics	559:568	arg1	scGOS					607:611	scGOS	607:611	scGOS	607:611	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	54	theme	prebiotics	559:568	arg1	galactooligosaccharides					582:604	the prebiotics short-chain galactooligosaccharides	555:604	the prebiotics short-chain galactooligosaccharides (scGOS)	555:612	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	2	55	theme	milk	295:298	arg1	functionality					272:284	functionality	272:284	functionality	272:284	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	2	55	theme	milk	295:298	arg1	composition					256:266	composition	256:266	composition	256:266	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	2	56	theme	Infant	223:228	arg1	formulas					230:237	Infant formulas	223:237	Infant formulas	223:237	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	2	57	theme	human	289:293	arg1	milk					295:298	human milk	289:298	human milk	289:298	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	7	58	theme	microbiota	1251:1260	arg1	composition					1262:1272	microbiota composition	1251:1272	microbiota composition	1251:1272	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
34578853	6	59	theme	gene	938:941	arg1	expression					943:952	intestinal gene expression	927:952	intestinal gene expression	927:952	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	9	60	theme	good	1588:1591	arg1	use					1553:1555	the combined use	1540:1555	the combined use of both products	1540:1572	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	9	60	theme	good	1588:1591	arg1	strategy					1593:1600	a good strategy	1586:1600	a good strategy to modulate immune and microbial features in early life	1586:1656	Thus, the combined use of both products seems to be a good strategy to modulate immune and microbial features in early life.					
34578853	3	61	theme	long-chain	618:627	arg1	lcFOS					653:657	lcFOS	653:657	lcFOS	653:657	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	3	61	theme	long-chain	618:627	arg1	fructooligosaccharides					629:650	long-chain fructooligosaccharides	618:650	long-chain fructooligosaccharides (lcFOS)	618:658	The aim of this study was to examine the effects of the supplementation with a postbiotic (LactofidusTM) and its combination with the prebiotics short-chain galactooligosaccharides (scGOS) and long-chain fructooligosaccharides (lcFOS) in a preclinical model of healthy suckling rats.					
34578853	2	62	dep	milk	362:365	arg1	insights					367:374	insights	367:374	the latest human milk insights	345:374	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	5	63	theme	Body	824:827	arg1	weight					829:834	Body weight	824:834	Body weight	824:834	Body weight and fecal consistency were analyzed.					
34578853	2	64	theme	human	356:360	arg1	milk					362:365	the latest human milk insights	345:374	the latest human milk insights	345:374	Infant formulas aim to mimic the composition and functionality of human milk by providing ingredients reflecting those of the latest human milk insights, such as prebiotics, probiotics and postbiotics.					
34578853	6	65	theme	intestinal	927:936	arg1	expression					943:952	intestinal gene expression	927:952	intestinal gene expression	927:952	At the end of the study, immunoglobulin (Ig) profile, intestinal gene expression, microbiota composition and short chain fatty acid (SCFA) proportion were quantified.					
34578853	0	66	theme	Suckling	49:56	arg1	Rats					58:61	Suckling Rats	49:61	Suckling Rats	49:61	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	0	67	dep	Effects	0:6	arg1	Immunity					79:86	Immunity	79:86	Immunity	79:86	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	0	67	dep	Effects	0:6	arg1	Effects					0:6	Effects	0:6	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity	0:86	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	0	67	dep	Effects	0:6	arg1	Microbiota					64:73	Microbiota	64:73	Microbiota	64:73	Effects of a Postbiotic and Prebiotic Mixture on Suckling Rats' Microbiota and Immunity.					
34578853	7	68	theme	Ig	1109:1110	arg1	profile					1112:1118	the Ig profile	1105:1118	the Ig profile	1105:1118	The supplementation with all nutritional interventions modulated the Ig profile, but the prebiotic mixture and the postbiotic induced differential effects: whereas scGOS/lcFOS induced softer feces and modulated microbiota composition and SCFA profile, Lactofidus™ upregulated Toll-like receptors gene expression.					
32088223	9	0	theme	high	1206:1209	arg1	capacity					1222:1229	its high adsorption capacity	1202:1229	its high adsorption capacity	1202:1229	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	5	1	theme	200.0	790:794	arg1	capacity					778:785	a maximum adsorption capacity	757:785	a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	757:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	9	2	theme	CNF-Fe	1300:1305	arg1	FeS					1310:1312	CNF-Fe(0)@FeS	1300:1312	CNF-Fe(0)@FeS	1300:1312	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	5	3	theme	blue	707:710	arg1	dye					717:719	cationic Methylene blue (MB) dye	688:719	cationic Methylene blue (MB) dye	688:719	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	7	4	from	370 nm	1030:1035	arg1	peak					1022:1025	peak	1022:1025	peak at 370 nm	1022:1035	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	7	4	from	370 nm	1030:1035	arg1	3 min					1015:1019	3 min	1015:1019	3 min	1015:1019	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	9	5	theme	promising	1316:1324	arg1	sorbent					1326:1332	a promising sorbent	1314:1332	a promising sorbent for the treatment industrial dye effluents	1314:1375	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	2	6	theme	CNF-Fe	326:331	arg1	FeS					336:338	CNF-Fe(0)@FeS	326:338	CNF-Fe(0)@FeS	326:338	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	9	7	theme	industrial	1352:1361	arg1	effluents					1367:1375	the treatment industrial dye effluents	1338:1375	the treatment industrial dye effluents	1338:1375	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	4	8	theme	nanocomposite	534:546	arg1	agglomeration					517:529	agglomeration	517:529	agglomeration of nanocomposite	517:546	Anchoring on cellulose nano fibers prevented agglomeration of nanocomposite and furthermore, coating of FeS2 helped in the preservation of Fe(0).					
32088223	5	9	theme	red	739:741	arg1	dye					748:750	anionic Congo red (CR) dye	725:750	anionic Congo red (CR) dye	725:750	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	9	theme	red	739:741	arg1	CR					744:745	CR	744:745	CR	744:745	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	9	10	theme	treatment	1342:1350	arg1	effluents					1367:1375	the treatment industrial dye effluents	1338:1375	the treatment industrial dye effluents	1338:1375	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	6	11	theme	regression	880:889	arg1	coefficients					891:902	regression coefficients >0.98	880:908	regression coefficients >0.98	880:908	Both MB and CR dye followed Pseudo second kinetic with regression coefficients >0.98.					
32088223	3	12	theme	high	432:435	arg1	TEM					448:450	high resolution TEM	432:450	high resolution TEM	432:450	Formation of FeS2 was confirmed by high resolution TEM and XPS techniques.					
32088223	0	13	theme	Potential	80:88	arg1	bionanocomposite					90:105	Potential bionanocomposite	80:105	Potential bionanocomposite	80:105	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	5	14	theme	111.1 mg/g	800:809	arg1	capacity					778:785	a maximum adsorption capacity	757:785	a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	757:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	15	theme	maximum	759:765	arg1	capacity					778:785	a maximum adsorption capacity	757:785	a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	757:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	1	16	theme	macromolecule	149:161	arg1	nanofiber					180:188	Biological macromolecule namely cellulose nanofiber	138:188	Biological macromolecule namely cellulose nanofiber	138:188	Biological macromolecule namely cellulose nanofiber was synthesized from agrowaste sugarcane bagasse.					
32088223	4	17	from	Anchoring	472:480	arg1	fibers					500:505	cellulose nano fibers	485:505	cellulose nano fibers	485:505	Anchoring on cellulose nano fibers prevented agglomeration of nanocomposite and furthermore, coating of FeS2 helped in the preservation of Fe(0).					
32088223	5	18	theme	anionic	725:731	arg1	dye					748:750	anionic Congo red (CR) dye	725:750	anionic Congo red (CR) dye	725:750	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	18	theme	anionic	725:731	arg1	CR					744:745	CR	744:745	CR	744:745	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	4	19	theme	Fe	611:612	arg1	preservation					595:606	the preservation	591:606	the preservation of Fe(0)	591:615	Anchoring on cellulose nano fibers prevented agglomeration of nanocomposite and furthermore, coating of FeS2 helped in the preservation of Fe(0).					
32088223	0	20	theme	nano	13:16	arg1	FeS					24:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	7	21	from	reduction	1057:1065	arg1	intensity					1070:1078	intensity	1070:1078	intensity	1070:1078	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	7	22	theme	adsorption/degradation	967:988	arg1	kinetics					955:962	kinetics	955:962	kinetics of adsorption/degradation	955:988	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	7	23	theme	%	1055:1055	arg1	reduction					1057:1065	70% reduction	1053:1065	70% reduction in intensity	1053:1078	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	1	24	theme	cellulose	170:178	arg1	nanofiber					180:188	Biological macromolecule namely cellulose nanofiber	138:188	Biological macromolecule namely cellulose nanofiber	138:188	Biological macromolecule namely cellulose nanofiber was synthesized from agrowaste sugarcane bagasse.					
32088223	0	25	theme	Hierarchical	0:11	arg1	FeS					24:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	9	26	theme	adsorption	1211:1220	arg1	capacity					1222:1229	its high adsorption capacity	1202:1229	its high adsorption capacity	1202:1229	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	2	27	theme	@	335:335	arg1	FeS					336:338	CNF-Fe(0)@FeS	326:338	CNF-Fe(0)@FeS	326:338	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	5	28	theme	Congo	733:737	arg1	dye					748:750	anionic Congo red (CR) dye	725:750	anionic Congo red (CR) dye	725:750	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	28	theme	Congo	733:737	arg1	CR					744:745	CR	744:745	CR	744:745	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	7	29	located	observed	1084:1091	arg2	reduction					1057:1065	70% reduction	1053:1065	70% reduction in intensity	1053:1078	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	7	29	located	observed	1084:1091	arg1	660 nm					1096:1101	660 nm	1096:1101	660 nm	1096:1101	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	5	30	theme	nano	636:639	arg1	composite					641:649	the prepared nano composite	623:649	the prepared nano composite	623:649	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	30	theme	nano	636:639	arg1	effective					660:668	effective	660:668	effective	660:668	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	2	31	theme	various	368:374	arg1	techniques					385:394	various spectral techniques	368:394	various spectral techniques	368:394	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	2	32	theme	spectral	376:383	arg1	techniques					385:394	various spectral techniques	368:394	various spectral techniques	368:394	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	5	33	dep	blue	707:710	arg1	MB					713:714	MB	713:714	MB	713:714	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	7	34	dep	fast	999:1002	arg1	disappeared					1037:1047	disappeared	1037:1047	disappeared	1037:1047	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	1	35	theme	Biological	138:147	arg1	nanofiber					180:188	Biological macromolecule namely cellulose nanofiber	138:188	Biological macromolecule namely cellulose nanofiber	138:188	Biological macromolecule namely cellulose nanofiber was synthesized from agrowaste sugarcane bagasse.					
32088223	5	36	theme	dye	717:719	arg1	removal					677:683	the removal	673:683	the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	673:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	9	37	theme	degradation	1232:1242	arg1	potential					1244:1252	degradation potential	1232:1252	degradation potential	1232:1252	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	5	38	from	removal	677:683	arg1	composite					641:649	the prepared nano composite	623:649	the prepared nano composite	623:649	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	38	from	removal	677:683	arg1	effective					660:668	effective	660:668	effective	660:668	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	39	theme	adsorption	767:776	arg1	capacity					778:785	a maximum adsorption capacity	757:785	a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	757:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	9	40	theme	@	1309:1309	arg1	FeS					1310:1312	CNF-Fe(0)@FeS	1300:1312	CNF-Fe(0)@FeS	1300:1312	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	5	41	from	effective	660:668	arg1	removal					677:683	the removal	673:683	the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	673:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	42	with	removal	677:683	arg1	capacity					778:785	a maximum adsorption capacity	757:785	a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	757:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	43	theme	dye	748:750	arg1	removal					677:683	the removal	673:683	the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively	673:822	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	1	44	theme	agrowaste	211:219	arg1	bagasse					231:237	agrowaste sugarcane bagasse	211:237	agrowaste sugarcane bagasse	211:237	Biological macromolecule namely cellulose nanofiber was synthesized from agrowaste sugarcane bagasse.					
32088223	8	45	theme	adsorbed	1166:1173	arg1	molecules					1179:1187	adsorbed dye molecules	1166:1187	adsorbed dye molecules	1166:1187	Further FTIR and XPS revealed evidence for the degradation of adsorbed dye molecules.					
32088223	0	46	theme	dyes	132:135	arg1	treatment					111:119	treatment	111:119	treatment of organic dyes	111:135	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	7	47	theme	MB	926:927	arg1	dye					929:931	MB dye	926:931	MB dye	926:931	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	3	48	theme	resolution	437:446	arg1	TEM					448:450	high resolution TEM	432:450	high resolution TEM	432:450	Formation of FeS2 was confirmed by high resolution TEM and XPS techniques.					
32088223	0	49	theme	@	23:23	arg1	FeS					24:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	4	50	theme	nano	495:498	arg1	fibers					500:505	cellulose nano fibers	485:505	cellulose nano fibers	485:505	Anchoring on cellulose nano fibers prevented agglomeration of nanocomposite and furthermore, coating of FeS2 helped in the preservation of Fe(0).					
32088223	0	51	theme	organic	124:130	arg1	dyes					132:135	organic dyes	124:135	organic dyes	124:135	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	3	52	theme	XPS	456:458	arg1	techniques					460:469	XPS techniques	456:469	XPS techniques	456:469	Formation of FeS2 was confirmed by high resolution TEM and XPS techniques.					
32088223	9	53	theme	dye	1363:1365	arg1	effluents					1367:1375	the treatment industrial dye effluents	1338:1375	the treatment industrial dye effluents	1338:1375	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	4	54	theme	cellulose	485:493	arg1	fibers					500:505	cellulose nano fibers	485:505	cellulose nano fibers	485:505	Anchoring on cellulose nano fibers prevented agglomeration of nanocomposite and furthermore, coating of FeS2 helped in the preservation of Fe(0).					
32088223	0	55	attach	derived	55:61	arg1	agrowaste					68:76	agrowaste	68:76	agrowaste	68:76	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	0	55	attach	derived	55:61	arg1	bionanocomposite					90:105	Potential bionanocomposite	80:105	Potential bionanocomposite	80:105	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	0	55	attach	derived	55:61	arg2	nanofibres					44:53	cellulose nanofibres	34:53	cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes	34:135	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	8	56	theme	molecules	1179:1187	arg1	degradation					1151:1161	the degradation	1147:1161	the degradation of adsorbed dye molecules	1147:1187	Further FTIR and XPS revealed evidence for the degradation of adsorbed dye molecules.					
32088223	9	57	theme	preparation	1266:1276	arg1	ease					1258:1261	ease	1258:1261	ease of preparation using agrowaste	1258:1292	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	9	57	theme	preparation	1266:1276	arg1	capacity					1222:1229	its high adsorption capacity	1202:1229	its high adsorption capacity	1202:1229	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	9	57	theme	preparation	1266:1276	arg1	potential					1244:1252	degradation potential	1232:1252	degradation potential	1232:1252	Considering its high adsorption capacity, degradation potential and ease of preparation using agrowaste makes CNF-Fe(0)@FeS a promising sorbent for the treatment industrial dye effluents.					
32088223	1	58	theme	sugarcane	221:229	arg1	bagasse					231:237	agrowaste sugarcane bagasse	211:237	agrowaste sugarcane bagasse	211:237	Biological macromolecule namely cellulose nanofiber was synthesized from agrowaste sugarcane bagasse.					
32088223	5	59	theme	prepared	627:634	arg1	composite					641:649	the prepared nano composite	623:649	the prepared nano composite	623:649	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	5	59	theme	prepared	627:634	arg1	effective					660:668	effective	660:668	effective	660:668	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	2	60	theme	nano	262:265	arg1	0					270:270	0	270:270	0	270:270	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	2	60	theme	nano	262:265	arg1	Fe					267:268	hierarchical nano Fe	249:268	hierarchical nano Fe(0)	249:271	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	2	61	theme	cellulose	295:303	arg1	nanofibers					305:314	cellulose nanofibers	295:314	cellulose nanofibers	295:314	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	8	62	theme	Further	1104:1110	arg1	FTIR					1112:1115	Further FTIR	1104:1115	Further FTIR	1104:1115	Further FTIR and XPS revealed evidence for the degradation of adsorbed dye molecules.					
32088223	0	63	theme	cellulose	34:42	arg1	nanofibres					44:53	cellulose nanofibres	34:53	cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes	34:135	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	3	64	theme	FeS2	410:413	arg1	Formation					397:405	Formation	397:405	Formation of FeS2	397:413	Formation of FeS2 was confirmed by high resolution TEM and XPS techniques.					
32088223	2	65	theme	hierarchical	249:260	arg1	0					270:270	0	270:270	0	270:270	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	2	65	theme	hierarchical	249:260	arg1	Fe					267:268	hierarchical nano Fe	249:268	hierarchical nano Fe(0)	249:271	Further, hierarchical nano Fe(0) - FeS was anchored on cellulose nanofibers to obtain CNF-Fe(0)@FeS and it was characterized by various spectral techniques.					
32088223	8	66	theme	dye	1175:1177	arg1	molecules					1179:1187	adsorbed dye molecules	1166:1187	adsorbed dye molecules	1166:1187	Further FTIR and XPS revealed evidence for the degradation of adsorbed dye molecules.					
32088223	5	67	theme	cationic	688:695	arg1	dye					717:719	cationic Methylene blue (MB) dye	688:719	cationic Methylene blue (MB) dye	688:719	Thus the prepared nano composite was very effective in the removal of cationic Methylene blue (MB) dye and anionic Congo red (CR) dye with a maximum adsorption capacity of 200.0 and 111.1 mg/g respectively.					
32088223	0	68	theme	Fe	18:19	arg1	FeS					24:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS	0:26	Hierarchical nano Fe(0)@FeS doped cellulose nanofibres derived from agrowaste - Potential bionanocomposite for treatment of organic dyes.					
32088223	7	69	theme	70	1053:1054	arg1	%					1055:1055	%	1055:1055	%	1055:1055	Especially for MB dye, it was observed that kinetics of adsorption/degradation was very fast and within 3 min, peak at 370 nm disappeared and 70% reduction in intensity was observed at 660 nm.					
32088223	4	70	theme	FeS2	576:579	arg1	coating					565:571	coating	565:571	coating of FeS2	565:579	Anchoring on cellulose nano fibers prevented agglomeration of nanocomposite and furthermore, coating of FeS2 helped in the preservation of Fe(0).					
33602987	7	0	theme	NK	1298:1299	arg1	cells					1301:1305	NK cells	1298:1305	NK cells	1298:1305	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	8	1	located	detected	1440:1447	arg2	glycans					1385:1391	anti-inflammatory glycans	1367:1391	anti-inflammatory glycans	1367:1391	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	8	1	located	detected	1440:1447	arg2	activation					1424:1433	antibody-dependent NK cell activation	1397:1433	antibody-dependent NK cell activation	1397:1433	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	8	1	located	detected	1440:1447	arg1	women					1461:1465	pregnant women	1452:1465	pregnant women with malaria	1452:1478	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	3	2	theme	pregnant	550:557	arg1	women					559:563	pregnant women	550:563	pregnant women with malaria	550:576	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	4	3	theme	antibodies	647:656	arg1	capacity					631:638	the capacity	627:638	the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3	627:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	1	4	theme	cells	198:202	arg1	activation					170:179	antibody-mediated activation	152:179	antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity,	152:295	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	4	theme	cells	198:202	arg1	secretion					305:313	the secretion	301:313	the secretion of cytokines and chemokines	301:341	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	4	5	theme	pregnant	663:670	arg1	women					672:676	pregnant women	663:676	pregnant women	663:676	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	5	6	theme	pregnant	878:885	arg1	women					887:891	pregnant women	878:891	pregnant women with malaria	878:904	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	5	7	located	observed	866:873	arg1	women					887:891	pregnant women	878:891	pregnant women with malaria	878:904	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	5	7	located	observed	866:873	arg2	activation					851:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	5	8	theme	NK	843:844	arg1	activation					851:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	8	9	theme	antibody	1509:1516	arg1	features					1518:1525	these antibody features	1503:1525	these antibody features	1503:1525	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	1	10	theme	effector	225:232	arg1	cytotoxicity					283:294	antibody-dependent cellular cytotoxicity	255:294	antibody-dependent cellular cytotoxicity	255:294	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	10	theme	effector	225:232	arg1	functions					234:242	Fc effector functions	222:242	Fc effector functions	222:242	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	11	theme	Plasmodium	122:131	arg1	falciparum					133:142	Plasmodium falciparum	122:142	Plasmodium falciparum	122:142	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	3	12	theme	antibody-mediated	469:485	arg1	NK					503:504	NK	503:504	NK	503:504	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	3	12	theme	antibody-mediated	469:485	arg1	killer					495:500	antibody-mediated natural killer	469:500	antibody-mediated natural killer (NK) cells activation	469:522	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	0	13	theme	malaria	56:62	arg1	women					81:85	malaria exposed pregnant women	56:85	malaria exposed pregnant women	56:85	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	6	14	theme	IgG	1037:1039	arg1	antibodies					1041:1050	IgG antibodies	1037:1050	IgG antibodies	1037:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	0	15	from	activation	18:27	arg1	women					81:85	malaria exposed pregnant women	56:85	malaria exposed pregnant women	56:85	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	4	16	theme	placental	684:692	arg1	malaria					694:700	placental malaria	684:700	placental malaria	684:700	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	8	17	with	women	1461:1465	arg1	malaria					1472:1478	malaria	1472:1478	malaria	1472:1478	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	0	18	theme	pregnant	72:79	arg1	women					81:85	malaria exposed pregnant women	56:85	malaria exposed pregnant women	56:85	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	1	19	theme	innate	184:189	arg1	cells					198:202	innate immune cells	184:202	innate immune cells	184:202	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	3	20	link	N-linked	530:537	arg1	glycans					539:545	Fc N-linked glycans	527:545	Fc N-linked glycans	527:545	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	5	21	with	women	887:891	arg1	malaria					898:904	malaria	898:904	malaria	898:904	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	8	22	theme	cell	1419:1422	arg1	activation					1424:1433	antibody-dependent NK cell activation	1397:1433	antibody-dependent NK cell activation	1397:1433	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	6	23	theme	anti-inflammatory	993:1009	arg1	patterns					1025:1032	Elevated anti-inflammatory glycosylation patterns	984:1032	Elevated anti-inflammatory glycosylation patterns of IgG antibodies	984:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	2	24	theme	N-linked	431:438	arg1	glycans					440:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	6	25	theme	malaria	1100:1106	arg1	infection					1108:1116	malaria infection	1100:1116	malaria infection	1100:1116	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	3	26	theme	Fc	527:528	arg1	glycans					539:545	Fc N-linked glycans	527:545	Fc N-linked glycans	527:545	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	2	27	theme	G	420:420	arg1	glycans					440:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	7	28	theme	Fc	1231:1232	arg1	glycans					1243:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	1	29	theme	Immune	88:93	arg1	responses					104:112	Immune effector responses	88:112	Immune effector responses against Plasmodium falciparum	88:142	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	4	30	theme	NK	738:739	arg1	activation					746:755	NK cell activation	738:755	NK cell activation	738:755	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	2	31	theme	glycans	440:446	arg1	composition					390:400	the composition	386:400	the composition of immunoglobulin G (IgG) Fc N-linked glycans	386:446	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	7	32	theme	pregnancy-associated	1192:1211	arg1	glycans					1243:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	2	33	theme	Fc	428:429	arg1	glycans					440:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	0	34	theme	natural	32:38	arg1	cells					47:51	natural killer cells	32:51	natural killer cells	32:51	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	0	35	theme	cells	47:51	arg1	activation					18:27	activation	18:27	activation of natural killer cells in malaria exposed pregnant women	18:85	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	6	36	theme	healthy	1142:1148	arg1	controls					1163:1170	healthy non-pregnant controls	1142:1170	healthy non-pregnant controls	1142:1170	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	4	37	theme	placental	772:780	arg1	antigens					801:808	placental malaria-associated antigens DBL2 and DBL3	772:822	placental malaria-associated antigens DBL2 and DBL3	772:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	37	theme	placental	772:780	arg1	DBL3					819:822	DBL3	819:822	DBL3	819:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	37	theme	placental	772:780	arg1	DBL2					810:813	DBL2	810:813	DBL2	810:813	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	6	38	gly	glycosylation	1011:1023	arg1	antibodies					1041:1050	IgG antibodies	1037:1050	IgG antibodies	1037:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	3	39	theme	killer	495:500	arg1	cells					507:511	antibody-mediated natural killer (NK) cells	469:511	antibody-mediated natural killer (NK) cells activation	469:522	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	2	40	link	N-linked	431:438	arg1	glycans					440:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	immunoglobulin G (IgG) Fc N-linked glycans	405:446	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	7	41	theme	pregnant	1310:1317	arg1	women					1319:1323	pregnant women	1310:1323	pregnant women with malaria infection	1310:1346	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	3	42	theme	cells	507:511	arg1	activation					513:522	antibody-mediated natural killer (NK) cells activation	469:522	antibody-mediated natural killer (NK) cells activation	469:522	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	1	43	theme	cellular	274:281	arg1	cytotoxicity					283:294	antibody-dependent cellular cytotoxicity	255:294	antibody-dependent cellular cytotoxicity	255:294	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	7	44	theme	cells	1301:1305	arg1	activation					1284:1293	the antibody-mediated activation	1262:1293	the antibody-mediated activation of NK cells in pregnant women with malaria infection	1262:1346	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	7	45	link	N-linked	1234:1241	arg1	glycans					1243:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	4	46	from	women	672:676	arg1	capacity					631:638	the capacity	627:638	the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3	627:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	46	from	women	672:676	arg1	antibodies					647:656	IgG antibodies	643:656	IgG antibodies from pregnant women, with placental malaria or non-placental malaria,	643:726	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	8	47	theme	antibody-dependent	1397:1414	arg1	activation					1424:1433	antibody-dependent NK cell activation	1397:1433	antibody-dependent NK cell activation	1397:1433	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	6	48	theme	non-pregnant	1150:1161	arg1	controls					1163:1170	healthy non-pregnant controls	1142:1170	healthy non-pregnant controls	1142:1170	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	7	49	with	women	1319:1323	arg1	infection					1338:1346	malaria infection	1330:1346	malaria infection	1330:1346	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	3	50	theme	N-linked	530:537	arg1	glycans					539:545	Fc N-linked glycans	527:545	Fc N-linked glycans	527:545	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	1	51	theme	immune	191:196	arg1	cells					198:202	innate immune cells	184:202	innate immune cells	184:202	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	4	52	theme	IgG	643:645	arg1	antibodies					647:656	IgG antibodies	643:656	IgG antibodies from pregnant women, with placental malaria or non-placental malaria,	643:726	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	53	with	women	672:676	arg1	malaria					694:700	placental malaria	684:700	placental malaria	684:700	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	53	with	women	672:676	arg1	malaria					719:725	non-placental malaria	705:725	non-placental malaria	705:725	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	7	54	theme	antibody-mediated	1266:1282	arg1	activation					1284:1293	the antibody-mediated activation	1262:1293	the antibody-mediated activation of NK cells in pregnant women with malaria infection	1262:1346	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	6	55	theme	Elevated	984:991	arg1	patterns					1025:1032	Elevated anti-inflammatory glycosylation patterns	984:1032	Elevated anti-inflammatory glycosylation patterns of IgG antibodies	984:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	4	56	from	capacity	631:638	arg1	women					672:676	pregnant women	663:676	pregnant women	663:676	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	5	57	theme	Antibody-mediated	825:841	arg1	activation					851:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	5	58	theme	cell	846:849	arg1	activation					851:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation	825:860	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	8	59	theme	placental	1549:1557	arg1	malaria					1559:1565	placental malaria	1549:1565	placental malaria	1549:1565	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	7	60	from	activation	1284:1293	arg1	women					1319:1323	pregnant women	1310:1323	pregnant women with malaria infection	1310:1346	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	4	61	theme	non-placental	705:717	arg1	malaria					719:725	non-placental malaria	705:725	non-placental malaria	705:725	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	0	62	theme	exposed	64:70	arg1	women					81:85	malaria exposed pregnant women	56:85	malaria exposed pregnant women	56:85	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	1	63	theme	antibody-mediated	152:168	arg1	activation					170:179	antibody-mediated activation	152:179	antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity,	152:295	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	6	64	theme	antibodies	1041:1050	arg1	patterns					1025:1032	Elevated anti-inflammatory glycosylation patterns	984:1032	Elevated anti-inflammatory glycosylation patterns of IgG antibodies	984:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	8	65	theme	anti-inflammatory	1367:1383	arg1	glycans					1385:1391	anti-inflammatory glycans	1367:1391	anti-inflammatory glycans	1367:1391	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	5	66	theme	placental	965:973	arg1	malaria					975:981	placental malaria	965:981	placental malaria	965:981	Antibody-mediated NK cell activation was observed in pregnant women with malaria, but no differences were associated with susceptibility to placental malaria.					
33602987	6	67	theme	glycosylation	1011:1023	arg1	patterns					1025:1032	Elevated anti-inflammatory glycosylation patterns	984:1032	Elevated anti-inflammatory glycosylation patterns of IgG antibodies	984:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	8	68	theme	NK	1416:1417	arg1	activation					1424:1433	antibody-dependent NK cell activation	1397:1433	antibody-dependent NK cell activation	1397:1433	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	4	69	theme	cell	741:744	arg1	activation					746:755	NK cell activation	738:755	NK cell activation	738:755	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	7	70	theme	N-linked	1234:1241	arg1	glycans					1243:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	3	71	with	women	559:563	arg1	malaria					570:576	malaria	570:576	malaria	570:576	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	6	72	located	observed	1057:1064	arg1	women					1078:1082	pregnant women	1069:1082	pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls	1069:1170	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	6	72	located	observed	1057:1064	arg2	patterns					1025:1032	Elevated anti-inflammatory glycosylation patterns	984:1032	Elevated anti-inflammatory glycosylation patterns of IgG antibodies	984:1050	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	2	73	theme	immunoglobulin	405:418	arg1	IgG					423:425	IgG	423:425	IgG	423:425	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	2	73	theme	immunoglobulin	405:418	arg1	G					420:420	immunoglobulin G	405:420	immunoglobulin G (IgG) Fc N-linked glycans	405:446	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	1	74	theme	effector	95:102	arg1	responses					104:112	Immune effector responses	88:112	Immune effector responses against Plasmodium falciparum	88:142	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	8	75	theme	pregnant	1452:1459	arg1	women					1461:1465	pregnant women	1452:1465	pregnant women with malaria	1452:1478	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	3	76	theme	natural	487:493	arg1	NK					503:504	NK	503:504	NK	503:504	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	3	76	theme	natural	487:493	arg1	killer					495:500	antibody-mediated natural killer	469:500	antibody-mediated natural killer (NK) cells activation	469:522	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	0	77	theme	killer	40:45	arg1	cells					47:51	natural killer cells	32:51	natural killer cells	32:51	Antibody mediated activation of natural killer cells in malaria exposed pregnant women.					
33602987	1	78	theme	cytokines	318:326	arg1	activation					170:179	antibody-mediated activation	152:179	antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity,	152:295	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	78	theme	cytokines	318:326	arg1	secretion					305:313	the secretion	301:313	the secretion of cytokines and chemokines	301:341	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	79	theme	Fc	222:223	arg1	cytotoxicity					283:294	antibody-dependent cellular cytotoxicity	255:294	antibody-dependent cellular cytotoxicity	255:294	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	79	theme	Fc	222:223	arg1	functions					234:242	Fc effector functions	222:242	Fc effector functions	222:242	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	6	80	theme	pregnant	1069:1076	arg1	women					1078:1082	pregnant women	1069:1082	pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls	1069:1170	Elevated anti-inflammatory glycosylation patterns of IgG antibodies were observed in pregnant women with or without malaria infection, which were not seen in healthy non-pregnant controls.					
33602987	3	81	from	role	460:463	arg1	women					559:563	pregnant women	550:563	pregnant women with malaria	550:576	However, a role for antibody-mediated natural killer (NK) cells activation or Fc N-linked glycans in pregnant women with malaria has not yet been established.					
33602987	1	82	theme	chemokines	332:341	arg1	activation					170:179	antibody-mediated activation	152:179	antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity,	152:295	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	1	82	theme	chemokines	332:341	arg1	secretion					305:313	the secretion	301:313	the secretion of cytokines and chemokines	301:341	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
33602987	7	83	theme	malaria	1330:1336	arg1	infection					1338:1346	malaria infection	1330:1346	malaria infection	1330:1346	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	4	84	dep	antigens	801:808	arg1	antigens					801:808	placental malaria-associated antigens DBL2 and DBL3	772:822	placental malaria-associated antigens DBL2 and DBL3	772:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	84	dep	antigens	801:808	arg1	DBL3					819:822	DBL3	819:822	DBL3	819:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	84	dep	antigens	801:808	arg1	DBL2					810:813	DBL2	810:813	DBL2	810:813	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	8	85	theme	definitive	1483:1492	arg1	role					1494:1497	a definitive role	1481:1497	a definitive role for these antibody features in protecting against placental malaria	1481:1565	Overall, although anti-inflammatory glycans and antibody-dependent NK cell activation were detected in pregnant women with malaria, a definitive role for these antibody features in protecting against placental malaria remains to be proven.					
33602987	2	86	theme	effector	350:357	arg1	functions					359:367	These effector functions	344:367	These effector functions	344:367	These effector functions are regulated by the composition of immunoglobulin G (IgG) Fc N-linked glycans.					
33602987	4	87	theme	malaria-associated	782:799	arg1	antigens					801:808	placental malaria-associated antigens DBL2 and DBL3	772:822	placental malaria-associated antigens DBL2 and DBL3	772:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	87	theme	malaria-associated	782:799	arg1	DBL3					819:822	DBL3	819:822	DBL3	819:822	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	4	87	theme	malaria-associated	782:799	arg1	DBL2					810:813	DBL2	810:813	DBL2	810:813	Herein, we studied the capacity of IgG antibodies from pregnant women, with placental malaria or non-placental malaria, to induce NK cell activation in response to placental malaria-associated antigens DBL2 and DBL3.					
33602987	7	88	theme	anti-inflammatory	1213:1229	arg1	glycans					1243:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	pregnancy-associated anti-inflammatory Fc N-linked glycans	1192:1249	This suggests that pregnancy-associated anti-inflammatory Fc N-linked glycans may dampen the antibody-mediated activation of NK cells in pregnant women with malaria infection.					
33602987	1	89	theme	antibody-dependent	255:272	arg1	cytotoxicity					283:294	antibody-dependent cellular cytotoxicity	255:294	antibody-dependent cellular cytotoxicity	255:294	Immune effector responses against Plasmodium falciparum include antibody-mediated activation of innate immune cells, which can induce Fc effector functions, including antibody-dependent cellular cytotoxicity, and the secretion of cytokines and chemokines.					
32887022	3	0	theme	axis	696:699	arg1	rate					712:715	left ventricular short axis shortening rate	673:715	left ventricular short axis shortening rate (LVFS)	673:722	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	0	theme	axis	696:699	arg1	LVFS					718:721	LVFS	718:721	LVFS	718:721	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	2	1	theme	failure	364:370	arg1	model					349:353	A rat model	343:353	A rat model of heart failure	343:370	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	4	2	theme	brain	851:855	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	2	theme	brain	851:855	arg1	BNP					878:880	BNP	878:880	BNP	878:880	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	2	theme	brain	851:855	arg1	peptide					869:875	brain natriuretic peptide	851:875	brain natriuretic peptide (BNP)	851:881	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	16	3	with	rats	2542:2545	arg1	failure					2558:2564	heart failure	2552:2564	heart failure	2552:2564	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	3	4	theme	end	589:591	arg1	diameter					603:610	left ventricular end diastolic diameter	572:610	left ventricular end diastolic diameter (LVEDD)	572:618	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	4	theme	end	589:591	arg1	LVEDD					613:617	LVEDD	613:617	LVEDD	613:617	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	5	5	theme	6 h	1058:1060	arg1	output					1079:1084	6 h cumulative urine output	1058:1084	6 h cumulative urine output	1058:1084	6 h cumulative urine output was measured by metabolic cage method after administration for 3 weeks.					
32887022	4	6	theme	natriuretic	824:834	arg1	angiotensin					925:935	angiotensin II	925:938	angiotensin II (Ang II)	925:947	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	aldosterone					950:960	aldosterone	950:960	aldosterone (ALD)	950:966	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	I					893:893	troponin I	884:893	troponin I (cTnI)	884:900	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	kinase					912:917	creatine kinase	903:917	creatine kinase (CK)	903:922	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	peptide					869:875	brain natriuretic peptide	851:875	brain natriuretic peptide (BNP)	851:881	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	AQP2					1043:1046	AQP2	1043:1046	AQP2	1043:1046	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	ANP					845:847	ANP	845:847	ANP	845:847	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	6	theme	natriuretic	824:834	arg1	Oxide					1031:1035	Nitric Oxide	1024:1035	Nitric Oxide (NO)	1024:1040	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	16	7	theme	cardiac	2485:2491	arg1	function					2493:2500	cardiac function	2485:2500	cardiac function	2485:2500	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	14	8	dep	LVEDD	1969:1973	arg1	P < 0.01					1976:1983	P < 0.01	1976:1983	P < 0.01	1976:1983	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	14	8	dep	LVEDD	1969:1973	arg1	P < 0.05					1988:1995	P < 0.05	1988:1995	P < 0.05	1988:1995	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	3	9	theme	left	572:575	arg1	diameter					603:610	left ventricular end diastolic diameter	572:610	left ventricular end diastolic diameter (LVEDD)	572:618	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	9	theme	left	572:575	arg1	LVEDD					613:617	LVEDD	613:617	LVEDD	613:617	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	1	10	theme	protective	189:198	arg1	effect					200:205	the protective effect	185:205	the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats	185:305	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	7	11	theme	blot	1289:1292	arg1	method					1294:1299	Western blot method	1281:1299	Western blot method	1281:1299	The expression of AQP2 protein in kidney was detected by Western blot method.					
32887022	7	12	theme	protein	1247:1253	arg1	expression					1228:1237	The expression	1224:1237	The expression of AQP2 protein in kidney	1224:1263	The expression of AQP2 protein in kidney was detected by Western blot method.					
32887022	4	13	used	used	787:790	arg2	method					776:781	ELISA method	770:781	ELISA method	770:781	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	11	14	dep	increased	1701:1709	arg1	P < 0.01					1712:1719	P < 0.01	1712:1719	P < 0.01	1712:1719	The levels of CK, cTnI, NO, ET-1, BNP, ANP, ALD, AngII, Renin, AQP2, AVP and osmotic pressure were significantly increased (P < 0.01).					
32887022	1	15	theme	composition	227:237	arg1	effect					200:205	the protective effect	185:205	the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats	185:305	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	14	16	theme	Descurainiae	1880:1891	arg1	sophia					1893:1898	Descurainiae sophia	1880:1898	Descurainiae sophia	1880:1898	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	0	17	theme	heart	147:151	arg1	failure					153:159	heart failure	147:159	heart failure	147:159	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	3	18	theme	systolic	628:635	arg1	LVESD					647:651	LVESD	647:651	LVESD	647:651	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	18	theme	systolic	628:635	arg1	diameter					637:644	end systolic diameter	624:644	end systolic diameter (LVESD)	624:652	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	1	19	theme	Descurainiae	242:253	arg1	sophia					255:260	Descurainiae sophia	242:260	Descurainiae sophia	242:260	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	16	20	theme	Oligosaccharides	2407:2422	arg1	composition					2424:2434	CONCLUSION Oligosaccharides composition	2396:2434	CONCLUSION Oligosaccharides composition of Descurainiae sophia	2396:2457	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	16	21	theme	heart	2552:2556	arg1	failure					2558:2564	heart failure	2552:2564	heart failure	2552:2564	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	17	22	theme	arginine	2678:2685	arg1	vasopressin					2687:2697	the arginine vasopressin	2674:2697	the arginine vasopressin system	2674:2704	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	1	23	from	effect	200:205	arg1	failure					291:297	doxorubicin-induced heart failure	265:297	doxorubicin-induced heart failure in rats	265:305	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	16	24	theme	sophia	2452:2457	arg1	composition					2424:2434	CONCLUSION Oligosaccharides composition	2396:2434	CONCLUSION Oligosaccharides composition of Descurainiae sophia	2396:2457	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	10	25	dep	decreased	1566:1574	arg1	P < 0.01					1577:1584	P < 0.01	1577:1584	P < 0.01	1577:1584	LVESD and LVEDD were significantly increased (P < 0.01), LVEF and LVFS were significantly decreased (P < 0.01).					
32887022	0	26	with	rats	137:140	arg1	failure					153:159	heart failure	147:159	heart failure	147:159	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	16	27	theme	metabolism	2528:2537	arg1	function					2493:2500	cardiac function	2485:2500	cardiac function	2485:2500	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	16	27	theme	metabolism	2528:2537	arg1	disorder					2510:2517	the disorder	2506:2517	the disorder of water metabolism	2506:2537	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	8	28	theme	morphology	1328:1337	arg1	changes					1306:1312	The changes	1302:1312	The changes of myocardial morphology	1302:1337	The changes of myocardial morphology were observed.					
32887022	14	29	theme	Oligosaccharides	1848:1863	arg1	composition					1865:1875	the Oligosaccharides composition	1844:1875	the Oligosaccharides composition of Descurainiae sophia	1844:1898	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	0	30	theme	heart	91:95	arg1	function					97:104	heart function	91:104	heart function	91:104	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	15	31	from	serum	2224:2228	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	5	32	theme	cage	1112:1115	arg1	method					1117:1122	metabolic cage method	1102:1122	metabolic cage method	1102:1122	6 h cumulative urine output was measured by metabolic cage method after administration for 3 weeks.					
32887022	15	33	theme	protein	2330:2336	arg1	expression					2311:2320	the expression	2307:2320	the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05)	2307:2393	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	17	34	theme	vasopressin	2687:2697	arg1	system					2699:2704	the arginine vasopressin system	2674:2704	the arginine vasopressin system	2674:2704	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	9	35	theme	control	1387:1393	arg1	group					1395:1399	the normal control group	1376:1399	the normal control group	1376:1399	RESULTS Compared with the normal control group, the heart rate of the model group was significantly increased (P < 0.01).					
32887022	15	36	from	Renin	2206:2210	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	14	37	theme	model	1831:1835	arg1	group					1837:1841	the model group	1827:1841	the model group	1827:1841	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	11	38	theme	osmotic	1665:1671	arg1	pressure					1673:1680	osmotic pressure	1665:1680	osmotic pressure	1665:1680	The levels of CK, cTnI, NO, ET-1, BNP, ANP, ALD, AngII, Renin, AQP2, AVP and osmotic pressure were significantly increased (P < 0.01).					
32887022	11	38	theme	osmotic	1665:1671	arg1	CK					1602:1603	CK	1602:1603	CK	1602:1603	The levels of CK, cTnI, NO, ET-1, BNP, ANP, ALD, AngII, Renin, AQP2, AVP and osmotic pressure were significantly increased (P < 0.01).					
32887022	4	39	theme	ELISA	770:774	arg1	method					776:781	ELISA method	770:781	ELISA method	770:781	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	17	40	theme	Oligosaccharides	2567:2582	arg1	composition					2584:2594	Oligosaccharides composition	2567:2594	Oligosaccharides composition of Descurainiae sophia	2567:2617	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	13	41	theme	heart	1780:1784	arg1	HE					1786:1787	The heart HE	1776:1787	The heart HE	1776:1787	The heart HE showed obvious lesions.					
32887022	2	42	theme	male	401:404	arg1	rats					421:424	180-220 g male Sprague-Dawley rats	391:424	180-220 g male Sprague-Dawley rats	391:424	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	0	43	theme	Oligosaccharides	0:15	arg1	composition					17:27	Oligosaccharides composition	0:27	Oligosaccharides composition of Descurainiae sophia	0:50	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	9	44	theme	heart	1406:1410	arg1	rate					1412:1415	the heart rate	1402:1415	the heart rate of the model group	1402:1434	RESULTS Compared with the normal control group, the heart rate of the model group was significantly increased (P < 0.01).					
32887022	2	45	theme	doxorubicin	467:477	arg1	injection					454:462	low-dose intraperitoneal injection	429:462	low-dose intraperitoneal injection of doxorubicin for 6 weeks	429:489	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	15	46	dep	cTnI	2183:2186	arg1	P < 0.05					2282:2289	P < 0.05	2282:2289	P < 0.05	2282:2289	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	46	dep	cTnI	2183:2186	arg1	P < 0.01					2270:2277	P < 0.01	2270:2277	P < 0.01	2270:2277	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	46	dep	cTnI	2183:2186	arg1	urine					2263:2267	the urine	2259:2267	the urine (P < 0.01 or P < 0.05)	2259:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	2	47	theme	low-dose	429:436	arg1	injection					454:462	low-dose intraperitoneal injection	429:462	low-dose intraperitoneal injection of doxorubicin for 6 weeks	429:489	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	0	48	theme	sophia	45:50	arg1	composition					17:27	Oligosaccharides composition	0:27	Oligosaccharides composition of Descurainiae sophia	0:50	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	15	49	theme	increase	2362:2369	arg1	volume					2377:2382	increase urine volume	2362:2382	increase urine volume (P < 0.05)	2362:2393	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	49	theme	increase	2362:2369	arg1	renal					2345:2349	renal	2345:2349	renal	2345:2349	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	49	theme	increase	2362:2369	arg1	P < 0.05					2385:2392	P < 0.05	2385:2392	P < 0.05	2385:2392	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	9	50	theme	model	1424:1428	arg1	group					1430:1434	the model group	1420:1434	the model group	1420:1434	RESULTS Compared with the normal control group, the heart rate of the model group was significantly increased (P < 0.01).					
32887022	10	51	dep	increased	1511:1519	arg1	P < 0.01					1522:1529	P < 0.01	1522:1529	P < 0.01	1522:1529	LVESD and LVEDD were significantly increased (P < 0.01), LVEF and LVFS were significantly decreased (P < 0.01).					
32887022	2	52	theme	heart	358:362	arg1	failure					364:370	heart failure	358:370	heart failure	358:370	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	0	53	theme	anti-heart	59:68	arg1	failure					70:76	anti-heart failure	59:76	anti-heart failure	59:76	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	3	54	theme	short	690:694	arg1	rate					712:715	left ventricular short axis shortening rate	673:715	left ventricular short axis shortening rate (LVFS)	673:722	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	54	theme	short	690:694	arg1	LVFS					718:721	LVFS	718:721	LVFS	718:721	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	1	55	theme	sophia	255:260	arg1	composition					227:237	Oligosaccharides composition	210:237	Oligosaccharides composition of Descurainiae sophia	210:260	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	15	56	from	AVP	2213:2215	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	4	57	theme	pressurization	978:991	arg1	AVP					993:995	arginine pressurization AVP	969:995	arginine pressurization AVP	969:995	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	57	theme	pressurization	978:991	arg1	aldosterone					950:960	aldosterone	950:960	aldosterone (ALD)	950:966	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	3	58	theme	left	673:676	arg1	rate					712:715	left ventricular short axis shortening rate	673:715	left ventricular short axis shortening rate (LVFS)	673:722	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	58	theme	left	673:676	arg1	LVFS					718:721	LVFS	718:721	LVFS	718:721	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	15	59	theme	AQP2in	2252:2257	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	1	60	theme	doxorubicin-induced	265:283	arg1	failure					291:297	doxorubicin-induced heart failure	265:297	doxorubicin-induced heart failure in rats	265:305	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	3	61	dep	weeks	497:501	arg1	administration					520:533	continuous administration	509:533	continuous administration	509:533	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	15	62	theme	pressure	2239:2246	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	17	63	theme	sophia	2612:2617	arg1	composition					2584:2594	Oligosaccharides composition	2567:2594	Oligosaccharides composition of Descurainiae sophia	2567:2617	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	3	64	theme	shortening	701:710	arg1	rate					712:715	left ventricular short axis shortening rate	673:715	left ventricular short axis shortening rate (LVFS)	673:722	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	64	theme	shortening	701:710	arg1	LVFS					718:721	LVFS	718:721	LVFS	718:721	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	65	theme	ventricular	577:587	arg1	diameter					603:610	left ventricular end diastolic diameter	572:610	left ventricular end diastolic diameter (LVEDD)	572:618	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	65	theme	ventricular	577:587	arg1	LVEDD					613:617	LVEDD	613:617	LVEDD	613:617	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	12	66	theme	Urine	1723:1727	arg1	output					1729:1734	Urine output	1723:1734	Urine output	1723:1734	Urine output was significantly decreased (P < 0.01).					
32887022	3	67	theme	ejection	728:735	arg1	LVEF					747:750	LVEF	747:750	LVEF	747:750	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	67	theme	ejection	728:735	arg1	fraction					737:744	ejection fraction	728:744	ejection fraction (LVEF)	728:751	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	7	68	theme	Western	1281:1287	arg1	method					1294:1299	Western blot method	1281:1299	Western blot method	1281:1299	The expression of AQP2 protein in kidney was detected by Western blot method.					
32887022	4	69	theme	atrial	817:822	arg1	angiotensin					925:935	angiotensin II	925:938	angiotensin II (Ang II)	925:947	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	aldosterone					950:960	aldosterone	950:960	aldosterone (ALD)	950:966	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	I					893:893	troponin I	884:893	troponin I (cTnI)	884:900	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	kinase					912:917	creatine kinase	903:917	creatine kinase (CK)	903:922	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	peptide					869:875	brain natriuretic peptide	851:875	brain natriuretic peptide (BNP)	851:881	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	AQP2					1043:1046	AQP2	1043:1046	AQP2	1043:1046	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	ANP					845:847	ANP	845:847	ANP	845:847	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	69	theme	atrial	817:822	arg1	Oxide					1031:1035	Nitric Oxide	1024:1035	Nitric Oxide (NO)	1024:1040	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	15	70	from	levels	2173:2178	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	14	71	theme	heart	1926:1930	arg1	P < 0.05					1938:1945	P < 0.05	1938:1945	P < 0.05	1938:1945	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	14	71	theme	heart	1926:1930	arg1	rate					1932:1935	the heart rate	1922:1935	the heart rate (P < 0.05)	1922:1946	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	15	72	from	cTnI	2183:2186	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	16	73	theme	water	2522:2526	arg1	metabolism					2528:2537	water metabolism	2522:2537	water metabolism	2522:2537	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	3	74	theme	diastolic	593:601	arg1	diameter					603:610	left ventricular end diastolic diameter	572:610	left ventricular end diastolic diameter (LVEDD)	572:618	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	74	theme	diastolic	593:601	arg1	LVEDD					613:617	LVEDD	613:617	LVEDD	613:617	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	1	75	theme	Oligosaccharides	210:225	arg1	composition					227:237	Oligosaccharides composition	210:237	Oligosaccharides composition of Descurainiae sophia	210:260	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	15	76	from	expression	2311:2320	arg1	volume					2377:2382	increase urine volume	2362:2382	increase urine volume (P < 0.05)	2362:2393	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	76	from	expression	2311:2320	arg1	P < 0.01					2351:2358	P < 0.01	2351:2358	P < 0.01	2351:2358	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	76	from	expression	2311:2320	arg1	renal					2345:2349	renal	2345:2349	renal	2345:2349	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	7	77	theme	AQP2	1242:1245	arg1	protein					1247:1253	AQP2 protein	1242:1253	AQP2 protein	1242:1253	The expression of AQP2 protein in kidney was detected by Western blot method.					
32887022	15	78	from	BNP	2189:2191	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	14	79	theme	sophia	1893:1898	arg1	composition					1865:1875	the Oligosaccharides composition	1844:1875	the Oligosaccharides composition of Descurainiae sophia	1844:1898	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	11	80	theme	CK	1602:1603	arg1	levels					1592:1597	The levels	1588:1597	The levels of CK, cTnI, NO, ET-1, BNP, ANP, ALD, AngII, Renin, AQP2, AVP and osmotic pressure	1588:1680	The levels of CK, cTnI, NO, ET-1, BNP, ANP, ALD, AngII, Renin, AQP2, AVP and osmotic pressure were significantly increased (P < 0.01).					
32887022	1	81	from	failure	291:297	arg1	rats					302:305	rats	302:305	rats	302:305	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
32887022	14	82	theme	LVFS	2013:2016	arg1	P < 0.01					2035:2042	P < 0.01	2035:2042	P < 0.01	2035:2042	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	14	82	theme	LVFS	2013:2016	arg1	values					2027:2032	LVFS and LVEF values	2013:2032	LVFS and LVEF values (P < 0.01)	2013:2043	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	3	83	theme	end	624:626	arg1	LVESD					647:651	LVESD	647:651	LVESD	647:651	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	83	theme	end	624:626	arg1	diameter					637:644	end systolic diameter	624:644	end systolic diameter (LVESD)	624:652	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	15	84	dep	improve	2118:2124	arg1	down-regulate					2293:2305	down-regulate	2293:2305	down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05)	2293:2393	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	84	dep	improve	2118:2124	arg1	decrease					2160:2167	decrease	2160:2167	decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2160:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	4	85	theme	troponin	884:891	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	85	theme	troponin	884:891	arg1	cTnI					896:899	cTnI	896:899	cTnI	896:899	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	85	theme	troponin	884:891	arg1	I					893:893	troponin I	884:893	troponin I (cTnI)	884:900	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	9	86	dep	increased	1454:1462	arg1	P < 0.01					1465:1472	P < 0.01	1465:1472	P < 0.01	1465:1472	RESULTS Compared with the normal control group, the heart rate of the model group was significantly increased (P < 0.01).					
32887022	6	87	theme	point	1210:1214	arg1	method					1216:1221	point method	1210:1221	point method	1210:1221	The urine osmotic pressure was measured by freezing point method.					
32887022	15	88	theme	sophia	2091:2096	arg1	composition					2063:2073	Oligosaccharides composition	2046:2073	Oligosaccharides composition of Descurainiae sophia	2046:2096	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	89	theme	AngII	2194:2198	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	90	theme	AQP2	2325:2328	arg1	protein					2330:2336	AQP2 protein	2325:2336	AQP2 protein	2325:2336	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	4	91	theme	natriuretic	857:867	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	91	theme	natriuretic	857:867	arg1	BNP					878:880	BNP	878:880	BNP	878:880	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	91	theme	natriuretic	857:867	arg1	peptide					869:875	brain natriuretic peptide	851:875	brain natriuretic peptide (BNP)	851:881	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	16	92	theme	CONCLUSION	2396:2405	arg1	composition					2424:2434	CONCLUSION Oligosaccharides composition	2396:2434	CONCLUSION Oligosaccharides composition of Descurainiae sophia	2396:2457	CONCLUSION Oligosaccharides composition of Descurainiae sophia can significantly improve cardiac function and the disorder of water metabolism in rats with heart failure.					
32887022	15	93	from	ALD	2201:2203	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	17	94	theme	RAAS	2658:2661	arg1	system					2663:2668	the RAAS system	2654:2668	the RAAS system	2654:2668	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	15	95	theme	BNP	2189:2191	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	8	96	theme	myocardial	1317:1326	arg1	morphology					1328:1337	myocardial morphology	1317:1337	myocardial morphology	1317:1337	The changes of myocardial morphology were observed.					
32887022	4	97	theme	Nitric	1024:1029	arg1	Oxide					1031:1035	Nitric Oxide	1024:1035	Nitric Oxide (NO)	1024:1040	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	97	theme	Nitric	1024:1029	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	97	theme	Nitric	1024:1029	arg1	NO					1038:1039	NO	1038:1039	NO	1038:1039	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	2	98	dep	METHOD	336:341	arg1	established					376:386	established	376:386	was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks	372:489	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	15	99	theme	Oligosaccharides	2046:2061	arg1	composition					2063:2073	Oligosaccharides composition	2046:2073	Oligosaccharides composition of Descurainiae sophia	2046:2096	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	100	theme	cTnI	2183:2186	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	4	101	theme	creatine	903:910	arg1	peptide					836:842	atrial natriuretic peptide	817:842	atrial natriuretic peptide (ANP)	817:848	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	101	theme	creatine	903:910	arg1	kinase					912:917	creatine kinase	903:917	creatine kinase (CK)	903:922	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	101	theme	creatine	903:910	arg1	CK					920:921	CK	920:921	CK	920:921	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	5	102	theme	cumulative	1062:1071	arg1	output					1079:1084	6 h cumulative urine output	1058:1084	6 h cumulative urine output	1058:1084	6 h cumulative urine output was measured by metabolic cage method after administration for 3 weeks.					
32887022	14	103	theme	LVEF	2022:2025	arg1	P < 0.01					2035:2042	P < 0.01	2035:2042	P < 0.01	2035:2042	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	14	103	theme	LVEF	2022:2025	arg1	values					2027:2032	LVFS and LVEF values	2013:2032	LVFS and LVEF values (P < 0.01)	2013:2043	Compared with the model group, the Oligosaccharides composition of Descurainiae sophia significantly reduced the heart rate (P < 0.05), decreased LVESD and LVEDD (P < 0.01 or P < 0.05), and increased LVFS and LVEF values (P < 0.01).					
32887022	13	104	theme	obvious	1796:1802	arg1	lesions					1804:1810	obvious lesions	1796:1810	obvious lesions	1796:1810	The heart HE showed obvious lesions.					
32887022	5	105	theme	metabolic	1102:1110	arg1	method					1117:1122	metabolic cage method	1102:1122	metabolic cage method	1102:1122	6 h cumulative urine output was measured by metabolic cage method after administration for 3 weeks.					
32887022	0	106	theme	water-liquid	110:121	arg1	metabolism					123:132	water-liquid metabolism	110:132	water-liquid metabolism	110:132	Oligosaccharides composition of Descurainiae sophia exerts anti-heart failure by improving heart function and water-liquid metabolism in rats with heart failure.					
32887022	15	107	theme	heart	2153:2157	arg1	damage					2139:2144	pathological damage	2126:2144	pathological damage of the heart	2126:2157	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	9	108	theme	normal	1380:1385	arg1	group					1395:1399	the normal control group	1376:1399	the normal control group	1376:1399	RESULTS Compared with the normal control group, the heart rate of the model group was significantly increased (P < 0.01).					
32887022	2	109	theme	rat	345:347	arg1	model					349:353	A rat model	343:353	A rat model of heart failure	343:370	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	15	110	theme	AVP	2213:2215	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	111	from	pressure	2239:2246	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	2	112	theme	Sprague-Dawley	406:419	arg1	rats					421:424	180-220 g male Sprague-Dawley rats	391:424	180-220 g male Sprague-Dawley rats	391:424	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	15	113	theme	pathological	2126:2137	arg1	damage					2139:2144	pathological damage	2126:2144	pathological damage of the heart	2126:2157	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	114	theme	Renin	2206:2210	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	6	115	theme	osmotic	1168:1174	arg1	pressure					1176:1183	The urine osmotic pressure	1158:1183	The urine osmotic pressure	1158:1183	The urine osmotic pressure was measured by freezing point method.					
32887022	15	116	from	AQP2in	2252:2257	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	2	117	theme	180-220 g	391:399	arg1	rats					421:424	180-220 g male Sprague-Dawley rats	391:424	180-220 g male Sprague-Dawley rats	391:424	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	15	118	theme	urine	2371:2375	arg1	volume					2377:2382	increase urine volume	2362:2382	increase urine volume (P < 0.05)	2362:2393	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	118	theme	urine	2371:2375	arg1	renal					2345:2349	renal	2345:2349	renal	2345:2349	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	118	theme	urine	2371:2375	arg1	P < 0.05					2385:2392	P < 0.05	2385:2392	P < 0.05	2385:2392	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	15	119	theme	ALD	2201:2203	arg1	levels					2173:2178	the levels	2169:2178	the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05)	2169:2290	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	17	120	theme	heart	2632:2636	arg1	failure					2638:2644	anti- heart failure	2626:2644	anti- heart failure	2626:2644	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	2	121	theme	intraperitoneal	438:452	arg1	injection					454:462	low-dose intraperitoneal injection	429:462	low-dose intraperitoneal injection of doxorubicin for 6 weeks	429:489	METHOD A rat model of heart failure was established in 180-220 g male Sprague-Dawley rats by low-dose intraperitoneal injection of doxorubicin for 6 weeks.					
32887022	7	122	from	expression	1228:1237	arg1	kidney					1258:1263	kidney	1258:1263	kidney	1258:1263	The expression of AQP2 protein in kidney was detected by Western blot method.					
32887022	17	123	theme	anti-	2626:2630	arg1	failure					2638:2644	anti- heart failure	2626:2644	anti- heart failure	2626:2644	Oligosaccharides composition of Descurainiae sophia exerts anti- heart failure through the RAAS system and the arginine vasopressin system.					
32887022	9	124	theme	group	1430:1434	arg1	rate					1412:1415	the heart rate	1402:1415	the heart rate of the model group	1402:1434	RESULTS Compared with the normal control group, the heart rate of the model group was significantly increased (P < 0.01).					
32887022	6	125	theme	urine	1162:1166	arg1	pressure					1176:1183	The urine osmotic pressure	1158:1183	The urine osmotic pressure	1158:1183	The urine osmotic pressure was measured by freezing point method.					
32887022	3	126	theme	ventricular	678:688	arg1	rate					712:715	left ventricular short axis shortening rate	673:715	left ventricular short axis shortening rate (LVFS)	673:722	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	3	126	theme	ventricular	678:688	arg1	LVFS					718:721	LVFS	718:721	LVFS	718:721	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	12	127	dep	decreased	1754:1762	arg1	P < 0.01					1765:1772	P < 0.01	1765:1772	P < 0.01	1765:1772	Urine output was significantly decreased (P < 0.01).					
32887022	3	128	theme	continuous	509:518	arg1	administration					520:533	continuous administration	509:533	continuous administration	509:533	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	5	129	theme	urine	1073:1077	arg1	output					1079:1084	6 h cumulative urine output	1058:1084	6 h cumulative urine output	1058:1084	6 h cumulative urine output was measured by metabolic cage method after administration for 3 weeks.					
32887022	15	130	from	AngII	2194:2198	arg1	serum					2224:2228	the serum	2220:2228	the serum	2220:2228	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	4	131	theme	arginine	969:976	arg1	AVP					993:995	arginine pressurization AVP	969:995	arginine pressurization AVP	969:995	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	4	131	theme	arginine	969:976	arg1	aldosterone					950:960	aldosterone	950:960	aldosterone (ALD)	950:966	ELISA method was used to detecte the levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), troponin I (cTnI), creatine kinase (CK), angiotensin II (Ang II), aldosterone (ALD), arginine pressurization AVP, Renin, Endothelin (ET-1), Nitric Oxide (NO), AQP2 in urine.					
32887022	3	132	used	used	557:560	arg2	echocardiography					536:551	echocardiography	536:551	echocardiography	536:551	Four weeks after continuous administration, echocardiography was used to detect left ventricular end diastolic diameter (LVEDD) and end systolic diameter (LVESD) in each group, and left ventricular short axis shortening rate (LVFS) and ejection fraction (LVEF) were calculated.					
32887022	15	133	theme	osmotic	2231:2237	arg1	pressure					2239:2246	osmotic pressure	2231:2246	osmotic pressure	2231:2246	Oligosaccharides composition of Descurainiae sophia could significantly improve pathological damage of the heart, decrease the levels of cTnI, BNP, AngII, ALD, Renin, AVP in the serum, osmotic pressure and AQP2in the urine (P < 0.01 or P < 0.05), down-regulate the expression of AQP2 protein in the renal(P < 0.01), increase urine volume (P < 0.05).					
32887022	1	134	theme	heart	285:289	arg1	failure					291:297	doxorubicin-induced heart failure	265:297	doxorubicin-induced heart failure in rats	265:305	PURPOSE To investigate the protective effect of Oligosaccharides composition of Descurainiae sophia on doxorubicin-induced heart failure in rats, and to study its mechanism.					
34209163	6	0	theme	amylose	1307:1313	arg1	content					1275:1281	content	1275:1281	content	1275:1281	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	0	theme	amylose	1307:1313	arg1	weight					1297:1302	molecular weight	1287:1302	molecular weight	1287:1302	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	3	1	theme	in	673:674	arg1	tests					692:696	in vitro enzymatic tests	673:696	in vitro enzymatic tests	673:696	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	3	2	theme	enzymatic	682:690	arg1	tests					692:696	in vitro enzymatic tests	673:696	in vitro enzymatic tests	673:696	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	2	3	theme	thermal	518:524	arg1	properties					526:535	thermal properties	518:535	thermal properties	518:535	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	7	4	theme	autoclaved	1382:1391	arg1	powders					1393:1399	the autoclaved powders	1378:1399	the autoclaved powders from corn starch and malanga flour	1378:1434	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	6	5	dep	content	1275:1281	arg1	the					1271:1273	the	1271:1273	the	1271:1273	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	1	6	theme	Xanthosoma	231:240	arg1	flour					224:228	malanga flour	216:228	malanga flour (Xanthosoma sagittifolium)	216:255	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	1	6	theme	Xanthosoma	231:240	arg1	sagittifolium					242:254	Xanthosoma sagittifolium	231:254	Xanthosoma sagittifolium	231:254	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	7	7	from	starch	1411:1416	arg1	powders					1393:1399	the autoclaved powders	1378:1399	the autoclaved powders from corn starch and malanga flour	1378:1434	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	3	8	dep	in	673:674	arg1	vitro					676:680	vitro	676:680	vitro	676:680	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	2	9	theme	attributes	502:511	arg1	evaluation					456:465	the evaluation	452:465	the evaluation of the chemical composition, color attributes, and thermal properties	452:535	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	1	10	theme	hydrothermal	321:332	arg1	treatment					334:342	the hydrothermal treatment	317:342	the hydrothermal treatment of autoclaving	317:357	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	6	11	theme	morphological	1201:1213	arg1	features					1215:1222	their thermal and morphological features	1183:1222	their thermal and morphological features	1183:1222	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	0	12	dep	Xanthosoma	101:110	arg1	sagittifolium					112:124	Xanthosoma sagittifolium	101:124	Xanthosoma sagittifolium	101:124	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	2	13	theme	color	496:500	arg1	attributes					502:511	color attributes	496:511	color attributes	496:511	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	4	14	theme	commercial	701:710	arg1	sample					724:729	A commercial corn starch sample	699:729	A commercial corn starch sample	699:729	A commercial corn starch sample was used to produce RS3 and utilized to compare all of the analyses.					
34209163	5	15	theme	commercial	919:928	arg1	starch					935:940	the commercial corn starch	915:940	the commercial corn starch	915:940	The results showed that native malanga flour behaved differently in most of the evaluations performed, compared to the commercial corn starch.					
34209163	6	16	from	similar	1172:1178	arg1	features					1215:1222	their thermal and morphological features	1183:1222	their thermal and morphological features	1183:1222	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	16	from	similar	1172:1178	arg1	both					1319:1322	both	1319:1322	both	1319:1322	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	16	from	similar	1172:1178	arg1	samples					1331:1337	the samples	1327:1337	the samples	1327:1337	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	17	from	both	1319:1322	arg1	samples					1115:1121	the RS3 samples	1107:1121	the RS3 samples obtained from malanga flour and corn starch	1107:1165	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	17	from	both	1319:1322	arg1	similar					1172:1178	similar	1172:1178	similar	1172:1178	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	18	theme	thermal	1189:1195	arg1	features					1215:1222	their thermal and morphological features	1183:1222	their thermal and morphological features	1183:1222	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	5	19	theme	corn	930:933	arg1	starch					935:940	the commercial corn starch	915:940	the commercial corn starch	915:940	The results showed that native malanga flour behaved differently in most of the evaluations performed, compared to the commercial corn starch.					
34209163	2	20	theme	physicochemical	364:378	arg1	characterization					380:395	The physicochemical characterization	360:395	The physicochemical characterization of RS3 made from malanga flour	360:426	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	1	21	theme	autoclaving	347:357	arg1	treatment					334:342	the hydrothermal treatment	317:342	the hydrothermal treatment of autoclaving	317:357	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	6	22	from	similarities	1255:1266	arg1	content					1275:1281	content	1275:1281	content	1275:1281	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	22	from	similarities	1255:1266	arg1	weight					1297:1302	molecular weight	1287:1302	molecular weight	1287:1302	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	0	23	dep	Autoclaving	80:90	arg1	Flour					127:131	Malanga (Xanthosoma sagittifolium) Flour	92:131	Malanga (Xanthosoma sagittifolium) Flour	92:131	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	1	24	theme	resistant	179:187	arg1	starch					189:194	resistant starch type III (RS3)	179:209	resistant starch type III (RS3)	179:209	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	0	25	theme	Physicochemical	0:14	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization of Resistant Starch Type-III (RS3)	0:66	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	5	26	dep	behaved	845:851	arg1	compared					903:910	compared	903:910	compared to the commercial corn starch	903:940	The results showed that native malanga flour behaved differently in most of the evaluations performed, compared to the commercial corn starch.					
34209163	7	27	dep	similar	1441:1447	arg1	similar					1441:1447	similar	1441:1447	similar	1441:1447	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	27	dep	similar	1441:1447	arg1	%					1453:1453	≈89%	1450:1453	≈89%	1450:1453	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	27	dep	similar	1441:1447	arg1	yields					1357:1362	the yields	1353:1362	the yields for obtaining the autoclaved powders from corn starch and malanga flour	1353:1434	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	28	theme	corn	1406:1409	arg1	starch					1411:1416	corn starch	1406:1416	corn starch	1406:1416	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	29	theme	malanga	1422:1428	arg1	flour					1430:1434	malanga flour	1422:1434	malanga flour	1422:1434	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	30	theme	adequate	1546:1553	arg1	yields					1555:1560	adequate yields	1546:1560	adequate yields	1546:1560	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	5	31	theme	native	824:829	arg1	flour					839:843	native malanga flour	824:843	native malanga flour	824:843	The results showed that native malanga flour behaved differently in most of the evaluations performed, compared to the commercial corn starch.					
34209163	0	32	theme	Resistant	36:44	arg1	Starch					46:51	Resistant Starch Type-III (RS3)	36:66	Resistant Starch Type-III (RS3)	36:66	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	6	33	theme	malanga	1137:1143	arg1	flour					1145:1149	malanga flour	1137:1149	malanga flour	1137:1149	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	34	theme	corn	1155:1158	arg1	starch					1160:1165	corn starch	1155:1165	corn starch	1155:1165	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	4	35	theme	starch	717:722	arg1	sample					724:729	A commercial corn starch sample	699:729	A commercial corn starch sample	699:729	A commercial corn starch sample was used to produce RS3 and utilized to compare all of the analyses.					
34209163	4	36	used	utilized	759:766	arg2	sample					724:729	A commercial corn starch sample	699:729	A commercial corn starch sample	699:729	A commercial corn starch sample was used to produce RS3 and utilized to compare all of the analyses.					
34209163	6	37	theme	minor	995:999	arg1	components					1001:1010	minor components	995:1010	minor components that could interfere with the physicochemical and functional properties of the flour	995:1095	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	2	38	theme	malanga	414:420	arg1	flour					422:426	malanga flour	414:426	malanga flour	414:426	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	7	39	theme	raw	1510:1512	arg1	material					1514:1521	an attractive raw material	1496:1521	an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research	1496:1605	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	39	theme	raw	1510:1512	arg1	flour					1487:1491	the malanga flour	1475:1491	the malanga flour	1475:1491	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	2	40	theme	properties	526:535	arg1	evaluation					456:465	the evaluation	452:465	the evaluation of the chemical composition, color attributes, and thermal properties	452:535	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	3	41	theme	starch	613:618	arg1	contents					555:562	the contents	551:562	the contents of the total starch, available starch, resistant starch, and retrograded resistant starch	551:652	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	4	42	theme	corn	712:715	arg1	sample					724:729	A commercial corn starch sample	699:729	A commercial corn starch sample	699:729	A commercial corn starch sample was used to produce RS3 and utilized to compare all of the analyses.					
34209163	1	43	theme	unconventional	264:277	arg1	source					279:284	an unconventional source	261:284	an unconventional source of starch	261:294	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	0	44	theme	Corn	137:140	arg1	Starch					142:147	Corn Starch	137:147	Corn Starch	137:147	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	6	45	theme	components	1001:1010	arg1	presence					983:990	the presence	979:990	the presence of minor components that could interfere with the physicochemical and functional properties of the flour	979:1095	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	2	46	theme	RS3	400:402	arg1	characterization					380:395	The physicochemical characterization	360:395	The physicochemical characterization of RS3 made from malanga flour	360:426	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	0	47	theme	Starch	46:51	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization of Resistant Starch Type-III (RS3)	0:66	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	1	48	dep	starch	189:194	arg1	type					196:199	type III	196:203	resistant starch type III (RS3)	179:209	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	1	48	dep	starch	189:194	arg1	RS3					206:208	RS3	206:208	RS3	206:208	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	7	49	theme	attractive	1499:1508	arg1	material					1514:1521	an attractive raw material	1496:1521	an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research	1496:1605	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	49	theme	attractive	1499:1508	arg1	flour					1487:1491	the malanga flour	1475:1491	the malanga flour	1475:1491	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	3	50	theme	resistant	603:611	arg1	starch					613:618	resistant starch	603:618	resistant starch	603:618	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	1	51	theme	starch	289:294	arg1	source					279:284	an unconventional source	261:284	an unconventional source of starch	261:294	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	3	52	theme	resistant	637:645	arg1	starch					647:652	retrograded resistant starch	625:652	retrograded resistant starch	625:652	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	0	53	dep	Starch	46:51	arg1	Type-III					53:60	Type-III	53:60	Resistant Starch Type-III (RS3)	36:66	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	0	53	dep	Starch	46:51	arg1	RS3					63:65	RS3	63:65	RS3	63:65	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
34209163	7	54	from	flour	1430:1434	arg1	powders					1393:1399	the autoclaved powders	1378:1399	the autoclaved powders from corn starch and malanga flour	1378:1434	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	4	55	used	used	735:738	arg2	sample					724:729	A commercial corn starch sample	699:729	A commercial corn starch sample	699:729	A commercial corn starch sample was used to produce RS3 and utilized to compare all of the analyses.					
34209163	3	56	theme	starch	647:652	arg1	contents					555:562	the contents	551:562	the contents of the total starch, available starch, resistant starch, and retrograded resistant starch	551:652	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	3	57	theme	available	585:593	arg1	starch					595:600	available starch	585:600	available starch	585:600	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	3	58	theme	total	571:575	arg1	starch					577:582	the total starch	567:582	the total starch	567:582	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	6	59	from	features	1215:1222	arg1	samples					1115:1121	the RS3 samples	1107:1121	the RS3 samples obtained from malanga flour and corn starch	1107:1165	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	59	from	features	1215:1222	arg1	similar					1172:1178	similar	1172:1178	similar	1172:1178	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	3	60	theme	starch	595:600	arg1	contents					555:562	the contents	551:562	the contents of the total starch, available starch, resistant starch, and retrograded resistant starch	551:652	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	6	61	theme	functional	1062:1071	arg1	properties					1073:1082	the physicochemical and functional properties	1038:1082	the physicochemical and functional properties of the flour	1038:1095	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	62	theme	molecular	1287:1295	arg1	weight					1297:1302	molecular weight	1287:1302	molecular weight	1287:1302	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	7	63	theme	malanga	1479:1485	arg1	material					1514:1521	an attractive raw material	1496:1521	an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research	1496:1605	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	7	63	theme	malanga	1479:1485	arg1	flour					1487:1491	the malanga flour	1475:1491	the malanga flour	1475:1491	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	2	64	theme	composition	483:493	arg1	evaluation					456:465	the evaluation	452:465	the evaluation of the chemical composition, color attributes, and thermal properties	452:535	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	3	65	theme	retrograded	625:635	arg1	starch					647:652	retrograded resistant starch	625:652	retrograded resistant starch	625:652	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	3	66	theme	starch	577:582	arg1	contents					555:562	the contents	551:562	the contents of the total starch, available starch, resistant starch, and retrograded resistant starch	551:652	In addition, the contents of the total starch, available starch, resistant starch, and retrograded resistant starch were determined by in vitro enzymatic tests.					
34209163	5	67	theme	malanga	831:837	arg1	flour					839:843	native malanga flour	824:843	native malanga flour	824:843	The results showed that native malanga flour behaved differently in most of the evaluations performed, compared to the commercial corn starch.					
34209163	6	68	theme	RS3	1111:1113	arg1	samples					1115:1121	the RS3 samples	1107:1121	the RS3 samples obtained from malanga flour and corn starch	1107:1165	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	68	theme	RS3	1111:1113	arg1	similar					1172:1178	similar	1172:1178	similar	1172:1178	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	1	69	theme	malanga	216:222	arg1	flour					224:228	malanga flour	216:228	malanga flour (Xanthosoma sagittifolium)	216:255	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	1	69	theme	malanga	216:222	arg1	sagittifolium					242:254	Xanthosoma sagittifolium	231:254	Xanthosoma sagittifolium	231:254	The feasibility of obtaining resistant starch type III (RS3) from malanga flour (Xanthosoma sagittifolium), as an unconventional source of starch, was evaluated using the hydrothermal treatment of autoclaving.					
34209163	2	70	theme	chemical	474:481	arg1	composition					483:493	the chemical composition	470:493	the chemical composition	470:493	The physicochemical characterization of RS3 made from malanga flour was carried out through the evaluation of the chemical composition, color attributes, and thermal properties.					
34209163	6	71	theme	flour	1091:1095	arg1	properties					1073:1082	the physicochemical and functional properties	1038:1082	the physicochemical and functional properties of the flour	1038:1095	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	6	72	theme	physicochemical	1042:1056	arg1	properties					1073:1082	the physicochemical and functional properties	1038:1082	the physicochemical and functional properties of the flour	1038:1095	These results could be explained by the presence of minor components that could interfere with the physicochemical and functional properties of the flour; however, the RS3 samples obtained from malanga flour and corn starch were similar in their thermal and morphological features, which may be related to their similarities in the content and molecular weight of amylose, in both of the samples.					
34209163	7	73	theme	subsequent	1587:1596	arg1	research					1598:1605	the subsequent research	1583:1605	the subsequent research	1583:1605	Furthermore, the yields for obtaining the autoclaved powders from corn starch and malanga flour were similar (≈89%), which showed that the malanga flour is an attractive raw material for obtaining RS3 with adequate yields, to be considered in the subsequent research.					
34209163	0	74	theme	Malanga	92:98	arg1	Flour					127:131	Malanga (Xanthosoma sagittifolium) Flour	92:131	Malanga (Xanthosoma sagittifolium) Flour	92:131	Physicochemical Characterization of Resistant Starch Type-III (RS3) Obtained by Autoclaving Malanga (Xanthosoma sagittifolium) Flour and Corn Starch.					
33811299	7	0	theme	surface	950:956	arg1	morphology					958:967	surface morphology	950:967	surface morphology	950:967	Coated tablets were also characterized for surface morphology and coating uniformity by near infrared hyperspectroscopy.					
33811299	10	1	theme	dependent	1435:1443	arg1	variables					1445:1453	the dependent variables	1431:1453	the dependent variables for both the blends	1431:1473	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	9	2	theme	p	1201:1201	arg1	<					1203:1203	p < 0.05	1201:1208	p < 0.05	1201:1208	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	9	3	contain	had	1170:1172	arg1	X1					1167:1168	X1	1167:1168	X1	1167:1168	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	9	3	contain	had	1170:1172	arg2	effect					1211:1216	statistically significant (p < 0.05) effect	1174:1216	statistically significant (p < 0.05) effect	1174:1216	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	4	4	dep	%	690:690	arg1	7.5					679:681	7.5	679:681	7.5	679:681	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	4	dep	%	690:690	arg1	10					688:689	10	688:689	10	688:689	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	1	5	theme	research	189:196	arg1	objective					168:176	The objective	164:176	The objective of current research	164:196	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	4	6	theme	weight	692:697	arg1	gain					699:702	approximately 5, 7.5, and 10% weight gain	662:702	approximately 5, 7.5, and 10% weight gain	662:702	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	7	theme	Diclofenac	554:563	arg1	tablets					577:583	Diclofenac sodium core tablets	554:583	Diclofenac sodium core tablets	554:583	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	7	8	theme	Coated	907:912	arg1	tablets					914:920	Coated tablets	907:920	Coated tablets	907:920	Coated tablets were also characterized for surface morphology and coating uniformity by near infrared hyperspectroscopy.					
33811299	11	9	theme	independent	1504:1514	arg1	experiment					1516:1525	independent experiment	1504:1525	independent experiment	1504:1525	The models were verified by independent experiment.					
33811299	9	10	dep	significant	1188:1198	arg1	<					1203:1203	p < 0.05	1201:1208	p < 0.05	1201:1208	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	0	11	theme	acetate	145:151	arg1	phthalate					153:161	cellulose acetate phthalate	135:161	cellulose acetate phthalate	135:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	9	12	theme	CA	1236:1237	arg1	320S/C-A-P					1239:1248	CA 320S/C-A-P	1236:1248	CA 320S/C-A-P	1236:1248	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	1	13	dep	acetate	283:289	arg1	320S					295:298	CA 320S	292:298	CA 320S	292:298	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	1	13	dep	acetate	283:289	arg1	398-10					306:311	CA 398-10	303:311	CA 398-10	303:311	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	9	14	from	X2	1300:1301	arg1	398-10/C-A-P					1309:1320	CA 398-10/C-A-P	1306:1320	CA 398-10/C-A-P	1306:1320	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	8	15	theme	CA	1109:1110	arg1	blends					1125:1130	CA 398-10/C-A-P blends	1109:1130	CA 398-10/C-A-P blends	1109:1130	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	1	16	theme	cellulose	318:326	arg1	blends					354:359	cellulose acetate phthalate (C-A-P) blends	318:359	cellulose acetate phthalate (C-A-P) blends	318:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	5	17	theme	CA	717:718	arg1	content					727:733	CA 398-10 content	717:733	CA 398-10 content	717:733	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	1	18	theme	phthalate	336:344	arg1	blends					354:359	cellulose acetate phthalate (C-A-P) blends	318:359	cellulose acetate phthalate (C-A-P) blends	318:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	2	19	from	percent	423:429	arg1	blend					467:471	the blend	463:471	the blend	463:471	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	1	20	theme	C-A-P	347:351	arg1	blends					354:359	cellulose acetate phthalate (C-A-P) blends	318:359	cellulose acetate phthalate (C-A-P) blends	318:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	12	21	theme	%	1622:1622	arg1	residual					1607:1614	residual	1607:1614	residual	1607:1614	Model predicted and empirical values of Y1, Y2, and Y3 were close with maximum residual of 7.0%.					
33811299	8	22	dep	affected	1073:1080	arg1	<					1065:1065	p < 0.05	1063:1070	p < 0.05	1063:1070	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	0	23	theme	cellulose	113:121	arg1	acetate					123:129	cellulose acetate	113:129	cellulose acetate	113:129	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	13	24	theme	weight	1728:1733	arg1	composition					1680:1690	composition	1680:1690	composition of blend, polymer type, and coating weight	1680:1733	In conclusion, dissolution can be modulated by varying composition of blend, polymer type, and coating weight.					
33811299	0	25	theme	predictive	50:59	arg1	models					73:78	predictive dissolution models	50:78	predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate	50:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	5	26	theme	relative	801:808	arg1	%					799:799	25.0-50.0%	790:799	25.0-50.0% relative to C-A-P	790:817	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	4	27	theme	CA	618:619	arg1	320S					621:624	CA 320S	618:624	CA 320S	618:624	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	10	28	theme	p	1386:1386	arg1	<					1388:1388	p < 0.05	1386:1393	p < 0.05	1386:1393	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	0	29	theme	Coating	0:6	arg1	characterization					8:23	Coating characterization	0:23	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.	0:162	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	9	30	dep	statistically	1263:1275	arg1	<					1280:1280	p < 0.05	1278:1285	p < 0.05	1278:1285	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	7	31	theme	near	995:998	arg1	hyperspectroscopy					1009:1025	near infrared hyperspectroscopy	995:1025	near infrared hyperspectroscopy	995:1025	Coated tablets were also characterized for surface morphology and coating uniformity by near infrared hyperspectroscopy.					
33811299	4	32	theme	core	572:575	arg1	tablets					577:583	Diclofenac sodium core tablets	554:583	Diclofenac sodium core tablets	554:583	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	2	33	theme	CA	445:446	arg1	X2					456:457	X2	456:457	X2	456:457	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	2	33	theme	CA	445:446	arg1	398-10					448:453	CA 398-10	445:453	CA 398-10 (X2)	445:458	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	1	34	theme	CA	303:304	arg1	398-10					306:311	CA 398-10	303:311	CA 398-10	303:311	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	12	35	theme	Y3	1580:1581	arg1	values					1558:1563	empirical values	1548:1563	empirical values of Y1, Y2, and Y3	1548:1581	Model predicted and empirical values of Y1, Y2, and Y3 were close with maximum residual of 7.0%.					
33811299	2	36	theme	320S	437:440	arg1	percent					423:429	percent	423:429	percent of CA 320S or CA 398-10 (X2)	423:458	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	2	36	theme	320S	437:440	arg1	percent					406:412	coating percent	398:412	coating percent (X1)	398:417	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	2	36	theme	320S	437:440	arg1	X1					415:416	X1	415:416	X1	415:416	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	13	37	theme	polymer	1702:1708	arg1	type					1710:1713	polymer type	1702:1713	polymer type	1702:1713	In conclusion, dissolution can be modulated by varying composition of blend, polymer type, and coating weight.					
33811299	4	38	dep	gain	699:702	arg1	%					690:690	%	690:690	%	690:690	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	10	39	theme	variables	1418:1426	arg1	effect					1396:1401	statistically significant (p < 0.05) effect	1359:1401	statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends	1359:1473	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	1	40	theme	dissolution	227:237	arg1	prediction					239:248	dissolution prediction	227:248	dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends	227:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	10	41	from	effect	1396:1401	arg1	variables					1445:1453	the dependent variables	1431:1453	the dependent variables for both the blends	1431:1473	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	13	42	theme	blend	1695:1699	arg1	composition					1680:1690	composition	1680:1690	composition of blend, polymer type, and coating weight	1680:1733	In conclusion, dissolution can be modulated by varying composition of blend, polymer type, and coating weight.					
33811299	10	43	dep	significant	1373:1383	arg1	<					1388:1388	p < 0.05	1386:1393	p < 0.05	1386:1393	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	1	44	theme	cellulose	273:281	arg1	acetate					283:289	cellulose acetate	273:289	cellulose acetate (CA 320S or CA 398-10)	273:312	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	9	45	theme	p	1278:1278	arg1	<					1280:1280	p < 0.05	1278:1285	p < 0.05	1278:1285	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	3	46	theme	Dependent	474:482	arg1	variables					484:492	Dependent variables	474:492	Dependent variables selected	474:501	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	1	47	theme	current	181:187	arg1	research					189:196	current research	181:196	current research	181:196	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	0	48	theme	dissolution	61:71	arg1	models					73:78	predictive dissolution models	50:78	predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate	50:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	10	49	theme	variance	1335:1342	arg1	Analysis					1323:1330	Analysis	1323:1330	Analysis of variance	1323:1342	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	5	50	theme	CA	705:706	arg1	320S					708:711	CA 320S	705:711	CA 320S	705:711	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	8	51	theme	p	1063:1063	arg1	<					1065:1065	p < 0.05	1063:1070	p < 0.05	1063:1070	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	0	52	theme	cellulose	135:143	arg1	phthalate					153:161	cellulose acetate phthalate	135:161	cellulose acetate phthalate	135:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	10	53	theme	significant	1373:1383	arg1	effect					1396:1401	statistically significant (p < 0.05) effect	1359:1401	statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends	1359:1473	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	0	54	theme	phthalate	153:161	arg1	blends					103:108	blends	103:108	blends of cellulose acetate and cellulose acetate phthalate	103:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	3	55	from	dissolution	508:518	arg1	8					531:531	8	531:531	8	531:531	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	3	55	from	dissolution	508:518	arg1	h					546:546	24 h	543:546	24 h (Y3)	543:551	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	3	55	from	dissolution	508:518	arg1	1					523:523	1	523:523	1	523:523	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	3	55	from	dissolution	508:518	arg1	Y2					534:535	Y2	534:535	Y2	534:535	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	3	55	from	dissolution	508:518	arg1	Y3					549:550	Y3	549:550	Y3	549:550	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	3	55	from	dissolution	508:518	arg1	Y1					526:527	Y1	526:527	Y1	526:527	Dependent variables selected were dissolution in 1 (Y1), 8 (Y2), and 24 h (Y3).					
33811299	5	56	theme	corresponding	742:754	arg1	blends					756:761	the corresponding blends	738:761	the corresponding blends	738:761	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	8	57	theme	CA	1091:1092	arg1	320S/C-A-P					1094:1103	CA 320S/C-A-P	1091:1103	CA 320S/C-A-P	1091:1103	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	0	58	theme	tablets	83:89	arg1	hyperspectroscopy					28:44	hyperspectroscopy	28:44	hyperspectroscopy	28:44	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	0	58	theme	tablets	83:89	arg1	models					73:78	predictive dissolution models	50:78	predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate	50:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	5	59	from	320S	708:711	arg1	blends					756:761	the corresponding blends	738:761	the corresponding blends	738:761	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	4	60	theme	sodium	565:570	arg1	tablets					577:583	Diclofenac sodium core tablets	554:583	Diclofenac sodium core tablets	554:583	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	1	61	theme	acetate	328:334	arg1	blends					354:359	cellulose acetate phthalate (C-A-P) blends	318:359	cellulose acetate phthalate (C-A-P) blends	318:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	5	62	theme	398-10	720:725	arg1	content					727:733	CA 398-10 content	717:733	CA 398-10 content	717:733	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	2	63	theme	398-10	448:453	arg1	percent					423:429	percent	423:429	percent of CA 320S or CA 398-10 (X2)	423:458	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	2	63	theme	398-10	448:453	arg1	percent					406:412	coating percent	398:412	coating percent (X1)	398:417	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	2	63	theme	398-10	448:453	arg1	X1					415:416	X1	415:416	X1	415:416	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	8	64	theme	398-10/C-A-P	1112:1123	arg1	blends					1125:1130	CA 398-10/C-A-P blends	1109:1130	CA 398-10/C-A-P blends	1109:1130	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	4	65	from	gain	699:702	arg1	blend					602:606	blend	602:606	blend of either CA 320S and C-A-P	602:634	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	65	from	gain	699:702	arg1	C-A-P					653:657	C-A-P	653:657	C-A-P	653:657	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	65	from	gain	699:702	arg1	398-10					642:647	CA 398-10	639:647	CA 398-10	639:647	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	5	66	from	content	727:733	arg1	blends					756:761	the corresponding blends	738:761	the corresponding blends	738:761	CA 320S and CA 398-10 content in the corresponding blends varied from 33.3-66.7% and 25.0-50.0% relative to C-A-P, respectively.					
33811299	2	67	from	percent	406:412	arg1	blend					467:471	the blend	463:471	the blend	463:471	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	4	68	theme	C-A-P	630:634	arg1	blend					602:606	blend	602:606	blend of either CA 320S and C-A-P	602:634	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	68	theme	C-A-P	630:634	arg1	C-A-P					653:657	C-A-P	653:657	C-A-P	653:657	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	68	theme	C-A-P	630:634	arg1	398-10					642:647	CA 398-10	639:647	CA 398-10	639:647	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	8	69	theme	coated	1132:1137	arg1	tablets					1139:1145	coated tablets	1132:1145	coated tablets	1132:1145	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	0	70	theme	acetate	123:129	arg1	blends					103:108	blends	103:108	blends of cellulose acetate and cellulose acetate phthalate	103:161	Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.					
33811299	13	71	theme	coating	1720:1726	arg1	weight					1728:1733	coating weight	1720:1733	coating weight	1720:1733	In conclusion, dissolution can be modulated by varying composition of blend, polymer type, and coating weight.					
33811299	4	72	theme	CA	639:640	arg1	398-10					642:647	CA 398-10	639:647	CA 398-10	639:647	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	7	73	theme	infrared	1000:1007	arg1	hyperspectroscopy					1009:1025	near infrared hyperspectroscopy	995:1025	near infrared hyperspectroscopy	995:1025	Coated tablets were also characterized for surface morphology and coating uniformity by near infrared hyperspectroscopy.					
33811299	6	74	theme	USP	890:892	arg1	apparatus					894:902	USP apparatus 2	890:904	USP apparatus 2	890:904	Dissolution was performed in phosphate buffer 6.8 using USP apparatus 2.					
33811299	2	75	theme	Independent	362:372	arg1	variables					374:382	Independent variables	362:382	Independent variables selected	362:391	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	1	76	theme	CA	292:293	arg1	320S					295:298	CA 320S	292:298	CA 320S	292:298	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	9	77	theme	CA	1306:1307	arg1	398-10/C-A-P					1309:1320	CA 398-10/C-A-P	1306:1320	CA 398-10/C-A-P	1306:1320	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	4	78	theme	320S	621:624	arg1	blend					602:606	blend	602:606	blend of either CA 320S and C-A-P	602:634	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	78	theme	320S	621:624	arg1	C-A-P					653:657	C-A-P	653:657	C-A-P	653:657	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	4	78	theme	320S	621:624	arg1	398-10					642:647	CA 398-10	639:647	CA 398-10	639:647	Diclofenac sodium core tablets were coated with blend of either CA 320S and C-A-P or CA 398-10 and C-A-P at approximately 5, 7.5, and 10% weight gain.					
33811299	7	79	theme	coating	973:979	arg1	uniformity					981:990	coating uniformity	973:990	coating uniformity	973:990	Coated tablets were also characterized for surface morphology and coating uniformity by near infrared hyperspectroscopy.					
33811299	6	80	theme	phosphate	863:871	arg1	buffer					873:878	phosphate buffer 6.8	863:882	phosphate buffer 6.8	863:882	Dissolution was performed in phosphate buffer 6.8 using USP apparatus 2.					
33811299	2	81	theme	coating	398:404	arg1	percent					406:412	coating percent	398:412	coating percent (X1)	398:417	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	2	81	theme	coating	398:404	arg1	X1					415:416	X1	415:416	X1	415:416	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	9	82	theme	other	1155:1159	arg1	hand					1161:1164	the other hand	1151:1164	the other hand	1151:1164	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
33811299	12	83	theme	Y1	1568:1569	arg1	values					1558:1563	empirical values	1548:1563	empirical values of Y1, Y2, and Y3	1548:1581	Model predicted and empirical values of Y1, Y2, and Y3 were close with maximum residual of 7.0%.					
33811299	2	84	theme	CA	434:435	arg1	320S					437:440	CA 320S	434:440	CA 320S	434:440	Independent variables selected were coating percent (X1) and percent of CA 320S or CA 398-10 (X2) in the blend.					
33811299	13	85	theme	type	1710:1713	arg1	composition					1680:1690	composition	1680:1690	composition of blend, polymer type, and coating weight	1680:1733	In conclusion, dissolution can be modulated by varying composition of blend, polymer type, and coating weight.					
33811299	12	86	with	close	1588:1592	arg1	residual					1607:1614	residual	1607:1614	residual	1607:1614	Model predicted and empirical values of Y1, Y2, and Y3 were close with maximum residual of 7.0%.					
33811299	12	87	theme	Y2	1572:1573	arg1	values					1558:1563	empirical values	1548:1563	empirical values of Y1, Y2, and Y3	1548:1581	Model predicted and empirical values of Y1, Y2, and Y3 were close with maximum residual of 7.0%.					
33811299	8	88	dep	320S/C-A-P	1094:1103	arg1	tablets					1139:1145	coated tablets	1132:1145	coated tablets	1132:1145	Y1, Y2, and Y3 were statistically (p < 0.05) affected by X2 in CA 320S/C-A-P and CA 398-10/C-A-P blends coated tablets.					
33811299	12	89	theme	empirical	1548:1556	arg1	values					1558:1563	empirical values	1548:1563	empirical values of Y1, Y2, and Y3	1548:1581	Model predicted and empirical values of Y1, Y2, and Y3 were close with maximum residual of 7.0%.					
33811299	10	90	theme	studied	1410:1416	arg1	variables					1418:1426	the studied variables	1406:1426	the studied variables	1406:1426	Analysis of variance also indicated statistically significant (p < 0.05) effect of the studied variables on the dependent variables for both the blends.					
33811299	1	91	theme	prediction	239:248	arg1	models					217:222	the models	213:222	the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends	213:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	1	92	theme	tablets	253:259	arg1	prediction					239:248	dissolution prediction	227:248	dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends	227:359	The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends.					
33811299	9	93	theme	significant	1188:1198	arg1	effect					1211:1216	statistically significant (p < 0.05) effect	1174:1216	statistically significant (p < 0.05) effect	1174:1216	On the other hand, X1 had statistically significant (p < 0.05) effect only on the Y3 in CA 320S/C-A-P while Y1 was statistically (p < 0.05) affected by X2 in CA 398-10/C-A-P.					
32041744	0	0	theme	intervention	75:86	arg1	efficacy					53:60	the efficacy	49:60	the efficacy of prebiotic intervention in obese patients	49:104	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	4	1	theme	microbial	498:506	arg1	diversity					508:516	microbial diversity	498:516	microbial diversity	498:516	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	5	2	theme	Hum-ob	661:666	arg1	mice					668:671	Hum-ob mice	661:671	Hum-ob mice	661:671	Hum-ob mice were fed with a high-fat diet and treated with inulin.					
32041744	7	3	theme	obese	987:991	arg1	individuals					993:1003	different obese individuals	977:1003	different obese individuals	977:1003	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	8	4	theme	hum-ob	1309:1314	arg1	mice					1316:1319	hum-ob mice	1309:1319	hum-ob mice	1309:1319	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	10	5	theme	gut	1551:1553	arg1	microbiota					1555:1564	the gut microbiota	1547:1564	the gut microbiota prior to nutritional intervention with prebiotics	1547:1614	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	4	6	theme	antibiotic-pretreated	597:617	arg1	mice					619:622	antibiotic-pretreated mice	597:622	antibiotic-pretreated mice (hum-ob mice; humanised obese mice)	597:658	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	0	7	theme	prebiotic	65:73	arg1	intervention					75:86	prebiotic intervention	65:86	prebiotic intervention	65:86	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	10	8	theme	obesity	1680:1686	arg1	context					1669:1675	the context	1665:1675	the context of obesity and metabolic disorders	1665:1710	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	8	9	theme	bacterial	1088:1096	arg1	genera					1098:1103	several bacterial genera	1080:1103	several bacterial genera	1080:1103	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	7	10	dep	RESULTS	902:908	arg1	show					913:916	show	913:916	show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet	913:1071	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	6	11	theme	obese	845:849	arg1	individuals					851:861	obese individuals	845:861	obese individuals supplemented with inulin for 3 months	845:899	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	0	12	theme	obese	91:95	arg1	patients					97:104	obese patients	91:104	obese patients	91:104	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	10	13	with	intervention	1587:1598	arg1	prebiotics					1605:1614	prebiotics	1605:1614	prebiotics	1605:1614	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	2	14	theme	related	275:281	arg1	diseases					283:290	related diseases	275:290	related diseases	275:290	Inulin as a prebiotic has been shown to lessen obesity and related diseases.					
32041744	7	15	theme	hum-ob	923:928	arg1	mice					930:933	hum-ob mice	923:933	hum-ob mice colonised with the faecal microbiota from different obese individuals	923:1003	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	10	16	dep	CONCLUSION	1493:1502	arg1	support					1519:1525	support	1519:1525	support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders	1519:1710	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	8	17	from	outcomes	1251:1258	arg1	mice					1316:1319	hum-ob mice	1309:1319	hum-ob mice	1309:1319	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	4	18	theme	obese	472:476	arg1	donors					478:483	four obese donors	467:483	four obese donors differing by microbial diversity and composition	467:532	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	4	19	theme	obese	648:652	arg1	mice					654:657	humanised obese mice	638:657	hum-ob mice; humanised obese mice	625:657	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	9	20	theme	index	1464:1468	arg1	decrease					1442:1449	the decrease	1438:1449	the decrease of body mass index in response to inulin	1438:1490	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	9	21	theme	body	1454:1457	arg1	index					1464:1468	body mass index	1454:1468	body mass index	1454:1468	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	10	22	theme	positive	1645:1652	arg1	outcome					1654:1660	the positive outcome	1641:1660	the positive outcome in the context of obesity and metabolic disorders	1641:1710	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	3	23	theme	physiological	410:422	arg1	response					424:431	the physiological response	406:431	the physiological response to inulin	406:441	The aim of the current study was to investigate whether preintervention gut microbiota characteristics determine the physiological response to inulin.					
32041744	9	24	theme	Akkermansia	1401:1411	arg1	levels					1377:1382	the preintervention levels	1357:1382	the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus	1357:1430	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	1	25	theme	interesting	160:170	arg1	target					184:189	an interesting therapeutic target	157:189	an interesting therapeutic target for metabolic disorders	157:213	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	1	25	theme	interesting	160:170	arg1	microbiota					125:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	3	26	theme	microbiota	369:378	arg1	characteristics					380:394	preintervention gut microbiota characteristics	349:394	preintervention gut microbiota characteristics	349:394	The aim of the current study was to investigate whether preintervention gut microbiota characteristics determine the physiological response to inulin.					
32041744	4	27	from	donors	478:483	arg1	stools					455:460	The stools	451:460	The stools from four obese donors differing by microbial diversity and composition	451:532	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	10	28	theme	nutritional	1575:1585	arg1	intervention					1587:1598	nutritional intervention	1575:1598	nutritional intervention with prebiotics	1575:1614	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	1	29	theme	therapeutic	172:182	arg1	target					184:189	an interesting therapeutic target	157:189	an interesting therapeutic target for metabolic disorders	157:213	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	1	29	theme	therapeutic	172:182	arg1	microbiota					125:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	9	30	theme	obese	1338:1342	arg1	individuals					1344:1354	obese individuals	1338:1354	obese individuals	1338:1354	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	8	31	theme	several	1080:1086	arg1	genera					1098:1103	several bacterial genera	1080:1103	several bacterial genera	1080:1103	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	6	32	theme	hum-ob	784:789	arg1	mice					791:794	hum-ob mice	784:794	hum-ob mice	784:794	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	3	33	theme	preintervention	349:363	arg1	characteristics					380:394	preintervention gut microbiota characteristics	349:394	preintervention gut microbiota characteristics	349:394	The aim of the current study was to investigate whether preintervention gut microbiota characteristics determine the physiological response to inulin.					
32041744	4	34	theme	dietary	558:564	arg1	intervention					566:577	the dietary intervention	554:577	the dietary intervention	554:577	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	8	35	dep	outcomes	1251:1258	arg1	decrease					1261:1268	decrease	1261:1268	decrease in adiposity and hepatic steatosis	1261:1303	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	7	36	theme	faecal	954:959	arg1	microbiota					961:970	the faecal microbiota	950:970	the faecal microbiota	950:970	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	8	37	theme	observed	1232:1239	arg1	outcomes					1251:1258	the observed metabolic outcomes	1228:1258	the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice	1228:1319	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	8	38	theme	hepatic	1287:1293	arg1	steatosis					1295:1303	hepatic steatosis	1287:1303	hepatic steatosis	1287:1303	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	3	39	theme	gut	365:367	arg1	characteristics					380:394	preintervention gut microbiota characteristics	349:394	preintervention gut microbiota characteristics	349:394	The aim of the current study was to investigate whether preintervention gut microbiota characteristics determine the physiological response to inulin.					
32041744	1	40	theme	metabolic	195:203	arg1	disorders					205:213	metabolic disorders	195:213	metabolic disorders	195:213	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	0	41	theme	signature	31:39	arg1	Discovery					0:8	Discovery	0:8	Discovery of the gut microbial signature	0:39	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	6	42	from	changes	753:759	arg1	mice					791:794	hum-ob mice	784:794	hum-ob mice	784:794	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	6	42	from	changes	753:759	arg1	treatment					771:779	inulin treatment	764:779	inulin treatment	764:779	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	4	43	dep	mice	619:622	arg1	mice					632:635	hum-ob mice	625:635	hum-ob mice; humanised obese mice	625:657	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	10	44	from	outcome	1654:1660	arg1	context					1669:1675	the context	1665:1675	the context of obesity and metabolic disorders	1665:1710	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	10	45	theme	disorders	1702:1710	arg1	context					1669:1675	the context	1665:1675	the context of obesity and metabolic disorders	1665:1710	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	0	46	theme	microbial	21:29	arg1	signature					31:39	the gut microbial signature	13:39	the gut microbial signature	13:39	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	9	47	theme	preintervention	1361:1375	arg1	levels					1377:1382	the preintervention levels	1357:1382	the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus	1357:1430	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	6	48	theme	individuals	851:861	arg1	cohort					835:840	a cohort	833:840	a cohort of obese individuals supplemented with inulin for 3 months	833:899	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	0	49	theme	gut	17:19	arg1	signature					31:39	the gut microbial signature	13:39	the gut microbial signature	13:39	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	7	50	theme	inulin	1031:1036	arg1	supplementation					1038:1052	inulin supplementation	1031:1052	inulin supplementation	1031:1052	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	10	51	theme	metabolic	1692:1700	arg1	disorders					1702:1710	metabolic disorders	1692:1710	metabolic disorders	1692:1710	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	7	52	theme	high-fat	1059:1066	arg1	diet					1068:1071	a high-fat diet	1057:1071	a high-fat diet	1057:1071	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	0	53	from	efficacy	53:60	arg1	patients					97:104	obese patients	91:104	obese patients	91:104	Discovery of the gut microbial signature driving the efficacy of prebiotic intervention in obese patients.					
32041744	6	54	theme	inulin	764:769	arg1	treatment					771:779	inulin treatment	764:779	inulin treatment	764:779	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	1	55	theme	OBJECTIVE	107:115	arg1	microbiota					125:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	1	55	theme	OBJECTIVE	107:115	arg1	target					184:189	an interesting therapeutic target	157:189	an interesting therapeutic target for metabolic disorders	157:213	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	8	56	theme	metabolic	1241:1249	arg1	outcomes					1251:1258	the observed metabolic outcomes	1228:1258	the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice	1228:1319	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	9	57	from	decrease	1442:1449	arg1	response					1473:1480	response	1473:1480	response to inulin	1473:1490	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	10	58	theme	prior	1566:1570	arg1	microbiota					1555:1564	the gut microbiota	1547:1564	the gut microbiota prior to nutritional intervention with prebiotics	1547:1614	CONCLUSION These findings support that characterising the gut microbiota prior to nutritional intervention with prebiotics is important to increase the positive outcome in the context of obesity and metabolic disorders.					
32041744	8	59	from	decrease	1261:1268	arg1	adiposity					1273:1281	adiposity	1273:1281	adiposity	1273:1281	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	8	59	from	decrease	1261:1268	arg1	steatosis					1295:1303	hepatic steatosis	1287:1303	hepatic steatosis	1287:1303	Among several bacterial genera, Barnesiella, Bilophila, Butyricimonas, Victivallis, Clostridium XIVa, Akkermansia, Raoultella and Blautia correlated with the observed metabolic outcomes (decrease in adiposity and hepatic steatosis) in hum-ob mice.					
32041744	1	60	theme	The	117:119	arg1	microbiota					125:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	1	60	theme	The	117:119	arg1	target					184:189	an interesting therapeutic target	157:189	an interesting therapeutic target for metabolic disorders	157:213	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	9	61	theme	Anaerostipes	1387:1398	arg1	levels					1377:1382	the preintervention levels	1357:1382	the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus	1357:1430	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	5	62	theme	high-fat	689:696	arg1	diet					698:701	a high-fat diet	687:701	a high-fat diet	687:701	Hum-ob mice were fed with a high-fat diet and treated with inulin.					
32041744	1	63	theme	gut	121:123	arg1	microbiota					125:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota	107:134	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	1	63	theme	gut	121:123	arg1	target					184:189	an interesting therapeutic target	157:189	an interesting therapeutic target for metabolic disorders	157:213	OBJECTIVE The gut microbiota has been proposed as an interesting therapeutic target for metabolic disorders.					
32041744	7	64	theme	different	977:985	arg1	individuals					993:1003	different obese individuals	977:1003	different obese individuals	977:1003	RESULTS We show that hum-ob mice colonised with the faecal microbiota from different obese individuals differentially respond to inulin supplementation on a high-fat diet.					
32041744	4	65	dep	DESIGN	444:449	arg1	sampled					539:545	sampled	539:545	sampled before the dietary intervention	539:577	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	4	65	dep	DESIGN	444:449	arg1	inoculated					583:592	inoculated	583:592	inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice)	583:658	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	4	66	dep	mice	632:635	arg1	mice					654:657	humanised obese mice	638:657	hum-ob mice; humanised obese mice	625:657	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	6	67	theme	microbiota	742:751	arg1	changes					753:759	Metabolic and microbiota changes	728:759	changes	753:759	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	4	68	theme	hum-ob	625:630	arg1	mice					632:635	hum-ob mice	625:635	hum-ob mice; humanised obese mice	625:657	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	3	69	theme	current	308:314	arg1	study					316:320	the current study	304:320	the current study	304:320	The aim of the current study was to investigate whether preintervention gut microbiota characteristics determine the physiological response to inulin.					
32041744	9	70	theme	mass	1459:1462	arg1	index					1464:1468	body mass index	1454:1468	body mass index	1454:1468	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
32041744	4	71	theme	humanised	638:646	arg1	mice					654:657	humanised obese mice	638:657	hum-ob mice; humanised obese mice	625:657	DESIGN The stools from four obese donors differing by microbial diversity and composition were sampled before the dietary intervention and inoculated to antibiotic-pretreated mice (hum-ob mice; humanised obese mice).					
32041744	3	72	theme	study	316:320	arg1	aim					297:299	The aim	293:299	The aim of the current study	293:320	The aim of the current study was to investigate whether preintervention gut microbiota characteristics determine the physiological response to inulin.					
32041744	6	73	theme	Metabolic	728:736	arg1	changes					753:759	Metabolic and microbiota changes	728:759	changes	753:759	Metabolic and microbiota changes on inulin treatment in hum-ob mice were compared with those obtained in a cohort of obese individuals supplemented with inulin for 3 months.					
32041744	9	74	theme	Butyricicoccus	1417:1430	arg1	levels					1377:1382	the preintervention levels	1357:1382	the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus	1357:1430	In addition, in obese individuals, the preintervention levels of Anaerostipes, Akkermansia and Butyricicoccus drive the decrease of body mass index in response to inulin.					
34126154	0	0	theme	versatile	80:88	arg1	catalyst					90:97	A versatile catalyst	78:97	A versatile catalyst for p-nitrophenol reduction	78:125	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	4	1	from	effective	995:1003	arg1	reaction					1036:1043	the Suzuki-Miyaura coupling reaction	1008:1043	the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance	1008:1097	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	2	2	theme	unnecessary	798:808	arg1	leaching					810:817	unnecessary leaching	798:817	unnecessary leaching of nanoparticles	798:834	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	5	3	theme	recovery	1219:1226	arg1	efficiency					1228:1237	high recovery efficiency	1214:1237	high recovery efficiency	1214:1237	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	5	4	contain	have	1209:1212	arg1	microcapsules					1195:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	5	4	contain	have	1209:1212	arg2	efficiency					1228:1237	high recovery efficiency	1214:1237	high recovery efficiency	1214:1237	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	6	5	theme	heterogeneous	1365:1377	arg1	nature					1379:1384	heterogeneous nature	1365:1384	heterogeneous nature	1365:1384	The hot filtration test indicated that the catalyst has heterogeneous nature.					
34126154	5	6	theme	Fe3O4/CS-Me	1180:1190	arg1	microcapsules					1195:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	3	7	theme	Fe3O4/CS-Me	846:856	arg1	microcapsules					861:873	Fe3O4/CS-Me@Pd microcapsules	846:873	Fe3O4/CS-Me@Pd microcapsules	846:873	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	5	8	theme	high	1214:1217	arg1	efficiency					1228:1237	high recovery efficiency	1214:1237	high recovery efficiency	1214:1237	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	1	9	theme	X-ray	445:449	arg1	XRD					464:466	XRD	464:466	XRD	464:466	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	9	theme	X-ray	445:449	arg1	diffraction					451:461	X-ray diffraction	445:461	X-ray diffraction (XRD)	445:467	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	3	10	theme	high	885:888	arg1	performance					900:910	high catalytic performance	885:910	high catalytic performance in reducing p-NP in water at room temperature (150-300 s)	885:968	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	2	11	theme	palladium	734:742	arg1	distribution					718:729	the uniform distribution	706:729	the uniform distribution of palladium (II)	706:747	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	1	12	theme	infrared	378:385	arg1	FT-IR					401:405	FT-IR	401:405	FT-IR	401:405	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	12	theme	infrared	378:385	arg1	spectroscopy					387:398	infrared spectroscopy	378:398	infrared spectroscopy (FT-IR)	378:406	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	3	13	theme	Pd	858:859	arg1	microcapsules					861:873	Fe3O4/CS-Me@Pd microcapsules	846:873	Fe3O4/CS-Me@Pd microcapsules	846:873	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	1	14	theme	Energy-dispersive	526:542	arg1	EDX					564:566	EDX	564:566	EDX	564:566	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	14	theme	Energy-dispersive	526:542	arg1	spectroscopy					550:561	Energy-dispersive X-ray spectroscopy	526:561	Energy-dispersive X-ray spectroscopy (EDX)	526:567	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	0	15	theme	p-nitrophenol	103:115	arg1	reduction					117:125	p-nitrophenol reduction	103:125	p-nitrophenol reduction	103:125	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	2	16	theme	coordination	616:627	arg1	point					629:633	a coordination point	614:633	a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles	614:834	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	1	17	theme	X-ray	544:548	arg1	EDX					564:566	EDX	564:566	EDX	564:566	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	17	theme	X-ray	544:548	arg1	spectroscopy					550:561	Energy-dispersive X-ray spectroscopy	526:561	Energy-dispersive X-ray spectroscopy (EDX)	526:567	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	5	18	theme	Pd	1192:1193	arg1	microcapsules					1195:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	4	19	dep	effective	995:1003	arg1	TON = 3.8 × 104					1100:1114	TON = 3.8 × 104	1100:1114	TON = 3.8 × 104	1100:1114	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	1	20	with	catalyst	217:224	arg1	recoverability					258:271	recoverability	258:271	recoverability	258:271	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	20	with	catalyst	217:224	arg1	stability					301:309	excellent stability	291:309	excellent stability	291:309	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	20	with	catalyst	217:224	arg1	efficiency					236:245	high efficiency	231:245	high efficiency	231:245	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	20	with	catalyst	217:224	arg1	magnetic					248:255	magnetic	248:255	magnetic	248:255	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	20	with	catalyst	217:224	arg1	reusability					274:284	reusability	274:284	reusability	274:284	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	4	21	with	reaction	1036:1043	arg1	performance					1087:1097	high catalytic performance	1072:1097	high catalytic performance	1072:1097	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	2	22	theme	microspheres	662:673	arg1	surface					642:648	the surface	638:648	the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles	638:834	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	6	23	contain	has	1361:1363	arg1	catalyst					1352:1359	the catalyst	1348:1359	the catalyst	1348:1359	The hot filtration test indicated that the catalyst has heterogeneous nature.					
34126154	6	23	contain	has	1361:1363	arg2	nature					1379:1384	heterogeneous nature	1365:1384	heterogeneous nature	1365:1384	The hot filtration test indicated that the catalyst has heterogeneous nature.					
34126154	1	24	dep	Fourier	360:366	arg1	transform					368:376	transform	368:376	transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX)	368:567	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	0	25	theme	Suzuki	131:136	arg1	reaction					138:145	Suzuki reaction	131:145	Suzuki reaction in aqueous medium	131:163	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	2	26	theme	chitosan	653:660	arg1	microspheres					662:673	chitosan microspheres	653:673	chitosan microspheres	653:673	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	4	27	theme	catalytic	1077:1085	arg1	performance					1087:1097	high catalytic performance	1072:1097	high catalytic performance	1072:1097	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	1	28	theme	environmental-friendly	184:205	arg1	catalyst					217:224	an environmental-friendly palladium catalyst	181:224	an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability	181:309	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	29	theme	excellent	291:299	arg1	stability					301:309	excellent stability	291:309	excellent stability	291:309	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	5	30	theme	@	1191:1191	arg1	microcapsules					1195:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Fe3O4/CS-Me@Pd microcapsules	1180:1207	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	1	31	theme	palladium	207:215	arg1	catalyst					217:224	an environmental-friendly palladium catalyst	181:224	an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability	181:309	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	0	32	theme	functionalized	38:51	arg1	beads					71:75	melamine functionalized magnetic chitosan beads	29:75	melamine functionalized magnetic chitosan beads	29:75	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	3	33	theme	catalytic	890:898	arg1	performance					900:910	high catalytic performance	885:910	high catalytic performance in reducing p-NP in water at room temperature (150-300 s)	885:968	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	0	34	theme	melamine	29:36	arg1	beads					71:75	melamine functionalized magnetic chitosan beads	29:75	melamine functionalized magnetic chitosan beads	29:75	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	5	35	theme	Reproducibility	1135:1149	arg1	experiments					1151:1161	Reproducibility experiments	1135:1161	Reproducibility experiments	1135:1161	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	6	36	theme	filtration	1317:1326	arg1	test					1328:1331	The hot filtration test	1309:1331	The hot filtration test	1309:1331	The hot filtration test indicated that the catalyst has heterogeneous nature.					
34126154	1	37	theme	Elemental	470:478	arg1	mapping					480:486	Elemental mapping	470:486	Elemental mapping	470:486	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	0	38	theme	chitosan	62:69	arg1	beads					71:75	melamine functionalized magnetic chitosan beads	29:75	melamine functionalized magnetic chitosan beads	29:75	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	6	39	theme	hot	1313:1315	arg1	test					1328:1331	The hot filtration test	1309:1331	The hot filtration test	1309:1331	The hot filtration test indicated that the catalyst has heterogeneous nature.					
34126154	1	40	theme	Scanning	409:416	arg1	microscopy					427:436	Scanning electron microscopy	409:436	Scanning electron microscopy (SEM)	409:442	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	40	theme	Scanning	409:416	arg1	SEM					439:441	SEM	439:441	SEM	439:441	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	3	41	theme	room	941:944	arg1	150-300 s					959:967	150-300 s	959:967	150-300 s	959:967	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	3	41	theme	room	941:944	arg1	temperature					946:956	room temperature	941:956	room temperature (150-300 s)	941:968	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	0	42	theme	magnetic	53:60	arg1	beads					71:75	melamine functionalized magnetic chitosan beads	29:75	melamine functionalized magnetic chitosan beads	29:75	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	2	43	from	point	629:633	arg1	surface					642:648	the surface	638:648	the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles	638:834	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	0	44	theme	aqueous	150:156	arg1	medium					158:163	aqueous medium	150:163	aqueous medium	150:163	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	1	45	theme	electron	418:425	arg1	microscopy					427:436	Scanning electron microscopy	409:436	Scanning electron microscopy (SEM)	409:442	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	45	theme	electron	418:425	arg1	SEM					439:441	SEM	439:441	SEM	439:441	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	3	46	from	p-NP	924:927	arg1	water					932:936	water	932:936	water	932:936	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
34126154	1	47	theme	Thermogravimetric	489:505	arg1	TGA					517:519	TGA	517:519	TGA	517:519	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	1	47	theme	Thermogravimetric	489:505	arg1	analysis					507:514	Thermogravimetric analysis	489:514	Thermogravimetric analysis (TGA)	489:520	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	4	48	theme	mild	1051:1054	arg1	conditions					1056:1065	mild conditions	1051:1065	mild conditions	1051:1065	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	1	49	theme	high	231:234	arg1	efficiency					236:245	high efficiency	231:245	high efficiency	231:245	In this study, an environmental-friendly palladium catalyst with high efficiency, magnetic, recoverability, reusability, and excellent stability was prepared and thoroughly characterized by the Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD), Elemental mapping, Thermogravimetric analysis (TGA) and Energy-dispersive X-ray spectroscopy (EDX).					
34126154	4	50	theme	Suzuki-Miyaura	1012:1025	arg1	reaction					1036:1043	the Suzuki-Miyaura coupling reaction	1008:1043	the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance	1008:1097	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	4	51	dep	TON = 3.8 × 104	1100:1114	arg1	TOF = 7.6 × 104					1117:1131	TOF = 7.6 × 104	1117:1131	TOF = 7.6 × 104	1117:1131	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	4	52	theme	high	1072:1075	arg1	performance					1087:1097	high catalytic performance	1072:1097	high catalytic performance	1072:1097	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	0	53	from	reaction	138:145	arg1	medium					158:163	aqueous medium	150:163	aqueous medium	150:163	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	0	54	from	catalyst	90:97	arg1	medium					158:163	aqueous medium	150:163	aqueous medium	150:163	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	4	55	theme	coupling	1027:1034	arg1	reaction					1036:1043	the Suzuki-Miyaura coupling reaction	1008:1043	the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance	1008:1097	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	5	56	theme	catalytic	1288:1296	arg1	reactions					1298:1306	these two catalytic reactions	1278:1306	these two catalytic reactions	1278:1306	Reproducibility experiments also showed that Fe3O4/CS-Me@Pd microcapsules have high recovery efficiency and can work at least six times during these two catalytic reactions.					
34126154	2	57	theme	nanoparticles	822:834	arg1	leaching					810:817	unnecessary leaching	798:817	unnecessary leaching of nanoparticles	798:834	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	0	58	dep	Immobilizing	0:11	arg1	reaction					138:145	Suzuki reaction	131:145	Suzuki reaction in aqueous medium	131:163	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	0	58	dep	Immobilizing	0:11	arg1	catalyst					90:97	A versatile catalyst	78:97	A versatile catalyst for p-nitrophenol reduction	78:125	Immobilizing of palladium on melamine functionalized magnetic chitosan beads: A versatile catalyst for p-nitrophenol reduction and Suzuki reaction in aqueous medium.					
34126154	2	59	theme	uniform	710:716	arg1	distribution					718:729	the uniform distribution	706:729	the uniform distribution of palladium (II)	706:747	Results demonstrates that melamine provides a coordination point on the surface of chitosan microspheres, which provides a platform for the uniform distribution of palladium (II) and combines with palladium (II) firmly to avoid unnecessary leaching of nanoparticles.					
34126154	4	60	from	reaction	1036:1043	arg1	effective					995:1003	effective	995:1003	effective	995:1003	This composite was also effective in the Suzuki-Miyaura coupling reaction under mild conditions with high catalytic performance (TON = 3.8 × 104, TOF = 7.6 × 104).					
34126154	3	61	theme	@	857:857	arg1	microcapsules					861:873	Fe3O4/CS-Me@Pd microcapsules	846:873	Fe3O4/CS-Me@Pd microcapsules	846:873	Besides, Fe3O4/CS-Me@Pd microcapsules exhibited high catalytic performance in reducing p-NP in water at room temperature (150-300 s).					
32342818	5	0	dep	%	1518:1518	arg1	glucose					1772:1778	D glucose	1770:1778	D glucose	1770:1778	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	0	dep	%	1518:1518	arg1	-β					1765:1766	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	carboxymethyl-					1691:1704	carboxymethyl-	1691:1704	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	β					1716:1716	β	1716:1716	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	hydroxyl-poly					1668:1680	diethyl hydroxyl-poly -2	1660:1683	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	-dicarboxymethyl-					1739:1755	O -dicarboxymethyl-	1737:1755	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	→					1709:1709	1 → 4	1707:1711	1 → 4	1707:1711	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	glucose					1722:1728	D glucose -2.6	1720:1733	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	glucose					1650:1656	ox glucose	1647:1656	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	1	dep	D	1643:1643	arg1	O					1687:1687	O	1687:1687	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	2	theme	Na-	2318:2320	arg1	CMC					2322:2324	Na- CMC	2318:2324	Na- CMC	2318:2324	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	3	theme	carboxymethyl-	1559:1572	arg1	→					1577:1577	1 → 4	1575:1579	1 → 4	1575:1579	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	3	theme	carboxymethyl-	1559:1572	arg1	-β					1582:1583	carboxymethyl- (1 → 4) -β	1559:1583	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	6	4	theme	films	1961:1965	arg1	scission					1896:1903	the bio scission time and physicochemical parameters	1888:1939	scission	1896:1903	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	6	4	theme	films	1961:1965	arg1	parameters					1930:1939	physicochemical parameters	1914:1939	physicochemical parameters	1914:1939	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	7	5	theme	composition	2303:2313	arg1	substance					2341:2349	a "CelAgrip" substance	2328:2349	a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm	2328:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	5	theme	composition	2303:2313	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	5	theme	composition	2303:2313	arg1	value					2426:2430	a pH value	2421:2430	a pH value of 7.6	2421:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	6	dep	METHODS	988:994	arg1	based					1094:1098	based	1094:1098	is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1091:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	7	theme	2,3-diethoxy	1536:1547	arg1	-β					1638:1639	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	8	theme	O	1737:1737	arg1	-dicarboxymethyl-					1739:1755	O -dicarboxymethyl-	1737:1755	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	9	theme	diethyl	1660:1666	arg1	hydroxyl-poly					1668:1680	diethyl hydroxyl-poly -2	1660:1683	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	6	10	theme	ocular	1944:1949	arg1	films					1961:1965	ocular medicinal films	1944:1965	ocular medicinal films	1944:1965	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	2	11	theme	ophthalmologists	269:284	arg1	majority					257:264	The vast majority	248:264	The vast majority of ophthalmologists	248:284	The vast majority of ophthalmologists use traditional instillation of drugs in an eye disease.					
32342818	7	12	theme	7.6	2435:2437	arg1	substance					2341:2349	a "CelAgrip" substance	2328:2349	a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm	2328:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	12	theme	7.6	2435:2437	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	12	theme	7.6	2435:2437	arg1	value					2426:2430	a pH value	2421:2430	a pH value of 7.6	2421:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	13	theme	±	1389:1389	arg1	±					1389:1389	polymerization-630 ± 20	1370:1392	polymerization-630 ± 20	1370:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	13	theme	±	1389:1389	arg1	amount					1360:1365	an amount	1357:1365	an amount of polymerization-630 ± 20	1357:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	3	14	theme	fluid	510:514	arg1	withdrawal					487:496	withdrawal	487:496	withdrawal of its tear fluid and rapid absorption	487:535	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	6	15	theme	substitution	2008:2019	arg1	degree					1998:2003	the degree	1994:2003	the degree of substitution	1994:2019	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	6	15	theme	substitution	2008:2019	arg1	degree					2025:2030	degree	2025:2030	degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions	2025:2119	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	5	16	theme	obtaining	1008:1016	arg1	forms					1028:1032	obtaining polymeric forms	1008:1032	obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films	1008:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	17	theme	therapeutic	879:889	arg1	concentration					891:903	its therapeutic concentration	875:903	its therapeutic concentration	875:903	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	18	theme	forms	1028:1032	arg1	method					998:1003	A method	996:1003	A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films	996:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	1	19	theme	diseases	216:223	arg1	treatment					203:211	treatment	203:211	treatment	203:211	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	1	19	theme	diseases	216:223	arg1	prevention					188:197	prevention	188:197	prevention	188:197	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	7	20	with	shape	2366:2370	arg1	nanoparticles					2386:2398	embedded nanoparticles	2377:2398	embedded nanoparticles	2377:2398	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	3	21	theme	rapid	520:524	arg1	absorption					526:535	rapid absorption	520:535	rapid absorption	520:535	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	6	22	theme	pH	2091:2092	arg1	polymerization					2035:2048	polymerization	2035:2048	polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions	2035:2119	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	7	23	theme	pH	2423:2424	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	23	theme	pH	2423:2424	arg1	value					2426:2430	a pH value	2421:2430	a pH value of 7.6	2421:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	24	theme	nm	2414:2415	arg1	shape					2366:2370	a spherical shape	2354:2370	a spherical shape with embedded nanoparticles of size 14-52 nm	2354:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	25	theme	drug	1821:1824	arg1	"					1835:1835	the antiviral drug "CelAgrip"	1807:1835	the antiviral drug "CelAgrip"	1807:1835	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	26	theme	size	2403:2406	arg1	nm					2414:2415	size 14-52 nm	2403:2415	size 14-52 nm	2403:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	27	theme	CelAgrip	1827:1834	arg1	"					1835:1835	the antiviral drug "CelAgrip"	1807:1835	the antiviral drug "CelAgrip"	1807:1835	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	0	28	theme	Anti-	63:67	arg1	Agents					75:80	Anti- Viral Agents	63:80	Anti- Viral Agents	63:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	5	29	theme	medicinal	1075:1083	arg1	films					1085:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	3	30	theme	mucous	544:549	arg1	membrane					551:558	the mucous membrane	540:558	the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball	540:675	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	6	31	theme	sodium	2053:2058	arg1	carboxymethylcellulose					2060:2081	sodium carboxymethylcellulose	2053:2081	sodium carboxymethylcellulose	2053:2081	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	6	32	theme	medicinal	1951:1959	arg1	films					1961:1965	ocular medicinal films	1944:1965	ocular medicinal films	1944:1965	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	4	33	theme	drug	933:936	arg1	effect					955:960	the negative effect	942:960	the negative effect of frequent instillation	942:985	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	4	33	theme	drug	933:936	arg1	dosage					919:924	the dosage	915:924	the dosage of the drug	915:936	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	34	theme	natural	1468:1474	arg1	polyphenol-gossypol					1476:1494	chemically bound natural polyphenol-gossypol	1451:1494	chemically bound natural polyphenol-gossypol	1451:1494	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	0	35	theme	Agents	75:80	arg1	Administration					45:58	Ophthalmic Administration	34:58	Ophthalmic Administration of Anti- Viral Agents	34:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	1	36	theme	eye	169:171	arg1	tissue					173:178	eye tissue	169:178	eye tissue for the prevention and treatment of diseases of various etiologies	169:245	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	6	37	theme	polymerization	2035:2048	arg1	degree					1998:2003	the degree	1994:2003	the degree of substitution	1994:2019	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	6	37	theme	polymerization	2035:2048	arg1	degree					2025:2030	degree	2025:2030	degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions	2025:2119	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	4	38	theme	polymeric	699:707	arg1	forms					709:713	polymeric forms	699:713	polymeric forms of bio soluble antiviral eye medicinal films of prolonged form	699:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	39	contain	containing	1440:1449	arg1	Na-carboxymethylcellulose					1414:1438	Na-carboxymethylcellulose	1414:1438	Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1414:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	39	contain	containing	1440:1449	arg2	polyphenol-gossypol					1476:1494	chemically bound natural polyphenol-gossypol	1451:1494	chemically bound natural polyphenol-gossypol	1451:1494	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	6	40	dep	work	1853:1856	arg1	shown					1976:1980	shown	1976:1980	shown	1976:1980	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	5	41	theme	bio	1037:1039	arg1	films					1085:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	2	42	theme	traditional	290:300	arg1	instillation					302:313	traditional instillation	290:313	traditional instillation of drugs	290:322	The vast majority of ophthalmologists use traditional instillation of drugs in an eye disease.					
32342818	5	43	theme	Na-carboxymethylcellulose	1414:1438	arg1	derivative					1400:1409	a derivative	1398:1409	a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1398:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	43	theme	Na-carboxymethylcellulose	1414:1438	arg1	polyphenol-gossypol					1334:1352	a chemically bound natural polyphenol-gossypol	1307:1352	a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20	1307:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	44	theme	spherical	2356:2364	arg1	shape					2366:2370	a spherical shape	2354:2370	a spherical shape with embedded nanoparticles of size 14-52 nm	2354:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	45	theme	nanostructured	1049:1062	arg1	films					1085:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	46	from	use	692:694	arg1	practice					792:799	ophthalmic practice	781:799	ophthalmic practice	781:799	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	4	47	theme	frequent	965:972	arg1	instillation					974:985	frequent instillation	965:985	frequent instillation	965:985	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	0	48	theme	Drug	0:3	arg1	Systems					22:28	Drug Delivery Polymer Systems	0:28	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents	0:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	5	49	theme	water-soluble	1120:1132	arg1	Na-carboxymethylcellulose					1134:1158	water-soluble Na-carboxymethylcellulose	1120:1158	water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1120:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	49	theme	water-soluble	1120:1132	arg1	Na-CMC					1161:1166	Na-CMC	1161:1166	Na-CMC	1161:1166	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	6	50	theme	solutions	2111:2119	arg1	carboxymethylcellulose					2060:2081	sodium carboxymethylcellulose	2053:2081	sodium carboxymethylcellulose	2053:2081	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	6	50	theme	solutions	2111:2119	arg1	pH					2091:2092	the pH	2087:2092	the pH of their aqueous solutions	2087:2119	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	7	51	theme	transparency	2232:2243	arg1	CONCLUSION					2122:2131	CONCLUSION	2122:2131	CONCLUSION The most promising in terms of the prolongation effect	2122:2186	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	51	theme	transparency	2232:2243	arg1	absence					2196:2202	the absence	2192:2202	the absence of an irritating effect and transparency	2192:2243	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	51	theme	transparency	2232:2243	arg1	films					2249:2253	films	2249:2253	films	2249:2253	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	52	theme	%	1518:1518	arg1	amount					1502:1507	an amount	1499:1507	an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1499:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	52	theme	%	1518:1518	arg1	%					1518:1518	0 mole%	1512:1518	0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1512:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	53	theme	"	2339:2339	arg1	substance					2341:2349	a "CelAgrip" substance	2328:2349	a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm	2328:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	53	theme	"	2339:2339	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	3	54	theme	drugs	443:447	arg1	forms					434:438	drip forms	429:438	drip forms of drugs	429:447	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	0	55	theme	Polymer	14:20	arg1	Systems					22:28	Drug Delivery Polymer Systems	0:28	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents	0:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	6	56	theme	physicochemical	1914:1928	arg1	parameters					1930:1939	physicochemical parameters	1914:1939	physicochemical parameters	1914:1939	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	1	57	theme	many	116:119	arg1	ways					131:134	many different ways	116:134	many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies	116:245	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	7	58	theme	effect	2221:2226	arg1	CONCLUSION					2122:2131	CONCLUSION	2122:2131	CONCLUSION The most promising in terms of the prolongation effect	2122:2186	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	58	theme	effect	2221:2226	arg1	absence					2196:2202	the absence	2192:2202	the absence of an irritating effect and transparency	2192:2243	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	58	theme	effect	2221:2226	arg1	films					2249:2253	films	2249:2253	films	2249:2253	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	59	theme	sodium	1521:1526	arg1	salt					1528:1531	sodium salt	1521:1531	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	60	theme	films	754:758	arg1	forms					709:713	polymeric forms	699:713	polymeric forms of bio soluble antiviral eye medicinal films of prolonged form	699:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	3	61	theme	forms	434:438	arg1	instillation					413:424	instillation	413:424	instillation of drip forms of drugs	413:447	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	3	62	theme	eyes	567:570	arg1	membrane					551:558	the mucous membrane	540:558	the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball	540:675	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	4	63	theme	prolonged	763:771	arg1	form					773:776	prolonged form	763:776	prolonged form	763:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	6	64	theme	bio	1892:1894	arg1	scission					1896:1903	the bio scission time and physicochemical parameters	1888:1939	scission	1896:1903	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	3	65	contain	has	364:366	arg1	method					357:362	this method	352:362	this method	352:362	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	3	65	contain	has	364:366	arg2	number					370:375	a number	368:375	a number of drawbacks	368:388	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	4	66	theme	soluble	722:728	arg1	films					754:758	bio soluble antiviral eye medicinal films	718:758	bio soluble antiviral eye medicinal films of prolonged form	718:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	4	67	theme	medicinal	744:752	arg1	films					754:758	bio soluble antiviral eye medicinal films	718:758	bio soluble antiviral eye medicinal films of prolonged form	718:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	68	theme	-β	1638:1639	arg1	salt					1528:1531	sodium salt	1521:1531	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	→					1577:1577	1 → 4	1575:1579	1 → 4	1575:1579	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	glucose					1594:1600	ox glucose	1591:1600	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	O					1555:1555	O	1555:1555	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	ethyl-					1623:1628	ethyl-	1623:1628	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	-β					1582:1583	carboxymethyl- (1 → 4) -β	1559:1583	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	→					1633:1633	1 → 4	1631:1635	1 → 4	1631:1635	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	oxy-hydroxyl					1604:1615	oxy-hydroxyl	1604:1615	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	D					1587:1587	D	1587:1587	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	6					1551:1551	6	1551:1551	6	1551:1551	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	69	dep	2,3-diethoxy	1536:1547	arg1	2					1619:1619	2	1619:1619	2	1619:1619	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	70	theme	CMC	2322:2324	arg1	composition					2303:2313	the polymer-polymer composition	2283:2313	the polymer-polymer composition of Na- CMC	2283:2324	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	4	71	theme	eye	740:742	arg1	films					754:758	bio soluble antiviral eye medicinal films	718:758	bio soluble antiviral eye medicinal films of prolonged form	718:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	3	72	theme	drug	467:470	arg1	%					458:458	up to 80%	450:458	up to 80% of the drug	450:470	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	3	72	theme	drug	467:470	arg1	drug					467:470	the drug	463:470	the drug	463:470	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	1	73	theme	etiologies	236:245	arg1	diseases					216:223	diseases	216:223	diseases of various etiologies	216:245	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	7	74	theme	prolongation	2168:2179	arg1	effect					2181:2186	the prolongation effect	2164:2186	the prolongation effect	2164:2186	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	3	75	theme	therapeutic	636:646	arg1	concentration					648:660	the therapeutic concentration	632:660	the therapeutic concentration in the eyeball	632:675	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	5	76	theme	natural	1326:1332	arg1	polyphenol-gossypol					1334:1352	a chemically bound natural polyphenol-gossypol	1307:1352	a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20	1307:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	77	theme	polymer-polymer	2287:2301	arg1	composition					2303:2313	the polymer-polymer composition	2283:2313	the polymer-polymer composition of Na- CMC	2283:2324	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	78	contain	containing	1296:1305	arg2	derivative					1400:1409	a derivative	1398:1409	a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1398:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	78	contain	containing	1296:1305	arg1	Nacarboxymethylcellulose					1271:1294	Nacarboxymethylcellulose	1271:1294	Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1271:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	78	contain	containing	1296:1305	arg2	polyphenol-gossypol					1334:1352	a chemically bound natural polyphenol-gossypol	1307:1352	a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20	1307:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	79	theme	D	1643:1643	arg1	salt					1528:1531	sodium salt	1521:1531	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	80	theme	form	773:776	arg1	films					754:758	bio soluble antiviral eye medicinal films	718:758	bio soluble antiviral eye medicinal films of prolonged form	718:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	3	81	from	concentration	648:660	arg1	eyeball					669:675	the eyeball	665:675	the eyeball	665:675	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	3	82	theme	tear	505:508	arg1	fluid					510:514	its tear fluid	501:514	its tear fluid	501:514	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	4	83	theme	ophthalmic	781:790	arg1	practice					792:799	ophthalmic practice	781:799	ophthalmic practice	781:799	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	7	84	from	promising	2142:2150	arg1	terms					2155:2159	terms	2155:2159	terms of the prolongation effect	2155:2186	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	85	with	Na-carboxymethylcellulose	1134:1158	arg1	degree					1232:1237	a polymerization degree	1215:1237	a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1215:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	85	with	Na-carboxymethylcellulose	1134:1158	arg1	degree					1176:1181	a degree	1174:1181	a degree of substitution of 0.85 ± 2	1174:1209	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	2	86	theme	drugs	318:322	arg1	instillation					302:313	traditional instillation	290:313	traditional instillation of drugs	290:322	The vast majority of ophthalmologists use traditional instillation of drugs in an eye disease.					
32342818	7	87	theme	promising	2142:2150	arg1	CONCLUSION					2122:2131	CONCLUSION	2122:2131	CONCLUSION The most promising in terms of the prolongation effect	2122:2186	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	87	theme	promising	2142:2150	arg1	films					2249:2253	films	2249:2253	films	2249:2253	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	87	theme	promising	2142:2150	arg1	absence					2196:2202	the absence	2192:2202	the absence of an irritating effect and transparency	2192:2243	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	88	from	terms	2155:2159	arg1	promising					2142:2150	promising	2142:2150	promising	2142:2150	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	89	theme	polymeric	1018:1026	arg1	forms					1028:1032	obtaining polymeric forms	1008:1032	obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films	1008:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	3	90	theme	absorption	526:535	arg1	withdrawal					487:496	withdrawal	487:496	withdrawal of its tear fluid and rapid absorption	487:535	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	5	91	theme	D	1770:1770	arg1	glucose					1772:1778	D glucose	1770:1778	D glucose	1770:1778	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	1	92	theme	different	121:129	arg1	ways					131:134	many different ways	116:134	many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies	116:245	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	4	93	theme	active	829:834	arg1	substance					836:844	the active substance	825:844	the active substance	825:844	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	94	theme	polymerization	1217:1230	arg1	degree					1232:1237	a polymerization degree	1215:1237	a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1215:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	95	dep	OBJECTIVE	678:686	arg1	allow					807:811	allow	807:811	would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation	801:985	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	96	theme	ox	1647:1648	arg1	glucose					1650:1656	ox glucose	1647:1656	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	0	97	theme	Viral	69:73	arg1	Agents					75:80	Anti- Viral Agents	63:80	Anti- Viral Agents	63:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	2	98	theme	vast	252:255	arg1	majority					257:264	The vast majority	248:264	The vast majority of ophthalmologists	248:284	The vast majority of ophthalmologists use traditional instillation of drugs in an eye disease.					
32342818	1	99	theme	effective	150:158	arg1	drugs					160:164	effective drugs	150:164	effective drugs	150:164	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	6	100	theme	carboxymethylcellulose	2060:2081	arg1	polymerization					2035:2048	polymerization	2035:2048	polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions	2035:2119	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	7	101	theme	embedded	2377:2384	arg1	nanoparticles					2386:2398	embedded nanoparticles	2377:2398	embedded nanoparticles	2377:2398	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	102	theme	±	1207:1207	arg1	substitution					1186:1197	substitution	1186:1197	substitution of 0.85 ± 2	1186:1209	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	103	theme	"	1835:1835	arg1	this					1782:1785	this	1782:1785	this	1782:1785	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	103	theme	"	1835:1835	arg1	substance					1794:1802	the substance	1790:1802	the substance of the antiviral drug "CelAgrip"	1790:1835	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	104	theme	films	1085:1089	arg1	forms					1028:1032	obtaining polymeric forms	1008:1032	obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films	1008:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	105	theme	shape	2366:2370	arg1	substance					2341:2349	a "CelAgrip" substance	2328:2349	a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm	2328:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	105	theme	shape	2366:2370	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	105	theme	shape	2366:2370	arg1	value					2426:2430	a pH value	2421:2430	a pH value of 7.6	2421:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	106	theme	derivative	1257:1266	arg1	degree					1232:1237	a polymerization degree	1215:1237	a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1215:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	106	theme	derivative	1257:1266	arg1	degree					1176:1181	a degree	1174:1181	a degree of substitution of 0.85 ± 2	1174:1209	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	107	theme	antiviral	1811:1819	arg1	"					1835:1835	the antiviral drug "CelAgrip"	1807:1835	the antiviral drug "CelAgrip"	1807:1835	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	108	theme	soluble	1041:1047	arg1	films					1085:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	3	109	dep	80	456:457	arg1	to					453:454	to	453:454	to	453:454	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	4	110	theme	forms	709:713	arg1	use					692:694	The use	688:694	The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice	688:799	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	111	theme	Nacarboxymethylcellulose	1271:1294	arg1	±					1246:1246	630 ± 20	1242:1249	630 ± 20	1242:1249	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	111	theme	Nacarboxymethylcellulose	1271:1294	arg1	derivative					1257:1266	a derivative	1255:1266	a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1255:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	3	112	theme	drip	429:432	arg1	forms					434:438	drip forms	429:438	drip forms of drugs	429:447	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	0	113	theme	Delivery	5:12	arg1	Systems					22:28	Drug Delivery Polymer Systems	0:28	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents	0:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	5	114	theme	ophthalmic	1064:1073	arg1	films					1085:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	bio soluble nanostructured ophthalmic medicinal films	1037:1089	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	1	115	theme	BACKGROUND	83:92	arg1	Ophthalmology					94:106	BACKGROUND Ophthalmology	83:106	BACKGROUND Ophthalmology	83:106	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	3	116	theme	drawbacks	380:388	arg1	number					370:375	a number	368:375	a number of drawbacks	368:388	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	5	117	theme	bound	1462:1466	arg1	polyphenol-gossypol					1476:1494	chemically bound natural polyphenol-gossypol	1451:1494	chemically bound natural polyphenol-gossypol	1451:1494	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	7	118	theme	CelAgrip	2331:2338	arg1	substance					2341:2349	a "CelAgrip" substance	2328:2349	a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm	2328:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	118	theme	CelAgrip	2331:2338	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	119	theme	±	1246:1246	arg1	degree					1232:1237	a polymerization degree	1215:1237	a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1215:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	119	theme	±	1246:1246	arg1	degree					1176:1181	a degree	1174:1181	a degree of substitution of 0.85 ± 2	1174:1209	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	6	120	dep	scission	1896:1903	arg1	time					1905:1908	time	1905:1908	time	1905:1908	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	5	121	theme	Na-carboxymethylcellulose	1134:1158	arg1	composition					1105:1115	a composition	1103:1115	a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1103:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	6	122	theme	aqueous	2103:2109	arg1	solutions					2111:2119	their aqueous solutions	2097:2119	their aqueous solutions	2097:2119	RESULT In this work the possibility of regulating the bio scission time and physicochemical parameters of ocular medicinal films has been shown, by varying the degree of substitution and degree of polymerization of sodium carboxymethylcellulose and the pH of their aqueous solutions.					
32342818	4	123	theme	instillation	974:985	arg1	effect					955:960	the negative effect	942:960	the negative effect of frequent instillation	942:985	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	4	123	theme	instillation	974:985	arg1	dosage					919:924	the dosage	915:924	the dosage of the drug	915:936	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	7	124	theme	irritating	2210:2219	arg1	effect					2221:2226	an irritating effect	2207:2226	an irritating effect	2207:2226	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	125	theme	D	1720:1720	arg1	glucose					1722:1728	D glucose -2.6	1720:1733	D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4)	1643:1763	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	126	theme	ox	1591:1592	arg1	glucose					1594:1600	ox glucose	1591:1600	2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β	1536:1639	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	0	127	theme	Ophthalmic	34:43	arg1	Administration					45:58	Ophthalmic Administration	34:58	Ophthalmic Administration of Anti- Viral Agents	34:80	Drug Delivery Polymer Systems for Ophthalmic Administration of Anti- Viral Agents.					
32342818	5	128	from	polyphenol-gossypol	1334:1352	arg1	±					1389:1389	polymerization-630 ± 20	1370:1392	polymerization-630 ± 20	1370:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	128	from	polyphenol-gossypol	1334:1352	arg1	amount					1360:1365	an amount	1357:1365	an amount of polymerization-630 ± 20	1357:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	129	theme	salt	1528:1531	arg1	-β					1765:1766	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β	1521:1766	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	1	130	dep	prevention	188:197	arg1	the					184:186	the	184:186	the	184:186	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	1	131	theme	various	228:234	arg1	etiologies					236:245	various etiologies	228:245	various etiologies	228:245	BACKGROUND Ophthalmology applies many different ways of delivering effective drugs to eye tissue for the prevention and treatment of diseases of various etiologies.					
32342818	7	132	dep	solutions	2270:2278	arg1	substance					2341:2349	a "CelAgrip" substance	2328:2349	a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm	2328:2415	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	132	dep	solutions	2270:2278	arg1	solutions					2270:2278	solutions	2270:2278	solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6	2270:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	7	132	dep	solutions	2270:2278	arg1	value					2426:2430	a pH value	2421:2430	a pH value of 7.6	2421:2437	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	4	133	theme	negative	946:953	arg1	effect					955:960	the negative effect	942:960	the negative effect of frequent instillation	942:985	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	3	134	theme	frequent	598:605	arg1	instillation					607:618	their frequent instillation	592:618	their frequent instillation	592:618	However, this method has a number of drawbacks, in particular, during instillation of drip forms of drugs, up to 80% of the drug is lost due to withdrawal of its tear fluid and rapid absorption by the mucous membrane of the eyes, which necessitates their frequent instillation to maintain the therapeutic concentration in the eyeball.					
32342818	7	135	theme	effect	2181:2186	arg1	terms					2155:2159	terms	2155:2159	terms of the prolongation effect	2155:2186	CONCLUSION The most promising in terms of the prolongation effect and the absence of an irritating effect and transparency are films, obtained from solutions of the polymer-polymer composition of Na- CMC - a "CelAgrip" substance of a spherical shape with embedded nanoparticles of size 14-52 nm and a pH value of 7.6.					
32342818	5	136	theme	mole	1514:1517	arg1	%					1518:1518	0 mole%	1512:1518	0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1512:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	137	theme	bio	718:720	arg1	films					754:758	bio soluble antiviral eye medicinal films	718:758	bio soluble antiviral eye medicinal films of prolonged form	718:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	2	138	theme	eye	330:332	arg1	disease					334:340	an eye disease	327:340	an eye disease	327:340	The vast majority of ophthalmologists use traditional instillation of drugs in an eye disease.					
32342818	5	139	theme	substitution	1186:1197	arg1	degree					1232:1237	a polymerization degree	1215:1237	a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose)	1215:1779	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	139	theme	substitution	1186:1197	arg1	degree					1176:1181	a degree	1174:1181	a degree of substitution of 0.85 ± 2	1174:1209	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	140	from	derivative	1400:1409	arg1	±					1389:1389	polymerization-630 ± 20	1370:1392	polymerization-630 ± 20	1370:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	5	140	from	derivative	1400:1409	arg1	amount					1360:1365	an amount	1357:1365	an amount of polymerization-630 ± 20	1357:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
32342818	4	141	theme	antiviral	730:738	arg1	films					754:758	bio soluble antiviral eye medicinal films	718:758	bio soluble antiviral eye medicinal films of prolonged form	718:776	OBJECTIVE The use of polymeric forms of bio soluble antiviral eye medicinal films of prolonged form in ophthalmic practice would allow introducing the active substance less often while maintaining its therapeutic concentration, reducing the dosage of the drug and the negative effect of frequent instillation.					
32342818	5	142	theme	bound	1320:1324	arg1	polyphenol-gossypol					1334:1352	a chemically bound natural polyphenol-gossypol	1307:1352	a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20	1307:1392	METHODS A method of obtaining polymeric forms of bio soluble nanostructured ophthalmic medicinal films is based on a composition of water-soluble Na-carboxymethylcellulose (Na-CMC) with a degree of substitution of 0.85 ± 2 and a polymerization degree of 630 ± 20 and a derivative of Nacarboxymethylcellulose containing a chemically bound natural polyphenol-gossypol in an amount of polymerization-630 ± 20 and a derivative of Na-carboxymethylcellulose containing chemically bound natural polyphenol-gossypol in an amount of 0 mole% (sodium salt of 2,3-diethoxy - 6 - O - carboxymethyl- (1 → 4) -β - D - ox glucose - oxy-hydroxyl - 2 - ethyl- (1 → 4) -β - D - ox glucose - diethyl hydroxyl-poly -2 - O - carboxymethyl- (1 → 4) - β - D glucose -2.6 - O -dicarboxymethyl- (1 → 4) -β - D glucose); this is the substance of the antiviral drug "CelAgrip".					
34601656	6	0	theme	chemical	796:803	arg1	composition					805:815	meat chemical composition	791:815	meat chemical composition	791:815	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	4	1	theme	experimental	506:517	arg1	treatments					519:528	three experimental treatments	500:528	three experimental treatments of four replicates per treatment 1 month to kidding	500:580	In experiment II, pregnant goat-does were randomly allocated into three experimental treatments of four replicates per treatment 1 month to kidding.					
34601656	14	2	theme	feeding	1986:1992	arg1	regimes					1994:2000	other feeding regimes	1980:2000	other feeding regimes	1980:2000	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	10	3	theme	Feed	1224:1227	arg1	efficiency					1229:1238	Feed efficiency	1224:1238	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does	1224:1317	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	15	4	theme	alternative	2054:2064	arg1	strategy					2074:2081	a suitable alternative feeding strategy	2043:2081	a suitable alternative feeding strategy	2043:2081	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	5	5	from	afternoon	631:639	arg1	fed					601:603	fed	601:603	fed	601:603	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	9	6	from	milk	1153:1156	arg1	evening-fed					1161:1171	evening-fed	1161:1171	evening-fed	1161:1171	Energy corrected milk in evening-fed does was higher (P = 0.006) than other treatments.					
34601656	8	7	from	yield	1011:1015	arg1	goat-does					1032:1040	morning-fed goat-does	1020:1040	morning-fed goat-does	1020:1040	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	11	8	theme	component	1373:1381	arg1	yield					1383:1387	Milk component yield	1368:1387	Milk component yield (g/day) for lactose (P = 0.002)	1368:1419	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	11	8	theme	component	1373:1381	arg1	highest					1429:1435	highest	1429:1435	highest	1429:1435	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	11	8	theme	component	1373:1381	arg1	g/day					1390:1394	g/day	1390:1394	g/day	1390:1394	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	1	9	theme	time	152:155	arg1	effect					142:147	the effect	138:147	the effect of time of feeding on production performance of West African Dwarf (WAD) goats	138:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	8	10	theme	morning-fed	1020:1030	arg1	goat-does					1032:1040	morning-fed goat-does	1020:1040	morning-fed goat-does	1020:1040	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	11	11	contain	had	1538:1540	arg1	afternoon-fed					1519:1531	afternoon-fed	1519:1531	afternoon-fed	1519:1531	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	11	11	contain	had	1538:1540	arg2	yield					1553:1557	the lowest yield	1542:1557	the lowest yield for all milk component parameters	1542:1591	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	13	12	theme	milk	1746:1749	arg1	yield					1751:1755	improved energy corrected milk yield	1720:1755	improved energy corrected milk yield	1720:1755	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	11	13	theme	milk	1567:1570	arg1	parameters					1582:1591	all milk component parameters	1563:1591	all milk component parameters	1563:1591	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	0	14	theme	goat	95:98	arg1	performance					61:71	the lactation and growth performance	36:71	the lactation and growth performance of West African Dwarf goat	36:98	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	11	15	dep	afternoon-fed	1519:1531	arg1	does					1533:1536	does	1533:1536	does	1533:1536	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	1	16	theme	feeding	160:166	arg1	time					152:155	time	152:155	time of feeding	152:166	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	8	17	theme	lowest	1109:1114	arg1	yield					1116:1120	the lowest yield	1105:1120	the lowest yield	1105:1120	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	14	18	theme	evening-fed	1929:1939	arg1	goats					1941:1945	evening-fed goats due to the advantage it had over other feeding regimes	1929:2000	evening-fed goats due to the advantage it had over other feeding regimes	1929:2000	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	15	19	theme	feeding	2066:2072	arg1	strategy					2074:2081	a suitable alternative feeding strategy	2043:2081	a suitable alternative feeding strategy	2043:2081	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	7	20	theme	feed	916:919	arg1	FE					933:934	FE	933:934	FE	933:934	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	7	20	theme	feed	916:919	arg1	efficiency					921:930	feed efficiency	916:930	feed efficiency (FE)	916:935	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	1	21	theme	production	171:180	arg1	performance					182:192	production performance	171:192	production performance of West African Dwarf (WAD) goats	171:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	14	22	from	productivity	1913:1924	arg1	goats					1941:1945	evening-fed goats due to the advantage it had over other feeding regimes	1929:2000	evening-fed goats due to the advantage it had over other feeding regimes	1929:2000	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	6	23	dep	affected	826:833	arg1	P > 0.05					836:843	P > 0.05	836:843	P > 0.05	836:843	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	10	24	dep	afternoon-fed	1348:1360	arg1	does					1362:1365	does	1362:1365	does	1362:1365	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	5	25	from	evening	655:661	arg1	fed					601:603	fed	601:603	fed	601:603	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	0	26	theme	lactation	40:48	arg1	performance					61:71	the lactation and growth performance	36:71	the lactation and growth performance of West African Dwarf goat	36:98	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	9	27	dep	higher	1182:1187	arg1	P = 0.006					1190:1198	P = 0.006	1190:1198	P = 0.006	1190:1198	Energy corrected milk in evening-fed does was higher (P = 0.006) than other treatments.					
34601656	10	28	theme	milk	1276:1279	arg1	P < 0.001					1288:1296	P < 0.001	1288:1296	P < 0.001	1288:1296	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	10	28	theme	milk	1276:1279	arg1	yield					1281:1285	energy corrected milk yield	1259:1285	energy corrected milk yield (P < 0.001)	1259:1297	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	0	29	theme	growth	54:59	arg1	performance					61:71	the lactation and growth performance	36:71	the lactation and growth performance of West African Dwarf goat	36:98	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	13	30	from	yield	1751:1755	arg1	goats					1790:1794	lactating goats	1780:1794	lactating goats	1780:1794	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	0	31	from	Impact	0:5	arg1	performance					61:71	the lactation and growth performance	36:71	the lactation and growth performance of West African Dwarf goat	36:98	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	10	32	theme	energy	1259:1264	arg1	P < 0.001					1288:1296	P < 0.001	1288:1296	P < 0.001	1288:1296	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	10	32	theme	energy	1259:1264	arg1	yield					1281:1285	energy corrected milk yield	1259:1285	energy corrected milk yield (P < 0.001)	1259:1297	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	11	33	theme	lowest	1546:1551	arg1	yield					1553:1557	the lowest yield	1542:1557	the lowest yield for all milk component parameters	1542:1591	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	9	34	theme	Energy	1136:1141	arg1	milk					1153:1156	Energy corrected milk	1136:1156	Energy corrected milk in evening-fed does	1136:1176	Energy corrected milk in evening-fed does was higher (P = 0.006) than other treatments.					
34601656	0	35	theme	African	81:87	arg1	goat					95:98	West African Dwarf goat	76:98	West African Dwarf goat	76:98	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	12	36	theme	production	1667:1676	arg1	yield					1692:1696	milk production and component yield	1662:1696	milk production and component yield per day	1662:1704	Conclusively, feeding in the morning and evening is recommended for milk production and component yield per day.					
34601656	11	37	contain	had	1475:1477	arg1	evening-fed					1458:1468	evening-fed	1458:1468	evening-fed	1458:1468	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	11	37	contain	had	1475:1477	arg2	fat					1509:1511	the highest (P = 0.001) crude fat	1479:1511	the highest (P = 0.001) crude fat	1479:1511	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	15	38	theme	feeding	2026:2032	arg1	time					2018:2021	time	2018:2021	time of feeding	2018:2032	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	1	39	theme	goats	222:226	arg1	performance					182:192	production performance	171:192	production performance of West African Dwarf (WAD) goats	171:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	14	40	dep	goats	1941:1945	arg1	had					1971:1973	had	1971:1973	had over other feeding regimes	1971:2000	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	0	41	theme	varied	10:15	arg1	time					17:20	varied time	10:20	varied time of feeding	10:31	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	11	42	dep	morning-fed	1440:1450	arg1	does					1452:1455	does	1452:1455	does	1452:1455	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	8	43	dep	evening-fed	1058:1068	arg1	does					1070:1073	does	1070:1073	does	1070:1073	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	5	44	from	fed	601:603	arg1	12:00 h					642:648	12:00 h	642:648	12:00 h	642:648	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	5	44	from	fed	601:603	arg1	06:00 h					621:627	06:00 h	621:627	06:00 h	621:627	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	5	44	from	fed	601:603	arg1	afternoon					631:639	afternoon	631:639	afternoon (12:00 h)	631:649	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	5	44	from	fed	601:603	arg1	morning					612:618	the morning	608:618	the morning (06:00 h)	608:628	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	5	44	from	fed	601:603	arg1	evening					655:661	evening (18:00 h)	655:671	evening (18:00 h)	655:671	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	11	45	theme	highest	1483:1489	arg1	fat					1509:1511	the highest (P = 0.001) crude fat	1479:1511	the highest (P = 0.001) crude fat	1479:1511	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	5	46	theme	light	700:704	arg1	cycles					706:711	natural light cycles	692:711	natural light cycles	692:711	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	6	47	theme	matter	735:740	arg1	intake					742:747	dry matter intake	731:747	dry matter intake	731:747	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	12	48	from	feeding	1608:1614	arg1	evening					1635:1641	evening	1635:1641	evening	1635:1641	Conclusively, feeding in the morning and evening is recommended for milk production and component yield per day.					
34601656	12	48	from	feeding	1608:1614	arg1	morning					1623:1629	morning	1623:1629	morning	1623:1629	Conclusively, feeding in the morning and evening is recommended for milk production and component yield per day.					
34601656	4	49	theme	pregnant	452:459	arg1	goat-does					461:469	pregnant goat-does	452:469	pregnant goat-does	452:469	In experiment II, pregnant goat-does were randomly allocated into three experimental treatments of four replicates per treatment 1 month to kidding.					
34601656	9	50	theme	other	1206:1210	arg1	treatments					1212:1221	other treatments	1206:1221	other treatments	1206:1221	Energy corrected milk in evening-fed does was higher (P = 0.006) than other treatments.					
34601656	4	51	theme	four replicates	533:547	arg1	treatments					519:528	three experimental treatments	500:528	three experimental treatments of four replicates per treatment 1 month to kidding	500:580	In experiment II, pregnant goat-does were randomly allocated into three experimental treatments of four replicates per treatment 1 month to kidding.					
34601656	11	52	theme	crude	1503:1507	arg1	fat					1509:1511	the highest (P = 0.001) crude fat	1479:1511	the highest (P = 0.001) crude fat	1479:1511	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	3	53	dep	five	396:399	arg1	replicates					401:410	replicates	401:410	replicates	401:410	In Experiment I, the bucks were randomly allocated into three treatments of five replicates and fed for 115 days.					
34601656	10	54	from	efficiency	1229:1238	arg1	evening-fed					1302:1312	evening-fed	1302:1312	evening-fed	1302:1312	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	4	55	theme	treatment	553:561	arg1	1 month					563:569	treatment 1 month	553:569	treatment 1 month to kidding	553:580	In experiment II, pregnant goat-does were randomly allocated into three experimental treatments of four replicates per treatment 1 month to kidding.					
34601656	12	56	dep	morning	1623:1629	arg1	the					1619:1621	the	1619:1621	the	1619:1621	Conclusively, feeding in the morning and evening is recommended for milk production and component yield per day.					
34601656	15	57	with	climate	2097:2103	arg1	potential					2112:2120	a potential to improve production efficiency	2110:2153	a potential to improve production efficiency	2110:2153	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	1	58	theme	Dwarf	210:214	arg1	goats					222:226	West African Dwarf (WAD) goats	197:226	West African Dwarf (WAD) goats	197:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	6	59	theme	meat	791:794	arg1	composition					805:815	meat chemical composition	791:815	meat chemical composition	791:815	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	14	60	theme	milk	1908:1911	arg1	productivity					1913:1924	milk productivity	1908:1924	milk productivity in evening-fed goats due to the advantage it had over other feeding regimes	1908:2000	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	14	61	theme	other	1980:1984	arg1	regimes					1994:2000	other feeding regimes	1980:2000	other feeding regimes	1980:2000	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	13	62	theme	improved	1720:1727	arg1	yield					1751:1755	improved energy corrected milk yield	1720:1755	improved energy corrected milk yield	1720:1755	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	7	63	theme	milk	942:945	arg1	yield					947:951	milk yield	942:951	milk yield	942:951	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	11	64	theme	Milk	1368:1371	arg1	yield					1383:1387	Milk component yield	1368:1387	Milk component yield (g/day) for lactose (P = 0.002)	1368:1419	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	11	64	theme	Milk	1368:1371	arg1	highest					1429:1435	highest	1429:1435	highest	1429:1435	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	11	64	theme	Milk	1368:1371	arg1	g/day					1390:1394	g/day	1390:1394	g/day	1390:1394	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	1	65	theme	WAD	217:219	arg1	goats					222:226	West African Dwarf (WAD) goats	197:226	West African Dwarf (WAD) goats	197:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	8	66	contain	have	1100:1103	arg2	yield					1116:1120	the lowest yield	1105:1120	the lowest yield	1105:1120	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	8	66	contain	have	1100:1103	arg1	afternoon-fed					1081:1093	afternoon-fed	1081:1093	afternoon-fed	1081:1093	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	13	67	theme	feed	1761:1764	arg1	efficiency					1766:1775	feed efficiency	1761:1775	feed efficiency	1761:1775	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	6	68	theme	carcass	763:769	arg1	characteristic					771:784	carcass characteristic	763:784	carcass characteristic	763:784	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	10	69	theme	milk	1244:1247	arg1	yield					1249:1253	milk yield	1244:1253	milk yield	1244:1253	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	13	70	from	efficiency	1766:1775	arg1	goats					1790:1794	lactating goats	1780:1794	lactating goats	1780:1794	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	8	71	theme	milk	1006:1009	arg1	yield					1011:1015	milk yield	1006:1015	milk yield in morning-fed goat-does	1006:1040	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	13	72	theme	energy	1729:1734	arg1	yield					1751:1755	improved energy corrected milk yield	1720:1755	improved energy corrected milk yield	1720:1755	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	13	73	theme	corrected	1736:1744	arg1	yield					1751:1755	improved energy corrected milk yield	1720:1755	improved energy corrected milk yield	1720:1755	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	11	74	theme	component	1572:1580	arg1	parameters					1582:1591	all milk component parameters	1563:1591	all milk component parameters	1563:1591	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	15	75	from	strategy	2074:2081	arg1	tropics					2174:2180	the tropics	2170:2180	the tropics	2170:2180	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	15	75	from	strategy	2074:2081	arg1	climate					2097:2103	a changing climate	2086:2103	a changing climate with a potential to improve production efficiency	2086:2153	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	7	76	theme	feed	903:906	arg1	intake					908:913	feed intake	903:913	feed intake	903:913	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	15	77	theme	production	2133:2142	arg1	efficiency					2144:2153	production efficiency	2133:2153	production efficiency	2133:2153	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	7	78	theme	data	884:887	arg1	collection					889:898	data collection	884:898	data collection on feed intake, feed efficiency (FE), and milk yield	884:951	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	2	79	dep	goats	268:272	arg1	goats					268:272	twenty-seven goats	255:272	twenty-seven goats (15 bucks and 12 gravid does)	255:302	Two experiments involving twenty-seven goats (15 bucks and 12 gravid does) were conducted.					
34601656	2	79	dep	goats	268:272	arg1	bucks					278:282	15 bucks	275:282	15 bucks	275:282	Two experiments involving twenty-seven goats (15 bucks and 12 gravid does) were conducted.					
34601656	2	79	dep	goats	268:272	arg1	gravid					291:296	12 gravid does	288:301	12 gravid does	288:301	Two experiments involving twenty-seven goats (15 bucks and 12 gravid does) were conducted.					
34601656	15	80	theme	changing	2088:2095	arg1	climate					2097:2103	a changing climate	2086:2103	a changing climate with a potential to improve production efficiency	2086:2153	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	1	81	from	effect	142:147	arg1	performance					182:192	production performance	171:192	production performance of West African Dwarf (WAD) goats	171:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	9	82	dep	evening-fed	1161:1171	arg1	does					1173:1176	does	1173:1176	does	1173:1176	Energy corrected milk in evening-fed does was higher (P = 0.006) than other treatments.					
34601656	10	83	theme	corrected	1266:1274	arg1	P < 0.001					1288:1296	P < 0.001	1288:1296	P < 0.001	1288:1296	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	10	83	theme	corrected	1266:1274	arg1	yield					1281:1285	energy corrected milk yield	1259:1285	energy corrected milk yield (P < 0.001)	1259:1297	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	10	84	dep	evening-fed	1302:1312	arg1	does					1314:1317	does	1314:1317	does	1314:1317	Feed efficiency for milk yield and energy corrected milk yield (P < 0.001) in evening-fed does were higher than morning and afternoon-fed does.					
34601656	6	85	theme	feeding	857:863	arg1	time					849:852	time	849:852	time of feeding	849:863	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	2	86	dep	gravid	291:296	arg1	does					298:301	does	298:301	does	298:301	Two experiments involving twenty-seven goats (15 bucks and 12 gravid does) were conducted.					
34601656	0	87	theme	Dwarf	89:93	arg1	goat					95:98	West African Dwarf goat	76:98	West African Dwarf goat	76:98	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	9	88	theme	corrected	1143:1151	arg1	milk					1153:1156	Energy corrected milk	1136:1156	Energy corrected milk in evening-fed does	1136:1176	Energy corrected milk in evening-fed does was higher (P = 0.006) than other treatments.					
34601656	12	89	theme	and component	1678:1690	arg1	yield					1692:1696	milk production and component yield	1662:1696	milk production and component yield per day	1662:1704	Conclusively, feeding in the morning and evening is recommended for milk production and component yield per day.					
34601656	8	90	dep	afternoon-fed	1081:1093	arg1	does					1095:1098	does	1095:1098	does	1095:1098	From the results, milk yield in morning-fed goat-does was higher than evening-fed does while afternoon-fed does have the lowest yield at P < 0.001.					
34601656	14	91	theme	further	1859:1865	arg1	studies					1867:1873	further studies	1859:1873	further studies	1859:1873	It is recommended that further studies should be conducted on improving milk productivity in evening-fed goats due to the advantage it had over other feeding regimes.					
34601656	0	92	theme	time	17:20	arg1	Impact					0:5	Impact	0:5	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.	0:99	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	3	93	theme	five	396:399	arg1	treatments					382:391	three treatments	376:391	three treatments of five replicates	376:410	In Experiment I, the bucks were randomly allocated into three treatments of five replicates and fed for 115 days.					
34601656	0	94	theme	feeding	25:31	arg1	time					17:20	varied time	10:20	varied time of feeding	10:31	Impact of varied time of feeding on the lactation and growth performance of West African Dwarf goat.					
34601656	12	95	theme	milk	1662:1665	arg1	yield					1692:1696	milk production and component yield	1662:1696	milk production and component yield per day	1662:1704	Conclusively, feeding in the morning and evening is recommended for milk production and component yield per day.					
34601656	6	96	theme	weight	750:755	arg1	gain					757:760	weight gain	750:760	weight gain	750:760	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	5	97	theme	natural	692:698	arg1	cycles					706:711	natural light cycles	692:711	natural light cycles	692:711	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
34601656	11	98	dep	evening-fed	1458:1468	arg1	does					1470:1473	does	1470:1473	does	1470:1473	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	6	99	theme	dry	731:733	arg1	intake					742:747	dry matter intake	731:747	dry matter intake	731:747	In experiment I, dry matter intake, weight gain, carcass characteristic, and meat chemical composition were not affected (P > 0.05) by time of feeding.					
34601656	15	100	theme	suitable	2045:2052	arg1	strategy					2074:2081	a suitable alternative feeding strategy	2043:2081	a suitable alternative feeding strategy	2043:2081	Thus, altering time of feeding could be a suitable alternative feeding strategy in a changing climate with a potential to improve production efficiency, especially in the tropics.					
34601656	7	101	from	collection	889:898	arg1	yield					947:951	milk yield	942:951	milk yield	942:951	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	7	101	from	collection	889:898	arg1	FE					933:934	FE	933:934	FE	933:934	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	7	101	from	collection	889:898	arg1	intake					908:913	feed intake	903:913	feed intake	903:913	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	7	101	from	collection	889:898	arg1	efficiency					921:930	feed efficiency	916:930	feed efficiency (FE)	916:935	In experiment II, data collection on feed intake, feed efficiency (FE), and milk yield spanned 6 weeks and were analyzed.					
34601656	11	102	dep	highest	1483:1489	arg1	P = 0.001					1492:1500	P = 0.001	1492:1500	P = 0.001	1492:1500	Milk component yield (g/day) for lactose (P = 0.002) was the highest in morning-fed does; evening-fed does had the highest (P = 0.001) crude fat while afternoon-fed does had the lowest yield for all milk component parameters.					
34601656	13	103	from	feeding	1797:1803	arg1	evening					1812:1818	the evening	1808:1818	the evening	1808:1818	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	1	104	theme	African	202:208	arg1	goats					222:226	West African Dwarf (WAD) goats	197:226	West African Dwarf (WAD) goats	197:226	This study was conducted to evaluate the effect of time of feeding on production performance of West African Dwarf (WAD) goats.					
34601656	13	105	theme	lactating	1780:1788	arg1	goats					1790:1794	lactating goats	1780:1794	lactating goats	1780:1794	However, for improved energy corrected milk yield and feed efficiency in lactating goats, feeding in the evening is recommended.					
34601656	5	106	from	morning	612:618	arg1	fed					601:603	fed	601:603	fed	601:603	Goats were either fed in the morning (06:00 h), afternoon (12:00 h), or evening (18:00 h) respectively under natural light cycles.					
32661246	0	0	theme	genes	69:73	arg1	expression					35:44	differential expression	22:44	differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	22:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	0	1	theme	crispus	161:167	arg1	stages					129:134	the gametophyte and tetasporophyte life cycle stages	83:134	the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	83:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	1	2	with	alga	203:206	arg1	galactans					222:230	sulfated galactans	213:230	sulfated galactans	213:230	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	4	3	theme	carrageenan	681:691	arg1	structures					693:702	carrageenan structures	681:702	carrageenan structures	681:702	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	4	4	theme	differential	767:778	arg1	regulation					780:789	differential regulation	767:789	differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles	767:864	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	8	5	theme	cell	1553:1556	arg1	biosynthesis					1563:1574	cell wall biosynthesis	1553:1574	cell wall biosynthesis in algae	1553:1583	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	0	6	theme	carrageenan-related	49:67	arg1	genes					69:73	carrageenan-related genes	49:73	carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	49:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	0	7	theme	Chondrus	152:159	arg1	crispus					161:167	the red alga Chondrus crispus	139:167	the red alga Chondrus crispus	139:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	5	8	theme	biochemistry	902:913	arg1	combination					877:887	a combination	875:887	a combination of taxonomy, biochemistry and molecular biology	875:935	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	4	9	from	disparity	668:676	arg1	structures					693:702	carrageenan structures	681:702	carrageenan structures	681:702	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	5	10	dep	coast	1083:1087	arg1	off					1066:1068	off	1066:1068	off	1066:1068	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	9	11	theme	Chondrus	1753:1760	arg1	stages					1743:1748	the isomorphic life cycle stages	1717:1748	the isomorphic life cycle stages of Chondrus	1717:1760	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	8	12	theme	rare	1535:1538	arg1	glimpse					1540:1546	a rare glimpse	1533:1546	a rare glimpse into cell wall biosynthesis in algae	1533:1583	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	5	13	theme	male	978:981	arg1	gametophytes					994:1005	male and female gametophytes	978:1005	male and female gametophytes	978:1005	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	1	14	from	alga	203:206	arg1	matrix					275:280	its extracellular matrix	257:280	its extracellular matrix	257:280	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	5	15	attach	isolated	1033:1040	arg1	seashore					1057:1064	the rocky seashore	1047:1064	the rocky seashore off the northern coast of France	1047:1097	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	5	15	attach	isolated	1033:1040	arg2	individuals					1021:1031	Chondrus individuals	1012:1031	Chondrus individuals isolated from the rocky seashore off the northern coast of France	1012:1097	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	8	16	theme	differential	1474:1485	arg1	expression					1487:1496	The differential expression	1470:1496	The differential expression of these multigenic genes	1470:1522	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	7	17	theme	carrageenan-related	1330:1348	arg1	genes					1350:1354	carrageenan-related genes	1330:1354	carrageenan-related genes	1330:1354	Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes.					
32661246	9	18	theme	cycle	1737:1741	arg1	stages					1743:1748	the isomorphic life cycle stages	1717:1748	the isomorphic life cycle stages of Chondrus	1717:1760	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	5	19	from	individuals	1021:1031	arg1	tetrasporophytes					957:972	tetrasporophytes	957:972	tetrasporophytes	957:972	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	5	19	from	individuals	1021:1031	arg1	gametophytes					994:1005	male and female gametophytes	978:1005	male and female gametophytes	978:1005	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	7	20	theme	genes	1350:1354	arg1	expression					1316:1325	Differential expression	1303:1325	Differential expression of carrageenan-related genes	1303:1354	Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes.					
32661246	4	21	theme	life	854:857	arg1	cycles					859:864	the Chondrus life cycles	841:864	the Chondrus life cycles	841:864	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	3	22	theme	major	471:475	arg1	difference					488:497	a major phenotypic difference	469:497	a major phenotypic difference	469:497	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	5	23	theme	biology	929:935	arg1	combination					877:887	a combination	875:887	a combination of taxonomy, biochemistry and molecular biology	875:935	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	6	24	theme	genes	1163:1167	arg1	expression					1149:1158	differential gene expression	1131:1158	differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase	1131:1300	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	0	25	theme	gametophyte	87:97	arg1	stages					129:134	the gametophyte and tetasporophyte life cycle stages	83:134	the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	83:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	4	26	theme	Chondrus	845:852	arg1	cycles					859:864	the Chondrus life cycles	841:864	the Chondrus life cycles	841:864	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	3	27	dep	tetrasporophytes	564:579	arg1	lambda-carrageenan					589:606	lambda-carrageenan	589:606	lambda-carrageenan	589:606	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	3	28	theme	phenotypic	477:486	arg1	difference					488:497	a major phenotypic difference	469:497	a major phenotypic difference	469:497	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	2	29	theme	female	362:367	arg1	n					383:383	n	383:383	n	383:383	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	2	29	theme	female	362:367	arg1	gametophytes					369:380	male and female gametophytes	353:380	male and female gametophytes (n)	353:384	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	4	30	theme	chemical	728:735	arg1	properties					737:746	different chemical properties	718:746	different chemical properties	718:746	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	9	31	theme	physiological	1679:1691	arg1	differentiation					1693:1707	the physiological differentiation	1675:1707	the physiological differentiation between the isomorphic life cycle stages of Chondrus	1675:1760	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	4	32	theme	cycles	859:864	arg1	phases					831:836	the phases	827:836	the phases of the Chondrus life cycles	827:864	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	4	33	theme	different	718:726	arg1	properties					737:746	different chemical properties	718:746	different chemical properties	718:746	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	1	34	theme	Chondrus	170:177	arg1	alga					203:206	a marine red alga	190:206	a marine red alga	190:206	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	1	34	theme	Chondrus	170:177	arg1	crispus					179:185	Chondrus crispus	170:185	Chondrus crispus	170:185	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	6	35	theme	family	1272:1277	arg1	hydrolase					1292:1300	one family 16 glycoside hydrolase	1268:1300	one family 16 glycoside hydrolase	1268:1300	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	0	36	theme	life	118:121	arg1	stages					129:134	the gametophyte and tetasporophyte life cycle stages	83:134	the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	83:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	7	37	theme	female	1449:1454	arg1	gametophytes					1456:1467	the male and female gametophytes	1436:1467	the male and female gametophytes	1436:1467	Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes.					
32661246	5	38	used	used	870:873	arg2	We					867:868	We	867:868	We	867:868	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	9	39	theme	isomorphic	1721:1730	arg1	stages					1743:1748	the isomorphic life cycle stages	1717:1748	the isomorphic life cycle stages of Chondrus	1717:1760	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	3	40	theme	Chondrus	417:424	arg1	isomorphic					448:457	isomorphic	448:457	isomorphic	448:457	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	3	40	theme	Chondrus	417:424	arg1	stages					437:442	The Chondrus life cycle stages	413:442	The Chondrus life cycle stages	413:442	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	0	41	theme	tetasporophyte	103:116	arg1	stages					129:134	the gametophyte and tetasporophyte life cycle stages	83:134	the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	83:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	8	42	theme	wall	1558:1561	arg1	biosynthesis					1563:1574	cell wall biosynthesis	1553:1574	cell wall biosynthesis in algae	1553:1583	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	3	43	theme	carrageenan	507:517	arg1	composition					519:529	carrageenan composition	507:529	carrageenan composition	507:529	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	3	44	theme	life	426:429	arg1	isomorphic					448:457	isomorphic	448:457	isomorphic	448:457	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	3	44	theme	life	426:429	arg1	stages					437:442	The Chondrus life cycle stages	413:442	The Chondrus life cycle stages	413:442	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	1	45	theme	extracellular	261:273	arg1	matrix					275:280	its extracellular matrix	257:280	its extracellular matrix	257:280	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	5	46	theme	France	1092:1097	arg1	coast					1083:1087	the northern coast	1070:1087	the northern coast of France	1070:1097	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	8	47	theme	multigenic	1507:1516	arg1	genes					1518:1522	these multigenic genes	1501:1522	these multigenic genes	1501:1522	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	2	48	theme	male	353:356	arg1	n					383:383	n	383:383	n	383:383	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	2	48	theme	male	353:356	arg1	gametophytes					369:380	male and female gametophytes	353:380	male and female gametophytes (n)	353:384	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	6	49	theme	Transcriptomic	1100:1113	arg1	analyses					1115:1122	Transcriptomic analyses	1100:1122	Transcriptomic analyses	1100:1122	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	0	50	theme	cycle	123:127	arg1	stages					129:134	the gametophyte and tetasporophyte life cycle stages	83:134	the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	83:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	5	51	theme	molecular	919:927	arg1	biology					929:935	molecular biology	919:935	molecular biology	919:935	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	1	52	theme	marine	192:197	arg1	alga					203:206	a marine red alga	190:206	a marine red alga	190:206	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	1	52	theme	marine	192:197	arg1	crispus					179:185	Chondrus crispus	170:185	Chondrus crispus	170:185	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	0	53	theme	differential	22:33	arg1	expression					35:44	differential expression	22:44	differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	22:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	5	54	theme	rocky	1051:1055	arg1	seashore					1057:1064	the rocky seashore	1047:1064	the rocky seashore off the northern coast of France	1047:1097	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	6	55	theme	gene	1144:1147	arg1	expression					1149:1158	differential gene expression	1131:1158	differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase	1131:1300	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	2	56	theme	life	321:324	arg1	cycle					326:330	a complex haplodiplontic life cycle	296:330	a complex haplodiplontic life cycle	296:330	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	9	57	theme	important	1657:1665	arg1	role					1667:1670	an important role	1654:1670	an important role	1654:1670	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	8	58	theme	genes	1518:1522	arg1	expression					1487:1496	The differential expression	1470:1496	The differential expression of these multigenic genes	1470:1522	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	2	59	theme	haplodiplontic	306:319	arg1	cycle					326:330	a complex haplodiplontic life cycle	296:330	a complex haplodiplontic life cycle	296:330	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	2	60	contain	has	292:294	arg1	Chondrus					283:290	Chondrus	283:290	Chondrus	283:290	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	2	60	contain	has	292:294	arg2	cycle					326:330	a complex haplodiplontic life cycle	296:330	a complex haplodiplontic life cycle	296:330	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	0	61	theme	alga	147:150	arg1	crispus					161:167	the red alga Chondrus crispus	139:167	the red alga Chondrus crispus	139:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	6	62	theme	glycoside	1282:1290	arg1	hydrolase					1292:1300	one family 16 glycoside hydrolase	1268:1300	one family 16 glycoside hydrolase	1268:1300	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	4	63	theme	carrageenan-active	794:811	arg1	genes					813:817	carrageenan-active genes	794:817	carrageenan-active genes	794:817	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	9	64	theme	life	1732:1735	arg1	stages					1743:1748	the isomorphic life cycle stages	1717:1748	the isomorphic life cycle stages of Chondrus	1717:1760	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	2	65	theme	complex	298:304	arg1	cycle					326:330	a complex haplodiplontic life cycle	296:330	a complex haplodiplontic life cycle	296:330	Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n).					
32661246	1	66	theme	red	199:201	arg1	alga					203:206	a marine red alga	190:206	a marine red alga	190:206	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	1	66	theme	red	199:201	arg1	crispus					179:185	Chondrus crispus	170:185	Chondrus crispus	170:185	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
32661246	0	67	theme	red	143:145	arg1	crispus					161:167	the red alga Chondrus crispus	139:167	the red alga Chondrus crispus	139:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	5	68	theme	northern	1074:1081	arg1	coast					1083:1087	the northern coast	1070:1087	the northern coast of France	1070:1097	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	5	69	theme	Chondrus	1012:1019	arg1	individuals					1021:1031	Chondrus individuals	1012:1031	Chondrus individuals isolated from the rocky seashore off the northern coast of France	1012:1097	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	6	70	theme	differential	1131:1142	arg1	expression					1149:1158	differential gene expression	1131:1158	differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase	1131:1300	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	3	71	theme	cycle	431:435	arg1	isomorphic					448:457	isomorphic	448:457	isomorphic	448:457	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	3	71	theme	cycle	431:435	arg1	stages					437:442	The Chondrus life cycle stages	413:442	The Chondrus life cycle stages	413:442	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	7	72	theme	Differential	1303:1314	arg1	expression					1316:1325	Differential expression	1303:1325	Differential expression of carrageenan-related genes	1303:1354	Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes.					
32661246	5	73	theme	female	987:992	arg1	gametophytes					994:1005	male and female gametophytes	978:1005	male and female gametophytes	978:1005	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	0	74	dep	gel	3:5	arg1	expression					35:44	differential expression	22:44	differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus	22:167	To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.					
32661246	9	75	theme	carrageenan	1625:1635	arg1	metabolism					1637:1646	carrageenan metabolism	1625:1646	carrageenan metabolism	1625:1646	Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.					
32661246	5	76	dep	tetrasporophytes	957:972	arg1	the					953:955	the	953:955	the	953:955	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	7	77	theme	male	1440:1443	arg1	gametophytes					1456:1467	the male and female gametophytes	1436:1467	the male and female gametophytes	1436:1467	Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes.					
32661246	3	78	dep	gametophytes	617:628	arg1	kappa/iota-carrageenans					638:660	kappa/iota-carrageenans	638:660	kappa/iota-carrageenans	638:660	The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans).					
32661246	8	79	from	biosynthesis	1563:1574	arg1	algae					1579:1583	algae	1579:1583	algae	1579:1583	The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae.					
32661246	6	80	theme	several	1178:1184	arg1	galactose-sulfurylases					1186:1207	several galactose-sulfurylases	1178:1207	several galactose-sulfurylases	1178:1207	Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase.					
32661246	4	81	theme	genes	813:817	arg1	regulation					780:789	differential regulation	767:789	differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles	767:864	The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles.					
32661246	5	82	theme	taxonomy	892:899	arg1	combination					877:887	a combination	875:887	a combination of taxonomy, biochemistry and molecular biology	875:935	We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France.					
32661246	1	83	theme	sulfated	213:220	arg1	galactans					222:230	sulfated galactans	213:230	sulfated galactans	213:230	Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix.					
33908003	0	0	theme	Hydrophilic	91:101	arg1	Chromatography					115:128	Hydrophilic Interaction Chromatography	91:128	Hydrophilic Interaction Chromatography	91:128	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	6	1	theme	electrostatic	984:996	arg1	repulsion					998:1006	electrostatic repulsion	984:1006	electrostatic repulsion	984:1006	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	4	2	gly	glycopeptides	701:713	arg2	glycopeptides					701:713	glycopeptides	701:713	glycopeptides	701:713	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	4	3	gly	nonglycosylated	748:762	arg1	peptides					764:771	nonglycosylated peptides	748:771	nonglycosylated peptides	748:771	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	1	4	theme	protein	185:191	arg1	modification					211:222	a biologically important and complex protein posttranslational modification	148:222	a biologically important and complex protein posttranslational modification	148:222	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	1	4	theme	protein	185:191	arg1	Glycosylation					131:143	Glycosylation	131:143	Glycosylation	131:143	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	9	5	theme	glycans	1506:1512	arg1	cleavage					1494:1501	cleavage	1494:1501	cleavage of glycans prior to LC-MS/MS analysis	1494:1539	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	9	6	dep	robust	1448:1453	arg1	easy					1456:1459	easy	1456:1459	easy	1456:1459	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	9	6	dep	robust	1448:1453	arg1	robust					1448:1453	robust	1448:1453	robust	1448:1453	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	9	6	dep	robust	1448:1453	arg1	strategy					1436:1443	The enrichment strategy	1421:1443	The enrichment strategy	1421:1443	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	7	7	theme	subsequent	1224:1233	arg1	analysis					1244:1251	subsequent LC-MS/MS analysis	1224:1251	subsequent LC-MS/MS analysis	1224:1251	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	1	8	theme	posttranslational	193:209	arg1	modification					211:222	a biologically important and complex protein posttranslational modification	148:222	a biologically important and complex protein posttranslational modification	148:222	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	1	8	theme	posttranslational	193:209	arg1	Glycosylation					131:143	Glycosylation	131:143	Glycosylation	131:143	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	2	9	from	role	374:377	arg1	areas					434:438	other areas	428:438	other areas of interest	428:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	9	from	role	374:377	arg1	biology					399:405	biology	399:405	biology	399:405	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	9	from	role	374:377	arg1	states					416:421	disease states	408:421	disease states	408:421	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	9	10	theme	prior	1514:1518	arg1	glycans					1506:1512	glycans	1506:1512	glycans prior to LC-MS/MS analysis	1506:1539	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	5	11	theme	glycopeptides	788:800	arg1	Enrichment					774:783	Enrichment	774:783	Enrichment of glycopeptides from a protein enzymatic digest	774:832	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	5	11	theme	glycopeptides	788:800	arg1	approach					850:857	an effective approach	837:857	an effective approach to overcome such challenges	837:885	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	7	12	theme	biological	1200:1209	arg1	samples					1211:1217	biological samples	1200:1217	biological samples	1200:1217	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	2	13	contain	has	319:321	arg2	potential					327:335	the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest	323:450	the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest	323:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	13	contain	has	319:321	arg1	emergence					229:237	The emergence	225:237	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins	225:317	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	0	14	theme	Interaction	103:113	arg1	Chromatography					115:128	Hydrophilic Interaction Chromatography	91:128	Hydrophilic Interaction Chromatography	91:128	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	6	15	theme	anion	968:972	arg1	exchange					974:981	strong anion exchange	961:981	strong anion exchange	961:981	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	2	16	theme	disease	408:414	arg1	states					416:421	disease states	408:421	disease states	408:421	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	9	17	theme	LC-MS/MS	1523:1530	arg1	analysis					1532:1539	LC-MS/MS analysis	1523:1539	LC-MS/MS analysis	1523:1539	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	6	18	theme	strong	961:966	arg1	exchange					974:981	strong anion exchange	961:981	strong anion exchange	961:981	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	8	19	theme	identifications	1367:1381	arg1	number					1327:1332	a high number	1320:1332	a high number of total and unique glycopeptide identifications which can be mapped back to proteins	1320:1418	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	4	20	theme	complex	720:726	arg1	mixture					728:734	a complex mixture	718:734	a complex mixture relative to nonglycosylated peptides	718:771	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	7	21	from	samples	1211:1217	arg1	glycopeptides					1181:1193	glycopeptides	1181:1193	glycopeptides from biological samples	1181:1217	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	5	22	theme	protein	809:815	arg1	digest					827:832	a protein enzymatic digest	807:832	a protein enzymatic digest	807:832	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	5	23	theme	effective	840:848	arg1	Enrichment					774:783	Enrichment	774:783	Enrichment of glycopeptides from a protein enzymatic digest	774:832	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	5	23	theme	effective	840:848	arg1	approach					850:857	an effective approach	837:857	an effective approach to overcome such challenges	837:885	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	4	24	theme	nonglycosylated	748:762	arg1	peptides					764:771	nonglycosylated peptides	748:771	nonglycosylated peptides	748:771	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	2	25	from	glycans	299:305	arg1	proteins					310:317	proteins	310:317	proteins	310:317	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	4	26	theme	glycopeptides	701:713	arg1	abundance					688:696	the low abundance	680:696	the low abundance of glycopeptides	680:713	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	4	26	theme	glycopeptides	701:713	arg1	diversity					666:674	extensive glycan diversity	649:674	extensive glycan diversity	649:674	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	5	27	theme	such	871:874	arg1	challenges					876:885	such challenges	871:885	such challenges	871:885	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	5	28	theme	enzymatic	817:825	arg1	digest					827:832	a protein enzymatic digest	807:832	a protein enzymatic digest	807:832	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	9	29	theme	enrichment	1425:1434	arg1	easy					1456:1459	easy	1456:1459	easy	1456:1459	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	9	29	theme	enrichment	1425:1434	arg1	robust					1448:1453	robust	1448:1453	robust	1448:1453	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	9	29	theme	enrichment	1425:1434	arg1	strategy					1436:1443	The enrichment strategy	1421:1443	The enrichment strategy	1421:1443	The enrichment strategy is robust, easy to perform, and does not require cleavage of glycans prior to LC-MS/MS analysis.					
33908003	2	30	theme	other	428:432	arg1	areas					434:438	other areas	428:438	other areas of interest	428:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	8	31	theme	SAX-ERLIC	1292:1300	arg1	enrichment					1302:1311	the SAX-ERLIC enrichment	1288:1311	the SAX-ERLIC enrichment	1288:1311	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	5	32	from	digest	827:832	arg1	Enrichment					774:783	Enrichment	774:783	Enrichment of glycopeptides from a protein enzymatic digest	774:832	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	5	32	from	digest	827:832	arg1	approach					850:857	an effective approach	837:857	an effective approach to overcome such challenges	837:885	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	8	33	theme	Glycopeptide	1254:1265	arg1	data					1267:1270	Glycopeptide data	1254:1270	Glycopeptide data generated using the SAX-ERLIC enrichment	1254:1311	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	5	34	gly	glycopeptides	788:800	arg2	glycopeptides					788:800	glycopeptides	788:800	glycopeptides	788:800	Enrichment of glycopeptides from a protein enzymatic digest is an effective approach to overcome such challenges.					
33908003	6	35	gly	glycopeptide	920:931	arg2	glycopeptide					920:931	glycopeptide	920:931	glycopeptide	920:931	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	8	36	theme	glycopeptide	1354:1365	arg1	identifications					1367:1381	total and unique glycopeptide identifications	1337:1381	total and unique glycopeptide identifications which can be mapped back to proteins	1337:1418	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	0	37	theme	Glycopeptides	21:33	arg1	Enrichment					0:9	Enrichment	0:9	Enrichment of Intact Glycopeptides	0:33	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	8	38	theme	unique	1347:1352	arg1	identifications					1367:1381	total and unique glycopeptide identifications	1337:1381	total and unique glycopeptide identifications which can be mapped back to proteins	1337:1418	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	7	39	theme	phase	1154:1158	arg1	extraction					1160:1169	solid phase extraction	1148:1169	solid phase extraction	1148:1169	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	4	40	from	abundance	688:696	arg1	mixture					728:734	a complex mixture	718:734	a complex mixture relative to nonglycosylated peptides	718:771	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	4	41	theme	low	684:686	arg1	abundance					688:696	the low abundance	680:696	the low abundance of glycopeptides	680:713	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	0	42	theme	Intact	14:19	arg1	Glycopeptides					21:33	Intact Glycopeptides	14:33	Intact Glycopeptides	14:33	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	6	43	theme	enrichment	933:942	arg1	method					944:949	a glycopeptide enrichment method	918:949	a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC)	918:1062	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	2	44	dep	enable	340:345	arg1	understanding					356:368	understanding	356:368	understanding the role of glycosylation in biology, disease states, and other areas of interest	356:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	3	45	theme	mass	508:511	arg1	spectrometry					513:524	mass spectrometry	508:524	mass spectrometry	508:524	In particular, the analysis of intact glycopeptides by mass spectrometry allows information about glycan location and composition to be ascertained.					
33908003	0	46	theme	Strong	41:46	arg1	Exchange					54:61	Strong Anion Exchange	41:61	Strong Anion Exchange	41:61	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	4	47	theme	such	611:614	arg1	analysis					616:623	such analysis	611:623	such analysis	611:623	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	6	48	theme	glycopeptide	920:931	arg1	method					944:949	a glycopeptide enrichment method	918:949	a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC)	918:1062	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	0	49	dep	Exchange	54:61	arg1	Chromatography					115:128	Hydrophilic Interaction Chromatography	91:128	Hydrophilic Interaction Chromatography	91:128	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	3	50	theme	intact	484:489	arg1	glycopeptides					491:503	intact glycopeptides	484:503	intact glycopeptides	484:503	In particular, the analysis of intact glycopeptides by mass spectrometry allows information about glycan location and composition to be ascertained.					
33908003	8	51	theme	high	1322:1325	arg1	number					1327:1332	a high number	1320:1332	a high number of total and unique glycopeptide identifications which can be mapped back to proteins	1320:1418	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	6	52	theme	interaction	1025:1035	arg1	SAX-ERLIC					1053:1061	SAX-ERLIC	1053:1061	SAX-ERLIC	1053:1061	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	6	52	theme	interaction	1025:1035	arg1	chromatography					1037:1050	hydrophilic interaction chromatography	1013:1050	hydrophilic interaction chromatography (SAX-ERLIC)	1013:1062	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	7	53	theme	solid	1148:1152	arg1	extraction					1160:1169	solid phase extraction	1148:1169	solid phase extraction	1148:1169	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	8	54	gly	glycopeptide	1354:1365	arg2	glycopeptide					1354:1365	total and unique glycopeptide identifications	1337:1381	total and unique glycopeptide identifications which can be mapped back to proteins	1337:1418	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	2	55	theme	interest	443:450	arg1	areas					434:438	other areas	428:438	other areas of interest	428:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	55	theme	interest	443:450	arg1	biology					399:405	biology	399:405	biology	399:405	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	55	theme	interest	443:450	arg1	states					416:421	disease states	408:421	disease states	408:421	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	7	56	theme	LC-MS/MS	1235:1242	arg1	analysis					1244:1251	subsequent LC-MS/MS analysis	1224:1251	subsequent LC-MS/MS analysis	1224:1251	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	3	57	theme	glycan	551:556	arg1	location					558:565	glycan location	551:565	glycan location	551:565	In particular, the analysis of intact glycopeptides by mass spectrometry allows information about glycan location and composition to be ascertained.					
33908003	3	58	gly	glycopeptides	491:503	arg2	glycopeptides					491:503	intact glycopeptides	484:503	intact glycopeptides	484:503	In particular, the analysis of intact glycopeptides by mass spectrometry allows information about glycan location and composition to be ascertained.					
33908003	0	59	theme	Anion	48:52	arg1	Exchange					54:61	Strong Anion Exchange	41:61	Strong Anion Exchange	41:61	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	2	60	theme	technologies	257:268	arg1	emergence					229:237	The emergence	225:237	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins	225:317	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	7	61	theme	proteins	1098:1105	arg1	digestion					1085:1093	enzymatic digestion	1075:1093	enzymatic digestion of proteins into peptides	1075:1119	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	2	62	theme	glycosylation	382:394	arg1	role					374:377	the role	370:377	the role of glycosylation in biology, disease states, and other areas of interest	370:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	4	63	theme	glycan	659:664	arg1	diversity					666:674	extensive glycan diversity	649:674	extensive glycan diversity	649:674	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	2	64	gly	glycosylation	382:394	arg1	disease					408:414	disease states	408:421	disease states	408:421	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	64	gly	glycosylation	382:394	arg1	interest					443:450	interest	443:450	interest	443:450	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	2	65	theme	glycoproteomic	242:255	arg1	technologies					257:268	glycoproteomic technologies	242:268	glycoproteomic technologies	242:268	The emergence of glycoproteomic technologies to identify and characterize glycans on proteins has the potential to enable a better understanding the role of glycosylation in biology, disease states, and other areas of interest.					
33908003	7	66	theme	enzymatic	1075:1083	arg1	digestion					1085:1093	enzymatic digestion	1075:1093	enzymatic digestion of proteins into peptides	1075:1119	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	1	67	theme	important	163:171	arg1	modification					211:222	a biologically important and complex protein posttranslational modification	148:222	a biologically important and complex protein posttranslational modification	148:222	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	1	67	theme	important	163:171	arg1	Glycosylation					131:143	Glycosylation	131:143	Glycosylation	131:143	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	8	68	theme	total	1337:1341	arg1	identifications					1367:1381	total and unique glycopeptide identifications	1337:1381	total and unique glycopeptide identifications which can be mapped back to proteins	1337:1418	Glycopeptide data generated using the SAX-ERLIC enrichment yields a high number of total and unique glycopeptide identifications which can be mapped back to proteins.					
33908003	4	69	theme	extensive	649:657	arg1	diversity					666:674	extensive glycan diversity	649:674	extensive glycan diversity	649:674	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	0	70	theme	Electrostatic	67:79	arg1	Repulsion					81:89	Electrostatic Repulsion	67:89	Electrostatic Repulsion	67:89	Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.					
33908003	3	71	theme	glycopeptides	491:503	arg1	analysis					472:479	the analysis	468:479	the analysis of intact glycopeptides by mass spectrometry	468:524	In particular, the analysis of intact glycopeptides by mass spectrometry allows information about glycan location and composition to be ascertained.					
33908003	4	72	theme	relative	736:743	arg1	mixture					728:734	a complex mixture	718:734	a complex mixture relative to nonglycosylated peptides	718:771	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	7	73	gly	glycopeptides	1181:1193	arg1	samples					1211:1217	biological samples	1200:1217	biological samples	1200:1217	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	7	73	gly	glycopeptides	1181:1193	arg2	glycopeptides					1181:1193	glycopeptides	1181:1193	glycopeptides from biological samples	1181:1217	Following enzymatic digestion of proteins into peptides, SAX-ERLIC is performed by solid phase extraction to enrich glycopeptides from biological samples with subsequent LC-MS/MS analysis.					
33908003	4	74	from	diversity	666:674	arg1	mixture					728:734	a complex mixture	718:734	a complex mixture relative to nonglycosylated peptides	718:771	However, such analysis is often complicated by extensive glycan diversity and the low abundance of glycopeptides in a complex mixture relative to nonglycosylated peptides.					
33908003	1	75	theme	complex	177:183	arg1	modification					211:222	a biologically important and complex protein posttranslational modification	148:222	a biologically important and complex protein posttranslational modification	148:222	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	1	75	theme	complex	177:183	arg1	Glycosylation					131:143	Glycosylation	131:143	Glycosylation	131:143	Glycosylation is a biologically important and complex protein posttranslational modification.					
33908003	6	76	theme	hydrophilic	1013:1023	arg1	SAX-ERLIC					1053:1061	SAX-ERLIC	1053:1061	SAX-ERLIC	1053:1061	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
33908003	6	76	theme	hydrophilic	1013:1023	arg1	chromatography					1037:1050	hydrophilic interaction chromatography	1013:1050	hydrophilic interaction chromatography (SAX-ERLIC)	1013:1062	In this chapter, we described a glycopeptide enrichment method combining strong anion exchange, electrostatic repulsion, and hydrophilic interaction chromatography (SAX-ERLIC).					
34822827	6	0	theme	LEO	914:916	arg1	release					896:902	The release	892:902	The release of EG and LEO from the coatings	892:934	The release of EG and LEO from the coatings are followed simple diffusion mechanism.					
34822827	7	1	theme	added	983:987	arg1	oils					999:1002	added essential oils	983:1002	added essential oils	983:1002	After added essential oils, the antibacterial and antioxidant properties of the composite coatings are significantly enhanced.					
34822827	2	2	theme	tape	389:392	arg1	method					402:407	tape casting method	389:407	tape casting method	389:407	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	6	3	from	coatings	927:934	arg1	release					896:902	The release	892:902	The release of EG and LEO from the coatings	892:934	The release of EG and LEO from the coatings are followed simple diffusion mechanism.					
34822827	8	4	theme	fillets	1153:1159	arg1	process					1124:1130	the preservation process	1107:1130	the preservation process of the rainbow trout fillets	1107:1159	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	9	5	theme	%	1303:1303	arg1	%					1310:1310	6.09%-15.63%	1299:1310	6.09%-15.63%	1299:1310	The β-sheet content is 6.09%-15.63% higher than that of control, indicating the coatings are helpful to maintain the order of myofibril protein.					
34822827	1	6	theme	Plant	192:196	arg1	oils					208:211	Plant essential oils	192:211	Plant essential oils	192:211	Plant essential oils lose their activity due to unstable chemical properties and volatility, and the coating can improve their stability by encapsulating.					
34822827	10	7	theme	enzyme	1484:1489	arg1	activity					1491:1498	antioxidant enzyme activity	1472:1498	antioxidant enzyme activity	1472:1498	The composite coatings slowed down the decrease of antioxidant enzyme activity, thus delay the protein oxidation.					
34822827	9	8	theme	myofibril	1402:1410	arg1	protein					1412:1418	myofibril protein	1402:1418	myofibril protein	1402:1418	The β-sheet content is 6.09%-15.63% higher than that of control, indicating the coatings are helpful to maintain the order of myofibril protein.					
34822827	0	9	theme	excellent	125:133	arg1	effect					146:151	their excellent protective effect	119:151	their excellent protective effect on myofibril protein of rainbow trout	119:189	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	6	10	theme	EG	907:908	arg1	release					896:902	The release	892:902	The release of EG and LEO from the coatings	892:934	The release of EG and LEO from the coatings are followed simple diffusion mechanism.					
34822827	10	11	theme	composite	1425:1433	arg1	coatings					1435:1442	The composite coatings	1421:1442	The composite coatings slowed down the decrease of antioxidant enzyme activity	1421:1498	The composite coatings slowed down the decrease of antioxidant enzyme activity, thus delay the protein oxidation.					
34822827	11	12	theme	food	1645:1648	arg1	preservation					1650:1661	food preservation	1645:1661	food preservation	1645:1661	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	9	13	theme	protein	1412:1418	arg1	order					1393:1397	the order	1389:1397	the order of myofibril protein	1389:1418	The β-sheet content is 6.09%-15.63% higher than that of control, indicating the coatings are helpful to maintain the order of myofibril protein.					
34822827	4	14	theme	composite	738:746	arg1	coatings					748:755	the composite coatings	734:755	the composite coatings	734:755	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	4	15	dep	Meanwhile	698:706	arg1	fillets					774:780	fillets	774:780	fillets myofibril protein	774:798	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	4	16	theme	rainbow	760:766	arg1	trout					768:772	rainbow trout	760:772	rainbow trout	760:772	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	2	17	theme	essential	501:509	arg1	materials					466:474	film-forming materials	453:474	film-forming materials	453:474	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	17	theme	essential	501:509	arg1	LEO					516:518	LEO	516:518	LEO	516:518	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	17	theme	essential	501:509	arg1	oil					511:513	laurel essential oil	494:513	laurel essential oil (LEO)	494:519	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	4	18	theme	myofibril	782:790	arg1	protein					792:798	myofibril protein	782:798	myofibril protein	782:798	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	5	19	theme	FG	880:881	arg1	coating					883:889	FG coating	880:889	FG coating	880:889	The mechanical properties of the FG/CS/FG coatings are better than FG coating.					
34822827	1	20	theme	essential	198:206	arg1	oils					208:211	Plant essential oils	192:211	Plant essential oils	192:211	Plant essential oils lose their activity due to unstable chemical properties and volatility, and the coating can improve their stability by encapsulating.					
34822827	0	21	theme	protective	135:144	arg1	effect					146:151	their excellent protective effect	119:151	their excellent protective effect on myofibril protein of rainbow trout	119:189	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	11	22	theme	food	1666:1669	arg1	packaging					1671:1679	food packaging	1666:1679	food packaging	1666:1679	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	10	23	theme	antioxidant	1472:1482	arg1	activity					1491:1498	antioxidant enzyme activity	1472:1498	antioxidant enzyme activity	1472:1498	The composite coatings slowed down the decrease of antioxidant enzyme activity, thus delay the protein oxidation.					
34822827	8	24	theme	trout	1147:1151	arg1	fillets					1153:1159	the rainbow trout fillets	1135:1159	the rainbow trout fillets	1135:1159	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	3	25	theme	composite	543:551	arg1	coatings					553:560	The composite coatings	539:560	The composite coatings	539:560	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	8	26	theme	preservation	1111:1122	arg1	process					1124:1130	the preservation process	1107:1130	the preservation process of the rainbow trout fillets	1107:1159	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	2	27	theme	flaxseed	414:421	arg1	gum					423:425	flaxseed gum	414:425	flaxseed gum (FG)	414:430	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	27	theme	flaxseed	414:421	arg1	FG					428:429	FG	428:429	FG	428:429	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	5	28	theme	mechanical	817:826	arg1	properties					828:837	The mechanical properties	813:837	The mechanical properties of the FG/CS/FG coatings	813:862	The mechanical properties of the FG/CS/FG coatings are better than FG coating.					
34822827	5	28	theme	mechanical	817:826	arg1	better					868:873	better	868:873	better	868:873	The mechanical properties of the FG/CS/FG coatings are better than FG coating.					
34822827	11	29	contain	have	1621:1624	arg2	value					1636:1640	potential value	1626:1640	potential value	1626:1640	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	11	29	contain	have	1621:1624	arg1	coatings					1612:1619	the composite coatings	1598:1619	the composite coatings	1598:1619	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	3	30	theme	antioxidant	658:668	arg1	properties					608:617	their physicochemical properties	586:617	their physicochemical properties	586:617	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	3	30	theme	antioxidant	658:668	arg1	properties					670:679	antibacterial and antioxidant properties	640:679	antibacterial and antioxidant properties	640:679	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	11	31	theme	long-term	1546:1554	arg1	properties					1586:1595	long-term antibacterial and antioxidant properties	1546:1595	long-term antibacterial and antioxidant properties	1546:1595	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	11	32	theme	antioxidant	1574:1584	arg1	properties					1586:1595	long-term antibacterial and antioxidant properties	1546:1595	long-term antibacterial and antioxidant properties	1546:1595	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	0	33	theme	composite	62:70	arg1	coatings					72:79	gum composite coatings	58:79	gum composite coatings	58:79	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	3	34	theme	antibacterial	640:652	arg1	properties					608:617	their physicochemical properties	586:617	their physicochemical properties	586:617	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	3	34	theme	antibacterial	640:652	arg1	properties					670:679	antibacterial and antioxidant properties	640:679	antibacterial and antioxidant properties	640:679	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	0	35	theme	flaxseed	27:34	arg1	Preparation					0:10	Preparation	0:10	Preparation of three-layer flaxseed	0:34	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	0	36	theme	myofibril	156:164	arg1	protein					166:172	myofibril protein	156:172	myofibril protein	156:172	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	11	37	theme	antibacterial	1556:1568	arg1	properties					1586:1595	long-term antibacterial and antioxidant properties	1546:1595	long-term antibacterial and antioxidant properties	1546:1595	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	0	38	theme	three-layer	15:25	arg1	flaxseed					27:34	three-layer flaxseed	15:34	three-layer flaxseed	15:34	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	6	39	theme	diffusion	956:964	arg1	mechanism					966:974	simple diffusion mechanism	949:974	simple diffusion mechanism	949:974	The release of EG and LEO from the coatings are followed simple diffusion mechanism.					
34822827	10	40	theme	activity	1491:1498	arg1	decrease					1460:1467	the decrease	1456:1467	the decrease of antioxidant enzyme activity	1456:1498	The composite coatings slowed down the decrease of antioxidant enzyme activity, thus delay the protein oxidation.					
34822827	4	41	theme	coatings	748:755	arg1	effect					724:729	the protective effect	709:729	the protective effect of the composite coatings on rainbow trout	709:772	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	5	42	theme	FG/CS/FG	846:853	arg1	coatings					855:862	the FG/CS/FG coatings	842:862	the FG/CS/FG coatings	842:862	The mechanical properties of the FG/CS/FG coatings are better than FG coating.					
34822827	0	43	theme	gum	58:60	arg1	coatings					72:79	gum composite coatings	58:79	gum composite coatings	58:79	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	6	44	theme	simple	949:954	arg1	mechanism					966:974	simple diffusion mechanism	949:974	simple diffusion mechanism	949:974	The release of EG and LEO from the coatings are followed simple diffusion mechanism.					
34822827	7	45	theme	composite	1057:1065	arg1	coatings					1067:1074	the composite coatings	1053:1074	the composite coatings	1053:1074	After added essential oils, the antibacterial and antioxidant properties of the composite coatings are significantly enhanced.					
34822827	0	46	from	properties	104:113	arg1	protein					166:172	myofibril protein	156:172	myofibril protein	156:172	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	10	47	theme	protein	1516:1522	arg1	oxidation					1524:1532	the protein oxidation	1512:1532	the protein oxidation	1512:1532	The composite coatings slowed down the decrease of antioxidant enzyme activity, thus delay the protein oxidation.					
34822827	8	48	theme	Ca2+-ATPase	1254:1264	arg1	activity					1266:1273	Ca2+-ATPase activity	1254:1273	Ca2+-ATPase activity	1254:1273	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	5	49	theme	coatings	855:862	arg1	properties					828:837	The mechanical properties	813:837	The mechanical properties of the FG/CS/FG coatings	813:862	The mechanical properties of the FG/CS/FG coatings are better than FG coating.					
34822827	5	49	theme	coatings	855:862	arg1	better					868:873	better	868:873	better	868:873	The mechanical properties of the FG/CS/FG coatings are better than FG coating.					
34822827	7	50	theme	coatings	1067:1074	arg1	properties					1039:1048	the antibacterial and antioxidant properties	1005:1048	the antibacterial and antioxidant properties of the composite coatings	1005:1074	After added essential oils, the antibacterial and antioxidant properties of the composite coatings are significantly enhanced.					
34822827	3	51	theme	release	620:626	arg1	properties					608:617	their physicochemical properties	586:617	their physicochemical properties	586:617	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	3	51	theme	release	620:626	arg1	properties					628:637	release properties	620:637	release properties	620:637	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	7	52	theme	antibacterial	1009:1021	arg1	properties					1039:1048	the antibacterial and antioxidant properties	1005:1048	the antibacterial and antioxidant properties of the composite coatings	1005:1074	After added essential oils, the antibacterial and antioxidant properties of the composite coatings are significantly enhanced.					
34822827	2	53	theme	laurel	494:499	arg1	materials					466:474	film-forming materials	453:474	film-forming materials	453:474	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	53	theme	laurel	494:499	arg1	LEO					516:518	LEO	516:518	LEO	516:518	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	53	theme	laurel	494:499	arg1	oil					511:513	laurel essential oil	494:513	laurel essential oil (LEO)	494:519	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	8	54	theme	sulfhydryl	1231:1240	arg1	content					1242:1248	the sulfhydryl content	1227:1248	the sulfhydryl content	1227:1248	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	8	55	theme	composite	1166:1174	arg1	coatings					1176:1183	the composite coatings	1162:1183	the composite coatings	1162:1183	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	0	56	theme	trout	185:189	arg1	properties					104:113	sustained-release properties	86:113	sustained-release properties	86:113	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	0	56	theme	trout	185:189	arg1	effect					146:151	their excellent protective effect	119:151	their excellent protective effect on myofibril protein of rainbow trout	119:189	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	11	57	theme	composite	1602:1610	arg1	coatings					1612:1619	the composite coatings	1598:1619	the composite coatings	1598:1619	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	4	58	theme	protective	713:722	arg1	effect					724:729	the protective effect	709:729	the protective effect of the composite coatings on rainbow trout	709:772	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	2	59	theme	film-forming	453:464	arg1	materials					466:474	film-forming materials	453:474	film-forming materials	453:474	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	59	theme	film-forming	453:464	arg1	oil					511:513	laurel essential oil	494:513	laurel essential oil (LEO)	494:519	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	59	theme	film-forming	453:464	arg1	coatings					363:370	The three-layer coatings	347:370	The three-layer coatings	347:370	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	59	theme	film-forming	453:464	arg1	eugenol					477:483	eugenol	477:483	eugenol (EG)	477:488	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	11	60	dep	preservation	1650:1661	arg1	materials					1681:1689	materials	1681:1689	materials	1681:1689	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	0	61	theme	rainbow	177:183	arg1	trout					185:189	rainbow trout	177:189	rainbow trout	177:189	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	0	62	from	effect	146:151	arg1	protein					166:172	myofibril protein	156:172	myofibril protein	156:172	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	7	63	theme	antioxidant	1027:1037	arg1	properties					1039:1048	the antibacterial and antioxidant properties	1005:1048	the antibacterial and antioxidant properties of the composite coatings	1005:1074	After added essential oils, the antibacterial and antioxidant properties of the composite coatings are significantly enhanced.					
34822827	8	64	theme	carbonyl	1200:1207	arg1	content					1209:1215	the carbonyl content	1196:1215	the carbonyl content	1196:1215	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	4	65	from	effect	724:729	arg1	trout					768:772	rainbow trout	760:772	rainbow trout	760:772	Meanwhile, the protective effect of the composite coatings on rainbow trout fillets myofibril protein was studied.					
34822827	1	66	theme	chemical	249:256	arg1	properties					258:267	unstable chemical properties	240:267	unstable chemical properties	240:267	Plant essential oils lose their activity due to unstable chemical properties and volatility, and the coating can improve their stability by encapsulating.					
34822827	8	67	dep	reduce	1189:1194	arg1	increase					1218:1225	increase	1218:1225	increase the sulfhydryl content and Ca2+-ATPase activity	1218:1273	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	9	68	theme	β-sheet	1280:1286	arg1	higher					1312:1317	higher	1312:1317	higher	1312:1317	The β-sheet content is 6.09%-15.63% higher than that of control, indicating the coatings are helpful to maintain the order of myofibril protein.					
34822827	9	68	theme	β-sheet	1280:1286	arg1	content					1288:1294	The β-sheet content	1276:1294	The β-sheet content	1276:1294	The β-sheet content is 6.09%-15.63% higher than that of control, indicating the coatings are helpful to maintain the order of myofibril protein.					
34822827	7	69	theme	essential	989:997	arg1	oils					999:1002	added essential oils	983:1002	added essential oils	983:1002	After added essential oils, the antibacterial and antioxidant properties of the composite coatings are significantly enhanced.					
34822827	2	70	theme	three-layer	351:361	arg1	materials					466:474	film-forming materials	453:474	film-forming materials	453:474	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	70	theme	three-layer	351:361	arg1	preservatives					524:536	preservatives	524:536	preservatives	524:536	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	2	70	theme	three-layer	351:361	arg1	coatings					363:370	The three-layer coatings	347:370	The three-layer coatings	347:370	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34822827	0	71	theme	sustained-release	86:102	arg1	properties					104:113	sustained-release properties	86:113	sustained-release properties	86:113	Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout.					
34822827	1	72	theme	unstable	240:247	arg1	properties					258:267	unstable chemical properties	240:267	unstable chemical properties	240:267	Plant essential oils lose their activity due to unstable chemical properties and volatility, and the coating can improve their stability by encapsulating.					
34822827	11	73	theme	potential	1626:1634	arg1	value					1636:1640	potential value	1626:1640	potential value	1626:1640	Because of long-term antibacterial and antioxidant properties, the composite coatings have potential value in food preservation or food packaging materials.					
34822827	8	74	theme	rainbow	1139:1145	arg1	trout					1147:1151	rainbow trout	1139:1151	the rainbow trout fillets	1135:1159	In the preservation process of the rainbow trout fillets, the composite coatings can reduce the carbonyl content, increase the sulfhydryl content and Ca2+-ATPase activity.					
34822827	3	75	theme	physicochemical	592:606	arg1	properties					670:679	antibacterial and antioxidant properties	640:679	antibacterial and antioxidant properties	640:679	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	3	75	theme	physicochemical	592:606	arg1	properties					608:617	their physicochemical properties	586:617	their physicochemical properties	586:617	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	3	75	theme	physicochemical	592:606	arg1	properties					628:637	release properties	620:637	release properties	620:637	The composite coatings were characterized, and their physicochemical properties, release properties, antibacterial and antioxidant properties were determined.					
34822827	2	76	theme	casting	394:400	arg1	method					402:407	tape casting method	389:407	tape casting method	389:407	The three-layer coatings were prepared by tape casting method with flaxseed gum (FG) and chitosan (CS) as film-forming materials, eugenol (EG) and laurel essential oil (LEO) as preservatives.					
34364635	0	0	theme	pressure	98:105	arg1	sensing					107:113	pressure sensing	98:113	pressure sensing	98:113	Thermal insulating, light-weight and conductive cellulose/aramid nanofibers composite aerogel for pressure sensing.					
34364635	7	1	theme	electrochemical	1050:1064	arg1	performance					1066:1076	satisfactory electrochemical performance	1037:1076	satisfactory electrochemical performance	1037:1076	Moreover, the aerogel sensor revealed high strain sensitivity and satisfactory electrochemical performance.					
34364635	1	2	theme	pressure	190:197	arg1	sensing					199:205	pressure sensing	190:205	pressure sensing	190:205	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	8	3	theme	controllable	1108:1119	arg1	alignment					1121:1129	controllable alignment	1108:1129	controllable alignment	1108:1129	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	8	4	from	sensor	1211:1216	arg1	promising					1189:1197	promising	1189:1197	promising	1189:1197	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	8	5	theme	Lightweight	1079:1089	arg1	aerogel					1095:1101	Lightweight CAP aerogel	1079:1101	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability	1079:1179	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	8	6	theme	sensitive	1132:1140	arg1	property					1150:1157	sensitive sensing property	1132:1157	sensitive sensing property	1132:1157	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	7	7	theme	high	1009:1012	arg1	sensitivity					1021:1031	high strain sensitivity	1009:1031	high strain sensitivity	1009:1031	Moreover, the aerogel sensor revealed high strain sensitivity and satisfactory electrochemical performance.					
34364635	2	8	theme	mechanical	331:340	arg1	properties					342:351	weak mechanical properties	326:351	weak mechanical properties	326:351	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF) was designed as reinforcement to overcome the shortcoming mentioned above.					
34364635	8	9	with	aerogel	1095:1101	arg1	property					1150:1157	sensitive sensing property	1132:1157	sensitive sensing property	1132:1157	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	8	9	with	aerogel	1095:1101	arg1	stability					1171:1179	thermal stability	1163:1179	thermal stability	1163:1179	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	8	9	with	aerogel	1095:1101	arg1	alignment					1121:1129	controllable alignment	1108:1129	controllable alignment	1108:1129	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	6	10	theme	destruction	947:957	arg1	phenomenon					959:968	any destruction phenomenon	943:968	any destruction phenomenon	943:968	And it could withstand high temperature without any destruction phenomenon.					
34364635	2	11	theme	weak	326:329	arg1	properties					342:351	weak mechanical properties	326:351	weak mechanical properties	326:351	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF) was designed as reinforcement to overcome the shortcoming mentioned above.					
34364635	1	12	from	attention	177:185	arg1	sensing					199:205	pressure sensing	190:205	pressure sensing	190:205	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	3	13	theme	CA	559:560	arg1	aerogel					563:569	nanocellulose/aramid nanofiber (CA) aerogel	527:569	nanocellulose/aramid nanofiber (CA) aerogel	527:569	Herein, the unidirectional freeze casting method was proposed to fabricate nanocellulose/aramid nanofiber (CA) aerogel.					
34364635	3	14	theme	nanocellulose/aramid	527:546	arg1	aerogel					563:569	nanocellulose/aramid nanofiber (CA) aerogel	527:569	nanocellulose/aramid nanofiber (CA) aerogel	527:569	Herein, the unidirectional freeze casting method was proposed to fabricate nanocellulose/aramid nanofiber (CA) aerogel.					
34364635	7	15	theme	strain	1014:1019	arg1	sensitivity					1021:1031	high strain sensitivity	1009:1031	high strain sensitivity	1009:1031	Moreover, the aerogel sensor revealed high strain sensitivity and satisfactory electrochemical performance.					
34364635	2	16	dep	Limited	294:300	arg1	aramid					354:359	aramid	354:359	aramid	354:359	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF) was designed as reinforcement to overcome the shortcoming mentioned above.					
34364635	5	17	theme	ordered	798:804	arg1	microstructure					806:819	the ordered microstructure	794:819	the ordered microstructure	794:819	The conductive aerogel with the ordered microstructure exhibited the anisotropic mechanical properties and thermal conductivity.					
34364635	8	18	theme	CAP	1091:1093	arg1	aerogel					1095:1101	Lightweight CAP aerogel	1079:1101	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability	1079:1179	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	5	19	theme	mechanical	847:856	arg1	properties					858:867	the anisotropic mechanical properties	831:867	the anisotropic mechanical properties	831:867	The conductive aerogel with the ordered microstructure exhibited the anisotropic mechanical properties and thermal conductivity.					
34364635	1	20	theme	wearable	211:218	arg1	devices					220:226	wearable devices	211:226	wearable devices owing to lightweight, sustainability and good chemical stability	211:291	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	5	21	theme	anisotropic	835:845	arg1	properties					858:867	the anisotropic mechanical properties	831:867	the anisotropic mechanical properties	831:867	The conductive aerogel with the ordered microstructure exhibited the anisotropic mechanical properties and thermal conductivity.					
34364635	8	22	theme	thermal	1163:1169	arg1	stability					1171:1179	thermal stability	1163:1179	thermal stability	1163:1179	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	8	23	theme	extreme	1229:1235	arg1	conditions					1237:1246	some extreme conditions	1224:1246	some extreme conditions	1224:1246	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	4	24	theme	conductive	689:698	arg1	PPy					713:715	PPy	713:715	PPy	713:715	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	4	24	theme	conductive	689:698	arg1	polypyrrole					700:710	conductive polypyrrole	689:710	conductive polypyrrole (PPy)	689:716	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	0	25	theme	Thermal	0:6	arg1	insulating					8:17	Thermal insulating	0:17	Thermal insulating	0:17	Thermal insulating, light-weight and conductive cellulose/aramid nanofibers composite aerogel for pressure sensing.					
34364635	4	26	theme	CAP	590:592	arg1	aerogel					595:601	the CA/PPy (CAP) aerogel	578:601	the CA/PPy (CAP) aerogel	578:601	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	4	27	theme	composite	747:755	arg1	aerogel					757:763	the composite aerogel	743:763	the composite aerogel	743:763	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	3	28	theme	unidirectional	464:477	arg1	method					494:499	the unidirectional freeze casting method	460:499	the unidirectional freeze casting method	460:499	Herein, the unidirectional freeze casting method was proposed to fabricate nanocellulose/aramid nanofiber (CA) aerogel.					
34364635	7	29	theme	aerogel	985:991	arg1	sensor					993:998	the aerogel sensor	981:998	the aerogel sensor	981:998	Moreover, the aerogel sensor revealed high strain sensitivity and satisfactory electrochemical performance.					
34364635	1	30	theme	Conductive	116:125	arg1	aerogels					141:148	Conductive nanocellulose aerogels	116:148	Conductive nanocellulose aerogels	116:148	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	4	31	theme	aerogel	654:660	arg1	structure					638:646	the oriented structure	625:646	the oriented structure of CA aerogel	625:660	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	4	31	theme	aerogel	654:660	arg1	template					667:674	a template	665:674	a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel	665:763	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	6	32	theme	high	918:921	arg1	temperature					923:933	high temperature	918:933	high temperature	918:933	And it could withstand high temperature without any destruction phenomenon.					
34364635	1	33	theme	nanocellulose	127:139	arg1	aerogels					141:148	Conductive nanocellulose aerogels	116:148	Conductive nanocellulose aerogels	116:148	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	4	34	theme	CA	651:652	arg1	aerogel					654:660	CA aerogel	651:660	CA aerogel	651:660	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	0	35	theme	conductive	37:46	arg1	cellulose/aramid					48:63	conductive cellulose/aramid	37:63	conductive cellulose/aramid	37:63	Thermal insulating, light-weight and conductive cellulose/aramid nanofibers composite aerogel for pressure sensing.					
34364635	3	36	theme	freeze	479:484	arg1	method					494:499	the unidirectional freeze casting method	460:499	the unidirectional freeze casting method	460:499	Herein, the unidirectional freeze casting method was proposed to fabricate nanocellulose/aramid nanofiber (CA) aerogel.					
34364635	8	37	theme	pressure	1202:1209	arg1	sensor					1211:1216	pressure sensor	1202:1216	pressure sensor under some extreme conditions	1202:1246	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	3	38	theme	nanofiber	548:556	arg1	aerogel					563:569	nanocellulose/aramid nanofiber (CA) aerogel	527:569	nanocellulose/aramid nanofiber (CA) aerogel	527:569	Herein, the unidirectional freeze casting method was proposed to fabricate nanocellulose/aramid nanofiber (CA) aerogel.					
34364635	4	39	theme	CA/PPy	582:587	arg1	aerogel					595:601	the CA/PPy (CAP) aerogel	578:601	the CA/PPy (CAP) aerogel	578:601	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	8	40	theme	sensing	1142:1148	arg1	property					1150:1157	sensitive sensing property	1132:1157	sensitive sensing property	1132:1157	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
34364635	4	41	theme	in-situ	718:724	arg1	formation					726:734	in-situ formation	718:734	in-situ formation	718:734	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	4	42	theme	oriented	629:636	arg1	structure					638:646	the oriented structure	625:646	the oriented structure of CA aerogel	625:660	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	4	42	theme	oriented	629:636	arg1	template					667:674	a template	665:674	a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel	665:763	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	4	43	dep	inducing	680:687	arg1	inside					736:741	inside	736:741	inside the composite aerogel	736:763	Then, the CA/PPy (CAP) aerogel was obtained by using the oriented structure of CA aerogel as a template for inducing conductive polypyrrole (PPy) in-situ formation inside the composite aerogel.					
34364635	1	44	theme	significant	165:175	arg1	attention					177:185	significant attention	165:185	significant attention in pressure sensing	165:205	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	1	45	theme	good	269:272	arg1	lightweight					237:247	lightweight	237:247	lightweight	237:247	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	1	45	theme	good	269:272	arg1	stability					283:291	good chemical stability	269:291	good chemical stability	269:291	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	5	46	theme	conductive	770:779	arg1	aerogel					781:787	The conductive aerogel	766:787	The conductive aerogel with the ordered microstructure	766:819	The conductive aerogel with the ordered microstructure exhibited the anisotropic mechanical properties and thermal conductivity.					
34364635	2	47	theme	Limited	294:300	arg1	reinforcement					393:405	reinforcement	393:405	reinforcement	393:405	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF) was designed as reinforcement to overcome the shortcoming mentioned above.					
34364635	2	47	theme	Limited	294:300	arg1	ANF					372:374	ANF	372:374	ANF	372:374	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF) was designed as reinforcement to overcome the shortcoming mentioned above.					
34364635	2	47	theme	Limited	294:300	arg1	nanofiber					361:369	Limited by its flammability and weak mechanical properties, aramid nanofiber	294:369	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF)	294:375	Limited by its flammability and weak mechanical properties, aramid nanofiber (ANF) was designed as reinforcement to overcome the shortcoming mentioned above.					
34364635	5	48	theme	thermal	873:879	arg1	conductivity					881:892	thermal conductivity	873:892	thermal conductivity	873:892	The conductive aerogel with the ordered microstructure exhibited the anisotropic mechanical properties and thermal conductivity.					
34364635	3	49	theme	casting	486:492	arg1	method					494:499	the unidirectional freeze casting method	460:499	the unidirectional freeze casting method	460:499	Herein, the unidirectional freeze casting method was proposed to fabricate nanocellulose/aramid nanofiber (CA) aerogel.					
34364635	1	50	theme	chemical	274:281	arg1	lightweight					237:247	lightweight	237:247	lightweight	237:247	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	1	50	theme	chemical	274:281	arg1	stability					283:291	good chemical stability	269:291	good chemical stability	269:291	Conductive nanocellulose aerogels have attracted significant attention in pressure sensing for wearable devices owing to lightweight, sustainability and good chemical stability.					
34364635	0	51	theme	composite	76:84	arg1	aerogel					86:92	composite aerogel	76:92	composite aerogel for pressure sensing	76:113	Thermal insulating, light-weight and conductive cellulose/aramid nanofibers composite aerogel for pressure sensing.					
34364635	5	52	with	aerogel	781:787	arg1	microstructure					806:819	the ordered microstructure	794:819	the ordered microstructure	794:819	The conductive aerogel with the ordered microstructure exhibited the anisotropic mechanical properties and thermal conductivity.					
34364635	7	53	theme	satisfactory	1037:1048	arg1	performance					1066:1076	satisfactory electrochemical performance	1037:1076	satisfactory electrochemical performance	1037:1076	Moreover, the aerogel sensor revealed high strain sensitivity and satisfactory electrochemical performance.					
34364635	8	54	from	promising	1189:1197	arg1	sensor					1211:1216	pressure sensor	1202:1216	pressure sensor under some extreme conditions	1202:1246	Lightweight CAP aerogel with controllable alignment, sensitive sensing property and thermal stability is very promising in pressure sensor under some extreme conditions.					
32146511	5	0	theme	citric	580:585	arg1	acid					587:590	citric acid	580:590	citric acid	580:590	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	4	1	theme	FTIR	470:473	arg1	techniques					486:495	FTIR analytical techniques	470:495	FTIR analytical techniques	470:495	The prepared samples were characterized using XRD, SEM, TGA and FTIR analytical techniques.					
32146511	8	2	theme	composite	1020:1028	arg1	nature					979:984	the non-cytotoxic nature	961:984	the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite	961:1028	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	6	3	theme	stronger	708:715	arg1	capability					717:726	stronger capability	708:726	stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times)	708:796	The results of the catalytic assay showed that the ZnO2/CA-βCD composite displayed stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times).					
32146511	1	4	theme	H2O2	147:150	arg1	activity					163:170	the H2O2 scavenging activity	143:170	the H2O2 scavenging activity of the ZnO2/β-CD	143:187	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	1	4	theme	H2O2	147:150	arg1	composite					208:216	a biocompatible composite	192:216	a biocompatible composite	192:216	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	8	5	theme	ZnO2/CA-βCD	1008:1018	arg1	composite					1020:1028	ZnO2/CA-βCD composite	1008:1028	ZnO2/CA-βCD composite	1008:1028	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	1	6	theme	scavenging	152:161	arg1	activity					163:170	the H2O2 scavenging activity	143:170	the H2O2 scavenging activity of the ZnO2/β-CD	143:187	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	1	6	theme	scavenging	152:161	arg1	composite					208:216	a biocompatible composite	192:216	a biocompatible composite	192:216	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	5	7	theme	acid	587:590	arg1	presence					568:575	the presence	564:575	the presence of citric acid and β-cyclodextrin in composite	564:622	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	8	8	theme	assay	937:941	arg1	studies					943:949	MTT assay studies	933:949	MTT assay studies	933:949	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	8	9	theme	MTT	933:935	arg1	studies					943:949	MTT assay studies	933:949	MTT assay studies	933:949	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	0	10	link	acid-crosslinked	7:22	arg1	β-cyclodextrin					24:37	Citric acid-crosslinked β-cyclodextrin	0:37	Citric acid-crosslinked β-cyclodextrin	0:37	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	5	11	theme	β-cyclodextrin	596:609	arg1	presence					568:575	the presence	564:575	the presence of citric acid and β-cyclodextrin in composite	564:622	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	8	12	theme	non-cytotoxic	965:977	arg1	nature					979:984	the non-cytotoxic nature	961:984	the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite	961:1028	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	8	13	theme	ZnO2	989:992	arg1	particles					994:1002	ZnO2 particles	989:1002	ZnO2 particles	989:1002	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	6	14	theme	ZnO2/CA-βCD	676:686	arg1	composite					688:696	the ZnO2/CA-βCD composite	672:696	the ZnO2/CA-βCD composite	672:696	The results of the catalytic assay showed that the ZnO2/CA-βCD composite displayed stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times).					
32146511	0	15	theme	acid-crosslinked	7:22	arg1	β-cyclodextrin					24:37	Citric acid-crosslinked β-cyclodextrin	0:37	Citric acid-crosslinked β-cyclodextrin	0:37	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	7	16	theme	β-cyclodextrin	917:930	arg1	presence					889:896	the presence	885:896	the presence of citric acid and β-cyclodextrin	885:930	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
32146511	7	17	theme	adsorption	830:839	arg1	capacity					841:848	increased adsorption capacity	820:848	increased adsorption capacity	820:848	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
32146511	0	18	theme	Citric	0:5	arg1	β-cyclodextrin					24:37	Citric acid-crosslinked β-cyclodextrin	0:37	Citric acid-crosslinked β-cyclodextrin	0:37	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	7	19	theme	citric	901:906	arg1	acid					908:911	citric acid	901:911	citric acid	901:911	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
32146511	1	20	theme	ZnO2/β-CD	179:187	arg1	activity					163:170	the H2O2 scavenging activity	143:170	the H2O2 scavenging activity of the ZnO2/β-CD	143:187	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	1	20	theme	ZnO2/β-CD	179:187	arg1	composite					208:216	a biocompatible composite	192:216	a biocompatible composite	192:216	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	6	21	theme	ZnO2	763:766	arg1	times					791:795	about seven times	779:795	about seven times	779:795	The results of the catalytic assay showed that the ZnO2/CA-βCD composite displayed stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times).					
32146511	6	21	theme	ZnO2	763:766	arg1	particles					768:776	ZnO2 particles	763:776	ZnO2 particles (about seven times)	763:796	The results of the catalytic assay showed that the ZnO2/CA-βCD composite displayed stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times).					
32146511	6	22	theme	assay	654:658	arg1	results					629:635	The results	625:635	The results of the catalytic assay	625:658	The results of the catalytic assay showed that the ZnO2/CA-βCD composite displayed stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times).					
32146511	3	23	mod	modified	325:332	arg3	acid					344:347	citric acid	337:347	citric acid (CA)	337:352	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	3	23	mod	modified	325:332	arg3	CA					350:351	CA	350:351	CA	350:351	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	3	23	mod	modified	325:332	arg1	β-cyclodextrin					306:319	β-cyclodextrin	306:319	β-cyclodextrin	306:319	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	5	24	from	presence	568:575	arg1	composite					614:622	composite	614:622	composite	614:622	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	6	25	theme	catalytic	644:652	arg1	assay					654:658	the catalytic assay	640:658	the catalytic assay	640:658	The results of the catalytic assay showed that the ZnO2/CA-βCD composite displayed stronger capability to decompose H2O2 in comparison to ZnO2 particles (about seven times).					
32146511	1	26	theme	biocompatible	194:206	arg1	activity					163:170	the H2O2 scavenging activity	143:170	the H2O2 scavenging activity of the ZnO2/β-CD	143:187	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	1	26	theme	biocompatible	194:206	arg1	composite					208:216	a biocompatible composite	192:216	a biocompatible composite	192:216	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	0	27	theme	zinc	49:52	arg1	scavenger					87:95	a biocompatible H2O2 scavenger	66:95	a biocompatible H2O2 scavenger	66:95	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	0	27	theme	zinc	49:52	arg1	peroxide					54:61	zinc peroxide	49:61	zinc peroxide	49:61	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	8	28	theme	particles	994:1002	arg1	nature					979:984	the non-cytotoxic nature	961:984	the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite	961:1028	MTT assay studies confirmed the non-cytotoxic nature of ZnO2 particles and ZnO2/CA-βCD composite.					
32146511	5	29	theme	typical	523:529	arg1	pattern					531:537	a typical pattern	521:537	a typical pattern of ZnO2	521:545	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	5	30	attach	presence	568:575	arg1	composite					614:622	composite	614:622	composite	614:622	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	5	30	attach	presence	568:575	arg2	β-cyclodextrin					596:609	β-cyclodextrin	596:609	β-cyclodextrin	596:609	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	5	30	attach	presence	568:575	arg2	acid					587:590	citric acid	580:590	citric acid	580:590	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	3	31	theme	citric	337:342	arg1	CA					350:351	CA	350:351	CA	350:351	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	3	31	theme	citric	337:342	arg1	acid					344:347	citric acid	337:347	citric acid (CA)	337:352	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	2	32	theme	Zinc	219:222	arg1	peroxide					224:231	Zinc peroxide	219:231	Zinc peroxide	219:231	Zinc peroxide was prepared via sonochemical approach.					
32146511	1	33	theme	present	113:119	arg1	study					121:125	the present study	109:125	the present study	109:125	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	3	34	theme	ultrasonic	382:391	arg1	irradiation					393:403	ultrasonic irradiation	382:403	ultrasonic irradiation	382:403	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	4	35	theme	prepared	410:417	arg1	samples					419:425	The prepared samples	406:425	The prepared samples	406:425	The prepared samples were characterized using XRD, SEM, TGA and FTIR analytical techniques.					
32146511	1	36	theme	study	121:125	arg1	aim					102:104	The aim	98:104	The aim of the present study	98:125	The aim of the present study was to evaluate the H2O2 scavenging activity of the ZnO2/β-CD as a biocompatible composite.					
32146511	7	37	theme	acid	908:911	arg1	presence					889:896	the presence	885:896	the presence of citric acid and β-cyclodextrin	885:930	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
32146511	3	38	theme	green	289:293	arg1	composite					295:303	a green composite	287:303	a green composite	287:303	To synthesize a green composite, β-cyclodextrin was modified by citric acid (CA) and reacted with ZnO2 under ultrasonic irradiation.					
32146511	5	39	theme	ZnO2	542:545	arg1	pattern					531:537	a typical pattern	521:537	a typical pattern of ZnO2	521:545	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	2	40	theme	sonochemical	250:261	arg1	approach					263:270	sonochemical approach	250:270	sonochemical approach	250:270	Zinc peroxide was prepared via sonochemical approach.					
32146511	5	41	theme	XRD	498:500	arg1	analysis					502:509	XRD analysis	498:509	XRD analysis	498:509	XRD analysis exhibited a typical pattern of ZnO2 and demonstrated the presence of citric acid and β-cyclodextrin in composite.					
32146511	7	42	theme	increased	820:828	arg1	capacity					841:848	increased adsorption capacity	820:848	increased adsorption capacity	820:848	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
32146511	0	43	theme	H2O2	82:85	arg1	scavenger					87:95	a biocompatible H2O2 scavenger	66:95	a biocompatible H2O2 scavenger	66:95	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	0	43	theme	H2O2	82:85	arg1	peroxide					54:61	zinc peroxide	49:61	zinc peroxide	49:61	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	0	44	theme	biocompatible	68:80	arg1	scavenger					87:95	a biocompatible H2O2 scavenger	66:95	a biocompatible H2O2 scavenger	66:95	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	0	44	theme	biocompatible	68:80	arg1	peroxide					54:61	zinc peroxide	49:61	zinc peroxide	49:61	Citric acid-crosslinked β-cyclodextrin supported zinc peroxide as a biocompatible H2O2 scavenger.					
32146511	4	45	theme	analytical	475:484	arg1	techniques					486:495	FTIR analytical techniques	470:495	FTIR analytical techniques	470:495	The prepared samples were characterized using XRD, SEM, TGA and FTIR analytical techniques.					
32146511	7	46	theme	composite	868:876	arg1	solubility					854:863	solubility	854:863	solubility	854:863	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
32146511	7	46	theme	composite	868:876	arg1	capacity					841:848	increased adsorption capacity	820:848	increased adsorption capacity	820:848	It was attributed to increased adsorption capacity and solubility of composite due to the presence of citric acid and β-cyclodextrin.					
31945444	5	0	from	evident	956:962	arg1	properties					978:987	mechanical properties	967:987	mechanical properties	967:987	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	7	1	theme	thermal	1165:1171	arg1	resistance					1173:1182	the thermal resistance	1161:1182	the thermal resistance of all films	1161:1195	Crosslinking increased the thermal resistance of all films.					
31945444	10	2	theme	visible	1430:1436	arg1	region					1438:1443	the visible region	1426:1443	the visible region	1426:1443	The films exhibited high transmittance in the visible region.					
31945444	7	3	theme	films	1191:1195	arg1	resistance					1173:1182	the thermal resistance	1161:1182	the thermal resistance of all films	1161:1195	Crosslinking increased the thermal resistance of all films.					
31945444	4	4	theme	alginate	713:720	arg1	films					722:726	crosslinked and plasticized alginate films	685:726	crosslinked and plasticized alginate films	685:726	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	0	5	theme	glycerol	67:74	arg1	effect					32:37	the effect	28:37	the effect of calcium crosslinking and glycerol	28:74	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	8	6	link	crosslinked	1245:1255	arg1	films					1270:1274	highly crosslinked low swelling films	1238:1274	highly crosslinked low swelling films	1238:1274	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	2	7	from	%	291:291	arg1	glycerol/alginate					298:314	glycerol/alginate	298:314	glycerol/alginate	298:314	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	7	from	%	291:291	arg1	films					272:276	glycerol added alginate films	248:276	glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate)	248:315	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	4	8	theme	other	802:806	arg1	constant					808:815	the other constant	798:815	the other constant	798:815	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	5	9	theme	crosslinking	936:947	arg1	effect					926:931	the effect	922:931	the effect of crosslinking	922:947	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	4	10	theme	plasticized	701:711	arg1	films					722:726	crosslinked and plasticized alginate films	685:726	crosslinked and plasticized alginate films	685:726	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	3	11	from	variation	476:484	arg1	compositions					561:572	twenty different compositions	544:572	twenty different compositions	544:572	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	6	12	theme	strain	1119:1124	arg1	properties					1126:1135	fracture strain properties	1110:1135	fracture strain properties	1110:1135	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	5	13	theme	glycerol	903:910	arg1	addition					912:919	glycerol addition	903:919	glycerol addition	903:919	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	8	14	theme	low	1257:1259	arg1	films					1270:1274	highly crosslinked low swelling films	1238:1274	highly crosslinked low swelling films	1238:1274	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	6	15	theme	optimum	1066:1072	arg1	combinations					1074:1085	optimum combinations	1066:1085	optimum combinations with good strength and fracture strain properties	1066:1135	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	2	16	theme	chloride	391:398	arg1	solutions					400:408	calcium chloride solutions	383:408	calcium chloride solutions (0.5, 1, 1.5, 2%, w/w)	383:431	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	17	theme	films	272:276	arg1	spectrum					236:243	a wide spectrum	229:243	a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate)	229:315	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	8	18	theme	crosslinked	1202:1212	arg1	films					1228:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	6	19	theme	glycerol	1002:1009	arg1	concentrations					1011:1024	Calcium and glycerol concentrations	990:1024	concentrations	1011:1024	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	0	20	theme	physical	96:103	arg1	properties					105:114	the physical properties	92:114	the physical properties of alginate films	92:132	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	10	21	from	transmittance	1409:1421	arg1	region					1438:1443	the visible region	1426:1443	the visible region	1426:1443	The films exhibited high transmittance in the visible region.					
31945444	8	22	theme	crosslinked	1245:1255	arg1	films					1270:1274	highly crosslinked low swelling films	1238:1274	highly crosslinked low swelling films	1238:1274	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	2	23	theme	alginate	263:270	arg1	glycerol/alginate					298:314	glycerol/alginate	298:314	glycerol/alginate	298:314	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	23	theme	alginate	263:270	arg1	films					272:276	glycerol added alginate films	248:276	glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate)	248:315	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	24	theme	different	355:363	arg1	concentrations					365:378	four different concentrations	350:378	four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w)	350:431	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	0	25	theme	detailed	2:9	arg1	investigation					11:23	A detailed investigation	0:23	A detailed investigation of the effect of calcium crosslinking and glycerol	0:74	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	10	26	theme	high	1404:1407	arg1	transmittance					1409:1421	high transmittance	1404:1421	high transmittance in the visible region	1404:1443	The films exhibited high transmittance in the visible region.					
31945444	4	27	theme	these	783:787	arg1	these					783:787	these	783:787	these	783:787	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	4	27	theme	these	783:787	arg1	one					776:778	one	776:778	one	776:778	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	5	28	theme	negligible	855:864	arg1	effect					866:871	a negligible effect	853:871	a negligible effect	853:871	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	6	29	theme	Calcium	990:996	arg1	concentrations					1011:1024	Calcium and glycerol concentrations	990:1024	concentrations	1011:1024	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	9	30	theme	Water	1291:1295	arg1	permeability					1303:1314	Water vapor permeability	1291:1314	Water vapor permeability of films	1291:1323	Water vapor permeability of films decreased regularly with increasing calcium concentration.					
31945444	11	31	theme	food	1537:1540	arg1	applications					1552:1563	food packaging applications	1537:1563	food packaging applications	1537:1563	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
31945444	3	32	theme	plasticizer	498:508	arg1	concentrations					526:539	both the plasticizer and crosslinker concentrations	489:539	concentrations	526:539	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	8	33	theme	high	1214:1217	arg1	films					1228:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	1	34	theme	biocompatible	183:195	arg1	nature					215:220	their biocompatible and biodegradable nature	177:220	their biocompatible and biodegradable nature	177:220	Alginates attract growing interest due to their biocompatible and biodegradable nature.					
31945444	9	35	theme	vapor	1297:1301	arg1	permeability					1303:1314	Water vapor permeability	1291:1314	Water vapor permeability of films	1291:1323	Water vapor permeability of films decreased regularly with increasing calcium concentration.					
31945444	0	36	theme	alginate	119:126	arg1	films					128:132	alginate films	119:132	alginate films	119:132	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	11	37	theme	wound	1518:1522	arg1	dressing					1524:1531	wound dressing	1518:1531	wound dressing	1518:1531	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
31945444	4	38	theme	crosslinked	685:695	arg1	films					722:726	crosslinked and plasticized alginate films	685:726	crosslinked and plasticized alginate films	685:726	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	3	39	theme	concentrations	526:539	arg1	variation					476:484	variation	476:484	variation of both the plasticizer and crosslinker concentrations in twenty different compositions	476:572	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	2	40	theme	solutions	400:408	arg1	concentrations					365:378	four different concentrations	350:378	four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w)	350:431	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	6	41	theme	good	1092:1095	arg1	strength					1097:1104	good strength	1092:1104	good strength	1092:1104	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	3	42	theme	first	446:450	arg1	This					434:437	This	434:437	This	434:437	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	3	42	theme	first	446:450	arg1	investigation					452:464	the first investigation	442:464	the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions	442:572	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	8	43	link	crosslinked	1202:1212	arg1	films					1228:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	5	44	theme	tensile	876:882	arg1	behavior					884:891	tensile behavior	876:891	tensile behavior	876:891	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	1	45	theme	biodegradable	201:213	arg1	nature					215:220	their biocompatible and biodegradable nature	177:220	their biocompatible and biodegradable nature	177:220	Alginates attract growing interest due to their biocompatible and biodegradable nature.					
31945444	0	46	theme	effect	32:37	arg1	investigation					11:23	A detailed investigation	0:23	A detailed investigation of the effect of calcium crosslinking and glycerol	0:74	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	6	47	theme	combined	1038:1045	arg1	effect					1047:1052	a combined effect	1036:1052	a combined effect	1036:1052	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	11	48	contain	have	1485:1488	arg2	potential					1505:1513	an appreciable potential	1490:1513	an appreciable potential	1490:1513	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
31945444	11	48	contain	have	1485:1488	arg1	films					1479:1483	alginate films	1470:1483	alginate films	1470:1483	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
31945444	2	49	theme	wide	231:234	arg1	spectrum					236:243	a wide spectrum	229:243	a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate)	229:315	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	8	50	theme	Low	1198:1200	arg1	films					1228:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	2	51	theme	calcium	383:389	arg1	solutions					400:408	calcium chloride solutions	383:408	calcium chloride solutions (0.5, 1, 1.5, 2%, w/w)	383:431	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	4	52	theme	synergic	620:627	arg1	effect					629:634	a synergic effect	618:634	a synergic effect on the mechanical properties	618:663	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	9	53	theme	films	1319:1323	arg1	permeability					1303:1314	Water vapor permeability	1291:1314	Water vapor permeability of films	1291:1323	Water vapor permeability of films decreased regularly with increasing calcium concentration.					
31945444	0	54	theme	films	128:132	arg1	properties					105:114	the physical properties	92:114	the physical properties of alginate films	92:132	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	0	55	theme	calcium	42:48	arg1	crosslinking					50:61	calcium crosslinking	42:61	calcium crosslinking	42:61	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	6	56	with	combinations	1074:1085	arg1	strength					1097:1104	good strength	1092:1104	good strength	1092:1104	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	6	56	with	combinations	1074:1085	arg1	properties					1126:1135	fracture strain properties	1110:1135	fracture strain properties	1110:1135	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	5	57	from	properties	978:987	arg1	evident					956:962	evident	956:962	evident	956:962	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	2	58	dep	solutions	400:408	arg1	w/w					428:430	w/w	428:430	w/w	428:430	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	58	dep	solutions	400:408	arg1	%					425:425	0.5, 1, 1.5, 2%	411:425	0.5, 1, 1.5, 2%	411:425	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	5	59	contain	had	849:851	arg1	crosslinking					836:847	crosslinking	836:847	crosslinking	836:847	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	5	59	contain	had	849:851	arg2	effect					866:871	a negligible effect	853:871	a negligible effect	853:871	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	4	60	theme	mechanical	643:652	arg1	properties					654:663	the mechanical properties	639:663	the mechanical properties	639:663	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	3	61	theme	crosslinker	514:524	arg1	concentrations					526:539	both the plasticizer and crosslinker concentrations	489:539	concentrations	526:539	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	11	62	theme	alginate	1470:1477	arg1	films					1479:1483	alginate films	1470:1483	alginate films	1470:1483	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
31945444	11	63	theme	packaging	1542:1550	arg1	applications					1552:1563	food packaging applications	1537:1563	food packaging applications	1537:1563	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
31945444	4	64	contain	have	613:616	arg1	calcium					605:611	calcium	605:611	calcium	605:611	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	4	64	contain	have	613:616	arg1	glycerol					592:599	glycerol	592:599	glycerol	592:599	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	4	64	contain	have	613:616	arg2	effect					629:634	a synergic effect	618:634	a synergic effect on the mechanical properties	618:663	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	2	65	theme	added	257:261	arg1	glycerol/alginate					298:314	glycerol/alginate	298:314	glycerol/alginate	298:314	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	65	theme	added	257:261	arg1	films					272:276	glycerol added alginate films	248:276	glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate)	248:315	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	8	66	theme	swelling	1261:1268	arg1	films					1270:1274	highly crosslinked low swelling films	1238:1274	highly crosslinked low swelling films	1238:1274	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	4	67	theme	films	722:726	arg1	behavior					673:680	the behavior	669:680	the behavior of crosslinked and plasticized alginate films	669:726	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	5	68	theme	mechanical	967:976	arg1	properties					978:987	mechanical properties	967:987	mechanical properties	967:987	Without glycerol, crosslinking had a negligible effect on tensile behavior, but with glycerol addition, the effect of crosslinking became evident in mechanical properties.					
31945444	2	69	theme	glycerol	248:255	arg1	glycerol/alginate					298:314	glycerol/alginate	298:314	glycerol/alginate	298:314	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	69	theme	glycerol	248:255	arg1	films					272:276	glycerol added alginate films	248:276	glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate)	248:315	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	2	70	dep	30	289:290	arg1	to					286:287	to	286:287	to	286:287	Here, a wide spectrum of glycerol added alginate films (from 0 to 30% w/w, glycerol/alginate) were prepared and crosslinked by four different concentrations of calcium chloride solutions (0.5, 1, 1.5, 2%, w/w).					
31945444	9	71	theme	calcium	1361:1367	arg1	concentration					1369:1381	calcium concentration	1361:1381	calcium concentration	1361:1381	Water vapor permeability of films decreased regularly with increasing calcium concentration.					
31945444	4	72	from	effect	629:634	arg1	properties					654:663	the mechanical properties	639:663	the mechanical properties	639:663	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	8	73	theme	swelling	1219:1226	arg1	films					1228:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films	1198:1232	Low crosslinked high swelling films and highly crosslinked low swelling films were prepared.					
31945444	4	74	link	crosslinked	685:695	arg1	films					722:726	crosslinked and plasticized alginate films	685:726	crosslinked and plasticized alginate films	685:726	It is shown that glycerol and calcium have a synergic effect on the mechanical properties and the behavior of crosslinked and plasticized alginate films cannot be predicted by studies, which vary only one of these, keeping the other constant.					
31945444	6	75	theme	fracture	1110:1117	arg1	properties					1126:1135	fracture strain properties	1110:1135	fracture strain properties	1110:1135	Calcium and glycerol concentrations exhibited a combined effect, displaying optimum combinations with good strength and fracture strain properties.					
31945444	0	76	theme	crosslinking	50:61	arg1	effect					32:37	the effect	28:37	the effect of calcium crosslinking and glycerol	28:74	A detailed investigation of the effect of calcium crosslinking and glycerol plasticizing on the physical properties of alginate films.					
31945444	3	77	theme	different	551:559	arg1	compositions					561:572	twenty different compositions	544:572	twenty different compositions	544:572	This is the first investigation involving variation of both the plasticizer and crosslinker concentrations in twenty different compositions.					
31945444	11	78	theme	appreciable	1493:1503	arg1	potential					1505:1513	an appreciable potential	1490:1513	an appreciable potential	1490:1513	The results showed that alginate films have an appreciable potential in wound dressing and food packaging applications.					
33292964	4	0	contain	had	614:616	arg2	11.7 g/100 g					618:629	11.7 g/100 g	618:629	11.7 g/100 g of dietary fibre	618:646	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	4	0	contain	had	614:616	arg1	flour					608:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	0	1	theme	fibre	141:145	arg1	source					99:104	an alternative source	84:104	an alternative source of starch, polyphenols and dietary fibre	84:145	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	8	2	theme	fibre	1536:1540	arg1	content					1542:1548	high dietary fibre content	1523:1548	high dietary fibre content	1523:1548	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	1	3	theme	mangoes	215:221	arg1	production					183:192	the production	179:192	the production of stenospermocarpic mangoes	179:221	As a result of climate change, the production of stenospermocarpic mangoes has increased dramatically.					
33292964	7	4	theme	pulp	1335:1338	arg1	flour					1340:1344	the mango pulp flour	1325:1344	the mango pulp flour (approximately 20% per fraction)	1325:1377	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	5	theme	mango	597:601	arg1	flour					608:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	8	6	used	used	1455:1458	arg2	ingredients					1475:1485	alternative ingredients	1463:1485	alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities	1463:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	8	6	used	used	1455:1458	arg2	flours					1384:1389	The flours	1380:1389	The flours of the pulp and peel of unripe stenospermocarpic mangoes	1380:1446	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	3	7	theme	antioxidant	462:472	arg1	capacity					474:481	antioxidant capacity	462:481	antioxidant capacity	462:481	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	7	8	theme	fractions	1312:1320	arg1	content					1273:1279	the content	1269:1279	the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction)	1269:1377	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	9	theme	resistant	775:783	arg1	flour					768:772	its uncooked flour	755:772	its uncooked flour (resistant starch)	755:791	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	4	9	theme	resistant	775:783	arg1	starch					785:790	resistant starch	775:790	resistant starch	775:790	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	5	10	contain	had	879:881	arg2	content					942:948	higher dietary fibre (40.5 g/100 g) and lower total starch content	883:948	higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g)	883:961	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	10	contain	had	879:881	arg1	flour					873:877	The mango peel flour	858:877	The mango peel flour	858:877	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	10	contain	had	879:881	arg2	21 g/100 g					951:960	21 g/100 g	951:960	21 g/100 g	951:960	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	6	11	theme	peel	1129:1132	arg1	flour					1134:1138	the peel flour	1125:1138	the peel flour (16.51 mg/g and 92.08 mg/g, DPPH)	1125:1172	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	8	12	theme	antioxidant	1580:1590	arg1	capacities					1592:1601	antioxidant capacities	1580:1601	antioxidant capacities	1580:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	4	13	theme	indigestible	830:841	arg1	carbohydrates					843:855	the indigestible carbohydrates	826:855	the indigestible carbohydrates	826:855	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	0	14	theme	alternative	87:97	arg1	source					99:104	an alternative source	84:104	an alternative source of starch, polyphenols and dietary fibre	84:145	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	3	15	theme	in	494:495	arg1	digestibility					503:515	starch in vitro digestibility	487:515	starch in vitro digestibility	487:515	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	6	16	contain	had	1016:1018	arg2	capacity					1083:1090	antioxidant capacity	1071:1090	antioxidant capacity (248.5 mg/g, DPPH)	1071:1109	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	16	contain	had	1016:1018	arg2	99.71 mg/g					1055:1064	99.71 mg/g	1055:1064	99.71 mg/g	1055:1064	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	16	contain	had	1016:1018	arg2	content					1046:1052	higher phenolic compounds content	1020:1052	higher phenolic compounds content (99.71 mg/g)	1020:1065	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	16	contain	had	1016:1018	arg1	flour					1010:1014	The mango pulp flour	995:1014	The mango pulp flour	995:1014	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	17	dep	capacity	1083:1090	arg1	DPPH					1105:1108	DPPH	1105:1108	DPPH	1105:1108	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	17	dep	capacity	1083:1090	arg1	248.5 mg/g					1093:1102	248.5 mg/g	1093:1102	248.5 mg/g	1093:1102	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	5	18	theme	pulp	983:986	arg1	flour					988:992	mango pulp flour	977:992	mango pulp flour	977:992	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	3	19	theme	mango	545:549	arg1	peel					568:571	peel	568:571	peel	568:571	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	19	theme	mango	545:549	arg1	pulp					559:562	pulp	559:562	pulp	559:562	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	19	theme	mango	545:549	arg1	flours					551:556	unripe stenospermocarpic mango flours	520:556	unripe stenospermocarpic mango flours (pulp and peel)	520:572	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	1	20	theme	change	171:176	arg1	result					153:158	a result	151:158	a result of climate change	151:176	As a result of climate change, the production of stenospermocarpic mangoes has increased dramatically.					
33292964	8	21	dep	pulp	1398:1401	arg1	the					1394:1396	the	1394:1396	the	1394:1396	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	3	22	theme	unripe	520:525	arg1	peel					568:571	peel	568:571	peel	568:571	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	22	theme	unripe	520:525	arg1	pulp					559:562	pulp	559:562	pulp	559:562	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	22	theme	unripe	520:525	arg1	flours					551:556	unripe stenospermocarpic mango flours	520:556	unripe stenospermocarpic mango flours (pulp and peel)	520:572	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	7	23	from	balance	1258:1264	arg1	content					1273:1279	the content	1269:1279	the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction)	1269:1377	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	24	from	content	730:736	arg1	flour					768:772	its uncooked flour	755:772	its uncooked flour (resistant starch)	755:791	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	4	24	from	content	730:736	arg1	starch					785:790	resistant starch	775:790	resistant starch	775:790	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	3	25	dep	in	494:495	arg1	vitro					497:501	vitro	497:501	vitro	497:501	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	8	26	with	compounds	1565:1573	arg1	capacities					1592:1601	antioxidant capacities	1580:1601	antioxidant capacities	1580:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	6	27	theme	antioxidant	1071:1081	arg1	capacity					1083:1090	antioxidant capacity	1071:1090	antioxidant capacity (248.5 mg/g, DPPH)	1071:1109	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	3	28	dep	colour	412:417	arg1	the					408:410	the	408:410	the	408:410	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	7	29	theme	starch	1212:1217	arg1	fraction					1219:1226	The rapidly digestible starch fraction	1189:1226	The rapidly digestible starch fraction	1189:1226	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	30	theme	soluble	666:672	arg1	fractions					688:696	soluble and insoluble fractions	666:696	soluble and insoluble fractions	666:696	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	5	31	theme	mango	862:866	arg1	flour					873:877	The mango peel flour	858:877	The mango peel flour	858:877	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	4	32	theme	insoluble	678:686	arg1	fractions					688:696	soluble and insoluble fractions	666:696	soluble and insoluble fractions	666:696	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	7	33	dep	flour	1340:1344	arg1	%					1363:1363	approximately 20%	1347:1363	approximately 20% per fraction	1347:1376	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	34	theme	41 g/100 g	741:750	arg1	content					730:736	the total starch content	713:736	the total starch content of 41 g/100 g in its uncooked flour (resistant starch)	713:791	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	0	35	theme	unripe	17:22	arg1	mango					42:46	unripe stenospermocarpic mango	17:46	unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo)	17:79	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	0	35	theme	unripe	17:22	arg1	Ataulfo					72:78	Ataulfo	72:78	Ataulfo	72:78	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	5	36	theme	total	929:933	arg1	21 g/100 g					951:960	21 g/100 g	951:960	21 g/100 g	951:960	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	36	theme	total	929:933	arg1	content					942:948	higher dietary fibre (40.5 g/100 g) and lower total starch content	883:948	higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g)	883:961	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	0	37	theme	mango	42:46	arg1	peel					9:12	peel	9:12	peel	9:12	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	0	37	theme	mango	42:46	arg1	Pulp					0:3	Pulp	0:3	Pulp	0:3	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	6	38	theme	higher	1020:1025	arg1	99.71 mg/g					1055:1064	99.71 mg/g	1055:1064	99.71 mg/g	1055:1064	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	38	theme	higher	1020:1025	arg1	content					1046:1052	higher phenolic compounds content	1020:1052	higher phenolic compounds content (99.71 mg/g)	1020:1065	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	4	39	theme	total	717:721	arg1	content					730:736	the total starch content	713:736	the total starch content of 41 g/100 g in its uncooked flour (resistant starch)	713:791	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	2	40	theme	reduced	293:299	arg1	size					301:304	reduced size	293:304	reduced size	293:304	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	8	41	theme	stenospermocarpic	1422:1438	arg1	mangoes					1440:1446	unripe stenospermocarpic mangoes	1415:1446	unripe stenospermocarpic mangoes	1415:1446	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	3	42	theme	polyphenol	442:451	arg1	content					453:459	polyphenol content	442:459	polyphenol content	442:459	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	8	43	theme	functional	1501:1510	arg1	foods					1512:1516	functional foods	1501:1516	functional foods	1501:1516	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	6	44	theme	mango	999:1003	arg1	flour					1010:1014	The mango pulp flour	995:1014	The mango pulp flour	995:1014	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	1	45	theme	stenospermocarpic	197:213	arg1	mangoes					215:221	stenospermocarpic mangoes	197:221	stenospermocarpic mangoes	197:221	As a result of climate change, the production of stenospermocarpic mangoes has increased dramatically.					
33292964	7	46	theme	mango	1329:1333	arg1	flour					1340:1344	the mango pulp flour	1325:1344	the mango pulp flour (approximately 20% per fraction)	1325:1377	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	2	47	theme	stenospermocarpic	255:271	arg1	underutilised					365:377	underutilised	365:377	underutilised	365:377	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	2	47	theme	stenospermocarpic	255:271	arg1	by-product					341:350	a by-product	339:350	a by-product that is both underutilised and wasted	339:388	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	2	47	theme	stenospermocarpic	255:271	arg1	mango					273:277	The stenospermocarpic mango	251:277	The stenospermocarpic mango	251:277	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	2	47	theme	stenospermocarpic	255:271	arg1	fruit					282:286	a fruit	280:286	a fruit with reduced size and no seed	280:316	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	8	48	theme	dietary	1528:1534	arg1	content					1542:1548	high dietary fibre content	1523:1548	high dietary fibre content	1523:1548	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	7	49	theme	resistant	1295:1303	arg1	fractions					1312:1320	slowly and resistant starch fractions	1284:1320	slowly and resistant starch fractions	1284:1320	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	8	50	theme	alternative	1463:1473	arg1	flours					1384:1389	The flours	1380:1389	The flours of the pulp and peel of unripe stenospermocarpic mangoes	1380:1446	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	8	50	theme	alternative	1463:1473	arg1	ingredients					1475:1485	alternative ingredients	1463:1485	alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities	1463:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	7	51	theme	starch	1305:1310	arg1	fractions					1312:1320	slowly and resistant starch fractions	1284:1320	slowly and resistant starch fractions	1284:1320	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	52	theme	uncooked	759:766	arg1	flour					768:772	its uncooked flour	755:772	its uncooked flour (resistant starch)	755:791	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	4	52	theme	uncooked	759:766	arg1	starch					785:790	resistant starch	775:790	resistant starch	775:790	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	7	53	theme	slowly	1284:1289	arg1	fractions					1312:1320	slowly and resistant starch fractions	1284:1320	slowly and resistant starch fractions	1284:1320	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	4	54	theme	stenospermocarpic	579:595	arg1	flour					608:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	8	55	theme	polyphenol	1554:1563	arg1	compounds					1565:1573	polyphenol compounds	1554:1573	polyphenol compounds with antioxidant capacities	1554:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	4	56	theme	pulp	603:606	arg1	flour					608:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour	575:612	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	3	57	theme	starch	487:492	arg1	digestibility					503:515	starch in vitro digestibility	487:515	starch in vitro digestibility	487:515	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	58	dep	flours	551:556	arg1	peel					568:571	peel	568:571	peel	568:571	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	58	dep	flours	551:556	arg1	pulp					559:562	pulp	559:562	pulp	559:562	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	58	dep	flours	551:556	arg1	flours					551:556	unripe stenospermocarpic mango flours	520:556	unripe stenospermocarpic mango flours (pulp and peel)	520:572	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	6	59	theme	compounds	1036:1044	arg1	99.71 mg/g					1055:1064	99.71 mg/g	1055:1064	99.71 mg/g	1055:1064	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	59	theme	compounds	1036:1044	arg1	content					1046:1052	higher phenolic compounds content	1020:1052	higher phenolic compounds content (99.71 mg/g)	1020:1065	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	0	60	theme	starch	109:114	arg1	source					99:104	an alternative source	84:104	an alternative source of starch, polyphenols and dietary fibre	84:145	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	5	61	theme	mango	977:981	arg1	flour					988:992	mango pulp flour	977:992	mango pulp flour	977:992	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	3	62	theme	stenospermocarpic	527:543	arg1	peel					568:571	peel	568:571	peel	568:571	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	62	theme	stenospermocarpic	527:543	arg1	pulp					559:562	pulp	559:562	pulp	559:562	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	62	theme	stenospermocarpic	527:543	arg1	flours					551:556	unripe stenospermocarpic mango flours	520:556	unripe stenospermocarpic mango flours (pulp and peel)	520:572	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	1	63	theme	climate	163:169	arg1	change					171:176	climate change	163:176	climate change	163:176	As a result of climate change, the production of stenospermocarpic mangoes has increased dramatically.					
33292964	0	64	theme	polyphenols	117:127	arg1	source					99:104	an alternative source	84:104	an alternative source of starch, polyphenols and dietary fibre	84:145	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	4	65	theme	fibre	642:646	arg1	11.7 g/100 g					618:629	11.7 g/100 g	618:629	11.7 g/100 g of dietary fibre	618:646	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	4	66	from	increase	814:821	arg1	carbohydrates					843:855	the indigestible carbohydrates	826:855	the indigestible carbohydrates	826:855	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	5	67	dep	higher	883:888	arg1	40.5 g/100 g					905:916	40.5 g/100 g	905:916	40.5 g/100 g	905:916	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	67	dep	higher	883:888	arg1	fibre					898:902	fibre	898:902	fibre	898:902	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	3	68	theme	flours	551:556	arg1	capacity					474:481	antioxidant capacity	462:481	antioxidant capacity	462:481	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	68	theme	flours	551:556	arg1	digestibility					503:515	starch in vitro digestibility	487:515	starch in vitro digestibility	487:515	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	68	theme	flours	551:556	arg1	colour					412:417	colour	412:417	colour	412:417	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	68	theme	flours	551:556	arg1	composition					429:439	chemical composition	420:439	chemical composition	420:439	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	3	68	theme	flours	551:556	arg1	content					453:459	polyphenol content	442:459	polyphenol content	442:459	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	7	69	from	content	1273:1279	arg1	flour					1340:1344	the mango pulp flour	1325:1344	the mango pulp flour (approximately 20% per fraction)	1325:1377	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	8	70	with	content	1542:1548	arg1	capacities					1592:1601	antioxidant capacities	1580:1601	antioxidant capacities	1580:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	7	71	with	%	1248:1248	arg1	balance					1258:1264	a balance	1256:1264	a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction)	1256:1377	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	2	72	with	fruit	282:286	arg1	size					301:304	reduced size	293:304	reduced size	293:304	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	2	72	with	fruit	282:286	arg1	seed					313:316	seed	313:316	seed	313:316	The stenospermocarpic mango, a fruit with reduced size and no seed, is considered to be a by-product that is both underutilised and wasted.					
33292964	5	73	theme	higher	883:888	arg1	21 g/100 g					951:960	21 g/100 g	951:960	21 g/100 g	951:960	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	73	theme	higher	883:888	arg1	content					942:948	higher dietary fibre (40.5 g/100 g) and lower total starch content	883:948	higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g)	883:961	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	4	74	theme	fractions	688:696	arg1	balance					655:661	a balance	653:661	a balance of soluble and insoluble fractions	653:696	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	7	75	theme	digestible	1201:1210	arg1	starch					1212:1217	The rapidly digestible starch	1189:1217	The rapidly digestible starch fraction	1189:1226	The rapidly digestible starch fraction was approximately 50%, with a balance in the content of slowly and resistant starch fractions in the mango pulp flour (approximately 20% per fraction).					
33292964	5	76	theme	peel	868:871	arg1	flour					873:877	The mango peel flour	858:877	The mango peel flour	858:877	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	8	77	theme	pulp	1398:1401	arg1	flours					1384:1389	The flours	1380:1389	The flours of the pulp and peel of unripe stenospermocarpic mangoes	1380:1446	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	8	77	theme	pulp	1398:1401	arg1	ingredients					1475:1485	alternative ingredients	1463:1485	alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities	1463:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	0	78	theme	stenospermocarpic	24:40	arg1	mango					42:46	unripe stenospermocarpic mango	17:46	unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo)	17:79	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	0	78	theme	stenospermocarpic	24:40	arg1	Ataulfo					72:78	Ataulfo	72:78	Ataulfo	72:78	Pulp and peel of unripe stenospermocarpic mango (Mangifera indica L. cv Ataulfo) as an alternative source of starch, polyphenols and dietary fibre.					
33292964	5	79	theme	lower	923:927	arg1	21 g/100 g					951:960	21 g/100 g	951:960	21 g/100 g	951:960	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	79	theme	lower	923:927	arg1	content					942:948	higher dietary fibre (40.5 g/100 g) and lower total starch content	883:948	higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g)	883:961	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	8	80	theme	peel	1407:1410	arg1	flours					1384:1389	The flours	1380:1389	The flours of the pulp and peel of unripe stenospermocarpic mangoes	1380:1446	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	8	80	theme	peel	1407:1410	arg1	ingredients					1475:1485	alternative ingredients	1463:1485	alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities	1463:1601	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	5	81	theme	starch	935:940	arg1	21 g/100 g					951:960	21 g/100 g	951:960	21 g/100 g	951:960	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	5	81	theme	starch	935:940	arg1	content					942:948	higher dietary fibre (40.5 g/100 g) and lower total starch content	883:948	higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g)	883:961	The mango peel flour had higher dietary fibre (40.5 g/100 g) and lower total starch content (21 g/100 g) compared with mango pulp flour.					
33292964	6	82	theme	phenolic	1027:1034	arg1	99.71 mg/g					1055:1064	99.71 mg/g	1055:1064	99.71 mg/g	1055:1064	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	82	theme	phenolic	1027:1034	arg1	content					1046:1052	higher phenolic compounds content	1020:1052	higher phenolic compounds content (99.71 mg/g)	1020:1065	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	8	83	theme	unripe	1415:1420	arg1	mangoes					1440:1446	unripe stenospermocarpic mangoes	1415:1446	unripe stenospermocarpic mangoes	1415:1446	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	3	84	theme	chemical	420:427	arg1	composition					429:439	chemical composition	420:439	chemical composition	420:439	Here, we studied the colour, chemical composition, polyphenol content, antioxidant capacity and starch in vitro digestibility of unripe stenospermocarpic mango flours (pulp and peel).					
33292964	8	85	theme	mangoes	1440:1446	arg1	peel					1407:1410	peel	1407:1410	peel	1407:1410	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	8	85	theme	mangoes	1440:1446	arg1	pulp					1398:1401	pulp	1398:1401	pulp	1398:1401	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
33292964	6	86	dep	flour	1134:1138	arg1	92.08 mg/g					1156:1165	92.08 mg/g	1156:1165	92.08 mg/g	1156:1165	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	86	dep	flour	1134:1138	arg1	16.51 mg/g					1141:1150	16.51 mg/g	1141:1150	16.51 mg/g	1141:1150	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	86	dep	flour	1134:1138	arg1	DPPH					1168:1171	DPPH	1168:1171	DPPH	1168:1171	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	6	87	theme	pulp	1005:1008	arg1	flour					1010:1014	The mango pulp flour	995:1014	The mango pulp flour	995:1014	The mango pulp flour had higher phenolic compounds content (99.71 mg/g) and antioxidant capacity (248.5 mg/g, DPPH) compared with the peel flour (16.51 mg/g and 92.08 mg/g, DPPH), respectively.					
33292964	4	88	theme	starch	723:728	arg1	content					730:736	the total starch content	713:736	the total starch content of 41 g/100 g in its uncooked flour (resistant starch)	713:791	The stenospermocarpic mango pulp flour had 11.7 g/100 g of dietary fibre with a balance of soluble and insoluble fractions; additionally, the total starch content of 41 g/100 g in its uncooked flour (resistant starch) can contribute to an increase in the indigestible carbohydrates.					
33292964	8	89	theme	high	1523:1526	arg1	content					1542:1548	high dietary fibre content	1523:1548	high dietary fibre content	1523:1548	The flours of the pulp and peel of unripe stenospermocarpic mangoes can be used as alternative ingredients for preparing functional foods with high dietary fibre content and polyphenol compounds with antioxidant capacities.					
34116153	10	0	dep	available	1917:1925	arg1	CBV-MRI					1897:1903	CBV-MRI	1897:1903	CBV-MRI	1897:1903	It is our hope that this work makes CBV-MRI more openly available and affordable for a variety of research applications.					
34116153	10	1	theme	applications	1968:1979	arg1	variety					1948:1954	a variety	1946:1954	a variety of research applications	1946:1979	It is our hope that this work makes CBV-MRI more openly available and affordable for a variety of research applications.					
34116153	10	1	theme	applications	1968:1979	arg1	applications					1968:1979	research applications	1959:1979	research applications	1959:1979	It is our hope that this work makes CBV-MRI more openly available and affordable for a variety of research applications.					
34116153	1	2	theme	functional	266:275	arg1	mapping					277:283	sensitive structural and functional mapping	241:283	sensitive structural and functional mapping of the cerebral blood volume (CBV)	241:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	9	3	theme	synthesis	1720:1728	arg1	method					1730:1735	our one-pot synthesis method	1708:1735	our one-pot synthesis method	1708:1735	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	6	4	theme	MR	1197:1198	arg1	relaxivity					1200:1209	MR relaxivity	1197:1209	MR relaxivity	1197:1209	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	3	5	theme	commercial	556:565	arg1	constraints					582:592	commercial and regulatory constraints	556:592	commercial and regulatory constraints	556:592	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	7	6	theme	structural	1345:1354	arg1	stimulation					1452:1462	targeted optogenetic stimulation	1431:1462	targeted optogenetic stimulation	1431:1462	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	6	theme	structural	1345:1354	arg1	microangiography					1469:1484	microangiography	1469:1484	microangiography	1469:1484	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	6	theme	structural	1345:1354	arg1	challenge					1400:1408	hypercapnic CO2 challenge	1384:1408	hypercapnic CO2 challenge	1384:1408	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	6	theme	structural	1345:1354	arg1	applications					1360:1371	functional and structural MRI applications	1330:1371	functional and structural MRI applications	1330:1371	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	6	theme	structural	1345:1354	arg1	stimulation					1418:1428	visual stimulation	1411:1428	visual stimulation	1411:1428	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	3	7	theme	due	549:551	arg1	States					542:547	the United States	531:547	the United States due to commercial and regulatory constraints	531:592	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	1	8	theme	resonance	208:216	arg1	MRI					227:229	MRI	227:229	MRI	227:229	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	8	theme	resonance	208:216	arg1	imaging					218:224	magnetic resonance imaging	199:224	magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV)	199:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	7	9	theme	targeted	1431:1438	arg1	stimulation					1452:1462	targeted optogenetic stimulation	1431:1462	targeted optogenetic stimulation	1431:1462	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	9	10	dep	imaging	1846:1852	arg1	needs					1854:1858	needs	1854:1858	needs	1854:1858	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	3	11	theme	regulatory	571:580	arg1	constraints					582:592	commercial and regulatory constraints	556:592	commercial and regulatory constraints	556:592	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	6	12	theme	metal	1087:1091	arg1	ratio					1108:1112	metal stoichiometric ratio	1087:1112	metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition	1087:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	9	13	dep	simplicity	1671:1680	arg1	The					1667:1669	The	1667:1669	The	1667:1669	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	3	14	theme	CBV-MRI	602:608	arg1	methods					610:616	CBV-MRI methods	602:616	CBV-MRI methods either inaccessible or very costly	602:651	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	5	15	theme	protocol	932:939	arg1	instruction					895:905	a step-by-step instruction	880:905	a step-by-step instruction	880:905	Here we disseminate a step-by-step instruction of our one-pot synthesis protocol, which allows CION to be produced in laboratories with minimal cost.					
34116153	7	16	theme	CO2	1396:1398	arg1	challenge					1400:1408	hypercapnic CO2 challenge	1384:1408	hypercapnic CO2 challenge	1384:1408	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	3	17	theme	costly	646:651	arg1	methods					610:616	CBV-MRI methods	602:616	CBV-MRI methods either inaccessible or very costly	602:651	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	1	18	theme	iron-oxide	143:152	arg1	agents					188:193	robust contrast agents	172:193	robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV)	172:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	18	theme	iron-oxide	143:152	arg1	nanoparticles					154:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	6	19	theme	different	1032:1040	arg1	CION-conjugations					1042:1058	different CION-conjugations	1032:1058	different CION-conjugations	1032:1058	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	7	20	theme	proof-of-concept	1254:1269	arg1	experiments					1271:1281	several proof-of-concept experiments	1246:1281	several proof-of-concept experiments	1246:1281	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	4	21	theme	simple	705:710	arg1	recipe					721:726	a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles	703:794	a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles	703:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	1	22	theme	contrast	179:186	arg1	agents					188:193	robust contrast agents	172:193	robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV)	172:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	22	theme	contrast	179:186	arg1	nanoparticles					154:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	8	23	theme	research	1552:1559	arg1	platform					1561:1568	a cross-modality research platform	1535:1568	a cross-modality research platform	1535:1568	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	8	23	theme	research	1552:1559	arg1	CION					1517:1520	CION	1517:1520	CION	1517:1520	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	0	24	theme	preclinical	86:96	arg1	fMRI					98:101	preclinical fMRI and MRA applications	86:122	fMRI	98:101	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	9	25	dep	relaxivity	1795:1804	arg1	the					1791:1793	the	1791:1793	the	1791:1793	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	8	26	theme	concurrent	1581:1590	arg1	optical					1600:1606	concurrent in vivo optical	1581:1606	concurrent in vivo optical	1581:1606	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	4	27	theme	Carboxymethyl-dextran	742:762	arg1	Nanoparticles					782:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	0	28	theme	One-pot	0:6	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of carboxymethyl-dextran	0:41	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	7	29	theme	MRI	1356:1358	arg1	stimulation					1452:1462	targeted optogenetic stimulation	1431:1462	targeted optogenetic stimulation	1431:1462	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	29	theme	MRI	1356:1358	arg1	microangiography					1469:1484	microangiography	1469:1484	microangiography	1469:1484	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	29	theme	MRI	1356:1358	arg1	challenge					1400:1408	hypercapnic CO2 challenge	1384:1408	hypercapnic CO2 challenge	1384:1408	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	29	theme	MRI	1356:1358	arg1	applications					1360:1371	functional and structural MRI applications	1330:1371	functional and structural MRI applications	1330:1371	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	29	theme	MRI	1356:1358	arg1	stimulation					1418:1428	visual stimulation	1411:1428	visual stimulation	1411:1428	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	2	30	theme	clinical	433:440	arg1	treatment					442:450	the clinical treatment	429:450	the clinical treatment of iron-deficiency	429:469	To date, many CBV-MRI studies are conducted with Feraheme, manufactured for the clinical treatment of iron-deficiency.					
34116153	4	31	theme	Iron	771:774	arg1	Nanoparticles					782:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	1	32	theme	blood	301:305	arg1	CBV					315:317	CBV	315:317	CBV	315:317	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	32	theme	blood	301:305	arg1	volume					307:312	the cerebral blood volume	288:312	the cerebral blood volume (CBV)	288:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	4	33	theme	suitable	813:820	arg1	recipe					721:726	a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles	703:794	a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles	703:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	4	34	theme	preclinical	826:836	arg1	applications					846:857	preclinical CBV-MRI applications	826:857	preclinical CBV-MRI applications	826:857	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	0	35	theme	oxide	55:59	arg1	CION					76:79	CION	76:79	CION	76:79	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	0	35	theme	oxide	55:59	arg1	nanoparticles					61:73	iron oxide nanoparticles	50:73	iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications	50:122	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	6	36	theme	chemistry	1146:1154	arg1	terms					1117:1121	terms	1117:1121	terms of their size, surface chemistry, and chemical composition	1117:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	8	37	theme	CBV	1631:1633	arg1	optical					1600:1606	concurrent in vivo optical	1581:1606	concurrent in vivo optical	1581:1606	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	8	37	theme	CBV	1631:1633	arg1	measurement					1616:1626	MRI measurement	1612:1626	MRI measurement	1612:1626	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	8	38	dep	in	1592:1593	arg1	vivo					1595:1598	vivo	1595:1598	vivo	1595:1598	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	8	39	theme	fluorescent-labeled	1641:1659	arg1	CION					1661:1664	fluorescent-labeled CION	1641:1664	fluorescent-labeled CION	1641:1664	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	4	40	theme	"	810:810	arg1	recipe					721:726	a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles	703:794	a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles	703:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	2	41	theme	CBV-MRI	367:373	arg1	studies					375:381	many CBV-MRI studies	362:381	many CBV-MRI studies	362:381	To date, many CBV-MRI studies are conducted with Feraheme, manufactured for the clinical treatment of iron-deficiency.					
34116153	1	42	theme	sensitive	241:249	arg1	mapping					277:283	sensitive structural and functional mapping	241:283	sensitive structural and functional mapping of the cerebral blood volume (CBV)	241:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	9	43	theme	one-pot	1712:1718	arg1	method					1730:1735	our one-pot synthesis method	1708:1735	our one-pot synthesis method	1708:1735	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	10	44	theme	research	1959:1966	arg1	applications					1968:1979	research applications	1959:1979	research applications	1959:1979	It is our hope that this work makes CBV-MRI more openly available and affordable for a variety of research applications.					
34116153	9	45	theme	method	1730:1735	arg1	cost-effectiveness					1686:1703	cost-effectiveness	1686:1703	cost-effectiveness	1686:1703	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	9	45	theme	method	1730:1735	arg1	simplicity					1671:1680	simplicity	1671:1680	simplicity	1671:1680	The simplicity and cost-effectiveness of our one-pot synthesis method should allow researchers to reproduce CION and tailor the relaxivity and pharmacokinetics according to their imaging needs.					
34116153	6	46	from	ratio	1108:1112	arg1	terms					1117:1121	terms	1117:1121	terms of their size, surface chemistry, and chemical composition	1117:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	4	47	theme	CBV-MRI	838:844	arg1	applications					846:857	preclinical CBV-MRI applications	826:857	preclinical CBV-MRI applications	826:857	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	1	48	theme	magnetic	199:206	arg1	MRI					227:229	MRI	227:229	MRI	227:229	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	48	theme	magnetic	199:206	arg1	imaging					218:224	magnetic resonance imaging	199:224	magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV)	199:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	6	49	theme	composition	1170:1180	arg1	terms					1117:1121	terms	1117:1121	terms of their size, surface chemistry, and chemical composition	1117:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	6	50	from	polymer	1076:1082	arg1	pharmacokinetics					1215:1230	pharmacokinetics	1215:1230	pharmacokinetics	1215:1230	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	6	50	from	polymer	1076:1082	arg1	relaxivity					1200:1209	MR relaxivity	1197:1209	MR relaxivity	1197:1209	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	5	51	theme	one-pot	914:920	arg1	protocol					932:939	our one-pot synthesis protocol	910:939	our one-pot synthesis protocol	910:939	Here we disseminate a step-by-step instruction of our one-pot synthesis protocol, which allows CION to be produced in laboratories with minimal cost.					
34116153	10	52	dep	our	1867:1869	arg1	hope					1871:1874	hope	1871:1874	hope	1871:1874	It is our hope that this work makes CBV-MRI more openly available and affordable for a variety of research applications.					
34116153	5	53	with	laboratories	978:989	arg1	cost					1004:1007	minimal cost	996:1007	minimal cost	996:1007	Here we disseminate a step-by-step instruction of our one-pot synthesis protocol, which allows CION to be produced in laboratories with minimal cost.					
34116153	5	54	theme	step-by-step	882:893	arg1	instruction					895:905	a step-by-step instruction	880:905	a step-by-step instruction	880:905	Here we disseminate a step-by-step instruction of our one-pot synthesis protocol, which allows CION to be produced in laboratories with minimal cost.					
34116153	5	55	theme	synthesis	922:930	arg1	protocol					932:939	our one-pot synthesis protocol	910:939	our one-pot synthesis protocol	910:939	Here we disseminate a step-by-step instruction of our one-pot synthesis protocol, which allows CION to be produced in laboratories with minimal cost.					
34116153	7	56	theme	visual	1411:1416	arg1	stimulation					1418:1428	visual stimulation	1411:1428	visual stimulation	1411:1428	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	1	57	theme	Superparamagnetic	125:141	arg1	agents					188:193	robust contrast agents	172:193	robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV)	172:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	57	theme	Superparamagnetic	125:141	arg1	nanoparticles					154:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	6	58	theme	chemical	1161:1168	arg1	composition					1170:1180	chemical composition	1161:1180	chemical composition	1161:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	7	59	theme	several	1246:1252	arg1	experiments					1271:1281	several proof-of-concept experiments	1246:1281	several proof-of-concept experiments	1246:1281	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	3	60	theme	inaccessible	625:636	arg1	methods					610:616	CBV-MRI methods	602:616	CBV-MRI methods either inaccessible or very costly	602:651	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	8	61	theme	cross-modality	1537:1550	arg1	platform					1561:1568	a cross-modality research platform	1535:1568	a cross-modality research platform	1535:1568	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	8	61	theme	cross-modality	1537:1550	arg1	CION					1517:1520	CION	1517:1520	CION	1517:1520	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	7	62	theme	hypercapnic	1384:1394	arg1	challenge					1400:1408	hypercapnic CO2 challenge	1384:1408	hypercapnic CO2 challenge	1384:1408	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	1	63	theme	robust	172:177	arg1	agents					188:193	robust contrast agents	172:193	robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV)	172:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	63	theme	robust	172:177	arg1	nanoparticles					154:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles	125:166	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	4	64	dep	simple	705:710	arg1	one-pot					713:719	one-pot	713:719	one-pot	713:719	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	7	65	theme	optogenetic	1440:1450	arg1	stimulation					1452:1462	targeted optogenetic stimulation	1431:1462	targeted optogenetic stimulation	1431:1462	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	4	66	theme	Oxide	776:780	arg1	Nanoparticles					782:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	6	67	from	shifts	1187:1192	arg1	pharmacokinetics					1215:1230	pharmacokinetics	1215:1230	pharmacokinetics	1215:1230	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	6	67	from	shifts	1187:1192	arg1	relaxivity					1200:1209	MR relaxivity	1197:1209	MR relaxivity	1197:1209	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	0	68	theme	MRA	107:109	arg1	applications					111:122	preclinical fMRI and MRA applications	86:122	applications	111:122	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	8	69	theme	in	1592:1593	arg1	optical					1600:1606	concurrent in vivo optical	1581:1606	concurrent in vivo optical	1581:1606	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	6	70	theme	stoichiometric	1093:1106	arg1	ratio					1108:1112	metal stoichiometric ratio	1087:1112	metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition	1087:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	0	71	theme	carboxymethyl-dextran	21:41	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of carboxymethyl-dextran	0:41	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	1	72	theme	cerebral	292:299	arg1	CBV					315:317	CBV	315:317	CBV	315:317	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	1	72	theme	cerebral	292:299	arg1	volume					307:312	the cerebral blood volume	288:312	the cerebral blood volume (CBV)	288:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	7	73	theme	functional	1330:1339	arg1	stimulation					1452:1462	targeted optogenetic stimulation	1431:1462	targeted optogenetic stimulation	1431:1462	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	73	theme	functional	1330:1339	arg1	microangiography					1469:1484	microangiography	1469:1484	microangiography	1469:1484	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	73	theme	functional	1330:1339	arg1	challenge					1400:1408	hypercapnic CO2 challenge	1384:1408	hypercapnic CO2 challenge	1384:1408	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	73	theme	functional	1330:1339	arg1	applications					1360:1371	functional and structural MRI applications	1330:1371	functional and structural MRI applications	1330:1371	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	7	73	theme	functional	1330:1339	arg1	stimulation					1418:1428	visual stimulation	1411:1428	visual stimulation	1411:1428	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	8	74	theme	MRI	1612:1614	arg1	measurement					1616:1626	MRI measurement	1612:1626	MRI measurement	1612:1626	We also present evidence that CION can serve as a cross-modality research platform by showing concurrent in vivo optical and MRI measurement of CBV using fluorescent-labeled CION.					
34116153	4	75	theme	coated	764:769	arg1	Nanoparticles					782:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	Carboxymethyl-dextran coated Iron Oxide Nanoparticles	742:794	To address this barrier, we developed a simple, one-pot recipe to synthesize Carboxymethyl-dextran coated Iron Oxide Nanoparticles, namely, "CION", suitable for preclinical CBV-MRI applications.					
34116153	0	76	theme	iron	50:53	arg1	CION					76:79	CION	76:79	CION	76:79	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	0	76	theme	iron	50:53	arg1	nanoparticles					61:73	iron oxide nanoparticles	50:73	iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications	50:122	One-pot synthesis of carboxymethyl-dextran coated iron oxide nanoparticles (CION) for preclinical fMRI and MRA applications.					
34116153	1	77	theme	volume	307:312	arg1	mapping					277:283	sensitive structural and functional mapping	241:283	sensitive structural and functional mapping of the cerebral blood volume (CBV)	241:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
34116153	7	78	theme	CION	1321:1324	arg1	utility					1310:1316	the utility	1306:1316	the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography	1306:1484	We performed several proof-of-concept experiments in vivo, demonstrating the utility of CION for functional and structural MRI applications, including hypercapnic CO2 challenge, visual stimulation, targeted optogenetic stimulation, and microangiography.					
34116153	2	79	theme	iron-deficiency	455:469	arg1	treatment					442:450	the clinical treatment	429:450	the clinical treatment of iron-deficiency	429:469	To date, many CBV-MRI studies are conducted with Feraheme, manufactured for the clinical treatment of iron-deficiency.					
34116153	5	80	theme	minimal	996:1002	arg1	cost					1004:1007	minimal cost	996:1007	minimal cost	996:1007	Here we disseminate a step-by-step instruction of our one-pot synthesis protocol, which allows CION to be produced in laboratories with minimal cost.					
34116153	6	81	theme	surface	1138:1144	arg1	chemistry					1146:1154	surface chemistry	1138:1154	surface chemistry	1138:1154	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	2	82	theme	many	362:365	arg1	studies					375:381	many CBV-MRI studies	362:381	many CBV-MRI studies	362:381	To date, many CBV-MRI studies are conducted with Feraheme, manufactured for the clinical treatment of iron-deficiency.					
34116153	6	83	theme	size	1132:1135	arg1	terms					1117:1121	terms	1117:1121	terms of their size, surface chemistry, and chemical composition	1117:1180	We also characterized different CION-conjugations by manipulating polymer to metal stoichiometric ratio in terms of their size, surface chemistry, and chemical composition, and shifts in MR relaxivity and pharmacokinetics.					
34116153	3	84	theme	United	535:540	arg1	States					542:547	the United States	531:547	the United States due to commercial and regulatory constraints	531:592	Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making CBV-MRI methods either inaccessible or very costly to achieve.					
34116153	1	85	theme	structural	251:260	arg1	mapping					277:283	sensitive structural and functional mapping	241:283	sensitive structural and functional mapping of the cerebral blood volume (CBV)	241:318	Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) used for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously.					
33446921	2	0	from	countries	390:398	arg1	infants					362:368	their infants	356:368	their infants from seven European countries	356:398	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	2	0	from	countries	390:398	arg1	women					346:350	370 healthy, pregnant women	324:350	370 healthy, pregnant women	324:350	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	10	1	theme	growth	1499:1504	arg1	outcomes					1506:1513	infant growth outcomes	1492:1513	infant growth outcomes	1492:1513	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	11	2	theme	associations	1608:1619	arg1	investigation					1591:1603	a comprehensive investigation	1575:1603	a comprehensive investigation of associations between all major HMO	1575:1641	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	11	2	theme	associations	1608:1619	arg1	adiposity					1665:1673	adiposity	1665:1673	adiposity	1665:1673	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	11	2	theme	associations	1608:1619	arg1	growth					1654:1659	infant growth	1647:1659	infant growth	1647:1659	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	2	3	theme	healthy	328:334	arg1	women					346:350	370 healthy, pregnant women	324:350	370 healthy, pregnant women	324:350	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	1	4	theme	comprehensive	227:239	arg1	studies					241:247	comprehensive studies	227:247	comprehensive studies	227:247	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	8	5	theme	significant	1094:1104	arg1	associations					1106:1117	No significant associations	1091:1117	No significant associations between growth and body composition	1091:1153	No significant associations between growth and body composition and Lewis or secretor-dependent HMOs like 2'-fucosyllactose were identified.					
33446921	10	6	theme	growing	1535:1541	arg1	populations					1543:1553	healthy growing populations	1527:1553	healthy growing populations	1527:1553	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	11	7	theme	infant	1647:1652	arg1	growth					1654:1659	infant growth	1647:1659	infant growth	1647:1659	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	7	8	theme	gain	1058:1061	arg1	percentile					1014:1023	the lower 25th percentile	999:1023	the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation	999:1088	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	7	9	theme	weight-for-length	1032:1048	arg1	gain					1058:1061	the weight-for-length z-score gain	1028:1061	the weight-for-length z-score gain over 4 months of lactation	1028:1088	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	6	10	theme	Sialyllacto-N-tetraose	754:775	arg1	LSTc					780:783	LSTc	780:783	LSTc	780:783	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	6	10	theme	Sialyllacto-N-tetraose	754:775	arg1	c					777:777	Sialyllacto-N-tetraose c	754:777	Sialyllacto-N-tetraose c (LSTc)	754:784	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	3	11	theme	infant	507:512	arg1	parameters					514:523	infant parameters	507:523	infant parameters	507:523	Breastmilk samples were collected using standardized procedures at six time points over 4 months, as were infant parameters.					
33446921	14	12	theme	Future	1877:1882	arg1	studies					1895:1901	Future population studies	1877:1901	Future population studies	1877:1901	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	9	13	theme	mothers	1411:1417	arg1	cohort					1393:1398	this cohort	1388:1398	this cohort of healthy mothers and infants	1388:1429	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	5	14	dep	rs = -0.18	731:740	arg1	P = 0.02					743:750	P = 0.02	743:750	P = 0.02	743:750	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	12	15	theme	HMOs	1724:1727	arg1	HMOs					1724:1727	HMOs	1724:1727	HMOs	1724:1727	Certain groups of HMOs, like the sialylated, may be associated with adiposity during the first months of lactation.					
33446921	12	15	theme	HMOs	1724:1727	arg1	groups					1714:1719	Certain groups	1706:1719	Certain groups	1706:1719	Certain groups of HMOs, like the sialylated, may be associated with adiposity during the first months of lactation.					
33446921	7	16	theme	25th	1009:1012	arg1	percentile					1014:1023	the lower 25th percentile	999:1023	the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation	999:1088	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	5	17	from	change	713:718	arg1	length					723:728	length	723:728	length	723:728	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	9	18	theme	infants	1423:1429	arg1	cohort					1393:1398	this cohort	1388:1398	this cohort of healthy mothers and infants	1388:1429	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	8	19	theme	Lewis	1159:1163	arg1	HMOs					1187:1190	Lewis or secretor-dependent HMOs	1159:1190	HMOs	1187:1190	No significant associations between growth and body composition and Lewis or secretor-dependent HMOs like 2'-fucosyllactose were identified.					
33446921	14	20	from	role	1923:1926	arg1	context					1962:1968	the context	1958:1968	the context of health and disease	1958:1990	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	8	21	theme	secretor-dependent	1168:1185	arg1	HMOs					1187:1190	Lewis or secretor-dependent HMOs	1159:1190	HMOs	1187:1190	No significant associations between growth and body composition and Lewis or secretor-dependent HMOs like 2'-fucosyllactose were identified.					
33446921	7	22	theme	z-score	1050:1056	arg1	gain					1058:1061	the weight-for-length z-score gain	1028:1061	the weight-for-length z-score gain over 4 months of lactation	1028:1088	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	9	23	theme	CONCLUSIONS	1232:1242	arg1	Changes					1244:1250	CONCLUSIONS Changes	1232:1250	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months	1232:1313	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	12	24	theme	Certain	1706:1712	arg1	HMOs					1724:1727	HMOs	1724:1727	HMOs	1724:1727	Certain groups of HMOs, like the sialylated, may be associated with adiposity during the first months of lactation.					
33446921	12	24	theme	Certain	1706:1712	arg1	groups					1714:1719	Certain groups	1706:1719	Certain groups	1706:1719	Certain groups of HMOs, like the sialylated, may be associated with adiposity during the first months of lactation.					
33446921	6	25	contain	had	786:788	arg1	LSTc					780:783	LSTc	780:783	LSTc	780:783	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	6	25	contain	had	786:788	arg2	correlation					801:811	a positive correlation	790:811	a positive correlation with weight for length (rs = 0.19, P = 0.015)	790:857	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	6	25	contain	had	786:788	arg1	c					777:777	Sialyllacto-N-tetraose c	754:777	Sialyllacto-N-tetraose c (LSTc)	754:784	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	7	26	dep	higher	911:916	arg1	P = 0.017					948:956	P = 0.017	948:956	P = 0.017	948:956	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	7	26	dep	higher	911:916	arg1	P = 0.006					962:970	P = 0.006	962:970	P = 0.006	962:970	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	1	27	theme	human	132:136	arg1	HMOs					161:164	HMOs	161:164	HMOs	161:164	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	1	27	theme	human	132:136	arg1	oligosaccharides					143:158	human milk oligosaccharides	132:158	human milk oligosaccharides (HMOs)	132:165	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	5	28	dep	had	677:679	arg1	rs = -0.18					731:740	rs = -0.18	731:740	rs = -0.18	731:740	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	9	29	theme	HMO	1259:1261	arg1	composition					1263:1273	the HMO composition	1255:1273	the HMO composition of breastmilk	1255:1287	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	13	30	theme	metabolic	1858:1866	arg1	disease					1868:1874	future metabolic disease	1851:1874	future metabolic disease	1851:1874	HMO may modulate the risk of future metabolic disease.					
33446921	7	31	from	percentile	886:895	arg1	Infants					860:866	Infants	860:866	Infants at the 25th upper percentile	860:895	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	10	32	theme	IMPACT	1432:1437	arg1	associations					1446:1457	IMPACT Modest associations	1432:1457	IMPACT Modest associations	1432:1457	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	7	33	theme	upper	880:884	arg1	percentile					886:895	the 25th upper percentile	871:895	the 25th upper percentile	871:895	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	3	34	theme	Breastmilk	401:410	arg1	samples					412:418	Breastmilk samples	401:418	Breastmilk samples	401:418	Breastmilk samples were collected using standardized procedures at six time points over 4 months, as were infant parameters.					
33446921	0	35	theme	Human	0:4	arg1	oligosaccharides					11:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides, infant growth, and adiposity over the first 4 months of lactation.					
33446921	9	36	theme	first	1300:1304	arg1	months					1308:1313	the first 4 months	1296:1313	the first 4 months	1296:1313	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	14	37	theme	HMOs	1950:1953	arg1	HMOs					1950:1953	HMOs	1950:1953	HMOs	1950:1953	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	14	37	theme	HMOs	1950:1953	arg1	groups					1940:1945	specific groups	1931:1945	specific groups of HMOs	1931:1953	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	14	38	theme	groups	1940:1945	arg1	role					1923:1926	the role	1919:1926	the role of specific groups of HMOs in the context of health and disease	1919:1990	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	5	39	with	correlation	692:702	arg1	change					713:718	the change	709:718	the change in length	709:728	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	5	40	contain	had	677:679	arg2	correlation					692:702	a negative correlation	681:702	a negative correlation with the change in length	681:728	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	5	40	contain	had	677:679	arg1	Lacto-N-neotetraose					657:675	RESULTS Lacto-N-neotetraose	649:675	RESULTS Lacto-N-neotetraose	649:675	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	14	41	theme	long-term	2010:2018	arg1	impact					2020:2025	the long-term impact	2006:2025	the long-term impact	2006:2025	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	11	42	theme	several	1685:1691	arg1	points					1698:1703	several time points	1685:1703	several time points	1685:1703	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	2	43	theme	European	381:388	arg1	countries					390:398	seven European countries	375:398	seven European countries	375:398	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	0	44	theme	infant	29:34	arg1	growth					36:41	infant growth	29:41	infant growth	29:41	Human milk oligosaccharides, infant growth, and adiposity over the first 4 months of lactation.					
33446921	10	45	theme	infant	1492:1497	arg1	outcomes					1506:1513	infant growth outcomes	1492:1513	infant growth outcomes	1492:1513	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	9	46	theme	little	1330:1335	arg1	influence					1337:1345	little influence	1330:1345	little influence	1330:1345	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	12	47	theme	first	1795:1799	arg1	months					1801:1806	the first months	1791:1806	the first months of lactation	1791:1819	Certain groups of HMOs, like the sialylated, may be associated with adiposity during the first months of lactation.					
33446921	14	48	theme	disease	1984:1990	arg1	context					1962:1968	the context	1958:1968	the context of health and disease	1958:1990	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	12	49	theme	lactation	1811:1819	arg1	months					1801:1806	the first months	1791:1806	the first months of lactation	1791:1819	Certain groups of HMOs, like the sialylated, may be associated with adiposity during the first months of lactation.					
33446921	0	50	dep	oligosaccharides	11:26	arg1	months					75:80	the first 4 months	63:80	the first 4 months of lactation	63:93	Human milk oligosaccharides, infant growth, and adiposity over the first 4 months of lactation.					
33446921	9	51	from	Changes	1244:1250	arg1	composition					1263:1273	the HMO composition	1255:1273	the HMO composition of breastmilk	1255:1287	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	4	52	theme	anthropometric	590:603	arg1	data					605:608	anthropometric data	590:608	anthropometric data	590:608	Correlations and associations between HMO area under the curve, anthropometric data, and fat mass at 4 months were tested.					
33446921	11	53	theme	major	1633:1637	arg1	HMO					1639:1641	all major HMO	1629:1641	all major HMO	1629:1641	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	11	54	theme	comprehensive	1577:1589	arg1	investigation					1591:1603	a comprehensive investigation	1575:1603	a comprehensive investigation of associations between all major HMO	1575:1641	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	9	55	theme	body	1368:1371	arg1	composition					1373:1383	body composition	1368:1383	body composition	1368:1383	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	4	56	theme	HMO	564:566	arg1	area					568:571	HMO area	564:571	HMO area under the curve	564:587	Correlations and associations between HMO area under the curve, anthropometric data, and fat mass at 4 months were tested.					
33446921	1	57	from	literature	278:287	arg1	missing					253:259	missing	253:259	missing	253:259	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	10	58	theme	healthy	1527:1533	arg1	populations					1543:1553	healthy growing populations	1527:1553	healthy growing populations	1527:1553	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	4	59	from	months	629:634	arg1	associations					543:554	associations	543:554	associations	543:554	Correlations and associations between HMO area under the curve, anthropometric data, and fat mass at 4 months were tested.					
33446921	4	59	from	months	629:634	arg1	Correlations					526:537	Correlations	526:537	Correlations	526:537	Correlations and associations between HMO area under the curve, anthropometric data, and fat mass at 4 months were tested.					
33446921	14	60	theme	population	1884:1893	arg1	studies					1895:1901	Future population studies	1877:1901	Future population studies	1877:1901	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	9	61	theme	healthy	1403:1409	arg1	mothers					1411:1417	healthy mothers	1403:1417	healthy mothers	1403:1417	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	7	62	theme	lower	1003:1007	arg1	percentile					1014:1023	the lower 25th percentile	999:1023	the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation	999:1088	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	1	63	theme	infant	171:176	arg1	growth					178:183	infant growth	171:183	infant growth	171:183	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	8	64	theme	body	1138:1141	arg1	composition					1143:1153	body composition	1138:1153	body composition	1138:1153	No significant associations between growth and body composition and Lewis or secretor-dependent HMOs like 2'-fucosyllactose were identified.					
33446921	5	65	theme	negative	683:690	arg1	correlation					692:702	a negative correlation	681:702	a negative correlation with the change in length	681:728	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	4	66	theme	fat	615:617	arg1	mass					619:622	fat mass	615:622	fat mass	615:622	Correlations and associations between HMO area under the curve, anthropometric data, and fat mass at 4 months were tested.					
33446921	13	67	theme	disease	1868:1874	arg1	risk					1843:1846	the risk	1839:1846	the risk of future metabolic disease	1839:1874	HMO may modulate the risk of future metabolic disease.					
33446921	5	68	theme	RESULTS	649:655	arg1	Lacto-N-neotetraose					657:675	RESULTS Lacto-N-neotetraose	649:675	RESULTS Lacto-N-neotetraose	649:675	RESULTS Lacto-N-neotetraose had a negative correlation with the change in length (rs = -0.18, P = 0.02).					
33446921	3	69	dep	parameters	514:523	arg1	were					502:505	were	502:505	were	502:505	Breastmilk samples were collected using standardized procedures at six time points over 4 months, as were infant parameters.					
33446921	3	69	dep	parameters	514:523	arg1	as					499:500	as	499:500	as	499:500	Breastmilk samples were collected using standardized procedures at six time points over 4 months, as were infant parameters.					
33446921	2	70	dep	METHODS	290:296	arg1	recruited					314:322	recruited	314:322	recruited 370 healthy, pregnant women and their infants from seven European countries	314:398	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	2	70	dep	METHODS	290:296	arg1	screened					301:308	screened	301:308	screened	301:308	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	0	71	theme	first	67:71	arg1	months					75:80	the first 4 months	63:80	the first 4 months of lactation	63:93	Human milk oligosaccharides, infant growth, and adiposity over the first 4 months of lactation.					
33446921	1	72	theme	milk	138:141	arg1	HMOs					161:164	HMOs	161:164	HMOs	161:164	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	1	72	theme	milk	138:141	arg1	oligosaccharides					143:158	human milk oligosaccharides	132:158	human milk oligosaccharides (HMOs)	132:165	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	13	73	theme	future	1851:1856	arg1	disease					1868:1874	future metabolic disease	1851:1874	future metabolic disease	1851:1874	HMO may modulate the risk of future metabolic disease.					
33446921	7	74	theme	higher	911:916	arg1	milk					906:909	milk	906:909	milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively)	906:985	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	14	75	theme	health	1973:1978	arg1	context					1962:1968	the context	1958:1968	the context of health and disease	1958:1990	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	2	76	dep	healthy	328:334	arg1	pregnant					337:344	pregnant	337:344	pregnant	337:344	METHODS We screened and recruited 370 healthy, pregnant women and their infants from seven European countries.					
33446921	0	77	theme	lactation	85:93	arg1	months					75:80	the first 4 months	63:80	the first 4 months of lactation	63:93	Human milk oligosaccharides, infant growth, and adiposity over the first 4 months of lactation.					
33446921	9	78	theme	breastmilk	1278:1287	arg1	composition					1263:1273	the HMO composition	1255:1273	the HMO composition of breastmilk	1255:1287	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	1	79	dep	BACKGROUND	96:105	arg1	missing					253:259	missing	253:259	missing	253:259	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	1	79	dep	BACKGROUND	96:105	arg1	understood					212:221	understood	212:221	is not fully understood	199:221	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	0	80	theme	milk	6:9	arg1	oligosaccharides					11:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides, infant growth, and adiposity over the first 4 months of lactation.					
33446921	7	81	theme	25th	875:878	arg1	percentile					886:895	the 25th upper percentile	871:895	the 25th upper percentile	871:895	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	7	82	theme	lactation	1080:1088	arg1	months					1070:1075	4 months	1068:1075	4 months of lactation	1068:1088	Infants at the 25th upper percentile were fed milk higher in 3'-sialyllactose and LSTc (P = 0.017 and P = 0.006, respectively) compared to the lower 25th percentile of the weight-for-length z-score gain over 4 months of lactation.					
33446921	1	83	theme	current	270:276	arg1	literature					278:287	the current literature	266:287	the current literature	266:287	BACKGROUND The relationship between human milk oligosaccharides (HMOs) and infant growth and adiposity is not fully understood and comprehensive studies are missing from the current literature.					
33446921	14	84	theme	specific	1931:1938	arg1	HMOs					1950:1953	HMOs	1950:1953	HMOs	1950:1953	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	14	84	theme	specific	1931:1938	arg1	groups					1940:1945	specific groups	1931:1945	specific groups of HMOs	1931:1953	Future population studies need to address the role of specific groups of HMOs in the context of health and disease to understand the long-term impact.					
33446921	6	85	with	correlation	801:811	arg1	weight					818:823	weight	818:823	weight for length (rs = 0.19, P = 0.015)	818:857	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	10	86	theme	Modest	1439:1444	arg1	associations					1446:1457	IMPACT Modest associations	1432:1457	IMPACT Modest associations	1432:1457	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	3	87	theme	standardized	441:452	arg1	procedures					454:463	standardized procedures	441:463	standardized procedures	441:463	Breastmilk samples were collected using standardized procedures at six time points over 4 months, as were infant parameters.					
33446921	6	88	dep	length	829:834	arg1	P = 0.015					848:856	P = 0.015	848:856	P = 0.015	848:856	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	6	88	dep	length	829:834	arg1	rs = 0.19					837:845	rs = 0.19	837:845	rs = 0.19	837:845	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	6	89	theme	positive	792:799	arg1	correlation					801:811	a positive correlation	790:811	a positive correlation with weight for length (rs = 0.19, P = 0.015)	790:857	Sialyllacto-N-tetraose c (LSTc) had a positive correlation with weight for length (rs = 0.19, P = 0.015).					
33446921	10	90	theme	individual	1473:1482	arg1	HMO					1484:1486	individual HMO	1473:1486	individual HMO	1473:1486	IMPACT Modest associations exist between individual HMO and infant growth outcomes at least in healthy growing populations.					
33446921	11	91	theme	time	1693:1696	arg1	points					1698:1703	several time points	1685:1703	several time points	1685:1703	Our study provides a comprehensive investigation of associations between all major HMO and infant growth and adiposity including several time points.					
33446921	3	92	theme	time	472:475	arg1	points					477:482	six time points	468:482	six time points over 4 months	468:496	Breastmilk samples were collected using standardized procedures at six time points over 4 months, as were infant parameters.					
33446921	9	93	theme	infant	1350:1355	arg1	growth					1357:1362	infant growth	1350:1362	infant growth	1350:1362	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	9	94	contain	have	1325:1328	arg2	influence					1337:1345	little influence	1330:1345	little influence	1330:1345	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33446921	9	94	contain	have	1325:1328	arg1	Changes					1244:1250	CONCLUSIONS Changes	1232:1250	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months	1232:1313	CONCLUSIONS Changes in the HMO composition of breastmilk during the first 4 months appear to have little influence on infant growth and body composition in this cohort of healthy mothers and infants.					
33515990	2	0	from	effects	359:365	arg1	metabolism					440:449	nutrient metabolism	431:449	nutrient metabolism	431:449	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	2	0	from	effects	359:365	arg1	function					459:466	gut function	455:466	gut function	455:466	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	12	1	contain	having	1965:1970	arg1	22F					1961:1963	22F	1961:1963	22F having the highest values	1961:1989	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	12	1	contain	having	1965:1970	arg2	values					1984:1989	the highest values	1972:1989	the highest values	1972:1989	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	12	2	theme	highest	1976:1982	arg1	values					1984:1989	the highest values	1972:1989	the highest values	1972:1989	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	4	3	dep	18F	918:920	arg1	fat					1006:1008	fat	1006:1008	fat	1006:1008	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	3	dep	18F	918:920	arg1	%					927:927	18.8%	923:927	18.8%	923:927	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	3	dep	18F	918:920	arg1	%					937:937	47.6%	933:937	47.6%	933:937	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	3	dep	18F	918:920	arg1	DM					1022:1023	lactose DM	1014:1023	lactose DM	1014:1023	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	3	4	from	effect	571:576	arg1	performance					632:642	growth performance	625:642	growth performance	625:642	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	3	4	from	effect	571:576	arg1	digestibility					654:666	nutrient digestibility and metabolism	645:681	digestibility	654:666	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	3	4	from	effect	571:576	arg1	sensitivity					704:714	glucose/insulin sensitivity	688:714	glucose/insulin sensitivity	688:714	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	14	5	theme	biological	2471:2480	arg1	impact					2482:2487	biological impact	2471:2487	biological impact of fat and lactose levels in CMR for rearing calves	2471:2539	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	5	6	theme	teat	1181:1184	arg1	bucket					1186:1191	a teat bucket	1179:1191	a teat bucket	1179:1191	Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket.					
33515990	1	7	theme	milk	249:252	arg1	formulations					269:280	calf milk replacer (CMR) formulations	244:280	calf milk replacer (CMR) formulations	244:280	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	4	8	theme	46.7 ± 0.76 kg	768:781	arg1	BW					783:784	46.7 ± 0.76 kg BW	768:784	46.7 ± 0.76 kg BW	768:784	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	2	9	from	ratio	299:303	arg1	CMR					308:310	CMR	308:310	CMR	308:310	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	14	10	theme	rearing	2526:2532	arg1	calves					2534:2539	rearing calves	2526:2539	rearing calves	2526:2539	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	6	11	from	56	1320:1321	arg1	weaning					1307:1313	gradual weaning	1299:1313	gradual weaning from 56 to 63 days	1299:1332	The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days.					
33515990	12	12	dep	plasma	1879:1884	arg1	differences					1933:1943	differences	1933:1943	differences	1933:1943	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	12	12	dep	plasma	1879:1884	arg1	acids					1907:1911	non-esterified fatty acids	1886:1911	non-esterified fatty acids	1886:1911	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	14	13	theme	adult	2446:2450	arg1	life					2452:2455	adult life	2446:2455	adult life	2446:2455	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	1	14	theme	elevated	168:175	arg1	planes					177:182	elevated planes	168:182	elevated planes of milk feeding of young calves	168:214	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	0	15	theme	dairy	107:111	arg1	calves					113:118	dairy calves	107:118	dairy calves	107:118	Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves.					
33515990	2	16	theme	rearing	471:477	arg1	calves					479:484	rearing calves	471:484	rearing calves	471:484	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	14	17	theme	minor	2325:2329	arg1	effects					2331:2337	minor effects	2325:2337	minor effects on nutrient metabolism	2325:2360	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	9	18	theme	age	1605:1607	arg1	28					1587:1588	28	1587:1588	28	1587:1588	A glucose tolerance test (GTT) was performed between 28 and 32 days of age.					
33515990	9	18	theme	age	1605:1607	arg1	age					1605:1607	age	1605:1607	age	1605:1607	A glucose tolerance test (GTT) was performed between 28 and 32 days of age.					
33515990	3	19	theme	current	534:540	arg1	study					542:546	the current study	530:546	the current study	530:546	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	1	20	theme	feeding	192:198	arg1	planes					177:182	elevated planes	168:182	elevated planes of milk feeding of young calves	168:214	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	10	21	theme	energy	1635:1640	arg1	intake					1642:1647	metabolizable energy intake	1621:1647	metabolizable energy intake from CMR	1621:1656	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	4	22	dep	fat	901:903	arg1	18F					918:920	18F	918:920	18F	918:920	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	8	23	theme	blood	1516:1520	arg1	parameters					1522:1531	blood parameters	1516:1531	blood parameters	1516:1531	Measurements included feed intake, growth, nutrient digestibility, fecal composition, and blood parameters.					
33515990	12	24	theme	total	1917:1921	arg1	bilirubin					1923:1931	total bilirubin	1917:1931	total bilirubin	1917:1931	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	14	25	theme	growth	2293:2298	arg1	performance					2300:2310	growth performance	2293:2310	growth performance	2293:2310	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	2	26	theme	nutrient	431:438	arg1	metabolism					440:449	nutrient metabolism	431:449	nutrient metabolism	431:449	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	0	27	theme	lactose	58:64	arg1	replacement					43:53	the replacement	39:53	the replacement of lactose by fat	39:71	Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves.					
33515990	14	28	from	impact	2482:2487	arg1	CMR					2518:2520	CMR	2518:2520	CMR	2518:2520	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	8	29	theme	nutrient	1469:1476	arg1	digestibility					1478:1490	nutrient digestibility	1469:1490	nutrient digestibility	1469:1490	Measurements included feed intake, growth, nutrient digestibility, fecal composition, and blood parameters.					
33515990	7	30	theme	ad	1393:1394	arg1	libitum					1396:1402	available ad libitum	1383:1402	available ad libitum	1383:1402	Pelleted starter, chopped straw, and water were available ad libitum throughout the study.					
33515990	11	31	theme	higher	1830:1835	arg1	content					1841:1847	higher fat content	1830:1847	higher fat content in 22F compared to 26F	1830:1870	Fecal composition remained unaffected by treatment except for higher fat content in 22F compared to 26F.					
33515990	12	32	theme	non-esterified	1886:1899	arg1	acids					1907:1911	non-esterified fatty acids	1886:1911	non-esterified fatty acids	1886:1911	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	4	33	theme	treatments	859:868	arg1	one					847:849	one	847:849	one	847:849	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	33	theme	treatments	859:868	arg1	treatments					859:868	four treatments	854:868	four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	854:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	34	theme	lactose	1014:1020	arg1	DM					1022:1023	lactose DM	1014:1023	lactose DM	1014:1023	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	34	theme	lactose	1014:1020	arg1	%					927:927	18.8%	923:927	18.8%	923:927	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	5	35	dep	day	1152:1154	arg1	1600 h					1166:1171	1600 h	1166:1171	1600 h	1166:1171	Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket.					
33515990	5	35	dep	day	1152:1154	arg1	0700					1157:1160	0700	1157:1160	0700	1157:1160	Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket.					
33515990	4	36	theme	differing	881:889	arg1	levels					891:896	differing levels	881:896	differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	881:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	2	37	theme	whole	344:348	arg1	milk					350:353	whole milk	344:353	whole milk	344:353	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	0	38	theme	Metabolic	0:8	arg1	responses					26:34	Metabolic and performance responses	0:34	Metabolic and performance responses to the replacement of lactose by fat in milk	0:79	Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves.					
33515990	1	39	theme	replacer	254:261	arg1	formulations					269:280	calf milk replacer (CMR) formulations	244:280	calf milk replacer (CMR) formulations	244:280	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	6	40	theme	gradual	1299:1305	arg1	weaning					1307:1313	gradual weaning	1299:1313	gradual weaning from 56 to 63 days	1299:1332	The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days.					
33515990	14	41	theme	fat	2261:2263	arg1	ratio					2265:2269	the lactose:fat ratio	2249:2269	altering the lactose:fat ratio in CMR	2240:2276	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	2	42	theme	fat	381:383	arg1	inclusion					385:393	fat inclusion	381:393	fat inclusion	381:393	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	12	43	contain	had	2003:2005	arg2	values					2018:2023	the lowest values	2007:2023	the lowest values	2007:2023	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	12	43	contain	had	2003:2005	arg1	26F					1999:2001	26F	1999:2001	26F	1999:2001	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	0	44	theme	performance	14:24	arg1	responses					26:34	Metabolic and performance responses	0:34	Metabolic and performance responses to the replacement of lactose by fat in milk	0:79	Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves.					
33515990	1	45	theme	CMR	264:266	arg1	formulations					269:280	calf milk replacer (CMR) formulations	244:280	calf milk replacer (CMR) formulations	244:280	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	10	46	from	CMR	1654:1656	arg1	intake					1642:1647	metabolizable energy intake	1621:1647	metabolizable energy intake from CMR	1621:1656	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	7	47	theme	Pelleted	1335:1342	arg1	starter					1344:1350	starter	1344:1350	starter	1344:1350	Pelleted starter, chopped straw, and water were available ad libitum throughout the study.					
33515990	14	48	theme	lactose	2253:2259	arg1	ratio					2265:2269	the lactose:fat ratio	2249:2269	altering the lactose:fat ratio in CMR	2240:2276	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	2	49	theme	lactose	413:419	arg1	expense					402:408	the expense	398:408	the expense of lactose in CMR	398:426	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	3	50	theme	increasing	581:590	arg1	replacement					592:602	increasing replacement	581:602	increasing replacement of lactose by fat	581:620	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	5	51	theme	11 week	1097:1103	arg1	study					1105:1109	the 11 week study	1093:1109	the 11 week study	1093:1109	Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket.					
33515990	14	52	theme	levels	2508:2513	arg1	impact					2482:2487	biological impact	2471:2487	biological impact of fat and lactose levels in CMR for rearing calves	2471:2539	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	1	53	theme	formulations	269:280	arg1	reconsideration					225:239	reconsideration	225:239	reconsideration of calf milk replacer (CMR) formulations	225:280	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	4	54	contain	containing	870:879	arg1	treatments					859:868	four treatments	854:868	four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	854:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	54	contain	containing	870:879	arg2	levels					891:896	differing levels	881:896	differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	881:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	14	55	theme	altering	2240:2247	arg1	ratio					2265:2269	the lactose:fat ratio	2249:2269	altering the lactose:fat ratio in CMR	2240:2276	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	4	56	theme	age	763:765	arg1	1.7 ± 0.10 days					744:758	1.7 ± 0.10 days	744:758	1.7 ± 0.10 days of age	744:765	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	56	theme	age	763:765	arg1	calves					736:741	40 male calves	728:741	40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW)	728:785	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	12	57	theme	fatty	1901:1905	arg1	acids					1907:1911	non-esterified fatty acids	1886:1911	non-esterified fatty acids	1886:1911	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	2	58	theme	lactose	291:297	arg1	ratio					299:303	The fat:lactose ratio	283:303	The fat:lactose ratio in CMR	283:310	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	2	58	theme	lactose	291:297	arg1	lower					325:329	lower	325:329	lower	325:329	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	9	59	theme	glucose	1536:1542	arg1	GTT					1560:1562	GTT	1560:1562	GTT	1560:1562	A glucose tolerance test (GTT) was performed between 28 and 32 days of age.					
33515990	9	59	theme	glucose	1536:1542	arg1	test					1554:1557	A glucose tolerance test	1534:1557	A glucose tolerance test (GTT)	1534:1563	A glucose tolerance test (GTT) was performed between 28 and 32 days of age.					
33515990	4	60	theme	calves	736:741	arg1	total					719:723	A total	717:723	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW)	717:785	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	1	61	theme	calves	209:214	arg1	feeding					192:198	milk feeding	187:198	milk feeding of young calves	187:214	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	14	62	theme	lactose	2500:2506	arg1	levels					2508:2513	fat and lactose levels	2492:2513	fat and lactose levels in CMR	2492:2520	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	5	63	from	bucket	1186:1191	arg1	day					1152:1154	a day	1150:1154	a day (0700 and 1600 h) from a teat bucket	1150:1191	Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket.					
33515990	14	64	theme	fat	2492:2494	arg1	levels					2508:2513	fat and lactose levels	2492:2513	fat and lactose levels in CMR	2492:2520	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	6	65	theme	CMR	1198:1200	arg1	schedule					1210:1217	The CMR feeding schedule	1194:1217	The CMR feeding schedule	1194:1217	The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days.					
33515990	6	66	dep	d	1243:1243	arg1	to					1247:1248	to	1247:1248	to	1247:1248	The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days.					
33515990	1	67	theme	calf	244:247	arg1	formulations					269:280	calf milk replacer (CMR) formulations	244:280	calf milk replacer (CMR) formulations	244:280	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	14	68	theme	future	2367:2372	arg1	investigation					2374:2386	future investigation	2367:2386	future investigation	2367:2386	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	1	69	theme	dairy	145:149	arg1	industry					151:158	the dairy industry	141:158	the dairy industry	141:158	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	3	70	theme	nutrient	645:652	arg1	digestibility					654:666	nutrient digestibility and metabolism	645:681	digestibility	654:666	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	4	71	dep	%	927:927	arg1	%					971:971	26F: 26.0%	962:971	26F: 26.0%	962:971	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	71	dep	%	927:927	arg1	22F					940:942	22F	940:942	22F	940:942	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	71	dep	%	927:927	arg1	%					993:993	30F: 30.1%	984:993	30F: 30.1%	984:993	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	71	dep	%	927:927	arg1	%					959:959	42.8%	955:959	42.8%	955:959	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	71	dep	%	927:927	arg1	%					981:981	38.6%	977:981	38.6%	977:981	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	71	dep	%	927:927	arg1	%					949:949	22.3%	945:949	22.3%	945:949	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	71	dep	%	927:927	arg1	%					1003:1003	33.8%	999:1003	33.8%	999:1003	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	14	72	from	effects	2331:2337	arg1	metabolism					2351:2360	nutrient metabolism	2342:2360	nutrient metabolism	2342:2360	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	2	73	theme	fat	287:289	arg1	ratio					299:303	The fat:lactose ratio	283:303	The fat:lactose ratio in CMR	283:310	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	2	73	theme	fat	287:289	arg1	lower					325:329	lower	325:329	lower	325:329	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	14	74	theme	nutrient	2342:2349	arg1	metabolism					2351:2360	nutrient metabolism	2342:2360	nutrient metabolism	2342:2360	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	3	75	theme	glucose/insulin	688:702	arg1	sensitivity					704:714	glucose/insulin sensitivity	688:714	glucose/insulin sensitivity	688:714	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	11	76	theme	Fecal	1768:1772	arg1	composition					1774:1784	Fecal composition	1768:1784	Fecal composition	1768:1784	Fecal composition remained unaffected by treatment except for higher fat content in 22F compared to 26F.					
33515990	2	77	theme	calves	479:484	arg1	metabolism					440:449	nutrient metabolism	431:449	nutrient metabolism	431:449	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	2	77	theme	calves	479:484	arg1	function					459:466	gut function	455:466	gut function	455:466	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	2	78	from	CMR	424:426	arg1	expense					402:408	the expense	398:408	the expense of lactose in CMR	398:426	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	10	79	theme	concentrate	1748:1758	arg1	intake					1760:1765	concentrate intake	1748:1765	concentrate intake	1748:1765	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	14	80	from	consequences	2418:2429	arg1	life					2452:2455	adult life	2446:2455	adult life	2446:2455	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	1	81	theme	milk	187:190	arg1	feeding					192:198	milk feeding	187:198	milk feeding of young calves	187:214	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	14	82	theme	physiological	2404:2416	arg1	consequences					2418:2429	physiological consequences	2404:2429	physiological consequences	2404:2429	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	14	83	from	effects	2435:2441	arg1	life					2452:2455	adult life	2446:2455	adult life	2446:2455	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	4	84	theme	arrival	809:815	arg1	date					817:820	arrival date	809:820	arrival date	809:820	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	10	85	theme	metabolizable	1621:1633	arg1	intake					1642:1647	metabolizable energy intake	1621:1647	metabolizable energy intake from CMR	1621:1656	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	8	86	theme	feed	1448:1451	arg1	intake					1453:1458	feed intake	1448:1458	feed intake	1448:1458	Measurements included feed intake, growth, nutrient digestibility, fecal composition, and blood parameters.					
33515990	6	87	dep	d	1265:1265	arg1	to					1270:1271	to	1270:1271	to	1270:1271	The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days.					
33515990	1	88	theme	recent	125:130	arg1	trend					132:136	The recent trend	121:136	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves	121:214	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	0	89	from	responses	26:34	arg1	milk					76:79	milk	76:79	milk	76:79	Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves.					
33515990	11	90	theme	fat	1837:1839	arg1	content					1841:1847	higher fat content	1830:1847	higher fat content in 22F compared to 26F	1830:1870	Fecal composition remained unaffected by treatment except for higher fat content in 22F compared to 26F.					
33515990	11	91	from	content	1841:1847	arg1	22F					1852:1854	22F	1852:1854	22F compared to 26F	1852:1870	Fecal composition remained unaffected by treatment except for higher fat content in 22F compared to 26F.					
33515990	7	92	theme	available	1383:1391	arg1	libitum					1396:1402	available ad libitum	1383:1402	available ad libitum	1383:1402	Pelleted starter, chopped straw, and water were available ad libitum throughout the study.					
33515990	2	93	theme	gut	455:457	arg1	function					459:466	gut function	455:466	gut function	455:466	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	2	94	from	expense	402:408	arg1	CMR					424:426	CMR	424:426	CMR	424:426	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	5	95	theme	study	1105:1109	arg1	duration					1081:1088	the duration	1077:1088	the duration of the 11 week study	1077:1109	Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket.					
33515990	10	96	theme	fat	1682:1684	arg1	level					1686:1690	fat level	1682:1690	fat level	1682:1690	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	14	97	from	ratio	2265:2269	arg1	CMR					2274:2276	CMR	2274:2276	CMR	2274:2276	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	8	98	theme	fecal	1493:1497	arg1	composition					1499:1509	fecal composition	1493:1509	fecal composition	1493:1509	Measurements included feed intake, growth, nutrient digestibility, fecal composition, and blood parameters.					
33515990	13	99	theme	total	2045:2049	arg1	area					2051:2054	total area	2045:2054	total area under the curve (AUC) for glucose	2045:2088	Regarding the GTT, total area under the curve (AUC) for glucose was highest in 22F and 26F and lowest in 18F whereas the AUC between 30 and 60 min for glucose was highest in 26F and lowest in 18F and 22F.					
33515990	2	100	from	lactose	413:419	arg1	CMR					424:426	CMR	424:426	CMR	424:426	The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood.					
33515990	14	101	from	CMR	2518:2520	arg1	impact					2482:2487	biological impact	2471:2487	biological impact of fat and lactose levels in CMR for rearing calves	2471:2539	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	1	102	from	trend	132:136	arg1	industry					151:158	the dairy industry	141:158	the dairy industry	141:158	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	7	103	theme	chopped	1353:1359	arg1	straw					1361:1365	chopped straw	1353:1365	chopped straw	1353:1365	Pelleted starter, chopped straw, and water were available ad libitum throughout the study.					
33515990	10	104	from	difference	1722:1731	arg1	ADG					1740:1742	ADG	1740:1742	ADG	1740:1742	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	10	104	from	difference	1722:1731	arg1	BW					1736:1737	BW	1736:1737	BW	1736:1737	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	10	104	from	difference	1722:1731	arg1	intake					1760:1765	concentrate intake	1748:1765	concentrate intake	1748:1765	By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake.					
33515990	4	105	dep	1.7 ± 0.10 days	744:758	arg1	BW					783:784	46.7 ± 0.76 kg BW	768:784	46.7 ± 0.76 kg BW	768:784	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	106	theme	lactose	909:915	arg1	levels					891:896	differing levels	881:896	differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	881:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	107	theme	fat	901:903	arg1	levels					891:896	differing levels	881:896	differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	881:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	108	dep	blocked	792:798	arg1	based					800:804	based	800:804	based on arrival date	800:820	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	108	dep	blocked	792:798	arg1	assigned					835:842	assigned	835:842	randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively)	826:1038	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	3	109	theme	lactose	607:613	arg1	replacement					592:602	increasing replacement	581:602	increasing replacement of lactose by fat	581:620	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	14	110	from	levels	2508:2513	arg1	CMR					2518:2520	CMR	2518:2520	CMR	2518:2520	Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.					
33515990	3	111	theme	replacement	592:602	arg1	effect					571:576	the effect	567:576	the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity	567:714	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	1	112	theme	young	203:207	arg1	calves					209:214	young calves	203:214	young calves	203:214	The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations.					
33515990	9	113	theme	tolerance	1544:1552	arg1	GTT					1560:1562	GTT	1560:1562	GTT	1560:1562	A glucose tolerance test (GTT) was performed between 28 and 32 days of age.					
33515990	9	113	theme	tolerance	1544:1552	arg1	test					1554:1557	A glucose tolerance test	1534:1557	A glucose tolerance test (GTT)	1534:1563	A glucose tolerance test (GTT) was performed between 28 and 32 days of age.					
33515990	12	114	theme	lowest	2011:2016	arg1	values					2018:2023	the lowest values	2007:2023	the lowest values	2007:2023	Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values.					
33515990	3	115	theme	growth	625:630	arg1	performance					632:642	growth performance	625:642	growth performance	625:642	Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity.					
33515990	4	116	theme	male	731:734	arg1	1.7 ± 0.10 days					744:758	1.7 ± 0.10 days	744:758	1.7 ± 0.10 days of age	744:765	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	4	116	theme	male	731:734	arg1	calves					736:741	40 male calves	728:741	40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW)	728:785	A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively).					
33515990	6	117	theme	feeding	1202:1208	arg1	schedule					1210:1217	The CMR feeding schedule	1194:1217	The CMR feeding schedule	1194:1217	The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days.					
33772084	0	0	theme	metabolism	86:95	arg1	modulation					53:62	modulation	53:62	modulation of gut microbiota and metabolism	53:95	High-fiber diets attenuate emphysema development via modulation of gut microbiota and metabolism.					
33772084	3	1	theme	dietary	326:332	arg1	components					334:343	various dietary components	318:343	various dietary components	318:343	The gut microbiota and metabolites produced by various dietary components not only modulate immunity but also impact various organs.					
33772084	6	2	from	response	1067:1074	arg1	mice					1100:1103	CS-exposed emphysema mice	1079:1103	CS-exposed emphysema mice	1079:1103	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	9	3	from	development	1724:1734	arg1	useful					1710:1715	useful	1710:1715	useful	1710:1715	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	1	4	theme	gut	154:156	arg1	composition					166:176	the gut microbe composition	150:176	the gut microbe composition	150:176	Dietary fiber functions as a prebiotic to determine the gut microbe composition.					
33772084	4	5	theme	metabolites	593:603	arg1	role					570:573	the role	566:573	the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease	566:667	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	5	6	dep	microbiota	725:734	arg1	the					717:719	the	717:719	the	717:719	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	6	7	theme	non-fermentable	923:937	arg1	cellulose					939:947	non-fermentable cellulose	923:947	non-fermentable cellulose	923:947	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	9	8	theme	treatment	1750:1758	arg1	plan					1760:1763	a new COPD treatment plan	1739:1763	a new COPD treatment plan	1739:1763	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	4	9	theme	recent	413:418	arg1	findings					420:427	recent findings	413:427	recent findings	413:427	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	1	10	theme	microbe	158:164	arg1	composition					166:176	the gut microbe composition	150:176	the gut microbe composition	150:176	Dietary fiber functions as a prebiotic to determine the gut microbe composition.					
33772084	4	11	theme	cystic	536:541	arg1	fibrosis					543:550	cystic fibrosis	536:550	cystic fibrosis	536:550	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	7	12	theme	fiber	1160:1164	arg1	types					1143:1147	different types	1133:1147	different types of dietary fiber	1133:1164	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	7	13	theme	short-chain	1279:1289	arg1	acids					1297:1301	short-chain fatty acids	1279:1301	short-chain fatty acids	1279:1301	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	9	14	theme	systemic	1663:1670	arg1	inhibition					1685:1694	local and systemic inflammation inhibition	1653:1694	local and systemic inflammation inhibition	1653:1694	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	9	15	theme	COPD	1745:1748	arg1	plan					1760:1763	a new COPD treatment plan	1739:1763	a new COPD treatment plan	1739:1763	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	5	16	dep	-exposed	829:836	arg1	smoking					817:823	cigarette smoking	807:823	cigarette smoking	807:823	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	7	17	theme	acids	1309:1313	arg1	generation					1265:1274	the generation	1261:1274	the generation of short-chain fatty acids, bile acids, and sphingolipids	1261:1332	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	7	18	theme	bile	1304:1307	arg1	acids					1309:1313	bile acids	1304:1313	bile acids	1304:1313	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	9	19	from	useful	1710:1715	arg1	development					1724:1734	the development	1720:1734	the development of a new COPD treatment plan	1720:1763	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	6	20	theme	emphysema	1024:1032	arg1	progression					1034:1044	emphysema progression	1024:1044	emphysema progression	1024:1044	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	5	21	theme	dietary	764:770	arg1	components					778:787	dietary fiber components	764:787	dietary fiber components	764:787	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	4	22	theme	respiratory	496:506	arg1	allergy					557:563	allergy	557:563	allergy	557:563	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	4	22	theme	respiratory	496:506	arg1	diseases					508:515	several respiratory diseases	488:515	several respiratory diseases	488:515	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	4	22	theme	respiratory	496:506	arg1	asthma					528:533	asthma	528:533	asthma	528:533	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	4	22	theme	respiratory	496:506	arg1	fibrosis					543:550	cystic fibrosis	536:550	cystic fibrosis	536:550	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	9	23	theme	new	1741:1743	arg1	plan					1760:1763	a new COPD treatment plan	1739:1763	a new COPD treatment plan	1739:1763	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	8	24	theme	beneficial	1408:1417	arg1	role					1419:1422	a beneficial role	1406:1422	a beneficial role	1406:1422	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	2	25	from	functions	223:231	arg1	health					263:268	human health	257:268	human health	257:268	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	8	26	theme	high-fiber	1384:1393	arg1	diets					1395:1399	high-fiber diets	1384:1399	high-fiber diets	1384:1399	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	5	27	theme	fiber	772:776	arg1	components					778:787	dietary fiber components	764:787	dietary fiber components	764:787	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	7	28	theme	gut	1198:1200	arg1	microbiota					1202:1211	gut microbiota	1198:1211	gut microbiota	1198:1211	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	5	29	theme	-exposed	829:836	arg1	model					848:852	a cigarette smoking (CS)-exposed emphysema model	805:852	a cigarette smoking (CS)-exposed emphysema model	805:852	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	2	30	from	responses	244:252	arg1	health					263:268	human health	257:268	human health	257:268	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	7	31	theme	impacted	1232:1239	arg1	anabolism					1241:1249	differentially impacted anabolism	1217:1249	differentially impacted anabolism	1217:1249	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	7	32	theme	fatty	1291:1295	arg1	acids					1297:1301	short-chain fatty acids	1279:1301	short-chain fatty acids	1279:1301	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	7	33	theme	different	1133:1141	arg1	types					1143:1147	different types	1133:1147	different types of dietary fiber	1133:1164	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	6	34	theme	high-fiber	897:906	arg1	diet					908:911	a high-fiber diet	895:911	a high-fiber diet including non-fermentable cellulose and fermentable pectin	895:970	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	0	35	theme	High-fiber	0:9	arg1	diets					11:15	High-fiber diets	0:15	High-fiber diets	0:15	High-fiber diets attenuate emphysema development via modulation of gut microbiota and metabolism.					
33772084	9	36	theme	plan	1760:1763	arg1	development					1724:1734	the development	1720:1734	the development of a new COPD treatment plan	1720:1763	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	9	37	from	potential	1569:1577	arg1	emphysema					1639:1647	emphysema	1639:1647	emphysema	1639:1647	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	9	37	from	potential	1569:1577	arg1	diet					1631:1634	a high-fiber diet	1618:1634	a high-fiber diet	1618:1634	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	1	38	theme	Dietary	98:104	arg1	prebiotic					127:135	prebiotic	127:135	prebiotic	127:135	Dietary fiber functions as a prebiotic to determine the gut microbe composition.					
33772084	1	38	theme	Dietary	98:104	arg1	fiber					106:110	Dietary fiber	98:110	Dietary fiber	98:110	Dietary fiber functions as a prebiotic to determine the gut microbe composition.					
33772084	9	39	theme	metabolites	1601:1611	arg1	potential					1569:1577	the therapeutic potential	1553:1577	the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema	1553:1647	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	0	40	theme	emphysema	27:35	arg1	development					37:47	emphysema development	27:47	emphysema development	27:47	High-fiber diets attenuate emphysema development via modulation of gut microbiota and metabolism.					
33772084	9	41	theme	therapeutic	1557:1567	arg1	potential					1569:1577	the therapeutic potential	1553:1577	the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema	1553:1647	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	5	42	theme	gut	721:723	arg1	microbiota					725:734	gut microbiota	721:734	gut microbiota	721:734	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	8	43	theme	gut	1431:1433	arg1	modulation					1457:1466	the gut microbiota-metabolite modulation	1427:1466	the gut microbiota-metabolite modulation	1427:1466	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	4	44	theme	pulmonary	651:659	arg1	disease					661:667	pulmonary disease	651:667	pulmonary disease	651:667	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	3	45	theme	various	388:394	arg1	organs					396:401	various organs	388:401	various organs	388:401	The gut microbiota and metabolites produced by various dietary components not only modulate immunity but also impact various organs.					
33772084	4	46	theme	microbiota	578:587	arg1	role					570:573	the role	566:573	the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease	566:667	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	9	47	theme	gut	1582:1584	arg1	microbiota					1586:1595	gut microbiota	1582:1595	gut microbiota	1582:1595	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	2	48	theme	gut	183:185	arg1	microbiota					187:196	The gut microbiota	179:196	The gut microbiota	179:196	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	0	49	dep	gut	67:69	arg1	microbiota					71:80	microbiota	71:80	microbiota	71:80	High-fiber diets attenuate emphysema development via modulation of gut microbiota and metabolism.					
33772084	2	50	dep	functions	223:231	arg1	the					209:211	the	209:211	the	209:211	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	5	51	theme	cigarette	807:815	arg1	smoking					817:823	cigarette smoking	807:823	cigarette smoking	807:823	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	6	52	theme	pathological	987:998	arg1	changes					1000:1006	the pathological changes	983:1006	the pathological changes associated with emphysema progression	983:1044	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	7	53	theme	anabolism	1241:1249	arg1	diversity					1185:1193	the diversity	1181:1193	the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids	1181:1332	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	9	54	theme	inflammation	1672:1683	arg1	inhibition					1685:1694	local and systemic inflammation inhibition	1653:1694	local and systemic inflammation inhibition	1653:1694	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	7	55	theme	microbiota	1202:1211	arg1	diversity					1185:1193	the diversity	1181:1193	the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids	1181:1332	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	3	56	theme	gut	275:277	arg1	microbiota					279:288	The gut microbiota	271:288	The gut microbiota	271:288	The gut microbiota and metabolites produced by various dietary components not only modulate immunity but also impact various organs.					
33772084	4	57	theme	several	488:494	arg1	allergy					557:563	allergy	557:563	allergy	557:563	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	4	57	theme	several	488:494	arg1	diseases					508:515	several respiratory diseases	488:515	several respiratory diseases	488:515	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	4	57	theme	several	488:494	arg1	asthma					528:533	asthma	528:533	asthma	528:533	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	4	57	theme	several	488:494	arg1	fibrosis					543:550	cystic fibrosis	536:550	cystic fibrosis	536:550	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	2	58	theme	immune	237:242	arg1	responses					244:252	immune responses	237:252	immune responses	237:252	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	5	59	theme	emphysema	838:846	arg1	model					848:852	a cigarette smoking (CS)-exposed emphysema model	805:852	a cigarette smoking (CS)-exposed emphysema model	805:852	Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model.					
33772084	6	60	theme	emphysema	1090:1098	arg1	mice					1100:1103	CS-exposed emphysema mice	1079:1103	CS-exposed emphysema mice	1079:1103	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	3	61	theme	various	318:324	arg1	components					334:343	various dietary components	318:343	various dietary components	318:343	The gut microbiota and metabolites produced by various dietary components not only modulate immunity but also impact various organs.					
33772084	2	62	theme	metabolic	213:221	arg1	functions					223:231	metabolic functions	213:231	metabolic functions	213:231	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	7	63	theme	dietary	1152:1158	arg1	fiber					1160:1164	dietary fiber	1152:1164	dietary fiber	1152:1164	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	9	64	theme	local	1653:1657	arg1	inhibition					1685:1694	local and systemic inflammation inhibition	1653:1694	local and systemic inflammation inhibition	1653:1694	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	6	65	theme	fermentable	953:963	arg1	pectin					965:970	fermentable pectin	953:970	fermentable pectin	953:970	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	8	66	theme	CS-exposed	1493:1502	arg1	mice					1514:1517	CS-exposed emphysema mice	1493:1517	CS-exposed emphysema mice	1493:1517	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	8	67	theme	microbiota-metabolite	1435:1455	arg1	modulation					1457:1466	the gut microbiota-metabolite modulation	1427:1466	the gut microbiota-metabolite modulation	1427:1466	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	7	68	theme	sphingolipids	1320:1332	arg1	generation					1265:1274	the generation	1261:1274	the generation of short-chain fatty acids, bile acids, and sphingolipids	1261:1332	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	6	69	theme	CS-exposed	1079:1088	arg1	mice					1100:1103	CS-exposed emphysema mice	1079:1103	CS-exposed emphysema mice	1079:1103	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	9	70	theme	high-fiber	1620:1629	arg1	diet					1631:1634	a high-fiber diet	1618:1634	a high-fiber diet	1618:1634	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	9	71	theme	microbiota	1586:1595	arg1	potential					1569:1577	the therapeutic potential	1553:1577	the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema	1553:1647	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	4	72	theme	dietary	617:623	arg1	nutrients					625:633	dietary nutrients	617:633	dietary nutrients	617:633	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	7	73	theme	acids	1297:1301	arg1	generation					1265:1274	the generation	1261:1274	the generation of short-chain fatty acids, bile acids, and sphingolipids	1261:1332	Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids.					
33772084	4	74	theme	microbial	449:457	arg1	dysbiosis					459:467	microbial dysbiosis	449:467	microbial dysbiosis	449:467	Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear.					
33772084	2	75	theme	human	257:261	arg1	health					263:268	human health	257:268	human health	257:268	The gut microbiota influences the metabolic functions and immune responses in human health.					
33772084	9	76	from	diet	1631:1634	arg1	metabolites					1601:1611	metabolites	1601:1611	metabolites from a high-fiber diet	1601:1634	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	9	76	from	diet	1631:1634	arg1	potential					1569:1577	the therapeutic potential	1553:1577	the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema	1553:1647	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	9	76	from	diet	1631:1634	arg1	microbiota					1586:1595	gut microbiota	1582:1595	gut microbiota	1582:1595	Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.					
33772084	6	77	from	changes	1000:1006	arg1	mice					1100:1103	CS-exposed emphysema mice	1079:1103	CS-exposed emphysema mice	1079:1103	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
33772084	0	78	theme	gut	67:69	arg1	modulation					53:62	modulation	53:62	modulation of gut microbiota and metabolism	53:95	High-fiber diets attenuate emphysema development via modulation of gut microbiota and metabolism.					
33772084	8	79	theme	emphysema	1504:1512	arg1	mice					1514:1517	CS-exposed emphysema mice	1493:1517	CS-exposed emphysema mice	1493:1517	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	8	80	theme	study	1364:1368	arg1	results					1348:1354	the results	1344:1354	the results of this study	1344:1368	Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice.					
33772084	6	81	theme	inflammatory	1054:1065	arg1	response					1067:1074	the inflammatory response	1050:1074	the inflammatory response in CS-exposed emphysema mice	1050:1103	In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice.					
34956180	6	0	theme	%	942:942	arg1	DSS					944:946	5% DSS	941:946	5% DSS	941:946	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	7	1	theme	Enterobacteriaceae	1148:1165	arg1	enrichment					1167:1176	Enterobacteriaceae enrichment	1148:1176	Enterobacteriaceae enrichment	1148:1176	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	10	2	theme	acids	1794:1798	arg1	balance					1848:1854	an altered balance	1837:1854	an altered balance of T helper cell subsets	1837:1879	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	2	theme	acids	1794:1798	arg1	signaling					1822:1830	reduced NF-κB signaling	1808:1830	reduced NF-κB signaling	1808:1830	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	2	theme	acids	1794:1798	arg1	concentration					1759:1771	an increased concentration	1746:1771	an increased concentration of short chain fatty acids (SCFA)	1746:1805	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	8	3	from	production	1432:1441	arg1	colon					1457:1461	the colon	1453:1461	the colon of DPMA mice	1453:1474	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	7	4	theme	model	1137:1141	arg1	generation					1110:1119	the successful generation	1095:1119	the successful generation of a DPMA mouse model	1095:1141	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	10	5	theme	NF-κB	1816:1820	arg1	signaling					1822:1830	reduced NF-κB signaling	1808:1830	reduced NF-κB signaling	1808:1830	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	6	6	theme	antibiotic	916:925	arg1	treatment					927:935	antibiotic treatment	916:935	antibiotic treatment	916:935	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	7	7	with	consistent	1179:1188	arg1	microbiota					1222:1231	the giant panda intestinal microbiota	1195:1231	the giant panda intestinal microbiota	1195:1231	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	7	8	theme	DPMA	1126:1129	arg1	model					1137:1141	a DPMA mouse model	1124:1141	a DPMA mouse model	1124:1141	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	2	9	theme	intestinal	293:302	arg1	damage					315:320	intestinal epithelium damage	293:320	intestinal epithelium damage	293:320	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	1	10	theme	giant	194:198	arg1	death					206:210	captive giant panda death	186:210	captive giant panda death	186:210	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	10	11	from	changes	1691:1697	arg1	composition					1713:1723	microbiota composition	1702:1723	microbiota composition	1702:1723	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	7	12	theme	panda	1205:1209	arg1	microbiota					1222:1231	the giant panda intestinal microbiota	1195:1231	the giant panda intestinal microbiota	1195:1231	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	8	13	theme	intestinal	1299:1308	arg1	inflammation					1310:1321	intestinal inflammation	1299:1321	intestinal inflammation	1299:1321	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	1	14	theme	death	206:210	arg1	causes					176:181	the main causes	167:181	the main causes of captive giant panda death	167:210	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	7	15	theme	successful	1099:1108	arg1	generation					1110:1119	the successful generation	1095:1119	the successful generation of a DPMA mouse model	1095:1141	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	5	16	from	inflammation	778:789	arg1	model					877:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	2	17	theme	secondary	326:334	arg1	infection					347:355	secondary intestinal infection	326:355	secondary intestinal infection	326:355	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	9	18	theme	G83	1512:1514	arg1	administration					1481:1494	The administration	1477:1494	The administration of L. plantarum G83	1477:1514	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	10	19	theme	microbiota	1702:1711	arg1	composition					1713:1723	microbiota composition	1702:1723	microbiota composition	1702:1723	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	8	20	theme	intestinal	1333:1342	arg1	expression					1367:1376	intestinal tight junction protein expression	1333:1376	intestinal tight junction protein expression (ZO-1, Occludin)	1333:1393	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	2	21	theme	gastrointestinal	246:261	arg1	tract					263:267	gastrointestinal tract	246:267	gastrointestinal tract structure	246:277	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	0	22	from	Response	78:85	arg1	Mice					130:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	8	23	theme	junction	1350:1357	arg1	expression					1367:1376	intestinal tight junction protein expression	1333:1376	intestinal tight junction protein expression (ZO-1, Occludin)	1333:1393	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	3	24	theme	giant	370:374	arg1	panda					376:380	The captive giant panda	358:380	The captive giant panda	358:380	The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.					
34956180	10	25	theme	cell	1868:1871	arg1	subsets					1873:1879	T helper cell subsets	1859:1879	T helper cell subsets	1859:1879	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	9	26	theme	associated	1574:1583	arg1	coli					1601:1604	E. coli	1598:1604	E. coli	1598:1604	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	9	26	theme	associated	1574:1583	arg1	taxa					1585:1588	pathogen associated taxa	1565:1588	pathogen associated taxa such as E. coli	1565:1604	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	6	27	theme	intestinal	1022:1031	arg1	inflammation					1033:1044	intestinal inflammation	1022:1044	intestinal inflammation	1022:1044	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	0	28	theme	DSS-Induced	90:100	arg1	Mice					130:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	6	29	theme	G83	1015:1017	arg1	effect					992:997	the effect	988:997	the effect of L. plantarum G83 on intestinal inflammation and microbiota	988:1059	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	4	30	theme	panda	550:554	arg1	enteritis					556:564	giant panda enteritis	544:564	giant panda enteritis	544:564	However, there are few reported probiotics to treat giant panda enteritis and the associated dysbiosis.					
34956180	9	31	theme	beneficial	1634:1643	arg1	bacteria					1645:1652	beneficial bacteria	1634:1652	beneficial bacteria including Bifidobacterium spp	1634:1682	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	9	31	theme	beneficial	1634:1643	arg1	spp					1680:1682	Bifidobacterium spp	1664:1682	Bifidobacterium spp	1664:1682	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	0	32	theme	Microbiota-Associated	108:128	arg1	Mice					130:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	5	33	theme	Lactobacillus	648:660	arg1	probiotic					726:734	probiotic	726:734	probiotic	726:734	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	5	33	theme	Lactobacillus	648:660	arg1	G201683					672:678	Lactobacillus plantarum G201683	648:678	Lactobacillus plantarum G201683 (L. plantarum G83)	648:697	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	4	34	theme	few	511:513	arg1	probiotics					524:533	few reported probiotics	511:533	few reported probiotics to treat giant panda enteritis and the associated dysbiosis	511:593	However, there are few reported probiotics to treat giant panda enteritis and the associated dysbiosis.					
34956180	5	35	theme	mouse	871:875	arg1	model					877:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	11	36	theme	giant	1985:1989	arg1	panda					1991:1995	the giant panda	1981:1995	the giant panda	1981:1995	Our findings support L. plantarum G83 as a promising probiotic to treat intestinal inflammation in the giant panda.					
34956180	8	37	theme	inflammatory	1410:1421	arg1	production					1432:1441	inflammatory cytokine production	1410:1441	inflammatory cytokine production (TNF-) in the colon of DPMA mice	1410:1474	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	8	37	theme	inflammatory	1410:1421	arg1	TNF-					1444:1447	TNF-	1444:1447	TNF-	1444:1447	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	3	38	theme	artificial	450:459	arg1	feeding					461:467	long-term artificial feeding	440:467	long-term artificial feeding	440:467	The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.					
34956180	5	39	theme	microbiota-associated	842:862	arg1	model					877:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	7	40	theme	giant	1199:1203	arg1	microbiota					1222:1231	the giant panda intestinal microbiota	1195:1231	the giant panda intestinal microbiota	1195:1231	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	10	41	theme	reduced	1808:1814	arg1	signaling					1822:1830	reduced NF-κB signaling	1808:1830	reduced NF-κB signaling	1808:1830	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	1	42	theme	Intestinal	136:145	arg1	diseases					147:154	Intestinal diseases	136:154	Intestinal diseases	136:154	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	5	43	dep	L.	681:682	arg1	plantarum					684:692	L. plantarum	681:692	L. plantarum G83	681:696	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	7	44	theme	intestinal	1211:1220	arg1	microbiota					1222:1231	the giant panda intestinal microbiota	1195:1231	the giant panda intestinal microbiota	1195:1231	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	0	45	theme	Lactobacillus	18:30	arg1	G201683					42:48	The Panda-Derived Lactobacillus plantarum G201683	0:48	The Panda-Derived Lactobacillus plantarum G201683	0:48	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	6	46	theme	DPMA	888:891	arg1	mouse					893:897	The DPMA mouse	884:897	The DPMA mouse	884:897	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	5	47	dep	G201683	672:678	arg1	L.					681:682	L.	681:682	L.	681:682	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	5	47	dep	G201683	672:678	arg1	G83					694:696	G83	694:696	G83	694:696	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	10	48	theme	fatty	1788:1792	arg1	SCFA					1801:1804	SCFA	1801:1804	SCFA	1801:1804	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	48	theme	fatty	1788:1792	arg1	acids					1794:1798	short chain fatty acids	1776:1798	short chain fatty acids (SCFA)	1776:1805	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	5	49	theme	induced	828:834	arg1	model					877:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	4	50	theme	associated	574:583	arg1	dysbiosis					585:593	the associated dysbiosis	570:593	the associated dysbiosis	570:593	However, there are few reported probiotics to treat giant panda enteritis and the associated dysbiosis.					
34956180	2	51	theme	special	219:225	arg1	habits					235:240	Their special dietary habits	213:240	Their special dietary habits	213:240	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	8	52	theme	DPMA	1466:1469	arg1	mice					1471:1474	DPMA mice	1466:1474	DPMA mice	1466:1474	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	10	53	theme	short	1776:1780	arg1	SCFA					1801:1804	SCFA	1801:1804	SCFA	1801:1804	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	53	theme	short	1776:1780	arg1	acids					1794:1798	short chain fatty acids	1776:1798	short chain fatty acids (SCFA)	1776:1805	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	5	54	theme	DPMA	865:868	arg1	model					877:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	1	55	theme	main	171:174	arg1	causes					176:181	the main causes	167:181	the main causes of captive giant panda death	167:210	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	6	56	theme	5	941:941	arg1	%					942:942	%	942:942	%	942:942	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	7	57	theme	mouse	1131:1135	arg1	model					1137:1141	a DPMA mouse model	1124:1141	a DPMA mouse model	1124:1141	Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota.					
34956180	2	58	theme	epithelium	304:313	arg1	damage					315:320	intestinal epithelium damage	293:320	intestinal epithelium damage	293:320	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	1	59	theme	captive	186:192	arg1	death					206:210	captive giant panda death	186:210	captive giant panda death	186:210	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	8	60	theme	histological	1274:1285	arg1	severity					1287:1294	clinical and histological severity	1261:1294	clinical and histological severity of intestinal inflammation	1261:1321	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	10	61	theme	altered	1840:1846	arg1	balance					1848:1854	an altered balance	1837:1854	an altered balance of T helper cell subsets	1837:1879	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	1	62	theme	panda	200:204	arg1	death					206:210	captive giant panda death	186:210	captive giant panda death	186:210	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	2	63	theme	intestinal	336:345	arg1	infection					347:355	secondary intestinal infection	326:355	secondary intestinal infection	326:355	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	8	64	theme	inflammation	1310:1321	arg1	severity					1287:1294	clinical and histological severity	1261:1294	clinical and histological severity of intestinal inflammation	1261:1321	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	5	65	theme	protective	746:755	arg1	effect					757:762	a protective effect	744:762	a protective effect	744:762	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	2	66	theme	tract	263:267	arg1	structure					269:277	gastrointestinal tract structure	246:277	gastrointestinal tract structure	246:277	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	11	67	from	inflammation	1965:1976	arg1	panda					1991:1995	the giant panda	1981:1995	the giant panda	1981:1995	Our findings support L. plantarum G83 as a promising probiotic to treat intestinal inflammation in the giant panda.					
34956180	9	68	theme	microbiota	1528:1537	arg1	composition					1539:1549	the microbiota composition	1524:1549	the microbiota composition	1524:1549	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	8	69	theme	clinical	1261:1268	arg1	severity					1287:1294	clinical and histological severity	1261:1294	clinical and histological severity of intestinal inflammation	1261:1321	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	8	70	theme	tight	1344:1348	arg1	expression					1367:1376	intestinal tight junction protein expression	1333:1376	intestinal tight junction protein expression (ZO-1, Occludin)	1333:1393	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	8	71	theme	protein	1359:1365	arg1	expression					1367:1376	intestinal tight junction protein expression	1333:1376	intestinal tight junction protein expression (ZO-1, Occludin)	1333:1393	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	10	72	theme	subsets	1873:1879	arg1	balance					1848:1854	an altered balance	1837:1854	an altered balance of T helper cell subsets	1837:1879	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	72	theme	subsets	1873:1879	arg1	signaling					1822:1830	reduced NF-κB signaling	1808:1830	reduced NF-κB signaling	1808:1830	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	72	theme	subsets	1873:1879	arg1	concentration					1759:1771	an increased concentration	1746:1771	an increased concentration of short chain fatty acids (SCFA)	1746:1805	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	9	73	theme	pathogen	1565:1572	arg1	coli					1601:1604	E. coli	1598:1604	E. coli	1598:1604	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	9	73	theme	pathogen	1565:1572	arg1	taxa					1585:1588	pathogen associated taxa	1565:1588	pathogen associated taxa such as E. coli	1565:1604	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	4	74	theme	giant	544:548	arg1	enteritis					556:564	giant panda enteritis	544:564	giant panda enteritis	544:564	However, there are few reported probiotics to treat giant panda enteritis and the associated dysbiosis.					
34956180	5	75	theme	intestinal	767:776	arg1	inflammation					778:789	intestinal inflammation	767:789	intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	767:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	0	76	theme	Panda	102:106	arg1	Mice					130:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	DSS-Induced Panda Microbiota-Associated Mice	90:133	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	1	77	theme	causes	176:181	arg1	one					160:162	one	160:162	one	160:162	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	1	77	theme	causes	176:181	arg1	causes					176:181	the main causes	167:181	the main causes of captive giant panda death	167:210	Intestinal diseases are one of the main causes of captive giant panda death.					
34956180	3	78	theme	captive	362:368	arg1	panda					376:380	The captive giant panda	358:380	The captive giant panda	358:380	The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.					
34956180	4	79	theme	reported	515:522	arg1	probiotics					524:533	few reported probiotics	511:533	few reported probiotics to treat giant panda enteritis and the associated dysbiosis	511:593	However, there are few reported probiotics to treat giant panda enteritis and the associated dysbiosis.					
34956180	5	80	theme	plantarum	662:670	arg1	probiotic					726:734	probiotic	726:734	probiotic	726:734	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	5	80	theme	plantarum	662:670	arg1	G201683					672:678	Lactobacillus plantarum G201683	648:678	Lactobacillus plantarum G201683 (L. plantarum G83)	648:697	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	11	81	dep	L.	1903:1904	arg1	plantarum					1906:1914	L. plantarum G83	1903:1918	L. plantarum G83	1903:1918	Our findings support L. plantarum G83 as a promising probiotic to treat intestinal inflammation in the giant panda.					
34956180	11	81	dep	L.	1903:1904	arg1	G83					1916:1918	G83	1916:1918	G83	1916:1918	Our findings support L. plantarum G83 as a promising probiotic to treat intestinal inflammation in the giant panda.					
34956180	11	82	theme	intestinal	1954:1963	arg1	inflammation					1965:1976	intestinal inflammation	1954:1976	intestinal inflammation in the giant panda	1954:1995	Our findings support L. plantarum G83 as a promising probiotic to treat intestinal inflammation in the giant panda.					
34956180	5	83	theme	panda	836:840	arg1	model					877:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model	794:881	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	8	84	theme	cytokine	1423:1430	arg1	production					1432:1441	inflammatory cytokine production	1410:1441	inflammatory cytokine production (TNF-) in the colon of DPMA mice	1410:1474	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	8	84	theme	cytokine	1423:1430	arg1	TNF-					1444:1447	TNF-	1444:1447	TNF-	1444:1447	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	3	85	theme	antibiotic	473:482	arg1	misuse					484:489	antibiotic misuse	473:489	antibiotic misuse	473:489	The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.					
34956180	0	86	theme	plantarum	32:40	arg1	G201683					42:48	The Panda-Derived Lactobacillus plantarum G201683	0:48	The Panda-Derived Lactobacillus plantarum G201683	0:48	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	10	87	theme	helper	1861:1866	arg1	subsets					1873:1879	T helper cell subsets	1859:1879	T helper cell subsets	1859:1879	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	3	88	theme	microbiota	412:421	arg1	dysbiosis					423:431	microbiota dysbiosis	412:431	microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse	412:489	The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.					
34956180	10	89	theme	increased	1749:1757	arg1	concentration					1759:1771	an increased concentration	1746:1771	an increased concentration of short chain fatty acids (SCFA)	1746:1805	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	9	90	theme	bacteria	1645:1652	arg1	abundance					1621:1629	abundance	1621:1629	abundance of beneficial bacteria including Bifidobacterium spp	1621:1682	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	6	91	from	effect	992:997	arg1	inflammation					1033:1044	intestinal inflammation	1022:1044	intestinal inflammation	1022:1044	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	6	91	from	effect	992:997	arg1	microbiota					1050:1059	microbiota	1050:1059	microbiota	1050:1059	The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo.					
34956180	0	92	theme	Inflammatory	65:76	arg1	Response					78:85	the Inflammatory Response	61:85	the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice	61:133	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
34956180	9	93	theme	Bifidobacterium	1664:1678	arg1	spp					1680:1682	Bifidobacterium spp	1664:1682	Bifidobacterium spp	1664:1682	The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp.					
34956180	3	94	theme	long-term	440:448	arg1	feeding					461:467	long-term artificial feeding	440:467	long-term artificial feeding	440:467	The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse.					
34956180	8	95	dep	L.	1234:1235	arg1	plantarum					1237:1245	L. plantarum G83	1234:1249	L. plantarum G83	1234:1249	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	8	95	dep	L.	1234:1235	arg1	G83					1247:1249	G83	1247:1249	G83	1247:1249	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	2	96	theme	dietary	227:233	arg1	habits					235:240	Their special dietary habits	213:240	Their special dietary habits	213:240	Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection.					
34956180	10	97	theme	chain	1782:1786	arg1	SCFA					1801:1804	SCFA	1801:1804	SCFA	1801:1804	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	10	97	theme	chain	1782:1786	arg1	acids					1794:1798	short chain fatty acids	1776:1798	short chain fatty acids (SCFA)	1776:1805	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	8	98	dep	expression	1367:1376	arg1	ZO-1					1379:1382	ZO-1	1379:1382	ZO-1	1379:1382	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	8	98	dep	expression	1367:1376	arg1	Occludin					1385:1392	Occludin	1385:1392	Occludin	1385:1392	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	10	99	theme	T	1859:1859	arg1	subsets					1873:1879	T helper cell subsets	1859:1879	T helper cell subsets	1859:1879	These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets.					
34956180	5	100	link	panda-derived	712:724	arg1	probiotic					726:734	probiotic	726:734	probiotic	726:734	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	5	100	link	panda-derived	712:724	arg1	G201683					672:678	Lactobacillus plantarum G201683	648:678	Lactobacillus plantarum G201683 (L. plantarum G83)	648:697	This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model.					
34956180	8	101	theme	mice	1471:1474	arg1	colon					1457:1461	the colon	1453:1461	the colon of DPMA mice	1453:1474	L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice.					
34956180	0	102	theme	Panda-Derived	4:16	arg1	G201683					42:48	The Panda-Derived Lactobacillus plantarum G201683	0:48	The Panda-Derived Lactobacillus plantarum G201683	0:48	The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.					
31963640	8	0	theme	plasma	1148:1153	arg1	OCFAs					1155:1159	plasma OCFAs	1148:1159	plasma OCFAs	1148:1159	Moreover, the dietary SF increased circulating levels of plasma OCFAs, especially C17:0.					
31963640	8	0	theme	plasma	1148:1153	arg1	C17:0					1173:1177	C17:0	1173:1177	C17:0	1173:1177	Moreover, the dietary SF increased circulating levels of plasma OCFAs, especially C17:0.					
31963640	1	1	theme	insulin	268:274	arg1	sensitivity					276:286	insulin sensitivity	268:286	insulin sensitivity	268:286	The transition from pregnancy to lactation is characterized by a progressive decrease in insulin sensitivity.					
31963640	3	2	theme	heptadecanoic	488:500	arg1	acid					502:505	heptadecanoic acid	488:505	heptadecanoic acid (C17:0)	488:513	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	3	2	theme	heptadecanoic	488:500	arg1	C17:0					508:512	C17:0	508:512	C17:0	508:512	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	8	3	theme	dietary	1105:1111	arg1	SF					1113:1114	the dietary SF	1101:1114	the dietary SF	1101:1114	Moreover, the dietary SF increased circulating levels of plasma OCFAs, especially C17:0.					
31963640	0	4	theme	Gut	61:63	arg1	Microbiota					65:74	the Gut Microbiota	57:74	the Gut Microbiota	57:74	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	0	5	theme	Insulin	150:156	arg1	Sensitivity					158:168	Insulin Sensitivity	150:168	Insulin Sensitivity in Sows	150:176	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	10	6	theme	plasma	1389:1394	arg1	glucose					1396:1402	plasma glucose	1389:1402	plasma glucose	1389:1402	Furthermore, the plasma C15:0 and C17:0 was negatively correlated with the area under curve of plasma glucose after meal and plasma interleukin-6.					
31963640	5	7	theme	dietary	885:891	arg1	treatment					893:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	6	8	theme	microbiota	982:991	arg1	structure					949:957	structure	949:957	structure	949:957	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	6	8	theme	microbiota	982:991	arg1	composition					963:973	composition	963:973	composition	963:973	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	0	9	from	Inclusion	0:8	arg1	Diet					44:47	the Gestation Diet	30:47	the Gestation Diet	30:47	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	9	10	contain	had	1196:1198	arg2	sensitivity					1217:1227	a higher insulin sensitivity	1200:1227	a higher insulin sensitivity	1200:1227	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	9	10	contain	had	1196:1198	arg2	level					1263:1267	a lower systemic inflammation level	1233:1267	a lower systemic inflammation level	1233:1267	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	9	10	contain	had	1196:1198	arg1	sows					1191:1194	The SF-fed sows	1180:1194	The SF-fed sows	1180:1194	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	11	11	theme	perinatal	1536:1544	arg1	sows					1546:1549	perinatal sows	1536:1549	perinatal sows	1536:1549	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	0	12	dep	Propionate	92:101	arg1	Levels					129:134	Levels	129:134	Levels	129:134	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	11	13	theme	systemic	1511:1518	arg1	inflammation					1520:1531	systemic inflammation	1511:1531	systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production	1511:1631	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	6	14	theme	gut	978:980	arg1	microbiota					982:991	gut microbiota	978:991	gut microbiota	978:991	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	8	15	theme	OCFAs	1155:1159	arg1	levels					1138:1143	circulating levels	1126:1143	circulating levels of plasma OCFAs, especially C17:0	1126:1177	Moreover, the dietary SF increased circulating levels of plasma OCFAs, especially C17:0.					
31963640	10	16	theme	plasma	1419:1424	arg1	interleukin-6					1426:1438	plasma interleukin-6	1419:1438	plasma interleukin-6	1419:1438	Furthermore, the plasma C15:0 and C17:0 was negatively correlated with the area under curve of plasma glucose after meal and plasma interleukin-6.					
31963640	9	17	theme	SF-fed	1184:1189	arg1	sows					1191:1194	The SF-fed sows	1180:1194	The SF-fed sows	1180:1194	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	4	18	theme	gut	704:706	arg1	microbiota					708:717	gut microbiota	704:717	gut microbiota	704:717	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	1	19	theme	progressive	244:254	arg1	decrease					256:263	a progressive decrease	242:263	a progressive decrease in insulin sensitivity	242:286	The transition from pregnancy to lactation is characterized by a progressive decrease in insulin sensitivity.					
31963640	5	20	theme	guar	833:836	arg1	gum					838:840	2.0% guar gum	828:840	2.0% guar gum	828:840	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	4	21	from	sensitivity	766:776	arg1	sows					781:784	sows	781:784	sows	781:784	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	11	22	from	inflammation	1520:1531	arg1	sows					1546:1549	perinatal sows	1536:1549	perinatal sows	1536:1549	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	2	23	theme	insulin	371:377	arg1	sensitivity					379:389	insulin sensitivity	371:389	insulin sensitivity	371:389	Propionate increases with dietary fiber consumption and has been shown to improve insulin sensitivity.					
31963640	3	24	theme	fatty	437:441	arg1	acids					443:447	plasma odd-chain fatty acids	420:447	plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance	420:564	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	0	25	theme	Plasma	85:90	arg1	Propionate					92:101	Plasma Propionate	85:101	Plasma Propionate	85:101	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	5	26	theme	waxy	862:865	arg1	treatment					893:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	3	27	link	gut-derived	600:610	arg1	propionate					612:621	gut-derived propionate	600:621	gut-derived propionate	600:621	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	1	28	from	decrease	256:263	arg1	sensitivity					276:286	insulin sensitivity	268:286	insulin sensitivity	268:286	The transition from pregnancy to lactation is characterized by a progressive decrease in insulin sensitivity.					
31963640	11	29	theme	stimulating	1579:1589	arg1	effect					1591:1596	its stimulating effect	1575:1596	its stimulating effect on propionate and OCFAs production	1575:1631	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	3	30	theme	insulin	547:553	arg1	resistance					555:564	insulin resistance	547:564	insulin resistance	547:564	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	4	31	from	effects	659:665	arg1	microbiota					708:717	gut microbiota	704:717	gut microbiota	704:717	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	4	31	from	effects	659:665	arg1	acids					748:752	plasma non-esterified fatty acids	720:752	plasma non-esterified fatty acids	720:752	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	4	31	from	effects	659:665	arg1	sensitivity					766:776	insulin sensitivity	758:776	insulin sensitivity	758:776	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	3	32	theme	plasma	420:425	arg1	acids					443:447	plasma odd-chain fatty acids	420:447	plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance	420:564	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	9	33	theme	systemic	1241:1248	arg1	level					1263:1267	a lower systemic inflammation level	1233:1267	a lower systemic inflammation level	1233:1267	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	4	34	theme	plasma	720:725	arg1	acids					748:752	plasma non-esterified fatty acids	720:752	plasma non-esterified fatty acids	720:752	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	4	35	from	acids	748:752	arg1	sows					781:784	sows	781:784	sows	781:784	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	4	36	theme	fatty	742:746	arg1	acids					748:752	plasma non-esterified fatty acids	720:752	plasma non-esterified fatty acids	720:752	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	7	37	theme	intestinal	1057:1066	arg1	production					1079:1088	intestinal propionate production	1057:1088	intestinal propionate production	1057:1088	Genus Eubacterium increased by SF addition may promote intestinal propionate production.					
31963640	3	38	theme	odd-chain	427:435	arg1	acids					443:447	plasma odd-chain fatty acids	420:447	plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance	420:564	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	0	39	theme	Fiber	21:25	arg1	Inclusion					0:8	Inclusion	0:8	Inclusion of Soluble Fiber in the Gestation Diet	0:47	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	9	40	theme	higher	1202:1207	arg1	sensitivity					1217:1227	a higher insulin sensitivity	1200:1227	a higher insulin sensitivity	1200:1227	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	0	41	theme	Odd-Chain	107:115	arg1	Acids					123:127	Odd-Chain Fatty Acids	107:127	Odd-Chain Fatty Acids	107:127	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	9	42	theme	insulin	1209:1215	arg1	sensitivity					1217:1227	a higher insulin sensitivity	1200:1227	a higher insulin sensitivity	1200:1227	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	4	43	theme	insulin	758:764	arg1	sensitivity					766:776	insulin sensitivity	758:776	insulin sensitivity	758:776	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	10	44	theme	plasma	1311:1316	arg1	C15:0					1318:1322	the plasma C15:0	1307:1322	the plasma C15:0	1307:1322	Furthermore, the plasma C15:0 and C17:0 was negatively correlated with the area under curve of plasma glucose after meal and plasma interleukin-6.					
31963640	3	45	theme	pentadecanoic	457:469	arg1	C15:0					477:481	C15:0	477:481	C15:0	477:481	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	3	45	theme	pentadecanoic	457:469	arg1	acid					471:474	pentadecanoic acid	457:474	pentadecanoic acid (C15:0)	457:482	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	0	46	theme	Soluble	13:19	arg1	Fiber					21:25	Soluble Fiber	13:25	Soluble Fiber	13:25	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	0	47	from	Sensitivity	158:168	arg1	Sows					173:176	Sows	173:176	Sows	173:176	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	7	48	theme	propionate	1068:1077	arg1	production					1079:1088	intestinal propionate production	1057:1088	intestinal propionate production	1057:1088	Genus Eubacterium increased by SF addition may promote intestinal propionate production.					
31963640	4	49	theme	present	628:634	arg1	study					636:640	The present study	624:640	The present study	624:640	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	4	50	theme	fiber	678:682	arg1	effects					659:665	the effects	655:665	the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows	655:784	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	6	51	from	composition	963:973	arg1	sows					996:999	sows	996:999	sows	996:999	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	4	52	theme	soluble	670:676	arg1	fiber					678:682	soluble fiber	670:682	soluble fiber during gestation	670:699	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	5	53	theme	pregelatinized	847:860	arg1	treatment					893:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	0	54	theme	Fatty	117:121	arg1	Acids					123:127	Odd-Chain Fatty Acids	107:127	Odd-Chain Fatty Acids	107:127	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	7	55	theme	Genus	1002:1006	arg1	Eubacterium					1008:1018	Genus Eubacterium	1002:1018	Genus Eubacterium increased by SF addition	1002:1043	Genus Eubacterium increased by SF addition may promote intestinal propionate production.					
31963640	3	56	theme	Recent	392:397	arg1	studies					399:405	Recent studies	392:405	Recent studies	392:405	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	5	57	theme	maize	867:871	arg1	treatment					893:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	7	58	theme	SF	1033:1034	arg1	addition					1036:1043	SF addition	1033:1043	SF addition	1033:1043	Genus Eubacterium increased by SF addition may promote intestinal propionate production.					
31963640	0	59	theme	Gestation	34:42	arg1	Diet					44:47	the Gestation Diet	30:47	the Gestation Diet	30:47	Inclusion of Soluble Fiber in the Gestation Diet Changes the Gut Microbiota, Affects Plasma Propionate and Odd-Chain Fatty Acids Levels, and Improves Insulin Sensitivity in Sows.					
31963640	6	60	theme	SF	925:926	arg1	addition					928:935	The SF addition	921:935	The SF addition	921:935	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	6	61	from	structure	949:957	arg1	sows					996:999	sows	996:999	sows	996:999	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	6	62	dep	structure	949:957	arg1	the					945:947	the	945:947	the	945:947	The SF addition changes the structure and composition of gut microbiota in sows.					
31963640	3	63	dep	acids	443:447	arg1	[OCFAs					449:454	[OCFAs	449:454	[OCFAs	449:454	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	11	64	theme	insulin	1476:1482	arg1	sensitivity					1484:1494	insulin sensitivity	1476:1494	insulin sensitivity	1476:1494	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	10	65	theme	glucose	1396:1402	arg1	curve					1380:1384	curve	1380:1384	curve of plasma glucose after meal and plasma interleukin-6	1380:1438	Furthermore, the plasma C15:0 and C17:0 was negatively correlated with the area under curve of plasma glucose after meal and plasma interleukin-6.					
31963640	1	66	from	pregnancy	199:207	arg1	transition					183:192	The transition	179:192	The transition from pregnancy to lactation	179:220	The transition from pregnancy to lactation is characterized by a progressive decrease in insulin sensitivity.					
31963640	9	67	theme	inflammation	1250:1261	arg1	level					1263:1267	a lower systemic inflammation level	1233:1267	a lower systemic inflammation level	1233:1267	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	3	68	dep	[OCFAs	449:454	arg1	acid					502:505	heptadecanoic acid	488:505	heptadecanoic acid (C17:0)	488:513	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	3	68	dep	[OCFAs	449:454	arg1	C17:0					508:512	C17:0	508:512	C17:0	508:512	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	3	68	dep	[OCFAs	449:454	arg1	C15:0					477:481	C15:0	477:481	C15:0	477:481	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	3	68	dep	[OCFAs	449:454	arg1	acid					471:474	pentadecanoic acid	457:474	pentadecanoic acid (C15:0)	457:482	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	11	69	from	effect	1591:1596	arg1	propionate					1601:1610	propionate	1601:1610	propionate	1601:1610	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	11	69	from	effect	1591:1596	arg1	production					1622:1631	OCFAs production	1616:1631	OCFAs production	1616:1631	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	2	70	theme	fiber	323:327	arg1	consumption					329:339	dietary fiber consumption	315:339	dietary fiber consumption	315:339	Propionate increases with dietary fiber consumption and has been shown to improve insulin sensitivity.					
31963640	5	71	theme	starch	873:878	arg1	treatment					893:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	2	72	theme	dietary	315:321	arg1	consumption					329:339	dietary fiber consumption	315:339	dietary fiber consumption	315:339	Propionate increases with dietary fiber consumption and has been shown to improve insulin sensitivity.					
31963640	4	73	theme	non-esterified	727:740	arg1	acids					748:752	plasma non-esterified fatty acids	720:752	plasma non-esterified fatty acids	720:752	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	11	74	theme	related	1564:1570	arg1	sows					1546:1549	perinatal sows	1536:1549	perinatal sows	1536:1549	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	3	75	theme	gut-derived	600:610	arg1	propionate					612:621	gut-derived propionate	600:621	gut-derived propionate	600:621	Recent studies suggest that plasma odd-chain fatty acids [OCFAs; pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0)] that inversely correlated with insulin resistance are synthesized endogenously from gut-derived propionate.					
31963640	5	76	theme	%	831:831	arg1	gum					838:840	2.0% guar gum	828:840	2.0% guar gum	828:840	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	11	77	theme	dietary	1456:1462	arg1	SF					1464:1465	dietary SF	1456:1465	dietary SF	1456:1465	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	4	78	from	microbiota	708:717	arg1	sows					781:784	sows	781:784	sows	781:784	The present study investigated the effects of soluble fiber during gestation on gut microbiota, plasma non-esterified fatty acids and insulin sensitivity in sows.					
31963640	8	79	theme	circulating	1126:1136	arg1	levels					1138:1143	circulating levels	1126:1143	circulating levels of plasma OCFAs, especially C17:0	1126:1177	Moreover, the dietary SF increased circulating levels of plasma OCFAs, especially C17:0.					
31963640	9	80	theme	perinatal	1276:1284	arg1	period					1286:1291	perinatal period	1276:1291	perinatal period	1276:1291	The SF-fed sows had a higher insulin sensitivity and a lower systemic inflammation level during perinatal period.					
31963640	5	81	theme	2.0	828:830	arg1	%					831:831	%	831:831	%	831:831	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
31963640	11	82	theme	OCFAs	1616:1620	arg1	production					1622:1631	OCFAs production	1616:1631	OCFAs production	1616:1631	In conclusion, dietary SF improves insulin sensitivity and alleviates systemic inflammation in perinatal sows, potentially related to its stimulating effect on propionate and OCFAs production.					
31963640	5	83	theme	SF	881:882	arg1	treatment					893:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	pregelatinized waxy maize starch (SF) dietary treatment	847:901	Sows were allocated to either control or 2.0% guar gum plus pregelatinized waxy maize starch (SF) dietary treatment during gestation.					
32502609	8	0	dep	50.35	1210:1214	arg1	up					1204:1205	up	1204:1205	up	1204:1205	The textile effluent colour was removed up to 50.35 and 54.95% using raw and modified clay, respectively.					
32502609	2	1	theme	electron	316:323	arg1	SEM					337:339	SEM	337:339	SEM	337:339	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	2	1	theme	electron	316:323	arg1	microscope					325:334	scanning electron microscope	307:334	scanning electron microscope (SEM)	307:340	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	1	2	theme	Rose	233:236	arg1	elimination					198:208	the elimination	194:208	the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes	194:252	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	0	3	theme	Kinetic	78:84	arg1	studies					117:123	Kinetic, equilibrium and thermodynamic studies	78:123	Kinetic, equilibrium and thermodynamic studies	78:123	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	6	4	theme	free	833:836	arg1	ΔG°					846:848	ΔG°	846:848	ΔG°	846:848	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	6	4	theme	free	833:836	arg1	energy					838:843	Gibbs free energy	827:843	Gibbs free energy (ΔG°)	827:849	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	6	5	from	exothermic	907:916	arg1	nature					947:952	nature	947:952	nature	947:952	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	7	6	from	temperature	1109:1119	arg1	stable					1092:1097	stable	1092:1097	stable	1092:1097	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	2	7	theme	scanning	307:314	arg1	SEM					337:339	SEM	337:339	SEM	337:339	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	2	7	theme	scanning	307:314	arg1	microscope					325:334	scanning electron microscope	307:334	scanning electron microscope (SEM)	307:340	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	9	8	theme	RFRN	1353:1356	arg1	option					1410:1415	a viable option	1401:1415	a viable option for the treatment of industrial wastewater and textile effluents	1401:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	8	theme	RFRN	1353:1356	arg1	BFBN					1344:1347	synthetic BFBN	1334:1347	synthetic BFBN	1334:1347	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	8	theme	RFRN	1353:1356	arg1	dyes					1358:1361	RFRN dyes	1353:1361	RFRN dyes	1353:1361	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	1	9	theme	FRN	238:240	arg1	dyes					249:252	FRN (RFRN) dyes	238:252	FRN (RFRN) dyes	238:252	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	6	10	theme	Gibbs	827:831	arg1	ΔG°					846:848	ΔG°	846:848	ΔG°	846:848	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	6	10	theme	Gibbs	827:831	arg1	energy					838:843	Gibbs free energy	827:843	Gibbs free energy (ΔG°)	827:849	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	2	11	with	interaction	426:436	arg1	adsorbents					459:468	adsorbents	459:468	adsorbents	459:468	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	0	12	dep	studies	117:123	arg1	sodium-alginate					16:30	sodium-alginate	16:30	sodium-alginate	16:30	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	0	12	dep	studies	117:123	arg1	Biocomposite					0:11	Biocomposite	0:11	Biocomposite of sodium-alginate with acidified clay for wastewater treatment	0:75	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	0	13	theme	equilibrium	87:97	arg1	studies					117:123	Kinetic, equilibrium and thermodynamic studies	78:123	Kinetic, equilibrium and thermodynamic studies	78:123	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	9	14	theme	aqueous	1368:1374	arg1	solution					1376:1383	aqueous solution	1368:1383	aqueous solution	1368:1383	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	15	from	solution	1376:1383	arg1	option					1410:1415	a viable option	1401:1415	a viable option for the treatment of industrial wastewater and textile effluents	1401:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	15	from	solution	1376:1383	arg1	adsorption					1320:1329	adsorption	1320:1329	adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents	1320:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	15	from	solution	1376:1383	arg1	BFBN					1344:1347	synthetic BFBN	1334:1347	synthetic BFBN	1334:1347	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	15	from	solution	1376:1383	arg1	dyes					1358:1361	RFRN dyes	1353:1361	RFRN dyes	1353:1361	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	3	16	theme	successful	493:502	arg1	interaction					504:514	a successful interaction	491:514	a successful interaction between adsorbent and dyes ions	491:546	The analysis showed a successful interaction between adsorbent and dyes ions.					
32502609	1	17	theme	RFRN	243:246	arg1	dyes					249:252	FRN (RFRN) dyes	238:252	FRN (RFRN) dyes	238:252	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	8	18	theme	textile	1168:1174	arg1	colour					1185:1190	The textile effluent colour	1164:1190	The textile effluent colour	1164:1190	The textile effluent colour was removed up to 50.35 and 54.95% using raw and modified clay, respectively.					
32502609	9	19	theme	synthetic	1334:1342	arg1	option					1410:1415	a viable option	1401:1415	a viable option for the treatment of industrial wastewater and textile effluents	1401:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	19	theme	synthetic	1334:1342	arg1	BFBN					1344:1347	synthetic BFBN	1334:1347	synthetic BFBN	1334:1347	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	19	theme	synthetic	1334:1342	arg1	dyes					1358:1361	RFRN dyes	1353:1361	RFRN dyes	1353:1361	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	20	theme	viable	1403:1408	arg1	option					1410:1415	a viable option	1401:1415	a viable option for the treatment of industrial wastewater and textile effluents	1401:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	20	theme	viable	1403:1408	arg1	BFBN					1344:1347	synthetic BFBN	1334:1347	synthetic BFBN	1334:1347	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	20	theme	viable	1403:1408	arg1	dyes					1358:1361	RFRN dyes	1353:1361	RFRN dyes	1353:1361	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	21	theme	promising	1295:1303	arg1	efficiency					1305:1314	promising efficiency	1295:1314	promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents	1295:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	0	22	theme	thermodynamic	103:115	arg1	studies					117:123	Kinetic, equilibrium and thermodynamic studies	78:123	Kinetic, equilibrium and thermodynamic studies	78:123	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	9	23	theme	modified	1274:1281	arg1	clay					1283:1286	The modified clay	1270:1286	The modified clay	1270:1286	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	24	theme	dyes	1358:1361	arg1	adsorption					1320:1329	adsorption	1320:1329	adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents	1320:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	3	25	dep	adsorbent	524:532	arg1	ions					543:546	ions	543:546	ions	543:546	The analysis showed a successful interaction between adsorbent and dyes ions.					
32502609	2	26	theme	diffraction	377:387	arg1	analyses					389:396	X-ray diffraction analyses	371:396	X-ray diffraction analyses	371:396	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	7	27	theme	adsorption	967:976	arg1	%					1033:1033	59.85%	1028:1033	59.85% for RFRN dye	1028:1046	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	7	27	theme	adsorption	967:976	arg1	%					1013:1013	76.39%	1008:1013	76.39% for BFBN	1008:1022	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	7	27	theme	adsorption	967:976	arg1	capacities					978:987	The maximum adsorption capacities	955:987	The maximum adsorption capacities	955:987	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	6	28	from	nature	947:952	arg1	exothermic					907:916	exothermic	907:916	exothermic	907:916	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	8	29	theme	modified	1241:1248	arg1	clay					1250:1253	modified clay	1241:1253	modified clay	1241:1253	The textile effluent colour was removed up to 50.35 and 54.95% using raw and modified clay, respectively.					
32502609	5	30	theme	pseudo-second-order	758:776	arg1	model					787:791	the pseudo-second-order kinetics model	754:791	the pseudo-second-order kinetics model	754:791	The findings revealed that at 80 min the adsorption grasped equilibrium in the case of both dyes and succeeded the pseudo-second-order kinetics model.					
32502609	2	31	theme	X-ray	371:375	arg1	analyses					389:396	X-ray diffraction analyses	371:396	X-ray diffraction analyses	371:396	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	5	32	theme	kinetics	778:785	arg1	model					787:791	the pseudo-second-order kinetics model	754:791	the pseudo-second-order kinetics model	754:791	The findings revealed that at 80 min the adsorption grasped equilibrium in the case of both dyes and succeeded the pseudo-second-order kinetics model.					
32502609	2	33	dep	Fourier	259:265	arg1	transform					267:275	transform	267:275	transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses	267:396	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	9	34	theme	BFBN	1344:1347	arg1	adsorption					1320:1329	adsorption	1320:1329	adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents	1320:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	9	35	theme	industrial	1438:1447	arg1	wastewater					1449:1458	industrial wastewater	1438:1458	industrial wastewater	1438:1458	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	1	36	theme	Blue	213:216	arg1	BFBN					223:226	BFBN	223:226	BFBN	223:226	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	1	36	theme	Blue	213:216	arg1	FBN					218:220	Blue FBN	213:220	Blue FBN (BFBN)	213:227	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	0	37	theme	acidified	37:45	arg1	clay					47:50	acidified clay	37:50	acidified clay for wastewater treatment	37:75	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	7	38	theme	RFRN	1039:1042	arg1	dye					1044:1046	RFRN dye	1039:1046	RFRN dye	1039:1046	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	9	39	theme	wastewater	1449:1458	arg1	treatment					1425:1433	the treatment	1421:1433	the treatment of industrial wastewater and textile effluents	1421:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	1	40	theme	FBN	218:220	arg1	elimination					198:208	the elimination	194:208	the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes	194:252	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	0	41	theme	wastewater	56:65	arg1	treatment					67:75	wastewater treatment	56:75	wastewater treatment	56:75	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	7	42	theme	higher	1102:1107	arg1	temperature					1109:1119	higher temperature	1102:1119	higher temperature	1102:1119	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	2	43	dep	transform	267:275	arg1	infrared					277:284	infrared	277:284	transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses	267:396	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	7	44	theme	maximum	959:965	arg1	%					1033:1033	59.85%	1028:1033	59.85% for RFRN dye	1028:1046	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	7	44	theme	maximum	959:965	arg1	%					1013:1013	76.39%	1008:1013	76.39% for BFBN	1008:1022	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	7	44	theme	maximum	959:965	arg1	capacities					978:987	The maximum adsorption capacities	955:987	The maximum adsorption capacities	955:987	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	7	45	dep	Composites	1069:1078	arg1	found					1080:1084	found	1080:1084	found to be stable at higher temperature	1080:1119	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	7	45	dep	Composites	1069:1078	arg1	regenerated					1125:1135	regenerated	1125:1135	regenerated using MgSO4 eluting agent	1125:1161	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	4	46	dep	fitted	580:585	arg1	RFRN					636:639	RFRN	636:639	RFRN	636:639	The experimental data was best fitted with Freundlich isotherm for both dyes (BFBN and RFRN).					
32502609	4	46	dep	fitted	580:585	arg1	BFBN					627:630	BFBN	627:630	BFBN	627:630	The experimental data was best fitted with Freundlich isotherm for both dyes (BFBN and RFRN).					
32502609	1	47	theme	Clay-based	126:135	arg1	composites					137:146	Clay-based composites	126:146	Clay-based composites	126:146	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	9	48	theme	effluents	1472:1480	arg1	treatment					1425:1433	the treatment	1421:1433	the treatment of industrial wastewater and textile effluents	1421:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	0	49	with	Biocomposite	0:11	arg1	clay					47:50	acidified clay	37:50	acidified clay for wastewater treatment	37:75	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	7	50	theme	MgSO4	1143:1147	arg1	agent					1157:1161	MgSO4 eluting agent	1143:1161	MgSO4 eluting agent	1143:1161	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	6	51	dep	changes	869:875	arg1	suggested					877:885	suggested	877:885	changes suggested that adsorption was exothermic, physical and spontaneous in nature	869:952	Furthermore, the enthalpy (ΔH°), Gibbs free energy (ΔG°) and entropy (ΔS°) changes suggested that adsorption was exothermic, physical and spontaneous in nature.					
32502609	1	52	dep	FBN	218:220	arg1	dyes					249:252	FRN (RFRN) dyes	238:252	FRN (RFRN) dyes	238:252	Clay-based composites were prepared, characterized, and applied for the elimination of Blue FBN (BFBN) and Rose FRN (RFRN) dyes.					
32502609	7	53	theme	eluting	1149:1155	arg1	agent					1157:1161	MgSO4 eluting agent	1143:1161	MgSO4 eluting agent	1143:1161	The maximum adsorption capacities were determined as 76.39% for BFBN and 59.85% for RFRN dye at pH 2.0 and 30 °C. Composites found to be stable at higher temperature and regenerated using MgSO4 eluting agent.					
32502609	4	54	theme	experimental	553:564	arg1	data					566:569	The experimental data	549:569	The experimental data	549:569	The experimental data was best fitted with Freundlich isotherm for both dyes (BFBN and RFRN).					
32502609	2	55	theme	molecule	445:452	arg1	interaction					426:436	the interaction	422:436	the interaction of dye molecule with adsorbents	422:468	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	4	56	theme	Freundlich	592:601	arg1	isotherm					603:610	Freundlich isotherm	592:610	Freundlich isotherm	592:610	The experimental data was best fitted with Freundlich isotherm for both dyes (BFBN and RFRN).					
32502609	9	57	from	adsorption	1320:1329	arg1	solution					1376:1383	aqueous solution	1368:1383	aqueous solution	1368:1383	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
32502609	8	58	theme	effluent	1176:1183	arg1	colour					1185:1190	The textile effluent colour	1164:1190	The textile effluent colour	1164:1190	The textile effluent colour was removed up to 50.35 and 54.95% using raw and modified clay, respectively.					
32502609	2	59	theme	dye	441:443	arg1	molecule					445:452	dye molecule	441:452	dye molecule	441:452	The Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Thermogravimetric (TGA) and X-ray diffraction analyses were performed to check the interaction of dye molecule with adsorbents.					
32502609	0	60	theme	sodium-alginate	16:30	arg1	sodium-alginate					16:30	sodium-alginate	16:30	sodium-alginate	16:30	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	0	60	theme	sodium-alginate	16:30	arg1	Biocomposite					0:11	Biocomposite	0:11	Biocomposite of sodium-alginate with acidified clay for wastewater treatment	0:75	Biocomposite of sodium-alginate with acidified clay for wastewater treatment: Kinetic, equilibrium and thermodynamic studies.					
32502609	5	61	theme	dyes	735:738	arg1	case					722:725	the case	718:725	the case of both dyes	718:738	The findings revealed that at 80 min the adsorption grasped equilibrium in the case of both dyes and succeeded the pseudo-second-order kinetics model.					
32502609	9	62	theme	textile	1464:1470	arg1	effluents					1472:1480	textile effluents	1464:1480	textile effluents	1464:1480	The modified clay showed promising efficiency for adsorption of synthetic BFBN and RFRN dyes from aqueous solution, which could be a viable option for the treatment of industrial wastewater and textile effluents.					
34801776	10	0	theme	metal	1681:1685	arg1	stress					1687:1692	metal stress	1681:1692	metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings	1681:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	6	1	theme	pectin	1205:1210	arg1	deposition					1157:1166	intensified deposition	1145:1166	intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin	1145:1248	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	2	2	theme	contamination	274:286	arg1	effects					257:263	the effects	253:263	the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes	253:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	6	3	theme	intensified	1145:1155	arg1	deposition					1157:1166	intensified deposition	1145:1166	intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin	1145:1248	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	6	4	theme	extensin	1241:1248	arg1	deposition					1157:1166	intensified deposition	1145:1166	intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin	1145:1248	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	3	5	dep	clay	542:545	arg1	NM					548:549	NM	548:549	NM	548:549	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	9	6	from	changes	1491:1497	arg1	composition					1529:1539	composition	1529:1539	composition	1529:1539	These changes in the apoplast structure and composition may have created actual barriers for the toxic ions.					
34801776	9	6	from	changes	1491:1497	arg1	structure					1515:1523	the apoplast structure	1502:1523	the apoplast structure	1502:1523	These changes in the apoplast structure and composition may have created actual barriers for the toxic ions.					
34801776	4	7	theme	Apoplast	646:653	arg1	reorganization					655:668	Apoplast reorganization	646:668	Apoplast reorganization in the nodules	646:683	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	6	8	theme	protein	1229:1235	arg1	deposition					1157:1166	intensified deposition	1145:1166	intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin	1145:1248	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	3	9	dep	NM	548:549	arg1	untreated					552:560	untreated	552:560	untreated	552:560	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	4	10	dep	protein	922:928	arg1	Jim12					952:956	Jim12	952:956	arabinogalactan protein - Lm14 and extensin - Jim12	906:956	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	4	10	dep	protein	922:928	arg1	extensin					941:948	extensin	941:948	extensin	941:948	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	4	10	dep	protein	922:928	arg1	Lm14					932:935	Lm14	932:935	Lm14	932:935	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	5	11	theme	apoplast	1030:1037	arg1	structure					1039:1047	apoplast structure	1030:1047	apoplast structure	1030:1047	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	10	12	theme	corniculatus-rhizobia	1735:1755	arg1	symbiosis					1757:1765	L. corniculatus-rhizobia symbiosis	1732:1765	L. corniculatus-rhizobia symbiosis	1732:1765	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	6	13	theme	arabinogalactan	1213:1227	arg1	protein					1229:1235	arabinogalactan protein	1213:1235	arabinogalactan protein	1213:1235	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	8	14	theme	cells	1440:1444	arg1	apoplast					1400:1407	the apoplast	1396:1407	the apoplast of cortex tissues and infected cells of Lotus nodules	1396:1461	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	7	15	theme	tissues	1350:1356	arg1	walls					1323:1327	the walls	1319:1327	the walls of the nodule cortex tissues	1319:1356	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	2	16	dep	apoplast	298:305	arg1	walls					313:317	cell walls	308:317	cell walls	308:317	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	2	16	dep	apoplast	298:305	arg1	spaces					337:342	intercellular spaces	323:342	intercellular spaces	323:342	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	10	17	theme	polluted	1776:1783	arg1	tailings					1794:1801	Zn-Pb polluted calamine tailings	1770:1801	Zn-Pb polluted calamine tailings	1770:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	8	18	theme	Lotus	1449:1453	arg1	nodules					1455:1461	Lotus nodules	1449:1461	Lotus nodules	1449:1461	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	7	19	theme	nodule	1336:1341	arg1	tissues					1350:1356	the nodule cortex tissues	1332:1356	the nodule cortex tissues	1332:1356	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	4	20	theme	specific	704:711	arg1	dyes					713:716	specific dyes	704:716	specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12)	704:957	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	1	21	theme	symbiotic	127:135	arg1	fixation					146:153	symbiotic nitrogen fixation	127:153	symbiotic nitrogen fixation	127:153	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	1	21	theme	symbiotic	127:135	arg1	factors					169:175	important factors	159:175	important factors that determine legume growth	159:204	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	1	21	theme	symbiotic	127:135	arg1	Nodulation					112:121	Nodulation	112:121	Nodulation	112:121	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	5	22	from	changes	1015:1021	arg1	structure					1039:1047	apoplast structure	1030:1047	apoplast structure	1030:1047	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	5	22	from	changes	1015:1021	arg1	composition					1053:1063	composition	1053:1063	composition	1053:1063	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	5	23	theme	cells	1103:1107	arg1	structure					1039:1047	apoplast structure	1030:1047	apoplast structure	1030:1047	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	5	23	theme	cells	1103:1107	arg1	composition					1053:1063	composition	1053:1063	composition	1053:1063	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	1	24	theme	important	159:167	arg1	fixation					146:153	symbiotic nitrogen fixation	127:153	symbiotic nitrogen fixation	127:153	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	1	24	theme	important	159:167	arg1	factors					169:175	important factors	159:175	important factors that determine legume growth	159:204	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	1	24	theme	important	159:167	arg1	Nodulation					112:121	Nodulation	112:121	Nodulation	112:121	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	4	25	theme	arabinogalactan	906:920	arg1	protein					922:928	arabinogalactan protein	906:928	arabinogalactan protein - Lm14 and extensin - Jim12	906:956	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	6	26	theme	xyloglucan	1182:1191	arg1	deposition					1157:1166	intensified deposition	1145:1166	intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin	1145:1248	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	0	27	theme	old	85:87	arg1	wastes					104:109	old Zn-Pb calamine wastes	85:109	old Zn-Pb calamine wastes	85:109	Exploring apoplast reorganization in the nodules of Lotus corniculatus L. growing on old Zn-Pb calamine wastes.					
34801776	0	28	theme	calamine	95:102	arg1	wastes					104:109	old Zn-Pb calamine wastes	85:109	old Zn-Pb calamine wastes	85:109	Exploring apoplast reorganization in the nodules of Lotus corniculatus L. growing on old Zn-Pb calamine wastes.					
34801776	5	29	theme	cortex	1075:1080	arg1	tissues					1082:1088	nodule cortex tissues	1068:1088	nodule cortex tissues	1068:1088	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	2	30	theme	calamine	425:432	arg1	wastes					434:439	old calamine wastes	421:439	old calamine wastes	421:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	2	31	theme	trefoil	360:366	arg1	apoplast					298:305	nodule apoplast	291:305	nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes	291:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	9	32	theme	actual	1558:1563	arg1	barriers					1565:1572	actual barriers	1558:1572	actual barriers for the toxic ions	1558:1591	These changes in the apoplast structure and composition may have created actual barriers for the toxic ions.					
34801776	2	33	from	effects	257:263	arg1	apoplast					298:305	nodule apoplast	291:305	nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes	291:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	7	34	theme	suberin	1297:1303	arg1	deposition					1305:1314	suberin deposition	1297:1314	suberin deposition	1297:1314	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	3	35	theme	Lotus-nodulating	600:615	arg1	liaoningense					632:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	4	36	dep	pectins	856:862	arg1	Jim7					874:877	Jim7	874:877	Jim7	874:877	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	4	36	dep	pectins	856:862	arg1	Jim5					865:868	Jim5	865:868	Jim5	865:868	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	4	36	dep	pectins	856:862	arg1	pectins					856:862	pectins	856:862	pectins (Jim5 and Jim7)	856:878	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	9	37	theme	toxic	1582:1586	arg1	ions					1588:1591	the toxic ions	1578:1591	the toxic ions	1578:1591	These changes in the apoplast structure and composition may have created actual barriers for the toxic ions.					
34801776	5	38	theme	nodules	998:1004	arg1	analysis					972:979	Microscopic analysis	960:979	Microscopic analysis of metal-treated nodules	960:1004	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	2	39	dep	trefoil	360:366	arg1	L.					388:389	Lotus corniculatus L.	369:389	Lotus corniculatus L.	369:389	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	10	40	theme	defense	1656:1662	arg1	strategy					1664:1671	legume defense strategy	1649:1671	legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings	1649:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	7	41	from	deposition	1305:1314	arg1	walls					1323:1327	the walls	1319:1327	the walls of the nodule cortex tissues	1319:1356	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	7	42	theme	metal	1255:1259	arg1	presence					1261:1268	The metal presence	1251:1268	The metal presence	1251:1268	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	8	43	theme	tissues	1419:1425	arg1	apoplast					1400:1407	the apoplast	1396:1407	the apoplast of cortex tissues and infected cells of Lotus nodules	1396:1461	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	3	44	theme	sterile	484:490	arg1	substrate					501:509	sterile calamine substrate	484:509	sterile calamine substrate (M, metal treated)	484:528	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	5	45	theme	Microscopic	960:970	arg1	analysis					972:979	Microscopic analysis	960:979	Microscopic analysis of metal-treated nodules	960:1004	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	5	46	dep	structure	1039:1047	arg1	the					1026:1028	the	1026:1028	the	1026:1028	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	3	47	link	calamine-derived	583:598	arg1	liaoningense					632:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	10	48	theme	L.	1732:1733	arg1	symbiosis					1757:1765	L. corniculatus-rhizobia symbiosis	1732:1765	L. corniculatus-rhizobia symbiosis	1732:1765	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	4	49	from	reorganization	655:668	arg1	nodules					677:683	the nodules	673:683	the nodules	673:683	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	10	50	theme	Zn-Pb	1770:1774	arg1	tailings					1794:1801	Zn-Pb polluted calamine tailings	1770:1801	Zn-Pb polluted calamine tailings	1770:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	2	51	theme	Zn-Pb	268:272	arg1	contamination					274:286	Zn-Pb contamination	268:286	Zn-Pb contamination	268:286	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	2	52	theme	cell	308:311	arg1	walls					313:317	cell walls	308:317	cell walls	308:317	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	10	53	theme	calamine	1785:1792	arg1	tailings					1794:1801	Zn-Pb polluted calamine tailings	1770:1801	Zn-Pb polluted calamine tailings	1770:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	10	54	theme	symbiosis	1757:1765	arg1	effective					1707:1715	effective	1707:1715	effective	1707:1715	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	8	55	theme	infected	1431:1438	arg1	cells					1440:1444	infected cells	1431:1444	infected cells	1431:1444	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	6	56	theme	Wall	1110:1113	arg1	thickening					1115:1124	Wall thickening	1110:1124	Wall thickening	1110:1124	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	10	57	theme	strategy	1664:1671	arg1	they					1611:1614	they	1611:1614	they	1611:1614	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	10	57	theme	strategy	1664:1671	arg1	element					1638:1644	an element	1635:1644	an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings	1635:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	1	58	theme	legume	192:197	arg1	growth					199:204	legume growth	192:204	legume growth	192:204	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	4	59	theme	structural	885:894	arg1	proteins					896:903	structural proteins	885:903	structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12)	885:957	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	4	60	dep	proteins	896:903	arg1	protein					922:928	arabinogalactan protein	906:928	arabinogalactan protein - Lm14 and extensin - Jim12	906:956	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	3	61	theme	expanded	533:540	arg1	clay					542:545	expanded clay	533:545	expanded clay (NM, untreated)	533:561	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	3	62	dep	substrate	501:509	arg1	M					512:512	M	512:512	M	512:512	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	3	62	dep	substrate	501:509	arg1	metal					515:519	metal	515:519	metal	515:519	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	8	63	theme	nodules	1455:1461	arg1	cells					1440:1444	infected cells	1431:1444	infected cells	1431:1444	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	8	63	theme	nodules	1455:1461	arg1	tissues					1419:1425	cortex tissues	1412:1425	cortex tissues	1412:1425	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	6	64	theme	esterified	1194:1203	arg1	pectin					1205:1210	esterified pectin	1194:1210	esterified pectin	1194:1210	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	8	65	theme	Zn-Pb	1469:1473	arg1	presence					1475:1482	Zn-Pb presence	1469:1482	Zn-Pb presence	1469:1482	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	7	66	theme	cortex	1343:1348	arg1	tissues					1350:1356	the nodule cortex tissues	1332:1356	the nodule cortex tissues	1332:1356	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	0	67	theme	L.	71:72	arg1	nodules					41:47	the nodules	37:47	the nodules of Lotus corniculatus L. growing on old Zn-Pb calamine wastes	37:109	Exploring apoplast reorganization in the nodules of Lotus corniculatus L. growing on old Zn-Pb calamine wastes.					
34801776	1	68	theme	nitrogen	137:144	arg1	fixation					146:153	symbiotic nitrogen fixation	127:153	symbiotic nitrogen fixation	127:153	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	1	68	theme	nitrogen	137:144	arg1	factors					169:175	important factors	159:175	important factors that determine legume growth	159:204	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	1	68	theme	nitrogen	137:144	arg1	Nodulation					112:121	Nodulation	112:121	Nodulation	112:121	Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth.					
34801776	6	69	theme	cellulose	1171:1179	arg1	deposition					1157:1166	intensified deposition	1145:1166	intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin	1145:1248	Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin.					
34801776	5	70	theme	infected	1094:1101	arg1	cells					1103:1107	infected cells	1094:1107	infected cells	1094:1107	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	9	71	theme	apoplast	1506:1513	arg1	structure					1515:1523	the apoplast structure	1502:1523	the apoplast structure	1502:1523	These changes in the apoplast structure and composition may have created actual barriers for the toxic ions.					
34801776	0	72	theme	Zn-Pb	89:93	arg1	wastes					104:109	old Zn-Pb calamine wastes	85:109	old Zn-Pb calamine wastes	85:109	Exploring apoplast reorganization in the nodules of Lotus corniculatus L. growing on old Zn-Pb calamine wastes.					
34801776	2	73	theme	nodule	291:296	arg1	apoplast					298:305	nodule apoplast	291:305	nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes	291:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	5	74	theme	nodule	1068:1073	arg1	tissues					1082:1088	nodule cortex tissues	1068:1088	nodule cortex tissues	1068:1088	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	4	75	theme	lignin	744:749	arg1	detection					751:759	lignin detection	744:759	lignin detection	744:759	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	0	76	theme	apoplast	10:17	arg1	reorganization					19:32	apoplast reorganization	10:32	apoplast reorganization	10:32	Exploring apoplast reorganization in the nodules of Lotus corniculatus L. growing on old Zn-Pb calamine wastes.					
34801776	5	77	theme	tissues	1082:1088	arg1	structure					1039:1047	apoplast structure	1030:1047	apoplast structure	1030:1047	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	5	77	theme	tissues	1082:1088	arg1	composition					1053:1063	composition	1053:1063	composition	1053:1063	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	2	78	theme	old	421:423	arg1	wastes					434:439	old calamine wastes	421:439	old calamine wastes	421:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	2	79	theme	foot	355:358	arg1	trefoil					360:366	bird's foot trefoil	348:366	bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes	348:439	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	3	80	theme	calamine-derived	583:598	arg1	liaoningense					632:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	5	81	theme	metal-treated	984:996	arg1	nodules					998:1004	metal-treated nodules	984:1004	metal-treated nodules	984:1004	Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells.					
34801776	7	82	from	lignin	1286:1291	arg1	walls					1323:1327	the walls	1319:1327	the walls of the nodule cortex tissues	1319:1356	The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues.					
34801776	2	83	theme	pot	209:211	arg1	experiment					213:222	A pot experiment	207:222	A pot experiment	207:222	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	4	84	theme	monoclonal	807:816	arg1	antibodies					818:827	monoclonal antibodies	807:827	monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12)	807:957	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	2	85	theme	intercellular	323:335	arg1	spaces					337:342	intercellular spaces	323:342	intercellular spaces	323:342	A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes.					
34801776	4	86	theme	immuno-histochemical	766:785	arg1	techniques					787:796	immuno-histochemical techniques	766:796	immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12)	766:957	Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein - Lm14 and extensin - Jim12).					
34801776	3	87	theme	Bradyrhizobium	617:630	arg1	liaoningense					632:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	calamine-derived Lotus-nodulating Bradyrhizobium liaoningense	583:643	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34801776	8	88	theme	apoplast	1400:1407	arg1	reorganization					1378:1391	reorganization	1378:1391	reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules	1378:1461	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	10	89	theme	legume	1649:1654	arg1	strategy					1664:1671	legume defense strategy	1649:1671	legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings	1649:1801	For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.					
34801776	8	90	theme	cortex	1412:1417	arg1	tissues					1419:1425	cortex tissues	1412:1425	cortex tissues	1412:1425	Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence.					
34801776	3	91	theme	calamine	492:499	arg1	substrate					501:509	sterile calamine substrate	484:509	sterile calamine substrate (M, metal treated)	484:528	The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense.					
34624763	6	0	theme	slow	1223:1226	arg1	rate					1240:1243	the slow dissolution rate	1219:1243	the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions	1219:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	4	1	from	ions	891:894	arg1	cell					952:955	the microbial cell	938:955	the microbial cell	938:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	1	2	theme	nanowire	163:170	arg1	hypromellose					198:209	copper nanowire impregnated biocompatible hypromellose	156:209	copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	156:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	6	3	theme	production	1261:1270	arg1	rate					1240:1243	the slow dissolution rate	1219:1243	the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions	1219:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	5	4	theme	dermal	1032:1037	arg1	fibroblast					1039:1048	normal human dermal fibroblast	1019:1048	normal human dermal fibroblast at minimum bactericidal concentration (MBC)	1019:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	7	5	theme	strong	1344:1349	arg1	potential					1351:1359	a strong potential	1342:1359	a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications	1342:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	6	6	dep	film	1252:1255	arg1	the					1248:1250	the	1248:1250	the	1248:1250	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	1	7	theme	impregnated	172:182	arg1	hypromellose					198:209	copper nanowire impregnated biocompatible hypromellose	156:209	copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	156:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	6	8	theme	copper	1111:1116	arg1	nanoparticles					1118:1130	the copper nanoparticles	1107:1130	the copper nanoparticles reported earlier in vitro studies	1107:1164	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	7	9	theme	clinical	1430:1437	arg1	applications					1439:1450	broader biomedical and clinical applications	1407:1450	broader biomedical and clinical applications	1407:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	4	10	theme	antibacterial	757:769	arg1	mechanism					771:779	the antibacterial mechanism	753:779	the antibacterial mechanism of the nanocomposite	753:800	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	3	11	theme	4.8 wt	626:631	arg1	loading					643:649	4.8 wt% nanowire loading	626:649	4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus	626:718	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	4	12	theme	membrane	849:856	arg1	damage					858:863	cellular membrane damage	840:863	cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell	840:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	5	13	theme	minimum	1053:1059	arg1	concentration					1074:1086	minimum bactericidal concentration	1053:1086	minimum bactericidal concentration (MBC)	1053:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	5	13	theme	minimum	1053:1059	arg1	MBC					1089:1091	MBC	1089:1091	MBC	1089:1091	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	3	14	theme	%	632:632	arg1	loading					643:649	4.8 wt% nanowire loading	626:649	4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus	626:718	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	2	15	theme	infrared	341:348	arg1	spectroscopy					350:361	infrared spectroscopy	341:361	infrared spectroscopy	341:361	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	4	16	theme	multiple	812:819	arg1	damage					858:863	cellular membrane damage	840:863	cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell	840:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	4	16	theme	multiple	812:819	arg1	pathways					821:828	multiple pathways	812:828	multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell	812:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	2	17	theme	uniform	418:424	arg1	dispersion					426:435	uniform dispersion	418:435	uniform dispersion of copper nanowire in the polymer matrix	418:476	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	5	18	theme	bactericidal	1061:1072	arg1	concentration					1074:1086	minimum bactericidal concentration	1053:1086	minimum bactericidal concentration (MBC)	1053:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	5	18	theme	bactericidal	1061:1072	arg1	MBC					1089:1091	MBC	1089:1091	MBC	1089:1091	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	3	19	theme	excellent	541:549	arg1	activity					565:572	excellent antibacterial activity	541:572	excellent antibacterial activity against both Gram positive and negative bacteria	541:621	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	4	20	theme	copper	884:889	arg1	ions					891:894	released copper ions	875:894	released copper ions	875:894	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	1	21	theme	biocompatible	184:196	arg1	hypromellose					198:209	copper nanowire impregnated biocompatible hypromellose	156:209	copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	156:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	5	22	theme	normal	1019:1024	arg1	fibroblast					1039:1048	normal human dermal fibroblast	1019:1048	normal human dermal fibroblast at minimum bactericidal concentration (MBC)	1019:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	4	23	theme	oxygen	909:914	arg1	species					916:922	reactive oxygen species	900:922	reactive oxygen species generation	900:933	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	6	24	theme	film	1252:1255	arg1	rate					1240:1243	the slow dissolution rate	1219:1243	the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions	1219:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	1	25	theme	present	80:86	arg1	work					88:91	The present work	76:91	The present work	76:91	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	2	26	theme	polymer	463:469	arg1	matrix					471:476	the polymer matrix	459:476	the polymer matrix	459:476	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	0	27	theme	nanowire	7:14	arg1	hypromellose					25:36	Copper nanowire embedded hypromellose	0:36	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.	0:74	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.					
34624763	3	28	theme	negative	605:612	arg1	bacteria					614:621	both Gram positive and negative bacteria	582:621	both Gram positive and negative bacteria	582:621	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	2	29	from	dispersion	426:435	arg1	matrix					471:476	the polymer matrix	459:476	the polymer matrix	459:476	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	4	30	theme	cellular	840:847	arg1	damage					858:863	cellular membrane damage	840:863	cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell	840:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	7	31	theme	biomedical	1415:1424	arg1	applications					1439:1450	broader biomedical and clinical applications	1407:1450	broader biomedical and clinical applications	1407:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	0	32	theme	Copper	0:5	arg1	hypromellose					25:36	Copper nanowire embedded hypromellose	0:36	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.	0:74	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.					
34624763	3	33	theme	MIC	656:658	arg1	values					660:665	MIC values	656:665	MIC values of 400 µg/mL and 500 µg/mL	656:692	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	1	34	theme	polyethylene	217:228	arg1	glycol					230:235	polyethylene glycol	217:235	polyethylene glycol	217:235	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	1	34	theme	polyethylene	217:228	arg1	plasticiser					242:252	a plasticiser	240:252	a plasticiser	240:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	3	35	theme	500 µg/mL	684:692	arg1	values					660:665	MIC values	656:665	MIC values of 400 µg/mL and 500 µg/mL	656:692	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	2	36	theme	UV-Visible	364:373	arg1	spectroscopy					375:386	UV-Visible spectroscopy	364:386	UV-Visible spectroscopy	364:386	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	7	37	dep	biomedical	1415:1424	arg1	broader					1407:1413	broader	1407:1413	broader	1407:1413	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	6	38	theme	low	1172:1174	arg1	cytotoxicity					1176:1187	this low cytotoxicity	1167:1187	this low cytotoxicity of copper nanowires	1167:1207	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	0	39	theme	embedded	16:23	arg1	hypromellose					25:36	Copper nanowire embedded hypromellose	0:36	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.	0:74	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.					
34624763	4	40	from	generation	924:933	arg1	cell					952:955	the microbial cell	938:955	the microbial cell	938:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	1	41	theme	novel	103:107	arg1	film					137:140	a novel antibacterial nanocomposite film	101:140	a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	101:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	2	42	theme	apparent	490:497	arg1	oxidation					499:507	any apparent oxidation	486:507	any apparent oxidation	486:507	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	4	43	theme	nanocomposite	788:800	arg1	mechanism					771:779	the antibacterial mechanism	753:779	the antibacterial mechanism of the nanocomposite	753:800	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	3	44	with	loading	643:649	arg1	values					660:665	MIC values	656:665	MIC values of 400 µg/mL and 500 µg/mL	656:692	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	3	45	theme	400 µg/mL	670:678	arg1	values					660:665	MIC values	656:665	MIC values of 400 µg/mL and 500 µg/mL	656:692	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	7	46	theme	composites	1387:1396	arg1	films					1398:1402	copper nanowire-based composites films	1365:1402	copper nanowire-based composites films in broader biomedical and clinical applications	1365:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	6	47	theme	nanowires	1199:1207	arg1	cytotoxicity					1176:1187	this low cytotoxicity	1167:1187	this low cytotoxicity of copper nanowires	1167:1207	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	6	48	theme	amount	1281:1286	arg1	production					1261:1270	production	1261:1270	production	1261:1270	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	6	48	theme	amount	1281:1286	arg1	film					1252:1255	film	1252:1255	film	1252:1255	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	2	49	theme	physico-chemical	264:279	arg1	characterization					281:296	Detailed physico-chemical characterization	255:296	Detailed physico-chemical characterization	255:296	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	0	50	theme	nanocomposite	56:68	arg1	film					70:73	An antibacterial nanocomposite film	39:73	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.	0:74	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.					
34624763	5	51	theme	good	989:992	arg1	biocompatibility					994:1009	good biocompatibility	989:1009	good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC)	989:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	7	52	theme	copper	1365:1370	arg1	films					1398:1402	copper nanowire-based composites films	1365:1402	copper nanowire-based composites films in broader biomedical and clinical applications	1365:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	2	53	theme	Detailed	255:262	arg1	characterization					281:296	Detailed physico-chemical characterization	255:296	Detailed physico-chemical characterization	255:296	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	7	54	from	films	1398:1402	arg1	applications					1439:1450	broader biomedical and clinical applications	1407:1450	broader biomedical and clinical applications	1407:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
34624763	1	55	theme	antibacterial	109:121	arg1	film					137:140	a novel antibacterial nanocomposite film	101:140	a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	101:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	0	56	theme	antibacterial	42:54	arg1	film					70:73	An antibacterial nanocomposite film	39:73	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.	0:74	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.					
34624763	4	57	theme	microbial	942:950	arg1	cell					952:955	the microbial cell	938:955	the microbial cell	938:955	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	2	58	dep	transform	331:339	arg1	shows					412:416	shows	412:416	transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation	331:507	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	1	59	theme	copper	156:161	arg1	hypromellose					198:209	copper nanowire impregnated biocompatible hypromellose	156:209	copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	156:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	3	60	theme	positive	592:599	arg1	bacteria					614:621	both Gram positive and negative bacteria	582:621	both Gram positive and negative bacteria	582:621	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	1	61	theme	nanocomposite	123:135	arg1	film					137:140	a novel antibacterial nanocomposite film	101:140	a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser	101:252	The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser.					
34624763	5	62	from	concentration	1074:1086	arg1	fibroblast					1039:1048	normal human dermal fibroblast	1019:1048	normal human dermal fibroblast at minimum bactericidal concentration (MBC)	1019:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	6	63	theme	ROS	1291:1293	arg1	ROS					1291:1293	ROS	1291:1293	ROS producing Cu2+ ions	1291:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	6	63	theme	ROS	1291:1293	arg1	amount					1281:1286	lower amount	1275:1286	lower amount of ROS producing Cu2+ ions	1275:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	6	64	theme	lower	1275:1279	arg1	ROS					1291:1293	ROS	1291:1293	ROS producing Cu2+ ions	1291:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	6	64	theme	lower	1275:1279	arg1	amount					1281:1286	lower amount	1275:1286	lower amount of ROS producing Cu2+ ions	1275:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	0	65	dep	hypromellose	25:36	arg1	film					70:73	An antibacterial nanocomposite film	39:73	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.	0:74	Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.					
34624763	4	66	theme	species	916:922	arg1	generation					924:933	reactive oxygen species generation	900:933	reactive oxygen species generation	900:933	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	3	67	theme	antibacterial	551:563	arg1	activity					565:572	excellent antibacterial activity	541:572	excellent antibacterial activity against both Gram positive and negative bacteria	541:621	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	3	68	theme	nanowire	634:641	arg1	loading					643:649	4.8 wt% nanowire loading	626:649	4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus	626:718	The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively.					
34624763	6	69	theme	vitro	1152:1156	arg1	studies					1158:1164	vitro studies	1152:1164	vitro studies	1152:1164	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	4	70	theme	released	875:882	arg1	ions					891:894	released copper ions	875:894	released copper ions	875:894	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	5	71	theme	human	1026:1030	arg1	fibroblast					1039:1048	normal human dermal fibroblast	1019:1048	normal human dermal fibroblast at minimum bactericidal concentration (MBC)	1019:1092	Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC).					
34624763	6	72	theme	Cu2+	1305:1308	arg1	ions					1310:1313	Cu2+ ions	1305:1313	Cu2+ ions	1305:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	2	73	theme	electron	392:399	arg1	microscopy					401:410	electron microscopy	392:410	electron microscopy	392:410	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	6	74	theme	dissolution	1228:1238	arg1	rate					1240:1243	the slow dissolution rate	1219:1243	the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions	1219:1313	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	2	75	theme	X-ray	304:308	arg1	diffraction					310:320	X-ray diffraction	304:320	X-ray diffraction	304:320	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	2	76	theme	nanowire	447:454	arg1	dispersion					426:435	uniform dispersion	418:435	uniform dispersion of copper nanowire in the polymer matrix	418:476	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	6	77	theme	copper	1192:1197	arg1	nanowires					1199:1207	copper nanowires	1192:1207	copper nanowires	1192:1207	Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions.					
34624763	4	78	theme	reactive	900:907	arg1	species					916:922	reactive oxygen species	900:922	reactive oxygen species generation	900:933	Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell.					
34624763	2	79	theme	copper	440:445	arg1	nanowire					447:454	copper nanowire	440:454	copper nanowire	440:454	Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation.					
34624763	7	80	theme	nanowire-based	1372:1385	arg1	films					1398:1402	copper nanowire-based composites films	1365:1402	copper nanowire-based composites films in broader biomedical and clinical applications	1365:1450	Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.					
32751335	10	0	dep	11.6	1740:1743	arg1	to					1737:1738	to	1737:1738	to	1737:1738	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	9	1	contain	containing	1481:1490	arg2	nanofiller					1511:1520	the nanofiller	1507:1520	the nanofiller	1507:1520	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	1	contain	containing	1481:1490	arg2	%					1502:1502	only 2 wt %	1492:1502	only 2 wt % of the nanofiller	1492:1520	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	1	contain	containing	1481:1490	arg1	samples					1473:1479	the paper samples	1463:1479	the paper samples containing only 2 wt % of the nanofiller	1463:1520	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	3	2	theme	fibers	648:653	arg1	functionalization					613:629	the successful functionalization	598:629	the successful functionalization of the cellulose fibers with the nanomaterial	598:675	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	3	theme	atomic	544:549	arg1	microscopy					557:566	atomic force microscopy	544:566	atomic force microscopy (AFM)	544:572	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	3	theme	atomic	544:549	arg1	AFM					569:571	AFM	569:571	AFM	569:571	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	6	4	theme	working	1086:1092	arg1	suspension					1104:1113	working bacterial suspension	1086:1113	working bacterial suspension	1086:1113	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	6	5	theme	volume	1076:1081	arg1	influence					1059:1067	the influence	1055:1067	the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials	1055:1171	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	3	6	theme	microscopic	517:527	arg1	results					583:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results	481:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results	481:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	4	7	theme	wt	807:808	arg1	%					810:810	2 wt %	805:810	2 wt %	805:810	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	6	8	theme	obtained	1154:1161	arg1	materials					1163:1171	the obtained materials	1150:1171	the obtained materials	1150:1171	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	9	9	theme	Tensile	1402:1408	arg1	strength					1410:1417	Tensile strength	1402:1417	Tensile strength	1402:1417	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	10	theme	control	1579:1585	arg1	samples					1593:1599	the control paper samples	1575:1599	the control paper samples	1575:1599	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	3	11	theme	force	551:555	arg1	microscopy					557:566	atomic force microscopy	544:566	atomic force microscopy (AFM)	544:572	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	11	theme	force	551:555	arg1	AFM					569:571	AFM	569:571	AFM	569:571	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	10	12	theme	thermal	1657:1663	arg1	properties					1665:1674	the thermal properties	1653:1674	the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%	1653:1744	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	10	13	theme	heat	1703:1706	arg1	rate					1716:1719	the composite paper-the heat release rate	1679:1719	the composite paper-the heat release rate decreased by up to 11.6%	1679:1744	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	4	14	dep	nanomaterial	783:794	arg1	%					802:802	1 wt %	797:802	1 wt %	797:802	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	4	14	dep	nanomaterial	783:794	arg1	%					822:822	3 wt %	817:822	3 wt %	817:822	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	4	14	dep	nanomaterial	783:794	arg1	%					810:810	2 wt %	805:810	2 wt %	805:810	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	4	15	theme	Modified	678:685	arg1	fibers					697:702	Modified cellulose fibers	678:702	Modified cellulose fibers	678:702	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	10	16	theme	composite	1683:1691	arg1	rate					1716:1719	the composite paper-the heat release rate	1679:1719	the composite paper-the heat release rate decreased by up to 11.6%	1679:1744	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	3	17	dep	microscopic	517:527	arg1	microscopy					557:566	atomic force microscopy	544:566	atomic force microscopy (AFM)	544:572	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	17	dep	microscopic	517:527	arg1	SEM					535:537	SEM	535:537	SEM	535:537	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	17	dep	microscopic	517:527	arg1	TEM					530:532	TEM	530:532	TEM	530:532	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	17	dep	microscopic	517:527	arg1	AFM					569:571	AFM	569:571	AFM	569:571	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	18	theme	methods	575:581	arg1	results					583:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results	481:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results	481:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	7	19	theme	bacterial	1272:1280	arg1	suspension					1282:1291	bacterial suspension	1272:1291	bacterial suspension	1272:1291	The results showed significantly better antibacterial performance when the volume of bacterial suspension was reduced.					
32751335	0	20	theme	Paper	59:63	arg1	h-BN					21:24	h-BN	21:24	h-BN as Nanofiller of Cellulose-Based Paper	21:63	Few Layered Oxidized h-BN as Nanofiller of Cellulose-Based Paper with Superior Antibacterial Response and Enhanced Mechanical/Thermal Performance.					
32751335	5	21	theme	model	1025:1029	arg1	bacteria					1031:1038	both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria	961:1038	bacteria	1031:1038	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	10	22	theme	rate	1716:1719	arg1	properties					1665:1674	the thermal properties	1653:1674	the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%	1653:1744	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	0	23	theme	Superior	70:77	arg1	Response					93:100	Superior Antibacterial Response	70:100	Superior Antibacterial Response	70:100	Few Layered Oxidized h-BN as Nanofiller of Cellulose-Based Paper with Superior Antibacterial Response and Enhanced Mechanical/Thermal Performance.					
32751335	1	24	theme	boron	172:176	arg1	nanosheets					186:195	hexagonal boron nitride nanosheets	162:195	hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH)	162:235	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	4	25	theme	sheets	731:736	arg1	samples					738:744	paper sheets samples	725:744	paper sheets samples	725:744	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	9	26	theme	nanofiller	1511:1520	arg1	%					1502:1502	only 2 wt %	1492:1502	only 2 wt % of the nanofiller	1492:1520	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	26	theme	nanofiller	1511:1520	arg1	nanofiller					1511:1520	the nanofiller	1507:1520	the nanofiller	1507:1520	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	1	27	theme	h-BN	363:366	arg1	oxidation					345:353	oxidation	345:353	oxidation	345:353	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	1	27	theme	h-BN	363:366	arg1	exfoliation					329:339	exfoliation	329:339	exfoliation	329:339	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	1	28	dep	exfoliation	329:339	arg1	the					304:306	the	304:306	the	304:306	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	0	29	theme	Enhanced	106:113	arg1	Performance					134:144	Enhanced Mechanical/Thermal Performance	106:144	Enhanced Mechanical/Thermal Performance	106:144	Few Layered Oxidized h-BN as Nanofiller of Cellulose-Based Paper with Superior Antibacterial Response and Enhanced Mechanical/Thermal Performance.					
32751335	2	30	theme	fibers	441:446	arg1	surface					420:426	the surface	416:426	the surface of cellulose fibers	416:446	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	8	31	theme	Mechanical	1306:1315	arg1	properties					1317:1326	Mechanical properties	1306:1326	Mechanical properties of the paper samples with and without nanofiller	1306:1375	Mechanical properties of the paper samples with and without nanofiller were also characterized.					
32751335	2	32	theme	h-BN-OH	382:388	arg1	h-BN-OH					382:388	h-BN-OH	382:388	h-BN-OH	382:388	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	2	32	theme	h-BN-OH	382:388	arg1	ones					398:401	the ones	394:401	the ones presented on the surface of cellulose fibers	394:446	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	2	32	theme	h-BN-OH	382:388	arg1	groups					372:377	OH groups	369:377	OH groups of h-BN-OH	369:388	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	1	33	theme	cellulose	281:289	arg1	fibers					291:296	cellulose fibers	281:296	cellulose fibers	281:296	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	6	34	theme	suspension	1104:1113	arg1	volume					1076:1081	the volume	1072:1081	the volume of working bacterial suspension	1072:1113	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	5	35	theme	antibacterial	862:874	arg1	properties					876:885	the antibacterial properties	858:885	the antibacterial properties	858:885	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	5	36	dep	bacteria	1031:1038	arg1	epidermidis					1012:1022	S. epidermidis	1009:1022	S. epidermidis	1009:1022	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	3	37	theme	successful	602:611	arg1	functionalization					613:629	the successful functionalization	598:629	the successful functionalization of the cellulose fibers with the nanomaterial	598:675	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	8	38	theme	paper	1335:1339	arg1	samples					1341:1347	the paper samples	1331:1347	the paper samples	1331:1347	Mechanical properties of the paper samples with and without nanofiller were also characterized.					
32751335	6	39	from	influence	1059:1067	arg1	efficiency					1136:1145	the antibacterial efficiency	1118:1145	the antibacterial efficiency of the obtained materials	1118:1171	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	1	40	theme	simple	308:313	arg1	exfoliation					329:339	exfoliation	329:339	exfoliation	329:339	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	5	41	theme	good	936:939	arg1	performance					941:951	good performance	936:951	good performance	936:951	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	7	42	theme	better	1220:1225	arg1	performance					1241:1251	significantly better antibacterial performance	1206:1251	significantly better antibacterial performance	1206:1251	The results showed significantly better antibacterial performance when the volume of bacterial suspension was reduced.					
32751335	4	43	theme	wt	799:800	arg1	%					802:802	1 wt %	797:802	1 wt %	797:802	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	3	44	theme	spectroscopic	486:498	arg1	results					583:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results	481:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results	481:589	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	1	45	theme	effective	319:327	arg1	exfoliation					329:339	exfoliation	329:339	exfoliation	329:339	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	6	46	theme	bacterial	1094:1102	arg1	suspension					1104:1113	working bacterial suspension	1086:1113	working bacterial suspension	1086:1113	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	3	47	theme	cellulose	638:646	arg1	fibers					648:653	the cellulose fibers	634:653	the cellulose fibers with the nanomaterial	634:675	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	11	48	theme	paper	1867:1871	arg1	coatings					1873:1880	paper coatings	1867:1880	paper coatings	1867:1880	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	5	49	theme	unit	910:913	arg1	method					915:920	unit method	910:920	unit method	910:920	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	4	50	used	used	709:712	arg2	fibers					697:702	Modified cellulose fibers	678:702	Modified cellulose fibers	678:702	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	9	51	theme	paper	1467:1471	arg1	samples					1473:1479	the paper samples	1463:1479	the paper samples containing only 2 wt % of the nanofiller	1463:1520	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	5	52	dep	Gram-negative	966:978	arg1	coli					984:987	E. coli	981:987	E. coli	981:987	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	1	53	theme	nitride	178:184	arg1	nanosheets					186:195	hexagonal boron nitride nanosheets	162:195	hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH)	162:235	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	6	54	theme	materials	1163:1171	arg1	efficiency					1136:1145	the antibacterial efficiency	1118:1145	the antibacterial efficiency of the obtained materials	1118:1171	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	1	55	theme	hydroxyl	211:218	arg1	groups					220:225	hydroxyl groups	211:225	hydroxyl groups (h-BN-OH)	211:235	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	1	55	theme	hydroxyl	211:218	arg1	h-BN-OH					228:234	h-BN-OH	228:234	h-BN-OH	228:234	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	9	56	theme	paper	1587:1591	arg1	samples					1593:1599	the control paper samples	1575:1599	the control paper samples	1575:1599	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	57	theme	samples	1473:1479	arg1	strength					1451:1458	bursting strength	1442:1458	bursting strength	1442:1458	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	57	theme	samples	1473:1479	arg1	strength					1410:1417	Tensile strength	1402:1417	Tensile strength	1402:1417	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	9	57	theme	samples	1473:1479	arg1	strength					1428:1435	tearing strength	1420:1435	tearing strength	1420:1435	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	10	58	theme	release	1708:1714	arg1	rate					1716:1719	the composite paper-the heat release rate	1679:1719	the composite paper-the heat release rate decreased by up to 11.6%	1679:1744	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	9	59	theme	tearing	1420:1426	arg1	strength					1428:1435	tearing strength	1420:1435	tearing strength	1420:1435	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	5	60	theme	Gram-positive	994:1006	arg1	bacteria					1031:1038	both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria	961:1038	bacteria	1031:1038	All the samples were tested for the antibacterial properties via the colony forming unit method and exhibited good performance against both Gram-negative (E. coli) and Gram-positive (S. epidermidis) model bacteria.					
32751335	10	61	theme	paper-the	1693:1701	arg1	rate					1716:1719	the composite paper-the heat release rate	1679:1719	the composite paper-the heat release rate decreased by up to 11.6%	1679:1744	Furthermore, the nanofiller improved the thermal properties of the composite paper-the heat release rate decreased by up to 11.6%.					
32751335	4	62	theme	wt	819:820	arg1	%					822:822	3 wt %	817:822	3 wt %	817:822	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	6	63	theme	antibacterial	1122:1134	arg1	efficiency					1136:1145	the antibacterial efficiency	1118:1145	the antibacterial efficiency of the obtained materials	1118:1171	Additionally, the influence of the volume of working bacterial suspension on the antibacterial efficiency of the obtained materials was examined.					
32751335	9	64	theme	bursting	1442:1449	arg1	strength					1451:1458	bursting strength	1442:1458	bursting strength	1442:1458	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	4	65	theme	cellulose	687:695	arg1	fibers					697:702	Modified cellulose fibers	678:702	Modified cellulose fibers	678:702	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	9	66	dep	samples	1593:1599	arg1	comparison					1561:1570	comparison	1561:1570	comparison	1561:1570	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	7	67	theme	suspension	1282:1291	arg1	volume					1262:1267	the volume	1258:1267	the volume of bacterial suspension	1258:1291	The results showed significantly better antibacterial performance when the volume of bacterial suspension was reduced.					
32751335	0	68	theme	Antibacterial	79:91	arg1	Response					93:100	Superior Antibacterial Response	70:100	Superior Antibacterial Response	70:100	Few Layered Oxidized h-BN as Nanofiller of Cellulose-Based Paper with Superior Antibacterial Response and Enhanced Mechanical/Thermal Performance.					
32751335	1	69	theme	hexagonal	162:170	arg1	nanosheets					186:195	hexagonal boron nitride nanosheets	162:195	hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH)	162:235	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	1	70	theme	bulk	358:361	arg1	h-BN					363:366	bulk h-BN	358:366	bulk h-BN	358:366	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	4	71	theme	paper	725:729	arg1	samples					738:744	paper sheets samples	725:744	paper sheets samples	725:744	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	0	72	theme	Mechanical/Thermal	115:132	arg1	Performance					134:144	Enhanced Mechanical/Thermal Performance	106:144	Enhanced Mechanical/Thermal Performance	106:144	Few Layered Oxidized h-BN as Nanofiller of Cellulose-Based Paper with Superior Antibacterial Response and Enhanced Mechanical/Thermal Performance.					
32751335	3	73	dep	spectroscopic	486:498	arg1	XRD					508:510	XRD	508:510	XRD	508:510	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	3	73	dep	spectroscopic	486:498	arg1	FT-IR					501:505	FT-IR	501:505	FT-IR	501:505	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32751335	2	74	theme	cellulose	431:439	arg1	fibers					441:446	cellulose fibers	431:446	cellulose fibers	431:446	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	1	75	theme	fibers	291:296	arg1	surface					270:276	the surface	266:276	the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN	266:366	In this study, hexagonal boron nitride nanosheets enriched with hydroxyl groups (h-BN-OH) were successfully grafted on the surface of cellulose fibers after the simple and effective exfoliation and oxidation of bulk h-BN.					
32751335	11	76	theme	antibacterial	1821:1833	arg1	coatings					1873:1880	paper coatings	1867:1880	paper coatings	1867:1880	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	11	76	theme	antibacterial	1821:1833	arg1	materials					1835:1843	antibacterial materials	1821:1843	antibacterial materials	1821:1843	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	11	76	theme	antibacterial	1821:1833	arg1	packaging					1854:1862	packaging	1854:1862	packaging	1854:1862	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	7	77	theme	antibacterial	1227:1239	arg1	performance					1241:1251	significantly better antibacterial performance	1206:1251	significantly better antibacterial performance	1206:1251	The results showed significantly better antibacterial performance when the volume of bacterial suspension was reduced.					
32751335	9	78	theme	2	1497:1497	arg1	wt					1499:1500	wt	1499:1500	wt	1499:1500	Tensile strength, tearing strength, and bursting strength of the paper samples containing only 2 wt % of the nanofiller were improved by 60%, 61%, and 118% in comparison to the control paper samples, respectively.					
32751335	11	79	theme	wide	1807:1810	arg1	coatings					1873:1880	paper coatings	1867:1880	paper coatings	1867:1880	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	11	79	theme	wide	1807:1810	arg1	range					1812:1816	a wide range	1805:1816	a wide range of antibacterial materials, such as packaging or paper coatings	1805:1880	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	11	79	theme	wide	1807:1810	arg1	packaging					1854:1862	packaging	1854:1862	packaging	1854:1862	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	2	80	theme	hydrogen	463:470	arg1	bonding					472:478	hydrogen bonding	463:478	hydrogen bonding	463:478	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	4	81	theme	nanomaterial	783:794	arg1	concentrations					761:774	different concentrations	751:774	different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %)	751:823	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	0	82	theme	Cellulose-Based	43:57	arg1	Paper					59:63	Cellulose-Based Paper	43:63	Cellulose-Based Paper	43:63	Few Layered Oxidized h-BN as Nanofiller of Cellulose-Based Paper with Superior Antibacterial Response and Enhanced Mechanical/Thermal Performance.					
32751335	8	83	theme	samples	1341:1347	arg1	properties					1317:1326	Mechanical properties	1306:1326	Mechanical properties of the paper samples with and without nanofiller	1306:1375	Mechanical properties of the paper samples with and without nanofiller were also characterized.					
32751335	11	84	theme	materials	1835:1843	arg1	coatings					1873:1880	paper coatings	1867:1880	paper coatings	1867:1880	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	11	84	theme	materials	1835:1843	arg1	range					1812:1816	a wide range	1805:1816	a wide range of antibacterial materials, such as packaging or paper coatings	1805:1880	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	11	84	theme	materials	1835:1843	arg1	packaging					1854:1862	packaging	1854:1862	packaging	1854:1862	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	4	85	theme	different	751:759	arg1	concentrations					761:774	different concentrations	751:774	different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %)	751:823	Modified cellulose fibers were used to prepare paper sheets samples with different concentrations of the nanomaterial (1 wt %, 2 wt %, and 3 wt %).					
32751335	11	86	theme	composite	1762:1770	arg1	paper					1772:1776	the composite paper	1758:1776	the composite paper	1758:1776	Therefore, the composite paper can be further explored in a wide range of antibacterial materials, such as packaging or paper coatings.					
32751335	2	87	theme	OH	369:370	arg1	h-BN-OH					382:388	h-BN-OH	382:388	h-BN-OH	382:388	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	2	87	theme	OH	369:370	arg1	groups					372:377	OH groups	369:377	OH groups of h-BN-OH	369:388	OH groups of h-BN-OH and the ones presented on the surface of cellulose fibers interacted via hydrogen bonding.					
32751335	3	88	with	fibers	648:653	arg1	nanomaterial					664:675	the nanomaterial	660:675	the nanomaterial	660:675	Both spectroscopic (FT-IR, XRD) and microscopic (TEM, SEM, and atomic force microscopy (AFM)) methods results proved the successful functionalization of the cellulose fibers with the nanomaterial.					
32253406	11	0	theme	MUC2	1653:1656	arg1	genes					1704:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	12	1	theme	enhanced	1769:1776	arg1	integrity					1784:1792	enhanced mucus integrity	1769:1792	enhanced mucus integrity	1769:1792	We identify structural features of GOS that contribute to enhanced mucus integrity.					
32253406	9	2	from	modulation	1419:1428	arg1	preparations					1445:1456	(β1 → 4)GOS preparations	1433:1456	(β1 → 4)GOS preparations	1433:1456	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	1	3	theme	goblet	260:265	arg1	cells					267:271	intestinal goblet cells	249:271	intestinal goblet cells	249:271	Galacto-oligosaccharides (GOS) have been reported to modulate the function of intestinal goblet cells and to improve mucus barrier function.					
32253406	3	4	theme	oligosaccharide	516:530	arg1	content					532:538	oligosaccharide content	516:538	oligosaccharide content	516:538	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	12	5	theme	GOS	1746:1748	arg1	features					1734:1741	structural features	1723:1741	structural features of GOS that contribute to enhanced mucus integrity	1723:1792	We identify structural features of GOS that contribute to enhanced mucus integrity.					
32253406	2	6	from	available	328:336	arg1	compositions					369:380	many structurally different compositions	341:380	many structurally different compositions	341:380	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	10	7	theme	β1	1460:1461	arg1	→					1463:1463	β1 →	1460:1463	β1 →	1460:1463	(β1 → 3)GOS induced the expression of MUC2 and TFF3, and downregulated the RETNLB gene.					
32253406	3	8	theme	GOS	567:569	arg1	content					532:538	oligosaccharide content	516:538	oligosaccharide content	516:538	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	3	8	theme	GOS	567:569	arg1	linkages					555:562	glycosidic linkages	544:562	glycosidic linkages of GOS	544:569	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	6	9	theme	genes	1072:1076	arg1	expression					1044:1053	mRNA expression	1039:1053	mRNA expression of mucus-related genes	1039:1076	The changes in mRNA expression of mucus-related genes were assessed by RT-PCR.					
32253406	11	10	theme	β1	1583:1584	arg1	→					1586:1586	β1 → 4	1583:1588	β1 → 4	1583:1588	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	11	10	theme	β1	1583:1584	arg1	GOS					1590:1592	the (β1 → 3) and GOS (β1 → 4)GOS	1561:1592	GOS	1590:1592	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	5	11	theme	oligosaccharides	967:982	arg1	mixture					947:953	a mixture	945:953	a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO)	945:1021	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	3	12	theme	linkages	555:562	arg1	effects					505:511	the effects	501:511	the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells	501:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	11	13	theme	RETNLB	1665:1670	arg1	genes					1704:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	5	14	theme	lactose-derived	985:999	arg1	oligosaccharides					967:982	α-linked oligosaccharides	958:982	α-linked oligosaccharides (lactose-derived oligosaccharides-LDO)	958:1021	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	14	theme	lactose-derived	985:999	arg1	oligosaccharides-LDO					1001:1020	lactose-derived oligosaccharides-LDO	985:1020	lactose-derived oligosaccharides-LDO	985:1020	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	3	15	theme	genes	588:592	arg1	expression					574:583	expression	574:583	expression of genes associated with the secretory function of goblet cells	574:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	4	16	theme	LS174T	704:709	arg1	cells					711:715	LS174T cells	704:715	LS174T cells	704:715	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	0	17	theme	mucus-related	134:146	arg1	genes					148:152	mucus-related genes	134:152	mucus-related genes	134:152	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	11	18	link	α-linked	1599:1606	arg1	LDO					1608:1610	the α-linked LDO	1595:1610	the α-linked LDO	1595:1610	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	3	19	theme	secretory	614:622	arg1	function					624:631	the secretory function	610:631	the secretory function of goblet cells	610:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	7	20	theme	MUC2	1163:1166	arg1	expression					1149:1158	the expression	1145:1158	the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes	1145:1224	GOS containing Lac significantly enhanced the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes.					
32253406	9	21	theme	goblet	1407:1412	arg1	modulation					1419:1428	goblet cell modulation	1407:1428	goblet cell modulation in (β1 → 4)GOS preparations	1407:1456	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	5	22	link	lactose-derived	985:999	arg1	oligosaccharides					967:982	α-linked oligosaccharides	958:982	α-linked oligosaccharides (lactose-derived oligosaccharides-LDO)	958:1021	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	22	link	lactose-derived	985:999	arg1	oligosaccharides-LDO					1001:1020	lactose-derived oligosaccharides-LDO	985:1020	lactose-derived oligosaccharides-LDO	985:1020	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	4	23	theme	content	695:701	arg1	effect					669:674	the effect	665:674	the effect of oligosaccharide content	665:701	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	11	24	theme	GOS	1578:1580	arg1	→					1586:1586	β1 → 4	1583:1588	β1 → 4	1583:1588	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	11	24	theme	GOS	1578:1580	arg1	GOS					1590:1592	the (β1 → 3) and GOS (β1 → 4)GOS	1561:1592	GOS	1590:1592	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	0	25	theme	content	71:77	arg1	impact					4:9	The impact	0:9	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.	0:169	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	0	26	theme	galacto-oligosaccharides	82:105	arg1	content					71:77	lactose content	63:77	lactose content of galacto-oligosaccharides (GOS)	63:111	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	0	26	theme	galacto-oligosaccharides	82:105	arg1	GOS					108:110	GOS	108:110	GOS	108:110	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	0	26	theme	galacto-oligosaccharides	82:105	arg1	content					30:36	oligosaccharide content	14:36	oligosaccharide content	14:36	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	0	26	theme	galacto-oligosaccharides	82:105	arg1	linkages					50:57	glycosidic linkages	39:57	glycosidic linkages	39:57	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	5	27	theme	GOS	914:916	arg1	effects					895:901	the effects	891:901	the effects of (β1 → 4)GOS	891:916	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	10	28	theme	MUC2	1497:1500	arg1	expression					1483:1492	the expression	1479:1492	the expression of MUC2 and TFF3	1479:1509	(β1 → 3)GOS induced the expression of MUC2 and TFF3, and downregulated the RETNLB gene.					
32253406	13	29	theme	better	1819:1824	arg1	formulations					1830:1841	better GOS formulations	1819:1841	better GOS formulations for foods	1819:1851	Our study might lead to better GOS formulations for foods to prevent or treat different types of intestinal disorders.					
32253406	1	30	theme	cells	267:271	arg1	function					237:244	the function	233:244	the function of intestinal goblet cells	233:271	Galacto-oligosaccharides (GOS) have been reported to modulate the function of intestinal goblet cells and to improve mucus barrier function.					
32253406	13	31	theme	different	1873:1881	arg1	types					1883:1887	different types	1873:1887	different types of intestinal disorders	1873:1911	Our study might lead to better GOS formulations for foods to prevent or treat different types of intestinal disorders.					
32253406	5	32	theme	β1	907:908	arg1	→					910:910	β1 → 4	907:912	β1 → 4	907:912	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	32	theme	β1	907:908	arg1	GOS					914:916	(β1 → 4)GOS	906:916	(β1 → 4)GOS	906:916	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	3	33	theme	cells	643:647	arg1	function					624:631	the secretory function	610:631	the secretory function of goblet cells	610:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	2	34	theme	GOS	404:406	arg1	diversity					419:427	GOS structural diversity	404:427	GOS structural diversity	404:427	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	2	35	theme	cells	463:467	arg1	modulation					442:451	this modulation	437:451	this modulation of goblet cells	437:467	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	11	36	theme	Golgi	1680:1684	arg1	sulfotransferases					1686:1702	the Golgi sulfotransferases	1676:1702	the Golgi sulfotransferases	1676:1702	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	4	37	theme	lactose	802:808	arg1	composition					816:826	lactose (Lac) composition	802:826	lactose (Lac) composition	802:826	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	10	38	theme	TFF3	1506:1509	arg1	expression					1483:1492	the expression	1479:1492	the expression of MUC2 and TFF3	1479:1509	(β1 → 3)GOS induced the expression of MUC2 and TFF3, and downregulated the RETNLB gene.					
32253406	0	39	theme	content	30:36	arg1	impact					4:9	The impact	0:9	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.	0:169	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	1	40	theme	mucus	288:292	arg1	function					302:309	mucus barrier function	288:309	mucus barrier function	288:309	Galacto-oligosaccharides (GOS) have been reported to modulate the function of intestinal goblet cells and to improve mucus barrier function.					
32253406	2	41	from	compositions	369:380	arg1	available					328:336	available	328:336	available	328:336	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	7	42	theme	genes	1220:1224	arg1	expression					1149:1158	the expression	1145:1158	the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes	1145:1224	GOS containing Lac significantly enhanced the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes.					
32253406	4	43	theme	Lac	811:813	arg1	composition					816:826	lactose (Lac) composition	802:826	lactose (Lac) composition	802:826	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	7	44	contain	containing	1107:1116	arg2	Lac					1118:1120	Lac	1118:1120	Lac	1118:1120	GOS containing Lac significantly enhanced the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes.					
32253406	7	44	contain	containing	1107:1116	arg1	GOS					1103:1105	GOS	1103:1105	GOS containing Lac	1103:1120	GOS containing Lac significantly enhanced the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes.					
32253406	0	45	theme	glycosidic	39:48	arg1	linkages					50:57	glycosidic linkages	39:57	glycosidic linkages	39:57	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	7	46	theme	Golgi	1196:1200	arg1	genes					1220:1224	Golgi sulfotransferases genes	1196:1224	Golgi sulfotransferases genes	1196:1224	GOS containing Lac significantly enhanced the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes.					
32253406	4	47	theme	variable	752:759	arg1	oligosaccharides					781:796	variable transgalactosylated oligosaccharides	752:796	variable transgalactosylated oligosaccharides	752:796	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	5	48	theme	linkages	869:876	arg1	effect					848:853	the effect	844:853	the effect of glycosidic linkages	844:876	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	0	49	from	expression	120:129	arg1	cells					164:168	goblet cells	157:168	goblet cells	157:168	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	4	50	theme	oligosaccharides	781:796	arg1	composition					816:826	lactose (Lac) composition	802:826	lactose (Lac) composition	802:826	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	4	50	theme	oligosaccharides	781:796	arg1	→					741:741	β1 → 4	738:743	β1 → 4	738:743	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	4	50	theme	oligosaccharides	781:796	arg1	GOS					745:747	GOS	745:747	GOS of variable transgalactosylated oligosaccharides	745:796	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	11	51	theme	expression	1642:1651	arg1	MUC2					1653:1656	expression MUC2	1642:1656	expression MUC2	1642:1656	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	10	52	dep	induced	1471:1477	arg1	3					1465:1465	3	1465:1465	3	1465:1465	(β1 → 3)GOS induced the expression of MUC2 and TFF3, and downregulated the RETNLB gene.					
32253406	3	53	theme	content	532:538	arg1	effects					505:511	the effects	501:511	the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells	501:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	1	54	theme	intestinal	249:258	arg1	cells					267:271	intestinal goblet cells	249:271	intestinal goblet cells	249:271	Galacto-oligosaccharides (GOS) have been reported to modulate the function of intestinal goblet cells and to improve mucus barrier function.					
32253406	13	55	theme	intestinal	1892:1901	arg1	disorders					1903:1911	intestinal disorders	1892:1911	intestinal disorders	1892:1911	Our study might lead to better GOS formulations for foods to prevent or treat different types of intestinal disorders.					
32253406	3	56	from	effects	505:511	arg1	expression					574:583	expression	574:583	expression of genes associated with the secretory function of goblet cells	574:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	12	57	theme	mucus	1778:1782	arg1	integrity					1784:1792	enhanced mucus integrity	1769:1792	enhanced mucus integrity	1769:1792	We identify structural features of GOS that contribute to enhanced mucus integrity.					
32253406	3	58	theme	glycosidic	544:553	arg1	linkages					555:562	glycosidic linkages	544:562	glycosidic linkages of GOS	544:569	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	9	59	theme	GAL3ST2	1348:1354	arg1	genes					1356:1360	GAL3ST2 genes	1348:1360	GAL3ST2 genes	1348:1360	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	6	60	theme	mucus-related	1058:1070	arg1	genes					1072:1076	mucus-related genes	1058:1076	mucus-related genes	1058:1076	The changes in mRNA expression of mucus-related genes were assessed by RT-PCR.					
32253406	11	61	theme	α-linked	1599:1606	arg1	LDO					1608:1610	the α-linked LDO	1595:1610	the α-linked LDO	1595:1610	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	0	62	theme	genes	148:152	arg1	expression					120:129	the expression	116:129	the expression of mucus-related genes in goblet cells	116:168	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	13	63	theme	disorders	1903:1911	arg1	types					1883:1887	different types	1873:1887	different types of intestinal disorders	1873:1911	Our study might lead to better GOS formulations for foods to prevent or treat different types of intestinal disorders.					
32253406	12	64	theme	structural	1723:1732	arg1	features					1734:1741	structural features	1723:1741	structural features of GOS that contribute to enhanced mucus integrity	1723:1792	We identify structural features of GOS that contribute to enhanced mucus integrity.					
32253406	0	65	theme	goblet	157:162	arg1	cells					164:168	goblet cells	157:168	goblet cells	157:168	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	5	66	link	α-linked	958:965	arg1	oligosaccharides					967:982	α-linked oligosaccharides	958:982	α-linked oligosaccharides (lactose-derived oligosaccharides-LDO)	958:1021	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	66	link	α-linked	958:965	arg1	oligosaccharides-LDO					1001:1020	lactose-derived oligosaccharides-LDO	985:1020	lactose-derived oligosaccharides-LDO	985:1020	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	0	67	theme	lactose	63:69	arg1	content					71:77	lactose content	63:77	lactose content of galacto-oligosaccharides (GOS)	63:111	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	0	67	theme	lactose	63:69	arg1	GOS					108:110	GOS	108:110	GOS	108:110	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	5	68	theme	α-linked	958:965	arg1	oligosaccharides					967:982	α-linked oligosaccharides	958:982	α-linked oligosaccharides (lactose-derived oligosaccharides-LDO)	958:1021	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	68	theme	α-linked	958:965	arg1	oligosaccharides-LDO					1001:1020	lactose-derived oligosaccharides-LDO	985:1020	lactose-derived oligosaccharides-LDO	985:1020	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	69	theme	glycosidic	858:867	arg1	linkages					869:876	glycosidic linkages	858:876	glycosidic linkages	858:876	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	11	70	theme	β1	1566:1567	arg1	→					1569:1569	β1 → 3	1566:1571	β1 → 3	1566:1571	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	9	71	theme	cell	1414:1417	arg1	modulation					1419:1428	goblet cell modulation	1407:1428	goblet cell modulation in (β1 → 4)GOS preparations	1407:1456	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	0	72	from	impact	4:9	arg1	expression					120:129	the expression	116:129	the expression of mucus-related genes in goblet cells	116:168	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	4	73	theme	oligosaccharide	679:693	arg1	content					695:701	oligosaccharide content	679:701	oligosaccharide content	679:701	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	9	74	theme	β1	1434:1435	arg1	preparations					1445:1456	(β1 → 4)GOS preparations	1433:1456	(β1 → 4)GOS preparations	1433:1456	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	13	75	theme	GOS	1826:1828	arg1	formulations					1830:1841	better GOS formulations	1819:1841	better GOS formulations for foods	1819:1851	Our study might lead to better GOS formulations for foods to prevent or treat different types of intestinal disorders.					
32253406	9	76	theme	4	1439:1439	arg1	preparations					1445:1456	(β1 → 4)GOS preparations	1433:1456	(β1 → 4)GOS preparations	1433:1456	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	2	77	theme	different	359:367	arg1	compositions					369:380	many structurally different compositions	341:380	many structurally different compositions	341:380	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	0	78	theme	oligosaccharide	14:28	arg1	content					30:36	oligosaccharide content	14:36	oligosaccharide content	14:36	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	9	79	theme	GOS	1441:1443	arg1	preparations					1445:1456	(β1 → 4)GOS preparations	1433:1456	(β1 → 4)GOS preparations	1433:1456	Lac alone significantly enhanced MUC2, TFF3, RETNLB, CHST5, and GAL3ST2 genes suggesting that Lac might be responsible for goblet cell modulation in (β1 → 4)GOS preparations.					
32253406	2	80	theme	structural	408:417	arg1	diversity					419:427	GOS structural diversity	404:427	GOS structural diversity	404:427	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	3	81	theme	goblet	636:641	arg1	cells					643:647	goblet cells	636:647	goblet cells	636:647	This study aims to investigate the effects of oligosaccharide content and glycosidic linkages of GOS on expression of genes associated with the secretory function of goblet cells.					
32253406	7	82	theme	sulfotransferases	1202:1218	arg1	genes					1220:1224	Golgi sulfotransferases genes	1196:1224	Golgi sulfotransferases genes	1196:1224	GOS containing Lac significantly enhanced the expression of MUC2, TFF3 and RETNLB but not of Golgi sulfotransferases genes.					
32253406	2	83	theme	goblet	456:461	arg1	cells					463:467	goblet cells	456:467	goblet cells	456:467	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	11	84	theme	sulfotransferases	1686:1702	arg1	genes					1704:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
32253406	0	85	theme	linkages	50:57	arg1	impact					4:9	The impact	0:9	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.	0:169	The impact of oligosaccharide content, glycosidic linkages and lactose content of galacto-oligosaccharides (GOS) on the expression of mucus-related genes in goblet cells.					
32253406	1	86	theme	barrier	294:300	arg1	function					302:309	mucus barrier function	288:309	mucus barrier function	288:309	Galacto-oligosaccharides (GOS) have been reported to modulate the function of intestinal goblet cells and to improve mucus barrier function.					
32253406	10	87	theme	RETNLB	1534:1539	arg1	gene					1541:1544	the RETNLB gene	1530:1544	the RETNLB gene	1530:1544	(β1 → 3)GOS induced the expression of MUC2 and TFF3, and downregulated the RETNLB gene.					
32253406	8	88	from	impact	1264:1269	arg1	contrast					1230:1237	contrast	1230:1237	contrast	1230:1237	In contrast, GOS without Lac did not impact these genes.					
32253406	5	89	theme	β1	924:925	arg1	→					927:927	β1 → 3	924:929	β1 → 3	924:929	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	5	89	theme	β1	924:925	arg1	GOS					931:933	(β1 → 3)GOS	923:933	(β1 → 3)GOS	923:933	To investigate the effect of glycosidic linkages, we compared the effects of (β1 → 4)GOS with (β1 → 3)GOS, and with a mixture of α-linked oligosaccharides (lactose-derived oligosaccharides-LDO).					
32253406	6	90	theme	mRNA	1039:1042	arg1	expression					1044:1053	mRNA expression	1039:1053	mRNA expression of mucus-related genes	1039:1076	The changes in mRNA expression of mucus-related genes were assessed by RT-PCR.					
32253406	2	91	theme	many	341:344	arg1	compositions					369:380	many structurally different compositions	341:380	many structurally different compositions	341:380	However, GOS is available in many structurally different compositions and it is unknown how GOS structural diversity impacts this modulation of goblet cells.					
32253406	4	92	theme	transgalactosylated	761:779	arg1	oligosaccharides					781:796	variable transgalactosylated oligosaccharides	752:796	variable transgalactosylated oligosaccharides	752:796	To investigate the effect of oligosaccharide content, LS174T cells were incubated with (β1 → 4)GOS of variable transgalactosylated oligosaccharides and lactose (Lac) composition.					
32253406	6	93	from	changes	1028:1034	arg1	expression					1044:1053	mRNA expression	1039:1053	mRNA expression of mucus-related genes	1039:1076	The changes in mRNA expression of mucus-related genes were assessed by RT-PCR.					
32253406	11	94	theme	TFF3	1659:1662	arg1	genes					1704:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes	1638:1708	Compared with the (β1 → 3) and GOS (β1 → 4)GOS, the α-linked LDO significantly upregulated the expression MUC2, TFF3, RETNLB and the Golgi sulfotransferases genes.					
33232701	7	0	theme	CuO	1071:1073	arg1	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	0	theme	CuO	1071:1073	arg1	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	1	1	theme	aqueous	281:287	arg1	environment					289:299	the aqueous environment	277:299	the aqueous environment	277:299	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	2	theme	novel	139:143	arg1	nanocomposite					177:189	A novel, sustainable chitosan polymeric nanocomposite	137:189	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO)	137:202	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	2	theme	novel	139:143	arg1	CuO					199:201	CS-PVA@CuO	192:201	CS-PVA@CuO	192:201	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	7	3	theme	polymer	1075:1081	arg1	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	3	theme	polymer	1075:1081	arg1	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	4	theme	CS-PVA	1012:1017	arg1	CuO					1019:1021	the CS-PVA@CuO	1008:1021	the CS-PVA@CuO was 171.4 mg/g at 313 K.	1008:1046	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	6	5	from	spontaneous	903:913	arg1	nature					934:939	nature	934:939	nature	934:939	Thermodynamics study typically revealed that the uptake of AB25 by the adsorbent is spontaneous and endothermic in nature.					
33232701	2	6	theme	adsorption	460:469	arg1	process					471:477	the batch adsorption process	450:477	the batch adsorption process	450:477	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	7	7	theme	AB25	1195:1198	arg1	dye					1200:1202	anionic AB25 dye	1187:1202	anionic AB25 dye	1187:1202	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	2	8	theme	adsorption	409:418	arg1	kinetics					420:427	adsorption kinetics	409:427	adsorption kinetics	409:427	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	4	9	theme	kinetics	661:668	arg1	equations					670:678	intra-particle diffusion kinetics equations	636:678	intra-particle diffusion kinetics equations	636:678	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	6	10	theme	AB25	878:881	arg1	spontaneous					903:913	spontaneous	903:913	spontaneous	903:913	Thermodynamics study typically revealed that the uptake of AB25 by the adsorbent is spontaneous and endothermic in nature.					
33232701	6	10	theme	AB25	878:881	arg1	uptake					868:873	the uptake	864:873	the uptake of AB25 by the adsorbent	864:898	Thermodynamics study typically revealed that the uptake of AB25 by the adsorbent is spontaneous and endothermic in nature.					
33232701	2	11	theme	batch	454:458	arg1	process					471:477	the batch adsorption process	450:477	the batch adsorption process	450:477	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	4	12	theme	diffusion	651:659	arg1	equations					670:678	intra-particle diffusion kinetics equations	636:678	intra-particle diffusion kinetics equations	636:678	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	3	13	theme	CS-PVA	484:489	arg1	composite					495:503	The CS-PVA@CuO composite	480:503	The CS-PVA@CuO composite	480:503	The CS-PVA@CuO composite was systematically characterized by XRD, FTIR, SEM, and EDX analysis.					
33232701	0	14	theme	Blue	103:106	arg1	Acid					98:101	Acid Blue 25	98:109	Acid Blue 25	98:109	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	7	15	from	313 K.	1041:1046	arg1	171.4 mg/g					1027:1036	171.4 mg/g	1027:1036	171.4 mg/g	1027:1036	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	4	16	theme	process	736:742	arg1	data					713:716	the kinetic data	701:716	the kinetic data of the adsorption process	701:742	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	5	17	theme	adsorption	749:758	arg1	kinetics					760:767	The adsorption kinetics	745:767	The adsorption kinetics	745:767	The adsorption kinetics confirms that the PSO model was a more exact fit.					
33232701	0	18	theme	Acid	98:101	arg1	removal					87:93	the removal	83:93	the removal of Acid Blue 25 from aqueous environment	83:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	1	19	from	environment	289:299	arg1	acid					252:255	acid blue 25 (AB25)	252:270	acid blue 25 (AB25) from the aqueous environment	252:299	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	19	from	environment	289:299	arg1	removal					241:247	the removal	237:247	the removal of acid blue 25 (AB25) from the aqueous environment	237:299	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	20	theme	chitosan	158:165	arg1	nanocomposite					177:189	A novel, sustainable chitosan polymeric nanocomposite	137:189	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO)	137:202	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	20	theme	chitosan	158:165	arg1	CuO					199:201	CS-PVA@CuO	192:201	CS-PVA@CuO	192:201	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	21	dep	novel	139:143	arg1	sustainable					146:156	sustainable	146:156	sustainable	146:156	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	7	22	from	solutions	1221:1229	arg1	elimination					1172:1182	the potential elimination	1158:1182	the potential elimination of anionic AB25 dye from the aqueous solutions	1158:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	1	23	theme	polymeric	167:175	arg1	nanocomposite					177:189	A novel, sustainable chitosan polymeric nanocomposite	137:189	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO)	137:202	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	23	theme	polymeric	167:175	arg1	CuO					199:201	CS-PVA@CuO	192:201	CS-PVA@CuO	192:201	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	7	24	theme	highest	977:983	arg1	capacity					996:1003	the highest adsorption capacity	973:1003	the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific	973:1061	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	2	25	theme	variables	329:337	arg1	influence					306:314	The influence	302:314	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics	302:427	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	0	26	theme	chitosan	21:28	arg1	nanocomposite					40:52	novel chitosan polymeric nanocomposite	15:52	novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment	15:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	1	27	theme	acid	252:255	arg1	removal					241:247	the removal	237:247	the removal of acid blue 25 (AB25) from the aqueous environment	237:299	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	6	28	from	nature	934:939	arg1	spontaneous					903:913	spontaneous	903:913	spontaneous	903:913	Thermodynamics study typically revealed that the uptake of AB25 by the adsorbent is spontaneous and endothermic in nature.					
33232701	6	28	from	nature	934:939	arg1	uptake					868:873	the uptake	864:873	the uptake of AB25 by the adsorbent	864:898	Thermodynamics study typically revealed that the uptake of AB25 by the adsorbent is spontaneous and endothermic in nature.					
33232701	3	29	theme	@	490:490	arg1	composite					495:503	The CS-PVA@CuO composite	480:503	The CS-PVA@CuO composite	480:503	The CS-PVA@CuO composite was systematically characterized by XRD, FTIR, SEM, and EDX analysis.					
33232701	7	30	theme	adsorption	985:994	arg1	capacity					996:1003	the highest adsorption capacity	973:1003	the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific	973:1061	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	31	theme	potential	1162:1170	arg1	elimination					1172:1182	the potential elimination	1158:1182	the potential elimination of anionic AB25 dye from the aqueous solutions	1158:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	0	32	theme	novel	15:19	arg1	nanocomposite					40:52	novel chitosan polymeric nanocomposite	15:52	novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment	15:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	1	33	theme	blue	257:260	arg1	acid					252:255	acid blue 25 (AB25)	252:270	acid blue 25 (AB25) from the aqueous environment	252:299	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	34	from	removal	241:247	arg1	environment					289:299	the aqueous environment	277:299	the aqueous environment	277:299	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	3	35	theme	EDX	561:563	arg1	analysis					565:572	EDX analysis	561:572	EDX analysis	561:572	The CS-PVA@CuO composite was systematically characterized by XRD, FTIR, SEM, and EDX analysis.					
33232701	3	36	theme	CuO	491:493	arg1	composite					495:503	The CS-PVA@CuO composite	480:503	The CS-PVA@CuO composite	480:503	The CS-PVA@CuO composite was systematically characterized by XRD, FTIR, SEM, and EDX analysis.					
33232701	4	37	used	used	685:688	arg2	order					592:596	The pseudo-first order	575:596	The pseudo-first order (PFO)	575:602	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	37	used	used	685:688	arg2	PSO					626:628	PSO	626:628	PSO	626:628	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	37	used	used	685:688	arg2	order					619:623	pseudo-second order	605:623	pseudo-second order (PSO)	605:629	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	37	used	used	685:688	arg2	equations					670:678	intra-particle diffusion kinetics equations	636:678	intra-particle diffusion kinetics equations	636:678	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	37	used	used	685:688	arg2	PFO					599:601	PFO	599:601	PFO	599:601	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	1	38	theme	CS-PVA	192:197	arg1	nanocomposite					177:189	A novel, sustainable chitosan polymeric nanocomposite	137:189	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO)	137:202	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	38	theme	CS-PVA	192:197	arg1	CuO					199:201	CS-PVA@CuO	192:201	CS-PVA@CuO	192:201	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	0	39	theme	nanocomposite	40:52	arg1	Preparation					0:10	Preparation	0:10	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.	0:135	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	2	40	theme	contact	351:357	arg1	time					359:362	contact time	351:362	contact time	351:362	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	4	41	theme	pseudo-first	579:590	arg1	PFO					599:601	PFO	599:601	PFO	599:601	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	41	theme	pseudo-first	579:590	arg1	order					592:596	The pseudo-first order	575:596	The pseudo-first order (PFO)	575:602	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	1	42	theme	@	198:198	arg1	nanocomposite					177:189	A novel, sustainable chitosan polymeric nanocomposite	137:189	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO)	137:202	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	1	42	theme	@	198:198	arg1	CuO					199:201	CS-PVA@CuO	192:201	CS-PVA@CuO	192:201	A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment.					
33232701	0	43	theme	polymeric	30:38	arg1	nanocomposite					40:52	novel chitosan polymeric nanocomposite	15:52	novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment	15:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	0	44	theme	aqueous	116:122	arg1	environment					124:134	aqueous environment	116:134	aqueous environment	116:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	2	45	theme	dye	373:375	arg1	concentration					377:389	initial dye concentration	365:389	initial dye concentration	365:389	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	7	46	theme	@	1018:1018	arg1	CuO					1019:1021	the CS-PVA@CuO	1008:1021	the CS-PVA@CuO was 171.4 mg/g at 313 K.	1008:1046	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	47	theme	CS-PVA	1064:1069	arg1	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	47	theme	CS-PVA	1064:1069	arg1	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	48	theme	adsorbent	1135:1143	arg1	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	48	theme	adsorbent	1135:1143	arg1	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	49	theme	CuO	1019:1021	arg1	capacity					996:1003	the highest adsorption capacity	973:1003	the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific	973:1061	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	6	50	theme	Thermodynamics	819:832	arg1	study					834:838	Thermodynamics study	819:838	Thermodynamics study	819:838	Thermodynamics study typically revealed that the uptake of AB25 by the adsorbent is spontaneous and endothermic in nature.					
33232701	7	51	theme	anionic	1187:1193	arg1	dye					1200:1202	anionic AB25 dye	1187:1202	anionic AB25 dye	1187:1202	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	4	52	theme	adsorption	725:734	arg1	process					736:742	the adsorption process	721:742	the adsorption process	721:742	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	53	theme	pseudo-second	605:617	arg1	order					619:623	pseudo-second order	605:623	pseudo-second order (PSO)	605:629	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	4	53	theme	pseudo-second	605:617	arg1	PSO					626:628	PSO	626:628	PSO	626:628	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	7	54	used	used	1116:1119	arg2	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	54	used	used	1116:1119	arg2	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	55	theme	aqueous	1213:1219	arg1	solutions					1221:1229	the aqueous solutions	1209:1229	the aqueous solutions	1209:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	4	56	theme	kinetic	705:711	arg1	data					713:716	the kinetic data	701:716	the kinetic data of the adsorption process	701:742	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	5	57	theme	exact	808:812	arg1	model					791:795	the PSO model	783:795	the PSO model	783:795	The adsorption kinetics confirms that the PSO model was a more exact fit.					
33232701	5	57	theme	exact	808:812	arg1	fit					814:816	a more exact fit	801:816	a more exact fit	801:816	The adsorption kinetics confirms that the PSO model was a more exact fit.					
33232701	4	58	theme	intra-particle	636:649	arg1	equations					670:678	intra-particle diffusion kinetics equations	636:678	intra-particle diffusion kinetics equations	636:678	The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process.					
33232701	0	59	from	environment	124:134	arg1	removal					87:93	the removal	83:93	the removal of Acid Blue 25 from aqueous environment	83:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	7	60	theme	suitable	1126:1133	arg1	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	60	theme	suitable	1126:1133	arg1	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	61	dep	reveal	966:971	arg1	used					1116:1119	used	1116:1119	can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1097:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	2	62	theme	different	319:327	arg1	pH					347:348	pH	347:348	pH	347:348	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	2	62	theme	different	319:327	arg1	concentration					377:389	initial dye concentration	365:389	initial dye concentration	365:389	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	2	62	theme	different	319:327	arg1	kinetics					420:427	adsorption kinetics	409:427	adsorption kinetics	409:427	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	2	62	theme	different	319:327	arg1	time					359:362	contact time	351:362	contact time	351:362	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	2	62	theme	different	319:327	arg1	temperature					392:402	temperature	392:402	temperature	392:402	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	2	62	theme	different	319:327	arg1	variables					329:337	different variables	319:337	different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics	319:427	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	7	63	theme	dye	1200:1202	arg1	elimination					1172:1182	the potential elimination	1158:1182	the potential elimination of anionic AB25 dye from the aqueous solutions	1158:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	0	64	theme	efficient	60:68	arg1	material					70:77	an efficient material	57:77	an efficient material for the removal of Acid Blue 25 from aqueous environment	57:134	Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.					
33232701	2	65	theme	initial	365:371	arg1	concentration					377:389	initial dye concentration	365:389	initial dye concentration	365:389	The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process.					
33232701	5	66	theme	PSO	787:789	arg1	model					791:795	the PSO model	783:795	the PSO model	783:795	The adsorption kinetics confirms that the PSO model was a more exact fit.					
33232701	5	66	theme	PSO	787:789	arg1	fit					814:816	a more exact fit	801:816	a more exact fit	801:816	The adsorption kinetics confirms that the PSO model was a more exact fit.					
33232701	7	67	theme	@	1070:1070	arg1	nanocomposite					1083:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	CS-PVA@CuO polymer nanocomposite	1064:1095	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
33232701	7	67	theme	@	1070:1070	arg1	material					1145:1152	a suitable adsorbent material	1124:1152	a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions	1124:1229	Remarkably, the results reveal the highest adsorption capacity of the CS-PVA@CuO was 171.4 mg/g at 313 K. To be specific, CS-PVA@CuO polymer nanocomposite can be effectively used as a suitable adsorbent material for the potential elimination of anionic AB25 dye from the aqueous solutions.					
32966939	8	0	theme	acute	1340:1344	arg1	hypoglycemia					1358:1369	acute and chronic hypoglycemia	1340:1369	acute and chronic hypoglycemia	1340:1369	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	6	1	theme	variable	1081:1088	arg1	limits					1090:1095	CCD minimum and maximal variable limits	1057:1095	limits	1090:1095	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	8	2	theme	chronic	1350:1356	arg1	hypoglycemia					1358:1369	acute and chronic hypoglycemia	1340:1369	acute and chronic hypoglycemia	1340:1369	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	3	3	theme	recurring	650:658	arg1	hypoglycemia					660:671	acute and/or recurring hypoglycemia	637:671	acute and/or recurring hypoglycemia	637:671	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	9	4	theme	increased	1650:1658	arg1	VMN					1668:1670	increased, caudal VMN	1650:1670	VMN	1668:1670	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	7	5	theme	collision-induced	1285:1301	arg1	dissociation					1303:1314	collision-induced dissociation	1285:1314	collision-induced dissociation	1285:1314	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	3	6	theme	acute	637:641	arg1	hypoglycemia					660:671	acute and/or recurring hypoglycemia	637:671	acute and/or recurring hypoglycemia	637:671	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	4	7	theme	Central	810:816	arg1	CCD					836:838	CCD	836:838	CCD	836:838	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	4	7	theme	Central	810:816	arg1	Design					828:833	Central Composite Design	810:833	Central Composite Design (CCD)	810:839	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	10	8	theme	tissue	1785:1790	arg1	samples					1792:1798	small-volume brain tissue samples	1766:1798	small-volume brain tissue samples acquired with high-neuroanatomical specificity	1766:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	7	9	theme	source	1274:1279	arg1	optimization					1220:1231	optimization	1220:1231	optimization of solid phase extraction and instrument source and collision-induced dissociation voltages	1220:1323	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	2	10	theme	aromatase	367:375	arg1	expression					377:386	altered aromatase expression	359:386	altered aromatase expression	359:386	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	8	11	theme	E-2	1407:1409	arg1	concentrations					1411:1424	E-2 concentrations	1407:1424	E-2 concentrations relative to baseline	1407:1445	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	6	12	theme	maximal	1140:1146	arg1	response					1148:1155	maximal response	1140:1155	maximal response from CCD	1140:1164	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	5	13	dep	temperature	905:915	arg1	combinations					943:954	variable combinations	934:954	variable combinations	934:954	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	4	14	theme	spectrometric	757:769	arg1	variables					783:791	multiple independent mass spectrometric operational variables	731:791	multiple independent mass spectrometric operational variables	731:791	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	10	15	theme	small-volume	1766:1777	arg1	samples					1792:1798	small-volume brain tissue samples	1766:1798	small-volume brain tissue samples acquired with high-neuroanatomical specificity	1766:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	5	16	theme	Criterion	992:1000	arg1	modeling					1013:1020	Akaike Information Criterion correction modeling	973:1020	Akaike Information Criterion correction modeling	973:1020	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	8	17	theme	relative	1426:1433	arg1	concentrations					1411:1424	E-2 concentrations	1407:1424	E-2 concentrations relative to baseline	1407:1445	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	7	18	dep	source	1274:1279	arg1	voltages					1316:1323	voltages	1316:1323	voltages	1316:1323	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	7	19	theme	phase	1242:1246	arg1	extraction					1248:1257	solid phase extraction	1236:1257	solid phase extraction	1236:1257	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	2	20	theme	reactivity	288:297	arg1	regulation					270:279	E-2 regulation	266:279	E-2 regulation of VMN reactivity to hypoglycemia	266:313	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	6	21	theme	small-run	1108:1116	arg1	model					1118:1122	a small-run model	1106:1122	a small-run model that replicated maximal response from CCD	1106:1164	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	7	22	theme	chromatographic	1171:1185	arg1	response					1187:1194	E-2 chromatographic response	1167:1194	E-2 chromatographic response	1167:1194	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	4	23	theme	E-2	852:854	arg1	area					869:872	E-2 chromatogram area	852:872	E-2 chromatogram area	852:872	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	11	24	theme	E-2	1987:1989	arg1	profiles					1991:1998	E-2 profiles	1987:1998	E-2 profiles	1987:1998	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	5	25	theme	spectrometric	891:903	arg1	temperature					905:915	spectrometric temperature	891:915	spectrometric temperature	891:915	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	5	26	theme	Akaike	973:978	arg1	Criterion					992:1000	Akaike Information Criterion	973:1000	Akaike Information Criterion correction modeling	973:1020	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	0	27	theme	Brain	141:145	arg1	Concentrations					157:170	Brain Estradiol Concentrations	141:170	Brain Estradiol Concentrations	141:170	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	0	28	theme	Concentrations	157:170	arg1	Quantification					123:136	High-Neuroanatomical Resolution Quantification	91:136	High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations	91:170	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	3	29	theme	micropunch	411:420	arg1	tools					433:437	high-resolution micropunch dissection tools	395:437	high-resolution micropunch dissection tools for isolation of segmental VMN tissue	395:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	9	30	dep	VMN	1583:1585	arg1	change					1591:1596	no change	1588:1596	no change	1588:1596	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	6	31	from	CCD	1162:1164	arg1	response					1148:1155	maximal response	1140:1155	maximal response from CCD	1140:1164	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	3	32	theme	Experiments-refined	502:520	arg1	methodology					582:592	Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	502:592	Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	502:592	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	9	33	theme	tissue	1530:1535	arg1	profiles					1541:1548	tissue E-2 profiles	1530:1548	tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1530:1684	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	3	34	theme	segmental	456:464	arg1	tissue					470:475	segmental VMN tissue	456:475	segmental VMN tissue	456:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	0	35	theme	Ultra-High-Performance	0:21	arg1	Spectrometry					74:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry	0:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.	0:171	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	0	36	theme	Chromatography-Electrospray	30:56	arg1	Spectrometry					74:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry	0:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.	0:171	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	9	37	theme	acute	1557:1561	arg1	hypoglycemia					1673:1684	acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1557:1684	acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1557:1684	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	6	38	theme	Sequence	1035:1042	arg1	design					1044:1049	A Fibonacci Sequence design	1023:1049	A Fibonacci Sequence design using CCD minimum and maximal variable limits	1023:1095	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	1	39	theme	hypothalamic	186:197	arg1	VMN					208:210	VMN	208:210	VMN	208:210	Ventromedial hypothalamic nucleus (VMN) control of glucostasis is estradiol (E-2)-dependent.					
32966939	1	39	theme	hypothalamic	186:197	arg1	nucleus					199:205	Ventromedial hypothalamic nucleus	173:205	Ventromedial hypothalamic nucleus (VMN) control of glucostasis	173:234	Ventromedial hypothalamic nucleus (VMN) control of glucostasis is estradiol (E-2)-dependent.					
32966939	2	40	from	changes	327:333	arg1	volume					345:350	signal volume	338:350	signal volume	338:350	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	11	41	theme	VMN	1937:1939	arg1	acclimation					1972:1982	VMN rostro-caudal segment-specific acclimation	1937:1982	VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia	1937:2024	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	3	42	theme	ionization-mass	541:555	arg1	spectrometry					557:568	uHPLC-electrospray ionization-mass spectrometry	522:568	Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	502:592	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	3	42	theme	ionization-mass	541:555	arg1	LC-ESI-MS					571:579	LC-ESI-MS	571:579	LC-ESI-MS	571:579	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	5	43	theme	pressure	925:932	arg1	Combinations					875:886	Combinations	875:886	Combinations of spectrometric temperature and gas pressure variable combinations	875:954	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	9	44	theme	increased	1564:1572	arg1	VMN					1606:1608	caudal VMN	1599:1608	caudal VMN	1599:1608	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	44	theme	increased	1564:1572	arg1	VMN					1583:1585	increased, rostral VMN	1564:1585	VMN	1583:1585	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	10	45	from	sensitivity	1728:1738	arg1	samples					1792:1798	small-volume brain tissue samples	1766:1798	small-volume brain tissue samples acquired with high-neuroanatomical specificity	1766:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	3	46	theme	tissue	470:475	arg1	isolation					443:451	isolation	443:451	isolation of segmental VMN tissue	443:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	9	47	dep	recurring	1615:1623	arg1	change					1629:1634	no change	1626:1634	no change	1626:1634	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	47	dep	recurring	1615:1623	arg1	VMN					1645:1647	rostral VMN	1637:1647	rostral VMN	1637:1647	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	0	48	theme	High-Neuroanatomical	91:110	arg1	Quantification					123:136	High-Neuroanatomical Resolution Quantification	91:136	High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations	91:170	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	9	49	theme	rostral	1575:1581	arg1	VMN					1606:1608	caudal VMN	1599:1608	caudal VMN	1599:1608	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	49	theme	rostral	1575:1581	arg1	VMN					1583:1585	increased, rostral VMN	1564:1585	VMN	1583:1585	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	2	50	theme	E-2	266:268	arg1	regulation					270:279	E-2 regulation	266:279	E-2 regulation of VMN reactivity to hypoglycemia	266:313	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	3	51	used	used	482:485	arg2	tools					433:437	high-resolution micropunch dissection tools	395:437	high-resolution micropunch dissection tools for isolation of segmental VMN tissue	395:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	11	52	theme	segment-specific	1955:1970	arg1	acclimation					1972:1982	VMN rostro-caudal segment-specific acclimation	1937:1982	VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia	1937:2024	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	0	53	theme	Resolution	112:121	arg1	Quantification					123:136	High-Neuroanatomical Resolution Quantification	91:136	High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations	91:170	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	4	54	theme	multiple	731:738	arg1	variables					783:791	multiple independent mass spectrometric operational variables	731:791	multiple independent mass spectrometric operational variables	731:791	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	10	55	theme	requisite	1708:1716	arg1	sensitivity					1728:1738	requisite LC-ESI-MS sensitivity	1708:1738	requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity	1708:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	5	56	theme	temperature	905:915	arg1	Combinations					875:886	Combinations	875:886	Combinations of spectrometric temperature and gas pressure variable combinations	875:954	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	9	57	theme	caudal	1599:1604	arg1	VMN					1606:1608	caudal VMN	1599:1608	caudal VMN	1599:1608	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	57	theme	caudal	1599:1604	arg1	VMN					1583:1585	increased, rostral VMN	1564:1585	VMN	1583:1585	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	6	58	theme	maximal	1073:1079	arg1	limits					1090:1095	CCD minimum and maximal variable limits	1057:1095	limits	1090:1095	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	11	59	theme	Current	1848:1854	arg1	methodology					1856:1866	Current methodology	1848:1866	Current methodology	1848:1866	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	10	60	theme	E-2	1744:1746	arg1	quantification					1748:1761	E-2 quantification	1744:1761	E-2 quantification	1744:1761	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	3	61	from	effects	626:632	arg1	content					684:690	VMN E-2 content	676:690	VMN E-2 content	676:690	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	2	62	theme	signal	338:343	arg1	volume					345:350	signal volume	338:350	signal volume	338:350	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	9	63	theme	recurring	1615:1623	arg1	hypoglycemia					1673:1684	acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1557:1684	acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1557:1684	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	7	64	theme	instrument	1263:1272	arg1	source					1274:1279	instrument source	1263:1279	instrument source	1263:1279	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	3	65	theme	VMN	676:678	arg1	content					684:690	VMN E-2 content	676:690	VMN E-2 content	676:690	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	8	66	theme	rostral	1455:1461	arg1	VMN					1474:1476	both rostral and caudal VMN	1450:1476	both rostral and caudal VMN	1450:1476	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	10	67	theme	brain	1779:1783	arg1	samples					1792:1798	small-volume brain tissue samples	1766:1798	small-volume brain tissue samples acquired with high-neuroanatomical specificity	1766:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	7	68	theme	extraction	1248:1257	arg1	optimization					1220:1231	optimization	1220:1231	optimization of solid phase extraction and instrument source and collision-induced dissociation voltages	1220:1323	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	3	69	theme	hypoglycemia	660:671	arg1	effects					626:632	effects	626:632	effects of acute and/or recurring hypoglycemia on VMN E-2 content	626:690	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	5	70	theme	Information	980:990	arg1	Criterion					992:1000	Akaike Information Criterion	973:1000	Akaike Information Criterion correction modeling	973:1020	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	3	71	theme	E-2	680:682	arg1	content					684:690	VMN E-2 content	676:690	VMN E-2 content	676:690	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	8	72	theme	caudal	1467:1472	arg1	VMN					1474:1476	both rostral and caudal VMN	1450:1476	both rostral and caudal VMN	1450:1476	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	11	73	theme	gluco-regulatory	2048:2063	arg1	function					2065:2072	gluco-regulatory function	2048:2072	gluco-regulatory function	2048:2072	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	7	74	theme	solid	1236:1240	arg1	extraction					1248:1257	solid phase extraction	1236:1257	solid phase extraction	1236:1257	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	5	75	theme	correction	1002:1011	arg1	modeling					1013:1020	Akaike Information Criterion correction modeling	973:1020	Akaike Information Criterion correction modeling	973:1020	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	11	76	from	effects	2037:2043	arg1	function					2065:2072	gluco-regulatory function	2048:2072	gluco-regulatory function	2048:2072	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	9	77	from	variability	1515:1525	arg1	profiles					1541:1548	tissue E-2 profiles	1530:1548	tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1530:1684	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	2	78	theme	VMN	284:286	arg1	reactivity					288:297	VMN reactivity	284:297	VMN reactivity to hypoglycemia	284:313	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	1	79	theme	nucleus	199:205	arg1	control					213:219	Ventromedial hypothalamic nucleus (VMN) control	173:219	Ventromedial hypothalamic nucleus (VMN) control of glucostasis	173:234	Ventromedial hypothalamic nucleus (VMN) control of glucostasis is estradiol (E-2)-dependent.					
32966939	9	80	theme	caudal	1661:1666	arg1	VMN					1668:1670	increased, caudal VMN	1650:1670	VMN	1668:1670	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	2	81	theme	altered	359:365	arg1	expression					377:386	altered aromatase expression	359:386	altered aromatase expression	359:386	E-2 regulation of VMN reactivity to hypoglycemia may involve changes in signal volume due to altered aromatase expression.					
32966939	11	82	theme	recurring	2003:2011	arg1	hypoglycemia					2013:2024	recurring hypoglycemia	2003:2024	recurring hypoglycemia	2003:2024	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	7	83	theme	E-2	1167:1169	arg1	response					1187:1194	E-2 chromatographic response	1167:1194	E-2 chromatographic response	1167:1194	E-2 chromatographic response was further enhanced by optimization of solid phase extraction and instrument source and collision-induced dissociation voltages.					
32966939	3	84	theme	high-resolution	395:409	arg1	tools					433:437	high-resolution micropunch dissection tools	395:437	high-resolution micropunch dissection tools for isolation of segmental VMN tissue	395:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	9	85	dep	acute	1557:1561	arg1	VMN					1606:1608	caudal VMN	1599:1608	caudal VMN	1599:1608	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	85	dep	acute	1557:1561	arg1	VMN					1583:1585	increased, rostral VMN	1564:1585	VMN	1583:1585	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	4	86	theme	Composite	818:826	arg1	CCD					836:838	CCD	836:838	CCD	836:838	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	4	86	theme	Composite	818:826	arg1	Design					828:833	Central Composite Design	810:833	Central Composite Design (CCD)	810:839	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	11	87	theme	profiles	1991:1998	arg1	acclimation					1972:1982	VMN rostro-caudal segment-specific acclimation	1937:1982	VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia	1937:2024	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	0	88	theme	Estradiol	147:155	arg1	Concentrations					157:170	Brain Estradiol Concentrations	141:170	Brain Estradiol Concentrations	141:170	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	1	89	theme	glucostasis	224:234	arg1	control					213:219	Ventromedial hypothalamic nucleus (VMN) control	173:219	Ventromedial hypothalamic nucleus (VMN) control of glucostasis	173:234	Ventromedial hypothalamic nucleus (VMN) control of glucostasis is estradiol (E-2)-dependent.					
32966939	9	90	theme	regional	1506:1513	arg1	variability					1515:1525	regional variability	1506:1525	regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1506:1684	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	91	theme	rostral	1637:1643	arg1	change					1629:1634	no change	1626:1634	no change	1626:1634	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	9	91	theme	rostral	1637:1643	arg1	VMN					1645:1647	rostral VMN	1637:1647	rostral VMN	1637:1647	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	3	92	theme	dissection	422:431	arg1	tools					433:437	high-resolution micropunch dissection tools	395:437	high-resolution micropunch dissection tools for isolation of segmental VMN tissue	395:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	3	93	theme	uHPLC-electrospray	522:539	arg1	spectrometry					557:568	uHPLC-electrospray ionization-mass spectrometry	522:568	Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	502:592	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	3	93	theme	uHPLC-electrospray	522:539	arg1	LC-ESI-MS					571:579	LC-ESI-MS	571:579	LC-ESI-MS	571:579	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	0	94	theme	Liquid	23:28	arg1	Spectrometry					74:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry	0:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.	0:171	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	9	95	theme	E-2	1537:1539	arg1	profiles					1541:1548	tissue E-2 profiles	1530:1548	tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia	1530:1684	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	3	96	theme	VMN	466:468	arg1	tissue					470:475	segmental VMN tissue	456:475	segmental VMN tissue	456:475	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	4	97	theme	chromatogram	856:867	arg1	area					869:872	E-2 chromatogram area	852:872	E-2 chromatogram area	852:872	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	0	98	theme	Ionization-Mass	58:72	arg1	Spectrometry					74:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry	0:85	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.	0:171	Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for High-Neuroanatomical Resolution Quantification of Brain Estradiol Concentrations.					
32966939	1	99	theme	Ventromedial	173:184	arg1	VMN					208:210	VMN	208:210	VMN	208:210	Ventromedial hypothalamic nucleus (VMN) control of glucostasis is estradiol (E-2)-dependent.					
32966939	1	99	theme	Ventromedial	173:184	arg1	nucleus					199:205	Ventromedial hypothalamic nucleus	173:205	Ventromedial hypothalamic nucleus (VMN) control of glucostasis	173:234	Ventromedial hypothalamic nucleus (VMN) control of glucostasis is estradiol (E-2)-dependent.					
32966939	5	100	theme	gas	921:923	arg1	pressure					925:932	gas pressure	921:932	gas pressure	921:932	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	6	101	theme	Fibonacci	1025:1033	arg1	design					1044:1049	A Fibonacci Sequence design	1023:1049	A Fibonacci Sequence design using CCD minimum and maximal variable limits	1023:1095	A Fibonacci Sequence design using CCD minimum and maximal variable limits produced a small-run model that replicated maximal response from CCD.					
32966939	10	102	theme	high-neuroanatomical	1814:1833	arg1	specificity					1835:1845	high-neuroanatomical specificity	1814:1845	high-neuroanatomical specificity	1814:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
32966939	11	103	theme	physiological	1907:1919	arg1	consequences					1921:1932	physiological consequences	1907:1932	physiological consequences	1907:1932	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	11	103	theme	physiological	1907:1919	arg1	effects					2037:2043	effects	2037:2043	effects on gluco-regulatory function	2037:2072	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	9	104	dep	change	1629:1634	arg1	VMN					1668:1670	increased, caudal VMN	1650:1670	VMN	1668:1670	However, females exhibited regional variability in tissue E-2 profiles during acute (increased, rostral VMN; no change, caudal VMN) and recurring (no change, rostral VMN; increased, caudal VMN) hypoglycemia.					
32966939	3	105	theme	spectrometry	557:568	arg1	methodology					582:592	Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	502:592	Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	502:592	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	5	106	theme	variable	934:941	arg1	combinations					943:954	variable combinations	934:954	variable combinations	934:954	Combinations of spectrometric temperature and gas pressure variable combinations were screened by Akaike Information Criterion correction modeling.					
32966939	8	107	theme	male	1329:1332	arg1	rats					1334:1337	male rats	1329:1337	male rats	1329:1337	In male rats, acute and chronic hypoglycemia respectively elevated or diminished E-2 concentrations relative to baseline in both rostral and caudal VMN.					
32966939	4	108	dep	spectrometric	757:769	arg1	operational					771:781	operational	771:781	operational	771:781	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	11	109	theme	acclimation	1972:1982	arg1	consequences					1921:1932	physiological consequences	1907:1932	physiological consequences	1907:1932	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	11	109	theme	acclimation	1972:1982	arg1	effects					2037:2043	effects	2037:2043	effects on gluco-regulatory function	2037:2072	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	11	110	theme	rostro-caudal	1941:1953	arg1	acclimation					1972:1982	VMN rostro-caudal segment-specific acclimation	1937:1982	VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia	1937:2024	Current methodology will facilitate efforts to investigate physiological consequences of VMN rostro-caudal segment-specific acclimation of E-2 profiles to recurring hypoglycemia, including effects on gluco-regulatory function, in each sex.					
32966939	3	111	theme	methodology	582:592	arg1	Design					492:497	Design	492:497	Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology	492:592	Here, high-resolution micropunch dissection tools for isolation of segmental VMN tissue were used with Design of Experiments-refined uHPLC-electrospray ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the premise that effects of acute and/or recurring hypoglycemia on VMN E-2 content are sex-dimorphic.					
32966939	4	112	theme	independent	740:750	arg1	variables					783:791	multiple independent mass spectrometric operational variables	731:791	multiple independent mass spectrometric operational variables	731:791	Relationships among multiple independent mass spectrometric operational variables were assessed by Central Composite Design (CCD) to amplify E-2 chromatogram area.					
32966939	10	113	theme	LC-ESI-MS	1718:1726	arg1	sensitivity					1728:1738	requisite LC-ESI-MS sensitivity	1708:1738	requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity	1708:1845	Outcomes demonstrate requisite LC-ESI-MS sensitivity for E-2 quantification in small-volume brain tissue samples acquired with high-neuroanatomical specificity.					
33453255	6	0	theme	release	942:948	arg1	behaviors					950:958	the release behaviors	938:958	the release behaviors of monascus yellow entrapped in the hydrogel network	938:1011	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	1	1	theme	present	149:155	arg1	study					157:161	the present study	145:161	the present study	145:161	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	2	theme	KC	223:224	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	2	theme	KC	223:224	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	2	theme	KC	223:224	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	7	3	theme	promising	1106:1114	arg1	strategy					1116:1123	a promising strategy	1104:1123	a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions	1104:1205	Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.					
33453255	5	4	theme	compact	869:875	arg1	tougher					843:849	tougher	843:849	tougher	843:849	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	5	4	theme	compact	869:875	arg1	gels					887:890	more uniform and compact composite gels	852:890	more uniform and compact composite gels with sustained-release property	852:922	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	1	5	theme	study	157:161	arg1	aim					138:140	The aim	134:140	The aim of the present study	134:161	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	6	6	theme	entrapped	979:987	arg1	behaviors					950:958	the release behaviors	938:958	the release behaviors of monascus yellow entrapped in the hydrogel network	938:1011	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	7	7	theme	digestive	1186:1194	arg1	conditions					1196:1205	digestive conditions	1186:1205	digestive conditions	1186:1205	Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.					
33453255	6	8	theme	hydrogel	996:1003	arg1	network					1005:1011	the hydrogel network	992:1011	the hydrogel network	992:1011	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	3	9	dep	properties	439:448	arg1	the					396:398	the	396:398	the	396:398	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	3	10	theme	gels	525:528	arg1	yellow					501:506	monascus yellow	492:506	monascus yellow of the composite gels	492:528	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	1	11	theme	composite	227:235	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	11	theme	composite	227:235	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	11	theme	composite	227:235	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	0	12	theme	hydrophilic	94:104	arg1	compounds					106:114	hydrophilic compounds	94:114	hydrophilic compounds	94:114	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	4	13	theme	hydrogels	734:742	arg1	formation					704:712	the formation	700:712	the formation of SPI/KC composite hydrogels	700:742	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	2	14	used	used	330:333	arg2	model					340:344	a model	338:344	a model	338:344	The pigment of monascus yellow was used as a model.					
33453255	2	14	used	used	330:333	arg2	pigment					299:305	The pigment	295:305	The pigment of monascus yellow	295:324	The pigment of monascus yellow was used as a model.					
33453255	3	15	theme	digestion	463:471	arg1	profile					481:487	in vitro digestion release profile	454:487	in vitro digestion release profile	454:487	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	4	16	theme	power	546:550	arg1	law					552:554	power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR)	546:640	law	552:554	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	0	17	theme	Monascus	117:124	arg1	yellow					126:131	Monascus yellow	117:131	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.	0:132	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	3	18	theme	in	454:455	arg1	profile					481:487	in vitro digestion release profile	454:487	in vitro digestion release profile	454:487	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	3	19	theme	monascus	492:499	arg1	yellow					501:506	monascus yellow	492:506	monascus yellow of the composite gels	492:528	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	6	20	theme	monascus	963:970	arg1	entrapped					979:987	monascus yellow entrapped	963:987	monascus yellow entrapped	963:987	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	5	21	theme	composite	877:885	arg1	tougher					843:849	tougher	843:849	tougher	843:849	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	5	21	theme	composite	877:885	arg1	gels					887:890	more uniform and compact composite gels	852:890	more uniform and compact composite gels with sustained-release property	852:922	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	5	22	theme	pure	757:760	arg1	hydrogels					776:784	pure κ-carrageenan hydrogels	757:784	pure κ-carrageenan hydrogels	757:784	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	3	23	theme	yellow	501:506	arg1	properties					439:448	properties	439:448	properties	439:448	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	3	23	theme	yellow	501:506	arg1	profile					481:487	in vitro digestion release profile	454:487	in vitro digestion release profile	454:487	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	3	24	theme	rheological	400:410	arg1	properties					439:448	properties	439:448	properties	439:448	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	5	25	with	gels	887:890	arg1	property					915:922	sustained-release property	897:922	sustained-release property	897:922	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	6	26	theme	Ritger-Peppas	1042:1054	arg1	model					1069:1073	the Ritger-Peppas mathematical model	1038:1073	the Ritger-Peppas mathematical model	1038:1073	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	1	27	theme	soy	178:180	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	27	theme	soy	178:180	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	27	theme	soy	178:180	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	0	28	theme	protein	19:25	arg1	hydrogels					59:67	soy protein isolate/κ-carrageenan composite hydrogels	15:67	soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds	15:114	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	6	29	theme	mathematical	1056:1067	arg1	model					1069:1073	the Ritger-Peppas mathematical model	1038:1073	the Ritger-Peppas mathematical model	1038:1073	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	3	30	theme	composite	515:523	arg1	gels					525:528	the composite gels	511:528	the composite gels	511:528	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	1	31	theme	protein	182:188	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	31	theme	protein	182:188	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	31	theme	protein	182:188	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	0	32	theme	soy	15:17	arg1	hydrogels					59:67	soy protein isolate/κ-carrageenan composite hydrogels	15:67	soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds	15:114	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	1	33	theme	delivery	252:259	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	33	theme	delivery	252:259	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	33	theme	delivery	252:259	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	3	34	dep	in	454:455	arg1	vitro					457:461	vitro	457:461	vitro	457:461	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	4	35	dep	law	552:554	arg1	modeling					556:563	modeling	556:563	modeling	556:563	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	2	36	theme	yellow	319:324	arg1	pigment					299:305	The pigment	295:305	The pigment of monascus yellow	295:324	The pigment of monascus yellow was used as a model.					
33453255	2	36	theme	yellow	319:324	arg1	model					340:344	a model	338:344	a model	338:344	The pigment of monascus yellow was used as a model.					
33453255	1	37	theme	isolate	190:196	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	37	theme	isolate	190:196	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	37	theme	isolate	190:196	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	0	38	theme	composite	49:57	arg1	hydrogels					59:67	soy protein isolate/κ-carrageenan composite hydrogels	15:67	soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds	15:114	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	4	39	theme	non-covalent	657:668	arg1	interactions					670:681	non-covalent interactions	657:681	non-covalent interactions	657:681	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	0	40	theme	isolate/κ-carrageenan	27:47	arg1	hydrogels					59:67	soy protein isolate/κ-carrageenan composite hydrogels	15:67	soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds	15:114	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	5	41	theme	κ-carrageenan	762:774	arg1	hydrogels					776:784	pure κ-carrageenan hydrogels	757:784	pure κ-carrageenan hydrogels	757:784	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	4	42	theme	patterns	582:589	arg1	results					535:541	The results	531:541	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR)	531:640	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	6	43	from	behaviors	950:958	arg1	network					1005:1011	the hydrogel network	992:1011	the hydrogel network	992:1011	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	3	44	theme	systematic	349:358	arg1	study					360:364	A systematic study	347:364	A systematic study	347:364	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	1	45	theme	hydrophilic	272:282	arg1	compounds					284:292	hydrophilic compounds	272:292	hydrophilic compounds	272:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	4	46	dep	fourier	595:601	arg1	transform					603:611	transform	603:611	transform infrared spectroscopy (FTIR)	603:640	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	7	47	from	performance	1158:1168	arg1	conditions					1196:1205	digestive conditions	1186:1205	digestive conditions	1186:1205	Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.					
33453255	2	48	theme	monascus	310:317	arg1	yellow					319:324	monascus yellow	310:324	monascus yellow	310:324	The pigment of monascus yellow was used as a model.					
33453255	0	49	theme	hydrogels	59:67	arg1	Development					0:10	Development	0:10	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.	0:132	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	3	50	dep	rheological	400:410	arg1	textural					413:420	textural	413:420	textural	413:420	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	3	50	dep	rheological	400:410	arg1	microstructural					423:437	microstructural	423:437	microstructural	423:437	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	3	51	theme	release	473:479	arg1	profile					481:487	in vitro digestion release profile	454:487	in vitro digestion release profile	454:487	A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels.					
33453255	7	52	theme	sustained	1140:1148	arg1	performance					1158:1168	the sustained release performance	1136:1168	the sustained release performance of hydrogels in digestive conditions	1136:1205	Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.					
33453255	4	53	dep	transform	603:611	arg1	infrared					613:620	infrared	613:620	transform infrared spectroscopy (FTIR)	603:640	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	5	54	theme	sustained-release	897:913	arg1	property					915:922	sustained-release property	897:922	sustained-release property	897:922	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	4	55	theme	composite	724:732	arg1	hydrogels					734:742	SPI/KC composite hydrogels	717:742	SPI/KC composite hydrogels	717:742	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	6	56	theme	yellow	972:977	arg1	entrapped					979:987	monascus yellow entrapped	963:987	monascus yellow entrapped	963:987	In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model.					
33453255	5	57	theme	SPI	808:810	arg1	incorporation					791:803	the incorporation	787:803	the incorporation of SPI	787:810	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	4	58	theme	SPI/KC	717:722	arg1	hydrogels					734:742	SPI/KC composite hydrogels	717:742	SPI/KC composite hydrogels	717:742	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	0	59	theme	delivery	74:81	arg1	system					83:88	a delivery system	72:88	a delivery system for hydrophilic compounds	72:114	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	4	60	theme	fourier	595:601	arg1	results					535:541	The results	531:541	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR)	531:640	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	5	61	theme	uniform	857:863	arg1	tougher					843:849	tougher	843:849	tougher	843:849	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	5	61	theme	uniform	857:863	arg1	gels					887:890	more uniform and compact composite gels	852:890	more uniform and compact composite gels with sustained-release property	852:922	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	5	62	theme	tougher	843:849	arg1	formation					830:838	the formation	826:838	the formation of tougher, more uniform and compact composite gels with sustained-release property	826:922	Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property.					
33453255	1	63	theme	κ-carrageenan	208:220	arg1	SPI					199:201	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	SPI	199:201	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	63	theme	κ-carrageenan	208:220	arg1	system					261:266	a delivery system	250:266	a delivery system for hydrophilic compounds	250:292	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	1	63	theme	κ-carrageenan	208:220	arg1	hydrogels					237:245	soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels	178:245	hydrogels	237:245	The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds.					
33453255	4	64	theme	law	552:554	arg1	results					535:541	The results	531:541	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR)	531:640	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
33453255	7	65	theme	hydrogels	1173:1181	arg1	performance					1158:1168	the sustained release performance	1136:1168	the sustained release performance of hydrogels in digestive conditions	1136:1205	Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.					
33453255	0	66	dep	Development	0:10	arg1	yellow					126:131	Monascus yellow	117:131	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.	0:132	Development of soy protein isolate/κ-carrageenan composite hydrogels as a delivery system for hydrophilic compounds: Monascus yellow.					
33453255	7	67	theme	release	1150:1156	arg1	performance					1158:1168	the sustained release performance	1136:1168	the sustained release performance of hydrogels in digestive conditions	1136:1205	Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.					
33453255	4	68	theme	electrophoresis	566:580	arg1	patterns					582:589	electrophoresis patterns	566:589	electrophoresis patterns	566:589	The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels.					
34973756	3	0	theme	cation-selective	308:323	arg1	membrane					338:345	a cation-selective and low-cost membrane	306:345	a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups	306:502	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	5	1	theme	resulting	740:748	arg1	properties					764:773	the resulting ion transport properties	736:773	the resulting ion transport properties	736:773	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	1	2	theme	harvesting	144:153	arg1	heart					113:117	the heart	109:117	the heart of energy conversion and harvesting, water treatment, and biotechnologies	109:191	Ion selective membranes are at the heart of energy conversion and harvesting, water treatment, and biotechnologies.					
34973756	6	3	theme	higher	821:826	arg1	content					840:846	higher crosslinker content	821:846	higher crosslinker content	821:846	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
34973756	0	4	from	effect	4:9	arg1	transport					34:42	ion transport	30:42	ion transport in nanocellulose-based membranes	30:75	The effect of crosslinking on ion transport in nanocellulose-based membranes.					
34973756	7	5	theme	%	995:995	arg1	addition					1009:1016	20 wt% crosslinker addition	990:1016	20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm)	990:1053	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	7	6	theme	ion	922:924	arg1	selectivity					926:936	Optimal ion selectivity	914:936	Optimal ion selectivity	914:936	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	3	7	theme	renewable	361:369	arg1	nanocellulose					371:383	renewable nanocellulose	361:383	renewable nanocellulose	361:383	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	8	8	theme	regular	1059:1065	arg1	nanochannels					1075:1086	regular ~1.4 nm nanochannels	1059:1086	regular ~1.4 nm nanochannels	1059:1086	As regular ~1.4 nm nanochannels were formed at this composition, nanofluidic contribution to ion transport is likely.					
34973756	5	9	theme	nanochannel	643:653	arg1	structure					655:663	the nanochannel structure	639:663	the nanochannel structure	639:663	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	5	10	theme	different	573:581	arg1	degrees					583:589	different degrees	573:589	different degrees of crosslinking	573:605	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	7	11	theme	mS/cm	1048:1052	arg1	conductivity					1028:1039	ion conductivity	1024:1039	ion conductivity of 1.6 mS/cm	1024:1052	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	0	12	from	transport	34:42	arg1	membranes					67:75	nanocellulose-based membranes	47:75	nanocellulose-based membranes	47:75	The effect of crosslinking on ion transport in nanocellulose-based membranes.					
34973756	5	13	theme	surface	669:675	arg1	concentration					684:696	surface charge concentration	669:696	surface charge concentration	669:696	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	7	14	theme	20 wt	990:994	arg1	addition					1009:1016	20 wt% crosslinker addition	990:1016	20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm)	990:1053	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	7	15	theme	Optimal	914:920	arg1	selectivity					926:936	Optimal ion selectivity	914:936	Optimal ion selectivity	914:936	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	1	16	theme	water	156:160	arg1	treatment					162:170	water treatment	156:170	water treatment	156:170	Ion selective membranes are at the heart of energy conversion and harvesting, water treatment, and biotechnologies.					
34973756	5	17	theme	charge	677:682	arg1	concentration					684:696	surface charge concentration	669:696	surface charge concentration	669:696	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	5	18	theme	simultaneous	608:619	arg1	control					621:627	simultaneous control	608:627	simultaneous control over both the nanochannel structure and surface charge concentration	608:696	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	1	19	theme	Ion	78:80	arg1	membranes					92:100	Ion selective membranes	78:100	Ion selective membranes	78:100	Ion selective membranes are at the heart of energy conversion and harvesting, water treatment, and biotechnologies.					
34973756	3	20	theme	anionic	481:487	arg1	groups					497:502	anionic surface groups	481:502	anionic surface groups	481:502	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	6	21	theme	obtained	853:860	arg1	conductivity					866:877	the obtained ion conductivity	849:877	the obtained ion conductivity	849:877	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
34973756	1	22	theme	selective	82:90	arg1	membranes					92:100	Ion selective membranes	78:100	Ion selective membranes	78:100	Ion selective membranes are at the heart of energy conversion and harvesting, water treatment, and biotechnologies.					
34973756	0	23	theme	crosslinking	14:25	arg1	effect					4:9	The effect	0:9	The effect of crosslinking on ion transport in nanocellulose-based membranes	0:75	The effect of crosslinking on ion transport in nanocellulose-based membranes.					
34973756	6	24	dep	8	893:893	arg1	to					890:891	to	890:891	to	890:891	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
34973756	5	25	theme	ion	750:752	arg1	properties					764:773	the resulting ion transport properties	736:773	the resulting ion transport properties	736:773	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	3	26	theme	1,2,3,4-butanetetracarboxylic	389:417	arg1	acid					419:422	1,2,3,4-butanetetracarboxylic acid	389:422	1,2,3,4-butanetetracarboxylic acid	389:422	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	5	27	theme	transport	754:762	arg1	properties					764:773	the resulting ion transport properties	736:773	the resulting ion transport properties	736:773	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	0	28	theme	ion	30:32	arg1	transport					34:42	ion transport	30:42	ion transport in nanocellulose-based membranes	30:75	The effect of crosslinking on ion transport in nanocellulose-based membranes.					
34973756	3	29	theme	surface	489:495	arg1	groups					497:502	anionic surface groups	481:502	anionic surface groups	481:502	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	4	30	theme	Charge	505:510	arg1	density					512:518	Charge density	505:518	Charge density	505:518	Charge density and structure of the membranes are studied.					
34973756	3	31	theme	groups	497:502	arg1	source					471:476	source	471:476	source	471:476	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	3	31	theme	groups	497:502	arg1	crosslinker					455:465	crosslinker	455:465	crosslinker	455:465	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	6	32	theme	crosslinker	828:838	arg1	content					840:846	higher crosslinker content	821:846	higher crosslinker content	821:846	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
34973756	8	33	theme	nanofluidic	1121:1131	arg1	contribution					1133:1144	nanofluidic contribution	1121:1144	nanofluidic contribution to ion transport	1121:1161	As regular ~1.4 nm nanochannels were formed at this composition, nanofluidic contribution to ion transport is likely.					
34973756	2	34	theme	available	208:216	arg1	membranes					218:226	The currently available membranes	194:226	The currently available membranes	194:226	The currently available membranes are mostly based on expensive and non-biodegradable polymers.					
34973756	5	35	theme	crosslinking	594:605	arg1	degrees					583:589	different degrees	573:589	different degrees of crosslinking	573:605	By using different degrees of crosslinking, simultaneous control over both the nanochannel structure and surface charge concentration is achieved, which in turn determines the resulting ion transport properties.					
34973756	8	36	theme	~1.4 nm	1067:1073	arg1	nanochannels					1075:1086	regular ~1.4 nm nanochannels	1059:1086	regular ~1.4 nm nanochannels	1059:1086	As regular ~1.4 nm nanochannels were formed at this composition, nanofluidic contribution to ion transport is likely.					
34973756	7	37	theme	ion	1024:1026	arg1	conductivity					1028:1039	ion conductivity	1024:1039	ion conductivity of 1.6 mS/cm	1024:1052	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	8	38	theme	ion	1149:1151	arg1	transport					1153:1161	ion transport	1149:1161	ion transport	1149:1161	As regular ~1.4 nm nanochannels were formed at this composition, nanofluidic contribution to ion transport is likely.					
34973756	6	39	theme	ion	862:864	arg1	conductivity					866:877	the obtained ion conductivity	849:877	the obtained ion conductivity	849:877	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
34973756	7	40	theme	crosslinker	997:1007	arg1	addition					1009:1016	20 wt% crosslinker addition	990:1016	20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm)	990:1053	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	7	41	theme	solution	962:969	arg1	pH					971:972	the solution pH	958:972	the solution pH	958:972	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	7	42	with	addition	1009:1016	arg1	conductivity					1028:1039	ion conductivity	1024:1039	ion conductivity of 1.6 mS/cm	1024:1052	Optimal ion selectivity, also influenced by the solution pH, is achieved at 20 wt% crosslinker addition (with ion conductivity of 1.6 mS/cm).					
34973756	2	43	theme	non-biodegradable	262:278	arg1	polymers					280:287	expensive and non-biodegradable polymers	248:287	expensive and non-biodegradable polymers	248:287	The currently available membranes are mostly based on expensive and non-biodegradable polymers.					
34973756	2	44	theme	expensive	248:256	arg1	polymers					280:287	expensive and non-biodegradable polymers	248:287	expensive and non-biodegradable polymers	248:287	The currently available membranes are mostly based on expensive and non-biodegradable polymers.					
34973756	1	45	theme	energy	122:127	arg1	conversion					129:138	energy conversion	122:138	energy conversion	122:138	Ion selective membranes are at the heart of energy conversion and harvesting, water treatment, and biotechnologies.					
34973756	3	46	theme	low-cost	329:336	arg1	membrane					338:345	a cation-selective and low-cost membrane	306:345	a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups	306:502	Here, we report a cation-selective and low-cost membrane prepared from renewable nanocellulose and 1,2,3,4-butanetetracarboxylic acid which simultaneously serves as crosslinker and source of anionic surface groups.					
34973756	6	47	theme	charge	796:801	arg1	concentration					803:815	negative charge concentration	787:815	negative charge concentration	787:815	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
34973756	1	48	theme	conversion	129:138	arg1	heart					113:117	the heart	109:117	the heart of energy conversion and harvesting, water treatment, and biotechnologies	109:191	Ion selective membranes are at the heart of energy conversion and harvesting, water treatment, and biotechnologies.					
34973756	0	49	theme	nanocellulose-based	47:65	arg1	membranes					67:75	nanocellulose-based membranes	47:75	nanocellulose-based membranes	47:75	The effect of crosslinking on ion transport in nanocellulose-based membranes.					
34973756	4	50	theme	membranes	541:549	arg1	structure					524:532	structure	524:532	structure	524:532	Charge density and structure of the membranes are studied.					
34973756	4	50	theme	membranes	541:549	arg1	density					512:518	Charge density	505:518	Charge density	505:518	Charge density and structure of the membranes are studied.					
34973756	6	51	theme	negative	787:794	arg1	concentration					803:815	negative charge concentration	787:815	negative charge concentration	787:815	Increasing negative charge concentration via higher crosslinker content, the obtained ion conductivity reaches up to 8 mS/cm (0.1 M KCl).					
33724030	0	0	from	Bifidobacteria	30:43	arg1	Diet					87:90	a High-Fat Diet	76:90	a High-Fat Diet	76:90	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	0	0	from	Bifidobacteria	30:43	arg1	Mice					68:71	Mice	68:71	Mice	68:71	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	1	1	from	Effects	174:180	arg1	mice					308:311	mice	308:311	mice fed a high-fat diet (HFD)	308:337	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	5	2	theme	cecum	756:760	arg1	microbiota					762:771	The cecum microbiota	752:771	The cecum microbiota of the XOS group	752:788	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	8	3	theme	XOS	1119:1121	arg1	diet					1123:1126	the XOS diet	1115:1126	the XOS diet	1115:1126	The fiber diversity in the MIX diet did not provide any advantages compared to the XOS diet.					
33724030	2	4	dep	HFD	380:382	arg1	an					377:378	an	377:378	an	377:378	Control groups were fed an HFD or a low-fat diet.					
33724030	8	5	theme	fiber	1040:1044	arg1	diversity					1046:1054	The fiber diversity	1036:1054	The fiber diversity in the MIX diet	1036:1070	The fiber diversity in the MIX diet did not provide any advantages compared to the XOS diet.					
33724030	4	6	theme	cecum	605:609	arg1	levels					611:616	significantly higher cecum levels	584:616	significantly higher cecum levels of acetic, propionic, and butyric acids	584:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	1	7	theme	XOS	237:239	arg1	XOSs					207:210	XOSs	207:210	XOSs	207:210	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	7	theme	XOS	237:239	arg1	xylooligosaccharides					185:204	xylooligosaccharides	185:204	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	7	theme	XOS	237:239	arg1	mixture					226:232	a mixture	224:232	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	7	8	theme	prebiotic	1015:1023	arg1	XOS					983:985	the XOS	979:985	the XOS	979:985	In conclusion, the XOS was shown to be a promising prebiotic candidate.					
33724030	7	8	theme	prebiotic	1015:1023	arg1	candidate					1025:1033	a promising prebiotic candidate	1003:1033	a promising prebiotic candidate	1003:1033	In conclusion, the XOS was shown to be a promising prebiotic candidate.					
33724030	5	9	theme	Lachnospiraceae	821:835	arg1	bacteria					848:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	4	10	theme	butyric	644:650	arg1	acids					652:656	acetic, propionic, and butyric acids	621:656	acetic, propionic, and butyric acids	621:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	8	11	from	diversity	1046:1054	arg1	diet					1067:1070	the MIX diet	1059:1070	the MIX diet	1059:1070	The fiber diversity in the MIX diet did not provide any advantages compared to the XOS diet.					
33724030	4	12	theme	MIX	741:743	arg1	group					745:749	the MIX group	737:749	the MIX group	737:749	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	1	13	theme	inulin	242:247	arg1	XOSs					207:210	XOSs	207:210	XOSs	207:210	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	13	theme	inulin	242:247	arg1	xylooligosaccharides					185:204	xylooligosaccharides	185:204	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	13	theme	inulin	242:247	arg1	mixture					226:232	a mixture	224:232	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	4	14	theme	acetic	621:626	arg1	acids					652:656	acetic, propionic, and butyric acids	621:656	acetic, propionic, and butyric acids	621:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	3	15	theme	cecal	437:441	arg1	composition					443:453	the cecal composition	433:453	the cecal composition of the gut microbiota and formation of short-chain fatty acids	433:516	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	4	16	theme	XOS	570:572	arg1	group					574:578	The XOS group	566:578	The XOS group	566:578	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	0	17	theme	Concomitant	100:110	arg1	Increase					112:119	a Concomitant Increase	98:119	a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid	98:171	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	4	18	theme	HFD	667:669	arg1	group					671:675	the HFD group	663:675	the HFD group	663:675	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	0	19	from	Lachnospiraceae	49:63	arg1	Diet					87:90	a High-Fat Diet	76:90	a High-Fat Diet	76:90	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	0	19	from	Lachnospiraceae	49:63	arg1	Mice					68:71	Mice	68:71	Mice	68:71	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	8	20	theme	MIX	1063:1065	arg1	diet					1067:1070	the MIX diet	1059:1070	the MIX diet	1059:1070	The fiber diversity in the MIX diet did not provide any advantages compared to the XOS diet.					
33724030	3	21	theme	formation	481:489	arg1	composition					443:453	the cecal composition	433:453	the cecal composition of the gut microbiota and formation of short-chain fatty acids	433:516	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	1	22	theme	xylooligosaccharides	185:204	arg1	Effects					174:180	Effects	174:180	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD)	174:337	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	23	theme	oligofructose	250:262	arg1	XOSs					207:210	XOSs	207:210	XOSs	207:210	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	23	theme	oligofructose	250:262	arg1	xylooligosaccharides					185:204	xylooligosaccharides	185:204	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	23	theme	oligofructose	250:262	arg1	mixture					226:232	a mixture	224:232	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	0	24	theme	Short-Chain	124:134	arg1	Acids					142:146	Short-Chain Fatty Acids	124:146	Short-Chain Fatty Acids	124:146	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	0	24	theme	Short-Chain	124:134	arg1	Acid					168:171	Butyric Acid	160:171	Butyric Acid	160:171	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	3	25	theme	gut	462:464	arg1	microbiota					466:475	the gut microbiota and formation	458:489	microbiota	466:475	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	2	26	theme	Control	353:359	arg1	groups					361:366	Control groups	353:366	Control groups	353:366	Control groups were fed an HFD or a low-fat diet.					
33724030	6	27	theme	HFD	952:954	arg1	groups					956:961	the XOS and HFD groups	940:961	the XOS and HFD groups	940:961	A tendency of lower body weight gain was observed on comparing the XOS and HFD groups.					
33724030	3	28	theme	microbiota	466:475	arg1	composition					443:453	the cecal composition	433:453	the cecal composition of the gut microbiota and formation of short-chain fatty acids	433:516	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	6	29	theme	body	897:900	arg1	gain					909:912	lower body weight gain	891:912	lower body weight gain	891:912	A tendency of lower body weight gain was observed on comparing the XOS and HFD groups.					
33724030	2	30	theme	low-fat	389:395	arg1	diet					397:400	a low-fat diet	387:400	a low-fat diet	387:400	Control groups were fed an HFD or a low-fat diet.					
33724030	3	31	theme	fatty	506:510	arg1	acids					512:516	short-chain fatty acids	494:516	short-chain fatty acids	494:516	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	6	32	theme	gain	909:912	arg1	tendency					879:886	A tendency	877:886	A tendency of lower body weight gain	877:912	A tendency of lower body weight gain was observed on comparing the XOS and HFD groups.					
33724030	6	33	theme	lower	891:895	arg1	gain					909:912	lower body weight gain	891:912	lower body weight gain	891:912	A tendency of lower body weight gain was observed on comparing the XOS and HFD groups.					
33724030	1	34	theme	high-fat	319:326	arg1	HFD					334:336	HFD	334:336	HFD	334:336	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	34	theme	high-fat	319:326	arg1	diet					328:331	a high-fat diet	317:331	a high-fat diet (HFD)	317:337	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	0	35	theme	Fatty	136:140	arg1	Acids					142:146	Short-Chain Fatty Acids	124:146	Short-Chain Fatty Acids	124:146	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	0	35	theme	Fatty	136:140	arg1	Acid					168:171	Butyric Acid	160:171	Butyric Acid	160:171	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	3	36	theme	acids	512:516	arg1	microbiota					466:475	the gut microbiota and formation	458:489	microbiota	466:475	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	3	36	theme	acids	512:516	arg1	formation					481:489	the gut microbiota and formation	458:489	formation	481:489	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	7	37	theme	promising	1005:1013	arg1	XOS					983:985	the XOS	979:985	the XOS	979:985	In conclusion, the XOS was shown to be a promising prebiotic candidate.					
33724030	7	37	theme	promising	1005:1013	arg1	candidate					1025:1033	a promising prebiotic candidate	1003:1033	a promising prebiotic candidate	1003:1033	In conclusion, the XOS was shown to be a promising prebiotic candidate.					
33724030	1	38	theme	hydrolyzed	279:288	arg1	MIX					300:302	MIX	300:302	MIX	300:302	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	38	theme	hydrolyzed	279:288	arg1	gum					295:297	partially hydrolyzed guar gum	269:297	partially hydrolyzed guar gum (MIX)	269:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	3	39	theme	Special	403:409	arg1	attention					411:419	Special attention	403:419	Special attention	403:419	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	5	40	theme	Bifidobacteria	805:818	arg1	bacteria					848:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	1	41	theme	guar	290:293	arg1	MIX					300:302	MIX	300:302	MIX	300:302	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	41	theme	guar	290:293	arg1	gum					295:297	partially hydrolyzed guar gum	269:297	partially hydrolyzed guar gum (MIX)	269:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	5	42	dep	more	800:803	arg1	bacteria					848:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	0	43	from	Increase	112:119	arg1	Acids					142:146	Short-Chain Fatty Acids	124:146	Short-Chain Fatty Acids	124:146	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	0	43	from	Increase	112:119	arg1	Acid					168:171	Butyric Acid	160:171	Butyric Acid	160:171	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	3	44	theme	short-chain	494:504	arg1	acids					512:516	short-chain fatty acids	494:516	short-chain fatty acids	494:516	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	1	45	theme	gum	295:297	arg1	XOSs					207:210	XOSs	207:210	XOSs	207:210	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	45	theme	gum	295:297	arg1	xylooligosaccharides					185:204	xylooligosaccharides	185:204	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	1	45	theme	gum	295:297	arg1	mixture					226:232	a mixture	224:232	xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX)	185:303	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
33724030	4	46	contain	had	580:582	arg2	levels					611:616	significantly higher cecum levels	584:616	significantly higher cecum levels of acetic, propionic, and butyric acids	584:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	4	46	contain	had	580:582	arg1	group					574:578	The XOS group	566:578	The XOS group	566:578	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	3	47	theme	metabolic	523:531	arg1	parameters					533:542	metabolic parameters	523:542	metabolic parameters	523:542	Special attention was paid to the cecal composition of the gut microbiota and formation of short-chain fatty acids, but metabolic parameters were also documented.					
33724030	4	48	theme	acid	694:697	arg1	content					699:705	the butyric acid content	682:705	the butyric acid content	682:705	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	4	48	theme	acid	694:697	arg1	higher					711:716	higher	711:716	higher	711:716	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	4	49	theme	propionic	629:637	arg1	acids					652:656	acetic, propionic, and butyric acids	621:656	acetic, propionic, and butyric acids	621:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	4	50	theme	acids	652:656	arg1	levels					611:616	significantly higher cecum levels	584:616	significantly higher cecum levels of acetic, propionic, and butyric acids	584:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	0	51	theme	Butyric	160:166	arg1	Acids					142:146	Short-Chain Fatty Acids	124:146	Short-Chain Fatty Acids	124:146	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	0	51	theme	Butyric	160:166	arg1	Acid					168:171	Butyric Acid	160:171	Butyric Acid	160:171	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	5	52	theme	group	784:788	arg1	microbiota					762:771	The cecum microbiota	752:771	The cecum microbiota of the XOS group	752:788	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	5	53	theme	XOS	780:782	arg1	group					784:788	the XOS group	776:788	the XOS group	776:788	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	0	54	theme	High-Fat	78:85	arg1	Diet					87:90	a High-Fat Diet	76:90	a High-Fat Diet	76:90	Xylooligosaccharides Increase Bifidobacteria and Lachnospiraceae in Mice on a High-Fat Diet, with a Concomitant Increase in Short-Chain Fatty Acids, Especially Butyric Acid.					
33724030	6	55	theme	XOS	944:946	arg1	groups					956:961	the XOS and HFD groups	940:961	the XOS and HFD groups	940:961	A tendency of lower body weight gain was observed on comparing the XOS and HFD groups.					
33724030	4	56	theme	butyric	686:692	arg1	content					699:705	the butyric acid content	682:705	the butyric acid content	682:705	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	4	56	theme	butyric	686:692	arg1	higher					711:716	higher	711:716	higher	711:716	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	6	57	theme	weight	902:907	arg1	gain					909:912	lower body weight gain	891:912	lower body weight gain	891:912	A tendency of lower body weight gain was observed on comparing the XOS and HFD groups.					
33724030	4	58	theme	higher	598:603	arg1	levels					611:616	significantly higher cecum levels	584:616	significantly higher cecum levels of acetic, propionic, and butyric acids	584:656	The XOS group had significantly higher cecum levels of acetic, propionic, and butyric acids than the HFD group, and the butyric acid content was higher in the XOS than in the MIX group.					
33724030	5	59	theme	HFD	866:868	arg1	group					870:874	the HFD group	862:874	the HFD group	862:874	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	5	60	theme	S24-7	842:846	arg1	bacteria					848:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	Bifidobacteria, Lachnospiraceae, and S24-7 bacteria	805:855	The cecum microbiota of the XOS group contained more Bifidobacteria, Lachnospiraceae, and S24-7 bacteria than the HFD group.					
33724030	1	61	theme	mixture	226:232	arg1	Effects					174:180	Effects	174:180	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD)	174:337	Effects of xylooligosaccharides (XOSs) as well as a mixture of XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed a high-fat diet (HFD) were studied.					
32518376	11	0	theme	brain-gut-microbiota	1832:1851	arg1	axis					1853:1856	the brain-gut-microbiota axis	1828:1856	the brain-gut-microbiota axis	1828:1856	It is likely that the vagus nerve plays a crucial role in the brain-gut-microbiota axis.					
32518376	4	1	from	increase	662:669	arg1	weight					681:686	spleen weight	674:686	spleen weight	674:686	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	3	2	theme	abnormal	514:521	arg1	composition					523:533	the abnormal composition	510:533	the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration	510:605	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	4	3	from	inflammation	648:659	arg1	weight					681:686	spleen weight	674:686	spleen weight	674:686	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	10	4	theme	gut	1717:1719	arg1	microbiota					1721:1730	gut microbiota	1717:1730	gut microbiota	1717:1730	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	4	5	from	phenotype	637:645	arg1	weight					681:686	spleen weight	674:686	spleen weight	674:686	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	1	6	theme	vagus	176:180	arg1	nerve					182:186	The vagus nerve	172:186	The vagus nerve	172:186	The vagus nerve plays a role in the cross talk between the brain and gut microbiota, which could be involved in depression.					
32518376	3	7	theme	microbiota	542:551	arg1	effects					430:436	the effects	426:436	the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype	426:504	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	3	7	theme	microbiota	542:551	arg1	composition					523:533	the abnormal composition	510:533	the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration	510:605	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	9	8	theme	sham + LPS	1430:1439	arg1	group					1441:1445	the sham + LPS group	1426:1445	the sham + LPS group	1426:1445	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	0	9	theme	gut	108:110	arg1	microbiota					112:121	gut microbiota	108:121	gut microbiota	108:121	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	10	10	theme	subdiaphragmatic	1740:1755	arg1	nerve					1763:1767	the subdiaphragmatic vagus nerve	1736:1767	the subdiaphragmatic vagus nerve	1736:1767	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	3	11	theme	lipopolysaccharide	567:584	arg1	administration					592:605	lipopolysaccharide (LPS) administration	567:605	lipopolysaccharide (LPS) administration	567:605	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	0	12	from	composition	93:103	arg1	mice					126:129	mice	126:129	mice after lipopolysaccharide administration	126:169	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	8	13	theme	gut	1298:1300	arg1	diversity					1313:1321	gut microbiota diversity	1298:1321	gut microbiota diversity in sham-operated mice	1298:1343	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	7	14	theme	inflammatory	1208:1219	arg1	events					1221:1226	inflammatory events	1208:1226	inflammatory events	1208:1226	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	6	15	theme	cytokines	971:979	arg1	lower					1009:1013	lower	1009:1013	lower	1009:1013	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	6	15	theme	cytokines	971:979	arg1	weight					926:931	The spleen weight and plasma levels	915:949	weight	926:931	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	6	15	theme	cytokines	971:979	arg1	levels					944:949	The spleen weight and plasma levels	915:949	levels	944:949	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	0	16	from	role	6:9	arg1	phenotype					70:78	depression-like phenotype	54:78	depression-like phenotype	54:78	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	0	16	from	role	6:9	arg1	composition					93:103	abnormal composition	84:103	abnormal composition	84:103	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	4	17	theme	spleen	674:679	arg1	weight					681:686	spleen weight	674:686	spleen weight	674:686	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	0	18	theme	depression-like	54:68	arg1	phenotype					70:78	depression-like phenotype	54:78	depression-like phenotype	54:78	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	4	19	from	cortex	754:759	arg1	mice					789:792	the sham-operated mice	771:792	the sham-operated mice	771:792	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	8	20	theme	sham-operated	1326:1338	arg1	mice					1340:1343	sham-operated mice	1326:1343	sham-operated mice	1326:1343	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	4	21	theme	proteins	720:727	arg1	phenotype					637:645	a depression-like phenotype	619:645	a depression-like phenotype	619:645	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	4	21	theme	proteins	720:727	arg1	inflammation					648:659	inflammation	648:659	inflammation	648:659	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	4	21	theme	proteins	720:727	arg1	increase					662:669	increase	662:669	increase in spleen weight	662:686	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	4	21	theme	proteins	720:727	arg1	downregulation					693:706	downregulation	693:706	downregulation of synaptic proteins	693:727	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	6	22	theme	plasma	937:942	arg1	levels					944:949	The spleen weight and plasma levels	915:949	levels	944:949	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	6	23	from	levels	944:949	arg1	group					998:1002	the SDV + LPS group	984:1002	the SDV + LPS group	984:1002	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	7	24	theme	weight	1166:1171	arg1	levels					1118:1123	the plasma levels	1107:1123	the plasma levels of pro-inflammatory cytokines and spleen weight	1107:1171	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	0	25	theme	subdiaphragmatic	18:33	arg1	nerve					41:45	the subdiaphragmatic vagus nerve	14:45	the subdiaphragmatic vagus nerve	14:45	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	8	26	from	diversity	1313:1321	arg1	mice					1340:1343	sham-operated mice	1326:1343	sham-operated mice	1326:1343	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	3	27	theme	depression-like	480:494	arg1	phenotype					496:504	the depression-like phenotype	476:504	the depression-like phenotype	476:504	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	4	28	theme	synaptic	711:718	arg1	proteins					720:727	synaptic proteins	711:727	synaptic proteins	711:727	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	7	29	theme	pro-inflammatory	1128:1143	arg1	cytokines					1145:1153	pro-inflammatory cytokines	1128:1153	pro-inflammatory cytokines	1128:1153	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	4	30	theme	sham-operated	775:787	arg1	mice					789:792	the sham-operated mice	771:792	the sham-operated mice	771:792	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	9	31	from	PCoA	1398:1401	arg1	dots					1408:1411	the dots	1404:1411	the dots representing the sham + LPS group	1404:1445	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	9	31	from	PCoA	1398:1401	arg1	located					1452:1458	located	1452:1458	located	1452:1458	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	9	32	from	located	1452:1458	arg1	PCoA					1398:1401	an unweighted UniFrac PCoA	1376:1401	an unweighted UniFrac PCoA	1376:1401	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	0	33	theme	vagus	35:39	arg1	nerve					41:45	the subdiaphragmatic vagus nerve	14:45	the subdiaphragmatic vagus nerve	14:45	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	2	34	theme	subdiaphragmatic	300:315	arg1	nerve					323:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	2	34	theme	subdiaphragmatic	300:315	arg1	pathway					358:364	a major modulatory pathway	339:364	a major modulatory pathway between the brain and gut microbiota	339:401	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	1	35	theme	cross	208:212	arg1	talk					214:217	the cross talk	204:217	the cross talk between the brain and gut microbiota, which could be involved in depression	204:293	The vagus nerve plays a role in the cross talk between the brain and gut microbiota, which could be involved in depression.					
32518376	10	36	from	proteins	1658:1665	arg1	mPFC					1674:1677	the mPFC	1670:1677	the mPFC	1670:1677	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	2	37	theme	major	341:345	arg1	nerve					323:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	2	37	theme	major	341:345	arg1	pathway					358:364	a major modulatory pathway	339:364	a major modulatory pathway between the brain and gut microbiota	339:401	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	7	38	theme	positive	1077:1084	arg1	correlations					1086:1097	positive correlations	1077:1097	positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight	1077:1171	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	10	39	theme	abnormal	1693:1700	arg1	composition					1702:1712	abnormal composition	1693:1712	abnormal composition of gut microbiota	1693:1730	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	9	40	theme	UniFrac	1390:1396	arg1	PCoA					1398:1401	an unweighted UniFrac PCoA	1376:1401	an unweighted UniFrac PCoA	1376:1401	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	10	41	theme	microbiota	1721:1730	arg1	composition					1702:1712	abnormal composition	1693:1712	abnormal composition of gut microbiota	1693:1730	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	4	42	theme	depression-like	621:635	arg1	phenotype					637:645	a depression-like phenotype	619:645	a depression-like phenotype	619:645	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	3	43	theme	gut	538:540	arg1	microbiota					542:551	gut microbiota	538:551	gut microbiota	538:551	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	9	44	located	located	1452:1458	arg1	PCoA					1398:1401	an unweighted UniFrac PCoA	1376:1401	an unweighted UniFrac PCoA	1376:1401	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	9	44	located	located	1452:1458	arg2	located					1452:1458	located	1452:1458	located	1452:1458	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	9	44	located	located	1452:1458	arg2	dots					1408:1411	the dots	1404:1411	the dots representing the sham + LPS group	1404:1445	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	10	45	theme	depression-like	1560:1574	arg1	phenotype					1576:1584	a depression-like phenotype	1558:1584	a depression-like phenotype	1558:1584	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	0	46	theme	microbiota	112:121	arg1	phenotype					70:78	depression-like phenotype	54:78	depression-like phenotype	54:78	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	0	46	theme	microbiota	112:121	arg1	composition					93:103	abnormal composition	84:103	abnormal composition	84:103	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	10	47	theme	vagus	1757:1761	arg1	nerve					1763:1767	the subdiaphragmatic vagus nerve	1736:1767	the subdiaphragmatic vagus nerve	1736:1767	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	8	48	from	alterations	1283:1293	arg1	diversity					1313:1321	gut microbiota diversity	1298:1321	gut microbiota diversity in sham-operated mice	1298:1343	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	6	49	theme	spleen	919:924	arg1	lower					1009:1013	lower	1009:1013	lower	1009:1013	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	6	49	theme	spleen	919:924	arg1	weight					926:931	The spleen weight and plasma levels	915:949	weight	926:931	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	0	50	from	phenotype	70:78	arg1	mice					126:129	mice	126:129	mice after lipopolysaccharide administration	126:169	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	10	51	theme	spleen	1597:1602	arg1	weight					1604:1609	spleen weight	1597:1609	spleen weight	1597:1609	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	0	52	theme	lipopolysaccharide	137:154	arg1	administration					156:169	lipopolysaccharide administration	137:169	lipopolysaccharide administration	137:169	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	5	53	theme	synaptic	874:881	arg1	proteins					883:890	synaptic proteins	874:890	synaptic proteins in the mPFC	874:902	In contrast, LPS did not produce a depression-like phenotype and downregulated synaptic proteins in the mPFC after SDV.					
32518376	3	54	from	composition	523:533	arg1	phenotype					496:504	the depression-like phenotype	476:504	the depression-like phenotype	476:504	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	3	54	from	composition	523:533	arg1	mice					556:559	mice	556:559	mice after lipopolysaccharide (LPS) administration	556:605	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	11	55	theme	vagus	1792:1796	arg1	nerve					1798:1802	the vagus nerve	1788:1802	the vagus nerve	1788:1802	It is likely that the vagus nerve plays a crucial role in the brain-gut-microbiota axis.					
32518376	6	56	theme	SDV + LPS	988:996	arg1	group					998:1002	the SDV + LPS group	984:1002	the SDV + LPS group	984:1002	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	1	57	dep	brain	231:235	arg1	the					227:229	the	227:229	the	227:229	The vagus nerve plays a role in the cross talk between the brain and gut microbiota, which could be involved in depression.					
32518376	1	57	dep	brain	231:235	arg1	microbiota					245:254	microbiota	245:254	microbiota	245:254	The vagus nerve plays a role in the cross talk between the brain and gut microbiota, which could be involved in depression.					
32518376	4	58	theme	medial	736:741	arg1	mPFC					762:765	mPFC	762:765	mPFC	762:765	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	4	58	theme	medial	736:741	arg1	cortex					754:759	the medial prefrontal cortex	732:759	the medial prefrontal cortex (mPFC) in the sham-operated mice	732:792	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	8	59	theme	significant	1271:1281	arg1	alterations					1283:1293	significant alterations	1271:1293	significant alterations	1271:1293	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	3	60	theme	subdiaphragmatic	441:456	arg1	SDV					468:470	SDV	468:470	SDV	468:470	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	3	60	theme	subdiaphragmatic	441:456	arg1	vagotomy					458:465	subdiaphragmatic vagotomy	441:465	subdiaphragmatic vagotomy (SDV)	441:471	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	5	61	theme	depression-like	830:844	arg1	phenotype					846:854	a depression-like phenotype	828:854	a depression-like phenotype	828:854	In contrast, LPS did not produce a depression-like phenotype and downregulated synaptic proteins in the mPFC after SDV.					
32518376	9	62	theme	other	1500:1504	arg1	groups					1512:1517	the other three groups	1496:1517	the other three groups	1496:1517	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
32518376	6	63	theme	pro-inflammatory	954:969	arg1	cytokines					971:979	pro-inflammatory cytokines	954:979	pro-inflammatory cytokines	954:979	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	8	64	theme	microbiota	1302:1311	arg1	diversity					1313:1321	gut microbiota diversity	1298:1321	gut microbiota diversity in sham-operated mice	1298:1343	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	0	65	theme	abnormal	84:91	arg1	composition					93:103	abnormal composition	84:103	abnormal composition	84:103	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	3	66	theme	vagotomy	458:465	arg1	effects					430:436	the effects	426:436	the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype	426:504	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	3	66	theme	vagotomy	458:465	arg1	composition					523:533	the abnormal composition	510:533	the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration	510:605	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	7	67	theme	spleen	1159:1164	arg1	weight					1166:1171	spleen weight	1159:1171	spleen weight	1159:1171	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	6	68	theme	sham + LPS	1033:1042	arg1	group					1044:1048	the sham + LPS group	1029:1048	the sham + LPS group	1029:1048	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	4	69	theme	prefrontal	743:752	arg1	mPFC					762:765	mPFC	762:765	mPFC	762:765	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	4	69	theme	prefrontal	743:752	arg1	cortex					754:759	the medial prefrontal cortex	732:759	the medial prefrontal cortex (mPFC) in the sham-operated mice	732:792	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	0	70	theme	key	2:4	arg1	role					6:9	A key role	0:9	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.	0:170	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	0	71	dep	phenotype	70:78	arg1	the					50:52	the	50:52	the	50:52	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	2	72	theme	modulatory	347:356	arg1	nerve					323:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	2	72	theme	modulatory	347:356	arg1	pathway					358:364	a major modulatory pathway	339:364	a major modulatory pathway between the brain and gut microbiota	339:401	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	5	73	from	proteins	883:890	arg1	mPFC					899:902	the mPFC	895:902	the mPFC	895:902	In contrast, LPS did not produce a depression-like phenotype and downregulated synaptic proteins in the mPFC after SDV.					
32518376	8	74	theme	SDV-operated	1354:1365	arg1	mice					1367:1370	SDV-operated mice	1354:1370	SDV-operated mice	1354:1370	Furthermore, LPS led to significant alterations in gut microbiota diversity in sham-operated mice, but not SDV-operated mice.					
32518376	7	75	theme	plasma	1111:1116	arg1	levels					1118:1123	the plasma levels	1107:1123	the plasma levels of pro-inflammatory cytokines and spleen weight	1107:1171	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	6	76	from	weight	926:931	arg1	group					998:1002	the SDV + LPS group	984:1002	the SDV + LPS group	984:1002	The spleen weight and plasma levels of pro-inflammatory cytokines in the SDV + LPS group were lower than those of the sham + LPS group.					
32518376	2	77	dep	brain	378:382	arg1	the					374:376	the	374:376	the	374:376	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	2	77	dep	brain	378:382	arg1	microbiota					392:401	microbiota	392:401	microbiota	392:401	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	10	78	theme	synaptic	1649:1656	arg1	proteins					1658:1665	synaptic proteins	1649:1665	synaptic proteins in the mPFC	1649:1677	Our results suggest that LPS produces a depression-like phenotype, increases spleen weight, triggers inflammation, downregulates synaptic proteins in the mPFC, and leads to abnormal composition of gut microbiota via the subdiaphragmatic vagus nerve.					
32518376	0	79	theme	nerve	41:45	arg1	role					6:9	A key role	0:9	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.	0:170	A key role of the subdiaphragmatic vagus nerve in the depression-like phenotype and abnormal composition of gut microbiota in mice after lipopolysaccharide administration.					
32518376	3	80	from	effects	430:436	arg1	phenotype					496:504	the depression-like phenotype	476:504	the depression-like phenotype	476:504	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	3	80	from	effects	430:436	arg1	mice					556:559	mice	556:559	mice after lipopolysaccharide (LPS) administration	556:605	Here, we investigated the effects of subdiaphragmatic vagotomy (SDV) on the depression-like phenotype and the abnormal composition of gut microbiota in mice after lipopolysaccharide (LPS) administration.					
32518376	2	81	theme	vagus	317:321	arg1	nerve					323:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve	296:327	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	2	81	theme	vagus	317:321	arg1	pathway					358:364	a major modulatory pathway	339:364	a major modulatory pathway between the brain and gut microbiota	339:401	The subdiaphragmatic vagus nerve serves as a major modulatory pathway between the brain and gut microbiota.					
32518376	4	82	from	downregulation	693:706	arg1	weight					681:686	spleen weight	674:686	spleen weight	674:686	LPS caused a depression-like phenotype, inflammation, increase in spleen weight, and downregulation of synaptic proteins in the medial prefrontal cortex (mPFC) in the sham-operated mice.					
32518376	11	83	theme	crucial	1812:1818	arg1	role					1820:1823	a crucial role	1810:1823	a crucial role	1810:1823	It is likely that the vagus nerve plays a crucial role in the brain-gut-microbiota axis.					
32518376	7	84	theme	spleen	1232:1237	arg1	weight					1239:1244	spleen weight	1232:1244	spleen weight	1232:1244	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	7	85	theme	cytokines	1145:1153	arg1	levels					1118:1123	the plasma levels	1107:1123	the plasma levels of pro-inflammatory cytokines and spleen weight	1107:1171	Interestingly, there were positive correlations between the plasma levels of pro-inflammatory cytokines and spleen weight, suggesting a relationship between inflammatory events and spleen weight.					
32518376	9	86	theme	unweighted	1379:1388	arg1	PCoA					1398:1401	an unweighted UniFrac PCoA	1376:1401	an unweighted UniFrac PCoA	1376:1401	In an unweighted UniFrac PCoA, the dots representing the sham + LPS group were located far away from the dots representing the other three groups.					
35026570	2	0	theme	Cu-POM	452:457	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	0	theme	Cu-POM	452:457	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	0	theme	Cu-POM	452:457	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	0	theme	Cu-POM	452:457	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	3	1	dep	strains	704:710	arg1	aureus					727:732	Staphylococcus aureus	712:732	Staphylococcus aureus (S. aureus, 99.98%)	712:752	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	1	dep	strains	704:710	arg1	coli					767:770	Escherichia coli	755:770	Escherichia coli (E. coli, 99.99%)	755:788	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	1	dep	strains	704:710	arg1	strains					828:834	drug-resistant E. coli bacterial strains	795:834	drug-resistant E. coli bacterial strains	795:834	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	6	2	theme	methylene	1289:1297	arg1	removal					1278:1284	removal	1278:1284	removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%)	1278:1428	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	8	3	theme	umbilical	1612:1620	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells	1606:1643	More importantly, the GO/CS/Cu-POM nanocomposite indicated good biocompatibility to human umbilical vein endothelial cells.					
35026570	3	4	dep	aureus	727:732	arg1	aureus					738:743	S. aureus	735:743	S. aureus	735:743	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	4	dep	aureus	727:732	arg1	%					870:870	kanamycin-resistant E. coli 99.93%	837:870	kanamycin-resistant E. coli 99.93%	837:870	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	4	dep	aureus	727:732	arg1	coli					900:903	E. coli	897:903	ampicillin-resistant E. coli	876:903	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	4	dep	aureus	727:732	arg1	%					911:911	97.94%	906:911	97.94%	906:911	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	4	dep	aureus	727:732	arg1	%					751:751	99.98%	746:751	99.98%	746:751	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	8	5	theme	endothelial	1627:1637	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells	1606:1643	More importantly, the GO/CS/Cu-POM nanocomposite indicated good biocompatibility to human umbilical vein endothelial cells.					
35026570	2	6	theme	polyoxometalate	406:420	arg1	nanocomposite					437:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite	351:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole)	351:522	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	6	7	theme	violet	1326:1331	arg1	removal					1278:1284	removal	1278:1284	removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%)	1278:1428	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	5	8	theme	bacterial	1060:1068	arg1	membrane					1070:1077	bacterial membrane	1060:1077	bacterial membrane	1060:1077	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	2	9	theme	graphene	369:376	arg1	nanocomposite					437:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite	351:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole)	351:522	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	1	10	theme	water	268:272	arg1	technologies					284:295	efficient and eco-friendly water treatment technologies	241:295	efficient and eco-friendly water treatment technologies	241:295	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	6	11	theme	superior	1253:1260	arg1	performance					1262:1272	superior performance	1253:1272	superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%)	1253:1428	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	1	12	theme	technologies	284:295	arg1	development					226:236	the development	222:236	the development of efficient and eco-friendly water treatment technologies and materials	222:309	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	1	12	theme	technologies	284:295	arg1	desirable					321:329	desirable	321:329	desirable	321:329	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	2	13	theme	ultrasound-assisted	547:565	arg1	strategy					581:588	the ultrasound-assisted self-assembly strategy	543:588	the ultrasound-assisted self-assembly strategy	543:588	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	3	14	dep	E.	810:811	arg1	coli					813:816	coli	813:816	coli	813:816	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	1	15	theme	drug-resistant	168:181	arg1	pathogens					183:191	drug-resistant pathogens	168:191	drug-resistant pathogens	168:191	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	9	16	theme	Current	1646:1652	arg1	work					1654:1657	Current work	1646:1657	Current work	1646:1657	Current work provides an effective strategy to design multifunctional POM-based composites for water purification and environmental protection.					
35026570	6	17	theme	GO/CS/Cu-POM	1211:1222	arg1	nanocomposite					1224:1236	the GO/CS/Cu-POM nanocomposite	1207:1236	the GO/CS/Cu-POM nanocomposite	1207:1236	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	3	18	theme	potent	632:637	arg1	properties					652:661	potent bactericidal properties	632:661	potent bactericidal properties	632:661	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	6	19	theme	basic	1347:1351	arg1	fuchsin					1353:1359	basic fuchsin	1347:1359	basic fuchsin (BF, 96.47%)	1347:1372	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	1	20	theme	contaminants	208:219	arg1	rise					160:163	the rise	156:163	the rise of drug-resistant pathogens and industrial contaminants	156:219	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	7	21	theme	dye	1461:1463	arg1	removal					1465:1471	dye removal	1461:1471	dye removal	1461:1471	Moreover, the main process of dye removal by GO/CS/Cu-POM was controlled by chemisorption.					
35026570	9	22	theme	effective	1671:1679	arg1	strategy					1681:1688	an effective strategy	1668:1688	an effective strategy to design multifunctional POM-based composites for water purification and environmental protection	1668:1787	Current work provides an effective strategy to design multifunctional POM-based composites for water purification and environmental protection.					
35026570	3	23	theme	bacterial	818:826	arg1	strains					828:834	drug-resistant E. coli bacterial strains	795:834	drug-resistant E. coli bacterial strains	795:834	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	0	24	theme	oxide	128:132	arg1	nanocomposites					134:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	Ultrahigh-efficiency antibacterial and adsorption performance induced by copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites.					
35026570	3	25	theme	E.	810:811	arg1	strains					828:834	drug-resistant E. coli bacterial strains	795:834	drug-resistant E. coli bacterial strains	795:834	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	26	dep	E.	857:858	arg1	coli					860:863	coli	860:863	coli	860:863	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	2	27	theme	[P2Mo5O23	493:501	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	27	theme	[P2Mo5O23	493:501	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	27	theme	[P2Mo5O23	493:501	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	27	theme	[P2Mo5O23	493:501	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	5	28	theme	oxidative	1098:1106	arg1	stress					1108:1113	oxidative stress	1098:1113	oxidative stress induced by GO/CS/Cu-POM	1098:1137	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	0	29	theme	graphene	119:126	arg1	nanocomposites					134:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	Ultrahigh-efficiency antibacterial and adsorption performance induced by copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites.					
35026570	0	30	theme	Ultrahigh-efficiency	0:19	arg1	antibacterial					21:33	Ultrahigh-efficiency antibacterial	0:33	Ultrahigh-efficiency antibacterial	0:33	Ultrahigh-efficiency antibacterial and adsorption performance induced by copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites.					
35026570	4	31	from	GO/CS/Cu-POM	1027:1038	arg1	performance					942:952	the antibacterial performance	924:952	the antibacterial performance	924:952	Further, the antibacterial performance was strongly dependent on synergistic effect between GO/CS and Cu-POM in GO/CS/Cu-POM.					
35026570	4	31	from	GO/CS/Cu-POM	1027:1038	arg1	dependent					967:975	dependent	967:975	dependent	967:975	Further, the antibacterial performance was strongly dependent on synergistic effect between GO/CS and Cu-POM in GO/CS/Cu-POM.					
35026570	9	32	theme	water	1741:1745	arg1	purification					1747:1758	water purification	1741:1758	water purification	1741:1758	Current work provides an effective strategy to design multifunctional POM-based composites for water purification and environmental protection.					
35026570	3	33	theme	gram-positive/negative	671:692	arg1	strains					704:710	gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%)	671:912	gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%)	671:912	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	9	34	theme	environmental	1764:1776	arg1	protection					1778:1787	environmental protection	1764:1787	environmental protection	1764:1787	Current work provides an effective strategy to design multifunctional POM-based composites for water purification and environmental protection.					
35026570	2	35	theme	[Cu	481:483	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	35	theme	[Cu	481:483	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	35	theme	[Cu	481:483	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	35	theme	[Cu	481:483	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	0	36	theme	polyoxomolybdate-decorated	92:117	arg1	nanocomposites					134:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	Ultrahigh-efficiency antibacterial and adsorption performance induced by copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites.					
35026570	9	37	theme	POM-based	1716:1724	arg1	composites					1726:1735	multifunctional POM-based composites	1700:1735	multifunctional POM-based composites for water purification and environmental protection	1700:1787	Current work provides an effective strategy to design multifunctional POM-based composites for water purification and environmental protection.					
35026570	8	38	theme	GO/CS/Cu-POM	1544:1555	arg1	nanocomposite					1557:1569	the GO/CS/Cu-POM nanocomposite	1540:1569	the GO/CS/Cu-POM nanocomposite	1540:1569	More importantly, the GO/CS/Cu-POM nanocomposite indicated good biocompatibility to human umbilical vein endothelial cells.					
35026570	6	39	dep	NC	1418:1419	arg1	%					1427:1427	76.26%	1422:1427	76.26%	1422:1427	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	6	40	theme	blue	1299:1302	arg1	methylene					1289:1297	methylene blue	1289:1302	methylene blue (MB, 96.86%)	1289:1315	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	2	41	theme	multifunctional	353:367	arg1	nanocomposite					437:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite	351:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole)	351:522	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	6	42	dep	methylene	1289:1297	arg1	%					1314:1314	96.86%	1309:1314	96.86%	1309:1314	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	6	42	dep	methylene	1289:1297	arg1	MB					1305:1306	MB	1305:1306	MB	1305:1306	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	2	43	theme	self-assembly	567:579	arg1	strategy					581:588	the ultrasound-assisted self-assembly strategy	543:588	the ultrasound-assisted self-assembly strategy	543:588	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	8	44	theme	human	1606:1610	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells	1606:1643	More importantly, the GO/CS/Cu-POM nanocomposite indicated good biocompatibility to human umbilical vein endothelial cells.					
35026570	8	45	theme	vein	1622:1625	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells	1606:1643	More importantly, the GO/CS/Cu-POM nanocomposite indicated good biocompatibility to human umbilical vein endothelial cells.					
35026570	1	46	theme	efficient	241:249	arg1	technologies					284:295	efficient and eco-friendly water treatment technologies	241:295	efficient and eco-friendly water treatment technologies	241:295	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	2	47	theme	oxide/chitosan/copper-based	378:404	arg1	nanocomposite					437:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite	351:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole)	351:522	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	1	48	theme	eco-friendly	255:266	arg1	technologies					284:295	efficient and eco-friendly water treatment technologies	241:295	efficient and eco-friendly water treatment technologies	241:295	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	6	49	theme	gentian	1318:1324	arg1	violet					1326:1331	gentian violet	1318:1331	gentian violet (GV, 97.77%)	1318:1344	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	5	50	theme	membrane	1070:1077	arg1	destruction					1045:1055	The destruction	1041:1055	The destruction of bacterial membrane	1041:1077	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	5	50	theme	membrane	1070:1077	arg1	levels					1088:1093	high levels	1083:1093	high levels of oxidative stress induced by GO/CS/Cu-POM	1083:1137	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	1	51	theme	treatment	274:282	arg1	technologies					284:295	efficient and eco-friendly water treatment technologies	241:295	efficient and eco-friendly water treatment technologies	241:295	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	5	52	theme	high	1083:1086	arg1	levels					1088:1093	high levels	1083:1093	high levels of oxidative stress induced by GO/CS/Cu-POM	1083:1137	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	6	53	dep	violet	1326:1331	arg1	GV					1334:1335	GV	1334:1335	GV	1334:1335	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	6	53	dep	violet	1326:1331	arg1	%					1343:1343	97.77%	1338:1343	97.77%	1338:1343	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	1	54	theme	pathogens	183:191	arg1	rise					160:163	the rise	156:163	the rise of drug-resistant pathogens and industrial contaminants	156:219	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	7	55	theme	removal	1465:1471	arg1	process					1450:1456	the main process	1441:1456	the main process of dye removal by GO/CS/Cu-POM	1441:1487	Moreover, the main process of dye removal by GO/CS/Cu-POM was controlled by chemisorption.					
35026570	3	56	theme	drug-resistant	795:808	arg1	strains					828:834	drug-resistant E. coli bacterial strains	795:834	drug-resistant E. coli bacterial strains	795:834	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	6	57	theme	fuchsin	1353:1359	arg1	removal					1278:1284	removal	1278:1284	removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%)	1278:1428	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	3	58	theme	GO/CS/Cu-POM	595:606	arg1	nanocomposite					608:620	The GO/CS/Cu-POM nanocomposite	591:620	The GO/CS/Cu-POM nanocomposite	591:620	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	1	59	theme	industrial	197:206	arg1	contaminants					208:219	industrial contaminants	197:219	industrial contaminants	197:219	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	3	60	theme	kanamycin-resistant	837:855	arg1	%					870:870	kanamycin-resistant E. coli 99.93%	837:870	kanamycin-resistant E. coli 99.93%	837:870	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	60	theme	kanamycin-resistant	837:855	arg1	%					911:911	97.94%	906:911	97.94%	906:911	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	61	dep	coli	767:770	arg1	E.					773:774	E.	773:774	E.	773:774	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	61	dep	coli	767:770	arg1	%					787:787	99.99%	782:787	99.99%	782:787	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	7	62	theme	main	1445:1448	arg1	process					1450:1456	the main process	1441:1456	the main process of dye removal by GO/CS/Cu-POM	1441:1487	Moreover, the main process of dye removal by GO/CS/Cu-POM was controlled by chemisorption.					
35026570	3	63	dep	E.	773:774	arg1	coli					776:779	E. coli	773:779	E. coli	773:779	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	3	64	dep	kanamycin-resistant	837:855	arg1	E.					857:858	E.	857:858	E.	857:858	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	4	65	theme	antibacterial	928:940	arg1	performance					942:952	the antibacterial performance	924:952	the antibacterial performance	924:952	Further, the antibacterial performance was strongly dependent on synergistic effect between GO/CS and Cu-POM in GO/CS/Cu-POM.					
35026570	4	65	theme	antibacterial	928:940	arg1	dependent					967:975	dependent	967:975	dependent	967:975	Further, the antibacterial performance was strongly dependent on synergistic effect between GO/CS and Cu-POM in GO/CS/Cu-POM.					
35026570	1	66	theme	materials	301:309	arg1	development					226:236	the development	222:236	the development of efficient and eco-friendly water treatment technologies and materials	222:309	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	1	66	theme	materials	301:309	arg1	desirable					321:329	desirable	321:329	desirable	321:329	Given the rise of drug-resistant pathogens and industrial contaminants, the development of efficient and eco-friendly water treatment technologies and materials is highly desirable and urgent.					
35026570	9	67	theme	multifunctional	1700:1714	arg1	composites					1726:1735	multifunctional POM-based composites	1700:1735	multifunctional POM-based composites for water purification and environmental protection	1700:1787	Current work provides an effective strategy to design multifunctional POM-based composites for water purification and environmental protection.					
35026570	6	68	theme	tetracycline	1375:1386	arg1	removal					1278:1284	removal	1278:1284	removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%)	1278:1428	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	5	69	theme	stress	1108:1113	arg1	destruction					1045:1055	The destruction	1041:1055	The destruction of bacterial membrane	1041:1077	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	5	69	theme	stress	1108:1113	arg1	levels					1088:1093	high levels	1083:1093	high levels of oxidative stress induced by GO/CS/Cu-POM	1083:1137	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	6	70	dep	fuchsin	1353:1359	arg1	BF					1362:1363	BF	1362:1363	BF	1362:1363	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	6	70	dep	fuchsin	1353:1359	arg1	%					1371:1371	96.47%	1366:1371	96.47%	1366:1371	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	2	71	theme	L	464:464	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	71	theme	L	464:464	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	71	theme	L	464:464	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	71	theme	L	464:464	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	0	72	theme	adsorption	39:48	arg1	performance					50:60	adsorption performance	39:60	adsorption performance	39:60	Ultrahigh-efficiency antibacterial and adsorption performance induced by copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites.					
35026570	5	73	theme	bactericidal	1172:1183	arg1	process					1185:1191	the bactericidal process	1168:1191	the bactericidal process	1168:1191	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	2	74	dep	nanocomposite	437:449	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	74	dep	nanocomposite	437:449	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	74	dep	nanocomposite	437:449	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	74	dep	nanocomposite	437:449	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	75	theme	L	472:472	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	75	theme	L	472:472	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	75	theme	L	472:472	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	75	theme	L	472:472	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	76	dep	·4H2O	503:507	arg1	[Cu					460:462	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	[Cu	460:462	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	4	77	theme	synergistic	980:990	arg1	effect					992:997	synergistic effect	980:997	synergistic effect	980:997	Further, the antibacterial performance was strongly dependent on synergistic effect between GO/CS and Cu-POM in GO/CS/Cu-POM.					
35026570	3	78	theme	bactericidal	639:650	arg1	properties					652:661	potent bactericidal properties	632:661	potent bactericidal properties	632:661	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	2	79	theme	[Cu	468:470	arg1	H2O					488:490	H2O	488:490	H2O	488:490	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	79	theme	[Cu	468:470	arg1	H2O					476:478	H2O	476:478	H2O	476:478	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	79	theme	[Cu	468:470	arg1	L = pyrazole					510:521	L = pyrazole	510:521	L = pyrazole	510:521	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	2	79	theme	[Cu	468:470	arg1	·4H2O					503:507	Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O	452:507	·4H2O	503:507	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	0	80	theme	copper-substituted	73:90	arg1	nanocomposites					134:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites	73:147	Ultrahigh-efficiency antibacterial and adsorption performance induced by copper-substituted polyoxomolybdate-decorated graphene oxide nanocomposites.					
35026570	5	81	theme	significant	1148:1158	arg1	role					1160:1163	a significant role	1146:1163	a significant role	1146:1163	The destruction of bacterial membrane and high levels of oxidative stress induced by GO/CS/Cu-POM played a significant role in the bactericidal process.					
35026570	6	82	theme	norfloxacin	1405:1415	arg1	removal					1278:1284	removal	1278:1284	removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%)	1278:1428	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	6	83	dep	tetracycline	1375:1386	arg1	TC					1389:1390	TC	1389:1390	TC	1389:1390	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	6	83	dep	tetracycline	1375:1386	arg1	%					1398:1398	78.92%	1393:1398	78.92%	1393:1398	Furthermore, the GO/CS/Cu-POM nanocomposite also displayed superior performance for removal of methylene blue (MB, 96.86%), gentian violet (GV, 97.77%), basic fuchsin (BF, 96.47%), tetracycline (TC, 78.92%) and norfloxacin (NC, 76.26%).					
35026570	3	84	theme	bacterial	694:702	arg1	strains					704:710	gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%)	671:912	gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%)	671:912	The GO/CS/Cu-POM nanocomposite exhibited potent bactericidal properties against gram-positive/negative bacterial strains Staphylococcus aureus (S. aureus, 99.98%), Escherichia coli (E. coli, 99.99%), and drug-resistant E. coli bacterial strains (kanamycin-resistant E. coli 99.93% and ampicillin-resistant E. coli, 97.94%).					
35026570	2	85	theme	GO/CS/Cu-POM	423:434	arg1	nanocomposite					437:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite	351:449	a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole)	351:522	Herein, a multifunctional graphene oxide/chitosan/copper-based polyoxometalate (GO/CS/Cu-POM) nanocomposite (Cu-POM, [Cu(L)4][Cu(L)3(H2O)][Cu(L)(H2O)][P2Mo5O23]·4H2O, L = pyrazole) was synthesized by the ultrasound-assisted self-assembly strategy.					
35026570	4	86	from	dependent	967:975	arg1	GO/CS/Cu-POM					1027:1038	GO/CS/Cu-POM	1027:1038	GO/CS/Cu-POM	1027:1038	Further, the antibacterial performance was strongly dependent on synergistic effect between GO/CS and Cu-POM in GO/CS/Cu-POM.					
35026570	8	87	theme	good	1581:1584	arg1	biocompatibility					1586:1601	good biocompatibility	1581:1601	good biocompatibility to human umbilical vein endothelial cells	1581:1643	More importantly, the GO/CS/Cu-POM nanocomposite indicated good biocompatibility to human umbilical vein endothelial cells.					
33878359	2	0	theme	carboxymethyl	446:458	arg1	cellulose					460:468	carboxymethyl cellulose	446:468	carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier	446:525	The subject of this work is to synthesis a novel carrier system through coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier.					
33878359	2	0	theme	carboxymethyl	446:458	arg1	CMC					471:473	CMC	471:473	CMC	471:473	The subject of this work is to synthesis a novel carrier system through coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier.					
33878359	1	1	theme	different	316:324	arg1	areas					337:341	different biomedical areas	316:341	different biomedical areas	316:341	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	4	2	theme	carrier	767:773	arg1	immersion					754:762	immersion	754:762	immersion of carrier to the TC aqueous solution	754:800	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	1	3	with	carrier	221:227	arg1	profile					261:267	a highly prolonged release profile	234:267	a highly prolonged release profile	234:267	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	7	4	with	CMC/MIL-88	1173:1182	arg1	mg·ml-1					1234:1240	MIC 64 mg·ml-1	1227:1240	MIC 64 mg·ml-1	1227:1240	The antibacterial activity was found for TC-loaded CMC/MIL-88(Fe) toward both E. coli and S. aureus with MIC 64 mg·ml-1.					
33878359	4	5	theme	aqueous	785:791	arg1	solution					793:800	the TC aqueous solution	778:800	the TC aqueous solution	778:800	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	0	6	theme	cellulose	73:81	arg1	fibers					83:88	carboxymethyl cellulose fibers	59:88	carboxymethyl cellulose fibers	59:88	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	1	7	theme	biomedical	326:335	arg1	areas					337:341	different biomedical areas	316:341	different biomedical areas	316:341	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	1	8	theme	prolonged	243:251	arg1	profile					261:267	a highly prolonged release profile	234:267	a highly prolonged release profile	234:267	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	6	9	theme	HFF-1	1044:1048	arg1	cells					1051:1055	Human skin fibroblast (HFF-1) cells	1021:1055	Human skin fibroblast (HFF-1) cells	1021:1055	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	1	10	theme	release	253:259	arg1	profile					261:267	a highly prolonged release profile	234:267	a highly prolonged release profile	234:267	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	3	11	theme	Fe	666:667	arg1	composite					670:678	CMC/MIL-88(Fe) composite	655:678	CMC/MIL-88(Fe) composite	655:678	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	3	12	dep	in	587:588	arg1	situ					590:593	situ	590:593	situ	590:593	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	6	13	theme	fibroblast	1032:1041	arg1	cells					1051:1055	Human skin fibroblast (HFF-1) cells	1021:1055	Human skin fibroblast (HFF-1) cells	1021:1055	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	2	14	theme	work	364:367	arg1	subject					348:354	The subject	344:354	The subject of this work	344:367	The subject of this work is to synthesis a novel carrier system through coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier.					
33878359	0	15	theme	safe	93:96	arg1	carrier					98:104	A safe carrier	91:104	A safe carrier for highly sustained release of tetracycline	91:149	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	3	16	theme	CMC/MIL-88	655:664	arg1	composite					670:678	CMC/MIL-88(Fe) composite	655:678	CMC/MIL-88(Fe) composite	655:678	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	6	17	theme	good	1098:1101	arg1	cytocompatibility					1103:1119	a good cytocompatibility	1096:1119	a good cytocompatibility	1096:1119	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	5	18	from	profile	815:821	arg1	conditions					854:863	the simulated physiological conditions	826:863	the simulated physiological conditions	826:863	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	6	19	theme	skin	1027:1030	arg1	cells					1051:1055	Human skin fibroblast (HFF-1) cells	1021:1055	Human skin fibroblast (HFF-1) cells	1021:1055	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	1	20	theme	long-time	165:173	arg1	infection					193:201	long-time harmful bacterial infection	165:201	long-time harmful bacterial infection	165:201	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	7	21	theme	TC-loaded	1163:1171	arg1	Fe					1184:1185	Fe	1184:1185	Fe	1184:1185	The antibacterial activity was found for TC-loaded CMC/MIL-88(Fe) toward both E. coli and S. aureus with MIC 64 mg·ml-1.					
33878359	7	21	theme	TC-loaded	1163:1171	arg1	CMC/MIL-88					1173:1182	TC-loaded CMC/MIL-88	1163:1182	TC-loaded CMC/MIL-88(Fe) toward both E. coli and S. aureus with MIC 64 mg·ml-1	1163:1240	The antibacterial activity was found for TC-loaded CMC/MIL-88(Fe) toward both E. coli and S. aureus with MIC 64 mg·ml-1.					
33878359	5	22	theme	low	885:887	arg1	release					903:909	a low initial burst release	883:909	a low initial burst release followed by a sustained and prolonged release over 384 h	883:966	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	6	23	theme	Human	1021:1025	arg1	cells					1051:1055	Human skin fibroblast (HFF-1) cells	1021:1055	Human skin fibroblast (HFF-1) cells	1021:1055	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	1	24	theme	harmful	175:181	arg1	infection					193:201	long-time harmful bacterial infection	165:201	long-time harmful bacterial infection	165:201	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	0	25	theme	Ultrasound-assisted	0:18	arg1	synthesis					20:28	Ultrasound-assisted synthesis	0:28	Ultrasound-assisted synthesis of MIL-88(Fe)	0:42	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	5	26	theme	simulated	830:838	arg1	conditions					854:863	the simulated physiological conditions	826:863	the simulated physiological conditions	826:863	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	1	27	theme	bacterial	183:191	arg1	infection					193:201	long-time harmful bacterial infection	165:201	long-time harmful bacterial infection	165:201	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	0	28	theme	MIL-88	33:38	arg1	synthesis					20:28	Ultrasound-assisted synthesis	0:28	Ultrasound-assisted synthesis of MIL-88(Fe)	0:42	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	3	29	dep	CMC	638:640	arg1	the					622:624	the	622:624	the	622:624	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	3	29	dep	CMC	638:640	arg1	presence					626:633	presence	626:633	presence	626:633	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	4	30	theme	Tetracycline	733:744	arg1	TC					747:748	TC	747:748	TC	747:748	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	4	30	theme	Tetracycline	733:744	arg1	Tetracycline					733:744	Tetracycline	733:744	Tetracycline (TC)	733:749	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	4	30	theme	Tetracycline	733:744	arg1	amount					723:728	a high amount	716:728	a high amount of Tetracycline (TC)	716:749	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	5	31	theme	physiological	840:852	arg1	conditions					854:863	the simulated physiological conditions	826:863	the simulated physiological conditions	826:863	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	5	32	theme	burst	897:901	arg1	release					903:909	a low initial burst release	883:909	a low initial burst release followed by a sustained and prolonged release over 384 h	883:966	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	2	33	theme	carrier	393:399	arg1	system					401:406	a novel carrier system	385:406	a novel carrier system	385:406	The subject of this work is to synthesis a novel carrier system through coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier.					
33878359	1	34	theme	promising	274:282	arg1	approach					284:291	a promising approach	272:291	a promising approach that is of interest to different biomedical areas	272:341	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	1	34	theme	promising	274:282	arg1	carrier					221:227	a drug carrier	214:227	designing a drug carrier with a highly prolonged release profile	204:267	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	2	35	theme	novel	387:391	arg1	system					401:406	a novel carrier system	385:406	a novel carrier system	385:406	The subject of this work is to synthesis a novel carrier system through coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier.					
33878359	6	36	dep	in	973:974	arg1	vitro					976:980	vitro	976:980	vitro	976:980	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	6	37	theme	CMC/MIL-88	998:1007	arg1	cytotoxicity					982:993	The in vitro cytotoxicity	969:993	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells	969:1055	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	3	38	theme	synthetic	566:574	arg1	method					576:581	an ultrasound-assisted synthetic method	543:581	an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe)	543:617	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	1	39	theme	designing	204:212	arg1	approach					284:291	a promising approach	272:291	a promising approach that is of interest to different biomedical areas	272:341	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	1	39	theme	designing	204:212	arg1	carrier					221:227	a drug carrier	214:227	designing a drug carrier with a highly prolonged release profile	204:267	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	0	40	theme	sustained	117:125	arg1	release					127:133	highly sustained release	110:133	highly sustained release of tetracycline	110:149	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	0	41	theme	tetracycline	138:149	arg1	release					127:133	highly sustained release	110:133	highly sustained release of tetracycline	110:149	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	3	42	theme	MIL-88	608:613	arg1	synthesis					595:603	in situ synthesis	587:603	in situ synthesis of MIL-88(Fe)	587:617	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	6	43	theme	MTT	1075:1077	arg1	assay					1079:1083	MTT assay	1075:1083	MTT assay	1075:1083	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33878359	7	44	theme	antibacterial	1126:1138	arg1	activity					1140:1147	The antibacterial activity	1122:1147	The antibacterial activity	1122:1147	The antibacterial activity was found for TC-loaded CMC/MIL-88(Fe) toward both E. coli and S. aureus with MIC 64 mg·ml-1.					
33878359	2	45	theme	MIL-88	432:437	arg1	coordination					416:427	coordination	416:427	coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier	416:525	The subject of this work is to synthesis a novel carrier system through coordination of MIL-88(Fe) to carboxymethyl cellulose (CMC) for enhancing interaction between drug and carrier.					
33878359	4	46	theme	TC	782:783	arg1	solution					793:800	the TC aqueous solution	778:800	the TC aqueous solution	778:800	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	3	47	theme	ultrasound-assisted	546:564	arg1	method					576:581	an ultrasound-assisted synthetic method	543:581	an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe)	543:617	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	5	48	theme	release	807:813	arg1	pH					866:867	pH 7.4	866:871	pH 7.4	866:871	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	5	48	theme	release	807:813	arg1	profile					815:821	The release profile	803:821	The release profile in the simulated physiological conditions	803:863	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	5	49	theme	initial	889:895	arg1	release					903:909	a low initial burst release	883:909	a low initial burst release followed by a sustained and prolonged release over 384 h	883:966	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	5	50	theme	sustained	925:933	arg1	release					949:955	a sustained and prolonged release	923:955	a sustained and prolonged release over 384 h	923:966	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	7	51	theme	MIC	1227:1229	arg1	mg·ml-1					1234:1240	MIC 64 mg·ml-1	1227:1240	MIC 64 mg·ml-1	1227:1240	The antibacterial activity was found for TC-loaded CMC/MIL-88(Fe) toward both E. coli and S. aureus with MIC 64 mg·ml-1.					
33878359	1	52	theme	drug	216:219	arg1	approach					284:291	a promising approach	272:291	a promising approach that is of interest to different biomedical areas	272:341	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	1	52	theme	drug	216:219	arg1	carrier					221:227	a drug carrier	214:227	designing a drug carrier with a highly prolonged release profile	204:267	For stopping long-time harmful bacterial infection, designing a drug carrier with a highly prolonged release profile is a promising approach that is of interest to different biomedical areas.					
33878359	0	53	theme	carboxymethyl	59:71	arg1	fibers					83:88	carboxymethyl cellulose fibers	59:88	carboxymethyl cellulose fibers	59:88	Ultrasound-assisted synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers: A safe carrier for highly sustained release of tetracycline.					
33878359	5	54	theme	prolonged	939:947	arg1	release					949:955	a sustained and prolonged release	923:955	a sustained and prolonged release over 384 h	923:966	The release profile in the simulated physiological conditions, pH 7.4, revealed a low initial burst release followed by a sustained and prolonged release over 384 h.					
33878359	4	55	theme	high	718:721	arg1	TC					747:748	TC	747:748	TC	747:748	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	4	55	theme	high	718:721	arg1	Tetracycline					733:744	Tetracycline	733:744	Tetracycline (TC)	733:749	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	4	55	theme	high	718:721	arg1	amount					723:728	a high amount	716:728	a high amount of Tetracycline (TC)	716:749	The CMC/MIL-88(Fe) was loaded with a high amount of Tetracycline (TC) by immersion of carrier to the TC aqueous solution.					
33878359	3	56	theme	in	587:588	arg1	synthesis					595:603	in situ synthesis	587:603	in situ synthesis of MIL-88(Fe)	587:617	We established an ultrasound-assisted synthetic method for in situ synthesis of MIL-88(Fe) in the presence of CMC resulting in CMC/MIL-88(Fe) composite.					
33878359	6	57	theme	in	973:974	arg1	cytotoxicity					982:993	The in vitro cytotoxicity	969:993	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells	969:1055	The in vitro cytotoxicity of CMC/MIL-88(Fe) against Human skin fibroblast (HFF-1) cells was calculated by MTT assay and showed a good cytocompatibility.					
33838808	4	0	theme	water	684:688	arg1	diffusion					690:698	water diffusion	684:698	water diffusion	684:698	The compact structure of P-CSNPs film prevented water diffusion, reducing the water amount within.					
33838808	2	1	theme	physical	412:419	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	1	2	theme	chitosan	268:275	arg1	P-CSNPs					292:298	P-CSNPs	292:298	P-CSNPs	292:298	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	1	2	theme	chitosan	268:275	arg1	nanoparticles					277:289	p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles	223:289	p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs)	223:299	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	1	3	from	concentrations	314:327	arg1	films					212:216	polyvinyl alcohol/starch composite films	177:216	polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations	177:327	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	6	4	theme	thermal	883:889	arg1	stability					891:899	better thermal stability	876:899	better thermal stability	876:899	Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity.					
33838808	9	5	theme	P-CSNPs	1221:1227	arg1	films					1229:1233	P-CSNPs films	1221:1233	P-CSNPs films	1221:1233	Additionally, P-CS films were less cytotoxic compared to P-CSNPs films.					
33838808	4	6	theme	compact	640:646	arg1	structure					648:656	The compact structure	636:656	The compact structure of P-CSNPs film	636:672	The compact structure of P-CSNPs film prevented water diffusion, reducing the water amount within.					
33838808	5	7	theme	vapor	820:824	arg1	transmittance					826:838	water vapor transmittance	814:838	water vapor transmittance	814:838	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	6	8	theme	better	876:881	arg1	stability					891:899	better thermal stability	876:899	better thermal stability	876:899	Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity.					
33838808	6	9	theme	P-CS	907:910	arg1	films					912:916	P-CS films	907:916	P-CS films	907:916	Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity.					
33838808	5	10	theme	water	814:818	arg1	transmittance					826:838	water vapor transmittance	814:838	water vapor transmittance	814:838	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	3	11	theme	tensile	560:566	arg1	TS					578:579	TS	578:579	TS	578:579	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	3	11	theme	tensile	560:566	arg1	strength					568:575	the tensile strength	556:575	the tensile strength (TS) of the film from 15.67 MPa	556:607	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	2	12	theme	thermal	403:409	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	1	13	theme	polyvinyl	177:185	arg1	films					212:216	polyvinyl alcohol/starch composite films	177:216	polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations	177:327	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	5	14	from	extent	790:795	arg1	solubility					774:783	water solubility	768:783	water solubility	768:783	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	5	15	theme	water	768:772	arg1	solubility					774:783	water solubility	768:783	water solubility	768:783	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	1	16	theme	alcohol/starch	187:200	arg1	films					212:216	polyvinyl alcohol/starch composite films	177:216	polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations	177:327	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	2	17	theme	optical	467:473	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	8	18	theme	higher	1084:1089	arg1	activity					1105:1112	higher antibacterial activity	1084:1112	higher antibacterial activity	1084:1112	P-CSNPs films demonstrated higher antibacterial activity on both Gram-negative and Gram-positive bacteria.					
33838808	0	19	dep	chitosan	104:111	arg1	nanoparticles					126:138	nanoparticles	126:138	nanoparticles	126:138	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	8	20	theme	Gram-negative	1122:1134	arg1	bacteria					1154:1161	both Gram-negative and Gram-positive bacteria	1117:1161	both Gram-negative and Gram-positive bacteria	1117:1161	P-CSNPs films demonstrated higher antibacterial activity on both Gram-negative and Gram-positive bacteria.					
33838808	1	21	theme	composite	202:210	arg1	films					212:216	polyvinyl alcohol/starch composite films	177:216	polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations	177:327	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	0	22	dep	modified	95:102	arg1	study					155:159	A comparative study	141:159	A comparative study	141:159	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	4	23	theme	P-CSNPs	661:667	arg1	film					669:672	P-CSNPs film	661:672	P-CSNPs film	661:672	The compact structure of P-CSNPs film prevented water diffusion, reducing the water amount within.					
33838808	2	24	theme	cytotoxicity	450:461	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	0	25	theme	comparative	143:153	arg1	study					155:159	A comparative study	141:159	A comparative study	141:159	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	2	26	theme	antibacterial	435:447	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	0	27	theme	polyvinyl	20:28	arg1	films					55:59	polyvinyl alcohol/starch composite films	20:59	polyvinyl alcohol/starch composite films incorporated with p-coumaric acid	20:93	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	8	28	theme	antibacterial	1091:1103	arg1	activity					1105:1112	higher antibacterial activity	1084:1112	higher antibacterial activity	1084:1112	P-CSNPs films demonstrated higher antibacterial activity on both Gram-negative and Gram-positive bacteria.					
33838808	6	29	theme	P-CSNPs	855:861	arg1	films					863:867	P-CSNPs films	855:867	P-CSNPs films	855:867	Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity.					
33838808	5	30	theme	swelling	800:807	arg1	transmittance					826:838	water vapor transmittance	814:838	water vapor transmittance	814:838	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	5	30	theme	swelling	800:807	arg1	reduction					755:763	a reduction	753:763	a reduction in water solubility	753:783	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	5	30	theme	swelling	800:807	arg1	extent					790:795	the extent	786:795	the extent of swelling	786:807	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	3	31	from	MPa	605:607	arg1	TS					578:579	TS	578:579	TS	578:579	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	3	31	from	MPa	605:607	arg1	strength					568:575	the tensile strength	556:575	the tensile strength (TS) of the film from 15.67 MPa	556:607	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	1	32	theme	p-coumaric	223:232	arg1	acid					234:237	p-coumaric acid	223:237	p-coumaric acid modified chitosan (P-CS)	223:262	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	0	33	theme	films	55:59	arg1	Characterization					0:15	Characterization	0:15	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid	0:93	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	1	34	theme	different	304:312	arg1	concentrations					314:327	different concentrations	304:327	different concentrations	304:327	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	2	35	theme	antioxidant	422:432	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	1	36	theme	acid	234:237	arg1	P-CS					258:261	P-CS	258:261	P-CS	258:261	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	1	36	theme	acid	234:237	arg1	chitosan					248:255	p-coumaric acid modified chitosan	223:255	p-coumaric acid modified chitosan (P-CS)	223:262	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	0	37	theme	composite	45:53	arg1	films					55:59	polyvinyl alcohol/starch composite films	20:59	polyvinyl alcohol/starch composite films incorporated with p-coumaric acid	20:93	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	3	38	theme	MPa	631:633	arg1	maximum					614:620	a maximum	612:620	a maximum of 24.32 MPa	612:633	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	0	39	theme	alcohol/starch	30:43	arg1	films					55:59	polyvinyl alcohol/starch composite films	20:59	polyvinyl alcohol/starch composite films incorporated with p-coumaric acid	20:93	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	7	40	theme	good	1017:1020	arg1	ultraviolet-barrier					1022:1040	good ultraviolet-barrier	1017:1040	good ultraviolet-barrier	1017:1040	Besides, the P-CS films exhibited excellent transparency and good ultraviolet-barrier at 200-280 nm.					
33838808	1	41	theme	modified	239:246	arg1	P-CS					258:261	P-CS	258:261	P-CS	258:261	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	1	41	theme	modified	239:246	arg1	chitosan					248:255	p-coumaric acid modified chitosan	223:255	p-coumaric acid modified chitosan (P-CS)	223:262	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	4	42	theme	film	669:672	arg1	structure					648:656	The compact structure	636:656	The compact structure of P-CSNPs film	636:672	The compact structure of P-CSNPs film prevented water diffusion, reducing the water amount within.					
33838808	5	43	from	transmittance	826:838	arg1	solubility					774:783	water solubility	768:783	water solubility	768:783	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	2	44	theme	barrier	475:481	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	6	45	theme	higher	927:932	arg1	activity					946:953	higher antioxidant activity	927:953	higher antioxidant activity	927:953	Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity.					
33838808	1	46	with	films	212:216	arg1	P-CSNPs					292:298	P-CSNPs	292:298	P-CSNPs	292:298	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	1	46	with	films	212:216	arg1	nanoparticles					277:289	p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles	223:289	p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs)	223:299	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	5	47	from	reduction	755:763	arg1	solubility					774:783	water solubility	768:783	water solubility	768:783	Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance.					
33838808	8	48	theme	P-CSNPs	1057:1063	arg1	films					1065:1069	P-CSNPs films	1057:1069	P-CSNPs films	1057:1069	P-CSNPs films demonstrated higher antibacterial activity on both Gram-negative and Gram-positive bacteria.					
33838808	9	49	theme	P-CS	1178:1181	arg1	films					1183:1187	P-CS films	1178:1187	P-CS films	1178:1187	Additionally, P-CS films were less cytotoxic compared to P-CSNPs films.					
33838808	1	50	theme	chitosan	248:255	arg1	P-CSNPs					292:298	P-CSNPs	292:298	P-CSNPs	292:298	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	1	50	theme	chitosan	248:255	arg1	nanoparticles					277:289	p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles	223:289	p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs)	223:299	In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared.					
33838808	8	51	theme	Gram-positive	1140:1152	arg1	bacteria					1154:1161	both Gram-negative and Gram-positive bacteria	1117:1161	both Gram-negative and Gram-positive bacteria	1117:1161	P-CSNPs films demonstrated higher antibacterial activity on both Gram-negative and Gram-positive bacteria.					
33838808	4	52	theme	water	714:718	arg1	amount					720:725	the water amount	710:725	the water amount within	710:732	The compact structure of P-CSNPs film prevented water diffusion, reducing the water amount within.					
33838808	7	53	theme	excellent	990:998	arg1	transparency					1000:1011	excellent transparency	990:1011	excellent transparency	990:1011	Besides, the P-CS films exhibited excellent transparency and good ultraviolet-barrier at 200-280 nm.					
33838808	2	54	theme	mechanical	391:400	arg1	properties					483:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties	385:492	The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties.					
33838808	0	55	theme	p-coumaric	79:88	arg1	acid					90:93	p-coumaric acid	79:93	p-coumaric acid	79:93	Characterization of polyvinyl alcohol/starch composite films incorporated with p-coumaric acid modified chitosan and chitosan nanoparticles: A comparative study.					
33838808	7	56	theme	P-CS	969:972	arg1	films					974:978	the P-CS films	965:978	the P-CS films	965:978	Besides, the P-CS films exhibited excellent transparency and good ultraviolet-barrier at 200-280 nm.					
33838808	3	57	theme	film	589:592	arg1	TS					578:579	TS	578:579	TS	578:579	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	3	57	theme	film	589:592	arg1	strength					568:575	the tensile strength	556:575	the tensile strength (TS) of the film from 15.67 MPa	556:607	The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa.					
33838808	6	58	theme	antioxidant	934:944	arg1	activity					946:953	higher antioxidant activity	927:953	higher antioxidant activity	927:953	Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity.					
33845441	5	0	theme	increasing	792:801	arg1	conductivity					818:829	increasing the electrical conductivity	792:829	increasing the electrical conductivity	792:829	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	4	1	theme	graphene	503:510	arg1	substrate					584:592	a biocompatible substrate	568:592	a biocompatible substrate for the receptor stabilization	568:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	4	1	theme	graphene	503:510	arg1	composite					546:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	7	2	theme	aptasensor	1078:1087	arg1	steps					1101:1105	The aptasensor fabrication steps	1074:1105	The aptasensor fabrication steps	1074:1105	The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy.					
33845441	5	3	theme	composite	771:779	arg1	properties					753:762	the properties	749:762	the properties of the composite	749:779	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	9	4	theme	wide	1495:1498	arg1	range					1507:1511	a wide linear range	1493:1511	a wide linear range of 1 × 101-1 × 106 cells/mL	1493:1539	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	5	5	theme	electrical	807:816	arg1	conductivity					818:829	increasing the electrical conductivity	792:829	increasing the electrical conductivity	792:829	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	5	6	theme	significant	630:640	arg1	function					642:649	The significant function	626:649	The significant function of the aptamer on this composite	626:682	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	5	6	theme	significant	630:640	arg1	due					687:689	due	687:689	due	687:689	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	2	7	theme	inexpensive	348:358	arg1	methods					385:391	fast, inexpensive, and selective detection methods	342:391	fast, inexpensive, and selective detection methods	342:391	Therefore, there is a great demand for fast, inexpensive, and selective detection methods.					
33845441	4	8	theme	receptor	602:609	arg1	stabilization					611:623	the receptor stabilization	598:623	the receptor stabilization	598:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	0	9	theme	graphene	92:99	arg1	composite					134:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	4	10	theme	nanoparticles	532:544	arg1	substrate					584:592	a biocompatible substrate	568:592	a biocompatible substrate for the receptor stabilization	568:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	4	10	theme	nanoparticles	532:544	arg1	composite					546:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	8	11	theme	Ferro/Ferricyanide	1297:1314	arg1	access					1316:1321	the Ferro/Ferricyanide access	1293:1321	the Ferro/Ferricyanide access to the sensor	1293:1335	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	0	12	theme	reduced	84:90	arg1	composite					134:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	8	13	theme	charge	1363:1368	arg1	resistances					1379:1389	the charge transfer resistances	1359:1389	the charge transfer resistances	1359:1389	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	1	14	theme	mortality	282:290	arg1	reduction					292:300	mortality reduction	282:300	mortality reduction	282:300	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	0	15	theme	nanoparticle	121:132	arg1	composite					134:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	6	16	theme	membrane	923:930	arg1	proteins					932:939	the cell membrane proteins	914:939	the cell membrane proteins	914:939	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	5	17	theme	aptamer	658:664	arg1	function					642:649	The significant function	626:649	The significant function of the aptamer on this composite	626:682	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	5	17	theme	aptamer	658:664	arg1	due					687:689	due	687:689	due	687:689	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	3	18	theme	cell	477:480	arg1	detection					482:490	sensitive human breast cancer cell detection	447:490	sensitive human breast cancer cell detection	447:490	Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection.					
33845441	1	19	theme	early	186:190	arg1	detection					192:200	early detection	186:200	early detection	186:200	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	19	theme	early	186:190	arg1	monitoring					218:227	progression monitoring	206:227	progression monitoring	206:227	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	19	theme	early	186:190	arg1	issues					248:253	the most vital issues	233:253	the most vital issues in patients' treatment and mortality reduction	233:300	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	0	20	theme	oxide-chitosan-gold	101:119	arg1	composite					134:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	a reduced graphene oxide-chitosan-gold nanoparticle composite	82:142	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	4	21	theme	reduced	495:501	arg1	substrate					584:592	a biocompatible substrate	568:592	a biocompatible substrate for the receptor stabilization	568:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	4	21	theme	reduced	495:501	arg1	composite					546:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	3	22	theme	graphene-based	404:417	arg1	aptasensor					419:428	a graphene-based aptasensor	402:428	a graphene-based aptasensor	402:428	Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection.					
33845441	3	23	theme	sensitive	447:455	arg1	detection					482:490	sensitive human breast cancer cell detection	447:490	sensitive human breast cancer cell detection	447:490	Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection.					
33845441	0	24	theme	label-free	2:11	arg1	aptasensor					29:38	A label-free electrochemical aptasensor	0:38	A label-free electrochemical aptasensor for breast cancer cell detection	0:71	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	6	25	from	capture	1047:1053	arg1	aptasensor					1062:1071	the aptasensor	1058:1071	the aptasensor	1058:1071	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	9	26	theme	linear	1416:1421	arg1	relationship					1423:1434	a linear relationship	1414:1434	a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL	1414:1580	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	2	27	theme	great	325:329	arg1	demand					331:336	a great demand	323:336	a great demand for fast, inexpensive, and selective detection methods	323:391	Therefore, there is a great demand for fast, inexpensive, and selective detection methods.					
33845441	9	28	theme	low	1548:1550	arg1	limit					1562:1566	a low detection limit	1546:1566	a low detection limit of 4 cells/mL	1546:1580	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	5	29	theme	surface	845:851	arg1	area					853:856	effective surface area	835:856	effective surface area	835:856	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	8	30	theme	captured	1246:1253	arg1	cells					1255:1259	the higher captured cells	1235:1259	the higher captured cells	1235:1259	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	4	31	used	used	560:563	arg2	composite					546:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	4	31	used	used	560:563	arg2	substrate					584:592	a biocompatible substrate	568:592	a biocompatible substrate for the receptor stabilization	568:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	7	32	theme	electrochemical	1151:1165	arg1	spectroscopy					1177:1188	electrochemical impedance spectroscopy	1151:1188	electrochemical impedance spectroscopy	1151:1188	The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy.					
33845441	2	33	theme	detection	375:383	arg1	methods					385:391	fast, inexpensive, and selective detection methods	342:391	fast, inexpensive, and selective detection methods	342:391	Therefore, there is a great demand for fast, inexpensive, and selective detection methods.					
33845441	4	34	theme	biocompatible	570:582	arg1	substrate					584:592	a biocompatible substrate	568:592	a biocompatible substrate for the receptor stabilization	568:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	4	34	theme	biocompatible	570:582	arg1	composite					546:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	5	35	theme	components	725:734	arg1	effects					710:716	the synergistic effects	694:716	the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area	694:856	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	9	36	with	relationship	1423:1434	arg1	logarithm					1464:1472	the cell concentration logarithm	1441:1472	the cell concentration logarithm	1441:1472	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	36	with	relationship	1423:1434	arg1	range					1507:1511	a wide linear range	1493:1511	a wide linear range of 1 × 101-1 × 106 cells/mL	1493:1539	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	36	with	relationship	1423:1434	arg1	selectivity					1480:1490	high selectivity	1475:1490	high selectivity	1475:1490	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	36	with	relationship	1423:1434	arg1	limit					1562:1566	a low detection limit	1546:1566	a low detection limit of 4 cells/mL	1546:1580	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	3	37	theme	cancer	470:475	arg1	detection					482:490	sensitive human breast cancer cell detection	447:490	sensitive human breast cancer cell detection	447:490	Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection.					
33845441	0	38	theme	electrochemical	13:27	arg1	aptasensor					29:38	A label-free electrochemical aptasensor	0:38	A label-free electrochemical aptasensor for breast cancer cell detection	0:71	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	6	39	theme	aptamer	1016:1022	arg1	dimensional-structure					980:1000	the three dimensional-structure	970:1000	the three dimensional-structure of the AS1411 aptamer	970:1022	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	7	40	theme	cyclic	1128:1133	arg1	voltammetry					1135:1145	cyclic voltammetry	1128:1145	cyclic voltammetry	1128:1145	The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy.					
33845441	9	41	theme	linear	1500:1505	arg1	range					1507:1511	a wide linear range	1493:1511	a wide linear range of 1 × 101-1 × 106 cells/mL	1493:1539	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	5	42	from	function	642:649	arg1	composite					674:682	this composite	669:682	this composite	669:682	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	5	43	theme	effective	835:843	arg1	area					853:856	effective surface area	835:856	effective surface area	835:856	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	1	44	theme	cancer	159:164	arg1	consequences					172:183	the cancer fatal consequences	155:183	the cancer fatal consequences	155:183	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	0	45	theme	breast	44:49	arg1	detection					63:71	breast cancer cell detection	44:71	breast cancer cell detection	44:71	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	6	46	theme	AS1411	1009:1014	arg1	aptamer					1016:1022	the AS1411 aptamer	1005:1022	the AS1411 aptamer	1005:1022	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	9	47	theme	detection	1552:1560	arg1	limit					1562:1566	a low detection limit	1546:1566	a low detection limit of 4 cells/mL	1546:1580	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	1	48	theme	progression	206:216	arg1	issues					248:253	the most vital issues	233:253	the most vital issues in patients' treatment and mortality reduction	233:300	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	48	theme	progression	206:216	arg1	monitoring					218:227	progression monitoring	206:227	progression monitoring	206:227	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	48	theme	progression	206:216	arg1	detection					192:200	early detection	186:200	early detection	186:200	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	3	49	theme	human	457:461	arg1	detection					482:490	sensitive human breast cancer cell detection	447:490	sensitive human breast cancer cell detection	447:490	Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection.					
33845441	9	50	theme	cell	1445:1448	arg1	logarithm					1464:1472	the cell concentration logarithm	1441:1472	the cell concentration logarithm	1441:1472	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	6	51	theme	cell	1042:1045	arg1	capture					1047:1053	the cell capture	1038:1053	the cell capture on the aptasensor	1038:1071	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	6	52	theme	cancer	900:905	arg1	cells					907:911	MCF-7 cancer cells	894:911	MCF-7 cancer cells	894:911	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	6	53	from	incubation	880:889	arg1	cells					907:911	MCF-7 cancer cells	894:911	MCF-7 cancer cells	894:911	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	3	54	theme	breast	463:468	arg1	detection					482:490	sensitive human breast cancer cell detection	447:490	sensitive human breast cancer cell detection	447:490	Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection.					
33845441	0	55	theme	cell	58:61	arg1	detection					63:71	breast cancer cell detection	44:71	breast cancer cell detection	44:71	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	8	56	theme	higher	1239:1244	arg1	cells					1255:1259	the higher captured cells	1235:1259	the higher captured cells	1235:1259	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	5	57	theme	synergistic	698:708	arg1	effects					710:716	the synergistic effects	694:716	the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area	694:856	The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area.					
33845441	6	58	theme	MCF-7	894:898	arg1	cells					907:911	MCF-7 cancer cells	894:911	MCF-7 cancer cells	894:911	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	6	59	theme	cell	918:921	arg1	proteins					932:939	the cell membrane proteins	914:939	the cell membrane proteins	914:939	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	8	60	theme	transfer	1370:1377	arg1	resistances					1379:1389	the charge transfer resistances	1359:1389	the charge transfer resistances	1359:1389	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	0	61	theme	cancer	51:56	arg1	detection					63:71	breast cancer cell detection	44:71	breast cancer cell detection	44:71	A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.					
33845441	7	62	theme	impedance	1167:1175	arg1	spectroscopy					1177:1188	electrochemical impedance spectroscopy	1151:1188	electrochemical impedance spectroscopy	1151:1188	The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy.					
33845441	2	63	theme	fast	342:345	arg1	methods					385:391	fast, inexpensive, and selective detection methods	342:391	fast, inexpensive, and selective detection methods	342:391	Therefore, there is a great demand for fast, inexpensive, and selective detection methods.					
33845441	8	64	theme	higher	1195:1200	arg1	concentrations					1207:1220	The higher cell concentrations	1191:1220	The higher cell concentrations	1191:1220	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	9	65	theme	cells/mL	1573:1580	arg1	range					1507:1511	a wide linear range	1493:1511	a wide linear range of 1 × 101-1 × 106 cells/mL	1493:1539	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	65	theme	cells/mL	1573:1580	arg1	logarithm					1464:1472	the cell concentration logarithm	1441:1472	the cell concentration logarithm	1441:1472	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	65	theme	cells/mL	1573:1580	arg1	selectivity					1480:1490	high selectivity	1475:1490	high selectivity	1475:1490	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	65	theme	cells/mL	1573:1580	arg1	limit					1562:1566	a low detection limit	1546:1566	a low detection limit of 4 cells/mL	1546:1580	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	66	theme	high	1475:1478	arg1	selectivity					1480:1490	high selectivity	1475:1490	high selectivity	1475:1490	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	1	67	theme	vital	242:246	arg1	issues					248:253	the most vital issues	233:253	the most vital issues in patients' treatment and mortality reduction	233:300	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	67	theme	vital	242:246	arg1	monitoring					218:227	progression monitoring	206:227	progression monitoring	206:227	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	67	theme	vital	242:246	arg1	detection					192:200	early detection	186:200	early detection	186:200	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	68	from	issues	248:253	arg1	treatment					268:276	patients' treatment	258:276	patients' treatment	258:276	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	1	68	from	issues	248:253	arg1	reduction					292:300	mortality reduction	282:300	mortality reduction	282:300	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	6	69	theme	aptasensor	869:878	arg1	incubation					880:889	the aptasensor incubation	865:889	the aptasensor incubation in MCF-7 cancer cells	865:911	After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor.					
33845441	7	70	theme	fabrication	1089:1099	arg1	steps					1101:1105	The aptasensor fabrication steps	1074:1105	The aptasensor fabrication steps	1074:1105	The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy.					
33845441	2	71	theme	selective	365:373	arg1	methods					385:391	fast, inexpensive, and selective detection methods	342:391	fast, inexpensive, and selective detection methods	342:391	Therefore, there is a great demand for fast, inexpensive, and selective detection methods.					
33845441	9	72	theme	cells/mL	1532:1539	arg1	range					1507:1511	a wide linear range	1493:1511	a wide linear range of 1 × 101-1 × 106 cells/mL	1493:1539	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	72	theme	cells/mL	1532:1539	arg1	logarithm					1464:1472	the cell concentration logarithm	1441:1472	the cell concentration logarithm	1441:1472	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	72	theme	cells/mL	1532:1539	arg1	selectivity					1480:1490	high selectivity	1475:1490	high selectivity	1475:1490	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	9	72	theme	cells/mL	1532:1539	arg1	limit					1562:1566	a low detection limit	1546:1566	a low detection limit of 4 cells/mL	1546:1580	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
33845441	8	73	theme	cell	1202:1205	arg1	concentrations					1207:1220	The higher cell concentrations	1191:1220	The higher cell concentrations	1191:1220	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	4	74	theme	oxide-chitosan-gold	512:530	arg1	substrate					584:592	a biocompatible substrate	568:592	a biocompatible substrate for the receptor stabilization	568:623	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	4	74	theme	oxide-chitosan-gold	512:530	arg1	composite					546:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite	493:554	A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization.					
33845441	8	75	from	increases	1346:1354	arg1	resistances					1379:1389	the charge transfer resistances	1359:1389	the charge transfer resistances	1359:1389	The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances.					
33845441	1	76	theme	fatal	166:170	arg1	consequences					172:183	the cancer fatal consequences	155:183	the cancer fatal consequences	155:183	Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction.					
33845441	9	77	theme	concentration	1450:1462	arg1	logarithm					1464:1472	the cell concentration logarithm	1441:1472	the cell concentration logarithm	1441:1472	This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 101-1 × 106 cells/mL, and a low detection limit of 4 cells/mL.					
34678384	7	0	theme	guided	1234:1239	arg1	nanomachines					1241:1252	These magnetically guided nanomachines	1215:1252	These magnetically guided nanomachines	1215:1252	These magnetically guided nanomachines demonstrated a practical and good sensing ability by recognizing dsDNA between 0.01 mg/L and 10 mg/L.					
34678384	4	1	theme	electron	725:732	arg1	microscopy					734:743	scanning electron microscopy	716:743	scanning electron microscopy (SEM)	716:749	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	1	theme	electron	725:732	arg1	SEM					746:748	SEM	746:748	SEM	746:748	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	2	theme	structural	876:885	arg1	morphology					887:896	structural morphology	876:896	structural morphology	876:896	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	9	3	theme	voltammetry	1456:1466	arg1	experiments					1522:1532	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments	1449:1532	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments	1449:1532	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	1	4	theme	gold‑nickel	187:197	arg1	nanomotors					218:227	nanomotors	218:227	nanomotors	218:227	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	4	theme	gold‑nickel	187:197	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	4	theme	gold‑nickel	187:197	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	5	theme	double-stranded	262:276	arg1	dsDNA					301:305	dsDNA	301:305	dsDNA	301:305	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	5	theme	double-stranded	262:276	arg1	acid					295:298	double-stranded deoxyribonucleic acid	262:298	motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition	249:318	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	2	6	theme	direct	424:429	arg1	DC					440:441	DC	440:441	DC	440:441	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	2	6	theme	direct	424:429	arg1	current					431:437	direct current	424:437	direct current (DC) magnetron sputtering over electrochemically deposited Au wires	424:505	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	1	7	theme	wire	199:202	arg1	nanomotors					218:227	nanomotors	218:227	nanomotors	218:227	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	7	theme	wire	199:202	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	7	theme	wire	199:202	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	8	theme	deoxyribonucleic	278:293	arg1	dsDNA					301:305	dsDNA	301:305	dsDNA	301:305	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	8	theme	deoxyribonucleic	278:293	arg1	acid					295:298	double-stranded deoxyribonucleic acid	262:298	motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition	249:318	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	4	9	theme	mobility	941:948	arg1	elucidation					861:871	the elucidation	857:871	the elucidation of structural morphology, elemental composition and electrophoretic mobility	857:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	6	10	theme	magnetic	1199:1206	arg1	field					1208:1212	22 mT magnetic field	1193:1212	22 mT magnetic field	1193:1212	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	1	11	theme	acid	295:298	arg1	recognition					308:318	motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition	249:318	motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition	249:318	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	4	12	theme	morphology	887:896	arg1	elucidation					861:871	the elucidation	857:871	the elucidation of structural morphology, elemental composition and electrophoretic mobility	857:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	3	13	theme	drop	586:589	arg1	casting					591:597	drop casting	586:597	drop casting which could provide a novel and functional surface for leading bio-applications	586:677	Subsequently, biopolymer chitosan was dispersed onto this bimetallic layer by drop casting which could provide a novel and functional surface for leading bio-applications.					
34678384	6	14	theme	CS	1149:1150	arg1	19 μm/s					1179:1185	19 μm/s	1179:1185	19 μm/s	1179:1185	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	6	14	theme	CS	1149:1150	arg1	NMs					1158:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	3	15	theme	biopolymer	522:531	arg1	chitosan					533:540	biopolymer chitosan	522:540	biopolymer chitosan	522:540	Subsequently, biopolymer chitosan was dispersed onto this bimetallic layer by drop casting which could provide a novel and functional surface for leading bio-applications.					
34678384	3	16	theme	bimetallic	566:575	arg1	layer					577:581	this bimetallic layer	561:581	this bimetallic layer	561:581	Subsequently, biopolymer chitosan was dispersed onto this bimetallic layer by drop casting which could provide a novel and functional surface for leading bio-applications.					
34678384	1	17	theme	nanomachines	204:215	arg1	preparation					148:158	the preparation	144:158	the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection	144:332	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	9	18	theme	impedance	1493:1501	arg1	EIS					1517:1519	EIS	1517:1519	EIS	1517:1519	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	9	18	theme	impedance	1493:1501	arg1	spectroscopy					1503:1514	electrochemical impedance spectroscopy	1477:1514	electrochemical impedance spectroscopy (EIS)	1477:1520	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	8	19	theme	Electrochemical	1356:1370	arg1	characterization					1372:1387	Electrochemical characterization	1356:1387	Electrochemical characterization	1356:1387	Electrochemical characterization was also performed to identify the surface characteristics.					
34678384	4	20	theme	X-ray	792:796	arg1	XRD					811:813	XRD	811:813	XRD	811:813	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	20	theme	X-ray	792:796	arg1	diffraction					798:808	X-ray diffraction	792:808	X-ray diffraction (XRD)	792:814	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	21	theme	@	682:682	arg1	NMs					689:691	CS@Au-Ni NMs	680:691	CS@Au-Ni NMs	680:691	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	7	22	theme	good	1283:1286	arg1	ability					1296:1302	a practical and good sensing ability	1267:1302	a practical and good sensing ability	1267:1302	These magnetically guided nanomachines demonstrated a practical and good sensing ability by recognizing dsDNA between 0.01 mg/L and 10 mg/L.					
34678384	8	23	theme	surface	1424:1430	arg1	characteristics					1432:1446	the surface characteristics	1420:1446	the surface characteristics	1420:1446	Electrochemical characterization was also performed to identify the surface characteristics.					
34678384	9	24	theme	NMs	1567:1569	arg1	interaction					1548:1558	the interaction	1544:1558	the interaction of the NMs with dsDNA	1544:1580	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	9	25	theme	electrochemical	1477:1491	arg1	EIS					1517:1519	EIS	1517:1519	EIS	1517:1519	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	9	25	theme	electrochemical	1477:1491	arg1	spectroscopy					1503:1514	electrochemical impedance spectroscopy	1477:1514	electrochemical impedance spectroscopy (EIS)	1477:1520	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	4	26	theme	photoelectron	758:770	arg1	XPS					786:788	XPS	786:788	XPS	786:788	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	26	theme	photoelectron	758:770	arg1	spectroscopy					772:783	X-ray photoelectron spectroscopy	752:783	X-ray photoelectron spectroscopy (XPS)	752:789	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	2	27	theme	layer	402:406	arg1	formation					408:416	Ni layer formation	399:416	Ni layer formation	399:416	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	0	28	theme	bimetallic	36:45	arg1	nanomachines					56:67	gold-nickel bimetallic magnetic nanomachines	24:67	gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition	24:118	Chitosan functionalized gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition.					
34678384	4	29	theme	potential	826:834	arg1	methods					845:851	zeta potential analysis methods	821:851	zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility	821:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	1	30	theme	present	129:135	arg1	study					137:141	this present study	124:141	this present study	124:141	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	9	31	theme	spectroscopy	1503:1514	arg1	experiments					1522:1532	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments	1449:1532	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments	1449:1532	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	4	32	theme	CS	680:681	arg1	NMs					689:691	CS@Au-Ni NMs	680:691	CS@Au-Ni NMs	680:691	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	0	33	theme	gold-nickel	24:34	arg1	nanomachines					56:67	gold-nickel bimetallic magnetic nanomachines	24:67	gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition	24:118	Chitosan functionalized gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition.					
34678384	2	34	theme	Ni	399:400	arg1	formation					408:416	Ni layer formation	399:416	Ni layer formation	399:416	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	1	35	theme	CS	231:232	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	35	theme	CS	231:232	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	4	36	theme	Au-Ni	683:687	arg1	NMs					689:691	CS@Au-Ni NMs	680:691	CS@Au-Ni NMs	680:691	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	37	theme	zeta	821:824	arg1	methods					845:851	zeta potential analysis methods	821:851	zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility	821:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	1	38	theme	@	233:233	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	38	theme	@	233:233	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	0	39	theme	magnetic	47:54	arg1	nanomachines					56:67	gold-nickel bimetallic magnetic nanomachines	24:67	gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition	24:118	Chitosan functionalized gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition.					
34678384	3	40	theme	functional	631:640	arg1	surface					642:648	a novel and functional surface	619:648	a novel and functional surface for leading bio-applications	619:677	Subsequently, biopolymer chitosan was dispersed onto this bimetallic layer by drop casting which could provide a novel and functional surface for leading bio-applications.					
34678384	1	41	theme	Au-Ni	234:238	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	41	theme	Au-Ni	234:238	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	2	42	theme	Au	498:499	arg1	wires					501:505	electrochemically deposited Au wires	470:505	electrochemically deposited Au wires	470:505	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	5	43	from	changes	1092:1098	arg1	velocities					1109:1118	their velocities	1103:1118	their velocities	1103:1118	On account of presenting the application of these magnetic nanomachines, they were interacted with different concentrations of dsDNA and the changes in their velocities were investigated.					
34678384	4	44	theme	composition	909:919	arg1	elucidation					861:871	the elucidation	857:871	the elucidation of structural morphology, elemental composition and electrophoretic mobility	857:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	0	45	theme	deoxyribonucleic	86:101	arg1	recognition					108:118	motion-based deoxyribonucleic acid recognition	73:118	motion-based deoxyribonucleic acid recognition	73:118	Chitosan functionalized gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition.					
34678384	4	46	theme	analysis	836:843	arg1	methods					845:851	zeta potential analysis methods	821:851	zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility	821:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	6	47	theme	speed	1143:1147	arg1	19 μm/s					1179:1185	19 μm/s	1179:1185	19 μm/s	1179:1185	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	6	47	theme	speed	1143:1147	arg1	NMs					1158:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	2	48	theme	nanomachines	366:377	arg1	Synthesis					349:357	Synthesis	349:357	Synthesis of the nanomachines	349:377	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	2	49	theme	deposited	488:496	arg1	wires					501:505	electrochemically deposited Au wires	470:505	electrochemically deposited Au wires	470:505	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	6	50	theme	mT	1196:1197	arg1	field					1208:1212	22 mT magnetic field	1193:1212	22 mT magnetic field	1193:1212	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	4	51	theme	elemental	899:907	arg1	composition					909:919	elemental composition	899:919	elemental composition	899:919	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	5	52	theme	dsDNA	1078:1082	arg1	concentrations					1060:1073	different concentrations	1050:1073	different concentrations of dsDNA	1050:1082	On account of presenting the application of these magnetic nanomachines, they were interacted with different concentrations of dsDNA and the changes in their velocities were investigated.					
34678384	3	53	theme	novel	621:625	arg1	surface					642:648	a novel and functional surface	619:648	a novel and functional surface for leading bio-applications	619:677	Subsequently, biopolymer chitosan was dispersed onto this bimetallic layer by drop casting which could provide a novel and functional surface for leading bio-applications.					
34678384	6	54	theme	Au-Ni	1152:1156	arg1	19 μm/s					1179:1185	19 μm/s	1179:1185	19 μm/s	1179:1185	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	6	54	theme	Au-Ni	1152:1156	arg1	NMs					1158:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	7	55	theme	sensing	1288:1294	arg1	ability					1296:1302	a practical and good sensing ability	1267:1302	a practical and good sensing ability	1267:1302	These magnetically guided nanomachines demonstrated a practical and good sensing ability by recognizing dsDNA between 0.01 mg/L and 10 mg/L.					
34678384	4	56	theme	electrophoretic	925:939	arg1	mobility					941:948	electrophoretic mobility	925:948	electrophoretic mobility	925:948	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	3	57	theme	leading	654:660	arg1	bio-applications					662:677	leading bio-applications	654:677	leading bio-applications	654:677	Subsequently, biopolymer chitosan was dispersed onto this bimetallic layer by drop casting which could provide a novel and functional surface for leading bio-applications.					
34678384	5	58	theme	different	1050:1058	arg1	concentrations					1060:1073	different concentrations	1050:1073	different concentrations of dsDNA	1050:1082	On account of presenting the application of these magnetic nanomachines, they were interacted with different concentrations of dsDNA and the changes in their velocities were investigated.					
34678384	4	59	theme	X-ray	752:756	arg1	XPS					786:788	XPS	786:788	XPS	786:788	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	59	theme	X-ray	752:756	arg1	spectroscopy					772:783	X-ray photoelectron spectroscopy	752:783	X-ray photoelectron spectroscopy (XPS)	752:789	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	9	60	theme	Cyclic	1449:1454	arg1	CV					1469:1470	CV	1469:1470	CV	1469:1470	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	9	60	theme	Cyclic	1449:1454	arg1	voltammetry					1456:1466	Cyclic voltammetry	1449:1466	Cyclic voltammetry (CV)	1449:1471	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	5	61	theme	nanomachines	1010:1021	arg1	application					980:990	the application	976:990	the application of these magnetic nanomachines	976:1021	On account of presenting the application of these magnetic nanomachines, they were interacted with different concentrations of dsDNA and the changes in their velocities were investigated.					
34678384	9	62	theme	convenient	1600:1609	arg1	recognition					1611:1621	the convenient recognition	1596:1621	the convenient recognition	1596:1621	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	5	63	theme	magnetic	1001:1008	arg1	nanomachines					1010:1021	these magnetic nanomachines	995:1021	these magnetic nanomachines	995:1021	On account of presenting the application of these magnetic nanomachines, they were interacted with different concentrations of dsDNA and the changes in their velocities were investigated.					
34678384	4	64	theme	scanning	716:723	arg1	microscopy					734:743	scanning electron microscopy	716:743	scanning electron microscopy (SEM)	716:749	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	4	64	theme	scanning	716:723	arg1	SEM					746:748	SEM	746:748	SEM	746:748	CS@Au-Ni NMs were characterized via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and zeta potential analysis methods for the elucidation of structural morphology, elemental composition and electrophoretic mobility.					
34678384	6	65	theme	@	1151:1151	arg1	19 μm/s					1179:1185	19 μm/s	1179:1185	19 μm/s	1179:1185	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	6	65	theme	@	1151:1151	arg1	NMs					1158:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs	1139:1160	The speed CS@Au-Ni NMs were measured as 19 μm/s under 22 mT magnetic field.					
34678384	0	66	theme	acid	103:106	arg1	recognition					108:118	motion-based deoxyribonucleic acid recognition	73:118	motion-based deoxyribonucleic acid recognition	73:118	Chitosan functionalized gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition.					
34678384	2	67	theme	current	431:437	arg1	magnetron					444:452	direct current (DC) magnetron	424:452	direct current (DC) magnetron sputtering over electrochemically deposited Au wires	424:505	Synthesis of the nanomachines was accomplished by Ni layer formation using direct current (DC) magnetron sputtering over electrochemically deposited Au wires.					
34678384	9	68	with	interaction	1548:1558	arg1	dsDNA					1576:1580	dsDNA	1576:1580	dsDNA	1576:1580	Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments presented the interaction of the NMs with dsDNA by indicating the convenient recognition.					
34678384	1	69	theme	chitosan	163:170	arg1	nanomotors					218:227	nanomotors	218:227	nanomotors	218:227	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	69	theme	chitosan	163:170	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	69	theme	chitosan	163:170	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	0	70	theme	motion-based	73:84	arg1	recognition					108:118	motion-based deoxyribonucleic acid recognition	73:118	motion-based deoxyribonucleic acid recognition	73:118	Chitosan functionalized gold-nickel bimetallic magnetic nanomachines for motion-based deoxyribonucleic acid recognition.					
34678384	1	71	theme	functionalized	172:185	arg1	nanomotors					218:227	nanomotors	218:227	nanomotors	218:227	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	71	theme	functionalized	172:185	arg1	nanomachines					204:215	chitosan functionalized gold‑nickel wire nanomachines	163:215	chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs)	163:243	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	1	71	theme	functionalized	172:185	arg1	NMs					240:242	CS@Au-Ni NMs	231:242	CS@Au-Ni NMs	231:242	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
34678384	7	72	theme	practical	1269:1277	arg1	ability					1296:1302	a practical and good sensing ability	1267:1302	a practical and good sensing ability	1267:1302	These magnetically guided nanomachines demonstrated a practical and good sensing ability by recognizing dsDNA between 0.01 mg/L and 10 mg/L.					
34678384	1	73	theme	motion-based	249:260	arg1	recognition					308:318	motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition	249:318	motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition	249:318	In this present study, the preparation of chitosan functionalized gold‑nickel wire nanomachines (nanomotors) (CS@Au-Ni NMs) for motion-based double-stranded deoxyribonucleic acid (dsDNA) recognition and detection was described.					
32290315	8	0	theme	AuNP	1113:1116	arg1	2-Tyr					1133:1137	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr	1101:1137	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr	1101:1137	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	9	1	theme	improved	1560:1567	arg1	performances					1569:1580	improved performances	1560:1580	improved performances	1560:1580	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	6	2	theme	Electrochemical	725:739	arg1	responses					741:749	Electrochemical responses	725:749	Electrochemical responses towards catechol	725:766	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	3	3	theme	electrocatalytic	485:500	arg1	materials					502:510	anionic electrocatalytic materials	477:510	anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	477:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	3	theme	electrocatalytic	485:500	arg1	CuPcS					547:551	CuPcS	547:551	CuPcS	547:551	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	3	theme	electrocatalytic	485:500	arg1	nanoparticles					561:573	gold nanoparticles	556:573	gold nanoparticles	556:573	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	3	theme	electrocatalytic	485:500	arg1	phthalocyanine					531:544	sulfonated copper phthalocyanine	513:544	sulfonated copper phthalocyanine	513:544	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	9	4	theme	platform	1500:1507	arg1	structure					1483:1491	the structure	1479:1491	the structure of the platform	1479:1507	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	8	5	theme	The	1101:1103	arg1	2-Tyr					1133:1137	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr	1101:1137	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr	1101:1137	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	3	6	theme	materials	502:510	arg1	layers					467:472	layers	467:472	layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	467:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	8	7	theme	magnitude	1298:1306	arg1	orders					1288:1293	two orders	1284:1293	two orders of magnitude	1284:1306	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	5	8	theme	surface	667:673	arg1	roughness					675:683	surface roughness	667:683	surface roughness	667:683	Characterization of surface roughness and porosity was carried out using AFM.					
32290315	3	9	theme	gold	556:559	arg1	materials					502:510	anionic electrocatalytic materials	477:510	anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	477:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	9	theme	gold	556:559	arg1	nanoparticles					561:573	gold nanoparticles	556:573	gold nanoparticles	556:573	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	0	10	theme	Catechol	112:119	arg1	Detection					99:107	the Electrochemical Detection	79:107	the Electrochemical Detection of Catechol	79:119	Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol.					
32290315	5	11	theme	roughness	675:683	arg1	Characterization					647:662	Characterization	647:662	Characterization of surface roughness and porosity	647:696	Characterization of surface roughness and porosity was carried out using AFM.					
32290315	6	12	theme	transfer	833:840	arg1	path					842:845	the electron transfer path	820:845	the electron transfer path between Tyr or Lac and the electrode surface	820:890	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	2	13	theme	catechol	311:318	arg1	biosensors					320:329	improved catechol biosensors	302:329	improved catechol biosensors	302:329	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	9	14	with	biosensors	1544:1553	arg1	performances					1569:1580	improved performances	1560:1580	improved performances	1560:1580	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	6	15	theme	electron	824:831	arg1	path					842:845	the electron transfer path	820:845	the electron transfer path between Tyr or Lac and the electrode surface	820:890	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	2	16	theme	improved	302:309	arg1	biosensors					320:329	improved catechol biosensors	302:329	improved catechol biosensors	302:329	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	8	17	theme	8.55∙10-4	1156:1164	arg1	μM					1166:1167	8.55∙10-4 μM	1156:1167	8.55∙10-4 μM	1156:1167	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	6	18	from	increase	906:913	arg1	intensity					922:930	the intensity	918:930	the intensity over the response in the absence of the LbL platform	918:983	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	2	19	theme	Biosensor	157:165	arg1	platforms					167:175	Biosensor platforms	157:175	Biosensor platforms consisting of layer by layer films combining materials with different functionalities	157:261	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	2	20	used	used	287:290	arg2	platforms					167:175	Biosensor platforms	157:175	Biosensor platforms consisting of layer by layer films combining materials with different functionalities	157:261	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	8	21	dep	2-Tyr	1273:1277	arg1	CHI					1261:1263	(CHI)-	1260:1265	(CHI)-(AuNP)	1260:1271	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	8	21	dep	2-Tyr	1273:1277	arg1	CuPcS					1253:1257	CuPcS	1253:1257	CuPcS	1253:1257	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	8	21	dep	2-Tyr	1273:1277	arg1	AuNP					1267:1270	AuNP	1267:1270	AuNP	1267:1270	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	8	21	dep	2-Tyr	1273:1277	arg1	CHI					1247:1249	CHI)-	1247:1251	CHI)-(CuPcS)	1247:1258	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	9	22	theme	structure	1483:1491	arg1	combination					1398:1408	the combination	1394:1408	the combination of adequate materials with complementary activity	1394:1458	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	9	22	theme	structure	1483:1491	arg1	strategy					1525:1532	an excellent strategy	1512:1532	an excellent strategy to obtain biosensors with improved performances	1512:1580	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	9	22	theme	structure	1483:1491	arg1	control					1468:1474	the control	1464:1474	the control of the structure of the platform	1464:1507	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	8	23	theme	nanostructured	1343:1356	arg1	platforms					1358:1366	other nanostructured platforms	1337:1366	other nanostructured platforms	1337:1366	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	6	24	from	response	941:948	arg1	absence					957:963	the absence	953:963	the absence of the LbL platform	953:983	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	3	25	theme	cationic	418:425	arg1	chitosan					435:442	chitosan	435:442	chitosan	435:442	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	25	theme	cationic	418:425	arg1	linker					427:432	a cationic linker	416:432	a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	416:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	6	26	theme	platform	976:983	arg1	absence					957:963	the absence	953:963	the absence of the LbL platform	953:983	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	7	27	theme	lower	1089:1093	arg1	LODs					1095:1098	lower LODs	1089:1098	lower LODs	1089:1098	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	3	28	theme	linker	427:432	arg1	layers					406:411	layers	406:411	layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	406:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	9	29	with	materials	1422:1430	arg1	activity					1451:1458	complementary activity	1437:1458	complementary activity	1437:1458	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	6	30	theme	LbL	972:974	arg1	platform					976:983	the LbL platform	968:983	the LbL platform	968:983	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	3	31	dep	chitosan	435:442	arg1	CHI					445:447	CHI	445:447	CHI	445:447	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	32	theme	LbL	386:388	arg1	films					390:394	LbL films	386:394	LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	386:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	33	theme	copper	524:529	arg1	materials					502:510	anionic electrocatalytic materials	477:510	anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	477:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	33	theme	copper	524:529	arg1	phthalocyanine					531:544	sulfonated copper phthalocyanine	513:544	sulfonated copper phthalocyanine	513:544	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	2	34	theme	layer	200:204	arg1	films					206:210	layer films	200:210	layer films combining materials with different functionalities	200:261	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	2	35	with	materials	222:230	arg1	functionalities					247:261	different functionalities	237:261	different functionalities	237:261	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	8	36	theme	μM	1166:1167	arg1	LOD					1149:1151	an LOD	1146:1151	an LOD	1146:1151	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	8	36	theme	μM	1166:1167	arg1	lower					1203:1207	lower	1203:1207	lower	1203:1207	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	0	37	theme	Composite	59:67	arg1	Films					69:73	Chitosan/AuNPs/Phthalocyanine Composite Films	29:73	Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol	29:119	Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol.					
32290315	3	38	dep	phthalocyanine	531:544	arg1	AuNP					576:579	AuNP	576:579	AuNP	576:579	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	0	39	theme	Chitosan/AuNPs/Phthalocyanine	29:57	arg1	Films					69:73	Chitosan/AuNPs/Phthalocyanine Composite Films	29:73	Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol	29:119	Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol.					
32290315	7	40	theme	catechol	1066:1073	arg1	diffusion					1053:1061	the diffusion	1049:1061	the diffusion of catechol	1049:1073	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	6	41	theme	electrode	874:882	arg1	surface					884:890	the electrode surface	870:890	the electrode surface	870:890	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	6	42	theme	LbL	784:786	arg1	composites					788:797	the LbL composites	780:797	the LbL composites	780:797	Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform.					
32290315	9	43	theme	excellent	1515:1523	arg1	combination					1398:1408	the combination	1394:1408	the combination of adequate materials with complementary activity	1394:1458	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	9	43	theme	excellent	1515:1523	arg1	strategy					1525:1532	an excellent strategy	1512:1532	an excellent strategy to obtain biosensors with improved performances	1512:1580	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	9	43	theme	excellent	1515:1523	arg1	control					1468:1474	the control	1464:1474	the control of the structure of the platform	1464:1507	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	8	44	theme	other	1337:1341	arg1	platforms					1358:1366	other nanostructured platforms	1337:1366	other nanostructured platforms	1337:1366	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	7	45	theme	pore	1027:1030	arg1	size					1032:1035	pore size	1027:1035	pore size	1027:1035	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	7	46	theme	LbL	986:988	arg1	structures					990:999	LbL structures	986:999	LbL structures with higher roughness and pore size	986:1035	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	5	47	theme	porosity	689:696	arg1	Characterization					647:662	Characterization	647:662	Characterization of surface roughness and porosity	647:696	Characterization of surface roughness and porosity was carried out using AFM.					
32290315	2	48	theme	different	237:245	arg1	functionalities					247:261	different functionalities	237:261	different functionalities	237:261	Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors.					
32290315	7	49	theme	higher	1006:1011	arg1	roughness					1013:1021	higher roughness	1006:1021	higher roughness	1006:1021	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	3	50	theme	anionic	477:483	arg1	materials					502:510	anionic electrocatalytic materials	477:510	anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	477:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	50	theme	anionic	477:483	arg1	CuPcS					547:551	CuPcS	547:551	CuPcS	547:551	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	50	theme	anionic	477:483	arg1	nanoparticles					561:573	gold nanoparticles	556:573	gold nanoparticles	556:573	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	50	theme	anionic	477:483	arg1	phthalocyanine					531:544	sulfonated copper phthalocyanine	513:544	sulfonated copper phthalocyanine	513:544	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	9	51	theme	adequate	1413:1420	arg1	materials					1422:1430	adequate materials	1413:1430	adequate materials with complementary activity	1413:1458	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	4	52	with	Films	583:587	arg1	structures					610:619	different layer structures	594:619	different layer structures	594:619	Films with different layer structures were successfully formed.					
32290315	1	53	theme	Surface	138:144	arg1	Structure					146:154	the Surface Structure	134:154	the Surface Structure	134:154	The Role of the Surface Structure.					
32290315	4	54	theme	different	594:602	arg1	structures					610:619	different layer structures	594:619	different layer structures	594:619	Films with different layer structures were successfully formed.					
32290315	9	55	theme	materials	1422:1430	arg1	combination					1398:1408	the combination	1394:1408	the combination of adequate materials with complementary activity	1394:1458	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	9	55	theme	materials	1422:1430	arg1	strategy					1525:1532	an excellent strategy	1512:1532	an excellent strategy to obtain biosensors with improved performances	1512:1580	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	9	55	theme	materials	1422:1430	arg1	control					1468:1474	the control	1464:1474	the control of the structure of the platform	1464:1507	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	1	56	theme	Structure	146:154	arg1	Role					126:129	The Role	122:129	The Role of the Surface Structure	122:154	The Role of the Surface Structure.					
32290315	8	57	theme	magnitude	1193:1201	arg1	order					1184:1188	one order	1180:1188	one order of magnitude	1180:1201	The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10-4 μM, which was one order of magnitude lower than the 9.55·10-3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms.					
32290315	0	58	theme	Electrochemical	83:97	arg1	Detection					99:107	the Electrochemical Detection	79:107	the Electrochemical Detection of Catechol	79:119	Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol.					
32290315	0	59	dep	Platform	11:18	arg1	Based					20:24	Based	20:24	Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol	11:119	Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol.					
32290315	7	60	with	structures	990:999	arg1	roughness					1013:1021	higher roughness	1006:1021	higher roughness	1006:1021	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	7	60	with	structures	990:999	arg1	size					1032:1035	pore size	1027:1035	pore size	1027:1035	LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs.					
32290315	9	61	theme	complementary	1437:1449	arg1	activity					1451:1458	complementary activity	1437:1458	complementary activity	1437:1458	It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.					
32290315	3	62	theme	sulfonated	513:522	arg1	materials					502:510	anionic electrocatalytic materials	477:510	anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP)	477:580	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	3	62	theme	sulfonated	513:522	arg1	phthalocyanine					531:544	sulfonated copper phthalocyanine	513:544	sulfonated copper phthalocyanine	513:544	Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP).					
32290315	4	63	theme	layer	604:608	arg1	structures					610:619	different layer structures	594:619	different layer structures	594:619	Films with different layer structures were successfully formed.					
32683466	4	0	theme	intestinal	714:723	arg1	diversity					735:743	intestinal microbial diversity	714:743	intestinal microbial diversity	714:743	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	8	1	theme	prebiotic	1507:1515	arg1	treatment					1517:1525	prebiotic treatment	1507:1525	prebiotic treatment	1507:1525	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	1	2	theme	Pacific	257:263	arg1	shrimp					271:276	Pacific white shrimp	257:276	Pacific white shrimp	257:276	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	7	3	theme	prebiotic	1332:1340	arg1	administration					1342:1355	prebiotic administration	1332:1355	prebiotic administration	1332:1355	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	3	4	theme	probiotic	578:586	arg1	addition					566:573	addition	566:573	addition of probiotic and prebiotic	566:600	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	6	5	theme	conversion	1076:1085	arg1	ratio					1087:1091	feed conversion ratio	1071:1091	feed conversion ratio of 1.45 ± 0.00	1071:1106	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	3	6	theme	prebiotic	474:482	arg1	%					457:457	0.5%	454:457	0.5% (v/w) of honey prebiotic	454:482	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	6	theme	prebiotic	474:482	arg1	prebiotic					474:482	honey prebiotic	468:482	honey prebiotic	468:482	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	6	theme	prebiotic	474:482	arg1	v/w					460:462	v/w	460:462	v/w	460:462	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	8	7	theme	Neptunomonas	1600:1611	arg1	diversity					1552:1560	the diversity	1548:1560	the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture	1548:1670	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	7	8	theme	microbiota	1297:1306	arg1	diversity					1308:1316	the intestinal microbiota diversity	1282:1316	the intestinal microbiota diversity	1282:1316	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	1	9	from	composition	242:252	arg1	shrimp					271:276	Pacific white shrimp	257:276	Pacific white shrimp	257:276	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	8	10	from	candidates	1646:1655	arg1	aquaculture					1660:1670	aquaculture	1660:1670	aquaculture	1660:1670	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	3	11	theme	prebiotic	592:600	arg1	addition					566:573	addition	566:573	addition of probiotic and prebiotic	566:600	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	6	12	theme	protein	1157:1163	arg1	3.09 ± 0.02					1048:1058	3.09 ± 0.02	1048:1058	3.09 ± 0.02 (% day-1)	1048:1068	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	12	theme	protein	1157:1163	arg1	day-1					1063:1067	% day-1	1061:1067	% day-1	1061:1067	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	12	theme	protein	1157:1163	arg1	activities					1120:1129	enzyme activities	1113:1129	enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1113:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	12	theme	protein	1157:1163	arg1	ratio					1087:1091	feed conversion ratio	1071:1091	feed conversion ratio of 1.45 ± 0.00	1071:1106	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	5	13	theme	amylase	905:911	arg1	activities					891:900	digestive enzyme activities	874:900	digestive enzyme activities of amylase, protease, and lipase	874:933	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	13	theme	amylase	905:911	arg1	ratio					863:867	feed conversion ratio	847:867	feed conversion ratio	847:867	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	13	theme	amylase	905:911	arg1	rate					841:844	specific growth rate	825:844	specific growth rate	825:844	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	8	14	theme	established	1624:1634	arg1	Lactobacillus					1581:1593	Lactobacillus	1581:1593	Lactobacillus	1581:1593	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	8	14	theme	established	1624:1634	arg1	Neptunomonas					1600:1611	Neptunomonas	1600:1611	Neptunomonas	1600:1611	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	8	14	theme	established	1624:1634	arg1	Microbacterium					1565:1578	Microbacterium	1565:1578	Microbacterium	1565:1578	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	8	14	theme	established	1624:1634	arg1	candidates					1646:1655	established probiotic candidates	1624:1655	established probiotic candidates in aquaculture	1624:1670	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	6	15	theme	%	1061:1061	arg1	3.09 ± 0.02					1048:1058	3.09 ± 0.02	1048:1058	3.09 ± 0.02 (% day-1)	1048:1068	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	15	theme	%	1061:1061	arg1	day-1					1063:1067	% day-1	1061:1067	% day-1	1061:1067	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	0	16	theme	Honey	132:136	arg1	Prebiotic					138:146	Honey Prebiotic	132:146	Honey Prebiotic	132:146	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	5	17	theme	protease	914:921	arg1	activities					891:900	digestive enzyme activities	874:900	digestive enzyme activities of amylase, protease, and lipase	874:933	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	17	theme	protease	914:921	arg1	ratio					863:867	feed conversion ratio	847:867	feed conversion ratio	847:867	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	17	theme	protease	914:921	arg1	rate					841:844	specific growth rate	825:844	specific growth rate	825:844	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	6	18	theme	3.09 ± 0.02	1048:1058	arg1	rate					1040:1043	growth rate	1033:1043	growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1033:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	19	theme	enzyme	1113:1118	arg1	activities					1120:1129	enzyme activities	1113:1129	enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1113:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	5	20	theme	digestive	874:882	arg1	activities					891:900	digestive enzyme activities	874:900	digestive enzyme activities of amylase, protease, and lipase	874:933	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	4	21	from	effects	660:666	arg1	performance					698:708	growth performance	691:708	growth performance	691:708	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	4	21	from	effects	660:666	arg1	diversity					735:743	intestinal microbial diversity	714:743	intestinal microbial diversity	714:743	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	5	22	theme	probiotic	770:778	arg1	administration					750:763	The administration	746:763	The administration of a probiotic, prebiotic, or synbiotic	746:803	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	7	23	theme	Proteobacteria	1481:1494	arg1	dominance					1457:1465	dominance	1457:1465	dominance of the phylum Proteobacteria	1457:1494	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	6	24	theme	rate	1040:1043	arg1	values					1023:1028	values	1023:1028	values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase	1023:1267	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	5	25	theme	prebiotic	781:789	arg1	administration					750:763	The administration	746:763	The administration of a probiotic, prebiotic, or synbiotic	746:803	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	8	26	theme	Microbacterium	1565:1578	arg1	diversity					1552:1560	the diversity	1548:1560	the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture	1548:1670	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	0	27	theme	White	66:70	arg1	Shrimp					72:77	Pacific White Shrimp	58:77	Pacific White Shrimp	58:77	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	5	28	theme	white	946:950	arg1	shrimp					952:957	Pacific white shrimp	938:957	Pacific white shrimp	938:957	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	8	29	theme	probiotic	1636:1644	arg1	Lactobacillus					1581:1593	Lactobacillus	1581:1593	Lactobacillus	1581:1593	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	8	29	theme	probiotic	1636:1644	arg1	Neptunomonas					1600:1611	Neptunomonas	1600:1611	Neptunomonas	1600:1611	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	8	29	theme	probiotic	1636:1644	arg1	Microbacterium					1565:1578	Microbacterium	1565:1578	Microbacterium	1565:1578	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	8	29	theme	probiotic	1636:1644	arg1	candidates					1646:1655	established probiotic candidates	1624:1655	established probiotic candidates in aquaculture	1624:1670	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	6	30	theme	0.055 ± 0.0004 IU mg-1	1178:1199	arg1	protein					1201:1207	0.055 ± 0.0004 IU mg-1 protein	1178:1207	0.055 ± 0.0004 IU mg-1 protein for protease	1178:1220	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	9	31	theme	probiotic	1677:1685	arg1	treatments					1713:1722	The probiotic, prebiotic, and synbiotic treatments	1673:1722	The probiotic, prebiotic, and synbiotic treatments	1673:1722	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	5	32	theme	specific	825:832	arg1	rate					841:844	specific growth rate	825:844	specific growth rate	825:844	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	2	33	theme	sp	419:420	arg1	%					399:399	probiotic (1%	387:399	probiotic (1% (v/w) of Bacillus sp	387:420	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	2	33	theme	sp	419:420	arg1	sp					419:420	Bacillus sp	410:420	Bacillus sp	410:420	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	2	33	theme	sp	419:420	arg1	v/w					402:404	v/w	402:404	v/w	402:404	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	0	34	theme	Growth	0:5	arg1	Performance					7:17	Growth Performance	0:17	Growth Performance	0:17	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	4	35	theme	Next-generation	604:618	arg1	NGS					632:634	NGS	632:634	NGS	632:634	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	4	35	theme	Next-generation	604:618	arg1	sequencing					620:629	Next-generation sequencing	604:629	Next-generation sequencing (NGS)	604:635	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	0	36	theme	Microbiota	34:43	arg1	Diversity					45:53	Intestinal Microbiota Diversity	23:53	Intestinal Microbiota Diversity	23:53	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	1	37	theme	prebiotic	301:309	arg1	treatment					325:333	probiotic, honey prebiotic, or synbiotic treatment	284:333	probiotic, honey prebiotic, or synbiotic treatment	284:333	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	7	38	theme	Bacteroidetes	1388:1400	arg1	dominance					1364:1372	dominance	1364:1372	dominance of the phylum Bacteroidetes	1364:1400	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	3	39	theme	prebiotic	443:451	arg1	RfR					427:429	NP5 RfR	423:429	NP5 RfR probiotic)	423:440	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	6	40	theme	0.152 ± 0.0025 IU mg-1	1227:1248	arg1	protein					1250:1256	0.152 ± 0.0025 IU mg-1 protein	1227:1256	0.152 ± 0.0025 IU mg-1 protein for lipase	1227:1267	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	9	41	theme	taxonomic	1756:1764	arg1	OTUs					1773:1776	OTUs	1773:1776	OTUs	1773:1776	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	9	41	theme	taxonomic	1756:1764	arg1	units					1766:1770	operational taxonomic units	1744:1770	operational taxonomic units (OTUs) significantly higher than control treatment	1744:1821	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	3	42	theme	prebiotic	534:542	arg1	prebiotic					534:542	prebiotic	534:542	prebiotic	534:542	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	42	theme	prebiotic	534:542	arg1	v/w					501:503	v/w	501:503	v/w	501:503	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	42	theme	prebiotic	534:542	arg1	probiotic					509:517	probiotic	509:517	probiotic	509:517	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	42	theme	prebiotic	534:542	arg1	%					498:498	1%	497:498	1% (v/w) of probiotic and 0.5% (v/w) prebiotic	497:542	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	1	43	theme	microbiota	231:240	arg1	composition					242:252	intestinal microbiota composition	220:252	intestinal microbiota composition	220:252	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	5	44	theme	feed	847:850	arg1	ratio					863:867	feed conversion ratio	847:867	feed conversion ratio	847:867	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	6	45	theme	1.45 ± 0.00	1096:1106	arg1	3.09 ± 0.02					1048:1058	3.09 ± 0.02	1048:1058	3.09 ± 0.02 (% day-1)	1048:1068	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	45	theme	1.45 ± 0.00	1096:1106	arg1	day-1					1063:1067	% day-1	1061:1067	% day-1	1061:1067	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	45	theme	1.45 ± 0.00	1096:1106	arg1	activities					1120:1129	enzyme activities	1113:1129	enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1113:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	45	theme	1.45 ± 0.00	1096:1106	arg1	ratio					1087:1091	feed conversion ratio	1071:1091	feed conversion ratio of 1.45 ± 0.00	1071:1106	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	46	theme	ratio	1087:1091	arg1	rate					1040:1043	growth rate	1033:1043	growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1033:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	3	47	theme	honey	468:472	arg1	prebiotic					474:482	honey prebiotic	468:482	honey prebiotic	468:482	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	5	48	from	increases	812:820	arg1	activities					891:900	digestive enzyme activities	874:900	digestive enzyme activities of amylase, protease, and lipase	874:933	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	48	from	increases	812:820	arg1	ratio					863:867	feed conversion ratio	847:867	feed conversion ratio	847:867	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	48	from	increases	812:820	arg1	rate					841:844	specific growth rate	825:844	specific growth rate	825:844	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	48	from	increases	812:820	arg1	shrimp					952:957	Pacific white shrimp	938:957	Pacific white shrimp	938:957	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	1	49	theme	white	265:269	arg1	shrimp					271:276	Pacific white shrimp	257:276	Pacific white shrimp	257:276	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	7	50	theme	diversity	1308:1316	arg1	Analysis					1270:1277	Analysis	1270:1277	Analysis of the intestinal microbiota diversity	1270:1316	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	4	51	theme	microbial	725:733	arg1	diversity					735:743	intestinal microbial diversity	714:743	intestinal microbial diversity	714:743	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	9	52	theme	higher	1793:1798	arg1	OTUs					1773:1776	OTUs	1773:1776	OTUs	1773:1776	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	9	52	theme	higher	1793:1798	arg1	units					1766:1770	operational taxonomic units	1744:1770	operational taxonomic units (OTUs) significantly higher than control treatment	1744:1821	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	2	53	theme	white	344:348	arg1	shrimp					350:355	Pacific white shrimp	336:355	Pacific white shrimp	336:355	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	6	54	theme	feed	1071:1074	arg1	ratio					1087:1091	feed conversion ratio	1071:1091	feed conversion ratio of 1.45 ± 0.00	1071:1106	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	8	55	theme	Lactobacillus	1581:1593	arg1	diversity					1552:1560	the diversity	1548:1560	the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture	1548:1670	Moreover, prebiotic treatment was able to increase the diversity of Microbacterium, Lactobacillus, and Neptunomonas, which are established probiotic candidates in aquaculture.					
32683466	7	56	theme	intestinal	1286:1295	arg1	diversity					1308:1316	the intestinal microbiota diversity	1282:1316	the intestinal microbiota diversity	1282:1316	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	4	57	theme	treatments	677:686	arg1	effects					660:666	the effects	656:666	the effects of these treatments on growth performance and intestinal microbial diversity	656:743	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	6	58	theme	greatest	1001:1008	arg1	effect					1010:1015	the greatest effect	997:1015	the greatest effect	997:1015	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	9	59	theme	control	1805:1811	arg1	treatment					1813:1821	control treatment	1805:1821	control treatment	1805:1821	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	3	60	theme	synbiotic	486:494	arg1	RfR					427:429	NP5 RfR	423:429	NP5 RfR probiotic)	423:440	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	4	61	theme	growth	691:696	arg1	performance					698:708	growth performance	691:708	growth performance	691:708	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	6	62	theme	prebiotic	964:972	arg1	treatment					974:982	The prebiotic treatment	960:982	The prebiotic treatment	960:982	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	6	63	theme	1.388 ± 0.0211 IU mg-1	1134:1155	arg1	protein					1157:1163	1.388 ± 0.0211 IU mg-1 protein	1134:1163	1.388 ± 0.0211 IU mg-1 protein	1134:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	4	64	used	used	641:644	arg2	NGS					632:634	NGS	632:634	NGS	632:634	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	4	64	used	used	641:644	arg2	sequencing					620:629	Next-generation sequencing	604:629	Next-generation sequencing (NGS)	604:635	Next-generation sequencing (NGS) was used to assess the effects of these treatments on growth performance and intestinal microbial diversity.					
32683466	1	65	theme	growth	197:202	arg1	performance					204:214	growth performance	197:214	growth performance	197:214	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	6	66	theme	activities	1120:1129	arg1	rate					1040:1043	growth rate	1033:1043	growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1033:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	1	67	from	performance	204:214	arg1	shrimp					271:276	Pacific white shrimp	257:276	Pacific white shrimp	257:276	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	7	68	theme	phylum	1474:1479	arg1	Proteobacteria					1481:1494	the phylum Proteobacteria	1470:1494	the phylum Proteobacteria	1470:1494	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	5	69	theme	enzyme	884:889	arg1	activities					891:900	digestive enzyme activities	874:900	digestive enzyme activities of amylase, protease, and lipase	874:933	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	3	70	theme	probiotic	509:517	arg1	prebiotic					534:542	prebiotic	534:542	prebiotic	534:542	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	70	theme	probiotic	509:517	arg1	v/w					501:503	v/w	501:503	v/w	501:503	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	70	theme	probiotic	509:517	arg1	probiotic					509:517	probiotic	509:517	probiotic	509:517	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	70	theme	probiotic	509:517	arg1	%					498:498	1%	497:498	1% (v/w) of probiotic and 0.5% (v/w) prebiotic	497:542	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	6	71	theme	growth	1033:1038	arg1	rate					1040:1043	growth rate	1033:1043	growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein	1033:1163	The prebiotic treatment demonstrated the greatest effect, with values of growth rate of 3.09 ± 0.02 (% day-1), feed conversion ratio of 1.45 ± 0.00, and enzyme activities of 1.388 ± 0.0211 IU mg-1 protein for amylase, 0.055 ± 0.0004 IU mg-1 protein for protease, and 0.152 ± 0.0025 IU mg-1 protein for lipase.					
32683466	3	72	dep	synbiotic	486:494	arg1	prebiotic					534:542	prebiotic	534:542	prebiotic	534:542	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	72	dep	synbiotic	486:494	arg1	v/w					501:503	v/w	501:503	v/w	501:503	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	72	dep	synbiotic	486:494	arg1	probiotic					509:517	probiotic	509:517	probiotic	509:517	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	72	dep	synbiotic	486:494	arg1	%					498:498	1%	497:498	1% (v/w) of probiotic and 0.5% (v/w) prebiotic	497:542	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	7	73	theme	synbiotic	1429:1437	arg1	treatments					1439:1448	the probiotic and synbiotic treatments	1411:1448	the probiotic and synbiotic treatments	1411:1448	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	0	74	from	Performance	7:17	arg1	Shrimp					72:77	Pacific White Shrimp	58:77	Pacific White Shrimp	58:77	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	7	75	theme	probiotic	1415:1423	arg1	treatments					1439:1448	the probiotic and synbiotic treatments	1411:1448	the probiotic and synbiotic treatments	1411:1448	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	0	76	theme	Probiotic	111:119	arg1	Bacterium					121:129	a Probiotic Bacterium	109:129	a Probiotic Bacterium	109:129	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	5	77	theme	lipase	928:933	arg1	activities					891:900	digestive enzyme activities	874:900	digestive enzyme activities of amylase, protease, and lipase	874:933	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	77	theme	lipase	928:933	arg1	ratio					863:867	feed conversion ratio	847:867	feed conversion ratio	847:867	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	5	77	theme	lipase	928:933	arg1	rate					841:844	specific growth rate	825:844	specific growth rate	825:844	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	3	78	dep	prebiotic	443:451	arg1	%					457:457	0.5%	454:457	0.5% (v/w) of honey prebiotic	454:482	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	78	dep	prebiotic	443:451	arg1	prebiotic					474:482	honey prebiotic	468:482	honey prebiotic	468:482	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	3	78	dep	prebiotic	443:451	arg1	v/w					460:462	v/w	460:462	v/w	460:462	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	0	79	from	Diversity	45:53	arg1	Shrimp					72:77	Pacific White Shrimp	58:77	Pacific White Shrimp	58:77	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	2	80	theme	probiotic	387:395	arg1	%					399:399	probiotic (1%	387:399	probiotic (1% (v/w) of Bacillus sp	387:420	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	2	80	theme	probiotic	387:395	arg1	sp					419:420	Bacillus sp	410:420	Bacillus sp	410:420	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	2	80	theme	probiotic	387:395	arg1	v/w					402:404	v/w	402:404	v/w	402:404	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	5	81	theme	Pacific	938:944	arg1	shrimp					952:957	Pacific white shrimp	938:957	Pacific white shrimp	938:957	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	2	82	theme	Bacillus	410:417	arg1	sp					419:420	Bacillus sp	410:420	Bacillus sp	410:420	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	0	83	theme	Intestinal	23:32	arg1	Diversity					45:53	Intestinal Microbiota Diversity	23:53	Intestinal Microbiota Diversity	23:53	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	1	84	theme	probiotic	284:292	arg1	treatment					325:333	probiotic, honey prebiotic, or synbiotic treatment	284:333	probiotic, honey prebiotic, or synbiotic treatment	284:333	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	5	85	theme	synbiotic	795:803	arg1	administration					750:763	The administration	746:763	The administration of a probiotic, prebiotic, or synbiotic	746:803	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	9	86	theme	synbiotic	1703:1711	arg1	treatments					1713:1722	The probiotic, prebiotic, and synbiotic treatments	1673:1722	The probiotic, prebiotic, and synbiotic treatments	1673:1722	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	5	87	theme	conversion	852:861	arg1	ratio					863:867	feed conversion ratio	847:867	feed conversion ratio	847:867	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	3	88	theme	NP5	423:425	arg1	RfR					427:429	NP5 RfR	423:429	NP5 RfR probiotic)	423:440	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	0	89	theme	Pacific	58:64	arg1	Shrimp					72:77	Pacific White Shrimp	58:77	Pacific White Shrimp	58:77	Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic.					
32683466	2	90	theme	Pacific	336:342	arg1	shrimp					350:355	Pacific white shrimp	336:355	Pacific white shrimp	336:355	Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp.					
32683466	1	91	theme	synbiotic	315:323	arg1	treatment					325:333	probiotic, honey prebiotic, or synbiotic treatment	284:333	probiotic, honey prebiotic, or synbiotic treatment	284:333	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	7	92	theme	phylum	1381:1386	arg1	Bacteroidetes					1388:1400	the phylum Bacteroidetes	1377:1400	the phylum Bacteroidetes	1377:1400	Analysis of the intestinal microbiota diversity revealed that prebiotic administration caused dominance of the phylum Bacteroidetes, whereas the probiotic and synbiotic treatments caused dominance of the phylum Proteobacteria.					
32683466	5	93	theme	growth	834:839	arg1	rate					841:844	specific growth rate	825:844	specific growth rate	825:844	The administration of a probiotic, prebiotic, or synbiotic led to increases in specific growth rate, feed conversion ratio, and digestive enzyme activities of amylase, protease, and lipase in Pacific white shrimp.					
32683466	9	94	theme	operational	1744:1754	arg1	OTUs					1773:1776	OTUs	1773:1776	OTUs	1773:1776	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	9	94	theme	operational	1744:1754	arg1	units					1766:1770	operational taxonomic units	1744:1770	operational taxonomic units (OTUs) significantly higher than control treatment	1744:1821	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	3	95	dep	prebiotic	534:542	arg1	%					526:526	0.5%	523:526	0.5%	523:526	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	1	96	dep	performance	204:214	arg1	the					193:195	the	193:195	the	193:195	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	1	97	theme	intestinal	220:229	arg1	composition					242:252	intestinal microbiota composition	220:252	intestinal microbiota composition	220:252	This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment.					
32683466	3	98	dep	RfR	427:429	arg1	probiotic					431:439	probiotic	431:439	probiotic	431:439	NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probiotic and 0.5% (v/w) prebiotic), or control (without addition of probiotic and prebiotic).					
32683466	9	99	theme	units	1766:1770	arg1	OTU					1856:1858	470, 480, 451, and 344 OTU	1833:1858	470, 480, 451, and 344 OTU	1833:1858	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	9	99	theme	units	1766:1770	arg1	number					1734:1739	a number	1732:1739	a number	1732:1739	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
32683466	9	100	theme	prebiotic	1688:1696	arg1	treatments					1713:1722	The probiotic, prebiotic, and synbiotic treatments	1673:1722	The probiotic, prebiotic, and synbiotic treatments	1673:1722	The probiotic, prebiotic, and synbiotic treatments induced a number of operational taxonomic units (OTUs) significantly higher than control treatment, that is, 470, 480, 451, and 344 OTU, respectively.					
34756437	10	0	theme	yield	1763:1767	arg1	values					1776:1781	yield stress values	1763:1781	yield stress values	1763:1781	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	3	1	theme	gel	651:653	arg1	strength					655:662	increased gel strength	641:662	increased gel strength in cultured products	641:683	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	13	2	theme	bottle	2344:2349	arg1	shaking					2329:2335	gentle shaking	2322:2335	gentle shaking of the bottle at 6 mo	2322:2357	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	3	3	theme	cultured	485:492	arg1	beverages					499:507	cultured milk beverages	485:507	cultured milk beverages	485:507	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	6	4	theme	loss	1094:1097	arg1	tangent					1099:1105	loss tangent	1094:1105	loss tangent	1094:1105	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	2	5	theme	phase	289:293	arg1	separation					295:304	rapid phase separation	283:304	rapid phase separation	283:304	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	4	6	theme	HM	796:797	arg1	pectin					799:804	HM pectin	796:804	HM pectin	796:804	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	13	7	theme	gentle	2322:2327	arg1	shaking					2329:2335	gentle shaking	2322:2335	gentle shaking of the bottle at 6 mo	2322:2357	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	2	8	theme	methoxy	432:438	arg1	pectin					445:450	high methoxy (HM) pectin	427:450	high methoxy (HM) pectin	427:450	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	13	9	contain	have	2264:2267	arg2	values					2286:2291	low yield stress values	2269:2291	low yield stress values	2269:2291	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	13	9	contain	have	2264:2267	arg1	Samples					2213:2219	Samples	2213:2219	Samples in this optimized region	2213:2244	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	9	10	theme	samples	1570:1576	arg1	modulus					1554:1560	elastic modulus	1546:1560	elastic modulus	1546:1560	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	9	10	theme	samples	1570:1576	arg1	stiffness					1535:1543	The stiffness	1531:1543	The stiffness (elastic modulus) of all samples	1531:1576	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	13	11	theme	yield	2273:2277	arg1	values					2286:2291	low yield stress values	2269:2291	low yield stress values	2269:2291	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	10	12	theme	<7.5	1827:1830	arg1	%					1831:1831	%	1831:1831	%	1831:1831	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	11	13	theme	Prediction	1887:1896	arg1	models					1898:1903	Prediction models	1887:1903	Prediction models for visual phase separation in beverages stored for 6 mo	1887:1960	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	1	14	theme	protein	191:197	arg1	levels					199:204	high protein levels	186:204	high protein levels	186:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	11	15	theme	visual	1909:1914	arg1	separation					1922:1931	visual phase separation	1909:1931	visual phase separation in beverages stored for 6 mo	1909:1960	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	4	16	from	temperature	876:886	arg1	storage					857:863	storage	857:863	storage at ambient temperature for 6 mo	857:895	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	6	17	theme	beverages	1134:1142	arg1	modulus					1085:1091	The elastic modulus	1073:1091	The elastic modulus	1073:1091	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	6	17	theme	beverages	1134:1142	arg1	tangent					1099:1105	loss tangent	1094:1105	loss tangent	1094:1105	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	6	17	theme	beverages	1134:1142	arg1	stress					1118:1123	yield stress	1112:1123	yield stress	1112:1123	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	11	18	theme	protein	1997:2003	arg1	content					2005:2011	the protein content	1993:2011	the protein content	1993:2011	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	3	19	from	strength	655:662	arg1	products					676:683	cultured products	667:683	cultured products	667:683	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	3	20	theme	due	580:582	arg1	milk					574:577	milk	574:577	milk	574:577	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	13	21	theme	stress	2279:2284	arg1	values					2286:2291	low yield stress values	2269:2291	low yield stress values	2269:2291	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	6	22	theme	restructuring	1203:1215	arg1	extent					1188:1193	the extent	1184:1193	the extent of bond restructuring	1184:1215	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	5	23	theme	protein	984:990	arg1	contents					1003:1010	the protein and pectin contents	980:1010	contents	1003:1010	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	3	24	dep	investigated	512:523	arg1	limit					456:460	limit	456:460	To limit protein aggregation in cultured milk beverages	453:507	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	6	25	theme	yield	1112:1116	arg1	stress					1118:1123	yield stress	1112:1123	yield stress	1112:1123	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	10	26	theme	≥0.85	1872:1876	arg1	%					1877:1877	%	1877:1877	%	1877:1877	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	5	27	theme	pectin	996:1001	arg1	contents					1003:1010	the protein and pectin contents	980:1010	contents	1003:1010	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	2	28	theme	protein	268:274	arg1	level					276:280	protein level	268:280	protein level	268:280	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	9	29	theme	protein	1640:1646	arg1	content					1648:1654	the protein content	1636:1654	the protein content	1636:1654	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	5	30	theme	0.0	1045:1047	arg1	%					1056:1056	0.0 and 1.0%	1045:1056	%	1056:1056	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	4	31	with	blended	783:789	arg1	pectin					799:804	HM pectin	796:804	HM pectin	796:804	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	11	32	theme	protein	2051:2057	arg1	levels					2059:2064	lower protein levels	2045:2064	lower protein levels	2045:2064	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	5	33	theme	1.0	1053:1055	arg1	%					1056:1056	0.0 and 1.0%	1045:1056	%	1056:1056	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	0	34	from	Effects	0:6	arg1	rheology					59:66	the rheology	55:66	the rheology of high-protein cultured milk beverages	55:106	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	0	35	theme	cultured	84:91	arg1	beverages					98:106	high-protein cultured milk beverages	71:106	high-protein cultured milk beverages	71:106	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	5	36	theme	response	908:915	arg1	methodology					925:935	response surface methodology	908:935	response surface methodology	908:935	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	12	37	theme	optimal	2096:2102	arg1	protein					2104:2110	optimal protein	2096:2110	optimal protein (<7.5%)	2096:2118	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	12	37	theme	optimal	2096:2102	arg1	%					2117:2117	<7.5%	2113:2117	<7.5%	2113:2117	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	0	38	theme	beverages	98:106	arg1	rheology					59:66	the rheology	55:66	the rheology of high-protein cultured milk beverages	55:106	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	9	39	theme	elastic	1680:1686	arg1	bonds					1688:1692	elastic bonds	1680:1692	elastic bonds	1680:1692	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	12	40	used	used	2079:2082	arg2	Models					2067:2072	Models	2067:2072	Models	2067:2072	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	2	41	theme	high	427:430	arg1	methoxy					432:438	high methoxy	427:438	high methoxy (HM) pectin	427:450	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	2	41	theme	high	427:430	arg1	HM					441:442	HM	441:442	HM	441:442	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	13	42	from	Samples	2213:2219	arg1	region					2239:2244	this optimized region	2224:2244	this optimized region	2224:2244	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	0	43	theme	varying	11:17	arg1	concentrations					37:50	varying casein and pectin concentrations	11:50	varying casein and pectin concentrations	11:50	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	1	44	theme	Shelf-stable	139:150	arg1	beverages					166:174	Shelf-stable cultured milk beverages	139:174	Shelf-stable cultured milk beverages that have high protein levels	139:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	7	45	dep	processing	1365:1374	arg1	beverages					1380:1388	the beverages	1376:1388	thermally processing the beverages	1355:1388	Response variables were measured initially after thermally processing the beverages, and after 1 and 6 mo of storage at ambient temperature.					
34756437	3	46	theme	proteins	607:614	arg1	absence					591:597	the absence	587:597	the absence of whey proteins	587:614	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	3	47	theme	protein	462:468	arg1	aggregation					470:480	protein aggregation	462:480	protein aggregation in cultured milk beverages	462:507	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	0	48	theme	concentrations	37:50	arg1	Effects					0:6	Effects	0:6	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages	0:106	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	3	49	theme	whey	602:605	arg1	proteins					607:614	whey proteins	602:614	whey proteins	602:614	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	3	50	theme	increased	641:649	arg1	strength					655:662	increased gel strength	641:662	increased gel strength in cultured products	641:683	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	2	51	theme	cultured	333:340	arg1	beverages					342:350	cultured beverages	333:350	cultured beverages	333:350	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	1	52	theme	cultured	152:159	arg1	beverages					166:174	Shelf-stable cultured milk beverages	139:174	Shelf-stable cultured milk beverages that have high protein levels	139:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	9	53	theme	pectin	1735:1740	arg1	levels					1725:1730	high levels	1720:1730	high levels of pectin	1720:1740	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	12	54	theme	ambient	2196:2202	arg1	storage					2204:2210	ambient storage	2196:2210	ambient storage	2196:2210	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	3	55	theme	milk	494:497	arg1	beverages					499:507	cultured milk beverages	485:507	cultured milk beverages	485:507	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	5	56	theme	composite	952:960	arg1	design					972:977	a central composite rotatable design	942:977	a central composite rotatable design	942:977	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	3	57	theme	cultured	667:674	arg1	products					676:683	cultured products	667:683	cultured products	667:683	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	13	58	from	mo	2356:2357	arg1	shaking					2329:2335	gentle shaking	2322:2335	gentle shaking of the bottle at 6 mo	2322:2357	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	9	59	theme	elastic	1546:1552	arg1	modulus					1554:1560	elastic modulus	1546:1560	elastic modulus	1546:1560	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	9	59	theme	elastic	1546:1552	arg1	stiffness					1535:1543	The stiffness	1531:1543	The stiffness (elastic modulus) of all samples	1531:1576	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	10	60	theme	storage	1754:1760	arg1	mo					1748:1749	6 mo	1746:1749	6 mo of storage	1746:1760	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	3	61	theme	micellar	525:532	arg1	casein					534:539	micellar casein	525:539	micellar casein	525:539	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	3	61	theme	micellar	525:532	arg1	alternative					559:569	an interesting alternative	544:569	an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products	544:683	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	12	62	theme	gels	2184:2187	arg1	stiffness					2171:2179	the stiffness	2167:2179	the stiffness of gels	2167:2187	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	2	63	theme	rapid	283:287	arg1	separation					295:304	rapid phase separation	283:304	rapid phase separation	283:304	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	6	64	theme	elastic	1077:1083	arg1	modulus					1085:1091	The elastic modulus	1073:1091	The elastic modulus	1073:1091	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	3	65	theme	interesting	547:557	arg1	casein					534:539	micellar casein	525:539	micellar casein	525:539	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	3	65	theme	interesting	547:557	arg1	alternative					559:569	an interesting alternative	544:569	an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products	544:683	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	1	66	theme	high	186:189	arg1	levels					199:204	high protein levels	186:204	high protein levels	186:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	0	67	theme	ambient	118:124	arg1	temperature					126:136	ambient temperature	118:136	ambient temperature	118:136	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	3	68	from	aggregation	470:480	arg1	beverages					499:507	cultured milk beverages	485:507	cultured milk beverages	485:507	To limit protein aggregation in cultured milk beverages we investigated micellar casein as an interesting alternative to milk, due to the absence of whey proteins, which can contribute to increased gel strength in cultured products.					
34756437	2	69	theme	gel	310:312	arg1	formation					314:322	gel formation	310:322	gel formation	310:322	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	11	70	theme	phase	1916:1920	arg1	separation					1922:1931	visual phase separation	1909:1931	visual phase separation in beverages stored for 6 mo	1909:1960	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	7	71	theme	Response	1306:1313	arg1	variables					1315:1323	Response variables	1306:1323	Response variables	1306:1323	Response variables were measured initially after thermally processing the beverages, and after 1 and 6 mo of storage at ambient temperature.					
34756437	6	72	theme	particle	1226:1233	arg1	size					1235:1238	particle size	1226:1238	particle size	1226:1238	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	4	73	theme	ambient	868:874	arg1	temperature					876:886	ambient temperature	868:886	ambient temperature for 6 mo	868:895	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	13	74	theme	low	2269:2271	arg1	values					2286:2291	low yield stress values	2269:2291	low yield stress values	2269:2291	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	5	75	theme	5	1032:1032	arg1	%					1039:1039	5 and 9%	1032:1039	%	1039:1039	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	10	76	theme	%	1831:1831	arg1	protein					1833:1839	<7.5% protein	1827:1839	<7.5% protein	1827:1839	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	6	77	theme	bond	1198:1201	arg1	restructuring					1203:1215	bond restructuring	1198:1215	bond restructuring	1198:1215	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	4	78	from	fermented	762:770	arg1	study					694:698	this study	689:698	this study	689:698	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	4	79	dep	milk	744:747	arg1	permeate					749:756	permeate	749:756	permeate	749:756	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	0	80	theme	high-protein	71:82	arg1	beverages					98:106	high-protein cultured milk beverages	71:106	high-protein cultured milk beverages	71:106	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	1	81	theme	milk	161:164	arg1	beverages					166:174	Shelf-stable cultured milk beverages	139:174	Shelf-stable cultured milk beverages that have high protein levels	139:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	12	82	theme	pectin	2124:2129	arg1	concentrations					2140:2153	optimal protein (<7.5%) and pectin (≥0.85%) concentrations	2096:2153	optimal protein (<7.5%) and pectin (≥0.85%) concentrations	2096:2153	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	0	83	theme	milk	93:96	arg1	beverages					98:106	high-protein cultured milk beverages	71:106	high-protein cultured milk beverages	71:106	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	12	84	theme	protein	2104:2110	arg1	concentrations					2140:2153	optimal protein (<7.5%) and pectin (≥0.85%) concentrations	2096:2153	optimal protein (<7.5%) and pectin (≥0.85%) concentrations	2096:2153	Models were used to identify optimal protein (<7.5%) and pectin (≥0.85%) concentrations to minimize the stiffness of gels during ambient storage.					
34756437	10	85	theme	%	1877:1877	arg1	pectin					1879:1884	≥0.85% pectin	1872:1884	≥0.85% pectin	1872:1884	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	6	86	theme	phase	1251:1255	arg1	separation					1257:1266	visual phase separation	1244:1266	visual phase separation	1244:1266	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	5	87	theme	9	1038:1038	arg1	%					1039:1039	5 and 9%	1032:1039	%	1039:1039	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	5	88	theme	surface	917:923	arg1	methodology					925:935	response surface methodology	908:935	response surface methodology	908:935	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	4	89	theme	ultrafiltered	730:742	arg1	milk					744:747	ultrafiltered milk	730:747	ultrafiltered milk permeate	730:756	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	9	90	theme	bonds	1688:1692	arg1	growth					1670:1675	the growth	1666:1675	the growth of elastic bonds over time	1666:1702	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	0	91	theme	casein	19:24	arg1	concentrations					37:50	varying casein and pectin concentrations	11:50	varying casein and pectin concentrations	11:50	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	4	92	from	study	694:698	arg1	fermented					762:770	fermented	762:770	fermented	762:770	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	13	93	theme	optimized	2229:2237	arg1	region					2239:2244	this optimized region	2224:2244	this optimized region	2224:2244	Samples in this optimized region were predicted to have low yield stress values and were easily fluidized by gentle shaking of the bottle at 6 mo.					
34756437	10	94	with	beverages	1812:1820	arg1	protein					1833:1839	<7.5% protein	1827:1839	<7.5% protein	1827:1839	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
34756437	4	95	theme	micellar	701:708	arg1	casein					710:715	micellar casein	701:715	micellar casein dispersed in ultrafiltered milk permeate	701:756	In this study, micellar casein dispersed in ultrafiltered milk permeate was fermented to pH 4.1, blended with HM pectin, homogenized, thermally processed, and bottled for storage at ambient temperature for 6 mo.					
34756437	2	96	theme	stabilizers	407:417	arg1	inclusion					394:402	the inclusion	390:402	the inclusion of stabilizers such as high methoxy (HM) pectin	390:450	With increasing protein level, rapid phase separation and gel formation occur in cultured beverages, which may not be prevented even with the inclusion of stabilizers such as high methoxy (HM) pectin.					
34756437	0	97	theme	pectin	30:35	arg1	concentrations					37:50	varying casein and pectin concentrations	11:50	varying casein and pectin concentrations	11:50	Effects of varying casein and pectin concentrations on the rheology of high-protein cultured milk beverages stored at ambient temperature.					
34756437	5	98	theme	rotatable	962:970	arg1	design					972:977	a central composite rotatable design	942:977	a central composite rotatable design	942:977	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	11	99	theme	lower	2045:2049	arg1	levels					2059:2064	lower protein levels	2045:2064	lower protein levels	2045:2064	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	1	100	contain	have	181:184	arg2	levels					199:204	high protein levels	186:204	high protein levels	186:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	1	100	contain	have	181:184	arg1	beverages					166:174	Shelf-stable cultured milk beverages	139:174	Shelf-stable cultured milk beverages that have high protein levels	139:204	Shelf-stable cultured milk beverages that have high protein levels can be difficult to successfully manufacture.					
34756437	6	101	theme	visual	1244:1249	arg1	separation					1257:1266	visual phase separation	1244:1266	visual phase separation	1244:1266	The elastic modulus, loss tangent, and yield stress of these beverages were measured during storage to observe the extent of bond restructuring, whereas particle size and visual phase separation were measured to determine stability.					
34756437	11	102	from	separation	1922:1931	arg1	beverages					1936:1944	beverages	1936:1944	beverages stored for 6 mo	1936:1960	Prediction models for visual phase separation in beverages stored for 6 mo were significantly affected by the protein content, with increasing instability at lower protein levels.					
34756437	7	103	theme	ambient	1426:1432	arg1	temperature					1434:1444	ambient temperature	1426:1444	ambient temperature	1426:1444	Response variables were measured initially after thermally processing the beverages, and after 1 and 6 mo of storage at ambient temperature.					
34756437	9	104	theme	high	1720:1723	arg1	levels					1725:1730	high levels	1720:1730	high levels of pectin	1720:1740	The stiffness (elastic modulus) of all samples increased throughout storage and was determined mainly by the protein content; however, the growth of elastic bonds over time was slowed with high levels of pectin.					
34756437	7	105	theme	storage	1415:1421	arg1	mo					1409:1410	1 and 6 mo	1401:1410	1 and 6 mo of storage	1401:1421	Response variables were measured initially after thermally processing the beverages, and after 1 and 6 mo of storage at ambient temperature.					
34756437	5	106	theme	central	944:950	arg1	design					972:977	a central composite rotatable design	942:977	a central composite rotatable design	942:977	Utilizing response surface methodology with a central composite rotatable design, the protein and pectin contents were varied between 5 and 9% and 0.0 and 1.0%, respectively.					
34756437	8	107	theme	pectin	1517:1522	arg1	level					1524:1528	the pectin level	1513:1528	the pectin level	1513:1528	All samples quickly formed gels after homogenizing, regardless of the pectin level.					
34756437	10	108	theme	stress	1769:1774	arg1	values					1776:1781	yield stress values	1763:1781	yield stress values	1763:1781	At 6 mo of storage, yield stress values were significantly lower for beverages with <7.5% protein when they were stabilized with ≥0.85% pectin.					
32004608	4	0	mod	modification	543:554	arg1	CNFs					559:562	CNFs	559:562	CNFs	559:562	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	0	mod	modification	543:554	arg3	surface					535:541	the surface modification	531:554	the surface modification of CNFs	531:562	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	1	dep	compare	509:515	arg1	to					506:507	to	506:507	to	506:507	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	3	2	theme	esterification	471:484	arg1	processes					486:494	two different esterification processes	457:494	two different esterification processes	457:494	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	7	3	theme	thermal	1347:1353	arg1	properties					1355:1364	mechanical and thermal properties	1332:1364	mechanical and thermal properties	1332:1364	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	7	3	theme	thermal	1347:1353	arg1	transmittance					1317:1329	the crystallinity, light transmittance	1292:1329	transmittance	1317:1329	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	4	4	theme	tensile	823:829	arg1	tester					831:836	tensile tester	823:836	tensile tester	823:836	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	5	theme	modification	543:554	arg1	effect					521:526	the effect	517:526	the effect of the surface modification of CNFs	517:562	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	7	6	theme	light	1311:1315	arg1	properties					1355:1364	mechanical and thermal properties	1332:1364	mechanical and thermal properties	1332:1364	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	7	6	theme	light	1311:1315	arg1	transmittance					1317:1329	the crystallinity, light transmittance	1292:1329	transmittance	1317:1329	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	4	7	theme	X-ray	740:744	arg1	XRD					759:761	XRD	759:761	XRD	759:761	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	7	theme	X-ray	740:744	arg1	diffraction					746:756	X-ray diffraction	740:756	X-ray diffraction (XRD)	740:762	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	8	theme	solvent	637:643	arg1	method					657:662	solvent evaporation method	637:662	solvent evaporation method	637:662	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	2	9	with	structure	282:290	arg1	lengths					342:348	lengths	342:348	lengths reaching a few microns	342:371	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	2	9	with	structure	282:290	arg1	diameters					297:305	diameters	297:305	diameters ranging between 2 nm and 27 nm	297:336	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	5	10	theme	successful	889:898	arg1	grafting					900:907	a successful grafting	887:907	a successful grafting of the long chain hydrocarbon structure onto the CNFs	887:961	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	5	11	theme	chain	921:925	arg1	structure					939:947	the long chain hydrocarbon structure	912:947	the long chain hydrocarbon structure onto the CNFs	912:961	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	2	12	theme	mechanical	245:254	arg1	treatments					256:265	chemical and mechanical treatments	232:265	chemical and mechanical treatments	232:265	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	4	13	theme	CNFs	559:562	arg1	modification					543:554	the surface modification	531:554	the surface modification of CNFs	531:562	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	14	theme	UV-vis	799:804	arg1	spectroscopy					806:817	UV-vis spectroscopy	799:817	UV-vis spectroscopy	799:817	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	5	15	theme	hydrocarbon	927:937	arg1	structure					939:947	the long chain hydrocarbon structure	912:947	the long chain hydrocarbon structure onto the CNFs	912:961	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	2	16	theme	few	361:363	arg1	microns					365:371	a few microns	359:371	a few microns	359:371	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	2	17	theme	chemical	232:239	arg1	treatments					256:265	chemical and mechanical treatments	232:265	chemical and mechanical treatments	232:265	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	4	18	theme	FTIR	720:723	arg1	spectroscopy					726:737	(FTIR) spectroscopy	719:737	(FTIR) spectroscopy	719:737	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	7	19	theme	high	1245:1248	arg1	degree					1250:1255	high degree	1245:1255	high degree of substitution	1245:1271	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	7	20	theme	crystallinity	1296:1308	arg1	properties					1355:1364	mechanical and thermal properties	1332:1364	mechanical and thermal properties	1332:1364	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	7	20	theme	crystallinity	1296:1308	arg1	transmittance					1317:1329	the crystallinity, light transmittance	1292:1329	transmittance	1317:1329	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	0	21	theme	Esterified	0:9	arg1	cellulose					11:19	Esterified cellulose	0:19	Esterified cellulose	0:19	Esterified cellulose nanofibres from saw dust using vegetable oil.					
32004608	5	22	theme	unmodified	1009:1018	arg1	nanopapers					1031:1040	unmodified CNFs-based nanopapers	1009:1040	unmodified CNFs-based nanopapers	1009:1040	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	2	23	theme	web-like	273:280	arg1	structure					282:290	a web-like structure	271:290	a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns	271:371	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	1	24	theme	underutilized	150:162	arg1	sawdust					129:135	sawdust	129:135	sawdust	129:135	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	1	24	theme	underutilized	150:162	arg1	by-product					164:173	an underutilized by-product	147:173	an underutilized by-product from the wood and timber industry	147:207	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	4	25	theme	thermogravimetric	765:781	arg1	TGA					793:795	TGA	793:795	TGA	793:795	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	25	theme	thermogravimetric	765:781	arg1	analysis					783:790	thermogravimetric analysis	765:790	thermogravimetric analysis (TGA)	765:796	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	1	26	theme	cellulose	81:89	arg1	CNFs					103:106	CNFs	103:106	CNFs	103:106	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	1	26	theme	cellulose	81:89	arg1	nanofibres					91:100	cellulose nanofibres	81:100	cellulose nanofibres (CNFs)	81:107	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	3	27	theme	vegetable	430:438	arg1	oil					447:449	vegetable canola oil	430:449	vegetable canola oil using two different esterification processes	430:494	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	4	28	dep	transform	700:708	arg1	infrared					710:717	infrared	710:717	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester	700:836	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	4	29	theme	surface	535:541	arg1	modification					543:554	the surface modification	531:554	the surface modification of CNFs	531:562	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	5	30	theme	CNFs-based	1020:1029	arg1	nanopapers					1031:1040	unmodified CNFs-based nanopapers	1009:1040	unmodified CNFs-based nanopapers	1009:1040	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	3	31	theme	canola	440:445	arg1	oil					447:449	vegetable canola oil	430:449	vegetable canola oil using two different esterification processes	430:494	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	3	32	theme	different	461:469	arg1	processes					486:494	two different esterification processes	457:494	two different esterification processes	457:494	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	6	33	theme	substitution	1213:1224	arg1	degree					1203:1208	the degree	1199:1208	the degree of substitution	1199:1224	The crystallinity, mechanical, light transmittance and thermal properties were significantly affected primarily by the esterification method employed, thus the degree of substitution.					
32004608	4	34	theme	evaporation	645:655	arg1	method					657:662	solvent evaporation method	637:662	solvent evaporation method	637:662	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	7	35	theme	substitution	1260:1271	arg1	degree					1250:1255	high degree	1245:1255	high degree of substitution	1245:1271	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	0	36	theme	saw	37:39	arg1	dust					41:44	saw dust	37:44	saw dust using vegetable oil	37:64	Esterified cellulose nanofibres from saw dust using vegetable oil.					
32004608	2	37	contain	had	267:269	arg2	structure					282:290	a web-like structure	271:290	a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns	271:371	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	2	37	contain	had	267:269	arg1	CNFs					224:227	The extracted CNFs	210:227	The extracted CNFs by chemical and mechanical treatments	210:265	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	3	38	theme	obtained	378:385	arg1	CNFs					387:390	The obtained CNFs	374:390	The obtained CNFs	374:390	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	8	39	theme	substation	1431:1440	arg1	0.8					1452:1454	0.8	1452:1454	0.8	1452:1454	The crystallinity decreased from 70% to <40% when the degree of substation was about 0.8.					
32004608	8	39	theme	substation	1431:1440	arg1	degree					1421:1426	the degree	1417:1426	the degree of substation	1417:1440	The crystallinity decreased from 70% to <40% when the degree of substation was about 0.8.					
32004608	1	40	theme	wood	184:187	arg1	industry					200:207	the wood and timber industry	180:207	industry	200:207	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	0	41	theme	vegetable	52:60	arg1	oil					62:64	vegetable oil	52:64	vegetable oil	52:64	Esterified cellulose nanofibres from saw dust using vegetable oil.					
32004608	1	42	from	industry	200:207	arg1	sawdust					129:135	sawdust	129:135	sawdust	129:135	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	1	42	from	industry	200:207	arg1	by-product					164:173	an underutilized by-product	147:173	an underutilized by-product from the wood and timber industry	147:207	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	6	43	theme	thermal	1098:1104	arg1	properties					1106:1115	thermal properties	1098:1115	thermal properties	1098:1115	The crystallinity, mechanical, light transmittance and thermal properties were significantly affected primarily by the esterification method employed, thus the degree of substitution.					
32004608	8	44	theme	70	1400:1401	arg1	%					1402:1402	%	1402:1402	%	1402:1402	The crystallinity decreased from 70% to <40% when the degree of substation was about 0.8.					
32004608	3	45	mod	modified	416:423	arg3	oil					447:449	vegetable canola oil	430:449	vegetable canola oil using two different esterification processes	430:494	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	3	45	mod	modified	416:423	arg1	CNFs					387:390	The obtained CNFs	374:390	The obtained CNFs	374:390	The obtained CNFs were further chemically modified with vegetable canola oil using two different esterification processes.					
32004608	5	46	theme	FTIR	839:842	arg1	results					844:850	FTIR results	839:850	FTIR results	839:850	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	8	47	theme	<40	1407:1409	arg1	%					1402:1402	%	1402:1402	%	1402:1402	The crystallinity decreased from 70% to <40% when the degree of substation was about 0.8.					
32004608	4	48	dep	Fourier	692:698	arg1	transform					700:708	transform	700:708	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester	700:836	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	5	49	theme	structure	939:947	arg1	grafting					900:907	a successful grafting	887:907	a successful grafting of the long chain hydrocarbon structure onto the CNFs	887:961	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	5	50	theme	long	916:919	arg1	structure					939:947	the long chain hydrocarbon structure	912:947	the long chain hydrocarbon structure onto the CNFs	912:961	FTIR results indicated that both methods led to a successful grafting of the long chain hydrocarbon structure onto the CNFs, and became more hydrophobic when compared to unmodified CNFs-based nanopapers.					
32004608	6	51	theme	light	1074:1078	arg1	transmittance					1080:1092	light transmittance	1074:1092	light transmittance	1074:1092	The crystallinity, mechanical, light transmittance and thermal properties were significantly affected primarily by the esterification method employed, thus the degree of substitution.					
32004608	1	52	theme	timber	193:198	arg1	industry					200:207	the wood and timber industry	180:207	industry	200:207	In this work, cellulose nanofibres (CNFs) were extracted from sawdust, which is an underutilized by-product from the wood and timber industry.					
32004608	4	53	theme	prospective	605:615	arg1	suspensions					617:627	their prospective suspensions	599:627	their prospective suspensions	599:627	In order to compare the effect of the surface modification of CNFs, the nanopapers were prepared from their prospective suspensions through solvent evaporation method, and then characterize with Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-vis spectroscopy and tensile tester.					
32004608	2	54	theme	extracted	214:222	arg1	CNFs					224:227	The extracted CNFs	210:227	The extracted CNFs by chemical and mechanical treatments	210:265	The extracted CNFs by chemical and mechanical treatments had a web-like structure with diameters ranging between 2 nm and 27 nm and lengths reaching a few microns.					
32004608	8	55	dep	%	1402:1402	arg1	to					1404:1405	to	1404:1405	to	1404:1405	The crystallinity decreased from 70% to <40% when the degree of substation was about 0.8.					
32004608	7	56	theme	mechanical	1332:1341	arg1	properties					1355:1364	mechanical and thermal properties	1332:1364	mechanical and thermal properties	1332:1364	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	7	56	theme	mechanical	1332:1341	arg1	transmittance					1317:1329	the crystallinity, light transmittance	1292:1329	transmittance	1317:1329	It was found that high degree of substitution adversely affected the crystallinity, light transmittance, mechanical and thermal properties.					
32004608	6	57	theme	esterification	1162:1175	arg1	method					1177:1182	the esterification method	1158:1182	the esterification method employed	1158:1191	The crystallinity, mechanical, light transmittance and thermal properties were significantly affected primarily by the esterification method employed, thus the degree of substitution.					
34390325	9	0	theme	translational	1883:1895	arg1	platform					1897:1904	a refinable and translational platform	1867:1904	a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI	1867:1988	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	2	1	theme	Conductive	366:375	arg1	biomaterials					377:388	Conductive biomaterials	366:388	Conductive biomaterials	366:388	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	2	dep	scaffolds	817:825	arg1	3					770:770	3	770:770	3	770:770	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	9	3	theme	synergistic	1917:1927	arg1	combination					1929:1939	future synergistic combination	1910:1939	future synergistic combination with ES	1910:1947	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	0	4	theme	Conductive	0:9	arg1	nanorod					55:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.					
34390325	2	5	theme	developing	636:645	arg1	biomaterials					658:669	developing conductive biomaterials	636:669	developing conductive biomaterials	636:669	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	1	6	theme	medicine	217:224	arg1	fields					226:231	the rehabilitation and regenerative medicine fields	181:231	the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI)	181:294	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	6	7	theme	nontoxic	1322:1329	arg1	citrate-GNRs					1331:1342	nontoxic citrate-GNRs	1322:1342	nontoxic citrate-GNRs	1322:1342	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	8	8	theme	rat	1742:1744	arg1	cells					1758:1762	seeded rat neural stem cells	1735:1762	seeded rat neural stem cells	1735:1762	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	2	9	theme	better	563:568	arg1	recovery					581:588	better functional recovery	563:588	better functional recovery	563:588	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	9	10	theme	functional	1960:1969	arg1	recovery					1971:1978	functional recovery	1960:1978	functional recovery	1960:1978	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	3	11	theme	Pre-formed	851:860	arg1	scaffolds					877:885	Pre-formed, noninjectable scaffolds	851:885	Pre-formed, noninjectable scaffolds	851:885	Pre-formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion-type of SCI.					
34390325	6	12	theme	indirect	1412:1419	arg1	exchange					1428:1435	an indirect ligand exchange	1409:1435	an indirect ligand exchange	1409:1435	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	8	13	theme	stem	1753:1756	arg1	cells					1758:1762	seeded rat neural stem cells	1735:1762	seeded rat neural stem cells	1735:1762	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	1	14	theme	functional	245:254	arg1	recovery					256:263	functional recovery	245:263	functional recovery after spinal cord injury (SCI)	245:294	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	2	15	dep	challenges	622:631	arg1	conductivity					680:691	(1) low conductivity	672:691	three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites	610:716	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	16	dep	conductivity	680:691	arg1	1					673:673	1	673:673	1	673:673	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	17	theme	composites	707:716	arg1	conductivity					680:691	(1) low conductivity	672:691	three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites	610:716	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	18	theme	conductive	728:737	arg1	components					739:748	many conductive components	723:748	many conductive components	723:748	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	6	19	theme	aspect	1373:1378	arg1	GNRs					1386:1389	high aspect ratio GNRs	1368:1389	high aspect ratio GNRs	1368:1389	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	2	20	theme	neurons	503:509	arg1	ES					497:498	direct ES	490:498	direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery	490:588	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	4	21	from	bioinks	1051:1057	arg1	lessons					1038:1044	lessons	1038:1044	lessons from bioinks in the bioprinting field	1038:1082	Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs.					
34390325	4	21	from	bioinks	1051:1057	arg1	field					1078:1082	the bioprinting field	1062:1082	the bioprinting field	1062:1082	Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs.					
34390325	6	22	theme	robust	1347:1352	arg1	synthesis					1354:1362	a robust synthesis	1345:1362	a robust synthesis for high aspect ratio GNRs	1345:1389	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	3	23	dep	Pre-formed	851:860	arg1	noninjectable					863:875	noninjectable	863:875	noninjectable	863:875	Pre-formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion-type of SCI.					
34390325	2	24	dep	enable	394:399	arg1	enable					483:488	enable	483:488	may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery	479:588	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	25	theme	biomaterials	428:439	arg1	combination					413:423	synergistic combination	401:423	synergistic combination of biomaterials with electric stimulation (ES)	401:470	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	3	26	theme	clinical	898:905	arg1	translation					907:917	clinical translation	898:917	clinical translation	898:917	Pre-formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion-type of SCI.					
34390325	0	27	theme	surgical	86:93	arg1	translation					95:105	enhanced surgical translation	77:105	enhanced surgical translation	77:105	Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.					
34390325	6	28	theme	ligand	1421:1426	arg1	exchange					1428:1435	an indirect ligand exchange	1409:1435	an indirect ligand exchange	1409:1435	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	2	29	theme	many	723:726	arg1	components					739:748	many conductive components	723:748	many conductive components	723:748	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	30	theme	low	676:678	arg1	conductivity					680:691	(1) low conductivity	672:691	three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites	610:716	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	5	31	theme	gelatin	1291:1297	arg1	hydrogel					1299:1306	gelatin hydrogel	1291:1306	gelatin hydrogel	1291:1306	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	0	32	theme	injectable	15:24	arg1	nanorod					55:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.					
34390325	5	33	theme	conductive	1166:1175	arg1	hydrogel					1177:1184	a conductive hydrogel	1164:1184	a conductive hydrogel	1164:1184	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	3	34	theme	surgical	924:931	arg1	context					933:939	a surgical context	922:939	a surgical context for the most common contusion-type of SCI	922:981	Pre-formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion-type of SCI.					
34390325	4	35	theme	injectable	1002:1011	arg1	biomaterial					1013:1023	an injectable biomaterial	999:1023	an injectable biomaterial	999:1023	Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs.					
34390325	4	35	theme	injectable	1002:1011	arg1	translational					1097:1109	translational	1097:1109	translational	1097:1109	Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs.					
34390325	2	36	theme	electric	446:453	arg1	ES					468:469	ES	468:469	ES	468:469	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	36	theme	electric	446:453	arg1	stimulation					455:465	electric stimulation	446:465	electric stimulation (ES)	446:470	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	0	37	theme	acid/gelatin/gold	37:53	arg1	nanorod					55:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.					
34390325	1	38	theme	limited	311:317	arg1	methods					319:325	limited methods	311:325	limited methods	311:325	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	2	39	theme	direct	490:495	arg1	ES					497:498	direct ES	490:498	direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery	490:588	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	9	40	theme	conductive	1826:1835	arg1	hydrogel/bioink					1837:1851	a GNR conductive hydrogel/bioink	1820:1851	a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI	1820:1988	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	0	41	theme	enhanced	77:84	arg1	translation					95:105	enhanced surgical translation	77:105	enhanced surgical translation	77:105	Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.					
34390325	8	42	link	crosslinked	1678:1688	arg1	hydrogel					1690:1697	the crosslinked hydrogel	1674:1697	the crosslinked hydrogel	1674:1697	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	5	43	theme	current	1149:1155	arg1	study					1157:1161	the current study	1145:1161	the current study	1145:1161	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	7	44	theme	enhanced	1498:1505	arg1	placement					1516:1524	enhanced surgical placement	1498:1524	enhanced surgical placement	1498:1524	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	2	45	theme	pre-formed	806:815	arg1	biomaterials					789:800	many conductive biomaterials	773:800	many conductive biomaterials	773:800	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	45	theme	pre-formed	806:815	arg1	scaffolds					817:825	pre-formed scaffolds	806:825	pre-formed scaffolds	806:825	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	6	46	theme	nontoxic	1476:1483	arg1	citrate					1485:1491	nontoxic citrate	1476:1491	nontoxic citrate	1476:1491	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	5	47	theme	high	1217:1220	arg1	GNRs					1258:1261	GNRs	1258:1261	GNRs	1258:1261	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	5	47	theme	high	1217:1220	arg1	nanorods					1248:1255	high aspect ratio citrate-gold nanorods	1217:1255	high aspect ratio citrate-gold nanorods (GNRs)	1217:1262	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	2	48	dep	cytotoxic	754:762	arg1	2					720:720	2	720:720	2	720:720	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	5	49	theme	ratio	1229:1233	arg1	GNRs					1258:1261	GNRs	1258:1261	GNRs	1258:1261	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	5	49	theme	ratio	1229:1233	arg1	nanorods					1248:1255	high aspect ratio citrate-gold nanorods	1217:1255	high aspect ratio citrate-gold nanorods (GNRs)	1217:1262	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	2	50	with	combination	413:423	arg1	ES					468:469	ES	468:469	ES	468:469	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	50	with	combination	413:423	arg1	stimulation					455:465	electric stimulation	446:465	electric stimulation (ES)	446:470	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	3	51	theme	SCI	979:981	arg1	contusion-type					961:974	the most common contusion-type	945:974	the most common contusion-type of SCI	945:981	Pre-formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion-type of SCI.					
34390325	2	52	theme	major	616:620	arg1	challenges					622:631	three major challenges	610:631	three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites	610:716	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	4	53	theme	bioprinting	1066:1076	arg1	field					1078:1082	the bioprinting field	1062:1082	the bioprinting field	1062:1082	Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs.					
34390325	9	54	theme	future	1910:1915	arg1	combination					1929:1939	future synergistic combination	1910:1939	future synergistic combination with ES	1910:1947	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	8	55	theme	crosslinked	1678:1688	arg1	hydrogel					1690:1697	the crosslinked hydrogel	1674:1697	the crosslinked hydrogel	1674:1697	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	1	56	theme	rehabilitation	185:198	arg1	fields					226:231	the rehabilitation and regenerative medicine fields	181:231	the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI)	181:294	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	2	57	theme	conductive	647:656	arg1	biomaterials					658:669	developing conductive biomaterials	636:669	developing conductive biomaterials	636:669	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	1	58	theme	regenerative	204:215	arg1	medicine					217:224	regenerative medicine	204:224	regenerative medicine	204:224	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	2	59	theme	many	773:776	arg1	biomaterials					789:800	many conductive biomaterials	773:800	many conductive biomaterials	773:800	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	59	theme	many	773:776	arg1	scaffolds					817:825	pre-formed scaffolds	806:825	pre-formed scaffolds	806:825	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	60	theme	functional	570:579	arg1	recovery					581:588	better functional recovery	563:588	better functional recovery	563:588	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	4	61	theme	contusion	1115:1123	arg1	SCIs					1125:1128	contusion SCIs	1115:1128	contusion SCIs	1115:1128	Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs.					
34390325	8	62	theme	seeded	1735:1740	arg1	cells					1758:1762	seeded rat neural stem cells	1735:1762	seeded rat neural stem cells	1735:1762	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	8	63	theme	neural	1746:1751	arg1	cells					1758:1762	seeded rat neural stem cells	1735:1762	seeded rat neural stem cells	1735:1762	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	1	64	theme	growing	133:139	arg1	evidence					141:148	growing evidence	133:148	growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI)	133:294	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	8	65	theme	cells	1758:1762	arg1	adhesion/viability					1713:1730	the adhesion/viability	1709:1730	the adhesion/viability of seeded rat neural stem cells	1709:1762	Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro.					
34390325	5	66	theme	hyaluronic	1271:1280	arg1	acid					1282:1285	a hyaluronic acid	1269:1285	a hyaluronic acid	1269:1285	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	6	67	theme	ratio	1380:1384	arg1	GNRs					1386:1389	high aspect ratio GNRs	1368:1389	high aspect ratio GNRs	1368:1389	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	7	68	dep	4 min	1657:1661	arg1	i.e.					1651:1654	i.e.	1651:1654	i.e.	1651:1654	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	6	69	theme	high	1368:1371	arg1	GNRs					1386:1389	high aspect ratio GNRs	1368:1389	high aspect ratio GNRs	1368:1389	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	7	70	theme	precursor	1540:1548	arg1	4 min					1657:1661	4 min	1657:1661	4 min	1657:1661	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	70	theme	precursor	1540:1548	arg1	i.e.					1560:1563	i.e.	1560:1563	i.e.	1560:1563	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	70	theme	precursor	1540:1548	arg1	solution					1550:1557	the hydrogel precursor solution	1527:1557	the hydrogel precursor solution	1527:1557	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	70	theme	precursor	1540:1548	arg1	paste-like					1591:1600	paste-like	1591:1600	paste-like	1591:1600	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	71	dep	paste-like	1591:1600	arg1	4 min					1657:1661	4 min	1657:1661	4 min	1657:1661	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	71	dep	paste-like	1591:1600	arg1	i.e.					1560:1563	i.e.	1560:1563	i.e.	1560:1563	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	71	dep	paste-like	1591:1600	arg1	solution					1550:1557	the hydrogel precursor solution	1527:1557	the hydrogel precursor solution	1527:1557	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	71	dep	paste-like	1591:1600	arg1	paste-like					1591:1600	paste-like	1591:1600	paste-like	1591:1600	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	2	72	theme	axon	522:525	arg1	regeneration					527:538	axon regeneration	522:538	axon regeneration	522:538	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	73	theme	conductive	696:705	arg1	composites					707:716	conductive composites	696:716	conductive composites	696:716	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	74	theme	synergistic	401:411	arg1	combination					413:423	synergistic combination	401:423	synergistic combination of biomaterials with electric stimulation (ES)	401:470	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	0	75	theme	hyaluronic	26:35	arg1	nanorod					55:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod	0:61	Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting.					
34390325	2	76	from	challenges	622:631	arg1	biomaterials					658:669	developing conductive biomaterials	636:669	developing conductive biomaterials	636:669	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	7	77	theme	hydrogel	1531:1538	arg1	4 min					1657:1661	4 min	1657:1661	4 min	1657:1661	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	77	theme	hydrogel	1531:1538	arg1	i.e.					1560:1563	i.e.	1560:1563	i.e.	1560:1563	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	77	theme	hydrogel	1531:1538	arg1	solution					1550:1557	the hydrogel precursor solution	1527:1557	the hydrogel precursor solution	1527:1557	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	7	77	theme	hydrogel	1531:1538	arg1	paste-like					1591:1600	paste-like	1591:1600	paste-like	1591:1600	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	9	78	theme	refinable	1869:1877	arg1	platform					1897:1904	a refinable and translational platform	1867:1904	a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI	1867:1988	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	9	79	theme	GNR	1822:1824	arg1	hydrogel/bioink					1837:1851	a GNR conductive hydrogel/bioink	1820:1851	a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI	1820:1988	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	1	80	theme	spinal	271:276	arg1	SCI					291:293	SCI	291:293	SCI	291:293	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	1	80	theme	spinal	271:276	arg1	injury					283:288	spinal cord injury	271:288	spinal cord injury (SCI)	271:294	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	9	81	theme	current	1778:1784	arg1	study					1786:1790	The current study	1774:1790	The current study	1774:1790	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	2	82	dep	neurons	503:509	arg1	enhance					514:520	enhance	514:520	to enhance axon regeneration and reorganization for better functional recovery	511:588	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	9	83	with	combination	1929:1939	arg1	ES					1946:1947	ES	1946:1947	ES	1946:1947	The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.					
34390325	7	84	theme	surgical	1507:1514	arg1	placement					1516:1524	enhanced surgical placement	1498:1524	enhanced surgical placement	1498:1524	For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste-like, injectable/bioprintable, and fast-crosslinking (i.e., 4 min).					
34390325	3	85	theme	common	954:959	arg1	contusion-type					961:974	the most common contusion-type	945:974	the most common contusion-type of SCI	945:981	Pre-formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion-type of SCI.					
34390325	5	86	theme	aspect	1222:1227	arg1	GNRs					1258:1261	GNRs	1258:1261	GNRs	1258:1261	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	5	86	theme	aspect	1222:1227	arg1	nanorods					1248:1255	high aspect ratio citrate-gold nanorods	1217:1255	high aspect ratio citrate-gold nanorods (GNRs)	1217:1262	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	2	87	theme	conductive	778:787	arg1	biomaterials					789:800	many conductive biomaterials	773:800	many conductive biomaterials	773:800	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	2	87	theme	conductive	778:787	arg1	scaffolds					817:825	pre-formed scaffolds	806:825	pre-formed scaffolds	806:825	Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre-formed scaffolds and are not injectable.					
34390325	1	88	theme	cord	278:281	arg1	SCI					291:293	SCI	291:293	SCI	291:293	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	1	88	theme	cord	278:281	arg1	injury					283:288	spinal cord injury	271:288	spinal cord injury (SCI)	271:294	There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields.					
34390325	6	89	theme	cytotoxic	1451:1459	arg1	surfactant					1461:1470	a cytotoxic surfactant	1449:1470	a cytotoxic surfactant for nontoxic citrate	1449:1491	To fabricate nontoxic citrate-GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate.					
34390325	5	90	theme	citrate-gold	1235:1246	arg1	GNRs					1258:1261	GNRs	1258:1261	GNRs	1258:1261	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
34390325	5	90	theme	citrate-gold	1235:1246	arg1	nanorods					1248:1255	high aspect ratio citrate-gold nanorods	1217:1255	high aspect ratio citrate-gold nanorods (GNRs)	1217:1262	Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate-gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel.					
33249147	7	0	theme	novel	1147:1151	arg1	composites					1153:1162	These novel composites	1141:1162	These novel composites	1141:1162	These novel composites showed promising properties for applications as absorbents.					
33249147	3	1	theme	RH	743:744	arg1	area					727:730	the surface area	715:730	the surface area of treated RH	715:744	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	1	2	theme	starch	201:206	arg1	biocomposites					220:232	thermoplastic cassava starch (TPS) based biocomposites	179:232	thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	179:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	3	theme	husk	284:287	arg1	RH					290:291	RH	290:291	RH	290:291	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	3	theme	husk	284:287	arg1	%					252:252	5-20 wt%	245:252	5-20 wt% of untreated and treated rice husk (RH)	245:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	3	theme	husk	284:287	arg1	husk					284:287	rice husk	279:287	untreated and treated rice husk (RH)	257:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	0	4	theme	starch	98:103	arg1	biocomposites					105:117	thermoplastic cassava starch biocomposites	76:117	thermoplastic cassava starch biocomposites	76:117	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	0	5	from	relationships	21:33	arg1	husk					60:63	alkaline treated rice husk	38:63	alkaline treated rice husk	38:63	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	6	6	theme	RH	1100:1101	arg1	biocomposites					1103:1115	the TPS/treated RH biocomposites	1084:1115	the TPS/treated RH biocomposites	1084:1115	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	6	7	theme	thermal	1063:1069	arg1	stability					1071:1079	the thermal stability	1059:1079	the thermal stability of the TPS/treated RH biocomposites	1059:1115	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	5	8	theme	%	944:944	arg1	RH					954:955	15 wt% treated RH	939:955	15 wt% treated RH	939:955	The biocomposite at 15 wt% treated RH showed high water absorption.					
33249147	5	9	theme	15 wt	939:943	arg1	RH					954:955	15 wt% treated RH	939:955	15 wt% treated RH	939:955	The biocomposite at 15 wt% treated RH showed high water absorption.					
33249147	2	10	theme	groups	514:519	arg1	population					496:505	a larger population	487:505	a larger population of -OH groups exposing on the fibril surface	487:550	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	3	11	theme	area	686:689	arg1	analysis					691:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	5	12	theme	treated	946:952	arg1	RH					954:955	15 wt% treated RH	939:955	15 wt% treated RH	939:955	The biocomposite at 15 wt% treated RH showed high water absorption.					
33249147	2	13	theme	RH	367:368	arg1	layer					358:362	the hemicellulose layer	340:362	the hemicellulose layer of RH	340:368	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	2	14	theme	-OH	510:512	arg1	groups					514:519	-OH groups	510:519	-OH groups	510:519	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	4	15	theme	improved	847:854	arg1	strength					864:871	substantially improved tensile strength	833:871	substantially improved tensile strength	833:871	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	2	16	theme	Alkaline	295:302	arg1	treatment					304:312	Alkaline treatment	295:312	Alkaline treatment with 11% w/v NaOH	295:330	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	2	17	theme	Fourier-transform	386:402	arg1	FTIR					427:430	FTIR	427:430	FTIR	427:430	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	2	17	theme	Fourier-transform	386:402	arg1	spectroscopy					413:424	Fourier-transform infrared spectroscopy	386:424	Fourier-transform infrared spectroscopy (FTIR)	386:431	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	1	18	theme	TPS	209:211	arg1	biocomposites					220:232	thermoplastic cassava starch (TPS) based biocomposites	179:232	thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	179:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	19	from	relationships	162:174	arg1	biocomposites					220:232	thermoplastic cassava starch (TPS) based biocomposites	179:232	thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	179:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	2	20	theme	infrared	404:411	arg1	FTIR					427:430	FTIR	427:430	FTIR	427:430	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	2	20	theme	infrared	404:411	arg1	spectroscopy					413:424	Fourier-transform infrared spectroscopy	386:424	Fourier-transform infrared spectroscopy (FTIR)	386:431	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	3	21	theme	surface	719:725	arg1	area					727:730	the surface area	715:730	the surface area of treated RH	715:744	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	6	22	theme	treated	1000:1006	arg1	contents					1011:1018	all treated RH contents	996:1018	all treated RH contents	996:1018	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	1	23	theme	based	214:218	arg1	biocomposites					220:232	thermoplastic cassava starch (TPS) based biocomposites	179:232	thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	179:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	0	24	theme	Structure-properties	0:19	arg1	relationships					21:33	Structure-properties relationships	0:33	Structure-properties relationships in alkaline treated rice husk	0:63	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	4	25	theme	tensile	856:862	arg1	strength					864:871	substantially improved tensile strength	833:871	substantially improved tensile strength	833:871	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	2	26	theme	w/v	323:325	arg1	NaOH					327:330	11% w/v NaOH	319:330	11% w/v NaOH	319:330	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	6	27	theme	biodegradation	1032:1045	arg1	rate					1047:1050	high biodegradation rate	1027:1050	high biodegradation rate	1027:1050	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	0	28	theme	alkaline	38:45	arg1	husk					60:63	alkaline treated rice husk	38:63	alkaline treated rice husk	38:63	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	4	29	theme	neat	909:912	arg1	TPS					914:916	the neat TPS	905:916	the neat TPS	905:916	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	2	30	theme	%	321:321	arg1	NaOH					327:330	11% w/v NaOH	319:330	11% w/v NaOH	319:330	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	6	31	theme	high	1027:1030	arg1	rate					1047:1050	high biodegradation rate	1027:1050	high biodegradation rate	1027:1050	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	2	32	theme	thermogravimetric	437:453	arg1	TGA					465:467	TGA	465:467	TGA	465:467	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	2	32	theme	thermogravimetric	437:453	arg1	analysis					455:462	thermogravimetric analysis	437:462	thermogravimetric analysis (TGA)	437:468	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	3	33	theme	treated	735:741	arg1	RH					743:744	treated RH	735:744	treated RH	735:744	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	2	34	theme	fibril	537:542	arg1	surface					544:550	the fibril surface	533:550	the fibril surface	533:550	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	2	35	theme	11	319:320	arg1	%					321:321	%	321:321	%	321:321	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	1	36	theme	5-20 wt	245:251	arg1	RH					290:291	RH	290:291	RH	290:291	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	36	theme	5-20 wt	245:251	arg1	%					252:252	5-20 wt%	245:252	5-20 wt% of untreated and treated rice husk (RH)	245:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	36	theme	5-20 wt	245:251	arg1	husk					284:287	rice husk	279:287	untreated and treated rice husk (RH)	257:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	0	37	theme	rice	55:58	arg1	husk					60:63	alkaline treated rice husk	38:63	alkaline treated rice husk	38:63	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	2	38	theme	hemicellulose	344:356	arg1	layer					358:362	the hemicellulose layer	340:362	the hemicellulose layer of RH	340:368	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	3	39	theme	Brunauer-Emmett-Teller	649:670	arg1	analysis					691:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	4	40	theme	treated	815:821	arg1	RH					823:824	20 wt% treated RH	808:824	20 wt% treated RH	808:824	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	4	41	dep	treated	815:821	arg1	%					813:813	20 wt%	808:813	20 wt%	808:813	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	0	42	theme	treated	47:53	arg1	husk					60:63	alkaline treated rice husk	38:63	alkaline treated rice husk	38:63	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	3	43	theme	treated	606:612	arg1	RH					614:615	treated RH	606:615	treated RH	606:615	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	6	44	theme	RH	1008:1009	arg1	contents					1011:1018	all treated RH contents	996:1018	all treated RH contents	996:1018	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	4	45	theme	%	891:891	arg1	factor					878:883	a factor	876:883	a factor of 220%	876:891	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	2	46	theme	larger	489:494	arg1	population					496:505	a larger population	487:505	a larger population of -OH groups exposing on the fibril surface	487:550	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	3	47	theme	filler-matrix	571:583	arg1	interactions					585:596	the filler-matrix interactions	567:596	the filler-matrix interactions between treated RH and TPS	567:623	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	6	48	theme	biocomposites	1103:1115	arg1	stability					1071:1079	the thermal stability	1059:1079	the thermal stability of the TPS/treated RH biocomposites	1059:1115	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	1	49	theme	structure-properties	141:160	arg1	relationships					162:174	structure-properties relationships	141:174	structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	141:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	3	50	theme	surface	678:684	arg1	analysis					691:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	5	51	theme	high	964:967	arg1	absorption					975:984	high water absorption	964:984	high water absorption	964:984	The biocomposite at 15 wt% treated RH showed high water absorption.					
33249147	6	52	with	TPS	987:989	arg1	contents					1011:1018	all treated RH contents	996:1018	all treated RH contents	996:1018	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	6	53	theme	TPS/treated	1088:1098	arg1	biocomposites					1103:1115	the TPS/treated RH biocomposites	1084:1115	the TPS/treated RH biocomposites	1084:1115	TPS with all treated RH contents showed high biodegradation rate, while the thermal stability of the TPS/treated RH biocomposites was slightly decreased.					
33249147	1	54	theme	untreated	257:265	arg1	RH					290:291	RH	290:291	RH	290:291	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	54	theme	untreated	257:265	arg1	husk					284:287	rice husk	279:287	untreated and treated rice husk (RH)	257:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	5	55	theme	water	969:973	arg1	absorption					975:984	high water absorption	964:984	high water absorption	964:984	The biocomposite at 15 wt% treated RH showed high water absorption.					
33249147	0	56	theme	cassava	90:96	arg1	biocomposites					105:117	thermoplastic cassava starch biocomposites	76:117	thermoplastic cassava starch biocomposites	76:117	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	3	57	theme	BET	673:675	arg1	analysis					691:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Brunauer-Emmett-Teller (BET) surface area analysis	649:698	Consequently, the filler-matrix interactions between treated RH and TPS were enhanced, although Brunauer-Emmett-Teller (BET) surface area analysis indicated that the surface area of treated RH was not increased.					
33249147	4	58	theme	20 wt	808:812	arg1	%					813:813	20 wt%	808:813	20 wt%	808:813	Interestingly, the biocomposites contained 20 wt% treated RH showed substantially improved tensile strength by a factor of 220% compared to the neat TPS.					
33249147	1	59	theme	thermoplastic	179:191	arg1	biocomposites					220:232	thermoplastic cassava starch (TPS) based biocomposites	179:232	thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	179:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	60	theme	treated	271:277	arg1	RH					290:291	RH	290:291	RH	290:291	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	60	theme	treated	271:277	arg1	husk					284:287	rice husk	279:287	untreated and treated rice husk (RH)	257:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	0	61	theme	thermoplastic	76:88	arg1	biocomposites					105:117	thermoplastic cassava starch biocomposites	76:117	thermoplastic cassava starch biocomposites	76:117	Structure-properties relationships in alkaline treated rice husk reinforced thermoplastic cassava starch biocomposites.					
33249147	7	62	theme	promising	1171:1179	arg1	properties					1181:1190	promising properties	1171:1190	promising properties for applications as absorbents	1171:1221	These novel composites showed promising properties for applications as absorbents.					
33249147	2	63	with	treatment	304:312	arg1	NaOH					327:330	11% w/v NaOH	319:330	11% w/v NaOH	319:330	Alkaline treatment with 11% w/v NaOH removed the hemicellulose layer of RH as confirmed by Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and resulted in a larger population of -OH groups exposing on the fibril surface.					
33249147	1	64	theme	cassava	193:199	arg1	biocomposites					220:232	thermoplastic cassava starch (TPS) based biocomposites	179:232	thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH)	179:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	5	65	from	RH	954:955	arg1	biocomposite					923:934	The biocomposite	919:934	The biocomposite at 15 wt% treated RH	919:955	The biocomposite at 15 wt% treated RH showed high water absorption.					
33249147	1	66	theme	rice	279:282	arg1	RH					290:291	RH	290:291	RH	290:291	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
33249147	1	66	theme	rice	279:282	arg1	husk					284:287	rice husk	279:287	untreated and treated rice husk (RH)	257:292	The study focuses on structure-properties relationships in thermoplastic cassava starch (TPS) based biocomposites comprising 5-20 wt% of untreated and treated rice husk (RH).					
34400694	5	0	theme	quince	748:753	arg1	mucilage					755:762	quince mucilage	748:762	quince mucilage	748:762	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	3	1	theme	compatible	469:478	arg1	blends					480:485	natural and compatible blends	457:485	natural and compatible blends of different polysaccharides that have demonstrated medical benefits	457:554	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	3	1	theme	compatible	469:478	arg1	mucilages					443:451	These mucilages	437:451	These mucilages	437:451	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	4	2	theme	seed	567:570	arg1	films					581:585	All three seed mucilage films	557:585	All three seed mucilage films	557:585	All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food.					
34400694	8	3	theme	%	1318:1318	arg1	coatings					1360:1367	100% natural mucilage-based probiotic edible coatings	1315:1367	100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1315:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	7	4	theme	quince-based	1129:1140	arg1	films					1152:1156	quince-based probiotic films	1129:1156	quince-based probiotic films	1129:1156	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	1	5	theme	public	157:162	arg1	community					179:187	the public and scientific community	153:187	community	179:187	There is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome.					
34400694	8	6	theme	first	1247:1251	arg1	report					1253:1258	the first report	1243:1258	the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1243:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	6	theme	first	1247:1251	arg1	This					1235:1238	This	1235:1238	This	1235:1238	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	6	7	theme	probiotics	980:989	arg1	viability					991:999	the probiotics viability	976:999	the probiotics viability	976:999	These films effectively protected fruits against UV light, maintaining the probiotics viability and inactivation rate during storage.					
34400694	0	8	theme	fruits	98:103	arg1	preservation					82:93	the preservation	78:93	the preservation of fruits and vegetables	78:118	Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables.					
34400694	8	9	theme	coatings	1360:1367	arg1	development					1267:1277	development	1267:1277	development	1267:1277	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	9	theme	coatings	1360:1367	arg1	production					1301:1310	production	1301:1310	production	1301:1310	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	9	theme	coatings	1360:1367	arg1	characterization					1280:1295	characterization	1280:1295	characterization	1280:1295	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	7	10	theme	morphological	1196:1208	arg1	viability					1224:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	8	11	theme	edible	1353:1358	arg1	coatings					1360:1367	100% natural mucilage-based probiotic edible coatings	1315:1367	100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1315:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	1	12	theme	growing	130:136	arg1	interest					138:145	growing interest	130:145	growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome	130:277	There is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome.					
34400694	0	13	theme	vegetables	109:118	arg1	preservation					82:93	the preservation	78:93	the preservation of fruits and vegetables	78:118	Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables.					
34400694	5	14	theme	mucilage	889:896	arg1	films					898:902	basil mucilage films	883:902	basil mucilage films	883:902	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	5	15	from	break	872:876	arg1	at					869:870	elongation at break	858:876	elongation at break	858:876	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	5	15	from	break	872:876	arg1	elongation					858:867	elongation at break	858:876	elongation at break	858:876	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	3	16	theme	polysaccharides	500:514	arg1	blends					480:485	natural and compatible blends	457:485	natural and compatible blends of different polysaccharides that have demonstrated medical benefits	457:554	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	3	16	theme	polysaccharides	500:514	arg1	mucilages					443:451	These mucilages	437:451	These mucilages	437:451	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	4	17	theme	probiotics	651:660	arg1	presence					639:646	the presence	635:646	the presence of probiotics, which is needed to help preserve the moisture/freshness of food	635:725	All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food.					
34400694	1	18	theme	gut	264:266	arg1	microbiome					268:277	the gut microbiome	260:277	the gut microbiome	260:277	There is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome.					
34400694	8	19	contain	containing	1437:1446	arg2	probiotics					1448:1457	probiotics	1448:1457	probiotics	1448:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	19	contain	containing	1437:1446	arg1	applications					1424:1435	food preservation applications	1406:1435	food preservation applications containing probiotics	1406:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	7	20	theme	probiotic	1142:1150	arg1	films					1152:1156	quince-based probiotic films	1129:1156	quince-based probiotic films	1129:1156	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	7	21	theme	physical	1186:1193	arg1	viability					1224:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	1	22	theme	scientific	168:177	arg1	community					179:187	the public and scientific community	153:187	community	179:187	There is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome.					
34400694	2	23	theme	eco-friendly	306:317	arg1	films					336:340	preparing eco-friendly probiotic edible films	296:340	preparing eco-friendly probiotic edible films	296:340	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	2	24	theme	probiotics	371:380	arg1	addition					359:366	the addition	355:366	the addition of probiotics to the seed mucilage films of quince, flax, and basil	355:434	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	2	25	theme	flax	420:423	arg1	films					403:407	the seed mucilage films	385:407	the seed mucilage films of quince, flax, and basil	385:434	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	1	26	theme	microbiome	268:277	arg1	composition					245:255	the composition	241:255	the composition of the gut microbiome	241:277	There is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome.					
34400694	7	27	theme	longer	1093:1098	arg1	freshness					1083:1091	their freshness	1077:1091	their freshness longer than uncoated produce	1077:1120	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	5	28	theme	elastic	839:845	arg1	moduli					847:852	higher elastic moduli	832:852	higher elastic moduli	832:852	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	2	29	theme	preparing	296:304	arg1	films					336:340	preparing eco-friendly probiotic edible films	296:340	preparing eco-friendly probiotic edible films	296:340	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	8	30	with	coatings	1360:1367	arg1	properties					1391:1400	enhanced barrier properties	1374:1400	enhanced barrier properties for food preservation applications containing probiotics	1374:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	5	31	theme	elongation	858:867	arg1	break					872:876	elongation at break	858:876	elongation at break	858:876	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	4	32	theme	food	722:725	arg1	moisture/freshness					700:717	the moisture/freshness	696:717	the moisture/freshness of food	696:725	All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food.					
34400694	8	33	theme	production	1301:1310	arg1	report					1253:1258	the first report	1243:1258	the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1243:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	33	theme	production	1301:1310	arg1	This					1235:1238	This	1235:1238	This	1235:1238	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	3	34	theme	medical	539:545	arg1	benefits					547:554	medical benefits	539:554	medical benefits	539:554	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	8	35	theme	development	1267:1277	arg1	report					1253:1258	the first report	1243:1258	the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1243:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	35	theme	development	1267:1277	arg1	This					1235:1238	This	1235:1238	This	1235:1238	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	2	36	theme	quince	412:417	arg1	films					403:407	the seed mucilage films	385:407	the seed mucilage films of quince, flax, and basil	385:434	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	6	37	theme	UV	954:955	arg1	light					957:961	UV light	954:961	UV light	954:961	These films effectively protected fruits against UV light, maintaining the probiotics viability and inactivation rate during storage.					
34400694	8	38	theme	enhanced	1374:1381	arg1	properties					1391:1400	enhanced barrier properties	1374:1400	enhanced barrier properties for food preservation applications containing probiotics	1374:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	5	39	theme	basil	883:887	arg1	films					898:902	basil mucilage films	883:902	basil mucilage films	883:902	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	8	40	theme	natural	1320:1326	arg1	coatings					1360:1367	100% natural mucilage-based probiotic edible coatings	1315:1367	100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1315:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	0	41	theme	mucilage	46:53	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables.					
34400694	0	41	theme	mucilage	46:53	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables.					
34400694	8	42	theme	mucilage-based	1328:1341	arg1	coatings					1360:1367	100% natural mucilage-based probiotic edible coatings	1315:1367	100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1315:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	43	theme	characterization	1280:1295	arg1	report					1253:1258	the first report	1243:1258	the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1243:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	43	theme	characterization	1280:1295	arg1	This					1235:1238	This	1235:1238	This	1235:1238	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	2	44	theme	mucilage	394:401	arg1	films					403:407	the seed mucilage films	385:407	the seed mucilage films of quince, flax, and basil	385:434	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	0	45	theme	probiotic	36:44	arg1	mucilage					46:53	probiotic mucilage	36:53	probiotic mucilage	36:53	Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables.					
34400694	5	46	with	robust	820:825	arg1	moduli					847:852	higher elastic moduli	832:852	higher elastic moduli	832:852	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	5	46	with	robust	820:825	arg1	break					872:876	elongation at break	858:876	elongation at break	858:876	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	6	47	theme	inactivation	1005:1016	arg1	rate					1018:1021	inactivation rate	1005:1021	inactivation rate	1005:1021	These films effectively protected fruits against UV light, maintaining the probiotics viability and inactivation rate during storage.					
34400694	5	48	theme	higher	832:837	arg1	moduli					847:852	higher elastic moduli	832:852	higher elastic moduli	832:852	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	1	49	theme	probiotics	207:216	arg1	use					200:202	the use	196:202	the use of probiotics to potentially restore the composition of the gut microbiome	196:277	There is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome.					
34400694	0	50	theme	edible	61:66	arg1	films					68:72	edible films	61:72	edible films for the preservation of fruits and vegetables	61:118	Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables.					
34400694	7	51	theme	Coated	1039:1044	arg1	fruits					1046:1051	Coated fruits	1039:1051	Coated fruits	1039:1051	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	8	52	theme	barrier	1383:1389	arg1	properties					1391:1400	enhanced barrier properties	1374:1400	enhanced barrier properties for food preservation applications containing probiotics	1374:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	3	53	theme	natural	457:463	arg1	blends					480:485	natural and compatible blends	457:485	natural and compatible blends of different polysaccharides that have demonstrated medical benefits	457:554	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	3	53	theme	natural	457:463	arg1	mucilages					443:451	These mucilages	437:451	These mucilages	437:451	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	7	54	dep	uncoated	1105:1112	arg1	produce					1114:1120	produce	1114:1120	produce	1114:1120	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	7	55	theme	bacterial	1214:1222	arg1	viability					1224:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	2	56	theme	films	336:340	arg1	aim					289:291	the aim	285:291	the aim of preparing eco-friendly probiotic edible films	285:340	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	5	57	from	mucilage	755:762	arg1	Films					728:732	Films	728:732	Films from flax and quince mucilage	728:762	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	8	58	dep	development	1267:1277	arg1	the					1263:1265	the	1263:1265	the	1263:1265	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	59	theme	preservation	1411:1422	arg1	applications					1424:1435	food preservation applications	1406:1435	food preservation applications containing probiotics	1406:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	2	60	theme	edible	329:334	arg1	films					336:340	preparing eco-friendly probiotic edible films	296:340	preparing eco-friendly probiotic edible films	296:340	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	8	61	theme	food	1406:1409	arg1	applications					1424:1435	food preservation applications	1406:1435	food preservation applications containing probiotics	1406:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	4	62	theme	high	597:600	arg1	retention					611:619	high moisture retention	597:619	high moisture retention	597:619	All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food.					
34400694	5	63	from	flax	739:742	arg1	Films					728:732	Films	728:732	Films from flax and quince mucilage	728:762	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	2	64	theme	probiotic	319:327	arg1	films					336:340	preparing eco-friendly probiotic edible films	296:340	preparing eco-friendly probiotic edible films	296:340	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	8	65	theme	probiotic	1343:1351	arg1	coatings					1360:1367	100% natural mucilage-based probiotic edible coatings	1315:1367	100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics	1315:1457	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	8	66	theme	100	1315:1317	arg1	%					1318:1318	%	1318:1318	%	1318:1318	This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.					
34400694	2	67	theme	seed	389:392	arg1	films					403:407	the seed mucilage films	385:407	the seed mucilage films of quince, flax, and basil	385:434	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	5	68	theme	at	869:870	arg1	break					872:876	elongation at break	858:876	elongation at break	858:876	Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films.					
34400694	4	69	theme	mucilage	572:579	arg1	films					581:585	All three seed mucilage films	557:585	All three seed mucilage films	557:585	All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food.					
34400694	2	70	theme	basil	430:434	arg1	films					403:407	the seed mucilage films	385:407	the seed mucilage films of quince, flax, and basil	385:434	With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil.					
34400694	7	71	theme	mechanical	1174:1183	arg1	viability					1224:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	the best mechanical, physical, morphological and bacterial viability	1165:1232	Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability.					
34400694	3	72	theme	different	490:498	arg1	polysaccharides					500:514	different polysaccharides	490:514	different polysaccharides	490:514	These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits.					
34400694	4	73	theme	moisture	602:609	arg1	retention					611:619	high moisture retention	597:619	high moisture retention	597:619	All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food.					
34679232	6	0	theme	line	951:954	arg1	use					913:915	The use	909:915	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line	909:987	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	2	1	theme	high	346:349	arg1	concentrations					356:369	high cell concentrations	346:369	high cell concentrations	346:369	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	1	2	theme	engineering	242:252	arg1	field					226:230	the field	222:230	the field of tissue engineering	222:252	Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering.					
34679232	6	3	theme	alginate	1101:1108	arg1	microspheres					1110:1121	the alginate microspheres	1097:1121	the alginate microspheres	1097:1121	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	6	4	theme	cell	979:982	arg1	line					984:987	an example cell line	968:987	an example cell line	968:987	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	0	5	theme	Defined	108:114	arg1	Pockets					121:127	Spatially Defined Cell Pockets	98:127	Spatially Defined Cell Pockets	98:127	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	2	6	theme	fabrication	277:287	arg1	method					289:294	a facile 2-step fabrication method	261:294	a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment	261:414	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	6	7	theme	example	971:977	arg1	line					984:987	an example cell line	968:987	an example cell line	968:987	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	2	8	theme	2-step	270:275	arg1	method					289:294	a facile 2-step fabrication method	261:294	a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment	261:414	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	0	9	theme	Pockets	121:127	arg1	Fabrication					83:93	the Fabrication	79:93	the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels	79:152	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	3	10	dep	other	520:524	arg1	relevant					532:539	relevant	532:539	relevant	532:539	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	2	11	theme	facile	263:268	arg1	method					289:294	a facile 2-step fabrication method	261:294	a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment	261:414	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	4	12	theme	layered	648:654	arg1	structure					656:664	A layered structure	646:664	A layered structure at the macroscale	646:682	A layered structure at the macroscale can be achieved by incorporating these cell-containing microspheres in thin collagen layers.					
34679232	0	13	theme	Cell	116:119	arg1	Pockets					121:127	Spatially Defined Cell Pockets	98:127	Spatially Defined Cell Pockets	98:127	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	4	14	theme	collagen	760:767	arg1	layers					769:774	thin collagen layers	755:774	thin collagen layers	755:774	A layered structure at the macroscale can be achieved by incorporating these cell-containing microspheres in thin collagen layers.					
34679232	1	15	theme	natural	183:189	arg1	tissue					191:196	natural tissue	183:196	natural tissue	183:196	Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering.					
34679232	6	16	theme	cell	946:949	arg1	line					951:954	the murine pre-osteoblast cell line	920:954	the murine pre-osteoblast cell line	920:954	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	2	17	theme	3D	307:308	arg1	constructs					310:319	3D constructs	307:319	3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment	307:414	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	1	18	theme	tissue	191:196	arg1	complexity					169:178	the complexity	165:178	the complexity of natural tissue	165:196	Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering.					
34679232	4	19	theme	thin	755:758	arg1	layers					769:774	thin collagen layers	755:774	thin collagen layers	755:774	A layered structure at the macroscale can be achieved by incorporating these cell-containing microspheres in thin collagen layers.					
34679232	2	20	with	constructs	310:319	arg1	regions					335:341	distinct regions	326:341	distinct regions of high cell concentrations	326:369	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	0	21	theme	3D	132:133	arg1	Hydrogels					144:152	3D Collagen Hydrogels	132:152	3D Collagen Hydrogels	132:152	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	3	22	theme	biopolymers	541:551	arg1	addition					508:515	the addition	504:515	the addition of other, cell relevant biopolymers	504:551	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	3	23	theme	cells	458:462	arg1	incorporation					441:453	The initial incorporation	429:453	The initial incorporation of cells in a sacrificial alginate matrix	429:495	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	0	24	theme	Temporary	15:23	arg1	Microcarriers					51:63	Temporary, Cell-Containing Alginate Microcarriers	15:63	Temporary, Cell-Containing Alginate Microcarriers	15:63	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	3	25	from	incorporation	441:453	arg1	matrix					490:495	a sacrificial alginate matrix	467:495	a sacrificial alginate matrix	467:495	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	3	26	theme	other	520:524	arg1	biopolymers					541:551	other, cell relevant biopolymers	520:551	other, cell relevant biopolymers	520:551	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	4	27	theme	cell-containing	723:737	arg1	microspheres					739:750	these cell-containing microspheres	717:750	these cell-containing microspheres	717:750	A layered structure at the macroscale can be achieved by incorporating these cell-containing microspheres in thin collagen layers.					
34679232	1	28	theme	major	203:207	arg1	challenge					209:217	a major challenge	201:217	a major challenge in the field of tissue engineering	201:252	Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering.					
34679232	0	29	theme	Alginate	42:49	arg1	Microcarriers					51:63	Temporary, Cell-Containing Alginate Microcarriers	15:63	Temporary, Cell-Containing Alginate Microcarriers	15:63	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	2	30	dep	method	289:294	arg1	prepare					299:305	prepare	299:305	to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment	296:414	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	0	31	from	Fabrication	83:93	arg1	Hydrogels					144:152	3D Collagen Hydrogels	132:152	3D Collagen Hydrogels	132:152	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	3	32	dep	form	575:578	arg1	such					554:557	such	554:557	such	554:557	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	3	33	dep	confined	592:599	arg1	interpenetrating					602:617	interpenetrating	602:617	interpenetrating	602:617	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	6	34	theme	pre-osteoblast	931:944	arg1	line					951:954	the murine pre-osteoblast cell line	920:954	the murine pre-osteoblast cell line	920:954	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	0	35	theme	Collagen	135:142	arg1	Hydrogels					144:152	3D Collagen Hydrogels	132:152	3D Collagen Hydrogels	132:152	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	1	36	from	challenge	209:217	arg1	field					226:230	the field	222:230	the field of tissue engineering	222:252	Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering.					
34679232	6	37	theme	migration	1043:1051	arg1	pattern					1053:1059	their cell migration pattern	1032:1059	their cell migration pattern based on the initial composition of the alginate microspheres	1032:1121	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	5	38	theme	hydrogel	875:882	arg1	layers					884:889	the collagen hydrogel layers	862:889	the collagen hydrogel layers	862:889	Cells are locally released by de-gelling the alginate matrix and their attachment to the collagen hydrogel layers has been studied.					
34679232	3	39	theme	alginate	481:488	arg1	matrix					490:495	a sacrificial alginate matrix	467:495	a sacrificial alginate matrix	467:495	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	0	40	theme	Microcarriers	51:63	arg1	Application					0:10	Application	0:10	Application of Temporary, Cell-Containing Alginate Microcarriers	0:63	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	6	41	theme	cell	1038:1041	arg1	pattern					1053:1059	their cell migration pattern	1032:1059	their cell migration pattern based on the initial composition of the alginate microspheres	1032:1121	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	2	42	theme	distinct	326:333	arg1	regions					335:341	distinct regions	326:341	distinct regions of high cell concentrations	326:369	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	3	43	theme	confined	592:599	arg1	network					619:625	a spatially confined, interpenetrating network	580:625	a spatially confined, interpenetrating network	580:625	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	5	44	theme	collagen	866:873	arg1	layers					884:889	the collagen hydrogel layers	862:889	the collagen hydrogel layers	862:889	Cells are locally released by de-gelling the alginate matrix and their attachment to the collagen hydrogel layers has been studied.					
34679232	6	45	theme	murine	924:929	arg1	line					951:954	the murine pre-osteoblast cell line	920:954	the murine pre-osteoblast cell line	920:954	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	0	46	dep	Temporary	15:23	arg1	Cell-Containing					26:40	Cell-Containing	26:40	Cell-Containing	26:40	Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels.					
34679232	3	47	theme	sacrificial	469:479	arg1	matrix					490:495	a sacrificial alginate matrix	467:495	a sacrificial alginate matrix	467:495	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	6	48	theme	microspheres	1110:1121	arg1	composition					1082:1092	the initial composition	1070:1092	the initial composition of the alginate microspheres	1070:1121	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	6	49	theme	initial	1074:1080	arg1	composition					1082:1092	the initial composition	1070:1092	the initial composition of the alginate microspheres	1070:1121	The use of the murine pre-osteoblast cell line MC3T3-E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.					
34679232	5	50	theme	alginate	822:829	arg1	matrix					831:836	the alginate matrix	818:836	the alginate matrix	818:836	Cells are locally released by de-gelling the alginate matrix and their attachment to the collagen hydrogel layers has been studied.					
34679232	2	51	theme	concentrations	356:369	arg1	regions					335:341	distinct regions	326:341	distinct regions of high cell concentrations	326:369	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	3	52	theme	initial	433:439	arg1	incorporation					441:453	The initial incorporation	429:453	The initial incorporation of cells in a sacrificial alginate matrix	429:495	The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale.					
34679232	2	53	theme	cell	351:354	arg1	concentrations					356:369	high cell concentrations	346:369	high cell concentrations	346:369	Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed.					
34679232	4	54	from	macroscale	673:682	arg1	structure					656:664	A layered structure	646:664	A layered structure at the macroscale	646:682	A layered structure at the macroscale can be achieved by incorporating these cell-containing microspheres in thin collagen layers.					
34679232	1	55	theme	tissue	235:240	arg1	engineering					242:252	tissue engineering	235:252	tissue engineering	235:252	Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering.					
34617945	1	0	theme	nucifera	140:147	arg1	NNLP					170:173	NNLP	170:173	NNLP	170:173	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	1	0	theme	nucifera	140:147	arg1	polysaccharide					154:167	the Nelumbo nucifera leaf polysaccharide	128:167	the Nelumbo nucifera leaf polysaccharide (NNLP)	128:174	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	1	1	theme	leaf	149:152	arg1	NNLP					170:173	NNLP	170:173	NNLP	170:173	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	1	1	theme	leaf	149:152	arg1	polysaccharide					154:167	the Nelumbo nucifera leaf polysaccharide	128:167	the Nelumbo nucifera leaf polysaccharide (NNLP)	128:174	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	5	2	from	stress	999:1004	arg1	cells					978:982	cultured intestinal cells	958:982	cultured intestinal cells from oxidative stress	958:1004	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	4	3	dep	backbone	640:647	arg1	a					621:621	a	621:621	a	621:621	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	2	4	theme	gel	279:281	arg1	filtration					283:292	gel filtration	279:292	gel filtration	279:292	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	5	5	theme	intestinal	967:976	arg1	cells					978:982	cultured intestinal cells	958:982	cultured intestinal cells from oxidative stress	958:1004	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	4	6	theme	linked	745:750	arg1	rhamnose					752:759	1-3 linked rhamnose	741:759	1-3 linked rhamnose as side chains	741:774	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	2	7	theme	NNLP-I-I	351:358	arg1	fraction					342:349	the purified fraction	329:349	the purified fraction NNLP-I-I	329:358	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	4	8	link	linked	745:750	arg1	rhamnose					752:759	1-3 linked rhamnose	741:759	1-3 linked rhamnose as side chains	741:774	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	4	9	theme	RG-I	713:716	arg1	region					718:723	short RG-I region	707:723	short RG-I region	707:723	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	5	10	theme	antioxidant	837:847	arg1	properties					849:858	antioxidant properties	837:858	antioxidant properties	837:858	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	4	11	theme	side	764:767	arg1	chains					769:774	side chains	764:774	side chains	764:774	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	2	12	theme	which	385:389	arg1	kDa					400:402	16.4 kDa	395:402	16.4 kDa	395:402	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	2	12	theme	which	385:389	arg1	weight					375:380	the molecular weight	361:380	the molecular weight	361:380	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	2	13	theme	exchange	251:258	arg1	chromatography					260:273	DEAE anion exchange chromatography	240:273	DEAE anion exchange chromatography	240:273	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	0	14	from	nucifera	35:42	arg1	polysaccharide					7:20	Pectic polysaccharide	0:20	Pectic polysaccharide from Nelumbo nucifera	0:42	Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo.					
34617945	2	15	dep	obtained	320:327	arg1	kDa					400:402	16.4 kDa	395:402	16.4 kDa	395:402	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	2	15	dep	obtained	320:327	arg1	weight					375:380	the molecular weight	361:380	the molecular weight	361:380	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	5	16	theme	Nrf2-regulated	882:895	arg1	defense					929:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	0	17	theme	Pectic	0:5	arg1	polysaccharide					7:20	Pectic polysaccharide	0:20	Pectic polysaccharide from Nelumbo nucifera	0:42	Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo.					
34617945	5	18	theme	antioxidant	917:927	arg1	defense					929:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	2	19	theme	molecular	365:373	arg1	kDa					400:402	16.4 kDa	395:402	16.4 kDa	395:402	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	2	19	theme	molecular	365:373	arg1	weight					375:380	the molecular weight	361:380	the molecular weight	361:380	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	5	20	theme	cultured	958:965	arg1	cells					978:982	cultured intestinal cells	958:982	cultured intestinal cells from oxidative stress	958:1004	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	3	21	theme	units	457:461	arg1	determination					463:475	linkage units determination	449:475	linkage units determination	449:475	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	4	22	theme	spectra	561:567	arg1	analysis					569:576	the NMR spectra analysis	553:576	the NMR spectra analysis	553:576	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	5	23	theme	oxidative	989:997	arg1	stress					999:1004	oxidative stress	989:1004	oxidative stress	989:1004	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	4	24	theme	homogalacturonan	623:638	arg1	backbone					640:647	homogalacturonan backbone	623:647	homogalacturonan backbone	623:647	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	4	25	theme	NMR	557:559	arg1	analysis					569:576	the NMR spectra analysis	553:576	the NMR spectra analysis	553:576	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	5	26	theme	mice	1050:1053	arg1	function					1033:1040	the intestinal function	1018:1040	the intestinal function of aged mice	1018:1053	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	3	27	theme	monosaccharide	409:422	arg1	composition					424:434	The monosaccharide composition	405:434	The monosaccharide composition analysis	405:443	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	4	28	theme	rhamnogalacturonan	653:670	arg1	I					672:672	rhamnogalacturonan I	653:672	rhamnogalacturonan I	653:672	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	3	29	theme	composition	424:434	arg1	analysis					436:443	The monosaccharide composition analysis	405:443	The monosaccharide composition analysis	405:443	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	4	30	theme	short	707:711	arg1	region					718:723	short RG-I region	707:723	short RG-I region	707:723	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	1	31	theme	hot	192:194	arg1	extraction					202:211	hot water extraction	192:211	hot water extraction	192:211	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	0	32	theme	intestinal	60:69	arg1	defense					83:89	intestinal antioxidant defense	60:89	intestinal antioxidant defense	60:89	Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo.					
34617945	5	33	theme	cellular	908:915	arg1	defense					929:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	4	34	with	I	672:672	arg1	rhamnose					752:759	1-3 linked rhamnose	741:759	1-3 linked rhamnose as side chains	741:774	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	4	34	with	I	672:672	arg1	AG-II					731:735	AG-II	731:735	AG-II	731:735	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	0	35	dep	leaves	44:49	arg1	promotes					51:58	promotes	51:58	leaves promotes intestinal antioxidant defense in vitro and in vivo	44:110	Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo.					
34617945	2	36	theme	purified	333:340	arg1	fraction					342:349	the purified fraction	329:349	the purified fraction NNLP-I-I	329:358	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	5	37	theme	biological	781:790	arg1	studies					792:798	The biological studies	777:798	The biological studies	777:798	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	4	38	contain	containing	675:684	arg2	region					718:723	short RG-I region	707:723	short RG-I region	707:723	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	4	38	contain	containing	675:684	arg1	backbone					640:647	homogalacturonan backbone	623:647	homogalacturonan backbone	623:647	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	4	38	contain	containing	675:684	arg2	region					696:701	a long HG region	686:701	a long HG region	686:701	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	3	39	theme	fraction	493:500	arg1	polysaccharide					524:537	a pectic polysaccharide	515:537	a pectic polysaccharide	515:537	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	3	39	theme	fraction	493:500	arg1	NNLP-I-I					502:509	the fraction NNLP-I-I	489:509	the fraction NNLP-I-I	489:509	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	5	40	theme	intestinal	1022:1031	arg1	function					1033:1040	the intestinal function	1018:1040	the intestinal function of aged mice	1018:1053	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	2	41	theme	anion	245:249	arg1	chromatography					260:273	DEAE anion exchange chromatography	240:273	DEAE anion exchange chromatography	240:273	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	4	42	theme	long	688:691	arg1	region					696:701	a long HG region	686:701	a long HG region	686:701	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	5	43	theme	intestinal	897:906	arg1	defense					929:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	the Nrf2-regulated intestinal cellular antioxidant defense	878:935	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	1	44	theme	water	196:200	arg1	extraction					202:211	hot water extraction	192:211	hot water extraction	192:211	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	4	45	with	backbone	640:647	arg1	rhamnose					752:759	1-3 linked rhamnose	741:759	1-3 linked rhamnose as side chains	741:774	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	4	45	with	backbone	640:647	arg1	AG-II					731:735	AG-II	731:735	AG-II	731:735	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	2	46	theme	DEAE	240:243	arg1	chromatography					260:273	DEAE anion exchange chromatography	240:273	DEAE anion exchange chromatography	240:273	DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa.					
34617945	3	47	theme	pectic	517:522	arg1	polysaccharide					524:537	a pectic polysaccharide	515:537	a pectic polysaccharide	515:537	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	3	47	theme	pectic	517:522	arg1	NNLP-I-I					502:509	the fraction NNLP-I-I	489:509	the fraction NNLP-I-I	489:509	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	3	48	theme	linkage	449:455	arg1	units					457:461	linkage units	449:461	linkage units determination	449:475	The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide.					
34617945	4	49	theme	HG	693:694	arg1	region					696:701	a long HG region	686:701	a long HG region	686:701	In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains.					
34617945	0	50	theme	antioxidant	71:81	arg1	defense					83:89	intestinal antioxidant defense	60:89	intestinal antioxidant defense	60:89	Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo.					
34617945	5	51	theme	aged	1045:1048	arg1	mice					1050:1053	aged mice	1045:1053	aged mice	1045:1053	The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.					
34617945	1	52	theme	Nelumbo	132:138	arg1	NNLP					170:173	NNLP	170:173	NNLP	170:173	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	1	52	theme	Nelumbo	132:138	arg1	polysaccharide					154:167	the Nelumbo nucifera leaf polysaccharide	128:167	the Nelumbo nucifera leaf polysaccharide (NNLP)	128:174	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
34617945	1	53	theme	ethanol	217:223	arg1	precipitation					225:237	ethanol precipitation	217:237	ethanol precipitation	217:237	In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation.					
32910962	0	0	theme	bone	73:76	arg1	engineering					85:95	bone tissue engineering	73:95	bone tissue engineering	73:95	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
32910962	5	1	theme	Energy	683:688	arg1	analysis					713:720	Energy dispersive x-ray (EDS) analysis	683:720	Energy dispersive x-ray (EDS) analysis	683:720	The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis.					
32910962	2	2	theme	mesoporous	323:332	arg1	mZS					349:351	mZS	349:351	mZS	349:351	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	2	theme	mesoporous	323:332	arg1	silicate					339:346	mesoporous zinc silicate	323:346	mesoporous zinc silicate (mZS)	323:352	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	10	3	theme	exceptional	1197:1207	arg1	features					1209:1216	exceptional features	1197:1216	exceptional features	1197:1216	Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.					
32910962	5	4	theme	dispersive	690:699	arg1	analysis					713:720	Energy dispersive x-ray (EDS) analysis	683:720	Energy dispersive x-ray (EDS) analysis	683:720	The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis.					
32910962	6	5	theme	fabricated	823:832	arg1	scaffolds					834:842	fabricated scaffolds	823:842	fabricated scaffolds	823:842	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	1	6	theme	cells	225:229	arg1	combination					179:189	a combination	177:189	a combination of scaffolds, growth factors, and cells	177:229	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	6	7	theme	Biomineralization	723:739	arg1	studies					741:747	Biomineralization studies	723:747	Biomineralization studies	723:747	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	0	8	theme	tissue	78:83	arg1	engineering					85:95	bone tissue engineering	73:95	bone tissue engineering	73:95	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
32910962	7	9	theme	antibacterial	874:886	arg1	action					888:893	antibacterial action	874:893	antibacterial action	874:893	The scaffolds also displayed antibacterial action against Staphylococcus aureus.					
32910962	9	10	theme	improved	1078:1085	arg1	porosity					1106:1113	porosity	1106:1113	porosity	1106:1113	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	10	theme	improved	1078:1085	arg1	rate					1128:1131	degradation rate	1116:1131	degradation rate	1116:1131	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	10	theme	improved	1078:1085	arg1	biomineralization					1138:1154	biomineralization	1138:1154	biomineralization	1138:1154	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	10	theme	improved	1078:1085	arg1	properties					1087:1096	matrix improved properties	1071:1096	matrix improved properties such as porosity, degradation rate, and biomineralization	1071:1154	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	11	theme	mZS	1037:1039	arg1	Incorporation					1020:1032	Incorporation	1020:1032	Incorporation of mZS within the chitosan	1020:1059	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	10	12	theme	fabricated	1168:1177	arg1	scaffolds					1179:1187	fabricated scaffolds	1168:1187	fabricated scaffolds	1168:1187	Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.					
32910962	5	13	theme	mZS	659:661	arg1	incorporation					642:654	The incorporation	638:654	The incorporation of mZS	638:661	The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis.					
32910962	6	14	theme	indicated	798:806	arg1	bioactivity					808:818	indicated bioactivity	798:818	indicated bioactivity of fabricated scaffolds	798:842	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	10	15	theme	BTE	1268:1270	arg1	applications					1272:1283	BTE applications	1268:1283	BTE applications	1268:1283	Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.					
32910962	8	16	theme	Cellular	926:933	arg1	attachment					935:944	Cellular attachment	926:944	Cellular attachment within the scaffold network	926:972	Cellular attachment within the scaffold network established biocompatibility of the material.					
32910962	4	17	theme	Emission	590:597	arg1	Microscopy					617:626	Field Emission Scanning Electron Microscopy	584:626	Field Emission Scanning Electron Microscopy (FE-SEM)	584:635	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	4	17	theme	Emission	590:597	arg1	FE-SEM					629:634	FE-SEM	629:634	FE-SEM	629:634	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	2	18	theme	engineering	408:418	arg1	applications					426:437	bone tissue engineering (BTE) applications	396:437	bone tissue engineering (BTE) applications	396:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	5	19	theme	x-ray	701:705	arg1	analysis					713:720	Energy dispersive x-ray (EDS) analysis	683:720	Energy dispersive x-ray (EDS) analysis	683:720	The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis.					
32910962	2	20	theme	tissue	401:406	arg1	BTE					421:423	BTE	421:423	BTE	421:423	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	20	theme	tissue	401:406	arg1	engineering					408:418	bone tissue engineering	396:418	bone tissue engineering (BTE) applications	396:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	1	21	theme	tissues	166:172	arg1	repair					138:143	repair	138:143	repair	138:143	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	1	21	theme	tissues	166:172	arg1	regeneration					150:161	regeneration	150:161	regeneration	150:161	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	1	21	theme	tissues	166:172	arg1	replacement					125:135	replacement	125:135	replacement	125:135	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	2	22	theme	bone	396:399	arg1	BTE					421:423	BTE	421:423	BTE	421:423	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	22	theme	bone	396:399	arg1	engineering					408:418	bone tissue engineering	396:418	bone tissue engineering (BTE) applications	396:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	4	23	theme	Field	584:588	arg1	Microscopy					617:626	Field Emission Scanning Electron Microscopy	584:626	Field Emission Scanning Electron Microscopy (FE-SEM)	584:635	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	4	23	theme	Field	584:588	arg1	FE-SEM					629:634	FE-SEM	629:634	FE-SEM	629:634	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	8	24	theme	scaffold	957:964	arg1	network					966:972	the scaffold network	953:972	the scaffold network	953:972	Cellular attachment within the scaffold network established biocompatibility of the material.					
32910962	5	25	theme	EDS	708:710	arg1	analysis					713:720	Energy dispersive x-ray (EDS) analysis	683:720	Energy dispersive x-ray (EDS) analysis	683:720	The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis.					
32910962	9	26	theme	matrix	1071:1076	arg1	porosity					1106:1113	porosity	1106:1113	porosity	1106:1113	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	26	theme	matrix	1071:1076	arg1	rate					1128:1131	degradation rate	1116:1131	degradation rate	1116:1131	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	26	theme	matrix	1071:1076	arg1	biomineralization					1138:1154	biomineralization	1138:1154	biomineralization	1138:1154	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	9	26	theme	matrix	1071:1076	arg1	properties					1087:1096	matrix improved properties	1071:1096	matrix improved properties such as porosity, degradation rate, and biomineralization	1071:1154	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	4	27	theme	bio-composite	538:550	arg1	scaffolds					552:560	The bio-composite scaffolds	534:560	The bio-composite scaffolds	534:560	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	2	28	theme	properties	381:390	arg1	mZS					349:351	mZS	349:351	mZS	349:351	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	28	theme	properties	381:390	arg1	assessment					358:367	assessment	358:367	assessment of in vitro properties for bone tissue engineering (BTE) applications	358:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	28	theme	properties	381:390	arg1	silicate					339:346	mesoporous zinc silicate	323:346	mesoporous zinc silicate (mZS)	323:352	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	1	29	theme	Tissue	98:103	arg1	engineering					105:115	Tissue engineering	98:115	Tissue engineering	98:115	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	6	30	theme	scaffolds	834:842	arg1	fluid					782:786	simulated body fluid	767:786	simulated body fluid (SBF)	767:792	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	6	30	theme	scaffolds	834:842	arg1	bioactivity					808:818	indicated bioactivity	798:818	indicated bioactivity of fabricated scaffolds	798:842	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	6	30	theme	scaffolds	834:842	arg1	SBF					789:791	SBF	789:791	SBF	789:791	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	2	31	theme	in	372:373	arg1	properties					381:390	in vitro properties	372:390	in vitro properties	372:390	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	0	32	theme	zinc	26:29	arg1	evaluation					12:21	An in vitro evaluation	0:21	An in vitro evaluation of zinc	0:29	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
32910962	10	33	contain	have	1222:1225	arg1	scaffolds					1179:1187	fabricated scaffolds	1168:1187	fabricated scaffolds	1168:1187	Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.					
32910962	10	33	contain	have	1222:1225	arg2	potential					1231:1239	the potential to serve as an implant for BTE applications	1227:1283	the potential to serve as an implant for BTE applications	1227:1283	Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.					
32910962	8	34	theme	material	1010:1017	arg1	biocompatibility					986:1001	biocompatibility	986:1001	biocompatibility of the material	986:1017	Cellular attachment within the scaffold network established biocompatibility of the material.					
32910962	1	35	theme	scaffolds	194:202	arg1	combination					179:189	a combination	177:189	a combination of scaffolds, growth factors, and cells	177:229	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	0	36	theme	fortified	40:48	arg1	scaffolds					59:67	fortified chitosan scaffolds	40:67	fortified chitosan scaffolds for bone tissue engineering	40:95	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
32910962	6	37	theme	body	777:780	arg1	fluid					782:786	simulated body fluid	767:786	simulated body fluid (SBF)	767:792	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	6	37	theme	body	777:780	arg1	SBF					789:791	SBF	789:791	SBF	789:791	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	2	38	theme	zinc	334:337	arg1	mZS					349:351	mZS	349:351	mZS	349:351	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	38	theme	zinc	334:337	arg1	silicate					339:346	mesoporous zinc silicate	323:346	mesoporous zinc silicate (mZS)	323:352	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	6	39	theme	simulated	767:775	arg1	fluid					782:786	simulated body fluid	767:786	simulated body fluid (SBF)	767:792	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	6	39	theme	simulated	767:775	arg1	SBF					789:791	SBF	789:791	SBF	789:791	Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds.					
32910962	2	40	dep	in	372:373	arg1	vitro					375:379	vitro	375:379	vitro	375:379	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	2	41	theme	scaffolds	298:306	arg1	synthesis					261:269	the synthesis	257:269	the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications	257:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	4	42	theme	Scanning	599:606	arg1	Microscopy					617:626	Field Emission Scanning Electron Microscopy	584:626	Field Emission Scanning Electron Microscopy (FE-SEM)	584:635	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	4	42	theme	Scanning	599:606	arg1	FE-SEM					629:634	FE-SEM	629:634	FE-SEM	629:634	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	2	43	theme	composite	288:296	arg1	scaffolds					298:306	biodegradable composite scaffolds	274:306	biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications	274:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	9	44	theme	degradation	1116:1126	arg1	rate					1128:1131	degradation rate	1116:1131	degradation rate	1116:1131	Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization.					
32910962	2	45	theme	biodegradable	274:286	arg1	scaffolds					298:306	biodegradable composite scaffolds	274:306	biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications	274:437	In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications.					
32910962	1	46	theme	growth	205:210	arg1	factors					212:218	growth factors	205:218	growth factors	205:218	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	0	47	dep	in	3:4	arg1	vitro					6:10	vitro	6:10	vitro	6:10	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
32910962	4	48	theme	Electron	608:615	arg1	Microscopy					617:626	Field Emission Scanning Electron Microscopy	584:626	Field Emission Scanning Electron Microscopy (FE-SEM)	584:635	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	4	48	theme	Electron	608:615	arg1	FE-SEM					629:634	FE-SEM	629:634	FE-SEM	629:634	The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM).					
32910962	1	49	theme	factors	212:218	arg1	combination					179:189	a combination	177:189	a combination of scaffolds, growth factors, and cells	177:229	Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells.					
32910962	0	50	theme	chitosan	50:57	arg1	scaffolds					59:67	fortified chitosan scaffolds	40:67	fortified chitosan scaffolds for bone tissue engineering	40:95	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
32910962	0	51	theme	in	3:4	arg1	evaluation					12:21	An in vitro evaluation	0:21	An in vitro evaluation of zinc	0:29	An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.					
34696603	0	0	theme	Different	109:117	arg1	Types					129:133	Different Cartilage Types	109:133	Different Cartilage Types	109:133	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	6	1	with	regions	1073:1079	arg1	concentrations					1097:1110	higher GAG concentrations	1086:1110	higher GAG concentrations highlighted by the contrast agent	1086:1144	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	5	2	theme	0.79	869:872	arg1	value					860:864	an R2 value	854:864	an R2 value of 0.79 for all cartilage types	854:896	Total GAG content per volume correlates to microCT attenuation with an R2 value of 0.79 for all cartilage types.					
34696603	2	3	theme	diffusion	371:379	arg1	kinetics					381:388	The diffusion kinetics	367:388	The diffusion kinetics of CA4+ in different native cartilage types	367:432	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	6	4	theme	spatial	953:959	arg1	distribution					965:976	spatial GAG distribution	953:976	spatial GAG distribution	953:976	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	6	5	theme	contrast	1131:1138	arg1	agent					1140:1144	the contrast agent	1127:1144	the contrast agent	1127:1144	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	6	6	theme	distribution	965:976	arg1	images					943:948	Three-dimensional contrast-enhanced microCT images	899:948	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution	899:976	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	7	7	theme	GAG	1233:1235	arg1	content					1237:1243	GAG content	1233:1243	GAG content	1233:1243	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	8	theme	matrix	1339:1344	arg1	development					1346:1356	matrix development	1339:1356	matrix development	1339:1356	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	6	9	theme	heterogeneous	1059:1071	arg1	regions					1073:1079	heterogeneous regions	1059:1079	heterogeneous regions with higher GAG concentrations highlighted by the contrast agent	1059:1144	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	6	10	theme	GAG	961:963	arg1	distribution					965:976	spatial GAG distribution	953:976	spatial GAG distribution	953:976	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	7	11	theme	3-dimensiona	1203:1214	arg1	distribution					1216:1227	3-dimensiona distribution	1203:1227	3-dimensiona distribution	1203:1227	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	3	12	from	glycosaminoglycans	530:547	arg1	tissues					575:581	these different tissues	559:581	these different tissues	559:581	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.					
34696603	0	13	theme	Cartilage	119:127	arg1	Types					129:133	Different Cartilage Types	109:133	Different Cartilage Types	109:133	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	0	14	from	Quantification	69:82	arg1	Types					129:133	Different Cartilage Types	109:133	Different Cartilage Types	109:133	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	7	15	theme	distribution	1216:1227	arg1	assessment					1189:1198	assessment	1189:1198	assessment of 3-dimensiona distribution and GAG content in different types of cartilage	1189:1275	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	3	16	theme	different	565:573	arg1	tissues					575:581	these different tissues	559:581	these different tissues	559:581	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.					
34696603	4	17	theme	other	763:767	arg1	types					779:783	all other cartilage types	759:783	all other cartilage types	759:783	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	5	18	theme	Total	786:790	arg1	content					796:802	Total GAG content	786:802	Total GAG content per volume	786:813	Total GAG content per volume correlates to microCT attenuation with an R2 value of 0.79 for all cartilage types.					
34696603	7	19	theme	tissue-engineered	1400:1416	arg1	constructs					1418:1427	tissue-engineered constructs	1400:1427	time as well as tissue-engineered constructs	1384:1427	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	2	20	theme	native	411:416	arg1	types					428:432	different native cartilage types	401:432	different native cartilage types	401:432	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	5	21	theme	GAG	792:794	arg1	content					796:802	Total GAG content	786:802	Total GAG content per volume	786:813	Total GAG content per volume correlates to microCT attenuation with an R2 value of 0.79 for all cartilage types.					
34696603	0	22	from	Visualization	51:63	arg1	Types					129:133	Different Cartilage Types	109:133	Different Cartilage Types	109:133	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	7	23	theme	CA4+-enhanced	1159:1171	arg1	microCT					1173:1179	CONCLUSIONS CA4+-enhanced microCT	1147:1179	CONCLUSIONS CA4+-enhanced microCT	1147:1179	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	5	24	theme	R2	857:858	arg1	value					860:864	an R2 value	854:864	an R2 value of 0.79 for all cartilage types	854:896	Total GAG content per volume correlates to microCT attenuation with an R2 value of 0.79 for all cartilage types.					
34696603	1	25	theme	bovine	210:215	arg1	meniscal					228:235	bovine articular, meniscal, nasal, and auricular cartilage	210:267	meniscal	228:235	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	0	26	theme	Micro-Computed	18:31	arg1	Tomography					33:42	Contrast-Enhanced Micro-Computed Tomography	0:42	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.	0:134	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	6	27	theme	microCT	935:941	arg1	images					943:948	Three-dimensional contrast-enhanced microCT images	899:948	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution	899:976	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	7	28	theme	diagnostic	1307:1316	arg1	promise					1285:1291	promise	1285:1291	promise	1285:1291	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	28	theme	diagnostic	1307:1316	arg1	technique					1318:1326	an ex vivo diagnostic technique	1296:1326	an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs	1296:1427	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	4	29	theme	CA4+	692:695	arg1	kinetics					680:687	The diffusion kinetics	666:687	The diffusion kinetics of CA4+ in auricular cartilage	666:718	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	4	29	theme	CA4+	692:695	arg1	slower					738:743	slower	738:743	slower	738:743	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	1	30	theme	articular	217:225	arg1	meniscal					228:235	bovine articular, meniscal, nasal, and auricular cartilage	210:267	meniscal	228:235	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	0	31	theme	Contrast-Enhanced	0:16	arg1	Tomography					33:42	Contrast-Enhanced Micro-Computed Tomography	0:42	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.	0:134	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	7	32	contain	has	1281:1283	arg2	promise					1285:1291	promise	1285:1291	promise	1285:1291	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	32	contain	has	1281:1283	arg1	microCT					1173:1179	CONCLUSIONS CA4+-enhanced microCT	1147:1179	CONCLUSIONS CA4+-enhanced microCT	1147:1179	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	32	contain	has	1281:1283	arg2	technique					1318:1326	an ex vivo diagnostic technique	1296:1326	an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs	1296:1427	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	2	33	theme	CA4+	393:396	arg1	kinetics					381:388	The diffusion kinetics	367:388	The diffusion kinetics of CA4+ in different native cartilage types	367:432	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	1	34	theme	different	296:304	arg1	ECM					328:330	ECM	328:330	ECM	328:330	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	34	theme	different	296:304	arg1	matrix					320:325	a different extracellular matrix	294:325	a different extracellular matrix (ECM) composition	294:343	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	7	35	theme	CONCLUSIONS	1147:1157	arg1	microCT					1173:1179	CONCLUSIONS CA4+-enhanced microCT	1147:1179	CONCLUSIONS CA4+-enhanced microCT	1147:1179	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	2	36	from	kinetics	381:388	arg1	types					428:432	different native cartilage types	401:432	different native cartilage types	401:432	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	2	37	dep	DESIGN	360:365	arg1	assessed					439:446	assessed	439:446	were assessed over 20 hours	434:460	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	4	38	from	kinetics	680:687	arg1	cartilage					710:718	auricular cartilage	700:718	auricular cartilage	700:718	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	1	39	theme	meniscal	228:235	arg1	microCT					198:204	microCT	198:204	microCT	198:204	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	39	theme	meniscal	228:235	arg1	tomography					186:195	CA4+-enhanced micro-computed tomography	157:195	CA4+-enhanced micro-computed tomography (microCT)	157:205	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	7	40	theme	cartilage	1267:1275	arg1	types					1258:1262	different types	1248:1262	different types of cartilage	1248:1275	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	1	41	theme	extracellular	306:318	arg1	ECM					328:330	ECM	328:330	ECM	328:330	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	41	theme	extracellular	306:318	arg1	matrix					320:325	a different extracellular matrix	294:325	a different extracellular matrix (ECM) composition	294:343	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	6	42	from	differences	1024:1034	arg1	structure					1043:1051	ECM structure	1039:1051	ECM structure	1039:1051	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	6	43	theme	contrast-enhanced	917:933	arg1	images					943:948	Three-dimensional contrast-enhanced microCT images	899:948	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution	899:976	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	3	44	theme	CA4+-enhanced	482:494	arg1	microCT					496:502	CA4+-enhanced microCT	482:502	CA4+-enhanced microCT	482:502	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.					
34696603	7	45	theme	different	1248:1256	arg1	types					1258:1262	different types	1248:1262	different types of cartilage	1248:1275	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	1	46	theme	matrix	320:325	arg1	composition					333:343	a different extracellular matrix (ECM) composition	294:343	a different extracellular matrix (ECM) composition	294:343	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	6	47	theme	Three-dimensional	899:915	arg1	images					943:948	Three-dimensional contrast-enhanced microCT images	899:948	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution	899:976	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	4	48	theme	auricular	700:708	arg1	cartilage					710:718	auricular cartilage	700:718	auricular cartilage	700:718	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	1	49	theme	nasal	238:242	arg1	meniscal					228:235	bovine articular, meniscal, nasal, and auricular cartilage	210:267	meniscal	228:235	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	0	50	theme	3D	48:49	arg1	Visualization					51:63	3D Visualization	48:63	3D Visualization	48:63	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	5	51	theme	microCT	829:835	arg1	attenuation					837:847	microCT attenuation	829:847	microCT attenuation	829:847	Total GAG content per volume correlates to microCT attenuation with an R2 value of 0.79 for all cartilage types.					
34696603	6	52	theme	higher	1086:1091	arg1	concentrations					1097:1110	higher GAG concentrations	1086:1110	higher GAG concentrations highlighted by the contrast agent	1086:1144	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	2	53	theme	cartilage	418:426	arg1	types					428:432	different native cartilage types	401:432	different native cartilage types	401:432	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	7	54	theme	content	1237:1243	arg1	assessment					1189:1198	assessment	1189:1198	assessment of 3-dimensiona distribution and GAG content in different types of cartilage	1189:1275	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	6	55	theme	ECM	1039:1041	arg1	structure					1043:1051	ECM structure	1039:1051	ECM structure	1039:1051	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	3	56	theme	safranin-O	600:609	arg1	staining					611:618	safranin-O staining	600:618	safranin-O staining	600:618	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.					
34696603	3	57	theme	microCT	496:502	arg1	feasibility					467:477	The feasibility	463:477	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues	463:581	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.					
34696603	4	58	dep	RESULTS	658:664	arg1	slower					738:743	slower	738:743	slower	738:743	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	4	58	dep	RESULTS	658:664	arg1	kinetics					680:687	The diffusion kinetics	666:687	The diffusion kinetics of CA4+ in auricular cartilage	666:718	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	4	59	theme	cartilage	769:777	arg1	types					779:783	all other cartilage types	759:783	all other cartilage types	759:783	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	1	60	contain	possesses	284:292	arg2	composition					333:343	a different extracellular matrix (ECM) composition	294:343	a different extracellular matrix (ECM) composition	294:343	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	60	contain	possesses	284:292	arg1	microCT					198:204	microCT	198:204	microCT	198:204	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	60	contain	possesses	284:292	arg1	tomography					186:195	CA4+-enhanced micro-computed tomography	157:195	CA4+-enhanced micro-computed tomography (microCT)	157:205	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	60	contain	possesses	284:292	arg2	structure					349:357	structure	349:357	structure	349:357	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	60	contain	possesses	284:292	arg1	each					270:273	each	270:273	each	270:273	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	6	61	theme	GAG	1093:1095	arg1	concentrations					1097:1110	higher GAG concentrations	1086:1110	higher GAG concentrations highlighted by the contrast agent	1086:1144	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	1	62	theme	CA4+-enhanced	157:169	arg1	microCT					198:204	microCT	198:204	microCT	198:204	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	62	theme	CA4+-enhanced	157:169	arg1	tomography					186:195	CA4+-enhanced micro-computed tomography	157:195	CA4+-enhanced micro-computed tomography (microCT)	157:205	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	7	63	dep	ex	1299:1300	arg1	vivo					1302:1305	vivo	1302:1305	vivo	1302:1305	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	1	64	theme	micro-computed	171:184	arg1	microCT					198:204	microCT	198:204	microCT	198:204	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	64	theme	micro-computed	171:184	arg1	tomography					186:195	CA4+-enhanced micro-computed tomography	157:195	CA4+-enhanced micro-computed tomography (microCT)	157:205	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	65	theme	auricular	249:257	arg1	cartilage					259:267	bovine articular, meniscal, nasal, and auricular cartilage	210:267	cartilage	259:267	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	0	66	theme	Glycosaminoglycans	87:104	arg1	Visualization					51:63	3D Visualization	48:63	3D Visualization	48:63	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	0	66	theme	Glycosaminoglycans	87:104	arg1	Quantification					69:82	Quantification	69:82	Quantification	69:82	Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.					
34696603	5	67	theme	cartilage	882:890	arg1	types					892:896	all cartilage types	878:896	all cartilage types	878:896	Total GAG content per volume correlates to microCT attenuation with an R2 value of 0.79 for all cartilage types.					
34696603	3	68	theme	blue	646:649	arg1	assay					651:655	1,9-dimethylmethylene blue assay	624:655	1,9-dimethylmethylene blue assay	624:655	The feasibility of CA4+-enhanced microCT to visualize and quantify glycosaminoglycans (GAGs) in these different tissues was tested using safranin-O staining and 1,9-dimethylmethylene blue assay.					
34696603	4	69	theme	diffusion	670:678	arg1	kinetics					680:687	The diffusion kinetics	666:687	The diffusion kinetics of CA4+ in auricular cartilage	666:718	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	4	69	theme	diffusion	670:678	arg1	slower					738:743	slower	738:743	slower	738:743	RESULTS The diffusion kinetics of CA4+ in auricular cartilage are significantly slower compared with all other cartilage types.					
34696603	2	70	theme	different	401:409	arg1	types					428:432	different native cartilage types	401:432	different native cartilage types	401:432	DESIGN The diffusion kinetics of CA4+ in different native cartilage types were assessed over 20 hours.					
34696603	1	71	theme	cartilage	259:267	arg1	microCT					198:204	microCT	198:204	microCT	198:204	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	1	71	theme	cartilage	259:267	arg1	tomography					186:195	CA4+-enhanced micro-computed tomography	157:195	CA4+-enhanced micro-computed tomography (microCT)	157:205	OBJECTIVE To compare CA4+-enhanced micro-computed tomography (microCT) of bovine articular, meniscal, nasal, and auricular cartilage, each of which possesses a different extracellular matrix (ECM) composition and structure.					
34696603	6	72	theme	safranin-O	986:995	arg1	staining					997:1004	safranin-O staining	986:1004	safranin-O staining	986:1004	Three-dimensional contrast-enhanced microCT images of spatial GAG distribution reflect safranin-O staining and highlight the differences in ECM structure, with heterogeneous regions with higher GAG concentrations highlighted by the contrast agent.					
34696603	7	73	theme	different	1361:1369	arg1	tissues					1371:1377	different tissues	1361:1377	different tissues	1361:1377	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	74	from	assessment	1189:1198	arg1	types					1258:1262	different types	1248:1262	different types of cartilage	1248:1275	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	75	theme	ex	1299:1300	arg1	promise					1285:1291	promise	1285:1291	promise	1285:1291	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34696603	7	75	theme	ex	1299:1300	arg1	technique					1318:1326	an ex vivo diagnostic technique	1296:1326	an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs	1296:1427	CONCLUSIONS CA4+-enhanced microCT enables assessment of 3-dimensiona distribution and GAG content in different types of cartilage and has promise as an ex vivo diagnostic technique to monitor matrix development in different tissues over time as well as tissue-engineered constructs.					
34894912	3	0	theme	scaffolds	388:396	arg1	form					376:379	Cryogel form	368:379	Cryogel form of the scaffolds	368:396	Cryogel form of the scaffolds created an effect that was mostly dominated by chitosan activity.					
34894912	8	1	theme	powder	1231:1236	arg1	forms					1246:1250	the powder vs. gel forms	1227:1250	forms	1246:1250	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	7	2	theme	LPS	1082:1084	arg1	macrophages					1096:1106	LPS activated macrophages	1082:1106	LPS activated macrophages	1082:1106	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	8	3	dep	assess	1220:1225	arg1	affect					1386:1391	affect	1386:1391	might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation	1380:1803	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	7	4	theme	production	1061:1070	arg1	levels					1072:1077	TNFα production levels	1056:1077	TNFα production levels by LPS activated macrophages	1056:1106	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	1	5	theme	tissue	205:210	arg1	field					224:228	the tissue engineering field	201:228	the tissue engineering field	201:228	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	8	6	theme	gel	1242:1244	arg1	forms					1246:1250	the powder vs. gel forms	1227:1250	forms	1246:1250	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	8	7	theme	gelatin	1575:1581	arg1	content					1583:1589	gelatin content	1575:1589	gelatin content	1575:1589	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	4	8	theme	chitosan	485:492	arg1	independent					470:480	independent	470:480	independent	470:480	Since independent of chitosan to gelatine ratio, the cryogels eliminated the inflammatory cytokine production by the activated macrophages.					
34894912	8	9	theme	activated	1441:1449	arg1	cryogels					1500:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	4	10	theme	cytokine	554:561	arg1	production					563:572	the inflammatory cytokine production	537:572	the inflammatory cytokine production by the activated macrophages	537:601	Since independent of chitosan to gelatine ratio, the cryogels eliminated the inflammatory cytokine production by the activated macrophages.					
34894912	1	11	from	scaffolds	188:196	arg1	field					224:228	the tissue engineering field	201:228	the tissue engineering field	201:228	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	6	12	theme	materials	815:823	arg1	effect					799:804	the immunomodulatory effect	778:804	the immunomodulatory effect of these materials	778:823	Determining the immunomodulatory effect of these materials during the decay is crucial to assess their biocompatibility and safety.					
34894912	4	13	theme	inflammatory	541:552	arg1	production					563:572	the inflammatory cytokine production	537:572	the inflammatory cytokine production by the activated macrophages	537:601	Since independent of chitosan to gelatine ratio, the cryogels eliminated the inflammatory cytokine production by the activated macrophages.					
34894912	7	14	theme	TNFα	1056:1059	arg1	levels					1072:1077	TNFα production levels	1056:1077	TNFα production levels by LPS activated macrophages	1056:1106	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	8	15	theme	composite	1490:1498	arg1	cryogels					1500:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	1	16	theme	engineering	212:222	arg1	field					224:228	the tissue engineering field	201:228	the tissue engineering field	201:228	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	7	17	contain	had	994:996	arg1	materials					984:992	the materials	980:992	the materials	980:992	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	7	17	contain	had	994:996	arg2	activity					1016:1023	anti-inflammatory activity	998:1023	anti-inflammatory activity in their powder forms	998:1045	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	8	18	theme	macrophages.HIGHLIGHTSChitosan/gelatin	1451:1488	arg1	cryogels					1500:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	0	19	theme	anti-inflammatory	13:29	arg1	properties					31:40	Differential anti-inflammatory properties	0:40	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds	0:76	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio.					
34894912	5	20	theme	matrix	728:733	arg1	nest					716:719	a nest	714:719	a nest of the matrix for the macrophages to reside	714:763	This will enable suppression of inflammatory reactions by macrophages during implant procedure while enabling a nest of the matrix for the macrophages to reside.					
34894912	8	21	from	effects	1426:1432	arg1	cryogels					1500:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1437:1507	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	1	22	theme	Chitosan/gelatine-based	115:137	arg1	materials					139:147	Chitosan/gelatine-based materials	115:147	Chitosan/gelatine-based materials	115:147	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	1	22	theme	Chitosan/gelatine-based	115:137	arg1	scaffolds					188:196	biocompatible scaffolds	174:196	biocompatible scaffolds in the tissue engineering field	174:228	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	8	23	theme	tissue	1696:1701	arg1	engineering					1703:1713	tissue engineering	1696:1713	tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation	1696:1803	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	0	24	theme	Differential	0:11	arg1	properties					31:40	Differential anti-inflammatory properties	0:40	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds	0:76	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio.					
34894912	5	25	theme	implant	681:687	arg1	procedure					689:697	implant procedure	681:697	implant procedure	681:697	This will enable suppression of inflammatory reactions by macrophages during implant procedure while enabling a nest of the matrix for the macrophages to reside.					
34894912	6	26	theme	immunomodulatory	782:797	arg1	effect					799:804	the immunomodulatory effect	778:804	the immunomodulatory effect of these materials	778:823	Determining the immunomodulatory effect of these materials during the decay is crucial to assess their biocompatibility and safety.					
34894912	7	27	theme	powder	1034:1039	arg1	forms					1041:1045	their powder forms	1028:1045	their powder forms	1028:1045	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	1	28	used	used	166:169	arg2	materials					139:147	Chitosan/gelatine-based materials	115:147	Chitosan/gelatine-based materials	115:147	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	1	28	used	used	166:169	arg2	scaffolds					188:196	biocompatible scaffolds	174:196	biocompatible scaffolds in the tissue engineering field	174:228	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	7	29	dep	had	994:996	arg1	whereas					1109:1115	whereas	1109:1115	whereas	1109:1115	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	3	30	theme	chitosan	445:452	arg1	activity					454:461	chitosan activity	445:461	chitosan activity	445:461	Cryogel form of the scaffolds created an effect that was mostly dominated by chitosan activity.					
34894912	8	31	theme	anti-inflammatory	1514:1530	arg1	ratios					1553:1558	anti-inflammatory activities.Different ratios	1514:1558	anti-inflammatory activities.Different ratios of chitosan to gelatin content	1514:1589	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	7	32	theme	chitosan	932:939	arg1	higher					951:956	higher	951:956	higher	951:956	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	7	32	theme	chitosan	932:939	arg1	ratio					941:945	the chitosan ratio	928:945	the chitosan ratio	928:945	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	0	33	theme	cryogel	60:66	arg1	scaffolds					68:76	chitosan-based cryogel scaffolds	45:76	chitosan-based cryogel scaffolds	45:76	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio.					
34894912	8	34	dep	altered	1591:1597	arg1	used					1666:1669	used	1666:1669	altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation	1591:1803	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	8	35	theme	immunomodulatory	1603:1618	arg1	activities.They					1620:1634	the immunomodulatory activities.They	1599:1634	the immunomodulatory activities.They	1599:1634	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	8	36	dep	materials	1399:1407	arg1	have					1509:1512	have	1509:1512	have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation	1509:1803	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	0	37	theme	chitosan-based	45:58	arg1	scaffolds					68:76	chitosan-based cryogel scaffolds	45:76	chitosan-based cryogel scaffolds	45:76	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio.					
34894912	8	38	theme	anti-inflammatory	1761:1777	arg1	drugs					1779:1783	anti-inflammatory drugs	1761:1783	anti-inflammatory drugs	1761:1783	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	7	39	theme	higher	1117:1122	arg1	gelatine					1124:1131	higher gelatine	1117:1131	higher gelatine to chitosan ratio	1117:1149	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	8	40	theme	chitosan	1359:1366	arg1	ratio					1350:1354	the ratio	1346:1354	the ratio of chitosan to gelatine	1346:1378	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	2	41	theme	macrophages	282:292	arg1	activities					268:277	the inflammatory activities	251:277	the inflammatory activities of macrophages	251:292	Chitosan suppresses the inflammatory activities of macrophages whereas gelatine induces inflammatory cytokine production by these cells.					
34894912	5	42	theme	inflammatory	636:647	arg1	reactions					649:657	inflammatory reactions	636:657	inflammatory reactions	636:657	This will enable suppression of inflammatory reactions by macrophages during implant procedure while enabling a nest of the matrix for the macrophages to reside.					
34894912	0	43	theme	scaffolds	68:76	arg1	properties					31:40	Differential anti-inflammatory properties	0:40	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds	0:76	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio.					
34894912	8	44	theme	implant	1674:1680	arg1	materials					1682:1690	implant materials	1674:1690	implant materials	1674:1690	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	4	45	theme	gelatine	497:504	arg1	ratio					506:510	gelatine ratio	497:510	gelatine ratio	497:510	Since independent of chitosan to gelatine ratio, the cryogels eliminated the inflammatory cytokine production by the activated macrophages.					
34894912	8	46	theme	chitosan	1563:1570	arg1	ratios					1553:1558	anti-inflammatory activities.Different ratios	1514:1558	anti-inflammatory activities.Different ratios of chitosan to gelatin content	1514:1589	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	5	47	theme	reactions	649:657	arg1	suppression					621:631	suppression	621:631	suppression of inflammatory reactions by macrophages	621:672	This will enable suppression of inflammatory reactions by macrophages during implant procedure while enabling a nest of the matrix for the macrophages to reside.					
34894912	8	48	theme	immunomodulatory	1303:1318	arg1	potentials					1320:1329	their immunomodulatory potentials	1297:1329	their immunomodulatory potentials	1297:1329	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	8	49	theme	drugs	1779:1783	arg1	use					1754:1756	the use	1750:1756	the use of anti-inflammatory drugs	1750:1783	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	3	50	theme	Cryogel	368:374	arg1	form					376:379	Cryogel form	368:379	Cryogel form of the scaffolds	368:396	Cryogel form of the scaffolds created an effect that was mostly dominated by chitosan activity.					
34894912	2	51	theme	inflammatory	255:266	arg1	activities					268:277	the inflammatory activities	251:277	the inflammatory activities of macrophages	251:292	Chitosan suppresses the inflammatory activities of macrophages whereas gelatine induces inflammatory cytokine production by these cells.					
34894912	8	52	theme	first	1205:1209	arg1	this					1193:1196	this	1193:1196	this	1193:1196	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	8	52	theme	first	1205:1209	arg1	study					1211:1215	the first study	1201:1215	the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation	1201:1803	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	7	53	from	activity	1016:1023	arg1	forms					1041:1045	their powder forms	1028:1045	their powder forms	1028:1045	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	7	54	theme	chitosan	1136:1143	arg1	ratio					1145:1149	chitosan ratio	1136:1149	chitosan ratio	1136:1149	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	2	55	theme	cytokine	332:339	arg1	production					341:350	inflammatory cytokine production	319:350	inflammatory cytokine production	319:350	Chitosan suppresses the inflammatory activities of macrophages whereas gelatine induces inflammatory cytokine production by these cells.					
34894912	7	56	theme	activated	1086:1094	arg1	macrophages					1096:1106	LPS activated macrophages	1082:1106	LPS activated macrophages	1082:1106	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	1	57	theme	biocompatible	174:186	arg1	materials					139:147	Chitosan/gelatine-based materials	115:147	Chitosan/gelatine-based materials	115:147	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	1	57	theme	biocompatible	174:186	arg1	scaffolds					188:196	biocompatible scaffolds	174:196	biocompatible scaffolds in the tissue engineering field	174:228	Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field.					
34894912	0	58	theme	chitosan/gelatin	91:106	arg1	ratio					108:112	chitosan/gelatin ratio	91:112	chitosan/gelatin ratio	91:112	Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio.					
34894912	8	59	theme	activities.Different	1532:1551	arg1	ratios					1553:1558	anti-inflammatory activities.Different ratios	1514:1558	anti-inflammatory activities.Different ratios of chitosan to gelatin content	1514:1589	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	2	60	theme	inflammatory	319:330	arg1	production					341:350	inflammatory cytokine production	319:350	inflammatory cytokine production	319:350	Chitosan suppresses the inflammatory activities of macrophages whereas gelatine induces inflammatory cytokine production by these cells.					
34894912	8	61	theme	immunomodulatory	1409:1424	arg1	effects					1426:1432	immunomodulatory effects	1409:1432	immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels	1409:1507	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	7	62	theme	anti-inflammatory	998:1014	arg1	activity					1016:1023	anti-inflammatory activity	998:1023	anti-inflammatory activity in their powder forms	998:1045	Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect.					
34894912	8	63	dep	engineering	1703:1713	arg1	reduce					1743:1748	reduce	1743:1748	will also reduce the use of anti-inflammatory drugs during implantation	1733:1803	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	4	64	theme	activated	581:589	arg1	macrophages					591:601	the activated macrophages	577:601	the activated macrophages	577:601	Since independent of chitosan to gelatine ratio, the cryogels eliminated the inflammatory cytokine production by the activated macrophages.					
34894912	8	65	theme	materials	1283:1291	arg1	forms					1246:1250	the powder vs. gel forms	1227:1250	forms	1246:1250	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
34894912	8	66	theme	chitosan/gelatine-based	1259:1281	arg1	materials					1283:1291	the chitosan/gelatine-based materials	1255:1291	the chitosan/gelatine-based materials for their immunomodulatory potentials	1255:1329	To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages.HIGHLIGHTSChitosan/gelatin composite cryogels have anti-inflammatory activities.Different ratios of chitosan to gelatin content altered the immunomodulatory activities.They can be safely and effectively used as implant materials for tissue engineering applications.They will also reduce the use of anti-inflammatory drugs during implantation.					
33892038	0	0	theme	potential	98:106	arg1	use					108:110	potential use	98:110	potential use as active food packaging materials	98:145	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	10	1	theme	compounds	1328:1336	arg1	addition					1295:1302	The addition	1291:1302	The addition of A. campestris active compounds, particularly extracts, to chitosan films	1291:1378	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	5	2	theme	FTIR	742:745	arg1	analysis					760:767	The FTIR spectroscopy analysis	738:767	The FTIR spectroscopy analysis	738:767	The FTIR spectroscopy analysis revealed covalent interaction and hydrogen bonding between chitosan and ACHE.					
33892038	10	3	theme	barrier	1425:1431	arg1	properties					1433:1442	the antioxidant and UV-Vis barrier properties	1398:1442	the antioxidant and UV-Vis barrier properties	1398:1442	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	0	4	contain	carrying	51:58	arg1	films					45:49	chitosan films	36:49	chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials	36:145	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	0	4	contain	carrying	51:58	arg2	antioxidants					81:92	Artemisia campestris antioxidants	60:92	Artemisia campestris antioxidants	60:92	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	9	5	theme	great	1225:1229	arg1	stability					1239:1247	great thermal stability	1225:1247	great thermal stability	1225:1247	All films exhibited great thermal stability as the degradation occurred above 300 °C.					
33892038	11	6	theme	antioxidant	1492:1502	arg1	compounds					1504:1512	the A. campestris antioxidant compounds	1474:1512	the A. campestris antioxidant compounds	1474:1512	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	1	7	theme	aqueous	264:270	arg1	ACAE					281:284	ACAE	281:284	ACAE	281:284	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	7	theme	aqueous	264:270	arg1	extract					272:278	aqueous extract	264:278	aqueous extract (ACAE)	264:285	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	8	8	theme	film	1143:1146	arg1	extensibility					1148:1160	The film extensibility	1139:1160	The film extensibility	1139:1160	The film extensibility was reduced when ACHE and ACEO were added.					
33892038	10	9	theme	UV-Vis	1418:1423	arg1	properties					1433:1442	the antioxidant and UV-Vis barrier properties	1398:1442	the antioxidant and UV-Vis barrier properties	1398:1442	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	3	10	theme	films	505:509	arg1	properties					487:496	The structural properties	472:496	The structural properties of the films	472:509	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	4	11	theme	films	731:735	arg1	resistance					708:717	the water resistance	698:717	the water resistance of chitosan films	698:735	The results showed that adding ACHE and ACEO improved the water resistance of chitosan films.					
33892038	3	12	theme	FTIR	560:563	arg1	spectroscopy					566:577	(FTIR) spectroscopy	559:577	(FTIR) spectroscopy	559:577	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	7	13	theme	chitosan	1091:1098	arg1	matrix					1100:1105	the chitosan matrix	1087:1105	the chitosan matrix	1087:1105	Incorporating ACHE and ACAE in the chitosan matrix decreased the tensile strength.					
33892038	4	14	theme	chitosan	722:729	arg1	films					731:735	chitosan films	722:735	chitosan films	722:735	The results showed that adding ACHE and ACEO improved the water resistance of chitosan films.					
33892038	3	15	dep	transform	540:548	arg1	infrared					550:557	infrared	550:557	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM)	540:641	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	0	16	theme	food	122:125	arg1	materials					137:145	active food packaging materials	115:145	active food packaging materials	115:145	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	4	17	theme	water	702:706	arg1	resistance					708:717	the water resistance	698:717	the water resistance of chitosan films	698:735	The results showed that adding ACHE and ACEO improved the water resistance of chitosan films.					
33892038	6	18	theme	physical	1001:1008	arg1	properties					1025:1034	the physical and mechanical properties	997:1034	the physical and mechanical properties of composite films	997:1053	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	0	19	theme	active	115:120	arg1	materials					137:145	active food packaging materials	115:145	active food packaging materials	115:145	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	6	20	theme	SEM	859:861	arg1	analyses					863:870	The XRD and SEM analyses	847:870	The XRD and SEM analyses	847:870	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	2	21	theme	films	465:469	arg1	characteristics					442:456	the physical, mechanical, thermal and antioxidant characteristics	392:456	the physical, mechanical, thermal and antioxidant characteristics of the films	392:469	The effects of incorporating A. campestris were investigated on the physical, mechanical, thermal and antioxidant characteristics of the films.					
33892038	10	22	theme	antioxidant	1402:1412	arg1	properties					1433:1442	the antioxidant and UV-Vis barrier properties	1398:1442	the antioxidant and UV-Vis barrier properties	1398:1442	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	2	23	theme	antioxidant	430:440	arg1	characteristics					442:456	the physical, mechanical, thermal and antioxidant characteristics	392:456	the physical, mechanical, thermal and antioxidant characteristics of the films	392:469	The effects of incorporating A. campestris were investigated on the physical, mechanical, thermal and antioxidant characteristics of the films.					
33892038	6	24	theme	A.	937:938	arg1	compounds					958:966	A. campestris active compounds	937:966	A. campestris active compounds	937:966	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	1	25	dep	films	170:174	arg1	enriched					198:205	enriched	198:205	enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO)	198:310	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	25	dep	films	170:174	arg1	based					176:180	based	176:180	based on chitosan	176:192	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	6	26	theme	films	1049:1053	arg1	properties					1025:1034	the physical and mechanical properties	997:1034	the physical and mechanical properties of composite films	997:1053	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	10	27	dep	A.	1307:1308	arg1	campestris					1310:1319	campestris	1310:1319	campestris	1310:1319	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	2	28	theme	campestris	360:369	arg1	effects					332:338	The effects	328:338	The effects of incorporating A. campestris	328:369	The effects of incorporating A. campestris were investigated on the physical, mechanical, thermal and antioxidant characteristics of the films.					
33892038	2	29	theme	thermal	418:424	arg1	characteristics					442:456	the physical, mechanical, thermal and antioxidant characteristics	392:456	the physical, mechanical, thermal and antioxidant characteristics of the films	392:469	The effects of incorporating A. campestris were investigated on the physical, mechanical, thermal and antioxidant characteristics of the films.					
33892038	6	30	theme	active	951:956	arg1	compounds					958:966	A. campestris active compounds	937:966	A. campestris active compounds	937:966	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	6	31	theme	mechanical	1014:1023	arg1	properties					1025:1034	the physical and mechanical properties	997:1034	the physical and mechanical properties of composite films	997:1053	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	11	32	theme	food	1534:1537	arg1	films					1454:1458	Chitosan films	1445:1458	Chitosan films enriched with the A. campestris antioxidant compounds	1445:1512	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	11	32	theme	food	1534:1537	arg1	alternatives					1549:1560	food packaging alternatives	1534:1560	food packaging alternatives	1534:1560	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	2	33	theme	mechanical	406:415	arg1	characteristics					442:456	the physical, mechanical, thermal and antioxidant characteristics	392:456	the physical, mechanical, thermal and antioxidant characteristics of the films	392:469	The effects of incorporating A. campestris were investigated on the physical, mechanical, thermal and antioxidant characteristics of the films.					
33892038	5	34	theme	hydrogen	803:810	arg1	bonding					812:818	hydrogen bonding	803:818	hydrogen bonding	803:818	The FTIR spectroscopy analysis revealed covalent interaction and hydrogen bonding between chitosan and ACHE.					
33892038	6	35	theme	campestris	940:949	arg1	compounds					958:966	A. campestris active compounds	937:966	A. campestris active compounds	937:966	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	0	36	theme	packaging	127:135	arg1	materials					137:145	active food packaging materials	115:145	active food packaging materials	115:145	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	6	37	theme	XRD	851:853	arg1	analyses					863:870	The XRD and SEM analyses	847:870	The XRD and SEM analyses	847:870	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	11	38	theme	Chitosan	1445:1452	arg1	films					1454:1458	Chitosan films	1445:1458	Chitosan films enriched with the A. campestris antioxidant compounds	1445:1512	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	11	38	theme	Chitosan	1445:1452	arg1	alternatives					1549:1560	food packaging alternatives	1534:1560	food packaging alternatives	1534:1560	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	1	39	theme	Artemisia	212:220	arg1	ACHE					257:260	ACHE	257:260	ACHE	257:260	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	39	theme	Artemisia	212:220	arg1	extract					248:254	Artemisia campestris hydroalcoholic extract	212:254	Artemisia campestris hydroalcoholic extract (ACHE)	212:261	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	0	40	theme	films	45:49	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	0	40	theme	films	45:49	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	1	41	theme	essential	291:299	arg1	ACEO					306:309	ACEO	306:309	ACEO	306:309	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	41	theme	essential	291:299	arg1	oil					301:303	essential oil	291:303	essential oil (ACEO)	291:310	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	42	theme	campestris	222:231	arg1	ACHE					257:260	ACHE	257:260	ACHE	257:260	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	42	theme	campestris	222:231	arg1	extract					248:254	Artemisia campestris hydroalcoholic extract	212:254	Artemisia campestris hydroalcoholic extract (ACHE)	212:261	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	3	43	theme	X-ray	580:584	arg1	XRD					599:601	XRD	599:601	XRD	599:601	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	3	43	theme	X-ray	580:584	arg1	diffraction					586:596	X-ray diffraction	580:596	X-ray diffraction (XRD)	580:602	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	6	44	theme	composite	1039:1047	arg1	films					1049:1053	composite films	1039:1053	composite films	1039:1053	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	2	45	theme	physical	396:403	arg1	characteristics					442:456	the physical, mechanical, thermal and antioxidant characteristics	392:456	the physical, mechanical, thermal and antioxidant characteristics of the films	392:469	The effects of incorporating A. campestris were investigated on the physical, mechanical, thermal and antioxidant characteristics of the films.					
33892038	0	46	theme	chitosan	36:43	arg1	films					45:49	chitosan films	36:49	chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials	36:145	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	5	47	theme	spectroscopy	747:758	arg1	analysis					760:767	The FTIR spectroscopy analysis	738:767	The FTIR spectroscopy analysis	738:767	The FTIR spectroscopy analysis revealed covalent interaction and hydrogen bonding between chitosan and ACHE.					
33892038	3	48	theme	scanning	608:615	arg1	microscopy					626:635	scanning electron microscopy	608:635	scanning electron microscopy (SEM)	608:641	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	3	48	theme	scanning	608:615	arg1	SEM					638:640	SEM	638:640	SEM	638:640	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	10	49	theme	active	1321:1326	arg1	compounds					1328:1336	A. campestris active compounds	1307:1336	A. campestris active compounds	1307:1336	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	10	49	theme	active	1321:1326	arg1	extracts					1352:1359	extracts	1352:1359	particularly extracts	1339:1359	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	1	50	theme	hydroalcoholic	233:246	arg1	ACHE					257:260	ACHE	257:260	ACHE	257:260	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	50	theme	hydroalcoholic	233:246	arg1	extract					248:254	Artemisia campestris hydroalcoholic extract	212:254	Artemisia campestris hydroalcoholic extract (ACHE)	212:261	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	0	51	theme	Artemisia	60:68	arg1	antioxidants					81:92	Artemisia campestris antioxidants	60:92	Artemisia campestris antioxidants	60:92	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	3	52	theme	electron	617:624	arg1	microscopy					626:635	scanning electron microscopy	608:635	scanning electron microscopy (SEM)	608:641	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	3	52	theme	electron	617:624	arg1	SEM					638:640	SEM	638:640	SEM	638:640	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	3	53	theme	structural	476:485	arg1	properties					487:496	The structural properties	472:496	The structural properties of the films	472:509	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	7	54	theme	tensile	1121:1127	arg1	strength					1129:1136	the tensile strength	1117:1136	the tensile strength	1117:1136	Incorporating ACHE and ACAE in the chitosan matrix decreased the tensile strength.					
33892038	6	55	theme	film	921:924	arg1	matrix					926:931	the film matrix	917:931	the film matrix	917:931	The XRD and SEM analyses indicated that interactions occurred between the film matrix and A. campestris active compounds, which could be reflected by the physical and mechanical properties of composite films.					
33892038	9	56	theme	thermal	1231:1237	arg1	stability					1239:1247	great thermal stability	1225:1247	great thermal stability	1225:1247	All films exhibited great thermal stability as the degradation occurred above 300 °C.					
33892038	11	57	theme	packaging	1539:1547	arg1	films					1454:1458	Chitosan films	1445:1458	Chitosan films enriched with the A. campestris antioxidant compounds	1445:1512	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	11	57	theme	packaging	1539:1547	arg1	alternatives					1549:1560	food packaging alternatives	1534:1560	food packaging alternatives	1534:1560	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	10	58	theme	A.	1307:1308	arg1	compounds					1328:1336	A. campestris active compounds	1307:1336	A. campestris active compounds	1307:1336	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	10	58	theme	A.	1307:1308	arg1	extracts					1352:1359	extracts	1352:1359	particularly extracts	1339:1359	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	11	59	theme	A.	1478:1479	arg1	compounds					1504:1512	the A. campestris antioxidant compounds	1474:1512	the A. campestris antioxidant compounds	1474:1512	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	10	60	theme	chitosan	1365:1372	arg1	films					1374:1378	chitosan films	1365:1378	chitosan films	1365:1378	The addition of A. campestris active compounds, particularly extracts, to chitosan films notably increased the antioxidant and UV-Vis barrier properties.					
33892038	3	61	dep	Fourier	532:538	arg1	transform					540:548	transform	540:548	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM)	540:641	The structural properties of the films were evaluated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM).					
33892038	11	62	theme	campestris	1481:1490	arg1	compounds					1504:1512	the A. campestris antioxidant compounds	1474:1512	the A. campestris antioxidant compounds	1474:1512	Chitosan films enriched with the A. campestris antioxidant compounds could be applied as food packaging alternatives.					
33892038	5	63	theme	covalent	778:785	arg1	interaction					787:797	covalent interaction	778:797	covalent interaction	778:797	The FTIR spectroscopy analysis revealed covalent interaction and hydrogen bonding between chitosan and ACHE.					
33892038	1	64	theme	Active	148:153	arg1	films					170:174	Active food packaging films	148:174	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO)	148:310	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	1	65	theme	food	155:158	arg1	films					170:174	Active food packaging films	148:174	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO)	148:310	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
33892038	0	66	theme	campestris	70:79	arg1	antioxidants					81:92	Artemisia campestris antioxidants	60:92	Artemisia campestris antioxidants	60:92	Development and characterization of chitosan films carrying Artemisia campestris antioxidants for potential use as active food packaging materials.					
33892038	1	67	theme	packaging	160:168	arg1	films					170:174	Active food packaging films	148:174	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO)	148:310	Active food packaging films based on chitosan and enriched with Artemisia campestris hydroalcoholic extract (ACHE), aqueous extract (ACAE) and essential oil (ACEO) were developed.					
34768900	10	0	theme	clinical	1447:1454	arg1	settings					1456:1463	distinct clinical settings	1438:1463	distinct clinical settings depending on their localization or tissue properties	1438:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	2	1	from	solubility	394:403	arg1	tissue					440:445	neuronal tissue	431:445	neuronal tissue	431:445	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	1	2	theme	several	137:143	arg1	materials					157:165	several hemostyptic materials	137:165	several hemostyptic materials	137:165	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	2	theme	several	137:143	arg1	Equicel®					187:194	Equicel®	187:194	Equicel®	187:194	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	2	theme	several	137:143	arg1	Equitamp®					200:208	Equitamp®	200:208	Equitamp®	200:208	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	2	theme	several	137:143	arg1	Tabotamp®					176:184	Tabotamp®	176:184	Tabotamp®	176:184	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	9	3	theme	Tabotamp®	1256:1264	arg1	treatment					1266:1274	Tabotamp® treatment	1256:1274	Tabotamp® treatment	1256:1274	Microglia cells were not detectable after Tabotamp® treatment, presumably due to an artifact caused by strong pH reduction.					
34768900	0	4	theme	Neuronal	73:80	arg1	Tissue					82:87	Neuronal Tissue	73:87	Neuronal Tissue	73:87	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	2	5	from	effects	420:426	arg1	tissue					440:445	neuronal tissue	431:445	neuronal tissue	431:445	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	3	6	theme	fluorescence	490:501	arg1	dye					503:505	DNA-binding fluorescence dye	478:505	DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells	478:554	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	10	7	theme	Equitamp®	1399:1407	arg1	use					1369:1371	the use	1365:1371	the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties	1365:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	0	8	from	Variants	0:7	arg1	Tissue					82:87	Neuronal Tissue	73:87	Neuronal Tissue	73:87	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	8	9	theme	p	1202:1202	arg1	<					1204:1204	p < 0.001	1202:1210	p < 0.001	1202:1210	Equicel® increased strongly the number of microglia cells after 24 h (p < 0.001).					
34768900	8	9	theme	p	1202:1202	arg1	h					1199:1199	24 h	1196:1199	24 h (p < 0.001)	1196:1211	Equicel® increased strongly the number of microglia cells after 24 h (p < 0.001).					
34768900	4	10	theme	cellular	720:727	arg1	damage					729:734	the cellular damage	716:734	the cellular damage	716:734	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	1	11	theme	hemostyptic	145:155	arg1	materials					157:165	several hemostyptic materials	137:165	several hemostyptic materials	137:165	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	11	theme	hemostyptic	145:155	arg1	Equicel®					187:194	Equicel®	187:194	Equicel®	187:194	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	11	theme	hemostyptic	145:155	arg1	Equitamp®					200:208	Equitamp®	200:208	Equitamp®	200:208	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	11	theme	hemostyptic	145:155	arg1	Tabotamp®					176:184	Tabotamp®	176:184	Tabotamp®	176:184	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	5	12	theme	significant	870:880	arg1	reduction					882:890	a significant reduction	868:890	a significant reduction of pH (p < 0.001)	868:908	Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001).					
34768900	10	13	from	applications	1422:1433	arg1	localization					1484:1495	localization	1484:1495	localization	1484:1495	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	10	13	from	applications	1422:1433	arg1	properties					1507:1516	tissue properties	1500:1516	tissue properties	1500:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	10	13	from	applications	1422:1433	arg1	settings					1456:1463	distinct clinical settings	1438:1463	distinct clinical settings depending on their localization or tissue properties	1438:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	5	14	theme	p	899:899	arg1	pH					895:896	pH	895:896	pH (p < 0.001)	895:908	Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001).					
34768900	5	14	theme	p	899:899	arg1	<					901:901	p < 0.001	899:907	p < 0.001	899:907	Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001).					
34768900	3	15	from	dye	503:505	arg1	astrocytes					525:534	astrocytes	525:534	astrocytes	525:534	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	3	15	from	dye	503:505	arg1	cells					518:522	Schwann cells	510:522	Schwann cells	510:522	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	3	15	from	dye	503:505	arg1	cells					550:554	neuronal cells	541:554	neuronal cells	541:554	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	3	16	theme	DNA-binding	478:488	arg1	dye					503:505	DNA-binding fluorescence dye	478:505	DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells	478:554	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	1	17	theme	challenging	243:253	arg1	hemostasis					255:264	challenging hemostasis	243:264	challenging hemostasis	243:264	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	10	18	theme	Tabotamp®	1386:1394	arg1	use					1369:1371	the use	1365:1371	the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties	1365:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	4	19	theme	B4	689:690	arg1	staining					692:699	isolectin B4 staining	679:699	isolectin B4 staining	679:699	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	7	20	theme	deeper	1059:1064	arg1	damage					1066:1071	a stronger and deeper damage	1044:1071	a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01)	1044:1129	On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01).					
34768900	3	21	theme	Schwann	510:516	arg1	cells					518:522	Schwann cells	510:522	Schwann cells	510:522	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	7	22	theme	neuronal	1080:1087	arg1	tissue					1089:1094	the neuronal tissue	1076:1094	the neuronal tissue	1076:1094	On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01).					
34768900	10	23	from	settings	1456:1463	arg1	localization					1484:1495	localization	1484:1495	localization	1484:1495	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	10	23	from	settings	1456:1463	arg1	properties					1507:1516	tissue properties	1500:1516	tissue properties	1500:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	4	24	dep	damage	729:734	arg1	activation					769:778	activation	769:778	activation	769:778	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	3	25	theme	neuronal	541:548	arg1	cells					550:554	neuronal cells	541:554	neuronal cells	541:554	Cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes, and neuronal cells.					
34768900	6	26	theme	p	994:994	arg1	lines					987:991	several cell lines	974:991	several cell lines (p < 0.01)	974:1002	Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01).					
34768900	6	26	theme	p	994:994	arg1	<					996:996	p < 0.01	994:1001	p < 0.01	994:1001	Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01).					
34768900	2	27	theme	properties	355:364	arg1	terms					329:333	terms	329:333	terms of compositions and properties	329:364	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	0	28	theme	Regenerated	21:31	arg1	Cellulose					33:41	Oxidized Regenerated Cellulose	12:41	Oxidized Regenerated Cellulose	12:41	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	2	29	theme	present	287:293	arg1	study					295:299	The present study	283:299	The present study	283:299	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	5	30	theme	neutral	807:813	arg1	pH					815:816	a neutral pH	805:816	a neutral pH	805:816	Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001).					
34768900	2	31	theme	pH	406:407	arg1	values					409:414	pH values	406:414	pH values	406:414	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	1	32	theme	oxidized	99:106	arg1	ORC					131:133	ORC	131:133	ORC	131:133	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	32	theme	oxidized	99:106	arg1	cellulose					120:128	oxidized regenerated cellulose	99:128	oxidized regenerated cellulose (ORC)	99:134	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	6	33	theme	cell	982:985	arg1	lines					987:991	several cell lines	974:991	several cell lines (p < 0.01)	974:1002	Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01).					
34768900	6	33	theme	cell	982:985	arg1	<					996:996	p < 0.01	994:1001	p < 0.01	994:1001	Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01).					
34768900	9	34	theme	strong	1317:1322	arg1	reduction					1327:1335	strong pH reduction	1317:1335	strong pH reduction	1317:1335	Microglia cells were not detectable after Tabotamp® treatment, presumably due to an artifact caused by strong pH reduction.					
34768900	4	35	theme	propidium	638:646	arg1	iodide					648:653	propidium iodide	638:653	propidium iodide	638:653	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	6	36	theme	several	974:980	arg1	lines					987:991	several cell lines	974:991	several cell lines (p < 0.01)	974:1002	Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01).					
34768900	6	36	theme	several	974:980	arg1	<					996:996	p < 0.01	994:1001	p < 0.01	994:1001	Equicel® and Tabotamp® increased cytotoxicity significantly in several cell lines (p < 0.01).					
34768900	0	37	theme	Cellulose	33:41	arg1	Variants					0:7	Variants	0:7	Variants of Oxidized Regenerated Cellulose	0:41	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	0	37	theme	Cellulose	33:41	arg1	Effects					62:68	Their Distinct Effects	47:68	Their Distinct Effects on Neuronal Tissue	47:87	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	7	38	theme	stronger	1046:1053	arg1	damage					1066:1071	a stronger and deeper damage	1044:1071	a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01)	1044:1129	On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01).					
34768900	10	39	theme	specific	1413:1420	arg1	applications					1422:1433	specific applications	1413:1433	specific applications in distinct clinical settings depending on their localization or tissue properties	1413:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	9	40	theme	pH	1324:1325	arg1	reduction					1327:1335	strong pH reduction	1317:1335	strong pH reduction	1317:1335	Microglia cells were not detectable after Tabotamp® treatment, presumably due to an artifact caused by strong pH reduction.					
34768900	0	41	theme	Distinct	53:60	arg1	Effects					62:68	Their Distinct Effects	47:68	Their Distinct Effects on Neuronal Tissue	47:87	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	0	42	from	Effects	62:68	arg1	Tissue					82:87	Neuronal Tissue	73:87	Neuronal Tissue	73:87	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	1	43	theme	regenerated	108:118	arg1	ORC					131:133	ORC	131:133	ORC	131:133	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	1	43	theme	regenerated	108:118	arg1	cellulose					120:128	oxidized regenerated cellulose	99:128	oxidized regenerated cellulose (ORC)	99:134	Based on oxidized regenerated cellulose (ORC), several hemostyptic materials, such as Tabotamp®, Equicel® and Equitamp®, have been developed to approach challenging hemostasis in neurosurgery.					
34768900	10	44	theme	distinct	1438:1445	arg1	settings					1456:1463	distinct clinical settings	1438:1463	distinct clinical settings depending on their localization or tissue properties	1438:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	9	45	theme	Microglia	1214:1222	arg1	cells					1224:1228	Microglia cells	1214:1228	Microglia cells	1214:1228	Microglia cells were not detectable after Tabotamp® treatment, presumably due to an artifact caused by strong pH reduction.					
34768900	4	46	theme	organotypic	571:581	arg1	OHSC					611:614	OHSC	611:614	OHSC	611:614	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	4	46	theme	organotypic	571:581	arg1	cultures					601:608	organotypic hippocampal slice cultures	571:608	organotypic hippocampal slice cultures (OHSC)	571:615	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	4	47	theme	isolectin	679:687	arg1	B4					689:690	isolectin B4	679:690	isolectin B4 staining	679:699	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	10	48	from	localization	1484:1495	arg1	applications					1422:1433	specific applications	1413:1433	specific applications in distinct clinical settings depending on their localization or tissue properties	1413:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	8	49	theme	microglia	1174:1182	arg1	cells					1184:1188	microglia cells	1174:1188	microglia cells	1174:1188	Equicel® increased strongly the number of microglia cells after 24 h (p < 0.001).					
34768900	4	50	theme	slice	595:599	arg1	OHSC					611:614	OHSC	611:614	OHSC	611:614	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	4	50	theme	slice	595:599	arg1	cultures					601:608	organotypic hippocampal slice cultures	571:608	organotypic hippocampal slice cultures (OHSC)	571:615	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	10	51	from	properties	1507:1516	arg1	applications					1422:1433	specific applications	1413:1433	specific applications in distinct clinical settings depending on their localization or tissue properties	1413:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	8	52	theme	cells	1184:1188	arg1	number					1164:1169	the number	1160:1169	the number of microglia cells	1160:1188	Equicel® increased strongly the number of microglia cells after 24 h (p < 0.001).					
34768900	4	53	theme	hippocampal	583:593	arg1	OHSC					611:614	OHSC	611:614	OHSC	611:614	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	4	53	theme	hippocampal	583:593	arg1	cultures					601:608	organotypic hippocampal slice cultures	571:608	organotypic hippocampal slice cultures (OHSC)	571:615	Additionally, organotypic hippocampal slice cultures (OHSC) were analyzed, using propidium iodide, hematoxylin-eosin, and isolectin B4 staining to investigate the cellular damage, cytoarchitecture, and microglia activation.					
34768900	10	54	theme	tissue	1500:1505	arg1	properties					1507:1516	tissue properties	1500:1516	tissue properties	1500:1516	In summary, our data imply the use of Equicel®, Tabotamp® or Equitamp® for specific applications in distinct clinical settings depending on their localization or tissue properties.					
34768900	2	55	from	structure	383:391	arg1	tissue					440:445	neuronal tissue	431:445	neuronal tissue	431:445	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	5	56	theme	pH	895:896	arg1	reduction					882:890	a significant reduction	868:890	a significant reduction of pH (p < 0.001)	868:908	Whereas Equicel® led to a neutral pH, Tabotamp® (pH 2.8) and Equitamp® (pH 4.8) caused a significant reduction of pH (p < 0.001).					
34768900	2	57	theme	neuronal	431:438	arg1	tissue					440:445	neuronal tissue	431:445	neuronal tissue	431:445	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	2	58	from	values	409:414	arg1	tissue					440:445	neuronal tissue	431:445	neuronal tissue	431:445	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	0	59	theme	Oxidized	12:19	arg1	Cellulose					33:41	Oxidized Regenerated Cellulose	12:41	Oxidized Regenerated Cellulose	12:41	Variants of Oxidized Regenerated Cellulose and Their Distinct Effects on Neuronal Tissue.					
34768900	2	60	theme	compositions	338:349	arg1	terms					329:333	terms	329:333	terms of compositions and properties	329:364	The present study compares ORC that differ in terms of compositions and properties, regarding their structure, solubility, pH values and effects on neuronal tissue.					
34768900	7	61	theme	p	1121:1121	arg1	gauze					1114:1118	gauze	1114:1118	gauze (p < 0.01)	1114:1129	On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01).					
34768900	7	61	theme	p	1121:1121	arg1	<					1123:1123	p < 0.01	1121:1128	p < 0.01	1121:1128	On OHSC, Tabotamp® and Equicel® led to a stronger and deeper damage to the neuronal tissue than Equitamp® or gauze (p < 0.01).					
32594109	8	0	theme	warfarin-treated	1107:1122	arg1	individuals					1124:1134	the warfarin-treated individuals	1103:1134	the warfarin-treated individuals	1103:1134	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	10	1	theme	glycan	1312:1317	arg1	types					1303:1307	Eight types	1297:1307	Eight types of glycan	1297:1317	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	7	2	dep	RESULTS	846:852	arg1	concentration					858:870	The concentration	854:870	The concentration of PIVKA-II	854:882	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	7	2	dep	RESULTS	846:852	arg1	higher					902:907	higher	902:907	higher	902:907	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	2	3	contain	have	249:252	arg2	synthesis					272:280	disturbed protein synthesis	254:280	disturbed protein synthesis	254:280	Those patients have disturbed protein synthesis and glycosylation in the liver.					
32594109	2	3	contain	have	249:252	arg1	patients					240:247	Those patients	234:247	Those patients	234:247	Those patients have disturbed protein synthesis and glycosylation in the liver.					
32594109	2	3	contain	have	249:252	arg2	glycosylation					286:298	glycosylation	286:298	glycosylation	286:298	Those patients have disturbed protein synthesis and glycosylation in the liver.					
32594109	10	4	theme	prothrombin	1468:1478	arg1	glycosylation					1451:1463	glycosylation	1451:1463	glycosylation of prothrombin	1451:1478	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	11	5	theme	Warfarin	1493:1500	arg1	therapy					1502:1508	CONCLUSIONS Warfarin therapy	1481:1508	CONCLUSIONS Warfarin therapy	1481:1508	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	0	6	from	Influence	0:8	arg1	Modifications					82:94	Its Posttranslational Modifications	60:94	Its Posttranslational Modifications	60:94	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.					
32594109	0	6	from	Influence	0:8	arg1	Production					45:54	Prothrombin Production	33:54	Prothrombin Production	33:54	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.					
32594109	11	7	theme	Gla	1552:1554	arg1	residues					1556:1563	prothrombin and Gla residues	1536:1563	residues	1556:1563	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	6	8	theme	Glycan	649:654	arg1	structures					656:665	Glycan structures	649:665	Glycan structures in the purified prothrombin containing PIVKA-II	649:713	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	3	9	theme	residues	367:374	arg1	number					333:338	the number	329:338	the number of γ-carboxyglutamyl (Gla) residues on prothrombin	329:389	This decreases the number of γ-carboxyglutamyl (Gla) residues on prothrombin, converting prothrombin into PIVKA-II.					
32594109	7	10	theme	healthy	957:963	arg1	individuals					965:975	the healthy individuals	953:975	the healthy individuals	953:975	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	10	11	gly	glycosylation	1451:1463	arg1	prothrombin					1468:1478	prothrombin	1468:1478	prothrombin	1468:1478	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	11	12	theme	synthetic	1667:1675	arg1	function					1677:1684	protein synthetic function	1659:1684	protein synthetic function	1659:1684	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	6	13	with	glycan	804:809	arg1	2-aminobenzamide					828:843	fluorophore 2-aminobenzamide	816:843	fluorophore 2-aminobenzamide	816:843	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	11	14	theme	lower	1519:1523	arg1	residues					1556:1563	prothrombin and Gla residues	1536:1563	residues	1556:1563	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	11	14	theme	lower	1519:1523	arg1	amounts					1525:1531	lower amounts	1519:1531	lower amounts of prothrombin and Gla residues within prothrombin	1519:1582	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	11	15	theme	quantitative	1617:1628	arg1	change					1630:1635	qualitative and quantitative change	1601:1635	qualitative and quantitative change in glycan profile and protein synthetic function in the liver	1601:1697	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	5	16	theme	METHODS	500:506	arg1	Prothrombin					508:518	METHODS Prothrombin	500:518	METHODS Prothrombin	500:518	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	2	17	gly	glycosylation	286:298	arg1	liver					307:311	the liver	303:311	the liver	303:311	Those patients have disturbed protein synthesis and glycosylation in the liver.					
32594109	11	18	theme	qualitative	1601:1611	arg1	change					1630:1635	qualitative and quantitative change	1601:1635	qualitative and quantitative change in glycan profile and protein synthetic function in the liver	1601:1697	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	10	19	from	generation	1363:1372	arg1	individuals					1410:1420	the warfarin-treated individuals	1389:1420	the warfarin-treated individuals	1389:1420	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	6	20	theme	performance	751:761	arg1	chromatography					770:783	high performance liquid chromatography	746:783	high performance liquid chromatography	746:783	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	8	21	dep	lower	1094:1098	arg1	P < 0.001					1169:1177	P < 0.001	1169:1177	P < 0.001	1169:1177	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	8	22	theme	prothrombin	1078:1088	arg1	concentration					1061:1073	the concentration	1057:1073	the concentration of prothrombin	1057:1088	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	8	22	theme	prothrombin	1078:1088	arg1	lower					1094:1098	lower	1094:1098	lower	1094:1098	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	1	23	theme	BACKGROUND	97:106	arg1	Protein					108:114	BACKGROUND Protein	97:114	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II)	97:157	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II) is produced by the liver during hepatoma and upon warfarin administration.					
32594109	6	24	theme	high	746:749	arg1	chromatography					770:783	high performance liquid chromatography	746:783	high performance liquid chromatography	746:783	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	11	25	theme	glycan	1640:1645	arg1	profile					1647:1653	glycan profile	1640:1653	glycan profile	1640:1653	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	8	26	theme	healthy	1148:1154	arg1	individuals					1156:1166	the healthy individuals	1144:1166	the healthy individuals	1144:1166	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	5	27	theme	protein	603:609	arg1	production					611:620	protein production	603:620	protein production	603:620	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	7	28	theme	warfarin-treated	916:931	arg1	individuals					933:943	the warfarin-treated individuals	912:943	the warfarin-treated individuals	912:943	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	0	29	theme	Therapy	22:28	arg1	Influence					0:8	Influence	0:8	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.	0:95	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.					
32594109	5	30	theme	production	611:620	arg1	function					591:598	liver function	585:598	liver function of protein production	585:620	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	9	31	theme	main	1185:1188	arg1	A2					1201:1202	A2	1201:1202	A2	1201:1202	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	9	31	theme	main	1185:1188	arg1	glycan					1190:1195	The main glycan	1181:1195	The main glycan	1181:1195	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	8	32	from	groups	1049:1054	arg1	normal					1034:1039	normal	1034:1039	normal	1034:1039	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	0	33	theme	Warfarin	13:20	arg1	Therapy					22:28	Warfarin Therapy	13:28	Warfarin Therapy	13:28	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.					
32594109	7	34	theme	PIVKA-II	875:882	arg1	concentration					858:870	The concentration	854:870	The concentration of PIVKA-II	854:882	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	7	34	theme	PIVKA-II	875:882	arg1	higher					902:907	higher	902:907	higher	902:907	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	10	35	theme	warfarin-treated	1393:1408	arg1	individuals					1410:1420	the warfarin-treated individuals	1389:1420	the warfarin-treated individuals	1389:1420	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	11	36	theme	prothrombin	1536:1546	arg1	residues					1556:1563	prothrombin and Gla residues	1536:1563	residues	1556:1563	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	9	37	dep	individuals	1240:1250	arg1	%					1263:1263	86.6 ± 4.4%	1253:1263	86.6 ± 4.4%	1253:1263	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	9	37	dep	individuals	1240:1250	arg1	%					1279:1279	85.6 ± 3.4%	1269:1279	85.6 ± 3.4%	1269:1279	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	0	38	theme	Prothrombin	33:43	arg1	Production					45:54	Prothrombin Production	33:54	Prothrombin Production	33:54	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.					
32594109	6	39	theme	liquid	763:768	arg1	chromatography					770:783	high performance liquid chromatography	746:783	high performance liquid chromatography	746:783	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	4	40	theme	conversion	452:461	arg1	mechanism					434:442	The mechanism	430:442	The mechanism of this conversion	430:461	The mechanism of this conversion, however, is not clearly understood.					
32594109	8	41	theme	protein	998:1004	arg1	production					1006:1015	protein production	998:1015	protein production in the liver	998:1028	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	9	42	from	A2	1201:1202	arg1	individuals					1240:1250	the healthy and warfarin-treated individuals	1207:1250	the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively)	1207:1294	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	11	43	theme	CONCLUSIONS	1481:1491	arg1	therapy					1502:1508	CONCLUSIONS Warfarin therapy	1481:1508	CONCLUSIONS Warfarin therapy	1481:1508	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	5	44	theme	warfarin-treated	550:565	arg1	individuals					567:577	healthy and warfarin-treated individuals	538:577	healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal	538:646	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	1	45	theme	warfarin	209:216	arg1	administration					218:231	warfarin administration	209:231	warfarin administration	209:231	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II) is produced by the liver during hepatoma and upon warfarin administration.					
32594109	10	46	from	variation	1438:1446	arg1	glycosylation					1451:1463	glycosylation	1451:1463	glycosylation of prothrombin	1451:1478	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	1	47	theme	vitamin	127:133	arg1	absence-II					137:146	vitamin K absence-II	127:146	vitamin K absence-II (PIVKA-II)	127:157	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II) is produced by the liver during hepatoma and upon warfarin administration.					
32594109	1	47	theme	vitamin	127:133	arg1	PIVKA-II					149:156	PIVKA-II	149:156	PIVKA-II	149:156	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II) is produced by the liver during hepatoma and upon warfarin administration.					
32594109	7	48	dep	higher	902:907	arg1	P< 0.001					978:985	P< 0.001	978:985	P< 0.001	978:985	RESULTS The concentration of PIVKA-II was significantly higher in the warfarin-treated individuals than in the healthy individuals (P< 0.001).					
32594109	3	49	theme	Gla	362:364	arg1	residues					367:374	γ-carboxyglutamyl (Gla) residues	343:374	γ-carboxyglutamyl (Gla) residues	343:374	This decreases the number of γ-carboxyglutamyl (Gla) residues on prothrombin, converting prothrombin into PIVKA-II.					
32594109	6	50	theme	fluorophore	816:826	arg1	2-aminobenzamide					828:843	fluorophore 2-aminobenzamide	816:843	fluorophore 2-aminobenzamide	816:843	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	1	51	theme	K	135:135	arg1	absence-II					137:146	vitamin K absence-II	127:146	vitamin K absence-II (PIVKA-II)	127:157	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II) is produced by the liver during hepatoma and upon warfarin administration.					
32594109	1	51	theme	K	135:135	arg1	PIVKA-II					149:156	PIVKA-II	149:156	PIVKA-II	149:156	BACKGROUND Protein induced by vitamin K absence-II (PIVKA-II) is produced by the liver during hepatoma and upon warfarin administration.					
32594109	11	52	theme	protein	1659:1665	arg1	function					1677:1684	protein synthetic function	1659:1684	protein synthetic function	1659:1684	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	3	53	from	number	333:338	arg1	prothrombin					379:389	prothrombin	379:389	prothrombin	379:389	This decreases the number of γ-carboxyglutamyl (Gla) residues on prothrombin, converting prothrombin into PIVKA-II.					
32594109	6	54	from	structures	656:665	arg1	PIVKA-II					706:713	the purified prothrombin containing PIVKA-II	670:713	the purified prothrombin containing PIVKA-II	670:713	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	5	55	theme	liver	585:589	arg1	function					591:598	liver function	585:598	liver function of protein production	585:620	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	6	56	theme	containing	695:704	arg1	PIVKA-II					706:713	the purified prothrombin containing PIVKA-II	670:713	the purified prothrombin containing PIVKA-II	670:713	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	9	57	theme	healthy	1211:1217	arg1	individuals					1240:1250	the healthy and warfarin-treated individuals	1207:1250	the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively)	1207:1294	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	2	58	theme	protein	264:270	arg1	synthesis					272:280	disturbed protein synthesis	254:280	disturbed protein synthesis	254:280	Those patients have disturbed protein synthesis and glycosylation in the liver.					
32594109	10	59	theme	PIVKA-II	1377:1384	arg1	generation					1363:1372	generation	1363:1372	generation of PIVKA-II in the warfarin-treated individuals	1363:1420	Eight types of glycan were characterized in both groups, although generation of PIVKA-II in the warfarin-treated individuals did not lead to variation in glycosylation of prothrombin.					
32594109	6	60	theme	prothrombin	683:693	arg1	PIVKA-II					706:713	the purified prothrombin containing PIVKA-II	670:713	the purified prothrombin containing PIVKA-II	670:713	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	11	61	theme	residues	1556:1563	arg1	residues					1556:1563	prothrombin and Gla residues	1536:1563	residues	1556:1563	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	11	61	theme	residues	1556:1563	arg1	amounts					1525:1531	lower amounts	1519:1531	lower amounts of prothrombin and Gla residues within prothrombin	1519:1582	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	0	62	theme	Posttranslational	64:80	arg1	Modifications					82:94	Its Posttranslational Modifications	60:94	Its Posttranslational Modifications	60:94	Influence of Warfarin Therapy on Prothrombin Production and Its Posttranslational Modifications.					
32594109	8	63	from	normal	1034:1039	arg1	groups					1049:1054	both groups	1044:1054	both groups	1044:1054	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	2	64	theme	disturbed	254:262	arg1	synthesis					272:280	disturbed protein synthesis	254:280	disturbed protein synthesis	254:280	Those patients have disturbed protein synthesis and glycosylation in the liver.					
32594109	9	65	theme	warfarin-treated	1223:1238	arg1	individuals					1240:1250	the healthy and warfarin-treated individuals	1207:1250	the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively)	1207:1294	The main glycan was A2 in the healthy and warfarin-treated individuals (86.6 ± 4.4% and 85.6 ± 3.4%, respectively).					
32594109	11	66	from	change	1630:1635	arg1	liver					1693:1697	the liver	1689:1697	the liver	1689:1697	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	11	66	from	change	1630:1635	arg1	profile					1647:1653	glycan profile	1640:1653	glycan profile	1640:1653	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	11	66	from	change	1630:1635	arg1	function					1677:1684	protein synthetic function	1659:1684	protein synthetic function	1659:1684	CONCLUSIONS Warfarin therapy leads to lower amounts of prothrombin and Gla residues within prothrombin without exerting qualitative and quantitative change in glycan profile and protein synthetic function in the liver.					
32594109	6	67	theme	purified	674:681	arg1	PIVKA-II					706:713	the purified prothrombin containing PIVKA-II	670:713	the purified prothrombin containing PIVKA-II	670:713	Glycan structures in the purified prothrombin containing PIVKA-II were qualitatively analyzed by high performance liquid chromatography after labeling the glycan with fluorophore 2-aminobenzamide.					
32594109	3	68	theme	γ-carboxyglutamyl	343:359	arg1	residues					367:374	γ-carboxyglutamyl (Gla) residues	343:374	γ-carboxyglutamyl (Gla) residues	343:374	This decreases the number of γ-carboxyglutamyl (Gla) residues on prothrombin, converting prothrombin into PIVKA-II.					
32594109	5	69	theme	healthy	538:544	arg1	individuals					567:577	healthy and warfarin-treated individuals	538:577	healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal	538:646	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	8	70	from	production	1006:1015	arg1	liver					1024:1028	the liver	1020:1028	the liver	1020:1028	Although protein production in the liver was normal in both groups, the concentration of prothrombin was lower in the warfarin-treated individuals than in the healthy individuals (P < 0.001).					
32594109	5	71	attach	isolated	524:531	arg1	individuals					567:577	healthy and warfarin-treated individuals	538:577	healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal	538:646	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
32594109	5	71	attach	isolated	524:531	arg2	Prothrombin					508:518	METHODS Prothrombin	500:518	METHODS Prothrombin	500:518	METHODS Prothrombin was isolated from healthy and warfarin-treated individuals whose liver function of protein production was quantitatively normal.					
34174303	8	0	theme	cowpea	1246:1251	arg1	starch					1253:1258	moisture conditioned cowpea starch	1225:1258	moisture conditioned cowpea starch	1225:1258	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	8	1	theme	moisture	1225:1232	arg1	starch					1253:1258	moisture conditioned cowpea starch	1225:1258	moisture conditioned cowpea starch	1225:1258	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	10	2	theme	starch	1562:1567	arg1	properties					1548:1557	the swelling properties	1535:1557	the swelling properties of starch	1535:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	6	3	theme	pasting	791:797	arg1	temperature					799:809	pasting temperature	791:809	pasting temperature	791:809	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	8	4	theme	starch	1253:1258	arg1	crystallinity					1173:1185	crystallinity	1173:1185	crystallinity	1173:1185	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	8	4	theme	starch	1253:1258	arg1	structure					1212:1220	double-helical order structure	1191:1220	double-helical order structure	1191:1220	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	8	5	theme	double-helical	1191:1204	arg1	structure					1212:1220	double-helical order structure	1191:1220	double-helical order structure	1191:1220	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	8	6	theme	conditioned	1234:1244	arg1	starch					1253:1258	moisture conditioned cowpea starch	1225:1258	moisture conditioned cowpea starch	1225:1258	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	10	7	theme	modification	1501:1512	arg1	process					1468:1474	The infrared heating process	1447:1474	The infrared heating process	1447:1474	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	10	7	theme	modification	1501:1512	arg1	method					1514:1519	a novel and promising modification method	1479:1519	a novel and promising modification method for improving the swelling properties of starch	1479:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	7	8	theme	gelatinization	1066:1079	arg1	temperatures					1081:1092	the gelatinization temperatures	1062:1092	the gelatinization temperatures of cowpea starch	1062:1109	Infrared heating significantly reduced the gelatinization temperatures of cowpea starch but did not significantly change that of the corn starch.					
34174303	0	9	theme	starch	98:103	arg1	behaviour					78:86	the pasting and swelling behaviour	53:86	the pasting and swelling behaviour of cowpea starch	53:103	Infrared heating under optimized conditions enhanced the pasting and swelling behaviour of cowpea starch.					
34174303	6	10	theme	pasting	964:970	arg1	properties					972:981	higher functional and pasting properties	942:981	higher functional and pasting properties	942:981	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	5	11	theme	corn	719:722	arg1	starch					724:729	corn starch	719:729	corn starch	719:729	Starch samples produced under optimized conditions were compared with corn starch and their physicochemical properties determined.					
34174303	5	12	theme	Starch	649:654	arg1	samples					656:662	Starch samples	649:662	Starch samples produced under optimized conditions	649:698	Starch samples produced under optimized conditions were compared with corn starch and their physicochemical properties determined.					
34174303	2	13	theme	time	282:285	arg1	effect					226:231	the effect	222:231	the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch	222:332	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	10	14	theme	heating	1460:1466	arg1	process					1468:1474	The infrared heating process	1447:1474	The infrared heating process	1447:1474	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	10	14	theme	heating	1460:1466	arg1	method					1514:1519	a novel and promising modification method	1479:1519	a novel and promising modification method for improving the swelling properties of starch	1479:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	0	15	theme	cowpea	91:96	arg1	starch					98:103	cowpea starch	91:103	cowpea starch	91:103	Infrared heating under optimized conditions enhanced the pasting and swelling behaviour of cowpea starch.					
34174303	10	16	theme	swelling	1539:1546	arg1	properties					1548:1557	the swelling properties	1535:1557	the swelling properties of starch	1535:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	6	17	theme	native	1001:1006	arg1	starch					1015:1020	the native cowpea starch	997:1020	the native cowpea starch	997:1020	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	2	18	theme	heating	274:280	arg1	time					282:285	infrared heating time	265:285	infrared heating time	265:285	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	6	19	dep	conditions	853:862	arg1	moisture					865:872	moisture	865:872	moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min	865:935	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	3	20	theme	α	514:514	arg1	values					526:531	their corresponding α mid-point values	494:531	their corresponding α mid-point values	494:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	6	21	theme	functional	949:958	arg1	properties					972:981	higher functional and pasting properties	942:981	higher functional and pasting properties	942:981	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	3	22	dep	Factors	406:412	arg1	starch					445:450	moisture levels:10-40 g/100 g starch	415:450	moisture levels:10-40 g/100 g starch	415:450	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	22	dep	Factors	406:412	arg1	Factors					406:412	Factors	406:412	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values	406:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	22	dep	Factors	406:412	arg1	time:10-60 min					473:486	infrared heating time:10-60 min	456:486	infrared heating time:10-60 min	456:486	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	7	23	theme	Infrared	1023:1030	arg1	heating					1032:1038	Infrared heating	1023:1038	Infrared heating	1023:1038	Infrared heating significantly reduced the gelatinization temperatures of cowpea starch but did not significantly change that of the corn starch.					
34174303	2	24	theme	infrared	265:272	arg1	time					282:285	infrared heating time	265:285	infrared heating time	265:285	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	3	25	theme	infrared	456:463	arg1	Factors					406:412	Factors	406:412	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values	406:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	25	theme	infrared	456:463	arg1	time:10-60 min					473:486	infrared heating time:10-60 min	456:486	infrared heating time:10-60 min	456:486	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	4	26	theme	response	629:636	arg1	variables					638:646	response variables	629:646	response variables	629:646	Selected functional and pasting properties were determined as response variables.					
34174303	4	26	theme	response	629:636	arg1	properties					599:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties were determined as response variables.					
34174303	9	27	theme	Cowpea	1293:1298	arg1	starch					1300:1305	Cowpea starch	1293:1305	Cowpea starch	1293:1305	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	5	28	theme	optimized	679:687	arg1	conditions					689:698	optimized conditions	679:698	optimized conditions	679:698	Starch samples produced under optimized conditions were compared with corn starch and their physicochemical properties determined.					
34174303	6	29	dep	moisture	865:872	arg1	starch					889:894	46.21 g/100 g starch	875:894	46.21 g/100 g starch	875:894	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	29	dep	moisture	865:872	arg1	basis					901:905	dry basis	897:905	dry basis	897:905	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	29	dep	moisture	865:872	arg1	time					919:922	heating time	911:922	heating time	911:922	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	3	30	theme	heating	465:471	arg1	Factors					406:412	Factors	406:412	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values	406:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	30	theme	heating	465:471	arg1	time:10-60 min					473:486	infrared heating time:10-60 min	456:486	infrared heating time:10-60 min	456:486	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	31	theme	experimental	548:559	arg1	runs					561:564	13 experimental runs	545:564	13 experimental runs	545:564	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	4	32	theme	pasting	591:597	arg1	variables					638:646	response variables	629:646	response variables	629:646	Selected functional and pasting properties were determined as response variables.					
34174303	4	32	theme	pasting	591:597	arg1	properties					599:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties were determined as response variables.					
34174303	2	33	theme	rotatable	388:396	arg1	design					398:403	a two-factor central composite rotatable design	357:403	a two-factor central composite rotatable design	357:403	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	0	34	theme	Infrared	0:7	arg1	heating					9:15	Infrared heating	0:15	Infrared heating under optimized conditions	0:42	Infrared heating under optimized conditions enhanced the pasting and swelling behaviour of cowpea starch.					
34174303	3	35	with	Factors	406:412	arg1	values					526:531	their corresponding α mid-point values	494:531	their corresponding α mid-point values	494:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	9	36	theme	water	1369:1373	arg1	absorption					1375:1384	lower water absorption	1363:1384	lower water absorption	1363:1384	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	9	37	theme	bigger	1316:1321	arg1	size					1331:1334	a bigger granule size	1314:1334	a bigger granule size	1314:1334	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	9	38	theme	power	1353:1357	arg1	capacities					1386:1395	a bigger granule size, higher swelling power but lower water absorption capacities	1314:1395	a bigger granule size, higher swelling power but lower water absorption capacities	1314:1395	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	2	39	theme	composite	378:386	arg1	design					398:403	a two-factor central composite rotatable design	357:403	a two-factor central composite rotatable design	357:403	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	0	40	theme	optimized	23:31	arg1	conditions					33:42	optimized conditions	23:42	optimized conditions	23:42	Infrared heating under optimized conditions enhanced the pasting and swelling behaviour of cowpea starch.					
34174303	6	41	theme	optimal	845:851	arg1	conditions					853:862	the optimal conditions	841:862	the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min)	841:936	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	2	42	theme	physicochemical	290:304	arg1	properties					306:315	physicochemical properties	290:315	physicochemical properties of cowpea starch	290:332	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	2	43	from	effect	226:231	arg1	properties					306:315	physicochemical properties	290:315	physicochemical properties of cowpea starch	290:332	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	1	44	theme	improved	183:190	arg1	functionality					192:204	improved functionality	183:204	improved functionality	183:204	Native starches are not suitable for industrial use and must be modified for improved functionality.					
34174303	7	45	theme	corn	1156:1159	arg1	starch					1161:1166	the corn starch	1152:1166	the corn starch	1152:1166	Infrared heating significantly reduced the gelatinization temperatures of cowpea starch but did not significantly change that of the corn starch.					
34174303	9	46	theme	size	1331:1334	arg1	capacities					1386:1395	a bigger granule size, higher swelling power but lower water absorption capacities	1314:1395	a bigger granule size, higher swelling power but lower water absorption capacities	1314:1395	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	1	47	theme	Native	106:111	arg1	starches					113:120	Native starches	106:120	Native starches	106:120	Native starches are not suitable for industrial use and must be modified for improved functionality.					
34174303	3	48	theme	moisture	415:422	arg1	starch					445:450	moisture levels:10-40 g/100 g starch	415:450	moisture levels:10-40 g/100 g starch	415:450	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	48	theme	moisture	415:422	arg1	Factors					406:412	Factors	406:412	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values	406:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	6	49	theme	32.88 min	927:935	arg1	starch					889:894	46.21 g/100 g starch	875:894	46.21 g/100 g starch	875:894	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	49	theme	32.88 min	927:935	arg1	basis					901:905	dry basis	897:905	dry basis	897:905	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	49	theme	32.88 min	927:935	arg1	time					919:922	heating time	911:922	heating time	911:922	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	3	50	theme	levels:10-40 g/100 g	424:443	arg1	starch					445:450	moisture levels:10-40 g/100 g starch	415:450	moisture levels:10-40 g/100 g starch	415:450	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	3	50	theme	levels:10-40 g/100 g	424:443	arg1	Factors					406:412	Factors	406:412	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values	406:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	9	51	theme	higher	1337:1342	arg1	power					1353:1357	higher swelling power	1337:1357	higher swelling power	1337:1357	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	7	52	theme	starch	1104:1109	arg1	temperatures					1081:1092	the gelatinization temperatures	1062:1092	the gelatinization temperatures of cowpea starch	1062:1109	Infrared heating significantly reduced the gelatinization temperatures of cowpea starch but did not significantly change that of the corn starch.					
34174303	0	53	theme	pasting	57:63	arg1	behaviour					78:86	the pasting and swelling behaviour	53:86	the pasting and swelling behaviour of cowpea starch	53:103	Infrared heating under optimized conditions enhanced the pasting and swelling behaviour of cowpea starch.					
34174303	6	54	theme	cowpea	1008:1013	arg1	starch					1015:1020	the native cowpea starch	997:1020	the native cowpea starch	997:1020	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	55	theme	46.21 g/100 g	875:887	arg1	starch					889:894	46.21 g/100 g starch	875:894	46.21 g/100 g starch	875:894	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	9	56	theme	swelling	1344:1351	arg1	power					1353:1357	higher swelling power	1337:1357	higher swelling power	1337:1357	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	9	57	theme	granule	1323:1329	arg1	size					1331:1334	a bigger granule size	1314:1334	a bigger granule size	1314:1334	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	3	58	theme	mid-point	516:524	arg1	values					526:531	their corresponding α mid-point values	494:531	their corresponding α mid-point values	494:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	9	59	theme	pasting	1401:1407	arg1	properties					1409:1418	pasting properties	1401:1418	pasting properties	1401:1418	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	4	60	theme	functional	576:585	arg1	variables					638:646	response variables	629:646	response variables	629:646	Selected functional and pasting properties were determined as response variables.					
34174303	4	60	theme	functional	576:585	arg1	properties					599:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties were determined as response variables.					
34174303	8	61	dep	crystallinity	1173:1185	arg1	The					1169:1171	The	1169:1171	The	1169:1171	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	6	62	theme	heating	911:917	arg1	time					919:922	heating time	911:922	heating time	911:922	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	1	63	theme	industrial	143:152	arg1	use					154:156	industrial use	143:156	industrial use	143:156	Native starches are not suitable for industrial use and must be modified for improved functionality.					
34174303	4	64	theme	Selected	567:574	arg1	variables					638:646	response variables	629:646	response variables	629:646	Selected functional and pasting properties were determined as response variables.					
34174303	4	64	theme	Selected	567:574	arg1	properties					599:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties	567:608	Selected functional and pasting properties were determined as response variables.					
34174303	5	65	theme	physicochemical	741:755	arg1	properties					757:766	their physicochemical properties	735:766	their physicochemical properties determined	735:777	Starch samples produced under optimized conditions were compared with corn starch and their physicochemical properties determined.					
34174303	2	66	theme	preconditioning	245:259	arg1	effect					226:231	the effect	222:231	the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch	222:332	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	7	67	theme	cowpea	1097:1102	arg1	starch					1104:1109	cowpea starch	1097:1109	cowpea starch	1097:1109	Infrared heating significantly reduced the gelatinization temperatures of cowpea starch but did not significantly change that of the corn starch.					
34174303	2	68	theme	starch	327:332	arg1	properties					306:315	physicochemical properties	290:315	physicochemical properties of cowpea starch	290:332	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	8	69	theme	order	1206:1210	arg1	structure					1212:1220	double-helical order structure	1191:1220	double-helical order structure	1191:1220	The crystallinity and double-helical order structure of moisture conditioned cowpea starch also reduced after modification.					
34174303	9	70	theme	lower	1363:1367	arg1	absorption					1375:1384	lower water absorption	1363:1384	lower water absorption	1363:1384	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	2	71	theme	moisture	236:243	arg1	preconditioning					245:259	moisture preconditioning	236:259	moisture preconditioning	236:259	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	6	72	theme	cowpea	812:817	arg1	starch					819:824	cowpea starch	812:824	cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min)	812:936	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	2	73	theme	cowpea	320:325	arg1	starch					327:332	cowpea starch	320:332	cowpea starch	320:332	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	10	74	theme	infrared	1451:1458	arg1	process					1468:1474	The infrared heating process	1447:1474	The infrared heating process	1447:1474	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	10	74	theme	infrared	1451:1458	arg1	method					1514:1519	a novel and promising modification method	1479:1519	a novel and promising modification method for improving the swelling properties of starch	1479:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	3	75	theme	corresponding	500:512	arg1	values					526:531	their corresponding α mid-point values	494:531	their corresponding α mid-point values	494:531	Factors (moisture levels:10-40 g/100 g starch and infrared heating time:10-60 min) with their corresponding α mid-point values resulted in 13 experimental runs.					
34174303	0	76	theme	swelling	69:76	arg1	behaviour					78:86	the pasting and swelling behaviour	53:86	the pasting and swelling behaviour of cowpea starch	53:103	Infrared heating under optimized conditions enhanced the pasting and swelling behaviour of cowpea starch.					
34174303	6	77	theme	dry	897:899	arg1	basis					901:905	dry basis	897:905	dry basis	897:905	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	78	contain	had	938:940	arg2	properties					972:981	higher functional and pasting properties	942:981	higher functional and pasting properties	942:981	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	6	78	contain	had	938:940	arg1	starch					819:824	cowpea starch	812:824	cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min)	812:936	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
34174303	2	79	theme	central	370:376	arg1	design					398:403	a two-factor central composite rotatable design	357:403	a two-factor central composite rotatable design	357:403	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	10	80	theme	novel	1481:1485	arg1	process					1468:1474	The infrared heating process	1447:1474	The infrared heating process	1447:1474	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	10	80	theme	novel	1481:1485	arg1	method					1514:1519	a novel and promising modification method	1479:1519	a novel and promising modification method for improving the swelling properties of starch	1479:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	9	81	theme	absorption	1375:1384	arg1	capacities					1386:1395	a bigger granule size, higher swelling power but lower water absorption capacities	1314:1395	a bigger granule size, higher swelling power but lower water absorption capacities	1314:1395	Cowpea starch showed a bigger granule size, higher swelling power but lower water absorption capacities and pasting properties compared with the control.					
34174303	10	82	theme	promising	1491:1499	arg1	process					1468:1474	The infrared heating process	1447:1474	The infrared heating process	1447:1474	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	10	82	theme	promising	1491:1499	arg1	method					1514:1519	a novel and promising modification method	1479:1519	a novel and promising modification method for improving the swelling properties of starch	1479:1567	The infrared heating process is a novel and promising modification method for improving the swelling properties of starch.					
34174303	2	83	theme	two-factor	359:368	arg1	design					398:403	a two-factor central composite rotatable design	357:403	a two-factor central composite rotatable design	357:403	In this study, the effect of moisture preconditioning and infrared heating time on physicochemical properties of cowpea starch was investigated using a two-factor central composite rotatable design.					
34174303	6	84	dep	functional	949:958	arg1	higher					942:947	higher	942:947	higher	942:947	Except for pasting temperature, cowpea starch prepared using the optimal conditions (moisture: 46.21 g/100 g starch, dry basis and heating time of 32.88 min) had higher functional and pasting properties compared with the native cowpea starch.					
31981775	8	0	dep	cytokines	2147:2155	arg1	IL-10					2158:2162	IL-10	2158:2162	IL-10	2158:2162	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	8	0	dep	cytokines	2147:2155	arg1	TGF-β					2165:2169	TGF-β	2165:2169	TGF-β	2165:2169	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	0	1	theme	Microbial	183:191	arg1	EPS					213:215	EPS	213:215	EPS	213:215	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	1	theme	Microbial	183:191	arg1	exopolysaccharides					193:210	Cyprinus carpio L. Microbial exopolysaccharides	164:210	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	1	2	theme	present	431:437	arg1	study					439:443	the present study	427:443	the present study	427:443	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	5	3	theme	head	1249:1252	arg1	cells					1261:1265	head kidney cells	1249:1265	head kidney cells	1249:1265	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	9	4	theme	immunomodulatory	2281:2296	arg1	effects					2314:2320	immunomodulatory and antioxidant effects	2281:2320	immunomodulatory and antioxidant effects	2281:2320	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	5	5	dep	cytokines	1156:1164	arg1	TNF-α					1167:1171	TNF-α	1167:1171	TNF-α	1167:1171	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	5	5	dep	cytokines	1156:1164	arg1	IL-6					1181:1184	IL-6	1181:1184	IL-6	1181:1184	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	5	5	dep	cytokines	1156:1164	arg1	IL-1β					1174:1178	IL-1β	1174:1178	IL-1β	1174:1178	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	7	6	theme	pro-inflammatory	1734:1749	arg1	cytokines					1751:1759	pro-inflammatory cytokines	1734:1759	pro-inflammatory cytokines (TNF-α, IL-1β, IL-6)	1734:1780	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	6	theme	pro-inflammatory	1734:1749	arg1	TNF-α					1762:1766	TNF-α	1762:1766	TNF-α	1762:1766	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	1	7	from	effects	573:579	arg1	Cyprinus					636:643	Cyprinus	636:643	Cyprinus	636:643	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	7	8	with	infection	1618:1626	arg1	hydrophila					1636:1645	A. hydrophila	1633:1645	A. hydrophila	1633:1645	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	9	contain	have	254:257	arg1	resistance					121:130	disease resistance	113:130	disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	113:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	9	contain	have	254:257	arg2	role					272:275	an important role	259:275	an important role	259:275	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	9	contain	have	254:257	arg1	Effects					0:6	Effects	0:6	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response	0:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	9	contain	have	254:257	arg1	activity					99:106	antioxidant activity	87:106	antioxidant activity	87:106	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	3	10	theme	monosaccharide	718:731	arg1	rhamnose					765:772	rhamnose	765:772	rhamnose	765:772	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	3	10	theme	monosaccharide	718:731	arg1	composition					733:743	The monosaccharide composition	714:743	The monosaccharide composition of this polymer	714:759	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	5	11	theme	In	967:968	arg1	tests					976:980	In vitro tests	967:980	In vitro tests	967:980	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	6	12	theme	alkaline	1479:1486	arg1	AKP					1501:1503	AKP	1501:1503	AKP	1501:1503	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	12	theme	alkaline	1479:1486	arg1	phosphatase					1488:1498	alkaline phosphatase	1479:1498	alkaline phosphatase (AKP)	1479:1504	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	13	theme	different	1407:1415	arg1	patterns					1428:1435	different expression patterns	1407:1435	different expression patterns of the NO	1407:1445	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	7	14	theme	antioxidant	1821:1831	arg1	molecules					1833:1841	antioxidant molecules	1821:1841	antioxidant molecules	1821:1841	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	3	15	theme	polymer	753:759	arg1	rhamnose					765:772	rhamnose	765:772	rhamnose	765:772	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	3	15	theme	polymer	753:759	arg1	composition					733:743	The monosaccharide composition	714:743	The monosaccharide composition of this polymer	714:759	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	0	16	from	activity	99:106	arg1	EPS					213:215	EPS	213:215	EPS	213:215	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	16	from	activity	99:106	arg1	Lactococcus					223:233	Lactococcus	223:233	Lactococcus	223:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	16	from	activity	99:106	arg1	exopolysaccharides					193:210	Cyprinus carpio L. Microbial exopolysaccharides	164:210	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	16	from	activity	99:106	arg1	response					77:84	innate immune response	63:84	innate immune response	63:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	16	from	activity	99:106	arg1	Z-2					56:58	Lactococcus lactis Z-2	37:58	Lactococcus lactis Z-2	37:58	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	17	theme	probiotic	284:292	arg1	activity					294:301	the probiotic activity	280:301	the probiotic activity of this bacterium	280:319	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	3	18	theme	molar	820:824	arg1	percentage					826:835	a molar percentage	818:835	a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively	818:890	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	7	19	theme	G2	2032:2033	arg1	group					2036:2040	the positive (G2) group	2018:2040	the positive (G2) group	2018:2040	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	2	20	theme	molecular	673:681	arg1	18.65 KDa					703:711	18.65 KDa	703:711	18.65 KDa	703:711	We found that the molecular weight of EPS-2 was 18.65 KDa.					
31981775	2	20	theme	molecular	673:681	arg1	weight					683:688	the molecular weight	669:688	the molecular weight of EPS-2	669:697	We found that the molecular weight of EPS-2 was 18.65 KDa.					
31981775	8	21	theme	cytokines	2147:2155	arg1	levels					2119:2124	the expression levels	2104:2124	the expression levels of anti-inflammatory cytokines (IL-10, TGF-β)	2104:2170	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	8	22	theme	treatment	2214:2222	arg1	groups					2224:2229	the EPS-2 treatment groups	2204:2229	the EPS-2 treatment groups (P < 0.05)	2204:2240	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	8	22	theme	treatment	2214:2222	arg1	P < 0.05					2232:2239	P < 0.05	2232:2239	P < 0.05	2232:2239	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	7	23	theme	positive	2022:2029	arg1	group					2036:2040	the positive (G2) group	2018:2040	the positive (G2) group	2018:2040	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	24	theme	bacterium	311:319	arg1	activity					294:301	the probiotic activity	280:301	the probiotic activity of this bacterium	280:319	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	3	25	theme	%	844:844	arg1	percentage					826:835	a molar percentage	818:835	a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively	818:890	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	4	26	theme	EPS-2	893:897	arg1	treatment					899:907	EPS-2 treatment	893:907	EPS-2 treatment	893:907	EPS-2 treatment could modulate the immune responses in vitro and in vivo.					
31981775	7	27	theme	AKP	1791:1793	arg1	activities					1795:1804	AKP activities	1791:1804	AKP activities	1791:1804	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	28	theme	immune	70:75	arg1	response					77:84	innate immune response	63:84	innate immune response	63:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	8	29	theme	expression	2108:2117	arg1	levels					2119:2124	the expression levels	2104:2124	the expression levels of anti-inflammatory cytokines (IL-10, TGF-β)	2104:2170	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	1	30	attach	isolated	497:504	arg2	EPS-2					462:466	EPS-2	462:466	EPS-2	462:466	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	1	30	attach	isolated	497:504	arg1	carp					526:529	healthy common carp	511:529	healthy common carp	511:529	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	0	31	from	Effects	0:6	arg1	EPS					213:215	EPS	213:215	EPS	213:215	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	31	from	Effects	0:6	arg1	Lactococcus					223:233	Lactococcus	223:233	Lactococcus	223:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	31	from	Effects	0:6	arg1	exopolysaccharides					193:210	Cyprinus carpio L. Microbial exopolysaccharides	164:210	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	31	from	Effects	0:6	arg1	response					77:84	innate immune response	63:84	innate immune response	63:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	31	from	Effects	0:6	arg1	Z-2					56:58	Lactococcus lactis Z-2	37:58	Lactococcus lactis Z-2	37:58	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	7	32	theme	LZM	1956:1958	arg1	activities					1968:1977	LZM and AKP activities	1956:1977	activities	1968:1977	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	5	33	theme	cytokines	1156:1164	arg1	production					1107:1116	the production	1103:1116	the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells	1103:1265	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	9	34	theme	carp	2399:2402	arg1	supplement					2409:2418	a common carp feed supplement	2390:2418	a common carp feed supplement	2390:2418	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	9	34	theme	carp	2399:2402	arg1	EPS-2					2271:2275	EPS-2	2271:2275	EPS-2	2271:2275	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	1	35	dep	lactis	485:490	arg1	Z-2					492:494	Z-2	492:494	Lactococcus lactis Z-2	473:494	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	7	36	theme	cytokines	1942:1950	arg1	activities					1968:1977	LZM and AKP activities	1956:1977	activities	1968:1977	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	36	theme	cytokines	1942:1950	arg1	levels					1908:1913	levels	1908:1913	levels of NO and pro-inflammatory cytokines	1908:1950	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	5	37	theme	anti-inflammatory	1192:1208	arg1	P < 0.05					1236:1243	P < 0.05	1236:1243	P < 0.05	1236:1243	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	5	37	theme	anti-inflammatory	1192:1208	arg1	cytokines					1210:1218	anti-inflammatory cytokines	1192:1218	anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05)	1192:1244	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	9	38	contain	has	2277:2279	arg2	effects					2314:2320	immunomodulatory and antioxidant effects	2281:2320	immunomodulatory and antioxidant effects	2281:2320	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	9	38	contain	has	2277:2279	arg1	supplement					2409:2418	a common carp feed supplement	2390:2418	a common carp feed supplement	2390:2418	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	9	38	contain	has	2277:2279	arg1	EPS-2					2271:2275	EPS-2	2271:2275	EPS-2	2271:2275	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	7	39	theme	EPS-2	1648:1652	arg1	supplementation					1654:1668	EPS-2 supplementation	1648:1668	EPS-2 supplementation	1648:1668	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	40	theme	important	262:270	arg1	role					272:275	an important role	259:275	an important role	259:275	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	1	41	theme	immunomodulatory	540:555	arg1	effects					573:579	its immunomodulatory and antioxidant effects	536:579	its immunomodulatory and antioxidant effects	536:579	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	3	42	theme	%	858:858	arg1	percentage					826:835	a molar percentage	818:835	a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively	818:890	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	8	43	dep	whether	2079:2085	arg1	not					2099:2101	not	2099:2101	not	2099:2101	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	8	43	dep	whether	2079:2085	arg1	infected					2087:2094	infected	2087:2094	infected	2087:2094	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	1	44	theme	antioxidant	561:571	arg1	effects					573:579	its immunomodulatory and antioxidant effects	536:579	its immunomodulatory and antioxidant effects	536:579	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	5	45	theme	phagocytosis	1050:1061	arg1	activities					1063:1072	phagocytosis activities	1050:1072	phagocytosis activities	1050:1072	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	5	46	dep	cytokines	1210:1218	arg1	TGF-β					1228:1232	TGF-β	1228:1232	TGF-β	1228:1232	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	5	46	dep	cytokines	1210:1218	arg1	IL-10					1221:1225	IL-10	1221:1225	IL-10	1221:1225	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	1	47	theme	Lactococcus	473:483	arg1	lactis					485:490	Lactococcus lactis Z-2	473:494	Lactococcus lactis Z-2	473:494	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	0	48	theme	lactis	49:54	arg1	Z-2					56:58	Lactococcus lactis Z-2	37:58	Lactococcus lactis Z-2	37:58	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	6	49	theme	antioxidants	1526:1537	arg1	concentrations					1316:1329	three different concentrations	1300:1329	three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA)	1300:1576	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	50	theme	EPS-2	1334:1338	arg1	concentrations					1316:1329	three different concentrations	1300:1329	three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA)	1300:1576	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	5	51	theme	nitic	1121:1125	arg1	oxide					1127:1131	nitic oxide	1121:1131	nitic oxide (NO)	1121:1136	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	5	51	theme	nitic	1121:1125	arg1	NO					1134:1135	NO	1134:1135	NO	1134:1135	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	0	52	from	resistance	121:130	arg1	EPS					213:215	EPS	213:215	EPS	213:215	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	52	from	resistance	121:130	arg1	Lactococcus					223:233	Lactococcus	223:233	Lactococcus	223:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	52	from	resistance	121:130	arg1	exopolysaccharides					193:210	Cyprinus carpio L. Microbial exopolysaccharides	164:210	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	52	from	resistance	121:130	arg1	response					77:84	innate immune response	63:84	innate immune response	63:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	52	from	resistance	121:130	arg1	Z-2					56:58	Lactococcus lactis Z-2	37:58	Lactococcus lactis Z-2	37:58	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	9	53	theme	disease	2449:2455	arg1	resistance					2457:2466	disease resistance	2449:2466	disease resistance	2449:2466	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	1	54	theme	healthy	511:517	arg1	carp					526:529	healthy common carp	511:529	healthy common carp	511:529	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	7	55	dep	TNF-α	1762:1766	arg1	IL-6					1776:1779	IL-6	1776:1779	IL-6	1776:1779	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	55	dep	TNF-α	1762:1766	arg1	IL-1β					1769:1773	IL-1β	1769:1773	IL-1β	1769:1773	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	5	56	theme	kidney	1254:1259	arg1	cells					1261:1265	head kidney cells	1249:1265	head kidney cells	1249:1265	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	1	57	from	resistance	593:602	arg1	Cyprinus					636:643	Cyprinus	636:643	Cyprinus	636:643	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	7	58	theme	negative	1868:1875	arg1	P < 0.05					1889:1896	P < 0.05	1889:1896	P < 0.05	1889:1896	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	58	theme	negative	1868:1875	arg1	group					1882:1886	the negative (G1) group	1864:1886	the negative (G1) group (P < 0.05)	1864:1897	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	58	theme	negative	1868:1875	arg1	G1					1878:1879	G1	1878:1879	G1	1878:1879	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	59	theme	disease	113:119	arg1	resistance					121:130	disease resistance	113:130	disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	113:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	60	theme	immunomodulatory	335:350	arg1	activities					368:377	the immunomodulatory and antioxidant activities	331:377	the immunomodulatory and antioxidant activities	331:377	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	7	61	theme	cytokines	1751:1759	arg1	levels					1724:1729	protein levels	1716:1729	protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities	1716:1804	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	61	theme	cytokines	1751:1759	arg1	levels					1811:1816	levels	1811:1816	levels of antioxidant molecules	1811:1841	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	61	theme	cytokines	1751:1759	arg1	production					1704:1713	the NO production	1697:1713	the NO production	1697:1713	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	3	62	from	percentage	826:835	arg1	mannose					783:789	mannose	783:789	mannose	783:789	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	3	62	from	percentage	826:835	arg1	rhamnose					765:772	rhamnose	765:772	rhamnose	765:772	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	3	62	from	percentage	826:835	arg1	glucose					792:798	glucose	792:798	glucose	792:798	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	3	62	from	percentage	826:835	arg1	galactose					805:813	galactose	805:813	galactose	805:813	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	9	63	theme	common	2392:2397	arg1	carp					2399:2402	a common carp	2390:2402	a common carp feed supplement	2390:2418	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	0	64	theme	antioxidant	356:366	arg1	activities					368:377	the immunomodulatory and antioxidant activities	331:377	the immunomodulatory and antioxidant activities	331:377	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	9	65	theme	common	2325:2330	arg1	carp					2332:2335	common carp	2325:2335	common carp	2325:2335	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	9	66	theme	antioxidant	2302:2312	arg1	effects					2314:2320	immunomodulatory and antioxidant effects	2281:2320	immunomodulatory and antioxidant effects	2281:2320	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	2	67	theme	EPS-2	693:697	arg1	18.65 KDa					703:711	18.65 KDa	703:711	18.65 KDa	703:711	We found that the molecular weight of EPS-2 was 18.65 KDa.					
31981775	2	67	theme	EPS-2	693:697	arg1	weight					683:688	the molecular weight	669:688	the molecular weight of EPS-2	669:697	We found that the molecular weight of EPS-2 was 18.65 KDa.					
31981775	0	68	dep	Cyprinus	164:171	arg1	L.					180:181	Cyprinus carpio L.	164:181	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	7	69	theme	protein	1716:1722	arg1	levels					1724:1729	protein levels	1716:1729	protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities	1716:1804	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	5	70	from	production	1107:1116	arg1	cells					1261:1265	head kidney cells	1249:1265	head kidney cells	1249:1265	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	7	71	theme	molecules	1833:1841	arg1	levels					1724:1729	protein levels	1716:1729	protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities	1716:1804	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	71	theme	molecules	1833:1841	arg1	levels					1811:1816	levels	1811:1816	levels of antioxidant molecules	1811:1841	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	71	theme	molecules	1833:1841	arg1	production					1704:1713	the NO production	1697:1713	the NO production	1697:1713	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	8	72	theme	EPS-2	2208:2212	arg1	groups					2224:2229	the EPS-2 treatment groups	2204:2229	the EPS-2 treatment groups (P < 0.05)	2204:2240	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	8	72	theme	EPS-2	2208:2212	arg1	P < 0.05					2232:2239	P < 0.05	2232:2239	P < 0.05	2232:2239	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	0	73	theme	Cyprinus	164:171	arg1	EPS					213:215	EPS	213:215	EPS	213:215	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	73	theme	Cyprinus	164:171	arg1	exopolysaccharides					193:210	Cyprinus carpio L. Microbial exopolysaccharides	164:210	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	6	74	dep	days	1369:1372	arg1	infected					1378:1385	infected	1378:1385	infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum	1378:1517	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	0	75	from	Lactococcus	223:233	arg1	resistance					121:130	disease resistance	113:130	disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	113:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	75	from	Lactococcus	223:233	arg1	EPS					213:215	EPS	213:215	EPS	213:215	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	75	from	Lactococcus	223:233	arg1	Effects					0:6	Effects	0:6	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response	0:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	75	from	Lactococcus	223:233	arg1	exopolysaccharides					193:210	Cyprinus carpio L. Microbial exopolysaccharides	164:210	Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	164:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	75	from	Lactococcus	223:233	arg1	activity					99:106	antioxidant activity	87:106	antioxidant activity	87:106	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	1	76	theme	disease	585:591	arg1	resistance					593:602	disease resistance	585:602	disease resistance against Aeromonas hydrophila in Cyprinus carpio L	585:652	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	0	77	theme	innate	63:68	arg1	response					77:84	innate immune response	63:84	innate immune response	63:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	7	78	theme	NO	1701:1702	arg1	production					1704:1713	the NO production	1697:1713	the NO production	1697:1713	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	79	theme	activities	1795:1804	arg1	levels					1724:1729	protein levels	1716:1729	protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities	1716:1804	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	79	theme	activities	1795:1804	arg1	levels					1811:1816	levels	1811:1816	levels of antioxidant molecules	1811:1841	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	79	theme	activities	1795:1804	arg1	production					1704:1713	the NO production	1697:1713	the NO production	1697:1713	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	5	80	theme	pro-inflammatory	1139:1154	arg1	cytokines					1156:1164	pro-inflammatory cytokines	1139:1164	pro-inflammatory cytokines (TNF-α, IL-1β, IL-6)	1139:1185	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	0	81	theme	antioxidant	87:97	arg1	activity					99:106	antioxidant activity	87:106	antioxidant activity	87:106	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	1	82	dep	Cyprinus	636:643	arg1	L					652:652	L	652:652	Cyprinus carpio L	636:652	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	1	82	dep	Cyprinus	636:643	arg1	carpio					645:650	Cyprinus carpio L	636:652	Cyprinus carpio L	636:652	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	8	83	theme	anti-inflammatory	2129:2145	arg1	cytokines					2147:2155	anti-inflammatory cytokines	2129:2155	anti-inflammatory cytokines (IL-10, TGF-β)	2129:2170	However, whether infected or not, the expression levels of anti-inflammatory cytokines (IL-10, TGF-β) were significantly increased in the EPS-2 treatment groups (P < 0.05).					
31981775	0	84	from	Z-2	56:58	arg1	resistance					121:130	disease resistance	113:130	disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	113:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	84	from	Z-2	56:58	arg1	Effects					0:6	Effects	0:6	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response	0:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	84	from	Z-2	56:58	arg1	exopolysaccharide					14:30	an exopolysaccharide	11:30	an exopolysaccharide from Lactococcus lactis Z-2	11:58	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	84	from	Z-2	56:58	arg1	activity					99:106	antioxidant activity	87:106	antioxidant activity	87:106	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	1	85	theme	common	519:524	arg1	carp					526:529	healthy common carp	511:529	healthy common carp	511:529	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	6	86	theme	NO	1444:1445	arg1	patterns					1428:1435	different expression patterns	1407:1435	different expression patterns of the NO	1407:1445	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	7	87	theme	AKP	1964:1966	arg1	activities					1968:1977	LZM and AKP activities	1956:1977	activities	1968:1977	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	5	88	theme	cytokines	1210:1218	arg1	production					1107:1116	the production	1103:1116	the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells	1103:1265	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	3	89	theme	%	851:851	arg1	percentage					826:835	a molar percentage	818:835	a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively	818:890	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	4	90	theme	immune	928:933	arg1	responses					935:943	the immune responses	924:943	the immune responses	924:943	EPS-2 treatment could modulate the immune responses in vitro and in vivo.					
31981775	5	91	dep	proliferation	1032:1044	arg1	the					1028:1030	the	1028:1030	the	1028:1030	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	7	92	theme	pro-inflammatory	1925:1940	arg1	cytokines					1942:1950	pro-inflammatory cytokines	1925:1950	pro-inflammatory cytokines	1925:1950	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	93	theme	exopolysaccharide	14:30	arg1	resistance					121:130	disease resistance	113:130	disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus	113:233	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	93	theme	exopolysaccharide	14:30	arg1	Effects					0:6	Effects	0:6	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response	0:84	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	0	93	theme	exopolysaccharide	14:30	arg1	activity					99:106	antioxidant activity	87:106	antioxidant activity	87:106	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	6	94	theme	expression	1417:1426	arg1	patterns					1428:1435	different expression patterns	1407:1435	different expression patterns of the NO	1407:1445	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	3	95	theme	%	865:865	arg1	percentage					826:835	a molar percentage	818:835	a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively	818:890	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	1	96	from	lactis	485:490	arg1	EPS-2					462:466	EPS-2	462:466	EPS-2	462:466	In the present study, we investigated EPS-2 from Lactococcus lactis Z-2, isolated from healthy common carp, for its immunomodulatory and antioxidant effects and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.					
31981775	7	97	theme	NO	1918:1919	arg1	activities					1968:1977	LZM and AKP activities	1956:1977	activities	1968:1977	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	97	theme	NO	1918:1919	arg1	levels					1908:1913	levels	1908:1913	levels of NO and pro-inflammatory cytokines	1908:1950	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	0	98	theme	Lactococcus	37:47	arg1	lactis					49:54	Lactococcus lactis	37:54	Lactococcus lactis Z-2	37:58	Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L. Microbial exopolysaccharides (EPS) from Lactococcus have been found to have an important role in the probiotic activity of this bacterium; however, the immunomodulatory and antioxidant activities have not been fully explored in aquaculture.					
31981775	6	99	dep	antioxidants	1526:1537	arg1	T-AOC					1540:1544	T-AOC	1540:1544	T-AOC	1540:1544	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	9	100	theme	feed	2404:2407	arg1	supplement					2409:2418	a common carp feed supplement	2390:2418	a common carp feed supplement	2390:2418	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	9	100	theme	feed	2404:2407	arg1	EPS-2					2271:2275	EPS-2	2271:2275	EPS-2	2271:2275	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	6	101	dep	T-AOC	1540:1544	arg1	GSH-Px					1562:1567	GSH-Px	1562:1567	GSH-Px	1562:1567	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	101	dep	T-AOC	1540:1544	arg1	GSH					1557:1559	GSH	1557:1559	GSH	1557:1559	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	101	dep	T-AOC	1540:1544	arg1	CAT					1552:1554	CAT	1552:1554	CAT	1552:1554	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	101	dep	T-AOC	1540:1544	arg1	SOD					1547:1549	SOD	1547:1549	SOD	1547:1549	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	6	101	dep	T-AOC	1540:1544	arg1	MDA					1573:1575	MDA	1573:1575	MDA	1573:1575	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	3	102	theme	%	876:876	arg1	percentage					826:835	a molar percentage	818:835	a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively	818:890	The monosaccharide composition of this polymer was rhamnose, xylose, mannose, glucose, and galactose at a molar percentage of 13.3%, 14.1%, 18.5%, 27.4%, and 26.7%, respectively.					
31981775	9	103	theme	fish	2431:2434	arg1	immunity					2436:2443	fish immunity	2431:2443	fish immunity	2431:2443	These results indicate that EPS-2 has immunomodulatory and antioxidant effects on common carp, both in vitro and/or in vivo, and can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.					
31981775	5	104	theme	oxide	1127:1131	arg1	production					1107:1116	the production	1103:1116	the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells	1103:1265	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	7	105	theme	LZM	1783:1785	arg1	levels					1724:1729	protein levels	1716:1729	protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities	1716:1804	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	105	theme	LZM	1783:1785	arg1	levels					1811:1816	levels	1811:1816	levels of antioxidant molecules	1811:1841	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	7	105	theme	LZM	1783:1785	arg1	production					1704:1713	the NO production	1697:1713	the NO production	1697:1713	Before infection with A. hydrophila, EPS-2 supplementation significantly up-regulated the NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), LZM and AKP activities, and levels of antioxidant molecules compared to those in the negative (G1) group (P < 0.05), whereas levels of NO and pro-inflammatory cytokines and LZM and AKP activities were significantly lower than those in the positive (G2) group after infection (P < 0.05).					
31981775	6	106	dep	concentrations	1316:1329	arg1	1000 μg/mL					1351:1360	1000 μg/mL	1351:1360	1000 μg/mL	1351:1360	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
31981775	5	107	dep	In	967:968	arg1	vitro					970:974	vitro	970:974	vitro	970:974	In vitro tests showed that EPS-2 could significantly enhance the proliferation and phagocytosis activities (P < 0.05) as well as induce the production of nitic oxide (NO), pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in head kidney cells.					
31981775	6	108	theme	different	1306:1314	arg1	concentrations					1316:1329	three different concentrations	1300:1329	three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA)	1300:1576	When the fish were gavaged with three different concentrations of EPS-2 (250, 500, 1000 μg/mL) for 7 days and infected with A. hydrophila, different expression patterns of the NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in the serum and of antioxidants (T-AOC, SOD, CAT, GSH, GSH-Px and MDA) in hepatopancreas were observed.					
34871808	3	0	from	ways	727:730	arg1	analysis					693:700	proteomic analysis	683:700	proteomic analysis of organoids in multiple ways	683:730	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	6	1	theme	yield	1333:1337	arg1	labels					1433:1438	the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels	1313:1438	the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%)	1313:1446	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	1	theme	yield	1333:1337	arg1	%					1445:1445	97.1%	1441:1445	97.1%	1441:1445	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	2	theme	least	1469:1473	arg1	contaminants					1494:1505	the least potential Matrigel contaminants	1465:1505	the least potential Matrigel contaminants	1465:1505	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	8	3	dep	identifies	1781:1790	arg1	valuable					1913:1920	valuable	1913:1920	valuable	1913:1920	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	3	4	from	analysis	693:700	arg1	ways					727:730	multiple ways	718:730	multiple ways	718:730	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	6	5	theme	highest	1317:1323	arg1	yield					1333:1337	the highest peptide yield	1313:1337	the highest peptide yield	1313:1337	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	3	6	theme	identical	575:583	arg1	peptides					585:592	identical peptides	575:592	identical peptides shared between Matrigel and organoids	575:630	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	3	7	from	organoids	705:713	arg1	ways					727:730	multiple ways	718:730	multiple ways	718:730	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	8	8	theme	translational	1936:1948	arg1	research					1960:1967	basic and translational proteomic research	1926:1967	research	1960:1967	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	1	9	theme	molecular	202:210	arg1	characteristics					212:226	histological and molecular characteristics	185:226	histological and molecular characteristics of original tissues	185:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	6	10	theme	organoids	1204:1212	arg1	proteomes					1191:1199	cell culture proteomes	1178:1199	cell culture proteomes of organoids collected by three methods, respectively	1178:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	11	theme	isotope	1385:1391	arg1	labeling					1393:1400	stable isotope labeling	1378:1400	stable isotope labeling by amino acids in cell culture	1378:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	5	12	theme	Matrigel	892:899	arg1	buffer					967:972	PBS-EDTA buffer	958:972	PBS-EDTA buffer	958:972	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	12	theme	Matrigel	892:899	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	12	theme	Matrigel	892:899	arg1	dispase					945:951	dispase	945:951	dispase	945:951	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	12	theme	Matrigel	892:899	arg1	solution					935:942	cell recovery solution	921:942	cell recovery solution	921:942	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	13	theme	optimal	1288:1294	arg1	efficiency					1296:1305	an optimal efficiency	1285:1305	an optimal efficiency	1285:1305	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	4	14	theme	Matrigel	755:762	arg1	matrix					764:769	Matrigel matrix	755:769	Matrigel matrix	755:769	Thus, how to dissolve Matrigel matrix and recovery organoid cells efficiently is vital for sample preparation.					
34871808	6	15	theme	incorporation	1355:1367	arg1	ratio					1369:1373	the highest incorporation ratio	1343:1373	the highest incorporation ratio of stable isotope labeling by amino acids in cell culture	1343:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	8	16	theme	experimental	1811:1822	arg1	approaches					1824:1833	experimental approaches	1811:1833	experimental approaches	1811:1833	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	1	17	theme	promising	134:142	arg1	models					163:168	promising ex vivo 3D culture models	134:168	promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues	134:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	1	17	theme	promising	134:142	arg1	profiling					264:272	proteomic profiling	254:272	proteomic profiling of organoids	254:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	7	18	theme	Matrigel	1630:1637	arg1	contaminants					1639:1650	312 high-confidence Matrigel contaminants	1610:1650	312 high-confidence Matrigel contaminants	1610:1650	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	1	19	theme	3D	152:153	arg1	models					163:168	promising ex vivo 3D culture models	134:168	promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues	134:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	1	19	theme	3D	152:153	arg1	profiling					264:272	proteomic profiling	254:272	proteomic profiling of organoids	254:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	6	20	theme	integrative	1075:1085	arg1	analysis					1087:1094	integrative analysis	1075:1094	integrative analysis of label-free proteomes of Matrigel	1075:1130	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	7	21	theme	information	1748:1758	arg1	loss					1729:1732	minimal loss	1721:1732	minimal loss of biological information	1721:1758	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	0	22	theme	Organoids	77:85	arg1	Profiling					64:72	Proteomic Profiling	54:72	Proteomic Profiling of Organoids	54:85	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	8	23	theme	Matrigel	1864:1871	arg1	contaminants					1873:1884	Matrigel contaminants	1864:1884	Matrigel contaminants	1864:1884	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	1	24	theme	histological	185:196	arg1	characteristics					212:226	histological and molecular characteristics	185:226	histological and molecular characteristics of original tissues	185:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	5	25	theme	Matrigel	1018:1025	arg1	proteins					1027:1034	undissolved Matrigel proteins	1006:1034	undissolved Matrigel proteins	1006:1034	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	7	26	theme	minimal	1721:1727	arg1	loss					1729:1732	minimal loss	1721:1732	minimal loss of biological information	1721:1758	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	7	27	theme	other	1577:1581	arg1	methods					1587:1593	the other two methods	1573:1593	the other two methods	1573:1593	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	4	28	theme	sample	824:829	arg1	preparation					831:841	sample preparation	824:841	sample preparation	824:841	Thus, how to dissolve Matrigel matrix and recovery organoid cells efficiently is vital for sample preparation.					
34871808	6	29	theme	stable	1136:1141	arg1	labeling					1151:1158	stable isotope labeling	1136:1158	stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively	1136:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	4	30	theme	organoid	784:791	arg1	cells					793:797	recovery organoid cells	775:797	recovery organoid cells	775:797	Thus, how to dissolve Matrigel matrix and recovery organoid cells efficiently is vital for sample preparation.					
34871808	0	31	theme	In-Depth	0:7	arg1	Comparison					9:18	In-Depth Comparison	0:18	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.	0:86	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	1	32	theme	function	309:316	arg1	investigation					318:330	function investigation	309:330	function investigation	309:330	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	6	33	theme	Matrigel	1123:1130	arg1	proteomes					1110:1118	label-free proteomes	1099:1118	label-free proteomes of Matrigel	1099:1130	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	34	theme	proteomes	1110:1118	arg1	labeling					1151:1158	stable isotope labeling	1136:1158	stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively	1136:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	34	theme	proteomes	1110:1118	arg1	analysis					1087:1094	integrative analysis	1075:1094	integrative analysis of label-free proteomes of Matrigel	1075:1130	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	8	35	theme	basic	1926:1930	arg1	research					1960:1967	basic and translational proteomic research	1926:1967	research	1960:1967	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	3	36	theme	organoids	705:713	arg1	analysis					693:700	proteomic analysis	683:700	proteomic analysis of organoids in multiple ways	683:730	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	0	37	theme	Dissolving	32:41	arg1	Methods					43:49	Matrigel Dissolving Methods	23:49	Matrigel Dissolving Methods	23:49	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	7	38	theme	proteomic	1528:1536	arg1	profiles					1538:1545	proteomic profiles	1528:1545	proteomic profiles of organoids collected by the other two methods	1528:1593	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	6	39	theme	culture	1183:1189	arg1	proteomes					1191:1199	cell culture proteomes	1178:1199	cell culture proteomes of organoids collected by three methods, respectively	1178:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	1	40	theme	proteomic	254:262	arg1	models					163:168	promising ex vivo 3D culture models	134:168	promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues	134:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	1	40	theme	proteomic	254:262	arg1	profiling					264:272	proteomic profiling	254:272	proteomic profiling of organoids	254:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	3	41	theme	multiple	718:725	arg1	ways					727:730	multiple ways	718:730	multiple ways	718:730	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	5	42	theme	cell	921:924	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	42	theme	cell	921:924	arg1	solution					935:942	cell recovery solution	921:942	cell recovery solution	921:942	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	43	theme	organoids	1061:1069	arg1	profiles					1049:1056	proteomic profiles	1039:1056	proteomic profiles of organoids	1039:1069	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	44	theme	amino	1163:1167	arg1	acids					1169:1173	amino acids	1163:1173	amino acids in cell culture proteomes of organoids collected by three methods, respectively	1163:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	1	45	link	Patient-derived	88:102	arg1	organoids					104:112	Patient-derived organoids	88:112	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids	88:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	2	46	theme	murine	401:406	arg1	Matrigel					408:415	murine Matrigel	401:415	murine Matrigel (served as scaffolds and matrix)	401:448	However, organoids are usually cultured in murine Matrigel (served as scaffolds and matrix), which brings an issue to separate organoids from Matrigel.					
34871808	6	47	theme	potential	1475:1483	arg1	contaminants					1494:1505	the least potential Matrigel contaminants	1465:1505	the least potential Matrigel contaminants	1465:1505	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	48	theme	peptide	1325:1331	arg1	yield					1333:1337	the highest peptide yield	1313:1337	the highest peptide yield	1313:1337	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	8	49	theme	proteomic	1950:1958	arg1	research					1960:1967	basic and translational proteomic research	1926:1967	research	1960:1967	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	6	50	theme	cell	1420:1423	arg1	culture					1425:1431	cell culture	1420:1431	cell culture	1420:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	5	51	theme	recovery	926:933	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	51	theme	recovery	926:933	arg1	solution					935:942	cell recovery solution	921:942	cell recovery solution	921:942	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	52	theme	labeling	1393:1400	arg1	ratio					1369:1373	the highest incorporation ratio	1343:1373	the highest incorporation ratio of stable isotope labeling by amino acids in cell culture	1343:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	52	theme	labeling	1393:1400	arg1	yield					1333:1337	the highest peptide yield	1313:1337	the highest peptide yield	1313:1337	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	3	53	theme	peptides	585:592	arg1	compositions					533:544	the complex compositions	521:544	the complex compositions of Matrigel	521:556	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	3	53	theme	peptides	585:592	arg1	thousands					562:570	thousands	562:570	thousands of identical peptides shared between Matrigel and organoids	562:630	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	1	54	theme	original	231:238	arg1	tissues					240:246	original tissues	231:246	original tissues	231:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	3	55	theme	complex	525:531	arg1	compositions					533:544	the complex compositions	521:544	the complex compositions of Matrigel	521:556	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	6	56	theme	stable	1378:1383	arg1	labeling					1393:1400	stable isotope labeling	1378:1400	stable isotope labeling by amino acids in cell culture	1378:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	5	57	theme	dissolving	901:910	arg1	buffer					967:972	PBS-EDTA buffer	958:972	PBS-EDTA buffer	958:972	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	57	theme	dissolving	901:910	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	57	theme	dissolving	901:910	arg1	dispase					945:951	dispase	945:951	dispase	945:951	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	57	theme	dissolving	901:910	arg1	solution					935:942	cell recovery solution	921:942	cell recovery solution	921:942	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	58	theme	ratio	1369:1373	arg1	labels					1433:1438	the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels	1313:1438	the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%)	1313:1446	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	6	58	theme	ratio	1369:1373	arg1	%					1445:1445	97.1%	1441:1445	97.1%	1441:1445	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	5	59	theme	PBS-EDTA	958:965	arg1	buffer					967:972	PBS-EDTA buffer	958:972	PBS-EDTA buffer	958:972	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	59	theme	PBS-EDTA	958:965	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	7	60	theme	high-confidence	1614:1628	arg1	contaminants					1639:1650	312 high-confidence Matrigel contaminants	1610:1650	312 high-confidence Matrigel contaminants	1610:1650	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	7	61	with	interference	1703:1714	arg1	loss					1729:1732	minimal loss	1721:1732	minimal loss of biological information	1721:1758	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	6	62	theme	highest	1347:1353	arg1	ratio					1369:1373	the highest incorporation ratio	1343:1373	the highest incorporation ratio of stable isotope labeling by amino acids in cell culture	1343:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	1	63	theme	ex vivo	144:150	arg1	models					163:168	promising ex vivo 3D culture models	134:168	promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues	134:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	1	63	theme	ex vivo	144:150	arg1	profiling					264:272	proteomic profiling	254:272	proteomic profiling of organoids	254:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	3	64	theme	dissolved	648:656	arg1	Matrigel					658:665	insufficiently dissolved Matrigel	633:665	insufficiently dissolved Matrigel	633:665	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	5	65	theme	popular	884:890	arg1	buffer					967:972	PBS-EDTA buffer	958:972	PBS-EDTA buffer	958:972	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	65	theme	popular	884:890	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	65	theme	popular	884:890	arg1	dispase					945:951	dispase	945:951	dispase	945:951	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	65	theme	popular	884:890	arg1	solution					935:942	cell recovery solution	921:942	cell recovery solution	921:942	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	1	66	theme	culture	155:161	arg1	models					163:168	promising ex vivo 3D culture models	134:168	promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues	134:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	1	66	theme	culture	155:161	arg1	profiling					264:272	proteomic profiling	254:272	proteomic profiling of organoids	254:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	8	67	theme	organoid	1975:1982	arg1	models					1984:1989	organoid models	1975:1989	organoid models	1975:1989	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	0	68	from	Comparison	9:18	arg1	Profiling					64:72	Proteomic Profiling	54:72	Proteomic Profiling of Organoids	54:85	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	5	69	theme	undissolved	1006:1016	arg1	proteins					1027:1034	undissolved Matrigel proteins	1006:1034	undissolved Matrigel proteins	1006:1034	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	7	70	theme	biological	1737:1746	arg1	information					1748:1758	biological information	1737:1758	biological information	1737:1758	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	6	71	theme	amino	1405:1409	arg1	acids					1411:1415	amino acids	1405:1415	amino acids in cell culture	1405:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	1	72	theme	tissues	240:246	arg1	characteristics					212:226	histological and molecular characteristics	185:226	histological and molecular characteristics of original tissues	185:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	5	73	theme	proteins	1027:1034	arg1	effect					996:1001	the effect	992:1001	the effect of undissolved Matrigel proteins on proteomic profiles of organoids	992:1069	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	74	theme	isotope	1143:1149	arg1	labeling					1151:1158	stable isotope labeling	1136:1158	stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively	1136:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	2	75	from	Matrigel	500:507	arg1	organoids					485:493	separate organoids	476:493	separate organoids from Matrigel	476:507	However, organoids are usually cultured in murine Matrigel (served as scaffolds and matrix), which brings an issue to separate organoids from Matrigel.					
34871808	6	76	from	acids	1411:1415	arg1	culture					1425:1431	cell culture	1420:1431	cell culture	1420:1431	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	1	77	theme	Patient-derived	88:102	arg1	organoids					104:112	Patient-derived organoids	88:112	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids	88:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	4	78	theme	recovery	775:782	arg1	cells					793:797	recovery organoid cells	775:797	recovery organoid cells	775:797	Thus, how to dissolve Matrigel matrix and recovery organoid cells efficiently is vital for sample preparation.					
34871808	0	79	theme	Matrigel	23:30	arg1	Methods					43:49	Matrigel Dissolving Methods	23:49	Matrigel Dissolving Methods	23:49	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	8	80	theme	contaminants	1873:1884	arg1	interference					1848:1859	interference	1848:1859	interference of Matrigel contaminants	1848:1884	Together, our study identifies bioinformatics and experimental approaches to eliminate interference of Matrigel contaminants efficiently, which will be valuable for basic and translational proteomic research using organoid models.					
34871808	7	81	theme	Matrigel	1694:1701	arg1	interference					1703:1714	Matrigel interference	1694:1714	Matrigel interference with minimal loss of biological information	1694:1758	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	0	82	theme	Methods	43:49	arg1	Comparison					9:18	In-Depth Comparison	0:18	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.	0:86	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	1	83	theme	clinical	336:343	arg1	translation					345:355	clinical translation	336:355	clinical translation	336:355	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	6	84	theme	label-free	1099:1108	arg1	proteomes					1110:1118	label-free proteomes	1099:1118	label-free proteomes of Matrigel	1099:1130	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	3	85	theme	proteomic	683:691	arg1	analysis					693:700	proteomic analysis	683:700	proteomic analysis of organoids in multiple ways	683:730	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	0	86	theme	Proteomic	54:62	arg1	Profiling					64:72	Proteomic Profiling	54:72	Proteomic Profiling of Organoids	54:85	In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids.					
34871808	7	87	theme	organoids	1550:1558	arg1	profiles					1538:1545	proteomic profiles	1528:1545	proteomic profiles of organoids collected by the other two methods	1528:1593	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	2	88	theme	separate	476:483	arg1	organoids					485:493	separate organoids	476:493	separate organoids from Matrigel	476:507	However, organoids are usually cultured in murine Matrigel (served as scaffolds and matrix), which brings an issue to separate organoids from Matrigel.					
34871808	5	89	from	effect	996:1001	arg1	profiles					1049:1056	proteomic profiles	1039:1056	proteomic profiles of organoids	1039:1069	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	7	90	theme	profiles	1538:1545	arg1	analysis					1516:1523	analysis	1516:1523	analysis of proteomic profiles of organoids collected by the other two methods	1516:1593	To help analysis of proteomic profiles of organoids collected by the other two methods, we identified 312 high-confidence Matrigel contaminants, which could be filtered out to attenuate Matrigel interference with minimal loss of biological information.					
34871808	5	91	theme	proteomic	1039:1047	arg1	profiles					1049:1056	proteomic profiles	1039:1056	proteomic profiles of organoids	1039:1069	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	92	theme	cell	1178:1181	arg1	proteomes					1191:1199	cell culture proteomes	1178:1199	cell culture proteomes of organoids collected by three methods, respectively	1178:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	5	93	dep	methods	912:918	arg1	buffer					967:972	PBS-EDTA buffer	958:972	PBS-EDTA buffer	958:972	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	93	dep	methods	912:918	arg1	methods					912:918	three popular Matrigel dissolving methods	878:918	three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer)	878:973	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	93	dep	methods	912:918	arg1	dispase					945:951	dispase	945:951	dispase	945:951	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	5	93	dep	methods	912:918	arg1	solution					935:942	cell recovery solution	921:942	cell recovery solution	921:942	Here, we comprehensively compared three popular Matrigel dissolving methods (cell recovery solution, dispase, and PBS-EDTA buffer) and investigated the effect of undissolved Matrigel proteins on proteomic profiles of organoids.					
34871808	6	94	from	acids	1169:1173	arg1	proteomes					1191:1199	cell culture proteomes	1178:1199	cell culture proteomes of organoids collected by three methods, respectively	1178:1253	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	3	95	theme	Matrigel	549:556	arg1	compositions					533:544	the complex compositions	521:544	the complex compositions of Matrigel	521:556	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	3	95	theme	Matrigel	549:556	arg1	thousands					562:570	thousands	562:570	thousands of identical peptides shared between Matrigel and organoids	562:630	Because of the complex compositions of Matrigel and thousands of identical peptides shared between Matrigel and organoids, insufficiently dissolved Matrigel could influence proteomic analysis of organoids in multiple ways.					
34871808	6	96	theme	Matrigel	1485:1492	arg1	contaminants					1494:1505	the least potential Matrigel contaminants	1465:1505	the least potential Matrigel contaminants	1465:1505	By integrative analysis of label-free proteomes of Matrigel and stable isotope labeling by amino acids in cell culture proteomes of organoids collected by three methods, respectively, we found that dispase showed an optimal efficiency, with the highest peptide yield and the highest incorporation ratio of stable isotope labeling by amino acids in cell culture labels (97.1%), as well as with the least potential Matrigel contaminants.					
34871808	1	97	theme	organoids	277:285	arg1	models					163:168	promising ex vivo 3D culture models	134:168	promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues	134:246	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
34871808	1	97	theme	organoids	277:285	arg1	profiling					264:272	proteomic profiling	254:272	proteomic profiling of organoids	254:285	Patient-derived organoids recently emerged as promising ex vivo 3D culture models recapitulating histological and molecular characteristics of original tissues, thus proteomic profiling of organoids could be valuable for function investigation and clinical translation.					
33568896	6	0	theme	cyclodextrin	947:958	arg1	complex					970:976	cyclodextrin inclusion complex	947:976	cyclodextrin inclusion complex	947:976	The lipophilic drug is dissolved by the formation of cyclodextrin inclusion complex.					
33568896	2	1	theme	formulations	347:358	arg1	stability					315:323	Antimicrobial stability	301:323	Antimicrobial stability of topical ophthalmic formulations	301:358	Antimicrobial stability of topical ophthalmic formulations is especially important.					
33568896	12	2	theme	biopolymer-containing	1523:1543	arg1	samples					1545:1551	the biopolymer-containing samples	1519:1551	the biopolymer-containing samples	1519:1551	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	4	3	theme	prednisolone-containing	599:621	arg1	formulations					634:645	prednisolone-containing ophthalmic formulations	599:645	prednisolone-containing ophthalmic formulations	599:645	In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects.					
33568896	12	4	contain	had	1553:1555	arg1	samples					1545:1551	the biopolymer-containing samples	1519:1551	the biopolymer-containing samples	1519:1551	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	12	4	contain	had	1553:1555	arg2	permeability					1577:1588	significantly lower permeability	1557:1588	significantly lower permeability	1557:1588	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	4	5	theme	toxic	703:707	arg1	effects					723:729	toxic or irritative effects	703:729	toxic or irritative effects	703:729	In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects.					
33568896	12	6	theme	permeability	1499:1510	arg1	study					1512:1516	the ex vivo permeability study	1487:1516	the ex vivo permeability study	1487:1516	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	4	7	theme	irritative	712:721	arg1	effects					723:729	toxic or irritative effects	703:729	toxic or irritative effects	703:729	In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects.					
33568896	4	8	theme	expected	667:674	arg1	permeation					684:693	expected optimal permeation	667:693	expected optimal permeation without toxic or irritative effects	667:729	In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects.					
33568896	11	9	theme	suspension	1450:1459	arg1	formulation					1461:1471	suspension formulation	1450:1471	suspension formulation	1450:1471	The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation.					
33568896	0	10	theme	Drop	118:121	arg1	Formulations					123:134	Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations	76:134	Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations	76:134	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	13	11	theme	frequent	1726:1733	arg1	administration					1735:1748	less frequent administration	1721:1748	less frequent administration	1721:1748	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	12	12	theme	ex	1491:1492	arg1	study					1512:1516	the ex vivo permeability study	1487:1516	the ex vivo permeability study	1487:1516	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	6	13	theme	complex	970:976	arg1	formation					934:942	the formation	930:942	the formation of cyclodextrin inclusion complex	930:976	The lipophilic drug is dissolved by the formation of cyclodextrin inclusion complex.					
33568896	1	14	dep	structure	254:262	arg1	the					242:244	the	242:244	the	242:244	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	14	15	theme	drug	1872:1875	arg1	permeability					1877:1888	optimal drug permeability	1864:1888	optimal drug permeability	1864:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	7	16	theme	preservative	1049:1060	arg1	agent					1062:1066	an alternative preservative agent	1034:1066	an alternative preservative agent	1034:1066	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	7	16	theme	preservative	1049:1060	arg1	biopolymer					1008:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	10	17	theme	immunohistochemistry	1302:1321	arg1	assay					1323:1327	immunohistochemistry assay	1302:1327	immunohistochemistry assay	1302:1327	This was confirmed by immunohistochemistry assay.					
33568896	5	18	theme	epithelial	839:848	arg1	HCE-T					861:865	HCE-T	861:865	HCE-T	861:865	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	18	theme	epithelial	839:848	arg1	line					855:858	human corneal epithelial cell line	825:858	a human corneal epithelial cell line (HCE-T)	823:866	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	1	19	theme	BACKGROUND	137:146	arg1	penetration					169:179	BACKGROUND Optimal transcorneal penetration	137:179	BACKGROUND Optimal transcorneal penetration	137:179	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	5	20	dep	toxicity	744:751	arg1	The					740:742	The	740:742	The	740:742	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	0	21	theme	ex	51:52	arg1	Investigation					59:71	In vitro and ex vivo Investigation	38:71	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.	0:135	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	14	22	theme	ophthalmic	1835:1844	arg1	formulations					1846:1857	novel non-toxic ophthalmic formulations	1819:1857	novel non-toxic ophthalmic formulations with optimal drug permeability	1819:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	1	23	theme	transcorneal	156:167	arg1	penetration					169:179	BACKGROUND Optimal transcorneal penetration	137:179	BACKGROUND Optimal transcorneal penetration	137:179	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	11	24	dep	in	1334:1335	arg1	vitro					1337:1341	vitro	1337:1341	vitro	1337:1341	The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation.					
33568896	5	25	theme	eye	797:799	arg1	drops					801:805	prednisolone-containing eye drops	773:805	prednisolone-containing eye drops	773:805	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	14	26	theme	novel	1819:1823	arg1	formulations					1846:1857	novel non-toxic ophthalmic formulations	1819:1857	novel non-toxic ophthalmic formulations with optimal drug permeability	1819:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	0	27	dep	Cyclodextrin-Containing	76:98	arg1	Mucoadhesive					101:112	Mucoadhesive	101:112	Mucoadhesive	101:112	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	0	28	theme	Cyclodextrin-Containing	76:98	arg1	Formulations					123:134	Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations	76:134	Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations	76:134	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	7	29	theme	Zinc-containing	979:993	arg1	agent					1062:1066	an alternative preservative agent	1034:1066	an alternative preservative agent	1034:1066	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	7	29	theme	Zinc-containing	979:993	arg1	biopolymer					1008:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	3	30	theme	used	427:430	arg1	irritative					471:480	irritative	471:480	irritative	471:480	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	3	30	theme	used	427:430	arg1	benzalkonium-chloride					446:466	benzalkonium-chloride	446:466	benzalkonium-chloride	446:466	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	3	30	theme	used	427:430	arg1	preservative					432:443	the mostly used preservative	416:443	the mostly used preservative	416:443	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	0	31	theme	New	0:2	arg1	Approach					4:11	New Approach	0:11	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.	0:135	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	0	32	dep	ex	51:52	arg1	vivo					54:57	vivo	54:57	vivo	54:57	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	12	33	dep	ex	1491:1492	arg1	vivo					1494:1497	vivo	1494:1497	vivo	1494:1497	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	13	34	theme	target	1644:1649	arg1	formulations					1651:1662	target formulations	1644:1662	target formulations	1644:1662	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	0	35	theme	Drug	23:26	arg1	Delivery					28:35	Ocular Drug Delivery	16:35	Ocular Drug Delivery	16:35	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	8	36	theme	benzalkonium-chloride-containing	1154:1185	arg1	samples					1187:1193	benzalkonium-chloride-containing samples	1154:1193	benzalkonium-chloride-containing samples	1154:1193	RESULTS As the results show, benzalkonium-chloride-containing samples were toxic on HCE-T cells.					
33568896	3	37	theme	epithelial	503:512	arg1	cells					514:518	corneal epithelial cells	495:518	corneal epithelial cells	495:518	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	14	38	with	formulations	1846:1857	arg1	permeability					1877:1888	optimal drug permeability	1864:1888	optimal drug permeability	1864:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	11	39	theme	in	1334:1335	arg1	permeability					1343:1354	The in vitro permeability	1330:1354	The in vitro permeability	1330:1354	The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation.					
33568896	11	39	theme	in	1334:1335	arg1	higher					1374:1379	higher	1374:1379	higher	1374:1379	The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation.					
33568896	4	40	theme	ophthalmic	623:632	arg1	formulations					634:645	prednisolone-containing ophthalmic formulations	599:645	prednisolone-containing ophthalmic formulations	599:645	In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects.					
33568896	5	41	dep	ex	872:873	arg1	vivo					875:878	vivo	875:878	vivo	875:878	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	0	42	theme	In	38:39	arg1	Investigation					59:71	In vitro and ex vivo Investigation	38:71	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.	0:135	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	6	43	theme	inclusion	960:968	arg1	complex					970:976	cyclodextrin inclusion complex	947:976	cyclodextrin inclusion complex	947:976	The lipophilic drug is dissolved by the formation of cyclodextrin inclusion complex.					
33568896	1	44	theme	complex	246:252	arg1	structure					254:262	complex structure	246:262	complex structure	246:262	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	12	45	theme	lower	1571:1575	arg1	permeability					1577:1588	significantly lower permeability	1557:1588	significantly lower permeability	1557:1588	According to the ex vivo permeability study, the biopolymer-containing samples had significantly lower permeability.					
33568896	2	46	theme	ophthalmic	336:345	arg1	formulations					347:358	topical ophthalmic formulations	328:358	topical ophthalmic formulations	328:358	Antimicrobial stability of topical ophthalmic formulations is especially important.					
33568896	3	47	theme	non-toxic	538:546	arg1	agents					563:568	novel non-toxic, antimicrobial agents	532:568	novel non-toxic, antimicrobial agents	532:568	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	5	48	theme	human	825:829	arg1	HCE-T					861:865	HCE-T	861:865	HCE-T	861:865	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	48	theme	human	825:829	arg1	line					855:858	human corneal epithelial cell line	825:858	a human corneal epithelial cell line (HCE-T)	823:866	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	2	49	theme	Antimicrobial	301:313	arg1	stability					315:323	Antimicrobial stability	301:323	Antimicrobial stability of topical ophthalmic formulations	301:358	Antimicrobial stability of topical ophthalmic formulations is especially important.					
33568896	0	50	theme	Eye	114:116	arg1	Formulations					123:134	Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations	76:134	Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations	76:134	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	5	51	theme	ex	872:873	arg1	model					887:891	ex vivo cornea model	872:891	ex vivo cornea model	872:891	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	0	52	theme	Formulations	123:134	arg1	Investigation					59:71	In vitro and ex vivo Investigation	38:71	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.	0:135	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	5	53	theme	cornea	880:885	arg1	model					887:891	ex vivo cornea model	872:891	ex vivo cornea model	872:891	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	4	54	theme	optimal	676:682	arg1	permeation					684:693	expected optimal permeation	667:693	expected optimal permeation without toxic or irritative effects	667:729	In this study, prednisolone-containing ophthalmic formulations were developed with expected optimal permeation without toxic or irritative effects.					
33568896	5	55	dep	METHODS	732:738	arg1	METHODS					732:738	METHODS	732:738	METHODS The toxicity and permeability of prednisolone-containing eye drops	732:805	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	55	dep	METHODS	732:738	arg1	permeability					757:768	permeability	757:768	permeability	757:768	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	55	dep	METHODS	732:738	arg1	toxicity					744:751	toxicity	744:751	toxicity	744:751	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	3	56	dep	non-toxic	538:546	arg1	antimicrobial					549:561	antimicrobial	549:561	antimicrobial	549:561	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	13	57	with	application	1704:1714	arg1	administration					1735:1748	less frequent administration	1721:1748	less frequent administration	1721:1748	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	14	58	theme	optimal	1864:1870	arg1	permeability					1877:1888	optimal drug permeability	1864:1888	optimal drug permeability	1864:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	1	59	theme	ocular	198:203	arg1	therapy					205:211	ocular therapy	198:211	ocular therapy	198:211	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	7	60	theme	alternative	1037:1047	arg1	agent					1062:1066	an alternative preservative agent	1034:1066	an alternative preservative agent	1034:1066	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	7	60	theme	alternative	1037:1047	arg1	biopolymer					1008:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	1	61	theme	Optimal	148:154	arg1	penetration					169:179	BACKGROUND Optimal transcorneal penetration	137:179	BACKGROUND Optimal transcorneal penetration	137:179	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	0	62	from	Approach	4:11	arg1	Delivery					28:35	Ocular Drug Delivery	16:35	Ocular Drug Delivery	16:35	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	11	63	with	formulations	1384:1395	arg1	complex					1428:1434	prednisolone-cyclodextrin complex	1402:1434	prednisolone-cyclodextrin complex	1402:1434	The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation.					
33568896	5	64	theme	prednisolone-containing	773:795	arg1	drops					801:805	prednisolone-containing eye drops	773:805	prednisolone-containing eye drops	773:805	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	14	65	theme	non-toxic	1825:1833	arg1	formulations					1846:1857	novel non-toxic ophthalmic formulations	1819:1857	novel non-toxic ophthalmic formulations with optimal drug permeability	1819:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	3	66	theme	corneal	495:501	arg1	cells					514:518	corneal epithelial cells	495:518	corneal epithelial cells	495:518	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	5	67	theme	drops	801:805	arg1	METHODS					732:738	METHODS	732:738	METHODS The toxicity and permeability of prednisolone-containing eye drops	732:805	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	67	theme	drops	801:805	arg1	permeability					757:768	permeability	757:768	permeability	757:768	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	67	theme	drops	801:805	arg1	toxicity					744:751	toxicity	744:751	toxicity	744:751	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	13	68	theme	prolonged	1665:1673	arg1	attribute					1631:1639	the mucoadhesive attribute	1614:1639	the mucoadhesive attribute of target formulations	1614:1662	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	13	68	theme	prolonged	1665:1673	arg1	absorption					1675:1684	prolonged absorption	1665:1684	prolonged absorption	1665:1684	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	14	69	theme	innovative	1794:1803	arg1	compositions					1777:1788	the compositions	1773:1788	the compositions	1773:1788	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	14	69	theme	innovative	1794:1803	arg1	approaches					1805:1814	innovative approaches	1794:1814	innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability	1794:1888	It can be stated that the compositions are innovative approaches as novel non-toxic ophthalmic formulations with optimal drug permeability.					
33568896	7	70	theme	mucoadhesive	995:1006	arg1	agent					1062:1066	an alternative preservative agent	1034:1066	an alternative preservative agent	1034:1066	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	7	70	theme	mucoadhesive	995:1006	arg1	biopolymer					1008:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer	979:1017	Zinc-containing mucoadhesive biopolymer was applied as an alternative preservative agent, whose toxicity was compared with benzalkonium-chloride.					
33568896	3	71	theme	previous	398:405	arg1	studies					407:413	previous studies	398:413	previous studies	398:413	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
33568896	11	72	theme	prednisolone-cyclodextrin	1402:1426	arg1	complex					1428:1434	prednisolone-cyclodextrin complex	1402:1434	prednisolone-cyclodextrin complex	1402:1434	The in vitro permeability was significantly higher in formulations with prednisolone-cyclodextrin complex compared with suspension formulation.					
33568896	0	73	theme	Ocular	16:21	arg1	Delivery					28:35	Ocular Drug Delivery	16:35	Ocular Drug Delivery	16:35	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	1	74	theme	defensive	268:276	arg1	mechanisms					278:287	defensive mechanisms	268:287	defensive mechanisms	268:287	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	5	75	theme	corneal	831:837	arg1	HCE-T					861:865	HCE-T	861:865	HCE-T	861:865	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	75	theme	corneal	831:837	arg1	line					855:858	human corneal epithelial cell line	825:858	a human corneal epithelial cell line (HCE-T)	823:866	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	6	76	theme	lipophilic	898:907	arg1	drug					909:912	The lipophilic drug	894:912	The lipophilic drug	894:912	The lipophilic drug is dissolved by the formation of cyclodextrin inclusion complex.					
33568896	0	77	dep	In	38:39	arg1	vitro					41:45	vitro	41:45	vitro	41:45	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	13	78	theme	formulations	1651:1662	arg1	attribute					1631:1639	the mucoadhesive attribute	1614:1639	the mucoadhesive attribute of target formulations	1614:1662	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	13	78	theme	formulations	1651:1662	arg1	absorption					1675:1684	prolonged absorption	1665:1684	prolonged absorption	1665:1684	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	5	79	theme	cell	850:853	arg1	HCE-T					861:865	HCE-T	861:865	HCE-T	861:865	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	5	79	theme	cell	850:853	arg1	line					855:858	human corneal epithelial cell line	825:858	a human corneal epithelial cell line (HCE-T)	823:866	METHODS The toxicity and permeability of prednisolone-containing eye drops were studied on a human corneal epithelial cell line (HCE-T) and ex vivo cornea model.					
33568896	1	80	theme	eye	296:298	arg1	mechanisms					278:287	defensive mechanisms	268:287	defensive mechanisms	268:287	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	1	80	theme	eye	296:298	arg1	structure					254:262	complex structure	246:262	complex structure	246:262	BACKGROUND Optimal transcorneal penetration is necessary for ocular therapy; meanwhile, it is limited by the complex structure and defensive mechanisms of the eye.					
33568896	2	81	theme	topical	328:334	arg1	formulations					347:358	topical ophthalmic formulations	328:358	topical ophthalmic formulations	328:358	Antimicrobial stability of topical ophthalmic formulations is especially important.					
33568896	8	82	theme	HCE-T	1209:1213	arg1	cells					1215:1219	HCE-T cells	1209:1219	HCE-T cells	1209:1219	RESULTS As the results show, benzalkonium-chloride-containing samples were toxic on HCE-T cells.					
33568896	13	83	theme	mucoadhesive	1618:1629	arg1	attribute					1631:1639	the mucoadhesive attribute	1614:1639	the mucoadhesive attribute of target formulations	1614:1662	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	13	83	theme	mucoadhesive	1618:1629	arg1	absorption					1675:1684	prolonged absorption	1665:1684	prolonged absorption	1665:1684	CONCLUSION Considering the mucoadhesive attribute of target formulations, prolonged absorption is expected after application with less frequent administration.					
33568896	0	84	dep	Approach	4:11	arg1	Investigation					59:71	In vitro and ex vivo Investigation	38:71	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.	0:135	New Approach in Ocular Drug Delivery: In vitro and ex vivo Investigation of Cyclodextrin-Containing, Mucoadhesive Eye Drop Formulations.					
33568896	9	85	theme	cell	1247:1250	arg1	damage					1252:1257	no cell damage	1244:1257	no cell damage	1244:1257	The biopolymer caused no cell damage after the treatment.					
33568896	3	86	theme	novel	532:536	arg1	agents					563:568	novel non-toxic, antimicrobial agents	532:568	novel non-toxic, antimicrobial agents	532:568	According to previous studies, the mostly used preservative, benzalkonium-chloride is irritative and toxic on corneal epithelial cells; therefore, novel non-toxic, antimicrobial agents are required.					
32956031	6	0	theme	unidentified	868:879	arg1	phosphatidylethanolamine					764:787	phosphatidylethanolamine	764:787	phosphatidylethanolamine	764:787	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	0	theme	unidentified	868:879	arg1	aminolipids					881:891	two unidentified aminolipids	864:891	two unidentified aminolipids	864:891	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	5	1	theme	strains	593:599	arg1	anteiso-C15 					651:662	anteiso-C15 	651:662	anteiso-C15 	651:662	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	1	theme	strains	593:599	arg1	acids					576:580	The major fatty acids	560:580	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107	560:630	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	1	theme	strains	593:599	arg1	%					587:587	>10 %	583:587	>10 %	583:587	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	1	2	theme	hot	135:137	arg1	spring					139:144	a hot spring	133:144	a hot spring	133:144	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	8	3	theme	analyses	1420:1427	arg1	properties					1380:1389	their morphological and physiological properties	1342:1389	their morphological and physiological properties	1342:1389	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	8	3	theme	analyses	1420:1427	arg1	results					1396:1402	results	1396:1402	results	1396:1402	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	8	4	dep	sp	1581:1582	arg1	nov.					1575:1578	the name Seramator thermalis gen. nov.	1541:1578	the name Seramator thermalis gen. nov.	1541:1578	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	5	5	theme	major	564:568	arg1	anteiso-C15 					651:662	anteiso-C15 	651:662	anteiso-C15 	651:662	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	5	theme	major	564:568	arg1	acids					576:580	The major fatty acids	560:580	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107	560:630	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	5	theme	major	564:568	arg1	%					587:587	>10 %	583:587	>10 %	583:587	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	9	6	theme	strain	1596:1601	arg1	nov					1585:1587	nov	1585:1587	nov	1585:1587	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	9	6	theme	strain	1596:1601	arg1	15753T					1636:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	8	7	dep	strains	1430:1436	arg1	strains					1430:1436	strains	1430:1436	strains SYSU GA16112T and SYSU GA16107	1430:1467	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	8	7	dep	strains	1430:1436	arg1	SYSU					1456:1459	SYSU	1456:1459	SYSU	1456:1459	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	8	7	dep	strains	1430:1436	arg1	GA16112T					1443:1450	GA16112T	1443:1450	GA16112T	1443:1450	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	3	8	theme	Phylogenetic	313:324	arg1	analyses					326:333	Phylogenetic analyses	313:333	Phylogenetic analyses based on 16S rRNA gene sequences	313:366	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	4	9	theme	strains	468:474	arg1	Cells					459:463	Cells	459:463	Cells of strains SYSU GA16112T and SYSU GA16107	459:505	Cells of strains SYSU GA16112T and SYSU GA16107 were Gram-stain-negative, rod-shaped and non-motile.					
32956031	9	10	theme	type	1591:1594	arg1	nov					1585:1587	nov	1585:1587	nov	1585:1587	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	9	10	theme	type	1591:1594	arg1	15753T					1636:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	5	11	theme	fatty	570:574	arg1	anteiso-C15 					651:662	anteiso-C15 	651:662	anteiso-C15 	651:662	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	11	theme	fatty	570:574	arg1	acids					576:580	The major fatty acids	560:580	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107	560:630	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	11	theme	fatty	570:574	arg1	%					587:587	>10 %	583:587	>10 %	583:587	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	7	12	theme	nucleotide	1281:1290	arg1	%					1330:1330	99.99 %	1324:1330	99.99 %	1324:1330	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	12	theme	nucleotide	1281:1290	arg1	value					1301:1305	the average nucleotide identity value	1269:1305	the average nucleotide identity value between them	1269:1318	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	6	13	theme	strain	941:946	arg1	SYSU					948:951	strain SYSU GA16107	941:959	strain SYSU GA16107	941:959	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	5	14	dep	identified	637:646	arg1	anteiso-C17 					671:682	anteiso-C17 	671:682	anteiso-C17 	671:682	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	14	dep	identified	637:646	arg1	 0					664:665	 0	664:665	 0	664:665	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	8	15	theme	novel	1499:1503	arg1	species					1505:1511	a novel species	1497:1511	a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp	1497:1582	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	7	16	dep	strains	1171:1177	arg1	SYSU					1197:1200	SYSU	1197:1200	SYSU	1197:1200	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	16	dep	strains	1171:1177	arg1	GA16112T					1184:1191	GA16112T	1184:1191	GA16112T	1184:1191	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	16	dep	strains	1171:1177	arg1	strains					1171:1177	strains SYSU GA16112T and SYSU GA16107	1171:1208	strains SYSU GA16112T and SYSU GA16107	1171:1208	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	6	17	theme	GA16112T	731:738	arg1	profile					708:714	The polar lipid profile	692:714	The polar lipid profile of strain SYSU GA16112T	692:738	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	0	18	theme	Seramator	0:8	arg1	nov.					25:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov., sp.					
32956031	6	19	theme	unidentified	831:842	arg1	phosphatidylethanolamine					764:787	phosphatidylethanolamine	764:787	phosphatidylethanolamine	764:787	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	19	theme	unidentified	831:842	arg1	phosphoglycolipids					844:861	two unidentified phosphoglycolipids	827:861	two unidentified phosphoglycolipids	827:861	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	9	20	theme	SYSU	1603:1606	arg1	nov					1585:1587	nov	1585:1587	nov	1585:1587	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	9	20	theme	SYSU	1603:1606	arg1	15753T					1636:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	6	21	theme	SYSU	726:729	arg1	GA16112T					731:738	strain SYSU GA16112T	719:738	strain SYSU GA16112T	719:738	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	1	22	theme	novel	43:47	arg1	member					80:85	a novel cellulose- and xylan-degrading member	41:85	a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae	41:117	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	1	22	theme	novel	43:47	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	5	23	theme	 0	684:685	arg1	3OH					687:689	 0 3OH	684:689	 0 and anteiso-C17 : 0 3OH	664:689	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	7	24	theme	strains	1171:1177	arg1	contents					1159:1166	The genomic DNA G+C contents	1139:1166	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107	1139:1208	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	24	theme	strains	1171:1177	arg1	%					1248:1248	41.90 and 41.89 %	1232:1248	41.90 and 41.89 %	1232:1248	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	6	25	theme	strain	719:724	arg1	GA16112T					731:738	strain SYSU GA16112T	719:738	strain SYSU GA16112T	719:738	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	1	26	theme	cellulose-	49:58	arg1	member					80:85	a novel cellulose- and xylan-degrading member	41:85	a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae	41:117	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	1	26	theme	cellulose-	49:58	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	6	27	theme	unidentified	1037:1048	arg1	phosphatidylethanolamine					974:997	phosphatidylethanolamine	974:997	phosphatidylethanolamine	974:997	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	27	theme	unidentified	1037:1048	arg1	aminophospholipids					1050:1067	three unidentified aminophospholipids	1031:1067	three unidentified aminophospholipids	1031:1067	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	3	28	theme	16S	344:346	arg1	sequences					358:366	16S rRNA gene sequences	344:366	16S rRNA gene sequences	344:366	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	8	29	theme	genus	1524:1528	arg1	species					1505:1511	a novel species	1497:1511	a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp	1497:1582	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	9	30	theme	1.5281T=KCTC	1623:1634	arg1	nov					1585:1587	nov	1585:1587	nov	1585:1587	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	9	30	theme	1.5281T=KCTC	1623:1634	arg1	15753T					1636:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	8	31	theme	thermalis	1560:1568	arg1	nov.					1575:1578	the name Seramator thermalis gen. nov.	1541:1578	the name Seramator thermalis gen. nov.	1541:1578	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	7	32	theme	identity	1292:1299	arg1	%					1330:1330	99.99 %	1324:1330	99.99 %	1324:1330	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	32	theme	identity	1292:1299	arg1	value					1301:1305	the average nucleotide identity value	1269:1305	the average nucleotide identity value between them	1269:1318	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	3	33	theme	rRNA	348:351	arg1	sequences					358:366	16S rRNA gene sequences	344:366	16S rRNA gene sequences	344:366	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	8	34	theme	novel	1518:1522	arg1	genus					1524:1528	a novel genus	1516:1528	a novel genus	1516:1528	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	1	35	theme	xylan-degrading	64:78	arg1	member					80:85	a novel cellulose- and xylan-degrading member	41:85	a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae	41:117	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	1	35	theme	xylan-degrading	64:78	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	6	36	theme	unidentified	794:805	arg1	phosphatidylethanolamine					764:787	phosphatidylethanolamine	764:787	phosphatidylethanolamine	764:787	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	36	theme	unidentified	794:805	arg1	aminophospholipids					807:824	two unidentified aminophospholipids	790:824	two unidentified aminophospholipids	790:824	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	5	37	dep	strains	593:599	arg1	GA16112T					606:613	GA16112T	606:613	GA16112T	606:613	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	37	dep	strains	593:599	arg1	GA16107					624:630	GA16107	624:630	GA16107	624:630	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	5	37	dep	strains	593:599	arg1	strains					593:599	strains SYSU GA16112T and SYSU GA16107	593:630	strains SYSU GA16112T and SYSU GA16107	593:630	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	6	38	theme	lipid	702:706	arg1	profile					708:714	The polar lipid profile	692:714	The polar lipid profile of strain SYSU GA16112T	692:738	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	8	39	theme	morphological	1348:1360	arg1	properties					1380:1389	their morphological and physiological properties	1342:1389	their morphological and physiological properties	1342:1389	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	8	40	theme	physiological	1366:1378	arg1	properties					1380:1389	their morphological and physiological properties	1342:1389	their morphological and physiological properties	1342:1389	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	7	41	theme	average	1273:1279	arg1	%					1330:1330	99.99 %	1324:1330	99.99 %	1324:1330	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	41	theme	average	1273:1279	arg1	value					1301:1305	the average nucleotide identity value	1269:1305	the average nucleotide identity value between them	1269:1318	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	0	42	theme	gen.	20:23	arg1	nov.					25:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov., sp.					
32956031	2	43	theme	hot	243:245	arg1	spring					247:252	a hot spring	241:252	a hot spring in Tengchong County, Yunnan Province, south-west PR China	241:310	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	6	44	theme	polar	1017:1021	arg1	lipids					1023:1028	two unidentified polar lipids	1000:1028	two unidentified polar lipids	1000:1028	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	44	theme	polar	1017:1021	arg1	phosphatidylethanolamine					974:997	phosphatidylethanolamine	974:997	phosphatidylethanolamine	974:997	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	7	45	theme	genomic	1143:1149	arg1	contents					1159:1166	The genomic DNA G+C contents	1139:1166	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107	1139:1208	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	45	theme	genomic	1143:1149	arg1	%					1248:1248	41.90 and 41.89 %	1232:1248	41.90 and 41.89 %	1232:1248	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	6	46	theme	unidentified	1114:1125	arg1	phosphatidylethanolamine					974:997	phosphatidylethanolamine	974:997	phosphatidylethanolamine	974:997	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	46	theme	unidentified	1114:1125	arg1	aminolipid					1127:1136	one unidentified aminolipid	1110:1136	one unidentified aminolipid	1110:1136	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	2	47	theme	anaerobic	151:159	arg1	bacteria					161:168	Two anaerobic bacteria	147:168	Two anaerobic bacteria	147:168	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	47	theme	anaerobic	151:159	arg1	strains					182:188	designated strains SYSU GA16112T and SYSU GA16107	171:219	designated strains SYSU GA16112T and SYSU GA16107	171:219	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	3	48	dep	strains	380:386	arg1	GA16112T					393:400	GA16112T	393:400	GA16112T	393:400	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	3	48	dep	strains	380:386	arg1	GA16107					411:417	GA16107	411:417	GA16107	411:417	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	3	48	dep	strains	380:386	arg1	strains					380:386	strains	380:386	strains SYSU GA16112T and SYSU GA16107	380:417	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	6	49	theme	unidentified	1004:1015	arg1	lipids					1023:1028	two unidentified polar lipids	1000:1028	two unidentified polar lipids	1000:1028	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	49	theme	unidentified	1004:1015	arg1	phosphatidylethanolamine					974:997	phosphatidylethanolamine	974:997	phosphatidylethanolamine	974:997	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	7	50	theme	DNA	1151:1153	arg1	contents					1159:1166	The genomic DNA G+C contents	1139:1166	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107	1139:1208	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	50	theme	DNA	1151:1153	arg1	%					1248:1248	41.90 and 41.89 %	1232:1248	41.90 and 41.89 %	1232:1248	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	6	51	theme	polar	914:918	arg1	phosphatidylethanolamine					764:787	phosphatidylethanolamine	764:787	phosphatidylethanolamine	764:787	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	51	theme	polar	914:918	arg1	lipid					920:924	one unidentified polar lipid	897:924	one unidentified polar lipid	897:924	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	7	52	theme	G+C	1155:1157	arg1	contents					1159:1166	The genomic DNA G+C contents	1139:1166	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107	1139:1208	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	7	52	theme	G+C	1155:1157	arg1	%					1248:1248	41.90 and 41.89 %	1232:1248	41.90 and 41.89 %	1232:1248	The genomic DNA G+C contents of strains SYSU GA16112T and SYSU GA16107 were determined to be 41.90 and 41.89 %, respectively, and the average nucleotide identity value between them was 99.99 %.					
32956031	6	53	theme	polar	696:700	arg1	profile					708:714	The polar lipid profile	692:714	The polar lipid profile of strain SYSU GA16112T	692:738	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	3	54	theme	gene	353:356	arg1	sequences					358:366	16S rRNA gene sequences	344:366	16S rRNA gene sequences	344:366	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	4	55	dep	strains	468:474	arg1	GA16112T					481:488	GA16112T	481:488	GA16112T	481:488	Cells of strains SYSU GA16112T and SYSU GA16107 were Gram-stain-negative, rod-shaped and non-motile.					
32956031	4	55	dep	strains	468:474	arg1	GA16107					499:505	GA16107	499:505	GA16107	499:505	Cells of strains SYSU GA16112T and SYSU GA16107 were Gram-stain-negative, rod-shaped and non-motile.					
32956031	4	55	dep	strains	468:474	arg1	strains					468:474	strains SYSU GA16112T and SYSU GA16107	468:505	strains SYSU GA16112T and SYSU GA16107	468:505	Cells of strains SYSU GA16112T and SYSU GA16107 were Gram-stain-negative, rod-shaped and non-motile.					
32956031	0	56	dep	sp	31:32	arg1	nov.					25:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov., sp.					
32956031	8	57	theme	name	1545:1548	arg1	nov.					1575:1578	the name Seramator thermalis gen. nov.	1541:1578	the name Seramator thermalis gen. nov.	1541:1578	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	9	58	theme	GA16112T=CGMCC	1608:1621	arg1	nov					1585:1587	nov	1585:1587	nov	1585:1587	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	9	58	theme	GA16112T=CGMCC	1608:1621	arg1	15753T					1636:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T	1591:1641	nov. (type strain SYSU GA16112T=CGMCC 1.5281T=KCTC 15753T) is proposed.					
32956031	1	59	theme	family	94:99	arg1	Dysgonamonadaceae					101:117	the family Dysgonamonadaceae	90:117	the family Dysgonamonadaceae	90:117	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	1	60	theme	Dysgonamonadaceae	101:117	arg1	member					80:85	a novel cellulose- and xylan-degrading member	41:85	a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae	41:117	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	1	60	theme	Dysgonamonadaceae	101:117	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel cellulose- and xylan-degrading member of the family Dysgonamonadaceae isolated from a hot spring.					
32956031	2	61	attach	isolated	227:234	arg1	spring					247:252	a hot spring	241:252	a hot spring in Tengchong County, Yunnan Province, south-west PR China	241:310	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	61	attach	isolated	227:234	arg2	strains					182:188	designated strains SYSU GA16112T and SYSU GA16107	171:219	designated strains SYSU GA16112T and SYSU GA16107	171:219	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	61	attach	isolated	227:234	arg2	bacteria					161:168	Two anaerobic bacteria	147:168	Two anaerobic bacteria	147:168	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	62	theme	designated	171:180	arg1	GA16112T					195:202	GA16112T	195:202	GA16112T	195:202	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	62	theme	designated	171:180	arg1	GA16107					213:219	GA16107	213:219	GA16107	213:219	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	62	theme	designated	171:180	arg1	strains					182:188	designated strains SYSU GA16112T and SYSU GA16107	171:219	designated strains SYSU GA16112T and SYSU GA16107	171:219	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	62	theme	designated	171:180	arg1	bacteria					161:168	Two anaerobic bacteria	147:168	Two anaerobic bacteria	147:168	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	3	63	theme	family	433:438	arg1	Dysgonamonadaceae					440:456	the family Dysgonamonadaceae	429:456	the family Dysgonamonadaceae	429:456	Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112T and SYSU GA16107 belong to the family Dysgonamonadaceae.					
32956031	8	64	theme	Seramator	1550:1558	arg1	nov.					1575:1578	the name Seramator thermalis gen. nov.	1541:1578	the name Seramator thermalis gen. nov.	1541:1578	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	6	65	theme	unidentified	1074:1085	arg1	phosphoglycolipids					1087:1104	two unidentified phosphoglycolipids	1070:1104	two unidentified phosphoglycolipids	1070:1104	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	65	theme	unidentified	1074:1085	arg1	phosphatidylethanolamine					974:997	phosphatidylethanolamine	974:997	phosphatidylethanolamine	974:997	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	8	66	theme	phylogenetic	1407:1418	arg1	analyses					1420:1427	phylogenetic analyses	1407:1427	phylogenetic analyses	1407:1427	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	2	67	from	spring	247:252	arg1	County					267:272	County	267:272	County	267:272	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	67	from	spring	247:252	arg1	Province					282:289	Province	282:289	Province	282:289	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	67	from	spring	247:252	arg1	China					306:310	China	306:310	China	306:310	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	5	68	dep	 0	664:665	arg1	3OH					687:689	 0 3OH	684:689	 0 and anteiso-C17 : 0 3OH	664:689	The major fatty acids (>10 %) of strains SYSU GA16112T and SYSU GA16107 were identified as anteiso-C15 : 0 and anteiso-C17 : 0 3OH.					
32956031	8	69	theme	gen.	1570:1573	arg1	nov.					1575:1578	the name Seramator thermalis gen. nov.	1541:1578	the name Seramator thermalis gen. nov.	1541:1578	Based on their morphological and physiological properties, and results of phylogenetic analyses, strains SYSU GA16112T and SYSU GA16107 are considered to represent a novel species of a novel genus, for which the name Seramator thermalis gen. nov., sp.					
32956031	0	70	theme	thermalis	10:18	arg1	nov.					25:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov.	0:28	Seramator thermalis gen. nov., sp.					
32956031	6	71	theme	unidentified	901:912	arg1	phosphatidylethanolamine					764:787	phosphatidylethanolamine	764:787	phosphatidylethanolamine	764:787	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	6	71	theme	unidentified	901:912	arg1	lipid					920:924	one unidentified polar lipid	897:924	one unidentified polar lipid	897:924	The polar lipid profile of strain SYSU GA16112T was found to consist of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phosphoglycolipids, two unidentified aminolipids and one unidentified polar lipid, while that of strain SYSU GA16107 consisted of phosphatidylethanolamine, two unidentified polar lipids, three unidentified aminophospholipids, two unidentified phosphoglycolipids and one unidentified aminolipid.					
32956031	2	72	dep	strains	182:188	arg1	GA16112T					195:202	GA16112T	195:202	GA16112T	195:202	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	72	dep	strains	182:188	arg1	GA16107					213:219	GA16107	213:219	GA16107	213:219	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32956031	2	72	dep	strains	182:188	arg1	strains					182:188	designated strains SYSU GA16112T and SYSU GA16107	171:219	designated strains SYSU GA16112T and SYSU GA16107	171:219	Two anaerobic bacteria, designated strains SYSU GA16112T and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China.					
32323414	8	0	theme	good	1653:1656	arg1	biocompatibility					1658:1673	good biocompatibility	1653:1673	good biocompatibility of HAP/CS/Gr/Gent coating	1653:1699	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	7	1	theme	antibacterial	1149:1161	arg1	activity					1163:1170	the antibacterial activity	1145:1170	the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units)	1145:1518	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	4	2	theme	scanning	656:663	arg1	microscopy					674:683	field emission scanning electron microscopy	641:683	field emission scanning electron microscopy	641:683	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	1	3	theme	graphene	209:216	arg1	coating					244:250	graphene (Gr)-reinforced composite coating	209:250	graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent)	209:329	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	9	4	theme	HAP/CS/Gr/Gent	1730:1743	arg1	material					1794:1801	a prospective material	1780:1801	a prospective material for bone tissue engineering	1780:1829	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	4	theme	HAP/CS/Gr/Gent	1730:1743	arg1	implant					1848:1854	a hard tissue implant	1834:1854	a hard tissue implant	1834:1854	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	4	theme	HAP/CS/Gr/Gent	1730:1743	arg1	coating					1745:1751	electrodeposited HAP/CS/Gr/Gent coating	1713:1751	electrodeposited HAP/CS/Gr/Gent coating on Ti	1713:1757	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	1	5	theme	Gr	219:220	arg1	coating					244:250	graphene (Gr)-reinforced composite coating	209:250	graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent)	209:329	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	9	6	from	coating	1745:1751	arg1	Ti					1756:1757	Ti	1756:1757	Ti	1756:1757	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	5	7	theme	Obtained	855:862	arg1	coatings					864:871	Obtained coatings	855:871	Obtained coatings	855:871	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	4	8	theme	field	641:645	arg1	microscopy					674:683	field emission scanning electron microscopy	641:683	field emission scanning electron microscopy	641:683	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	3	9	theme	biological	571:580	arg1	characterization					582:597	biological characterization	571:597	biological characterization	571:597	The influence of graphene was examined through detailed physicochemical and biological characterization.					
32323414	0	10	from	applications	91:102	arg1	engineering					119:129	bone tissue engineering	107:129	bone tissue engineering	107:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	7	11	theme	logarithmic	1433:1443	arg1	units					1445:1449	>3 logarithmic units	1430:1449	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	5	12	theme	interfacial	936:946	arg1	interaction					948:958	strong interfacial interaction	929:958	strong interfacial interaction between HAP, CS, and Gr	929:982	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	8	13	theme	low	1541:1543	arg1	viability					1568:1576	75% cell viability	1559:1576	75% cell viability	1559:1576	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	8	13	theme	low	1541:1543	arg1	cytotoxicity					1545:1556	low cytotoxicity	1541:1556	low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines	1541:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	7	14	theme	cells	1407:1411	arg1	reduction					1420:1428	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	14	theme	cells	1407:1411	arg1	aureus					1378:1383	Staphylococcus aureus	1363:1383	Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units)	1363:1450	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	4	15	theme	thermogravimetric	686:702	arg1	analysis					704:711	thermogravimetric analysis	686:711	thermogravimetric analysis	686:711	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	7	16	theme	diffusion	1275:1283	arg1	testing					1285:1291	agar diffusion testing	1270:1291	agar diffusion testing	1270:1291	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	2	17	theme	time	458:461	arg1	12 min					463:468	deposition time 12 min	447:468	deposition time 12 min	447:468	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	2	17	theme	time	458:461	arg1	5 V					442:444	5 V	442:444	5 V	442:444	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	0	18	theme	bone	107:110	arg1	engineering					119:129	bone tissue engineering	107:129	bone tissue engineering	107:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	2	19	dep	voltage	433:439	arg1	12 min					463:468	deposition time 12 min	447:468	deposition time 12 min	447:468	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	2	19	dep	voltage	433:439	arg1	5 V					442:444	5 V	442:444	5 V	442:444	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	4	20	from	formation	787:795	arg1	Ti					851:852	Ti	851:852	Ti	851:852	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	8	21	theme	%	1561:1561	arg1	viability					1568:1576	75% cell viability	1559:1576	75% cell viability	1559:1576	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	8	21	theme	%	1561:1561	arg1	cytotoxicity					1545:1556	low cytotoxicity	1541:1556	low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines	1541:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	1	22	theme	aqueous	337:343	arg1	suspension					345:354	aqueous suspension	337:354	aqueous suspension	337:354	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	1	23	used	used	190:193	arg2	process					159:165	Electrophoretic deposition process	132:165	Electrophoretic deposition process (EPD)	132:171	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	1	23	used	used	190:193	arg2	EPD					168:170	EPD	168:170	EPD	168:170	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	6	24	theme	specific	991:998	arg1	area					1000:1003	Large specific area	985:1003	Large specific area of graphene	985:1015	Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix.					
32323414	2	25	theme	pure	474:477	arg1	foils					488:492	pure titanium foils	474:492	pure titanium foils	474:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	2	26	from	voltage	433:439	arg1	foils					488:492	pure titanium foils	474:492	pure titanium foils	474:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	7	27	theme	activity	1234:1241	arg1	kinetics					1243:1250	antibacterial activity kinetics	1220:1250	antibacterial activity kinetics in suspension and agar diffusion testing	1220:1291	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	2	28	theme	constant	424:431	arg1	voltage					433:439	a constant voltage	422:439	a constant voltage (5 V, deposition time 12 min) on pure titanium foils	422:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	0	29	theme	Antibacterial	0:12	arg1	coating					53:59	Antibacterial graphene-based hydroxyapatite/chitosan coating	0:59	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering	0:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	7	30	theme	bactericidal	1386:1397	arg1	cells					1407:1411	bactericidal, viable cells	1386:1411	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	4	31	theme	photoelectron	750:762	arg1	analyses					764:771	X-ray photoelectron analyses	744:771	X-ray photoelectron analyses	744:771	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	0	32	theme	hydroxyapatite/chitosan	29:51	arg1	coating					53:59	Antibacterial graphene-based hydroxyapatite/chitosan coating	0:59	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering	0:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	1	33	theme	deposition	148:157	arg1	EPD					168:170	EPD	168:170	EPD	168:170	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	1	33	theme	deposition	148:157	arg1	process					159:165	Electrophoretic deposition process	132:165	Electrophoretic deposition process (EPD)	132:171	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	4	34	theme	coatings	839:846	arg1	formation					787:795	the formation	783:795	the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti	783:852	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	7	35	theme	coating	1190:1196	arg1	activity					1163:1170	the antibacterial activity	1145:1170	the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units)	1145:1518	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	36	dep	bactericidal	1386:1397	arg1	viable					1400:1405	viable	1400:1405	viable	1400:1405	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	8	37	theme	%	1605:1605	arg1	viability					1612:1620	70% cell viability	1603:1620	70% cell viability	1603:1620	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	2	38	dep	step	406:409	arg1	process					411:417	process	411:417	process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils	411:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	7	39	theme	antibacterial	1334:1346	arg1	effect					1348:1353	more pronounced antibacterial effect	1318:1353	more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units)	1318:1450	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	8	40	theme	viability	1612:1620	arg1	lines					1635:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	4	41	theme	composite	800:808	arg1	HAP/CS/Gr					810:818	composite HAP/CS/Gr	800:818	composite HAP/CS/Gr	800:818	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	8	42	theme	HAP/CS/Gr/Gent	1678:1691	arg1	coating					1693:1699	HAP/CS/Gr/Gent coating	1678:1699	HAP/CS/Gr/Gent coating	1678:1699	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	8	43	theme	tested	1623:1628	arg1	lines					1635:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	9	44	theme	bone	1807:1810	arg1	engineering					1819:1829	bone tissue engineering	1807:1829	bone tissue engineering	1807:1829	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	5	45	dep	porous	877:882	arg1	fracture-free					894:906	fracture-free	894:906	fracture-free	894:906	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	5	45	dep	porous	877:882	arg1	uniform					885:891	uniform	885:891	uniform	885:891	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	7	46	theme	logarithmic	1501:1511	arg1	units					1513:1517	<3 logarithmic units	1498:1517	<3 logarithmic units	1498:1517	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	46	theme	logarithmic	1501:1511	arg1	bacteriostatic					1482:1495	bacteriostatic	1482:1495	bacteriostatic	1482:1495	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	47	theme	Gentamicin	1107:1116	arg1	addition					1118:1125	Gentamicin addition	1107:1125	Gentamicin addition	1107:1125	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	5	48	theme	porous	877:882	arg1	surfaces					908:915	porous, uniform, fracture-free surfaces	877:915	porous, uniform, fracture-free surfaces	877:915	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	9	49	theme	electrodeposited	1713:1728	arg1	material					1794:1801	a prospective material	1780:1801	a prospective material for bone tissue engineering	1780:1829	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	49	theme	electrodeposited	1713:1728	arg1	implant					1848:1854	a hard tissue implant	1834:1854	a hard tissue implant	1834:1854	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	49	theme	electrodeposited	1713:1728	arg1	coating					1745:1751	electrodeposited HAP/CS/Gr/Gent coating	1713:1751	electrodeposited HAP/CS/Gr/Gent coating on Ti	1713:1757	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	4	50	theme	emission	647:654	arg1	microscopy					674:683	field emission scanning electron microscopy	641:683	field emission scanning electron microscopy	641:683	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	9	51	theme	tissue	1812:1817	arg1	engineering					1819:1829	bone tissue engineering	1807:1829	bone tissue engineering	1807:1829	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	6	52	theme	Large	985:989	arg1	area					1000:1003	Large specific area	985:1003	Large specific area of graphene	985:1015	Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix.					
32323414	4	53	theme	electron	665:672	arg1	microscopy					674:683	field emission scanning electron microscopy	641:683	field emission scanning electron microscopy	641:683	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	3	54	theme	graphene	512:519	arg1	influence					499:507	The influence	495:507	The influence of graphene	495:519	The influence of graphene was examined through detailed physicochemical and biological characterization.					
32323414	4	55	theme	infrared	618:625	arg1	spectroscopy					627:638	infrared spectroscopy	618:638	infrared spectroscopy	618:638	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	7	56	dep	reduction	1420:1428	arg1	units					1445:1449	>3 logarithmic units	1430:1449	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	8	57	theme	MTT	1521:1523	arg1	assay					1525:1529	MTT assay	1521:1529	MTT assay	1521:1529	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	3	58	theme	detailed	542:549	arg1	physicochemical					551:565	detailed physicochemical	542:565	detailed physicochemical	542:565	The influence of graphene was examined through detailed physicochemical and biological characterization.					
32323414	5	59	theme	strong	929:934	arg1	interaction					948:958	strong interfacial interaction	929:958	strong interfacial interaction between HAP, CS, and Gr	929:982	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	6	60	with	bonding	1032:1038	arg1	chitosan					1045:1052	chitosan	1045:1052	chitosan	1045:1052	Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix.					
32323414	4	61	theme	X-ray	714:718	arg1	diffraction					720:730	X-ray diffraction	714:730	X-ray diffraction	714:730	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	9	62	theme	prospective	1782:1792	arg1	material					1794:1801	a prospective material	1780:1801	a prospective material for bone tissue engineering	1780:1829	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	62	theme	prospective	1782:1792	arg1	coating					1745:1751	electrodeposited HAP/CS/Gr/Gent coating	1713:1751	electrodeposited HAP/CS/Gr/Gent coating on Ti	1713:1757	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	7	63	theme	agar	1270:1273	arg1	testing					1285:1291	agar diffusion testing	1270:1291	agar diffusion testing	1270:1291	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	6	64	theme	strong	1025:1030	arg1	bonding					1032:1038	strong bonding	1025:1038	strong bonding	1025:1038	Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix.					
32323414	7	65	theme	number	1413:1418	arg1	reduction					1420:1428	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	bactericidal, viable cells number reduction >3 logarithmic units	1386:1449	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	65	theme	number	1413:1418	arg1	aureus					1378:1383	Staphylococcus aureus	1363:1383	Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units)	1363:1450	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	0	66	theme	tissue	112:117	arg1	engineering					119:129	bone tissue engineering	107:129	bone tissue engineering	107:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	7	67	dep	coli	1476:1479	arg1	units					1513:1517	<3 logarithmic units	1498:1517	<3 logarithmic units	1498:1517	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	67	dep	coli	1476:1479	arg1	bacteriostatic					1482:1495	bacteriostatic	1482:1495	bacteriostatic	1482:1495	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	6	68	theme	graphene	1008:1015	arg1	area					1000:1003	Large specific area	985:1003	Large specific area of graphene	985:1015	Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix.					
32323414	2	69	theme	deposition	447:456	arg1	12 min					463:468	deposition time 12 min	447:468	deposition time 12 min	447:468	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	2	69	theme	deposition	447:456	arg1	5 V					442:444	5 V	442:444	5 V	442:444	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	8	70	theme	cell	1563:1566	arg1	viability					1568:1576	75% cell viability	1559:1576	75% cell viability	1559:1576	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	8	70	theme	cell	1563:1566	arg1	cytotoxicity					1545:1556	low cytotoxicity	1541:1556	low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines	1541:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	2	71	theme	titanium	479:486	arg1	foils					488:492	pure titanium foils	474:492	pure titanium foils	474:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	7	72	theme	antibacterial	1220:1232	arg1	kinetics					1243:1250	antibacterial activity kinetics	1220:1250	antibacterial activity kinetics in suspension and agar diffusion testing	1220:1291	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	1	73	theme	-reinforced	222:232	arg1	coating					244:250	graphene (Gr)-reinforced composite coating	209:250	graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent)	209:329	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	6	74	theme	polymer	1091:1097	arg1	matrix					1099:1104	the polymer matrix	1087:1104	the polymer matrix	1087:1104	Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix.					
32323414	9	75	theme	hard	1836:1839	arg1	implant					1848:1854	a hard tissue implant	1834:1854	a hard tissue implant	1834:1854	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	75	theme	hard	1836:1839	arg1	coating					1745:1751	electrodeposited HAP/CS/Gr/Gent coating	1713:1751	electrodeposited HAP/CS/Gr/Gent coating on Ti	1713:1757	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	8	76	theme	MRC-5	1587:1591	arg1	lines					1635:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	5	77	contain	had	873:875	arg1	coatings					864:871	Obtained coatings	855:871	Obtained coatings	855:871	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	5	77	contain	had	873:875	arg2	surfaces					908:915	porous, uniform, fracture-free surfaces	877:915	porous, uniform, fracture-free surfaces	877:915	Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr.					
32323414	0	78	theme	graphene-based	14:27	arg1	coating					53:59	Antibacterial graphene-based hydroxyapatite/chitosan coating	0:59	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering	0:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	8	79	theme	L929	1597:1600	arg1	lines					1635:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	2	80	theme	deposition	361:370	arg1	process					372:378	The deposition process	357:378	The deposition process	357:378	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	2	80	theme	deposition	361:370	arg1	step					406:409	a single step	397:409	a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils	397:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	1	81	theme	Electrophoretic	132:146	arg1	EPD					168:170	EPD	168:170	EPD	168:170	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	1	81	theme	Electrophoretic	132:146	arg1	process					159:165	Electrophoretic deposition process	132:165	Electrophoretic deposition process (EPD)	132:171	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	8	82	theme	70	1603:1604	arg1	%					1605:1605	%	1605:1605	%	1605:1605	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	4	83	theme	X-ray	744:748	arg1	analyses					764:771	X-ray photoelectron analyses	744:771	X-ray photoelectron analyses	744:771	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	2	84	theme	single	399:404	arg1	process					372:378	The deposition process	357:378	The deposition process	357:378	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	2	84	theme	single	399:404	arg1	step					406:409	a single step	397:409	a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils	397:492	The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils.					
32323414	7	85	theme	HAP/CS/Gr/Gent	1175:1188	arg1	coating					1190:1196	HAP/CS/Gr/Gent coating	1175:1196	HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units)	1175:1518	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	1	86	theme	composite	234:242	arg1	coating					244:250	graphene (Gr)-reinforced composite coating	209:250	graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent)	209:329	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	8	87	theme	cell	1607:1610	arg1	viability					1612:1620	70% cell viability	1603:1620	70% cell viability	1603:1620	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	4	88	theme	HAP/CS/Gr/Gent	824:837	arg1	coatings					839:846	HAP/CS/Gr/Gent coatings	824:846	HAP/CS/Gr/Gent coatings	824:846	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	0	89	with	coating	53:59	arg1	gentamicin					66:75	gentamicin	66:75	gentamicin	66:75	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	0	90	theme	potential	81:89	arg1	applications					91:102	potential applications	81:102	potential applications in bone tissue engineering	81:129	Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering.					
32323414	1	91	theme	antibiotic	302:311	arg1	Gent					325:328	Gent	325:328	Gent	325:328	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	1	91	theme	antibiotic	302:311	arg1	gentamicin					313:322	antibiotic gentamicin	302:322	antibiotic gentamicin (Gent)	302:329	Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension.					
32323414	7	92	theme	pronounced	1323:1332	arg1	effect					1348:1353	more pronounced antibacterial effect	1318:1353	more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units)	1318:1450	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	93	from	kinetics	1243:1250	arg1	testing					1285:1291	agar diffusion testing	1270:1291	agar diffusion testing	1270:1291	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	7	93	from	kinetics	1243:1250	arg1	suspension					1255:1264	suspension	1255:1264	suspension	1255:1264	Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units).					
32323414	8	94	theme	coating	1693:1699	arg1	biocompatibility					1658:1673	good biocompatibility	1653:1673	good biocompatibility of HAP/CS/Gr/Gent coating	1653:1699	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	8	95	theme	cell	1630:1633	arg1	lines					1635:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MRC-5 and L929 (70% cell viability) tested cell lines	1587:1639	MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.					
32323414	4	96	theme	HAP/CS/Gr	810:818	arg1	formation					787:795	the formation	783:795	the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti	783:852	Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti.					
32323414	9	97	theme	tissue	1841:1846	arg1	implant					1848:1854	a hard tissue implant	1834:1854	a hard tissue implant	1834:1854	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
32323414	9	97	theme	tissue	1841:1846	arg1	coating					1745:1751	electrodeposited HAP/CS/Gr/Gent coating	1713:1751	electrodeposited HAP/CS/Gr/Gent coating on Ti	1713:1757	Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.					
33141322	4	0	theme	 = 7.96	831:837	arg1	 + 0.22					850:856	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	8	1	theme	of the	1564:1569	arg1	percentages					1571:1581	prediction of the percentages	1553:1581	prediction of the percentages of D-Tyr in racemic Tyr mixtures	1553:1614	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	2	2	theme	modified	371:378	arg1	NSs/GCE					416:422	Mal-βCD/BP NSs/GCE	405:422	Mal-βCD/BP NSs/GCE	405:422	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	2	theme	modified	371:378	arg1	electrode					394:402	Mal-βCD modified glassy carbon electrode	363:402	Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE)	363:423	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	1	3	theme	tyrosine	294:301	arg1	enantiomers					309:319	tyrosine (Tyr) enantiomers	294:319	tyrosine (Tyr) enantiomers	294:319	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	7	4	theme	different	1353:1361	arg1	methods					1363:1369	different methods	1353:1369	different methods	1353:1369	The two similar sensors fabricated by different methods showed different recognition ability toward either Tyr or Trp enantiomers, and the underlying mechanism was discussed in detail.					
33141322	1	5	theme	Tyr	304:306	arg1	enantiomers					309:319	tyrosine (Tyr) enantiomers	294:319	tyrosine (Tyr) enantiomers	294:319	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	4	6	theme	Ip	824:825	arg1	 + 0.22					850:856	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	6	7	theme	BPNSs-G2-β-CD	1290:1302	arg1	composite					1304:1312	Nafion (NF)-stabilized BPNSs-G2-β-CD composite	1267:1312	Nafion (NF)-stabilized BPNSs-G2-β-CD composite	1267:1312	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	3	8	theme	chiral	726:731	arg1	recognition					733:743	effective chiral recognition	716:743	effective chiral recognition	716:743	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	7	9	theme	different	1378:1386	arg1	ability					1400:1406	different recognition ability	1378:1406	different recognition ability	1378:1406	The two similar sensors fabricated by different methods showed different recognition ability toward either Tyr or Trp enantiomers, and the underlying mechanism was discussed in detail.					
33141322	4	10	theme	μA	828:829	arg1	 + 0.22					850:856	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	7	11	theme	similar	1323:1329	arg1	sensors					1331:1337	The two similar sensors	1315:1337	The two similar sensors fabricated by different methods	1315:1369	The two similar sensors fabricated by different methods showed different recognition ability toward either Tyr or Trp enantiomers, and the underlying mechanism was discussed in detail.					
33141322	3	12	theme	hydroxyl	576:583	arg1	groups					585:590	the amino and hydroxyl groups	562:590	groups	585:590	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	12	theme	hydroxyl	576:583	arg1	Mal-βCD					649:655	Mal-βCD	649:655	Mal-βCD	649:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	12	theme	hydroxyl	576:583	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	0	13	theme	enantiomers	139:149	arg1	recognition					115:125	selective chiral recognition	98:125	selective chiral recognition of tyrosine enantiomers	98:149	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	3	14	theme	amino	566:570	arg1	groups					585:590	the amino and hydroxyl groups	562:590	groups	585:590	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	14	theme	amino	566:570	arg1	Mal-βCD					649:655	Mal-βCD	649:655	Mal-βCD	649:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	14	theme	amino	566:570	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	2	15	theme	drop-casting	459:470	arg1	method					472:477	a layer-to-layer drop-casting method	442:477	a layer-to-layer drop-casting method	442:477	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	9	16	theme	novel	1649:1653	arg1	approach					1655:1662	a novel approach	1647:1662	a novel approach for the fabrication of novel chiral platforms	1647:1708	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	8	17	from	percentages	1571:1581	arg1	mixtures					1607:1614	racemic Tyr mixtures	1595:1614	racemic Tyr mixtures	1595:1614	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	3	18	theme	chiral	623:628	arg1	Mal-βCD					649:655	Mal-βCD	649:655	Mal-βCD	649:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	18	theme	chiral	623:628	arg1	groups					639:644	the chiral hydroxyl groups	619:644	the chiral hydroxyl groups of Mal-βCD	619:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	18	theme	chiral	623:628	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	1	19	theme	novel	154:158	arg1	film					231:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	1	19	theme	novel	154:158	arg1	platform					175:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	0	20	theme	phosphorus	72:81	arg1	surface					55:61	the surface	51:61	the surface of black phosphorus	51:81	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	1	21	theme	sensing	167:173	arg1	film					231:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	1	21	theme	sensing	167:173	arg1	platform					175:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	9	22	theme	novel	1687:1691	arg1	platforms					1700:1708	novel chiral platforms	1687:1708	novel chiral platforms	1687:1708	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	6	23	theme	-stabilized	1278:1288	arg1	composite					1304:1312	Nafion (NF)-stabilized BPNSs-G2-β-CD composite	1267:1312	Nafion (NF)-stabilized BPNSs-G2-β-CD composite	1267:1312	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	9	24	theme	platforms	1700:1708	arg1	fabrication					1672:1682	the fabrication	1668:1682	the fabrication of novel chiral platforms	1668:1708	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	0	25	from	Immobilization	0:13	arg1	surface					55:61	the surface	51:61	the surface of black phosphorus	51:81	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	4	26	theme	μA	774:775	arg1	 + 0.28					797:803	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	0	27	theme	chiral	108:113	arg1	recognition					115:125	selective chiral recognition	98:125	selective chiral recognition of tyrosine enantiomers	98:149	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	4	28	theme	 = 11.40	777:784	arg1	 + 0.28					797:803	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	6	29	from	work	1099:1102	arg1	value					1061:1065	the value	1057:1065	the value of IL-Tyr/ID-Tyr (1.51) in this work	1057:1102	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	6	29	from	work	1099:1102	arg1	higher					1117:1122	higher	1117:1122	higher	1117:1122	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	2	30	theme	Mal-βCD/BP	405:414	arg1	NSs/GCE					416:422	Mal-βCD/BP NSs/GCE	405:422	Mal-βCD/BP NSs/GCE	405:422	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	30	theme	Mal-βCD/BP	405:414	arg1	electrode					394:402	Mal-βCD modified glassy carbon electrode	363:402	Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE)	363:423	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	4	31	from	equations	757:765	arg1	range					894:898	the concentration range 0.01-1.00 mM	876:911	the concentration range 0.01-1.00 mM	876:911	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	9	32	theme	L-	1740:1741	arg1	detection					1727:1735	the practical detection	1713:1735	the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract	1713:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	4	33	theme	Ip	770:771	arg1	 + 0.28					797:803	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	0	34	theme	6-O-α-maltosyl-β-cyclodextrin	18:46	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus	0:81	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	9	35	theme	D-enantiomer	1746:1757	arg1	detection					1727:1735	the practical detection	1713:1735	the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract	1713:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	3	36	theme	Mal-βCD	649:655	arg1	groups					585:590	the amino and hydroxyl groups	562:590	groups	585:590	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	36	theme	Mal-βCD	649:655	arg1	Mal-βCD					649:655	Mal-βCD	649:655	Mal-βCD	649:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	36	theme	Mal-βCD	649:655	arg1	groups					639:644	the chiral hydroxyl groups	619:644	the chiral hydroxyl groups of Mal-βCD	619:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	36	theme	Mal-βCD	649:655	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	5	37	theme	 for L-Tyr	964:973	arg1	The limits					933:942	The limits	933:942	The limits of detection (S/N=3) for L-Tyr and D-Tyr were	933:988	The limits of detection (S/N=3) for L-Tyr and D-Tyr were 4.81 and 6.89 µM, respectively.					
33141322	9	38	theme	racemic	1762:1768	arg1	abstract					1793:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	6	39	from	value	1061:1065	arg1	work					1099:1102	this work	1094:1102	this work	1094:1102	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	2	40	theme	Mal-βCD	363:369	arg1	NSs/GCE					416:422	Mal-βCD/BP NSs/GCE	405:422	Mal-βCD/BP NSs/GCE	405:422	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	40	theme	Mal-βCD	363:369	arg1	electrode					394:402	Mal-βCD modified glassy carbon electrode	363:402	Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE)	363:423	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	5	41	theme	D-Tyr were	979:988	arg1	The limits					933:942	The limits	933:942	The limits of detection (S/N=3) for L-Tyr and D-Tyr were	933:988	The limits of detection (S/N=3) for L-Tyr and D-Tyr were 4.81 and 6.89 µM, respectively.					
33141322	6	42	theme	IL-Tyr/ID-Tyr	1070:1082	arg1	value					1061:1065	the value	1057:1065	the value of IL-Tyr/ID-Tyr (1.51) in this work	1057:1102	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	6	42	theme	IL-Tyr/ID-Tyr	1070:1082	arg1	higher					1117:1122	higher	1117:1122	higher	1117:1122	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	9	43	theme	mixtures.Graphical	1774:1791	arg1	abstract					1793:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	3	44	theme	hydrogen	689:696	arg1	bonds					698:702	intermolecular hydrogen bonds	674:702	intermolecular hydrogen bonds	674:702	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	6	45	from	IL-Tyr/ID-Tyr	1070:1082	arg1	work					1099:1102	this work	1094:1102	this work	1094:1102	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	8	46	theme	D-Tyr	1586:1590	arg1	percentages					1571:1581	prediction of the percentages	1553:1581	prediction of the percentages of D-Tyr in racemic Tyr mixtures	1553:1614	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	7	47	theme	Trp	1429:1431	arg1	enantiomers					1433:1443	Trp enantiomers	1429:1443	Trp enantiomers	1429:1443	The two similar sensors fabricated by different methods showed different recognition ability toward either Tyr or Trp enantiomers, and the underlying mechanism was discussed in detail.					
33141322	5	48	theme	detection	947:955	arg1	 for L-Tyr					964:973	detection (S/N=3) for L-Tyr	947:973	detection (S/N=3) for L-Tyr	947:973	The limits of detection (S/N=3) for L-Tyr and D-Tyr were 4.81 and 6.89 µM, respectively.					
33141322	8	49	theme	proposed	1522:1529	arg1	sensor					1538:1543	the proposed chiral sensor	1518:1543	the proposed chiral sensor	1518:1543	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	8	50	theme	racemic	1595:1601	arg1	mixtures					1607:1614	racemic Tyr mixtures	1595:1614	racemic Tyr mixtures	1595:1614	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	1	51	theme	electrochemical	263:277	arg1	recognition					279:289	selective electrochemical recognition	253:289	selective electrochemical recognition of tyrosine (Tyr) enantiomers	253:319	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	5	52	theme	S/N=3	958:962	arg1	 for L-Tyr					964:973	detection (S/N=3) for L-Tyr	947:973	detection (S/N=3) for L-Tyr	947:973	The limits of detection (S/N=3) for L-Tyr and D-Tyr were 4.81 and 6.89 µM, respectively.					
33141322	3	53	theme	effective	716:724	arg1	recognition					733:743	effective chiral recognition	716:743	effective chiral recognition	716:743	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	6	54	theme	tryptophan	1201:1210	arg1	enantiomers					1218:1228	tryptophan (Trp) enantiomers	1201:1228	tryptophan (Trp) enantiomers	1201:1228	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	2	55	theme	glassy	380:385	arg1	NSs/GCE					416:422	Mal-βCD/BP NSs/GCE	405:422	Mal-βCD/BP NSs/GCE	405:422	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	55	theme	glassy	380:385	arg1	electrode					394:402	Mal-βCD modified glassy carbon electrode	363:402	Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE)	363:423	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	8	56	theme	prediction	1553:1562	arg1	percentages					1571:1581	prediction of the percentages	1553:1581	prediction of the percentages of D-Tyr in racemic Tyr mixtures	1553:1614	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	2	57	theme	Black	322:326	arg1	nanosheets					339:348	Black phosphorus nanosheets	322:348	Black phosphorus nanosheets (BP NSs)	322:357	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	57	theme	Black	322:326	arg1	NSs					354:356	BP NSs	351:356	BP NSs	351:356	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	7	58	theme	recognition	1388:1398	arg1	ability					1400:1406	different recognition ability	1378:1406	different recognition ability	1378:1406	The two similar sensors fabricated by different methods showed different recognition ability toward either Tyr or Trp enantiomers, and the underlying mechanism was discussed in detail.					
33141322	4	59	theme	CD-Tyr	839:844	arg1	 + 0.22					850:856	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	6	60	theme	previous	1179:1186	arg1	study					1188:1192	our previous study	1175:1192	our previous study	1175:1192	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	9	61	from	detection	1727:1735	arg1	abstract					1793:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	2	62	theme	BP	351:352	arg1	nanosheets					339:348	Black phosphorus nanosheets	322:348	Black phosphorus nanosheets (BP NSs)	322:357	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	62	theme	BP	351:352	arg1	NSs					354:356	BP NSs	351:356	BP NSs	351:356	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	9	63	theme	chiral	1621:1626	arg1	sensor					1628:1633	The chiral sensor	1617:1633	The chiral sensor	1617:1633	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	1	64	theme	-based	224:229	arg1	film					231:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	1	64	theme	-based	224:229	arg1	platform					175:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	4	65	theme	mM	847:848	arg1	 + 0.22					850:856	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583)	824:871	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	6	66	theme	IL-Trp/ID-Trp	1142:1154	arg1	value					1133:1137	the value	1129:1137	the value of IL-Trp/ID-Trp (1.49) reported in our previous study	1129:1192	An interesting phenomenon was that the value of IL-Tyr/ID-Tyr (1.51) in this work was slightly higher than the value of IL-Trp/ID-Trp (1.49) reported in our previous study, where tryptophan (Trp) enantiomers were electrochemically recognized by Nafion (NF)-stabilized BPNSs-G2-β-CD composite.					
33141322	4	67	theme	 + 0.22	850:856	arg1	equations					757:765	Two linear equations	746:765	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM	746:911	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	3	68	theme	enantiomers	603:613	arg1	groups					585:590	the amino and hydroxyl groups	562:590	groups	585:590	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	68	theme	enantiomers	603:613	arg1	Mal-βCD					649:655	Mal-βCD	649:655	Mal-βCD	649:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	68	theme	enantiomers	603:613	arg1	groups					639:644	the chiral hydroxyl groups	619:644	the chiral hydroxyl groups of Mal-βCD	619:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	68	theme	enantiomers	603:613	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	1	69	theme	chiral	160:165	arg1	film					231:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film	185:234	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	1	69	theme	chiral	160:165	arg1	platform					175:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform	152:182	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	0	70	theme	selective	98:106	arg1	recognition					115:125	selective chiral recognition	98:125	selective chiral recognition of tyrosine enantiomers	98:149	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	9	71	theme	chiral	1693:1698	arg1	platforms					1700:1708	novel chiral platforms	1687:1708	novel chiral platforms	1687:1708	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	2	72	theme	phosphorus	328:337	arg1	nanosheets					339:348	Black phosphorus nanosheets	322:348	Black phosphorus nanosheets (BP NSs)	322:357	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	72	theme	phosphorus	328:337	arg1	NSs					354:356	BP NSs	351:356	BP NSs	351:356	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	4	73	theme	concentration	880:892	arg1	range					894:898	the concentration range 0.01-1.00 mM	876:911	the concentration range 0.01-1.00 mM	876:911	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	0	74	theme	tyrosine	130:137	arg1	enantiomers					139:149	tyrosine enantiomers	130:149	tyrosine enantiomers	130:149	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	9	75	theme	practical	1717:1725	arg1	detection					1727:1735	the practical detection	1713:1735	the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract	1713:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	4	76	theme	linear	750:755	arg1	equations					757:765	Two linear equations	746:765	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM	746:911	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	1	77	theme	enantiomers	309:319	arg1	recognition					279:289	selective electrochemical recognition	253:289	selective electrochemical recognition of tyrosine (Tyr) enantiomers	253:319	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	2	78	theme	layer-to-layer	444:457	arg1	method					472:477	a layer-to-layer drop-casting method	442:477	a layer-to-layer drop-casting method	442:477	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	3	79	theme	hydroxyl	630:637	arg1	Mal-βCD					649:655	Mal-βCD	649:655	Mal-βCD	649:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	79	theme	hydroxyl	630:637	arg1	groups					639:644	the chiral hydroxyl groups	619:644	the chiral hydroxyl groups of Mal-βCD	619:655	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	79	theme	hydroxyl	630:637	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	4	80	theme	 + 0.28	797:803	arg1	equations					757:765	Two linear equations	746:765	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM	746:911	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	0	81	theme	black	66:70	arg1	phosphorus					72:81	black phosphorus	66:81	black phosphorus	66:81	Immobilization of 6-O-α-maltosyl-β-cyclodextrin on the surface of black phosphorus nanosheets for selective chiral recognition of tyrosine enantiomers.					
33141322	7	82	theme	underlying	1454:1463	arg1	mechanism					1465:1473	the underlying mechanism	1450:1473	the underlying mechanism	1450:1473	The two similar sensors fabricated by different methods showed different recognition ability toward either Tyr or Trp enantiomers, and the underlying mechanism was discussed in detail.					
33141322	9	83	theme	Tyr	1770:1772	arg1	abstract					1793:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	racemic Tyr mixtures.Graphical abstract	1762:1800	The chiral sensor may provide a novel approach for the fabrication of novel chiral platforms in the practical detection of L- or D-enantiomer in racemic Tyr mixtures.Graphical abstract.					
33141322	3	84	theme	intermolecular	674:687	arg1	bonds					698:702	intermolecular hydrogen bonds	674:702	intermolecular hydrogen bonds	674:702	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	3	85	theme	Tyr	599:601	arg1	enantiomers					603:613	the Tyr enantiomers	595:613	the Tyr enantiomers	595:613	It is proposed that the amino and hydroxyl groups of the Tyr enantiomers and the chiral hydroxyl groups of Mal-βCD selectively form intermolecular hydrogen bonds to dominate effective chiral recognition.					
33141322	8	86	theme	chiral	1531:1536	arg1	sensor					1538:1543	the proposed chiral sensor	1518:1543	the proposed chiral sensor	1518:1543	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	4	87	theme	CL-Tyr	786:791	arg1	 + 0.28					797:803	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	8	88	theme	Tyr	1603:1605	arg1	mixtures					1607:1614	racemic Tyr mixtures	1595:1614	racemic Tyr mixtures	1595:1614	More importantly, the proposed chiral sensor enables prediction of the percentages of D-Tyr in racemic Tyr mixtures.					
33141322	1	89	theme	selective	253:261	arg1	recognition					279:289	selective electrochemical recognition	253:289	selective electrochemical recognition of tyrosine (Tyr) enantiomers	253:319	A novel chiral sensing platform, 6-O-α-maltosyl-β-cyclodextrin (Mal-βCD)-based film, is proposed for selective electrochemical recognition of tyrosine (Tyr) enantiomers.					
33141322	4	90	theme	mM	794:795	arg1	 + 0.28					797:803	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147)	770:818	Two linear equations of Ip (μA) = 11.40 CL-Tyr (mM) + 0.28 (R2 = 0.99147) and Ip (μA) = 7.96 CD-Tyr (mM) + 0.22 (R2 = 0.99583) in the concentration range 0.01-1.00 mM have been obtained.					
33141322	2	91	theme	carbon	387:392	arg1	NSs/GCE					416:422	Mal-βCD/BP NSs/GCE	405:422	Mal-βCD/BP NSs/GCE	405:422	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
33141322	2	91	theme	carbon	387:392	arg1	electrode					394:402	Mal-βCD modified glassy carbon electrode	363:402	Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE)	363:423	Black phosphorus nanosheets (BP NSs) and Mal-βCD modified glassy carbon electrode (Mal-βCD/BP NSs/GCE) were prepared by a layer-to-layer drop-casting method, and the platform was easy to fabricate and facile to operate.					
34560486	10	0	theme	competitive	1475:1485	arg1	system					1487:1492	the binary competitive system	1464:1492	the binary competitive system	1464:1492	Compared with the binary competitive system, the material exhibited a specific selectivity to the adsorption of Cu (II).					
34560486	7	1	theme	experimental	1081:1092	arg1	conditions					1094:1103	suitable experimental conditions	1072:1103	suitable experimental conditions	1072:1103	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	13	2	theme	Cd	1884:1885	arg1	ions					1892:1895	Cu (II) and Cd (II) ions	1872:1895	Cu (II) and Cd (II) ions	1872:1895	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	2	3	theme	nano-calcium	309:320	arg1	hydrogel					347:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	2	3	theme	nano-calcium	309:320	arg1	adsorbent					388:396	adsorbent	388:396	adsorbent	388:396	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	1	4	theme	ions	232:235	arg1	adsorption					210:219	the adsorption	206:219	the adsorption of various ions and their utilisation	206:257	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	8	5	theme	adsorption	1205:1214	arg1	kinetics					1216:1223	The time-dependent adsorption kinetics	1186:1223	The time-dependent adsorption kinetics	1186:1223	The time-dependent adsorption kinetics followed a quasi-second order model, and the adsorption process followed the Langmuir model.					
34560486	15	6	theme	excellent	2164:2172	arg1	stability					2174:2182	excellent stability	2164:2182	excellent stability	2164:2182	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	3	7	theme	Brunauer-Emmett-Teller	461:482	arg1	results					484:490	Brunauer-Emmett-Teller results	461:490	Brunauer-Emmett-Teller results	461:490	Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO3/chitin hydrogel exhibited loose macropores and mesopores.					
34560486	3	8	theme	CaCO3/chitin	522:533	arg1	hydrogel					535:542	the synthesised CaCO3/chitin hydrogel	506:542	the synthesised CaCO3/chitin hydrogel	506:542	Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO3/chitin hydrogel exhibited loose macropores and mesopores.					
34560486	1	9	theme	functional	159:168	arg1	groups					170:175	limited functional groups	151:175	limited functional groups	151:175	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	8	10	theme	quasi-second	1236:1247	arg1	model					1255:1259	a quasi-second order model	1234:1259	a quasi-second order model	1234:1259	The time-dependent adsorption kinetics followed a quasi-second order model, and the adsorption process followed the Langmuir model.					
34560486	0	11	theme	nano-calcium	65:76	arg1	hydrogels					110:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	3	12	theme	Scanning	428:435	arg1	microscopy					446:455	Scanning electron microscopy	428:455	Scanning electron microscopy	428:455	Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO3/chitin hydrogel exhibited loose macropores and mesopores.					
34560486	5	13	theme	unmodified	831:840	arg1	hydrogel					849:856	the unmodified chitin hydrogel	827:856	the unmodified chitin hydrogel	827:856	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	0	14	theme	high-intensity	88:101	arg1	hydrogels					110:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	15	15	theme	Cd	2016:2017	arg1	higher					2093:2098	higher	2093:2098	higher	2093:2098	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	15	15	theme	Cd	2016:2017	arg1	efficiencies					2035:2046	The Cu (II) and Cd (II)ion removal efficiencies	2000:2046	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles	2000:2086	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	5	16	theme	Cd	899:900	arg1	ions					907:910	Cu (II) and Cd (II) ions	887:910	Cu (II) and Cd (II) ions	887:910	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	8	17	theme	adsorption	1270:1279	arg1	process					1281:1287	the adsorption process	1266:1287	the adsorption process	1266:1287	The time-dependent adsorption kinetics followed a quasi-second order model, and the adsorption process followed the Langmuir model.					
34560486	6	18	theme	dosage	973:978	arg1	effect					926:931	The effect	922:931	The effect of pH, initial concentration, adsorbent dosage, and temperature	922:995	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	13	19	theme	Cu	1872:1873	arg1	ions					1892:1895	Cu (II) and Cd (II) ions	1872:1895	Cu (II) and Cd (II) ions	1872:1895	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	15	20	theme	adsorption	2070:2079	arg1	cycles					2081:2086	five successive adsorption cycles	2054:2086	five successive adsorption cycles	2054:2086	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	8	21	theme	Langmuir	1302:1309	arg1	model					1311:1315	the Langmuir model	1298:1315	the Langmuir model	1298:1315	The time-dependent adsorption kinetics followed a quasi-second order model, and the adsorption process followed the Langmuir model.					
34560486	1	22	theme	natural	126:132	arg1	polymers					134:141	Most natural polymers	121:141	Most natural polymers	121:141	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	6	23	theme	initial	940:946	arg1	concentration					948:960	initial concentration	940:960	initial concentration	940:960	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	13	24	theme	excellent	1840:1848	arg1	effects					1861:1867	excellent adsorption effects	1840:1867	excellent adsorption effects on Cu (II) and Cd (II) ions	1840:1895	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	11	25	theme	metal	1686:1690	arg1	ions					1692:1695	the metal ions	1682:1695	the metal ions	1682:1695	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34560486	6	26	theme	pH	936:937	arg1	effect					926:931	The effect	922:931	The effect of pH, initial concentration, adsorbent dosage, and temperature	922:995	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	11	27	theme	photoelectron	1577:1589	arg1	XPS					1605:1607	XPS	1605:1607	XPS	1605:1607	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34560486	11	27	theme	photoelectron	1577:1589	arg1	spectroscopy					1591:1602	X-ray photoelectron spectroscopy	1571:1602	X-ray photoelectron spectroscopy (XPS)	1571:1608	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34560486	7	28	theme	hydrogel	1154:1161	arg1	rate					1129:1132	the maximum adsorption rate	1106:1132	the maximum adsorption rate of the CaCO3/chitin hydrogel	1106:1161	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	7	28	theme	hydrogel	1154:1161	arg1	%					1183:1183	approximately 96%	1167:1183	approximately 96%	1167:1183	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	2	29	theme	chitin	340:345	arg1	hydrogel					347:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	2	29	theme	chitin	340:345	arg1	adsorbent					388:396	adsorbent	388:396	adsorbent	388:396	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	0	30	theme	copper	14:19	arg1	ions					43:46	copper (II) and cadmium (II) ions	14:46	copper (II) and cadmium (II) ions	14:46	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	1	31	theme	utilisation	247:257	arg1	adsorption					210:219	the adsorption	206:219	the adsorption of various ions and their utilisation	206:257	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	13	32	from	effects	1861:1867	arg1	ions					1892:1895	Cu (II) and Cd (II) ions	1872:1895	Cu (II) and Cd (II) ions	1872:1895	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	15	33	theme	successive	2059:2068	arg1	cycles					2081:2086	five successive adsorption cycles	2054:2086	five successive adsorption cycles	2054:2086	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	2	34	theme	Cd	414:415	arg1	ions					422:425	Cu (II) and Cd (II) ions	402:425	Cu (II) and Cd (II) ions	402:425	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	7	35	theme	maximum	1110:1116	arg1	rate					1129:1132	the maximum adsorption rate	1106:1132	the maximum adsorption rate of the CaCO3/chitin hydrogel	1106:1161	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	7	35	theme	maximum	1110:1116	arg1	%					1183:1183	approximately 96%	1167:1183	approximately 96%	1167:1183	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	9	36	theme	adsorption	1330:1339	arg1	capacities					1341:1350	The maximum adsorption capacities	1318:1350	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve	1318:1405	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	9	36	theme	adsorption	1330:1339	arg1	194.61					1412:1417	194.61	1412:1417	194.61	1412:1417	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	5	37	theme	CaCO3/chitin	779:790	arg1	hydrogel					792:799	CaCO3/chitin hydrogel	779:799	CaCO3/chitin hydrogel	779:799	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	4	38	dep	transform	608:616	arg1	infrared					618:625	infrared	618:625	transform infrared	608:625	Subsequently, Fourier transform infrared, Raman, and X-ray diffraction characterisation characterisation proved that chitin was successfully doped with nano-CaCO3.					
34560486	10	39	theme	binary	1468:1473	arg1	system					1487:1492	the binary competitive system	1464:1492	the binary competitive system	1464:1492	Compared with the binary competitive system, the material exhibited a specific selectivity to the adsorption of Cu (II).					
34560486	13	40	theme	water	1915:1919	arg1	sample					1921:1926	a water sample	1913:1926	a water sample	1913:1926	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	2	41	theme	in-situ-doped	295:307	arg1	hydrogel					347:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	2	41	theme	in-situ-doped	295:307	arg1	adsorbent					388:396	adsorbent	388:396	adsorbent	388:396	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	12	42	theme	active	1774:1779	arg1	sites					1792:1796	more active adsorption sites	1769:1796	more active adsorption sites	1769:1796	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	12	43	theme	more	1769:1772	arg1	sites					1792:1796	more active adsorption sites	1769:1796	more active adsorption sites	1769:1796	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	15	44	theme	ion	2023:2025	arg1	higher					2093:2098	higher	2093:2098	higher	2093:2098	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	15	44	theme	ion	2023:2025	arg1	efficiencies					2035:2046	The Cu (II) and Cd (II)ion removal efficiencies	2000:2046	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles	2000:2086	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	3	45	theme	electron	437:444	arg1	microscopy					446:455	Scanning electron microscopy	428:455	Scanning electron microscopy	428:455	Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO3/chitin hydrogel exhibited loose macropores and mesopores.					
34560486	5	46	theme	hydrogel	792:799	arg1	superior					806:813	superior	806:813	superior	806:813	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	5	46	theme	hydrogel	792:799	arg1	properties					765:774	The mechanical properties	750:774	The mechanical properties of CaCO3/chitin hydrogel	750:799	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	12	47	theme	calcium	1728:1734	arg1	carbonate					1736:1744	calcium carbonate	1728:1744	calcium carbonate	1728:1744	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	5	48	theme	mechanical	754:763	arg1	superior					806:813	superior	806:813	superior	806:813	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	5	48	theme	mechanical	754:763	arg1	properties					765:774	The mechanical properties	750:774	The mechanical properties of CaCO3/chitin hydrogel	750:799	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	7	49	theme	suitable	1072:1079	arg1	conditions					1094:1103	suitable experimental conditions	1072:1103	suitable experimental conditions	1072:1103	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	2	50	theme	carbonate	322:330	arg1	hydrogel					347:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	2	50	theme	carbonate	322:330	arg1	adsorbent					388:396	adsorbent	388:396	adsorbent	388:396	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	14	51	theme	novel	1939:1943	arg1	material					1945:1952	the novel material	1935:1952	the novel material	1935:1952	Thus, the novel material exhibits excellent potential for application.					
34560486	9	52	theme	Cd	1367:1368	arg1	capacities					1341:1350	The maximum adsorption capacities	1318:1350	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve	1318:1405	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	9	52	theme	Cd	1367:1368	arg1	194.61					1412:1417	194.61	1412:1417	194.61	1412:1417	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	10	53	theme	specific	1520:1527	arg1	selectivity					1529:1539	a specific selectivity	1518:1539	a specific selectivity to the adsorption of Cu (II)	1518:1568	Compared with the binary competitive system, the material exhibited a specific selectivity to the adsorption of Cu (II).					
34560486	15	54	theme	Cu	2004:2005	arg1	higher					2093:2098	higher	2093:2098	higher	2093:2098	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	15	54	theme	Cu	2004:2005	arg1	efficiencies					2035:2046	The Cu (II) and Cd (II)ion removal efficiencies	2000:2046	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles	2000:2086	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	1	55	theme	various	224:230	arg1	ions					232:235	various ions	224:235	various ions	224:235	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	15	56	theme	removal	2027:2033	arg1	higher					2093:2098	higher	2093:2098	higher	2093:2098	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	15	56	theme	removal	2027:2033	arg1	efficiencies					2035:2046	The Cu (II) and Cd (II)ion removal efficiencies	2000:2046	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles	2000:2086	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	8	57	theme	time-dependent	1190:1203	arg1	kinetics					1216:1223	The time-dependent adsorption kinetics	1186:1223	The time-dependent adsorption kinetics	1186:1223	The time-dependent adsorption kinetics followed a quasi-second order model, and the adsorption process followed the Langmuir model.					
34560486	3	58	theme	synthesised	510:520	arg1	hydrogel					535:542	the synthesised CaCO3/chitin hydrogel	506:542	the synthesised CaCO3/chitin hydrogel	506:542	Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO3/chitin hydrogel exhibited loose macropores and mesopores.					
34560486	1	59	theme	limited	151:157	arg1	groups					170:175	limited functional groups	151:175	limited functional groups	151:175	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	0	60	theme	carbonate	78:86	arg1	hydrogels					110:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	0	61	theme	chitin	103:108	arg1	hydrogels					110:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	3	62	theme	loose	554:558	arg1	macropores					560:569	loose macropores	554:569	loose macropores	554:569	Scanning electron microscopy and Brunauer-Emmett-Teller results revealed that the synthesised CaCO3/chitin hydrogel exhibited loose macropores and mesopores.					
34560486	9	63	theme	Langmuir	1392:1399	arg1	curve					1401:1405	the Langmuir curve	1388:1405	the Langmuir curve	1388:1405	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	5	64	theme	chitin	842:847	arg1	hydrogel					849:856	the unmodified chitin hydrogel	827:856	the unmodified chitin hydrogel	827:856	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	8	65	theme	order	1249:1253	arg1	model					1255:1259	a quasi-second order model	1234:1259	a quasi-second order model	1234:1259	The time-dependent adsorption kinetics followed a quasi-second order model, and the adsorption process followed the Langmuir model.					
34560486	10	66	theme	Cu	1562:1563	arg1	adsorption					1548:1557	the adsorption	1544:1557	the adsorption of Cu (II)	1544:1568	Compared with the binary competitive system, the material exhibited a specific selectivity to the adsorption of Cu (II).					
34560486	13	67	theme	adsorption	1850:1859	arg1	effects					1861:1867	excellent adsorption effects	1840:1867	excellent adsorption effects on Cu (II) and Cd (II) ions	1840:1895	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	11	68	with	chelation	1667:1675	arg1	ions					1692:1695	the metal ions	1682:1695	the metal ions	1682:1695	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34560486	5	69	theme	Cu	887:888	arg1	ions					907:910	Cu (II) and Cd (II) ions	887:910	Cu (II) and Cd (II) ions	887:910	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	6	70	theme	adsorbent	963:971	arg1	dosage					973:978	adsorbent dosage	963:978	adsorbent dosage	963:978	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	4	71	theme	X-ray	639:643	arg1	diffraction					645:655	X-ray diffraction	639:655	X-ray diffraction	639:655	Subsequently, Fourier transform infrared, Raman, and X-ray diffraction characterisation characterisation proved that chitin was successfully doped with nano-CaCO3.					
34560486	13	72	theme	novel	1815:1819	arg1	material					1821:1828	The novel material	1811:1828	The novel material	1811:1828	The novel material exhibited excellent adsorption effects on Cu (II) and Cd (II) ions when applied to a water sample.					
34560486	0	73	theme	ions	43:46	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.	0:119	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	1	74	theme	Most	121:124	arg1	polymers					134:141	Most natural polymers	121:141	Most natural polymers	121:141	Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation.					
34560486	6	75	theme	concentration	948:960	arg1	effect					926:931	The effect	922:931	The effect of pH, initial concentration, adsorbent dosage, and temperature	922:995	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	4	76	theme	characterisation	657:672	arg1	characterisation					674:689	characterisation characterisation	657:689	characterisation characterisation	657:689	Subsequently, Fourier transform infrared, Raman, and X-ray diffraction characterisation characterisation proved that chitin was successfully doped with nano-CaCO3.					
34560486	0	77	theme	in	51:52	arg1	hydrogels					110:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	0	78	theme	doped	59:63	arg1	hydrogels					110:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	in situ doped nano-calcium carbonate high-intensity chitin hydrogels	51:118	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	0	79	dep	in	51:52	arg1	situ					54:57	situ	54:57	situ	54:57	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	6	80	theme	adsorption	1027:1036	arg1	properties					1038:1047	the adsorption properties	1023:1047	the adsorption properties of the hydrogel	1023:1063	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	7	81	theme	CaCO3/chitin	1141:1152	arg1	hydrogel					1154:1161	the CaCO3/chitin hydrogel	1137:1161	the CaCO3/chitin hydrogel	1137:1161	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	2	82	theme	Cu	402:403	arg1	ions					422:425	Cu (II) and Cd (II) ions	402:425	Cu (II) and Cd (II) ions	402:425	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	7	83	theme	adsorption	1118:1127	arg1	rate					1129:1132	the maximum adsorption rate	1106:1132	the maximum adsorption rate of the CaCO3/chitin hydrogel	1106:1161	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	7	83	theme	adsorption	1118:1127	arg1	%					1183:1183	approximately 96%	1167:1183	approximately 96%	1167:1183	Under suitable experimental conditions, the maximum adsorption rate of the CaCO3/chitin hydrogel was approximately 96%.					
34560486	15	84	theme	composite	2135:2143	arg1	material					2145:2152	the composite material	2131:2152	the composite material	2131:2152	The Cu (II) and Cd (II)ion removal efficiencies after five successive adsorption cycles were higher than 90%, which indicated that the composite material exhibited excellent stability and reproducibility.					
34560486	14	85	theme	excellent	1963:1971	arg1	potential					1973:1981	excellent potential	1963:1981	excellent potential for application	1963:1997	Thus, the novel material exhibits excellent potential for application.					
34560486	12	86	dep	nanoparticles	1746:1758	arg1	provided					1760:1767	provided	1760:1767	nanoparticles provided more active adsorption sites for the gel	1746:1808	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	2	87	theme	CaCO3	333:337	arg1	hydrogel					347:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	2	87	theme	CaCO3	333:337	arg1	adsorbent					388:396	adsorbent	388:396	adsorbent	388:396	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	9	88	theme	maximum	1322:1328	arg1	capacities					1341:1350	The maximum adsorption capacities	1318:1350	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve	1318:1405	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	9	88	theme	maximum	1322:1328	arg1	194.61					1412:1417	194.61	1412:1417	194.61	1412:1417	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	6	89	theme	temperature	985:995	arg1	effect					926:931	The effect	922:931	The effect of pH, initial concentration, adsorbent dosage, and temperature	922:995	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	5	90	dep	adsorb	880:885	arg1	in					912:913	in	912:913	in	912:913	The mechanical properties of CaCO3/chitin hydrogel were superior to those of the unmodified chitin hydrogel and could efficiently adsorb Cu (II) and Cd (II) ions in water.					
34560486	0	91	theme	cadmium	30:36	arg1	ions					43:46	copper (II) and cadmium (II) ions	14:46	copper (II) and cadmium (II) ions	14:46	Adsorption of copper (II) and cadmium (II) ions by in situ doped nano-calcium carbonate high-intensity chitin hydrogels.					
34560486	12	92	theme	adsorption	1781:1790	arg1	sites					1792:1796	more active adsorption sites	1769:1796	more active adsorption sites	1769:1796	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	4	93	dep	Fourier	600:606	arg1	transform					608:616	transform	608:616	transform infrared	608:625	Subsequently, Fourier transform infrared, Raman, and X-ray diffraction characterisation characterisation proved that chitin was successfully doped with nano-CaCO3.					
34560486	4	93	dep	Fourier	600:606	arg1	characterisation					674:689	characterisation characterisation	657:689	characterisation characterisation	657:689	Subsequently, Fourier transform infrared, Raman, and X-ray diffraction characterisation characterisation proved that chitin was successfully doped with nano-CaCO3.					
34560486	12	94	theme	carbonate	1736:1744	arg1	compounding					1713:1723	The successful compounding	1698:1723	The successful compounding of calcium carbonate	1698:1744	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	2	95	theme	novel	289:293	arg1	hydrogel					347:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel	287:354	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	2	95	theme	novel	289:293	arg1	adsorbent					388:396	adsorbent	388:396	adsorbent	388:396	To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO3) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions.					
34560486	9	96	theme	Cu	1355:1356	arg1	capacities					1341:1350	The maximum adsorption capacities	1318:1350	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve	1318:1405	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	9	96	theme	Cu	1355:1356	arg1	194.61					1412:1417	194.61	1412:1417	194.61	1412:1417	The maximum adsorption capacities of Cu (II) and Cd (II) according to the Langmuir curve were 194.61 and 191.58 mg/g, respectively.					
34560486	11	97	theme	oxygen	1637:1642	arg1	atoms					1644:1648	oxygen atoms	1637:1648	oxygen atoms	1637:1648	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34560486	12	98	theme	successful	1702:1711	arg1	compounding					1713:1723	The successful compounding	1698:1723	The successful compounding of calcium carbonate	1698:1744	The successful compounding of calcium carbonate nanoparticles provided more active adsorption sites for the gel.					
34560486	6	99	theme	hydrogel	1056:1063	arg1	properties					1038:1047	the adsorption properties	1023:1047	the adsorption properties of the hydrogel	1023:1063	The effect of pH, initial concentration, adsorbent dosage, and temperature was assessed to determine the adsorption properties of the hydrogel.					
34560486	11	100	theme	X-ray	1571:1575	arg1	XPS					1605:1607	XPS	1605:1607	XPS	1605:1607	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34560486	11	100	theme	X-ray	1571:1575	arg1	spectroscopy					1591:1602	X-ray photoelectron spectroscopy	1571:1602	X-ray photoelectron spectroscopy (XPS)	1571:1608	X-ray photoelectron spectroscopy (XPS) revealed that nitrogen and oxygen atoms were involved in chelation with the metal ions.					
34794733	7	0	theme	model	1078:1082	arg1	group					1084:1088	the model group	1074:1088	the model group	1074:1088	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	11	1	theme	observation	1730:1740	arg1	P < 0.05					1750:1757	P < 0.05	1750:1757	P < 0.05	1750:1757	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	1	theme	observation	1730:1740	arg1	period					1742:1747	the observation period	1726:1747	the observation period (P < 0.05)	1726:1758	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	8	2	from	rates	1133:1137	arg1	group					1192:1196	the combination therapy group	1168:1196	the combination therapy group	1168:1196	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	8	2	from	rates	1133:1137	arg1	group					1158:1162	the gel therapy group	1142:1162	the gel therapy group	1142:1162	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	1	3	theme	intrauterine	270:281	arg1	treatment					298:306	intrauterine adhesion (IUA) treatment	270:306	intrauterine adhesion (IUA) treatment	270:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	8	4	theme	area	1128:1131	arg1	rates					1133:1137	fibrosis area rates	1119:1137	fibrosis area rates in the gel therapy group and the combination therapy group	1119:1196	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	6	5	theme	inhibitory	836:845	arg1	LIF					855:857	LIF	855:857	LIF	855:857	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	6	5	theme	inhibitory	836:845	arg1	factor					847:852	leukocyte inhibitory factor	826:852	leukocyte inhibitory factor (LIF)	826:858	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	0	6	link	Autocross-linked	0:15	arg1	gel					33:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	8	7	theme	therapy	1184:1190	arg1	group					1192:1196	the combination therapy group	1168:1196	the combination therapy group	1168:1196	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	10	8	theme	combination	1454:1464	arg1	therapy					1466:1472	combination therapy	1454:1472	the combination therapy group	1450:1478	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	3	9	theme	following	520:528	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	9	theme	following	520:528	arg1	group					567:571	gel therapy group	555:571	gel therapy group	555:571	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	9	theme	following	520:528	arg1	group					548:552	IUA model group	538:552	IUA model group	538:552	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	9	theme	following	520:528	arg1	group					598:602	combination therapy group	578:602	combination therapy group (HA gel combined with ASCs)	578:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	1	10	theme	stem	203:206	arg1	cells					208:212	adipose-derived mesenchymal stem cells	175:212	adipose-derived mesenchymal stem cells (ASCs)	175:219	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	1	10	theme	stem	203:206	arg1	ASCs					215:218	ASCs	215:218	ASCs	215:218	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	7	11	from	RESULTS	952:958	arg1	group					1001:1005	the gel therapy group	985:1005	the gel therapy group	985:1005	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	11	from	RESULTS	952:958	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	11	from	RESULTS	952:958	arg1	group					1035:1039	the combination therapy group	1011:1039	the combination therapy group	1011:1039	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	8	12	theme	gel	1146:1148	arg1	group					1158:1162	the gel therapy group	1142:1162	the gel therapy group	1142:1162	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	3	13	theme	model	542:546	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	13	theme	model	542:546	arg1	group					548:552	IUA model group	538:552	IUA model group	538:552	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	5	14	theme	hematoxylin-eosin	768:784	arg1	staining					786:793	hematoxylin-eosin staining	768:793	hematoxylin-eosin staining	768:793	Morphological changes were determined by hematoxylin-eosin staining and Masson staining.					
34794733	6	15	theme	polymerase	900:909	arg1	reaction					917:924	quantitative real-time polymerase chain reaction	877:924	quantitative real-time polymerase chain reaction	877:924	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	7	16	theme	combination	1015:1025	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	16	theme	combination	1015:1025	arg1	group					1035:1039	the combination therapy group	1011:1039	the combination therapy group	1011:1039	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	1	17	theme	autocross-linked	224:239	arg1	gel					262:264	autocross-linked hyaluronic acid (HA) gel	224:264	autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment	224:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	12	18	theme	IUA	1812:1814	arg1	formation					1825:1833	the IUA adhesion formation	1808:1833	the IUA adhesion formation	1808:1833	CONCLUSIONS HA gel was effective in preventing the IUA adhesion formation at the early stage of the observation period, while ASC enhanced this effect throughout the observation period.					
34794733	6	19	theme	quantitative	877:888	arg1	reaction					917:924	quantitative real-time polymerase chain reaction	877:924	quantitative real-time polymerase chain reaction	877:924	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	11	20	from	LIF	1612:1614	arg1	group					1643:1647	the combination therapy group	1619:1647	the combination therapy group	1619:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	21	theme	model	1677:1681	arg1	group					1683:1687	the model group	1673:1687	the model group	1673:1687	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	3	22	theme	therapy	559:565	arg1	group					567:571	gel therapy group	555:571	gel therapy group	555:571	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	22	theme	therapy	559:565	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	7	23	theme	endometrial	964:974	arg1	group					1001:1005	the gel therapy group	985:1005	the gel therapy group	985:1005	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	23	theme	endometrial	964:974	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	23	theme	endometrial	964:974	arg1	group					1035:1039	the combination therapy group	1011:1039	the combination therapy group	1011:1039	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	24	theme	therapy	993:999	arg1	group					1001:1005	the gel therapy group	985:1005	the gel therapy group	985:1005	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	24	theme	therapy	993:999	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	11	25	theme	LIF	1612:1614	arg1	level					1603:1607	The mRNA expression level	1583:1607	The mRNA expression level of LIF in the combination therapy group	1583:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	25	theme	LIF	1612:1614	arg1	higher					1653:1658	higher	1653:1658	higher	1653:1658	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	0	26	theme	cell	74:77	arg1	composites					79:88	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites	0:88	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.	0:130	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	3	27	theme	combination	578:588	arg1	therapy					590:596	combination therapy	578:596	combination therapy group (HA gel combined with ASCs)	578:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	11	28	from	level	1603:1607	arg1	group					1643:1647	the combination therapy group	1619:1647	the combination therapy group	1619:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	1	29	theme	adipose-derived	175:189	arg1	cells					208:212	adipose-derived mesenchymal stem cells	175:212	adipose-derived mesenchymal stem cells (ASCs)	175:219	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	1	29	theme	adipose-derived	175:189	arg1	ASCs					215:218	ASCs	215:218	ASCs	215:218	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	12	30	theme	observation	1927:1937	arg1	period					1939:1944	the observation period	1923:1944	the observation period	1923:1944	CONCLUSIONS HA gel was effective in preventing the IUA adhesion formation at the early stage of the observation period, while ASC enhanced this effect throughout the observation period.					
34794733	11	31	theme	therapy	1635:1641	arg1	group					1643:1647	the combination therapy group	1619:1647	the combination therapy group	1619:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	0	32	theme	treatment	98:106	arg1	adhesions					121:129	the treatment intrauterine adhesions	94:129	the treatment intrauterine adhesions	94:129	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	8	33	theme	model	1241:1245	arg1	group					1247:1251	the model group	1237:1251	the model group	1237:1251	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	7	34	dep	lines	976:980	arg1	group					1001:1005	the gel therapy group	985:1005	the gel therapy group	985:1005	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	34	dep	lines	976:980	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	34	dep	lines	976:980	arg1	group					1035:1039	the combination therapy group	1011:1039	the combination therapy group	1011:1039	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	9	35	theme	therapy	1347:1353	arg1	group					1355:1359	the combination therapy group	1331:1359	the combination therapy group	1331:1359	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	11	36	theme	combination	1623:1633	arg1	group					1643:1647	the combination therapy group	1619:1647	the combination therapy group	1619:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	10	37	theme	therapy	1527:1533	arg1	group					1535:1539	gel therapy group	1523:1539	gel therapy group	1523:1539	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	0	38	theme	Autocross-linked	0:15	arg1	gel					33:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	1	39	theme	adhesion	283:290	arg1	treatment					298:306	intrauterine adhesion (IUA) treatment	270:306	intrauterine adhesion (IUA) treatment	270:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	0	40	theme	acid	28:31	arg1	gel					33:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	1	41	theme	IUA	293:295	arg1	treatment					298:306	intrauterine adhesion (IUA) treatment	270:306	intrauterine adhesion (IUA) treatment	270:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	2	42	theme	different	397:405	arg1	treatments					407:416	then different treatments	392:416	then different treatments	392:416	METHODS A rat IUA model was established by mechanical curettage and infection, and then different treatments were administered to the rats on day 7 after modeling.					
34794733	10	43	theme	observation	1552:1562	arg1	P < 0.05					1572:1579	P < 0.05	1572:1579	P < 0.05	1572:1579	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	10	43	theme	observation	1552:1562	arg1	period					1564:1569	the observation period	1548:1569	the observation period (P < 0.05)	1548:1580	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	5	44	theme	Masson	799:804	arg1	staining					806:813	Masson staining	799:813	Masson staining	799:813	Morphological changes were determined by hematoxylin-eosin staining and Masson staining.					
34794733	3	45	theme	HA	605:606	arg1	gel					608:610	HA gel	605:610	HA gel combined with ASCs	605:629	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	45	theme	HA	605:606	arg1	group					598:602	combination therapy group	578:602	combination therapy group (HA gel combined with ASCs)	578:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	46	theme	Ninety-six	473:482	arg1	rats					484:487	Ninety-six rats	473:487	Ninety-six rats	473:487	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	0	47	theme	mesenchymal	57:67	arg1	cell					74:77	adipose-derived mesenchymal stem cell	41:77	adipose-derived mesenchymal stem cell	41:77	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	9	48	from	rates	1322:1326	arg1	group					1355:1359	the combination therapy group	1331:1359	the combination therapy group	1331:1359	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	4	49	theme	subsequent	703:712	arg1	experiments					714:724	the subsequent experiments	699:724	the subsequent experiments	699:724	Eight rats per group were sacrificed on days 7, 10, 14 and 21 for the subsequent experiments.					
34794733	11	50	theme	gel	1697:1699	arg1	group					1709:1713	the gel therapy group	1693:1713	the gel therapy group	1693:1713	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	1	51	theme	hyaluronic	241:250	arg1	HA					258:259	HA	258:259	HA	258:259	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	1	51	theme	hyaluronic	241:250	arg1	acid					252:255	hyaluronic acid	241:255	autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment	224:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	11	52	theme	expression	1592:1601	arg1	level					1603:1607	The mRNA expression level	1583:1607	The mRNA expression level of LIF in the combination therapy group	1583:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	52	theme	expression	1592:1601	arg1	higher					1653:1658	higher	1653:1658	higher	1653:1658	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	9	53	theme	area	1317:1320	arg1	rates					1322:1326	the fibrosis area rates	1304:1326	the fibrosis area rates in the combination therapy group	1304:1359	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	12	54	theme	observation	1861:1871	arg1	period					1873:1878	the observation period	1857:1878	the observation period	1857:1878	CONCLUSIONS HA gel was effective in preventing the IUA adhesion formation at the early stage of the observation period, while ASC enhanced this effect throughout the observation period.					
34794733	10	55	theme	Smad3	1441:1445	arg1	lower					1484:1488	lower	1484:1488	lower	1484:1488	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	10	55	theme	Smad3	1441:1445	arg1	expression					1427:1436	The mRNA expression	1418:1436	The mRNA expression of Smad3 in the combination therapy group	1418:1478	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	13	56	theme	endometrial	1988:1998	arg1	receptivity					2000:2010	endometrial receptivity	1988:2010	endometrial receptivity	1988:2010	Gel and ASC composites helped to improve endometrial receptivity.					
34794733	8	57	theme	Masson	1091:1096	arg1	staining					1098:1105	Masson staining	1091:1105	Masson staining	1091:1105	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	9	58	theme	combination	1335:1345	arg1	therapy					1347:1353	combination therapy	1335:1353	the combination therapy group	1331:1359	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	12	59	theme	early	1842:1846	arg1	stage					1848:1852	the early stage	1838:1852	the early stage of the observation period	1838:1878	CONCLUSIONS HA gel was effective in preventing the IUA adhesion formation at the early stage of the observation period, while ASC enhanced this effect throughout the observation period.					
34794733	13	60	dep	Gel	1947:1949	arg1	composites					1959:1968	composites	1959:1968	composites	1959:1968	Gel and ASC composites helped to improve endometrial receptivity.					
34794733	10	61	theme	therapy	1466:1472	arg1	group					1474:1478	the combination therapy group	1450:1478	the combination therapy group	1450:1478	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	1	62	theme	mesenchymal	191:201	arg1	cells					208:212	adipose-derived mesenchymal stem cells	175:212	adipose-derived mesenchymal stem cells (ASCs)	175:219	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	1	62	theme	mesenchymal	191:201	arg1	ASCs					215:218	ASCs	215:218	ASCs	215:218	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	8	63	theme	fibrosis	1119:1126	arg1	area					1128:1131	fibrosis area	1119:1131	fibrosis area rates in the gel therapy group and the combination therapy group	1119:1196	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	6	64	theme	leukocyte	826:834	arg1	LIF					855:857	LIF	855:857	LIF	855:857	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	6	64	theme	leukocyte	826:834	arg1	factor					847:852	leukocyte inhibitory factor	826:852	leukocyte inhibitory factor (LIF)	826:858	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	8	65	theme	combination	1172:1182	arg1	group					1192:1196	the combination therapy group	1168:1196	the combination therapy group	1168:1196	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	12	66	theme	adhesion	1816:1823	arg1	formation					1825:1833	the IUA adhesion formation	1808:1833	the IUA adhesion formation	1808:1833	CONCLUSIONS HA gel was effective in preventing the IUA adhesion formation at the early stage of the observation period, while ASC enhanced this effect throughout the observation period.					
34794733	0	67	link	adipose-derived	41:55	arg1	cell					74:77	adipose-derived mesenchymal stem cell	41:77	adipose-derived mesenchymal stem cell	41:77	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	7	68	theme	therapy	1027:1033	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	68	theme	therapy	1027:1033	arg1	group					1035:1039	the combination therapy group	1011:1039	the combination therapy group	1011:1039	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	3	69	theme	IUA	538:540	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	69	theme	IUA	538:540	arg1	group					548:552	IUA model group	538:552	IUA model group	538:552	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	6	70	theme	chain	911:915	arg1	reaction					917:924	quantitative real-time polymerase chain reaction	877:924	quantitative real-time polymerase chain reaction	877:924	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	11	71	from	group	1643:1647	arg1	level					1603:1607	The mRNA expression level	1583:1607	The mRNA expression level of LIF in the combination therapy group	1583:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	71	from	group	1643:1647	arg1	higher					1653:1658	higher	1653:1658	higher	1653:1658	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	8	72	theme	therapy	1150:1156	arg1	group					1158:1162	the gel therapy group	1142:1162	the gel therapy group	1142:1162	Masson staining showed that fibrosis area rates in the gel therapy group and the combination therapy group were significantly lower than those in the model group on day 7(P < 0.05).					
34794733	6	73	theme	real-time	890:898	arg1	reaction					917:924	quantitative real-time polymerase chain reaction	877:924	quantitative real-time polymerase chain reaction	877:924	Smad3 and leukocyte inhibitory factor (LIF) were analyzed by quantitative real-time polymerase chain reaction and immunohistochemistry.					
34794733	10	74	theme	model	1507:1511	arg1	group					1513:1517	the model group	1503:1517	the model group	1503:1517	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	3	75	theme	gel	555:557	arg1	group					567:571	gel therapy group	555:571	gel therapy group	555:571	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	75	theme	gel	555:557	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	7	76	theme	gel	989:991	arg1	group					1001:1005	the gel therapy group	985:1005	the gel therapy group	985:1005	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	7	76	theme	gel	989:991	arg1	lines					976:980	The endometrial lines	960:980	The endometrial lines	960:980	RESULTS The endometrial lines in the gel therapy group and the combination therapy group were more complete than those in the model group.					
34794733	3	77	theme	therapy	590:596	arg1	gel					608:610	HA gel	605:610	HA gel combined with ASCs	605:629	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	77	theme	therapy	590:596	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	77	theme	therapy	590:596	arg1	group					598:602	combination therapy group	578:602	combination therapy group (HA gel combined with ASCs)	578:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	78	dep	groups	530:535	arg1	group					567:571	gel therapy group	555:571	gel therapy group	555:571	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	78	dep	groups	530:535	arg1	gel					608:610	HA gel	605:610	HA gel combined with ASCs	605:629	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	78	dep	groups	530:535	arg1	groups					530:535	the following groups	516:535	the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs)	516:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	78	dep	groups	530:535	arg1	group					548:552	IUA model group	538:552	IUA model group	538:552	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	3	78	dep	groups	530:535	arg1	group					598:602	combination therapy group	578:602	combination therapy group (HA gel combined with ASCs)	578:630	Ninety-six rats were randomly divided into the following groups: IUA model group, gel therapy group, and combination therapy group (HA gel combined with ASCs).					
34794733	2	79	dep	METHODS	309:315	arg1	administered					423:434	administered	423:434	were administered to the rats on day 7 after modeling	418:470	METHODS A rat IUA model was established by mechanical curettage and infection, and then different treatments were administered to the rats on day 7 after modeling.					
34794733	2	79	dep	METHODS	309:315	arg1	established					337:347	established	337:347	was established by mechanical curettage and infection	333:385	METHODS A rat IUA model was established by mechanical curettage and infection, and then different treatments were administered to the rats on day 7 after modeling.					
34794733	2	80	theme	IUA	323:325	arg1	model					327:331	A rat IUA model	317:331	A rat IUA model	317:331	METHODS A rat IUA model was established by mechanical curettage and infection, and then different treatments were administered to the rats on day 7 after modeling.					
34794733	1	81	link	autocross-linked	224:239	arg1	gel					262:264	autocross-linked hyaluronic acid (HA) gel	224:264	autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment	224:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	0	82	theme	intrauterine	108:119	arg1	adhesions					121:129	the treatment intrauterine adhesions	94:129	the treatment intrauterine adhesions	94:129	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	10	83	from	expression	1427:1436	arg1	group					1474:1478	the combination therapy group	1450:1478	the combination therapy group	1450:1478	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	2	84	theme	rat	319:321	arg1	model					327:331	A rat IUA model	317:331	A rat IUA model	317:331	METHODS A rat IUA model was established by mechanical curettage and infection, and then different treatments were administered to the rats on day 7 after modeling.					
34794733	1	85	theme	acid	252:255	arg1	gel					262:264	autocross-linked hyaluronic acid (HA) gel	224:264	autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment	224:306	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	5	86	theme	Morphological	727:739	arg1	changes					741:747	Morphological changes	727:747	Morphological changes	727:747	Morphological changes were determined by hematoxylin-eosin staining and Masson staining.					
34794733	10	87	theme	gel	1523:1525	arg1	group					1535:1539	gel therapy group	1523:1539	gel therapy group	1523:1539	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	0	88	theme	gel	33:35	arg1	composites					79:88	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites	0:88	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.	0:130	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	1	89	link	adipose-derived	175:189	arg1	cells					208:212	adipose-derived mesenchymal stem cells	175:212	adipose-derived mesenchymal stem cells (ASCs)	175:219	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	1	89	link	adipose-derived	175:189	arg1	ASCs					215:218	ASCs	215:218	ASCs	215:218	OBJECTIVE To evaluate the effect of adding adipose-derived mesenchymal stem cells (ASCs) to autocross-linked hyaluronic acid (HA) gel for intrauterine adhesion (IUA) treatment.					
34794733	0	90	theme	adipose-derived	41:55	arg1	cell					74:77	adipose-derived mesenchymal stem cell	41:77	adipose-derived mesenchymal stem cell	41:77	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	0	91	theme	stem	69:72	arg1	cell					74:77	adipose-derived mesenchymal stem cell	41:77	adipose-derived mesenchymal stem cell	41:77	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
34794733	9	92	theme	observation	1284:1294	arg1	period					1296:1301	the observation period	1280:1301	the observation period	1280:1301	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	2	93	theme	mechanical	352:361	arg1	curettage					363:371	mechanical curettage	352:371	mechanical curettage	352:371	METHODS A rat IUA model was established by mechanical curettage and infection, and then different treatments were administered to the rats on day 7 after modeling.					
34794733	10	94	theme	mRNA	1422:1425	arg1	lower					1484:1488	lower	1484:1488	lower	1484:1488	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	10	94	theme	mRNA	1422:1425	arg1	expression					1427:1436	The mRNA expression	1418:1436	The mRNA expression of Smad3 in the combination therapy group	1418:1478	The mRNA expression of Smad3 in the combination therapy group was lower than that in the model group and gel therapy group during the observation period (P < 0.05).					
34794733	11	95	theme	mRNA	1587:1590	arg1	level					1603:1607	The mRNA expression level	1583:1607	The mRNA expression level of LIF in the combination therapy group	1583:1647	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	95	theme	mRNA	1587:1590	arg1	higher					1653:1658	higher	1653:1658	higher	1653:1658	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	11	96	theme	therapy	1701:1707	arg1	group					1709:1713	the gel therapy group	1693:1713	the gel therapy group	1693:1713	The mRNA expression level of LIF in the combination therapy group was higher than that in the model group and the gel therapy group throughout the observation period (P < 0.05).					
34794733	9	97	theme	fibrosis	1308:1315	arg1	area					1317:1320	fibrosis area	1308:1320	the fibrosis area rates in the combination therapy group	1304:1359	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	12	98	theme	period	1873:1878	arg1	stage					1848:1852	the early stage	1838:1852	the early stage of the observation period	1838:1878	CONCLUSIONS HA gel was effective in preventing the IUA adhesion formation at the early stage of the observation period, while ASC enhanced this effect throughout the observation period.					
34794733	9	99	theme	model	1394:1398	arg1	P < 0.05					1407:1414	P < 0.05	1407:1414	P < 0.05	1407:1414	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	9	99	theme	model	1394:1398	arg1	group					1400:1404	the model group	1390:1404	the model group (P < 0.05)	1390:1415	During the observation period, the fibrosis area rates in the combination therapy group remained lower than those in the model group (P < 0.05).					
34794733	0	100	theme	hyaluronic	17:26	arg1	gel					33:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel	0:35	Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.					
33314156	10	0	theme	protein	1929:1935	arg1	supply					1937:1942	a protein supply	1927:1942	a protein supply of adequate quality in mostly plant-based diets	1927:1990	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	3	1	theme	pasta	559:563	arg1	quality					538:544	the nutritional quality	522:544	the nutritional quality of fortified pasta	522:563	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	1	2	theme	growing	274:280	arg1	interest					291:298	growing research interest	274:298	growing research interest in recent years	274:314	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	7	3	theme	enhanced	1476:1483	arg1	quality					1493:1499	a substantially enhanced protein quality	1460:1499	a substantially enhanced protein quality	1460:1499	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	10	4	theme	quality	1956:1962	arg1	supply					1937:1942	a protein supply	1927:1942	a protein supply of adequate quality in mostly plant-based diets	1927:1990	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	5	theme	value	1780:1784	arg1	upgrade					1749:1755	This significant upgrade	1732:1755	This significant upgrade of pasta's nutritional value	1732:1784	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	1	6	theme	staple	196:201	arg1	foods					203:207	wheat-based staple foods	184:207	wheat-based staple foods	184:207	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	1	6	theme	staple	196:201	arg1	pasta					218:222	pasta	218:222	pasta	218:222	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	4	7	theme	faba	857:860	arg1	bean					862:865	faba bean	857:865	faba bean	857:865	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	6	8	theme	wheat	1179:1183	arg1	pasta					1185:1189	regular wheat pasta	1171:1189	regular wheat pasta	1171:1189	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	1	9	from	interest	291:298	arg1	years					310:314	recent years	303:314	recent years	303:314	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	10	10	theme	hybrid	1815:1820	arg1	HPHP					1797:1800	HPHP	1797:1800	HPHP	1797:1800	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	10	theme	hybrid	1815:1820	arg1	choice					1854:1859	a healthy food choice	1839:1859	a healthy food choice	1839:1859	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	10	theme	hybrid	1815:1820	arg1	formulations					1822:1833	similar hybrid formulations	1807:1833	similar hybrid formulations	1807:1833	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	11	dep	CONCLUSION	1721:1730	arg1	identifies					1786:1795	identifies	1786:1795	identifies	1786:1795	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	0	12	theme	enhanced	122:129	arg1	profile					143:149	enhanced nutritional profile	122:149	enhanced nutritional profile	122:149	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	6	13	theme	starch	1256:1261	arg1	replacement					1235:1245	the isocaloric replacement	1220:1245	the isocaloric replacement of wheat starch by non-wheat protein	1220:1282	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	1	14	dep	pseudocereal	230:241	arg1	flours					254:259	flours	254:259	flours	254:259	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	7	15	theme	protein	1371:1377	arg1	ratio					1390:1394	increased protein efficiency ratio	1361:1394	increased protein efficiency ratio (in vivo)	1361:1404	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	6	16	theme	isocaloric	1224:1233	arg1	replacement					1235:1245	the isocaloric replacement	1220:1245	the isocaloric replacement of wheat starch by non-wheat protein	1220:1282	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	4	17	theme	hybrid	745:750	arg1	formulation					765:775	a high-protein hybrid pasta (HPHP) formulation	730:775	a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina	730:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	5	18	theme	regular	1058:1064	arg1	pasta					1072:1076	regular wheat pasta	1058:1076	regular wheat pasta	1058:1076	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	11	19	theme	Chemical	2011:2018	arg1	Industry					2020:2027	Chemical Industry	2011:2027	Chemical Industry	2011:2027	© 2020 Society of Chemical Industry.					
33314156	10	20	theme	food	1849:1852	arg1	HPHP					1797:1800	HPHP	1797:1800	HPHP	1797:1800	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	20	theme	food	1849:1852	arg1	choice					1854:1859	a healthy food choice	1839:1859	a healthy food choice	1839:1859	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	20	theme	food	1849:1852	arg1	formulations					1822:1833	similar hybrid formulations	1807:1833	similar hybrid formulations	1807:1833	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	8	21	theme	cooking	1506:1512	arg1	process					1514:1520	The cooking process	1502:1520	The cooking process	1502:1520	The cooking process was shown to significantly reduce levels of vicine/convicine and trypsin inhibitor activity originating from HPIs.					
33314156	4	22	theme	HPHP	759:762	arg1	formulation					765:775	a high-protein hybrid pasta (HPHP) formulation	730:775	a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina	730:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	0	23	theme	high-protein	95:106	arg1	pasta					115:119	fresh high-protein hybrid pasta	89:119	fresh high-protein hybrid pasta	89:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	5	24	theme	protein	967:973	arg1	quality					975:981	protein quality	967:981	protein quality	967:981	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	0	25	from	legumes	58:64	arg1	ingredients					23:33	high-protein ingredients	10:33	high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta	10:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	0	26	theme	pasta	115:119	arg1	development					74:84	the development	70:84	the development of fresh high-protein hybrid pasta	70:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	4	27	from	buckwheat	846:854	arg1	combination					788:798	a combination	786:798	a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	786:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	27	from	buckwheat	846:854	arg1	HPIs					835:838	HPIs	835:838	HPIs	835:838	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	27	from	buckwheat	846:854	arg1	ingredients					822:832	three high-protein ingredients	803:832	three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	803:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	0	28	from	pseudocereals	40:52	arg1	ingredients					23:33	high-protein ingredients	10:33	high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta	10:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	1	29	theme	research	282:289	arg1	interest					291:298	growing research interest	274:298	growing research interest in recent years	274:314	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	7	30	theme	improved	1334:1341	arg1	utilisation					1345:1355	improved N utilisation	1334:1355	improved N utilisation	1334:1355	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	2	31	theme	substantial	436:446	arg1	improvement					448:458	a substantial improvement	434:458	a substantial improvement of the nutritional value of pasta	434:492	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	1	32	dep	BACKGROUND	152:161	arg1	received					265:272	received	265:272	has received growing research interest in recent years	261:314	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	10	33	theme	regular	1889:1895	arg1	pasta					1903:1907	regular wheat pasta	1889:1907	regular wheat pasta	1889:1907	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	7	34	dep	ratio	1390:1394	arg1	vivo					1400:1403	in vivo	1397:1403	in vivo	1397:1403	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	7	35	theme	amino	1314:1318	arg1	profile					1325:1331	a more balanced amino acid profile	1298:1331	a more balanced amino acid profile	1298:1331	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	2	36	theme	nutritional	467:477	arg1	value					479:483	the nutritional value	463:483	the nutritional value of pasta	463:492	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	8	37	theme	inhibitor	1595:1603	arg1	activity					1605:1612	trypsin inhibitor activity	1587:1612	trypsin inhibitor activity	1587:1612	The cooking process was shown to significantly reduce levels of vicine/convicine and trypsin inhibitor activity originating from HPIs.					
33314156	2	38	theme	many	345:348	arg1	challenges					350:359	many challenges	345:359	many challenges regarding technological and sensory quality of the products	345:419	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	9	39	theme	small	1641:1645	arg1	levels					1657:1662	The small remaining levels	1637:1662	The small remaining levels	1637:1662	The small remaining levels seem not to adversely affect HPHP's nutritional quality.					
33314156	6	40	theme	macronutrient	1137:1149	arg1	profile					1151:1157	a more favourable macronutrient profile	1119:1157	a more favourable macronutrient profile	1119:1157	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	4	41	theme	high-protein	809:820	arg1	HPIs					835:838	HPIs	835:838	HPIs	835:838	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	41	theme	high-protein	809:820	arg1	ingredients					822:832	three high-protein ingredients	803:832	three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	803:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	10	42	theme	nutritional	1768:1778	arg1	value					1780:1784	pasta's nutritional value	1760:1784	pasta's nutritional value	1760:1784	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	5	43	theme	antinutritional	1002:1016	arg1	compounds					1018:1026	antinutritional compounds	1002:1026	antinutritional compounds	1002:1026	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	7	44	theme	protein	1485:1491	arg1	quality					1493:1499	a substantially enhanced protein quality	1460:1499	a substantially enhanced protein quality	1460:1499	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	6	45	theme	HPHP	1091:1094	arg1	formulation					1096:1106	The HPHP formulation	1087:1106	The HPHP formulation	1087:1106	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	3	46	theme	fortified	549:557	arg1	pasta					559:563	fortified pasta	549:563	fortified pasta	549:563	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	4	47	used	used	880:883	arg2	combination					788:798	a combination	786:798	a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	786:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	3	48	theme	quality	538:544	arg1	investigations					504:517	investigations	504:517	investigations of the nutritional quality of fortified pasta	504:563	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	1	49	theme	wheat-based	184:194	arg1	foods					203:207	wheat-based staple foods	184:207	wheat-based staple foods	184:207	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	1	49	theme	wheat-based	184:194	arg1	pasta					218:222	pasta	218:222	pasta	218:222	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	9	50	theme	nutritional	1700:1710	arg1	quality					1712:1718	HPHP's nutritional quality	1693:1718	HPHP's nutritional quality	1693:1718	The small remaining levels seem not to adversely affect HPHP's nutritional quality.					
33314156	10	51	theme	adequate	1947:1954	arg1	quality					1956:1962	adequate quality	1947:1962	adequate quality	1947:1962	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	6	52	theme	non-wheat	1266:1274	arg1	protein					1276:1282	non-wheat protein	1266:1282	non-wheat protein	1266:1282	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	1	53	theme	foods	203:207	arg1	fortification					167:179	The fortification	163:179	The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours	163:259	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	6	54	theme	regular	1171:1177	arg1	pasta					1185:1189	regular wheat pasta	1171:1189	regular wheat pasta	1171:1189	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	0	55	theme	nutritional	131:141	arg1	profile					143:149	enhanced nutritional profile	122:149	enhanced nutritional profile	122:149	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	10	56	theme	similar	1807:1813	arg1	HPHP					1797:1800	HPHP	1797:1800	HPHP	1797:1800	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	56	theme	similar	1807:1813	arg1	choice					1854:1859	a healthy food choice	1839:1859	a healthy food choice	1839:1859	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	56	theme	similar	1807:1813	arg1	formulations					1822:1833	similar hybrid formulations	1807:1833	similar hybrid formulations	1807:1833	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	57	from	supply	1937:1942	arg1	diets					1986:1990	mostly plant-based diets	1967:1990	mostly plant-based diets	1967:1990	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	4	58	theme	wheat	906:910	arg1	semolina					912:919	wheat semolina	906:919	wheat semolina	906:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	10	59	theme	plant-based	1974:1984	arg1	diets					1986:1990	mostly plant-based diets	1967:1990	mostly plant-based diets	1967:1990	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	60	theme	healthy	1841:1847	arg1	HPHP					1797:1800	HPHP	1797:1800	HPHP	1797:1800	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	60	theme	healthy	1841:1847	arg1	choice					1854:1859	a healthy food choice	1839:1859	a healthy food choice	1839:1859	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	10	60	theme	healthy	1841:1847	arg1	formulations					1822:1833	similar hybrid formulations	1807:1833	similar hybrid formulations	1807:1833	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	6	61	theme	wheat	1250:1254	arg1	starch					1256:1261	wheat starch	1250:1261	wheat starch	1250:1261	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	3	62	theme	carbohydrate/starch	584:602	arg1	fraction					604:611	the carbohydrate/starch fraction	580:611	the carbohydrate/starch fraction	580:611	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	10	63	theme	valuable	1865:1872	arg1	alternative					1874:1884	valuable alternative	1865:1884	valuable alternative to regular wheat pasta	1865:1907	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	7	64	theme	efficiency	1379:1388	arg1	ratio					1390:1394	increased protein efficiency ratio	1361:1394	increased protein efficiency ratio (in vivo)	1361:1404	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	4	65	theme	high-protein	732:743	arg1	formulation					765:775	a high-protein hybrid pasta (HPHP) formulation	730:775	a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina	730:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	5	66	theme	wheat	1066:1070	arg1	pasta					1072:1076	regular wheat pasta	1058:1076	regular wheat pasta	1058:1076	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	7	67	theme	increased	1361:1369	arg1	ratio					1390:1394	increased protein efficiency ratio	1361:1394	increased protein efficiency ratio (in vivo)	1361:1404	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	0	68	theme	fresh	89:93	arg1	pasta					115:119	fresh high-protein hybrid pasta	89:119	fresh high-protein hybrid pasta	89:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	4	69	from	lupin	871:875	arg1	combination					788:798	a combination	786:798	a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	786:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	69	from	lupin	871:875	arg1	HPIs					835:838	HPIs	835:838	HPIs	835:838	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	69	from	lupin	871:875	arg1	ingredients					822:832	three high-protein ingredients	803:832	three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	803:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	1	70	with	fortification	167:179	arg1	legume					247:252	legume	247:252	legume	247:252	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	1	70	with	fortification	167:179	arg1	pseudocereal					230:241	pseudocereal	230:241	pseudocereal	230:241	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	4	71	from	combination	788:798	arg1	lupin					871:875	lupin	871:875	lupin	871:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	71	from	combination	788:798	arg1	buckwheat					846:854	buckwheat	846:854	buckwheat	846:854	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	71	from	combination	788:798	arg1	bean					862:865	faba bean	857:865	faba bean	857:865	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	2	72	theme	pasta	488:492	arg1	value					479:483	the nutritional value	463:483	the nutritional value of pasta	463:492	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	4	73	theme	nutritional	707:717	arg1	profile					719:725	the nutritional profile	703:725	the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina	703:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	0	74	theme	hybrid	108:113	arg1	pasta					115:119	fresh high-protein hybrid pasta	89:119	fresh high-protein hybrid pasta	89:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	4	75	from	bean	862:865	arg1	combination					788:798	a combination	786:798	a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	786:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	75	from	bean	862:865	arg1	HPIs					835:838	HPIs	835:838	HPIs	835:838	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	75	from	bean	862:865	arg1	ingredients					822:832	three high-protein ingredients	803:832	three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	803:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	4	76	theme	formulation	765:775	arg1	profile					719:725	the nutritional profile	703:725	the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina	703:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	3	77	from	information	618:628	arg1	changes					633:639	changes	633:639	changes in protein quality	633:658	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	7	78	theme	N	1343:1343	arg1	utilisation					1345:1355	improved N utilisation	1334:1355	improved N utilisation	1334:1355	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	3	79	from	changes	633:639	arg1	quality					652:658	protein quality	644:658	protein quality	644:658	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	0	80	theme	high-protein	10:21	arg1	ingredients					23:33	high-protein ingredients	10:33	high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta	10:119	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	8	81	theme	vicine/convicine	1566:1581	arg1	levels					1556:1561	levels	1556:1561	levels of vicine/convicine and trypsin inhibitor activity originating from HPIs	1556:1634	The cooking process was shown to significantly reduce levels of vicine/convicine and trypsin inhibitor activity originating from HPIs.					
33314156	4	82	theme	pasta	752:756	arg1	formulation					765:775	a high-protein hybrid pasta (HPHP) formulation	730:775	a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina	730:919	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	2	83	theme	sensory	389:395	arg1	quality					397:403	technological and sensory quality	371:403	technological and sensory quality of the products	371:419	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	5	84	theme	macronutrient	940:952	arg1	composition					954:964	The formulation's macronutrient composition	922:964	The formulation's macronutrient composition	922:964	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	7	85	theme	acid	1320:1323	arg1	profile					1325:1331	a more balanced amino acid profile	1298:1331	a more balanced amino acid profile	1298:1331	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	8	86	theme	trypsin	1587:1593	arg1	activity					1605:1612	trypsin inhibitor activity	1587:1612	trypsin inhibitor activity	1587:1612	The cooking process was shown to significantly reduce levels of vicine/convicine and trypsin inhibitor activity originating from HPIs.					
33314156	10	87	theme	wheat	1897:1901	arg1	pasta					1903:1907	regular wheat pasta	1889:1907	regular wheat pasta	1889:1907	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	6	88	dep	RESULTS	1079:1085	arg1	represents					1108:1117	represents	1108:1117	represents	1108:1117	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	1	89	theme	recent	303:308	arg1	years					310:314	recent years	303:314	recent years	303:314	BACKGROUND The fortification of wheat-based staple foods, such as pasta, with pseudocereal and legume flours has received growing research interest in recent years.					
33314156	5	90	theme	compounds	1018:1026	arg1	composition					954:964	The formulation's macronutrient composition	922:964	The formulation's macronutrient composition	922:964	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	5	90	theme	compounds	1018:1026	arg1	quality					975:981	protein quality	967:981	protein quality	967:981	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	5	90	theme	compounds	1018:1026	arg1	content					991:997	the content	987:997	the content of antinutritional compounds	987:1026	The formulation's macronutrient composition, protein quality and the content of antinutritional compounds are assessed in comparison to regular wheat pasta.					
33314156	7	91	theme	balanced	1305:1312	arg1	profile					1325:1331	a more balanced amino acid profile	1298:1331	a more balanced amino acid profile	1298:1331	Furthermore, a more balanced amino acid profile, improved N utilisation and increased protein efficiency ratio (in vivo) were determined for HPHP, which conclusively suggests a substantially enhanced protein quality.					
33314156	2	92	theme	value	479:483	arg1	improvement					448:458	a substantial improvement	434:458	a substantial improvement of the nutritional value of pasta	434:492	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	8	93	theme	activity	1605:1612	arg1	levels					1556:1561	levels	1556:1561	levels of vicine/convicine and trypsin inhibitor activity originating from HPIs	1556:1634	The cooking process was shown to significantly reduce levels of vicine/convicine and trypsin inhibitor activity originating from HPIs.					
33314156	3	94	theme	protein	644:650	arg1	quality					652:658	protein quality	644:658	protein quality	644:658	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33314156	4	95	theme	ingredients	822:832	arg1	combination					788:798	a combination	786:798	a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin	786:875	This study evaluates the nutritional profile of a high-protein hybrid pasta (HPHP) formulation in which a combination of three high-protein ingredients (HPIs) from buckwheat, faba bean and lupin is used to partially replace wheat semolina.					
33314156	2	96	theme	products	412:419	arg1	quality					397:403	technological and sensory quality	371:403	technological and sensory quality of the products	371:419	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	10	97	theme	significant	1737:1747	arg1	upgrade					1749:1755	This significant upgrade	1732:1755	This significant upgrade of pasta's nutritional value	1732:1784	CONCLUSION This significant upgrade of pasta's nutritional value identifies HPHP, and similar hybrid formulations, as a healthy food choice and valuable alternative to regular wheat pasta, specifically for a protein supply of adequate quality in mostly plant-based diets.					
33314156	0	98	dep	Combining	0:8	arg1	profile					143:149	enhanced nutritional profile	122:149	enhanced nutritional profile	122:149	Combining high-protein ingredients from pseudocereals and legumes for the development of fresh high-protein hybrid pasta: enhanced nutritional profile.					
33314156	9	99	theme	remaining	1647:1655	arg1	levels					1657:1662	The small remaining levels	1637:1662	The small remaining levels	1637:1662	The small remaining levels seem not to adversely affect HPHP's nutritional quality.					
33314156	6	100	theme	favourable	1126:1135	arg1	profile					1151:1157	a more favourable macronutrient profile	1119:1157	a more favourable macronutrient profile	1119:1157	RESULTS The HPHP formulation represents a more favourable macronutrient profile compared to regular wheat pasta, particularly in relation to the isocaloric replacement of wheat starch by non-wheat protein.					
33314156	2	101	theme	technological	371:383	arg1	quality					397:403	technological and sensory quality	371:403	technological and sensory quality of the products	371:419	While it is associated with many challenges regarding technological and sensory quality of the products, it promises a substantial improvement of the nutritional value of pasta.					
33314156	3	102	theme	nutritional	526:536	arg1	quality					538:544	the nutritional quality	522:544	the nutritional quality of fortified pasta	522:563	However, investigations of the nutritional quality of fortified pasta often focus on the carbohydrate/starch fraction, and information on changes in protein quality is relatively scarce.					
33831438	0	0	theme	human	85:89	arg1	populations					91:101	diverse human populations	77:101	diverse human populations	77:101	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	7	1	theme	association	1065:1075	arg1	analyses					1077:1084	genome-wide association analyses	1053:1084	genome-wide association analyses	1053:1084	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	1	2	theme	third	143:147	arg1	oligosaccharides					115:130	Human milk oligosaccharides	104:130	Human milk oligosaccharides (HMO)	104:136	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	2	theme	third	143:147	arg1	component					163:171	the third most abundant component	139:171	the third most abundant component of human milk	139:185	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	0	3	theme	diverse	77:83	arg1	populations					91:101	diverse human populations	77:101	diverse human populations	77:101	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	7	4	theme	genome-wide	1053:1063	arg1	analyses					1077:1084	genome-wide association analyses	1053:1084	genome-wide association analyses	1053:1084	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	6	5	from	selection	977:985	arg1	FUT3					990:993	FUT3	990:993	FUT3	990:993	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	2	6	theme	Lewis	318:322	arg1	groups					343:348	Lewis and secretor blood groups	318:348	groups	343:348	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	2	6	theme	Lewis	318:322	arg1	lactation					303:311	lactation	303:311	lactation	303:311	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	1	7	theme	infant	232:237	arg1	health					239:244	infant health	232:244	infant health	232:244	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	6	8	from	polymorphisms	834:846	arg1	FUT3					851:854	FUT3	851:854	FUT3	851:854	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	4	9	theme	association	552:562	arg1	analyses					564:571	genome-wide association analyses	540:571	genome-wide association analyses of 395 women from 8 countries	540:601	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	6	10	from	FUT3	990:993	arg1	evidence					965:972	little evidence	958:972	little evidence of selection on FUT3	958:993	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	3	11	theme	HMO	460:462	arg1	composition					464:474	HMO composition	460:474	HMO composition among women	460:486	However, little is known about how variation across the genome may influence HMO composition among women in various populations.					
33831438	4	12	theme	genome-wide	540:550	arg1	analyses					564:571	genome-wide association analyses	540:571	genome-wide association analyses of 395 women from 8 countries	540:601	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	4	13	theme	different	650:658	arg1	HMO					660:662	19 different HMO	647:662	19 different HMO	647:662	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	1	14	theme	abundant	154:161	arg1	oligosaccharides					115:130	Human milk oligosaccharides	104:130	Human milk oligosaccharides (HMO)	104:136	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	14	theme	abundant	154:161	arg1	component					163:171	the third most abundant component	139:171	the third most abundant component of human milk	139:185	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	7	15	theme	use	1046:1048	arg1	report					1032:1037	the first report	1022:1037	the first report of the use of genome-wide association analyses on HMO	1022:1091	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	7	15	theme	use	1046:1048	arg1	this					1014:1017	this	1014:1017	this	1014:1017	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	3	16	theme	various	491:497	arg1	populations					499:509	various populations	491:509	various populations	491:509	However, little is known about how variation across the genome may influence HMO composition among women in various populations.					
33831438	0	17	theme	genetic	4:10	arg1	variants					12:19	Key genetic variants	0:19	Key genetic variants	0:19	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	4	18	from	analyses	564:571	arg1	countries					593:601	8 countries	591:601	8 countries	591:601	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	6	19	from	variation	882:890	arg1	lacto-N-fucopentaose					895:914	lacto-N-fucopentaose II	895:917	lacto-N-fucopentaose II	895:917	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	6	19	from	variation	882:890	arg1	difucosyllacto-N-tetrose					923:946	difucosyllacto-N-tetrose	923:946	difucosyllacto-N-tetrose	923:946	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	0	20	theme	Key	0:2	arg1	variants					12:19	Key genetic variants	0:19	Key genetic variants	0:19	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	7	21	from	report	1032:1037	arg1	HMO					1089:1091	HMO	1089:1091	HMO	1089:1091	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	5	22	dep	P < 4.5-70	739:748	arg1	to					736:737	to	736:737	to	736:737	Our data support FUT2 as the most significantly associated (P < 4.23-9 to P < 4.5-70) gene with seven HMO and provide evidence of balancing selection for FUT2.					
33831438	5	23	theme	selection	805:813	arg1	evidence					783:790	evidence	783:790	evidence of balancing selection for FUT2	783:822	Our data support FUT2 as the most significantly associated (P < 4.23-9 to P < 4.5-70) gene with seven HMO and provide evidence of balancing selection for FUT2.					
33831438	1	24	theme	human	176:180	arg1	milk					182:185	human milk	176:185	human milk	176:185	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	25	theme	Human	104:108	arg1	HMO					133:135	HMO	133:135	HMO	133:135	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	25	theme	Human	104:108	arg1	oligosaccharides					115:130	Human milk oligosaccharides	104:130	Human milk oligosaccharides (HMO)	104:136	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	25	theme	Human	104:108	arg1	component					163:171	the third most abundant component	139:171	the third most abundant component of human milk	139:185	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	4	26	from	countries	593:601	arg1	analyses					564:571	genome-wide association analyses	540:571	genome-wide association analyses of 395 women from 8 countries	540:601	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	4	26	from	countries	593:601	arg1	women					580:584	395 women	576:584	395 women from 8 countries	576:601	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	1	27	theme	milk	182:185	arg1	oligosaccharides					115:130	Human milk oligosaccharides	104:130	Human milk oligosaccharides (HMO)	104:136	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	27	theme	milk	182:185	arg1	component					163:171	the third most abundant component	139:171	the third most abundant component of human milk	139:185	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	7	28	theme	first	1026:1030	arg1	report					1032:1037	the first report	1022:1037	the first report of the use of genome-wide association analyses on HMO	1022:1091	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	7	28	theme	first	1026:1030	arg1	this					1014:1017	this	1014:1017	this	1014:1017	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	1	29	theme	milk	110:113	arg1	HMO					133:135	HMO	133:135	HMO	133:135	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	29	theme	milk	110:113	arg1	oligosaccharides					115:130	Human milk oligosaccharides	104:130	Human milk oligosaccharides (HMO)	104:136	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	1	29	theme	milk	110:113	arg1	component					163:171	the third most abundant component	139:171	the third most abundant component of human milk	139:185	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	4	30	theme	genetic	615:621	arg1	regions					623:629	genetic regions	615:629	genetic regions associated with 19 different HMO	615:662	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	5	31	dep	associated	713:722	arg1	P < 4.5-70					739:748	P < 4.5-70	739:748	P < 4.5-70	739:748	Our data support FUT2 as the most significantly associated (P < 4.23-9 to P < 4.5-70) gene with seven HMO and provide evidence of balancing selection for FUT2.					
33831438	4	32	theme	women	580:584	arg1	analyses					564:571	genome-wide association analyses	540:571	genome-wide association analyses of 395 women from 8 countries	540:601	In this study, we performed genome-wide association analyses of 395 women from 8 countries to identify genetic regions associated with 19 different HMO.					
33831438	5	33	theme	balancing	795:803	arg1	selection					805:813	balancing selection	795:813	balancing selection	795:813	Our data support FUT2 as the most significantly associated (P < 4.23-9 to P < 4.5-70) gene with seven HMO and provide evidence of balancing selection for FUT2.					
33831438	2	34	theme	HMO	370:372	arg1	profile					374:380	HMO profile	370:380	HMO profile	370:380	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	0	35	theme	milk	50:53	arg1	oligosaccharides					55:70	milk oligosaccharides	50:70	milk oligosaccharides	50:70	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	5	36	theme	associated	713:722	arg1	gene					751:754	the most significantly associated (P < 4.23-9 to P < 4.5-70) gene	690:754	the most significantly associated (P < 4.23-9 to P < 4.5-70) gene	690:754	Our data support FUT2 as the most significantly associated (P < 4.23-9 to P < 4.5-70) gene with seven HMO and provide evidence of balancing selection for FUT2.					
33831438	5	37	with	FUT2	682:685	arg1	HMO					767:769	seven HMO	761:769	seven HMO	761:769	Our data support FUT2 as the most significantly associated (P < 4.23-9 to P < 4.5-70) gene with seven HMO and provide evidence of balancing selection for FUT2.					
33831438	6	38	from	evidence	965:972	arg1	FUT3					990:993	FUT3	990:993	FUT3	990:993	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	7	39	theme	analyses	1077:1084	arg1	use					1046:1048	the use	1042:1048	the use of genome-wide association analyses	1042:1084	To our knowledge, this is the first report of the use of genome-wide association analyses on HMO.					
33831438	2	40	theme	lactation	303:311	arg1	geography					283:291	geography	283:291	geography	283:291	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	2	40	theme	lactation	303:311	arg1	groups					343:348	Lewis and secretor blood groups	318:348	groups	343:348	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	2	40	theme	lactation	303:311	arg1	lactation					303:311	lactation	303:311	lactation	303:311	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	2	40	theme	lactation	303:311	arg1	stage					294:298	stage	294:298	stage of lactation	294:311	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	6	41	theme	little	958:963	arg1	evidence					965:972	little evidence	958:972	little evidence of selection on FUT3	958:993	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
33831438	0	42	from	populations	91:101	arg1	variation					37:45	variation	37:45	variation of milk oligosaccharides from diverse human populations	37:101	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	2	43	theme	blood	337:341	arg1	groups					343:348	Lewis and secretor blood groups	318:348	groups	343:348	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	2	43	theme	blood	337:341	arg1	lactation					303:311	lactation	303:311	lactation	303:311	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	1	44	theme	important	206:214	arg1	contributors					216:227	important contributors	206:227	important contributors to infant health	206:244	Human milk oligosaccharides (HMO), the third most abundant component of human milk, are thought to be important contributors to infant health.					
33831438	0	45	theme	oligosaccharides	55:70	arg1	variation					37:45	variation	37:45	variation of milk oligosaccharides from diverse human populations	37:101	Key genetic variants associated with variation of milk oligosaccharides from diverse human populations.					
33831438	2	46	theme	secretor	328:335	arg1	groups					343:348	Lewis and secretor blood groups	318:348	groups	343:348	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	2	46	theme	secretor	328:335	arg1	lactation					303:311	lactation	303:311	lactation	303:311	Studies have provided evidence that geography, stage of lactation, and Lewis and secretor blood groups are associated with HMO profile.					
33831438	6	47	theme	selection	977:985	arg1	evidence					965:972	little evidence	958:972	little evidence of selection on FUT3	958:993	Although polymorphisms in FUT3 were also associated with variation in lacto-N-fucopentaose II and difucosyllacto-N-tetrose, we found little evidence of selection on FUT3.					
34000070	8	0	theme	significant	1154:1164	arg1	impact					1173:1178	a significant direct impact	1152:1178	a significant direct impact	1152:1178	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	10	1	from	concentrations	1612:1625	arg1	grain					1641:1645	the barley grain	1630:1645	the barley grain	1630:1645	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	6	2	theme	Thermal	826:832	arg1	stress					834:839	Thermal stress	826:839	Thermal stress	826:839	Thermal stress affected grain weight and size and changed the relative composition of bioactive compounds.					
34000070	9	3	theme	phenolic	1441:1448	arg1	concentration					1459:1471	phenolic compound concentration	1441:1471	phenolic compound concentration	1441:1471	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	6	4	theme	bioactive	912:920	arg1	compounds					922:930	bioactive compounds	912:930	bioactive compounds	912:930	Thermal stress affected grain weight and size and changed the relative composition of bioactive compounds.					
34000070	5	5	theme	post-anthesis	640:652	arg1	stress					662:667	post-anthesis thermal stress	640:667	post-anthesis thermal stress	640:667	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	2	6	theme	high	256:259	arg1	temperature					261:271	high temperature	256:271	high temperature	256:271	It faces periods of high temperature during grain filling, frequently reducing grain weight.					
34000070	9	7	theme	antioxidant	1477:1487	arg1	capacity					1489:1496	antioxidant capacity	1477:1496	antioxidant capacity of barley grains	1477:1513	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	1	8	theme	high	182:185	arg1	content					187:193	its high content	178:193	its high content of dietary fibre and phenolic compounds	178:233	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	7	9	theme	lower	1091:1095	arg1	weight					1103:1108	the lower grain weight	1087:1108	the lower grain weight under stress	1087:1121	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	8	10	contain	had	1148:1150	arg1	stress					1141:1146	heat stress	1136:1146	heat stress	1136:1146	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	8	10	contain	had	1148:1150	arg2	impact					1173:1178	a significant direct impact	1152:1178	a significant direct impact	1152:1178	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	9	11	theme	grains	1508:1513	arg1	capacity					1489:1496	antioxidant capacity	1477:1496	antioxidant capacity of barley grains	1477:1513	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	9	11	theme	grains	1508:1513	arg1	arabinoxylans					1426:1438	arabinoxylans	1426:1438	arabinoxylans	1426:1438	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	9	11	theme	grains	1508:1513	arg1	β-glucans					1415:1423	β-glucans	1415:1423	β-glucans	1415:1423	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	9	11	theme	grains	1508:1513	arg1	concentration					1459:1471	phenolic compound concentration	1441:1471	phenolic compound concentration	1441:1471	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	4	12	theme	quality	422:428	arg1	grains					430:435	quality grains	422:435	quality grains that provide nutritional and health benefits	422:480	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	5	13	theme	antioxidant	703:713	arg1	capacity					715:722	antioxidant capacity	703:722	antioxidant capacity	703:722	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	4	14	dep	quantity	557:564	arg1	the					553:555	the	553:555	the	553:555	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	1	15	theme	fibre	206:210	arg1	content					187:193	its high content	178:193	its high content of dietary fibre and phenolic compounds	178:233	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	5	16	theme	stress	662:667	arg1	effect					630:635	the effect	626:635	the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity	626:722	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	13	17	theme	John	1913:1916	arg1	Sons					1926:1929	John Wiley & Sons Ltd	1913:1933	John Wiley & Sons Ltd on behalf of Society of Chemical Industry	1913:1975	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
34000070	5	18	theme	barley	672:677	arg1	compounds					689:697	barley bioactive compounds	672:697	barley bioactive compounds	672:697	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	13	19	theme	&	1924:1924	arg1	Sons					1926:1929	John Wiley & Sons Ltd	1913:1933	John Wiley & Sons Ltd on behalf of Society of Chemical Industry	1913:1975	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
34000070	0	20	theme	compounds	76:84	arg1	accumulation					50:61	differential accumulation	37:61	differential accumulation of bioactive compounds in field-grown barley	37:106	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	7	21	theme	arabinoxylans	994:1006	arg1	content					1008:1014	arabinoxylans content	994:1014	arabinoxylans content	994:1014	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	0	22	theme	field-grown	89:99	arg1	barley					101:106	field-grown barley	89:106	field-grown barley	89:106	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	13	23	dep	Sons	1926:1929	arg1	Ltd					1931:1933	Ltd	1931:1933	Ltd	1931:1933	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
34000070	4	24	theme	nutritional	450:460	arg1	benefits					473:480	nutritional and health benefits	450:480	nutritional and health benefits	450:480	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	10	25	theme	Final	1516:1520	arg1	quality					1528:1534	Final grain quality	1516:1534	Final grain quality	1516:1534	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	10	26	theme	prone	1680:1684	arg1	areas					1674:1678	growing areas	1666:1678	growing areas prone to heat stress	1666:1699	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	5	27	theme	field	744:748	arg1	conditions					750:759	Mediterranean field conditions	730:759	Mediterranean field conditions	730:759	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	4	28	from	effect	517:522	arg1	quality					570:576	quality	570:576	quality	570:576	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	4	28	from	effect	517:522	arg1	quantity					557:564	quantity	557:564	quantity	557:564	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	7	29	theme	heat	958:961	arg1	stress					963:968	heat stress	958:968	heat stress	958:968	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	4	30	theme	health	466:471	arg1	benefits					473:480	nutritional and health benefits	450:480	nutritional and health benefits	450:480	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	10	31	theme	growing	1666:1672	arg1	areas					1674:1678	growing areas	1666:1678	growing areas prone to heat stress	1666:1699	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	0	32	theme	Post-anthesis	0:12	arg1	stress					22:27	Post-anthesis thermal stress	0:27	Post-anthesis thermal stress	0:27	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	9	33	theme	CONCLUSION	1347:1356	arg1	stress					1380:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	1	34	theme	BACKGROUND	109:118	arg1	Barley					120:125	BACKGROUND Barley	109:125	BACKGROUND Barley (Hordeum vulgare L.)	109:146	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	1	34	theme	BACKGROUND	109:118	arg1	vulgare					136:142	Hordeum vulgare L.	128:145	Hordeum vulgare L.	128:145	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	1	34	theme	BACKGROUND	109:118	arg1	grain					161:165	a healthy grain	151:165	a healthy grain	151:165	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	9	35	theme	thermal	1372:1378	arg1	stress					1380:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	4	36	theme	stresses	541:548	arg1	effect					517:522	the effect	513:522	the effect of environmental stresses on the quantity and quality of bioactive compounds	513:599	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	11	37	theme	late	1718:1721	arg1	genotypes					1733:1741	late flowering genotypes	1718:1741	late flowering genotypes	1718:1741	Late sowings or late flowering genotypes could also be considered, should a premium be implemented to compensate for lower yields.					
34000070	10	38	theme	heat	1689:1692	arg1	stress					1694:1699	heat stress	1689:1699	heat stress	1689:1699	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	0	39	theme	differential	37:48	arg1	accumulation					50:61	differential accumulation	37:61	differential accumulation of bioactive compounds in field-grown barley	37:106	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	5	40	dep	RESULTS	602:608	arg1	studied					618:624	studied	618:624	have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes	613:823	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	10	41	from	cultivation	1651:1661	arg1	areas					1674:1678	growing areas	1666:1678	growing areas prone to heat stress	1666:1699	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	9	42	theme	significant	1393:1403	arg1	effect					1405:1410	a significant effect	1391:1410	a significant effect	1391:1410	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	1	43	theme	compounds	225:233	arg1	content					187:193	its high content	178:193	its high content of dietary fibre and phenolic compounds	178:233	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	4	44	theme	compounds	591:599	arg1	quality					570:576	quality	570:576	quality	570:576	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	4	44	theme	compounds	591:599	arg1	quantity					557:564	quantity	557:564	quantity	557:564	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	12	45	dep	Authors	1844:1850	arg1	2021					1835:1838	© 2021	1833:1838	© 2021	1833:1838	© 2021 The Authors.					
34000070	9	46	contain	had	1387:1389	arg2	effect					1405:1410	a significant effect	1391:1410	a significant effect	1391:1410	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	9	46	contain	had	1387:1389	arg1	stress					1380:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	6	47	theme	compounds	922:930	arg1	composition					897:907	the relative composition	884:907	the relative composition of bioactive compounds	884:930	Thermal stress affected grain weight and size and changed the relative composition of bioactive compounds.					
34000070	10	48	theme	bioactive	1602:1610	arg1	concentrations					1612:1625	the bioactive concentrations	1598:1625	the bioactive concentrations in the barley grain	1598:1645	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	2	49	theme	grain	280:284	arg1	filling					286:292	grain filling	280:292	grain filling	280:292	It faces periods of high temperature during grain filling, frequently reducing grain weight.					
34000070	9	50	theme	compound	1450:1457	arg1	concentration					1459:1471	phenolic compound concentration	1441:1471	phenolic compound concentration	1441:1471	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	8	51	theme	direct	1166:1171	arg1	impact					1173:1178	a significant direct impact	1152:1178	a significant direct impact	1152:1178	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	13	52	theme	Chemical	1959:1966	arg1	Industry					1968:1975	Chemical Industry	1959:1975	Chemical Industry	1959:1975	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
34000070	2	53	theme	temperature	261:271	arg1	periods					245:251	periods	245:251	periods of high temperature	245:271	It faces periods of high temperature during grain filling, frequently reducing grain weight.					
34000070	6	54	theme	relative	888:895	arg1	composition					897:907	the relative composition	884:907	the relative composition of bioactive compounds	884:930	Thermal stress affected grain weight and size and changed the relative composition of bioactive compounds.					
34000070	10	55	theme	barley	1634:1639	arg1	grain					1641:1645	the barley grain	1630:1645	the barley grain	1630:1645	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	5	56	theme	consecutive	772:782	arg1	seasons					792:798	two consecutive growing seasons	768:798	two consecutive growing seasons in four barley genotypes	768:823	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	7	57	theme	grain	1097:1101	arg1	weight					1103:1108	the lower grain weight	1087:1108	the lower grain weight under stress	1087:1121	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	8	58	theme	phenolic	1188:1195	arg1	compounds					1197:1205	some phenolic compounds	1183:1205	some phenolic compounds	1183:1205	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	11	59	theme	lower	1819:1823	arg1	yields					1825:1830	lower yields	1819:1830	lower yields	1819:1830	Late sowings or late flowering genotypes could also be considered, should a premium be implemented to compensate for lower yields.					
34000070	5	60	theme	bioactive	679:687	arg1	compounds					689:697	barley bioactive compounds	672:697	barley bioactive compounds	672:697	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	9	61	theme	barley	1501:1506	arg1	grains					1508:1513	barley grains	1501:1513	barley grains	1501:1513	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	3	62	attach	present	389:395	arg1	grain					404:408	the grain	400:408	the grain	400:408	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	3	62	attach	present	389:395	arg2	compounds					379:387	the bioactive compounds	365:387	the bioactive compounds present in the grain	365:408	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	5	63	theme	thermal	654:660	arg1	stress					662:667	post-anthesis thermal stress	640:667	post-anthesis thermal stress	640:667	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	7	64	theme	resulting	1037:1045	arg1	increases					1047:1055	the resulting increases	1033:1055	the resulting increases in concentrations	1033:1073	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	7	64	theme	resulting	1037:1045	arg1	due					1080:1082	due	1080:1082	due	1080:1082	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	1	65	theme	Hordeum	128:134	arg1	Barley					120:125	BACKGROUND Barley	109:125	BACKGROUND Barley (Hordeum vulgare L.)	109:146	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	1	65	theme	Hordeum	128:134	arg1	vulgare					136:142	Hordeum vulgare L.	128:145	Hordeum vulgare L.	128:145	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	8	66	theme	antioxidant	1312:1322	arg1	capacity					1324:1331	antioxidant capacity	1312:1331	antioxidant capacity of up to 30%	1312:1344	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	13	67	theme	Wiley	1918:1922	arg1	Sons					1926:1929	John Wiley & Sons Ltd	1913:1933	John Wiley & Sons Ltd on behalf of Society of Chemical Industry	1913:1975	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
34000070	8	68	from	improvement	1297:1307	arg1	capacity					1324:1331	antioxidant capacity	1312:1331	antioxidant capacity of up to 30%	1312:1344	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	3	69	theme	Heat	329:332	arg1	stress					334:339	Heat stress	329:339	Heat stress	329:339	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	4	70	theme	bioactive	581:589	arg1	compounds					591:599	bioactive compounds	581:599	bioactive compounds	581:599	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	3	71	from	present	389:395	arg1	grain					404:408	the grain	400:408	the grain	400:408	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	1	72	theme	healthy	153:159	arg1	Barley					120:125	BACKGROUND Barley	109:125	BACKGROUND Barley (Hordeum vulgare L.)	109:146	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	1	72	theme	healthy	153:159	arg1	grain					161:165	a healthy grain	151:165	a healthy grain	151:165	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	8	73	theme	%	1344:1344	arg1	capacity					1324:1331	antioxidant capacity	1312:1331	antioxidant capacity of up to 30%	1312:1344	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	5	74	from	seasons	792:798	arg1	genotypes					815:823	four barley genotypes	803:823	four barley genotypes	803:823	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	5	75	theme	Mediterranean	730:742	arg1	conditions					750:759	Mediterranean field conditions	730:759	Mediterranean field conditions	730:759	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	7	76	theme	grain	974:978	arg1	β-glucans					980:988	grain β-glucans	974:988	grain β-glucans	974:988	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	0	77	from	accumulation	50:61	arg1	barley					101:106	field-grown barley	89:106	field-grown barley	89:106	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	1	78	dep	vulgare	136:142	arg1	L.					144:145	Hordeum vulgare L.	128:145	Hordeum vulgare L.	128:145	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	13	79	theme	Science	1868:1874	arg1	Journal					1853:1859	Journal	1853:1859	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.	1853:1976	Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
34000070	0	80	theme	thermal	14:20	arg1	stress					22:27	Post-anthesis thermal stress	0:27	Post-anthesis thermal stress	0:27	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	3	81	theme	bioactive	369:377	arg1	compounds					379:387	the bioactive compounds	365:387	the bioactive compounds present in the grain	365:408	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	4	82	theme	environmental	527:539	arg1	stresses					541:548	environmental stresses	527:548	environmental stresses	527:548	To produce quality grains that provide nutritional and health benefits, it is important to understand the effect of environmental stresses on the quantity and quality of bioactive compounds.					
34000070	9	83	theme	Post-anthesis	1358:1370	arg1	stress					1380:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress	1347:1385	CONCLUSION Post-anthesis thermal stress had a significant effect on β-glucans, arabinoxylans, phenolic compound concentration and antioxidant capacity of barley grains.					
34000070	5	84	theme	growing	784:790	arg1	seasons					792:798	two consecutive growing seasons	768:798	two consecutive growing seasons in four barley genotypes	768:823	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	10	85	theme	grain	1522:1526	arg1	quality					1528:1534	Final grain quality	1516:1534	Final grain quality	1516:1534	Final grain quality could, at least partially, be controlled in order to increase the bioactive concentrations in the barley grain, by cultivation in growing areas prone to heat stress.					
34000070	11	86	theme	Late	1702:1705	arg1	sowings					1707:1713	Late sowings	1702:1713	Late sowings	1702:1713	Late sowings or late flowering genotypes could also be considered, should a premium be implemented to compensate for lower yields.					
34000070	0	87	theme	bioactive	66:74	arg1	compounds					76:84	bioactive compounds	66:84	bioactive compounds	66:84	Post-anthesis thermal stress induces differential accumulation of bioactive compounds in field-grown barley.					
34000070	8	88	dep	30	1342:1343	arg1	to					1339:1340	to	1339:1340	to	1339:1340	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	3	89	theme	present	389:395	arg1	compounds					379:387	the bioactive compounds	365:387	the bioactive compounds present in the grain	365:408	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	5	90	from	effect	630:635	arg1	compounds					689:697	barley bioactive compounds	672:697	barley bioactive compounds	672:697	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	5	90	from	effect	630:635	arg1	capacity					715:722	antioxidant capacity	703:722	antioxidant capacity	703:722	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34000070	8	91	theme	heat	1136:1139	arg1	stress					1141:1146	heat stress	1136:1146	heat stress	1136:1146	Conversely, heat stress had a significant direct impact on some phenolic compounds, increasing their concentrations differentially across genotypes, which contributed to an improvement in antioxidant capacity of up to 30%.					
34000070	6	92	theme	grain	850:854	arg1	weight					856:861	grain weight	850:861	grain weight	850:861	Thermal stress affected grain weight and size and changed the relative composition of bioactive compounds.					
34000070	12	93	theme	©	1833:1833	arg1	2021					1835:1838	© 2021	1833:1838	© 2021	1833:1838	© 2021 The Authors.					
34000070	7	94	from	increases	1047:1055	arg1	concentrations					1060:1073	concentrations	1060:1073	concentrations	1060:1073	The relationship between heat stress and grain β-glucans and arabinoxylans content was indirect, as the resulting increases in concentrations were due to the lower grain weight under stress.					
34000070	11	95	theme	flowering	1723:1731	arg1	genotypes					1733:1741	late flowering genotypes	1718:1741	late flowering genotypes	1718:1741	Late sowings or late flowering genotypes could also be considered, should a premium be implemented to compensate for lower yields.					
34000070	3	96	from	grain	404:408	arg1	present					389:395	present	389:395	present	389:395	Heat stress may also affect some of the bioactive compounds present in the grain.					
34000070	2	97	theme	grain	315:319	arg1	weight					321:326	grain weight	315:326	grain weight	315:326	It faces periods of high temperature during grain filling, frequently reducing grain weight.					
34000070	1	98	theme	phenolic	216:223	arg1	compounds					225:233	phenolic compounds	216:233	phenolic compounds	216:233	BACKGROUND Barley (Hordeum vulgare L.) is a healthy grain because of its high content of dietary fibre and phenolic compounds.					
34000070	5	99	theme	barley	808:813	arg1	genotypes					815:823	four barley genotypes	803:823	four barley genotypes	803:823	RESULTS We have studied the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity under Mediterranean field conditions during two consecutive growing seasons in four barley genotypes.					
34044932	6	0	theme	matrix	987:992	arg1	properties					1005:1014	the matrix mechanical properties	983:1014	the matrix mechanical properties	983:1014	Finally, a radiation dose of 25 Gy eliminated all F98 cells trapped in the matrix, without significantly altering the matrix mechanical properties.					
34044932	6	1	theme	25 Gy	898:902	arg1	dose					890:893	a radiation dose	878:893	a radiation dose of 25 Gy	878:902	Finally, a radiation dose of 25 Gy eliminated all F98 cells trapped in the matrix, without significantly altering the matrix mechanical properties.					
34044932	6	2	theme	mechanical	994:1003	arg1	properties					1005:1014	the matrix mechanical properties	983:1014	the matrix mechanical properties	983:1014	Finally, a radiation dose of 25 Gy eliminated all F98 cells trapped in the matrix, without significantly altering the matrix mechanical properties.					
34044932	5	3	dep	10	733:734	arg1	to					730:731	to	730:731	to	730:731	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	5	4	theme	associated	801:810	arg1	αvβ3					812:815	the associated αvβ3 and αvβ5 binding integrins	797:842	αvβ3	812:815	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	1	5	theme	lethal	218:223	arg1	dose					235:238	a lethal radiation dose	216:238	a lethal radiation dose	216:238	To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely.					
34044932	5	6	theme	RGD	671:673	arg1	peptides					689:696	RGD cell-adhesion peptides	671:696	RGD cell-adhesion peptides	671:696	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	5	7	from	increase	742:749	arg1	number					758:763	the number	754:763	the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins)	754:843	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	6	8	theme	radiation	880:888	arg1	dose					890:893	a radiation dose	878:893	a radiation dose of 25 Gy	878:902	Finally, a radiation dose of 25 Gy eliminated all F98 cells trapped in the matrix, without significantly altering the matrix mechanical properties.					
34044932	1	9	theme	radiation	225:233	arg1	dose					235:238	a lethal radiation dose	216:238	a lethal radiation dose	216:238	To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely.					
34044932	5	10	theme	cell-adhesion	675:687	arg1	peptides					689:696	RGD cell-adhesion peptides	671:696	RGD cell-adhesion peptides	671:696	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	5	11	theme	αvβ5	821:824	arg1	integrins					834:842	the associated αvβ3 and αvβ5 binding integrins	797:842	integrins	834:842	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	3	12	theme	hydrogel	432:439	arg1	matrices					441:448	the hydrogel matrices	428:448	the hydrogel matrices	428:448	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	3	13	theme	X-ray	400:404	arg1	tomography					406:415	Microcomputed X-ray tomography	386:415	Microcomputed X-ray tomography	386:415	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	5	14	theme	peptides	689:696	arg1	grafting					659:666	the grafting	655:666	the grafting of RGD cell-adhesion peptides to the alginate	655:712	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	3	15	theme	300 μm	508:513	arg1	diameter					496:503	an average diameter	485:503	an average diameter of 300 μm	485:513	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	0	16	theme	alginate-based	3:16	arg1	matrix					39:44	An alginate-based macroporous hydrogel matrix	0:44	An alginate-based macroporous hydrogel matrix	0:44	An alginate-based macroporous hydrogel matrix to trap cancer cells.					
34044932	4	17	theme	matrix	600:605	arg1	center					586:591	the center	582:591	the center of the matrix	582:605	The F98 GBM cells migrated in the pores and mainly accumulated in the center of the matrix.					
34044932	5	18	theme	times	736:740	arg1	increase					742:749	a 4 to 10 times increase	726:749	a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix	726:866	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	4	19	theme	GBM	524:526	arg1	cells					528:532	The F98 GBM cells	516:532	The F98 GBM cells	516:532	The F98 GBM cells migrated in the pores and mainly accumulated in the center of the matrix.					
34044932	3	20	with	pores	474:478	arg1	diameter					496:503	an average diameter	485:503	an average diameter of 300 μm	485:513	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	3	21	theme	Microcomputed	386:398	arg1	tomography					406:415	Microcomputed X-ray tomography	386:415	Microcomputed X-ray tomography	386:415	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	0	22	theme	hydrogel	30:37	arg1	matrix					39:44	An alginate-based macroporous hydrogel matrix	0:44	An alginate-based macroporous hydrogel matrix	0:44	An alginate-based macroporous hydrogel matrix to trap cancer cells.					
34044932	4	23	theme	F98	520:522	arg1	cells					528:532	The F98 GBM cells	516:532	The F98 GBM cells	516:532	The F98 GBM cells migrated in the pores and mainly accumulated in the center of the matrix.					
34044932	0	24	theme	macroporous	18:28	arg1	matrix					39:44	An alginate-based macroporous hydrogel matrix	0:44	An alginate-based macroporous hydrogel matrix	0:44	An alginate-based macroporous hydrogel matrix to trap cancer cells.					
34044932	2	25	theme	alginate-based	317:330	arg1	hydrogel					344:351	a sodium alginate-based macroporous hydrogel	308:351	a sodium alginate-based macroporous hydrogel	308:351	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	25	theme	alginate-based	317:330	arg1	trap					380:383	a potential cancer cell trap	356:383	a potential cancer cell trap	356:383	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	1	26	theme	glioblastoma	103:114	arg1	cells					122:126	glioblastoma (GBM) cells	103:126	glioblastoma (GBM) cells infiltrated in the brain	103:151	To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely.					
34044932	2	27	theme	cell	375:378	arg1	hydrogel					344:351	a sodium alginate-based macroporous hydrogel	308:351	a sodium alginate-based macroporous hydrogel	308:351	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	27	theme	cell	375:378	arg1	trap					380:383	a potential cancer cell trap	356:383	a potential cancer cell trap	356:383	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	5	28	theme	binding	826:832	arg1	integrins					834:842	the associated αvβ3 and αvβ5 binding integrins	797:842	integrins	834:842	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	1	29	theme	cancer	182:187	arg1	cells					189:193	these cancer cells	176:193	these cancer cells	176:193	To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely.					
34044932	2	30	theme	macroporous	332:342	arg1	hydrogel					344:351	a sodium alginate-based macroporous hydrogel	308:351	a sodium alginate-based macroporous hydrogel	308:351	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	30	theme	macroporous	332:342	arg1	trap					380:383	a potential cancer cell trap	356:383	a potential cancer cell trap	356:383	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	31	theme	cancer	368:373	arg1	hydrogel					344:351	a sodium alginate-based macroporous hydrogel	308:351	a sodium alginate-based macroporous hydrogel	308:351	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	31	theme	cancer	368:373	arg1	trap					380:383	a potential cancer cell trap	356:383	a potential cancer cell trap	356:383	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	5	32	theme	cells	772:776	arg1	number					758:763	the number	754:763	the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins)	754:843	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	2	33	theme	potential	358:366	arg1	hydrogel					344:351	a sodium alginate-based macroporous hydrogel	308:351	a sodium alginate-based macroporous hydrogel	308:351	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	33	theme	potential	358:366	arg1	trap					380:383	a potential cancer cell trap	356:383	a potential cancer cell trap	356:383	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	0	34	theme	cancer	54:59	arg1	cells					61:65	cancer cells	54:65	cancer cells	54:65	An alginate-based macroporous hydrogel matrix to trap cancer cells.					
34044932	3	35	theme	interconnected	459:472	arg1	pores					474:478	interconnected pores	459:478	interconnected pores with an average diameter of 300 μm	459:513	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	1	36	theme	GBM	117:119	arg1	cells					122:126	glioblastoma (GBM) cells	103:126	glioblastoma (GBM) cells infiltrated in the brain	103:151	To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely.					
34044932	5	37	theme	cancer	635:640	arg1	cells					642:646	cancer cells	635:646	cancer cells added	635:652	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	5	38	theme	cells	642:646	arg1	number					625:630	the number	621:630	the number of cancer cells added	621:652	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	1	39	theme	cells	122:126	arg1	radioresistance					84:98	the radioresistance	80:98	the radioresistance of glioblastoma (GBM) cells infiltrated in the brain	80:151	To overcome the radioresistance of glioblastoma (GBM) cells infiltrated in the brain, we propose to attract these cancer cells into a trap to which a lethal radiation dose can be delivered safely.					
34044932	6	40	theme	F98	919:921	arg1	cells					923:927	all F98 cells	915:927	all F98 cells trapped in the matrix	915:949	Finally, a radiation dose of 25 Gy eliminated all F98 cells trapped in the matrix, without significantly altering the matrix mechanical properties.					
34044932	5	41	theme	F98	768:770	arg1	cells					772:776	F98 cells	768:776	F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins)	768:843	Depending on the number of cancer cells added, the grafting of RGD cell-adhesion peptides to the alginate resulted in a 4 to 10 times increase in the number of F98 cells (which overexpress the associated αvβ3 and αvβ5 binding integrins) retained in the matrix.					
34044932	3	42	theme	average	488:494	arg1	diameter					496:503	an average diameter	485:503	an average diameter of 300 μm	485:513	Microcomputed X-ray tomography shows that the hydrogel matrices comprise interconnected pores with an average diameter of 300 μm.					
34044932	2	43	theme	sodium	310:315	arg1	hydrogel					344:351	a sodium alginate-based macroporous hydrogel	308:351	a sodium alginate-based macroporous hydrogel	308:351	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
34044932	2	43	theme	sodium	310:315	arg1	trap					380:383	a potential cancer cell trap	356:383	a potential cancer cell trap	356:383	Herein, we have prepared and characterized a sodium alginate-based macroporous hydrogel as a potential cancer cell trap.					
33668338	8	0	theme	color	1612:1616	arg1	variation					1618:1626	the largest color variation	1600:1626	the largest color variation	1600:1626	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	5	1	theme	UV	1162:1163	arg1	irradiation					1171:1181	UV light irradiation	1162:1181	UV light irradiation	1162:1181	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	10	2	theme	degradation	1967:1977	arg1	increase					1942:1949	the increase	1938:1949	the increase of the material degradation in the presence of TiO2 NPs	1938:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	3	3	theme	protective	678:687	arg1	properties					689:698	the packaging food protective properties	659:698	the packaging food protective properties	659:698	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	6	4	theme	color	1237:1241	arg1	changes					1243:1249	the systems color changes	1225:1249	the systems color changes (CIELAB)	1225:1258	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	6	4	theme	color	1237:1241	arg1	CIELAB					1252:1257	CIELAB	1252:1257	CIELAB	1252:1257	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	10	5	theme	NPs	2003:2005	arg1	presence					1986:1993	the presence	1982:1993	the presence of TiO2 NPs	1982:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	3	6	theme	packaging	663:671	arg1	properties					689:698	the packaging food protective properties	659:698	the packaging food protective properties	659:698	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	1	7	theme	excellent	255:263	arg1	properties					265:274	their excellent properties	249:274	their excellent properties	249:274	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	10	8	theme	FTIR	1855:1858	arg1	spectroscopy					1860:1871	FTIR spectroscopy	1855:1871	FTIR spectroscopy	1855:1871	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	10	9	from	variation	1877:1885	arg1	intensities					1901:1911	the signal intensities	1890:1911	the signal intensities	1890:1911	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	3	10	theme	nanoparticles	530:542	arg1	types					521:525	These types	515:525	These types of nanoparticles	515:542	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	8	11	theme	nanocomposite	1648:1660	arg1	films					1662:1666	the nanocomposite films	1644:1666	the nanocomposite films	1644:1666	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	5	12	theme	photocatalytic	825:838	arg1	activity					840:847	the photocatalytic activity	821:847	the photocatalytic activity of the nanoparticles	821:868	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	6	13	dep	transform	1272:1280	arg1	infrared					1282:1289	infrared	1282:1289	transform infrared spectroscopy (FTIR) spectra	1272:1317	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	2	14	theme	nanocomposite	488:500	arg1	degradation					502:512	nanocomposite degradation	488:512	nanocomposite degradation	488:512	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	4	15	contain	containing	721:730	arg2	PVA					784:786	PVA	784:786	PVA	784:786	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	4	15	contain	containing	721:730	arg1	films					715:719	films	715:719	films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH	715:794	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	4	15	contain	containing	721:730	arg2	nanoparticles					766:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	1	16	used	used	191:194	arg2	copolymers					162:171	ethylene-PVA copolymers	149:171	ethylene-PVA copolymers (EVOH)	149:178	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	16	used	used	191:194	arg2	materials					102:110	Polymeric materials	92:110	Polymeric materials	92:110	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	16	used	used	191:194	arg2	alcohol					131:137	polyvinyl alcohol	121:137	polyvinyl alcohol (PVA)	121:143	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	16	used	used	191:194	arg2	materials					228:236	packaging materials	218:236	packaging materials because of their excellent properties	218:274	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	5	17	theme	nanoparticle	1004:1015	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	18	theme	NP	1018:1019	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	19	theme	environmental	887:899	arg1	protection					901:910	the possible environmental protection	874:910	the possible environmental protection	874:910	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	7	20	theme	CDs	1522:1524	arg1	presence					1510:1517	the presence	1506:1517	the presence of CDs	1506:1524	There was no significant influence of the presence of CDs during the degradation process.					
33668338	2	21	theme	other	459:463	arg1	hand					465:468	the other hand	455:468	the other hand	455:468	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	9	22	theme	photocatalytic	1781:1794	arg1	activity					1796:1803	the high photocatalytic activity	1772:1803	the high photocatalytic activity of TiO2	1772:1811	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	10	23	theme	signal	1894:1899	arg1	intensities					1901:1911	the signal intensities	1890:1911	the signal intensities	1890:1911	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	0	24	theme	TiO2	72:75	arg1	Nanoparticles					77:89	β-Cyclodextrin-Grafted TiO2 Nanoparticles	49:89	β-Cyclodextrin-Grafted TiO2 Nanoparticles	49:89	Aging Studies on Food Packaging Films Containing β-Cyclodextrin-Grafted TiO2 Nanoparticles.					
33668338	7	25	theme	presence	1510:1517	arg1	influence					1493:1501	no significant influence	1478:1501	no significant influence of the presence of CDs	1478:1524	There was no significant influence of the presence of CDs during the degradation process.					
33668338	5	26	theme	chamber	1077:1083	arg1	methods					1050:1056	two methods	1046:1056	two methods	1046:1056	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	26	theme	chamber	1077:1083	arg1	experiments					1085:1095	stability chamber experiments	1067:1095	stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities	1067:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	27	theme	nanoparticles	856:868	arg1	activity					840:847	the photocatalytic activity	821:847	the photocatalytic activity of the nanoparticles	821:868	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	27	theme	nanoparticles	856:868	arg1	protection					901:910	the possible environmental protection	874:910	the possible environmental protection	874:910	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	7	28	theme	significant	1481:1491	arg1	influence					1493:1501	no significant influence	1478:1501	no significant influence of the presence of CDs	1478:1524	There was no significant influence of the presence of CDs during the degradation process.					
33668338	9	29	theme	color	1696:1700	arg1	relevant					1718:1725	relevant	1718:1725	relevant	1718:1725	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	9	29	theme	color	1696:1700	arg1	change					1702:1707	the color change	1692:1707	the color change	1692:1707	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	4	30	theme	β-Cyclodextrin	732:745	arg1	nanoparticles					766:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	1	31	theme	Polymeric	92:100	arg1	copolymers					162:171	ethylene-PVA copolymers	149:171	ethylene-PVA copolymers (EVOH)	149:178	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	31	theme	Polymeric	92:100	arg1	materials					228:236	packaging materials	218:236	packaging materials because of their excellent properties	218:274	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	31	theme	Polymeric	92:100	arg1	materials					102:110	Polymeric materials	92:110	Polymeric materials	92:110	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	31	theme	Polymeric	92:100	arg1	alcohol					131:137	polyvinyl alcohol	121:137	polyvinyl alcohol (PVA)	121:143	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	0	32	theme	Aging	0:4	arg1	Studies					6:12	Aging Studies	0:12	Aging Studies on Food Packaging Films	0:36	Aging Studies on Food Packaging Films Containing β-Cyclodextrin-Grafted TiO2 Nanoparticles.					
33668338	5	33	theme	temperature	1124:1134	arg1	conditions					1110:1119	different conditions	1100:1119	different conditions of temperature and relative humidity and UV light irradiation at different intensities	1100:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	2	34	dep	expected	401:408	arg1	whereas					444:450	whereas	444:450	whereas	444:450	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	10	35	from	presence	1986:1993	arg1	increase					1942:1949	the increase	1938:1949	the increase of the material degradation in the presence of TiO2 NPs	1938:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	5	36	theme	relative	1140:1147	arg1	humidity					1149:1156	relative humidity	1140:1156	relative humidity	1140:1156	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	37	theme	aging	925:929	arg1	tests					931:935	accelerated aging tests	913:935	accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films	913:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	0	38	theme	Packaging	22:30	arg1	Films					32:36	Food Packaging Films	17:36	Food Packaging Films	17:36	Aging Studies on Food Packaging Films Containing β-Cyclodextrin-Grafted TiO2 Nanoparticles.					
33668338	2	39	theme	bactericidal	421:432	arg1	capacity					434:441	bactericidal capacity	421:441	bactericidal capacity	421:441	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	6	40	theme	film	1345:1348	arg1	degradation					1350:1360	the film degradation	1341:1360	the film degradation	1341:1360	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	5	41	theme	TiO2	999:1002	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	10	42	theme	material	1958:1965	arg1	degradation					1967:1977	the material degradation	1954:1977	the material degradation in the presence of TiO2 NPs	1954:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	3	43	theme	preservative	625:636	arg1	macromolecules					581:594	cyclodextrin macromolecules	568:594	cyclodextrin macromolecules (CDs)	568:600	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	3	43	theme	preservative	625:636	arg1	carriers					638:645	food preservative carriers	620:645	food preservative carriers	620:645	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	4	44	theme	βCD	748:750	arg1	nanoparticles					766:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	2	45	theme	photocatalytic	307:320	arg1	activity					322:329	photocatalytic activity	307:329	photocatalytic activity	307:329	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	6	46	theme	relative	1429:1436	arg1	humidity					1438:1445	relative humidity	1429:1445	relative humidity percentage (28-80%)	1429:1465	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	1	47	theme	food	203:206	arg1	sector					208:213	the food sector	199:213	the food sector	199:213	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	10	48	theme	TiO2	1998:2001	arg1	NPs					2003:2005	TiO2 NPs	1998:2005	TiO2 NPs	1998:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	4	49	theme	-grafted	752:759	arg1	nanoparticles					766:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	8	50	theme	UV	1590:1591	arg1	light					1593:1597	UV light	1590:1597	UV light	1590:1597	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	5	51	with	EVOH	946:949	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	52	from	conditions	1110:1119	arg1	intensities					1196:1206	different intensities	1186:1206	different intensities	1186:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	52	from	conditions	1110:1119	arg1	methods					1050:1056	two methods	1046:1056	two methods	1046:1056	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	52	from	conditions	1110:1119	arg1	experiments					1085:1095	stability chamber experiments	1067:1095	stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities	1067:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	8	53	located	observed	1632:1639	arg2	variation					1618:1626	the largest color variation	1600:1626	the largest color variation	1600:1626	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	8	53	located	observed	1632:1639	arg1	films					1662:1666	the nanocomposite films	1644:1666	the nanocomposite films	1644:1666	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	9	54	theme	high	1776:1779	arg1	activity					1796:1803	the high photocatalytic activity	1772:1803	the high photocatalytic activity of TiO2	1772:1811	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	2	55	theme	TiO2	277:280	arg1	NPs					297:299	NPs	297:299	NPs	297:299	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	2	55	theme	TiO2	277:280	arg1	nanoparticles					282:294	TiO2 nanoparticles	277:294	TiO2 nanoparticles (NPs)	277:300	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	8	56	theme	largest	1604:1610	arg1	variation					1618:1626	the largest color variation	1600:1626	the largest color variation	1600:1626	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	5	57	theme	light	1165:1169	arg1	irradiation					1171:1181	UV light irradiation	1162:1181	UV light irradiation	1162:1181	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	58	with	composites	962:971	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	6	59	theme	systems	1229:1235	arg1	changes					1243:1249	the systems color changes	1225:1249	the systems color changes (CIELAB)	1225:1258	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	6	59	theme	systems	1229:1235	arg1	CIELAB					1252:1257	CIELAB	1252:1257	CIELAB	1252:1257	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	9	60	theme	TiO2	1808:1811	arg1	activity					1796:1803	the high photocatalytic activity	1772:1803	the high photocatalytic activity of TiO2	1772:1811	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	3	61	theme	food	673:676	arg1	properties					689:698	the packaging food protective properties	659:698	the packaging food protective properties	659:698	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	6	62	theme	FTIR	1305:1308	arg1	spectra					1311:1317	spectroscopy (FTIR) spectra	1291:1317	spectroscopy (FTIR) spectra	1291:1317	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	6	63	dep	Fourier	1264:1270	arg1	transform					1272:1280	transform	1272:1280	transform infrared spectroscopy (FTIR) spectra	1272:1317	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	5	64	with	PVA	941:943	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	6	65	theme	spectroscopy	1291:1302	arg1	spectra					1311:1317	spectroscopy (FTIR) spectra	1291:1317	spectroscopy (FTIR) spectra	1291:1317	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	7	66	theme	degradation	1537:1547	arg1	process					1549:1555	the degradation process	1533:1555	the degradation process	1533:1555	There was no significant influence of the presence of CDs during the degradation process.					
33668338	5	67	from	intensities	1196:1206	arg1	conditions					1110:1119	different conditions	1100:1119	different conditions of temperature and relative humidity and UV light irradiation at different intensities	1100:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	67	from	intensities	1196:1206	arg1	irradiation					1171:1181	UV light irradiation	1162:1181	UV light irradiation	1162:1181	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	67	from	intensities	1196:1206	arg1	temperature					1124:1134	temperature	1124:1134	temperature	1124:1134	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	67	from	intensities	1196:1206	arg1	humidity					1149:1156	relative humidity	1140:1156	relative humidity	1140:1156	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	1	68	theme	polyvinyl	121:129	arg1	PVA					140:142	PVA	140:142	PVA	140:142	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	68	theme	polyvinyl	121:129	arg1	alcohol					131:137	polyvinyl alcohol	121:137	polyvinyl alcohol (PVA)	121:143	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	5	69	theme	possible	878:885	arg1	protection					901:910	the possible environmental protection	874:910	the possible environmental protection	874:910	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	3	70	theme	cyclodextrin	568:579	arg1	macromolecules					581:594	cyclodextrin macromolecules	568:594	cyclodextrin macromolecules (CDs)	568:600	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	3	70	theme	cyclodextrin	568:579	arg1	carriers					638:645	food preservative carriers	620:645	food preservative carriers	620:645	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	3	70	theme	cyclodextrin	568:579	arg1	CDs					597:599	CDs	597:599	CDs	597:599	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	5	71	theme	stability	1067:1075	arg1	methods					1050:1056	two methods	1046:1056	two methods	1046:1056	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	71	theme	stability	1067:1075	arg1	experiments					1085:1095	stability chamber experiments	1067:1095	stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities	1067:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	1	72	theme	ethylene-PVA	149:160	arg1	EVOH					174:177	EVOH	174:177	EVOH	174:177	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	72	theme	ethylene-PVA	149:160	arg1	copolymers					162:171	ethylene-PVA copolymers	149:171	ethylene-PVA copolymers (EVOH)	149:178	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	0	73	from	Studies	6:12	arg1	Films					32:36	Food Packaging Films	17:36	Food Packaging Films	17:36	Aging Studies on Food Packaging Films Containing β-Cyclodextrin-Grafted TiO2 Nanoparticles.					
33668338	10	74	from	increase	1942:1949	arg1	presence					1986:1993	the presence	1982:1993	the presence of TiO2 NPs	1982:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	10	75	from	degradation	1967:1977	arg1	presence					1986:1993	the presence	1982:1993	the presence of TiO2 NPs	1982:2005	In addition, films were characterized by FTIR spectroscopy and variation in the signal intensities was observed, suggesting the increase of the material degradation in the presence of TiO2 NPs.					
33668338	2	76	theme	aforementioned	350:363	arg1	polymers					365:372	the aforementioned polymers	346:372	the aforementioned polymers	346:372	TiO2 nanoparticles (NPs) show photocatalytic activity; when added to the aforementioned polymers, on the one hand, they are expected to provide bactericidal capacity, whereas on the other hand, they could favor nanocomposite degradation.					
33668338	0	77	theme	Food	17:20	arg1	Films					32:36	Food Packaging Films	17:36	Food Packaging Films	17:36	Aging Studies on Food Packaging Films Containing β-Cyclodextrin-Grafted TiO2 Nanoparticles.					
33668338	1	78	theme	properties	265:274	arg1	copolymers					162:171	ethylene-PVA copolymers	149:171	ethylene-PVA copolymers (EVOH)	149:178	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	78	theme	properties	265:274	arg1	materials					102:110	Polymeric materials	92:110	Polymeric materials	92:110	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	78	theme	properties	265:274	arg1	materials					228:236	packaging materials	218:236	packaging materials because of their excellent properties	218:274	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	78	theme	properties	265:274	arg1	alcohol					131:137	polyvinyl alcohol	121:137	polyvinyl alcohol (PVA)	121:143	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	5	79	theme	accelerated	913:923	arg1	tests					931:935	accelerated aging tests	913:935	accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films	913:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	80	theme	humidity	1149:1156	arg1	conditions					1110:1119	different conditions	1100:1119	different conditions of temperature and relative humidity and UV light irradiation at different intensities	1100:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	9	81	theme	NP	1743:1744	arg1	percentages					1746:1756	NP percentages	1743:1756	NP percentages (1-5%)	1743:1763	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	9	81	theme	NP	1743:1744	arg1	%					1762:1762	1-5%	1759:1762	1-5%	1759:1762	Moreover, the color change was more relevant with increasing NP percentages (1-5%) due to the high photocatalytic activity of TiO2.					
33668338	8	82	with	films	1579:1583	arg1	light					1593:1597	UV light	1590:1597	UV light	1590:1597	When irradiating the films with UV light, the largest color variation was observed in the nanocomposite films, as expected.					
33668338	4	83	theme	TiO2	761:764	arg1	nanoparticles					766:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles	732:778	In this work, films containing β-Cyclodextrin (βCD)-grafted TiO2 nanoparticles and PVA or EVOH were prepared.					
33668338	0	84	theme	β-Cyclodextrin-Grafted	49:70	arg1	Nanoparticles					77:89	β-Cyclodextrin-Grafted TiO2 Nanoparticles	49:89	β-Cyclodextrin-Grafted TiO2 Nanoparticles	49:89	Aging Studies on Food Packaging Films Containing β-Cyclodextrin-Grafted TiO2 Nanoparticles.					
33668338	5	85	theme	different	1100:1108	arg1	conditions					1110:1119	different conditions	1100:1119	different conditions of temperature and relative humidity and UV light irradiation at different intensities	1100:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	5	86	theme	cyclodextrin-grafted	978:997	arg1	films					1022:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	cyclodextrin-grafted TiO2 nanoparticle (NP) films	978:1026	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	3	87	theme	food	620:623	arg1	macromolecules					581:594	cyclodextrin macromolecules	568:594	cyclodextrin macromolecules (CDs)	568:600	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	3	87	theme	food	620:623	arg1	carriers					638:645	food preservative carriers	620:645	food preservative carriers	620:645	These types of nanoparticles can be derivatized with cyclodextrin macromolecules (CDs), which can act as food preservative carriers, increasing the packaging food protective properties.					
33668338	6	88	theme	humidity	1438:1445	arg1	percentage					1447:1456	relative humidity percentage	1429:1456	relative humidity percentage (28-80%)	1429:1465	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	6	88	theme	humidity	1438:1445	arg1	%					1464:1464	28-80%	1459:1464	28-80%	1459:1464	After analyzing the systems color changes (CIELAB) and Fourier transform infrared spectroscopy (FTIR) spectra, it was observed that the film degradation became more evident when increasing the temperature (25-80 °C) and relative humidity percentage (28-80%).					
33668338	5	89	theme	different	1186:1194	arg1	intensities					1196:1206	different intensities	1186:1206	different intensities	1186:1206	Regarding the photocatalytic activity of the nanoparticles and the possible environmental protection, accelerated aging tests for PVA, EVOH, and their composites with cyclodextrin-grafted TiO2 nanoparticle (NP) films were performed by two methods, namely, stability chamber experiments at different conditions of temperature and relative humidity and UV light irradiation at different intensities.					
33668338	1	90	theme	packaging	218:226	arg1	copolymers					162:171	ethylene-PVA copolymers	149:171	ethylene-PVA copolymers (EVOH)	149:178	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	90	theme	packaging	218:226	arg1	materials					102:110	Polymeric materials	92:110	Polymeric materials	92:110	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	90	theme	packaging	218:226	arg1	materials					228:236	packaging materials	218:236	packaging materials because of their excellent properties	218:274	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33668338	1	90	theme	packaging	218:226	arg1	alcohol					131:137	polyvinyl alcohol	121:137	polyvinyl alcohol (PVA)	121:143	Polymeric materials, such as polyvinyl alcohol (PVA) and ethylene-PVA copolymers (EVOH) are widely used in the food sector as packaging materials because of their excellent properties.					
33864456	9	0	theme	fibres	1181:1186	arg1	Fermentation					1151:1162	Fermentation	1151:1162	Fermentation of other dietary fibres	1151:1186	Fermentation of other dietary fibres can be studied using the same approach.					
33864456	4	1	from	shift	701:705	arg1	dynamics					714:721	the dynamics	710:721	the dynamics of mucin degrading species	710:748	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	8	2	theme	pH	1077:1078	arg1	importance					1063:1072	the importance	1059:1072	the importance of pH in the development of faecal microbiota in pectin-supplemented medium	1059:1148	Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium.					
33864456	4	3	dep	Faecalibacterium	511:526	arg1	comes					544:548	comes	544:548	comes	544:548	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	2	4	theme	pooled	257:262	arg1	microbiota					271:280	pooled faecal microbiota	257:280	pooled faecal microbiota	257:280	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	5	theme	faecal	264:269	arg1	microbiota					271:280	pooled faecal microbiota	257:280	pooled faecal microbiota	257:280	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	6	dep	rates	334:338	arg1	Dhigh = 0.2					340:350	Dhigh = 0.2	340:350	Dhigh = 0.2	340:350	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	6	dep	rates	334:338	arg1	1/h					368:370	Dlow = 0.05 1/h	356:370	Dlow = 0.05 1/h	356:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	6	dep	rates	334:338	arg1	rates					334:338	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	7	from	effect	198:203	arg1	metabolism					243:252	metabolism	243:252	metabolism	243:252	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	7	from	effect	198:203	arg1	composition					227:237	composition	227:237	composition	227:237	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	4	8	theme	 while	587:592	arg1	abundance					598:606	 while the abundance	587:606	 while the abundance of Akkermansia muciniphila	587:633	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	2	9	theme	dilution	325:332	arg1	Dhigh = 0.2					340:350	Dhigh = 0.2	340:350	Dhigh = 0.2	340:350	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	9	theme	dilution	325:332	arg1	1/h					368:370	Dlow = 0.05 1/h	356:370	Dlow = 0.05 1/h	356:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	9	theme	dilution	325:332	arg1	rates					334:338	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	6	10	theme	more	815:818	arg1	CO2					820:822	Substantially more CO2	801:822	Substantially more CO2	801:822	Substantially more CO2 was produced at Dlow than at Dhigh (18-29 vs 12-23 mmol per L medium, respectively).					
33864456	2	11	dep	composition	227:237	arg1	the					223:225	the	223:225	the	223:225	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	3	12	theme	ovatus	429:434	arg1	adaptability					401:412	the best adaptability	392:412	the best adaptability of Bacteroides ovatus	392:434	The results showed the best adaptability of Bacteroides ovatus within the pH range 6.0-8.0 at both dilution rates.					
33864456	5	13	theme	Increased	751:759	arg1	formation					765:773	Increased gas formation	751:773	Increased gas formation	751:773	Increased gas formation was observed at pH < 6.5.					
33864456	6	14	theme	L	884:884	arg1	medium					886:891	L medium	884:891	L medium	884:891	Substantially more CO2 was produced at Dlow than at Dhigh (18-29 vs 12-23 mmol per L medium, respectively).					
33864456	1	15	theme	Environmental	73:85	arg1	rate					106:109	gut transit rate	94:109	gut transit rate	94:109	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	15	theme	Environmental	73:85	arg1	factors					123:129	the key factors	115:129	the key factors determining the dynamics of colonic microbiota	115:176	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	15	theme	Environmental	73:85	arg1	pH					87:88	Environmental pH	73:88	Environmental pH	73:88	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	10	16	theme	gut	1280:1282	arg1	research					1284:1291	gut research	1280:1291	gut research	1280:1291	The significance of pH should be more emphasized in gut research and diagnostics.					
33864456	1	17	theme	colonic	159:165	arg1	microbiota					167:176	colonic microbiota	159:176	colonic microbiota	159:176	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	9	18	theme	same	1213:1216	arg1	approach					1218:1225	the same approach	1209:1225	the same approach	1209:1225	Fermentation of other dietary fibres can be studied using the same approach.					
33864456	4	19	theme	species	742:748	arg1	dynamics					714:721	the dynamics	710:721	the dynamics of mucin degrading species	710:748	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	0	20	from	pectin	44:49	arg1	production					28:37	butyrate production	19:37	butyrate production from pectin	19:49	Acidic pH enhances butyrate production from pectin by faecal microbiota.					
33864456	1	21	theme	microbiota	167:176	arg1	dynamics					147:154	the dynamics	143:154	the dynamics of colonic microbiota	143:176	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	0	22	theme	Acidic	0:5	arg1	pH					7:8	Acidic pH	0:8	Acidic pH	0:8	Acidic pH enhances butyrate production from pectin by faecal microbiota.					
33864456	4	23	theme	degrading	732:740	arg1	species					742:748	mucin degrading species	726:748	mucin degrading species	726:748	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	3	24	theme	Bacteroides	417:427	arg1	ovatus					429:434	Bacteroides ovatus	417:434	Bacteroides ovatus	417:434	The results showed the best adaptability of Bacteroides ovatus within the pH range 6.0-8.0 at both dilution rates.					
33864456	0	25	theme	butyrate	19:26	arg1	production					28:37	butyrate production	19:37	butyrate production from pectin	19:49	Acidic pH enhances butyrate production from pectin by faecal microbiota.					
33864456	2	26	theme	microbiota	271:280	arg1	metabolism					243:252	metabolism	243:252	metabolism	243:252	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	26	theme	microbiota	271:280	arg1	composition					227:237	composition	227:237	composition	227:237	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	9	27	theme	other	1167:1171	arg1	fibres					1181:1186	other dietary fibres	1167:1186	other dietary fibres	1167:1186	Fermentation of other dietary fibres can be studied using the same approach.					
33864456	7	28	theme	increased	988:996	arg1	abundance					998:1006	the simultaneous increased abundance	971:1006	the simultaneous increased abundance of Methanobrevibacter smithii	971:1036	Methane was produced only at Dlow and pH > 7, consistent with the simultaneous increased abundance of Methanobrevibacter smithii.					
33864456	1	29	theme	transit	98:104	arg1	rate					106:109	gut transit rate	94:109	gut transit rate	94:109	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	29	theme	transit	98:104	arg1	pH					87:88	Environmental pH	73:88	Environmental pH	73:88	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	29	theme	transit	98:104	arg1	factors					123:129	the key factors	115:129	the key factors determining the dynamics of colonic microbiota	115:176	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	4	30	theme	pH-dependant	688:699	arg1	shift					701:705	a pH-dependant shift	686:705	a pH-dependant shift in the dynamics of mucin degrading species	686:748	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	3	31	theme	best	396:399	arg1	adaptability					401:412	the best adaptability	392:412	the best adaptability of Bacteroides ovatus	392:434	The results showed the best adaptability of Bacteroides ovatus within the pH range 6.0-8.0 at both dilution rates.					
33864456	6	32	theme	18-29 vs	860:867	arg1	12-23 mmol					869:878	18-29 vs 12-23 mmol	860:878	18-29 vs 12-23 mmol per L medium, respectively	860:905	Substantially more CO2 was produced at Dlow than at Dhigh (18-29 vs 12-23 mmol per L medium, respectively).					
33864456	4	33	theme	pH	662:663	arg1	Dhigh					671:675	pH >7 at Dhigh	662:675	pH >7 at Dhigh	662:675	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	3	34	theme	pH	447:448	arg1	range					450:454	the pH range 6.0-8.0	443:462	the pH range 6.0-8.0	443:462	The results showed the best adaptability of Bacteroides ovatus within the pH range 6.0-8.0 at both dilution rates.					
33864456	6	35	dep	Dhigh	853:857	arg1	12-23 mmol					869:878	18-29 vs 12-23 mmol	860:878	18-29 vs 12-23 mmol per L medium, respectively	860:905	Substantially more CO2 was produced at Dlow than at Dhigh (18-29 vs 12-23 mmol per L medium, respectively).					
33864456	8	36	theme	faecal	1102:1107	arg1	microbiota					1109:1118	faecal microbiota	1102:1118	faecal microbiota	1102:1118	Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium.					
33864456	8	37	theme	pectin-supplemented	1123:1141	arg1	medium					1143:1148	pectin-supplemented medium	1123:1148	pectin-supplemented medium	1123:1148	Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium.					
33864456	7	38	theme	Methanobrevibacter	1011:1028	arg1	abundance					998:1006	the simultaneous increased abundance	971:1006	the simultaneous increased abundance of Methanobrevibacter smithii	971:1036	Methane was produced only at Dlow and pH > 7, consistent with the simultaneous increased abundance of Methanobrevibacter smithii.					
33864456	3	39	theme	dilution	472:479	arg1	rates					481:485	both dilution rates	467:485	both dilution rates	467:485	The results showed the best adaptability of Bacteroides ovatus within the pH range 6.0-8.0 at both dilution rates.					
33864456	5	40	located	observed	779:786	arg2	formation					765:773	Increased gas formation	751:773	Increased gas formation	751:773	Increased gas formation was observed at pH < 6.5.					
33864456	5	40	located	observed	779:786	arg1	pH < 6.5					791:798	pH < 6.5	791:798	pH < 6.5	791:798	Increased gas formation was observed at pH < 6.5.					
33864456	2	41	theme	relevant	316:323	arg1	Dhigh = 0.2					340:350	Dhigh = 0.2	340:350	Dhigh = 0.2	340:350	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	41	theme	relevant	316:323	arg1	1/h					368:370	Dlow = 0.05 1/h	356:370	Dlow = 0.05 1/h	356:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	41	theme	relevant	316:323	arg1	rates					334:338	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	10	42	theme	pH	1248:1249	arg1	significance					1232:1243	The significance	1228:1243	The significance of pH	1228:1249	The significance of pH should be more emphasized in gut research and diagnostics.					
33864456	4	43	theme	mucin	726:730	arg1	species					742:748	mucin degrading species	726:748	mucin degrading species	726:748	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	1	44	theme	gut	94:96	arg1	rate					106:109	gut transit rate	94:109	gut transit rate	94:109	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	44	theme	gut	94:96	arg1	pH					87:88	Environmental pH	73:88	Environmental pH	73:88	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	44	theme	gut	94:96	arg1	factors					123:129	the key factors	115:129	the key factors determining the dynamics of colonic microbiota	115:176	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	7	45	with	 consistent	954:964	arg1	abundance					998:1006	the simultaneous increased abundance	971:1006	the simultaneous increased abundance of Methanobrevibacter smithii	971:1036	Methane was produced only at Dlow and pH > 7, consistent with the simultaneous increased abundance of Methanobrevibacter smithii.					
33864456	8	46	theme	microbiota	1109:1118	arg1	development					1087:1097	the development	1083:1097	the development of faecal microbiota in pectin-supplemented medium	1083:1148	Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium.					
33864456	1	47	theme	key	119:121	arg1	rate					106:109	gut transit rate	94:109	gut transit rate	94:109	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	47	theme	key	119:121	arg1	pH					87:88	Environmental pH	73:88	Environmental pH	73:88	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	1	47	theme	key	119:121	arg1	factors					123:129	the key factors	115:129	the key factors determining the dynamics of colonic microbiota	115:176	Environmental pH and gut transit rate are the key factors determining the dynamics of colonic microbiota.					
33864456	5	48	theme	gas	761:763	arg1	formation					765:773	Increased gas formation	751:773	Increased gas formation	751:773	Increased gas formation was observed at pH < 6.5.					
33864456	7	49	theme	simultaneous	975:986	arg1	abundance					998:1006	the simultaneous increased abundance	971:1006	the simultaneous increased abundance of Methanobrevibacter smithii	971:1036	Methane was produced only at Dlow and pH > 7, consistent with the simultaneous increased abundance of Methanobrevibacter smithii.					
33864456	2	50	theme	Dlow = 0.05	356:366	arg1	1/h					368:370	Dlow = 0.05 1/h	356:370	Dlow = 0.05 1/h	356:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	2	50	theme	Dlow = 0.05	356:366	arg1	rates					334:338	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h	300:370	In this study, the effect of changing pH on the composition and metabolism of pooled faecal microbiota was elucidated at physiologically relevant dilution rates Dhigh = 0.2 and Dlow = 0.05 1/h.					
33864456	4	51	theme	>7 at	665:669	arg1	Dhigh					671:675	pH >7 at Dhigh	662:675	pH >7 at Dhigh	662:675	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	4	52	theme	muciniphila	623:633	arg1	abundance					598:606	 while the abundance	587:606	 while the abundance of Akkermansia muciniphila	587:633	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
33864456	0	53	theme	faecal	54:59	arg1	microbiota					61:70	faecal microbiota	54:70	faecal microbiota	54:70	Acidic pH enhances butyrate production from pectin by faecal microbiota.					
33864456	9	54	theme	dietary	1173:1179	arg1	fibres					1181:1186	other dietary fibres	1167:1186	other dietary fibres	1167:1186	Fermentation of other dietary fibres can be studied using the same approach.					
33864456	8	55	from	development	1087:1097	arg1	medium					1143:1148	pectin-supplemented medium	1123:1148	pectin-supplemented medium	1123:1148	Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium.					
33864456	8	56	from	importance	1063:1072	arg1	development					1087:1097	the development	1083:1097	the development of faecal microbiota in pectin-supplemented medium	1083:1148	Our study confirmed the importance of pH in the development of faecal microbiota in pectin-supplemented medium.					
33864456	4	57	theme	Akkermansia	611:621	arg1	muciniphila					623:633	Akkermansia muciniphila	611:633	Akkermansia muciniphila	611:633	The butyrate producing Faecalibacterium and Coprococcus comes were extremely sensitive to pH > 7.5, while the abundance of Akkermansia muciniphila increased significantly at pH >7 at Dhigh, causing a pH-dependant shift in the dynamics of mucin degrading species.					
34619272	0	0	theme	acid	107:110	arg1	copolymerization					79:94	gamma-radiation copolymerization	63:94	gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	63:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	2	1	theme	nanoparticle	427:438	arg1	contents					440:447	MMT nanoparticle contents	423:447	MMT nanoparticle contents	423:447	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	4	2	theme	nanocomposite	819:831	arg1	films					843:847	homogeneous nanocomposite hydrogels films	807:847	homogeneous nanocomposite hydrogels films	807:847	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	2	3	theme	PAAc-co-	504:511	arg1	hydrogels					526:534	PAAc-co-(PLST/MMT/CS) hydrogels	504:534	PAAc-co-(PLST/MMT/CS) hydrogels	504:534	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	1	4	theme	nanocomposite	212:224	arg1	hydrogels					226:234	nanocomposite hydrogels	212:234	nanocomposite hydrogels	212:234	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	0	5	theme	acrylic	99:105	arg1	AAc					113:115	AAc	113:115	AAc	113:115	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	0	5	theme	acrylic	99:105	arg1	acid					107:110	acrylic acid	99:110	acrylic acid (AAc)	99:116	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	6	theme	plasticized	313:323	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	0	7	theme	MMT	171:173	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	2	8	theme	hydrogels	526:534	arg1	fraction					460:467	gel fraction	456:467	gel fraction	456:467	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	2	8	theme	hydrogels	526:534	arg1	absorption					479:488	water absorption	473:488	water absorption	473:488	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	1	9	theme	starch	325:330	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	6	10	theme	rat	1127:1129	arg1	models					1131:1136	rat models	1127:1136	rat models	1127:1136	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	0	11	theme	/chitosan	175:183	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	4	12	theme	highest	857:863	arg1	absorption					865:874	the highest absorption	853:874	the highest absorption in water	853:883	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	4	12	theme	highest	857:863	arg1	15 kGy					889:894	15 kGy	889:894	15 kGy	889:894	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	2	13	theme	gel	456:458	arg1	fraction					460:467	gel fraction	456:467	gel fraction	456:467	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	1	14	theme	PLST	333:336	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	4	15	theme	irradiation	784:794	arg1	absorption					865:874	the highest absorption	853:874	the highest absorption in water	853:883	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	4	15	theme	irradiation	784:794	arg1	dose					770:773	the appropriate dose	754:773	the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films	754:847	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	4	15	theme	irradiation	784:794	arg1	15 kGy					889:894	15 kGy	889:894	15 kGy	889:894	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	5	16	dep	5-wt	954:957	arg1	up					948:949	up	948:949	up	948:949	The introduction of MMT up to 5-wt (%) improved the physical properties and enhanced the drug uptake-release characters.					
34619272	5	17	theme	uptake-release	1018:1031	arg1	characters					1033:1042	the drug uptake-release characters	1009:1042	the drug uptake-release characters	1009:1042	The introduction of MMT up to 5-wt (%) improved the physical properties and enhanced the drug uptake-release characters.					
34619272	2	18	theme	MMT	423:425	arg1	contents					440:447	MMT nanoparticle contents	423:447	MMT nanoparticle contents	423:447	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	0	19	theme	CS	186:187	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	7	20	theme	potential	1350:1358	arg1	candidate					1360:1368	a potential candidate	1348:1368	a potential candidate for wound dressing materials	1348:1397	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	7	20	theme	potential	1350:1358	arg1	which					1321:1325	which	1321:1325	which	1321:1325	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	7	21	theme	dressing	1380:1387	arg1	materials					1389:1397	wound dressing materials	1374:1397	wound dressing materials	1374:1397	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	7	22	dep	healed	1307:1312	arg1	faster					1314:1319	faster	1314:1319	faster	1314:1319	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	2	23	theme	dose	414:417	arg1	effect					392:397	The effect	388:397	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels	388:534	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	1	24	theme	/montmorillonite	338:353	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	3	25	theme	X-ray	707:711	arg1	XRD					726:728	XRD	726:728	XRD	726:728	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	3	25	theme	X-ray	707:711	arg1	diffraction					713:723	X-ray diffraction	707:723	X-ray diffraction (XRD)	707:729	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	4	26	from	dose	770:773	arg1	water					879:883	water	879:883	water	879:883	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	3	27	theme	FTIR	651:654	arg1	spectroscopy					656:667	FTIR spectroscopy	651:667	FTIR spectroscopy	651:667	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	0	28	theme	Biological	0:9	arg1	applications					11:22	Biological applications	0:22	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.	0:196	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	2	29	dep	PAAc-co-	504:511	arg1	PLST/MMT/CS					513:523	PLST/MMT/CS	513:523	PLST/MMT/CS	513:523	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	4	30	from	absorption	865:874	arg1	water					879:883	water	879:883	water	879:883	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	3	31	theme	nanocomposite	606:618	arg1	hydrogels					620:628	the nanocomposite hydrogels	602:628	the nanocomposite hydrogels	602:628	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	0	32	theme	nanocomposite	27:39	arg1	hydrogels					41:49	nanocomposite hydrogels	27:49	nanocomposite hydrogels	27:49	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	33	theme	gamma-radiation	253:267	arg1	copolymerization					269:284	gamma-radiation copolymerization	253:284	gamma-radiation copolymerization of acrylic acid (AAc)	253:306	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	0	34	theme	starch	135:140	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	4	35	theme	homogeneous	807:817	arg1	films					843:847	homogeneous nanocomposite hydrogels films	807:847	homogeneous nanocomposite hydrogels films	807:847	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	6	36	theme	wound	1095:1099	arg1	healing					1101:1107	skin wound healing	1090:1107	skin wound healing	1090:1107	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	0	37	theme	plasticized	123:133	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	38	theme	clay	355:358	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	4	39	dep	dose	770:773	arg1	achieve					799:805	achieve	799:805	to achieve homogeneous nanocomposite hydrogels films	796:847	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	6	40	theme	nanocomposite	1063:1075	arg1	hydrogels					1077:1085	the nanocomposite hydrogels	1059:1085	the nanocomposite hydrogels	1059:1085	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	0	41	theme	PLST	143:146	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	4	42	theme	appropriate	758:768	arg1	dose					770:773	the appropriate dose	754:773	the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films	754:847	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	4	42	theme	appropriate	758:768	arg1	15 kGy					889:894	15 kGy	889:894	15 kGy	889:894	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	7	43	theme	time	1218:1221	arg1	intervals					1223:1231	different time intervals	1208:1231	different time intervals up 21 days	1208:1242	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	0	44	theme	hydrogels	41:49	arg1	applications					11:22	Biological applications	0:22	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.	0:196	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	45	theme	acrylic	289:295	arg1	acid					297:300	acrylic acid	289:300	acrylic acid (AAc)	289:306	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	45	theme	acrylic	289:295	arg1	AAc					303:305	AAc	303:305	AAc	303:305	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	2	46	theme	water	473:477	arg1	absorption					479:488	water absorption	473:488	water absorption	473:488	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	1	47	theme	MMT	361:363	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	6	48	theme	model	1165:1169	arg1	sulfanilamide					1146:1158	sulfanilamide	1146:1158	sulfanilamide	1146:1158	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	6	48	theme	model	1165:1169	arg1	drug					1171:1174	a model drug	1163:1174	a model drug	1163:1174	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	0	49	theme	gamma-radiation	63:77	arg1	copolymerization					79:94	gamma-radiation copolymerization	63:94	gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	63:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	50	theme	acid	297:300	arg1	copolymerization					269:284	gamma-radiation copolymerization	253:284	gamma-radiation copolymerization of acrylic acid (AAc)	253:306	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	0	51	theme	/montmorillonite	148:163	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	2	52	theme	contents	440:447	arg1	effect					392:397	The effect	388:397	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels	388:534	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	3	53	theme	thermogravimetric	670:686	arg1	analysis					688:695	thermogravimetric analysis	670:695	thermogravimetric analysis (TGA)	670:701	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	3	53	theme	thermogravimetric	670:686	arg1	TGA					698:700	TGA	698:700	TGA	698:700	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	6	54	theme	hydrogels	1077:1085	arg1	effect					1049:1054	The effect	1045:1054	The effect of the nanocomposite hydrogels on skin wound healing	1045:1107	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	2	55	dep	fraction	460:467	arg1	characters					490:499	characters	490:499	characters	490:499	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	2	55	dep	fraction	460:467	arg1	the					452:454	the	452:454	the	452:454	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	1	56	theme	/chitosan	365:373	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	4	57	theme	gamma	778:782	arg1	irradiation					784:794	gamma irradiation	778:794	gamma irradiation	778:794	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	3	58	theme	structure-property	571:588	arg1	behavior					590:597	the structure-property behavior	567:597	the structure-property behavior of the nanocomposite hydrogels	567:628	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	5	59	theme	physical	976:983	arg1	properties					985:994	the physical properties	972:994	the physical properties	972:994	The introduction of MMT up to 5-wt (%) improved the physical properties and enhanced the drug uptake-release characters.					
34619272	5	60	theme	MMT	944:946	arg1	introduction					928:939	The introduction	924:939	The introduction of MMT up to 5-wt (%)	924:961	The introduction of MMT up to 5-wt (%) improved the physical properties and enhanced the drug uptake-release characters.					
34619272	5	61	theme	drug	1013:1016	arg1	characters					1033:1042	the drug uptake-release characters	1009:1042	the drug uptake-release characters	1009:1042	The introduction of MMT up to 5-wt (%) improved the physical properties and enhanced the drug uptake-release characters.					
34619272	7	62	theme	rat	1193:1195	arg1	skin					1197:1200	rat skin	1193:1200	rat skin	1193:1200	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	6	63	theme	skin	1090:1093	arg1	healing					1101:1107	skin wound healing	1090:1107	skin wound healing	1090:1107	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
34619272	0	64	theme	clay	165:168	arg1	blends					190:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	123:195	Biological applications of nanocomposite hydrogels prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	1	65	theme	CS	376:377	arg1	blends					380:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends	313:385	In this work, nanocomposite hydrogels were prepared by gamma-radiation copolymerization of acrylic acid (AAc) onto plasticized starch (PLST)/montmorillonite clay (MMT)/chitosan (CS) blends.					
34619272	7	66	theme	copolymer	1282:1290	arg1	hydrogels					1292:1300	the copolymer hydrogels	1278:1300	the copolymer hydrogels	1278:1300	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	7	67	theme	skin	1197:1200	arg1	profiles					1181:1188	The profiles	1177:1188	The profiles of rat skin after different time intervals up 21 days	1177:1242	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	4	68	theme	hydrogels	833:841	arg1	films					843:847	homogeneous nanocomposite hydrogels films	807:847	homogeneous nanocomposite hydrogels films	807:847	The study showed that the appropriate dose of gamma irradiation to achieve homogeneous nanocomposite hydrogels films and the highest absorption in water was 15 kGy, regardless of composition.					
34619272	2	69	from	effect	392:397	arg1	fraction					460:467	gel fraction	456:467	gel fraction	456:467	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	2	69	from	effect	392:397	arg1	absorption					479:488	water absorption	473:488	water absorption	473:488	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	3	70	theme	hydrogels	620:628	arg1	behavior					590:597	the structure-property behavior	567:597	the structure-property behavior of the nanocomposite hydrogels	567:628	In addition, the structure-property behavior of the nanocomposite hydrogels was characterized by FTIR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD).					
34619272	7	71	theme	wound	1374:1378	arg1	materials					1389:1397	wound dressing materials	1374:1397	wound dressing materials	1374:1397	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	2	72	theme	irradiation	402:412	arg1	dose					414:417	irradiation dose	402:417	irradiation dose	402:417	The effect of irradiation dose and MMT nanoparticle contents on the gel fraction and water absorption characters of PAAc-co-(PLST/MMT/CS) hydrogels was investigated.					
34619272	7	73	theme	different	1208:1216	arg1	intervals					1223:1231	different time intervals	1208:1231	different time intervals up 21 days	1208:1242	The profiles of rat skin after different time intervals up 21 days revealed that wounds treated with the copolymer hydrogels were healed faster which it may considered as a potential candidate for wound dressing materials.					
34619272	6	74	from	effect	1049:1054	arg1	healing					1101:1107	skin wound healing	1090:1107	skin wound healing	1090:1107	The effect of the nanocomposite hydrogels on skin wound healing were evaluated by rat models, taking sulfanilamide as a model drug.					
31962071	0	0	theme	chitosan/metal-organic	77:98	arg1	composite					110:118	chitosan/metal-organic framework composite	77:118	chitosan/metal-organic framework composite	77:118	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	3	1	theme	weak	785:788	arg1	acid					790:793	weak acid	785:793	weak acid	785:793	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	3	2	contain	have	677:680	arg1	pH					674:675	pH	674:675	pH	674:675	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	3	2	contain	have	677:680	arg1	temperature					658:668	temperature	658:668	temperature	658:668	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	3	2	contain	have	677:680	arg2	influence					690:698	a great influence	682:698	a great influence on adsorption	682:712	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	2	3	theme	gravimetric	548:558	arg1	analyzer					560:567	Thermal gravimetric analyzer	540:567	Thermal gravimetric analyzer	540:567	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	3	4	theme	low	765:767	arg1	temperature					769:779	low temperature	765:779	low temperature	765:779	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	2	5	theme	powder	378:383	arg1	diffraction					385:395	X-ray powder diffraction	372:395	X-ray powder diffraction	372:395	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	6	6	theme	many	1076:1079	arg1	times					1081:1085	many times	1076:1085	many times	1076:1085	ILCS/U-10 also has good stability and can be recycled many times.					
31962071	2	7	theme	Zeta	605:608	arg1	potential					610:618	Zeta potential	605:618	Zeta potential	605:618	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	0	8	from	Removal	0:6	arg1	solution					43:50	aqueous solution	35:50	aqueous solution	35:50	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	3	9	theme	Adsorption	621:630	arg1	experiments					632:642	Adsorption experiments	621:642	Adsorption experiments	621:642	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	0	10	theme	framework	100:108	arg1	composite					110:118	chitosan/metal-organic framework composite	77:118	chitosan/metal-organic framework composite	77:118	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	4	11	theme	order	840:844	arg1	model					846:850	pseudo-second order model	826:850	pseudo-second order model	826:850	The dependence of ILCS/U-X on pseudo-second order model and Temkin model was observed.					
31962071	3	12	from	influence	690:698	arg1	adsorption					703:712	adsorption	703:712	adsorption	703:712	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	1	13	theme	novel	125:129	arg1	materials					147:155	The novel ILCS/U-X powder materials	121:155	The novel ILCS/U-X powder materials	121:155	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	5	14	contain	has	935:937	arg2	capacity					962:969	the largest adsorption capacity	939:969	the largest adsorption capacity	939:969	Compared to other ratios, the Micro-doped ILCS/U-10 has the largest adsorption capacity and can quickly complete adsorption within 60 min.					
31962071	5	14	contain	has	935:937	arg1	ILCS/U-10					925:933	the Micro-doped ILCS/U-10	909:933	the Micro-doped ILCS/U-10	909:933	Compared to other ratios, the Micro-doped ILCS/U-10 has the largest adsorption capacity and can quickly complete adsorption within 60 min.					
31962071	2	15	dep	Fourier	398:404	arg1	transform					406:414	transform	406:414	transform infra-red	406:424	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	5	16	theme	largest	943:949	arg1	capacity					962:969	the largest adsorption capacity	939:969	the largest adsorption capacity	939:969	Compared to other ratios, the Micro-doped ILCS/U-10 has the largest adsorption capacity and can quickly complete adsorption within 60 min.					
31962071	2	17	theme	X-ray	427:431	arg1	spectroscopy					447:458	X-ray photoelectron spectroscopy	427:458	X-ray photoelectron spectroscopy	427:458	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	2	18	theme	dispersive	577:586	arg1	spectrometer					588:599	Energy dispersive spectrometer	570:599	Energy dispersive spectrometer	570:599	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	2	19	theme	Thermal	540:546	arg1	analyzer					560:567	Thermal gravimetric analyzer	540:567	Thermal gravimetric analyzer	540:567	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	6	20	theme	good	1041:1044	arg1	stability					1046:1054	good stability	1041:1054	good stability	1041:1054	ILCS/U-10 also has good stability and can be recycled many times.					
31962071	4	21	theme	ILCS/U-X	814:821	arg1	dependence					800:809	The dependence	796:809	The dependence of ILCS/U-X on pseudo-second order model and Temkin model	796:867	The dependence of ILCS/U-X on pseudo-second order model and Temkin model was observed.					
31962071	0	22	from	solution	43:50	arg1	Removal					0:6	Removal	0:6	Removal of organic herbicides from aqueous solution by ionic liquid	0:66	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	0	22	from	solution	43:50	arg1	herbicides					19:28	organic herbicides	11:28	organic herbicides from aqueous solution	11:50	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	1	23	theme	ionic	197:201	arg1	chitosan					219:226	ionic liquid modified chitosan	197:226	ionic liquid modified chitosan	197:226	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	1	24	theme	2,4-dichlorophenoxyacetic	286:310	arg1	acid					312:315	2,4-dichlorophenoxyacetic acid	286:315	2,4-dichlorophenoxyacetic acid in aqueous solution	286:335	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	1	25	from	acid	312:315	arg1	solution					328:335	aqueous solution	320:335	aqueous solution	320:335	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	1	26	theme	liquid	203:208	arg1	chitosan					219:226	ionic liquid modified chitosan	197:226	ionic liquid modified chitosan	197:226	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	0	27	theme	herbicides	19:28	arg1	Removal					0:6	Removal	0:6	Removal of organic herbicides from aqueous solution by ionic liquid	0:66	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	7	28	theme	important	1152:1160	arg1	bonding					1097:1103	Hydrogen bonding	1088:1103	Hydrogen bonding	1088:1103	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	28	theme	important	1152:1160	arg1	interaction					1123:1133	electrostatic interaction	1109:1133	electrostatic interaction	1109:1133	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	28	theme	important	1152:1160	arg1	forces					1162:1167	the two most important forces	1139:1167	the two most important forces that dominate adsorption	1139:1192	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	4	29	theme	pseudo-second	826:838	arg1	model					846:850	pseudo-second order model	826:850	pseudo-second order model	826:850	The dependence of ILCS/U-X on pseudo-second order model and Temkin model was observed.					
31962071	1	30	theme	modified	210:217	arg1	chitosan					219:226	ionic liquid modified chitosan	197:226	ionic liquid modified chitosan	197:226	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	0	31	theme	organic	11:17	arg1	herbicides					19:28	organic herbicides	11:28	organic herbicides from aqueous solution	11:50	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	4	32	theme	Temkin	856:861	arg1	model					863:867	Temkin model	856:867	Temkin model	856:867	The dependence of ILCS/U-X on pseudo-second order model and Temkin model was observed.					
31962071	5	33	theme	other	895:899	arg1	ratios					901:906	other ratios	895:906	other ratios	895:906	Compared to other ratios, the Micro-doped ILCS/U-10 has the largest adsorption capacity and can quickly complete adsorption within 60 min.					
31962071	0	34	theme	aqueous	35:41	arg1	solution					43:50	aqueous solution	35:50	aqueous solution	35:50	Removal of organic herbicides from aqueous solution by ionic liquid modified chitosan/metal-organic framework composite.					
31962071	1	35	theme	aqueous	320:326	arg1	solution					328:335	aqueous solution	320:335	aqueous solution	320:335	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	5	36	theme	Micro-doped	913:923	arg1	ILCS/U-10					925:933	the Micro-doped ILCS/U-10	909:933	the Micro-doped ILCS/U-10	909:933	Compared to other ratios, the Micro-doped ILCS/U-10 has the largest adsorption capacity and can quickly complete adsorption within 60 min.					
31962071	5	37	theme	adsorption	951:960	arg1	capacity					962:969	the largest adsorption capacity	939:969	the largest adsorption capacity	939:969	Compared to other ratios, the Micro-doped ILCS/U-10 has the largest adsorption capacity and can quickly complete adsorption within 60 min.					
31962071	3	38	contain	have	734:737	arg1	adsorbents					723:732	all adsorbents	719:732	all adsorbents	719:732	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	3	38	contain	have	734:737	arg2	adsorption					751:760	the highest adsorption	739:760	the highest adsorption	739:760	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	1	39	used	used	271:274	arg2	materials					256:264	different doping ratio materials	233:264	different doping ratio materials	233:264	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	3	40	theme	great	684:688	arg1	influence					690:698	a great influence	682:698	a great influence on adsorption	682:712	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	2	41	theme	photoelectron	433:445	arg1	spectroscopy					447:458	X-ray photoelectron spectroscopy	427:458	X-ray photoelectron spectroscopy	427:458	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	2	42	theme	X-ray	372:376	arg1	diffraction					385:395	X-ray powder diffraction	372:395	X-ray powder diffraction	372:395	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	1	43	theme	ILCS/U-X	131:138	arg1	materials					147:155	The novel ILCS/U-X powder materials	121:155	The novel ILCS/U-X powder materials	121:155	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	4	44	from	dependence	800:809	arg1	model					846:850	pseudo-second order model	826:850	pseudo-second order model	826:850	The dependence of ILCS/U-X on pseudo-second order model and Temkin model was observed.					
31962071	4	44	from	dependence	800:809	arg1	model					863:867	Temkin model	856:867	Temkin model	856:867	The dependence of ILCS/U-X on pseudo-second order model and Temkin model was observed.					
31962071	6	45	contain	has	1037:1039	arg2	stability					1046:1054	good stability	1041:1054	good stability	1041:1054	ILCS/U-10 also has good stability and can be recycled many times.					
31962071	6	45	contain	has	1037:1039	arg1	ILCS/U-10					1022:1030	ILCS/U-10	1022:1030	ILCS/U-10	1022:1030	ILCS/U-10 also has good stability and can be recycled many times.					
31962071	7	46	theme	electrostatic	1109:1121	arg1	bonding					1097:1103	Hydrogen bonding	1088:1103	Hydrogen bonding	1088:1103	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	46	theme	electrostatic	1109:1121	arg1	interaction					1123:1133	electrostatic interaction	1109:1133	electrostatic interaction	1109:1133	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	46	theme	electrostatic	1109:1121	arg1	forces					1162:1167	the two most important forces	1139:1167	the two most important forces that dominate adsorption	1139:1192	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	47	theme	Hydrogen	1088:1095	arg1	bonding					1097:1103	Hydrogen bonding	1088:1103	Hydrogen bonding	1088:1103	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	47	theme	Hydrogen	1088:1095	arg1	interaction					1123:1133	electrostatic interaction	1109:1133	electrostatic interaction	1109:1133	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	7	47	theme	Hydrogen	1088:1095	arg1	forces					1162:1167	the two most important forces	1139:1167	the two most important forces that dominate adsorption	1139:1192	Hydrogen bonding and electrostatic interaction are the two most important forces that dominate adsorption.					
31962071	1	48	theme	powder	140:145	arg1	materials					147:155	The novel ILCS/U-X powder materials	121:155	The novel ILCS/U-X powder materials	121:155	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	1	49	theme	different	233:241	arg1	materials					256:264	different doping ratio materials	233:264	different doping ratio materials	233:264	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	1	50	theme	doping	243:248	arg1	materials					256:264	different doping ratio materials	233:264	different doping ratio materials	233:264	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	2	51	theme	Energy	570:575	arg1	spectrometer					588:599	Energy dispersive spectrometer	570:599	Energy dispersive spectrometer	570:599	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	3	52	theme	highest	743:749	arg1	adsorption					751:760	the highest adsorption	739:760	the highest adsorption	739:760	Adsorption experiments revealed that temperature and pH have a great influence on adsorption, and all adsorbents have the highest adsorption at low temperature and weak acid.					
31962071	2	53	theme	Scanning	461:468	arg1	microscopy					479:488	Scanning electron microscopy	461:488	Scanning electron microscopy	461:488	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	1	54	theme	ratio	250:254	arg1	materials					256:264	different doping ratio materials	233:264	different doping ratio materials	233:264	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
31962071	2	55	theme	electron	470:477	arg1	microscopy					479:488	Scanning electron microscopy	461:488	Scanning electron microscopy	461:488	The samples were characterized by X-ray powder diffraction, Fourier transform infra-red, X-ray photoelectron spectroscopy, Scanning electron microscopy, Brunauer-Emmett-Teller, Barrette-Joyner-Halenda, Thermal gravimetric analyzer, Energy dispersive spectrometer and Zeta potential.					
31962071	1	56	from	UiO-66	187:192	arg1	chitosan					219:226	ionic liquid modified chitosan	197:226	ionic liquid modified chitosan	197:226	The novel ILCS/U-X powder materials were fabricated by depositing UiO-66 on ionic liquid modified chitosan, and different doping ratio materials were used to adsorb 2,4-dichlorophenoxyacetic acid in aqueous solution.					
33065965	12	0	theme	lignin	1646:1651	arg1	content					1653:1659	the partial lignin content	1634:1659	the partial lignin content in MF from kraft paper	1634:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	10	1	theme	properties	1383:1392	arg1	modification					1349:1360	the modification	1345:1360	the modification of the water barrier properties according to the required features	1345:1427	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	2	2	theme	production	351:360	arg1	costs					362:366	the current production costs	339:366	the current production costs	339:366	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	7	3	theme	neat	1002:1005	arg1	samples					1011:1017	neat PHA samples	1002:1017	neat PHA samples	1002:1017	Moreover, PHA films reinforced with MF are 23% stronger than neat PHA samples.					
33065965	3	4	theme	acid	543:546	arg1	films					554:558	polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films	501:558	polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films	501:558	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	10	5	theme	required	1411:1418	arg1	features					1420:1427	the required features	1407:1427	the required features	1407:1427	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	13	6	theme	kraft	1761:1765	arg1	paper					1767:1771	kraft paper	1761:1771	kraft paper	1761:1771	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
33065965	2	7	theme	composite	276:284	arg1	products					286:293	composite products	276:293	composite products	276:293	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	5	8	theme	better	811:816	arg1	effect					830:835	a better reinforcing effect	809:835	a better reinforcing effect on the tensile strength of PLA samples	809:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	4	9	theme	biopolymers	638:648	arg1	properties					624:633	the properties	620:633	the properties of biopolymers	620:648	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	1	10	theme	mechanical	185:194	arg1	properties					209:218	excellent mechanical and physical properties	175:218	excellent mechanical and physical properties	175:218	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	4	11	theme	filler	601:606	arg1	material					608:615	filler material	601:615	filler material	601:615	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	2	12	theme	material	305:312	arg1	demand					314:319	high material demand	300:319	high material demand	300:319	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	1	13	theme	physical	200:207	arg1	properties					209:218	excellent mechanical and physical properties	175:218	excellent mechanical and physical properties	175:218	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	9	14	from	increase	1152:1159	arg1	crystallinity					1168:1180	the crystallinity	1164:1180	the crystallinity of PHA	1164:1187	Additionally, the addition of MF leads to an increase in the crystallinity of PHA that can be controlled by heat treatment followed by quenching.					
33065965	2	15	with	application	261:271	arg1	demand					314:319	high material demand	300:319	high material demand	300:319	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	8	16	theme	material	1097:1104	arg1	stiffness					1080:1088	the stiffness	1076:1088	the stiffness of the material	1076:1104	This gain in strength is accompanied by an increment of the stiffness of the material.					
33065965	11	17	from	bonding	1580:1586	arg1	applications					1613:1624	multi-layer composite applications	1591:1624	multi-layer composite applications	1591:1624	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	7	18	theme	PHA	951:953	arg1	films					955:959	PHA films	951:959	PHA films reinforced with MF	951:978	Moreover, PHA films reinforced with MF are 23% stronger than neat PHA samples.					
33065965	5	19	theme	PLA	864:866	arg1	samples					868:874	PLA samples	864:874	PLA samples	864:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	12	20	from	paper	1678:1682	arg1	MF					1664:1665	MF	1664:1665	MF from kraft paper	1664:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	12	20	from	paper	1678:1682	arg1	content					1653:1659	the partial lignin content	1634:1659	the partial lignin content in MF from kraft paper	1634:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	5	21	dep	wt	893:894	arg1	20					890:891	20	890:891	20	890:891	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	11	22	theme	multi-layer	1591:1601	arg1	applications					1613:1624	multi-layer composite applications	1591:1624	multi-layer composite applications	1591:1624	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	9	23	theme	MF	1137:1138	arg1	addition					1125:1132	the addition	1121:1132	the addition of MF	1121:1138	Additionally, the addition of MF leads to an increase in the crystallinity of PHA that can be controlled by heat treatment followed by quenching.					
33065965	3	24	theme	polylactic	532:541	arg1	PLA					549:551	PLA	549:551	PLA	549:551	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	3	24	theme	polylactic	532:541	arg1	acid					543:546	polylactic acid	532:546	polylactic acid (PLA)	532:552	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	13	25	from	paper	1767:1771	arg1	MF					1753:1754	MF	1753:1754	MF from kraft paper	1753:1771	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
33065965	13	25	from	paper	1767:1771	arg1	way					1794:1796	a way to introduce high fiber concentrations (up to 20 wt	1792:1848	a way to introduce high fiber concentrations (up to 20 wt	1792:1848	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
33065965	6	26	theme	PLA	930:932	arg1	films					934:938	stronger PLA films	921:938	stronger PLA films	921:938	% of MF may also lead to stronger PLA films.					
33065965	0	27	theme	Biopolymers	51:61	arg1	Reinforcement					63:75	PLA Biopolymers Reinforcement	47:75	PLA Biopolymers Reinforcement	47:75	Potential of Cellulose Microfibers for PHA and PLA Biopolymers Reinforcement.					
33065965	5	28	theme	mechanical	778:787	arg1	properties					789:798	mechanical properties	778:798	mechanical properties	778:798	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	1	29	theme	many	139:142	arg1	fields					156:161	many engineering fields	139:161	many engineering fields	139:161	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	14	30	theme	economic	1927:1934	arg1	benefits					1950:1957	economic and technical benefits	1927:1957	economic and technical benefits	1927:1957	%) from other sources of recycled paper into biocomposite manufacturing with economic and technical benefits.					
33065965	5	31	dep	though	877:882	arg1	wt					893:894	wt	893:894	wt	893:894	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	4	32	from	influence	575:583	arg1	properties					624:633	the properties	620:633	the properties of biopolymers	620:648	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	1	33	theme	fields	156:161	arg1	attention					126:134	the attention	122:134	the attention of many engineering fields	122:161	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	10	34	theme	PHA	1289:1291	arg1	crystallinity					1272:1284	the crystallinity	1268:1284	the crystallinity of PHA	1268:1291	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	4	35	theme	different	685:693	arg1	scales					695:700	different scales	685:700	different scales	685:700	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	6	36	theme	MF	901:902	arg1	MF					901:902	MF	901:902	MF	901:902	% of MF may also lead to stronger PLA films.					
33065965	6	36	theme	MF	901:902	arg1	%					896:896	%	896:896	% of MF	896:902	% of MF may also lead to stronger PLA films.					
33065965	10	37	theme	samples	1327:1333	arg1	hygroscopicity					1305:1318	the hygroscopicity	1301:1318	the hygroscopicity of PHA samples	1301:1333	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	12	38	theme	UV	1722:1723	arg1	effect					1734:1739	a UV blocking effect	1720:1739	a UV blocking effect	1720:1739	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	1	39	theme	Cellulose	78:86	arg1	CNC					102:104	CNC	102:104	CNC	102:104	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	1	39	theme	Cellulose	78:86	arg1	nanocrystals					88:99	Cellulose nanocrystals	78:99	Cellulose nanocrystals (CNC)	78:105	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	13	40	dep	concentrations	1822:1835	arg1	wt					1847:1848	up to 20 wt	1838:1848	up to 20 wt	1838:1848	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
33065965	14	41	theme	recycled	1875:1882	arg1	paper					1884:1888	recycled paper	1875:1888	recycled paper into biocomposite	1875:1906	%) from other sources of recycled paper into biocomposite manufacturing with economic and technical benefits.					
33065965	3	42	theme	water	456:460	arg1	dispersion					462:471	a straightforward water dispersion	438:471	a straightforward water dispersion of kraft paper	438:486	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	0	43	theme	Cellulose	13:21	arg1	Microfibers					23:33	Cellulose Microfibers	13:33	Cellulose Microfibers	13:33	Potential of Cellulose Microfibers for PHA and PLA Biopolymers Reinforcement.					
33065965	3	44	theme	microfibers	409:419	arg1	use					392:394	the use	388:394	the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films	388:558	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	14	45	from	sources	1864:1870	arg1	%					1850:1850	%)	1850:1851	%) from other sources of recycled paper into biocomposite	1850:1906	%) from other sources of recycled paper into biocomposite manufacturing with economic and technical benefits.					
33065965	10	46	theme	barrier	1375:1381	arg1	properties					1383:1392	the water barrier properties	1365:1392	the water barrier properties according to the required features	1365:1427	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	2	47	theme	current	343:349	arg1	costs					362:366	the current production costs	339:366	the current production costs	339:366	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	3	48	theme	paper	482:486	arg1	dispersion					462:471	a straightforward water dispersion	438:471	a straightforward water dispersion of kraft paper	438:486	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	12	49	theme	kraft	1672:1676	arg1	paper					1678:1682	kraft paper	1672:1682	kraft paper	1672:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	11	50	theme	interlaminar	1567:1578	arg1	bonding					1580:1586	better interlaminar bonding	1560:1586	better interlaminar bonding in multi-layer composite applications	1560:1624	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	1	51	theme	polymer	223:229	arg1	reinforcement					231:243	polymer reinforcement	223:243	polymer reinforcement	223:243	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	12	52	theme	partial	1638:1644	arg1	content					1653:1659	the partial lignin content	1634:1659	the partial lignin content in MF from kraft paper	1634:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	11	53	theme	polymers	1466:1473	arg1	types					1457:1461	both types	1452:1461	both types of polymers	1452:1473	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	10	54	from	change	1258:1263	arg1	crystallinity					1272:1284	the crystallinity	1268:1284	the crystallinity of PHA	1268:1291	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	11	55	theme	surface	1494:1500	arg1	roughness					1502:1510	the surface roughness	1490:1510	the surface roughness	1490:1510	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	7	56	theme	PHA	1007:1009	arg1	samples					1011:1017	neat PHA samples	1002:1017	neat PHA samples	1002:1017	Moreover, PHA films reinforced with MF are 23% stronger than neat PHA samples.					
33065965	13	57	theme	fiber	1816:1820	arg1	concentrations					1822:1835	high fiber concentrations	1811:1835	high fiber concentrations (up to 20 wt	1811:1848	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
33065965	2	58	theme	high	300:303	arg1	demand					314:319	high material demand	300:319	high material demand	300:319	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	5	59	theme	tensile	844:850	arg1	strength					852:859	the tensile strength	840:859	the tensile strength of PLA samples	840:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	11	60	theme	films	1519:1523	arg1	roughness					1502:1510	the surface roughness	1490:1510	the surface roughness	1490:1510	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	5	61	theme	reinforcing	818:828	arg1	effect					830:835	a better reinforcing effect	809:835	a better reinforcing effect on the tensile strength of PLA samples	809:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	1	62	theme	excellent	175:183	arg1	properties					209:218	excellent mechanical and physical properties	175:218	excellent mechanical and physical properties	175:218	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	8	63	theme	stiffness	1080:1088	arg1	increment					1063:1071	an increment	1060:1071	an increment of the stiffness of the material	1060:1104	This gain in strength is accompanied by an increment of the stiffness of the material.					
33065965	4	64	theme	material	608:615	arg1	type					593:596	this type	588:596	this type of filler material	588:615	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	14	65	theme	technical	1940:1948	arg1	benefits					1950:1957	economic and technical benefits	1927:1957	economic and technical benefits	1927:1957	%) from other sources of recycled paper into biocomposite manufacturing with economic and technical benefits.					
33065965	5	66	theme	samples	868:874	arg1	strength					852:859	the tensile strength	840:859	the tensile strength of PLA samples	840:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	11	67	theme	composite	1603:1611	arg1	applications					1613:1624	multi-layer composite applications	1591:1624	multi-layer composite applications	1591:1624	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	5	68	from	effect	830:835	arg1	strength					852:859	the tensile strength	840:859	the tensile strength of PLA samples	840:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	6	69	theme	stronger	921:928	arg1	films					934:938	stronger PLA films	921:938	stronger PLA films	921:938	% of MF may also lead to stronger PLA films.					
33065965	5	70	dep	20	890:891	arg1	to					887:888	to	887:888	to	887:888	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	1	71	theme	engineering	144:154	arg1	fields					156:161	many engineering fields	139:161	many engineering fields	139:161	Cellulose nanocrystals (CNC) have attracted the attention of many engineering fields and offered excellent mechanical and physical properties as polymer reinforcement.					
33065965	12	72	theme	blocking	1725:1732	arg1	effect					1734:1739	a UV blocking effect	1720:1739	a UV blocking effect	1720:1739	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	10	73	theme	PHA	1323:1325	arg1	samples					1327:1333	PHA samples	1323:1333	PHA samples	1323:1333	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	5	74	contain	has	805:807	arg1	CNC					801:803	CNC	801:803	CNC	801:803	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	5	74	contain	has	805:807	arg2	effect					830:835	a better reinforcing effect	809:835	a better reinforcing effect on the tensile strength of PLA samples	809:874	Regarding mechanical properties, CNC has a better reinforcing effect on the tensile strength of PLA samples, though up to 20 wt.					
33065965	9	75	theme	PHA	1185:1187	arg1	crystallinity					1168:1180	the crystallinity	1164:1180	the crystallinity of PHA	1164:1187	Additionally, the addition of MF leads to an increase in the crystallinity of PHA that can be controlled by heat treatment followed by quenching.					
33065965	0	76	theme	Microfibers	23:33	arg1	Potential					0:8	Potential	0:8	Potential of Cellulose Microfibers for PHA and PLA Biopolymers Reinforcement.	0:76	Potential of Cellulose Microfibers for PHA and PLA Biopolymers Reinforcement.					
33065965	4	77	from	scales	695:700	arg1	cast					662:665	cast	662:665	cast	662:665	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	14	78	theme	other	1858:1862	arg1	sources					1864:1870	other sources	1858:1870	other sources of recycled paper into biocomposite	1858:1906	%) from other sources of recycled paper into biocomposite manufacturing with economic and technical benefits.					
33065965	8	79	from	gain	1025:1028	arg1	strength					1033:1040	strength	1033:1040	strength	1033:1040	This gain in strength is accompanied by an increment of the stiffness of the material.					
33065965	2	80	from	application	261:271	arg1	products					286:293	composite products	276:293	composite products	276:293	However, their application in composite products with high material demand is complex due to the current production costs.					
33065965	11	81	theme	MF	1446:1447	arg1	addition					1434:1441	The addition	1430:1441	The addition of MF to both types of polymers	1430:1473	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	3	82	theme	straightforward	440:454	arg1	dispersion					462:471	a straightforward water dispersion	438:471	a straightforward water dispersion of kraft paper	438:486	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	0	83	theme	PLA	47:49	arg1	Reinforcement					63:75	PLA Biopolymers Reinforcement	47:75	PLA Biopolymers Reinforcement	47:75	Potential of Cellulose Microfibers for PHA and PLA Biopolymers Reinforcement.					
33065965	9	84	theme	heat	1215:1218	arg1	treatment					1220:1228	heat treatment	1215:1228	heat treatment followed by quenching	1215:1250	Additionally, the addition of MF leads to an increase in the crystallinity of PHA that can be controlled by heat treatment followed by quenching.					
33065965	3	85	theme	cellulose	399:407	arg1	MF					422:423	MF	422:423	MF	422:423	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	3	85	theme	cellulose	399:407	arg1	microfibers					409:419	cellulose microfibers	399:419	cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper	399:486	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	13	86	dep	20	1844:1845	arg1	to					1841:1842	to	1841:1842	to	1841:1842	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
33065965	10	87	theme	water	1369:1373	arg1	properties					1383:1392	the water barrier properties	1365:1392	the water barrier properties according to the required features	1365:1427	This change in the crystallinity of PHA affects the hygroscopicity of PHA samples, allowing the modification of the water barrier properties according to the required features.					
33065965	11	88	theme	better	1560:1565	arg1	bonding					1580:1586	better interlaminar bonding	1560:1586	better interlaminar bonding in multi-layer composite applications	1560:1624	The addition of MF to both types of polymers also increases the surface roughness of the films, which may contribute to obtaining better interlaminar bonding in multi-layer composite applications.					
33065965	14	89	theme	paper	1884:1888	arg1	sources					1864:1870	other sources	1858:1870	other sources of recycled paper into biocomposite	1858:1906	%) from other sources of recycled paper into biocomposite manufacturing with economic and technical benefits.					
33065965	3	90	theme	polyhydroxyalkanoate	501:520	arg1	films					554:558	polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films	501:558	polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films	501:558	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	3	91	theme	kraft	476:480	arg1	paper					482:486	kraft paper	476:486	kraft paper	476:486	This work explores the use of cellulose microfibers (MF) obtained by a straightforward water dispersion of kraft paper to reinforce polyhydroxyalkanoate (PHA) and polylactic acid (PLA) films.					
33065965	12	92	from	content	1653:1659	arg1	MF					1664:1665	MF	1664:1665	MF from kraft paper	1664:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	12	92	from	content	1653:1659	arg1	paper					1678:1682	kraft paper	1672:1682	kraft paper	1672:1682	Due to the partial lignin content in MF from kraft paper, samples reinforced with MF present a UV blocking effect.					
33065965	4	93	theme	type	593:596	arg1	influence					575:583	the influence	571:583	the influence of this type of filler material on the properties of biopolymers	571:648	To assess the influence of this type of filler material on the properties of biopolymers, films were cast and reinforced at different scales, with both CNC and MF separately, to compare their effectiveness.					
33065965	13	94	theme	high	1811:1814	arg1	concentrations					1822:1835	high fiber concentrations	1811:1835	high fiber concentrations (up to 20 wt	1811:1848	Therefore, MF from kraft paper may be explored as a way to introduce high fiber concentrations (up to 20 wt.					
34510198	12	0	theme	maternal	2388:2395	arg1	health					2408:2413	maternal and infant health	2388:2413	maternal and infant health	2388:2413	Further research is needed to investigate the role these relations play in maternal and infant health.					
34510198	0	1	theme	Glucose	154:160	arg1	Tolerance					162:170	Gestational Impaired Glucose Tolerance	133:170	Gestational Impaired Glucose Tolerance	133:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	8	2	theme	Shannon	1683:1689	arg1	diversity					1672:1680	microbiota diversity	1661:1680	microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors	1661:1737	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	8	2	theme	Shannon	1683:1689	arg1	index					1691:1695	Shannon index) [-1.7 (0.78); P = 0.029	1683:1720	Shannon index) [-1.7 (0.78); P = 0.029	1683:1720	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	1	3	theme	oligosaccharides	226:241	arg1	milk					190:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	3	theme	oligosaccharides	226:241	arg1	source					205:210	a rich source	198:210	a rich source of human milk oligosaccharides (HMOs) and bacteria	198:261	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	9	4	from	B-diversity	1802:1812	arg1	secretors					1924:1932	secretors	1924:1932	secretors	1924:1932	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	9	5	theme	ortholog	1864:1871	arg1	B-diversity					1873:1883	KEGG ortholog B-diversity	1859:1883	KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043)	1859:1919	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	12	6	theme	infant	2401:2406	arg1	health					2408:2413	maternal and infant health	2388:2413	maternal and infant health	2388:2413	Further research is needed to investigate the role these relations play in maternal and infant health.					
34510198	3	7	theme	community	521:529	arg1	composition					531:541	microbial community composition	511:541	microbial community composition	511:541	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	11	8	theme	microbial	2223:2231	arg1	composition					2233:2243	microbial composition	2223:2243	microbial composition	2223:2243	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	12	9	dep	role	2359:2362	arg1	play					2380:2383	play	2380:2383	play in maternal and infant health	2380:2413	Further research is needed to investigate the role these relations play in maternal and infant health.					
34510198	6	10	theme	regression	1137:1146	arg1	models					1148:1153	Poisson regression models	1129:1153	Poisson regression models (differential abundance)	1129:1178	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	10	theme	regression	1137:1146	arg1	abundance					1169:1177	differential abundance	1156:1177	differential abundance	1156:1177	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	9	11	dep	B-diversity	1802:1812	arg1	P = 0.036					1844:1852	P = 0.036	1844:1852	P = 0.036	1844:1852	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	9	11	dep	B-diversity	1802:1812	arg1	R2 = 0.040					1832:1841	weighted UniFrac R2 = 0.040	1815:1841	weighted UniFrac R2 = 0.040	1815:1841	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	1	12	theme	BACKGROUND	173:182	arg1	milk					190:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	12	theme	BACKGROUND	173:182	arg1	source					205:210	a rich source	198:210	a rich source of human milk oligosaccharides (HMOs) and bacteria	198:261	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	0	13	theme	Women	101:105	arg1	Cohort					91:96	a Cohort	89:96	a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance	89:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	5	14	theme	Milk	807:810	arg1	composition					823:833	Milk microbiota composition	807:833	Milk microbiota composition	807:833	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	6	15	theme	predicted	1208:1216	arg1	models					1200:1205	general linear models	1185:1205	general linear models (predicted microbial function)	1185:1236	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	15	theme	predicted	1208:1216	arg1	function					1228:1235	predicted microbial function	1208:1235	predicted microbial function	1208:1235	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	10	16	theme	P-false	2151:2157	arg1	pathways					2141:2148	metabolism- and infection-related pathways	2107:2148	metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2107:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	16	theme	P-false	2151:2157	arg1	rate < 0.1					2169:2178	P-false discovery rate < 0.1	2151:2178	P-false discovery rate < 0.1	2151:2178	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	7	17	theme	intolerance	1338:1348	arg1	race					1265:1268	race	1265:1268	race	1265:1268	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	17	theme	intolerance	1338:1348	arg1	frequency					1275:1283	frequency	1275:1283	frequency	1275:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	17	theme	intolerance	1338:1348	arg1	BMI					1260:1262	Prepregnancy BMI	1247:1262	Prepregnancy BMI	1247:1262	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	17	theme	intolerance	1338:1348	arg1	RESULTS					1239:1245	RESULTS Prepregnancy BMI, race, and frequency	1239:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance,	1239:1349	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	4	18	theme	completed	694:702	arg1	NCT01405547					730:740	NCT01405547	730:740	NCT01405547	730:740	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	4	18	theme	completed	694:702	arg1	study					723:727	a previously completed prospective cohort study (NCT01405547)	681:741	a previously completed prospective cohort study (NCT01405547)	681:741	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	9	19	dep	B-diversity	1873:1883	arg1	P = 0.043					1910:1918	P = 0.043	1910:1918	P = 0.043	1910:1918	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	9	19	dep	B-diversity	1873:1883	arg1	R2 = 0.039					1898:1907	R2 = 0.039	1898:1907	R2 = 0.039	1898:1907	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	7	20	theme	gestational	1318:1328	arg1	intolerance					1338:1348	gestational glucose intolerance	1318:1348	gestational glucose intolerance	1318:1348	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	6	21	theme	general	1185:1191	arg1	models					1200:1205	general linear models	1185:1205	general linear models (predicted microbial function)	1185:1236	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	21	theme	general	1185:1191	arg1	function					1228:1235	predicted microbial function	1208:1235	predicted microbial function	1208:1235	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	4	22	theme	cohort	716:721	arg1	NCT01405547					730:740	NCT01405547	730:740	NCT01405547	730:740	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	4	22	theme	cohort	716:721	arg1	study					723:727	a previously completed prospective cohort study (NCT01405547)	681:741	a previously completed prospective cohort study (NCT01405547)	681:741	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	0	23	theme	Gestational	133:143	arg1	Tolerance					162:170	Gestational Impaired Glucose Tolerance	133:170	Gestational Impaired Glucose Tolerance	133:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	8	24	dep	secretors	1537:1545	arg1	[B-estimate					1547:1557	[B-estimate	1547:1557	secretors [B-estimate (SE)	1537:1562	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	8	24	dep	secretors	1537:1545	arg1	SE					1560:1561	SE	1560:1561	SE	1560:1561	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	5	25	theme	rapid	900:904	arg1	HPLC					922:925	rapid high-throughput HPLC	900:925	rapid high-throughput HPLC	900:925	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	11	26	theme	predicted	2249:2257	arg1	functions					2269:2277	predicted microbial functions	2249:2277	predicted microbial functions	2249:2277	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	10	27	theme	genes	2088:2092	arg1	enrichment					2054:2063	enrichment	2054:2063	enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2054:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	9	28	from	B-diversity	1873:1883	arg1	secretors					1924:1932	secretors	1924:1932	secretors	1924:1932	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	3	29	from	function	557:564	arg1	women					579:583	women	579:583	women with high rates of gestational glucose intolerance	579:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	29	from	function	557:564	arg1	milk					569:572	milk	569:572	milk from women with high rates of gestational glucose intolerance	569:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	30	theme	high	590:593	arg1	rates					595:599	high rates	590:599	high rates of gestational glucose intolerance	590:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	5	31	theme	RNA	868:870	arg1	sequencing					877:886	V4-16S ribosomal RNA gene sequencing	851:886	V4-16S ribosomal RNA gene sequencing	851:886	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	0	32	from	Microbiota	21:30	arg1	Milk					41:44	Human Milk	35:44	Human Milk	35:44	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	3	33	dep	OBJECTIVES	344:353	arg1	were					370:373	were	370:373	were	370:373	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	0	34	theme	Months	68:73	arg1	Postpartum					75:84	3 Months Postpartum	66:84	3 Months Postpartum	66:84	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	6	35	theme	secretor	974:981	arg1	phenotype					990:998	maternal secretor status phenotype	965:998	maternal secretor status phenotype	965:998	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	11	36	theme	human	2282:2286	arg1	milk					2288:2291	human milk	2282:2291	human milk	2282:2291	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	0	37	from	Oligosaccharides	0:15	arg1	Milk					41:44	Human Milk	35:44	Human Milk	35:44	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	4	38	theme	milk	749:752	arg1	n = 107					763:769	n = 107	763:769	n = 107	763:769	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	4	38	theme	milk	749:752	arg1	samples					754:760	milk samples	749:760	milk samples (n = 107)	749:770	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	3	39	with	women	579:583	arg1	rates					595:599	high rates	590:599	high rates of gestational glucose intolerance	590:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	10	40	from	difucosyllactose	1949:1964	arg1	secretors					1969:1977	secretors	1969:1977	secretors	1969:1977	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	40	from	difucosyllactose	1949:1964	arg1	non-secretors					2019:2031	non-secretors	2019:2031	non-secretors	2019:2031	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	6	41	theme	linear	1067:1072	arg1	ɑ-diversity					1093:1103	ɑ-diversity	1093:1103	ɑ-diversity	1093:1103	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	41	theme	linear	1067:1072	arg1	models					1085:1090	linear regression models	1067:1090	linear regression models (ɑ-diversity)	1067:1104	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	7	42	theme	direct	1288:1293	arg1	breastfeeding					1295:1307	direct breastfeeding	1288:1307	direct breastfeeding	1288:1307	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	11	43	dep	composition	2233:2243	arg1	the					2219:2221	the	2219:2221	the	2219:2221	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	1	44	theme	bacteria	254:261	arg1	milk					190:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	44	theme	bacteria	254:261	arg1	source					205:210	a rich source	198:210	a rich source of human milk oligosaccharides (HMOs) and bacteria	198:261	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	10	45	from	disialyllacto-N-hexaose	1983:2005	arg1	secretors					1969:1977	secretors	1969:1977	secretors	1969:1977	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	45	from	disialyllacto-N-hexaose	1983:2005	arg1	non-secretors					2019:2031	non-secretors	2019:2031	non-secretors	2019:2031	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	9	46	theme	microbial	1792:1800	arg1	B-diversity					1802:1812	microbial B-diversity	1792:1812	microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036)	1792:1853	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	3	47	from	women	579:583	arg1	HMOs					502:505	HMOs	502:505	HMOs	502:505	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	47	from	women	579:583	arg1	milk					569:572	milk	569:572	milk from women with high rates of gestational glucose intolerance	569:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	47	from	women	579:583	arg1	composition					531:541	microbial community composition	511:541	microbial community composition	511:541	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	47	from	women	579:583	arg1	function					557:564	predicted function	547:564	predicted function in milk from women with high rates of gestational glucose intolerance	547:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	0	48	theme	Human	35:39	arg1	Milk					41:44	Human Milk	35:44	Human Milk	35:44	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	10	49	from	LNnT	2011:2014	arg1	secretors					1969:1977	secretors	1969:1977	secretors	1969:1977	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	49	from	LNnT	2011:2014	arg1	non-secretors					2019:2031	non-secretors	2019:2031	non-secretors	2019:2031	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	3	50	theme	glucose	616:622	arg1	intolerance					624:634	gestational glucose intolerance	604:634	gestational glucose intolerance	604:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	9	51	theme	UniFrac	1824:1830	arg1	P = 0.036					1844:1852	P = 0.036	1844:1852	P = 0.036	1844:1852	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	9	51	theme	UniFrac	1824:1830	arg1	R2 = 0.040					1832:1841	weighted UniFrac R2 = 0.040	1815:1841	weighted UniFrac R2 = 0.040	1815:1841	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	11	52	from	postpartum	2301:2310	arg1	composition					2233:2243	microbial composition	2223:2243	microbial composition	2223:2243	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	11	52	from	postpartum	2301:2310	arg1	functions					2269:2277	predicted microbial functions	2249:2277	predicted microbial functions	2249:2277	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	1	53	theme	rich	200:203	arg1	milk					190:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	53	theme	rich	200:203	arg1	source					205:210	a rich source	198:210	a rich source of human milk oligosaccharides (HMOs) and bacteria	198:261	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	7	54	theme	Prepregnancy	1247:1258	arg1	BMI					1260:1262	Prepregnancy BMI	1247:1262	Prepregnancy BMI	1247:1262	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	54	theme	Prepregnancy	1247:1258	arg1	RESULTS					1239:1245	RESULTS Prepregnancy BMI, race, and frequency	1239:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance,	1239:1349	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	4	55	dep	METHODS	637:643	arg1	This					645:648	This	645:648	This	645:648	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	10	56	theme	metabolism-	2107:2117	arg1	pathways					2141:2148	metabolism- and infection-related pathways	2107:2148	metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2107:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	56	theme	metabolism-	2107:2117	arg1	rate < 0.1					2169:2178	P-false discovery rate < 0.1	2151:2178	P-false discovery rate < 0.1	2151:2178	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	0	57	theme	Tolerance	162:170	arg1	Prevalence					119:128	a High Prevalence	112:128	a High Prevalence of Gestational Impaired Glucose Tolerance	112:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	2	58	theme	breast	319:324	arg1	microenvironment					326:341	the breast microenvironment	315:341	the breast microenvironment	315:341	It is unclear how these components interact within the breast microenvironment.					
34510198	8	59	theme	microbiota	1661:1670	arg1	diversity					1672:1680	microbiota diversity	1661:1680	microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors	1661:1737	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	8	59	theme	microbiota	1661:1670	arg1	index					1691:1695	Shannon index) [-1.7 (0.78); P = 0.029	1683:1720	Shannon index) [-1.7 (0.78); P = 0.029	1683:1720	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	1	60	theme	milk	221:224	arg1	oligosaccharides					226:241	human milk oligosaccharides	215:241	human milk oligosaccharides (HMOs)	215:248	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	60	theme	milk	221:224	arg1	HMOs					244:247	HMOs	244:247	HMOs	244:247	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	9	61	theme	KEGG	1859:1862	arg1	B-diversity					1873:1883	KEGG ortholog B-diversity	1859:1883	KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043)	1859:1919	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	3	62	theme	microbial	511:519	arg1	composition					531:541	microbial community composition	511:541	microbial community composition	511:541	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	8	63	dep	associated	1488:1497	arg1	P = 0.0082					1589:1598	P = 0.0082	1589:1598	P = 0.0082	1589:1598	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	6	64	theme	differential	1156:1167	arg1	models					1148:1153	Poisson regression models	1129:1153	Poisson regression models (differential abundance)	1129:1178	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	64	theme	differential	1156:1167	arg1	abundance					1169:1177	differential abundance	1156:1177	differential abundance	1156:1177	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	11	65	theme	mo	2298:2299	arg1	postpartum					2301:2310	3 mo postpartum	2296:2310	3 mo postpartum	2296:2310	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	7	66	theme	HMOs	1410:1413	arg1	number					1400:1405	a number	1398:1405	a number of HMOs among secretors and non-secretors	1398:1447	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	10	67	theme	infection-related	2123:2139	arg1	pathways					2141:2148	metabolism- and infection-related pathways	2107:2148	metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2107:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	67	theme	infection-related	2123:2139	arg1	rate < 0.1					2169:2178	P-false discovery rate < 0.1	2151:2178	P-false discovery rate < 0.1	2151:2178	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	3	68	theme	predicted	547:555	arg1	function					557:564	predicted function	547:564	predicted function in milk from women with high rates of gestational glucose intolerance	547:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	6	69	theme	microbial	1218:1226	arg1	models					1200:1205	general linear models	1185:1205	general linear models (predicted microbial function)	1185:1236	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	69	theme	microbial	1218:1226	arg1	function					1228:1235	predicted microbial function	1208:1235	predicted microbial function	1208:1235	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	70	theme	Poisson	1129:1135	arg1	models					1148:1153	Poisson regression models	1129:1153	Poisson regression models (differential abundance)	1129:1178	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	70	theme	Poisson	1129:1135	arg1	abundance					1169:1177	differential abundance	1156:1177	differential abundance	1156:1177	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	1	71	theme	Human	184:188	arg1	milk					190:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk	173:193	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	71	theme	Human	184:188	arg1	source					205:210	a rich source	198:210	a rich source of human milk oligosaccharides (HMOs) and bacteria	198:261	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	10	72	theme	discovery	2159:2167	arg1	pathways					2141:2148	metabolism- and infection-related pathways	2107:2148	metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2107:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	10	72	theme	discovery	2159:2167	arg1	rate < 0.1					2169:2178	P-false discovery rate < 0.1	2151:2178	P-false discovery rate < 0.1	2151:2178	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	0	73	theme	High	114:117	arg1	Prevalence					119:128	a High Prevalence	112:128	a High Prevalence of Gestational Impaired Glucose Tolerance	112:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	5	74	theme	V4-16S	851:856	arg1	sequencing					877:886	V4-16S ribosomal RNA gene sequencing	851:886	V4-16S ribosomal RNA gene sequencing	851:886	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	5	75	theme	microbiota	812:821	arg1	composition					823:833	Milk microbiota composition	807:833	Milk microbiota composition	807:833	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	10	76	theme	microbial	2078:2086	arg1	genes					2088:2092	predicted microbial genes	2068:2092	predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2068:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	6	77	theme	linear	1193:1198	arg1	models					1200:1205	general linear models	1185:1205	general linear models (predicted microbial function)	1185:1236	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	77	theme	linear	1193:1198	arg1	function					1228:1235	predicted microbial function	1208:1235	predicted microbial function	1208:1235	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	11	78	from	functions	2269:2277	arg1	milk					2288:2291	human milk	2282:2291	human milk	2282:2291	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	3	79	from	composition	531:541	arg1	women					579:583	women	579:583	women with high rates of gestational glucose intolerance	579:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	79	from	composition	531:541	arg1	milk					569:572	milk	569:572	milk from women with high rates of gestational glucose intolerance	569:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	0	80	theme	Impaired	145:152	arg1	Tolerance					162:170	Gestational Impaired Glucose Tolerance	133:170	Gestational Impaired Glucose Tolerance	133:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	7	81	theme	glucose	1330:1336	arg1	intolerance					1338:1348	gestational glucose intolerance	1318:1348	gestational glucose intolerance	1318:1348	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	4	82	theme	prospective	704:714	arg1	NCT01405547					730:740	NCT01405547	730:740	NCT01405547	730:740	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	4	82	theme	prospective	704:714	arg1	study					723:727	a previously completed prospective cohort study (NCT01405547)	681:741	a previously completed prospective cohort study (NCT01405547)	681:741	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	5	83	theme	ribosomal	858:866	arg1	sequencing					877:886	V4-16S ribosomal RNA gene sequencing	851:886	V4-16S ribosomal RNA gene sequencing	851:886	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	11	84	theme	microbial	2259:2267	arg1	functions					2269:2277	predicted microbial functions	2249:2277	predicted microbial functions	2249:2277	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	12	85	theme	Further	2313:2319	arg1	research					2321:2328	Further research	2313:2328	Further research	2313:2328	Further research is needed to investigate the role these relations play in maternal and infant health.					
34510198	4	86	theme	exploratory	657:667	arg1	analysis					669:676	an exploratory analysis	654:676	an exploratory analysis of a previously completed prospective cohort study (NCT01405547)	654:741	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	4	86	theme	exploratory	657:667	arg1	METHODS					637:643	METHODS	637:643	METHODS This	637:648	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	3	87	from	HMOs	502:505	arg1	women					579:583	women	579:583	women with high rates of gestational glucose intolerance	579:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	3	87	from	HMOs	502:505	arg1	milk					569:572	milk	569:572	milk from women with high rates of gestational glucose intolerance	569:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	5	88	theme	gene	872:875	arg1	sequencing					877:886	V4-16S ribosomal RNA gene sequencing	851:886	V4-16S ribosomal RNA gene sequencing	851:886	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	6	89	theme	status	983:988	arg1	phenotype					990:998	maternal secretor status phenotype	965:998	maternal secretor status phenotype	965:998	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	8	90	theme	microbial	1504:1512	arg1	Chao1					1524:1528	Chao1	1524:1528	Chao1	1524:1528	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	8	90	theme	microbial	1504:1512	arg1	richness					1514:1521	microbial richness	1504:1521	microbial richness (Chao1) among secretors [B-estimate (SE)	1504:1562	Fucosyllacto-N-hexaose was negatively associated with microbial richness (Chao1) among secretors [B-estimate (SE): -9.3 × 102 (3.4 × 102); P = 0.0082] and difucosyllacto-N-hexaose was negatively associated with microbiota diversity (Shannon index) [-1.7 (0.78); P = 0.029] among secretors.					
34510198	7	91	dep	RESULTS	1239:1245	arg1	race					1265:1268	race	1265:1268	race	1265:1268	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	91	dep	RESULTS	1239:1245	arg1	frequency					1275:1283	frequency	1275:1283	frequency	1275:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	91	dep	RESULTS	1239:1245	arg1	BMI					1260:1262	Prepregnancy BMI	1247:1262	Prepregnancy BMI	1247:1262	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	91	dep	RESULTS	1239:1245	arg1	RESULTS					1239:1245	RESULTS Prepregnancy BMI, race, and frequency	1239:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance,	1239:1349	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	6	92	theme	maternal	965:972	arg1	phenotype					990:998	maternal secretor status phenotype	965:998	maternal secretor status phenotype	965:998	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	7	93	theme	breastfeeding	1295:1307	arg1	race					1265:1268	race	1265:1268	race	1265:1268	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	93	theme	breastfeeding	1295:1307	arg1	frequency					1275:1283	frequency	1275:1283	frequency	1275:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	93	theme	breastfeeding	1295:1307	arg1	BMI					1260:1262	Prepregnancy BMI	1247:1262	Prepregnancy BMI	1247:1262	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	7	93	theme	breastfeeding	1295:1307	arg1	RESULTS					1239:1245	RESULTS Prepregnancy BMI, race, and frequency	1239:1283	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance,	1239:1349	RESULTS Prepregnancy BMI, race, and frequency of direct breastfeeding, but not gestational glucose intolerance, were found to be significantly associated with a number of HMOs among secretors and non-secretors.					
34510198	3	94	theme	maternal	421:428	arg1	characteristics					430:444	maternal characteristics	421:444	maternal characteristics	421:444	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	5	95	theme	high-throughput	906:920	arg1	HPLC					922:925	rapid high-throughput HPLC	900:925	rapid high-throughput HPLC	900:925	Milk microbiota composition was analyzed by V4-16S ribosomal RNA gene sequencing and HMOs by rapid high-throughput HPLC.					
34510198	4	96	theme	study	723:727	arg1	analysis					669:676	an exploratory analysis	654:676	an exploratory analysis of a previously completed prospective cohort study (NCT01405547)	654:741	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	4	96	theme	study	723:727	arg1	METHODS					637:643	METHODS	637:643	METHODS This	637:648	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	11	97	theme	CONCLUSIONS	2182:2192	arg1	HMOs					2194:2197	CONCLUSIONS HMOs	2182:2197	CONCLUSIONS HMOs	2182:2197	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	3	98	theme	intolerance	624:634	arg1	rates					595:599	high rates	590:599	high rates of gestational glucose intolerance	590:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	6	99	theme	regression	1074:1083	arg1	ɑ-diversity					1093:1103	ɑ-diversity	1093:1103	ɑ-diversity	1093:1103	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	6	99	theme	regression	1074:1083	arg1	models					1085:1090	linear regression models	1067:1090	linear regression models (ɑ-diversity)	1067:1104	Data were stratified and analyzed by maternal secretor status phenotype and associations between HMOs and microbiota were determined using linear regression models (ɑ-diversity), Adonis (B-diversity), Poisson regression models (differential abundance), and general linear models (predicted microbial function).					
34510198	3	100	theme	gestational	604:614	arg1	intolerance					624:634	gestational glucose intolerance	604:634	gestational glucose intolerance	604:634	OBJECTIVES The objectives were first, to investigate the association between maternal characteristics and HMOs, and second, to assess the association between HMOs and microbial community composition and predicted function in milk from women with high rates of gestational glucose intolerance.					
34510198	9	101	theme	weighted	1815:1822	arg1	P = 0.036					1844:1852	P = 0.036	1844:1852	P = 0.036	1844:1852	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	9	101	theme	weighted	1815:1822	arg1	R2 = 0.040					1832:1841	weighted UniFrac R2 = 0.040	1815:1841	weighted UniFrac R2 = 0.040	1815:1841	Lacto-N-neotetraose (LNnT) was associated with both microbial B-diversity (weighted UniFrac R2 = 0.040, P = 0.036) and KEGG ortholog B-diversity (Bray-Curtis R2 = 0.039, P = 0.043) in secretors.					
34510198	11	102	from	composition	2233:2243	arg1	milk					2288:2291	human milk	2282:2291	human milk	2282:2291	CONCLUSIONS HMOs are associated with the microbial composition and predicted microbial functions in human milk at 3 mo postpartum.					
34510198	4	103	theme	mo	792:793	arg1	postpartum					795:804	3 mo postpartum	790:804	3 mo postpartum	790:804	METHODS This was an exploratory analysis of a previously completed prospective cohort study (NCT01405547) where milk samples (n = 107) were collected at 3 mo postpartum.					
34510198	10	104	theme	predicted	2068:2076	arg1	genes					2088:2092	predicted microbial genes	2068:2092	predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1)	2068:2179	Additionally, difucosyllactose in secretors and disialyllacto-N-hexaose and LNnT in non-secretors were associated with enrichment of predicted microbial genes encoding for metabolism- and infection-related pathways (P-false discovery rate < 0.1).					
34510198	0	105	with	Women	101:105	arg1	Prevalence					119:128	a High Prevalence	112:128	a High Prevalence of Gestational Impaired Glucose Tolerance	112:170	Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.					
34510198	1	106	theme	human	215:219	arg1	oligosaccharides					226:241	human milk oligosaccharides	215:241	human milk oligosaccharides (HMOs)	215:248	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
34510198	1	106	theme	human	215:219	arg1	HMOs					244:247	HMOs	244:247	HMOs	244:247	BACKGROUND Human milk is a rich source of human milk oligosaccharides (HMOs) and bacteria.					
33302864	6	0	theme	transcriptomic	957:970	arg1	study					972:976	The transcriptomic study	953:976	The transcriptomic study	953:976	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	7	1	theme	particular	1290:1299	arg1	dependence					1301:1310	a particular dependence	1288:1310	ii) a particular dependence of the lactose onto the β-glucosidase regulation	1284:1359	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	2	theme	related	1191:1197	arg1	sub-networks					1178:1189	three sub-networks	1172:1189	three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth	1172:1428	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	9	3	theme	fine	1698:1701	arg1	regulation					1703:1712	a fine regulation	1696:1712	a fine regulation of the β-glucosidase	1696:1733	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	3	4	theme	network	598:604	arg1	overview					565:572	no overview	562:572	no overview of the whole regulation network	562:604	Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available.					
33302864	1	5	theme	generation	277:286	arg1	process					307:313	the second generation biofuel production process	266:313	the second generation biofuel production process	266:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	3	6	theme	whole	581:585	arg1	network					598:604	the whole regulation network	577:604	the whole regulation network	577:604	Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available.					
33302864	5	7	theme	sugar	899:903	arg1	mixtures					905:912	sugar mixtures	899:912	sugar mixtures	899:912	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	9	8	theme	β-glucosidase	1721:1733	arg1	co-orchestration					1598:1613	a co-orchestration	1596:1613	a co-orchestration of xyr1	1596:1621	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	8	theme	β-glucosidase	1721:1733	arg1	ace3					1633:1636	ace3	1633:1636	ace3	1633:1636	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	8	theme	β-glucosidase	1721:1733	arg1	regulation					1703:1712	a fine regulation	1696:1712	a fine regulation of the β-glucosidase	1696:1733	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	8	theme	β-glucosidase	1721:1733	arg1	clr2					1624:1627	clr2	1624:1627	clr2	1624:1627	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	8	theme	β-glucosidase	1721:1733	arg1	decrease					1741:1748	a decrease	1739:1748	a decrease of growth in favor of cellulase production	1739:1791	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	7	9	theme	BRANE	1145:1149	arg1	software					1155:1162	BRANE Cut software	1145:1162	BRANE Cut software	1145:1162	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	2	10	theme	lignocellulosic	330:344	arg1	substrates					346:355	lignocellulosic substrates	330:355	lignocellulosic substrates	330:355	Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei.					
33302864	1	11	theme	molecules	209:217	arg1	part					258:261	part	258:261	part of the second generation biofuel production process	258:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	1	11	theme	molecules	209:217	arg1	degradation					166:176	The degradation	162:176	The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose	162:253	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	6	12	theme	main	1013:1016	arg1	factors					1032:1038	the main transcription factors	1009:1038	the main transcription factors	1009:1038	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	0	13	theme	reesei	128:133	arg1	Rut-C30					142:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	1	14	theme	simpler	224:230	arg1	sugars					232:237	simpler sugars	224:237	simpler sugars such as glucose	224:253	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	1	14	theme	simpler	224:230	arg1	glucose					247:253	glucose	247:253	glucose	247:253	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	7	15	theme	regulatory	1114:1123	arg1	network					1125:1131	A gene regulatory network	1107:1131	A gene regulatory network built using BRANE Cut software	1107:1162	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	16	theme	positive	1207:1214	arg1	correlation					1216:1226	a positive correlation	1205:1226	i) a positive correlation between lactose concentration and cellulase production	1202:1281	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	6	17	theme	temporal	986:993	arg1	regulation					995:1004	a temporal regulation	984:1004	a temporal regulation of the main transcription factors	984:1038	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	9	18	from	clr2	1624:1627	arg1	favor					1763:1767	favor	1763:1767	favor	1763:1767	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	8	19	theme	pure	1528:1531	arg1	sources					1550:1556	pure and mixed carbon sources	1528:1556	pure and mixed carbon sources	1528:1556	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	7	20	theme	process	1411:1417	arg1	correlation					1216:1226	a positive correlation	1205:1226	i) a positive correlation between lactose concentration and cellulase production	1202:1281	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	20	theme	process	1411:1417	arg1	regulation					1381:1390	a negative regulation	1370:1390	a negative regulation of the development process	1370:1417	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	20	theme	process	1411:1417	arg1	dependence					1301:1310	a particular dependence	1288:1310	ii) a particular dependence of the lactose onto the β-glucosidase regulation	1284:1359	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	20	theme	process	1411:1417	arg1	growth					1423:1428	growth	1423:1428	growth	1423:1428	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	4	21	theme	missing	758:764	arg1	parts					766:770	missing parts	758:770	missing parts	758:770	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	8	22	theme	mixed	1537:1541	arg1	sources					1550:1556	pure and mixed carbon sources	1528:1556	pure and mixed carbon sources	1528:1556	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	8	23	from	effects	1517:1523	arg1	mode					1573:1576	a fed-batch mode	1561:1576	a fed-batch mode	1561:1576	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	5	24	theme	enzymatic	921:929	arg1	composition					940:950	the enzymatic cocktail composition	917:950	the enzymatic cocktail composition	917:950	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	9	25	from	co-orchestration	1598:1613	arg1	favor					1763:1767	favor	1763:1767	favor	1763:1767	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	6	26	theme	concentration	1054:1066	arg1	impact					1068:1073	a lactose concentration impact	1044:1073	a lactose concentration impact on the transcriptional profile	1044:1104	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	8	27	theme	sources	1550:1556	arg1	effects					1517:1523	the effects	1513:1523	the effects of pure and mixed carbon sources in a fed-batch mode	1513:1576	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	6	28	from	impact	1068:1073	arg1	profile					1098:1104	the transcriptional profile	1078:1104	the transcriptional profile	1078:1104	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	0	29	theme	network	77:83	arg1	analysis					85:92	a gene regulatory network analysis	59:92	a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30	59:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	9	30	from	growth	1753:1758	arg1	favor					1763:1767	favor	1763:1767	favor	1763:1767	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	4	31	theme	T.	790:791	arg1	Rut-C30					800:806	T. reesei Rut-C30	790:806	T. reesei Rut-C30	790:806	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	6	32	theme	factors	1032:1038	arg1	impact					1068:1073	a lactose concentration impact	1044:1073	a lactose concentration impact on the transcriptional profile	1044:1104	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	6	32	theme	factors	1032:1038	arg1	regulation					995:1004	a temporal regulation	984:1004	a temporal regulation of the main transcription factors	984:1038	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	4	33	theme	Rut-C30	800:806	arg1	network					779:785	the network	775:785	the network of T. reesei Rut-C30	775:806	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	2	34	theme	Trichoderma	425:435	arg1	reesei					437:442	the fungus Trichoderma reesei	414:442	the fungus Trichoderma reesei	414:442	Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei.					
33302864	0	35	theme	hyperproducing	101:114	arg1	Rut-C30					142:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	4	36	used	used	733:736	arg2	approach					637:644	A transcriptomic approach	620:644	A transcriptomic approach	620:644	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	3	37	theme	transcription	465:477	arg1	factors					479:485	transcription factors	465:485	transcription factors involved in the regulation of cellulase production	465:536	Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available.					
33302864	0	38	theme	Glucose-lactose	0:14	arg1	mixture					16:22	Glucose-lactose mixture	0:22	Glucose-lactose mixture	0:22	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	1	39	theme	biofuel	288:294	arg1	process					307:313	the second generation biofuel production process	266:313	the second generation biofuel production process	266:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	7	40	dep	dependence	1301:1310	arg1	ii					1284:1285	ii	1284:1285	ii	1284:1285	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	41	theme	negative	1372:1379	arg1	regulation					1381:1390	a negative regulation	1370:1390	a negative regulation of the development process	1370:1417	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	1	42	dep	BACKGROUND	151:160	arg1	part					258:261	part	258:261	part of the second generation biofuel production process	258:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	1	42	dep	BACKGROUND	151:160	arg1	degradation					166:176	The degradation	162:176	The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose	162:253	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	5	43	theme	hyperproducing	853:866	arg1	Rut-C30					845:851	the Rut-C30 hyperproducing	841:866	the Rut-C30 hyperproducing strain	841:873	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	1	44	theme	process	307:313	arg1	part					258:261	part	258:261	part of the second generation biofuel production process	258:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	1	44	theme	process	307:313	arg1	degradation					166:176	The degradation	162:176	The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose	162:253	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	9	45	from	ace3	1633:1636	arg1	favor					1763:1767	favor	1763:1767	favor	1763:1767	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	7	46	theme	lactose	1236:1242	arg1	concentration					1244:1256	lactose concentration	1236:1256	lactose concentration	1236:1256	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	0	47	theme	industry-like	33:45	arg1	conditions					47:56	industry-like conditions	33:56	industry-like conditions	33:56	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	6	48	theme	transcriptional	1082:1096	arg1	profile					1098:1104	the transcriptional profile	1078:1104	the transcriptional profile	1078:1104	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	0	49	theme	gene	61:64	arg1	analysis					85:92	a gene regulatory network analysis	59:92	a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30	59:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	7	50	theme	β-glucosidase	1336:1348	arg1	regulation					1350:1359	the β-glucosidase regulation	1332:1359	the β-glucosidase regulation	1332:1359	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	6	51	from	regulation	995:1004	arg1	profile					1098:1104	the transcriptional profile	1078:1104	the transcriptional profile	1078:1104	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	3	52	theme	production	527:536	arg1	regulation					503:512	the regulation	499:512	the regulation of cellulase production	499:536	Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available.					
33302864	0	53	dep	feeds	24:28	arg1	analysis					85:92	a gene regulatory network analysis	59:92	a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30	59:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	8	54	dep	CONCLUSIONS	1431:1441	arg1	work					1448:1451	This work	1443:1451	This work	1443:1451	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	8	54	dep	CONCLUSIONS	1431:1441	arg1	first					1460:1464	first	1460:1464	first	1460:1464	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	9	55	from	favor	1763:1767	arg1	co-orchestration					1598:1613	a co-orchestration	1596:1613	a co-orchestration of xyr1	1596:1621	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	55	from	favor	1763:1767	arg1	ace3					1633:1636	ace3	1633:1636	ace3	1633:1636	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	55	from	favor	1763:1767	arg1	regulation					1703:1712	a fine regulation	1696:1712	a fine regulation of the β-glucosidase	1696:1733	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	55	from	favor	1763:1767	arg1	clr2					1624:1627	clr2	1624:1627	clr2	1624:1627	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	55	from	favor	1763:1767	arg1	decrease					1741:1748	a decrease	1739:1748	a decrease of growth in favor of cellulase production	1739:1791	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	10	56	theme	cellulase-producing	1896:1914	arg1	strains					1916:1922	better cellulase-producing strains	1889:1922	better cellulase-producing strains in industry-like conditions	1889:1950	These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.					
33302864	4	57	theme	transcriptomic	622:635	arg1	approach					637:644	A transcriptomic approach	620:644	A transcriptomic approach	620:644	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	9	58	theme	cellulase	1642:1650	arg1	induction					1670:1678	cellulase and hemicellulase induction	1642:1678	cellulase and hemicellulase induction	1642:1678	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	3	59	theme	regulation	587:596	arg1	network					598:604	the whole regulation network	577:604	the whole regulation network	577:604	Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available.					
33302864	1	60	theme	second	270:275	arg1	process					307:313	the second generation biofuel production process	266:313	the second generation biofuel production process	266:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	10	61	theme	potential	1828:1836	arg1	targets					1838:1844	potential targets	1828:1844	potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions	1828:1950	These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.					
33302864	7	62	theme	Cut	1151:1153	arg1	software					1155:1162	BRANE Cut software	1145:1162	BRANE Cut software	1145:1162	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	5	63	theme	mixtures	905:912	arg1	impact					889:894	the impact	885:894	the impact of sugar mixtures on the enzymatic cocktail composition	885:950	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	1	64	theme	cellulose	181:189	arg1	molecules					209:217	cellulose and hemicellulose molecules	181:217	cellulose and hemicellulose molecules	181:217	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	5	65	dep	results	830:836	arg1	strain					868:873	the Rut-C30 hyperproducing strain	841:873	the Rut-C30 hyperproducing strain	841:873	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	2	66	theme	substrates	346:355	arg1	Hydrolysis					316:325	Hydrolysis	316:325	Hydrolysis of lignocellulosic substrates	316:355	Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei.					
33302864	1	67	theme	hemicellulose	195:207	arg1	molecules					209:217	cellulose and hemicellulose molecules	181:217	cellulose and hemicellulose molecules	181:217	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	6	68	theme	transcription	1018:1030	arg1	factors					1032:1038	the main transcription factors	1009:1038	the main transcription factors	1009:1038	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	4	69	theme	cellulase	708:716	arg1	induction					718:726	cellulase induction	708:726	cellulase induction	708:726	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	0	70	theme	strain	135:140	arg1	Rut-C30					142:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	0	71	from	analysis	85:92	arg1	Rut-C30					142:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	7	72	theme	gene	1109:1112	arg1	network					1125:1131	A gene regulatory network	1107:1131	A gene regulatory network built using BRANE Cut software	1107:1162	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	10	73	theme	genetic	1858:1864	arg1	engineering					1866:1876	further genetic engineering	1850:1876	further genetic engineering leading to better cellulase-producing strains in industry-like conditions	1850:1950	These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.					
33302864	9	74	theme	growth	1753:1758	arg1	co-orchestration					1598:1613	a co-orchestration	1596:1613	a co-orchestration of xyr1	1596:1621	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	74	theme	growth	1753:1758	arg1	ace3					1633:1636	ace3	1633:1636	ace3	1633:1636	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	74	theme	growth	1753:1758	arg1	regulation					1703:1712	a fine regulation	1696:1712	a fine regulation of the β-glucosidase	1696:1733	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	74	theme	growth	1753:1758	arg1	clr2					1624:1627	clr2	1624:1627	clr2	1624:1627	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	74	theme	growth	1753:1758	arg1	decrease					1741:1748	a decrease	1739:1748	a decrease of growth in favor of cellulase production	1739:1791	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	5	75	from	impact	889:894	arg1	composition					940:950	the enzymatic cocktail composition	917:950	the enzymatic cocktail composition	917:950	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	9	76	theme	cellulase	1772:1780	arg1	production					1782:1791	cellulase production	1772:1791	cellulase production	1772:1791	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	8	77	theme	carbon	1543:1548	arg1	sources					1550:1556	pure and mixed carbon sources	1528:1556	pure and mixed carbon sources	1528:1556	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	0	78	theme	regulatory	66:75	arg1	analysis					85:92	a gene regulatory network analysis	59:92	a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30	59:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	4	79	with	approach	637:644	arg1	mixtures					651:658	mixtures	651:658	mixtures of glucose and lactose	651:681	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	7	80	theme	development	1399:1409	arg1	process					1411:1417	the development process	1395:1417	the development process	1395:1417	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	5	81	theme	cocktail	931:938	arg1	composition					940:950	the enzymatic cocktail composition	917:950	the enzymatic cocktail composition	917:950	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	6	82	theme	lactose	1046:1052	arg1	impact					1068:1073	a lactose concentration impact	1044:1073	a lactose concentration impact on the transcriptional profile	1044:1104	The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile.					
33302864	8	83	theme	fed-batch	1563:1571	arg1	mode					1573:1576	a fed-batch mode	1561:1576	a fed-batch mode	1561:1576	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	7	84	dep	correlation	1216:1226	arg1	i					1202:1202	i	1202:1202	i	1202:1202	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	85	theme	cellulase	1262:1270	arg1	production					1272:1281	cellulase production	1262:1281	cellulase production	1262:1281	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	5	86	from	RESULTS	809:815	arg1	results					830:836	Experimental results	817:836	Experimental results	817:836	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	2	87	dep	enzymes	381:387	arg1	produced					389:396	produced	389:396	produced	389:396	Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei.					
33302864	2	87	dep	enzymes	381:387	arg1	secreted					402:409	secreted	402:409	secreted by the fungus Trichoderma reesei	402:442	Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei.					
33302864	0	88	theme	Trichoderma	116:126	arg1	Rut-C30					142:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	the hyperproducing Trichoderma reesei strain Rut-C30	97:148	Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.					
33302864	9	89	from	decrease	1741:1748	arg1	favor					1763:1767	favor	1763:1767	favor	1763:1767	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	4	90	theme	reesei	793:798	arg1	Rut-C30					800:806	T. reesei Rut-C30	790:806	T. reesei Rut-C30	790:806	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	2	91	theme	fungus	418:423	arg1	reesei					437:442	the fungus Trichoderma reesei	414:442	the fungus Trichoderma reesei	414:442	Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei.					
33302864	9	92	theme	xyr1	1618:1621	arg1	co-orchestration					1598:1613	a co-orchestration	1596:1613	a co-orchestration of xyr1	1596:1621	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	92	theme	xyr1	1618:1621	arg1	ace3					1633:1636	ace3	1633:1636	ace3	1633:1636	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	92	theme	xyr1	1618:1621	arg1	regulation					1703:1712	a fine regulation	1696:1712	a fine regulation of the β-glucosidase	1696:1733	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	92	theme	xyr1	1618:1621	arg1	clr2					1624:1627	clr2	1624:1627	clr2	1624:1627	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	9	92	theme	xyr1	1618:1621	arg1	decrease					1741:1748	a decrease	1739:1748	a decrease of growth in favor of cellulase production	1739:1791	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	5	93	theme	Rut-C30	845:851	arg1	strain					868:873	the Rut-C30 hyperproducing strain	841:873	the Rut-C30 hyperproducing strain	841:873	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	1	94	theme	production	296:305	arg1	process					307:313	the second generation biofuel production process	266:313	the second generation biofuel production process	266:313	BACKGROUND The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process.					
33302864	10	95	theme	industry-like	1927:1939	arg1	conditions					1941:1950	industry-like conditions	1927:1950	industry-like conditions	1927:1950	These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.					
33302864	3	96	theme	cellulase	517:525	arg1	production					527:536	cellulase production	517:536	cellulase production	517:536	Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available.					
33302864	5	97	theme	Experimental	817:828	arg1	results					830:836	Experimental results	817:836	Experimental results	817:836	RESULTS Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition.					
33302864	7	98	dep	regulation	1381:1390	arg1	iii					1365:1367	iii	1365:1367	iii	1365:1367	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	9	99	theme	hemicellulase	1656:1668	arg1	induction					1670:1678	cellulase and hemicellulase induction	1642:1678	cellulase and hemicellulase induction	1642:1678	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33302864	10	100	theme	further	1850:1856	arg1	engineering					1866:1876	further genetic engineering	1850:1876	further genetic engineering leading to better cellulase-producing strains in industry-like conditions	1850:1950	These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.					
33302864	7	101	theme	lactose	1319:1325	arg1	correlation					1216:1226	a positive correlation	1205:1226	i) a positive correlation between lactose concentration and cellulase production	1202:1281	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	101	theme	lactose	1319:1325	arg1	regulation					1381:1390	a negative regulation	1370:1390	a negative regulation of the development process	1370:1417	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	101	theme	lactose	1319:1325	arg1	dependence					1301:1310	a particular dependence	1288:1310	ii) a particular dependence of the lactose onto the β-glucosidase regulation	1284:1359	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	7	101	theme	lactose	1319:1325	arg1	growth					1423:1428	growth	1423:1428	growth	1423:1428	A gene regulatory network built using BRANE Cut software reveals three sub-networks related to i) a positive correlation between lactose concentration and cellulase production, ii) a particular dependence of the lactose onto the β-glucosidase regulation and iii) a negative regulation of the development process and growth.					
33302864	4	102	theme	glucose	663:669	arg1	mixtures					651:658	mixtures	651:658	mixtures of glucose and lactose	651:681	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	10	103	from	strains	1916:1922	arg1	conditions					1941:1950	industry-like conditions	1927:1950	industry-like conditions	1927:1950	These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains in industry-like conditions.					
33302864	4	104	theme	lactose	675:681	arg1	mixtures					651:658	mixtures	651:658	mixtures of glucose and lactose	651:681	A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.					
33302864	8	105	theme	transcriptomic	1482:1495	arg1	study					1497:1501	a transcriptomic study	1480:1501	a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode	1480:1576	CONCLUSIONS This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode.					
33302864	9	106	from	regulation	1703:1712	arg1	favor					1763:1767	favor	1763:1767	favor	1763:1767	Our study expose a co-orchestration of xyr1, clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production.					
33231337	0	0	theme	porcine	103:109	arg1	isolation					117:125	porcine islet isolation	103:125	porcine islet isolation	103:125	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	5	1	theme	EP-P	750:753	arg1	solution					768:775	EP solution	765:775	EP solution without 11R-p38I110	765:795	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	5	1	theme	EP-P	750:753	arg1	solution					755:762	EP-P solution	750:762	EP-P solution (EP solution without 11R-p38I110 )	750:797	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	10	2	theme	diabetic	1414:1421	arg1	mice					1423:1426	streptozotocin-induced diabetic mice	1391:1426	streptozotocin-induced diabetic mice	1391:1426	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	2	3	theme	inhibitor-containing	370:389	arg1	solution					396:403	an extracellular-type p38 inhibitor-containing (EP) solution	344:403	an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution	344:446	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	10	4	theme	islet	1490:1494	arg1	transplantation					1496:1510	islet transplantation	1490:1510	islet transplantation	1490:1510	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	6	5	theme	isolated	873:880	arg1	islets					882:887	isolated islets	873:887	isolated islets from each group	873:903	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	0	6	from	preservation	9:20	arg1	solution					69:76	extracellular-type p38 inhibitor-containing solution	25:76	extracellular-type p38 inhibitor-containing solution	25:76	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	10	7	theme	streptozotocin-induced	1391:1412	arg1	mice					1423:1426	streptozotocin-induced diabetic mice	1391:1426	streptozotocin-induced diabetic mice	1391:1426	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	2	8	theme	p38	366:368	arg1	solution					396:403	an extracellular-type p38 inhibitor-containing (EP) solution	344:403	an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution	344:446	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	11	9	with	CONCLUSIONS	1513:1523	arg1	function					1580:1587	EP solution preserved islet function	1552:1587	EP solution preserved islet function	1552:1587	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	11	9	with	CONCLUSIONS	1513:1523	arg1	solution					1609:1616	UW solution	1606:1616	UW solution	1606:1616	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	1	10	theme	mitogen-activated	273:289	arg1	MAPKs					308:312	MAPKs	308:312	MAPKs	308:312	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	10	theme	mitogen-activated	273:289	arg1	kinases					299:305	mitogen-activated protein kinases	273:305	mitogen-activated protein kinases (MAPKs)	273:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	12	11	theme	solution	1743:1750	arg1	composition					1724:1734	the composition	1720:1734	the inhibition of p38 activity as well as the composition of the solution	1678:1750	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	12	11	theme	solution	1743:1750	arg1	inhibition					1682:1691	the inhibition	1678:1691	the inhibition of p38 activity as well as the composition of the solution	1678:1750	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	7	12	theme	UW	1073:1074	arg1	group					1076:1080	the UW group	1069:1080	the UW group	1069:1080	RESULTS The islet yield before and after purification was significantly higher in the EP group than in the UW group.					
33231337	3	13	theme	high	511:514	arg1	composition					537:547	high sodium-low potassium composition	511:547	high sodium-low potassium composition	511:547	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	1	14	theme	protein	291:297	arg1	MAPKs					308:312	MAPKs	308:312	MAPKs	308:312	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	14	theme	protein	291:297	arg1	kinases					299:305	mitogen-activated protein kinases	273:305	mitogen-activated protein kinases (MAPKs)	273:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	0	15	theme	islet	111:115	arg1	isolation					117:125	porcine islet isolation	103:125	porcine islet isolation	103:125	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	6	16	from	number	853:858	arg1	group					899:903	each group	894:903	each group	894:903	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	3	17	theme	low	554:556	arg1	viscosity					558:566	low viscosity	554:566	low viscosity compared to UW solution	554:590	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	12	18	theme	solution	1640:1647	arg1	advantages					1623:1632	The advantages	1619:1632	The advantages of EP solution over UW solution	1619:1664	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	1	19	theme	kinases	299:305	arg1	group					264:268	the stress-activated group	243:268	the stress-activated group of mitogen-activated protein kinases (MAPKs)	243:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	19	theme	kinases	299:305	arg1	MAPKs					308:312	MAPKs	308:312	MAPKs	308:312	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	19	theme	kinases	299:305	arg1	kinases					299:305	mitogen-activated protein kinases	273:305	mitogen-activated protein kinases (MAPKs)	273:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	11	20	theme	preserved	1564:1572	arg1	function					1580:1587	EP solution preserved islet function	1552:1587	EP solution preserved islet function	1552:1587	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	5	21	theme	islet	809:813	arg1	isolation					815:823	then islet isolation	804:823	then islet isolation	804:823	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	3	22	theme	potassium	527:535	arg1	composition					537:547	high sodium-low potassium composition	511:547	high sodium-low potassium composition	511:547	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	12	23	theme	EP	1637:1638	arg1	solution					1640:1647	EP solution	1637:1647	EP solution	1637:1647	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	2	24	theme	organ	471:475	arg1	preservation					477:488	organ preservation	471:488	organ preservation	471:488	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	10	25	theme	islet	1364:1368	arg1	transplantation					1370:1384	islet transplantation	1364:1384	islet transplantation into streptozotocin-induced diabetic mice	1364:1426	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	10	26	from	preservation	1438:1449	arg1	solution					1457:1464	EP solution	1454:1464	EP solution	1454:1464	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	6	27	theme	islets	882:887	arg1	number					853:858	An optimized number	840:858	An optimized number (1500 IE) of isolated islets from each group	840:903	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	6	27	theme	islets	882:887	arg1	IE					866:867	1500 IE	861:867	1500 IE	861:867	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	12	28	theme	UW	1654:1655	arg1	solution					1657:1664	UW solution	1654:1664	UW solution	1654:1664	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	10	29	theme	transplantation	1496:1510	arg1	outcome					1479:1485	the outcome	1475:1485	the outcome of islet transplantation	1475:1510	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	11	30	theme	solution	1555:1562	arg1	function					1580:1587	EP solution preserved islet function	1552:1587	EP solution preserved islet function	1552:1587	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	11	31	dep	better	1589:1594	arg1	than					1596:1599	than	1596:1599	than	1596:1599	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	0	32	theme	Pancreas	0:7	arg1	preservation					9:20	Pancreas preservation	0:20	Pancreas preservation in extracellular-type p38 inhibitor-containing solution	0:76	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	6	33	theme	diabetic	951:958	arg1	mice					960:963	streptozotocin-induced diabetic mice	928:963	streptozotocin-induced diabetic mice	928:963	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	3	34	theme	UW	580:581	arg1	solution					583:590	UW solution	580:590	UW solution	580:590	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	8	35	theme	EP	1206:1207	arg1	solution					1209:1216	the EP solution	1202:1216	the EP solution	1202:1216	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	0	36	theme	extracellular-type	25:42	arg1	solution					69:76	extracellular-type p38 inhibitor-containing solution	25:76	extracellular-type p38 inhibitor-containing solution	25:76	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	6	37	theme	streptozotocin-induced	928:949	arg1	mice					960:963	streptozotocin-induced diabetic mice	928:963	streptozotocin-induced diabetic mice	928:963	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	5	38	theme	Porcine	703:709	arg1	pancreata					711:719	Porcine pancreata	703:719	Porcine pancreata	703:719	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	8	39	from	reduction	1230:1238	arg1	number					1247:1252	the number	1243:1252	the number of islets during culture	1243:1277	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	2	40	theme	UW	435:436	arg1	solution					439:446	Wisconsin (UW) solution	424:446	Wisconsin (UW) solution	424:446	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	9	41	theme	blot	1288:1291	arg1	analysis					1293:1300	Western blot analysis	1280:1300	Western blot analysis	1280:1300	Western blot analysis showed that p38 activation was attenuated by EP solution.					
33231337	7	42	theme	EP	1052:1053	arg1	group					1055:1059	the EP group	1048:1059	the EP group	1048:1059	RESULTS The islet yield before and after purification was significantly higher in the EP group than in the UW group.					
33231337	6	43	theme	optimized	843:851	arg1	number					853:858	An optimized number	840:858	An optimized number (1500 IE) of isolated islets from each group	840:903	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	6	43	theme	optimized	843:851	arg1	IE					866:867	1500 IE	861:867	1500 IE	861:867	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	1	44	theme	islet	192:196	arg1	isolation					198:206	islet isolation	192:206	islet isolation	192:206	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	7	45	dep	RESULTS	966:972	arg1	yield					984:988	The islet yield	974:988	The islet yield before and after purification	974:1018	RESULTS The islet yield before and after purification was significantly higher in the EP group than in the UW group.					
33231337	7	45	dep	RESULTS	966:972	arg1	higher					1038:1043	higher	1038:1043	higher	1038:1043	RESULTS The islet yield before and after purification was significantly higher in the EP group than in the UW group.					
33231337	1	46	theme	pancreas	166:173	arg1	preservation					175:186	pancreas preservation	166:186	pancreas preservation	166:186	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	0	47	theme	inhibitor-containing	48:67	arg1	solution					69:76	extracellular-type p38 inhibitor-containing solution	25:76	extracellular-type p38 inhibitor-containing solution	25:76	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	3	48	theme	EP	495:496	arg1	solution					498:505	The EP solution	491:505	The EP solution	491:505	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	10	49	theme	EP	1454:1455	arg1	solution					1457:1464	EP solution	1454:1464	EP solution	1454:1464	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	12	50	theme	p38	1696:1698	arg1	activity					1700:1707	p38 activity	1696:1707	p38 activity	1696:1707	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	2	51	theme	Wisconsin	424:432	arg1	solution					439:446	Wisconsin (UW) solution	424:446	Wisconsin (UW) solution	424:446	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	0	52	theme	p38	44:46	arg1	solution					69:76	extracellular-type p38 inhibitor-containing solution	25:76	extracellular-type p38 inhibitor-containing solution	25:76	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	5	53	theme	EP	765:766	arg1	solution					768:775	EP solution	765:775	EP solution without 11R-p38I110	765:795	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	5	53	theme	EP	765:766	arg1	solution					755:762	EP-P solution	750:762	EP-P solution (EP solution without 11R-p38I110 )	750:797	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	9	54	theme	Western	1280:1286	arg1	analysis					1293:1300	Western blot analysis	1280:1300	Western blot analysis	1280:1300	Western blot analysis showed that p38 activation was attenuated by EP solution.					
33231337	4	55	theme	p38	645:647	arg1	11R-p38I110					660:670	11R-p38I110	660:670	11R-p38I110	660:670	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	4	55	theme	p38	645:647	arg1	inhibitor					649:657	a recently developed p38 inhibitor	624:657	a recently developed p38 inhibitor (11R-p38I110 )	624:672	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	8	56	theme	EP	1173:1174	arg1	groups					1185:1190	the EP and EP-P groups	1169:1190	groups	1185:1190	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	8	57	theme	islet	1087:1091	arg1	yield					1093:1097	The islet yield	1083:1097	The islet yield before and after purification	1083:1127	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	8	57	theme	islet	1087:1091	arg1	different					1151:1159	different	1151:1159	different	1151:1159	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	2	58	theme	extracellular-type	347:364	arg1	solution					396:403	an extracellular-type p38 inhibitor-containing (EP) solution	344:403	an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution	344:446	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	8	59	theme	EP-P	1180:1183	arg1	groups					1185:1190	the EP and EP-P groups	1169:1190	groups	1185:1190	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	9	60	theme	p38	1314:1316	arg1	activation					1318:1327	p38 activation	1314:1327	p38 activation	1314:1327	Western blot analysis showed that p38 activation was attenuated by EP solution.					
33231337	3	61	contain	has	507:509	arg2	composition					537:547	high sodium-low potassium composition	511:547	high sodium-low potassium composition	511:547	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	3	61	contain	has	507:509	arg1	solution					498:505	The EP solution	491:505	The EP solution	491:505	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	10	62	theme	pancreas	1429:1436	arg1	preservation					1438:1449	pancreas preservation	1429:1449	pancreas preservation in EP solution	1429:1464	For islet transplantation into streptozotocin-induced diabetic mice, pancreas preservation in EP solution improved the outcome of islet transplantation.					
33231337	7	63	theme	islet	978:982	arg1	yield					984:988	The islet yield	974:988	The islet yield before and after purification	974:1018	RESULTS The islet yield before and after purification was significantly higher in the EP group than in the UW group.					
33231337	7	63	theme	islet	978:982	arg1	higher					1038:1043	higher	1038:1043	higher	1038:1043	RESULTS The islet yield before and after purification was significantly higher in the EP group than in the UW group.					
33231337	1	64	theme	islet	143:147	arg1	transplantation					149:163	islet transplantation	143:163	islet transplantation	143:163	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	11	65	theme	EP	1552:1553	arg1	function					1580:1587	EP solution preserved islet function	1552:1587	EP solution preserved islet function	1552:1587	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	4	66	contain	contains	615:622	arg1	solution					606:613	EP solution	603:613	EP solution	603:613	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	4	66	contain	contains	615:622	arg2	11R-p38I110					660:670	11R-p38I110	660:670	11R-p38I110	660:670	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	4	66	contain	contains	615:622	arg2	inhibitor					649:657	a recently developed p38 inhibitor	624:657	a recently developed p38 inhibitor (11R-p38I110 )	624:672	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	11	67	theme	islet	1574:1578	arg1	function					1580:1587	EP solution preserved islet function	1552:1587	EP solution preserved islet function	1552:1587	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	5	68	dep	METHODS	695:701	arg1	performed					829:837	performed	829:837	was performed	825:837	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	5	68	dep	METHODS	695:701	arg1	preserved					726:734	preserved	726:734	were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 )	721:797	METHODS Porcine pancreata were preserved in UW, EP, or EP-P solution (EP solution without 11R-p38I110 ), and then islet isolation was performed.					
33231337	6	69	from	group	899:903	arg1	number					853:858	An optimized number	840:858	An optimized number (1500 IE) of isolated islets from each group	840:903	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	6	69	from	group	899:903	arg1	IE					866:867	1500 IE	861:867	1500 IE	861:867	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	6	69	from	group	899:903	arg1	islets					882:887	isolated islets	873:887	isolated islets from each group	873:903	An optimized number (1500 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice.					
33231337	8	70	theme	islets	1257:1262	arg1	number					1247:1252	the number	1243:1252	the number of islets during culture	1243:1277	The islet yield before and after purification was not significantly different between the EP and EP-P groups; however, the EP solution prevented a reduction in the number of islets during culture.					
33231337	11	71	theme	UW	1606:1607	arg1	solution					1609:1616	UW solution	1606:1616	UW solution	1606:1616	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	12	72	theme	activity	1700:1707	arg1	composition					1724:1734	the composition	1720:1734	the inhibition of p38 activity as well as the composition of the solution	1678:1750	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	12	72	theme	activity	1700:1707	arg1	inhibition					1682:1691	the inhibition	1678:1691	the inhibition of p38 activity as well as the composition of the solution	1678:1750	The advantages of EP solution over UW solution may include the inhibition of p38 activity as well as the composition of the solution.					
33231337	4	73	theme	EP	603:604	arg1	solution					606:613	EP solution	603:613	EP solution	603:613	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	4	74	theme	developed	635:643	arg1	11R-p38I110					660:670	11R-p38I110	660:670	11R-p38I110	660:670	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	4	74	theme	developed	635:643	arg1	inhibitor					649:657	a recently developed p38 inhibitor	624:657	a recently developed p38 inhibitor (11R-p38I110 )	624:672	Moreover, EP solution contains a recently developed p38 inhibitor (11R-p38I110 ) from our laboratory.					
33231337	2	75	theme	EP	392:393	arg1	solution					396:403	an extracellular-type p38 inhibitor-containing (EP) solution	344:403	an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution	344:446	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	11	76	theme	Pancreas	1525:1532	arg1	preservation					1534:1545	Pancreas preservation	1525:1545	Pancreas preservation	1525:1545	CONCLUSIONS Pancreas preservation with EP solution preserved islet function better than with UW solution.					
33231337	1	77	theme	stress-activated	247:262	arg1	group					264:268	the stress-activated group	243:268	the stress-activated group of mitogen-activated protein kinases (MAPKs)	243:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	77	theme	stress-activated	247:262	arg1	MAPKs					308:312	MAPKs	308:312	MAPKs	308:312	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	77	theme	stress-activated	247:262	arg1	kinases					299:305	mitogen-activated protein kinases	273:305	mitogen-activated protein kinases (MAPKs)	273:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	0	78	theme	islet	87:91	arg1	yield					93:97	islet yield	87:97	islet yield for porcine islet isolation	87:125	Pancreas preservation in extracellular-type p38 inhibitor-containing solution improves islet yield for porcine islet isolation.					
33231337	2	79	with	solution	396:403	arg1	University					410:419	University	410:419	University	410:419	In this study, we evaluated an extracellular-type p38 inhibitor-containing (EP) solution with University of Wisconsin (UW) solution, the gold standard for organ preservation.					
33231337	9	80	theme	EP	1347:1348	arg1	solution					1350:1357	EP solution	1347:1357	EP solution	1347:1357	Western blot analysis showed that p38 activation was attenuated by EP solution.					
33231337	3	81	theme	sodium-low	516:525	arg1	composition					537:547	high sodium-low potassium composition	511:547	high sodium-low potassium composition	511:547	The EP solution has high sodium-low potassium composition with low viscosity compared to UW solution.					
33231337	1	82	theme	group	264:268	arg1	p38					217:219	p38	217:219	p38	217:219	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
33231337	1	82	theme	group	264:268	arg1	member					233:238	a member	231:238	a member of the stress-activated group of mitogen-activated protein kinases (MAPKs)	231:313	BACKGROUND For islet transplantation, pancreas preservation and islet isolation activate p38, which is a member of the stress-activated group of mitogen-activated protein kinases (MAPKs).					
32450324	4	0	theme	tensile	979:985	arg1	strength					987:994	The tensile strength	975:994	The tensile strength of the composite	975:1011	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	4	0	theme	tensile	979:985	arg1	higher					1030:1035	higher	1030:1035	higher	1030:1035	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	3	1	theme	PLGA	962:965	arg1	matrix					967:972	PLGA matrix	962:972	PLGA matrix	962:972	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	6	2	theme	combined-modification	1392:1412	arg1	method					1414:1419	the combined-modification method	1388:1419	the combined-modification method	1388:1419	The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility.					
32450324	3	3	theme	synergistic	859:869	arg1	effects					871:877	excellent synergistic effects	849:877	excellent synergistic effects	849:877	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	1	4	theme	second	172:177	arg1	biomacromolecule					201:216	the second most abundant natural biomacromolecule	168:216	the second most abundant natural biomacromolecule	168:216	Lignin is the second most abundant natural biomacromolecule.					
32450324	1	4	theme	second	172:177	arg1	Lignin					158:163	Lignin	158:163	Lignin	158:163	Lignin is the second most abundant natural biomacromolecule.					
32450324	3	5	with	bonding	936:942	arg1	matrix					967:972	PLGA matrix	962:972	PLGA matrix	962:972	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	5	6	theme	bone	1332:1335	arg1	cells					1354:1358	bone mesenchymal stem cells	1332:1358	bone mesenchymal stem cells	1332:1358	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	6	7	theme	degradation	1435:1445	arg1	behavior					1447:1454	good degradation behavior	1430:1454	good degradation behavior	1430:1454	The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility.					
32450324	6	8	theme	excellent	1491:1499	arg1	biocompatibility					1506:1521	excellent cell biocompatibility	1491:1521	excellent cell biocompatibility	1491:1521	The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility.					
32450324	5	9	theme	interaction	1309:1319	arg1	tests					1321:1325	interaction tests	1309:1325	interaction tests with bone mesenchymal stem cells	1309:1358	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	2	10	theme	dispersion	563:572	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	10	theme	dispersion	563:572	arg1	images					574:579	dispersion images	563:579	dispersion images	563:579	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	11	theme	scanning	601:608	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	11	theme	scanning	601:608	arg1	SEM					631:633	SEM	631:633	SEM	631:633	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	11	theme	scanning	601:608	arg1	microscope					619:628	scanning electron microscope	601:628	scanning electron microscope (SEM)	601:634	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	12	theme	electron	610:617	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	12	theme	electron	610:617	arg1	SEM					631:633	SEM	631:633	SEM	631:633	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	12	theme	electron	610:617	arg1	microscope					619:628	scanning electron microscope	601:628	scanning electron microscope (SEM)	601:634	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	4	13	theme	composite	1003:1011	arg1	strength					987:994	The tensile strength	975:994	The tensile strength of the composite	975:1011	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	4	13	theme	composite	1003:1011	arg1	higher					1030:1035	higher	1030:1035	higher	1030:1035	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	2	14	theme	scanning	650:657	arg1	DSC					672:674	DSC	672:674	DSC	672:674	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	14	theme	scanning	650:657	arg1	calorimeter					659:669	differential scanning calorimeter	637:669	differential scanning calorimeter (DSC)	637:675	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	14	theme	scanning	650:657	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	4	15	theme	%	1091:1091	arg1	amount					1076:1081	a n-HA addition amount	1060:1081	a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA	1060:1163	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	4	15	theme	%	1091:1091	arg1	%					1091:1091	15 wt%	1086:1091	15 wt%	1086:1091	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	1	16	theme	abundant	184:191	arg1	biomacromolecule					201:216	the second most abundant natural biomacromolecule	168:216	the second most abundant natural biomacromolecule	168:216	Lignin is the second most abundant natural biomacromolecule.					
32450324	1	16	theme	abundant	184:191	arg1	Lignin					158:163	Lignin	158:163	Lignin	158:163	Lignin is the second most abundant natural biomacromolecule.					
32450324	0	17	theme	combined-modification	2:22	arg1	method					24:29	A combined-modification method	0:29	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite	0:96	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.					
32450324	6	18	theme	good	1430:1433	arg1	behavior					1447:1454	good degradation behavior	1430:1454	good degradation behavior	1430:1454	The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility.					
32450324	2	19	theme	differential	637:648	arg1	DSC					672:674	DSC	672:674	DSC	672:674	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	19	theme	differential	637:648	arg1	calorimeter					659:669	differential scanning calorimeter	637:669	differential scanning calorimeter (DSC)	637:675	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	19	theme	differential	637:648	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	1	20	theme	natural	193:199	arg1	biomacromolecule					201:216	the second most abundant natural biomacromolecule	168:216	the second most abundant natural biomacromolecule	168:216	Lignin is the second most abundant natural biomacromolecule.					
32450324	1	20	theme	natural	193:199	arg1	Lignin					158:163	Lignin	158:163	Lignin	158:163	Lignin is the second most abundant natural biomacromolecule.					
32450324	2	21	theme	tensile	586:592	arg1	tests					594:598	the tensile tests	582:598	the tensile tests	582:598	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	21	theme	tensile	586:592	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	5	22	theme	mesenchymal	1337:1347	arg1	cells					1354:1358	bone mesenchymal stem cells	1332:1358	bone mesenchymal stem cells	1332:1358	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	0	23	dep	to	98:99	arg1	reinforce					101:109	reinforce	101:109	to reinforce poly(lactide-co-glycolide) for bone materials	98:155	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.					
32450324	5	24	theme	body	1248:1251	arg1	SBF					1260:1262	SBF	1260:1262	SBF	1260:1262	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	5	24	theme	body	1248:1251	arg1	fluid					1253:1257	simulated body fluid	1238:1257	simulated body fluid (SBF)	1238:1263	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	3	25	theme	appropriate	804:814	arg1	n-HA					834:837	the appropriate combined-modified n-HA	800:837	the appropriate combined-modified n-HA	800:837	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	0	26	theme	bone	142:145	arg1	materials					147:155	bone materials	142:155	bone materials	142:155	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.					
32450324	5	27	theme	stem	1349:1352	arg1	cells					1354:1358	bone mesenchymal stem cells	1332:1358	bone mesenchymal stem cells	1332:1358	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	3	28	theme	good	919:922	arg1	bonding					936:942	good interfacial bonding	919:942	good interfacial bonding between n-HA with PLGA matrix	919:972	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	2	29	dep	effect	348:353	arg1	reinforce					338:346	reinforce	338:346	reinforce	338:346	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	30	theme	gravimetric	535:545	arg1	TGA					557:559	TGA	557:559	TGA	557:559	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	30	theme	gravimetric	535:545	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	30	theme	gravimetric	535:545	arg1	analysis					547:554	thermal gravimetric analysis	527:554	thermal gravimetric analysis (TGA)	527:560	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	5	31	dep	in	1180:1181	arg1	vitro					1183:1187	vitro	1183:1187	vitro	1183:1187	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	7	32	theme	surface-modified	1613:1628	arg1	n-HA					1630:1633	surface-modified n-HA	1613:1633	surface-modified n-HA as bone materials	1613:1651	This study may offer an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials.					
32450324	4	33	theme	addition	1067:1074	arg1	amount					1076:1081	a n-HA addition amount	1060:1081	a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA	1060:1163	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	4	33	theme	addition	1067:1074	arg1	%					1091:1091	15 wt%	1086:1091	15 wt%	1086:1091	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	2	34	theme	infrared	437:444	arg1	transformation					422:435	Fourier transformation	414:435	Fourier transformation infrared (FTIR)	414:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	0	35	theme	β-cyclodextrin	48:61	arg1	method					24:29	A combined-modification method	0:29	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite	0:96	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.					
32450324	4	36	theme	singled-modified	1143:1158	arg1	n-HA					1160:1163	the singled-modified n-HA	1139:1163	the singled-modified n-HA	1139:1163	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	2	37	theme	thermal	527:533	arg1	TGA					557:559	TGA	557:559	TGA	557:559	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	37	theme	thermal	527:533	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	37	theme	thermal	527:533	arg1	analysis					547:554	thermal gravimetric analysis	527:554	thermal gravimetric analysis (TGA)	527:560	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	7	38	theme	PLGA-based	1577:1586	arg1	composites					1588:1597	PLGA-based composites	1577:1597	PLGA-based composites reinforced by surface-modified n-HA as bone materials	1577:1651	This study may offer an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials.					
32450324	4	39	theme	n-HA	1062:1065	arg1	amount					1076:1081	a n-HA addition amount	1060:1081	a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA	1060:1163	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	4	39	theme	n-HA	1062:1065	arg1	%					1091:1091	15 wt%	1086:1091	15 wt%	1086:1091	The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA.					
32450324	0	40	theme	carboxymethyl	34:46	arg1	β-cyclodextrin					48:61	carboxymethyl β-cyclodextrin	34:61	carboxymethyl β-cyclodextrin	34:61	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.					
32450324	5	41	with	tests	1321:1325	arg1	cells					1354:1358	bone mesenchymal stem cells	1332:1358	bone mesenchymal stem cells	1332:1358	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	6	42	theme	cell	1501:1504	arg1	biocompatibility					1506:1521	excellent cell biocompatibility	1491:1521	excellent cell biocompatibility	1491:1521	The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility.					
32450324	2	43	theme	Fourier	414:420	arg1	transformation					422:435	Fourier transformation	414:435	Fourier transformation infrared (FTIR)	414:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	0	44	theme	lignin	67:72	arg1	method					24:29	A combined-modification method	0:29	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite	0:96	A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.					
32450324	5	45	theme	degradation	1189:1199	arg1	behavior					1201:1208	in vitro degradation behavior	1180:1208	in vitro degradation behavior	1180:1208	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	2	46	theme	diffraction	460:470	arg1	pattern					472:478	X-ray diffraction pattern	454:478	X-ray diffraction pattern (XRD)	454:484	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	46	theme	diffraction	460:470	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	46	theme	diffraction	460:470	arg1	XRD					481:483	XRD	481:483	XRD	481:483	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	47	dep	infrared	437:444	arg1	FTIR					447:450	FTIR	447:450	FTIR	447:450	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	3	48	theme	interfacial	924:934	arg1	bonding					936:942	good interfacial bonding	919:942	good interfacial bonding between n-HA with PLGA matrix	919:972	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	2	49	theme	lignin	768:773	arg1	singled-modification					733:752	the singled-modification	729:752	the singled-modification of CM-β-CD or lignin	729:773	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	7	50	theme	important	1548:1556	arg1	guidance					1558:1565	an important guidance	1545:1565	an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials	1545:1651	This study may offer an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials.					
32450324	2	51	theme	new	221:223	arg1	surface-modification					225:244	A new surface-modification	219:244	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM),	219:715	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	3	52	theme	combined-modified	816:832	arg1	n-HA					834:837	the appropriate combined-modified n-HA	800:837	the appropriate combined-modified n-HA	800:837	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
32450324	2	53	theme	carboxymethyl	280:292	arg1	CM-β-CD					310:316	CM-β-CD	310:316	CM-β-CD	310:316	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	53	theme	carboxymethyl	280:292	arg1	β-cyclodextrin					294:307	carboxymethyl β-cyclodextrin	280:307	carboxymethyl β-cyclodextrin (CM-β-CD)	280:317	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	5	54	theme	cell	1276:1279	arg1	response					1281:1288	their cell response	1270:1288	their cell response	1270:1288	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	6	55	theme	apatite	1460:1466	arg1	deposition					1468:1477	apatite deposition	1460:1477	apatite deposition	1460:1477	The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility.					
32450324	2	56	theme	CM-β-CD	757:763	arg1	singled-modification					733:752	the singled-modification	729:752	the singled-modification of CM-β-CD or lignin	729:773	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	7	57	theme	bone	1638:1641	arg1	materials					1643:1651	bone materials	1638:1651	bone materials	1638:1651	This study may offer an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials.					
32450324	2	58	theme	electron	500:507	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	58	theme	electron	500:507	arg1	TEM					521:523	TEM	521:523	TEM	521:523	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	58	theme	electron	500:507	arg1	POM					711:713	POM	711:713	POM	711:713	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	58	theme	electron	500:507	arg1	microscopy					509:518	transmission electron microscopy	487:518	transmission electron microscopy (TEM)	487:524	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	59	theme	optical	691:697	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	59	theme	optical	691:697	arg1	microscopy					699:708	polarized optical microscopy	681:708	polarized optical microscopy	681:708	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	5	60	theme	simulated	1238:1246	arg1	SBF					1260:1262	SBF	1260:1262	SBF	1260:1262	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	5	60	theme	simulated	1238:1246	arg1	fluid					1253:1257	simulated body fluid	1238:1257	simulated body fluid (SBF)	1238:1263	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	5	61	theme	in	1180:1181	arg1	behavior					1201:1208	in vitro degradation behavior	1180:1208	in vitro degradation behavior	1180:1208	Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells.					
32450324	2	62	theme	transmission	487:498	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	62	theme	transmission	487:498	arg1	TEM					521:523	TEM	521:523	TEM	521:523	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	62	theme	transmission	487:498	arg1	POM					711:713	POM	711:713	POM	711:713	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	62	theme	transmission	487:498	arg1	microscopy					509:518	transmission electron microscopy	487:518	transmission electron microscopy (TEM)	487:524	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	63	theme	polarized	681:689	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	63	theme	polarized	681:689	arg1	microscopy					699:708	polarized optical microscopy	681:708	polarized optical microscopy	681:708	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	64	theme	X-ray	454:458	arg1	pattern					472:478	X-ray diffraction pattern	454:478	X-ray diffraction pattern (XRD)	454:484	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	64	theme	X-ray	454:458	arg1	effect					348:353	effect	348:353	effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR)	348:451	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	2	64	theme	X-ray	454:458	arg1	XRD					481:483	XRD	481:483	XRD	481:483	A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin.					
32450324	3	65	theme	excellent	849:857	arg1	effects					871:877	excellent synergistic effects	849:877	excellent synergistic effects	849:877	The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix.					
34775878	10	0	theme	p	1389:1389	arg1	.49					1393:1395	S = -44.5, p = .49	1378:1395	.49	1393:1395	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	0	theme	p	1389:1389	arg1	centrifugation					1362:1375	centrifugation	1362:1375	centrifugation (S = -44.5, p = .49)	1362:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	12	1	theme	preterm	1585:1591	arg1	infants					1593:1599	feeding preterm infants	1577:1599	feeding preterm infants	1577:1599	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	2	2	theme	feed	139:142	arg1	infants					152:158	feed preterm infants	139:158	feed preterm infants in neonatal intensive care units	139:191	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	7	3	theme	processes	799:807	arg1	results					751:757	the results	747:757	the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk	747:872	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	12	4	with	product	1653:1659	arg1	components					1707:1716	no non-human components	1694:1716	no non-human components	1694:1716	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	12	4	with	product	1653:1659	arg1	density					1682:1688	higher nutrient density	1666:1688	higher nutrient density	1666:1688	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	8	5	theme	WakeMed	904:910	arg1	Mothers					912:918	WakeMed Mothers'	904:919	WakeMed Mothers' Milk Bank	904:929	Donor milk was obtained from WakeMed Mothers' Milk Bank.					
34775878	11	6	theme	osmolality	1483:1492	arg1	Academy					1449:1455	the American Academy	1436:1455	the American Academy of Pediatrics recommended osmolality for infant feeding	1436:1511	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	11	7	theme	30	1401:1402	arg1	%					1403:1403	%	1403:1403	%	1403:1403	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	7	8	theme	lactose	783:789	arg1	processes					799:807	lactose removal processes	783:807	lactose removal processes	783:807	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	9	dep	METHODS	723:729	arg1	investigated					734:745	investigated	734:745	investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk	734:872	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	12	10	theme	bank	1560:1563	arg1	setting					1565:1571	a milk bank setting	1553:1571	a milk bank setting for feeding preterm infants	1553:1599	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	7	11	theme	reduction	769:777	arg1	results					751:757	the results	747:757	the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk	747:872	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	3	12	theme	Preterm	236:242	arg1	infants					244:250	Preterm infants	236:250	Preterm infants	236:250	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	5	13	with	milk	508:511	arg1	density					537:543	increased nutrient density	518:543	increased nutrient density	518:543	Therefore, there is a need for human milk with increased nutrient density.					
34775878	6	14	theme	high	661:664	arg1	osmolality					666:675	high osmolality	661:675	high osmolality	661:675	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	4	15	theme	higher	399:404	arg1	protein					406:412	higher protein and energy requirements	399:436	protein	406:412	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	9	16	from	0	1114:1114	arg1	centrifugation					1076:1089	refrigerated centrifugation	1063:1089	refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls	1063:1190	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	10	17	dep	RESULTS	1193:1199	arg1	reduction					1215:1223	A significant reduction	1201:1223	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01)	1193:1282	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	12	18	theme	milk	1555:1558	arg1	setting					1565:1571	a milk bank setting	1553:1571	a milk bank setting for feeding preterm infants	1553:1599	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	0	19	theme	Preterm	55:61	arg1	Infants					63:69	Preterm Infants	55:69	Preterm Infants	55:69	Processing Human Milk to Increase Nutrient Density for Preterm Infants.					
34775878	2	20	theme	donor	212:216	arg1	milk					218:221	donor milk	212:221	donor milk	212:221	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	10	21	from	centrifugation	1362:1375	arg1	loss					1352:1355	a significant protein loss	1330:1355	a significant protein loss from centrifugation (S = -44.5, p = .49)	1330:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	3	22	theme	human	278:282	arg1	milk					284:287	human milk	278:287	human milk	278:287	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	11	23	theme	infant	1498:1503	arg1	feeding					1505:1511	infant feeding	1498:1511	infant feeding	1498:1511	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	5	24	theme	nutrient	528:535	arg1	density					537:543	increased nutrient density	518:543	increased nutrient density	518:543	Therefore, there is a need for human milk with increased nutrient density.					
34775878	9	25	dep	0	1114:1114	arg1	compared					1152:1159	compared	1152:1159	compared to the composition of controls	1152:1190	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	9	25	dep	0	1114:1114	arg1	separated					1138:1146	separated	1138:1146	separated	1138:1146	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	3	26	theme	caloric	304:310	arg1	density					312:318	the lower caloric density	294:318	the lower caloric density of donor milk	294:332	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	4	27	theme	stomach	453:459	arg1	capacity					461:468	a limited stomach capacity	443:468	a limited stomach capacity	443:468	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	10	28	theme	protein	1344:1350	arg1	loss					1352:1355	a significant protein loss	1330:1355	a significant protein loss from centrifugation (S = -44.5, p = .49)	1330:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	5	29	theme	human	502:506	arg1	milk					508:511	human milk	502:511	human milk with increased nutrient density	502:543	Therefore, there is a need for human milk with increased nutrient density.					
34775878	1	30	theme	BACKGROUND	72:81	arg1	milk					89:92	BACKGROUND Human milk	72:92	BACKGROUND Human milk	72:92	BACKGROUND Human milk is the optimal food for newborns.					
34775878	1	30	theme	BACKGROUND	72:81	arg1	food					109:112	the optimal food	97:112	the optimal food for newborns	97:125	BACKGROUND Human milk is the optimal food for newborns.					
34775878	3	31	theme	milk	329:332	arg1	density					312:318	the lower caloric density	294:318	the lower caloric density of donor milk	294:332	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	11	32	theme	%	1407:1407	arg1	reduction					1416:1424	A 30%-40% volume reduction	1399:1424	A 30%-40% volume reduction	1399:1424	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	0	33	theme	Processing	0:9	arg1	Milk					17:20	Processing Human Milk	0:20	Processing Human Milk	0:20	Processing Human Milk to Increase Nutrient Density for Preterm Infants.					
34775878	10	34	theme	lactose	1228:1234	arg1	reduction					1215:1223	A significant reduction	1201:1223	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01)	1193:1282	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	3	35	theme	preterm	349:355	arg1	growth					364:369	preterm infant growth needs	349:375	preterm infant growth needs	349:375	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	6	36	theme	higher	595:600	arg1	caloric					602:608	a higher caloric	593:608	a higher caloric	593:608	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	2	37	theme	preterm	144:150	arg1	infants					152:158	feed preterm infants	139:158	feed preterm infants in neonatal intensive care units	139:191	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	10	38	theme	=	1380:1380	arg1	.49					1393:1395	S = -44.5, p = .49	1378:1395	.49	1393:1395	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	38	theme	=	1380:1380	arg1	centrifugation					1362:1375	centrifugation	1362:1375	centrifugation (S = -44.5, p = .49)	1362:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	39	theme	S	1237:1237	arg1	lactose					1228:1234	lactose	1228:1234	lactose (S < .0001)	1228:1246	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	39	theme	S	1237:1237	arg1	<					1239:1239	S < .0001	1237:1245	S < .0001	1237:1245	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	12	40	theme	higher	1666:1671	arg1	density					1682:1688	higher nutrient density	1666:1688	higher nutrient density	1666:1688	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	8	41	theme	Donor	875:879	arg1	milk					881:884	Donor milk	875:884	Donor milk	875:884	Donor milk was obtained from WakeMed Mothers' Milk Bank.					
34775878	2	42	theme	intensive	172:180	arg1	units					187:191	neonatal intensive care units	163:191	neonatal intensive care units	163:191	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	11	43	theme	American	1440:1447	arg1	Academy					1449:1455	the American Academy	1436:1455	the American Academy of Pediatrics recommended osmolality for infant feeding	1436:1511	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	12	44	theme	low-cost	1623:1630	arg1	CONCLUSION					1514:1523	CONCLUSION	1514:1523	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants	1514:1599	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	12	44	theme	low-cost	1623:1630	arg1	process					1632:1638	a simple and low-cost process	1610:1638	a simple and low-cost process to achieve a product with higher nutrient density and no non-human components	1610:1716	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	10	45	theme	=	1391:1391	arg1	.49					1393:1395	S = -44.5, p = .49	1378:1395	.49	1393:1395	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	45	theme	=	1391:1391	arg1	centrifugation					1362:1375	centrifugation	1362:1375	centrifugation (S = -44.5, p = .49)	1362:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	11	46	theme	recommended	1471:1481	arg1	osmolality					1483:1492	Pediatrics recommended osmolality	1460:1492	Pediatrics recommended osmolality	1460:1492	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	11	47	theme	%	1403:1403	arg1	reduction					1416:1424	A 30%-40% volume reduction	1399:1424	A 30%-40% volume reduction	1399:1424	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	9	48	theme	lactose	1095:1101	arg1	removal					1103:1109	lactose removal	1095:1109	lactose removal	1095:1109	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	10	49	theme	=	1266:1266	arg1	p					1275:1275	S = -211.5 p < .01	1264:1281	S = -211.5 p < .01	1264:1281	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	49	theme	=	1266:1266	arg1	osmolality					1252:1261	osmolality	1252:1261	osmolality (S = -211.5 p < .01)	1252:1282	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	8	50	theme	Milk	921:924	arg1	Bank					926:929	WakeMed Mothers' Milk Bank	904:929	WakeMed Mothers' Milk Bank	904:929	Donor milk was obtained from WakeMed Mothers' Milk Bank.					
34775878	6	51	theme	RESEARCH	546:553	arg1	AIM					555:557	RESEARCH AIM	546:557	RESEARCH AIM	546:557	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	9	52	theme	°C.	1116:1118	arg1	Supernatants					1120:1131	°C. Supernatants	1116:1131	°C. Supernatants	1116:1131	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	7	53	theme	removal	791:797	arg1	processes					799:807	lactose removal processes	783:807	lactose removal processes	783:807	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	54	from	results	751:757	arg1	osmolality					834:843	osmolality	834:843	osmolality	834:843	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	54	from	results	751:757	arg1	viscosity					850:858	viscosity	850:858	viscosity	850:858	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	54	from	results	751:757	arg1	protein					825:831	protein	825:831	protein	825:831	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	54	from	results	751:757	arg1	lactose					816:822	lactose	816:822	lactose	816:822	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	12	55	theme	feeding	1577:1583	arg1	infants					1593:1599	feeding preterm infants	1577:1599	feeding preterm infants	1577:1599	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	6	56	theme	low	705:707	arg1	temperatures					709:720	low temperatures	705:720	low temperatures	705:720	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	12	57	theme	human	1539:1543	arg1	milk					1545:1548	human milk	1539:1548	human milk	1539:1548	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	7	58	theme	volume	762:767	arg1	reduction					769:777	volume reduction	762:777	volume reduction	762:777	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	1	59	theme	optimal	101:107	arg1	milk					89:92	BACKGROUND Human milk	72:92	BACKGROUND Human milk	72:92	BACKGROUND Human milk is the optimal food for newborns.					
34775878	1	59	theme	optimal	101:107	arg1	food					109:112	the optimal food	97:112	the optimal food for newborns	97:125	BACKGROUND Human milk is the optimal food for newborns.					
34775878	3	60	theme	better	257:262	arg1	tolerance					264:272	better tolerance	257:272	better tolerance	257:272	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	4	61	theme	Preterm	378:384	arg1	infants					386:392	Preterm infants	378:392	Preterm infants	378:392	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	9	62	theme	evaporative	951:961	arg1	condensation					963:974	evaporative condensation	951:974	evaporative condensation	951:974	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	12	63	theme	simple	1612:1617	arg1	CONCLUSION					1514:1523	CONCLUSION	1514:1523	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants	1514:1599	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	12	63	theme	simple	1612:1617	arg1	process					1632:1638	a simple and low-cost process	1610:1638	a simple and low-cost process to achieve a product with higher nutrient density and no non-human components	1610:1716	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	9	64	theme	controls	1183:1190	arg1	composition					1168:1178	the composition	1164:1178	the composition of controls	1164:1190	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	4	65	theme	limited	445:451	arg1	capacity					461:468	a limited stomach capacity	443:468	a limited stomach capacity	443:468	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	6	66	theme	donor	574:578	arg1	milk					580:583	donor milk	574:583	donor milk	574:583	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	10	67	theme	concentrated	1304:1315	arg1	milk					1317:1320	the concentrated milk	1300:1320	the concentrated milk	1300:1320	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	6	68	theme	osmolality	666:675	arg1	effects					650:656	side effects	645:656	side effects of high osmolality	645:675	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	4	69	theme	energy	418:423	arg1	requirements					425:436	higher protein and energy requirements	399:436	requirements	425:436	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	10	70	theme	significant	1203:1213	arg1	reduction					1215:1223	A significant reduction	1201:1223	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01)	1193:1282	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	6	71	theme	side	645:648	arg1	effects					650:656	side effects	645:656	side effects of high osmolality	645:675	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	3	72	contain	have	252:255	arg1	infants					244:250	Preterm infants	236:250	Preterm infants	236:250	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	3	72	contain	have	252:255	arg2	tolerance					264:272	better tolerance	257:272	better tolerance	257:272	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	3	73	theme	lower	298:302	arg1	density					312:318	the lower caloric density	294:318	the lower caloric density of donor milk	294:332	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	10	74	theme	significant	1332:1342	arg1	loss					1352:1355	a significant protein loss	1330:1355	a significant protein loss from centrifugation (S = -44.5, p = .49)	1330:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	9	75	theme	=	1003:1003	arg1	N					1001:1001	N = 36	1001:1006	N = 36	1001:1006	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	9	75	theme	=	1003:1003	arg1	samples					992:998	samples	992:998	samples (N = 36)	992:1007	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	0	76	theme	Human	11:15	arg1	Milk					17:20	Processing Human Milk	0:20	Processing Human Milk	0:20	Processing Human Milk to Increase Nutrient Density for Preterm Infants.					
34775878	7	77	theme	milk	869:872	arg1	osmolality					834:843	osmolality	834:843	osmolality	834:843	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	77	theme	milk	869:872	arg1	viscosity					850:858	viscosity	850:858	viscosity	850:858	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	77	theme	milk	869:872	arg1	protein					825:831	protein	825:831	protein	825:831	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	7	77	theme	milk	869:872	arg1	lactose					816:822	lactose	816:822	lactose	816:822	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	1	78	theme	Human	83:87	arg1	milk					89:92	BACKGROUND Human milk	72:92	BACKGROUND Human milk	72:92	BACKGROUND Human milk is the optimal food for newborns.					
34775878	1	78	theme	Human	83:87	arg1	food					109:112	the optimal food	97:112	the optimal food for newborns	97:125	BACKGROUND Human milk is the optimal food for newborns.					
34775878	6	79	theme	protein	614:620	arg1	density					622:628	protein density	614:628	protein density	614:628	RESEARCH AIM To concentrate donor milk to have a higher caloric and protein density while avoiding side effects of high osmolality by precipitating lactose at low temperatures.					
34775878	5	80	theme	increased	518:526	arg1	density					537:543	increased nutrient density	518:543	increased nutrient density	518:543	Therefore, there is a need for human milk with increased nutrient density.					
34775878	3	81	theme	donor	323:327	arg1	milk					329:332	donor milk	323:332	donor milk	323:332	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	2	82	theme	neonatal	163:170	arg1	units					187:191	neonatal intensive care units	163:191	neonatal intensive care units	163:191	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	0	83	theme	Nutrient	34:41	arg1	Density					43:49	Nutrient Density	34:49	Nutrient Density for Preterm Infants	34:69	Processing Human Milk to Increase Nutrient Density for Preterm Infants.					
34775878	11	84	dep	recommended	1471:1481	arg1	Pediatrics					1460:1469	Pediatrics	1460:1469	Pediatrics	1460:1469	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	12	85	theme	non-human	1697:1705	arg1	components					1707:1716	no non-human components	1694:1716	no non-human components	1694:1716	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	3	86	dep	growth	364:369	arg1	needs					371:375	needs	371:375	needs	371:375	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	3	87	theme	infant	357:362	arg1	growth					364:369	preterm infant growth needs	349:375	preterm infant growth needs	349:375	Preterm infants have better tolerance for human milk, but the lower caloric density of donor milk might not meet preterm infant growth needs.					
34775878	11	88	theme	volume	1409:1414	arg1	reduction					1416:1424	A 30%-40% volume reduction	1399:1424	A 30%-40% volume reduction	1399:1424	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	4	89	contain	have	394:397	arg2	protein					406:412	higher protein and energy requirements	399:436	protein	406:412	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	4	89	contain	have	394:397	arg1	infants					386:392	Preterm infants	378:392	Preterm infants	378:392	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	4	89	contain	have	394:397	arg2	requirements					425:436	higher protein and energy requirements	399:436	requirements	425:436	Preterm infants have higher protein and energy requirements with a limited stomach capacity.					
34775878	10	90	theme	-44.5	1382:1386	arg1	.49					1393:1395	S = -44.5, p = .49	1378:1395	.49	1393:1395	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	90	theme	-44.5	1382:1386	arg1	centrifugation					1362:1375	centrifugation	1362:1375	centrifugation (S = -44.5, p = .49)	1362:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	11	91	theme	-40	1404:1406	arg1	%					1407:1407	%	1407:1407	%	1407:1407	A 30%-40% volume reduction is within the American Academy of Pediatrics recommended osmolality for infant feeding.					
34775878	12	92	theme	nutrient	1673:1680	arg1	density					1682:1688	higher nutrient density	1666:1688	higher nutrient density	1666:1688	CONCLUSION Concentrating human milk in a milk bank setting for feeding preterm infants might be a simple and low-cost process to achieve a product with higher nutrient density and no non-human components.					
34775878	7	93	theme	human	863:867	arg1	milk					869:872	human milk	863:872	human milk	863:872	METHODS We investigated the results of volume reduction and lactose removal processes on the lactose, protein, osmolality, and viscosity of human milk.					
34775878	9	94	theme	refrigerated	1063:1074	arg1	centrifugation					1076:1089	refrigerated centrifugation	1063:1089	refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls	1063:1190	Homogenization and evaporative condensation were applied to samples (N = 36) before they were stored frozen overnight, followed by refrigerated centrifugation for lactose removal at 0 °C. Supernatants were separated and compared to the composition of controls.					
34775878	10	95	theme	S	1378:1378	arg1	.49					1393:1395	S = -44.5, p = .49	1378:1395	.49	1393:1395	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	95	theme	S	1378:1378	arg1	centrifugation					1362:1375	centrifugation	1362:1375	centrifugation (S = -44.5, p = .49)	1362:1396	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	2	96	theme	care	182:185	arg1	units					187:191	neonatal intensive care units	163:191	neonatal intensive care units	163:191	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	10	97	theme	S	1264:1264	arg1	p					1275:1275	S = -211.5 p < .01	1264:1281	S = -211.5 p < .01	1264:1281	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	10	97	theme	S	1264:1264	arg1	osmolality					1252:1261	osmolality	1252:1261	osmolality (S = -211.5 p < .01)	1252:1282	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
34775878	2	98	from	infants	152:158	arg1	units					187:191	neonatal intensive care units	163:191	neonatal intensive care units	163:191	Choices to feed preterm infants in neonatal intensive care units are mother's milk, donor milk, or formula.					
34775878	10	99	theme	osmolality	1252:1261	arg1	reduction					1215:1223	A significant reduction	1201:1223	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01)	1193:1282	RESULTS A significant reduction of lactose (S < .0001) and osmolality (S = -211.5 p < .01) was achieved in the concentrated milk without a significant protein loss from centrifugation (S = -44.5, p = .49).					
32784105	5	0	theme	cancer	702:707	arg1	model					718:722	a 3D breast cancer in vitro model	690:722	a 3D breast cancer in vitro model	690:722	Herein, a 3D breast cancer in vitro model has been proposed.					
32784105	6	1	link	crosslinked	772:782	arg1	matrices					800:807	crosslinked and lyophilized matrices	772:807	crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network	772:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	13	2	theme	ECM	1774:1776	arg1	composition					1778:1788	ECM composition	1774:1788	ECM composition	1774:1788	Further studies will be needed to elucidate the influence of ECM composition on EP efficiency.					
32784105	4	3	theme	three-dimensional	538:554	arg1	spheroids					586:594	spheroids	586:594	spheroids	586:594	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	4	3	theme	three-dimensional	538:554	arg1	cultures					615:622	hydrogel-based cultures	600:622	hydrogel-based cultures	600:622	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	4	3	theme	three-dimensional	538:554	arg1	models					570:575	various three-dimensional (3D) in vitro models	530:575	various three-dimensional (3D) in vitro models	530:575	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	0	4	theme	in	67:68	arg1	model					76:80	3D in vitro model	64:80	3D in vitro model for electroporation	64:100	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	9	5	theme	3D	1224:1225	arg1	cultures					1227:1234	both 2D and 3D cultures	1212:1234	both 2D and 3D cultures	1212:1234	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	11	6	theme	viability	1594:1602	arg1	terms					1545:1549	terms	1545:1549	terms of both cell membrane permeabilization and viability	1545:1602	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	2	7	theme	cell	320:323	arg1	cultures					325:332	cell suspension and adherent cell cultures	291:332	cultures	325:332	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	13	8	theme	composition	1778:1788	arg1	influence					1761:1769	the influence	1757:1769	the influence of ECM composition on EP efficiency	1757:1805	Further studies will be needed to elucidate the influence of ECM composition on EP efficiency.					
32784105	4	9	theme	various	530:536	arg1	spheroids					586:594	spheroids	586:594	spheroids	586:594	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	4	9	theme	various	530:536	arg1	cultures					615:622	hydrogel-based cultures	600:622	hydrogel-based cultures	600:622	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	4	9	theme	various	530:536	arg1	models					570:575	various three-dimensional (3D) in vitro models	530:575	various three-dimensional (3D) in vitro models	530:575	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	11	10	theme	HA	1529:1530	arg1	cultures					1532:1539	2D and HA cultures	1522:1539	2D and HA cultures	1522:1539	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	9	11	from	5 kHz	1271:1275	arg1	pulses					1261:1266	8 voltage pulses	1251:1266	8 voltage pulses at 5 kHz with different amplitude	1251:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	9	11	from	5 kHz	1271:1275	arg1	sequence					1239:1246	a sequence	1237:1246	a sequence of 8 voltage pulses at 5 kHz with different amplitude	1237:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	6	12	theme	HCC1954	743:749	arg1	cells					751:755	HCC1954 cells	743:755	HCC1954 cells	743:755	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	9	13	with	5 kHz	1271:1275	arg1	amplitude					1292:1300	different amplitude	1282:1300	different amplitude	1282:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	4	14	theme	tumour	657:662	arg1	microenvironment					664:679	the tumour microenvironment	653:679	the tumour microenvironment	653:679	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	8	15	theme	cancer	1118:1123	arg1	cells					1125:1129	cancer cells	1118:1129	cancer cells	1118:1129	Cultures were characterized by spheroids surrounded by ECM produced by cancer cells as demonstrated by collagen1a1 and laminin B1 transcripts.					
32784105	1	16	theme	Nowadays	103:110	arg1	electroporation					113:127	Nowadays, electroporation	103:127	electroporation	113:127	Nowadays, electroporation (EP) represents a promising method for the intracellular delivery of anticancer drugs.					
32784105	1	16	theme	Nowadays	103:110	arg1	EP					130:131	EP	130:131	EP	130:131	Nowadays, electroporation (EP) represents a promising method for the intracellular delivery of anticancer drugs.					
32784105	7	17	theme	adhesion	1031:1038	arg1	motif					1040:1044	laminin derived IKVAV adhesion motif	1009:1044	laminin derived IKVAV adhesion motif	1009:1044	Herein, SAPs were functionalized with laminin derived IKVAV adhesion motif.					
32784105	2	18	theme	EP	247:248	arg1	efficiency					250:259	the EP efficiency	243:259	the EP efficiency	243:259	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	1	19	theme	promising	147:155	arg1	method					157:162	a promising method	145:162	a promising method for the intracellular delivery of anticancer drugs	145:213	Nowadays, electroporation (EP) represents a promising method for the intracellular delivery of anticancer drugs.					
32784105	6	20	theme	hyaluronic	821:830	arg1	acid					832:835	hyaluronic acid	821:835	hyaluronic acid (HA)	821:840	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	6	20	theme	hyaluronic	821:830	arg1	HA					838:839	HA	838:839	HA	838:839	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	6	21	theme	fibrous	925:931	arg1	structure					933:941	a fibrous structure	923:941	a fibrous structure mimicking collagen network	923:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	10	22	theme	Cell	1339:1342	arg1	sensitivity					1344:1354	Cell sensitivity	1339:1354	Cell sensitivity to EP	1339:1360	Cell sensitivity to EP seemed to be modulated by the presence of ECM and the different cell organization.					
32784105	11	23	from	terms	1545:1549	arg1	sensitive					1490:1498	sensitive	1490:1498	sensitive	1490:1498	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	9	24	theme	different	1282:1290	arg1	amplitude					1292:1300	different amplitude	1282:1300	different amplitude	1282:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	0	25	theme	cancer	7:12	arg1	cells					14:18	Breast cancer cells	0:18	Breast cancer cells	0:18	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	5	26	theme	in	709:710	arg1	model					718:722	a 3D breast cancer in vitro model	690:722	a 3D breast cancer in vitro model	690:722	Herein, a 3D breast cancer in vitro model has been proposed.					
32784105	7	27	theme	derived	1017:1023	arg1	motif					1040:1044	laminin derived IKVAV adhesion motif	1009:1044	laminin derived IKVAV adhesion motif	1009:1044	Herein, SAPs were functionalized with laminin derived IKVAV adhesion motif.					
32784105	5	28	theme	3D	692:693	arg1	model					718:722	a 3D breast cancer in vitro model	690:722	a 3D breast cancer in vitro model	690:722	Herein, a 3D breast cancer in vitro model has been proposed.					
32784105	10	29	theme	different	1416:1424	arg1	organization					1431:1442	the different cell organization	1412:1442	the different cell organization	1412:1442	Cell sensitivity to EP seemed to be modulated by the presence of ECM and the different cell organization.					
32784105	0	30	theme	Breast	0:5	arg1	cells					14:18	Breast cancer cells	0:18	Breast cancer cells	0:18	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	7	31	theme	IKVAV	1025:1029	arg1	motif					1040:1044	laminin derived IKVAV adhesion motif	1009:1044	laminin derived IKVAV adhesion motif	1009:1044	Herein, SAPs were functionalized with laminin derived IKVAV adhesion motif.					
32784105	0	32	dep	in	67:68	arg1	vitro					70:74	vitro	70:74	vitro	70:74	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	9	33	theme	voltage	1253:1259	arg1	pulses					1261:1266	8 voltage pulses	1251:1266	8 voltage pulses at 5 kHz with different amplitude	1251:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	10	34	theme	ECM	1404:1406	arg1	organization					1431:1442	the different cell organization	1412:1442	the different cell organization	1412:1442	Cell sensitivity to EP seemed to be modulated by the presence of ECM and the different cell organization.					
32784105	10	34	theme	ECM	1404:1406	arg1	presence					1392:1399	the presence	1388:1399	the presence of ECM	1388:1406	Cell sensitivity to EP seemed to be modulated by the presence of ECM and the different cell organization.					
32784105	1	35	theme	intracellular	172:184	arg1	delivery					186:193	the intracellular delivery	168:193	the intracellular delivery of anticancer drugs	168:213	Nowadays, electroporation (EP) represents a promising method for the intracellular delivery of anticancer drugs.					
32784105	11	36	theme	2D	1522:1523	arg1	cultures					1532:1539	2D and HA cultures	1522:1539	2D and HA cultures	1522:1539	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	9	37	theme	pulses	1261:1266	arg1	sequence					1239:1246	a sequence	1237:1246	a sequence of 8 voltage pulses at 5 kHz with different amplitude	1237:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	5	38	dep	in	709:710	arg1	vitro					712:716	vitro	712:716	vitro	712:716	Herein, a 3D breast cancer in vitro model has been proposed.					
32784105	11	39	theme	permeabilization	1573:1588	arg1	terms					1545:1549	terms	1545:1549	terms of both cell membrane permeabilization and viability	1545:1602	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	2	40	theme	conditions	377:386	arg1	representative					347:360	representative	347:360	representative	347:360	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	0	41	theme	hyaluronic	29:38	arg1	scaffolds					51:59	hyaluronic acid-based scaffolds	29:59	hyaluronic acid-based scaffolds as 3D in vitro model for electroporation	29:100	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	7	42	link	derived	1017:1023	arg1	motif					1040:1044	laminin derived IKVAV adhesion motif	1009:1044	laminin derived IKVAV adhesion motif	1009:1044	Herein, SAPs were functionalized with laminin derived IKVAV adhesion motif.					
32784105	13	43	theme	Further	1713:1719	arg1	studies					1721:1727	Further studies	1713:1727	Further studies	1713:1727	Further studies will be needed to elucidate the influence of ECM composition on EP efficiency.					
32784105	11	44	from	sensitive	1490:1498	arg1	terms					1545:1549	terms	1545:1549	terms of both cell membrane permeabilization and viability	1545:1602	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	4	45	theme	hydrogel-based	600:613	arg1	cultures					615:622	hydrogel-based cultures	600:622	hydrogel-based cultures	600:622	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	8	46	dep	collagen1a1	1150:1160	arg1	transcripts					1177:1187	B1 transcripts	1174:1187	B1 transcripts	1174:1187	Cultures were characterized by spheroids surrounded by ECM produced by cancer cells as demonstrated by collagen1a1 and laminin B1 transcripts.					
32784105	4	47	dep	three-dimensional	538:554	arg1	in					561:562	in	561:562	in	561:562	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	4	47	dep	three-dimensional	538:554	arg1	3D					557:558	3D	557:558	3D	557:558	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	2	48	dep	in	369:370	arg1	vivo					372:375	vivo	372:375	vivo	372:375	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	9	49	theme	plate	1322:1326	arg1	electrode					1328:1336	a plate electrode	1320:1336	a plate electrode	1320:1336	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	2	50	theme	in	369:370	arg1	conditions					377:386	the in vivo conditions	365:386	the in vivo conditions	365:386	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	8	51	theme	B1	1174:1175	arg1	transcripts					1177:1187	B1 transcripts	1174:1187	B1 transcripts	1174:1187	Cultures were characterized by spheroids surrounded by ECM produced by cancer cells as demonstrated by collagen1a1 and laminin B1 transcripts.					
32784105	10	52	theme	cell	1426:1429	arg1	organization					1431:1442	the different cell organization	1412:1442	the different cell organization	1412:1442	Cell sensitivity to EP seemed to be modulated by the presence of ECM and the different cell organization.					
32784105	11	53	theme	membrane	1564:1571	arg1	permeabilization					1573:1588	cell membrane permeabilization	1559:1588	cell membrane permeabilization	1559:1588	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	12	54	theme	EP	1701:1702	arg1	studies					1704:1710	EP studies	1701:1710	EP studies	1701:1710	Collectively, our results suggest that HA-IKVAV cultures may represent an interesting model for EP studies.					
32784105	2	55	theme	adherent	311:318	arg1	cultures					325:332	cell suspension and adherent cell cultures	291:332	cultures	325:332	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	0	56	theme	acid-based	40:49	arg1	scaffolds					51:59	hyaluronic acid-based scaffolds	29:59	hyaluronic acid-based scaffolds as 3D in vitro model for electroporation	29:100	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	6	57	theme	self-assembling	866:880	arg1	peptides					882:889	ionic complementary self-assembling peptides	846:889	ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network	846:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	6	57	theme	self-assembling	866:880	arg1	SAPs					892:895	SAPs	892:895	SAPs	892:895	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	9	58	theme	2D	1217:1218	arg1	cultures					1227:1234	both 2D and 3D cultures	1212:1234	both 2D and 3D cultures	1212:1234	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32784105	6	59	theme	crosslinked	772:782	arg1	matrices					800:807	crosslinked and lyophilized matrices	772:807	crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network	772:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	6	60	theme	lyophilized	788:798	arg1	matrices					800:807	crosslinked and lyophilized matrices	772:807	crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network	772:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	13	61	theme	EP	1793:1794	arg1	efficiency					1796:1805	EP efficiency	1793:1805	EP efficiency	1793:1805	Further studies will be needed to elucidate the influence of ECM composition on EP efficiency.					
32784105	6	62	theme	complementary	852:864	arg1	peptides					882:889	ionic complementary self-assembling peptides	846:889	ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network	846:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	6	62	theme	complementary	852:864	arg1	SAPs					892:895	SAPs	892:895	SAPs	892:895	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	11	63	theme	cell	1559:1562	arg1	permeabilization					1573:1588	cell membrane permeabilization	1559:1588	cell membrane permeabilization	1559:1588	Indeed, cells cultured on HA-IKVAV were more sensitive than those treated in 2D and HA cultures, in terms of both cell membrane permeabilization and viability.					
32784105	12	64	theme	HA-IKVAV	1644:1651	arg1	cultures					1653:1660	HA-IKVAV cultures	1644:1660	HA-IKVAV cultures	1644:1660	Collectively, our results suggest that HA-IKVAV cultures may represent an interesting model for EP studies.					
32784105	1	65	theme	drugs	209:213	arg1	delivery					186:193	the intracellular delivery	168:193	the intracellular delivery of anticancer drugs	168:213	Nowadays, electroporation (EP) represents a promising method for the intracellular delivery of anticancer drugs.					
32784105	4	66	dep	in	561:562	arg1	vitro					564:568	vitro	564:568	vitro	564:568	So, various three-dimensional (3D) in vitro models, such as spheroids and hydrogel-based cultures, have been proposed to mimic the tumour microenvironment.					
32784105	13	67	from	influence	1761:1769	arg1	efficiency					1796:1805	EP efficiency	1793:1805	EP efficiency	1793:1805	Further studies will be needed to elucidate the influence of ECM composition on EP efficiency.					
32784105	12	68	theme	interesting	1679:1689	arg1	model					1691:1695	an interesting model	1676:1695	an interesting model for EP studies	1676:1710	Collectively, our results suggest that HA-IKVAV cultures may represent an interesting model for EP studies.					
32784105	2	69	theme	cell	291:294	arg1	suspension					296:305	cell suspension and adherent cell cultures	291:332	suspension	296:305	To setting up the process, the EP efficiency is usually evaluated by using cell suspension and adherent cell cultures that are not representative of the in vivo conditions.					
32784105	6	70	theme	collagen	953:960	arg1	network					962:968	collagen network	953:968	collagen network	953:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	0	71	theme	3D	64:65	arg1	model					76:80	3D in vitro model	64:80	3D in vitro model for electroporation	64:100	Breast cancer cells grown on hyaluronic acid-based scaffolds as 3D in vitro model for electroporation.					
32784105	1	72	theme	anticancer	198:207	arg1	drugs					209:213	anticancer drugs	198:213	anticancer drugs	198:213	Nowadays, electroporation (EP) represents a promising method for the intracellular delivery of anticancer drugs.					
32784105	7	73	dep	derived	1017:1023	arg1	laminin					1009:1015	laminin	1009:1015	laminin	1009:1015	Herein, SAPs were functionalized with laminin derived IKVAV adhesion motif.					
32784105	3	74	theme	extracellular	421:433	arg1	ECM					443:445	ECM	443:445	ECM	443:445	Indeed, cells are surrounded by extracellular matrix (ECM) whose composition and physical characteristics are different for each tissue.					
32784105	3	74	theme	extracellular	421:433	arg1	matrix					435:440	extracellular matrix	421:440	extracellular matrix (ECM) whose composition and physical characteristics are different for each tissue	421:523	Indeed, cells are surrounded by extracellular matrix (ECM) whose composition and physical characteristics are different for each tissue.					
32784105	3	75	theme	physical	470:477	arg1	characteristics					479:493	physical characteristics	470:493	physical characteristics	470:493	Indeed, cells are surrounded by extracellular matrix (ECM) whose composition and physical characteristics are different for each tissue.					
32784105	5	76	theme	breast	695:700	arg1	model					718:722	a 3D breast cancer in vitro model	690:722	a 3D breast cancer in vitro model	690:722	Herein, a 3D breast cancer in vitro model has been proposed.					
32784105	6	77	theme	ionic	846:850	arg1	peptides					882:889	ionic complementary self-assembling peptides	846:889	ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network	846:968	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	6	77	theme	ionic	846:850	arg1	SAPs					892:895	SAPs	892:895	SAPs	892:895	HCC1954 cells were seeded on crosslinked and lyophilized matrices composed of hyaluronic acid (HA) and ionic complementary self-assembling peptides (SAPs) already known to provide a fibrous structure mimicking collagen network.					
32784105	9	78	from	sequence	1239:1246	arg1	5 kHz					1271:1275	5 kHz	1271:1275	5 kHz with different amplitude	1271:1300	EP was carried out on both 2D and 3D cultures: a sequence of 8 voltage pulses at 5 kHz with different amplitude was applied using a plate electrode.					
32278610	4	0	dep	METHODS	541:547	arg1	followed					581:588	followed	581:588	followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1	581:784	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	4	1	theme	different	716:724	arg1	concentrations					726:739	different concentrations	716:739	different concentrations	716:739	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	8	2	from	changes	1166:1172	arg1	mass					1188:1191	total body mass	1177:1191	total body mass	1177:1191	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	1	3	dep	BACKGROUND	94:103	arg1	highlighted					150:160	highlighted	150:160	highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases	150:277	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	7	4	theme	Body	894:897	arg1	composition					903:913	Body fat composition	894:913	Body fat composition	894:913	Body fat composition has been evaluated and ex vivo aortic vasomotor function has been investigated with a pharmacological approach.					
32278610	9	5	theme	significant	1288:1298	arg1	dysfunction					1321:1331	a significant vascular endothelial dysfunction	1286:1331	a significant vascular endothelial dysfunction compared to other groups	1286:1356	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	9	6	theme	vascular	1300:1307	arg1	dysfunction					1321:1331	a significant vascular endothelial dysfunction	1286:1331	a significant vascular endothelial dysfunction compared to other groups	1286:1356	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	1	7	theme	artificial	188:197	arg1	sweeteners					199:208	artificial sweeteners	188:208	artificial sweeteners (AS) consumption	188:225	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	9	8	theme	Acceptable	1233:1242	arg1	Intake					1250:1255	Acceptable Daily Intake	1233:1255	Acceptable Daily Intake (ADI) concentration	1233:1275	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	9	8	theme	Acceptable	1233:1242	arg1	ADI					1258:1260	ADI	1258:1260	ADI	1258:1260	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	8	9	theme	body	1183:1186	arg1	mass					1188:1191	total body mass	1177:1191	total body mass	1177:1191	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	3	10	from	effect	421:426	arg1	marker					506:511	an early marker	497:511	an early marker for cardiovascular disease	497:538	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	3	10	from	effect	421:426	arg1	composition					462:472	body composition	457:472	body composition	457:472	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	3	10	from	effect	421:426	arg1	function					487:494	vascular function	478:494	vascular function	478:494	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	8	11	theme	rats	1064:1067	arg1	groups					1043:1048	Both groups	1038:1048	Both groups of AS-treated rats	1038:1067	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	8	11	theme	rats	1064:1067	arg1	rats					1064:1067	AS-treated rats	1053:1067	AS-treated rats	1053:1067	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	0	12	dep	reactivity	50:59	arg1	results					74:80	Preliminary results	62:80	endothelial vascular reactivity: Preliminary results in rodents	29:91	Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents.					
32278610	4	13	theme	standard	600:607	arg1	diet					609:612	a 10-week standard diet	590:612	a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1	590:784	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	8	14	theme	total	1177:1181	arg1	mass					1188:1191	total body mass	1177:1191	total body mass	1177:1191	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	8	15	theme	AS-treated	1053:1062	arg1	rats					1064:1067	AS-treated rats	1053:1067	AS-treated rats	1053:1067	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	7	16	theme	vasomotor	953:961	arg1	function					963:970	ex vivo aortic vasomotor function	938:970	ex vivo aortic vasomotor function	938:970	Body fat composition has been evaluated and ex vivo aortic vasomotor function has been investigated with a pharmacological approach.					
32278610	3	17	theme	early	500:504	arg1	marker					506:511	an early marker	497:511	an early marker for cardiovascular disease	497:538	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	3	17	theme	early	500:504	arg1	composition					462:472	body composition	457:472	body composition	457:472	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	4	18	theme	10-week	592:598	arg1	diet					609:612	a 10-week standard diet	590:612	a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1	590:784	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	5	19	from	15 mg.kg-1	831:840	arg1	intake					814:819	high intake	809:819	high intake at 15 and 15 mg.kg-1	809:840	day-1, respectively or high intake at 15 and 15 mg.kg-1.					
32278610	5	19	from	15 mg.kg-1	831:840	arg1	day-1					786:790	day-1	786:790	day-1	786:790	day-1, respectively or high intake at 15 and 15 mg.kg-1.					
32278610	10	20	from	increase	1443:1450	arg1	risk					1471:1474	cardiometabolic risk	1455:1474	cardiometabolic risk	1455:1474	These results are interesting because they will help to better explain the observed increase in cardiometabolic risk.					
32278610	4	21	from	2 mg.kg-1	776:784	arg1	consumption					755:765	moderate consumption	746:765	moderate consumption at 1 and 2 mg.kg-1	746:784	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	8	22	dep	CONCLUSION	1027:1036	arg1	showed					1069:1074	showed	1069:1074	showed	1069:1074	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	1	23	theme	sweeteners	199:208	arg1	consumption					215:225	artificial sweeteners (AS) consumption	188:225	artificial sweeteners (AS) consumption	188:225	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	7	24	theme	ex vivo	938:944	arg1	function					963:970	ex vivo aortic vasomotor function	938:970	ex vivo aortic vasomotor function	938:970	Body fat composition has been evaluated and ex vivo aortic vasomotor function has been investigated with a pharmacological approach.					
32278610	0	25	theme	Artificial	0:9	arg1	sweeteners					11:20	Artificial sweeteners	0:20	Artificial sweeteners	0:20	Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents.					
32278610	3	26	theme	consumption	442:452	arg1	effect					421:426	the effect	417:426	the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease	417:538	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	3	27	theme	healthy	393:399	arg1	population					405:414	a healthy rat population	391:414	a healthy rat population	391:414	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	5	28	from	15	824:825	arg1	intake					814:819	high intake	809:819	high intake at 15 and 15 mg.kg-1	809:840	day-1, respectively or high intake at 15 and 15 mg.kg-1.					
32278610	5	28	from	15	824:825	arg1	day-1					786:790	day-1	786:790	day-1	786:790	day-1, respectively or high intake at 15 and 15 mg.kg-1.					
32278610	4	29	from	1	770:770	arg1	consumption					755:765	moderate consumption	746:765	moderate consumption at 1 and 2 mg.kg-1	746:784	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	4	30	theme	sucralose/acesulfame	673:692	arg1	solution					704:711	a sucralose/acesulfame potassium solution	671:711	a sucralose/acesulfame potassium solution at different concentrations	671:739	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	3	31	theme	rat	401:403	arg1	population					405:414	a healthy rat population	391:414	a healthy rat population	391:414	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	4	32	theme	Healthy	561:567	arg1	rats					576:579	Healthy Wistar rats	561:579	Healthy Wistar rats	561:579	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	8	33	theme	significant	1078:1088	arg1	increase					1090:1097	a significant increase	1076:1097	a significant increase in subcutaneous and perirenal adipose tissue mass storage	1076:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	3	34	theme	chronic	431:437	arg1	consumption					442:452	chronic AS consumption	431:452	chronic AS consumption	431:452	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	0	35	theme	endothelial	29:39	arg1	reactivity					50:59	endothelial vascular reactivity	29:59	endothelial vascular reactivity: Preliminary results in rodents	29:91	Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents.					
32278610	8	36	theme	subcutaneous	1102:1113	arg1	storage					1149:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	9	37	theme	Daily	1244:1248	arg1	Intake					1250:1255	Acceptable Daily Intake	1233:1255	Acceptable Daily Intake (ADI) concentration	1233:1275	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	9	37	theme	Daily	1244:1248	arg1	ADI					1258:1260	ADI	1258:1260	ADI	1258:1260	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	5	38	theme	high	809:812	arg1	intake					814:819	high intake	809:819	high intake at 15 and 15 mg.kg-1	809:840	day-1, respectively or high intake at 15 and 15 mg.kg-1.					
32278610	4	39	theme	water	643:647	arg1	sweetened					649:657	water sweetened	643:657	water sweetened	643:657	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	6	40	theme	daily	879:883	arg1	intake					885:890	acceptable daily intake	868:890	acceptable daily intake	868:890	day-1 for both molecules (acceptable daily intake).					
32278610	6	40	theme	daily	879:883	arg1	day-1					842:846	day-1	842:846	day-1 for both molecules (acceptable daily intake).	842:892	day-1 for both molecules (acceptable daily intake).					
32278610	1	41	theme	Prospective	114:124	arg1	studies					142:148	Prospective epidemiological studies	114:148	Prospective epidemiological studies	114:148	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	10	42	theme	observed	1434:1441	arg1	increase					1443:1450	the observed increase	1430:1450	the observed increase in cardiometabolic risk	1430:1474	These results are interesting because they will help to better explain the observed increase in cardiometabolic risk.					
32278610	4	43	theme	Wistar	569:574	arg1	rats					576:579	Healthy Wistar rats	561:579	Healthy Wistar rats	561:579	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	8	44	theme	perirenal	1119:1127	arg1	storage					1149:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	6	45	theme	acceptable	868:877	arg1	intake					885:890	acceptable daily intake	868:890	acceptable daily intake	868:890	day-1 for both molecules (acceptable daily intake).					
32278610	6	45	theme	acceptable	868:877	arg1	day-1					842:846	day-1	842:846	day-1 for both molecules (acceptable daily intake).	842:892	day-1 for both molecules (acceptable daily intake).					
32278610	0	46	theme	vascular	41:48	arg1	reactivity					50:59	endothelial vascular reactivity	29:59	endothelial vascular reactivity: Preliminary results in rodents	29:91	Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents.					
32278610	8	47	from	increase	1090:1097	arg1	storage					1149:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	9	48	theme	Intake	1250:1255	arg1	concentration					1263:1275	Acceptable Daily Intake (ADI) concentration	1233:1275	Acceptable Daily Intake (ADI) concentration	1233:1275	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	3	49	theme	body	457:460	arg1	marker					506:511	an early marker	497:511	an early marker for cardiovascular disease	497:538	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	3	49	theme	body	457:460	arg1	composition					462:472	body composition	457:472	body composition	457:472	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	0	50	theme	Preliminary	62:72	arg1	results					74:80	Preliminary results	62:80	endothelial vascular reactivity: Preliminary results in rodents	29:91	Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents.					
32278610	8	51	theme	tissue	1137:1142	arg1	storage					1149:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	0	52	from	results	74:80	arg1	rodents					85:91	rodents	85:91	rodents	85:91	Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents.					
32278610	1	53	dep	sweeteners	199:208	arg1	AS					211:212	AS	211:212	AS	211:212	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	1	54	theme	epidemiological	126:140	arg1	studies					142:148	Prospective epidemiological studies	114:148	Prospective epidemiological studies	114:148	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	9	55	theme	endothelial	1309:1319	arg1	dysfunction					1321:1331	a significant vascular endothelial dysfunction	1286:1331	a significant vascular endothelial dysfunction compared to other groups	1286:1356	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	7	56	theme	aortic	946:951	arg1	function					963:970	ex vivo aortic vasomotor function	938:970	ex vivo aortic vasomotor function	938:970	Body fat composition has been evaluated and ex vivo aortic vasomotor function has been investigated with a pharmacological approach.					
32278610	8	57	theme	adipose	1129:1135	arg1	storage					1149:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	4	58	with	diet	609:612	arg1	solution					704:711	a sucralose/acesulfame potassium solution	671:711	a sucralose/acesulfame potassium solution at different concentrations	671:739	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	4	59	theme	moderate	746:753	arg1	consumption					755:765	moderate consumption	746:765	moderate consumption at 1 and 2 mg.kg-1	746:784	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	3	60	theme	AS	439:440	arg1	consumption					442:452	chronic AS consumption	431:452	chronic AS consumption	431:452	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	2	61	theme	underlying	289:298	arg1	mechanisms					300:309	underlying mechanisms	289:309	underlying mechanisms	289:309	However, underlying mechanisms remain unknown.					
32278610	3	62	theme	vascular	478:485	arg1	function					487:494	vascular function	478:494	vascular function	478:494	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	8	63	theme	mass	1144:1147	arg1	storage					1149:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	subcutaneous and perirenal adipose tissue mass storage	1102:1155	CONCLUSION Both groups of AS-treated rats showed a significant increase in subcutaneous and perirenal adipose tissue mass storage, without changes in total body mass.					
32278610	9	64	theme	other	1345:1349	arg1	groups					1351:1356	other groups	1345:1356	other groups	1345:1356	However, rats that have consumed AS at Acceptable Daily Intake (ADI) concentration revealed a significant vascular endothelial dysfunction compared to other groups.					
32278610	4	65	theme	potassium	694:702	arg1	solution					704:711	a sucralose/acesulfame potassium solution	671:711	a sucralose/acesulfame potassium solution at different concentrations	671:739	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	7	66	theme	fat	899:901	arg1	composition					903:913	Body fat composition	894:913	Body fat composition	894:913	Body fat composition has been evaluated and ex vivo aortic vasomotor function has been investigated with a pharmacological approach.					
32278610	1	67	theme	developing	243:252	arg1	diseases					270:277	developing cardiometabolic diseases	243:277	developing cardiometabolic diseases	243:277	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	3	68	theme	preliminary	349:359	arg1	study					361:365	this preliminary study	344:365	this preliminary study	344:365	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	4	69	theme	sweetened	649:657	arg1	consumption					628:638	the consumption	624:638	the consumption of water sweetened	624:657	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	7	70	theme	pharmacological	1001:1015	arg1	approach					1017:1024	a pharmacological approach	999:1024	a pharmacological approach	999:1024	Body fat composition has been evaluated and ex vivo aortic vasomotor function has been investigated with a pharmacological approach.					
32278610	4	71	from	concentrations	726:739	arg1	solution					704:711	a sucralose/acesulfame potassium solution	671:711	a sucralose/acesulfame potassium solution at different concentrations	671:739	METHODS AND RESULTS Healthy Wistar rats followed a 10-week standard diet including the consumption of water sweetened or not with a sucralose/acesulfame potassium solution at different concentrations: for moderate consumption at 1 and 2 mg.kg-1.					
32278610	1	72	theme	cardiometabolic	254:268	arg1	diseases					270:277	developing cardiometabolic diseases	243:277	developing cardiometabolic diseases	243:277	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	3	73	theme	study	361:365	arg1	aim					337:339	the aim	333:339	the aim of this preliminary study	333:365	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	3	74	theme	cardiovascular	517:530	arg1	disease					532:538	cardiovascular disease	517:538	cardiovascular disease	517:538	Thus, the aim of this preliminary study was to characterize, in a healthy rat population, the effect of chronic AS consumption on body composition and vascular function, an early marker for cardiovascular disease.					
32278610	10	75	theme	cardiometabolic	1455:1469	arg1	risk					1471:1474	cardiometabolic risk	1455:1474	cardiometabolic risk	1455:1474	These results are interesting because they will help to better explain the observed increase in cardiometabolic risk.					
32278610	1	76	theme	diseases	270:277	arg1	consumption					215:225	artificial sweeteners (AS) consumption	188:225	artificial sweeteners (AS) consumption	188:225	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
32278610	1	76	theme	diseases	270:277	arg1	risk					235:238	the risk	231:238	the risk of developing cardiometabolic diseases	231:277	BACKGROUND AND AIMS Prospective epidemiological studies highlighted recently the link between artificial sweeteners (AS) consumption and the risk of developing cardiometabolic diseases.					
33620195	9	0	dep	wearing	1599:1605	arg1	feel					1607:1610	feel	1607:1610	feel	1607:1610	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	5	1	theme	external	1050:1057	arg1	stimulus					1059:1066	any external stimulus	1046:1066	any external stimulus	1046:1066	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	4	2	theme	borate	821:826	arg1	bonds					834:838	borate ester bonds	821:838	borate ester bonds	821:838	The composite hydrogel network was built by borate ester bonds and hydrogen bonds.					
33620195	7	3	theme	durable	1344:1350	arg1	stability					1352:1360	durable stability	1344:1360	durable stability	1344:1360	The hydrogel was further applied as a strain sensor for monitoring large and tiny human motions with durable stability.					
33620195	2	4	theme	hydrogel-based	336:349	arg1	devices					360:366	hydrogel-based wearable devices	336:366	hydrogel-based wearable devices	336:366	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	4	5	theme	hydrogen	844:851	arg1	bonds					853:857	hydrogen bonds	844:857	hydrogen bonds	844:857	The composite hydrogel network was built by borate ester bonds and hydrogen bonds.					
33620195	1	6	theme	electronic	253:262	arg1	skins					264:268	electronic skins	253:268	electronic skins	253:268	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	2	7	theme	mechanical	472:481	arg1	strength					483:490	good mechanical strength	467:490	good mechanical strength	467:490	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	8	8	theme	good	1410:1413	arg1	behavior					1423:1430	good sensing behavior	1410:1430	good sensing behavior	1410:1430	Significantly, the healed hydrogel also showed good sensing behavior.					
33620195	1	9	theme	Hydrogel-based	147:160	arg1	devices					171:177	Hydrogel-based wearable devices	147:177	Hydrogel-based wearable devices	147:177	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	0	10	theme	alcohol	90:96	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	9	11	theme	hydrogel	1565:1572	arg1	sensors					1574:1580	hydrogel sensors	1565:1580	hydrogel sensors	1565:1580	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	4	12	theme	composite	781:789	arg1	network					800:806	The composite hydrogel network	777:806	The composite hydrogel network	777:806	The composite hydrogel network was built by borate ester bonds and hydrogen bonds.					
33620195	5	13	theme	high	922:925	arg1	stretchability					927:940	high stretchability	922:940	high stretchability (780% strain)	922:954	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	5	13	theme	high	922:925	arg1	strain					948:953	780% strain	943:953	780% strain	943:953	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	0	14	theme	vinyl	84:88	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	4	15	theme	ester	828:832	arg1	bonds					834:838	borate ester bonds	821:838	borate ester bonds	821:838	The composite hydrogel network was built by borate ester bonds and hydrogen bonds.					
33620195	5	16	theme	%	946:946	arg1	stretchability					927:940	high stretchability	922:940	high stretchability (780% strain)	922:954	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	5	16	theme	%	946:946	arg1	strain					948:953	780% strain	943:953	780% strain	943:953	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	1	17	theme	soft	271:274	arg1	robotics					276:283	soft robotics	271:283	soft robotics	271:283	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	0	18	theme	Composite	99:107	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	8	19	theme	sensing	1415:1421	arg1	behavior					1423:1430	good sensing behavior	1410:1430	good sensing behavior	1410:1430	Significantly, the healed hydrogel also showed good sensing behavior.					
33620195	7	20	theme	strain	1281:1286	arg1	sensor					1288:1293	a strain sensor	1279:1293	a strain sensor for monitoring large and tiny human motions with durable stability	1279:1360	The hydrogel was further applied as a strain sensor for monitoring large and tiny human motions with durable stability.					
33620195	7	20	theme	strain	1281:1286	arg1	hydrogel					1247:1254	The hydrogel	1243:1254	The hydrogel	1243:1254	The hydrogel was further applied as a strain sensor for monitoring large and tiny human motions with durable stability.					
33620195	7	21	theme	large	1310:1314	arg1	motions					1331:1337	large and tiny human motions	1310:1337	large and tiny human motions	1310:1337	The hydrogel was further applied as a strain sensor for monitoring large and tiny human motions with durable stability.					
33620195	6	22	theme	considerable	1106:1117	arg1	behavior					1130:1137	considerable conductive behavior	1106:1137	considerable conductive behavior	1106:1137	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	1	23	theme	wearable	162:169	arg1	devices					171:177	Hydrogel-based wearable devices	147:177	Hydrogel-based wearable devices	147:177	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	6	24	dep	=	1213:1213	arg1	15.98					1215:1219	15.98	1215:1219	15.98	1215:1219	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	3	25	theme	sodium	703:708	arg1	alginate					710:717	sodium alginate	703:717	sodium alginate (SA)	703:722	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	3	25	theme	sodium	703:708	arg1	SA					720:721	SA	720:721	SA	720:721	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	2	26	theme	good	467:470	arg1	strength					483:490	good mechanical strength	467:490	good mechanical strength	467:490	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	7	27	theme	tiny	1320:1323	arg1	motions					1331:1337	large and tiny human motions	1310:1337	large and tiny human motions	1310:1337	The hydrogel was further applied as a strain sensor for monitoring large and tiny human motions with durable stability.					
33620195	0	28	theme	Multifunctional	2:16	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	0	29	theme	Alginate/Poly	70:82	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	2	30	theme	high	496:499	arg1	stretchability					501:514	high stretchability	496:514	high stretchability	496:514	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	3	31	theme	multifunctional	577:591	arg1	hydrogels					614:622	multifunctional conductive composite hydrogels	577:622	multifunctional conductive composite hydrogels	577:622	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	6	32	theme	%	1239:1239	arg1	strain					1226:1231	a strain	1224:1231	a strain of 780%	1224:1239	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	7	33	theme	human	1325:1329	arg1	motions					1331:1337	large and tiny human motions	1310:1337	large and tiny human motions	1310:1337	The hydrogel was further applied as a strain sensor for monitoring large and tiny human motions with durable stability.					
33620195	3	34	theme	conductive	593:602	arg1	hydrogels					614:622	multifunctional conductive composite hydrogels	577:622	multifunctional conductive composite hydrogels	577:622	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	9	35	with	application	1550:1560	arg1	sensitivity					1621:1631	high sensitivity	1616:1631	high sensitivity	1616:1631	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	9	35	with	application	1550:1560	arg1	wearing					1599:1605	comfortable wearing feel	1587:1610	comfortable wearing feel	1587:1610	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	0	36	theme	Self-Healing	19:30	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	3	37	theme	tannic	729:734	arg1	TA					742:743	TA	742:743	TA	742:743	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	3	37	theme	tannic	729:734	arg1	acid					736:739	tannic acid	729:739	tannic acid (TA)	729:744	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	6	38	theme	resistance	1161:1170	arg1	changes					1150:1156	stable changes	1143:1156	stable changes of resistance	1143:1170	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	6	38	theme	resistance	1161:1170	arg1	behavior					1130:1137	considerable conductive behavior	1106:1137	considerable conductive behavior	1106:1137	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	1	39	theme	tremendous	194:203	arg1	interest					205:212	tremendous interest	194:212	tremendous interest	194:212	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	2	40	theme	self-healing	411:422	arg1	ability					424:430	a self-healing ability	409:430	a self-healing ability	409:430	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	9	41	theme	high	1616:1619	arg1	sensitivity					1621:1631	high sensitivity	1616:1631	high sensitivity	1616:1631	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	9	42	theme	comfortable	1587:1597	arg1	wearing					1599:1605	comfortable wearing feel	1587:1610	comfortable wearing feel	1587:1610	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	5	43	theme	self-healing	966:977	arg1	performance					979:989	fast self-healing performance	961:989	fast self-healing performance	961:989	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	0	44	theme	Self-Adhesive	33:45	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	0	45	theme	Flexible	123:130	arg1	Sensor					139:144	a Flexible Strain Sensor	121:144	a Flexible Strain Sensor	121:144	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	6	46	dep	sensitivity	1182:1192	arg1	=					1213:1213	=	1213:1213	=	1213:1213	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	5	47	theme	fast	961:964	arg1	performance					979:989	fast self-healing performance	961:989	fast self-healing performance	961:989	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	2	48	theme	high	390:393	arg1	conductivity					395:406	high conductivity	390:406	high conductivity	390:406	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	1	49	from	applications	237:248	arg1	skins					264:268	electronic skins	253:268	electronic skins	253:268	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	1	49	from	applications	237:248	arg1	robotics					276:283	soft robotics	271:283	soft robotics	271:283	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	1	49	from	applications	237:248	arg1	sensors					290:296	sensors	290:296	sensors	290:296	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	5	50	theme	high	1023:1026	arg1	efficiency					1004:1013	healing efficiency	996:1013	healing efficiency (HE) as high as 93.56% without any external stimulus	996:1066	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	5	50	theme	high	1023:1026	arg1	HE					1016:1017	HE	1016:1017	HE	1016:1017	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	3	51	theme	composite	604:612	arg1	hydrogels					614:622	multifunctional conductive composite hydrogels	577:622	multifunctional conductive composite hydrogels	577:622	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	6	52	theme	conductive	1119:1128	arg1	behavior					1130:1137	considerable conductive behavior	1106:1137	considerable conductive behavior	1106:1137	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	0	53	theme	Strain	132:137	arg1	Sensor					139:144	a Flexible Strain Sensor	121:144	a Flexible Strain Sensor	121:144	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	4	54	theme	hydrogel	791:798	arg1	network					800:806	The composite hydrogel network	777:806	The composite hydrogel network	777:806	The composite hydrogel network was built by borate ester bonds and hydrogen bonds.					
33620195	9	55	theme	sensors	1574:1580	arg1	application					1550:1560	the application	1546:1560	the application of hydrogel sensors with comfortable wearing feel and high sensitivity	1546:1631	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	5	56	theme	obtained	864:871	arg1	hydrogel					873:880	The obtained hydrogel	860:880	The obtained hydrogel	860:880	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	6	57	from	strain	1226:1231	arg1	=					1213:1213	=	1213:1213	=	1213:1213	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	9	58	theme	biocompatible	1497:1509	arg1	hydrogels					1525:1533	biocompatible polymer-based hydrogels	1497:1533	biocompatible polymer-based hydrogels	1497:1533	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	5	59	theme	healing	996:1002	arg1	efficiency					1004:1013	healing efficiency	996:1013	healing efficiency (HE) as high as 93.56% without any external stimulus	996:1066	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	5	59	theme	healing	996:1002	arg1	HE					1016:1017	HE	1016:1017	HE	1016:1017	The obtained hydrogel exhibited pH- and sugar-responsiveness, high stretchability (780% strain), and fast self-healing performance with healing efficiency (HE) as high as 93.56% without any external stimulus.					
33620195	3	60	theme	simple	645:650	arg1	method					660:665	a simple one-pot method	643:665	a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker	643:774	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	6	61	theme	high	1177:1180	arg1	sensitivity					1182:1192	high sensitivity	1177:1192	high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%)	1177:1240	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	8	62	theme	healed	1382:1387	arg1	hydrogel					1389:1396	the healed hydrogel	1378:1396	the healed hydrogel	1378:1396	Significantly, the healed hydrogel also showed good sensing behavior.					
33620195	3	63	theme	one-pot	652:658	arg1	method					660:665	a simple one-pot method	643:665	a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker	643:774	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	1	64	theme	potential	227:235	arg1	applications					237:248	their potential applications	221:248	their potential applications in electronic skins, soft robotics, and sensors	221:296	Hydrogel-based wearable devices have attracted tremendous interest due to their potential applications in electronic skins, soft robotics, and sensors.					
33620195	0	65	theme	Sodium	63:68	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	3	66	theme	vinyl	681:685	arg1	poly					676:679	poly	676:679	poly(vinyl alcohol) (PVA)	676:700	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	3	66	theme	vinyl	681:685	arg1	alcohol					687:693	vinyl alcohol	681:693	vinyl alcohol	681:693	Herein, multifunctional conductive composite hydrogels were fabricated by a simple one-pot method based on poly(vinyl alcohol) (PVA), sodium alginate (SA), and tannic acid (TA) using borax as a cross-linker.					
33620195	6	67	theme	stable	1143:1148	arg1	changes					1150:1156	stable changes	1143:1156	stable changes of resistance	1143:1170	Additionally, the hydrogel displayed considerable conductive behavior and stable changes of resistance with high sensitivity (gauge factor (GF) = 15.98 at a strain of 780%).					
33620195	9	68	theme	hydrogels	1525:1533	arg1	preparation					1482:1492	preparation	1482:1492	preparation	1482:1492	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	9	68	theme	hydrogels	1525:1533	arg1	design					1471:1476	design	1471:1476	design	1471:1476	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	9	69	dep	design	1471:1476	arg1	the					1467:1469	the	1467:1469	the	1467:1469	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	0	70	theme	Conductive	52:61	arg1	Hydrogel					109:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel	0:116	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.	0:145	A Multifunctional, Self-Healing, Self-Adhesive, and Conductive Sodium Alginate/Poly(vinyl alcohol) Composite Hydrogel as a Flexible Strain Sensor.					
33620195	2	71	theme	wearable	351:358	arg1	devices					360:366	hydrogel-based wearable devices	336:366	hydrogel-based wearable devices	336:366	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
33620195	9	72	theme	polymer-based	1511:1523	arg1	hydrogels					1525:1533	biocompatible polymer-based hydrogels	1497:1533	biocompatible polymer-based hydrogels	1497:1533	This work broadens the avenue for the design and preparation of biocompatible polymer-based hydrogels to promote the application of hydrogel sensors with comfortable wearing feel and high sensitivity.					
33620195	2	73	theme	good	517:520	arg1	biocompatibility					522:537	good biocompatibility	517:537	good biocompatibility	517:537	However, it is still a challenge for hydrogel-based wearable devices to be integrated with high conductivity, a self-healing ability, remoldability, self-adhesiveness, good mechanical strength and high stretchability, good biocompatibility, and stimulus-responsiveness.					
32204068	7	0	theme	first-order	1240:1250	arg1	model					1260:1264	first-order kinetic model	1240:1264	first-order kinetic model	1240:1264	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	9	1	theme	cytotoxicity	1554:1565	arg1	test					1567:1570	MTT cytotoxicity test	1550:1570	MTT cytotoxicity test	1550:1570	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	1	2	theme	NIPA-co-MBA	359:369	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	2	theme	NIPA-co-MBA	359:369	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	0	3	theme	pH/temperature-responsive	112:136	arg1	nanocomposites					138:151	pH/temperature-responsive nanocomposites	112:151	pH/temperature-responsive nanocomposites	112:151	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers.					
32204068	1	4	theme	-delivery	422:430	arg1	devices					432:438	erlotinib (ERL)-delivery devices	407:438	erlotinib (ERL)-delivery devices for lung cancer cells	407:460	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	9	5	theme	pristine	1494:1501	arg1	drug					1503:1506	the pristine drug	1490:1506	the pristine drug	1490:1506	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	6	6	theme	initial	1141:1147	arg1	release					1155:1161	an initial burst release	1138:1161	an initial burst release of drug	1138:1169	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	3	7	theme	NCs	725:727	arg1	crosslinks					705:714	the crosslinks	701:714	the crosslinks of these NCs	701:727	The molar mass (M¯c) between the crosslinks of these NCs was declined with temperature.					
32204068	2	8	theme	BEN	650:652	arg1	contents					654:661	their BEN contents	644:661	their BEN contents	644:661	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	1	9	dep	nanocomposites	383:396	arg1	N'-methylene-bis-acrylamide					311:337	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	N'-methylene-bis-acrylamide	311:337	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	6	10	dep	profiles	1110:1117	arg1	Q8h					1120:1122	Q8h	1120:1122	Q8h	1120:1122	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	6	10	dep	profiles	1110:1117	arg1	%					1130:1130	62-98%	1125:1130	62-98%	1125:1130	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	1	11	theme	-BEN	371:374	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	11	theme	-BEN	371:374	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	5	12	theme	ERL-loaded	879:888	arg1	F-1-F-3					900:906	F-1-F-3	900:906	F-1-F-3	900:906	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	5	12	theme	ERL-loaded	879:888	arg1	matrices					890:897	The corresponding ERL-loaded matrices	861:897	The corresponding ERL-loaded matrices (F-1-F-3)	861:907	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	9	13	theme	AO/EB	1576:1580	arg1	assay					1591:1595	AO/EB staining assay	1576:1595	AO/EB staining assay	1576:1595	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	8	14	theme	Freudlich	1359:1367	arg1	isotherms					1369:1377	Freudlich isotherms	1359:1377	Freudlich isotherms	1359:1377	The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms.					
32204068	4	15	dep	XRD	823:825	arg1	analyses					851:858	analyses	851:858	analyses	851:858	The composite architecture of these scaffolds was confirmed by XRD, FTIR, TGA, DSC and SEM analyses.					
32204068	1	16	theme	lung	444:447	arg1	cells					456:460	lung cancer cells	444:460	lung cancer cells	444:460	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	2	17	dep	exhibited	518:526	arg1	%					668:668	0-20%	664:668	0-20%	664:668	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	5	18	theme	corresponding	865:877	arg1	F-1-F-3					900:906	F-1-F-3	900:906	F-1-F-3	900:906	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	5	18	theme	corresponding	865:877	arg1	matrices					890:897	The corresponding ERL-loaded matrices	861:897	The corresponding ERL-loaded matrices (F-1-F-3)	861:907	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	9	19	theme	staining	1582:1589	arg1	assay					1591:1595	AO/EB staining assay	1576:1595	AO/EB staining assay	1576:1595	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	5	20	theme	drug	931:934	arg1	efficiency					950:959	outstanding drug encapsulation efficiency	919:959	outstanding drug encapsulation efficiency (DEE, 93-100%)	919:974	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	9	21	theme	greater	1470:1476	arg1	extent					1478:1483	a greater extent	1468:1483	a greater extent than the pristine drug	1468:1506	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	1	22	theme	cancer	449:454	arg1	cells					456:460	lung cancer cells	444:460	lung cancer cells	444:460	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	2	23	theme	outstanding	528:538	arg1	biodegradability					540:555	outstanding biodegradability	528:555	outstanding biodegradability	528:555	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	1	24	theme	carboxymethyl	238:250	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	24	theme	carboxymethyl	238:250	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	0	25	theme	fenugreek	14:22	arg1	-clay					100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay	0:104	-clay	100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers.					
32204068	7	26	theme	kinetic	1252:1258	arg1	model					1260:1264	first-order kinetic model	1240:1264	first-order kinetic model	1240:1264	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	7	27	theme	optimized	1208:1216	arg1	F-3					1228:1230	F-3	1228:1230	F-3	1228:1230	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	7	27	theme	optimized	1208:1216	arg1	matrices					1218:1225	the optimized matrices	1204:1225	the optimized matrices (F-3)	1204:1231	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	1	28	theme	fenugreek	252:260	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	28	theme	fenugreek	252:260	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	0	29	theme	Carboxymethyl	0:12	arg1	-clay					100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay	0:104	-clay	100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers.					
32204068	1	30	theme	based	377:381	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	30	theme	based	377:381	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	3	31	theme	molar	676:680	arg1	M¯c					688:690	M¯c	688:690	M¯c	688:690	The molar mass (M¯c) between the crosslinks of these NCs was declined with temperature.					
32204068	3	31	theme	molar	676:680	arg1	mass					682:685	The molar mass	672:685	The molar mass (M¯c) between the crosslinks of these NCs	672:727	The molar mass (M¯c) between the crosslinks of these NCs was declined with temperature.					
32204068	1	32	theme	galactomannang-g-poly	262:282	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	32	theme	galactomannang-g-poly	262:282	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	33	theme	nanocomposites	383:396	arg1	synthesis					204:212	the synthesis	200:212	the synthesis	200:212	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	33	theme	nanocomposites	383:396	arg1	characterization					218:233	characterization	218:233	characterization	218:233	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	9	34	theme	A549	1418:1421	arg1	proliferation					1428:1440	A549 cell proliferation	1418:1440	A549 cell proliferation	1418:1440	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	2	35	theme	blank	508:512	arg1	NCs					514:516	The blank NCs	504:516	The blank NCs	504:516	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	0	36	theme	galactomannan-g-poly	24:43	arg1	-clay					100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay	0:104	-clay	100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers.					
32204068	9	37	theme	uptake	1533:1538	arg1	analysis					1540:1547	cellular uptake analysis	1524:1547	cellular uptake analysis	1524:1547	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	0	38	dep	-clay	100:104	arg1	N'-methylene-bis-acrylamide					72:98	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay	0:104	N'-methylene-bis-acrylamide	72:98	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers.					
32204068	1	39	theme	N-isopropylacrylamide-co-N	284:309	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	39	theme	N-isopropylacrylamide-co-N	284:309	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	7	40	theme	dissolution	1181:1191	arg1	pattern					1193:1199	The drug dissolution pattern	1172:1199	The drug dissolution pattern of the optimized matrices (F-3)	1172:1231	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	6	41	theme	drug	1166:1169	arg1	release					1155:1161	an initial burst release	1138:1161	an initial burst release of drug	1138:1169	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	9	42	theme	cellular	1524:1531	arg1	uptake					1533:1538	cellular uptake	1524:1538	cellular uptake analysis	1524:1547	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	7	43	theme	matrices	1218:1225	arg1	pattern					1193:1199	The drug dissolution pattern	1172:1199	The drug dissolution pattern of the optimized matrices (F-3)	1172:1231	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	5	44	dep	efficiency	950:959	arg1	DEE					962:964	DEE	962:964	DEE	962:964	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	5	44	dep	efficiency	950:959	arg1	%					973:973	93-100%	967:973	93-100%	967:973	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	8	45	theme	F-3	1336:1338	arg1	behavior					1324:1331	The mucin adsorption behavior	1303:1331	The mucin adsorption behavior of F-3	1303:1338	The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms.					
32204068	8	46	theme	mucin	1307:1311	arg1	behavior					1324:1331	The mucin adsorption behavior	1303:1331	The mucin adsorption behavior of F-3	1303:1338	The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms.					
32204068	0	47	theme	N-isopropylacrylamide-co-N	45:70	arg1	-clay					100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay	0:104	-clay	100:104	Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers.					
32204068	9	48	theme	ERL-loaded	1384:1393	arg1	formulation					1395:1405	The ERL-loaded formulation	1380:1405	The ERL-loaded formulation	1380:1405	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	2	49	theme	swelling	586:593	arg1	profiles					595:602	outstanding biodegradability and pH/temperature-dependent swelling profiles	528:602	outstanding biodegradability and pH/temperature-dependent swelling profiles	528:602	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	1	50	theme	excessive	474:482	arg1	proliferation					489:501	excessive cell proliferation	474:501	excessive cell proliferation	474:501	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	5	51	theme	outstanding	919:929	arg1	efficiency					950:959	outstanding drug encapsulation efficiency	919:959	outstanding drug encapsulation efficiency (DEE, 93-100%)	919:974	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	2	52	theme	pH/temperature-dependent	561:584	arg1	swelling					586:593	pH/temperature-dependent swelling	561:593	pH/temperature-dependent swelling	561:593	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	9	53	theme	MTT	1550:1552	arg1	test					1567:1570	MTT cytotoxicity test	1550:1570	MTT cytotoxicity test	1550:1570	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	1	54	theme	cell	484:487	arg1	proliferation					489:501	excessive cell proliferation	474:501	excessive cell proliferation	474:501	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	55	theme	current	175:181	arg1	study					183:187	The current study	171:187	The current study	171:187	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	7	56	theme	drug	1176:1179	arg1	pattern					1193:1199	The drug dissolution pattern	1172:1199	The drug dissolution pattern of the optimized matrices (F-3)	1172:1231	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	4	57	theme	scaffolds	796:804	arg1	architecture					774:785	The composite architecture	760:785	The composite architecture of these scaffolds	760:804	The composite architecture of these scaffolds was confirmed by XRD, FTIR, TGA, DSC and SEM analyses.					
32204068	7	58	theme	Fickian	1284:1290	arg1	diffusion					1292:1300	Fickian diffusion	1284:1300	Fickian diffusion	1284:1300	The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion.					
32204068	6	59	theme	sustained	1088:1096	arg1	profiles					1110:1117	sustained ERL elution profiles	1088:1117	sustained ERL elution profiles (Q8h, 62-98%)	1088:1131	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	5	60	theme	zeta	981:984	arg1	potential					986:994	zeta potential	981:994	zeta potential	981:994	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	9	61	theme	cell	1423:1426	arg1	proliferation					1428:1440	A549 cell proliferation	1418:1440	A549 cell proliferation	1418:1440	The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.					
32204068	6	62	theme	ERL	1098:1100	arg1	profiles					1110:1117	sustained ERL elution profiles	1088:1117	sustained ERL elution profiles (Q8h, 62-98%)	1088:1131	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	4	63	theme	composite	764:772	arg1	architecture					774:785	The composite architecture	760:785	The composite architecture of these scaffolds	760:804	The composite architecture of these scaffolds was confirmed by XRD, FTIR, TGA, DSC and SEM analyses.					
32204068	1	64	theme	-bentonite	339:348	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	64	theme	-bentonite	339:348	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	6	65	theme	elution	1102:1108	arg1	profiles					1110:1117	sustained ERL elution profiles	1088:1117	sustained ERL elution profiles (Q8h, 62-98%)	1088:1131	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	6	66	theme	burst	1149:1153	arg1	release					1155:1161	an initial burst release	1138:1161	an initial burst release of drug	1138:1169	These formulations demonstrated sustained ERL elution profiles (Q8h, 62-98%) with an initial burst release of drug.					
32204068	5	67	theme	encapsulation	936:948	arg1	efficiency					950:959	outstanding drug encapsulation efficiency	919:959	outstanding drug encapsulation efficiency (DEE, 93-100%)	919:974	The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93-100%) with zeta potential between -8 and -16 mV and diameter between 615 and 1258 nm.					
32204068	1	68	theme	[CFG-g-P	350:357	arg1	NCs					399:401	NCs	399:401	NCs	399:401	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	1	68	theme	[CFG-g-P	350:357	arg1	nanocomposites					383:396	carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites	238:396	nanocomposites	383:396	The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N'-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation.					
32204068	2	69	theme	biodegradability	540:555	arg1	profiles					595:602	outstanding biodegradability and pH/temperature-dependent swelling profiles	528:602	outstanding biodegradability and pH/temperature-dependent swelling profiles	528:602	The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0-20%).					
32204068	8	70	theme	adsorption	1313:1322	arg1	behavior					1324:1331	The mucin adsorption behavior	1303:1331	The mucin adsorption behavior of F-3	1303:1338	The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms.					
32421483	4	0	theme	cellular	835:842	arg1	response					844:851	cellular response	835:851	cellular response	835:851	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	2	1	theme	alcohol	336:342	arg1	blend					367:371	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	1	2	theme	excisional	243:252	arg1	wounds					254:259	excisional wounds	243:259	excisional wounds	243:259	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	7	3	theme	PVA/chitosan	1421:1432	arg1	scaffolds					1434:1442	sterile gauze or PVA/chitosan scaffolds	1404:1442	sterile gauze or PVA/chitosan scaffolds	1404:1442	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	5	4	theme	excisional	903:912	arg1	wounds					914:919	full-thickness excisional wounds	888:919	full-thickness excisional wounds	888:919	Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings.					
32421483	7	5	theme	loose	1459:1463	arg1	fibres					1474:1479	random and loose collagen fibres	1448:1479	random and loose collagen fibres	1448:1479	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	7	6	theme	gauze	1412:1416	arg1	scaffolds					1434:1442	sterile gauze or PVA/chitosan scaffolds	1404:1442	sterile gauze or PVA/chitosan scaffolds	1404:1442	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	3	7	theme	PVA/chitosan	481:492	arg1	blend					502:506	PVA/chitosan polymer blend	481:506	PVA/chitosan polymer blend	481:506	To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method.					
32421483	1	8	theme	fabricated	165:174	arg1	dressing					176:183	a fabricated dressing	163:183	a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds	163:259	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	7	9	theme	random	1448:1453	arg1	fibres					1474:1479	random and loose collagen fibres	1448:1479	random and loose collagen fibres	1448:1479	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	6	10	theme	wound	1226:1230	arg1	closure					1232:1238	wound closure	1226:1238	wound closure	1226:1238	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	3	11	theme	%	466:466	arg1	ratio					456:460	weight ratio	449:460	weight ratio of 5%	449:466	To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method.					
32421483	3	12	with	kaolin	437:442	arg1	ratio					456:460	weight ratio	449:460	weight ratio of 5%	449:466	To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method.					
32421483	3	13	theme	electrospinning	558:572	arg1	method					574:579	the electrospinning method	554:579	the electrospinning method	554:579	To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method.					
32421483	4	14	theme	L929	862:865	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	15	theme	vapour	686:691	arg1	rate					706:709	water vapour transmission rate	680:709	water vapour transmission rate	680:709	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	7	16	contain	had	1444:1446	arg1	wounds					1384:1389	wounds	1384:1389	wounds treated with sterile gauze or PVA/chitosan scaffolds	1384:1442	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	7	16	contain	had	1444:1446	arg2	fibres					1474:1479	random and loose collagen fibres	1448:1479	random and loose collagen fibres	1448:1479	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	7	17	theme	collagen	1349:1356	arg1	fibres					1358:1363	dense and regular collagen fibres	1331:1363	dense and regular collagen fibres	1331:1363	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	3	18	theme	polymer	494:500	arg1	blend					502:506	PVA/chitosan polymer blend	481:506	PVA/chitosan polymer blend	481:506	To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method.					
32421483	5	19	theme	full-thickness	888:901	arg1	wounds					914:919	full-thickness excisional wounds	888:919	full-thickness excisional wounds	888:919	Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings.					
32421483	4	20	theme	surface	659:665	arg1	wettability					667:677	surface wettability	659:677	surface wettability	659:677	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	21	with	test	801:804	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	0	22	theme	dressing	73:80	arg1	material					82:89	a wound dressing material	65:89	a wound dressing material	65:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	0	23	dep	studies	113:119	arg1	scaffold					53:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold	0:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material	0:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	1	24	contain	containing	185:194	arg1	dressing					176:183	a fabricated dressing	163:183	a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds	163:259	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	1	24	contain	containing	185:194	arg2	kaolin					196:201	kaolin	196:201	kaolin for skin regeneration	196:223	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	6	25	dep	RESULTS	984:990	arg1	showed					1002:1007	showed	1002:1007	showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively	1002:1252	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	4	26	theme	uptake	741:746	arg1	capacity					748:755	blood uptake capacity	735:755	blood uptake capacity	735:755	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	27	with	compatibility	764:776	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	0	28	dep	in	105:106	arg1	vivo					108:111	vivo	108:111	vivo	108:111	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	8	29	theme	potential	1514:1522	arg1	applicability					1524:1536	the potential applicability	1510:1536	the potential applicability of PVA/chitosan/kaolin scaffolds	1510:1569	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	8	29	theme	potential	1514:1522	arg1	material					1587:1594	a wound care material	1574:1594	a wound care material	1574:1594	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	0	30	theme	in	105:106	arg1	studies					113:119	in vitro and in vivo studies	92:119	in vitro and in vivo studies	92:119	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	5	31	theme	kaolin-free	961:971	arg1	dressings					973:981	kaolin-containing and kaolin-free dressings	939:981	kaolin-containing and kaolin-free dressings	939:981	Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings.					
32421483	7	32	theme	dense	1331:1335	arg1	fibres					1358:1363	dense and regular collagen fibres	1331:1363	dense and regular collagen fibres	1331:1363	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	4	33	theme	water-uptake	712:723	arg1	capacity					725:732	water-uptake capacity	712:732	water-uptake capacity	712:732	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	2	34	theme	polymer	359:365	arg1	blend					367:371	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	0	35	theme	Kaolin-loaded	0:12	arg1	scaffold					53:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold	0:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material	0:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	8	36	dep	CONCLUSION	1482:1491	arg1	show					1505:1508	show	1505:1508	show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material	1505:1594	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	8	37	theme	scaffolds	1561:1569	arg1	applicability					1524:1536	the potential applicability	1510:1536	the potential applicability of PVA/chitosan/kaolin scaffolds	1510:1569	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	8	37	theme	scaffolds	1561:1569	arg1	material					1587:1594	a wound care material	1574:1594	a wound care material	1574:1594	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	2	38	theme	nanofibrous	396:406	arg1	dressing					408:415	a composite nanofibrous dressing	384:415	a composite nanofibrous dressing	384:415	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	0	39	theme	alcohol	33:39	arg1	scaffold					53:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold	0:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material	0:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	6	40	theme	sterile	1167:1173	arg1	gauze					1175:1179	the sterile gauze	1163:1179	the sterile gauze	1163:1179	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	8	41	theme	care	1582:1585	arg1	material					1587:1594	a wound care material	1574:1594	a wound care material	1574:1594	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	8	41	theme	care	1582:1585	arg1	applicability					1524:1536	the potential applicability	1510:1536	the potential applicability of PVA/chitosan/kaolin scaffolds	1510:1569	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	4	42	with	microstructure	620:633	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	2	43	theme	polyvinyl	326:334	arg1	PVA					345:347	PVA	345:347	PVA	345:347	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	2	43	theme	polyvinyl	326:334	arg1	alcohol					336:342	electrospun polyvinyl alcohol	314:342	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	4	44	theme	microbial	779:787	arg1	test					801:804	microbial penetration test	779:804	microbial penetration test	779:804	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	1	45	theme	rat	230:232	arg1	model					234:238	a rat model	228:238	a rat model of excisional wounds	228:259	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	7	46	theme	collagen	1465:1472	arg1	fibres					1474:1479	random and loose collagen fibres	1448:1479	random and loose collagen fibres	1448:1479	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	2	47	theme	present	276:282	arg1	study					284:288	the present study	272:288	the present study	272:288	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	1	48	theme	wounds	254:259	arg1	model					234:238	a rat model	228:238	a rat model of excisional wounds	228:259	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	4	49	with	wettability	667:677	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	6	50	theme	closure	1232:1238	arg1	%					1205:1205	86.15±8.11%	1195:1205	86.15±8.11%	1195:1205	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	6	50	theme	closure	1232:1238	arg1	%					1221:1221	78.50±4.22%	1211:1221	78.50±4.22% of wound closure	1211:1238	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	6	50	theme	closure	1232:1238	arg1	closure					1232:1238	wound closure	1226:1238	wound closure	1226:1238	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	1	51	theme	dressing	176:183	arg1	application					148:158	the application	144:158	the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds	144:259	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	4	52	theme	colonies	821:828	arg1	number					811:816	the number	807:816	the number of colonies	807:828	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	rate					706:709	water vapour transmission rate	680:709	water vapour transmission rate	680:709	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	capacity					748:755	blood uptake capacity	735:755	blood uptake capacity	735:755	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	wettability					667:677	surface wettability	659:677	surface wettability	659:677	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	test					801:804	microbial penetration test	779:804	microbial penetration test	779:804	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	microstructure					620:633	their microstructure	614:633	their microstructure	614:633	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	capacity					725:732	water-uptake capacity	712:732	water-uptake capacity	712:732	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	response					844:851	cellular response	835:851	cellular response	835:851	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	compatibility					764:776	blood compatibility	758:776	blood compatibility	758:776	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	52	theme	colonies	821:828	arg1	properties					647:656	mechanical properties	636:656	mechanical properties	636:656	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	7	53	theme	sterile	1404:1410	arg1	scaffolds					1434:1442	sterile gauze or PVA/chitosan scaffolds	1404:1442	sterile gauze or PVA/chitosan scaffolds	1404:1442	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	4	54	theme	mechanical	636:645	arg1	properties					647:656	mechanical properties	636:656	mechanical properties	636:656	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	55	with	capacity	748:755	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	1	56	theme	skin	207:210	arg1	regeneration					212:223	skin regeneration	207:223	skin regeneration	207:223	OBJECTIVE To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds.					
32421483	4	57	with	capacity	725:732	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	58	theme	water	680:684	arg1	rate					706:709	water vapour transmission rate	680:709	water vapour transmission rate	680:709	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	59	with	response	844:851	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	60	theme	cell	867:870	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	4	61	theme	transmission	693:704	arg1	rate					706:709	water vapour transmission rate	680:709	water vapour transmission rate	680:709	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	7	62	theme	regular	1341:1347	arg1	fibres					1358:1363	dense and regular collagen fibres	1331:1363	dense and regular collagen fibres	1331:1363	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	2	63	dep	METHOD	262:267	arg1	loaded					302:307	loaded	302:307	was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing	298:415	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	0	64	theme	in	92:93	arg1	studies					113:119	in vitro and in vivo studies	92:119	in vitro and in vivo studies	92:119	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	4	65	with	number	811:816	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	5	66	theme	kaolin-containing	939:955	arg1	dressings					973:981	kaolin-containing and kaolin-free dressings	939:981	kaolin-containing and kaolin-free dressings	939:981	Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings.					
32421483	4	67	theme	blood	735:739	arg1	capacity					748:755	blood uptake capacity	735:755	blood uptake capacity	735:755	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	6	68	theme	significant	1076:1086	arg1	closure					1088:1094	a significant closure	1074:1094	a significant closure to nearly 97.62±4.81%	1074:1116	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	8	69	theme	PVA/chitosan/kaolin	1541:1559	arg1	scaffolds					1561:1569	PVA/chitosan/kaolin scaffolds	1541:1569	PVA/chitosan/kaolin scaffolds	1541:1569	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	0	70	theme	chitosan/polyvinyl	14:31	arg1	scaffold					53:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold	0:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material	0:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	0	71	dep	in	92:93	arg1	vitro					95:99	vitro	95:99	vitro	95:99	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	7	72	theme	PVA/chitosan/kaolin	1304:1322	arg1	group					1324:1328	the PVA/chitosan/kaolin group	1300:1328	the PVA/chitosan/kaolin group	1300:1328	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	5	73	with	Rats	878:881	arg1	wounds					914:919	full-thickness excisional wounds	888:919	full-thickness excisional wounds	888:919	Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings.					
32421483	2	74	theme	/chitosan	349:357	arg1	blend					367:371	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	0	75	theme	electrospun	41:51	arg1	scaffold					53:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold	0:60	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material	0:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	6	76	theme	kaolin-incorporated	1036:1054	arg1	mats					1056:1059	the kaolin-incorporated mats	1032:1059	the kaolin-incorporated mats	1032:1059	RESULTS The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively.					
32421483	8	77	theme	wound	1576:1580	arg1	material					1587:1594	a wound care material	1574:1594	a wound care material	1574:1594	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	8	77	theme	wound	1576:1580	arg1	applicability					1524:1536	the potential applicability	1510:1536	the potential applicability of PVA/chitosan/kaolin scaffolds	1510:1569	CONCLUSION Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.					
32421483	4	78	with	properties	647:656	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	2	79	theme	composite	386:394	arg1	dressing					408:415	a composite nanofibrous dressing	384:415	a composite nanofibrous dressing	384:415	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	4	80	theme	penetration	789:799	arg1	test					801:804	microbial penetration test	779:804	microbial penetration test	779:804	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	0	81	theme	wound	67:71	arg1	material					82:89	a wound dressing material	65:89	a wound dressing material	65:89	Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: in vitro and in vivo studies.					
32421483	7	82	theme	histopathological	1259:1275	arg1	studies					1277:1283	The histopathological studies	1255:1283	The histopathological studies	1255:1283	The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres.					
32421483	2	83	theme	electrospun	314:324	arg1	PVA					345:347	PVA	345:347	PVA	345:347	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	2	83	theme	electrospun	314:324	arg1	alcohol					336:342	electrospun polyvinyl alcohol	314:342	electrospun polyvinyl alcohol (PVA)/chitosan polymer blend	314:371	METHOD In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing.					
32421483	4	84	theme	blood	758:762	arg1	compatibility					764:776	blood compatibility	758:776	blood compatibility	758:776	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32421483	3	85	theme	weight	449:454	arg1	ratio					456:460	weight ratio	449:460	weight ratio of 5%	449:466	To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method.					
32421483	4	86	with	rate	706:709	arg1	line					872:875	the L929 cell line	858:875	the L929 cell line	858:875	Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line.					
32519374	2	0	used	used	185:188	arg2	CHI					151:153	CHI	151:153	CHI	151:153	Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation.					
32519374	2	0	used	used	185:188	arg2	Chitosan					141:148	Chitosan	141:148	Chitosan (CHI)	141:154	Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation.					
32519374	2	0	used	used	185:188	arg2	protein					165:171	whey protein	160:171	whey protein	160:171	Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation.					
32519374	7	1	theme	elongation	1113:1122	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	4	2	theme	mechanical	532:541	arg1	properties					543:552	mechanical properties	532:552	mechanical properties	532:552	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	0	3	theme	film	83:86	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	0	3	theme	film	83:86	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	4	4	theme	composite	468:476	arg1	film					478:481	a new composite film	462:481	a new composite film with strong water retention, bacteriostatic, and mechanical properties	462:552	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	5	5	theme	film	597:600	arg1	WVP					585:587	WVP	585:587	WVP	585:587	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	5	5	theme	film	597:600	arg1	permeability					571:582	The water vapor permeability	555:582	The water vapor permeability (WVP) of the film	555:600	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	9	6	theme	storage	1386:1392	arg1	days					1378:1381	16 days	1375:1381	16 days of storage of chestnuts at 25 °C	1375:1414	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	4	7	theme	new	464:466	arg1	film					478:481	a new composite film	462:481	a new composite film with strong water retention, bacteriostatic, and mechanical properties	462:552	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	5	8	theme	water	559:563	arg1	WVP					585:587	WVP	585:587	WVP	585:587	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	5	8	theme	water	559:563	arg1	permeability					571:582	The water vapor permeability	555:582	The water vapor permeability (WVP) of the film	555:600	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	7	9	theme	tensile	1091:1097	arg1	strength					1099:1106	tensile strength	1091:1106	tensile strength	1091:1106	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	3	10	theme	chestnut	339:346	arg1	preservation					348:359	chestnut preservation	339:359	chestnut preservation	339:359	However, the composite film composed of the two components does not yield satisfactory properties for chestnut preservation.					
32519374	4	11	theme	whey	439:442	arg1	protein					444:450	whey protein	439:450	whey protein	439:450	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	5	12	dep	%	705:705	arg1	w/v					708:710	w/v	708:710	w/v	708:710	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	5	13	theme	vapor	565:569	arg1	WVP					585:587	WVP	585:587	WVP	585:587	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	5	13	theme	vapor	565:569	arg1	permeability					571:582	The water vapor permeability	555:582	The water vapor permeability (WVP) of the film	555:600	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	7	14	theme	strength	1099:1106	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	8	15	theme	fungus	1265:1270	arg1	structure					1248:1256	the mycelial structure	1235:1256	the mycelial structure of the fungus	1235:1270	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	3	16	theme	satisfactory	311:322	arg1	properties					324:333	satisfactory properties	311:333	satisfactory properties for chestnut preservation	311:359	However, the composite film composed of the two components does not yield satisfactory properties for chestnut preservation.					
32519374	6	17	dep	%	850:850	arg1	w/v					853:855	w/v	853:855	w/v	853:855	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
32519374	7	18	theme	WS	1087:1088	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	19	theme	testing	1025:1031	arg1	34					1036:1037	34	1036:1037	34	1036:1037	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	19	theme	testing	1025:1031	arg1	L9					1033:1034	orthogonal testing L9	1014:1034	orthogonal testing L9(34 )	1014:1039	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	0	20	theme	Castanea	102:109	arg1	Bl					122:123	Castanea mollissima Bl.	102:124	Castanea mollissima Bl.	102:124	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	0	20	theme	Castanea	102:109	arg1	chestnut					92:99	chestnut	92:99	chestnut (Castanea mollissima Bl.)	92:125	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	9	21	from	25 °C	1410:1414	arg1	days					1378:1381	16 days	1375:1381	16 days of storage of chestnuts at 25 °C	1375:1414	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	9	21	from	25 °C	1410:1414	arg1	storage					1386:1392	storage	1386:1392	storage of chestnuts at 25 °C	1386:1414	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	6	22	theme	0.3	847:849	arg1	%					850:850	%	850:850	%	850:850	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
32519374	0	23	theme	mollissima	111:120	arg1	Bl					122:123	Castanea mollissima Bl.	102:124	Castanea mollissima Bl.	102:124	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	0	23	theme	mollissima	111:120	arg1	chestnut					92:99	chestnut	92:99	chestnut (Castanea mollissima Bl.)	92:125	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	5	24	theme	0.3	702:704	arg1	%					705:705	%	705:705	%	705:705	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	8	25	theme	coli	1178:1181	arg1	growth					1165:1170	the growth	1161:1170	the growth of E. coli, S. aureus, and Chinese chestnut fungus	1161:1221	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	7	26	theme	WVP	1082:1084	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	8	27	theme	mycelial	1239:1246	arg1	structure					1248:1256	the mycelial structure	1235:1256	the mycelial structure of the fungus	1235:1270	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	5	28	theme	%	705:705	arg1	CMA					713:715	0.3% (w/v) CMA	702:715	0.3% (w/v) CMA	702:715	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	8	29	theme	aureus	1187:1192	arg1	growth					1165:1170	the growth	1161:1170	the growth of E. coli, S. aureus, and Chinese chestnut fungus	1161:1221	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	7	30	theme	CHI	901:903	arg1	w/v					911:913	w/v	911:913	w/v	911:913	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	30	theme	CHI	901:903	arg1	%					908:908	CHI 2.5%	901:908	CHI 2.5% (w/v)	901:914	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	30	theme	CHI	901:903	arg1	formula					877:883	The optimized formula	863:883	The optimized formula of the film	863:895	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	5	31	with	%	620:620	arg1	addition					631:638	the addition	627:638	the addition of 0.5% (w/v) nano-cellulose	627:667	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	3	32	theme	composite	250:258	arg1	film					260:263	the composite film	246:263	the composite film composed of the two components	246:294	However, the composite film composed of the two components does not yield satisfactory properties for chestnut preservation.					
32519374	8	33	theme	Chinese	1199:1205	arg1	fungus					1216:1221	Chinese chestnut fungus	1199:1221	Chinese chestnut fungus	1199:1221	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	7	34	from	assessment	1067:1076	arg1	break					1127:1131	break	1127:1131	break	1127:1131	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	35	theme	film	892:895	arg1	%					908:908	CHI 2.5%	901:908	CHI 2.5% (w/v)	901:914	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	35	theme	film	892:895	arg1	formula					877:883	The optimized formula	863:883	The optimized formula of the film	863:895	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	4	36	with	film	478:481	arg1	properties					543:552	mechanical properties	532:552	mechanical properties	532:552	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	4	36	with	film	478:481	arg1	bacteriostatic					512:525	bacteriostatic	512:525	bacteriostatic	512:525	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	4	36	with	film	478:481	arg1	retention					501:509	strong water retention	488:509	strong water retention	488:509	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	5	37	theme	CMA	713:715	arg1	addition					690:697	the addition	686:697	the addition of 0.3% (w/v) CMA	686:715	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	0	38	theme	edible	39:44	arg1	film					83:86	an edible chitosan-whey protein nano composite film	36:86	an edible chitosan-whey protein nano composite film	36:86	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	8	39	theme	fungus	1216:1221	arg1	growth					1165:1170	the growth	1161:1170	the growth of E. coli, S. aureus, and Chinese chestnut fungus	1161:1221	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	5	40	theme	0.5	643:645	arg1	%					646:646	%	646:646	%	646:646	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	2	41	theme	whey	160:163	arg1	protein					165:171	whey protein	160:171	whey protein	160:171	Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation.					
32519374	7	42	theme	comprehensive	1053:1065	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	43	theme	orthogonal	1014:1023	arg1	34					1036:1037	34	1036:1037	34	1036:1037	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	43	theme	orthogonal	1014:1023	arg1	L9					1033:1034	orthogonal testing L9	1014:1034	orthogonal testing L9(34 )	1014:1039	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	44	theme	whey	917:920	arg1	w/v					936:938	w/v	936:938	w/v	936:938	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	44	theme	whey	917:920	arg1	%					933:933	whey protein 3.0%	917:933	whey protein 3.0% (w/v)	917:939	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	6	45	theme	film	794:797	arg1	density					779:785	the density	775:785	the density of the film	775:797	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
32519374	5	46	theme	%	646:646	arg1	nano-cellulose					654:667	0.5% (w/v) nano-cellulose	643:667	0.5% (w/v) nano-cellulose	643:667	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	6	47	theme	water	731:735	arg1	WS					749:750	WS	749:750	WS	749:750	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
32519374	6	47	theme	water	731:735	arg1	solubility					737:746	water solubility	731:746	water solubility (WS)	731:751	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
32519374	9	48	theme	chestnuts	1397:1405	arg1	storage					1386:1392	storage	1386:1392	storage of chestnuts at 25 °C	1386:1414	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	5	49	theme	nano-cellulose	654:667	arg1	addition					631:638	the addition	627:638	the addition of 0.5% (w/v) nano-cellulose	627:667	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	0	50	theme	protein	60:66	arg1	film					83:86	an edible chitosan-whey protein nano composite film	36:86	an edible chitosan-whey protein nano composite film	36:86	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	8	51	theme	chestnut	1207:1214	arg1	fungus					1216:1221	Chinese chestnut fungus	1199:1221	Chinese chestnut fungus	1199:1221	The film clearly inhibited the growth of E. coli, S. aureus, and Chinese chestnut fungus, destroying the mycelial structure of the fungus.					
32519374	5	52	dep	%	646:646	arg1	w/v					649:651	w/v	649:651	w/v	649:651	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	2	53	theme	edible	201:206	arg1	films					208:212	edible films	201:212	edible films	201:212	Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation.					
32519374	7	54	from	break	1127:1131	arg1	WVP					1082:1084	WVP	1082:1084	WVP	1082:1084	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	54	from	break	1127:1131	arg1	elongation					1113:1122	elongation	1113:1122	elongation	1113:1122	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	54	from	break	1127:1131	arg1	strength					1099:1106	tensile strength	1091:1106	tensile strength	1091:1106	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	54	from	break	1127:1131	arg1	WS					1087:1088	WS	1087:1088	WS	1087:1088	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	54	from	break	1127:1131	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	0	55	theme	chitosan-whey	46:58	arg1	film					83:86	an edible chitosan-whey protein nano composite film	36:86	an edible chitosan-whey protein nano composite film	36:86	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	4	56	theme	water	495:499	arg1	retention					501:509	strong water retention	488:509	strong water retention	488:509	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	7	57	theme	optimized	867:875	arg1	%					908:908	CHI 2.5%	901:908	CHI 2.5% (w/v)	901:914	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	57	theme	optimized	867:875	arg1	formula					877:883	The optimized formula	863:883	The optimized formula of the film	863:895	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	9	58	from	days	1378:1381	arg1	25 °C					1410:1414	25 °C	1410:1414	25 °C	1410:1414	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	7	59	theme	fuzzy	1047:1051	arg1	assessment					1067:1076	fuzzy comprehensive assessment	1047:1076	fuzzy comprehensive assessment	1047:1076	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	4	60	theme	strong	488:493	arg1	retention					501:509	strong water retention	488:509	strong water retention	488:509	In this study, nano-cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and mechanical properties.					
32519374	9	61	theme	calcification	1348:1360	arg1	index					1362:1366	calcification index	1348:1366	calcification index	1348:1366	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	7	62	theme	protein	922:928	arg1	w/v					936:938	w/v	936:938	w/v	936:938	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	62	theme	protein	922:928	arg1	%					933:933	whey protein 3.0%	917:933	whey protein 3.0% (w/v)	917:939	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	5	63	with	%	679:679	arg1	addition					631:638	the addition	627:638	the addition of 0.5% (w/v) nano-cellulose	627:667	The water vapor permeability (WVP) of the film decreased by 21.61% with the addition of 0.5% (w/v) nano-cellulose, and 23.02% with the addition of 0.3% (w/v) CMA.					
32519374	9	64	theme	weight	1318:1323	arg1	loss					1325:1328	the weight loss	1314:1328	the weight loss	1314:1328	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	0	65	theme	composite	73:81	arg1	film					83:86	an edible chitosan-whey protein nano composite film	36:86	an edible chitosan-whey protein nano composite film	36:86	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	6	66	theme	CMA	858:860	arg1	addition					835:842	the addition	831:842	the addition of 0.3% (w/v) CMA	831:860	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
32519374	7	67	theme	CMA	969:971	arg1	w/v					979:981	w/v	979:981	w/v	979:981	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	67	theme	CMA	969:971	arg1	%					976:976	CMA 0.3%	969:976	CMA 0.3% (w/v)	969:982	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	0	68	theme	nano	68:71	arg1	film					83:86	an edible chitosan-whey protein nano composite film	36:86	an edible chitosan-whey protein nano composite film	36:86	Development and characterization of an edible chitosan-whey protein nano composite film for chestnut (Castanea mollissima Bl.)					
32519374	7	69	theme	nano-cellulose	942:955	arg1	%					960:960	nano-cellulose 0.5%	942:960	nano-cellulose 0.5% (w/v)	942:966	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	7	69	theme	nano-cellulose	942:955	arg1	w/v					963:965	w/v	963:965	w/v	963:965	The optimized formula of the film was CHI 2.5% (w/v), whey protein 3.0% (w/v), nano-cellulose 0.5% (w/v), CMA 0.3% (w/v), and pH 3.8, as determined by orthogonal testing L9(34 ), with fuzzy comprehensive assessment, of WVP, WS, tensile strength, and elongation at break.					
32519374	2	70	theme	food	218:221	arg1	preservation					223:234	food preservation	218:234	food preservation	218:234	Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation.					
32519374	9	71	theme	mildew	1331:1336	arg1	rate					1338:1341	mildew rate	1331:1341	mildew rate	1331:1341	In addition, coating effectively reduced the weight loss, mildew rate, and calcification index during 16 days of storage of chestnuts at 25 °C.					
32519374	6	72	theme	%	850:850	arg1	CMA					858:860	0.3% (w/v) CMA	847:860	0.3% (w/v) CMA	847:860	Furthermore, water solubility (WS) decreased 22.05%, and the density of the film was significantly improved with the addition of 0.3% (w/v) CMA.					
34847186	7	0	theme	products	1105:1112	arg1	amounts					1071:1077	high amounts	1066:1077	high amounts of Fc-derived degradation products	1066:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	7	0	theme	products	1105:1112	arg1	products					1105:1112	Fc-derived degradation products	1082:1112	Fc-derived degradation products	1082:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	7	1	theme	degradation	1093:1103	arg1	products					1105:1112	Fc-derived degradation products	1082:1112	Fc-derived degradation products	1082:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	6	2	from	domain	859:864	arg1	IgG1					872:875	an IgG1	869:875	an IgG1	869:875	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	6	2	from	domain	859:864	arg1	strategy					880:887	a strategy	878:887	a strategy commonly used to improve the stability of therapeutic proteins	878:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	7	3	theme	Fc-derived	1082:1091	arg1	products					1105:1112	Fc-derived degradation products	1082:1112	Fc-derived degradation products	1082:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	11	4	theme	plant-specific	1612:1625	arg1	glycosylation					1627:1639	the plant-specific glycosylation	1608:1639	the plant-specific glycosylation	1608:1639	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	6	5	theme	Nicotiana	773:781	arg1	benthamiana					783:793	Nicotiana benthamiana	773:793	Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	773:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	11	6	theme	xylose	1811:1816	arg1	residues					1818:1825	plant core fucose and xylose residues	1789:1825	plant core fucose and xylose residues	1789:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	1	7	theme	stroke	182:187	arg1	therapy					189:195	stroke therapy	182:195	stroke therapy	182:195	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	9	8	from	mutations	1262:1270	arg1	interface					1316:1324	the Fc dimerization interface	1296:1324	the Fc dimerization interface	1296:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	6	9	theme	therapeutic	931:941	arg1	proteins					943:950	therapeutic proteins	931:950	therapeutic proteins	931:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	4	10	theme	blood	629:633	arg1	clots					635:639	blood clots	629:639	blood clots	629:639	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	10	11	theme	blood	1551:1555	arg1	clots					1557:1561	blood clots	1551:1561	blood clots	1551:1561	The ability of rPA-Fc to convert plasminogen into plasmin was demonstrated by plasminogen zymography and clot lysis assay shows that rPA-Fc is able to dissolve blood clots ex vivo.					
34847186	11	12	theme	fucose	1800:1805	arg1	residues					1818:1825	plant core fucose and xylose residues	1789:1825	plant core fucose and xylose residues	1789:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	8	13	theme	fusions	1164:1170	arg1	"					1141:1141	the "Y"	1135:1141	the "Y"	1135:1141	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	8	14	theme	physical	1232:1239	arg1	instability					1241:1251	physical instability	1232:1251	physical instability	1232:1251	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	9	15	theme	Fc-fusions	1379:1388	arg1	expression					1338:1347	the expression	1334:1347	the expression of fully stable rPA monomeric Fc-fusions	1334:1388	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	8	16	theme	steric	1178:1183	arg1	hindrance					1185:1193	steric hindrance	1178:1193	steric hindrance between protein domains	1178:1217	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	7	17	theme	rPA-Fc	1019:1024	arg1	fusions					1026:1032	dimeric rPA-Fc fusions	1011:1032	dimeric rPA-Fc fusions	1011:1032	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	11	18	theme	α1,6-core	1736:1744	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	10	19	dep	zymography	1481:1490	arg1	shows					1513:1517	shows	1513:1517	shows that rPA-Fc is able to dissolve blood clots ex vivo	1513:1569	The ability of rPA-Fc to convert plasminogen into plasmin was demonstrated by plasminogen zymography and clot lysis assay shows that rPA-Fc is able to dissolve blood clots ex vivo.					
34847186	2	20	theme	cleaved	245:251	arg1	enzyme					281:286	a proteolytic enzyme	267:286	a proteolytic enzyme that breaks the crosslinks between fibrin molecules	267:338	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	2	20	theme	cleaved	245:251	arg1	plasmin					258:264	its cleaved form plasmin	241:264	its cleaved form plasmin	241:264	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	11	21	theme	plant	1789:1793	arg1	fucose					1800:1805	plant core fucose	1789:1805	plant core fucose	1789:1805	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	0	22	theme	blood	69:73	arg1	clots					75:79	blood clots	69:79	blood clots	69:79	Reteplase Fc-fusions produced in N. benthamiana are able to dissolve blood clots ex vivo.					
34847186	3	23	theme	tissue	364:369	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	23	theme	tissue	364:369	arg1	rPA					404:406	rPA	404:406	rPA	404:406	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	23	theme	tissue	364:369	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	4	24	theme	Alteplase	502:510	arg1	mutant					478:483	a deletion mutant	467:483	a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots	467:639	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	4	24	theme	Alteplase	502:510	arg1	rPA					460:462	rPA	460:462	rPA	460:462	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	11	25	theme	devoid	1779:1784	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	3	26	theme	FDA-approved	345:356	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	26	theme	FDA-approved	345:356	arg1	rPA					404:406	rPA	404:406	rPA	404:406	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	26	theme	FDA-approved	345:356	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	9	27	theme	critical	1275:1282	arg1	residues					1284:1291	critical residues	1275:1291	critical residues in the Fc dimerization interface	1275:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	0	28	theme	Reteplase	0:8	arg1	Fc-fusions					10:19	Reteplase Fc-fusions	0:19	Reteplase Fc-fusions produced in N. benthamiana	0:46	Reteplase Fc-fusions produced in N. benthamiana are able to dissolve blood clots ex vivo.					
34847186	11	29	dep	addressed	1584:1592	arg1	concerns					1594:1601	concerns	1594:1601	addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1584:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	3	30	theme	activator	383:391	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	30	theme	activator	383:391	arg1	rPA					404:406	rPA	404:406	rPA	404:406	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	30	theme	activator	383:391	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	4	31	theme	plasma	543:548	arg1	half-life					550:558	an extended plasma half-life	531:558	an extended plasma half-life	531:558	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	10	32	theme	plasminogen	1469:1479	arg1	zymography					1481:1490	plasminogen zymography	1469:1490	plasminogen zymography	1469:1490	The ability of rPA-Fc to convert plasminogen into plasmin was demonstrated by plasminogen zymography and clot lysis assay shows that rPA-Fc is able to dissolve blood clots ex vivo.					
34847186	11	33	gly	fucosylated	1746:1756	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	9	34	theme	Fc	1300:1301	arg1	interface					1316:1324	the Fc dimerization interface	1296:1324	the Fc dimerization interface	1296:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	11	35	theme	serum-like	1684:1693	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	11	35	theme	serum-like	1684:1693	arg1	structures					1695:1704	serum-like structures	1684:1704	serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1684:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	0	36	theme	N.	33:34	arg1	benthamiana					36:46	N. benthamiana	33:46	N. benthamiana	33:46	Reteplase Fc-fusions produced in N. benthamiana are able to dissolve blood clots ex vivo.					
34847186	10	37	theme	lysis	1501:1505	arg1	assay					1507:1511	clot lysis assay	1496:1511	clot lysis assay	1496:1511	The ability of rPA-Fc to convert plasminogen into plasmin was demonstrated by plasminogen zymography and clot lysis assay shows that rPA-Fc is able to dissolve blood clots ex vivo.					
34847186	6	38	theme	fragment	830:837	arg1	domain					859:864	the immunoglobulin fragment crystallizable (Fc) domain	811:864	the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	811:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	2	39	theme	Thrombolytic	198:209	arg1	drugs					211:215	Thrombolytic drugs	198:215	Thrombolytic drugs	198:215	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	9	40	theme	stable	1358:1363	arg1	Fc-fusions					1379:1388	fully stable rPA monomeric Fc-fusions	1352:1388	fully stable rPA monomeric Fc-fusions	1352:1388	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	5	41	theme	Different	642:650	arg1	methods					652:658	Different methods	642:658	Different methods	642:658	Different methods have been proposed to improve the production of rPA.					
34847186	9	42	theme	monomeric	1369:1377	arg1	Fc-fusions					1379:1388	fully stable rPA monomeric Fc-fusions	1352:1388	fully stable rPA monomeric Fc-fusions	1352:1388	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	4	43	theme	fibrin	569:574	arg1	specificity					576:586	fibrin specificity	569:586	fibrin specificity	569:586	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	6	44	theme	rPA	798:800	arg1	benthamiana					783:793	Nicotiana benthamiana	773:793	Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	773:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	7	45	theme	high	1066:1069	arg1	amounts					1071:1077	high amounts	1066:1077	high amounts of Fc-derived degradation products	1066:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	7	45	theme	high	1066:1069	arg1	products					1105:1112	Fc-derived degradation products	1082:1112	Fc-derived degradation products	1082:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	2	46	theme	proteolytic	269:279	arg1	enzyme					281:286	a proteolytic enzyme	267:286	a proteolytic enzyme that breaks the crosslinks between fibrin molecules	267:338	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	2	46	theme	proteolytic	269:279	arg1	plasmin					258:264	its cleaved form plasmin	241:264	its cleaved form plasmin	241:264	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	1	47	theme	blood	167:171	arg1	clots					173:177	blood clots	167:177	blood clots in stroke therapy	167:195	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	8	48	theme	Y	1140:1140	arg1	"					1141:1141	the "Y"	1135:1141	the "Y"	1135:1141	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	9	49	from	residues	1284:1291	arg1	interface					1316:1324	the Fc dimerization interface	1296:1324	the Fc dimerization interface	1296:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	6	50	theme	proteins	943:950	arg1	stability					918:926	the stability	914:926	the stability of therapeutic proteins	914:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	8	51	theme	protein	1203:1209	arg1	domains					1211:1217	protein domains	1203:1217	protein domains	1203:1217	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	11	52	theme	residues	1818:1825	arg1	devoid					1779:1784	devoid	1779:1784	devoid	1779:1784	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	7	53	theme	protein	1035:1041	arg1	instability					1043:1053	protein instability	1035:1053	protein instability	1035:1053	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	6	54	theme	transient	749:757	arg1	expression					759:768	the transient expression	745:768	the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	745:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	7	55	theme	fusions	1026:1032	arg1	purification					995:1006	purification	995:1006	purification	995:1006	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	7	55	theme	fusions	1026:1032	arg1	expression					980:989	expression	980:989	expression	980:989	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	7	56	from	success	965:971	arg1	purification					995:1006	purification	995:1006	purification	995:1006	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	7	56	from	success	965:971	arg1	expression					980:989	expression	980:989	expression	980:989	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	8	57	theme	Fc	1161:1162	arg1	fusions					1164:1170	dimeric Fc fusions	1153:1170	dimeric Fc fusions	1153:1170	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	8	58	theme	dimeric	1153:1159	arg1	fusions					1164:1170	dimeric Fc fusions	1153:1170	dimeric Fc fusions	1153:1170	We hypothesize that the "Y"- shape of dimeric Fc fusions cause steric hindrance between protein domains and leads to physical instability.					
34847186	7	59	theme	dimeric	1011:1017	arg1	fusions					1026:1032	dimeric rPA-Fc fusions	1011:1032	dimeric rPA-Fc fusions	1011:1032	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	11	60	gly	α2,6-sialylated	1716:1730	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	2	61	theme	form	253:256	arg1	enzyme					281:286	a proteolytic enzyme	267:286	a proteolytic enzyme that breaks the crosslinks between fibrin molecules	267:338	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	2	61	theme	form	253:256	arg1	plasmin					258:264	its cleaved form plasmin	241:264	its cleaved form plasmin	241:264	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	1	62	theme	effective	134:142	arg1	treatments					144:153	effective treatments	134:153	effective treatments	134:153	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	1	62	theme	effective	134:142	arg1	fibrinolytic					107:118	fibrinolytic	107:118	fibrinolytic	107:118	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	1	62	theme	effective	134:142	arg1	Thrombolytic					90:101	Thrombolytic	90:101	Thrombolytic	90:101	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	4	63	theme	wild-type	492:500	arg1	Alteplase					502:510	the wild-type Alteplase	488:510	the wild-type Alteplase	488:510	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	1	64	dep	Thrombolytic	90:101	arg1	therapies					120:128	therapies	120:128	therapies	120:128	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	11	65	theme	fucosylated	1746:1756	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	3	66	gly	non-glycosylated	414:429	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	66	gly	non-glycosylated	414:429	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	1	67	from	clots	173:177	arg1	therapy					189:195	stroke therapy	182:195	stroke therapy	182:195	Thrombolytic and fibrinolytic therapies are effective treatments to dissolve blood clots in stroke therapy.					
34847186	10	68	theme	rPA-Fc	1406:1411	arg1	ability					1395:1401	The ability	1391:1401	The ability of rPA-Fc to convert plasminogen into plasmin	1391:1447	The ability of rPA-Fc to convert plasminogen into plasmin was demonstrated by plasminogen zymography and clot lysis assay shows that rPA-Fc is able to dissolve blood clots ex vivo.					
34847186	11	69	theme	core	1795:1798	arg1	fucose					1800:1805	plant core fucose	1789:1805	plant core fucose	1789:1805	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	9	70	from	interface	1316:1324	arg1	mutations					1262:1270	mutations	1262:1270	mutations of critical residues in the Fc dimerization interface	1262:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	3	71	theme	plasminogen	371:381	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	71	theme	plasminogen	371:381	arg1	rPA					404:406	rPA	404:406	rPA	404:406	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	71	theme	plasminogen	371:381	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	4	72	theme	deletion	469:476	arg1	mutant					478:483	a deletion mutant	467:483	a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots	467:639	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	4	72	theme	deletion	469:476	arg1	rPA					460:462	rPA	460:462	rPA	460:462	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	3	73	theme	human	358:362	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	73	theme	human	358:362	arg1	rPA					404:406	rPA	404:406	rPA	404:406	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	73	theme	human	358:362	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	7	74	dep	expression	980:989	arg1	the					976:978	the	976:978	the	976:978	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	9	75	theme	residues	1284:1291	arg1	mutations					1262:1270	mutations	1262:1270	mutations of critical residues in the Fc dimerization interface	1262:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	5	76	theme	rPA	708:710	arg1	production					694:703	the production	690:703	the production of rPA	690:710	Different methods have been proposed to improve the production of rPA.					
34847186	4	77	from	half-life	550:558	arg1	benefits					517:524	benefits	517:524	benefits from an extended plasma half-life	517:558	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	6	78	from	expression	759:768	arg1	benthamiana					783:793	Nicotiana benthamiana	773:793	Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	773:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	7	79	link	Fc-derived	1082:1091	arg1	products					1105:1112	Fc-derived degradation products	1082:1112	Fc-derived degradation products	1082:1112	Despite our success on the expression and purification of dimeric rPA-Fc fusions, protein instability results in high amounts of Fc-derived degradation products.					
34847186	9	80	theme	dimerization	1303:1314	arg1	interface					1316:1324	the Fc dimerization interface	1296:1324	the Fc dimerization interface	1296:1324	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
34847186	6	81	theme	Fc	855:856	arg1	domain					859:864	the immunoglobulin fragment crystallizable (Fc) domain	811:864	the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	811:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	3	82	theme	non-glycosylated	414:429	arg1	protein					431:437	a non-glycosylated protein	412:437	a non-glycosylated protein produced in E. coli	412:457	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	3	82	theme	non-glycosylated	414:429	arg1	Reteplase					393:401	The FDA-approved human tissue plasminogen activator Reteplase	341:401	The FDA-approved human tissue plasminogen activator Reteplase (rPA)	341:407	The FDA-approved human tissue plasminogen activator Reteplase (rPA) is a non-glycosylated protein produced in E. coli.					
34847186	11	83	theme	rPA-Fc	1655:1660	arg1	glycosylation					1662:1674	rPA-Fc glycosylation	1655:1674	rPA-Fc glycosylation	1655:1674	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	6	84	theme	crystallizable	839:852	arg1	domain					859:864	the immunoglobulin fragment crystallizable (Fc) domain	811:864	the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	811:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	4	85	theme	extended	534:541	arg1	half-life					550:558	an extended plasma half-life	531:558	an extended plasma half-life	531:558	rPA is a deletion mutant of the wild-type Alteplase that benefits from an extended plasma half-life, reduced fibrin specificity and the ability to better penetrate into blood clots.					
34847186	10	86	theme	clot	1496:1499	arg1	lysis					1501:1505	clot lysis	1496:1505	clot lysis assay	1496:1511	The ability of rPA-Fc to convert plasminogen into plasmin was demonstrated by plasminogen zymography and clot lysis assay shows that rPA-Fc is able to dissolve blood clots ex vivo.					
34847186	11	87	theme	α2,6-sialylated	1716:1730	arg1	N-glycans					1758:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans	1716:1766	α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues	1716:1825	Finally, we addressed concerns with the plant-specific glycosylation by modulating rPA-Fc glycosylation towards serum-like structures including α2,6-sialylated and α1,6-core fucosylated N-glycans completely devoid of plant core fucose and xylose residues.					
34847186	6	88	theme	first	734:738	arg1	time					740:743	the first time	730:743	the first time	730:743	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	6	89	theme	immunoglobulin	815:828	arg1	domain					859:864	the immunoglobulin fragment crystallizable (Fc) domain	811:864	the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins	811:950	Here we show for the first time the transient expression in Nicotiana benthamiana of rPA fused to the immunoglobulin fragment crystallizable (Fc) domain on an IgG1, a strategy commonly used to improve the stability of therapeutic proteins.					
34847186	2	90	theme	fibrin	323:328	arg1	molecules					330:338	fibrin molecules	323:338	fibrin molecules	323:338	Thrombolytic drugs activate plasminogen to its cleaved form plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules.					
34847186	9	91	theme	rPA	1365:1367	arg1	Fc-fusions					1379:1388	fully stable rPA monomeric Fc-fusions	1352:1388	fully stable rPA monomeric Fc-fusions	1352:1388	Indeed, mutations of critical residues in the Fc dimerization interface allowed the expression of fully stable rPA monomeric Fc-fusions.					
32691500	6	0	theme	multifunctional	875:889	arg1	mats					897:900	The multifunctional fiber mats	871:900	The multifunctional fiber mats	871:900	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	6	1	theme	MPa	1002:1004	arg1	strength					986:993	ultimate tensile strength	969:993	ultimate tensile strength of 3-5 MPa	969:1004	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	1	2	theme	matrix	247:252	arg1	one					222:224	the one	218:224	the one of the extracellular matrix of the skin	218:264	Wound dressings produced by electrospinning exhibit a fibrous structure close to the one of the extracellular matrix of the skin.					
32691500	0	3	theme	wound	71:75	arg1	dressing					77:84	electrospun wound dressing	59:84	electrospun wound dressing	59:84	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	7	4	theme	bioactive	1130:1138	arg1	glass					1140:1144	bioactive glass	1130:1144	bioactive glass	1130:1144	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	8	5	theme	human	1229:1233	arg1	cells					1260:1264	human keratinocytes-like HaCaT cells	1229:1264	human keratinocytes-like HaCaT cells	1229:1264	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	7	6	theme	improved	1072:1079	arg1	bioactivity					1081:1091	the improved bioactivity	1068:1091	the improved bioactivity of the fiber mats by the addition of bioactive glass	1068:1144	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	6	7	dep	properties	957:966	arg1	strength					986:993	ultimate tensile strength	969:993	ultimate tensile strength of 3-5 MPa	969:1004	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	6	8	theme	improved	912:919	arg1	wettability					921:931	improved wettability	912:931	improved wettability	912:931	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	9	9	theme	infected	1650:1657	arg1	wounds					1659:1664	infected wounds	1650:1664	infected wounds	1650:1664	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	9	10	theme	antibacterial	1495:1507	arg1	tests					1509:1513	the antibacterial tests	1491:1513	the antibacterial tests	1491:1513	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	8	11	theme	wound	1340:1344	arg1	dressing					1346:1353	wound dressing	1340:1353	wound dressing	1340:1353	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	1	12	theme	skin	261:264	arg1	matrix					247:252	the extracellular matrix	229:252	the extracellular matrix of the skin	229:264	Wound dressings produced by electrospinning exhibit a fibrous structure close to the one of the extracellular matrix of the skin.					
32691500	5	13	theme	Morphological	704:716	arg1	examinations					731:742	Morphological and chemical examinations	704:742	Morphological and chemical examinations	704:742	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	8	14	theme	fiber	1315:1319	arg1	mats					1321:1324	the fabricated composite fiber mats	1290:1324	the fabricated composite fiber mats	1290:1324	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	7	15	theme	mats	1106:1109	arg1	bioactivity					1081:1091	the improved bioactivity	1068:1091	the improved bioactivity of the fiber mats by the addition of bioactive glass	1068:1144	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	3	16	used	used	519:522	arg2	platform					543:550	a biodegradable platform	527:550	a biodegradable platform to deliver bioactive glass particles	527:587	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	3	16	used	used	519:522	arg2	mats					460:463	The blend fiber mats	444:463	The blend fiber mats	444:463	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	3	17	theme	Manuka	489:494	arg1	honey					496:500	Manuka honey	489:500	Manuka honey	489:500	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	4	18	theme	therapeutic	622:632	arg1	effect					634:639	a dual therapeutic effect	615:639	a dual therapeutic effect	615:639	It was hypothesized that a dual therapeutic effect can be achieved by combining Manuka honey and bioactive glass.					
32691500	0	19	theme	bioactive	103:111	arg1	glass					113:117	bioactive glass and Manuka honey	103:134	glass	113:117	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	6	20	theme	fiber	891:895	arg1	mats					897:900	The multifunctional fiber mats	871:900	The multifunctional fiber mats	871:900	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	9	21	theme	wounds	1659:1664	arg1	treatment					1637:1645	the treatment	1633:1645	the treatment of infected wounds	1633:1664	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	9	22	theme	tests	1509:1513	arg1	results					1480:1486	the results	1476:1486	the results of the antibacterial tests	1476:1513	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	4	23	theme	dual	617:620	arg1	effect					634:639	a dual therapeutic effect	615:639	a dual therapeutic effect	615:639	It was hypothesized that a dual therapeutic effect can be achieved by combining Manuka honey and bioactive glass.					
32691500	5	24	theme	PCL-MC	798:803	arg1	mats					811:814	submicrometric PCL-MC fiber mats	783:814	submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles	783:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	6	25	theme	tensile	978:984	arg1	strength					986:993	ultimate tensile strength	969:993	ultimate tensile strength of 3-5 MPa	969:1004	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	5	26	theme	chemical	722:729	arg1	examinations					731:742	Morphological and chemical examinations	704:742	Morphological and chemical examinations	704:742	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	5	27	theme	fiber	805:809	arg1	mats					811:814	submicrometric PCL-MC fiber mats	783:814	submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles	783:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	7	28	theme	fiber	1100:1104	arg1	mats					1106:1109	the fiber mats	1096:1109	the fiber mats	1096:1109	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	5	29	theme	glass	854:858	arg1	particles					860:868	bioactive glass particles	844:868	bioactive glass particles	844:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	3	30	theme	biodegradable	529:541	arg1	platform					543:550	a biodegradable platform	527:550	a biodegradable platform to deliver bioactive glass particles	527:587	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	3	30	theme	biodegradable	529:541	arg1	mats					460:463	The blend fiber mats	444:463	The blend fiber mats	444:463	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	1	31	theme	fibrous	191:197	arg1	structure					199:207	a fibrous structure	189:207	a fibrous structure close to the one of the extracellular matrix of the skin	189:264	Wound dressings produced by electrospinning exhibit a fibrous structure close to the one of the extracellular matrix of the skin.					
32691500	0	32	theme	novel	16:20	arg1	ɛ-caprolactone					27:40	ɛ-caprolactone	27:40	ɛ-caprolactone	27:40	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	0	32	theme	novel	16:20	arg1	poly					22:25	a novel poly	14:25	a novel poly(ɛ-caprolactone)	14:41	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	8	33	theme	cell	1175:1178	arg1	tests					1188:1192	cell biology tests	1175:1192	cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells	1175:1264	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	0	34	theme	Manuka	123:128	arg1	honey					130:134	bioactive glass and Manuka honey	103:134	honey	130:134	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	8	35	theme	dermal	1206:1211	arg1	fibroblasts					1213:1223	human dermal fibroblasts	1200:1223	human dermal fibroblasts	1200:1223	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	9	36	theme	electrospun	1559:1569	arg1	mats					1577:1580	the electrospun fiber mats	1555:1580	the electrospun fiber mats	1555:1580	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	9	37	theme	mats	1577:1580	arg1	optimization					1539:1550	an optimization	1536:1550	an optimization of the electrospun fiber mats	1536:1580	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	5	38	theme	Manuka	827:832	arg1	honey					834:838	Manuka honey	827:838	Manuka honey	827:838	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	8	39	theme	human	1200:1204	arg1	fibroblasts					1213:1223	human dermal fibroblasts	1200:1223	human dermal fibroblasts	1200:1223	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	5	40	contain	containing	816:825	arg2	particles					860:868	bioactive glass particles	844:868	bioactive glass particles	844:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	5	40	contain	containing	816:825	arg1	mats					811:814	submicrometric PCL-MC fiber mats	783:814	submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles	783:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	5	40	contain	containing	816:825	arg2	honey					834:838	Manuka honey	827:838	Manuka honey	827:838	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	9	41	theme	fiber	1571:1575	arg1	mats					1577:1580	the electrospun fiber mats	1555:1580	the electrospun fiber mats	1555:1580	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	1	42	theme	Wound	137:141	arg1	dressings					143:151	Wound dressings	137:151	Wound dressings produced by electrospinning	137:179	Wound dressings produced by electrospinning exhibit a fibrous structure close to the one of the extracellular matrix of the skin.					
32691500	5	43	theme	successful	758:767	arg1	production					769:778	the successful production	754:778	the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles	754:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	3	44	theme	fiber	454:458	arg1	platform					543:550	a biodegradable platform	527:550	a biodegradable platform to deliver bioactive glass particles	527:587	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	3	44	theme	fiber	454:458	arg1	mats					460:463	The blend fiber mats	444:463	The blend fiber mats	444:463	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	8	45	theme	bioactive	1440:1448	arg1	glass					1450:1454	bioactive glass	1440:1454	bioactive glass	1440:1454	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	8	46	theme	wound	1396:1400	arg1	closure					1402:1408	wound closure	1396:1408	wound closure influenced by the presence of bioactive glass	1396:1454	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	8	47	theme	fabricated	1294:1303	arg1	mats					1321:1324	the fabricated composite fiber mats	1290:1324	the fabricated composite fiber mats	1290:1324	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	0	48	theme	poly	22:25	arg1	Production					0:9	Production	0:9	Production of a novel poly(ɛ-caprolactone)	0:41	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	6	49	theme	ultimate	969:976	arg1	strength					986:993	ultimate tensile strength	969:993	ultimate tensile strength of 3-5 MPa	969:1004	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	2	50	theme	benign	427:432	arg1	solvents					434:441	benign solvents	427:441	benign solvents	427:441	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	4	51	theme	Manuka	670:675	arg1	honey					677:681	Manuka honey	670:681	Manuka honey	670:681	It was hypothesized that a dual therapeutic effect can be achieved by combining Manuka honey and bioactive glass.					
32691500	3	52	theme	bioactive	563:571	arg1	particles					579:587	bioactive glass particles	563:587	bioactive glass particles	563:587	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	8	53	theme	glass	1450:1454	arg1	presence					1428:1435	the presence	1424:1435	the presence of bioactive glass	1424:1454	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	5	54	theme	mats	811:814	arg1	production					769:778	the successful production	754:778	the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles	754:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	7	55	theme	glass	1140:1144	arg1	addition					1118:1125	the addition	1114:1125	the addition of bioactive glass	1114:1144	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	3	56	theme	blend	448:452	arg1	platform					543:550	a biodegradable platform	527:550	a biodegradable platform to deliver bioactive glass particles	527:587	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	3	56	theme	blend	448:452	arg1	mats					460:463	The blend fiber mats	444:463	The blend fiber mats	444:463	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	5	57	theme	submicrometric	783:796	arg1	mats					811:814	submicrometric PCL-MC fiber mats	783:814	submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles	783:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	9	58	theme	wound	1614:1618	arg1	dressing					1620:1627	suitable wound dressing	1605:1627	suitable wound dressing for the treatment of infected wounds	1605:1664	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	2	59	theme	fiber	322:326	arg1	mats					328:331	fiber mats	322:331	fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents	322:441	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	6	60	theme	mechanical	946:955	arg1	properties					957:966	suitable mechanical properties	937:966	suitable mechanical properties (ultimate tensile strength of 3-5 MPa)	937:1005	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	7	61	theme	simulated	1046:1054	arg1	fluid					1061:1065	simulated body fluid	1046:1065	simulated body fluid	1046:1065	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	7	62	theme	dissolution	1022:1032	arg1	tests					1034:1038	dissolution tests	1022:1038	dissolution tests using simulated body fluid	1022:1065	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	4	63	theme	bioactive	687:695	arg1	glass					697:701	bioactive glass	687:701	bioactive glass	687:701	It was hypothesized that a dual therapeutic effect can be achieved by combining Manuka honey and bioactive glass.					
32691500	9	64	theme	suitable	1605:1612	arg1	dressing					1620:1627	suitable wound dressing	1605:1627	suitable wound dressing for the treatment of infected wounds	1605:1664	Moreover, based on the results of the antibacterial tests, it is apparent that an optimization of the electrospun fiber mats is required to develop suitable wound dressing for the treatment of infected wounds.					
32691500	7	65	theme	body	1056:1059	arg1	fluid					1061:1065	simulated body fluid	1046:1065	simulated body fluid	1046:1065	By performing dissolution tests using simulated body fluid, the improved bioactivity of the fiber mats by the addition of bioactive glass was confirmed.					
32691500	8	66	theme	HaCaT	1254:1258	arg1	cells					1260:1264	human keratinocytes-like HaCaT cells	1229:1264	human keratinocytes-like HaCaT cells	1229:1264	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	2	67	dep	biopolymers	357:367	arg1	ɛ-caprolactone					374:387	ɛ-caprolactone	374:387	ɛ-caprolactone	374:387	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	67	dep	biopolymers	357:367	arg1	methylcellulose					400:414	methylcellulose	400:414	methylcellulose (MC)	400:419	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	67	dep	biopolymers	357:367	arg1	MC					417:418	MC	417:418	MC	417:418	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	67	dep	biopolymers	357:367	arg1	PCL					391:393	PCL	391:393	PCL	391:393	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	67	dep	biopolymers	357:367	arg1	biopolymers					357:367	the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC)	342:419	the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents	342:441	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	67	dep	biopolymers	357:367	arg1	poly					369:372	poly	369:372	poly(ɛ-caprolactone) (PCL)	369:394	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	8	68	theme	mats	1321:1324	arg1	potential					1277:1285	the potential	1273:1285	the potential of the fabricated composite fiber mats to be used as wound dressing	1273:1353	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	0	69	theme	electrospun	59:69	arg1	dressing					77:84	electrospun wound dressing	59:84	electrospun wound dressing	59:84	Production of a novel poly(ɛ-caprolactone)-methylcellulose electrospun wound dressing by incorporating bioactive glass and Manuka honey.					
32691500	5	70	theme	bioactive	844:852	arg1	particles					860:868	bioactive glass particles	844:868	bioactive glass particles	844:868	Morphological and chemical examinations confirmed the successful production of submicrometric PCL-MC fiber mats containing Manuka honey and bioactive glass particles.					
32691500	3	71	theme	glass	573:577	arg1	particles					579:587	bioactive glass particles	563:587	bioactive glass particles	563:587	The blend fiber mats were cross-linked using Manuka honey and additionally used as a biodegradable platform to deliver bioactive glass particles.					
32691500	8	72	theme	biology	1180:1186	arg1	tests					1188:1192	cell biology tests	1175:1192	cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells	1175:1264	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	2	73	theme	well-known	346:355	arg1	biopolymers					357:367	the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC)	342:419	the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents	342:441	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	73	theme	well-known	346:355	arg1	poly					369:372	poly	369:372	poly(ɛ-caprolactone) (PCL)	369:394	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	73	theme	well-known	346:355	arg1	methylcellulose					400:414	methylcellulose	400:414	methylcellulose (MC)	400:419	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	2	74	used	used	304:307	arg2	electrospinning					284:298	electrospinning	284:298	electrospinning	284:298	In this article, electrospinning was used to fabricate fiber mats based on the well-known biopolymers poly(ɛ-caprolactone) (PCL) and methylcellulose (MC) using benign solvents.					
32691500	8	75	theme	keratinocytes-like	1235:1252	arg1	cells					1260:1264	human keratinocytes-like HaCaT cells	1229:1264	human keratinocytes-like HaCaT cells	1229:1264	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	6	76	theme	suitable	937:944	arg1	properties					957:966	suitable mechanical properties	937:966	suitable mechanical properties (ultimate tensile strength of 3-5 MPa)	937:1005	The multifunctional fiber mats exhibited improved wettability and suitable mechanical properties (ultimate tensile strength of 3-5 MPa).					
32691500	8	77	theme	composite	1305:1313	arg1	mats					1321:1324	the fabricated composite fiber mats	1290:1324	the fabricated composite fiber mats	1290:1324	Additionally, cell biology tests using human dermal fibroblasts and human keratinocytes-like HaCaT cells showed the potential of the fabricated composite fiber mats to be used as wound dressing, specially due to the ability to support wound closure influenced by the presence of bioactive glass.					
32691500	1	78	theme	extracellular	233:245	arg1	matrix					247:252	the extracellular matrix	229:252	the extracellular matrix of the skin	229:264	Wound dressings produced by electrospinning exhibit a fibrous structure close to the one of the extracellular matrix of the skin.					
34531497	1	0	theme	0.5	251:253	arg1	%					254:254	%	254:254	%	254:254	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	9	1	theme	fresh	1561:1565	arg1	commodities					1573:1583	fresh fruit commodities	1561:1583	fresh fruit commodities	1561:1583	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	7	2	theme	light	1292:1296	arg1	wavelengths					1298:1308	UV and visible light wavelengths	1277:1308	wavelengths	1298:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	3	3	theme	regular	595:601	arg1	emulsion					603:610	(4) regular emulsion	591:610	(4) regular emulsion	591:610	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	1	4	theme	sandalwood	256:265	arg1	SEO					272:274	SEO	272:274	SEO	272:274	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	1	4	theme	sandalwood	256:265	arg1	oil					267:269	0.5% sandalwood oil	251:269	0.5% sandalwood oil (SEO)	251:275	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	4	5	theme	droplet	727:733	arg1	diameter					735:742	droplet diameter	727:742	droplet diameter	727:742	Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology.					
34531497	2	6	theme	oil-in-water	341:352	arg1	emulsion					364:371	oil-in-water Pickering emulsion	341:371	oil-in-water Pickering emulsion	341:371	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	7	7	theme	tensile	1314:1320	arg1	strength					1322:1329	tensile strength	1314:1329	tensile strength	1314:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	3	8	theme	unstable	510:517	arg1	emulsion					519:526	(2) unstable emulsion	506:526	(2) unstable emulsion (0.006-0.21% CNFs)	506:545	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	8	theme	unstable	510:517	arg1	CNFs					541:544	0.006-0.21% CNFs	529:544	0.006-0.21% CNFs	529:544	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	7	9	theme	light	1255:1259	arg1	transmission					1261:1272	light transmission	1255:1272	light transmission at UV and visible light wavelengths	1255:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	10	theme	CS	1242:1243	arg1	strength					1322:1329	tensile strength	1314:1329	tensile strength	1314:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	10	theme	CS	1242:1243	arg1	properties					1210:1219	the functional properties	1195:1219	the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength	1195:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	10	theme	CS	1242:1243	arg1	transmission					1261:1272	light transmission	1255:1272	light transmission at UV and visible light wavelengths	1255:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	0	11	theme	fruit	152:156	arg1	application					158:168	fresh fruit application	146:168	fresh fruit application	146:168	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	3	12	theme	%	539:539	arg1	emulsion					519:526	(2) unstable emulsion	506:526	(2) unstable emulsion (0.006-0.21% CNFs)	506:545	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	12	theme	%	539:539	arg1	CNFs					541:544	0.006-0.21% CNFs	529:544	0.006-0.21% CNFs	529:544	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	1	13	theme	%	231:231	arg1	CS					243:244	CS	243:244	CS	243:244	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	1	13	theme	%	231:231	arg1	chitosan					233:240	0.8% chitosan	228:240	0.8% chitosan (CS)	228:245	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	3	14	theme	surfactant	633:642	arg1	addition					621:628	the addition	617:628	the addition of surfactant	617:642	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	8	15	theme	Atomic	1332:1337	arg1	microscopy					1345:1354	Atomic force microscopy	1332:1354	Atomic force microscopy	1332:1354	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	7	16	theme	functional	1199:1208	arg1	strength					1322:1329	tensile strength	1314:1329	tensile strength	1314:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	16	theme	functional	1199:1208	arg1	properties					1210:1219	the functional properties	1195:1219	the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength	1195:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	16	theme	functional	1199:1208	arg1	transmission					1261:1272	light transmission	1255:1272	light transmission at UV and visible light wavelengths	1255:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	6	17	dep	vitro	1097:1101	arg1	changes					1136:1142	changes	1136:1142	changes	1136:1142	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	0	18	theme	emulsion	72:79	arg1	coating					81:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	8	19	theme	scanning	1360:1367	arg1	microscopy					1378:1387	scanning electron microscopy	1360:1387	scanning electron microscopy	1360:1387	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	1	20	theme	composite	179:187	arg1	edible					189:194	A novel composite edible	171:194	A novel composite edible coating film	171:207	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	6	21	theme	assays	1086:1091	arg1	series					1076:1081	a series	1074:1081	a series of assays (in vitro, in vivo, and membrane integrity changes)	1074:1143	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	0	22	theme	appropriate	103:113	arg1	dosage					135:140	appropriate cellulose nanofiber dosage	103:140	appropriate cellulose nanofiber dosage for fresh fruit application	103:168	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	4	23	theme	laser	654:658	arg1	microscopy					669:678	Confocal laser scanning microscopy	645:678	Confocal laser scanning microscopy	645:678	Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology.					
34531497	2	24	theme	emulsion	364:371	arg1	nanofibers					288:297	Cellulose nanofibers	278:297	Cellulose nanofibers (CNFs)	278:304	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	2	24	theme	emulsion	364:371	arg1	agent					332:336	a stabilizer agent	319:336	a stabilizer agent of oil-in-water Pickering emulsion	319:371	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	0	25	theme	nanofiber	125:133	arg1	dosage					135:140	appropriate cellulose nanofiber dosage	103:140	appropriate cellulose nanofiber dosage for fresh fruit application	103:168	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	3	26	theme	typical	388:394	arg1	stabilization					435:447	CNF level-dependent emulsion stabilization	406:447	CNF level-dependent emulsion stabilization	406:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	26	theme	typical	388:394	arg1	groups					396:401	four typical groups	383:401	four typical groups of CNF level-dependent emulsion stabilization	383:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	0	27	theme	Antifungal	0:9	arg1	features					11:18	Antifungal features	0:18	Antifungal features	0:18	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	3	28	dep	emulsion	473:480	arg1	1					461:461	1	461:461	1	461:461	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	2	29	used	used	311:314	arg2	agent					332:336	a stabilizer agent	319:336	a stabilizer agent of oil-in-water Pickering emulsion	319:371	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	2	29	used	used	311:314	arg2	CNFs					300:303	CNFs	300:303	CNFs	300:303	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	2	29	used	used	311:314	arg2	nanofibers					288:297	Cellulose nanofibers	278:297	Cellulose nanofibers (CNFs)	278:304	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	3	30	theme	stabilization	435:447	arg1	stabilization					435:447	CNF level-dependent emulsion stabilization	406:447	CNF level-dependent emulsion stabilization	406:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	30	theme	stabilization	435:447	arg1	groups					396:401	four typical groups	383:401	four typical groups of CNF level-dependent emulsion stabilization	383:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	9	31	theme	Emulsion-CNF	1474:1485	arg1	coating					1498:1504	Emulsion-CNF stabilized coating	1474:1504	Emulsion-CNF stabilized coating	1474:1504	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	7	32	theme	CNFs	1167:1170	arg1	incorporation					1150:1162	The incorporation	1146:1162	The incorporation of CNFs	1146:1170	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	6	33	dep	improve	965:971	arg1	confirmed					1059:1067	confirmed	1059:1067	may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes)	961:1143	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	3	34	theme	level-dependent	410:424	arg1	stabilization					435:447	CNF level-dependent emulsion stabilization	406:447	CNF level-dependent emulsion stabilization	406:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	6	35	theme	effective	921:929	arg1	concentration					931:943	The effective concentration	917:943	The effective concentration of CNFs (0.24%)	917:959	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	8	36	theme	film	1456:1459	arg1	formulation					1461:1471	each coating film formulation	1443:1471	each coating film formulation	1443:1471	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	0	37	theme	oil	58:60	arg1	coating					81:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	6	38	theme	coating	995:1001	arg1	performance					977:987	the performance	973:987	the performance of CS coating	973:1001	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	9	39	theme	active	1542:1547	arg1	coating					1549:1555	active coating	1542:1555	active coating	1542:1555	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	3	40	theme	unstable	464:471	arg1	emulsion					473:480	(1) unstable emulsion	460:480	(1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion	460:610	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	4	41	theme	morphology	748:757	arg1	characteristics					708:722	the characteristics	704:722	the characteristics of droplet diameter and morphology	704:757	Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology.					
34531497	3	42	from	emulsion	473:480	arg1	emulsion					603:610	(4) regular emulsion	591:610	(4) regular emulsion	591:610	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	42	from	emulsion	473:480	arg1	emulsion					559:566	(3) stable emulsion	548:566	(3) stable emulsion (0.24-0.31% CNFs)	548:584	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	42	from	emulsion	473:480	arg1	CNFs					541:544	0.006-0.21% CNFs	529:544	0.006-0.21% CNFs	529:544	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	42	from	emulsion	473:480	arg1	absence					489:495	the absence	485:495	the absence of CNFs	485:503	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	42	from	emulsion	473:480	arg1	emulsion					519:526	(2) unstable emulsion	506:526	(2) unstable emulsion (0.006-0.21% CNFs)	506:545	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	42	from	emulsion	473:480	arg1	CNFs					580:583	0.24-0.31% CNFs	569:583	0.24-0.31% CNFs	569:583	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	43	theme	CNFs	500:503	arg1	emulsion					603:610	(4) regular emulsion	591:610	(4) regular emulsion	591:610	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	43	theme	CNFs	500:503	arg1	emulsion					559:566	(3) stable emulsion	548:566	(3) stable emulsion (0.24-0.31% CNFs)	548:584	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	43	theme	CNFs	500:503	arg1	CNFs					541:544	0.006-0.21% CNFs	529:544	0.006-0.21% CNFs	529:544	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	43	theme	CNFs	500:503	arg1	absence					489:495	the absence	485:495	the absence of CNFs	485:503	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	43	theme	CNFs	500:503	arg1	emulsion					519:526	(2) unstable emulsion	506:526	(2) unstable emulsion (0.006-0.21% CNFs)	506:545	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	43	theme	CNFs	500:503	arg1	CNFs					580:583	0.24-0.31% CNFs	569:583	0.24-0.31% CNFs	569:583	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	1	44	theme	%	254:254	arg1	SEO					272:274	SEO	272:274	SEO	272:274	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	1	44	theme	%	254:254	arg1	oil					267:269	0.5% sandalwood oil	251:269	0.5% sandalwood oil (SEO)	251:275	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	9	45	theme	fruit	1567:1571	arg1	commodities					1573:1583	fresh fruit commodities	1561:1583	fresh fruit commodities	1561:1583	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	6	46	theme	membrane	1117:1124	arg1	integrity					1126:1134	membrane integrity	1117:1134	membrane integrity	1117:1134	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	3	47	dep	emulsion	519:526	arg1	2					507:507	2	507:507	2	507:507	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	7	48	theme	visible	1284:1290	arg1	wavelengths					1298:1308	UV and visible light wavelengths	1277:1308	wavelengths	1298:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	9	49	contain	have	1510:1513	arg2	applications					1525:1536	potential applications	1515:1536	potential applications for active coating for fresh fruit commodities	1515:1583	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	9	49	contain	have	1510:1513	arg1	coating					1498:1504	Emulsion-CNF stabilized coating	1474:1504	Emulsion-CNF stabilized coating	1474:1504	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	5	50	theme	Antifungal	760:769	arg1	tests					771:775	Antifungal tests	760:775	Antifungal tests against Botrytis cinerea and Penicillium digitatum, between emulsion coating	760:852	Antifungal tests against Botrytis cinerea and Penicillium digitatum, between emulsion coating stabilized with CNFs (CS-SEOpick) and CS or CS-SEO was tested.					
34531497	4	51	theme	diameter	735:742	arg1	characteristics					708:722	the characteristics	704:722	the characteristics of droplet diameter and morphology	704:757	Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology.					
34531497	7	52	theme	UV	1277:1278	arg1	wavelengths					1298:1308	UV and visible light wavelengths	1277:1308	wavelengths	1298:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	0	53	theme	fresh	146:150	arg1	application					158:168	fresh fruit application	146:168	fresh fruit application	146:168	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	2	54	theme	Cellulose	278:286	arg1	CNFs					300:303	CNFs	300:303	CNFs	300:303	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	2	54	theme	Cellulose	278:286	arg1	nanofibers					288:297	Cellulose nanofibers	278:297	Cellulose nanofibers (CNFs)	278:304	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	2	54	theme	Cellulose	278:286	arg1	agent					332:336	a stabilizer agent	319:336	a stabilizer agent of oil-in-water Pickering emulsion	319:371	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	6	55	theme	antifungal	1023:1032	arg1	activity					1034:1041	CS-SEO antifungal activity	1016:1041	CS-SEO antifungal activity	1016:1041	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	1	56	theme	0.8	228:230	arg1	%					231:231	%	231:231	%	231:231	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	8	57	theme	force	1339:1343	arg1	microscopy					1345:1354	Atomic force microscopy	1332:1354	Atomic force microscopy	1332:1354	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	7	58	theme	SEO-loaded	1231:1240	arg1	CS					1242:1243	SEO-loaded CS	1231:1243	SEO-loaded CS	1231:1243	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	6	59	theme	CS-SEO	1016:1021	arg1	activity					1034:1041	CS-SEO antifungal activity	1016:1041	CS-SEO antifungal activity	1016:1041	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	7	60	theme	CS	1224:1225	arg1	strength					1322:1329	tensile strength	1314:1329	tensile strength	1314:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	60	theme	CS	1224:1225	arg1	properties					1210:1219	the functional properties	1195:1219	the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength	1195:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	60	theme	CS	1224:1225	arg1	transmission					1261:1272	light transmission	1255:1272	light transmission at UV and visible light wavelengths	1255:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	6	61	dep	assays	1086:1091	arg1	vitro					1097:1101	in vitro	1094:1101	in vitro	1094:1101	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	6	61	dep	assays	1086:1091	arg1	vivo					1107:1110	in vivo	1104:1110	in vivo	1104:1110	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	6	61	dep	assays	1086:1091	arg1	integrity					1126:1134	membrane integrity	1117:1134	membrane integrity	1117:1134	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	0	62	theme	coating	81:87	arg1	properties					24:33	properties	24:33	properties	24:33	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	0	62	theme	coating	81:87	arg1	features					11:18	Antifungal features	0:18	Antifungal features	0:18	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	8	63	theme	electron	1369:1376	arg1	microscopy					1378:1387	scanning electron microscopy	1360:1387	scanning electron microscopy	1360:1387	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	1	64	theme	novel	173:177	arg1	edible					189:194	A novel composite edible	171:194	A novel composite edible coating film	171:207	A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO).					
34531497	0	65	theme	cellulose	115:123	arg1	dosage					135:140	appropriate cellulose nanofiber dosage	103:140	appropriate cellulose nanofiber dosage for fresh fruit application	103:168	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	7	66	from	wavelengths	1298:1308	arg1	strength					1322:1329	tensile strength	1314:1329	tensile strength	1314:1329	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	7	66	from	wavelengths	1298:1308	arg1	transmission					1261:1272	light transmission	1255:1272	light transmission at UV and visible light wavelengths	1255:1308	The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength.					
34531497	4	67	theme	Confocal	645:652	arg1	microscopy					669:678	Confocal laser scanning microscopy	645:678	Confocal laser scanning microscopy	645:678	Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology.					
34531497	2	68	theme	Pickering	354:362	arg1	emulsion					364:371	oil-in-water Pickering emulsion	341:371	oil-in-water Pickering emulsion	341:371	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	5	69	theme	emulsion	837:844	arg1	coating					846:852	emulsion coating	837:852	emulsion coating	837:852	Antifungal tests against Botrytis cinerea and Penicillium digitatum, between emulsion coating stabilized with CNFs (CS-SEOpick) and CS or CS-SEO was tested.					
34531497	6	70	theme	CNFs	948:951	arg1	concentration					931:943	The effective concentration	917:943	The effective concentration of CNFs (0.24%)	917:959	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	8	71	theme	coating	1448:1454	arg1	formulation					1461:1471	each coating film formulation	1443:1471	each coating film formulation	1443:1471	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	3	72	theme	stable	552:557	arg1	emulsion					559:566	(3) stable emulsion	548:566	(3) stable emulsion (0.24-0.31% CNFs)	548:584	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	72	theme	stable	552:557	arg1	CNFs					580:583	0.24-0.31% CNFs	569:583	0.24-0.31% CNFs	569:583	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	5	73	dep	Botrytis	785:792	arg1	cinerea					794:800	cinerea	794:800	cinerea	794:800	Antifungal tests against Botrytis cinerea and Penicillium digitatum, between emulsion coating stabilized with CNFs (CS-SEOpick) and CS or CS-SEO was tested.					
34531497	0	74	theme	chitosan/sandalwood	38:56	arg1	coating					81:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	9	75	theme	stabilized	1487:1496	arg1	coating					1498:1504	Emulsion-CNF stabilized coating	1474:1504	Emulsion-CNF stabilized coating	1474:1504	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	8	76	theme	formulation	1461:1471	arg1	biocompatibility					1423:1438	the biocompatibility	1419:1438	the biocompatibility of each coating film formulation	1419:1471	Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation.					
34531497	3	77	theme	emulsion	426:433	arg1	stabilization					435:447	CNF level-dependent emulsion stabilization	406:447	CNF level-dependent emulsion stabilization	406:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	0	78	theme	Pickering	62:70	arg1	coating					81:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	chitosan/sandalwood oil Pickering emulsion coating	38:87	Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application.					
34531497	3	79	theme	%	578:578	arg1	emulsion					559:566	(3) stable emulsion	548:566	(3) stable emulsion (0.24-0.31% CNFs)	548:584	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	79	theme	%	578:578	arg1	CNFs					580:583	0.24-0.31% CNFs	569:583	0.24-0.31% CNFs	569:583	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	3	80	dep	emulsion	603:610	arg1	4					592:592	4	592:592	4	592:592	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	9	81	theme	potential	1515:1523	arg1	applications					1525:1536	potential applications	1515:1536	potential applications for active coating for fresh fruit commodities	1515:1583	Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.					
34531497	6	82	theme	CS	992:993	arg1	coating					995:1001	CS coating	992:1001	CS coating	992:1001	The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes).					
34531497	4	83	theme	scanning	660:667	arg1	microscopy					669:678	Confocal laser scanning microscopy	645:678	Confocal laser scanning microscopy	645:678	Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology.					
34531497	3	84	theme	CNF	406:408	arg1	stabilization					435:447	CNF level-dependent emulsion stabilization	406:447	CNF level-dependent emulsion stabilization	406:447	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
34531497	2	85	theme	stabilizer	321:330	arg1	nanofibers					288:297	Cellulose nanofibers	278:297	Cellulose nanofibers (CNFs)	278:304	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	2	85	theme	stabilizer	321:330	arg1	agent					332:336	a stabilizer agent	319:336	a stabilizer agent of oil-in-water Pickering emulsion	319:371	Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion.					
34531497	3	86	dep	emulsion	559:566	arg1	3					549:549	3	549:549	3	549:549	We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006-0.21% CNFs); (3) stable emulsion (0.24-0.31% CNFs); and (4) regular emulsion with the addition of surfactant.					
32045893	7	0	theme	marrow	1181:1186	arg1	microenvironment					1188:1203	the in vivo bone marrow microenvironment	1164:1203	the in vivo bone marrow microenvironment	1164:1203	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	2	1	theme	3D	343:344	arg1	scaffolds					346:354	3D scaffolds	343:354	3D scaffolds	343:354	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	3	2	theme	fibroin/carboxymethyl	653:673	arg1	GO/SF/CMCS					685:694	GO/SF/CMCS	685:694	GO/SF/CMCS	685:694	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	3	2	theme	fibroin/carboxymethyl	653:673	arg1	chitosan					675:682	graphene oxide/silk fibroin/carboxymethyl chitosan	633:682	graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS)	633:695	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	6	3	dep	culture	959:965	arg1	contrast					928:935	contrast	928:935	contrast	928:935	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	6	4	theme	reported	1073:1080	arg1	results					1091:1097	the previously reported clinical results	1058:1097	the previously reported clinical results	1058:1097	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	1	5	theme	drug	249:252	arg1	results					259:265	the drug test results	245:265	the drug test results	245:265	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	3	6	theme	chitosan	675:682	arg1	scaffolds					620:628	3D scaffolds	617:628	3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS)	617:695	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	0	7	dep	in	49:50	arg1	vitro					52:56	vitro	52:56	vitro	52:56	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	6	8	theme	drug	1016:1019	arg1	resistance					1021:1030	stronger drug resistance	1007:1030	stronger drug resistance	1007:1030	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	1	9	theme	test	254:257	arg1	results					259:265	the drug test results	245:265	the drug test results	245:265	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	0	10	theme	leukemia	103:110	arg1	drug					112:115	leukemia drug	103:115	leukemia drug	103:115	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	2	11	from	effect	441:446	arg1	cells					460:464	leukemia cells	451:464	leukemia cells	451:464	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	7	12	theme	3D	1122:1123	arg1	scaffolds					1136:1144	these 3D GO/SF/CMCS scaffolds	1116:1144	these 3D GO/SF/CMCS scaffolds	1116:1144	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	1	13	theme	results	259:265	arg1	%					240:240	95%	238:240	95% of the drug test results	238:265	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	1	13	theme	results	259:265	arg1	results					259:265	the drug test results	245:265	the drug test results	245:265	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	7	14	theme	drug	1253:1256	arg1	screening					1258:1266	preliminary drug screening	1241:1266	preliminary drug screening for clinical use	1241:1283	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	4	15	theme	better	758:763	arg1	ratio					774:778	a better swelling ratio	756:778	a better swelling ratio	756:778	Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO.					
32045893	1	16	theme	in	176:177	arg1	microenvironment					185:200	the in vitro microenvironment	172:200	the in vitro microenvironment for leukemia cells	172:219	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	7	17	theme	in	1168:1169	arg1	microenvironment					1188:1203	the in vivo bone marrow microenvironment	1164:1203	the in vivo bone marrow microenvironment	1164:1203	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	0	18	theme	in	49:50	arg1	microenvironment					65:80	in vitro marrow microenvironment	49:80	in vitro marrow microenvironment	49:80	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	0	19	theme	porous	18:23	arg1	scaffolds					35:43	Three-dimensional porous composite scaffolds	0:43	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment	0:80	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	7	20	theme	preliminary	1241:1251	arg1	screening					1258:1266	preliminary drug screening	1241:1266	preliminary drug screening for clinical use	1241:1283	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	5	21	theme	scaffolds	847:855	arg1	hydrophilicity					825:838	The improved hydrophilicity	812:838	The improved hydrophilicity of the scaffolds	812:855	The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells.					
32045893	0	22	theme	Three-dimensional	0:16	arg1	scaffolds					35:43	Three-dimensional porous composite scaffolds	0:43	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment	0:80	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	6	23	from	cells	977:981	arg1	culture					994:1000	this 3D culture	986:1000	this 3D culture	986:1000	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	5	24	theme	improved	816:823	arg1	hydrophilicity					825:838	The improved hydrophilicity	812:838	The improved hydrophilicity of the scaffolds	812:855	The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells.					
32045893	6	25	theme	2D	956:957	arg1	culture					959:965	the traditional 2D culture	940:965	the traditional 2D culture	940:965	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	2	26	theme	clinical	524:531	arg1	use					533:535	clinical use	524:535	clinical use	524:535	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	5	27	theme	cells	918:922	arg1	adhesion					879:886	adhesion	879:886	adhesion	879:886	The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells.					
32045893	5	27	theme	cells	918:922	arg1	proliferation					892:904	proliferation	892:904	proliferation	892:904	The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells.					
32045893	4	28	theme	GO	808:809	arg1	embedding					795:803	the embedding	791:803	the embedding of GO	791:809	Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO.					
32045893	1	29	theme	leukemia	206:213	arg1	cells					215:219	leukemia cells	206:219	leukemia cells	206:219	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	0	30	theme	composite	25:33	arg1	scaffolds					35:43	Three-dimensional porous composite scaffolds	0:43	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment	0:80	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	6	31	theme	traditional	944:954	arg1	culture					959:965	the traditional 2D culture	940:965	the traditional 2D culture	940:965	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	7	32	theme	promising	1218:1226	arg1	platform					1228:1235	a promising platform	1216:1235	a promising platform for preliminary drug screening for clinical use	1216:1283	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	2	33	theme	leukemia	451:458	arg1	cells					460:464	leukemia cells	451:464	leukemia cells	451:464	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	2	34	theme	2D	331:332	arg1	culture					334:340	this 2D culture	326:340	this 2D culture	326:340	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	2	35	theme	drugs	514:518	arg1	screening					501:509	the preliminary screening	485:509	the preliminary screening of drugs for clinical use	485:535	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	3	36	theme	3D	617:618	arg1	scaffolds					620:628	3D scaffolds	617:628	3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS)	617:695	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	7	37	theme	clinical	1272:1279	arg1	use					1281:1283	clinical use	1272:1283	clinical use	1272:1283	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	2	38	theme	marrow	385:390	arg1	microenvironment					392:407	the bone marrow microenvironment	376:407	the bone marrow microenvironment	376:407	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	7	39	theme	GO/SF/CMCS	1125:1134	arg1	scaffolds					1136:1144	these 3D GO/SF/CMCS scaffolds	1116:1144	these 3D GO/SF/CMCS scaffolds	1116:1144	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	1	40	theme	subsequent	290:299	arg1	results					310:316	the subsequent clinical results	286:316	the subsequent clinical results	286:316	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	3	41	theme	freeze-drying	560:572	arg1	method					574:579	the freeze-drying method	556:579	the freeze-drying method	556:579	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	2	42	theme	bone	380:383	arg1	microenvironment					392:407	the bone marrow microenvironment	376:407	the bone marrow microenvironment	376:407	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	0	43	theme	marrow	58:63	arg1	microenvironment					65:80	in vitro marrow microenvironment	49:80	in vitro marrow microenvironment	49:80	Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.					
32045893	1	44	theme	clinical	301:308	arg1	results					310:316	the subsequent clinical results	286:316	the subsequent clinical results	286:316	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	6	45	with	consistent	1042:1051	arg1	results					1091:1097	the previously reported clinical results	1058:1097	the previously reported clinical results	1058:1097	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	1	46	dep	in	176:177	arg1	vitro					179:183	vitro	179:183	vitro	179:183	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	1	47	theme	traditional	122:132	arg1	medium					145:150	The traditional 2D culture medium	118:150	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells	118:219	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	2	48	theme	drug	436:439	arg1	effect					441:446	the drug effect	432:446	the drug effect on leukemia cells	432:464	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32045893	7	49	dep	in	1168:1169	arg1	vivo					1171:1174	vivo	1171:1174	vivo	1171:1174	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	3	50	theme	graphene	633:640	arg1	GO/SF/CMCS					685:694	GO/SF/CMCS	685:694	GO/SF/CMCS	685:694	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	3	50	theme	graphene	633:640	arg1	chitosan					675:682	graphene oxide/silk fibroin/carboxymethyl chitosan	633:682	graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS)	633:695	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	7	51	theme	bone	1176:1179	arg1	microenvironment					1188:1203	the in vivo bone marrow microenvironment	1164:1203	the in vivo bone marrow microenvironment	1164:1203	It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.					
32045893	1	52	theme	2D	134:135	arg1	medium					145:150	The traditional 2D culture medium	118:150	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells	118:219	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	3	53	theme	oxide/silk	642:651	arg1	GO/SF/CMCS					685:694	GO/SF/CMCS	685:694	GO/SF/CMCS	685:694	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	3	53	theme	oxide/silk	642:651	arg1	chitosan					675:682	graphene oxide/silk fibroin/carboxymethyl chitosan	633:682	graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS)	633:695	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	1	54	theme	culture	137:143	arg1	medium					145:150	The traditional 2D culture medium	118:150	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells	118:219	The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results.					
32045893	4	55	theme	3D	735:736	arg1	scaffolds					738:746	these 3D scaffolds	729:746	these 3D scaffolds	729:746	Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO.					
32045893	3	56	theme	scaffolds	620:628	arg1	fabrication					602:612	the fabrication	598:612	the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS)	598:695	For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS).					
32045893	4	57	theme	swelling	765:772	arg1	ratio					774:778	a better swelling ratio	756:778	a better swelling ratio	756:778	Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO.					
32045893	4	58	theme	Experimental	698:709	arg1	results					711:717	Experimental results	698:717	Experimental results	698:717	Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO.					
32045893	5	59	theme	leukemia	909:916	arg1	cells					918:922	leukemia cells	909:922	leukemia cells	909:922	The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells.					
32045893	6	60	theme	stronger	1007:1014	arg1	resistance					1021:1030	stronger drug resistance	1007:1030	stronger drug resistance	1007:1030	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	6	61	theme	clinical	1082:1089	arg1	results					1091:1097	the previously reported clinical results	1058:1097	the previously reported clinical results	1058:1097	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	6	62	theme	3D	991:992	arg1	culture					994:1000	this 3D culture	986:1000	this 3D culture	986:1000	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	6	63	theme	leukemia	968:975	arg1	cells					977:981	leukemia cells	968:981	leukemia cells in this 3D culture	968:1000	In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results.					
32045893	5	64	theme	promoted	870:877	arg1	adhesion					879:886	adhesion	879:886	adhesion	879:886	The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells.					
32045893	2	65	theme	preliminary	489:499	arg1	screening					501:509	the preliminary screening	485:509	the preliminary screening of drugs for clinical use	485:535	Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use.					
32447128	4	0	theme	good	469:472	arg1	stability					482:490	a good storage stability	467:490	a good storage stability (7 months 40% residual activity)	467:523	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	2	1	theme	support	244:250	arg1	precursors					252:261	the support precursors	240:261	the support precursors	240:261	The innovative method proposed is based on the use of Ca(II) as a linker of the support precursors.					
32447128	5	2	theme	optimum	666:672	arg1	temperature					685:695	optimum incubation temperature	666:695	optimum incubation temperature of 50 °C	666:704	Furthermore, the biocatalyst showed optimum pH 3 and optimum incubation temperature of 50 °C.					
32447128	4	3	dep	stability	482:490	arg1	months					495:500	7 months	493:500	7 months 40% residual activity	493:522	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	5	4	theme	incubation	674:683	arg1	temperature					685:695	optimum incubation temperature	666:695	optimum incubation temperature of 50 °C	666:704	Furthermore, the biocatalyst showed optimum pH 3 and optimum incubation temperature of 50 °C.					
32447128	3	5	theme	reactive	389:396	arg1	aldehydes					379:387	aldehydes	379:387	aldehydes reactive towards the NH2 groups of chitosan	379:431	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	3	6	theme	glycosidic	311:320	arg1	part					322:325	the glycosidic part	307:325	the glycosidic part of enzyme	307:335	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	4	7	theme	immobilized	438:448	arg1	laccase					450:456	The immobilized laccase	434:456	The immobilized laccase	434:456	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	1	8	theme	study	75:79	arg1	aim					63:65	The aim	59:65	The aim of this study	59:79	The aim of this study was to optimize the laccase immobilization on a silica/chitosan composite support.					
32447128	4	9	dep	months	495:500	arg1	activity					515:522	40% residual activity	502:522	7 months 40% residual activity	493:522	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	0	10	theme	Silica-chitosan	0:14	arg1	hybrid					16:21	Silica-chitosan hybrid	0:21	Silica-chitosan hybrid	0:21	Silica-chitosan hybrid support for laccase immobilization.					
32447128	4	11	theme	40	502:503	arg1	%					504:504	%	504:504	%	504:504	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	3	12	theme	enzyme	330:335	arg1	part					322:325	the glycosidic part	307:325	the glycosidic part of enzyme	307:335	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	1	13	from	immobilization	109:122	arg1	support					155:161	a silica/chitosan composite support	127:161	a silica/chitosan composite support	127:161	The aim of this study was to optimize the laccase immobilization on a silica/chitosan composite support.					
32447128	4	14	theme	residual	506:513	arg1	activity					515:522	40% residual activity	502:522	7 months 40% residual activity	493:522	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	5	15	theme	optimum	649:655	arg1	pH					657:658	optimum pH 3	649:660	optimum pH 3	649:660	Furthermore, the biocatalyst showed optimum pH 3 and optimum incubation temperature of 50 °C.					
32447128	2	16	theme	precursors	252:261	arg1	linker					230:235	a linker	228:235	a linker of the support precursors	228:261	The innovative method proposed is based on the use of Ca(II) as a linker of the support precursors.					
32447128	4	17	theme	%	504:504	arg1	activity					515:522	40% residual activity	502:522	7 months 40% residual activity	493:522	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	0	18	theme	laccase	35:41	arg1	immobilization					43:56	laccase immobilization	35:56	laccase immobilization	35:56	Silica-chitosan hybrid support for laccase immobilization.					
32447128	2	19	theme	innovative	168:177	arg1	method					179:184	The innovative method	164:184	The innovative method proposed	164:193	The innovative method proposed is based on the use of Ca(II) as a linker of the support precursors.					
32447128	2	20	theme	Ca	218:219	arg1	use					211:213	the use	207:213	the use of Ca(II) as a linker of the support precursors	207:261	The innovative method proposed is based on the use of Ca(II) as a linker of the support precursors.					
32447128	3	21	theme	NH2	410:412	arg1	chitosan					424:431	chitosan	424:431	chitosan	424:431	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	3	21	theme	NH2	410:412	arg1	groups					414:419	the NH2 groups	406:419	the NH2 groups of chitosan	406:431	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	4	22	theme	storage	474:480	arg1	stability					482:490	a good storage stability	467:490	a good storage stability (7 months 40% residual activity)	467:523	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	1	23	theme	laccase	101:107	arg1	immobilization					109:122	the laccase immobilization	97:122	the laccase immobilization on a silica/chitosan composite support	97:161	The aim of this study was to optimize the laccase immobilization on a silica/chitosan composite support.					
32447128	4	24	dep	substrate	558:566	arg1	Kmapp					569:573	Kmapp	569:573	Kmapp	569:573	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	4	24	dep	substrate	558:566	arg1	Vmaxapp = 0.0034 μmol/min					585:609	Vmaxapp = 0.0034 μmol/min	585:609	Vmaxapp = 0.0034 μmol/min	585:609	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	3	25	theme	chitosan	424:431	arg1	chitosan					424:431	chitosan	424:431	chitosan	424:431	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	3	25	theme	chitosan	424:431	arg1	groups					414:419	the NH2 groups	406:419	the NH2 groups of chitosan	406:431	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	4	26	theme	affinity	536:543	arg1	substrate					558:566	a good affinity towards ABTS substrate	529:566	a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min)	529:610	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	4	27	theme	good	531:534	arg1	substrate					558:566	a good affinity towards ABTS substrate	529:566	a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min)	529:610	The immobilized laccase presents a good storage stability (7 months 40% residual activity) and a good affinity towards ABTS substrate (Kmapp 0.008 mM, Vmaxapp = 0.0034 μmol/min).					
32447128	3	28	theme	laccase	284:290	arg1	immobilization					292:305	the laccase immobilization	280:305	the laccase immobilization	280:305	In addition for the laccase immobilization the glycosidic part of enzyme is oxidized by periodate in order to form aldehydes reactive towards the NH2 groups of chitosan.					
32447128	1	29	theme	silica/chitosan	129:143	arg1	support					155:161	a silica/chitosan composite support	127:161	a silica/chitosan composite support	127:161	The aim of this study was to optimize the laccase immobilization on a silica/chitosan composite support.					
32447128	5	30	theme	50 °C	700:704	arg1	temperature					685:695	optimum incubation temperature	666:695	optimum incubation temperature of 50 °C	666:704	Furthermore, the biocatalyst showed optimum pH 3 and optimum incubation temperature of 50 °C.					
32447128	5	30	theme	50 °C	700:704	arg1	pH					657:658	optimum pH 3	649:660	optimum pH 3	649:660	Furthermore, the biocatalyst showed optimum pH 3 and optimum incubation temperature of 50 °C.					
32447128	1	31	theme	composite	145:153	arg1	support					155:161	a silica/chitosan composite support	127:161	a silica/chitosan composite support	127:161	The aim of this study was to optimize the laccase immobilization on a silica/chitosan composite support.					
34082377	0	0	theme	effective	97:105	arg1	agent					121:125	A reusable and effective antibacterial agent	82:125	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.	0:167	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	6	1	theme	new	1019:1021	arg1	method					1041:1046	a new effective control method	1017:1046	a new effective control method for A. acidoterrestris in food field	1017:1083	The aim of this work is to develop a new effective control method for A. acidoterrestris in food field.					
34082377	5	2	theme	serious	897:903	arg1	leakage					905:911	serious leakage	897:911	serious leakage of intracellular protein	897:936	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	0	3	theme	antibacterial	107:119	arg1	agent					121:125	A reusable and effective antibacterial agent	82:125	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.	0:167	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	2	4	from	Base	251:254	arg1	property					264:271	this property	259:271	this property	259:271	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	1	5	theme	cationic	199:206	arg1	Epsilon-polylysine					169:186	Epsilon-polylysine	169:186	Epsilon-polylysine (EPL)	169:192	Epsilon-polylysine (EPL) is a cationic polymer with broad antibacterial activity.					
34082377	1	5	theme	cationic	199:206	arg1	polymer					208:214	a cationic polymer	197:214	a cationic polymer with broad antibacterial activity	197:248	Epsilon-polylysine (EPL) is a cationic polymer with broad antibacterial activity.					
34082377	5	6	theme	intracellular	916:928	arg1	protein					930:936	intracellular protein	916:936	intracellular protein	916:936	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	6	7	theme	work	998:1001	arg1	aim					986:988	The aim	982:988	The aim of this work	982:1001	The aim of this work is to develop a new effective control method for A. acidoterrestris in food field.					
34082377	3	8	theme	successful	453:462	arg1	synthesis					464:472	the successful synthesis	449:472	the successful synthesis of composites	449:486	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	3	9	theme	characterization	489:504	arg1	VSM					552:554	VSM	552:554	VSM	552:554	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	3	9	theme	characterization	489:504	arg1	FT-IR					532:536	FT-IR	532:536	FT-IR	532:536	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	3	9	theme	characterization	489:504	arg1	TEM					539:541	TEM	539:541	TEM	539:541	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	3	9	theme	characterization	489:504	arg1	techniques					506:515	characterization techniques	489:515	characterization techniques including XRD, FT-IR, TEM, XPS and VSM	489:554	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	3	9	theme	characterization	489:504	arg1	XRD					527:529	XRD	527:529	XRD	527:529	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	3	9	theme	characterization	489:504	arg1	XPS					544:546	XPS	544:546	XPS	544:546	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	5	10	theme	protein	930:936	arg1	damage					865:870	irreversible damage	852:870	irreversible damage to the cell membrane	852:891	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	5	10	theme	protein	930:936	arg1	leakage					905:911	serious leakage	897:911	serious leakage of intracellular protein	897:936	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	0	11	theme	One-pot	0:6	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.	0:167	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	3	12	theme	composites	477:486	arg1	synthesis					464:472	the successful synthesis	449:472	the successful synthesis of composites	449:486	To demonstrate the successful synthesis of composites, characterization techniques including XRD, FT-IR, TEM, XPS and VSM were employed.					
34082377	0	13	theme	magnetic	21:28	arg1	composites					70:79	magnetic self-assembled carrageenan-ε-polylysine composites	21:79	magnetic self-assembled carrageenan-ε-polylysine composites	21:79	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	6	14	theme	food	1074:1077	arg1	field					1079:1083	food field	1074:1083	food field	1074:1083	The aim of this work is to develop a new effective control method for A. acidoterrestris in food field.					
34082377	1	15	theme	antibacterial	227:239	arg1	activity					241:248	broad antibacterial activity	221:248	broad antibacterial activity	221:248	Epsilon-polylysine (EPL) is a cationic polymer with broad antibacterial activity.					
34082377	4	16	theme	antibacterial	615:627	arg1	activity					629:636	effective antibacterial activity	605:636	effective antibacterial activity against Alicyclobacillus acidoterrestris	605:677	The prepared composites exhibited effective antibacterial activity against Alicyclobacillus acidoterrestris with the minimum inhibitory concentration of 10.0 mg mL-1.					
34082377	0	17	theme	carrageenan-ε-polylysine	45:68	arg1	composites					70:79	magnetic self-assembled carrageenan-ε-polylysine composites	21:79	magnetic self-assembled carrageenan-ε-polylysine composites	21:79	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	4	18	theme	effective	605:613	arg1	activity					629:636	effective antibacterial activity	605:636	effective antibacterial activity against Alicyclobacillus acidoterrestris	605:677	The prepared composites exhibited effective antibacterial activity against Alicyclobacillus acidoterrestris with the minimum inhibitory concentration of 10.0 mg mL-1.					
34082377	0	19	theme	Alicyclobacillus	135:150	arg1	acidoterrestris					152:166	Alicyclobacillus acidoterrestris	135:166	Alicyclobacillus acidoterrestris	135:166	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	2	20	theme	Fe3O4-CG-EPL	409:420	arg1	composites					422:431	the magnetic Fe3O4-CG-EPL composites	396:431	the magnetic Fe3O4-CG-EPL composites	396:431	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	0	21	theme	self-assembled	30:43	arg1	composites					70:79	magnetic self-assembled carrageenan-ε-polylysine composites	21:79	magnetic self-assembled carrageenan-ε-polylysine composites	21:79	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	5	22	theme	Fe3O4-CG-EPL	832:843	arg1	presence					820:827	the presence	816:827	the presence of Fe3O4-CG-EPL	816:843	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	5	23	theme	irreversible	852:863	arg1	damage					865:870	irreversible damage	852:870	irreversible damage to the cell membrane	852:891	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	4	24	theme	10.0 mg mL-1	724:735	arg1	concentration					707:719	the minimum inhibitory concentration	684:719	the minimum inhibitory concentration of 10.0 mg mL-1	684:735	The prepared composites exhibited effective antibacterial activity against Alicyclobacillus acidoterrestris with the minimum inhibitory concentration of 10.0 mg mL-1.					
34082377	5	25	theme	antibacterial	774:786	arg1	mechanism					788:796	the antibacterial mechanism	770:796	the antibacterial mechanism	770:796	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	1	26	theme	broad	221:225	arg1	activity					241:248	broad antibacterial activity	221:248	broad antibacterial activity	221:248	Epsilon-polylysine (EPL) is a cationic polymer with broad antibacterial activity.					
34082377	0	27	theme	composites	70:79	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.	0:167	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	4	28	theme	prepared	575:582	arg1	composites					584:593	The prepared composites	571:593	The prepared composites	571:593	The prepared composites exhibited effective antibacterial activity against Alicyclobacillus acidoterrestris with the minimum inhibitory concentration of 10.0 mg mL-1.					
34082377	2	29	theme	coprecipitation	359:373	arg1	process					375:381	the one-pot coprecipitation process	347:381	the one-pot coprecipitation process	347:381	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	6	30	theme	control	1033:1039	arg1	method					1041:1046	a new effective control method	1017:1046	a new effective control method for A. acidoterrestris in food field	1017:1083	The aim of this work is to develop a new effective control method for A. acidoterrestris in food field.					
34082377	2	31	theme	one-pot	351:357	arg1	process					375:381	the one-pot coprecipitation process	347:381	the one-pot coprecipitation process	347:381	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	1	32	with	polymer	208:214	arg1	activity					241:248	broad antibacterial activity	221:248	broad antibacterial activity	221:248	Epsilon-polylysine (EPL) is a cationic polymer with broad antibacterial activity.					
34082377	0	33	theme	reusable	84:91	arg1	agent					121:125	A reusable and effective antibacterial agent	82:125	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.	0:167	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	5	34	theme	bacteria	972:979	arg1	inactivation					956:967	the inactivation	952:967	the inactivation of bacteria	952:979	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	0	35	dep	synthesis	8:16	arg1	agent					121:125	A reusable and effective antibacterial agent	82:125	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.	0:167	One-pot synthesis of magnetic self-assembled carrageenan-ε-polylysine composites: A reusable and effective antibacterial agent against Alicyclobacillus acidoterrestris.					
34082377	2	36	theme	magnetic	400:407	arg1	composites					422:431	the magnetic Fe3O4-CG-EPL composites	396:431	the magnetic Fe3O4-CG-EPL composites	396:431	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	4	37	theme	inhibitory	696:705	arg1	concentration					707:719	the minimum inhibitory concentration	684:719	the minimum inhibitory concentration of 10.0 mg mL-1	684:735	The prepared composites exhibited effective antibacterial activity against Alicyclobacillus acidoterrestris with the minimum inhibitory concentration of 10.0 mg mL-1.					
34082377	5	38	theme	cell	879:882	arg1	membrane					884:891	the cell membrane	875:891	the cell membrane	875:891	Investigations into deciphering the antibacterial mechanism demonstrated that the presence of Fe3O4-CG-EPL caused irreversible damage to the cell membrane and serious leakage of intracellular protein, resulting in the inactivation of bacteria.					
34082377	6	39	theme	effective	1023:1031	arg1	method					1041:1046	a new effective control method	1017:1046	a new effective control method for A. acidoterrestris in food field	1017:1083	The aim of this work is to develop a new effective control method for A. acidoterrestris in food field.					
34082377	2	40	theme	anionic	274:280	arg1	carrageenan					282:292	anionic carrageenan	274:292	anionic carrageenan (CG)	274:297	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	2	40	theme	anionic	274:280	arg1	Base					251:254	Base	251:254	Base on this property	251:271	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	2	40	theme	anionic	274:280	arg1	CG					295:296	CG	295:296	CG	295:296	Base on this property, anionic carrageenan (CG) was self-assembled with EPL and introduced into the one-pot coprecipitation process to fabricate the magnetic Fe3O4-CG-EPL composites.					
34082377	4	41	theme	minimum	688:694	arg1	concentration					707:719	the minimum inhibitory concentration	684:719	the minimum inhibitory concentration of 10.0 mg mL-1	684:735	The prepared composites exhibited effective antibacterial activity against Alicyclobacillus acidoterrestris with the minimum inhibitory concentration of 10.0 mg mL-1.					
33987106	8	0	theme	tail	1095:1098	arg1	suspension					1100:1109	tail suspension	1095:1109	tail suspension test	1095:1114	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	4	1	theme	CAP	484:486	arg1	effects					465:471	the effects	461:471	the effects of dietary CAP on gut microbes	461:502	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	1	2	from	ingredient	169:178	arg1	pepper					189:194	chili pepper	183:194	chili pepper	183:194	Capsaicin (CAP) is an active ingredient in chili pepper that is frequently consumed.					
33987106	8	3	theme	suspension	1100:1109	arg1	test					1111:1114	tail suspension test	1095:1114	tail suspension test	1095:1114	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	3	theme	suspension	1100:1109	arg1	test					1067:1070	sucrose preference test	1048:1070	sucrose preference test	1048:1070	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	11	4	theme	Correlation	1478:1488	arg1	analysis					1490:1497	Correlation analysis	1478:1497	Correlation analysis	1478:1497	Correlation analysis revealed changes in microbiota composition that was closely related to depressive behavior, 5-HT and TNF-α levels.					
33987106	0	5	theme	Depression-Like	76:90	arg1	Behavior					92:99	Depression-Like Behavior	76:99	Depression-Like Behavior Induced by Lipopolysaccharide in Mice	76:137	Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice.					
33987106	8	6	with	mice	1170:1173	arg1	behaviors					1196:1204	depression-like behaviors	1180:1204	depression-like behaviors	1180:1204	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	10	7	theme	microorganisms	1389:1402	arg1	abundance					1360:1368	relative abundance	1351:1368	relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira	1351:1475	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	5	8	theme	C57BL/6J	580:587	arg1	mice					594:597	C57BL/6J male mice	580:597	C57BL/6J male mice (four weeks old)	580:614	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	3	9	theme	antidepressant	347:360	arg1	effects					362:368	antidepressant effects	347:368	antidepressant effects	347:368	However, its mechanism of antidepressant effects is still unclear.					
33987106	4	10	theme	gut	491:493	arg1	microbes					495:502	gut microbes	491:502	gut microbes	491:502	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	5	11	theme	specific	627:634	arg1	feed					636:639	specific feed	627:639	specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP)	627:701	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	5	12	theme	male	589:592	arg1	mice					594:597	C57BL/6J male mice	580:597	C57BL/6J male mice (four weeks old)	580:614	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	2	13	theme	potential	284:292	arg1	effects					294:300	potential effects	284:300	potential effects	284:300	It exerts various pharmacological activities, and also has potential effects on mental illness.					
33987106	3	14	theme	effects	362:368	arg1	mechanism					334:342	its mechanism	330:342	its mechanism of antidepressant effects	330:368	However, its mechanism of antidepressant effects is still unclear.					
33987106	9	15	theme	abnormal	1238:1245	arg1	changes					1247:1253	abnormal changes	1238:1253	abnormal changes in depression-related microbiota	1238:1286	In addition, CAP could recover abnormal changes in depression-related microbiota.					
33987106	10	16	theme	certain	1373:1379	arg1	microorganisms					1389:1402	certain pivotal microorganisms	1373:1402	certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira	1373:1475	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	4	17	theme	emerging	401:408	arg1	perspective					410:420	the emerging perspective	397:420	the emerging perspective of the gut-brain axis	397:442	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	11	18	from	changes	1508:1514	arg1	composition					1530:1540	microbiota composition	1519:1540	microbiota composition	1519:1540	Correlation analysis revealed changes in microbiota composition that was closely related to depressive behavior, 5-HT and TNF-α levels.					
33987106	8	19	theme	dietary	1001:1007	arg1	CAP					1009:1011	dietary CAP	1001:1011	dietary CAP	1001:1011	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	7	20	theme	gut	907:909	arg1	microbiota					911:920	gut microbiota	907:920	gut microbiota	907:920	Behavioral indicators and serum parameters were measured, and gut microbiota were identified by sequencing analysis of the 16S gene.					
33987106	1	21	theme	active	162:167	arg1	Capsaicin					140:148	Capsaicin	140:148	Capsaicin (CAP)	140:154	Capsaicin (CAP) is an active ingredient in chili pepper that is frequently consumed.					
33987106	1	21	theme	active	162:167	arg1	ingredient					169:178	an active ingredient	159:178	an active ingredient in chili pepper that is frequently consumed	159:222	Capsaicin (CAP) is an active ingredient in chili pepper that is frequently consumed.					
33987106	4	22	theme	dietary	476:482	arg1	CAP					484:486	dietary CAP	476:486	dietary CAP	476:486	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	12	23	theme	depression	1754:1763	arg1	prevention					1740:1749	the prevention	1736:1749	the prevention of depression	1736:1763	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	12	24	theme	microbiota	1700:1709	arg1	number					1686:1691	number	1686:1691	number	1686:1691	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	12	24	theme	microbiota	1700:1709	arg1	structure					1672:1680	structure	1672:1680	structure	1672:1680	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	5	25	dep	feed	636:639	arg1	CAP					698:700	0.005% CAP	691:700	0.005% CAP	691:700	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	5	25	dep	feed	636:639	arg1	chow					662:665	standard laboratory chow	642:665	standard laboratory chow	642:665	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	5	25	dep	feed	636:639	arg1	chow					681:684	laboratory chow	670:684	laboratory chow	670:684	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	12	26	theme	dietary	1643:1649	arg1	CAP					1651:1653	dietary CAP	1643:1653	dietary CAP	1643:1653	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	8	27	theme	LPS-induced	1158:1168	arg1	mice					1170:1173	LPS-induced mice	1158:1173	LPS-induced mice with depression-like behaviors	1158:1204	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	10	28	dep	Allobaculum	1435:1445	arg1	Prevotella					1423:1432	Prevotella	1423:1432	Prevotella	1423:1432	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	9	29	theme	depression-related	1258:1275	arg1	microbiota					1277:1286	depression-related microbiota	1258:1286	depression-related microbiota	1258:1286	In addition, CAP could recover abnormal changes in depression-related microbiota.					
33987106	4	30	from	effects	465:471	arg1	microbes					495:502	gut microbes	491:502	gut microbes	491:502	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	6	31	theme	last	728:731	arg1	days					738:741	the last five days	724:741	the last five days	724:741	During the last five days, LPS (0.052/0.104/0.208/0.415/0.83 mg/kg, 5-day) was injected intraperitoneally to induce depression.					
33987106	9	32	from	changes	1247:1253	arg1	microbiota					1277:1286	depression-related microbiota	1258:1286	depression-related microbiota	1258:1286	In addition, CAP could recover abnormal changes in depression-related microbiota.					
33987106	8	33	theme	preference	1056:1065	arg1	test					1067:1070	sucrose preference test	1048:1070	sucrose preference test	1048:1070	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	33	theme	preference	1056:1065	arg1	test					1089:1092	forced swimming test	1073:1092	forced swimming test	1073:1092	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	33	theme	preference	1056:1065	arg1	test					1111:1114	tail suspension test	1095:1114	tail suspension test	1095:1114	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	34	theme	depression-like	1180:1194	arg1	behaviors					1196:1204	depression-like behaviors	1180:1204	depression-like behaviors	1180:1204	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	0	35	theme	Gut	0:2	arg1	Microbiota					4:13	Gut Microbiota	0:13	Gut Microbiota	0:13	Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice.					
33987106	8	36	theme	mice	1170:1173	arg1	serum					1149:1153	serum	1149:1153	serum of LPS-induced mice with depression-like behaviors	1149:1204	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	10	37	theme	relative	1351:1358	arg1	abundance					1360:1368	relative abundance	1351:1368	relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira	1351:1475	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	2	38	theme	mental	305:310	arg1	illness					312:318	mental illness	305:318	mental illness	305:318	It exerts various pharmacological activities, and also has potential effects on mental illness.					
33987106	11	39	theme	TNF-α	1600:1604	arg1	levels					1606:1611	TNF-α levels	1600:1611	TNF-α levels	1600:1611	Correlation analysis revealed changes in microbiota composition that was closely related to depressive behavior, 5-HT and TNF-α levels.					
33987106	1	40	theme	chili	183:187	arg1	pepper					189:194	chili pepper	183:194	chili pepper	183:194	Capsaicin (CAP) is an active ingredient in chili pepper that is frequently consumed.					
33987106	11	41	theme	microbiota	1519:1528	arg1	composition					1530:1540	microbiota composition	1519:1540	microbiota composition	1519:1540	Correlation analysis revealed changes in microbiota composition that was closely related to depressive behavior, 5-HT and TNF-α levels.					
33987106	12	42	dep	structure	1672:1680	arg1	the					1668:1670	the	1668:1670	the	1668:1670	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	4	43	with	mice	507:510	arg1	behaviors					533:541	depression-like behaviors	517:541	depression-like behaviors induced by lipopolysaccharide (LPS)	517:577	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	12	44	theme	major	1722:1726	arg1	role					1728:1731	a major role	1720:1731	a major role	1720:1731	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	8	45	theme	5-HT	1131:1134	arg1	levels					1121:1126	levels	1121:1126	levels of 5-HT and TNF-α	1121:1144	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	45	theme	5-HT	1131:1134	arg1	behavior					1038:1045	depressive-like behavior	1022:1045	depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test)	1022:1115	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	2	46	contain	has	280:282	arg1	It					225:226	It	225:226	It	225:226	It exerts various pharmacological activities, and also has potential effects on mental illness.					
33987106	2	46	contain	has	280:282	arg2	effects					294:300	potential effects	284:300	potential effects	284:300	It exerts various pharmacological activities, and also has potential effects on mental illness.					
33987106	5	47	theme	laboratory	670:679	arg1	chow					681:684	laboratory chow	670:684	laboratory chow	670:684	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	12	48	theme	gut	1696:1698	arg1	microbiota					1700:1709	gut microbiota	1696:1709	gut microbiota	1696:1709	These results suggested that dietary CAP can regulate the structure and number of gut microbiota and play a major role in the prevention of depression.					
33987106	8	49	theme	sucrose	1048:1054	arg1	test					1067:1070	sucrose preference test	1048:1070	sucrose preference test	1048:1070	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	49	theme	sucrose	1048:1054	arg1	test					1089:1092	forced swimming test	1073:1092	forced swimming test	1073:1092	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	49	theme	sucrose	1048:1054	arg1	test					1111:1114	tail suspension test	1095:1114	tail suspension test	1095:1114	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	4	50	theme	axis	439:442	arg1	perspective					410:420	the emerging perspective	397:420	the emerging perspective of the gut-brain axis	397:442	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	8	51	theme	depressive-like	1022:1036	arg1	behavior					1038:1045	depressive-like behavior	1022:1045	depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test)	1022:1115	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	6	52	dep	LPS	744:746	arg1	5-day					785:789	5-day	785:789	5-day	785:789	During the last five days, LPS (0.052/0.104/0.208/0.415/0.83 mg/kg, 5-day) was injected intraperitoneally to induce depression.					
33987106	6	52	dep	LPS	744:746	arg1	mg/kg					778:782	0.052/0.104/0.208/0.415/0.83 mg/kg	749:782	0.052/0.104/0.208/0.415/0.83 mg/kg	749:782	During the last five days, LPS (0.052/0.104/0.208/0.415/0.83 mg/kg, 5-day) was injected intraperitoneally to induce depression.					
33987106	0	53	theme	Preventive	28:37	arg1	Effects					39:45	the Preventive Effects	24:45	the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice	24:137	Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice.					
33987106	8	54	theme	TNF-α	1140:1144	arg1	levels					1121:1126	levels	1121:1126	levels of 5-HT and TNF-α	1121:1144	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	54	theme	TNF-α	1140:1144	arg1	behavior					1038:1045	depressive-like behavior	1022:1045	depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test)	1022:1115	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	7	55	theme	16S	968:970	arg1	gene					972:975	the 16S gene	964:975	the 16S gene	964:975	Behavioral indicators and serum parameters were measured, and gut microbiota were identified by sequencing analysis of the 16S gene.					
33987106	0	56	theme	Dietary	50:56	arg1	Capsaicin					58:66	Dietary Capsaicin	50:66	Dietary Capsaicin	50:66	Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice.					
33987106	5	57	theme	0.005	691:695	arg1	%					696:696	%	696:696	%	696:696	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	8	58	theme	swimming	1080:1087	arg1	test					1067:1070	sucrose preference test	1048:1070	sucrose preference test	1048:1070	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	58	theme	swimming	1080:1087	arg1	test					1089:1092	forced swimming test	1073:1092	forced swimming test	1073:1092	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	7	59	theme	gene	972:975	arg1	analysis					952:959	sequencing analysis	941:959	sequencing analysis of the 16S gene	941:975	Behavioral indicators and serum parameters were measured, and gut microbiota were identified by sequencing analysis of the 16S gene.					
33987106	4	60	theme	depression-like	517:531	arg1	behaviors					533:541	depression-like behaviors	517:541	depression-like behaviors induced by lipopolysaccharide (LPS)	517:577	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	5	61	theme	standard	642:649	arg1	chow					662:665	standard laboratory chow	642:665	standard laboratory chow	642:665	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	10	62	theme	pivotal	1381:1387	arg1	microorganisms					1389:1402	certain pivotal microorganisms	1373:1402	certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira	1373:1475	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	5	63	dep	mice	594:597	arg1	old					611:613	old	611:613	old	611:613	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	5	64	theme	laboratory	651:660	arg1	chow					662:665	standard laboratory chow	642:665	standard laboratory chow	642:665	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33987106	0	65	from	Lipopolysaccharide	112:129	arg1	Mice					134:137	Mice	134:137	Mice	134:137	Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice.					
33987106	8	66	theme	forced	1073:1078	arg1	test					1067:1070	sucrose preference test	1048:1070	sucrose preference test	1048:1070	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	66	theme	forced	1073:1078	arg1	test					1089:1092	forced swimming test	1073:1092	forced swimming test	1073:1092	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	10	67	from	variations	1337:1346	arg1	abundance					1360:1368	relative abundance	1351:1368	relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira	1351:1475	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	7	68	theme	serum	871:875	arg1	parameters					877:886	serum parameters	871:886	serum parameters	871:886	Behavioral indicators and serum parameters were measured, and gut microbiota were identified by sequencing analysis of the 16S gene.					
33987106	4	69	theme	gut-brain	429:437	arg1	axis					439:442	the gut-brain axis	425:442	the gut-brain axis	425:442	Based on the emerging perspective of the gut-brain axis, we investigated the effects of dietary CAP on gut microbes in mice with depression-like behaviors induced by lipopolysaccharide (LPS).					
33987106	7	70	theme	sequencing	941:950	arg1	analysis					952:959	sequencing analysis	941:959	sequencing analysis of the 16S gene	941:975	Behavioral indicators and serum parameters were measured, and gut microbiota were identified by sequencing analysis of the 16S gene.					
33987106	7	71	theme	Behavioral	845:854	arg1	indicators					856:865	Behavioral indicators	845:865	Behavioral indicators	845:865	Behavioral indicators and serum parameters were measured, and gut microbiota were identified by sequencing analysis of the 16S gene.					
33987106	2	72	theme	pharmacological	243:257	arg1	activities					259:268	various pharmacological activities	235:268	various pharmacological activities	235:268	It exerts various pharmacological activities, and also has potential effects on mental illness.					
33987106	0	73	theme	Capsaicin	58:66	arg1	Effects					39:45	the Preventive Effects	24:45	the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice	24:137	Gut Microbiota Mediates the Preventive Effects of Dietary Capsaicin Against Depression-Like Behavior Induced by Lipopolysaccharide in Mice.					
33987106	11	74	theme	depressive	1570:1579	arg1	behavior					1581:1588	depressive behavior	1570:1588	depressive behavior	1570:1588	Correlation analysis revealed changes in microbiota composition that was closely related to depressive behavior, 5-HT and TNF-α levels.					
33987106	2	75	theme	various	235:241	arg1	activities					259:268	various pharmacological activities	235:268	various pharmacological activities	235:268	It exerts various pharmacological activities, and also has potential effects on mental illness.					
33987106	10	76	theme	genus	1307:1311	arg1	level					1313:1317	the genus level	1303:1317	the genus level	1303:1317	Especially at the genus level, CAP enhanced the variations in relative abundance of certain pivotal microorganisms like Ruminococcus, Prevotella, Allobaculum, Sutterella, and Oscillospira.					
33987106	8	77	dep	behavior	1038:1045	arg1	test					1067:1070	sucrose preference test	1048:1070	sucrose preference test	1048:1070	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	77	dep	behavior	1038:1045	arg1	test					1089:1092	forced swimming test	1073:1092	forced swimming test	1073:1092	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	8	77	dep	behavior	1038:1045	arg1	test					1111:1114	tail suspension test	1095:1114	tail suspension test	1095:1114	This study showed that dietary CAP improved depressive-like behavior (sucrose preference test, forced swimming test, tail suspension test) and levels of 5-HT and TNF-α in serum of LPS-induced mice with depression-like behaviors.					
33987106	5	78	theme	%	696:696	arg1	CAP					698:700	0.005% CAP	691:700	0.005% CAP	691:700	C57BL/6J male mice (four weeks old) were given specific feed (standard laboratory chow or laboratory chow plus 0.005% CAP) for 4 months.					
33905556	5	0	dep	ssp	773:775	arg1	B.					763:764	B.	763:764	B.	763:764	and B. longum ssp.					
33905556	5	1	dep	B.	763:764	arg1	longum					766:771	longum	766:771	longum	766:771	and B. longum ssp.					
33905556	4	2	theme	OOM	563:565	arg1	Rochester					574:582	Rochester	574:582	Rochester	574:582	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	10	3	theme	OOM	1245:1247	arg1	OOM					1245:1247	OOM	1245:1247	OOM	1245:1247	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	10	3	theme	OOM	1245:1247	arg1	%					1240:1240	70%	1238:1240	70% of OOM	1238:1247	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	2	4	theme	early	281:285	arg1	manifestations					287:300	early manifestations	281:300	early manifestations of atopic disease	281:318	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	2	5	from	protection	213:222	arg1	age					249:251	school age	242:251	school age	242:251	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	6	6	theme	milk	822:825	arg1	HMOs					845:848	HMOs	845:848	HMOs	845:848	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	6	6	theme	milk	822:825	arg1	oligosaccharides					827:842	human milk oligosaccharides	816:842	human milk oligosaccharides (HMOs)	816:849	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	10	7	theme	Rochester	1270:1278	arg1	p < .001					1289:1296	p < .001	1289:1296	p < .001	1289:1296	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	10	7	theme	Rochester	1270:1278	arg1	infants					1280:1286	Rochester infants	1270:1286	Rochester infants (p < .001)	1270:1297	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	4	8	theme	human	629:633	arg1	samples					640:646	61 human milk samples	626:646	61 human milk samples	626:646	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	13	9	theme	children	1562:1569	arg1	%					1547:1547	35%	1545:1547	35% of Rochester children (p < .001)	1545:1580	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	9	theme	children	1562:1569	arg1	%					1532:1532	6.5%	1529:1532	6.5% of OOM	1529:1539	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	9	theme	children	1562:1569	arg1	p < .001					1572:1579	p < .001	1572:1579	p < .001	1572:1579	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	9	theme	children	1562:1569	arg1	children					1562:1569	Rochester children	1552:1569	Rochester children (p < .001)	1552:1580	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	9	theme	children	1562:1569	arg1	OOM					1537:1539	OOM	1537:1539	OOM	1537:1539	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	2	10	theme	disease	312:318	arg1	manifestations					287:300	early manifestations	281:300	early manifestations of atopic disease	281:318	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	4	11	theme	infant	609:614	arg1	stool					616:620	101 infant stool	605:620	101 infant stool	605:620	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	10	12	dep	abundant	1191:1198	arg1	detected					1226:1233	detected	1226:1233	detected in 70% of OOM compared with 21% of Rochester infants (p < .001)	1226:1297	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	10	12	dep	abundant	1191:1198	arg1	p < .001					1201:1208	p < .001	1201:1208	p < .001	1201:1208	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	14	13	theme	developing	1659:1668	arg1	countries					1670:1678	developing countries	1659:1678	developing countries	1659:1678	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	1	14	theme	farming	117:123	arg1	lifestyle					125:133	traditional farming lifestyle	105:133	traditional farming lifestyle	105:133	infantis in Old Order Mennonites with traditional farming lifestyle.					
33905556	12	15	theme	human	1425:1429	arg1	milk					1431:1434	paired human milk	1418:1434	paired human milk	1418:1434	In contrast, paired human milk was enriched with a distinct set of FAs including butyrate.					
33905556	4	16	theme	gene	674:677	arg1	sequencing					679:688	rRNA gene sequencing	669:688	rRNA gene sequencing for microbiome composition and qPCR	669:724	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	11	17	theme	higher	1337:1342	arg1	levels					1344:1349	higher levels	1337:1349	higher levels of lactate	1337:1360	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	9	18	theme	Rochester	1151:1159	arg1	infants					1161:1167	Rochester infants	1151:1167	Rochester infants	1151:1167	RESULTS At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae, and Aerococcaceae in the OOM compared with Rochester infants.					
33905556	4	19	theme	samples	640:646	arg1	cohort					550:555	a cohort	548:555	a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples	548:646	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	3	20	dep	microbiome	369:378	arg1	the					361:363	the	361:363	the	361:363	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	14	21	theme	infantis	1612:1619	arg1	colonization					1621:1632	B. infantis colonization	1609:1632	B. infantis colonization	1609:1632	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	11	22	theme	several	1366:1372	arg1	medium-					1374:1380	several medium-	1366:1380	several medium- to long/odd-chain FAs	1366:1402	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	14	23	located	found	1684:1688	arg1	risk					1708:1711	low risk	1704:1711	low risk for atopic diseases	1704:1731	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	14	23	located	found	1684:1688	arg2	rate					1601:1604	A high rate	1594:1604	A high rate	1594:1604	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	14	23	located	found	1684:1688	arg1	OOM					1697:1699	the OOM	1693:1699	the OOM	1693:1699	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	3	24	from	microbiome	369:378	arg1	infants					495:501	Rochester, NY urban/suburban infants	466:501	infants	495:501	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	24	from	microbiome	369:378	arg1	risk					511:514	high risk	506:514	high risk for atopic diseases	506:534	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	24	from	microbiome	369:378	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	24	from	microbiome	369:378	arg1	risk					457:460	low risk	453:460	low risk	453:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	25	theme	OOM	437:439	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	8	26	theme	atopic	949:954	arg1	diseases					956:963	atopic diseases	949:963	atopic diseases	949:963	Diagnoses and symptoms of atopic diseases by 3 years of age were assessed by telephone.					
33905556	3	27	theme	metabolome	384:393	arg1	composition					395:405	metabolome composition	384:405	metabolome composition	384:405	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	28	theme	Mennonite	426:434	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	0	29	theme	Infant	0:5	arg1	microbiome					11:20	Infant gut microbiome	0:20	Infant gut microbiome	0:20	Infant gut microbiome is enriched with Bifidobacterium longum ssp.					
33905556	14	30	theme	B.	1609:1610	arg1	colonization					1621:1632	B. infantis colonization	1609:1632	B. infantis colonization	1609:1632	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	7	31	theme	Fatty	852:856	arg1	FAs					865:867	FAs	865:867	FAs	865:867	Fatty acids (FAs) were analyzed in 34 stool and human 24 milk samples.					
33905556	7	31	theme	Fatty	852:856	arg1	acids					858:862	Fatty acids	852:862	Fatty acids (FAs)	852:868	Fatty acids (FAs) were analyzed in 34 stool and human 24 milk samples.					
33905556	6	32	dep	infantis	778:785	arg1	infantis					791:798	B. infantis	788:798	B. infantis	788:798	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	3	33	theme	low	453:455	arg1	risk					457:460	low risk	453:460	low risk	453:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	12	34	theme	FAs	1472:1474	arg1	FAs					1472:1474	FAs	1472:1474	FAs	1472:1474	In contrast, paired human milk was enriched with a distinct set of FAs including butyrate.					
33905556	12	34	theme	FAs	1472:1474	arg1	set					1465:1467	a distinct set	1454:1467	a distinct set of FAs including butyrate	1454:1493	In contrast, paired human milk was enriched with a distinct set of FAs including butyrate.					
33905556	3	35	theme	Old	416:418	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	2	36	theme	traditional	161:171	arg1	farms					188:192	traditional, single-family farms	161:192	traditional, single-family farms	161:192	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	14	37	theme	atopic	1717:1722	arg1	diseases					1724:1731	atopic diseases	1717:1731	atopic diseases	1717:1731	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	3	38	from	risk	457:460	arg1	infants					495:501	Rochester, NY urban/suburban infants	466:501	infants	495:501	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	38	from	risk	457:460	arg1	microbiome					369:378	gut microbiome	365:378	gut microbiome	365:378	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	38	from	risk	457:460	arg1	composition					395:405	metabolome composition	384:405	metabolome composition	384:405	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	38	from	risk	457:460	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	9	39	theme	2 months	1038:1045	arg1	age					1031:1033	a median age	1022:1033	a median age of 2 months	1022:1045	RESULTS At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae, and Aerococcaceae in the OOM compared with Rochester infants.					
33905556	9	39	theme	2 months	1038:1045	arg1	stool					1048:1052	stool	1048:1052	stool	1048:1052	RESULTS At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae, and Aerococcaceae in the OOM compared with Rochester infants.					
33905556	4	40	theme	Rochester	574:582	arg1	pairs					598:602	65 OOM and 39 Rochester mother-infant pairs	560:602	65 OOM and 39 Rochester mother-infant pairs	560:602	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	3	41	theme	urban/suburban	480:493	arg1	infants					495:501	Rochester, NY urban/suburban infants	466:501	infants	495:501	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	2	42	dep	traditional	161:171	arg1	single-family					174:186	single-family	174:186	single-family	174:186	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	11	43	theme	long/odd-chain	1385:1398	arg1	FAs					1400:1402	long/odd-chain FAs	1385:1402	long/odd-chain FAs	1385:1402	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	3	44	theme	atopic	520:525	arg1	diseases					527:534	atopic diseases	520:534	atopic diseases	520:534	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	6	45	theme	oligosaccharides	827:842	arg1	infantis					778:785	infantis	778:785	infantis (B. infantis)	778:799	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	6	45	theme	oligosaccharides	827:842	arg1	consumer					804:811	a consumer	802:811	a consumer of human milk oligosaccharides (HMOs)	802:849	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	10	46	dep	detected	1226:1233	arg1	compared					1249:1256	compared	1249:1256	compared with 21% of Rochester infants (p < .001)	1249:1297	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	6	47	theme	human	816:820	arg1	HMOs					845:848	HMOs	845:848	HMOs	845:848	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	6	47	theme	human	816:820	arg1	oligosaccharides					827:842	human milk oligosaccharides	816:842	human milk oligosaccharides (HMOs)	816:849	infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs).					
33905556	4	48	theme	stool	616:620	arg1	cohort					550:555	a cohort	548:555	a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples	548:646	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	13	49	theme	Rochester	1552:1560	arg1	children					1562:1569	Rochester children	1552:1569	Rochester children (p < .001)	1552:1580	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	49	theme	Rochester	1552:1560	arg1	p < .001					1572:1579	p < .001	1572:1579	p < .001	1572:1579	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	2	50	theme	atopic	305:310	arg1	disease					312:318	atopic disease	305:318	atopic disease	305:318	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	9	51	from	age	1031:1033	arg1	RESULTS					1011:1017	RESULTS	1011:1017	RESULTS At a median age of 2 months, stool	1011:1052	RESULTS At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae, and Aerococcaceae in the OOM compared with Rochester infants.					
33905556	14	52	dep	CONCLUSION	1583:1592	arg1	found					1684:1688	found	1684:1688	is found in the OOM at low risk for atopic diseases	1681:1731	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	2	53	theme	school	242:247	arg1	age					249:251	school age	242:251	school age	242:251	BACKGROUND Growing up on traditional, single-family farms is associated with protection against asthma in school age, but the mechanisms against early manifestations of atopic disease are largely unknown.					
33905556	12	54	theme	paired	1418:1423	arg1	milk					1431:1434	paired human milk	1418:1434	paired human milk	1418:1434	In contrast, paired human milk was enriched with a distinct set of FAs including butyrate.					
33905556	1	55	theme	traditional	105:115	arg1	lifestyle					125:133	traditional farming lifestyle	105:133	traditional farming lifestyle	105:133	infantis in Old Order Mennonites with traditional farming lifestyle.					
33905556	7	56	theme	milk	909:912	arg1	samples					914:920	34 stool and human 24 milk samples	887:920	34 stool and human 24 milk samples	887:920	Fatty acids (FAs) were analyzed in 34 stool and human 24 milk samples.					
33905556	4	57	theme	rRNA	669:672	arg1	sequencing					679:688	rRNA gene sequencing	669:688	rRNA gene sequencing for microbiome composition and qPCR	669:724	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	7	58	theme	human	900:904	arg1	samples					914:920	34 stool and human 24 milk samples	887:920	34 stool and human 24 milk samples	887:920	Fatty acids (FAs) were analyzed in 34 stool and human 24 milk samples.					
33905556	14	59	theme	similar	1635:1641	arg1	rate					1601:1604	A high rate	1594:1604	A high rate	1594:1604	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	4	60	theme	milk	635:638	arg1	samples					640:646	61 human milk samples	626:646	61 human milk samples	626:646	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	11	61	theme	lactate	1354:1360	arg1	medium-					1374:1380	several medium-	1366:1380	several medium- to long/odd-chain FAs	1366:1402	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	11	61	theme	lactate	1354:1360	arg1	levels					1344:1349	higher levels	1337:1349	higher levels of lactate	1337:1360	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	7	62	theme	stool	890:894	arg1	samples					914:920	34 stool and human 24 milk samples	887:920	34 stool and human 24 milk samples	887:920	Fatty acids (FAs) were analyzed in 34 stool and human 24 milk samples.					
33905556	14	63	theme	colonization	1621:1632	arg1	rate					1601:1604	A high rate	1594:1604	A high rate	1594:1604	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	3	64	from	risk	511:514	arg1	infants					495:501	Rochester, NY urban/suburban infants	466:501	infants	495:501	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	64	from	risk	511:514	arg1	microbiome					369:378	gut microbiome	365:378	gut microbiome	365:378	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	64	from	risk	511:514	arg1	composition					395:405	metabolome composition	384:405	metabolome composition	384:405	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	64	from	risk	511:514	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	10	65	theme	infants	1280:1286	arg1	p < .001					1289:1296	p < .001	1289:1296	p < .001	1289:1296	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	10	65	theme	infants	1280:1286	arg1	%					1265:1265	21%	1263:1265	21% of Rochester infants (p < .001)	1263:1297	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	10	65	theme	infants	1280:1286	arg1	infants					1280:1286	Rochester infants	1270:1286	Rochester infants (p < .001)	1270:1297	B. infantis was more abundant (p < .001) and prevalent, detected in 70% of OOM compared with 21% of Rochester infants (p < .001).					
33905556	3	66	theme	gut	365:367	arg1	microbiome					369:378	gut microbiome	365:378	gut microbiome	365:378	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	13	67	theme	OOM	1537:1539	arg1	%					1547:1547	35%	1545:1547	35% of Rochester children (p < .001)	1545:1580	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	67	theme	OOM	1537:1539	arg1	%					1532:1532	6.5%	1529:1532	6.5% of OOM	1529:1539	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	67	theme	OOM	1537:1539	arg1	p < .001					1572:1579	p < .001	1572:1579	p < .001	1572:1579	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	67	theme	OOM	1537:1539	arg1	children					1562:1569	Rochester children	1552:1569	Rochester children (p < .001)	1552:1580	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	13	67	theme	OOM	1537:1539	arg1	OOM					1537:1539	OOM	1537:1539	OOM	1537:1539	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	8	68	theme	diseases	956:963	arg1	symptoms					937:944	symptoms	937:944	symptoms	937:944	Diagnoses and symptoms of atopic diseases by 3 years of age were assessed by telephone.					
33905556	8	68	theme	diseases	956:963	arg1	Diagnoses					923:931	Diagnoses	923:931	Diagnoses	923:931	Diagnoses and symptoms of atopic diseases by 3 years of age were assessed by telephone.					
33905556	4	69	theme	pairs	598:602	arg1	cohort					550:555	a cohort	548:555	a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples	548:646	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	0	70	theme	gut	7:9	arg1	microbiome					11:20	Infant gut microbiome	0:20	Infant gut microbiome	0:20	Infant gut microbiome is enriched with Bifidobacterium longum ssp.					
33905556	3	71	from	composition	395:405	arg1	infants					495:501	Rochester, NY urban/suburban infants	466:501	infants	495:501	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	71	from	composition	395:405	arg1	risk					511:514	high risk	506:514	high risk for atopic diseases	506:534	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	71	from	composition	395:405	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	3	71	from	composition	395:405	arg1	risk					457:460	low risk	453:460	low risk	453:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	1	72	theme	Old	79:81	arg1	Order					83:87	Old Order	79:87	Old Order	79:87	infantis in Old Order Mennonites with traditional farming lifestyle.					
33905556	4	73	from	METHODS	537:543	arg1	cohort					550:555	a cohort	548:555	a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples	548:646	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	8	74	theme	age	979:981	arg1	3 years					968:974	3 years	968:974	3 years of age	968:981	Diagnoses and symptoms of atopic diseases by 3 years of age were assessed by telephone.					
33905556	12	75	theme	distinct	1456:1463	arg1	FAs					1472:1474	FAs	1472:1474	FAs	1472:1474	In contrast, paired human milk was enriched with a distinct set of FAs including butyrate.					
33905556	12	75	theme	distinct	1456:1463	arg1	set					1465:1467	a distinct set	1454:1467	a distinct set of FAs including butyrate	1454:1493	In contrast, paired human milk was enriched with a distinct set of FAs including butyrate.					
33905556	4	76	theme	microbiome	694:703	arg1	composition					705:715	microbiome composition	694:715	microbiome composition	694:715	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	14	77	theme	high	1596:1599	arg1	rate					1601:1604	A high rate	1594:1604	A high rate	1594:1604	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	3	78	theme	Order	420:424	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	13	79	theme	Atopic	1496:1501	arg1	diseases					1503:1510	Atopic diseases	1496:1510	Atopic diseases	1496:1510	Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p < .001).					
33905556	0	80	theme	longum	55:60	arg1	ssp					62:64	Bifidobacterium longum ssp	39:64	Bifidobacterium longum ssp	39:64	Infant gut microbiome is enriched with Bifidobacterium longum ssp.					
33905556	3	81	theme	rural	410:414	arg1	infants					442:448	rural Old Order Mennonite (OOM) infants	410:448	rural Old Order Mennonite (OOM) infants at low risk	410:460	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	9	82	theme	median	1024:1029	arg1	age					1031:1033	a median age	1022:1033	a median age of 2 months	1022:1045	RESULTS At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae, and Aerococcaceae in the OOM compared with Rochester infants.					
33905556	9	82	theme	median	1024:1029	arg1	stool					1048:1052	stool	1048:1052	stool	1048:1052	RESULTS At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae, and Aerococcaceae in the OOM compared with Rochester infants.					
33905556	4	83	theme	39	571:572	arg1	pairs					598:602	65 OOM and 39 Rochester mother-infant pairs	560:602	65 OOM and 39 Rochester mother-infant pairs	560:602	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	4	84	theme	Bifidobacterium	738:752	arg1	spp					754:756	Bifidobacterium spp	738:756	Bifidobacterium spp	738:756	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	3	85	theme	Rochester	466:474	arg1	infants					495:501	Rochester, NY urban/suburban infants	466:501	infants	495:501	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	11	86	contain	had	1333:1335	arg1	Stool					1300:1304	Stool	1300:1304	Stool colonized with B. infantis	1300:1331	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	11	86	contain	had	1333:1335	arg2	levels					1344:1349	higher levels	1337:1349	higher levels of lactate	1337:1360	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	11	86	contain	had	1333:1335	arg2	medium-					1374:1380	several medium-	1366:1380	several medium- to long/odd-chain FAs	1366:1402	Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs.					
33905556	14	87	theme	low	1704:1706	arg1	risk					1708:1711	low risk	1704:1711	low risk for atopic diseases	1704:1731	CONCLUSION A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.					
33905556	4	88	theme	mother-infant	584:596	arg1	pairs					598:602	65 OOM and 39 Rochester mother-infant pairs	560:602	65 OOM and 39 Rochester mother-infant pairs	560:602	METHODS In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp.					
33905556	3	89	theme	high	506:509	arg1	risk					511:514	high risk	506:514	high risk for atopic diseases	506:534	We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases.					
33905556	1	90	from	infantis	67:74	arg1	Order					83:87	Old Order	79:87	Old Order	79:87	infantis in Old Order Mennonites with traditional farming lifestyle.					
34902445	4	0	theme	adsorption	946:955	arg1	values					965:970	adsorption (ΔGads) values	946:970	adsorption (ΔGads) values	946:970	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	3	1	with	accurate	851:858	arg1	coefficient					867:877	a coefficient	865:877	a coefficient of determination (R2 = 99.27%)	865:908	The model was accurate with a coefficient of determination (R2 = 99.27%).					
34902445	2	2	theme	central	811:817	arg1	design					829:834	the face-centered central composite design	793:834	the face-centered central composite design	793:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	1	3	theme	@	304:304	arg1	PANI-CS-Fe3O4					305:317	Ag@PANI-CS-Fe3O4	302:317	Ag@PANI-CS-Fe3O4	302:317	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	3	theme	@	304:304	arg1	nanoparticles					287:299	silver nanoparticles	280:299	silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al)	280:368	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	2	4	theme	face-centered	797:809	arg1	design					829:834	the face-centered central composite design	793:834	the face-centered central composite design	793:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	5	5	theme	nanocomposite	1155:1167	arg1	concentration					1169:1181	5 mg/L nanocomposite concentration	1148:1181	5 mg/L nanocomposite concentration	1148:1181	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	4	6	theme	HCl	1053:1055	arg1	solution					1057:1064	5 M HCl solution	1049:1064	5 M HCl solution	1049:1064	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	1	7	theme	5 M	375:377	arg1	environment					383:393	a 5 M HCl environment	373:393	a 5 M HCl environment	373:393	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	5	8	theme	intermediate	1201:1212	arg1	temperature					1214:1224	an intermediate temperature	1198:1224	an intermediate temperature of 304.8 K.	1198:1236	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	1	9	theme	Current	451:457	arg1	Spectroscopy					474:485	Current (AC)-Impedance Spectroscopy	451:485	Current (AC)-Impedance Spectroscopy (IS)	451:490	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	4	10	theme	isotherm	1023:1030	arg1	process					1032:1038	Freundlich adsorption isotherm process	1001:1038	Freundlich adsorption isotherm process	1001:1038	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	1	11	theme	HCl	379:381	arg1	environment					383:393	a 5 M HCl environment	373:393	a 5 M HCl environment	373:393	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	5	12	theme	higher	1355:1360	arg1	temperature					1362:1372	higher temperature	1355:1372	higher temperature	1355:1372	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	3	13	theme	determination	882:894	arg1	coefficient					867:877	a coefficient	865:877	a coefficient of determination (R2 = 99.27%)	865:908	The model was accurate with a coefficient of determination (R2 = 99.27%).					
34902445	1	14	theme	AC	460:461	arg1	Spectroscopy					474:485	Current (AC)-Impedance Spectroscopy	451:485	Current (AC)-Impedance Spectroscopy (IS)	451:490	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	6	15	theme	experimental	1464:1475	arg1	observations					1477:1488	the experimental observations	1460:1488	the experimental observations	1460:1488	The results obtained by the RSM model are in agreement with the experimental observations.					
34902445	4	16	theme	ΔGads	958:962	arg1	values					965:970	adsorption (ΔGads) values	946:970	adsorption (ΔGads) values	946:970	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	2	17	theme	Surface	669:675	arg1	RSM					690:692	RSM	690:692	RSM	690:692	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	2	17	theme	Surface	669:675	arg1	Methodology					677:687	the Response Surface Methodology	656:687	the Response Surface Methodology (RSM)	656:693	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	1	18	theme	-Impedance	463:472	arg1	Spectroscopy					474:485	Current (AC)-Impedance Spectroscopy	451:485	Current (AC)-Impedance Spectroscopy (IS)	451:490	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	19	theme	Scanning	545:552	arg1	Microscopy					563:572	Scanning Electron Microscopy	545:572	Scanning Electron Microscopy (SEM) techniques	545:589	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	19	theme	Scanning	545:552	arg1	SEM					575:577	SEM	575:577	SEM	575:577	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	20	theme	synthesized	209:219	arg1	nanocomposite					251:263	newly synthesized magnetic polyaniline-Chitosan nanocomposite	203:263	newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al)	203:368	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	5	21	theme	5 mg/L	1148:1153	arg1	concentration					1169:1181	5 mg/L nanocomposite concentration	1148:1181	5 mg/L nanocomposite concentration	1148:1181	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	2	22	theme	protection	596:605	arg1	efficiency					607:616	The protection efficiency	592:616	The protection efficiency (PE)	592:621	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	2	22	theme	protection	596:605	arg1	PE					619:620	PE	619:620	PE	619:620	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	4	23	theme	values	965:970	arg1	energy					936:941	The negative Gibb's free energy	911:941	The negative Gibb's free energy of adsorption (ΔGads) values	911:970	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	1	24	theme	Electron	554:561	arg1	Microscopy					563:572	Scanning Electron Microscopy	545:572	Scanning Electron Microscopy (SEM) techniques	545:589	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	24	theme	Electron	554:561	arg1	SEM					575:577	SEM	575:577	SEM	575:577	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	25	theme	magnetic	221:228	arg1	nanocomposite					251:263	newly synthesized magnetic polyaniline-Chitosan nanocomposite	203:263	newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al)	203:368	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	0	26	theme	performance	31:41	arg1	Optimization					0:11	Optimization	0:11	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver	0:116	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	1	27	theme	robust	325:330	arg1	inhibitor					342:350	a robust corrosion inhibitor	323:350	a robust corrosion inhibitor	323:350	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	0	28	theme	acidizing	151:159	arg1	environment					161:171	simulated acidizing environment	141:171	simulated acidizing environment using RSM	141:181	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	1	29	theme	Microscopy	563:572	arg1	techniques					580:589	Scanning Electron Microscopy (SEM) techniques	545:589	Scanning Electron Microscopy (SEM) techniques	545:589	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	30	theme	polyaniline-Chitosan	230:249	arg1	nanocomposite					251:263	newly synthesized magnetic polyaniline-Chitosan nanocomposite	203:263	newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al)	203:368	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	0	31	theme	anti-corrosion	16:29	arg1	performance					31:41	anti-corrosion performance	16:41	anti-corrosion performance	16:41	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	1	32	theme	corrosion	332:340	arg1	inhibitor					342:350	a robust corrosion inhibitor	323:350	a robust corrosion inhibitor	323:350	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	0	33	theme	simulated	141:149	arg1	environment					161:171	simulated acidizing environment	141:171	simulated acidizing environment using RSM	141:181	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	5	34	theme	PE	1270:1271	arg1	sensitivity					1255:1265	the sensitivity	1251:1265	the sensitivity of PE	1251:1271	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	1	35	theme	nanocomposite	251:263	arg1	suitability					188:198	The suitability	184:198	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment	184:393	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	0	36	theme	novel	46:50	arg1	nanocomposite					82:94	novel magnetic polyaniline-Chitosan nanocomposite	46:94	novel magnetic polyaniline-Chitosan nanocomposite decorated with silver	46:116	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	5	37	theme	low	1304:1306	arg1	favorable					1329:1337	favorable	1329:1337	favorable	1329:1337	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	37	theme	low	1304:1306	arg1	303 K					1320:1324	the low temperature 303 K	1300:1324	the low temperature 303 K	1300:1324	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	4	38	theme	process	1032:1038	arg1	spontaneity					986:996	the spontaneity	982:996	the spontaneity of Freundlich adsorption isotherm process on Al	982:1044	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	2	39	theme	temperature	736:746	arg1	terms					727:731	terms	727:731	terms of temperature, nanocomposite concentration, and time using the face-centered central composite design	727:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	5	40	theme	temperature	1308:1318	arg1	favorable					1329:1337	favorable	1329:1337	favorable	1329:1337	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	40	theme	temperature	1308:1318	arg1	303 K					1320:1324	the low temperature 303 K	1300:1324	the low temperature 303 K	1300:1324	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	41	theme	protection	1111:1120	arg1	%					1142:1142	of 97.88%	1134:1142	of 97.88%	1134:1142	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	41	theme	protection	1111:1120	arg1	efficiency					1122:1131	maximum protection efficiency	1103:1131	maximum protection efficiency (of 97.88%)	1103:1143	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	1	42	theme	Weight	421:426	arg1	WL					434:435	WL	434:435	WL	434:435	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	42	theme	Weight	421:426	arg1	Loss					428:431	Weight Loss	421:431	Weight Loss (WL)	421:436	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	2	43	from	relation	715:722	arg1	terms					727:731	terms	727:731	terms of temperature, nanocomposite concentration, and time using the face-centered central composite design	727:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	2	44	theme	concentration	763:775	arg1	terms					727:731	terms	727:731	terms of temperature, nanocomposite concentration, and time using the face-centered central composite design	727:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	0	45	theme	polyaniline-Chitosan	61:80	arg1	nanocomposite					82:94	novel magnetic polyaniline-Chitosan nanocomposite	46:94	novel magnetic polyaniline-Chitosan nanocomposite decorated with silver	46:116	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	4	46	theme	negative	915:922	arg1	energy					936:941	The negative Gibb's free energy	911:941	The negative Gibb's free energy of adsorption (ΔGads) values	911:970	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	6	47	theme	RSM	1428:1430	arg1	model					1432:1436	the RSM model	1424:1436	the RSM model	1424:1436	The results obtained by the RSM model are in agreement with the experimental observations.					
34902445	4	48	theme	free	931:934	arg1	energy					936:941	The negative Gibb's free energy	911:941	The negative Gibb's free energy of adsorption (ΔGads) values	911:970	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	6	49	with	agreement	1445:1453	arg1	observations					1477:1488	the experimental observations	1460:1488	the experimental observations	1460:1488	The results obtained by the RSM model are in agreement with the experimental observations.					
34902445	0	50	theme	magnetic	52:59	arg1	nanocomposite					82:94	novel magnetic polyaniline-Chitosan nanocomposite	46:94	novel magnetic polyaniline-Chitosan nanocomposite decorated with silver	46:116	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	1	51	from	suitability	188:198	arg1	environment					383:393	a 5 M HCl environment	373:393	a 5 M HCl environment	373:393	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	5	52	theme	304.8 K.	1229:1236	arg1	concentration					1169:1181	5 mg/L nanocomposite concentration	1148:1181	5 mg/L nanocomposite concentration	1148:1181	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	52	theme	304.8 K.	1229:1236	arg1	temperature					1214:1224	an intermediate temperature	1198:1224	an intermediate temperature of 304.8 K.	1198:1236	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	52	theme	304.8 K.	1229:1236	arg1	time					1188:1191	1 h time	1184:1191	1 h time	1184:1191	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	4	53	from	spontaneity	986:996	arg1	Al					1043:1044	Al	1043:1044	Al	1043:1044	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	1	54	theme	Potentiontiodynamic	493:511	arg1	polarization					513:524	Potentiontiodynamic polarization	493:524	Potentiontiodynamic polarization (Tafel plots)	493:538	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	54	theme	Potentiontiodynamic	493:511	arg1	plots					533:537	Tafel plots	527:537	Tafel plots	527:537	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	2	55	theme	time	782:785	arg1	terms					727:731	terms	727:731	terms of temperature, nanocomposite concentration, and time using the face-centered central composite design	727:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	1	56	theme	silver	280:285	arg1	PANI-CS-Fe3O4					305:317	Ag@PANI-CS-Fe3O4	302:317	Ag@PANI-CS-Fe3O4	302:317	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	56	theme	silver	280:285	arg1	nanoparticles					287:299	silver nanoparticles	280:299	silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al)	280:368	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	4	57	theme	Freundlich	1001:1010	arg1	process					1032:1038	Freundlich adsorption isotherm process	1001:1038	Freundlich adsorption isotherm process	1001:1038	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	2	58	theme	empirical	705:713	arg1	relation					715:722	an empirical relation	702:722	an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design	702:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	2	59	theme	nanocomposite	749:761	arg1	concentration					763:775	nanocomposite concentration	749:775	nanocomposite concentration	749:775	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	4	60	theme	5 M	1049:1051	arg1	solution					1057:1064	5 M HCl solution	1049:1064	5 M HCl solution	1049:1064	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	2	61	theme	Response	660:667	arg1	RSM					690:692	RSM	690:692	RSM	690:692	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	2	61	theme	Response	660:667	arg1	Methodology					677:687	the Response Surface Methodology	656:687	the Response Surface Methodology (RSM)	656:693	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	4	62	theme	adsorption	1012:1021	arg1	process					1032:1038	Freundlich adsorption isotherm process	1001:1038	Freundlich adsorption isotherm process	1001:1038	The negative Gibb's free energy of adsorption (ΔGads) values confirmed the spontaneity of Freundlich adsorption isotherm process on Al in 5 M HCl solution.					
34902445	5	63	theme	maximum	1103:1109	arg1	%					1142:1142	of 97.88%	1134:1142	of 97.88%	1134:1142	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	5	63	theme	maximum	1103:1109	arg1	efficiency					1122:1131	maximum protection efficiency	1103:1131	maximum protection efficiency (of 97.88%)	1103:1143	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	2	64	theme	composite	819:827	arg1	design					829:834	the face-centered central composite design	793:834	the face-centered central composite design	793:834	The protection efficiency (PE) was mathematically modeled using the Response Surface Methodology (RSM) to fit an empirical relation in terms of temperature, nanocomposite concentration, and time using the face-centered central composite design.					
34902445	0	65	theme	nanocomposite	82:94	arg1	Optimization					0:11	Optimization	0:11	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver	0:116	Optimization of anti-corrosion performance of novel magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles on Al in simulated acidizing environment using RSM.					
34902445	5	66	theme	optimization	1071:1082	arg1	simulation					1084:1093	The optimization simulation	1067:1093	The optimization simulation	1067:1093	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	1	67	theme	Tafel	527:531	arg1	polarization					513:524	Potentiontiodynamic polarization	493:524	Potentiontiodynamic polarization (Tafel plots)	493:538	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	67	theme	Tafel	527:531	arg1	plots					533:537	Tafel plots	527:537	Tafel plots	527:537	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	5	68	theme	1 h	1184:1186	arg1	time					1188:1191	1 h time	1184:1191	1 h time	1184:1191	The optimization simulation yielded maximum protection efficiency (of 97.88%) at 5 mg/L nanocomposite concentration, 1 h time, and an intermediate temperature of 304.8 K. Furthermore, the sensitivity of PE was evaluated to find that the low temperature 303 K is favorable for PE, whereas higher temperature will act adversely on PE.					
34902445	1	69	dep	Spectroscopy	474:485	arg1	IS					488:489	IS	488:489	IS	488:489	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	70	theme	Ag	302:303	arg1	PANI-CS-Fe3O4					305:317	Ag@PANI-CS-Fe3O4	302:317	Ag@PANI-CS-Fe3O4	302:317	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
34902445	1	70	theme	Ag	302:303	arg1	nanoparticles					287:299	silver nanoparticles	280:299	silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al)	280:368	The suitability of newly synthesized magnetic polyaniline-Chitosan nanocomposite decorated with silver nanoparticles (Ag@PANI-CS-Fe3O4) as a robust corrosion inhibitor for Aluminum (Al) in a 5 M HCl environment has been investigated via Weight Loss (WL), Alternating Current (AC)-Impedance Spectroscopy (IS), Potentiontiodynamic polarization (Tafel plots), and Scanning Electron Microscopy (SEM) techniques.					
32673948	0	0	theme	polyphenols	87:97	arg1	separation					69:78	effective separation	59:78	effective separation of tea polyphenols from green tea	59:112	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	2	1	theme	resulting	429:437	arg1	membrane					439:446	the resulting membrane	425:446	the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	425:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	4	2	theme	mixture	823:829	arg1	solution					809:816	an aqueous solution	798:816	an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane	798:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	5	3	theme	Freundlich	1041:1050	arg1	model					1052:1056	Freundlich model	1041:1056	Freundlich model	1041:1056	The experimental data fit well with pseudo-second-order model and Freundlich model.					
32673948	1	4	theme	liquid	240:245	arg1	solution					188:195	gelatin solution	180:195	gelatin solution	180:195	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	4	theme	liquid	240:245	arg1	complex					223:229	an inclusion complex	210:229	an inclusion complex of ionic liquid	210:245	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	4	theme	liquid	240:245	arg1	beta-cyclodextrin					256:272	beta-cyclodextrin	256:272	beta-cyclodextrin (β-CD)	256:279	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	4	theme	liquid	240:245	arg1	porogen					198:204	porogen	198:204	porogen	198:204	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	4	theme	liquid	240:245	arg1	β-CD					275:278	β-CD	275:278	β-CD	275:278	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	4	theme	liquid	240:245	arg1	IL					248:249	IL	248:249	IL	248:249	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	0	5	theme	tea	83:85	arg1	polyphenols					87:97	tea polyphenols	83:97	tea polyphenols	83:97	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	3	6	theme	newly-developed	612:626	arg1	film					628:631	Then the newly-developed film	603:631	Then the newly-developed film	603:631	Then the newly-developed film was comprehensively characterized by scanning electron microscopy, conductivity, thermogravimetry and spectral analysis.					
32673948	4	7	from	theophylline	842:853	arg1	membrane					870:877	IL@β-CD-Gel membrane	858:877	IL@β-CD-Gel membrane	858:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	2	8	theme	target	585:590	arg1	molecules					592:600	the target molecules	581:600	the target molecules	581:600	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	2	9	theme	dicationic	469:478	arg1	2C3[l-pro					516:524	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	1	10	theme	selective	313:321	arg1	separation					323:332	selective separation	313:332	selective separation of tea polyphenols (TPs) from green tea crude extracts	313:387	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	4	11	theme	TPs	834:836	arg1	mixture					823:829	a mixture	821:829	a mixture of TPs	821:836	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	4	11	theme	TPs	834:836	arg1	theophylline					842:853	theophylline	842:853	theophylline on IL@β-CD-Gel membrane	842:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	3	12	theme	scanning	670:677	arg1	microscopy					688:697	scanning electron microscopy	670:697	scanning electron microscopy	670:697	Then the newly-developed film was comprehensively characterized by scanning electron microscopy, conductivity, thermogravimetry and spectral analysis.					
32673948	4	13	theme	%	972:972	arg1	percentages					952:962	removal percentages	944:962	removal percentages of 94.38%	944:972	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	6	14	theme	separation	1122:1131	arg1	technology					1099:1108	a new technology	1093:1108	a new technology of membrane separation for selective adsorption of TPs	1093:1163	By using this composite material, a new technology of membrane separation for selective adsorption of TPs was finally established.					
32673948	3	15	theme	electron	679:686	arg1	microscopy					688:697	scanning electron microscopy	670:697	scanning electron microscopy	670:697	Then the newly-developed film was comprehensively characterized by scanning electron microscopy, conductivity, thermogravimetry and spectral analysis.					
32673948	4	16	theme	aqueous	801:807	arg1	solution					809:816	an aqueous solution	798:816	an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane	798:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	6	17	theme	membrane	1113:1120	arg1	separation					1122:1131	membrane separation	1113:1131	membrane separation	1113:1131	By using this composite material, a new technology of membrane separation for selective adsorption of TPs was finally established.					
32673948	4	18	theme	@	860:860	arg1	membrane					870:877	IL@β-CD-Gel membrane	858:877	IL@β-CD-Gel membrane	858:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	4	19	theme	adsorption	895:904	arg1	capacity					906:913	the adsorption capacity	891:913	the adsorption capacity for TPs	891:921	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	1	20	theme	tea	337:339	arg1	TPs					354:356	TPs	354:356	TPs	354:356	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	20	theme	tea	337:339	arg1	polyphenols					341:351	tea polyphenols	337:351	tea polyphenols (TPs)	337:357	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	0	21	theme	liquid	6:11	arg1	β-cyclodextrin-gelatin					13:34	Ionic liquid@β-cyclodextrin-gelatin	0:34	Ionic liquid@β-cyclodextrin-gelatin	0:34	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	1	22	theme	polyphenols	341:351	arg1	separation					323:332	selective separation	313:332	selective separation of tea polyphenols (TPs) from green tea crude extracts	313:387	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	4	23	from	solution	809:816	arg1	membrane					870:877	IL@β-CD-Gel membrane	858:877	IL@β-CD-Gel membrane	858:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	4	23	from	solution	809:816	arg1	adsorption					782:791	the adsorption	778:791	the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane	778:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	1	24	theme	gelatin	180:186	arg1	solution					188:195	gelatin solution	180:195	gelatin solution	180:195	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	0	25	theme	Ionic	0:4	arg1	β-cyclodextrin-gelatin					13:34	Ionic liquid@β-cyclodextrin-gelatin	0:34	Ionic liquid@β-cyclodextrin-gelatin	0:34	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	2	26	theme	excellent	537:545	arg1	performance					547:557	the excellent performance	533:557	the excellent performance for the enrichment of the target molecules	533:600	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	5	27	theme	experimental	979:990	arg1	data					992:995	The experimental data	975:995	The experimental data	975:995	The experimental data fit well with pseudo-second-order model and Freundlich model.					
32673948	5	28	theme	pseudo-second-order	1011:1029	arg1	model					1031:1035	pseudo-second-order model	1011:1035	pseudo-second-order model	1011:1035	The experimental data fit well with pseudo-second-order model and Freundlich model.					
32673948	4	29	theme	β-CD-Gel	861:868	arg1	membrane					870:877	IL@β-CD-Gel membrane	858:877	IL@β-CD-Gel membrane	858:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	2	30	theme	molecules	592:600	arg1	enrichment					567:576	the enrichment	563:576	the enrichment of the target molecules	563:600	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	0	31	theme	@	12:12	arg1	β-cyclodextrin-gelatin					13:34	Ionic liquid@β-cyclodextrin-gelatin	0:34	Ionic liquid@β-cyclodextrin-gelatin	0:34	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	2	32	theme	salt	505:508	arg1	2C3[l-pro					516:524	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	6	33	theme	new	1095:1097	arg1	technology					1099:1108	a new technology	1093:1108	a new technology of membrane separation for selective adsorption of TPs	1093:1163	By using this composite material, a new technology of membrane separation for selective adsorption of TPs was finally established.					
32673948	4	34	theme	removal	944:950	arg1	percentages					952:962	removal percentages	944:962	removal percentages of 94.38%	944:972	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	6	35	theme	composite	1073:1081	arg1	material					1083:1090	this composite material	1068:1090	this composite material	1068:1090	By using this composite material, a new technology of membrane separation for selective adsorption of TPs was finally established.					
32673948	2	36	theme	proline	497:503	arg1	2C3[l-pro					516:524	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	4	37	from	membrane	870:877	arg1	solution					809:816	an aqueous solution	798:816	an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane	798:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	3	38	theme	spectral	735:742	arg1	analysis					744:751	spectral analysis	735:751	spectral analysis	735:751	Then the newly-developed film was comprehensively characterized by scanning electron microscopy, conductivity, thermogravimetry and spectral analysis.					
32673948	2	39	theme	N-vinylimidazole	480:495	arg1	2C3[l-pro					516:524	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	0	40	theme	effective	59:67	arg1	separation					69:78	effective separation	59:78	effective separation of tea polyphenols from green tea	59:112	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	4	41	theme	theophylline	842:853	arg1	solution					809:816	an aqueous solution	798:816	an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane	798:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	4	42	from	mixture	823:829	arg1	membrane					870:877	IL@β-CD-Gel membrane	858:877	IL@β-CD-Gel membrane	858:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	2	43	theme	2C3[l-pro	516:524	arg1	IL					463:464	the IL	459:464	the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	459:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	1	44	theme	green	364:368	arg1	extracts					380:387	green tea crude extracts	364:387	green tea crude extracts	364:387	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	0	45	from	tea	110:112	arg1	separation					69:78	effective separation	59:78	effective separation of tea polyphenols from green tea	59:112	Ionic liquid@β-cyclodextrin-gelatin composite membrane for effective separation of tea polyphenols from green tea.					
32673948	1	46	theme	new	117:119	arg1	kind					121:124	A new kind	115:124	A new kind of multi-component membrane	115:152	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	47	theme	simple	286:291	arg1	manner					302:307	a simple physical manner	284:307	a simple physical manner	284:307	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	4	48	theme	IL	858:859	arg1	membrane					870:877	IL@β-CD-Gel membrane	858:877	IL@β-CD-Gel membrane	858:877	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	1	49	theme	tea	370:372	arg1	extracts					380:387	green tea crude extracts	364:387	green tea crude extracts	364:387	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	6	50	theme	TPs	1161:1163	arg1	adsorption					1147:1156	selective adsorption	1137:1156	selective adsorption of TPs	1137:1163	By using this composite material, a new technology of membrane separation for selective adsorption of TPs was finally established.					
32673948	2	51	contain	containing	448:457	arg1	membrane					439:446	the resulting membrane	425:446	the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	425:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	2	51	contain	containing	448:457	arg2	IL					463:464	the IL	459:464	the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	459:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	1	52	theme	inclusion	213:221	arg1	complex					223:229	an inclusion complex	210:229	an inclusion complex of ionic liquid	210:245	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	2	53	theme	[VIm	511:514	arg1	2C3[l-pro					516:524	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	469:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	1	54	theme	crude	374:378	arg1	extracts					380:387	green tea crude extracts	364:387	green tea crude extracts	364:387	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	2	55	contain	had	529:531	arg1	membrane					439:446	the resulting membrane	425:446	the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2)	425:527	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	2	55	contain	had	529:531	arg2	performance					547:557	the excellent performance	533:557	the excellent performance for the enrichment of the target molecules	533:600	After screening, it was found that the resulting membrane containing the IL of dicationic N-vinylimidazole proline salt ([VIm]2C3[l-pro]2) had the excellent performance for the enrichment of the target molecules.					
32673948	4	56	theme	driving	769:775	arg1	pressure					760:767	pressure driving	760:775	pressure driving	760:775	Under pressure driving, the adsorption from an aqueous solution of a mixture of TPs and theophylline on IL@β-CD-Gel membrane showed that the adsorption capacity for TPs was 303.45 mg/g with removal percentages of 94.38%.					
32673948	1	57	theme	multi-component	129:143	arg1	membrane					145:152	multi-component membrane	129:152	multi-component membrane	129:152	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	58	from	extracts	380:387	arg1	separation					323:332	selective separation	313:332	selective separation of tea polyphenols (TPs) from green tea crude extracts	313:387	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	1	59	theme	physical	293:300	arg1	manner					302:307	a simple physical manner	284:307	a simple physical manner	284:307	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
32673948	6	60	theme	selective	1137:1145	arg1	adsorption					1147:1156	selective adsorption	1137:1156	selective adsorption of TPs	1137:1163	By using this composite material, a new technology of membrane separation for selective adsorption of TPs was finally established.					
32673948	1	61	theme	membrane	145:152	arg1	kind					121:124	A new kind	115:124	A new kind of multi-component membrane	115:152	A new kind of multi-component membrane was prepared by combining gelatin solution, porogen and an inclusion complex of ionic liquid (IL) and beta-cyclodextrin (β-CD) in a simple physical manner for selective separation of tea polyphenols (TPs) from green tea crude extracts.					
33189957	2	0	theme	Sil-VMBD	617:624	arg1	material					626:633	the synthesized Sil-VMBD material	601:633	the synthesized Sil-VMBD material	601:633	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	4	1	theme	phase	846:850	arg1	mechanism					807:815	the retention mechanism	793:815	the retention mechanism of the multi-mode stationary phase	793:850	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	2	2	theme	materials	458:466	arg1	identifications					404:418	identifications	404:418	identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis	404:598	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	1	3	theme	6-	329:330	arg1	tosylate					365:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	3	theme	6-	329:330	arg1	monomers					388:395	functional monomers	377:395	functional monomers	377:395	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	0	4	theme	achiral	84:90	arg1	functions					114:122	achiral and chiral separation functions	84:122	achiral and chiral separation functions	84:122	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	6	5	theme	ibuprofen	1072:1080	arg1	enantioseparation					1051:1067	Fast enantioseparation	1046:1067	Fast enantioseparation of ibuprofen and ketoprofen	1046:1095	Fast enantioseparation of ibuprofen and ketoprofen were obtained within 4 min under polar organic mode.					
33189957	7	6	with	phase	1360:1364	arg1	abilities					1404:1412	multi-mode and chiral separation abilities	1371:1412	multi-mode and chiral separation abilities	1371:1412	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	2	7	theme	phase	452:456	arg1	materials					458:466	stationary phase materials	441:466	stationary phase materials	441:466	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	2	8	theme	intermediates	423:435	arg1	identifications					404:418	identifications	404:418	identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis	404:598	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	0	9	theme	separation	103:112	arg1	functions					114:122	achiral and chiral separation functions	84:122	achiral and chiral separation functions	84:122	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	4	10	theme	pH	910:911	arg1	value					913:917	pH value	910:917	pH value	910:917	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	0	11	theme	chiral	96:101	arg1	functions					114:122	achiral and chiral separation functions	84:122	achiral and chiral separation functions	84:122	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	1	12	dep	interaction/ion-exchange	160:183	arg1	material					253:260	material	253:260	material	253:260	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	4	13	theme	stationary	835:844	arg1	phase					846:850	the multi-mode stationary phase	820:850	the multi-mode stationary phase	820:850	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	6	14	theme	Fast	1046:1049	arg1	enantioseparation					1051:1067	Fast enantioseparation	1046:1067	Fast enantioseparation of ibuprofen and ketoprofen	1046:1095	Fast enantioseparation of ibuprofen and ketoprofen were obtained within 4 min under polar organic mode.					
33189957	5	15	theme	styrene	983:989	arg1	oxide					991:995	styrene oxide	983:995	styrene oxide	983:995	Moreover, the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide were separated rapidly under reverse-phase mode.					
33189957	0	16	with	phase	73:77	arg1	functions					114:122	achiral and chiral separation functions	84:122	achiral and chiral separation functions	84:122	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	2	17	theme	resonance	491:499	arg1	analysis					501:508	nuclear magnetic resonance analysis	474:508	nuclear magnetic resonance analysis	474:508	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	1	18	theme	chiral	206:211	arg1	phase					247:251	chiral recognition mixed-mode stationary phase	206:251	chiral recognition mixed-mode stationary phase	206:251	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	7	19	theme	chiral	1386:1391	arg1	abilities					1404:1412	multi-mode and chiral separation abilities	1371:1412	multi-mode and chiral separation abilities	1371:1412	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	7	20	theme	various	1292:1298	arg1	analytes					1300:1307	various analytes	1292:1307	various analytes	1292:1307	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	5	21	theme	1-phenyl-1-propanol	949:967	arg1	enantiomers					934:944	the enantiomers	930:944	the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide	930:995	Moreover, the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide were separated rapidly under reverse-phase mode.					
33189957	7	22	contain	possesses	1219:1227	arg1	column					1212:1217	the as-prepared mixed-mode column	1185:1217	the as-prepared mixed-mode column	1185:1217	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	7	22	contain	possesses	1219:1227	arg2	interactions					1274:1285	reversed-phase, hydrophilic and ion-exchange interactions	1229:1285	reversed-phase, hydrophilic and ion-exchange interactions with various analytes	1229:1307	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	1	23	theme	recognition	213:223	arg1	phase					247:251	chiral recognition mixed-mode stationary phase	206:251	chiral recognition mixed-mode stationary phase	206:251	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	0	24	theme	liquid	6:11	arg1	β-cyclodextrin					28:41	Ionic liquid functionalized β-cyclodextrin	0:41	Ionic liquid functionalized β-cyclodextrin	0:41	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	2	25	theme	synthesized	605:615	arg1	material					626:633	the synthesized Sil-VMBD material	601:633	the synthesized Sil-VMBD material	601:633	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	7	26	theme	ion-exchange	1261:1272	arg1	interactions					1274:1285	reversed-phase, hydrophilic and ion-exchange interactions	1229:1285	reversed-phase, hydrophilic and ion-exchange interactions with various analytes	1229:1307	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	6	27	theme	organic	1136:1142	arg1	mode					1144:1147	polar organic mode	1130:1147	polar organic mode	1130:1147	Fast enantioseparation of ibuprofen and ketoprofen were obtained within 4 min under polar organic mode.					
33189957	3	28	theme	mixed-mode	692:701	arg1	performance					713:723	The mixed-mode retention performance	688:723	The mixed-mode retention performance	688:723	The mixed-mode retention performance was researched by hydrophobic, hydrophilic and ionic compounds.					
33189957	1	29	theme	mixed-mode	225:234	arg1	phase					247:251	chiral recognition mixed-mode stationary phase	206:251	chiral recognition mixed-mode stationary phase	206:251	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	0	30	theme	Ionic	0:4	arg1	β-cyclodextrin					28:41	Ionic liquid functionalized β-cyclodextrin	0:41	Ionic liquid functionalized β-cyclodextrin	0:41	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	1	31	theme	-cyclodextrin	351:363	arg1	tosylate					365:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	31	theme	-cyclodextrin	351:363	arg1	monomers					388:395	functional monomers	377:395	functional monomers	377:395	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	3	32	theme	retention	703:711	arg1	performance					713:723	The mixed-mode retention performance	688:723	The mixed-mode retention performance	688:723	The mixed-mode retention performance was researched by hydrophobic, hydrophilic and ionic compounds.					
33189957	1	33	theme	stationary	236:245	arg1	phase					247:251	chiral recognition mixed-mode stationary phase	206:251	chiral recognition mixed-mode stationary phase	206:251	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	2	34	theme	element	552:558	arg1	analysis					560:567	element analysis	552:567	element analysis	552:567	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	6	35	theme	polar	1130:1134	arg1	mode					1144:1147	polar organic mode	1130:1147	polar organic mode	1130:1147	Fast enantioseparation of ibuprofen and ketoprofen were obtained within 4 min under polar organic mode.					
33189957	7	36	theme	hydrophilic	1245:1255	arg1	interactions					1274:1285	reversed-phase, hydrophilic and ion-exchange interactions	1229:1285	reversed-phase, hydrophilic and ion-exchange interactions with various analytes	1229:1307	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	7	37	with	interactions	1274:1285	arg1	analytes					1300:1307	various analytes	1292:1307	various analytes	1292:1307	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	0	38	theme	functionalized	13:26	arg1	β-cyclodextrin					28:41	Ionic liquid functionalized β-cyclodextrin	0:41	Ionic liquid functionalized β-cyclodextrin	0:41	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	2	39	theme	high	673:676	arg1	pressure					678:685	high pressure	673:685	high pressure	673:685	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	4	40	theme	phase	888:892	arg1	composition					894:904	the mobile phase composition	877:904	the mobile phase composition	877:904	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	4	41	theme	multi-mode	824:833	arg1	phase					846:850	the multi-mode stationary phase	820:850	the multi-mode stationary phase	820:850	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	7	42	theme	separation	1393:1402	arg1	abilities					1404:1412	multi-mode and chiral separation abilities	1371:1412	multi-mode and chiral separation abilities	1371:1412	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	1	43	theme	functional	377:386	arg1	tosylate					365:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	43	theme	functional	377:386	arg1	monomers					388:395	functional monomers	377:395	functional monomers	377:395	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	7	44	theme	reversed-phase	1229:1242	arg1	interactions					1274:1285	reversed-phase, hydrophilic and ion-exchange interactions	1229:1285	reversed-phase, hydrophilic and ion-exchange interactions with various analytes	1229:1307	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	7	45	theme	multi-mode	1371:1380	arg1	abilities					1404:1412	multi-mode and chiral separation abilities	1371:1412	multi-mode and chiral separation abilities	1371:1412	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	2	46	dep	transform	519:527	arg1	infrared					529:536	infrared	529:536	transform infrared spectrometer	519:549	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	2	47	theme	magnetic	482:489	arg1	resonance					491:499	nuclear magnetic resonance	474:499	nuclear magnetic resonance analysis	474:508	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	5	48	theme	oxide	991:995	arg1	enantiomers					934:944	the enantiomers	930:944	the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide	930:995	Moreover, the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide were separated rapidly under reverse-phase mode.					
33189957	4	49	theme	retention	797:805	arg1	mechanism					807:815	the retention mechanism	793:815	the retention mechanism of the multi-mode stationary phase	793:850	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	3	50	theme	ionic	772:776	arg1	compounds					778:786	hydrophobic, hydrophilic and ionic compounds	743:786	hydrophobic, hydrophilic and ionic compounds	743:786	The mixed-mode retention performance was researched by hydrophobic, hydrophilic and ionic compounds.					
33189957	2	51	dep	Fourier	511:517	arg1	transform					519:527	transform	519:527	transform infrared spectrometer	519:549	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	0	52	theme	stationary	62:71	arg1	phase					73:77	stationary phase	62:77	stationary phase with achiral and chiral separation functions	62:122	Ionic liquid functionalized β-cyclodextrin and C18 mixed-mode stationary phase with achiral and chiral separation functions.					
33189957	2	53	theme	empty	654:658	arg1	column					660:665	an empty column	651:665	an empty column under high pressure	651:685	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	2	54	theme	stationary	441:450	arg1	materials					458:466	stationary phase materials	441:466	stationary phase materials	441:466	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	2	55	theme	thermogravimetric	573:589	arg1	analysis					591:598	thermogravimetric analysis	573:598	thermogravimetric analysis	573:598	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	1	56	theme	novel	127:131	arg1	RPLC/HILIC/IEC					186:199	RPLC/HILIC/IEC	186:199	RPLC/HILIC/IEC	186:199	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	56	theme	novel	127:131	arg1	interaction/ion-exchange					160:183	A novel reversed-phase/hydrophilic interaction/ion-exchange	125:183	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC)	125:200	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	7	57	theme	stationary	1349:1358	arg1	phase					1360:1364	the stationary phase	1345:1364	the stationary phase with multi-mode and chiral separation abilities	1345:1412	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	7	58	theme	as-prepared	1189:1199	arg1	column					1212:1217	the as-prepared mixed-mode column	1185:1217	the as-prepared mixed-mode column	1185:1217	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	4	59	theme	mobile	881:886	arg1	composition					894:904	the mobile phase composition	877:904	the mobile phase composition	877:904	And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value.					
33189957	3	60	theme	hydrophilic	756:766	arg1	compounds					778:786	hydrophobic, hydrophilic and ionic compounds	743:786	hydrophobic, hydrophilic and ionic compounds	743:786	The mixed-mode retention performance was researched by hydrophobic, hydrophilic and ionic compounds.					
33189957	1	61	theme	reversed-phase/hydrophilic	133:158	arg1	RPLC/HILIC/IEC					186:199	RPLC/HILIC/IEC	186:199	RPLC/HILIC/IEC	186:199	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	61	theme	reversed-phase/hydrophilic	133:158	arg1	interaction/ion-exchange					160:183	A novel reversed-phase/hydrophilic interaction/ion-exchange	125:183	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC)	125:200	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	5	62	theme	reverse-phase	1026:1038	arg1	mode					1040:1043	reverse-phase mode	1026:1043	reverse-phase mode	1026:1043	Moreover, the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide were separated rapidly under reverse-phase mode.					
33189957	7	63	theme	mixed-mode	1201:1210	arg1	column					1212:1217	the as-prepared mixed-mode column	1185:1217	the as-prepared mixed-mode column	1185:1217	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	1	64	theme	3-n-octadecyl-1-vinylimidazolium	284:315	arg1	bromide					317:323	3-n-octadecyl-1-vinylimidazolium bromide	284:323	3-n-octadecyl-1-vinylimidazolium bromide	284:323	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	64	theme	3-n-octadecyl-1-vinylimidazolium	284:315	arg1	1-allylimidazolium					332:349	1-allylimidazolium	332:349	1-allylimidazolium	332:349	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	3	65	theme	hydrophobic	743:753	arg1	compounds					778:786	hydrophobic, hydrophilic and ionic compounds	743:786	hydrophobic, hydrophilic and ionic compounds	743:786	The mixed-mode retention performance was researched by hydrophobic, hydrophilic and ionic compounds.					
33189957	7	66	theme	experimental	1154:1165	arg1	results					1167:1173	The experimental results	1150:1173	The experimental results	1150:1173	The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities.					
33189957	2	67	theme	nuclear	474:480	arg1	resonance					491:499	nuclear magnetic resonance	474:499	nuclear magnetic resonance analysis	474:508	After identifications of intermediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure.					
33189957	1	68	theme	bromide	317:323	arg1	tosylate					365:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate	284:372	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	1	68	theme	bromide	317:323	arg1	monomers					388:395	functional monomers	377:395	functional monomers	377:395	A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers.					
33189957	6	69	theme	ketoprofen	1086:1095	arg1	enantioseparation					1051:1067	Fast enantioseparation	1046:1067	Fast enantioseparation of ibuprofen and ketoprofen	1046:1095	Fast enantioseparation of ibuprofen and ketoprofen were obtained within 4 min under polar organic mode.					
33189957	5	70	theme	warfarin	970:977	arg1	enantiomers					934:944	the enantiomers	930:944	the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide	930:995	Moreover, the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide were separated rapidly under reverse-phase mode.					
33599084	5	0	contain	possesses	906:914	arg2	biocompatibility					929:944	an excellent biocompatibility	916:944	an excellent biocompatibility	916:944	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	5	0	contain	possesses	906:914	arg1	hydrogel					897:904	the CS-CGO composite hydrogel	876:904	the CS-CGO composite hydrogel	876:904	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	6	1	dep	92.2	1205:1208	arg1	to					1202:1203	to	1202:1203	to	1202:1203	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	3	2	dep	hydrogel	391:398	arg1	comparison					357:366	comparison	357:366	comparison	357:366	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	4	3	theme	CGO	746:748	arg1	increases					758:766	the CGO loading increases	742:766	the CGO loading increases	742:766	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	4	3	theme	CGO	746:748	arg1	desirable					778:786	desirable	778:786	desirable	778:786	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	6	4	from	wounds	1055:1060	arg1	rat					1078:1080	experimental rat	1065:1080	experimental rat	1065:1080	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	2	5	theme	enhanced	300:307	arg1	interaction					309:319	the enhanced interaction	296:319	the enhanced interaction between chitosan and CGO sheets	296:351	The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets.					
33599084	6	6	theme	wound	1149:1153	arg1	healing					1155:1161	wound healing	1149:1161	wound healing	1149:1161	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	3	7	theme	CS-GO	375:379	arg1	hydrogel					391:398	the CS-GO composite hydrogel	371:398	the CS-GO composite hydrogel	371:398	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	3	8	from	strain	513:518	arg1	4.2 MPa					502:508	4.2 MPa	502:508	4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa	502:605	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	6	9	theme	closure	1178:1184	arg1	rate					1186:1189	the wound closure rate	1168:1189	the wound closure rate	1168:1189	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	2	10	dep	chitosan	329:336	arg1	sheets					346:351	sheets	346:351	sheets	346:351	The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets.					
33599084	7	11	theme	feasible	1228:1235	arg1	strategy					1237:1244	A feasible strategy	1226:1244	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing	1226:1330	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	6	12	theme	full-thickness	1031:1044	arg1	wounds					1055:1060	full-thickness excision wounds	1031:1060	full-thickness excision wounds in experimental rat	1031:1080	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	6	13	theme	wound	1172:1176	arg1	closure					1178:1184	wound closure	1172:1184	the wound closure rate	1168:1189	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	4	14	theme	interconnected	636:649	arg1	structure					658:666	An interconnected porous structure	633:666	An interconnected porous structure	633:666	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	3	15	theme	hydrogel	448:455	arg1	stress					417:422	the compressive stress	401:422	the compressive stress of the CS-CGO composite hydrogel	401:455	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	3	16	theme	%	527:527	arg1	stress					542:547	the tensile stress	530:547	the tensile stress	530:547	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	3	16	theme	%	527:527	arg1	strain					513:518	strain	513:518	strain of 78.4%	513:527	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	3	16	theme	%	527:527	arg1	strain					553:558	strain	553:558	strain	553:558	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	6	17	theme	experimental	1065:1076	arg1	rat					1078:1080	experimental rat	1065:1080	experimental rat	1065:1080	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	0	18	theme	Graphene	15:22	arg1	Hydrogel					50:57	Graphene Oxide-Reinforced Chitosan Hydrogel	15:57	Graphene Oxide-Reinforced Chitosan Hydrogel	15:57	Functionalized Graphene Oxide-Reinforced Chitosan Hydrogel as Biomimetic Dressing for Wound Healing.					
33599084	6	19	dep	In	1009:1010	arg1	vivo					1012:1015	vivo	1012:1015	vivo	1012:1015	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	6	20	from	evaluation	1017:1026	arg1	wounds					1055:1060	full-thickness excision wounds	1031:1060	full-thickness excision wounds in experimental rat	1031:1080	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	5	21	theme	excellent	919:927	arg1	biocompatibility					929:944	an excellent biocompatibility	916:944	an excellent biocompatibility	916:944	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	3	22	theme	CS-CGO	431:436	arg1	hydrogel					448:455	the CS-CGO composite hydrogel	427:455	the CS-CGO composite hydrogel	427:455	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	4	23	theme	CS-CGO	685:690	arg1	hydrogel					702:709	the CS-CGO composite hydrogel	681:709	the CS-CGO composite hydrogel	681:709	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	1	24	theme	novel	103:107	arg1	hydrogel					128:135	A novel chitosan composite hydrogel	101:135	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO)	101:184	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	3	25	theme	composite	438:446	arg1	hydrogel					448:455	the CS-CGO composite hydrogel	427:455	the CS-CGO composite hydrogel	427:455	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	0	26	theme	Chitosan	41:48	arg1	Hydrogel					50:57	Graphene Oxide-Reinforced Chitosan Hydrogel	15:57	Graphene Oxide-Reinforced Chitosan Hydrogel	15:57	Functionalized Graphene Oxide-Reinforced Chitosan Hydrogel as Biomimetic Dressing for Wound Healing.					
33599084	3	27	theme	tensile	534:540	arg1	stress					542:547	the tensile stress	530:547	the tensile stress	530:547	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	5	28	theme	fibroblasts	996:1006	arg1	proliferation					979:991	proliferation	979:991	proliferation	979:991	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	5	28	theme	fibroblasts	996:1006	arg1	adhesion					966:973	adhesion	966:973	adhesion	966:973	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	6	29	theme	excision	1046:1053	arg1	wounds					1055:1060	full-thickness excision wounds	1031:1060	full-thickness excision wounds in experimental rat	1031:1080	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	0	30	theme	Oxide-Reinforced	24:39	arg1	Hydrogel					50:57	Graphene Oxide-Reinforced Chitosan Hydrogel	15:57	Graphene Oxide-Reinforced Chitosan Hydrogel	15:57	Functionalized Graphene Oxide-Reinforced Chitosan Hydrogel as Biomimetic Dressing for Wound Healing.					
33599084	3	31	theme	%	616:616	arg1	strain					483:488	strain	483:488	strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively	483:630	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	1	32	theme	composite	118:126	arg1	hydrogel					128:135	A novel chitosan composite hydrogel	101:135	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO)	101:184	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	5	33	theme	cytotoxicity	843:854	arg1	tests					856:860	the cytotoxicity tests	839:860	the cytotoxicity tests	839:860	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	7	34	dep	strategy	1237:1244	arg1	fabricate					1249:1257	fabricate	1249:1257	to fabricate an enhanced chitosan composite hydrogel for application in wound healing	1246:1330	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	7	35	theme	enhanced	1262:1269	arg1	hydrogel					1290:1297	an enhanced chitosan composite hydrogel	1259:1297	an enhanced chitosan composite hydrogel	1259:1297	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	7	36	theme	chitosan	1271:1278	arg1	hydrogel					1290:1297	an enhanced chitosan composite hydrogel	1259:1297	an enhanced chitosan composite hydrogel	1259:1297	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	2	37	theme	mechanical	252:261	arg1	property					263:270	the mechanical property	248:270	the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets	248:351	The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets.					
33599084	3	38	from	strain	553:558	arg1	4.2 MPa					502:508	4.2 MPa	502:508	4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa	502:605	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	5	39	dep	adhesion	966:973	arg1	the					962:964	the	962:964	the	962:964	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	7	40	theme	wound	1318:1322	arg1	healing					1324:1330	wound healing	1318:1330	wound healing	1318:1330	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	3	41	theme	composite	381:389	arg1	hydrogel					391:398	the CS-GO composite hydrogel	371:398	the CS-GO composite hydrogel	371:398	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	4	42	theme	pore	719:722	arg1	size					724:727	the pore size	715:727	the pore size	715:727	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	3	43	theme	compressive	405:415	arg1	stress					417:422	the compressive stress	401:422	the compressive stress of the CS-CGO composite hydrogel	401:455	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	4	44	theme	mechanical	805:814	arg1	property					816:823	its mechanical property	801:823	its mechanical property	801:823	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	3	45	theme	%	497:497	arg1	strain					483:488	strain	483:488	strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively	483:630	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	0	46	theme	Biomimetic	62:71	arg1	Dressing					73:80	Biomimetic Dressing	62:80	Biomimetic Dressing for Wound Healing	62:98	Functionalized Graphene Oxide-Reinforced Chitosan Hydrogel as Biomimetic Dressing for Wound Healing.					
33599084	2	47	theme	CGO	221:223	arg1	introduction					205:216	The introduction	201:216	The introduction of CGO	201:223	The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets.					
33599084	5	48	theme	CS-CGO	880:885	arg1	hydrogel					897:904	the CS-CGO composite hydrogel	876:904	the CS-CGO composite hydrogel	876:904	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	4	49	theme	loading	750:756	arg1	increases					758:766	the CGO loading increases	742:766	the CGO loading increases	742:766	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	4	49	theme	loading	750:756	arg1	desirable					778:786	desirable	778:786	desirable	778:786	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	2	50	theme	hydrogel	278:285	arg1	property					263:270	the mechanical property	248:270	the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets	248:351	The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets.					
33599084	6	51	theme	In	1009:1010	arg1	evaluation					1017:1026	In vivo evaluation	1009:1026	In vivo evaluation on full-thickness excision wounds in experimental rat	1009:1080	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	4	52	theme	composite	692:700	arg1	hydrogel					702:709	the CS-CGO composite hydrogel	681:709	the CS-CGO composite hydrogel	681:709	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
33599084	1	53	theme	chitosan	109:116	arg1	hydrogel					128:135	A novel chitosan composite hydrogel	101:135	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO)	101:184	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	6	54	theme	composite	1104:1112	arg1	hydrogel					1114:1121	the CS-CGO composite hydrogel	1093:1121	the CS-CGO composite hydrogel	1093:1121	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	2	55	theme	CS	275:276	arg1	hydrogel					278:285	CS hydrogel	275:285	CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets	275:351	The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets.					
33599084	7	56	from	application	1303:1313	arg1	healing					1324:1330	wound healing	1318:1330	wound healing	1318:1330	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	1	57	theme	functionalized	150:163	arg1	oxide					174:178	functionalized graphene oxide	150:178	functionalized graphene oxide (CGO)	150:184	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	1	57	theme	functionalized	150:163	arg1	CGO					181:183	CGO	181:183	CGO	181:183	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	5	58	theme	composite	887:895	arg1	hydrogel					897:904	the CS-CGO composite hydrogel	876:904	the CS-CGO composite hydrogel	876:904	Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts.					
33599084	0	59	theme	Wound	86:90	arg1	Healing					92:98	Wound Healing	86:98	Wound Healing	86:98	Functionalized Graphene Oxide-Reinforced Chitosan Hydrogel as Biomimetic Dressing for Wound Healing.					
33599084	7	60	theme	composite	1280:1288	arg1	hydrogel					1290:1297	an enhanced chitosan composite hydrogel	1259:1297	an enhanced chitosan composite hydrogel	1259:1297	A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.					
33599084	1	61	theme	graphene	165:172	arg1	oxide					174:178	functionalized graphene oxide	150:178	functionalized graphene oxide (CGO)	150:184	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	1	61	theme	graphene	165:172	arg1	CGO					181:183	CGO	181:183	CGO	181:183	A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated.					
33599084	3	62	from	stress	542:547	arg1	4.2 MPa					502:508	4.2 MPa	502:508	4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa	502:605	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	6	63	theme	CS-CGO	1097:1102	arg1	hydrogel					1114:1121	the CS-CGO composite hydrogel	1093:1121	the CS-CGO composite hydrogel	1093:1121	In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days.					
33599084	3	64	dep	strain	513:518	arg1	improve					560:566	improve	560:566	improve from 141.2 kPa and 134.6% to 300.2 kPa	560:605	In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively.					
33599084	4	65	theme	porous	651:656	arg1	structure					658:666	An interconnected porous structure	633:666	An interconnected porous structure	633:666	An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property.					
32747265	0	0	theme	physiological	89:101	arg1	solutions					110:118	physiological saline solutions	89:118	physiological saline solutions	89:118	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	5	1	theme	NaCl	715:718	arg1	solutions					720:728	dilute 0.15 M NaCl solutions	701:728	dilute 0.15 M NaCl solutions	701:728	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	10	2	theme	reported	1318:1325	arg1	results					1340:1346	The reported experimental results	1314:1346	The reported experimental results	1314:1346	The reported experimental results provide useful information for the design of new biomaterials starting from pullulan and globular proteins.					
32747265	5	3	theme	Na	864:865	arg1	ions					867:870	Na ions	864:870	Na ions	864:870	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	5	4	theme	dilute	701:706	arg1	solutions					720:728	dilute 0.15 M NaCl solutions	701:728	dilute 0.15 M NaCl solutions	701:728	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	2	5	theme	viscometric	411:421	arg1	behavior					423:430	the viscometric behavior	407:430	the viscometric behavior of the PULL/BSA saline solutions	407:463	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32747265	6	6	theme	large	879:883	arg1	deviations					885:894	large deviations	879:894	large deviations from ideal behavior	879:914	Thus, large deviations from ideal behavior were observed for BSA containing systems.					
32747265	1	7	theme	bovine	159:164	arg1	BSA					181:183	BSA	181:183	BSA	181:183	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	1	7	theme	bovine	159:164	arg1	albumin					172:178	bovine serum albumin	159:178	bovine serum albumin (BSA)	159:184	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	1	8	theme	isoelectric	298:308	arg1	point					310:314	the isoelectric point	294:314	the isoelectric point of BSA	294:321	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	3	9	theme	viscometric	475:485	arg1	data					487:490	the viscometric data	471:490	the viscometric data	471:490	From the viscometric data, the contributions of binary, ternary and quaternary intersegmental contacts were determined.					
32747265	1	10	theme	physiological	235:247	arg1	solution					256:263	physiological saline solution	235:263	physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA)	235:322	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	0	11	theme	saline	103:108	arg1	solutions					110:118	physiological saline solutions	89:118	physiological saline solutions	89:118	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	1	12	from	pH	278:279	arg1	solution					256:263	physiological saline solution	235:263	physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA)	235:322	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	1	13	theme	saline	249:254	arg1	solution					256:263	physiological saline solution	235:263	physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA)	235:322	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	2	14	theme	saline	448:453	arg1	solutions					455:463	the PULL/BSA saline solutions	435:463	the PULL/BSA saline solutions	435:463	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32747265	9	15	theme	phase	1282:1286	arg1	behavior					1288:1295	the phase behavior	1278:1295	the phase behavior of the mixtures	1278:1311	The results obtained by viscometry and osmometry were corroborated, allowing the prediction of the phase behavior of the mixtures.					
32747265	8	16	theme	PULL	1136:1139	arg1	chains					1141:1146	the PULL chains	1132:1146	the PULL chains through the electrolyte molecules	1132:1180	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	10	17	theme	useful	1356:1361	arg1	information					1363:1373	useful information	1356:1373	useful information for the design of new biomaterials starting from pullulan and globular proteins	1356:1453	The reported experimental results provide useful information for the design of new biomaterials starting from pullulan and globular proteins.					
32747265	1	18	theme	serum	166:170	arg1	BSA					181:183	BSA	181:183	BSA	181:183	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	1	18	theme	serum	166:170	arg1	albumin					172:178	bovine serum albumin	159:178	bovine serum albumin (BSA)	159:184	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	2	19	theme	PULL/BSA	439:446	arg1	solutions					455:463	the PULL/BSA saline solutions	435:463	the PULL/BSA saline solutions	435:463	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32747265	1	20	theme	BSA	319:321	arg1	point					310:314	the isoelectric point	294:314	the isoelectric point of BSA	294:321	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	1	21	from	°C	271:272	arg1	solution					256:263	physiological saline solution	235:263	physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA)	235:322	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	10	22	theme	biomaterials	1397:1408	arg1	design					1383:1388	the design	1379:1388	the design of new biomaterials starting from pullulan and globular proteins	1379:1453	The reported experimental results provide useful information for the design of new biomaterials starting from pullulan and globular proteins.					
32747265	1	23	theme	albumin	172:178	arg1	behavior					147:154	the behavior	143:154	the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures	143:213	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	8	24	theme	adsorption	1100:1109	arg1	complexation					1067:1078	The complexation	1063:1078	The complexation	1063:1078	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	8	24	theme	adsorption	1100:1109	arg1	molecules					1119:1127	the adsorption protein molecules	1096:1127	the adsorption protein molecules on the PULL chains through the electrolyte molecules	1096:1180	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	10	25	theme	new	1393:1395	arg1	biomaterials					1397:1408	new biomaterials	1393:1408	new biomaterials starting from pullulan and globular proteins	1393:1453	The reported experimental results provide useful information for the design of new biomaterials starting from pullulan and globular proteins.					
32747265	6	26	contain	containing	938:947	arg2	systems					949:955	systems	949:955	systems	949:955	Thus, large deviations from ideal behavior were observed for BSA containing systems.					
32747265	6	26	contain	containing	938:947	arg1	BSA					934:936	BSA	934:936	BSA containing systems	934:955	Thus, large deviations from ideal behavior were observed for BSA containing systems.					
32747265	0	27	theme	Associative	0:10	arg1	interactions					12:23	Associative interactions	0:23	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.	0:119	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	10	28	theme	globular	1437:1444	arg1	proteins					1446:1453	globular proteins	1437:1453	globular proteins	1437:1453	The reported experimental results provide useful information for the design of new biomaterials starting from pullulan and globular proteins.					
32747265	4	29	theme	composition	685:695	arg1	function					638:645	a function	636:645	a function of PULL/BSA mixture concentration and composition	636:695	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	4	29	theme	composition	685:695	arg1	measurements					603:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	8	30	theme	protein	1111:1117	arg1	complexation					1067:1078	The complexation	1063:1078	The complexation	1063:1078	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	8	30	theme	protein	1111:1117	arg1	molecules					1119:1127	the adsorption protein molecules	1096:1127	the adsorption protein molecules on the PULL chains through the electrolyte molecules	1096:1180	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	2	31	theme	Wolf	371:374	arg1	approach					376:383	The new Wolf approach	363:383	The new Wolf approach	363:383	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32747265	9	32	theme	mixtures	1304:1311	arg1	behavior					1288:1295	the phase behavior	1278:1295	the phase behavior of the mixtures	1278:1311	The results obtained by viscometry and osmometry were corroborated, allowing the prediction of the phase behavior of the mixtures.					
32747265	8	33	theme	electrolyte	1160:1170	arg1	molecules					1172:1180	the electrolyte molecules	1156:1180	the electrolyte molecules	1156:1180	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	5	34	theme	electrostatic	735:747	arg1	repulsion					749:757	the electrostatic repulsion	731:757	the electrostatic repulsion between the BSA molecules	731:783	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	2	35	theme	new	367:369	arg1	approach					376:383	The new Wolf approach	363:383	The new Wolf approach	363:383	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32747265	4	36	theme	PULL/BSA	650:657	arg1	concentration					667:679	PULL/BSA mixture concentration	650:679	PULL/BSA mixture concentration	650:679	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	4	37	theme	pressure	594:601	arg1	function					638:645	a function	636:645	a function of PULL/BSA mixture concentration and composition	636:695	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	4	37	theme	pressure	594:601	arg1	measurements					603:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	3	38	theme	ternary	522:528	arg1	contacts					560:567	binary, ternary and quaternary intersegmental contacts	514:567	binary, ternary and quaternary intersegmental contacts	514:567	From the viscometric data, the contributions of binary, ternary and quaternary intersegmental contacts were determined.					
32747265	1	39	theme	pullulan	190:197	arg1	mixtures					206:213	pullulan (PULL) mixtures	190:213	pullulan (PULL) mixtures	190:213	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	5	40	theme	attractive	811:820	arg1	interactions					835:846	attractive PULL/protein interactions	811:846	attractive PULL/protein interactions	811:846	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	7	41	theme	protein	1020:1026	arg1	content					1028:1034	the protein content	1016:1034	the protein content	1016:1034	This effect could be attributed to complexes formation as the protein content increases in the solution.					
32747265	0	42	from	albumin	78:84	arg1	solutions					110:118	physiological saline solutions	89:118	physiological saline solutions	89:118	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	5	43	theme	BSA	771:773	arg1	molecules					775:783	the BSA molecules	767:783	the BSA molecules	767:783	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	4	44	theme	Osmotic	586:592	arg1	function					638:645	a function	636:645	a function of PULL/BSA mixture concentration and composition	636:695	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	4	44	theme	Osmotic	586:592	arg1	measurements					603:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	0	45	theme	bovine	65:70	arg1	albumin					78:84	negatively charged bovine serum albumin	46:84	negatively charged bovine serum albumin	46:84	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	6	46	theme	ideal	901:905	arg1	behavior					907:914	ideal behavior	901:914	ideal behavior	901:914	Thus, large deviations from ideal behavior were observed for BSA containing systems.					
32747265	5	47	theme	M	713:713	arg1	solutions					720:728	dilute 0.15 M NaCl solutions	701:728	dilute 0.15 M NaCl solutions	701:728	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	3	48	theme	binary	514:519	arg1	contacts					560:567	binary, ternary and quaternary intersegmental contacts	514:567	binary, ternary and quaternary intersegmental contacts	514:567	From the viscometric data, the contributions of binary, ternary and quaternary intersegmental contacts were determined.					
32747265	9	49	theme	behavior	1288:1295	arg1	prediction					1264:1273	the prediction	1260:1273	the prediction of the phase behavior of the mixtures	1260:1311	The results obtained by viscometry and osmometry were corroborated, allowing the prediction of the phase behavior of the mixtures.					
32747265	4	50	theme	mixture	659:665	arg1	concentration					667:679	PULL/BSA mixture concentration	650:679	PULL/BSA mixture concentration	650:679	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	0	51	theme	charged	57:63	arg1	albumin					78:84	negatively charged bovine serum albumin	46:84	negatively charged bovine serum albumin	46:84	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	1	52	theme	=	281:281	arg1	pH					278:279	pH = 7.4	278:285	pH = 7.4	278:285	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	2	53	used	used	389:392	arg2	approach					376:383	The new Wolf approach	363:383	The new Wolf approach	363:383	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32747265	1	54	theme	membrane	343:350	arg1	osmometry					352:360	membrane osmometry	343:360	membrane osmometry	343:360	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	1	55	theme	present	128:134	arg1	study					136:140	the present study	124:140	the present study	124:140	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	6	56	from	behavior	907:914	arg1	deviations					885:894	large deviations	879:894	large deviations from ideal behavior	879:914	Thus, large deviations from ideal behavior were observed for BSA containing systems.					
32747265	3	57	theme	contacts	560:567	arg1	contributions					497:509	the contributions	493:509	the contributions of binary, ternary and quaternary intersegmental contacts	493:567	From the viscometric data, the contributions of binary, ternary and quaternary intersegmental contacts were determined.					
32747265	1	58	theme	PULL	200:203	arg1	mixtures					206:213	pullulan (PULL) mixtures	190:213	pullulan (PULL) mixtures	190:213	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	7	59	theme	complexes	993:1001	arg1	formation					1003:1011	complexes formation	993:1011	complexes formation	993:1011	This effect could be attributed to complexes formation as the protein content increases in the solution.					
32747265	4	60	theme	concentration	667:679	arg1	function					638:645	a function	636:645	a function of PULL/BSA mixture concentration and composition	636:695	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	4	60	theme	concentration	667:679	arg1	measurements					603:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements	586:614	Osmotic pressure measurements were carried out as a function of PULL/BSA mixture concentration and composition.					
32747265	5	61	theme	0.15	708:711	arg1	M					713:713	M	713:713	M	713:713	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	8	62	from	molecules	1119:1127	arg1	chains					1141:1146	the PULL chains	1132:1146	the PULL chains through the electrolyte molecules	1132:1180	The complexation was depicted as the adsorption protein molecules on the PULL chains through the electrolyte molecules.					
32747265	3	63	theme	quaternary	534:543	arg1	contacts					560:567	binary, ternary and quaternary intersegmental contacts	514:567	binary, ternary and quaternary intersegmental contacts	514:567	From the viscometric data, the contributions of binary, ternary and quaternary intersegmental contacts were determined.					
32747265	5	64	theme	PULL/protein	822:833	arg1	interactions					835:846	attractive PULL/protein interactions	811:846	attractive PULL/protein interactions	811:846	In dilute 0.15 M NaCl solutions, the electrostatic repulsion between the BSA molecules is partially screened and attractive PULL/protein interactions are mediated by Na ions.					
32747265	1	65	theme	mixtures	206:213	arg1	behavior					147:154	the behavior	143:154	the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures	143:213	In the present study, the behavior of bovine serum albumin (BSA) and pullulan (PULL) mixtures was investigated in physiological saline solution at 37 °C and pH = 7.4 (above the isoelectric point of BSA), by viscometry and membrane osmometry.					
32747265	0	66	theme	serum	72:76	arg1	albumin					78:84	negatively charged bovine serum albumin	46:84	negatively charged bovine serum albumin	46:84	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	0	67	from	pullulan	33:40	arg1	solutions					110:118	physiological saline solutions	89:118	physiological saline solutions	89:118	Associative interactions between pullulan and negatively charged bovine serum albumin in physiological saline solutions.					
32747265	3	68	theme	intersegmental	545:558	arg1	contacts					560:567	binary, ternary and quaternary intersegmental contacts	514:567	binary, ternary and quaternary intersegmental contacts	514:567	From the viscometric data, the contributions of binary, ternary and quaternary intersegmental contacts were determined.					
32747265	10	69	theme	experimental	1327:1338	arg1	results					1340:1346	The reported experimental results	1314:1346	The reported experimental results	1314:1346	The reported experimental results provide useful information for the design of new biomaterials starting from pullulan and globular proteins.					
32747265	2	70	theme	solutions	455:463	arg1	behavior					423:430	the viscometric behavior	407:430	the viscometric behavior of the PULL/BSA saline solutions	407:463	The new Wolf approach was used for modeling the viscometric behavior of the PULL/BSA saline solutions.					
32402987	0	0	theme	pretreatments	94:106	arg1	effect					18:23	the effect	14:23	the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp	14:203	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	1	1	theme	enzymatic	376:384	arg1	hydrolysis					386:395	enzymatic hydrolysis	376:395	enzymatic hydrolysis of mixed corn stalk and potato pulp silage	376:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	3	2	theme	A1	677:678	arg1	addition					658:665	the addition	654:665	the addition of strain A1 alone	654:684	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	0	3	theme	cellulase	84:92	arg1	pretreatments					94:106	feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments	28:106	pretreatments	94:106	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	4	theme	pulp	200:203	arg1	degradation					127:137	lignocellulosic degradation	111:137	lignocellulosic degradation	111:137	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	4	theme	pulp	200:203	arg1	conversion					153:162	cellulose conversion	143:162	cellulose conversion	143:162	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	2	5	theme	corn	518:521	arg1	stalk					523:527	rehydrated corn stalk	507:527	rehydrated corn stalk	507:527	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	0	6	theme	lignocellulosic	111:125	arg1	degradation					127:137	lignocellulosic degradation	111:137	lignocellulosic degradation	111:137	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	7	from	Evaluation	0:9	arg1	degradation					127:137	lignocellulosic degradation	111:137	lignocellulosic degradation	111:137	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	7	from	Evaluation	0:9	arg1	conversion					153:162	cellulose conversion	143:162	cellulose conversion	143:162	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	2	8	theme	corn	583:586	arg1	stalk					588:592	dry corn stalk	579:592	dry corn stalk	579:592	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	3	9	theme	co-ensiled	870:879	arg1	stalk					886:890	co-ensiled corn stalk	870:890	co-ensiled corn stalk	870:890	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	4	10	theme	potential	954:962	arg1	benefit					964:970	a potential benefit	952:970	a potential benefit of pretreatment for biogas production	952:1008	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	4	10	theme	potential	954:962	arg1	mixture					1038:1044	the co-ensiled HD ratio mixture	1014:1044	the co-ensiled HD ratio mixture without additive treatment	1014:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	11	theme	mixed	400:404	arg1	stalk					411:415	mixed corn stalk	400:415	mixed corn stalk	400:415	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	2	12	theme	dry	579:581	arg1	stalk					588:592	dry corn stalk	579:592	dry corn stalk	579:592	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	1	13	theme	combination	297:307	arg1	effects					210:216	The effects	206:216	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage	206:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	14	theme	corn	406:409	arg1	stalk					411:415	mixed corn stalk	400:415	mixed corn stalk	400:415	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	2	15	theme	5:11	612:615	arg1	pulp					604:607	potato pulp	597:607	potato pulp of 5:11 (LD)	597:620	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	2	16	theme	weight	563:568	arg1	ratio					570:574	a weight ratio	561:574	a weight ratio of dry corn stalk to potato pulp of 5:11 (LD)	561:620	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	2	17	theme	potato	532:537	arg1	pulp					539:542	potato pulp	532:542	potato pulp of 35:1 (HD)	532:555	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	1	18	theme	stalk	411:415	arg1	hydrolysis					386:395	enzymatic hydrolysis	376:395	enzymatic hydrolysis of mixed corn stalk and potato pulp silage	376:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	18	theme	stalk	411:415	arg1	characteristics					329:343	the fermentation characteristics	312:343	the fermentation characteristics	312:343	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	18	theme	stalk	411:415	arg1	composition					359:369	carbohydrate composition	346:369	carbohydrate composition	346:369	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	3	19	theme	strain	670:675	arg1	A1					677:678	strain A1	670:678	strain A1 alone	670:684	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	2	20	theme	rehydrated	507:516	arg1	stalk					523:527	rehydrated corn stalk	507:527	rehydrated corn stalk	507:527	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	3	21	theme	potato	896:901	arg1	pulp					903:906	potato pulp	896:906	potato pulp	896:906	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	4	22	theme	co-ensiled	1018:1027	arg1	benefit					964:970	a potential benefit	952:970	a potential benefit of pretreatment for biogas production	952:1008	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	4	22	theme	co-ensiled	1018:1027	arg1	mixture					1038:1044	the co-ensiled HD ratio mixture	1014:1044	the co-ensiled HD ratio mixture without additive treatment	1014:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	2	23	theme	potato	597:602	arg1	pulp					604:607	potato pulp	597:607	potato pulp of 5:11 (LD)	597:620	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	4	24	theme	fermentation	1144:1155	arg1	d					1139:1139	60 d	1136:1139	60 d of fermentation	1136:1155	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	2	25	theme	stalk	588:592	arg1	ratio					570:574	a weight ratio	561:574	a weight ratio of dry corn stalk to potato pulp of 5:11 (LD)	561:620	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	2	25	theme	stalk	588:592	arg1	ratio					498:502	a weight ratio	489:502	a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD)	489:555	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	0	26	theme	effect	18:23	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.	0:204	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	3	27	theme	cellulose	722:730	arg1	conversion					732:741	cellulose conversion	722:741	cellulose conversion	722:741	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	4	28	theme	enzymatic	913:921	arg1	results					934:940	The enzymatic hydrolysis results	909:940	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment	909:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	3	29	theme	lignocellulosic	794:808	arg1	degradation					810:820	the lignocellulosic degradation	790:820	the lignocellulosic degradation	790:820	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	4	30	theme	biogas	992:997	arg1	production					999:1008	biogas production	992:1008	biogas production	992:1008	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	2	31	theme	mixture	463:469	arg1	ratios					471:476	Two mixture ratios	459:476	Two mixture ratios	459:476	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	4	32	theme	ratio	1032:1036	arg1	benefit					964:970	a potential benefit	952:970	a potential benefit of pretreatment for biogas production	952:1008	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	4	32	theme	ratio	1032:1036	arg1	mixture					1038:1044	the co-ensiled HD ratio mixture	1014:1044	the co-ensiled HD ratio mixture without additive treatment	1014:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	33	theme	fermentation	316:327	arg1	characteristics					329:343	the fermentation characteristics	312:343	the fermentation characteristics	312:343	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	0	34	theme	cellulose	143:151	arg1	conversion					153:162	cellulose conversion	143:162	cellulose conversion	143:162	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	1	35	theme	potato	421:426	arg1	silage					433:438	potato pulp silage	421:438	potato pulp silage	421:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	2	36	theme	weight	491:496	arg1	ratio					498:502	a weight ratio	489:502	a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD)	489:555	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	1	37	theme	feruloyl	221:228	arg1	A1					273:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	0	38	theme	feruloyl	28:35	arg1	plantarum					70:78	feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments	28:106	plantarum	70:78	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	39	theme	co-ensiled	167:176	arg1	stalk					183:187	co-ensiled corn stalk	167:187	co-ensiled corn stalk	167:187	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	1	40	theme	pulp	428:431	arg1	silage					433:438	potato pulp silage	421:438	potato pulp silage	421:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	41	theme	esterase-producing	230:247	arg1	A1					273:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	4	42	theme	glucose	1085:1091	arg1	yield					1093:1097	high glucose yield	1080:1097	high glucose yield	1080:1097	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	4	43	theme	hydrolysis	923:932	arg1	results					934:940	The enzymatic hydrolysis results	909:940	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment	909:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	44	theme	silage	433:438	arg1	hydrolysis					386:395	enzymatic hydrolysis	376:395	enzymatic hydrolysis of mixed corn stalk and potato pulp silage	376:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	44	theme	silage	433:438	arg1	characteristics					329:343	the fermentation characteristics	312:343	the fermentation characteristics	312:343	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	44	theme	silage	433:438	arg1	composition					359:369	carbohydrate composition	346:369	carbohydrate composition	346:369	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	4	45	theme	additive	1054:1061	arg1	treatment					1063:1071	additive treatment	1054:1071	additive treatment	1054:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	46	theme	Lactobacillus	249:261	arg1	A1					273:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	0	47	theme	Lactobacillus	56:68	arg1	plantarum					70:78	feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments	28:106	plantarum	70:78	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	1	48	theme	carbohydrate	346:357	arg1	composition					359:369	carbohydrate composition	346:369	carbohydrate composition	346:369	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	0	49	theme	stalk	183:187	arg1	degradation					127:137	lignocellulosic degradation	111:137	lignocellulosic degradation	111:137	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	49	theme	stalk	183:187	arg1	conversion					153:162	cellulose conversion	143:162	cellulose conversion	143:162	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	2	50	theme	stalk	523:527	arg1	ratio					570:574	a weight ratio	561:574	a weight ratio of dry corn stalk to potato pulp of 5:11 (LD)	561:620	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	2	50	theme	stalk	523:527	arg1	ratio					498:502	a weight ratio	489:502	a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD)	489:555	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	2	51	used	used	483:486	arg2	ratios					471:476	Two mixture ratios	459:476	Two mixture ratios	459:476	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	0	52	theme	esterase-producing	37:54	arg1	plantarum					70:78	feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments	28:106	plantarum	70:78	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	53	theme	corn	178:181	arg1	stalk					183:187	co-ensiled corn stalk	167:187	co-ensiled corn stalk	167:187	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	3	54	theme	fermentable	841:851	arg1	carbohydrates					853:865	more fermentable carbohydrates	836:865	more fermentable carbohydrates	836:865	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	0	55	theme	potato	193:198	arg1	pulp					200:203	potato pulp	193:203	potato pulp	193:203	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	56	from	degradation	127:137	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.	0:204	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	3	57	theme	lignocellulosic	690:704	arg1	degradation					706:716	lignocellulosic degradation	690:716	lignocellulosic degradation	690:716	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	4	58	theme	high	1080:1083	arg1	yield					1093:1097	high glucose yield	1080:1097	high glucose yield	1080:1097	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	59	theme	plantarum	263:271	arg1	A1					273:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	feruloyl esterase-producing Lactobacillus plantarum A1	221:274	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	3	60	with	combination	754:764	arg1	cellulase					771:779	cellulase	771:779	cellulase	771:779	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	4	61	theme	enzymatic	1105:1113	arg1	hydrolysis					1115:1124	enzymatic hydrolysis	1105:1124	enzymatic hydrolysis	1105:1124	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	62	theme	A1	273:274	arg1	effects					210:216	The effects	206:216	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage	206:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	4	63	theme	pretreatment	975:986	arg1	benefit					964:970	a potential benefit	952:970	a potential benefit of pretreatment for biogas production	952:1008	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	4	63	theme	pretreatment	975:986	arg1	mixture					1038:1044	the co-ensiled HD ratio mixture	1014:1044	the co-ensiled HD ratio mixture without additive treatment	1014:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	1	64	from	effects	210:216	arg1	hydrolysis					386:395	enzymatic hydrolysis	376:395	enzymatic hydrolysis of mixed corn stalk and potato pulp silage	376:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	64	from	effects	210:216	arg1	characteristics					329:343	the fermentation characteristics	312:343	the fermentation characteristics	312:343	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	1	64	from	effects	210:216	arg1	composition					359:369	carbohydrate composition	346:369	carbohydrate composition	346:369	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32402987	2	65	theme	35:1	547:550	arg1	pulp					539:542	potato pulp	532:542	potato pulp of 35:1 (HD)	532:555	Two mixture ratios were used: a weight ratio of rehydrated corn stalk to potato pulp of 35:1 (HD) and a weight ratio of dry corn stalk to potato pulp of 5:11 (LD).					
32402987	0	66	from	conversion	153:162	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.	0:204	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	67	theme	plantarum	70:78	arg1	effect					18:23	the effect	14:23	the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp	14:203	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	68	from	effect	18:23	arg1	degradation					127:137	lignocellulosic degradation	111:137	lignocellulosic degradation	111:137	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	0	68	from	effect	18:23	arg1	conversion					153:162	cellulose conversion	143:162	cellulose conversion	143:162	Evaluation of the effect of feruloyl esterase-producing Lactobacillus plantarum and cellulase pretreatments on lignocellulosic degradation and cellulose conversion of co-ensiled corn stalk and potato pulp.					
32402987	4	69	theme	HD	1029:1030	arg1	benefit					964:970	a potential benefit	952:970	a potential benefit of pretreatment for biogas production	952:1008	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	4	69	theme	HD	1029:1030	arg1	mixture					1038:1044	the co-ensiled HD ratio mixture	1014:1044	the co-ensiled HD ratio mixture without additive treatment	1014:1071	The enzymatic hydrolysis results indicated a potential benefit of pretreatment for biogas production, as the co-ensiled HD ratio mixture without additive treatment showed high glucose yield after enzymatic hydrolysis following 60 d of fermentation.					
32402987	3	70	theme	corn	881:884	arg1	stalk					886:890	co-ensiled corn stalk	870:890	co-ensiled corn stalk	870:890	No advantage was observed with the addition of strain A1 alone for lignocellulosic degradation and cellulose conversion, while its combination with cellulase enhanced the lignocellulosic degradation and preserved more fermentable carbohydrates in co-ensiled corn stalk and potato pulp.					
32402987	1	71	theme	cellulase	277:285	arg1	effects					210:216	The effects	206:216	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage	206:438	The effects of feruloyl esterase-producing Lactobacillus plantarum A1, cellulase, or their combination on the fermentation characteristics, carbohydrate composition, and enzymatic hydrolysis of mixed corn stalk and potato pulp silage were investigated.					
32009635	4	0	theme	SEM	750:752	arg1	micrograph					754:763	SEM micrograph	750:763	SEM micrograph	750:763	FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained.					
32009635	8	1	from	hydrogels	1137:1145	arg1	release					1106:1112	The release	1102:1112	The release of diflunisal from the hydrogels	1102:1145	The release of diflunisal from the hydrogels has been studied.					
32009635	3	2	theme	freezing	717:724	arg1	cycles					701:706	freeze-thawing cycles	686:706	freeze-thawing cycles (4, 5, 6 freezing cycles)	686:732	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	2	theme	freezing	717:724	arg1	cycles					726:731	4, 5, 6 freezing cycles	709:731	4, 5, 6 freezing cycles	709:731	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	5	3	dep	capacity	833:840	arg1	the					820:822	the	820:822	the	820:822	Also, the swelling capacity and drug loading and release of diflunisal were assessed.					
32009635	8	4	theme	diflunisal	1117:1126	arg1	release					1106:1112	The release	1102:1112	The release of diflunisal from the hydrogels	1102:1145	The release of diflunisal from the hydrogels has been studied.					
32009635	7	5	theme	minor	1074:1078	arg1	temperature					1089:1099	the minor freezing temperature	1070:1099	the minor freezing temperature	1070:1099	The swelling percentage was higher at the minor freezing temperature.					
32009635	3	6	theme	freezing	637:644	arg1	temperature					646:656	the freezing temperature	633:656	the freezing temperature (-4 °C, -20 °C, -80 °C)	633:680	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	2	7	theme	polymers	486:493	arg1	kind					468:471	kind	468:471	kind	468:471	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	2	7	theme	polymers	486:493	arg1	ratio					477:481	ratio	477:481	ratio	477:481	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	2	7	theme	polymers	486:493	arg1	conditions					449:458	the freezing conditions	436:458	the freezing conditions	436:458	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	3	8	from	%	596:596	arg1	hydrogels					535:543	hydrogels	535:543	hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition	535:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	5	9	theme	drug	846:849	arg1	loading					851:857	drug loading	846:857	drug loading	846:857	Also, the swelling capacity and drug loading and release of diflunisal were assessed.					
32009635	2	10	theme	systems	285:291	arg1	applications					263:274	The applications	259:274	The applications of these systems	259:291	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	9	11	theme	diflunisal	1290:1299	arg1	release					1301:1307	the diflunisal release	1286:1307	the diflunisal release	1286:1307	All the networks maintain the drug release for 30 h and it has been observed that a simple diffusion mechanism regulates the diflunisal release according to Korsmeyer-Peppas and Higuchi models.					
32009635	0	12	theme	Diflunisal	106:115	arg1	Studies					125:131	Diflunisal Release Studies	106:131	Diflunisal Release Studies	106:131	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
32009635	6	13	theme	pore	959:962	arg1	size					964:967	the pore size	955:967	the pore size	955:967	Results from SEM micrographs and porosimetry show that the pore size decreases, while the porosity increases at lower temperatures.					
32009635	9	14	theme	drug	1195:1198	arg1	release					1200:1206	the drug release	1191:1206	the drug release for 30 h	1191:1215	All the networks maintain the drug release for 30 h and it has been observed that a simple diffusion mechanism regulates the diflunisal release according to Korsmeyer-Peppas and Higuchi models.					
32009635	6	15	from	micrographs	917:927	arg1	Results					900:906	Results	900:906	Results from SEM micrographs and porosimetry	900:943	Results from SEM micrographs and porosimetry show that the pore size decreases, while the porosity increases at lower temperatures.					
32009635	2	16	theme	drug	379:382	arg1	loading					384:390	drug loading	379:390	drug loading	379:390	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	0	17	theme	Freeze-Thawing	2:15	arg1	Method					17:22	A Freeze-Thawing Method	0:22	A Freeze-Thawing Method	0:22	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
32009635	4	18	theme	FT-IR	735:739	arg1	spectra					741:747	FT-IR spectra	735:747	FT-IR spectra	735:747	FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained.					
32009635	6	19	from	porosimetry	933:943	arg1	Results					900:906	Results	900:906	Results from SEM micrographs and porosimetry	900:943	Results from SEM micrographs and porosimetry show that the pore size decreases, while the porosity increases at lower temperatures.					
32009635	3	20	from	chitosan	550:557	arg1	hydrogels					535:543	hydrogels	535:543	hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition	535:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	9	21	theme	diffusion	1256:1264	arg1	mechanism					1266:1274	a simple diffusion mechanism	1247:1274	a simple diffusion mechanism	1247:1274	All the networks maintain the drug release for 30 h and it has been observed that a simple diffusion mechanism regulates the diflunisal release according to Korsmeyer-Peppas and Higuchi models.					
32009635	7	22	theme	swelling	1036:1043	arg1	percentage					1045:1054	The swelling percentage	1032:1054	The swelling percentage	1032:1054	The swelling percentage was higher at the minor freezing temperature.					
32009635	7	22	theme	swelling	1036:1043	arg1	higher					1060:1065	higher	1060:1065	higher	1060:1065	The swelling percentage was higher at the minor freezing temperature.					
32009635	5	23	theme	diflunisal	874:883	arg1	release					863:869	release	863:869	release	863:869	Also, the swelling capacity and drug loading and release of diflunisal were assessed.					
32009635	5	23	theme	diflunisal	874:883	arg1	capacity					833:840	swelling capacity	824:840	swelling capacity	824:840	Also, the swelling capacity and drug loading and release of diflunisal were assessed.					
32009635	5	23	theme	diflunisal	874:883	arg1	loading					851:857	drug loading	846:857	drug loading	846:857	Also, the swelling capacity and drug loading and release of diflunisal were assessed.					
32009635	2	24	dep	porosity	337:344	arg1	e.g.					331:334	e.g.	331:334	e.g.	331:334	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	3	25	dep	temperature	646:656	arg1	°C					662:663	-4 °C	659:663	-4 °C	659:663	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	25	dep	temperature	646:656	arg1	°C					670:671	-20 °C	666:671	-20 °C	666:671	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	25	dep	temperature	646:656	arg1	°C					678:679	-80 °C	674:679	-80 °C	674:679	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	0	26	theme	Release	117:123	arg1	Studies					125:131	Diflunisal Release Studies	106:131	Diflunisal Release Studies	106:131	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
32009635	6	27	theme	lower	1012:1016	arg1	temperatures					1018:1029	lower temperatures	1012:1029	lower temperatures	1012:1029	Results from SEM micrographs and porosimetry show that the pore size decreases, while the porosity increases at lower temperatures.					
32009635	3	28	from	poly	563:566	arg1	hydrogels					535:543	hydrogels	535:543	hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition	535:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	2	29	theme	release	401:407	arg1	capacity					409:416	drug release capacity	396:416	drug release capacity	396:416	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	0	30	theme	Chitosan-Poly	35:47	arg1	Hydrogels					64:72	Chitosan-Poly(vinyl alcohol) Hydrogels	35:72	Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies	35:131	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
32009635	1	31	theme	freeze-thawing	196:209	arg1	method					211:216	the freeze-thawing method	192:216	the freeze-thawing method	192:216	Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents.					
32009635	2	32	theme	drug	396:399	arg1	capacity					409:416	drug release capacity	396:416	drug release capacity	396:416	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	2	33	theme	freezing	440:447	arg1	conditions					449:458	the freezing conditions	436:458	the freezing conditions	436:458	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	3	34	theme	freeze-thawing	686:699	arg1	cycles					726:731	4, 5, 6 freezing cycles	709:731	4, 5, 6 freezing cycles	709:731	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	34	theme	freeze-thawing	686:699	arg1	cycles					701:706	freeze-thawing cycles	686:706	freeze-thawing cycles (4, 5, 6 freezing cycles)	686:732	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	35	theme	50/50	586:590	arg1	%					596:596	50/50 w/w %	586:596	50/50 w/w % of polymer composition	586:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	35	theme	50/50	586:590	arg1	composition					609:619	polymer composition	601:619	polymer composition	601:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	9	36	theme	simple	1249:1254	arg1	mechanism					1266:1274	a simple diffusion mechanism	1247:1274	a simple diffusion mechanism	1247:1274	All the networks maintain the drug release for 30 h and it has been observed that a simple diffusion mechanism regulates the diflunisal release according to Korsmeyer-Peppas and Higuchi models.					
32009635	6	37	theme	SEM	913:915	arg1	micrographs					917:927	SEM micrographs	913:927	SEM micrographs	913:927	Results from SEM micrographs and porosimetry show that the pore size decreases, while the porosity increases at lower temperatures.					
32009635	0	38	theme	vinyl	49:53	arg1	Hydrogels					64:72	Chitosan-Poly(vinyl alcohol) Hydrogels	35:72	Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies	35:131	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
32009635	3	39	theme	w/w	592:594	arg1	%					596:596	50/50 w/w %	586:596	50/50 w/w % of polymer composition	586:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	39	theme	w/w	592:594	arg1	composition					609:619	polymer composition	601:619	polymer composition	601:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	4	40	theme	porosimetry	769:779	arg1	data					781:784	porosimetry data	769:784	porosimetry data	769:784	FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained.					
32009635	7	41	theme	freezing	1080:1087	arg1	temperature					1089:1099	the minor freezing temperature	1070:1099	the minor freezing temperature	1070:1099	The swelling percentage was higher at the minor freezing temperature.					
32009635	4	42	theme	hydrogels	789:797	arg1	spectra					741:747	FT-IR spectra	735:747	FT-IR spectra	735:747	FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained.					
32009635	4	42	theme	hydrogels	789:797	arg1	micrograph					754:763	SEM micrograph	750:763	SEM micrograph	750:763	FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained.					
32009635	4	42	theme	hydrogels	789:797	arg1	data					781:784	porosimetry data	769:784	porosimetry data	769:784	FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained.					
32009635	9	43	dep	Korsmeyer-Peppas	1322:1337	arg1	models					1351:1356	models	1351:1356	models	1351:1356	All the networks maintain the drug release for 30 h and it has been observed that a simple diffusion mechanism regulates the diflunisal release according to Korsmeyer-Peppas and Higuchi models.					
32009635	5	44	theme	swelling	824:831	arg1	capacity					833:840	swelling capacity	824:840	swelling capacity	824:840	Also, the swelling capacity and drug loading and release of diflunisal were assessed.					
32009635	2	45	dep	kind	468:471	arg1	the					464:466	the	464:466	the	464:466	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	3	46	theme	polymer	601:607	arg1	composition					609:619	polymer composition	601:619	polymer composition	601:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	1	47	theme	Chitosan-poly	134:146	arg1	hydrogels					163:171	Chitosan-poly(vinyl alcohol) hydrogels	134:171	Chitosan-poly(vinyl alcohol) hydrogels	134:171	Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents.					
32009635	3	48	theme	composition	609:619	arg1	%					596:596	50/50 w/w %	586:596	50/50 w/w % of polymer composition	586:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	48	theme	composition	609:619	arg1	composition					609:619	polymer composition	601:619	polymer composition	601:619	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	49	theme	vinyl	568:572	arg1	poly					563:566	poly	563:566	poly(vinyl alcohol)	563:581	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	3	49	theme	vinyl	568:572	arg1	alcohol					574:580	vinyl alcohol	568:580	vinyl alcohol	568:580	This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C, -20 °C, -80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles).					
32009635	2	50	theme	swelling	360:367	arg1	capacity					369:376	swelling capacity	360:376	swelling capacity	360:376	The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers.					
32009635	1	51	theme	toxic	232:236	arg1	agents					251:256	toxic crosslinking agents	232:256	toxic crosslinking agents	232:256	Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents.					
32009635	0	52	theme	alcohol	55:61	arg1	Hydrogels					64:72	Chitosan-Poly(vinyl alcohol) Hydrogels	35:72	Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies	35:131	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
32009635	1	53	theme	crosslinking	238:249	arg1	agents					251:256	toxic crosslinking agents	232:256	toxic crosslinking agents	232:256	Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents.					
32009635	1	54	theme	vinyl	148:152	arg1	Chitosan-poly					134:146	Chitosan-poly	134:146	Chitosan-poly(vinyl alcohol) hydrogels	134:171	Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents.					
32009635	1	54	theme	vinyl	148:152	arg1	alcohol					154:160	vinyl alcohol	148:160	vinyl alcohol	148:160	Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents.					
32009635	0	55	theme	Crosslinking	82:93	arg1	Agents					95:100	Crosslinking Agents	82:100	Crosslinking Agents	82:100	A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies.					
34914914	7	0	theme	preservation	1199:1210	arg1	experiment					1212:1221	Cherry tomato preservation experiment	1185:1221	Cherry tomato preservation experiment	1185:1221	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	2	1	theme	CuO	331:333	arg1	ZIF-8					335:339	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	9	2	theme	fabricated	1392:1401	arg1	films					1416:1420	The easily fabricated double-layer films	1381:1420	The easily fabricated double-layer films	1381:1420	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	6	3	theme	@	1152:1152	arg1	doping					1159:1164	CuO@ZIF-8 doping	1149:1164	CuO@ZIF-8 doping	1149:1164	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	4	4	theme	antibacterial	834:846	arg1	activity					848:855	superior antibacterial activity	825:855	superior antibacterial activity	825:855	The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers.					
34914914	1	5	theme	metal	178:182	arg1	MOFs					204:207	MOFs	204:207	MOFs	204:207	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	1	5	theme	metal	178:182	arg1	frameworks					192:201	metal organic frameworks	178:201	metal organic frameworks (MOFs)	178:208	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	6	6	theme	lay-by-layer	1127:1138	arg1	casting					1140:1146	the lay-by-layer casting	1123:1146	the lay-by-layer casting	1123:1146	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	1	7	theme	frameworks	192:201	arg1	ZIF-8					157:161	ZIF-8	157:161	ZIF-8	157:161	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	1	7	theme	frameworks	192:201	arg1	subclass					166:173	a subclass	164:173	a subclass of metal organic frameworks (MOFs)	164:208	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	3	8	theme	@	706:706	arg1	nanoparticles					713:725	CuO@ZIF-8 nanoparticles	703:725	CuO@ZIF-8 nanoparticles	703:725	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	5	9	dep	%	972:972	arg1	up					961:962	up	961:962	up	961:962	The elongation at break was enhanced and up to 17.13%, about 2.4-fold that of PLA films.					
34914914	9	10	theme	biodegradable	1470:1482	arg1	packaging					1489:1497	biodegradable food packaging	1470:1497	biodegradable food packaging	1470:1497	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	4	11	theme	CuO	897:899	arg1	dispersion					883:892	the uniform dispersion	871:892	the uniform dispersion of CuO by ZIF-8 carriers	871:917	The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers.					
34914914	9	12	theme	packaging	1489:1497	arg1	field					1461:1465	the field	1457:1465	the field of biodegradable food packaging	1457:1497	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	4	13	theme	ZIF-8	904:908	arg1	carriers					910:917	ZIF-8 carriers	904:917	ZIF-8 carriers	904:917	The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers.					
34914914	6	14	theme	TiO2	1170:1173	arg1	addition					1175:1182	TiO2 addition	1170:1182	TiO2 addition	1170:1182	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	3	15	theme	-quaternary	609:619	arg1	chitosan					630:637	-quaternary ammonium chitosan	609:637	-quaternary ammonium chitosan	609:637	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	16	theme	ammonium	767:774	arg1	layer					785:789	the PVA-quaternary ammonium chitosan layer	748:789	the PVA-quaternary ammonium chitosan layer	748:789	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	7	17	theme	tomato	1192:1197	arg1	experiment					1212:1221	Cherry tomato preservation experiment	1185:1221	Cherry tomato preservation experiment	1185:1221	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	3	18	dep	acid	571:574	arg1	chitosan					630:637	-quaternary ammonium chitosan	609:637	-quaternary ammonium chitosan	609:637	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	0	19	theme	antimicrobial	99:111	arg1	NPs					129:131	novel antimicrobial agent CuO@ZIF-8 NPs	93:131	novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation	93:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	0	20	theme	CuO	119:121	arg1	NPs					129:131	novel antimicrobial agent CuO@ZIF-8 NPs	93:131	novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation	93:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	2	21	theme	direct	385:390	arg1	strategy					404:411	environmentally-friendly direct calcination strategy	360:411	environmentally-friendly direct calcination strategy	360:411	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	0	22	theme	ZIF-8	123:127	arg1	NPs					129:131	novel antimicrobial agent CuO@ZIF-8 NPs	93:131	novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation	93:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	6	23	theme	low	1039:1041	arg1	permeability					1055:1066	low water vapor permeability	1039:1066	low water vapor permeability	1039:1066	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	2	24	theme	composite	438:446	arg1	films					461:465	the composite double-layer films	434:465	the composite double-layer films for packing materials	434:487	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	0	25	theme	novel	93:97	arg1	NPs					129:131	novel antimicrobial agent CuO@ZIF-8 NPs	93:131	novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation	93:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	0	26	theme	Polylactic	0:9	arg1	chitosan					54:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan	0:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	4	27	theme	double-layer	796:807	arg1	films					809:813	The double-layer films	792:813	The double-layer films	792:813	The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers.					
34914914	0	28	theme	alcohol-quaternary	26:43	arg1	chitosan					54:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan	0:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	1	29	theme	surface	264:270	arg1	areas					272:276	its high surface areas	255:276	its high surface areas	255:276	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	7	30	theme	harmful	1280:1286	arg1	microorganisms					1288:1301	harmful microorganisms	1280:1301	harmful microorganisms	1280:1301	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	2	31	theme	packing	471:477	arg1	materials					479:487	packing materials	471:487	packing materials	471:487	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	8	32	theme	films	1374:1378	arg1	cytocompatibility					1349:1365	the cytocompatibility	1345:1365	the cytocompatibility of the films	1345:1378	MTT assay confirmed the cytocompatibility of the films.					
34914914	0	33	theme	chitosan	54:61	arg1	films					76:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	2	34	theme	agent	325:329	arg1	ZIF-8					335:339	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	1	35	theme	dispersibility	287:300	arg1	carriers					235:242	the CuO carriers	227:242	the CuO carriers because of its high surface areas and good dispersibility	227:300	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	1	35	theme	dispersibility	287:300	arg1	ZIF-8					157:161	ZIF-8	157:161	ZIF-8	157:161	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	3	36	theme	layer	662:666	arg1	method					548:553	method	548:553	method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan	548:637	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	36	theme	layer	662:666	arg1	matrix					646:651	the matrix	642:651	the matrix of outer layer and inner layer	642:682	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	2	37	theme	novel	305:309	arg1	agent					325:329	A novel antibacterial agent	303:329	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	6	38	theme	UV-barrier	1079:1088	arg1	ability					1090:1096	strong UV-barrier ability	1072:1096	strong UV-barrier ability	1072:1096	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	9	39	theme	potential	1432:1440	arg1	possibility					1442:1452	potential possibility	1432:1452	potential possibility	1432:1452	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	2	40	theme	@	334:334	arg1	ZIF-8					335:339	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	1	41	theme	CuO	231:233	arg1	carriers					235:242	the CuO carriers	227:242	the CuO carriers because of its high surface areas and good dispersibility	227:300	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	1	41	theme	CuO	231:233	arg1	ZIF-8					157:161	ZIF-8	157:161	ZIF-8	157:161	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	6	42	theme	vapor	1049:1053	arg1	permeability					1055:1066	low water vapor permeability	1039:1066	low water vapor permeability	1039:1066	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	3	43	theme	inner	672:676	arg1	layer					678:682	inner layer	672:682	inner layer	672:682	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	9	44	theme	double-layer	1403:1414	arg1	films					1416:1420	The easily fabricated double-layer films	1381:1420	The easily fabricated double-layer films	1381:1420	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	4	45	theme	uniform	875:881	arg1	dispersion					883:892	the uniform dispersion	871:892	the uniform dispersion of CuO by ZIF-8 carriers	871:917	The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers.					
34914914	6	46	theme	CuO	1149:1151	arg1	doping					1159:1164	CuO@ZIF-8 doping	1149:1164	CuO@ZIF-8 doping	1149:1164	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	3	47	theme	CuO	703:705	arg1	nanoparticles					713:725	CuO@ZIF-8 nanoparticles	703:725	CuO@ZIF-8 nanoparticles	703:725	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	4	48	theme	superior	825:832	arg1	activity					848:855	superior antibacterial activity	825:855	superior antibacterial activity	825:855	The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers.					
34914914	1	49	theme	organic	184:190	arg1	MOFs					204:207	MOFs	204:207	MOFs	204:207	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	1	49	theme	organic	184:190	arg1	frameworks					192:201	metal organic frameworks	178:201	metal organic frameworks (MOFs)	178:208	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	0	50	theme	fruit	137:141	arg1	preservation					143:154	fruit preservation	137:154	fruit preservation	137:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	3	51	theme	polylactic	560:569	arg1	acid					571:574	polylactic acid	560:574	polylactic acid (PLA)	560:580	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	51	theme	polylactic	560:569	arg1	PLA					577:579	PLA	577:579	PLA	577:579	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	5	52	dep	2.4-fold	981:988	arg1	that					990:993	that	990:993	that	990:993	The elongation at break was enhanced and up to 17.13%, about 2.4-fold that of PLA films.					
34914914	3	53	theme	ZIF-8	707:711	arg1	nanoparticles					713:725	CuO@ZIF-8 nanoparticles	703:725	CuO@ZIF-8 nanoparticles	703:725	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	54	theme	polyvinyl	586:594	arg1	PVA					605:607	PVA	605:607	PVA	605:607	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	54	theme	polyvinyl	586:594	arg1	alcohol					596:602	polyvinyl alcohol	586:602	polyvinyl alcohol (PVA)	586:608	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	55	theme	PVA-quaternary	752:765	arg1	layer					785:789	the PVA-quaternary ammonium chitosan layer	748:789	the PVA-quaternary ammonium chitosan layer	748:789	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	9	56	theme	food	1484:1487	arg1	packaging					1489:1497	biodegradable food packaging	1470:1497	biodegradable food packaging	1470:1497	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	7	57	theme	Cherry	1185:1190	arg1	experiment					1212:1221	Cherry tomato preservation experiment	1185:1221	Cherry tomato preservation experiment	1185:1221	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	2	58	theme	antibacterial	311:323	arg1	agent					325:329	A novel antibacterial agent	303:329	A novel antibacterial agent CuO@ZIF-8	303:339	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	6	59	theme	ZIF-8	1153:1157	arg1	doping					1159:1164	CuO@ZIF-8 doping	1149:1164	CuO@ZIF-8 doping	1149:1164	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	3	60	theme	chitosan	776:783	arg1	layer					785:789	the PVA-quaternary ammonium chitosan layer	748:789	the PVA-quaternary ammonium chitosan layer	748:789	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	0	61	theme	agent	113:117	arg1	NPs					129:131	novel antimicrobial agent CuO@ZIF-8 NPs	93:131	novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation	93:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	9	62	attach	presented	1422:1430	arg2	films					1416:1420	The easily fabricated double-layer films	1381:1420	The easily fabricated double-layer films	1381:1420	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	9	62	attach	presented	1422:1430	arg1	field					1461:1465	the field	1457:1465	the field of biodegradable food packaging	1457:1497	The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.					
34914914	0	63	theme	@	122:122	arg1	NPs					129:131	novel antimicrobial agent CuO@ZIF-8 NPs	93:131	novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation	93:154	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	7	64	theme	composite	1241:1249	arg1	films					1251:1255	the composite films	1237:1255	the composite films	1237:1255	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	6	65	theme	water	1043:1047	arg1	permeability					1055:1066	low water vapor permeability	1039:1066	low water vapor permeability	1039:1066	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	8	66	theme	MTT	1325:1327	arg1	assay					1329:1333	MTT assay	1325:1333	MTT assay	1325:1333	MTT assay confirmed the cytocompatibility of the films.					
34914914	0	67	theme	acid/polyvinyl	11:24	arg1	chitosan					54:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan	0:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	7	68	theme	microorganisms	1288:1301	arg1	growth					1270:1275	the growth	1266:1275	the growth of harmful microorganisms on the fruit surface	1266:1322	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	0	69	theme	ammonium	45:52	arg1	chitosan					54:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan	0:61	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	1	70	theme	high	259:262	arg1	areas					272:276	its high surface areas	255:276	its high surface areas	255:276	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	0	71	theme	double-layer	63:74	arg1	films					76:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films	0:80	Polylactic acid/polyvinyl alcohol-quaternary ammonium chitosan double-layer films doped with novel antimicrobial agent CuO@ZIF-8 NPs for fruit preservation.					
34914914	1	72	theme	areas	272:276	arg1	carriers					235:242	the CuO carriers	227:242	the CuO carriers because of its high surface areas and good dispersibility	227:300	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	1	72	theme	areas	272:276	arg1	ZIF-8					157:161	ZIF-8	157:161	ZIF-8	157:161	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	5	73	from	break	938:942	arg1	elongation					924:933	The elongation	920:933	The elongation at break	920:942	The elongation at break was enhanced and up to 17.13%, about 2.4-fold that of PLA films.					
34914914	3	74	theme	ammonium	621:628	arg1	chitosan					630:637	-quaternary ammonium chitosan	609:637	-quaternary ammonium chitosan	609:637	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	2	75	theme	calcination	392:402	arg1	strategy					404:411	environmentally-friendly direct calcination strategy	360:411	environmentally-friendly direct calcination strategy	360:411	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	3	76	with	method	548:553	arg1	acid					571:574	polylactic acid	560:574	polylactic acid (PLA)	560:580	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	76	with	method	548:553	arg1	alcohol					596:602	polyvinyl alcohol	586:602	polyvinyl alcohol (PVA)	586:608	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	76	with	method	548:553	arg1	PLA					577:579	PLA	577:579	PLA	577:579	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	76	with	method	548:553	arg1	PVA					605:607	PVA	605:607	PVA	605:607	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	1	77	theme	good	282:285	arg1	dispersibility					287:300	good dispersibility	282:300	good dispersibility	282:300	ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility.					
34914914	5	78	theme	PLA	998:1000	arg1	films					1002:1006	PLA films	998:1006	PLA films	998:1006	The elongation at break was enhanced and up to 17.13%, about 2.4-fold that of PLA films.					
34914914	7	79	from	growth	1270:1275	arg1	surface					1316:1322	the fruit surface	1306:1322	the fruit surface	1306:1322	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	2	80	theme	double-layer	448:459	arg1	films					461:465	the composite double-layer films	434:465	the composite double-layer films for packing materials	434:487	A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials.					
34914914	3	81	theme	outer	656:660	arg1	layer					662:666	outer layer	656:666	outer layer	656:666	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	82	theme	double-layer	494:505	arg1	films					507:511	The double-layer films	490:511	The double-layer films	490:511	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	6	83	theme	strong	1072:1077	arg1	ability					1090:1096	strong UV-barrier ability	1072:1096	strong UV-barrier ability	1072:1096	Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO2 addition.					
34914914	7	84	theme	fruit	1310:1314	arg1	surface					1316:1322	the fruit surface	1306:1322	the fruit surface	1306:1322	Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface.					
34914914	3	85	theme	layer	678:682	arg1	method					548:553	method	548:553	method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan	548:637	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
34914914	3	85	theme	layer	678:682	arg1	matrix					646:651	the matrix	642:651	the matrix of outer layer and inner layer	642:682	The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer.					
32677055	12	0	theme	microbial	1891:1899	arg1	flora					1901:1905	the human intestinal microbial flora	1870:1905	the human intestinal microbial flora	1870:1905	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	10	1	from	effects	1545:1551	arg1	health					1594:1599	the human intestinal health	1573:1599	the human intestinal health	1573:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	10	2	theme	human	1577:1581	arg1	health					1594:1599	the human intestinal health	1573:1599	the human intestinal health	1573:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	9	3	theme	banana	1303:1308	arg1	powder					1310:1315	banana powder	1303:1315	banana powder	1303:1315	At the same time, banana powder also increased the proportion of Lactobacillus; however, a significant difference was not observed.					
32677055	12	4	theme	banana	1847:1852	arg1	powder					1854:1859	banana powder	1847:1859	banana powder	1847:1859	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	6	5	theme	banana	801:806	arg1	powder					808:813	banana powder	801:813	banana powder	801:813	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	10	6	used	utilized	1450:1457	arg2	powder					1436:1441	banana powder	1429:1441	banana powder	1429:1441	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	10	7	theme	intestinal	1583:1592	arg1	health					1594:1599	the human intestinal health	1573:1599	the human intestinal health	1573:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	7	8	theme	gut	985:987	arg1	microbiota					989:998	the human gut microbiota	975:998	the human gut microbiota	975:998	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	13	9	from	prebiotic	2122:2130	arg1	diet					2173:2176	our daily diet	2163:2176	our daily diet	2163:2176	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	13	9	from	prebiotic	2122:2130	arg1	applications					2146:2157	production applications	2135:2157	production applications	2135:2157	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	2	10	theme	human	307:311	arg1	microbiota					317:326	the human gut microbiota	303:326	the human gut microbiota	303:326	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	10	11	from	health	1594:1599	arg1	study					1532:1536	the study	1528:1536	the study of the effects of banana powder on the human intestinal health	1528:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	10	12	theme	specific	1462:1469	arg1	bacteria					1471:1478	specific bacteria	1462:1478	specific bacteria in human intestines	1462:1498	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	6	13	theme	propionate	856:865	arg1	concentrations					829:842	the concentrations	825:842	the concentrations of acetate, propionate, and butyrate	825:879	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	7	14	theme	rRNA	1073:1076	arg1	gene					1079:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	11	15	theme	intestinal	1730:1739	arg1	community					1751:1759	the human intestinal microbial community	1720:1759	the human intestinal microbial community	1720:1759	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	0	16	theme	output	107:112	arg1	composition					55:65	the composition	51:65	the composition of human fecal microbiota and metabolic output using in vitro fermentation	51:140	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	7	17	theme	16S	1054:1056	arg1	gene					1079:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	6	18	theme	acetate	847:853	arg1	concentrations					829:842	the concentrations	825:842	the concentrations of acetate, propionate, and butyrate	825:879	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	8	19	theme	relative	1184:1191	arg1	abundance					1193:1201	the relative abundance	1180:1201	the relative abundance of Bacteroides	1180:1216	The results indicated that banana powder significantly altered bacterial diversity, increasing the relative abundance of Bacteroides, while maintaining the proportion of Bifidobacterium in the feces.					
32677055	0	20	theme	in	120:121	arg1	fermentation					129:140	in vitro fermentation	120:140	in vitro fermentation	120:140	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	11	21	theme	powder	1801:1806	arg1	products					1782:1789	the metabolized products	1766:1789	the metabolized products of banana powder	1766:1806	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	9	22	theme	significant	1376:1386	arg1	difference					1388:1397	a significant difference	1374:1397	a significant difference	1374:1397	At the same time, banana powder also increased the proportion of Lactobacillus; however, a significant difference was not observed.					
32677055	1	23	from	rich	155:158	arg1	carbohydrates					176:188	indigestible carbohydrates	163:188	indigestible carbohydrates	163:188	Bananas are rich in indigestible carbohydrates and are considered potential whole-fruit prebiotics.					
32677055	0	24	dep	in	120:121	arg1	vitro					123:127	vitro	123:127	vitro	123:127	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	11	25	theme	PRACTICAL	1602:1610	arg1	APPLICATION					1612:1622	PRACTICAL APPLICATION	1602:1622	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.	1602:1823	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	0	26	theme	banana	11:16	arg1	powder					18:23	banana powder	11:23	banana powder (Musa acuminata Colla)	11:46	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	0	26	theme	banana	11:16	arg1	Colla					41:45	Musa acuminata Colla	26:45	Musa acuminata Colla	26:45	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	13	27	theme	banana	2093:2098	arg1	powder					2100:2105	banana powder	2093:2105	banana powder	2093:2105	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	2	28	theme	0.2°	439:442	arg1	Bé					444:445	22.6 ± 0.2° Bé	432:445	22.6 ± 0.2° Bé	432:445	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	0	29	from	Effects	0:6	arg1	composition					55:65	the composition	51:65	the composition of human fecal microbiota and metabolic output using in vitro fermentation	51:140	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	13	30	theme	potential	2112:2120	arg1	prebiotic					2122:2130	a potential prebiotic	2110:2130	a potential prebiotic in production applications and our daily diet	2110:2176	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	2	31	theme	Chinese	537:543	arg1	donors					545:550	six Chinese donors	533:550	six Chinese donors	533:550	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	8	32	theme	Bifidobacterium	1255:1269	arg1	proportion					1241:1250	the proportion	1237:1250	the proportion of Bifidobacterium	1237:1269	The results indicated that banana powder significantly altered bacterial diversity, increasing the relative abundance of Bacteroides, while maintaining the proportion of Bifidobacterium in the feces.					
32677055	0	33	theme	fecal	76:80	arg1	microbiota					82:91	human fecal microbiota	70:91	human fecal microbiota	70:91	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	11	34	used	used	1672:1675	arg2	fermentation					1655:1666	in vitro batch fermentation	1640:1666	in vitro batch fermentation	1640:1666	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	4	35	theme	in	641:642	arg1	fermentation					656:667	in vitro fecal fermentation	641:667	in vitro fecal fermentation	641:667	During in vitro fecal fermentation, banana polysaccharides were gradually degraded up to approximately 80%.					
32677055	11	36	theme	microbial	1741:1749	arg1	community					1751:1759	the human intestinal microbial community	1720:1759	the human intestinal microbial community	1720:1759	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	2	37	dep	Colla	411:415	arg1	Bé					444:445	22.6 ± 0.2° Bé	432:445	22.6 ± 0.2° Bé	432:445	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	37	dep	Colla	411:415	arg1	Brix					426:429	Degrees Brix	418:429	Degrees Brix	418:429	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	38	theme	acuminata	401:409	arg1	Colla					411:415	Musa acuminata Colla	396:415	Musa acuminata Colla	396:415	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	38	theme	acuminata	401:409	arg1	banana					388:393	ripe banana	383:393	ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé)	383:446	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	4	39	theme	fecal	650:654	arg1	fermentation					656:667	in vitro fecal fermentation	641:667	in vitro fecal fermentation	641:667	During in vitro fecal fermentation, banana polysaccharides were gradually degraded up to approximately 80%.					
32677055	7	40	theme	ribosomal	1058:1066	arg1	gene					1079:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	2	41	theme	ripe	383:386	arg1	Colla					411:415	Musa acuminata Colla	396:415	Musa acuminata Colla	396:415	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	41	theme	ripe	383:386	arg1	acids					368:372	short chain fatty acids	350:372	short chain fatty acids (SCFAs)	350:380	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	41	theme	ripe	383:386	arg1	banana					388:393	ripe banana	383:393	ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé)	383:446	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	13	42	theme	daily	2167:2171	arg1	diet					2173:2176	our daily diet	2163:2176	our daily diet	2163:2176	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	11	43	theme	banana	1703:1708	arg1	powder					1710:1715	banana powder	1703:1715	banana powder	1703:1715	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	0	44	theme	acuminata	31:39	arg1	powder					18:23	banana powder	11:23	banana powder (Musa acuminata Colla)	11:46	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	0	44	theme	acuminata	31:39	arg1	Colla					41:45	Musa acuminata Colla	26:45	Musa acuminata Colla	26:45	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	10	45	from	study	1532:1536	arg1	health					1594:1599	the human intestinal health	1573:1599	the human intestinal health	1573:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	3	46	theme	polysaccharides	579:593	arg1	degradation					557:567	The degradation	553:567	The degradation of banana polysaccharides	553:593	The degradation of banana polysaccharides was observed in all six fecal samples.					
32677055	10	47	theme	powder	1563:1568	arg1	effects					1545:1551	the effects	1541:1551	the effects of banana powder on the human intestinal health	1541:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	2	48	from	China	462:466	arg1	production					336:345	the production	332:345	the production	332:345	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	49	theme	fatty	362:366	arg1	SCFAs					375:379	SCFAs	375:379	SCFAs	375:379	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	49	theme	fatty	362:366	arg1	acids					368:372	short chain fatty acids	350:372	short chain fatty acids (SCFAs)	350:380	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	49	theme	fatty	362:366	arg1	banana					388:393	ripe banana	383:393	ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé)	383:446	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	12	50	theme	beneficial	1984:1993	arg1	SCFAs					1995:1999	beneficial SCFAs	1984:1999	beneficial SCFAs (acetate, propionate, and butyrate)	1984:2035	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	50	theme	beneficial	1984:1993	arg1	propionate					2011:2020	propionate	2011:2020	propionate	2011:2020	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	50	theme	beneficial	1984:1993	arg1	butyrate					2027:2034	butyrate	2027:2034	butyrate	2027:2034	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	50	theme	beneficial	1984:1993	arg1	acetate					2002:2008	acetate	2002:2008	acetate	2002:2008	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	1	51	theme	whole-fruit	219:229	arg1	prebiotics					231:240	potential whole-fruit prebiotics	209:240	potential whole-fruit prebiotics	209:240	Bananas are rich in indigestible carbohydrates and are considered potential whole-fruit prebiotics.					
32677055	10	52	from	bacteria	1471:1478	arg1	intestines					1489:1498	human intestines	1483:1498	human intestines	1483:1498	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	2	53	theme	short	350:354	arg1	SCFAs					375:379	SCFAs	375:379	SCFAs	375:379	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	53	theme	short	350:354	arg1	acids					368:372	short chain fatty acids	350:372	short chain fatty acids (SCFAs)	350:380	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	53	theme	short	350:354	arg1	banana					388:393	ripe banana	383:393	ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé)	383:446	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	12	54	theme	intestinal	1880:1889	arg1	flora					1901:1905	the human intestinal microbial flora	1870:1905	the human intestinal microbial flora	1870:1905	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	6	55	theme	powder	808:813	arg1	addition					789:796	The addition	785:796	The addition of banana powder	785:813	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	7	56	from	Changes	964:970	arg1	microbiota					989:998	the human gut microbiota	975:998	the human gut microbiota	975:998	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	7	57	theme	human	979:983	arg1	microbiota					989:998	the human gut microbiota	975:998	the human gut microbiota	975:998	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	5	58	theme	SCFAs	760:764	arg1	production					746:755	The production	742:755	The production of SCFAs	742:764	The production of SCFAs was also measured.					
32677055	8	59	theme	banana	1112:1117	arg1	powder					1119:1124	banana powder	1112:1124	banana powder	1112:1124	The results indicated that banana powder significantly altered bacterial diversity, increasing the relative abundance of Bacteroides, while maintaining the proportion of Bifidobacterium in the feces.					
32677055	10	60	theme	human	1483:1487	arg1	intestines					1489:1498	human intestines	1483:1498	human intestines	1483:1498	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	3	61	theme	fecal	619:623	arg1	samples					625:631	all six fecal samples	611:631	all six fecal samples	611:631	The degradation of banana polysaccharides was observed in all six fecal samples.					
32677055	2	62	theme	gut	313:315	arg1	microbiota					317:326	the human gut microbiota	303:326	the human gut microbiota	303:326	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	6	63	theme	butyrate	872:879	arg1	concentrations					829:842	the concentrations	825:842	the concentrations of acetate, propionate, and butyrate	825:879	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	0	64	theme	metabolic	97:105	arg1	output					107:112	metabolic output	97:112	metabolic output	97:112	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	1	65	theme	indigestible	163:174	arg1	carbohydrates					176:188	indigestible carbohydrates	163:188	indigestible carbohydrates	163:188	Bananas are rich in indigestible carbohydrates and are considered potential whole-fruit prebiotics.					
32677055	8	66	theme	bacterial	1148:1156	arg1	diversity					1158:1166	bacterial diversity	1148:1166	bacterial diversity	1148:1166	The results indicated that banana powder significantly altered bacterial diversity, increasing the relative abundance of Bacteroides, while maintaining the proportion of Bifidobacterium in the feces.					
32677055	10	67	theme	data	1511:1514	arg1	support					1516:1522	data support	1511:1522	data support for the study of the effects of banana powder on the human intestinal health	1511:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	11	68	theme	batch	1649:1653	arg1	fermentation					1655:1666	in vitro batch fermentation	1640:1666	in vitro batch fermentation	1640:1666	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	11	69	dep	APPLICATION	1612:1622	arg1	determined					1813:1822	determined	1813:1822	were determined	1808:1822	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	11	69	dep	APPLICATION	1612:1622	arg1	used					1672:1675	used	1672:1675	was used to evaluate the effect of banana powder on the human intestinal microbial community	1668:1759	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	7	70	theme	gene	1079:1082	arg1	sequencing					1036:1045	high-throughput sequencing	1020:1045	high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene	1020:1082	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	11	71	theme	banana	1794:1799	arg1	powder					1801:1806	banana powder	1794:1806	banana powder	1794:1806	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	2	72	theme	banana-induced	258:271	arg1	changes					273:279	banana-induced changes	258:279	banana-induced changes in the composition of the human gut microbiota	258:326	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	1	73	from	carbohydrates	176:188	arg1	rich					155:158	rich	155:158	rich	155:158	Bananas are rich in indigestible carbohydrates and are considered potential whole-fruit prebiotics.					
32677055	4	74	dep	in	641:642	arg1	vitro					644:648	vitro	644:648	vitro	644:648	During in vitro fecal fermentation, banana polysaccharides were gradually degraded up to approximately 80%.					
32677055	2	75	from	changes	273:279	arg1	composition					288:298	the composition	284:298	the composition of the human gut microbiota	284:326	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	12	76	theme	Bifidobacterium	1934:1948	arg1	growth					1924:1929	the growth	1920:1929	the growth of Bifidobacterium and Bacteroides	1920:1964	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	11	77	dep	in	1640:1641	arg1	vitro					1643:1647	vitro	1643:1647	vitro	1643:1647	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	9	78	theme	Lactobacillus	1350:1362	arg1	proportion					1336:1345	the proportion	1332:1345	the proportion of Lactobacillus	1332:1362	At the same time, banana powder also increased the proportion of Lactobacillus; however, a significant difference was not observed.					
32677055	6	79	theme	acetate	905:911	arg1	production					891:900	the production	887:900	the production of acetate	887:911	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	6	79	theme	acetate	905:911	arg1	higher					919:924	higher	919:924	higher	919:924	The addition of banana powder increased the concentrations of acetate, propionate, and butyrate, with the production of acetate being higher than that of propionate and butyrate.					
32677055	8	80	theme	Bacteroides	1206:1216	arg1	abundance					1193:1201	the relative abundance	1180:1201	the relative abundance of Bacteroides	1180:1216	The results indicated that banana powder significantly altered bacterial diversity, increasing the relative abundance of Bacteroides, while maintaining the proportion of Bifidobacterium in the feces.					
32677055	13	81	theme	powder	2100:2105	arg1	use					2086:2088	the use	2082:2088	the use of banana powder as a potential prebiotic in production applications and our daily diet	2082:2176	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	2	82	theme	±	437:437	arg1	Bé					444:445	22.6 ± 0.2° Bé	432:445	22.6 ± 0.2° Bé	432:445	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	11	83	theme	in	1640:1641	arg1	fermentation					1655:1666	in vitro batch fermentation	1640:1666	in vitro batch fermentation	1640:1666	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	0	84	theme	human	70:74	arg1	microbiota					82:91	human fecal microbiota	70:91	human fecal microbiota	70:91	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	13	85	theme	production	2135:2144	arg1	applications					2146:2157	production applications	2135:2157	production applications	2135:2157	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	2	86	theme	donors	545:550	arg1	feces					524:528	the feces	520:528	the feces of six Chinese donors	520:550	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	10	87	theme	banana	1556:1561	arg1	powder					1563:1568	banana powder	1556:1568	banana powder	1556:1568	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	0	88	theme	microbiota	82:91	arg1	composition					55:65	the composition	51:65	the composition of human fecal microbiota and metabolic output using in vitro fermentation	51:140	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	12	89	theme	Bacteroides	1954:1964	arg1	growth					1924:1929	the growth	1920:1929	the growth of Bifidobacterium and Bacteroides	1920:1964	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	11	90	from	effect	1693:1698	arg1	community					1751:1759	the human intestinal microbial community	1720:1759	the human intestinal microbial community	1720:1759	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	4	91	theme	banana	670:675	arg1	polysaccharides					677:691	banana polysaccharides	670:691	banana polysaccharides	670:691	During in vitro fecal fermentation, banana polysaccharides were gradually degraded up to approximately 80%.					
32677055	3	92	located	observed	599:606	arg1	samples					625:631	all six fecal samples	611:631	all six fecal samples	611:631	The degradation of banana polysaccharides was observed in all six fecal samples.					
32677055	3	92	located	observed	599:606	arg2	degradation					557:567	The degradation	553:567	The degradation of banana polysaccharides	553:593	The degradation of banana polysaccharides was observed in all six fecal samples.					
32677055	7	93	theme	RNA	1068:1070	arg1	gene					1079:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	the 16S ribosomal RNA (rRNA) gene	1050:1082	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	11	94	theme	human	1724:1728	arg1	community					1751:1759	the human intestinal microbial community	1720:1759	the human intestinal microbial community	1720:1759	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	2	95	theme	acids	368:372	arg1	production					336:345	the production	332:345	the production	332:345	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	0	96	theme	powder	18:23	arg1	Effects					0:6	Effects	0:6	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.	0:141	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	2	97	theme	Musa	396:399	arg1	Colla					411:415	Musa acuminata Colla	396:415	Musa acuminata Colla	396:415	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	97	theme	Musa	396:399	arg1	banana					388:393	ripe banana	383:393	ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé)	383:446	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	10	98	theme	effects	1545:1551	arg1	study					1532:1536	the study	1528:1536	the study of the effects of banana powder on the human intestinal health	1528:1599	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	0	99	theme	Musa	26:29	arg1	powder					18:23	banana powder	11:23	banana powder (Musa acuminata Colla)	11:46	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	0	99	theme	Musa	26:29	arg1	Colla					41:45	Musa acuminata Colla	26:45	Musa acuminata Colla	26:45	Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation.					
32677055	9	100	theme	same	1292:1295	arg1	time					1297:1300	the same time	1288:1300	the same time	1288:1300	At the same time, banana powder also increased the proportion of Lactobacillus; however, a significant difference was not observed.					
32677055	11	101	theme	metabolized	1770:1780	arg1	products					1782:1789	the metabolized products	1766:1789	the metabolized products of banana powder	1766:1806	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	7	102	theme	high-throughput	1020:1034	arg1	sequencing					1036:1045	high-throughput sequencing	1020:1045	high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene	1020:1082	Changes in the human gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene.					
32677055	2	103	theme	Degrees	418:424	arg1	Brix					426:429	Degrees Brix	418:429	Degrees Brix	418:429	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	3	104	theme	banana	572:577	arg1	polysaccharides					579:593	banana polysaccharides	572:593	banana polysaccharides	572:593	The degradation of banana polysaccharides was observed in all six fecal samples.					
32677055	11	105	theme	powder	1710:1715	arg1	effect					1693:1698	the effect	1689:1698	the effect of banana powder on the human intestinal microbial community	1689:1759	PRACTICAL APPLICATION: In this study, in vitro batch fermentation was used to evaluate the effect of banana powder on the human intestinal microbial community, and the metabolized products of banana powder were determined.					
32677055	13	106	theme	theoretical	2060:2070	arg1	basis					2072:2076	a theoretical basis	2058:2076	a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet	2058:2176	This study provided a theoretical basis for the use of banana powder as a potential prebiotic in production applications and our daily diet.					
32677055	2	107	theme	microbiota	317:326	arg1	composition					288:298	the composition	284:298	the composition of the human gut microbiota	284:326	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	1	108	theme	potential	209:217	arg1	prebiotics					231:240	potential whole-fruit prebiotics	209:240	potential whole-fruit prebiotics	209:240	Bananas are rich in indigestible carbohydrates and are considered potential whole-fruit prebiotics.					
32677055	12	109	theme	human	1874:1878	arg1	flora					1901:1905	the human intestinal microbial flora	1870:1905	the human intestinal microbial flora	1870:1905	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	110	dep	SCFAs	1995:1999	arg1	SCFAs					1995:1999	beneficial SCFAs	1984:1999	beneficial SCFAs (acetate, propionate, and butyrate)	1984:2035	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	110	dep	SCFAs	1995:1999	arg1	propionate					2011:2020	propionate	2011:2020	propionate	2011:2020	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	110	dep	SCFAs	1995:1999	arg1	butyrate					2027:2034	butyrate	2027:2034	butyrate	2027:2034	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	12	110	dep	SCFAs	1995:1999	arg1	acetate					2002:2008	acetate	2002:2008	acetate	2002:2008	Our study showed that banana powder improved the human intestinal microbial flora and promoted the growth of Bifidobacterium and Bacteroides and could produce beneficial SCFAs (acetate, propionate, and butyrate).					
32677055	10	111	theme	banana	1429:1434	arg1	powder					1436:1441	banana powder	1429:1441	banana powder	1429:1441	In summary, banana powder can be utilized by specific bacteria in human intestines, providing data support for the study of the effects of banana powder on the human intestinal health.					
32677055	2	112	theme	chain	356:360	arg1	SCFAs					375:379	SCFAs	375:379	SCFAs	375:379	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	112	theme	chain	356:360	arg1	acids					368:372	short chain fatty acids	350:372	short chain fatty acids (SCFAs)	350:380	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
32677055	2	112	theme	chain	356:360	arg1	banana					388:393	ripe banana	383:393	ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé)	383:446	To investigate banana-induced changes in the composition of the human gut microbiota and the production of short chain fatty acids (SCFAs), ripe banana (Musa acuminata Colla, Degrees Brix: 22.6 ± 0.2° Bé), from Hainan, China, was powdered and fermented in vitro for 24 hr with the feces of six Chinese donors.					
34954418	14	0	theme	bottom-up	2620:2628	arg1	approach					2630:2637	a bottom-up approach	2618:2637	a bottom-up approach with mechanical and biochemical cues	2618:2674	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	8	1	theme	calcium-phosphate	1713:1729	arg1	accumulation					1731:1742	calcium-phosphate accumulation	1713:1742	calcium-phosphate accumulation	1713:1742	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	2	2	theme	long-term	386:394	arg1	function					407:414	long-term mechanical function	386:414	long-term mechanical function	386:414	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	12	3	from	ligament-to-bone	2257:2272	arg1	transfer					2235:2242	proper transfer	2228:2242	proper transfer of load from ligament-to-bone	2228:2272	Entheses are necessary for proper transfer of load from ligament-to-bone, but currently are not restored with healing or replacements.					
34954418	14	4	with	approach	2630:2637	arg1	cues					2671:2674	mechanical and biochemical cues	2644:2674	mechanical and biochemical cues	2644:2674	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	6	5	theme	unique	1297:1302	arg1	zones					1304:1308	3 unique zones	1295:1308	3 unique zones of collagen organization	1295:1333	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	10	6	theme	SIGNIFICANCE	2048:2059	arg1	STATEMENT					2035:2043	STATEMENT	2035:2043	STATEMENT OF SIGNIFICANCE: Ligaments	2035:2070	STATEMENT OF SIGNIFICANCE: Ligaments are attached to bone via entheses.					
34954418	11	7	theme	complex	2120:2126	arg1	Entheses					2107:2114	Entheses	2107:2114	Entheses	2107:2114	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	11	7	theme	complex	2120:2126	arg1	tissues					2128:2134	complex tissues	2120:2134	complex tissues with gradients in organization, composition, and cell phenotype	2120:2198	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	7	8	contain	had	1469:1471	arg1	constructs					1458:1467	these constructs	1452:1467	these constructs	1452:1467	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	8	contain	had	1469:1471	arg2	entheses					1529:1536	immature bovine entheses	1513:1536	immature bovine entheses	1513:1536	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	8	contain	had	1469:1471	arg2	composition					1498:1508	composition	1498:1508	composition	1498:1508	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	8	contain	had	1469:1471	arg2	organization					1481:1492	similar organization	1473:1492	similar organization	1473:1492	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	1	9	theme	Fibrocartilaginous	134:151	arg1	tissues					187:193	structurally complex tissues	166:193	structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype	166:334	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	1	9	theme	Fibrocartilaginous	134:151	arg1	entheses					153:160	Fibrocartilaginous entheses	134:160	Fibrocartilaginous entheses	134:160	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	7	10	theme	immature	1513:1520	arg1	composition					1498:1508	composition	1498:1508	composition	1498:1508	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	10	theme	immature	1513:1520	arg1	organization					1481:1492	similar organization	1473:1492	similar organization	1473:1492	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	10	theme	immature	1513:1520	arg1	entheses					1529:1536	immature bovine entheses	1513:1536	immature bovine entheses	1513:1536	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	10	11	theme	STATEMENT	2035:2043	arg1	Ligaments					2062:2070	STATEMENT OF SIGNIFICANCE: Ligaments	2035:2070	STATEMENT OF SIGNIFICANCE: Ligaments	2035:2070	STATEMENT OF SIGNIFICANCE: Ligaments are attached to bone via entheses.					
34954418	15	12	theme	engineered	2945:2954	arg1	replacements					2956:2967	engineered replacements	2945:2967	engineered replacements	2945:2967	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	3	13	theme	ligament	583:590	arg1	fibroblasts					592:602	ligament fibroblasts	583:602	ligament fibroblasts	583:602	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	5	14	theme	mechanical	955:964	arg1	conditions					975:984	compressive mechanical boundary conditions	943:984	compressive mechanical boundary conditions	943:984	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	12	15	theme	load	2247:2250	arg1	transfer					2235:2242	proper transfer	2228:2242	proper transfer of load from ligament-to-bone	2228:2272	Entheses are necessary for proper transfer of load from ligament-to-bone, but currently are not restored with healing or replacements.					
34954418	6	16	theme	glycosaminoglycans	1361:1378	arg1	zones					1304:1308	3 unique zones	1295:1308	3 unique zones of collagen organization	1295:1333	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	6	16	theme	glycosaminoglycans	1361:1378	arg1	accumulation					1345:1356	zonal accumulation	1339:1356	zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen	1339:1415	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	3	17	theme	collagen	636:643	arg1	fibers					645:650	aligned native-sized collagen fibers	615:650	aligned native-sized collagen fibers using high-density collagen gels	615:683	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	5	18	theme	conditions	975:984	arg1	effect					933:938	the effect	929:938	the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses	929:1123	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	9	19	theme	culture	1806:1812	arg1	system					1814:1819	This culture system	1801:1819	This culture system	1801:1819	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	3	20	theme	aligned	615:621	arg1	fibers					645:650	aligned native-sized collagen fibers	615:650	aligned native-sized collagen fibers using high-density collagen gels	615:683	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	1	21	theme	stiff	241:245	arg1	bone					247:250	stiff bone	241:250	stiff bone	241:250	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	13	22	theme	tensile-compressive	2374:2392	arg1	conditions					2403:2412	tensile-compressive boundary conditions	2374:2412	tensile-compressive boundary conditions	2374:2412	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	3	23	theme	collagen	671:678	arg1	gels					680:683	high-density collagen gels	658:683	high-density collagen gels	658:683	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	5	24	from	effect	933:938	arg1	development					1078:1088	the development	1074:1088	the development of zonal ligament-to-bone entheses	1074:1123	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	13	25	theme	zonal	2429:2433	arg1	gradients					2435:2443	zonal gradients	2429:2443	zonal gradients in collagen organization, mineralization, and matrix composition	2429:2508	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	6	26	theme	zonal	1339:1343	arg1	accumulation					1345:1356	zonal accumulation	1339:1356	zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen	1339:1415	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	6	27	theme	X	1406:1406	arg1	glycosaminoglycans					1361:1378	glycosaminoglycans	1361:1378	glycosaminoglycans (GAGs)	1361:1385	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	6	27	theme	X	1406:1406	arg1	collagen					1408:1415	type X collagen	1401:1415	type X collagen	1401:1415	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	13	28	theme	collagen	2448:2455	arg1	organization					2457:2468	collagen organization	2448:2468	collagen organization	2448:2468	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	5	29	theme	phosphate	1022:1030	arg1	addition					994:1001	the addition	990:1001	the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent,	990:1069	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	5	29	theme	phosphate	1022:1030	arg1	conditions					975:984	compressive mechanical boundary conditions	943:984	compressive mechanical boundary conditions	943:984	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	9	30	theme	most	1842:1845	arg1	entheses					1857:1864	the most organized entheses	1838:1864	the most organized entheses	1838:1864	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	6	31	theme	organization	1322:1333	arg1	zones					1304:1308	3 unique zones	1295:1308	3 unique zones of collagen organization	1295:1333	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	6	31	theme	organization	1322:1333	arg1	accumulation					1345:1356	zonal accumulation	1339:1356	zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen	1339:1415	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	3	32	theme	boundary	700:707	arg1	conditions					709:718	mechanical boundary conditions	689:718	mechanical boundary conditions	689:718	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	13	33	theme	new	2353:2355	arg1	insight					2357:2363	new insight	2353:2363	new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments	2353:2576	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	14	34	theme	postnatal	2732:2740	arg1	development					2751:2761	postnatal enthesis development	2732:2761	postnatal enthesis development	2732:2761	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	11	35	theme	cell	2185:2188	arg1	phenotype					2190:2198	cell phenotype	2185:2198	cell phenotype	2185:2198	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	4	36	theme	ligament-to-bone	794:809	arg1	attachments					811:821	functional ligament-to-bone attachments	783:821	functional ligament-to-bone attachments	783:821	These constructs are promising ligament replacements, however functional ligament-to-bone attachments, or entheses, are required for long-term function in vivo.					
34954418	0	37	theme	boundary	86:93	arg1	conditions					95:104	mechanical boundary conditions	75:104	mechanical boundary conditions	75:104	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	7	38	theme	culture	1444:1450	arg1	weeks					1435:1439	6 weeks	1433:1439	6 weeks of culture	1433:1450	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	13	39	theme	immature	2540:2547	arg1	attachments					2566:2576	immature ligament-to-bone attachments	2540:2576	immature ligament-to-bone attachments	2540:2576	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	9	40	theme	early	1893:1897	arg1	development					1918:1928	early postnatal enthesis development	1893:1928	early postnatal enthesis development	1893:1928	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	2	41	theme	soft	437:440	arg1	healing					457:463	soft tissue-to-bone healing	437:463	soft tissue-to-bone healing	437:463	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	9	42	theme	enthesis	1909:1916	arg1	development					1918:1928	early postnatal enthesis development	1893:1928	early postnatal enthesis development	1893:1928	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	9	43	dep	produced	1821:1828	arg1	mirroring					1883:1891	mirroring	1883:1891	mirroring early postnatal enthesis development	1883:1928	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	9	43	dep	produced	1821:1828	arg1	provides					1935:1942	provides	1935:1942	provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo	1935:2032	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	14	44	theme	culture	2598:2604	arg1	system					2606:2611	this culture system	2593:2611	this culture system	2593:2611	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	5	45	theme	study	904:908	arg1	objective					886:894	The objective	882:894	The objective of this study	882:908	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	13	46	theme	similar	2529:2535	arg1	tissues					2521:2527	tissues	2521:2527	tissues similar to immature ligament-to-bone attachments	2521:2576	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	8	47	theme	increased	1641:1649	arg1	moduli					1659:1664	significantly increased tensile moduli	1627:1664	significantly increased tensile moduli	1627:1664	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	13	48	theme	matrix	2491:2496	arg1	composition					2498:2508	matrix composition	2491:2508	matrix composition	2491:2508	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	2	49	theme	engineered	468:477	arg1	replacements					479:490	engineered replacements	468:490	engineered replacements	468:490	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	0	50	theme	zonal	20:24	arg1	formation					35:43	native-like zonal enthesis formation	8:43	native-like zonal enthesis formation	8:43	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	1	51	theme	cell	321:324	arg1	phenotype					326:334	cell phenotype	321:334	cell phenotype	321:334	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	15	52	theme	enthesis	2893:2900	arg1	regeneration					2902:2913	enthesis regeneration	2893:2913	enthesis regeneration	2893:2913	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	14	53	theme	engineered	2687:2696	arg1	replacements					2698:2709	engineered replacements	2687:2709	engineered replacements which closely mirror postnatal enthesis development	2687:2761	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	5	54	theme	known	1042:1046	arg1	agent					1064:1068	a known osteoconductive agent	1040:1068	a known osteoconductive agent	1040:1068	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	5	54	theme	known	1042:1046	arg1	phosphate					1022:1030	beta-tricalcium phosphate	1006:1030	beta-tricalcium phosphate (βTCP)	1006:1037	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	6	55	theme	compressive	1140:1150	arg1	clamps					1161:1166	compressive boundary clamps	1140:1166	compressive boundary clamps	1140:1166	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	13	56	theme	ligament-to-bone	2549:2564	arg1	attachments					2566:2576	immature ligament-to-bone attachments	2540:2576	immature ligament-to-bone attachments	2540:2576	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	5	57	theme	entheses	1116:1123	arg1	development					1078:1088	the development	1074:1088	the development of zonal ligament-to-bone entheses	1074:1123	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	2	58	theme	failure	514:520	arg1	rates					522:526	high failure rates	509:526	high failure rates	509:526	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	0	59	theme	engineered	48:57	arg1	ligaments					59:67	engineered ligaments	48:67	engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate	48:131	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	14	60	theme	biochemical	2659:2669	arg1	cues					2671:2674	mechanical and biochemical cues	2644:2674	mechanical and biochemical cues	2644:2674	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	6	61	theme	tensile-compressive	1224:1242	arg1	environment					1244:1254	a zonal tensile-compressive environment	1216:1254	a zonal tensile-compressive environment	1216:1254	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	8	62	theme	GAG	1677:1679	arg1	accumulation					1681:1692	zonal GAG accumulation	1671:1692	zonal GAG accumulation	1671:1692	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	1	63	theme	complex	256:262	arg1	gradients					270:278	complex zonal gradients	256:278	complex zonal gradients in the organization, mineralization, and cell phenotype	256:334	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	14	64	theme	mechanical	2644:2653	arg1	cues					2671:2674	mechanical and biochemical cues	2644:2674	mechanical and biochemical cues	2644:2674	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	11	65	from	gradients	2141:2149	arg1	composition					2168:2178	composition	2168:2178	composition	2168:2178	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	11	65	from	gradients	2141:2149	arg1	organization					2154:2165	organization	2154:2165	organization	2154:2165	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	11	65	from	gradients	2141:2149	arg1	phenotype					2190:2198	cell phenotype	2185:2198	cell phenotype	2185:2198	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	10	66	attach	attached	2076:2083	arg1	bone					2088:2091	bone	2088:2091	bone	2088:2091	STATEMENT OF SIGNIFICANCE: Ligaments are attached to bone via entheses.					
34954418	10	66	attach	attached	2076:2083	arg2	Ligaments					2062:2070	STATEMENT OF SIGNIFICANCE: Ligaments	2035:2070	STATEMENT OF SIGNIFICANCE: Ligaments	2035:2070	STATEMENT OF SIGNIFICANCE: Ligaments are attached to bone via entheses.					
34954418	9	67	theme	in	1947:1948	arg1	platform					1956:1963	an in vitro platform	1944:1963	an in vitro platform to better understand the cues that drive enthesis maturation in vivo	1944:2032	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	5	68	theme	zonal	1093:1097	arg1	entheses					1116:1123	zonal ligament-to-bone entheses	1093:1123	zonal ligament-to-bone entheses	1093:1123	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	2	69	theme	mechanical	396:405	arg1	function					407:414	long-term mechanical function	386:414	long-term mechanical function	386:414	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	8	70	theme	endochondral	1774:1785	arg1	ossification					1787:1798	endochondral ossification	1774:1798	endochondral ossification	1774:1798	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	6	71	theme	cellular	1183:1190	arg1	contraction					1192:1202	cellular contraction	1183:1202	cellular contraction	1183:1202	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	9	72	theme	enthesis	2006:2013	arg1	maturation					2015:2024	enthesis maturation	2006:2024	enthesis maturation	2006:2024	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	9	73	dep	in	1947:1948	arg1	vitro					1950:1954	vitro	1950:1954	vitro	1950:1954	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	1	74	theme	elastic	220:226	arg1	ligaments					228:236	elastic ligaments	220:236	elastic ligaments to stiff bone	220:250	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	5	75	theme	compressive	943:953	arg1	conditions					975:984	compressive mechanical boundary conditions	943:984	compressive mechanical boundary conditions	943:984	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	7	76	theme	bovine	1522:1527	arg1	composition					1498:1508	composition	1498:1508	composition	1498:1508	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	76	theme	bovine	1522:1527	arg1	organization					1481:1492	similar organization	1473:1492	similar organization	1473:1492	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	76	theme	bovine	1522:1527	arg1	entheses					1529:1536	immature bovine entheses	1513:1536	immature bovine entheses	1513:1536	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	12	77	theme	proper	2228:2233	arg1	transfer					2235:2242	proper transfer	2228:2242	proper transfer of load from ligament-to-bone	2228:2272	Entheses are necessary for proper transfer of load from ligament-to-bone, but currently are not restored with healing or replacements.					
34954418	3	78	theme	native-sized	623:634	arg1	fibers					645:650	aligned native-sized collagen fibers	615:650	aligned native-sized collagen fibers using high-density collagen gels	615:683	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	15	79	theme	promising	2789:2797	arg1	system					2777:2782	This culture system	2764:2782	This culture system	2764:2782	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	15	79	theme	promising	2789:2797	arg1	platform					2799:2806	a promising platform	2787:2806	a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements	2787:2967	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	5	80	theme	boundary	966:973	arg1	conditions					975:984	compressive mechanical boundary conditions	943:984	compressive mechanical boundary conditions	943:984	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	6	81	theme	ligament	1263:1270	arg1	fibroblasts					1272:1282	ligament fibroblasts	1263:1282	ligament fibroblasts	1263:1282	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	4	82	theme	ligament	752:759	arg1	replacements					761:772	ligament replacements	752:772	ligament replacements	752:772	These constructs are promising ligament replacements, however functional ligament-to-bone attachments, or entheses, are required for long-term function in vivo.					
34954418	15	83	theme	graft	2925:2929	arg1	repair					2931:2936	graft repair	2925:2936	graft repair	2925:2936	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	3	84	theme	high-density	658:669	arg1	gels					680:683	high-density collagen gels	658:683	high-density collagen gels	658:683	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	15	85	theme	culture	2769:2775	arg1	system					2777:2782	This culture system	2764:2782	This culture system	2764:2782	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	15	85	theme	culture	2769:2775	arg1	platform					2799:2806	a promising platform	2787:2806	a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements	2787:2967	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	7	86	theme	similar	1473:1479	arg1	composition					1498:1508	composition	1498:1508	composition	1498:1508	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	86	theme	similar	1473:1479	arg1	organization					1481:1492	similar organization	1473:1492	similar organization	1473:1492	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	7	86	theme	similar	1473:1479	arg1	entheses					1529:1536	immature bovine entheses	1513:1536	immature bovine entheses	1513:1536	Ultimately, by 6 weeks of culture these constructs had similar organization and composition as immature bovine entheses.					
34954418	1	87	from	gradients	270:278	arg1	organization					287:298	the organization	283:298	the organization	283:298	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	1	87	from	gradients	270:278	arg1	phenotype					326:334	cell phenotype	321:334	cell phenotype	321:334	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	1	87	from	gradients	270:278	arg1	mineralization					301:314	mineralization	301:314	mineralization	301:314	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	5	88	theme	beta-tricalcium	1006:1020	arg1	phosphate					1022:1030	beta-tricalcium phosphate	1006:1030	beta-tricalcium phosphate (βTCP)	1006:1037	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	5	88	theme	beta-tricalcium	1006:1020	arg1	agent					1064:1068	a known osteoconductive agent	1040:1068	a known osteoconductive agent	1040:1068	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	5	88	theme	beta-tricalcium	1006:1020	arg1	βTCP					1033:1036	βTCP	1033:1036	βTCP	1033:1036	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	6	89	theme	type	1401:1404	arg1	glycosaminoglycans					1361:1378	glycosaminoglycans	1361:1378	glycosaminoglycans (GAGs)	1361:1385	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	6	89	theme	type	1401:1404	arg1	collagen					1408:1415	type X collagen	1401:1415	type X collagen	1401:1415	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	3	90	theme	mechanical	689:698	arg1	conditions					709:718	mechanical boundary conditions	689:718	mechanical boundary conditions	689:718	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	13	91	theme	boundary	2394:2401	arg1	conditions					2403:2412	tensile-compressive boundary conditions	2374:2412	tensile-compressive boundary conditions	2374:2412	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	9	92	theme	organized	1847:1855	arg1	entheses					1857:1864	the most organized entheses	1838:1864	the most organized entheses	1838:1864	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	6	93	theme	collagen	1313:1320	arg1	organization					1322:1333	collagen organization	1313:1333	collagen organization	1313:1333	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	0	94	theme	mechanical	75:84	arg1	conditions					95:104	mechanical boundary conditions	75:104	mechanical boundary conditions	75:104	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	1	95	from	ligaments	228:236	arg1	load					210:213	load	210:213	load from elastic ligaments to stiff bone	210:250	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	1	96	theme	complex	179:185	arg1	tissues					187:193	structurally complex tissues	166:193	structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype	166:334	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	1	96	theme	complex	179:185	arg1	entheses					153:160	Fibrocartilaginous entheses	134:160	Fibrocartilaginous entheses	134:160	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	14	97	theme	enthesis	2742:2749	arg1	development					2751:2761	postnatal enthesis development	2732:2761	postnatal enthesis development	2732:2761	Collectively, this culture system uses a bottom-up approach with mechanical and biochemical cues to produce engineered replacements which closely mirror postnatal enthesis development.					
34954418	5	98	theme	addition	994:1001	arg1	effect					933:938	the effect	929:938	the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses	929:1123	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	8	99	theme	entheses	1606:1613	arg1	maturation					1586:1595	maturation	1586:1595	maturation	1586:1595	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	4	100	theme	functional	783:792	arg1	attachments					811:821	functional ligament-to-bone attachments	783:821	functional ligament-to-bone attachments	783:821	These constructs are promising ligament replacements, however functional ligament-to-bone attachments, or entheses, are required for long-term function in vivo.					
34954418	0	101	theme	β-tricalcium	110:121	arg1	phosphate					123:131	β-tricalcium phosphate	110:131	β-tricalcium phosphate	110:131	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	5	102	theme	osteoconductive	1048:1062	arg1	agent					1064:1068	a known osteoconductive agent	1040:1068	a known osteoconductive agent	1040:1068	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	5	102	theme	osteoconductive	1048:1062	arg1	phosphate					1022:1030	beta-tricalcium phosphate	1006:1030	beta-tricalcium phosphate (βTCP)	1006:1037	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	4	103	theme	long-term	854:862	arg1	function					864:871	long-term function	854:871	long-term function	854:871	These constructs are promising ligament replacements, however functional ligament-to-bone attachments, or entheses, are required for long-term function in vivo.					
34954418	9	104	theme	postnatal	1899:1907	arg1	development					1918:1928	early postnatal enthesis development	1893:1928	early postnatal enthesis development	1893:1928	This culture system produced some of the most organized entheses to date, closely mirroring early postnatal enthesis development, and provides an in vitro platform to better understand the cues that drive enthesis maturation in vivo.					
34954418	0	105	theme	native-like	8:18	arg1	formation					35:43	native-like zonal enthesis formation	8:43	native-like zonal enthesis formation	8:43	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	8	106	theme	tensile	1651:1657	arg1	moduli					1659:1664	significantly increased tensile moduli	1627:1664	significantly increased tensile moduli	1627:1664	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	0	107	theme	enthesis	26:33	arg1	formation					35:43	native-like zonal enthesis formation	8:43	native-like zonal enthesis formation	8:43	Driving native-like zonal enthesis formation in engineered ligaments using mechanical boundary conditions and β-tricalcium phosphate.					
34954418	6	108	theme	boundary	1152:1159	arg1	clamps					1161:1166	compressive boundary clamps	1140:1166	compressive boundary clamps	1140:1166	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	5	109	theme	ligament-to-bone	1099:1114	arg1	entheses					1116:1123	zonal ligament-to-bone entheses	1093:1123	zonal ligament-to-bone entheses	1093:1123	The objective of this study was to investigate the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (βTCP), a known osteoconductive agent, on the development of zonal ligament-to-bone entheses.					
34954418	15	110	theme	enthesis	2855:2862	arg1	formation					2864:2872	enthesis formation	2855:2872	enthesis formation	2855:2872	This culture system is a promising platform to better understanding the cues that regulate enthesis formation so to better drive enthesis regeneration following graft repair and in engineered replacements.					
34954418	8	111	theme	zonal	1671:1675	arg1	accumulation					1681:1692	zonal GAG accumulation	1671:1692	zonal GAG accumulation	1671:1692	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	2	112	theme	tissue-to-bone	442:455	arg1	healing					457:463	soft tissue-to-bone healing	437:463	soft tissue-to-bone healing	437:463	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	2	113	theme	high	509:512	arg1	rates					522:526	high failure rates	509:526	high failure rates	509:526	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	3	114	theme	culture	556:562	arg1	system					564:569	a culture system	554:569	a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions	554:718	Previously, we developed a culture system that guides ligament fibroblasts to develop aligned native-sized collagen fibers using high-density collagen gels and mechanical boundary conditions.					
34954418	2	115	theme	necessary	372:380	arg1	gradients					362:370	these complex gradients	348:370	these complex gradients necessary for long-term mechanical function	348:414	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	6	116	theme	zonal	1218:1222	arg1	environment					1244:1254	a zonal tensile-compressive environment	1216:1254	a zonal tensile-compressive environment	1216:1254	We found that compressive boundary clamps, that restrict cellular contraction and produce a zonal tensile-compressive environment, guide ligament fibroblasts to produce 3 unique zones of collagen organization and zonal accumulation of glycosaminoglycans (GAGs), type II, and type X collagen.					
34954418	8	117	theme	ALP	1695:1697	arg1	activity					1699:1706	ALP activity	1695:1706	ALP activity	1695:1706	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
34954418	2	118	theme	complex	354:360	arg1	gradients					362:370	these complex gradients	348:370	these complex gradients necessary for long-term mechanical function	348:414	Currently, these complex gradients necessary for long-term mechanical function are not recreated in soft tissue-to-bone healing or engineered replacements, contributing to high failure rates.					
34954418	1	119	theme	zonal	264:268	arg1	gradients					270:278	complex zonal gradients	256:278	complex zonal gradients in the organization, mineralization, and cell phenotype	256:334	Fibrocartilaginous entheses are structurally complex tissues that translate load from elastic ligaments to stiff bone via complex zonal gradients in the organization, mineralization, and cell phenotype.					
34954418	4	120	dep	promising	742:750	arg1	required					841:848	required	841:848	are required for long-term function in vivo	837:879	These constructs are promising ligament replacements, however functional ligament-to-bone attachments, or entheses, are required for long-term function in vivo.					
34954418	13	121	from	gradients	2435:2443	arg1	composition					2498:2508	matrix composition	2491:2508	matrix composition	2491:2508	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	13	121	from	gradients	2435:2443	arg1	mineralization					2471:2484	mineralization	2471:2484	mineralization	2471:2484	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	13	121	from	gradients	2435:2443	arg1	organization					2457:2468	collagen organization	2448:2468	collagen organization	2448:2468	Here, we provide new insight into how tensile-compressive boundary conditions and βTCP drive zonal gradients in collagen organization, mineralization, and matrix composition, producing tissues similar to immature ligament-to-bone attachments.					
34954418	11	122	with	tissues	2128:2134	arg1	gradients					2141:2149	gradients	2141:2149	gradients in organization, composition, and cell phenotype	2141:2198	Entheses are complex tissues with gradients in organization, composition, and cell phenotype.					
34954418	8	123	theme	ossification	1787:1798	arg1	initiation					1760:1769	the initiation	1756:1769	the initiation of endochondral ossification	1756:1798	Further, βTCP applied under the clamp enhanced maturation of these entheses, leading to significantly increased tensile moduli, and zonal GAG accumulation, ALP activity, and calcium-phosphate accumulation, suggesting the initiation of endochondral ossification.					
33509277	6	0	theme	specific	930:937	arg1	bacteria					953:960	specific saccharolytic bacteria	930:960	specific saccharolytic bacteria (species of Bacteroides and Lactobacillus)	930:1003	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	10	1	theme	IL-17	1587:1591	arg1	levels					1577:1582	Higher serum levels	1564:1582	Higher serum levels of IL-17 and IL-6	1564:1600	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	1	2	contain	have	132:135	arg1	galacto-oligosaccharides					101:124	BACKGROUND Prebiotic galacto-oligosaccharides	80:124	BACKGROUND Prebiotic galacto-oligosaccharides (GOS)	80:130	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	1	2	contain	have	132:135	arg2	impact					176:181	an extensively demonstrated beneficial impact	137:181	an extensively demonstrated beneficial impact on intestinal health	137:202	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	1	2	contain	have	132:135	arg1	GOS					127:129	GOS	127:129	GOS	127:129	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	11	3	theme	gene	1873:1876	arg1	expression					1878:1887	significantly increased gene expression	1849:1887	significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet	1849:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	16	4	theme	microbiome	2937:2946	arg1	composition					2948:2958	microbiome composition	2937:2958	microbiome composition	2937:2958	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	12	5	theme	Colon	1970:1974	arg1	analysis					1992:1999	Colon transcriptomics analysis	1970:1999	Colon transcriptomics analysis of mice fed GOS	1970:2015	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	14	6	theme	PI3K-Akt	2464:2471	arg1	pathways					2502:2509	the PI3K-Akt and ECM-receptor interaction pathways	2460:2509	the PI3K-Akt and ECM-receptor interaction pathways	2460:2509	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	5	7	theme	Old	658:660	arg1	animals					662:668	RESULTS Old animals	650:668	RESULTS Old animals	650:668	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	3	8	theme	infection	520:528	arg1	model					486:490	a mouse model	478:490	a mouse model of Clostridioides difficile infection	478:528	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	11	9	theme	TNFα	1892:1895	arg1	expression					1878:1887	significantly increased gene expression	1849:1887	significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet	1849:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	15	10	theme	microbiota	2795:2804	arg1	functionality					2774:2786	functionality	2774:2786	functionality	2774:2786	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	15	10	theme	microbiota	2795:2804	arg1	composition					2758:2768	composition	2758:2768	composition	2758:2768	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	15	10	theme	microbiota	2795:2804	arg1	contributors					2819:2830	the main contributors	2810:2830	the main contributors to the phenotype	2810:2847	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	3	11	theme	Clostridioides	495:508	arg1	infection					520:528	Clostridioides difficile infection	495:528	Clostridioides difficile infection	495:528	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	14	12	theme	response	2446:2453	arg1	modulator					2422:2430	an important modulator	2409:2430	an important modulator of the immune response	2409:2453	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	10	13	from	groups	1730:1735	arg1	old					1775:1777	old	1775:1777	old	1775:1777	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	10	13	from	groups	1730:1735	arg1	groups					1825:1830	the control diet groups	1808:1830	the control diet groups	1808:1830	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	16	14	theme	Dietary	2862:2868	arg1	GOS					2870:2872	CONCLUSIONS Dietary GOS	2850:2872	CONCLUSIONS Dietary GOS	2850:2872	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	2	15	dep	hallmarks	261:269	arg1	dysbiosis					296:304	microbiome dysbiosis	285:304	microbiome dysbiosis	285:304	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	15	dep	hallmarks	261:269	arg1	hallmarks					261:269	hallmarks	261:269	hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut")	261:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	15	dep	hallmarks	261:269	arg1	"					363:363	"leaky gut"	353:363	"leaky gut"	353:363	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	15	dep	hallmarks	261:269	arg1	defects					344:350	intestinal barrier defects	325:350	intestinal barrier defects ("leaky gut")	325:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	15	dep	hallmarks	261:269	arg1	inflammation					307:318	inflammation	307:318	inflammation	307:318	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	6	16	theme	Lactobacillus	990:1002	arg1	species					963:969	species	963:969	species of Bacteroides and Lactobacillus	963:1002	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	2	17	theme	microbiome	285:294	arg1	dysbiosis					296:304	microbiome dysbiosis	285:304	microbiome dysbiosis	285:304	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	17	theme	microbiome	285:294	arg1	hallmarks					261:269	hallmarks	261:269	hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut")	261:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	5	18	theme	young	853:857	arg1	animals					859:865	young animals	853:865	young animals	853:865	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	6	19	theme	Bacteroides	974:984	arg1	species					963:969	species	963:969	species of Bacteroides and Lactobacillus	963:1002	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	6	20	dep	bacteria	953:960	arg1	species					963:969	species	963:969	species of Bacteroides and Lactobacillus	963:1002	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	3	21	theme	GOS	399:401	arg1	feeding					403:409	short-term GOS feeding	388:409	short-term GOS feeding	388:409	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	5	22	theme	increased	713:721	arg1	ratios					723:728	increased ratios	713:728	increased ratios of non-saccharolytic versus saccharolytic bacteria	713:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	9	23	theme	serum	1533:1537	arg1	markers					1539:1545	serum markers	1533:1545	serum markers of inflammation	1533:1561	The antibiotics were more impactful than GOS on modulating serum markers of inflammation.					
33509277	7	24	from	permeability	1239:1250	arg1	mice					1309:1312	old mice	1305:1312	old mice	1305:1312	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	10	25	theme	GOS	1726:1728	arg1	groups					1730:1735	the GOS groups	1722:1735	the GOS groups	1722:1735	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	18	26	dep	abstract	3282:3289	arg1	Video					3276:3280	Video	3276:3280	Video	3276:3280	Video abstract.					
33509277	10	27	theme	antibiotic	1648:1657	arg1	groups					1659:1664	the antibiotic groups	1644:1664	the antibiotic groups	1644:1664	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	16	28	theme	gut	2909:2911	arg1	homeostasis					2884:2894	homeostasis	2884:2894	homeostasis of the aging gut	2884:2911	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	6	29	theme	bacteria	953:960	arg1	abundance					917:925	the abundance	913:925	the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus)	913:1003	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	14	30	theme	sugar	2387:2391	arg1	moieties					2393:2400	their sugar moieties	2381:2400	their sugar moieties	2381:2400	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	12	31	theme	small-molecule	2066:2079	arg1	processes					2091:2099	small-molecule metabolic processes	2066:2099	small-molecule metabolic processes	2066:2099	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	5	32	theme	distinct	676:683	arg1	microbiome					685:694	a distinct microbiome	674:694	a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria	674:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	10	33	theme	diet	1820:1823	arg1	groups					1825:1830	the control diet groups	1808:1830	the control diet groups	1808:1830	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	13	34	theme	genes	2292:2296	arg1	expression					2262:2271	the expression	2258:2271	the expression of binding-related genes	2258:2296	In young mice, GOS induced the expression of binding-related genes.					
33509277	13	35	theme	binding-related	2276:2290	arg1	genes					2292:2296	binding-related genes	2276:2296	binding-related genes	2276:2296	In young mice, GOS induced the expression of binding-related genes.					
33509277	7	36	from	expression	1271:1280	arg1	mice					1309:1312	old mice	1305:1312	old mice	1305:1312	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	11	37	theme	old	1923:1925	arg1	mice					1927:1930	old mice	1923:1930	old mice	1923:1930	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	12	38	theme	increased	2024:2032	arg1	expression					2034:2043	increased expression	2024:2043	increased expression	2024:2043	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	5	39	theme	bacteria	772:779	arg1	ratios					723:728	increased ratios	713:728	increased ratios of non-saccharolytic versus saccharolytic bacteria	713:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	2	40	theme	GOS	248:250	arg1	diets					252:256	GOS diets	248:256	GOS diets	248:256	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	1	41	theme	beneficial	165:174	arg1	impact					176:181	an extensively demonstrated beneficial impact	137:181	an extensively demonstrated beneficial impact on intestinal health	137:202	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	0	42	from	effects	16:22	arg1	gut					75:77	the aging gut	65:77	the aging gut	65:77	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut.					
33509277	14	43	theme	galectin	2303:2310	arg1	lectin					2348:2353	a β-galactosyl-binding lectin	2325:2353	a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response	2325:2453	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	14	43	theme	galectin	2303:2310	arg1	Lgals1					2317:2322	The galectin gene Lgals1	2299:2322	The galectin gene Lgals1	2299:2322	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	17	44	theme	galectin-1	3238:3247	arg1	induction					3225:3233	the induction	3221:3233	the induction of galectin-1 in young but not old mice	3221:3273	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	7	45	theme	increased	1256:1264	arg1	expression					1271:1280	increased MUC2 expression	1256:1280	increased MUC2 expression	1256:1280	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	1	46	from	impact	176:181	arg1	health					197:202	intestinal health	186:202	intestinal health	186:202	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	17	47	theme	epithelial	3178:3187	arg1	cells					3189:3193	intestinal epithelial cells	3167:3193	intestinal epithelial cells	3167:3193	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	15	48	dep	composition	2758:2768	arg1	the					2754:2756	the	2754:2756	the	2754:2756	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	17	49	theme	young	3252:3256	arg1	mice					3270:3273	young but not old mice	3252:3273	young but not old mice	3252:3273	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	2	50	theme	aging	278:282	arg1	dysbiosis					296:304	microbiome dysbiosis	285:304	microbiome dysbiosis	285:304	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	50	theme	aging	278:282	arg1	inflammation					307:318	inflammation	307:318	inflammation	307:318	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	50	theme	aging	278:282	arg1	hallmarks					261:269	hallmarks	261:269	hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut")	261:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	12	51	from	respirasome	2122:2132	arg1	animals					2141:2147	old animals	2137:2147	old animals	2137:2147	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	11	52	theme	mice	1927:1930	arg1	tissue					1913:1918	distal colon tissue	1900:1918	distal colon tissue of old mice, which was decreased by the GOS diet	1900:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	10	53	located	observed	1607:1614	arg1	groups					1659:1664	the antibiotic groups	1644:1664	the antibiotic groups	1644:1664	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	10	53	located	observed	1607:1614	arg1	GOS					1631:1633	GOS	1631:1633	GOS	1631:1633	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	10	53	located	observed	1607:1614	arg2	levels					1577:1582	Higher serum levels	1564:1582	Higher serum levels of IL-17 and IL-6	1564:1600	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	10	53	located	observed	1607:1614	arg1	control					1619:1625	control	1619:1625	control	1619:1625	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	11	54	theme	distal	1900:1905	arg1	tissue					1913:1918	distal colon tissue	1900:1918	distal colon tissue of old mice, which was decreased by the GOS diet	1900:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	7	55	theme	increased	1218:1226	arg1	permeability					1239:1250	age-associated increased intestinal permeability	1203:1250	age-associated increased intestinal permeability	1203:1250	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	16	56	theme	intestinal	3022:3031	arg1	permeability					3033:3044	decreased intestinal permeability	3012:3044	decreased intestinal permeability	3012:3044	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	1	57	theme	BACKGROUND	80:89	arg1	galacto-oligosaccharides					101:124	BACKGROUND Prebiotic galacto-oligosaccharides	80:124	BACKGROUND Prebiotic galacto-oligosaccharides (GOS)	80:130	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	1	57	theme	BACKGROUND	80:89	arg1	GOS					127:129	GOS	127:129	GOS	127:129	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	6	58	from	abundance	1020:1028	arg1	animals					1067:1073	young and old animals	1053:1073	young and old animals	1053:1073	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	5	59	theme	saccharolytic	758:770	arg1	bacteria					772:779	non-saccharolytic versus saccharolytic bacteria	733:779	non-saccharolytic versus saccharolytic bacteria	733:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	3	60	theme	short-term	388:397	arg1	feeding					403:409	short-term GOS feeding	388:409	short-term GOS feeding	388:409	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	12	61	from	processes	2091:2099	arg1	animals					2141:2147	old animals	2137:2147	old animals	2137:2147	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	2	62	theme	diets	252:256	arg1	impact					238:243	the impact	234:243	the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut")	234:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	3	63	theme	aging	430:434	arg1	gut					436:438	the aging gut	426:438	the aging gut	426:438	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	13	64	theme	young	2234:2238	arg1	mice					2240:2243	young mice	2234:2243	young mice	2234:2243	In young mice, GOS induced the expression of binding-related genes.					
33509277	6	65	theme	overall	884:890	arg1	diversity					892:900	the overall diversity	880:900	the overall diversity	880:900	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	0	66	theme	prebiotic	27:35	arg1	galacto-oligosaccharides					37:60	prebiotic galacto-oligosaccharides	27:60	prebiotic galacto-oligosaccharides	27:60	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut.					
33509277	17	67	dep	microbiome	3138:3147	arg1	the					3134:3136	the	3134:3136	the	3134:3136	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	16	68	theme	decreased	3012:3020	arg1	permeability					3033:3044	decreased intestinal permeability	3012:3044	decreased intestinal permeability	3012:3044	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	14	69	theme	young	2532:2536	arg1	mice					2538:2541	young mice	2532:2541	young mice	2532:2541	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	0	70	theme	aging	69:73	arg1	gut					75:77	the aging gut	65:77	the aging gut	65:77	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut.					
33509277	8	71	theme	old	1464:1466	arg1	mice					1468:1471	old mice	1464:1471	old mice	1464:1471	Clyndamicin reduced the abundance Bifidobacterium while increasing Akkermansia, Clostridium, Coprococcus, Bacillus, Bacteroides, and Ruminococcus in old mice.					
33509277	3	72	theme	antibiotic	453:462	arg1	challenges					464:473	antibiotic challenges	453:473	antibiotic challenges	453:473	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	5	73	theme	lower	805:809	arg1	abundance					811:819	a lower abundance	803:819	a lower abundance of β-galactosidases	803:839	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	17	74	dep	apparent	3207:3214	arg1	factor					3100:3105	a determining factor	3086:3105	a determining factor	3086:3105	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	17	74	dep	apparent	3207:3214	arg1	Age					3078:3080	Age	3078:3080	Age	3078:3080	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	16	75	theme	host	2964:2967	arg1	expression					2974:2983	host gene expression	2964:2983	host gene expression	2964:2983	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	10	76	theme	serum	1571:1575	arg1	levels					1577:1582	Higher serum levels	1564:1582	Higher serum levels of IL-17 and IL-6	1564:1600	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	6	77	dep	robust	1134:1139	arg1	homogeneous					1142:1152	homogeneous	1142:1152	homogeneous	1142:1152	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	3	78	theme	mouse	480:484	arg1	model					486:490	a mouse model	478:490	a mouse model of Clostridioides difficile infection	478:528	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	15	79	theme	bifidogenic	2581:2591	arg1	response					2593:2600	variable bifidogenic response	2572:2600	variable bifidogenic response to GOS injected into colon organoids	2572:2637	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	6	80	theme	saccharolytic	939:951	arg1	bacteria					953:960	specific saccharolytic bacteria	930:960	specific saccharolytic bacteria (species of Bacteroides and Lactobacillus)	930:1003	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	15	81	theme	main	2814:2817	arg1	functionality					2774:2786	functionality	2774:2786	functionality	2774:2786	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	15	81	theme	main	2814:2817	arg1	composition					2758:2768	composition	2758:2768	composition	2758:2768	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	15	81	theme	main	2814:2817	arg1	contributors					2819:2830	the main contributors	2810:2830	the main contributors to the phenotype	2810:2847	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	16	82	theme	increased	3050:3058	arg1	production					3066:3075	increased mucus production	3050:3075	increased mucus production	3050:3075	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	11	83	theme	increased	1863:1871	arg1	expression					1878:1887	significantly increased gene expression	1849:1887	significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet	1849:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	7	84	theme	mucus	1286:1290	arg1	thickness					1292:1300	mucus thickness	1286:1300	mucus thickness	1286:1300	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	5	85	theme	RESULTS	650:656	arg1	animals					662:668	RESULTS Old animals	650:668	RESULTS Old animals	650:668	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	12	86	theme	oxidative	2184:2192	arg1	metabolism					2194:2203	an increased oxidative metabolism	2171:2203	an increased oxidative metabolism	2171:2203	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	3	87	theme	difficile	510:518	arg1	infection					520:528	Clostridioides difficile infection	495:528	Clostridioides difficile infection	495:528	We also evaluated if short-term GOS feeding influenced how the aging gut responded to antibiotic challenges in a mouse model of Clostridioides difficile infection.					
33509277	7	88	from	thickness	1292:1300	arg1	mice					1309:1312	old mice	1305:1312	old mice	1305:1312	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	4	89	theme	colonic	555:561	arg1	organoids					563:571	colonic organoids	555:571	colonic organoids	555:571	Finally, we assessed if colonic organoids could reproduce the GOS responder-non-responder phenotypes observed in vivo.					
33509277	2	90	theme	barrier	336:342	arg1	"					363:363	"leaky gut"	353:363	"leaky gut"	353:363	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	90	theme	barrier	336:342	arg1	defects					344:350	intestinal barrier defects	325:350	intestinal barrier defects ("leaky gut")	325:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	16	91	theme	gene	2969:2972	arg1	expression					2974:2983	host gene expression	2964:2983	host gene expression	2964:2983	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	2	92	theme	gut	274:276	arg1	aging					278:282	gut aging	274:282	gut aging	274:282	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	12	93	theme	old	2137:2139	arg1	animals					2141:2147	old animals	2137:2147	old animals	2137:2147	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	1	94	theme	intestinal	186:195	arg1	health					197:202	intestinal health	186:202	intestinal health	186:202	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	12	95	theme	transcriptomics	1976:1990	arg1	analysis					1992:1999	Colon transcriptomics analysis	1970:1999	Colon transcriptomics analysis of mice fed GOS	1970:2015	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	16	96	theme	CONCLUSIONS	2850:2860	arg1	GOS					2870:2872	CONCLUSIONS Dietary GOS	2850:2872	CONCLUSIONS Dietary GOS	2850:2872	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	17	97	theme	old	3266:3268	arg1	mice					3270:3273	young but not old mice	3252:3273	young but not old mice	3252:3273	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	15	98	theme	colon	2623:2627	arg1	organoids					2629:2637	colon organoids	2623:2637	colon organoids	2623:2637	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	2	99	theme	leaky	354:358	arg1	"					363:363	"leaky gut"	353:363	"leaky gut"	353:363	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	99	theme	leaky	354:358	arg1	defects					344:350	intestinal barrier defects	325:350	intestinal barrier defects ("leaky gut")	325:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	6	100	theme	bifidogenic	1154:1164	arg1	effect					1166:1171	a robust, homogeneous bifidogenic effect	1132:1171	a robust, homogeneous bifidogenic effect	1132:1171	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	4	101	theme	responder-non-responder	597:619	arg1	phenotypes					621:630	the GOS responder-non-responder phenotypes	589:630	the GOS responder-non-responder phenotypes observed in vivo	589:647	Finally, we assessed if colonic organoids could reproduce the GOS responder-non-responder phenotypes observed in vivo.					
33509277	16	102	theme	aging	2903:2907	arg1	gut					2909:2911	the aging gut	2899:2911	the aging gut	2899:2911	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	17	103	from	induction	3225:3233	arg1	mice					3270:3273	young but not old mice	3252:3273	young but not old mice	3252:3273	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	17	103	from	induction	3225:3233	arg1	apparent					3207:3214	apparent	3207:3214	apparent	3207:3214	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	10	104	theme	young	1791:1795	arg1	animals					1797:1803	young animals	1791:1803	young animals	1791:1803	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	14	105	theme	ECM-receptor	2477:2488	arg1	pathways					2502:2509	the PI3K-Akt and ECM-receptor interaction pathways	2460:2509	the PI3K-Akt and ECM-receptor interaction pathways	2460:2509	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	12	106	theme	genes	2048:2052	arg1	expression					2034:2043	increased expression	2024:2043	increased expression	2024:2043	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	14	107	theme	β-galactosyl-binding	2327:2346	arg1	lectin					2348:2353	a β-galactosyl-binding lectin	2325:2353	a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response	2325:2453	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	14	107	theme	β-galactosyl-binding	2327:2346	arg1	Lgals1					2317:2322	The galectin gene Lgals1	2299:2322	The galectin gene Lgals1	2299:2322	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	11	108	theme	GOS	1960:1962	arg1	diet					1964:1967	the GOS diet	1956:1967	the GOS diet	1956:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	9	109	theme	inflammation	1550:1561	arg1	markers					1539:1545	serum markers	1533:1545	serum markers of inflammation	1533:1561	The antibiotics were more impactful than GOS on modulating serum markers of inflammation.					
33509277	6	110	theme	young	1053:1057	arg1	animals					1067:1073	young and old animals	1053:1073	young and old animals	1053:1073	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	8	111	theme	abundance	1339:1347	arg1	Bifidobacterium					1349:1363	the abundance Bifidobacterium	1335:1363	the abundance Bifidobacterium	1335:1363	Clyndamicin reduced the abundance Bifidobacterium while increasing Akkermansia, Clostridium, Coprococcus, Bacillus, Bacteroides, and Ruminococcus in old mice.					
33509277	7	112	theme	old	1305:1307	arg1	mice					1309:1312	old mice	1305:1312	old mice	1305:1312	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	7	113	theme	MUC2	1266:1269	arg1	expression					1271:1280	increased MUC2 expression	1256:1280	increased MUC2 expression	1256:1280	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	10	114	theme	control	1812:1818	arg1	groups					1825:1830	the control diet groups	1808:1830	the control diet groups	1808:1830	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	1	115	theme	demonstrated	152:163	arg1	impact					176:181	an extensively demonstrated beneficial impact	137:181	an extensively demonstrated beneficial impact on intestinal health	137:202	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	6	116	theme	β-galactosidases	1033:1048	arg1	abundance					1020:1028	the abundance	1016:1028	the abundance of β-galactosidases in young and old animals	1016:1073	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	16	117	theme	mucus	3060:3064	arg1	production					3066:3075	increased mucus production	3050:3075	increased mucus production	3050:3075	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	14	118	theme	gene	2312:2315	arg1	lectin					2348:2353	a β-galactosyl-binding lectin	2325:2353	a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response	2325:2453	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	14	118	theme	gene	2312:2315	arg1	Lgals1					2317:2322	The galectin gene Lgals1	2299:2322	The galectin gene Lgals1	2299:2322	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	15	119	theme	response	2684:2691	arg1	phenotypes					2710:2719	the response and non-response phenotypes	2680:2719	phenotypes	2710:2719	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	16	120	from	changes	2926:2932	arg1	composition					2948:2958	microbiome composition	2937:2958	microbiome composition	2937:2958	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	16	120	from	changes	2926:2932	arg1	expression					2974:2983	host gene expression	2964:2983	host gene expression	2964:2983	CONCLUSIONS Dietary GOS modulated homeostasis of the aging gut by promoting changes in microbiome composition and host gene expression, which was translated into decreased intestinal permeability and increased mucus production.					
33509277	7	121	theme	intestinal	1228:1237	arg1	permeability					1239:1250	age-associated increased intestinal permeability	1203:1250	age-associated increased intestinal permeability	1203:1250	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	17	122	theme	determining	3088:3098	arg1	factor					3100:3105	a determining factor	3086:3105	a determining factor	3086:3105	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	17	122	theme	determining	3088:3098	arg1	Age					3078:3080	Age	3078:3080	Age	3078:3080	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	11	123	from	expression	1878:1887	arg1	tissue					1913:1918	distal colon tissue	1900:1918	distal colon tissue of old mice, which was decreased by the GOS diet	1900:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	10	124	dep	groups	1730:1735	arg1	higher					1712:1717	higher	1712:1717	higher	1712:1717	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	5	125	theme	non-saccharolytic	733:749	arg1	bacteria					772:779	non-saccharolytic versus saccharolytic bacteria	733:779	non-saccharolytic versus saccharolytic bacteria	733:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	12	126	theme	metabolic	2081:2089	arg1	processes					2091:2099	small-molecule metabolic processes	2066:2099	small-molecule metabolic processes	2066:2099	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	6	127	theme	non-saccharolytic	1094:1110	arg1	organisms					1112:1120	the non-saccharolytic organisms	1090:1120	the non-saccharolytic organisms	1090:1120	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	7	128	theme	age-associated	1203:1216	arg1	permeability					1239:1250	age-associated increased intestinal permeability	1203:1250	age-associated increased intestinal permeability	1203:1250	GOS reduced age-associated increased intestinal permeability and increased MUC2 expression and mucus thickness in old mice.					
33509277	0	129	theme	pleiotropic	4:14	arg1	effects					16:22	The pleiotropic effects	0:22	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut	0:77	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut.					
33509277	1	130	theme	Prebiotic	91:99	arg1	galacto-oligosaccharides					101:124	BACKGROUND Prebiotic galacto-oligosaccharides	80:124	BACKGROUND Prebiotic galacto-oligosaccharides (GOS)	80:130	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	1	130	theme	Prebiotic	91:99	arg1	GOS					127:129	GOS	127:129	GOS	127:129	BACKGROUND Prebiotic galacto-oligosaccharides (GOS) have an extensively demonstrated beneficial impact on intestinal health.					
33509277	2	131	from	impact	238:243	arg1	dysbiosis					296:304	microbiome dysbiosis	285:304	microbiome dysbiosis	285:304	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	131	from	impact	238:243	arg1	inflammation					307:318	inflammation	307:318	inflammation	307:318	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	131	from	impact	238:243	arg1	hallmarks					261:269	hallmarks	261:269	hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut")	261:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	132	theme	gut	360:362	arg1	"					363:363	"leaky gut"	353:363	"leaky gut"	353:363	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	132	theme	gut	360:362	arg1	defects					344:350	intestinal barrier defects	325:350	intestinal barrier defects ("leaky gut")	325:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	5	133	theme	β-galactosidases	824:839	arg1	abundance					811:819	a lower abundance	803:819	a lower abundance of β-galactosidases	803:839	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	5	133	theme	β-galactosidases	824:839	arg1	microbiome					685:694	a distinct microbiome	674:694	a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria	674:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	15	134	from	mice	2556:2559	arg1	Stools					2544:2549	Stools	2544:2549	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics	2544:2667	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	12	135	theme	energetic	2209:2217	arg1	efficiency					2219:2228	energetic efficiency	2209:2228	energetic efficiency	2209:2228	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	10	136	theme	IL-6	1702:1705	arg1	levels					1692:1697	levels	1692:1697	levels of IL-6	1692:1705	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	0	137	theme	galacto-oligosaccharides	37:60	arg1	effects					16:22	The pleiotropic effects	0:22	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut	0:77	The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut.					
33509277	6	138	theme	old	1063:1065	arg1	animals					1067:1073	young and old animals	1053:1073	young and old animals	1053:1073	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	15	139	theme	non-response	2697:2708	arg1	phenotypes					2710:2719	the response and non-response phenotypes	2680:2719	phenotypes	2710:2719	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	17	140	theme	intestinal	3167:3176	arg1	cells					3189:3193	intestinal epithelial cells	3167:3193	intestinal epithelial cells	3167:3193	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	14	141	theme	immune	2439:2444	arg1	response					2446:2453	the immune response	2435:2453	the immune response	2435:2453	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	15	142	theme	prebiotics	2658:2667	arg1	presence					2646:2653	the presence	2642:2653	the presence of prebiotics	2642:2667	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	10	143	dep	control	1619:1625	arg1	diets					1635:1639	diets	1635:1639	diets	1635:1639	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	5	144	contain	had	670:672	arg1	animals					662:668	RESULTS Old animals	650:668	RESULTS Old animals	650:668	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	5	144	contain	had	670:672	arg2	microbiome					685:694	a distinct microbiome	674:694	a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria	674:779	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	5	144	contain	had	670:672	arg2	abundance					811:819	a lower abundance	803:819	a lower abundance of β-galactosidases	803:839	RESULTS Old animals had a distinct microbiome characterized by increased ratios of non-saccharolytic versus saccharolytic bacteria and, correspondingly, a lower abundance of β-galactosidases compared to young animals.					
33509277	14	145	theme	interaction	2490:2500	arg1	pathways					2502:2509	the PI3K-Akt and ECM-receptor interaction pathways	2460:2509	the PI3K-Akt and ECM-receptor interaction pathways	2460:2509	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	17	146	theme	cells	3189:3193	arg1	expression					3153:3162	expression	3153:3162	expression	3153:3162	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	17	146	theme	cells	3189:3193	arg1	microbiome					3138:3147	microbiome	3138:3147	microbiome	3138:3147	Age was a determining factor on how prebiotics impacted the microbiome and expression of intestinal epithelial cells, especially apparent from the induction of galectin-1 in young but not old mice.					
33509277	12	147	theme	increased	2174:2182	arg1	metabolism					2194:2203	an increased oxidative metabolism	2171:2203	an increased oxidative metabolism	2171:2203	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	2	148	theme	intestinal	325:334	arg1	"					363:363	"leaky gut"	353:363	"leaky gut"	353:363	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	2	148	theme	intestinal	325:334	arg1	defects					344:350	intestinal barrier defects	325:350	intestinal barrier defects ("leaky gut")	325:364	In this study, we determined the impact of GOS diets on hallmarks of gut aging: microbiome dysbiosis, inflammation, and intestinal barrier defects ("leaky gut").					
33509277	6	149	theme	robust	1134:1139	arg1	effect					1166:1171	a robust, homogeneous bifidogenic effect	1132:1171	a robust, homogeneous bifidogenic effect	1132:1171	GOS reduced the overall diversity, increased the abundance of specific saccharolytic bacteria (species of Bacteroides and Lactobacillus), increased the abundance of β-galactosidases in young and old animals, and increased the non-saccharolytic organisms; however, a robust, homogeneous bifidogenic effect was not observed.					
33509277	10	150	theme	Higher	1564:1569	arg1	levels					1577:1582	Higher serum levels	1564:1582	Higher serum levels of IL-17 and IL-6	1564:1600	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
33509277	14	151	theme	important	2412:2420	arg1	modulator					2422:2430	an important modulator	2409:2430	an important modulator of the immune response	2409:2453	The galectin gene Lgals1, a β-galactosyl-binding lectin that bridges molecules by their sugar moieties and is an important modulator of the immune response, and the PI3K-Akt and ECM-receptor interaction pathways were also induced in young mice.					
33509277	15	152	theme	variable	2572:2579	arg1	response					2593:2600	variable bifidogenic response	2572:2600	variable bifidogenic response to GOS injected into colon organoids	2572:2637	Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.					
33509277	12	153	theme	mice	2004:2007	arg1	analysis					1992:1999	Colon transcriptomics analysis	1970:1999	Colon transcriptomics analysis of mice fed GOS	1970:2015	Colon transcriptomics analysis of mice fed GOS showed increased expression of genes involved in small-molecule metabolic processes and specifically the respirasome in old animals, which could indicate an increased oxidative metabolism and energetic efficiency.					
33509277	11	154	theme	colon	1907:1911	arg1	tissue					1913:1918	distal colon tissue	1900:1918	distal colon tissue of old mice, which was decreased by the GOS diet	1900:1967	RTqPCR revealed significantly increased gene expression of TNFα in distal colon tissue of old mice, which was decreased by the GOS diet.					
33509277	4	155	theme	GOS	593:595	arg1	phenotypes					621:630	the GOS responder-non-responder phenotypes	589:630	the GOS responder-non-responder phenotypes observed in vivo	589:647	Finally, we assessed if colonic organoids could reproduce the GOS responder-non-responder phenotypes observed in vivo.					
33509277	10	156	theme	IL-6	1597:1600	arg1	levels					1577:1582	Higher serum levels	1564:1582	Higher serum levels of IL-17 and IL-6	1564:1600	Higher serum levels of IL-17 and IL-6 were observed in control and GOS diets in the antibiotic groups, and within those groups, levels of IL-6 were higher in the GOS groups, regardless of age, and higher in the old compared to young animals in the control diet groups.					
32008582	7	0	contain	has	1719:1721	arg2	capacity					1748:1755	a remarkable GMP binding capacity	1723:1755	a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin	1723:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	7	0	contain	has	1719:1721	arg1	chitin					1712:1717	chitin	1712:1717	chitin	1712:1717	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	1	1	located	found	172:176	arg2	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	1	located	found	172:176	arg1	whey					188:191	cheese whey	181:191	cheese whey	181:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	1	located	found	172:176	arg2	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	1	located	found	172:176	arg2	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	1	located	found	172:176	arg2	GMP					167:169	GMP	167:169	GMP	167:169	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	5	2	theme	recovered	1045:1053	arg1	acid					1062:1065	recovered sialic acid	1045:1065	recovered sialic acid	1045:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	4	3	theme	acid	767:770	arg1	composition					772:782	amino acid composition	761:782	amino acid composition which reflects the purity of the peptide	761:823	The GMP fraction obtained from each column was analyzed for amino acid composition which reflects the purity of the peptide.					
32008582	5	4	theme	acid	1062:1065	arg1	acid					1062:1065	recovered sialic acid	1045:1065	recovered sialic acid	1045:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	4	theme	acid	1062:1065	arg1	%					1040:1040	average 78%	1030:1040	average 78% of recovered sialic acid	1030:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	5	theme	resin	1518:1522	arg1	B					1524:1524	resin B	1518:1524	resin B	1518:1524	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	6	with	B	1524:1524	arg1	difference					1534:1543	no difference	1531:1543	no difference between resin A and resin B averaging 8.7	1531:1585	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	7	7	theme	dry	1781:1783	arg1	weight					1785:1790	8.6 mg/100 mg dry weight	1767:1790	8.6 mg/100 mg dry weight of chitin	1767:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	5	8	theme	mg/100	1412:1417	arg1	weight					1426:1431	mg/100 mg dry weight	1412:1431	mg/100 mg dry weight of resin	1412:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	9	theme	chitin	644:649	arg1	B					651:651	chitin B	644:651	chitin B	644:651	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	5	10	theme	sialic	927:932	arg1	acid					934:937	sialic acid	927:937	sialic acid	927:937	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	11	dep	resin	576:580	arg1	A					589:589	resin A	583:589	resin A	583:589	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	3	11	dep	resin	576:580	arg1	B					598:598	resin B	592:598	resin B	592:598	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	3	11	dep	resin	576:580	arg1	C					610:610	resin C	604:610	resin C	604:610	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	5	12	from	C	1262:1262	arg1	fraction					1242:1249	GMP fraction	1238:1249	GMP fraction from resin C	1238:1262	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	4	13	theme	peptide	817:823	arg1	purity					803:808	the purity	799:808	the purity of the peptide	799:823	The GMP fraction obtained from each column was analyzed for amino acid composition which reflects the purity of the peptide.					
32008582	8	14	theme	Further	1856:1862	arg1	research					1864:1871	Further research	1856:1871	Further research	1856:1871	Further research is needed to develop an efficient inexpensive method to purify GMP.					
32008582	6	15	from	general	1649:1655	arg1	similar					1657:1663	similar	1657:1663	similar	1657:1663	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
32008582	7	16	theme	mg/100	1771:1776	arg1	weight					1785:1790	8.6 mg/100 mg dry weight	1767:1790	8.6 mg/100 mg dry weight of chitin	1767:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	5	17	theme	resin	1553:1557	arg1	A					1559:1559	resin A	1553:1559	resin A	1553:1559	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	18	from	chromatography	449:462	arg1	chitin					503:508	chitin	503:508	chitin	503:508	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	18	from	chromatography	449:462	arg1	resin					493:497	food grade anion exchange resin	467:497	food grade anion exchange resin	467:497	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	19	from	B	1111:1111	arg1	fraction					1080:1087	the GMP fraction	1072:1087	(2) the GMP fraction from resin A or resin B	1068:1111	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	20	theme	binding	1382:1388	arg1	capacity					1390:1397	the GMP binding capacity	1374:1397	(4) the GMP binding capacity expressed as mg/100 mg dry weight of resin	1370:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	21	dep	fraction	1242:1249	arg1	the					1234:1236	the	1234:1236	the	1234:1236	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	21	dep	fraction	1242:1249	arg1	similar					974:980	similar	974:980	similar	974:980	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	7	22	theme	binding	1740:1746	arg1	capacity					1748:1755	a remarkable GMP binding capacity	1723:1755	a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin	1723:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	3	23	theme	resin	583:587	arg1	A					589:589	resin A	583:589	resin A	583:589	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	6	24	theme	chitin	1619:1624	arg1	B					1626:1626	chitin B	1619:1626	chitin B	1619:1626	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
32008582	5	25	theme	resin	1105:1109	arg1	B					1111:1111	resin B	1105:1111	resin B	1105:1111	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	26	theme	resin	576:580	arg1	Samples					527:533	Samples	527:533	Samples of commercially available anion exchange resin (resin A, resin B and resin C)	527:611	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	1	27	theme	κ-casein	139:146	arg1	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	27	theme	κ-casein	139:146	arg1	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	27	theme	κ-casein	139:146	arg1	GMP					167:169	GMP	167:169	GMP	167:169	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	27	theme	κ-casein	139:146	arg1	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	2	28	theme	column	442:447	arg1	chromatography					449:462	column chromatography	442:462	column chromatography on food grade anion exchange resin and chitin	442:508	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	28	theme	column	442:447	arg1	adsorbent					516:524	adsorbent	516:524	adsorbent	516:524	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	29	theme	GMP	1076:1078	arg1	fraction					1080:1087	the GMP fraction	1072:1087	(2) the GMP fraction from resin A or resin B	1068:1111	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	0	30	theme	grade	77:81	arg1	adsorbent					121:129	adsorbent	121:129	adsorbent	121:129	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	30	theme	grade	77:81	arg1	resin					98:102	food grade anion exchange resin	72:102	food grade anion exchange resin	72:102	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	30	theme	grade	77:81	arg1	chitin					108:113	chitin	108:113	chitin	108:113	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	31	theme	exchange	484:491	arg1	resin					493:497	food grade anion exchange resin	467:497	food grade anion exchange resin	467:497	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	32	theme	exchange	89:96	arg1	adsorbent					121:129	adsorbent	121:129	adsorbent	121:129	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	32	theme	exchange	89:96	arg1	resin					98:102	food grade anion exchange resin	72:102	food grade anion exchange resin	72:102	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	32	theme	exchange	89:96	arg1	chitin					108:113	chitin	108:113	chitin	108:113	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	33	theme	recovered	948:956	arg1	acid					965:968	total recovered sialic acid	942:968	total recovered sialic acid	942:968	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	34	theme	anion	856:860	arg1	resin					871:875	commercial anion exchange resin	845:875	commercial anion exchange resin	845:875	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	35	theme	grade	472:476	arg1	resin					493:497	food grade anion exchange resin	467:497	food grade anion exchange resin	467:497	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	36	theme	resin	1256:1260	arg1	C					1262:1262	resin C	1256:1262	resin C	1256:1262	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	37	theme	contaminating	1145:1157	arg1	arginine					1207:1214	arginine	1207:1214	arginine	1207:1214	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	37	theme	contaminating	1145:1157	arg1	histidine					1196:1204	histidine	1196:1204	histidine	1196:1204	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	37	theme	contaminating	1145:1157	arg1	tyrosine					1220:1227	tyrosine	1220:1227	tyrosine	1220:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	37	theme	contaminating	1145:1157	arg1	phenylalanine					1181:1193	phenylalanine	1181:1193	phenylalanine	1181:1193	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	37	theme	contaminating	1145:1157	arg1	acids					1165:1169	contaminating amino acids	1145:1169	contaminating amino acids including phenylalanine, histidine, arginine and tyrosine	1145:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	38	theme	high	371:374	arg1	purity					376:381	high purity	371:381	high purity GMP	371:385	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	39	theme	whey	416:419	arg1	SWF					431:433	SWF	431:433	SWF	431:433	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	39	theme	whey	416:419	arg1	fraction					421:428	soluble whey fraction	408:428	soluble whey fraction (SWF)	408:434	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	40	theme	acids	1165:1169	arg1	level					1136:1140	undetectable level	1123:1140	undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine	1123:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	41	dep	those	617:621	arg1	A					641:641	chitin A	634:641	chitin A	634:641	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	3	41	dep	those	617:621	arg1	B					651:651	chitin B	644:651	chitin B	644:651	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	3	41	dep	those	617:621	arg1	C					664:664	chitin C	657:664	chitin C	657:664	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	2	42	attach	isolated	394:401	arg1	SWF					431:433	SWF	431:433	SWF	431:433	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	42	attach	isolated	394:401	arg2	purity					376:381	high purity	371:381	high purity GMP	371:385	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	42	attach	isolated	394:401	arg1	fraction					421:428	soluble whey fraction	408:428	soluble whey fraction (SWF)	408:434	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	43	theme	Major	826:830	arg1	findings					832:839	Major findings	826:839	Major findings for commercial anion exchange resin	826:875	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	44	theme	amino	1315:1319	arg1	acids					1321:1325	contaminating amino acids	1301:1325	contaminating amino acids	1301:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	7	45	theme	whey	1836:1839	arg1	fractionation					1841:1853	whey fractionation	1836:1853	whey fractionation	1836:1853	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	5	46	theme	mg	1419:1420	arg1	weight					1426:1431	mg/100 mg dry weight	1412:1431	mg/100 mg dry weight of resin	1412:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	0	47	theme	κ-casein	13:20	arg1	glycomacropeptide					22:38	κ-casein glycomacropeptide	13:38	κ-casein glycomacropeptide	13:38	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	7	48	theme	remarkable	1725:1734	arg1	capacity					1748:1755	a remarkable GMP binding capacity	1723:1755	a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin	1723:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	4	49	theme	GMP	705:707	arg1	fraction					709:716	The GMP fraction	701:716	The GMP fraction obtained from each column	701:742	The GMP fraction obtained from each column was analyzed for amino acid composition which reflects the purity of the peptide.					
32008582	1	50	theme	functional	282:291	arg1	food					293:296	functional food	282:296	functional food as well as dietetic food	282:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	5	51	theme	resin	1476:1480	arg1	C					1482:1482	resin C	1476:1482	resin C (average 22.9)	1476:1497	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	51	theme	resin	1476:1480	arg1	average					1485:1491	average 22.9	1485:1496	average 22.9	1485:1496	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	0	52	theme	whey	52:55	arg1	fraction					57:64	bovine whey fraction	45:64	bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent	45:129	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	53	theme	resin	1009:1013	arg1	samples					998:1004	the three samples	988:1004	the three samples of resin accounting for average 78% of recovered sialic acid	988:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	6	54	theme	resin	1697:1701	arg1	B					1703:1703	resin B	1697:1703	resin B	1697:1703	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
32008582	1	55	theme	sialylated	198:207	arg1	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	55	theme	sialylated	198:207	arg1	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	55	theme	sialylated	198:207	arg1	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	3	56	theme	resin	604:608	arg1	C					610:610	resin C	604:610	resin C	604:610	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	1	57	theme	potential	257:265	arg1	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	57	theme	potential	257:265	arg1	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	57	theme	potential	257:265	arg1	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	5	58	theme	higher	1466:1471	arg1	times					1460:1464	2.5 times	1456:1464	2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7	1456:1585	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	59	theme	GMP	1238:1240	arg1	fraction					1242:1249	GMP fraction	1238:1249	GMP fraction from resin C	1238:1262	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	60	theme	resin	1507:1511	arg1	A					1513:1513	resin A	1507:1513	resin A	1507:1513	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	61	theme	sialic	1055:1060	arg1	acid					1062:1065	recovered sialic acid	1045:1065	recovered sialic acid	1045:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	4	62	theme	amino	761:765	arg1	composition					772:782	amino acid composition	761:782	amino acid composition which reflects the purity of the peptide	761:823	The GMP fraction obtained from each column was analyzed for amino acid composition which reflects the purity of the peptide.					
32008582	5	63	dep	fraction	1080:1087	arg1	2					1069:1069	2	1069:1069	2	1069:1069	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	64	dep	proportion	896:905	arg1	1					889:889	1	889:889	1	889:889	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	65	theme	chitin	634:639	arg1	A					641:641	chitin A	634:641	chitin A	634:641	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	7	66	theme	chitin	1795:1800	arg1	weight					1785:1790	8.6 mg/100 mg dry weight	1767:1790	8.6 mg/100 mg dry weight of chitin	1767:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	3	67	theme	chitin	657:662	arg1	C					664:664	chitin C	657:664	chitin C	657:664	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	5	68	contain	contained	1113:1121	arg1	fraction					1080:1087	the GMP fraction	1072:1087	(2) the GMP fraction from resin A or resin B	1068:1111	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	68	contain	contained	1113:1121	arg2	level					1136:1140	undetectable level	1123:1140	undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine	1123:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	1	69	theme	cheese	181:186	arg1	whey					188:191	cheese whey	181:191	cheese whey	181:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	5	70	from	A	1100:1100	arg1	fraction					1080:1087	the GMP fraction	1072:1087	(2) the GMP fraction from resin A or resin B	1068:1111	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	6	71	from	similar	1657:1663	arg1	general					1649:1655	general	1649:1655	general	1649:1655	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
32008582	3	72	theme	available	551:559	arg1	resin					576:580	anion exchange resin	561:580	commercially available anion exchange resin (resin A, resin B and resin C)	538:611	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	7	73	theme	mg	1778:1779	arg1	weight					1785:1790	8.6 mg/100 mg dry weight	1767:1790	8.6 mg/100 mg dry weight of chitin	1767:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	5	74	theme	GMP	1378:1380	arg1	capacity					1390:1397	the GMP binding capacity	1374:1397	(4) the GMP binding capacity expressed as mg/100 mg dry weight of resin	1370:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	75	theme	total	942:946	arg1	acid					965:968	total recovered sialic acid	942:968	total recovered sialic acid	942:968	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	6	76	theme	chitin	1632:1637	arg1	C					1639:1639	chitin C	1632:1639	chitin C	1632:1639	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
32008582	5	77	theme	resin	1094:1098	arg1	A					1100:1100	resin A	1094:1100	resin A	1094:1100	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	8	78	dep	efficient	1897:1905	arg1	inexpensive					1907:1917	inexpensive	1907:1917	inexpensive	1907:1917	Further research is needed to develop an efficient inexpensive method to purify GMP.					
32008582	5	79	theme	GMP	910:912	arg1	tyrosine					1356:1363	tyrosine	1356:1363	tyrosine	1356:1363	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	79	theme	GMP	910:912	arg1	arginine					1328:1335	arginine	1328:1335	arginine	1328:1335	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	79	theme	GMP	910:912	arg1	phenylalanine					1338:1350	phenylalanine	1338:1350	phenylalanine	1338:1350	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	79	theme	GMP	910:912	arg1	capacity					1390:1397	the GMP binding capacity	1374:1397	(4) the GMP binding capacity expressed as mg/100 mg dry weight of resin	1370:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	79	theme	GMP	910:912	arg1	proportion					896:905	proportion	896:905	proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids	896:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	0	80	theme	food	72:75	arg1	adsorbent					121:129	adsorbent	121:129	adsorbent	121:129	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	80	theme	food	72:75	arg1	resin					98:102	food grade anion exchange resin	72:102	food grade anion exchange resin	72:102	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	80	theme	food	72:75	arg1	chitin					108:113	chitin	108:113	chitin	108:113	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	3	81	theme	exchange	567:574	arg1	resin					576:580	anion exchange resin	561:580	commercially available anion exchange resin (resin A, resin B and resin C)	538:611	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	1	82	theme	Bovine	132:137	arg1	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	82	theme	Bovine	132:137	arg1	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	82	theme	Bovine	132:137	arg1	GMP					167:169	GMP	167:169	GMP	167:169	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	82	theme	Bovine	132:137	arg1	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	7	83	theme	GMP	1736:1738	arg1	capacity					1748:1755	a remarkable GMP binding capacity	1723:1755	a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin	1723:1800	Since chitin has a remarkable GMP binding capacity averaging 8.6 mg/100 mg dry weight of chitin, it may be a useful adsorbent for whey fractionation.					
32008582	0	84	theme	anion	83:87	arg1	adsorbent					121:129	adsorbent	121:129	adsorbent	121:129	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	84	theme	anion	83:87	arg1	resin					98:102	food grade anion exchange resin	72:102	food grade anion exchange resin	72:102	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	84	theme	anion	83:87	arg1	chitin					108:113	chitin	108:113	chitin	108:113	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	1	85	theme	dietetic	309:316	arg1	food					318:321	dietetic food	309:321	functional food as well as dietetic food	282:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	6	86	theme	chitin	1609:1614	arg1	A					1616:1616	chitin A	1609:1616	chitin A	1609:1616	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
32008582	5	87	dep	capacity	1390:1397	arg1	4					1371:1371	4	1371:1371	4	1371:1371	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	1	88	gly	sialylated	198:207	arg1	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	88	gly	sialylated	198:207	arg1	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	88	gly	sialylated	198:207	arg1	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	5	89	theme	commercial	845:854	arg1	resin					871:875	commercial anion exchange resin	845:875	commercial anion exchange resin	845:875	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	90	theme	anion	478:482	arg1	resin					493:497	food grade anion exchange resin	467:497	food grade anion exchange resin	467:497	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	0	91	from	fraction	57:64	arg1	Isolation					0:8	Isolation	0:8	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.	0:130	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	92	theme	mol	1292:1294	arg1	acids					1321:1325	contaminating amino acids	1301:1325	contaminating amino acids	1301:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	92	theme	mol	1292:1294	arg1	%					1295:1295	<1 mol%	1289:1295	<1 mol%	1289:1295	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	92	theme	mol	1292:1294	arg1	amounts					1280:1286	small amounts	1274:1286	small amounts (<1 mol%) of contaminating amino acids	1274:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	93	theme	sialic	958:963	arg1	acid					965:968	total recovered sialic acid	942:968	total recovered sialic acid	942:968	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	94	theme	exchange	862:869	arg1	resin					871:875	commercial anion exchange resin	845:875	commercial anion exchange resin	845:875	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	95	theme	food	467:470	arg1	resin					493:497	food grade anion exchange resin	467:497	food grade anion exchange resin	467:497	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	96	theme	amino	1159:1163	arg1	arginine					1207:1214	arginine	1207:1214	arginine	1207:1214	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	96	theme	amino	1159:1163	arg1	histidine					1196:1204	histidine	1196:1204	histidine	1196:1204	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	96	theme	amino	1159:1163	arg1	tyrosine					1220:1227	tyrosine	1220:1227	tyrosine	1220:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	96	theme	amino	1159:1163	arg1	phenylalanine					1181:1193	phenylalanine	1181:1193	phenylalanine	1181:1193	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	96	theme	amino	1159:1163	arg1	acids					1165:1169	contaminating amino acids	1145:1169	contaminating amino acids including phenylalanine, histidine, arginine and tyrosine	1145:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	97	theme	soluble	408:414	arg1	SWF					431:433	SWF	431:433	SWF	431:433	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	2	97	theme	soluble	408:414	arg1	fraction					421:428	soluble whey fraction	408:428	soluble whey fraction (SWF)	408:434	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	98	theme	small	1274:1278	arg1	acids					1321:1325	contaminating amino acids	1301:1325	contaminating amino acids	1301:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	98	theme	small	1274:1278	arg1	%					1295:1295	<1 mol%	1289:1295	<1 mol%	1289:1295	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	98	theme	small	1274:1278	arg1	amounts					1280:1286	small amounts	1274:1286	small amounts (<1 mol%) of contaminating amino acids	1274:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	0	99	theme	glycomacropeptide	22:38	arg1	Isolation					0:8	Isolation	0:8	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.	0:130	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	100	theme	average	1030:1036	arg1	acid					1062:1065	recovered sialic acid	1045:1065	recovered sialic acid	1045:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	100	theme	average	1030:1036	arg1	%					1040:1040	average 78%	1030:1040	average 78% of recovered sialic acid	1030:1065	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	101	dep	similar	974:980	arg1	3					1231:1231	3	1231:1231	3	1231:1231	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	102	theme	acids	1321:1325	arg1	acids					1321:1325	contaminating amino acids	1301:1325	contaminating amino acids	1301:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	102	theme	acids	1321:1325	arg1	%					1295:1295	<1 mol%	1289:1295	<1 mol%	1289:1295	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	102	theme	acids	1321:1325	arg1	amounts					1280:1286	small amounts	1274:1286	small amounts (<1 mol%) of contaminating amino acids	1274:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	103	theme	resin	592:596	arg1	B					598:598	resin B	592:598	resin B	592:598	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	5	104	theme	undetectable	1123:1134	arg1	level					1136:1140	undetectable level	1123:1140	undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine	1123:1227	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	105	theme	dry	1422:1424	arg1	weight					1426:1431	mg/100 mg dry weight	1412:1431	mg/100 mg dry weight of resin	1412:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	0	106	theme	bovine	45:50	arg1	fraction					57:64	bovine whey fraction	45:64	bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent	45:129	Isolation of κ-casein glycomacropeptide from bovine whey fraction using food grade anion exchange resin and chitin as an adsorbent.					
32008582	8	107	theme	efficient	1897:1905	arg1	method					1919:1924	an efficient inexpensive method	1894:1924	an efficient inexpensive method to purify GMP	1894:1938	Further research is needed to develop an efficient inexpensive method to purify GMP.					
32008582	5	108	theme	resin	1436:1440	arg1	weight					1426:1431	mg/100 mg dry weight	1412:1431	mg/100 mg dry weight of resin	1412:1440	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	2	109	dep	purity	376:381	arg1	GMP					383:385	GMP	383:385	high purity GMP	371:385	This study was undertaken to determine whether high purity GMP can be isolated from soluble whey fraction (SWF) using column chromatography on food grade anion exchange resin and chitin as an adsorbent.					
32008582	5	110	with	A	1513:1513	arg1	difference					1534:1543	no difference	1531:1543	no difference between resin A and resin B averaging 8.7	1531:1585	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	5	111	theme	resin	1565:1569	arg1	B					1571:1571	resin B	1565:1571	resin B	1565:1571	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	3	112	theme	anion	561:565	arg1	resin					576:580	anion exchange resin	561:580	commercially available anion exchange resin (resin A, resin B and resin C)	538:611	Samples of commercially available anion exchange resin (resin A, resin B and resin C) and those of chitin (chitin A, chitin B and chitin C) were examined in this experiment.					
32008582	5	113	theme	contaminating	1301:1313	arg1	acids					1321:1325	contaminating amino acids	1301:1325	contaminating amino acids	1301:1325	Major findings for commercial anion exchange resin were that: (1) the proportion of GMP monitored as sialic acid in total recovered sialic acid was similar among the three samples of resin accounting for average 78% of recovered sialic acid; (2) the GMP fraction from resin A or resin B contained undetectable level of contaminating amino acids including phenylalanine, histidine, arginine and tyrosine; (3) the GMP fraction from resin C contained small amounts (<1 mol%) of contaminating amino acids, arginine, phenylalanine and tyrosine; and (4) the GMP binding capacity expressed as mg/100 mg dry weight of resin was more than 2.5 times higher in resin C (average 22.9) than in resin A or resin B with no difference between resin A and resin B averaging 8.7.					
32008582	1	114	theme	phosphorylated	209:222	arg1	peptide					224:230	a sialylated phosphorylated peptide	196:230	a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food	196:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	114	theme	phosphorylated	209:222	arg1	ingredient					267:276	a potential ingredient	255:276	a potential ingredient for functional food as well as dietetic food	255:321	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	1	114	theme	phosphorylated	209:222	arg1	glycomacropeptide					148:164	Bovine κ-casein glycomacropeptide	132:164	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey	132:191	Bovine κ-casein glycomacropeptide (GMP) found in cheese whey is a sialylated phosphorylated peptide which is thought to be a potential ingredient for functional food as well as dietetic food.					
32008582	6	115	theme	resin	1685:1689	arg1	A					1691:1691	resin A	1685:1691	resin A	1685:1691	Results obtained for chitin A, chitin B and chitin C were in general similar to those found with resin A and resin B.					
33673999	0	0	theme	interface	96:104	arg1	reaction					106:113	interface reaction	96:113	interface reaction of oxidized starch	96:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	1	1	theme	carboxyl	348:355	arg1	content					357:363	different carboxyl content	338:363	different carboxyl content	338:363	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	4	2	theme	XNBR	870:873	arg1	composites					875:884	the sulfur-free XNBR composites	854:884	the sulfur-free XNBR composites achieved 3.04 and 1.99 times	854:913	Noteworthy, the sulfur-free XNBR composites achieved 3.04 and 1.99 times increase for tensile strength and elongation at break compared with neat XNBR.					
33673999	1	3	theme	simultaneous	175:186	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	3	4	theme	antibacterial	818:830	arg1	activity					832:839	a significant antibacterial activity	804:839	a significant antibacterial activity	804:839	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	3	5	theme	OSTs	694:697	arg1	OSTs					694:697	OSTs	694:697	OSTs which provided a low pH surroundings to inhibit the growth of bacteria	694:768	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	3	5	theme	OSTs	694:697	arg1	groups					684:689	the carboxyl groups	671:689	the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria	671:768	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	3	6	theme	carboxyl	675:682	arg1	OSTs					694:697	OSTs	694:697	OSTs which provided a low pH surroundings to inhibit the growth of bacteria	694:768	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	3	6	theme	carboxyl	675:682	arg1	groups					684:689	the carboxyl groups	671:689	the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria	671:768	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	4	7	theme	sulfur-free	858:868	arg1	composites					875:884	the sulfur-free XNBR composites	854:884	the sulfur-free XNBR composites achieved 3.04 and 1.99 times	854:913	Noteworthy, the sulfur-free XNBR composites achieved 3.04 and 1.99 times increase for tensile strength and elongation at break compared with neat XNBR.					
33673999	1	8	theme	strengthened	188:199	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	2	9	theme	networks	521:528	arg1	formation					491:499	The formation	487:499	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites	487:591	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites were confirmed by FT-IR, XRD, XPS, SEM-EDS and TEM.					
33673999	2	10	theme	Zinc-carboxylate	535:550	arg1	polymers					552:559	"Zinc-carboxylate polymers	534:559	"Zinc-carboxylate polymers	534:559	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites were confirmed by FT-IR, XRD, XPS, SEM-EDS and TEM.					
33673999	1	11	with	starch	320:325	arg1	content					357:363	different carboxyl content	338:363	different carboxyl content	338:363	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	1	12	used	utilized	386:393	arg2	ZnO					377:379	ZnO	377:379	ZnO	377:379	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	1	12	used	utilized	386:393	arg2	meanwhile					366:374	meanwhile	366:374	meanwhile	366:374	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	0	13	theme	starch	127:132	arg1	reaction					106:113	interface reaction	96:113	interface reaction of oxidized starch	96:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	3	14	theme	low	716:718	arg1	pH					720:721	a low pH surroundings to inhibit the growth of bacteria	714:768	a low pH surroundings to inhibit the growth of bacteria	714:768	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	1	15	theme	starch	470:475	arg1	compatibility					453:465	compatibility	453:465	compatibility of starch and XNBR	453:484	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	6	16	theme	protective	1182:1191	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	16	theme	protective	1182:1191	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	17	used	utilized	1158:1165	arg2	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	17	used	utilized	1158:1165	arg2	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	18	theme	new	1091:1093	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	18	theme	new	1091:1093	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	0	19	theme	oxidized	118:125	arg1	starch					127:132	oxidized starch	118:132	oxidized starch	118:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	4	20	from	break	963:967	arg1	elongation					949:958	elongation	949:958	elongation	949:958	Noteworthy, the sulfur-free XNBR composites achieved 3.04 and 1.99 times increase for tensile strength and elongation at break compared with neat XNBR.					
33673999	4	20	from	break	963:967	arg1	strength					936:943	tensile strength	928:943	tensile strength	928:943	Noteworthy, the sulfur-free XNBR composites achieved 3.04 and 1.99 times increase for tensile strength and elongation at break compared with neat XNBR.					
33673999	6	21	theme	medical	1174:1180	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	21	theme	medical	1174:1180	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	1	22	theme	toughened	205:213	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	0	23	theme	antibacterial	9:21	arg1	performances					38:49	antibacterial and mechanical performances	9:49	antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch	9:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	4	24	theme	neat	983:986	arg1	XNBR					988:991	neat XNBR	983:991	neat XNBR	983:991	Noteworthy, the sulfur-free XNBR composites achieved 3.04 and 1.99 times increase for tensile strength and elongation at break compared with neat XNBR.					
33673999	1	25	theme	XNBR	481:484	arg1	compatibility					453:465	compatibility	453:465	compatibility of starch and XNBR	453:484	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	1	26	theme	carboxylated	215:226	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	1	27	theme	nitrile	228:234	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	0	28	theme	mechanical	27:36	arg1	performances					38:49	antibacterial and mechanical performances	9:49	antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch	9:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	3	29	theme	significant	806:816	arg1	activity					832:839	a significant antibacterial activity	804:839	a significant antibacterial activity	804:839	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	1	30	theme	butadiene	236:244	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	6	31	theme	facile	1118:1123	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	31	theme	facile	1118:1123	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	1	32	theme	oxidized	311:318	arg1	OST					328:330	OST	328:330	OST	328:330	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	1	32	theme	oxidized	311:318	arg1	starch					320:325	oxidized starch	311:325	oxidized starch (OST) with different carboxyl content	311:363	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	4	33	theme	tensile	928:934	arg1	strength					936:943	tensile strength	928:943	tensile strength	928:943	Noteworthy, the sulfur-free XNBR composites achieved 3.04 and 1.99 times increase for tensile strength and elongation at break compared with neat XNBR.					
33673999	6	34	theme	sustainable	1095:1105	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	34	theme	sustainable	1095:1105	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	1	35	theme	rubber	246:251	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	5	36	theme	simultaneous	1011:1022	arg1	strengthened					1024:1035	simultaneous strengthened	1011:1035	simultaneous strengthened	1011:1035	The mechanism of simultaneous strengthened and toughened for composites had been proposed.					
33673999	2	37	theme	polymers	552:559	arg1	formation					491:499	The formation	487:499	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites	487:591	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites were confirmed by FT-IR, XRD, XPS, SEM-EDS and TEM.					
33673999	5	38	theme	strengthened	1024:1035	arg1	mechanism					998:1006	The mechanism	994:1006	The mechanism of simultaneous strengthened and toughened for composites	994:1064	The mechanism of simultaneous strengthened and toughened for composites had been proposed.					
33673999	1	39	theme	XNBR	254:257	arg1	composites					260:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites	175:269	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	0	40	theme	nitrile	67:73	arg1	rubber					85:90	carboxylated nitrile butadiene rubber	54:90	carboxylated nitrile butadiene rubber via interface reaction of oxidized starch	54:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	2	41	theme	cross-link	510:519	arg1	networks					521:528	ionic cross-link networks	504:528	ionic cross-link networks	504:528	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites were confirmed by FT-IR, XRD, XPS, SEM-EDS and TEM.					
33673999	2	42	theme	ionic	504:508	arg1	networks					521:528	ionic cross-link networks	504:528	ionic cross-link networks	504:528	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites were confirmed by FT-IR, XRD, XPS, SEM-EDS and TEM.					
33673999	0	43	theme	carboxylated	54:65	arg1	rubber					85:90	carboxylated nitrile butadiene rubber	54:90	carboxylated nitrile butadiene rubber via interface reaction of oxidized starch	54:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	1	44	theme	in-situ	410:416	arg1	reaction					430:437	the in-situ interfacial reaction	406:437	the in-situ interfacial reaction for improving compatibility of starch and XNBR	406:484	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	5	45	theme	toughened	1041:1049	arg1	mechanism					998:1006	The mechanism	994:1006	The mechanism of simultaneous strengthened and toughened for composites	994:1064	The mechanism of simultaneous strengthened and toughened for composites had been proposed.					
33673999	1	46	theme	interfacial	418:428	arg1	reaction					430:437	the in-situ interfacial reaction	406:437	the in-situ interfacial reaction for improving compatibility of starch and XNBR	406:484	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	6	47	theme	fabricated	1125:1134	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	47	theme	fabricated	1125:1134	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	2	48	theme	XNBR/OST/ZnO	569:580	arg1	composites					582:591	the XNBR/OST/ZnO composites	565:591	the XNBR/OST/ZnO composites	565:591	The formation of ionic cross-link networks and "Zinc-carboxylate polymers" in the XNBR/OST/ZnO composites were confirmed by FT-IR, XRD, XPS, SEM-EDS and TEM.					
33673999	1	49	theme	antibacterial	148:160	arg1	activity					162:169	antibacterial activity	148:169	antibacterial activity	148:169	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	0	50	theme	rubber	85:90	arg1	performances					38:49	antibacterial and mechanical performances	9:49	antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch	9:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	0	51	theme	butadiene	75:83	arg1	rubber					85:90	carboxylated nitrile butadiene rubber	54:90	carboxylated nitrile butadiene rubber via interface reaction of oxidized starch	54:132	Improved antibacterial and mechanical performances of carboxylated nitrile butadiene rubber via interface reaction of oxidized starch.					
33673999	1	52	theme	different	338:346	arg1	content					357:363	different carboxyl content	338:363	different carboxyl content	338:363	To fabricate antibacterial activity and simultaneous strengthened and toughened carboxylated nitrile butadiene rubber (XNBR) composites, starch was oxidized by H2O2 to achieve oxidized starch (OST) with different carboxyl content, meanwhile, ZnO were utilized to promote the in-situ interfacial reaction for improving compatibility of starch and XNBR.					
33673999	3	53	theme	XNBR/OST/ZnO	771:782	arg1	composites					784:793	XNBR/OST/ZnO composites	771:793	XNBR/OST/ZnO composites	771:793	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33673999	6	54	theme	green	1108:1112	arg1	appliance					1193:1201	the medical protective appliance	1170:1201	the medical protective appliance to against the bacteria	1170:1225	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	6	54	theme	green	1108:1112	arg1	XNBR/OST/ZnO					1136:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO	1085:1147	These new sustainable, green and facile fabricated XNBR/OST/ZnO could be utilized as the medical protective appliance to against the bacteria.					
33673999	3	55	theme	bacteria	761:768	arg1	growth					751:756	the growth	747:756	the growth of bacteria	747:768	Interestingly, because of the carboxyl groups of OSTs which provided a low pH surroundings to inhibit the growth of bacteria, XNBR/OST/ZnO composites achieved a significant antibacterial activity.					
33310029	9	0	theme	microbiota	1358:1367	arg1	significance					1338:1349	the significance	1334:1349	the significance of gut microbiota in the mediation of constipation	1334:1400	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	10	1	theme	gut	1516:1518	arg1	microbiota					1520:1529	gut microbiota	1516:1529	gut microbiota	1516:1529	In summary, this study leads us to propose that lactulose may improve constipation through a prebiotic effect on gut microbiota and intestinal metabolites.					
33310029	6	2	theme	chromatography	645:658	arg1	analysis					714:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis	641:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis	641:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	0	3	theme	loperamide-induced	78:95	arg1	constipation					97:108	loperamide-induced constipation	78:108	loperamide-induced constipation in mice	78:116	Modulation of gut microbiota and intestinal metabolites by lactulose improves loperamide-induced constipation in mice.					
33310029	6	4	theme	Gas	641:643	arg1	chromatography					645:658	Gas chromatography	641:658	Gas chromatography	641:658	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	6	4	theme	Gas	641:643	arg1	GC/LC-MS					704:711	GC/LC-MS	704:711	GC/LC-MS	704:711	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	9	5	theme	-treated	1310:1317	arg1	mice					1319:1322	loperamide- or (loperamide + lactulose)-treated mice	1271:1322	loperamide- or (loperamide + lactulose)-treated mice	1271:1322	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	9	6	theme	loperamide-	1271:1281	arg1	mice					1319:1322	loperamide- or (loperamide + lactulose)-treated mice	1271:1322	loperamide- or (loperamide + lactulose)-treated mice	1271:1322	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	8	7	theme	therapeutic	996:1006	arg1	effects					1008:1014	These therapeutic effects	990:1014	These therapeutic effects	990:1014	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	5	8	theme	rDNA	619:622	arg1	sequencing					629:638	rDNA gene sequencing	619:638	rDNA gene sequencing	619:638	The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing.					
33310029	6	9	used	used	727:730	arg2	analysis					714:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis	641:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis	641:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	10	10	theme	prebiotic	1496:1504	arg1	effect					1506:1511	a prebiotic effect	1494:1511	a prebiotic effect on gut microbiota and intestinal metabolites	1494:1556	In summary, this study leads us to propose that lactulose may improve constipation through a prebiotic effect on gut microbiota and intestinal metabolites.					
33310029	5	11	theme	gene	624:627	arg1	sequencing					629:638	rDNA gene sequencing	619:638	rDNA gene sequencing	619:638	The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing.					
33310029	7	12	from	metabolisms	935:945	arg1	tract					983:987	the intestinal tract	968:987	the intestinal tract	968:987	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	7	13	theme	water	950:954	arg1	metabolisms					935:945	metabolisms	935:945	metabolisms of water and salt in the intestinal tract	935:987	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	5	14	from	effect	542:547	arg1	composition					569:579	the composition	565:579	the composition of gut microbiota	565:597	The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing.					
33310029	3	15	theme	BALB/c	364:369	arg1	mice					371:374	BALB/c mice	364:374	BALB/c mice with constipation induced by loperamide	364:414	BALB/c mice with constipation induced by loperamide were orally treated with lactulose for four weeks.					
33310029	9	16	theme	constipation	1389:1400	arg1	mediation					1376:1384	the mediation	1372:1384	the mediation of constipation	1372:1400	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	10	17	theme	intestinal	1535:1544	arg1	metabolites					1546:1556	intestinal metabolites	1535:1556	intestinal metabolites	1535:1556	In summary, this study leads us to propose that lactulose may improve constipation through a prebiotic effect on gut microbiota and intestinal metabolites.					
33310029	5	18	theme	microbiota	588:597	arg1	composition					569:579	the composition	565:579	the composition of gut microbiota	565:597	The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing.					
33310029	2	19	theme	lactulose	270:278	arg1	effect					260:265	the improving effect	246:265	the improving effect of lactulose on constipation	246:294	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	7	20	theme	gut	909:911	arg1	barrier					913:919	gut barrier	909:919	gut barrier	909:919	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	4	21	theme	constipation-related	492:511	arg1	factors					513:519	the constipation-related factors	488:519	the constipation-related factors	488:519	After the treatment, the constipation-related factors were determined.					
33310029	8	22	theme	intestinal	1125:1134	arg1	metabolites					1136:1146	intestinal metabolites	1125:1146	intestinal metabolites	1125:1146	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	8	23	theme	microbiota	1052:1061	arg1	dysfunction					1063:1073	the reversed gut microbiota dysfunction	1035:1073	the reversed gut microbiota dysfunction	1035:1073	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	2	24	theme	gut	321:323	arg1	microbiota					325:334	gut microbiota	321:334	gut microbiota	321:334	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	1	25	theme	clinical	181:188	arg1	treatment					190:198	clinical treatment	181:198	clinical treatment for constipation	181:215	Lactulose is a common laxative and has been widely applied to clinical treatment for constipation.					
33310029	10	26	from	effect	1506:1511	arg1	microbiota					1520:1529	gut microbiota	1516:1529	gut microbiota	1516:1529	In summary, this study leads us to propose that lactulose may improve constipation through a prebiotic effect on gut microbiota and intestinal metabolites.					
33310029	10	26	from	effect	1506:1511	arg1	metabolites					1546:1556	intestinal metabolites	1535:1556	intestinal metabolites	1535:1556	In summary, this study leads us to propose that lactulose may improve constipation through a prebiotic effect on gut microbiota and intestinal metabolites.					
33310029	7	27	theme	intestinal	972:981	arg1	tract					983:987	the intestinal tract	968:987	the intestinal tract	968:987	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	0	28	theme	microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of gut microbiota and intestinal metabolites by lactulose	0:67	Modulation of gut microbiota and intestinal metabolites by lactulose improves loperamide-induced constipation in mice.					
33310029	8	29	theme	reversed	1039:1046	arg1	dysfunction					1063:1073	the reversed gut microbiota dysfunction	1035:1073	the reversed gut microbiota dysfunction	1035:1073	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	0	30	theme	gut	14:16	arg1	microbiota					18:27	gut microbiota	14:27	gut microbiota	14:27	Modulation of gut microbiota and intestinal metabolites by lactulose improves loperamide-induced constipation in mice.					
33310029	7	31	theme	intestinal	843:852	arg1	motility					854:861	intestinal motility	843:861	intestinal motility	843:861	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	8	32	theme	metabolites	1136:1146	arg1	production					1111:1120	the production	1107:1120	the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites	1107:1220	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	0	33	from	constipation	97:108	arg1	mice					113:116	mice	113:116	mice	113:116	Modulation of gut microbiota and intestinal metabolites by lactulose improves loperamide-induced constipation in mice.					
33310029	9	34	theme	intestinal	1249:1258	arg1	flora					1260:1264	intestinal flora	1249:1264	intestinal flora	1249:1264	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	7	35	theme	inflammatory	875:886	arg1	responses					888:896	inflammatory responses	875:896	inflammatory responses	875:896	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	0	36	theme	intestinal	33:42	arg1	metabolites					44:54	intestinal metabolites	33:54	intestinal metabolites	33:54	Modulation of gut microbiota and intestinal metabolites by lactulose improves loperamide-induced constipation in mice.					
33310029	2	37	from	effect	260:265	arg1	constipation					283:294	constipation	283:294	constipation	283:294	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	8	38	theme	gut	1048:1050	arg1	dysfunction					1063:1073	the reversed gut microbiota dysfunction	1035:1073	the reversed gut microbiota dysfunction	1035:1073	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	7	39	theme	salt	960:963	arg1	metabolisms					935:945	metabolisms	935:945	metabolisms of water and salt in the intestinal tract	935:987	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	8	40	theme	tryptophan	1199:1208	arg1	catabolites					1210:1220	tryptophan catabolites	1199:1220	tryptophan catabolites	1199:1220	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	7	41	with	treatment	786:794	arg1	lactulose					821:829	lactulose	821:829	lactulose	821:829	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	8	42	theme	short-chain	1170:1180	arg1	acids					1188:1192	short-chain fatty acids	1170:1192	short-chain fatty acids	1170:1192	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	3	43	with	mice	371:374	arg1	constipation					381:392	constipation	381:392	constipation induced by loperamide	381:414	BALB/c mice with constipation induced by loperamide were orally treated with lactulose for four weeks.					
33310029	2	44	theme	metabolites	351:361	arg1	mediation					308:316	the mediation	304:316	the mediation of gut microbiota and intestinal metabolites	304:361	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	0	45	theme	metabolites	44:54	arg1	Modulation					0:9	Modulation	0:9	Modulation of gut microbiota and intestinal metabolites by lactulose	0:67	Modulation of gut microbiota and intestinal metabolites by lactulose improves loperamide-induced constipation in mice.					
33310029	7	46	theme	mice	811:814	arg1	treatment					786:794	The treatment	782:794	The treatment of constipated mice with lactulose	782:829	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	2	47	theme	intestinal	340:349	arg1	metabolites					351:361	intestinal metabolites	340:361	intestinal metabolites	340:361	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	9	48	theme	flora	1260:1264	arg1	depletion					1236:1244	the depletion	1232:1244	the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice	1232:1322	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	5	49	theme	gut	584:586	arg1	microbiota					588:597	gut microbiota	584:597	gut microbiota	584:597	The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing.					
33310029	1	50	theme	common	134:139	arg1	laxative					141:148	a common laxative	132:148	a common laxative	132:148	Lactulose is a common laxative and has been widely applied to clinical treatment for constipation.					
33310029	1	50	theme	common	134:139	arg1	Lactulose					119:127	Lactulose	119:127	Lactulose	119:127	Lactulose is a common laxative and has been widely applied to clinical treatment for constipation.					
33310029	6	51	theme	spectrometer	690:701	arg1	analysis					714:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis	641:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis	641:721	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	8	52	theme	fatty	1182:1186	arg1	acids					1188:1192	short-chain fatty acids	1170:1192	short-chain fatty acids	1170:1192	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	6	53	theme	intestinal	758:767	arg1	metabolites					769:779	intestinal metabolites	758:779	intestinal metabolites	758:779	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	2	54	theme	microbiota	325:334	arg1	mediation					308:316	the mediation	304:316	the mediation of gut microbiota and intestinal metabolites	304:361	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	6	55	theme	chromatography-mass	670:688	arg1	spectrometer					690:701	liquid chromatography-mass spectrometer	663:701	liquid chromatography-mass spectrometer	663:701	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	7	56	theme	constipated	799:809	arg1	mice					811:814	constipated mice	799:814	constipated mice	799:814	The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract.					
33310029	9	57	from	significance	1338:1349	arg1	mediation					1376:1384	the mediation	1372:1384	the mediation of constipation	1372:1400	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	2	58	theme	improving	250:258	arg1	effect					260:265	the improving effect	246:265	the improving effect of lactulose on constipation	246:294	This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites.					
33310029	6	59	theme	metabolites	769:779	arg1	quantification					740:753	the quantification	736:753	the quantification of intestinal metabolites	736:779	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	6	60	theme	liquid	663:668	arg1	spectrometer					690:701	liquid chromatography-mass spectrometer	663:701	liquid chromatography-mass spectrometer	663:701	Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites.					
33310029	9	61	theme	gut	1354:1356	arg1	microbiota					1358:1367	gut microbiota	1354:1367	gut microbiota	1354:1367	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33310029	5	62	theme	lactulose	552:560	arg1	effect					542:547	The effect	538:547	The effect of lactulose on the composition of gut microbiota	538:597	The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing.					
33310029	8	63	theme	bile	1158:1161	arg1	acids					1163:1167	bile acids	1158:1167	bile acids	1158:1167	These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites.					
33310029	9	64	from	mice	1319:1322	arg1	depletion					1236:1244	the depletion	1232:1244	the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice	1232:1322	Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation.					
33755808	1	0	theme	milk	131:134	arg1	yield					136:140	milk yield	131:140	milk yield	131:140	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	3	1	theme	±	566:566	arg1	%					572:572	4.89 ± 0.24%	561:572	4.89 ± 0.24%	561:572	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	1	theme	±	566:566	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	2	theme	±	617:617	arg1	units					624:628	4.12 ± 2.06 units	612:628	4.12 ± 2.06 units	612:628	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	2	theme	±	617:617	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	0	3	theme	cows	67:70	arg1	yield					34:38	daily milk yield	23:38	daily milk yield	23:38	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	0	3	theme	cows	67:70	arg1	components					44:53	components	44:53	components of Holstein cows in Morocco	44:81	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	0	4	from	effects	12:18	arg1	yield					34:38	daily milk yield	23:38	daily milk yield	23:38	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	0	4	from	effects	12:18	arg1	components					44:53	components	44:53	components of Holstein cows in Morocco	44:81	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	5	5	theme	concentration	838:850	arg1	exception					821:829	the exception	817:829	the exception of MUN concentration	817:850	Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration.					
33755808	11	6	theme	Protein	1465:1471	arg1	contents					1481:1488	Protein and SNF contents	1465:1488	contents	1481:1488	Protein and SNF contents and SCS attained their lowest values between days 35 and 65, while fat content between days 65 and 95.					
33755808	6	7	theme	Milk	853:856	arg1	yield					858:862	Milk yield	853:862	Milk yield	853:862	Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities.					
33755808	3	8	theme	±	552:552	arg1	%					558:558	3.02 ± 0.34%	547:558	3.02 ± 0.34%	547:558	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	8	theme	±	552:552	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	4	9	theme	herd	660:663	arg1	effects					649:655	The effects	645:655	The effects of herd and calving year	645:680	The effects of herd and calving year were found to be significant on all the studied traits.					
33755808	4	9	theme	herd	660:663	arg1	significant					699:709	significant	699:709	significant	699:709	The effects of herd and calving year were found to be significant on all the studied traits.					
33755808	9	10	theme	lactation	1314:1322	arg1	stage					1305:1309	stage	1305:1309	stage of lactation	1305:1322	All the studied traits changed significantly according to stage of lactation.					
33755808	2	11	theme	urea	448:451	arg1	MUN					463:465	MUN	463:465	MUN	463:465	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	11	theme	urea	448:451	arg1	nitrogen					453:460	milk urea nitrogen	443:460	milk urea nitrogen (MUN)	443:466	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	0	12	from	components	44:53	arg1	Morocco					75:81	Morocco	75:81	Morocco	75:81	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	2	13	theme	Holstein	326:333	arg1	cows					335:338	6343 Holstein cows	321:338	6343 Holstein cows	321:338	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	3	14	theme	4.12	612:615	arg1	units					624:628	4.12 ± 2.06 units	612:628	4.12 ± 2.06 units	612:628	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	14	theme	4.12	612:615	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	2	15	theme	milk	443:446	arg1	MUN					463:465	MUN	463:465	MUN	463:465	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	15	theme	milk	443:446	arg1	nitrogen					453:460	milk urea nitrogen	443:460	milk urea nitrogen (MUN)	443:466	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	16	from	records	310:316	arg1	herds					347:351	162 herds	343:351	162 herds	343:351	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	8	17	theme	SNF	1191:1193	arg1	content					1195:1201	SNF content	1191:1201	SNF content	1191:1201	Milk yield and MUN were highest for cows calving during humid season, whereas protein content, SNF content, and SCS tended to be higher in dry season.					
33755808	10	18	theme	content	1361:1367	arg1	peaks					1329:1333	The peaks	1325:1333	The peaks of milk yield and lactose content	1325:1367	The peaks of milk yield and lactose content occurred between 35 and 65 days, whereas that of MUN between 95 and 125 days after parturition.					
33755808	0	19	from	yield	34:38	arg1	Morocco					75:81	Morocco	75:81	Morocco	75:81	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	8	20	theme	Milk	1096:1099	arg1	yield					1101:1105	Milk yield	1096:1105	Milk yield	1096:1105	Milk yield and MUN were highest for cows calving during humid season, whereas protein content, SNF content, and SCS tended to be higher in dry season.					
33755808	8	21	theme	dry	1235:1237	arg1	season					1239:1244	dry season	1235:1244	dry season	1235:1244	Milk yield and MUN were highest for cows calving during humid season, whereas protein content, SNF content, and SCS tended to be higher in dry season.					
33755808	2	22	theme	fat	395:397	arg1	contents					383:390	contents	383:390	contents of fat, protein, lactose and solids-not-fat (SNF)	383:440	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	22	theme	fat	395:397	arg1	nitrogen					453:460	milk urea nitrogen	443:460	milk urea nitrogen (MUN)	443:466	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	22	theme	fat	395:397	arg1	score					486:490	somatic cell score	473:490	somatic cell score (SCS)	473:496	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	22	theme	fat	395:397	arg1	yield					376:380	milk yield	371:380	milk yield	371:380	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	22	theme	fat	395:397	arg1	MUN					463:465	MUN	463:465	MUN	463:465	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	22	theme	fat	395:397	arg1	SCS					493:495	SCS	493:495	SCS	493:495	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	4	23	theme	studied	722:728	arg1	traits					730:735	all the studied traits	714:735	all the studied traits	714:735	The effects of herd and calving year were found to be significant on all the studied traits.					
33755808	12	24	theme	environmental	1621:1633	arg1	factors					1635:1641	these environmental factors	1615:1641	these environmental factors	1615:1641	It was concluded that these environmental factors should be taken into consideration if the milk yield and quality in Morocco is to be optimized.					
33755808	2	25	theme	solids-not-fat	421:434	arg1	contents					383:390	contents	383:390	contents of fat, protein, lactose and solids-not-fat (SNF)	383:440	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	25	theme	solids-not-fat	421:434	arg1	nitrogen					453:460	milk urea nitrogen	443:460	milk urea nitrogen (MUN)	443:466	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	25	theme	solids-not-fat	421:434	arg1	score					486:490	somatic cell score	473:490	somatic cell score (SCS)	473:496	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	25	theme	solids-not-fat	421:434	arg1	yield					376:380	milk yield	371:380	milk yield	371:380	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	25	theme	solids-not-fat	421:434	arg1	MUN					463:465	MUN	463:465	MUN	463:465	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	25	theme	solids-not-fat	421:434	arg1	SCS					493:495	SCS	493:495	SCS	493:495	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	7	26	theme	other	1023:1027	arg1	variables					1037:1045	all the other studied variables	1015:1045	all the other studied variables	1015:1045	Except fat and lactose contents, all the other studied variables were significantly influenced by calving season.					
33755808	6	27	dep	increased	903:911	arg1	whereas					926:932	whereas	926:932	whereas	926:932	Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities.					
33755808	11	28	theme	fat	1557:1559	arg1	content					1561:1567	fat content	1557:1567	fat content between days 65 and 95	1557:1590	Protein and SNF contents and SCS attained their lowest values between days 35 and 65, while fat content between days 65 and 95.					
33755808	0	29	theme	Non-genetic	0:10	arg1	effects					12:18	Non-genetic effects	0:18	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.	0:82	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	1	30	theme	environmental	239:251	arg1	factors					253:259	environmental factors	239:259	environmental factors influencing them	239:276	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	8	31	theme	humid	1152:1156	arg1	season					1158:1163	humid season	1152:1163	humid season	1152:1163	Milk yield and MUN were highest for cows calving during humid season, whereas protein content, SNF content, and SCS tended to be higher in dry season.					
33755808	2	32	theme	162	343:345	arg1	herds					347:351	162 herds	343:351	162 herds	343:351	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	0	33	theme	daily	23:27	arg1	yield					34:38	daily milk yield	23:38	daily milk yield	23:38	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	1	34	theme	factors	253:259	arg1	effects					228:234	the effects	224:234	the effects of environmental factors influencing them	224:276	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	12	35	theme	milk	1685:1688	arg1	yield					1690:1694	milk yield	1685:1694	milk yield	1685:1694	It was concluded that these environmental factors should be taken into consideration if the milk yield and quality in Morocco is to be optimized.					
33755808	6	36	theme	fat	865:867	arg1	content					869:875	fat content	865:875	fat content	865:875	Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities.					
33755808	12	37	dep	yield	1690:1694	arg1	the					1681:1683	the	1681:1683	the	1681:1683	It was concluded that these environmental factors should be taken into consideration if the milk yield and quality in Morocco is to be optimized.					
33755808	9	38	theme	studied	1255:1261	arg1	traits					1263:1268	All the studied traits	1247:1268	All the studied traits	1247:1268	All the studied traits changed significantly according to stage of lactation.					
33755808	7	39	theme	fat	989:991	arg1	contents					1005:1012	fat and lactose contents	989:1012	fat and lactose contents	989:1012	Except fat and lactose contents, all the other studied variables were significantly influenced by calving season.					
33755808	1	40	theme	Holstein	170:177	arg1	cows					179:182	Holstein cows	170:182	Holstein cows	170:182	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	10	41	theme	milk	1338:1341	arg1	yield					1343:1347	milk yield	1338:1347	milk yield	1338:1347	The peaks of milk yield and lactose content occurred between 35 and 65 days, whereas that of MUN between 95 and 125 days after parturition.					
33755808	11	42	theme	SNF	1477:1479	arg1	contents					1481:1488	Protein and SNF contents	1465:1488	contents	1481:1488	Protein and SNF contents and SCS attained their lowest values between days 35 and 65, while fat content between days 65 and 95.					
33755808	1	43	theme	cows	179:182	arg1	levels					160:165	constituents' levels	146:165	constituents' levels	146:165	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	1	43	theme	cows	179:182	arg1	yield					136:140	milk yield	131:140	milk yield	131:140	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	10	44	theme	yield	1343:1347	arg1	peaks					1329:1333	The peaks	1325:1333	The peaks of milk yield and lactose content	1325:1367	The peaks of milk yield and lactose content occurred between 35 and 65 days, whereas that of MUN between 95 and 125 days after parturition.					
33755808	2	45	theme	milk	371:374	arg1	yield					376:380	milk yield	371:380	milk yield	371:380	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	0	46	theme	milk	29:32	arg1	yield					34:38	daily milk yield	23:38	daily milk yield	23:38	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	2	47	theme	lactose	409:415	arg1	contents					383:390	contents	383:390	contents of fat, protein, lactose and solids-not-fat (SNF)	383:440	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	47	theme	lactose	409:415	arg1	nitrogen					453:460	milk urea nitrogen	443:460	milk urea nitrogen (MUN)	443:466	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	47	theme	lactose	409:415	arg1	score					486:490	somatic cell score	473:490	somatic cell score (SCS)	473:496	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	47	theme	lactose	409:415	arg1	yield					376:380	milk yield	371:380	milk yield	371:380	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	47	theme	lactose	409:415	arg1	MUN					463:465	MUN	463:465	MUN	463:465	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	47	theme	lactose	409:415	arg1	SCS					493:495	SCS	493:495	SCS	493:495	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	3	48	theme	7.33	520:523	arg1	mg/dl					601:605	17.6 ± 8.17 mg/dl	589:605	17.6 ± 8.17 mg/dl	589:605	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	48	theme	7.33	520:523	arg1	units					624:628	4.12 ± 2.06 units	612:628	4.12 ± 2.06 units	612:628	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	48	theme	7.33	520:523	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	48	theme	7.33	520:523	arg1	%					586:586	8.72 ± 0.36%	575:586	8.72 ± 0.36%	575:586	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	48	theme	7.33	520:523	arg1	%					572:572	4.89 ± 0.24%	561:572	4.89 ± 0.24%	561:572	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	48	theme	7.33	520:523	arg1	%					558:558	3.02 ± 0.34%	547:558	3.02 ± 0.34%	547:558	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	48	theme	7.33	520:523	arg1	%					544:544	3.54 ± 0.76%	533:544	3.54 ± 0.76%	533:544	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	7	49	theme	studied	1029:1035	arg1	variables					1037:1045	all the other studied variables	1015:1045	all the other studied variables	1015:1045	Except fat and lactose contents, all the other studied variables were significantly influenced by calving season.					
33755808	11	50	theme	lowest	1513:1518	arg1	values					1520:1525	their lowest values	1507:1525	their lowest values between days 35 and 65	1507:1548	Protein and SNF contents and SCS attained their lowest values between days 35 and 65, while fat content between days 65 and 95.					
33755808	4	51	theme	calving	669:675	arg1	year					677:680	calving year	669:680	calving year	669:680	The effects of herd and calving year were found to be significant on all the studied traits.					
33755808	3	52	theme	±	580:580	arg1	%					586:586	8.72 ± 0.36%	575:586	8.72 ± 0.36%	575:586	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	52	theme	±	580:580	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	6	53	theme	later	966:970	arg1	parities					972:979	later parities	966:979	later parities	966:979	Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities.					
33755808	6	54	theme	protein	878:884	arg1	content					886:892	protein content	878:892	protein content	878:892	Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities.					
33755808	2	55	theme	protein	400:406	arg1	contents					383:390	contents	383:390	contents of fat, protein, lactose and solids-not-fat (SNF)	383:440	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	55	theme	protein	400:406	arg1	nitrogen					453:460	milk urea nitrogen	443:460	milk urea nitrogen (MUN)	443:466	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	55	theme	protein	400:406	arg1	score					486:490	somatic cell score	473:490	somatic cell score (SCS)	473:496	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	55	theme	protein	400:406	arg1	yield					376:380	milk yield	371:380	milk yield	371:380	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	55	theme	protein	400:406	arg1	MUN					463:465	MUN	463:465	MUN	463:465	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	55	theme	protein	400:406	arg1	SCS					493:495	SCS	493:495	SCS	493:495	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	56	theme	cell	481:484	arg1	SCS					493:495	SCS	493:495	SCS	493:495	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	56	theme	cell	481:484	arg1	score					486:490	somatic cell score	473:490	somatic cell score (SCS)	473:496	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	5	57	theme	analyzed	795:802	arg1	traits					804:809	all analyzed traits	791:809	all analyzed traits	791:809	Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration.					
33755808	5	58	theme	MUN	834:836	arg1	concentration					838:850	MUN concentration	834:850	MUN concentration	834:850	Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration.					
33755808	8	59	theme	protein	1174:1180	arg1	content					1182:1188	protein content	1174:1188	protein content	1174:1188	Milk yield and MUN were highest for cows calving during humid season, whereas protein content, SNF content, and SCS tended to be higher in dry season.					
33755808	7	60	theme	lactose	997:1003	arg1	contents					1005:1012	fat and lactose contents	989:1012	fat and lactose contents	989:1012	Except fat and lactose contents, all the other studied variables were significantly influenced by calving season.					
33755808	2	61	theme	somatic	473:479	arg1	SCS					493:495	SCS	493:495	SCS	493:495	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	61	theme	somatic	473:479	arg1	score					486:490	somatic cell score	473:490	somatic cell score (SCS)	473:496	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	2	62	theme	day	306:308	arg1	records					310:316	93,815 test day records	294:316	93,815 test day records of 6343 Holstein cows in 162 herds	294:351	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	12	63	from	quality	1700:1706	arg1	Morocco					1711:1717	Morocco	1711:1717	Morocco	1711:1717	It was concluded that these environmental factors should be taken into consideration if the milk yield and quality in Morocco is to be optimized.					
33755808	1	64	theme	Moroccan	190:197	arg1	conditions					199:208	Moroccan conditions	190:208	Moroccan conditions	190:208	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	4	65	theme	year	677:680	arg1	effects					649:655	The effects	645:655	The effects of herd and calving year	645:680	The effects of herd and calving year were found to be significant on all the studied traits.					
33755808	4	65	theme	year	677:680	arg1	significant					699:709	significant	699:709	significant	699:709	The effects of herd and calving year were found to be significant on all the studied traits.					
33755808	3	66	theme	±	538:538	arg1	%					544:544	3.54 ± 0.76%	533:544	3.54 ± 0.76%	533:544	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	66	theme	±	538:538	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	mg/dl					601:605	17.6 ± 8.17 mg/dl	589:605	17.6 ± 8.17 mg/dl	589:605	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	units					624:628	4.12 ± 2.06 units	612:628	4.12 ± 2.06 units	612:628	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	%					586:586	8.72 ± 0.36%	575:586	8.72 ± 0.36%	575:586	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	%					572:572	4.89 ± 0.24%	561:572	4.89 ± 0.24%	561:572	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	%					558:558	3.02 ± 0.34%	547:558	3.02 ± 0.34%	547:558	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	67	theme	±	518:518	arg1	%					544:544	3.54 ± 0.76%	533:544	3.54 ± 0.76%	533:544	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	2	68	theme	test	301:304	arg1	records					310:316	93,815 test day records	294:316	93,815 test day records of 6343 Holstein cows in 162 herds	294:351	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
33755808	1	69	theme	study	107:111	arg1	objectives					88:97	The objectives	84:97	The objectives of this study	84:111	The objectives of this study were to determine milk yield and constituents' levels of Holstein cows under Moroccan conditions and to assess the effects of environmental factors influencing them.					
33755808	0	70	theme	Holstein	58:65	arg1	cows					67:70	Holstein cows	58:70	Holstein cows	58:70	Non-genetic effects on daily milk yield and components of Holstein cows in Morocco.					
33755808	6	71	theme	lactose	934:940	arg1	percentage					942:951	lactose percentage	934:951	lactose percentage	934:951	Milk yield, fat content, protein content, and SCS increased with parity, whereas lactose percentage decreased in later parities.					
33755808	10	72	theme	lactose	1353:1359	arg1	content					1361:1367	lactose content	1353:1367	lactose content	1353:1367	The peaks of milk yield and lactose content occurred between 35 and 65 days, whereas that of MUN between 95 and 125 days after parturition.					
33755808	3	73	theme	±	594:594	arg1	mg/dl					601:605	17.6 ± 8.17 mg/dl	589:605	17.6 ± 8.17 mg/dl	589:605	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	3	73	theme	±	594:594	arg1	kg/day					525:530	25.1 ± 7.33 kg/day	513:530	25.1 ± 7.33 kg/day	513:530	Averages were 25.1 ± 7.33 kg/day, 3.54 ± 0.76%, 3.02 ± 0.34%, 4.89 ± 0.24%, 8.72 ± 0.36%, 17.6 ± 8.17 mg/dl, and 4.12 ± 2.06 units, respectively.					
33755808	12	74	from	yield	1690:1694	arg1	Morocco					1711:1717	Morocco	1711:1717	Morocco	1711:1717	It was concluded that these environmental factors should be taken into consideration if the milk yield and quality in Morocco is to be optimized.					
33755808	5	75	theme	significant	768:778	arg1	effects					780:786	significant effects	768:786	significant effects	768:786	Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration.					
33755808	5	76	contain	have	763:766	arg1	Parity					738:743	Parity	738:743	Parity	738:743	Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration.					
33755808	5	76	contain	have	763:766	arg2	effects					780:786	significant effects	768:786	significant effects	768:786	Parity was determined to have significant effects on all analyzed traits, with the exception of MUN concentration.					
33755808	2	77	theme	cows	335:338	arg1	records					310:316	93,815 test day records	294:316	93,815 test day records of 6343 Holstein cows in 162 herds	294:351	Data including 93,815 test day records of 6343 Holstein cows in 162 herds were analyzed for milk yield, contents of fat, protein, lactose and solids-not-fat (SNF), milk urea nitrogen (MUN), and somatic cell score (SCS).					
32188978	4	0	theme	Bifidobacterium	985:999	arg1	breve					1001:1005	Bifidobacterium breve	985:1005	Bifidobacterium breve	985:1005	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	5	1	theme	B.	1194:1195	arg1	members					1151:1157	members	1151:1157	members of the Bifidobacterium bifidum and B. breve species	1151:1209	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	2	theme	current	485:491	arg1	study					493:497	the current study	481:497	the current study	481:497	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	5	3	dep	bifidum	1182:1188	arg1	the					1162:1164	the	1162:1164	the	1162:1164	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	5	3	dep	bifidum	1182:1188	arg1	species					1203:1209	breve species	1197:1209	breve species	1197:1209	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	6	4	theme	microbiota	1358:1367	arg1	component					1330:1338	the bifidobacterial component	1310:1338	the bifidobacterial component of the human milk microbiota	1310:1367	Altogether, these findings indicate that the bifidobacterial component of the human milk microbiota is not strictly correlated with their ability to metabolize HMOs.					
32188978	5	5	theme	in	1092:1093	arg1	analyses					1113:1120	in silico glycobiome analyses	1092:1120	in silico glycobiome analyses	1092:1120	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	1	6	contain	carry	117:121	arg2	microbiota					131:140	its own microbiota	123:140	its own microbiota	123:140	Human milk is known to carry its own microbiota, of which the precise origin remains obscure.					
32188978	1	6	contain	carry	117:121	arg1	milk					100:103	Human milk	94:103	Human milk	94:103	Human milk is known to carry its own microbiota, of which the precise origin remains obscure.					
32188978	2	7	theme	milk	293:296	arg1	oligosaccharides					329:344	human milk oligosaccharides	318:344	human milk oligosaccharides (HMOs)	318:351	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	7	theme	milk	293:296	arg1	components					298:307	many other milk components	282:307	many other milk components	282:307	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	4	8	theme	Metagenomic	708:718	arg1	data					720:723	Metagenomic data	708:723	Metagenomic data	708:723	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	4	9	theme	heterogeneous	932:944	arg1	mix					946:948	a heterogeneous mix	930:948	a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum	930:1032	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	2	10	theme	other	287:291	arg1	oligosaccharides					329:344	human milk oligosaccharides	318:344	human milk oligosaccharides (HMOs)	318:351	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	10	theme	other	287:291	arg1	components					298:307	many other milk components	282:307	many other milk components	282:307	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	5	11	theme	greatest	1223:1230	arg1	ability					1232:1238	the greatest ability	1219:1238	the greatest ability to degrade and grow on HMOs	1219:1266	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	4	12	theme	bifidobacterial	953:967	arg1	breve					1001:1005	Bifidobacterium breve	985:1005	Bifidobacterium breve	985:1005	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	4	12	theme	bifidobacterial	953:967	arg1	longum					1027:1032	Bifidobacterium longum	1011:1032	Bifidobacterium longum	1011:1032	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	4	12	theme	bifidobacterial	953:967	arg1	species					969:975	bifidobacterial species	953:975	bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum	953:1032	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	3	13	theme	cytometric	685:694	arg1	approaches					696:705	metagenomic and flow cytometric approaches	664:705	metagenomic and flow cytometric approaches	664:705	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	2	14	theme	many	282:285	arg1	oligosaccharides					329:344	human milk oligosaccharides	318:344	human milk oligosaccharides (HMOs)	318:351	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	14	theme	many	282:285	arg1	components					298:307	many other milk components	282:307	many other milk components	282:307	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	1	15	theme	precise	156:162	arg1	origin					164:169	the precise origin	152:169	the precise origin	152:169	Human milk is known to carry its own microbiota, of which the precise origin remains obscure.					
32188978	6	16	theme	milk	1353:1356	arg1	microbiota					1358:1367	the human milk microbiota	1343:1367	the human milk microbiota	1343:1367	Altogether, these findings indicate that the bifidobacterial component of the human milk microbiota is not strictly correlated with their ability to metabolize HMOs.					
32188978	3	17	theme	metagenomic	664:674	arg1	approaches					696:705	metagenomic and flow cytometric approaches	664:705	metagenomic and flow cytometric approaches	664:705	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	0	18	theme	oligosaccharide	44:58	arg1	composition					60:70	human milk oligosaccharide composition	33:70	human milk oligosaccharide composition	33:70	Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers.					
32188978	1	19	theme	Human	94:98	arg1	milk					100:103	Human milk	94:103	Human milk	94:103	Human milk is known to carry its own microbiota, of which the precise origin remains obscure.					
32188978	2	20	theme	metabolizable	367:379	arg1	bifidobacteria					425:438	bifidobacteria	425:438	bifidobacteria	425:438	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	20	theme	metabolizable	367:379	arg1	substrates					381:390	metabolizable substrates	367:390	metabolizable substrates	367:390	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	20	theme	metabolizable	367:379	arg1	transfer					270:277	the transfer	266:277	the transfer	266:277	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	5	21	from	assays	1064:1069	arg1	HMOs					1074:1077	HMOs	1074:1077	HMOs coupled with in silico glycobiome analyses	1074:1120	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	22	theme	human	537:541	arg1	samples					548:554	249 human milk samples	533:554	249 human milk samples	533:554	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	5	23	theme	glycobiome	1102:1111	arg1	analyses					1113:1120	in silico glycobiome analyses	1092:1120	in silico glycobiome analyses	1092:1120	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	5	24	theme	bifidum	1182:1188	arg1	members					1151:1157	members	1151:1157	members of the Bifidobacterium bifidum and B. breve species	1151:1209	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	25	theme	milk	543:546	arg1	samples					548:554	249 human milk samples	533:554	249 human milk samples	533:554	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	0	26	theme	human	33:37	arg1	composition					60:70	human milk oligosaccharide composition	33:70	human milk oligosaccharide composition	33:70	Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers.					
32188978	6	27	theme	human	1347:1351	arg1	microbiota					1358:1367	the human milk microbiota	1343:1367	the human milk microbiota	1343:1367	Altogether, these findings indicate that the bifidobacterial component of the human milk microbiota is not strictly correlated with their ability to metabolize HMOs.					
32188978	5	28	theme	in	1048:1049	arg1	assays					1064:1069	in vitro growth assays	1048:1069	in vitro growth assays on HMOs coupled with in silico glycobiome analyses	1048:1120	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	29	theme	approaches	696:705	arg1	combination					649:659	a combination	647:659	a combination of metagenomic and flow cytometric approaches	647:705	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	2	30	theme	components	298:307	arg1	bifidobacteria					425:438	bifidobacteria	425:438	bifidobacteria	425:438	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	30	theme	components	298:307	arg1	substrates					381:390	metabolizable substrates	367:390	metabolizable substrates	367:390	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	30	theme	components	298:307	arg1	transfer					270:277	the transfer	266:277	the transfer	266:277	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	31	theme	intestinal	460:469	arg1	tract					471:475	infant intestinal tract	453:475	infant intestinal tract	453:475	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	5	32	theme	breve	1197:1201	arg1	species					1203:1209	breve species	1197:1209	breve species	1197:1209	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	33	theme	members	604:610	arg1	abundance					591:599	the abundance	587:599	the abundance of members of the Bifidobacterium genus	587:639	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	2	34	theme	particular	396:405	arg1	bacteria					407:414	particular bacteria	396:414	particular bacteria	396:414	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	0	35	theme	milk	39:42	arg1	composition					60:70	human milk oligosaccharide composition	33:70	human milk oligosaccharide composition	33:70	Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers.					
32188978	2	36	theme	infant	453:458	arg1	tract					471:475	infant intestinal tract	453:475	infant intestinal tract	453:475	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	37	theme	microorganisms	240:253	arg1	transmission					224:235	mother-to-baby transmission	209:235	mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract	209:475	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	6	38	theme	bifidobacterial	1314:1328	arg1	component					1330:1338	the bifidobacterial component	1310:1338	the bifidobacterial component of the human milk microbiota	1310:1367	Altogether, these findings indicate that the bifidobacterial component of the human milk microbiota is not strictly correlated with their ability to metabolize HMOs.					
32188978	4	39	theme	species	969:975	arg1	mix					946:948	a heterogeneous mix	930:948	a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum	930:1032	Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum.					
32188978	3	40	theme	samples	548:554	arg1	composition					518:528	the HMO composition	510:528	the HMO composition of 249 human milk samples	510:554	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	2	41	theme	mother-to-baby	209:222	arg1	transmission					224:235	mother-to-baby transmission	209:235	mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract	209:475	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	5	42	theme	growth	1057:1062	arg1	assays					1064:1069	in vitro growth assays	1048:1069	in vitro growth assays on HMOs coupled with in silico glycobiome analyses	1048:1120	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	43	theme	genus	635:639	arg1	members					604:610	members	604:610	members of the Bifidobacterium genus	604:639	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	1	44	theme	own	127:129	arg1	microbiota					131:140	its own microbiota	123:140	its own microbiota	123:140	Human milk is known to carry its own microbiota, of which the precise origin remains obscure.					
32188978	3	45	theme	HMO	514:516	arg1	composition					518:528	the HMO composition	510:528	the HMO composition of 249 human milk samples	510:554	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	2	46	theme	human	318:322	arg1	oligosaccharides					329:344	human milk oligosaccharides	318:344	human milk oligosaccharides (HMOs)	318:351	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	46	theme	human	318:322	arg1	HMOs					347:350	HMOs	347:350	HMOs	347:350	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	47	theme	milk	324:327	arg1	oligosaccharides					329:344	human milk oligosaccharides	318:344	human milk oligosaccharides (HMOs)	318:351	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	2	47	theme	milk	324:327	arg1	HMOs					347:350	HMOs	347:350	HMOs	347:350	Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract.					
32188978	0	48	theme	mothers	85:91	arg1	composition					60:70	human milk oligosaccharide composition	33:70	human milk oligosaccharide composition	33:70	Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers.					
32188978	0	48	theme	mothers	85:91	arg1	bifidobacteria					14:27	bifidobacteria	14:27	bifidobacteria	14:27	Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers.					
32188978	5	49	dep	in	1048:1049	arg1	vitro					1051:1055	vitro	1051:1055	vitro	1051:1055	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32188978	3	50	theme	Bifidobacterium	619:633	arg1	genus					635:639	the Bifidobacterium genus	615:639	the Bifidobacterium genus	615:639	In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches.					
32188978	0	51	theme	lactating	75:83	arg1	mothers					85:91	lactating mothers	75:91	lactating mothers	75:91	Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers.					
32188978	5	52	dep	in	1092:1093	arg1	silico					1095:1100	silico	1095:1100	silico	1095:1100	Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs.					
32805972	0	0	theme	Purple	81:86	arg1	Potatoes					94:101	Purple Sweet Potatoes	81:101	Purple Sweet Potatoes	81:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	6	1	theme	dielectric	902:911	arg1	loss					913:916	the dielectric loss	898:916	the dielectric loss of the purees	898:930	Addition of both salt and syrup decreased the dielectric constant but increased the dielectric loss of the purees.					
32805972	9	2	theme	deformation	1165:1175	arg1	pattern					1177:1183	A desirable deformation pattern	1153:1183	A desirable deformation pattern	1153:1183	A desirable deformation pattern could also be achieved by manipulating the infill parameters.					
32805972	10	3	theme	planar	1281:1286	arg1	flowers					1288:1294	two-dimensional planar flowers	1265:1294	two-dimensional planar flowers	1265:1294	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	2	4	theme	microwave	467:475	arg1	dehydration					477:487	microwave dehydration	467:487	microwave dehydration	467:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	2	5	dep	formulations	438:449	arg1	triggered					454:462	triggered	454:462	triggered by microwave dehydration	454:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	11	6	theme	spontaneous	1437:1447	arg1	change					1455:1460	spontaneous shape change	1437:1460	spontaneous shape change of a 3D printed starch-based product	1437:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	10	7	theme	two-dimensional	1265:1279	arg1	flowers					1288:1294	two-dimensional planar flowers	1265:1294	two-dimensional planar flowers	1265:1294	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	1	8	theme	postprinting	246:257	arg1	stimulus					259:266	postprinting stimulus	246:266	postprinting stimulus	246:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	11	9	theme	shape	1449:1453	arg1	change					1455:1460	spontaneous shape change	1437:1460	spontaneous shape change of a 3D printed starch-based product	1437:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	0	10	from	Investigation	0:12	arg1	Change					35:40	Spontaneous Shape Change	17:40	Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes	17:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	6	11	theme	syrup	844:848	arg1	Addition					818:825	Addition	818:825	Addition of both salt and syrup	818:848	Addition of both salt and syrup decreased the dielectric constant but increased the dielectric loss of the purees.					
32805972	3	12	theme	distribution	541:552	arg1	behavior					554:561	water distribution behavior	535:561	water distribution behavior	535:561	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	10	13	theme	parameters	1377:1386	arg1	result					1340:1345	a result	1338:1345	a result of varying the aforementioned parameters	1338:1386	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	0	14	theme	Sweet	88:92	arg1	Potatoes					94:101	Purple Sweet Potatoes	81:101	Purple Sweet Potatoes	81:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	7	15	theme	Increased	933:941	arg1	power					953:957	Increased microwave power	933:957	Increased microwave power	933:957	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	10	16	theme	3D	1317:1318	arg1	configurations					1320:1333	3D configurations	1317:1333	3D configurations as a result of varying the aforementioned parameters	1317:1386	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	11	17	theme	3D	1467:1468	arg1	product					1491:1497	a 3D printed starch-based product	1465:1497	a 3D printed starch-based product	1465:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	7	18	theme	samples	1087:1093	arg1	degree					1065:1070	the maximum deformation degree	1041:1070	the maximum deformation degree of the printed samples	1041:1093	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	2	19	theme	purple	398:403	arg1	purees					418:423	3D printed purple sweet potato purees	387:423	3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	387:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	11	20	theme	printed	1470:1476	arg1	product					1491:1497	a 3D printed starch-based product	1465:1497	a 3D printed starch-based product	1465:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	1	21	theme	three-dimensional	162:178	arg1	structures					198:207	three-dimensional (3D) printed food structures	162:207	three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus	162:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	5	22	theme	syrup	782:786	arg1	Addition					761:768	Addition	761:768	Addition of fructose syrup	761:786	Addition of fructose syrup resulted in opposite results.					
32805972	6	23	theme	dielectric	864:873	arg1	constant					875:882	the dielectric constant	860:882	the dielectric constant	860:882	Addition of both salt and syrup decreased the dielectric constant but increased the dielectric loss of the purees.					
32805972	11	24	theme	four-dimensional	1550:1565	arg1	printing					1572:1579	four-dimensional food printing	1550:1579	four-dimensional food printing	1550:1579	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	4	25	theme	salt	636:639	arg1	Addition					624:631	Addition	624:631	Addition of salt	624:639	Addition of salt reduced the viscosity, storage modulus, loss modulus, and yield stress but increased the relaxation time of the purees.					
32805972	3	26	theme	water	535:539	arg1	behavior					554:561	water distribution behavior	535:561	water distribution behavior	535:561	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	10	27	theme	butterflies	1300:1310	arg1	Transformation					1247:1260	Transformation	1247:1260	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters	1247:1386	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	2	28	theme	different	428:436	arg1	formulations					438:449	different formulations	428:449	different formulations as triggered by microwave dehydration	428:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	0	29	from	Change	35:40	arg1	Potatoes					94:101	Purple Sweet Potatoes	81:101	Purple Sweet Potatoes	81:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	8	30	theme	maximum	1125:1131	arg1	degree					1145:1150	the maximum deformation degree	1121:1150	the maximum deformation degree	1121:1150	The syrup also decreased the maximum deformation degree.					
32805972	3	31	theme	dielectric	568:577	arg1	properties					579:588	dielectric properties	568:588	dielectric properties	568:588	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	2	32	theme	shape	371:375	arg1	change					377:382	the spontaneous shape change	355:382	the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	355:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	7	33	theme	printed	1079:1085	arg1	samples					1087:1093	the printed samples	1075:1093	the printed samples	1075:1093	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	2	34	theme	spontaneous	359:369	arg1	change					377:382	the spontaneous shape change	355:382	the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	355:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	0	35	theme	Shape	29:33	arg1	Change					35:40	Spontaneous Shape Change	17:40	Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes	17:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	0	36	theme	Microwave	117:125	arg1	Dehydration					127:137	Microwave Dehydration	117:137	Microwave Dehydration	117:137	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	7	37	theme	microwave	943:951	arg1	power					953:957	Increased microwave power	933:957	Increased microwave power	933:957	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	5	38	theme	fructose	773:780	arg1	syrup					782:786	fructose syrup	773:786	fructose syrup	773:786	Addition of fructose syrup resulted in opposite results.					
32805972	2	39	theme	sweet	405:409	arg1	purees					418:423	3D printed purple sweet potato purees	387:423	3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	387:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	0	40	theme	Spontaneous	17:27	arg1	Change					35:40	Spontaneous Shape Change	17:40	Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes	17:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	11	41	theme	food	1567:1570	arg1	printing					1572:1579	four-dimensional food printing	1550:1579	four-dimensional food printing	1550:1579	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	9	42	theme	infill	1228:1233	arg1	parameters					1235:1244	the infill parameters	1224:1244	the infill parameters	1224:1244	A desirable deformation pattern could also be achieved by manipulating the infill parameters.					
32805972	4	43	theme	relaxation	730:739	arg1	time					741:744	the relaxation time	726:744	the relaxation time of the purees	726:758	Addition of salt reduced the viscosity, storage modulus, loss modulus, and yield stress but increased the relaxation time of the purees.					
32805972	11	44	theme	starch-based	1478:1489	arg1	product					1491:1497	a 3D printed starch-based product	1465:1497	a 3D printed starch-based product	1465:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	1	45	theme	food	193:196	arg1	structures					198:207	three-dimensional (3D) printed food structures	162:207	three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus	162:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	11	46	theme	product	1491:1497	arg1	change					1455:1460	spontaneous shape change	1437:1460	spontaneous shape change of a 3D printed starch-based product	1437:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	1	47	theme	research	282:289	arg1	evolution					149:157	The time evolution	140:157	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus	140:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	1	47	theme	research	282:289	arg1	area					274:277	an area	271:277	an area of research that has recently received increasing attention	271:337	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	7	48	theme	maximum	1045:1051	arg1	degree					1065:1070	the maximum deformation degree	1041:1070	the maximum deformation degree of the printed samples	1041:1093	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	2	49	theme	printed	390:396	arg1	purees					418:423	3D printed purple sweet potato purees	387:423	3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	387:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	3	50	theme	purees	597:602	arg1	behavior					554:561	water distribution behavior	535:561	water distribution behavior	535:561	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	3	50	theme	purees	597:602	arg1	properties					579:588	dielectric properties	568:588	dielectric properties	568:588	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	3	50	theme	purees	597:602	arg1	properties					523:532	The rheological properties	507:532	The rheological properties	507:532	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	4	51	theme	storage	664:670	arg1	modulus					672:678	storage modulus	664:678	storage modulus	664:678	Addition of salt reduced the viscosity, storage modulus, loss modulus, and yield stress but increased the relaxation time of the purees.					
32805972	1	52	theme	structures	198:207	arg1	evolution					149:157	The time evolution	140:157	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus	140:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	1	52	theme	structures	198:207	arg1	area					274:277	an area	271:277	an area of research that has recently received increasing attention	271:337	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	4	53	theme	loss	681:684	arg1	modulus					686:692	loss modulus	681:692	loss modulus	681:692	Addition of salt reduced the viscosity, storage modulus, loss modulus, and yield stress but increased the relaxation time of the purees.					
32805972	7	54	theme	deformation	1053:1063	arg1	degree					1065:1070	the maximum deformation degree	1041:1070	the maximum deformation degree of the printed samples	1041:1093	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	8	55	theme	deformation	1133:1143	arg1	degree					1145:1150	the maximum deformation degree	1121:1150	the maximum deformation degree	1121:1150	The syrup also decreased the maximum deformation degree.					
32805972	2	56	theme	purees	418:423	arg1	change					377:382	the spontaneous shape change	355:382	the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	355:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	7	57	theme	dehydration	999:1009	arg1	rates					990:994	the rates	986:994	the rates of dehydration and deformation	986:1025	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	2	58	theme	3D	387:388	arg1	purees					418:423	3D printed purple sweet potato purees	387:423	3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	387:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	0	59	theme	Printed	48:54	arg1	Purees					69:74	4D Printed Starch-Based Purees	45:74	4D Printed Starch-Based Purees from Purple Sweet Potatoes	45:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	6	60	theme	purees	925:930	arg1	loss					913:916	the dielectric loss	898:916	the dielectric loss of the purees	898:930	Addition of both salt and syrup decreased the dielectric constant but increased the dielectric loss of the purees.					
32805972	6	61	theme	salt	835:838	arg1	Addition					818:825	Addition	818:825	Addition of both salt and syrup	818:848	Addition of both salt and syrup decreased the dielectric constant but increased the dielectric loss of the purees.					
32805972	1	62	dep	three-dimensional	162:178	arg1	3D					181:182	3D	181:182	3D	181:182	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	4	63	theme	yield	699:703	arg1	stress					705:710	yield stress	699:710	yield stress	699:710	Addition of salt reduced the viscosity, storage modulus, loss modulus, and yield stress but increased the relaxation time of the purees.					
32805972	2	64	theme	formulations	438:449	arg1	purees					418:423	3D printed purple sweet potato purees	387:423	3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	387:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	5	65	theme	opposite	800:807	arg1	results					809:815	opposite results	800:815	opposite results	800:815	Addition of fructose syrup resulted in opposite results.					
32805972	10	66	theme	varying	1350:1356	arg1	parameters					1377:1386	the aforementioned parameters	1358:1386	varying the aforementioned parameters	1350:1386	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	7	67	theme	deformation	1015:1025	arg1	rates					990:994	the rates	986:994	the rates of dehydration and deformation	986:1025	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	11	68	theme	printing	1572:1579	arg1	application					1535:1545	further application	1527:1545	further application of four-dimensional food printing	1527:1579	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	11	69	theme	proposed	1408:1415	arg1	technique					1417:1425	The proposed technique	1404:1425	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product	1404:1497	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	9	70	theme	desirable	1155:1163	arg1	pattern					1177:1183	A desirable deformation pattern	1153:1183	A desirable deformation pattern	1153:1183	A desirable deformation pattern could also be achieved by manipulating the infill parameters.					
32805972	4	71	theme	purees	753:758	arg1	time					741:744	the relaxation time	726:744	the relaxation time of the purees	726:758	Addition of salt reduced the viscosity, storage modulus, loss modulus, and yield stress but increased the relaxation time of the purees.					
32805972	0	72	from	Potatoes	94:101	arg1	Purees					69:74	4D Printed Starch-Based Purees	45:74	4D Printed Starch-Based Purees from Purple Sweet Potatoes	45:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	0	72	from	Potatoes	94:101	arg1	Change					35:40	Spontaneous Shape Change	17:40	Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes	17:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	10	73	theme	aforementioned	1362:1375	arg1	parameters					1377:1386	the aforementioned parameters	1358:1386	varying the aforementioned parameters	1350:1386	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
32805972	0	74	theme	Purees	69:74	arg1	Change					35:40	Spontaneous Shape Change	17:40	Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes	17:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	1	75	theme	printed	185:191	arg1	structures					198:207	three-dimensional (3D) printed food structures	162:207	three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus	162:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	3	76	theme	rheological	511:521	arg1	properties					523:532	The rheological properties	507:532	The rheological properties	507:532	The rheological properties, water distribution behavior, and dielectric properties of the purees were first studied.					
32805972	11	77	theme	further	1527:1533	arg1	application					1535:1545	further application	1527:1545	further application of four-dimensional food printing	1527:1579	The proposed technique to induce spontaneous shape change of a 3D printed starch-based product should lay a foundation for further application of four-dimensional food printing.					
32805972	0	78	theme	Starch-Based	56:67	arg1	Purees					69:74	4D Printed Starch-Based Purees	45:74	4D Printed Starch-Based Purees from Purple Sweet Potatoes	45:101	Investigation on Spontaneous Shape Change of 4D Printed Starch-Based Purees from Purple Sweet Potatoes As Induced by Microwave Dehydration.					
32805972	1	79	theme	increasing	318:327	arg1	attention					329:337	increasing attention	318:337	increasing attention	318:337	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	2	80	theme	potato	411:416	arg1	purees					418:423	3D printed purple sweet potato purees	387:423	3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration	387:487	In this study, the spontaneous shape change of 3D printed purple sweet potato purees of different formulations as triggered by microwave dehydration was investigated.					
32805972	7	81	theme	salt	963:966	arg1	content					968:974	salt content	963:974	salt content	963:974	Increased microwave power and salt content increased the rates of dehydration and deformation but decreased the maximum deformation degree of the printed samples.					
32805972	1	82	theme	time	144:147	arg1	evolution					149:157	The time evolution	140:157	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus	140:266	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	1	82	theme	time	144:147	arg1	area					274:277	an area	271:277	an area of research that has recently received increasing attention	271:337	The time evolution of three-dimensional (3D) printed food structures as affected by their composition and postprinting stimulus is an area of research that has recently received increasing attention.					
32805972	10	83	theme	flowers	1288:1294	arg1	Transformation					1247:1260	Transformation	1247:1260	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters	1247:1386	Transformation of two-dimensional planar flowers and butterflies into 3D configurations as a result of varying the aforementioned parameters is illustrated.					
34051250	8	0	theme	pasting	1011:1017	arg1	changes					1027:1033	the pasting profile changes	1007:1033	the pasting profile changes	1007:1033	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	5	1	theme	hexagonal	567:575	arg1	nanocrystal					594:604	hexagonal and orthorhombic nanocrystal	567:604	hexagonal and orthorhombic nanocrystal	567:604	C-type starch formed by hexagonal and orthorhombic nanocrystal was completely indexed.					
34051250	0	2	theme	properties	57:66	arg1	Study					0:4	Study	0:4	Study of morphological, structural, thermal, and pasting properties of flour	0:75	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	8	3	theme	Scanning	851:858	arg1	microscopy					869:878	Scanning electron microscopy	851:878	Scanning electron microscopy of the lyophilized samples along pasting profile	851:927	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	3	4	theme	semispherical	405:417	arg1	morphologies					419:430	lenticular, elliptical, and semispherical morphologies	377:430	lenticular, elliptical, and semispherical morphologies	377:430	This starch exhibits lenticular, elliptical, and semispherical morphologies.					
34051250	1	5	theme	structural	214:223	arg1	properties					238:247	thermal, structural, and pasting properties	205:247	thermal, structural, and pasting properties of isolated plantain starch	205:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	1	5	theme	structural	214:223	arg1	composition					180:190	the mineral composition	168:190	the mineral composition	168:190	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	6	6	theme	Magnesium	706:714	arg1	Ca2.71Mg0.29					727:738	Ca2.71Mg0.29(PO4)2	727:744	Ca2.71Mg0.29(PO4)2	727:744	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	6	6	theme	Magnesium	706:714	arg1	Phosphate					716:724	Calcium Magnesium Phosphate	698:724	Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2)	698:745	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	5	7	theme	orthorhombic	581:592	arg1	nanocrystal					594:604	hexagonal and orthorhombic nanocrystal	567:604	hexagonal and orthorhombic nanocrystal	567:604	C-type starch formed by hexagonal and orthorhombic nanocrystal was completely indexed.					
34051250	4	8	theme	hexagonal	505:513	arg1	events					449:454	Two endothermal events	433:454	Two endothermal events present in the thermogram	433:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	8	theme	hexagonal	505:513	arg1	solvation					532:540	the hexagonal and orthorhombic solvation	501:540	the hexagonal and orthorhombic solvation	501:540	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	0	9	theme	flour	71:75	arg1	properties					57:66	morphological, structural, thermal, and pasting properties	9:66	morphological, structural, thermal, and pasting properties of flour	9:75	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	5	10	theme	C-type	543:548	arg1	starch					550:555	C-type starch	543:555	C-type starch formed by hexagonal and orthorhombic nanocrystal	543:604	C-type starch formed by hexagonal and orthorhombic nanocrystal was completely indexed.					
34051250	2	11	from	rich	297:300	arg1	K					305:305	K	305:305	K	305:305	Plantain starch is rich in K, and other ions as Mg, Ca, P, and Si were found.					
34051250	6	12	theme	Phosphate	716:724	arg1	presence					657:664	the presence	653:664	the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2)	653:771	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	6	13	theme	ash	634:636	arg1	content					638:644	The ash content	630:644	The ash content	630:644	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	8	14	theme	profile	1019:1025	arg1	changes					1027:1033	the pasting profile changes	1007:1033	the pasting profile changes	1007:1033	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	2	15	from	K	305:305	arg1	rich					297:300	rich	297:300	rich	297:300	Plantain starch is rich in K, and other ions as Mg, Ca, P, and Si were found.					
34051250	2	16	theme	Plantain	278:285	arg1	starch					287:292	Plantain starch	278:292	Plantain starch	278:292	Plantain starch is rich in K, and other ions as Mg, Ca, P, and Si were found.					
34051250	8	17	theme	der	961:963	arg1	forces					971:976	the shear and van der Walls forces	943:976	forces	971:976	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	4	18	from	thermogram	471:480	arg1	present					456:462	present	456:462	present	456:462	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	1	19	theme	pasting	230:236	arg1	properties					238:247	thermal, structural, and pasting properties	205:247	thermal, structural, and pasting properties of isolated plantain starch	205:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	1	19	theme	pasting	230:236	arg1	composition					180:190	the mineral composition	168:190	the mineral composition	168:190	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	6	20	theme	Calcium	698:704	arg1	Ca2.71Mg0.29					727:738	Ca2.71Mg0.29(PO4)2	727:744	Ca2.71Mg0.29(PO4)2	727:744	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	6	20	theme	Calcium	698:704	arg1	Phosphate					716:724	Calcium Magnesium Phosphate	698:724	Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2)	698:745	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	8	21	theme	samples	899:905	arg1	microscopy					869:878	Scanning electron microscopy	851:878	Scanning electron microscopy of the lyophilized samples along pasting profile	851:927	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	7	22	theme	starch	802:807	arg1	profile					786:792	The pasting profile	774:792	The pasting profile of this starch	774:807	The pasting profile of this starch behaves between a custard and a hydrogel.					
34051250	0	23	theme	unripe	102:107	arg1	plantain					109:116	unripe plantain	102:116	unripe plantain (Musa paradisiaca)	102:135	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	0	23	theme	unripe	102:107	arg1	paradisiaca					124:134	Musa paradisiaca	119:134	Musa paradisiaca	119:134	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	8	24	theme	electron	860:867	arg1	microscopy					869:878	Scanning electron microscopy	851:878	Scanning electron microscopy of the lyophilized samples along pasting profile	851:927	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	0	25	theme	morphological	9:21	arg1	properties					57:66	morphological, structural, thermal, and pasting properties	9:66	morphological, structural, thermal, and pasting properties of flour	9:75	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	8	26	theme	van	957:959	arg1	forces					971:976	the shear and van der Walls forces	943:976	forces	971:976	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	4	27	attach	present	456:462	arg2	solvation					532:540	the hexagonal and orthorhombic solvation	501:540	the hexagonal and orthorhombic solvation	501:540	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	27	attach	present	456:462	arg1	thermogram					471:480	the thermogram	467:480	the thermogram	467:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	27	attach	present	456:462	arg2	events					449:454	Two endothermal events	433:454	Two endothermal events present in the thermogram	433:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	28	from	present	456:462	arg1	thermogram					471:480	the thermogram	467:480	the thermogram	467:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	1	29	theme	isolated	252:259	arg1	starch					270:275	isolated plantain starch	252:275	isolated plantain starch	252:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	6	30	theme	oxide	760:764	arg1	presence					657:664	the presence	653:664	the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2)	653:771	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	1	31	theme	mineral	172:178	arg1	morphology					193:202	morphology	193:202	morphology	193:202	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	1	31	theme	mineral	172:178	arg1	properties					238:247	thermal, structural, and pasting properties	205:247	thermal, structural, and pasting properties of isolated plantain starch	205:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	1	31	theme	mineral	172:178	arg1	composition					180:190	the mineral composition	168:190	the mineral composition	168:190	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	0	32	theme	structural	24:33	arg1	properties					57:66	morphological, structural, thermal, and pasting properties	9:66	morphological, structural, thermal, and pasting properties of flour	9:75	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	1	33	theme	plantain	261:268	arg1	starch					270:275	isolated plantain starch	252:275	isolated plantain starch	252:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	8	34	theme	Walls	965:969	arg1	forces					971:976	the shear and van der Walls forces	943:976	forces	971:976	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	6	35	theme	silicon	752:758	arg1	SiO2					767:770	SiO2	767:770	SiO2	767:770	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	6	35	theme	silicon	752:758	arg1	oxide					760:764	silicon oxide	752:764	silicon oxide (SiO2)	752:771	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	1	36	theme	starch	270:275	arg1	properties					238:247	thermal, structural, and pasting properties	205:247	thermal, structural, and pasting properties of isolated plantain starch	205:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	1	36	theme	starch	270:275	arg1	composition					180:190	the mineral composition	168:190	the mineral composition	168:190	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	8	37	theme	slurry	982:987	arg1	morphology					989:998	slurry morphology	982:998	slurry morphology	982:998	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	4	38	theme	endothermal	437:447	arg1	events					449:454	Two endothermal events	433:454	Two endothermal events present in the thermogram	433:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	38	theme	endothermal	437:447	arg1	solvation					532:540	the hexagonal and orthorhombic solvation	501:540	the hexagonal and orthorhombic solvation	501:540	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	3	39	theme	elliptical	389:398	arg1	morphologies					419:430	lenticular, elliptical, and semispherical morphologies	377:430	lenticular, elliptical, and semispherical morphologies	377:430	This starch exhibits lenticular, elliptical, and semispherical morphologies.					
34051250	0	40	theme	thermal	36:42	arg1	properties					57:66	morphological, structural, thermal, and pasting properties	9:66	morphological, structural, thermal, and pasting properties of flour	9:75	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	4	41	theme	present	456:462	arg1	events					449:454	Two endothermal events	433:454	Two endothermal events present in the thermogram	433:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	41	theme	present	456:462	arg1	solvation					532:540	the hexagonal and orthorhombic solvation	501:540	the hexagonal and orthorhombic solvation	501:540	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	0	42	theme	Musa	119:122	arg1	plantain					109:116	unripe plantain	102:116	unripe plantain (Musa paradisiaca)	102:135	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	0	42	theme	Musa	119:122	arg1	paradisiaca					124:134	Musa paradisiaca	119:134	Musa paradisiaca	119:134	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	8	43	theme	pasting	913:919	arg1	profile					921:927	pasting profile	913:927	pasting profile	913:927	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	8	44	theme	lyophilized	887:897	arg1	samples					899:905	the lyophilized samples	883:905	the lyophilized samples	883:905	Scanning electron microscopy of the lyophilized samples along pasting profile confirms that the shear and van der Walls forces and slurry morphology govern the pasting profile changes.					
34051250	6	45	theme	phosphate	677:685	arg1	presence					657:664	the presence	653:664	the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2)	653:771	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	3	46	theme	lenticular	377:386	arg1	morphologies					419:430	lenticular, elliptical, and semispherical morphologies	377:430	lenticular, elliptical, and semispherical morphologies	377:430	This starch exhibits lenticular, elliptical, and semispherical morphologies.					
34051250	4	47	theme	orthorhombic	519:530	arg1	events					449:454	Two endothermal events	433:454	Two endothermal events present in the thermogram	433:480	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	4	47	theme	orthorhombic	519:530	arg1	solvation					532:540	the hexagonal and orthorhombic solvation	501:540	the hexagonal and orthorhombic solvation	501:540	Two endothermal events present in the thermogram were identified as the hexagonal and orthorhombic solvation.					
34051250	6	48	theme	calcium	669:675	arg1	KCaP2O7					688:694	KCaP2O7	688:694	KCaP2O7	688:694	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	6	48	theme	calcium	669:675	arg1	phosphate					677:685	calcium phosphate	669:685	calcium phosphate (KCaP2O7)	669:695	The ash content showed the presence of calcium phosphate (KCaP2O7), Calcium Magnesium Phosphate (Ca2.71Mg0.29(PO4)2), and silicon oxide (SiO2).					
34051250	7	49	theme	pasting	778:784	arg1	profile					786:792	The pasting profile	774:792	The pasting profile of this starch	774:807	The pasting profile of this starch behaves between a custard and a hydrogel.					
34051250	0	50	theme	pasting	49:55	arg1	properties					57:66	morphological, structural, thermal, and pasting properties	9:66	morphological, structural, thermal, and pasting properties of flour	9:75	Study of morphological, structural, thermal, and pasting properties of flour and isolated starch from unripe plantain (Musa paradisiaca).					
34051250	2	51	theme	other	312:316	arg1	ions					318:321	other ions	312:321	other ions as Mg, Ca, P, and Si	312:342	Plantain starch is rich in K, and other ions as Mg, Ca, P, and Si were found.					
34051250	1	52	theme	thermal	205:211	arg1	properties					238:247	thermal, structural, and pasting properties	205:247	thermal, structural, and pasting properties of isolated plantain starch	205:275	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
34051250	1	52	theme	thermal	205:211	arg1	composition					180:190	the mineral composition	168:190	the mineral composition	168:190	This work focused on studying the mineral composition, morphology, thermal, structural, and pasting properties of isolated plantain starch.					
32483971	7	0	theme	Glycan	859:864	arg1	profiles					866:873	Glycan profiles	859:873	Glycan profiles	859:873	Glycan profiles and isomeric quantitations were assessed by liquid chromatography-mass spectrometry analysis and compared with healthy controls.					
32483971	8	1	theme	N-glycan	1007:1014	arg1	candidates					1026:1035	24 N-glycan biomarker candidates	1004:1035	24 N-glycan biomarker candidates	1004:1035	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	0	2	with	Patients	29:36	arg1	Restless					51:58	Primary Restless	43:58	Primary Restless	43:58	Serum Glycomics Profiling of Patients with Primary Restless Legs Syndrome Using LC-MS/MS.					
32483971	9	3	theme	p	1350:1350	arg1	profile					1341:1347	the expression profile	1326:1347	the expression profile (p < 0.001)	1326:1359	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	9	3	theme	p	1350:1350	arg1	<					1352:1352	p < 0.001	1350:1358	p < 0.001	1350:1358	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	9	4	theme	expression	1330:1339	arg1	profile					1341:1347	the expression profile	1326:1347	the expression profile (p < 0.001)	1326:1359	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	9	4	theme	expression	1330:1339	arg1	<					1352:1352	p < 0.001	1350:1358	p < 0.001	1350:1358	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	5	5	theme	specific	593:600	arg1	changes					610:616	specific glycome changes	593:616	specific glycome changes	593:616	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	7	6	theme	liquid	919:924	arg1	analysis					959:966	liquid chromatography-mass spectrometry analysis	919:966	liquid chromatography-mass spectrometry analysis	919:966	Glycan profiles and isomeric quantitations were assessed by liquid chromatography-mass spectrometry analysis and compared with healthy controls.					
32483971	1	7	theme	Willis-Ekbom	134:145	arg1	disease					147:153	Willis-Ekbom disease	134:153	Willis-Ekbom disease	134:153	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	10	8	theme	Benjamini-Hochberg	1456:1473	arg1	correction					1492:1501	the Benjamini-Hochberg multiple testing correction	1452:1501	the Benjamini-Hochberg multiple testing correction	1452:1501	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	13	9	theme	specific	1782:1789	arg1	traits					1799:1804	specific glycome traits	1782:1804	specific glycome traits in individuals with RLS	1782:1828	These results demonstrate that it is possible to detect specific glycome traits in individuals with RLS.					
32483971	6	10	theme	patients	849:856	arg1	serum					836:840	the blood serum	826:840	the blood serum of RLS patients	826:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	5	11	theme	glycome	602:608	arg1	changes					610:616	specific glycome changes	593:616	specific glycome changes	593:616	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	5	12	theme	underlying	539:548	arg1	mechanisms					562:571	the underlying biochemical mechanisms	535:571	the underlying biochemical mechanisms of RLS	535:578	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	10	13	theme	isomeric	1378:1385	arg1	structures					1387:1396	23 isomeric structures	1375:1396	23 isomeric structures in the RLS cohorts	1375:1415	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	13	14	theme	glycome	1791:1797	arg1	traits					1799:1804	specific glycome traits	1782:1804	specific glycome traits in individuals with RLS	1782:1828	These results demonstrate that it is possible to detect specific glycome traits in individuals with RLS.					
32483971	6	15	theme	RLS	845:847	arg1	patients					849:856	RLS patients	845:856	RLS patients	845:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	8	16	theme	biomarker	1016:1024	arg1	candidates					1026:1035	24 N-glycan biomarker candidates	1004:1035	24 N-glycan biomarker candidates	1004:1035	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	5	17	theme	biochemical	550:560	arg1	mechanisms					562:571	the underlying biochemical mechanisms	535:571	the underlying biochemical mechanisms of RLS	535:578	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	14	18	theme	therapeutic	2038:2048	arg1	interventions					2050:2062	novel targeted therapeutic interventions	2023:2062	novel targeted therapeutic interventions	2023:2062	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	11	19	theme	RLS	1604:1606	arg1	cohort					1608:1613	the RLS cohort	1600:1613	the RLS cohort	1600:1613	HexNAc4Hex5Fuc1NeuAc2 (4512-3) and HexNAc6Hex7NeuAc3 (6703-1) (p < 0.001) were downexpressed in the RLS cohort.					
32483971	3	20	theme	daytime	299:305	arg1	productivity					312:323	the daytime work productivity	295:323	the daytime work productivity	295:323	RLS could severely impact the daytime work productivity and the life quality of patients.					
32483971	4	21	theme	accurate	418:425	arg1	diagnosis					438:446	an accurate and timely diagnosis	415:446	an accurate and timely diagnosis	415:446	However, the current diagnostic methods fail to provide an accurate and timely diagnosis, and the pathophysiology of RLS is not fully understood.					
32483971	8	22	theme	substantial	1042:1052	arg1	differences					1054:1064	substantial differences	1042:1064	substantial differences between RLS patients and controls	1042:1098	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	10	23	theme	RLS	1405:1407	arg1	cohorts					1409:1415	the RLS cohorts	1401:1415	the RLS cohorts	1401:1415	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	14	24	theme	targeted	2029:2036	arg1	interventions					2050:2062	novel targeted therapeutic interventions	2023:2062	novel targeted therapeutic interventions	2023:2062	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	8	25	dep	RLS	1074:1076	arg1	controls					1091:1098	controls	1091:1098	controls	1091:1098	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	8	25	dep	RLS	1074:1076	arg1	patients					1078:1085	patients	1078:1085	patients	1078:1085	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	3	26	theme	work	307:310	arg1	productivity					312:323	the daytime work productivity	295:323	the daytime work productivity	295:323	RLS could severely impact the daytime work productivity and the life quality of patients.					
32483971	5	27	theme	RLS	576:578	arg1	mechanisms					562:571	the underlying biochemical mechanisms	535:571	the underlying biochemical mechanisms of RLS	535:578	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	6	28	theme	glycomics	732:740	arg1	approach					742:749	a shotgun glycomics approach	722:749	a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients	722:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	14	29	theme	novel	2023:2027	arg1	interventions					2050:2062	novel targeted therapeutic interventions	2023:2062	novel targeted therapeutic interventions	2023:2062	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	5	30	theme	guiding	678:684	arg1	interventions					686:698	guiding interventions	678:698	guiding interventions	678:698	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	0	31	theme	Glycomics	6:14	arg1	Profiling					16:24	Serum Glycomics Profiling	0:24	Serum Glycomics Profiling of Patients with Primary Restless	0:58	Serum Glycomics Profiling of Patients with Primary Restless Legs Syndrome Using LC-MS/MS.					
32483971	4	32	theme	timely	431:436	arg1	diagnosis					438:446	an accurate and timely diagnosis	415:446	an accurate and timely diagnosis	415:446	However, the current diagnostic methods fail to provide an accurate and timely diagnosis, and the pathophysiology of RLS is not fully understood.					
32483971	6	33	theme	shotgun	724:730	arg1	approach					742:749	a shotgun glycomics approach	722:749	a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients	722:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	14	34	theme	molecular	1921:1929	arg1	mechanism					1931:1939	the molecular mechanism	1917:1939	the molecular mechanism of RLS	1917:1946	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	0	35	theme	Serum	0:4	arg1	Profiling					16:24	Serum Glycomics Profiling	0:24	Serum Glycomics Profiling of Patients with Primary Restless	0:58	Serum Glycomics Profiling of Patients with Primary Restless Legs Syndrome Using LC-MS/MS.					
32483971	10	36	theme	multiple	1475:1482	arg1	correction					1492:1501	the Benjamini-Hochberg multiple testing correction	1452:1501	the Benjamini-Hochberg multiple testing correction	1452:1501	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	9	37	theme	HexNAc6Hex6Fuc1NeuAc3	1237:1257	arg1	composition					1199:1209	the composition	1195:1209	the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2	1195:1284	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	1	38	theme	Restless	90:97	arg1	RLS					114:116	RLS	114:116	RLS	114:116	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	1	38	theme	Restless	90:97	arg1	sleep					161:165	sleep	161:165	sleep	161:165	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	1	38	theme	Restless	90:97	arg1	syndrome					104:111	Restless legs syndrome	90:111	Restless legs syndrome (RLS)	90:117	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	14	39	theme	alterations	1872:1882	arg1	discovery					1835:1843	The discovery	1831:1843	The discovery of the N-glycan expression alterations	1831:1882	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	14	39	theme	alterations	1872:1882	arg1	useful					1893:1898	useful	1893:1898	useful	1893:1898	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	13	40	with	individuals	1809:1819	arg1	RLS					1826:1828	RLS	1826:1828	RLS	1826:1828	These results demonstrate that it is possible to detect specific glycome traits in individuals with RLS.					
32483971	5	41	theme	powerful	634:641	arg1	biomarkers					643:652	powerful biomarkers	634:652	powerful biomarkers for early detection and guiding interventions	634:698	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	7	42	theme	isomeric	879:886	arg1	quantitations					888:900	isomeric quantitations	879:900	isomeric quantitations	879:900	Glycan profiles and isomeric quantitations were assessed by liquid chromatography-mass spectrometry analysis and compared with healthy controls.					
32483971	1	43	theme	legs	99:102	arg1	RLS					114:116	RLS	114:116	RLS	114:116	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	1	43	theme	legs	99:102	arg1	sleep					161:165	sleep	161:165	sleep	161:165	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	1	43	theme	legs	99:102	arg1	syndrome					104:111	Restless legs syndrome	90:111	Restless legs syndrome (RLS)	90:117	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	10	44	theme	significant	1422:1432	arg1	differences					1434:1444	significant differences	1422:1444	significant differences	1422:1444	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	10	45	from	structures	1387:1396	arg1	cohorts					1409:1415	the RLS cohorts	1401:1415	the RLS cohorts	1401:1415	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	3	46	theme	life	333:336	arg1	quality					338:344	the life quality	329:344	the life quality of patients	329:356	RLS could severely impact the daytime work productivity and the life quality of patients.					
32483971	6	47	theme	glycan	795:800	arg1	candidates					812:821	the potential glycan biomarker candidates	781:821	the potential glycan biomarker candidates in the blood serum of RLS patients	781:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	13	48	from	traits	1799:1804	arg1	individuals					1809:1819	individuals	1809:1819	individuals with RLS	1809:1828	These results demonstrate that it is possible to detect specific glycome traits in individuals with RLS.					
32483971	2	49	theme	general	249:255	arg1	population					257:266	the general population	245:266	the general population	245:266	The prevalence of RLS is at ∼5-15% in the general population.					
32483971	12	50	theme	p	1675:1675	arg1	HexNAc5Hex6NeuAc3					1647:1663	HexNAc5Hex6NeuAc3	1647:1663	HexNAc5Hex6NeuAc3 (5603-5) (p < 0.001)	1647:1684	HexNAc6Hex7NeuAc3 (6703-2) and HexNAc5Hex6NeuAc3 (5603-5) (p < 0.001) were expressed higher in the RLS cases.					
32483971	12	50	theme	p	1675:1675	arg1	<					1677:1677	p < 0.001	1675:1683	p < 0.001	1675:1683	HexNAc6Hex7NeuAc3 (6703-2) and HexNAc5Hex6NeuAc3 (5603-5) (p < 0.001) were expressed higher in the RLS cases.					
32483971	14	51	theme	expression	1861:1870	arg1	alterations					1872:1882	the N-glycan expression alterations	1848:1882	the N-glycan expression alterations	1848:1882	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	9	52	theme	HexNAc5Hex6Fuc1NeuAc2	1264:1284	arg1	composition					1199:1209	the composition	1195:1209	the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2	1195:1284	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	14	53	theme	diagnostic	1987:1996	arg1	methods					1998:2004	diagnostic methods	1987:2004	diagnostic methods	1987:2004	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	1	54	theme	neurological	171:182	arg1	disorder					197:204	a sleep and neurological sensorimotor disorder	159:204	disorder	197:204	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	0	55	theme	Patients	29:36	arg1	Profiling					16:24	Serum Glycomics Profiling	0:24	Serum Glycomics Profiling of Patients with Primary Restless	0:58	Serum Glycomics Profiling of Patients with Primary Restless Legs Syndrome Using LC-MS/MS.					
32483971	4	56	theme	RLS	476:478	arg1	pathophysiology					457:471	the pathophysiology	453:471	the pathophysiology of RLS	453:478	However, the current diagnostic methods fail to provide an accurate and timely diagnosis, and the pathophysiology of RLS is not fully understood.					
32483971	5	57	theme	early	658:662	arg1	detection					664:672	early detection	658:672	early detection	658:672	Glycomics can help to unravel the underlying biochemical mechanisms of RLS, to identify specific glycome changes, and to develop powerful biomarkers for early detection and guiding interventions.					
32483971	1	58	theme	sensorimotor	184:195	arg1	disorder					197:204	a sleep and neurological sensorimotor disorder	159:204	disorder	197:204	Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is a sleep and neurological sensorimotor disorder.					
32483971	3	59	theme	patients	349:356	arg1	quality					338:344	the life quality	329:344	the life quality of patients	329:356	RLS could severely impact the daytime work productivity and the life quality of patients.					
32483971	3	59	theme	patients	349:356	arg1	productivity					312:323	the daytime work productivity	295:323	the daytime work productivity	295:323	RLS could severely impact the daytime work productivity and the life quality of patients.					
32483971	10	60	theme	testing	1484:1490	arg1	correction					1492:1501	the Benjamini-Hochberg multiple testing correction	1452:1501	the Benjamini-Hochberg multiple testing correction	1452:1501	Furthermore, 23 isomeric structures in the RLS cohorts show significant differences after the Benjamini-Hochberg multiple testing correction.					
32483971	6	61	theme	biomarker	802:810	arg1	candidates					812:821	the potential glycan biomarker candidates	781:821	the potential glycan biomarker candidates in the blood serum of RLS patients	781:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	0	62	theme	Primary	43:49	arg1	Restless					51:58	Primary Restless	43:58	Primary Restless	43:58	Serum Glycomics Profiling of Patients with Primary Restless Legs Syndrome Using LC-MS/MS.					
32483971	4	63	theme	current	372:378	arg1	methods					391:397	the current diagnostic methods	368:397	the current diagnostic methods	368:397	However, the current diagnostic methods fail to provide an accurate and timely diagnosis, and the pathophysiology of RLS is not fully understood.					
32483971	9	64	theme	significant	1300:1310	arg1	alteration					1312:1321	the most significant alteration	1291:1321	the most significant alteration in the expression profile (p < 0.001)	1291:1359	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	6	65	theme	blood	830:834	arg1	serum					836:840	the blood serum	826:840	the blood serum of RLS patients	826:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	2	66	from	%	240:240	arg1	population					257:266	the general population	245:266	the general population	245:266	The prevalence of RLS is at ∼5-15% in the general population.					
32483971	8	67	theme	multiple	1129:1136	arg1	correction					1146:1155	the Benjamini-Hochberg multiple testing correction	1106:1155	the Benjamini-Hochberg multiple testing correction	1106:1155	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	6	68	theme	potential	785:793	arg1	candidates					812:821	the potential glycan biomarker candidates	781:821	the potential glycan biomarker candidates in the blood serum of RLS patients	781:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	7	69	theme	chromatography-mass	926:944	arg1	analysis					959:966	liquid chromatography-mass spectrometry analysis	919:966	liquid chromatography-mass spectrometry analysis	919:966	Glycan profiles and isomeric quantitations were assessed by liquid chromatography-mass spectrometry analysis and compared with healthy controls.					
32483971	9	70	theme	HexNAc6Hex8Fuc1NeuAc2	1214:1234	arg1	composition					1199:1209	the composition	1195:1209	the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2	1195:1284	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	12	71	theme	RLS	1715:1717	arg1	cases					1719:1723	the RLS cases	1711:1723	the RLS cases	1711:1723	HexNAc6Hex7NeuAc3 (6703-2) and HexNAc5Hex6NeuAc3 (5603-5) (p < 0.001) were expressed higher in the RLS cases.					
32483971	7	72	theme	healthy	986:992	arg1	controls					994:1001	healthy controls	986:1001	healthy controls	986:1001	Glycan profiles and isomeric quantitations were assessed by liquid chromatography-mass spectrometry analysis and compared with healthy controls.					
32483971	14	73	dep	refined	1965:1971	arg1	methods					1998:2004	diagnostic methods	1987:2004	diagnostic methods	1987:2004	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	14	74	theme	N-glycan	1852:1859	arg1	alterations					1872:1882	the N-glycan expression alterations	1848:1882	the N-glycan expression alterations	1848:1882	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	9	75	with	glycans	1182:1188	arg1	composition					1199:1209	the composition	1195:1209	the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2	1195:1284	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	9	76	from	alteration	1312:1321	arg1	profile					1341:1347	the expression profile	1326:1347	the expression profile (p < 0.001)	1326:1359	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	9	76	from	alteration	1312:1321	arg1	<					1352:1352	p < 0.001	1350:1358	p < 0.001	1350:1358	Among those structures, glycans with the composition of HexNAc6Hex8Fuc1NeuAc2, HexNAc6Hex6Fuc1NeuAc3, and HexNAc5Hex6Fuc1NeuAc2 show the most significant alteration in the expression profile (p < 0.001).					
32483971	4	77	theme	diagnostic	380:389	arg1	methods					391:397	the current diagnostic methods	368:397	the current diagnostic methods	368:397	However, the current diagnostic methods fail to provide an accurate and timely diagnosis, and the pathophysiology of RLS is not fully understood.					
32483971	2	78	theme	RLS	225:227	arg1	prevalence					211:220	The prevalence	207:220	The prevalence of RLS	207:227	The prevalence of RLS is at ∼5-15% in the general population.					
32483971	11	79	theme	p	1567:1567	arg1	HexNAc6Hex7NeuAc3					1539:1555	HexNAc6Hex7NeuAc3	1539:1555	HexNAc6Hex7NeuAc3 (6703-1) (p < 0.001)	1539:1576	HexNAc4Hex5Fuc1NeuAc2 (4512-3) and HexNAc6Hex7NeuAc3 (6703-1) (p < 0.001) were downexpressed in the RLS cohort.					
32483971	11	79	theme	p	1567:1567	arg1	<					1569:1569	p < 0.001	1567:1575	p < 0.001	1567:1575	HexNAc4Hex5Fuc1NeuAc2 (4512-3) and HexNAc6Hex7NeuAc3 (6703-1) (p < 0.001) were downexpressed in the RLS cohort.					
32483971	8	80	theme	testing	1138:1144	arg1	correction					1146:1155	the Benjamini-Hochberg multiple testing correction	1106:1155	the Benjamini-Hochberg multiple testing correction	1106:1155	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
32483971	14	81	theme	RLS	1944:1946	arg1	mechanism					1931:1939	the molecular mechanism	1917:1939	the molecular mechanism of RLS	1917:1946	The discovery of the N-glycan expression alterations might be useful in understanding the molecular mechanism of RLS, developing more refined and objective diagnostic methods, and discovering novel targeted therapeutic interventions.					
32483971	7	82	theme	spectrometry	946:957	arg1	analysis					959:966	liquid chromatography-mass spectrometry analysis	919:966	liquid chromatography-mass spectrometry analysis	919:966	Glycan profiles and isomeric quantitations were assessed by liquid chromatography-mass spectrometry analysis and compared with healthy controls.					
32483971	6	83	from	candidates	812:821	arg1	serum					836:840	the blood serum	826:840	the blood serum of RLS patients	826:856	Herein, we undertook a shotgun glycomics approach to determine and characterize the potential glycan biomarker candidates in the blood serum of RLS patients.					
32483971	8	84	theme	Benjamini-Hochberg	1110:1127	arg1	correction					1146:1155	the Benjamini-Hochberg multiple testing correction	1106:1155	the Benjamini-Hochberg multiple testing correction	1106:1155	24 N-glycan biomarker candidates show substantial differences between RLS patients and controls after the Benjamini-Hochberg multiple testing correction.					
33880112	4	0	theme	precision	943:951	arg1	imaging					953:959	the precision imaging	939:959	the precision imaging of ASCVD	939:968	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	5	1	from	accumulation	1159:1170	arg1	AP					1179:1180	the AP	1175:1180	the AP	1175:1180	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	4	2	theme	Gd-based	841:848	arg1	CAs					867:869	CAs	867:869	CAs	867:869	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	4	2	theme	Gd-based	841:848	arg1	agents					859:864	Gd-based contrast agents	841:864	Gd-based contrast agents (CAs)	841:870	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	8	3	theme	probes	1767:1772	arg1	interaction					1746:1756	interaction	1746:1756	interaction of these probes with the AP	1746:1784	Results demonstrate that the Hydrodenticity of cHANPs and Ab36-cHANPs is preserved in this complex system and, preliminarily, that interaction of these probes with the AP is present.					
33880112	1	4	theme	worldwide	163:171	arg1	cause					148:152	the main cause	139:152	the main cause of death worldwide	139:171	Despite the progress in cardiovascular research, atherosclerosis still represents the main cause of death worldwide.					
33880112	4	5	theme	class	1004:1008	arg1	performance					984:994	the performance	980:994	the performance of this class of CAs	980:1015	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	5	6	with	targeting	1238:1246	arg1	effect					1275:1280	the overall effect	1263:1280	the overall effect of allowing an earlier and more accurate diagnosis	1263:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	7	7	theme	cHANPs	1497:1502	arg1	surface					1486:1492	the surface	1482:1492	the surface of cHANPs	1482:1502	In addition, the surface of cHANPs was decorated with the antibody anti-CD36 (Ab36-cHANPs) for the active targeting of AP-associated macrophages.					
33880112	8	8	theme	Ab36-cHANPs	1673:1683	arg1	Hydrodenticity					1644:1657	the Hydrodenticity	1640:1657	the Hydrodenticity of cHANPs and Ab36-cHANPs	1640:1683	Results demonstrate that the Hydrodenticity of cHANPs and Ab36-cHANPs is preserved in this complex system and, preliminarily, that interaction of these probes with the AP is present.					
33880112	4	9	theme	agents	859:864	arg1	properties					827:836	relaxometric properties	814:836	relaxometric properties of Gd-based contrast agents (CAs)	814:870	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	2	10	theme	computed	314:321	arg1	CT					335:336	CT	335:336	CT	335:336	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	10	theme	computed	314:321	arg1	tomography					323:332	computed tomography	314:332	computed tomography (CT)	314:337	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	1	11	from	progress	69:76	arg1	research					96:103	cardiovascular research	81:103	cardiovascular research	81:103	Despite the progress in cardiovascular research, atherosclerosis still represents the main cause of death worldwide.					
33880112	5	12	theme	nanoparticle	1185:1196	arg1	decoration					1206:1215	nanoparticle surface decoration	1185:1215	nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis	1185:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	4	13	theme	contrast	850:857	arg1	CAs					867:869	CAs	867:869	CAs	867:869	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	4	13	theme	contrast	850:857	arg1	agents					859:864	Gd-based contrast agents	841:864	Gd-based contrast agents (CAs)	841:870	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	6	14	link	crosslinked	1358:1368	arg1	Nanoparticles					1386:1398	crosslinked Hyaluronic Acid Nanoparticles	1358:1398	crosslinked Hyaluronic Acid Nanoparticles (cHANPs)	1358:1407	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	6	14	link	crosslinked	1358:1368	arg1	cHANPs					1401:1406	cHANPs	1401:1406	cHANPs	1401:1406	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	5	15	theme	surface	1198:1204	arg1	decoration					1206:1215	nanoparticle surface decoration	1185:1215	nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis	1185:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	4	16	theme	valuable	917:924	arg1	tool					926:929	a valuable tool	915:929	a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity	915:1048	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	7	17	theme	AP-associated	1588:1600	arg1	macrophages					1602:1612	AP-associated macrophages	1588:1612	AP-associated macrophages	1588:1612	In addition, the surface of cHANPs was decorated with the antibody anti-CD36 (Ab36-cHANPs) for the active targeting of AP-associated macrophages.					
33880112	6	18	theme	plaque	1461:1466	arg1	environment					1424:1434	the complex environment	1412:1434	the complex environment of human atherosclerotic plaque	1412:1466	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	2	19	theme	high	403:406	arg1	cause					441:445	the main cause	432:445	the main cause of severe clinical events as stroke and heart attack	432:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	19	theme	high	403:406	arg1	risk					408:411	high risk	403:411	high risk of plaque rupture	403:429	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	6	20	theme	Hyaluronic	1370:1379	arg1	Acid					1381:1384	Hyaluronic Acid	1370:1384	crosslinked Hyaluronic Acid Nanoparticles (cHANPs)	1358:1407	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	2	21	theme	events	466:471	arg1	cause					441:445	the main cause	432:445	the main cause of severe clinical events as stroke and heart attack	432:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	21	theme	events	466:471	arg1	risk					408:411	high risk	403:411	high risk of plaque rupture	403:429	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	1	22	theme	cardiovascular	81:94	arg1	research					96:103	cardiovascular research	81:103	cardiovascular research	81:103	Despite the progress in cardiovascular research, atherosclerosis still represents the main cause of death worldwide.					
33880112	5	23	theme	accurate	1314:1321	arg1	diagnosis					1323:1331	an earlier and more accurate diagnosis	1294:1331	an earlier and more accurate diagnosis	1294:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	8	24	theme	cHANPs	1662:1667	arg1	Hydrodenticity					1644:1657	the Hydrodenticity	1640:1657	the Hydrodenticity of cHANPs and Ab36-cHANPs	1640:1683	Results demonstrate that the Hydrodenticity of cHANPs and Ab36-cHANPs is preserved in this complex system and, preliminarily, that interaction of these probes with the AP is present.					
33880112	8	25	with	interaction	1746:1756	arg1	AP					1783:1784	the AP	1779:1784	the AP	1779:1784	Results demonstrate that the Hydrodenticity of cHANPs and Ab36-cHANPs is preserved in this complex system and, preliminarily, that interaction of these probes with the AP is present.					
33880112	5	26	theme	hydrogel	1066:1073	arg1	nanoparticles					1075:1087	hydrogel nanoparticles	1066:1087	hydrogel nanoparticles encapsulating Gd-DTPA	1066:1109	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	5	27	theme	CA-specific	1147:1157	arg1	accumulation					1159:1170	providing CA-specific accumulation	1137:1170	providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis	1137:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	2	28	theme	patients	391:398	arg1	identification					373:386	the identification	369:386	the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack	369:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	29	theme	main	436:439	arg1	cause					441:445	the main cause	432:445	the main cause of severe clinical events as stroke and heart attack	432:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	29	theme	main	436:439	arg1	risk					408:411	high risk	403:411	high risk of plaque rupture	403:429	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	3	30	theme	plaques	726:732	arg1	identification					695:708	the identification	691:708	the identification of "vulnerable" plaques	691:732	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	4	31	theme	relaxometric	814:825	arg1	properties					827:836	relaxometric properties	814:836	relaxometric properties of Gd-based contrast agents (CAs)	814:870	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	2	32	from	identification	373:386	arg1	cause					441:445	the main cause	432:445	the main cause of severe clinical events as stroke and heart attack	432:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	32	from	identification	373:386	arg1	risk					408:411	high risk	403:411	high risk of plaque rupture	403:429	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	3	33	theme	high	560:563	arg1	resolution					573:582	very high spatial resolution	555:582	very high spatial resolution	555:582	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	8	34	dep	preserved	1688:1696	arg1	present					1789:1795	present	1789:1795	present	1789:1795	Results demonstrate that the Hydrodenticity of cHANPs and Ab36-cHANPs is preserved in this complex system and, preliminarily, that interaction of these probes with the AP is present.					
33880112	7	35	theme	macrophages	1602:1612	arg1	targeting					1575:1583	the active targeting	1564:1583	the active targeting of AP-associated macrophages	1564:1612	In addition, the surface of cHANPs was decorated with the antibody anti-CD36 (Ab36-cHANPs) for the active targeting of AP-associated macrophages.					
33880112	8	36	theme	complex	1706:1712	arg1	system					1714:1719	this complex system	1701:1719	this complex system	1701:1719	Results demonstrate that the Hydrodenticity of cHANPs and Ab36-cHANPs is preserved in this complex system and, preliminarily, that interaction of these probes with the AP is present.					
33880112	2	37	theme	rupture	423:429	arg1	cause					441:445	the main cause	432:445	the main cause of severe clinical events as stroke and heart attack	432:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	37	theme	rupture	423:429	arg1	risk					408:411	high risk	403:411	high risk of plaque rupture	403:429	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	3	38	theme	vulnerable	714:723	arg1	plaques					726:732	"vulnerable" plaques	713:732	"vulnerable" plaques	713:732	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	3	39	theme	plaque	644:649	arg1	characterization					608:623	a better characterization	599:623	a better characterization of atherosclerotic plaque (AP) anatomy and composition	599:678	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	5	40	theme	AP	1255:1256	arg1	targeting					1238:1246	an active targeting	1228:1246	an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis	1228:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	2	41	theme	plaque	416:421	arg1	rupture					423:429	plaque rupture	416:429	plaque rupture	416:429	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	0	42	theme	Atherosclerosis	40:54	arg1	Imaging					29:35	Imaging	29:35	Imaging of Atherosclerosis	29:54	Targeting Nanostrategies for Imaging of Atherosclerosis.					
33880112	3	43	theme	"	724:724	arg1	plaques					726:732	"vulnerable" plaques	713:732	"vulnerable" plaques	713:732	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	4	44	theme	Hydrodenticity	889:902	arg1	effect					879:884	the effect	875:884	the effect of Hydrodenticity	875:902	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	5	45	theme	earlier	1297:1303	arg1	diagnosis					1323:1331	an earlier and more accurate diagnosis	1294:1331	an earlier and more accurate diagnosis	1294:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	6	46	theme	crosslinked	1358:1368	arg1	Nanoparticles					1386:1398	crosslinked Hyaluronic Acid Nanoparticles	1358:1398	crosslinked Hyaluronic Acid Nanoparticles (cHANPs)	1358:1407	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	6	46	theme	crosslinked	1358:1368	arg1	cHANPs					1401:1406	cHANPs	1401:1406	cHANPs	1401:1406	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	2	47	theme	clinical	457:464	arg1	events					466:471	severe clinical events	450:471	severe clinical events as stroke and heart attack	450:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	7	48	theme	active	1568:1573	arg1	targeting					1575:1583	the active targeting	1564:1583	the active targeting of AP-associated macrophages	1564:1612	In addition, the surface of cHANPs was decorated with the antibody anti-CD36 (Ab36-cHANPs) for the active targeting of AP-associated macrophages.					
33880112	2	49	theme	severe	450:455	arg1	events					466:471	severe clinical events	450:471	severe clinical events as stroke and heart attack	450:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	3	50	theme	atherosclerotic	628:642	arg1	plaque					644:649	atherosclerotic plaque	628:649	atherosclerotic plaque (AP) anatomy and composition	628:678	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	3	50	theme	atherosclerotic	628:642	arg1	AP					652:653	AP	652:653	AP	652:653	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	3	51	theme	spatial	565:571	arg1	resolution					573:582	very high spatial resolution	555:582	very high spatial resolution	555:582	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	4	52	theme	ASCVD	964:968	arg1	imaging					953:959	the precision imaging	939:959	the precision imaging of ASCVD	939:968	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	2	53	theme	Disease	234:240	arg1	diagnosis					190:198	the diagnosis	186:198	the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD)	186:248	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	6	54	theme	complex	1416:1422	arg1	environment					1424:1434	the complex environment	1412:1434	the complex environment of human atherosclerotic plaque	1412:1466	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	4	55	theme	systemic	1032:1039	arg1	toxicity					1041:1048	systemic toxicity	1032:1048	systemic toxicity	1032:1048	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	5	56	theme	providing	1137:1145	arg1	accumulation					1159:1170	providing CA-specific accumulation	1137:1170	providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis	1137:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	2	57	theme	Cardiovascular	219:232	arg1	ASCVD					243:247	ASCVD	243:247	ASCVD	243:247	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	57	theme	Cardiovascular	219:232	arg1	Disease					234:240	Atherosclerotic Cardiovascular Disease	203:240	Atherosclerotic Cardiovascular Disease (ASCVD)	203:248	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	3	58	theme	better	601:606	arg1	characterization					608:623	a better characterization	599:623	a better characterization of atherosclerotic plaque (AP) anatomy and composition	599:678	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	1	59	theme	main	143:146	arg1	cause					148:152	the main cause	139:152	the main cause of death worldwide	139:171	Despite the progress in cardiovascular research, atherosclerosis still represents the main cause of death worldwide.					
33880112	2	60	theme	X-ray	292:296	arg1	angiography					298:308	X-ray angiography	292:308	X-ray angiography	292:308	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	61	theme	Atherosclerotic	203:217	arg1	ASCVD					243:247	ASCVD	243:247	ASCVD	243:247	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	61	theme	Atherosclerotic	203:217	arg1	Disease					234:240	Atherosclerotic Cardiovascular Disease	203:240	Atherosclerotic Cardiovascular Disease (ASCVD)	203:248	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	4	62	theme	hydrogel	752:759	arg1	matrices					761:768	hydrogel matrices	752:768	hydrogel matrices	752:768	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	3	63	theme	resonance	510:518	arg1	imaging					520:526	Magnetic resonance imaging	501:526	Magnetic resonance imaging	501:526	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	5	64	theme	overall	1267:1273	arg1	effect					1275:1280	the overall effect	1263:1280	the overall effect of allowing an earlier and more accurate diagnosis	1263:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33880112	2	65	theme	heart	487:491	arg1	attack					493:498	heart attack	487:498	heart attack	487:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	4	66	theme	CAs	1013:1015	arg1	class					1004:1008	this class	999:1008	this class of CAs	999:1015	In this context, hydrogel matrices, which have been demonstrated able to boost relaxometric properties of Gd-based contrast agents (CAs) by the effect of Hydrodenticity, represent a valuable tool towards the precision imaging of ASCVD improving the performance of this class of CAs while reducing systemic toxicity.					
33880112	2	67	theme	imaging	260:266	arg1	methodologies					268:280	imaging methodologies	260:280	imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack	260:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	67	theme	imaging	260:266	arg1	angiography					298:308	X-ray angiography	292:308	X-ray angiography	292:308	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	67	theme	imaging	260:266	arg1	tomography					323:332	computed tomography	314:332	computed tomography (CT)	314:337	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	68	from	risk	408:411	arg1	patients					391:398	patients	391:398	patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack	391:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	2	68	from	risk	408:411	arg1	identification					373:386	the identification	369:386	the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack	369:498	Clinically, the diagnosis of Atherosclerotic Cardiovascular Disease (ASCVD) relies on imaging methodologies including X-ray angiography and computed tomography (CT), which however still fails in the identification of patients at high risk of plaque rupture, the main cause of severe clinical events as stroke and heart attack.					
33880112	3	69	theme	Magnetic	501:508	arg1	imaging					520:526	Magnetic resonance imaging	501:526	Magnetic resonance imaging	501:526	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	6	70	theme	atherosclerotic	1445:1459	arg1	plaque					1461:1466	human atherosclerotic plaque	1439:1466	human atherosclerotic plaque	1439:1466	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	3	71	dep	plaque	644:649	arg1	composition					668:678	composition	668:678	composition	668:678	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	3	71	dep	plaque	644:649	arg1	anatomy					656:662	anatomy	656:662	anatomy	656:662	Magnetic resonance imaging, which is characterized by very high spatial resolution, could provide a better characterization of atherosclerotic plaque (AP) anatomy and composition, aiding in the identification of "vulnerable" plaques.					
33880112	6	72	theme	Acid	1381:1384	arg1	Nanoparticles					1386:1398	crosslinked Hyaluronic Acid Nanoparticles	1358:1398	crosslinked Hyaluronic Acid Nanoparticles (cHANPs)	1358:1407	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	6	72	theme	Acid	1381:1384	arg1	cHANPs					1401:1406	cHANPs	1401:1406	cHANPs	1401:1406	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	1	73	theme	death	157:161	arg1	worldwide					163:171	death worldwide	157:171	death worldwide	157:171	Despite the progress in cardiovascular research, atherosclerosis still represents the main cause of death worldwide.					
33880112	6	74	theme	human	1439:1443	arg1	plaque					1461:1466	human atherosclerotic plaque	1439:1466	human atherosclerotic plaque	1439:1466	In this work, we tested crosslinked Hyaluronic Acid Nanoparticles (cHANPs) in the complex environment of human atherosclerotic plaque.					
33880112	5	75	theme	active	1231:1236	arg1	targeting					1238:1246	an active targeting	1228:1246	an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis	1228:1331	In particular, hydrogel nanoparticles encapsulating Gd-DTPA can further contribute to providing CA-specific accumulation in the AP by nanoparticle surface decoration triggering an active targeting of the AP with the overall effect of allowing an earlier and more accurate diagnosis.					
33142661	6	0	theme	paper	1047:1051	arg1	density					1053:1059	a lower paper density	1039:1059	a lower paper density	1039:1059	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	7	1	theme	BC-org	1269:1274	arg1	nanopapers					1276:1285	BC-org nanopapers	1269:1285	BC-org nanopapers	1269:1285	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	2	2	from	Pollutants	290:299	arg1	nanoscale					308:316	nanoscale	308:316	nanoscale	308:316	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	1	3	theme	fresh	248:252	arg1	sources					260:266	fresh water sources	248:266	fresh water sources	248:266	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	5	4	theme	papermaking	921:931	arg1	process					933:939	a simple papermaking process	912:939	a simple papermaking process	912:939	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	7	5	contain	have	1293:1296	arg2	sizes					1303:1307	pore sizes	1298:1307	pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions	1298:1374	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	7	5	contain	have	1293:1296	arg1	nanopapers					1276:1285	BC-org nanopapers	1269:1285	BC-org nanopapers	1269:1285	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	6	6	theme	aqueous	1137:1143	arg1	dispersions					1145:1155	aqueous dispersions	1137:1155	aqueous dispersions	1137:1155	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	1	7	theme	water	254:258	arg1	sources					260:266	fresh water sources	248:266	fresh water sources	248:266	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	0	8	theme	pollutants	100:109	arg1	rejection					78:86	rejection	78:86	rejection	78:86	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	0	8	theme	pollutants	100:109	arg1	permeance					64:72	permeance	64:72	permeance	64:72	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	6	9	theme	cellulose	1207:1215	arg1	membranes					1227:1235	bacterial cellulose nanopaper membranes	1197:1235	bacterial cellulose nanopaper membranes	1197:1235	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	6	10	theme	lower	1041:1045	arg1	density					1053:1059	a lower paper density	1039:1059	a lower paper density	1039:1059	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	0	11	theme	nm-sized	91:98	arg1	pollutants					100:109	nm-sized pollutants	91:109	nm-sized pollutants	91:109	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	7	12	theme	higher	1252:1257	arg1	porosity					1259:1266	their higher porosity	1246:1266	their higher porosity	1246:1266	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	6	13	theme	bacterial	1197:1205	arg1	membranes					1227:1235	bacterial cellulose nanopaper membranes	1197:1235	bacterial cellulose nanopaper membranes	1197:1235	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	5	14	theme	alcohol	880:886	arg1	liquids					871:877	organic liquids	863:877	organic liquids (alcohol, ketone, ether)	863:902	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	14	theme	alcohol	880:886	arg1	ether					897:901	alcohol, ketone, ether	880:901	ether	897:901	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	15	theme	organic	863:869	arg1	liquids					871:877	organic liquids	863:877	organic liquids (alcohol, ketone, ether)	863:902	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	15	theme	organic	863:869	arg1	ether					897:901	alcohol, ketone, ether	880:901	ether	897:901	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	7	16	dep	contaminants	1402:1413	arg1	size					1419:1422	the size	1415:1422	contaminants the size of viruses	1402:1433	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	4	17	theme	nanopaper	676:684	arg1	filters					686:692	so far nanopaper filters	669:692	so far nanopaper filters	669:692	Unfortunately, so far nanopaper filters suffer from limited permeance and thus efficiency.					
33142661	7	18	from	permeance	1473:1481	arg1	mechanism					1455:1463	a size-exclusion mechanism	1438:1463	a size-exclusion mechanism at high permeance	1438:1481	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	7	19	theme	high	1468:1471	arg1	permeance					1473:1481	high permeance	1468:1481	high permeance	1468:1481	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	6	20	theme	higher	1011:1016	arg1	increased					1067:1075	increased	1067:1075	increased	1067:1075	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	6	20	theme	higher	1011:1016	arg1	permeance					1018:1026	40 times higher permeance	1002:1026	40 times higher permeance	1002:1026	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	7	21	theme	BC	1332:1333	arg1	nanopapers					1335:1344	BC nanopapers	1332:1344	BC nanopapers made from aqueous dispersions	1332:1374	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	7	22	theme	size-exclusion	1440:1453	arg1	mechanism					1455:1463	a size-exclusion mechanism	1438:1463	a size-exclusion mechanism at high permeance	1438:1481	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	7	23	theme	aqueous	1356:1362	arg1	dispersions					1364:1374	aqueous dispersions	1356:1374	aqueous dispersions	1356:1374	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	0	24	theme	nanocellulose	10:22	arg1	papers					24:29	Bacterial nanocellulose papers	0:29	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.	0:110	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	6	25	dep	caused	1029:1034	arg1	increased					1067:1075	increased	1067:1075	increased	1067:1075	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	6	25	dep	caused	1029:1034	arg1	permeance					1018:1026	40 times higher permeance	1002:1026	40 times higher permeance	1002:1026	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	7	26	theme	nm	1318:1319	arg1	sizes					1303:1307	pore sizes	1298:1307	pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions	1298:1374	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	7	27	theme	viruses	1427:1433	arg1	size					1419:1422	the size	1415:1422	contaminants the size of viruses	1402:1433	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	1	28	theme	whole	182:186	arg1	humankind					188:196	whole humankind	182:196	whole humankind	182:196	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	0	29	theme	Bacterial	0:8	arg1	papers					24:29	Bacterial nanocellulose papers	0:29	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.	0:110	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	5	30	dep	water	815:819	arg1	liquids					871:877	organic liquids	863:877	organic liquids (alcohol, ketone, ether)	863:902	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	30	dep	water	815:819	arg1	ether					897:901	alcohol, ketone, ether	880:901	ether	897:901	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	31	theme	low	843:845	arg1	tension					855:861	low surface tension	843:861	low surface tension	843:861	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	1	32	theme	important	274:282	arg1	task					284:287	an important task	271:287	an important task	271:287	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	1	32	theme	important	274:282	arg1	treatment					210:218	water treatment	204:218	water treatment to remediate wastewater and fresh water sources	204:266	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	5	33	dep	ether	897:901	arg1	ketone					889:894	alcohol, ketone, ether	880:901	ketone	889:894	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	3	34	from	alternatives	604:615	arg1	domain					646:651	this domain	641:651	this domain	641:651	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	3	35	theme	Cellulose	467:475	arg1	nanopapers					477:486	Cellulose nanopapers	467:486	Cellulose nanopapers	467:486	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	5	36	theme	surface	847:853	arg1	tension					855:861	low surface tension	843:861	low surface tension	843:861	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	2	37	theme	filtration	396:405	arg1	nanofiltration					432:445	nanofiltration	432:445	nanofiltration	432:445	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	2	37	theme	filtration	396:405	arg1	ultrafiltration					450:464	ultrafiltration	450:464	ultrafiltration	450:464	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	2	37	theme	filtration	396:405	arg1	processes					407:415	membrane filtration processes	387:415	membrane filtration processes	387:415	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	3	38	theme	renewable	503:511	arg1	resources					513:521	renewable resources	503:521	renewable resources	503:521	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	1	39	theme	water	204:208	arg1	task					284:287	an important task	271:287	an important task	271:287	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	1	39	theme	water	204:208	arg1	treatment					210:218	water treatment	204:218	water treatment to remediate wastewater and fresh water sources	204:266	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	7	40	theme	contaminants	1402:1413	arg1	removal					1391:1397	removal	1391:1397	removal of contaminants the size of viruses	1391:1433	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	2	41	theme	membrane	387:394	arg1	nanofiltration					432:445	nanofiltration	432:445	nanofiltration	432:445	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	2	41	theme	membrane	387:394	arg1	ultrafiltration					450:464	ultrafiltration	450:464	ultrafiltration	450:464	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	2	41	theme	membrane	387:394	arg1	processes					407:415	membrane filtration processes	387:415	membrane filtration processes	387:415	Pollutants in the nanoscale, such as viruses and macromolecules, are usually removed by means of membrane filtration processes, predominantly nanofiltration or ultrafiltration.					
33142661	0	42	theme	high	36:39	arg1	porosity					41:48	high porosity	36:48	high porosity	36:48	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	6	43	theme	organic	967:973	arg1	BC-org					984:989	BC-org	984:989	BC-org	984:989	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	6	43	theme	organic	967:973	arg1	liquids					975:981	organic liquids	967:981	organic liquids (BC-org)	967:990	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	3	44	theme	polymer	620:626	arg1	membranes					628:636	polymer membranes	620:636	polymer membranes	620:636	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	7	45	theme	similar	1321:1327	arg1	sizes					1303:1307	pore sizes	1298:1307	pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions	1298:1374	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	0	46	theme	optimized	54:62	arg1	permeance					64:72	permeance	64:72	permeance	64:72	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	3	47	theme	versatile	594:602	arg1	alternatives					604:615	versatile alternatives	594:615	versatile alternatives to polymer membranes	594:636	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	0	48	with	papers	24:29	arg1	porosity					41:48	high porosity	36:48	high porosity	36:48	Bacterial nanocellulose papers with high porosity for optimized permeance and rejection of nm-sized pollutants.					
33142661	5	49	theme	simple	914:919	arg1	process					933:939	a simple papermaking process	912:939	a simple papermaking process	912:939	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	50	theme	different	824:832	arg1	types					834:838	different types	824:838	different types of low surface tension	824:861	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	1	51	theme	clean	122:126	arg1	water					136:140	clean potable water	122:140	clean potable water	122:140	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	6	52	theme	nanopaper	1217:1225	arg1	membranes					1227:1235	bacterial cellulose nanopaper membranes	1197:1235	bacterial cellulose nanopaper membranes	1197:1235	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	1	53	theme	potable	128:134	arg1	water					136:140	clean potable water	122:140	clean potable water	122:140	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	1	54	theme	remediate	223:231	arg1	wastewater					233:242	remediate wastewater	223:242	remediate wastewater	223:242	Access to clean potable water is increasingly becoming a struggle for whole humankind, thus water treatment to remediate wastewater and fresh water sources is an important task.					
33142661	6	55	theme	membranes	1227:1235	arg1	efficiency					1183:1192	the efficiency	1179:1192	the efficiency of bacterial cellulose nanopaper membranes	1179:1235	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
33142661	5	56	theme	tension	855:861	arg1	water					815:819	water	815:819	water	815:819	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	5	56	theme	tension	855:861	arg1	types					834:838	different types	824:838	different types of low surface tension	824:861	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	4	57	theme	limited	706:712	arg1	permeance					714:722	limited permeance	706:722	limited permeance	706:722	Unfortunately, so far nanopaper filters suffer from limited permeance and thus efficiency.					
33142661	5	58	theme	bacterial	782:790	arg1	cellulose					792:800	bacterial cellulose	782:800	bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process	782:939	We here present nanopapers made from bacterial cellulose dispersed in water or different types of low surface tension organic liquids (alcohol, ketone, ether) through a simple papermaking process.					
33142661	3	59	dep	nanopapers	477:486	arg1	manufactured					527:538	manufactured	527:538	manufactured by papermaking	527:553	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	3	59	dep	nanopapers	477:486	arg1	prepared					489:496	prepared	489:496	prepared from renewable resources	489:521	Cellulose nanopapers, prepared from renewable resources and manufactured by papermaking, have recently been demonstrated to be versatile alternatives to polymer membranes in this domain.					
33142661	7	60	theme	pore	1298:1301	arg1	sizes					1303:1307	pore sizes	1298:1307	pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions	1298:1374	Despite their higher porosity, BC-org nanopapers still have pore sizes of 15-20 nm similar to BC nanopapers made from aqueous dispersions, thus enabling removal of contaminants the size of viruses by a size-exclusion mechanism at high permeance.					
33142661	6	61	theme	conventional	1099:1110	arg1	nanopapers					1112:1121	conventional nanopapers	1099:1121	conventional nanopapers produced from aqueous dispersions	1099:1155	Nanopapers prepared from organic liquids (BC-org) exhibited 40 times higher permeance, caused by a lower paper density hence increased porosity, compared to conventional nanopapers produced from aqueous dispersions, ultimately enhancing the efficiency of bacterial cellulose nanopaper membranes.					
34980348	3	0	theme	FTIR	659:662	arg1	spectra					664:670	FTIR spectra	659:670	FTIR spectra	659:670	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	5	1	theme	environmental	1281:1293	arg1	conditions					1295:1304	environmental conditions	1281:1304	environmental conditions	1281:1304	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	4	2	theme	TPS-PLA	975:981	arg1	surface					983:989	TPS-PLA surface	975:989	TPS-PLA surface	975:989	SEM micrographs highlighted consumption of TPS-PLA surface, while GPC analysis showed a decrease in PLA concentration by 20% during contact by UL.					
34980348	3	3	theme	thermal	757:763	arg1	stability					765:773	TPS thermal stability	753:773	TPS thermal stability	753:773	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	2	4	theme	composting	458:467	arg1	conditions					469:478	composting conditions	458:478	composting conditions	458:478	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	0	5	theme	mineralization	163:176	arg1	process					178:184	a fast mineralization process	156:184	a fast mineralization process	156:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	5	6	theme	end-life	1243:1250	arg1	management					1252:1261	end-life management	1243:1261	end-life management of bioplastics	1243:1276	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	4	7	theme	SEM	932:934	arg1	micrographs					936:946	SEM micrographs	932:946	SEM micrographs	932:946	SEM micrographs highlighted consumption of TPS-PLA surface, while GPC analysis showed a decrease in PLA concentration by 20% during contact by UL.					
34980348	3	8	theme	faecal	792:797	arg1	residues					799:806	faecal residues	792:806	faecal residues	792:806	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	1	9	theme	Tenebrionidae	259:271	arg1	family					285:290	Tenebrionidae Coleopteran family	259:290	Tenebrionidae Coleopteran family	259:290	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	2	10	used	used	303:306	arg2	They					293:296	They	293:296	They	293:296	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	1	11	theme	Coleopteran	273:283	arg1	family					285:290	Tenebrionidae Coleopteran family	259:290	Tenebrionidae Coleopteran family	259:290	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	3	12	theme	temperature	839:849	arg1	Tg					851:852	two glass transition temperature Tg	818:852	two glass transition temperature Tg	818:852	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	13	theme	TPS	753:755	arg1	stability					765:773	TPS thermal stability	753:773	TPS thermal stability	753:773	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	0	14	theme	novel	108:112	arg1	approach					143:150	a novel, challenging and sustainable approach	106:150	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	3	15	theme	film	510:513	arg1	contact					491:497	the contact	487:497	the contact of TPS-PLA film with UL for five days	487:535	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	2	16	theme	-poly	396:400	arg1	films					421:425	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	5	17	theme	TPS	1131:1133	arg1	biodigestion-biodegradation					1135:1161	TPS biodigestion-biodegradation	1131:1161	TPS biodigestion-biodegradation (80%)	1131:1167	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	5	17	theme	TPS	1131:1133	arg1	%					1166:1166	80%	1164:1166	80%	1164:1166	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	3	18	theme	PLA	745:747	arg1	stability					765:773	TPS thermal stability	753:773	TPS thermal stability	753:773	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	18	theme	PLA	745:747	arg1	intensity					646:654	intensity	646:654	intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms	646:729	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	18	theme	PLA	745:747	arg1	reduction					732:740	reduction	732:740	reduction of PLA	732:747	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	19	theme	glass	822:826	arg1	Tg					851:852	two glass transition temperature Tg	818:852	two glass transition temperature Tg	818:852	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	20	dep	metabolized	546:556	arg1	evidenced					808:816	evidenced	808:816	evidenced	808:816	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	2	21	theme	films	421:425	arg1	biodegradation-mineralization					337:365	the biodegradation-mineralization	333:365	the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions	333:478	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	0	22	dep	films	84:88	arg1	biodegradation					90:103	biodegradation	90:103	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	0	22	dep	films	84:88	arg1	approach					143:150	a novel, challenging and sustainable approach	106:150	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	3	23	theme	transition	828:837	arg1	Tg					851:852	two glass transition temperature Tg	818:852	two glass transition temperature Tg	818:852	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	2	24	theme	TPS	392:394	arg1	-poly					396:400	thermoplastic starch (TPS)-poly	370:400	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	24	theme	TPS	392:394	arg1	acid					409:412	lactic acid	402:412	lactic acid	402:412	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	24	theme	TPS	392:394	arg1	PLA					416:418	PLA	416:418	PLA	416:418	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	5	25	theme	Mineralization	1079:1092	arg1	tests					1094:1098	Mineralization tests	1079:1098	Mineralization tests evidenced UL	1079:1111	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	3	26	dep	evidenced	808:816	arg1	associated					875:884	associated	875:884	associated with depolymerized TPS and PLA, respectively	875:929	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	1	27	theme	Ulomoides	187:195	arg1	insects					238:244	macroinvertebrates insects	219:244	macroinvertebrates insects belonging to Tenebrionidae Coleopteran family	219:290	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	1	27	theme	Ulomoides	187:195	arg1	UL					211:212	UL	211:212	UL	211:212	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	1	27	theme	Ulomoides	187:195	arg1	dermestoides					197:208	Ulomoides dermestoides	187:208	Ulomoides dermestoides (UL)	187:213	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	3	28	theme	PLA	694:696	arg1	crystallinity					698:710	PLA crystallinity	694:710	PLA crystallinity by DSC thermograms	694:729	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	0	29	theme	challenging	115:125	arg1	approach					143:150	a novel, challenging and sustainable approach	106:150	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	0	30	theme	Coleopteran	23:33	arg1	action					35:40	Coleopteran action	23:40	Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	23:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	5	31	theme	challenging	1215:1225	arg1	perspective					1227:1237	a challenging perspective	1213:1237	a challenging perspective for end-life management of bioplastics	1213:1276	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	0	32	theme	Thermoplastic	45:57	arg1	Starch/Poly					59:69	Thermoplastic Starch/Poly	45:69	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	0	32	theme	Thermoplastic	45:57	arg1	acid					78:81	lactic acid	71:81	lactic acid	71:81	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	3	33	theme	%	689:689	arg1	spectra					664:670	FTIR spectra	659:670	FTIR spectra	659:670	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	0	34	theme	sustainable	131:141	arg1	approach					143:150	a novel, challenging and sustainable approach	106:150	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	5	35	theme	PLA	1173:1175	arg1	biodisintegration					1177:1193	PLA biodisintegration	1173:1193	PLA biodisintegration (50%)	1173:1199	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	5	35	theme	PLA	1173:1175	arg1	%					1198:1198	50%	1196:1198	50%	1196:1198	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	4	36	theme	PLA	1032:1034	arg1	concentration					1036:1048	PLA concentration	1032:1048	PLA concentration	1032:1048	SEM micrographs highlighted consumption of TPS-PLA surface, while GPC analysis showed a decrease in PLA concentration by 20% during contact by UL.					
34980348	2	37	theme	lactic	402:407	arg1	-poly					396:400	thermoplastic starch (TPS)-poly	370:400	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	37	theme	lactic	402:407	arg1	acid					409:412	lactic acid	402:412	lactic acid	402:412	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	38	theme	biodegradable	438:450	arg1	films					421:425	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	3	39	theme	DSC	715:717	arg1	thermograms					719:729	DSC thermograms	715:729	DSC thermograms	715:729	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	5	40	from	effect	1121:1126	arg1	biodisintegration					1177:1193	PLA biodisintegration	1173:1193	PLA biodisintegration (50%)	1173:1199	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	5	40	from	effect	1121:1126	arg1	%					1198:1198	50%	1196:1198	50%	1196:1198	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	5	40	from	effect	1121:1126	arg1	biodigestion-biodegradation					1135:1161	TPS biodigestion-biodegradation	1131:1161	TPS biodigestion-biodegradation (80%)	1131:1167	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	5	40	from	effect	1121:1126	arg1	%					1166:1166	80%	1164:1166	80%	1164:1166	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	4	41	from	decrease	1020:1027	arg1	concentration					1036:1048	PLA concentration	1032:1048	PLA concentration	1032:1048	SEM micrographs highlighted consumption of TPS-PLA surface, while GPC analysis showed a decrease in PLA concentration by 20% during contact by UL.					
34980348	3	42	with	contact	491:497	arg1	UL					520:521	UL	520:521	UL for five days	520:535	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	0	43	theme	Starch/Poly	59:69	arg1	films					84:88	Thermoplastic Starch/Poly(lactic acid) films	45:88	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	3	44	theme	crystallinity	698:710	arg1	%					689:689	13%	687:689	13% of PLA crystallinity by DSC thermograms	687:729	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	44	theme	crystallinity	698:710	arg1	crystallinity					698:710	PLA crystallinity	694:710	PLA crystallinity by DSC thermograms	694:729	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	45	theme	carbonyl	625:632	arg1	group					634:638	hydroxyl and carbonyl group	612:638	group	634:638	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	0	46	theme	fast	158:161	arg1	process					178:184	a fast mineralization process	156:184	a fast mineralization process	156:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	4	47	theme	GPC	998:1000	arg1	analysis					1002:1009	GPC analysis	998:1009	GPC analysis	998:1009	SEM micrographs highlighted consumption of TPS-PLA surface, while GPC analysis showed a decrease in PLA concentration by 20% during contact by UL.					
34980348	3	48	theme	hydroxyl	612:619	arg1	group					634:638	hydroxyl and carbonyl group	612:638	group	634:638	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	3	49	theme	TPS-PLA	502:508	arg1	film					510:513	TPS-PLA film	502:513	TPS-PLA film	502:513	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	1	50	theme	macroinvertebrates	219:236	arg1	insects					238:244	macroinvertebrates insects	219:244	macroinvertebrates insects belonging to Tenebrionidae Coleopteran family	219:290	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	1	50	theme	macroinvertebrates	219:236	arg1	dermestoides					197:208	Ulomoides dermestoides	187:208	Ulomoides dermestoides (UL)	187:213	Ulomoides dermestoides (UL) are macroinvertebrates insects belonging to Tenebrionidae Coleopteran family.					
34980348	5	51	theme	bioplastics	1266:1276	arg1	management					1252:1261	end-life management	1243:1261	end-life management of bioplastics	1243:1276	Mineralization tests evidenced UL boosted effect on TPS biodigestion-biodegradation (80%) and PLA biodisintegration (50%), envisaging a challenging perspective for end-life management of bioplastics in environmental conditions.					
34980348	2	52	theme	starch	384:389	arg1	-poly					396:400	thermoplastic starch (TPS)-poly	370:400	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	52	theme	starch	384:389	arg1	acid					409:412	lactic acid	402:412	lactic acid	402:412	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	52	theme	starch	384:389	arg1	PLA					416:418	PLA	416:418	PLA	416:418	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	3	53	theme	TGA	778:780	arg1	analysis					782:789	TGA analysis	778:789	TGA analysis	778:789	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	0	54	theme	lactic	71:76	arg1	Starch/Poly					59:69	Thermoplastic Starch/Poly	45:69	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	0	54	theme	lactic	71:76	arg1	acid					78:81	lactic acid	71:81	lactic acid	71:81	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	0	55	from	action	35:40	arg1	films					84:88	Thermoplastic Starch/Poly(lactic acid) films	45:88	Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process	45:184	Ulomoides dermestoides Coleopteran action on Thermoplastic Starch/Poly(lactic acid) films biodegradation: a novel, challenging and sustainable approach for a fast mineralization process.					
34980348	3	56	theme	depolymerized	891:903	arg1	TPS					905:907	depolymerized TPS	891:907	depolymerized TPS	891:907	After the contact of TPS-PLA film with UL for five days, TPS was metabolized and PLA was hydrolysed, as evidenced by decreasing of hydroxyl and carbonyl group peaks intensity by FTIR spectra, increasing of 13% of PLA crystallinity by DSC thermograms, reduction of PLA and TPS thermal stability by TGA analysis; faecal residues evidenced two glass transition temperature Tg, at 33 °C and 57 °C, associated with depolymerized TPS and PLA, respectively.					
34980348	2	57	theme	thermoplastic	370:382	arg1	-poly					396:400	thermoplastic starch (TPS)-poly	370:400	thermoplastic starch (TPS)-poly(lactic acid) (PLA) films	370:425	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	57	theme	thermoplastic	370:382	arg1	acid					409:412	lactic acid	402:412	lactic acid	402:412	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	2	57	theme	thermoplastic	370:382	arg1	PLA					416:418	PLA	416:418	PLA	416:418	They were used to hasten, in five days, the biodegradation-mineralization of thermoplastic starch (TPS)-poly(lactic acid) (PLA) films, otherwise biodegradable under composting conditions.					
34980348	4	58	theme	surface	983:989	arg1	consumption					960:970	consumption	960:970	consumption of TPS-PLA surface	960:989	SEM micrographs highlighted consumption of TPS-PLA surface, while GPC analysis showed a decrease in PLA concentration by 20% during contact by UL.					
33609630	0	0	theme	microbiome	76:85	arg1	composition					87:97	microbiome composition	76:97	microbiome composition	76:97	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	3	1	theme	bioactive	627:635	arg1	proteins					637:644	bioactive proteins	627:644	bioactive proteins	627:644	Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins individually and in combination for four weeks.					
33609630	2	2	theme	fraction	433:440	arg1	mix					370:372	a mix	368:372	a mix of probiotics, a mix of prebiotics and a bioactive protein fraction	368:440	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	7	3	theme	fecal	1085:1089	arg1	SCFAs					1091:1095	fecal SCFAs	1085:1095	fecal SCFAs	1085:1095	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	4	4	from	effects	708:714	arg1	composition					723:733	the composition	719:733	the composition of the gut microbiome	719:755	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	4	from	effects	708:714	arg1	inflammation					817:828	gut inflammation	813:828	gut inflammation	813:828	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	4	from	effects	708:714	arg1	concentration					794:806	gut short-chain fatty acids (SCFAs) concentration	758:806	gut short-chain fatty acids (SCFAs) concentration	758:806	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	1	5	theme	metabolic	160:168	arg1	activity					170:177	metabolic activity	160:177	metabolic activity	160:177	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	1	6	theme	current	241:247	arg1	interest					249:256	current interest	241:256	current interest in dietary constituents that may affect this system	241:308	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	0	7	from	Effect	0:5	arg1	composition					87:97	microbiome composition	76:97	microbiome composition	76:97	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	0	7	from	Effect	0:5	arg1	calprotectin					109:120	fecal calprotectin	103:120	fecal calprotectin	103:120	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	2	8	theme	study	331:335	arg1	purpose					315:321	The purpose	311:321	The purpose of this study	311:335	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	7	9	theme	gut	1139:1141	arg1	inflammation					1143:1154	gut inflammation	1139:1154	gut inflammation	1139:1154	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	7	10	theme	cecal	1076:1080	arg1	levels					1097:1102	cecal or fecal SCFAs levels	1076:1102	cecal or fecal SCFAs levels	1076:1102	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	5	11	theme	gut	887:889	arg1	microbiome					891:900	gut microbiome	887:900	gut microbiome	887:900	Ruminococcus gnavus was increased in mice gut microbiome after feeding prebiotics.					
33609630	4	12	theme	short-chain	762:772	arg1	SCFAs					787:791	SCFAs	787:791	SCFAs	787:791	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	12	theme	short-chain	762:772	arg1	acids					780:784	gut short-chain fatty acids	758:784	gut short-chain fatty acids (SCFAs) concentration	758:806	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	2	13	theme	probiotics	377:386	arg1	mix					370:372	a mix	368:372	a mix of probiotics, a mix of prebiotics and a bioactive protein fraction	368:440	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	0	14	theme	fecal	103:107	arg1	calprotectin					109:120	fecal calprotectin	103:120	fecal calprotectin	103:120	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	2	15	theme	protein	425:431	arg1	fraction					433:440	a bioactive protein fraction	413:440	a bioactive protein fraction	413:440	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	0	16	dep	composition	87:97	arg1	the					72:74	the	72:74	the	72:74	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	2	17	theme	prebiotics	398:407	arg1	fraction					433:440	a bioactive protein fraction	413:440	a bioactive protein fraction	413:440	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	2	17	theme	prebiotics	398:407	arg1	probiotics					377:386	probiotics	377:386	probiotics	377:386	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	2	17	theme	prebiotics	398:407	arg1	mix					391:393	a mix	389:393	a mix of prebiotics	389:407	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	4	18	theme	acids	780:784	arg1	concentration					794:806	gut short-chain fatty acids (SCFAs) concentration	758:806	gut short-chain fatty acids (SCFAs) concentration	758:806	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	1	19	theme	dietary	261:267	arg1	constituents					269:280	dietary constituents	261:280	dietary constituents that may affect this system	261:308	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	4	20	theme	fatty	774:778	arg1	SCFAs					787:791	SCFAs	787:791	SCFAs	787:791	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	20	theme	fatty	774:778	arg1	acids					780:784	gut short-chain fatty acids	758:784	gut short-chain fatty acids (SCFAs) concentration	758:806	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	7	21	theme	treatment	1055:1063	arg1	effects					1065:1071	minor treatment effects	1049:1071	minor treatment effects on cecal or fecal SCFAs levels	1049:1102	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	1	22	theme	microbiome	186:195	arg1	composition					144:154	composition	144:154	composition	144:154	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	1	22	theme	microbiome	186:195	arg1	activity					170:177	metabolic activity	160:177	metabolic activity	160:177	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	0	23	theme	prebiotics	23:32	arg1	Effect					0:5	Effect	0:5	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.	0:138	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	2	24	from	doses	531:535	arg1	combination					491:501	combination	491:501	combination at physiologically relevant doses	491:535	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	0	25	theme	C57BL6/j	125:132	arg1	mice					134:137	C57BL6/j mice	125:137	C57BL6/j mice	125:137	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	4	26	theme	microbiome	746:755	arg1	composition					723:733	the composition	719:733	the composition of the gut microbiome	719:755	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	26	theme	microbiome	746:755	arg1	inflammation					817:828	gut inflammation	813:828	gut inflammation	813:828	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	26	theme	microbiome	746:755	arg1	concentration					794:806	gut short-chain fatty acids (SCFAs) concentration	758:806	gut short-chain fatty acids (SCFAs) concentration	758:806	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	0	27	theme	supplemental	10:21	arg1	prebiotics					23:32	supplemental prebiotics	10:32	supplemental prebiotics	10:32	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	1	28	dep	composition	144:154	arg1	The					140:142	The	140:142	The	140:142	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	1	29	theme	many	204:207	arg1	aspects					209:215	many aspects	204:215	many aspects of health	204:225	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	0	30	theme	probiotics	35:44	arg1	Effect					0:5	Effect	0:5	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.	0:138	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	7	31	from	effects	1065:1071	arg1	levels					1097:1102	cecal or fecal SCFAs levels	1076:1102	cecal or fecal SCFAs levels	1076:1102	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	4	32	theme	gut	742:744	arg1	microbiome					746:755	the gut microbiome	738:755	the gut microbiome	738:755	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	7	33	theme	SCFAs	1091:1095	arg1	levels					1097:1102	cecal or fecal SCFAs levels	1076:1102	cecal or fecal SCFAs levels	1076:1102	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	2	34	theme	bioactive	415:423	arg1	fraction					433:440	a bioactive protein fraction	413:440	a bioactive protein fraction	413:440	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	2	35	theme	mix	391:393	arg1	mix					370:372	a mix	368:372	a mix of probiotics, a mix of prebiotics and a bioactive protein fraction	368:440	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	2	36	from	effects	357:363	arg1	microbiome					449:458	the microbiome	445:458	the microbiome	445:458	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	2	37	theme	mix	370:372	arg1	effects					357:363	the effects	353:363	the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome	353:458	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	0	38	theme	bioactive	50:58	arg1	proteins					60:67	bioactive proteins	50:67	bioactive proteins	50:67	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	2	39	theme	relevant	522:529	arg1	doses					531:535	physiologically relevant doses	506:535	physiologically relevant doses	506:535	The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses.					
33609630	5	40	dep	microbiome	891:900	arg1	feeding					908:914	feeding	908:914	feeding prebiotics	908:925	Ruminococcus gnavus was increased in mice gut microbiome after feeding prebiotics.					
33609630	1	41	from	interest	249:256	arg1	constituents					269:280	dietary constituents	261:280	dietary constituents that may affect this system	261:308	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	0	42	from	calprotectin	109:120	arg1	mice					134:137	C57BL6/j mice	125:137	C57BL6/j mice	125:137	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	4	43	theme	gut	758:760	arg1	SCFAs					787:791	SCFAs	787:791	SCFAs	787:791	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	4	43	theme	gut	758:760	arg1	acids					780:784	gut short-chain fatty acids	758:784	gut short-chain fatty acids (SCFAs) concentration	758:806	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	3	44	theme	total	556:560	arg1	TWD					576:578	TWD	576:578	TWD	576:578	Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins individually and in combination for four weeks.					
33609630	3	44	theme	total	556:560	arg1	diet					570:573	the total western diet	552:573	the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins	552:644	Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins individually and in combination for four weeks.					
33609630	1	45	theme	health	220:225	arg1	aspects					209:215	many aspects	204:215	many aspects of health	204:225	The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system.					
33609630	4	46	theme	gut	813:815	arg1	inflammation					817:828	gut inflammation	813:828	gut inflammation	813:828	Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured.					
33609630	3	47	theme	western	562:568	arg1	TWD					576:578	TWD	576:578	TWD	576:578	Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins individually and in combination for four weeks.					
33609630	3	47	theme	western	562:568	arg1	diet					570:573	the total western diet	552:573	the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins	552:644	Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins individually and in combination for four weeks.					
33609630	5	48	theme	Ruminococcus	845:856	arg1	gnavus					858:863	Ruminococcus gnavus	845:863	Ruminococcus gnavus	845:863	Ruminococcus gnavus was increased in mice gut microbiome after feeding prebiotics.					
33609630	7	49	theme	minor	1049:1053	arg1	effects					1065:1071	minor treatment effects	1049:1071	minor treatment effects on cecal or fecal SCFAs levels	1049:1102	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	7	50	theme	fecal	1171:1175	arg1	calprotectin					1177:1188	fecal calprotectin	1171:1188	fecal calprotectin	1171:1188	The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.					
33609630	0	51	from	composition	87:97	arg1	mice					134:137	C57BL6/j mice	125:137	C57BL6/j mice	125:137	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
33609630	0	52	theme	proteins	60:67	arg1	Effect					0:5	Effect	0:5	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.	0:138	Effect of supplemental prebiotics, probiotics and bioactive proteins on the microbiome composition and fecal calprotectin in C57BL6/j mice.					
32621318	6	0	theme	cellular	1023:1030	arg1	composition					1032:1042	cellular composition	1023:1042	cellular composition of BAL, lung, and spleen	1023:1067	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	0	1	theme	asthma	184:189	arg1	model					166:170	a mouse model	158:170	a mouse model of allergic asthma	158:189	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	3	2	theme	therapeutic	595:605	arg1	efficacy					607:614	therapeutic efficacy	595:614	therapeutic efficacy	595:614	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	12	3	with	SCIT	1966:1969	arg1	alum					1976:1979	alum	1976:1979	alum	1976:1979	LamOVA EPIT via laser-generated micropores is safe and equally effective compared to SCIT with alum, without the need for adjuvant.					
32621318	4	4	theme	LamOVA	703:708	arg1	activity					691:698	The biological activity	676:698	The biological activity of LamOVA	676:708	METHODS The biological activity of LamOVA was characterized in vitro using bone marrow-derived dendritic cells.					
32621318	11	5	theme	ovalbumin	1777:1785	arg1	Conjugation					1762:1772	CONCLUSION Conjugation	1751:1772	CONCLUSION Conjugation of ovalbumin to laminarin	1751:1798	CONCLUSION Conjugation of ovalbumin to laminarin increased its immunogenicity while at the same time reducing local side effects.					
32621318	9	6	theme	lung	1505:1508	arg1	inflammation					1510:1521	lung inflammation	1505:1521	lung inflammation	1505:1521	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	12	7	theme	LamOVA	1881:1886	arg1	EPIT					1888:1891	LamOVA EPIT	1881:1891	LamOVA EPIT via laser-generated micropores	1881:1922	LamOVA EPIT via laser-generated micropores is safe and equally effective compared to SCIT with alum, without the need for adjuvant.					
32621318	3	8	theme	neoglycoconjugates	639:656	arg1	allergenicity					576:588	allergenicity	576:588	allergenicity	576:588	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	3	8	theme	neoglycoconjugates	639:656	arg1	immunogenicity					560:573	immunogenicity	560:573	immunogenicity	560:573	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	3	8	theme	neoglycoconjugates	639:656	arg1	efficacy					607:614	therapeutic efficacy	595:614	therapeutic efficacy	595:614	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	1	9	from	cells	292:296	arg1	rich					265:268	rich	265:268	rich	265:268	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	10	10	theme	skin	1691:1694	arg1	reactions					1696:1704	significantly lower local skin reactions	1665:1704	significantly lower local skin reactions	1665:1704	EPIT with LamOVA induced significantly lower local skin reactions during therapy compared to unconjugated OVA.					
32621318	7	11	theme	IgE	1182:1184	arg1	capacity					1194:1201	its IgE binding capacity	1178:1201	its IgE binding capacity	1178:1201	RESULTS Conjugation of laminarin to ovalbumin reduced its IgE binding capacity fivefold and increased its immunogenicity threefold in terms of IgG generation.					
32621318	6	12	theme	infrared	915:922	arg1	ablation					930:937	fractional infrared laser ablation	904:937	fractional infrared laser ablation	904:937	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	9	13	theme	hyperresponsiveness	1534:1552	arg1	suppression					1490:1500	suppression	1490:1500	suppression of lung inflammation and airway hyperresponsiveness	1490:1552	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	9	13	theme	hyperresponsiveness	1534:1552	arg1	induction					1476:1484	IgG induction	1472:1484	IgG induction	1472:1484	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	6	14	theme	spleen	1062:1067	arg1	IgG					1013:1015	blocking IgG	1004:1015	blocking IgG	1004:1015	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	14	theme	spleen	1062:1067	arg1	polarization					1096:1107	T-cell polarization	1089:1107	T-cell polarization	1089:1107	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	14	theme	spleen	1062:1067	arg1	function					1075:1082	lung function	1070:1082	lung function	1070:1082	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	14	theme	spleen	1062:1067	arg1	IgE					1018:1020	IgE	1018:1020	IgE	1018:1020	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	14	theme	spleen	1062:1067	arg1	composition					1032:1042	cellular composition	1023:1042	cellular composition of BAL, lung, and spleen	1023:1067	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	9	15	theme	inflammation	1510:1521	arg1	suppression					1490:1500	suppression	1490:1500	suppression of lung inflammation and airway hyperresponsiveness	1490:1552	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	9	15	theme	inflammation	1510:1521	arg1	induction					1476:1484	IgG induction	1472:1484	IgG induction	1472:1484	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	10	16	theme	lower	1679:1683	arg1	reactions					1696:1704	significantly lower local skin reactions	1665:1704	significantly lower local skin reactions	1665:1704	EPIT with LamOVA induced significantly lower local skin reactions during therapy compared to unconjugated OVA.					
32621318	5	17	theme	BALB/c	837:842	arg1	mice					844:847	BALB/c mice	837:847	BALB/c mice	837:847	Immunogenicity and therapeutic efficacy were analyzed in BALB/c mice.					
32621318	8	18	theme	higher	1322:1327	arg1	levels					1333:1338	significantly higher IgG levels	1308:1338	significantly higher IgG levels	1308:1338	EPIT with LamOVA induced significantly higher IgG levels than OVA, matching the levels induced by s.c. injection of OVA/alum (SCIT).					
32621318	2	19	theme	activating	450:459	arg1	cells					471:475	activating dendritic cells	450:475	activating dendritic cells	450:475	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	4	20	theme	dendritic	763:771	arg1	cells					773:777	bone marrow-derived dendritic cells	743:777	bone marrow-derived dendritic cells	743:777	METHODS The biological activity of LamOVA was characterized in vitro using bone marrow-derived dendritic cells.					
32621318	0	21	theme	allergic	175:182	arg1	asthma					184:189	allergic asthma	175:189	allergic asthma	175:189	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	8	22	with	EPIT	1283:1286	arg1	LamOVA					1293:1298	LamOVA	1293:1298	LamOVA	1293:1298	EPIT with LamOVA induced significantly higher IgG levels than OVA, matching the levels induced by s.c. injection of OVA/alum (SCIT).					
32621318	8	23	theme	OVA/alum	1399:1406	arg1	injection					1386:1394	s.c. injection	1381:1394	s.c. injection of OVA/alum (SCIT)	1381:1413	EPIT with LamOVA induced significantly higher IgG levels than OVA, matching the levels induced by s.c. injection of OVA/alum (SCIT).					
32621318	11	24	theme	local	1861:1865	arg1	effects					1872:1878	local side effects	1861:1878	local side effects	1861:1878	CONCLUSION Conjugation of ovalbumin to laminarin increased its immunogenicity while at the same time reducing local side effects.					
32621318	7	25	theme	RESULTS	1124:1130	arg1	Conjugation					1132:1142	RESULTS Conjugation	1124:1142	RESULTS Conjugation of laminarin to ovalbumin	1124:1168	RESULTS Conjugation of laminarin to ovalbumin reduced its IgE binding capacity fivefold and increased its immunogenicity threefold in terms of IgG generation.					
32621318	0	26	theme	lung	107:110	arg1	inflammation					112:123	lung inflammation	107:123	lung inflammation	107:123	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	9	27	theme	therapy-induced	1601:1615	arg1	IgE					1617:1619	therapy-induced IgE	1601:1619	therapy-induced IgE	1601:1619	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	6	28	theme	T-cell	1089:1094	arg1	polarization					1096:1107	T-cell polarization	1089:1107	T-cell polarization	1089:1107	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	9	29	from	terms	1454:1458	arg1	effective					1433:1441	effective	1433:1441	effective	1433:1441	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	1	30	theme	BACKGROUND	192:201	arg1	immunotherapy					221:233	BACKGROUND Allergen-specific immunotherapy	192:233	BACKGROUND Allergen-specific immunotherapy via the skin	192:246	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	4	31	link	marrow-derived	748:761	arg1	cells					773:777	bone marrow-derived dendritic cells	743:777	bone marrow-derived dendritic cells	743:777	METHODS The biological activity of LamOVA was characterized in vitro using bone marrow-derived dendritic cells.					
32621318	0	32	theme	local	136:140	arg1	effects					147:153	local side effects	136:153	local side effects	136:153	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	9	33	theme	cytokines	1629:1637	arg1	levels					1591:1596	higher levels	1584:1596	higher levels of therapy-induced IgE and TH2 cytokines	1584:1637	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	6	34	theme	lung	1070:1073	arg1	function					1075:1082	lung function	1070:1082	lung function	1070:1082	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	9	35	from	effective	1433:1441	arg1	terms					1454:1458	terms	1454:1458	terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness	1454:1552	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	2	36	dep	neoglycogonjugates	383:400	arg1	increase					402:409	increase	402:409	increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors	402:503	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	2	36	dep	neoglycogonjugates	383:400	arg1	reduce					509:514	reduce	509:514	reduce side effects	509:527	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	2	37	theme	dendritic	461:469	arg1	cells					471:475	activating dendritic cells	450:475	activating dendritic cells	450:475	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	0	38	theme	Laser-facilitated	0:16	arg1	immunotherapy					31:43	Laser-facilitated epicutaneous immunotherapy	0:43	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates	0:94	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	6	39	from	micropores	951:960	arg1	skin					969:972	the skin	965:972	the skin	965:972	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	2	40	theme	side	516:519	arg1	effects					521:527	side effects	516:527	side effects	516:527	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	7	41	theme	IgG	1267:1269	arg1	generation					1271:1280	IgG generation	1267:1280	IgG generation	1267:1280	RESULTS Conjugation of laminarin to ovalbumin reduced its IgE binding capacity fivefold and increased its immunogenicity threefold in terms of IgG generation.					
32621318	1	42	theme	local	326:330	arg1	effects					350:356	local and systemic side effects	326:356	local and systemic side effects	326:356	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	6	43	theme	LamOVA	994:999	arg1	effects					983:989	the effects	979:989	the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization	979:1107	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	2	44	theme	lectin	488:493	arg1	receptors					495:503	C-type lectin receptors	481:503	C-type lectin receptors	481:503	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	0	45	theme	hypoallergenic	50:63	arg1	neoglycoconjugates					77:94	hypoallergenic beta-glucan neoglycoconjugates	50:94	hypoallergenic beta-glucan neoglycoconjugates	50:94	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	1	46	theme	systemic	336:343	arg1	effects					350:356	local and systemic side effects	326:356	local and systemic side effects	326:356	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	0	47	with	immunotherapy	31:43	arg1	neoglycoconjugates					77:94	hypoallergenic beta-glucan neoglycoconjugates	50:94	hypoallergenic beta-glucan neoglycoconjugates	50:94	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	2	48	theme	immunization	411:422	arg1	efficacy					424:431	immunization efficacy	411:431	immunization efficacy	411:431	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	11	49	theme	same	1842:1845	arg1	time					1847:1850	the same time	1838:1850	the same time	1838:1850	CONCLUSION Conjugation of ovalbumin to laminarin increased its immunogenicity while at the same time reducing local side effects.					
32621318	1	50	theme	rich	265:268	arg1	tissue					258:263	a tissue	256:263	a tissue rich in antigen-presenting cells	256:296	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	12	51	theme	laser-generated	1897:1911	arg1	micropores					1913:1922	laser-generated micropores	1897:1922	laser-generated micropores	1897:1922	LamOVA EPIT via laser-generated micropores is safe and equally effective compared to SCIT with alum, without the need for adjuvant.					
32621318	9	52	theme	IgG	1472:1474	arg1	induction					1476:1484	IgG induction	1472:1484	IgG induction	1472:1484	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	11	53	theme	CONCLUSION	1751:1760	arg1	Conjugation					1762:1772	CONCLUSION Conjugation	1751:1772	CONCLUSION Conjugation of ovalbumin to laminarin	1751:1798	CONCLUSION Conjugation of ovalbumin to laminarin increased its immunogenicity while at the same time reducing local side effects.					
32621318	6	54	theme	Epicutaneous	850:861	arg1	EPIT					878:881	EPIT	878:881	EPIT	878:881	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	54	theme	Epicutaneous	850:861	arg1	immunotherapy					863:875	Epicutaneous immunotherapy	850:875	Epicutaneous immunotherapy (EPIT)	850:882	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	4	55	theme	biological	680:689	arg1	activity					691:698	The biological activity	676:698	The biological activity of LamOVA	676:708	METHODS The biological activity of LamOVA was characterized in vitro using bone marrow-derived dendritic cells.					
32621318	10	56	with	EPIT	1640:1643	arg1	LamOVA					1650:1655	LamOVA	1650:1655	LamOVA	1650:1655	EPIT with LamOVA induced significantly lower local skin reactions during therapy compared to unconjugated OVA.					
32621318	7	57	theme	binding	1186:1192	arg1	capacity					1194:1201	its IgE binding capacity	1178:1201	its IgE binding capacity	1178:1201	RESULTS Conjugation of laminarin to ovalbumin reduced its IgE binding capacity fivefold and increased its immunogenicity threefold in terms of IgG generation.					
32621318	3	58	theme	laminarin-ovalbumin	619:637	arg1	LamOVA					659:664	LamOVA	659:664	LamOVA	659:664	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	3	58	theme	laminarin-ovalbumin	619:637	arg1	neoglycoconjugates					639:656	laminarin-ovalbumin neoglycoconjugates	619:656	laminarin-ovalbumin neoglycoconjugates (LamOVA)	619:665	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	1	59	theme	Allergen-specific	203:219	arg1	immunotherapy					221:233	BACKGROUND Allergen-specific immunotherapy	192:233	BACKGROUND Allergen-specific immunotherapy via the skin	192:246	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	6	60	theme	blocking	1004:1011	arg1	IgG					1013:1015	blocking IgG	1004:1015	blocking IgG	1004:1015	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	61	from	effects	983:989	arg1	IgG					1013:1015	blocking IgG	1004:1015	blocking IgG	1004:1015	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	61	from	effects	983:989	arg1	polarization					1096:1107	T-cell polarization	1089:1107	T-cell polarization	1089:1107	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	61	from	effects	983:989	arg1	function					1075:1082	lung function	1070:1082	lung function	1070:1082	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	61	from	effects	983:989	arg1	IgE					1018:1020	IgE	1018:1020	IgE	1018:1020	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	61	from	effects	983:989	arg1	composition					1032:1042	cellular composition	1023:1042	cellular composition of BAL, lung, and spleen	1023:1067	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	62	theme	laser	924:928	arg1	ablation					930:937	fractional infrared laser ablation	904:937	fractional infrared laser ablation	904:937	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	9	63	theme	airway	1527:1532	arg1	hyperresponsiveness					1534:1552	airway hyperresponsiveness	1527:1552	airway hyperresponsiveness	1527:1552	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	10	64	theme	local	1685:1689	arg1	reactions					1696:1704	significantly lower local skin reactions	1665:1704	significantly lower local skin reactions	1665:1704	EPIT with LamOVA induced significantly lower local skin reactions during therapy compared to unconjugated OVA.					
32621318	0	65	theme	mouse	160:164	arg1	model					166:170	a mouse model	158:170	a mouse model of allergic asthma	158:189	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	6	66	theme	fractional	904:913	arg1	ablation					930:937	fractional infrared laser ablation	904:937	fractional infrared laser ablation	904:937	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	7	67	theme	laminarin	1147:1155	arg1	Conjugation					1132:1142	RESULTS Conjugation	1124:1142	RESULTS Conjugation of laminarin to ovalbumin	1124:1168	RESULTS Conjugation of laminarin to ovalbumin reduced its IgE binding capacity fivefold and increased its immunogenicity threefold in terms of IgG generation.					
32621318	6	68	theme	lung	1052:1055	arg1	IgG					1013:1015	blocking IgG	1004:1015	blocking IgG	1004:1015	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	68	theme	lung	1052:1055	arg1	polarization					1096:1107	T-cell polarization	1089:1107	T-cell polarization	1089:1107	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	68	theme	lung	1052:1055	arg1	function					1075:1082	lung function	1070:1082	lung function	1070:1082	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	68	theme	lung	1052:1055	arg1	IgE					1018:1020	IgE	1018:1020	IgE	1018:1020	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	68	theme	lung	1052:1055	arg1	composition					1032:1042	cellular composition	1023:1042	cellular composition of BAL, lung, and spleen	1023:1067	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	4	69	theme	marrow-derived	748:761	arg1	cells					773:777	bone marrow-derived dendritic cells	743:777	bone marrow-derived dendritic cells	743:777	METHODS The biological activity of LamOVA was characterized in vitro using bone marrow-derived dendritic cells.					
32621318	5	70	theme	therapeutic	799:809	arg1	efficacy					811:818	therapeutic efficacy	799:818	therapeutic efficacy	799:818	Immunogenicity and therapeutic efficacy were analyzed in BALB/c mice.					
32621318	8	71	theme	IgG	1329:1331	arg1	levels					1333:1338	significantly higher IgG levels	1308:1338	significantly higher IgG levels	1308:1338	EPIT with LamOVA induced significantly higher IgG levels than OVA, matching the levels induced by s.c. injection of OVA/alum (SCIT).					
32621318	6	72	theme	BAL	1047:1049	arg1	IgG					1013:1015	blocking IgG	1004:1015	blocking IgG	1004:1015	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	72	theme	BAL	1047:1049	arg1	polarization					1096:1107	T-cell polarization	1089:1107	T-cell polarization	1089:1107	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	72	theme	BAL	1047:1049	arg1	function					1075:1082	lung function	1070:1082	lung function	1070:1082	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	72	theme	BAL	1047:1049	arg1	IgE					1018:1020	IgE	1018:1020	IgE	1018:1020	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	6	72	theme	BAL	1047:1049	arg1	composition					1032:1042	cellular composition	1023:1042	cellular composition of BAL, lung, and spleen	1023:1067	Epicutaneous immunotherapy (EPIT) was performed using fractional infrared laser ablation to generate micropores in the skin, and the effects of LamOVA on blocking IgG, IgE, cellular composition of BAL, lung, and spleen, lung function, and T-cell polarization were assessed.					
32621318	8	73	theme	s.c.	1381:1384	arg1	injection					1386:1394	s.c. injection	1381:1394	s.c. injection of OVA/alum (SCIT)	1381:1413	EPIT with LamOVA induced significantly higher IgG levels than OVA, matching the levels induced by s.c. injection of OVA/alum (SCIT).					
32621318	1	74	from	rich	265:268	arg1	cells					292:296	antigen-presenting cells	273:296	antigen-presenting cells	273:296	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	9	75	theme	higher	1584:1589	arg1	levels					1591:1596	higher levels	1584:1596	higher levels of therapy-induced IgE and TH2 cytokines	1584:1637	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	9	76	theme	TH2	1625:1627	arg1	cytokines					1629:1637	TH2 cytokines	1625:1637	TH2 cytokines	1625:1637	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	11	77	theme	side	1867:1870	arg1	effects					1872:1878	local side effects	1861:1878	local side effects	1861:1878	CONCLUSION Conjugation of ovalbumin to laminarin increased its immunogenicity while at the same time reducing local side effects.					
32621318	9	78	theme	IgE	1617:1619	arg1	levels					1591:1596	higher levels	1584:1596	higher levels of therapy-induced IgE and TH2 cytokines	1584:1637	EPIT was equally effective as SCIT in terms of blocking IgG induction and suppression of lung inflammation and airway hyperresponsiveness, but SCIT was associated with higher levels of therapy-induced IgE and TH2 cytokines.					
32621318	0	79	theme	side	142:145	arg1	effects					147:153	local side effects	136:153	local side effects	136:153	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	3	80	dep	immunogenicity	560:573	arg1	the					556:558	the	556:558	the	556:558	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	4	81	dep	METHODS	668:674	arg1	characterized					714:726	characterized	714:726	was characterized in vitro using bone marrow-derived dendritic cells	710:777	METHODS The biological activity of LamOVA was characterized in vitro using bone marrow-derived dendritic cells.					
32621318	0	82	theme	epicutaneous	18:29	arg1	immunotherapy					31:43	Laser-facilitated epicutaneous immunotherapy	0:43	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates	0:94	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	10	83	theme	unconjugated	1733:1744	arg1	OVA					1746:1748	unconjugated OVA	1733:1748	unconjugated OVA	1733:1748	EPIT with LamOVA induced significantly lower local skin reactions during therapy compared to unconjugated OVA.					
32621318	0	84	theme	beta-glucan	65:75	arg1	neoglycoconjugates					77:94	hypoallergenic beta-glucan neoglycoconjugates	50:94	hypoallergenic beta-glucan neoglycoconjugates	50:94	Laser-facilitated epicutaneous immunotherapy with hypoallergenic beta-glucan neoglycoconjugates suppresses lung inflammation and avoids local side effects in a mouse model of allergic asthma.					
32621318	1	85	theme	antigen-presenting	273:290	arg1	cells					292:296	antigen-presenting cells	273:296	antigen-presenting cells	273:296	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	2	86	theme	C-type	481:486	arg1	receptors					495:503	C-type lectin receptors	481:503	C-type lectin receptors	481:503	Allergen-polysaccharide neoglycogonjugates increase immunization efficacy by targeting and activating dendritic cells via C-type lectin receptors and reduce side effects.					
32621318	1	87	theme	side	345:348	arg1	effects					350:356	local and systemic side effects	326:356	local and systemic side effects	326:356	BACKGROUND Allergen-specific immunotherapy via the skin targets a tissue rich in antigen-presenting cells, but can be associated with local and systemic side effects.					
32621318	3	88	dep	OBJECTIVE	530:538	arg1	investigated					543:554	investigated	543:554	investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA)	543:665	OBJECTIVE We investigated the immunogenicity, allergenicity, and therapeutic efficacy of laminarin-ovalbumin neoglycoconjugates (LamOVA).					
32621318	7	89	theme	generation	1271:1280	arg1	terms					1258:1262	terms	1258:1262	terms of IgG generation	1258:1280	RESULTS Conjugation of laminarin to ovalbumin reduced its IgE binding capacity fivefold and increased its immunogenicity threefold in terms of IgG generation.					
33125406	2	0	theme	metabolic	338:346	arg1	characteristics					348:362	muscle metabolic characteristics	331:362	muscle metabolic characteristics	331:362	However, ketogenic diet effects on muscle metabolic characteristics are diverse and sometimes countervailing.					
33125406	4	1	theme	%	740:740	arg1	protein					742:748	10% protein	738:748	10% protein	738:748	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	1	theme	%	740:740	arg1	fat					733:735	90% fat	729:735	90% fat	729:735	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	6	2	theme	metabolic	988:996	arg1	enzymes					998:1004	metabolic enzymes	988:1004	metabolic enzymes	988:1004	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	10	3	theme	skeletal	1540:1547	arg1	muscle					1549:1554	skeletal muscle	1540:1554	skeletal muscle aerobic capacity	1540:1571	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	1	4	theme	metabolism	254:263	arg1	properties					265:274	the energy metabolism properties	243:274	the energy metabolism properties of skeletal muscle	243:293	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	4	5	theme	%	731:731	arg1	protein					742:748	10% protein	738:748	10% protein	738:748	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	5	theme	%	731:731	arg1	carbohydrate					754:765	0% carbohydrate	751:765	0% carbohydrate	751:765	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	5	theme	%	731:731	arg1	fat					733:735	90% fat	729:735	90% fat	729:735	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	6	6	theme	chain	947:951	arg1	composition					953:963	the myosin heavy chain composition	930:963	the myosin heavy chain composition	930:963	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	10	7	theme	aerobic	1556:1562	arg1	capacity					1564:1571	skeletal muscle aerobic capacity	1540:1571	skeletal muscle aerobic capacity	1540:1571	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	9	8	theme	type	1450:1453	arg1	IIb					1455:1457	type IIb	1450:1457	type IIb	1450:1457	Moreover, a myosin heavy chain shift occurred from type IIb to IIx in KD.					
33125406	9	9	from	IIx	1462:1464	arg1	KD					1469:1470	KD	1469:1470	KD	1469:1470	Moreover, a myosin heavy chain shift occurred from type IIb to IIx in KD.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	synthase					1251:1258	citrate synthase	1243:1258	citrate synthase	1243:1258	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	dehydrogenase					1340:1352	3-hydroxyacyl-CoA dehydrogenase	1322:1352	3-hydroxyacyl-CoA dehydrogenase	1322:1352	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	subunit					1307:1313	cytochrome c oxidase subunit 4	1286:1315	cytochrome c oxidase subunit 4	1286:1315	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	expression					1221:1230	the protein expression	1209:1230	the protein expression of Sema3A	1209:1240	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	higher					1373:1378	higher	1373:1378	higher	1373:1378	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	10	theme	Sema3A	1235:1240	arg1	dehydrogenase					1271:1283	succinate dehydrogenase	1261:1283	succinate dehydrogenase	1261:1283	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	6	11	theme	synthase	1042:1049	arg1	activity					1051:1058	citrate synthase activity	1034:1058	citrate synthase activity	1034:1058	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	8	12	theme	citrate	1243:1249	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	12	theme	citrate	1243:1249	arg1	synthase					1251:1258	citrate synthase	1243:1258	citrate synthase	1243:1258	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	10	13	theme	muscle	1593:1598	arg1	function					1612:1619	muscle contractile function	1593:1619	muscle contractile function in sedentary male rats	1593:1642	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	1	14	theme	Recent	120:125	arg1	studies					127:133	Recent studies	120:133	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates,	120:217	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	4	15	theme	%	752:752	arg1	fat					733:735	90% fat	729:735	90% fat	729:735	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	15	theme	%	752:752	arg1	carbohydrate					754:765	0% carbohydrate	751:765	0% carbohydrate	751:765	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	6	16	theme	factors	1021:1027	arg1	composition					953:963	the myosin heavy chain composition	930:963	the myosin heavy chain composition	930:963	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	6	16	theme	factors	1021:1027	arg1	expression					974:983	protein expression	966:983	protein expression of metabolic enzymes and regulatory factors	966:1027	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	6	16	theme	factors	1021:1027	arg1	activity					1051:1058	citrate synthase activity	1034:1058	citrate synthase activity	1034:1058	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	5	17	theme	twitch	871:876	arg1	force					878:882	twitch force	871:882	twitch force	871:882	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	0	18	theme	extensor	63:70	arg1	longus					82:87	extensor digitorum longus	63:87	extensor digitorum longus muscle of sedentary male rats	63:117	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	10	19	theme	sedentary	1624:1632	arg1	rats					1639:1642	sedentary male rats	1624:1642	sedentary male rats	1624:1642	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	1	20	theme	ketogenic	142:150	arg1	diet					180:183	an extremely high-fat diet	158:183	an extremely high-fat diet with extremely low carbohydrates	158:216	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	1	20	theme	ketogenic	142:150	arg1	diet					152:155	the ketogenic diet	138:155	the ketogenic diet	138:155	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	8	21	theme	cytochrome	1286:1295	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	21	theme	cytochrome	1286:1295	arg1	subunit					1307:1313	cytochrome c oxidase subunit 4	1286:1315	cytochrome c oxidase subunit 4	1286:1315	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	0	22	theme	longus	82:87	arg1	muscle					89:94	extensor digitorum longus muscle	63:94	extensor digitorum longus muscle of sedentary male rats	63:117	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	8	23	theme	oxidase	1299:1305	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	23	theme	oxidase	1299:1305	arg1	subunit					1307:1313	cytochrome c oxidase subunit 4	1286:1315	cytochrome c oxidase subunit 4	1286:1315	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	4	24	theme	10	676:677	arg1	%					678:678	%	678:678	%	678:678	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	3	25	theme	skeletal	445:452	arg1	performance					461:471	skeletal muscle performance	445:471	skeletal muscle performance	445:471	Furthermore, ketogenic diet effects on skeletal muscle performance are unknown.					
33125406	0	26	theme	male	109:112	arg1	rats					114:117	sedentary male rats	99:117	sedentary male rats	99:117	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	5	27	theme	digitorum	815:823	arg1	EDL					833:835	EDL	833:835	EDL	833:835	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	5	27	theme	digitorum	815:823	arg1	longus					825:830	extensor digitorum longus	806:830	the extensor digitorum longus (EDL) muscle	802:843	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	4	28	theme	Wistar	497:502	arg1	rats					504:507	male Wistar rats	492:507	male Wistar rats (8 weeks of age)	492:524	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	29	theme	10	667:668	arg1	%					669:669	%	669:669	%	669:669	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	0	30	theme	Ketogenic	0:8	arg1	feeding					15:21	Ketogenic diet feeding	0:21	Ketogenic diet feeding	0:21	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	4	31	dep	rats	504:507	arg1	weeks					512:516	8 weeks	510:516	8 weeks of age	510:523	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	5	32	theme	4-week	779:784	arg1	period					794:799	the 4-week feeding period	775:799	the 4-week feeding period	775:799	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	8	33	theme	KD	1176:1177	arg1	synthase					1187:1194	the KD citrate synthase	1172:1194	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	4	34	dep	diet	723:726	arg1	protein					742:748	10% protein	738:748	10% protein	738:748	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	34	dep	diet	723:726	arg1	carbohydrate					754:765	0% carbohydrate	751:765	0% carbohydrate	751:765	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	34	dep	diet	723:726	arg1	fat					733:735	90% fat	729:735	90% fat	729:735	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	1	35	theme	skeletal	279:286	arg1	muscle					288:293	skeletal muscle	279:293	skeletal muscle	279:293	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	3	36	theme	ketogenic	419:427	arg1	effects					434:440	ketogenic diet effects	419:440	ketogenic diet effects on skeletal muscle performance	419:471	Furthermore, ketogenic diet effects on skeletal muscle performance are unknown.					
33125406	8	37	theme	synthase	1187:1194	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	37	theme	synthase	1187:1194	arg1	dehydrogenase					1340:1352	3-hydroxyacyl-CoA dehydrogenase	1322:1352	3-hydroxyacyl-CoA dehydrogenase	1322:1352	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	37	theme	synthase	1187:1194	arg1	synthase					1251:1258	citrate synthase	1243:1258	citrate synthase	1243:1258	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	37	theme	synthase	1187:1194	arg1	dehydrogenase					1271:1283	succinate dehydrogenase	1261:1283	succinate dehydrogenase	1261:1283	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	37	theme	synthase	1187:1194	arg1	subunit					1307:1313	cytochrome c oxidase subunit 4	1286:1315	cytochrome c oxidase subunit 4	1286:1315	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	0	38	theme	aerobic	32:38	arg1	property					51:58	aerobic metabolism property	32:58	aerobic metabolism property	32:58	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	4	39	theme	age	521:523	arg1	weeks					512:516	8 weeks	510:516	8 weeks of age	510:523	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	40	theme	ketogenic	713:721	arg1	diet					723:726	a ketogenic diet	711:726	a ketogenic diet (90% fat, 10% protein, 0% carbohydrate)	711:766	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	2	41	theme	muscle	331:336	arg1	characteristics					348:362	muscle metabolic characteristics	331:362	muscle metabolic characteristics	331:362	However, ketogenic diet effects on muscle metabolic characteristics are diverse and sometimes countervailing.					
33125406	1	42	with	diet	180:183	arg1	carbohydrates					204:216	extremely low carbohydrates	190:216	extremely low carbohydrates	190:216	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	6	43	theme	enzymes	998:1004	arg1	composition					953:963	the myosin heavy chain composition	930:963	the myosin heavy chain composition	930:963	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	6	43	theme	enzymes	998:1004	arg1	expression					974:983	protein expression	966:983	protein expression of metabolic enzymes and regulatory factors	966:1027	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	6	43	theme	enzymes	998:1004	arg1	activity					1051:1058	citrate synthase activity	1034:1058	citrate synthase activity	1034:1058	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	10	44	theme	myosin	1685:1690	arg1	chain					1698:1702	the myosin heavy chain	1681:1702	the myosin heavy chain shift of EDL muscle	1681:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	8	45	theme	protein	1213:1219	arg1	higher					1373:1378	higher	1373:1378	higher	1373:1378	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	45	theme	protein	1213:1219	arg1	expression					1221:1230	the protein expression	1209:1230	the protein expression of Sema3A	1209:1240	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	9	46	theme	heavy	1418:1422	arg1	chain					1424:1428	myosin heavy chain	1411:1428	a myosin heavy chain shift	1409:1434	Moreover, a myosin heavy chain shift occurred from type IIb to IIx in KD.					
33125406	10	47	theme	ketogenic	1516:1524	arg1	diet					1526:1529	the 4-week ketogenic diet	1505:1529	the 4-week ketogenic diet	1505:1529	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	7	48	theme	significant	1064:1074	arg1	difference					1076:1085	No significant difference	1061:1085	No significant difference	1061:1085	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	4	49	theme	ketogenic	580:588	arg1	KD					602:603	KD	602:603	KD	602:603	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	49	theme	ketogenic	580:588	arg1	group					595:599	a ketogenic diet group	578:599	a ketogenic diet group (KD)	578:604	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	2	50	from	effects	320:326	arg1	characteristics					348:362	muscle metabolic characteristics	331:362	muscle metabolic characteristics	331:362	However, ketogenic diet effects on muscle metabolic characteristics are diverse and sometimes countervailing.					
33125406	10	51	theme	chain	1698:1702	arg1	shift					1704:1708	the myosin heavy chain shift	1681:1708	the myosin heavy chain shift of EDL muscle	1681:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	10	52	theme	muscle	1549:1554	arg1	capacity					1564:1571	skeletal muscle aerobic capacity	1540:1571	skeletal muscle aerobic capacity	1540:1571	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	1	53	theme	energy	247:252	arg1	properties					265:274	the energy metabolism properties	243:274	the energy metabolism properties of skeletal muscle	243:293	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	4	54	theme	10	738:739	arg1	%					740:740	%	740:740	%	740:740	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	9	55	theme	myosin	1411:1416	arg1	chain					1424:1428	myosin heavy chain	1411:1428	a myosin heavy chain shift	1409:1434	Moreover, a myosin heavy chain shift occurred from type IIb to IIx in KD.					
33125406	6	56	theme	protein	966:972	arg1	expression					974:983	protein expression	966:983	protein expression of metabolic enzymes and regulatory factors	966:1027	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	10	57	theme	muscle	1717:1722	arg1	shift					1704:1708	the myosin heavy chain shift	1681:1708	the myosin heavy chain shift of EDL muscle	1681:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	2	58	theme	ketogenic	305:313	arg1	effects					320:326	ketogenic diet effects	305:326	ketogenic diet effects on muscle metabolic characteristics	305:362	However, ketogenic diet effects on muscle metabolic characteristics are diverse and sometimes countervailing.					
33125406	1	59	theme	high-fat	171:178	arg1	diet					180:183	an extremely high-fat diet	158:183	an extremely high-fat diet with extremely low carbohydrates	158:216	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	1	59	theme	high-fat	171:178	arg1	diet					152:155	the ketogenic diet	138:155	the ketogenic diet	138:155	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	4	60	theme	90	729:730	arg1	%					731:731	%	731:731	%	731:731	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	6	61	theme	heavy	941:945	arg1	chain					947:951	myosin heavy chain	934:951	the myosin heavy chain composition	930:963	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	5	62	theme	tetanic	885:891	arg1	force					893:897	tetanic force	885:897	tetanic force	885:897	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	1	63	theme	low	200:202	arg1	carbohydrates					204:216	extremely low carbohydrates	190:216	extremely low carbohydrates	190:216	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	6	64	theme	citrate	1034:1040	arg1	synthase					1042:1049	citrate synthase	1034:1049	citrate synthase activity	1034:1058	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	10	65	theme	contractile	1600:1610	arg1	function					1612:1619	muscle contractile function	1593:1619	muscle contractile function in sedentary male rats	1593:1642	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	8	66	theme	succinate	1261:1269	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	66	theme	succinate	1261:1269	arg1	dehydrogenase					1271:1283	succinate dehydrogenase	1261:1283	succinate dehydrogenase	1261:1283	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	4	67	theme	80	689:690	arg1	%					691:691	%	691:691	%	691:691	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	68	theme	0	751:751	arg1	%					752:752	%	752:752	%	752:752	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	6	69	theme	regulatory	1010:1019	arg1	factors					1021:1027	regulatory factors	1010:1027	regulatory factors	1010:1027	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	4	70	theme	control	653:659	arg1	diet					661:664	a control diet	651:664	a control diet (10% fat, 10% protein, 80% carbohydrate)	651:705	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	0	71	theme	digitorum	72:80	arg1	longus					82:87	extensor digitorum longus	63:87	extensor digitorum longus muscle of sedentary male rats	63:117	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	10	72	theme	male	1634:1637	arg1	rats					1639:1642	sedentary male rats	1624:1642	sedentary male rats	1624:1642	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	8	73	theme	c	1297:1297	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	73	theme	c	1297:1297	arg1	subunit					1307:1313	cytochrome c oxidase subunit 4	1286:1315	cytochrome c oxidase subunit 4	1286:1315	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	7	74	theme	muscle	1147:1152	arg1	fatigue					1154:1160	muscle fatigue	1147:1160	muscle fatigue	1147:1160	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	1	75	theme	diet	152:155	arg1	studies					127:133	Recent studies	120:133	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates,	120:217	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	0	76	theme	sedentary	99:107	arg1	rats					114:117	sedentary male rats	99:117	sedentary male rats	99:117	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	4	77	theme	male	492:495	arg1	rats					504:507	male Wistar rats	492:507	male Wistar rats (8 weeks of age)	492:524	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	0	78	theme	rats	114:117	arg1	muscle					89:94	extensor digitorum longus muscle	63:94	extensor digitorum longus muscle of sedentary male rats	63:117	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	7	79	located	found	1091:1095	arg1	fatigue					1154:1160	muscle fatigue	1147:1160	muscle fatigue	1147:1160	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	7	79	located	found	1091:1095	arg2	difference					1076:1085	No significant difference	1061:1085	No significant difference	1061:1085	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	7	79	located	found	1091:1095	arg1	forces					1137:1142	twitch or tetanic forces	1119:1142	twitch or tetanic forces	1119:1142	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	5	80	theme	extensor	806:813	arg1	EDL					833:835	EDL	833:835	EDL	833:835	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	5	80	theme	extensor	806:813	arg1	longus					825:830	extensor digitorum longus	806:830	the extensor digitorum longus (EDL) muscle	802:843	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	7	81	theme	tetanic	1129:1135	arg1	forces					1137:1142	twitch or tetanic forces	1119:1142	twitch or tetanic forces	1119:1142	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	8	82	theme	3-hydroxyacyl-CoA	1322:1338	arg1	activity					1196:1203	the KD citrate synthase activity	1172:1203	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	8	82	theme	3-hydroxyacyl-CoA	1322:1338	arg1	dehydrogenase					1340:1352	3-hydroxyacyl-CoA dehydrogenase	1322:1352	3-hydroxyacyl-CoA dehydrogenase	1322:1352	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	4	83	theme	%	678:678	arg1	fat					671:673	10% fat	667:673	10% fat	667:673	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	83	theme	%	678:678	arg1	protein					680:686	10% protein	676:686	10% protein	676:686	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	3	84	theme	muscle	454:459	arg1	performance					461:471	skeletal muscle performance	445:471	skeletal muscle performance	445:471	Furthermore, ketogenic diet effects on skeletal muscle performance are unknown.					
33125406	0	85	theme	diet	10:13	arg1	feeding					15:21	Ketogenic diet feeding	0:21	Ketogenic diet feeding	0:21	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	4	86	dep	diet	661:664	arg1	fat					671:673	10% fat	667:673	10% fat	667:673	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	86	dep	diet	661:664	arg1	carbohydrate					693:704	80% carbohydrate	689:704	80% carbohydrate	689:704	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	86	dep	diet	661:664	arg1	protein					680:686	10% protein	676:686	10% protein	676:686	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	10	87	from	function	1612:1619	arg1	rats					1639:1642	sedentary male rats	1624:1642	sedentary male rats	1624:1642	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	7	88	theme	twitch	1119:1124	arg1	forces					1137:1142	twitch or tetanic forces	1119:1142	twitch or tetanic forces	1119:1142	No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue.					
33125406	5	89	theme	feeding	786:792	arg1	period					794:799	the 4-week feeding period	775:799	the 4-week feeding period	775:799	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	4	90	theme	control	554:560	arg1	CON					569:571	CON	569:571	CON	569:571	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	90	theme	control	554:560	arg1	group					562:566	a control group	552:566	a control group (CON)	552:572	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	10	91	theme	Sema3A	1671:1676	arg1	involvement					1656:1666	involvement	1656:1666	involvement of Sema3A in the myosin heavy chain shift of EDL muscle	1656:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	4	92	theme	%	669:669	arg1	fat					671:673	10% fat	667:673	10% fat	667:673	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	92	theme	%	669:669	arg1	carbohydrate					693:704	80% carbohydrate	689:704	80% carbohydrate	689:704	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	92	theme	%	669:669	arg1	protein					680:686	10% protein	676:686	10% protein	676:686	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	0	93	theme	metabolism	40:49	arg1	property					51:58	aerobic metabolism property	32:58	aerobic metabolism property	32:58	Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats.					
33125406	1	94	theme	muscle	288:293	arg1	properties					265:274	the energy metabolism properties	243:274	the energy metabolism properties of skeletal muscle	243:293	Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle.					
33125406	3	95	theme	diet	429:432	arg1	effects					434:440	ketogenic diet effects	419:440	ketogenic diet effects on skeletal muscle performance	419:471	Furthermore, ketogenic diet effects on skeletal muscle performance are unknown.					
33125406	8	96	theme	citrate	1179:1185	arg1	synthase					1187:1194	the KD citrate synthase	1172:1194	the KD citrate synthase activity	1172:1203	However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON.					
33125406	6	97	theme	myosin	934:939	arg1	chain					947:951	myosin heavy chain	934:951	the myosin heavy chain composition	930:963	We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity.					
33125406	10	98	theme	heavy	1692:1696	arg1	chain					1698:1702	the myosin heavy chain	1681:1702	the myosin heavy chain shift of EDL muscle	1681:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	5	99	theme	longus	825:830	arg1	muscle					838:843	the extensor digitorum longus (EDL) muscle	802:843	the extensor digitorum longus (EDL) muscle	802:843	After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue.					
33125406	3	100	from	effects	434:440	arg1	performance					461:471	skeletal muscle performance	445:471	skeletal muscle performance	445:471	Furthermore, ketogenic diet effects on skeletal muscle performance are unknown.					
33125406	2	101	theme	diet	315:318	arg1	effects					320:326	ketogenic diet effects	305:326	ketogenic diet effects on muscle metabolic characteristics	305:362	However, ketogenic diet effects on muscle metabolic characteristics are diverse and sometimes countervailing.					
33125406	10	102	from	involvement	1656:1666	arg1	shift					1704:1708	the myosin heavy chain shift	1681:1708	the myosin heavy chain shift of EDL muscle	1681:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	9	103	theme	chain	1424:1428	arg1	shift					1430:1434	a myosin heavy chain shift	1409:1434	a myosin heavy chain shift	1409:1434	Moreover, a myosin heavy chain shift occurred from type IIb to IIx in KD.					
33125406	4	104	theme	diet	590:593	arg1	KD					602:603	KD	602:603	KD	602:603	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	104	theme	diet	590:593	arg1	group					595:599	a ketogenic diet group	578:599	a ketogenic diet group (KD)	578:604	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	10	105	theme	EDL	1713:1715	arg1	muscle					1717:1722	EDL muscle	1713:1722	EDL muscle	1713:1722	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
33125406	4	106	theme	%	691:691	arg1	carbohydrate					693:704	80% carbohydrate	689:704	80% carbohydrate	689:704	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	4	106	theme	%	691:691	arg1	fat					671:673	10% fat	667:673	10% fat	667:673	After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate).					
33125406	10	107	theme	4-week	1509:1514	arg1	diet					1526:1529	the 4-week ketogenic diet	1505:1529	the 4-week ketogenic diet	1505:1529	These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.					
32702424	10	0	theme	MTZ	1497:1499	arg1	adsorption					1483:1492	the adsorption	1479:1492	the adsorption of MTZ by CTS-MNPs	1479:1511	The adsorption thermodynamic revealed an endothermic and spontaneous reaction for the adsorption of MTZ by CTS-MNPs.					
32702424	6	1	theme	removal	898:904	arg1	%					921:921	100%	918:921	100%	918:921	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	1	theme	removal	898:904	arg1	efficiency					906:915	the maximum removal efficiency	886:915	the maximum removal efficiency (100%)	886:922	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	7	2	theme	model	1158:1162	arg1	predictions					1164:1174	model predictions	1158:1174	model predictions	1158:1174	It is found that the experimental findings for the response are in good agreement with model predictions.					
32702424	9	3	theme	MTZ	1363:1365	arg1	adsorption					1367:1376	the MTZ adsorption	1359:1376	the MTZ adsorption onto the CTS-MNPs	1359:1394	Also, pseudo-second-order model indicated more appropriate for describing the MTZ adsorption onto the CTS-MNPs.					
32702424	5	4	theme	P-value	745:751	arg1	lack					726:729	insignificant lack	712:729	insignificant lack of fit, lower P-value and also higher R2	712:770	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	6	5	theme	maximum	890:896	arg1	%					921:921	100%	918:921	100%	918:921	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	5	theme	maximum	890:896	arg1	efficiency					906:915	the maximum removal efficiency	886:915	the maximum removal efficiency (100%)	886:922	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	10	6	theme	adsorption	1401:1410	arg1	thermodynamic					1412:1424	The adsorption thermodynamic	1397:1424	The adsorption thermodynamic	1397:1424	The adsorption thermodynamic revealed an endothermic and spontaneous reaction for the adsorption of MTZ by CTS-MNPs.					
32702424	3	7	theme	magnetic	480:487	arg1	properties					489:498	magnetic properties	480:498	magnetic properties	480:498	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	7	theme	magnetic	480:487	arg1	morphologies					431:442	the surface morphologies	419:442	the surface morphologies	419:442	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	10	8	theme	endothermic	1438:1448	arg1	reaction					1466:1473	an endothermic and spontaneous reaction	1435:1473	an endothermic and spontaneous reaction for the adsorption of MTZ by CTS-MNPs	1435:1511	The adsorption thermodynamic revealed an endothermic and spontaneous reaction for the adsorption of MTZ by CTS-MNPs.					
32702424	4	9	theme	R	544:544	arg1	software					546:553	R software	544:553	R software using response surface methodology	544:588	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	3	10	theme	TEM	361:363	arg1	analyzes					384:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	8	11	dep	involves	1226:1233	arg1	Freundlich					1257:1266	Freundlich	1257:1266	Freundlich	1257:1266	The results declare MTZ adsorption onto CTS-MNPs involves a multilayer process (Freundlich isotherm model).					
32702424	0	12	dep	kinetic	116:122	arg1	thermodynamic					125:137	thermodynamic	125:137	thermodynamic	125:137	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	6	13	theme	CTS-MNPs	989:996	arg1	dosage					998:1003	CTS-MNPs dosage	989:1003	CTS-MNPs dosage	989:1003	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	4	14	theme	response	561:568	arg1	methodology					578:588	response surface methodology	561:588	response surface methodology	561:588	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	5	15	theme	insignificant	712:724	arg1	lack					726:729	insignificant lack	712:729	insignificant lack of fit, lower P-value and also higher R2	712:770	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	6	16	dep	pH	982:983	arg1	10 mg L-1					1059:1067	10 mg L-1	1059:1067	10 mg L-1	1059:1067	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	16	dep	pH	982:983	arg1	dosage					998:1003	CTS-MNPs dosage	989:1003	CTS-MNPs dosage	989:1003	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	16	dep	pH	982:983	arg1	90 min					1029:1034	90 min	1029:1034	90 min	1029:1034	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	16	dep	pH	982:983	arg1	2 g L-1					1006:1012	2 g L-1	1006:1012	2 g L-1	1006:1012	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	16	dep	pH	982:983	arg1	3					986:986	3	986:986	3	986:986	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	16	dep	pH	982:983	arg1	concentration					1044:1056	MTZ concentration	1040:1056	MTZ concentration	1040:1056	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	6	16	dep	pH	982:983	arg1	time					1023:1026	contact time	1015:1026	contact time	1015:1026	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	8	17	theme	isotherm	1268:1275	arg1	model					1277:1281	isotherm model	1268:1281	Freundlich isotherm model	1257:1281	The results declare MTZ adsorption onto CTS-MNPs involves a multilayer process (Freundlich isotherm model).					
32702424	3	18	theme	SEM	356:358	arg1	analyzes					384:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	1	19	theme	adsorbents	183:192	arg1	method					243:248	an effective method	230:248	an effective method of removing metronidazole (MTZ)	230:280	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	1	19	theme	adsorbents	183:192	arg1	development					168:178	The development	164:178	The development of adsorbents with high adsorption performance	164:225	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	3	20	theme	VSM	372:374	arg1	analyzes					384:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	6	21	theme	optimal	850:856	arg1	conditions					858:867	The predicted optimal conditions	836:867	The predicted optimal conditions with considering the maximum removal efficiency (100%)	836:922	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	7	22	theme	good	1138:1141	arg1	agreement					1143:1151	good agreement	1138:1151	good agreement with model predictions	1138:1174	It is found that the experimental findings for the response are in good agreement with model predictions.					
32702424	5	23	theme	fit	734:736	arg1	P-value					745:751	fit, lower P-value	734:751	fit, lower P-value	734:751	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	4	24	theme	factors	649:655	arg1	effect					633:638	the composition effect	617:638	the composition effect of input factors and output response	617:675	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	5	25	dep	fit	734:736	arg1	lower					739:743	lower	739:743	lower	739:743	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	6	26	theme	second-order	944:955	arg1	model					957:961	second-order model	944:961	second-order model	944:961	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	1	27	theme	high	199:202	arg1	performance					215:225	high adsorption performance	199:225	high adsorption performance	199:225	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	3	28	theme	FTIR	366:369	arg1	analyzes					384:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	6	29	theme	contact	1015:1021	arg1	time					1023:1026	contact time	1015:1026	contact time	1015:1026	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	10	30	theme	spontaneous	1454:1464	arg1	reaction					1466:1473	an endothermic and spontaneous reaction	1435:1473	an endothermic and spontaneous reaction for the adsorption of MTZ by CTS-MNPs	1435:1511	The adsorption thermodynamic revealed an endothermic and spontaneous reaction for the adsorption of MTZ by CTS-MNPs.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	properties					489:498	magnetic properties	480:498	magnetic properties	480:498	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	CTS-MNPs					520:527	CTS-MNPs	520:527	CTS-MNPs	520:527	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	size					455:458	size	455:458	size	455:458	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	size					501:504	size	501:504	size	501:504	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	groups					472:477	functional groups	461:477	functional groups	461:477	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	morphologies					431:442	the surface morphologies	419:442	the surface morphologies	419:442	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	volume					510:515	volume	510:515	volume of CTS-MNPs, respectively	510:541	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	31	theme	CTS-MNPs	520:527	arg1	shape					445:449	shape	445:449	shape	445:449	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	8	32	dep	Freundlich	1257:1266	arg1	model					1277:1281	isotherm model	1268:1281	Freundlich isotherm model	1257:1281	The results declare MTZ adsorption onto CTS-MNPs involves a multilayer process (Freundlich isotherm model).					
32702424	1	33	theme	adsorption	204:213	arg1	performance					215:225	high adsorption performance	199:225	high adsorption performance	199:225	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	5	34	theme	second-order	682:693	arg1	model					695:699	The second-order model	678:699	The second-order model because of insignificant lack of fit, lower P-value and also higher R2	678:770	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	0	35	from	wastewater	90:99	arg1	adsorption					57:66	the adsorption	53:66	the adsorption of metronidazole from wastewater	53:99	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	4	36	theme	response	668:675	arg1	effect					633:638	the composition effect	617:638	the composition effect of input factors and output response	617:675	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	6	37	theme	MTZ	1040:1042	arg1	concentration					1044:1056	MTZ concentration	1040:1056	MTZ concentration	1040:1056	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	0	38	theme	equilibrium	143:153	arg1	studies					155:161	equilibrium studies	143:161	equilibrium studies	143:161	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	5	39	theme	MTZ	819:821	arg1	adsorption					805:814	adsorption	805:814	adsorption of MTZ by CTS-MNPs	805:833	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	0	40	theme	kinetic	116:122	arg1	Optimization					102:113	Optimization	102:113	Optimization	102:113	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	4	41	theme	input	643:647	arg1	factors					649:655	input factors	643:655	input factors	643:655	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	4	42	theme	composition	621:631	arg1	effect					633:638	the composition effect	617:638	the composition effect of input factors and output response	617:675	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	0	43	theme	nano-adsorbent	34:47	arg1	Application					0:10	Application	0:10	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.	0:162	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	0	44	theme	Fe3O4-chitosan	19:32	arg1	nano-adsorbent					34:47	the Fe3O4-chitosan nano-adsorbent	15:47	the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater	15:99	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	8	45	theme	MTZ	1197:1199	arg1	adsorption					1201:1210	MTZ adsorption	1197:1210	MTZ adsorption onto CTS-MNPs	1197:1224	The results declare MTZ adsorption onto CTS-MNPs involves a multilayer process (Freundlich isotherm model).					
32702424	3	46	theme	BET	380:382	arg1	analyzes					384:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes	352:391	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	6	47	theme	predicted	840:848	arg1	conditions					858:867	The predicted optimal conditions	836:867	The predicted optimal conditions with considering the maximum removal efficiency (100%)	836:922	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	3	48	theme	functional	461:470	arg1	groups					472:477	functional groups	461:477	functional groups	461:477	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	48	theme	functional	461:470	arg1	morphologies					431:442	the surface morphologies	419:442	the surface morphologies	419:442	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	48	theme	functional	461:470	arg1	CTS-MNPs					520:527	CTS-MNPs	520:527	CTS-MNPs	520:527	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	5	49	theme	R2	769:770	arg1	lack					726:729	insignificant lack	712:729	insignificant lack of fit, lower P-value and also higher R2	712:770	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	9	50	theme	pseudo-second-order	1291:1309	arg1	model					1311:1315	pseudo-second-order model	1291:1315	pseudo-second-order model	1291:1315	Also, pseudo-second-order model indicated more appropriate for describing the MTZ adsorption onto the CTS-MNPs.					
32702424	6	51	dep	included	972:979	arg1	pH					982:983	pH	982:983	pH	982:983	The predicted optimal conditions with considering the maximum removal efficiency (100%) were calculated for second-order model and were included (pH, 3; CTS-MNPs dosage, 2 g L-1; contact time, 90 min and MTZ concentration, 10 mg L-1).					
32702424	1	52	theme	effective	233:241	arg1	method					243:248	an effective method	230:248	an effective method of removing metronidazole (MTZ)	230:280	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	1	52	theme	effective	233:241	arg1	development					168:178	The development	164:178	The development of adsorbents with high adsorption performance	164:225	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	1	53	with	adsorbents	183:192	arg1	performance					215:225	high adsorption performance	199:225	high adsorption performance	199:225	The development of adsorbents with high adsorption performance is an effective method of removing metronidazole (MTZ).					
32702424	2	54	theme	Fe3O4-chitosan	294:307	arg1	CTS-MNPs					325:332	CTS-MNPs	325:332	CTS-MNPs	325:332	Therefore, Fe3O4-chitosan nano-adsorbent (CTS-MNPs) was synthesized.					
32702424	2	54	theme	Fe3O4-chitosan	294:307	arg1	nano-adsorbent					309:322	Fe3O4-chitosan nano-adsorbent	294:322	Fe3O4-chitosan nano-adsorbent (CTS-MNPs)	294:333	Therefore, Fe3O4-chitosan nano-adsorbent (CTS-MNPs) was synthesized.					
32702424	8	55	theme	multilayer	1237:1246	arg1	process					1248:1254	a multilayer process	1235:1254	a multilayer process	1235:1254	The results declare MTZ adsorption onto CTS-MNPs involves a multilayer process (Freundlich isotherm model).					
32702424	4	56	theme	surface	570:576	arg1	methodology					578:588	response surface methodology	561:588	response surface methodology	561:588	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	0	57	theme	metronidazole	71:83	arg1	adsorption					57:66	the adsorption	53:66	the adsorption of metronidazole from wastewater	53:99	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	7	58	with	agreement	1143:1151	arg1	predictions					1164:1174	model predictions	1158:1174	model predictions	1158:1174	It is found that the experimental findings for the response are in good agreement with model predictions.					
32702424	4	59	theme	output	661:666	arg1	response					668:675	output response	661:675	output response	661:675	R software using response surface methodology was applied to investigate the composition effect of input factors and output response.					
32702424	3	60	theme	surface	423:429	arg1	properties					489:498	magnetic properties	480:498	magnetic properties	480:498	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	CTS-MNPs					520:527	CTS-MNPs	520:527	CTS-MNPs	520:527	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	size					455:458	size	455:458	size	455:458	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	size					501:504	size	501:504	size	501:504	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	groups					472:477	functional groups	461:477	functional groups	461:477	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	morphologies					431:442	the surface morphologies	419:442	the surface morphologies	419:442	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	volume					510:515	volume	510:515	volume of CTS-MNPs, respectively	510:541	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	3	60	theme	surface	423:429	arg1	shape					445:449	shape	445:449	shape	445:449	The SEM, TEM, FTIR, VSM and BET analyzes were applied to determine the surface morphologies, shape and size, functional groups, magnetic properties, size and volume of CTS-MNPs, respectively.					
32702424	5	61	theme	higher	762:767	arg1	R2					769:770	also higher R2	757:770	also higher R2	757:770	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32702424	0	62	dep	Application	0:10	arg1	Optimization					102:113	Optimization	102:113	Optimization	102:113	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	0	62	dep	Application	0:10	arg1	studies					155:161	equilibrium studies	143:161	equilibrium studies	143:161	Application of the Fe3O4-chitosan nano-adsorbent for the adsorption of metronidazole from wastewater: Optimization, kinetic, thermodynamic and equilibrium studies.					
32702424	7	63	theme	experimental	1092:1103	arg1	findings					1105:1112	the experimental findings	1088:1112	the experimental findings for the response	1088:1129	It is found that the experimental findings for the response are in good agreement with model predictions.					
32702424	5	64	theme	lack	726:729	arg1	model					695:699	The second-order model	678:699	The second-order model because of insignificant lack of fit, lower P-value and also higher R2	678:770	The second-order model because of insignificant lack of fit, lower P-value and also higher R2 indicated highly significant for adsorption of MTZ by CTS-MNPs.					
32171829	5	0	dep	adsorption	1087:1096	arg1	capacity					1098:1105	capacity	1098:1105	capacity	1098:1105	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	6	1	theme	LCK	1265:1267	arg1	composite					1276:1284	the LCK hybrid composite	1261:1284	the LCK hybrid composite	1261:1284	Adsorption-desorption experiments revealed that the LCK hybrid composite could be potentially reused with maintaining high adsorption efficiency.					
32171829	4	2	theme	adsorption	964:973	arg1	process					975:981	the adsorption process	960:981	the adsorption process	960:981	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	2	3	theme	present	377:383	arg1	work					385:388	The present work	373:388	The present work	373:388	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	5	4	theme	high	1060:1063	arg1	adsorption					1087:1096	extremely high nitrate and phosphate adsorption capacity and stability	1050:1119	adsorption	1087:1096	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	6	5	theme	adsorption	1336:1345	arg1	efficiency					1347:1356	high adsorption efficiency	1331:1356	high adsorption efficiency	1331:1356	Adsorption-desorption experiments revealed that the LCK hybrid composite could be potentially reused with maintaining high adsorption efficiency.					
32171829	4	6	theme	pseudo-second-order	776:794	arg1	model					804:808	pseudo-second-order kinetic model	776:808	pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments	776:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	2	7	theme	nitrate	563:569	arg1	remediation					548:558	the remediation	544:558	the remediation of nitrate and phosphate from water	544:594	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	4	8	theme	spontaneous	923:933	arg1	nature					950:955	spontaneous and exothermic nature	923:955	spontaneous and exothermic nature of the adsorption process	923:981	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	5	9	theme	hybrid	1023:1028	arg1	composite					1030:1038	the LCK hybrid composite	1015:1038	the LCK hybrid composite	1015:1038	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	1	10	theme	aquatic	305:311	arg1	environments					313:324	aquatic environments	305:324	aquatic environments	305:324	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	4	11	theme	LCK	751:753	arg1	composite					755:763	the LCK composite	747:763	the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments	747:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	1	12	theme	primary	178:184	arg1	phosphate					164:172	phosphate	164:172	phosphate	164:172	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	12	theme	primary	178:184	arg1	pollutants					186:195	primary pollutants	178:195	primary pollutants of water/wastewaters	178:216	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	12	theme	primary	178:184	arg1	Nitrate					152:158	Nitrate	152:158	Nitrate	152:158	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	0	13	theme	nitrate	118:124	arg1	recovery					106:113	the recovery	102:113	the recovery of nitrate and phosphate from water	102:149	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	4	14	theme	isotherm	871:878	arg1	model					880:884	Freundlich adsorption isotherm model	849:884	Freundlich adsorption isotherm model	849:884	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	0	15	theme	Adsorptive	0:9	arg1	performance					11:21	Adsorptive performance	0:21	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin	0:74	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	7	16	from	wastewater	1502:1511	arg1	denitrification					1452:1466	efficient denitrification	1442:1466	efficient denitrification	1442:1466	This study highlights the novel low-cost, eco-friendly and promising adsorbent for efficient denitrification and dephosphorization from water/ wastewater.					
32171829	7	16	from	wastewater	1502:1511	arg1	dephosphorization					1472:1488	dephosphorization	1472:1488	dephosphorization	1472:1488	This study highlights the novel low-cost, eco-friendly and promising adsorbent for efficient denitrification and dephosphorization from water/ wastewater.					
32171829	5	17	theme	electrostatic	1185:1197	arg1	interactions					1199:1210	electrostatic interactions	1185:1210	electrostatic interactions	1185:1210	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	4	18	theme	thermodynamic	890:902	arg1	experiments					904:914	thermodynamic experiments	890:914	thermodynamic experiments	890:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	3	19	theme	LCK	612:614	arg1	composite					623:631	The fabricated LCK hybrid composite	597:631	The fabricated LCK hybrid composite	597:631	The fabricated LCK hybrid composite was characterized using XRD, SEM, BET, EDAX, TGA-DTA and FTIR analysis.					
32171829	7	20	theme	efficient	1442:1450	arg1	denitrification					1452:1466	efficient denitrification	1442:1466	efficient denitrification	1442:1466	This study highlights the novel low-cost, eco-friendly and promising adsorbent for efficient denitrification and dephosphorization from water/ wastewater.					
32171829	2	21	theme	LCK	492:494	arg1	composite					504:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	1	22	theme	environments	313:324	arg1	security					293:300	the security	289:300	the security of aquatic environments	289:324	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	22	theme	environments	313:324	arg1	health					336:341	human health	330:341	human health	330:341	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	22	theme	environments	313:324	arg1	beings					365:370	all living beings	354:370	all living beings	354:370	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	3	23	theme	FTIR	690:693	arg1	analysis					695:702	FTIR analysis	690:702	FTIR analysis	690:702	The fabricated LCK hybrid composite was characterized using XRD, SEM, BET, EDAX, TGA-DTA and FTIR analysis.					
32171829	2	24	theme	adsorption	407:416	arg1	performance					418:428	adsorption performance	407:428	adsorption performance	407:428	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	0	25	theme	lanthanum	26:34	arg1	chitosan-kaolin					60:74	lanthanum encapsulated biopolymer chitosan-kaolin	26:74	lanthanum encapsulated biopolymer chitosan-kaolin	26:74	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	4	26	theme	process	975:981	arg1	nature					950:955	spontaneous and exothermic nature	923:955	spontaneous and exothermic nature of the adsorption process	923:981	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	6	27	theme	high	1331:1334	arg1	efficiency					1347:1356	high adsorption efficiency	1331:1356	high adsorption efficiency	1331:1356	Adsorption-desorption experiments revealed that the LCK hybrid composite could be potentially reused with maintaining high adsorption efficiency.					
32171829	2	28	theme	chitosan-kaolin	470:484	arg1	composite					504:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	2	29	theme	clay	486:489	arg1	composite					504:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	1	30	theme	human	330:334	arg1	health					336:341	human health	330:341	human health	330:341	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	0	31	theme	phosphate	130:138	arg1	recovery					106:113	the recovery	102:113	the recovery of nitrate and phosphate from water	102:149	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	7	32	dep	low-cost	1391:1398	arg1	adsorbent					1428:1436	adsorbent	1428:1436	adsorbent	1428:1436	This study highlights the novel low-cost, eco-friendly and promising adsorbent for efficient denitrification and dephosphorization from water/ wastewater.					
32171829	3	33	theme	fabricated	601:610	arg1	composite					623:631	The fabricated LCK hybrid composite	597:631	The fabricated LCK hybrid composite	597:631	The fabricated LCK hybrid composite was characterized using XRD, SEM, BET, EDAX, TGA-DTA and FTIR analysis.					
32171829	2	34	theme	encapsulated	457:468	arg1	composite					504:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	0	35	theme	biopolymer	49:58	arg1	chitosan-kaolin					60:74	lanthanum encapsulated biopolymer chitosan-kaolin	26:74	lanthanum encapsulated biopolymer chitosan-kaolin	26:74	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	6	36	theme	Adsorption-desorption	1213:1233	arg1	experiments					1235:1245	Adsorption-desorption experiments	1213:1245	Adsorption-desorption experiments	1213:1245	Adsorption-desorption experiments revealed that the LCK hybrid composite could be potentially reused with maintaining high adsorption efficiency.					
32171829	5	37	theme	phosphate	1077:1085	arg1	adsorption					1087:1096	extremely high nitrate and phosphate adsorption capacity and stability	1050:1119	adsorption	1087:1096	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	1	38	theme	water/wastewaters	200:216	arg1	phosphate					164:172	phosphate	164:172	phosphate	164:172	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	38	theme	water/wastewaters	200:216	arg1	pollutants					186:195	primary pollutants	178:195	primary pollutants of water/wastewaters	178:216	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	38	theme	water/wastewaters	200:216	arg1	Nitrate					152:158	Nitrate	152:158	Nitrate	152:158	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	2	39	theme	lanthanum	447:455	arg1	composite					504:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	0	40	theme	encapsulated	36:47	arg1	chitosan-kaolin					60:74	lanthanum encapsulated biopolymer chitosan-kaolin	26:74	lanthanum encapsulated biopolymer chitosan-kaolin	26:74	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	2	41	theme	phosphate	575:583	arg1	remediation					548:558	the remediation	544:558	the remediation of nitrate and phosphate from water	544:594	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	5	42	theme	ion	1154:1156	arg1	exchange					1158:1165	ion exchange	1154:1165	ion exchange	1154:1165	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	2	43	theme	composite	504:512	arg1	mechanism					434:442	mechanism	434:442	mechanism	434:442	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	2	43	theme	composite	504:512	arg1	performance					418:428	adsorption performance	407:428	adsorption performance	407:428	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	4	44	theme	adsorption	860:869	arg1	model					880:884	Freundlich adsorption isotherm model	849:884	Freundlich adsorption isotherm model	849:884	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	0	45	theme	chitosan-kaolin	60:74	arg1	performance					11:21	Adsorptive performance	0:21	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin	0:74	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	4	46	theme	exothermic	939:948	arg1	nature					950:955	spontaneous and exothermic nature	923:955	spontaneous and exothermic nature of the adsorption process	923:981	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	2	47	theme	hybrid	497:502	arg1	composite					504:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite	447:512	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	4	48	theme	Freundlich	849:858	arg1	model					880:884	Freundlich adsorption isotherm model	849:884	Freundlich adsorption isotherm model	849:884	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	4	49	theme	nitrate	720:726	arg1	removal					709:715	The removal	705:715	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments	705:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	2	50	used	utilized	531:538	arg2	performance					418:428	adsorption performance	407:428	adsorption performance	407:428	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	2	51	dep	performance	418:428	arg1	the					403:405	the	403:405	the	403:405	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
32171829	4	52	theme	kinetic	796:802	arg1	model					804:808	pseudo-second-order kinetic model	776:808	pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments	776:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	5	53	theme	LCK	1019:1021	arg1	composite					1030:1038	the LCK hybrid composite	1015:1038	the LCK hybrid composite	1015:1038	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	4	54	theme	phosphate	732:740	arg1	removal					709:715	The removal	705:715	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments	705:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	0	55	from	water	145:149	arg1	recovery					106:113	the recovery	102:113	the recovery of nitrate and phosphate from water	102:149	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	5	56	theme	nitrate	1065:1071	arg1	adsorption					1087:1096	extremely high nitrate and phosphate adsorption capacity and stability	1050:1119	adsorption	1087:1096	Results also demonstrated that the LCK hybrid composite exhibited extremely high nitrate and phosphate adsorption capacity and stability which followed the mechanisms by ion exchange, complexation and electrostatic interactions.					
32171829	6	57	theme	hybrid	1269:1274	arg1	composite					1276:1284	the LCK hybrid composite	1261:1284	the LCK hybrid composite	1261:1284	Adsorption-desorption experiments revealed that the LCK hybrid composite could be potentially reused with maintaining high adsorption efficiency.					
32171829	4	58	dep	model	804:808	arg1	described					836:844	described	836:844	are described by Freundlich adsorption isotherm model and thermodynamic experiments	832:914	The removal of nitrate and phosphate onto the LCK composite defined by pseudo-second-order kinetic model whereas the isotherms are described by Freundlich adsorption isotherm model and thermodynamic experiments showed spontaneous and exothermic nature of the adsorption process.					
32171829	0	59	theme	hybrid	81:86	arg1	composite					88:96	hybrid composite	81:96	hybrid composite	81:96	Adsorptive performance of lanthanum encapsulated biopolymer chitosan-kaolin clay hybrid composite for the recovery of nitrate and phosphate from water.					
32171829	1	60	theme	living	358:363	arg1	beings					365:370	all living beings	354:370	all living beings	354:370	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	1	61	dep	eutrophication	222:235	arg1	diseases					259:266	diseases	259:266	diseases	259:266	Nitrate and phosphate are primary pollutants of water/wastewaters for eutrophication and methemoglobinemia diseases, harshly threatening the security of aquatic environments and human health as well as all living beings.					
32171829	3	62	theme	hybrid	616:621	arg1	composite					623:631	The fabricated LCK hybrid composite	597:631	The fabricated LCK hybrid composite	597:631	The fabricated LCK hybrid composite was characterized using XRD, SEM, BET, EDAX, TGA-DTA and FTIR analysis.					
32171829	2	63	from	water	590:594	arg1	remediation					548:558	the remediation	544:558	the remediation of nitrate and phosphate from water	544:594	The present work investigates the adsorption performance and mechanism of lanthanum encapsulated chitosan-kaolin clay (LCK) hybrid composite was prepared and utilized for the remediation of nitrate and phosphate from water.					
34087707	6	0	theme	pork	1043:1046	arg1	TVB-N					1013:1017	TVB-N	1013:1017	TVB-N	1013:1017	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	0	theme	pork	1043:1046	arg1	change					1026:1031	pH change	1023:1031	pH change	1023:1031	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	0	theme	pork	1043:1046	arg1	change					1005:1010	the color change	995:1010	the color change	995:1010	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	1	1	theme	colorimetric	241:252	arg1	Hydrogels					173:181	Hydrogels	173:181	Hydrogels based on alginate and methylcellulose	173:219	Hydrogels based on alginate and methylcellulose were developed as a colorimetric indicator for monitoring minced pork spoilage.					
34087707	1	1	theme	colorimetric	241:252	arg1	indicator					254:262	a colorimetric indicator	239:262	a colorimetric indicator for monitoring minced pork spoilage	239:298	Hydrogels based on alginate and methylcellulose were developed as a colorimetric indicator for monitoring minced pork spoilage.					
34087707	0	2	theme	blend	71:75	arg1	hydrogel					77:84	an alginate-methylcellulose blend hydrogel	43:84	an alginate-methylcellulose blend hydrogel	43:84	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	4	3	theme	swelling	510:517	arg1	index					519:523	The hydrogel's swelling index	495:523	The hydrogel's swelling index	495:523	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	0	4	theme	indicator	162:170	arg1	development					124:134	the development	120:134	the development of a minced pork spoilage indicator	120:170	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	6	5	theme	minced	1036:1041	arg1	pork					1043:1046	minced pork	1036:1046	minced pork	1036:1046	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	5	6	theme	alginate	729:736	arg1	content					738:744	the alginate content	725:744	the alginate content	725:744	The hydrogel's compression strength is directly proportional to the alginate content.					
34087707	7	7	theme	intelligent	1143:1153	arg1	packaging					1155:1163	intelligent packaging	1143:1163	intelligent packaging	1143:1163	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	2	8	theme	crosslinking	378:389	arg1	agent					391:395	the crosslinking agent	374:395	the crosslinking agent	374:395	The hydrogel was fabricated by an external gelation method using Ca2+ as the crosslinking agent.					
34087707	2	8	theme	crosslinking	378:389	arg1	Ca2+					366:369	Ca2+	366:369	Ca2+	366:369	The hydrogel was fabricated by an external gelation method using Ca2+ as the crosslinking agent.					
34087707	0	9	theme	potential	94:102	arg1	opportunity					104:114	the potential opportunity	90:114	the potential opportunity for the development of a minced pork spoilage indicator	90:170	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	6	10	theme	total	843:847	arg1	TVB-N					874:878	TVB-N	874:878	TVB-N	874:878	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	10	theme	total	843:847	arg1	nitrogen					864:871	total volatile basic nitrogen	843:871	total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C	843:938	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	0	11	theme	ammonia	13:19	arg1	sensor					27:32	Colorimetric ammonia (NH3) sensor	0:32	Colorimetric ammonia (NH3) sensor	0:32	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	7	12	from	indicator	1130:1138	arg1	packaging					1155:1163	intelligent packaging	1143:1163	intelligent packaging	1143:1163	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	3	13	theme	blue	431:434	arg1	indicator					484:492	an indicator	481:492	an indicator	481:492	The pH-sensitive dye bromothymol blue was incorporated into the hydrogel to act as an indicator.					
34087707	3	13	theme	blue	431:434	arg1	dye					415:417	The pH-sensitive dye	398:417	The pH-sensitive dye bromothymol blue	398:434	The pH-sensitive dye bromothymol blue was incorporated into the hydrogel to act as an indicator.					
34087707	0	14	theme	Colorimetric	0:11	arg1	sensor					27:32	Colorimetric ammonia (NH3) sensor	0:32	Colorimetric ammonia (NH3) sensor	0:32	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	1	15	theme	minced	279:284	arg1	spoilage					291:298	minced pork spoilage	279:298	minced pork spoilage	279:298	Hydrogels based on alginate and methylcellulose were developed as a colorimetric indicator for monitoring minced pork spoilage.					
34087707	7	16	theme	potential	1078:1086	arg1	application					1088:1098	the potential application	1074:1098	the potential application of the hydrogel	1074:1114	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	7	16	theme	potential	1078:1086	arg1	indicator					1130:1138	a spoilage indicator	1119:1138	a spoilage indicator in intelligent packaging	1119:1163	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	0	17	theme	NH3	22:24	arg1	sensor					27:32	Colorimetric ammonia (NH3) sensor	0:32	Colorimetric ammonia (NH3) sensor	0:32	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	1	18	theme	pork	286:289	arg1	spoilage					291:298	minced pork spoilage	279:298	minced pork spoilage	279:298	Hydrogels based on alginate and methylcellulose were developed as a colorimetric indicator for monitoring minced pork spoilage.					
34087707	6	19	from	4 °C	935:938	arg1	storage					924:930	minced pork storage	912:930	minced pork storage at 4 °C	912:938	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	20	theme	color	785:789	arg1	change					791:796	a color change	783:796	a color change from orange to yellow (day 6)	783:826	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	4	21	theme	methylcellulose	563:577	arg1	ratio					554:558	an increasing ratio	540:558	an increasing ratio of methylcellulose	540:577	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	6	22	theme	basic	858:862	arg1	TVB-N					874:878	TVB-N	874:878	TVB-N	874:878	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	22	theme	basic	858:862	arg1	nitrogen					864:871	total volatile basic nitrogen	843:871	total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C	843:938	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	4	23	theme	polymer	640:646	arg1	composition					648:658	the polymer composition	636:658	the polymer composition	636:658	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	6	24	theme	pH	1023:1024	arg1	change					1026:1031	pH change	1023:1031	pH change	1023:1031	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	7	25	theme	hydrogel	1107:1114	arg1	application					1088:1098	the potential application	1074:1098	the potential application of the hydrogel	1074:1114	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	7	25	theme	hydrogel	1107:1114	arg1	indicator					1130:1138	a spoilage indicator	1119:1138	a spoilage indicator in intelligent packaging	1119:1163	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	6	26	theme	volatile	849:856	arg1	TVB-N					874:878	TVB-N	874:878	TVB-N	874:878	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	26	theme	volatile	849:856	arg1	nitrogen					864:871	total volatile basic nitrogen	843:871	total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C	843:938	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	27	theme	color	999:1003	arg1	change					1005:1010	the color change	995:1010	the color change	995:1010	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	6	28	theme	pork	919:922	arg1	storage					924:930	minced pork storage	912:930	minced pork storage at 4 °C	912:938	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	0	29	theme	minced	141:146	arg1	indicator					162:170	a minced pork spoilage indicator	139:170	a minced pork spoilage indicator	139:170	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	4	30	theme	increasing	543:552	arg1	ratio					554:558	an increasing ratio	540:558	an increasing ratio of methylcellulose	540:577	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	2	31	theme	gelation	344:351	arg1	method					353:358	an external gelation method	332:358	an external gelation method using Ca2+ as the crosslinking agent	332:395	The hydrogel was fabricated by an external gelation method using Ca2+ as the crosslinking agent.					
34087707	3	32	theme	pH-sensitive	402:413	arg1	indicator					484:492	an indicator	481:492	an indicator	481:492	The pH-sensitive dye bromothymol blue was incorporated into the hydrogel to act as an indicator.					
34087707	3	32	theme	pH-sensitive	402:413	arg1	dye					415:417	The pH-sensitive dye	398:417	The pH-sensitive dye bromothymol blue	398:434	The pH-sensitive dye bromothymol blue was incorporated into the hydrogel to act as an indicator.					
34087707	6	33	theme	minced	912:917	arg1	storage					924:930	minced pork storage	912:930	minced pork storage at 4 °C	912:938	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	5	34	theme	compression	676:686	arg1	strength					688:695	The hydrogel's compression strength	661:695	The hydrogel's compression strength	661:695	The hydrogel's compression strength is directly proportional to the alginate content.					
34087707	2	35	theme	external	335:342	arg1	method					353:358	an external gelation method	332:358	an external gelation method using Ca2+ as the crosslinking agent	332:395	The hydrogel was fabricated by an external gelation method using Ca2+ as the crosslinking agent.					
34087707	0	36	theme	spoilage	153:160	arg1	indicator					162:170	a minced pork spoilage indicator	139:170	a minced pork spoilage indicator	139:170	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	6	37	theme	hydrogel	751:758	arg1	indicator					760:768	The hydrogel indicator	747:768	The hydrogel indicator	747:768	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	4	38	theme	uptake	606:611	arg1	tunable					625:631	tunable	625:631	tunable	625:631	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	4	38	theme	uptake	606:611	arg1	capacity					613:620	the water uptake capacity	596:620	the water uptake capacity	596:620	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	0	39	theme	pork	148:151	arg1	indicator					162:170	a minced pork spoilage indicator	139:170	a minced pork spoilage indicator	139:170	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	4	40	theme	water	600:604	arg1	tunable					625:631	tunable	625:631	tunable	625:631	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	4	40	theme	water	600:604	arg1	capacity					613:620	the water uptake capacity	596:620	the water uptake capacity	596:620	The hydrogel's swelling index increased with an increasing ratio of methylcellulose, suggesting that the water uptake capacity is tunable by the polymer composition.					
34087707	0	41	theme	alginate-methylcellulose	46:69	arg1	hydrogel					77:84	an alginate-methylcellulose blend hydrogel	43:84	an alginate-methylcellulose blend hydrogel	43:84	Colorimetric ammonia (NH3) sensor based on an alginate-methylcellulose blend hydrogel and the potential opportunity for the development of a minced pork spoilage indicator.					
34087707	6	42	from	orange	803:808	arg1	change					791:796	a color change	783:796	a color change from orange to yellow (day 6)	783:826	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
34087707	7	43	theme	spoilage	1121:1128	arg1	application					1088:1098	the potential application	1074:1098	the potential application of the hydrogel	1074:1114	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	7	43	theme	spoilage	1121:1128	arg1	indicator					1130:1138	a spoilage indicator	1119:1138	a spoilage indicator in intelligent packaging	1119:1163	This result demonstrated the potential application of the hydrogel as a spoilage indicator in intelligent packaging.					
34087707	6	44	theme	positive	966:973	arg1	correlation					975:985	a positive correlation	964:985	a positive correlation between the color change, TVB-N and pH change of minced pork	964:1046	The hydrogel indicator demonstrated a color change from orange to yellow (day 6) upon detecting total volatile basic nitrogen (TVB-N) built up in the package during minced pork storage at 4 °C, and the results showed a positive correlation between the color change, TVB-N and pH change of minced pork.					
33104864	8	0	theme	uremic	1525:1530	arg1	toxins					1532:1537	uremic toxins	1525:1537	uremic toxins	1525:1537	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	2	1	theme	gut	350:352	arg1	microbiota					354:363	the gut microbiota	346:363	the gut microbiota	346:363	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	7	2	theme	intestinal	1405:1414	arg1	proteins					1436:1443	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	9	3	theme	SCFAs-producing	1795:1809	arg1	bacteria					1811:1818	SCFAs-producing bacteria	1795:1818	SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus)	1795:1855	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	8	4	theme	p-cresol	1580:1587	arg1	sulfate					1589:1595	p-cresol sulfate	1580:1595	p-cresol sulfate	1580:1595	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	8	4	theme	p-cresol	1580:1587	arg1	PCS					1575:1577	PCS	1575:1577	PCS (p-cresol sulfate)	1575:1596	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	7	5	dep	proteins	1436:1443	arg1	[ZO-1					1445:1449	[ZO-1	1445:1449	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	7	5	dep	proteins	1436:1443	arg1	occludens-1					1459:1469	zonula occludens-1	1452:1469	zonula occludens-1	1452:1469	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	4	6	theme	fatty	782:786	arg1	acid					788:791	short-chain fatty acid	770:791	short-chain fatty acid	770:791	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	6	theme	fatty	782:786	arg1	SCFA					764:767	SCFA	764:767	SCFA (short-chain fatty acid) concentrations	764:807	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	7	7	theme	intestinal	1339:1348	arg1	barrier					1361:1367	the intestinal epithelial barrier	1335:1367	the intestinal epithelial barrier	1335:1367	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	11	8	theme	hyperuricemia	2194:2206	arg1	treatment					2181:2189	the treatment	2177:2189	the treatment of hyperuricemia	2177:2206	CONCLUSIONS Our findings showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia.					
33104864	10	9	theme	inulin	1872:1877	arg1	treatment					1879:1887	inulin treatment	1872:1887	inulin treatment	1872:1887	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	6	10	theme	cytokines	1076:1084	arg1	higher					1135:1140	higher	1135:1140	higher	1135:1140	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	6	10	theme	cytokines	1076:1084	arg1	levels					1053:1058	the levels	1049:1058	the levels of inflammatory cytokines and the LPS (lipopolysaccharide)	1049:1117	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	4	11	theme	combat	582:587	arg1	hyperuricemia					589:601	combat hyperuricemia	582:601	combat hyperuricemia	582:601	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	6	12	theme	inulin	1246:1251	arg1	treatment					1253:1261	inulin treatment	1246:1261	inulin treatment	1246:1261	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	2	13	theme	inulin	293:298	arg1	role					285:288	the role	281:288	the role of inulin in hyperuricemia and microbial composition of the gut microbiota	281:363	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	1	14	theme	metabolic	196:204	arg1	function					206:213	metabolic function	196:213	metabolic function	196:213	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	5	15	theme	KO	1019:1020	arg1	mice					1022:1025	KO mice	1019:1025	KO mice	1019:1025	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	8	16	theme	KO	1630:1631	arg1	mice					1633:1636	inulin-treated KO mice	1615:1636	inulin-treated KO mice	1615:1636	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	6	17	theme	KO	1149:1150	arg1	group					1152:1156	the KO group	1145:1156	the KO group	1145:1156	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	4	18	theme	uric	656:659	arg1	levels					667:672	serum UA (uric acid) levels	646:672	serum UA (uric acid) levels	646:672	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	18	theme	uric	656:659	arg1	acid					661:664	uric acid	656:664	uric acid	656:664	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	6	19	theme	hyperuricemic	1222:1234	arg1	mice					1236:1239	hyperuricemic mice	1222:1239	hyperuricemic mice	1222:1239	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	5	20	theme	XOD	993:995	arg1	activity					973:980	activity	973:980	activity	973:980	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	5	20	theme	XOD	993:995	arg1	expression					958:967	expression	958:967	expression	958:967	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	10	21	link	microbiota-derived	1921:1938	arg1	SCFAs					1940:1944	gut microbiota-derived SCFAs	1917:1944	gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations)	1917:1994	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	10	22	theme	hyperuricemia	2066:2078	arg1	relief					2080:2085	hyperuricemia relief	2066:2085	hyperuricemia relief	2066:2085	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	10	23	theme	SCFAs	1940:1944	arg1	production					1903:1912	the production	1899:1912	the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice	1899:2005	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	5	24	theme	xanthine	998:1005	arg1	XOD					993:995	hepatic XOD	985:995	hepatic XOD (xanthine oxidase)	985:1014	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	5	24	theme	xanthine	998:1005	arg1	oxidase					1007:1013	xanthine oxidase	998:1013	xanthine oxidase	998:1013	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	4	25	from	alterations	747:757	arg1	mice					815:818	KO mice	812:818	KO mice	812:818	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	1	26	theme	dietary	140:146	arg1	fiber					148:152	fermentable dietary fiber	128:152	fermentable dietary fiber	128:152	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	10	27	theme	gut	1917:1919	arg1	SCFAs					1940:1944	gut microbiota-derived SCFAs	1917:1944	gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations)	1917:1994	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	9	28	theme	microbial	1707:1715	arg1	diversity					1717:1725	microbial diversity	1707:1725	microbial diversity	1707:1725	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	9	29	dep	Akkermansia	1827:1837	arg1	e.g.					1821:1824	e.g.	1821:1824	e.g.	1821:1824	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	5	30	theme	RESULTS	821:827	arg1	supplementation					836:850	RESULTS Inulin supplementation	821:850	RESULTS Inulin supplementation	821:850	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	7	31	theme	KO	1490:1491	arg1	mice					1493:1496	KO mice	1490:1496	KO mice	1490:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	4	32	theme	UA	652:653	arg1	levels					667:672	serum UA (uric acid) levels	646:672	serum UA (uric acid) levels	646:672	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	32	theme	UA	652:653	arg1	acid					661:664	uric acid	656:664	uric acid	656:664	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	12	33	theme	inulin	2251:2256	arg1	supplementation					2258:2272	inulin supplementation	2251:2272	inulin supplementation	2251:2272	Moreover, alleviation of hyperuricemia by inulin supplementation was, at least, partially conciliated by modulation of gut microbiota and its metabolites.					
33104864	4	34	from	integrity	719:727	arg1	mice					815:818	KO mice	812:818	KO mice	812:818	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	9	35	theme	Further	1639:1645	arg1	investigation					1647:1659	Further investigation	1639:1659	Further investigation	1639:1659	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	4	36	theme	epithelial	700:709	arg1	integrity					719:727	epithelial barrier integrity	700:727	epithelial barrier integrity	700:727	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	6	37	from	inflammation	1275:1286	arg1	mice					1294:1297	KO mice	1291:1297	KO mice	1291:1297	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	5	38	theme	ABCG2	921:925	arg1	expressions					906:916	the expressions	902:916	the expressions of ABCG2 in intestine	902:938	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	11	39	theme	promising	2141:2149	arg1	inulin					2125:2130	inulin	2125:2130	inulin	2125:2130	CONCLUSIONS Our findings showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia.					
33104864	11	39	theme	promising	2141:2149	arg1	candidate					2163:2171	a promising therapeutic candidate	2139:2171	a promising therapeutic candidate for the treatment of hyperuricemia	2139:2206	CONCLUSIONS Our findings showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia.					
33104864	2	40	theme	hyperuricemia	385:397	arg1	model					376:380	a mouse model	368:380	a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene	368:454	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	4	41	from	parameters	688:697	arg1	mice					815:818	KO mice	812:818	KO mice	812:818	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	2	42	theme	oxidase	442:448	arg1	gene					451:454	Uox (urate oxidase) gene	431:454	Uox (urate oxidase) gene	431:454	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	0	43	theme	Inulin	0:5	arg1	supplementation					7:21	Inulin supplementation	0:21	Inulin supplementation	0:21	Inulin supplementation ameliorates hyperuricemia and modulates gut microbiota in Uox-knockout mice.					
33104864	7	44	theme	zonula	1452:1457	arg1	[ZO-1					1445:1449	[ZO-1	1445:1449	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	7	44	theme	zonula	1452:1457	arg1	occludens-1					1459:1469	zonula occludens-1	1452:1469	zonula occludens-1	1452:1469	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	2	45	theme	gene	451:454	arg1	knockout					419:426	knockout	419:426	knockout	419:426	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	12	46	theme	microbiota	2332:2341	arg1	modulation					2314:2323	modulation	2314:2323	modulation of gut microbiota and its metabolites	2314:2361	Moreover, alleviation of hyperuricemia by inulin supplementation was, at least, partially conciliated by modulation of gut microbiota and its metabolites.					
33104864	0	47	theme	gut	63:65	arg1	microbiota					67:76	gut microbiota	63:76	gut microbiota in Uox-knockout mice	63:97	Inulin supplementation ameliorates hyperuricemia and modulates gut microbiota in Uox-knockout mice.					
33104864	5	48	theme	hepatic	985:991	arg1	XOD					993:995	hepatic XOD	985:995	hepatic XOD (xanthine oxidase)	985:1014	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	5	48	theme	hepatic	985:991	arg1	oxidase					1007:1013	xanthine oxidase	998:1013	xanthine oxidase	998:1013	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	9	49	theme	relative	1742:1749	arg1	abundance					1751:1759	the relative abundance	1738:1759	the relative abundance of beneficial bacteria	1738:1782	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	4	50	theme	fecal	730:734	arg1	alterations					747:757	fecal microbiota alterations	730:757	fecal microbiota alterations	730:757	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	3	51	theme	KO	465:466	arg1	Uox-knockout					469:480	METHODS KO (Uox-knockout) and WT (wild-type) mice	457:505	Uox-knockout	469:480	METHODS KO (Uox-knockout) and WT (wild-type) mice were given inulin or saline by gavage for 7 weeks.					
33104864	5	52	from	expressions	906:916	arg1	intestine					930:938	intestine	930:938	intestine	930:938	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	6	53	theme	LPS	1094:1096	arg1	higher					1135:1140	higher	1135:1140	higher	1135:1140	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	6	53	theme	LPS	1094:1096	arg1	levels					1053:1058	the levels	1049:1058	the levels of inflammatory cytokines and the LPS (lipopolysaccharide)	1049:1117	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	7	54	theme	tight	1420:1424	arg1	proteins					1436:1443	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	7	55	theme	TJ	1416:1417	arg1	proteins					1436:1443	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	9	56	theme	bacteria	1775:1782	arg1	abundance					1751:1759	the relative abundance	1738:1759	the relative abundance of beneficial bacteria	1738:1782	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	8	57	theme	serum	1509:1513	arg1	levels					1515:1520	serum levels	1509:1520	serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate),	1509:1597	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	6	58	theme	systemic	1197:1204	arg1	inflammation					1206:1217	systemic inflammation	1197:1217	systemic inflammation of hyperuricemic mice	1197:1239	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	2	59	theme	microbiota	354:363	arg1	hyperuricemia					303:315	hyperuricemia	303:315	hyperuricemia	303:315	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	2	59	theme	microbiota	354:363	arg1	composition					331:341	microbial composition	321:341	microbial composition	321:341	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	7	60	from	proteins	1436:1443	arg1	mice					1493:1496	KO mice	1490:1496	KO mice	1490:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	10	61	theme	KO	1999:2000	arg1	mice					2002:2005	KO mice	1999:2005	KO mice	1999:2005	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	4	62	from	changes	635:641	arg1	alterations					747:757	fecal microbiota alterations	730:757	fecal microbiota alterations	730:757	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	62	from	changes	635:641	arg1	acid					661:664	uric acid	656:664	uric acid	656:664	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	62	from	changes	635:641	arg1	integrity					719:727	epithelial barrier integrity	700:727	epithelial barrier integrity	700:727	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	62	from	changes	635:641	arg1	levels					667:672	serum UA (uric acid) levels	646:672	serum UA (uric acid) levels	646:672	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	62	from	changes	635:641	arg1	concentrations					794:807	SCFA (short-chain fatty acid) concentrations	764:807	SCFA (short-chain fatty acid) concentrations	764:807	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	62	from	changes	635:641	arg1	parameters					688:697	inflammatory parameters	675:697	inflammatory parameters	675:697	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	7	63	theme	increased	1385:1393	arg1	levels					1395:1400	increased levels	1385:1400	increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice	1385:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	4	64	theme	KO	812:813	arg1	mice					815:818	KO mice	812:818	KO mice	812:818	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	8	65	theme	toxins	1532:1537	arg1	levels					1515:1520	serum levels	1509:1520	serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate),	1509:1597	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	6	66	theme	WT	1176:1177	arg1	group					1179:1183	the WT group	1172:1183	the WT group	1172:1183	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	5	67	from	expression	958:967	arg1	mice					1022:1025	KO mice	1019:1025	KO mice	1019:1025	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	4	68	theme	short-chain	770:780	arg1	acid					788:791	short-chain fatty acid	770:791	short-chain fatty acid	770:791	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	68	theme	short-chain	770:780	arg1	SCFA					764:767	SCFA	764:767	SCFA (short-chain fatty acid) concentrations	764:807	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	7	69	theme	epithelial	1350:1359	arg1	barrier					1361:1367	the intestinal epithelial barrier	1335:1367	the intestinal epithelial barrier	1335:1367	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	7	70	from	levels	1395:1400	arg1	mice					1493:1496	KO mice	1490:1496	KO mice	1490:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	10	71	dep	acetate	1947:1953	arg1	concentrations					1980:1993	concentrations	1980:1993	concentrations	1980:1993	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	6	72	theme	inflammatory	1063:1074	arg1	cytokines					1076:1084	inflammatory cytokines	1063:1084	inflammatory cytokines	1063:1084	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	5	73	from	activity	973:980	arg1	mice					1022:1025	KO mice	1019:1025	KO mice	1019:1025	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	6	74	theme	mice	1236:1239	arg1	inflammation					1206:1217	systemic inflammation	1197:1217	systemic inflammation of hyperuricemic mice	1197:1239	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	8	75	theme	inulin-treated	1615:1628	arg1	mice					1633:1636	inulin-treated KO mice	1615:1636	inulin-treated KO mice	1615:1636	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	12	76	theme	hyperuricemia	2234:2246	arg1	alleviation					2219:2229	alleviation	2219:2229	alleviation of hyperuricemia by inulin supplementation	2219:2272	Moreover, alleviation of hyperuricemia by inulin supplementation was, at least, partially conciliated by modulation of gut microbiota and its metabolites.					
33104864	10	77	theme	relief	2080:2085	arg1	effectiveness					2049:2061	the effectiveness	2045:2061	the effectiveness of hyperuricemia relief	2045:2085	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	1	78	theme	fermentable	128:138	arg1	fiber					148:152	fermentable dietary fiber	128:152	fermentable dietary fiber	128:152	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	7	79	theme	inulin	1309:1314	arg1	treatment					1316:1324	inulin treatment	1309:1324	inulin treatment	1309:1324	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	10	80	dep	SCFAs	1940:1944	arg1	acetate					1947:1953	acetate	1947:1953	acetate	1947:1953	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	10	80	dep	SCFAs	1940:1944	arg1	butyrate					1971:1978	butyrate	1971:1978	butyrate	1971:1978	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	10	80	dep	SCFAs	1940:1944	arg1	propionate					1956:1965	propionate	1956:1965	propionate	1956:1965	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	10	81	theme	microbiota-derived	1921:1938	arg1	SCFAs					1940:1944	gut microbiota-derived SCFAs	1917:1944	gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations)	1917:1994	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	0	82	theme	Uox-knockout	81:92	arg1	mice					94:97	Uox-knockout mice	81:97	Uox-knockout mice	81:97	Inulin supplementation ameliorates hyperuricemia and modulates gut microbiota in Uox-knockout mice.					
33104864	1	83	theme	fiber	148:152	arg1	Inulin					108:113	PURPOSE Inulin	100:113	PURPOSE Inulin	100:113	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	1	83	theme	fiber	148:152	arg1	type					120:123	a type	118:123	a type	118:123	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	1	83	theme	fiber	148:152	arg1	non-digestible					164:177	non-digestible	164:177	non-digestible	164:177	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	2	84	from	role	285:288	arg1	hyperuricemia					303:315	hyperuricemia	303:315	hyperuricemia	303:315	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	2	84	from	role	285:288	arg1	composition					331:341	microbial composition	321:341	microbial composition	321:341	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	10	85	dep	increased	1889:1897	arg1	correlated					2029:2038	correlated	2029:2038	was positively correlated with the effectiveness of hyperuricemia relief	2014:2085	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	8	86	dep	toxins	1532:1537	arg1	including					1540:1548	including	1540:1548	including IS (indoxyl sulfate) and PCS (p-cresol sulfate)	1540:1596	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	5	87	theme	Inulin	829:834	arg1	supplementation					836:850	RESULTS Inulin supplementation	821:850	RESULTS Inulin supplementation	821:850	RESULTS Inulin supplementation can effectively alleviate hyperuricemia, increase the expressions of ABCG2 in intestine, and downregulate expression and activity of hepatic XOD (xanthine oxidase) in KO mice.					
33104864	7	88	theme	occluding	1476:1484	arg1	levels					1395:1400	increased levels	1385:1400	increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice	1385:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	9	89	theme	inulin	1675:1680	arg1	supplementation					1682:1696	inulin supplementation	1675:1696	inulin supplementation	1675:1696	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	4	90	theme	serum	646:650	arg1	levels					667:672	serum UA (uric acid) levels	646:672	serum UA (uric acid) levels	646:672	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	90	theme	serum	646:650	arg1	acid					661:664	uric acid	656:664	uric acid	656:664	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	6	91	theme	KO	1291:1292	arg1	mice					1294:1297	KO mice	1291:1297	KO mice	1291:1297	It was revealed that the levels of inflammatory cytokines and the LPS (lipopolysaccharide) were remarkably higher in the KO group than those in the WT group, indicating systemic inflammation of hyperuricemic mice, but inulin treatment ameliorated inflammation in KO mice.					
33104864	4	92	from	concentrations	794:807	arg1	mice					815:818	KO mice	812:818	KO mice	812:818	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	0	93	from	microbiota	67:76	arg1	mice					94:97	Uox-knockout mice	81:97	Uox-knockout mice	81:97	Inulin supplementation ameliorates hyperuricemia and modulates gut microbiota in Uox-knockout mice.					
33104864	11	94	dep	CONCLUSIONS	2088:2098	arg1	showed					2113:2118	showed	2113:2118	showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia	2113:2206	CONCLUSIONS Our findings showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia.					
33104864	11	95	theme	therapeutic	2151:2161	arg1	inulin					2125:2130	inulin	2125:2130	inulin	2125:2130	CONCLUSIONS Our findings showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia.					
33104864	11	95	theme	therapeutic	2151:2161	arg1	candidate					2163:2171	a promising therapeutic candidate	2139:2171	a promising therapeutic candidate for the treatment of hyperuricemia	2139:2206	CONCLUSIONS Our findings showed that inulin may be a promising therapeutic candidate for the treatment of hyperuricemia.					
33104864	4	96	theme	barrier	711:717	arg1	integrity					719:727	epithelial barrier integrity	700:727	epithelial barrier integrity	700:727	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	10	97	from	production	1903:1912	arg1	mice					2002:2005	KO mice	1999:2005	KO mice	1999:2005	Additionally, inulin treatment increased the production of gut microbiota-derived SCFAs (acetate, propionate and butyrate concentrations) in KO mice, which was positively correlated with the effectiveness of hyperuricemia relief.					
33104864	1	98	theme	PURPOSE	100:106	arg1	Inulin					108:113	PURPOSE Inulin	100:113	PURPOSE Inulin	100:113	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	1	98	theme	PURPOSE	100:106	arg1	type					120:123	a type	118:123	a type	118:123	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	1	98	theme	PURPOSE	100:106	arg1	non-digestible					164:177	non-digestible	164:177	non-digestible	164:177	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	2	99	theme	urate	436:440	arg1	gene					451:454	Uox (urate oxidase) gene	431:454	Uox (urate oxidase) gene	431:454	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	2	100	theme	mouse	370:374	arg1	model					376:380	a mouse model	368:380	a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene	368:454	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	4	101	from	levels	667:672	arg1	mice					815:818	KO mice	812:818	KO mice	812:818	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	2	102	theme	Uox	431:433	arg1	gene					451:454	Uox (urate oxidase) gene	431:454	Uox (urate oxidase) gene	431:454	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	4	103	theme	inflammatory	675:686	arg1	parameters					688:697	inflammatory parameters	675:697	inflammatory parameters	675:697	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	3	104	theme	METHODS	457:463	arg1	Uox-knockout					469:480	METHODS KO (Uox-knockout) and WT (wild-type) mice	457:505	Uox-knockout	469:480	METHODS KO (Uox-knockout) and WT (wild-type) mice were given inulin or saline by gavage for 7 weeks.					
33104864	7	105	from	occluding	1476:1484	arg1	mice					1493:1496	KO mice	1490:1496	KO mice	1490:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	12	106	theme	gut	2328:2330	arg1	microbiota					2332:2341	gut microbiota	2328:2341	gut microbiota	2328:2341	Moreover, alleviation of hyperuricemia by inulin supplementation was, at least, partially conciliated by modulation of gut microbiota and its metabolites.					
33104864	12	107	theme	metabolites	2351:2361	arg1	modulation					2314:2323	modulation	2314:2323	modulation of gut microbiota and its metabolites	2314:2361	Moreover, alleviation of hyperuricemia by inulin supplementation was, at least, partially conciliated by modulation of gut microbiota and its metabolites.					
33104864	4	108	theme	SCFA	764:767	arg1	concentrations					794:807	SCFA (short-chain fatty acid) concentrations	764:807	SCFA (short-chain fatty acid) concentrations	764:807	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	3	109	theme	WT	487:488	arg1	mice					502:505	METHODS KO (Uox-knockout) and WT (wild-type) mice	457:505	mice	502:505	METHODS KO (Uox-knockout) and WT (wild-type) mice were given inulin or saline by gavage for 7 weeks.					
33104864	7	110	from	mice	1493:1496	arg1	levels					1395:1400	increased levels	1385:1400	increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice	1385:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	7	111	theme	proteins	1436:1443	arg1	levels					1395:1400	increased levels	1385:1400	increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice	1385:1496	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	2	112	theme	microbial	321:329	arg1	composition					331:341	microbial composition	321:341	microbial composition	321:341	This study aimed to evaluate the role of inulin in hyperuricemia and microbial composition of the gut microbiota in a mouse model of hyperuricemia established through knockout of Uox (urate oxidase) gene.					
33104864	8	113	theme	indoxyl	1554:1560	arg1	IS					1550:1551	IS	1550:1551	IS	1550:1551	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	8	113	theme	indoxyl	1554:1560	arg1	sulfate					1562:1568	indoxyl sulfate	1554:1568	indoxyl sulfate	1554:1568	Moreover, serum levels of uremic toxins, including IS (indoxyl sulfate) and PCS (p-cresol sulfate), were reduced in inulin-treated KO mice.					
33104864	1	114	theme	intestinal	229:238	arg1	microbiota					240:249	intestinal microbiota	229:249	intestinal microbiota	229:249	PURPOSE Inulin is a type of fermentable dietary fiber, which is non-digestible, and can improve metabolic function by modulating intestinal microbiota.					
33104864	7	115	theme	junction	1426:1433	arg1	proteins					1436:1443	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1)	1405:1470	Besides, inulin treatment repaired the intestinal epithelial barrier as evidenced by increased levels of intestinal TJ (tight junction) proteins [ZO-1 (zonula occludens-1) and occluding] in KO mice.					
33104864	9	116	theme	beneficial	1764:1773	arg1	bacteria					1775:1782	beneficial bacteria	1764:1782	beneficial bacteria	1764:1782	Further investigation unveiled that inulin supplementation enhanced microbial diversity and raised the relative abundance of beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia and Ruminococcus).					
33104864	4	117	theme	inulin	572:577	arg1	effect					562:567	The effect	558:567	The effect of inulin to combat hyperuricemia	558:601	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	4	118	theme	microbiota	736:745	arg1	alterations					747:757	fecal microbiota alterations	730:757	fecal microbiota alterations	730:757	The effect of inulin to combat hyperuricemia was determined by assessing the changes in serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal microbiota alterations, and SCFA (short-chain fatty acid) concentrations in KO mice.					
33104864	3	119	theme	wild-type	491:499	arg1	mice					502:505	METHODS KO (Uox-knockout) and WT (wild-type) mice	457:505	mice	502:505	METHODS KO (Uox-knockout) and WT (wild-type) mice were given inulin or saline by gavage for 7 weeks.					
33430526	0	0	theme	Barley	74:79	arg1	Grain					81:85	Barley Grain	74:85	Barley Grain	74:85	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	0	1	from	Study	24:28	arg1	Starch					39:44	Total Starch	33:44	Total Starch	33:44	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	0	1	from	Study	24:28	arg1	Amylopectin					59:69	Amylopectin	59:69	Amylopectin	59:69	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	0	1	from	Study	24:28	arg1	Grain					81:85	Barley Grain	74:85	Barley Grain	74:85	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	0	1	from	Study	24:28	arg1	Amylose					47:53	Amylose	47:53	Amylose	47:53	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	5	2	theme	GWAS	555:558	arg1	analysis					560:567	GWAS analysis	555:567	GWAS analysis	555:567	GWAS analysis detected 13, 2, 10 QTLs for TS, AC and AP, respectively, and 5 of them were commonly shared by AP and TS content.					
33430526	5	3	theme	TS	671:672	arg1	content					674:680	TS content	671:680	TS content	671:680	GWAS analysis detected 13, 2, 10 QTLs for TS, AC and AP, respectively, and 5 of them were commonly shared by AP and TS content.					
33430526	2	4	dep	genes	260:264	arg1	the					256:258	the	256:258	the	256:258	Few studies have been done on the identification of the genes or loci associated with these traits in barley.					
33430526	6	5	theme	largest	728:734	arg1	variation					736:744	the largest variation	724:744	the largest variation of TS, AC and AP, respectively	724:775	qTS-3.1, qAC-6.2 and qAP-5.1 may explain the largest variation of TS, AC and AP, respectively.					
33430526	4	6	theme	large	488:492	arg1	variation					504:512	A large genotypic variation	486:512	A large genotypic variation	486:512	A large genotypic variation was found in all examined starch traits.					
33430526	6	7	theme	TS	749:750	arg1	variation					736:744	the largest variation	724:744	the largest variation of TS, AC and AP, respectively	724:775	qTS-3.1, qAC-6.2 and qAP-5.1 may explain the largest variation of TS, AC and AP, respectively.					
33430526	2	8	from	traits	296:301	arg1	barley					306:311	barley	306:311	barley	306:311	Few studies have been done on the identification of the genes or loci associated with these traits in barley.					
33430526	1	9	theme	starch	150:155	arg1	composition					135:145	composition	135:145	composition	135:145	The content and composition of starch in cereal grains are closely related to yield.					
33430526	1	9	theme	starch	150:155	arg1	content					123:129	content	123:129	content	123:129	The content and composition of starch in cereal grains are closely related to yield.					
33430526	10	10	from	loci	1344:1347	arg1	study					1366:1370	this study	1361:1370	this study	1361:1370	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	7	11	theme	barley	935:940	arg1	grains					942:947	the developing barley grains	920:947	the developing barley grains	920:947	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	12	theme	putative	783:790	arg1	HORVU6Hr1G087920					815:830	HORVU6Hr1G087920	815:830	HORVU6Hr1G087920	815:830	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	12	theme	putative	783:790	arg1	HORVU5Hr1G011280					872:887	HORVU5Hr1G011280	872:887	HORVU5Hr1G011280	872:887	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	12	theme	putative	783:790	arg1	genes					802:806	Four putative candidate genes	778:806	Four putative candidate genes	778:806	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	12	theme	putative	783:790	arg1	HORVU5Hr1G011270					851:866	HORVU5Hr1G011270	851:866	HORVU5Hr1G011270	851:866	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	12	theme	putative	783:790	arg1	HORVU5Hr1G011230					833:848	HORVU5Hr1G011230	833:848	HORVU5Hr1G011230	833:848	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	2	13	theme	genes	260:264	arg1	identification					238:251	the identification	234:251	the identification of the genes or loci associated with these traits in barley	234:311	Few studies have been done on the identification of the genes or loci associated with these traits in barley.					
33430526	9	14	theme	favorable	1249:1257	arg1	alleles					1259:1265	favorable alleles	1249:1265	favorable alleles of the identified QTLs and TS, AC, AP content	1249:1311	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	8	15	theme	examined	986:993	arg1	accessions					1006:1015	100 barley accessions	995:1015	The examined 100 barley accessions	982:1015	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	4	16	theme	examined	531:538	arg1	traits					547:552	all examined starch traits	527:552	all examined starch traits	527:552	A large genotypic variation was found in all examined starch traits.					
33430526	1	17	dep	content	123:129	arg1	The					119:121	The	119:121	The	119:121	The content and composition of starch in cereal grains are closely related to yield.					
33430526	4	18	theme	genotypic	494:502	arg1	variation					504:512	A large genotypic variation	486:512	A large genotypic variation	486:512	A large genotypic variation was found in all examined starch traits.					
33430526	0	19	theme	Putative	101:108	arg1	Alleles					110:116	Novel Putative Alleles	95:116	Novel Putative Alleles	95:116	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	3	20	dep	genes	355:359	arg1	the					351:353	the	351:353	the	351:353	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	1	21	from	composition	135:145	arg1	grains					167:172	cereal grains	160:172	cereal grains	160:172	The content and composition of starch in cereal grains are closely related to yield.					
33430526	8	22	theme	S5H_29297679	1090:1101	arg1	polymorphism					1063:1074	the polymorphism	1059:1074	the polymorphism of the marker S5H_29297679	1059:1101	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	9	23	theme	identified	1274:1283	arg1	QTLs					1285:1288	the identified QTLs	1270:1288	the identified QTLs	1270:1288	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	0	24	theme	Novel	95:99	arg1	Alleles					110:116	Novel Putative Alleles	95:116	Novel Putative Alleles	95:116	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	10	25	from	starch	1422:1427	arg1	barley					1432:1437	barley	1432:1437	barley	1432:1437	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	7	26	theme	developing	924:933	arg1	grains					942:947	the developing barley grains	920:947	the developing barley grains	920:947	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	1	27	theme	cereal	160:165	arg1	grains					167:172	cereal grains	160:172	cereal grains	160:172	The content and composition of starch in cereal grains are closely related to yield.					
33430526	0	28	theme	Association	12:22	arg1	Study					24:28	Genome-Wide Association Study	0:28	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain	0:85	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	10	29	theme	identified	1333:1342	arg1	loci					1344:1347	loci	1344:1347	loci	1344:1347	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	10	29	theme	identified	1333:1342	arg1	useful					1381:1386	useful	1381:1386	useful	1381:1386	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	1	30	from	content	123:129	arg1	grains					167:172	cereal grains	160:172	cereal grains	160:172	The content and composition of starch in cereal grains are closely related to yield.					
33430526	3	31	theme	starch	381:386	arg1	traits					388:393	starch traits	381:393	starch traits in barley grains	381:410	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	0	32	theme	Genome-Wide	0:10	arg1	Association					12:22	Genome-Wide Association	0:22	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain	0:85	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	7	33	from	expression	906:915	arg1	grains					942:947	the developing barley grains	920:947	the developing barley grains	920:947	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	8	34	theme	marker	1083:1088	arg1	S5H_29297679					1090:1101	the marker S5H_29297679	1079:1101	the marker S5H_29297679	1079:1101	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	9	35	theme	QTLs	1285:1288	arg1	alleles					1259:1265	favorable alleles	1249:1265	favorable alleles of the identified QTLs and TS, AC, AP content	1249:1311	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	2	36	theme	loci	269:272	arg1	identification					238:251	the identification	234:251	the identification of the genes or loci associated with these traits in barley	234:311	Few studies have been done on the identification of the genes or loci associated with these traits in barley.					
33430526	4	37	theme	starch	540:545	arg1	traits					547:552	all examined starch traits	527:552	all examined starch traits	527:552	A large genotypic variation was found in all examined starch traits.					
33430526	7	38	theme	high	901:904	arg1	expression					906:915	the high expression	897:915	the high expression in the developing barley grains	897:947	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	8	39	contain	having	1161:1166	arg2	AA					1168:1169	AA	1168:1169	AA	1168:1169	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	8	39	contain	having	1161:1166	arg1	accessions					1150:1159	seven accessions	1144:1159	seven accessions having AA	1144:1169	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	9	40	theme	alleles	1259:1265	arg1	number					1239:1244	the number	1235:1244	the number of favorable alleles of the identified QTLs and TS, AC, AP content	1235:1311	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	3	41	theme	total	423:427	arg1	TS					437:438	TS	437:438	TS	437:438	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	3	41	theme	total	423:427	arg1	starch					429:434	total starch	423:434	total starch (TS)	423:439	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	10	42	theme	starch	1422:1427	arg1	grains					1415:1420	grains	1415:1420	grains starch in barley	1415:1437	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	0	43	theme	Total	33:37	arg1	Starch					39:44	Total Starch	33:44	Total Starch	33:44	Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.					
33430526	5	44	theme	them	635:638	arg1	them					635:638	them	635:638	them	635:638	GWAS analysis detected 13, 2, 10 QTLs for TS, AC and AP, respectively, and 5 of them were commonly shared by AP and TS content.					
33430526	5	44	theme	them	635:638	arg1	5					630:630	5	630:630	5	630:630	GWAS analysis detected 13, 2, 10 QTLs for TS, AC and AP, respectively, and 5 of them were commonly shared by AP and TS content.					
33430526	10	45	from	genes	1352:1356	arg1	study					1366:1370	this study	1361:1370	this study	1361:1370	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	4	46	located	found	518:522	arg1	traits					547:552	all examined starch traits	527:552	all examined starch traits	527:552	A large genotypic variation was found in all examined starch traits.					
33430526	4	46	located	found	518:522	arg2	variation					504:512	A large genotypic variation	486:512	A large genotypic variation	486:512	A large genotypic variation was found in all examined starch traits.					
33430526	10	47	from	barley	1432:1437	arg1	starch					1422:1427	starch	1422:1427	starch	1422:1427	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	8	48	theme	barley	999:1004	arg1	accessions					1006:1015	100 barley accessions	995:1015	The examined 100 barley accessions	982:1015	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	6	49	theme	AP	760:761	arg1	variation					736:744	the largest variation	724:744	the largest variation of TS, AC and AP, respectively	724:775	qTS-3.1, qAC-6.2 and qAP-5.1 may explain the largest variation of TS, AC and AP, respectively.					
33430526	8	50	contain	having	1123:1128	arg2	accessions					1150:1159	seven accessions	1144:1159	seven accessions having AA	1144:1169	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	8	50	contain	having	1123:1128	arg1	accessions					1112:1121	93 accessions	1109:1121	93 accessions having allele GG and seven accessions having AA	1109:1169	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	8	50	contain	having	1123:1128	arg2	GG					1137:1138	allele GG	1130:1138	allele GG	1130:1138	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	2	51	theme	Few	204:206	arg1	studies					208:214	Few studies	204:214	Few studies	204:214	Few studies have been done on the identification of the genes or loci associated with these traits in barley.					
33430526	9	52	theme	positive	1196:1203	arg1	correlation					1205:1215	significantly positive correlation	1182:1215	significantly positive correlation	1182:1215	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	9	53	theme	TS	1294:1295	arg1	alleles					1259:1265	favorable alleles	1249:1265	favorable alleles of the identified QTLs and TS, AC, AP content	1249:1311	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	9	54	theme	AP	1302:1303	arg1	content					1305:1311	AP content	1302:1311	AP content	1302:1311	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	9	54	theme	AP	1302:1303	arg1	TS					1294:1295	TS	1294:1295	TS	1294:1295	Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content.					
33430526	6	55	theme	AC	753:754	arg1	variation					736:744	the largest variation	724:744	the largest variation of TS, AC and AP, respectively	724:775	qTS-3.1, qAC-6.2 and qAP-5.1 may explain the largest variation of TS, AC and AP, respectively.					
33430526	10	56	from	conclusion	1317:1326	arg1	useful					1381:1386	useful	1381:1386	useful	1381:1386	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	10	56	from	conclusion	1317:1326	arg1	loci					1344:1347	loci	1344:1347	loci	1344:1347	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	8	57	theme	allele	1130:1135	arg1	GG					1137:1138	allele GG	1130:1138	allele GG	1130:1138	The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA.					
33430526	3	58	theme	AP	472:473	arg1	contents					476:483	amylopectin (AP) contents	459:483	amylopectin (AP) contents	459:483	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	3	59	theme	amylopectin	459:469	arg1	contents					476:483	amylopectin (AP) contents	459:483	amylopectin (AP) contents	459:483	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	3	60	from	traits	388:393	arg1	grains					405:410	barley grains	398:410	barley grains	398:410	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	3	61	theme	barley	398:403	arg1	grains					405:410	barley grains	398:410	barley grains	398:410	This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents.					
33430526	10	62	from	useful	1381:1386	arg1	conclusion					1317:1326	conclusion	1317:1326	conclusion	1317:1326	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	7	63	theme	candidate	792:800	arg1	HORVU6Hr1G087920					815:830	HORVU6Hr1G087920	815:830	HORVU6Hr1G087920	815:830	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	63	theme	candidate	792:800	arg1	HORVU5Hr1G011280					872:887	HORVU5Hr1G011280	872:887	HORVU5Hr1G011280	872:887	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	63	theme	candidate	792:800	arg1	genes					802:806	Four putative candidate genes	778:806	Four putative candidate genes	778:806	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	63	theme	candidate	792:800	arg1	HORVU5Hr1G011270					851:866	HORVU5Hr1G011270	851:866	HORVU5Hr1G011270	851:866	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	7	63	theme	candidate	792:800	arg1	HORVU5Hr1G011230					833:848	HORVU5Hr1G011230	833:848	HORVU5Hr1G011230	833:848	Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly.					
33430526	10	64	theme	genetic	1392:1398	arg1	improvement					1400:1410	genetic improvement	1392:1410	genetic improvement of grains starch in barley	1392:1437	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33430526	10	65	theme	grains	1415:1420	arg1	improvement					1400:1410	genetic improvement	1392:1410	genetic improvement of grains starch in barley	1392:1437	In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.					
33320638	2	0	theme	human	512:516	arg1	protein					535:541	human VE-cad-Fc fusion protein	512:541	human VE-cad-Fc fusion protein	512:541	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	5	1	theme	inflammatory	1090:1101	arg1	cytokines					1103:1111	inflammatory cytokines	1090:1111	inflammatory cytokines	1090:1111	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	2	2	theme	functionalized	628:641	arg1	aggregates					647:656	functionalized MSC aggregates	628:656	functionalized MSC aggregates (FMAs)	628:663	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	2	2	theme	functionalized	628:641	arg1	FMAs					659:662	FMAs	659:662	FMAs	659:662	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	6	3	from	revascularization	1372:1388	arg1	myocardium					1405:1414	the infarct myocardium	1393:1414	the infarct myocardium	1393:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	1	4	theme	injection	175:183	arg1	strategies					151:160	Conventional strategies	138:160	Conventional strategies of stem cell injection in treating myocardial infarction (MI)	138:222	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	1	5	theme	retention	258:266	arg1	rate					268:271	low retention rate	254:271	low retention rate	254:271	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	0	6	theme	Cell	86:89	arg1	Aggregates					91:100	Functionalized Human Mesenchymal Stem Cell Aggregates	48:100	Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium	48:135	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	5	7	theme	cytokines	1103:1111	arg1	expressions					1075:1085	decreased expressions	1065:1085	decreased expressions of inflammatory cytokines	1065:1111	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	6	8	theme	histological	1271:1282	arg1	analyses					1284:1291	histological analyses	1271:1291	histological analyses	1271:1291	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	6	9	theme	echocardiography	1250:1265	arg1	results					1234:1240	The results	1230:1240	The results of both echocardiography and histological analyses	1230:1291	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	4	10	theme	oxidized	872:879	arg1	OHA					885:887	OHA	885:887	OHA	885:887	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	4	10	theme	oxidized	872:879	arg1	HA					881:882	oxidized HA	872:882	oxidized HA (OHA)	872:888	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	1	11	theme	infarcted	377:385	arg1	myocardium					387:396	the infarcted myocardium	373:396	the infarcted myocardium	373:396	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	4	12	theme	hyaluronic	785:794	arg1	HA					802:803	HA	802:803	HA	802:803	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	4	12	theme	hyaluronic	785:794	arg1	acid					796:799	injectable hyaluronic acid	774:799	an injectable hyaluronic acid (HA)-based hydrogel	771:819	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	7	13	theme	other	1583:1587	arg1	repair					1596:1601	other tissue repair	1583:1601	other tissue repair	1583:1601	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	6	14	theme	revascularization	1372:1388	arg1	reconstruction					1319:1332	the efficient reconstruction	1305:1332	the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium	1305:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	5	15	theme	hydrogel	927:934	arg1	FMA					944:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	4	16	theme	injectable	774:783	arg1	HA					802:803	HA	802:803	HA	802:803	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	4	16	theme	injectable	774:783	arg1	acid					796:799	injectable hyaluronic acid	774:799	an injectable hyaluronic acid (HA)-based hydrogel	771:819	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	1	17	theme	insufficient	277:288	arg1	secretion					290:298	insufficient secretion	277:298	insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium	277:396	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	1	18	from	microenvironment	353:368	arg1	myocardium					387:396	the infarcted myocardium	373:396	the infarcted myocardium	373:396	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	6	19	from	structure	1358:1366	arg1	myocardium					1405:1414	the infarct myocardium	1393:1414	the infarct myocardium	1393:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	4	20	theme	Schiff	843:848	arg1	reaction					855:862	Schiff base reaction	843:862	Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA)	843:912	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	5	21	theme	loading	936:942	arg1	FMA					944:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	6	22	theme	analyses	1284:1291	arg1	results					1234:1240	The results	1230:1240	The results of both echocardiography and histological analyses	1230:1291	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	6	23	theme	infarct	1397:1403	arg1	myocardium					1405:1414	the infarct myocardium	1393:1414	the infarct myocardium	1393:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	0	24	theme	Infarcted	116:124	arg1	Myocardium					126:135	Infarcted Myocardium	116:135	Infarcted Myocardium	116:135	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	3	25	theme	MSCs	736:739	arg1	activity					724:731	the paracrine activity	710:731	the paracrine activity of MSCs	710:739	This fusion protein can effectively promote the paracrine activity of MSCs.					
33320638	5	26	theme	plain	1177:1181	arg1	hydrogel					1183:1190	the plain hydrogel	1173:1190	the plain hydrogel only	1173:1195	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	6	27	from	function	1345:1352	arg1	myocardium					1405:1414	the infarct myocardium	1393:1414	the infarct myocardium	1393:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	4	28	theme	acid	796:799	arg1	hydrogel					812:819	an injectable hyaluronic acid (HA)-based hydrogel	771:819	an injectable hyaluronic acid (HA)-based hydrogel	771:819	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	1	29	theme	myocardial	197:206	arg1	infarction					208:217	myocardial infarction	197:217	myocardial infarction (MI)	197:222	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	1	29	theme	myocardial	197:206	arg1	MI					220:221	MI	220:221	MI	220:221	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	0	30	theme	Hyaluronic	11:20	arg1	Hydrogel					27:34	Injectable Hyaluronic Acid Hydrogel	0:34	Injectable Hyaluronic Acid Hydrogel	0:34	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	5	31	theme	expressions	1075:1085	arg1	terms					1056:1060	terms	1056:1060	terms of decreased expressions of inflammatory cytokines	1056:1111	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	2	32	theme	fusion	528:533	arg1	protein					535:541	human VE-cad-Fc fusion protein	512:541	human VE-cad-Fc fusion protein	512:541	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	7	33	theme	cell	1453:1456	arg1	aggregates					1458:1467	functionalized stem cell aggregates	1433:1467	functionalized stem cell aggregates with an injectable hydrogel	1433:1495	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	3	34	theme	paracrine	714:722	arg1	activity					724:731	the paracrine activity	710:731	the paracrine activity of MSCs	710:739	This fusion protein can effectively promote the paracrine activity of MSCs.					
33320638	0	35	theme	Injectable	0:9	arg1	Hydrogel					27:34	Injectable Hyaluronic Acid Hydrogel	0:34	Injectable Hyaluronic Acid Hydrogel	0:34	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	5	36	theme	factors	1153:1159	arg1	secretion					1129:1137	secretion	1129:1137	secretion of angiogenic factors	1129:1159	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	2	37	theme	MSC	643:645	arg1	aggregates					647:656	functionalized MSC aggregates	628:656	functionalized MSC aggregates (FMAs)	628:663	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	2	37	theme	MSC	643:645	arg1	FMAs					659:662	FMAs	659:662	FMAs	659:662	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	7	38	theme	functionalized	1433:1446	arg1	aggregates					1458:1467	functionalized stem cell aggregates	1433:1467	functionalized stem cell aggregates with an injectable hydrogel	1433:1495	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	6	39	theme	function	1345:1352	arg1	reconstruction					1319:1332	the efficient reconstruction	1305:1332	the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium	1305:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	3	40	theme	fusion	671:676	arg1	protein					678:684	This fusion protein	666:684	This fusion protein	666:684	This fusion protein can effectively promote the paracrine activity of MSCs.					
33320638	7	41	theme	aggregates	1458:1467	arg1	delivery					1421:1428	The delivery	1417:1428	The delivery of functionalized stem cell aggregates with an injectable hydrogel	1417:1495	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	2	42	theme	mesenchymal	584:594	arg1	cells					601:605	human mesenchymal stem cells	578:605	human mesenchymal stem cells (hMSCs)	578:613	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	2	42	theme	mesenchymal	584:594	arg1	hMSCs					608:612	hMSCs	608:612	hMSCs	608:612	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	6	43	theme	structure	1358:1366	arg1	reconstruction					1319:1332	the efficient reconstruction	1305:1332	the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium	1305:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	5	44	theme	OHA	919:921	arg1	FMA					944:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	0	45	theme	Acid	22:25	arg1	Hydrogel					27:34	Injectable Hyaluronic Acid Hydrogel	0:34	Injectable Hyaluronic Acid Hydrogel	0:34	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	1	46	theme	exogenous	303:311	arg1	cytokines					313:321	exogenous cytokines	303:321	exogenous cytokines	303:321	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	2	47	theme	human	578:582	arg1	cells					601:605	human mesenchymal stem cells	578:605	human mesenchymal stem cells (hMSCs)	578:613	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	2	47	theme	human	578:582	arg1	hMSCs					608:612	hMSCs	608:612	hMSCs	608:612	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	5	48	theme	@	922:922	arg1	FMA					944:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	2	49	theme	poly	446:449	arg1	microparticles					483:496	poly(lactic-co-glycolic acid) (PLGA) microparticles	446:496	poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein	446:541	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	1	50	theme	cytokines	313:321	arg1	rate					268:271	low retention rate	254:271	low retention rate	254:271	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	1	50	theme	cytokines	313:321	arg1	secretion					290:298	insufficient secretion	277:298	insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium	277:396	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	6	51	theme	cardiac	1337:1343	arg1	function					1345:1352	cardiac function	1337:1352	cardiac function	1337:1352	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	1	52	theme	therapeutic	418:428	arg1	effect					430:435	the therapeutic effect	414:435	the therapeutic effect	414:435	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	5	53	theme	infarcted	969:977	arg1	myocardium					979:988	the infarcted myocardium	965:988	the infarcted myocardium of rats	965:996	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	7	54	theme	injectable	1477:1486	arg1	hydrogel					1488:1495	an injectable hydrogel	1474:1495	an injectable hydrogel	1474:1495	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	5	55	theme	angiogenic	1142:1151	arg1	factors					1153:1159	angiogenic factors	1142:1159	angiogenic factors	1142:1159	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	0	56	theme	Human	63:67	arg1	Aggregates					91:100	Functionalized Human Mesenchymal Stem Cell Aggregates	48:100	Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium	48:135	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	2	57	theme	VE-cad-Fc	518:526	arg1	protein					535:541	human VE-cad-Fc fusion protein	512:541	human VE-cad-Fc fusion protein	512:541	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	4	58	theme	base	850:853	arg1	reaction					855:862	Schiff base reaction	843:862	Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA)	843:912	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	0	59	theme	Functionalized	48:61	arg1	Aggregates					91:100	Functionalized Human Mesenchymal Stem Cell Aggregates	48:100	Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium	48:135	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	6	60	from	reconstruction	1319:1332	arg1	myocardium					1405:1414	the infarct myocardium	1393:1414	the infarct myocardium	1393:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	6	61	theme	efficient	1309:1317	arg1	reconstruction					1319:1332	the efficient reconstruction	1305:1332	the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium	1305:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	5	62	theme	rats	993:996	arg1	myocardium					979:988	the infarcted myocardium	965:988	the infarcted myocardium of rats	965:996	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	1	63	theme	Conventional	138:149	arg1	strategies					151:160	Conventional strategies	138:160	Conventional strategies of stem cell injection in treating myocardial infarction (MI)	138:222	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	4	64	theme	-based	805:810	arg1	hydrogel					812:819	an injectable hyaluronic acid (HA)-based hydrogel	771:819	an injectable hyaluronic acid (HA)-based hydrogel	771:819	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	4	65	theme	hydrazided	894:903	arg1	HHA					909:911	HHA	909:911	HHA	909:911	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	4	65	theme	hydrazided	894:903	arg1	HA					905:906	hydrazided HA	894:906	hydrazided HA (HHA)	894:912	The FMA is encapsulated with an injectable hyaluronic acid (HA)-based hydrogel, which is prepared by Schiff base reaction between oxidized HA (OHA) and hydrazided HA (HHA).					
33320638	7	66	theme	stem	1448:1451	arg1	aggregates					1458:1467	functionalized stem cell aggregates	1433:1467	functionalized stem cell aggregates with an injectable hydrogel	1433:1495	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	7	67	theme	promising	1506:1514	arg1	strategy					1516:1523	a promising strategy	1504:1523	a promising strategy for treating myocardial infarction	1504:1558	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	6	68	from	myocardium	1405:1414	arg1	reconstruction					1319:1332	the efficient reconstruction	1305:1332	the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium	1305:1414	The results of both echocardiography and histological analyses demonstrate the efficient reconstruction of cardiac function and structure and revascularization in the infarct myocardium.					
33320638	2	69	theme	stem	596:599	arg1	cells					601:605	human mesenchymal stem cells	578:605	human mesenchymal stem cells (hMSCs)	578:613	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	2	69	theme	stem	596:599	arg1	hMSCs					608:612	hMSCs	608:612	hMSCs	608:612	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	5	70	theme	HHA	923:925	arg1	FMA					944:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA	915:946	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	5	71	theme	MI	1033:1034	arg1	microenvironment					1036:1051	the MI microenvironment	1029:1051	the MI microenvironment	1029:1051	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	0	72	theme	Stem	81:84	arg1	Aggregates					91:100	Functionalized Human Mesenchymal Stem Cell Aggregates	48:100	Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium	48:135	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	7	73	theme	myocardial	1538:1547	arg1	infarction					1549:1558	myocardial infarction	1538:1558	myocardial infarction	1538:1558	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	7	74	theme	tissue	1589:1594	arg1	repair					1596:1601	other tissue repair	1583:1601	other tissue repair	1583:1601	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	1	75	theme	stem	165:168	arg1	injection					175:183	stem cell injection	165:183	stem cell injection in treating myocardial infarction (MI)	165:222	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	7	76	with	aggregates	1458:1467	arg1	hydrogel					1488:1495	an injectable hydrogel	1474:1495	an injectable hydrogel	1474:1495	The delivery of functionalized stem cell aggregates with an injectable hydrogel offers a promising strategy for treating myocardial infarction and may be expanded to other tissue repair and reconstruction.					
33320638	0	77	theme	Mesenchymal	69:79	arg1	Aggregates					91:100	Functionalized Human Mesenchymal Stem Cell Aggregates	48:100	Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium	48:135	Injectable Hyaluronic Acid Hydrogel Loaded with Functionalized Human Mesenchymal Stem Cell Aggregates for Repairing Infarcted Myocardium.					
33320638	2	78	theme	lactic-co-glycolic	451:468	arg1	poly					446:449	poly	446:449	poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein	446:541	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	2	78	theme	lactic-co-glycolic	451:468	arg1	acid					470:473	lactic-co-glycolic acid	451:473	lactic-co-glycolic acid	451:473	Herein, poly(lactic-co-glycolic acid) (PLGA) microparticles modified with human VE-cad-Fc fusion protein are fabricated and integrated with human mesenchymal stem cells (hMSCs) to construct functionalized MSC aggregates (FMAs).					
33320638	5	79	theme	decreased	1065:1073	arg1	expressions					1075:1085	decreased expressions	1065:1085	decreased expressions of inflammatory cytokines	1065:1111	The OHA@HHA hydrogel loading FMA is injected into the infarcted myocardium of rats, thereby efficiently improving the MI microenvironment in terms of decreased expressions of inflammatory cytokines and upregulated secretion of angiogenic factors compared to the plain hydrogel only and hydrogel encapsulating MSCs.					
33320638	1	80	theme	cell	170:173	arg1	injection					175:183	stem cell injection	165:183	stem cell injection in treating myocardial infarction (MI)	165:222	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
33320638	1	81	theme	low	254:256	arg1	rate					268:271	low retention rate	254:271	low retention rate	254:271	Conventional strategies of stem cell injection in treating myocardial infarction (MI) remain a challenge because of low retention rate and insufficient secretion of exogenous cytokines for efficiently improving the microenvironment in the infarcted myocardium, thus hampering the therapeutic effect.					
34003690	1	0	with	patients	192:199	arg1	fraction					245:252	reduced ejection fraction	228:252	reduced ejection fraction	228:252	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	4	1	theme	randomized	848:857	arg1	multicenter					835:845	a multicenter	833:845	a multicenter	833:845	HEART-FID is a multicenter, randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers.					
34003690	4	1	theme	randomized	848:857	arg1	trial					893:897	randomized, double-blind, placebo-controlled trial	848:897	randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers	848:952	HEART-FID is a multicenter, randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers.					
34003690	1	2	contain	has	154:156	arg1	ID					150:151	ID	150:151	ID	150:151	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	1	2	contain	has	154:156	arg1	deficiency					138:147	BACKGROUND Iron deficiency	122:147	BACKGROUND Iron deficiency (ID)	122:152	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	1	2	contain	has	154:156	arg2	prevalence					160:169	a prevalence	158:169	a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction	158:252	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	5	3	from	years	986:990	arg1	HF					1010:1011	stable chronic HF	995:1011	stable chronic HF	995:1011	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	4	theme	transferrin	1162:1172	arg1	saturation					1174:1183	a transferrin saturation	1160:1183	a transferrin saturation <20%	1160:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	5	dep	METHODS	561:567	arg1	is					668:669	is	668:669	is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID	668:817	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	3	6	theme	carboxymaltose	711:724	arg1	efficacy					681:688	efficacy	681:688	efficacy	681:688	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	3	6	theme	carboxymaltose	711:724	arg1	safety					694:699	safety	694:699	safety	694:699	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	5	7	with	ng/mL	1123:1127	arg1	saturation					1174:1183	a transferrin saturation	1160:1183	a transferrin saturation <20%	1160:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	8	8	theme	HEART-FID	1680:1688	arg1	trial					1690:1694	The HEART-FID trial	1676:1694	The HEART-FID trial	1676:1694	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	1	9	theme	≈40	174:176	arg1	%					177:177	%	177:177	%	177:177	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	8	10	theme	symptomatic	1861:1871	arg1	HF					1873:1874	symptomatic HF	1861:1874	symptomatic HF	1861:1874	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	5	11	theme	functional	1045:1054	arg1	class					1056:1060	New York Heart Association functional class II to IV	1018:1069	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	12	with	Carboxymaltose	614:627	arg1	Deficiency					656:665	Iron Deficiency	651:665	Iron Deficiency	651:665	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	7	13	from	baseline	1607:1614	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	13	from	baseline	1607:1614	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	13	from	baseline	1607:1614	arg1	change					1595:1600	change	1595:1600	change from baseline to 6 months in the 6-minute walk test distance	1595:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	13	from	baseline	1607:1614	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	13	from	baseline	1607:1614	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	5	14	dep	aged	977:980	arg1	years					986:990	≥18 years	982:990	≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization	982:1224	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	14	dep	aged	977:980	arg1	patients					964:971	Eligible patients	955:971	Eligible patients	955:971	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	6	15	theme	Consented	1280:1288	arg1	patients					1290:1297	Consented patients	1280:1297	Consented patients	1280:1297	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	8	16	theme	clinical	1708:1715	arg1	practice					1717:1724	clinical practice	1708:1724	clinical practice	1708:1724	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	1	17	theme	%	184:184	arg1	prevalence					160:169	a prevalence	158:169	a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction	158:252	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	0	18	from	Failure	70:76	arg1	Trial					30:34	Randomized Placebo-Controlled Trial	0:34	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.	0:120	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	8	19	theme	ejection	1889:1896	arg1	fraction					1898:1905	reduced ejection fraction	1881:1905	reduced ejection fraction with ID	1881:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	5	20	theme	New	1018:1020	arg1	class					1056:1060	New York Heart Association functional class II to IV	1018:1069	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	21	with	fraction	802:809	arg1	ID					816:817	ID	816:817	ID	816:817	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	1	22	theme	BACKGROUND	122:131	arg1	ID					150:151	ID	150:151	ID	150:151	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	1	22	theme	BACKGROUND	122:131	arg1	deficiency					138:147	BACKGROUND Iron deficiency	122:147	BACKGROUND Iron deficiency (ID)	122:152	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	7	23	theme	hospitalization	1562:1576	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	23	theme	hospitalization	1562:1576	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	23	theme	hospitalization	1562:1576	arg1	change					1595:1600	change	1595:1600	change from baseline to 6 months in the 6-minute walk test distance	1595:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	23	theme	hospitalization	1562:1576	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	23	theme	hospitalization	1562:1576	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	8	24	with	fraction	1898:1905	arg1	ID					1912:1913	ID	1912:1913	ID	1912:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	1	25	from	patients	192:199	arg1	HF					219:220	HF	219:220	HF	219:220	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	1	25	from	patients	192:199	arg1	failure					210:216	heart failure	204:216	heart failure (HF)	204:221	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	5	26	theme	Heart	1027:1031	arg1	class					1056:1060	New York Heart Association functional class II to IV	1018:1069	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	27	theme	trial	600:604	arg1	objective					573:581	The objective	569:581	The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency)	569:666	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	8	28	with	patients	1847:1854	arg1	fraction					1898:1905	reduced ejection fraction	1881:1905	reduced ejection fraction with ID	1881:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	8	28	with	patients	1847:1854	arg1	HF					1873:1874	symptomatic HF	1861:1874	symptomatic HF	1861:1874	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	2	29	theme	reduced	480:486	arg1	fraction					497:504	reduced ejection fraction	480:504	reduced ejection fraction with ID	480:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	5	30	theme	N-terminal	1238:1247	arg1	peptide					1271:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	1	31	theme	worse	277:281	arg1	prognosis					283:291	worse prognosis	277:291	worse prognosis	277:291	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	2	32	with	patients	458:465	arg1	fraction					497:504	reduced ejection fraction	480:504	reduced ejection fraction with ID	480:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	2	32	with	patients	458:465	arg1	HF					472:473	HF	472:473	HF	472:473	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	0	33	from	Trial	30:34	arg1	Failure					70:76	Heart Failure	64:76	Heart Failure	64:76	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	0	34	theme	Iron	83:86	arg1	Deficiency					88:97	Iron Deficiency	83:97	Iron Deficiency	83:97	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	5	35	theme	%	1102:1102	arg1	ID					1105:1106	ID	1105:1106	ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%)	1105:1189	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	35	theme	%	1102:1102	arg1	symptoms					1071:1078	New York Heart Association functional class II to IV symptoms	1018:1078	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	35	theme	%	1102:1102	arg1	fraction					1090:1097	ejection fraction	1081:1097	ejection fraction ≤40%	1081:1102	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	8	36	theme	treatment	1762:1770	arg1	role					1744:1747	the role	1740:1747	the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID	1740:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	7	37	theme	test	1649:1652	arg1	distance					1654:1661	the 6-minute walk test distance	1631:1661	the 6-minute walk test distance	1631:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	38	theme	hierarchical	1523:1534	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	38	theme	hierarchical	1523:1534	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	38	theme	hierarchical	1523:1534	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	38	theme	hierarchical	1523:1534	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	9	39	theme	Unique	1967:1972	arg1	identifier					1974:1983	Unique identifier	1967:1983	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.	1916:1997	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.					
34003690	5	40	theme	HF	1207:1208	arg1	symptoms					1071:1078	New York Heart Association functional class II to IV symptoms	1018:1078	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	40	theme	HF	1207:1208	arg1	hospitalization					1210:1224	documented HF hospitalization	1196:1224	documented HF hospitalization	1196:1224	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	41	theme	reduced	785:791	arg1	fraction					802:809	reduced ejection fraction	785:809	reduced ejection fraction with ID	785:817	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	8	42	theme	intravenous	1777:1787	arg1	carboxymaltose					1796:1809	intravenous ferric carboxymaltose	1777:1809	intravenous ferric carboxymaltose	1777:1809	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	5	43	theme	Eligible	955:962	arg1	years					986:990	≥18 years	982:990	≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization	982:1224	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	43	theme	Eligible	955:962	arg1	patients					964:971	Eligible patients	955:971	Eligible patients	955:971	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	7	44	theme	6-minute	1635:1642	arg1	distance					1654:1661	the 6-minute walk test distance	1631:1661	the 6-minute walk test distance	1631:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	0	45	theme	Carboxymaltose	46:59	arg1	Trial					30:34	Randomized Placebo-Controlled Trial	0:34	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.	0:120	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	2	46	with	fraction	497:504	arg1	ID					511:512	ID	511:512	ID	511:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	0	47	theme	Randomized	0:9	arg1	Trial					30:34	Randomized Placebo-Controlled Trial	0:34	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.	0:120	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	6	48	dep	baseline	1351:1358	arg1	months					1402:1407	every 6 months	1394:1407	every 6 months	1394:1407	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	2	49	theme	patient-reported	405:420	arg1	outcomes					422:429	patient-reported outcomes	405:429	patient-reported outcomes	405:429	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	5	50	theme	natriuretic	1259:1269	arg1	peptide					1271:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	51	theme	ferritin	1132:1139	arg1	ng/mL					1149:1153	ferritin 100-300 ng/mL	1132:1153	ferritin 100-300 ng/mL with a transferrin saturation <20%	1132:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	7	52	theme	primary	1500:1506	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	52	theme	primary	1500:1506	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	52	theme	primary	1500:1506	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	52	theme	primary	1500:1506	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	0	53	theme	Ferric	39:44	arg1	Carboxymaltose					46:59	Ferric Carboxymaltose	39:59	Ferric Carboxymaltose in Heart Failure	39:76	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	5	54	theme	chronic	1002:1008	arg1	HF					1010:1011	stable chronic HF	995:1011	stable chronic HF	995:1011	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	55	theme	Ferric	607:612	arg1	Carboxymaltose					614:627	Ferric Carboxymaltose	607:627	Ferric Carboxymaltose in Heart Failure With Iron Deficiency	607:665	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	0	56	with	Trial	30:34	arg1	Deficiency					88:97	Iron Deficiency	83:97	Iron Deficiency	83:97	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	2	57	theme	Several	294:300	arg1	trials					302:307	Several trials	294:307	Several trials	294:307	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	5	58	theme	ferritin	1109:1116	arg1	ng/mL					1123:1127	ferritin <100 ng/mL	1109:1127	ferritin <100 ng/mL	1109:1127	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	59	theme	Iron	651:654	arg1	Deficiency					656:665	Iron Deficiency	651:665	Iron Deficiency	651:665	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	6	60	theme	iron	1459:1462	arg1	indices					1464:1470	hemoglobin and iron indices	1444:1470	hemoglobin and iron indices for the trial duration	1444:1493	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	0	61	dep	Trial	30:34	arg1	Rationale					100:108	Rationale	100:108	Rationale	100:108	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	0	61	dep	Trial	30:34	arg1	Design					114:119	Design	114:119	Design	114:119	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	5	62	with	years	986:990	arg1	fraction					1090:1097	ejection fraction	1081:1097	ejection fraction ≤40%	1081:1102	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	62	with	years	986:990	arg1	symptoms					1071:1078	New York Heart Association functional class II to IV symptoms	1018:1078	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	62	with	years	986:990	arg1	hospitalization					1210:1224	documented HF hospitalization	1196:1224	documented HF hospitalization	1196:1224	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	1	63	dep	%	177:177	arg1	to					179:180	to	179:180	to	179:180	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	2	64	theme	intravenous	327:337	arg1	carboxymaltose					346:359	intravenous ferric carboxymaltose	327:359	intravenous ferric carboxymaltose	327:359	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	6	65	theme	hemoglobin	1444:1453	arg1	indices					1464:1470	hemoglobin and iron indices	1444:1470	hemoglobin and iron indices for the trial duration	1444:1493	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	5	66	theme	%	1188:1188	arg1	saturation					1174:1183	a transferrin saturation	1160:1183	a transferrin saturation <20%	1160:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	6	67	theme	repeated	1366:1373	arg1	visits/assessments					1375:1392	repeated visits/assessments	1366:1392	repeated visits/assessments	1366:1392	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	2	68	from	improvement	390:400	arg1	outcomes					422:429	patient-reported outcomes	405:429	patient-reported outcomes	405:429	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	2	68	from	improvement	390:400	arg1	patients					458:465	patients	458:465	patients with HF with reduced ejection fraction with ID	458:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	5	69	dep	class	1056:1060	arg1	to					1065:1066	to	1065:1066	to	1065:1066	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	8	70	with	treatment	1762:1770	arg1	carboxymaltose					1796:1809	intravenous ferric carboxymaltose	1777:1809	intravenous ferric carboxymaltose	1777:1809	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	8	71	theme	usual	1821:1825	arg1	care					1827:1830	usual care	1821:1830	usual care	1821:1830	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	6	72	theme	study	1424:1428	arg1	drug					1430:1433	additional study drug	1413:1433	additional study drug based on hemoglobin and iron indices for the trial duration	1413:1493	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	3	73	from	Carboxymaltose	614:627	arg1	Failure					638:644	Heart Failure	632:644	Heart Failure	632:644	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	8	74	theme	ambulatory	1836:1845	arg1	patients					1847:1854	ambulatory patients	1836:1854	ambulatory patients with symptomatic HF with reduced ejection fraction with ID	1836:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	9	75	dep	URL	1930:1932	arg1	identifier					1974:1983	Unique identifier	1967:1983	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.	1916:1997	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.					
34003690	9	75	dep	URL	1930:1932	arg1	https					1935:1939	https	1935:1939	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.	1916:1997	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.					
34003690	9	75	dep	URL	1930:1932	arg1	NCT03037931					1986:1996	NCT03037931	1986:1996	NCT03037931	1986:1996	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.					
34003690	9	75	dep	URL	1930:1932	arg1	//www.clinicaltrials.gov					1941:1964	//www.clinicaltrials.gov	1941:1964	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.	1916:1997	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.					
34003690	9	76	dep	Registration	1916:1927	arg1	URL					1930:1932	URL	1930:1932	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.	1916:1997	Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03037931.					
34003690	6	77	theme	trial	1480:1484	arg1	duration					1486:1493	the trial duration	1476:1493	the trial duration	1476:1493	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	2	78	theme	morbidity	519:527	arg1	data					543:546	morbidity and mortality data	519:546	morbidity and mortality data	519:546	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	8	79	from	role	1744:1747	arg1	patients					1847:1854	ambulatory patients	1836:1854	ambulatory patients with symptomatic HF with reduced ejection fraction with ID	1836:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	3	80	theme	ferric	704:709	arg1	carboxymaltose					711:724	ferric carboxymaltose	704:724	ferric carboxymaltose	704:724	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	8	81	theme	reduced	1881:1887	arg1	fraction					1898:1905	reduced ejection fraction	1881:1905	reduced ejection fraction with ID	1881:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	5	82	theme	class	1056:1060	arg1	fraction					1090:1097	ejection fraction	1081:1097	ejection fraction ≤40%	1081:1102	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	82	theme	class	1056:1060	arg1	symptoms					1071:1078	New York Heart Association functional class II to IV symptoms	1018:1078	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	82	theme	class	1056:1060	arg1	hospitalization					1210:1224	documented HF hospitalization	1196:1224	documented HF hospitalization	1196:1224	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	1	83	theme	50	182:183	arg1	%					177:177	%	177:177	%	177:177	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	4	84	theme	≈3014	909:913	arg1	patients					915:922	≈3014 patients	909:922	≈3014 patients	909:922	HEART-FID is a multicenter, randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers.					
34003690	7	85	theme	HF	1559:1560	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	86	from	months	1621:1626	arg1	distance					1654:1661	the 6-minute walk test distance	1631:1661	the 6-minute walk test distance	1631:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	5	87	theme	York	1022:1025	arg1	class					1056:1060	New York Heart Association functional class II to IV	1018:1069	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	88	theme	HEART-FID	590:598	arg1	trial					600:604	the HEART-FID trial	586:604	the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency)	586:666	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	6	89	with	baseline	1351:1358	arg1	visits/assessments					1375:1392	repeated visits/assessments	1366:1392	repeated visits/assessments	1366:1392	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	1	90	theme	Iron	133:136	arg1	ID					150:151	ID	150:151	ID	150:151	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	1	90	theme	Iron	133:136	arg1	deficiency					138:147	BACKGROUND Iron deficiency	122:147	BACKGROUND Iron deficiency (ID)	122:152	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	7	91	theme	death	1549:1553	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	91	theme	death	1549:1553	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	91	theme	death	1549:1553	arg1	change					1595:1600	change	1595:1600	change from baseline to 6 months in the 6-minute walk test distance	1595:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	91	theme	death	1549:1553	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	91	theme	death	1549:1553	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	2	92	theme	mortality	533:541	arg1	data					543:546	morbidity and mortality data	519:546	morbidity and mortality data	519:546	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	5	93	theme	elevated	1229:1236	arg1	peptide					1271:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	94	theme	Association	1033:1043	arg1	class					1056:1060	New York Heart Association functional class II to IV	1018:1069	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	4	95	theme	international	932:944	arg1	centers					946:952	≈300 international centers	927:952	≈300 international centers	927:952	HEART-FID is a multicenter, randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers.					
34003690	3	96	theme	symptomatic	765:775	arg1	HF					777:778	symptomatic HF	765:778	symptomatic HF	765:778	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	5	97	theme	≤40	1099:1101	arg1	%					1102:1102	%	1102:1102	%	1102:1102	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	98	theme	documented	1196:1205	arg1	symptoms					1071:1078	New York Heart Association functional class II to IV symptoms	1018:1078	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	98	theme	documented	1196:1205	arg1	hospitalization					1210:1224	documented HF hospitalization	1196:1224	documented HF hospitalization	1196:1224	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	6	99	from	baseline	1351:1358	arg1	carboxymaltose					1322:1335	ferric carboxymaltose	1315:1335	ferric carboxymaltose	1315:1335	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	6	99	from	baseline	1351:1358	arg1	placebo					1340:1346	placebo	1340:1346	placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration	1340:1493	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	8	100	theme	long-term	1752:1760	arg1	treatment					1762:1770	long-term treatment	1752:1770	long-term treatment with intravenous ferric carboxymaltose, added to usual care,	1752:1831	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	2	101	from	capacity	446:453	arg1	outcomes					422:429	patient-reported outcomes	405:429	patient-reported outcomes	405:429	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	2	101	from	capacity	446:453	arg1	patients					458:465	patients	458:465	patients with HF with reduced ejection fraction with ID	458:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	3	102	theme	ejection	793:800	arg1	fraction					802:809	reduced ejection fraction	785:809	reduced ejection fraction with ID	785:817	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	1	103	theme	ejection	236:243	arg1	fraction					245:252	reduced ejection fraction	228:252	reduced ejection fraction	228:252	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	7	104	from	months	1584:1589	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	104	from	months	1584:1589	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	104	from	months	1584:1589	arg1	change					1595:1600	change	1595:1600	change from baseline to 6 months in the 6-minute walk test distance	1595:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	104	from	months	1584:1589	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	104	from	months	1584:1589	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	3	105	with	treatment	751:759	arg1	fraction					802:809	reduced ejection fraction	785:809	reduced ejection fraction with ID	785:817	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	7	106	theme	walk	1644:1647	arg1	distance					1654:1661	the 6-minute walk test distance	1631:1661	the 6-minute walk test distance	1631:1661	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	2	107	theme	ejection	488:495	arg1	fraction					497:504	reduced ejection fraction	480:504	reduced ejection fraction with ID	480:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	0	108	from	Carboxymaltose	46:59	arg1	Failure					70:76	Heart Failure	64:76	Heart Failure	64:76	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	8	109	dep	CONCLUSIONS	1664:1674	arg1	inform					1701:1706	inform	1701:1706	will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID	1696:1913	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	6	110	theme	ferric	1315:1320	arg1	carboxymaltose					1322:1335	ferric carboxymaltose	1315:1335	ferric carboxymaltose	1315:1335	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	0	111	theme	Placebo-Controlled	11:28	arg1	Trial					30:34	Randomized Placebo-Controlled Trial	0:34	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.	0:120	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	5	112	theme	ejection	1081:1088	arg1	ID					1105:1106	ID	1105:1106	ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%)	1105:1189	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	112	theme	ejection	1081:1088	arg1	symptoms					1071:1078	New York Heart Association functional class II to IV symptoms	1018:1078	New York Heart Association functional class II to IV symptoms	1018:1078	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	112	theme	ejection	1081:1088	arg1	fraction					1090:1097	ejection fraction	1081:1097	ejection fraction ≤40%	1081:1102	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	7	113	from	change	1595:1600	arg1	months					1584:1589	12 months	1581:1589	12 months	1581:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	8	114	theme	ferric	1789:1794	arg1	carboxymaltose					1796:1809	intravenous ferric carboxymaltose	1777:1809	intravenous ferric carboxymaltose	1777:1809	CONCLUSIONS The HEART-FID trial will inform clinical practice by clarifying the role of long-term treatment with intravenous ferric carboxymaltose, added to usual care, in ambulatory patients with symptomatic HF with reduced ejection fraction with ID.					
34003690	5	115	theme	pro-brain	1249:1257	arg1	peptide					1271:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	elevated N-terminal pro-brain natriuretic peptide	1229:1277	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	2	116	theme	ferric	339:344	arg1	carboxymaltose					346:359	intravenous ferric carboxymaltose	327:359	intravenous ferric carboxymaltose	327:359	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	1	117	theme	reduced	228:234	arg1	fraction					245:252	reduced ejection fraction	228:252	reduced ejection fraction	228:252	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	2	118	theme	sustained	380:388	arg1	improvement					390:400	early and sustained improvement	370:400	early and sustained improvement in patient-reported outcomes	370:429	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	4	119	dep	randomized	848:857	arg1	placebo-controlled					874:891	placebo-controlled	874:891	placebo-controlled	874:891	HEART-FID is a multicenter, randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers.					
34003690	4	119	dep	randomized	848:857	arg1	double-blind					860:871	double-blind	860:871	double-blind	860:871	HEART-FID is a multicenter, randomized, double-blind, placebo-controlled trial enrolling ≈3014 patients at ≈300 international centers.					
34003690	7	120	theme	end	1508:1510	arg1	point					1512:1516	The primary end point	1496:1516	The primary end point	1496:1516	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	120	theme	end	1508:1510	arg1	death					1549:1553	death	1549:1553	death	1549:1553	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	120	theme	end	1508:1510	arg1	hospitalization					1562:1576	HF hospitalization	1559:1576	HF hospitalization	1559:1576	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	7	120	theme	end	1508:1510	arg1	composite					1536:1544	a hierarchical composite	1521:1544	a hierarchical composite of death and HF hospitalization at 12 months	1521:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	5	121	theme	stable	995:1000	arg1	HF					1010:1011	stable chronic HF	995:1011	stable chronic HF	995:1011	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	2	122	theme	early	370:374	arg1	improvement					390:400	early and sustained improvement	370:400	early and sustained improvement in patient-reported outcomes	370:429	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	0	123	theme	Heart	64:68	arg1	Failure					70:76	Heart Failure	64:76	Heart Failure	64:76	Randomized Placebo-Controlled Trial of Ferric Carboxymaltose in Heart Failure With Iron Deficiency: Rationale and Design.					
34003690	7	124	from	composite	1536:1544	arg1	months					1584:1589	12 months	1581:1589	12 months	1581:1589	The primary end point is a hierarchical composite of death and HF hospitalization at 12 months and change from baseline to 6 months in the 6-minute walk test distance.					
34003690	5	125	dep	ID	1105:1106	arg1	ng/mL					1149:1153	ferritin 100-300 ng/mL	1132:1153	ferritin 100-300 ng/mL with a transferrin saturation <20%	1132:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	5	125	dep	ID	1105:1106	arg1	ng/mL					1123:1127	ferritin <100 ng/mL	1109:1127	ferritin <100 ng/mL	1109:1127	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	2	126	theme	functional	435:444	arg1	capacity					446:453	functional capacity	435:453	functional capacity in patients with HF with reduced ejection fraction with ID	435:512	Several trials demonstrated that intravenous ferric carboxymaltose leads to early and sustained improvement in patient-reported outcomes and functional capacity in patients with HF with reduced ejection fraction with ID, yet morbidity and mortality data are limited.					
34003690	5	127	with	ng/mL	1149:1153	arg1	saturation					1174:1183	a transferrin saturation	1160:1183	a transferrin saturation <20%	1160:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	128	theme	Heart	632:636	arg1	Failure					638:644	Heart Failure	632:644	Heart Failure	632:644	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	5	129	theme	<20	1185:1187	arg1	%					1188:1188	%	1188:1188	%	1188:1188	Eligible patients are aged ≥18 years in stable chronic HF with New York Heart Association functional class II to IV symptoms, ejection fraction ≤40%, ID (ferritin <100 ng/mL or ferritin 100-300 ng/mL with a transferrin saturation <20%), and documented HF hospitalization or elevated N-terminal pro-brain natriuretic peptide.					
34003690	3	130	dep	trial	600:604	arg1	Carboxymaltose					614:627	Ferric Carboxymaltose	607:627	Ferric Carboxymaltose in Heart Failure With Iron Deficiency	607:665	METHODS The objective of the HEART-FID trial (Ferric Carboxymaltose in Heart Failure With Iron Deficiency) is to assess efficacy and safety of ferric carboxymaltose compared with placebo as treatment for symptomatic HF with reduced ejection fraction with ID.					
34003690	6	131	theme	additional	1413:1422	arg1	drug					1430:1433	additional study drug	1413:1433	additional study drug based on hemoglobin and iron indices for the trial duration	1413:1493	Consented patients are assigned to ferric carboxymaltose or placebo at baseline, with repeated visits/assessments every 6 months for additional study drug based on hemoglobin and iron indices for the trial duration.					
34003690	1	132	theme	heart	204:208	arg1	HF					219:220	HF	219:220	HF	219:220	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34003690	1	132	theme	heart	204:208	arg1	failure					210:216	heart failure	204:216	heart failure (HF)	204:221	BACKGROUND Iron deficiency (ID) has a prevalence of ≈40% to 50% among patients in heart failure (HF) with reduced ejection fraction and is associated with worse prognosis.					
34126148	0	0	theme	hybrid	96:101	arg1	nanomaterials					103:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	Fabrication of self-healing nanocomposite hydrogels with the cellulose nanocrystals-based Janus hybrid nanomaterials.					
34126148	4	1	theme	chain	602:606	arg1	extension					579:587	The extension	575:587	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating	575:662	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	7	2	theme	diverse	1172:1178	arg1	application					1180:1190	diverse application	1172:1190	diverse application	1172:1190	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	1	3	theme	series	186:191	arg1	design					174:179	the design	170:179	the design of a series of smart materials with unique asymmetric properties	170:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	4	4	theme	molecular	592:600	arg1	chain					602:606	molecular chain	592:606	molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating	592:662	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	7	5	contain	have	1167:1170	arg1	nanocomposites					1136:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	7	5	contain	have	1167:1170	arg2	application					1180:1190	diverse application	1172:1190	diverse application	1172:1190	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	5	6	theme	prepared	721:728	arg1	nanocomposites					730:743	the prepared nanocomposites	717:743	the prepared nanocomposites	717:743	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	6	7	theme	collaborative	845:857	arg1	effect					859:864	The collaborative effect	841:864	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).	841:1088	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	7	8	theme	self-healing	1235:1246	arg1	ability					1248:1254	self-healing ability	1235:1254	self-healing ability	1235:1254	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	6	9	theme	coating	935:941	arg1	chain					917:921	the molecular chain	903:921	the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%)	903:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	2	10	theme	hydrogels	447:455	arg1	construction					404:415	the construction	400:415	the construction of self-healing nanocomposite hydrogels	400:455	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	3	11	theme	mussel-inspired	503:517	arg1	chemistry					519:527	the mussel-inspired chemistry	499:527	the mussel-inspired chemistry	499:527	During emulsification, the mussel-inspired chemistry was employed to develop Janus nanocomposites.					
34126148	6	12	theme	PDA	931:933	arg1	coating					935:941	PDA coating	931:941	PDA coating	931:941	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	13	theme	molecular	907:915	arg1	chain					917:921	the molecular chain	903:921	the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%)	903:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	2	14	theme	nanocrystalline-type	300:319	arg1	nanomaterials					333:345	surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials	261:345	surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials	261:345	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	1	15	theme	smart	196:200	arg1	materials					202:210	smart materials	196:210	smart materials with unique asymmetric properties	196:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	7	16	theme	materials	1220:1228	arg1	development					1199:1209	the development	1195:1209	the development of smart materials with self-healing ability	1195:1254	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	7	17	theme	Janus	1117:1121	arg1	nanocomposites					1136:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	5	18	theme	-based	784:789	arg1	hydrogels					791:799	poly (acrylic acid) (PAA)-based hydrogels	759:799	poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization	759:838	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	6	19	with	endowed	970:976	arg1	8.8 MPa					1044:1050	8.8 MPa	1044:1050	8.8 MPa	1044:1050	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	19	with	endowed	970:976	arg1	properties					1032:1041	excellent mechanical properties	1011:1041	excellent mechanical properties (8.8 MPa)	1011:1051	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	19	with	endowed	970:976	arg1	efficiency					1070:1079	self-healing efficiency	1057:1079	self-healing efficiency (88.9%)	1057:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	19	with	endowed	970:976	arg1	%					1086:1086	88.9%	1082:1086	88.9%	1082:1086	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	1	20	theme	materials	202:210	arg1	series					186:191	a series	184:191	a series of smart materials with unique asymmetric properties	184:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	4	21	dep	poly-lysine	611:621	arg1	coating					656:662	coating	656:662	coating	656:662	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	6	22	theme	mechanical	1021:1030	arg1	8.8 MPa					1044:1050	8.8 MPa	1044:1050	8.8 MPa	1044:1050	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	22	theme	mechanical	1021:1030	arg1	properties					1032:1041	excellent mechanical properties	1011:1041	excellent mechanical properties (8.8 MPa)	1011:1051	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	7	23	theme	CNCs-PDA/PLL	1123:1134	arg1	nanocomposites					1136:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	1	24	theme	Janus	118:122	arg1	nanomaterials					124:136	Janus nanomaterials	118:136	Janus nanomaterials	118:136	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	2	25	theme	nanocomposite	433:445	arg1	hydrogels					447:455	self-healing nanocomposite hydrogels	420:455	self-healing nanocomposite hydrogels	420:455	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	0	26	theme	nanocomposite	28:40	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of self-healing nanocomposite	0:40	Fabrication of self-healing nanocomposite hydrogels with the cellulose nanocrystals-based Janus hybrid nanomaterials.					
34126148	6	27	theme	excellent	1011:1019	arg1	8.8 MPa					1044:1050	8.8 MPa	1044:1050	8.8 MPa	1044:1050	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	27	theme	excellent	1011:1019	arg1	properties					1032:1041	excellent mechanical properties	1011:1041	excellent mechanical properties (8.8 MPa)	1011:1051	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	1	28	from	prospects	157:165	arg1	design					174:179	the design	170:179	the design of a series of smart materials with unique asymmetric properties	170:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	7	29	with	materials	1220:1228	arg1	ability					1248:1254	self-healing ability	1235:1254	self-healing ability	1235:1254	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	1	30	theme	unique	217:222	arg1	properties					235:244	unique asymmetric properties	217:244	unique asymmetric properties	217:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	0	31	theme	self-healing	15:26	arg1	nanocomposite					28:40	self-healing nanocomposite	15:40	self-healing nanocomposite	15:40	Fabrication of self-healing nanocomposite hydrogels with the cellulose nanocrystals-based Janus hybrid nanomaterials.					
34126148	4	32	theme	polydopamine	637:648	arg1	chain					602:606	molecular chain	592:606	molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating	592:662	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	7	33	theme	synthesized	1105:1115	arg1	nanocomposites					1136:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	the synthesized Janus CNCs-PDA/PLL nanocomposites	1101:1149	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
34126148	6	34	theme	PLL	926:928	arg1	chain					917:921	the molecular chain	903:921	the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%)	903:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	1	35	theme	asymmetric	224:233	arg1	properties					235:244	unique asymmetric properties	217:244	unique asymmetric properties	217:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	5	36	theme	acrylic	765:771	arg1	poly					759:762	poly	759:762	poly (acrylic acid)	759:777	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	5	36	theme	acrylic	765:771	arg1	acid					773:776	acrylic acid	765:776	acrylic acid	765:776	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	6	37	dep	ions	965:968	arg1	endowed					970:976	endowed	970:976	endowed	970:976	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	5	38	theme	in-situ	817:823	arg1	polymerization					825:838	in-situ polymerization	817:838	in-situ polymerization	817:838	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	1	39	contain	possess	138:144	arg1	nanomaterials					124:136	Janus nanomaterials	118:136	Janus nanomaterials	118:136	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	1	39	contain	possess	138:144	arg2	prospects					157:165	remarkable prospects	146:165	remarkable prospects in the design of a series of smart materials with unique asymmetric properties	146:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	6	40	theme	self-healing	1057:1068	arg1	efficiency					1070:1079	self-healing efficiency	1057:1079	self-healing efficiency (88.9%)	1057:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	40	theme	self-healing	1057:1068	arg1	%					1086:1086	88.9%	1082:1086	88.9%	1082:1086	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	4	41	theme	CNCs	699:702	arg1	sides					690:694	different sides	680:694	different sides of CNCs	680:702	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	0	42	theme	cellulose	61:69	arg1	nanomaterials					103:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	Fabrication of self-healing nanocomposite hydrogels with the cellulose nanocrystals-based Janus hybrid nanomaterials.					
34126148	4	43	theme	poly-lysine	611:621	arg1	chain					602:606	molecular chain	592:606	molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating	592:662	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	1	44	with	materials	202:210	arg1	properties					235:244	unique asymmetric properties	217:244	unique asymmetric properties	217:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	5	45	dep	-based	784:789	arg1	poly					759:762	poly	759:762	poly (acrylic acid)	759:777	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	5	45	dep	-based	784:789	arg1	acid					773:776	acrylic acid	765:776	acrylic acid	765:776	Afterwards, the prepared nanocomposites were added to poly (acrylic acid) (PAA)-based hydrogels which formed by in-situ polymerization.					
34126148	1	46	theme	remarkable	146:155	arg1	prospects					157:165	remarkable prospects	146:165	remarkable prospects in the design of a series of smart materials with unique asymmetric properties	146:244	Janus nanomaterials possess remarkable prospects in the design of a series of smart materials with unique asymmetric properties.					
34126148	6	47	theme	PAA	944:946	arg1	chains					948:953	PAA chains	944:953	PAA chains	944:953	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	48	theme	chains	948:953	arg1	chain					917:921	the molecular chain	903:921	the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%)	903:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	49	theme	ions	965:968	arg1	chain					917:921	the molecular chain	903:921	the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%)	903:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	6	50	theme	nanocomposite	982:994	arg1	hydrogels					996:1004	the nanocomposite hydrogels	978:1004	the nanocomposite hydrogels	978:1004	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	2	51	theme	cellulose	290:298	arg1	CNCs-type					322:330	CNCs-type	322:330	CNCs-type	322:330	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	51	theme	cellulose	290:298	arg1	nanocrystalline-type					300:319	surface functionalized Janus cellulose nanocrystalline-type	261:319	surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials	261:345	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	52	theme	emulsion	378:385	arg1	template					387:394	Pickering emulsion template	368:394	Pickering emulsion template	368:394	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	6	53	theme	coordination	882:893	arg1	effect					859:864	The collaborative effect	841:864	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).	841:1088	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	2	54	theme	Janus	284:288	arg1	CNCs-type					322:330	CNCs-type	322:330	CNCs-type	322:330	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	54	theme	Janus	284:288	arg1	nanocrystalline-type					300:319	surface functionalized Janus cellulose nanocrystalline-type	261:319	surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials	261:345	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	55	theme	Pickering	368:376	arg1	template					387:394	Pickering emulsion template	368:394	Pickering emulsion template	368:394	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	3	56	theme	Janus	553:557	arg1	nanocomposites					559:572	Janus nanocomposites	553:572	Janus nanocomposites	553:572	During emulsification, the mussel-inspired chemistry was employed to develop Janus nanocomposites.					
34126148	0	57	theme	Janus	90:94	arg1	nanomaterials					103:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	Fabrication of self-healing nanocomposite hydrogels with the cellulose nanocrystals-based Janus hybrid nanomaterials.					
34126148	6	58	theme	metal-ligand	869:880	arg1	coordination					882:893	metal-ligand coordination	869:893	metal-ligand coordination	869:893	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	2	59	theme	functionalized	269:282	arg1	CNCs-type					322:330	CNCs-type	322:330	CNCs-type	322:330	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	59	theme	functionalized	269:282	arg1	nanocrystalline-type					300:319	surface functionalized Janus cellulose nanocrystalline-type	261:319	surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials	261:345	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	60	theme	self-healing	420:431	arg1	hydrogels					447:455	self-healing nanocomposite hydrogels	420:455	self-healing nanocomposite hydrogels	420:455	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	4	61	theme	different	680:688	arg1	sides					690:694	different sides	680:694	different sides of CNCs	680:702	The extension of molecular chain of poly-lysine (PLL) and the polydopamine (PDA) coating were grafted on different sides of CNCs.					
34126148	0	62	theme	nanocrystals-based	71:88	arg1	nanomaterials					103:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	the cellulose nanocrystals-based Janus hybrid nanomaterials	57:115	Fabrication of self-healing nanocomposite hydrogels with the cellulose nanocrystals-based Janus hybrid nanomaterials.					
34126148	6	63	theme	metal	959:963	arg1	ions					965:968	metal ions	959:968	metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%)	959:1087	The collaborative effect of metal-ligand coordination between the molecular chain of PLL, PDA coating, PAA chains and metal ions endowed the nanocomposite hydrogels with excellent mechanical properties (8.8 MPa) and self-healing efficiency (88.9%).					
34126148	2	64	theme	surface	261:267	arg1	CNCs-type					322:330	CNCs-type	322:330	CNCs-type	322:330	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	2	64	theme	surface	261:267	arg1	nanocrystalline-type					300:319	surface functionalized Janus cellulose nanocrystalline-type	261:319	surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials	261:345	In this work, surface functionalized Janus cellulose nanocrystalline-type (CNCs-type) nanomaterials were manufactured by Pickering emulsion template and the construction of self-healing nanocomposite hydrogels has been realized.					
34126148	7	65	theme	smart	1214:1218	arg1	materials					1220:1228	smart materials	1214:1228	smart materials with self-healing ability	1214:1254	Therefore, the synthesized Janus CNCs-PDA/PLL nanocomposites are expected to have diverse application in the development of smart materials with self-healing ability.					
33632232	0	0	theme	breast	92:97	arg1	cancer					99:104	triple negative breast cancer	76:104	triple negative breast cancer	76:104	A paclitaxel and microRNA-124 coloaded stepped cleavable nanosystem against triple negative breast cancer.					
33632232	6	1	theme	tumour	1214:1219	arg1	metastasis					1221:1230	tumour metastasis	1214:1230	tumour metastasis	1214:1230	Inside tumour cells, o-HA detached from CaP/LNS due to the reduction of disulfide bonds by glutathione (GSH) and inhibited tumour metastasis.					
33632232	2	2	theme	advanced	351:358	arg1	TNBC					360:363	advanced TNBC	351:363	advanced TNBC	351:363	Therefore, studies aiming to explore new therapeutic strategies for advanced TNBC are urgently needed.					
33632232	0	3	theme	negative	83:90	arg1	cancer					99:104	triple negative breast cancer	76:104	triple negative breast cancer	76:104	A paclitaxel and microRNA-124 coloaded stepped cleavable nanosystem against triple negative breast cancer.					
33632232	5	4	theme	tumor	1078:1082	arg1	cells					1084:1088	tumor cells	1078:1088	tumor cells	1078:1088	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	9	5	theme	miR124	1606:1611	arg1	codelivery					1584:1593	the codelivery	1580:1593	the codelivery of PTX and miR124 by the CaP/LNS nanosystem	1580:1637	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	9	5	theme	miR124	1606:1611	arg1	strategy					1672:1679	a promising therapeutic strategy	1648:1679	a promising therapeutic strategy for TNBC	1648:1688	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	5	6	theme	surface	863:869	arg1	layer					875:879	the surface PEG layer	859:879	the surface PEG layer	859:879	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	7	theme	PEG	871:873	arg1	layer					875:879	the surface PEG layer	859:879	the surface PEG layer	859:879	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	8	8	theme	significant	1454:1464	arg1	efficiency					1477:1486	significant antitumour efficiency	1454:1486	significant antitumour efficiency	1454:1486	Moreover, PTX/miR124-NP showed significant antitumour efficiency and excellent safety in mice bearing MDA-MB-231 tumours.					
33632232	5	9	theme	cells	1084:1088	arg1	surface					1067:1073	the surface	1063:1073	the surface of tumor cells	1063:1088	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	6	10	theme	tumour	1098:1103	arg1	cells					1105:1109	tumour cells	1098:1109	tumour cells	1098:1109	Inside tumour cells, o-HA detached from CaP/LNS due to the reduction of disulfide bonds by glutathione (GSH) and inhibited tumour metastasis.					
33632232	5	11	theme	oligomeric	952:961	arg1	o-HA					980:983	o-HA	980:983	o-HA	980:983	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	11	theme	oligomeric	952:961	arg1	acid					974:977	exposed oligomeric hyaluronic acid	944:977	exposed oligomeric hyaluronic acid (o-HA)	944:984	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	12	theme	CD44	1046:1049	arg1	receptor					1051:1058	the CD44 receptor	1042:1058	the CD44 receptor on the surface of tumor cells	1042:1088	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	13	theme	tumor	926:930	arg1	tissues					932:938	tumor tissues	926:938	tumor tissues	926:938	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	4	14	theme	phosphate	682:690	arg1	nanosystem					708:717	a stepped cleavable calcium phosphate composite lipid nanosystem	654:717	a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS)	654:727	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	14	theme	phosphate	682:690	arg1	CaP/LNS					720:726	CaP/LNS	720:726	CaP/LNS	720:726	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	7	15	attach	released	1272:1279	arg2	miR124					1247:1252	miR124	1247:1252	miR124	1247:1252	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	7	15	attach	released	1272:1279	arg1	CaP/LNS					1286:1292	CaP/LNS	1286:1292	CaP/LNS	1286:1292	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	7	15	attach	released	1272:1279	arg2	PTX					1239:1241	PTX	1239:1241	PTX	1239:1241	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	4	16	theme	cleavable	664:672	arg1	nanosystem					708:717	a stepped cleavable calcium phosphate composite lipid nanosystem	654:717	a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS)	654:727	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	16	theme	cleavable	664:672	arg1	CaP/LNS					720:726	CaP/LNS	720:726	CaP/LNS	720:726	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	5	17	theme	superior	798:805	arg1	microenvironment					813:828	superior tumor microenvironment	798:828	superior tumor microenvironment responsive ability	798:847	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	3	18	theme	tumour	455:460	arg1	growth					462:467	tumour growth	455:467	tumour growth	455:467	According to recent studies, microRNA-124 (miR124) not only inhibits tumour growth but also increases the sensitivity of TNBC to paclitaxel (PTX), suggesting that a platform combining PTX and miR124 may be an advanced solution for TNBC.					
33632232	5	19	theme	tissues	932:938	arg1	environment					911:921	the mildly acidic environment	893:921	the mildly acidic environment of tumor tissues	893:938	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	20	theme	responsive	830:839	arg1	ability					841:847	superior tumor microenvironment responsive ability	798:847	superior tumor microenvironment responsive ability	798:847	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	0	21	theme	cleavable	47:55	arg1	nanosystem					57:66	stepped cleavable nanosystem	39:66	stepped cleavable nanosystem	39:66	A paclitaxel and microRNA-124 coloaded stepped cleavable nanosystem against triple negative breast cancer.					
33632232	7	22	theme	Transition	1377:1386	arg1	process					1394:1400	the Epithelial-Mesenchymal Transition (EMT) process	1350:1400	the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells	1350:1420	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	8	23	theme	excellent	1492:1500	arg1	safety					1502:1507	excellent safety	1492:1507	excellent safety	1492:1507	Moreover, PTX/miR124-NP showed significant antitumour efficiency and excellent safety in mice bearing MDA-MB-231 tumours.					
33632232	9	24	theme	promising	1650:1658	arg1	codelivery					1584:1593	the codelivery	1580:1593	the codelivery of PTX and miR124 by the CaP/LNS nanosystem	1580:1637	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	9	24	theme	promising	1650:1658	arg1	strategy					1672:1679	a promising therapeutic strategy	1648:1679	a promising therapeutic strategy for TNBC	1648:1688	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	4	25	theme	lipid	702:706	arg1	nanosystem					708:717	a stepped cleavable calcium phosphate composite lipid nanosystem	654:717	a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS)	654:727	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	25	theme	lipid	702:706	arg1	CaP/LNS					720:726	CaP/LNS	720:726	CaP/LNS	720:726	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	7	26	theme	MDA-MB-231	1405:1414	arg1	cells					1416:1420	MDA-MB-231 cells	1405:1420	MDA-MB-231 cells	1405:1420	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	7	27	from	process	1394:1400	arg1	cells					1416:1420	MDA-MB-231 cells	1405:1420	MDA-MB-231 cells	1405:1420	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	1	28	theme	poor	267:270	arg1	prognosis					272:280	a poor prognosis	265:280	a poor prognosis	265:280	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	4	29	theme	composite	692:700	arg1	nanosystem					708:717	a stepped cleavable calcium phosphate composite lipid nanosystem	654:717	a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS)	654:727	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	29	theme	composite	692:700	arg1	CaP/LNS					720:726	CaP/LNS	720:726	CaP/LNS	720:726	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	1	30	theme	BACKGROUND	107:116	arg1	cancer					141:146	BACKGROUND Triple negative breast cancer	107:146	BACKGROUND Triple negative breast cancer (TNBC)	107:153	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	1	30	theme	BACKGROUND	107:116	arg1	TNBC					149:152	TNBC	149:152	TNBC	149:152	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	7	31	theme	synergistic	1306:1316	arg1	effects					1329:1335	synergistic antitumour effects	1306:1335	synergistic antitumour effects	1306:1335	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	1	32	theme	aggressive	187:196	arg1	cancers					205:211	the most biologically aggressive breast cancers	165:211	the most biologically aggressive breast cancers	165:211	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	5	33	from	receptor	1051:1058	arg1	surface					1067:1073	the surface	1063:1073	the surface of tumor cells	1063:1088	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	8	34	theme	MDA-MB-231	1525:1534	arg1	tumours					1536:1542	MDA-MB-231 tumours	1525:1542	MDA-MB-231 tumours	1525:1542	Moreover, PTX/miR124-NP showed significant antitumour efficiency and excellent safety in mice bearing MDA-MB-231 tumours.					
33632232	7	35	theme	Epithelial-Mesenchymal	1354:1375	arg1	Transition					1377:1386	Epithelial-Mesenchymal Transition	1354:1386	the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells	1350:1420	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	7	35	theme	Epithelial-Mesenchymal	1354:1375	arg1	EMT					1389:1391	EMT	1389:1391	EMT	1389:1391	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	5	36	theme	CaP/LNS	1021:1027	arg1	uptake					1011:1016	the cellular uptake	998:1016	the cellular uptake of CaP/LNS	998:1027	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	1	37	theme	Triple	118:123	arg1	cancer					141:146	BACKGROUND Triple negative breast cancer	107:146	BACKGROUND Triple negative breast cancer (TNBC)	107:153	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	1	37	theme	Triple	118:123	arg1	TNBC					149:152	TNBC	149:152	TNBC	149:152	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	1	38	theme	breast	198:203	arg1	cancers					205:211	the most biologically aggressive breast cancers	165:211	the most biologically aggressive breast cancers	165:211	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	0	39	theme	stepped	39:45	arg1	nanosystem					57:66	stepped cleavable nanosystem	39:66	stepped cleavable nanosystem	39:66	A paclitaxel and microRNA-124 coloaded stepped cleavable nanosystem against triple negative breast cancer.					
33632232	2	40	theme	therapeutic	324:334	arg1	strategies					336:345	new therapeutic strategies	320:345	new therapeutic strategies for advanced TNBC	320:363	Therefore, studies aiming to explore new therapeutic strategies for advanced TNBC are urgently needed.					
33632232	9	41	theme	therapeutic	1660:1670	arg1	codelivery					1584:1593	the codelivery	1580:1593	the codelivery of PTX and miR124 by the CaP/LNS nanosystem	1580:1637	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	9	41	theme	therapeutic	1660:1670	arg1	strategy					1672:1679	a promising therapeutic strategy	1648:1679	a promising therapeutic strategy for TNBC	1648:1688	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	5	42	theme	tumor	807:811	arg1	microenvironment					813:828	superior tumor microenvironment	798:828	superior tumor microenvironment responsive ability	798:847	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	6	43	theme	bonds	1173:1177	arg1	reduction					1150:1158	the reduction	1146:1158	the reduction of disulfide bonds by glutathione (GSH)	1146:1198	Inside tumour cells, o-HA detached from CaP/LNS due to the reduction of disulfide bonds by glutathione (GSH) and inhibited tumour metastasis.					
33632232	1	44	theme	cancers	205:211	arg1	cancers					205:211	the most biologically aggressive breast cancers	165:211	the most biologically aggressive breast cancers	165:211	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	1	44	theme	cancers	205:211	arg1	one					158:160	one	158:160	one	158:160	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	6	45	theme	disulfide	1163:1171	arg1	bonds					1173:1177	disulfide bonds	1163:1177	disulfide bonds	1163:1177	Inside tumour cells, o-HA detached from CaP/LNS due to the reduction of disulfide bonds by glutathione (GSH) and inhibited tumour metastasis.					
33632232	5	46	theme	microenvironment	813:828	arg1	ability					841:847	superior tumor microenvironment responsive ability	798:847	superior tumor microenvironment responsive ability	798:847	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	3	47	theme	advanced	595:602	arg1	solution					604:611	an advanced solution	592:611	an advanced solution for TNBC	592:620	According to recent studies, microRNA-124 (miR124) not only inhibits tumour growth but also increases the sensitivity of TNBC to paclitaxel (PTX), suggesting that a platform combining PTX and miR124 may be an advanced solution for TNBC.					
33632232	3	47	theme	advanced	595:602	arg1	platform					551:558	a platform	549:558	a platform combining PTX and miR124	549:583	According to recent studies, microRNA-124 (miR124) not only inhibits tumour growth but also increases the sensitivity of TNBC to paclitaxel (PTX), suggesting that a platform combining PTX and miR124 may be an advanced solution for TNBC.					
33632232	1	48	theme	negative	125:132	arg1	cancer					141:146	BACKGROUND Triple negative breast cancer	107:146	BACKGROUND Triple negative breast cancer (TNBC)	107:153	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	1	48	theme	negative	125:132	arg1	TNBC					149:152	TNBC	149:152	TNBC	149:152	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	3	49	theme	recent	399:404	arg1	studies					406:412	recent studies	399:412	recent studies	399:412	According to recent studies, microRNA-124 (miR124) not only inhibits tumour growth but also increases the sensitivity of TNBC to paclitaxel (PTX), suggesting that a platform combining PTX and miR124 may be an advanced solution for TNBC.					
33632232	1	50	theme	breast	134:139	arg1	cancer					141:146	BACKGROUND Triple negative breast cancer	107:146	BACKGROUND Triple negative breast cancer (TNBC)	107:153	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	1	50	theme	breast	134:139	arg1	TNBC					149:152	TNBC	149:152	TNBC	149:152	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	5	51	theme	cellular	1002:1009	arg1	uptake					1011:1016	the cellular uptake	998:1016	the cellular uptake of CaP/LNS	998:1027	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	52	theme	exposed	944:950	arg1	o-HA					980:983	o-HA	980:983	o-HA	980:983	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	52	theme	exposed	944:950	arg1	acid					974:977	exposed oligomeric hyaluronic acid	944:977	exposed oligomeric hyaluronic acid (o-HA)	944:984	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	7	53	theme	antitumour	1318:1327	arg1	effects					1329:1335	synergistic antitumour effects	1306:1335	synergistic antitumour effects	1306:1335	Then, PTX and miR124 were sequentially released from CaP/LNS and exerted synergistic antitumour effects by reversing the Epithelial-Mesenchymal Transition (EMT) process in MDA-MB-231 cells.					
33632232	2	54	theme	new	320:322	arg1	strategies					336:345	new therapeutic strategies	320:345	new therapeutic strategies for advanced TNBC	320:363	Therefore, studies aiming to explore new therapeutic strategies for advanced TNBC are urgently needed.					
33632232	5	55	theme	acidic	904:909	arg1	environment					911:921	the mildly acidic environment	893:921	the mildly acidic environment of tumor tissues	893:938	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	4	56	dep	RESULTS	623:629	arg1	constructed					642:652	constructed	642:652	constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP)	642:771	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	57	theme	calcium	674:680	arg1	nanosystem					708:717	a stepped cleavable calcium phosphate composite lipid nanosystem	654:717	a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS)	654:727	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	57	theme	calcium	674:680	arg1	CaP/LNS					720:726	CaP/LNS	720:726	CaP/LNS	720:726	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	1	58	theme	effective	223:231	arg1	options					243:249	effective treatment options	223:249	effective treatment options	223:249	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	0	59	theme	triple	76:81	arg1	cancer					99:104	triple negative breast cancer	76:104	triple negative breast cancer	76:104	A paclitaxel and microRNA-124 coloaded stepped cleavable nanosystem against triple negative breast cancer.					
33632232	5	60	theme	hyaluronic	963:972	arg1	o-HA					980:983	o-HA	980:983	o-HA	980:983	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	5	60	theme	hyaluronic	963:972	arg1	acid					974:977	exposed oligomeric hyaluronic acid	944:977	exposed oligomeric hyaluronic acid (o-HA)	944:984	PTX/miR124-NP exhibited superior tumor microenvironment responsive ability, in which the surface PEG layer was shed in the mildly acidic environment of tumor tissues and exposed oligomeric hyaluronic acid (o-HA) facilitated the cellular uptake of CaP/LNS by targeting the CD44 receptor on the surface of tumor cells.					
33632232	1	61	theme	treatment	233:241	arg1	options					243:249	effective treatment options	223:249	effective treatment options	223:249	BACKGROUND Triple negative breast cancer (TNBC) is one of the most biologically aggressive breast cancers and lacks effective treatment options, resulting in a poor prognosis.					
33632232	9	62	theme	CaP/LNS	1620:1626	arg1	nanosystem					1628:1637	the CaP/LNS nanosystem	1616:1637	the CaP/LNS nanosystem	1616:1637	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	9	63	theme	PTX	1598:1600	arg1	codelivery					1584:1593	the codelivery	1580:1593	the codelivery of PTX and miR124 by the CaP/LNS nanosystem	1580:1637	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	9	63	theme	PTX	1598:1600	arg1	strategy					1672:1679	a promising therapeutic strategy	1648:1679	a promising therapeutic strategy for TNBC	1648:1688	CONCLUSION Based on these results, the codelivery of PTX and miR124 by the CaP/LNS nanosystem might be a promising therapeutic strategy for TNBC.					
33632232	3	64	theme	TNBC	507:510	arg1	sensitivity					492:502	the sensitivity	488:502	the sensitivity of TNBC to paclitaxel (PTX)	488:530	According to recent studies, microRNA-124 (miR124) not only inhibits tumour growth but also increases the sensitivity of TNBC to paclitaxel (PTX), suggesting that a platform combining PTX and miR124 may be an advanced solution for TNBC.					
33632232	8	65	theme	antitumour	1466:1475	arg1	efficiency					1477:1486	significant antitumour efficiency	1454:1486	significant antitumour efficiency	1454:1486	Moreover, PTX/miR124-NP showed significant antitumour efficiency and excellent safety in mice bearing MDA-MB-231 tumours.					
33632232	4	66	theme	stepped	656:662	arg1	nanosystem					708:717	a stepped cleavable calcium phosphate composite lipid nanosystem	654:717	a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS)	654:727	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
33632232	4	66	theme	stepped	656:662	arg1	CaP/LNS					720:726	CaP/LNS	720:726	CaP/LNS	720:726	RESULTS Herein, we constructed a stepped cleavable calcium phosphate composite lipid nanosystem (CaP/LNS) to codeliver PTX and miR124 (PTX/miR124-NP).					
35127162	7	0	theme	Akkermansia	1531:1541	arg1	abundance					1503:1511	abundance	1503:1511	abundance of Lactobacillus, Akkermansia, and Bifidobacterium	1503:1562	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	8	1	theme	SCFAs	1691:1695	arg1	concentration					1674:1686	significantly increased concentration	1650:1686	significantly increased concentration of SCFAs in fecal samples	1650:1712	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	1	2	theme	few	290:292	arg1	studies					294:300	few studies	290:300	few studies	290:300	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	7	3	theme	Lactobacillus	1516:1528	arg1	abundance					1503:1511	abundance	1503:1511	abundance of Lactobacillus, Akkermansia, and Bifidobacterium	1503:1562	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	4	4	theme	mouse	924:928	arg1	tissues					936:942	the mouse colon tissues	920:942	the mouse colon tissues 24 h after LPS administration	920:972	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	2	5	from	Objectives	353:362	arg1	study					372:376	this study	367:376	this study	367:376	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	7	6	theme	FTJS7K1	1458:1464	arg1	group					1466:1470	the B. vulgatus FTJS7K1 group	1442:1470	the B. vulgatus FTJS7K1 group	1442:1470	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	10	7	theme	gut	2117:2119	arg1	microbiota					2121:2130	the gut microbiota	2113:2130	the gut microbiota	2113:2130	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	8	8	theme	fecal	1700:1704	arg1	samples					1706:1712	fecal samples	1700:1712	fecal samples	1700:1712	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	10	9	theme	vulgatus	1990:1997	arg1	FTJS7K1					1999:2005	B. vulgatus FTJS7K1	1987:2005	B. vulgatus FTJS7K1	1987:2005	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	6	10	theme	colon	1324:1328	arg1	tissues					1330:1336	the colon tissues	1320:1336	the colon tissues	1320:1336	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	4	11	theme	cytokine	884:891	arg1	expression					893:902	cytokine expression	884:902	cytokine expression	884:902	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	7	12	theme	B.	1446:1447	arg1	group					1466:1470	the B. vulgatus FTJS7K1 group	1442:1470	the B. vulgatus FTJS7K1 group	1442:1470	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	11	13	theme	next-generation	2210:2224	arg1	probiotics					2226:2235	next-generation probiotics	2210:2235	next-generation probiotics	2210:2235	These results provide support for the screening of the Bacteroides genus for next-generation probiotics.					
35127162	5	14	from	effects	1045:1051	arg1	parameters					1069:1078	the assessed parameters	1056:1078	the assessed parameters	1056:1078	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	9	15	theme	analysis	1738:1745	arg1	results					1719:1725	The results	1715:1725	The results of genomic analysis	1715:1745	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	9	16	theme	B.	1785:1786	arg1	FTJS7K1					1797:1803	B. vulgatus FTJS7K1	1785:1803	B. vulgatus FTJS7K1	1785:1803	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	3	17	theme	intestinal	785:794	arg1	injury					796:801	LPS-induced acute intestinal injury	767:801	LPS-induced acute intestinal injury in C57BL/6J mice	767:818	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	6	18	theme	mRNA	1301:1304	arg1	expression					1306:1315	cytokine mRNA expression	1292:1315	cytokine mRNA expression	1292:1315	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	4	19	dep	Results	821:827	arg1	observed					908:915	observed	908:915	was observed in the mouse colon tissues 24 h after LPS administration	904:972	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	7	20	theme	LPS	1431:1433	arg1	group					1435:1439	the LPS group	1427:1439	the LPS group	1427:1439	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	3	21	theme	LPS-induced	767:777	arg1	injury					796:801	LPS-induced acute intestinal injury	767:801	LPS-induced acute intestinal injury in C57BL/6J mice	767:818	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	6	22	theme	cells	1377:1381	arg1	proportion					1350:1359	the proportion	1346:1359	the proportion of regulatory T cells in the mesenteric lymph node	1346:1410	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	1	23	theme	intestinal	238:247	arg1	injury					249:254	intestinal injury	238:254	intestinal injury	238:254	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	2	24	theme	C57BL/6J	556:563	arg1	mice					565:568	C57BL/6J mice	556:568	C57BL/6J mice	556:568	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	6	25	theme	cytokine	1292:1299	arg1	expression					1306:1315	cytokine mRNA expression	1292:1315	cytokine mRNA expression	1292:1315	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	10	26	theme	cytokine	2038:2045	arg1	production					2047:2056	cytokine production	2038:2056	cytokine production in the colon tissue	2038:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	6	27	theme	regulatory	1364:1373	arg1	cells					1377:1381	regulatory T cells	1364:1381	regulatory T cells	1364:1381	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	3	28	theme	intestinal	621:630	arg1	permeability					643:654	intestinal epithelial permeability	621:654	intestinal epithelial permeability	621:654	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	4	29	from	change	874:879	arg1	expression					893:902	cytokine expression	884:902	cytokine expression	884:902	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	5	30	dep	showed	1028:1033	arg1	administration					1116:1129	only the administration	1107:1129	only the administration of B. vulgatus FTJS7K1	1107:1152	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	5	30	dep	showed	1028:1033	arg1	able					1158:1161	able	1158:1161	able	1158:1161	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	8	31	dep	B.	1612:1613	arg1	vulgatus					1615:1622	vulgatus	1615:1622	vulgatus	1615:1622	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	9	32	theme	vulgatus	1788:1795	arg1	FTJS7K1					1797:1803	B. vulgatus FTJS7K1	1785:1803	B. vulgatus FTJS7K1	1785:1803	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	3	33	from	Alterations	579:589	arg1	permeability					643:654	intestinal epithelial permeability	621:654	intestinal epithelial permeability	621:654	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	3	33	from	Alterations	579:589	arg1	concentration					703:715	short-chain fatty acid (SCFA) concentration	673:715	short-chain fatty acid (SCFA) concentration	673:715	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	3	33	from	Alterations	579:589	arg1	level					666:670	cytokine level	657:670	cytokine level	657:670	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	3	33	from	Alterations	579:589	arg1	microbiota					609:618	the intestinal microbiota	594:618	the intestinal microbiota	594:618	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	3	33	from	Alterations	579:589	arg1	responses					729:737	immune responses	722:737	immune responses	722:737	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	10	34	theme	colon	2065:2069	arg1	tissue					2071:2076	the colon tissue	2061:2076	the colon tissue	2061:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	3	35	theme	cytokine	657:664	arg1	level					666:670	cytokine level	657:670	cytokine level	657:670	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	4	36	from	damage	849:854	arg1	expression					893:902	cytokine expression	884:902	cytokine expression	884:902	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	5	37	theme	Oral	975:978	arg1	administration					980:993	Oral administration	975:993	Oral administration of different B. vulgatus strains	975:1026	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	0	38	theme	lipopolysaccharide-induced	69:94	arg1	injury					113:118	lipopolysaccharide-induced acute intestinal injury	69:118	lipopolysaccharide-induced acute intestinal injury	69:118	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	0	39	theme	intestinal	102:111	arg1	injury					113:118	lipopolysaccharide-induced acute intestinal injury	69:118	lipopolysaccharide-induced acute intestinal injury	69:118	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	1	40	theme	Bacteroides	186:196	arg1	species					198:204	Bacteroides species	186:204	Bacteroides species	186:204	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	9	41	theme	specific	1829:1836	arg1	genes					1838:1842	specific genes	1829:1842	specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs)	1829:1923	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	6	42	theme	lymph	1401:1405	arg1	node					1407:1410	the mesenteric lymph node	1386:1410	the mesenteric lymph node	1386:1410	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	5	43	theme	vulgatus	1137:1144	arg1	FTJS7K1					1146:1152	B. vulgatus FTJS7K1	1134:1152	B. vulgatus FTJS7K1	1134:1152	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	9	44	dep	secretion	1905:1913	arg1	e.g.					1895:1898	e.g.	1895:1898	e.g.	1895:1898	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	5	45	theme	strains	1020:1026	arg1	administration					980:993	Oral administration	975:993	Oral administration of different B. vulgatus strains	975:1026	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	2	46	theme	acute	529:533	arg1	injury					546:551	lipopolysaccharide (LPS)-induced acute intestinal injury	496:551	lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice	496:568	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	5	47	theme	different	1035:1043	arg1	effects					1045:1051	different effects	1035:1051	different effects on the assessed parameters of the mice	1035:1090	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	9	48	theme	defense	1860:1866	arg1	mechanisms					1868:1877	defense mechanisms	1860:1877	defense mechanisms	1860:1877	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	0	49	theme	Protective	0:9	arg1	effects					11:17	Protective effects	0:17	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.	0:158	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	5	50	theme	epithelium	1220:1229	arg1	integrity					1192:1200	the architectural integrity	1174:1200	the architectural integrity of the intestinal epithelium	1174:1229	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	11	51	theme	Bacteroides	2188:2198	arg1	genus					2200:2204	the Bacteroides genus	2184:2204	the Bacteroides genus	2184:2204	These results provide support for the screening of the Bacteroides genus for next-generation probiotics.					
35127162	2	52	theme	genomic	434:440	arg1	characteristics					442:456	their genomic characteristics	428:456	their genomic characteristics	428:456	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	10	53	from	production	2047:2056	arg1	tissue					2071:2076	the colon tissue	2061:2076	the colon tissue	2061:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	5	54	theme	B.	1008:1009	arg1	strains					1020:1026	different B. vulgatus strains	998:1026	different B. vulgatus strains	998:1026	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	3	55	theme	fatty	685:689	arg1	SCFA					697:700	SCFA	697:700	SCFA	697:700	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	3	55	theme	fatty	685:689	arg1	acid					691:694	short-chain fatty acid	673:694	short-chain fatty acid (SCFA) concentration	673:715	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	0	56	theme	Bacteroides	32:42	arg1	strains					53:59	different Bacteroides vulgatus strains	22:59	different Bacteroides vulgatus strains	22:59	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	7	57	dep	showed	1472:1477	arg1	increased					1493:1501	increased	1493:1501	increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium	1493:1562	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	0	58	theme	strains	53:59	arg1	effects					11:17	Protective effects	0:17	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.	0:158	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	1	59	theme	vulgatus	343:350	arg1	roles					322:326	the roles	318:326	the roles of Bacteroides vulgatus	318:350	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	10	60	from	tissue	2071:2076	arg1	modulation					2024:2033	modulation	2024:2033	modulation of cytokine production in the colon tissue	2024:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	10	60	from	tissue	2071:2076	arg1	regulation					2082:2091	regulation	2082:2091	regulation of the structure of the gut microbiota	2082:2130	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	7	61	theme	Bifidobacterium	1548:1562	arg1	abundance					1503:1511	abundance	1503:1511	abundance of Lactobacillus, Akkermansia, and Bifidobacterium	1503:1562	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	5	62	theme	mice	1087:1090	arg1	effects					1045:1051	different effects	1035:1051	different effects on the assessed parameters of the mice	1035:1090	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	4	63	located	observed	908:915	arg2	change					874:879	a significant change	860:879	a significant change in cytokine expression	860:902	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	4	63	located	observed	908:915	arg2	damage					849:854	Severe histological damage	829:854	Severe histological damage	829:854	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	4	63	located	observed	908:915	arg1	tissues					936:942	the mouse colon tissues	920:942	the mouse colon tissues 24 h after LPS administration	920:972	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	4	64	theme	Severe	829:834	arg1	damage					849:854	Severe histological damage	829:854	Severe histological damage	829:854	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	10	65	from	regulation	2082:2091	arg1	tissue					2071:2076	the colon tissue	2061:2076	the colon tissue	2061:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	9	66	theme	SCFAs	1918:1922	arg1	secretion					1905:1913	the secretion	1901:1913	the secretion of SCFAs	1901:1922	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	2	67	theme	vulgatus	387:394	arg1	strains					396:402	four B. vulgatus strains	379:402	four B. vulgatus strains	379:402	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	2	67	theme	vulgatus	387:394	arg1	Objectives					353:362	Objectives	353:362	Objectives In this study	353:376	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	5	68	theme	assessed	1060:1067	arg1	parameters					1069:1078	the assessed parameters	1056:1078	the assessed parameters	1056:1078	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	8	69	theme	increased	1664:1672	arg1	concentration					1674:1686	significantly increased concentration	1650:1686	significantly increased concentration of SCFAs in fecal samples	1650:1712	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	3	70	theme	immune	722:727	arg1	responses					729:737	immune responses	722:737	immune responses	722:737	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	10	71	theme	microbiota	2121:2130	arg1	structure					2100:2108	the structure	2096:2108	the structure of the gut microbiota	2096:2130	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	6	72	theme	B.	1232:1233	arg1	FTJS7K1					1244:1250	B. vulgatus FTJS7K1	1232:1250	B. vulgatus FTJS7K1	1232:1250	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	10	73	theme	B.	1987:1988	arg1	FTJS7K1					1999:2005	B. vulgatus FTJS7K1	1987:2005	B. vulgatus FTJS7K1	1987:2005	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	1	74	theme	species	198:204	arg1	Introduction					160:171	Introduction The roles	160:181	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury	160:254	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	9	75	theme	genomic	1730:1736	arg1	analysis					1738:1745	genomic analysis	1730:1745	genomic analysis	1730:1745	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	1	76	theme	alleviating	209:219	arg1	inflammation					221:232	alleviating inflammation	209:232	alleviating inflammation	209:232	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	8	77	from	concentration	1674:1686	arg1	samples					1706:1712	fecal samples	1700:1712	fecal samples	1700:1712	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	3	78	theme	Methods	571:577	arg1	Alterations					579:589	Methods Alterations	571:589	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses	571:737	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	4	79	theme	LPS	955:957	arg1	administration					959:972	LPS administration	955:972	LPS administration	955:972	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	3	80	theme	acute	779:783	arg1	injury					796:801	LPS-induced acute intestinal injury	767:801	LPS-induced acute intestinal injury in C57BL/6J mice	767:818	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	6	81	theme	T	1375:1375	arg1	cells					1377:1381	regulatory T cells	1364:1381	regulatory T cells	1364:1381	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	6	82	from	proportion	1350:1359	arg1	node					1407:1410	the mesenteric lymph node	1386:1410	the mesenteric lymph node	1386:1410	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	5	83	theme	B.	1134:1135	arg1	FTJS7K1					1146:1152	B. vulgatus FTJS7K1	1134:1152	B. vulgatus FTJS7K1	1134:1152	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	3	84	theme	intestinal	598:607	arg1	microbiota					609:618	the intestinal microbiota	594:618	the intestinal microbiota	594:618	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	7	85	dep	B.	1446:1447	arg1	vulgatus					1449:1456	vulgatus	1449:1456	vulgatus	1449:1456	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	4	86	theme	colon	930:934	arg1	tissues					936:942	the mouse colon tissues	920:942	the mouse colon tissues 24 h after LPS administration	920:972	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	6	87	theme	LPS-induced	1269:1279	arg1	changes					1281:1287	the LPS-induced changes	1265:1287	the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node	1265:1410	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	6	88	from	changes	1281:1287	arg1	proportion					1350:1359	the proportion	1346:1359	the proportion of regulatory T cells in the mesenteric lymph node	1346:1410	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	6	88	from	changes	1281:1287	arg1	tissues					1330:1336	the colon tissues	1320:1336	the colon tissues	1320:1336	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	6	88	from	changes	1281:1287	arg1	expression					1306:1315	cytokine mRNA expression	1292:1315	cytokine mRNA expression	1292:1315	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	3	89	theme	C57BL/6J	806:813	arg1	mice					815:818	C57BL/6J mice	806:818	C57BL/6J mice	806:818	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	9	90	theme	FTJS7K1	1797:1803	arg1	roles					1776:1780	these protective roles	1759:1780	these protective roles of B. vulgatus FTJS7K1	1759:1803	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	2	91	from	injury	546:551	arg1	mice					565:568	C57BL/6J mice	556:568	C57BL/6J mice	556:568	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	0	92	theme	acute	96:100	arg1	injury					113:118	lipopolysaccharide-induced acute intestinal injury	69:118	lipopolysaccharide-induced acute intestinal injury	69:118	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	3	93	theme	epithelial	632:641	arg1	permeability					643:654	intestinal epithelial permeability	621:654	intestinal epithelial permeability	621:654	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	5	94	dep	B.	1008:1009	arg1	vulgatus					1011:1018	vulgatus	1011:1018	vulgatus	1011:1018	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	10	95	theme	production	2047:2056	arg1	modulation					2024:2033	modulation	2024:2033	modulation of cytokine production in the colon tissue	2024:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	10	95	theme	production	2047:2056	arg1	regulation					2082:2091	regulation	2082:2091	regulation of the structure of the gut microbiota	2082:2130	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	0	96	theme	functional	142:151	arg1	genes					153:157	their underlying functional genes	125:157	their underlying functional genes	125:157	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	5	97	theme	architectural	1178:1190	arg1	integrity					1192:1200	the architectural integrity	1174:1200	the architectural integrity of the intestinal epithelium	1174:1229	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	7	98	theme	Faecalibaculum	1592:1605	arg1	abundance					1579:1587	abundance	1579:1587	abundance of Faecalibaculum	1579:1605	Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum.					
35127162	2	99	theme	intestinal	535:544	arg1	injury					546:551	lipopolysaccharide (LPS)-induced acute intestinal injury	496:551	lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice	496:568	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	1	100	dep	Introduction	160:171	arg1	roles					177:181	The roles	173:181	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury	160:254	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	6	101	theme	mesenteric	1390:1399	arg1	node					1407:1410	the mesenteric lymph node	1386:1410	the mesenteric lymph node	1386:1410	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	5	102	theme	FTJS7K1	1146:1152	arg1	administration					1116:1129	only the administration	1107:1129	only the administration of B. vulgatus FTJS7K1	1107:1152	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	5	102	theme	FTJS7K1	1146:1152	arg1	able					1158:1161	able	1158:1161	able	1158:1161	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	10	103	from	modulation	2024:2033	arg1	tissue					2071:2076	the colon tissue	2061:2076	the colon tissue	2061:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	10	104	theme	structure	2100:2108	arg1	modulation					2024:2033	modulation	2024:2033	modulation of cytokine production in the colon tissue	2024:2076	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	10	104	theme	structure	2100:2108	arg1	regulation					2082:2091	regulation	2082:2091	regulation of the structure of the gut microbiota	2082:2130	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	2	105	theme	-induced	520:527	arg1	injury					546:551	lipopolysaccharide (LPS)-induced acute intestinal injury	496:551	lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice	496:568	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	0	106	theme	underlying	131:140	arg1	genes					153:157	their underlying functional genes	125:157	their underlying functional genes	125:157	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	10	107	theme	FTJS7K1	1999:2005	arg1	role					1979:1982	the protective role	1964:1982	the protective role of B. vulgatus FTJS7K1	1964:2005	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	1	108	from	Introduction	160:171	arg1	inflammation					221:232	alleviating inflammation	209:232	alleviating inflammation	209:232	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	1	108	from	Introduction	160:171	arg1	injury					249:254	intestinal injury	238:254	intestinal injury	238:254	Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus.					
35127162	9	109	theme	protective	1765:1774	arg1	roles					1776:1780	these protective roles	1759:1780	these protective roles of B. vulgatus FTJS7K1	1759:1803	The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs).					
35127162	3	110	from	injury	796:801	arg1	mice					815:818	C57BL/6J mice	806:818	C57BL/6J mice	806:818	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	0	111	theme	different	22:30	arg1	strains					53:59	different Bacteroides vulgatus strains	22:59	different Bacteroides vulgatus strains	22:59	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	5	112	theme	different	998:1006	arg1	strains					1020:1026	different B. vulgatus strains	998:1026	different B. vulgatus strains	998:1026	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	11	113	theme	genus	2200:2204	arg1	screening					2171:2179	the screening	2167:2179	the screening of the Bacteroides genus for next-generation probiotics	2167:2235	These results provide support for the screening of the Bacteroides genus for next-generation probiotics.					
35127162	8	114	theme	B.	1612:1613	arg1	group					1632:1636	The B. vulgatus FTJS7K1 group	1608:1636	The B. vulgatus FTJS7K1 group	1608:1636	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	3	115	theme	short-chain	673:683	arg1	SCFA					697:700	SCFA	697:700	SCFA	697:700	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	3	115	theme	short-chain	673:683	arg1	acid					691:694	short-chain fatty acid	673:694	short-chain fatty acid (SCFA) concentration	673:715	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	0	116	theme	vulgatus	44:51	arg1	strains					53:59	different Bacteroides vulgatus strains	22:59	different Bacteroides vulgatus strains	22:59	Protective effects of different Bacteroides vulgatus strains against lipopolysaccharide-induced acute intestinal injury, and their underlying functional genes.					
35127162	8	117	theme	FTJS7K1	1624:1630	arg1	group					1632:1636	The B. vulgatus FTJS7K1 group	1608:1636	The B. vulgatus FTJS7K1 group	1608:1636	The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples.					
35127162	4	118	theme	significant	862:872	arg1	change					874:879	a significant change	860:879	a significant change in cytokine expression	860:902	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	5	119	theme	intestinal	1209:1218	arg1	epithelium					1220:1229	the intestinal epithelium	1205:1229	the intestinal epithelium	1205:1229	Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium.					
35127162	10	120	dep	Conclusions	1926:1936	arg1	suggest					1951:1957	suggest	1951:1957	suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota	1951:2130	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	3	121	theme	acid	691:694	arg1	concentration					703:715	short-chain fatty acid (SCFA) concentration	673:715	short-chain fatty acid (SCFA) concentration	673:715	Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice.					
35127162	6	122	dep	B.	1232:1233	arg1	vulgatus					1235:1242	vulgatus	1235:1242	vulgatus	1235:1242	B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node.					
35127162	10	123	theme	protective	1968:1977	arg1	role					1979:1982	the protective role	1964:1982	the protective role of B. vulgatus FTJS7K1	1964:2005	Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota.					
35127162	4	124	theme	histological	836:847	arg1	damage					849:854	Severe histological damage	829:854	Severe histological damage	829:854	Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration.					
35127162	2	125	theme	B.	384:385	arg1	strains					396:402	four B. vulgatus strains	379:402	four B. vulgatus strains	379:402	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
35127162	2	125	theme	B.	384:385	arg1	Objectives					353:362	Objectives	353:362	Objectives In this study	353:376	Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice.					
32384107	2	0	theme	gluten	553:558	arg1	parameters					601:610	Farinograph parameters	589:610	Farinograph parameters	589:610	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	0	theme	gluten	553:558	arg1	index					560:564	gluten index	553:564	gluten index	553:564	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	0	theme	gluten	553:558	arg1	sedimentation					574:586	Zeleny sedimentation	567:586	Zeleny sedimentation	567:586	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	4	1	theme	E	943:943	arg1	interactions					945:956	G × E interactions	939:956	G × E interactions	939:956	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	9	2	theme	total	1946:1950	arg1	variance					1952:1959	the total variance	1942:1959	the total variance	1942:1959	Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance.					
32384107	7	3	theme	WE-pentosan	1623:1633	arg1	contents					1611:1618	more stable contents	1599:1618	more stable contents of WE-pentosan	1599:1633	The genotypes exhibiting higher stability of content of TOT-pentosan also showed more stable contents of WE-pentosan.					
32384107	8	4	theme	contents	1694:1701	arg1	stability					1681:1689	the stability	1677:1689	the stability of contents of WE-pentosan and WE-AX	1677:1726	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	8	5	theme	WE-pentosan	1706:1716	arg1	contents					1694:1701	contents	1694:1701	contents of WE-pentosan and WE-AX	1694:1726	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	0	6	theme	arabinoxylan	58:69	arg1	level					49:53	increased level	39:53	increased level of arabinoxylan	39:69	Stability analysis of wheat lines with increased level of arabinoxylan.					
32384107	8	7	theme	WE-AX	1722:1726	arg1	contents					1694:1701	contents	1694:1701	contents of WE-pentosan and WE-AX	1694:1726	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	2	8	theme	wheat	306:310	arg1	Ukrainka					340:347	Ukrainka	340:347	Ukrainka	340:347	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	8	theme	wheat	306:310	arg1	varieties					312:320	three Central European wheat varieties	283:320	three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka)	283:348	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	9	theme	Central	289:295	arg1	Ukrainka					340:347	Ukrainka	340:347	Ukrainka	340:347	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	9	theme	Central	289:295	arg1	varieties					312:320	three Central European wheat varieties	283:320	three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka)	283:348	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	1	10	theme	different	190:198	arg1	environments					200:211	different environments	190:211	different environments	190:211	Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments.					
32384107	2	11	theme	gluten	496:501	arg1	content					503:509	gluten content	496:509	gluten content	496:509	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	11	theme	gluten	496:501	arg1	content					487:493	protein content	479:493	protein content	479:493	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	9	12	with	0.829	1896:1900	arg1	genotype					1911:1918	the genotype	1907:1918	the genotype determining 38.67% of the total variance	1907:1959	Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance.					
32384107	3	13	theme	traits	722:727	arg1	heritability					696:707	heritability	696:707	heritability	696:707	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	3	13	theme	traits	722:727	arg1	stability					682:690	stability	682:690	stability	682:690	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	2	14	theme	hectolitre	408:417	arg1	weight					419:424	hectolitre weight	408:424	hectolitre weight	408:424	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	14	theme	hectolitre	408:417	arg1	weight					442:447	thousand grain weight	427:447	thousand grain weight	427:447	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	14	theme	hectolitre	408:417	arg1	yield					456:460	flour yield	450:460	flour yield	450:460	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	7	15	theme	content	1563:1569	arg1	stability					1550:1558	higher stability	1543:1558	higher stability of content of TOT-pentosan	1543:1585	The genotypes exhibiting higher stability of content of TOT-pentosan also showed more stable contents of WE-pentosan.					
32384107	3	16	theme	relative	744:751	arg1	effects					753:759	the relative effects	740:759	the relative effects of the genotype (G) and environment (E)	740:799	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	8	17	theme	content	1762:1768	arg1	stability					1739:1747	the stability	1735:1747	the stability of the WE-AX content and AX composition	1735:1787	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	2	18	theme	variety	249:255	arg1	Yumai-34					257:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	6	19	theme	water-soluble	1389:1401	arg1	arabinoxylan					1403:1414	water-soluble arabinoxylan	1389:1414	water-soluble arabinoxylan	1389:1414	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	4	20	theme	pentosans	889:897	arg1	contents					853:860	The contents	849:860	The contents of total and water-soluble pentosans	849:897	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	8	21	theme	AX	1774:1775	arg1	composition					1777:1787	AX composition	1774:1787	AX composition	1774:1787	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	1	22	theme	outstanding	134:144	arg1	performance					146:156	outstanding performance	134:156	outstanding performance	134:156	Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments.					
32384107	9	23	theme	Water	1820:1824	arg1	absorption					1826:1835	Water absorption	1820:1835	Water absorption	1820:1835	Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance.					
32384107	2	24	from	experiment	629:638	arg1	generations					665:675	the F7-F9 generations	655:675	the F7-F9 generations	655:675	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	24	from	experiment	629:638	arg1	physical					398:405	physical	398:405	physical	398:405	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	3	25	theme	genotype	768:775	arg1	effects					753:759	the relative effects	740:759	the relative effects of the genotype (G) and environment (E)	740:799	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	2	26	dep	Ukrainka	340:347	arg1	Mv-Mambo					330:337	Mv-Mambo	330:337	Mv-Mambo	330:337	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	5	27	theme	pentosans	1116:1124	arg1	component					1099:1107	The main component	1090:1107	The main component of the pentosans	1090:1124	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	27	theme	pentosans	1116:1124	arg1	amount					1131:1136	the amount	1127:1136	the amount	1127:1136	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	27	theme	pentosans	1116:1124	arg1	composition					1142:1152	composition	1142:1152	composition	1142:1152	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	27	theme	pentosans	1116:1124	arg1	arabinoxylan					1186:1197	the arabinoxylan	1182:1197	the arabinoxylan (AX)	1182:1202	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	1	28	theme	Plant	72:76	arg1	breeders					78:85	Plant breeders	72:85	Plant breeders	72:85	Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments.					
32384107	2	29	theme	three-year	618:627	arg1	2013-2015					641:649	2013-2015	641:649	2013-2015	641:649	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	29	theme	three-year	618:627	arg1	experiment					629:638	a three-year experiment	616:638	a three-year experiment (2013-2015) in the F7-F9 generations	616:675	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	30	theme	selected	357:364	arg1	lines					374:378	31 selected high-AX lines	354:378	31 selected high-AX lines	354:378	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	0	31	theme	Stability	0:8	arg1	analysis					10:17	Stability analysis	0:17	Stability analysis of wheat lines with increased level of arabinoxylan.	0:70	Stability analysis of wheat lines with increased level of arabinoxylan.					
32384107	4	32	theme	×	941:941	arg1	interactions					945:956	G × E interactions	939:956	G × E interactions	939:956	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	1	33	from	quality	179:185	arg1	environments					200:211	different environments	190:211	different environments	190:211	Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments.					
32384107	2	34	dep	compositional	464:476	arg1	content					503:509	gluten content	496:509	gluten content	496:509	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	34	dep	compositional	464:476	arg1	pentosan					512:519	pentosan	512:519	pentosan	512:519	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	34	dep	compositional	464:476	arg1	content					487:493	protein content	479:493	protein content	479:493	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	3	35	theme	grain	830:834	arg1	composition					836:846	grain composition	830:846	grain composition	830:846	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	9	36	theme	0.829	1896:1900	arg1	heritability					1880:1891	a heritability	1878:1891	a heritability of 0.829 with the genotype determining 38.67% of the total variance	1878:1959	Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance.					
32384107	0	37	theme	lines	28:32	arg1	analysis					10:17	Stability analysis	0:17	Stability analysis of wheat lines with increased level of arabinoxylan.	0:70	Stability analysis of wheat lines with increased level of arabinoxylan.					
32384107	2	38	theme	grain	436:440	arg1	weight					419:424	hectolitre weight	408:424	hectolitre weight	408:424	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	38	theme	grain	436:440	arg1	weight					442:447	thousand grain weight	427:447	thousand grain weight	427:447	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	weight					419:424	hectolitre weight	408:424	hectolitre weight	408:424	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	yield					456:460	flour yield	450:460	flour yield	450:460	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	index					560:564	gluten index	553:564	gluten index	553:564	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	parameters					601:610	Farinograph parameters	589:610	Farinograph parameters	589:610	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	sedimentation					574:586	Zeleny sedimentation	567:586	Zeleny sedimentation	567:586	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	weight					442:447	thousand grain weight	427:447	thousand grain weight	427:447	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	39	dep	physical	398:405	arg1	traits					545:550	quality traits	537:550	quality traits	537:550	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	0	40	theme	increased	39:47	arg1	level					49:53	increased level	39:53	increased level of arabinoxylan	39:69	Stability analysis of wheat lines with increased level of arabinoxylan.					
32384107	4	41	theme	total	983:987	arg1	-pentosan					994:1002	total (TOT)-pentosan	983:1002	total (TOT)-pentosan	983:1002	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	4	41	theme	total	983:987	arg1	TOT					990:992	TOT	990:992	TOT	990:992	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	5	42	theme	arabinoxylan	1186:1197	arg1	ratio					1172:1176	arabinose:xylose ratio	1155:1176	arabinose:xylose ratio	1155:1176	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	42	theme	arabinoxylan	1186:1197	arg1	composition					1142:1152	composition	1142:1152	composition	1142:1152	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	42	theme	arabinoxylan	1186:1197	arg1	component					1099:1107	The main component	1090:1107	The main component of the pentosans	1090:1124	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	42	theme	arabinoxylan	1186:1197	arg1	amount					1131:1136	the amount	1127:1136	the amount	1127:1136	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	42	theme	arabinoxylan	1186:1197	arg1	arabinoxylan					1186:1197	the arabinoxylan	1182:1197	the arabinoxylan (AX)	1182:1202	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	2	43	from	physical	398:405	arg1	2013-2015					641:649	2013-2015	641:649	2013-2015	641:649	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	43	from	physical	398:405	arg1	experiment					629:638	a three-year experiment	616:638	a three-year experiment (2013-2015) in the F7-F9 generations	616:675	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	9	44	theme	variance	1952:1959	arg1	variance					1952:1959	the total variance	1942:1959	the total variance	1942:1959	Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance.					
32384107	9	44	theme	variance	1952:1959	arg1	%					1937:1937	38.67%	1932:1937	38.67% of the total variance	1932:1959	Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance.					
32384107	2	45	theme	quality	537:543	arg1	traits					545:550	quality traits	537:550	quality traits	537:550	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	7	46	theme	stable	1604:1609	arg1	contents					1611:1618	more stable contents	1599:1618	more stable contents of WE-pentosan	1599:1633	The genotypes exhibiting higher stability of content of TOT-pentosan also showed more stable contents of WE-pentosan.					
32384107	1	47	theme	high	163:166	arg1	quality					179:185	high and stable quality	163:185	high and stable quality in different environments	163:211	Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments.					
32384107	2	48	theme	Zeleny	567:572	arg1	index					560:564	gluten index	553:564	gluten index	553:564	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	48	theme	Zeleny	567:572	arg1	sedimentation					574:586	Zeleny sedimentation	567:586	Zeleny sedimentation	567:586	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	1	49	theme	stable	172:177	arg1	quality					179:185	high and stable quality	163:185	high and stable quality in different environments	163:211	Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments.					
32384107	6	50	dep	significant	1487:1497	arg1	0.721					1510:1514	0.721	1510:1514	0.721	1510:1514	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	50	dep	significant	1487:1497	arg1	heritability					1449:1460	the heritability	1445:1460	the heritability of these traits	1445:1476	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	50	dep	significant	1487:1497	arg1	0.840					1500:1504	0.840	1500:1504	0.840	1500:1504	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	50	dep	significant	1487:1497	arg1	significant					1487:1497	significant	1487:1497	significant	1487:1497	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	7	51	theme	TOT-pentosan	1574:1585	arg1	content					1563:1569	content	1563:1569	content of TOT-pentosan	1563:1585	The genotypes exhibiting higher stability of content of TOT-pentosan also showed more stable contents of WE-pentosan.					
32384107	2	52	theme	European	297:304	arg1	Ukrainka					340:347	Ukrainka	340:347	Ukrainka	340:347	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	52	theme	European	297:304	arg1	varieties					312:320	three Central European wheat varieties	283:320	three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka)	283:348	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	4	53	theme	-pentosan	994:1002	arg1	heritability					967:978	the heritability	963:978	the heritability of total (TOT)-pentosan	963:1002	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	4	53	theme	-pentosan	994:1002	arg1	lower					1022:1026	lower	1022:1026	lower	1022:1026	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	2	54	theme	AX	237:238	arg1	Yumai-34					257:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	4	55	theme	water-extractable	1049:1065	arg1	0.825					1082:1086	0.825	1082:1086	0.825	1082:1086	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	4	55	theme	water-extractable	1049:1065	arg1	-pentosan					1071:1079	water-extractable (WE)-pentosan	1049:1079	water-extractable (WE)-pentosan (0.825)	1049:1087	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	8	56	theme	WE-AX	1756:1760	arg1	content					1762:1768	the WE-AX content	1752:1768	the WE-AX content	1752:1768	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	2	57	theme	high-arabinoxylan	218:234	arg1	Yumai-34					257:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	3	58	theme	different	712:720	arg1	effects					753:759	the relative effects	740:759	the relative effects of the genotype (G) and environment (E)	740:799	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	3	58	theme	different	712:720	arg1	traits					722:727	different traits	712:727	different traits	712:727	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	6	59	theme	arabinoxylan	1403:1414	arg1	amount					1379:1384	the amount	1375:1384	the amount of water-soluble arabinoxylan	1375:1414	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	59	theme	arabinoxylan	1403:1414	arg1	arabinoxylan					1403:1414	water-soluble arabinoxylan	1389:1414	water-soluble arabinoxylan	1389:1414	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	3	60	theme	environment	785:795	arg1	effects					753:759	the relative effects	740:759	the relative effects of the genotype (G) and environment (E)	740:799	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	8	61	theme	composition	1777:1787	arg1	stability					1739:1747	the stability	1735:1747	the stability of the WE-AX content and AX composition	1735:1787	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	2	62	theme	Chinese	241:247	arg1	Yumai-34					257:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34	214:264	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	4	63	dep	lower	1022:1026	arg1	0.341					1029:1033	0.341	1029:1033	0.341	1029:1033	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	4	64	theme	water-soluble	875:887	arg1	pentosans					889:897	total and water-soluble pentosans	865:897	total and water-soluble pentosans	865:897	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	2	65	theme	F7-F9	659:663	arg1	generations					665:675	the F7-F9 generations	655:675	the F7-F9 generations	655:675	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	3	66	dep	stability	682:690	arg1	The					678:680	The	678:680	The	678:680	The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition.					
32384107	7	67	theme	higher	1543:1548	arg1	stability					1550:1558	higher stability	1543:1558	higher stability of content of TOT-pentosan	1543:1585	The genotypes exhibiting higher stability of content of TOT-pentosan also showed more stable contents of WE-pentosan.					
32384107	2	68	theme	flour	450:454	arg1	weight					419:424	hectolitre weight	408:424	hectolitre weight	408:424	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	68	theme	flour	450:454	arg1	yield					456:460	flour yield	450:460	flour yield	450:460	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	5	69	theme	parameters	1301:1310	arg1	heritability					1279:1290	the broader heritability	1267:1290	the broader heritability of these parameters	1267:1310	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	69	theme	parameters	1301:1310	arg1	0.516					1317:1321	0.516	1317:1321	0.516	1317:1321	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	6	70	theme	traits	1471:1476	arg1	0.721					1510:1514	0.721	1510:1514	0.721	1510:1514	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	70	theme	traits	1471:1476	arg1	heritability					1449:1460	the heritability	1445:1460	the heritability of these traits	1445:1476	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	70	theme	traits	1471:1476	arg1	significant					1487:1497	significant	1487:1497	significant	1487:1497	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	70	theme	traits	1471:1476	arg1	0.840					1500:1504	0.840	1500:1504	0.840	1500:1504	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	6	70	theme	traits	1471:1476	arg1	composition					1424:1434	composition	1424:1434	composition	1424:1434	However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721).					
32384107	2	71	theme	protein	479:485	arg1	content					503:509	gluten content	496:509	gluten content	496:509	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	71	theme	protein	479:485	arg1	pentosan					512:519	pentosan	512:519	pentosan	512:519	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	71	theme	protein	479:485	arg1	content					487:493	protein content	479:493	protein content	479:493	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	72	theme	Farinograph	589:599	arg1	parameters					601:610	Farinograph parameters	589:610	Farinograph parameters	589:610	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	72	theme	Farinograph	589:599	arg1	index					560:564	gluten index	553:564	gluten index	553:564	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	10	73	theme	Many	1962:1965	arg1	lines					1967:1971	Many lines	1962:1971	Many lines	1962:1971	Many lines were grouped in the GGE biplot, indicating that they did not significantly differ stability.					
32384107	5	74	theme	xylose	1165:1170	arg1	ratio					1172:1176	arabinose:xylose ratio	1155:1176	arabinose:xylose ratio	1155:1176	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	74	theme	xylose	1165:1170	arg1	amount					1131:1136	the amount	1127:1136	the amount	1127:1136	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	74	theme	xylose	1165:1170	arg1	arabinoxylan					1186:1197	the arabinoxylan	1182:1197	the arabinoxylan (AX)	1182:1202	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	4	75	theme	total	865:869	arg1	pentosans					889:897	total and water-soluble pentosans	865:897	total and water-soluble pentosans	865:897	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	5	76	theme	broader	1271:1277	arg1	heritability					1279:1290	the broader heritability	1267:1290	the broader heritability of these parameters	1267:1310	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	76	theme	broader	1271:1277	arg1	0.516					1317:1321	0.516	1317:1321	0.516	1317:1321	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	2	77	theme	high-AX	366:372	arg1	lines					374:378	31 selected high-AX lines	354:378	31 selected high-AX lines	354:378	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	8	78	theme	positive	1648:1655	arg1	correlation					1657:1667	a positive correlation	1646:1667	a positive correlation between the stability of contents of WE-pentosan and WE-AX	1646:1726	There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated.					
32384107	0	79	theme	wheat	22:26	arg1	lines					28:32	wheat lines	22:32	wheat lines	22:32	Stability analysis of wheat lines with increased level of arabinoxylan.					
32384107	4	80	theme	G	939:939	arg1	interactions					945:956	G × E interactions	939:956	G × E interactions	939:956	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	5	81	theme	arabinose	1155:1163	arg1	ratio					1172:1176	arabinose:xylose ratio	1155:1176	arabinose:xylose ratio	1155:1176	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	81	theme	arabinose	1155:1163	arg1	amount					1131:1136	the amount	1127:1136	the amount	1127:1136	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	81	theme	arabinose	1155:1163	arg1	arabinoxylan					1186:1197	the arabinoxylan	1182:1197	the arabinoxylan (AX)	1182:1202	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	82	dep	arabinoxylan	1186:1197	arg1	AX					1200:1201	AX	1200:1201	AX	1200:1201	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	2	83	theme	thousand	427:434	arg1	weight					419:424	hectolitre weight	408:424	hectolitre weight	408:424	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	2	83	theme	thousand	427:434	arg1	weight					442:447	thousand grain weight	427:447	thousand grain weight	427:447	The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013-2015) in the F7-F9 generations.					
32384107	0	84	with	analysis	10:17	arg1	level					49:53	increased level	39:53	increased level of arabinoxylan	39:69	Stability analysis of wheat lines with increased level of arabinoxylan.					
32384107	10	85	theme	GGE	1993:1995	arg1	biplot					1997:2002	the GGE biplot	1989:2002	the GGE biplot	1989:2002	Many lines were grouped in the GGE biplot, indicating that they did not significantly differ stability.					
32384107	4	86	dep	-pentosan	1071:1079	arg1	WE					1068:1069	WE	1068:1069	WE	1068:1069	The contents of total and water-soluble pentosans were significantly affected by G, E and G × E interactions, but the heritability of total (TOT)-pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825).					
32384107	5	87	theme	main	1094:1097	arg1	component					1099:1107	The main component	1090:1107	The main component of the pentosans	1090:1124	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	87	theme	main	1094:1097	arg1	amount					1131:1136	the amount	1127:1136	the amount	1127:1136	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	87	theme	main	1094:1097	arg1	composition					1142:1152	composition	1142:1152	composition	1142:1152	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
32384107	5	87	theme	main	1094:1097	arg1	arabinoxylan					1186:1197	the arabinoxylan	1182:1197	the arabinoxylan (AX)	1182:1202	The main component of the pentosans, the amount and composition (arabinose:xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772.					
33203758	11	0	theme	human	1864:1868	arg1	microbiome					1874:1883	the human gut microbiome	1860:1883	the human gut microbiome	1860:1883	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	5	1	theme	microbiome	607:616	arg1	features					618:625	microbiome features	607:625	microbiome features of asymptomatic and symptomatic individuals	607:669	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	1	2	theme	heavy	132:136	arg1	toll					138:141	a heavy toll	130:141	a heavy toll on worldwide public health	130:168	Norovirus infections take a heavy toll on worldwide public health.					
33203758	11	3	theme	gut	1729:1731	arg1	role					1744:1747	the gut microbiome role	1725:1747	the gut microbiome role	1725:1747	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	13	4	theme	infections	2259:2268	arg1	resistance					2218:2227	resistance	2218:2227	resistance to	2218:2230	The results have implications for understanding resistance to and treatment of norovirus infections.					
33203758	13	4	theme	infections	2259:2268	arg1	treatment					2236:2244	treatment	2236:2244	treatment of norovirus infections	2236:2268	The results have implications for understanding resistance to and treatment of norovirus infections.					
33203758	7	5	theme	glycans	1077:1083	arg1	metabolism					1063:1072	the metabolism	1059:1072	the metabolism of glycans and sphingolipids	1059:1101	These compositional differences were accompanied by differences in genes involved in the metabolism of glycans and sphingolipids that may aid in host resilience to infection.					
33203758	12	6	from	responses	2132:2140	arg1	study					2163:2167	a human challenge study	2145:2167	a human challenge study	2145:2167	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	10	7	theme	subjects	1473:1480	arg1	microbiomes					1446:1456	the microbiomes	1442:1456	the microbiomes of symptomatic subjects	1442:1480	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	13	8	theme	norovirus	2249:2257	arg1	infections					2259:2268	norovirus infections	2249:2268	norovirus infections	2249:2268	The results have implications for understanding resistance to and treatment of norovirus infections.					
33203758	7	9	theme	host	1119:1122	arg1	resilience					1124:1133	host resilience	1119:1133	host resilience to infection	1119:1146	These compositional differences were accompanied by differences in genes involved in the metabolism of glycans and sphingolipids that may aid in host resilience to infection.					
33203758	5	10	from	time	747:750	arg1	individuals					767:777	symptomatic individuals	755:777	symptomatic individuals	755:777	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	10	11	theme	symptomatic	1461:1471	arg1	subjects					1473:1480	symptomatic subjects	1461:1480	symptomatic subjects	1461:1480	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	1	12	from	toll	138:141	arg1	health					163:168	worldwide public health	146:168	worldwide public health	146:168	Norovirus infections take a heavy toll on worldwide public health.					
33203758	11	13	theme	health	1821:1826	arg1	outcomes.IMPORTANCE					1828:1846	long-term health outcomes.IMPORTANCE	1811:1846	long-term health outcomes.IMPORTANCE	1811:1846	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	12	14	theme	symptomatic	2070:2080	arg1	microbiomes					2082:2092	symptomatic microbiomes	2070:2092	symptomatic microbiomes	2070:2092	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	11	15	theme	host	1752:1755	arg1	susceptibility					1757:1770	host susceptibility	1752:1770	host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood	1752:1985	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	3	16	contain	has	437:439	arg2	implications					451:462	important implications	441:462	important implications for diagnostics and host recovery	441:496	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	3	16	contain	has	437:439	arg1	infection					420:428	norovirus infection	410:428	norovirus infection	410:428	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	5	17	theme	asymptomatic	630:641	arg1	individuals					659:669	asymptomatic and symptomatic individuals	630:669	asymptomatic and symptomatic individuals	630:669	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	11	18	theme	important	1689:1697	arg1	context					1699:1705	important context	1689:1705	important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood	1689:1985	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	4	19	theme	subjects	545:552	arg1	microbiomes					530:540	the gut microbiomes	522:540	the gut microbiomes of subjects enrolled in a norovirus challenge study	522:592	Here, we characterized the gut microbiomes of subjects enrolled in a norovirus challenge study.					
33203758	0	20	theme	Challenge	87:95	arg1	Study					97:101	a Human Challenge Study	79:101	a Human Challenge Study	79:101	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.					
33203758	11	21	theme	microbiome	1733:1742	arg1	role					1744:1747	the gut microbiome role	1725:1747	the gut microbiome role	1725:1747	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	12	22	theme	important	2008:2016	arg1	data					2018:2021	important data	2008:2021	important data	2008:2021	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	10	23	attach	linked	1528:1533	arg2	glgC					1517:1520	the adenylyltransferase glgC	1493:1520	the adenylyltransferase glgC	1493:1520	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	10	23	attach	linked	1528:1533	arg1	metabolism					1545:1554	glycan metabolism	1538:1554	glycan metabolism	1538:1554	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	10	23	attach	linked	1528:1533	arg1	signaling					1570:1578	cell-cell signaling	1560:1578	cell-cell signaling	1560:1578	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	10	23	attach	linked	1528:1533	arg2	Genes					1424:1428	Genes	1424:1428	Genes	1424:1428	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	3	24	theme	microbiome	387:396	arg1	response					398:405	the microbiome response	383:405	the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery	383:496	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	0	25	theme	Human	81:85	arg1	Study					97:101	a Human Challenge Study	79:101	a Human Challenge Study	79:101	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.					
33203758	11	26	theme	long-term	1811:1819	arg1	outcomes.IMPORTANCE					1828:1846	long-term health outcomes.IMPORTANCE	1811:1846	long-term health outcomes.IMPORTANCE	1811:1846	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	3	27	dep	nature	360:365	arg1	the					356:358	the	356:358	the	356:358	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	3	28	theme	response	398:405	arg1	nature					360:365	nature	360:365	nature	360:365	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	3	28	theme	response	398:405	arg1	duration					371:378	duration	371:378	duration	371:378	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	10	29	theme	infection	1648:1656	arg1	outcome					1658:1664	infection outcome	1648:1664	infection outcome	1648:1664	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	11	30	theme	gut	1870:1872	arg1	microbiome					1874:1883	the human gut microbiome	1860:1883	the human gut microbiome	1860:1883	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	12	31	theme	challenge	2153:2161	arg1	study					2163:2167	a human challenge study	2145:2167	a human challenge study	2145:2167	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	11	32	theme	microbiome	1874:1883	arg1	role					1852:1855	The role	1848:1855	The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic	1848:1964	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	12	33	attach	links	2098:2102	arg1	responses					2132:2140	infection responses	2122:2140	infection responses in a human challenge study	2122:2167	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	12	33	attach	links	2098:2102	arg2	study					1993:1997	This study	1988:1997	This study	1988:1997	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	1	34	theme	public	156:161	arg1	health					163:168	worldwide public health	146:168	worldwide public health	146:168	Norovirus infections take a heavy toll on worldwide public health.					
33203758	6	35	theme	subjects	964:971	arg1	microbiomes					937:947	the microbiomes	933:947	the microbiomes of symptomatic subjects	933:971	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	7	36	theme	sphingolipids	1089:1101	arg1	metabolism					1063:1072	the metabolism	1059:1072	the metabolism of glycans and sphingolipids	1059:1101	These compositional differences were accompanied by differences in genes involved in the metabolism of glycans and sphingolipids that may aid in host resilience to infection.					
33203758	2	37	theme	microbiome	270:279	arg1	role					254:257	the role	250:257	the role of the gut microbiome in determining infection outcome	250:312	While progress has been made toward understanding host responses to infection, the role of the gut microbiome in determining infection outcome is unknown.					
33203758	2	37	theme	microbiome	270:279	arg1	unknown					317:323	unknown	317:323	unknown	317:323	While progress has been made toward understanding host responses to infection, the role of the gut microbiome in determining infection outcome is unknown.					
33203758	11	38	dep	susceptibility	1757:1770	arg1	understood					1976:1985	understood	1976:1985	is poorly understood	1966:1985	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	6	39	theme	symptomatic	952:962	arg1	subjects					964:971	symptomatic subjects	952:971	symptomatic subjects	952:971	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	2	40	theme	gut	266:268	arg1	microbiome					270:279	the gut microbiome	262:279	the gut microbiome	262:279	While progress has been made toward understanding host responses to infection, the role of the gut microbiome in determining infection outcome is unknown.					
33203758	9	41	theme	same	1413:1416	arg1	time					1418:1421	the same time	1409:1421	the same time	1409:1421	In particular, Firmicutes increased immediately following the challenge, while Bacteroidetes and Proteobacteria decreased over the same time.					
33203758	4	42	theme	gut	526:528	arg1	microbiomes					530:540	the gut microbiomes	522:540	the gut microbiomes of subjects enrolled in a norovirus challenge study	522:592	Here, we characterized the gut microbiomes of subjects enrolled in a norovirus challenge study.					
33203758	5	43	theme	genome	678:683	arg1	population					686:695	the genome (population) and gene levels	674:712	population	686:695	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	6	44	theme	asymptomatic	839:850	arg1	infections					852:861	asymptomatic infections	839:861	asymptomatic infections	839:861	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	8	45	theme	human	1269:1273	arg1	hosts					1275:1279	human hosts	1269:1279	human hosts	1269:1279	We further show that microbiomes shifted in composition following infection and that recovery times were variable among human hosts.					
33203758	0	46	theme	Microbiome	20:29	arg1	Role					4:7	The Role	0:7	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.	0:102	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.					
33203758	6	47	with	subjects	825:832	arg1	infections					852:861	asymptomatic infections	839:861	asymptomatic infections	839:861	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	0	48	theme	Gut	16:18	arg1	Microbiome					20:29	the Gut Microbiome	12:29	the Gut Microbiome	12:29	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.					
33203758	13	49	contain	have	2182:2185	arg2	implications					2187:2198	implications	2187:2198	implications for understanding resistance to and treatment of norovirus infections	2187:2268	The results have implications for understanding resistance to and treatment of norovirus infections.					
33203758	13	49	contain	have	2182:2185	arg1	results					2174:2180	The results	2170:2180	The results	2170:2180	The results have implications for understanding resistance to and treatment of norovirus infections.					
33203758	6	50	theme	subjects	825:832	arg1	microbiomes					810:820	the preinfection microbiomes	793:820	the preinfection microbiomes of subjects with asymptomatic infections	793:861	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	3	51	theme	norovirus	410:418	arg1	infection					420:428	norovirus infection	410:428	norovirus infection	410:428	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	8	52	theme	recovery	1234:1241	arg1	times					1243:1247	recovery times	1234:1247	recovery times	1234:1247	We further show that microbiomes shifted in composition following infection and that recovery times were variable among human hosts.					
33203758	10	53	theme	cell-cell	1560:1568	arg1	signaling					1570:1578	cell-cell signaling	1560:1578	cell-cell signaling	1560:1578	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	12	54	theme	infection	2122:2130	arg1	responses					2132:2140	infection responses	2122:2140	infection responses in a human challenge study	2122:2167	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	3	55	theme	important	441:449	arg1	implications					451:462	important implications	441:462	important implications for diagnostics and host recovery	441:496	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	5	56	theme	symptomatic	755:765	arg1	individuals					767:777	symptomatic individuals	755:777	symptomatic individuals	755:777	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	6	57	theme	relative	921:928	arg1	Clostridia					910:919	Clostridia	910:919	Clostridia relative to the microbiomes of symptomatic subjects	910:971	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	5	58	theme	symptomatic	647:657	arg1	individuals					659:669	asymptomatic and symptomatic individuals	630:669	asymptomatic and symptomatic individuals	630:669	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	11	59	theme	norovirus	1787:1795	arg1	infection					1797:1805	symptomatic norovirus infection	1775:1805	symptomatic norovirus infection	1775:1805	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	10	60	theme	adenylyltransferase	1497:1515	arg1	glgC					1517:1520	the adenylyltransferase glgC	1493:1520	the adenylyltransferase glgC	1493:1520	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	10	61	theme	unknown	1599:1605	arg1	roles					1607:1611	as-yet unknown roles	1592:1611	as-yet unknown roles for these processes in determining infection outcome	1592:1664	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	5	62	theme	individuals	659:669	arg1	features					618:625	microbiome features	607:625	microbiome features of asymptomatic and symptomatic individuals	607:669	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	6	63	theme	preinfection	797:808	arg1	microbiomes					810:820	the preinfection microbiomes	793:820	the preinfection microbiomes of subjects with asymptomatic infections	793:861	We show that the preinfection microbiomes of subjects with asymptomatic infections were enriched in Bacteroidetes and depleted in Clostridia relative to the microbiomes of symptomatic subjects.					
33203758	11	64	theme	symptomatic	1775:1785	arg1	infection					1797:1805	symptomatic norovirus infection	1775:1805	symptomatic norovirus infection	1775:1805	These results provide important context for understanding the gut microbiome role in host susceptibility to symptomatic norovirus infection and long-term health outcomes.IMPORTANCE The role of the human gut microbiome in determining whether an individual infected with norovirus will be symptomatic is poorly understood.					
33203758	1	65	theme	Norovirus	104:112	arg1	infections					114:123	Norovirus infections	104:123	Norovirus infections	104:123	Norovirus infections take a heavy toll on worldwide public health.					
33203758	0	66	dep	Infection	54:62	arg1	Revealed					67:74	Revealed	67:74	Revealed by a Human Challenge Study	67:101	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.					
33203758	5	67	theme	gene	702:705	arg1	levels					707:712	the genome (population) and gene levels	674:712	levels	707:712	We analyzed microbiome features of asymptomatic and symptomatic individuals at the genome (population) and gene levels and assessed their response over time in symptomatic individuals.					
33203758	10	68	theme	glycan	1538:1543	arg1	metabolism					1545:1554	glycan metabolism	1538:1554	glycan metabolism	1538:1554	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	7	69	theme	compositional	980:992	arg1	differences					994:1004	These compositional differences	974:1004	These compositional differences	974:1004	These compositional differences were accompanied by differences in genes involved in the metabolism of glycans and sphingolipids that may aid in host resilience to infection.					
33203758	12	70	theme	human	2147:2151	arg1	study					2163:2167	a human challenge study	2145:2167	a human challenge study	2145:2167	This study provides important data on microbes that distinguish asymptomatic from symptomatic microbiomes and links these features to infection responses in a human challenge study.					
33203758	2	71	theme	infection	296:304	arg1	outcome					306:312	infection outcome	296:312	infection outcome	296:312	While progress has been made toward understanding host responses to infection, the role of the gut microbiome in determining infection outcome is unknown.					
33203758	10	72	theme	as-yet	1592:1597	arg1	roles					1607:1611	as-yet unknown roles	1592:1611	as-yet unknown roles for these processes in determining infection outcome	1592:1664	Genes enriched in the microbiomes of symptomatic subjects, including the adenylyltransferase glgC, were linked to glycan metabolism and cell-cell signaling, suggesting as-yet unknown roles for these processes in determining infection outcome.					
33203758	7	73	from	differences	1026:1036	arg1	genes					1041:1045	genes	1041:1045	genes involved in the metabolism of glycans and sphingolipids that may aid in host resilience to infection	1041:1146	These compositional differences were accompanied by differences in genes involved in the metabolism of glycans and sphingolipids that may aid in host resilience to infection.					
33203758	8	74	dep	microbiomes	1170:1180	arg1	shifted					1182:1188	shifted	1182:1188	microbiomes shifted in composition following infection	1170:1223	We further show that microbiomes shifted in composition following infection and that recovery times were variable among human hosts.					
33203758	1	75	theme	worldwide	146:154	arg1	health					163:168	worldwide public health	146:168	worldwide public health	146:168	Norovirus infections take a heavy toll on worldwide public health.					
33203758	4	76	theme	challenge	578:586	arg1	study					588:592	a norovirus challenge study	566:592	a norovirus challenge study	566:592	Here, we characterized the gut microbiomes of subjects enrolled in a norovirus challenge study.					
33203758	0	77	theme	Norovirus	44:52	arg1	Infection					54:62	Norovirus Infection	44:62	Norovirus Infection as Revealed by a Human Challenge Study	44:101	The Role of the Gut Microbiome in Resisting Norovirus Infection as Revealed by a Human Challenge Study.					
33203758	2	78	theme	host	221:224	arg1	responses					226:234	host responses	221:234	host responses to infection	221:247	While progress has been made toward understanding host responses to infection, the role of the gut microbiome in determining infection outcome is unknown.					
33203758	3	79	theme	host	484:487	arg1	recovery					489:496	host recovery	484:496	host recovery	484:496	Moreover, data are lacking on the nature and duration of the microbiome response to norovirus infection, which has important implications for diagnostics and host recovery.					
33203758	4	80	theme	norovirus	568:576	arg1	study					588:592	a norovirus challenge study	566:592	a norovirus challenge study	566:592	Here, we characterized the gut microbiomes of subjects enrolled in a norovirus challenge study.					
33440209	4	0	theme	concentration	731:743	arg1	value					745:749	fungicidal concentration value	720:749	fungicidal concentration value	720:749	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	9	1	theme	hydrolytic	1263:1272	arg1	enzymes					1274:1280	the hydrolytic enzymes	1259:1280	the hydrolytic enzymes	1259:1280	CRG-AgNps also significantly inactivated the hydrolytic enzymes, thus hindering the biofilm forming ability.					
33440209	1	2	theme	strains	174:180	arg1	advent					142:147	Global antimicrobial crisis and advent	110:147	advent	142:147	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	1	2	theme	strains	174:180	arg1	crisis					131:136	Global antimicrobial crisis and advent	110:147	crisis	131:136	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	0	3	theme	Candida	93:99	arg1	species					101:107	Candida species	93:107	Candida species	93:107	Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species.					
33440209	8	4	theme	concentration-dependent	1085:1107	arg1	manner					1109:1114	a concentration-dependent manner	1083:1114	a concentration-dependent manner	1083:1114	Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content.					
33440209	7	5	theme	pore	1031:1034	arg1	formation					1036:1044	pore formation	1031:1044	pore formation	1031:1044	Microscopic studies indicate that CRG-AgNps caused morphological damage through membrane disruption and pore formation.					
33440209	3	6	theme	reactive	542:549	arg1	ROS					567:569	ROS	567:569	ROS	567:569	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	6	theme	reactive	542:549	arg1	species					558:564	reactive oxygen species	542:564	reactive oxygen species (ROS) generation	542:581	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	7	theme	microwave	392:400	arg1	AgNps					444:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	7	8	theme	membrane	1007:1014	arg1	disruption					1016:1025	membrane disruption	1007:1025	membrane disruption	1007:1025	Microscopic studies indicate that CRG-AgNps caused morphological damage through membrane disruption and pore formation.					
33440209	10	9	theme	multiple	1500:1507	arg1	mechanisms					1520:1529	multiple signalling mechanisms	1500:1529	multiple signalling mechanisms	1500:1529	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	3	10	from	generation	572:581	arg1	terms					517:521	terms	517:521	terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix	517:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	11	theme	capped	437:442	arg1	AgNps					444:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	12	theme	irradiated	402:411	arg1	AgNps					444:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	1	13	contain	has	182:184	arg2	management					219:228	substantially distressed disease management	186:228	substantially distressed disease management for clinicians	186:243	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	1	13	contain	has	182:184	arg1	advent					142:147	Global antimicrobial crisis and advent	110:147	advent	142:147	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	1	13	contain	has	182:184	arg1	crisis					131:136	Global antimicrobial crisis and advent	110:147	crisis	131:136	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	6	14	theme	biofilm	843:849	arg1	%					830:830	The 80%	824:830	The 80% of Candida biofilm	824:849	The 80% of Candida biofilm was inhibited and eradicated by CRG-AgNps at a concentration of ~300 μg/mL.					
33440209	6	14	theme	biofilm	843:849	arg1	biofilm					843:849	Candida biofilm	835:849	Candida biofilm	835:849	The 80% of Candida biofilm was inhibited and eradicated by CRG-AgNps at a concentration of ~300 μg/mL.					
33440209	2	15	theme	remedial	324:331	arg1	option					333:338	an excellent alternative remedial option	299:338	an excellent alternative remedial option	299:338	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	10	16	theme	signalling	1509:1518	arg1	mechanisms					1520:1529	multiple signalling mechanisms	1500:1529	multiple signalling mechanisms	1500:1529	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	6	17	theme	Candida	835:841	arg1	biofilm					843:849	Candida biofilm	835:849	Candida biofilm	835:849	The 80% of Candida biofilm was inhibited and eradicated by CRG-AgNps at a concentration of ~300 μg/mL.					
33440209	2	18	theme	alternative	312:322	arg1	option					333:338	an excellent alternative remedial option	299:338	an excellent alternative remedial option	299:338	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	3	19	from	activity	638:645	arg1	terms					517:521	terms	517:521	terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix	517:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	4	20	theme	inhibitory	691:700	arg1	concentration					702:714	Minimum inhibitory concentration	683:714	Minimum inhibitory concentration	683:714	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	3	21	theme	AgNps	444:448	arg1	activity					380:387	the anti-biofilm activity	363:387	the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata	363:495	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	9	22	theme	biofilm	1302:1308	arg1	ability					1318:1324	the biofilm forming ability	1298:1324	the biofilm forming ability	1298:1324	CRG-AgNps also significantly inactivated the hydrolytic enzymes, thus hindering the biofilm forming ability.					
33440209	3	23	from	terms	517:521	arg1	activity					638:645	the activity	634:645	the activity of enzymes of extracellular matrix	634:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	23	from	terms	517:521	arg1	generation					572:581	reactive oxygen species (ROS) generation	542:581	reactive oxygen species (ROS) generation	542:581	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	23	from	terms	517:521	arg1	morphology					593:602	cellular morphology	584:602	cellular morphology	584:602	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	23	from	terms	517:521	arg1	composition					617:627	biochemical composition	605:627	biochemical composition	605:627	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	2	24	theme	excellent	302:310	arg1	option					333:338	an excellent alternative remedial option	299:338	an excellent alternative remedial option	299:338	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	1	25	theme	distressed	200:209	arg1	disease					211:217	substantially distressed disease	186:217	substantially distressed disease management for clinicians	186:243	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	3	26	theme	CRG	432:434	arg1	AgNps					444:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	6	27	theme	~300 μg/mL	915:924	arg1	concentration					898:910	a concentration	896:910	a concentration of ~300 μg/mL	896:924	The 80% of Candida biofilm was inhibited and eradicated by CRG-AgNps at a concentration of ~300 μg/mL.					
33440209	8	28	theme	Candida	1149:1155	arg1	ECM					1165:1167	Candida biofilm ECM	1149:1167	Candida biofilm ECM	1149:1167	Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content.					
33440209	4	29	theme	Minimum	683:689	arg1	concentration					702:714	Minimum inhibitory concentration	683:714	Minimum inhibitory concentration	683:714	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	1	30	theme	disease	211:217	arg1	management					219:228	substantially distressed disease management	186:228	substantially distressed disease management for clinicians	186:243	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	7	31	theme	morphological	978:990	arg1	damage					992:997	morphological damage	978:997	morphological damage	978:997	Microscopic studies indicate that CRG-AgNps caused morphological damage through membrane disruption and pore formation.					
33440209	1	32	theme	Global	110:115	arg1	crisis					131:136	Global antimicrobial crisis and advent	110:147	crisis	131:136	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	3	33	theme	kappa-carrageenan	413:429	arg1	AgNps					444:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	microwave irradiated kappa-carrageenan (CRG) capped AgNps	392:448	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	9	34	theme	forming	1310:1316	arg1	ability					1318:1324	the biofilm forming ability	1298:1324	the biofilm forming ability	1298:1324	CRG-AgNps also significantly inactivated the hydrolytic enzymes, thus hindering the biofilm forming ability.					
33440209	8	35	theme	ECM	1165:1167	arg1	composition					1134:1144	the composition	1130:1144	the composition of Candida biofilm ECM	1130:1167	Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content.					
33440209	3	36	from	effect	532:537	arg1	activity					638:645	the activity	634:645	the activity of enzymes of extracellular matrix	634:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	36	from	effect	532:537	arg1	generation					572:581	reactive oxygen species (ROS) generation	542:581	reactive oxygen species (ROS) generation	542:581	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	36	from	effect	532:537	arg1	morphology					593:602	cellular morphology	584:602	cellular morphology	584:602	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	36	from	effect	532:537	arg1	composition					617:627	biochemical composition	605:627	biochemical composition	605:627	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	1	37	theme	antimicrobial	117:129	arg1	crisis					131:136	Global antimicrobial crisis and advent	110:147	crisis	131:136	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	3	38	from	composition	617:627	arg1	terms					517:521	terms	517:521	terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix	517:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	0	39	theme	anti-biofilm	15:26	arg1	potency					28:34	the anti-biofilm potency	11:34	the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species	11:107	Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species.					
33440209	8	40	theme	biofilm	1157:1163	arg1	ECM					1165:1167	Candida biofilm ECM	1149:1167	Candida biofilm ECM	1149:1167	Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content.					
33440209	3	41	theme	matrix	675:680	arg1	enzymes					650:656	enzymes	650:656	enzymes of extracellular matrix	650:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	42	theme	cellular	584:591	arg1	morphology					593:602	cellular morphology	584:602	cellular morphology	584:602	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	10	43	theme	antifungal	1401:1410	arg1	CRG-AgNps					1377:1385	the CRG-AgNps	1373:1385	the CRG-AgNps	1373:1385	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	10	43	theme	antifungal	1401:1410	arg1	agents					1412:1417	potential antifungal agents	1391:1417	potential antifungal agents against Candida biofilms	1391:1442	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	3	44	theme	oxygen	551:556	arg1	ROS					567:569	ROS	567:569	ROS	567:569	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	44	theme	oxygen	551:556	arg1	species					558:564	reactive oxygen species	542:564	reactive oxygen species (ROS) generation	542:581	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	0	45	theme	kappa-carrageenan	39:55	arg1	nanoparticles					71:83	kappa-carrageenan capped silver nanoparticles	39:83	kappa-carrageenan capped silver nanoparticles	39:83	Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species.					
33440209	4	46	theme	CRG-AgNps	754:762	arg1	value					745:749	fungicidal concentration value	720:749	fungicidal concentration value	720:749	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	4	46	theme	CRG-AgNps	754:762	arg1	concentration					702:714	Minimum inhibitory concentration	683:714	Minimum inhibitory concentration	683:714	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	10	47	theme	potential	1391:1399	arg1	CRG-AgNps					1377:1385	the CRG-AgNps	1373:1385	the CRG-AgNps	1373:1385	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	10	47	theme	potential	1391:1399	arg1	agents					1412:1417	potential antifungal agents	1391:1417	potential antifungal agents against Candida biofilms	1391:1442	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	3	48	theme	Candida	458:464	arg1	albicans					466:473	Candida albicans	458:473	Candida albicans	458:473	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	0	49	theme	silver	64:69	arg1	nanoparticles					71:83	kappa-carrageenan capped silver nanoparticles	39:83	kappa-carrageenan capped silver nanoparticles	39:83	Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species.					
33440209	8	50	theme	carbohydrate	1187:1198	arg1	content					1209:1215	the carbohydrate and eDNA content	1183:1215	content	1209:1215	Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content.					
33440209	0	51	theme	nanoparticles	71:83	arg1	potency					28:34	the anti-biofilm potency	11:34	the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species	11:107	Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species.					
33440209	3	52	theme	biochemical	605:615	arg1	composition					617:627	biochemical composition	605:627	biochemical composition	605:627	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	0	53	theme	capped	57:62	arg1	nanoparticles					71:83	kappa-carrageenan capped silver nanoparticles	39:83	kappa-carrageenan capped silver nanoparticles	39:83	Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species.					
33440209	3	54	theme	current	348:354	arg1	study					356:360	the current study	344:360	the current study	344:360	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	2	55	theme	Biodegradable	246:258	arg1	AgNps					282:286	AgNps	282:286	AgNps	282:286	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	2	55	theme	Biodegradable	246:258	arg1	nanoparticles					267:279	Biodegradable silver nanoparticles	246:279	Biodegradable silver nanoparticles (AgNps)	246:287	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	2	56	theme	silver	260:265	arg1	AgNps					282:286	AgNps	282:286	AgNps	282:286	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	2	56	theme	silver	260:265	arg1	nanoparticles					267:279	Biodegradable silver nanoparticles	246:279	Biodegradable silver nanoparticles (AgNps)	246:287	Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option.					
33440209	3	57	theme	effect	532:537	arg1	terms					517:521	terms	517:521	terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix	517:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	8	58	theme	eDNA	1204:1207	arg1	content					1209:1215	the carbohydrate and eDNA content	1183:1215	content	1209:1215	Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content.					
33440209	4	59	theme	fungicidal	720:729	arg1	value					745:749	fungicidal concentration value	720:749	fungicidal concentration value	720:749	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	3	60	theme	extracellular	661:673	arg1	matrix					675:680	extracellular matrix	661:680	extracellular matrix	661:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	10	61	theme	Candida	1427:1433	arg1	biofilms					1435:1442	Candida biofilms	1427:1442	Candida biofilms	1427:1442	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	3	62	theme	enzymes	650:656	arg1	activity					638:645	the activity	634:645	the activity of enzymes of extracellular matrix	634:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	62	theme	enzymes	650:656	arg1	generation					572:581	reactive oxygen species (ROS) generation	542:581	reactive oxygen species (ROS) generation	542:581	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	62	theme	enzymes	650:656	arg1	morphology					593:602	cellular morphology	584:602	cellular morphology	584:602	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	62	theme	enzymes	650:656	arg1	composition					617:627	biochemical composition	605:627	biochemical composition	605:627	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	10	63	theme	fungal	1476:1481	arg1	biofilms					1483:1490	the fungal biofilms	1472:1490	the fungal biofilms	1472:1490	In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms.					
33440209	1	64	theme	fungal	167:172	arg1	strains					174:180	drug resistant fungal strains	152:180	drug resistant fungal strains	152:180	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	3	65	from	morphology	593:602	arg1	terms					517:521	terms	517:521	terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix	517:680	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	4	66	theme	Candida	777:783	arg1	spp					785:787	both Candida spp	772:787	both Candida spp	772:787	Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp.					
33440209	1	67	theme	resistant	157:165	arg1	strains					174:180	drug resistant fungal strains	152:180	drug resistant fungal strains	152:180	Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians.					
33440209	3	68	theme	Candida	480:486	arg1	glabrata					488:495	Candida glabrata	480:495	Candida glabrata	480:495	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	69	theme	anti-biofilm	367:378	arg1	activity					380:387	the anti-biofilm activity	363:387	the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata	363:495	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	3	70	theme	species	558:564	arg1	generation					572:581	reactive oxygen species (ROS) generation	542:581	reactive oxygen species (ROS) generation	542:581	In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix.					
33440209	7	71	theme	Microscopic	927:937	arg1	studies					939:945	Microscopic studies	927:945	Microscopic studies	927:945	Microscopic studies indicate that CRG-AgNps caused morphological damage through membrane disruption and pore formation.					
32533775	6	0	theme	RS4	827:829	arg1	parts					818:822	10-40 parts	812:822	10-40 parts of RS4	812:829	The substitution of 10-40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non-swelling properties of RS4.					
32533775	7	1	theme	protein	1107:1113	arg1	isolate					1115:1121	wheat protein isolate	1101:1121	wheat protein isolate	1101:1121	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	10	2	theme	=	1782:1782	arg1	RS/flour					1773:1780	RS/flour	1773:1780	RS/flour = 40/60	1773:1788	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	0	3	theme	wheat	172:176	arg1	starch					178:183	cross-linked phosphorylated RS4 wheat starch	140:183	cross-linked phosphorylated RS4 wheat starch	140:183	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	2	4	theme	winter	324:329	arg1	wheat					337:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	6	5	theme	parts	818:822	arg1	substitution					796:807	The substitution	792:807	The substitution of 10-40 parts of RS4 for flour	792:839	The substitution of 10-40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non-swelling properties of RS4.					
32533775	9	6	theme	wheat	1542:1546	arg1	flour					1548:1552	hard wheat flour	1537:1552	hard wheat flour	1537:1552	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	1	7	from	ingredient	271:280	arg1	products					298:305	various food products	285:305	various food products	285:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	3	8	theme	high	481:484	arg1	content					489:495	a high RS content	479:495	a high RS content	479:495	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	4	9	theme	protein	616:622	arg1	isolate					624:630	wheat protein isolate	610:630	wheat protein isolate	610:630	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	1	10	theme	BACKGROUND	186:195	arg1	starch					207:212	BACKGROUND Resistant starch	186:212	BACKGROUND Resistant starch (RS)	186:217	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	10	theme	BACKGROUND	186:195	arg1	ingredient					271:280	a functional ingredient	258:280	a functional ingredient in various food products	258:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	10	theme	BACKGROUND	186:195	arg1	RS					215:216	RS	215:216	RS	215:216	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	6	11	theme	cooked	868:873	arg1	noodles					875:881	cooked noodles	868:881	cooked noodles	868:881	The substitution of 10-40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non-swelling properties of RS4.					
32533775	9	12	theme	Asian	1565:1569	arg1	noodles					1571:1577	Asian noodles	1565:1577	Asian noodles	1565:1577	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	3	13	theme	HRW	498:500	arg1	flour					508:512	HRW wheat flour	498:512	HRW wheat flour	498:512	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	5	14	theme	HRW	767:769	arg1	flour					777:781	HRW wheat flour	767:781	HRW wheat flour	767:781	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	4	15	theme	wheat	594:598	arg1	gluten					600:605	Vital wheat gluten	588:605	Vital wheat gluten	588:605	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	0	16	theme	different	120:128	arg1	levels					130:135	different levels	120:135	different levels of cross-linked phosphorylated RS4 wheat starch	120:183	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	7	17	theme	replacement	1034:1044	arg1	parts					1021:1025	40 parts	1018:1025	40 parts of RS4 replacement	1018:1044	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	8	18	theme	endogenous	1347:1356	arg1	protein					1358:1364	endogenous protein	1347:1364	endogenous protein	1347:1364	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	11	19	theme	Chemical	1810:1817	arg1	Industry					1819:1826	Chemical Industry	1810:1826	Chemical Industry	1810:1826	© 2020 Society of Chemical Industry.					
32533775	10	20	with	supplementation	1709:1723	arg1	gluten					1742:1747	vital wheat gluten	1730:1747	vital wheat gluten in the composite flour (RS/flour = 40/60)	1730:1789	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	0	21	theme	phosphorylated	153:166	arg1	starch					178:183	cross-linked phosphorylated RS4 wheat starch	140:183	cross-linked phosphorylated RS4 wheat starch	140:183	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	3	22	theme	wheat	574:578	arg1	starch					580:585	cross-linked phosphorylated RS4 wheat starch	542:585	cross-linked phosphorylated RS4 wheat starch	542:585	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	8	23	theme	wheat	1375:1379	arg1	isolate					1389:1395	added wheat protein isolate	1369:1395	added wheat protein isolate	1369:1395	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	7	24	theme	wheat	1101:1105	arg1	isolate					1115:1121	wheat protein isolate	1101:1121	wheat protein isolate	1101:1121	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	3	25	theme	phosphorylated	555:568	arg1	starch					580:585	cross-linked phosphorylated RS4 wheat starch	542:585	cross-linked phosphorylated RS4 wheat starch	542:585	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	2	26	used	used	361:364	arg2	wheat					337:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	0	27	theme	hard	70:73	arg1	flour					92:96	hard red winter wheat flour	70:96	hard red winter wheat flour	70:96	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	4	28	theme	RS	700:701	arg1	addition					688:695	the addition	684:695	the addition of RS	684:701	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	0	29	theme	winter	79:84	arg1	flour					92:96	hard red winter wheat flour	70:96	hard red winter wheat flour	70:96	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	7	30	theme	parts	1070:1074	arg1	supplementation					1047:1061	supplementation	1047:1061	supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour	1047:1144	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	3	31	link	cross-linked	542:553	arg1	starch					580:585	cross-linked phosphorylated RS4 wheat starch	542:585	cross-linked phosphorylated RS4 wheat starch	542:585	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	8	32	theme	breaking	1428:1435	arg1	noodles					1412:1418	cooked noodles greater breaking force and distance	1405:1454	cooked noodles greater breaking force and distance	1405:1454	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	8	32	theme	breaking	1428:1435	arg1	force					1437:1441	greater breaking force	1420:1441	greater breaking force	1420:1441	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	7	33	theme	cooked	1197:1202	arg1	noodles					1204:1210	cooked noodles	1197:1210	cooked noodles	1197:1210	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	0	34	theme	textural	16:23	arg1	properties					25:34	textural properties	16:34	textural properties	16:34	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	1	35	theme	functional	260:269	arg1	starch					207:212	BACKGROUND Resistant starch	186:212	BACKGROUND Resistant starch (RS)	186:217	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	35	theme	functional	260:269	arg1	ingredient					271:280	a functional ingredient	258:280	a functional ingredient in various food products	258:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	36	used	used	250:253	arg2	RS					215:216	RS	215:216	RS	215:216	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	36	used	used	250:253	arg2	starch					207:212	BACKGROUND Resistant starch	186:212	BACKGROUND Resistant starch (RS)	186:217	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	36	used	used	250:253	arg2	ingredient					271:280	a functional ingredient	258:280	a functional ingredient in various food products	258:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	0	37	link	cross-linked	140:151	arg1	starch					178:183	cross-linked phosphorylated RS4 wheat starch	140:183	cross-linked phosphorylated RS4 wheat starch	140:183	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	8	38	theme	vital	1288:1292	arg1	gluten					1300:1305	Supplemental vital wheat gluten	1275:1305	Supplemental vital wheat gluten	1275:1305	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	5	39	theme	maximum	716:722	arg1	level					736:740	The maximum recommended level	712:740	The maximum recommended level of RS4 starch to replace HRW wheat flour	712:781	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	5	39	theme	maximum	716:722	arg1	%					789:789	40%	787:789	40%	787:789	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	2	40	theme	strong	413:418	arg1	network					427:433	its strong gluten network	409:433	its strong gluten network	409:433	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	10	41	theme	vital	1730:1734	arg1	gluten					1742:1747	vital wheat gluten	1730:1747	vital wheat gluten in the composite flour (RS/flour = 40/60)	1730:1789	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	2	42	theme	red	320:322	arg1	wheat					337:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	0	43	theme	salted	45:50	arg1	noodles					52:58	white salted noodles	39:58	white salted noodles made with hard red winter wheat flour	39:96	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	1	44	theme	food	293:296	arg1	products					298:305	various food products	285:305	various food products	285:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	2	45	theme	Kansas	308:313	arg1	wheat					337:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	7	46	dep	hardness	1167:1174	arg1	the					1163:1165	the	1163:1165	the	1163:1165	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	8	47	theme	protein	1326:1332	arg1	network					1334:1340	a thicker protein network	1316:1340	a thicker protein network	1316:1340	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	1	48	theme	health	223:228	arg1	benefits					230:237	health benefits	223:237	health benefits	223:237	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	0	49	theme	noodles	52:58	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	0	49	theme	noodles	52:58	arg1	properties					25:34	textural properties	16:34	textural properties	16:34	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	6	50	theme	non-swelling	983:994	arg1	properties					996:1005	the non-swelling properties	979:1005	the non-swelling properties of RS4	979:1012	The substitution of 10-40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non-swelling properties of RS4.					
32533775	10	51	dep	flour	1766:1770	arg1	RS/flour					1773:1780	RS/flour	1773:1780	RS/flour = 40/60	1773:1788	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	7	52	theme	wheat	1085:1089	arg1	gluten					1091:1096	vital wheat gluten	1079:1096	vital wheat gluten	1079:1096	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	0	53	theme	starch	178:183	arg1	levels					130:135	different levels	120:135	different levels of cross-linked phosphorylated RS4 wheat starch	120:183	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	3	54	theme	white	453:457	arg1	noodles					466:472	Asian white salted noodles	447:472	Asian white salted noodles with a high RS content	447:495	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	10	55	from	gluten	1742:1747	arg1	flour					1766:1770	the composite flour	1752:1770	the composite flour (RS/flour = 40/60)	1752:1789	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	9	56	used	used	1481:1484	arg2	ingredient					1502:1511	a functional ingredient	1489:1511	a functional ingredient	1489:1511	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	9	56	used	used	1481:1484	arg2	CONCLUSION					1457:1466	CONCLUSION RS4	1457:1470	CONCLUSION RS4	1457:1470	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	7	57	theme	isolate	1115:1121	arg1	parts					1070:1074	2-8 parts	1066:1074	2-8 parts of vital wheat gluten or wheat protein isolate	1066:1121	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	3	58	with	noodles	466:472	arg1	content					489:495	a high RS content	479:495	a high RS content	479:495	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	3	59	theme	RS	486:487	arg1	content					489:495	a high RS content	479:495	a high RS content	479:495	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	9	60	theme	hard	1537:1540	arg1	flour					1548:1552	hard wheat flour	1537:1552	hard wheat flour	1537:1552	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	9	61	theme	flour	1548:1552	arg1	flour					1548:1552	hard wheat flour	1537:1552	hard wheat flour	1537:1552	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	9	61	theme	flour	1548:1552	arg1	%					1532:1532	up to 40%	1524:1532	up to 40% of hard wheat flour	1524:1552	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	7	62	theme	gluten	1091:1096	arg1	parts					1070:1074	2-8 parts	1066:1074	2-8 parts of vital wheat gluten or wheat protein isolate	1066:1121	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	3	63	theme	wheat	502:506	arg1	flour					508:512	HRW wheat flour	498:512	HRW wheat flour	498:512	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	4	64	theme	wheat	610:614	arg1	isolate					624:630	wheat protein isolate	610:630	wheat protein isolate	610:630	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	10	65	theme	noodles	1655:1661	arg1	extensibility					1631:1643	The extensibility	1627:1643	The extensibility of cooked noodles with high RS4	1627:1675	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	1	66	theme	Resistant	197:205	arg1	starch					207:212	BACKGROUND Resistant starch	186:212	BACKGROUND Resistant starch (RS)	186:217	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	66	theme	Resistant	197:205	arg1	ingredient					271:280	a functional ingredient	258:280	a functional ingredient in various food products	258:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	66	theme	Resistant	197:205	arg1	RS					215:216	RS	215:216	RS	215:216	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	5	67	dep	RESULTS	704:710	arg1	level					736:740	The maximum recommended level	712:740	The maximum recommended level of RS4 starch to replace HRW wheat flour	712:781	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	5	67	dep	RESULTS	704:710	arg1	%					789:789	40%	787:789	40%	787:789	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	2	68	theme	hard	315:318	arg1	wheat					337:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	5	69	theme	wheat	771:775	arg1	flour					777:781	HRW wheat flour	767:781	HRW wheat flour	767:781	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	4	70	theme	Vital	588:592	arg1	gluten					600:605	Vital wheat gluten	588:605	Vital wheat gluten	588:605	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	0	71	theme	cross-linked	140:151	arg1	starch					178:183	cross-linked phosphorylated RS4 wheat starch	140:183	cross-linked phosphorylated RS4 wheat starch	140:183	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	1	72	contain	has	219:221	arg1	starch					207:212	BACKGROUND Resistant starch	186:212	BACKGROUND Resistant starch (RS)	186:217	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	72	contain	has	219:221	arg1	ingredient					271:280	a functional ingredient	258:280	a functional ingredient in various food products	258:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	72	contain	has	219:221	arg2	benefits					230:237	health benefits	223:237	health benefits	223:237	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	1	72	contain	has	219:221	arg1	RS					215:216	RS	215:216	RS	215:216	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	8	73	theme	added	1369:1373	arg1	isolate					1389:1395	added wheat protein isolate	1369:1395	added wheat protein isolate	1369:1395	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	10	74	theme	cooked	1648:1653	arg1	noodles					1655:1661	cooked noodles	1648:1661	cooked noodles with high RS4	1648:1675	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	8	75	theme	protein	1381:1387	arg1	isolate					1389:1395	added wheat protein isolate	1369:1395	added wheat protein isolate	1369:1395	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	4	76	theme	textural	660:667	arg1	changes					669:675	textural changes	660:675	textural changes due to the addition of RS	660:701	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	7	77	theme	composite	1130:1138	arg1	flour					1140:1144	the composite flour	1126:1144	the composite flour	1126:1144	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	3	78	theme	cross-linked	542:553	arg1	starch					580:585	cross-linked phosphorylated RS4 wheat starch	542:585	cross-linked phosphorylated RS4 wheat starch	542:585	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	7	79	theme	vital	1079:1083	arg1	gluten					1091:1096	vital wheat gluten	1079:1096	vital wheat gluten	1079:1096	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	0	80	theme	red	75:77	arg1	flour					92:96	hard red winter wheat flour	70:96	hard red winter wheat flour	70:96	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	10	81	with	noodles	1655:1661	arg1	RS4					1673:1675	RS4	1673:1675	RS4	1673:1675	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	8	82	theme	cooked	1405:1410	arg1	distance					1447:1454	distance	1447:1454	distance	1447:1454	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	8	82	theme	cooked	1405:1410	arg1	noodles					1412:1418	cooked noodles greater breaking force and distance	1405:1454	cooked noodles greater breaking force and distance	1405:1454	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	8	82	theme	cooked	1405:1410	arg1	force					1437:1441	greater breaking force	1420:1441	greater breaking force	1420:1441	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	0	83	theme	wheat	86:90	arg1	flour					92:96	hard red winter wheat flour	70:96	hard red winter wheat flour	70:96	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	9	84	dep	40	1530:1531	arg1	to					1527:1528	to	1527:1528	to	1527:1528	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	8	85	theme	greater	1420:1426	arg1	noodles					1412:1418	cooked noodles greater breaking force and distance	1405:1454	cooked noodles greater breaking force and distance	1405:1454	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	8	85	theme	greater	1420:1426	arg1	force					1437:1441	greater breaking force	1420:1441	greater breaking force	1420:1441	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	7	86	theme	noodles	1204:1210	arg1	extensibility					1180:1192	extensibility	1180:1192	extensibility	1180:1192	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	7	86	theme	noodles	1204:1210	arg1	hardness					1167:1174	hardness	1167:1174	hardness	1167:1174	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	7	87	from	supplementation	1047:1061	arg1	flour					1140:1144	the composite flour	1126:1144	the composite flour	1126:1144	At 40 parts of RS4 replacement, supplementation of 2-8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected.					
32533775	4	88	theme	due	677:679	arg1	changes					669:675	textural changes	660:675	textural changes due to the addition of RS	660:701	Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.					
32533775	5	89	theme	starch	749:754	arg1	level					736:740	The maximum recommended level	712:740	The maximum recommended level of RS4 starch to replace HRW wheat flour	712:781	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	5	89	theme	starch	749:754	arg1	%					789:789	40%	787:789	40%	787:789	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	6	90	from	hardness	856:863	arg1	noodles					875:881	cooked noodles	868:881	cooked noodles	868:881	The substitution of 10-40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non-swelling properties of RS4.					
32533775	2	91	theme	making	376:381	arg1	bread					370:374	bread making	370:381	bread making	370:381	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	0	92	theme	white	39:43	arg1	noodles					52:58	white salted noodles	39:58	white salted noodles made with hard red winter wheat flour	39:96	Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross-linked phosphorylated RS4 wheat starch.					
32533775	2	93	theme	gluten	420:425	arg1	network					427:433	its strong gluten network	409:433	its strong gluten network	409:433	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	10	94	dep	=	1782:1782	arg1	40/60					1784:1788	40/60	1784:1788	40/60	1784:1788	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	10	95	theme	wheat	1736:1740	arg1	gluten					1742:1747	vital wheat gluten	1730:1747	vital wheat gluten in the composite flour (RS/flour = 40/60)	1730:1789	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	8	96	theme	Supplemental	1275:1286	arg1	gluten					1300:1305	Supplemental vital wheat gluten	1275:1305	Supplemental vital wheat gluten	1275:1305	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	5	97	theme	recommended	724:734	arg1	level					736:740	The maximum recommended level	712:740	The maximum recommended level of RS4 starch to replace HRW wheat flour	712:781	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	5	97	theme	recommended	724:734	arg1	%					789:789	40%	787:789	40%	787:789	RESULTS The maximum recommended level of RS4 starch to replace HRW wheat flour was 40%.					
32533775	1	98	theme	various	285:291	arg1	products					298:305	various food products	285:305	various food products	285:305	BACKGROUND Resistant starch (RS) has health benefits and can be used as a functional ingredient in various food products.					
32533775	9	99	theme	functional	1491:1500	arg1	CONCLUSION					1457:1466	CONCLUSION RS4	1457:1470	CONCLUSION RS4	1457:1470	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	9	99	theme	functional	1491:1500	arg1	ingredient					1502:1511	a functional ingredient	1489:1511	a functional ingredient	1489:1511	CONCLUSION RS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking.					
32533775	8	100	theme	wheat	1294:1298	arg1	gluten					1300:1305	Supplemental vital wheat gluten	1275:1305	Supplemental vital wheat gluten	1275:1305	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	3	101	theme	salted	459:464	arg1	noodles					466:472	Asian white salted noodles	447:472	Asian white salted noodles with a high RS content	447:495	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	3	102	theme	Asian	447:451	arg1	noodles					466:472	Asian white salted noodles	447:472	Asian white salted noodles with a high RS content	447:495	To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross-linked phosphorylated RS4 wheat starch.					
32533775	6	103	theme	RS4	1010:1012	arg1	properties					996:1005	the non-swelling properties	979:1005	the non-swelling properties of RS4	979:1012	The substitution of 10-40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non-swelling properties of RS4.					
32533775	10	104	theme	composite	1756:1764	arg1	flour					1766:1770	the composite flour	1752:1770	the composite flour (RS/flour = 40/60)	1752:1789	The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60).					
32533775	8	105	theme	thicker	1318:1324	arg1	network					1334:1340	a thicker protein network	1316:1340	a thicker protein network	1316:1340	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	2	106	theme	HRW	332:334	arg1	wheat					337:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat	308:341	Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network.					
32533775	8	107	dep	noodles	1412:1418	arg1	distance					1447:1454	distance	1447:1454	distance	1447:1454	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	8	107	dep	noodles	1412:1418	arg1	noodles					1412:1418	cooked noodles greater breaking force and distance	1405:1454	cooked noodles greater breaking force and distance	1405:1454	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
32533775	8	107	dep	noodles	1412:1418	arg1	force					1437:1441	greater breaking force	1420:1441	greater breaking force	1420:1441	Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.					
33189954	10	0	theme	hyaluronic	1901:1910	arg1	acid					1912:1915	hyaluronic acid	1901:1915	hyaluronic acid	1901:1915	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	6	1	theme	ultimate	882:889	arg1	MPa					877:879	Young's modulus=19±4 MPa	856:879	Young's modulus=19±4 MPa	856:879	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	1	theme	ultimate	882:889	arg1	MPa					914:916	ultimate tensile stress=9.6±1.7 MPa	882:916	ultimate tensile stress=9.6±1.7 MPa	882:916	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	1	2	theme	post-operative	165:178	arg1	adhesions					194:202	post-operative peritendinous adhesions	165:202	post-operative peritendinous adhesions	165:202	The repair of tendon injuries is often compromised by post-operative peritendinous adhesions.					
33189954	9	3	theme	rabbit	1660:1665	arg1	growth					1679:1684	synoviocyte growth	1667:1684	synoviocyte growth	1667:1684	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	7	4	theme	waste	1322:1326	arg1	diffusion					1295:1303	diffusion	1295:1303	diffusion of nutrients and waste	1295:1326	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	3	5	from	present	367:373	arg1	available					394:402	available	394:402	available	394:402	At present, no such system is available for routine use in clinical practice.					
33189954	8	6	theme	lubrication	1399:1409	arg1	properties					1411:1420	good lubrication properties	1394:1420	good lubrication properties	1394:1420	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	7	7	theme	nutrients	1308:1316	arg1	diffusion					1295:1303	diffusion	1295:1303	diffusion of nutrients and waste	1295:1326	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	10	8	theme	gliding	1988:1994	arg1	environment					1996:2006	the tendon gliding environment	1977:2006	the tendon gliding environment	1977:2006	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	1	9	theme	tendon	125:130	arg1	injuries					132:139	tendon injuries	125:139	tendon injuries	125:139	The repair of tendon injuries is often compromised by post-operative peritendinous adhesions.					
33189954	7	10	theme	small	1186:1190	arg1	pores					1192:1196	small pores	1186:1196	small pores (< 3 μm)	1186:1205	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	7	10	theme	small	1186:1190	arg1	μm					1203:1204	< 3 μm	1199:1204	< 3 μm	1199:1204	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	9	11	theme	culture	1723:1729	arg1	days					1712:1715	28 days	1709:1715	28 days of 3D culture	1709:1729	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	4	12	theme	bilayer	480:486	arg1	membrane					488:495	a bilayer membrane	478:495	a bilayer membrane	478:495	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	8	13	theme	porcine	1463:1469	arg1	tendon					1471:1476	porcine tendon	1463:1476	porcine tendon	1463:1476	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	0	14	theme	tendon	87:92	arg1	sheath					103:108	the tendon synovial sheath	83:108	the tendon synovial sheath	83:108	Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath.					
33189954	0	15	theme	sheath	103:108	arg1	function					71:78	function	71:78	function	71:78	Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath.					
33189954	0	15	theme	sheath	103:108	arg1	structure					57:65	structure	57:65	structure	57:65	Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath.					
33189954	9	16	theme	specific	1762:1769	arg1	components					1778:1787	specific matrix components	1762:1787	specific matrix components	1762:1787	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	9	16	theme	specific	1762:1769	arg1	acid					1814:1817	hyaluronic acid	1803:1817	hyaluronic acid	1803:1817	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	7	17	theme	extrinsic	1258:1266	arg1	healing					1268:1274	extrinsic healing	1258:1274	extrinsic healing	1258:1274	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	9	18	theme	components	1778:1787	arg1	production					1748:1757	cell production	1743:1757	cell production of specific matrix components, particularly hyaluronic acid	1743:1817	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	0	19	theme	cell-hydrogel-fibre	14:32	arg1	composite					34:42	a cell-hydrogel-fibre composite	12:42	a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath	12:108	Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath.					
33189954	6	20	theme	Young	856:860	arg1	modulus=19±4					864:875	Young's modulus=19±4	856:875	Young's modulus=19±4 MPa	856:879	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	3	21	theme	routine	408:414	arg1	use					416:418	routine use	408:418	routine use in clinical practice	408:439	At present, no such system is available for routine use in clinical practice.					
33189954	7	22	theme	fibroblast	1224:1233	arg1	infiltration					1235:1246	fibroblast infiltration	1224:1246	fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste	1224:1326	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	8	23	theme	gliding	1531:1537	arg1	restoration					1509:1519	restoration	1509:1519	restoration of tendon gliding	1509:1537	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	10	24	theme	Synoviocyte-laden	1820:1836	arg1	hydrogel					1846:1853	Synoviocyte-laden peptide hydrogel	1820:1853	Synoviocyte-laden peptide hydrogel	1820:1853	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	5	25	theme	synovial	774:781	arg1	regeneration					790:801	synovial sheath regeneration	774:801	synovial sheath regeneration	774:801	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	6	26	theme	adequate	824:831	arg1	properties					844:853	adequate mechanical properties	824:853	adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N)	824:941	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	10	27	theme	endogenous	1876:1885	arg1	production					1887:1896	a sustained endogenous production	1864:1896	a sustained endogenous production of hyaluronic acid	1864:1915	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	5	28	theme	tendon	733:738	arg1	lubrication					740:750	tendon lubrication	733:750	tendon lubrication	733:750	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	8	29	theme	layer	1381:1385	arg1	incorporation					1359:1371	incorporation	1359:1371	incorporation of SAPH layer	1359:1385	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	9	30	theme	activity	1604:1611	arg1	tests					1613:1617	viability and metabolic activity tests	1580:1617	viability and metabolic activity tests	1580:1617	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	4	31	theme	nanofibrous	509:519	arg1	mesh					560:563	a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh	507:563	a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh	507:563	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	10	32	theme	acid	1912:1915	arg1	production					1887:1896	a sustained endogenous production	1864:1896	a sustained endogenous production of hyaluronic acid	1864:1915	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	6	33	theme	tensile	891:897	arg1	MPa					877:879	Young's modulus=19±4 MPa	856:879	Young's modulus=19±4 MPa	856:879	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	33	theme	tensile	891:897	arg1	MPa					914:916	ultimate tensile stress=9.6±1.7 MPa	882:916	ultimate tensile stress=9.6±1.7 MPa	882:916	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	4	34	with	laden	621:625	arg1	synoviocytes					639:650	type-B synoviocytes	632:650	type-B synoviocytes	632:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	4	35	theme	electrospun	548:558	arg1	mesh					560:563	a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh	507:563	a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh	507:563	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	10	36	theme	anti-friction	1931:1943	arg1	layer					1945:1949	an anti-friction layer	1928:1949	an anti-friction layer that potentially restores the tendon gliding environment	1928:2006	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	2	37	theme	surrounding	276:286	arg1	tissue					288:293	the surrounding tissue	272:293	the surrounding tissue	272:293	Placing a physical barrier at the interface between the tendon and the surrounding tissue could potentially solve this problem by reducing adhesion formation.					
33189954	6	38	theme	modulus=19±4	864:875	arg1	MPa					877:879	Young's modulus=19±4 MPa	856:879	Young's modulus=19±4 MPa	856:879	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	38	theme	modulus=19±4	864:875	arg1	N					940:940	failure load=0.5±0.1 N	919:940	failure load=0.5±0.1 N	919:940	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	38	theme	modulus=19±4	864:875	arg1	MPa					914:916	ultimate tensile stress=9.6±1.7 MPa	882:916	ultimate tensile stress=9.6±1.7 MPa	882:916	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	4	39	theme	laden	621:625	arg1	layer					572:576	a layer	570:576	a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes	570:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	1	40	theme	peritendinous	180:192	arg1	adhesions					194:202	post-operative peritendinous adhesions	165:202	post-operative peritendinous adhesions	165:202	The repair of tendon injuries is often compromised by post-operative peritendinous adhesions.					
33189954	4	41	theme	type-B	632:637	arg1	synoviocytes					639:650	type-B synoviocytes	632:650	type-B synoviocytes	632:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	9	42	theme	synoviocyte	1667:1677	arg1	growth					1679:1684	synoviocyte growth	1667:1684	synoviocyte growth	1667:1684	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	8	43	theme	good	1394:1397	arg1	properties					1411:1420	good lubrication properties	1394:1420	good lubrication properties	1394:1420	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	4	44	theme	hydrogel	605:612	arg1	laden					621:625	self-assembling peptide hydrogel (SAPH) laden	581:625	self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes	581:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	7	45	with	mesh	1151:1154	arg1	fibres					1175:1180	nanosized PCL fibres	1161:1180	nanosized PCL fibres	1161:1180	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	7	45	with	mesh	1151:1154	arg1	pores					1192:1196	small pores	1186:1196	small pores (< 3 μm)	1186:1205	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	7	45	with	mesh	1151:1154	arg1	μm					1203:1204	< 3 μm	1199:1204	< 3 μm	1199:1204	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	4	46	theme	SAPH	615:618	arg1	laden					621:625	self-assembling peptide hydrogel (SAPH) laden	581:625	self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes	581:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	5	47	theme	anti-adhesion	691:703	arg1	membrane					666:673	This bilayer membrane	653:673	This bilayer membrane	653:673	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	5	47	theme	anti-adhesion	691:703	arg1	system					705:710	an anti-adhesion system	688:710	an anti-adhesion system capable of restoring tendon lubrication	688:750	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	4	48	theme	poly	521:524	arg1	mesh					560:563	a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh	507:563	a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh	507:563	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	4	49	theme	self-assembling	581:595	arg1	laden					621:625	self-assembling peptide hydrogel (SAPH) laden	581:625	self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes	581:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	6	50	theme	failure	919:925	arg1	MPa					877:879	Young's modulus=19±4 MPa	856:879	Young's modulus=19±4 MPa	856:879	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	50	theme	failure	919:925	arg1	N					940:940	failure load=0.5±0.1 N	919:940	failure load=0.5±0.1 N	919:940	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	10	51	theme	tendon	1981:1986	arg1	environment					1996:2006	the tendon gliding environment	1977:2006	the tendon gliding environment	1977:2006	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	5	52	theme	bilayer	658:664	arg1	membrane					666:673	This bilayer membrane	653:673	This bilayer membrane	653:673	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	5	52	theme	bilayer	658:664	arg1	system					705:710	an anti-adhesion system	688:710	an anti-adhesion system capable of restoring tendon lubrication	688:750	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	1	53	theme	injuries	132:139	arg1	repair					115:120	The repair	111:120	The repair of tendon injuries	111:139	The repair of tendon injuries is often compromised by post-operative peritendinous adhesions.					
33189954	9	54	theme	3D	1720:1721	arg1	culture					1723:1729	3D culture	1720:1729	3D culture	1720:1729	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	4	55	theme	membrane	488:495	arg1	development					463:473	the development	459:473	the development of a bilayer membrane	459:495	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	7	56	theme	nanosized	1161:1169	arg1	fibres					1175:1180	nanosized PCL fibres	1161:1180	nanosized PCL fibres	1161:1180	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	7	57	theme	PCL	1171:1173	arg1	fibres					1175:1180	nanosized PCL fibres	1161:1180	nanosized PCL fibres	1161:1180	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	0	58	theme	synovial	94:101	arg1	sheath					103:108	the tendon synovial sheath	83:108	the tendon synovial sheath	83:108	Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath.					
33189954	3	59	from	use	416:418	arg1	practice					432:439	clinical practice	423:439	clinical practice	423:439	At present, no such system is available for routine use in clinical practice.					
33189954	9	60	theme	cell	1743:1746	arg1	production					1748:1757	cell production	1743:1757	cell production of specific matrix components, particularly hyaluronic acid	1743:1817	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	5	61	theme	capable	712:718	arg1	membrane					666:673	This bilayer membrane	653:673	This bilayer membrane	653:673	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	5	61	theme	capable	712:718	arg1	system					705:710	an anti-adhesion system	688:710	an anti-adhesion system capable of restoring tendon lubrication	688:750	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	5	62	theme	sheath	783:788	arg1	regeneration					790:801	synovial sheath regeneration	774:801	synovial sheath regeneration	774:801	This bilayer membrane would act as an anti-adhesion system capable of restoring tendon lubrication, while assisting with synovial sheath regeneration.					
33189954	9	63	theme	matrix	1771:1776	arg1	components					1778:1787	specific matrix components	1762:1787	specific matrix components	1762:1787	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	9	63	theme	matrix	1771:1776	arg1	acid					1814:1817	hyaluronic acid	1803:1817	hyaluronic acid	1803:1817	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	3	64	theme	clinical	423:430	arg1	practice					432:439	clinical practice	423:439	clinical practice	423:439	At present, no such system is available for routine use in clinical practice.					
33189954	7	65	dep	infiltration	1235:1246	arg1	allowing					1286:1293	allowing	1286:1293	still allowing diffusion of nutrients and waste	1280:1326	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	7	65	dep	infiltration	1235:1246	arg1	impede					1251:1256	impede	1251:1256	to impede extrinsic healing	1248:1274	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	6	66	theme	frictional	1020:1029	arg1	forces					1031:1036	the frictional forces	1016:1036	the frictional forces generated on the tendon's surface during movement (~0.3 N)	1016:1095	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	3	67	theme	such	379:382	arg1	system					384:389	no such system	376:389	no such system	376:389	At present, no such system is available for routine use in clinical practice.					
33189954	8	68	theme	tendon	1524:1529	arg1	gliding					1531:1537	tendon gliding	1524:1537	tendon gliding	1524:1537	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	7	69	theme	Morphological	1098:1110	arg1	analysis					1112:1119	Morphological analysis	1098:1119	Morphological analysis	1098:1119	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	0	70	dep	structure	57:65	arg1	the					53:55	the	53:55	the	53:55	Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath.					
33189954	9	71	theme	hyaluronic	1803:1812	arg1	components					1778:1787	specific matrix components	1762:1787	specific matrix components	1762:1787	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	9	71	theme	hyaluronic	1803:1812	arg1	acid					1814:1817	hyaluronic acid	1803:1817	hyaluronic acid	1803:1817	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	6	72	dep	properties	844:853	arg1	MPa					877:879	Young's modulus=19±4 MPa	856:879	Young's modulus=19±4 MPa	856:879	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	72	dep	properties	844:853	arg1	N					940:940	failure load=0.5±0.1 N	919:940	failure load=0.5±0.1 N	919:940	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	6	72	dep	properties	844:853	arg1	MPa					914:916	ultimate tensile stress=9.6±1.7 MPa	882:916	ultimate tensile stress=9.6±1.7 MPa	882:916	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	10	73	theme	peptide	1838:1844	arg1	hydrogel					1846:1853	Synoviocyte-laden peptide hydrogel	1820:1853	Synoviocyte-laden peptide hydrogel	1820:1853	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	8	74	theme	Rheological	1329:1339	arg1	tests					1341:1345	Rheological tests	1329:1345	Rheological tests	1329:1345	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
33189954	7	75	theme	mesh	1151:1154	arg1	generation					1135:1144	the generation	1131:1144	the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste	1131:1326	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	6	76	theme	mechanical	833:842	arg1	properties					844:853	adequate mechanical properties	824:853	adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N)	824:941	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	2	77	theme	physical	215:222	arg1	barrier					224:230	a physical barrier	213:230	a physical barrier	213:230	Placing a physical barrier at the interface between the tendon and the surrounding tissue could potentially solve this problem by reducing adhesion formation.					
33189954	2	78	theme	adhesion	344:351	arg1	formation					353:361	adhesion formation	344:361	adhesion formation	344:361	Placing a physical barrier at the interface between the tendon and the surrounding tissue could potentially solve this problem by reducing adhesion formation.					
33189954	9	79	theme	viability	1580:1588	arg1	tests					1613:1617	viability and metabolic activity tests	1580:1617	viability and metabolic activity tests	1580:1617	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	10	80	theme	sustained	1866:1874	arg1	production					1887:1896	a sustained endogenous production	1864:1896	a sustained endogenous production of hyaluronic acid	1864:1915	Synoviocyte-laden peptide hydrogel promoted a sustained endogenous production of hyaluronic acid, providing an anti-friction layer that potentially restores the tendon gliding environment.					
33189954	6	81	theme	PCL	808:810	arg1	mesh					812:815	The PCL mesh	804:815	The PCL mesh	804:815	The PCL mesh showed adequate mechanical properties (Young's modulus=19±4 MPa, ultimate tensile stress=9.6±1.7 MPa, failure load=0.5±0.1 N), indicating that the membrane is easy to handle and capable to withstand the frictional forces generated on the tendon's surface during movement (~0.3 N).					
33189954	9	82	theme	metabolic	1594:1602	arg1	activity					1604:1611	metabolic activity	1594:1611	metabolic activity	1594:1611	Moreover, viability and metabolic activity tests indicated that the SAPH was conducive to rabbit synoviocyte growth and proliferation over 28 days of 3D culture, sustaining cell production of specific matrix components, particularly hyaluronic acid.					
33189954	7	83	theme	<	1199:1199	arg1	pores					1192:1196	small pores	1186:1196	small pores (< 3 μm)	1186:1205	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	7	83	theme	<	1199:1199	arg1	μm					1203:1204	< 3 μm	1199:1204	< 3 μm	1199:1204	Morphological analysis confirmed the generation of a mesh with nanosized PCL fibres and small pores (< 3 μm), which prevented fibroblast infiltration to impede extrinsic healing but still allowing diffusion of nutrients and waste.					
33189954	4	84	theme	peptide	597:603	arg1	laden					621:625	self-assembling peptide hydrogel (SAPH) laden	581:625	self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes	581:650	Here, we propose the development of a bilayer membrane combining a nanofibrous poly(ε-caprolactone) (PCL) electrospun mesh with a layer of self-assembling peptide hydrogel (SAPH) laden with type-B synoviocytes.					
33189954	8	85	theme	SAPH	1376:1379	arg1	layer					1381:1385	SAPH layer	1376:1385	SAPH layer	1376:1385	Rheological tests showed that incorporation of SAPH layer allows good lubrication properties when the membrane is articulated against porcine tendon or hypodermis, suggesting that restoration of tendon gliding is possible upon implantation.					
31937642	14	0	theme	glycoamino	2469:2478	arg1	fucosyl-α-1,6-N-GlcNAc-Asn					2485:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	0	theme	glycoamino	2469:2478	arg1	present					2522:2528	present	2522:2528	present	2522:2528	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	4	1	theme	alfR2	878:882	arg1	genes					986:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	3	2	gly	N-glycoproteins	672:686	arg1	N-glycoproteins					672:686	mammalian N-glycoproteins	662:686	mammalian N-glycoproteins	662:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	2	3	theme	acids	375:379	arg1	role					337:340	the role	333:340	the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth	333:418	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	4	4	theme	transcriptional	885:899	arg1	alfR2					878:882	alfR2	878:882	alfR2 (transcriptional regulator)	878:910	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	4	4	theme	transcriptional	885:899	arg1	regulator					901:909	transcriptional regulator	885:909	transcriptional regulator	885:909	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	14	5	from	N-glycoproteins	2554:2568	arg1	fucosyl-α-1,6-N-GlcNAc-Asn					2485:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	5	from	N-glycoproteins	2554:2568	arg1	present					2522:2528	present	2522:2528	present	2522:2528	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	15	6	theme	host	2658:2661	arg1	bacteria					2687:2694	the host and commensal/probiotic bacteria	2654:2694	bacteria	2687:2694	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	13	7	theme	mucosal	2261:2267	arg1	surfaces					2269:2276	the mucosal surfaces	2257:2276	the mucosal surfaces	2257:2276	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	4	8	theme	aspartate	850:858	arg1	4-decarboxylase					860:874	aspartate 4-decarboxylase	850:874	aspartate 4-decarboxylase	850:874	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	4	8	theme	aspartate	850:858	arg1	asdA					844:847	the divergently oriented asdA	819:847	the divergently oriented asdA (aspartate 4-decarboxylase)	819:875	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	2	9	theme	bacterial	403:411	arg1	growth					413:418	bacterial growth	403:418	bacterial growth	403:418	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	3	10	gly	core-fucosylated	631:646	arg1	structures					648:657	the core-fucosylated structures	627:657	the core-fucosylated structures of mammalian N-glycoproteins	627:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	14	11	from	mammalians	2575:2584	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	6	12	theme	alf-2	1110:1114	arg1	genes					1116:1120	The alf-2 genes	1106:1120	The alf-2 genes	1106:1120	The alf-2 genes are induced by 6'FN-Asn, but not by its glycan moiety, via the AlfR2 regulator.					
31937642	15	13	theme	molecular	2623:2631	arg1	interactions					2633:2644	the molecular interactions	2619:2644	the molecular interactions between the host and commensal/probiotic bacteria	2619:2694	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	11	14	theme	enormous	2032:2039	arg1	impact					2041:2046	an enormous impact	2029:2046	an enormous impact on human health	2029:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	3	15	theme	acid	566:569	arg1	6'FN-Asn					599:606	6'FN-Asn	599:606	6'FN-Asn	599:606	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	15	theme	acid	566:569	arg1	fucosyl-α-1,6-N-GlcNAc-Asn					571:596	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	551:596	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn)	551:607	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	15	theme	acid	566:569	arg1	constituent					612:622	a constituent	610:622	a constituent of the core-fucosylated structures of mammalian N-glycoproteins	610:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	12	16	theme	commensal	2089:2097	arg1	bacteria					2099:2106	commensal bacteria	2089:2106	commensal bacteria	2089:2106	The mechanisms enabling commensal bacteria and administered probiotics to colonize the gut remain largely unknown.					
31937642	8	17	theme	mutant	1374:1379	arg1	background					1381:1390	this mutant background	1369:1390	this mutant background	1369:1390	However, GlcNAc-Asn did not support growth in this mutant background, indicating that the presence of a 6'-fucose moiety is crucial for substrate transport via AlfH.					
31937642	11	18	theme	fucosylated	1880:1890	arg1	N-glycopeptides					1892:1906	human milk and mucosal fucosylated N-glycopeptides	1857:1906	N-glycopeptides	1892:1906	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	4	19	theme	pepV	913:916	arg1	genes					986:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	4	20	theme	major	741:745	arg1	permease					777:784	a major facilitator superfamily (MFS) permease	739:784	a major facilitator superfamily (MFS) permease	739:784	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	3	21	theme	core-fucosylated	631:646	arg1	structures					648:657	the core-fucosylated structures	627:657	the core-fucosylated structures of mammalian N-glycoproteins	627:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	1	22	theme	human	163:167	arg1	gut					169:171	the human gut	159:171	the human gut	159:171	The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules.					
31937642	14	23	dep	casei	2410:2414	arg1	pathway					2434:2440	a novel metabolic pathway	2416:2440	the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2374:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	0	24	theme	N-Glycan	54:61	arg1	Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine					75:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	11	25	theme	gastrointestinal	1915:1930	arg1	tract.IMPORTANCE					1932:1947	the gastrointestinal tract.IMPORTANCE	1911:1947	the gastrointestinal tract.IMPORTANCE	1911:1947	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	13	26	theme	available	2244:2252	arg1	resources					2234:2242	host-derived carbon and energy resources	2203:2242	host-derived carbon and energy resources available at the mucosal surfaces	2203:2276	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	4	27	theme	alfHC	723:727	arg1	genes					717:721	the genes	713:721	the genes alfHC	713:727	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	5	28	theme	efficient	1074:1082	arg1	utilization					1093:1103	efficient 6'FN-Asn utilization	1074:1103	efficient 6'FN-Asn utilization	1074:1103	Knockout mutants showed that alfH, alfC, asdA, asnA2, and sugK are necessary for efficient 6'FN-Asn utilization.					
31937642	2	29	theme	N-glycoproteins	282:296	arg1	moiety					272:277	the glycosidic moiety	257:277	the glycosidic moiety of N-glycoproteins	257:296	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	1	30	link	host-derived	222:233	arg1	molecules					235:243	host-derived molecules	222:243	host-derived molecules	222:243	The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules.					
31937642	8	31	theme	moiety	1437:1442	arg1	presence					1413:1420	the presence	1409:1420	the presence of a 6'-fucose moiety	1409:1442	However, GlcNAc-Asn did not support growth in this mutant background, indicating that the presence of a 6'-fucose moiety is crucial for substrate transport via AlfH.					
31937642	8	31	theme	moiety	1437:1442	arg1	crucial					1447:1453	crucial	1447:1453	crucial	1447:1453	However, GlcNAc-Asn did not support growth in this mutant background, indicating that the presence of a 6'-fucose moiety is crucial for substrate transport via AlfH.					
31937642	11	32	theme	likely	1838:1843	arg1	pathway					1830:1836	an exclusive metabolic pathway	1807:1836	an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1807:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	4	33	theme	superfamily	759:769	arg1	permease					777:784	a major facilitator superfamily (MFS) permease	739:784	a major facilitator superfamily (MFS) permease	739:784	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	11	34	theme	metabolic	1820:1828	arg1	pathway					1830:1836	an exclusive metabolic pathway	1807:1836	an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1807:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	0	35	theme	Unique	0:5	arg1	Pathway					27:33	Unique Microbial Catabolic Pathway	0:33	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	0:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	13	36	theme	host-derived	2203:2214	arg1	resources					2234:2242	host-derived carbon and energy resources	2203:2242	host-derived carbon and energy resources available at the mucosal surfaces	2203:2276	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	15	37	theme	dysbiotic	2800:2808	arg1	microbiota					2810:2819	dysbiotic microbiota	2800:2819	dysbiotic microbiota	2800:2819	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	3	38	theme	N-glycoproteins	672:686	arg1	structures					648:657	the core-fucosylated structures	627:657	the core-fucosylated structures of mammalian N-glycoproteins	627:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	8	39	theme	substrate	1459:1467	arg1	transport					1469:1477	substrate transport	1459:1477	substrate transport via AlfH	1459:1486	However, GlcNAc-Asn did not support growth in this mutant background, indicating that the presence of a 6'-fucose moiety is crucial for substrate transport via AlfH.					
31937642	4	40	theme	MFS	772:774	arg1	permease					777:784	a major facilitator superfamily (MFS) permease	739:784	a major facilitator superfamily (MFS) permease	739:784	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	3	41	theme	strain	486:491	arg1	BL23					493:496	strain BL23 a gene cluster (alf-2)	486:519	Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins	466:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	0	42	theme	Catabolic	17:25	arg1	Pathway					27:33	Unique Microbial Catabolic Pathway	0:33	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	0:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	14	43	theme	core-fucosylated	2537:2552	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	1	44	theme	commensal	137:145	arg1	bacteria					147:154	commensal bacteria	137:154	commensal bacteria	137:154	The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules.					
31937642	7	45	theme	genes	1239:1243	arg1	expression					1219:1228	The constitutive expression	1202:1228	The constitutive expression of alf-2 genes in an alfR2 strain	1202:1262	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	11	46	theme	GlcNAc-Asn	1633:1642	arg1	hydrolysis					1644:1653	GlcNAc-Asn hydrolysis	1633:1653	GlcNAc-Asn hydrolysis	1633:1653	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	15	47	theme	gut	2745:2747	arg1	homeostasis					2760:2770	gut microbiota homeostasis	2745:2770	gut microbiota homeostasis in diseases associated with dysbiotic microbiota	2745:2819	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	5	48	theme	Knockout	993:1000	arg1	mutants					1002:1008	Knockout mutants	993:1008	Knockout mutants	993:1008	Knockout mutants showed that alfH, alfC, asdA, asnA2, and sugK are necessary for efficient 6'FN-Asn utilization.					
31937642	7	49	from	expression	1219:1228	arg1	strain					1257:1262	an alfR2 strain	1248:1262	an alfR2 strain	1248:1262	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	11	50	gly	N-glycopeptides	1892:1906	arg2	N-glycopeptides					1892:1906	human milk and mucosal fucosylated N-glycopeptides	1857:1906	N-glycopeptides	1892:1906	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	15	51	theme	new	2719:2721	arg1	strategies					2723:2732	new strategies	2719:2732	new strategies	2719:2732	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	4	52	theme	asdA	844:847	arg1	genes					986:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	14	53	theme	novel	2418:2422	arg1	pathway					2434:2440	a novel metabolic pathway	2416:2440	the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2374:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	11	54	theme	commensal	1776:1784	arg1	species					1796:1802	a commensal bacterial species	1774:1802	a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1774:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	0	55	theme	Human	43:47	arg1	Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine					75:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	11	56	used	used	1696:1699	arg2	GlcNAc					1679:1684	GlcNAc	1679:1684	GlcNAc	1679:1684	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	11	56	used	used	1696:1699	arg2	aspartate					1665:1673	aspartate	1665:1673	aspartate	1665:1673	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	11	56	used	used	1696:1699	arg2	source					1718:1723	a fermentable source	1704:1723	a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1704:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	7	57	theme	constitutive	1206:1217	arg1	expression					1219:1228	The constitutive expression	1202:1228	The constitutive expression of alf-2 genes in an alfR2 strain	1202:1262	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	2	58	theme	moiety	272:277	arg1	use					250:252	The use	246:252	The use of the glycosidic moiety of N-glycoproteins by bacteria	246:308	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	2	59	theme	N-glycopeptides	345:359	arg1	acids					375:379	N-glycopeptides or glycoamino acids	345:379	acids	375:379	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	3	60	dep	casei	480:484	arg1	BL23					493:496	strain BL23 a gene cluster (alf-2)	486:519	Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins	466:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	14	61	located	present	2522:2528	arg2	fucosyl-α-1,6-N-GlcNAc-Asn					2485:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	61	located	present	2522:2528	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	61	located	present	2522:2528	arg2	present					2522:2528	present	2522:2528	present	2522:2528	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	62	theme	species	2388:2394	arg1	casei					2410:2414	the commensal species Lactobacillus casei	2374:2414	the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2374:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	1	63	from	survival	125:132	arg1	gut					169:171	the human gut	159:171	the human gut	159:171	The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules.					
31937642	14	64	theme	acid	2480:2483	arg1	fucosyl-α-1,6-N-GlcNAc-Asn					2485:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	64	theme	acid	2480:2483	arg1	present					2522:2528	present	2522:2528	present	2522:2528	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	3	65	dep	BL23	493:496	arg1	cluster					505:511	a gene cluster	498:511	Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins	466:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	65	dep	BL23	493:496	arg1	alf-2					514:518	alf-2	514:518	alf-2	514:518	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	9	66	gly	defucosylated	1518:1530	arg1	6'FN-Asn					1506:1513	6'FN-Asn	1506:1513	6'FN-Asn	1506:1513	Within bacteria, 6'FN-Asn is defucosylated by AlfC, generating GlcNAc-Asn.					
31937642	13	67	from	advantage	2324:2332	arg1	gut					2341:2343	the gut	2337:2343	the gut	2337:2343	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	11	68	from	existence	1761:1769	arg1	species					1796:1802	a commensal bacterial species	1774:1802	a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1774:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	2	69	theme	glycoamino	364:373	arg1	acids					375:379	N-glycopeptides or glycoamino acids	345:379	acids	375:379	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	6	70	theme	AlfR2	1185:1189	arg1	regulator					1191:1199	the AlfR2 regulator	1181:1199	the AlfR2 regulator	1181:1199	The alf-2 genes are induced by 6'FN-Asn, but not by its glycan moiety, via the AlfR2 regulator.					
31937642	11	71	theme	gastrointestinal	1953:1968	arg1	tract					1970:1974	The gastrointestinal tract	1949:1974	The gastrointestinal tract	1949:1974	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	13	72	theme	competitive	2312:2322	arg1	advantage					2324:2332	a competitive advantage	2310:2332	a competitive advantage in the gut	2310:2343	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	2	73	gly	N-glycoproteins	282:296	arg1	N-glycoproteins					282:296	N-glycoproteins	282:296	N-glycoproteins	282:296	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	14	74	gly	core-fucosylated	2537:2552	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	3	75	theme	fucosyl-α-1,6-N-GlcNAc-Asn	571:596	arg1	catabolism					537:546	the catabolism	533:546	the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins	533:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	11	76	dep	L.casei	1728:1734	arg1	establish					1747:1755	establish	1747:1755	establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1747:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	12	77	theme	administered	2112:2123	arg1	probiotics					2125:2134	administered probiotics	2112:2134	administered probiotics	2112:2134	The mechanisms enabling commensal bacteria and administered probiotics to colonize the gut remain largely unknown.					
31937642	13	78	from	surfaces	2269:2276	arg1	available					2244:2252	available	2244:2252	available	2244:2252	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	3	79	theme	glycoamino	555:564	arg1	6'FN-Asn					599:606	6'FN-Asn	599:606	6'FN-Asn	599:606	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	79	theme	glycoamino	555:564	arg1	fucosyl-α-1,6-N-GlcNAc-Asn					571:596	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	551:596	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn)	551:607	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	79	theme	glycoamino	555:564	arg1	constituent					612:622	a constituent	610:622	a constituent of the core-fucosylated structures of mammalian N-glycoproteins	610:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	1	80	theme	host-derived	222:233	arg1	molecules					235:243	host-derived molecules	222:243	host-derived molecules	222:243	The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules.					
31937642	11	81	from	N-glycopeptides	1892:1906	arg1	tract.IMPORTANCE					1932:1947	the gastrointestinal tract.IMPORTANCE	1911:1947	the gastrointestinal tract.IMPORTANCE	1911:1947	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	2	82	theme	glycosidic	261:270	arg1	moiety					272:277	the glycosidic moiety	257:277	the glycosidic moiety of N-glycoproteins	257:296	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	7	83	theme	6'-fucosyl-glycans	1303:1320	arg1	variety					1292:1298	a variety	1290:1298	a variety of 6'-fucosyl-glycans	1290:1320	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	7	83	theme	6'-fucosyl-glycans	1303:1320	arg1	6'-fucosyl-glycans					1303:1320	6'-fucosyl-glycans	1303:1320	6'-fucosyl-glycans	1303:1320	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	11	84	theme	mucosal	1872:1878	arg1	N-glycopeptides					1892:1906	human milk and mucosal fucosylated N-glycopeptides	1857:1906	N-glycopeptides	1892:1906	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	1	85	theme	bacteria	147:154	arg1	survival					125:132	The survival	121:132	The survival of commensal bacteria in the human gut	121:171	The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules.					
31937642	7	86	theme	variety	1292:1298	arg1	metabolism					1276:1285	the metabolism	1272:1285	the metabolism of a variety of 6'-fucosyl-glycans	1272:1320	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	11	87	from	milk	1863:1866	arg1	tract.IMPORTANCE					1932:1947	the gastrointestinal tract.IMPORTANCE	1911:1947	the gastrointestinal tract.IMPORTANCE	1911:1947	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	11	88	theme	human	2051:2055	arg1	health					2057:2062	human health	2051:2062	human health	2051:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	14	89	gly	N-glycoproteins	2554:2568	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	0	90	theme	Constituent	63:73	arg1	Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine					75:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	14	91	attach	present	2522:2528	arg2	fucosyl-α-1,6-N-GlcNAc-Asn					2485:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	2465:2510	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	91	attach	present	2522:2528	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	14	91	attach	present	2522:2528	arg2	present					2522:2528	present	2522:2528	present	2522:2528	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	11	92	contain	have	2024:2027	arg2	impact					2041:2046	an enormous impact	2029:2046	an enormous impact on human health	2029:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	11	92	contain	have	2024:2027	arg1	microorganisms					2004:2017	more than 1014 microorganisms	1989:2017	more than 1014 microorganisms that have an enormous impact on human health	1989:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	4	93	theme	sugK	966:969	arg1	genes					986:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	8	94	theme	6'-fucose	1427:1435	arg1	moiety					1437:1442	a 6'-fucose moiety	1425:1442	a 6'-fucose moiety	1425:1442	However, GlcNAc-Asn did not support growth in this mutant background, indicating that the presence of a 6'-fucose moiety is crucial for substrate transport via AlfH.					
31937642	11	95	theme	fermentable	1706:1716	arg1	source					1718:1723	a fermentable source	1704:1723	a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1704:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	11	95	theme	fermentable	1706:1716	arg1	aspartate					1665:1673	aspartate	1665:1673	aspartate	1665:1673	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	11	95	theme	fermentable	1706:1716	arg1	GlcNAc					1679:1684	GlcNAc	1679:1684	GlcNAc	1679:1684	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	5	96	theme	6'FN-Asn	1084:1091	arg1	utilization					1093:1103	efficient 6'FN-Asn utilization	1074:1103	efficient 6'FN-Asn utilization	1074:1103	Knockout mutants showed that alfH, alfC, asdA, asnA2, and sugK are necessary for efficient 6'FN-Asn utilization.					
31937642	4	97	theme	asnA2	931:935	arg1	genes					986:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes	819:990	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	10	98	theme	glycosylasparaginase	1605:1624	arg1	AsnA2					1626:1630	the glycosylasparaginase AsnA2	1601:1630	the glycosylasparaginase AsnA2	1601:1630	This glycoamino acid is processed by the glycosylasparaginase AsnA2.					
31937642	15	99	theme	commensal/probiotic	2667:2685	arg1	bacteria					2687:2694	the host and commensal/probiotic bacteria	2654:2694	bacteria	2687:2694	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	7	100	theme	alfR2	1251:1255	arg1	strain					1257:1262	an alfR2 strain	1248:1262	an alfR2 strain	1248:1262	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	11	101	from	impact	2041:2046	arg1	health					2057:2062	human health	2051:2062	human health	2051:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	10	102	theme	glycoamino	1569:1578	arg1	acid					1580:1583	This glycoamino acid	1564:1583	This glycoamino acid	1564:1583	This glycoamino acid is processed by the glycosylasparaginase AsnA2.					
31937642	0	103	theme	Microbial	7:15	arg1	Pathway					27:33	Unique Microbial Catabolic Pathway	0:33	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	0:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	15	104	from	homeostasis	2760:2770	arg1	diseases					2775:2782	diseases	2775:2782	diseases associated with dysbiotic microbiota	2775:2819	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	4	105	theme	facilitator	747:757	arg1	permease					777:784	a major facilitator superfamily (MFS) permease	739:784	a major facilitator superfamily (MFS) permease	739:784	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	11	106	theme	pathway	1830:1836	arg1	species					1796:1802	a commensal bacterial species	1774:1802	a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1774:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	15	107	theme	microbiota	2749:2758	arg1	homeostasis					2760:2770	gut microbiota homeostasis	2745:2770	gut microbiota homeostasis in diseases associated with dysbiotic microbiota	2745:2819	These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.					
31937642	13	108	theme	carbon	2216:2221	arg1	resources					2234:2242	host-derived carbon and energy resources	2203:2242	host-derived carbon and energy resources available at the mucosal surfaces	2203:2276	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	7	109	theme	alf-2	1233:1237	arg1	genes					1239:1243	alf-2 genes	1233:1243	alf-2 genes	1233:1243	The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6'-fucosyl-glycans.					
31937642	14	110	from	present	2522:2528	arg1	N-glycoproteins					2554:2568	the core-fucosylated N-glycoproteins	2533:2568	the core-fucosylated N-glycoproteins from mammalians	2533:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	3	111	theme	mammalian	662:670	arg1	N-glycoproteins					672:686	mammalian N-glycoproteins	662:686	mammalian N-glycoproteins	662:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	13	112	theme	energy	2227:2232	arg1	resources					2234:2242	host-derived carbon and energy resources	2203:2242	host-derived carbon and energy resources available at the mucosal surfaces	2203:2276	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	4	113	theme	sugar	972:976	arg1	kinase					978:983	sugar kinase	972:983	sugar kinase	972:983	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	4	113	theme	sugar	972:976	arg1	asdA					844:847	the divergently oriented asdA	819:847	the divergently oriented asdA (aspartate 4-decarboxylase)	819:875	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	3	114	theme	structures	648:657	arg1	fucosyl-α-1,6-N-GlcNAc-Asn					571:596	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn	551:596	the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn)	551:607	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	114	theme	structures	648:657	arg1	constituent					612:622	a constituent	610:622	a constituent of the core-fucosylated structures of mammalian N-glycoproteins	610:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	14	115	theme	metabolic	2424:2432	arg1	pathway					2434:2440	a novel metabolic pathway	2416:2440	the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2374:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	11	116	theme	human	1857:1861	arg1	milk					1863:1866	human milk and mucosal fucosylated N-glycopeptides	1857:1906	milk	1863:1866	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	0	117	theme	Core	49:52	arg1	Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine					75:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine	39:118	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine.					
31937642	11	118	gly	fucosylated	1880:1890	arg1	N-glycopeptides					1892:1906	human milk and mucosal fucosylated N-glycopeptides	1857:1906	N-glycopeptides	1892:1906	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	4	119	theme	oriented	835:842	arg1	4-decarboxylase					860:874	aspartate 4-decarboxylase	850:874	aspartate 4-decarboxylase	850:874	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	4	119	theme	oriented	835:842	arg1	kinase					978:983	sugar kinase	972:983	sugar kinase	972:983	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	4	119	theme	oriented	835:842	arg1	asdA					844:847	the divergently oriented asdA	819:847	the divergently oriented asdA (aspartate 4-decarboxylase)	819:875	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	11	120	theme	bacterial	1786:1794	arg1	species					1796:1802	a commensal bacterial species	1774:1802	a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1774:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	6	121	theme	glycan	1162:1167	arg1	moiety					1169:1174	its glycan moiety	1158:1174	its glycan moiety	1158:1174	The alf-2 genes are induced by 6'FN-Asn, but not by its glycan moiety, via the AlfR2 regulator.					
31937642	14	122	theme	commensal	2378:2386	arg1	species					2388:2394	commensal species	2378:2394	the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2374:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	2	123	gly	N-glycopeptides	345:359	arg2	N-glycopeptides					345:359	N-glycopeptides or glycoamino acids	345:379	N-glycopeptides	345:359	The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated.					
31937642	4	124	theme	α-l-fucosidase	794:807	arg1	AlfC					809:812	the α-l-fucosidase AlfC	790:812	the α-l-fucosidase AlfC	790:812	The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes.					
31937642	14	125	theme	Lactobacillus	2396:2408	arg1	casei					2410:2414	the commensal species Lactobacillus casei	2374:2414	the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2374:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	3	126	theme	gene	500:503	arg1	cluster					505:511	a gene cluster	498:511	Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins	466:686	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	3	126	theme	gene	500:503	arg1	alf-2					514:518	alf-2	514:518	alf-2	514:518	We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6'FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins.					
31937642	14	127	theme	fucosyl-α-1,6-N-GlcNAc-Asn	2485:2510	arg1	utilization					2450:2460	the utilization	2446:2460	the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians	2446:2584	Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians.					
31937642	11	128	dep	milk	1863:1866	arg1	accommodates					1976:1987	accommodates	1976:1987	accommodates more than 1014 microorganisms that have an enormous impact on human health	1976:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
31937642	13	129	link	host-derived	2203:2214	arg1	resources					2234:2242	host-derived carbon and energy resources	2203:2242	host-derived carbon and energy resources available at the mucosal surfaces	2203:2276	The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut.					
31937642	11	130	theme	exclusive	1810:1818	arg1	pathway					1830:1836	an exclusive metabolic pathway	1807:1836	an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health	1807:2062	GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L.casei These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract.IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health.					
33561156	2	0	theme	acid	473:476	arg1	hydrolysis					478:487	sulfuric acid hydrolysis	464:487	sulfuric acid hydrolysis	464:487	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	12	1	theme	high	1648:1651	arg1	proportion					1653:1662	high proportion	1648:1662	high proportion of CNCs	1648:1670	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	3	2	theme	cellulose	544:552	arg1	fibers					554:559	cellulose fibers	544:559	cellulose fibers	544:559	Thus, the current study aims to isolate cellulose fibers and CNCs from KW as alternative source.					
33561156	1	3	theme	pretreatment	302:313	arg1	stage					315:319	the pretreatment stage	298:319	the pretreatment stage	298:319	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	4	theme	chlorine-free	186:198	arg1	process					200:206	chlorine-free process	186:206	chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage	186:319	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	12	5	dep	/cm2	1714:1717	arg1	%					1713:1713	%	1713:1713	%	1713:1713	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	13	6	theme	cellulose	1756:1764	arg1	fibers					1766:1771	cellulose fibers	1756:1771	cellulose fibers	1756:1771	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	2	7	attach	isolated	433:440	arg2	CNCs80					415:420	CNCs80	415:420	CNCs80	415:420	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	7	attach	isolated	433:440	arg1	C80					455:457	C80	455:457	C80	455:457	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	7	attach	isolated	433:440	arg2	CNCs40					404:409	CNCs40	404:409	CNCs40	404:409	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	7	attach	isolated	433:440	arg1	C40					447:449	C40	447:449	C40	447:449	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	7	attach	isolated	433:440	arg2	nanocrystals					390:401	Cellulose nanocrystals	380:401	Cellulose nanocrystals (CNCs40 and CNCs80)	380:421	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	6	8	theme	cellulose	999:1007	arg1	fibers					1009:1014	the cellulose fibers	995:1014	the cellulose fibers	995:1014	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	4	9	theme	cellulose	609:617	arg1	fibers					619:624	cellulose fibers	609:624	cellulose fibers	609:624	The KW, cellulose fibers, and CNCs were investigated for yield, chemical composition, functionality, crystallinity, morphology, and thermal stability.					
33561156	1	10	theme	khat	150:153	arg1	KW					177:178	KW	177:178	KW	177:178	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	10	theme	khat	150:153	arg1	waste					170:174	khat (Catha edulis) waste	150:174	khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage	150:319	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	11	theme	%	275:275	arg1	acid					284:287	formic acid/80% acetic acid	261:287	80% formic acid/80% acetic acid (C80)	257:293	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	11	theme	%	275:275	arg1	C80					290:292	C80	290:292	C80	290:292	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	12	theme	Catha	156:160	arg1	KW					177:178	KW	177:178	KW	177:178	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	12	theme	Catha	156:160	arg1	waste					170:174	khat (Catha edulis) waste	150:174	khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage	150:319	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	12	13	dep	%	1713:1713	arg1	50					1711:1712	50	1711:1712	50	1711:1712	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	4	14	theme	thermal	733:739	arg1	stability					741:749	thermal stability	733:749	thermal stability	733:749	The KW, cellulose fibers, and CNCs were investigated for yield, chemical composition, functionality, crystallinity, morphology, and thermal stability.					
33561156	1	15	theme	Cellulose	99:107	arg1	fibers					109:114	Cellulose fibers	99:114	Cellulose fibers (C40 and C80)	99:128	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	15	theme	Cellulose	99:107	arg1	C80					125:127	C80	125:127	C80	125:127	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	15	theme	Cellulose	99:107	arg1	C40					117:119	C40	117:119	C40	117:119	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	8	16	theme	cellulose	1167:1175	arg1	fibers					1177:1182	The cellulose fibers	1163:1182	The cellulose fibers	1163:1182	The cellulose fibers and CNCs exhibited Cellulose Iβ crystalline lattice.					
33561156	11	17	theme	colloidal	1587:1595	arg1	stability					1609:1617	colloidal and thermal stability	1587:1617	stability	1609:1617	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	2	18	theme	Cellulose	380:388	arg1	CNCs80					415:420	CNCs80	415:420	CNCs80	415:420	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	18	theme	Cellulose	380:388	arg1	CNCs40					404:409	CNCs40	404:409	CNCs40	404:409	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	18	theme	Cellulose	380:388	arg1	nanocrystals					390:401	Cellulose nanocrystals	380:401	Cellulose nanocrystals (CNCs40 and CNCs80)	380:421	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	5	19	from	size	811:814	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	1	20	dep	%	216:216	arg1	C40					247:249	C40	247:249	C40	247:249	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	20	dep	%	216:216	arg1	acid					241:244	formic acid/40% acetic acid	218:244	40% formic acid/40% acetic acid (C40)	214:250	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	8	21	theme	Iβ	1213:1214	arg1	lattice					1228:1234	Cellulose Iβ crystalline lattice	1203:1234	Cellulose Iβ crystalline lattice	1203:1234	The cellulose fibers and CNCs exhibited Cellulose Iβ crystalline lattice.					
33561156	11	22	theme	thermal	1601:1607	arg1	stability					1609:1617	colloidal and thermal stability	1587:1617	stability	1609:1617	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	0	23	theme	cellulose	64:72	arg1	fibers					74:79	cellulose fibers	64:79	cellulose fibers	64:79	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	13	24	theme	value-added	1864:1874	arg1	applications					1887:1898	value-added industrial applications	1864:1898	value-added industrial applications	1864:1898	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	11	25	from	CNCs80	1550:1555	arg1	terms					1560:1564	terms	1560:1564	terms of aspect ratio, and colloidal and thermal stability	1560:1617	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	6	26	from	fibers	1009:1014	arg1	removal					949:955	the removal	945:955	the removal of most hemicellulose and lignin from the cellulose fibers	945:1014	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	1	27	theme	formic	261:266	arg1	acid					284:287	formic acid/80% acetic acid	261:287	80% formic acid/80% acetic acid (C80)	257:293	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	27	theme	formic	261:266	arg1	C80					290:292	C80	290:292	C80	290:292	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	6	28	theme	FTIR	916:919	arg1	analysis					929:936	The FTIR spectra analysis	912:936	The FTIR spectra analysis	912:936	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	5	29	from	formulations	884:895	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	3	30	theme	alternative	581:591	arg1	source					593:598	alternative source	581:598	alternative source	581:598	Thus, the current study aims to isolate cellulose fibers and CNCs from KW as alternative source.					
33561156	7	31	theme	XRD	1021:1023	arg1	results					1025:1031	The XRD results	1017:1031	The XRD results	1017:1031	The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs.					
33561156	5	32	theme	colloidal	781:789	arg1	stability					791:799	colloidal stability	781:799	colloidal stability	781:799	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	0	33	theme	khat	16:19	arg1	waste					36:40	khat (Catha edulis) waste	16:40	khat (Catha edulis) waste	16:40	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	1	34	theme	formic	218:223	arg1	C40					247:249	C40	247:249	C40	247:249	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	34	theme	formic	218:223	arg1	acid					241:244	formic acid/40% acetic acid	218:244	40% formic acid/40% acetic acid (C40)	214:250	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	3	35	theme	current	514:520	arg1	study					522:526	the current study	510:526	the current study	510:526	Thus, the current study aims to isolate cellulose fibers and CNCs from KW as alternative source.					
33561156	0	36	theme	Catha	22:26	arg1	waste					36:40	khat (Catha edulis) waste	16:40	khat (Catha edulis) waste	16:40	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	1	37	theme	%	232:232	arg1	C40					247:249	C40	247:249	C40	247:249	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	37	theme	%	232:232	arg1	acid					241:244	formic acid/40% acetic acid	218:244	40% formic acid/40% acetic acid (C40)	214:250	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	5	38	theme	diclofenac	849:858	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	12	39	theme	CNCs	1667:1670	arg1	proportion					1653:1662	high proportion	1648:1662	high proportion of CNCs	1648:1670	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	4	40	theme	chemical	665:672	arg1	composition					674:684	chemical composition	665:684	chemical composition	665:684	The KW, cellulose fibers, and CNCs were investigated for yield, chemical composition, functionality, crystallinity, morphology, and thermal stability.					
33561156	12	41	theme	sodium	1693:1698	arg1	/cm2					1714:1717	< 50%/cm2	1709:1717	< 50%/cm2	1709:1717	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	12	41	theme	sodium	1693:1698	arg1	release					1700:1706	diclofenac sodium release	1682:1706	diclofenac sodium release (< 50%/cm2)	1682:1718	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	12	42	theme	diclofenac	1682:1691	arg1	/cm2					1714:1717	< 50%/cm2	1709:1717	< 50%/cm2	1709:1717	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	12	42	theme	diclofenac	1682:1691	arg1	release					1700:1706	diclofenac sodium release	1682:1706	diclofenac sodium release (< 50%/cm2)	1682:1718	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	6	43	theme	hemicellulose	965:977	arg1	removal					949:955	the removal	945:955	the removal of most hemicellulose and lignin from the cellulose fibers	945:1014	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	1	44	dep	%	259:259	arg1	acid					284:287	formic acid/80% acetic acid	261:287	80% formic acid/80% acetic acid (C80)	257:293	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	44	dep	%	259:259	arg1	C80					290:292	C80	290:292	C80	290:292	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	10	45	theme	polydispersity	1379:1392	arg1	index					1394:1398	polydispersity index	1379:1398	polydispersity index	1379:1398	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	11	46	theme	ratio	1576:1580	arg1	terms					1560:1564	terms	1560:1564	terms of aspect ratio, and colloidal and thermal stability	1560:1617	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	7	47	theme	cellulose	1136:1144	arg1	fibers					1146:1151	cellulose fibers	1136:1151	cellulose fibers	1136:1151	The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs.					
33561156	13	48	theme	abundant	1824:1831	arg1	KW					1857:1858	KW	1857:1858	KW	1857:1858	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	13	48	theme	abundant	1824:1831	arg1	source					1849:1854	abundant and unexploited source	1824:1854	abundant and unexploited source	1824:1854	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	9	49	theme	needle-shaped	1272:1284	arg1	rods					1296:1299	needle-shaped nanoscale rods	1272:1299	needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73)	1272:1353	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	5	50	theme	in	840:841	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	1	51	with	waste	170:174	arg1	process					200:206	chlorine-free process	186:206	chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage	186:319	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	52	theme	acetic	234:239	arg1	C40					247:249	C40	247:249	C40	247:249	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	52	theme	acetic	234:239	arg1	acid					241:244	formic acid/40% acetic acid	218:244	40% formic acid/40% acetic acid (C40)	214:250	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	10	53	theme	zeta	1405:1408	arg1	potential					1410:1418	zeta potential	1405:1418	zeta potential	1405:1418	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	13	54	theme	unexploited	1837:1847	arg1	KW					1857:1858	KW	1857:1858	KW	1857:1858	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	13	54	theme	unexploited	1837:1847	arg1	source					1849:1854	abundant and unexploited source	1824:1854	abundant and unexploited source	1824:1854	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	9	55	theme	rods	1296:1299	arg1	formation					1259:1267	formation	1259:1267	formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73)	1259:1353	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	2	56	theme	sulfuric	464:471	arg1	hydrolysis					478:487	sulfuric acid hydrolysis	464:487	sulfuric acid hydrolysis	464:487	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	2	57	with	C40	447:449	arg1	hydrolysis					478:487	sulfuric acid hydrolysis	464:487	sulfuric acid hydrolysis	464:487	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	12	58	theme	Gel	1620:1622	arg1	formulations					1624:1635	Gel formulations	1620:1635	Gel formulations containing high proportion of CNCs	1620:1670	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	10	59	theme	CNCS	1427:1430	arg1	potential					1410:1418	zeta potential	1405:1418	zeta potential	1405:1418	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	10	59	theme	CNCS	1427:1430	arg1	size					1373:1376	The hydrodynamic size	1356:1376	The hydrodynamic size	1356:1376	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	10	59	theme	CNCS	1427:1430	arg1	index					1394:1398	polydispersity index	1379:1398	polydispersity index	1379:1398	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	11	60	theme	superior	1527:1534	arg1	properties					1536:1545	superior properties	1527:1545	superior properties	1527:1545	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	2	61	with	C80	455:457	arg1	hydrolysis					478:487	sulfuric acid hydrolysis	464:487	sulfuric acid hydrolysis	464:487	Cellulose nanocrystals (CNCs40 and CNCs80) were then isolated from C40 and C80 with sulfuric acid hydrolysis, respectively.					
33561156	1	62	theme	further	334:340	arg1	delignification					342:356	further delignification	334:356	further delignification	334:356	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	5	63	from	influence	827:835	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	6	64	theme	lignin	983:988	arg1	removal					949:955	the removal	945:955	the removal of most hemicellulose and lignin from the cellulose fibers	945:1014	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	5	65	dep	in	840:841	arg1	vitro					843:847	vitro	843:847	vitro	843:847	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	12	66	contain	containing	1637:1646	arg1	formulations					1624:1635	Gel formulations	1620:1635	Gel formulations containing high proportion of CNCs	1620:1670	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	12	66	contain	containing	1637:1646	arg2	proportion					1653:1662	high proportion	1648:1662	high proportion of CNCs	1648:1670	Gel formulations containing high proportion of CNCs sustained diclofenac sodium release (< 50%/cm2) over 12 h.					
33561156	1	67	theme	acetic	277:282	arg1	acid					284:287	formic acid/80% acetic acid	261:287	80% formic acid/80% acetic acid (C80)	257:293	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	67	theme	acetic	277:282	arg1	C80					290:292	C80	290:292	C80	290:292	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	9	68	theme	aspect	1328:1333	arg1	ratio					1335:1339	aspect ratio	1328:1339	length: 101.55-162.96 nm; aspect ratio: 12.84-22.73	1302:1352	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	1	69	theme	edulis	162:167	arg1	KW					177:178	KW	177:178	KW	177:178	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	69	theme	edulis	162:167	arg1	waste					170:174	khat (Catha edulis) waste	150:174	khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage	150:319	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	9	70	dep	length	1302:1307	arg1	12.84-22.73					1342:1352	12.84-22.73	1342:1352	length: 101.55-162.96 nm; aspect ratio: 12.84-22.73	1302:1352	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	9	70	dep	length	1302:1307	arg1	nm					1324:1325	101.55-162.96 nm	1310:1325	length: 101.55-162.96 nm; aspect ratio: 12.84-22.73	1302:1352	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	9	70	dep	length	1302:1307	arg1	ratio					1335:1339	aspect ratio	1328:1339	length: 101.55-162.96 nm; aspect ratio: 12.84-22.73	1302:1352	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	5	71	theme	gel	880:882	arg1	formulations					884:895	gel formulations	880:895	gel formulations preliminarily	880:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	9	72	dep	rods	1296:1299	arg1	length					1302:1307	length	1302:1307	length: 101.55-162.96 nm; aspect ratio: 12.84-22.73	1302:1352	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	1	73	dep	fibers	109:114	arg1	fibers					109:114	Cellulose fibers	99:114	Cellulose fibers (C40 and C80)	99:128	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	73	dep	fibers	109:114	arg1	C80					125:127	C80	125:127	C80	125:127	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	1	73	dep	fibers	109:114	arg1	C40					117:119	C40	117:119	C40	117:119	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	7	74	theme	acid	1075:1078	arg1	hydrolysis					1080:1089	acid hydrolysis	1075:1089	acid hydrolysis	1075:1089	The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs.					
33561156	7	75	theme	chemical	1048:1055	arg1	pretreatments					1057:1069	chemical pretreatments	1048:1069	chemical pretreatments	1048:1069	The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs.					
33561156	0	76	theme	fibers	74:79	arg1	production					50:59	the production	46:59	the production of cellulose fibers and nanocrystals	46:96	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	5	77	theme	particle	802:809	arg1	size					811:814	particle size	802:814	particle size	802:814	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	8	78	theme	Cellulose	1203:1211	arg1	lattice					1228:1234	Cellulose Iβ crystalline lattice	1203:1234	Cellulose Iβ crystalline lattice	1203:1234	The cellulose fibers and CNCs exhibited Cellulose Iβ crystalline lattice.					
33561156	11	79	theme	stability	1609:1617	arg1	terms					1560:1564	terms	1560:1564	terms of aspect ratio, and colloidal and thermal stability	1560:1617	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	0	80	theme	nanocrystals	85:96	arg1	production					50:59	the production	46:59	the production of cellulose fibers and nanocrystals	46:96	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	13	81	theme	industrial	1876:1885	arg1	applications					1887:1898	value-added industrial applications	1864:1898	value-added industrial applications	1864:1898	This study suggests that cellulose fibers and nanocrystals can be successfully obtained from abundant and unexploited source, KW for value-added industrial applications.					
33561156	6	82	theme	spectra	921:927	arg1	analysis					929:936	The FTIR spectra analysis	912:936	The FTIR spectra analysis	912:936	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	8	83	theme	crystalline	1216:1226	arg1	lattice					1228:1234	Cellulose Iβ crystalline lattice	1203:1234	Cellulose Iβ crystalline lattice	1203:1234	The cellulose fibers and CNCs exhibited Cellulose Iβ crystalline lattice.					
33561156	1	84	theme	acid/80	268:274	arg1	%					275:275	%	275:275	%	275:275	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	10	85	theme	hydrodynamic	1360:1371	arg1	size					1373:1376	The hydrodynamic size	1356:1376	The hydrodynamic size	1356:1376	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	0	86	theme	edulis	28:33	arg1	waste					36:40	khat (Catha edulis) waste	16:40	khat (Catha edulis) waste	16:40	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	1	87	theme	acid/40	225:231	arg1	%					232:232	%	232:232	%	232:232	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	7	88	theme	CNCs	1157:1160	arg1	crystallinity					1119:1131	the crystallinity	1115:1131	the crystallinity of cellulose fibers and CNCs	1115:1160	The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs.					
33561156	1	89	theme	bleaching	362:370	arg1	stages					372:377	bleaching stages	362:377	bleaching stages	362:377	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	0	90	theme	waste	36:40	arg1	Valorization					0:11	Valorization	0:11	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.	0:97	Valorization of khat (Catha edulis) waste for the production of cellulose fibers and nanocrystals.					
33561156	9	91	theme	TEM	1237:1239	arg1	analysis					1241:1248	TEM analysis	1237:1248	TEM analysis	1237:1248	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
33561156	5	92	theme	sodium	860:865	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	7	93	theme	fibers	1146:1151	arg1	crystallinity					1119:1131	the crystallinity	1115:1131	the crystallinity of cellulose fibers and CNCs	1115:1160	The XRD results indicated that chemical pretreatments and acid hydrolysis significantly increased the crystallinity of cellulose fibers and CNCs.					
33561156	11	94	theme	aspect	1569:1574	arg1	ratio					1576:1580	aspect ratio	1569:1580	aspect ratio	1569:1580	CNCs40 exhibited superior properties to CNCs80 in terms of aspect ratio, and colloidal and thermal stability.					
33561156	1	95	dep	extracted	135:143	arg1	followed					322:329	followed	322:329	followed by further delignification and bleaching stages	322:377	Cellulose fibers (C40 and C80) were extracted from khat (Catha edulis) waste (KW) with chlorine-free process using 40% formic acid/40% acetic acid (C40), and 80% formic acid/80% acetic acid (C80) at the pretreatment stage, followed by further delignification and bleaching stages.					
33561156	5	96	from	stability	791:799	arg1	release					867:873	in vitro diclofenac sodium release	840:873	in vitro diclofenac sodium release from gel formulations preliminarily	840:909	CNCs were also evaluated for colloidal stability, particle size, and their influence on in vitro diclofenac sodium release from gel formulations preliminarily.					
33561156	6	97	theme	most	960:963	arg1	hemicellulose					965:977	hemicellulose	965:977	hemicellulose	965:977	The FTIR spectra analysis showed the removal of most hemicellulose and lignin from the cellulose fibers.					
33561156	10	98	dep	-75.3	1486:1490	arg1	to					1483:1484	to	1483:1484	to	1483:1484	The hydrodynamic size, polydispersity index, and zeta potential of the CNCS ranged from 222.8-362.8 nm; 0.297-0.461, and -45.7 to -75.3 mV, respectively.					
33561156	9	99	theme	nanoscale	1286:1294	arg1	rods					1296:1299	needle-shaped nanoscale rods	1272:1299	needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73)	1272:1353	TEM analysis revealed formation of needle-shaped nanoscale rods (length: 101.55-162.96 nm; aspect ratio: 12.84-22.73).					
32447020	6	0	theme	nanoparticles	1094:1106	arg1	feature					1079:1085	another distinguishing feature	1056:1085	another distinguishing feature	1056:1085	Easy cyclic adsorption-desorption was another distinguishing feature of the nanoparticles, whose removal efficiency to these pollutants hardly varied after 10 cycles of regeneration.					
32447020	4	1	theme	superfast	658:666	arg1	kinetics					679:686	superfast adsorption kinetics	658:686	superfast adsorption kinetics	658:686	The resulting nanoparticles exhibited superfast adsorption kinetics to pollutants with removal efficiency of over 95% within 10 s.					
32447020	3	2	theme	antibacterial	459:471	arg1	nanoparticles					494:506	antibacterial β-cyclodextrin-based nanoparticles	459:506	antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN)	459:516	In this work, antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN) were prepared via one-pot method to explore their adsorption performance to pollutants in wastewater.					
32447020	3	2	theme	antibacterial	459:471	arg1	E-β-CDN					509:515	E-β-CDN	509:515	E-β-CDN	509:515	In this work, antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN) were prepared via one-pot method to explore their adsorption performance to pollutants in wastewater.					
32447020	2	3	with	materials	326:334	arg1	groups					360:365	complex functional groups	341:365	complex functional groups	341:365	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	7	4	theme	inhibitive	1304:1313	arg1	rate					1315:1318	99.99% bacterial inhibitive rate	1287:1318	99.99% bacterial inhibitive rate	1287:1318	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	7	5	dep	coli	1368:1371	arg1	aureus					1425:1430	Staphylococcus aureus	1410:1430	Staphylococcus aureus	1410:1430	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	7	5	dep	coli	1368:1371	arg1	aureus					1436:1441	S. aureus	1433:1441	S. aureus	1433:1441	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	7	5	dep	coli	1368:1371	arg1	coli					1377:1380	E. coli	1374:1380	E. coli	1374:1380	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	2	6	theme	micro-porous	313:324	arg1	materials					326:334	many micro-porous materials	308:334	many micro-porous materials with complex functional groups	308:365	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	6	7	theme	distinguishing	1064:1077	arg1	feature					1079:1085	another distinguishing feature	1056:1085	another distinguishing feature	1056:1085	Easy cyclic adsorption-desorption was another distinguishing feature of the nanoparticles, whose removal efficiency to these pollutants hardly varied after 10 cycles of regeneration.					
32447020	5	8	theme	many	995:998	arg1	adsorbents					1006:1015	many other adsorbents	995:1015	many other adsorbents	995:1015	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	2	9	theme	many	308:311	arg1	materials					326:334	many micro-porous materials	308:334	many micro-porous materials with complex functional groups	308:365	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	4	10	theme	adsorption	668:677	arg1	kinetics					679:686	superfast adsorption kinetics	658:686	superfast adsorption kinetics	658:686	The resulting nanoparticles exhibited superfast adsorption kinetics to pollutants with removal efficiency of over 95% within 10 s.					
32447020	5	11	theme	heavy	836:840	arg1	ions					848:851	heavy metal ions	836:851	heavy metal ions	836:851	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	5	12	dep	higher	975:980	arg1	3289.6 mg g-1					896:908	3289.6 mg g-1	896:908	3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1	896:952	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	2	13	theme	adsorption	420:429	arg1	capabilities					431:442	their high pollutant adsorption capabilities	399:442	their high pollutant adsorption capabilities	399:442	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	1	14	theme	low	196:198	arg1	processes					217:225	low energy efficient processes	196:225	low energy efficient processes due to the complex composition of wastewater	196:270	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	5	15	theme	metal	842:846	arg1	ions					848:851	heavy metal ions	836:851	heavy metal ions	836:851	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	5	16	theme	maximum	864:870	arg1	capacity					883:890	their maximum adsorption capacity	858:890	their maximum adsorption capacity	858:890	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	7	17	theme	99.99	1287:1291	arg1	%					1292:1292	%	1292:1292	%	1292:1292	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	1	18	theme	energy	200:205	arg1	processes					217:225	low energy efficient processes	196:225	low energy efficient processes due to the complex composition of wastewater	196:270	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	6	19	theme	Easy	1018:1021	arg1	adsorption-desorption					1030:1050	Easy cyclic adsorption-desorption	1018:1050	Easy cyclic adsorption-desorption	1018:1050	Easy cyclic adsorption-desorption was another distinguishing feature of the nanoparticles, whose removal efficiency to these pollutants hardly varied after 10 cycles of regeneration.					
32447020	2	20	theme	functional	349:358	arg1	groups					360:365	complex functional groups	341:365	complex functional groups	341:365	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	1	21	theme	efficient	207:215	arg1	processes					217:225	low energy efficient processes	196:225	low energy efficient processes due to the complex composition of wastewater	196:270	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	0	22	theme	One-pot	0:6	arg1	fabrication					8:18	One-pot fabrication	0:18	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles	0:70	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	7	23	theme	prominent	1251:1259	arg1	activity					1275:1282	prominent antibacterial activity	1251:1282	prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus)	1251:1442	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	2	24	theme	complex	341:347	arg1	groups					360:365	complex functional groups	341:365	complex functional groups	341:365	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	7	25	dep	bacteria	1347:1354	arg1	bacteria					1401:1408	gram-positive bacteria	1387:1408	gram-positive bacteria	1387:1408	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	7	25	dep	bacteria	1347:1354	arg1	coli					1368:1371	Escherichia coli	1356:1371	Escherichia coli (E. coli)	1356:1381	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	2	26	theme	pollutant	410:418	arg1	capabilities					431:442	their high pollutant adsorption capabilities	399:442	their high pollutant adsorption capabilities	399:442	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	0	27	theme	antibacterial	23:35	arg1	nanoparticles					58:70	antibacterial β-cyclodextrin-based nanoparticles	23:70	antibacterial β-cyclodextrin-based nanoparticles	23:70	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	5	28	theme	broad-spectrum	784:797	arg1	adsorption					799:808	broad-spectrum adsorption	784:808	broad-spectrum adsorption to organic pollutants	784:830	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	1	29	theme	due	227:229	arg1	processes					217:225	low energy efficient processes	196:225	low energy efficient processes due to the complex composition of wastewater	196:270	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	5	30	theme	other	1000:1004	arg1	adsorbents					1006:1015	many other adsorbents	995:1015	many other adsorbents	995:1015	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	6	31	theme	cyclic	1023:1028	arg1	adsorption-desorption					1030:1050	Easy cyclic adsorption-desorption	1018:1050	Easy cyclic adsorption-desorption	1018:1050	Easy cyclic adsorption-desorption was another distinguishing feature of the nanoparticles, whose removal efficiency to these pollutants hardly varied after 10 cycles of regeneration.					
32447020	0	32	dep	superfast	82:90	arg1	broad-spectrum					93:106	broad-spectrum	93:106	broad-spectrum	93:106	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	3	33	theme	adsorption	568:577	arg1	performance					579:589	their adsorption performance	562:589	their adsorption performance	562:589	In this work, antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN) were prepared via one-pot method to explore their adsorption performance to pollutants in wastewater.					
32447020	0	34	theme	nanoparticles	58:70	arg1	adsorption					108:117	their superfast, broad-spectrum adsorption	76:117	their superfast, broad-spectrum adsorption towards pollutants	76:136	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	0	34	theme	nanoparticles	58:70	arg1	fabrication					8:18	One-pot fabrication	0:18	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles	0:70	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	4	35	theme	resulting	624:632	arg1	nanoparticles					634:646	The resulting nanoparticles	620:646	The resulting nanoparticles	620:646	The resulting nanoparticles exhibited superfast adsorption kinetics to pollutants with removal efficiency of over 95% within 10 s.					
32447020	3	36	theme	β-cyclodextrin-based	473:492	arg1	nanoparticles					494:506	antibacterial β-cyclodextrin-based nanoparticles	459:506	antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN)	459:516	In this work, antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN) were prepared via one-pot method to explore their adsorption performance to pollutants in wastewater.					
32447020	3	36	theme	β-cyclodextrin-based	473:492	arg1	E-β-CDN					509:515	E-β-CDN	509:515	E-β-CDN	509:515	In this work, antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN) were prepared via one-pot method to explore their adsorption performance to pollutants in wastewater.					
32447020	0	37	theme	β-cyclodextrin-based	37:56	arg1	nanoparticles					58:70	antibacterial β-cyclodextrin-based nanoparticles	23:70	antibacterial β-cyclodextrin-based nanoparticles	23:70	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	5	38	theme	adsorption	872:881	arg1	capacity					883:890	their maximum adsorption capacity	858:890	their maximum adsorption capacity	858:890	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	4	39	theme	removal	707:713	arg1	efficiency					715:724	removal efficiency	707:724	removal efficiency of over 95% within 10 s	707:748	The resulting nanoparticles exhibited superfast adsorption kinetics to pollutants with removal efficiency of over 95% within 10 s.					
32447020	1	40	theme	complex	238:244	arg1	composition					246:256	the complex composition	234:256	the complex composition of wastewater	234:270	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	7	41	theme	resulting	1220:1228	arg1	nanoparticles					1230:1242	the resulting nanoparticles	1216:1242	the resulting nanoparticles	1216:1242	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	8	42	theme	resulting	1476:1484	arg1	nanoparticles					1486:1498	the resulting nanoparticles	1472:1498	the resulting nanoparticles	1472:1498	These results suggest that the resulting nanoparticles have great potential in the purification of the wastewater.					
32447020	8	43	contain	have	1500:1503	arg1	nanoparticles					1486:1498	the resulting nanoparticles	1472:1498	the resulting nanoparticles	1472:1498	These results suggest that the resulting nanoparticles have great potential in the purification of the wastewater.					
32447020	8	43	contain	have	1500:1503	arg2	potential					1511:1519	great potential	1505:1519	great potential	1505:1519	These results suggest that the resulting nanoparticles have great potential in the purification of the wastewater.					
32447020	4	44	with	pollutants	691:700	arg1	efficiency					715:724	removal efficiency	707:724	removal efficiency of over 95% within 10 s	707:748	The resulting nanoparticles exhibited superfast adsorption kinetics to pollutants with removal efficiency of over 95% within 10 s.					
32447020	6	45	theme	regeneration	1187:1198	arg1	cycles					1177:1182	10 cycles	1174:1182	10 cycles of regeneration	1174:1198	Easy cyclic adsorption-desorption was another distinguishing feature of the nanoparticles, whose removal efficiency to these pollutants hardly varied after 10 cycles of regeneration.					
32447020	1	46	theme	current	143:149	arg1	technology					167:176	The current water treatment technology	139:176	The current water treatment technology	139:176	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	5	47	theme	organic	813:819	arg1	pollutants					821:830	organic pollutants	813:830	organic pollutants	813:830	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	7	48	theme	bacterial	1294:1302	arg1	rate					1315:1318	99.99% bacterial inhibitive rate	1287:1318	99.99% bacterial inhibitive rate	1287:1318	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	5	49	theme	methyl	918:923	arg1	MO					933:934	MO	933:934	MO	933:934	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	5	49	theme	methyl	918:923	arg1	orange					925:930	methyl orange	918:930	methyl orange (MO)	918:935	The nanoparticles also presented broad-spectrum adsorption to organic pollutants and heavy metal ions, and their maximum adsorption capacity was 3289.6 mg g-1 towards methyl orange (MO) and 970.8 mg g-1 towards Pb(II), much higher than that of many other adsorbents.					
32447020	1	50	theme	water	151:155	arg1	technology					167:176	The current water treatment technology	139:176	The current water treatment technology	139:176	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	7	51	theme	antibacterial	1261:1273	arg1	activity					1275:1282	prominent antibacterial activity	1251:1282	prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus)	1251:1442	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	2	52	theme	energy	289:294	arg1	efficiency					296:305	high energy efficiency	284:305	high energy efficiency	284:305	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	8	53	theme	great	1505:1509	arg1	potential					1511:1519	great potential	1505:1519	great potential	1505:1519	These results suggest that the resulting nanoparticles have great potential in the purification of the wastewater.					
32447020	1	54	theme	treatment	157:165	arg1	technology					167:176	The current water treatment technology	139:176	The current water treatment technology	139:176	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	2	55	theme	high	284:287	arg1	efficiency					296:305	high energy efficiency	284:305	high energy efficiency	284:305	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	3	56	theme	one-pot	536:542	arg1	method					544:549	one-pot method	536:549	one-pot method	536:549	In this work, antibacterial β-cyclodextrin-based nanoparticles (E-β-CDN) were prepared via one-pot method to explore their adsorption performance to pollutants in wastewater.					
32447020	7	57	theme	gram-negative	1333:1345	arg1	bacteria					1347:1354	both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus)	1328:1442	both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus)	1328:1442	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	6	58	theme	removal	1115:1121	arg1	efficiency					1123:1132	efficiency	1123:1132	efficiency	1123:1132	Easy cyclic adsorption-desorption was another distinguishing feature of the nanoparticles, whose removal efficiency to these pollutants hardly varied after 10 cycles of regeneration.					
32447020	8	59	theme	wastewater	1548:1557	arg1	purification					1528:1539	the purification	1524:1539	the purification of the wastewater	1524:1557	These results suggest that the resulting nanoparticles have great potential in the purification of the wastewater.					
32447020	2	60	theme	high	405:408	arg1	capabilities					431:442	their high pollutant adsorption capabilities	399:442	their high pollutant adsorption capabilities	399:442	To achieve high energy efficiency, many micro-porous materials with complex functional groups have been fabricated because of their high pollutant adsorption capabilities.					
32447020	4	61	theme	%	736:736	arg1	efficiency					715:724	removal efficiency	707:724	removal efficiency of over 95% within 10 s	707:748	The resulting nanoparticles exhibited superfast adsorption kinetics to pollutants with removal efficiency of over 95% within 10 s.					
32447020	1	62	theme	wastewater	261:270	arg1	composition					246:256	the complex composition	234:256	the complex composition of wastewater	234:270	The current water treatment technology is still based on low energy efficient processes due to the complex composition of wastewater.					
32447020	0	63	theme	superfast	82:90	arg1	adsorption					108:117	their superfast, broad-spectrum adsorption	76:117	their superfast, broad-spectrum adsorption towards pollutants	76:136	One-pot fabrication of antibacterial β-cyclodextrin-based nanoparticles and their superfast, broad-spectrum adsorption towards pollutants.					
32447020	7	64	theme	%	1292:1292	arg1	rate					1315:1318	99.99% bacterial inhibitive rate	1287:1318	99.99% bacterial inhibitive rate	1287:1318	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	7	65	theme	gram-positive	1387:1399	arg1	bacteria					1401:1408	gram-positive bacteria	1387:1408	gram-positive bacteria	1387:1408	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
32447020	7	66	theme	rate	1315:1318	arg1	activity					1275:1282	prominent antibacterial activity	1251:1282	prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus)	1251:1442	Interestingly, the resulting nanoparticles showed prominent antibacterial activity of 99.99% bacterial inhibitive rate against both gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus).					
34823760	0	0	theme	chitosan	111:118	arg1	derivative					120:129	chitosan derivative	111:129	chitosan derivative	111:129	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	5	1	theme	%	877:877	arg1	rates					854:858	antibacterial rates	840:858	antibacterial rates of 99.2% and 98.7%	840:877	The membrane achieved antibacterial rates of 99.2% and 98.7% against E. coli and S. aureus, respectively, without leaching antibacterial agents.					
34823760	5	2	theme	%	867:867	arg1	rates					854:858	antibacterial rates	840:858	antibacterial rates of 99.2% and 98.7%	840:877	The membrane achieved antibacterial rates of 99.2% and 98.7% against E. coli and S. aureus, respectively, without leaching antibacterial agents.					
34823760	4	3	theme	continuous	710:719	arg1	transport					758:766	continuous and spontaneous unidirectional water transport	710:766	continuous and spontaneous unidirectional water transport	710:766	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	6	4	theme	self-adhesive	1003:1015	arg1	properties					1017:1026	high elasticity and self-adhesive properties	983:1026	high elasticity and self-adhesive properties	983:1026	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	4	5	theme	%	815:815	arg1	R					805:805	R	805:805	R	805:805	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	4	5	theme	%	815:815	arg1	value					798:802	a high one-way transport value	773:802	a high one-way transport value (R) of 1483%	773:815	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	3	6	theme	mild	658:661	arg1	procedure					676:684	a mild crosslinking procedure	656:684	a mild crosslinking procedure	656:684	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	4	7	theme	spontaneous	725:735	arg1	transport					758:766	continuous and spontaneous unidirectional water transport	710:766	continuous and spontaneous unidirectional water transport	710:766	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	6	8	theme	elasticity	988:997	arg1	properties					1017:1026	high elasticity and self-adhesive properties	983:1026	high elasticity and self-adhesive properties	983:1026	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	3	9	theme	crosslinking	663:674	arg1	procedure					676:684	a mild crosslinking procedure	656:684	a mild crosslinking procedure	656:684	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	6	10	theme	applications	1069:1080	arg1	range					1060:1064	a range	1058:1064	a range of applications	1058:1080	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	6	11	theme	high	983:986	arg1	properties					1017:1026	high elasticity and self-adhesive properties	983:1026	high elasticity and self-adhesive properties	983:1026	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	5	12	theme	leaching	932:939	arg1	agents					955:960	leaching antibacterial agents	932:960	leaching antibacterial agents	932:960	The membrane achieved antibacterial rates of 99.2% and 98.7% against E. coli and S. aureus, respectively, without leaching antibacterial agents.					
34823760	1	13	theme	high-performance	214:229	arg1	fabrics					251:257	high-performance moisture-management fabrics	214:257	high-performance moisture-management fabrics for medical care	214:274	Unidirectional liquid transport is critical in achieving high-performance moisture-management fabrics for medical care.					
34823760	0	14	theme	composite	135:143	arg1	nanofibers					145:154	composite nanofibers	135:154	composite nanofibers	135:154	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	7	15	theme	dressings	1247:1255	arg1	field					1230:1234	the field	1226:1234	the field of medical dressings	1226:1255	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	2	16	theme	other	360:364	arg1	function					402:409	antibacterial function	388:409	antibacterial function	388:409	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	2	16	theme	other	360:364	arg1	requirements					366:377	other requirements	360:377	other requirements	360:377	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	1	17	theme	moisture-management	231:249	arg1	fabrics					251:257	high-performance moisture-management fabrics	214:257	high-performance moisture-management fabrics for medical care	214:274	Unidirectional liquid transport is critical in achieving high-performance moisture-management fabrics for medical care.					
34823760	0	18	theme	unidirectional	12:25	arg1	membrane					47:54	Stretchable unidirectional liquid-transporting membrane	0:54	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.	0:155	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	7	19	theme	novel	1178:1182	arg1	systems					1201:1207	novel moisture-wicking systems	1178:1207	novel moisture-wicking systems	1178:1207	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	0	20	theme	Stretchable	0:10	arg1	membrane					47:54	Stretchable unidirectional liquid-transporting membrane	0:54	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.	0:155	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	7	21	theme	moisture-wicking	1184:1199	arg1	systems					1201:1207	novel moisture-wicking systems	1178:1207	novel moisture-wicking systems	1178:1207	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	6	22	contain	has	979:981	arg1	it					976:977	it	976:977	it	976:977	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	6	22	contain	has	979:981	arg2	properties					1017:1026	high elasticity and self-adhesive properties	983:1026	high elasticity and self-adhesive properties	983:1026	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	3	23	theme	Janus	513:517	arg1	membranes					531:539	Janus nanofibrous membranes	513:539	Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity	513:614	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	2	24	theme	antibacterial	388:400	arg1	function					402:409	antibacterial function	388:409	antibacterial function	388:409	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	1	25	theme	medical	263:269	arg1	care					271:274	medical care	263:274	medical care	263:274	Unidirectional liquid transport is critical in achieving high-performance moisture-management fabrics for medical care.					
34823760	0	26	theme	liquid-transporting	27:45	arg1	membrane					47:54	Stretchable unidirectional liquid-transporting membrane	0:54	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.	0:155	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	7	27	theme	nanofibrous	1118:1128	arg1	membrane					1130:1137	this versatile Janus nanofibrous membrane	1097:1137	this versatile Janus nanofibrous membrane	1097:1137	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	3	28	theme	liquid	572:577	arg1	transport					579:587	unidirectional liquid transport	557:587	unidirectional liquid transport	557:587	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	0	29	theme	antibacterial	61:73	arg1	features					93:100	antibacterial and biocompatible features	61:100	antibacterial and biocompatible features based on chitosan derivative and composite nanofibers	61:154	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	2	30	theme	low	426:428	arg1	cytotoxicity					430:441	low cytotoxicity	426:441	low cytotoxicity	426:441	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	7	31	theme	new	1150:1152	arg1	strategy					1154:1161	a new strategy	1148:1161	a new strategy for developing novel moisture-wicking systems	1148:1207	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	5	32	theme	antibacterial	941:953	arg1	agents					955:960	leaching antibacterial agents	932:960	leaching antibacterial agents	932:960	The membrane achieved antibacterial rates of 99.2% and 98.7% against E. coli and S. aureus, respectively, without leaching antibacterial agents.					
34823760	2	33	theme	liquid	311:316	arg1	transport					318:326	unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function	296:409	unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function	296:409	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	4	34	theme	transport	788:796	arg1	R					805:805	R	805:805	R	805:805	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	4	34	theme	transport	788:796	arg1	value					798:802	a high one-way transport value	773:802	a high one-way transport value (R) of 1483%	773:815	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	3	35	theme	nanofibrous	519:529	arg1	membranes					531:539	Janus nanofibrous membranes	513:539	Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity	513:614	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	5	36	theme	antibacterial	840:852	arg1	rates					854:858	antibacterial rates	840:858	antibacterial rates of 99.2% and 98.7%	840:877	The membrane achieved antibacterial rates of 99.2% and 98.7% against E. coli and S. aureus, respectively, without leaching antibacterial agents.					
34823760	2	37	theme	unidirectional	296:309	arg1	transport					318:326	unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function	296:409	unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function	296:409	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	0	38	with	membrane	47:54	arg1	features					93:100	antibacterial and biocompatible features	61:100	antibacterial and biocompatible features based on chitosan derivative and composite nanofibers	61:154	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	4	39	theme	one-way	780:786	arg1	R					805:805	R	805:805	R	805:805	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	4	39	theme	one-way	780:786	arg1	value					798:802	a high one-way transport value	773:802	a high one-way transport value (R) of 1483%	773:815	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	0	40	theme	biocompatible	79:91	arg1	features					93:100	antibacterial and biocompatible features	61:100	antibacterial and biocompatible features based on chitosan derivative and composite nanofibers	61:154	Stretchable unidirectional liquid-transporting membrane with antibacterial and biocompatible features based on chitosan derivative and composite nanofibers.					
34823760	7	41	theme	Janus	1112:1116	arg1	membrane					1130:1137	this versatile Janus nanofibrous membrane	1097:1137	this versatile Janus nanofibrous membrane	1097:1137	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	3	42	theme	antibacterial	593:605	arg1	activity					607:614	antibacterial activity	593:614	antibacterial activity	593:614	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	7	43	theme	medical	1239:1245	arg1	dressings					1247:1255	medical dressings	1239:1255	medical dressings	1239:1255	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	1	44	theme	Unidirectional	157:170	arg1	transport					179:187	Unidirectional liquid transport	157:187	Unidirectional liquid transport	157:187	Unidirectional liquid transport is critical in achieving high-performance moisture-management fabrics for medical care.					
34823760	6	45	from	use	1051:1053	arg1	range					1060:1064	a range	1058:1064	a range of applications	1058:1080	In addition, it has high elasticity and self-adhesive properties, which facilitates its use in a range of applications.					
34823760	1	46	theme	liquid	172:177	arg1	transport					179:187	Unidirectional liquid transport	157:187	Unidirectional liquid transport	157:187	Unidirectional liquid transport is critical in achieving high-performance moisture-management fabrics for medical care.					
34823760	4	47	theme	water	752:756	arg1	transport					758:766	continuous and spontaneous unidirectional water transport	710:766	continuous and spontaneous unidirectional water transport	710:766	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	7	48	theme	membrane	1130:1137	arg1	design					1087:1092	The design	1083:1092	The design of this versatile Janus nanofibrous membrane	1083:1137	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	7	49	theme	versatile	1102:1110	arg1	membrane					1130:1137	this versatile Janus nanofibrous membrane	1097:1137	this versatile Janus nanofibrous membrane	1097:1137	The design of this versatile Janus nanofibrous membrane provides a new strategy for developing novel moisture-wicking systems, particularly in the field of medical dressings.					
34823760	4	50	theme	unidirectional	737:750	arg1	transport					758:766	continuous and spontaneous unidirectional water transport	710:766	continuous and spontaneous unidirectional water transport	710:766	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	3	51	theme	unidirectional	557:570	arg1	transport					579:587	unidirectional liquid transport	557:587	unidirectional liquid transport	557:587	In this study, Janus nanofibrous membranes exhibiting both unidirectional liquid transport and antibacterial activity were fabricated via electrospinning and a mild crosslinking procedure.					
34823760	2	52	theme	mechanical	457:466	arg1	strength					468:475	adequate mechanical strength	448:475	adequate mechanical strength	448:475	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
34823760	4	53	theme	high	775:778	arg1	R					805:805	R	805:805	R	805:805	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	4	53	theme	high	775:778	arg1	value					798:802	a high one-way transport value	773:802	a high one-way transport value (R) of 1483%	773:815	This membrane provides continuous and spontaneous unidirectional water transport with a high one-way transport value (R) of 1483%.					
34823760	2	54	theme	adequate	448:455	arg1	strength					468:475	adequate mechanical strength	448:475	adequate mechanical strength	448:475	However, realizing unidirectional liquid transport while simultaneously satisfying other requirements, such as antibacterial function, adhesiveness, low cytotoxicity, and adequate mechanical strength remains a challenge.					
33538616	5	0	theme	optimized	819:827	arg1	nanoparticles					840:852	the optimized EPO-loaded nanoparticles	815:852	the optimized EPO-loaded nanoparticles	815:852	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	6	1	theme	room	993:996	arg1	temperature					998:1008	room temperature	993:1008	room temperature	993:1008	The hydrogel containing nanoparticles existed as a solution at room temperature converted to a semisolid upon increasing the temperature to 35 ± 1.2 °C and demonstrated controlled release of EPO for more than 10 days.					
33538616	7	2	theme	standard	1325:1332	arg1	EPO					1334:1336	standard EPO	1325:1336	standard EPO	1325:1336	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	0	3	theme	in vitro	87:94	arg1	studies					96:102	in vitro studies	87:102	in vitro studies	87:102	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	1	4	theme	current	155:161	arg1	study					163:167	the current study erythropoietin (EPO)	151:188	the current study erythropoietin (EPO)	151:188	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	6	5	theme	containing	943:952	arg1	nanoparticles					954:966	The hydrogel containing nanoparticles	930:966	The hydrogel containing nanoparticles	930:966	The hydrogel containing nanoparticles existed as a solution at room temperature converted to a semisolid upon increasing the temperature to 35 ± 1.2 °C and demonstrated controlled release of EPO for more than 10 days.					
33538616	0	6	theme	biological	105:114	arg1	activity					116:123	biological activity	105:123	biological activity	105:123	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	3	7	theme	freeze	650:655	arg1	drying					657:662	freeze drying	650:662	freeze drying	650:662	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	7	8	theme	0.85	1301:1304	arg1	potency					1290:1296	relative potency	1281:1296	relative potency of 0.85	1281:1304	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	2	9	theme	main	321:324	arg1	factors					339:345	the main experimental factors	317:345	the main experimental factors	317:345	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	4	10	theme	Rheological	665:675	arg1	properties					677:686	Rheological properties	665:686	Rheological properties	665:686	Rheological properties, and the release rate of EPO from the hydrogel were examined.					
33538616	7	11	theme	relative	1281:1288	arg1	potency					1290:1296	relative potency	1281:1296	relative potency of 0.85	1281:1304	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	4	12	theme	release	697:703	arg1	rate					705:708	the release rate	693:708	the release rate of EPO from the hydrogel	693:733	Rheological properties, and the release rate of EPO from the hydrogel were examined.					
33538616	7	13	theme	biological	1231:1240	arg1	assay					1251:1255	in vivo biological activity assay	1223:1255	in vivo biological activity assay	1223:1255	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	6	14	theme	controlled	1099:1108	arg1	release					1110:1116	controlled release	1099:1116	controlled release of EPO	1099:1123	The hydrogel containing nanoparticles existed as a solution at room temperature converted to a semisolid upon increasing the temperature to 35 ± 1.2 °C and demonstrated controlled release of EPO for more than 10 days.					
33538616	5	15	theme	entrapment	790:799	arg1	efficiency					801:810	entrapment efficiency	790:810	entrapment efficiency	790:810	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	3	16	theme	circular	538:545	arg1	dichroismspectroscopy					547:567	circular dichroismspectroscopy	538:567	circular dichroismspectroscopy	538:567	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	3	17	theme	dodecyl	513:519	arg1	sulfate-page					521:532	Sodium dodecyl sulfate-page	506:532	Sodium dodecyl sulfate-page	506:532	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	4	18	from	hydrogel	726:733	arg1	rate					705:708	the release rate	693:708	the release rate of EPO from the hydrogel	693:733	Rheological properties, and the release rate of EPO from the hydrogel were examined.					
33538616	4	18	from	hydrogel	726:733	arg1	properties					677:686	Rheological properties	665:686	Rheological properties	665:686	Rheological properties, and the release rate of EPO from the hydrogel were examined.					
33538616	2	19	theme	composite	436:444	arg1	design					446:451	a two-factors central composite design	414:451	a two-factors central composite design	414:451	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	7	20	theme	in vivo	1223:1229	arg1	assay					1251:1255	in vivo biological activity assay	1223:1255	in vivo biological activity assay	1223:1255	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	5	21	theme	nanoparticles	840:852	arg1	efficiency					801:810	entrapment efficiency	790:810	entrapment efficiency	790:810	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	5	21	theme	nanoparticles	840:852	arg1	size					764:767	Mean particle size	750:767	Mean particle size	750:767	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	5	21	theme	nanoparticles	840:852	arg1	potential					775:783	zeta potential	770:783	zeta potential	770:783	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	3	22	theme	structural	594:603	arg1	stability					605:613	the structural stability	590:613	the structural stability of EPO	590:620	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	2	23	theme	central	428:434	arg1	design					446:451	a two-factors central composite design	414:451	a two-factors central composite design	414:451	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	5	24	theme	EPO-loaded	829:838	arg1	nanoparticles					840:852	the optimized EPO-loaded nanoparticles	815:852	the optimized EPO-loaded nanoparticles	815:852	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	7	25	theme	EPO	1165:1167	arg1	stability					1152:1160	The stability	1148:1160	The stability of EPO in the hydrogel system	1148:1190	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	8	26	theme	elevated	1472:1479	arg1	reticulocytes					1481:1493	elevated reticulocytes	1472:1493	elevated reticulocytes for about 20 days compared to control group received blank hydrogel	1472:1561	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	2	27	theme	two-factors	416:426	arg1	design					446:451	a two-factors central composite design	414:451	a two-factors central composite design	414:451	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	2	28	theme	factors	339:345	arg1	influence					304:312	The influence	300:312	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles	300:391	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	6	29	theme	hydrogel	934:941	arg1	nanoparticles					954:966	The hydrogel containing nanoparticles	930:966	The hydrogel containing nanoparticles	930:966	The hydrogel containing nanoparticles existed as a solution at room temperature converted to a semisolid upon increasing the temperature to 35 ± 1.2 °C and demonstrated controlled release of EPO for more than 10 days.					
33538616	2	30	theme	experimental	326:337	arg1	factors					339:345	the main experimental factors	317:345	the main experimental factors	317:345	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	2	31	theme	optimized	461:469	arg1	formulation					471:481	the optimized formulation	457:481	the optimized formulation	457:481	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	2	31	theme	optimized	461:469	arg1	dried					499:503	dried	499:503	dried	499:503	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	0	32	theme	erythropoietin	21:34	arg1	Sustained-release					0:16	Sustained-release	0:16	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.	0:146	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	0	33	from	studies	96:102	arg1	rats					142:145	rats	142:145	rats	142:145	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	2	34	theme	nanoparticles	379:391	arg1	properties					354:363	the properties	350:363	the properties of EPO-loaded nanoparticles	350:391	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	1	35	theme	loaded	190:195	arg1	nanoparticles-embedded					233:254	loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded	190:254	loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel	190:284	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	0	36	theme	novel	44:48	arg1	hydrogel					77:84	a novel injectable thermosensitive hydrogel	42:84	a novel injectable thermosensitive hydrogel	42:84	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	5	37	theme	zeta	770:773	arg1	potential					775:783	zeta potential	770:783	zeta potential	770:783	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	4	38	theme	EPO	713:715	arg1	rate					705:708	the release rate	693:708	the release rate of EPO from the hydrogel	693:733	Rheological properties, and the release rate of EPO from the hydrogel were examined.					
33538616	4	38	theme	EPO	713:715	arg1	properties					677:686	Rheological properties	665:686	Rheological properties	665:686	Rheological properties, and the release rate of EPO from the hydrogel were examined.					
33538616	1	39	theme	trimethyl	197:205	arg1	nanoparticles-embedded					233:254	loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded	190:254	loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel	190:284	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	7	40	theme	activity	1242:1249	arg1	assay					1251:1255	in vivo biological activity assay	1223:1255	in vivo biological activity assay	1223:1255	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	1	41	theme	chitosan/tripolyphosphate	207:231	arg1	nanoparticles-embedded					233:254	loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded	190:254	loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel	190:284	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	0	42	theme	thermosensitive	61:75	arg1	hydrogel					77:84	a novel injectable thermosensitive hydrogel	42:84	a novel injectable thermosensitive hydrogel	42:84	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	8	43	theme	Sprague-Dawley	1440:1453	arg1	rats					1455:1458	Sprague-Dawley rats	1440:1458	Sprague-Dawley rats	1440:1458	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	1	44	dep	study	163:167	arg1	EPO					185:187	EPO	185:187	EPO	185:187	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	1	44	dep	study	163:167	arg1	erythropoietin					169:182	erythropoietin	169:182	the current study erythropoietin (EPO)	151:188	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	5	45	theme	Mean	750:753	arg1	size					764:767	Mean particle size	750:767	Mean particle size	750:767	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	2	46	theme	EPO-loaded	368:377	arg1	nanoparticles					379:391	EPO-loaded nanoparticles	368:391	EPO-loaded nanoparticles	368:391	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	0	47	theme	injectable	50:59	arg1	hydrogel					77:84	a novel injectable thermosensitive hydrogel	42:84	a novel injectable thermosensitive hydrogel	42:84	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	7	48	theme	hydrogel	1176:1183	arg1	system					1185:1190	the hydrogel system	1172:1190	the hydrogel system	1172:1190	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	2	49	from	influence	304:312	arg1	properties					354:363	the properties	350:363	the properties of EPO-loaded nanoparticles	350:391	The influence of the main experimental factors on the properties of EPO-loaded nanoparticles were evaluated using a two-factors central composite design and the optimized formulation was then freeze dried.					
33538616	1	50	from	nanoparticles-embedded	233:254	arg1	hydrogel					277:284	a thermosensitive hydrogel	259:284	a thermosensitive hydrogel	259:284	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
33538616	0	51	from	activity	116:123	arg1	rats					142:145	rats	142:145	rats	142:145	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	3	52	used	used	574:577	arg2	sulfate-page					521:532	Sodium dodecyl sulfate-page	506:532	Sodium dodecyl sulfate-page	506:532	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	3	52	used	used	574:577	arg2	dichroismspectroscopy					547:567	circular dichroismspectroscopy	538:567	circular dichroismspectroscopy	538:567	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	8	53	theme	nanoparticles-embedded	1385:1406	arg1	injection					1357:1365	a single injection	1348:1365	a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats	1348:1458	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	3	54	theme	EPO	618:620	arg1	stability					605:613	the structural stability	590:613	the structural stability of EPO	590:620	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	6	55	theme	EPO	1121:1123	arg1	release					1110:1116	controlled release	1099:1116	controlled release of EPO	1099:1123	The hydrogel containing nanoparticles existed as a solution at room temperature converted to a semisolid upon increasing the temperature to 35 ± 1.2 °C and demonstrated controlled release of EPO for more than 10 days.					
33538616	5	56	theme	particle	755:762	arg1	size					764:767	Mean particle size	750:767	Mean particle size	750:767	Mean particle size, zeta potential, and entrapment efficiency of the optimized EPO-loaded nanoparticles were confirmed 151.5 ± 16 nm, 11.5 ± 1.8 mV, and 78.5 ± 5.9%, respectively.					
33538616	8	57	theme	control	1525:1531	arg1	group					1533:1537	control group	1525:1537	control group received blank hydrogel	1525:1561	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	8	58	from	injection	1357:1365	arg1	hydrogel					1415:1422	the hydrogel	1411:1422	the hydrogel administered to Sprague-Dawley rats	1411:1458	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	8	59	theme	EPO-loaded	1374:1383	arg1	nanoparticles-embedded					1385:1406	the EPO-loaded nanoparticles-embedded	1370:1406	the EPO-loaded nanoparticles-embedded	1370:1406	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	8	60	theme	single	1350:1355	arg1	injection					1357:1365	a single injection	1348:1365	a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats	1348:1458	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	8	61	theme	blank	1548:1552	arg1	hydrogel					1554:1561	blank hydrogel	1548:1561	blank hydrogel	1548:1561	Finally, a single injection of the EPO-loaded nanoparticles-embedded in the hydrogel administered to Sprague-Dawley rats resulted in elevated reticulocytes for about 20 days compared to control group received blank hydrogel.					
33538616	3	62	theme	Sodium	506:511	arg1	sulfate-page					521:532	Sodium dodecyl sulfate-page	506:532	Sodium dodecyl sulfate-page	506:532	Sodium dodecyl sulfate-page and circular dichroismspectroscopy were used to confirm the structural stability of EPO following encapsulation and freeze drying.					
33538616	0	63	from	efficacy	130:137	arg1	rats					142:145	rats	142:145	rats	142:145	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	7	64	from	stability	1152:1160	arg1	system					1185:1190	the hydrogel system	1172:1190	the hydrogel system	1172:1190	The stability of EPO in the hydrogel system was further investigated using in vivo biological activity assay and the result revealed relative potency of 0.85 as calibrated with standard EPO.					
33538616	0	65	dep	Sustained-release	0:16	arg1	studies					96:102	in vitro studies	87:102	in vitro studies	87:102	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	0	65	dep	Sustained-release	0:16	arg1	activity					116:123	biological activity	105:123	biological activity	105:123	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	0	65	dep	Sustained-release	0:16	arg1	efficacy					130:137	efficacy	130:137	efficacy	130:137	Sustained-release of erythropoietin using a novel injectable thermosensitive hydrogel: in vitro studies, biological activity, and efficacy in rats.					
33538616	6	66	from	temperature	998:1008	arg1	solution					981:988	a solution	979:988	a solution at room temperature	979:1008	The hydrogel containing nanoparticles existed as a solution at room temperature converted to a semisolid upon increasing the temperature to 35 ± 1.2 °C and demonstrated controlled release of EPO for more than 10 days.					
33538616	1	67	theme	thermosensitive	261:275	arg1	hydrogel					277:284	a thermosensitive hydrogel	259:284	a thermosensitive hydrogel	259:284	In the current study erythropoietin (EPO) loaded trimethyl chitosan/tripolyphosphate nanoparticles-embedded in a thermosensitive hydrogel was prepared.					
34791616	6	0	theme	atlantica	934:942	arg1	oil					950:952	the Pistacia atlantica fruit oil	921:952	the Pistacia atlantica fruit oil	921:952	Bacterial cellulose membrane was impregnated with the Pistacia atlantica fruit oil.					
34791616	5	1	used	used	762:765	arg2	technique					748:756	Cold press technique	737:756	Cold press technique	737:756	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	10	2	theme	FT-IR	1451:1455	arg1	spectrum					1457:1464	the standard FT-IR spectrum	1438:1464	the standard FT-IR spectrum	1438:1464	RESULTS FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum.					
34791616	8	3	theme	Pistacia	1193:1200	arg1	hydrogel					1212:1219	Pistacia atlantica hydrogel	1193:1219	Pistacia atlantica hydrogel	1193:1219	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	12	4	theme	negative	1773:1780	arg1	control					1782:1788	negative control	1773:1788	negative control	1773:1788	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	11	5	theme	major	1471:1475	arg1	components					1477:1486	The major components	1467:1486	The major components of Pistacia atlantica fruit oil	1467:1518	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	3	6	theme	bacterial	533:541	arg1	cellulose					543:551	oil-absorbed bacterial cellulose	520:551	oil-absorbed bacterial cellulose	520:551	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	2	7	from	Medicine	326:333	arg1	treatment					249:257	treatment	249:257	treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM)	249:339	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	8	8	theme	fruit	1288:1292	arg1	oil					1294:1296	the Pistacia atlantica fruit oil	1265:1296	the Pistacia atlantica fruit oil	1265:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	2	9	theme	wounds	262:267	arg1	treatment					249:257	treatment	249:257	treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM)	249:339	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	1	10	theme	few	191:193	arg1	decades					195:201	few decades	191:201	few decades	191:201	BACKGROUND Biological activities of Pistacia atlantica have been investigated for few decades.					
34791616	8	11	theme	bacterial	1224:1232	arg1	membrane					1244:1251	bacterial cellulose membrane	1224:1251	bacterial cellulose membrane coated with the Pistacia atlantica fruit oil	1224:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	8	12	theme	wound	1026:1030	arg1	model					1032:1036	Burn wound model	1021:1036	Burn wound model	1021:1036	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	4	13	theme	Fourier-transform	660:676	arg1	infrared					678:685	Fourier-transform infrared	660:685	Fourier-transform infrared	660:685	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	2	14	theme	ailments	294:301	arg1	treatment					249:257	treatment	249:257	treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM)	249:339	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	12	15	theme	Histological	1599:1610	arg1	analysis					1612:1619	Histological analysis	1599:1619	Histological analysis	1599:1619	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	8	16	used	used	1042:1045	arg2	model					1032:1036	Burn wound model	1021:1036	Burn wound model	1021:1036	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	11	17	theme	oil	1516:1518	arg1	components					1477:1486	The major components	1467:1486	The major components of Pistacia atlantica fruit oil	1467:1518	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	5	18	theme	chemical	814:821	arg1	composition					823:833	the chemical composition	810:833	the chemical composition	810:833	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	5	19	theme	gas	851:853	arg1	chromatography					855:868	gas chromatography	851:868	gas chromatography	851:868	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	8	20	theme	Pistacia	1269:1276	arg1	oil					1294:1296	the Pistacia atlantica fruit oil	1265:1296	the Pistacia atlantica fruit oil	1265:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	14	21	theme	wound	2097:2101	arg1	management					2103:2112	wound management	2097:2112	wound management	2097:2112	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	9	22	theme	Blank	1299:1303	arg1	control					1335:1341	negative control	1326:1341	negative control	1326:1341	Blank dressing was used as negative control.					
34791616	9	22	theme	Blank	1299:1303	arg1	dressing					1305:1312	Blank dressing	1299:1312	Blank dressing	1299:1312	Blank dressing was used as negative control.					
34791616	3	23	theme	burn	567:570	arg1	model					578:582	an in vivo burn wound model	556:582	an in vivo burn wound model	556:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	10	24	theme	standard	1442:1449	arg1	spectrum					1457:1464	the standard FT-IR spectrum	1438:1464	the standard FT-IR spectrum	1438:1464	RESULTS FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum.					
34791616	13	25	theme	other	1931:1935	arg1	p < 0.05					1949:1956	p < 0.05	1949:1956	p < 0.05	1949:1956	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	13	25	theme	other	1931:1935	arg1	treatments					1937:1946	the other treatments	1927:1946	the other treatments (p < 0.05)	1927:1957	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	1	26	theme	Pistacia	145:152	arg1	atlantica					154:162	Pistacia atlantica	145:162	Pistacia atlantica	145:162	BACKGROUND Biological activities of Pistacia atlantica have been investigated for few decades.					
34791616	2	27	from	wounds	262:267	arg1	Medicine					326:333	Traditional Persian Medicine	306:333	Traditional Persian Medicine	306:333	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	7	28	theme	specific	1002:1009	arg1	Carbopol					1011:1018	specific Carbopol	1002:1018	specific Carbopol	1002:1018	Pistacia atlantica hydrogel was prepared using specific Carbopol.					
34791616	0	29	theme	Pistacia	79:86	arg1	oil					104:106	Pistacia atlantica fruit oil	79:106	Pistacia atlantica fruit oil	79:106	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	3	30	dep	in	559:560	arg1	vivo					562:565	vivo	562:565	vivo	562:565	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	12	31	theme	neutrophils	1713:1723	arg1	measure					1730:1736	a measure	1728:1736	a measure of inflammation	1728:1752	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	12	31	theme	neutrophils	1713:1723	arg1	number					1703:1708	the number	1699:1708	the number of neutrophils	1699:1723	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	8	32	dep	Pistacia	1193:1200	arg1	atlantica					1202:1210	atlantica	1202:1210	atlantica	1202:1210	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	12	33	theme	positive	1793:1800	arg1	p < 0.05					1811:1818	p < 0.05	1811:1818	p < 0.05	1811:1818	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	12	33	theme	positive	1793:1800	arg1	control					1802:1808	positive control	1793:1808	positive control (p < 0.05)	1793:1819	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	14	34	theme	bacterial	1995:2003	arg1	dressing					2084:2091	a potential bio-safe dressing	2063:2091	a potential bio-safe dressing for wound management	2063:2112	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	14	34	theme	bacterial	1995:2003	arg1	cellulose					2005:2013	bacterial cellulose	1995:2013	bacterial cellulose coated with Pistacia atlantica fruit oil	1995:2054	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	0	35	theme	fruit	98:102	arg1	oil					104:106	Pistacia atlantica fruit oil	79:106	Pistacia atlantica fruit oil	79:106	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	2	36	theme	inflammation	270:281	arg1	treatment					249:257	treatment	249:257	treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM)	249:339	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	11	37	theme	fruit	1510:1514	arg1	oil					1516:1518	Pistacia atlantica fruit oil	1491:1518	Pistacia atlantica fruit oil	1491:1518	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	11	38	theme	atlantica	1500:1508	arg1	oil					1516:1518	Pistacia atlantica fruit oil	1491:1518	Pistacia atlantica fruit oil	1491:1518	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	3	39	theme	chemical	407:414	arg1	composition					416:426	the chemical composition	403:426	the chemical composition of Pistacia atlantica fruit oil	403:458	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	8	40	theme	wound	1067:1071	arg1	healing					1073:1079	wound healing	1067:1079	wound healing	1067:1079	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	4	41	used	used	691:694	arg2	infrared					678:685	Fourier-transform infrared	660:685	Fourier-transform infrared	660:685	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	14	42	theme	Pistacia	2027:2034	arg1	oil					2052:2054	Pistacia atlantica fruit oil	2027:2054	Pistacia atlantica fruit oil	2027:2054	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	13	43	theme	oil-coated	1884:1893	arg1	cellulose					1905:1913	fruit oil-coated bacterial cellulose	1878:1913	fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05)	1878:1957	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	0	44	theme	Wound	0:4	arg1	healing					6:12	Wound healing	0:12	Wound healing	0:12	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	8	45	dep	Pistacia	1269:1276	arg1	atlantica					1278:1286	atlantica	1278:1286	atlantica	1278:1286	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	11	46	theme	linoleic	1532:1539	arg1	acid					1541:1544	linoleic acid	1532:1544	linoleic acid (38.1%)	1532:1552	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	11	46	theme	linoleic	1532:1539	arg1	%					1551:1551	38.1%	1547:1551	38.1%	1547:1551	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	3	47	theme	atlantica	440:448	arg1	oil					456:458	Pistacia atlantica fruit oil	431:458	Pistacia atlantica fruit oil	431:458	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	6	48	theme	cellulose	881:889	arg1	membrane					891:898	Bacterial cellulose membrane	871:898	Bacterial cellulose membrane	871:898	Bacterial cellulose membrane was impregnated with the Pistacia atlantica fruit oil.					
34791616	1	49	theme	BACKGROUND	109:118	arg1	activities					131:140	BACKGROUND Biological activities	109:140	BACKGROUND Biological activities of Pistacia atlantica	109:162	BACKGROUND Biological activities of Pistacia atlantica have been investigated for few decades.					
34791616	10	50	theme	RESULTS	1344:1350	arg1	analysis					1358:1365	RESULTS FT-IR analysis	1344:1365	RESULTS FT-IR analysis	1344:1365	RESULTS FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum.					
34791616	5	51	theme	atlantica	786:794	arg1	oil					802:804	Pistacia atlantica fruit oil	777:804	Pistacia atlantica fruit oil	777:804	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	14	52	theme	potential	2065:2073	arg1	dressing					2084:2091	a potential bio-safe dressing	2063:2091	a potential bio-safe dressing for wound management	2063:2112	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	14	52	theme	potential	2065:2073	arg1	cellulose					2005:2013	bacterial cellulose	1995:2013	bacterial cellulose coated with Pistacia atlantica fruit oil	1995:2054	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	0	53	theme	bacterial	47:55	arg1	cellulose					57:65	bacterial cellulose	47:65	bacterial cellulose	47:65	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	8	54	theme	dressings	1124:1132	arg1	healing					1073:1079	wound healing	1067:1079	wound healing	1067:1079	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	8	54	theme	dressings	1124:1132	arg1	effects					1103:1109	anti-inflammatory effects	1085:1109	anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil	1085:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	2	55	theme	Persian	318:324	arg1	Medicine					326:333	Traditional Persian Medicine	306:333	Traditional Persian Medicine	306:333	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	10	56	theme	cellulose	1410:1418	arg1	structure					1383:1391	the structure	1379:1391	the structure of the bacterial cellulose	1379:1418	RESULTS FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum.					
34791616	5	57	theme	fruit	796:800	arg1	oil					802:804	Pistacia atlantica fruit oil	777:804	Pistacia atlantica fruit oil	777:804	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	6	58	theme	fruit	944:948	arg1	oil					950:952	the Pistacia atlantica fruit oil	921:952	the Pistacia atlantica fruit oil	921:952	Bacterial cellulose membrane was impregnated with the Pistacia atlantica fruit oil.					
34791616	4	59	theme	Bacterial	592:600	arg1	membrane					612:619	METHOD Bacterial cellulose membrane	585:619	METHOD Bacterial cellulose membrane	585:619	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	14	60	dep	CONCLUSION	1960:1969	arg1	showed					1983:1988	showed	1983:1988	showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management	1983:2112	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	3	61	theme	wound	473:477	arg1	healing					479:485	wound healing	473:485	wound healing	473:485	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	13	62	theme	bacterial	1895:1903	arg1	cellulose					1905:1913	fruit oil-coated bacterial cellulose	1878:1913	fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05)	1878:1957	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	8	63	theme	positive	1175:1182	arg1	control					1184:1190	positive control	1175:1190	positive control	1175:1190	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	6	64	theme	Pistacia	925:932	arg1	oil					950:952	the Pistacia atlantica fruit oil	921:952	the Pistacia atlantica fruit oil	921:952	Bacterial cellulose membrane was impregnated with the Pistacia atlantica fruit oil.					
34791616	12	65	theme	inflammation	1741:1752	arg1	measure					1730:1736	a measure	1728:1736	a measure of inflammation	1728:1752	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	12	65	theme	inflammation	1741:1752	arg1	number					1703:1708	the number	1699:1708	the number of neutrophils	1699:1723	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	3	66	from	healing	479:485	arg1	model					578:582	an in vivo burn wound model	556:582	an in vivo burn wound model	556:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	3	67	theme	oil-absorbed	520:531	arg1	cellulose					543:551	oil-absorbed bacterial cellulose	520:551	oil-absorbed bacterial cellulose	520:551	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	8	68	theme	Burn	1021:1024	arg1	model					1032:1036	Burn wound model	1021:1036	Burn wound model	1021:1036	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	2	69	used	used	240:243	arg2	oil					214:216	The fruit oil	204:216	The fruit oil of the plant	204:229	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	4	70	theme	Kombucha	639:646	arg1	culture					648:654	Kombucha culture	639:654	Kombucha culture	639:654	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	11	71	theme	stearic	1578:1584	arg1	acid					1586:1589	stearic acid	1578:1589	stearic acid (3.8%)	1578:1596	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	11	71	theme	stearic	1578:1584	arg1	%					1595:1595	3.8%	1592:1595	3.8%	1592:1595	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	3	72	theme	cellulose	543:551	arg1	healing					479:485	wound healing	473:485	wound healing	473:485	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	3	72	theme	cellulose	543:551	arg1	effects					509:515	anti-inflammatory effects	491:515	anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model	491:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	8	73	theme	cellulose	1234:1242	arg1	membrane					1244:1251	bacterial cellulose membrane	1224:1251	bacterial cellulose membrane coated with the Pistacia atlantica fruit oil	1224:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	3	74	theme	wound	572:576	arg1	model					578:582	an in vivo burn wound model	556:582	an in vivo burn wound model	556:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	2	75	theme	plant	225:229	arg1	oil					214:216	The fruit oil	204:216	The fruit oil of the plant	204:229	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	1	76	theme	Biological	120:129	arg1	activities					131:140	BACKGROUND Biological activities	109:140	BACKGROUND Biological activities of Pistacia atlantica	109:162	BACKGROUND Biological activities of Pistacia atlantica have been investigated for few decades.					
34791616	2	77	theme	other	288:292	arg1	ailments					294:301	other ailments	288:301	other ailments in Traditional Persian Medicine (TPM)	288:339	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	2	77	theme	other	288:292	arg1	TPM					336:338	TPM	336:338	TPM	336:338	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	4	78	theme	bacterial	716:724	arg1	cellulose					726:734	the bacterial cellulose	712:734	the bacterial cellulose	712:734	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	3	79	theme	oil	456:458	arg1	composition					416:426	the chemical composition	403:426	the chemical composition of Pistacia atlantica fruit oil	403:458	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	1	80	theme	atlantica	154:162	arg1	activities					131:140	BACKGROUND Biological activities	109:140	BACKGROUND Biological activities of Pistacia atlantica	109:162	BACKGROUND Biological activities of Pistacia atlantica have been investigated for few decades.					
34791616	2	81	from	inflammation	270:281	arg1	Medicine					326:333	Traditional Persian Medicine	306:333	Traditional Persian Medicine	306:333	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	3	82	theme	in	559:560	arg1	model					578:582	an in vivo burn wound model	556:582	an in vivo burn wound model	556:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	3	83	from	effects	509:515	arg1	model					578:582	an in vivo burn wound model	556:582	an in vivo burn wound model	556:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	5	84	theme	Cold	737:740	arg1	technique					748:756	Cold press technique	737:756	Cold press technique	737:756	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	0	85	theme	atlantica	88:96	arg1	oil					104:106	Pistacia atlantica fruit oil	79:106	Pistacia atlantica fruit oil	79:106	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	9	86	theme	negative	1326:1333	arg1	control					1335:1341	negative control	1326:1341	negative control	1326:1341	Blank dressing was used as negative control.					
34791616	9	86	theme	negative	1326:1333	arg1	dressing					1305:1312	Blank dressing	1299:1312	Blank dressing	1299:1312	Blank dressing was used as negative control.					
34791616	2	87	from	ailments	294:301	arg1	Medicine					326:333	Traditional Persian Medicine	306:333	Traditional Persian Medicine	306:333	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	14	88	theme	fruit	2046:2050	arg1	oil					2052:2054	Pistacia atlantica fruit oil	2027:2054	Pistacia atlantica fruit oil	2027:2054	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	11	89	theme	Pistacia	1491:1498	arg1	oil					1516:1518	Pistacia atlantica fruit oil	1491:1518	Pistacia atlantica fruit oil	1491:1518	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	2	90	theme	Traditional	306:316	arg1	Medicine					326:333	Traditional Persian Medicine	306:333	Traditional Persian Medicine	306:333	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	5	91	theme	press	742:746	arg1	technique					748:756	Cold press technique	737:756	Cold press technique	737:756	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	14	92	theme	atlantica	2036:2044	arg1	oil					2052:2054	Pistacia atlantica fruit oil	2027:2054	Pistacia atlantica fruit oil	2027:2054	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	12	93	theme	fruit	1665:1669	arg1	oil					1671:1673	fruit oil	1665:1673	fruit oil	1665:1673	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	11	94	theme	oleic	1555:1559	arg1	%					1571:1571	36.9%	1567:1571	36.9%	1567:1571	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	11	94	theme	oleic	1555:1559	arg1	acid					1561:1564	oleic acid	1555:1564	oleic acid (36.9%)	1555:1572	The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%).					
34791616	13	95	theme	treated	1859:1865	arg1	group					1867:1871	the treated group	1855:1871	the treated group	1855:1871	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	13	96	theme	fruit	1878:1882	arg1	cellulose					1905:1913	fruit oil-coated bacterial cellulose	1878:1913	fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05)	1878:1957	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	8	97	theme	anti-inflammatory	1085:1101	arg1	effects					1103:1109	anti-inflammatory effects	1085:1109	anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil	1085:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	7	98	theme	atlantica	964:972	arg1	hydrogel					974:981	Pistacia atlantica hydrogel	955:981	Pistacia atlantica hydrogel	955:981	Pistacia atlantica hydrogel was prepared using specific Carbopol.					
34791616	0	99	theme	anti-inflammatory	18:34	arg1	effects					36:42	anti-inflammatory effects	18:42	anti-inflammatory effects of bacterial cellulose	18:65	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	9	100	used	used	1318:1321	arg2	dressing					1305:1312	Blank dressing	1299:1312	Blank dressing	1299:1312	Blank dressing was used as negative control.					
34791616	9	100	used	used	1318:1321	arg2	control					1335:1341	negative control	1326:1341	negative control	1326:1341	Blank dressing was used as negative control.					
34791616	12	101	theme	bacterial	1633:1641	arg1	cellulose					1643:1651	bacterial cellulose	1633:1651	bacterial cellulose coated with fruit oil	1633:1673	Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05).					
34791616	3	102	theme	main	357:360	arg1	objectives					362:371	The main objectives	353:371	The main objectives of this study	353:385	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	10	103	theme	FT-IR	1352:1356	arg1	analysis					1358:1365	RESULTS FT-IR analysis	1344:1365	RESULTS FT-IR analysis	1344:1365	RESULTS FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum.					
34791616	3	104	theme	fruit	450:454	arg1	oil					456:458	Pistacia atlantica fruit oil	431:458	Pistacia atlantica fruit oil	431:458	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	5	105	theme	Pistacia	777:784	arg1	oil					802:804	Pistacia atlantica fruit oil	777:804	Pistacia atlantica fruit oil	777:804	Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography.					
34791616	8	106	theme	wound	1118:1122	arg1	dressings					1124:1132	the wound dressings	1114:1132	the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil	1114:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	6	107	theme	Bacterial	871:879	arg1	membrane					891:898	Bacterial cellulose membrane	871:898	Bacterial cellulose membrane	871:898	Bacterial cellulose membrane was impregnated with the Pistacia atlantica fruit oil.					
34791616	2	108	from	treatment	249:257	arg1	Medicine					326:333	Traditional Persian Medicine	306:333	Traditional Persian Medicine	306:333	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	14	109	theme	bio-safe	2075:2082	arg1	dressing					2084:2091	a potential bio-safe dressing	2063:2091	a potential bio-safe dressing for wound management	2063:2112	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	14	109	theme	bio-safe	2075:2082	arg1	cellulose					2005:2013	bacterial cellulose	1995:2013	bacterial cellulose coated with Pistacia atlantica fruit oil	1995:2054	CONCLUSION The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.					
34791616	3	110	theme	Pistacia	431:438	arg1	oil					456:458	Pistacia atlantica fruit oil	431:458	Pistacia atlantica fruit oil	431:458	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	0	111	theme	cellulose	57:65	arg1	healing					6:12	Wound healing	0:12	Wound healing	0:12	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	0	111	theme	cellulose	57:65	arg1	effects					36:42	anti-inflammatory effects	18:42	anti-inflammatory effects of bacterial cellulose	18:65	Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil.					
34791616	3	112	theme	study	381:385	arg1	objectives					362:371	The main objectives	353:371	The main objectives of this study	353:385	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	8	113	theme	silver	1152:1157	arg1	sulfadiazine					1159:1170	either silver sulfadiazine	1145:1170	either silver sulfadiazine	1145:1170	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	7	114	theme	Pistacia	955:962	arg1	hydrogel					974:981	Pistacia atlantica hydrogel	955:981	Pistacia atlantica hydrogel	955:981	Pistacia atlantica hydrogel was prepared using specific Carbopol.					
34791616	4	115	theme	METHOD	585:590	arg1	membrane					612:619	METHOD Bacterial cellulose membrane	585:619	METHOD Bacterial cellulose membrane	585:619	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	3	116	dep	OBJECTIVES	342:351	arg1	were					387:390	were	387:390	were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model	387:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
34791616	2	117	theme	fruit	208:212	arg1	oil					214:216	The fruit oil	204:216	The fruit oil of the plant	204:229	The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).					
34791616	8	118	contain	containing	1134:1143	arg2	sulfadiazine					1159:1170	either silver sulfadiazine	1145:1170	either silver sulfadiazine	1145:1170	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	8	118	contain	containing	1134:1143	arg1	dressings					1124:1132	the wound dressings	1114:1132	the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil	1114:1296	Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil.					
34791616	4	119	theme	cellulose	602:610	arg1	membrane					612:619	METHOD Bacterial cellulose membrane	585:619	METHOD Bacterial cellulose membrane	585:619	METHOD Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose.					
34791616	13	120	theme	Wound	1822:1826	arg1	closure					1828:1834	Wound closure	1822:1834	Wound closure	1822:1834	Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).					
34791616	10	121	theme	bacterial	1400:1408	arg1	cellulose					1410:1418	the bacterial cellulose	1396:1418	the bacterial cellulose	1396:1418	RESULTS FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum.					
34791616	3	122	theme	anti-inflammatory	491:507	arg1	effects					509:515	anti-inflammatory effects	491:515	anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model	491:582	OBJECTIVES The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.					
32640322	0	0	theme	promising	90:98	arg1	scaffold					100:107	A promising scaffold	88:107	A promising scaffold of biomedical applications	88:134	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	1	1	theme	wound	362:366	arg1	dressing					368:375	wound dressing	362:375	wound dressing	362:375	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	3	2	theme	nanoparticles	550:562	arg1	size					533:536	the average size	521:536	the average size of the TiO2 nanoparticles (NPs)	521:568	The size distribution showed the average size of the TiO2 nanoparticles (NPs) was about 40 nm.					
32640322	1	3	theme	composite	268:276	arg1	hydrogels					278:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels	204:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	204:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	6	4	theme	CH/PPG/TiO2	1097:1107	arg1	hydrogels					1119:1127	the CH/PPG/TiO2 composite hydrogels	1093:1127	the CH/PPG/TiO2 composite hydrogels	1093:1127	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	8	5	from	values	1372:1377	arg1	elongation					1352:1361	elongation	1352:1361	elongation	1352:1361	The tensile strength and elongation at break values of CH/PPG/TiO2 were 3.0 MPa and 31%, respectively.					
32640322	8	5	from	values	1372:1377	arg1	strength					1339:1346	tensile strength	1331:1346	tensile strength	1331:1346	The tensile strength and elongation at break values of CH/PPG/TiO2 were 3.0 MPa and 31%, respectively.					
32640322	1	6	from	preparation	168:178	arg1	view					291:294	view	291:294	view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	291:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	4	7	theme	enzymatic	715:723	arg1	degradation					725:735	in vitro enzymatic degradation	706:735	in vitro enzymatic degradation	706:735	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	10	8	theme	biomedical	1623:1632	arg1	applications					1634:1645	biomedical applications	1623:1645	biomedical applications	1623:1645	Finally, the developed composite scaffold prepared in this study may possess potentially useful in biomedical applications.					
32640322	1	9	theme	hydrogels	278:286	arg1	characterization					184:199	characterization	184:199	characterization	184:199	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	9	theme	hydrogels	278:286	arg1	preparation					168:178	preparation	168:178	preparation	168:178	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	6	10	theme	vapor	1151:1155	arg1	permeability					1157:1168	enhanced water vapor permeability	1136:1168	enhanced water vapor permeability	1136:1168	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	4	11	theme	mechanical	809:818	arg1	tests					820:824	mechanical tests	809:824	mechanical tests	809:824	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	5	12	theme	film	939:942	arg1	activity					905:912	the antimicrobial activity	887:912	the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica	887:1014	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	5	13	theme	diffusion	1044:1052	arg1	method					1054:1059	disc diffusion method	1039:1059	disc diffusion method	1039:1059	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	9	14	theme	composite	1464:1472	arg1	hydrogels					1474:1482	the CH/PPG/TiO2 composite hydrogels	1448:1482	the CH/PPG/TiO2 composite hydrogels	1448:1482	Most importantly, the CH/PPG/TiO2 composite hydrogels showed strong antimicrobial properties.					
32640322	7	15	theme	TiO2	1317:1320	arg1	NPs					1322:1324	TiO2 NPs	1317:1324	TiO2 NPs	1317:1324	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
32640322	1	16	theme	antibacterial	381:393	arg1	materials					395:403	antibacterial materials	381:403	antibacterial materials	381:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	8	17	theme	CH/PPG/TiO2	1382:1392	arg1	values					1372:1377	break values	1366:1377	break values of CH/PPG/TiO2	1366:1392	The tensile strength and elongation at break values of CH/PPG/TiO2 were 3.0 MPa and 31%, respectively.					
32640322	4	18	theme	biomineralization	778:794	arg1	studies					796:802	in vitro biomineralization studies	769:802	in vitro biomineralization studies	769:802	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	7	19	theme	NPs	1322:1324	arg1	addition					1305:1312	the addition	1301:1312	the addition of TiO2 NPs	1301:1324	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
32640322	0	20	theme	applications	123:134	arg1	scaffold					100:107	A promising scaffold	88:107	A promising scaffold of biomedical applications	88:134	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	4	21	theme	contact	667:673	arg1	angle					675:679	water contact angle	661:679	water contact angle	661:679	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	6	22	theme	obtained	1075:1082	arg1	results					1084:1090	the obtained results	1071:1090	the obtained results	1071:1090	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	2	23	theme	CH/PPG/TiO2	419:429	arg1	films					431:435	The prepared CH/PPG/TiO2 films	406:435	The prepared CH/PPG/TiO2 films	406:435	The prepared CH/PPG/TiO2 films were comprehensively characterized by several methods.					
32640322	4	24	theme	water	692:696	arg1	uptake					698:703	water uptake	692:703	water uptake	692:703	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	0	25	theme	biomedical	112:121	arg1	applications					123:134	biomedical applications	112:134	biomedical applications	112:134	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	4	26	theme	swelling	628:635	arg1	ratio					637:641	swelling ratio	628:641	swelling ratio	628:641	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	27	theme	water	644:648	arg1	retention					650:658	water retention	644:658	water retention	644:658	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	6	28	theme	water	1145:1149	arg1	permeability					1157:1168	enhanced water vapor permeability	1136:1168	enhanced water vapor permeability	1136:1168	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	3	29	theme	size	496:499	arg1	distribution					501:512	The size distribution	492:512	The size distribution	492:512	The size distribution showed the average size of the TiO2 nanoparticles (NPs) was about 40 nm.					
32640322	0	30	theme	glycol	23:28	arg1	film					49:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	4	31	theme	other	601:605	arg1	ratio					637:641	swelling ratio	628:641	swelling ratio	628:641	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	angle					675:679	water contact angle	661:679	water contact angle	661:679	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	studies					796:802	in vitro biomineralization studies	769:802	in vitro biomineralization studies	769:802	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	porosity					682:689	porosity	682:689	porosity	682:689	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	properties					607:616	other properties	601:616	other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests	601:824	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	retention					650:658	water retention	644:658	water retention	644:658	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	rate					763:766	water vapor transmission rate	738:766	water vapor transmission rate	738:766	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	degradation					725:735	in vitro enzymatic degradation	706:735	in vitro enzymatic degradation	706:735	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	uptake					698:703	water uptake	692:703	water uptake	692:703	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	31	theme	other	601:605	arg1	tests					820:824	mechanical tests	809:824	mechanical tests	809:824	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	6	32	theme	enhanced	1136:1143	arg1	permeability					1157:1168	enhanced water vapor permeability	1136:1168	enhanced water vapor permeability	1136:1168	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	7	33	theme	thermal	1277:1283	arg1	properties					1285:1294	the examined mechanical and thermal properties	1249:1294	the examined mechanical and thermal properties	1249:1294	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
32640322	1	34	dep	preparation	168:178	arg1	the					164:166	the	164:166	the	164:166	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	35	theme	chitosan/poly	204:216	arg1	hydrogels					278:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels	204:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	204:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	0	36	theme	Chitosan/polypropylene	0:21	arg1	film					49:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	6	37	theme	swelling	1202:1209	arg1	ratio					1211:1215	swelling ratio	1202:1215	swelling ratio	1202:1215	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	2	38	theme	prepared	410:417	arg1	films					431:435	The prepared CH/PPG/TiO2 films	406:435	The prepared CH/PPG/TiO2 films	406:435	The prepared CH/PPG/TiO2 films were comprehensively characterized by several methods.					
32640322	0	39	theme	composite	39:47	arg1	film					49:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	5	40	theme	Candida	997:1003	arg1	lipolytica					1005:1014	Candida lipolytica	997:1014	Candida lipolytica	997:1014	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	1	41	theme	propylene	219:227	arg1	chitosan/poly					204:216	chitosan/poly	204:216	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	204:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	41	theme	propylene	219:227	arg1	glycol					229:234	propylene glycol	219:234	propylene glycol	219:234	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	0	42	theme	hydrogel	30:37	arg1	film					49:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film	0:52	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	1	43	theme	developing	305:314	arg1	dressing					368:375	wound dressing	362:375	wound dressing	362:375	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	43	theme	developing	305:314	arg1	materials					395:403	antibacterial materials	381:403	antibacterial materials	381:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	43	theme	developing	305:314	arg1	applications					316:327	their developing applications	299:327	their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	299:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	43	theme	developing	305:314	arg1	packaging					351:359	antimicrobial packaging	337:359	antimicrobial packaging	337:359	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	44	from	view	291:294	arg1	characterization					184:199	characterization	184:199	characterization	184:199	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	44	from	view	291:294	arg1	preparation					168:178	preparation	168:178	preparation	168:178	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	4	45	theme	in	769:770	arg1	studies					796:802	in vitro biomineralization studies	769:802	in vitro biomineralization studies	769:802	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	3	46	theme	average	525:531	arg1	size					533:536	the average size	521:536	the average size of the TiO2 nanoparticles (NPs)	521:568	The size distribution showed the average size of the TiO2 nanoparticles (NPs) was about 40 nm.					
32640322	1	47	theme	applications	316:327	arg1	view					291:294	view	291:294	view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	291:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	10	48	theme	composite	1547:1555	arg1	scaffold					1557:1564	the developed composite scaffold	1533:1564	the developed composite scaffold prepared in this study	1533:1587	Finally, the developed composite scaffold prepared in this study may possess potentially useful in biomedical applications.					
32640322	6	49	theme	water	1181:1185	arg1	retention					1187:1195	water retention	1181:1195	water retention	1181:1195	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	8	50	theme	break	1366:1370	arg1	values					1372:1377	break values	1366:1377	break values of CH/PPG/TiO2	1366:1392	The tensile strength and elongation at break values of CH/PPG/TiO2 were 3.0 MPa and 31%, respectively.					
32640322	5	51	theme	CH/PPG/TiO2	917:927	arg1	film					939:942	CH/PPG/TiO2 composite film	917:942	CH/PPG/TiO2 composite film	917:942	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	5	52	theme	antimicrobial	891:903	arg1	activity					905:912	the antimicrobial activity	887:912	the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica	887:1014	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	1	53	from	hydrogels	278:286	arg1	view					291:294	view	291:294	view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	291:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	4	54	theme	water	661:665	arg1	angle					675:679	water contact angle	661:679	water contact angle	661:679	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	1	55	theme	/titanium	236:244	arg1	hydrogels					278:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels	204:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	204:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	0	56	theme	TiO2	68:71	arg1	nanoparticles					73:85	TiO2 nanoparticles	68:85	TiO2 nanoparticles	68:85	Chitosan/polypropylene glycol hydrogel composite film designed with TiO2 nanoparticles: A promising scaffold of biomedical applications.					
32640322	10	57	from	useful	1613:1618	arg1	applications					1634:1645	biomedical applications	1623:1645	biomedical applications	1623:1645	Finally, the developed composite scaffold prepared in this study may possess potentially useful in biomedical applications.					
32640322	4	58	theme	water	738:742	arg1	rate					763:766	water vapor transmission rate	738:766	water vapor transmission rate	738:766	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	10	59	theme	developed	1537:1545	arg1	scaffold					1557:1564	the developed composite scaffold	1533:1564	the developed composite scaffold prepared in this study	1533:1587	Finally, the developed composite scaffold prepared in this study may possess potentially useful in biomedical applications.					
32640322	1	60	theme	antimicrobial	337:349	arg1	packaging					351:359	antimicrobial packaging	337:359	antimicrobial packaging	337:359	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	6	61	theme	composite	1109:1117	arg1	hydrogels					1119:1127	the CH/PPG/TiO2 composite hydrogels	1093:1127	the CH/PPG/TiO2 composite hydrogels	1093:1127	Based on the obtained results, the CH/PPG/TiO2 composite hydrogels showed enhanced water vapor permeability, porosity, water retention, and swelling ratio.					
32640322	10	62	from	applications	1634:1645	arg1	useful					1613:1618	useful	1613:1618	useful	1613:1618	Finally, the developed composite scaffold prepared in this study may possess potentially useful in biomedical applications.					
32640322	3	63	theme	TiO2	545:548	arg1	NPs					565:567	NPs	565:567	NPs	565:567	The size distribution showed the average size of the TiO2 nanoparticles (NPs) was about 40 nm.					
32640322	3	63	theme	TiO2	545:548	arg1	nanoparticles					550:562	the TiO2 nanoparticles	541:562	the TiO2 nanoparticles (NPs)	541:568	The size distribution showed the average size of the TiO2 nanoparticles (NPs) was about 40 nm.					
32640322	5	64	theme	disc	1039:1042	arg1	method					1054:1059	disc diffusion method	1039:1059	disc diffusion method	1039:1059	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	7	65	located	observed	1237:1244	arg2	improvement					1221:1231	An improvement	1218:1231	An improvement	1218:1231	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
32640322	7	65	located	observed	1237:1244	arg1	properties					1285:1294	the examined mechanical and thermal properties	1249:1294	the examined mechanical and thermal properties	1249:1294	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
32640322	1	66	theme	present	141:147	arg1	study					149:153	The present study	137:153	The present study	137:153	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	1	67	theme	dioxide	246:252	arg1	hydrogels					278:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels	204:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	204:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	4	68	dep	in	706:707	arg1	vitro					709:713	vitro	709:713	vitro	709:713	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	9	69	theme	CH/PPG/TiO2	1452:1462	arg1	hydrogels					1474:1482	the CH/PPG/TiO2 composite hydrogels	1448:1482	the CH/PPG/TiO2 composite hydrogels	1448:1482	Most importantly, the CH/PPG/TiO2 composite hydrogels showed strong antimicrobial properties.					
32640322	3	70	dep	showed	514:519	arg1	40 nm					580:584	40 nm	580:584	40 nm	580:584	The size distribution showed the average size of the TiO2 nanoparticles (NPs) was about 40 nm.					
32640322	7	71	theme	mechanical	1262:1271	arg1	properties					1285:1294	the examined mechanical and thermal properties	1249:1294	the examined mechanical and thermal properties	1249:1294	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
32640322	4	72	theme	vapor	744:748	arg1	rate					763:766	water vapor transmission rate	738:766	water vapor transmission rate	738:766	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	73	theme	transmission	750:761	arg1	rate					763:766	water vapor transmission rate	738:766	water vapor transmission rate	738:766	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	4	74	dep	in	769:770	arg1	vitro					772:776	vitro	772:776	vitro	772:776	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	8	75	dep	strength	1339:1346	arg1	The					1327:1329	The	1327:1329	The	1327:1329	The tensile strength and elongation at break values of CH/PPG/TiO2 were 3.0 MPa and 31%, respectively.					
32640322	2	76	theme	several	475:481	arg1	methods					483:489	several methods	475:489	several methods	475:489	The prepared CH/PPG/TiO2 films were comprehensively characterized by several methods.					
32640322	5	77	theme	composite	929:937	arg1	film					939:942	CH/PPG/TiO2 composite film	917:942	CH/PPG/TiO2 composite film	917:942	Besides these characterizations, the antimicrobial activity of CH/PPG/TiO2 composite film against Staphylococcus aureus, Escherichia coli, and Candida lipolytica was evaluated by using disc diffusion method.					
32640322	9	78	theme	strong	1491:1496	arg1	properties					1512:1521	strong antimicrobial properties	1491:1521	strong antimicrobial properties	1491:1521	Most importantly, the CH/PPG/TiO2 composite hydrogels showed strong antimicrobial properties.					
32640322	1	79	theme	CH/PPG/TiO2	255:265	arg1	hydrogels					278:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels	204:286	chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	204:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	9	80	theme	antimicrobial	1498:1510	arg1	properties					1512:1521	strong antimicrobial properties	1491:1521	strong antimicrobial properties	1491:1521	Most importantly, the CH/PPG/TiO2 composite hydrogels showed strong antimicrobial properties.					
32640322	1	81	from	characterization	184:199	arg1	view					291:294	view	291:294	view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials	291:403	The present study explores the preparation and characterization of chitosan/poly (propylene glycol)/titanium dioxide (CH/PPG/TiO2) composite hydrogels in view of their developing applications such as antimicrobial packaging, wound dressing and antibacterial materials.					
32640322	4	82	theme	in	706:707	arg1	degradation					725:735	in vitro enzymatic degradation	706:735	in vitro enzymatic degradation	706:735	Additionally, other properties including swelling ratio, water retention, water contact angle, porosity, water uptake, in vitro enzymatic degradation, water vapor transmission rate, in vitro biomineralization studies, and mechanical tests were evaluated in detailed.					
32640322	8	83	theme	tensile	1331:1337	arg1	strength					1339:1346	tensile strength	1331:1346	tensile strength	1331:1346	The tensile strength and elongation at break values of CH/PPG/TiO2 were 3.0 MPa and 31%, respectively.					
32640322	7	84	theme	examined	1253:1260	arg1	properties					1285:1294	the examined mechanical and thermal properties	1249:1294	the examined mechanical and thermal properties	1249:1294	An improvement was observed in the examined mechanical and thermal properties with the addition of TiO2 NPs.					
33916867	3	0	theme	flour	291:295	arg1	application					269:279	the application	265:279	the application of banana flour	265:295	Bread was selected as the product model for the application of banana flour.					
33916867	8	1	theme	different	941:949	arg1	sizes					951:955	different sizes	941:955	different sizes	941:955	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	9	2	dep	greater	1116:1122	arg1	<					1142:1142	p < 0.05	1140:1147	p < 0.05	1140:1147	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	9	3	theme	swelling	1049:1056	arg1	power					1058:1062	swelling power	1049:1062	swelling power	1049:1062	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	11	4	theme	pasting	1317:1323	arg1	viscosity					1375:1383	final viscosity	1369:1383	final viscosity	1369:1383	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	4	theme	pasting	1317:1323	arg1	breakdown					1354:1362	breakdown	1354:1362	breakdown	1354:1362	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	4	theme	pasting	1317:1323	arg1	temperature					1325:1335	a higher pasting temperature	1308:1335	a higher pasting temperature	1308:1335	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	4	theme	pasting	1317:1323	arg1	viscosity					1343:1351	peak viscosity	1338:1351	peak viscosity	1338:1351	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	5	5	theme	WF	600:601	arg1	substitution					604:615	wheat flour (WF) substitution	587:615	wheat flour (WF) substitution at different levels	587:635	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	11	6	theme	rapid	1240:1244	arg1	profile					1274:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	10	7	theme	gelatinization	1155:1168	arg1	ΔH					1180:1181	ΔH	1180:1181	ΔH	1180:1181	The gelatinization enthalpy (ΔH) was 0.69 and 5.00 J/g for WF and UBF, respectively.					
33916867	10	7	theme	gelatinization	1155:1168	arg1	0.69					1188:1191	0.69	1188:1191	0.69	1188:1191	The gelatinization enthalpy (ΔH) was 0.69 and 5.00 J/g for WF and UBF, respectively.					
33916867	10	7	theme	gelatinization	1155:1168	arg1	enthalpy					1170:1177	The gelatinization enthalpy	1151:1177	The gelatinization enthalpy (ΔH)	1151:1182	The gelatinization enthalpy (ΔH) was 0.69 and 5.00 J/g for WF and UBF, respectively.					
33916867	12	8	from	increase	1449:1456	arg1	hardness					1467:1474	bread hardness	1461:1474	bread hardness	1461:1474	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	8	from	increase	1449:1456	arg1	volume					1499:1504	loaf volume	1494:1504	loaf volume (p < 0.05)	1494:1515	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	8	from	increase	1449:1456	arg1	<					1509:1509	p < 0.05	1507:1514	p < 0.05	1507:1514	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	11	9	theme	pasting	1266:1272	arg1	profile					1274:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	4	10	theme	main	365:368	arg1	composition					370:380	the main composition	361:380	the main composition of green bananas	361:397	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	14	11	theme	functional	1637:1646	arg1	ingredient					1653:1662	a functional food ingredient	1635:1662	a functional food ingredient	1635:1662	The utilization of UBF as a functional food ingredient will benefit the consumer.					
33916867	8	12	theme	sizes	951:955	arg1	granules					929:936	Starch granules	922:936	Starch granules of different sizes and shapes (round, long and oblong)	922:991	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	2	13	theme	green	142:146	arg1	bananas					148:154	unripe green bananas	135:154	unripe green bananas obtained from those that were graded as unacceptable for export	135:218	This study aimed to utilize unripe green bananas obtained from those that were graded as unacceptable for export.					
33916867	7	14	theme	fat	866:868	arg1	contents					870:877	protein (15%) and fat contents	848:877	contents	870:877	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	4	15	theme	green	385:389	arg1	bananas					391:397	green bananas	385:397	green bananas	385:397	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	6	16	theme	Quality	657:663	arg1	attributes					665:674	Quality attributes	657:674	Quality attributes of the bread	657:687	Quality attributes of the bread were determined.					
33916867	8	17	theme	Starch	922:927	arg1	granules					929:936	Starch granules	922:936	Starch granules of different sizes and shapes (round, long and oblong)	922:991	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	11	18	theme	peak	1338:1341	arg1	temperature					1325:1335	a higher pasting temperature	1308:1335	a higher pasting temperature	1308:1335	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	18	theme	peak	1338:1341	arg1	viscosity					1343:1351	peak viscosity	1338:1351	peak viscosity	1338:1351	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	2	19	theme	unripe	135:140	arg1	bananas					148:154	unripe green bananas	135:154	unripe green bananas obtained from those that were graded as unacceptable for export	135:218	This study aimed to utilize unripe green bananas obtained from those that were graded as unacceptable for export.					
33916867	7	20	theme	UBF	831:833	arg1	bread					835:839	UBF bread	831:839	UBF bread	831:839	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	5	21	from	properties	501:510	arg1	bread					577:581	bread	577:581	bread	577:581	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	8	22	theme	shapes	961:966	arg1	granules					929:936	Starch granules	922:936	Starch granules of different sizes and shapes (round, long and oblong)	922:991	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	9	23	theme	water	1073:1077	arg1	greater					1116:1122	greater	1116:1122	greater	1116:1122	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	9	23	theme	water	1073:1077	arg1	capacity					1090:1097	the water absorption capacity	1069:1097	the water absorption capacity of WF bread	1069:1109	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	7	24	theme	p	911:911	arg1	bread					904:908	WF bread	901:908	WF bread (p < 0.05)	901:919	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	24	theme	p	911:911	arg1	<					913:913	p < 0.05	911:918	p < 0.05	911:918	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	5	25	theme	UBF	542:544	arg1	properties					501:510	physico-chemical properties	484:510	physico-chemical properties	484:510	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	5	25	theme	UBF	542:544	arg1	application					562:572	its application	558:572	its application in bread for wheat flour (WF) substitution at different levels	558:635	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	5	25	theme	UBF	542:544	arg1	properties					528:537	functional properties	517:537	functional properties	517:537	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	5	25	theme	UBF	542:544	arg1	composition					471:481	The chemical composition	458:481	The chemical composition	458:481	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	14	26	theme	UBF	1628:1630	arg1	utilization					1613:1623	The utilization	1609:1623	The utilization of UBF as a functional food ingredient	1609:1662	The utilization of UBF as a functional food ingredient will benefit the consumer.					
33916867	5	27	theme	chemical	462:469	arg1	composition					471:481	The chemical composition	458:481	The chemical composition	458:481	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	13	28	with	product	1567:1573	arg1	properties					1597:1606	health-promoting properties	1580:1606	health-promoting properties	1580:1606	We show that UBF can be considered a value-added product with health-promoting properties.					
33916867	7	29	located	found	822:826	arg2	activity					808:815	radical scavenging activity	789:815	radical scavenging activity	789:815	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	content					777:783	potassium content	767:783	potassium content	767:783	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	ash					757:759	ash	757:759	ash (2%)	757:764	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg1	bread					835:839	UBF bread	831:839	UBF bread	831:839	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	carbohydrate					711:722	High carbohydrate	706:722	High carbohydrate (89%)	706:728	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	%					727:727	89%	725:727	89%	725:727	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	fiber					745:749	total dietary fiber	731:749	total dietary fiber (7%)	731:754	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	%					753:753	7%	752:753	7%	752:753	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	29	located	found	822:826	arg2	%					763:763	2%	762:763	2%	762:763	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	9	30	theme	absorption	1079:1088	arg1	greater					1116:1122	greater	1116:1122	greater	1116:1122	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	9	30	theme	absorption	1079:1088	arg1	capacity					1090:1097	the water absorption capacity	1069:1097	the water absorption capacity of WF bread	1069:1109	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	5	31	theme	wheat	587:591	arg1	substitution					604:615	wheat flour (WF) substitution	587:615	wheat flour (WF) substitution at different levels	587:635	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	11	32	contain	had	1304:1306	arg2	viscosity					1375:1383	final viscosity	1369:1383	final viscosity	1369:1383	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	32	contain	had	1304:1306	arg2	breakdown					1354:1362	breakdown	1354:1362	breakdown	1354:1362	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	32	contain	had	1304:1306	arg2	temperature					1325:1335	a higher pasting temperature	1308:1335	a higher pasting temperature	1308:1335	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	32	contain	had	1304:1306	arg1	bread					1298:1302	UBF bread	1294:1302	UBF bread	1294:1302	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	32	contain	had	1304:1306	arg2	viscosity					1343:1351	peak viscosity	1338:1351	peak viscosity	1338:1351	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	5	33	theme	different	620:628	arg1	levels					630:635	different levels	620:635	different levels	620:635	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	0	34	theme	Physicochemical	0:14	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties of Bread	0:34	Physicochemical Properties of Bread Partially Substituted with Unripe Green Banana (Cavendish spp.)					
33916867	7	35	theme	total	731:735	arg1	fiber					745:749	total dietary fiber	731:749	total dietary fiber (7%)	731:754	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	35	theme	total	731:735	arg1	%					753:753	7%	752:753	7%	752:753	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	5	36	theme	flour	593:597	arg1	substitution					604:615	wheat flour (WF) substitution	587:615	wheat flour (WF) substitution at different levels	587:635	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	7	37	theme	scavenging	797:806	arg1	activity					808:815	radical scavenging activity	789:815	radical scavenging activity	789:815	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	8	38	dep	shapes	961:966	arg1	long					976:979	long	976:979	long	976:979	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	8	38	dep	shapes	961:966	arg1	round					969:973	round	969:973	round	969:973	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	8	38	dep	shapes	961:966	arg1	oblong					985:990	oblong	985:990	oblong	985:990	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	0	39	theme	Bread	30:34	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties of Bread	0:34	Physicochemical Properties of Bread Partially Substituted with Unripe Green Banana (Cavendish spp.)					
33916867	7	40	theme	dietary	737:743	arg1	fiber					745:749	total dietary fiber	731:749	total dietary fiber (7%)	731:754	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	40	theme	dietary	737:743	arg1	%					753:753	7%	752:753	7%	752:753	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	11	41	theme	RVA	1261:1263	arg1	profile					1274:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	5	42	from	properties	528:537	arg1	bread					577:581	bread	577:581	bread	577:581	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	11	43	theme	higher	1310:1315	arg1	viscosity					1375:1383	final viscosity	1369:1383	final viscosity	1369:1383	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	43	theme	higher	1310:1315	arg1	breakdown					1354:1362	breakdown	1354:1362	breakdown	1354:1362	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	43	theme	higher	1310:1315	arg1	temperature					1325:1335	a higher pasting temperature	1308:1335	a higher pasting temperature	1308:1335	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	43	theme	higher	1310:1315	arg1	viscosity					1343:1351	peak viscosity	1338:1351	peak viscosity	1338:1351	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	9	44	theme	WF	1102:1103	arg1	bread					1105:1109	WF bread	1102:1109	WF bread	1102:1109	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	12	45	theme	bread	1461:1465	arg1	hardness					1467:1474	bread hardness	1461:1474	bread hardness	1461:1474	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	8	46	theme	UBF	1026:1028	arg1	bread					1030:1034	UBF bread	1026:1034	UBF bread	1026:1034	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	13	47	theme	health-promoting	1580:1595	arg1	properties					1597:1606	health-promoting properties	1580:1606	health-promoting properties	1580:1606	We show that UBF can be considered a value-added product with health-promoting properties.					
33916867	9	48	theme	bread	1105:1109	arg1	power					1058:1062	swelling power	1049:1062	swelling power	1049:1062	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	9	48	theme	bread	1105:1109	arg1	greater					1116:1122	greater	1116:1122	greater	1116:1122	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	9	48	theme	bread	1105:1109	arg1	capacity					1090:1097	the water absorption capacity	1069:1097	the water absorption capacity of WF bread	1069:1109	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	9	48	theme	bread	1105:1109	arg1	Solubility					1037:1046	Solubility	1037:1046	Solubility	1037:1046	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	13	49	theme	value-added	1555:1565	arg1	product					1567:1573	a value-added product	1553:1573	a value-added product with health-promoting properties	1553:1606	We show that UBF can be considered a value-added product with health-promoting properties.					
33916867	11	50	theme	viscoanalyzer	1246:1258	arg1	profile					1274:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile	1236:1280	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	51	theme	WF	1390:1391	arg1	bread					1393:1397	WF bread	1390:1397	WF bread (p < 0.05)	1390:1408	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	51	theme	WF	1390:1391	arg1	<					1402:1402	p < 0.05	1400:1407	p < 0.05	1400:1407	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	3	52	theme	product	247:253	arg1	model					255:259	the product model	243:259	the product model for the application of banana flour	243:295	Bread was selected as the product model for the application of banana flour.					
33916867	3	52	theme	product	247:253	arg1	Bread					221:225	Bread	221:225	Bread	221:225	Bread was selected as the product model for the application of banana flour.					
33916867	4	53	theme	other	319:323	arg1	compounds					343:351	other functional active compounds	319:351	other functional active compounds	319:351	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	0	54	theme	Unripe	63:68	arg1	Banana					76:81	Unripe Green Banana	63:81	Unripe Green Banana	63:81	Physicochemical Properties of Bread Partially Substituted with Unripe Green Banana (Cavendish spp.)					
33916867	7	55	theme	WF	901:902	arg1	bread					904:908	WF bread	901:908	WF bread (p < 0.05)	901:919	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	55	theme	WF	901:902	arg1	<					913:913	p < 0.05	911:918	p < 0.05	911:918	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	0	56	dep	Substituted	46:56	arg1	spp					94:96	spp	94:96	spp	94:96	Physicochemical Properties of Bread Partially Substituted with Unripe Green Banana (Cavendish spp.)					
33916867	5	57	theme	functional	517:526	arg1	properties					528:537	functional properties	517:537	functional properties	517:537	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	12	58	theme	p	1507:1507	arg1	volume					1499:1504	loaf volume	1494:1504	loaf volume (p < 0.05)	1494:1515	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	58	theme	p	1507:1507	arg1	<					1509:1509	p < 0.05	1507:1514	p < 0.05	1507:1514	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	59	from	decrease	1482:1489	arg1	hardness					1467:1474	bread hardness	1461:1474	bread hardness	1461:1474	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	59	from	decrease	1482:1489	arg1	volume					1499:1504	loaf volume	1494:1504	loaf volume (p < 0.05)	1494:1515	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	59	from	decrease	1482:1489	arg1	<					1509:1509	p < 0.05	1507:1514	p < 0.05	1507:1514	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	3	60	theme	banana	284:289	arg1	flour					291:295	banana flour	284:295	banana flour	284:295	Bread was selected as the product model for the application of banana flour.					
33916867	11	61	theme	p	1400:1400	arg1	bread					1393:1397	WF bread	1390:1397	WF bread (p < 0.05)	1390:1408	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	61	theme	p	1400:1400	arg1	<					1402:1402	p < 0.05	1400:1407	p < 0.05	1400:1407	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	4	62	theme	active	336:341	arg1	compounds					343:351	other functional active compounds	319:351	other functional active compounds	319:351	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	9	63	theme	UBF	1129:1131	arg1	bread					1133:1137	UBF bread	1129:1137	UBF bread	1129:1137	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	12	64	theme	loaf	1494:1497	arg1	volume					1499:1504	loaf volume	1494:1504	loaf volume (p < 0.05)	1494:1515	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	12	64	theme	loaf	1494:1497	arg1	<					1509:1509	p < 0.05	1507:1514	p < 0.05	1507:1514	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	4	65	theme	bananas	391:397	arg1	composition					370:380	the main composition	361:380	the main composition of green bananas	361:397	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	4	66	theme	functional	325:334	arg1	compounds					343:351	other functional active compounds	319:351	other functional active compounds	319:351	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	7	67	theme	radical	789:795	arg1	activity					808:815	radical scavenging activity	789:815	radical scavenging activity	789:815	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	5	68	from	levels	630:635	arg1	substitution					604:615	wheat flour (WF) substitution	587:615	wheat flour (WF) substitution at different levels	587:635	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	14	69	theme	food	1648:1651	arg1	ingredient					1653:1662	a functional food ingredient	1635:1662	a functional food ingredient	1635:1662	The utilization of UBF as a functional food ingredient will benefit the consumer.					
33916867	4	70	theme	banana	407:412	arg1	UBF					421:423	UBF	421:423	UBF	421:423	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	4	70	theme	banana	407:412	arg1	flour					414:418	unripe banana flour	400:418	unripe banana flour (UBF)	400:424	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	5	71	from	composition	471:481	arg1	bread					577:581	bread	577:581	bread	577:581	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	6	72	theme	bread	683:687	arg1	attributes					665:674	Quality attributes	657:674	Quality attributes of the bread	657:687	Quality attributes of the bread were determined.					
33916867	7	73	theme	potassium	767:775	arg1	content					777:783	potassium content	767:783	potassium content	767:783	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	74	theme	High	706:709	arg1	carbohydrate					711:722	High carbohydrate	706:722	High carbohydrate (89%)	706:728	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	7	74	theme	High	706:709	arg1	%					727:727	89%	725:727	89%	725:727	High carbohydrate (89%), total dietary fiber (7%), ash (2%), potassium content and radical scavenging activity were found in UBF bread, while protein (15%) and fat contents (0.9%) were higher in WF bread (p < 0.05).					
33916867	12	75	theme	UBF	1435:1437	arg1	level					1426:1430	the level	1422:1430	the level of UBF	1422:1437	Increasing the level of UBF caused an increase in bread hardness and a decrease in loaf volume (p < 0.05).					
33916867	4	76	theme	unripe	400:405	arg1	UBF					421:423	UBF	421:423	UBF	421:423	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	4	76	theme	unripe	400:405	arg1	flour					414:418	unripe banana flour	400:418	unripe banana flour (UBF)	400:424	As carbohydrates and other functional active compounds make up the main composition of green bananas, unripe banana flour (UBF) was prepared and characterized.					
33916867	5	77	theme	physico-chemical	484:499	arg1	properties					501:510	physico-chemical properties	484:510	physico-chemical properties	484:510	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	5	78	from	application	562:572	arg1	bread					577:581	bread	577:581	bread	577:581	The chemical composition, physico-chemical properties, and functional properties of UBF, as well as its application in bread for wheat flour (WF) substitution at different levels, were investigated.					
33916867	9	79	theme	p	1140:1140	arg1	<					1142:1142	p < 0.05	1140:1147	p < 0.05	1140:1147	Solubility, swelling power, and the water absorption capacity of WF bread were greater than UBF bread (p < 0.05).					
33916867	0	80	theme	Green	70:74	arg1	Banana					76:81	Unripe Green Banana	63:81	Unripe Green Banana	63:81	Physicochemical Properties of Bread Partially Substituted with Unripe Green Banana (Cavendish spp.)					
33916867	11	81	theme	UBF	1294:1296	arg1	bread					1298:1302	UBF bread	1294:1302	UBF bread	1294:1302	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	8	82	located	observed	998:1005	arg1	starch					1014:1019	the starch	1010:1019	the starch from UBF bread	1010:1034	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	8	82	located	observed	998:1005	arg2	granules					929:936	Starch granules	922:936	Starch granules of different sizes and shapes (round, long and oblong)	922:991	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	8	83	from	bread	1030:1034	arg1	starch					1014:1019	the starch	1010:1019	the starch from UBF bread	1010:1034	Starch granules of different sizes and shapes (round, long and oblong) were observed in the starch from UBF bread.					
33916867	11	84	theme	final	1369:1373	arg1	viscosity					1375:1383	final viscosity	1369:1383	final viscosity	1369:1383	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33916867	11	84	theme	final	1369:1373	arg1	temperature					1325:1335	a higher pasting temperature	1308:1335	a higher pasting temperature	1308:1335	The rapid viscoanalyzer (RVA) pasting profile showed that UBF bread had a higher pasting temperature, peak viscosity, breakdown, and final viscosity than WF bread (p < 0.05).					
33202283	8	0	theme	chain	1100:1104	arg1	measurement					1126:1136	measurement	1126:1136	measurement	1126:1136	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	0	theme	chain	1100:1104	arg1	analysis					1150:1157	PICRUSt analysis	1142:1157	PICRUSt analysis	1142:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	0	theme	chain	1100:1104	arg1	SCFAs					1119:1123	SCFAs	1119:1123	SCFAs	1119:1123	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	0	theme	chain	1100:1104	arg1	acids					1112:1116	Short chain fatty acids	1094:1116	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis	1094:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	9	1	contain	has	1369:1371	arg2	role					1386:1389	a beneficial role	1373:1389	a beneficial role	1373:1389	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	9	1	contain	has	1369:1371	arg1	supplementation					1353:1367	GA supplementation	1350:1367	GA supplementation	1350:1367	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	4	2	theme	w/w	579:581	arg1	%					577:577	0.75% w/w	573:581	0.75% w/w	573:581	CKD was induced by adenine (0.75% w/w) given in the diet daily for four weeks, and GA was administered in drinking water at a concentration of 15% w/v.					
33202283	4	2	theme	w/w	579:581	arg1	adenine					564:570	adenine	564:570	adenine (0.75% w/w) given in the diet daily for four weeks	564:621	CKD was induced by adenine (0.75% w/w) given in the diet daily for four weeks, and GA was administered in drinking water at a concentration of 15% w/v.					
33202283	6	3	theme	Proteobacteria	846:859	arg1	abundance					817:825	The abundance	813:825	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria	813:901	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	0	4	theme	microbiome	60:69	arg1	composition					71:81	the intestinal microbiome composition	45:81	the intestinal microbiome composition in rats with experimental chronic kidney disease	45:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	2	5	from	microbiota	388:397	arg1	rats					402:405	rats	402:405	rats with adenine-induced CKD	402:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	9	6	theme	butyrate	1443:1450	arg1	capacity					1499:1506	its anti-inflammatory, antioxidant capacity	1464:1506	its anti-inflammatory, antioxidant capacity	1464:1506	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	9	6	theme	butyrate	1443:1450	arg1	properties					1529:1538	anti-nitrosative properties	1512:1538	anti-nitrosative properties	1512:1538	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	9	6	theme	butyrate	1443:1450	arg1	production					1429:1438	an increased production	1416:1438	an increased production of butyrate	1416:1450	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	6	7	theme	GA	945:946	arg1	treatment					948:956	GA treatment	945:956	GA treatment	945:956	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	8	8	theme	Short	1094:1098	arg1	measurement					1126:1136	measurement	1126:1136	measurement	1126:1136	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	8	theme	Short	1094:1098	arg1	analysis					1150:1157	PICRUSt analysis	1142:1157	PICRUSt analysis	1142:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	8	theme	Short	1094:1098	arg1	SCFAs					1119:1123	SCFAs	1119:1123	SCFAs	1119:1123	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	8	theme	Short	1094:1098	arg1	acids					1112:1116	Short chain fatty acids	1094:1116	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis	1094:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	7	9	theme	GA	1063:1064	arg1	treatment					1066:1074	GA treatment	1063:1074	GA treatment	1063:1074	Interestingly, alpha and beta diversity index were both reduced with GA treatment in rats with CKD.					
33202283	9	10	theme	anti-nitrosative	1512:1527	arg1	properties					1529:1538	anti-nitrosative properties	1512:1538	anti-nitrosative properties	1512:1538	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	6	11	theme	Actinobacteria	830:843	arg1	abundance					817:825	The abundance	813:825	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria	813:901	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	8	12	theme	GA	1175:1176	arg1	treatment					1178:1186	GA treatment	1175:1186	GA treatment	1175:1186	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	1	13	theme	bidirectional	234:246	arg1	relationship					248:259	a bidirectional relationship	232:259	a bidirectional relationship between microbiota and CKD	232:286	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	0	14	theme	experimental	96:107	arg1	disease					124:130	experimental chronic kidney disease	96:130	experimental chronic kidney disease	96:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	4	15	theme	15	688:689	arg1	%					690:690	%	690:690	%	690:690	CKD was induced by adenine (0.75% w/w) given in the diet daily for four weeks, and GA was administered in drinking water at a concentration of 15% w/v.					
33202283	9	16	dep	anti-inflammatory	1468:1484	arg1	antioxidant					1487:1497	antioxidant	1487:1497	antioxidant	1487:1497	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	3	17	theme	equal	477:481	arg1	adenine					508:514	adenine	508:514	adenine	508:514	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	17	theme	equal	477:481	arg1	control					499:505	control	499:505	control	499:505	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	17	theme	equal	477:481	arg1	n					491:491	n = 6	491:495	n = 6	491:495	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	17	theme	equal	477:481	arg1	groups					483:488	four equal groups	472:488	four equal groups (n = 6): control, adenine, GA and adenine + GA groups	472:542	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	17	theme	equal	477:481	arg1	GA					517:518	GA	517:518	GA	517:518	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	5	18	theme	16s	701:703	arg1	rRNA					705:708	The 16s rRNA	697:708	The 16s rRNA analysis	697:717	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	2	19	theme	gum	354:356	arg1	influence					329:337	the influence	325:337	the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD	325:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	7	20	theme	alpha	1009:1013	arg1	index					1034:1038	alpha and beta diversity index	1009:1038	alpha and beta diversity index	1009:1038	Interestingly, alpha and beta diversity index were both reduced with GA treatment in rats with CKD.					
33202283	2	21	theme	adenine-induced	412:426	arg1	CKD					428:430	adenine-induced CKD	412:430	adenine-induced CKD	412:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	2	22	theme	intestinal	377:386	arg1	microbiota					388:397	the intestinal microbiota	373:397	the intestinal microbiota in rats with adenine-induced CKD	373:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	9	23	theme	beneficial	1375:1384	arg1	role					1386:1389	a beneficial role	1373:1389	a beneficial role	1373:1389	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	8	24	theme	fatty	1106:1110	arg1	measurement					1126:1136	measurement	1126:1136	measurement	1126:1136	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	24	theme	fatty	1106:1110	arg1	analysis					1150:1157	PICRUSt analysis	1142:1157	PICRUSt analysis	1142:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	24	theme	fatty	1106:1110	arg1	SCFAs					1119:1123	SCFAs	1119:1123	SCFAs	1119:1123	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	24	theme	fatty	1106:1110	arg1	acids					1112:1116	Short chain fatty acids	1094:1116	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis	1094:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	4	25	theme	w/v	692:694	arg1	concentration					671:683	a concentration	669:683	a concentration of 15% w/v	669:694	CKD was induced by adenine (0.75% w/w) given in the diet daily for four weeks, and GA was administered in drinking water at a concentration of 15% w/v.					
33202283	8	26	theme	butyrate	1221:1228	arg1	level					1230:1234	the depleted butyrate level	1208:1234	the depleted butyrate level	1208:1234	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	2	27	theme	prebiotic	342:350	arg1	influence					329:337	the influence	325:337	the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD	325:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	0	28	from	influence	4:12	arg1	composition					71:81	the intestinal microbiome composition	45:81	the intestinal microbiome composition in rats with experimental chronic kidney disease	45:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	4	29	theme	%	690:690	arg1	w/v					692:694	15% w/v	688:694	15% w/v	688:694	CKD was induced by adenine (0.75% w/w) given in the diet daily for four weeks, and GA was administered in drinking water at a concentration of 15% w/v.					
33202283	0	30	with	rats	86:89	arg1	disease					124:130	experimental chronic kidney disease	96:130	experimental chronic kidney disease	96:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	8	31	theme	depleted	1212:1219	arg1	level					1230:1234	the depleted butyrate level	1208:1234	the depleted butyrate level	1208:1234	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	5	32	theme	rRNA	705:708	arg1	analysis					710:717	The 16s rRNA analysis	697:717	The 16s rRNA analysis	697:717	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	7	33	theme	diversity	1024:1032	arg1	index					1034:1038	alpha and beta diversity index	1009:1038	alpha and beta diversity index	1009:1038	Interestingly, alpha and beta diversity index were both reduced with GA treatment in rats with CKD.					
33202283	2	34	from	influence	329:337	arg1	microbiota					388:397	the intestinal microbiota	373:397	the intestinal microbiota in rats with adenine-induced CKD	373:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	0	35	theme	kidney	117:122	arg1	disease					124:130	experimental chronic kidney disease	96:130	experimental chronic kidney disease	96:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	3	36	theme	=	493:493	arg1	n					491:491	n = 6	491:495	n = 6	491:495	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	36	theme	=	493:493	arg1	groups					483:488	four equal groups	472:488	four equal groups (n = 6): control, adenine, GA and adenine + GA groups	472:542	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	0	37	theme	chronic	109:115	arg1	disease					124:130	experimental chronic kidney disease	96:130	experimental chronic kidney disease	96:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	0	38	from	composition	71:81	arg1	rats					86:89	rats	86:89	rats with experimental chronic kidney disease	86:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	5	39	theme	microbial	790:798	arg1	composition					800:810	microbial composition	790:810	microbial composition	790:810	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	1	40	theme	common	176:181	arg1	disease					197:203	a globally common and important disease	165:203	a globally common and important disease	165:203	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	1	40	theme	common	176:181	arg1	disease					148:154	Chronic kidney disease	133:154	Chronic kidney disease (CKD)	133:160	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	0	41	theme	gum	31:33	arg1	acacia					35:40	the prebiotic gum acacia	17:40	the prebiotic gum acacia	17:40	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	3	42	dep	groups	483:488	arg1	adenine					508:514	adenine	508:514	adenine	508:514	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	42	dep	groups	483:488	arg1	control					499:505	control	499:505	control	499:505	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	42	dep	groups	483:488	arg1	groups					537:542	adenine + GA groups	524:542	adenine + GA groups	524:542	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	42	dep	groups	483:488	arg1	GA					517:518	GA	517:518	GA	517:518	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	3	42	dep	groups	483:488	arg1	groups					483:488	four equal groups	472:488	four equal groups (n = 6): control, adenine, GA and adenine + GA groups	472:542	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	8	43	theme	CKD	1298:1300	arg1	rats					1302:1305	CKD rats	1298:1305	CKD rats	1298:1305	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	44	theme	perturbated	1248:1258	arg1	pathways					1271:1278	various perturbated functional pathways	1240:1278	various perturbated functional pathways	1240:1278	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	6	45	theme	adenine-induced	920:934	arg1	CKD					936:938	adenine-induced CKD	920:938	adenine-induced CKD	920:938	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	0	46	theme	prebiotic	21:29	arg1	acacia					35:40	the prebiotic gum acacia	17:40	the prebiotic gum acacia	17:40	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	7	47	theme	beta	1019:1022	arg1	index					1034:1038	alpha and beta diversity index	1009:1038	alpha and beta diversity index	1009:1038	Interestingly, alpha and beta diversity index were both reduced with GA treatment in rats with CKD.					
33202283	3	48	theme	GA	534:535	arg1	groups					537:542	adenine + GA groups	524:542	adenine + GA groups	524:542	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	5	49	theme	Miseq	745:749	arg1	region					767:772	Illumina Miseq targeting V3-V4 region	736:772	Illumina Miseq targeting V3-V4 region	736:772	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	1	50	theme	important	187:195	arg1	disease					197:203	a globally common and important disease	165:203	a globally common and important disease	165:203	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	1	50	theme	important	187:195	arg1	disease					148:154	Chronic kidney disease	133:154	Chronic kidney disease (CKD)	133:160	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	5	51	theme	Illumina	736:743	arg1	region					767:772	Illumina Miseq targeting V3-V4 region	736:772	Illumina Miseq targeting V3-V4 region	736:772	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	0	52	theme	acacia	35:40	arg1	influence					4:12	The influence	0:12	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease	0:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	8	53	theme	functional	1260:1269	arg1	pathways					1271:1278	various perturbated functional pathways	1240:1278	various perturbated functional pathways	1240:1278	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	2	54	theme	study	304:308	arg1	aim					293:295	The aim	289:295	The aim of the study	289:308	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	3	55	theme	adenine	524:530	arg1	groups					537:542	adenine + GA groups	524:542	adenine + GA groups	524:542	Animals were randomly distributed into four equal groups (n = 6): control, adenine, GA and adenine + GA groups.					
33202283	2	56	dep	prebiotic	342:350	arg1	acacia					358:363	acacia	358:363	acacia	358:363	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	8	57	theme	various	1240:1246	arg1	pathways					1271:1278	various perturbated functional pathways	1240:1278	various perturbated functional pathways	1240:1278	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	5	58	theme	targeting	751:759	arg1	region					767:772	Illumina Miseq targeting V3-V4 region	736:772	Illumina Miseq targeting V3-V4 region	736:772	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	9	59	theme	increased	1419:1427	arg1	production					1429:1438	an increased production	1416:1438	an increased production of butyrate	1416:1450	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	6	60	theme	Verrucomicrobia	878:892	arg1	abundance					817:825	The abundance	813:825	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria	813:901	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	8	61	dep	acids	1112:1116	arg1	measurement					1126:1136	measurement	1126:1136	measurement	1126:1136	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	61	dep	acids	1112:1116	arg1	analysis					1150:1157	PICRUSt analysis	1142:1157	PICRUSt analysis	1142:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	61	dep	acids	1112:1116	arg1	acids					1112:1116	Short chain fatty acids	1094:1116	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis	1094:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	7	62	with	rats	1079:1082	arg1	CKD					1089:1091	CKD	1089:1091	CKD	1089:1091	Interestingly, alpha and beta diversity index were both reduced with GA treatment in rats with CKD.					
33202283	2	63	with	rats	402:405	arg1	CKD					428:430	adenine-induced CKD	412:430	adenine-induced CKD	412:430	The aim of the study was to examine the influence of prebiotic - gum acacia (GA) on the intestinal microbiota in rats with adenine-induced CKD.					
33202283	5	64	theme	V3-V4	761:765	arg1	region					767:772	Illumina Miseq targeting V3-V4 region	736:772	Illumina Miseq targeting V3-V4 region	736:772	The 16s rRNA analysis was performed on Illumina Miseq targeting V3-V4 region to characterize microbial composition.					
33202283	9	65	theme	GA	1350:1351	arg1	supplementation					1353:1367	GA supplementation	1350:1367	GA supplementation	1350:1367	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	0	66	theme	intestinal	49:58	arg1	composition					71:81	the intestinal microbiome composition	45:81	the intestinal microbiome composition in rats with experimental chronic kidney disease	45:130	The influence of the prebiotic gum acacia on the intestinal microbiome composition in rats with experimental chronic kidney disease.					
33202283	1	67	theme	Chronic	133:139	arg1	CKD					157:159	CKD	157:159	CKD	157:159	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	1	67	theme	Chronic	133:139	arg1	disease					148:154	Chronic kidney disease	133:154	Chronic kidney disease (CKD)	133:160	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	1	67	theme	Chronic	133:139	arg1	disease					197:203	a globally common and important disease	165:203	a globally common and important disease	165:203	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	8	68	theme	PICRUSt	1142:1148	arg1	analysis					1150:1157	PICRUSt analysis	1142:1157	PICRUSt analysis	1142:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	8	68	theme	PICRUSt	1142:1148	arg1	acids					1112:1116	Short chain fatty acids	1094:1116	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis	1094:1157	Short chain fatty acids (SCFAs) measurement and PICRUSt analysis have shown that GA treatment completely restored the depleted butyrate level and various perturbated functional pathways, respectively, in CKD rats.					
33202283	6	69	theme	Tenericutes	862:872	arg1	abundance					817:825	The abundance	813:825	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria	813:901	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	6	70	dep	Actinobacteria	830:843	arg1	bacteria					894:901	bacteria	894:901	bacteria	894:901	The abundance of Actinobacteria, Proteobacteria, Tenericutes and Verrucomicrobia bacteria was increased in adenine-induced CKD, and GA treatment successfully reversed those levels.					
33202283	9	71	theme	anti-inflammatory	1468:1484	arg1	capacity					1499:1506	its anti-inflammatory, antioxidant capacity	1464:1506	its anti-inflammatory, antioxidant capacity	1464:1506	Taking together, our results suggest that GA supplementation has a beneficial role in treating CKD, through an increased production of butyrate, as well as its anti-inflammatory, antioxidant capacity and anti-nitrosative properties.					
33202283	1	72	theme	kidney	141:146	arg1	CKD					157:159	CKD	157:159	CKD	157:159	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	1	72	theme	kidney	141:146	arg1	disease					148:154	Chronic kidney disease	133:154	Chronic kidney disease (CKD)	133:160	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
33202283	1	72	theme	kidney	141:146	arg1	disease					197:203	a globally common and important disease	165:203	a globally common and important disease	165:203	Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and CKD.					
34814709	3	0	theme	particle	832:839	arg1	sizes					841:845	superfine particle sizes	822:845	superfine particle sizes (i.e. 50-100 μm and 100-180 μm)	822:877	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	2	1	theme	fine	655:658	arg1	powders					660:666	the fine powders	651:666	the fine powders	651:666	Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses.					
34814709	0	2	theme	differential	123:134	arg1	process					144:150	controlled differential sieving process	112:150	controlled differential sieving process from four medicinal plants	112:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	3	3	theme	HE	995:996	arg1	extracts					998:1005	HE extracts	995:1005	HE extracts	995:1005	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	4	theme	more	985:988	arg1	%					983:983	at least ca. 20%	968:983	at least ca. 20% more than HE extracts (all, p<0.05)	968:1019	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	5	theme	LPS-stimulated	286:299	arg1	PBMCs					345:349	PBMCs	345:349	PBMCs	345:349	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	5	theme	LPS-stimulated	286:299	arg1	cells					338:342	LPS-stimulated primary peripheral blood mononuclear cells	286:342	LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18)	286:367	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	0	6	theme	controlled	112:121	arg1	process					144:150	controlled differential sieving process	112:150	controlled differential sieving process from four medicinal plants	112:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	5	7	theme	bioactive	1274:1282	arg1	compounds					1284:1292	bioactive compounds	1274:1292	bioactive compounds as well as their biological activities	1274:1331	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	1	8	theme	primary	301:307	arg1	PBMCs					345:349	PBMCs	345:349	PBMCs	345:349	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	8	theme	primary	301:307	arg1	cells					338:342	LPS-stimulated primary peripheral blood mononuclear cells	286:342	LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18)	286:367	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	0	9	from	concentrations	64:77	arg1	powders					92:98	superfine powders	82:98	superfine powders obtained by controlled differential sieving process from four medicinal plants	82:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	2	10	theme	phytochemical	622:634	arg1	composition					636:646	phytochemical composition	622:646	phytochemical composition of the fine powders and HE extracts	622:682	Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses.					
34814709	3	11	dep	μm	860:861	arg1	i.e.					848:851	i.e.	848:851	i.e.	848:851	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	12	theme	Rosa	208:211	arg1	effect					198:203	Anti-inflammatory effect	180:203	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix	180:268	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	0	13	theme	sieving	136:142	arg1	process					144:150	controlled differential sieving process	112:150	controlled differential sieving process from four medicinal plants	112:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	1	14	theme	homogeneous	382:392	arg1	powders					394:400	homogeneous powders	382:400	homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction	382:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	2	15	theme	extracts	675:682	arg1	composition					636:646	phytochemical composition	622:646	phytochemical composition of the fine powders and HE extracts	622:682	Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses.					
34814709	1	16	theme	total	546:550	arg1	materials					558:566	total plant materials	546:566	total plant materials obtained via hydroethanolic (HE) extraction	546:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	0	17	theme	anti-inflammatory	10:26	arg1	activity					28:35	anti-inflammatory activity	10:35	anti-inflammatory activity	10:35	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	3	18	from	production	929:938	arg1	PBMCs					958:962	LPS-stimulated PBMCs	943:962	LPS-stimulated PBMCs	943:962	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	2	19	theme	HE	672:673	arg1	extracts					675:682	HE extracts	672:682	HE extracts	672:682	Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses.					
34814709	5	20	theme	environmentally	1206:1220	arg1	CDSp					1194:1197	CDSp	1194:1197	CDSp	1194:1197	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	5	20	theme	environmentally	1206:1220	arg1	method					1231:1236	an environmentally friendly method	1203:1236	an environmentally friendly method	1203:1236	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	1	21	theme	plant	552:556	arg1	materials					558:566	total plant materials	546:566	total plant materials obtained via hydroethanolic (HE) extraction	546:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	22	dep	microparticles	411:424	arg1	microparticles					411:424	small microparticles	405:424	small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction	405:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	22	dep	microparticles	411:424	arg1	μm					446:447	50-100 μm, 100-180 μm and 180-315 μm	427:462	μm	446:447	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	22	dep	microparticles	411:424	arg1	μm					461:462	50-100 μm, 100-180 μm and 180-315 μm	427:462	μm	461:462	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	23	theme	peripheral	309:318	arg1	PBMCs					345:349	PBMCs	345:349	PBMCs	345:349	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	23	theme	peripheral	309:318	arg1	cells					338:342	LPS-stimulated primary peripheral blood mononuclear cells	286:342	LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18)	286:367	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	3	24	theme	one-hour	748:755	arg1	pretreatment					757:768	a one-hour pretreatment	746:768	a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm),	746:878	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	5	25	theme	friendly	1222:1229	arg1	CDSp					1194:1197	CDSp	1194:1197	CDSp	1194:1197	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	5	25	theme	friendly	1222:1229	arg1	method					1231:1236	an environmentally friendly method	1203:1236	an environmentally friendly method	1203:1236	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	1	26	theme	Salix	221:225	arg1	effect					198:203	Anti-inflammatory effect	180:203	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix	180:268	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	27	theme	blood	320:324	arg1	PBMCs					345:349	PBMCs	345:349	PBMCs	345:349	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	27	theme	blood	320:324	arg1	cells					338:342	LPS-stimulated primary peripheral blood mononuclear cells	286:342	LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18)	286:367	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	2	28	theme	powders	660:666	arg1	composition					636:646	phytochemical composition	622:646	phytochemical composition of the fine powders and HE extracts	622:682	Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses.					
34814709	3	29	theme	superfine	822:830	arg1	sizes					841:845	superfine particle sizes	822:845	superfine particle sizes (i.e. 50-100 μm and 100-180 μm)	822:877	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	5	30	theme	compounds	1284:1292	arg1	concentration					1257:1269	the concentration	1253:1269	the concentration of bioactive compounds as well as their biological activities	1253:1331	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	1	31	theme	mononuclear	326:336	arg1	PBMCs					345:349	PBMCs	345:349	PBMCs	345:349	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	31	theme	mononuclear	326:336	arg1	cells					338:342	LPS-stimulated primary peripheral blood mononuclear cells	286:342	LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18)	286:367	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	5	32	theme	biological	1311:1320	arg1	activities					1322:1331	their biological activities	1305:1331	bioactive compounds as well as their biological activities	1274:1331	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
34814709	0	33	theme	phytochemical	50:62	arg1	concentrations					64:77	phytochemical concentrations	50:77	phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants	50:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	4	34	theme	HE	1144:1145	arg1	extracts					1147:1154	their HE extracts	1138:1154	their HE extracts	1138:1154	For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts.					
34814709	1	35	theme	small	405:409	arg1	microparticles					411:424	small microparticles	405:424	small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction	405:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	35	theme	small	405:409	arg1	μm					446:447	50-100 μm, 100-180 μm and 180-315 μm	427:462	μm	446:447	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	35	theme	small	405:409	arg1	μm					461:462	50-100 μm, 100-180 μm and 180-315 μm	427:462	μm	461:462	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	4	36	from	contents	1124:1131	arg1	concentrated					1099:1110	concentrated	1099:1110	concentrated	1099:1110	For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts.					
34814709	1	37	theme	hydroethanolic	581:594	arg1	extraction					601:610	hydroethanolic (HE) extraction	581:610	hydroethanolic (HE) extraction	581:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	37	theme	hydroethanolic	581:594	arg1	HE					597:598	HE	597:598	HE	597:598	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	38	theme	Scrophularia	233:244	arg1	nodosa					246:251	Scrophularia nodosa	233:251	Scrophularia nodosa	233:251	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	0	39	theme	medicinal	162:170	arg1	plants					172:177	four medicinal plants	157:177	four medicinal plants	157:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	1	40	theme	microparticles	411:424	arg1	powders					394:400	homogeneous powders	382:400	homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction	382:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	41	dep	Salix	221:225	arg1	alba					227:230	Salix alba	221:230	Salix alba	221:230	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	2	42	theme	LC-PDA-ESI/MS	702:714	arg1	analyses					716:723	LC-PDA-ESI/MS analyses	702:723	LC-PDA-ESI/MS analyses	702:723	Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses.					
34814709	1	43	theme	nodosa	246:251	arg1	effect					198:203	Anti-inflammatory effect	180:203	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix	180:268	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	44	from	mice	357:360	arg1	PBMCs					345:349	PBMCs	345:349	PBMCs	345:349	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	44	from	mice	357:360	arg1	cells					338:342	LPS-stimulated primary peripheral blood mononuclear cells	286:342	LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18)	286:367	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	3	45	theme	fine	784:787	arg1	powders					789:795	fine powders	784:795	fine powders	784:795	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	45	theme	fine	784:787	arg1	those					811:815	those	811:815	those	811:815	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	46	from	IL-1β	910:914	arg1	PBMCs					958:962	LPS-stimulated PBMCs	943:962	LPS-stimulated PBMCs	943:962	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	47	dep	Rosa	208:211	arg1	helix					264:268	helix	264:268	helix	264:268	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	47	dep	Rosa	208:211	arg1	canina					213:218	Rosa canina	208:218	Rosa canina	208:218	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	4	48	theme	phenolic	1115:1122	arg1	contents					1124:1131	phenolic contents	1115:1131	phenolic contents	1115:1131	For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts.					
34814709	4	49	theme	superfine	1060:1068	arg1	fractions					1079:1087	their superfine powdered fractions	1054:1087	their superfine powdered fractions	1054:1087	For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts.					
34814709	4	50	from	concentrated	1099:1110	arg1	contents					1124:1131	phenolic contents	1115:1131	phenolic contents	1115:1131	For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts.					
34814709	3	51	from	IL-6	917:920	arg1	PBMCs					958:962	LPS-stimulated PBMCs	943:962	LPS-stimulated PBMCs	943:962	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	52	theme	controlled	492:501	arg1	process					524:530	a controlled differential sieving process	490:530	a controlled differential sieving process (CDSp)	490:537	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	52	theme	controlled	492:501	arg1	CDSp					533:536	CDSp	533:536	CDSp	533:536	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	3	53	theme	PBMCs	773:777	arg1	pretreatment					757:768	a one-hour pretreatment	746:768	a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm),	746:878	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	54	theme	μm	434:435	arg1	microparticles					411:424	small microparticles	405:424	small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction	405:610	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	54	theme	μm	434:435	arg1	μm					446:447	50-100 μm, 100-180 μm and 180-315 μm	427:462	μm	446:447	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	55	theme	differential	503:514	arg1	process					524:530	a controlled differential sieving process	490:530	a controlled differential sieving process (CDSp)	490:537	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	55	theme	differential	503:514	arg1	CDSp					533:536	CDSp	533:536	CDSp	533:536	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	0	56	from	plants	172:177	arg1	process					144:150	controlled differential sieving process	112:150	controlled differential sieving process from four medicinal plants	112:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	3	57	theme	NO	926:927	arg1	production					929:938	NO production	926:938	NO production	926:938	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	58	theme	sieving	516:522	arg1	process					524:530	a controlled differential sieving process	490:530	a controlled differential sieving process (CDSp)	490:537	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	1	58	theme	sieving	516:522	arg1	CDSp					533:536	CDSp	533:536	CDSp	533:536	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	3	59	with	pretreatment	757:768	arg1	powders					789:795	fine powders	784:795	fine powders	784:795	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	59	with	pretreatment	757:768	arg1	those					811:815	those	811:815	those	811:815	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	60	theme	Hedera	257:262	arg1	effect					198:203	Anti-inflammatory effect	180:203	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix	180:268	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	3	61	dep	sizes	841:845	arg1	μm					860:861	50-100 μm	853:861	50-100 μm	853:861	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	61	dep	sizes	841:845	arg1	μm					875:876	100-180 μm	867:876	100-180 μm	867:876	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	4	62	theme	powdered	1070:1077	arg1	fractions					1079:1087	their superfine powdered fractions	1054:1087	their superfine powdered fractions	1054:1087	For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts.					
34814709	3	63	dep	20	981:982	arg1	ca.					977:979	ca.	977:979	ca.	977:979	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	0	64	theme	superfine	82:90	arg1	powders					92:98	superfine powders	82:98	superfine powders obtained by controlled differential sieving process from four medicinal plants	82:177	Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants.					
34814709	3	65	from	TNFα	904:907	arg1	PBMCs					958:962	LPS-stimulated PBMCs	943:962	LPS-stimulated PBMCs	943:962	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	66	theme	LPS-stimulated	943:956	arg1	PBMCs					958:962	LPS-stimulated PBMCs	943:962	LPS-stimulated PBMCs	943:962	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	1	67	theme	Anti-inflammatory	180:196	arg1	effect					198:203	Anti-inflammatory effect	180:203	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix	180:268	Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100-180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction.					
34814709	3	68	dep	%	983:983	arg1	all					1008:1010	all	1008:1010	all	1008:1010	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	3	68	dep	%	983:983	arg1	p<0.05					1013:1018	p<0.05	1013:1018	p<0.05	1013:1018	Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50-100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05).					
34814709	5	69	theme	activities	1322:1331	arg1	concentration					1257:1269	the concentration	1253:1269	the concentration of bioactive compounds as well as their biological activities	1253:1331	Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.					
32665560	0	0	theme	phosphate-releasing	78:96	arg1	constructs					152:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	2	1	theme	grafts	509:514	arg1	use					470:472	the use	466:472	the use of 'gold standard' autologous bone grafts	466:514	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	1	2	theme	multifaceted	190:201	arg1	process					230:236	a multifaceted, coordinated physiological process	188:236	a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state	188:347	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	1	2	theme	multifaceted	190:201	arg1	repair					178:183	Bone fracture repair	164:183	Bone fracture repair	164:183	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	9	3	theme	callus	1460:1465	arg1	formation					1467:1475	increased callus formation	1450:1475	increased callus formation	1450:1475	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
32665560	0	4	theme	self-contained	63:76	arg1	constructs					152:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	9	5	contain	carrying	1516:1523	arg2	hydroxyapatite					1530:1543	hydroxyapatite	1530:1543	hydroxyapatite	1530:1543	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
32665560	9	5	contain	carrying	1516:1523	arg2	pyrophosphatase					1549:1563	pyrophosphatase	1549:1563	pyrophosphatase	1549:1563	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
32665560	9	5	contain	carrying	1516:1523	arg1	scaffold					1507:1514	the scaffold	1503:1514	the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone	1503:1676	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
32665560	3	6	theme	such	526:529	arg1	grafts					531:536	such grafts	526:536	such grafts	526:536	However, such grafts may not be readily available, and procedural complications may result in undesired outcomes.					
32665560	10	7	theme	fracture	1805:1812	arg1	healing					1814:1820	bone fracture healing	1800:1820	bone fracture healing	1800:1820	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	0	8	theme	purine-crosslinked	109:126	arg1	constructs					152:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	6	9	with	embedded	1014:1021	arg1	cells					1028:1032	cells	1028:1032	cells	1028:1032	First, scaffolds embedded with cells were characterized for cell morphology, viability, and attachment.					
32665560	10	10	contain	has	1761:1763	arg2	capacity					1777:1784	a promising capacity	1765:1784	a promising capacity to facilitate bone fracture healing	1765:1820	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	10	10	contain	has	1761:1763	arg1	scaffold					1752:1759	the pyrophosphatase-hydroxyapatite composite scaffold	1707:1759	the pyrophosphatase-hydroxyapatite composite scaffold	1707:1759	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	8	11	theme	fracture	1421:1428	arg1	model					1380:1384	a mouse model	1372:1384	a mouse model of intramedullary rod-fixed tibial fracture	1372:1428	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	1	12	theme	fractured	312:320	arg1	bone					322:325	the fractured bone	308:325	the fractured bone to its original state	308:347	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	0	13	theme	injectable	98:107	arg1	constructs					152:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	8	14	theme	mouse	1374:1378	arg1	model					1380:1384	a mouse model	1372:1384	a mouse model of intramedullary rod-fixed tibial fracture	1372:1428	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	6	15	theme	embedded	1014:1021	arg1	scaffolds					1004:1012	scaffolds	1004:1012	scaffolds embedded with cells	1004:1032	First, scaffolds embedded with cells were characterized for cell morphology, viability, and attachment.					
32665560	0	16	theme	constructs	152:161	arg1	properties					49:58	osteogenic properties	38:58	osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	38:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	5	17	theme	scaffolds	876:884	arg1	potential					817:825	the osteogenic potential	802:825	the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite	802:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	4	18	theme	bone	762:765	arg1	grafts					767:772	bone grafts	762:772	bone grafts	762:772	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	8	19	theme	intramedullary	1389:1402	arg1	fracture					1421:1428	intramedullary rod-fixed tibial fracture	1389:1428	intramedullary rod-fixed tibial fracture	1389:1428	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	2	20	theme	defective	435:443	arg1	bones					445:449	defective bones	435:449	defective bones	435:449	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	0	21	theme	chitosan-hydroxyapatite	128:150	arg1	constructs					152:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	5	22	theme	pyrophosphatase	944:958	arg1	activity					960:967	pyrophosphatase activity	944:967	pyrophosphatase activity	944:967	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	10	23	theme	pyrophosphatase-hydroxyapatite	1711:1740	arg1	scaffold					1752:1759	the pyrophosphatase-hydroxyapatite composite scaffold	1707:1759	the pyrophosphatase-hydroxyapatite composite scaffold	1707:1759	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	10	24	theme	bone	1800:1803	arg1	healing					1814:1820	bone fracture healing	1800:1820	bone fracture healing	1800:1820	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	1	25	theme	physiological	216:228	arg1	process					230:236	a multifaceted, coordinated physiological process	188:236	a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state	188:347	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	1	25	theme	physiological	216:228	arg1	repair					178:183	Bone fracture repair	164:183	Bone fracture repair	164:183	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	7	26	theme	phosphatase	1213:1223	arg1	threefold					1186:1194	threefold	1186:1194	threefold	1186:1194	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	7	26	theme	phosphatase	1213:1223	arg1	activity					1225:1232	double alkaline phosphatase activity	1197:1232	double alkaline phosphatase activity	1197:1232	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	2	27	theme	bone	504:507	arg1	grafts					509:514	'gold standard' autologous bone grafts	477:514	'gold standard' autologous bone grafts	477:514	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	7	28	dep	threefold	1186:1194	arg1	to					1183:1184	to	1183:1184	to	1183:1184	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	1	29	dep	multifaceted	190:201	arg1	coordinated					204:214	coordinated	204:214	coordinated	204:214	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	5	30	theme	hydroxyapatite	912:925	arg1	incorporation					895:907	the incorporation	891:907	the incorporation of hydroxyapatite	891:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	0	31	theme	In	0:1	arg1	investigation					21:33	In vitro and in vivo investigation	0:33	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.	0:162	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	8	32	theme	rod-fixed	1404:1412	arg1	fracture					1421:1428	intramedullary rod-fixed tibial fracture	1389:1428	intramedullary rod-fixed tibial fracture	1389:1428	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	4	33	theme	potentials	688:697	arg1	ease					647:650	the ease	643:650	the ease of use and tremendous customization potentials	643:697	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	0	34	theme	in	13:14	arg1	investigation					21:33	In vitro and in vivo investigation	0:33	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.	0:162	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	9	35	theme	fracture	1484:1491	arg1	site					1493:1496	the fracture site	1480:1496	the fracture site	1480:1496	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
32665560	5	36	theme	chitosan	867:874	arg1	scaffolds					876:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds	830:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite	830:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	5	37	theme	5'-diphosphate-crosslinked	840:865	arg1	scaffolds					876:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds	830:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite	830:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	1	38	theme	original	334:341	arg1	state					343:347	its original state	330:347	its original state	330:347	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	7	39	theme	cell-laden	1105:1114	arg1	scaffolds					1116:1124	The cell-laden scaffolds	1101:1124	The cell-laden scaffolds	1101:1124	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	4	40	theme	grafts	767:772	arg1	alternative					747:757	a more suitable alternative	731:757	a more suitable alternative of bone grafts	731:772	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	1	41	theme	new	252:254	arg1	formation					261:269	new bone formation	252:269	new bone formation	252:269	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	2	42	theme	autologous	493:502	arg1	grafts					509:514	'gold standard' autologous bone grafts	477:514	'gold standard' autologous bone grafts	477:514	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	2	43	theme	different	374:382	arg1	approaches					384:393	different approaches	374:393	different approaches	374:393	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	10	44	theme	composite	1742:1750	arg1	scaffold					1752:1759	the pyrophosphatase-hydroxyapatite composite scaffold	1707:1759	the pyrophosphatase-hydroxyapatite composite scaffold	1707:1759	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	1	45	theme	bone	256:259	arg1	formation					261:269	new bone formation	252:269	new bone formation	252:269	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	4	46	theme	customization	674:686	arg1	potentials					688:697	tremendous customization potentials	663:697	tremendous customization potentials	663:697	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	5	47	with	scaffolds	876:884	arg1	incorporation					895:907	the incorporation	891:907	the incorporation of hydroxyapatite	891:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	8	48	theme	fracture	1355:1362	arg1	site					1364:1367	the fracture site	1351:1367	the fracture site	1351:1367	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	3	49	theme	undesired	611:619	arg1	outcomes					621:628	undesired outcomes	611:628	undesired outcomes	611:628	However, such grafts may not be readily available, and procedural complications may result in undesired outcomes.					
32665560	2	50	theme	bones	445:449	arg1	repair					425:430	the repair	421:430	the repair of defective bones	421:449	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	0	51	theme	osteogenic	38:47	arg1	properties					49:58	osteogenic properties	38:58	osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	38:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	9	52	theme	control	1586:1592	arg1	scaffolds					1594:1602	the control scaffolds	1582:1602	the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone	1582:1676	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
32665560	8	53	theme	tibial	1414:1419	arg1	fracture					1421:1428	intramedullary rod-fixed tibial fracture	1389:1428	intramedullary rod-fixed tibial fracture	1389:1428	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	2	54	theme	approaches	384:393	arg1	variety					363:369	a variety	361:369	a variety of different approaches	361:393	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	2	54	theme	approaches	384:393	arg1	approaches					384:393	different approaches	374:393	different approaches	374:393	Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold standard' autologous bone grafts.					
32665560	5	55	theme	osteogenic	806:815	arg1	potential					817:825	the osteogenic potential	802:825	the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite	802:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	7	56	theme	osterix	1238:1244	arg1	expression					1246:1255	osterix expression	1238:1255	osterix expression	1238:1255	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	7	56	theme	osterix	1238:1244	arg1	threefold					1186:1194	threefold	1186:1194	threefold	1186:1194	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	5	57	theme	guanosine	830:838	arg1	scaffolds					876:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds	830:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite	830:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	7	58	theme	alkaline	1204:1211	arg1	phosphatase					1213:1223	double alkaline phosphatase	1197:1223	double alkaline phosphatase activity	1197:1232	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	1	59	theme	Bone	164:167	arg1	process					230:236	a multifaceted, coordinated physiological process	188:236	a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state	188:347	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	1	59	theme	Bone	164:167	arg1	repair					178:183	Bone fracture repair	164:183	Bone fracture repair	164:183	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	3	60	theme	procedural	572:581	arg1	complications					583:595	procedural complications	572:595	procedural complications	572:595	However, such grafts may not be readily available, and procedural complications may result in undesired outcomes.					
32665560	0	61	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	1	62	theme	fracture	169:176	arg1	process					230:236	a multifaceted, coordinated physiological process	188:236	a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state	188:347	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	1	62	theme	fracture	169:176	arg1	repair					178:183	Bone fracture repair	164:183	Bone fracture repair	164:183	Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state.					
32665560	8	63	theme	chitosan	1314:1321	arg1	scaffolds					1323:1331	chitosan scaffolds	1314:1331	chitosan scaffolds	1314:1331	Next, chitosan scaffolds were implanted at the fracture site in a mouse model of intramedullary rod-fixed tibial fracture.					
32665560	5	64	link	5'-diphosphate-crosslinked	840:865	arg1	scaffolds					876:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds	830:884	guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite	830:925	In this study, we examined the osteogenic potential of guanosine 5'-diphosphate-crosslinked chitosan scaffolds with the incorporation of hydroxyapatite, with or without pyrophosphatase activity, both in vitro and in vivo.					
32665560	10	65	theme	promising	1767:1775	arg1	capacity					1777:1784	a promising capacity	1765:1784	a promising capacity to facilitate bone fracture healing	1765:1820	These results indicate that the pyrophosphatase-hydroxyapatite composite scaffold has a promising capacity to facilitate bone fracture healing.					
32665560	4	66	theme	suitable	738:745	arg1	alternative					747:757	a more suitable alternative	731:757	a more suitable alternative of bone grafts	731:772	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	7	67	theme	double	1197:1202	arg1	phosphatase					1213:1223	double alkaline phosphatase	1197:1223	double alkaline phosphatase activity	1197:1232	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	6	68	theme	cell	1057:1060	arg1	morphology					1062:1071	cell morphology	1057:1071	cell morphology	1057:1071	First, scaffolds embedded with cells were characterized for cell morphology, viability, and attachment.					
32665560	7	69	theme	calcium	1271:1277	arg1	phosphate					1279:1287	calcium phosphate	1271:1287	calcium phosphate	1271:1287	The cell-laden scaffolds were found to significantly enhance proliferation for up to threefold, double alkaline phosphatase activity and osterix expression, and increase calcium phosphate deposits in vitro.					
32665560	4	70	theme	use	655:657	arg1	ease					647:650	the ease	643:650	the ease of use and tremendous customization potentials	643:697	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	4	71	theme	tremendous	663:672	arg1	potentials					688:697	tremendous customization potentials	663:697	tremendous customization potentials	663:697	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	0	72	theme	properties	49:58	arg1	investigation					21:33	In vitro and in vivo investigation	0:33	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.	0:162	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	0	73	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	0	74	link	purine-crosslinked	109:126	arg1	constructs					152:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs	63:161	In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs.					
32665560	4	75	theme	synthetic	700:708	arg1	materials					710:718	synthetic materials	700:718	synthetic materials	700:718	Considering the ease of use and tremendous customization potentials, synthetic materials may become a more suitable alternative of bone grafts.					
32665560	9	76	theme	increased	1450:1458	arg1	formation					1467:1475	increased callus formation	1450:1475	increased callus formation	1450:1475	Our results showed increased callus formation at the fracture site with the scaffold carrying both hydroxyapatite and pyrophosphatase in comparison to the control scaffolds lacking both pyrophosphatase and hydroxyapatite, or pyrophosphatase alone.					
31920175	7	0	theme	TPU/CNF-PDA	1506:1516	arg1	nanofibers					1528:1537	TPU/CNF-PDA composite nanofibers	1506:1537	TPU/CNF-PDA composite nanofibers	1506:1537	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	6	1	theme	bonds	1331:1335	arg1	formation					1302:1310	the formation	1298:1310	the formation of strong hydrogen bonds between PDA and TPU/CNF	1298:1359	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	4	2	theme	active	923:928	arg1	sites					930:934	the active sites	919:934	the active sites	919:934	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	9	3	theme	general	1843:1849	arg1	route					1851:1855	a general route	1841:1855	a general route to modify the surface of scaffolds, which are promising for tissue engineering applications	1841:1947	The presented strategy represents a general route to modify the surface of scaffolds, which are promising for tissue engineering applications.					
31920175	3	4	with	nanofibers	655:664	arg1	structure					682:690	core/shell structure	671:690	core/shell structure	671:690	Subsequently, the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure were fabricated by PDA coating method.					
31920175	4	5	theme	CNF	900:902	arg1	addition					888:895	the addition	884:895	the addition of CNF	884:902	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	3	6	theme	core/shell	671:680	arg1	structure					682:690	core/shell structure	671:690	core/shell structure	671:690	Subsequently, the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure were fabricated by PDA coating method.					
31920175	6	7	dep	making	1362:1367	arg1	adhere					1395:1400	adhere	1395:1400	strongly adhere to each other	1386:1414	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	1	8	theme	appropriate	264:274	arg1	property					293:300	the appropriate surface chemical property	260:300	the appropriate surface chemical property	260:300	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	1	8	theme	appropriate	264:274	arg1	important					310:318	important	310:318	important	310:318	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	6	9	theme	strong	1315:1320	arg1	bonds					1331:1335	strong hydrogen bonds	1315:1335	strong hydrogen bonds between PDA and TPU/CNF	1315:1359	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	4	10	theme	layer	800:804	arg1	uniformization					770:783	the uniformization	766:783	the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers	766:851	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	1	11	theme	surface	276:282	arg1	property					293:300	the appropriate surface chemical property	260:300	the appropriate surface chemical property	260:300	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	1	11	theme	surface	276:282	arg1	important					310:318	important	310:318	important	310:318	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	0	12	theme	cellulose	104:112	arg1	nanofibrils					114:124	cellulose nanofibrils	104:124	cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers	104:201	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	0	12	theme	cellulose	104:112	arg1	CNF					127:129	CNF	127:129	CNF	127:129	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	4	13	with	comparison	734:743	arg1	nanofibers					754:763	TPU nanofibers	750:763	TPU nanofibers	750:763	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	7	14	theme	embryonic	1455:1463	arg1	osteoblasts					1465:1475	mouse embryonic osteoblasts	1449:1475	mouse embryonic osteoblasts	1449:1475	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	8	15	theme	excellent	1685:1693	arg1	properties					1721:1730	excellent mechanical and biological properties	1685:1730	excellent mechanical and biological properties	1685:1730	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	4	16	theme	coating	792:798	arg1	layer					800:804	PDA coating layer	788:804	PDA coating layer	788:804	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	1	17	theme	chemical	284:291	arg1	property					293:300	the appropriate surface chemical property	260:300	the appropriate surface chemical property	260:300	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	1	17	theme	chemical	284:291	arg1	important					310:318	important	310:318	important	310:318	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	2	18	theme	CNF	532:534	arg1	particles					537:545	cellulose nanofibrils (CNF) particles	509:545	cellulose nanofibrils (CNF) particles	509:545	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	5	19	theme	TPU	1140:1142	arg1	nanofibers					1156:1165	TPU and TPU/CNF nanofibers	1140:1165	TPU and TPU/CNF nanofibers	1140:1165	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	8	20	contain	have	1680:1683	arg1	nanofibers					1669:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	8	20	contain	have	1680:1683	arg2	properties					1721:1730	excellent mechanical and biological properties	1685:1730	excellent mechanical and biological properties	1685:1730	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	6	21	theme	nanofibers	1223:1232	arg1	properties					1183:1192	The mechanical properties	1168:1192	The mechanical properties of the TPU/CNF-PDA composite nanofibers	1168:1232	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	6	21	theme	nanofibers	1223:1232	arg1	higher					1239:1244	higher	1239:1244	higher	1239:1244	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	7	22	theme	TPU/CNF	1585:1591	arg1	nanofibers					1593:1602	TPU/CNF nanofibers	1585:1602	TPU/CNF nanofibers	1585:1602	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	7	23	theme	osteoblasts	1465:1475	arg1	attachment					1421:1430	attachment	1421:1430	attachment	1421:1430	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	7	23	theme	osteoblasts	1465:1475	arg1	viability					1436:1444	viability	1436:1444	viability of mouse embryonic osteoblasts	1436:1475	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	4	24	theme	composite	832:840	arg1	nanofibers					842:851	TPU/CNF composite nanofibers	824:851	TPU/CNF composite nanofibers	824:851	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	6	25	theme	composite	1213:1221	arg1	nanofibers					1223:1232	the TPU/CNF-PDA composite nanofibers	1197:1232	the TPU/CNF-PDA composite nanofibers	1197:1232	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	4	26	theme	PDA	949:951	arg1	particles					953:961	the PDA particles	945:961	the PDA particles accumulated along with the fiber direction	945:1004	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	7	27	theme	attachment	1421:1430	arg1	MC3T3-E1					1484:1491	MC3T3-E1	1484:1491	MC3T3-E1	1484:1491	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	7	27	theme	attachment	1421:1430	arg1	cells					1477:1481	The attachment and viability of mouse embryonic osteoblasts cells	1417:1481	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers	1417:1537	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	0	28	theme	composite	11:19	arg1	scaffolds					21:29	Biomimetic composite scaffolds	0:29	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication	0:94	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	5	29	theme	TPU/CNF	1148:1154	arg1	nanofibers					1156:1165	TPU and TPU/CNF nanofibers	1140:1165	TPU and TPU/CNF nanofibers	1140:1165	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	4	30	theme	fiber	990:994	arg1	direction					996:1004	the fiber direction	986:1004	the fiber direction	986:1004	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	6	31	theme	TPU/CNF-PDA	1201:1211	arg1	nanofibers					1223:1232	the TPU/CNF-PDA composite nanofibers	1197:1232	the TPU/CNF-PDA composite nanofibers	1197:1232	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	8	32	theme	tissue	1777:1782	arg1	scaffolds					1796:1804	tissue engineering scaffolds	1777:1804	tissue engineering scaffolds	1777:1804	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	2	33	theme	TPU	401:403	arg1	nanofibers					406:415	the thermoplastic polyurethane (TPU) nanofibers	369:415	the thermoplastic polyurethane (TPU) nanofibers	369:415	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	0	34	theme	Biomimetic	0:9	arg1	scaffolds					21:29	Biomimetic composite scaffolds	0:29	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication	0:94	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	0	35	mod	modification	48:59	arg1	polydopamine					64:75	polydopamine	64:75	polydopamine	64:75	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	0	35	mod	modification	48:59	arg3	surface					40:46	surface modification	40:59	surface modification of polydopamine	40:75	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	6	36	theme	due	1291:1293	arg1	nanofibers					1280:1289	the TPU and TPU/CNF nanofibers	1260:1289	nanofibers	1280:1289	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	4	37	from	uniformization	770:783	arg1	surface					813:819	the surface	809:819	the surface of TPU/CNF composite nanofibers	809:851	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	6	38	theme	hydrogen	1322:1329	arg1	bonds					1331:1335	strong hydrogen bonds	1315:1335	strong hydrogen bonds between PDA and TPU/CNF	1315:1359	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	9	39	theme	scaffolds	1882:1890	arg1	surface					1871:1877	the surface	1867:1877	the surface of scaffolds, which are promising for tissue engineering applications	1867:1947	The presented strategy represents a general route to modify the surface of scaffolds, which are promising for tissue engineering applications.					
31920175	4	40	used	used	911:914	arg2	which					905:909	which	905:909	which	905:909	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	6	41	theme	TPU	1264:1266	arg1	nanofibers					1280:1289	the TPU and TPU/CNF nanofibers	1260:1289	nanofibers	1280:1289	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	4	42	theme	PDA	788:790	arg1	layer					800:804	PDA coating layer	788:804	PDA coating layer	788:804	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	5	43	dep	absorption	1017:1026	arg1	The					1007:1009	The	1007:1009	The	1007:1009	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	7	44	theme	viability	1436:1444	arg1	MC3T3-E1					1484:1491	MC3T3-E1	1484:1491	MC3T3-E1	1484:1491	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	7	44	theme	viability	1436:1444	arg1	cells					1477:1481	The attachment and viability of mouse embryonic osteoblasts cells	1417:1481	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers	1417:1537	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	0	45	theme	surface	40:46	arg1	modification					48:59	surface modification	40:59	surface modification of polydopamine	40:75	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	0	46	theme	electrospun	147:157	arg1	nanofibers					192:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	3	47	theme	TPU/CNF-polydopamine	618:637	arg1	nanofibers					655:664	the TPU/CNF-polydopamine (PDA) composite nanofibers	614:664	the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure	614:690	Subsequently, the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure were fabricated by PDA coating method.					
31920175	5	48	with	comparison	1115:1124	arg1	those					1131:1135	those	1131:1135	those	1131:1135	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	9	49	theme	tissue	1917:1922	arg1	applications					1936:1947	tissue engineering applications	1917:1947	tissue engineering applications	1917:1947	The presented strategy represents a general route to modify the surface of scaffolds, which are promising for tissue engineering applications.					
31920175	4	50	theme	nanofibers	842:851	arg1	surface					813:819	the surface	809:819	the surface of TPU/CNF composite nanofibers	809:851	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	9	51	theme	presented	1811:1819	arg1	strategy					1821:1828	The presented strategy	1807:1828	The presented strategy	1807:1828	The presented strategy represents a general route to modify the surface of scaffolds, which are promising for tissue engineering applications.					
31920175	1	52	theme	tissue	324:329	arg1	scaffolds					343:351	tissue engineering scaffolds	324:351	tissue engineering scaffolds	324:351	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	0	53	theme	polyurethane	173:184	arg1	nanofibers					192:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	0	54	theme	polydopamine	64:75	arg1	modification					48:59	surface modification	40:59	surface modification of polydopamine	40:75	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	5	55	theme	TPU/CNF-PDA	1050:1060	arg1	nanofibers					1072:1081	TPU/CNF-PDA composite nanofibers	1050:1081	TPU/CNF-PDA composite nanofibers	1050:1081	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	4	56	theme	TPU	750:752	arg1	nanofibers					754:763	TPU nanofibers	750:763	TPU nanofibers	750:763	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	1	57	theme	engineering	331:341	arg1	scaffolds					343:351	tissue engineering scaffolds	324:351	tissue engineering scaffolds	324:351	To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds.					
31920175	0	58	theme	thermoplastic	159:171	arg1	nanofibers					192:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	5	59	theme	nanofibers	1072:1081	arg1	absorption					1017:1026	water absorption	1011:1026	water absorption	1011:1026	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	5	59	theme	nanofibers	1072:1081	arg1	hydrophilicity					1032:1045	hydrophilicity	1032:1045	hydrophilicity	1032:1045	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	5	60	theme	composite	1062:1070	arg1	nanofibers					1072:1081	TPU/CNF-PDA composite nanofibers	1050:1081	TPU/CNF-PDA composite nanofibers	1050:1081	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	8	61	theme	modified	1638:1645	arg1	nanofibers					1669:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	8	62	theme	biological	1710:1719	arg1	properties					1721:1730	excellent mechanical and biological properties	1685:1730	excellent mechanical and biological properties	1685:1730	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	7	63	theme	mouse	1449:1453	arg1	osteoblasts					1465:1475	mouse embryonic osteoblasts	1449:1475	mouse embryonic osteoblasts	1449:1475	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	0	64	theme	TPU	187:189	arg1	nanofibers					192:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	electrospun thermoplastic polyurethane (TPU) nanofibers	147:201	Biomimetic composite scaffolds based on surface modification of polydopamine on ultrasonication induced cellulose nanofibrils (CNF) adsorbing onto electrospun thermoplastic polyurethane (TPU) nanofibers.					
31920175	3	65	theme	composite	645:653	arg1	nanofibers					655:664	the TPU/CNF-polydopamine (PDA) composite nanofibers	614:664	the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure	614:690	Subsequently, the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure were fabricated by PDA coating method.					
31920175	6	66	theme	TPU/CNF	1272:1278	arg1	nanofibers					1280:1289	the TPU and TPU/CNF nanofibers	1260:1289	nanofibers	1280:1289	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	9	67	theme	engineering	1924:1934	arg1	applications					1936:1947	tissue engineering applications	1917:1947	tissue engineering applications	1917:1947	The presented strategy represents a general route to modify the surface of scaffolds, which are promising for tissue engineering applications.					
31920175	2	68	mod	modified	495:502	arg3	particles					537:545	cellulose nanofibrils (CNF) particles	509:545	cellulose nanofibrils (CNF) particles	509:545	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	2	68	mod	modified	495:502	arg3	ultrasonic-assisted					550:568	ultrasonic-assisted	550:568	ultrasonic-assisted	550:568	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	2	68	mod	modified	495:502	arg1	surface					483:489	its surface	479:489	its surface	479:489	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	2	69	theme	TPU/CNF	580:586	arg1	nanofibers					588:597	TPU/CNF nanofibers	580:597	TPU/CNF nanofibers	580:597	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	7	70	theme	composite	1518:1526	arg1	nanofibers					1528:1537	TPU/CNF-PDA composite nanofibers	1506:1537	TPU/CNF-PDA composite nanofibers	1506:1537	The attachment and viability of mouse embryonic osteoblasts cells (MC3T3-E1) cultured on TPU/CNF-PDA composite nanofibers were obviously enhanced compared with TPU and TPU/CNF nanofibers.					
31920175	5	71	theme	water	1011:1015	arg1	absorption					1017:1026	water absorption	1011:1026	water absorption	1011:1026	The water absorption and hydrophilicity of TPU/CNF-PDA composite nanofibers were significantly increased in comparison with those of TPU and TPU/CNF nanofibers.					
31920175	6	72	theme	mechanical	1172:1181	arg1	properties					1183:1192	The mechanical properties	1168:1192	The mechanical properties of the TPU/CNF-PDA composite nanofibers	1168:1232	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	6	72	theme	mechanical	1172:1181	arg1	higher					1239:1244	higher	1239:1244	higher	1239:1244	The mechanical properties of the TPU/CNF-PDA composite nanofibers were higher than those of the TPU and TPU/CNF nanofibers due to the formation of strong hydrogen bonds between PDA and TPU/CNF, making TPU, CNF and PDA strongly adhere to each other.					
31920175	8	73	theme	engineering	1784:1794	arg1	scaffolds					1796:1804	tissue engineering scaffolds	1777:1804	tissue engineering scaffolds	1777:1804	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	2	74	theme	nanofibrils	519:529	arg1	particles					537:545	cellulose nanofibrils (CNF) particles	509:545	cellulose nanofibrils (CNF) particles	509:545	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	3	75	theme	PDA	640:642	arg1	nanofibers					655:664	the TPU/CNF-polydopamine (PDA) composite nanofibers	614:664	the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure	614:690	Subsequently, the TPU/CNF-polydopamine (PDA) composite nanofibers with core/shell structure were fabricated by PDA coating method.					
31920175	2	76	theme	polyurethane	387:398	arg1	nanofibers					406:415	the thermoplastic polyurethane (TPU) nanofibers	369:415	the thermoplastic polyurethane (TPU) nanofibers	369:415	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	8	77	theme	composite	1659:1667	arg1	nanofibers					1669:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	2	78	theme	cellulose	509:517	arg1	particles					537:545	cellulose nanofibrils (CNF) particles	509:545	cellulose nanofibrils (CNF) particles	509:545	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	4	79	theme	TPU/CNF	824:830	arg1	nanofibers					842:851	TPU/CNF composite nanofibers	824:851	TPU/CNF composite nanofibers	824:851	In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/CNF composite nanofibers significantly increased due to the addition of CNF, which used as the active sites to guide the PDA particles accumulated along with the fiber direction.					
31920175	2	80	theme	thermoplastic	373:385	arg1	nanofibers					406:415	the thermoplastic polyurethane (TPU) nanofibers	369:415	the thermoplastic polyurethane (TPU) nanofibers	369:415	In this study, the thermoplastic polyurethane (TPU) nanofibers was firstly fabricated by electrospinning technique, and then its surface was modified with cellulose nanofibrils (CNF) particles by ultrasonic-assisted to obtain TPU/CNF nanofibers.					
31920175	8	81	theme	mechanical	1695:1704	arg1	properties					1721:1730	excellent mechanical and biological properties	1685:1730	excellent mechanical and biological properties	1685:1730	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
31920175	8	82	theme	TPU/CNF-PDA	1647:1657	arg1	nanofibers					1669:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	the modified TPU/CNF-PDA composite nanofibers	1634:1678	Those results suggested that the modified TPU/CNF-PDA composite nanofibers have excellent mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.					
32443193	1	0	from	present	451:457	arg1	food					471:474	ingested food	462:474	ingested food	462:474	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	1	0	from	present	451:457	arg1	organs					489:494	internal organs	480:494	internal organs	480:494	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	0	1	theme	European	165:172	arg1	beetle					185:190	the largest European saproxylic beetle	153:190	the largest European saproxylic beetle	153:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	0	2	from	beetle	185:190	arg1	correlates					97:106	correlates	97:106	correlates	97:106	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	0	2	from	beetle	185:190	arg1	Patterns					84:91	Patterns	84:91	Patterns	84:91	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	2	3	from	fractions	641:649	arg1	degree					606:611	degree	606:611	degree	606:611	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	3	from	fractions	641:649	arg1	metals					614:619	metals	614:619	metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens)	614:736	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	7	4	theme	fat	2059:2061	arg1	quantities					2031:2040	the large quantities	2021:2040	the large quantities of residual body fat	2021:2061	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	5	5	from	habitat	1553:1559	arg1	individuals					1512:1522	Smaller individuals	1504:1522	Smaller individuals from both woodland and urban habitat	1504:1559	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	1	6	theme	ingested	462:469	arg1	food					471:474	ingested food	462:474	ingested food	462:474	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	2	7	theme	present	717:723	arg1	organs					692:697	body organs	687:697	body organs with gut material present in abdomens	687:735	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	5	8	dep	elements	1607:1614	arg1	Pb					1629:1630	Pb	1629:1630	Pb	1629:1630	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	8	dep	elements	1607:1614	arg1	Zn					1617:1618	Zn	1617:1618	Zn	1617:1618	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	8	dep	elements	1607:1614	arg1	As					1621:1622	As	1621:1622	As	1621:1622	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	8	dep	elements	1607:1614	arg1	Ni					1636:1637	Ni	1636:1637	Ni	1636:1637	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	8	dep	elements	1607:1614	arg1	elements					1607:1614	trace elements	1601:1614	trace elements (Zn, As, Cd, Pb and Ni)	1601:1638	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	8	dep	elements	1607:1614	arg1	Cd					1625:1626	Cd	1625:1626	Cd	1625:1626	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	2	9	theme	Stag	837:840	arg1	Beetle					842:847	the Stag Beetle Lucanus cervus	833:862	the Stag Beetle Lucanus cervus	833:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	9	theme	Stag	837:840	arg1	insect					825:830	the largest European saproxylic insect	793:830	the largest European saproxylic insect	793:830	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	1	10	theme	Stoichiometric	193:206	arg1	data					238:241	Stoichiometric, trophic and ecotoxicological data	193:241	Stoichiometric, trophic and ecotoxicological data	193:241	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	3	11	theme	continent-wide	973:986	arg1	variation					988:996	the continent-wide variation	969:996	the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	969:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	6	12	dep	elements	1671:1678	arg1	Mn					1692:1693	Mn	1692:1693	Mn	1692:1693	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	12	dep	elements	1671:1678	arg1	Cd					1696:1697	Cd	1696:1697	Cd	1696:1697	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	12	dep	elements	1671:1678	arg1	K					1685:1685	K	1685:1685	K	1685:1685	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	12	dep	elements	1671:1678	arg1	Na					1688:1689	Na	1688:1689	Na	1688:1689	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	12	dep	elements	1671:1678	arg1	Ni					1703:1704	Ni	1703:1704	Ni	1703:1704	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	12	dep	elements	1671:1678	arg1	elements					1671:1678	only six elements	1662:1678	only six elements (Mg, K, Na, Mn, Cd and Ni)	1662:1705	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	12	dep	elements	1671:1678	arg1	Mg					1681:1682	Mg	1681:1682	Mg	1681:1682	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	2	13	theme	gut	704:706	arg1	material					708:715	gut material	704:715	gut material	704:715	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	6	14	theme	elements	1671:1678	arg1	concentration					1645:1657	The concentration	1641:1657	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni)	1641:1705	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	1	15	from	traits	316:321	arg1	patterns					281:288	patterns	281:288	patterns of variation in elemental traits of whole invertebrate bodies	281:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	1	16	theme	trophic	209:215	arg1	data					238:241	Stoichiometric, trophic and ecotoxicological data	193:241	Stoichiometric, trophic and ecotoxicological data	193:241	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	2	17	theme	crucial	555:561	arg1	question					564:571	an unexplored, yet crucial, question	536:571	an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions	536:954	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	0	18	theme	intraspecific	126:138	arg1	variation					140:148	continent-wide intraspecific variation	111:148	continent-wide intraspecific variation in the largest European saproxylic beetle	111:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	5	19	theme	elements	1607:1614	arg1	concentrations					1583:1596	higher concentrations	1576:1596	higher concentrations of trace elements (Zn, As, Cd, Pb and Ni)	1576:1638	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	1	20	theme	ecotoxicological	221:236	arg1	data					238:241	Stoichiometric, trophic and ecotoxicological data	193:241	Stoichiometric, trophic and ecotoxicological data	193:241	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	3	21	from	elytra	1111:1116	arg1	populations					1151:1161	28 populations	1148:1161	28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1148:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	2	22	theme	local	931:935	arg1	conditions					945:954	local habitat conditions	931:954	local habitat conditions	931:954	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	0	23	theme	largest	157:163	arg1	beetle					185:190	the largest European saproxylic beetle	153:190	the largest European saproxylic beetle	153:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	3	24	from	abdomen	1122:1128	arg1	populations					1151:1161	28 populations	1148:1161	28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1148:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	7	25	theme	residual	2045:2052	arg1	fat					2059:2061	residual body fat	2045:2061	residual body fat	2045:2061	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	3	26	theme	specimens	1133:1141	arg1	abdomen					1122:1128	abdomen	1122:1128	abdomen	1122:1128	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	26	theme	specimens	1133:1141	arg1	elytra					1111:1116	the elytra	1107:1116	the elytra	1107:1116	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	0	27	theme	saproxylic	174:183	arg1	beetle					185:190	the largest European saproxylic beetle	153:190	the largest European saproxylic beetle	153:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	4	28	theme	differences	1430:1440	arg1	related					1446:1452	related	1446:1452	related	1446:1452	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	4	28	theme	differences	1430:1440	arg1	magnitude					1411:1419	the magnitude	1407:1419	the magnitude of these differences	1407:1440	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	3	29	theme	urban-woodland	1177:1190	arg1	gradient					1200:1207	an urban-woodland habitat gradient	1174:1207	an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1174:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	0	30	theme	elemental	66:74	arg1	traits					76:81	chitin-based and internal elemental traits	40:81	chitin-based and internal elemental traits	40:81	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	6	31	theme	broader	1818:1824	arg1	applicability					1826:1838	the broader applicability	1814:1838	the broader applicability of elytra	1814:1848	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	2	32	theme	major	630:634	arg1	fractions					641:649	two major body fractions	626:649	two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens)	626:736	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	33	from	populations	778:788	arg1	other					764:768	other	764:768	other	764:768	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	4	34	theme	habitat	1484:1490	arg1	conditions					1492:1501	local habitat conditions	1478:1501	local habitat conditions	1478:1501	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	2	35	from	other	764:768	arg1	populations					778:788	wild populations	773:788	wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus	773:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	5	36	theme	Smaller	1504:1510	arg1	individuals					1512:1522	Smaller individuals	1504:1522	Smaller individuals from both woodland and urban habitat	1504:1559	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	1	37	theme	origin	401:406	arg1	issue					374:378	the critical issue	361:378	the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs,	361:495	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	3	38	theme	elements	1035:1042	arg1	concentrations					1005:1018	the concentrations	1001:1018	the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1001:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	39	dep	Spain	1262:1266	arg1	to					1268:1269	to	1268:1269	to	1268:1269	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	39	dep	Spain	1262:1266	arg1	Russia					1271:1276	Russia	1271:1276	Russia	1271:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	40	theme	distributional	1236:1249	arg1	range					1251:1255	' entire distributional range	1227:1255	the species' entire distributional range from Spain to Russia	1216:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	0	41	theme	Breaking	0:7	arg1	stoichiometry					21:33	Breaking down insect stoichiometry	0:33	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.	0:191	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	1	42	theme	elemental	306:314	arg1	traits					316:321	elemental traits	306:321	elemental traits of whole invertebrate bodies	306:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	7	43	theme	saproxylic	1934:1943	arg1	beetles					1945:1951	non-feeding adult saproxylic beetles	1916:1951	non-feeding adult saproxylic beetles	1916:1951	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	0	44	theme	insect	14:19	arg1	stoichiometry					21:33	Breaking down insect stoichiometry	0:33	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.	0:191	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	6	45	theme	internal	1869:1876	arg1	pools					1888:1892	internal elemental pools	1869:1892	internal elemental pools	1869:1892	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	2	46	dep	internal	677:684	arg1	organs					692:697	body organs	687:697	body organs with gut material present in abdomens	687:735	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	7	47	theme	large	2025:2029	arg1	quantities					2031:2040	the large quantities	2021:2040	the large quantities of residual body fat	2021:2061	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	7	48	theme	non-feeding	1916:1926	arg1	beetles					1945:1951	non-feeding adult saproxylic beetles	1916:1951	non-feeding adult saproxylic beetles	1916:1951	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	1	49	theme	invertebrate	332:343	arg1	bodies					345:350	whole invertebrate bodies	326:350	whole invertebrate bodies	326:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	5	50	contain	have	1571:1574	arg1	individuals					1512:1522	Smaller individuals	1504:1522	Smaller individuals from both woodland and urban habitat	1504:1559	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	50	contain	have	1571:1574	arg2	concentrations					1583:1596	higher concentrations	1576:1596	higher concentrations of trace elements (Zn, As, Cd, Pb and Ni)	1576:1638	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	4	51	theme	abdominal	1369:1377	arg1	samples					1379:1385	abdominal samples	1369:1385	abdominal samples	1369:1385	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	3	52	dep	elements	1035:1042	arg1	K					1053:1053	K	1053:1053	K	1053:1053	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Cd					1080:1081	Cd	1080:1081	Cd	1080:1081	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Na					1056:1057	Na	1056:1057	Na	1056:1057	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Zn					1068:1069	Zn	1068:1069	Zn	1068:1069	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Ca					1045:1046	Ca	1045:1046	Ca	1045:1046	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Cu					1072:1073	Cu	1072:1073	Cu	1072:1073	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Pb					1084:1085	Pb	1084:1085	Pb	1084:1085	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Mg					1049:1050	Mg	1049:1050	Mg	1049:1050	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Mn					1060:1061	Mn	1060:1061	Mn	1060:1061	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Ni					1091:1092	Ni	1091:1092	Ni	1091:1092	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	Fe					1064:1065	Fe	1064:1065	Fe	1064:1065	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	52	dep	elements	1035:1042	arg1	elements					1035:1042	12 chemical elements	1023:1042	12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1023:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	6	53	theme	population	1770:1779	arg1	levels					1781:1786	the individual and population levels	1751:1786	levels	1781:1786	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	3	54	dep	species	1220:1226	arg1	range					1251:1255	' entire distributional range	1227:1255	the species' entire distributional range from Spain to Russia	1216:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	6	55	from	levels	1781:1786	arg1	abdomen					1740:1746	abdomen	1740:1746	abdomen	1740:1746	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	6	55	from	levels	1781:1786	arg1	elytra					1729:1734	elytra	1729:1734	elytra	1729:1734	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	2	56	theme	European	805:812	arg1	Beetle					842:847	the Stag Beetle Lucanus cervus	833:862	the Stag Beetle Lucanus cervus	833:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	56	theme	European	805:812	arg1	insect					825:830	the largest European saproxylic insect	793:830	the largest European saproxylic insect	793:830	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	5	57	theme	woodland	1534:1541	arg1	habitat					1553:1559	both woodland and urban habitat	1529:1559	both woodland and urban habitat	1529:1559	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	1	58	from	food	471:474	arg1	present					451:457	present	451:457	present	451:457	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	5	59	theme	urban	1547:1551	arg1	habitat					1553:1559	both woodland and urban habitat	1529:1559	both woodland and urban habitat	1529:1559	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	2	60	theme	wild	773:776	arg1	populations					778:788	wild populations	773:788	wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus	773:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	1	61	theme	variation	293:301	arg1	patterns					281:288	patterns	281:288	patterns of variation in elemental traits of whole invertebrate bodies	281:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	2	62	theme	insect	825:830	arg1	populations					778:788	wild populations	773:788	wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus	773:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	63	dep	Beetle	842:847	arg1	cervus					857:862	the Stag Beetle Lucanus cervus	833:862	the Stag Beetle Lucanus cervus	833:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	64	with	organs	692:697	arg1	material					708:715	gut material	704:715	gut material	704:715	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	1	65	theme	internal	480:487	arg1	organs					489:494	internal organs	480:494	internal organs	480:494	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	0	66	theme	variation	140:148	arg1	correlates					97:106	correlates	97:106	correlates	97:106	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	0	66	theme	variation	140:148	arg1	Patterns					84:91	Patterns	84:91	Patterns	84:91	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	6	67	theme	individual	1755:1764	arg1	levels					1781:1786	the individual and population levels	1751:1786	levels	1781:1786	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	5	68	theme	trace	1601:1605	arg1	Ni					1636:1637	Ni	1636:1637	Ni	1636:1637	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	68	theme	trace	1601:1605	arg1	Zn					1617:1618	Zn	1617:1618	Zn	1617:1618	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	68	theme	trace	1601:1605	arg1	elements					1607:1614	trace elements	1601:1614	trace elements (Zn, As, Cd, Pb and Ni)	1601:1638	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	68	theme	trace	1601:1605	arg1	Cd					1625:1626	Cd	1625:1626	Cd	1625:1626	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	5	68	theme	trace	1601:1605	arg1	Pb					1629:1630	Pb	1629:1630	Pb	1629:1630	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	3	69	from	Spain	1262:1266	arg1	range					1251:1255	' entire distributional range	1227:1255	the species' entire distributional range from Spain to Russia	1216:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	1	70	dep	acquired	267:274	arg1	whereas					353:359	whereas	353:359	whereas	353:359	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	1	71	from	patterns	281:288	arg1	traits					316:321	elemental traits	306:321	elemental traits of whole invertebrate bodies	306:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	1	72	theme	portion	416:422	arg1	origin					401:406	the extracellular origin	383:406	the extracellular origin of some portion of elements, such as those present in ingested food and internal organs,	383:495	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	4	73	theme	insect	1462:1467	arg1	size					1469:1472	insect size	1462:1472	insect size	1462:1472	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	2	74	dep	whether	585:591	arg1	correlated					743:752	correlated	743:752	are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus	739:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	3	75	from	variation	988:996	arg1	concentrations					1005:1018	the concentrations	1001:1018	the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1001:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	2	76	theme	unexplored	539:548	arg1	question					564:571	an unexplored, yet crucial, question	536:571	an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions	536:954	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	77	theme	habitat	937:943	arg1	conditions					945:954	local habitat conditions	931:954	local habitat conditions	931:954	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	78	attach	present	717:723	arg2	organs					692:697	body organs	687:697	body organs with gut material present in abdomens	687:735	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	78	attach	present	717:723	arg1	abdomens					728:735	abdomens	728:735	abdomens	728:735	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	1	79	theme	elements	427:434	arg1	portion					416:422	some portion	411:422	some portion of elements, such as those present in ingested food and internal organs,	411:495	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	1	80	from	variation	293:301	arg1	traits					316:321	elemental traits	306:321	elemental traits of whole invertebrate bodies	306:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	2	81	dep	fractions	641:649	arg1	elytra					665:670	elytra	665:670	elytra	665:670	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	81	dep	fractions	641:649	arg1	internal					677:684	internal	677:684	internal	677:684	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	81	dep	fractions	641:649	arg1	exoskeleton					652:662	exoskeleton	652:662	exoskeleton (elytra)	652:671	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	0	82	from	correlates	97:106	arg1	beetle					185:190	the largest European saproxylic beetle	153:190	the largest European saproxylic beetle	153:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	2	83	theme	body	687:690	arg1	organs					692:697	body organs	687:697	body organs with gut material present in abdomens	687:735	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	3	84	theme	habitat	1192:1198	arg1	gradient					1200:1207	an urban-woodland habitat gradient	1174:1207	an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1174:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	1	85	theme	critical	365:372	arg1	issue					374:378	the critical issue	361:378	the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs,	361:495	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	7	86	theme	adult	1928:1932	arg1	beetles					1945:1951	non-feeding adult saproxylic beetles	1916:1951	non-feeding adult saproxylic beetles	1916:1951	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	2	87	theme	body	636:639	arg1	fractions					641:649	two major body fractions	626:649	two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens)	626:736	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	0	88	from	Patterns	84:91	arg1	beetle					185:190	the largest European saproxylic beetle	153:190	the largest European saproxylic beetle	153:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	0	89	theme	continent-wide	111:124	arg1	variation					140:148	continent-wide intraspecific variation	111:148	continent-wide intraspecific variation in the largest European saproxylic beetle	111:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	1	90	theme	extracellular	387:399	arg1	origin					401:406	the extracellular origin	383:406	the extracellular origin of some portion of elements, such as those present in ingested food and internal organs,	383:495	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	3	91	theme	chemical	1026:1033	arg1	K					1053:1053	K	1053:1053	K	1053:1053	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Cd					1080:1081	Cd	1080:1081	Cd	1080:1081	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Na					1056:1057	Na	1056:1057	Na	1056:1057	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Zn					1068:1069	Zn	1068:1069	Zn	1068:1069	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Ca					1045:1046	Ca	1045:1046	Ca	1045:1046	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Cu					1072:1073	Cu	1072:1073	Cu	1072:1073	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Pb					1084:1085	Pb	1084:1085	Pb	1084:1085	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Mg					1049:1050	Mg	1049:1050	Mg	1049:1050	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Mn					1060:1061	Mn	1060:1061	Mn	1060:1061	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Ni					1091:1092	Ni	1091:1092	Ni	1091:1092	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	Fe					1064:1065	Fe	1064:1065	Fe	1064:1065	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	91	theme	chemical	1026:1033	arg1	elements					1035:1042	12 chemical elements	1023:1042	12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1023:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	2	92	theme	insect	915:920	arg1	size					922:925	insect size	915:925	insect size	915:925	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	4	93	theme	local	1478:1482	arg1	conditions					1492:1501	local habitat conditions	1478:1501	local habitat conditions	1478:1501	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	0	94	theme	down	9:12	arg1	stoichiometry					21:33	Breaking down insect stoichiometry	0:33	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.	0:191	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	6	95	theme	elemental	1878:1886	arg1	pools					1888:1892	internal elemental pools	1869:1892	internal elemental pools	1869:1892	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	3	96	from	populations	1151:1161	arg1	specimens					1133:1141	specimens	1133:1141	specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia	1133:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	96	from	populations	1151:1161	arg1	abdomen					1122:1128	abdomen	1122:1128	abdomen	1122:1128	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	3	96	from	populations	1151:1161	arg1	elytra					1111:1116	the elytra	1107:1116	the elytra	1107:1116	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	2	97	from	fractions	895:903	arg1	metals					873:878	metals	873:878	metals from these two fractions	873:903	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	98	theme	Lucanus	849:855	arg1	Beetle					842:847	the Stag Beetle Lucanus cervus	833:862	the Stag Beetle Lucanus cervus	833:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	98	theme	Lucanus	849:855	arg1	insect					825:830	the largest European saproxylic insect	793:830	the largest European saproxylic insect	793:830	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	3	99	theme	entire	1229:1234	arg1	range					1251:1255	' entire distributional range	1227:1255	the species' entire distributional range from Spain to Russia	1216:1276	We examined the continent-wide variation in the concentrations of 12 chemical elements (Ca, Mg, K, Na, Mn, Fe, Zn, Cu, As, Cd, Pb and Ni) measured in the elytra and abdomen of specimens from 28 populations inhabiting an urban-woodland habitat gradient across the species' entire distributional range from Spain to Russia.					
32443193	4	100	theme	elemental	1299:1307	arg1	concentrations					1309:1322	elemental concentrations	1299:1322	elemental concentrations (except Ni and Pb)	1299:1341	Across populations, elemental concentrations (except Ni and Pb) were 2-13 times higher in abdominal samples than in elytra, and the magnitude of these differences was related to both insect size and local habitat conditions.					
32443193	0	101	theme	chitin-based	40:51	arg1	traits					76:81	chitin-based and internal elemental traits	40:81	chitin-based and internal elemental traits	40:81	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	1	102	theme	whole	326:330	arg1	bodies					345:350	whole invertebrate bodies	326:350	whole invertebrate bodies	326:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	6	103	dep	elytra	1729:1734	arg1	the					1725:1727	the	1725:1727	the	1725:1727	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	0	104	from	variation	140:148	arg1	beetle					185:190	the largest European saproxylic beetle	153:190	the largest European saproxylic beetle	153:190	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	0	105	theme	internal	57:64	arg1	traits					76:81	chitin-based and internal elemental traits	40:81	chitin-based and internal elemental traits	40:81	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	1	106	theme	bodies	345:350	arg1	traits					316:321	elemental traits	306:321	elemental traits of whole invertebrate bodies	306:350	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	7	107	theme	larval	1984:1989	arg1	stage					1991:1995	the larval stage	1980:1995	the larval stage	1980:1995	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	1	108	from	organs	489:494	arg1	present					451:457	present	451:457	present	451:457	Stoichiometric, trophic and ecotoxicological data have traditionally been acquired from patterns of variation in elemental traits of whole invertebrate bodies, whereas the critical issue of the extracellular origin of some portion of elements, such as those present in ingested food and internal organs, has been ignored.					
32443193	2	109	from	present	717:723	arg1	abdomens					728:735	abdomens	728:735	abdomens	728:735	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	7	110	theme	body	2054:2057	arg1	fat					2059:2061	residual body fat	2045:2061	residual body fat	2045:2061	We highlight that in non-feeding adult saproxylic beetles, minerals, acquired during the larval stage, may be concentrated in the large quantities of residual body fat.					
32443193	6	111	theme	elytra	1843:1848	arg1	applicability					1826:1838	the broader applicability	1814:1838	the broader applicability of elytra	1814:1848	The concentration of only six elements (Mg, K, Na, Mn, Cd and Ni) was correlated in the elytra and abdomen at the individual and population levels, implying a limitation to the broader applicability of elytra as a surrogate for internal elemental pools.					
32443193	0	112	dep	stoichiometry	21:33	arg1	correlates					97:106	correlates	97:106	correlates	97:106	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	0	112	dep	stoichiometry	21:33	arg1	Patterns					84:91	Patterns	84:91	Patterns	84:91	Breaking down insect stoichiometry into chitin-based and internal elemental traits: Patterns and correlates of continent-wide intraspecific variation in the largest European saproxylic beetle.					
32443193	2	113	theme	saproxylic	814:823	arg1	Beetle					842:847	the Stag Beetle Lucanus cervus	833:862	the Stag Beetle Lucanus cervus	833:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	113	theme	saproxylic	814:823	arg1	insect					825:830	the largest European saproxylic insect	793:830	the largest European saproxylic insect	793:830	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	114	from	abdomens	728:735	arg1	present					717:723	present	717:723	present	717:723	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	5	115	theme	higher	1576:1581	arg1	concentrations					1583:1596	higher concentrations	1576:1596	higher concentrations of trace elements (Zn, As, Cd, Pb and Ni)	1576:1638	Smaller individuals from both woodland and urban habitat tended to have higher concentrations of trace elements (Zn, As, Cd, Pb and Ni).					
32443193	2	116	theme	largest	797:803	arg1	Beetle					842:847	the Stag Beetle Lucanus cervus	833:862	the Stag Beetle Lucanus cervus	833:862	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
32443193	2	116	theme	largest	797:803	arg1	insect					825:830	the largest European saproxylic insect	793:830	the largest European saproxylic insect	793:830	Here we investigated an unexplored, yet crucial, question relating to whether, and to what degree, metals from two major body fractions: exoskeleton (elytra) and internal (body organs with gut material present in abdomens), are correlated with each other in wild populations of the largest European saproxylic insect, the Stag Beetle Lucanus cervus, and how metals from these two fractions vary with insect size and local habitat conditions.					
33771547	5	0	with	fibers	874:879	arg1	diameters					886:894	diameters	886:894	diameters ranged from 324 to 428 nm	886:920	The smooth and bead-free fibers with diameters ranged from 324 to 428 nm were obtained.					
33771547	11	1	theme	vitamin	1493:1499	arg1	D3					1501:1502	vitamin D3	1493:1502	vitamin D3 in the future	1493:1516	This study can further broaden in-vivo study and provide a reference for developing a new IDDS to carry vitamin D3 in the future.					
33771547	9	2	theme	Young	1187:1191	arg1	modulus					1195:1201	Young's modulus	1187:1201	Young's modulus	1187:1201	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	1	3	theme	global	173:178	arg1	deficiency					153:162	Vitamin D deficiency	143:162	Vitamin D deficiency	143:162	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	3	theme	global	173:178	arg1	problem					187:193	a global health problem	171:193	a global health problem	171:193	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	9	4	theme	13.07 ± 2.5 MPa	1259:1273	arg1	modulus					1195:1201	Young's modulus	1187:1201	Young's modulus	1187:1201	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	9	4	theme	13.07 ± 2.5 MPa	1259:1273	arg1	strength					1215:1222	tensile strength	1207:1222	tensile strength	1207:1222	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	0	5	theme	drug	104:107	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	5	theme	drug	104:107	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	10	6	theme	PCL	1297:1299	arg1	nanofibers					1301:1310	CA and PCL nanofibers	1290:1310	CA and PCL nanofibers	1290:1310	CA and PCL nanofibers are non-cytotoxic based on the results of the in-vitro cytotoxicity studies.					
33771547	1	7	theme	health	180:185	arg1	deficiency					153:162	Vitamin D deficiency	143:162	Vitamin D deficiency	143:162	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	7	theme	health	180:185	arg1	problem					187:193	a global health problem	171:193	a global health problem	171:193	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	8	theme	low	264:266	arg1	bioavailability					268:282	low bioavailability	264:282	low bioavailability	264:282	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	0	9	theme	cytotoxicity	121:132	arg1	studies					134:140	cytotoxicity studies	121:140	cytotoxicity studies	121:140	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	4	10	theme	nanofibrous	713:723	arg1	mat					725:727	CA nanofibrous mat	710:727	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3	710:810	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	3	11	theme	acetate	505:511	arg1	CA					514:515	the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane	479:566	CA	514:515	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	12	theme	PCL	542:544	arg1	membrane					559:566	the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane	479:566	membrane	559:566	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	11	13	theme	new	1475:1477	arg1	IDDS					1479:1482	a new IDDS	1473:1482	a new IDDS to carry vitamin D3 in the future	1473:1516	This study can further broaden in-vivo study and provide a reference for developing a new IDDS to carry vitamin D3 in the future.					
33771547	3	14	theme	ε-polycaprolactone	522:539	arg1	membrane					559:566	the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane	479:566	membrane	559:566	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	15	theme	electrospun	483:493	arg1	CA					514:515	the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane	479:566	CA	514:515	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	2	16	theme	vitamin	355:361	arg1	D3					363:364	vitamin D3	355:364	vitamin D3	355:364	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	8	17	theme	mechanical	1142:1151	arg1	properties					1153:1162	The mechanical properties	1138:1162	The mechanical properties of IDDS	1138:1170	The mechanical properties of IDDS were improved.					
33771547	4	18	theme	CA	710:711	arg1	mat					725:727	CA nanofibrous mat	710:727	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3	710:810	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	2	19	theme	current	395:401	arg1	study					403:407	the current study	391:407	the current study	391:407	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	7	20	theme	drug	1022:1025	arg1	profile					1035:1041	The controlled drug release profile	1007:1041	The controlled drug release profile	1007:1041	The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage.					
33771547	5	21	theme	bead-free	864:872	arg1	fibers					874:879	The smooth and bead-free fibers	849:879	The smooth and bead-free fibers with diameters ranged from 324 to 428 nm	849:920	The smooth and bead-free fibers with diameters ranged from 324 to 428 nm were obtained.					
33771547	3	22	theme	delivery	437:444	arg1	IDDS					454:457	IDDS	454:457	IDDS	454:457	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	22	theme	delivery	437:444	arg1	system					446:451	an implantable drug delivery system	417:451	an implantable drug delivery system (IDDS)	417:458	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	0	23	theme	D3-loaded	8:16	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	23	theme	D3-loaded	8:16	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	23	theme	D3-loaded	8:16	arg1	Characterization					77:92	Characterization	77:92	Characterization	77:92	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	3	24	theme	thin	667:670	arg1	layer					672:676	a thin layer	665:676	a thin layer of the PCL membrane (CAVD/PCL)	665:707	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	7	25	theme	zero-order	1089:1098	arg1	R2 > 0.96					1107:1115	R2 > 0.96	1107:1115	R2 > 0.96	1107:1115	The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage.					
33771547	7	25	theme	zero-order	1089:1098	arg1	model					1100:1104	the zero-order model	1085:1104	the zero-order model (R2 > 0.96)	1085:1116	The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage.					
33771547	10	26	theme	CA	1290:1291	arg1	nanofibers					1301:1310	CA and PCL nanofibers	1290:1310	CA and PCL nanofibers	1290:1310	CA and PCL nanofibers are non-cytotoxic based on the results of the in-vitro cytotoxicity studies.					
33771547	2	27	theme	delivery	328:335	arg1	system					337:342	a new drug delivery system	317:342	a new drug delivery system to deliver vitamin D3	317:364	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	10	28	theme	studies	1380:1386	arg1	results					1343:1349	the results	1339:1349	the results of the in-vitro cytotoxicity studies	1339:1386	CA and PCL nanofibers are non-cytotoxic based on the results of the in-vitro cytotoxicity studies.					
33771547	0	29	theme	Vitamin	0:6	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	29	theme	Vitamin	0:6	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	29	theme	Vitamin	0:6	arg1	Characterization					77:92	Characterization	77:92	Characterization	77:92	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	3	30	theme	membrane	689:696	arg1	layer					672:676	a thin layer	665:676	a thin layer of the PCL membrane (CAVD/PCL)	665:707	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	2	31	theme	drug	323:326	arg1	system					337:342	a new drug delivery system	317:342	a new drug delivery system to deliver vitamin D3	317:364	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	1	32	theme	several	204:210	arg1	systems					226:232	several drug delivery systems	204:232	several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity	204:303	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	0	33	theme	cellulose	30:38	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	33	theme	cellulose	30:38	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	33	theme	cellulose	30:38	arg1	Characterization					77:92	Characterization	77:92	Characterization	77:92	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	8	34	theme	IDDS	1167:1170	arg1	properties					1153:1162	The mechanical properties	1138:1162	The mechanical properties of IDDS	1138:1170	The mechanical properties of IDDS were improved.					
33771547	4	35	theme	vitamin	801:807	arg1	D3					809:810	vitamin D3	801:810	vitamin D3	801:810	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	11	36	theme	in-vivo	1420:1426	arg1	study					1428:1432	in-vivo study	1420:1432	in-vivo study	1420:1432	This study can further broaden in-vivo study and provide a reference for developing a new IDDS to carry vitamin D3 in the future.					
33771547	2	37	theme	new	319:321	arg1	system					337:342	a new drug delivery system	317:342	a new drug delivery system to deliver vitamin D3	317:364	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	1	38	theme	drug	212:215	arg1	systems					226:232	several drug delivery systems	204:232	several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity	204:303	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	0	39	theme	electrospun	18:28	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	39	theme	electrospun	18:28	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	39	theme	electrospun	18:28	arg1	Characterization					77:92	Characterization	77:92	Characterization	77:92	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	3	40	theme	vitamin	611:617	arg1	nanofiber					632:640	vitamin D3-loaded CA nanofiber	611:640	vitamin D3-loaded CA nanofiber (CAVD)	611:647	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	40	theme	vitamin	611:617	arg1	CAVD					643:646	CAVD	643:646	CAVD	643:646	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	41	theme	cellulose	495:503	arg1	CA					514:515	the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane	479:566	CA	514:515	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	1	42	theme	delivery	217:224	arg1	systems					226:232	several drug delivery systems	204:232	several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity	204:303	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	7	43	theme	release	1027:1033	arg1	profile					1035:1041	The controlled drug release profile	1007:1041	The controlled drug release profile	1007:1041	The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage.					
33771547	3	44	theme	PCL	685:687	arg1	membrane					689:696	the PCL membrane	681:696	the PCL membrane (CAVD/PCL)	681:707	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	44	theme	PCL	685:687	arg1	CAVD/PCL					699:706	CAVD/PCL	699:706	CAVD/PCL	699:706	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	45	theme	D3-loaded	619:627	arg1	nanofiber					632:640	vitamin D3-loaded CA nanofiber	611:640	vitamin D3-loaded CA nanofiber (CAVD)	611:647	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	45	theme	D3-loaded	619:627	arg1	CAVD					643:646	CAVD	643:646	CAVD	643:646	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	46	theme	implantable	420:430	arg1	IDDS					454:457	IDDS	454:457	IDDS	454:457	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	46	theme	implantable	420:430	arg1	system					446:451	an implantable drug delivery system	417:451	an implantable drug delivery system (IDDS)	417:458	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	0	47	theme	acetate/polycaprolactone	40:63	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	47	theme	acetate/polycaprolactone	40:63	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	47	theme	acetate/polycaprolactone	40:63	arg1	Characterization					77:92	Characterization	77:92	Characterization	77:92	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	4	48	theme	D3	809:810	arg1	w/w					793:795	w/w	793:795	w/w	793:795	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	4	48	theme	D3	809:810	arg1	%					790:790	6, 12, and 20%	777:790	6, 12, and 20% (w/w) of vitamin D3	777:810	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	4	48	theme	D3	809:810	arg1	D3					809:810	vitamin D3	801:810	vitamin D3	801:810	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	4	49	theme	vitamin	741:747	arg1	D3					749:750	vitamin D3	741:750	vitamin D3	741:750	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	7	50	theme	controlled	1011:1020	arg1	profile					1035:1041	The controlled drug release profile	1007:1041	The controlled drug release profile	1007:1041	The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage.					
33771547	3	51	theme	drug	432:435	arg1	IDDS					454:457	IDDS	454:457	IDDS	454:457	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	51	theme	drug	432:435	arg1	system					446:451	an implantable drug delivery system	417:451	an implantable drug delivery system (IDDS)	417:458	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	0	52	dep	nanofibers	65:74	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	52	dep	nanofibers	65:74	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	52	dep	nanofibers	65:74	arg1	studies					134:140	cytotoxicity studies	121:140	cytotoxicity studies	121:140	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	52	dep	nanofibers	65:74	arg1	Characterization					77:92	Characterization	77:92	Characterization	77:92	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	2	53	from	incentive	378:386	arg1	study					403:407	the current study	391:407	the current study	391:407	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	1	54	theme	Vitamin	143:149	arg1	deficiency					153:162	Vitamin D deficiency	143:162	Vitamin D deficiency	143:162	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	54	theme	Vitamin	143:149	arg1	problem					187:193	a global health problem	171:193	a global health problem	171:193	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	6	55	theme	fiber	941:945	arg1	diameters					947:955	The fiber diameters	937:955	The fiber diameters	937:955	The fiber diameters increased with an increase in vitamin D3 content.					
33771547	9	56	theme	CAVD/PCL	1227:1234	arg1	were161 ± 14					1242:1253	CAVD/PCL (dry) were161 ± 14	1227:1253	CAVD/PCL (dry) were161 ± 14	1227:1253	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	4	57	theme	%	790:790	arg1	concentrations					759:772	the concentrations	755:772	the concentrations of 6, 12, and 20% (w/w) of vitamin D3	755:810	CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning.					
33771547	1	58	dep	bioavailability	268:282	arg1	bioactivity					293:303	bioactivity	293:303	bioactivity	293:303	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	59	contain	carrying	238:245	arg1	systems					226:232	several drug delivery systems	204:232	several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity	204:303	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	59	contain	carrying	238:245	arg2	D					255:255	vitamin D	247:255	vitamin D	247:255	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	60	theme	D	151:151	arg1	deficiency					153:162	Vitamin D deficiency	143:162	Vitamin D deficiency	143:162	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	1	60	theme	D	151:151	arg1	problem					187:193	a global health problem	171:193	a global health problem	171:193	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	10	61	theme	cytotoxicity	1367:1378	arg1	studies					1380:1386	the in-vitro cytotoxicity studies	1354:1386	the in-vitro cytotoxicity studies	1354:1386	CA and PCL nanofibers are non-cytotoxic based on the results of the in-vitro cytotoxicity studies.					
33771547	9	62	theme	tensile	1207:1213	arg1	strength					1215:1222	tensile strength	1207:1222	tensile strength	1207:1222	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	7	63	theme	first	1125:1129	arg1	stage					1131:1135	the first stage	1121:1135	the first stage	1121:1135	The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage.					
33771547	9	64	theme	dry	1237:1239	arg1	were161 ± 14					1242:1253	CAVD/PCL (dry) were161 ± 14	1227:1253	CAVD/PCL (dry) were161 ± 14	1227:1253	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	10	65	theme	in-vitro	1358:1365	arg1	studies					1380:1386	the in-vitro cytotoxicity studies	1354:1386	the in-vitro cytotoxicity studies	1354:1386	CA and PCL nanofibers are non-cytotoxic based on the results of the in-vitro cytotoxicity studies.					
33771547	3	66	theme	CA	629:630	arg1	nanofiber					632:640	vitamin D3-loaded CA nanofiber	611:640	vitamin D3-loaded CA nanofiber (CAVD)	611:647	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	66	theme	CA	629:630	arg1	CAVD					643:646	CAVD	643:646	CAVD	643:646	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	3	67	theme	implants	590:597	arg1	core					582:585	the core	578:585	the core of implants	578:597	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	6	68	from	increase	975:982	arg1	content					998:1004	vitamin D3 content	987:1004	vitamin D3 content	987:1004	The fiber diameters increased with an increase in vitamin D3 content.					
33771547	6	69	theme	D3	995:996	arg1	content					998:1004	vitamin D3 content	987:1004	vitamin D3 content	987:1004	The fiber diameters increased with an increase in vitamin D3 content.					
33771547	1	70	theme	vitamin	247:253	arg1	D					255:255	vitamin D	247:255	vitamin D	247:255	Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity.					
33771547	0	71	theme	in-vitro	95:102	arg1	nanofibers					65:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers	0:74	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.	0:141	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	0	71	theme	in-vitro	95:102	arg1	release					109:115	in-vitro drug release	95:115	in-vitro drug release	95:115	Vitamin D3-loaded electrospun cellulose acetate/polycaprolactone nanofibers: Characterization, in-vitro drug release and cytotoxicity studies.					
33771547	2	72	theme	strong	371:376	arg1	incentive					378:386	a strong incentive	369:386	a strong incentive in the current study	369:407	Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study.					
33771547	5	73	dep	428 nm	915:920	arg1	to					912:913	to	912:913	to	912:913	The smooth and bead-free fibers with diameters ranged from 324 to 428 nm were obtained.					
33771547	9	74	theme	were161 ± 14	1242:1253	arg1	modulus					1195:1201	Young's modulus	1187:1201	Young's modulus	1187:1201	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	9	74	theme	were161 ± 14	1242:1253	arg1	strength					1215:1222	tensile strength	1207:1222	tensile strength	1207:1222	Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively.					
33771547	6	75	theme	vitamin	987:993	arg1	content					998:1004	vitamin D3 content	987:1004	vitamin D3 content	987:1004	The fiber diameters increased with an increase in vitamin D3 content.					
33771547	5	76	theme	smooth	853:858	arg1	fibers					874:879	The smooth and bead-free fibers	849:879	The smooth and bead-free fibers with diameters ranged from 324 to 428 nm	849:920	The smooth and bead-free fibers with diameters ranged from 324 to 428 nm were obtained.					
33771547	3	77	theme	nanofibrous	547:557	arg1	membrane					559:566	the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane	479:566	membrane	559:566	Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL).					
33771547	11	78	from	D3	1501:1502	arg1	future					1511:1516	future	1511:1516	future	1511:1516	This study can further broaden in-vivo study and provide a reference for developing a new IDDS to carry vitamin D3 in the future.					
32664452	2	0	theme	hydrogels	560:568	arg1	properties					532:541	the physicochemical and biological properties	497:541	the physicochemical and biological properties of the composite hydrogels	497:568	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	0	1	theme	Physiochemical	83:96	arg1	Properties					98:107	Physiochemical Properties	83:107	Physiochemical Properties	83:107	Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.					
32664452	2	2	theme	composite	550:558	arg1	hydrogels					560:568	the composite hydrogels	546:568	the composite hydrogels	546:568	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	3	theme	applicability	610:622	arg1	view					586:589	view	586:589	view of their potential applicability as tissue engineering scaffolds	586:654	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	4	4	theme	polymer	1189:1195	arg1	matrix					1197:1202	our polymer matrix	1185:1202	our polymer matrix	1185:1202	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	3	5	theme	scanning	821:828	arg1	microscopy					839:848	digital and scanning electron microscopy	809:848	microscopy	839:848	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	0	6	theme	Initial	113:119	arg1	Evaluation					139:148	Initial Cytocompatibility Evaluation	113:148	Initial Cytocompatibility Evaluation	113:148	Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.					
32664452	4	7	theme	tannic	1209:1214	arg1	acid					1216:1219	tannic acid	1209:1219	tannic acid	1209:1219	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	2	8	theme	potential	600:608	arg1	applicability					610:622	their potential applicability	594:622	their potential applicability as tissue engineering scaffolds	594:654	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	9	theme	mechanical	867:876	arg1	analysis					878:885	rheological and mechanical analysis	851:885	rheological and mechanical analysis	851:885	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	4	10	theme	specific	1160:1167	arg1	interactions					1169:1180	instead specific interactions	1152:1180	instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives	1152:1278	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	3	11	theme	umbilical	999:1007	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	11	theme	umbilical	999:1007	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	12	theme	materials	329:337	arg1	types					304:308	three types	298:308	three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp)	298:492	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	13	theme	chemical	897:904	arg1	stability					906:914	in vitro chemical stability	888:914	in vitro chemical stability	888:914	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	14	theme	digital	809:815	arg1	microscopy					839:848	digital and scanning electron microscopy	809:848	microscopy	839:848	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	4	15	theme	green-chemistry	1081:1095	arg1	approach					1097:1104	the green-chemistry approach	1077:1104	the green-chemistry approach	1077:1104	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	3	16	theme	cord	1009:1012	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	16	theme	cord	1009:1012	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	17	theme	family	322:327	arg1	materials					329:337	graphene family materials	313:337	graphene family materials (GFM)	313:343	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	17	theme	family	322:327	arg1	glycol					421:426	ethylene glycol	412:426	ethylene glycol	412:426	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	17	theme	family	322:327	arg1	GFM					340:342	GFM	340:342	GFM	340:342	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	18	theme	stability	906:914	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	0	19	theme	Cytocompatibility	121:137	arg1	Evaluation					139:148	Initial Cytocompatibility Evaluation	113:148	Initial Cytocompatibility Evaluation	113:148	Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.					
32664452	3	20	theme	stem	1042:1045	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	20	theme	stem	1042:1045	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	21	theme	graphene	313:320	arg1	materials					329:337	graphene family materials	313:337	graphene family materials (GFM)	313:343	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	21	theme	graphene	313:320	arg1	glycol					421:426	ethylene glycol	412:426	ethylene glycol	412:426	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	21	theme	graphene	313:320	arg1	GFM					340:342	GFM	340:342	GFM	340:342	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	22	from	influence	285:293	arg1	properties					532:541	the physicochemical and biological properties	497:541	the physicochemical and biological properties of the composite hydrogels	497:568	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	1	23	theme	gradient	197:204	arg1	hydrogels					222:230	gradient chitosan-matrix hydrogels	197:230	gradient chitosan-matrix hydrogels	197:230	In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing-gelling-thawing method.					
32664452	0	24	theme	Chitosan	9:16	arg1	Hydrogels					18:26	Gradient Chitosan Hydrogels	0:26	Gradient Chitosan Hydrogels	0:26	Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.					
32664452	3	25	theme	hydrogel	680:687	arg1	samples					689:695	the hydrogel samples	676:695	the hydrogel samples	676:695	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	26	theme	engineering	634:644	arg1	scaffolds					646:654	tissue engineering scaffolds	627:654	tissue engineering scaffolds	627:654	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	1	27	theme	chitosan-matrix	206:220	arg1	hydrogels					222:230	gradient chitosan-matrix hydrogels	197:230	gradient chitosan-matrix hydrogels	197:230	In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing-gelling-thawing method.					
32664452	0	28	theme	Gradient	0:7	arg1	Hydrogels					18:26	Gradient Chitosan Hydrogels	0:26	Gradient Chitosan Hydrogels	0:26	Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.					
32664452	2	29	theme	tissue	627:632	arg1	scaffolds					646:654	tissue engineering scaffolds	627:654	tissue engineering scaffolds	627:654	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	30	theme	graphene	381:388	arg1	rGO					397:399	rGO	397:399	rGO	397:399	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	30	theme	graphene	381:388	arg1	oxide					390:394	reduced graphene oxide	373:394	reduced graphene oxide (rGO)	373:400	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	31	theme	ethylene	412:419	arg1	glycol					421:426	ethylene glycol	412:426	ethylene glycol	412:426	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	31	theme	ethylene	412:419	arg1	materials					329:337	graphene family materials	313:337	graphene family materials (GFM)	313:343	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	32	theme	X-ray	730:734	arg1	spectroscopy					750:761	X-ray photoelectron spectroscopy	730:761	X-ray photoelectron spectroscopy	730:761	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	33	theme	mesenchymal	1030:1040	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	33	theme	mesenchymal	1030:1040	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	34	theme	jelly	1024:1028	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	34	theme	jelly	1024:1028	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	35	dep	oxide	361:365	arg1	GO					368:369	GO	368:369	GO	368:369	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	35	dep	oxide	361:365	arg1	i.e.					346:349	i.e.	346:349	i.e.	346:349	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	4	36	theme	toxic	1118:1122	arg1	agents					1138:1143	toxic cross-linking agents	1118:1143	toxic cross-linking agents	1118:1143	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	0	37	theme	Graphene	42:49	arg1	Derivatives					51:61	Graphene Derivatives	42:61	Graphene Derivatives	42:61	Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation.					
32664452	4	38	theme	cross-linking	1124:1136	arg1	agents					1138:1143	toxic cross-linking agents	1118:1143	toxic cross-linking agents	1118:1143	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	1	39	theme	hydrogels	222:230	arg1	preparation					182:192	preparation	182:192	preparation of gradient chitosan-matrix hydrogels	182:230	In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing-gelling-thawing method.					
32664452	1	40	theme	novel	242:246	arg1	method					273:278	a novel freezing-gelling-thawing method	240:278	a novel freezing-gelling-thawing method	240:278	In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing-gelling-thawing method.					
32664452	3	41	with	assays	932:937	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	41	with	assays	932:937	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	42	dep	in	888:889	arg1	vitro					891:895	vitro	891:895	vitro	891:895	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	1	43	theme	freezing-gelling-thawing	248:271	arg1	method					273:278	a novel freezing-gelling-thawing method	240:278	a novel freezing-gelling-thawing method	240:278	In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing-gelling-thawing method.					
32664452	2	44	theme	graphene	437:444	arg1	GO-PEG					453:458	GO-PEG	453:458	GO-PEG	453:458	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	44	theme	graphene	437:444	arg1	oxide					446:450	graphene oxide	437:450	graphene oxide (GO-PEG)	437:459	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	45	theme	X-ray	764:768	arg1	diffractometry					770:783	X-ray diffractometry	764:783	X-ray diffractometry	764:783	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	46	theme	initial	951:957	arg1	evaluation					977:986	initial cytocompatibility evaluation	951:986	initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	951:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	47	theme	photoelectron	736:748	arg1	spectroscopy					750:761	X-ray photoelectron spectroscopy	730:761	X-ray photoelectron spectroscopy	730:761	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	48	theme	biological	521:530	arg1	properties					532:541	the physicochemical and biological properties	497:541	the physicochemical and biological properties of the composite hydrogels	497:568	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	5	49	theme	gradient	1326:1333	arg1	hydrogels					1335:1343	the gradient hydrogels	1322:1343	the gradient hydrogels modified with GO-PEG and HAp	1322:1372	It was shown that the most promising are the gradient hydrogels modified with GO-PEG and HAp.					
32664452	5	49	theme	gradient	1326:1333	arg1	promising					1308:1316	promising	1308:1316	promising	1308:1316	It was shown that the most promising are the gradient hydrogels modified with GO-PEG and HAp.					
32664452	4	50	theme	graphene	1259:1266	arg1	derivatives					1268:1278	graphene derivatives	1259:1278	graphene derivatives	1259:1278	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	4	51	theme	physical	1232:1239	arg1	cross-linker					1241:1252	non-toxic physical cross-linker	1222:1252	non-toxic physical cross-linker	1222:1252	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	3	52	theme	spectroscopy	750:761	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	53	theme	in	888:889	arg1	stability					906:914	in vitro chemical stability	888:914	in vitro chemical stability	888:914	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	4	54	theme	matrix	1197:1202	arg1	interactions					1169:1180	instead specific interactions	1152:1180	instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives	1152:1278	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	2	55	theme	graphene	352:359	arg1	oxide					361:365	graphene oxide	352:365	graphene oxide (GO)	352:370	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	56	theme	rheological	851:861	arg1	analysis					878:885	rheological and mechanical analysis	851:885	rheological and mechanical analysis	851:885	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	57	theme	infrared	786:793	arg1	spectroscopy					795:806	infrared spectroscopy	786:806	infrared spectroscopy	786:806	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	58	theme	physicochemical	501:515	arg1	properties					532:541	the physicochemical and biological properties	497:541	the physicochemical and biological properties of the composite hydrogels	497:568	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	59	theme	types	304:308	arg1	influence					285:293	The influence	281:293	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels	281:568	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	60	theme	bioactivity	920:930	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	61	theme	electron	830:837	arg1	microscopy					839:848	digital and scanning electron microscopy	809:848	microscopy	839:848	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	62	theme	spectroscopy	795:806	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	63	with	evaluation	977:986	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	63	with	evaluation	977:986	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	64	theme	human	993:997	arg1	hUC-MSCs					1054:1061	hUC-MSCs	1054:1061	hUC-MSCs	1054:1061	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	64	theme	human	993:997	arg1	cells					1047:1051	human umbilical cord Wharton's jelly mesenchymal stem cells	993:1051	human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	993:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	2	65	theme	reduced	373:379	arg1	rGO					397:399	rGO	397:399	rGO	397:399	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	2	65	theme	reduced	373:379	arg1	oxide					390:394	reduced graphene oxide	373:394	reduced graphene oxide (rGO)	373:400	The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds.					
32664452	3	66	theme	microscopy	839:848	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	67	theme	diffractometry	770:783	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	4	68	theme	non-toxic	1222:1230	arg1	cross-linker					1241:1252	non-toxic physical cross-linker	1222:1252	non-toxic physical cross-linker	1222:1252	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	3	69	theme	cytocompatibility	959:975	arg1	evaluation					977:986	initial cytocompatibility evaluation	951:986	initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs)	951:1062	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	3	70	theme	analysis	878:885	arg1	assays					932:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays	730:937	The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton's jelly mesenchymal stem cells (hUC-MSCs).					
32664452	4	71	with	interactions	1169:1180	arg1	cross-linker					1241:1252	non-toxic physical cross-linker	1222:1252	non-toxic physical cross-linker	1222:1252	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	4	71	with	interactions	1169:1180	arg1	acid					1216:1219	tannic acid	1209:1219	tannic acid	1209:1219	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
32664452	4	71	with	interactions	1169:1180	arg1	derivatives					1268:1278	graphene derivatives	1259:1278	graphene derivatives	1259:1278	We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives.					
33848548	4	0	theme	subsequent	825:834	arg1	process					851:857	the subsequent photocatalysis process	821:857	the subsequent photocatalysis process	821:857	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	1	1	theme	cellulose	205:213	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	1	theme	cellulose	205:213	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	2	theme	red	296:298	arg1	K-2G					300:303	the reactive brilliant red K-2G	273:303	the reactive brilliant red K-2G from aqueous solution	273:325	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	0	3	theme	dye	98:100	arg1	removal					102:108	dye removal	98:108	dye removal	98:108	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	1	4	theme	@	214:214	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	4	theme	@	214:214	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	4	5	theme	first	701:705	arg1	scenario					707:714	the first scenario	697:714	the first scenario	697:714	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	4	6	theme	equilibrium	968:978	arg1	state					980:984	equilibrium state	968:984	equilibrium state (about 180 min)	968:1000	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	4	6	theme	equilibrium	968:978	arg1	min					997:999	about 180 min	987:999	about 180 min	987:999	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	6	7	theme	wastewater	1299:1308	arg1	treatment					1272:1280	the treatment	1268:1280	the treatment of dyes polluted wastewater	1268:1308	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	3	8	theme	photocatalysis	618:631	arg1	process					633:639	photocatalysis process	618:639	photocatalysis process	618:639	Batch of experiments were conducted in two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process.					
33848548	4	9	theme	photocatalysis	836:849	arg1	process					851:857	the subsequent photocatalysis process	821:857	the subsequent photocatalysis process	821:857	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	1	10	theme	aqueous	310:316	arg1	solution					318:325	aqueous solution	310:325	aqueous solution	310:325	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	3	11	dep	scenarios	584:592	arg1	adsorption					595:604	adsorption	595:604	two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process	580:690	Batch of experiments were conducted in two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process.					
33848548	5	12	theme	removal	1063:1069	arg1	mg·g-1					1096:1101	about 558.1 mg·g-1	1084:1101	about 558.1 mg·g-1	1084:1101	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	5	12	theme	removal	1063:1069	arg1	capacity					1071:1078	the removal capacity	1059:1078	the removal capacity	1059:1078	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	1	13	theme	graphene	215:222	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	13	theme	graphene	215:222	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	4	14	theme	expected	774:781	arg1	capacity					794:801	the expected adsorption capacity	770:801	the expected adsorption capacity	770:801	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	4	14	theme	expected	774:781	arg1	mg·g-1					813:818	447.0 mg·g-1	807:818	447.0 mg·g-1	807:818	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	2	15	theme	photocatalysis	498:511	arg1	performance					513:523	photocatalysis performance	498:523	photocatalysis performance	498:523	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	5	16	theme	equilibrium	1184:1194	arg1	state					1196:1200	equilibrium state	1184:1200	equilibrium state	1184:1200	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	1	17	from	solution	318:325	arg1	K-2G					300:303	the reactive brilliant red K-2G	273:303	the reactive brilliant red K-2G from aqueous solution	273:325	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	2	18	theme	cellulose	360:368	arg1	addition					332:339	The addition	328:339	The addition of dicarboxyl acid cellulose	328:368	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	0	19	theme	Adsorption-photocatalysis	0:24	arg1	performance					26:36	Adsorption-photocatalysis performance	0:36	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.	0:109	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	2	20	theme	acid	355:358	arg1	cellulose					360:368	dicarboxyl acid cellulose	344:368	dicarboxyl acid cellulose	344:368	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	0	21	theme	polyaniline/dicarboxyl	41:62	arg1	oxide					88:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	4	22	theme	removal	907:913	arg1	capacity					915:922	the removal capacity	903:922	the removal capacity	903:922	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	1	23	theme	novel	113:117	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	23	theme	novel	113:117	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	5	24	from	min	1115:1117	arg1	mg·g-1					1096:1101	about 558.1 mg·g-1	1084:1101	about 558.1 mg·g-1	1084:1101	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	5	24	from	min	1115:1117	arg1	capacity					1071:1078	the removal capacity	1059:1078	the removal capacity	1059:1078	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	4	25	theme	adsorption	717:726	arg1	equilibrium					728:738	adsorption equilibrium	717:738	adsorption equilibrium	717:738	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	2	26	theme	excellent	473:481	arg1	adsorption					483:492	excellent adsorption	473:492	excellent adsorption	473:492	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	5	27	theme	simultaneous	1011:1022	arg1	process					1050:1056	the simultaneous adsorption-photocatalytic process	1007:1056	the simultaneous adsorption-photocatalytic process	1007:1056	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	5	28	theme	adsorption-photocatalytic	1024:1048	arg1	process					1050:1056	the simultaneous adsorption-photocatalytic process	1007:1056	the simultaneous adsorption-photocatalytic process	1007:1056	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	1	29	theme	3-D	119:121	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	29	theme	3-D	119:121	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	6	30	theme	excellent	1225:1233	arg1	reusability					1235:1245	excellent reusability	1225:1245	excellent reusability	1225:1245	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	0	31	theme	cellulose	69:77	arg1	oxide					88:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	3	32	theme	experiments	550:560	arg1	Batch					541:545	Batch	541:545	Batch of experiments	541:560	Batch of experiments were conducted in two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process.					
33848548	6	33	theme	PANI-DCC	1203:1210	arg1	@					1211:1211	PANI-DCC@GO	1203:1213	PANI-DCC@GO	1203:1213	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	0	34	theme	acid	64:67	arg1	oxide					88:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	4	35	theme	adsorption	783:792	arg1	capacity					794:801	the expected adsorption capacity	770:801	the expected adsorption capacity	770:801	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	4	35	theme	adsorption	783:792	arg1	mg·g-1					813:818	447.0 mg·g-1	807:818	447.0 mg·g-1	807:818	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	2	36	theme	nanocomposite	443:455	arg1	structure					400:408	structure	400:408	structure	400:408	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	2	36	theme	nanocomposite	443:455	arg1	morphology					388:397	morphology	388:397	morphology	388:397	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	2	36	theme	nanocomposite	443:455	arg1	dispersity					425:434	dispersity	425:434	dispersity	425:434	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	2	36	theme	nanocomposite	443:455	arg1	stability					411:419	stability	411:419	stability	411:419	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	0	37	theme	graphene	79:86	arg1	oxide					88:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	3	38	theme	simultaneous	645:656	arg1	process					684:690	simultaneous adsorption-photocatalysis process	645:690	simultaneous adsorption-photocatalysis process	645:690	Batch of experiments were conducted in two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process.					
33848548	1	39	theme	biopolymer-based	123:138	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	39	theme	biopolymer-based	123:138	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	0	40	theme	@	78:78	arg1	oxide					88:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	polyaniline/dicarboxyl acid cellulose@graphene oxide	41:92	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	3	41	theme	adsorption-photocatalysis	658:682	arg1	process					684:690	simultaneous adsorption-photocatalysis process	645:690	simultaneous adsorption-photocatalysis process	645:690	Batch of experiments were conducted in two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process.					
33848548	1	42	theme	adsorption-photocatalytic	140:164	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	42	theme	adsorption-photocatalytic	140:164	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	6	43	theme	polluted	1290:1297	arg1	wastewater					1299:1308	dyes polluted wastewater	1285:1308	dyes polluted wastewater	1285:1308	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	2	44	dep	morphology	388:397	arg1	the					384:386	the	384:386	the	384:386	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	6	45	theme	dyes	1285:1288	arg1	wastewater					1299:1308	dyes polluted wastewater	1285:1308	dyes polluted wastewater	1285:1308	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	2	46	theme	dicarboxyl	344:353	arg1	cellulose					360:368	dicarboxyl acid cellulose	344:368	dicarboxyl acid cellulose	344:368	The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product.					
33848548	5	47	theme	total	1127:1131	arg1	capacity					1141:1148	the total removal capacity	1123:1148	the total removal capacity	1123:1148	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	6	48	contain	had	1251:1253	arg2	potential					1255:1263	potential	1255:1263	potential	1255:1263	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	6	48	contain	had	1251:1253	arg1	@					1211:1211	PANI-DCC@GO	1203:1213	PANI-DCC@GO	1203:1213	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	5	49	theme	removal	1133:1139	arg1	capacity					1141:1148	the total removal capacity	1123:1148	the total removal capacity	1123:1148	For the simultaneous adsorption-photocatalytic process, the removal capacity was about 558.1 mg·g-1 at about 25 min and the total removal capacity could reach to 733.3 mg·g-1 under equilibrium state.					
33848548	6	50	dep	@	1211:1211	arg1	GO					1212:1213	GO	1212:1213	GO	1212:1213	PANI-DCC@GO exhibited excellent reusability and had potential in the treatment of dyes polluted wastewater.					
33848548	1	51	theme	polyaniline/dicarboxyl	177:198	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	51	theme	polyaniline/dicarboxyl	177:198	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	52	theme	reactive	277:284	arg1	K-2G					300:303	the reactive brilliant red K-2G	273:303	the reactive brilliant red K-2G from aqueous solution	273:325	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	0	53	theme	oxide	88:92	arg1	performance					26:36	Adsorption-photocatalysis performance	0:36	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.	0:109	Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.					
33848548	4	54	theme	light	881:885	arg1	irradiation					887:897	light irradiation	881:897	light irradiation	881:897	For the first scenario, adsorption equilibrium can be reached within 25 min, the expected adsorption capacity was 447.0 mg·g-1; the subsequent photocatalysis process was carried out under light irradiation and the removal capacity could further improve to 729.0 mg·g-1 under equilibrium state (about 180 min).					
33848548	1	55	theme	acid	200:203	arg1	composite					166:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite	111:174	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	55	theme	acid	200:203	arg1	oxide					224:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	polyaniline/dicarboxyl acid cellulose@graphene oxide	177:228	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
33848548	1	56	theme	brilliant	286:294	arg1	K-2G					300:303	the reactive brilliant red K-2G	273:303	the reactive brilliant red K-2G from aqueous solution	273:325	A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution.					
34569111	5	0	theme	active	1058:1063	arg1	state					1075:1079	the active (agonist) state	1054:1079	the active (agonist) state of the TLR4	1054:1091	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	6	1	theme	TLR4	1233:1236	arg1	pathways					1252:1259	the TLR4 innate immune pathways	1229:1259	the TLR4 innate immune pathways	1229:1259	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	1	2	theme	/myeloid	126:133	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	2	theme	/myeloid	126:133	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	5	3	theme	TLR4	961:964	arg1	structures					990:999	reasonable 3D structures	976:999	reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane	976:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	5	3	theme	TLR4	961:964	arg1	models					966:971	TLR4 models	961:971	TLR4 models	961:971	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	0	4	from	Model	10:14	arg1	Environment					82:92	a Membrane Environment	71:92	a Membrane Environment	71:92	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	1	5	theme	differentiation	135:149	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	5	theme	differentiation	135:149	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	6	theme	paramount	216:224	arg1	importance					226:235	paramount importance	216:235	paramount importance	216:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	4	7	theme	transmembrane	756:768	arg1	behavior					777:784	the transmembrane domain behavior	752:784	the transmembrane domain behavior in different membrane environments	752:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	4	8	theme	dimerization	864:875	arg1	patterns					877:884	the dimerization patterns	860:884	the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain	860:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	3	9	theme	docking	484:490	arg1	simulations					521:531	molecular docking, all-atom molecular dynamics simulations	474:531	simulations	521:531	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	4	10	theme	functional	712:721	arg1	insights					738:745	functional and structural insights	712:745	functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain	712:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	1	11	theme	importance	226:235	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	11	theme	importance	226:235	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	0	12	theme	Membrane	73:80	arg1	Environment					82:92	a Membrane Environment	71:92	a Membrane Environment	71:92	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	4	13	from	movement	846:853	arg1	environments					808:819	different membrane environments	789:819	different membrane environments	789:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	6	14	theme	relevant	1190:1197	arg1	aspects					1209:1215	relevant molecular aspects	1190:1215	relevant molecular aspects involved in the TLR4 innate immune pathways	1190:1259	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	4	15	theme	structural	727:736	arg1	insights					738:745	functional and structural insights	712:745	functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain	712:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	3	16	theme	ab-initio	450:458	arg1	calculations					460:471	ab-initio calculations	450:471	ab-initio calculations	450:471	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	4	17	theme	ectodomain	826:835	arg1	movement					846:853	the ectodomain bouncing movement	822:853	the ectodomain bouncing movement	822:853	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	3	18	theme	all-atom	493:500	arg1	dynamics					512:519	all-atom molecular dynamics	493:519	all-atom molecular dynamics	493:519	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	1	19	theme	factor	151:156	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	19	theme	factor	151:156	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	0	20	theme	Toll-like	41:49	arg1	Dimer					62:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	5	21	theme	TLR4	1088:1091	arg1	state					1075:1079	the active (agonist) state	1054:1079	the active (agonist) state of the TLR4	1054:1091	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	6	22	theme	immune	1245:1250	arg1	pathways					1252:1259	the TLR4 innate immune pathways	1229:1259	the TLR4 innate immune pathways	1229:1259	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	3	23	theme	molecular	502:510	arg1	dynamics					512:519	all-atom molecular dynamics	493:519	all-atom molecular dynamics	493:519	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	5	24	dep	architecture	1026:1037	arg1	pointing					1098:1105	pointing	1098:1105	pointing to a signal transduction mechanism across cell membrane	1098:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	5	24	dep	architecture	1026:1037	arg1	accounting					1039:1048	accounting	1039:1048	accounting for the active (agonist) state of the TLR4	1039:1091	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	2	25	theme	immune	408:413	arg1	cascade					424:430	the immune response cascade	404:430	the immune response cascade	404:430	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	6	26	theme	innate	1238:1243	arg1	pathways					1252:1259	the TLR4 innate immune pathways	1229:1259	the TLR4 innate immune pathways	1229:1259	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	5	27	theme	cell	1149:1152	arg1	membrane					1154:1161	cell membrane	1149:1161	cell membrane	1149:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	3	28	theme	thermodynamics	538:551	arg1	calculations					553:564	thermodynamics calculations	538:564	thermodynamics calculations	538:564	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	0	29	theme	Full-Atom	0:8	arg1	Model					10:14	Full-Atom Model	0:14	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.	0:93	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	1	30	theme	therapeutic	240:250	arg1	target					252:257	therapeutic target	240:257	therapeutic target	240:257	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	4	31	theme	bouncing	837:844	arg1	movement					846:853	the ectodomain bouncing movement	822:853	the ectodomain bouncing movement	822:853	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	1	32	theme	MD-2	161:164	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	32	theme	MD-2	161:164	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	4	33	from	patterns	877:884	arg1	environments					808:819	different membrane environments	789:819	different membrane environments	789:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	4	34	theme	receptor	926:933	arg1	domain					935:940	the intracellular Toll/Interleukin-1 receptor domain	889:940	the intracellular Toll/Interleukin-1 receptor domain	889:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	3	35	theme	active	626:631	arg1	complex					643:649	the active full TLR4 complex	622:649	the active full TLR4 complex embedded into a realistic membrane to date	622:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	5	36	theme	TLR4/MD-2/LPS	1010:1022	arg1	architecture					1026:1037	the (TLR4/MD-2/LPS)2 architecture	1005:1037	the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane	1005:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	4	37	theme	Toll/Interleukin-1	907:924	arg1	domain					935:940	the intracellular Toll/Interleukin-1 receptor domain	889:940	the intracellular Toll/Interleukin-1 receptor domain	889:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	2	38	dep	bacteria	304:311	arg1	LPS					333:335	LPS	333:335	LPS	333:335	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	2	38	dep	bacteria	304:311	arg1	lipopolysaccharide					313:330	lipopolysaccharide	313:330	Gram-negative bacteria lipopolysaccharide (LPS)	290:336	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	1	39	theme	innate	167:172	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	39	theme	innate	167:172	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	0	40	theme	LPS-Bound	31:39	arg1	Dimer					62:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	2	41	theme	bacteria	304:311	arg1	binding					279:285	binding	279:285	binding of Gram-negative bacteria lipopolysaccharide (LPS)	279:336	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	2	42	theme	membrane	365:372	arg1	composition					374:384	the membrane composition	361:384	the membrane composition	361:384	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	4	43	theme	membrane	799:806	arg1	environments					808:819	different membrane environments	789:819	different membrane environments	789:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	1	44	theme	immunity	174:181	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	44	theme	immunity	174:181	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	0	45	theme	Agonist	23:29	arg1	Dimer					62:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	5	46	theme	signal	1112:1117	arg1	mechanism					1132:1140	a signal transduction mechanism	1110:1140	a signal transduction mechanism across cell membrane	1110:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	4	47	theme	domain	935:940	arg1	behavior					777:784	the transmembrane domain behavior	752:784	the transmembrane domain behavior in different membrane environments	752:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	4	47	theme	domain	935:940	arg1	patterns					877:884	the dimerization patterns	860:884	the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain	860:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	4	47	theme	domain	935:940	arg1	movement					846:853	the ectodomain bouncing movement	822:853	the ectodomain bouncing movement	822:853	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	4	48	theme	different	789:797	arg1	environments					808:819	different membrane environments	789:819	different membrane environments	789:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	0	49	theme	Receptor	51:58	arg1	Dimer					62:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	the Agonist LPS-Bound Toll-like Receptor 4 Dimer	19:66	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	5	50	theme	transduction	1119:1130	arg1	mechanism					1132:1140	a signal transduction mechanism	1110:1140	a signal transduction mechanism across cell membrane	1110:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	6	51	theme	TLR4	1299:1302	arg1	modulators					1304:1313	new TLR4 modulators	1295:1313	new TLR4 modulators	1295:1313	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	5	52	theme	reasonable	976:985	arg1	structures					990:999	reasonable 3D structures	976:999	reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane	976:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	5	52	theme	reasonable	976:985	arg1	models					966:971	TLR4 models	961:971	TLR4 models	961:971	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	1	53	theme	Toll-like	99:107	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	53	theme	Toll-like	99:107	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	2	54	theme	response	415:422	arg1	cascade					424:430	the immune response cascade	404:430	the immune response cascade	404:430	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	6	55	theme	new	1295:1297	arg1	modulators					1304:1313	new TLR4 modulators	1295:1313	new TLR4 modulators	1295:1313	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	3	56	theme	complex	643:649	arg1	models					612:617	the most realistic and complete 3D models	577:617	the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date	577:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	3	57	theme	molecular	474:482	arg1	simulations					521:531	molecular docking, all-atom molecular dynamics simulations	474:531	simulations	521:531	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	1	58	theme	receptor	109:116	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	58	theme	receptor	109:116	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	4	59	theme	intracellular	893:905	arg1	domain					935:940	the intracellular Toll/Interleukin-1 receptor domain	889:940	the intracellular Toll/Interleukin-1 receptor domain	889:940	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	4	60	theme	domain	770:775	arg1	behavior					777:784	the transmembrane domain behavior	752:784	the transmembrane domain behavior in different membrane environments	752:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	3	61	theme	complete	600:607	arg1	models					612:617	the most realistic and complete 3D models	577:617	the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date	577:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	2	62	theme	Gram-negative	290:302	arg1	bacteria					304:311	Gram-negative bacteria lipopolysaccharide (LPS)	290:336	Gram-negative bacteria lipopolysaccharide (LPS)	290:336	Its assembly, upon binding of Gram-negative bacteria lipopolysaccharide (LPS), and also dependent on the membrane composition, finally triggers the immune response cascade.					
34569111	6	63	theme	modulators	1304:1313	arg1	discovery					1282:1290	the discovery	1278:1290	the discovery of new TLR4 modulators	1278:1313	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34569111	5	64	theme	3D	987:988	arg1	structures					990:999	reasonable 3D structures	976:999	reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane	976:1161	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	5	64	theme	3D	987:988	arg1	models					966:971	TLR4 models	961:971	TLR4 models	961:971	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	3	65	theme	full	633:636	arg1	complex					643:649	the active full TLR4 complex	622:649	the active full TLR4 complex embedded into a realistic membrane to date	622:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	3	66	theme	3D	609:610	arg1	models					612:617	the most realistic and complete 3D models	577:617	the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date	577:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	5	67	theme	agonist	1066:1072	arg1	state					1075:1079	the active (agonist) state	1054:1079	the active (agonist) state of the TLR4	1054:1091	Our work provides TLR4 models as reasonable 3D structures for the (TLR4/MD-2/LPS)2 architecture accounting for the active (agonist) state of the TLR4, and pointing to a signal transduction mechanism across cell membrane.					
34569111	3	68	dep	simulations	521:531	arg1	dynamics					512:519	all-atom molecular dynamics	493:519	all-atom molecular dynamics	493:519	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	0	69	theme	Dimer	62:66	arg1	Model					10:14	Full-Atom Model	0:14	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.	0:93	Full-Atom Model of the Agonist LPS-Bound Toll-like Receptor 4 Dimer in a Membrane Environment.					
34569111	3	70	theme	TLR4	638:641	arg1	complex					643:649	the active full TLR4 complex	622:649	the active full TLR4 complex embedded into a realistic membrane to date	622:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	3	71	theme	realistic	586:594	arg1	models					612:617	the most realistic and complete 3D models	577:617	the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date	577:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	4	72	from	behavior	777:784	arg1	environments					808:819	different membrane environments	789:819	different membrane environments	789:819	Our studies give functional and structural insights into the transmembrane domain behavior in different membrane environments, the ectodomain bouncing movement, and the dimerization patterns of the intracellular Toll/Interleukin-1 receptor domain.					
34569111	1	73	theme	TLR4	121:124	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	73	theme	TLR4	121:124	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	74	theme	membrane	195:202	arg1	receptor					204:211	a membrane receptor	193:211	a membrane receptor of paramount importance	193:235	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	1	74	theme	membrane	195:202	arg1	system					183:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system	95:188	The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) innate immunity system is a membrane receptor of paramount importance as therapeutic target.					
34569111	3	75	dep	complex	643:649	arg1	embedded					651:658	embedded	651:658	embedded	651:658	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	3	76	theme	realistic	667:675	arg1	membrane					677:684	a realistic membrane	665:684	a realistic membrane to date	665:692	We have combined ab-initio calculations, molecular docking, all-atom molecular dynamics simulations, and thermodynamics calculations to provide the most realistic and complete 3D models of the active full TLR4 complex embedded into a realistic membrane to date.					
34569111	6	77	theme	molecular	1199:1207	arg1	aspects					1209:1215	relevant molecular aspects	1190:1215	relevant molecular aspects involved in the TLR4 innate immune pathways	1190:1259	These observations unveil relevant molecular aspects involved in the TLR4 innate immune pathways and will promote the discovery of new TLR4 modulators.					
34364561	0	0	theme	neuronal	69:76	arg1	scaffold					86:93	neuronal network scaffold	69:93	neuronal network scaffold based on chitosan and graphite nanoplatelets	69:138	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	5	1	theme	good	757:760	arg1	conductivity					773:784	good electrical conductivity	757:784	good electrical conductivity	757:784	The presence of GNP turned out to improve the strength of the microparticles while conferring good electrical conductivity and ameliorating their stability in aqueous environment.					
34364561	1	2	theme	neuronal	278:285	arg1	cells					287:291	neuronal cells	278:291	neuronal cells	278:291	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	4	3	theme	Microscopy	573:582	arg1	micrographs					584:594	Optical and Field Emission Scanning Electron Microscopy micrographs	528:594	Optical and Field Emission Scanning Electron Microscopy micrographs	528:594	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	5	4	theme	electrical	762:771	arg1	conductivity					773:784	good electrical conductivity	757:784	good electrical conductivity	757:784	The presence of GNP turned out to improve the strength of the microparticles while conferring good electrical conductivity and ameliorating their stability in aqueous environment.					
34364561	0	5	dep	chitosan	104:111	arg1	nanoplatelets					126:138	nanoplatelets	126:138	nanoplatelets	126:138	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	4	6	theme	Electron	564:571	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy	540:582	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	0	7	theme	network	78:84	arg1	scaffold					86:93	neuronal network scaffold	69:93	neuronal network scaffold based on chitosan and graphite nanoplatelets	69:138	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	6	8	theme	electrophysiological	930:949	arg1	analysis					951:958	a preliminary electrophysiological analysis	916:958	a preliminary electrophysiological analysis	916:958	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	7	9	theme	networks	1162:1169	arg1	screening					1198:1206	drug screening	1193:1206	drug screening	1193:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	7	9	theme	networks	1162:1169	arg1	dynamics					1171:1178	networks dynamics	1162:1178	networks dynamics analysis and drug screening	1162:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	7	9	theme	networks	1162:1169	arg1	analysis					1180:1187	analysis	1180:1187	analysis	1180:1187	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	6	10	theme	preliminary	918:928	arg1	analysis					951:958	a preliminary electrophysiological analysis	916:958	a preliminary electrophysiological analysis	916:958	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	3	11	theme	CHI/GNP	504:510	arg1	microparticles					512:525	CHI/GNP microparticles	504:525	CHI/GNP microparticles	504:525	An air-assisted jetting technique followed by physical crosslinking is employed to obtain CHI/GNP microparticles.					
34364561	5	12	theme	microparticles	725:738	arg1	strength					709:716	the strength	705:716	the strength of the microparticles	705:738	The presence of GNP turned out to improve the strength of the microparticles while conferring good electrical conductivity and ameliorating their stability in aqueous environment.					
34364561	5	13	from	stability	809:817	arg1	environment					830:840	aqueous environment	822:840	aqueous environment	822:840	The presence of GNP turned out to improve the strength of the microparticles while conferring good electrical conductivity and ameliorating their stability in aqueous environment.					
34364561	7	14	theme	models	1151:1156	arg1	development					1106:1116	the development	1102:1116	the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening	1102:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	1	15	theme	novel	155:159	arg1	microparticles					171:184	novel composite microparticles	155:184	novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP)	155:241	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	1	15	theme	novel	155:159	arg1	scaffolds					264:272	3D scaffolds	261:272	3D scaffolds for neuronal cells	261:291	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	0	16	theme	facile	2:7	arg1	approach					9:16	A facile approach	0:16	A facile approach for the development of high mechanical strength	0:64	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	4	17	theme	Emission	546:553	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy	540:582	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	7	18	theme	neuronal	1133:1140	arg1	models					1151:1156	advanced 3D neuronal in vitro models	1121:1156	advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening	1121:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	1	19	theme	composite	161:169	arg1	microparticles					171:184	novel composite microparticles	155:184	novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP)	155:241	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	1	19	theme	composite	161:169	arg1	scaffolds					264:272	3D scaffolds	261:272	3D scaffolds for neuronal cells	261:291	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	4	20	theme	Field	540:544	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy	540:582	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	7	21	theme	in	1142:1143	arg1	models					1151:1156	advanced 3D neuronal in vitro models	1121:1156	advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening	1121:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	5	22	theme	aqueous	822:828	arg1	environment					830:840	aqueous environment	822:840	aqueous environment	822:840	The presence of GNP turned out to improve the strength of the microparticles while conferring good electrical conductivity and ameliorating their stability in aqueous environment.					
34364561	6	23	theme	microparticles	1016:1029	arg1	effectiveness					975:987	the effectiveness	971:987	the effectiveness of the developed composite microparticles	971:1029	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	6	23	theme	microparticles	1016:1029	arg1	scaffold					1036:1043	a scaffold	1034:1043	a scaffold for neuron growth	1034:1061	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	3	24	theme	jetting	430:436	arg1	technique					438:446	An air-assisted jetting technique	414:446	An air-assisted jetting technique followed by physical crosslinking	414:480	An air-assisted jetting technique followed by physical crosslinking is employed to obtain CHI/GNP microparticles.					
34364561	4	25	theme	Scanning	555:562	arg1	Microscopy					573:582	Field Emission Scanning Electron Microscopy	540:582	Field Emission Scanning Electron Microscopy	540:582	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	7	26	theme	3D	1130:1131	arg1	models					1151:1156	advanced 3D neuronal in vitro models	1121:1156	advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening	1121:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	4	27	theme	GNP	629:631	arg1	distribution					613:624	a uniform distribution	603:624	a uniform distribution of GNP within the CHI porous matrix	603:660	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	1	28	theme	3D	261:262	arg1	microparticles					171:184	novel composite microparticles	155:184	novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP)	155:241	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	1	28	theme	3D	261:262	arg1	scaffolds					264:272	3D scaffolds	261:272	3D scaffolds for neuronal cells	261:291	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	6	29	theme	composite	1006:1014	arg1	microparticles					1016:1029	the developed composite microparticles	992:1029	the developed composite microparticles	992:1029	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	4	30	theme	CHI	644:646	arg1	matrix					655:660	the CHI porous matrix	640:660	the CHI porous matrix	640:660	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	7	31	dep	in	1142:1143	arg1	vitro					1145:1149	vitro	1145:1149	vitro	1145:1149	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	6	32	theme	developed	996:1004	arg1	microparticles					1016:1029	the developed composite microparticles	992:1029	the developed composite microparticles	992:1029	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	6	33	theme	neuron	1049:1054	arg1	growth					1056:1061	neuron growth	1049:1061	neuron growth	1049:1061	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	7	34	dep	dynamics	1171:1178	arg1	screening					1198:1206	drug screening	1193:1206	drug screening	1193:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	7	34	dep	dynamics	1171:1178	arg1	dynamics					1171:1178	networks dynamics	1162:1178	networks dynamics analysis and drug screening	1162:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	7	34	dep	dynamics	1171:1178	arg1	analysis					1180:1187	analysis	1180:1187	analysis	1180:1187	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	3	35	theme	air-assisted	417:428	arg1	technique					438:446	An air-assisted jetting technique	414:446	An air-assisted jetting technique followed by physical crosslinking	414:480	An air-assisted jetting technique followed by physical crosslinking is employed to obtain CHI/GNP microparticles.					
34364561	0	36	theme	high	41:44	arg1	strength					57:64	high mechanical strength	41:64	high mechanical strength	41:64	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	6	37	theme	immunocytochemical	865:882	arg1	characterization					884:899	The morphological and immunocytochemical characterization	843:899	The morphological and immunocytochemical characterization	843:899	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	2	38	dep	cell	379:382	arg1	adhesion					384:391	adhesion	384:391	adhesion	384:391	The aim is to improve the scaffold strength while maintaining its ability to sustain cell adhesion and differentiation.					
34364561	3	39	theme	physical	460:467	arg1	crosslinking					469:480	physical crosslinking	460:480	physical crosslinking	460:480	An air-assisted jetting technique followed by physical crosslinking is employed to obtain CHI/GNP microparticles.					
34364561	7	40	theme	drug	1193:1196	arg1	screening					1198:1206	drug screening	1193:1206	drug screening	1193:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	7	40	theme	drug	1193:1196	arg1	dynamics					1171:1178	networks dynamics	1162:1178	networks dynamics analysis and drug screening	1162:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	4	41	theme	Optical	528:534	arg1	micrographs					584:594	Optical and Field Emission Scanning Electron Microscopy micrographs	528:594	Optical and Field Emission Scanning Electron Microscopy micrographs	528:594	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	7	42	theme	advanced	1121:1128	arg1	models					1151:1156	advanced 3D neuronal in vitro models	1121:1156	advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening	1121:1206	These scaffolds could be employed for the development of advanced 3D neuronal in vitro models for networks dynamics analysis and drug screening.					
34364561	5	43	theme	GNP	679:681	arg1	presence					667:674	The presence	663:674	The presence of GNP	663:681	The presence of GNP turned out to improve the strength of the microparticles while conferring good electrical conductivity and ameliorating their stability in aqueous environment.					
34364561	6	44	theme	morphological	847:859	arg1	characterization					884:899	The morphological and immunocytochemical characterization	843:899	The morphological and immunocytochemical characterization	843:899	The morphological and immunocytochemical characterization, combined with a preliminary electrophysiological analysis, evidenced the effectiveness of the developed composite microparticles as a scaffold for neuron growth.					
34364561	4	45	theme	uniform	605:611	arg1	distribution					613:624	a uniform distribution	603:624	a uniform distribution of GNP within the CHI porous matrix	603:660	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	0	46	theme	strength	57:64	arg1	development					26:36	the development	22:36	the development of high mechanical strength	22:64	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	2	47	theme	scaffold	320:327	arg1	strength					329:336	the scaffold strength	316:336	the scaffold strength	316:336	The aim is to improve the scaffold strength while maintaining its ability to sustain cell adhesion and differentiation.					
34364561	4	48	theme	porous	648:653	arg1	matrix					655:660	the CHI porous matrix	640:660	the CHI porous matrix	640:660	Optical and Field Emission Scanning Electron Microscopy micrographs showed a uniform distribution of GNP within the CHI porous matrix.					
34364561	0	49	theme	mechanical	46:55	arg1	strength					57:64	high mechanical strength	41:64	high mechanical strength	41:64	A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets.					
34364561	1	50	theme	graphite	214:221	arg1	GNP					238:240	GNP	238:240	GNP	238:240	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
34364561	1	50	theme	graphite	214:221	arg1	nanoplatelets					223:235	graphite nanoplatelets	214:235	graphite nanoplatelets (GNP)	214:241	In this work, novel composite microparticles based on chitosan (CHI) and graphite nanoplatelets (GNP) were developed as 3D scaffolds for neuronal cells.					
32438111	4	0	theme	AOP127	607:612	arg1	association					592:602	hydrophobic association	580:602	hydrophobic association of AOP127	580:612	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	9	1	theme	anti-attachment	967:981	arg1	effect					983:988	an anti-attachment effect	964:988	an anti-attachment effect to fibroblasts and biocompatibility in vitro cell studies	964:1046	It also showed an anti-attachment effect to fibroblasts and biocompatibility in vitro cell studies.					
32438111	19	2	theme	dual	2521:2524	arg1	hydrogel					2546:2553	this dual dynamic crosslinked hydrogel	2516:2553	this dual dynamic crosslinked hydrogel	2516:2553	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	14	3	theme	poor	1859:1862	arg1	adhesion					1871:1878	poor tissue adhesion	1859:1878	poor tissue adhesion	1859:1878	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	13	4	theme	postoperative	1651:1663	arg1	prevention					1674:1683	postoperative adhesion prevention	1651:1683	postoperative adhesion prevention	1651:1683	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	12	5	theme	normal	1492:1497	arg1	saline					1499:1504	normal saline	1492:1504	normal saline	1492:1504	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	15	6	theme	dynamic	1953:1959	arg1	hydrogel					1973:1980	a dual dynamic crosslinked hydrogel	1946:1980	a dual dynamic crosslinked hydrogel	1946:1980	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	19	7	theme	potential	2564:2572	arg1	applications					2574:2585	potential applications	2564:2585	potential applications	2564:2585	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	19	7	theme	potential	2564:2572	arg1	barrier					2601:2607	a physical barrier	2590:2607	a physical barrier in clinical practice	2590:2628	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	14	8	theme	due	1852:1854	arg1	trials					1845:1850	clinical trials	1836:1850	clinical trials due to poor tissue adhesion and rapid clearance from injured areas	1836:1917	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	12	9	theme	Pluronic	1509:1516	arg1	hydrogel					1523:1530	Pluronic F127 hydrogel	1509:1530	Pluronic F127 hydrogel	1509:1530	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	17	10	theme	covalent	2220:2227	arg1	bonds					2229:2233	dynamic covalent bonds	2212:2233	dynamic covalent bonds	2212:2233	The introduction of dynamic covalent bonds improves the stability of the hydrogel.					
32438111	2	11	theme	Various	169:175	arg1	strategies					177:186	Various strategies	169:186	Various strategies	169:186	Various strategies have been developed to minimize postoperative adhesion, but the clinical efficacy is still far from satisfactory.					
32438111	9	12	theme	in	1026:1027	arg1	studies					1040:1046	biocompatibility in vitro cell studies	1009:1046	biocompatibility in vitro cell studies	1009:1046	It also showed an anti-attachment effect to fibroblasts and biocompatibility in vitro cell studies.					
32438111	19	13	theme	physical	2592:2599	arg1	applications					2574:2585	potential applications	2564:2585	potential applications	2564:2585	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	19	13	theme	physical	2592:2599	arg1	barrier					2601:2607	a physical barrier	2590:2607	a physical barrier in clinical practice	2590:2628	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	19	14	from	barrier	2601:2607	arg1	practice					2621:2628	clinical practice	2612:2628	clinical practice	2612:2628	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	9	15	theme	cell	1035:1038	arg1	studies					1040:1046	biocompatibility in vitro cell studies	1009:1046	biocompatibility in vitro cell studies	1009:1046	It also showed an anti-attachment effect to fibroblasts and biocompatibility in vitro cell studies.					
32438111	18	16	theme	appropriate	2316:2326	arg1	hemocompatibility					2328:2344	appropriate hemocompatibility	2316:2344	appropriate hemocompatibility	2316:2344	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	10	17	theme	lap-shear	1076:1084	arg1	tests					1086:1090	lap-shear tests	1076:1090	lap-shear tests	1076:1090	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	3	18	theme	dual	323:326	arg1	hydrogel					352:359	a dual dynamically crosslinked hydrogel	321:359	a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier	321:418	Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier.					
32438111	4	19	theme	Pluronic	510:517	arg1	AOP127					525:530	AOP127	525:530	AOP127	525:530	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	4	19	theme	Pluronic	510:517	arg1	F127					519:522	alkoxyamine-terminated Pluronic F127	487:522	alkoxyamine-terminated Pluronic F127 (AOP127)	487:531	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	12	20	theme	hydrogel-treated	1445:1460	arg1	group					1462:1466	the AOP127/OHA hydrogel-treated group	1430:1466	the AOP127/OHA hydrogel-treated group	1430:1466	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	15	21	theme	dynamic	1996:2002	arg1	bonds					2010:2014	dynamic oxime bonds	1996:2014	dynamic oxime bonds	1996:2014	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	13	22	theme	adhesion	1665:1672	arg1	prevention					1674:1683	postoperative adhesion prevention	1651:1683	postoperative adhesion prevention	1651:1683	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	1	23	theme	severe	134:139	arg1	complication					155:166	a severe postoperative complication	132:166	a severe postoperative complication	132:166	Tissue adhesion is a severe postoperative complication.					
32438111	1	23	theme	severe	134:139	arg1	adhesion					120:127	Tissue adhesion	113:127	Tissue adhesion	113:127	Tissue adhesion is a severe postoperative complication.					
32438111	4	24	theme	dynamic	451:457	arg1	oxime					468:472	dynamic chemical oxime	451:472	dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA)	451:566	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	12	25	theme	postoperative	1393:1405	arg1	adhesion					1418:1425	postoperative peritoneal adhesion	1393:1425	postoperative peritoneal adhesion	1393:1425	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	14	26	theme	SIGNIFICANCE	1699:1710	arg1	STATEMENT					1686:1694	STATEMENT	1686:1694	STATEMENT OF SIGNIFICANCE	1686:1710	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	18	27	theme	peritoneal	2479:2488	arg1	adhesions					2490:2498	preventing postoperative peritoneal adhesions	2454:2498	preventing postoperative peritoneal adhesions	2454:2498	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	13	28	theme	physical	1630:1637	arg1	barrier					1639:1645	a physical barrier	1628:1645	a physical barrier for postoperative adhesion prevention	1628:1683	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	3	29	theme	crosslinked	340:350	arg1	hydrogel					352:359	a dual dynamically crosslinked hydrogel	321:359	a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier	321:418	Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier.					
32438111	4	30	theme	oxidized	537:544	arg1	OHA					563:565	OHA	563:565	OHA	563:565	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	4	30	theme	oxidized	537:544	arg1	acid					557:560	oxidized hyaluronic acid	537:560	oxidized hyaluronic acid (OHA)	537:566	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	0	31	theme	anti-adhesion	90:102	arg1	barrier					104:110	a postoperative anti-adhesion barrier	74:110	a postoperative anti-adhesion barrier	74:110	Dual dynamically crosslinked thermosensitive hydrogel with self-fixing as a postoperative anti-adhesion barrier.					
32438111	10	32	theme	moderate	1125:1132	arg1	capacity					1143:1150	a moderate adhesive capacity	1123:1150	a moderate adhesive capacity	1123:1150	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	10	32	theme	moderate	1125:1132	arg1	important					1172:1180	important	1172:1180	important	1172:1180	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	8	33	theme	blood	907:911	arg1	cells					913:917	blood cells	907:917	blood cells	907:917	In addition, it exhibited anti-adhesion to blood cells in blood cell adhesion tests.					
32438111	3	34	theme	physical	375:382	arg1	barrier					412:418	a physical postoperative anti-adhesion barrier	373:418	a physical postoperative anti-adhesion barrier	373:418	Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier.					
32438111	10	35	contain	has	1119:1121	arg2	capacity					1143:1150	a moderate adhesive capacity	1123:1150	a moderate adhesive capacity	1123:1150	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	10	35	contain	has	1119:1121	arg1	hydrogel					1110:1117	the hydrogel	1106:1117	the hydrogel	1106:1117	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	10	35	contain	has	1119:1121	arg2	important					1172:1180	important	1172:1180	important	1172:1180	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	14	36	theme	postoperative	1749:1761	arg1	barriers					1777:1784	numerous postoperative anti-adhesion barriers	1740:1784	numerous postoperative anti-adhesion barriers	1740:1784	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	9	37	dep	in	1026:1027	arg1	vitro					1029:1033	vitro	1029:1033	vitro	1029:1033	It also showed an anti-attachment effect to fibroblasts and biocompatibility in vitro cell studies.					
32438111	11	38	theme	abrasion	1238:1245	arg1	model					1207:1211	A rat model	1201:1211	A rat model of sidewall defect-bowel abrasion	1201:1245	A rat model of sidewall defect-bowel abrasion was established to evaluate the anti-adhesion effect in vivo.					
32438111	6	39	theme	higher	744:749	arg1	stability					751:759	higher stability	744:759	higher stability	744:759	At 37 °C, it shows much higher modulus and higher stability than the Pluronic F127 hydrogel.					
32438111	13	40	theme	self-fixable	1587:1598	arg1	capacity					1600:1607	self-fixable capacity	1587:1607	self-fixable capacity	1587:1607	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	11	41	theme	sidewall	1216:1223	arg1	abrasion					1238:1245	sidewall defect-bowel abrasion	1216:1245	sidewall defect-bowel abrasion	1216:1245	A rat model of sidewall defect-bowel abrasion was established to evaluate the anti-adhesion effect in vivo.					
32438111	8	42	theme	cell	928:931	arg1	tests					942:946	blood cell adhesion tests	922:946	blood cell adhesion tests	922:946	In addition, it exhibited anti-adhesion to blood cells in blood cell adhesion tests.					
32438111	18	43	theme	preventing	2454:2463	arg1	adhesions					2490:2498	preventing postoperative peritoneal adhesions	2454:2498	preventing postoperative peritoneal adhesions	2454:2498	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	19	44	theme	clinical	2612:2619	arg1	practice					2621:2628	clinical practice	2612:2628	clinical practice	2612:2628	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	15	45	link	crosslinked	1961:1971	arg1	hydrogel					1973:1980	a dual dynamic crosslinked hydrogel	1946:1980	a dual dynamic crosslinked hydrogel	1946:1980	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	1	46	theme	Tissue	113:118	arg1	complication					155:166	a severe postoperative complication	132:166	a severe postoperative complication	132:166	Tissue adhesion is a severe postoperative complication.					
32438111	1	46	theme	Tissue	113:118	arg1	adhesion					120:127	Tissue adhesion	113:127	Tissue adhesion	113:127	Tissue adhesion is a severe postoperative complication.					
32438111	12	47	theme	significant	1368:1378	arg1	reduction					1380:1388	a significant reduction	1366:1388	a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group	1366:1466	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	3	48	theme	anti-adhesion	398:410	arg1	barrier					412:418	a physical postoperative anti-adhesion barrier	373:418	a physical postoperative anti-adhesion barrier	373:418	Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier.					
32438111	4	49	theme	hydrophobic	580:590	arg1	association					592:602	hydrophobic association	580:602	hydrophobic association of AOP127	580:612	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	19	50	link	crosslinked	2534:2544	arg1	hydrogel					2546:2553	this dual dynamic crosslinked hydrogel	2516:2553	this dual dynamic crosslinked hydrogel	2516:2553	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	12	51	theme	pathological	1335:1346	arg1	analysis					1348:1355	pathological analysis	1335:1355	pathological analysis	1335:1355	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	14	52	from	areas	1913:1917	arg1	adhesion					1871:1878	poor tissue adhesion	1859:1878	poor tissue adhesion	1859:1878	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	14	52	from	areas	1913:1917	arg1	clearance					1890:1898	rapid clearance	1884:1898	rapid clearance from injured areas	1884:1917	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	14	53	theme	injured	1905:1911	arg1	areas					1913:1917	injured areas	1905:1917	injured areas	1905:1917	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	18	54	theme	fibroblasts	2402:2412	arg1	anti-adhesion					2369:2381	anti-adhesion	2369:2381	anti-adhesion	2369:2381	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	18	54	theme	fibroblasts	2402:2412	arg1	cytocompatibility					2347:2363	cytocompatibility	2347:2363	cytocompatibility	2347:2363	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	18	54	theme	fibroblasts	2402:2412	arg1	hemocompatibility					2328:2344	appropriate hemocompatibility	2316:2344	appropriate hemocompatibility	2316:2344	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	7	55	theme	Hemolytic	794:802	arg1	assays					804:809	Hemolytic assays	794:809	Hemolytic assays	794:809	Hemolytic assays suggested that the hydrogel undergoes low hemolysis.					
32438111	10	56	theme	Macroscopic	1049:1059	arg1	evaluation					1061:1070	Macroscopic evaluation	1049:1070	Macroscopic evaluation	1049:1070	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	18	57	theme	cells	2392:2396	arg1	anti-adhesion					2369:2381	anti-adhesion	2369:2381	anti-adhesion	2369:2381	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	18	57	theme	cells	2392:2396	arg1	cytocompatibility					2347:2363	cytocompatibility	2347:2363	cytocompatibility	2347:2363	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	18	57	theme	cells	2392:2396	arg1	hemocompatibility					2328:2344	appropriate hemocompatibility	2316:2344	appropriate hemocompatibility	2316:2344	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	2	58	theme	postoperative	220:232	arg1	adhesion					234:241	postoperative adhesion	220:241	postoperative adhesion	220:241	Various strategies have been developed to minimize postoperative adhesion, but the clinical efficacy is still far from satisfactory.					
32438111	6	59	theme	Pluronic	770:777	arg1	hydrogel					784:791	the Pluronic F127 hydrogel	766:791	the Pluronic F127 hydrogel	766:791	At 37 °C, it shows much higher modulus and higher stability than the Pluronic F127 hydrogel.					
32438111	16	60	theme	moderate	2101:2108	arg1	capacity					2126:2133	moderate tissue adhesion capacity	2101:2133	moderate tissue adhesion capacity	2101:2133	The hydrogel is temperature-sensitive and demonstrates moderate tissue adhesion capacity, which allows for self-fixation when applied to defects.					
32438111	15	61	theme	crosslinked	1961:1971	arg1	hydrogel					1973:1980	a dual dynamic crosslinked hydrogel	1946:1980	a dual dynamic crosslinked hydrogel	1946:1980	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	14	62	theme	tissue	1864:1869	arg1	adhesion					1871:1878	poor tissue adhesion	1859:1878	poor tissue adhesion	1859:1878	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	12	63	from	reduction	1380:1388	arg1	group					1462:1466	the AOP127/OHA hydrogel-treated group	1430:1466	the AOP127/OHA hydrogel-treated group	1430:1466	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	12	63	from	reduction	1380:1388	arg1	adhesion					1418:1425	postoperative peritoneal adhesion	1393:1425	postoperative peritoneal adhesion	1393:1425	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	15	64	theme	dual	1948:1951	arg1	hydrogel					1973:1980	a dual dynamic crosslinked hydrogel	1946:1980	a dual dynamic crosslinked hydrogel	1946:1980	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	19	65	theme	dynamic	2526:2532	arg1	hydrogel					2546:2553	this dual dynamic crosslinked hydrogel	2516:2553	this dual dynamic crosslinked hydrogel	2516:2553	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	12	66	theme	F127	1518:1521	arg1	hydrogel					1523:1530	Pluronic F127 hydrogel	1509:1530	Pluronic F127 hydrogel	1509:1530	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	13	67	link	crosslinked	1561:1571	arg1	hydrogel					1573:1580	the dual dynamically crosslinked hydrogel	1540:1580	the dual dynamically crosslinked hydrogel with self-fixable capacity	1540:1607	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	13	67	link	crosslinked	1561:1571	arg1	suitable					1616:1623	suitable	1616:1623	suitable	1616:1623	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	17	68	theme	bonds	2229:2233	arg1	introduction					2196:2207	The introduction	2192:2207	The introduction of dynamic covalent bonds	2192:2233	The introduction of dynamic covalent bonds improves the stability of the hydrogel.					
32438111	9	69	theme	biocompatibility	1009:1024	arg1	studies					1040:1046	biocompatibility in vitro cell studies	1009:1046	biocompatibility in vitro cell studies	1009:1046	It also showed an anti-attachment effect to fibroblasts and biocompatibility in vitro cell studies.					
32438111	5	70	theme	temperature	676:686	arg1	sensitivity					688:698	temperature sensitivity	676:698	temperature sensitivity	676:698	Rheological analysis demonstrated that the hydrogel exhibits temperature sensitivity.					
32438111	3	71	link	crosslinked	340:350	arg1	hydrogel					352:359	a dual dynamically crosslinked hydrogel	321:359	a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier	321:418	Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier.					
32438111	14	72	theme	barriers	1777:1784	arg1	development					1725:1735	the development	1721:1735	the development of numerous postoperative anti-adhesion barriers	1721:1784	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	13	73	with	hydrogel	1573:1580	arg1	capacity					1600:1607	self-fixable capacity	1587:1607	self-fixable capacity	1587:1607	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	4	74	theme	alkoxyamine-terminated	487:508	arg1	AOP127					525:530	AOP127	525:530	AOP127	525:530	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	4	74	theme	alkoxyamine-terminated	487:508	arg1	F127					519:522	alkoxyamine-terminated Pluronic F127	487:522	alkoxyamine-terminated Pluronic F127 (AOP127)	487:531	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	15	75	theme	oxime	2004:2008	arg1	bonds					2010:2014	dynamic oxime bonds	1996:2014	dynamic oxime bonds	1996:2014	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	14	76	theme	clinical	1836:1843	arg1	trials					1845:1850	clinical trials	1836:1850	clinical trials due to poor tissue adhesion and rapid clearance from injured areas	1836:1917	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	18	77	theme	blood	2386:2390	arg1	cells					2392:2396	blood cells	2386:2396	blood cells	2386:2396	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	17	78	theme	hydrogel	2265:2272	arg1	stability					2248:2256	the stability	2244:2256	the stability of the hydrogel	2244:2272	The introduction of dynamic covalent bonds improves the stability of the hydrogel.					
32438111	2	79	theme	clinical	252:259	arg1	efficacy					261:268	the clinical efficacy	248:268	the clinical efficacy	248:268	Various strategies have been developed to minimize postoperative adhesion, but the clinical efficacy is still far from satisfactory.					
32438111	2	79	theme	clinical	252:259	arg1	satisfactory					288:299	satisfactory	288:299	satisfactory	288:299	Various strategies have been developed to minimize postoperative adhesion, but the clinical efficacy is still far from satisfactory.					
32438111	4	80	theme	chemical	459:466	arg1	oxime					468:472	dynamic chemical oxime	451:472	dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA)	451:566	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	12	81	theme	AOP127/OHA	1434:1443	arg1	group					1462:1466	the AOP127/OHA hydrogel-treated group	1430:1466	the AOP127/OHA hydrogel-treated group	1430:1466	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	1	82	theme	postoperative	141:153	arg1	complication					155:166	a severe postoperative complication	132:166	a severe postoperative complication	132:166	Tissue adhesion is a severe postoperative complication.					
32438111	1	82	theme	postoperative	141:153	arg1	adhesion					120:127	Tissue adhesion	113:127	Tissue adhesion	113:127	Tissue adhesion is a severe postoperative complication.					
32438111	0	83	theme	postoperative	76:88	arg1	barrier					104:110	a postoperative anti-adhesion barrier	74:110	a postoperative anti-adhesion barrier	74:110	Dual dynamically crosslinked thermosensitive hydrogel with self-fixing as a postoperative anti-adhesion barrier.					
32438111	14	84	theme	anti-adhesion	1793:1805	arg1	efficacy					1807:1814	their anti-adhesion efficacy	1787:1814	their anti-adhesion efficacy	1787:1814	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	4	85	theme	hyaluronic	546:555	arg1	OHA					563:565	OHA	563:565	OHA	563:565	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	4	85	theme	hyaluronic	546:555	arg1	acid					557:560	oxidized hyaluronic acid	537:560	oxidized hyaluronic acid (OHA)	537:566	The hydrogel was generated by dynamic chemical oxime bonding from alkoxyamine-terminated Pluronic F127 (AOP127) and oxidized hyaluronic acid (OHA), as well as hydrophobic association of AOP127.					
32438111	12	86	theme	peritoneal	1407:1416	arg1	adhesion					1418:1425	postoperative peritoneal adhesion	1393:1425	postoperative peritoneal adhesion	1393:1425	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	10	87	theme	adhesive	1134:1141	arg1	capacity					1143:1150	a moderate adhesive capacity	1123:1150	a moderate adhesive capacity	1123:1150	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	10	87	theme	adhesive	1134:1141	arg1	important					1172:1180	important	1172:1180	important	1172:1180	Macroscopic evaluation and lap-shear tests revealed that the hydrogel has a moderate adhesive capacity to tissue, which is important for self-fixation.					
32438111	19	88	contain	have	2559:2562	arg2	barrier					2601:2607	a physical barrier	2590:2607	a physical barrier in clinical practice	2590:2628	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	19	88	contain	have	2559:2562	arg2	applications					2574:2585	potential applications	2564:2585	potential applications	2564:2585	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	19	88	contain	have	2559:2562	arg1	hydrogel					2546:2553	this dual dynamic crosslinked hydrogel	2516:2553	this dual dynamic crosslinked hydrogel	2516:2553	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
32438111	15	89	theme	hydrophobic	2020:2030	arg1	interactions					2032:2043	hydrophobic interactions	2020:2043	hydrophobic interactions	2020:2043	Herein, we have developed a dual dynamic crosslinked hydrogel, generated by dynamic oxime bonds and hydrophobic interactions.					
32438111	17	90	theme	dynamic	2212:2218	arg1	bonds					2229:2233	dynamic covalent bonds	2212:2233	dynamic covalent bonds	2212:2233	The introduction of dynamic covalent bonds improves the stability of the hydrogel.					
32438111	14	91	theme	anti-adhesion	1763:1775	arg1	barriers					1777:1784	numerous postoperative anti-adhesion barriers	1740:1784	numerous postoperative anti-adhesion barriers	1740:1784	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	11	92	theme	defect-bowel	1225:1236	arg1	abrasion					1238:1245	sidewall defect-bowel abrasion	1216:1245	sidewall defect-bowel abrasion	1216:1245	A rat model of sidewall defect-bowel abrasion was established to evaluate the anti-adhesion effect in vivo.					
32438111	3	93	theme	postoperative	384:396	arg1	barrier					412:418	a physical postoperative anti-adhesion barrier	373:418	a physical postoperative anti-adhesion barrier	373:418	Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier.					
32438111	16	94	theme	adhesion	2117:2124	arg1	capacity					2126:2133	moderate tissue adhesion capacity	2101:2133	moderate tissue adhesion capacity	2101:2133	The hydrogel is temperature-sensitive and demonstrates moderate tissue adhesion capacity, which allows for self-fixation when applied to defects.					
32438111	14	95	theme	numerous	1740:1747	arg1	barriers					1777:1784	numerous postoperative anti-adhesion barriers	1740:1784	numerous postoperative anti-adhesion barriers	1740:1784	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	18	96	theme	postoperative	2465:2477	arg1	adhesions					2490:2498	preventing postoperative peritoneal adhesions	2454:2498	preventing postoperative peritoneal adhesions	2454:2498	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	8	97	theme	blood	922:926	arg1	tests					942:946	blood cell adhesion tests	922:946	blood cell adhesion tests	922:946	In addition, it exhibited anti-adhesion to blood cells in blood cell adhesion tests.					
32438111	6	98	theme	higher	725:730	arg1	modulus					732:738	much higher modulus	720:738	much higher modulus	720:738	At 37 °C, it shows much higher modulus and higher stability than the Pluronic F127 hydrogel.					
32438111	0	99	theme	thermosensitive	29:43	arg1	hydrogel					45:52	thermosensitive hydrogel	29:52	thermosensitive hydrogel	29:52	Dual dynamically crosslinked thermosensitive hydrogel with self-fixing as a postoperative anti-adhesion barrier.					
32438111	5	100	theme	Rheological	615:625	arg1	analysis					627:634	Rheological analysis	615:634	Rheological analysis	615:634	Rheological analysis demonstrated that the hydrogel exhibits temperature sensitivity.					
32438111	8	101	theme	adhesion	933:940	arg1	tests					942:946	blood cell adhesion tests	922:946	blood cell adhesion tests	922:946	In addition, it exhibited anti-adhesion to blood cells in blood cell adhesion tests.					
32438111	13	102	theme	crosslinked	1561:1571	arg1	hydrogel					1573:1580	the dual dynamically crosslinked hydrogel	1540:1580	the dual dynamically crosslinked hydrogel with self-fixable capacity	1540:1607	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	13	102	theme	crosslinked	1561:1571	arg1	suitable					1616:1623	suitable	1616:1623	suitable	1616:1623	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	7	103	theme	low	849:851	arg1	hemolysis					853:861	low hemolysis	849:861	low hemolysis	849:861	Hemolytic assays suggested that the hydrogel undergoes low hemolysis.					
32438111	11	104	theme	anti-adhesion	1279:1291	arg1	effect					1293:1298	the anti-adhesion effect	1275:1298	the anti-adhesion effect	1275:1298	A rat model of sidewall defect-bowel abrasion was established to evaluate the anti-adhesion effect in vivo.					
32438111	18	105	dep	Moreover	2275:2282	arg1	only					2302:2305	only	2302:2305	only	2302:2305	Moreover, the hydrogel not only displays appropriate hemocompatibility, cytocompatibility and anti-adhesion of blood cells and fibroblasts, but it also effectively contributes to preventing postoperative peritoneal adhesions in vivo.					
32438111	11	106	theme	rat	1203:1205	arg1	model					1207:1211	A rat model	1201:1211	A rat model of sidewall defect-bowel abrasion	1201:1245	A rat model of sidewall defect-bowel abrasion was established to evaluate the anti-adhesion effect in vivo.					
32438111	12	107	theme	gross	1313:1317	arg1	observation					1319:1329	The gross observation	1309:1329	The gross observation	1309:1329	The gross observation and pathological analysis revealed a significant reduction in postoperative peritoneal adhesion in the AOP127/OHA hydrogel-treated group than those treated with normal saline or Pluronic F127 hydrogel.					
32438111	6	108	theme	F127	779:782	arg1	hydrogel					784:791	the Pluronic F127 hydrogel	766:791	the Pluronic F127 hydrogel	766:791	At 37 °C, it shows much higher modulus and higher stability than the Pluronic F127 hydrogel.					
32438111	13	109	theme	dual	1544:1547	arg1	hydrogel					1573:1580	the dual dynamically crosslinked hydrogel	1540:1580	the dual dynamically crosslinked hydrogel with self-fixable capacity	1540:1607	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	13	109	theme	dual	1544:1547	arg1	suitable					1616:1623	suitable	1616:1623	suitable	1616:1623	Hence, the dual dynamically crosslinked hydrogel with self-fixable capacity may be suitable as a physical barrier for postoperative adhesion prevention.					
32438111	14	110	theme	rapid	1884:1888	arg1	clearance					1890:1898	rapid clearance	1884:1898	rapid clearance from injured areas	1884:1917	STATEMENT OF SIGNIFICANCE: Despite the development of numerous postoperative anti-adhesion barriers, their anti-adhesion efficacy is still limited in clinical trials due to poor tissue adhesion and rapid clearance from injured areas.					
32438111	16	111	theme	tissue	2110:2115	arg1	capacity					2126:2133	moderate tissue adhesion capacity	2101:2133	moderate tissue adhesion capacity	2101:2133	The hydrogel is temperature-sensitive and demonstrates moderate tissue adhesion capacity, which allows for self-fixation when applied to defects.					
32438111	19	112	theme	crosslinked	2534:2544	arg1	hydrogel					2546:2553	this dual dynamic crosslinked hydrogel	2516:2553	this dual dynamic crosslinked hydrogel	2516:2553	Hence, this dual dynamic crosslinked hydrogel may have potential applications as a physical barrier in clinical practice.					
34453701	0	0	theme	fish	85:88	arg1	gut					90:92	fish gut	85:92	fish gut	85:92	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	4	1	theme	13C-NMR	485:491	arg1	spectroscopy					493:504	13C-NMR spectroscopy	485:504	13C-NMR spectroscopy	485:504	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	7	2	theme	linear	1057:1062	arg1	first					1077:1081	first	1077:1081	first	1077:1081	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	2	theme	linear	1057:1062	arg1	levan					1064:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	1	3	theme	Gram-positive	157:169	arg1	strain					181:186	potentially probiotic Gram-positive bacterial strain	135:186	potentially probiotic Gram-positive bacterial strain	135:186	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	6	4	theme	optimized	912:920	arg1	pH					974:975	pH	974:975	pH	974:975	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	4	theme	optimized	912:920	arg1	conditions					962:971	optimized culturing parameters including aeration conditions	912:971	optimized culturing parameters including aeration conditions	912:971	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	4	theme	optimized	912:920	arg1	temperature					978:988	temperature	978:988	temperature	978:988	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	4	theme	optimized	912:920	arg1	concentration					1004:1016	substrate concentration	994:1016	substrate concentration	994:1016	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	5	5	theme	levan	732:736	arg1	groups					722:727	two groups	718:727	two groups of levan	718:736	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	5	theme	levan	732:736	arg1	levan					732:736	levan	732:736	levan	732:736	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	4	6	theme	methylation	507:517	arg1	analysis					519:526	methylation analysis	507:526	methylation analysis	507:526	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	7	7	from	gut	1147:1149	arg1	isolate					1129:1135	a L. reuteri isolate	1116:1135	a L. reuteri isolate from fish gut	1116:1149	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	1	8	theme	bacterial	171:179	arg1	strain					181:186	potentially probiotic Gram-positive bacterial strain	135:186	potentially probiotic Gram-positive bacterial strain	135:186	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	7	9	theme	molecular	1040:1048	arg1	first					1077:1081	first	1077:1081	first	1077:1081	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	9	theme	molecular	1040:1048	arg1	levan					1064:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	4	10	theme	linear	588:593	arg1	polymer					601:607	a linear levan polymer	586:607	a linear levan polymer with fructose	586:621	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	4	10	theme	linear	588:593	arg1	EPS					564:566	the EPS	560:566	the EPS	560:566	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	7	11	theme	L.	1118:1119	arg1	isolate					1129:1135	a L. reuteri isolate	1116:1135	a L. reuteri isolate from fish gut	1116:1149	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	12	theme	weight	1050:1055	arg1	first					1077:1081	first	1077:1081	first	1077:1081	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	12	theme	weight	1050:1055	arg1	levan					1064:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	2	13	dep	Lactobacillus	325:337	arg1	reuteri					339:345	reuteri	339:345	reuteri	339:345	The isolate, designated as FW2, was identified as Lactobacillus reuteri through 16S rRNA gene sequencing and phylogenetic analysis.					
34453701	5	14	theme	high	793:796	arg1	MW					798:799	high MW	793:799	high MW (4.6 × 106 Da)	793:814	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	14	theme	high	793:796	arg1	4.6 × 106 Da					802:813	4.6 × 106 Da	802:813	4.6 × 106 Da	802:813	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	7	15	theme	obtained	1023:1030	arg1	first					1077:1081	first	1077:1081	first	1077:1081	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	15	theme	obtained	1023:1030	arg1	levan					1064:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	8	16	theme	new	1271:1273	arg1	combination					1295:1305	a new promising synbiotic combination	1269:1305	a new promising synbiotic combination	1269:1305	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	8	17	theme	molecular	1160:1168	arg1	levan					1187:1191	Bimodal molecular weight prebiotic levan	1152:1191	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain	1152:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	3	18	theme	fructan-type	429:440	arg1	EPS					442:444	fructan-type EPS	429:444	fructan-type EPS using sucrose as a substrate	429:473	This isolate produces fructan-type EPS using sucrose as a substrate.					
34453701	7	19	theme	bimodal	1032:1038	arg1	first					1077:1081	first	1077:1081	first	1077:1081	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	19	theme	bimodal	1032:1038	arg1	levan					1064:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	6	20	theme	substrate	994:1002	arg1	conditions					962:971	optimized culturing parameters including aeration conditions	912:971	optimized culturing parameters including aeration conditions	912:971	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	20	theme	substrate	994:1002	arg1	concentration					1004:1016	substrate concentration	994:1016	substrate concentration	994:1016	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	8	21	theme	synbiotic	1285:1293	arg1	combination					1295:1305	a new promising synbiotic combination	1269:1305	a new promising synbiotic combination	1269:1305	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	8	22	from	use	1311:1313	arg1	culture					1323:1329	aqua culture	1318:1329	aqua culture	1318:1329	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	2	23	theme	gene	364:367	arg1	sequencing					369:378	rRNA gene sequencing	359:378	rRNA gene sequencing	359:378	The isolate, designated as FW2, was identified as Lactobacillus reuteri through 16S rRNA gene sequencing and phylogenetic analysis.					
34453701	0	24	from	gut	90:92	arg1	isolate					72:78	a Lactobacillus reuteri isolate	48:78	a Lactobacillus reuteri isolate from fish gut	48:92	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	8	25	theme	promising	1275:1283	arg1	combination					1295:1305	a new promising synbiotic combination	1269:1305	a new promising synbiotic combination	1269:1305	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	2	26	theme	rRNA	359:362	arg1	sequencing					369:378	rRNA gene sequencing	359:378	rRNA gene sequencing	359:378	The isolate, designated as FW2, was identified as Lactobacillus reuteri through 16S rRNA gene sequencing and phylogenetic analysis.					
34453701	0	27	theme	molecular	22:30	arg1	levan					39:43	bimodal molecular weight levan	14:43	bimodal molecular weight levan	14:43	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	2	28	theme	phylogenetic	384:395	arg1	analysis					397:404	phylogenetic analysis	384:404	phylogenetic analysis	384:404	The isolate, designated as FW2, was identified as Lactobacillus reuteri through 16S rRNA gene sequencing and phylogenetic analysis.					
34453701	0	29	theme	bimodal	14:20	arg1	levan					39:43	bimodal molecular weight levan	14:43	bimodal molecular weight levan	14:43	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	5	30	theme	molecular	682:690	arg1	MW					700:701	MW	700:701	MW	700:701	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	30	theme	molecular	682:690	arg1	weight					692:697	molecular weight	682:697	molecular weight (MW) distribution	682:715	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	8	31	theme	producing	1238:1246	arg1	strain					1248:1253	the producing strain	1234:1253	the producing strain	1234:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	8	32	theme	weight	1170:1175	arg1	levan					1187:1191	Bimodal molecular weight prebiotic levan	1152:1191	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain	1152:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	0	33	theme	levan	39:43	arg1	Production					0:9	Production	0:9	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.	0:93	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	5	34	theme	weight	692:697	arg1	distribution					704:715	molecular weight (MW) distribution	682:715	molecular weight (MW) distribution	682:715	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	0	35	dep	a Lactobacillus	48:62	arg1	reuteri					64:70	reuteri	64:70	reuteri	64:70	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	8	36	theme	Bimodal	1152:1158	arg1	levan					1187:1191	Bimodal molecular weight prebiotic levan	1152:1191	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain	1152:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	1	37	theme	Tor	212:214	arg1	gut					226:228	fish (Tor putitora) gut	206:228	fish (Tor putitora) gut	206:228	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	0	38	theme	weight	32:37	arg1	levan					39:43	bimodal molecular weight levan	14:43	bimodal molecular weight levan	14:43	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	1	39	theme	putitora	216:223	arg1	gut					226:228	fish (Tor putitora) gut	206:228	fish (Tor putitora) gut	206:228	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	0	40	theme	a Lactobacillus	48:62	arg1	isolate					72:78	a Lactobacillus reuteri isolate	48:78	a Lactobacillus reuteri isolate from fish gut	48:92	Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.					
34453701	8	41	theme	prebiotic	1177:1185	arg1	levan					1187:1191	Bimodal molecular weight prebiotic levan	1152:1191	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain	1152:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	1	42	attach	isolated	192:199	arg2	exopolysaccharide					98:114	An exopolysaccharide	95:114	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain	95:186	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	1	42	attach	isolated	192:199	arg1	gut					226:228	fish (Tor putitora) gut	206:228	fish (Tor putitora) gut	206:228	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	1	42	attach	isolated	192:199	arg2	EPS					117:119	EPS	117:119	EPS	117:119	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	4	43	with	polymer	601:607	arg1	fructose					614:621	fructose	614:621	fructose	614:621	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	8	44	theme	aqua	1318:1321	arg1	culture					1323:1329	aqua culture	1318:1329	aqua culture	1318:1329	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	7	45	theme	type	1090:1093	arg1	first					1077:1081	first	1077:1081	first	1077:1081	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	7	45	theme	type	1090:1093	arg1	levan					1064:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan	1019:1068	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	4	46	theme	monosaccharide	532:545	arg1	composition					547:557	monosaccharide composition	532:557	monosaccharide composition	532:557	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	5	47	contain	having	830:835	arg2	MW					848:849	much lower MW	837:849	much lower MW (1.2 × 104 Da)	837:864	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	47	contain	having	830:835	arg1	other					824:828	other	824:828	other	824:828	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	47	contain	having	830:835	arg2	1.2 × 104 Da					852:863	1.2 × 104 Da	852:863	1.2 × 104 Da	852:863	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	48	theme	lower	842:846	arg1	1.2 × 104 Da					852:863	1.2 × 104 Da	852:863	1.2 × 104 Da	852:863	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	5	48	theme	lower	842:846	arg1	MW					848:849	much lower MW	837:849	much lower MW (1.2 × 104 Da)	837:864	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	7	49	dep	L.	1118:1119	arg1	reuteri					1121:1127	reuteri	1121:1127	reuteri	1121:1127	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	5	50	theme	isolate	771:777	arg1	FW2					779:781	the isolate FW2	767:781	the isolate FW2	767:781	Based on molecular weight (MW) distribution, two groups of levan were found to be produced by the isolate FW2: one with high MW (4.6 × 106 Da) and the other having much lower MW (1.2 × 104 Da).					
34453701	8	51	theme	probiotic	1211:1219	arg1	potential					1221:1229	the probiotic potential	1207:1229	the probiotic potential of the producing strain	1207:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	8	52	theme	strain	1248:1253	arg1	potential					1221:1229	the probiotic potential	1207:1229	the probiotic potential of the producing strain	1207:1253	Bimodal molecular weight prebiotic levan together with the probiotic potential of the producing strain would provide a new promising synbiotic combination for use in aqua culture.					
34453701	4	53	theme	levan	595:599	arg1	polymer					601:607	a linear levan polymer	586:607	a linear levan polymer with fructose	586:621	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	4	53	theme	levan	595:599	arg1	EPS					564:566	the EPS	560:566	the EPS	560:566	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	4	54	attach	linked	643:648	arg3	2 → 6					656:660	2 → 6	656:660	2 → 6	656:660	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	4	54	attach	linked	643:648	arg3	linkages					663:670	β(2 → 6) linkages	654:670	β(2 → 6) linkages	654:670	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	1	55	theme	fish	206:209	arg1	gut					226:228	fish (Tor putitora) gut	206:228	fish (Tor putitora) gut	206:228	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	6	56	theme	parameters	932:941	arg1	pH					974:975	pH	974:975	pH	974:975	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	56	theme	parameters	932:941	arg1	conditions					962:971	optimized culturing parameters including aeration conditions	912:971	optimized culturing parameters including aeration conditions	912:971	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	56	theme	parameters	932:941	arg1	temperature					978:988	temperature	978:988	temperature	978:988	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	56	theme	parameters	932:941	arg1	concentration					1004:1016	substrate concentration	994:1016	substrate concentration	994:1016	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	7	57	theme	fish	1142:1145	arg1	gut					1147:1149	fish gut	1142:1149	fish gut	1142:1149	The obtained bimodal molecular weight linear levan is the first of its type to be synthesized by a L. reuteri isolate from fish gut.					
34453701	4	58	theme	main	626:629	arg1	constituent					631:641	main constituent	626:641	main constituent linked via β(2 → 6) linkages	626:670	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	4	58	theme	main	626:629	arg1	EPS					564:566	the EPS	560:566	the EPS	560:566	Based on 13C-NMR spectroscopy, methylation analysis and monosaccharide composition, the EPS was identified as a linear levan polymer with fructose as main constituent linked via β(2 → 6) linkages.					
34453701	1	59	theme	probiotic	147:155	arg1	strain					181:186	potentially probiotic Gram-positive bacterial strain	135:186	potentially probiotic Gram-positive bacterial strain	135:186	An exopolysaccharide (EPS) synthesizing potentially probiotic Gram-positive bacterial strain was isolated from fish (Tor putitora) gut, and its EPS was structurally characterized.					
34453701	6	60	theme	culturing	922:930	arg1	parameters					932:941	culturing parameters	922:941	optimized culturing parameters including aeration conditions	912:971	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
34453701	6	60	theme	culturing	922:930	arg1	aeration					953:960	aeration	953:960	aeration	953:960	The isolate yielded about 14 g/L levan under optimized culturing parameters including aeration conditions, pH, temperature and substrate concentration.					
32713255	10	0	theme	Bayesian	1052:1059	arg1	Regularization					1061:1074	Bayesian Regularization	1052:1074	Bayesian Regularization	1052:1074	A multilayer feed-forward algorithm was performed for ANN, i.e., Levenberg-Marquardt, Bayesian Regularization, and Scaled Conjugate Gradient.					
32713255	5	1	theme	fungus	554:559	arg1	9331					590:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	8	2	theme	central	826:832	arg1	design					844:849	The central composite design	822:849	The central composite design with RSM	822:858	The central composite design with RSM was used for the optimization of the process parameters.					
32713255	4	3	theme	industrial	437:446	arg1	application					448:458	their industrial application	431:458	their industrial application	431:458	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	4	4	theme	chitinase	407:415	arg1	stability					394:402	characteristics thermal stability	370:402	characteristics thermal stability of chitinase	370:415	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	14	5	theme	optimization	1541:1552	arg1	results					1554:1560	better optimization results	1534:1560	better optimization results	1534:1560	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	6	6	theme	surface	654:660	arg1	methodology					662:672	response surface methodology	645:672	response surface methodology (RSM)	645:678	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	6	theme	surface	654:660	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	6	theme	surface	654:660	arg1	RSM					675:677	RSM	675:677	RSM	675:677	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	14	7	theme	better	1534:1539	arg1	results					1554:1560	better optimization results	1534:1560	better optimization results	1534:1560	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	10	8	theme	multilayer	968:977	arg1	algorithm					992:1000	A multilayer feed-forward algorithm	966:1000	A multilayer feed-forward algorithm	966:1000	A multilayer feed-forward algorithm was performed for ANN, i.e., Levenberg-Marquardt, Bayesian Regularization, and Scaled Conjugate Gradient.					
32713255	6	9	theme	response	645:652	arg1	methodology					662:672	response surface methodology	645:672	response surface methodology (RSM)	645:678	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	9	theme	response	645:652	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	9	theme	response	645:652	arg1	RSM					675:677	RSM	675:677	RSM	675:677	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	10	10	theme	Conjugate	1088:1096	arg1	Gradient					1098:1105	Scaled Conjugate Gradient	1081:1105	Scaled Conjugate Gradient	1081:1105	A multilayer feed-forward algorithm was performed for ANN, i.e., Levenberg-Marquardt, Bayesian Regularization, and Scaled Conjugate Gradient.					
32713255	8	11	theme	process	897:903	arg1	parameters					905:914	the process parameters	893:914	the process parameters	893:914	The central composite design with RSM was used for the optimization of the process parameters.					
32713255	11	12	dep	88.38 U/L	1225:1233	arg1	i.e.					1219:1222	i.e.	1219:1222	i.e.	1219:1222	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	10	13	theme	Scaled	1081:1086	arg1	Gradient					1098:1105	Scaled Conjugate Gradient	1081:1105	Scaled Conjugate Gradient	1081:1105	A multilayer feed-forward algorithm was performed for ANN, i.e., Levenberg-Marquardt, Bayesian Regularization, and Scaled Conjugate Gradient.					
32713255	8	14	used	used	864:867	arg2	design					844:849	The central composite design	822:849	The central composite design with RSM	822:858	The central composite design with RSM was used for the optimization of the process parameters.					
32713255	13	15	theme	experimental	1429:1440	arg1	analysis					1442:1449	experimental analysis	1429:1449	experimental analysis	1429:1449	The validation study suggested that the highest activity of chitinase as predicted by ANN is in line with experimental analysis.					
32713255	11	16	theme	chitinase	1145:1153	arg1	activity					1155:1162	higher chitinase activity	1138:1162	higher chitinase activity	1138:1162	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	5	17	theme	parameter	500:508	arg1	optimization					510:521	parameter optimization	500:521	parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331	500:593	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	6	18	theme	optimization	612:623	arg1	methodology					662:672	response surface methodology	645:672	response surface methodology (RSM)	645:678	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	18	theme	optimization	612:623	arg1	network					699:705	artificial neural network	681:705	artificial neural network (ANN)	681:711	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	18	theme	optimization	612:623	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	18	theme	optimization	612:623	arg1	algorithm					725:733	genetic algorithm	717:733	genetic algorithm (GA)	717:738	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	4	19	theme	characteristics	370:384	arg1	stability					394:402	characteristics thermal stability	370:402	characteristics thermal stability of chitinase	370:415	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	11	20	dep	ANN	1112:1114	arg1	values					1126:1131	predicted values	1116:1131	The ANN predicted values	1108:1131	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	14	21	theme	RSM	1585:1587	arg1	study					1589:1593	the GA and RSM study	1574:1593	the GA and RSM study	1574:1593	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	0	22	theme	Thermomyces	72:82	arg1	sp					84:85	Thermomyces sp	72:85	Thermomyces sp	72:85	Optimization of process parameters for improved chitinase activity from Thermomyces sp.					
32713255	8	23	with	design	844:849	arg1	RSM					856:858	RSM	856:858	RSM	856:858	The central composite design with RSM was used for the optimization of the process parameters.					
32713255	14	24	theme	approaches	1498:1507	arg1	comparison					1456:1465	The comparison	1452:1465	The comparison of three different statistical approaches	1452:1507	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	14	25	theme	statistical	1486:1496	arg1	approaches					1498:1507	three different statistical approaches	1470:1507	three different statistical approaches	1470:1507	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	4	26	with	yield	359:363	arg1	stability					394:402	characteristics thermal stability	370:402	characteristics thermal stability of chitinase	370:415	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	2	27	theme	breaking	179:186	arg1	down					188:191	the breaking down	175:191	the breaking down of chitin to N-acetyl-glucosamine units linked through (1-4)-glycosidic bond	175:268	Chitinase is responsible for the breaking down of chitin to N-acetyl-glucosamine units linked through (1-4)-glycosidic bond.					
32713255	0	28	theme	parameters	24:33	arg1	Optimization					0:11	Optimization	0:11	Optimization of process parameters for improved chitinase activity from Thermomyces sp.	0:86	Optimization of process parameters for improved chitinase activity from Thermomyces sp.					
32713255	13	29	theme	highest	1363:1369	arg1	activity					1371:1378	the highest activity	1359:1378	the highest activity of chitinase as predicted by ANN	1359:1411	The validation study suggested that the highest activity of chitinase as predicted by ANN is in line with experimental analysis.					
32713255	4	30	theme	thermal	386:392	arg1	stability					394:402	characteristics thermal stability	370:402	characteristics thermal stability of chitinase	370:415	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	1	31	theme	artificial	97:106	arg1	network					115:121	artificial neural network	97:121	artificial neural network	97:121	by using artificial neural network and genetic algorithm.					
32713255	5	32	theme	present	476:482	arg1	work					484:487	the present work	472:487	the present work	472:487	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	12	33	theme	predicted	1240:1248	arg1	activity					1260:1267	The predicted chitinase activity	1236:1267	The predicted chitinase activity	1236:1267	The predicted chitinase activity was also closer to the observed data at these levels.					
32713255	12	33	theme	predicted	1240:1248	arg1	closer					1278:1283	closer	1278:1283	closer	1278:1283	The predicted chitinase activity was also closer to the observed data at these levels.					
32713255	0	34	theme	process	16:22	arg1	parameters					24:33	process parameters	16:33	process parameters	16:33	Optimization of process parameters for improved chitinase activity from Thermomyces sp.					
32713255	14	35	theme	GA	1578:1579	arg1	study					1589:1593	the GA and RSM study	1574:1593	the GA and RSM study	1574:1593	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	1	36	theme	neural	108:113	arg1	network					115:121	artificial neural network	97:121	artificial neural network	97:121	by using artificial neural network and genetic algorithm.					
32713255	7	37	theme	inoculum	807:814	arg1	size					816:819	inoculum size	807:819	inoculum size	807:819	The parameters under study were incubation time, pH and inoculum size.					
32713255	13	38	theme	chitinase	1383:1391	arg1	activity					1371:1378	the highest activity	1359:1378	the highest activity of chitinase as predicted by ANN	1359:1411	The validation study suggested that the highest activity of chitinase as predicted by ANN is in line with experimental analysis.					
32713255	0	39	theme	improved	39:46	arg1	activity					58:65	improved chitinase activity	39:65	improved chitinase activity from Thermomyces sp	39:85	Optimization of process parameters for improved chitinase activity from Thermomyces sp.					
32713255	6	40	theme	genetic	717:723	arg1	GA					736:737	GA	736:737	GA	736:737	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	40	theme	genetic	717:723	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	40	theme	genetic	717:723	arg1	algorithm					725:733	genetic algorithm	717:733	genetic algorithm (GA)	717:738	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	8	41	theme	parameters	905:914	arg1	optimization					877:888	the optimization	873:888	the optimization of the process parameters	873:914	The central composite design with RSM was used for the optimization of the process parameters.					
32713255	0	42	from	sp	84:85	arg1	activity					58:65	improved chitinase activity	39:65	improved chitinase activity from Thermomyces sp	39:85	Optimization of process parameters for improved chitinase activity from Thermomyces sp.					
32713255	6	43	theme	neural	692:697	arg1	ANN					708:710	ANN	708:710	ANN	708:710	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	43	theme	neural	692:697	arg1	network					699:705	artificial neural network	681:705	artificial neural network (ANN)	681:711	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	43	theme	neural	692:697	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	44	theme	different	602:610	arg1	methodology					662:672	response surface methodology	645:672	response surface methodology (RSM)	645:678	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	44	theme	different	602:610	arg1	network					699:705	artificial neural network	681:705	artificial neural network (ANN)	681:711	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	44	theme	different	602:610	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	44	theme	different	602:610	arg1	algorithm					725:733	genetic algorithm	717:733	genetic algorithm (GA)	717:738	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	13	45	theme	validation	1327:1336	arg1	study					1338:1342	The validation study	1323:1342	The validation study	1323:1342	The validation study suggested that the highest activity of chitinase as predicted by ANN is in line with experimental analysis.					
32713255	11	46	theme	RSM-predicted	1197:1209	arg1	values					1211:1216	RSM-predicted values	1197:1216	RSM-predicted values	1197:1216	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	5	47	theme	Thermomyces	561:571	arg1	9331					590:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	12	48	theme	chitinase	1250:1258	arg1	activity					1260:1267	The predicted chitinase activity	1236:1267	The predicted chitinase activity	1236:1267	The predicted chitinase activity was also closer to the observed data at these levels.					
32713255	12	48	theme	chitinase	1250:1258	arg1	closer					1278:1283	closer	1278:1283	closer	1278:1283	The predicted chitinase activity was also closer to the observed data at these levels.					
32713255	7	49	theme	incubation	783:792	arg1	time					794:797	incubation time	783:797	incubation time	783:797	The parameters under study were incubation time, pH and inoculum size.					
32713255	7	49	theme	incubation	783:792	arg1	parameters					755:764	The parameters	751:764	The parameters under study	751:776	The parameters under study were incubation time, pH and inoculum size.					
32713255	5	50	theme	lanuginosus	573:583	arg1	9331					590:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	0	51	theme	chitinase	48:56	arg1	activity					58:65	improved chitinase activity	39:65	improved chitinase activity from Thermomyces sp	39:85	Optimization of process parameters for improved chitinase activity from Thermomyces sp.					
32713255	3	52	theme	waste	315:319	arg1	management					321:330	waste management	315:330	waste management	315:330	The chitinases find several applications in waste management and pest control.					
32713255	2	53	theme	N-acetyl-glucosamine	206:225	arg1	units					227:231	N-acetyl-glucosamine units	206:231	N-acetyl-glucosamine units linked through (1-4)-glycosidic bond	206:268	Chitinase is responsible for the breaking down of chitin to N-acetyl-glucosamine units linked through (1-4)-glycosidic bond.					
32713255	13	54	with	line	1419:1422	arg1	analysis					1442:1449	experimental analysis	1429:1449	experimental analysis	1429:1449	The validation study suggested that the highest activity of chitinase as predicted by ANN is in line with experimental analysis.					
32713255	5	55	theme	MTCC	585:588	arg1	9331					590:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	fungus Thermomyces lanuginosus MTCC 9331	554:593	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	6	56	theme	artificial	681:690	arg1	ANN					708:710	ANN	708:710	ANN	708:710	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	56	theme	artificial	681:690	arg1	network					699:705	artificial neural network	681:705	artificial neural network (ANN)	681:711	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	56	theme	artificial	681:690	arg1	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	14	57	theme	different	1476:1484	arg1	approaches					1498:1507	three different statistical approaches	1470:1507	three different statistical approaches	1470:1507	The comparison of three different statistical approaches suggested that ANN gives better optimization results compared to the GA and RSM study.					
32713255	3	58	theme	several	291:297	arg1	applications					299:310	several applications	291:310	several applications in waste management and pest control	291:347	The chitinases find several applications in waste management and pest control.					
32713255	12	59	theme	observed	1292:1299	arg1	data					1301:1304	the observed data	1288:1304	the observed data	1288:1304	The predicted chitinase activity was also closer to the observed data at these levels.					
32713255	10	60	theme	feed-forward	979:990	arg1	algorithm					992:1000	A multilayer feed-forward algorithm	966:1000	A multilayer feed-forward algorithm	966:1000	A multilayer feed-forward algorithm was performed for ANN, i.e., Levenberg-Marquardt, Bayesian Regularization, and Scaled Conjugate Gradient.					
32713255	4	61	theme	high	354:357	arg1	key					424:426	key	424:426	key	424:426	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	4	61	theme	high	354:357	arg1	yield					359:363	The high yield	350:363	The high yield with characteristics thermal stability of chitinase	350:415	The high yield with characteristics thermal stability of chitinase is the key to their industrial application.					
32713255	1	62	theme	genetic	127:133	arg1	algorithm					135:143	genetic algorithm	127:143	genetic algorithm	127:143	by using artificial neural network and genetic algorithm.					
32713255	2	63	theme	chitin	196:201	arg1	down					188:191	the breaking down	175:191	the breaking down of chitin to N-acetyl-glucosamine units linked through (1-4)-glycosidic bond	175:268	Chitinase is responsible for the breaking down of chitin to N-acetyl-glucosamine units linked through (1-4)-glycosidic bond.					
32713255	2	64	theme	-glycosidic	253:263	arg1	bond					265:268	(1-4)-glycosidic bond	248:268	(1-4)-glycosidic bond	248:268	Chitinase is responsible for the breaking down of chitin to N-acetyl-glucosamine units linked through (1-4)-glycosidic bond.					
32713255	5	65	theme	chitinase	527:535	arg1	production					537:546	chitinase production	527:546	chitinase production using fungus Thermomyces lanuginosus MTCC 9331	527:593	Therefore, the present work focuses on parameter optimization for chitinase production using fungus Thermomyces lanuginosus MTCC 9331.					
32713255	3	66	from	applications	299:310	arg1	management					321:330	waste management	315:330	waste management	315:330	The chitinases find several applications in waste management and pest control.					
32713255	3	66	from	applications	299:310	arg1	control					341:347	pest control	336:347	pest control	336:347	The chitinases find several applications in waste management and pest control.					
32713255	3	67	theme	pest	336:339	arg1	control					341:347	pest control	336:347	pest control	336:347	The chitinases find several applications in waste management and pest control.					
32713255	11	68	theme	predicted	1116:1124	arg1	values					1126:1131	predicted values	1116:1131	The ANN predicted values	1108:1131	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	6	69	used	used	745:748	arg2	algorithm					725:733	genetic algorithm	717:733	genetic algorithm (GA)	717:738	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	69	used	used	745:748	arg2	approaches					625:634	Three different optimization approaches	596:634	Three different optimization approaches	596:634	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	69	used	used	745:748	arg2	network					699:705	artificial neural network	681:705	artificial neural network (ANN)	681:711	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	6	69	used	used	745:748	arg2	methodology					662:672	response surface methodology	645:672	response surface methodology (RSM)	645:678	Three different optimization approaches, namely, response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) were used.					
32713255	13	70	dep	activity	1371:1378	arg1	predicted					1396:1404	predicted	1396:1404	predicted by ANN	1396:1411	The validation study suggested that the highest activity of chitinase as predicted by ANN is in line with experimental analysis.					
32713255	11	71	theme	higher	1138:1143	arg1	activity					1155:1162	higher chitinase activity	1138:1162	higher chitinase activity	1138:1162	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	11	72	dep	102.24 U/L	1171:1180	arg1	i.e.					1165:1168	i.e.	1165:1168	i.e.	1165:1168	The ANN predicted values gave higher chitinase activity, i.e., 102.24 U/L as compared to RSM-predicted values, i.e., 88.38 U/L.					
32713255	8	73	theme	composite	834:842	arg1	design					844:849	The central composite design	822:849	The central composite design with RSM	822:858	The central composite design with RSM was used for the optimization of the process parameters.					
32713255	10	74	dep	Levenberg-Marquardt	1031:1049	arg1	i.e.					1025:1028	i.e.	1025:1028	i.e.	1025:1028	A multilayer feed-forward algorithm was performed for ANN, i.e., Levenberg-Marquardt, Bayesian Regularization, and Scaled Conjugate Gradient.					
34552909	9	0	theme	Most	1515:1518	arg1	glycosites					1520:1529	Most glycosites	1515:1529	Most glycosites	1515:1529	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	4	1	with	de-N-glycosylation	567:584	arg1	trypsin/Glu-C					548:560	trypsin/Glu-C	548:560	trypsin/Glu-C	548:560	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	1	with	de-N-glycosylation	567:584	arg1	trypsin					536:542	trypsin	536:542	trypsin	536:542	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	1	2	theme	membrane	244:251	arg1	fusion					253:258	membrane fusion	244:258	membrane fusion	244:258	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	9	3	mod	modified	1536:1543	arg1	glycosites					1520:1529	Most glycosites	1515:1529	Most glycosites	1515:1529	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	3	mod	modified	1536:1543	arg3	O-glycans					1559:1567	sialylated O-glycans	1548:1567	sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2)	1548:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	3	mod	modified	1536:1543	arg3	NeuAc					1593:1597	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	3	mod	modified	1536:1543	arg3	NeuAc					1623:1627	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	11	4	theme	binding	1896:1902	arg1	mechanism					1904:1912	the viral binding mechanism	1886:1912	the viral binding mechanism	1886:1912	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	1	5	theme	respiratory	156:166	arg1	coronavirus					177:187	severe acute respiratory syndrome coronavirus 2	143:189	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	143:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	1	5	theme	respiratory	156:166	arg1	SARS-CoV-2					192:201	SARS-CoV-2	192:201	SARS-CoV-2	192:201	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	5	6	gly	O-glycosites	870:881	arg2	O-glycosites					870:881	43 O-glycosites	867:881	43 O-glycosites	867:881	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	10	7	theme	S	1673:1673	arg1	O-glycoprotein					1689:1702	an O-glycoprotein	1686:1702	an O-glycoprotein	1686:1702	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	10	7	theme	S	1673:1673	arg1	protein					1675:1681	the SARS-CoV-2 S protein	1658:1681	the SARS-CoV-2 S protein	1658:1681	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	6	8	mod	modified	1140:1147	arg1	glycosites					1124:1133	Most glycosites	1119:1133	Most glycosites	1119:1133	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	8	mod	modified	1140:1147	arg3	O-glycans					1167:1175	non-sialylated O-glycans	1152:1175	non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1)	1152:1214	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	8	mod	modified	1140:1147	arg3	HexNAc					1185:1190	HexNAc(1)	1185:1193	HexNAc(1)	1185:1193	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	8	mod	modified	1140:1147	arg3	HexNAc					1199:1204	HexNAc(1)	1199:1207	HexNAc(1)	1199:1207	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	1	9	theme	coronavirus	177:187	arg1	surface					132:138	the surface	128:138	the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	128:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	9	10	dep	NeuAc	1593:1597	arg1	1					1591:1591	1	1591:1591	1	1591:1591	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	4	11	theme	proteins	661:668	arg1	pattern					648:654	the GalNAc-type O-glycosylation pattern	616:654	the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them	616:741	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	11	12	theme	drugs	1982:1986	arg1	development					1945:1955	the development	1941:1955	the development of vaccines and targeted drugs	1941:1986	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	5	13	theme	insect	1086:1091	arg1	protein					1110:1116	the insect cell-expressed S protein	1082:1116	the insect cell-expressed S protein	1082:1116	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	14	theme	S	1108:1108	arg1	protein					1110:1116	the insect cell-expressed S protein	1082:1116	the insect cell-expressed S protein	1082:1116	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	8	15	theme	O-glycosylation	1471:1485	arg1	occupancy					1487:1495	O-glycosylation occupancy	1471:1495	O-glycosylation occupancy	1471:1495	However, the measurement of O-glycosylation occupancy hasn't been made.					
34552909	11	16	theme	O-glycosylation	1798:1812	arg1	landscapes					1814:1823	These comprehensive O-glycosylation landscapes	1778:1823	These comprehensive O-glycosylation landscapes of the S protein	1778:1840	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	9	17	dep	NeuAc	1623:1627	arg1	1					1621:1621	1	1621:1621	1	1621:1621	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	5	18	theme	collision-induced	916:932	arg1	dissociation					934:945	higher energy collision-induced dissociation	902:945	higher energy collision-induced dissociation (HCD)	902:951	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	18	theme	collision-induced	916:932	arg1	HCD					948:950	HCD	948:950	HCD	948:950	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	6	19	gly	non-sialylated	1152:1165	arg1	HexNAc					1199:1204	HexNAc(1)	1199:1207	HexNAc(1)	1199:1207	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	19	gly	non-sialylated	1152:1165	arg1	O-glycans					1167:1175	non-sialylated O-glycans	1152:1175	non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1)	1152:1214	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	19	gly	non-sialylated	1152:1165	arg1	HexNAc					1185:1190	HexNAc(1)	1185:1193	HexNAc(1)	1185:1193	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	4	20	theme	common	711:716	arg1	O-glycans					718:726	the six most common O-glycans	698:726	the six most common O-glycans occupying them	698:741	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	7	21	gly	O-glycopeptides	1291:1305	arg2	O-glycopeptides					1291:1305	407 intact O-glycopeptides	1280:1305	407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites	1280:1359	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	1	22	theme	severe	143:148	arg1	coronavirus					177:187	severe acute respiratory syndrome coronavirus 2	143:189	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	143:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	1	22	theme	severe	143:148	arg1	SARS-CoV-2					192:201	SARS-CoV-2	192:201	SARS-CoV-2	192:201	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	5	23	gly	O-glycosites	964:975	arg2	O-glycosites					964:975	three O-glycosites	958:975	three O-glycosites	958:975	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	1	24	theme	glycosylated	69:80	arg1	proteins					92:99	The densely glycosylated spike (S) proteins	57:99	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	57:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	3	25	theme	SARS-CoV-2	420:429	arg1	proteins					433:440	SARS-CoV-2 S proteins	420:440	SARS-CoV-2 S proteins	420:440	Herein, we report the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry.					
34552909	5	26	theme	transfer/higher	1017:1031	arg1	dissociation					1058:1069	electron transfer/higher energy collision-induced dissociation	1008:1069	electron transfer/higher energy collision-induced dissociation (EThcD)	1008:1077	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	26	theme	transfer/higher	1017:1031	arg1	EThcD					1072:1076	EThcD	1072:1076	EThcD	1072:1076	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	9	27	gly	glycosites	1520:1529	arg2	glycosites					1520:1529	Most glycosites	1515:1529	Most glycosites	1515:1529	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	4	28	theme	Byonic	748:753	arg1	identification					755:768	Byonic identification	748:768	Byonic identification	748:768	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	0	29	theme	O-Glycosylation	0:14	arg1	Landscapes					16:25	O-Glycosylation Landscapes	0:25	O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins	0:54	O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins.					
34552909	7	30	theme	34	1319:1320	arg1	sequences					1331:1339	34 peptides sequences	1319:1339	34 peptides sequences	1319:1339	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	11	31	theme	novel	1866:1870	arg1	insights					1872:1879	novel insights	1866:1879	novel insights into the viral binding mechanism	1866:1912	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	11	32	theme	S	1832:1832	arg1	protein					1834:1840	the S protein	1828:1840	the S protein	1828:1840	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	5	33	theme	peptides	844:851	arg1	sequences					853:861	50 peptides sequences	841:861	50 peptides sequences	841:861	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	9	34	gly	sialylated	1548:1557	arg1	O-glycans					1559:1567	sialylated O-glycans	1548:1567	sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2)	1548:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	34	gly	sialylated	1548:1557	arg1	NeuAc					1593:1597	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	34	gly	sialylated	1548:1557	arg1	NeuAc					1623:1627	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	3	35	theme	proteins	433:440	arg1	landscapes					406:415	the O-glycosylation landscapes	386:415	the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry	386:508	Herein, we report the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry.					
34552909	0	36	theme	Spike	41:45	arg1	Proteins					47:54	SARS-CoV-2 Spike Proteins	30:54	SARS-CoV-2 Spike Proteins	30:54	O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins.					
34552909	7	37	theme	intact	1284:1289	arg1	O-glycopeptides					1291:1305	407 intact O-glycopeptides	1280:1305	407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites	1280:1359	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	6	38	gly	glycosites	1124:1133	arg2	glycosites					1124:1133	Most glycosites	1119:1133	Most glycosites	1119:1133	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	4	39	with	digestion	521:529	arg1	trypsin/Glu-C					548:560	trypsin/Glu-C	548:560	trypsin/Glu-C	548:560	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	39	with	digestion	521:529	arg1	trypsin					536:542	trypsin	536:542	trypsin	536:542	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	2	40	gly	22 N-glycosites	297:311	arg2	22 N-glycosites					297:311	22 N-glycosites	297:311	22 N-glycosites	297:311	We have previously reported all the 22 N-glycosites and site-specific N-glycans in the S protein protomer.					
34552909	5	41	theme	collision-induced	1040:1056	arg1	dissociation					1058:1069	electron transfer/higher energy collision-induced dissociation	1008:1069	electron transfer/higher energy collision-induced dissociation (EThcD)	1008:1077	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	41	theme	collision-induced	1040:1056	arg1	EThcD					1072:1076	EThcD	1072:1076	EThcD	1072:1076	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	7	42	theme	S1	1268:1269	arg1	subunit					1271:1277	the human cell-expressed S protein S1 subunit	1233:1277	the human cell-expressed S protein S1 subunit	1233:1277	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	6	43	theme	non-sialylated	1152:1165	arg1	HexNAc					1199:1204	HexNAc(1)	1199:1207	HexNAc(1)	1199:1207	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	43	theme	non-sialylated	1152:1165	arg1	O-glycans					1167:1175	non-sialylated O-glycans	1152:1175	non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1)	1152:1214	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	6	43	theme	non-sialylated	1152:1165	arg1	HexNAc					1185:1190	HexNAc(1)	1185:1193	HexNAc(1)	1185:1193	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	5	44	gly	O-glycopeptides	813:827	arg2	O-glycopeptides					813:827	255 intact O-glycopeptides	802:827	255 intact O-glycopeptides composed of 50 peptides sequences	802:861	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	2	45	theme	protein	350:356	arg1	protomer					358:365	the S protein protomer	344:365	the S protein protomer	344:365	We have previously reported all the 22 N-glycosites and site-specific N-glycans in the S protein protomer.					
34552909	7	46	gly	O-glycosites	1392:1403	arg2	O-glycosites					1392:1403	11 O-glycosites	1389:1403	11 O-glycosites	1389:1403	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	7	47	theme	S	1258:1258	arg1	subunit					1271:1277	the human cell-expressed S protein S1 subunit	1233:1277	the human cell-expressed S protein S1 subunit	1233:1277	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	4	48	theme	GalNAc-type	620:630	arg1	pattern					648:654	the GalNAc-type O-glycosylation pattern	616:654	the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them	616:741	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	10	49	gly	O-glycoprotein	1689:1702	arg1	O-glycoprotein					1689:1702	an O-glycoprotein	1686:1702	an O-glycoprotein	1686:1702	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	10	49	gly	O-glycoprotein	1689:1702	arg1	protein					1675:1681	the SARS-CoV-2 S protein	1658:1681	the SARS-CoV-2 S protein	1658:1681	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	7	50	theme	human	1237:1241	arg1	subunit					1271:1277	the human cell-expressed S protein S1 subunit	1233:1277	the human cell-expressed S protein S1 subunit	1233:1277	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	4	51	theme	PNGase	592:597	arg1	F					599:599	PNGase F	592:599	PNGase F	592:599	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	52	gly	O-glycosites	681:692	arg2	O-glycosites					681:692	O-glycosites	681:692	O-glycosites	681:692	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	9	53	theme	HexNAc	1607:1612	arg1	NeuAc					1623:1627	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	3	54	theme	high-resolution	476:490	arg1	spectrometry					497:508	high-resolution mass spectrometry	476:508	high-resolution mass spectrometry	476:508	Herein, we report the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry.					
34552909	11	55	theme	viral	1890:1894	arg1	mechanism					1904:1912	the viral binding mechanism	1886:1912	the viral binding mechanism	1886:1912	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	1	56	theme	acute	150:154	arg1	coronavirus					177:187	severe acute respiratory syndrome coronavirus 2	143:189	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	143:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	1	56	theme	acute	150:154	arg1	SARS-CoV-2					192:201	SARS-CoV-2	192:201	SARS-CoV-2	192:201	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	1	57	theme	syndrome	168:175	arg1	coronavirus					177:187	severe acute respiratory syndrome coronavirus 2	143:189	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	143:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	1	57	theme	syndrome	168:175	arg1	SARS-CoV-2					192:201	SARS-CoV-2	192:201	SARS-CoV-2	192:201	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	7	58	gly	O-glycosites	1348:1359	arg2	O-glycosites					1348:1359	30 O-glycosites	1345:1359	30 O-glycosites	1345:1359	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	9	59	theme	Hex	1616:1618	arg1	NeuAc					1623:1627	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	10	60	theme	SARS-CoV-2	1662:1671	arg1	O-glycoprotein					1689:1702	an O-glycoprotein	1686:1702	an O-glycoprotein	1686:1702	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	10	60	theme	SARS-CoV-2	1662:1671	arg1	protein					1675:1681	the SARS-CoV-2 S protein	1658:1681	the SARS-CoV-2 S protein	1658:1681	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	11	61	theme	vaccines	1960:1967	arg1	development					1945:1955	the development	1941:1955	the development of vaccines and targeted drugs	1941:1986	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	2	62	theme	site-specific	317:329	arg1	N-glycans					331:339	all the 22 N-glycosites and site-specific N-glycans	289:339	all the 22 N-glycosites and site-specific N-glycans	289:339	We have previously reported all the 22 N-glycosites and site-specific N-glycans in the S protein protomer.					
34552909	5	63	theme	higher	902:907	arg1	dissociation					934:945	higher energy collision-induced dissociation	902:945	higher energy collision-induced dissociation (HCD)	902:951	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	63	theme	higher	902:907	arg1	HCD					948:950	HCD	948:950	HCD	948:950	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	4	64	theme	S	659:659	arg1	proteins					661:668	S proteins	659:668	S proteins	659:668	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	64	theme	S	659:659	arg1	O-glycosites					681:692	O-glycosites	681:692	O-glycosites	681:692	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	64	theme	S	659:659	arg1	O-glycans					718:726	the six most common O-glycans	698:726	the six most common O-glycans occupying them	698:741	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	5	65	theme	cell-expressed	1093:1106	arg1	protein					1110:1116	the insect cell-expressed S protein	1082:1116	the insect cell-expressed S protein	1082:1116	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	10	66	theme	O-glycan	1726:1733	arg1	compositions					1735:1746	the O-glycosites and O-glycan compositions	1705:1746	the O-glycosites and O-glycan compositions	1705:1746	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	11	67	theme	targeted	1973:1980	arg1	drugs					1982:1986	targeted drugs	1973:1986	targeted drugs	1973:1986	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	8	68	theme	occupancy	1487:1495	arg1	measurement					1456:1466	the measurement	1452:1466	the measurement of O-glycosylation occupancy	1452:1495	However, the measurement of O-glycosylation occupancy hasn't been made.					
34552909	5	69	theme	electron	1008:1015	arg1	dissociation					1058:1069	electron transfer/higher energy collision-induced dissociation	1008:1069	electron transfer/higher energy collision-induced dissociation (EThcD)	1008:1077	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	69	theme	electron	1008:1015	arg1	EThcD					1072:1076	EThcD	1072:1076	EThcD	1072:1076	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	1	70	gly	glycosylated	69:80	arg1	proteins					92:99	The densely glycosylated spike (S) proteins	57:99	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	57:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	10	71	theme	O-glycosites	1709:1720	arg1	compositions					1735:1746	the O-glycosites and O-glycan compositions	1705:1746	the O-glycosites and O-glycan compositions	1705:1746	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	5	72	theme	energy	909:914	arg1	dissociation					934:945	higher energy collision-induced dissociation	902:945	higher energy collision-induced dissociation (HCD)	902:951	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	72	theme	energy	909:914	arg1	HCD					948:950	HCD	948:950	HCD	948:950	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	4	73	theme	manual	774:779	arg1	validation					781:790	manual validation	774:790	manual validation	774:790	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	74	gly	O-glycosylation	632:646	arg1	proteins					661:668	S proteins	659:668	S proteins	659:668	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	74	gly	O-glycosylation	632:646	arg1	O-glycosites					681:692	O-glycosites	681:692	O-glycosites	681:692	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	4	74	gly	O-glycosylation	632:646	arg1	O-glycans					718:726	the six most common O-glycans	698:726	the six most common O-glycans occupying them	698:741	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	9	75	theme	sialylated	1548:1557	arg1	O-glycans					1559:1567	sialylated O-glycans	1548:1567	sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2)	1548:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	75	theme	sialylated	1548:1557	arg1	NeuAc					1593:1597	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	9	75	theme	sialylated	1548:1557	arg1	NeuAc					1623:1627	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	HexNAc(1)Hex (1)NeuAc (2)	1607:1631	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	7	76	theme	peptides	1322:1329	arg1	sequences					1331:1339	34 peptides sequences	1319:1339	34 peptides sequences	1319:1339	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	3	77	theme	S	431:431	arg1	proteins					433:440	SARS-CoV-2 S proteins	420:440	SARS-CoV-2 S proteins	420:440	Herein, we report the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry.					
34552909	1	78	theme	S	89:89	arg1	proteins					92:99	The densely glycosylated spike (S) proteins	57:99	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	57:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	6	79	theme	Most	1119:1122	arg1	glycosites					1124:1133	Most glycosites	1119:1133	Most glycosites	1119:1133	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	10	80	theme	host	1762:1765	arg1	type					1772:1775	the host cell type	1758:1775	the host cell type	1758:1775	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	11	81	theme	comprehensive	1784:1796	arg1	landscapes					1814:1823	These comprehensive O-glycosylation landscapes	1778:1823	These comprehensive O-glycosylation landscapes of the S protein	1778:1840	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	0	82	theme	SARS-CoV-2	30:39	arg1	Proteins					47:54	SARS-CoV-2 Spike Proteins	30:54	SARS-CoV-2 Spike Proteins	30:54	O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins.					
34552909	9	83	theme	HexNAc	1577:1582	arg1	NeuAc					1593:1597	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	1	84	theme	spike	82:86	arg1	proteins					92:99	The densely glycosylated spike (S) proteins	57:99	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	57:202	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	11	85	theme	protein	1834:1840	arg1	landscapes					1814:1823	These comprehensive O-glycosylation landscapes	1778:1823	These comprehensive O-glycosylation landscapes of the S protein	1778:1840	These comprehensive O-glycosylation landscapes of the S protein are expected to provide novel insights into the viral binding mechanism and present a strategy for the development of vaccines and targeted drugs.					
34552909	0	86	theme	Proteins	47:54	arg1	Landscapes					16:25	O-Glycosylation Landscapes	0:25	O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins	0:54	O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins.					
34552909	3	87	theme	O-glycosylation	390:404	arg1	landscapes					406:415	the O-glycosylation landscapes	386:415	the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry	386:508	Herein, we report the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry.					
34552909	5	88	theme	intact	806:811	arg1	O-glycopeptides					813:827	255 intact O-glycopeptides	802:827	255 intact O-glycopeptides composed of 50 peptides sequences	802:861	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	9	89	theme	Hex	1586:1588	arg1	NeuAc					1593:1597	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	HexNAc(1)Hex (1)NeuAc (1)	1577:1601	Most glycosites were modified by sialylated O-glycans such as HexNAc(1)Hex (1)NeuAc (1) and HexNAc(1)Hex (1)NeuAc (2).					
34552909	3	90	theme	mass	492:495	arg1	spectrometry					497:508	high-resolution mass spectrometry	476:508	high-resolution mass spectrometry	476:508	Herein, we report the O-glycosylation landscapes of SARS-CoV-2 S proteins, which were characterized through high-resolution mass spectrometry.					
34552909	5	91	theme	energy	1033:1038	arg1	dissociation					1058:1069	electron transfer/higher energy collision-induced dissociation	1008:1069	electron transfer/higher energy collision-induced dissociation (EThcD)	1008:1077	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	5	91	theme	energy	1033:1038	arg1	EThcD					1072:1076	EThcD	1072:1076	EThcD	1072:1076	Finally, 255 intact O-glycopeptides composed of 50 peptides sequences and 43 O-glycosites were discovered by higher energy collision-induced dissociation (HCD), and three O-glycosites were confidently identified by electron transfer/higher energy collision-induced dissociation (EThcD) in the insect cell-expressed S protein.					
34552909	7	92	theme	protein	1260:1266	arg1	subunit					1271:1277	the human cell-expressed S protein S1 subunit	1233:1277	the human cell-expressed S protein S1 subunit	1233:1277	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	10	93	theme	cell	1767:1770	arg1	type					1772:1775	the host cell type	1758:1775	the host cell type	1758:1775	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
34552909	2	94	theme	22 N-glycosites	297:311	arg1	N-glycans					331:339	all the 22 N-glycosites and site-specific N-glycans	289:339	all the 22 N-glycosites and site-specific N-glycans	289:339	We have previously reported all the 22 N-glycosites and site-specific N-glycans in the S protein protomer.					
34552909	1	95	theme	viral	215:219	arg1	attachment					221:230	viral attachment	215:230	viral attachment	215:230	The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion.					
34552909	7	96	theme	cell-expressed	1243:1256	arg1	subunit					1271:1277	the human cell-expressed S protein S1 subunit	1233:1277	the human cell-expressed S protein S1 subunit	1233:1277	In contrast, in the human cell-expressed S protein S1 subunit, 407 intact O-glycopeptides composed of 34 peptides sequences and 30 O-glycosites were discovered by HCD, and 11 O-glycosites were unambiguously assigned by EThcD.					
34552909	4	97	theme	O-glycosylation	632:646	arg1	pattern					648:654	the GalNAc-type O-glycosylation pattern	616:654	the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them	616:741	Following digestion with trypsin and trypsin/Glu-C, and de-N-glycosylation using PNGase F, we determined the GalNAc-type O-glycosylation pattern of S proteins, including O-glycosites and the six most common O-glycans occupying them, via Byonic identification and manual validation.					
34552909	6	98	dep	HexNAc	1185:1190	arg1	Hex					1208:1210	Hex (1)	1208:1214	Hex (1)	1208:1214	Most glycosites were modified by non-sialylated O-glycans such as HexNAc(1) and HexNAc(1)Hex (1).					
34552909	2	99	theme	S	348:348	arg1	protomer					358:365	the S protein protomer	344:365	the S protein protomer	344:365	We have previously reported all the 22 N-glycosites and site-specific N-glycans in the S protein protomer.					
34552909	10	100	gly	O-glycosites	1709:1720	arg2	O-glycosites					1709:1720	O-glycosites	1709:1720	O-glycosites	1709:1720	Our results reveal that the SARS-CoV-2 S protein is an O-glycoprotein; the O-glycosites and O-glycan compositions vary with the host cell type.					
32817557	4	0	theme	glycan-based	655:666	arg1	motifs					668:673	distinct peptide- and glycan-based motifs	633:673	distinct peptide- and glycan-based motifs	633:673	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	1	1	theme	physiological	231:243	arg1	roles					245:249	key physiological roles	227:249	key physiological roles	227:249	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	5	2	theme	catalytic	760:768	arg1	residues					770:777	catalytic residues	760:777	catalytic residues of two such enzymes	760:797	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	5	2	theme	catalytic	760:768	arg1	enzymes					791:797	two such enzymes	782:797	two such enzymes	782:797	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	7	3	theme	premalignant	1160:1171	arg1	tissues					1173:1179	malignant and premalignant tissues	1146:1179	malignant and premalignant tissues	1146:1179	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	2	4	from	common	319:324	arg1	cancer					366:371	cancer	366:371	cancer	366:371	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	4	from	common	319:324	arg1	variety					331:337	a variety	329:337	a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases	329:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	4	from	common	319:324	arg1	addition					255:262	addition	255:262	addition	255:262	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	4	from	common	319:324	arg1	diseases					414:421	inflammatory bowel diseases	395:421	inflammatory bowel diseases	395:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	4	from	common	319:324	arg1	diseases					348:355	human diseases	342:355	human diseases	342:355	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	4	from	common	319:324	arg1	fibrosis					381:388	cystic fibrosis	374:388	cystic fibrosis	374:388	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	1	5	theme	broad	160:164	arg1	range					166:170	a broad range	158:170	a broad range	158:170	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	6	6	theme	glycoforms	997:1006	arg1	range					988:992	a wide range	981:992	a wide range of glycoforms	981:1006	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	0	7	theme	glycoproteins	93:105	arg1	visualization					63:75	visualization	63:75	visualization of mucin-domain glycoproteins	63:105	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	0	7	theme	glycoproteins	93:105	arg1	proteolysis					35:45	selective proteolysis	25:45	selective proteolysis	25:45	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	0	7	theme	glycoproteins	93:105	arg1	detection					48:56	detection	48:56	detection	48:56	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	9	8	from	differences	1533:1543	arg1	cleavage					1554:1561	mucin cleavage and expression patterns	1548:1585	cleavage	1554:1561	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	9	8	from	differences	1533:1543	arg1	patterns					1578:1585	mucin cleavage and expression patterns	1548:1585	patterns	1578:1585	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	4	9	theme	mucin-selective	719:733	arg1	proteolysis					735:745	mucin-selective proteolysis	719:745	mucin-selective proteolysis	719:745	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	0	10	theme	mucin-domain	80:91	arg1	glycoproteins					93:105	mucin-domain glycoproteins	80:105	mucin-domain glycoproteins	80:105	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	6	11	theme	pan-mucin	897:905	arg1	StcEE447D					882:890	StcEE447D	882:890	StcEE447D	882:890	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	6	11	theme	pan-mucin	897:905	arg1	tolerant					969:976	tolerant	969:976	tolerant	969:976	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	6	11	theme	pan-mucin	897:905	arg1	stain					907:911	a pan-mucin stain	895:911	a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms	895:1006	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	7	12	theme	malignant	1146:1154	arg1	tissues					1173:1179	malignant and premalignant tissues	1146:1179	malignant and premalignant tissues	1146:1179	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	3	13	theme	mucin	528:532	arg1	domains					534:540	mucin domains	528:540	mucin domains	528:540	These correlations have been challenging to uncover and establish because tools that specifically probe mucin domains are lacking.					
32817557	4	14	theme	mucin	615:619	arg1	domains					621:627	mucin domains	615:627	mucin domains	615:627	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	7	15	attach	derived	1021:1027	arg1	thetaiotaomicron					1046:1061	Bacteroides thetaiotaomicron	1034:1061	Bacteroides thetaiotaomicron	1034:1061	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	7	15	attach	derived	1021:1027	arg2	BT4244E575A					1009:1019	BT4244E575A	1009:1019	BT4244E575A derived from Bacteroides thetaiotaomicron	1009:1061	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	1	16	located	found	149:153	arg1	range					166:170	a broad range	158:170	a broad range	158:170	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	1	16	located	found	149:153	arg2	domains					137:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	5	17	theme	binding	830:836	arg1	agents					838:843	mucin-selective binding agents	814:843	mucin-selective binding agents	814:843	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	4	18	theme	proteases	593:601	arg1	panel					574:578	a panel	572:578	a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs	572:673	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	9	19	theme	patients	1515:1522	arg1	characterization					1495:1510	characterization	1495:1510	characterization of patients based on differences in mucin cleavage and expression patterns	1495:1585	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	4	20	theme	peptide-	642:649	arg1	motifs					668:673	distinct peptide- and glycan-based motifs	633:673	distinct peptide- and glycan-based motifs	633:673	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	5	21	theme	retained	850:857	arg1	preferences					869:879	retained glycoform preferences	850:879	retained glycoform preferences	850:879	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	9	22	theme	mucin	1548:1552	arg1	cleavage					1554:1561	mucin cleavage and expression patterns	1548:1585	cleavage	1554:1561	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	5	23	theme	enzymes	791:797	arg1	residues					770:777	catalytic residues	760:777	catalytic residues of two such enzymes	760:797	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	5	23	theme	enzymes	791:797	arg1	enzymes					791:797	two such enzymes	782:797	two such enzymes	782:797	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	7	24	theme	core	1103:1106	arg1	structures					1110:1119	truncated, asialylated core 1 structures	1080:1119	truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues	1080:1179	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	8	25	theme	point	1232:1236	arg1	mutants					1238:1244	these catalytically inactive point mutants	1203:1244	these catalytically inactive point mutants	1203:1244	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	5	26	theme	glycoform	859:867	arg1	preferences					869:879	retained glycoform preferences	850:879	retained glycoform preferences	850:879	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	0	27	theme	enzymatic	3:11	arg1	toolkit					13:19	An enzymatic toolkit	0:19	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins	0:105	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	9	28	theme	enzymatic	1396:1404	arg1	toolkit					1406:1412	our enzymatic toolkit	1392:1412	our enzymatic toolkit	1392:1412	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	2	29	from	variety	331:337	arg1	common					319:324	common	319:324	common	319:324	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	1	30	theme	cell	175:178	arg1	surface					180:186	cell surface	175:186	cell surface	175:186	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	2	31	theme	bowel	408:412	arg1	diseases					414:421	inflammatory bowel diseases	395:421	inflammatory bowel diseases	395:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	6	32	theme	wide	983:986	arg1	range					988:992	a wide range	981:992	a wide range of glycoforms	981:1006	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	8	33	theme	glycoproteins	1304:1316	arg1	visualization					1274:1286	visualization	1274:1286	visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry	1274:1374	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	8	33	theme	glycoproteins	1304:1316	arg1	detection					1260:1268	robust detection	1253:1268	robust detection	1253:1268	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	0	34	gly	glycoproteins	93:105	arg1	glycoproteins					93:105	mucin-domain glycoproteins	80:105	mucin-domain glycoproteins	80:105	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	1	35	theme	surface	180:186	arg1	range					166:170	a broad range	158:170	a broad range	158:170	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	2	36	theme	inflammatory	395:406	arg1	diseases					414:421	inflammatory bowel diseases	395:421	inflammatory bowel diseases	395:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	5	37	theme	mucin-selective	814:828	arg1	agents					838:843	mucin-selective binding agents	814:843	mucin-selective binding agents	814:843	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	9	38	theme	ascites	1417:1423	arg1	fluid					1425:1429	ascites fluid and tissue slices	1417:1447	fluid	1425:1429	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	1	39	gly	O-glycosylated	116:129	arg1	domains					137:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	2	40	theme	mucin	280:284	arg1	expression					286:295	mucin expression	280:295	mucin expression	280:295	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	1	41	theme	secreted	192:199	arg1	proteins					201:208	secreted proteins	192:208	secreted proteins	192:208	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	7	42	theme	truncated	1080:1088	arg1	structures					1110:1119	truncated, asialylated core 1 structures	1080:1119	truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues	1080:1179	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	1	43	theme	proteins	201:208	arg1	range					166:170	a broad range	158:170	a broad range	158:170	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	4	44	theme	distinct	633:640	arg1	motifs					668:673	distinct peptide- and glycan-based motifs	633:673	distinct peptide- and glycan-based motifs	633:673	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	2	45	theme	cystic	374:379	arg1	fibrosis					381:388	cystic fibrosis	374:388	cystic fibrosis	374:388	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	0	46	theme	selective	25:33	arg1	proteolysis					35:45	selective proteolysis	25:45	selective proteolysis	25:45	An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins.					
32817557	9	47	theme	toolkit	1406:1412	arg1	Application					1377:1387	Application	1377:1387	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer	1377:1481	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	9	48	theme	expression	1567:1576	arg1	patterns					1578:1585	mucin cleavage and expression patterns	1548:1585	patterns	1578:1585	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	8	49	theme	robust	1253:1258	arg1	detection					1260:1268	robust detection	1253:1268	robust detection	1253:1268	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	6	50	attach	derived	913:919	arg2	StcEE447D					882:890	StcEE447D	882:890	StcEE447D	882:890	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	6	50	attach	derived	913:919	arg2	tolerant					969:976	tolerant	969:976	tolerant	969:976	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	6	50	attach	derived	913:919	arg2	stain					907:911	a pan-mucin stain	895:911	a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms	895:1006	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	6	50	attach	derived	913:919	arg1	coli					956:959	enterohemorrhagic Escherichia coli	926:959	enterohemorrhagic Escherichia coli	926:959	StcEE447D is a pan-mucin stain derived from enterohemorrhagic Escherichia coli that is tolerant to a wide range of glycoforms.					
32817557	9	51	theme	tissue	1435:1440	arg1	slices					1442:1447	ascites fluid and tissue slices	1417:1447	slices	1442:1447	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	7	52	theme	Bacteroides	1034:1044	arg1	thetaiotaomicron					1046:1061	Bacteroides thetaiotaomicron	1034:1061	Bacteroides thetaiotaomicron	1034:1061	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	1	53	theme	O-glycosylated	116:129	arg1	domains					137:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	5	54	theme	such	786:789	arg1	enzymes					791:797	two such enzymes	782:797	two such enzymes	782:797	By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences.					
32817557	1	55	theme	mucin	131:135	arg1	domains					137:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains	108:143	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
32817557	8	56	gly	glycoproteins	1304:1316	arg1	glycoproteins					1304:1316	mucin-domain glycoproteins	1291:1316	mucin-domain glycoproteins	1291:1316	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	9	57	from	patients	1454:1461	arg1	fluid					1425:1429	ascites fluid and tissue slices	1417:1447	fluid	1425:1429	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	9	57	from	patients	1454:1461	arg1	slices					1442:1447	ascites fluid and tissue slices	1417:1447	slices	1442:1447	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	8	58	theme	flow	1321:1324	arg1	cytometry					1326:1334	flow cytometry	1321:1334	flow cytometry	1321:1334	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	4	59	theme	enzymatic	697:705	arg1	toolkit					707:713	a diverse enzymatic toolkit	687:713	a diverse enzymatic toolkit for mucin-selective proteolysis	687:745	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	2	60	from	addition	255:262	arg1	common					319:324	common	319:324	common	319:324	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	9	61	theme	ovarian	1468:1474	arg1	cancer					1476:1481	ovarian cancer	1468:1481	ovarian cancer	1468:1481	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	4	62	theme	diverse	689:695	arg1	toolkit					707:713	a diverse enzymatic toolkit	687:713	a diverse enzymatic toolkit for mucin-selective proteolysis	687:745	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	7	63	dep	truncated	1080:1088	arg1	asialylated					1091:1101	asialylated	1091:1101	asialylated	1091:1101	BT4244E575A derived from Bacteroides thetaiotaomicron is selective for truncated, asialylated core 1 structures commonly associated with malignant and premalignant tissues.					
32817557	8	64	theme	Western	1337:1343	arg1	blot					1345:1348	Western blot	1337:1348	Western blot	1337:1348	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	8	65	theme	inactive	1223:1230	arg1	mutants					1238:1244	these catalytically inactive point mutants	1203:1244	these catalytically inactive point mutants	1203:1244	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	2	66	theme	diseases	348:355	arg1	diseases					414:421	inflammatory bowel diseases	395:421	inflammatory bowel diseases	395:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	66	theme	diseases	348:355	arg1	cancer					366:371	cancer	366:371	cancer	366:371	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	66	theme	diseases	348:355	arg1	fibrosis					381:388	cystic fibrosis	374:388	cystic fibrosis	374:388	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	66	theme	diseases	348:355	arg1	variety					331:337	a variety	329:337	a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases	329:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	66	theme	diseases	348:355	arg1	diseases					348:355	human diseases	342:355	human diseases	342:355	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	67	from	alterations	265:275	arg1	expression					286:295	mucin expression	280:295	mucin expression	280:295	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	67	from	alterations	265:275	arg1	glycosylation					301:313	glycosylation	301:313	glycosylation	301:313	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	4	68	theme	bacterial	583:591	arg1	proteases					593:601	bacterial proteases	583:601	bacterial proteases	583:601	Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis.					
32817557	8	69	theme	mucin-domain	1291:1302	arg1	glycoproteins					1304:1316	mucin-domain glycoproteins	1291:1316	mucin-domain glycoproteins	1291:1316	We demonstrated that these catalytically inactive point mutants enable robust detection and visualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.					
32817557	9	70	with	patients	1454:1461	arg1	cancer					1476:1481	ovarian cancer	1468:1481	ovarian cancer	1468:1481	Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns.					
32817557	2	71	theme	human	342:346	arg1	diseases					414:421	inflammatory bowel diseases	395:421	inflammatory bowel diseases	395:421	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	71	theme	human	342:346	arg1	cancer					366:371	cancer	366:371	cancer	366:371	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	71	theme	human	342:346	arg1	fibrosis					381:388	cystic fibrosis	374:388	cystic fibrosis	374:388	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	2	71	theme	human	342:346	arg1	diseases					348:355	human diseases	342:355	human diseases	342:355	In addition, alterations in mucin expression and glycosylation are common in a variety of human diseases, such as cancer, cystic fibrosis, and inflammatory bowel diseases.					
32817557	1	72	theme	key	227:229	arg1	roles					245:249	key physiological roles	227:249	key physiological roles	227:249	Densely O-glycosylated mucin domains are found in a broad range of cell surface and secreted proteins, where they play key physiological roles.					
34164984	0	0	theme	Cellulose/MoS2-Chitosan	92:114	arg1	Materials					130:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	4	1	theme	excellent	946:954	arg1	efficacy					970:977	excellent antibacterial efficacy	946:977	excellent antibacterial efficacy	946:977	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	6	2	theme	composite	1608:1616	arg1	nanomaterials					1618:1630	such composite nanomaterials	1603:1630	such composite nanomaterials	1603:1630	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	5	3	theme	pathogen	1459:1466	arg1	inactivation					1468:1479	synergistic pathogen inactivation	1447:1479	synergistic pathogen inactivation upon visible-light illumination	1447:1511	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	3	4	theme	bactericidal	884:895	arg1	activity					897:904	more efficient bactericidal activity	869:904	more efficient bactericidal activity	869:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	0	5	theme	Bacterial	82:90	arg1	Materials					130:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	3	6	theme	cellulose/MoS2-chitosan	686:708	arg1	materials					724:732	grown bacterial cellulose/MoS2-chitosan nanocomposite materials	670:732	in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity	662:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	6	theme	cellulose/MoS2-chitosan	686:708	arg1	BC/MoS2-CS					742:751	termed BC/MoS2-CS	735:751	termed BC/MoS2-CS	735:751	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	1	7	from	rise	186:189	arg1	prevalence					194:203	prevalence	194:203	prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics	194:277	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	4	8	theme	antibacterial	956:968	arg1	efficacy					970:977	excellent antibacterial efficacy	946:977	excellent antibacterial efficacy	946:977	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	6	9	theme	cell	1527:1530	arg1	cytotoxicity					1532:1543	No mammalian cell cytotoxicity	1514:1543	No mammalian cell cytotoxicity	1514:1543	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	5	10	theme	Mechanistic	1227:1237	arg1	studies					1239:1245	Mechanistic studies	1227:1245	Mechanistic studies	1227:1245	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	0	11	theme	Materials	130:138	arg1	Activity					56:63	Synergistic Photodynamic and Photothermal Antibacterial Activity	0:63	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.	0:171	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	1	12	theme	ever-increasing	386:400	arg1	diseases					291:298	infectious diseases	280:298	infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts	280:377	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	1	12	theme	ever-increasing	386:400	arg1	threat					402:407	an ever-increasing threat	383:407	an ever-increasing threat to public health	383:424	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	5	13	theme	chitosan	1281:1288	arg1	use					1265:1267	the use	1261:1267	the use of cationic chitosan	1261:1288	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	6	14	theme	BC/MoS2-CS	1566:1575	arg1	membrane					1577:1584	the BC/MoS2-CS membrane	1562:1584	the BC/MoS2-CS membrane	1562:1584	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	6	15	theme	mammalian	1517:1525	arg1	cytotoxicity					1532:1543	No mammalian cell cytotoxicity	1514:1543	No mammalian cell cytotoxicity	1514:1543	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	4	16	theme	log	1031:1033	arg1	%					1024:1024	99.988%	1018:1024	99.988% (3.9 log units)	1018:1040	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	16	theme	log	1031:1033	arg1	units					1035:1039	3.9 log units	1027:1039	3.9 log units	1027:1039	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	0	17	theme	Nanocomposite	116:128	arg1	Materials					130:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	1	18	from	surfaces	357:364	arg1	transmission					314:325	the transmission	310:325	the transmission of microbes from contaminated surfaces to new hosts	310:377	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	3	19	theme	efficient	874:882	arg1	activity					897:904	more efficient bactericidal activity	869:904	more efficient bactericidal activity	869:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	0	20	theme	Visible	145:151	arg1	Illumination					159:170	Visible Light Illumination	145:170	Visible Light Illumination	145:170	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	5	21	theme	cationic	1272:1279	arg1	chitosan					1281:1288	cationic chitosan	1272:1288	cationic chitosan	1272:1288	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	2	22	theme	novel	433:437	arg1	materials					439:447	novel materials	433:447	novel materials that can stem this crisis	433:473	Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need.					
34164984	1	23	theme	public	412:417	arg1	health					419:424	public health	412:424	public health	412:424	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	3	24	dep	in	662:663	arg1	situ					665:668	situ	665:668	situ	665:668	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	4	25	dep	illumination	1169:1180	arg1	nm					1210:1211	λ ≥ 420 nm	1202:1211	λ ≥ 420 nm	1202:1211	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	25	dep	illumination	1169:1180	arg1	lamp					1189:1192	xenon lamp	1183:1192	xenon lamp	1183:1192	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	25	dep	illumination	1169:1180	arg1	min					1221:1223	30 min	1218:1223	30 min	1218:1223	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	25	dep	illumination	1169:1180	arg1	W					1199:1199	500 W	1195:1199	500 W	1195:1199	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	26	theme	λ	1202:1202	arg1	nm					1210:1211	λ ≥ 420 nm	1202:1211	λ ≥ 420 nm	1202:1211	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	6	27	theme	functional	1650:1659	arg1	materials					1661:1669	functional materials	1650:1669	functional materials for infection control applications	1650:1704	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	0	28	theme	Photodynamic	12:23	arg1	Activity					56:63	Synergistic Photodynamic and Photothermal Antibacterial Activity	0:63	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.	0:171	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	2	29	theme	antimicrobial	512:524	arg1	mechanisms					526:535	multiple antimicrobial mechanisms	503:535	multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them	503:594	Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need.					
34164984	4	30	dep	%	996:996	arg1	photoinactivation					1042:1058	photoinactivation	1042:1058	photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively	1042:1146	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	6	31	theme	such	1603:1606	arg1	nanomaterials					1618:1630	such composite nanomaterials	1603:1630	such composite nanomaterials	1603:1630	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	1	32	theme	multidrug-resistant	208:226	arg1	pathogens					228:236	multidrug-resistant pathogens	208:236	multidrug-resistant pathogens attributed to the overuse of antibiotics	208:277	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	0	33	theme	Synergistic	0:10	arg1	Activity					56:63	Synergistic Photodynamic and Photothermal Antibacterial Activity	0:63	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.	0:171	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	3	34	theme	membrane	779:786	arg1	disruption					788:797	synergistic membrane disruption	767:797	synergistic membrane disruption	767:797	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	4	35	theme	BC/MoS2-CS	911:920	arg1	nanocomposite					922:934	The BC/MoS2-CS nanocomposite	907:934	The BC/MoS2-CS nanocomposite	907:934	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	36	theme	xenon	1183:1187	arg1	lamp					1189:1192	xenon lamp	1183:1192	xenon lamp	1183:1192	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	1	37	theme	pathogens	228:236	arg1	prevalence					194:203	prevalence	194:203	prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics	194:277	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	0	38	theme	Photothermal	29:40	arg1	Activity					56:63	Synergistic Photodynamic and Photothermal Antibacterial Activity	0:63	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.	0:171	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	5	39	theme	visible-light	1486:1498	arg1	illumination					1500:1511	visible-light illumination	1486:1511	visible-light illumination	1486:1511	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	5	40	theme	oxygen	1406:1411	arg1	species					1413:1419	reactive oxygen species	1397:1419	reactive oxygen species (photodynamic)	1397:1434	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	1	41	theme	microbes	330:337	arg1	transmission					314:325	the transmission	310:325	the transmission of microbes from contaminated surfaces to new hosts	310:377	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	0	42	theme	Light	153:157	arg1	Illumination					159:170	Visible Light Illumination	145:170	Visible Light Illumination	145:170	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	4	43	theme	log	1003:1005	arg1	%					996:996	99.998%	990:996	99.998% (4.7 log units)	990:1012	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	43	theme	log	1003:1005	arg1	units					1007:1011	4.7 log units	999:1011	4.7 log units	999:1011	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	5	44	dep	species	1413:1419	arg1	photodynamic					1422:1433	photodynamic	1422:1433	photodynamic	1422:1433	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	2	45	theme	multiple	503:510	arg1	mechanisms					526:535	multiple antimicrobial mechanisms	503:535	multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them	503:594	Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need.					
34164984	3	46	theme	synergistic	767:777	arg1	disruption					788:797	synergistic membrane disruption	767:797	synergistic membrane disruption	767:797	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	0	47	dep	In	68:69	arg1	Situ					71:74	Situ	71:74	Situ	71:74	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	0	48	theme	Antibacterial	42:54	arg1	Activity					56:63	Synergistic Photodynamic and Photothermal Antibacterial Activity	0:63	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.	0:171	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	1	49	theme	contaminated	344:355	arg1	surfaces					357:364	contaminated surfaces	344:364	contaminated surfaces	344:364	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	2	50	dep	need	611:614	arg1	functioning					487:497	functioning	487:497	functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them	487:594	Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need.					
34164984	3	51	theme	photodynamic	803:814	arg1	activities					847:856	photodynamic and photothermal antibacterial activities	803:856	photodynamic and photothermal antibacterial activities	803:856	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	0	52	theme	In	68:69	arg1	Materials					130:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	3	53	theme	photothermal	820:831	arg1	activities					847:856	photodynamic and photothermal antibacterial activities	803:856	photodynamic and photothermal antibacterial activities	803:856	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	2	54	theme	urgent	604:609	arg1	need					611:614	urgent need	604:614	urgent need	604:614	Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need.					
34164984	3	55	theme	termed	735:740	arg1	materials					724:732	grown bacterial cellulose/MoS2-chitosan nanocomposite materials	670:732	in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity	662:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	55	theme	termed	735:740	arg1	BC/MoS2-CS					742:751	termed BC/MoS2-CS	735:751	termed BC/MoS2-CS	735:751	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	56	theme	nanocomposite	710:722	arg1	materials					724:732	grown bacterial cellulose/MoS2-chitosan nanocomposite materials	670:732	in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity	662:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	56	theme	nanocomposite	710:722	arg1	BC/MoS2-CS					742:751	termed BC/MoS2-CS	735:751	termed BC/MoS2-CS	735:751	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	57	theme	grown	670:674	arg1	materials					724:732	grown bacterial cellulose/MoS2-chitosan nanocomposite materials	670:732	in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity	662:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	57	theme	grown	670:674	arg1	BC/MoS2-CS					742:751	termed BC/MoS2-CS	735:751	termed BC/MoS2-CS	735:751	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	6	58	theme	infection	1675:1683	arg1	applications					1693:1704	infection control applications	1675:1704	infection control applications	1675:1704	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	5	59	theme	bacterial	1309:1317	arg1	disruption					1328:1337	bacterial membrane disruption	1309:1337	bacterial membrane disruption	1309:1337	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	1	60	theme	new	369:371	arg1	hosts					373:377	new hosts	369:377	new hosts	369:377	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	3	61	theme	bacterial	676:684	arg1	materials					724:732	grown bacterial cellulose/MoS2-chitosan nanocomposite materials	670:732	in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity	662:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	61	theme	bacterial	676:684	arg1	BC/MoS2-CS					742:751	termed BC/MoS2-CS	735:751	termed BC/MoS2-CS	735:751	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	5	62	theme	membrane	1319:1326	arg1	disruption					1328:1337	bacterial membrane disruption	1309:1337	bacterial membrane disruption	1309:1337	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	5	63	theme	synergistic	1447:1457	arg1	inactivation					1468:1479	synergistic pathogen inactivation	1447:1479	synergistic pathogen inactivation upon visible-light illumination	1447:1511	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	3	64	theme	in	662:663	arg1	materials					724:732	grown bacterial cellulose/MoS2-chitosan nanocomposite materials	670:732	in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity	662:904	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	3	64	theme	in	662:663	arg1	BC/MoS2-CS					742:751	termed BC/MoS2-CS	735:751	termed BC/MoS2-CS	735:751	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	5	65	theme	reactive	1397:1404	arg1	species					1413:1419	reactive oxygen species	1397:1419	reactive oxygen species (photodynamic)	1397:1434	Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visible-light illumination.					
34164984	1	66	theme	antibiotics	267:277	arg1	overuse					256:262	the overuse	252:262	the overuse of antibiotics	252:277	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	0	67	theme	Grown	76:80	arg1	Materials					130:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials	68:138	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	4	68	theme	≥	1204:1204	arg1	nm					1210:1211	λ ≥ 420 nm	1202:1211	λ ≥ 420 nm	1202:1211	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	69	theme	visible-light	1155:1167	arg1	illumination					1169:1180	visible-light illumination	1155:1180	visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min)	1155:1224	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	3	70	theme	antibacterial	833:845	arg1	activities					847:856	photodynamic and photothermal antibacterial activities	803:856	photodynamic and photothermal antibacterial activities	803:856	Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS2-chitosan nanocomposite materials (termed BC/MoS2-CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity.					
34164984	6	71	theme	control	1685:1691	arg1	applications					1693:1704	infection control applications	1675:1704	infection control applications	1675:1704	No mammalian cell cytotoxicity was observed for the BC/MoS2-CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.					
34164984	0	72	with	Activity	56:63	arg1	Illumination					159:170	Visible Light Illumination	145:170	Visible Light Illumination	145:170	Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination.					
34164984	4	73	theme	coli	1089:1092	arg1	photoinactivation					1042:1058	photoinactivation	1042:1058	photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively	1042:1146	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	4	74	theme	aureus	1127:1132	arg1	photoinactivation					1042:1058	photoinactivation	1042:1058	photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively	1042:1146	The BC/MoS2-CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min).					
34164984	1	75	theme	infectious	280:289	arg1	threat					402:407	an ever-increasing threat	383:407	an ever-increasing threat to public health	383:424	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
34164984	1	75	theme	infectious	280:289	arg1	diseases					291:298	infectious diseases	280:298	infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts	280:377	Owing to the rise in prevalence of multidrug-resistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health.					
33046150	0	0	theme	Nile	96:99	arg1	tilapia					101:107	Nile tilapia	96:107	Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet	96:163	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	0	0	theme	Nile	96:99	arg1	niloticus					122:130	Oreochromis niloticus	110:130	Oreochromis niloticus	110:130	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	9	1	theme	acid	1644:1647	arg1	concentration					1601:1613	the concentration	1597:1613	the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1597:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	6	2	theme	up-regulated	1172:1183	arg1	genes					1185:1189	up-regulated genes	1172:1189	up-regulated genes relevant to β-oxidation	1172:1213	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	10	3	theme	causal	1816:1821	arg1	role					1823:1826	a possible causal role	1805:1826	a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish	1805:1901	Collectively, our results demonstrated a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish.					
33046150	7	4	theme	HC	1331:1332	arg1	group					1334:1338	the HC group	1327:1338	the HC group (P < 0·05)	1327:1349	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	7	4	theme	HC	1331:1332	arg1	0·05					1345:1348	P < 0·05	1341:1348	P < 0·05	1341:1348	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	7	5	theme	Higher	1241:1246	arg1	glycogen					1254:1261	Higher liver glycogen	1241:1261	Higher liver glycogen	1241:1261	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	1	6	theme	carbohydrate	338:349	arg1	ability					327:333	their poor utilisation ability	304:333	their poor utilisation ability of carbohydrate	304:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	9	7	theme	high-carbohydrate	1501:1517	arg1	diet					1519:1522	a high-carbohydrate diet	1499:1522	a high-carbohydrate diet	1499:1522	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	9	8	from	characteristics	1741:1755	arg1	fish					1760:1763	fish	1760:1763	fish	1760:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	1	9	theme	high-carbohydrate	168:184	arg1	diet					186:189	A high-carbohydrate diet	166:189	A high-carbohydrate diet	166:189	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	6	10	contain	had	1019:1021	arg1	group					1013:1017	the HCI group	1005:1017	the HCI group	1005:1017	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	6	10	contain	had	1019:1021	arg2	accumulation					1035:1046	lower lipid accumulation	1023:1046	lower lipid accumulation in liver	1023:1055	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	9	11	theme	fish	1652:1655	arg1	gut					1657:1659	fish gut	1652:1659	fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1652:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	4	12	dep	1·19	649:652	arg1	sd					655:656	sd 0·01	655:661	sd 0·01	655:661	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	7	13	theme	liver	1248:1252	arg1	glycogen					1254:1261	Higher liver glycogen	1241:1261	Higher liver glycogen	1241:1261	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	4	14	theme	carbohydrate	691:702	arg1	CON					705:707	35 % carbohydrate (CON)	686:708	35 % carbohydrate (CON)	686:708	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	6	15	theme	lipid	1029:1033	arg1	accumulation					1035:1046	lower lipid accumulation	1023:1046	lower lipid accumulation in liver	1023:1055	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	6	16	theme	lower	1023:1027	arg1	accumulation					1035:1046	lower lipid accumulation	1023:1046	lower lipid accumulation in liver	1023:1055	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	6	17	theme	expression	1070:1079	arg1	analyses					1081:1088	the gene expression analyses	1061:1088	the gene expression analyses	1061:1088	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	1	18	theme	growth	272:277	arg1	condition					279:287	the growth condition	268:287	the growth condition of fish due to their poor utilisation ability of carbohydrate	268:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	10	19	from	role	1823:1826	arg1	improvements					1864:1875	metabolic improvements	1854:1875	metabolic improvements induced by inulin in fish	1854:1901	Collectively, our results demonstrated a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish.					
33046150	6	20	theme	related	1145:1151	arg1	genes					1139:1143	genes	1139:1143	genes related to lipogenesis	1139:1166	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	4	21	theme	45	711:712	arg1	%					714:714	%	714:714	%	714:714	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	6	22	from	accumulation	1035:1046	arg1	liver					1051:1055	liver	1051:1055	liver	1051:1055	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	0	23	theme	Oreochromis	110:120	arg1	tilapia					101:107	Nile tilapia	96:107	Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet	96:163	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	0	23	theme	Oreochromis	110:120	arg1	niloticus					122:130	Oreochromis niloticus	110:130	Oreochromis niloticus	110:130	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	9	24	theme	pathogen	1698:1705	arg1	resistance					1707:1716	pathogen resistance	1698:1716	pathogen resistance	1698:1716	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	7	25	theme	HCI	1303:1305	arg1	group					1307:1311	the HCI group	1299:1311	the HCI group	1299:1311	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	8	26	theme	high-carbohydrate	1440:1456	arg1	diet					1458:1461	a high-carbohydrate diet	1438:1461	a high-carbohydrate diet	1438:1461	These results indicated that inulin could alleviate the metabolic syndrome induced by a high-carbohydrate diet.					
33046150	10	27	theme	gut	1836:1838	arg1	microbiome					1840:1849	the gut microbiome	1832:1849	the gut microbiome	1832:1849	Collectively, our results demonstrated a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish.					
33046150	4	28	theme	g/kg	760:763	arg1	inulin					765:770	5 g/kg inulin	758:770	5 g/kg inulin	758:770	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	4	29	theme	%	689:689	arg1	CON					705:707	35 % carbohydrate (CON)	686:708	35 % carbohydrate (CON)	686:708	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	9	30	theme	bacterial	1547:1555	arg1	composition					1557:1567	the intestinal bacterial composition	1532:1567	the intestinal bacterial composition	1532:1567	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	9	31	from	concentration	1601:1613	arg1	gut					1657:1659	fish gut	1652:1659	fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1652:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	1	32	from	impacts	257:263	arg1	condition					279:287	the growth condition	268:287	the growth condition of fish due to their poor utilisation ability of carbohydrate	268:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	10	33	theme	metabolic	1854:1862	arg1	improvements					1864:1875	metabolic improvements	1854:1875	metabolic improvements induced by inulin in fish	1854:1901	Collectively, our results demonstrated a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish.					
33046150	6	34	theme	inulin	1117:1122	arg1	addition					1105:1112	addition	1105:1112	addition of inulin	1105:1122	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	4	35	theme	35	686:687	arg1	%					689:689	%	689:689	%	689:689	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	2	36	theme	adverse	370:376	arg1	effects					378:384	the adverse effects	366:384	the adverse effects caused by a high-carbohydrate diet	366:419	How to reduce the adverse effects caused by a high-carbohydrate diet is important for the development of aquaculture.					
33046150	0	37	theme	metabolic	26:34	arg1	syndrome					36:43	adverse metabolic syndrome	18:43	adverse metabolic syndrome	18:43	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	7	38	located	found	1290:1294	arg2	tolerance					1275:1283	glucose tolerance	1267:1283	glucose tolerance	1267:1283	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	7	38	located	found	1290:1294	arg2	glycogen					1254:1261	Higher liver glycogen	1241:1261	Higher liver glycogen	1241:1261	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	7	38	located	found	1290:1294	arg1	group					1307:1311	the HCI group	1299:1311	the HCI group	1299:1311	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	1	39	theme	protein-sparing	207:221	arg1	effect					223:228	a protein-sparing effect	205:228	a protein-sparing effect	205:228	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	0	40	theme	adverse	18:24	arg1	syndrome					36:43	adverse metabolic syndrome	18:43	adverse metabolic syndrome	18:43	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	1	41	theme	fish	292:295	arg1	condition					279:287	the growth condition	268:287	the growth condition of fish due to their poor utilisation ability of carbohydrate	268:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	5	42	theme	survival	854:861	arg1	rate					863:866	the survival rate	850:866	the survival rate	850:866	The results showed that addition of inulin improved the survival rate when fish were challenged with Aeromonas hydrophila, indicating that inulin had an immunostimulatory effect.					
33046150	3	43	theme	metabolic	548:556	arg1	syndrome					558:565	the metabolic syndrome	544:565	the metabolic syndrome caused by a high-carbohydrate diet in fish	544:608	In the present study, we aimed to identify whether inulin could attenuate the metabolic syndrome caused by a high-carbohydrate diet in fish.					
33046150	9	44	theme	acetic	1618:1623	arg1	acid					1625:1628	acetic acid	1618:1628	acetic acid	1618:1628	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	6	45	theme	HC	995:996	arg1	group					998:1002	the HC group	991:1002	the HC group	991:1002	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	5	46	contain	had	944:946	arg1	inulin					937:942	inulin	937:942	inulin	937:942	The results showed that addition of inulin improved the survival rate when fish were challenged with Aeromonas hydrophila, indicating that inulin had an immunostimulatory effect.					
33046150	5	46	contain	had	944:946	arg2	effect					969:974	an immunostimulatory effect	948:974	an immunostimulatory effect	948:974	The results showed that addition of inulin improved the survival rate when fish were challenged with Aeromonas hydrophila, indicating that inulin had an immunostimulatory effect.					
33046150	8	47	theme	metabolic	1408:1416	arg1	syndrome					1418:1425	the metabolic syndrome	1404:1425	the metabolic syndrome induced by a high-carbohydrate diet	1404:1461	These results indicated that inulin could alleviate the metabolic syndrome induced by a high-carbohydrate diet.					
33046150	0	48	theme	high-carbohydrate	142:158	arg1	diet					160:163	high-carbohydrate diet	142:163	high-carbohydrate diet	142:163	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	5	49	theme	immunostimulatory	951:967	arg1	effect					969:974	an immunostimulatory effect	948:974	an immunostimulatory effect	948:974	The results showed that addition of inulin improved the survival rate when fish were challenged with Aeromonas hydrophila, indicating that inulin had an immunostimulatory effect.					
33046150	3	50	theme	high-carbohydrate	579:595	arg1	diet					597:600	a high-carbohydrate diet	577:600	a high-carbohydrate diet in fish	577:608	In the present study, we aimed to identify whether inulin could attenuate the metabolic syndrome caused by a high-carbohydrate diet in fish.					
33046150	3	51	from	diet	597:600	arg1	fish					605:608	fish	605:608	fish	605:608	In the present study, we aimed to identify whether inulin could attenuate the metabolic syndrome caused by a high-carbohydrate diet in fish.					
33046150	2	52	theme	high-carbohydrate	398:414	arg1	diet					416:419	a high-carbohydrate diet	396:419	a high-carbohydrate diet	396:419	How to reduce the adverse effects caused by a high-carbohydrate diet is important for the development of aquaculture.					
33046150	6	53	dep	significantly	1215:1227	arg1	P					1230:1230	P	1230:1230	P < 0·05	1230:1237	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	9	54	theme	propionic	1634:1642	arg1	acid					1644:1647	propionic acid	1634:1647	propionic acid	1634:1647	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	0	55	from	composition	81:91	arg1	tilapia					101:107	Nile tilapia	96:107	Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet	96:163	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	0	55	from	composition	81:91	arg1	niloticus					122:130	Oreochromis niloticus	110:130	Oreochromis niloticus	110:130	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	5	56	theme	Aeromonas	899:907	arg1	hydrophila					909:918	Aeromonas hydrophila	899:918	Aeromonas hydrophila	899:918	The results showed that addition of inulin improved the survival rate when fish were challenged with Aeromonas hydrophila, indicating that inulin had an immunostimulatory effect.					
33046150	9	57	theme	inulin	1489:1494	arg1	addition					1477:1484	addition	1477:1484	addition of inulin to a high-carbohydrate diet	1477:1522	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	4	58	theme	Oreochromis	625:635	arg1	tilapia					616:622	Nile tilapia	611:622	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g)	611:665	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	4	58	theme	Oreochromis	625:635	arg1	niloticus					637:645	Oreochromis niloticus	625:645	Oreochromis niloticus	625:645	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	2	59	theme	aquaculture	457:467	arg1	development					442:452	the development	438:452	the development of aquaculture	438:467	How to reduce the adverse effects caused by a high-carbohydrate diet is important for the development of aquaculture.					
33046150	0	60	theme	microbiota	70:79	arg1	composition					81:91	intestinal microbiota composition	59:91	intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet	59:163	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	4	61	theme	%	741:741	arg1	carbohydrate					743:754	45 % carbohydrate	738:754	45 % carbohydrate	738:754	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	4	61	theme	%	741:741	arg1	HCI					773:775	HCI	773:775	HCI	773:775	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	6	62	theme	relevant	1191:1198	arg1	genes					1185:1189	up-regulated genes	1172:1189	up-regulated genes relevant to β-oxidation	1172:1213	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	6	63	theme	HCI	1009:1011	arg1	group					1013:1017	the HCI group	1005:1017	the HCI group	1005:1017	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	7	64	theme	P	1341:1341	arg1	group					1334:1338	the HC group	1327:1338	the HC group (P < 0·05)	1327:1349	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	7	64	theme	P	1341:1341	arg1	0·05					1345:1348	P < 0·05	1341:1348	P < 0·05	1341:1348	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	1	65	theme	poor	310:313	arg1	ability					327:333	their poor utilisation ability	304:333	their poor utilisation ability of carbohydrate	304:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	9	66	theme	intestinal	1536:1545	arg1	composition					1557:1567	the intestinal bacterial composition	1532:1567	the intestinal bacterial composition	1532:1567	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	4	67	theme	45	738:739	arg1	%					741:741	%	741:741	%	741:741	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	10	68	theme	possible	1807:1814	arg1	role					1823:1826	a possible causal role	1805:1826	a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish	1805:1901	Collectively, our results demonstrated a possible causal role for the gut microbiome in metabolic improvements induced by inulin in fish.					
33046150	9	69	theme	metabolic	1731:1739	arg1	characteristics					1741:1755	metabolic characteristics	1731:1755	metabolic characteristics in fish	1731:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	1	70	theme	utilisation	315:325	arg1	ability					327:333	their poor utilisation ability	304:333	their poor utilisation ability of carbohydrate	304:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
33046150	4	71	dep	CON	705:707	arg1	diets					778:782	diets	778:782	diets	778:782	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	7	72	theme	glucose	1267:1273	arg1	tolerance					1275:1283	glucose tolerance	1267:1283	glucose tolerance	1267:1283	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	9	73	theme	acid	1625:1628	arg1	concentration					1601:1613	the concentration	1597:1613	the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1597:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	4	74	theme	%	714:714	arg1	HC					730:731	HC	730:731	HC	730:731	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	4	74	theme	%	714:714	arg1	carbohydrate					716:727	45 % carbohydrate	711:727	45 % carbohydrate (HC)	711:732	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	3	75	theme	present	477:483	arg1	study					485:489	the present study	473:489	the present study	473:489	In the present study, we aimed to identify whether inulin could attenuate the metabolic syndrome caused by a high-carbohydrate diet in fish.					
33046150	6	76	theme	gene	1065:1068	arg1	analyses					1081:1088	the gene expression analyses	1061:1088	the gene expression analyses	1061:1088	Compared with the HC group, the HCI group had lower lipid accumulation in liver and the gene expression analyses indicated that addition of inulin down-regulated genes related to lipogenesis and up-regulated genes relevant to β-oxidation significantly (P < 0·05).					
33046150	9	77	contain	have	1667:1670	arg1	gut					1657:1659	fish gut	1652:1659	fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1652:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	9	77	contain	have	1667:1670	arg2	potential					1676:1684	the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1672:1763	the potential to increase pathogen resistance and regulate metabolic characteristics in fish	1672:1763	Furthermore, addition of inulin to a high-carbohydrate diet changed the intestinal bacterial composition and significantly increased the concentration of acetic acid and propionic acid in fish gut which have the potential to increase pathogen resistance and regulate metabolic characteristics in fish.					
33046150	5	78	theme	inulin	834:839	arg1	addition					822:829	addition	822:829	addition of inulin	822:839	The results showed that addition of inulin improved the survival rate when fish were challenged with Aeromonas hydrophila, indicating that inulin had an immunostimulatory effect.					
33046150	4	79	theme	Nile	611:614	arg1	tilapia					616:622	Nile tilapia	611:622	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g)	611:665	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	4	79	theme	Nile	611:614	arg1	niloticus					637:645	Oreochromis niloticus	625:645	Oreochromis niloticus	625:645	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	4	79	theme	Nile	611:614	arg1	g					664:664	1·19 (sd 0·01) g	649:664	1·19 (sd 0·01) g	649:664	Nile tilapia (Oreochromis niloticus) (1·19 (sd 0·01) g) were supplied with 35 % carbohydrate (CON), 45 % carbohydrate (HC) and 45 % carbohydrate + 5 g/kg inulin (HCI) diets for 10 weeks.					
33046150	0	80	theme	intestinal	59:68	arg1	composition					81:91	intestinal microbiota composition	59:91	intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet	59:163	Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet.					
33046150	7	81	theme	<	1343:1343	arg1	group					1334:1338	the HC group	1327:1338	the HC group (P < 0·05)	1327:1349	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	7	81	theme	<	1343:1343	arg1	0·05					1345:1348	P < 0·05	1341:1348	P < 0·05	1341:1348	Higher liver glycogen and glucose tolerance were found in the HCI group compared with the HC group (P < 0·05).					
33046150	1	82	theme	negative	248:255	arg1	impacts					257:263	negative impacts	248:263	negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate	248:349	A high-carbohydrate diet could achieve a protein-sparing effect, but it may cause negative impacts on the growth condition of fish due to their poor utilisation ability of carbohydrate.					
34708264	2	0	theme	fungal	503:508	arg1	cocktails					520:528	the fungal enzymatic cocktails	499:528	the fungal enzymatic cocktails	499:528	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	1	1	theme	characterizing	351:364	arg1	cocktails					383:391	finely characterizing fungal enzymatic cocktails	344:391	finely characterizing fungal enzymatic cocktails	344:391	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	9	2	theme	cocktail	1658:1665	arg1	ability					1634:1640	the ability	1630:1640	the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation	1630:1734	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	7	3	from	results	1209:1215	arg1	substrates					1224:1233	CBS substrates	1220:1233	CBS substrates	1220:1233	However, the results on CBS substrates were more contrasted depending on the biomass tested.					
34708264	1	4	theme	different	247:255	arg1	conditions					270:279	different fermentation conditions	247:279	different fermentation conditions	247:279	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	6	5	located	observed	1101:1108	arg1	substrates					1054:1063	AZO and pNP-linked substrates	1035:1063	AZO and pNP-linked substrates	1035:1063	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	6	5	located	observed	1101:1108	arg2	profiles					1087:1094	different hydrolysis profiles	1066:1094	different hydrolysis profiles	1066:1094	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	1	6	theme	fungal	366:371	arg1	cocktails					383:391	finely characterizing fungal enzymatic cocktails	344:391	finely characterizing fungal enzymatic cocktails	344:391	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	1	7	theme	combinatory	152:162	arg1	approach					164:171	a combinatory approach	150:171	a combinatory approach	150:171	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	1	8	theme	fermentation	257:268	arg1	conditions					270:279	different fermentation conditions	247:279	different fermentation conditions	247:279	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	0	9	theme	fungal	99:104	arg1	cocktails					116:124	fungal enzymatic cocktails	99:124	fungal enzymatic cocktails	99:124	Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?					
34708264	1	10	theme	enzymatic	373:381	arg1	cocktails					383:391	finely characterizing fungal enzymatic cocktails	344:391	finely characterizing fungal enzymatic cocktails	344:391	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	9	11	theme	lignocellulosic	1700:1714	arg1	degradation					1724:1734	lignocellulosic biomass degradation	1700:1734	lignocellulosic biomass degradation	1700:1734	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	5	12	dep	RESULTS	876:882	arg1	showed					905:910	showed	905:910	showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails	905:1029	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	4	13	theme	monomers	782:789	arg1	quantification					754:767	a quantification	752:767	a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails	752:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	4	13	theme	monomers	782:789	arg1	efficiency					707:716	The saccharification efficiency	686:716	The saccharification efficiency of the cocktails	686:733	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	10	14	theme	simple	1827:1832	arg1	substrates					1846:1855	both simple and complex substrates	1822:1855	both simple and complex substrates	1822:1855	The saccharification assay proved to be essential to validate the data obtained from both simple and complex substrates.					
34708264	1	15	theme	cocktails	383:391	arg1	aim					337:339	the aim	333:339	the aim of finely characterizing fungal enzymatic cocktails	333:391	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	5	16	theme	same	1006:1009	arg1	cocktails					1021:1029	the same enzymatic cocktails	1002:1029	the same enzymatic cocktails	1002:1029	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	4	17	theme	sugar	776:780	arg1	monomers					782:789	the sugar monomers	772:789	the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails	772:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	6	18	theme	pNP-linked	1043:1052	arg1	substrates					1054:1063	AZO and pNP-linked substrates	1035:1063	AZO and pNP-linked substrates	1035:1063	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	8	19	theme	laccase	1340:1346	arg1	activities					1348:1357	laccase activities	1340:1357	laccase activities	1340:1357	Altogether, the results highlighted that assessing laccase activities and taking into account the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails.					
34708264	5	20	theme	enzymatic	1011:1019	arg1	cocktails					1021:1029	the same enzymatic cocktails	1002:1029	the same enzymatic cocktails	1002:1029	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	6	21	theme	fungi	1132:1136	arg1	species					1138:1144	the different fungi species	1118:1144	the different fungi species	1118:1144	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	0	22	theme	enzymatic	106:114	arg1	cocktails					116:124	fungal enzymatic cocktails	99:124	fungal enzymatic cocktails	99:124	Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?					
34708264	2	23	used	used	465:468	arg2	METHODS					394:400	METHODS	394:400	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS)	394:458	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	8	24	theme	biomass	1405:1411	arg1	complexity					1387:1396	the complexity	1383:1396	the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails	1383:1480	Altogether, the results highlighted that assessing laccase activities and taking into account the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails.					
34708264	2	25	dep	METHODS	394:400	arg1	assays					412:417	Enzymatic assays	402:417	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS)	394:458	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	2	26	theme	AZO	420:422	arg1	substrates					439:448	AZO and pNP-linked substrates	420:448	AZO and pNP-linked substrates	420:448	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	0	27	theme	analytical	10:19	arg1	approaches					21:30	analytical approaches	10:30	analytical approaches for better lignocellulosic biomass degradation	10:77	Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?					
34708264	8	28	theme	best	1457:1460	arg1	cocktails					1472:1480	the best enzymatic cocktails	1453:1480	the best enzymatic cocktails	1453:1480	Altogether, the results highlighted that assessing laccase activities and taking into account the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails.					
34708264	7	29	theme	CBS	1220:1222	arg1	substrates					1224:1233	CBS substrates	1220:1233	CBS substrates	1220:1233	However, the results on CBS substrates were more contrasted depending on the biomass tested.					
34708264	9	30	theme	complementary	1498:1510	arg1	experiments					1512:1522	The complementary experiments	1494:1522	The complementary experiments performed in this study	1494:1546	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	5	31	theme	CBS	983:985	arg1	substrates					987:996	the CBS substrates	979:996	the CBS substrates for the same enzymatic cocktails	979:1029	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	5	32	theme	striking	912:919	arg1	differences					921:931	striking differences	912:931	striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails	912:1029	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	1	33	theme	different	292:300	arg1	biomasses					318:326	different lignocellulosic biomasses	292:326	different lignocellulosic biomasses	292:326	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	1	34	from	conditions	270:279	arg1	cocktails					232:240	fungal enzymatic cocktails	215:240	fungal enzymatic cocktails from different fermentation conditions	215:279	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	1	34	from	conditions	270:279	arg1	efficiency					201:210	the efficiency	197:210	the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails	197:391	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	10	35	theme	saccharification	1741:1756	arg1	assay					1758:1762	The saccharification assay	1737:1762	The saccharification assay	1737:1762	The saccharification assay proved to be essential to validate the data obtained from both simple and complex substrates.					
34708264	10	35	theme	saccharification	1741:1756	arg1	essential					1777:1785	essential	1777:1785	essential	1777:1785	The saccharification assay proved to be essential to validate the data obtained from both simple and complex substrates.					
34708264	10	36	theme	complex	1838:1844	arg1	substrates					1846:1855	both simple and complex substrates	1822:1855	both simple and complex substrates	1822:1855	The saccharification assay proved to be essential to validate the data obtained from both simple and complex substrates.					
34708264	1	37	theme	lignocellulosic	302:316	arg1	biomasses					318:326	different lignocellulosic biomasses	292:326	different lignocellulosic biomasses	292:326	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	4	38	attach	released	791:798	arg1	biomasses					819:827	the different biomasses	805:827	the different biomasses	805:827	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	4	38	attach	released	791:798	arg2	monomers					782:789	the sugar monomers	772:789	the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails	772:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	5	39	theme	AZO	945:947	arg1	substrates					964:973	the AZO and pNP-linked substrates	941:973	the AZO and pNP-linked substrates	941:973	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	0	40	theme	lignocellulosic	43:57	arg1	degradation					67:77	better lignocellulosic biomass degradation	36:77	better lignocellulosic biomass degradation	36:77	Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?					
34708264	5	41	theme	pNP-linked	953:962	arg1	substrates					964:973	the AZO and pNP-linked substrates	941:973	the AZO and pNP-linked substrates	941:973	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	2	42	theme	laccase	558:564	arg1	activities					566:575	laccase activities	558:575	laccase activities	558:575	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	3	43	theme	CBS	669:671	arg1	biomass					660:666	complex biomass	652:666	complex biomass (CBS substrates)	652:683	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	3	43	theme	CBS	669:671	arg1	substrates					673:682	CBS substrates	669:682	CBS substrates	669:682	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	9	44	dep	CONCLUSION	1483:1492	arg1	showed					1548:1553	showed	1548:1553	showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation	1548:1734	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	6	45	theme	different	1066:1074	arg1	profiles					1087:1094	different hydrolysis profiles	1066:1094	different hydrolysis profiles	1066:1094	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	6	46	theme	AZO	1035:1037	arg1	substrates					1054:1063	AZO and pNP-linked substrates	1035:1063	AZO and pNP-linked substrates	1035:1063	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	3	47	theme	complex	652:658	arg1	biomass					660:666	complex biomass	652:666	complex biomass (CBS substrates)	652:683	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	3	47	theme	complex	652:658	arg1	substrates					673:682	CBS substrates	669:682	CBS substrates	669:682	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	9	48	theme	enzymatic	1648:1656	arg1	cocktail					1658:1665	an enzymatic cocktail	1645:1665	an enzymatic cocktail	1645:1665	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	1	49	from	efficiency	201:210	arg1	conditions					270:279	different fermentation conditions	247:279	different fermentation conditions	247:279	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	2	50	theme	pNP-linked	428:437	arg1	substrates					439:448	AZO and pNP-linked substrates	420:448	AZO and pNP-linked substrates	420:448	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	4	51	theme	enzymatic	855:863	arg1	cocktails					865:873	the enzymatic cocktails	851:873	the enzymatic cocktails	851:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	0	52	theme	biomass	59:65	arg1	degradation					67:77	better lignocellulosic biomass degradation	36:77	better lignocellulosic biomass degradation	36:77	Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?					
34708264	3	53	theme	new	607:609	arg1	range					611:615	a new range	605:615	a new range of chromogenic substrates based on complex biomass (CBS substrates)	605:683	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	9	54	theme	biomass	1716:1722	arg1	degradation					1724:1734	lignocellulosic biomass degradation	1700:1734	lignocellulosic biomass degradation	1700:1734	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	4	55	theme	cocktails	725:733	arg1	efficiency					707:716	The saccharification efficiency	686:716	The saccharification efficiency of the cocktails	686:733	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	4	55	theme	cocktails	725:733	arg1	quantification					754:767	a quantification	752:767	a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails	752:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	8	56	theme	enzymatic	1462:1470	arg1	cocktails					1472:1480	the best enzymatic cocktails	1453:1480	the best enzymatic cocktails	1453:1480	Altogether, the results highlighted that assessing laccase activities and taking into account the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails.					
34708264	3	57	theme	substrates	632:641	arg1	range					611:615	a new range	605:615	a new range of chromogenic substrates based on complex biomass (CBS substrates)	605:683	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	6	58	link	pNP-linked	1043:1052	arg1	substrates					1054:1063	AZO and pNP-linked substrates	1035:1063	AZO and pNP-linked substrates	1035:1063	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	2	59	link	pNP-linked	428:437	arg1	substrates					439:448	AZO and pNP-linked substrates	420:448	AZO and pNP-linked substrates	420:448	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	4	60	theme	different	809:817	arg1	biomasses					819:827	the different biomasses	805:827	the different biomasses	805:827	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	9	61	theme	different	1560:1568	arg1	approaches					1570:1579	different approaches	1560:1579	different approaches	1560:1579	CONCLUSION The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation.					
34708264	1	62	theme	fungal	215:220	arg1	cocktails					232:240	fungal enzymatic cocktails	215:240	fungal enzymatic cocktails from different fermentation conditions	215:279	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	5	63	link	pNP-linked	953:962	arg1	substrates					964:973	the AZO and pNP-linked substrates	941:973	the AZO and pNP-linked substrates	941:973	RESULTS The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails.					
34708264	2	64	dep	assays	412:417	arg1	substrates					439:448	AZO and pNP-linked substrates	420:448	AZO and pNP-linked substrates	420:448	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	2	64	dep	assays	412:417	arg1	ABTS					454:457	ABTS	454:457	ABTS	454:457	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	1	65	theme	enzymatic	222:230	arg1	cocktails					232:240	fungal enzymatic cocktails	215:240	fungal enzymatic cocktails from different fermentation conditions	215:279	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	2	66	theme	cocktails	520:528	arg1	composition					484:494	the composition	480:494	the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities	480:575	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	6	67	theme	hydrolysis	1076:1085	arg1	profiles					1087:1094	different hydrolysis profiles	1066:1094	different hydrolysis profiles	1066:1094	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	3	68	theme	chromogenic	620:630	arg1	substrates					632:641	chromogenic substrates	620:641	chromogenic substrates based on complex biomass (CBS substrates)	620:683	Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates).					
34708264	2	69	theme	Enzymatic	402:410	arg1	assays					412:417	Enzymatic assays	402:417	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS)	394:458	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	6	70	theme	different	1122:1130	arg1	species					1138:1144	the different fungi species	1118:1144	the different fungi species	1118:1144	On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient.					
34708264	1	71	theme	cocktails	232:240	arg1	efficiency					201:210	the efficiency	197:210	the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails	197:391	PURPOSE In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.					
34708264	2	72	theme	enzymatic	510:518	arg1	cocktails					520:528	the fungal enzymatic cocktails	499:528	the fungal enzymatic cocktails	499:528	METHODS Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities.					
34708264	0	73	dep	Combining	0:8	arg1	way					82:84	a way	80:84	a way of improving fungal enzymatic cocktails	80:124	Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?					
34708264	4	74	theme	saccharification	690:705	arg1	efficiency					707:716	The saccharification efficiency	686:716	The saccharification efficiency of the cocktails	686:733	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	4	74	theme	saccharification	690:705	arg1	quantification					754:767	a quantification	752:767	a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails	752:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34708264	4	75	with	incubation	835:844	arg1	cocktails					865:873	the enzymatic cocktails	851:873	the enzymatic cocktails	851:873	The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.					
34156547	0	0	from	formation	15:23	arg1	implants					50:57	orthopedic implants	39:57	orthopedic implants	39:57	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	5	1	theme	osteoinductive	737:750	arg1	substance					770:778	osteoinductive and antibacterial substance	737:778	substance	770:778	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	2	2	theme	VRSA	395:398	arg1	infections					424:433	MRSA and VRSA associated bone implant infections	386:433	infections	424:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	0	3	theme	thin	71:74	arg1	layer					76:80	melittin thin layer	62:80	melittin thin layer on chitosan/bioactive glass/vancomycin coatings	62:128	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	5	4	theme	cells	695:699	arg1	proliferation					672:684	the proliferation	668:684	the proliferation of MC3T3 cells	668:699	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	0	5	theme	chitosan/bioactive	85:102	arg1	coatings					121:128	chitosan/bioactive glass/vancomycin coatings	85:128	chitosan/bioactive glass/vancomycin coatings	85:128	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	5	6	theme	antibacterial	756:768	arg1	substance					770:778	osteoinductive and antibacterial substance	737:778	substance	770:778	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	7	7	theme	bone	1100:1103	arg1	implant					1105:1111	a bone implant	1098:1111	a bone implant coating because of its anti-infective properties	1098:1160	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	7	7	theme	bone	1100:1103	arg1	coating					1075:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	2	8	theme	synergistic	325:335	arg1	effects					337:343	the potential synergistic effects	311:343	the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections	311:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	2	9	theme	potential	315:323	arg1	effects					337:343	the potential synergistic effects	311:343	the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections	311:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	6	10	theme	VRSA	1004:1007	arg1	bacteria					1009:1016	the whole VRSA bacteria	994:1016	the whole VRSA bacteria	994:1016	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	11	theme	VRSA	924:927	arg1	bacteria					929:936	both planktonic and adherent MRSA and VRSA bacteria	886:936	bacteria	929:936	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	12	theme	Composite	816:824	arg1	coatings					826:833	Composite coatings	816:833	Composite coatings having combined vancomycin and Melittin	816:873	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	0	13	theme	glass/vancomycin	104:119	arg1	coatings					121:128	chitosan/bioactive glass/vancomycin coatings	85:128	chitosan/bioactive glass/vancomycin coatings	85:128	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	4	14	theme	composite	577:585	arg1	coatings					587:594	The composite coatings	573:594	The composite coatings	573:594	The composite coatings were coated by Melittin through drop casting technique.					
34156547	5	15	theme	appropriate	715:725	arg1	option					727:732	an appropriate option	712:732	an appropriate option	712:732	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	1	16	dep	Methicillin-resistant	131:151	arg1	VRSA					219:222	VRSA	219:222	VRSA	219:222	Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants.					
34156547	1	16	dep	Methicillin-resistant	131:151	arg1	MRSA					210:213	MRSA	210:213	MRSA	210:213	Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants.					
34156547	1	16	dep	Methicillin-resistant	131:151	arg1	bacteria					200:207	bacteria	200:207	bacteria	200:207	Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants.					
34156547	3	17	theme	etched	527:532	arg1	substrates					543:552	hydrothermally etched titanium substrates	512:552	hydrothermally etched titanium substrates	512:552	Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method.					
34156547	3	18	theme	nanoparticles/vancomycin	461:484	arg1	composites					486:495	Chitosan/bioactive glass nanoparticles/vancomycin composites	436:495	Chitosan/bioactive glass nanoparticles/vancomycin composites	436:495	Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method.					
34156547	3	19	theme	Chitosan/bioactive	436:453	arg1	composites					486:495	Chitosan/bioactive glass nanoparticles/vancomycin composites	436:495	Chitosan/bioactive glass nanoparticles/vancomycin composites	436:495	Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method.					
34156547	6	20	theme	adherent	906:913	arg1	MRSA					915:918	both planktonic and adherent MRSA and VRSA bacteria	886:936	MRSA	915:918	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	2	21	theme	Melittin	348:355	arg1	effects					337:343	the potential synergistic effects	311:343	the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections	311:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	2	22	theme	implant	416:422	arg1	infections					424:433	MRSA and VRSA associated bone implant infections	386:433	infections	424:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	7	23	theme	glass/vancomycin/Melittin	1049:1073	arg1	implant					1105:1111	a bone implant	1098:1111	a bone implant coating because of its anti-infective properties	1098:1160	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	7	23	theme	glass/vancomycin/Melittin	1049:1073	arg1	coating					1075:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	5	24	theme	implants	806:813	arg1	coatings					783:790	coatings	783:790	coatings of orthopedic implants	783:813	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	5	25	theme	MC3T3	689:693	arg1	cells					695:699	MC3T3 cells	689:699	MC3T3 cells	689:699	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	0	26	from	layer	76:80	arg1	coatings					121:128	chitosan/bioactive glass/vancomycin coatings	85:128	chitosan/bioactive glass/vancomycin coatings	85:128	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	3	27	theme	casting	557:563	arg1	method					565:570	casting method	557:570	casting method	557:570	Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method.					
34156547	6	28	contain	having	835:840	arg2	vancomycin					851:860	combined vancomycin	842:860	combined vancomycin	842:860	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	28	contain	having	835:840	arg2	Melittin					866:873	Melittin	866:873	Melittin	866:873	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	28	contain	having	835:840	arg1	coatings					826:833	Composite coatings	816:833	Composite coatings having combined vancomycin and Melittin	816:873	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	7	29	used	used	1090:1093	arg2	implant					1105:1111	a bone implant	1098:1111	a bone implant coating because of its anti-infective properties	1098:1160	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	7	29	used	used	1090:1093	arg2	coating					1075:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	3	30	theme	titanium	534:541	arg1	substrates					543:552	hydrothermally etched titanium substrates	512:552	hydrothermally etched titanium substrates	512:552	Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method.					
34156547	0	31	theme	biofilm	28:34	arg1	formation					15:23	the formation	11:23	the formation of biofilm on orthopedic implants	11:57	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	6	32	theme	planktonic	891:900	arg1	MRSA					915:918	both planktonic and adherent MRSA and VRSA bacteria	886:936	MRSA	915:918	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	3	33	theme	glass	455:459	arg1	composites					486:495	Chitosan/bioactive glass nanoparticles/vancomycin composites	436:495	Chitosan/bioactive glass nanoparticles/vancomycin composites	436:495	Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method.					
34156547	5	34	from	substance	770:778	arg1	coatings					783:790	coatings	783:790	coatings of orthopedic implants	783:813	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	6	35	theme	them	974:977	arg1	one					967:969	one	967:969	one	967:969	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	35	theme	them	974:977	arg1	them					974:977	them	974:977	them	974:977	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	36	theme	whole	998:1002	arg1	bacteria					1009:1016	the whole VRSA bacteria	994:1016	the whole VRSA bacteria	994:1016	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	2	37	theme	MRSA	386:389	arg1	infections					424:433	MRSA and VRSA associated bone implant infections	386:433	infections	424:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	0	38	theme	orthopedic	39:48	arg1	implants					50:57	orthopedic implants	39:57	orthopedic implants	39:57	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	4	39	theme	casting	633:639	arg1	technique					641:649	drop casting technique	628:649	drop casting technique	628:649	The composite coatings were coated by Melittin through drop casting technique.					
34156547	6	40	contain	containing	956:965	arg1	coatings					947:954	coatings	947:954	coatings containing one of them	947:977	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	40	contain	containing	956:965	arg2	them					974:977	them	974:977	them	974:977	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	6	40	contain	containing	956:965	arg2	one					967:969	one	967:969	one	967:969	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	4	41	theme	drop	628:631	arg1	technique					641:649	drop casting technique	628:649	drop casting technique	628:649	The composite coatings were coated by Melittin through drop casting technique.					
34156547	7	42	theme	anti-infective	1136:1149	arg1	properties					1151:1160	its anti-infective properties	1132:1160	its anti-infective properties	1132:1160	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	7	43	theme	chitosan/bioactive	1030:1047	arg1	implant					1105:1111	a bone implant	1098:1111	a bone implant coating because of its anti-infective properties	1098:1160	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	7	43	theme	chitosan/bioactive	1030:1047	arg1	coating					1075:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	chitosan/bioactive glass/vancomycin/Melittin coating	1030:1081	Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.					
34156547	5	44	theme	orthopedic	795:804	arg1	implants					806:813	orthopedic implants	795:813	orthopedic implants	795:813	Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants.					
34156547	2	45	theme	vancomycin	361:370	arg1	effects					337:343	the potential synergistic effects	311:343	the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections	311:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	1	46	theme	bone	265:268	arg1	implants					270:277	bone implants	265:277	bone implants	265:277	Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants.					
34156547	0	47	theme	melittin	62:69	arg1	layer					76:80	melittin thin layer	62:80	melittin thin layer on chitosan/bioactive glass/vancomycin coatings	62:128	Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings.					
34156547	2	48	theme	bone	411:414	arg1	infections					424:433	MRSA and VRSA associated bone implant infections	386:433	infections	424:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
34156547	6	49	theme	combined	842:849	arg1	vancomycin					851:860	combined vancomycin	842:860	combined vancomycin	842:860	Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria.					
34156547	2	50	theme	associated	400:409	arg1	infections					424:433	MRSA and VRSA associated bone implant infections	386:433	infections	424:433	This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections.					
33910731	8	0	theme	composite	1097:1105	arg1	hydrogel					1107:1114	the multi-functional composite hydrogel	1076:1114	the multi-functional composite hydrogel	1076:1114	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	1	1	theme	mechanical	167:176	arg1	adaptability					178:189	mechanical adaptability	167:189	mechanical adaptability	167:189	It is still a challenge to integrate high sensitivity, mechanical adaptability, and self-powered properties for hydrogels.					
33910731	0	2	theme	cellulose	29:37	arg1	nanofibrils					39:49	Nature-inspired self-powered cellulose nanofibrils	0:49	Nature-inspired self-powered cellulose nanofibrils	0:49	Nature-inspired self-powered cellulose nanofibrils hydrogels with high sensitivity and mechanical adaptability.					
33910731	4	3	theme	hydrogel	564:571	arg1	network					549:555	the network	545:555	the network of the hydrogel	545:571	Meanwhile, the nanoclay could be well dispersed and immobilized in the network of the hydrogel, thus improving mechanical adaptability of the hydrogel for curved and dynamic surfaces.					
33910731	8	4	theme	multi-functional	1080:1095	arg1	hydrogel					1107:1114	the multi-functional composite hydrogel	1076:1114	the multi-functional composite hydrogel	1076:1114	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	8	5	theme	wearable	1178:1185	arg1	devices					1187:1193	wearable devices	1178:1193	wearable devices	1178:1193	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	6	6	theme	pressure	851:858	arg1	changes					860:866	tiny pressure changes	846:866	tiny pressure changes	846:866	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	7	7	theme	mV	1060:1061	arg1	output					1033:1038	an output voltage	1030:1046	an output voltage of up to 78 mV	1030:1061	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	8	8	contain	have	1120:1123	arg2	applications					1131:1142	giant applications	1125:1142	giant applications	1125:1142	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	8	8	contain	have	1120:1123	arg1	hydrogel					1107:1114	the multi-functional composite hydrogel	1076:1114	the multi-functional composite hydrogel	1076:1114	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	3	9	theme	CNFs	363:366	arg1	chains					376:381	The CNFs and PVA chains	359:381	The CNFs and PVA chains	359:381	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	6	10	theme	tiny	846:849	arg1	changes					860:866	tiny pressure changes	846:866	tiny pressure changes	846:866	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	6	11	dep	changes	860:866	arg1	enabling					869:876	enabling	869:876	enabling recognition of the complicated sounding and handwriting	869:932	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	1	12	theme	high	149:152	arg1	sensitivity					154:164	high sensitivity	149:164	high sensitivity	149:164	It is still a challenge to integrate high sensitivity, mechanical adaptability, and self-powered properties for hydrogels.					
33910731	8	13	theme	giant	1125:1129	arg1	applications					1131:1142	giant applications	1125:1142	giant applications	1125:1142	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	8	14	theme	artificial	1147:1156	arg1	skins					1169:1173	artificial electronic skins	1147:1173	artificial electronic skins	1147:1173	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	4	15	theme	dynamic	644:650	arg1	surfaces					652:659	curved and dynamic surfaces	633:659	surfaces	652:659	Meanwhile, the nanoclay could be well dispersed and immobilized in the network of the hydrogel, thus improving mechanical adaptability of the hydrogel for curved and dynamic surfaces.					
33910731	7	16	contain	possessed	976:984	arg1	hydrogel					967:974	the composite hydrogel	953:974	the composite hydrogel	953:974	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	7	16	contain	possessed	976:984	arg2	property					999:1006	self-powered property	986:1006	self-powered property	986:1006	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	5	17	theme	S	750:750	arg1	conductivity					731:742	high conductivity	726:742	high conductivity (6.67 S m-1)	726:755	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	5	17	theme	S	750:750	arg1	m-1					752:754	6.67 S m-1	745:754	6.67 S m-1	745:754	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	0	18	theme	self-powered	16:27	arg1	nanofibrils					39:49	Nature-inspired self-powered cellulose nanofibrils	0:49	Nature-inspired self-powered cellulose nanofibrils	0:49	Nature-inspired self-powered cellulose nanofibrils hydrogels with high sensitivity and mechanical adaptability.					
33910731	6	19	theme	high	826:829	arg1	sensitivity					831:841	high sensitivity	826:841	high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting	826:932	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	5	20	theme	high	726:729	arg1	conductivity					731:742	high conductivity	726:742	high conductivity (6.67 S m-1)	726:755	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	5	20	theme	high	726:729	arg1	m-1					752:754	6.67 S m-1	745:754	6.67 S m-1	745:754	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	1	21	theme	self-powered	196:207	arg1	properties					209:218	self-powered properties	196:218	self-powered properties	196:218	It is still a challenge to integrate high sensitivity, mechanical adaptability, and self-powered properties for hydrogels.					
33910731	0	22	theme	Nature-inspired	0:14	arg1	nanofibrils					39:49	Nature-inspired self-powered cellulose nanofibrils	0:49	Nature-inspired self-powered cellulose nanofibrils	0:49	Nature-inspired self-powered cellulose nanofibrils hydrogels with high sensitivity and mechanical adaptability.					
33910731	8	23	theme	electronic	1158:1167	arg1	skins					1169:1173	artificial electronic skins	1147:1173	artificial electronic skins	1147:1173	In summary, the multi-functional composite hydrogel may have giant applications in artificial electronic skins or wearable devices.					
33910731	6	24	theme	handwriting	922:932	arg1	recognition					878:888	recognition	878:888	recognition of the complicated sounding and handwriting	878:932	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	2	25	theme	natural	308:314	arg1	nanoclay					316:323	natural nanoclay	308:323	natural nanoclay	308:323	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	4	26	theme	hydrogel	620:627	arg1	adaptability					600:611	mechanical adaptability	589:611	mechanical adaptability of the hydrogel for curved and dynamic surfaces	589:659	Meanwhile, the nanoclay could be well dispersed and immobilized in the network of the hydrogel, thus improving mechanical adaptability of the hydrogel for curved and dynamic surfaces.					
33910731	3	27	theme	PVA	372:374	arg1	chains					376:381	The CNFs and PVA chains	359:381	The CNFs and PVA chains	359:381	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	7	28	theme	voltage	1040:1046	arg1	output					1033:1038	an output voltage	1030:1046	an output voltage of up to 78 mV	1030:1061	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	7	29	dep	78	1057:1058	arg1	to					1054:1055	to	1054:1055	to	1054:1055	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	3	30	theme	composite	458:466	arg1	hydrogel					468:475	a high mechanical composite hydrogel	440:475	a high mechanical composite hydrogel	440:475	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	3	31	theme	double	401:406	arg1	structure					416:424	a double network structure	399:424	a double network structure	399:424	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	6	32	theme	sounding	909:916	arg1	recognition					878:888	recognition	878:888	recognition of the complicated sounding and handwriting	878:932	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	4	33	theme	curved	633:638	arg1	surfaces					652:659	curved and dynamic surfaces	633:659	surfaces	652:659	Meanwhile, the nanoclay could be well dispersed and immobilized in the network of the hydrogel, thus improving mechanical adaptability of the hydrogel for curved and dynamic surfaces.					
33910731	3	34	theme	network	408:414	arg1	structure					416:424	a double network structure	399:424	a double network structure	399:424	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	6	35	theme	complicated	897:907	arg1	sounding					909:916	the complicated sounding	893:916	the complicated sounding	893:916	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	6	36	theme	composite	797:805	arg1	hydrogel					807:814	the composite hydrogel	793:814	the composite hydrogel	793:814	Therefore, the composite hydrogel exhibited high sensitivity to tiny pressure changes, enabling recognition of the complicated sounding and handwriting.					
33910731	3	37	theme	high	442:445	arg1	hydrogel					468:475	a high mechanical composite hydrogel	440:475	a high mechanical composite hydrogel	440:475	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	7	38	theme	self-powered	986:997	arg1	property					999:1006	self-powered property	986:1006	self-powered property	986:1006	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	0	39	theme	high	66:69	arg1	sensitivity					71:81	high sensitivity	66:81	high sensitivity	66:81	Nature-inspired self-powered cellulose nanofibrils hydrogels with high sensitivity and mechanical adaptability.					
33910731	7	40	theme	composite	957:965	arg1	hydrogel					967:974	the composite hydrogel	953:974	the composite hydrogel	953:974	More importantly, the composite hydrogel possessed self-powered property, which could generate an output voltage of up to 78 mV.					
33910731	4	41	theme	mechanical	589:598	arg1	adaptability					600:611	mechanical adaptability	589:611	mechanical adaptability of the hydrogel for curved and dynamic surfaces	589:659	Meanwhile, the nanoclay could be well dispersed and immobilized in the network of the hydrogel, thus improving mechanical adaptability of the hydrogel for curved and dynamic surfaces.					
33910731	5	42	theme	conductive	676:685	arg1	Al3+					693:696	Al3+	693:696	Al3+	693:696	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	5	42	theme	conductive	676:685	arg1	ions					687:690	the conductive ions	672:690	the conductive ions (Al3+)	672:697	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	2	43	theme	alcohol	276:282	arg1	hydrogel					290:297	a conductive polyvinyl alcohol (PVA) hydrogel	253:297	a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs)	253:356	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	2	44	theme	cellulose	329:337	arg1	CNFs					352:355	CNFs	352:355	CNFs	352:355	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	2	44	theme	cellulose	329:337	arg1	nanofibrils					339:349	cellulose nanofibrils	329:349	cellulose nanofibrils (CNFs)	329:356	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	0	45	theme	mechanical	87:96	arg1	adaptability					98:109	mechanical adaptability	87:109	mechanical adaptability	87:109	Nature-inspired self-powered cellulose nanofibrils hydrogels with high sensitivity and mechanical adaptability.					
33910731	2	46	theme	polyvinyl	266:274	arg1	PVA					285:287	PVA	285:287	PVA	285:287	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	2	46	theme	polyvinyl	266:274	arg1	alcohol					276:282	conductive polyvinyl alcohol	255:282	a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs)	253:356	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	3	47	theme	mechanical	447:456	arg1	hydrogel					468:475	a high mechanical composite hydrogel	440:475	a high mechanical composite hydrogel	440:475	The CNFs and PVA chains could construct a double network structure, resulting in a high mechanical composite hydrogel.					
33910731	2	48	theme	conductive	255:264	arg1	PVA					285:287	PVA	285:287	PVA	285:287	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	2	48	theme	conductive	255:264	arg1	alcohol					276:282	conductive polyvinyl alcohol	255:282	a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs)	253:356	Herein, we report a conductive polyvinyl alcohol (PVA) hydrogel based on natural nanoclay and cellulose nanofibrils (CNFs).					
33910731	5	49	theme	gauge	761:765	arg1	factor					767:772	gauge factor	761:772	gauge factor (1.17)	761:779	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
33910731	5	49	theme	gauge	761:765	arg1	1.17					775:778	1.17	775:778	1.17	775:778	Moreover, the conductive ions (Al3+) imparted the hydrogel with high conductivity (6.67 S m-1) and gauge factor (1.17).					
32285264	0	0	theme	starch	90:95	arg1	residue					97:103	fermented cassava starch residue	72:103	fermented cassava starch residue	72:103	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	6	1	contain	had	791:793	arg1	native					784:789	native	784:789	native	784:789	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	6	1	contain	had	791:793	arg2	gain					810:813	greater weight gain	795:813	greater weight gain	795:813	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	8	2	theme	native	1000:1005	arg1	7 months					984:991	7 months	984:991	7 months of the native	984:1005	Blood triglyceride concentration sampled at 7 months of the native was higher than that of the crossbred; in contrast, blood cholesterol of the crossbred tended to be higher than that of the native.					
32285264	11	3	theme	growth	1602:1607	arg1	rate					1609:1612	similar growth rate	1594:1612	similar growth rate	1594:1612	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	3	4	theme	%	488:488	arg1	BW					490:491	1.50% BW	484:491	1.50% BW of animal	484:501	Yeast, molasses, and urea-fermented cassava starch residue was offered at 1.50% BW of animal.					
32285264	1	5	theme	fermented	251:259	arg1	residue					276:282	fermented cassava starch residue	251:282	fermented cassava starch residue	251:282	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	0	6	theme	cassava	82:88	arg1	residue					97:103	fermented cassava starch residue	72:103	fermented cassava starch residue	72:103	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	6	7	theme	greater	795:801	arg1	gain					810:813	greater weight gain	795:813	greater weight gain	795:813	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	1	8	theme	Angus	164:168	arg1	performance					141:151	growth performance	134:151	growth performance of Lowline Angus crossbred with Thai native cattle	134:202	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	1	9	theme	cassava	261:267	arg1	residue					276:282	fermented cassava starch residue	251:282	fermented cassava starch residue	251:282	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	11	10	theme	feed	1512:1515	arg1	supplementation					1517:1531	low-quality feed supplementation	1500:1531	low-quality feed supplementation	1500:1531	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	10	11	theme	fermentation	1363:1374	arg1	end-products					1376:1387	ruminal fermentation end-products	1355:1387	ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids)	1355:1431	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	11	12	theme	grazing	1477:1483	arg1	condition					1485:1493	grazing condition	1477:1493	grazing condition with low-quality feed supplementation	1477:1531	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	2	13	theme	grass	296:300	arg1	sub-fields					302:311	Three Ruzi grass sub-fields	285:311	Three Ruzi grass sub-fields	285:311	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	1	14	theme	starch	269:274	arg1	residue					276:282	fermented cassava starch residue	251:282	fermented cassava starch residue	251:282	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	6	15	from	month	769:773	arg1	different					726:734	different	726:734	different	726:734	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	10	16	theme	ruminal	1355:1361	arg1	end-products					1376:1387	ruminal fermentation end-products	1355:1387	ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids)	1355:1431	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	2	17	theme	Ruzi	291:294	arg1	sub-fields					302:311	Three Ruzi grass sub-fields	285:311	Three Ruzi grass sub-fields	285:311	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	6	18	theme	cattle	697:702	arg1	rate					684:687	Overall growth rate	669:687	Overall growth rate of beef cattle	669:702	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	1	19	theme	residue	276:282	arg1	supplementation					232:246	supplementation	232:246	supplementation of fermented cassava starch residue	232:282	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	7	20	theme	Average	835:841	arg1	gain					849:852	Average daily gain	835:852	Average daily gain	835:852	Average daily gain was 353 and 345 g for Thai native and its crossbred with Lowline Angus, respectively.					
32285264	5	21	theme	temperature-humidity	608:627	arg1	highest					639:645	highest	639:645	highest	639:645	The temperature-humidity index was highest (75.6-84.6) in April.					
32285264	5	21	theme	temperature-humidity	608:627	arg1	index					629:633	The temperature-humidity index	604:633	The temperature-humidity index	604:633	The temperature-humidity index was highest (75.6-84.6) in April.					
32285264	8	22	theme	triglyceride	946:957	arg1	concentration					959:971	Blood triglyceride concentration	940:971	Blood triglyceride concentration sampled at 7 months of the native	940:1005	Blood triglyceride concentration sampled at 7 months of the native was higher than that of the crossbred; in contrast, blood cholesterol of the crossbred tended to be higher than that of the native.					
32285264	6	23	theme	beef	692:695	arg1	cattle					697:702	beef cattle	692:702	beef cattle	692:702	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	10	24	from	population	1448:1457	arg1	similar					1325:1331	similar	1325:1331	similar	1325:1331	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	24	from	population	1448:1457	arg1	native					1295:1300	native	1295:1300	native	1295:1300	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	11	25	theme	similar	1594:1600	arg1	rate					1609:1612	similar growth rate	1594:1612	similar growth rate	1594:1612	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	9	26	theme	Other	1139:1143	arg1	glucose					1173:1179	glucose	1173:1179	glucose	1173:1179	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	globulin					1250:1257	globulin	1250:1257	globulin	1250:1257	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	cholesterol					1182:1192	cholesterol	1182:1192	cholesterol	1182:1192	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	protein					1228:1234	total protein	1222:1234	total protein	1222:1234	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	metabolites					1151:1161	Other blood metabolites	1139:1161	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin	1139:1257	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	nitrogen					1200:1207	urea nitrogen	1195:1207	urea nitrogen	1195:1207	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	albumin					1237:1243	albumin	1237:1243	albumin	1237:1243	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	26	theme	Other	1139:1143	arg1	creatinine					1210:1219	creatinine	1210:1219	creatinine	1210:1219	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	10	27	theme	ammonia	1390:1396	arg1	nitrogen					1398:1405	ammonia nitrogen	1390:1405	ammonia nitrogen	1390:1405	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	28	from	end-products	1376:1387	arg1	similar					1325:1331	similar	1325:1331	similar	1325:1331	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	28	from	end-products	1376:1387	arg1	native					1295:1300	native	1295:1300	native	1295:1300	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	9	29	theme	blood	1145:1149	arg1	glucose					1173:1179	glucose	1173:1179	glucose	1173:1179	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	globulin					1250:1257	globulin	1250:1257	globulin	1250:1257	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	cholesterol					1182:1192	cholesterol	1182:1192	cholesterol	1182:1192	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	protein					1228:1234	total protein	1222:1234	total protein	1222:1234	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	metabolites					1151:1161	Other blood metabolites	1139:1161	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin	1139:1257	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	nitrogen					1200:1207	urea nitrogen	1195:1207	urea nitrogen	1195:1207	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	albumin					1237:1243	albumin	1237:1243	albumin	1237:1243	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	9	29	theme	blood	1145:1149	arg1	creatinine					1210:1219	creatinine	1210:1219	creatinine	1210:1219	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	1	30	theme	Thai	185:188	arg1	cattle					197:202	Thai native cattle	185:202	Thai native cattle	185:202	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	0	31	theme	Nutritional	0:10	arg1	status					12:17	Nutritional status	0:17	Nutritional status of grazing Lowline Angus	0:42	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	11	32	with	condition	1485:1493	arg1	supplementation					1517:1531	low-quality feed supplementation	1500:1531	low-quality feed supplementation	1500:1531	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	6	33	theme	weight	803:808	arg1	gain					810:813	greater weight gain	795:813	greater weight gain	795:813	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	6	34	theme	first	763:767	arg1	month					769:773	the first month	759:773	the first month	759:773	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	10	35	theme	volatile	1411:1418	arg1	acids					1426:1430	volatile fatty acids	1411:1430	volatile fatty acids	1411:1430	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	6	36	theme	growth	677:682	arg1	rate					684:687	Overall growth rate	669:687	Overall growth rate of beef cattle	669:702	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	1	37	theme	native	190:195	arg1	cattle					197:202	Thai native cattle	185:202	Thai native cattle	185:202	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	0	38	theme	grazing	22:28	arg1	status					12:17	Nutritional status	0:17	Nutritional status of grazing Lowline Angus	0:42	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	4	39	theme	fecal	525:529	arg1	composition					531:541	fecal composition	525:541	fecal composition	525:541	Blood metabolite and fecal composition were determined for health and nutritional stage assessment.					
32285264	6	40	theme	Overall	669:675	arg1	rate					684:687	Overall growth rate	669:687	Overall growth rate of beef cattle	669:702	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	3	41	theme	starch	454:459	arg1	residue					461:467	urea-fermented cassava starch residue	431:467	urea-fermented cassava starch residue	431:467	Yeast, molasses, and urea-fermented cassava starch residue was offered at 1.50% BW of animal.					
32285264	2	42	theme	cattle	348:353	arg1	heads					334:338	twenty heads	327:338	twenty heads of beef cattle	327:353	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	11	43	contain	had	1590:1592	arg1	cattle					1551:1556	Thai native beef cattle	1534:1556	Thai native beef cattle	1534:1556	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	11	43	contain	had	1590:1592	arg2	health					1615:1620	health	1615:1620	health	1615:1620	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	11	43	contain	had	1590:1592	arg2	rate					1609:1612	similar growth rate	1594:1612	similar growth rate	1594:1612	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	11	43	contain	had	1590:1592	arg1	Angus					1574:1578	Angus	1574:1578	Angus	1574:1578	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	11	43	contain	had	1590:1592	arg2	status					1639:1644	nutritional status	1627:1644	nutritional status	1627:1644	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	8	44	theme	Blood	940:944	arg1	concentration					959:971	Blood triglyceride concentration	940:971	Blood triglyceride concentration sampled at 7 months of the native	940:1005	Blood triglyceride concentration sampled at 7 months of the native was higher than that of the crossbred; in contrast, blood cholesterol of the crossbred tended to be higher than that of the native.					
32285264	7	45	theme	daily	843:847	arg1	gain					849:852	Average daily gain	835:852	Average daily gain	835:852	Average daily gain was 353 and 345 g for Thai native and its crossbred with Lowline Angus, respectively.					
32285264	11	46	theme	nutritional	1627:1637	arg1	status					1639:1644	nutritional status	1627:1644	nutritional status	1627:1644	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	3	47	theme	urea-fermented	431:444	arg1	residue					461:467	urea-fermented cassava starch residue	431:467	urea-fermented cassava starch residue	431:467	Yeast, molasses, and urea-fermented cassava starch residue was offered at 1.50% BW of animal.					
32285264	5	48	dep	highest	639:645	arg1	75.6-84.6					648:656	75.6-84.6	648:656	75.6-84.6	648:656	The temperature-humidity index was highest (75.6-84.6) in April.					
32285264	4	49	theme	Blood	504:508	arg1	metabolite					510:519	Blood metabolite	504:519	Blood metabolite	504:519	Blood metabolite and fecal composition were determined for health and nutritional stage assessment.					
32285264	10	50	theme	fatty	1420:1424	arg1	acids					1426:1430	volatile fatty acids	1411:1430	volatile fatty acids	1411:1430	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	0	51	dep	crossbred	44:52	arg1	supplemented					54:65	supplemented	54:65	crossbred supplemented with fermented cassava starch residue	44:103	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	11	52	theme	Thai	1534:1537	arg1	cattle					1551:1556	Thai native beef cattle	1534:1556	Thai native beef cattle	1534:1556	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	1	53	theme	grazing	212:218	arg1	system					220:225	a grazing system	210:225	a grazing system with supplementation of fermented cassava starch residue	210:282	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	8	54	theme	blood	1059:1063	arg1	cholesterol					1065:1075	blood cholesterol	1059:1075	blood cholesterol of the crossbred	1059:1092	Blood triglyceride concentration sampled at 7 months of the native was higher than that of the crossbred; in contrast, blood cholesterol of the crossbred tended to be higher than that of the native.					
32285264	8	55	theme	the	1080:1082	arg1	cholesterol					1065:1075	blood cholesterol	1059:1075	blood cholesterol of the crossbred	1059:1092	Blood triglyceride concentration sampled at 7 months of the native was higher than that of the crossbred; in contrast, blood cholesterol of the crossbred tended to be higher than that of the native.					
32285264	11	56	theme	native	1539:1544	arg1	cattle					1551:1556	Thai native beef cattle	1534:1556	Thai native beef cattle	1534:1556	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	2	57	theme	beef	343:346	arg1	cattle					348:353	beef cattle	343:353	beef cattle	343:353	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	10	58	dep	end-products	1376:1387	arg1	nitrogen					1398:1405	ammonia nitrogen	1390:1405	ammonia nitrogen	1390:1405	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	58	dep	end-products	1376:1387	arg1	acids					1426:1430	volatile fatty acids	1411:1430	volatile fatty acids	1411:1430	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	6	59	from	different	726:734	arg1	month					769:773	the first month	759:773	the first month	759:773	Overall growth rate of beef cattle was not significantly different among breeds except in the first month when the native had greater weight gain than the crossbred.					
32285264	9	60	theme	urea	1195:1198	arg1	nitrogen					1200:1207	urea nitrogen	1195:1207	urea nitrogen	1195:1207	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	10	61	theme	fecal	1336:1340	arg1	composition					1342:1352	fecal composition	1336:1352	fecal composition	1336:1352	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	9	62	theme	total	1222:1226	arg1	protein					1228:1234	total protein	1222:1234	total protein	1222:1234	Other blood metabolites including glucose, cholesterol, urea nitrogen, creatinine, total protein, albumin, and globulin were comparable between breeds.					
32285264	10	63	from	composition	1342:1352	arg1	similar					1325:1331	similar	1325:1331	similar	1325:1331	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	63	from	composition	1342:1352	arg1	native					1295:1300	native	1295:1300	native	1295:1300	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	64	theme	microbial	1438:1446	arg1	population					1448:1457	microbial population	1438:1457	microbial population	1438:1457	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	11	65	theme	low-quality	1500:1510	arg1	supplementation					1517:1531	low-quality feed supplementation	1500:1531	low-quality feed supplementation	1500:1531	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	2	66	from	February	385:392	arg1	experiment					369:378	a 7-month experiment	359:378	a 7-month experiment from February to August 2017	359:407	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	10	67	from	similar	1325:1331	arg1	end-products					1376:1387	ruminal fermentation end-products	1355:1387	ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids)	1355:1431	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	67	from	similar	1325:1331	arg1	population					1448:1457	microbial population	1438:1457	microbial population	1438:1457	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	10	67	from	similar	1325:1331	arg1	composition					1342:1352	fecal composition	1336:1352	fecal composition	1336:1352	The native and the crossbred were similar in fecal composition, ruminal fermentation end-products (ammonia nitrogen and volatile fatty acids), and microbial population.					
32285264	11	68	theme	beef	1546:1549	arg1	cattle					1551:1556	Thai native beef cattle	1534:1556	Thai native beef cattle	1534:1556	Therefore, under grazing condition with low-quality feed supplementation, Thai native beef cattle and its Lowline Angus crossbred had similar growth rate, health, and nutritional status.					
32285264	1	69	theme	growth	134:139	arg1	performance					141:151	growth performance	134:151	growth performance of Lowline Angus crossbred with Thai native cattle	134:202	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	2	70	used	used	318:321	arg2	sub-fields					302:311	Three Ruzi grass sub-fields	285:311	Three Ruzi grass sub-fields	285:311	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	4	71	theme	stage	586:590	arg1	assessment					592:601	nutritional stage assessment	574:601	nutritional stage assessment	574:601	Blood metabolite and fecal composition were determined for health and nutritional stage assessment.					
32285264	0	72	theme	fermented	72:80	arg1	residue					97:103	fermented cassava starch residue	72:103	fermented cassava starch residue	72:103	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	3	73	theme	cassava	446:452	arg1	residue					461:467	urea-fermented cassava starch residue	431:467	urea-fermented cassava starch residue	431:467	Yeast, molasses, and urea-fermented cassava starch residue was offered at 1.50% BW of animal.					
32285264	0	74	dep	status	12:17	arg1	Angus					38:42	Angus	38:42	Angus	38:42	Nutritional status of grazing Lowline Angus crossbred supplemented with fermented cassava starch residue.					
32285264	2	75	theme	7-month	361:367	arg1	experiment					369:378	a 7-month experiment	359:378	a 7-month experiment from February to August 2017	359:407	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	4	76	theme	nutritional	574:584	arg1	assessment					592:601	nutritional stage assessment	574:601	nutritional stage assessment	574:601	Blood metabolite and fecal composition were determined for health and nutritional stage assessment.					
32285264	2	77	dep	February	385:392	arg1	to					394:395	to	394:395	to	394:395	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	2	77	dep	February	385:392	arg1	August					397:402	August	397:402	August	397:402	Three Ruzi grass sub-fields were used for twenty heads of beef cattle for a 7-month experiment from February to August 2017.					
32285264	1	78	with	system	220:225	arg1	supplementation					232:246	supplementation	232:246	supplementation of fermented cassava starch residue	232:282	This study aimed to compare growth performance of Lowline Angus crossbred with Thai native cattle under a grazing system with supplementation of fermented cassava starch residue.					
32285264	3	79	theme	animal	496:501	arg1	BW					490:491	1.50% BW	484:491	1.50% BW of animal	484:501	Yeast, molasses, and urea-fermented cassava starch residue was offered at 1.50% BW of animal.					
34841463	3	0	theme	Mtb	692:694	arg1	H37Rv					702:706	Mtb ATCC® H37Rv	692:706	Mtb ATCC® H37Rv	692:706	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	9	1	theme	promising	1556:1564	arg1	composite					1566:1574	a promising composite	1554:1574	a promising composite against strains Mtb H37Rv and multidrug resistant (MDR-TB)	1554:1633	It is concluded that CS-EMB forms a promising composite against strains Mtb H37Rv and multidrug resistant (MDR-TB).					
34841463	7	2	theme	Mtb	1401:1403	arg1	wall					1410:1413	the Mtb cell wall	1397:1413	the Mtb cell wall	1397:1413	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	1	3	theme	promising	157:165	arg1	Chitosan					138:145	Chitosan	138:145	Chitosan (CS)	138:150	Chitosan (CS) is a promising biopolymer and has been tested as a complement to the action and compensation of toxicity presented by anti-tuberculosis drugs.					
34841463	1	3	theme	promising	157:165	arg1	biopolymer					167:176	a promising biopolymer	155:176	a promising biopolymer	155:176	Chitosan (CS) is a promising biopolymer and has been tested as a complement to the action and compensation of toxicity presented by anti-tuberculosis drugs.					
34841463	6	4	theme	Drug-polymer	902:913	arg1	interactions					915:926	Drug-polymer interactions	902:926	Drug-polymer interactions that occur through the H bridges	902:959	Drug-polymer interactions that occur through the H bridges were confirmed by physical-chemical analyses.					
34841463	7	5	from	EMB	1216:1218	arg1	bacilli					1288:1294	the bacilli	1284:1294	the bacilli observed by TEM	1284:1310	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	5	from	EMB	1216:1218	arg1	strains					1241:1247	the H37Rv and MDR strains	1223:1247	strains	1241:1247	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	6	theme	antibacterial	1102:1114	arg1	concentration					1145:1157	minimum inhibitory concentration	1126:1157	minimum inhibitory concentration	1126:1157	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	6	theme	antibacterial	1102:1114	arg1	activity					1116:1123	The antibacterial activity	1098:1123	The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound	1098:1181	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	1	7	theme	toxicity	248:255	arg1	compensation					232:243	compensation	232:243	compensation	232:243	Chitosan (CS) is a promising biopolymer and has been tested as a complement to the action and compensation of toxicity presented by anti-tuberculosis drugs.					
34841463	1	7	theme	toxicity	248:255	arg1	action					221:226	action	221:226	action	221:226	Chitosan (CS) is a promising biopolymer and has been tested as a complement to the action and compensation of toxicity presented by anti-tuberculosis drugs.					
34841463	0	8	theme	Mycobacterium	110:122	arg1	tuberculosis					124:135	Mycobacterium tuberculosis	110:135	Mycobacterium tuberculosis	110:135	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	3	9	theme	reference	674:682	arg1	strains					684:690	reference strains	674:690	reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR)	674:736	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	2	10	theme	cytotoxic	434:442	arg1	activity					444:451	its antimicrobial and cytotoxic activity	412:451	its antimicrobial and cytotoxic activity	412:451	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	12	11	contain	has	1847:1849	arg1	•					1825:1825	•	1825:1825	• The CS-EMB compound	1825:1845	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	12	11	contain	has	1847:1849	arg2	potential					1861:1869	promising potential	1851:1869	promising potential for the development of a new drug to fight tuberculosis	1851:1925	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	4	12	theme	In	739:740	arg1	tests					761:765	In vitro cytotoxicity tests	739:765	In vitro cytotoxicity tests	739:765	In vitro cytotoxicity tests were performed on Raw 264.7.					
34841463	12	13	theme	drug	1900:1903	arg1	development					1879:1889	the development	1875:1889	the development of a new drug to fight tuberculosis	1875:1925	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	6	14	theme	physical-chemical	979:995	arg1	analyses					997:1004	physical-chemical analyses	979:1004	physical-chemical analyses	979:1004	Drug-polymer interactions that occur through the H bridges were confirmed by physical-chemical analyses.					
34841463	7	15	theme	greater	1191:1197	arg1	%					1189:1189	50%	1187:1189	50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM	1187:1310	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	16	theme	cell	1405:1408	arg1	wall					1410:1413	the Mtb cell wall	1397:1413	the Mtb cell wall	1397:1413	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	2	17	theme	possible	548:555	arg1	action					575:580	possible antimycobacterial action	548:580	possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb)	548:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	4	18	dep	In	739:740	arg1	vitro					742:746	vitro	742:746	vitro	742:746	In vitro cytotoxicity tests were performed on Raw 264.7.					
34841463	10	19	from	effects	1708:1714	arg1	cells					1746:1750	Mtb cells	1742:1750	Mtb cells	1742:1750	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	2	20	dep	studied	312:318	arg1	explore					404:410	explore	404:410	explore	404:410	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	12	21	theme	fight	1908:1912	arg1	tuberculosis					1914:1925	fight tuberculosis	1908:1925	fight tuberculosis	1908:1925	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	12	22	theme	CS-EMB	1831:1836	arg1	compound					1838:1845	The CS-EMB compound	1827:1845	• The CS-EMB compound	1825:1845	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	7	23	contain	has	1344:1346	arg1	compound					1335:1342	the CS-EMB compound	1324:1342	the CS-EMB compound	1324:1342	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	23	contain	has	1344:1346	arg2	action					1363:1368	a bactericidal action	1348:1368	a bactericidal action	1348:1368	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	24	dep	proved	1312:1317	arg1	%					1189:1189	50%	1187:1189	50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM	1187:1310	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	4	25	theme	cytotoxicity	748:759	arg1	tests					761:765	In vitro cytotoxicity tests	739:765	In vitro cytotoxicity tests	739:765	In vitro cytotoxicity tests were performed on Raw 264.7.					
34841463	11	26	theme	multidrug-resistant	1789:1807	arg1	strain					1818:1823	a multidrug-resistant clinical strain	1787:1823	a multidrug-resistant clinical strain	1787:1823	• CS-EMB antimicrobial activity in a multidrug-resistant clinical strain.					
34841463	5	27	theme	morphological	823:835	arg1	analyses					877:884	morphological, ultrastructural, and physical-chemical analyses	823:884	morphological, ultrastructural, and physical-chemical analyses	823:884	For the studied compounds, morphological, ultrastructural, and physical-chemical analyses were performed.					
34841463	2	28	theme	electron	473:480	arg1	microscopy					482:491	transmission electron microscopy	460:491	transmission electron microscopy (TEM)	460:497	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	28	theme	electron	473:480	arg1	TEM					494:496	TEM	494:496	TEM	494:496	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	3	29	theme	ATCC®	696:700	arg1	H37Rv					702:706	Mtb ATCC® H37Rv	692:706	Mtb ATCC® H37Rv	692:706	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	2	30	theme	Mycobacterium	591:603	arg1	Mtb					619:621	Mtb	619:621	Mtb	619:621	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	30	theme	Mycobacterium	591:603	arg1	tuberculosis					605:616	Mycobacterium tuberculosis	591:616	Mycobacterium tuberculosis (Mtb)	591:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	7	31	theme	compound	1174:1181	arg1	concentration					1145:1157	minimum inhibitory concentration	1126:1157	minimum inhibitory concentration	1126:1157	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	31	theme	compound	1174:1181	arg1	activity					1116:1123	The antibacterial activity	1098:1123	The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound	1098:1181	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	32	theme	CS-EMB	1167:1172	arg1	compound					1174:1181	the CS-EMB compound	1163:1181	the CS-EMB compound	1163:1181	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	2	33	from	action	575:580	arg1	Mtb					619:621	Mtb	619:621	Mtb	619:621	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	33	from	action	575:580	arg1	tuberculosis					605:616	Mycobacterium tuberculosis	591:616	Mycobacterium tuberculosis (Mtb)	591:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	34	dep	explore	404:410	arg1	examine					503:509	examine	503:509	to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb)	500:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	7	35	theme	MDR	1237:1239	arg1	strains					1241:1247	the H37Rv and MDR strains	1223:1247	strains	1241:1247	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	5	36	theme	ultrastructural	838:852	arg1	analyses					877:884	morphological, ultrastructural, and physical-chemical analyses	823:884	morphological, ultrastructural, and physical-chemical analyses	823:884	For the studied compounds, morphological, ultrastructural, and physical-chemical analyses were performed.					
34841463	0	37	theme	Antimycobacterial	0:16	arg1	compound					18:25	Antimycobacterial compound	0:25	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.	0:136	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	1	38	theme	anti-tuberculosis	270:286	arg1	drugs					288:292	anti-tuberculosis drugs	270:292	anti-tuberculosis drugs	270:292	Chitosan (CS) is a promising biopolymer and has been tested as a complement to the action and compensation of toxicity presented by anti-tuberculosis drugs.					
34841463	2	39	theme	CS	585:586	arg1	action					575:580	possible antimycobacterial action	548:580	possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb)	548:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	9	40	theme	Mtb	1592:1594	arg1	H37Rv					1596:1600	Mtb H37Rv	1592:1600	Mtb H37Rv	1592:1600	It is concluded that CS-EMB forms a promising composite against strains Mtb H37Rv and multidrug resistant (MDR-TB).					
34841463	11	41	theme	clinical	1809:1816	arg1	strain					1818:1823	a multidrug-resistant clinical strain	1787:1823	a multidrug-resistant clinical strain	1787:1823	• CS-EMB antimicrobial activity in a multidrug-resistant clinical strain.					
34841463	0	42	theme	chitosan	30:37	arg1	compound					18:25	Antimycobacterial compound	0:25	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.	0:136	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	10	43	theme	compound	1730:1737	arg1	effects					1708:1714	the effects	1704:1714	the effects of the CS-EMB compound on Mtb cells	1704:1750	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	7	44	theme	bactericidal	1350:1361	arg1	action					1363:1368	a bactericidal action	1348:1368	a bactericidal action	1348:1368	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	3	45	dep	strains	684:690	arg1	MDR					733:735	MDR	733:735	MDR	733:735	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	3	45	dep	strains	684:690	arg1	H37Rv					702:706	Mtb ATCC® H37Rv	692:706	Mtb ATCC® H37Rv	692:706	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	3	45	dep	strains	684:690	arg1	resistant					722:730	resistant	722:730	resistant	722:730	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	12	46	dep	•	1825:1825	arg1	compound					1838:1845	The CS-EMB compound	1827:1845	• The CS-EMB compound	1825:1845	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	7	47	dep	pH.	1094:1096	arg1	proved					1312:1317	proved	1312:1317	was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall	1183:1413	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	2	48	theme	present	299:305	arg1	work					307:310	The present work	295:310	The present work	295:310	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	12	49	theme	new	1896:1898	arg1	drug					1900:1903	a new drug	1894:1903	a new drug to fight tuberculosis	1894:1925	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	5	50	theme	studied	804:810	arg1	compounds					812:820	the studied compounds	800:820	the studied compounds	800:820	For the studied compounds, morphological, ultrastructural, and physical-chemical analyses were performed.					
34841463	0	51	theme	ethambutol	43:52	arg1	compound					18:25	Antimycobacterial compound	0:25	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.	0:136	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	0	52	theme	biological	71:80	arg1	evaluation					82:91	ultrastructural biological evaluation	55:91	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.	0:136	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	7	53	theme	6.5-7.5	1047:1053	arg1	pHs					1040:1042	pHs	1040:1042	pHs of 6.5-7.5	1040:1053	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	6	54	theme	H	951:951	arg1	bridges					953:959	the H bridges	947:959	the H bridges	947:959	Drug-polymer interactions that occur through the H bridges were confirmed by physical-chemical analyses.					
34841463	7	55	theme	minimum	1126:1132	arg1	concentration					1145:1157	minimum inhibitory concentration	1126:1157	minimum inhibitory concentration	1126:1157	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	55	theme	minimum	1126:1132	arg1	activity					1116:1123	The antibacterial activity	1098:1123	The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound	1098:1181	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	11	56	theme	antimicrobial	1761:1773	arg1	activity					1775:1782	antimicrobial activity	1761:1782	antimicrobial activity	1761:1782	• CS-EMB antimicrobial activity in a multidrug-resistant clinical strain.					
34841463	0	57	from	evaluation	82:91	arg1	vitro					96:100	vitro	96:100	vitro	96:100	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	7	58	theme	ultrastructural	1257:1271	arg1	changes					1273:1279	the ultrastructural changes	1253:1279	the ultrastructural changes in the bacilli observed by TEM	1253:1310	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	2	59	theme	antimicrobial	416:428	arg1	activity					444:451	its antimicrobial and cytotoxic activity	412:451	its antimicrobial and cytotoxic activity	412:451	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	10	60	theme	points•	1639:1645	arg1	study					1651:1655	Key points• Our study	1635:1655	Key points• Our study	1635:1655	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	10	60	theme	points•	1639:1645	arg1	first					1669:1673	first	1669:1673	first	1669:1673	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	1	61	dep	action	221:226	arg1	the					217:219	the	217:219	the	217:219	Chitosan (CS) is a promising biopolymer and has been tested as a complement to the action and compensation of toxicity presented by anti-tuberculosis drugs.					
34841463	7	62	theme	CS-EMB	1328:1333	arg1	compound					1335:1342	the CS-EMB compound	1324:1342	the CS-EMB compound	1324:1342	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	2	63	theme	CS	343:344	arg1	compound					382:389	a compound	380:389	a compound (CS-EMB)	380:398	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	63	theme	CS	343:344	arg1	effect					333:338	the adjuvant effect	320:338	the adjuvant effect of CS	320:344	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	7	64	theme	inhibitory	1134:1143	arg1	concentration					1145:1157	minimum inhibitory concentration	1126:1157	minimum inhibitory concentration	1126:1157	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	64	theme	inhibitory	1134:1143	arg1	activity					1116:1123	The antibacterial activity	1098:1123	The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound	1098:1181	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	65	from	changes	1273:1279	arg1	bacilli					1288:1294	the bacilli	1284:1294	the bacilli observed by TEM	1284:1310	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	65	from	changes	1273:1279	arg1	strains					1241:1247	the H37Rv and MDR strains	1223:1247	strains	1241:1247	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	2	66	theme	antimycobacterial	557:573	arg1	action					575:580	possible antimycobacterial action	548:580	possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb)	548:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	7	67	theme	H37Rv	1227:1231	arg1	strains					1241:1247	the H37Rv and MDR strains	1223:1247	strains	1241:1247	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	10	68	theme	Mtb	1742:1744	arg1	cells					1746:1750	Mtb cells	1742:1750	Mtb cells	1742:1750	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	7	69	theme	CS-EMB	1011:1016	arg1	stable					1030:1035	stable	1030:1035	stable	1030:1035	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	69	theme	CS-EMB	1011:1016	arg1	compound					1018:1025	The CS-EMB compound	1007:1025	The CS-EMB compound	1007:1025	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	10	70	theme	Key	1635:1637	arg1	points•					1639:1645	Key points•	1635:1645	Key points• Our study	1635:1655	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	2	71	theme	ultracellular	511:523	arg1	changes					525:531	ultracellular changes	511:531	ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb)	511:622	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	3	72	theme	Antimycobacterial	625:641	arg1	activities					643:652	Antimycobacterial activities	625:652	Antimycobacterial activities	625:652	Antimycobacterial activities were tested against reference strains Mtb ATCC® H37Rv and multidrug resistant (MDR).					
34841463	8	73	theme	CS-EMB	1436:1441	arg1	higher					1447:1452	higher	1447:1452	higher	1447:1452	The cytotoxicity of CS-EMB was higher than that of isolated EMB, IC50 279, and 176 μg/mL, respectively.					
34841463	8	73	theme	CS-EMB	1436:1441	arg1	cytotoxicity					1420:1431	The cytotoxicity	1416:1431	The cytotoxicity of CS-EMB	1416:1441	The cytotoxicity of CS-EMB was higher than that of isolated EMB, IC50 279, and 176 μg/mL, respectively.					
34841463	2	74	theme	adjuvant	324:331	arg1	compound					382:389	a compound	380:389	a compound (CS-EMB)	380:398	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	74	theme	adjuvant	324:331	arg1	effect					333:338	the adjuvant effect	320:338	the adjuvant effect of CS	320:344	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	75	theme	transmission	460:471	arg1	microscopy					482:491	transmission electron microscopy	460:491	transmission electron microscopy (TEM)	460:497	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	75	theme	transmission	460:471	arg1	TEM					494:496	TEM	494:496	TEM	494:496	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	0	76	dep	compound	18:25	arg1	evaluation					82:91	ultrastructural biological evaluation	55:91	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.	0:136	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	8	77	theme	isolated	1467:1474	arg1	EMB					1476:1478	isolated EMB	1467:1478	isolated EMB	1467:1478	The cytotoxicity of CS-EMB was higher than that of isolated EMB, IC50 279, and 176 μg/mL, respectively.					
34841463	5	78	theme	physical-chemical	859:875	arg1	analyses					877:884	morphological, ultrastructural, and physical-chemical analyses	823:884	morphological, ultrastructural, and physical-chemical analyses	823:884	For the studied compounds, morphological, ultrastructural, and physical-chemical analyses were performed.					
34841463	7	79	from	pHs	1040:1042	arg1	stable					1030:1035	stable	1030:1035	stable	1030:1035	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	7	79	from	pHs	1040:1042	arg1	compound					1018:1025	The CS-EMB compound	1007:1025	The CS-EMB compound	1007:1025	The CS-EMB compound is stable at pHs of 6.5-7.5, allowing its release at physiological pH. The antibacterial activity (minimum inhibitory concentration) of the CS-EMB compound was 50% greater than that of the EMB in the H37Rv and MDR strains and the ultrastructural changes in the bacilli observed by TEM proved that the CS-EMB compound has a bactericidal action, allowing it to break down the Mtb cell wall.					
34841463	0	80	theme	ultrastructural	55:69	arg1	evaluation					82:91	ultrastructural biological evaluation	55:91	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.	0:136	Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.					
34841463	10	81	theme	CS-EMB	1723:1728	arg1	compound					1730:1737	the CS-EMB compound	1719:1737	the CS-EMB compound	1719:1737	Key points• Our study will be the first to observe ultrastructurally the effects of the CS-EMB compound on Mtb cells.					
34841463	12	82	theme	promising	1851:1859	arg1	potential					1861:1869	promising potential	1851:1869	promising potential for the development of a new drug to fight tuberculosis	1851:1925	• The CS-EMB compound has promising potential for the development of a new drug to fight tuberculosis.					
34841463	2	83	theme	drug	355:358	arg1	EMB					372:374	EMB	372:374	EMB	372:374	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	2	83	theme	drug	355:358	arg1	ethambutol					360:369	the drug ethambutol	351:369	the drug ethambutol (EMB)	351:375	The present work studied the adjuvant effect of CS with the drug ethambutol (EMB) as a compound (CS-EMB), to explore its antimicrobial and cytotoxic activity, using transmission electron microscopy (TEM), to examine ultracellular changes that represent possible antimycobacterial action of CS on Mycobacterium tuberculosis (Mtb).					
34841463	9	84	dep	strains	1584:1590	arg1	MDR-TB					1627:1632	MDR-TB	1627:1632	MDR-TB	1627:1632	It is concluded that CS-EMB forms a promising composite against strains Mtb H37Rv and multidrug resistant (MDR-TB).					
34841463	9	84	dep	strains	1584:1590	arg1	H37Rv					1596:1600	Mtb H37Rv	1592:1600	Mtb H37Rv	1592:1600	It is concluded that CS-EMB forms a promising composite against strains Mtb H37Rv and multidrug resistant (MDR-TB).					
34841463	9	84	dep	strains	1584:1590	arg1	resistant					1616:1624	resistant	1616:1624	resistant	1616:1624	It is concluded that CS-EMB forms a promising composite against strains Mtb H37Rv and multidrug resistant (MDR-TB).					
33895175	10	0	theme	degradation	1342:1352	arg1	performance					1354:1364	good catalytic degradation performance	1327:1364	good catalytic degradation performance for bisphenol A	1327:1380	In addition, Cu(II) loaded Fe3O4-ATP/EDTA/CS also showed good catalytic degradation performance for bisphenol A.					
33895175	1	1	theme	porous	156:161	arg1	structure					177:185	a unique porous layered chain structure	147:185	a unique porous layered chain structure	147:185	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	4	2	from	embedded	448:455	arg1	beads					478:482	chitosan (CS) gel beads	460:482	chitosan (CS) gel beads	460:482	Then, Fe3O4-ATP nanoparticles were embedded in chitosan (CS) gel beads, and glutaraldehyde was used for cross-linking and curing.					
33895175	0	3	theme	Ni	117:118	arg1	removal					86:92	the removal	82:92	the removal of Cu(II), Pb(II), and Ni(II)	82:122	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	7	4	theme	maximum	856:862	arg1	capacity					875:882	The maximum adsorption capacity	852:882	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II)	852:957	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	7	4	theme	maximum	856:862	arg1	368.32					962:967	368.32	962:967	368.32	962:967	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	1	5	theme	layered	163:169	arg1	structure					177:185	a unique porous layered chain structure	147:185	a unique porous layered chain structure	147:185	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	0	6	theme	Cu	97:98	arg1	removal					86:92	the removal	82:92	the removal of Cu(II), Pb(II), and Ni(II)	82:122	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	7	7	theme	adsorption	864:873	arg1	capacity					875:882	The maximum adsorption capacity	852:882	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II)	852:957	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	7	7	theme	adsorption	864:873	arg1	368.32					962:967	368.32	962:967	368.32	962:967	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	10	8	theme	loaded	1290:1295	arg1	Fe3O4-ATP/EDTA/CS					1297:1313	Cu(II) loaded Fe3O4-ATP/EDTA/CS	1283:1313	Cu(II) loaded Fe3O4-ATP/EDTA/CS	1283:1313	In addition, Cu(II) loaded Fe3O4-ATP/EDTA/CS also showed good catalytic degradation performance for bisphenol A.					
33895175	10	9	theme	catalytic	1332:1340	arg1	performance					1354:1364	good catalytic degradation performance	1327:1364	good catalytic degradation performance for bisphenol A	1327:1380	In addition, Cu(II) loaded Fe3O4-ATP/EDTA/CS also showed good catalytic degradation performance for bisphenol A.					
33895175	1	10	theme	chain	171:175	arg1	structure					177:185	a unique porous layered chain structure	147:185	a unique porous layered chain structure	147:185	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	4	11	from	beads	478:482	arg1	embedded					448:455	embedded	448:455	embedded	448:455	Then, Fe3O4-ATP nanoparticles were embedded in chitosan (CS) gel beads, and glutaraldehyde was used for cross-linking and curing.					
33895175	5	12	theme	ethylenediaminetetraacetic	551:576	arg1	EDTA					584:587	EDTA	584:587	EDTA	584:587	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	5	12	theme	ethylenediaminetetraacetic	551:576	arg1	acid					578:581	ethylenediaminetetraacetic acid	551:581	ethylenediaminetetraacetic acid (EDTA)	551:588	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	9	13	contain	has	1148:1150	arg1	Fe3O4-ATP/EDTA/CS					1130:1146	Fe3O4-ATP/EDTA/CS	1130:1146	Fe3O4-ATP/EDTA/CS	1130:1146	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	9	13	contain	has	1148:1150	arg2	capacity					1171:1178	a large adsorption capacity	1152:1178	a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation	1152:1267	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	9	14	theme	heavy	1184:1188	arg1	ions					1196:1199	heavy metal ions	1184:1199	heavy metal ions	1184:1199	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	11	15	theme	new	1405:1407	arg1	type					1409:1412	a new type	1403:1412	a new type of adsorbent with good adsorption performance	1403:1458	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	8	16	theme	good	1073:1076	arg1	capacity					1089:1096	good adsorption capacity	1073:1096	good adsorption capacity for Cu(II), Pb(II), and Ni(II)	1073:1127	After 5 times of repeated use, Fe3O4-ATP/EDTA/CS still showed good adsorption capacity for Cu(II), Pb(II), and Ni(II).					
33895175	9	17	theme	metal	1190:1194	arg1	ions					1196:1199	heavy metal ions	1184:1199	heavy metal ions	1184:1199	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	9	18	theme	wide	1204:1207	arg1	applicability					1212:1224	a wide pH applicability	1202:1224	a wide pH applicability	1202:1224	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	4	19	theme	gel	474:476	arg1	beads					478:482	chitosan (CS) gel beads	460:482	chitosan (CS) gel beads	460:482	Then, Fe3O4-ATP nanoparticles were embedded in chitosan (CS) gel beads, and glutaraldehyde was used for cross-linking and curing.					
33895175	10	20	theme	good	1327:1330	arg1	performance					1354:1364	good catalytic degradation performance	1327:1364	good catalytic degradation performance for bisphenol A	1327:1380	In addition, Cu(II) loaded Fe3O4-ATP/EDTA/CS also showed good catalytic degradation performance for bisphenol A.					
33895175	10	21	theme	Cu	1283:1284	arg1	Fe3O4-ATP/EDTA/CS					1297:1313	Cu(II) loaded Fe3O4-ATP/EDTA/CS	1283:1313	Cu(II) loaded Fe3O4-ATP/EDTA/CS	1283:1313	In addition, Cu(II) loaded Fe3O4-ATP/EDTA/CS also showed good catalytic degradation performance for bisphenol A.					
33895175	3	22	theme	magnetic	329:336	arg1	nanoparticles					344:356	magnetic Fe3O4 nanoparticles	329:356	magnetic Fe3O4 nanoparticles	329:356	First, it was combined with magnetic Fe3O4 nanoparticles to obtain Fe3O4-ATP that is easy to recycle and reuse.					
33895175	4	23	theme	chitosan	460:467	arg1	beads					478:482	chitosan (CS) gel beads	460:482	chitosan (CS) gel beads	460:482	Then, Fe3O4-ATP nanoparticles were embedded in chitosan (CS) gel beads, and glutaraldehyde was used for cross-linking and curing.					
33895175	5	24	theme	Fe3O4-ATP/CS	620:631	arg1	beads					637:641	Fe3O4-ATP/CS gel beads	620:641	Fe3O4-ATP/CS gel beads	620:641	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	3	25	theme	Fe3O4	338:342	arg1	nanoparticles					344:356	magnetic Fe3O4 nanoparticles	329:356	magnetic Fe3O4 nanoparticles	329:356	First, it was combined with magnetic Fe3O4 nanoparticles to obtain Fe3O4-ATP that is easy to recycle and reuse.					
33895175	0	26	theme	magnetic	29:36	arg1	chitosan					50:57	EDTA-modified magnetic attapulgite chitosan	15:57	EDTA-modified magnetic attapulgite chitosan gel bead adsorbent	15:76	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	9	27	theme	pH	1209:1210	arg1	applicability					1212:1224	a wide pH applicability	1202:1224	a wide pH applicability	1202:1224	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	8	28	theme	adsorption	1078:1087	arg1	capacity					1089:1096	good adsorption capacity	1073:1096	good adsorption capacity for Cu(II), Pb(II), and Ni(II)	1073:1127	After 5 times of repeated use, Fe3O4-ATP/EDTA/CS still showed good adsorption capacity for Cu(II), Pb(II), and Ni(II).					
33895175	0	29	theme	EDTA-modified	15:27	arg1	chitosan					50:57	EDTA-modified magnetic attapulgite chitosan	15:57	EDTA-modified magnetic attapulgite chitosan gel bead adsorbent	15:76	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	11	30	theme	wastewater	1513:1522	arg1	treatment					1500:1508	the treatment	1496:1508	the treatment of wastewater	1496:1522	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	4	31	used	used	508:511	arg2	glutaraldehyde					489:502	glutaraldehyde	489:502	glutaraldehyde	489:502	Then, Fe3O4-ATP nanoparticles were embedded in chitosan (CS) gel beads, and glutaraldehyde was used for cross-linking and curing.					
33895175	11	32	theme	adsorbent	1417:1425	arg1	performance					1448:1458	adsorbent with good adsorption performance	1417:1458	adsorbent with good adsorption performance	1417:1458	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	11	33	with	adsorbent	1417:1425	arg1	adsorption					1437:1446	good adsorption	1432:1446	good adsorption	1432:1446	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	5	34	theme	heavy	737:741	arg1	ions					749:752	heavy metal ions	737:752	heavy metal ions	737:752	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	0	35	theme	chitosan	50:57	arg1	Preparation					0:10	Preparation	0:10	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).	0:123	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	1	36	theme	excellent	197:205	arg1	decolorizer					228:238	an excellent environment-friendly decolorizer	194:238	an excellent environment-friendly decolorizer	194:238	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	9	37	theme	large	1154:1158	arg1	capacity					1171:1178	a large adsorption capacity	1152:1178	a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation	1152:1267	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	10	38	theme	bisphenol	1370:1378	arg1	A					1380:1380	bisphenol A	1370:1380	bisphenol A	1370:1380	In addition, Cu(II) loaded Fe3O4-ATP/EDTA/CS also showed good catalytic degradation performance for bisphenol A.					
33895175	5	39	theme	metal	743:747	arg1	ions					749:752	heavy metal ions	737:752	heavy metal ions	737:752	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	0	40	theme	attapulgite	38:48	arg1	chitosan					50:57	EDTA-modified magnetic attapulgite chitosan	15:57	EDTA-modified magnetic attapulgite chitosan gel bead adsorbent	15:76	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	1	41	theme	environment-friendly	207:226	arg1	decolorizer					228:238	an excellent environment-friendly decolorizer	194:238	an excellent environment-friendly decolorizer	194:238	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	0	42	theme	Pb	105:106	arg1	removal					86:92	the removal	82:92	the removal of Cu(II), Pb(II), and Ni(II)	82:122	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	5	43	theme	gel	633:635	arg1	beads					637:641	Fe3O4-ATP/CS gel beads	620:641	Fe3O4-ATP/CS gel beads	620:641	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	5	44	theme	adsorbent	717:725	arg1	capability					699:708	the capability	695:708	the capability of the adsorbent to adsorb heavy metal ions	695:752	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	9	45	theme	adsorption	1160:1169	arg1	capacity					1171:1178	a large adsorption capacity	1152:1178	a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation	1152:1267	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	8	46	theme	use	1037:1039	arg1	times					1019:1023	5 times	1017:1023	5 times of repeated use	1017:1039	After 5 times of repeated use, Fe3O4-ATP/EDTA/CS still showed good adsorption capacity for Cu(II), Pb(II), and Ni(II).					
33895175	4	47	theme	Fe3O4-ATP	419:427	arg1	nanoparticles					429:441	Fe3O4-ATP nanoparticles	419:441	Fe3O4-ATP nanoparticles	419:441	Then, Fe3O4-ATP nanoparticles were embedded in chitosan (CS) gel beads, and glutaraldehyde was used for cross-linking and curing.					
33895175	1	48	contain	has	143:145	arg2	structure					177:185	a unique porous layered chain structure	147:185	a unique porous layered chain structure	147:185	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	1	48	contain	has	143:145	arg1	Attapulgite					125:135	Attapulgite	125:135	Attapulgite (ATP)	125:141	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	1	48	contain	has	143:145	arg1	ATP					138:140	ATP	138:140	ATP	138:140	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
33895175	9	49	theme	good	1227:1230	arg1	reuse					1232:1236	good reuse	1227:1236	good reuse	1227:1236	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	8	50	theme	repeated	1028:1035	arg1	use					1037:1039	repeated use	1028:1039	repeated use	1028:1039	After 5 times of repeated use, Fe3O4-ATP/EDTA/CS still showed good adsorption capacity for Cu(II), Pb(II), and Ni(II).					
33895175	11	51	theme	performance	1448:1458	arg1	type					1409:1412	a new type	1403:1412	a new type of adsorbent with good adsorption performance	1403:1458	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	7	52	theme	Fe3O4-ATP/EDTA/CS	900:916	arg1	capacity					875:882	The maximum adsorption capacity	852:882	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II)	852:957	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	7	52	theme	Fe3O4-ATP/EDTA/CS	900:916	arg1	368.32					962:967	368.32	962:967	368.32	962:967	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	2	53	theme	high	254:257	arg1	viscosity					259:267	its high viscosity	250:267	its high viscosity	250:267	However, its high viscosity makes it difficult to separate.					
33895175	7	54	theme	220.31	982:987	arg1	g-1					992:994	220.31 mg g-1	982:994	220.31 mg g-1	982:994	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	7	55	theme	adsorbent	918:926	arg1	Fe3O4-ATP/EDTA/CS					900:916	the prepared Fe3O4-ATP/EDTA/CS	887:916	the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II)	887:957	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	11	56	theme	promising	1475:1483	arg1	method					1485:1490	a promising method	1473:1490	a promising method for the treatment of wastewater	1473:1522	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	6	57	with	sphere	802:807	arg1	surface					822:828	a rough surface	814:828	a rough surface	814:828	The prepared magnetic composite adsorbent is a sphere with a rough surface and porous structure.					
33895175	6	57	with	sphere	802:807	arg1	structure					841:849	porous structure	834:849	porous structure	834:849	The prepared magnetic composite adsorbent is a sphere with a rough surface and porous structure.					
33895175	11	58	theme	good	1432:1435	arg1	adsorption					1437:1446	good adsorption	1432:1446	good adsorption	1432:1446	This study prepared a new type of adsorbent with good adsorption performance and provided a promising method for the treatment of wastewater.					
33895175	5	59	theme	amidation	651:659	arg1	reaction					661:668	amidation reaction	651:668	amidation reaction	651:668	Lastly, ethylenediaminetetraacetic acid (EDTA) was successfully connected to Fe3O4-ATP/CS gel beads through amidation reaction, which further increased the capability of the adsorbent to adsorb heavy metal ions.					
33895175	7	60	theme	prepared	891:898	arg1	Fe3O4-ATP/EDTA/CS					900:916	the prepared Fe3O4-ATP/EDTA/CS	887:916	the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II)	887:957	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	9	61	theme	rapid	1243:1247	arg1	separation					1258:1267	rapid magnetic separation	1243:1267	rapid magnetic separation	1243:1267	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	0	62	theme	adsorbent	68:76	arg1	chitosan					50:57	EDTA-modified magnetic attapulgite chitosan	15:57	EDTA-modified magnetic attapulgite chitosan gel bead adsorbent	15:76	Preparation of EDTA-modified magnetic attapulgite chitosan gel bead adsorbent for the removal of Cu(II), Pb(II), and Ni(II).					
33895175	6	63	theme	rough	816:820	arg1	surface					822:828	a rough surface	814:828	a rough surface	814:828	The prepared magnetic composite adsorbent is a sphere with a rough surface and porous structure.					
33895175	7	64	theme	mg	989:990	arg1	g-1					992:994	220.31 mg g-1	982:994	220.31 mg g-1	982:994	The maximum adsorption capacity of the prepared Fe3O4-ATP/EDTA/CS adsorbent for Pb(II), Cu(II), and Ni(II) is 368.32, 267.94, and 220.31 mg g-1, respectively.					
33895175	9	65	theme	magnetic	1249:1256	arg1	separation					1258:1267	rapid magnetic separation	1243:1267	rapid magnetic separation	1243:1267	Fe3O4-ATP/EDTA/CS has a large adsorption capacity for heavy metal ions, a wide pH applicability, good reuse, and rapid magnetic separation.					
33895175	6	66	theme	porous	834:839	arg1	structure					841:849	porous structure	834:849	porous structure	834:849	The prepared magnetic composite adsorbent is a sphere with a rough surface and porous structure.					
33895175	1	67	theme	unique	149:154	arg1	structure					177:185	a unique porous layered chain structure	147:185	a unique porous layered chain structure	147:185	Attapulgite (ATP) has a unique porous layered chain structure and is an excellent environment-friendly decolorizer.					
32939740	7	0	theme	amaranth-based	1154:1167	arg1	beverage					1169:1176	amaranth-based beverage	1154:1176	amaranth-based beverage	1154:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	7	1	theme	enhanced	1061:1068	arg1	value					1079:1083	enhanced absolute value	1061:1083	enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage	1061:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	5	2	from	decrease	769:776	arg1	sensitivity					785:795	the sensitivity	781:795	the sensitivity to heat treatment of the proteins	781:829	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	1	3	theme	amaranth	123:130	arg1	proteins					132:139	amaranth proteins	123:139	amaranth proteins	123:139	The objective of this study was to formulate a beverage based on amaranth proteins, stable and nutritious.					
32939740	7	4	theme	beverage	1169:1176	arg1	stability					1141:1149	the high colloidal stability	1122:1149	the high colloidal stability of amaranth-based beverage	1122:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	7	5	theme	absolute	1070:1077	arg1	value					1079:1083	enhanced absolute value	1061:1083	enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage	1061:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	2	6	theme	separation	252:261	arg1	techniques					263:272	starch separation techniques	245:272	starch separation techniques	245:272	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	4	7	dep	3.42 ± 0.08	511:521	arg1	%					579:579	3 and 90.58 ± 0.01%	561:579	3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water	561:639	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	4	7	dep	3.42 ± 0.08	511:521	arg1	0.43 ± 0.01					548:558	0.43 ± 0.01	548:558	0.43 ± 0.01	548:558	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	4	7	dep	3.42 ± 0.08	511:521	arg1	1.9 ± 0.4					537:545	1.9 ± 0.4	537:545	1.9 ± 0.4	537:545	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	4	7	dep	3.42 ± 0.08	511:521	arg1	0.60 ± 0.06					524:534	0.60 ± 0.06	524:534	0.60 ± 0.06	524:534	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	2	8	theme	starch	245:250	arg1	techniques					263:272	starch separation techniques	245:272	starch separation techniques	245:272	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	7	9	theme	ζ-potential	1088:1098	arg1	value					1079:1083	enhanced absolute value	1061:1083	enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage	1061:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	5	10	theme	heat	800:803	arg1	treatment					805:813	heat treatment	800:813	heat treatment of the proteins	800:829	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	7	11	theme	high	1126:1129	arg1	stability					1141:1149	the high colloidal stability	1122:1149	the high colloidal stability of amaranth-based beverage	1122:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	7	12	theme	gums	1026:1029	arg1	addition					1014:1021	addition	1014:1021	addition of gums	1014:1029	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	7	12	theme	gums	1026:1029	arg1	treatment					1000:1008	Heat treatment	995:1008	Heat treatment	995:1008	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	5	13	theme	Thermal	657:663	arg1	treatment					665:673	Thermal treatment	657:673	Thermal treatment	657:673	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	4	14	dep	obtained	501:508	arg1	3.42 ± 0.08					511:521	3.42 ± 0.08	511:521	3.42 ± 0.08	511:521	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	0	15	theme	Beverage	30:37	arg1	Development					0:10	Development	0:10	Development of a High Protein Beverage	0:37	Development of a High Protein Beverage Based on Amaranth.					
32939740	1	16	theme	stable	142:147	arg1	proteins					132:139	amaranth proteins	123:139	amaranth proteins	123:139	The objective of this study was to formulate a beverage based on amaranth proteins, stable and nutritious.					
32939740	5	17	theme	proteins	722:729	arg1	aggregation					703:713	aggregation	703:713	aggregation	703:713	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	5	17	theme	proteins	722:729	arg1	denaturation					686:697	denaturation	686:697	denaturation	686:697	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	2	18	theme	techno-functional	278:294	arg1	properties					296:305	techno-functional properties	278:305	techno-functional properties of the amaranth proteins	278:330	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	1	19	theme	nutritious	153:162	arg1	proteins					132:139	amaranth proteins	123:139	amaranth proteins	123:139	The objective of this study was to formulate a beverage based on amaranth proteins, stable and nutritious.					
32939740	0	20	theme	High	17:20	arg1	Beverage					30:37	a High Protein Beverage	15:37	a High Protein Beverage	15:37	Development of a High Protein Beverage Based on Amaranth.					
32939740	6	21	theme	complexes	880:888	arg1	Formation					832:840	Formation	832:840	Formation of protein aggregates and gum-protein complexes	832:888	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	1	22	theme	study	80:84	arg1	objective					62:70	The objective	58:70	The objective of this study	58:84	The objective of this study was to formulate a beverage based on amaranth proteins, stable and nutritious.					
32939740	6	23	theme	gum-protein	868:878	arg1	complexes					880:888	gum-protein complexes	868:888	gum-protein complexes	868:888	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	5	24	dep	denaturation	686:697	arg1	the					682:684	the	682:684	the	682:684	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	6	25	theme	size	976:979	arg1	distribution					981:992	particle size distribution	967:992	particle size distribution	967:992	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	3	26	theme	protein	376:382	arg1	extract					384:390	the protein extract	372:390	the protein extract	372:390	Gums, gellan and xanthan were added to the protein extract and it was heat-treated at 80 °C during 20 min.					
32939740	6	27	theme	particle	967:974	arg1	distribution					981:992	particle size distribution	967:992	particle size distribution	967:992	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	5	28	theme	gums	754:757	arg1	addition					742:749	the addition	738:749	the addition of gums	738:757	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	0	29	theme	Protein	22:28	arg1	Beverage					30:37	a High Protein Beverage	15:37	a High Protein Beverage	15:37	Development of a High Protein Beverage Based on Amaranth.					
32939740	6	30	theme	aggregates	853:862	arg1	Formation					832:840	Formation	832:840	Formation of protein aggregates and gum-protein complexes	832:888	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	7	31	theme	Heat	995:998	arg1	treatment					1000:1008	Heat treatment	995:1008	Heat treatment	995:1008	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	7	32	theme	colloidal	1131:1139	arg1	stability					1141:1149	the high colloidal stability	1122:1149	the high colloidal stability of amaranth-based beverage	1122:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	4	33	theme	skim	481:484	arg1	milk					492:495	skim cow's milk	481:495	skim cow's milk	481:495	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	3	34	from	80 °C	419:423	arg1	heat-treated					403:414	heat-treated	403:414	heat-treated	403:414	Gums, gellan and xanthan were added to the protein extract and it was heat-treated at 80 °C during 20 min.					
32939740	2	35	theme	proteins	323:330	arg1	techniques					263:272	starch separation techniques	245:272	starch separation techniques	245:272	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	2	35	theme	proteins	323:330	arg1	properties					296:305	techno-functional properties	278:305	techno-functional properties of the amaranth proteins	278:330	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	2	36	theme	amaranth	314:321	arg1	proteins					323:330	the amaranth proteins	310:330	the amaranth proteins	310:330	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	2	37	theme	existing	220:227	arg1	knowledge					229:237	the existing knowledge	216:237	the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins	216:330	The process of obtaining the beverage was based on the existing knowledge about starch separation techniques and techno-functional properties of the amaranth proteins.					
32939740	5	38	theme	proteins	822:829	arg1	treatment					805:813	heat treatment	800:813	heat treatment of the proteins	800:829	Thermal treatment caused the denaturation and aggregation of the proteins, while the addition of gums induced a decrease in the sensitivity to heat treatment of the proteins.					
32939740	8	39	theme	lactose	1238:1244	arg1	intolerants					1246:1256	lactose intolerants	1238:1256	lactose intolerants	1238:1256	This beverage is suitable for vegans, celiac patients, and lactose intolerants.					
32939740	8	40	theme	celiac	1217:1222	arg1	patients					1224:1231	celiac patients	1217:1231	celiac patients	1217:1231	This beverage is suitable for vegans, celiac patients, and lactose intolerants.					
32939740	7	41	with	macrocomplexes	1041:1054	arg1	value					1079:1083	enhanced absolute value	1061:1083	enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage	1061:1176	Heat treatment and addition of gums generated macrocomplexes with enhanced absolute value of ζ-potential, which contributed to the high colloidal stability of amaranth-based beverage.					
32939740	6	42	theme	scanning	941:948	arg1	calorimetry					950:960	differential scanning calorimetry	928:960	differential scanning calorimetry	928:960	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	6	43	theme	differential	928:939	arg1	calorimetry					950:960	differential scanning calorimetry	928:960	differential scanning calorimetry	928:960	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
32939740	4	44	with	beverage	442:449	arg1	composition					458:468	a composition	456:468	a composition similar to skim cow's milk	456:495	A beverage with a composition similar to skim cow's milk was obtained (3.42 ± 0.08; 0.60 ± 0.06; 1.9 ± 0.4; 0.43 ± 0.01; 3 and 90.58 ± 0.01% for proteins, lipids, fiber, ashes, carbohydrates and water, respectively).					
32939740	6	45	theme	protein	845:851	arg1	aggregates					853:862	protein aggregates	845:862	protein aggregates	845:862	Formation of protein aggregates and gum-protein complexes was characterized by electrophoresis, differential scanning calorimetry, and particle size distribution.					
34741774	9	0	contain	had	1352:1354	arg1	groups					1345:1350	The F_Col and F groups	1329:1350	The F_Col and F groups	1329:1350	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	9	0	contain	had	1352:1354	arg2	reduction					1358:1366	a reduction	1356:1366	a reduction in calcium loss	1356:1382	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	8	1	theme	scanning	1229:1236	arg1	electron					1238:1245	scanning electron	1229:1245	scanning electron	1229:1245	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	11	2	theme	fluoridated	1540:1550	arg1	solutions					1573:1581	The fluoridated chitosan or collagen solutions	1536:1581	The fluoridated chitosan or collagen solutions	1536:1581	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	4	3	theme	phosphate	571:579	arg1	gel					596:598	acidulated phosphate fluoride (APF) gel	560:598	acidulated phosphate fluoride (APF) gel	560:598	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	0	4	theme	dentin	85:90	arg1	coverage					92:99	dentin coverage	85:99	dentin coverage	85:99	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	4	5	theme	chitosan	504:511	arg1	influence					491:499	the influence	487:499	the influence	487:499	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	6	6	theme	immersion	979:987	arg1	cycles					969:974	six cycles	965:974	six cycles	965:974	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	4	7	theme	acidulated	560:569	arg1	gel					596:598	acidulated phosphate fluoride (APF) gel	560:598	acidulated phosphate fluoride (APF) gel	560:598	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	0	8	theme	Chemical	102:109	arg1	analysis					129:136	Chemical and morphological analysis	102:136	Chemical and morphological analysis	102:136	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	4	9	from	influence	491:499	arg1	composition					608:618	composition	608:618	composition	608:618	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	4	9	from	influence	491:499	arg1	morphology					624:633	morphology	624:633	morphology	624:633	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	2	10	theme	dental	299:304	arg1	surfaces					306:313	dental surfaces	299:313	dental surfaces	299:313	The use of natural solutions to modify dental surfaces has become an area of research.					
34741774	11	11	theme	chitosan	1552:1559	arg1	solutions					1573:1581	The fluoridated chitosan or collagen solutions	1536:1581	The fluoridated chitosan or collagen solutions	1536:1581	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	11	12	from	erosion	1711:1717	arg1	protection					1695:1704	surface protection	1687:1704	surface protection from erosion	1687:1717	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	1	13	dep	prevention	143:152	arg1	The					139:141	The	139:141	The	139:141	The prevention and treatment of erosive tooth wear are becoming increasingly important due to its increasing prevalence.					
34741774	4	14	theme	fluoride	581:588	arg1	gel					596:598	acidulated phosphate fluoride (APF) gel	560:598	acidulated phosphate fluoride (APF) gel	560:598	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	3	15	theme	Organic	347:353	arg1	materials					355:363	Organic materials	347:363	Organic materials such as chitosan and hydrolyzed collagen	347:404	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	3	15	theme	Organic	347:353	arg1	option					425:430	a promising option	413:430	a promising option to treat dentin	413:446	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	3	15	theme	Organic	347:353	arg1	collagen					397:404	hydrolyzed collagen	386:404	hydrolyzed collagen	386:404	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	3	15	theme	Organic	347:353	arg1	chitosan					373:380	chitosan	373:380	chitosan	373:380	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	8	16	theme	Micro-energy	1169:1180	arg1	spectroscopy					1212:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	6	17	theme	Erosive	949:955	arg1	cycles					957:962	Erosive cycles	949:962	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr)	949:1052	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	6	18	dep	cycles	957:962	arg1	cycles					969:974	six cycles	965:974	six cycles	965:974	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	8	19	used	used	1252:1255	arg2	electron					1238:1245	scanning electron	1229:1245	scanning electron	1229:1245	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	8	19	used	used	1252:1255	arg2	spectroscopy					1212:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	9	20	theme	F_Col	1333:1337	arg1	groups					1345:1350	The F_Col and F groups	1329:1350	The F_Col and F groups	1329:1350	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	5	21	dep	control	754:760	arg1	AS					741:742	AS	741:742	AS	741:742	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	2	22	theme	solutions	279:287	arg1	use					264:266	The use	260:266	The use of natural solutions to modify dental surfaces	260:313	The use of natural solutions to modify dental surfaces has become an area of research.					
34741774	8	23	theme	chemical	1292:1299	arg1	composition					1301:1311	the dentin's inorganic chemical composition	1269:1311	the dentin's inorganic chemical composition	1269:1311	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	1	24	theme	wear	185:188	arg1	treatment					158:166	treatment	158:166	treatment	158:166	The prevention and treatment of erosive tooth wear are becoming increasingly important due to its increasing prevalence.					
34741774	1	24	theme	wear	185:188	arg1	prevention					143:152	prevention	143:152	prevention	143:152	The prevention and treatment of erosive tooth wear are becoming increasingly important due to its increasing prevalence.					
34741774	3	25	theme	hydrolyzed	386:395	arg1	collagen					397:404	hydrolyzed collagen	386:404	hydrolyzed collagen	386:404	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	10	26	theme	dentin	1495:1500	arg1	surface					1502:1508	the dentin surface	1491:1508	the dentin surface	1491:1508	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34741774	11	27	theme	novel	1630:1634	arg1	approach					1667:1674	a novel hybrid-fluoride-based material approach	1628:1674	a novel hybrid-fluoride-based material approach to provide surface protection from erosion	1628:1717	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	11	27	theme	novel	1630:1634	arg1	resistance					1603:1612	the dentin resistance	1592:1612	the dentin resistance to erosion	1592:1623	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	9	28	dep	respectively	1399:1410	arg1	p < .001					1413:1420	p < .001	1413:1420	p < .001	1413:1420	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	6	29	from	immersion	1028:1036	arg1	AS					1041:1042	AS	1041:1042	AS	1041:1042	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	4	30	theme	dentin	638:643	arg1	composition					608:618	composition	608:618	composition	608:618	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	4	30	theme	dentin	638:643	arg1	morphology					624:633	morphology	624:633	morphology	624:633	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	0	31	theme	chitosan	29:36	arg1	materials					57:65	chitosan and collagen-based materials	29:65	chitosan and collagen-based materials with fluoride for dentin coverage	29:99	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	8	32	theme	X-ray	1193:1197	arg1	spectroscopy					1212:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	5	33	dep	saliva	733:738	arg1	control					754:760	negative control	745:760	negative control	745:760	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	6	34	from	cycles	969:974	arg1	juice					999:1003	orange juice	992:1003	orange juice for 1 min	992:1013	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	5	35	theme	collagen	919:926	arg1	composition					928:938	fluoride/hydrolyzed collagen composition	899:938	fluoride/hydrolyzed collagen composition (F_Col)	899:946	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	35	theme	collagen	919:926	arg1	F_Col					941:945	F_Col	941:945	F_Col	941:945	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	6	36	from	immersion	979:987	arg1	juice					999:1003	orange juice	992:1003	orange juice for 1 min	992:1013	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	11	37	theme	material	1658:1665	arg1	approach					1667:1674	a novel hybrid-fluoride-based material approach	1628:1674	a novel hybrid-fluoride-based material approach to provide surface protection from erosion	1628:1717	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	11	37	theme	material	1658:1665	arg1	resistance					1603:1612	the dentin resistance	1592:1612	the dentin resistance to erosion	1592:1623	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	9	38	theme	calcium	1371:1377	arg1	loss					1379:1382	calcium loss	1371:1382	calcium loss	1371:1382	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	5	39	theme	APF	764:766	arg1	F					773:773	F	773:773	F	773:773	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	39	theme	APF	764:766	arg1	gel					768:770	APF gel	764:770	APF gel (F, positive control)	764:792	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	0	40	theme	collagen-based	42:55	arg1	materials					57:65	chitosan and collagen-based materials	29:65	chitosan and collagen-based materials with fluoride for dentin coverage	29:99	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	5	41	theme	hydrolyzed	820:829	arg1	Col					850:852	Col	850:852	Col	850:852	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	41	theme	hydrolyzed	820:829	arg1	solution					840:847	hydrolyzed collagen solution	820:847	hydrolyzed collagen solution (Col)	820:853	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	11	42	theme	hybrid-fluoride-based	1636:1656	arg1	approach					1667:1674	a novel hybrid-fluoride-based material approach	1628:1674	a novel hybrid-fluoride-based material approach to provide surface protection from erosion	1628:1717	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	11	42	theme	hybrid-fluoride-based	1636:1656	arg1	resistance					1603:1612	the dentin resistance	1592:1612	the dentin resistance to erosion	1592:1623	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	0	43	theme	morphological	115:127	arg1	analysis					129:136	Chemical and morphological analysis	102:136	Chemical and morphological analysis	102:136	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	5	44	theme	chitosan	795:802	arg1	Chi					814:816	Chi	814:816	Chi	814:816	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	44	theme	chitosan	795:802	arg1	solution					804:811	chitosan solution	795:811	chitosan solution (Chi)	795:817	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	10	45	theme	erosive	1520:1526	arg1	cycles					1528:1533	the erosive cycles	1516:1533	the erosive cycles	1516:1533	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34741774	3	46	theme	promising	415:423	arg1	materials					355:363	Organic materials	347:363	Organic materials such as chitosan and hydrolyzed collagen	347:404	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	3	46	theme	promising	415:423	arg1	option					425:430	a promising option	413:430	a promising option to treat dentin	413:446	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	3	46	theme	promising	415:423	arg1	collagen					397:404	hydrolyzed collagen	386:404	hydrolyzed collagen	386:404	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	3	46	theme	promising	415:423	arg1	chitosan					373:380	chitosan	373:380	chitosan	373:380	Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin.					
34741774	4	47	theme	hydrolyzed	516:525	arg1	collagen					527:534	hydrolyzed collagen	516:534	hydrolyzed collagen	516:534	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	4	48	dep	in	454:455	arg1	vitro					457:461	vitro	457:461	vitro	457:461	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	8	49	theme	fluorescence	1199:1210	arg1	spectroscopy					1212:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	4	50	dep	composition	608:618	arg1	the					604:606	the	604:606	the	604:606	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	9	51	from	reduction	1358:1366	arg1	loss					1379:1382	calcium loss	1371:1382	calcium loss	1371:1382	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	1	52	theme	increasing	237:246	arg1	prevalence					248:257	its increasing prevalence	233:257	its increasing prevalence	233:257	The prevention and treatment of erosive tooth wear are becoming increasingly important due to its increasing prevalence.					
34741774	5	53	theme	negative	745:752	arg1	control					754:760	negative control	745:760	negative control	745:760	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	9	54	theme	F	1343:1343	arg1	groups					1345:1350	The F_Col and F groups	1329:1350	The F_Col and F groups	1329:1350	The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001).					
34741774	4	55	theme	in	454:455	arg1	study					463:467	This in vitro study	449:467	This in vitro study	449:467	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	10	56	theme	covering	1457:1464	arg1	layer					1466:1470	a covering layer	1455:1470	a covering layer of agglomerates	1455:1486	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34741774	7	57	theme	size	1150:1153	arg1	distribution					1155:1166	particle size distribution	1141:1166	particle size distribution	1141:1166	The materials were characterized by their morphology, composition, and particle size distribution.					
34741774	11	58	theme	surface	1687:1693	arg1	protection					1695:1704	surface protection	1687:1704	surface protection from erosion	1687:1717	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	5	59	dep	F	773:773	arg1	control					785:791	positive control	776:791	positive control	776:791	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	2	60	theme	research	337:344	arg1	area					329:332	an area	326:332	an area of research	326:344	The use of natural solutions to modify dental surfaces has become an area of research.					
34741774	4	61	theme	collagen	527:534	arg1	influence					491:499	the influence	487:499	the influence	487:499	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	5	62	theme	Bovine	660:665	arg1	samples					674:680	Bovine dentin samples	660:680	Bovine dentin samples	660:680	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	2	63	theme	natural	271:277	arg1	solutions					279:287	natural solutions	271:287	natural solutions	271:287	The use of natural solutions to modify dental surfaces has become an area of research.					
34741774	8	64	theme	inorganic	1282:1290	arg1	composition					1301:1311	the dentin's inorganic chemical composition	1269:1311	the dentin's inorganic chemical composition	1269:1311	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	5	65	theme	collagen	831:838	arg1	Col					850:852	Col	850:852	Col	850:852	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	65	theme	collagen	831:838	arg1	solution					840:847	hydrolyzed collagen solution	820:847	hydrolyzed collagen solution (Col)	820:853	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	66	theme	dentin	667:672	arg1	samples					674:680	Bovine dentin samples	660:680	Bovine dentin samples	660:680	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	11	67	theme	collagen	1564:1571	arg1	solutions					1573:1581	The fluoridated chitosan or collagen solutions	1536:1581	The fluoridated chitosan or collagen solutions	1536:1581	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	5	68	theme	fluoride/chitosan	856:872	arg1	composition					874:884	fluoride/chitosan composition	856:884	fluoride/chitosan composition (F_Chi)	856:892	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	68	theme	fluoride/chitosan	856:872	arg1	F_Chi					887:891	F_Chi	887:891	F_Chi	887:891	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	7	69	theme	particle	1141:1148	arg1	distribution					1155:1166	particle size distribution	1141:1166	particle size distribution	1141:1166	The materials were characterized by their morphology, composition, and particle size distribution.					
34741774	4	70	theme	APF	591:593	arg1	gel					596:598	acidulated phosphate fluoride (APF) gel	560:598	acidulated phosphate fluoride (APF) gel	560:598	This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion.					
34741774	5	71	dep	prepared	687:694	arg1	n = 84					697:702	n = 84	697:702	n = 84	697:702	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	72	theme	positive	776:783	arg1	control					785:791	positive control	776:791	positive control	776:791	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	0	73	dep	induced	18:24	arg1	analysis					129:136	Chemical and morphological analysis	102:136	Chemical and morphological analysis	102:136	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	0	74	with	materials	57:65	arg1	fluoride					72:79	fluoride	72:79	fluoride	72:79	Biomineralization induced by chitosan and collagen-based materials with fluoride for dentin coverage: Chemical and morphological analysis.					
34741774	8	75	theme	dispersive	1182:1191	arg1	spectroscopy					1212:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy	1169:1223	Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology.					
34741774	11	76	theme	dentin	1596:1601	arg1	resistance					1603:1612	the dentin resistance	1592:1612	the dentin resistance to erosion	1592:1623	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	11	76	theme	dentin	1596:1601	arg1	approach					1667:1674	a novel hybrid-fluoride-based material approach	1628:1674	a novel hybrid-fluoride-based material approach to provide surface protection from erosion	1628:1717	The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion.					
34741774	5	77	theme	artificial	722:731	arg1	saliva					733:738	artificial saliva	722:738	artificial saliva (AS, negative control)	722:761	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	10	78	contain	had	1451:1453	arg2	layer					1466:1470	a covering layer	1455:1470	a covering layer of agglomerates	1455:1486	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34741774	10	78	contain	had	1451:1453	arg1	Both					1424:1427	Both	1424:1427	Both	1424:1427	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34741774	10	78	contain	had	1451:1453	arg1	groups					1438:1443	these groups	1432:1443	these groups	1432:1443	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34741774	5	79	theme	fluoride/hydrolyzed	899:917	arg1	composition					928:938	fluoride/hydrolyzed collagen composition	899:938	fluoride/hydrolyzed collagen composition (F_Col)	899:946	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	5	79	theme	fluoride/hydrolyzed	899:917	arg1	F_Col					941:945	F_Col	941:945	F_Col	941:945	Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col).					
34741774	6	80	from	juice	999:1003	arg1	cycles					969:974	six cycles	965:974	six cycles	965:974	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	6	81	theme	orange	992:997	arg1	juice					999:1003	orange juice	992:1003	orange juice for 1 min	992:1013	Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed.					
34741774	10	82	theme	agglomerates	1475:1486	arg1	layer					1466:1470	a covering layer	1455:1470	a covering layer of agglomerates	1455:1486	Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles.					
34246674	8	0	theme	amount	1197:1202	arg1	impact					1179:1184	The impact	1175:1184	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO	1175:1315	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	7	1	theme	antimicrobial	1021:1033	arg1	tests					1035:1039	The antimicrobial tests	1017:1039	The antimicrobial tests	1017:1039	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	1	2	theme	dye	174:176	arg1	properties					193:202	enhanced antibacterial and dye decolorization properties	147:202	enhanced antibacterial and dye decolorization properties for water treatment	147:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	4	3	theme	positive	611:618	arg1	bacteria					620:627	two Gram positive bacteria	602:627	two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus)	602:671	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	0	4	theme	adsorptive	89:98	arg1	films					100:104	adsorptive films	89:104	adsorptive films	89:104	New and efficient NiO/chitosan/polyvinyl alcohol nanocomposites as antibacterial and dye adsorptive films.					
34246674	1	5	theme	decolorization	178:191	arg1	properties					193:202	enhanced antibacterial and dye decolorization properties	147:202	enhanced antibacterial and dye decolorization properties for water treatment	147:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	4	6	theme	Gram	606:609	arg1	bacteria					620:627	two Gram positive bacteria	602:627	two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus)	602:671	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	4	7	dep	bacteria	695:702	arg1	coli					717:720	Escherichia coli	705:720	Escherichia coli	705:720	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	4	7	dep	bacteria	695:702	arg1	Typhimurium					737:747	Salmonella Typhimurium	726:747	Salmonella Typhimurium	726:747	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	2	8	theme	dual	399:402	arg1	functionality					404:416	a dual functionality	397:416	a dual functionality	397:416	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	8	theme	dual	399:402	arg1	removal					419:425	removal	419:425	removal of toxic dye and antibacterial properties	419:467	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	9	9	from	percentage	1448:1457	arg1	comparison					1462:1471	comparison	1462:1471	comparison with the unmodified PVA/chitosan film	1462:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	9	10	dep	increase	1420:1427	arg1	fold					1415:1418	fold	1415:1418	fold	1415:1418	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	9	11	theme	unmodified	1482:1491	arg1	film					1506:1509	the unmodified PVA/chitosan film	1478:1509	the unmodified PVA/chitosan film	1478:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	9	12	theme	removal	1440:1446	arg1	percentage					1448:1457	the dye removal percentage	1432:1457	the dye removal percentage in comparison with the unmodified PVA/chitosan film	1432:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	7	13	contain	have	1088:1091	arg1	films					1082:1086	the modified chitosan/PVA films	1056:1086	the modified chitosan/PVA films	1056:1086	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	7	13	contain	have	1088:1091	arg2	activity					1100:1107	higher activity	1093:1107	higher activity	1093:1107	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	2	14	theme	NiO	374:376	arg1	nanoparticles					378:390	NiO nanoparticles	374:390	NiO nanoparticles	374:390	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	5	15	theme	prepared	767:774	arg1	samples					776:782	The prepared samples	763:782	The prepared samples	763:782	The prepared samples were characterized by XRD, HRTEM, FESEM, ATR-FTIR, UV-Vis spectroscopy, and dielectric measurements.					
34246674	7	16	theme	tested	1147:1152	arg1	bacteria					1165:1172	all the tested pathogenic bacteria	1139:1172	all the tested pathogenic bacteria	1139:1172	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	8	17	theme	dose	1270:1273	arg1	impact					1179:1184	The impact	1175:1184	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO	1175:1315	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	2	18	theme	nanocomposite	312:324	arg1	films					326:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films	272:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	272:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	19	theme	toxic	430:434	arg1	dye					436:438	toxic dye	430:438	toxic dye	430:438	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	3	20	theme	Methyl	485:490	arg1	MO					500:501	MO	500:501	MO	500:501	Methyl orange (MO) was selected as a target pollutant.					
34246674	3	20	theme	Methyl	485:490	arg1	orange					492:497	Methyl orange	485:497	Methyl orange (MO)	485:502	Methyl orange (MO) was selected as a target pollutant.					
34246674	3	20	theme	Methyl	485:490	arg1	pollutant					529:537	a target pollutant	520:537	a target pollutant	520:537	Methyl orange (MO) was selected as a target pollutant.					
34246674	2	21	with	percentage	360:369	arg1	functionality					404:416	a dual functionality	397:416	a dual functionality	397:416	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	21	with	percentage	360:369	arg1	removal					419:425	removal	419:425	removal of toxic dye and antibacterial properties	419:467	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	7	22	theme	modified	1060:1067	arg1	films					1082:1086	the modified chitosan/PVA films	1056:1086	the modified chitosan/PVA films	1056:1086	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	7	23	theme	pathogenic	1154:1163	arg1	bacteria					1165:1172	all the tested pathogenic bacteria	1139:1172	all the tested pathogenic bacteria	1139:1172	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	9	24	from	increase	1420:1427	arg1	percentage					1448:1457	the dye removal percentage	1432:1457	the dye removal percentage in comparison with the unmodified PVA/chitosan film	1432:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	2	25	theme	alcohol	304:310	arg1	films					326:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films	272:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	272:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	26	theme	dye	436:438	arg1	functionality					404:416	a dual functionality	397:416	a dual functionality	397:416	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	26	theme	dye	436:438	arg1	removal					419:425	removal	419:425	removal of toxic dye and antibacterial properties	419:467	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	8	27	theme	NiO	1193:1195	arg1	%					1226:1226	0.5, 1.5, 3, and 5 wt%	1205:1226	0.5, 1.5, 3, and 5 wt%	1205:1226	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	8	27	theme	NiO	1193:1195	arg1	amount					1197:1202	the NiO amount	1189:1202	the NiO amount (0.5, 1.5, 3, and 5 wt%)	1189:1227	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	8	28	theme	MO	1314:1315	arg1	removal					1303:1309	the removal	1299:1309	the removal of MO	1299:1315	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	1	29	theme	water	208:212	arg1	treatment					214:222	water treatment	208:222	water treatment	208:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	0	30	theme	New	0:2	arg1	alcohol					41:47	New and efficient NiO/chitosan/polyvinyl alcohol	0:47	New and efficient NiO/chitosan/polyvinyl alcohol	0:47	New and efficient NiO/chitosan/polyvinyl alcohol nanocomposites as antibacterial and dye adsorptive films.					
34246674	4	31	theme	films	588:592	arg1	activity					572:579	the antimicrobial activity	554:579	the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium)	554:748	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	1	32	with	films	136:140	arg1	properties					193:202	enhanced antibacterial and dye decolorization properties	147:202	enhanced antibacterial and dye decolorization properties for water treatment	147:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	9	33	theme	PVA/chitosan	1493:1504	arg1	film					1506:1509	the unmodified PVA/chitosan film	1478:1509	the unmodified PVA/chitosan film	1478:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	8	34	from	impact	1179:1184	arg1	removal					1303:1309	the removal	1299:1309	the removal of MO	1299:1315	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	9	35	theme	dye	1436:1438	arg1	percentage					1448:1457	the dye removal percentage	1432:1457	the dye removal percentage in comparison with the unmodified PVA/chitosan film	1432:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	0	36	theme	NiO/chitosan/polyvinyl	18:39	arg1	alcohol					41:47	New and efficient NiO/chitosan/polyvinyl alcohol	0:47	New and efficient NiO/chitosan/polyvinyl alcohol	0:47	New and efficient NiO/chitosan/polyvinyl alcohol nanocomposites as antibacterial and dye adsorptive films.					
34246674	5	37	theme	ATR-FTIR	825:832	arg1	spectroscopy					842:853	ATR-FTIR, UV-Vis spectroscopy	825:853	spectroscopy	842:853	The prepared samples were characterized by XRD, HRTEM, FESEM, ATR-FTIR, UV-Vis spectroscopy, and dielectric measurements.					
34246674	2	38	theme	weight	353:358	arg1	percentage					360:369	different weight percentage	343:369	different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	343:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	9	39	theme	%	1390:1390	arg1	NiO					1392:1394	5 wt% NiO	1386:1394	5 wt% NiO	1386:1394	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	0	40	theme	efficient	8:16	arg1	alcohol					41:47	New and efficient NiO/chitosan/polyvinyl alcohol	0:47	New and efficient NiO/chitosan/polyvinyl alcohol	0:47	New and efficient NiO/chitosan/polyvinyl alcohol nanocomposites as antibacterial and dye adsorptive films.					
34246674	6	41	theme	unmodified	992:1001	arg1	chitosan/PVA					1003:1014	the unmodified chitosan/PVA	988:1014	the unmodified chitosan/PVA	988:1014	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	6	42	theme	morphological	889:901	arg1	examination					903:913	The morphological examination	885:913	The morphological examination	885:913	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	2	43	theme	different	343:351	arg1	percentage					360:369	different weight percentage	343:369	different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	343:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	8	44	dep	dose	1270:1273	arg1	40					1276:1277	40	1276:1277	40	1276:1277	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	8	44	dep	dose	1270:1273	arg1	80					1280:1281	80	1280:1281	80	1280:1281	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	8	44	dep	dose	1270:1273	arg1	100 mg					1288:1293	100 mg	1288:1293	100 mg	1288:1293	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	2	45	contain	containing	332:341	arg1	films					326:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films	272:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	272:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	45	contain	containing	332:341	arg2	percentage					360:369	different weight percentage	343:369	different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	343:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	4	46	dep	bacteria	620:627	arg1	aureus					645:650	Staphylococcus aureus	630:650	Staphylococcus aureus	630:650	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	4	46	dep	bacteria	620:627	arg1	cereus					665:670	Bacillus cereus	656:670	Bacillus cereus	656:670	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	0	47	dep	antibacterial	67:79	arg1	films					100:104	adsorptive films	89:104	adsorptive films	89:104	New and efficient NiO/chitosan/polyvinyl alcohol nanocomposites as antibacterial and dye adsorptive films.					
34246674	9	48	theme	NiO	1392:1394	arg1	incorporation					1369:1381	the incorporation	1365:1381	the incorporation of 5 wt% NiO	1365:1394	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	2	49	theme	properties	458:467	arg1	functionality					404:416	a dual functionality	397:416	a dual functionality	397:416	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	2	49	theme	properties	458:467	arg1	removal					419:425	removal	419:425	removal of toxic dye and antibacterial properties	419:467	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	4	50	theme	negative	686:693	arg1	bacteria					695:702	two Gram negative bacteria	677:702	two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium)	677:748	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	4	51	theme	antimicrobial	558:570	arg1	activity					572:579	the antimicrobial activity	554:579	the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium)	554:748	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	7	52	theme	pure	1114:1117	arg1	chitosan/PVA					1119:1130	pure chitosan/PVA	1114:1130	pure chitosan/PVA toward all the tested pathogenic bacteria	1114:1172	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	8	53	theme	adsorbent	1260:1268	arg1	dose					1270:1273	adsorbent dose	1260:1273	adsorbent dose (40, 80, and 100 mg)	1260:1294	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	5	54	theme	dielectric	860:869	arg1	measurements					871:882	dielectric measurements	860:882	dielectric measurements	860:882	The prepared samples were characterized by XRD, HRTEM, FESEM, ATR-FTIR, UV-Vis spectroscopy, and dielectric measurements.					
34246674	1	55	theme	great	240:244	arg1	attention					246:254	a great attention	238:254	a great attention	238:254	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	9	56	theme	5 wt	1386:1389	arg1	NiO					1392:1394	5 wt% NiO	1386:1394	5 wt% NiO	1386:1394	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	7	57	theme	higher	1093:1098	arg1	activity					1100:1107	higher activity	1093:1107	higher activity	1093:1107	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	7	58	theme	chitosan/PVA	1069:1080	arg1	films					1082:1086	the modified chitosan/PVA films	1056:1086	the modified chitosan/PVA films	1056:1086	The antimicrobial tests indicated that the modified chitosan/PVA films have higher activity than pure chitosan/PVA toward all the tested pathogenic bacteria.					
34246674	1	59	theme	composite	126:134	arg1	films					136:140	composite films	126:140	composite films with enhanced antibacterial and dye decolorization properties for water treatment	126:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	6	60	theme	nanocomposite	931:943	arg1	film					945:948	the nanocomposite film	927:948	the nanocomposite film	927:948	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	1	61	theme	films	136:140	arg1	development					111:121	The development	107:121	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment	107:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	6	62	theme	porous	959:964	arg1	structure					966:974	more porous structure	954:974	more porous structure	954:974	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	2	63	theme	oxide/chitosan/polyvinyl	279:302	arg1	films					326:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films	272:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	272:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	6	64	contain	has	950:952	arg1	film					945:948	the nanocomposite film	927:948	the nanocomposite film	927:948	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	6	64	contain	has	950:952	arg2	structure					966:974	more porous structure	954:974	more porous structure	954:974	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	2	65	theme	nanoparticles	378:390	arg1	percentage					360:369	different weight percentage	343:369	different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	343:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	9	66	dep	fold	1415:1418	arg1	2					1413:1413	2	1413:1413	2	1413:1413	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	8	67	theme	contact	1230:1236	arg1	0-150 min					1244:1252	0-150 min	1244:1252	0-150 min	1244:1252	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	8	67	theme	contact	1230:1236	arg1	time					1238:1241	contact time	1230:1241	contact time (0-150 min)	1230:1253	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	2	68	theme	nickel	272:277	arg1	films					326:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films	272:330	nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties,	272:468	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	1	69	theme	enhanced	147:154	arg1	properties					193:202	enhanced antibacterial and dye decolorization properties	147:202	enhanced antibacterial and dye decolorization properties for water treatment	147:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	9	70	theme	Dye	1330:1332	arg1	results					1345:1351	Dye adsorption results	1330:1351	Dye adsorption results	1330:1351	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	9	71	with	comparison	1462:1471	arg1	film					1506:1509	the unmodified PVA/chitosan film	1478:1509	the unmodified PVA/chitosan film	1478:1509	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	2	72	theme	antibacterial	444:456	arg1	properties					458:467	antibacterial properties	444:467	antibacterial properties	444:467	In this study, nickel oxide/chitosan/polyvinyl alcohol nanocomposite films containing different weight percentage of NiO nanoparticles with a dual functionality, removal of toxic dye and antibacterial properties, were prepared.					
34246674	1	73	theme	antibacterial	156:168	arg1	properties					193:202	enhanced antibacterial and dye decolorization properties	147:202	enhanced antibacterial and dye decolorization properties for water treatment	147:222	The development of composite films with enhanced antibacterial and dye decolorization properties for water treatment has attracted a great attention.					
34246674	4	74	theme	Gram	681:684	arg1	bacteria					695:702	two Gram negative bacteria	677:702	two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium)	677:748	Additionally, the antimicrobial activity of the films against two Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram negative bacteria (Escherichia coli and Salmonella Typhimurium) was studied.					
34246674	9	75	theme	adsorption	1334:1343	arg1	results					1345:1351	Dye adsorption results	1330:1351	Dye adsorption results	1330:1351	Dye adsorption results proved that the incorporation of 5 wt% NiO led to more than 2 fold increase in the dye removal percentage in comparison with the unmodified PVA/chitosan film.					
34246674	6	76	theme	more	954:957	arg1	structure					966:974	more porous structure	954:974	more porous structure	954:974	The morphological examination proved that the nanocomposite film has more porous structure compared to the unmodified chitosan/PVA.					
34246674	5	77	theme	UV-Vis	835:840	arg1	spectroscopy					842:853	ATR-FTIR, UV-Vis spectroscopy	825:853	spectroscopy	842:853	The prepared samples were characterized by XRD, HRTEM, FESEM, ATR-FTIR, UV-Vis spectroscopy, and dielectric measurements.					
34246674	8	78	theme	time	1238:1241	arg1	impact					1179:1184	The impact	1175:1184	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO	1175:1315	The impact of the NiO amount (0.5, 1.5, 3, and 5 wt%), contact time (0-150 min), and adsorbent dose (40, 80, and 100 mg) on the removal of MO was studied.					
34246674	3	79	theme	target	522:527	arg1	orange					492:497	Methyl orange	485:497	Methyl orange (MO)	485:502	Methyl orange (MO) was selected as a target pollutant.					
34246674	3	79	theme	target	522:527	arg1	pollutant					529:537	a target pollutant	520:537	a target pollutant	520:537	Methyl orange (MO) was selected as a target pollutant.					
33650274	0	0	theme	Flow	95:98	arg1	sorbents					100:107	Sepharose Fast Flow sorbents	80:107	Sepharose Fast Flow sorbents	80:107	Isothermal modelling of protein adsorption to thermo-responsive polymer grafted Sepharose Fast Flow sorbents.					
33650274	2	1	theme	bovine	864:869	arg1	protein					855:861	the homologous protein	840:861	the homologous protein	840:861	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	1	theme	bovine	864:869	arg1	holo-transferrin					871:886	bovine holo-transferrin	864:886	bovine holo-transferrin	864:886	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	0	2	theme	Fast	90:93	arg1	sorbents					100:107	Sepharose Fast Flow sorbents	80:107	Sepharose Fast Flow sorbents	80:107	Isothermal modelling of protein adsorption to thermo-responsive polymer grafted Sepharose Fast Flow sorbents.					
33650274	1	3	theme	different	447:455	arg1	compositions					467:478	different copolymer compositions	447:478	different copolymer compositions	447:478	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	4	theme	-based	372:377	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	5	theme	copolymer	457:465	arg1	compositions					467:478	different copolymer compositions	447:478	different copolymer compositions	447:478	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	6	theme	protein	855:861	arg1	binding					829:835	the binding	825:835	the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents	825:944	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	7	theme	copolymer-grafted	919:935	arg1	sorbents					937:944	the same thermo-responsive copolymer-grafted sorbents	892:944	the same thermo-responsive copolymer-grafted sorbents	892:944	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	8	theme	bovine	621:626	arg1	holo-lactoferrin					628:643	bovine holo-lactoferrin	621:643	bovine holo-lactoferrin	621:643	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	9	theme	compositions	467:478	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	10	with	sorbents	435:442	arg1	holo-lactoferrin					534:549	bovine holo-lactoferrin	527:549	bovine holo-lactoferrin	527:549	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	10	with	sorbents	435:442	arg1	holo-transferrin					562:577	bovine holo-transferrin	555:577	bovine holo-transferrin at 20 and 50°C	555:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	10	with	sorbents	435:442	arg1	proteins					510:517	two structurally related proteins	485:517	two structurally related proteins	485:517	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	11	theme	thermo-responsive	901:917	arg1	sorbents					937:944	the same thermo-responsive copolymer-grafted sorbents	892:944	the same thermo-responsive copolymer-grafted sorbents	892:944	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	12	dep	Freundlich	683:692	arg1	isotherms					705:713	isotherms	705:713	isotherms	705:713	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	12	dep	Freundlich	683:692	arg1	the					679:681	the	679:681	the	679:681	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	13	theme	feed	732:735	arg1	concentrations					737:750	the protein feed concentrations	720:750	the protein feed concentrations	720:750	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	14	dep	Freundlich	1015:1024	arg1	isotherms					1037:1045	isotherms	1037:1045	isotherms with coefficients of determination value over 0.90	1037:1096	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	15	theme	protein	724:730	arg1	concentrations					737:750	the protein feed concentrations	720:750	the protein feed concentrations	720:750	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	0	16	theme	Isothermal	0:9	arg1	modelling					11:19	Isothermal modelling	0:19	Isothermal modelling of protein adsorption to thermo-responsive polymer	0:70	Isothermal modelling of protein adsorption to thermo-responsive polymer grafted Sepharose Fast Flow sorbents.					
33650274	1	17	theme	related	502:508	arg1	holo-lactoferrin					534:549	bovine holo-lactoferrin	527:549	bovine holo-lactoferrin	527:549	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	17	theme	related	502:508	arg1	holo-transferrin					562:577	bovine holo-transferrin	555:577	bovine holo-transferrin at 20 and 50°C	555:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	17	theme	related	502:508	arg1	proteins					510:517	two structurally related proteins	485:517	two structurally related proteins	485:517	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	18	theme	determination	1068:1080	arg1	value					1082:1086	determination value	1068:1086	determination value over 0.90	1068:1096	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	0	19	theme	protein	24:30	arg1	adsorption					32:41	protein adsorption	24:41	protein adsorption	24:41	Isothermal modelling of protein adsorption to thermo-responsive polymer grafted Sepharose Fast Flow sorbents.					
33650274	2	20	theme	experimental	964:975	arg1	data					977:980	the experimental data	960:980	the experimental data	960:980	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	21	theme	1-40 mg/mL	773:782	arg1	range					764:768	the range	760:768	the range of 1-40 mg/mL	760:782	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	22	used	utilized	264:271	arg2	models					150:155	five adsorption isotherm models	125:155	five adsorption isotherm models	125:155	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	22	used	utilized	264:271	arg2	Langmuir					171:178	the Langmuir	167:178	the Langmuir	167:178	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	23	theme	thermo-responsive	379:395	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	24	from	20	792:793	arg1	range					764:768	the range	760:768	the range of 1-40 mg/mL	760:782	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	25	theme	Similar	805:811	arg1	analysis					813:820	Similar analysis	805:820	Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents	805:944	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	0	26	theme	adsorption	32:41	arg1	modelling					11:19	Isothermal modelling	0:19	Isothermal modelling of protein adsorption to thermo-responsive polymer	0:70	Isothermal modelling of protein adsorption to thermo-responsive polymer grafted Sepharose Fast Flow sorbents.					
33650274	1	27	theme	copolymer-grafted	397:413	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	28	with	isotherms	1037:1045	arg1	coefficients					1052:1063	coefficients	1052:1063	coefficients of determination value over 0.90	1052:1096	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	29	theme	experimental	297:308	arg1	data					321:324	the experimental adsorption data	293:324	the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	293:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	30	theme	Sepharose	415:423	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	31	from	50°C.	799:803	arg1	range					764:768	the range	760:768	the range of 1-40 mg/mL	760:782	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	0	32	theme	thermo-responsive	46:62	arg1	polymer					64:70	thermo-responsive polymer	46:70	thermo-responsive polymer	46:70	Isothermal modelling of protein adsorption to thermo-responsive polymer grafted Sepharose Fast Flow sorbents.					
33650274	1	33	theme	adsorption	310:319	arg1	data					321:324	the experimental adsorption data	293:324	the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	293:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	34	theme	value	1082:1086	arg1	coefficients					1052:1063	coefficients	1052:1063	coefficients of determination value over 0.90	1052:1096	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	35	theme	Fast	425:428	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	36	theme	bovine	527:532	arg1	holo-lactoferrin					534:549	bovine holo-lactoferrin	527:549	bovine holo-lactoferrin	527:549	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	36	theme	bovine	527:532	arg1	proteins					510:517	two structurally related proteins	485:517	two structurally related proteins	485:517	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	37	theme	homologous	844:853	arg1	protein					855:861	the homologous protein	840:861	the homologous protein	840:861	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	37	theme	homologous	844:853	arg1	holo-transferrin					871:886	bovine holo-transferrin	864:886	bovine holo-transferrin	864:886	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	38	theme	data	321:324	arg1	analysis					281:288	the analysis	277:288	the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	277:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	39	theme	experimental	599:610	arg1	data					612:615	The experimental data	595:615	The experimental data for bovine holo-lactoferrin	595:643	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	40	theme	Flow	430:433	arg1	sorbents					435:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents	345:442	poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	345:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	41	from	50°C	589:592	arg1	holo-lactoferrin					534:549	bovine holo-lactoferrin	527:549	bovine holo-lactoferrin	527:549	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	41	from	50°C	589:592	arg1	holo-transferrin					562:577	bovine holo-transferrin	555:577	bovine holo-transferrin at 20 and 50°C	555:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	41	from	50°C	589:592	arg1	proteins					510:517	two structurally related proteins	485:517	two structurally related proteins	485:517	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	42	theme	same	896:899	arg1	sorbents					937:944	the same thermo-responsive copolymer-grafted sorbents	892:944	the same thermo-responsive copolymer-grafted sorbents	892:944	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	43	theme	sorbents	435:442	arg1	classes					334:340	six classes	330:340	six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C	330:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	44	theme	adsorption	130:139	arg1	models					150:155	five adsorption isotherm models	125:155	five adsorption isotherm models	125:155	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	44	theme	adsorption	130:139	arg1	Langmuir					171:178	the Langmuir	167:178	the Langmuir	167:178	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	45	from	20	582:583	arg1	holo-lactoferrin					534:549	bovine holo-lactoferrin	527:549	bovine holo-lactoferrin	527:549	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	45	from	20	582:583	arg1	holo-transferrin					562:577	bovine holo-transferrin	555:577	bovine holo-transferrin at 20 and 50°C	555:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	45	from	20	582:583	arg1	proteins					510:517	two structurally related proteins	485:517	two structurally related proteins	485:517	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	46	theme	bovine	555:560	arg1	holo-transferrin					562:577	bovine holo-transferrin	555:577	bovine holo-transferrin at 20 and 50°C	555:592	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	46	theme	bovine	555:560	arg1	proteins					510:517	two structurally related proteins	485:517	two structurally related proteins	485:517	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	47	theme	isotherm	141:148	arg1	models					150:155	five adsorption isotherm models	125:155	five adsorption isotherm models	125:155	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	1	47	theme	isotherm	141:148	arg1	Langmuir					171:178	the Langmuir	167:178	the Langmuir	167:178	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
33650274	2	48	theme	binding	829:835	arg1	analysis					813:820	Similar analysis	805:820	Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents	805:944	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	2	49	dep	50°C.	799:803	arg1	revealed					946:953	revealed	946:953	revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90	946:1096	The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo-transferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.					
33650274	1	50	theme	Brunauer-Emmett-Teller	225:246	arg1	isotherms					248:256	Brunauer-Emmett-Teller isotherms	225:256	Brunauer-Emmett-Teller isotherms	225:256	In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holo-transferrin at 20 and 50°C.					
32535914	5	0	theme	heating	689:695	arg1	stability					697:705	the heating stability	685:705	the heating stability	685:705	Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment.					
32535914	8	1	theme	starch-AP	1106:1114	arg1	mixture					1116:1122	the starch-AP mixture	1102:1122	the starch-AP mixture	1102:1122	These results indicated that UHP treatment inhibited the retrogradation of the starch-AP mixture.					
32535914	8	2	theme	UHP	1056:1058	arg1	treatment					1060:1068	UHP treatment	1056:1068	UHP treatment	1056:1068	These results indicated that UHP treatment inhibited the retrogradation of the starch-AP mixture.					
32535914	0	3	theme	rice	77:80	arg1	properties					63:72	structural and physicochemical properties	32:72	structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols	32:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	9	4	theme	pronounced	1225:1234	arg1	network					1236:1242	more pronounced network	1220:1242	more pronounced network	1220:1242	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	7	5	theme	infrared	868:875	arg1	spectroscopy					877:888	Fourier-transform infrared spectroscopy	850:888	Fourier-transform infrared spectroscopy	850:888	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	9	6	theme	mixtures	1182:1189	arg1	microstructures					1145:1159	the microstructures	1141:1159	the microstructures of CRS-AP and CCS-AP mixtures	1141:1189	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	0	7	theme	starch	91:96	arg1	properties					63:72	structural and physicochemical properties	32:72	structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols	32:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	0	8	from	Effect	0:5	arg1	properties					63:72	structural and physicochemical properties	32:72	structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols	32:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	3	9	theme	common	475:480	arg1	CCS					495:497	CCS	495:497	CCS	495:497	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	9	theme	common	475:480	arg1	starch					487:492	common corn starch	475:492	common corn starch (CCS)-AP mixtures	475:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	1	10	theme	food	260:263	arg1	texture					265:271	food texture	260:271	food texture	260:271	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	0	11	theme	corn	86:89	arg1	starch					91:96	corn starch	86:96	corn starch	86:96	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	6	12	theme	UHP	835:837	arg1	treatment					839:847	UHP treatment	835:847	UHP treatment	835:847	X-ray diffraction indicated that the relative crystallinity of the mixtures was unaffected by UHP treatment.					
32535914	7	13	theme	new	905:907	arg1	peaks					920:924	no new absorption peaks	902:924	no new absorption peaks	902:924	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	2	14	theme	UHP	278:280	arg1	treatments					282:291	UHP treatments	278:291	UHP treatments	278:291	How UHP treatments influence starch-polyphenol complexes has not yet been reported.					
32535914	4	15	theme	UHP	530:532	arg1	treatment					534:542	UHP treatment	530:542	UHP treatment	530:542	RESULTS Overall, UHP treatment decreased the particle size of the CRS-AP and CCS-AP composites.					
32535914	7	16	theme	absorption	909:918	arg1	peaks					920:924	no new absorption peaks	902:924	no new absorption peaks	902:924	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	3	17	theme	starch	487:492	arg1	mixtures					503:510	common corn starch (CCS)-AP mixtures	475:510	common corn starch (CCS)-AP mixtures	475:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	18	theme	common	424:429	arg1	CRS					444:446	CRS	444:446	CRS	444:446	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	18	theme	common	424:429	arg1	starch					436:441	common rice starch	424:441	common rice starch (CRS)-apple polyphenol (AP)	424:469	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	5	19	theme	UHP	726:728	arg1	treatment					730:738	UHP treatment	726:738	UHP treatment	726:738	Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment.					
32535914	3	20	from	effects	379:385	arg1	structure					411:419	the structure	407:419	the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures	407:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	10	21	theme	treatments	1459:1468	arg1	use					1448:1450	the use	1444:1450	the use of UHP treatments for food production	1444:1488	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	0	22	with	complexes	101:109	arg1	polyphenols					122:132	apple polyphenols	116:132	apple polyphenols	116:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	3	23	theme	rice	431:434	arg1	CRS					444:446	CRS	444:446	CRS	444:446	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	23	theme	rice	431:434	arg1	starch					436:441	common rice starch	424:441	common rice starch (CRS)-apple polyphenol (AP)	424:469	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	24	theme	corn	482:485	arg1	CCS					495:497	CCS	495:497	CCS	495:497	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	24	theme	corn	482:485	arg1	starch					487:492	common corn starch	475:492	common corn starch (CCS)-AP mixtures	475:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	10	25	theme	UHP	1455:1457	arg1	treatments					1459:1468	UHP treatments	1455:1468	UHP treatments	1455:1468	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	9	26	theme	CRS-AP	1164:1169	arg1	microstructures					1145:1159	the microstructures	1141:1159	the microstructures of CRS-AP and CCS-AP mixtures	1141:1189	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	10	27	theme	UHP	1399:1401	arg1	treatment					1403:1411	UHP treatment	1399:1411	UHP treatment	1399:1411	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	6	28	theme	relative	778:785	arg1	crystallinity					787:799	the relative crystallinity	774:799	the relative crystallinity of the mixtures	774:815	X-ray diffraction indicated that the relative crystallinity of the mixtures was unaffected by UHP treatment.					
32535914	0	29	theme	pressure	20:27	arg1	Effect					0:5	Effect	0:5	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.	0:133	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	0	30	theme	apple	116:120	arg1	polyphenols					122:132	apple polyphenols	116:132	apple polyphenols	116:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	9	31	theme	CCS-AP	1175:1180	arg1	mixtures					1182:1189	CCS-AP mixtures	1175:1189	CCS-AP mixtures	1175:1189	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	2	32	theme	starch-polyphenol	303:319	arg1	complexes					321:329	starch-polyphenol complexes	303:329	starch-polyphenol complexes	303:329	How UHP treatments influence starch-polyphenol complexes has not yet been reported.					
32535914	1	33	theme	non-thermal	184:194	arg1	technology					207:216	a non-thermal processing technology	182:216	a non-thermal processing technology	182:216	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	1	33	theme	non-thermal	184:194	arg1	treatment					171:179	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	0	34	theme	ultrahigh	10:18	arg1	pressure					20:27	ultrahigh pressure	10:27	ultrahigh pressure	10:27	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	11	35	theme	Chemical	1509:1516	arg1	Industry					1518:1525	Chemical Industry	1509:1525	Chemical Industry	1509:1525	© 2020 Society of Chemical Industry.					
32535914	6	36	theme	mixtures	808:815	arg1	crystallinity					787:799	the relative crystallinity	774:799	the relative crystallinity of the mixtures	774:815	X-ray diffraction indicated that the relative crystallinity of the mixtures was unaffected by UHP treatment.					
32535914	3	37	theme	mixtures	503:510	arg1	structure					411:419	the structure	407:419	the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures	407:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	7	38	located	observed	931:938	arg2	peaks					920:924	no new absorption peaks	902:924	no new absorption peaks	902:924	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	7	38	located	observed	931:938	arg1	spectra					956:962	the infrared spectra	943:962	the infrared spectra	943:962	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	1	39	theme	processing	196:205	arg1	technology					207:216	a non-thermal processing technology	182:216	a non-thermal processing technology	182:216	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	1	39	theme	processing	196:205	arg1	treatment					171:179	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	0	40	theme	structural	32:41	arg1	properties					63:72	structural and physicochemical properties	32:72	structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols	32:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	5	41	theme	CRS-AP	643:648	arg1	mixtures					661:668	the CRS-AP and CCS-AP mixtures	639:668	mixtures	661:668	Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment.					
32535914	0	42	from	rice	77:80	arg1	complexes					101:109	complexes	101:109	complexes with apple polyphenols	101:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	3	43	theme	starch	436:441	arg1	AP					467:468	AP	467:468	AP	467:468	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	43	theme	starch	436:441	arg1	polyphenol					455:464	common rice starch (CRS)-apple polyphenol	424:464	common rice starch (CRS)-apple polyphenol (AP)	424:469	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	4	44	theme	composites	597:606	arg1	size					567:570	the particle size	554:570	the particle size of the CRS-AP and CCS-AP composites	554:606	RESULTS Overall, UHP treatment decreased the particle size of the CRS-AP and CCS-AP composites.					
32535914	10	45	theme	food	1474:1477	arg1	production					1479:1488	food production	1474:1488	food production	1474:1488	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	5	46	theme	mixtures	661:668	arg1	values					629:634	the ΔH values	622:634	the ΔH values of the CRS-AP and CCS-AP mixtures	622:668	Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment.					
32535914	4	47	theme	CRS-AP	579:584	arg1	composites					597:606	the CRS-AP and CCS-AP composites	575:606	the CRS-AP and CCS-AP composites	575:606	RESULTS Overall, UHP treatment decreased the particle size of the CRS-AP and CCS-AP composites.					
32535914	0	48	theme	physicochemical	47:61	arg1	properties					63:72	structural and physicochemical properties	32:72	structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols	32:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	5	49	theme	CCS-AP	654:659	arg1	mixtures					661:668	the CRS-AP and CCS-AP mixtures	639:668	mixtures	661:668	Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment.					
32535914	7	50	theme	starch-AP	982:990	arg1	order					973:977	the order	969:977	the order of starch-AP	969:990	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	0	51	from	properties	63:72	arg1	complexes					101:109	complexes	101:109	complexes with apple polyphenols	101:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	10	52	dep	CONCLUSIONS	1276:1286	arg1	provide					1302:1308	provide	1302:1308	provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production	1302:1488	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	3	53	theme	treatment	394:402	arg1	effects					379:385	the effects	375:385	the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures	375:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	10	54	theme	mixtures	1380:1387	arg1	properties					1356:1365	the properties	1352:1365	the properties of starch-AP mixtures	1352:1387	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	7	55	theme	UHP	1012:1014	arg1	treatment					1016:1024	UHP treatment	1012:1024	UHP treatment	1012:1024	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	0	56	from	complexes	101:109	arg1	properties					63:72	structural and physicochemical properties	32:72	structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols	32:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	10	57	theme	theoretical	1312:1322	arg1	basis					1324:1328	a theoretical basis	1310:1328	a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production	1310:1488	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	9	58	theme	increased	1198:1206	arg1	folding					1208:1214	increased folding	1198:1214	increased folding	1198:1214	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	7	59	theme	Fourier-transform	850:866	arg1	spectroscopy					877:888	Fourier-transform infrared spectroscopy	850:888	Fourier-transform infrared spectroscopy	850:888	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	10	60	theme	starch-AP	1370:1378	arg1	mixtures					1380:1387	starch-AP mixtures	1370:1387	starch-AP mixtures	1370:1387	CONCLUSIONS These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production.					
32535914	5	61	theme	ΔH	626:627	arg1	values					629:634	the ΔH values	622:634	the ΔH values of the CRS-AP and CCS-AP mixtures	622:668	Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment.					
32535914	6	62	theme	X-ray	741:745	arg1	diffraction					747:757	X-ray diffraction	741:757	X-ray diffraction	741:757	X-ray diffraction indicated that the relative crystallinity of the mixtures was unaffected by UHP treatment.					
32535914	3	63	theme	-apple	448:453	arg1	AP					467:468	AP	467:468	AP	467:468	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	63	theme	-apple	448:453	arg1	polyphenol					455:464	common rice starch (CRS)-apple polyphenol	424:464	common rice starch (CRS)-apple polyphenol (AP)	424:469	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	3	64	theme	-AP	499:501	arg1	mixtures					503:510	common corn starch (CCS)-AP mixtures	475:510	common corn starch (CCS)-AP mixtures	475:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	8	65	theme	mixture	1116:1122	arg1	retrogradation					1084:1097	the retrogradation	1080:1097	the retrogradation of the starch-AP mixture	1080:1122	These results indicated that UHP treatment inhibited the retrogradation of the starch-AP mixture.					
32535914	0	66	from	starch	91:96	arg1	complexes					101:109	complexes	101:109	complexes with apple polyphenols	101:132	Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.					
32535914	3	67	theme	polyphenol	455:464	arg1	structure					411:419	the structure	407:419	the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures	407:510	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	9	68	theme	microstructures	1145:1159	arg1	analyses					1129:1136	Our analyses	1125:1136	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures	1125:1189	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	9	69	dep	folding	1208:1214	arg1	structures					1244:1253	structures	1244:1253	structures	1244:1253	Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.					
32535914	7	70	theme	infrared	947:954	arg1	spectra					956:962	the infrared spectra	943:962	the infrared spectra	943:962	Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment.					
32535914	1	71	theme	BACKGROUND	135:144	arg1	Ultrahigh-pressure					146:163	BACKGROUND Ultrahigh-pressure	135:163	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	1	71	theme	BACKGROUND	135:144	arg1	UHP					166:168	UHP	166:168	UHP	166:168	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	4	72	theme	CCS-AP	590:595	arg1	composites					597:606	the CRS-AP and CCS-AP composites	575:606	the CRS-AP and CCS-AP composites	575:606	RESULTS Overall, UHP treatment decreased the particle size of the CRS-AP and CCS-AP composites.					
32535914	1	73	theme	bactericidal	228:239	arg1	effect					241:246	a bactericidal effect	226:246	a bactericidal effect	226:246	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	3	74	theme	UHP	390:392	arg1	treatment					394:402	UHP treatment	390:402	UHP treatment	390:402	Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.					
32535914	1	75	theme	Ultrahigh-pressure	146:163	arg1	technology					207:216	a non-thermal processing technology	182:216	a non-thermal processing technology	182:216	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	1	75	theme	Ultrahigh-pressure	146:163	arg1	treatment					171:179	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment	135:179	BACKGROUND Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture.					
32535914	4	76	theme	particle	558:565	arg1	size					567:570	the particle size	554:570	the particle size of the CRS-AP and CCS-AP composites	554:606	RESULTS Overall, UHP treatment decreased the particle size of the CRS-AP and CCS-AP composites.					
32086941	15	0	theme	shear-thinning	2768:2781	arg1	behavior					2811:2818	shear-thinning and significant thixotropic behavior	2768:2818	shear-thinning and significant thixotropic behavior	2768:2818	The starch pastes are found to exhibit shear-thinning and significant thixotropic behavior.					
32086941	10	1	theme	yield	1999:2003	arg1	values					2012:2017	The yield stress values	1995:2017	The yield stress values	1995:2017	The yield stress values are found to be in good agreement when comparing various testing methods.					
32086941	3	2	theme	pressure	654:661	arg1	cells					663:667	traditional pressure cells	642:667	traditional pressure cells	642:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	2	3	theme	ingredients	350:360	arg1	boiling					330:336	the boiling	326:336	the boiling of volatile ingredients	326:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	8	4	theme	rheological	1623:1633	arg1	properties					1635:1644	the rheological properties	1619:1644	the rheological properties of the final starch paste	1619:1670	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	12	5	theme	yield	2390:2394	arg1	stress					2396:2401	no yield stress	2387:2401	no yield stress	2387:2401	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	9	6	theme	flow	1815:1818	arg1	ramps					1820:1824	flow ramps	1815:1824	flow ramps	1815:1824	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	8	7	theme	high	1574:1577	arg1	temperature					1579:1589	high temperature	1574:1589	high temperature	1574:1589	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	6	8	theme	shear	1220:1224	arg1	rate					1226:1229	a fixed shear rate	1212:1229	a fixed shear rate	1212:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	4	9	theme	sample	831:836	arg1	relationships					810:822	the structure-property relationships	787:822	the structure-property relationships of the sample	787:836	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	8	10	theme	final	1653:1657	arg1	paste					1666:1670	the final starch paste	1649:1670	the final starch paste	1649:1670	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	1	11	theme	shear	164:168	arg1	stresses					170:177	high shear stresses	159:177	high shear stresses	159:177	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	12	12	theme	ϕ - ϕc	2355:2360	arg1	m					2364:2364	(ϕ - ϕc ) m	2354:2364	(ϕ - ϕc ) m	2354:2364	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	3	13	theme	torque	560:565	arg1	sensitivity					567:577	torque sensitivity	560:577	torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells	560:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	8	14	theme	paste	1666:1670	arg1	properties					1635:1644	the rheological properties	1619:1644	the rheological properties of the final starch paste	1619:1670	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	8	14	theme	paste	1666:1670	arg1	process					1607:1613	the gelation process	1594:1613	the gelation process	1594:1613	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	7	15	theme	starch	1288:1293	arg1	slurry					1295:1300	an initial flowable starch slurry	1268:1300	an initial flowable starch slurry to a paste	1268:1311	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	6	16	theme	fractions	1130:1138	arg1	gelatinization					1059:1072	In situ gelatinization	1051:1072	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1051:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	10	17	theme	good	2038:2041	arg1	agreement					2043:2051	good agreement	2038:2051	good agreement	2038:2051	The yield stress values are found to be in good agreement when comparing various testing methods.					
32086941	14	18	theme	Herschel-Bulkley	2668:2683	arg1	model					2685:2689	The Herschel-Bulkley model	2664:2689	The Herschel-Bulkley model	2664:2689	The Herschel-Bulkley model is found to fit the flow curves well.					
32086941	3	19	theme	bearings	490:497	arg1	free					471:474	free	471:474	free	471:474	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	4	20	theme	industrial	902:911	arg1	processing					913:922	industrial processing	902:922	industrial processing	902:922	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	12	21	theme	weight	2335:2340	arg1	fraction					2342:2349	starch particle weight fraction	2319:2349	starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04	2319:2382	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	2	22	theme	water-based	220:230	arg1	formulations					232:243	water-based formulations	220:243	water-based formulations above 100°C	220:255	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	10	23	theme	various	2068:2074	arg1	methods					2084:2090	various testing methods	2068:2090	various testing methods	2068:2090	The yield stress values are found to be in good agreement when comparing various testing methods.					
32086941	5	24	theme	commercial	1013:1022	arg1	starch					1024:1029	a commercial starch	1011:1029	a commercial starch dispersed in water	1011:1048	This cell is employed to investigate the pasting properties of a commercial starch dispersed in water.					
32086941	2	25	theme	behavior	207:214	arg1	measurements					184:195	The measurements	180:195	The measurements of sample behavior for water-based formulations above 100°C	180:255	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	1	26	theme	extreme	105:111	arg1	conditions					124:133	extreme processing conditions	105:133	extreme processing conditions of high temperature and high shear stresses	105:177	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	8	27	theme	Typical	1393:1399	arg1	parameters					1401:1410	Typical parameters	1393:1410	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity	1393:1536	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	3	28	from	improvement	545:555	arg1	sensitivity					567:577	torque sensitivity	560:577	torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells	560:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	3	29	theme	10	606:607	arg1	μN.m					609:612	oscillatory and 10 μN.m	590:612	μN.m	609:612	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	13	30	theme	breakdown	2597:2605	arg1	viscosities					2607:2617	breakdown viscosities	2597:2617	breakdown viscosities	2597:2617	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	2	31	from	changes	289:295	arg1	composition					309:319	material composition	300:319	material composition from the boiling of volatile ingredients	300:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	2	31	from	changes	289:295	arg1	boiling					330:336	the boiling	326:336	the boiling of volatile ingredients	326:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	7	32	theme	flowable	1279:1286	arg1	slurry					1295:1300	an initial flowable starch slurry	1268:1300	an initial flowable starch slurry to a paste	1268:1311	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	15	33	theme	starch	2733:2738	arg1	pastes					2740:2745	The starch pastes	2729:2745	The starch pastes	2729:2745	The starch pastes are found to exhibit shear-thinning and significant thixotropic behavior.					
32086941	13	34	theme	yield	2450:2454	arg1	stress					2456:2461	yield stress	2450:2461	yield stress	2450:2461	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	3	35	theme	oscillatory	590:600	arg1	μN.m					609:612	oscillatory and 10 μN.m	590:612	μN.m	609:612	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	1	36	theme	high	138:141	arg1	temperature					143:153	high temperature	138:153	high temperature	138:153	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	3	37	dep	high-sensitivity	383:398	arg1	pressurized					401:411	pressurized	401:411	pressurized	401:411	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	11	38	theme	yield	2248:2252	arg1	stress					2254:2259	any measurable yield stress	2233:2259	any measurable yield stress	2233:2259	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	4	39	theme	volatile	733:740	arg1	components					742:751	the volatile components	729:751	the volatile components	729:751	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	9	40	theme	paste	1714:1718	arg1	stresses					1692:1699	yield stresses	1686:1699	yield stresses of the final paste, measured at 120°C,	1686:1738	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	0	41	from	temperatures	39:50	arg1	Rheology					0:7	Rheology	0:7	Rheology of starch dispersions at high temperatures.	0:51	Rheology of starch dispersions at high temperatures.					
32086941	9	42	theme	shear	1931:1935	arg1	studies					1913:1919	studies	1913:1919	studies of steady shear	1913:1935	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	42	theme	shear	1931:1935	arg1	behavior					1964:1971	nonlinear viscoelastic behavior	1941:1971	nonlinear viscoelastic behavior of the starch pastes	1941:1992	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	11	43	theme	Yield	2093:2097	arg1	stresses					2099:2106	Yield stresses	2093:2106	Yield stresses	2093:2106	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	7	44	from	slurry	1295:1300	arg1	transition					1251:1260	A phase transition	1243:1260	A phase transition	1243:1260	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	6	45	theme	In	1051:1052	arg1	gelatinization					1059:1072	In situ gelatinization	1051:1072	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1051:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	3	46	theme	high-sensitivity	383:398	arg1	bar					442:444	up to 5 bar	434:444	up to 5 bar	434:444	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	3	46	theme	high-sensitivity	383:398	arg1	cell					428:431	a high-sensitivity, pressurized starch pasting cell	381:431	a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells	381:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	9	47	theme	nonlinear	1941:1949	arg1	behavior					1964:1971	nonlinear viscoelastic behavior	1941:1971	nonlinear viscoelastic behavior of the starch pastes	1941:1992	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	13	48	theme	yield	2565:2569	arg1	stress					2571:2576	yield stress	2565:2576	yield stress	2565:2576	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	1	49	theme	many	75:78	arg1	products					85:92	many food products	75:92	many food products	75:92	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	11	50	dep	range	2108:2112	arg1	with					2163:2166	with	2163:2166	with	2163:2166	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	0	51	theme	starch	12:17	arg1	dispersions					19:29	starch dispersions	12:29	starch dispersions	12:29	Rheology of starch dispersions at high temperatures.					
32086941	3	52	theme	pasting	420:426	arg1	bar					442:444	up to 5 bar	434:444	up to 5 bar	434:444	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	3	52	theme	pasting	420:426	arg1	cell					428:431	a high-sensitivity, pressurized starch pasting cell	381:431	a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells	381:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	8	53	theme	peak	1501:1504	arg1	temperature					1506:1516	peak temperature	1501:1516	peak temperature	1501:1516	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	11	54	from	Pa	2131:2132	arg1	range					2108:2112	range	2108:2112	range	2108:2112	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	4	55	theme	structure-property	791:808	arg1	relationships					810:822	the structure-property relationships	787:822	the structure-property relationships of the sample	787:836	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	3	56	from	μN.m	582:585	arg1	μN.m					609:612	oscillatory and 10 μN.m	590:612	μN.m	609:612	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	3	57	dep	sensitivity	567:577	arg1	μN.m					582:585	1 μN.m	580:585	1 μN.m in oscillatory and 10 μN.m in shear flows	580:627	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	11	58	dep	6.5	2127:2129	arg1	to					2124:2125	to	2124:2125	to	2124:2125	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	9	59	theme	pastes	1987:1992	arg1	studies					1913:1919	studies	1913:1919	studies of steady shear	1913:1935	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	59	theme	pastes	1987:1992	arg1	behavior					1964:1971	nonlinear viscoelastic behavior	1941:1971	nonlinear viscoelastic behavior of the starch pastes	1941:1992	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	3	60	theme	free	471:474	arg1	design					464:469	a design	462:469	a design free of mechanical bearings and seals	462:507	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	3	61	dep	5	440:440	arg1	to					437:438	to	437:438	to	437:438	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	6	62	theme	starch	1107:1112	arg1	ϕ					1141:1141	ϕ	1141:1141	ϕ	1141:1141	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	6	62	theme	starch	1107:1112	arg1	fractions					1130:1138	varying starch particle weight fractions	1099:1138	varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1099:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	11	63	theme	starch	2191:2196	arg1	0.05					2168:2171	0.05	2168:2171	0.05	2168:2171	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	11	63	theme	starch	2191:2196	arg1	fraction					2205:2212	the minimum starch weight fraction	2179:2212	the minimum starch weight fraction for which there is any measurable yield stress	2179:2259	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	8	64	theme	peak	1523:1526	arg1	viscosity					1528:1536	peak viscosity	1523:1536	peak viscosity	1523:1536	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	3	65	theme	traditional	642:652	arg1	cells					663:667	traditional pressure cells	642:667	traditional pressure cells	642:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	8	66	theme	temperature	1579:1589	arg1	impact					1564:1569	the impact	1560:1569	the impact of high temperature on the gelation process and the rheological properties of the final starch paste	1560:1670	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	9	67	theme	traditional	1775:1785	arg1	ramps					1820:1824	flow ramps	1815:1824	flow ramps	1815:1824	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	67	theme	traditional	1775:1785	arg1	shear					1839:1843	oscillatory shear	1827:1843	oscillatory shear	1827:1843	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	67	theme	traditional	1775:1785	arg1	methods					1799:1805	traditional rheological methods	1775:1805	traditional rheological methods such as flow ramps, oscillatory shear, and stress growth	1775:1862	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	67	theme	traditional	1775:1785	arg1	growth					1857:1862	stress growth	1850:1862	stress growth	1850:1862	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	6	68	theme	starch	1077:1082	arg1	dispersions					1084:1094	starch dispersions	1077:1094	starch dispersions	1077:1094	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	10	69	theme	stress	2005:2010	arg1	values					2012:2017	The yield stress values	1995:2017	The yield stress values	1995:2017	The yield stress values are found to be in good agreement when comparing various testing methods.					
32086941	3	70	theme	mechanical	479:488	arg1	bearings					490:497	mechanical bearings	479:497	mechanical bearings	479:497	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	2	71	from	boiling	330:336	arg1	changes					289:295	changes	289:295	changes in material composition from the boiling of volatile ingredients	289:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	2	71	from	boiling	330:336	arg1	composition					309:319	material composition	300:319	material composition from the boiling of volatile ingredients	300:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	3	72	from	μN.m	609:612	arg1	flows					623:627	shear flows	617:627	shear flows	617:627	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	14	73	theme	flow	2711:2714	arg1	curves					2716:2721	the flow curves	2707:2721	the flow curves	2707:2721	The Herschel-Bulkley model is found to fit the flow curves well.					
32086941	13	74	theme	analytical	2520:2529	arg1	method					2531:2536	the analytical method	2516:2536	the analytical method used	2516:2541	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	7	75	theme	magnitude	1382:1390	arg1	orders					1372:1377	several orders	1364:1377	several orders of magnitude	1364:1390	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	7	76	theme	several	1364:1370	arg1	orders					1372:1377	several orders	1364:1377	several orders of magnitude	1364:1390	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	2	77	theme	volatile	341:348	arg1	ingredients					350:360	volatile ingredients	341:360	volatile ingredients	341:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	6	78	theme	fixed	1214:1218	arg1	rate					1226:1229	a fixed shear rate	1212:1229	a fixed shear rate	1212:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	1	79	theme	high	159:162	arg1	stresses					170:177	high shear stresses	159:177	high shear stresses	159:177	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	9	80	theme	varying	1757:1763	arg1	ϕ					1765:1765	varying ϕ	1757:1765	varying ϕ	1757:1765	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	81	theme	oscillatory	1827:1837	arg1	shear					1839:1843	oscillatory shear	1827:1843	oscillatory shear	1827:1843	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	13	82	theme	1.15-1.4	2494:2501	arg1	range					2485:2489	the range	2481:2489	the range of 1.15-1.4 depending on the analytical method used	2481:2541	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	13	82	theme	1.15-1.4	2494:2501	arg1	definition					2551:2560	the definition	2547:2560	the definition of yield stress	2547:2576	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	1	83	theme	stresses	170:177	arg1	conditions					124:133	extreme processing conditions	105:133	extreme processing conditions of high temperature and high shear stresses	105:177	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	12	84	theme	particle	2326:2333	arg1	fraction					2342:2349	starch particle weight fraction	2319:2349	starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04	2319:2382	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	3	85	theme	order-of-magnitude	526:543	arg1	improvement					545:555	an order-of-magnitude improvement	523:555	an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells	523:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	8	86	theme	starch	1659:1664	arg1	paste					1666:1670	the final starch paste	1649:1670	the final starch paste	1649:1670	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	6	87	theme	weight	1123:1128	arg1	ϕ					1141:1141	ϕ	1141:1141	ϕ	1141:1141	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	6	87	theme	weight	1123:1128	arg1	fractions					1130:1138	varying starch particle weight fractions	1099:1138	varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1099:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	9	88	theme	stress	1850:1855	arg1	growth					1857:1862	stress growth	1850:1862	stress growth	1850:1862	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	6	89	theme	elevated	1187:1194	arg1	pressure					1196:1203	elevated pressure	1187:1203	elevated pressure	1187:1203	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	10	90	theme	testing	2076:2082	arg1	methods					2084:2090	various testing methods	2068:2090	various testing methods	2068:2090	The yield stress values are found to be in good agreement when comparing various testing methods.					
32086941	4	91	theme	pressurized	672:682	arg1	atmosphere					684:693	A pressurized atmosphere	670:693	A pressurized atmosphere in the cell	670:705	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	8	92	from	impact	1564:1569	arg1	properties					1635:1644	the rheological properties	1619:1644	the rheological properties of the final starch paste	1619:1670	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	8	92	from	impact	1564:1569	arg1	process					1607:1613	the gelation process	1594:1613	the gelation process	1594:1613	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	5	93	theme	starch	1024:1029	arg1	properties					997:1006	the pasting properties	985:1006	the pasting properties of a commercial starch dispersed in water	985:1048	This cell is employed to investigate the pasting properties of a commercial starch dispersed in water.					
32086941	1	94	theme	processing	113:122	arg1	conditions					124:133	extreme processing conditions	105:133	extreme processing conditions of high temperature and high shear stresses	105:177	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	2	95	theme	material	300:307	arg1	composition					309:319	material composition	300:319	material composition from the boiling of volatile ingredients	300:360	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	12	96	theme	starch	2319:2324	arg1	fraction					2342:2349	starch particle weight fraction	2319:2349	starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04	2319:2382	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	4	97	dep	150°C	880:884	arg1	to					877:878	to	877:878	to	877:878	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	9	98	theme	yield	1686:1690	arg1	stresses					1692:1699	yield stresses	1686:1699	yield stresses of the final paste, measured at 120°C,	1686:1738	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	8	99	theme	viscosity	1432:1440	arg1	evolution					1442:1450	the viscosity evolution	1428:1450	the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity	1428:1536	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	6	100	theme	high	1159:1162	arg1	120°C					1177:1181	120°C	1177:1181	120°C	1177:1181	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	6	100	theme	high	1159:1162	arg1	temperature					1164:1174	a high temperature	1157:1174	a high temperature (120°C)	1157:1182	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	9	101	theme	cell	1904:1907	arg1	capabilities					1883:1894	the capabilities	1879:1894	the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes	1879:1992	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	1	102	theme	temperature	143:153	arg1	conditions					124:133	extreme processing conditions	105:133	extreme processing conditions of high temperature and high shear stresses	105:177	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	11	103	theme	measurable	2237:2246	arg1	stress					2254:2259	any measurable yield stress	2233:2259	any measurable yield stress	2233:2259	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	12	104	dep	stress	2272:2277	arg1	both					2303:2306	both	2303:2306	both	2303:2306	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	9	105	theme	final	1708:1712	arg1	paste					1714:1718	the final paste	1704:1718	the final paste	1704:1718	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	106	theme	steady	1924:1929	arg1	shear					1931:1935	steady shear	1924:1935	steady shear	1924:1935	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	1	107	theme	food	60:63	arg1	industry					65:72	the food industry	56:72	the food industry	56:72	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	8	108	theme	gelation	1598:1605	arg1	process					1607:1613	the gelation process	1594:1613	the gelation process	1594:1613	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	4	109	theme	storage	928:934	arg1	conditions					936:945	storage conditions	928:945	storage conditions	928:945	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	8	110	theme	onset	1482:1486	arg1	temperature					1488:1498	onset temperature	1482:1498	onset temperature	1482:1498	Typical parameters associated with the viscosity evolution during gelatinization such as onset temperature, peak temperature, and peak viscosity are analyzed to probe the impact of high temperature on the gelation process and the rheological properties of the final starch paste.					
32086941	4	111	theme	components	742:751	arg1	boiling					718:724	boiling	718:724	boiling of the volatile components	718:751	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	9	112	theme	viscoelastic	1951:1962	arg1	behavior					1964:1971	nonlinear viscoelastic behavior	1941:1971	nonlinear viscoelastic behavior of the starch pastes	1941:1992	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	11	113	theme	minimum	2183:2189	arg1	0.05					2168:2171	0.05	2168:2171	0.05	2168:2171	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	11	113	theme	minimum	2183:2189	arg1	fraction					2205:2212	the minimum starch weight fraction	2179:2212	the minimum starch weight fraction for which there is any measurable yield stress	2179:2259	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	0	114	theme	dispersions	19:29	arg1	Rheology					0:7	Rheology	0:7	Rheology of starch dispersions at high temperatures.	0:51	Rheology of starch dispersions at high temperatures.					
32086941	13	115	theme	stress	2571:2576	arg1	range					2485:2489	the range	2481:2489	the range of 1.15-1.4 depending on the analytical method used	2481:2541	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	13	115	theme	stress	2571:2576	arg1	definition					2551:2560	the definition	2547:2560	the definition of yield stress	2547:2576	The exponent, m, for yield stress is found to be in the range of 1.15-1.4 depending on the analytical method used and the definition of yield stress while for peak and breakdown viscosities it is noted to be 1.6 and 1.1, respectively.					
32086941	1	116	theme	food	80:83	arg1	products					85:92	many food products	75:92	many food products	75:92	In the food industry, many food products experience extreme processing conditions of high temperature and high shear stresses.					
32086941	7	117	theme	phase	1245:1249	arg1	transition					1251:1260	A phase transition	1243:1260	A phase transition	1243:1260	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	3	118	theme	shear	617:621	arg1	flows					623:627	shear flows	617:627	shear flows	617:627	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	6	119	dep	In	1051:1052	arg1	situ					1054:1057	situ	1054:1057	situ	1054:1057	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	15	120	theme	significant	2787:2797	arg1	behavior					2811:2818	shear-thinning and significant thixotropic behavior	2768:2818	shear-thinning and significant thixotropic behavior	2768:2818	The starch pastes are found to exhibit shear-thinning and significant thixotropic behavior.					
32086941	0	121	theme	high	34:37	arg1	temperatures					39:50	high temperatures	34:50	high temperatures	34:50	Rheology of starch dispersions at high temperatures.					
32086941	5	122	theme	pasting	989:995	arg1	properties					997:1006	the pasting properties	985:1006	the pasting properties of a commercial starch dispersed in water	985:1048	This cell is employed to investigate the pasting properties of a commercial starch dispersed in water.					
32086941	9	123	theme	rheological	1787:1797	arg1	ramps					1820:1824	flow ramps	1815:1824	flow ramps	1815:1824	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	123	theme	rheological	1787:1797	arg1	shear					1839:1843	oscillatory shear	1827:1843	oscillatory shear	1827:1843	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	123	theme	rheological	1787:1797	arg1	methods					1799:1805	traditional rheological methods	1775:1805	traditional rheological methods such as flow ramps, oscillatory shear, and stress growth	1775:1862	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	123	theme	rheological	1787:1797	arg1	growth					1857:1862	stress growth	1850:1862	stress growth	1850:1862	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	9	124	theme	starch	1980:1985	arg1	pastes					1987:1992	the starch pastes	1976:1992	the starch pastes	1976:1992	Furthermore, yield stresses of the final paste, measured at 120°C, are examined for varying ϕ through traditional rheological methods such as flow ramps, oscillatory shear, and stress growth, demonstrating the capabilities of this cell for studies of steady shear and nonlinear viscoelastic behavior of the starch pastes.					
32086941	6	125	theme	particle	1114:1121	arg1	ϕ					1141:1141	ϕ	1141:1141	ϕ	1141:1141	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	6	125	theme	particle	1114:1121	arg1	fractions					1130:1138	varying starch particle weight fractions	1099:1138	varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1099:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	3	126	theme	starch	413:418	arg1	bar					442:444	up to 5 bar	434:444	up to 5 bar	434:444	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	3	126	theme	starch	413:418	arg1	cell					428:431	a high-sensitivity, pressurized starch pasting cell	381:431	a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells	381:667	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32086941	12	127	theme	paste	2287:2291	arg1	viscosity					2293:2301	the paste viscosity	2283:2301	the paste viscosity	2283:2301	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	2	128	theme	sample	200:205	arg1	behavior					207:214	sample behavior	200:214	sample behavior	200:214	The measurements of sample behavior for water-based formulations above 100°C is extremely challenging due to changes in material composition from the boiling of volatile ingredients.					
32086941	4	129	theme	relationships	810:822	arg1	characterization					767:782	the characterization	763:782	the characterization of the structure-property relationships of the sample	763:836	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	6	130	theme	varying	1099:1105	arg1	ϕ					1141:1141	ϕ	1141:1141	ϕ	1141:1141	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	6	130	theme	varying	1099:1105	arg1	fractions					1130:1138	varying starch particle weight fractions	1099:1138	varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1099:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	11	131	theme	weight	2198:2203	arg1	0.05					2168:2171	0.05	2168:2171	0.05	2168:2171	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	11	131	theme	weight	2198:2203	arg1	fraction					2205:2212	the minimum starch weight fraction	2179:2212	the minimum starch weight fraction for which there is any measurable yield stress	2179:2259	Yield stresses range from 0.25 to 6.5 Pa for ϕ between 0.05 and 0.15, with 0.05 being the minimum starch weight fraction for which there is any measurable yield stress.					
32086941	15	132	theme	thixotropic	2799:2809	arg1	behavior					2811:2818	shear-thinning and significant thixotropic behavior	2768:2818	shear-thinning and significant thixotropic behavior	2768:2818	The starch pastes are found to exhibit shear-thinning and significant thixotropic behavior.					
32086941	4	133	from	atmosphere	684:693	arg1	cell					702:705	the cell	698:705	the cell	698:705	A pressurized atmosphere in the cell suppresses boiling of the volatile components, allowing the characterization of the structure-property relationships of the sample over a range of testing conditions (-5 to 150°C) which simulate industrial processing and storage conditions.					
32086941	6	134	theme	dispersions	1084:1094	arg1	gelatinization					1059:1072	In situ gelatinization	1051:1072	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate	1051:1229	In situ gelatinization of starch dispersions of varying starch particle weight fractions (ϕ) subjected to a high temperature (120°C) at elevated pressure and at a fixed shear rate is studied.					
32086941	7	135	theme	initial	1271:1277	arg1	slurry					1295:1300	an initial flowable starch slurry	1268:1300	an initial flowable starch slurry to a paste	1268:1311	A phase transition, from an initial flowable starch slurry to a paste, takes place during which the viscosity evolves by several orders of magnitude.					
32086941	12	136	theme	yield	2266:2270	arg1	stress					2272:2277	The yield stress	2262:2277	The yield stress	2262:2277	The yield stress and the paste viscosity both scale with starch particle weight fraction as (ϕ - ϕc ) m , where ϕc = 0.04 as no yield stress is observed for ϕ ≤ 0.04.					
32086941	3	137	theme	seals	503:507	arg1	free					471:474	free	471:474	free	471:474	We have developed a high-sensitivity, pressurized starch pasting cell (up to 5 bar) which utilizes a design free of mechanical bearings and seals, resulting in an order-of-magnitude improvement in torque sensitivity (1 μN.m in oscillatory and 10 μN.m in shear flows) compared to traditional pressure cells.					
32290896	10	0	theme	CHI	1813:1815	arg1	hippurate					1828:1836	hippurate	1828:1836	hippurate	1828:1836	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	0	theme	CHI	1813:1815	arg1	group					1817:1821	the CHI group	1809:1821	the CHI group	1809:1821	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	9	1	theme	arachidonic	1639:1649	arg1	acid					1651:1654	arachidonic acid	1639:1654	arachidonic acid	1639:1654	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	3	2	theme	21.65	592:596	arg1	±					598:598	±	598:598	±	598:598	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	2	3	theme	study	318:322	arg1	aim					306:308	The aim	302:308	The aim of this study	302:322	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	6	4	theme	daily	1135:1139	arg1	gain					1141:1144	average daily gain	1127:1144	average daily gain at 14, 42 and 56 days	1127:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	4	5	theme	control	730:736	arg1	groups					696:701	two groups	692:701	two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet	692:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	4	5	theme	control	730:736	arg1	group					744:748	the control (CON) group	726:748	the control (CON) group which received the basal diet	726:778	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	0	6	dep	performance	78:88	arg1	traits					107:112	quality traits	99:112	quality traits	99:112	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	9	7	theme	eicosapentaenoic	1660:1675	arg1	acid					1677:1680	eicosapentaenoic acid	1660:1680	eicosapentaenoic acid	1660:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	0	8	with	association	61:71	arg1	meat					94:97	meat	94:97	meat	94:97	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	0	8	with	association	61:71	arg1	performance					78:88	performance	78:88	performance	78:88	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	13	9	from	improvements	2529:2540	arg1	quality					2550:2556	meat quality	2545:2556	meat quality	2545:2556	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	13	9	from	improvements	2529:2540	arg1	profile					2517:2523	the meat FA profile	2505:2523	the meat FA profile	2505:2523	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	5	10	theme	body	962:965	arg1	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	10	theme	body	962:965	arg1	<					987:987	P < 0.05	985:992	P < 0.05	985:992	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	10	theme	body	962:965	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	8	11	theme	P	1451:1451	arg1	effect					1443:1448	no effect	1440:1448	no effect (P > 0.05)	1440:1459	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	8	11	theme	P	1451:1451	arg1	>					1453:1453	P > 0.05	1451:1458	P > 0.05	1451:1458	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	10	12	from	importance	1709:1718	arg1	score					1734:1738	projection score	1723:1738	projection score	1723:1738	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	9	13	theme	acid	1651:1654	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	13	theme	acid	1651:1654	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	6	14	theme	greater	1169:1175	arg1	efficiency					1182:1191	greater feed efficiency	1169:1191	greater feed efficiency at 28 days	1169:1202	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	7	15	theme	carcass	1268:1274	arg1	traits					1276:1281	the carcass traits	1264:1281	the carcass traits	1264:1281	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	6	16	from	days	1239:1242	arg1	gain					1141:1144	average daily gain	1127:1144	average daily gain at 14, 42 and 56 days	1127:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	16	from	days	1239:1242	arg1	efficiency					1182:1191	greater feed efficiency	1169:1191	greater feed efficiency at 28 days	1169:1202	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	16	from	days	1239:1242	arg1	conversation					1213:1224	feed conversation	1208:1224	feed conversation at 14 and 42 days in feedlot	1208:1253	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	16	from	days	1239:1242	arg1	feedlot					1247:1253	feedlot	1247:1253	feedlot	1247:1253	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	4	17	theme	basal	769:773	arg1	diet					775:778	the basal diet	765:778	the basal diet	765:778	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	7	18	dep	improved	1363:1370	arg1	<					1375:1375	P < 0.05	1373:1380	P < 0.05	1373:1380	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	13	19	theme	meat	2545:2548	arg1	quality					2550:2556	meat quality	2545:2556	meat quality	2545:2556	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	13	20	theme	FA	2514:2515	arg1	profile					2517:2523	the meat FA profile	2505:2523	the meat FA profile	2505:2523	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	11	21	theme	=	2168:2168	arg1	1.45					2170:2173	FC = 1.45	2165:2173	FC = 1.45	2165:2173	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	21	theme	=	2168:2168	arg1	hypoxanthine					2151:2162	hypoxanthine	2151:2162	hypoxanthine (FC = 1.45)	2151:2174	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	8	22	theme	meat	1468:1471	arg1	colour					1473:1478	the meat colour	1464:1478	the meat colour	1464:1478	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	6	23	theme	42	1236:1237	arg1	days					1239:1242	14 and 42 days	1229:1242	days	1239:1242	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	4	24	theme	CON	739:741	arg1	groups					696:701	two groups	692:701	two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet	692:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	4	24	theme	CON	739:741	arg1	group					744:748	the control (CON) group	726:748	the control (CON) group which received the basal diet	726:778	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	11	25	theme	FC	2032:2033	arg1	analysis					2036:2043	fold change (FC) analysis	2019:2043	fold change (FC) analysis	2019:2043	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	10	26	theme	variable	1700:1707	arg1	importance					1709:1718	the variable importance	1696:1718	the variable importance in projection score	1696:1738	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	3	27	theme	30-month-old	515:526	arg1	lambs					533:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	27	theme	30-month-old	515:526	arg1	Suffolk					545:551	half Suffolk	540:551	half Suffolk	540:551	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	27	theme	30-month-old	515:526	arg1	Dorper					562:567	half Dorper	557:567	half Dorper	557:567	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	6	28	theme	14	1229:1230	arg1	days					1239:1242	14 and 42 days	1229:1242	days	1239:1242	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	2	29	theme	serum	490:494	arg1	metabolome					496:505	serum metabolome	490:505	serum metabolome	490:505	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	5	30	theme	residual	1005:1012	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	30	theme	residual	1005:1012	arg1	intake					1019:1024	lower residual feed intake	999:1024	lower residual feed intake	999:1024	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	8	31	theme	quality	1490:1496	arg1	measurements					1498:1509	other quality measurements	1484:1509	other quality measurements	1484:1509	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	10	32	dep	glutamate	1972:1980	arg1	glycerol					1940:1947	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	glycerol	1940:1947	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	myo-inositol					1901:1912	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	myo-inositol	1901:1912	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	2-oxoglutarate					1915:1928	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	2-oxoglutarate	1915:1928	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	carnosine					1950:1958	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	carnosine	1950:1958	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	creatine					1882:1889	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	creatine	1882:1889	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	histidine					1961:1969	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	histidine	1961:1969	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	choline					1892:1898	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	choline	1892:1898	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	10	32	dep	glutamate	1972:1980	arg1	alanine					1931:1937	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	alanine	1931:1937	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	2	33	theme	acid	447:450	arg1	profile					457:463	meat fatty acid (FA) profile	436:463	meat fatty acid (FA) profile	436:463	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	3	34	theme	days	656:659	arg1	total					644:648	a total	642:648	a total of 70 days	642:659	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	35	theme	half	540:543	arg1	lambs					533:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	35	theme	half	540:543	arg1	Suffolk					545:551	half Suffolk	540:551	half Suffolk	540:551	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	6	36	from	days	1199:1202	arg1	gain					1141:1144	average daily gain	1127:1144	average daily gain at 14, 42 and 56 days	1127:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	36	from	days	1199:1202	arg1	efficiency					1182:1191	greater feed efficiency	1169:1191	greater feed efficiency at 28 days	1169:1202	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	36	from	days	1199:1202	arg1	conversation					1213:1224	feed conversation	1208:1224	feed conversation at 14 and 42 days in feedlot	1208:1253	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	2	37	theme	meat	436:439	arg1	FA					453:454	FA	453:454	FA	453:454	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	37	theme	meat	436:439	arg1	acid					447:450	meat fatty acid	436:450	meat fatty acid (FA) profile	436:463	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	7	38	dep	affected	1292:1299	arg1	>					1304:1304	P > 0.05	1302:1309	P > 0.05	1302:1309	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	6	39	theme	P	1077:1077	arg1	<					1079:1079	P < 0.05	1077:1084	P < 0.05	1077:1084	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	5	40	theme	greater	916:922	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	40	theme	greater	916:922	arg1	intake					948:953	DM intake	945:953	DM intake	945:953	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	40	theme	greater	916:922	arg1	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	40	theme	greater	916:922	arg1	intake					1019:1024	lower residual feed intake	999:1024	lower residual feed intake	999:1024	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	1	41	theme	natural	135:141	arg1	biopolymer					143:152	a natural biopolymer	133:152	a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics	133:299	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	1	41	theme	natural	135:141	arg1	Chitosan					115:122	Chitosan	115:122	Chitosan (CHI)	115:128	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	10	42	theme	important	1750:1758	arg1	metabolites					1760:1770	the most important metabolites	1741:1770	the most important metabolites	1741:1770	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	11	43	theme	=	2214:2214	arg1	FC					2211:2212	FC = 1.35	2211:2219	FC = 1.35	2211:2219	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	43	theme	=	2214:2214	arg1	malonate					2201:2208	malonate	2201:2208	malonate (FC = 1.35)	2201:2220	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	6	44	theme	greater	1068:1074	arg1	digestibility					1094:1106	a greater (P < 0.05) starch digestibility	1066:1106	a greater (P < 0.05) starch digestibility	1066:1106	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	45	dep	greater	1068:1074	arg1	<					1079:1079	P < 0.05	1077:1084	P < 0.05	1077:1084	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	4	46	theme	CHI	788:790	arg1	groups					696:701	two groups	692:701	two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet	692:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	4	46	theme	CHI	788:790	arg1	group					792:796	the CHI group	784:796	the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet	784:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	11	47	theme	change	2024:2029	arg1	analysis					2036:2043	fold change (FC) analysis	2019:2043	fold change (FC) analysis	2019:2043	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	9	48	theme	P	1569:1569	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	48	theme	P	1569:1569	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	2	49	theme	CHI	355:357	arg1	effects					344:350	the effects	340:350	the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome	340:505	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	0	50	theme	quality	99:105	arg1	traits					107:112	quality traits	99:112	quality traits	99:112	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	12	51	theme	muscle	2337:2342	arg1	metabolism					2344:2353	the muscle metabolism	2333:2353	the muscle metabolism	2333:2353	In conclusion, the present data showed that CHI changes the muscle metabolism improving muscle mass deposition, the lamb's performance and carcass dressing.					
32290896	0	52	theme	Serum	0:4	arg1	fingerprints					18:29	Serum metabolomic fingerprints	0:29	Serum metabolomic fingerprints of lambs	0:38	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	5	53	theme	final	935:939	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	53	theme	final	935:939	arg1	intake					948:953	DM intake	945:953	DM intake	945:953	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	53	theme	final	935:939	arg1	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	53	theme	final	935:939	arg1	intake					1019:1024	lower residual feed intake	999:1024	lower residual feed intake	999:1024	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	9	54	from	lambs	1534:1538	arg1	Meat					1512:1515	Meat	1512:1515	Meat from the CHI-fed lambs	1512:1538	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	4	55	dep	groups	696:701	arg1	groups					696:701	two groups	692:701	two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet	692:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	4	55	dep	groups	696:701	arg1	group					744:748	the control (CON) group	726:748	the control (CON) group which received the basal diet	726:778	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	4	55	dep	groups	696:701	arg1	group					792:796	the CHI group	784:796	the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet	784:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	1	56	theme	ruminant	264:271	arg1	diet					273:276	the ruminant diet	260:276	the ruminant diet	260:276	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	1	57	used	used	252:255	arg2	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	9	58	theme	acid	1594:1597	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	58	theme	acid	1594:1597	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	2	59	theme	lamb	362:365	arg1	traits					374:379	lamb growth traits	362:379	lamb growth traits	362:379	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	0	60	theme	lambs	34:38	arg1	fingerprints					18:29	Serum metabolomic fingerprints	0:29	Serum metabolomic fingerprints of lambs	0:38	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	5	61	theme	P	925:925	arg1	<					927:927	P < 0.05	925:932	P < 0.05	925:932	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	2	62	theme	fatty	418:422	arg1	nutrients					382:390	nutrients digestibility, muscle and fatty deposition	382:433	nutrients digestibility, muscle and fatty deposition	382:433	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	62	theme	fatty	418:422	arg1	deposition					424:433	fatty deposition	418:433	fatty deposition	418:433	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	nutrients					382:390	nutrients digestibility, muscle and fatty deposition	382:433	nutrients digestibility, muscle and fatty deposition	382:433	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	profile					457:463	meat fatty acid (FA) profile	436:463	meat fatty acid (FA) profile	436:463	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	digestibility					392:404	digestibility	392:404	digestibility	392:404	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	metabolome					496:505	serum metabolome	490:505	serum metabolome	490:505	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	muscle					407:412	muscle	407:412	muscle	407:412	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	traits					374:379	lamb growth traits	362:379	lamb growth traits	362:379	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	traits					479:484	meat quality traits	466:484	meat quality traits	466:484	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	63	from	effects	344:350	arg1	deposition					424:433	fatty deposition	418:433	fatty deposition	418:433	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	9	64	theme	linoleic	1600:1607	arg1	acid					1609:1612	linoleic acid	1600:1612	linoleic acid	1600:1612	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	6	65	theme	starch	1087:1092	arg1	digestibility					1094:1106	a greater (P < 0.05) starch digestibility	1066:1106	a greater (P < 0.05) starch digestibility	1066:1106	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	12	66	theme	muscle	2365:2370	arg1	deposition					2377:2386	muscle mass deposition	2365:2386	muscle mass deposition	2365:2386	In conclusion, the present data showed that CHI changes the muscle metabolism improving muscle mass deposition, the lamb's performance and carcass dressing.					
32290896	1	67	theme	digestive	209:217	arg1	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	7	68	theme	longissimus	1396:1406	arg1	area					1415:1418	longissimus muscle area	1396:1418	longissimus muscle area	1396:1418	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	3	69	theme	average	578:584	arg1	BW					586:587	an average BW	575:587	an average BW of 21.65 ± 0.86 kg	575:606	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	0	70	with	chitosan	44:51	arg1	meat					94:97	meat	94:97	meat	94:97	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	0	70	with	chitosan	44:51	arg1	performance					78:88	performance	78:88	performance	78:88	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	6	71	theme	14	1149:1150	arg1	days					1163:1166	14, 42 and 56 days	1149:1166	days	1163:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	3	72	theme	kg	605:606	arg1	BW					586:587	an average BW	575:587	an average BW of 21.65 ± 0.86 kg	575:606	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	4	73	theme	DM	866:867	arg1	g/kg					858:861	2 g/kg	856:861	2 g/kg of DM in the diet	856:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	11	74	theme	=	2093:2093	arg1	FC					2090:2091	FC = 1.51	2090:2098	FC = 1.51	2090:2098	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	74	theme	=	2093:2093	arg1	arginine					2080:2087	arginine	2080:2087	arginine (FC = 1.51)	2080:2099	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	6	75	theme	average	1127:1133	arg1	gain					1141:1144	average daily gain	1127:1144	average daily gain at 14, 42 and 56 days	1127:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	5	76	theme	final	956:960	arg1	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	76	theme	final	956:960	arg1	<					987:987	P < 0.05	985:992	P < 0.05	985:992	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	76	theme	final	956:960	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	7	77	theme	P	1373:1373	arg1	<					1375:1375	P < 0.05	1373:1380	P < 0.05	1373:1380	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	9	78	theme	acid	1677:1680	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	78	theme	acid	1677:1680	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	6	79	contain	had	1062:1064	arg1	Animals					1046:1052	Animals	1046:1052	Animals fed CHI	1046:1060	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	79	contain	had	1062:1064	arg2	efficiency					1182:1191	greater feed efficiency	1169:1191	greater feed efficiency at 28 days	1169:1202	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	79	contain	had	1062:1064	arg2	conversation					1213:1224	feed conversation	1208:1224	feed conversation at 14 and 42 days in feedlot	1208:1253	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	79	contain	had	1062:1064	arg2	digestibility					1094:1106	a greater (P < 0.05) starch digestibility	1066:1106	a greater (P < 0.05) starch digestibility	1066:1106	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	79	contain	had	1062:1064	arg2	gain					1141:1144	average daily gain	1127:1144	average daily gain at 14, 42 and 56 days	1127:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	11	80	theme	FC	2165:2166	arg1	1.45					2170:2173	FC = 1.45	2165:2173	FC = 1.45	2165:2173	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	80	theme	FC	2165:2166	arg1	hypoxanthine					2151:2162	hypoxanthine	2151:2162	hypoxanthine (FC = 1.45)	2151:2174	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	6	81	theme	feed	1177:1180	arg1	efficiency					1182:1191	greater feed efficiency	1169:1191	greater feed efficiency at 28 days	1169:1202	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	1	82	theme	antimicrobial	159:171	arg1	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	7	83	theme	CHI	1343:1345	arg1	supplementation					1347:1361	the CHI supplementation	1339:1361	the CHI supplementation	1339:1361	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	12	84	theme	carcass	2416:2422	arg1	dressing					2424:2431	carcass dressing	2416:2431	carcass dressing	2416:2431	In conclusion, the present data showed that CHI changes the muscle metabolism improving muscle mass deposition, the lamb's performance and carcass dressing.					
32290896	1	85	theme	anti-inflammatory	174:190	arg1	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	2	86	theme	quality	471:477	arg1	traits					479:484	meat quality traits	466:484	meat quality traits	466:484	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	3	87	theme	feedlot	623:629	arg1	system					631:636	a feedlot system	621:636	a feedlot system	621:636	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	88	theme	male	528:531	arg1	lambs					533:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	88	theme	male	528:531	arg1	Suffolk					545:551	half Suffolk	540:551	half Suffolk	540:551	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	88	theme	male	528:531	arg1	Dorper					562:567	half Dorper	557:567	half Dorper	557:567	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	6	89	theme	56	1160:1161	arg1	days					1163:1166	14, 42 and 56 days	1149:1166	days	1163:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	1	90	theme	antioxidant	193:203	arg1	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	9	91	contain	had	1540:1542	arg1	Meat					1512:1515	Meat	1512:1515	Meat from the CHI-fed lambs	1512:1538	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	91	contain	had	1540:1542	arg2	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	91	contain	had	1540:1542	arg2	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	8	92	theme	other	1484:1488	arg1	measurements					1498:1509	other quality measurements	1484:1509	other quality measurements	1484:1509	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	13	93	from	changes	2494:2500	arg1	quality					2550:2556	meat quality	2545:2556	meat quality	2545:2556	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	13	93	from	changes	2494:2500	arg1	profile					2517:2523	the meat FA profile	2505:2523	the meat FA profile	2505:2523	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	10	94	theme	malonate	1872:1879	arg1	glutamate					1972:1980	malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate	1872:1980	glutamate	1972:1980	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	5	95	theme	lower	999:1003	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	95	theme	lower	999:1003	arg1	intake					1019:1024	lower residual feed intake	999:1024	lower residual feed intake	999:1024	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	9	96	theme	CHI-fed	1526:1532	arg1	lambs					1534:1538	the CHI-fed lambs	1522:1538	the CHI-fed lambs	1522:1538	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	5	97	theme	feed	1014:1017	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	97	theme	feed	1014:1017	arg1	intake					1019:1024	lower residual feed intake	999:1024	lower residual feed intake	999:1024	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	12	98	theme	mass	2372:2375	arg1	deposition					2377:2386	muscle mass deposition	2365:2386	muscle mass deposition	2365:2386	In conclusion, the present data showed that CHI changes the muscle metabolism improving muscle mass deposition, the lamb's performance and carcass dressing.					
32290896	6	99	theme	feed	1208:1211	arg1	conversation					1213:1224	feed conversation	1208:1224	feed conversation at 14 and 42 days in feedlot	1208:1253	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	2	100	theme	fatty	441:445	arg1	FA					453:454	FA	453:454	FA	453:454	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	100	theme	fatty	441:445	arg1	acid					447:450	meat fatty acid	436:450	meat fatty acid (FA) profile	436:463	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	10	101	theme	projection	1723:1732	arg1	score					1734:1738	projection score	1723:1738	projection score	1723:1738	According to the variable importance in projection score, the most important metabolites to differentiate between the CON and the CHI group were hippurate, acetate, hypoxanthine, arginine, malonate, creatine, choline, myo-inositol, 2-oxoglutarate, alanine, glycerol, carnosine, histidine, glutamate and 3-hydroxyisobutyrate.					
32290896	5	102	theme	metabolic	967:975	arg1	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	102	theme	metabolic	967:975	arg1	<					987:987	P < 0.05	985:992	P < 0.05	985:992	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	102	theme	metabolic	967:975	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	4	103	from	g/kg	858:861	arg1	diet					876:879	the diet	872:879	the diet	872:879	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	5	104	contain	had	910:912	arg2	intake					1019:1024	lower residual feed intake	999:1024	lower residual feed intake	999:1024	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	104	contain	had	910:912	arg2	intake					948:953	DM intake	945:953	DM intake	945:953	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	104	contain	had	910:912	arg1	Lambs					882:886	Lambs	882:886	Lambs supplemented with CHI	882:908	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	104	contain	had	910:912	arg2	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	104	contain	had	910:912	arg2	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	9	105	theme	greater	1546:1552	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	105	theme	greater	1546:1552	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	11	106	theme	=	2142:2142	arg1	myo-inositol					2125:2136	myo-inositol	2125:2136	myo-inositol (FC = 1.48)	2125:2148	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	106	theme	=	2142:2142	arg1	FC					2139:2140	FC = 1.48	2139:2147	FC = 1.48	2139:2147	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	107	theme	fold	2019:2022	arg1	analysis					2036:2043	fold change (FC) analysis	2019:2043	fold change (FC) analysis	2019:2043	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	2	108	dep	nutrients	382:390	arg1	digestibility					392:404	digestibility	392:404	digestibility	392:404	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	108	dep	nutrients	382:390	arg1	nutrients					382:390	nutrients digestibility, muscle and fatty deposition	382:433	nutrients digestibility, muscle and fatty deposition	382:433	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	108	dep	nutrients	382:390	arg1	muscle					407:412	muscle	407:412	muscle	407:412	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	2	108	dep	nutrients	382:390	arg1	deposition					424:433	fatty deposition	418:433	fatty deposition	418:433	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	0	109	theme	metabolomic	6:16	arg1	fingerprints					18:29	Serum metabolomic fingerprints	0:29	Serum metabolomic fingerprints of lambs	0:38	Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits.					
32290896	6	110	from	days	1163:1166	arg1	gain					1141:1144	average daily gain	1127:1144	average daily gain at 14, 42 and 56 days	1127:1166	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	110	from	days	1163:1166	arg1	efficiency					1182:1191	greater feed efficiency	1169:1191	greater feed efficiency at 28 days	1169:1202	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	6	110	from	days	1163:1166	arg1	conversation					1213:1224	feed conversation	1208:1224	feed conversation at 14 and 42 days in feedlot	1208:1253	Animals fed CHI had a greater (P < 0.05) starch digestibility at 14 and 28 days, average daily gain at 14, 42 and 56 days, greater feed efficiency at 28 days and feed conversation at 14 and 42 days in feedlot.					
32290896	7	111	theme	P	1302:1302	arg1	>					1304:1304	P > 0.05	1302:1309	P > 0.05	1302:1309	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	13	112	theme	meat	2509:2512	arg1	profile					2517:2523	the meat FA profile	2505:2523	the meat FA profile	2505:2523	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	2	113	theme	meat	466:469	arg1	traits					479:484	meat quality traits	466:484	meat quality traits	466:484	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	5	114	dep	greater	916:922	arg1	<					927:927	P < 0.05	925:932	P < 0.05	925:932	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	9	115	theme	acid	1633:1636	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	115	theme	acid	1633:1636	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	13	116	from	alteration	2461:2470	arg1	metabolism					2482:2491	the FA metabolism	2475:2491	the FA metabolism	2475:2491	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	13	117	theme	FA	2479:2480	arg1	metabolism					2482:2491	the FA metabolism	2475:2491	the FA metabolism	2475:2491	In addition, CHI led to an alteration in the FA metabolism, changes in the meat FA profile and improvements in meat quality.					
32290896	4	118	theme	CHI	849:851	arg1	addition					837:844	the addition	833:844	the addition of CHI	833:851	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	9	119	theme	oleic-cis-9	1582:1592	arg1	acid					1594:1597	oleic-cis-9 acid	1582:1597	oleic-cis-9 acid	1582:1597	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	5	120	theme	CON	1035:1037	arg1	group					1039:1043	the CON group	1031:1043	the CON group	1031:1043	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	2	121	theme	growth	367:372	arg1	traits					374:379	lamb growth traits	362:379	lamb growth traits	362:379	The aim of this study was to evaluate the effects of CHI on lamb growth traits, nutrients digestibility, muscle and fatty deposition, meat fatty acid (FA) profile, meat quality traits and serum metabolome.					
32290896	5	122	theme	DM	945:946	arg1	BW					941:942	a greater (P < 0.05) final BW	914:942	a greater (P < 0.05) final BW	914:942	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	122	theme	DM	945:946	arg1	intake					948:953	DM intake	945:953	DM intake	945:953	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	11	123	theme	different	2251:2259	arg1	succinate					2057:2065	succinate	2057:2065	succinate (FC = 1.53)	2057:2077	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	hippurate					2102:2110	hippurate	2102:2110	hippurate (FC = 0.68)	2102:2122	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	arginine					2080:2087	arginine	2080:2087	arginine (FC = 1.51)	2080:2099	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	myo-inositol					2125:2136	myo-inositol	2125:2136	myo-inositol (FC = 1.48)	2125:2148	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	hypoxanthine					2151:2162	hypoxanthine	2151:2162	hypoxanthine (FC = 1.45)	2151:2174	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	malonate					2201:2208	malonate	2201:2208	malonate (FC = 1.35)	2201:2220	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	acetate					2177:2183	acetate	2177:2183	acetate (FC = 0.73)	2177:2195	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	123	theme	different	2251:2259	arg1	metabolites					2225:2235	metabolites	2225:2235	metabolites significantly different between groups	2225:2274	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	8	124	contain	had	1436:1438	arg2	>					1453:1453	P > 0.05	1451:1458	P > 0.05	1451:1458	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	8	124	contain	had	1436:1438	arg2	effect					1443:1448	no effect	1440:1448	no effect (P > 0.05)	1440:1459	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	8	124	contain	had	1436:1438	arg1	treatments					1425:1434	The treatments	1421:1434	The treatments	1421:1434	The treatments had no effect (P > 0.05) on the meat colour and other quality measurements.					
32290896	4	125	theme	basal	817:821	arg1	diet					823:826	the basal diet	813:826	the basal diet	813:826	The lambs were separated into two groups according to the diet: the control (CON) group which received the basal diet and the CHI group which received the basal diet with the addition of CHI as 2 g/kg of DM in the diet.					
32290896	11	126	theme	=	2116:2116	arg1	FC					2113:2114	FC = 0.68	2113:2121	FC = 0.68	2113:2121	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	126	theme	=	2116:2116	arg1	hippurate					2102:2110	hippurate	2102:2110	hippurate (FC = 0.68)	2102:2122	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	3	127	theme	half	557:560	arg1	lambs					533:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs	508:537	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	3	127	theme	half	557:560	arg1	Dorper					562:567	half Dorper	557:567	half Dorper	557:567	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	11	128	theme	=	2189:2189	arg1	FC					2186:2187	FC = 0.73	2186:2194	FC = 0.73	2186:2194	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	128	theme	=	2189:2189	arg1	acetate					2177:2183	acetate	2177:2183	acetate (FC = 0.73)	2177:2195	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	3	129	with	lambs	533:537	arg1	BW					586:587	an average BW	575:587	an average BW of 21.65 ± 0.86 kg	575:606	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	12	130	theme	present	2296:2302	arg1	data					2304:2307	the present data	2292:2307	the present data	2292:2307	In conclusion, the present data showed that CHI changes the muscle metabolism improving muscle mass deposition, the lamb's performance and carcass dressing.					
32290896	9	131	theme	acid	1609:1612	arg1	concentration					1554:1566	a greater concentration	1544:1566	a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid	1544:1680	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	9	131	theme	acid	1609:1612	arg1	<					1571:1571	P < 0.05	1569:1576	P < 0.05	1569:1576	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	1	132	with	biopolymer	143:152	arg1	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	9	133	theme	linolenic-trans-6	1615:1631	arg1	acid					1633:1636	linolenic-trans-6 acid	1615:1636	linolenic-trans-6 acid	1615:1636	Meat from the CHI-fed lambs had a greater concentration (P < 0.05) of oleic-cis-9 acid, linoleic acid, linolenic-trans-6 acid, arachidonic acid and eicosapentaenoic acid.					
32290896	1	134	theme	modulatory	219:228	arg1	effects					230:236	antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects	159:236	effects	230:236	Chitosan (CHI) is a natural biopolymer with antimicrobial, anti-inflammatory, antioxidant and digestive modulatory effects, which can be used in the ruminant diet to replace antibiotics.					
32290896	7	135	theme	muscle	1408:1413	arg1	area					1415:1418	longissimus muscle area	1396:1418	longissimus muscle area	1396:1418	Most of the carcass traits were not affected (P > 0.05) by the treatment; however, the CHI supplementation improved (P < 0.05) dressing and longissimus muscle area.					
32290896	11	136	theme	=	2071:2071	arg1	succinate					2057:2065	succinate	2057:2065	succinate (FC = 1.53)	2057:2077	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	11	136	theme	=	2071:2071	arg1	FC					2068:2069	FC = 1.53	2068:2076	FC = 1.53	2068:2076	Similarly, fold change (FC) analysis highlighted succinate (FC = 1.53), arginine (FC = 1.51), hippurate (FC = 0.68), myo-inositol (FC = 1.48), hypoxanthine (FC = 1.45), acetate (FC = 0.73) and malonate (FC = 1.35) as metabolites significantly different between groups.					
32290896	3	137	theme	0.86	600:603	arg1	±					598:598	±	598:598	±	598:598	Thirty 30-month-old male lambs, half Suffolk and half Dorper, with an average BW of 21.65 ± 0.86 kg, were fed in a feedlot system for a total of 70 days.					
32290896	5	138	theme	P	985:985	arg1	weight					977:982	final body metabolic weight	956:982	final body metabolic weight (P < 0.05)	956:993	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
32290896	5	138	theme	P	985:985	arg1	<					987:987	P < 0.05	985:992	P < 0.05	985:992	Lambs supplemented with CHI had a greater (P < 0.05) final BW, DM intake, final body metabolic weight (P < 0.05) and lower residual feed intake than the CON group.					
33007970	3	0	theme	potent	327:332	arg1	antibodies					346:355	potent anti-glycan antibodies	327:355	potent anti-glycan antibodies	327:355	Here, we show that potent anti-glycan antibodies can be engineered to acquire cancer therapeutic efficacy.					
33007970	5	1	theme	colon	664:668	arg1	lines					682:686	human pancreatic and colon cancer cell lines	643:686	lines	682:686	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	7	2	theme	major	1048:1052	arg1	advances					1069:1076	major methodological advances	1048:1076	major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics	1048:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	1	3	theme	carbohydrate	174:185	arg1	TACA					197:200	TACA	197:200	TACA	197:200	Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA).					
33007970	1	3	theme	carbohydrate	174:185	arg1	antigens					187:194	tumor-associated carbohydrate antigens	157:194	tumor-associated carbohydrate antigens (TACA)	157:201	Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA).					
33007970	3	4	theme	anti-glycan	334:344	arg1	antibodies					346:355	potent anti-glycan antibodies	327:355	potent anti-glycan antibodies	327:355	Here, we show that potent anti-glycan antibodies can be engineered to acquire cancer therapeutic efficacy.					
33007970	5	5	theme	cancer	670:675	arg1	lines					682:686	human pancreatic and colon cancer cell lines	643:686	lines	682:686	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	1	6	theme	antigens	187:194	arg1	expression					143:152	expression	143:152	expression of tumor-associated carbohydrate antigens (TACA)	143:201	Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA).					
33007970	7	7	theme	effective	922:930	arg1	antibodies					944:953	effective anti-glycan antibodies	922:953	effective anti-glycan antibodies	922:953	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	7	8	from	research	1127:1134	arg1	field					1143:1147	the field	1139:1147	the field of cancer theranostics	1139:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	5	9	theme	cell	677:680	arg1	lines					682:686	human pancreatic and colon cancer cell lines	643:686	lines	682:686	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	10	theme	improved	623:630	arg1	binding					632:638	improved binding	623:638	improved binding of human pancreatic and colon cancer cell lines	623:686	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	7	11	theme	anti-glycan	932:942	arg1	antibodies					944:953	effective anti-glycan antibodies	922:953	effective anti-glycan antibodies	922:953	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	7	12	theme	methodological	1054:1067	arg1	advances					1069:1076	major methodological advances	1048:1076	major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics	1048:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	5	13	theme	lines	682:686	arg1	efficacy					736:743	increased complement-dependent therapeutic efficacy	693:743	increased complement-dependent therapeutic efficacy	693:743	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	13	theme	lines	682:686	arg1	affinity					593:600	increased affinity	583:600	increased affinity	583:600	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	13	theme	lines	682:686	arg1	specificity					610:620	better specificity	603:620	better specificity	603:620	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	13	theme	lines	682:686	arg1	binding					632:638	improved binding	623:638	improved binding of human pancreatic and colon cancer cell lines	623:686	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	4	14	theme	surface	433:439	arg1	display					441:447	yeast surface display	427:447	yeast surface display	427:447	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	6	15	theme	Molecular	746:754	arg1	modeling					756:763	Molecular modeling	746:763	Molecular modeling	746:763	Molecular modeling explained the structural basis for improved antibody functionality at the molecular level.					
33007970	7	16	theme	selection	908:916	arg1	tools					866:870	These new tools	856:870	These new tools	856:870	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	4	17	from	SLea	516:519	arg1	colon					533:537	colon	533:537	colon	533:537	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	4	17	from	SLea	516:519	arg1	cancers					554:560	pancreatic cancers	543:560	pancreatic cancers	543:560	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	4	18	theme	yeast	427:431	arg1	display					441:447	yeast surface display	427:447	yeast surface display	427:447	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	1	19	theme	Glycosylation	80:92	arg1	patterns					94:101	Glycosylation patterns	80:101	Glycosylation patterns	80:101	Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA).					
33007970	7	20	theme	theranostics	1159:1170	arg1	field					1143:1147	the field	1139:1147	the field of cancer theranostics	1139:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	5	21	theme	increased	583:591	arg1	affinity					593:600	increased affinity	583:600	increased affinity	583:600	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	2	22	theme	cancer	292:297	arg1	therapy					299:305	clinical cancer therapy	283:305	clinical cancer therapy	283:305	While promising, currently available anti-glycan antibodies are not useful for clinical cancer therapy.					
33007970	7	23	theme	evolution	894:902	arg1	tools					866:870	These new tools	856:870	These new tools	856:870	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	6	24	from	level	849:853	arg1	basis					790:794	the structural basis	775:794	the structural basis for improved antibody functionality at the molecular level	775:853	Molecular modeling explained the structural basis for improved antibody functionality at the molecular level.					
33007970	4	25	theme	TACA	511:514	arg1	CA19-9					522:527	CA19-9	522:527	CA19-9	522:527	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	4	25	theme	TACA	511:514	arg1	SLea					516:519	the TACA SLea	507:519	the TACA SLea (CA19-9) in colon and pancreatic cancers	507:560	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	5	26	theme	pancreatic	649:658	arg1	efficacy					736:743	increased complement-dependent therapeutic efficacy	693:743	increased complement-dependent therapeutic efficacy	693:743	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	26	theme	pancreatic	649:658	arg1	affinity					593:600	increased affinity	583:600	increased affinity	583:600	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	26	theme	pancreatic	649:658	arg1	specificity					610:620	better specificity	603:620	better specificity	603:620	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	26	theme	pancreatic	649:658	arg1	binding					632:638	improved binding	623:638	improved binding of human pancreatic and colon cancer cell lines	623:686	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	7	27	theme	therapy	1003:1009	arg1	mechanisms					982:991	the mechanisms	978:991	the mechanisms of cancer therapy targeting glycosylation	978:1033	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	7	28	theme	cancer	996:1001	arg1	therapy					1003:1009	cancer therapy	996:1009	cancer therapy targeting glycosylation	996:1033	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	0	29	theme	Directed	0:7	arg1	Evolution					9:17	Directed Evolution	0:17	Directed Evolution of Therapeutic Antibodies	0:43	Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in Cancer.					
33007970	7	30	from	field	1143:1147	arg1	avenues					1116:1122	innovative avenues	1105:1122	innovative avenues of research in the field of cancer theranostics	1105:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	7	31	theme	cancer	1152:1157	arg1	theranostics					1159:1170	cancer theranostics	1152:1170	cancer theranostics	1152:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	7	32	theme	directed	875:882	arg1	evolution					894:902	directed molecular evolution	875:902	directed molecular evolution	875:902	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	3	33	theme	cancer	386:391	arg1	efficacy					405:412	cancer therapeutic efficacy	386:412	cancer therapeutic efficacy	386:412	Here, we show that potent anti-glycan antibodies can be engineered to acquire cancer therapeutic efficacy.					
33007970	7	34	theme	new	862:864	arg1	tools					866:870	These new tools	856:870	These new tools	856:870	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	5	35	theme	therapeutic	724:734	arg1	efficacy					736:743	increased complement-dependent therapeutic efficacy	693:743	increased complement-dependent therapeutic efficacy	693:743	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	5	36	theme	better	603:608	arg1	specificity					610:620	better specificity	603:620	better specificity	603:620	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	7	37	theme	molecular	884:892	arg1	evolution					894:902	directed molecular evolution	875:902	directed molecular evolution	875:902	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	3	38	theme	therapeutic	393:403	arg1	efficacy					405:412	cancer therapeutic efficacy	386:412	cancer therapeutic efficacy	386:412	Here, we show that potent anti-glycan antibodies can be engineered to acquire cancer therapeutic efficacy.					
33007970	0	39	theme	Antibodies	34:43	arg1	Evolution					9:17	Directed Evolution	0:17	Directed Evolution of Therapeutic Antibodies	0:43	Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in Cancer.					
33007970	7	40	theme	research	1127:1134	arg1	avenues					1116:1122	innovative avenues	1105:1122	innovative avenues of research in the field of cancer theranostics	1105:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	0	41	theme	Therapeutic	22:32	arg1	Antibodies					34:43	Therapeutic Antibodies	22:43	Therapeutic Antibodies	22:43	Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in Cancer.					
33007970	6	42	theme	antibody	809:816	arg1	functionality					818:830	improved antibody functionality	800:830	improved antibody functionality	800:830	Molecular modeling explained the structural basis for improved antibody functionality at the molecular level.					
33007970	7	43	theme	innovative	1105:1114	arg1	avenues					1116:1122	innovative avenues	1105:1122	innovative avenues of research in the field of cancer theranostics	1105:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
33007970	4	44	theme	therapeutic	476:486	arg1	antibodies					488:497	therapeutic antibodies	476:497	therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers	476:560	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	2	45	theme	anti-glycan	241:251	arg1	antibodies					253:262	currently available anti-glycan antibodies	221:262	currently available anti-glycan antibodies	221:262	While promising, currently available anti-glycan antibodies are not useful for clinical cancer therapy.					
33007970	5	46	theme	Elite	563:567	arg1	clones					569:574	Elite clones	563:574	Elite clones	563:574	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	2	47	theme	available	231:239	arg1	antibodies					253:262	currently available anti-glycan antibodies	221:262	currently available anti-glycan antibodies	221:262	While promising, currently available anti-glycan antibodies are not useful for clinical cancer therapy.					
33007970	4	48	theme	pancreatic	543:552	arg1	cancers					554:560	pancreatic cancers	543:560	pancreatic cancers	543:560	We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19-9) in colon and pancreatic cancers.					
33007970	5	49	theme	increased	693:701	arg1	efficacy					736:743	increased complement-dependent therapeutic efficacy	693:743	increased complement-dependent therapeutic efficacy	693:743	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	6	50	theme	molecular	839:847	arg1	level					849:853	the molecular level	835:853	the molecular level	835:853	Molecular modeling explained the structural basis for improved antibody functionality at the molecular level.					
33007970	2	51	theme	clinical	283:290	arg1	therapy					299:305	clinical cancer therapy	283:305	clinical cancer therapy	283:305	While promising, currently available anti-glycan antibodies are not useful for clinical cancer therapy.					
33007970	6	52	theme	improved	800:807	arg1	functionality					818:830	improved antibody functionality	800:830	improved antibody functionality	800:830	Molecular modeling explained the structural basis for improved antibody functionality at the molecular level.					
33007970	5	53	theme	complement-dependent	703:722	arg1	efficacy					736:743	increased complement-dependent therapeutic efficacy	693:743	increased complement-dependent therapeutic efficacy	693:743	Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy.					
33007970	6	54	theme	structural	779:788	arg1	basis					790:794	the structural basis	775:794	the structural basis for improved antibody functionality at the molecular level	775:853	Molecular modeling explained the structural basis for improved antibody functionality at the molecular level.					
33007970	1	55	theme	tumor-associated	157:172	arg1	TACA					197:200	TACA	197:200	TACA	197:200	Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA).					
33007970	1	55	theme	tumor-associated	157:172	arg1	antigens					187:194	tumor-associated carbohydrate antigens	157:194	tumor-associated carbohydrate antigens (TACA)	157:201	Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA).					
33007970	7	56	from	avenues	1116:1122	arg1	field					1143:1147	the field	1139:1147	the field of cancer theranostics	1139:1170	These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.					
32754876	9	0	theme	wall	1315:1318	arg1	modifications					1320:1332	cell wall modifications	1310:1332	cell wall modifications	1310:1332	Supporting these findings, transcriptomics identified changes in genes involved in cell wall modifications.					
32754876	7	1	theme	different	1024:1032	arg1	levels					1034:1039	different levels	1024:1039	different levels of sucrose loss	1024:1055	We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage.					
32754876	3	2	theme	extended	512:519	arg1	time					521:524	an extended time	509:524	an extended time	509:524	Since its processing is increasingly centralized, storage of beet roots over an extended time has become necessary.					
32754876	11	3	theme	sucrose	1721:1727	arg1	loss					1729:1732	sucrose loss	1721:1732	sucrose loss	1721:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	12	4	theme	pathogen	1937:1944	arg1	resistance					1946:1955	pathogen resistance	1937:1955	pathogen resistance	1937:1955	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	5	5	theme	cell	776:779	arg1	composition					786:796	cell wall composition	776:796	cell wall composition	776:796	This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute.					
32754876	10	6	theme	response	1493:1500	arg1	category					1473:1480	the category	1469:1480	the category of defense response	1469:1500	After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway.					
32754876	4	7	theme	major	581:585	arg1	loss					556:559	Sucrose loss	548:559	Sucrose loss during storage	548:574	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	4	7	theme	major	581:585	arg1	concern					587:593	a major concern	579:593	a major concern for the sugar industry	579:616	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	8	8	theme	cell	1192:1195	arg1	area					1197:1200	a smaller cell area	1182:1200	a smaller cell area	1182:1200	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	11	9	theme	network	1623:1629	arg1	analysis					1631:1638	gene co-expression network analysis	1604:1638	gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss	1604:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	12	10	theme	beet	1798:1801	arg1	roots					1803:1807	sugar beet roots	1792:1807	sugar beet roots	1792:1807	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	2	11	theme	sugar	323:327	arg1	cane					329:332	sugar cane	323:332	sugar cane	323:332	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	10	12	theme	expressed	1386:1394	arg1	genes					1396:1400	over 900 differentially expressed genes	1362:1400	over 900 differentially expressed genes	1362:1400	After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway.					
32754876	5	13	theme	ongoing	740:746	arg1	respiration					748:758	ongoing respiration	740:758	ongoing respiration	740:758	This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute.					
32754876	6	14	theme	molecular	908:916	arg1	mechanisms					918:927	the underlying molecular mechanisms	893:927	the underlying molecular mechanisms	893:927	While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered.					
32754876	6	14	theme	molecular	908:916	arg1	undiscovered					943:954	undiscovered	943:954	undiscovered	943:954	While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered.					
32754876	12	15	theme	pathways	1865:1872	arg1	characterization					1841:1856	the characterization	1837:1856	the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties	1837:1978	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	11	16	theme	sugar	1698:1702	arg1	accumulation					1704:1715	invert sugar accumulation	1691:1715	invert sugar accumulation	1691:1715	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	6	17	theme	underlying	897:906	arg1	mechanisms					918:927	the underlying molecular mechanisms	893:927	the underlying molecular mechanisms	893:927	While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered.					
32754876	6	17	theme	underlying	897:906	arg1	undiscovered					943:954	undiscovered	943:954	undiscovered	943:954	While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered.					
32754876	12	18	used	used	1910:1913	arg2	genes					1882:1886	hub genes	1878:1886	hub genes	1878:1886	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	18	used	used	1910:1913	arg2	markers					1918:1924	markers	1918:1924	markers	1918:1924	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	18	used	used	1910:1913	arg2	pathways					1865:1872	key pathways	1861:1872	key pathways	1861:1872	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	1	19	theme	sucrose	187:193	arg1	loss					195:198	sucrose loss	187:198	sucrose loss	187:198	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	5	20	theme	pathogen	802:809	arg1	infestation					811:821	pathogen infestation	802:821	pathogen infestation	802:821	This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute.					
32754876	11	21	theme	accumulation	1704:1715	arg1	drivers					1680:1686	main drivers	1675:1686	main drivers of invert sugar accumulation and sucrose loss	1675:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	11	21	theme	accumulation	1704:1715	arg1	genes					1650:1654	hub genes	1646:1654	hub genes	1646:1654	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	11	22	theme	invert	1691:1696	arg1	accumulation					1704:1715	invert sugar accumulation	1691:1715	invert sugar accumulation	1691:1715	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	11	23	theme	co-expression	1609:1621	arg1	analysis					1631:1638	gene co-expression network analysis	1604:1638	gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss	1604:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	1	24	theme	integrative	83:93	arg1	approach					122:129	An integrative comparative transcriptomic approach	80:129	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage	80:213	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	1	25	theme	loss	195:198	arg1	amount					177:182	different amount	167:182	different amount of sucrose loss	167:198	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	1	25	theme	loss	195:198	arg1	loss					195:198	sucrose loss	187:198	sucrose loss	187:198	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	0	26	from	impact	46:51	arg1	beet					62:65	sugar beet storability	56:77	sugar beet storability	56:77	Integrative transcriptomics reveals genotypic impact on sugar beet storability.					
32754876	8	27	theme	storable	1104:1111	arg1	varieties					1113:1121	well storable varieties	1099:1121	well storable varieties	1099:1121	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	7	28	theme	integrative	968:978	arg1	transcriptomics					980:994	integrative transcriptomics	968:994	integrative transcriptomics	968:994	We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage.					
32754876	1	29	theme	comparative	95:105	arg1	approach					122:129	An integrative comparative transcriptomic approach	80:129	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage	80:213	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	11	30	theme	loss	1729:1732	arg1	drivers					1680:1686	main drivers	1675:1686	main drivers of invert sugar accumulation and sucrose loss	1675:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	11	30	theme	loss	1729:1732	arg1	genes					1650:1654	hub genes	1646:1654	hub genes	1646:1654	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	4	31	theme	sugar	653:657	arg1	accumulation					630:641	the accumulation	626:641	the accumulation of invert sugar and byproducts	626:672	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	0	32	theme	Integrative	0:10	arg1	transcriptomics					12:26	Integrative transcriptomics	0:26	Integrative transcriptomics	0:26	Integrative transcriptomics reveals genotypic impact on sugar beet storability.					
32754876	5	33	from	changes	765:771	arg1	composition					786:796	cell wall composition	776:796	cell wall composition	776:796	This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute.					
32754876	5	33	from	changes	765:771	arg1	infestation					811:821	pathogen infestation	802:821	pathogen infestation	802:821	This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute.					
32754876	1	34	theme	transcriptomic	107:120	arg1	approach					122:129	An integrative comparative transcriptomic approach	80:129	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage	80:213	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	10	35	theme	storage	1353:1359	arg1	13 weeks					1341:1348	13 weeks	1341:1348	13 weeks of storage	1341:1359	After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway.					
32754876	12	36	theme	storage	1961:1967	arg1	properties					1969:1978	storage properties	1961:1978	storage properties	1961:1978	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	0	37	theme	genotypic	36:44	arg1	impact					46:51	genotypic impact	36:51	genotypic impact on sugar beet storability	36:77	Integrative transcriptomics reveals genotypic impact on sugar beet storability.					
32754876	11	38	theme	gene	1604:1607	arg1	analysis					1631:1638	gene co-expression network analysis	1604:1638	gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss	1604:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	10	39	theme	defense	1485:1491	arg1	response					1493:1500	defense response	1485:1500	defense response	1485:1500	After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway.					
32754876	8	40	theme	thinner	1209:1215	arg1	periderm					1217:1224	a thinner periderm	1207:1224	a thinner periderm	1207:1224	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	12	41	theme	genes	1882:1886	arg1	characterization					1841:1856	the characterization	1837:1856	the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties	1837:1978	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	42	from	changes	1781:1787	arg1	roots					1803:1807	sugar beet roots	1792:1807	sugar beet roots	1792:1807	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	1	43	theme	genotype-specific	224:240	arg1	genes					254:258	genotype-specific main driver genes	224:258	genotype-specific main driver genes	224:258	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	5	44	theme	wall	781:784	arg1	composition					786:796	cell wall composition	776:796	cell wall composition	776:796	This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute.					
32754876	2	45	theme	sugar	360:364	arg1	crops					366:370	the most important sugar crops	341:370	the most important sugar crops accounting for about 15% of the sucrose produced worldwide	341:429	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	10	46	theme	carbohydrate	1514:1525	arg1	metabolism					1527:1536	carbohydrate metabolism	1514:1536	carbohydrate metabolism	1514:1536	After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway.					
32754876	1	47	theme	main	242:245	arg1	genes					254:258	genotype-specific main driver genes	224:258	genotype-specific main driver genes	224:258	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	4	48	theme	invert	646:651	arg1	sugar					653:657	invert sugar	646:657	invert sugar	646:657	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	2	49	theme	worldwide	421:429	arg1	worldwide					421:429	the sucrose produced worldwide	400:429	the sucrose produced worldwide	400:429	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	2	49	theme	worldwide	421:429	arg1	%					395:395	about 15%	387:395	about 15% of the sucrose produced worldwide	387:429	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	4	50	theme	byproducts	663:672	arg1	accumulation					630:641	the accumulation	626:641	the accumulation of invert sugar and byproducts	626:672	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	2	51	dep	produced	412:419	arg1	sucrose					404:410	sucrose	404:410	sucrose	404:410	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	11	52	theme	hub	1646:1648	arg1	drivers					1680:1686	main drivers	1675:1686	main drivers of invert sugar accumulation and sucrose loss	1675:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	11	52	theme	hub	1646:1648	arg1	genes					1650:1654	hub genes	1646:1654	hub genes	1646:1654	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	1	53	theme	driver	247:252	arg1	genes					254:258	genotype-specific main driver genes	224:258	genotype-specific main driver genes	224:258	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	8	54	theme	higher	1147:1152	arg1	number					1154:1159	a higher number	1145:1159	a higher number of parenchyma cells	1145:1179	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	12	55	theme	hub	1878:1880	arg1	markers					1918:1924	markers	1918:1924	markers	1918:1924	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	55	theme	hub	1878:1880	arg1	pathways					1865:1872	key pathways	1861:1872	key pathways	1861:1872	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	55	theme	hub	1878:1880	arg1	genes					1882:1886	hub genes	1878:1886	hub genes	1878:1886	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	10	56	theme	phenylpropanoid	1546:1560	arg1	pathway					1562:1568	the phenylpropanoid pathway	1542:1568	the phenylpropanoid pathway	1542:1568	After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway.					
32754876	2	57	theme	crops	366:370	arg1	one					334:336	one	334:336	one	334:336	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	2	57	theme	crops	366:370	arg1	crops					366:370	the most important sugar crops	341:370	the most important sugar crops accounting for about 15% of the sucrose produced worldwide	341:429	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	0	58	theme	sugar	56:60	arg1	beet					62:65	sugar beet storability	56:77	sugar beet storability	56:77	Integrative transcriptomics reveals genotypic impact on sugar beet storability.					
32754876	3	59	theme	beet	493:496	arg1	roots					498:502	beet roots	493:502	beet roots	493:502	Since its processing is increasingly centralized, storage of beet roots over an extended time has become necessary.					
32754876	7	60	theme	sucrose	1044:1050	arg1	loss					1052:1055	sucrose loss	1044:1055	sucrose loss	1044:1055	We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage.					
32754876	1	61	theme	sugar	138:142	arg1	varieties					149:157	six sugar beet varieties	134:157	six sugar beet varieties showing different amount of sucrose loss during storage	134:213	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	1	62	from	approach	122:129	arg1	varieties					149:157	six sugar beet varieties	134:157	six sugar beet varieties showing different amount of sucrose loss during storage	134:213	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	4	63	theme	Sucrose	548:554	arg1	loss					556:559	Sucrose loss	548:559	Sucrose loss during storage	548:574	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	4	63	theme	Sucrose	548:554	arg1	concern					587:593	a major concern	579:593	a major concern for the sugar industry	579:616	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	9	64	from	changes	1281:1287	arg1	genes					1292:1296	genes	1292:1296	genes involved in cell wall modifications	1292:1332	Supporting these findings, transcriptomics identified changes in genes involved in cell wall modifications.					
32754876	12	65	theme	sugar	1792:1796	arg1	beet					1798:1801	sugar beet	1792:1801	sugar beet roots	1792:1807	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	4	66	theme	sucrose	690:696	arg1	manufacturing					698:710	sucrose manufacturing	690:710	sucrose manufacturing	690:710	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	1	67	theme	beet	144:147	arg1	varieties					149:157	six sugar beet varieties	134:157	six sugar beet varieties showing different amount of sucrose loss during storage	134:213	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	12	68	theme	key	1861:1863	arg1	markers					1918:1924	markers	1918:1924	markers	1918:1924	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	68	theme	key	1861:1863	arg1	pathways					1865:1872	key pathways	1861:1872	key pathways	1861:1872	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	12	68	theme	key	1861:1863	arg1	genes					1882:1886	hub genes	1878:1886	hub genes	1878:1886	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	7	69	theme	loss	1052:1055	arg1	levels					1034:1039	different levels	1024:1039	different levels of sucrose loss	1024:1055	We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage.					
32754876	2	70	theme	Sugar	301:305	arg1	beet					307:310	Sugar beet	301:310	Sugar beet	301:310	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	3	71	theme	roots	498:502	arg1	storage					482:488	storage	482:488	storage of beet roots over an extended time	482:524	Since its processing is increasingly centralized, storage of beet roots over an extended time has become necessary.					
32754876	2	72	theme	important	350:358	arg1	crops					366:370	the most important sugar crops	341:370	the most important sugar crops accounting for about 15% of the sucrose produced worldwide	341:429	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	8	73	theme	cells	1175:1179	arg1	area					1197:1200	a smaller cell area	1182:1200	a smaller cell area	1182:1200	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	8	73	theme	cells	1175:1179	arg1	periderm					1217:1224	a thinner periderm	1207:1224	a thinner periderm	1207:1224	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	8	73	theme	cells	1175:1179	arg1	number					1154:1159	a higher number	1145:1159	a higher number of parenchyma cells	1145:1179	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	2	74	theme	produced	412:419	arg1	worldwide					421:429	the sucrose produced worldwide	400:429	the sucrose produced worldwide	400:429	Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide.					
32754876	11	75	theme	main	1675:1678	arg1	drivers					1680:1686	main drivers	1675:1686	main drivers of invert sugar accumulation and sucrose loss	1675:1732	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	11	75	theme	main	1675:1678	arg1	genes					1650:1654	hub genes	1646:1654	hub genes	1646:1654	These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss.					
32754876	12	76	theme	transcriptional	1765:1779	arg1	changes					1781:1787	transcriptional changes	1765:1787	transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties	1765:1978	Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.					
32754876	8	77	theme	parenchyma	1164:1173	arg1	cells					1175:1179	parenchyma cells	1164:1179	parenchyma cells	1164:1179	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
32754876	9	78	theme	cell	1310:1313	arg1	modifications					1320:1332	cell wall modifications	1310:1332	cell wall modifications	1310:1332	Supporting these findings, transcriptomics identified changes in genes involved in cell wall modifications.					
32754876	4	79	theme	sugar	603:607	arg1	industry					609:616	the sugar industry	599:616	the sugar industry	599:616	Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing.					
32754876	1	80	theme	different	167:175	arg1	amount					177:182	different amount	167:182	different amount of sucrose loss	167:198	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	1	80	theme	different	167:175	arg1	loss					195:198	sucrose loss	187:198	sucrose loss	187:198	An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability.					
32754876	8	81	theme	smaller	1184:1190	arg1	area					1197:1200	a smaller cell area	1182:1200	a smaller cell area	1182:1200	Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm.					
33142658	1	0	theme	value-adding	158:169	arg1	materials					171:179	value-adding materials	158:179	value-adding materials	158:179	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	1	1	theme	great	246:250	arg1	interest					261:268	great research interest	246:268	great research interest	246:268	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	2	2	theme	composite	457:465	arg1	membranes					467:475	composite membranes	457:475	composite membranes	457:475	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	3	3	theme	moisture	639:646	arg1	content					648:654	its moisture content	635:654	its moisture content	635:654	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	5	4	theme	100	977:979	arg1	%					981:981	%	981:981	%	981:981	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	2	5	theme	dispersion-casting	331:348	arg1	process					350:356	a simple dispersion-casting process	322:356	a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes	322:475	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	1	6	theme	research	252:259	arg1	interest					261:268	great research interest	246:268	great research interest	246:268	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	0	7	theme	blocking	108:115	arg1	membranes					117:125	UV blocking membranes	105:125	UV blocking membranes	105:125	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	5	8	theme	LS	1058:1059	arg1	content					1061:1067	the highest LS content	1046:1067	the highest LS content	1046:1067	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	3	9	theme	LS	511:512	arg1	dispersions					514:524	LS dispersions	511:524	LS dispersions	511:524	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	0	10	theme	UV	105:106	arg1	membranes					117:125	UV blocking membranes	105:125	UV blocking membranes	105:125	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	4	11	from	effect	791:796	arg1	properties					816:825	the mechanical properties	801:825	the mechanical properties of the final membranes	801:848	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	4	12	theme	mechanical	805:814	arg1	properties					816:825	the mechanical properties	801:825	the mechanical properties of the final membranes	801:848	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	5	13	dep	described	909:917	arg1	both					1011:1014	both	1011:1014	both	1011:1014	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	1	14	theme	core	186:189	arg1	objective					191:199	a core objective	184:199	a core objective	184:199	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	1	14	theme	core	186:189	arg1	use					132:134	The use	128:134	The use of biomass to produce value-adding materials	128:179	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	2	15	attach	present	309:315	arg2	we					306:307	we	306:307	we	306:307	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	15	attach	present	309:315	arg1	context					297:303	this context	292:303	this context	292:303	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	16	from	process	350:356	arg1	membranes					467:475	composite membranes	457:475	composite membranes	457:475	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	17	theme	nanofibrils	389:399	arg1	consolidation					362:374	consolidation	362:374	consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2	362:452	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	18	theme	-rich	428:432	arg1	nanofibrils					389:399	cellulose nanofibrils	379:399	cellulose nanofibrils (CNF)	379:405	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	18	theme	-rich	428:432	arg1	bio-waste					434:442	bio-waste	434:442	bio-waste	434:442	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	3	19	theme	ζ	538:538	arg1	potential					540:548	the ζ potential	534:548	the ζ potential	534:548	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	2	20	theme	cellulose	379:387	arg1	nanofibrils					389:399	cellulose nanofibrils	379:399	cellulose nanofibrils (CNF)	379:405	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	20	theme	cellulose	379:387	arg1	CaCl2					448:452	CaCl2	448:452	CaCl2	448:452	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	20	theme	cellulose	379:387	arg1	bio-waste					434:442	bio-waste	434:442	bio-waste	434:442	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	2	20	theme	cellulose	379:387	arg1	CNF					402:404	CNF	402:404	CNF	402:404	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	3	21	from	deposition	672:681	arg1	CNFs					686:689	CNFs	686:689	CNFs	686:689	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	1	22	theme	recent	273:278	arg1	decades					280:286	recent decades	273:286	recent decades	273:286	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	4	23	theme	adverse	783:789	arg1	effect					791:796	an adverse effect	780:796	an adverse effect on the mechanical properties of the final membranes	780:848	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	5	24	theme	retardancy	962:971	arg1	terms					922:926	terms	922:926	terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity	922:1008	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	5	25	theme	highest	1050:1056	arg1	content					1061:1067	the highest LS content	1046:1067	the highest LS content	1046:1067	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	0	26	theme	nanofibrils	27:37	arg1	Consolidation					0:12	Consolidation	0:12	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.	0:126	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	3	27	theme	dispersion	706:715	arg1	phase					717:721	the dispersion phase	702:721	the dispersion phase	702:721	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	4	28	theme	CNF	761:763	arg1	dispersion					765:774	CNF dispersion	761:774	CNF dispersion	761:774	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	5	29	theme	composite	880:888	arg1	membranes					890:898	the new composite membranes	872:898	the new composite membranes	872:898	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	0	30	theme	cellulose	17:25	arg1	nanofibrils					27:37	cellulose nanofibrils	17:37	cellulose nanofibrils	17:37	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	5	31	theme	electronic	1086:1095	arg1	resistance					1097:1106	high electronic resistance	1081:1106	high electronic resistance	1081:1106	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	5	32	theme	new	876:878	arg1	membranes					890:898	the new composite membranes	872:898	the new composite membranes	872:898	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	5	33	theme	UVA/UVB	983:989	arg1	capacity					1001:1008	100 % UVA/UVB shielding capacity	977:1008	100 % UVA/UVB shielding capacity	977:1008	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	5	34	theme	membranes	890:898	arg1	effectiveness					855:867	The effectiveness	851:867	The effectiveness of the new composite membranes	851:898	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	0	35	theme	lignosulphonate	44:58	arg1	bio-waste					60:68	lignosulphonate bio-waste	44:68	lignosulphonate bio-waste	44:68	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	5	36	theme	shielding	991:999	arg1	capacity					1001:1008	100 % UVA/UVB shielding capacity	977:1008	100 % UVA/UVB shielding capacity	977:1008	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	1	37	theme	circular	208:215	arg1	economy					217:223	the circular economy	204:223	the circular economy	204:223	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	5	38	theme	capacity	1001:1008	arg1	terms					922:926	terms	922:926	terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity	922:1008	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	4	39	theme	CaCl2	752:756	arg1	Addition					724:731	Addition	724:731	Addition of both the LS and CaCl2 to CNF dispersion	724:774	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	1	40	theme	economy	217:223	arg1	objective					191:199	a core objective	184:199	a core objective	184:199	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	1	40	theme	economy	217:223	arg1	use					132:134	The use	128:134	The use of biomass to produce value-adding materials	128:179	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	5	41	theme	high	1081:1084	arg1	resistance					1097:1106	high electronic resistance	1081:1106	high electronic resistance	1081:1106	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	3	42	theme	LS	669:670	arg1	deposition					672:681	LS deposition	669:681	LS deposition on CNFs	669:689	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	2	43	theme	simple	324:329	arg1	process					350:356	a simple dispersion-casting process	322:356	a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes	322:475	In this context, we present here a simple dispersion-casting process for consolidation of cellulose nanofibrils (CNF), lignosulphonate (LS)-rich bio-waste and CaCl2 in composite membranes.					
33142658	5	44	theme	flame	956:960	arg1	retardancy					962:971	their passive (charring) flame retardancy	931:971	their passive (charring) flame retardancy	931:971	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	3	45	theme	due	551:553	arg1	potential					540:548	the ζ potential	534:548	the ζ potential	534:548	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	5	46	theme	passive	937:943	arg1	retardancy					962:971	their passive (charring) flame retardancy	931:971	their passive (charring) flame retardancy	931:971	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	4	47	theme	membranes	840:848	arg1	properties					816:825	the mechanical properties	801:825	the mechanical properties of the final membranes	801:848	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	5	48	theme	charring	946:953	arg1	retardancy					962:971	their passive (charring) flame retardancy	931:971	their passive (charring) flame retardancy	931:971	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	0	49	with	Consolidation	0:12	arg1	bio-waste					60:68	lignosulphonate bio-waste	44:68	lignosulphonate bio-waste	44:68	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	5	50	with	membranes	1031:1039	arg1	content					1061:1067	the highest LS content	1046:1067	the highest LS content	1046:1067	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	5	50	with	membranes	1031:1039	arg1	resistance					1097:1106	high electronic resistance	1081:1106	high electronic resistance	1081:1106	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	4	51	theme	final	834:838	arg1	membranes					840:848	the final membranes	830:848	the final membranes	830:848	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	3	52	theme	CNF	620:622	arg1	aggregation					605:615	the aggregation	601:615	the aggregation of CNF	601:622	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	1	53	theme	biomass	139:145	arg1	objective					191:199	a core objective	184:199	a core objective	184:199	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	1	53	theme	biomass	139:145	arg1	use					132:134	The use	128:134	The use of biomass to produce value-adding materials	128:179	The use of biomass to produce value-adding materials is a core objective of the circular economy, which has attracted great research interest in recent decades.					
33142658	4	54	contain	has	776:778	arg2	effect					791:796	an adverse effect	780:796	an adverse effect on the mechanical properties of the final membranes	780:848	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	4	54	contain	has	776:778	arg1	Addition					724:731	Addition	724:731	Addition of both the LS and CaCl2 to CNF dispersion	724:774	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	0	55	theme	flame	85:89	arg1	retardant					91:99	excellent flame retardant	75:99	excellent flame retardant	75:99	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	5	56	theme	%	981:981	arg1	capacity					1001:1008	100 % UVA/UVB shielding capacity	977:1008	100 % UVA/UVB shielding capacity	977:1008	The effectiveness of the new composite membranes has been described in terms of their passive (charring) flame retardancy and 100 % UVA/UVB shielding capacity, both identified for membranes with the highest LS content, as well as high electronic resistance.					
33142658	3	57	theme	electrostatic	561:573	arg1	screening					575:583	an electrostatic screening	558:583	an electrostatic screening	558:583	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	3	58	theme	CaCl2	494:498	arg1	addition					482:489	The addition	478:489	The addition of CaCl2 to CNF and LS dispersions	478:524	The addition of CaCl2 to CNF and LS dispersions reduces the ζ potential, due to an electrostatic screening, which promotes the aggregation of CNF, increases its moisture content and promotes LS deposition on CNFs already in the dispersion phase.					
33142658	4	59	dep	LS	745:746	arg1	the					741:743	the	741:743	the	741:743	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
33142658	0	60	theme	excellent	75:83	arg1	retardant					91:99	excellent flame retardant	75:99	excellent flame retardant	75:99	Consolidation of cellulose nanofibrils with lignosulphonate bio-waste into excellent flame retardant and UV blocking membranes.					
33142658	4	61	theme	LS	745:746	arg1	Addition					724:731	Addition	724:731	Addition of both the LS and CaCl2 to CNF dispersion	724:774	Addition of both the LS and CaCl2 to CNF dispersion has an adverse effect on the mechanical properties of the final membranes.					
32196027	0	0	theme	efficient	89:97	arg1	uranium					99:105	highly efficient uranium	82:105	highly efficient uranium	82:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	2	1	theme	hydraulic/ionic	440:454	arg1	conductivity					456:467	good hydraulic/ionic conductivity	435:467	good hydraulic/ionic conductivity	435:467	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	1	2	theme	cellulose	149:157	arg1	hydrogel					191:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel is explored via UV-polymerization.					
32196027	2	3	contain	has	249:251	arg1	hydrogel					240:247	This hydrogel	235:247	This hydrogel	235:247	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	3	contain	has	249:251	arg2	capacity					288:295	a highly efficient uranium capture capacity	253:295	a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	253:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	4	theme	good	435:438	arg1	conductivity					456:467	good hydraulic/ionic conductivity	435:467	good hydraulic/ionic conductivity	435:467	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	1	5	theme	paper-poly	159:168	arg1	hydrogel					191:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel is explored via UV-polymerization.					
32196027	2	6	theme	12.9	338:341	arg1	mg					343:344	mg	343:344	mg	343:344	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	7	theme	up	300:301	arg1	capacity					288:295	a highly efficient uranium capture capacity	253:295	a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	253:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	8	theme	mg	343:344	arg1	g-1					346:348	12.9 mg g-1	338:348	12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	338:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	9	theme	antibacterial	488:500	arg1	performance					502:512	broad-spectrum antibacterial performance	473:512	broad-spectrum antibacterial performance	473:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	10	theme	capture	280:286	arg1	capacity					288:295	a highly efficient uranium capture capacity	253:295	a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	253:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	11	theme	broad-spectrum	473:486	arg1	performance					502:512	broad-spectrum antibacterial performance	473:512	broad-spectrum antibacterial performance	473:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	12	theme	uranium	272:278	arg1	capacity					288:295	a highly efficient uranium capture capacity	253:295	a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	253:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	1	13	theme	amidoxime	170:178	arg1	hydrogel					191:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel is explored via UV-polymerization.					
32196027	0	14	theme	antibacterial	13:25	arg1	hydrogel					69:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel	0:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium	0:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	2	15	theme	mg	311:312	arg1	g-1					314:316	6.21 mg g-1	306:316	6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	306:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	0	16	theme	Bio-inspired	0:11	arg1	hydrogel					69:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel	0:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium	0:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	2	17	theme	6.21	306:309	arg1	g-1					314:316	6.21 mg g-1	306:316	6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	306:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	1	18	theme	composite	181:189	arg1	hydrogel					191:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel is explored via UV-polymerization.					
32196027	0	19	theme	paper-poly	37:46	arg1	hydrogel					69:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel	0:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium	0:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	0	20	theme	cellulose	27:35	arg1	hydrogel					69:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel	0:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium	0:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	2	21	theme	due	399:401	arg1	weeks					392:396	6 weeks	390:396	6 weeks	390:396	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	0	22	dep	capture	111:117	arg1	vi					107:108	vi	107:108	vi	107:108	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	0	23	theme	amidoxime	48:56	arg1	hydrogel					69:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel	0:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium	0:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	0	24	theme	composite	59:67	arg1	hydrogel					69:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel	0:76	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium	0:105	Bio-inspired antibacterial cellulose paper-poly(amidoxime) composite hydrogel for highly efficient uranium(vi) capture from seawater.					
32196027	2	25	from	WU/Wpoly	354:361	arg1	seawater					377:384	seawater	377:384	seawater	377:384	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	26	theme	efficient	262:270	arg1	capacity					288:295	a highly efficient uranium capture capacity	253:295	a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance	253:512	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	2	27	theme	enhanced	410:417	arg1	hydrophilicity					419:432	its enhanced hydrophilicity	406:432	its enhanced hydrophilicity	406:432	This hydrogel has a highly efficient uranium capture capacity of up to 6.21 mg g-1 for WU/Wdry gel and 12.9 mg g-1 for WU/Wpoly(amidoxime) in seawater for 6 weeks, due to its enhanced hydrophilicity, good hydraulic/ionic conductivity and broad-spectrum antibacterial performance.					
32196027	1	28	theme	bio-inspired	136:147	arg1	hydrogel					191:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel	134:198	A bio-inspired cellulose paper-poly(amidoxime) composite hydrogel is explored via UV-polymerization.					
33993857	6	0	theme	FA	736:737	arg1	composition					739:749	FA composition	736:749	FA composition	736:749	FA composition was analyzed by gas chromatography, and FA ratios were used to estimate the activity of the enzymes.					
33993857	7	1	theme	Fads1	899:903	arg1	higher					925:930	higher	925:930	higher	925:930	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	1	theme	Fads1	899:903	arg1	levels					873:878	the levels	869:878	the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs	869:918	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	2	theme	Elovl6	891:896	arg1	higher					925:930	higher	925:930	higher	925:930	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	2	theme	Elovl6	891:896	arg1	levels					873:878	the levels	869:878	the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs	869:918	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	3	theme	Elovl5	883:888	arg1	higher					925:930	higher	925:930	higher	925:930	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	3	theme	Elovl5	883:888	arg1	levels					873:878	the levels	869:878	the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs	869:918	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	12	4	theme	dietary	1648:1654	arg1	status					1656:1661	the dietary status	1644:1661	the dietary status	1644:1661	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	9	5	from	increase	1211:1218	arg1	level					1227:1231	the level	1223:1231	the level of Scd mRNA	1223:1243	In contrast, an increase in the level of Scd mRNA was accompanied by a decrease in the enzyme activity.					
33993857	8	6	theme	Higher	995:1000	arg1	levels					1002:1007	Higher levels	995:1007	Higher levels of Elovl5 and Elovl6 mRNAs	995:1034	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	4	7	dep	diet	643:646	arg1	ST					649:650	ST	649:650	ST	649:650	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	4	8	theme	Male	551:554	arg1	rats					563:566	Male Wistar rats	551:566	Male Wistar rats	551:566	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	7	9	theme	HSD	859:861	arg1	rats					863:866	the HSD rats	855:866	the HSD rats	855:866	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	1	10	from	tissue	172:177	arg1	present					149:155	present	149:155	present	149:155	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	4	11	theme	Wistar	556:561	arg1	rats					563:566	Male Wistar rats	551:566	Male Wistar rats	551:566	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	1	12	mod	modified	191:198	arg3	desaturases					217:227	desaturases	217:227	desaturases	217:227	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	12	mod	modified	191:198	arg1	FAs					144:146	FAs	144:146	FAs	144:146	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	12	mod	modified	191:198	arg1	acids					137:141	Fatty acids	131:141	Fatty acids (FAs) present in the adipose tissue (AT)	131:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	12	mod	modified	191:198	arg3	elongases					203:211	elongases	203:211	elongases	203:211	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	13	from	present	149:155	arg1	AT					180:181	AT	180:181	AT	180:181	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	13	from	present	149:155	arg1	tissue					172:177	the adipose tissue	160:177	the adipose tissue (AT)	160:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	9	14	from	decrease	1266:1273	arg1	activity					1289:1296	the enzyme activity	1278:1296	the enzyme activity	1278:1296	In contrast, an increase in the level of Scd mRNA was accompanied by a decrease in the enzyme activity.					
33993857	1	15	theme	Fatty	131:135	arg1	FAs					144:146	FAs	144:146	FAs	144:146	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	15	theme	Fatty	131:135	arg1	acids					137:141	Fatty acids	131:141	Fatty acids (FAs) present in the adipose tissue (AT)	131:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	8	16	theme	relative	1059:1066	arg1	activities					1068:1077	higher relative activities	1052:1077	higher relative activities of these enzymes	1052:1094	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	3	17	dep	desaturases	454:464	arg1	Scd					481:483	Scd	481:483	Scd	481:483	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	17	dep	desaturases	454:464	arg1	Fads2					474:478	Fads2	474:478	Fads2	474:478	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	17	dep	desaturases	454:464	arg1	Fads1					467:471	Fads1	467:471	Fads1	467:471	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	0	18	from	Impact	0:5	arg1	Levels					40:45	the mRNA Levels	31:45	the mRNA Levels for Elongases and Desaturases	31:75	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	0	18	from	Impact	0:5	arg1	Activity					99:106	Estimated Protein Activity	81:106	Estimated Protein Activity in Rat Adipose Tissue	81:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	7	19	theme	Fads2	952:956	arg1	mRNA					958:961	Fads2 mRNA	952:961	Fads2 mRNA	952:961	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	12	20	theme	AT	1597:1598	arg1	composition					1606:1616	the AT lipid composition	1593:1616	the AT lipid composition in response to changes in the dietary status	1593:1661	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	8	21	theme	enzymes	1088:1094	arg1	activities					1068:1077	higher relative activities	1052:1077	higher relative activities of these enzymes	1052:1094	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	10	22	theme	HSD	1351:1353	arg1	rats					1355:1358	HSD rats	1351:1358	HSD rats	1351:1358	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	0	23	theme	Protein	91:97	arg1	Activity					99:106	Estimated Protein Activity	81:106	Estimated Protein Activity in Rat Adipose Tissue	81:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	3	24	theme	diet	364:367	arg1	impact					341:346	the impact	337:346	the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT	337:548	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	25	theme	study	315:319	arg1	aim					304:306	The aim	300:306	The aim of the study	300:319	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	4	26	theme	study	593:597	arg1	groups					599:604	two study groups	589:604	two study groups: fed with an HSD and with a standard diet (ST)	589:651	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	4	27	theme	standard	634:641	arg1	diet					643:646	a standard diet	632:646	a standard diet (ST)	632:651	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	0	28	theme	High-Sucrose	10:21	arg1	Diet					23:26	High-Sucrose Diet	10:26	High-Sucrose Diet	10:26	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	12	29	theme	elongases	1532:1540	arg1	activity					1515:1522	activity	1515:1522	activity of both elongases and desaturases	1515:1556	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	12	29	theme	elongases	1532:1540	arg1	regulation					1485:1494	the regulation	1481:1494	the regulation of mRNA levels	1481:1509	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	9	30	theme	enzyme	1282:1287	arg1	activity					1289:1296	the enzyme activity	1278:1296	the enzyme activity	1278:1296	In contrast, an increase in the level of Scd mRNA was accompanied by a decrease in the enzyme activity.					
33993857	8	31	theme	Fads2	1125:1129	arg1	mRNA					1131:1134	the Fads2 mRNA	1121:1134	the Fads2 mRNA	1121:1134	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	3	32	theme	68	375:376	arg1	%					377:377	%	377:377	%	377:377	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	7	33	theme	mRNAs	914:918	arg1	higher					925:930	higher	925:930	higher	925:930	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	33	theme	mRNAs	914:918	arg1	levels					873:878	the levels	869:878	the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs	869:918	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	12	34	theme	desaturases	1546:1556	arg1	activity					1515:1522	activity	1515:1522	activity of both elongases and desaturases	1515:1556	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	12	34	theme	desaturases	1546:1556	arg1	regulation					1485:1494	the regulation	1481:1494	the regulation of mRNA levels	1481:1509	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	1	35	attach	present	149:155	arg2	FAs					144:146	FAs	144:146	FAs	144:146	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	35	attach	present	149:155	arg1	AT					180:181	AT	180:181	AT	180:181	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	35	attach	present	149:155	arg2	acids					137:141	Fatty acids	131:141	Fatty acids (FAs) present in the adipose tissue (AT)	131:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	35	attach	present	149:155	arg1	tissue					172:177	the adipose tissue	160:177	the adipose tissue (AT)	160:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	0	36	theme	mRNA	35:38	arg1	Levels					40:45	the mRNA Levels	31:45	the mRNA Levels for Elongases and Desaturases	31:75	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	12	37	theme	levels	1504:1509	arg1	activity					1515:1522	activity	1515:1522	activity of both elongases and desaturases	1515:1556	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	12	37	theme	levels	1504:1509	arg1	regulation					1485:1494	the regulation	1481:1494	the regulation of mRNA levels	1481:1509	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	4	38	dep	groups	599:604	arg1	fed					607:609	fed	607:609	fed	607:609	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	11	39	theme	FAs	1412:1414	arg1	composition					1386:1396	The composition	1382:1396	The composition of individual FAs	1382:1414	The composition of individual FAs differed between the groups.					
33993857	3	40	theme	%	377:377	arg1	sucrose					379:385	68% sucrose	375:385	68% sucrose	375:385	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	8	41	theme	lower	1160:1164	arg1	activity					1166:1173	the lower activity	1156:1173	the lower activity of this desaturase	1156:1192	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	8	42	dep	Elovl5	1012:1017	arg1	mRNAs					1030:1034	mRNAs	1030:1034	mRNAs	1030:1034	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	3	43	theme	mRNAs	405:409	arg1	levels					395:400	the levels	391:400	the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd)	391:484	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	44	theme	rat	543:545	arg1	AT					547:548	the rat AT	539:548	the rat AT	539:548	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	6	45	used	used	806:809	arg2	ratios					794:799	FA ratios	791:799	FA ratios	791:799	FA composition was analyzed by gas chromatography, and FA ratios were used to estimate the activity of the enzymes.					
33993857	9	46	theme	Scd	1236:1238	arg1	mRNA					1240:1243	Scd mRNA	1236:1243	Scd mRNA	1236:1243	In contrast, an increase in the level of Scd mRNA was accompanied by a decrease in the enzyme activity.					
33993857	3	47	from	activity	497:504	arg1	AT					547:548	the rat AT	539:548	the rat AT	539:548	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	5	48	theme	mRNA	658:661	arg1	levels					663:668	The mRNA levels	654:668	The mRNA levels	654:668	The mRNA levels were determined by a semi-quantitative reverse transcription-PCR.					
33993857	10	49	theme	monounsaturated	1304:1318	arg1	FAs					1320:1322	Less monounsaturated FAs	1299:1322	Less monounsaturated FAs	1299:1322	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	2	50	theme	different	261:269	arg1	nutrients					289:297	nutrients	289:297	nutrients	289:297	These enzymes are regulated by different factors including nutrients.					
33993857	2	50	theme	different	261:269	arg1	factors					271:277	different factors	261:277	different factors including nutrients	261:297	These enzymes are regulated by different factors including nutrients.					
33993857	1	51	theme	present	149:155	arg1	FAs					144:146	FAs	144:146	FAs	144:146	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	51	theme	present	149:155	arg1	acids					137:141	Fatty acids	131:141	Fatty acids (FAs) present in the adipose tissue (AT)	131:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	6	52	theme	FA	791:792	arg1	ratios					794:799	FA ratios	791:799	FA ratios	791:799	FA composition was analyzed by gas chromatography, and FA ratios were used to estimate the activity of the enzymes.					
33993857	3	53	theme	proteins	527:534	arg1	activity					497:504	the activity	493:504	the activity of the corresponding proteins in the rat AT	493:548	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	4	54	with	fed	607:609	arg1	diet					643:646	a standard diet	632:646	a standard diet (ST)	632:651	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	4	54	with	fed	607:609	arg1	HSD					619:621	an HSD	616:621	an HSD	616:621	Male Wistar rats were randomized into two study groups: fed with an HSD and with a standard diet (ST).					
33993857	0	55	theme	Rat	111:113	arg1	Tissue					123:128	Rat Adipose Tissue	111:128	Rat Adipose Tissue	111:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	5	56	theme	reverse	709:715	arg1	transcription-PCR					717:733	a semi-quantitative reverse transcription-PCR	689:733	a semi-quantitative reverse transcription-PCR	689:733	The mRNA levels were determined by a semi-quantitative reverse transcription-PCR.					
33993857	9	57	theme	mRNA	1240:1243	arg1	level					1227:1231	the level	1223:1231	the level of Scd mRNA	1223:1243	In contrast, an increase in the level of Scd mRNA was accompanied by a decrease in the enzyme activity.					
33993857	6	58	theme	gas	767:769	arg1	chromatography					771:784	gas chromatography	767:784	gas chromatography	767:784	FA composition was analyzed by gas chromatography, and FA ratios were used to estimate the activity of the enzymes.					
33993857	8	59	theme	desaturase	1183:1192	arg1	activity					1166:1173	the lower activity	1156:1173	the lower activity of this desaturase	1156:1192	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	0	60	from	Activity	99:106	arg1	Tissue					123:128	Rat Adipose Tissue	111:128	Rat Adipose Tissue	111:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	8	61	theme	Elovl5	1012:1017	arg1	levels					1002:1007	Higher levels	995:1007	Higher levels of Elovl5 and Elovl6 mRNAs	995:1034	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	8	62	theme	Elovl6	1023:1028	arg1	levels					1002:1007	Higher levels	995:1007	Higher levels of Elovl5 and Elovl6 mRNAs	995:1034	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	7	63	theme	mRNA	958:961	arg1	lower					967:971	lower	967:971	lower	967:971	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	7	63	theme	mRNA	958:961	arg1	level					943:947	the level	939:947	the level of Fads2 mRNA	939:961	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	3	64	from	impact	341:346	arg1	levels					395:400	the levels	391:400	the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd)	391:484	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	64	from	impact	341:346	arg1	activity					497:504	the activity	493:504	the activity of the corresponding proteins in the rat AT	493:548	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	5	65	theme	semi-quantitative	691:707	arg1	transcription-PCR					717:733	a semi-quantitative reverse transcription-PCR	689:733	a semi-quantitative reverse transcription-PCR	689:733	The mRNA levels were determined by a semi-quantitative reverse transcription-PCR.					
33993857	8	66	theme	higher	1052:1057	arg1	activities					1068:1077	higher relative activities	1052:1077	higher relative activities of these enzymes	1052:1094	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	0	67	theme	Estimated	81:89	arg1	Activity					99:106	Estimated Protein Activity	81:106	Estimated Protein Activity in Rat Adipose Tissue	81:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	12	68	theme	lipid	1600:1604	arg1	composition					1606:1616	the AT lipid composition	1593:1616	the AT lipid composition in response to changes in the dietary status	1593:1661	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	0	69	from	Levels	40:45	arg1	Tissue					123:128	Rat Adipose Tissue	111:128	Rat Adipose Tissue	111:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	12	70	theme	important	1566:1574	arg1	role					1576:1579	an important role	1563:1579	an important role	1563:1579	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	1	71	theme	adipose	164:170	arg1	AT					180:181	AT	180:181	AT	180:181	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	1	71	theme	adipose	164:170	arg1	tissue					172:177	the adipose tissue	160:177	the adipose tissue (AT)	160:182	Fatty acids (FAs) present in the adipose tissue (AT) can be modified by elongases and desaturases.					
33993857	3	72	theme	corresponding	513:525	arg1	proteins					527:534	the corresponding proteins	509:534	the corresponding proteins	509:534	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	0	73	theme	Diet	23:26	arg1	Impact					0:5	Impact	0:5	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.	0:129	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	3	74	dep	elongases	415:423	arg1	Elovl5					434:439	Elovl5	434:439	Elovl5	434:439	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	74	dep	elongases	415:423	arg1	Elovl2					426:431	Elovl2	426:431	Elovl2	426:431	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	74	dep	elongases	415:423	arg1	Elovl6					442:447	Elovl6	442:447	Elovl6	442:447	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	7	75	theme	Scd	910:912	arg1	mRNAs					914:918	Scd mRNAs	910:918	Scd mRNAs	910:918	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
33993857	6	76	theme	enzymes	843:849	arg1	activity					827:834	the activity	823:834	the activity of the enzymes	823:849	FA composition was analyzed by gas chromatography, and FA ratios were used to estimate the activity of the enzymes.					
33993857	3	77	theme	high-sucrose	351:362	arg1	HSD					370:372	HSD	370:372	HSD	370:372	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	3	77	theme	high-sucrose	351:362	arg1	diet					364:367	high-sucrose diet	351:367	high-sucrose diet (HSD; 68% sucrose)	351:386	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	10	78	theme	rats	1355:1358	arg1	AT					1345:1346	the AT	1341:1346	the AT of HSD rats	1341:1358	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	12	79	from	changes	1633:1639	arg1	status					1656:1661	the dietary status	1644:1661	the dietary status	1644:1661	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	3	80	dep	HSD	370:372	arg1	sucrose					379:385	68% sucrose	375:385	68% sucrose	375:385	The aim of the study was to evaluate the impact of high-sucrose diet (HSD; 68% sucrose) on the levels of mRNAs for elongases (Elovl2, Elovl5, Elovl6) and desaturases (Fads1, Fads2, Scd) and on the activity of the corresponding proteins in the rat AT.					
33993857	0	81	theme	Adipose	115:121	arg1	Tissue					123:128	Rat Adipose Tissue	111:128	Rat Adipose Tissue	111:128	Impact of High-Sucrose Diet on the mRNA Levels for Elongases and Desaturases and Estimated Protein Activity in Rat Adipose Tissue.					
33993857	11	82	theme	individual	1401:1410	arg1	FAs					1412:1414	individual FAs	1401:1414	individual FAs	1401:1414	The composition of individual FAs differed between the groups.					
33993857	8	83	theme	mRNA	1131:1134	arg1	downregulation					1103:1116	downregulation	1103:1116	downregulation of the Fads2 mRNA	1103:1134	Higher levels of Elovl5 and Elovl6 mRNAs corresponded to higher relative activities of these enzymes, while downregulation of the Fads2 mRNA was associated with the lower activity of this desaturase.					
33993857	12	84	theme	mRNA	1499:1502	arg1	levels					1504:1509	mRNA levels	1499:1509	mRNA levels	1499:1509	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	10	85	located	detected	1329:1336	arg1	AT					1345:1346	the AT	1341:1346	the AT of HSD rats	1341:1358	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	10	85	located	detected	1329:1336	arg1	group					1375:1379	the ST group	1368:1379	the ST group	1368:1379	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	10	85	located	detected	1329:1336	arg2	FAs					1320:1322	Less monounsaturated FAs	1299:1322	Less monounsaturated FAs	1299:1322	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	10	86	theme	ST	1372:1373	arg1	group					1375:1379	the ST group	1368:1379	the ST group	1368:1379	Less monounsaturated FAs were detected in the AT of HSD rats than in the ST group.					
33993857	12	87	from	composition	1606:1616	arg1	response					1621:1628	response	1621:1628	response to changes in the dietary status	1621:1661	This study supports the notion that the regulation of mRNA levels and activity of both elongases and desaturases play an important role in managing the AT lipid composition in response to changes in the dietary status.					
33993857	7	88	theme	ST	985:986	arg1	group					988:992	the ST group	981:992	the ST group	981:992	In the HSD rats, the levels of Elovl5, Elovl6, Fads1, and Scd mRNAs were higher, while the level of Fads2 mRNA was lower than in the ST group.					
31958562	1	0	theme	FCF	275:277	arg1	removal					222:228	efficient removal	212:228	efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution	212:314	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	7	1	theme	DR	986:987	arg1	model					989:993	DR model	986:993	DR model for Sy and Bb	986:1007	Adsorption energies obtained from DR model for Sy and Bb showed that adsorption processes were chemically.					
31958562	3	2	theme	adsorbent	403:411	arg1	properties					413:422	The adsorbent properties	399:422	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes	399:465	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	2	3	theme	PZC	385:387	arg1	analysis					389:396	PZC analysis	385:396	PZC analysis	385:396	Ch-V composite beads were characterized by using FTIR, SEM, XRD and PZC analysis.					
31958562	1	4	theme	composite	119:127	arg1	material					135:142	Chitosan (Ch)-Vermiculite (V) composite beads material	89:142	Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent	89:196	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	4	theme	composite	119:127	arg1	adsorbent					188:196	adsorbent	188:196	adsorbent	188:196	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	5	theme	efficient	212:220	arg1	removal					222:228	efficient removal	212:228	efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution	212:314	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	4	6	theme	Freundlich	636:645	arg1	models					686:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	The experimental data presented were obtained from Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models.					
31958562	6	7	theme	experimental	859:870	arg1	data					872:875	the experimental data	855:875	the experimental data	855:875	The results showed that the experimental data were better fit the Langmiur model for Sy and the Freundlich model for Bb.					
31958562	5	8	theme	Langmuir	734:741	arg1	equation					743:750	the Langmuir equation	730:750	the Langmuir equation	730:750	The maximum adsorption capacity for the Langmuir equation was found to be 0.387 mol kg-1 for Sy and 0.229 mol kg-1 for Bb, respectively.					
31958562	1	9	theme	food	284:287	arg1	dyes					289:292	food dyes	284:292	food dyes	284:292	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	7	10	theme	Adsorption	952:961	arg1	energies					963:970	Adsorption energies	952:970	Adsorption energies obtained from DR model for Sy and Bb	952:1007	Adsorption energies obtained from DR model for Sy and Bb showed that adsorption processes were chemically.					
31958562	3	11	theme	composite	432:440	arg1	beads					442:446	Ch-V composite beads	427:446	Ch-V composite beads	427:446	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	8	12	theme	adsorption	1122:1131	arg1	kinetics					1133:1140	adsorption kinetics	1122:1140	adsorption kinetics	1122:1140	The pseudo-second order and intra-particle diffusion models of adsorption kinetics are in accordance with Sy and Bb.					
31958562	1	13	theme	beads	129:133	arg1	material					135:142	Chitosan (Ch)-Vermiculite (V) composite beads material	89:142	Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent	89:196	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	13	theme	beads	129:133	arg1	adsorbent					188:196	adsorbent	188:196	adsorbent	188:196	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	14	from	solution	307:314	arg1	removal					222:228	efficient removal	212:228	efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution	212:314	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	9	15	dep	Sy	1219:1220	arg1	adsorption					1229:1238	adsorption	1229:1238	adsorption	1229:1238	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	4	16	theme	isotherm	677:684	arg1	models					686:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	The experimental data presented were obtained from Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models.					
31958562	3	17	theme	beads	442:446	arg1	properties					413:422	The adsorbent properties	399:422	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes	399:465	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	8	18	theme	diffusion	1102:1110	arg1	models					1112:1117	The pseudo-second order and intra-particle diffusion models	1059:1117	models	1112:1117	The pseudo-second order and intra-particle diffusion models of adsorption kinetics are in accordance with Sy and Bb.					
31958562	7	19	theme	adsorption	1021:1030	arg1	processes					1032:1040	adsorption processes	1021:1040	adsorption processes	1021:1040	Adsorption energies obtained from DR model for Sy and Bb showed that adsorption processes were chemically.					
31958562	6	20	theme	Freundlich	927:936	arg1	model					938:942	the Freundlich model	923:942	the Freundlich model for Bb	923:949	The results showed that the experimental data were better fit the Langmiur model for Sy and the Freundlich model for Bb.					
31958562	1	21	theme	Sunset	233:238	arg1	FCF					247:249	Sunset Yellow FCF	233:249	Sunset Yellow FCF (Sy)	233:254	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	21	theme	Sunset	233:238	arg1	Sy					252:253	Sy	252:253	Sy	252:253	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	22	theme	Yellow	240:245	arg1	FCF					247:249	Sunset Yellow FCF	233:249	Sunset Yellow FCF (Sy)	233:254	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	22	theme	Yellow	240:245	arg1	Sy					252:253	Sy	252:253	Sy	252:253	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	4	23	theme	Langmuir	626:633	arg1	models					686:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	The experimental data presented were obtained from Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models.					
31958562	5	24	theme	adsorption	706:715	arg1	0.387 mol kg-1					768:781	0.387 mol kg-1	768:781	0.387 mol kg-1	768:781	The maximum adsorption capacity for the Langmuir equation was found to be 0.387 mol kg-1 for Sy and 0.229 mol kg-1 for Bb, respectively.					
31958562	5	24	theme	adsorption	706:715	arg1	capacity					717:724	The maximum adsorption capacity	694:724	The maximum adsorption capacity for the Langmuir equation	694:750	The maximum adsorption capacity for the Langmuir equation was found to be 0.387 mol kg-1 for Sy and 0.229 mol kg-1 for Bb, respectively.					
31958562	0	25	theme	food	11:14	arg1	Removal					0:6	Removal	0:6	Removal of food	0:14	Removal of food dyes from aqueous solution by chitosan-vermiculite beads.					
31958562	1	26	theme	FCF	247:249	arg1	removal					222:228	efficient removal	212:228	efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution	212:314	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	4	27	theme	Dubinin-Radushkevich	651:670	arg1	models					686:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models	626:691	The experimental data presented were obtained from Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models.					
31958562	1	28	theme	aqueous	299:305	arg1	solution					307:314	aqueous solution	299:314	aqueous solution	299:314	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	8	29	theme	kinetics	1133:1140	arg1	order					1077:1081	The pseudo-second order and intra-particle diffusion models	1059:1117	order	1077:1081	The pseudo-second order and intra-particle diffusion models of adsorption kinetics are in accordance with Sy and Bb.					
31958562	8	29	theme	kinetics	1133:1140	arg1	models					1112:1117	The pseudo-second order and intra-particle diffusion models	1059:1117	models	1112:1117	The pseudo-second order and intra-particle diffusion models of adsorption kinetics are in accordance with Sy and Bb.					
31958562	0	30	theme	aqueous	26:32	arg1	solution					34:41	aqueous solution	26:41	aqueous solution	26:41	Removal of food dyes from aqueous solution by chitosan-vermiculite beads.					
31958562	9	31	theme	evaluation	1205:1214	arg1	it					1241:1242	it	1241:1242	it	1241:1242	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	9	31	theme	evaluation	1205:1214	arg1	result					1181:1186	a result	1179:1186	a result of thermodynamic evaluation of Sy and Bb adsorption	1179:1238	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	2	32	theme	Ch-V	317:320	arg1	beads					332:336	Ch-V composite beads	317:336	Ch-V composite beads	317:336	Ch-V composite beads were characterized by using FTIR, SEM, XRD and PZC analysis.					
31958562	9	33	theme	Sy	1219:1220	arg1	evaluation					1205:1214	thermodynamic evaluation	1191:1214	thermodynamic evaluation of Sy and Bb adsorption	1191:1238	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	8	34	theme	pseudo-second	1063:1075	arg1	order					1077:1081	The pseudo-second order and intra-particle diffusion models	1059:1117	order	1077:1081	The pseudo-second order and intra-particle diffusion models of adsorption kinetics are in accordance with Sy and Bb.					
31958562	3	35	theme	kinetic	513:519	arg1	time					522:525	kinetic (time) and thermodynamic (temperature)	513:558	time	522:525	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	3	35	theme	kinetic	513:519	arg1	pH					494:495	pH	494:495	pH	494:495	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	2	36	theme	composite	322:330	arg1	beads					332:336	Ch-V composite beads	317:336	Ch-V composite beads	317:336	Ch-V composite beads were characterized by using FTIR, SEM, XRD and PZC analysis.					
31958562	8	37	theme	intra-particle	1087:1100	arg1	models					1112:1117	The pseudo-second order and intra-particle diffusion models	1059:1117	models	1112:1117	The pseudo-second order and intra-particle diffusion models of adsorption kinetics are in accordance with Sy and Bb.					
31958562	9	38	theme	thermodynamic	1191:1203	arg1	evaluation					1205:1214	thermodynamic evaluation	1191:1214	thermodynamic evaluation of Sy and Bb adsorption	1191:1238	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	4	39	theme	experimental	579:590	arg1	data					592:595	The experimental data	575:595	The experimental data presented	575:605	The experimental data presented were obtained from Langmuir, Freundlich and Dubinin-Radushkevich (DR) isotherm models.					
31958562	1	40	dep	FCF	247:249	arg1	dyes					289:292	food dyes	284:292	food dyes	284:292	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	9	41	theme	Bb	1226:1227	arg1	evaluation					1205:1214	thermodynamic evaluation	1191:1214	thermodynamic evaluation of Sy and Bb adsorption	1191:1238	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	1	42	theme	Blue	270:273	arg1	Bb					280:281	Bb	280:281	Bb	280:281	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	42	theme	Blue	270:273	arg1	FCF					275:277	Brilliant Blue FCF	260:277	Brilliant Blue FCF (Bb)	260:282	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	43	used	used	203:206	arg2	adsorbent					188:196	adsorbent	188:196	adsorbent	188:196	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	43	used	used	203:206	arg2	material					135:142	Chitosan (Ch)-Vermiculite (V) composite beads material	89:142	Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent	89:196	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	44	theme	Brilliant	260:268	arg1	Bb					280:281	Bb	280:281	Bb	280:281	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	1	44	theme	Brilliant	260:268	arg1	FCF					275:277	Brilliant Blue FCF	260:277	Brilliant Blue FCF (Bb)	260:282	In this study, Chitosan (Ch)-Vermiculite (V) composite beads material which is a low-cost and naturally effective adsorbent were used for efficient removal of Sunset Yellow FCF (Sy) and Brilliant Blue FCF (Bb) food dyes from aqueous solution.					
31958562	6	45	theme	Langmiur	897:904	arg1	model					906:910	the Langmiur model	893:910	the Langmiur model for Sy	893:917	The results showed that the experimental data were better fit the Langmiur model for Sy and the Freundlich model for Bb.					
31958562	9	46	theme	adsorption	1259:1268	arg1	processes					1270:1278	adsorption processes	1259:1278	adsorption processes	1259:1278	As a result of thermodynamic evaluation of Sy and Bb adsorption, it was found that adsorption processes were endothermic, entropy increased and reaction was spontaneous.					
31958562	3	47	dep	Sy	452:453	arg1	dyes					462:465	dyes	462:465	dyes	462:465	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	3	48	theme	thermodynamic	532:544	arg1	pH					494:495	pH	494:495	pH	494:495	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	3	48	theme	thermodynamic	532:544	arg1	temperature					547:557	kinetic (time) and thermodynamic (temperature)	513:558	temperature	547:557	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	3	49	theme	pH	494:495	arg1	terms					485:489	terms	485:489	terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption	485:572	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	0	50	theme	chitosan-vermiculite	46:65	arg1	beads					67:71	chitosan-vermiculite beads	46:71	chitosan-vermiculite beads	46:71	Removal of food dyes from aqueous solution by chitosan-vermiculite beads.					
31958562	3	51	theme	adsorption	563:572	arg1	terms					485:489	terms	485:489	terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption	485:572	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
31958562	5	52	theme	maximum	698:704	arg1	0.387 mol kg-1					768:781	0.387 mol kg-1	768:781	0.387 mol kg-1	768:781	The maximum adsorption capacity for the Langmuir equation was found to be 0.387 mol kg-1 for Sy and 0.229 mol kg-1 for Bb, respectively.					
31958562	5	52	theme	maximum	698:704	arg1	capacity					717:724	The maximum adsorption capacity	694:724	The maximum adsorption capacity for the Langmuir equation	694:750	The maximum adsorption capacity for the Langmuir equation was found to be 0.387 mol kg-1 for Sy and 0.229 mol kg-1 for Bb, respectively.					
31958562	3	53	theme	Ch-V	427:430	arg1	beads					442:446	Ch-V composite beads	427:446	Ch-V composite beads	427:446	The adsorbent properties of Ch-V composite beads for Sy and Bb dyes were evaluated in terms of pH, concentration, kinetic (time) and thermodynamic (temperature) of adsorption.					
34081956	4	0	theme	significant	799:809	arg1	acceleration					823:834	significant coagulation acceleration	799:834	significant coagulation acceleration	799:834	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	1	1	theme	military	144:151	arg1	trauma					153:158	military trauma	144:158	military trauma	144:158	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	0	2	theme	rapid	72:76	arg1	hemostasis					78:87	rapid hemostasis	72:87	rapid hemostasis	72:87	A composite sponge based on alkylated chitosan and diatom-biosilica for rapid hemostasis.					
34081956	2	3	theme	composite	305:313	arg1	sponge					315:320	a safe and effective hemostatic composite sponge	273:320	a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control	273:358	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	3	theme	composite	305:313	arg1	sponge					329:334	AC-DB sponge	323:334	AC-DB sponge	323:334	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	6	4	theme	great	1019:1023	arg1	potential					1025:1033	great potential	1019:1033	great potential	1019:1033	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	3	5	theme	favorable	538:546	arg1	biocompatibility					548:563	favorable biocompatibility	538:563	favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity)	538:603	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	4	6	theme	erythrocyte	677:687	arg1	deformation					715:725	deformation	715:725	deformation	715:725	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	6	theme	erythrocyte	677:687	arg1	activation					703:712	the erythrocyte and platelets activation	673:712	the erythrocyte and platelets activation	673:712	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	6	theme	erythrocyte	677:687	arg1	activation					774:783	intrinsic coagulation pathway activation	744:783	intrinsic coagulation pathway activation	744:783	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	6	theme	erythrocyte	677:687	arg1	aggregation					731:741	aggregation	731:741	aggregation	731:741	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	6	7	theme	rapid	1052:1056	arg1	material					1069:1076	a safe and rapid hemostatic material	1041:1076	a safe and rapid hemostatic material	1041:1076	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	5	8	theme	clotting	913:920	arg1	time					922:925	shortest clotting time	904:925	shortest clotting time (106.2 s)	904:935	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	8	theme	clotting	913:920	arg1	106.2 s					928:934	106.2 s	928:934	106.2 s	928:934	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	4	9	theme	coagulation	811:821	arg1	acceleration					823:834	significant coagulation acceleration	799:834	significant coagulation acceleration	799:834	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	1	10	from	significance	128:139	arg1	accidents					172:180	traffic accidents	164:180	traffic accidents	164:180	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	1	10	from	significance	128:139	arg1	trauma					153:158	military trauma	144:158	military trauma	144:158	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	4	11	theme	strong	610:615	arg1	effect					627:632	The strong interface effect	606:632	The strong interface effect between AC-DB sponge and blood	606:663	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	2	12	theme	safe	275:278	arg1	sponge					315:320	a safe and effective hemostatic composite sponge	273:320	a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control	273:358	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	12	theme	safe	275:278	arg1	sponge					329:334	AC-DB sponge	323:334	AC-DB sponge	323:334	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	13	theme	hemostatic	294:303	arg1	sponge					315:320	a safe and effective hemostatic composite sponge	273:320	a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control	273:358	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	13	theme	hemostatic	294:303	arg1	sponge					329:334	AC-DB sponge	323:334	AC-DB sponge	323:334	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	3	14	theme	clotting	469:476	arg1	time					478:481	clotting time	469:481	clotting time was shortened by 78% than that of control group	469:529	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	2	15	theme	hemorrhage	341:350	arg1	control					352:358	hemorrhage control	341:358	hemorrhage control	341:358	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	4	16	theme	pathway	766:772	arg1	activation					703:712	the erythrocyte and platelets activation	673:712	the erythrocyte and platelets activation	673:712	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	16	theme	pathway	766:772	arg1	activation					774:783	intrinsic coagulation pathway activation	744:783	intrinsic coagulation pathway activation	744:783	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	3	17	theme	rapid	434:438	arg1	ability					451:457	rapid hemostatic ability	434:457	rapid hemostatic ability	434:457	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	1	18	theme	traffic	164:170	arg1	accidents					172:180	traffic accidents	164:180	traffic accidents	164:180	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	0	19	theme	composite	2:10	arg1	sponge					12:17	A composite sponge	0:17	A composite sponge	0:17	A composite sponge based on alkylated chitosan and diatom-biosilica for rapid hemostasis.					
34081956	1	20	theme	Rapid	90:94	arg1	control					96:102	Rapid control	90:102	Rapid control of bleeding	90:114	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	3	21	theme	procoagulant	372:383	arg1	structure					394:402	the procoagulant chemical structure	368:402	the procoagulant chemical structure of AC-DB sponge	368:418	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	4	22	theme	intrinsic	744:752	arg1	activation					703:712	the erythrocyte and platelets activation	673:712	the erythrocyte and platelets activation	673:712	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	22	theme	intrinsic	744:752	arg1	activation					774:783	intrinsic coagulation pathway activation	744:783	intrinsic coagulation pathway activation	744:783	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	6	23	theme	hemostatic	1058:1067	arg1	material					1069:1076	a safe and rapid hemostatic material	1041:1076	a safe and rapid hemostatic material	1041:1076	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	3	24	theme	sponge	413:418	arg1	structure					394:402	the procoagulant chemical structure	368:402	the procoagulant chemical structure of AC-DB sponge	368:418	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	4	25	theme	platelets	693:701	arg1	deformation					715:725	deformation	715:725	deformation	715:725	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	25	theme	platelets	693:701	arg1	activation					703:712	the erythrocyte and platelets activation	673:712	the erythrocyte and platelets activation	673:712	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	25	theme	platelets	693:701	arg1	activation					774:783	intrinsic coagulation pathway activation	744:783	intrinsic coagulation pathway activation	744:783	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	25	theme	platelets	693:701	arg1	aggregation					731:741	aggregation	731:741	aggregation	731:741	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	3	26	theme	hemolysis	566:574	arg1	%					585:585	hemolysis ratio < 5%	566:585	hemolysis ratio < 5%	566:585	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	3	26	theme	hemolysis	566:574	arg1	cytotoxicity					591:602	no cytotoxicity	588:602	no cytotoxicity	588:602	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	5	27	theme	shortest	904:911	arg1	time					922:925	shortest clotting time	904:925	shortest clotting time (106.2 s)	904:935	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	27	theme	shortest	904:911	arg1	106.2 s					928:934	106.2 s	928:934	106.2 s	928:934	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	6	28	theme	above	976:980	arg1	results					982:988	All above results	972:988	All above results	972:988	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	0	29	theme	alkylated	28:36	arg1	chitosan					38:45	chitosan	38:45	chitosan	38:45	A composite sponge based on alkylated chitosan and diatom-biosilica for rapid hemostasis.					
34081956	3	30	theme	hemostatic	440:449	arg1	ability					451:457	rapid hemostatic ability	434:457	rapid hemostatic ability	434:457	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	6	31	theme	safe	1043:1046	arg1	material					1069:1076	a safe and rapid hemostatic material	1041:1076	a safe and rapid hemostatic material	1041:1076	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	3	32	theme	control	517:523	arg1	group					525:529	control group	517:529	control group	517:529	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	3	33	theme	chemical	385:392	arg1	structure					394:402	the procoagulant chemical structure	368:402	the procoagulant chemical structure of AC-DB sponge	368:418	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	3	34	theme	AC-DB	407:411	arg1	sponge					413:418	AC-DB sponge	407:418	AC-DB sponge	407:418	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	4	35	theme	coagulation	754:764	arg1	activation					703:712	the erythrocyte and platelets activation	673:712	the erythrocyte and platelets activation	673:712	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	4	35	theme	coagulation	754:764	arg1	activation					774:783	intrinsic coagulation pathway activation	744:783	intrinsic coagulation pathway activation	744:783	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	1	36	theme	bleeding	107:114	arg1	control					96:102	Rapid control	90:102	Rapid control of bleeding	90:114	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	6	37	contain	had	1015:1017	arg2	potential					1025:1033	great potential	1019:1033	great potential	1019:1033	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	6	37	contain	had	1015:1017	arg1	sponge					1008:1013	AC-DB sponge	1002:1013	AC-DB sponge	1002:1013	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	2	38	theme	alkylated	198:206	arg1	AC					218:219	AC	218:219	AC	218:219	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	38	theme	alkylated	198:206	arg1	chitosan					208:215	alkylated chitosan	198:215	alkylated chitosan (AC)	198:220	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	39	theme	effective	284:292	arg1	sponge					315:320	a safe and effective hemostatic composite sponge	273:320	a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control	273:358	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	39	theme	effective	284:292	arg1	sponge					329:334	AC-DB sponge	323:334	AC-DB sponge	323:334	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	5	40	from	performance	864:874	arg1	assessments					887:897	in vivo assessments	879:897	in vivo assessments	879:897	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	4	41	theme	AC-DB	642:646	arg1	sponge					648:653	AC-DB sponge	642:653	AC-DB sponge	642:653	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
34081956	3	42	dep	biocompatibility	548:563	arg1	%					585:585	hemolysis ratio < 5%	566:585	hemolysis ratio < 5%	566:585	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	3	42	dep	biocompatibility	548:563	arg1	cytotoxicity					591:602	no cytotoxicity	588:602	no cytotoxicity	588:602	Due to the procoagulant chemical structure of AC-DB sponge, it exhibited rapid hemostatic ability in vitro (clotting time was shortened by 78% than that of control group), with favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity).					
34081956	5	43	theme	excellent	854:862	arg1	performance					864:874	excellent performance	854:874	excellent performance in in vivo assessments	854:897	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	2	44	theme	diatom	226:231	arg1	DB					244:245	DB	244:245	DB	244:245	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	44	theme	diatom	226:231	arg1	biosilica					233:241	diatom biosilica	226:241	diatom biosilica (DB)	226:246	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	5	45	contain	had	850:852	arg1	sponge					843:848	AC-DB sponge	837:848	AC-DB sponge	837:848	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	45	contain	had	850:852	arg2	performance					864:874	excellent performance	854:874	excellent performance in in vivo assessments	854:897	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	1	46	theme	great	122:126	arg1	significance					128:139	great significance	122:139	great significance	122:139	Rapid control of bleeding is of great significance in military trauma and traffic accidents.					
34081956	5	47	dep	in	879:880	arg1	vivo					882:885	vivo	882:885	vivo	882:885	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	48	theme	in	879:880	arg1	assessments					887:897	in vivo assessments	879:897	in vivo assessments	879:897	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	49	theme	minimal	941:947	arg1	328.5 mg					961:968	328.5 mg	961:968	328.5 mg	961:968	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	49	theme	minimal	941:947	arg1	loss					955:958	minimal blood loss	941:958	minimal blood loss (328.5 mg)	941:969	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	2	50	theme	AC-DB	323:327	arg1	sponge					315:320	a safe and effective hemostatic composite sponge	273:320	a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control	273:358	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	2	50	theme	AC-DB	323:327	arg1	sponge					329:334	AC-DB sponge	323:334	AC-DB sponge	323:334	In this study, alkylated chitosan (AC) and diatom biosilica (DB) were combined to develop a safe and effective hemostatic composite sponge (AC-DB sponge) for hemorrhage control.					
34081956	6	51	theme	AC-DB	1002:1006	arg1	sponge					1008:1013	AC-DB sponge	1002:1013	AC-DB sponge	1002:1013	All above results proved that AC-DB sponge had great potential to be a safe and rapid hemostatic material.					
34081956	5	52	theme	blood	949:953	arg1	328.5 mg					961:968	328.5 mg	961:968	328.5 mg	961:968	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	52	theme	blood	949:953	arg1	loss					955:958	minimal blood loss	941:958	minimal blood loss (328.5 mg)	941:969	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	5	53	theme	AC-DB	837:841	arg1	sponge					843:848	AC-DB sponge	837:848	AC-DB sponge	837:848	AC-DB sponge had excellent performance in in vivo assessments with shortest clotting time (106.2 s) and minimal blood loss (328.5 mg).					
34081956	4	54	theme	interface	617:625	arg1	effect					627:632	The strong interface effect	606:632	The strong interface effect between AC-DB sponge and blood	606:663	The strong interface effect between AC-DB sponge and blood induced the erythrocyte and platelets activation, deformation and aggregation, intrinsic coagulation pathway activation, resulting in significant coagulation acceleration.					
32049968	3	0	theme	cognitive	616:624	arg1	development					626:636	infant cognitive development	609:636	infant cognitive development	609:636	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	7	1	theme	development	1038:1048	arg1	score					1050:1054	Infant cognitive development score	1021:1054	Infant cognitive development score	1021:1054	Infant cognitive development score was assessed with the Bayley-III Scale at 24 months.					
32049968	1	2	theme	early	255:259	arg1	feeding					261:267	early feeding	255:267	early feeding	255:267	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	2	3	from	role	323:326	arg1	memory					408:413	memory	408:413	memory	408:413	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	3	from	role	323:326	arg1	learning					395:402	learning	395:402	learning	395:402	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	14	4	theme	infant	1989:1994	arg1	development					2006:2016	infant cognitive development	1989:2016	infant cognitive development through greater exposure to 2'FL relative to other HMOs	1989:2072	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	3	5	theme	age	654:656	arg1	months					644:649	24 months	641:649	24 months of age	641:656	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	7	6	theme	Infant	1021:1026	arg1	score					1050:1054	Infant cognitive development score	1021:1054	Infant cognitive development score	1021:1054	Infant cognitive development score was assessed with the Bayley-III Scale at 24 months.					
32049968	6	7	from	interest	1003:1010	arg1	FL					1017:1018	2'FL	1015:1018	2'FL	1015:1018	Nineteen HMOs were analyzed using high-performance liquid chromatography, with initial interest in 2'FL.					
32049968	4	8	theme	=	776:776	arg1	N					774:774	N = 50	774:779	N = 50	774:779	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	4	8	theme	=	776:776	arg1	pairs					767:771	Hispanic mother-infant pairs	744:771	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50)	722:780	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	1	9	theme	cognitive	172:180	arg1	development					182:192	BACKGROUND Infant cognitive development	154:192	BACKGROUND Infant cognitive development	154:192	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	0	10	theme	overweight	134:143	arg1	infants					112:118	infants	112:118	infants of normal and overweight mothers	112:151	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	11	11	theme	mediation	1607:1615	arg1	effect					1617:1622	estimated mediation effect	1597:1622	estimated mediation effect = 0.13	1597:1629	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	10	12	dep	predicted	1408:1416	arg1	P≤					1460:1461	P≤	1460:1461	P≤	1460:1461	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	8	13	theme	Linear	1109:1114	arg1	regressions					1116:1126	Linear regressions	1109:1126	Linear regressions	1109:1126	Linear regressions were used for prediction, and bootstrapping to determine mediation by 2'FL.					
32049968	15	14	dep	FL	2166:2167	arg1	2					2164:2164	2	2164:2164	2	2164:2164	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	2	15	theme	other	506:510	arg1	factors					530:536	other HMOs and maternal factors	506:536	other HMOs and maternal factors	506:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	16	theme	Animal	298:303	arg1	studies					305:311	Animal studies	298:311	Animal studies	298:311	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	17	theme	relative	494:501	arg1	development					482:492	infant cognitive development	465:492	infant cognitive development relative to other HMOs and maternal factors	465:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	14	18	theme	breast	1943:1948	arg1	milk					1950:1953	breast milk	1943:1953	breast milk feeding at 1 month	1943:1972	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	3	19	theme	feeding	703:709	arg1	frequency					711:719	feeding frequency	703:719	feeding frequency	703:719	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	0	20	theme	normal	123:128	arg1	infants					112:118	infants	112:118	infants of normal and overweight mothers	112:151	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	12	21	theme	cognitive	1719:1727	arg1	development					1729:1739	infant cognitive development	1712:1739	infant cognitive development	1712:1739	There were no associations of feedings/day and 2'FL at 6 months with infant cognitive development.					
32049968	8	22	used	used	1133:1136	arg2	regressions					1116:1126	Linear regressions	1109:1126	Linear regressions	1109:1126	Linear regressions were used for prediction, and bootstrapping to determine mediation by 2'FL.					
32049968	0	23	from	month	65:69	arg1	feedings					51:58	feedings	51:58	feedings at 1 month	51:69	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	10	24	theme	infant	1425:1430	arg1	development					1442:1452	better infant cognitive development	1418:1452	better infant cognitive development	1418:1452	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	3	25	theme	breast	691:696	arg1	milk					698:701	breast milk	691:701	breast milk	691:701	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	1	26	from	obesity	244:250	arg1	range					233:237	range	233:237	range from obesity to early feeding and breast milk composition	233:295	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	0	27	from	months	102:107	arg1	infants					112:118	infants	112:118	infants of normal and overweight mothers	112:151	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	5	28	theme	Breast	839:844	arg1	milk					846:849	Breast milk	839:849	Breast milk	839:849	Breast milk was collected at 1 and 6 months, and feedings/day were reported.					
32049968	13	29	dep	CONCLUSIONS	1742:1752	arg1	suggest					1767:1773	suggest	1767:1773	suggest that maternal factors influence infant cognitive development through multiple means	1767:1857	CONCLUSIONS Our findings suggest that maternal factors influence infant cognitive development through multiple means.					
32049968	4	30	theme	BMI	834:836	arg1	spectrum					808:815	the spectrum	804:815	the spectrum of pre-pregnancy BMI	804:836	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	2	31	from	impact	455:460	arg1	development					482:492	infant cognitive development	465:492	infant cognitive development relative to other HMOs and maternal factors	465:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	11	32	theme	further	1568:1574	arg1	adjustment					1576:1585	further adjustment	1568:1585	further adjustment for 2'FL	1568:1594	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	15	33	theme	temporal	2187:2194	arg1	window					2196:2201	a critical temporal window	2176:2201	a critical temporal window for positively influencing infant cognitive development	2176:2257	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	15	33	theme	temporal	2187:2194	arg1	exposure					2152:2159	early exposure	2146:2159	early exposure to 2'FL	2146:2167	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	11	34	theme	=	1624:1624	arg1	effect					1617:1622	estimated mediation effect	1597:1622	estimated mediation effect = 0.13	1597:1629	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	7	35	theme	Bayley-III	1078:1087	arg1	Scale					1089:1093	the Bayley-III Scale	1074:1093	the Bayley-III Scale	1074:1093	Infant cognitive development score was assessed with the Bayley-III Scale at 24 months.					
32049968	0	36	dep	normal	123:128	arg1	mothers					145:151	mothers	145:151	mothers	145:151	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	14	37	theme	greater	1922:1928	arg1	frequency					1930:1938	greater frequency	1922:1938	greater frequency of breast milk feeding at 1 month	1922:1972	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	2	38	theme	HMOs	512:515	arg1	factors					530:536	other HMOs and maternal factors	506:536	other HMOs and maternal factors	506:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	15	39	theme	early	2146:2150	arg1	window					2196:2201	a critical temporal window	2176:2201	a critical temporal window for positively influencing infant cognitive development	2176:2257	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	15	39	theme	early	2146:2150	arg1	exposure					2152:2159	early exposure	2146:2159	early exposure to 2'FL	2146:2167	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	0	40	theme	cognitive	74:82	arg1	development					84:94	cognitive development	74:94	cognitive development	74:94	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	3	41	dep	obesity	679:685	arg1	frequency					711:719	feeding frequency	703:719	feeding frequency	703:719	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	1	42	theme	BACKGROUND	154:163	arg1	development					182:192	BACKGROUND Infant cognitive development	154:192	BACKGROUND Infant cognitive development	154:192	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	2	43	theme	cognitive	472:480	arg1	development					482:492	infant cognitive development	465:492	infant cognitive development relative to other HMOs and maternal factors	465:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	10	44	dep	Feedings/day	1353:1364	arg1	β					1367:1367	β	1367:1367	β = 0.34	1367:1374	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	9	45	theme	RESULTS	1204:1210	arg1	BMI					1235:1237	RESULTS Maternal pre-pregnancy BMI	1204:1237	RESULTS Maternal pre-pregnancy BMI	1204:1237	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	14	46	theme	separate	1893:1900	arg1	influence					1911:1919	a separate negative influence	1891:1919	a separate negative influence	1891:1919	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	14	46	theme	separate	1893:1900	arg1	obesity					1876:1882	maternal obesity	1867:1882	maternal obesity	1867:1882	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	14	47	theme	other	2063:2067	arg1	HMOs					2069:2072	other HMOs	2063:2072	other HMOs	2063:2072	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	9	48	theme	pre-pregnancy	1221:1233	arg1	BMI					1235:1237	RESULTS Maternal pre-pregnancy BMI	1204:1237	RESULTS Maternal pre-pregnancy BMI	1204:1237	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	6	49	theme	initial	995:1001	arg1	interest					1003:1010	initial interest	995:1010	initial interest in 2'FL	995:1018	Nineteen HMOs were analyzed using high-performance liquid chromatography, with initial interest in 2'FL.					
32049968	0	50	theme	Human	0:4	arg1	2'-fucosyllactose					27:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	1	51	theme	breast	273:278	arg1	composition					285:295	breast milk composition	273:295	breast milk composition	273:295	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	15	52	theme	cognitive	2237:2245	arg1	development					2247:2257	infant cognitive development	2230:2257	infant cognitive development	2230:2257	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	3	53	theme	breast	586:591	arg1	milk					593:596	breast milk	586:596	breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency	586:719	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	10	54	theme	=	1369:1369	arg1	β					1367:1367	β	1367:1367	β = 0.34	1367:1374	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	0	55	theme	oligosaccharide	11:25	arg1	2'-fucosyllactose					27:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	6	56	theme	liquid	967:972	arg1	chromatography					974:987	high-performance liquid chromatography	950:987	high-performance liquid chromatography	950:987	Nineteen HMOs were analyzed using high-performance liquid chromatography, with initial interest in 2'FL.					
32049968	2	57	theme	human	423:427	arg1	studies					429:435	no human studies	420:435	no human studies	420:435	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	11	58	theme	cognitive	1514:1522	arg1	development					1524:1534	infant cognitive development	1507:1534	infant cognitive development	1507:1534	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	4	59	theme	mother-infant	753:765	arg1	N					774:774	N = 50	774:779	N = 50	774:779	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	4	59	theme	mother-infant	753:765	arg1	pairs					767:771	Hispanic mother-infant pairs	744:771	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50)	722:780	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	9	60	theme	=	1333:1333	arg1	β					1331:1331	β	1331:1331	β = -0.31	1331:1339	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	3	61	theme	FL	578:579	arg1	impact					566:571	the impact	562:571	the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency	562:719	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	9	62	dep	=	1333:1333	arg1	-0.31					1335:1339	-0.31	1335:1339	-0.31	1335:1339	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	2	63	theme	milk	338:341	arg1	HMO					360:362	HMO	360:362	HMO	360:362	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	63	theme	milk	338:341	arg1	2'-fucosyllactose					366:382	2'-fucosyllactose	366:382	2'-fucosyllactose (2'FL)	366:389	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	63	theme	milk	338:341	arg1	oligosaccharide					343:357	human milk oligosaccharide	332:357	human milk oligosaccharide (HMO)	332:363	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	3	64	theme	infant	609:614	arg1	development					626:636	infant cognitive development	609:636	infant cognitive development	609:636	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	10	65	dep	P≤	1460:1461	arg1	both					1455:1458	both	1455:1458	both	1455:1458	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	13	66	theme	cognitive	1814:1822	arg1	development					1824:1834	infant cognitive development	1807:1834	infant cognitive development	1807:1834	CONCLUSIONS Our findings suggest that maternal factors influence infant cognitive development through multiple means.					
32049968	15	67	theme	FL	2094:2095	arg1	influence					2079:2087	The influence	2075:2087	The influence of 2'FL	2075:2095	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	15	67	theme	FL	2094:2095	arg1	significant					2105:2115	significant	2105:2115	significant	2105:2115	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	3	68	from	milk	593:596	arg1	impact					566:571	the impact	562:571	the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency	562:719	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	9	69	dep	=	1344:1344	arg1	β					1331:1331	β	1331:1331	β = -0.31	1331:1339	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	9	70	theme	poorer	1294:1299	arg1	development					1318:1328	poorer infant cognitive development	1294:1328	poorer infant cognitive development	1294:1328	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	10	71	from	month	1402:1406	arg1	Feedings/day					1353:1364	Feedings/day	1353:1364	Feedings/day (β = 0.34)	1353:1375	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	10	71	from	month	1402:1406	arg1	FL					1383:1384	2'FL	1381:1384	2'FL (β = 0.59) at 1 month	1381:1406	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	7	72	theme	cognitive	1028:1036	arg1	score					1050:1054	Infant cognitive development score	1021:1054	Infant cognitive development score	1021:1054	Infant cognitive development score was assessed with the Bayley-III Scale at 24 months.					
32049968	13	73	theme	maternal	1780:1787	arg1	factors					1789:1795	maternal factors	1780:1795	maternal factors	1780:1795	CONCLUSIONS Our findings suggest that maternal factors influence infant cognitive development through multiple means.					
32049968	9	74	theme	cognitive	1308:1316	arg1	development					1318:1328	poorer infant cognitive development	1294:1328	poorer infant cognitive development	1294:1328	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	14	75	theme	cognitive	1996:2004	arg1	development					2006:2016	infant cognitive development	1989:2016	infant cognitive development through greater exposure to 2'FL relative to other HMOs	1989:2072	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	1	76	theme	Infant	165:170	arg1	development					182:192	BACKGROUND Infant cognitive development	154:192	BACKGROUND Infant cognitive development	154:192	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	0	77	attach	links	45:49	arg1	development					84:94	cognitive development	74:94	cognitive development	74:94	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	0	77	attach	links	45:49	arg2	2'-fucosyllactose					27:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	14	78	dep	FL	2048:2049	arg1	2					2046:2046	2	2046:2046	2	2046:2046	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	10	79	theme	better	1418:1423	arg1	development					1442:1452	better infant cognitive development	1418:1452	better infant cognitive development	1418:1452	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	4	80	dep	METHODS	722:728	arg1	N					774:774	N = 50	774:779	N = 50	774:779	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	4	80	dep	METHODS	722:728	arg1	pairs					767:771	Hispanic mother-infant pairs	744:771	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50)	722:780	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	14	81	theme	milk	1950:1953	arg1	frequency					1930:1938	greater frequency	1922:1938	greater frequency of breast milk feeding at 1 month	1922:1972	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	11	82	theme	estimated	1597:1605	arg1	effect					1617:1622	estimated mediation effect	1597:1622	estimated mediation effect = 0.13	1597:1629	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	1	83	theme	maternal	211:218	arg1	factors					220:226	maternal factors	211:226	maternal factors that range from obesity to early feeding and breast milk composition	211:295	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	10	84	theme	cognitive	1432:1440	arg1	development					1442:1452	better infant cognitive development	1418:1452	better infant cognitive development	1418:1452	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	15	85	from	months	2122:2127	arg1	influence					2079:2087	The influence	2075:2087	The influence of 2'FL	2075:2095	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	15	85	from	months	2122:2127	arg1	significant					2105:2115	significant	2105:2115	significant	2105:2115	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	10	86	dep	FL	1383:1384	arg1	=					1389:1389	=	1389:1389	=	1389:1389	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	15	87	theme	infant	2230:2235	arg1	development					2247:2257	infant cognitive development	2230:2257	infant cognitive development	2230:2257	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	12	88	theme	infant	1712:1717	arg1	development					1729:1739	infant cognitive development	1712:1739	infant cognitive development	1712:1739	There were no associations of feedings/day and 2'FL at 6 months with infant cognitive development.					
32049968	2	89	theme	maternal	521:528	arg1	factors					530:536	other HMOs and maternal factors	506:536	other HMOs and maternal factors	506:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	4	90	theme	pre-pregnancy	820:832	arg1	BMI					834:836	pre-pregnancy BMI	820:836	pre-pregnancy BMI	820:836	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	12	91	theme	FL	1692:1693	arg1	associations					1657:1668	no associations	1654:1668	no associations of feedings/day and 2'FL	1654:1693	There were no associations of feedings/day and 2'FL at 6 months with infant cognitive development.					
32049968	15	92	theme	critical	2178:2185	arg1	window					2196:2201	a critical temporal window	2176:2201	a critical temporal window for positively influencing infant cognitive development	2176:2257	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	15	92	theme	critical	2178:2185	arg1	exposure					2152:2159	early exposure	2146:2159	early exposure to 2'FL	2146:2167	The influence of 2'FL was not significant at 6 months, indicating that early exposure to 2'FL may be a critical temporal window for positively influencing infant cognitive development.					
32049968	11	93	dep	=	1624:1624	arg1	0.13					1626:1629	0.13	1626:1629	0.13	1626:1629	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	11	94	dep	=	1634:1634	arg1	effect					1617:1622	estimated mediation effect	1597:1622	estimated mediation effect = 0.13	1597:1629	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	2	95	theme	infant	465:470	arg1	development					482:492	infant cognitive development	465:492	infant cognitive development relative to other HMOs and maternal factors	465:536	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	14	96	theme	negative	1902:1909	arg1	influence					1911:1919	a separate negative influence	1891:1919	a separate negative influence	1891:1919	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	14	96	theme	negative	1902:1909	arg1	obesity					1876:1882	maternal obesity	1867:1882	maternal obesity	1867:1882	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	10	97	dep	=	1369:1369	arg1	0.34					1371:1374	0.34	1371:1374	0.34	1371:1374	Feedings/day (β = 0.34) and 2'FL (β = 0.59) at 1 month predicted better infant cognitive development (both P≤ 0.01).					
32049968	9	98	theme	Maternal	1212:1219	arg1	BMI					1235:1237	RESULTS Maternal pre-pregnancy BMI	1204:1237	RESULTS Maternal pre-pregnancy BMI	1204:1237	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
32049968	12	99	theme	feedings/day	1673:1684	arg1	associations					1657:1668	no associations	1654:1668	no associations of feedings/day and 2'FL	1654:1693	There were no associations of feedings/day and 2'FL at 6 months with infant cognitive development.					
32049968	0	100	theme	milk	6:9	arg1	2'-fucosyllactose					27:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose	0:43	Human milk oligosaccharide 2'-fucosyllactose links feedings at 1 month to cognitive development at 24 months in infants of normal and overweight mothers.					
32049968	3	101	theme	maternal	670:677	arg1	obesity					679:685	maternal obesity	670:685	maternal obesity	670:685	OBJECTIVE To determine the impact of 2'FL from breast milk feeding on infant cognitive development at 24 months of age relative to maternal obesity and breast milk feeding frequency.					
32049968	1	102	theme	milk	280:283	arg1	composition					285:295	breast milk composition	273:295	breast milk composition	273:295	BACKGROUND Infant cognitive development is influenced by maternal factors that range from obesity to early feeding and breast milk composition.					
32049968	14	103	theme	maternal	1867:1874	arg1	influence					1911:1919	a separate negative influence	1891:1919	a separate negative influence	1891:1919	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	14	103	theme	maternal	1867:1874	arg1	obesity					1876:1882	maternal obesity	1867:1882	maternal obesity	1867:1882	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	11	104	theme	infant	1507:1512	arg1	development					1524:1534	infant cognitive development	1507:1534	infant cognitive development	1507:1534	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	6	105	theme	high-performance	950:965	arg1	chromatography					974:987	high-performance liquid chromatography	950:987	high-performance liquid chromatography	950:987	Nineteen HMOs were analyzed using high-performance liquid chromatography, with initial interest in 2'FL.					
32049968	11	106	theme	feedings/day	1489:1500	arg1	significant					1550:1560	significant	1550:1560	significant	1550:1560	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	11	106	theme	feedings/day	1489:1500	arg1	association					1474:1484	The association	1470:1484	The association of feedings/day with infant cognitive development	1470:1534	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	4	107	theme	Hispanic	744:751	arg1	N					774:774	N = 50	774:779	N = 50	774:779	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	4	107	theme	Hispanic	744:751	arg1	pairs					767:771	Hispanic mother-infant pairs	744:771	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50)	722:780	METHODS AND MATERIALS Hispanic mother-infant pairs (N = 50) were recruited across the spectrum of pre-pregnancy BMI.					
32049968	13	108	theme	multiple	1844:1851	arg1	means					1853:1857	multiple means	1844:1857	multiple means	1844:1857	CONCLUSIONS Our findings suggest that maternal factors influence infant cognitive development through multiple means.					
32049968	13	109	theme	infant	1807:1812	arg1	development					1824:1834	infant cognitive development	1807:1834	infant cognitive development	1807:1834	CONCLUSIONS Our findings suggest that maternal factors influence infant cognitive development through multiple means.					
32049968	2	110	theme	human	332:336	arg1	HMO					360:362	HMO	360:362	HMO	360:362	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	110	theme	human	332:336	arg1	2'-fucosyllactose					366:382	2'-fucosyllactose	366:382	2'-fucosyllactose (2'FL)	366:389	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	2	110	theme	human	332:336	arg1	oligosaccharide					343:357	human milk oligosaccharide	332:357	human milk oligosaccharide (HMO)	332:363	Animal studies suggest a role for human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), on learning and memory, yet no human studies have examined its impact on infant cognitive development relative to other HMOs and maternal factors.					
32049968	11	111	with	association	1474:1484	arg1	development					1524:1534	infant cognitive development	1507:1534	infant cognitive development	1507:1534	The association of feedings/day with infant cognitive development was no longer significant after further adjustment for 2'FL (estimated mediation effect = 0.13, P = 0.04).					
32049968	14	112	theme	greater	2026:2032	arg1	exposure					2034:2041	greater exposure	2026:2041	greater exposure to 2'FL relative to other HMOs	2026:2072	Though maternal obesity may be a separate negative influence, greater frequency of breast milk feeding at 1 month contributed to infant cognitive development through greater exposure to 2'FL relative to other HMOs.					
32049968	9	113	theme	infant	1301:1306	arg1	development					1318:1328	poorer infant cognitive development	1294:1328	poorer infant cognitive development	1294:1328	RESULTS Maternal pre-pregnancy BMI was not related to feedings/day or HMOs, but predicted poorer infant cognitive development (β = -0.31, P = 0.03).					
34617943	7	0	theme	15.64	1280:1284	arg1	%					1285:1285	%	1285:1285	%	1285:1285	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	7	1	theme	acid	1186:1189	arg1	rate					1205:1208	the free fatty acid (FFA) release rate	1171:1208	the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase	1171:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	1	2	theme	small	261:265	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	9	3	theme	lipolysis	1650:1658	arg1	purpose					1611:1617	the purpose	1607:1617	the purpose of enhanced stability, delayed lipolysis or sustained nutrient release	1607:1688	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	7	4	theme	free	1175:1178	arg1	FFA					1192:1194	FFA	1192:1194	FFA	1192:1194	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	7	4	theme	free	1175:1178	arg1	acid					1186:1189	free fatty acid	1175:1189	the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase	1171:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	1	5	theme	soft	268:271	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	9	6	theme	sustained	1663:1671	arg1	release					1682:1688	sustained nutrient release	1663:1688	sustained nutrient release	1663:1688	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	1	7	used	used	163:166	arg2	we					160:161	we	160:161	we	160:161	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	7	8	theme	emulsions	1223:1231	arg1	rate					1205:1208	the free fatty acid (FFA) release rate	1171:1208	the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase	1171:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	9	9	theme	release	1682:1688	arg1	purpose					1611:1617	the purpose	1607:1617	the purpose of enhanced stability, delayed lipolysis or sustained nutrient release	1607:1688	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	7	10	theme	complex	1152:1158	arg1	interface					1160:1168	the complex interface	1148:1168	the complex interface	1148:1168	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	1	11	theme	hydrophilic	186:196	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	11	theme	hydrophilic	186:196	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	3	12	theme	oil/water	609:617	arg1	interface					619:627	the oil/water interface	605:627	the oil/water interface	605:627	Microstructural observations showed that ZPCPs were effectively adsorbed at the oil/water interface despite the substantial interparticle gaps.					
34617943	4	13	theme	WPM	673:675	arg1	particles					677:685	WPM particles	673:685	WPM particles	673:685	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	1	14	theme	glycol	213:218	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	14	theme	glycol	213:218	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	15	theme	composite	229:237	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	15	theme	composite	229:237	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	8	16	theme	Pickering	1414:1422	arg1	emulsion					1424:1431	the Pickering emulsion	1410:1431	the Pickering emulsion	1410:1431	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	4	17	theme	deformable	741:750	arg1	structure					752:760	their deformable structure	735:760	their deformable structure	735:760	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	8	18	used	used	1313:1316	arg2	ZPCPs					1302:1306	ZPCPs	1302:1306	ZPCPs	1302:1306	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	18	used	used	1313:1316	arg2	WPM					1341:1343	WPM	1341:1343	WPM	1341:1343	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	18	used	used	1313:1316	arg2	layer					1331:1335	the inner layer	1321:1335	the inner layer	1321:1335	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	18	used	used	1313:1316	arg2	layer					1358:1362	the outer layer	1348:1362	the outer layer	1348:1362	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	5	19	theme	mass	962:965	arg1	ratio					967:971	mass ratio	962:971	mass ratio	962:971	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	8	20	theme	best	1444:1447	arg1	stability					1449:1457	stability	1449:1457	stability	1449:1457	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	3	21	theme	substantial	641:651	arg1	gaps					667:670	the substantial interparticle gaps	637:670	the substantial interparticle gaps	637:670	Microstructural observations showed that ZPCPs were effectively adsorbed at the oil/water interface despite the substantial interparticle gaps.					
34617943	9	22	theme	Pickering	1496:1504	arg1	emulsion					1506:1513	The Pickering emulsion	1492:1513	The Pickering emulsion with particle-particle complex interfaces	1492:1555	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	2	23	theme	combined	492:499	arg1	use					501:503	the combined use	488:503	the combined use of different particles	488:526	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	9	24	with	emulsion	1506:1513	arg1	interfaces					1546:1555	particle-particle complex interfaces	1520:1555	particle-particle complex interfaces	1520:1555	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	0	25	theme	particle-particle	107:123	arg1	interfaces					133:142	particle-particle complex interfaces	107:142	particle-particle complex interfaces	107:142	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	9	26	theme	complex	1538:1544	arg1	interfaces					1546:1555	particle-particle complex interfaces	1520:1555	particle-particle complex interfaces	1520:1555	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	2	27	theme	particles	518:526	arg1	use					501:503	the combined use	488:503	the combined use of different particles	488:526	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	4	28	theme	particles	813:821	arg1	layer					794:798	an interfacial layer	779:798	an interfacial layer of flattened particles to cover a large surface area	779:851	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	1	29	theme	Pickering	359:367	arg1	emulsion					369:376	a Pickering emulsion	357:376	a Pickering emulsion for delivery of β-carotene	357:403	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	0	30	theme	Enhanced	0:7	arg1	stability					9:17	Enhanced stability	0:17	Enhanced stability	0:17	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	7	31	dep	%	1285:1285	arg1	to					1287:1288	to	1287:1288	to	1287:1288	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	2	32	theme	storage	437:443	arg1	stability					445:453	storage stability	437:453	storage stability	437:453	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	4	33	theme	interfacial	782:792	arg1	layer					794:798	an interfacial layer	779:798	an interfacial layer of flattened particles to cover a large surface area	779:851	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	1	34	theme	hydrophobic	278:288	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	5	35	theme	different	998:1006	arg1	particles					1008:1016	different particles	998:1016	different particles	998:1016	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	8	36	theme	ZPCPs	1386:1390	arg1	 1					1406:1407	 1	1406:1407	 1	1406:1407	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	36	theme	ZPCPs	1386:1390	arg1	ratio					1377:1381	the mass ratio	1368:1381	the mass ratio of ZPCPs to WPM	1368:1397	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	0	37	theme	gastrointestinal	34:49	arg1	digestion					51:59	controlled gastrointestinal digestion	23:59	controlled gastrointestinal digestion	23:59	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	1	38	theme	protein	295:301	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	7	39	theme	release	1197:1203	arg1	rate					1205:1208	the free fatty acid (FFA) release rate	1171:1208	the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase	1171:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	6	40	theme	gastrointestinal	1081:1096	arg1	digestion					1098:1106	gastrointestinal digestion	1081:1106	gastrointestinal digestion	1081:1106	The combination of ZPCPs and WPM delayed the lipolysis during gastrointestinal digestion.					
34617943	2	41	dep	stability	423:431	arg1	The					406:408	The	406:408	The	406:408	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	4	42	theme	large	834:838	arg1	area					848:851	a large surface area	832:851	a large surface area	832:851	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	9	43	theme	stability	1631:1639	arg1	purpose					1611:1617	the purpose	1607:1617	the purpose of enhanced stability, delayed lipolysis or sustained nutrient release	1607:1688	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	7	44	theme	9.03	1290:1293	arg1	%					1285:1285	%	1285:1285	%	1285:1285	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	7	45	theme	fatty	1180:1184	arg1	FFA					1192:1194	FFA	1192:1194	FFA	1192:1194	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	7	45	theme	fatty	1180:1184	arg1	acid					1186:1189	free fatty acid	1175:1189	the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase	1171:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	9	46	theme	delayed	1642:1648	arg1	lipolysis					1650:1658	delayed lipolysis	1642:1658	delayed lipolysis	1642:1658	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	7	47	theme	interface	1160:1168	arg1	composition					1133:1143	the composition	1129:1143	the composition of the complex interface	1129:1168	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	4	48	from	stretch	703:709	arg1	interface					718:726	the interface	714:726	the interface due to their deformable structure	714:760	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	1	49	theme	rigid	175:179	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	49	theme	rigid	175:179	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	9	50	theme	nutrient	1673:1680	arg1	release					1682:1688	sustained nutrient release	1663:1688	sustained nutrient release	1663:1688	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	4	51	from	swell	693:697	arg1	interface					718:726	the interface	714:726	the interface due to their deformable structure	714:760	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	5	52	theme	Pickering	910:918	arg1	emulsions					920:928	Pickering emulsions	910:928	Pickering emulsions	910:928	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	5	53	theme	interfacial	858:868	arg1	structure					870:878	interfacial structure	858:878	interfacial structure	858:878	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	5	54	theme	emulsions	920:928	arg1	structure					870:878	interfacial structure	858:878	interfacial structure	858:878	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	5	54	theme	emulsions	920:928	arg1	properties					896:905	macroscopic properties	884:905	macroscopic properties	884:905	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	1	55	theme	alginate	220:227	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	55	theme	alginate	220:227	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	7	56	theme	small	1240:1244	arg1	phase					1257:1261	the small intestinal phase	1236:1261	the small intestinal phase	1236:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	1	57	theme	β-carotene	394:403	arg1	delivery					382:389	delivery	382:389	delivery of β-carotene	382:403	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	3	58	theme	interparticle	653:665	arg1	gaps					667:670	the substantial interparticle gaps	637:670	the substantial interparticle gaps	637:670	Microstructural observations showed that ZPCPs were effectively adsorbed at the oil/water interface despite the substantial interparticle gaps.					
34617943	5	59	theme	ratio	967:971	arg1	sequence					986:993	the mass ratio and addition sequence	958:993	the mass ratio and addition sequence of different particles	958:1016	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	9	60	theme	particle-particle	1520:1536	arg1	interfaces					1546:1555	particle-particle complex interfaces	1520:1555	particle-particle complex interfaces	1520:1555	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	0	61	theme	Pickering	82:90	arg1	emulsions					92:100	Pickering emulsions	82:100	Pickering emulsions	82:100	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	1	62	theme	zein-propylene	198:211	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	62	theme	zein-propylene	198:211	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	8	63	theme	β-carotene	1463:1472	arg1	bioaccessibility					1474:1489	β-carotene bioaccessibility	1463:1489	β-carotene bioaccessibility	1463:1489	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	64	theme	inner	1325:1329	arg1	layer					1331:1335	the inner layer	1321:1335	the inner layer	1321:1335	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	64	theme	inner	1325:1329	arg1	ZPCPs					1302:1306	ZPCPs	1302:1306	ZPCPs	1302:1306	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	7	65	theme	intestinal	1246:1255	arg1	phase					1257:1261	the small intestinal phase	1236:1261	the small intestinal phase	1236:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	1	66	theme	large	168:172	arg1	ZPCPs					250:254	ZPCPs	250:254	ZPCPs	250:254	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	1	66	theme	large	168:172	arg1	particles					239:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles	168:247	large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs)	168:255	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	6	67	theme	WPM	1048:1050	arg1	combination					1023:1033	The combination	1019:1033	The combination of ZPCPs and WPM	1019:1050	The combination of ZPCPs and WPM delayed the lipolysis during gastrointestinal digestion.					
34617943	0	68	theme	complex	125:131	arg1	interfaces					133:142	particle-particle complex interfaces	107:142	particle-particle complex interfaces	107:142	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	5	69	theme	particles	1008:1016	arg1	sequence					986:993	the mass ratio and addition sequence	958:993	the mass ratio and addition sequence of different particles	958:1016	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	6	70	theme	ZPCPs	1038:1042	arg1	combination					1023:1033	The combination	1019:1033	The combination of ZPCPs and WPM	1019:1050	The combination of ZPCPs and WPM delayed the lipolysis during gastrointestinal digestion.					
34617943	7	71	from	rate	1205:1208	arg1	phase					1257:1261	the small intestinal phase	1236:1261	the small intestinal phase	1236:1261	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	2	72	theme	different	508:516	arg1	particles					518:526	different particles	508:526	different particles	508:526	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	4	73	theme	flattened	803:811	arg1	particles					813:821	flattened particles	803:821	flattened particles	803:821	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	7	74	theme	Pickering	1213:1221	arg1	emulsions					1223:1231	Pickering emulsions	1213:1231	Pickering emulsions	1213:1231	Through controlling the composition of the complex interface, the free fatty acid (FFA) release rate of Pickering emulsions in the small intestinal phase was reduced from 15.64% to 9.03%.					
34617943	5	75	theme	addition	977:984	arg1	sequence					986:993	the mass ratio and addition sequence	958:993	the mass ratio and addition sequence of different particles	958:1016	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	0	76	theme	controlled	23:32	arg1	digestion					51:59	controlled gastrointestinal digestion	23:59	controlled gastrointestinal digestion	23:59	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	8	77	theme	mass	1372:1375	arg1	 1					1406:1407	 1	1406:1407	 1	1406:1407	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	77	theme	mass	1372:1375	arg1	ratio					1377:1381	the mass ratio	1368:1381	the mass ratio of ZPCPs to WPM	1368:1397	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	2	78	theme	photothermal	410:421	arg1	stability					423:431	photothermal stability	410:431	photothermal stability	410:431	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	1	79	theme	whey	290:293	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	4	80	theme	surface	840:846	arg1	area					848:851	a large surface area	832:851	a large surface area	832:851	WPM particles could swell and stretch on the interface due to their deformable structure, thereby forming an interfacial layer of flattened particles to cover a large surface area.					
34617943	0	81	theme	β-carotene	64:73	arg1	digestion					51:59	controlled gastrointestinal digestion	23:59	controlled gastrointestinal digestion	23:59	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	0	81	theme	β-carotene	64:73	arg1	stability					9:17	Enhanced stability	0:17	Enhanced stability	0:17	Enhanced stability and controlled gastrointestinal digestion of β-carotene loaded Pickering emulsions with particle-particle complex interfaces.					
34617943	1	82	theme	microgel	303:310	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	2	83	theme	β-carotene	458:467	arg1	stability					445:453	storage stability	437:453	storage stability	437:453	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	2	83	theme	β-carotene	458:467	arg1	stability					423:431	photothermal stability	410:431	photothermal stability	410:431	The photothermal stability and storage stability of β-carotene were improved with the combined use of different particles.					
34617943	1	84	theme	WPM	313:315	arg1	particles					318:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	small, soft, and hydrophobic whey protein microgel (WPM) particles	261:326	In this study, we used large, rigid, and hydrophilic zein-propylene glycol alginate composite particles (ZPCPs) and small, soft, and hydrophobic whey protein microgel (WPM) particles to synergistically stabilize a Pickering emulsion for delivery of β-carotene.					
34617943	8	85	theme	outer	1352:1356	arg1	layer					1358:1362	the outer layer	1348:1362	the outer layer	1348:1362	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	8	85	theme	outer	1352:1356	arg1	ZPCPs					1302:1306	ZPCPs	1302:1306	ZPCPs	1302:1306	When ZPCPs were used as the inner layer and WPM as the outer layer and the mass ratio of ZPCPs to WPM was 4 : 1, the Pickering emulsion showed the best stability and β-carotene bioaccessibility.					
34617943	5	86	theme	macroscopic	884:894	arg1	properties					896:905	macroscopic properties	884:905	macroscopic properties	884:905	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	9	87	theme	enhanced	1622:1629	arg1	stability					1631:1639	enhanced stability	1622:1639	enhanced stability	1622:1639	The Pickering emulsion with particle-particle complex interfaces could be applied in foods and pharmaceuticals for the purpose of enhanced stability, delayed lipolysis or sustained nutrient release.					
34617943	5	88	dep	structure	870:878	arg1	The					854:856	The	854:856	The	854:856	The interfacial structure and macroscopic properties of Pickering emulsions were modulated by adjusting the mass ratio and addition sequence of different particles.					
34617943	3	89	theme	Microstructural	529:543	arg1	observations					545:556	Microstructural observations	529:556	Microstructural observations	529:556	Microstructural observations showed that ZPCPs were effectively adsorbed at the oil/water interface despite the substantial interparticle gaps.					
33682152	9	0	theme	volatiles	1205:1213	arg1	degradation					1190:1200	degradation	1190:1200	a creation as well as degradation of volatiles	1168:1213	Fermentation contributed to a creation as well as degradation of volatiles.					
33682152	9	0	theme	volatiles	1205:1213	arg1	creation					1170:1177	a creation	1168:1177	a creation as well as degradation of volatiles	1168:1213	Fermentation contributed to a creation as well as degradation of volatiles.					
33682152	13	1	theme	kvass	1717:1721	arg1	production					1703:1712	production	1703:1712	production of kvass with probiotic properties	1703:1747	Moreover, S. boulardii is a better choice for production of kvass with probiotic properties.					
33682152	6	2	from	content	868:874	arg1	samples					879:885	samples	879:885	samples	879:885	Moreover, fermentation efficiently reduced furfural and hydroxymethylfurfural content in samples up to 12.9% and 29.9%, respectively.					
33682152	10	3	theme	higher	1239:1244	arg1	mortality					1254:1262	higher general mortality	1239:1262	higher general mortality	1239:1262	L. plantarum exhibited higher general mortality during storage, whereas, in the case of S. boulardii, the viability was significantly higher regardless of the sample composition.					
33682152	0	4	theme	kvass	63:67	arg1	production					49:58	production	49:58	production of kvass with probiotic potential	49:92	Carob kibbles as an alternative raw material for production of kvass with probiotic potential.					
33682152	12	5	theme	health	1640:1645	arg1	benefits					1647:1654	added health benefits	1634:1654	added health benefits	1634:1654	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
33682152	7	6	theme	chocolate-like	977:990	arg1	odours					1018:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	1	7	theme	great	159:163	arg1	interest					165:172	great interest	159:172	great interest	159:172	BACKGROUND Non-diary beverages with probiotic properties are of great interest nowadays.					
33682152	5	8	theme	antiradical	739:749	arg1	activity					751:758	antiradical activity	739:758	antiradical activity	739:758	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	4	9	theme	boulardii	510:518	arg1	strains					457:463	probiotic strains	447:463	probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii	447:518	Therefore, the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages was tested.					
33682152	5	10	theme	gallic	682:687	arg1	kvasses					662:668	kvasses	662:668	kvasses	662:668	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	5	10	theme	gallic	682:687	arg1	acid					689:692	gallic acid	682:692	gallic acid (up to 117.45 ± 10.56 mg L-1 )	682:723	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	5	11	theme	significant	615:625	arg1	amounts					627:633	significant amounts	615:633	significant amounts of phenolic compounds	615:655	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	5	11	theme	significant	615:625	arg1	compounds					647:655	phenolic compounds	638:655	phenolic compounds	638:655	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	7	12	theme	rye	937:939	arg1	extract					946:952	rye malt extract	937:952	rye malt extract	937:952	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	12	13	theme	malt	1550:1553	arg1	extract					1555:1561	rye malt extract	1546:1561	rye malt extract	1546:1561	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
33682152	0	14	theme	probiotic	74:82	arg1	potential					84:92	probiotic potential	74:92	probiotic potential	74:92	Carob kibbles as an alternative raw material for production of kvass with probiotic potential.					
33682152	14	15	theme	Chemical	1768:1775	arg1	Industry					1777:1784	Chemical Industry	1768:1784	Chemical Industry	1768:1784	© 2021 Society of Chemical Industry.					
33682152	3	16	with	beverage	383:390	arg1	properties					407:416	probiotic properties	397:416	probiotic properties	397:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	7	17	theme	coffee-like	964:974	arg1	odours					1018:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	2	18	theme	kvass	270:274	arg1	manufacture					255:265	the manufacture	251:265	the manufacture of kvass	251:274	In this research, we evaluated the suitability of carob kibbles in the manufacture of kvass.					
33682152	8	19	with	kvass	1058:1062	arg1	kibbles					1075:1081	carob kibbles	1069:1081	carob kibbles	1069:1081	Whereas kvass with carob kibbles was characterized by fruit-like odour and sweeter taste.					
33682152	12	20	theme	rye	1546:1548	arg1	extract					1555:1561	rye malt extract	1546:1561	rye malt extract	1546:1561	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
33682152	4	21	theme	strains	457:463	arg1	viability					434:442	the viability	430:442	the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages	430:568	Therefore, the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages was tested.					
33682152	5	22	theme	phenolic	638:645	arg1	compounds					647:655	phenolic compounds	638:655	phenolic compounds	638:655	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	0	23	with	production	49:58	arg1	potential					84:92	probiotic potential	74:92	probiotic potential	74:92	Carob kibbles as an alternative raw material for production of kvass with probiotic potential.					
33682152	11	24	theme	carob	1451:1455	arg1	kibbles					1457:1463	carob kibbles	1451:1463	carob kibbles	1451:1463	CONCLUSION This is the first study reporting the use of carob kibbles for kvass production.					
33682152	4	25	from	storage	536:542	arg1	beverages					560:568	manufactured beverages	547:568	manufactured beverages	547:568	Therefore, the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages was tested.					
33682152	3	26	theme	Eastern	329:335	arg1	Europe					337:342	Central and Eastern Europe	317:342	Central and Eastern Europe	317:342	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	4	27	theme	plantarum	482:490	arg1	strains					457:463	probiotic strains	447:463	probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii	447:518	Therefore, the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages was tested.					
33682152	5	28	theme	compounds	647:655	arg1	amounts					627:633	significant amounts	615:633	significant amounts of phenolic compounds	615:655	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	5	28	theme	compounds	647:655	arg1	compounds					647:655	phenolic compounds	638:655	phenolic compounds	638:655	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	5	29	dep	acid	689:692	arg1	up					695:696	up	695:696	up to 117.45 ± 10.56 mg L-1	695:721	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	3	30	theme	popular	306:312	arg1	drink					300:304	a low-alcohol drink	286:304	a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties	286:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	3	30	theme	popular	306:312	arg1	Kvass					277:281	Kvass	277:281	Kvass	277:281	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	8	31	theme	Whereas	1050:1056	arg1	kvass					1058:1062	kvass	1058:1062	Whereas kvass with carob kibbles	1050:1081	Whereas kvass with carob kibbles was characterized by fruit-like odour and sweeter taste.					
33682152	5	32	theme	RESULTS	582:588	arg1	kibbles					596:602	RESULTS Carob kibbles	582:602	RESULTS Carob kibbles	582:602	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	2	33	from	suitability	219:229	arg1	manufacture					255:265	the manufacture	251:265	the manufacture of kvass	251:274	In this research, we evaluated the suitability of carob kibbles in the manufacture of kvass.					
33682152	3	34	theme	potential	363:371	arg1	beverage					383:390	a potential non-diary beverage	361:390	a potential non-diary beverage with probiotic properties	361:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	1	35	with	beverages	116:124	arg1	properties					141:150	probiotic properties	131:150	probiotic properties	131:150	BACKGROUND Non-diary beverages with probiotic properties are of great interest nowadays.					
33682152	5	36	theme	Carob	590:594	arg1	kibbles					596:602	RESULTS Carob kibbles	582:602	RESULTS Carob kibbles	582:602	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	8	37	theme	carob	1069:1073	arg1	kibbles					1075:1081	carob kibbles	1069:1081	carob kibbles	1069:1081	Whereas kvass with carob kibbles was characterized by fruit-like odour and sweeter taste.					
33682152	11	38	theme	kvass	1469:1473	arg1	production					1475:1484	kvass production	1469:1484	kvass production	1469:1484	CONCLUSION This is the first study reporting the use of carob kibbles for kvass production.					
33682152	3	39	theme	indicated	348:356	arg1	drink					300:304	a low-alcohol drink	286:304	a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties	286:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	3	39	theme	indicated	348:356	arg1	Kvass					277:281	Kvass	277:281	Kvass	277:281	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	4	40	theme	manufactured	547:558	arg1	beverages					560:568	manufactured beverages	547:568	manufactured beverages	547:568	Therefore, the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages was tested.					
33682152	12	41	theme	kvass	1605:1609	arg1	production					1591:1600	the production	1587:1600	the production of kvass	1587:1609	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
33682152	7	42	theme	malt	941:944	arg1	extract					946:952	rye malt extract	937:952	rye malt extract	937:952	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	13	43	theme	better	1685:1690	arg1	boulardii					1670:1678	S. boulardii	1667:1678	S. boulardii	1667:1678	Moreover, S. boulardii is a better choice for production of kvass with probiotic properties.					
33682152	13	43	theme	better	1685:1690	arg1	choice					1692:1697	a better choice	1683:1697	a better choice for production of kvass with probiotic properties	1683:1747	Moreover, S. boulardii is a better choice for production of kvass with probiotic properties.					
33682152	11	44	dep	CONCLUSION	1395:1404	arg1	This					1406:1409	This	1406:1409	This	1406:1409	CONCLUSION This is the first study reporting the use of carob kibbles for kvass production.					
33682152	1	45	theme	BACKGROUND	95:104	arg1	beverages					116:124	BACKGROUND Non-diary beverages	95:124	BACKGROUND Non-diary beverages with probiotic properties	95:150	BACKGROUND Non-diary beverages with probiotic properties are of great interest nowadays.					
33682152	11	46	theme	kibbles	1457:1463	arg1	use					1444:1446	the use	1440:1446	the use of carob kibbles for kvass production	1440:1484	CONCLUSION This is the first study reporting the use of carob kibbles for kvass production.					
33682152	0	47	theme	raw	32:34	arg1	material					36:43	an alternative raw material	17:43	an alternative raw material for production of kvass with probiotic potential	17:92	Carob kibbles as an alternative raw material for production of kvass with probiotic potential.					
33682152	7	48	with	Kvasses	924:930	arg1	extract					946:952	rye malt extract	937:952	rye malt extract	937:952	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	5	49	dep	78	766:767	arg1	to					763:764	to	763:764	to	763:764	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	10	50	theme	boulardii	1307:1315	arg1	case					1296:1299	the case	1292:1299	the case of S. boulardii	1292:1315	L. plantarum exhibited higher general mortality during storage, whereas, in the case of S. boulardii, the viability was significantly higher regardless of the sample composition.					
33682152	0	51	theme	alternative	20:30	arg1	material					36:43	an alternative raw material	17:43	an alternative raw material for production of kvass with probiotic potential	17:92	Carob kibbles as an alternative raw material for production of kvass with probiotic potential.					
33682152	12	52	theme	added	1634:1638	arg1	benefits					1647:1654	added health benefits	1634:1654	added health benefits	1634:1654	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
33682152	3	53	theme	probiotic	397:405	arg1	properties					407:416	probiotic properties	397:416	probiotic properties	397:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	3	54	from	Europe	337:342	arg1	popular					306:312	popular	306:312	popular	306:312	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	7	55	contain	possessed	954:962	arg2	taste					1043:1047	a more bitter taste	1029:1047	a more bitter taste	1029:1047	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	7	55	contain	possessed	954:962	arg2	odours					1018:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	7	55	contain	possessed	954:962	arg1	Kvasses					924:930	Kvasses	924:930	Kvasses with rye malt extract	924:952	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	7	56	theme	more	1031:1034	arg1	bitter					1036:1041	a more bitter	1029:1041	a more bitter taste	1029:1047	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	11	57	theme	first	1418:1422	arg1	study					1424:1428	the first study	1414:1428	the first study reporting the use of carob kibbles for kvass production	1414:1484	CONCLUSION This is the first study reporting the use of carob kibbles for kvass production.					
33682152	11	57	theme	first	1418:1422	arg1	CONCLUSION					1395:1404	CONCLUSION	1395:1404	CONCLUSION This	1395:1409	CONCLUSION This is the first study reporting the use of carob kibbles for kvass production.					
33682152	3	58	theme	non-diary	373:381	arg1	beverage					383:390	a potential non-diary beverage	361:390	a potential non-diary beverage with probiotic properties	361:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	8	59	theme	sweeter	1125:1131	arg1	taste					1133:1137	sweeter taste	1125:1137	sweeter taste	1125:1137	Whereas kvass with carob kibbles was characterized by fruit-like odour and sweeter taste.					
33682152	12	60	theme	carob	1520:1524	arg1	kibbles					1526:1532	carob kibbles	1520:1532	carob kibbles	1520:1532	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
33682152	7	61	theme	bitter	1036:1041	arg1	taste					1043:1047	a more bitter taste	1029:1047	a more bitter taste	1029:1047	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	1	62	theme	Non-diary	106:114	arg1	beverages					116:124	BACKGROUND Non-diary beverages	95:124	BACKGROUND Non-diary beverages with probiotic properties	95:150	BACKGROUND Non-diary beverages with probiotic properties are of great interest nowadays.					
33682152	5	63	dep	%	768:768	arg1	fermentation					776:787	fermentation	776:787	fermentation	776:787	RESULTS Carob kibbles introduced significant amounts of phenolic compounds into kvasses, especially gallic acid (up to 117.45 ± 10.56 mg L-1 ), and improved antiradical activity up to 78% after fermentation.					
33682152	3	64	theme	low-alcohol	288:298	arg1	drink					300:304	a low-alcohol drink	286:304	a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties	286:416	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	3	64	theme	low-alcohol	288:298	arg1	Kvass					277:281	Kvass	277:281	Kvass	277:281	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	4	65	theme	probiotic	447:455	arg1	strains					457:463	probiotic strains	447:463	probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii	447:518	Therefore, the viability of probiotic strains of Lactobacillus plantarum and Saccharomyces boulardii during 4 weeks' storage in manufactured beverages was tested.					
33682152	3	66	from	popular	306:312	arg1	Europe					337:342	Central and Eastern Europe	317:342	Central and Eastern Europe	317:342	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	6	67	theme	furfural	833:840	arg1	content					868:874	furfural and hydroxymethylfurfural content	833:874	furfural and hydroxymethylfurfural content in samples	833:885	Moreover, fermentation efficiently reduced furfural and hydroxymethylfurfural content in samples up to 12.9% and 29.9%, respectively.					
33682152	13	68	theme	probiotic	1728:1736	arg1	properties					1738:1747	probiotic properties	1728:1747	probiotic properties	1728:1747	Moreover, S. boulardii is a better choice for production of kvass with probiotic properties.					
33682152	13	69	with	production	1703:1712	arg1	properties					1738:1747	probiotic properties	1728:1747	probiotic properties	1728:1747	Moreover, S. boulardii is a better choice for production of kvass with probiotic properties.					
33682152	7	70	theme	caramel-like	1005:1016	arg1	odours					1018:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	1	71	theme	probiotic	131:139	arg1	properties					141:150	probiotic properties	131:150	probiotic properties	131:150	BACKGROUND Non-diary beverages with probiotic properties are of great interest nowadays.					
33682152	6	72	theme	hydroxymethylfurfural	846:866	arg1	content					868:874	furfural and hydroxymethylfurfural content	833:874	furfural and hydroxymethylfurfural content in samples	833:885	Moreover, fermentation efficiently reduced furfural and hydroxymethylfurfural content in samples up to 12.9% and 29.9%, respectively.					
33682152	2	73	theme	kibbles	240:246	arg1	suitability					219:229	the suitability	215:229	the suitability of carob kibbles in the manufacture of kvass	215:274	In this research, we evaluated the suitability of carob kibbles in the manufacture of kvass.					
33682152	10	74	theme	sample	1375:1380	arg1	composition					1382:1392	the sample composition	1371:1392	the sample composition	1371:1392	L. plantarum exhibited higher general mortality during storage, whereas, in the case of S. boulardii, the viability was significantly higher regardless of the sample composition.					
33682152	10	75	theme	general	1246:1252	arg1	mortality					1254:1262	higher general mortality	1239:1262	higher general mortality	1239:1262	L. plantarum exhibited higher general mortality during storage, whereas, in the case of S. boulardii, the viability was significantly higher regardless of the sample composition.					
33682152	3	76	theme	Central	317:323	arg1	Europe					337:342	Central and Eastern Europe	317:342	Central and Eastern Europe	317:342	Kvass is a low-alcohol drink popular in Central and Eastern Europe and indicated as a potential non-diary beverage with probiotic properties.					
33682152	2	77	theme	carob	234:238	arg1	kibbles					240:246	carob kibbles	234:246	carob kibbles	234:246	In this research, we evaluated the suitability of carob kibbles in the manufacture of kvass.					
33682152	7	78	theme	roasted	993:999	arg1	odours					1018:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	coffee-like, chocolate-like, roasted and caramel-like odours	964:1023	Kvasses with rye malt extract possessed coffee-like, chocolate-like, roasted and caramel-like odours and a more bitter taste.					
33682152	8	79	theme	fruit-like	1104:1113	arg1	odour					1115:1119	fruit-like odour	1104:1119	fruit-like odour	1104:1119	Whereas kvass with carob kibbles was characterized by fruit-like odour and sweeter taste.					
33682152	12	80	theme	obtained	1491:1498	arg1	results					1500:1506	The obtained results	1487:1506	The obtained results	1487:1506	The obtained results showed that carob kibbles can replace rye malt extract, at least partially, in the production of kvass, giving to the product added health benefits.					
32410075	4	0	theme	network	632:638	arg1	model					640:644	a novel network model	624:644	a novel network model	624:644	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	6	1	theme	hysteresis	1230:1239	arg1	response					1241:1248	the hysteresis response	1226:1248	the hysteresis response	1226:1248	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	6	1	theme	hysteresis	1230:1239	arg1	simulated					1259:1267	simulated	1259:1267	simulated	1259:1267	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	7	2	theme	relative	1395:1402	arg1	contribution					1415:1426	their relative mechanical contribution	1389:1426	their relative mechanical contribution to the larger scale tissue mechanical response	1389:1473	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	7	3	theme	larger	1435:1440	arg1	response					1466:1473	the larger scale tissue mechanical response	1431:1473	the larger scale tissue mechanical response	1431:1473	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	5	4	theme	mechanical	1053:1062	arg1	response					1064:1071	the network model's mechanical response	1033:1071	the network model's mechanical response	1033:1071	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	1	5	theme	lung	146:149	arg1	tissue					163:168	the lung parenchymal tissue	142:168	the lung parenchymal tissue	142:168	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	1	6	theme	lung	238:241	arg1	properties					220:229	the mechanical properties	205:229	the mechanical properties of the lung	205:241	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	4	7	theme	derived	819:825	arg1	response					842:849	the experimentally derived length-tension response	800:849	the experimentally derived length-tension response of the tissue to uniaxial loading	800:883	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	8	theme	ECM	715:717	arg1	proteoglycans					762:774	proteoglycans	762:774	proteoglycans (PGs)	762:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	8	theme	ECM	715:717	arg1	elastin					750:756	elastin	750:756	elastin	750:756	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	8	theme	ECM	715:717	arg1	constituents					719:730	lung tissue ECM constituents	703:730	lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs)	703:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	8	theme	ECM	715:717	arg1	collagen					740:747	collagen	740:747	collagen	740:747	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	3	9	from	impact	514:519	arg1	properties					560:569	the mechanical properties	545:569	the mechanical properties of the tissue	545:583	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	3	10	theme	alterations	530:540	arg1	impact					514:519	the impact	510:519	the impact of these alterations on the mechanical properties of the tissue	510:583	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	5	11	theme	elastin	1024:1030	arg1	response					1012:1019	the linear elastic response	993:1019	the linear elastic response of elastin	993:1030	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	4	12	theme	tissue	708:713	arg1	proteoglycans					762:774	proteoglycans	762:774	proteoglycans (PGs)	762:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	12	theme	tissue	708:713	arg1	elastin					750:756	elastin	750:756	elastin	750:756	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	12	theme	tissue	708:713	arg1	constituents					719:730	lung tissue ECM constituents	703:730	lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs)	703:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	12	theme	tissue	708:713	arg1	collagen					740:747	collagen	740:747	collagen	740:747	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	2	13	with	scaffold	285:292	arg1	composition					334:344	composition	334:344	composition	334:344	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	2	13	with	scaffold	285:292	arg1	structure					320:328	a complex ECM matrix structure	299:328	a complex ECM matrix structure	299:328	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	7	14	theme	mechanical	1404:1413	arg1	contribution					1415:1426	their relative mechanical contribution	1389:1426	their relative mechanical contribution to the larger scale tissue mechanical response	1389:1473	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	5	15	from	literature	1124:1133	arg1	curves					1108:1113	experimental stress-strain curves	1081:1113	experimental stress-strain curves from the literature	1081:1133	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	5	16	theme	collagen	913:920	arg1	elasticity					922:931	collagen elasticity	913:931	collagen elasticity	913:931	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	1	17	theme	parenchymal	151:161	arg1	tissue					163:168	the lung parenchymal tissue	142:168	the lung parenchymal tissue	142:168	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	2	18	theme	lung	248:251	arg1	scaffold					285:292	a biologically active scaffold	263:292	a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells	263:400	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	2	18	theme	lung	248:251	arg1	tissue					253:258	The lung tissue	244:258	The lung tissue	244:258	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	1	19	theme	tissue	163:168	arg1	proportion					128:137	a large proportion	120:137	a large proportion of the lung parenchymal tissue	120:168	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	0	20	theme	viscoelastic	2:13	arg1	model					39:43	A viscoelastic two-dimensional network model	0:43	A viscoelastic two-dimensional network model of the lung extracellular matrix	0:76	A viscoelastic two-dimensional network model of the lung extracellular matrix.					
32410075	5	21	theme	network	1037:1043	arg1	model					1045:1049	the network model's	1033:1051	the network model's mechanical response	1033:1071	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	1	22	theme	extracellular	83:95	arg1	matrix					97:102	The extracellular matrix	79:102	The extracellular matrix (ECM)	79:108	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	1	22	theme	extracellular	83:95	arg1	ECM					105:107	ECM	105:107	ECM	105:107	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	5	23	with	concert	980:986	arg1	response					1012:1019	the linear elastic response	993:1019	the linear elastic response of elastin	993:1030	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	2	24	theme	physical	360:367	arg1	support					369:375	physical support	360:375	physical support to the surrounding cells	360:400	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	4	25	theme	combinatorial	679:691	arg1	effect					693:698	the combinatorial effect	675:698	the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs)	675:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	5	26	theme	elasticity	922:931	arg1	function					951:958	an exponential function	936:958	an exponential function with strain	936:970	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	5	26	theme	elasticity	922:931	arg1	effect					903:908	the effect	899:908	the effect of collagen elasticity	899:931	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	0	27	theme	network	31:37	arg1	model					39:43	A viscoelastic two-dimensional network model	0:43	A viscoelastic two-dimensional network model of the lung extracellular matrix	0:76	A viscoelastic two-dimensional network model of the lung extracellular matrix.					
32410075	2	28	theme	active	278:283	arg1	scaffold					285:292	a biologically active scaffold	263:292	a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells	263:400	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	2	28	theme	active	278:283	arg1	tissue					253:258	The lung tissue	244:258	The lung tissue	244:258	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	7	29	theme	different	1327:1335	arg1	constituents					1341:1352	the different ECM constituents	1323:1352	the different ECM constituents	1323:1352	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	0	30	theme	two-dimensional	15:29	arg1	model					39:43	A viscoelastic two-dimensional network model	0:43	A viscoelastic two-dimensional network model of the lung extracellular matrix	0:76	A viscoelastic two-dimensional network model of the lung extracellular matrix.					
32410075	4	31	theme	tissue	858:863	arg1	response					842:849	the experimentally derived length-tension response	800:849	the experimentally derived length-tension response of the tissue to uniaxial loading	800:883	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	5	32	theme	linear	997:1002	arg1	response					1012:1019	the linear elastic response	993:1019	the linear elastic response of elastin	993:1030	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	1	33	theme	important	180:188	arg1	contributor					190:200	an important contributor	177:200	an important contributor to the mechanical properties of the lung	177:241	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	5	34	theme	elastic	1004:1010	arg1	response					1012:1019	the linear elastic response	993:1019	the linear elastic response of elastin	993:1030	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	7	35	theme	constituents	1341:1352	arg1	fractions					1310:1318	volume fractions	1303:1318	volume fractions of the different ECM constituents	1303:1352	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	2	36	theme	surrounding	384:394	arg1	cells					396:400	the surrounding cells	380:400	the surrounding cells	380:400	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	3	37	theme	respiratory	414:424	arg1	pathologies					426:436	Nearly all respiratory pathologies	403:436	Nearly all respiratory pathologies	403:436	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	4	38	used	used	786:789	arg2	model					640:644	a novel network model	624:644	a novel network model	624:644	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	39	theme	uniaxial	868:875	arg1	loading					877:883	uniaxial loading	868:883	uniaxial loading	868:883	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	6	40	theme	viscoelastic	1181:1192	arg1	elements					1194:1201	spring-dashpot viscoelastic elements	1166:1201	spring-dashpot viscoelastic elements	1166:1201	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	0	41	theme	lung	52:55	arg1	matrix					71:76	the lung extracellular matrix	48:76	the lung extracellular matrix	48:76	A viscoelastic two-dimensional network model of the lung extracellular matrix.					
32410075	5	42	theme	experimental	1081:1092	arg1	curves					1108:1113	experimental stress-strain curves	1081:1113	experimental stress-strain curves from the literature	1081:1133	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	5	43	with	function	951:958	arg1	strain					965:970	strain	965:970	strain	965:970	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	6	44	theme	spring-dashpot	1166:1179	arg1	elements					1194:1201	spring-dashpot viscoelastic elements	1166:1201	spring-dashpot viscoelastic elements	1166:1201	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	2	45	theme	matrix	313:318	arg1	structure					320:328	a complex ECM matrix structure	299:328	a complex ECM matrix structure	299:328	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	5	46	theme	stress-strain	1094:1106	arg1	curves					1108:1113	experimental stress-strain curves	1081:1113	experimental stress-strain curves from the literature	1081:1133	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	4	47	theme	length-tension	827:840	arg1	response					842:849	the experimentally derived length-tension response	800:849	the experimentally derived length-tension response of the tissue to uniaxial loading	800:883	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	7	48	theme	mechanical	1455:1464	arg1	response					1466:1473	the larger scale tissue mechanical response	1431:1473	the larger scale tissue mechanical response	1431:1473	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	3	49	theme	mechanical	549:558	arg1	properties					560:569	the mechanical properties	545:569	the mechanical properties of the tissue	545:583	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	3	50	from	changes	448:454	arg1	composition					477:487	composition	477:487	composition	477:487	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	3	50	from	changes	448:454	arg1	structure					463:471	structure	463:471	structure	463:471	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	2	51	theme	ECM	309:311	arg1	structure					320:328	a complex ECM matrix structure	299:328	a complex ECM matrix structure	299:328	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	7	52	theme	volume	1303:1308	arg1	fractions					1310:1318	volume fractions	1303:1318	volume fractions of the different ECM constituents	1303:1352	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	6	53	from	simulated	1259:1267	arg1	addition					1139:1146	addition	1139:1146	addition	1139:1146	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	4	54	theme	novel	626:630	arg1	model					640:644	a novel network model	624:644	a novel network model	624:644	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	2	55	theme	complex	301:307	arg1	structure					320:328	a complex ECM matrix structure	299:328	a complex ECM matrix structure	299:328	The lung tissue is a biologically active scaffold with a complex ECM matrix structure and composition that provides physical support to the surrounding cells.					
32410075	4	56	theme	lung	703:706	arg1	proteoglycans					762:774	proteoglycans	762:774	proteoglycans (PGs)	762:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	56	theme	lung	703:706	arg1	elastin					750:756	elastin	750:756	elastin	750:756	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	56	theme	lung	703:706	arg1	constituents					719:730	lung tissue ECM constituents	703:730	lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs)	703:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	56	theme	lung	703:706	arg1	collagen					740:747	collagen	740:747	collagen	740:747	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	4	57	theme	constituents	719:730	arg1	effect					693:698	the combinatorial effect	675:698	the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs)	675:780	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	7	58	theme	scale	1442:1446	arg1	response					1466:1473	the larger scale tissue mechanical response	1431:1473	the larger scale tissue mechanical response	1431:1473	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	1	59	theme	large	122:126	arg1	proportion					128:137	a large proportion	120:137	a large proportion of the lung parenchymal tissue	120:168	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	1	60	theme	mechanical	209:218	arg1	properties					220:229	the mechanical properties	205:229	the mechanical properties of the lung	205:241	The extracellular matrix (ECM) comprises a large proportion of the lung parenchymal tissue and is an important contributor to the mechanical properties of the lung.					
32410075	0	61	theme	matrix	71:76	arg1	model					39:43	A viscoelastic two-dimensional network model	0:43	A viscoelastic two-dimensional network model of the lung extracellular matrix	0:76	A viscoelastic two-dimensional network model of the lung extracellular matrix.					
32410075	7	62	theme	tissue	1448:1453	arg1	response					1466:1473	the larger scale tissue mechanical response	1431:1473	the larger scale tissue mechanical response	1431:1473	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32410075	5	63	theme	exponential	939:949	arg1	function					951:958	an exponential function	936:958	an exponential function with strain	936:970	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	5	63	theme	exponential	939:949	arg1	effect					903:908	the effect	899:908	the effect of collagen elasticity	899:931	By modelling the effect of collagen elasticity as an exponential function with strain, and in concert with the linear elastic response of elastin, the network model's mechanical response matched experimental stress-strain curves from the literature.					
32410075	3	64	theme	ECM	496:498	arg1	composition					477:487	composition	477:487	composition	477:487	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	3	64	theme	ECM	496:498	arg1	structure					463:471	structure	463:471	structure	463:471	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	0	65	theme	extracellular	57:69	arg1	matrix					71:76	the lung extracellular matrix	48:76	the lung extracellular matrix	48:76	A viscoelastic two-dimensional network model of the lung extracellular matrix.					
32410075	3	66	theme	tissue	578:583	arg1	properties					560:569	the mechanical properties	545:569	the mechanical properties of the tissue	545:583	Nearly all respiratory pathologies result in changes in the structure and composition of the ECM; however, the impact of these alterations on the mechanical properties of the tissue is not well understood.					
32410075	4	67	link	derived	819:825	arg1	response					842:849	the experimentally derived length-tension response	800:849	the experimentally derived length-tension response of the tissue to uniaxial loading	800:883	In this study, a novel network model was developed to incorporate the combinatorial effect of lung tissue ECM constituents such as collagen, elastin and proteoglycans (PGs) and used to mimic the experimentally derived length-tension response of the tissue to uniaxial loading.					
32410075	6	68	from	addition	1139:1146	arg1	response					1241:1248	the hysteresis response	1226:1248	the hysteresis response	1226:1248	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	6	68	from	addition	1139:1146	arg1	simulated					1259:1267	simulated	1259:1267	simulated	1259:1267	In addition, by incorporating spring-dashpot viscoelastic elements, to represent the PGs, the hysteresis response was also simulated.					
32410075	7	69	theme	ECM	1337:1339	arg1	constituents					1341:1352	the different ECM constituents	1323:1352	the different ECM constituents	1323:1352	Finally, by selectively reducing volume fractions of the different ECM constituents, we were able to gain insight into their relative mechanical contribution to the larger scale tissue mechanical response.					
32965647	6	0	theme	composition	842:852	arg1	changes					854:860	Tissue glycosaminoglycan composition changes	817:860	Tissue glycosaminoglycan composition changes	817:860	Tissue glycosaminoglycan composition changes are also found.					
32965647	4	1	theme	systemic	614:621	arg1	changes					623:629	systemic changes	614:629	systemic changes in organ function due to loss of XylT2 activity	614:677	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	2	2	theme	significant	314:324	arg1	decrease					326:333	a widespread and significant decrease	297:333	a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-)	297:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	6	3	theme	glycosaminoglycan	824:840	arg1	changes					854:860	Tissue glycosaminoglycan composition changes	817:860	Tissue glycosaminoglycan composition changes	817:860	Tissue glycosaminoglycan composition changes are also found.					
32965647	8	4	theme	PG	1134:1135	arg1	biosynthesis					1137:1148	PG biosynthesis	1134:1148	PG biosynthesis	1134:1148	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	1	5	theme	multiple	226:233	arg1	organs					235:240	multiple organs	226:240	multiple organs	226:240	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	6	6	theme	Tissue	817:822	arg1	changes					854:860	Tissue glycosaminoglycan composition changes	817:860	Tissue glycosaminoglycan composition changes	817:860	Tissue glycosaminoglycan composition changes are also found.					
32965647	4	7	theme	activity	596:603	arg1	loss					577:580	loss	577:580	loss of serum XylT activity	577:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	4	8	theme	activity	670:677	arg1	loss					656:659	loss	656:659	loss of XylT2 activity	656:677	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	1	9	theme	organs	235:240	arg1	biochemistry					210:221	the proteoglycan (PG) biochemistry	188:221	the proteoglycan (PG) biochemistry of multiple organs	188:240	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	8	10	theme	residual	982:989	arg1	due					1024:1026	due	1024:1026	due	1024:1026	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	8	10	theme	residual	982:989	arg1	activity					996:1003	the residual XylT activity	978:1003	the residual XylT activity in the Xylt2-/-	978:1019	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	4	11	theme	XylT2	664:668	arg1	activity					670:677	XylT2 activity	664:677	XylT2 activity	664:677	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	4	12	theme	organ	634:638	arg1	function					640:647	organ function	634:647	organ function	634:647	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	2	13	theme	widespread	299:308	arg1	decrease					326:333	a widespread and significant decrease	297:333	a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-)	297:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	5	14	theme	red	742:744	arg1	area					751:754	the red pulp area	738:754	the red pulp area	738:754	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	4	15	theme	XylT	591:594	arg1	activity					596:603	serum XylT activity	585:603	serum XylT activity	585:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	7	16	from	role	906:909	arg1	organs					941:946	multiple organs	932:946	multiple organs	932:946	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	5	17	theme	enhanced	760:767	arg1	response					779:786	enhanced pulmonary response	760:786	enhanced pulmonary response to bacterial liposaccharide	760:814	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	4	18	theme	weight	565:570	arg1	findings					526:533	our previous findings	513:533	our previous findings of increased liver and kidney weight with loss of serum XylT activity	513:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	8	19	theme	XylT	991:994	arg1	due					1024:1026	due	1024:1026	due	1024:1026	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	8	19	theme	XylT	991:994	arg1	activity					996:1003	the residual XylT activity	978:1003	the residual XylT activity in the Xylt2-/-	978:1019	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	2	20	theme	XylT	344:347	arg1	activity					349:356	total XylT activity	338:356	total XylT activity in Xylt2 knock out mice (Xylt2-/-)	338:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	0	21	theme	Xylosyltransferase	0:17	arg1	deficiency					21:30	Xylosyltransferase 2 deficiency	0:30	Xylosyltransferase 2 deficiency	0:30	Xylosyltransferase 2 deficiency and organ homeostasis.					
32965647	4	22	dep	findings	526:533	arg1	addition					501:508	addition	501:508	addition	501:508	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	4	23	theme	due	649:651	arg1	changes					623:629	systemic changes	614:629	systemic changes in organ function due to loss of XylT2 activity	614:677	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	8	24	theme	organ	1154:1158	arg1	homeostasis					1160:1170	organ homeostasis	1154:1170	organ homeostasis	1154:1170	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	2	25	theme	total	338:342	arg1	activity					349:356	total XylT activity	338:356	total XylT activity in Xylt2 knock out mice (Xylt2-/-)	338:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	2	26	from	decrease	326:333	arg1	activity					349:356	total XylT activity	338:356	total XylT activity in Xylt2 knock out mice (Xylt2-/-)	338:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	4	27	theme	kidney	558:563	arg1	weight					565:570	increased liver and kidney weight	538:570	increased liver and kidney weight with loss of serum XylT activity	538:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	4	28	from	changes	623:629	arg1	function					640:647	organ function	634:647	organ function	634:647	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	7	29	theme	multiple	932:939	arg1	organs					941:946	multiple organs	932:946	multiple organs	932:946	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	1	30	theme	Xylosyltransferase	101:118	arg1	function					89:96	the function	85:96	the function of Xylosyltransferase 2 (XylT2) in different tissues	85:149	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	2	31	theme	out	373:375	arg1	mice					377:380	Xylt2 knock out mice	361:380	Xylt2 knock out mice (Xylt2-/-)	361:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	5	32	theme	Xylt2-/-	684:691	arg1	mice					693:696	The Xylt2-/- mice	680:696	The Xylt2-/- mice	680:696	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	5	33	theme	pulp	746:749	arg1	area					751:754	the red pulp area	738:754	the red pulp area	738:754	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	1	34	dep	role	170:173	arg1	has					181:183	has	181:183	has in the proteoglycan (PG) biochemistry of multiple organs	181:240	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	4	35	theme	serum	585:589	arg1	activity					596:603	serum XylT activity	585:603	serum XylT activity	585:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	2	36	theme	knock	367:371	arg1	mice					377:380	Xylt2 knock out mice	361:380	Xylt2 knock out mice (Xylt2-/-)	361:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	0	37	theme	organ	36:40	arg1	homeostasis					42:52	organ homeostasis	36:52	organ homeostasis	36:52	Xylosyltransferase 2 deficiency and organ homeostasis.					
32965647	5	38	theme	pulmonary	769:777	arg1	response					779:786	enhanced pulmonary response	760:786	enhanced pulmonary response to bacterial liposaccharide	760:814	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	7	39	from	content	961:967	arg1	organs					941:946	multiple organs	932:946	multiple organs	932:946	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	5	40	theme	area	751:754	arg1	enlargement					723:733	enlargement	723:733	enlargement of the red pulp area	723:754	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	5	40	theme	area	751:754	arg1	response					779:786	enhanced pulmonary response	760:786	enhanced pulmonary response to bacterial liposaccharide	760:814	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	1	41	theme	proteoglycan	192:203	arg1	biochemistry					210:221	the proteoglycan (PG) biochemistry	188:221	the proteoglycan (PG) biochemistry of multiple organs	188:240	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	2	42	dep	mice	377:380	arg1	Xylt2-/-					383:390	Xylt2-/-	383:390	Xylt2-/-	383:390	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	3	43	theme	weight	435:440	arg1	differences					442:452	increased organ weight differences	419:452	increased organ weight differences in lung, heart, and spleen	419:479	This decrease results in increased organ weight differences in lung, heart, and spleen.					
32965647	8	44	theme	important	1115:1123	arg1	roles					1125:1129	important roles	1115:1129	important roles	1115:1129	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	8	45	contain	have	1110:1113	arg1	XylT1					1094:1098	XylT1	1094:1098	XylT1	1094:1098	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	8	45	contain	have	1110:1113	arg2	roles					1125:1129	important roles	1115:1129	important roles	1115:1129	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	8	45	contain	have	1110:1113	arg1	XylT2					1104:1108	XylT2	1104:1108	XylT2	1104:1108	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	3	46	from	differences	442:452	arg1	spleen					474:479	spleen	474:479	spleen	474:479	This decrease results in increased organ weight differences in lung, heart, and spleen.					
32965647	3	46	from	differences	442:452	arg1	lung					457:460	lung	457:460	lung	457:460	This decrease results in increased organ weight differences in lung, heart, and spleen.					
32965647	3	46	from	differences	442:452	arg1	heart					463:467	heart	463:467	heart	463:467	This decrease results in increased organ weight differences in lung, heart, and spleen.					
32965647	4	47	with	weight	565:570	arg1	loss					577:580	loss	577:580	loss of serum XylT activity	577:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	2	48	from	activity	349:356	arg1	mice					377:380	Xylt2 knock out mice	361:380	Xylt2 knock out mice (Xylt2-/-)	361:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	3	49	theme	increased	419:427	arg1	differences					442:452	increased organ weight differences	419:452	increased organ weight differences in lung, heart, and spleen	419:479	This decrease results in increased organ weight differences in lung, heart, and spleen.					
32965647	7	50	theme	PG	958:959	arg1	content					961:967	their PG content	952:967	their PG content	952:967	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	7	51	theme	XylT2	914:918	arg1	activity					920:927	XylT2 activity	914:927	XylT2 activity	914:927	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	3	52	theme	organ	429:433	arg1	differences					442:452	increased organ weight differences	419:452	increased organ weight differences in lung, heart, and spleen	419:479	This decrease results in increased organ weight differences in lung, heart, and spleen.					
32965647	1	53	theme	PG	206:207	arg1	biochemistry					210:221	the proteoglycan (PG) biochemistry	188:221	the proteoglycan (PG) biochemistry of multiple organs	188:240	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	2	54	theme	Xylt2	361:365	arg1	mice					377:380	Xylt2 knock out mice	361:380	Xylt2 knock out mice (Xylt2-/-)	361:391	The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2-/-).					
32965647	7	55	theme	activity	920:927	arg1	content					961:967	their PG content	952:967	their PG content	952:967	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	7	55	theme	activity	920:927	arg1	role					906:909	a role	904:909	a role of XylT2 activity in multiple organs	904:946	These results demonstrate a role of XylT2 activity in multiple organs and their PG content.					
32965647	5	56	theme	bacterial	791:799	arg1	liposaccharide					801:814	bacterial liposaccharide	791:814	bacterial liposaccharide	791:814	The Xylt2-/- mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide.					
32965647	1	57	from	function	89:96	arg1	tissues					143:149	different tissues	133:149	different tissues	133:149	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	4	58	theme	liver	548:552	arg1	weight					565:570	increased liver and kidney weight	538:570	increased liver and kidney weight with loss of serum XylT activity	538:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	4	59	theme	previous	517:524	arg1	findings					526:533	our previous findings	513:533	our previous findings of increased liver and kidney weight with loss of serum XylT activity	513:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32965647	8	60	from	activity	996:1003	arg1	Xylt2-/-					1012:1019	the Xylt2-/-	1008:1019	the Xylt2-/-	1008:1019	Because the residual XylT activity in the Xylt2-/- is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.					
32965647	1	61	theme	different	133:141	arg1	tissues					143:149	different tissues	133:149	different tissues	133:149	In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs.					
32965647	4	62	theme	increased	538:546	arg1	weight					565:570	increased liver and kidney weight	538:570	increased liver and kidney weight with loss of serum XylT activity	538:603	These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity.					
32944295	0	0	theme	Spike	70:74	arg1	protein					76:82	SARS-CoV-2 Spike protein	59:82	SARS-CoV-2 Spike protein	59:82	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	3	1	theme	flexible	295:302	arg1	loops					304:308	flexible loops	295:308	flexible loops	295:308	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	0	2	theme	SARS-CoV-2	59:68	arg1	protein					76:82	SARS-CoV-2 Spike protein	59:82	SARS-CoV-2 Spike protein	59:82	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	7	3	theme	favourable	1094:1103	arg1	epitopes					1105:1112	favourable epitopes	1094:1112	favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics	1094:1187	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	5	4	theme	antibody-antigen	665:680	arg1	recognition					682:692	antibody-antigen recognition	665:692	antibody-antigen recognition	665:692	The glycan chains alter the accessible surface areas on the S-protein, impeding antibody-antigen recognition.					
32944295	4	5	theme	glycosylation	507:519	arg1	patterns					521:528	six homogeneous glycosylation patterns	491:528	six homogeneous glycosylation patterns	491:528	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	0	6	theme	protein	76:82	arg1	prediction					45:54	the B-cell epitope prediction	26:54	the B-cell epitope prediction of SARS-CoV-2 Spike protein	26:82	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	4	7	theme	experimental	472:483	arg1	data					485:488	published experimental data	462:488	published experimental data	462:488	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	4	8	used	used	562:565	arg2	patterns					521:528	six homogeneous glycosylation patterns	491:528	six homogeneous glycosylation patterns	491:528	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	4	8	used	used	562:565	arg2	ones					552:555	two heterogeneous ones	534:555	two heterogeneous ones	534:555	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	7	9	theme	Spike	944:948	arg1	structure					950:958	the Spike structure	940:958	the Spike structure	940:958	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	3	10	theme	N-glycosylation	318:332	arg1	sites					334:338	all N-glycosylation sites	314:338	all N-glycosylation sites	314:338	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	4	11	theme	published	462:470	arg1	data					485:488	published experimental data	462:488	published experimental data	462:488	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	5	12	theme	surface	624:630	arg1	areas					632:636	the accessible surface areas	609:636	the accessible surface areas on the S-protein	609:653	The glycan chains alter the accessible surface areas on the S-protein, impeding antibody-antigen recognition.					
32944295	5	13	theme	accessible	613:622	arg1	areas					632:636	the accessible surface areas	609:636	the accessible surface areas on the S-protein	609:653	The glycan chains alter the accessible surface areas on the S-protein, impeding antibody-antigen recognition.					
32944295	6	14	from	epitopes	718:725	arg1	subunit					737:743	the S1 subunit	730:743	the S1 subunit	730:743	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	0	15	theme	B-cell	30:35	arg1	prediction					45:54	the B-cell epitope prediction	26:54	the B-cell epitope prediction of SARS-CoV-2 Spike protein	26:82	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	7	16	theme	glycan	1057:1062	arg1	cloud					1064:1068	the glycan cloud	1053:1068	the glycan cloud	1053:1068	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	7	17	theme	antibody-based	1150:1163	arg1	therapy					1165:1171	antibody-based therapy	1150:1171	antibody-based therapy	1150:1171	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	6	18	theme	glycan	710:715	arg1	presence					698:705	presence	698:705	presence of glycan	698:715	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	3	19	theme	vaccine	432:438	arg1	development					440:450	vaccine development	432:450	vaccine development	432:450	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	6	20	theme	binding	781:787	arg1	domain					789:794	the receptor binding domain	768:794	the receptor binding domain	768:794	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	3	21	theme	accessible	363:372	arg1	epitopes					374:381	accessible epitopes	363:381	accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development	363:450	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	6	22	theme	receptor	772:779	arg1	domain					789:794	the receptor binding domain	768:794	the receptor binding domain	768:794	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	0	23	theme	cloud	17:21	arg1	Impact					0:5	Impact	0:5	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.	0:83	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	7	24	theme	B-cell	921:926	arg1	epitopes					928:935	28 B-cell epitopes	918:935	28 B-cell epitopes in the Spike structure and group them	918:973	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	5	25	theme	glycan	589:594	arg1	chains					596:601	The glycan chains	585:601	The glycan chains	585:601	The glycan chains alter the accessible surface areas on the S-protein, impeding antibody-antigen recognition.					
32944295	1	26	theme	pandemic	166:173	arg1	proportions					175:185	pandemic proportions	166:185	pandemic proportions	166:185	The SARS-CoV-2 outbreak originated in China in late 2019 and has since spread to pandemic proportions.					
32944295	0	27	theme	glycan	10:15	arg1	cloud					17:21	glycan cloud	10:21	glycan cloud	10:21	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	3	28	theme	trimeric	261:268	arg1	protein					276:282	the trimeric Spike protein	257:282	the trimeric Spike protein	257:282	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	6	29	theme	S2	863:864	arg1	subunit					866:872	the S2 subunit	859:872	the S2 subunit	859:872	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	1	30	theme	SARS-CoV-2	89:98	arg1	outbreak					100:107	The SARS-CoV-2 outbreak	85:107	The SARS-CoV-2 outbreak	85:107	The SARS-CoV-2 outbreak originated in China in late 2019 and has since spread to pandemic proportions.					
32944295	3	31	theme	antibody-based	387:400	arg1	diagnostics					402:412	antibody-based diagnostics	387:412	antibody-based diagnostics	387:412	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	5	32	from	areas	632:636	arg1	S-protein					645:653	the S-protein	641:653	the S-protein	641:653	The glycan chains alter the accessible surface areas on the S-protein, impeding antibody-antigen recognition.					
32944295	3	33	gly	N-glycosylation	318:332	arg2	sites					334:338	all N-glycosylation sites	314:338	all N-glycosylation sites	314:338	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	7	34	theme	vaccine	1129:1135	arg1	development					1137:1147	vaccine development	1129:1147	vaccine development	1129:1147	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	0	35	theme	epitope	37:43	arg1	prediction					45:54	the B-cell epitope prediction	26:54	the B-cell epitope prediction of SARS-CoV-2 Spike protein	26:82	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	0	36	from	Impact	0:5	arg1	prediction					45:54	the B-cell epitope prediction	26:54	the B-cell epitope prediction of SARS-CoV-2 Spike protein	26:82	Impact of glycan cloud on the B-cell epitope prediction of SARS-CoV-2 Spike protein.					
32944295	7	37	from	epitopes	928:935	arg1	structure					950:958	the Spike structure	940:958	the Spike structure	940:958	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	7	37	from	epitopes	928:935	arg1	group					964:968	group	964:968	group them	964:973	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	4	38	theme	heterogeneous	538:550	arg1	ones					552:555	two heterogeneous ones	534:555	two heterogeneous ones	534:555	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	3	39	theme	Spike	270:274	arg1	protein					276:282	the trimeric Spike protein	257:282	the trimeric Spike protein	257:282	We model the trimeric Spike protein, including flexible loops and all N-glycosylation sites, in order to elucidate accessible epitopes for antibody-based diagnostics, therapeutics and vaccine development.					
32944295	7	40	theme	glycan	998:1003	arg1	cloud					1005:1009	the glycan cloud	994:1009	the glycan cloud	994:1009	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	6	41	theme	S1	734:735	arg1	subunit					737:743	the S1 subunit	730:743	the S1 subunit	730:743	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	7	42	dep	group	964:968	arg1	them					970:973	them	970:973	them	970:973	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	7	43	theme	epitopes	1105:1112	arg1	list					1086:1089	a list	1084:1089	a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics	1084:1187	We identify 28 B-cell epitopes in the Spike structure and group them as non-affected by the glycan cloud versus those which are strongly masked by the glycan cloud, resulting in a list of favourable epitopes as targets for vaccine development, antibody-based therapy and diagnostics.					
32944295	4	44	theme	homogeneous	495:505	arg1	patterns					521:528	six homogeneous glycosylation patterns	491:528	six homogeneous glycosylation patterns	491:528	Based on published experimental data, six homogeneous glycosylation patterns and two heterogeneous ones were used for the analysis.					
32944295	6	45	contain	contains	759:766	arg1	subunit					737:743	the S1 subunit	730:743	the S1 subunit	730:743	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	6	45	contain	contains	759:766	arg2	domain					789:794	the receptor binding domain	768:794	the receptor binding domain	768:794	In presence of glycan, epitopes on the S1 subunit, that notably contains the receptor binding domain, remain mostly accessible to antibodies while those present on the S2 subunit are predominantly inaccessible.					
32944295	1	46	theme	late	132:135	arg1	2019					137:140	late 2019	132:140	late 2019	132:140	The SARS-CoV-2 outbreak originated in China in late 2019 and has since spread to pandemic proportions.					
33713774	7	0	theme	As3+	1191:1194	arg1	random					1228:1233	random	1228:1233	random	1228:1233	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	7	0	theme	As3+	1191:1194	arg1	process					1212:1218	the As3+ ions adsorption process	1187:1218	the As3+ ions adsorption process	1187:1218	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	5	1	from	temperature	812:822	arg1	solutions					917:925	the solutions	913:925	the solutions	913:925	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	6	2	theme	As3+	1037:1040	arg1	adsorption					982:991	92% overall adsorption	970:991	92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+	970:1049	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	5	3	theme	pH	792:793	arg1	value					795:799	pH value	792:799	pH value	792:799	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	2	4	theme	solution	385:392	arg1	presence					358:365	the presence	354:365	the presence of aqueous starch solution in an acidic medium	354:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	4	5	theme	PANI/S/Fe2O3	624:635	arg1	possible					659:666	possible	659:666	possible	659:666	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	5	theme	PANI/S/Fe2O3	624:635	arg1	BC					637:638	PANI/S/Fe2O3 BC	624:638	PANI/S/Fe2O3 BC	624:638	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	8	6	theme	Gram-negative	1371:1383	arg1	bacteria					1385:1392	Gram-negative bacteria	1371:1392	Gram-negative bacteria (Salmonella typhimurium)	1371:1417	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	2	7	from	presence	358:365	arg1	techniques					289:298	the techniques	285:298	the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium	285:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	2	7	from	presence	358:365	arg1	medium					407:412	an acidic medium	397:412	an acidic medium	397:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	2	8	theme	interfacial	324:334	arg1	polymerization					336:349	co-precipitation and interfacial polymerization	303:349	co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium	303:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	4	9	dep	PANI	750:753	arg1	nanoparticles					765:777	nanoparticles	765:777	nanoparticles	765:777	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	8	10	dep	bacteria	1437:1444	arg1	Methicillin-Resistant					1458:1478	Methicillin-Resistant Staphylococcus	1458:1493	Methicillin-Resistant Staphylococcus	1458:1493	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	8	10	dep	bacteria	1437:1444	arg1	isolate					1512:1518	Aureus Clinical isolate	1496:1518	Aureus Clinical isolate	1496:1518	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	8	10	dep	bacteria	1437:1444	arg1	subtilis					1533:1540	Bacillus subtilis	1524:1540	Bacillus subtilis	1524:1540	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	8	10	dep	bacteria	1437:1444	arg1	aureus					1450:1455	S. aureus	1447:1455	S. aureus	1447:1455	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	2	11	theme	co-precipitation	303:318	arg1	polymerization					336:349	co-precipitation and interfacial polymerization	303:349	co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium	303:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	5	12	theme	adsorption	932:941	arg1	process					943:949	the adsorption process	928:949	the adsorption process	928:949	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	4	13	theme	relative	738:745	arg1	As3+					716:719	As3+	716:719	As3+	716:719	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	5	14	theme	adsorbent	874:882	arg1	dose					884:887	adsorbent dose	874:887	adsorbent dose	874:887	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	8	15	theme	antibacterial	1343:1355	arg1	BC					1320:1321	PANI/S/Fe2O3 BC	1307:1321	PANI/S/Fe2O3 BC	1307:1321	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	8	15	theme	antibacterial	1343:1355	arg1	agent					1357:1361	an antibacterial agent	1340:1361	an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis)	1340:1541	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	6	16	theme	adsorption	1056:1065	arg1	equilibrium					1067:1077	the adsorption equilibrium	1052:1077	the adsorption equilibrium	1052:1077	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	2	17	attach	presence	358:365	arg1	medium					407:412	an acidic medium	397:412	an acidic medium	397:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	2	17	attach	presence	358:365	arg2	solution					385:392	aqueous starch solution	370:392	aqueous starch solution	370:392	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	5	18	theme	value	795:799	arg1	terms					783:787	terms	783:787	terms	783:787	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	6	19	theme	overall	1010:1016	arg1	adsorption					1018:1027	100% overall adsorption	1005:1027	100% overall adsorption	1005:1027	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	2	20	theme	ultrasonic	420:429	arg1	irradiation					431:441	ultrasonic irradiation	420:441	ultrasonic irradiation	420:441	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	7	21	theme	adsorption	1142:1151	arg1	analysis					1167:1174	adsorption thermodynamic analysis	1142:1174	adsorption thermodynamic analysis	1142:1174	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	7	22	theme	ions	1196:1199	arg1	random					1228:1233	random	1228:1233	random	1228:1233	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	7	22	theme	ions	1196:1199	arg1	process					1212:1218	the As3+ ions adsorption process	1187:1218	the As3+ ions adsorption process	1187:1218	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	3	23	dep	nano-morphologies	448:464	arg1	The					444:446	The	444:446	The	444:446	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	6	24	theme	overall	974:980	arg1	adsorption					982:991	92% overall adsorption	970:991	92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+	970:1049	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	1	25	theme	new	93:95	arg1	composite					141:149	A new synthesized polyaniline/starch/hematite bio composite	91:149	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC)	91:167	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	25	theme	new	93:95	arg1	material					202:209	an effective material	189:209	an effective material for on-site water remediation	189:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	25	theme	new	93:95	arg1	BC					165:166	PANI/S/Fe2O3 BC	152:166	PANI/S/Fe2O3 BC	152:166	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	26	theme	effective	192:200	arg1	composite					141:149	A new synthesized polyaniline/starch/hematite bio composite	91:149	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC)	91:167	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	26	theme	effective	192:200	arg1	material					202:209	an effective material	189:209	an effective material for on-site water remediation	189:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	5	27	theme	initial	825:831	arg1	concentration					845:857	initial heavy metal concentration	825:857	initial heavy metal concentration	825:857	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	4	28	theme	multiple	606:613	arg1	systems					615:621	single and multiple systems	595:621	single and multiple systems	595:621	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	8	29	theme	Aureus	1496:1501	arg1	isolate					1512:1518	Aureus Clinical isolate	1496:1518	Aureus Clinical isolate	1496:1518	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	1	30	theme	synthesized	97:107	arg1	composite					141:149	A new synthesized polyaniline/starch/hematite bio composite	91:149	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC)	91:167	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	30	theme	synthesized	97:107	arg1	material					202:209	an effective material	189:209	an effective material for on-site water remediation	189:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	30	theme	synthesized	97:107	arg1	BC					165:166	PANI/S/Fe2O3 BC	152:166	PANI/S/Fe2O3 BC	152:166	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	7	31	theme	experimental	1275:1286	arg1	results					1288:1294	experimental results	1275:1294	experimental results	1275:1294	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	6	32	theme	Zn2+	1046:1049	arg1	adsorption					982:991	92% overall adsorption	970:991	92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+	970:1049	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	5	33	theme	contact	860:866	arg1	time					868:871	contact time	860:871	contact time	860:871	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	6	34	theme	%	972:972	arg1	adsorption					982:991	92% overall adsorption	970:991	92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+	970:1049	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	5	35	from	ions	905:908	arg1	solutions					917:925	the solutions	913:925	the solutions	913:925	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	2	36	theme	polymerization	336:349	arg1	techniques					289:298	the techniques	285:298	the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium	285:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	4	37	theme	different	682:690	arg1	Zn2+					722:725	Zn2+	722:725	Zn2+	722:725	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	37	theme	different	682:690	arg1	metals					698:703	different heavy metals	682:703	different heavy metals	682:703	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	37	theme	different	682:690	arg1	As3+					716:719	As3+	716:719	As3+	716:719	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	37	theme	different	682:690	arg1	Co2+					732:735	Co2+	732:735	Co2+	732:735	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	3	38	dep	PANI	562:565	arg1	nanoparticles					577:589	nanoparticles	577:589	nanoparticles	577:589	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	6	39	theme	92	970:971	arg1	%					972:972	%	972:972	%	972:972	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	1	40	theme	bio	137:139	arg1	composite					141:149	A new synthesized polyaniline/starch/hematite bio composite	91:149	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC)	91:167	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	40	theme	bio	137:139	arg1	material					202:209	an effective material	189:209	an effective material for on-site water remediation	189:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	40	theme	bio	137:139	arg1	BC					165:166	PANI/S/Fe2O3 BC	152:166	PANI/S/Fe2O3 BC	152:166	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	9	41	theme	reported	1548:1555	arg1	performances					1557:1568	The reported performances	1544:1568	The reported performances	1544:1568	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	1	42	theme	on-site	215:221	arg1	remediation					229:239	on-site water remediation	215:239	on-site water remediation	215:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	0	43	theme	bio	49:51	arg1	composite					53:61	PANI/starch/Fe2O3 bio composite	31:61	PANI/starch/Fe2O3 bio composite	31:61	Design and characterization of PANI/starch/Fe2O3 bio composite for wastewater remediation.					
33713774	3	44	theme	relative	550:557	arg1	techniques					539:548	various techniques	531:548	various techniques relative to PANI and Fe2O3 nanoparticles	531:589	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	3	45	theme	PANI/S/Fe2O3	497:508	arg1	BC					510:511	the designed PANI/S/Fe2O3 BC	484:511	the designed PANI/S/Fe2O3 BC	484:511	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	9	46	theme	potent	1610:1615	arg1	BC					1602:1603	the PANI/S/Fe2O3 BC	1585:1603	the PANI/S/Fe2O3 BC	1585:1603	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	9	46	theme	potent	1610:1615	arg1	candidate					1617:1625	a potent candidate	1608:1625	a potent candidate for industrial water bioremediation	1608:1661	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	8	47	dep	bacteria	1385:1392	arg1	typhimurium					1406:1416	Salmonella typhimurium	1395:1416	Salmonella typhimurium	1395:1416	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	1	48	theme	water	223:227	arg1	remediation					229:239	on-site water remediation	215:239	on-site water remediation	215:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	0	49	theme	PANI/starch/Fe2O3	31:47	arg1	composite					53:61	PANI/starch/Fe2O3 bio composite	31:61	PANI/starch/Fe2O3 bio composite	31:61	Design and characterization of PANI/starch/Fe2O3 bio composite for wastewater remediation.					
33713774	8	50	dep	Methicillin-Resistant	1458:1478	arg1	Staphylococcus					1480:1493	Methicillin-Resistant Staphylococcus	1458:1493	Methicillin-Resistant Staphylococcus	1458:1493	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	6	51	theme	Co2+	996:999	arg1	adsorption					982:991	92% overall adsorption	970:991	92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+	970:1049	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	5	52	theme	heavy	833:837	arg1	concentration					845:857	initial heavy metal concentration	825:857	initial heavy metal concentration	825:857	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	3	53	theme	BC	510:511	arg1	structures					470:479	structures	470:479	structures	470:479	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	3	53	theme	BC	510:511	arg1	nano-morphologies					448:464	nano-morphologies	448:464	nano-morphologies	448:464	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	5	54	theme	competitive	893:903	arg1	ions					905:908	competitive ions	893:908	competitive ions	893:908	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	5	55	from	time	868:871	arg1	solutions					917:925	the solutions	913:925	the solutions	913:925	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	2	56	theme	starch	378:383	arg1	solution					385:392	aqueous starch solution	370:392	aqueous starch solution	370:392	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	0	57	theme	composite	53:61	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Design and characterization of PANI/starch/Fe2O3 bio composite for wastewater remediation.					
33713774	0	57	theme	composite	53:61	arg1	Design					0:5	Design	0:5	Design	0:5	Design and characterization of PANI/starch/Fe2O3 bio composite for wastewater remediation.					
33713774	4	58	theme	single	595:600	arg1	systems					615:621	single and multiple systems	595:621	single and multiple systems	595:621	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	9	59	theme	industrial	1631:1640	arg1	bioremediation					1648:1661	industrial water bioremediation	1631:1661	industrial water bioremediation	1631:1661	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	5	60	from	concentration	845:857	arg1	solutions					917:925	the solutions	913:925	the solutions	913:925	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	2	61	theme	aqueous	370:376	arg1	solution					385:392	aqueous starch solution	370:392	aqueous starch solution	370:392	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	7	62	theme	thermodynamic	1153:1165	arg1	analysis					1167:1174	adsorption thermodynamic analysis	1142:1174	adsorption thermodynamic analysis	1142:1174	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	9	63	theme	water	1642:1646	arg1	bioremediation					1648:1661	industrial water bioremediation	1631:1661	industrial water bioremediation	1631:1661	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	8	64	theme	Gram-positive	1423:1435	arg1	bacteria					1437:1444	Gram-positive bacteria	1423:1444	Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis)	1423:1541	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	1	65	theme	PANI/S/Fe2O3	152:163	arg1	composite					141:149	A new synthesized polyaniline/starch/hematite bio composite	91:149	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC)	91:167	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	65	theme	PANI/S/Fe2O3	152:163	arg1	BC					165:166	PANI/S/Fe2O3 BC	152:166	PANI/S/Fe2O3 BC	152:166	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	66	theme	polyaniline/starch/hematite	109:135	arg1	composite					141:149	A new synthesized polyaniline/starch/hematite bio composite	91:149	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC)	91:167	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	66	theme	polyaniline/starch/hematite	109:135	arg1	material					202:209	an effective material	189:209	an effective material for on-site water remediation	189:239	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	1	66	theme	polyaniline/starch/hematite	109:135	arg1	BC					165:166	PANI/S/Fe2O3 BC	152:166	PANI/S/Fe2O3 BC	152:166	A new synthesized polyaniline/starch/hematite bio composite (PANI/S/Fe2O3 BC) has been studied as an effective material for on-site water remediation.					
33713774	6	67	theme	adsorption	1018:1027	arg1	adsorption					982:991	92% overall adsorption	970:991	92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+	970:1049	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	8	68	theme	Clinical	1503:1510	arg1	isolate					1512:1518	Aureus Clinical isolate	1496:1518	Aureus Clinical isolate	1496:1518	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	8	69	theme	PANI/S/Fe2O3	1307:1318	arg1	BC					1320:1321	PANI/S/Fe2O3 BC	1307:1321	PANI/S/Fe2O3 BC	1307:1321	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	8	69	theme	PANI/S/Fe2O3	1307:1318	arg1	agent					1357:1361	an antibacterial agent	1340:1361	an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis)	1340:1541	Moreover, PANI/S/Fe2O3 BC was evaluated as an antibacterial agent against Gram-negative bacteria (Salmonella typhimurium) and Gram-positive bacteria (S. aureus, Methicillin-Resistant Staphylococcus, Aureus Clinical isolate and Bacillus subtilis).					
33713774	5	70	from	dose	884:887	arg1	solutions					917:925	the solutions	913:925	the solutions	913:925	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	3	71	theme	various	531:537	arg1	techniques					539:548	various techniques	531:548	various techniques relative to PANI and Fe2O3 nanoparticles	531:589	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	3	72	theme	designed	488:495	arg1	BC					510:511	the designed PANI/S/Fe2O3 BC	484:511	the designed PANI/S/Fe2O3 BC	484:511	The nano-morphologies and structures of the designed PANI/S/Fe2O3 BC were evaluated by various techniques relative to PANI and Fe2O3 nanoparticles.					
33713774	7	73	theme	adsorption	1201:1210	arg1	random					1228:1233	random	1228:1233	random	1228:1233	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	7	73	theme	adsorption	1201:1210	arg1	process					1212:1218	the As3+ ions adsorption process	1187:1218	the As3+ ions adsorption process	1187:1218	In addition, adsorption thermodynamic analysis shows that the As3+ ions adsorption process was not random and the pseudo-second-order fitted with experimental results.					
33713774	4	74	theme	heavy	692:696	arg1	Zn2+					722:725	Zn2+	722:725	Zn2+	722:725	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	74	theme	heavy	692:696	arg1	metals					698:703	different heavy metals	682:703	different heavy metals	682:703	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	74	theme	heavy	692:696	arg1	As3+					716:719	As3+	716:719	As3+	716:719	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	4	74	theme	heavy	692:696	arg1	Co2+					732:735	Co2+	732:735	Co2+	732:735	In single and multiple systems, PANI/S/Fe2O3 BC was evaluated as a possible adsorbent for different heavy metals, including As3+, Zn2+, and Co2+, relative to PANI and Fe2O3 nanoparticles.					
33713774	9	75	theme	PANI/S/Fe2O3	1589:1600	arg1	BC					1602:1603	the PANI/S/Fe2O3 BC	1585:1603	the PANI/S/Fe2O3 BC	1585:1603	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	9	75	theme	PANI/S/Fe2O3	1589:1600	arg1	candidate					1617:1625	a potent candidate	1608:1625	a potent candidate for industrial water bioremediation	1608:1661	The reported performances indicated that the PANI/S/Fe2O3 BC is a potent candidate for industrial water bioremediation.					
33713774	2	76	theme	acidic	400:405	arg1	medium					407:412	an acidic medium	397:412	an acidic medium	397:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	2	77	from	techniques	289:298	arg1	presence					358:365	the presence	354:365	the presence of aqueous starch solution in an acidic medium	354:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	6	78	theme	%	1008:1008	arg1	adsorption					1018:1027	100% overall adsorption	1005:1027	100% overall adsorption	1005:1027	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33713774	0	79	theme	wastewater	67:76	arg1	remediation					78:88	wastewater remediation	67:88	wastewater remediation	67:88	Design and characterization of PANI/starch/Fe2O3 bio composite for wastewater remediation.					
33713774	5	80	theme	metal	839:843	arg1	concentration					845:857	initial heavy metal concentration	825:857	initial heavy metal concentration	825:857	In terms of pH value, operating temperature, initial heavy metal concentration, contact time, adsorbent dose and competitive ions in the solutions, the adsorption process was optimized.					
33713774	2	81	from	polymerization	336:349	arg1	presence					358:365	the presence	354:365	the presence of aqueous starch solution in an acidic medium	354:412	PANI/S/Fe2O3 BC was developed by combining the techniques of co-precipitation and interfacial polymerization in the presence of aqueous starch solution in an acidic medium under ultrasonic irradiation.					
33713774	6	82	theme	100	1005:1007	arg1	%					1008:1008	%	1008:1008	%	1008:1008	For 92% overall adsorption of Co2+ and 100% overall adsorption of both As3+ and Zn2+, the adsorption equilibrium was achieved within 60 and 120 min, respectively.					
33588004	3	0	theme	critical	633:640	arg1	period					642:647	the critical period	629:647	the critical period of reward system development (p21)	629:682	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	2	1	theme	brain	346:350	arg1	system					359:364	The brain reward system	342:364	The brain reward system	342:364	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	1	theme	brain	346:350	arg1	projections					409:419	the mesolimbic dopamine (DA) projections	380:419	specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc)	367:484	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	10	2	theme	system	1801:1806	arg1	development					1808:1818	DA system development	1798:1818	DA system development	1798:1818	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	1	3	theme	infant	249:254	arg1	composition					271:281	infant gut microbiota composition	249:281	infant gut microbiota composition	249:281	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	4	4	theme	SL + 2	921:926	arg1	FL					915:916	2'FL	913:916	2'FL (3'SL + 2'FL)	913:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	4	theme	SL + 2	921:926	arg1	FL					928:929	3'SL + 2'FL	919:929	3'SL + 2'FL	919:929	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	3	5	theme	system	659:664	arg1	development					666:676	reward system development	652:676	reward system development (p21)	652:682	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	3	5	theme	system	659:664	arg1	p21					679:681	p21	679:681	p21	679:681	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	8	6	theme	body	1406:1409	arg1	weight					1411:1416	body weight	1406:1416	body weight	1406:1416	In males, VTA DAT and FosB were negatively correlated with body weight and systemic leptin.					
33588004	10	7	theme	HMO	1669:1671	arg1	supplementation					1673:1687	HMO supplementation	1669:1687	HMO supplementation	1669:1687	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	1	8	theme	key	173:175	arg1	oligosaccharides					143:158	Human milk oligosaccharides	132:158	Human milk oligosaccharides (HMO)s	132:165	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	1	8	theme	key	173:175	arg1	component					177:185	a key component	171:185	a key component in human milk	171:199	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	5	9	dep	VTA	1026:1028	arg1	the					1022:1024	the	1022:1024	the	1022:1024	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	6	10	theme	leptin	1230:1235	arg1	expression					1237:1246	leptin expression	1230:1246	leptin expression in the NAc	1230:1257	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	7	11	theme	VTA	1335:1337	arg1	leptin					1339:1344	VTA leptin	1335:1344	VTA leptin	1335:1344	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	10	12	from	differences	1642:1652	arg1	response					1657:1664	response	1657:1664	response to HMO supplementation	1657:1687	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	4	13	theme	diet	812:815	arg1	combination					889:899	a combination	887:899	a combination of 3'SL	887:907	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	13	theme	diet	812:815	arg1	FL					915:916	2'FL	913:916	2'FL (3'SL + 2'FL)	913:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	13	theme	diet	812:815	arg1	2'-fucosyllactose					858:874	2'-fucosyllactose	858:874	2'-fucosyllactose (2'FL)	858:881	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	13	theme	diet	812:815	arg1	groups					817:822	four fortified diet groups	797:822	four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL)	797:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	13	theme	diet	812:815	arg1	sialyllactose					836:848	3'sialyllactose	834:848	3'sialyllactose (3'FL)	834:855	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	13	theme	diet	812:815	arg1	Control					825:831	Control	825:831	Control	825:831	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	1	14	from	component	177:185	arg1	milk					196:199	human milk	190:199	human milk	190:199	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	7	15	theme	VTA	1317:1319	arg1	ghrelinR					1321:1328	VTA ghrelinR	1317:1328	VTA ghrelinR	1317:1328	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	5	16	theme	western	1042:1048	arg1	blots					1050:1054	western blots	1042:1054	western blots	1042:1054	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	1	17	theme	milk	138:141	arg1	HMO					161:163	HMO	161:163	HMO	161:163	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	1	17	theme	milk	138:141	arg1	component					177:185	a key component	171:185	a key component in human milk	171:199	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	1	17	theme	milk	138:141	arg1	oligosaccharides					143:158	Human milk oligosaccharides	132:158	Human milk oligosaccharides (HMO)s	132:165	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	7	18	theme	D2	1313:1314	arg1	expression					1295:1304	an overall lower expression	1278:1304	an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin	1278:1344	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	5	19	theme	measured	1056:1063	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	19	theme	measured	1056:1063	arg1	FosB					1090:1093	measured the immediate early gene FosB	1056:1093	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	3	20	theme	system	725:730	arg1	structure					701:709	the structure	697:709	the structure of the reward system	697:730	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	4	21	dep	groups	817:822	arg1	combination					889:899	a combination	887:899	a combination of 3'SL	887:907	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	FL					879:880	2'FL	877:880	2'FL	877:880	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	FL					928:929	3'SL + 2'FL	919:929	3'SL + 2'FL	919:929	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	FL					853:854	3'FL	851:854	3'FL	851:854	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	FL					915:916	2'FL	913:916	2'FL (3'SL + 2'FL)	913:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	2'-fucosyllactose					858:874	2'-fucosyllactose	858:874	2'-fucosyllactose (2'FL)	858:881	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	groups					817:822	four fortified diet groups	797:822	four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL)	797:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	sialyllactose					836:848	3'sialyllactose	834:848	3'sialyllactose (3'FL)	834:855	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	21	dep	groups	817:822	arg1	Control					825:831	Control	825:831	Control	825:831	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	3	22	theme	study	570:574	arg1	objective					545:553	The objective	541:553	The objective of the present study	541:574	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	2	23	theme	nucleus	462:468	arg1	accumbens					470:478	nucleus accumbens	462:478	nucleus accumbens (NAc)	462:484	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	23	theme	nucleus	462:468	arg1	NAc					481:483	NAc	481:483	NAc	481:483	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	7	24	theme	lower	1289:1293	arg1	expression					1295:1304	an overall lower expression	1278:1304	an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin	1278:1344	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	10	25	theme	functional	1738:1747	arg1	significance					1749:1760	the functional significance	1734:1760	the functional significance of nutritional interventions during DA system development	1734:1818	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	2	26	from	area	448:451	arg1	system					359:364	The brain reward system	342:364	The brain reward system	342:364	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	26	from	area	448:451	arg1	projections					409:419	the mesolimbic dopamine (DA) projections	380:419	specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc)	367:484	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	27	theme	dopamine	395:402	arg1	system					359:364	The brain reward system	342:364	The brain reward system	342:364	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	27	theme	dopamine	395:402	arg1	projections					409:419	the mesolimbic dopamine (DA) projections	380:419	specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc)	367:484	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	0	28	theme	Mesolimbic	104:113	arg1	System					124:129	the Mesolimbic Dopamine System	100:129	the Mesolimbic Dopamine System	100:129	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	2	29	theme	tegmental	438:446	arg1	VTA					454:456	VTA	454:456	VTA	454:456	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	29	theme	tegmental	438:446	arg1	area					448:451	the ventral tegmental area	426:451	the ventral tegmental area (VTA)	426:457	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	5	30	theme	isoform	1103:1109	arg1	ΔFosB					1111:1115	its isoform ΔFosB	1099:1115	its isoform ΔFosB	1099:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	30	theme	isoform	1103:1109	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	1	31	theme	gut	256:258	arg1	composition					271:281	infant gut microbiota composition	249:281	infant gut microbiota composition	249:281	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	1	32	dep	oligosaccharides	143:158	arg1	s					165:165	s	165:165	Human milk oligosaccharides (HMO)s	132:165	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	5	33	theme	immediate	1069:1077	arg1	gene					1085:1088	the immediate early gene	1065:1088	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	1	34	theme	composition	271:281	arg1	modulator					236:244	an important dietary modulator	215:244	an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis	215:339	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	6	35	from	expression	1237:1246	arg1	NAc					1255:1257	the NAc	1251:1257	the NAc	1251:1257	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	3	36	theme	fortified	600:608	arg1	diets					610:614	HMO fortified diets	596:614	HMO fortified diets given during the critical period of reward system development (p21)	596:682	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	5	37	theme	gene	1085:1088	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	37	theme	gene	1085:1088	arg1	FosB					1090:1093	measured the immediate early gene FosB	1056:1093	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	0	38	theme	Milk	21:24	arg1	Supplementation					42:56	Human Milk Oligosaccharide Supplementation	15:56	Human Milk Oligosaccharide Supplementation During Critical Periods of Development	15:95	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	1	39	theme	associated	287:296	arg1	development					313:323	gut-brain axis development	298:323	gut-brain axis development	298:323	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	6	40	theme	fortified	1146:1154	arg1	diet					1156:1159	the 3'SL + 2'FL fortified diet	1130:1159	the 3'SL + 2'FL fortified diet	1130:1159	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	10	41	theme	further	1706:1712	arg1	investigations					1714:1727	further investigations	1706:1727	further investigations into the functional significance of nutritional interventions during DA system development	1706:1818	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	0	42	theme	Critical	65:72	arg1	Periods					74:80	Critical Periods	65:80	Critical Periods of Development	65:95	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	0	43	theme	Supplementation	42:56	arg1	Effects					4:10	The Effects	0:10	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System	0:129	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	5	44	theme	RNA	943:945	arg1	expression					954:963	Messenger RNA (mRNA) expression	933:963	Messenger RNA (mRNA) expression	933:963	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	9	45	from	differences	1443:1453	arg1	expression					1462:1471	the expression	1458:1471	the expression of DA markers	1458:1485	Sex differences in the expression of DA markers underscore the need to investigate this phenomenon and understand the functional significance in preventing or treating obesity.					
33588004	10	46	theme	DA	1798:1799	arg1	development					1808:1818	DA system development	1798:1818	DA system development	1798:1818	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	5	47	theme	appetite	991:998	arg1	ΔFosB					1111:1115	its isoform ΔFosB	1099:1115	its isoform ΔFosB	1099:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	47	theme	appetite	991:998	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	47	theme	appetite	991:998	arg1	FosB					1090:1093	measured the immediate early gene FosB	1056:1093	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	10	48	theme	interventions	1777:1789	arg1	significance					1749:1760	the functional significance	1734:1760	the functional significance of nutritional interventions during DA system development	1734:1818	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	5	49	theme	Messenger	933:941	arg1	RNA					943:945	Messenger RNA	933:945	Messenger RNA (mRNA) expression	933:963	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	49	theme	Messenger	933:941	arg1	mRNA					948:951	mRNA	948:951	mRNA	948:951	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	3	50	theme	reward	652:657	arg1	development					666:676	reward system development	652:676	reward system development (p21)	652:682	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	3	50	theme	reward	652:657	arg1	p21					679:681	p21	679:681	p21	679:681	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	2	51	theme	reward	352:357	arg1	system					359:364	The brain reward system	342:364	The brain reward system	342:364	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	51	theme	reward	352:357	arg1	projections					409:419	the mesolimbic dopamine (DA) projections	380:419	specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc)	367:484	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	1	52	theme	dietary	228:234	arg1	modulator					236:244	an important dietary modulator	215:244	an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis	215:339	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	6	53	theme	DAT	1185:1187	arg1	expression					1189:1198	DAT expression	1185:1198	DAT expression in the VTA	1185:1209	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	5	54	theme	DA	984:985	arg1	ΔFosB					1111:1115	its isoform ΔFosB	1099:1115	its isoform ΔFosB	1099:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	54	theme	DA	984:985	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	54	theme	DA	984:985	arg1	FosB					1090:1093	measured the immediate early gene FosB	1056:1093	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	10	55	theme	sex	1638:1640	arg1	differences					1642:1652	sex differences	1638:1652	sex differences in response to HMO supplementation	1638:1687	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	2	56	dep	motivation	505:514	arg1	the					501:503	the	501:503	the	501:503	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	4	57	theme	Sprague-Dawley	751:764	arg1	rats					766:769	Sprague-Dawley rats	751:769	Sprague-Dawley rats	751:769	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	1	58	theme	axis	308:311	arg1	development					313:323	gut-brain axis development	298:323	gut-brain axis development	298:323	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	3	59	theme	development	666:676	arg1	period					642:647	the critical period	629:647	the critical period of reward system development (p21)	629:682	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	7	60	theme	leptin	1339:1344	arg1	expression					1295:1304	an overall lower expression	1278:1304	an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin	1278:1344	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	5	61	dep	markers	1011:1017	arg1	ΔFosB					1111:1115	its isoform ΔFosB	1099:1115	its isoform ΔFosB	1099:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	61	dep	markers	1011:1017	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	61	dep	markers	1011:1017	arg1	FosB					1090:1093	measured the immediate early gene FosB	1056:1093	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	8	62	theme	systemic	1422:1429	arg1	leptin					1431:1436	systemic leptin	1422:1436	systemic leptin	1422:1436	In males, VTA DAT and FosB were negatively correlated with body weight and systemic leptin.					
33588004	1	63	theme	human	190:194	arg1	milk					196:199	human milk	190:199	human milk	190:199	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	7	64	theme	ghrelinR	1321:1328	arg1	expression					1295:1304	an overall lower expression	1278:1304	an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin	1278:1344	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	3	65	theme	reward	718:723	arg1	system					725:730	the reward system	714:730	the reward system	714:730	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	5	66	from	markers	1011:1017	arg1	NAc					1034:1036	NAc	1034:1036	NAc	1034:1036	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	66	from	markers	1011:1017	arg1	blots					1050:1054	western blots	1042:1054	western blots	1042:1054	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	66	from	markers	1011:1017	arg1	VTA					1026:1028	VTA	1026:1028	VTA	1026:1028	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	6	67	from	decrease	1173:1180	arg1	expression					1237:1246	leptin expression	1230:1246	leptin expression in the NAc	1230:1257	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	6	67	from	decrease	1173:1180	arg1	expression					1189:1198	DAT expression	1185:1198	DAT expression in the VTA	1185:1209	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	4	68	theme	fortified	802:810	arg1	combination					889:899	a combination	887:899	a combination of 3'SL	887:907	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	68	theme	fortified	802:810	arg1	FL					915:916	2'FL	913:916	2'FL (3'SL + 2'FL)	913:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	68	theme	fortified	802:810	arg1	2'-fucosyllactose					858:874	2'-fucosyllactose	858:874	2'-fucosyllactose (2'FL)	858:881	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	68	theme	fortified	802:810	arg1	groups					817:822	four fortified diet groups	797:822	four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL)	797:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	68	theme	fortified	802:810	arg1	sialyllactose					836:848	3'sialyllactose	834:848	3'sialyllactose (3'FL)	834:855	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	68	theme	fortified	802:810	arg1	Control					825:831	Control	825:831	Control	825:831	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	1	69	theme	Human	132:136	arg1	HMO					161:163	HMO	161:163	HMO	161:163	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	1	69	theme	Human	132:136	arg1	component					177:185	a key component	171:185	a key component in human milk	171:199	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	1	69	theme	Human	132:136	arg1	oligosaccharides					143:158	Human milk oligosaccharides	132:158	Human milk oligosaccharides (HMO)s	132:165	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	7	70	theme	NAc	1309:1311	arg1	D2					1313:1314	NAc D2	1309:1314	NAc D2	1309:1314	Females displayed an overall lower expression of NAc D2, VTA ghrelinR, and VTA leptin.					
33588004	4	71	theme	groups	817:822	arg1	combination					889:899	a combination	887:899	a combination of 3'SL	887:907	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	71	theme	groups	817:822	arg1	FL					915:916	2'FL	913:916	2'FL (3'SL + 2'FL)	913:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	71	theme	groups	817:822	arg1	2'-fucosyllactose					858:874	2'-fucosyllactose	858:874	2'-fucosyllactose (2'FL)	858:881	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	71	theme	groups	817:822	arg1	groups					817:822	four fortified diet groups	797:822	four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL)	797:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	71	theme	groups	817:822	arg1	sialyllactose					836:848	3'sialyllactose	834:848	3'sialyllactose (3'FL)	834:855	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	71	theme	groups	817:822	arg1	one					790:792	one	790:792	one	790:792	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	71	theme	groups	817:822	arg1	Control					825:831	Control	825:831	Control	825:831	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	6	72	theme	SL + 2	1136:1141	arg1	diet					1156:1159	the 3'SL + 2'FL fortified diet	1130:1159	the 3'SL + 2'FL fortified diet	1130:1159	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	3	73	theme	present	562:568	arg1	study					570:574	the present study	558:574	the present study	558:574	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	9	74	theme	Sex	1439:1441	arg1	differences					1443:1453	Sex differences	1439:1453	Sex differences in the expression of DA markers	1439:1485	Sex differences in the expression of DA markers underscore the need to investigate this phenomenon and understand the functional significance in preventing or treating obesity.					
33588004	0	75	theme	Development	85:95	arg1	Periods					74:80	Critical Periods	65:80	Critical Periods of Development	65:95	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	0	76	from	Effects	4:10	arg1	System					124:129	the Mesolimbic Dopamine System	100:129	the Mesolimbic Dopamine System	100:129	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	0	77	theme	Dopamine	115:122	arg1	System					124:129	the Mesolimbic Dopamine System	100:129	the Mesolimbic Dopamine System	100:129	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	9	78	theme	DA	1476:1477	arg1	markers					1479:1485	DA markers	1476:1485	DA markers	1476:1485	Sex differences in the expression of DA markers underscore the need to investigate this phenomenon and understand the functional significance in preventing or treating obesity.					
33588004	2	79	theme	mesolimbic	384:393	arg1	DA					405:406	DA	405:406	DA	405:406	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	79	theme	mesolimbic	384:393	arg1	dopamine					395:402	mesolimbic dopamine	384:402	specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc)	367:484	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	10	80	from	need	1697:1700	arg1	response					1657:1664	response	1657:1664	response to HMO supplementation	1657:1687	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	2	81	theme	ventral	430:436	arg1	VTA					454:456	VTA	454:456	VTA	454:456	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	2	81	theme	ventral	430:436	arg1	area					448:451	the ventral tegmental area	426:451	the ventral tegmental area (VTA)	426:457	The brain reward system, specifically the mesolimbic dopamine (DA) projections from the ventral tegmental area (VTA) to nucleus accumbens (NAc) is involved in the motivation and preference for food.					
33588004	0	82	theme	Human	15:19	arg1	Supplementation					42:56	Human Milk Oligosaccharide Supplementation	15:56	Human Milk Oligosaccharide Supplementation During Critical Periods of Development	15:95	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	1	83	theme	microbiota	260:269	arg1	composition					271:281	infant gut microbiota composition	249:281	infant gut microbiota composition	249:281	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	3	84	theme	HMO	596:598	arg1	diets					610:614	HMO fortified diets	596:614	HMO fortified diets given during the critical period of reward system development (p21)	596:682	The objective of the present study was to determine if HMO fortified diets given during the critical period of reward system development (p21) could affect the structure of the reward system.					
33588004	5	85	theme	early	1079:1083	arg1	gene					1085:1088	the immediate early gene	1065:1088	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	0	86	theme	Oligosaccharide	26:40	arg1	Supplementation					42:56	Human Milk Oligosaccharide Supplementation	15:56	Human Milk Oligosaccharide Supplementation During Critical Periods of Development	15:95	The Effects of Human Milk Oligosaccharide Supplementation During Critical Periods of Development on the Mesolimbic Dopamine System.					
33588004	10	87	theme	nutritional	1765:1775	arg1	interventions					1777:1789	nutritional interventions	1765:1789	nutritional interventions	1765:1789	This study highlights sex differences in response to HMO supplementation and the need for further investigations into the functional significance of nutritional interventions during DA system development.					
33588004	6	88	theme	FL	1143:1144	arg1	diet					1156:1159	the 3'SL + 2'FL fortified diet	1130:1159	the 3'SL + 2'FL fortified diet	1130:1159	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	1	89	theme	gut-brain	298:306	arg1	development					313:323	gut-brain axis development	298:323	gut-brain axis development	298:323	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	6	90	from	increase	1218:1225	arg1	expression					1237:1246	leptin expression	1230:1246	leptin expression in the NAc	1230:1257	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	6	90	from	increase	1218:1225	arg1	expression					1189:1198	DAT expression	1185:1198	DAT expression in the VTA	1185:1209	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	1	91	theme	development	313:323	arg1	modulator					236:244	an important dietary modulator	215:244	an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis	215:339	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
33588004	6	92	from	expression	1189:1198	arg1	VTA					1207:1209	the VTA	1203:1209	the VTA	1203:1209	Females fed the 3'SL + 2'FL fortified diet displayed a decrease in DAT expression in the VTA and an increase in leptin expression in the NAc.					
33588004	4	93	theme	SL	906:907	arg1	combination					889:899	a combination	887:899	a combination of 3'SL	887:907	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	FL					879:880	2'FL	877:880	2'FL	877:880	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	FL					928:929	3'SL + 2'FL	919:929	3'SL + 2'FL	919:929	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	FL					853:854	3'FL	851:854	3'FL	851:854	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	FL					915:916	2'FL	913:916	2'FL (3'SL + 2'FL)	913:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	2'-fucosyllactose					858:874	2'-fucosyllactose	858:874	2'-fucosyllactose (2'FL)	858:881	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	groups					817:822	four fortified diet groups	797:822	four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL)	797:930	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	sialyllactose					836:848	3'sialyllactose	834:848	3'sialyllactose (3'FL)	834:855	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	4	93	theme	SL	906:907	arg1	Control					825:831	Control	825:831	Control	825:831	At weaning (p21), Sprague-Dawley rats were randomized to one of four fortified diet groups: Control, 3'sialyllactose (3'FL), 2'-fucosyllactose (2'FL), or a combination of 3'SL and 2'FL (3'SL + 2'FL).					
33588004	8	94	theme	VTA	1357:1359	arg1	DAT					1361:1363	VTA DAT	1357:1363	VTA DAT	1357:1363	In males, VTA DAT and FosB were negatively correlated with body weight and systemic leptin.					
33588004	5	95	theme	associated	1000:1009	arg1	ΔFosB					1111:1115	its isoform ΔFosB	1099:1115	its isoform ΔFosB	1099:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	95	theme	associated	1000:1009	arg1	markers					1011:1017	DA and appetite associated markers	984:1017	DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB	984:1115	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	5	95	theme	associated	1000:1009	arg1	FosB					1090:1093	measured the immediate early gene FosB	1056:1093	measured the immediate early gene FosB	1056:1093	Messenger RNA (mRNA) expression was quantified for DA and appetite associated markers in the VTA and NAc and western blots measured the immediate early gene FosB and its isoform ΔFosB.					
33588004	9	96	theme	functional	1557:1566	arg1	significance					1568:1579	the functional significance	1553:1579	the functional significance in preventing or treating obesity	1553:1613	Sex differences in the expression of DA markers underscore the need to investigate this phenomenon and understand the functional significance in preventing or treating obesity.					
33588004	9	97	theme	markers	1479:1485	arg1	expression					1462:1471	the expression	1458:1471	the expression of DA markers	1458:1485	Sex differences in the expression of DA markers underscore the need to investigate this phenomenon and understand the functional significance in preventing or treating obesity.					
33588004	1	98	theme	important	218:226	arg1	modulator					236:244	an important dietary modulator	215:244	an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis	215:339	Human milk oligosaccharides (HMO)s are a key component in human milk and represent an important dietary modulator of infant gut microbiota composition and associated gut-brain axis development and homeostasis.					
35014483	9	0	theme	wt	1403:1404	arg1	blends					1421:1426	1 wt % chitosan PCL blends	1401:1426	1 wt % chitosan PCL blends	1401:1426	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	8	1	theme	three-dimensional	1267:1283	arg1	structure					1319:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	4	2	theme	chitosan	698:705	arg1	scaffolds					711:719	chitosan MEW scaffolds	698:719	chitosan MEW scaffolds	698:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	1	3	theme	microfibrous	191:202	arg1	scaffolds					204:212	microfibrous scaffolds	191:212	microfibrous scaffolds	191:212	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	10	4	theme	PCL/chitosan	1576:1587	arg1	scaffolds					1599:1607	melt-electrowritten PCL/chitosan composite scaffolds	1556:1607	melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture	1556:1641	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	6	5	theme	TECs	1016:1019	arg1	characterizations					975:991	In vitro physical and biological characterizations	942:991	In vitro physical and biological characterizations of melt-electrowritten TECs	942:1019	In vitro physical and biological characterizations of melt-electrowritten TECs were performed.					
35014483	5	6	theme	stem	921:924	arg1	hBMSCs					933:938	hBMSCs	933:938	hBMSCs	933:938	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	6	theme	stem	921:924	arg1	cells					926:930	human bone-marrow-derived mesenchymal stem cells	883:930	human bone-marrow-derived mesenchymal stem cells (hBMSCs)	883:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	7	theme	human	883:887	arg1	hBMSCs					933:938	hBMSCs	933:938	hBMSCs	933:938	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	7	theme	human	883:887	arg1	cells					926:930	human bone-marrow-derived mesenchymal stem cells	883:930	human bone-marrow-derived mesenchymal stem cells (hBMSCs)	883:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	2	8	theme	due	384:386	arg1	Polycaprolactone					301:316	Polycaprolactone	301:316	Polycaprolactone (PCL)	301:322	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	2	8	theme	due	384:386	arg1	material					375:382	the scaffold material	362:382	the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability	362:474	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	6	9	theme	melt-electrowritten	996:1014	arg1	TECs					1016:1019	melt-electrowritten TECs	996:1019	melt-electrowritten TECs	996:1019	In vitro physical and biological characterizations of melt-electrowritten TECs were performed.					
35014483	10	10	theme	potential	1653:1661	arg1	use					1663:1665	their potential use	1647:1665	their potential use for regenerative, tissue engineering applications	1647:1715	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	3	11	theme	fibrous	562:568	arg1	scaffolds					580:588	MEW fibrous composite scaffolds	558:588	MEW fibrous composite scaffolds	558:588	To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds.					
35014483	10	12	theme	regenerative	1671:1682	arg1	applications					1704:1715	regenerative, tissue engineering applications	1671:1715	regenerative, tissue engineering applications	1671:1715	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	3	13	theme	composite	570:578	arg1	scaffolds					580:588	MEW fibrous composite scaffolds	558:588	MEW fibrous composite scaffolds	558:588	To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds.					
35014483	9	14	from	increase	1358:1365	arg1	comparison					1431:1440	comparison	1431:1440	comparison to PCL-only scaffolds	1431:1462	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	9	14	from	increase	1358:1365	arg1	blends					1421:1426	1 wt % chitosan PCL blends	1401:1426	1 wt % chitosan PCL blends	1401:1426	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	9	14	from	increase	1358:1365	arg1	proliferation					1384:1396	hBMSC proliferation	1378:1396	hBMSC proliferation	1378:1396	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	8	15	theme	scaffold	1310:1317	arg1	structure					1319:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	1	16	theme	Melt	114:117	arg1	technique					155:163	an emerging technique	143:163	an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required	143:298	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	1	16	theme	Melt	114:117	arg1	MEW					135:137	MEW	135:137	MEW	135:137	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	1	16	theme	Melt	114:117	arg1	electrowriting					119:132	Melt electrowriting	114:132	Melt electrowriting (MEW)	114:138	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	5	17	theme	cells	926:930	arg1	growth					873:878	the growth	869:878	the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs)	869:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	2	18	theme	mechanical	431:440	arg1	strength					442:449	mechanical strength	431:449	mechanical strength	431:449	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	5	19	theme	study	738:742	arg1	aim					726:728	The aim	722:728	The aim of this study	722:742	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	19	theme	study	738:742	arg1	melt					751:754	melt	751:754	melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs)	751:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	9	20	theme	PCL-only	1445:1452	arg1	scaffolds					1454:1462	PCL-only scaffolds	1445:1462	PCL-only scaffolds	1445:1462	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	3	21	theme	MEW	558:560	arg1	scaffolds					580:588	MEW fibrous composite scaffolds	558:588	MEW fibrous composite scaffolds	558:588	To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds.					
35014483	1	22	theme	ideal	215:219	arg1	scaffolds					204:212	microfibrous scaffolds	191:212	microfibrous scaffolds	191:212	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	0	23	theme	Melt-Electrowritten	18:36	arg1	Scaffolds					64:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds	0:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds	0:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior.					
35014483	10	24	theme	composite	1589:1597	arg1	scaffolds					1599:1607	melt-electrowritten PCL/chitosan composite scaffolds	1556:1607	melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture	1556:1641	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	9	25	theme	hBMSC	1378:1382	arg1	proliferation					1384:1396	hBMSC proliferation	1378:1396	hBMSC proliferation	1378:1396	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	7	26	theme	reproducible	1075:1086	arg1	scaffolds					1110:1118	reproducible, layered micron-range scaffolds	1075:1118	reproducible, layered micron-range scaffolds	1075:1118	Physical characterization showed that reproducible, layered micron-range scaffolds could be successfully fabricated.					
35014483	4	27	theme	behavior	671:678	arg1	effects					650:656	the effects	646:656	the effects of stem cell behavior on PCL containing chitosan MEW scaffolds	646:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	0	28	theme	Three-Dimensional	0:16	arg1	Scaffolds					64:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds	0:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds	0:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior.					
35014483	6	29	theme	biological	964:973	arg1	characterizations					975:991	In vitro physical and biological characterizations	942:991	In vitro physical and biological characterizations of melt-electrowritten TECs	942:1019	In vitro physical and biological characterizations of melt-electrowritten TECs were performed.					
35014483	8	30	theme	3D	1286:1287	arg1	structure					1319:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	4	31	from	effects	650:656	arg1	PCL					683:685	PCL	683:685	PCL containing chitosan MEW scaffolds	683:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	1	32	theme	tissue	225:230	arg1	engineering					232:242	tissue engineering	225:242	tissue engineering	225:242	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	8	33	theme	cell	1234:1237	arg1	infiltration					1239:1250	cell infiltration	1234:1250	cell infiltration	1234:1250	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	5	34	theme	constructs	811:820	arg1	range					771:775	a range	769:775	a range of PCL/chitosan tissue-engineered constructs (TECs)	769:827	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	35	dep	melt	751:754	arg1	assess					833:838	assess	833:838	assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs)	833:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	35	dep	melt	751:754	arg1	electrowrite					756:767	electrowrite	756:767	electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs)	756:827	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	10	36	with	scaffolds	1599:1607	arg1	microarchitecture					1625:1641	controlled microarchitecture	1614:1641	controlled microarchitecture	1614:1641	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	2	37	theme	melt	456:459	arg1	processability					461:474	melt processability	456:474	melt processability	456:474	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	0	38	theme	Polycaprolactone/Chitosan	38:62	arg1	Scaffolds					64:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds	0:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds	0:72	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior.					
35014483	6	39	theme	physical	951:958	arg1	characterizations					975:991	In vitro physical and biological characterizations	942:991	In vitro physical and biological characterizations of melt-electrowritten TECs	942:1019	In vitro physical and biological characterizations of melt-electrowritten TECs were performed.					
35014483	5	40	link	bone-marrow-derived	889:907	arg1	hBMSCs					933:938	hBMSCs	933:938	hBMSCs	933:938	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	40	link	bone-marrow-derived	889:907	arg1	cells					926:930	human bone-marrow-derived mesenchymal stem cells	883:930	human bone-marrow-derived mesenchymal stem cells (hBMSCs)	883:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	41	theme	bone-marrow-derived	889:907	arg1	hBMSCs					933:938	hBMSCs	933:938	hBMSCs	933:938	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	41	theme	bone-marrow-derived	889:907	arg1	cells					926:930	human bone-marrow-derived mesenchymal stem cells	883:930	human bone-marrow-derived mesenchymal stem cells (hBMSCs)	883:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	4	42	theme	kind	631:634	arg1	study					618:622	the first study	608:622	the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds	608:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	4	42	theme	kind	631:634	arg1	this					600:603	this	600:603	this	600:603	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	0	43	theme	Mesenchymal	82:92	arg1	Behavior					104:111	Mesenchymal Stem Cell Behavior	82:111	Mesenchymal Stem Cell Behavior	82:111	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior.					
35014483	2	44	theme	scaffold	366:373	arg1	Polycaprolactone					301:316	Polycaprolactone	301:316	Polycaprolactone (PCL)	301:322	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	2	44	theme	scaffold	366:373	arg1	material					375:382	the scaffold material	362:382	the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability	362:474	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	4	45	theme	cell	666:669	arg1	behavior					671:678	stem cell behavior	661:678	stem cell behavior	661:678	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	3	46	theme	PCL	489:491	arg1	bioactivity					493:503	PCL bioactivity	489:503	PCL bioactivity	489:503	To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds.					
35014483	4	47	theme	stem	661:664	arg1	behavior					671:678	stem cell behavior	661:678	stem cell behavior	661:678	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	6	48	theme	In	942:943	arg1	characterizations					975:991	In vitro physical and biological characterizations	942:991	In vitro physical and biological characterizations of melt-electrowritten TECs	942:1019	In vitro physical and biological characterizations of melt-electrowritten TECs were performed.					
35014483	6	49	dep	In	942:943	arg1	vitro					945:949	vitro	945:949	vitro	945:949	In vitro physical and biological characterizations of melt-electrowritten TECs were performed.					
35014483	10	50	dep	regenerative	1671:1682	arg1	engineering					1692:1702	engineering	1692:1702	engineering	1692:1702	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	1	51	theme	biomimetic	251:260	arg1	detail					281:286	biomimetic microarchitectural detail	251:286	biomimetic microarchitectural detail	251:286	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	0	52	theme	Cell	99:102	arg1	Behavior					104:111	Mesenchymal Stem Cell Behavior	82:111	Mesenchymal Stem Cell Behavior	82:111	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior.					
35014483	9	53	theme	%	1406:1406	arg1	PCL					1417:1419	% chitosan PCL	1406:1419	1 wt % chitosan PCL blends	1401:1426	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	5	54	theme	PCL/chitosan	780:791	arg1	constructs					811:820	PCL/chitosan tissue-engineered constructs	780:820	PCL/chitosan tissue-engineered constructs (TECs)	780:827	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	54	theme	PCL/chitosan	780:791	arg1	TECs					823:826	TECs	823:826	TECs	823:826	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	7	55	theme	Physical	1037:1044	arg1	characterization					1046:1061	Physical characterization	1037:1061	Physical characterization	1037:1061	Physical characterization showed that reproducible, layered micron-range scaffolds could be successfully fabricated.					
35014483	5	56	theme	mesenchymal	909:919	arg1	hBMSCs					933:938	hBMSCs	933:938	hBMSCs	933:938	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	56	theme	mesenchymal	909:919	arg1	cells					926:930	human bone-marrow-derived mesenchymal stem cells	883:930	human bone-marrow-derived mesenchymal stem cells (hBMSCs)	883:939	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	0	57	theme	Stem	94:97	arg1	Behavior					104:111	Mesenchymal Stem Cell Behavior	82:111	Mesenchymal Stem Cell Behavior	82:111	Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior.					
35014483	9	58	theme	chitosan	1408:1415	arg1	PCL					1417:1419	% chitosan PCL	1406:1419	1 wt % chitosan PCL blends	1401:1426	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	7	59	theme	micron-range	1097:1108	arg1	scaffolds					1110:1118	reproducible, layered micron-range scaffolds	1075:1118	reproducible, layered micron-range scaffolds	1075:1118	Physical characterization showed that reproducible, layered micron-range scaffolds could be successfully fabricated.					
35014483	10	60	theme	scaffolds	1599:1607	arg1	use					1663:1665	their potential use	1647:1665	their potential use for regenerative, tissue engineering applications	1647:1715	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	10	60	theme	scaffolds	1599:1607	arg1	fabrication					1541:1551	the fabrication	1537:1551	the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture	1537:1641	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	9	61	theme	significant	1346:1356	arg1	increase					1358:1365	A statistically significant increase	1330:1365	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds	1330:1462	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	9	61	theme	significant	1346:1356	arg1	%					1372:1372	∼140%	1368:1372	∼140%	1368:1372	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	1	62	theme	emerging	146:153	arg1	technique					155:163	an emerging technique	143:163	an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required	143:298	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	1	62	theme	emerging	146:153	arg1	electrowriting					119:132	Melt electrowriting	114:132	Melt electrowriting (MEW)	114:138	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	9	63	theme	PCL	1417:1419	arg1	blends					1421:1426	1 wt % chitosan PCL blends	1401:1426	1 wt % chitosan PCL blends	1401:1426	A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks.					
35014483	8	64	theme	melt-electrowritten	1290:1308	arg1	structure					1319:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	the three-dimensional (3D) melt-electrowritten scaffold structure	1263:1327	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	3	65	theme	natural	508:514	arg1	chitosan					525:532	chitosan	525:532	chitosan	525:532	To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds.					
35014483	3	65	theme	natural	508:514	arg1	polymer					516:522	a natural polymer	506:522	a natural polymer	506:522	To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds.					
35014483	1	66	theme	microarchitectural	262:279	arg1	detail					281:286	biomimetic microarchitectural detail	251:286	biomimetic microarchitectural detail	251:286	Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required.					
35014483	2	67	theme	synthetic	327:335	arg1	Polycaprolactone					301:316	Polycaprolactone	301:316	Polycaprolactone (PCL)	301:322	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	2	67	theme	synthetic	327:335	arg1	polymer					337:343	a synthetic polymer	325:343	a synthetic polymer	325:343	Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability.					
35014483	7	68	dep	reproducible	1075:1086	arg1	layered					1089:1095	layered	1089:1095	layered	1089:1095	Physical characterization showed that reproducible, layered micron-range scaffolds could be successfully fabricated.					
35014483	4	69	theme	first	612:616	arg1	study					618:622	the first study	608:622	the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds	608:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	4	69	theme	first	612:616	arg1	this					600:603	this	600:603	this	600:603	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	10	70	theme	controlled	1614:1623	arg1	microarchitecture					1625:1641	controlled microarchitecture	1614:1641	controlled microarchitecture	1614:1641	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	10	71	theme	melt-electrowritten	1556:1574	arg1	scaffolds					1599:1607	melt-electrowritten PCL/chitosan composite scaffolds	1556:1607	melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture	1556:1641	Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.					
35014483	8	72	theme	cell	1163:1166	arg1	proliferation					1182:1194	proliferation	1182:1194	proliferation	1182:1194	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	8	72	theme	cell	1163:1166	arg1	well					1157:1160	well	1157:1160	well	1157:1160	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	8	72	theme	cell	1163:1166	arg1	migration					1168:1176	cell migration	1163:1176	cell migration	1163:1176	As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure.					
35014483	4	73	contain	containing	687:696	arg2	scaffolds					711:719	chitosan MEW scaffolds	698:719	chitosan MEW scaffolds	698:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	4	73	contain	containing	687:696	arg1	PCL					683:685	PCL	683:685	PCL containing chitosan MEW scaffolds	683:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
35014483	5	74	theme	tissue-engineered	793:809	arg1	constructs					811:820	PCL/chitosan tissue-engineered constructs	780:820	PCL/chitosan tissue-engineered constructs (TECs)	780:827	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	5	74	theme	tissue-engineered	793:809	arg1	TECs					823:826	TECs	823:826	TECs	823:826	The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs).					
35014483	4	75	theme	MEW	707:709	arg1	scaffolds					711:719	chitosan MEW scaffolds	698:719	chitosan MEW scaffolds	698:719	To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds.					
32522274	5	0	dep	stability	1375:1383	arg1	properties					1505:1514	enforced mechanical properties	1485:1514	enforced mechanical properties	1485:1514	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	1	dep	%	958:958	arg1	168.31 kPa					961:970	168.31 kPa	961:970	168.31 kPa	961:970	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	1	dep	%	958:958	arg1	%					977:977	0.04%	973:977	0.04%	973:977	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	1	dep	%	958:958	arg1	449.59 kPa					980:989	449.59 kPa	980:989	449.59 kPa	980:989	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	3	2	theme	nanocomposite	567:579	arg1	hydrogels					581:589	These HA nanocomposite hydrogels	558:589	These HA nanocomposite hydrogels loaded with surface functionalized ND	558:627	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	5	3	theme	layer-based	1439:1449	arg1	deposition					1451:1460	layer-based deposition	1439:1460	layer-based deposition at microscale attained	1439:1483	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	1	4	theme	biomedical	220:229	arg1	applications					231:242	biomedical applications	220:242	biomedical applications	220:242	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	3	5	theme	surface	603:609	arg1	ND					626:627	surface functionalized ND	603:627	surface functionalized ND	603:627	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	4	6	theme	0.04 wt	901:907	arg1	composition					873:883	the composition	869:883	the composition of 0.02 wt% and 0.04 wt	869:907	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	7	theme	compressive	1022:1032	arg1	stress					1034:1039	the compressive stress	1018:1039	the compressive stress of HA/ND-OH (0.04 wt%) at pH 8	1018:1070	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	1	8	theme	mechanical	265:274	arg1	properties					276:285	their desired mechanical properties	251:285	their desired mechanical properties	251:285	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	5	9	theme	mechanical	1494:1503	arg1	properties					1505:1514	enforced mechanical properties	1485:1514	enforced mechanical properties	1485:1514	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	10	dep	1.37-fold	813:821	arg1	%					828:828	0.04%	824:828	0.04%: 616.72 kPa	824:840	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	6	11	theme	ND	1566:1567	arg1	nanophase					1569:1577	the inorganic ND nanophase	1552:1577	the inorganic ND nanophase	1552:1577	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	5	12	with	interaction	1224:1234	arg1	chains					1250:1255	the long chains	1241:1255	the long chains of HA at the molecular level	1241:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	13	theme	%	895:895	arg1	composition					873:883	the composition	869:883	the composition of 0.02 wt% and 0.04 wt	869:907	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	2	14	theme	nanocomposite	323:335	arg1	hydrogels					337:345	3D printing nanocomposite hydrogels	311:345	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND)	311:386	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	5	15	with	environment	1208:1218	arg1	chains					1250:1255	the long chains	1241:1255	the long chains of HA at the molecular level	1241:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	2	16	theme	printing	314:321	arg1	hydrogels					337:345	3D printing nanocomposite hydrogels	311:345	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND)	311:386	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	17	from	pH.	1004:1006	arg1	ND-COOH					945:951	ND-COOH	945:951	ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7	945:1155	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	17	from	pH.	1004:1006	arg1	%					958:958	0.02%	954:958	0.02%	954:958	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	18	theme	nanocomposite	714:726	arg1	hydrogels					728:736	HA nanocomposite hydrogels	711:736	HA nanocomposite hydrogels with ND-OH at pH 8	711:755	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	0	19	theme	hyaluronic	74:83	arg1	hydrogels					104:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	3	20	theme	HA	564:565	arg1	hydrogels					581:589	These HA nanocomposite hydrogels	558:589	These HA nanocomposite hydrogels loaded with surface functionalized ND	558:627	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	3	21	theme	enforced	645:652	arg1	stress					666:671	the enforced compressive stress	641:671	the enforced compressive stress	641:671	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	0	22	theme	nanocomposite	90:102	arg1	hydrogels					104:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	6	23	theme	surface	1534:1540	arg1	tuning					1542:1547	Fine surface tuning	1529:1547	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing	1529:1604	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	4	24	dep	1.40-fold	779:787	arg1	stress					859:864	the compressive stress	843:864	the compressive stress	843:864	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	24	dep	1.40-fold	779:787	arg1	%					794:794	0.02%	790:794	0.02%: 236.18 kPa	790:806	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	6	25	theme	improved	1615:1622	arg1	control					1624:1630	improved control	1615:1630	improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein	1615:1721	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	2	26	theme	hyaluronic	350:359	arg1	HA					367:368	HA	367:368	HA	367:368	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	2	26	theme	hyaluronic	350:359	arg1	acid					361:364	hyaluronic acid	350:364	hyaluronic acid (HA)/nanodiamond (ND)	350:386	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	2	27	theme	HA	534:535	arg1	nanocomposites					542:555	HA only nanocomposites	534:555	HA only nanocomposites	534:555	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	28	theme	0.04 wt	1139:1145	arg1	HA/ND-OH					1129:1136	HA/ND-OH	1129:1136	HA/ND-OH (0.04 wt%) at pH 7	1129:1155	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	28	theme	0.04 wt	1139:1145	arg1	%					1146:1146	0.04 wt%	1139:1146	0.04 wt%	1139:1146	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	2	29	theme	low	430:432	arg1	ratio					441:445	the low weight ratio	426:445	the low weight ratio of 0.02 wt%	426:457	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	2	30	theme	higher	471:476	arg1	force					490:494	higher compressive force	471:494	higher compressive force	471:494	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	6	31	theme	3D	1594:1595	arg1	printing					1597:1604	controlled 3D printing	1583:1604	controlled 3D printing	1583:1604	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	5	32	theme	mechanical	1332:1341	arg1	properties					1343:1352	the mechanical properties	1328:1352	the mechanical properties	1328:1352	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	0	33	theme	pH-dependent	0:11	arg1	nanodiamonds					13:24	pH-dependent nanodiamonds	0:24	pH-dependent nanodiamonds	0:24	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	1	34	theme	Nanocomposite	115:127	arg1	hydrogels					129:137	Nanocomposite hydrogels	115:137	Nanocomposite hydrogels capable of undergoing manufacturing process	115:181	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	6	35	theme	hydrogels	1697:1705	arg1	properties					1665:1674	the pH-dependent mechanical properties	1637:1674	the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein	1637:1721	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	2	36	theme	0.02 wt	450:456	arg1	%					457:457	0.02 wt%	450:457	0.02 wt%	450:457	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	1	37	theme	capable	139:145	arg1	hydrogels					129:137	Nanocomposite hydrogels	115:137	Nanocomposite hydrogels capable of undergoing manufacturing process	115:181	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	2	38	theme	gel	500:502	arg1	point					513:517	gel breaking point	500:517	gel breaking point	500:517	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	5	39	theme	nanophase	1398:1406	arg1	stability					1375:1383	the pH stability	1368:1383	the pH stability of the ND-OH nanophase	1368:1406	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	39	theme	nanophase	1398:1406	arg1	processing					1424:1433	filament-based processing	1409:1433	filament-based processing	1409:1433	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	39	theme	nanophase	1398:1406	arg1	deposition					1451:1460	layer-based deposition	1439:1460	layer-based deposition at microscale attained	1439:1483	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	40	theme	buffering	1198:1206	arg1	environment					1208:1218	the tunable buffering environment	1186:1218	the tunable buffering environment	1186:1218	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	41	contain	have	1286:1289	arg1	interaction					1224:1234	interaction	1224:1234	interaction with the long chains of HA at the molecular level	1224:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	41	contain	have	1286:1289	arg2	role					1302:1305	a critical role	1291:1305	a critical role	1291:1305	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	41	contain	have	1286:1289	arg1	environment					1208:1218	the tunable buffering environment	1186:1218	the tunable buffering environment	1186:1218	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	0	42	theme	mechanical	38:47	arg1	properties					49:58	the mechanical properties	34:58	the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels	34:112	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	6	43	theme	pH-dependent	1641:1652	arg1	properties					1665:1674	the pH-dependent mechanical properties	1637:1674	the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein	1637:1721	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	5	44	theme	molecular	1270:1278	arg1	level					1280:1284	the molecular level	1266:1284	the molecular level	1266:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	45	from	pH	752:753	arg1	hydrogels					728:736	HA nanocomposite hydrogels	711:736	HA nanocomposite hydrogels with ND-OH at pH 8	711:755	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	3	46	theme	pH-dependent	690:701	arg1	manner					703:708	a pH-dependent manner	688:708	a pH-dependent manner	688:708	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	5	47	theme	long	1245:1248	arg1	chains					1250:1255	the long chains	1241:1255	the long chains of HA at the molecular level	1241:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	1	48	theme	high	291:294	arg1	functionality					296:308	high functionality	291:308	high functionality	291:308	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	5	49	from	dependency	1314:1323	arg1	pH.					1357:1359	pH.	1357:1359	pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel	1357:1526	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	50	theme	microscale	1465:1474	arg1	attained					1476:1483	microscale attained	1465:1483	microscale attained	1465:1483	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	51	theme	compressive	847:857	arg1	stress					859:864	the compressive stress	843:864	the compressive stress	843:864	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	5	52	theme	filament-based	1409:1422	arg1	processing					1424:1433	filament-based processing	1409:1433	filament-based processing	1409:1433	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	2	53	theme	acid	361:364	arg1	ND					384:385	ND	384:385	ND	384:385	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	2	53	theme	acid	361:364	arg1	/nanodiamond					370:381	hyaluronic acid (HA)/nanodiamond	350:381	hyaluronic acid (HA)/nanodiamond (ND)	350:386	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	54	theme	0.04 wt	1054:1060	arg1	HA/ND-OH					1044:1051	HA/ND-OH	1044:1051	HA/ND-OH (0.04 wt%)	1044:1062	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	54	theme	0.04 wt	1054:1060	arg1	%					1061:1061	0.04 wt%	1054:1061	0.04 wt%	1054:1061	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	55	with	hydrogels	728:736	arg1	ND-OH					743:747	ND-OH	743:747	ND-OH	743:747	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	5	56	theme	hydrogel	1519:1526	arg1	stability					1375:1383	the pH stability	1368:1383	the pH stability of the ND-OH nanophase	1368:1406	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	56	theme	hydrogel	1519:1526	arg1	processing					1424:1433	filament-based processing	1409:1433	filament-based processing	1409:1433	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	56	theme	hydrogel	1519:1526	arg1	deposition					1451:1460	layer-based deposition	1439:1460	layer-based deposition at microscale attained	1439:1483	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	2	57	theme	/nanodiamond	370:381	arg1	hydrogels					337:345	3D printing nanocomposite hydrogels	311:345	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND)	311:386	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	58	from	pH	1152:1153	arg1	HA/ND-OH					1129:1136	HA/ND-OH	1129:1136	HA/ND-OH (0.04 wt%) at pH 7	1129:1155	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	58	from	pH	1152:1153	arg1	%					1146:1146	0.04 wt%	1139:1146	0.04 wt%	1139:1146	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	5	59	theme	critical	1293:1300	arg1	role					1302:1305	a critical role	1291:1305	a critical role	1291:1305	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	60	dep	%	828:828	arg1	616.72 kPa					831:840	616.72 kPa	831:840	0.04%: 616.72 kPa	824:840	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	6	61	theme	controlled	1583:1592	arg1	printing					1597:1604	controlled 3D printing	1583:1604	controlled 3D printing	1583:1604	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	4	62	theme	HA/ND-OH	1044:1051	arg1	stress					1034:1039	the compressive stress	1018:1039	the compressive stress of HA/ND-OH (0.04 wt%) at pH 8	1018:1070	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	5	63	theme	enforced	1485:1492	arg1	properties					1505:1514	enforced mechanical properties	1485:1514	enforced mechanical properties	1485:1514	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	1	64	theme	desired	257:263	arg1	properties					276:285	their desired mechanical properties	251:285	their desired mechanical properties	251:285	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	6	65	theme	nanophase	1569:1577	arg1	tuning					1542:1547	Fine surface tuning	1529:1547	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing	1529:1604	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	3	66	theme	functionalized	611:624	arg1	ND					626:627	surface functionalized ND	603:627	surface functionalized ND	603:627	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	6	67	theme	inorganic	1556:1564	arg1	nanophase					1569:1577	the inorganic ND nanophase	1552:1577	the inorganic ND nanophase	1552:1577	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	3	68	theme	compressive	654:664	arg1	stress					666:671	the enforced compressive stress	641:671	the enforced compressive stress	641:671	These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner.					
32522274	6	69	theme	Fine	1529:1532	arg1	tuning					1542:1547	Fine surface tuning	1529:1547	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing	1529:1604	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	1	70	theme	manufacturing	161:173	arg1	process					175:181	manufacturing process	161:181	manufacturing process	161:181	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	4	71	theme	0.02 wt	888:894	arg1	%					895:895	0.02 wt%	888:895	0.02 wt%	888:895	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	0	72	theme	acid	85:88	arg1	hydrogels					104:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	2	73	theme	3D	311:312	arg1	hydrogels					337:345	3D printing nanocomposite hydrogels	311:345	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND)	311:386	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	74	dep	%	794:794	arg1	236.18 kPa					797:806	236.18 kPa	797:806	0.02%: 236.18 kPa	790:806	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	4	75	theme	HA	711:712	arg1	hydrogels					728:736	HA nanocomposite hydrogels	711:736	HA nanocomposite hydrogels with ND-OH at pH 8	711:755	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	0	76	theme	hydrogels	104:112	arg1	properties					49:58	the mechanical properties	34:58	the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels	34:112	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	2	77	theme	breaking	504:511	arg1	point					513:517	gel breaking point	500:517	gel breaking point	500:517	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	1	78	from	attention	207:215	arg1	applications					231:242	biomedical applications	220:242	biomedical applications	220:242	Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality.					
32522274	2	79	theme	only	537:540	arg1	nanocomposites					542:555	HA only nanocomposites	534:555	HA only nanocomposites	534:555	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	80	theme	1.40-fold	779:787	arg1	increase					767:774	an increase	764:774	an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress	764:864	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	2	81	dep	resulted	459:466	arg1	compared					520:527	compared	520:527	compared with HA only nanocomposites	520:555	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	6	82	theme	printing	1597:1604	arg1	tuning					1542:1547	Fine surface tuning	1529:1547	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing	1529:1604	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	5	83	theme	properties	1343:1352	arg1	dependency					1314:1323	the dependency	1310:1323	the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel	1310:1526	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	2	84	theme	ND	418:419	arg1	addition					406:413	the addition	402:413	the addition of ND with the low weight ratio of 0.02 wt%	402:457	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	85	dep	pH.	1004:1006	arg1	enhanced					1089:1096	enhanced	1089:1096	enhanced	1089:1096	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	2	86	theme	%	457:457	arg1	ratio					441:445	the low weight ratio	426:445	the low weight ratio of 0.02 wt%	426:457	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	6	87	theme	nanocomposite	1683:1695	arg1	hydrogels					1697:1705	the nanocomposite hydrogels	1679:1705	the nanocomposite hydrogels reported herein	1679:1721	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	5	88	theme	ND-OH	1392:1396	arg1	nanophase					1398:1406	the ND-OH nanophase	1388:1406	the ND-OH nanophase	1388:1406	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	89	theme	tunable	1190:1196	arg1	environment					1208:1218	the tunable buffering environment	1186:1218	the tunable buffering environment	1186:1218	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	4	90	theme	1.37-fold	813:821	arg1	increase					767:774	an increase	764:774	an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress	764:864	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
32522274	5	91	theme	HA	1260:1261	arg1	chains					1250:1255	the long chains	1241:1255	the long chains of HA at the molecular level	1241:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	2	92	theme	weight	434:439	arg1	ratio					441:445	the low weight ratio	426:445	the low weight ratio of 0.02 wt%	426:457	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	0	93	theme	3D-printed	63:72	arg1	hydrogels					104:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	3D-printed hyaluronic acid nanocomposite hydrogels	63:112	pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels.					
32522274	2	94	theme	compressive	478:488	arg1	force					490:494	higher compressive force	471:494	higher compressive force	471:494	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	6	95	theme	mechanical	1654:1663	arg1	properties					1665:1674	the pH-dependent mechanical properties	1637:1674	the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein	1637:1721	Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.					
32522274	5	96	theme	pH	1372:1373	arg1	stability					1375:1383	the pH stability	1368:1383	the pH stability of the ND-OH nanophase	1368:1406	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	97	from	attained	1476:1483	arg1	stability					1375:1383	the pH stability	1368:1383	the pH stability of the ND-OH nanophase	1368:1406	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	97	from	attained	1476:1483	arg1	processing					1424:1433	filament-based processing	1409:1433	filament-based processing	1409:1433	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	97	from	attained	1476:1483	arg1	deposition					1451:1460	layer-based deposition	1439:1460	layer-based deposition at microscale attained	1439:1483	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	5	98	from	level	1280:1284	arg1	chains					1250:1255	the long chains	1241:1255	the long chains of HA at the molecular level	1241:1284	These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel.					
32522274	2	99	with	addition	406:413	arg1	ratio					441:445	the low weight ratio	426:445	the low weight ratio of 0.02 wt%	426:457	3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt% resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites.					
32522274	4	100	from	pH	1067:1068	arg1	stress					1034:1039	the compressive stress	1018:1039	the compressive stress of HA/ND-OH (0.04 wt%) at pH 8	1018:1070	HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40-fold (0.02%: 236.18 kPa) and 1.37-fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt% and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt%) at pH 8 was mechanically enhanced 1.29-fold, compared to that of HA/ND-OH (0.04 wt%) at pH 7.					
34058207	7	0	theme	curcumin	1390:1397	arg1	concentration					1399:1411	curcumin concentration	1390:1411	curcumin concentration of 0.20%	1390:1420	CUR-ME-G with curcumin concentration of 0.20% exhibited the best biological activity.					
34058207	2	1	with	CUR-ME1G3	708:716	arg1	GM					727:728	75% GM	723:728	75% GM	723:728	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	1	2	theme	starch	252:257	arg1	solution					259:266	the alginate-porous starch solution	232:266	the alginate-porous starch solution	232:266	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	3	3	theme	scanning	893:900	arg1	microscopy					911:920	scanning electron microscopy	893:920	scanning electron microscopy	893:920	Moreover, a denser structure that entrapped all microemulsion droplets was formed with increasing the proportion of microemulsion according to the observation of scanning electron microscopy.					
34058207	8	4	theme	delivery	1527:1534	arg1	systems					1536:1542	the smart drug delivery systems	1512:1542	the smart drug delivery systems	1512:1542	CUR-ME-G might provide a potential application in the smart drug delivery systems.					
34058207	6	5	theme	release	1280:1286	arg1	rate					1288:1291	The release rate	1276:1291	The release rate	1276:1291	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	3	6	theme	electron	902:909	arg1	microscopy					911:920	scanning electron microscopy	893:920	scanning electron microscopy	893:920	Moreover, a denser structure that entrapped all microemulsion droplets was formed with increasing the proportion of microemulsion according to the observation of scanning electron microscopy.					
34058207	6	7	theme	release	1360:1366	arg1	medium					1368:1373	the release medium	1356:1373	the release medium	1356:1373	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	8	8	theme	drug	1522:1525	arg1	systems					1536:1542	the smart drug delivery systems	1512:1542	the smart drug delivery systems	1512:1542	CUR-ME-G might provide a potential application in the smart drug delivery systems.					
34058207	1	9	theme	curcumin-loaded	321:335	arg1	microemulsion					337:349	curcumin-loaded microemulsion	321:349	curcumin-loaded microemulsion (CUR-ME)	321:358	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	9	theme	curcumin-loaded	321:335	arg1	CUR-ME					352:357	CUR-ME	352:357	CUR-ME	352:357	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	6	10	theme	medium	1368:1373	arg1	components					1309:1318	the components	1305:1318	the components of the CUR-ME-G	1305:1334	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	6	10	theme	medium	1368:1373	arg1	value					1347:1351	the pH value	1340:1351	the pH value of the release medium	1340:1373	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	1	11	theme	slow-gelling	405:416	arg1	agents					418:423	slow-gelling agents	405:423	slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G)	405:510	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	0	12	dep	in	113:114	arg1	vitro					116:120	vitro	116:120	vitro	116:120	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	2	13	theme	drug	661:664	arg1	capacity					674:681	the maximum drug loading capacity	649:681	the maximum drug loading capacity (14.9 mg/g)	649:693	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	13	theme	drug	661:664	arg1	mg/g					689:692	14.9 mg/g	684:692	14.9 mg/g	684:692	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	8	14	from	application	1497:1507	arg1	systems					1536:1542	the smart drug delivery systems	1512:1542	the smart drug delivery systems	1512:1542	CUR-ME-G might provide a potential application in the smart drug delivery systems.					
34058207	5	15	theme	in	1147:1148	arg1	release					1156:1162	The in vitro release	1143:1162	The in vitro release of the CUR-ME-G	1143:1178	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	7	16	with	CUR-ME-G	1376:1383	arg1	concentration					1399:1411	curcumin concentration	1390:1411	curcumin concentration of 0.20%	1390:1420	CUR-ME-G with curcumin concentration of 0.20% exhibited the best biological activity.					
34058207	2	17	theme	maximum	653:659	arg1	capacity					674:681	the maximum drug loading capacity	649:681	the maximum drug loading capacity (14.9 mg/g)	649:693	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	17	theme	maximum	653:659	arg1	mg/g					689:692	14.9 mg/g	684:692	14.9 mg/g	684:692	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	4	18	dep	Fourier	950:956	arg1	transform					958:966	transform	958:966	transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate	958:1140	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	0	19	theme	in	113:114	arg1	kinetics					130:137	in vitro release kinetics	113:137	in vitro release kinetics	113:137	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	2	20	located	found	699:703	arg1	CUR-ME1G3					708:716	CUR-ME1G3	708:716	CUR-ME1G3 with 75% GM	708:728	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	20	located	found	699:703	arg2	capacity					674:681	the maximum drug loading capacity	649:681	the maximum drug loading capacity (14.9 mg/g)	649:693	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	20	located	found	699:703	arg2	mg/g					689:692	14.9 mg/g	684:692	14.9 mg/g	684:692	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	20	located	found	699:703	arg2	efficiency					608:617	the drug loading efficiency	591:617	the drug loading efficiency increased from 24% to 98%	591:643	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	21	theme	loading	600:606	arg1	efficiency					608:617	the drug loading efficiency	591:617	the drug loading efficiency increased from 24% to 98%	591:643	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	5	22	theme	first-order	1249:1259	arg1	model					1269:1273	the first-order kinetic model	1245:1273	the first-order kinetic model	1245:1273	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	1	23	theme	controlled	179:188	arg1	release					190:196	the controlled release	175:196	the controlled release	175:196	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	0	24	theme	pH-sensitive	2:13	arg1	curcumin					15:22	A pH-sensitive curcumin	0:22	A pH-sensitive curcumin	0:22	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	2	25	theme	drug	595:598	arg1	efficiency					608:617	the drug loading efficiency	591:617	the drug loading efficiency increased from 24% to 98%	591:643	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	5	26	theme	kinetic	1261:1267	arg1	model					1269:1273	the first-order kinetic model	1245:1273	the first-order kinetic model	1245:1273	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	6	27	theme	pH	1344:1345	arg1	value					1347:1351	the pH value	1340:1351	the pH value of the release medium	1340:1373	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	3	28	theme	microscopy	911:920	arg1	observation					878:888	the observation	874:888	the observation of scanning electron microscopy	874:920	Moreover, a denser structure that entrapped all microemulsion droplets was formed with increasing the proportion of microemulsion according to the observation of scanning electron microscopy.					
34058207	5	29	dep	in	1147:1148	arg1	vitro					1150:1154	vitro	1150:1154	vitro	1150:1154	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	8	30	theme	smart	1516:1520	arg1	systems					1536:1542	the smart drug delivery systems	1512:1542	the smart drug delivery systems	1512:1542	CUR-ME-G might provide a potential application in the smart drug delivery systems.					
34058207	1	31	theme	gel	276:278	arg1	matrix					280:285	the gel matrix	272:285	the gel matrix (GM)	272:290	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	31	theme	gel	276:278	arg1	GM					288:289	GM	288:289	GM	288:289	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	4	32	dep	transform	958:966	arg1	infrared					968:975	infrared	968:975	transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate	958:1140	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	4	33	theme	Raman	994:998	arg1	spectroscopy					1000:1011	Raman spectroscopy	994:1011	Raman spectroscopy	994:1011	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	0	34	theme	release	122:128	arg1	kinetics					130:137	in vitro release kinetics	113:137	in vitro release kinetics	113:137	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	7	35	theme	best	1436:1439	arg1	activity					1452:1459	the best biological activity	1432:1459	the best biological activity	1432:1459	CUR-ME-G with curcumin concentration of 0.20% exhibited the best biological activity.					
34058207	6	36	theme	CUR-ME-G	1327:1334	arg1	components					1309:1318	the components	1305:1318	the components of the CUR-ME-G	1305:1334	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	6	36	theme	CUR-ME-G	1327:1334	arg1	value					1347:1351	the pH value	1340:1351	the pH value of the release medium	1340:1373	The release rate depended on the components of the CUR-ME-G and the pH value of the release medium.					
34058207	2	37	dep	%	636:636	arg1	to					638:639	to	638:639	to	638:639	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	0	38	theme	biological	143:152	arg1	activity					154:161	biological activity	143:161	biological activity	143:161	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	5	39	with	consistent	1229:1238	arg1	model					1269:1273	the first-order kinetic model	1245:1273	the first-order kinetic model	1245:1273	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	2	40	theme	loading	666:672	arg1	capacity					674:681	the maximum drug loading capacity	649:681	the maximum drug loading capacity (14.9 mg/g)	649:693	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	2	40	theme	loading	666:672	arg1	mg/g					689:692	14.9 mg/g	684:692	14.9 mg/g	684:692	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	0	41	theme	microemulsion-filled	31:50	arg1	alginate					52:59	microemulsion-filled alginate and porous starch composite gels	31:92	alginate	52:59	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	3	42	theme	microemulsion	779:791	arg1	droplets					793:800	all microemulsion droplets	775:800	all microemulsion droplets	775:800	Moreover, a denser structure that entrapped all microemulsion droplets was formed with increasing the proportion of microemulsion according to the observation of scanning electron microscopy.					
34058207	2	43	theme	24	634:635	arg1	%					636:636	%	636:636	%	636:636	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	1	44	theme	loaded	219:224	arg1	drug					226:229	the loaded drug	215:229	the loaded drug	215:229	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	0	45	theme	porous	65:70	arg1	gels					89:92	microemulsion-filled alginate and porous starch composite gels	31:92	gels	89:92	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	4	46	theme	new	1021:1023	arg1	peaks					1025:1029	no new peaks	1018:1029	no new peaks	1018:1029	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	2	47	theme	GM	547:548	arg1	proportion					533:542	the proportion	529:542	the proportion of GM	529:548	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	1	48	theme	certain	365:371	arg1	proportion					373:382	a certain proportion	363:382	a certain proportion	363:382	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	4	49	theme	hydrogen	1063:1070	arg1	interactions					1080:1091	the hydrogen bonding interactions	1059:1091	the hydrogen bonding interactions	1059:1091	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	5	50	theme	diffusion-controlled	1189:1208	arg1	mechanism					1210:1218	diffusion-controlled mechanism	1189:1218	diffusion-controlled mechanism that was consistent with the first-order kinetic model	1189:1273	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	1	51	theme	prepared	458:465	arg1	gel					497:499	prepared curcumin-loaded microemulsion gel	458:499	prepared curcumin-loaded microemulsion gel (CUR-ME-G)	458:510	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	51	theme	prepared	458:465	arg1	CUR-ME-G					502:509	CUR-ME-G	502:509	CUR-ME-G	502:509	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	7	52	theme	biological	1441:1450	arg1	activity					1452:1459	the best biological activity	1432:1459	the best biological activity	1432:1459	CUR-ME-G with curcumin concentration of 0.20% exhibited the best biological activity.					
34058207	8	53	theme	potential	1487:1495	arg1	application					1497:1507	a potential application	1485:1507	a potential application in the smart drug delivery systems	1485:1542	CUR-ME-G might provide a potential application in the smart drug delivery systems.					
34058207	7	54	theme	%	1420:1420	arg1	concentration					1399:1411	curcumin concentration	1390:1411	curcumin concentration of 0.20%	1390:1420	CUR-ME-G with curcumin concentration of 0.20% exhibited the best biological activity.					
34058207	1	55	theme	curcumin-loaded	467:481	arg1	gel					497:499	prepared curcumin-loaded microemulsion gel	458:499	prepared curcumin-loaded microemulsion gel (CUR-ME-G)	458:510	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	55	theme	curcumin-loaded	467:481	arg1	CUR-ME-G					502:509	CUR-ME-G	502:509	CUR-ME-G	502:509	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	56	dep	agents	418:423	arg1	CaCO3					426:430	CaCO3	426:430	CaCO3	426:430	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	56	dep	agents	418:423	arg1	d-glucono-δ-lactone					434:452	d-glucono-δ-lactone	434:452	d-glucono-δ-lactone	434:452	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	57	theme	drug	226:229	arg1	release					190:196	the controlled release	175:196	the controlled release	175:196	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	57	theme	drug	226:229	arg1	stability					202:210	stability	202:210	stability of the loaded drug	202:229	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	4	58	theme	bonding	1072:1078	arg1	interactions					1080:1091	the hydrogen bonding interactions	1059:1091	the hydrogen bonding interactions	1059:1091	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	1	59	theme	microemulsion	483:495	arg1	gel					497:499	prepared curcumin-loaded microemulsion gel	458:499	prepared curcumin-loaded microemulsion gel (CUR-ME-G)	458:510	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	1	59	theme	microemulsion	483:495	arg1	CUR-ME-G					502:509	CUR-ME-G	502:509	CUR-ME-G	502:509	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
34058207	0	60	theme	composite	79:87	arg1	gels					89:92	microemulsion-filled alginate and porous starch composite gels	31:92	gels	89:92	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	2	61	theme	98	641:642	arg1	%					636:636	%	636:636	%	636:636	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	5	62	theme	CUR-ME-G	1171:1178	arg1	release					1156:1162	The in vitro release	1143:1162	The in vitro release of the CUR-ME-G	1143:1178	The in vitro release of the CUR-ME-G followed diffusion-controlled mechanism that was consistent with the first-order kinetic model.					
34058207	4	63	theme	sodium	1126:1131	arg1	alginate					1133:1140	sodium alginate	1126:1140	sodium alginate	1126:1140	This was also confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy that no new peaks appeared in CUR-ME-G, while the hydrogen bonding interactions might exist between curcumin and sodium alginate.					
34058207	0	64	theme	starch	72:77	arg1	gels					89:92	microemulsion-filled alginate and porous starch composite gels	31:92	gels	89:92	A pH-sensitive curcumin loaded microemulsion-filled alginate and porous starch composite gels: Characterization, in vitro release kinetics and biological activity.					
34058207	3	65	theme	denser	743:748	arg1	structure					750:758	a denser structure	741:758	a denser structure that entrapped all microemulsion droplets	741:800	Moreover, a denser structure that entrapped all microemulsion droplets was formed with increasing the proportion of microemulsion according to the observation of scanning electron microscopy.					
34058207	2	66	theme	%	725:725	arg1	GM					727:728	75% GM	723:728	75% GM	723:728	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	3	67	theme	microemulsion	847:859	arg1	proportion					833:842	the proportion	829:842	the proportion of microemulsion	829:859	Moreover, a denser structure that entrapped all microemulsion droplets was formed with increasing the proportion of microemulsion according to the observation of scanning electron microscopy.					
34058207	2	68	theme	75	723:724	arg1	%					725:725	%	725:725	%	725:725	With increasing the proportion of GM from 25% (CUR-ME3G1) to 83% (CUR-ME1G5), the drug loading efficiency increased from 24% to 98% and the maximum drug loading capacity (14.9 mg/g) was found in CUR-ME1G3 with 75% GM.					
34058207	1	69	theme	alginate-porous	236:250	arg1	solution					259:266	the alginate-porous starch solution	232:266	the alginate-porous starch solution	232:266	To improve the controlled release and stability of the loaded drug, the alginate-porous starch solution, as the gel matrix (GM), was prepared and added into curcumin-loaded microemulsion (CUR-ME) in a certain proportion, and then mixed with slow-gelling agents (CaCO3 + d-glucono-δ-lactone) to prepared curcumin-loaded microemulsion gel (CUR-ME-G).					
31972331	6	0	dep	positions	1153:1161	arg1	604					1170:1172	604	1170:1172	604	1170:1172	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	16	1	theme	novel	2270:2274	arg1	residue					2287:2293	a novel amino acid residue	2268:2293	a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking	2268:2400	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	4	2	theme	expression	790:799	arg1	vector					801:806	the pcDNA6/V5-His B expression vector	770:806	the pcDNA6/V5-His B expression vector	770:806	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	6	3	theme	Sequencing	990:999	arg1	analyses					1001:1008	Sequencing analyses	990:1008	Sequencing analyses	990:1008	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	7	4	theme	[p.Q676R-p	1329:1338	arg1	combination					1300:1310	a combination	1298:1310	a combination of two mutations [p.Q676R-p	1298:1338	Expression of cDNAs encoding a combination of two mutations [p.Q676R-p.					
31972331	7	4	theme	[p.Q676R-p	1329:1338	arg1	Expression					1269:1278	Expression	1269:1278	Expression of cDNAs	1269:1287	Expression of cDNAs encoding a combination of two mutations [p.Q676R-p.					
31972331	6	5	theme	cloning	1091:1097	arg1	[p.L571P					1110:1117	cloning: c.1712T>C [p.L571P]	1091:1118	cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1091:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	14	6	theme	endoplasmic	2037:2047	arg1	reticulum					2049:2057	defective endoplasmic reticulum	2027:2057	defective endoplasmic reticulum exit of the mutant TG	2027:2079	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	11	7	theme	mutant	1509:1514	arg1	p.L571P					1516:1522	the single point mutant p.L571P	1492:1522	the single point mutant p.L571P	1492:1522	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	15	8	theme	linker	2122:2127	arg1	domain					2129:2134	the 3D linker domain	2115:2134	the 3D linker domain	2115:2134	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	11	9	theme	single	1496:1501	arg1	p.L571P					1516:1522	the single point mutant p.L571P	1492:1522	the single point mutant p.L571P	1492:1522	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	6	10	theme	acid	1148:1151	arg1	positions					1153:1161	amino acid positions 360 to 604	1142:1172	amino acid positions 360 to 604	1142:1172	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	10	theme	acid	1148:1151	arg1	domain					1134:1139	the linker domain	1123:1139	the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1123:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	15	11	theme	significant	2187:2197	arg1	change					2222:2227	a significant protein conformational change	2185:2227	a significant protein conformational change	2185:2227	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	16	12	theme	linker	2306:2311	arg1	TG					2323:2324	TG	2323:2324	TG	2323:2324	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	16	12	theme	linker	2306:2311	arg1	domain					2313:2318	the linker domain	2302:2318	the linker domain of TG associated with its conformational maturation and intracellular trafficking	2302:2400	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	4	13	theme	pcDNA6/V5-His	774:786	arg1	vector					801:806	the pcDNA6/V5-His B expression vector	770:806	the pcDNA6/V5-His B expression vector	770:806	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	2	14	dep	Rattus	246:251	arg1	norvegicus					253:262	norvegicus	253:262	norvegicus	253:262	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	1	15	theme	follicular	171:180	arg1	lumen					182:186	the thyroid follicular lumen	159:186	the thyroid follicular lumen	159:186	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	11	16	theme	HEK293T	1627:1633	arg1	cells					1635:1639	HEK293T cells	1627:1639	HEK293T cells	1627:1639	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	6	17	theme	type	1202:1205	arg1	repeat					1211:1216	TG type 1-6 repeat	1199:1216	TG type 1-6 repeat	1199:1216	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	15	18	theme	conformational	2207:2220	arg1	change					2222:2227	a significant protein conformational change	2185:2227	a significant protein conformational change	2185:2227	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	3	19	theme	adverse	490:496	arg1	effect					498:503	any adverse effect	486:503	any adverse effect on the TG protein	486:521	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	5	20	theme	culture	969:975	arg1	supernatant					977:987	the culture supernatant	965:987	the culture supernatant	965:987	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	12	21	theme	Golgi	1793:1797	arg1	apparatus					1799:1807	Golgi apparatus	1793:1807	Golgi apparatus	1793:1807	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	14	22	from	formation	1962:1970	arg1	apparatus					1985:1993	the Golgi apparatus	1975:1993	the Golgi apparatus	1975:1993	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	3	23	theme	TG	512:513	arg1	protein					515:521	the TG protein	508:521	the TG protein	508:521	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	2	24	theme	Rattus	246:251	arg1	rTG					268:270	rTG	268:270	rTG	268:270	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	2	24	theme	Rattus	246:251	arg1	TG					264:265	Rattus norvegicus TG	246:265	Rattus norvegicus TG (rTG)	246:271	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	4	25	theme	further	647:653	arg1	understanding					655:667	a further understanding	645:667	a further understanding of the protein domains regulating its intracellular fate	645:724	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	11	26	theme	intracellular	1587:1599	arg1	retention					1601:1609	intracellular retention	1587:1609	intracellular retention of mutant TG in HEK293T cells	1587:1639	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	13	27	theme	glycosylation	1925:1937	arg1	stage					1916:1920	the first stage	1906:1920	the first stage of glycosylation	1906:1937	This data suggest that the p.L571P mutant contains the mannose-type N-glycan, that was added at the first stage of glycosylation.					
31972331	7	28	theme	cDNAs	1283:1287	arg1	combination					1300:1310	a combination	1298:1310	a combination of two mutations [p.Q676R-p	1298:1338	Expression of cDNAs encoding a combination of two mutations [p.Q676R-p.					
31972331	7	28	theme	cDNAs	1283:1287	arg1	Expression					1269:1278	Expression	1269:1278	Expression of cDNAs	1269:1287	Expression of cDNAs encoding a combination of two mutations [p.Q676R-p.					
31972331	3	29	dep	impose	479:484	arg1	whereas					524:530	whereas	524:530	whereas	524:530	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	1	30	theme	large	104:108	arg1	Thyroglobulin					82:94	Thyroglobulin	82:94	Thyroglobulin (TG)	82:99	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	1	30	theme	large	104:108	arg1	protein					123:129	a large glycosylated protein	102:129	a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen	102:186	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	3	31	theme	other	532:536	arg1	substitutions					549:561	other nucleotide substitutions	532:561	other nucleotide substitutions	532:561	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	6	32	dep	domain	1134:1139	arg1	[p.Q676R					1186:1193	c.2027A>G [p.Q676R	1176:1193	c.2027A>G [p.Q676R	1176:1193	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	11	33	theme	TG	1621:1622	arg1	retention					1601:1609	intracellular retention	1587:1609	intracellular retention of mutant TG in HEK293T cells	1587:1639	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	12	34	theme	will-type	1740:1748	arg1	TG					1750:1751	the will-type TG	1736:1751	the will-type TG	1736:1751	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	0	35	from	p.L571P	0:6	arg1	domain					22:27	the linker domain	11:27	the linker domain of rat thyroglobulin	11:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	0	35	from	p.L571P	0:6	arg1	thyroglobulin					36:48	rat thyroglobulin	32:48	rat thyroglobulin	32:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	14	36	theme	N-glycan	1953:1960	arg1	formation					1962:1970	Complex-type N-glycan formation	1940:1970	Complex-type N-glycan formation in the Golgi apparatus	1940:1993	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	6	37	from	[p.L571P	1110:1117	arg1	positions					1153:1161	amino acid positions 360 to 604	1142:1172	amino acid positions 360 to 604	1142:1172	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	37	from	[p.L571P	1110:1117	arg1	domain					1134:1139	the linker domain	1123:1139	the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1123:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	38	theme	c.2720A>G	1222:1230	arg1	[p.Q907R					1232:1239	c.2720A>G [p.Q907R]	1222:1240	c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1222:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	5	39	theme	encoded	884:890	arg1	molecule					920:927	a wild-type molecule	908:927	a wild-type molecule	908:927	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	5	39	theme	encoded	884:890	arg1	protein					892:898	the encoded protein	880:898	the encoded protein	880:898	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	1	40	theme	glycosylated	110:121	arg1	Thyroglobulin					82:94	Thyroglobulin	82:94	Thyroglobulin (TG)	82:99	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	1	40	theme	glycosylated	110:121	arg1	protein					123:129	a large glycosylated protein	102:129	a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen	102:186	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	4	41	theme	intracellular	707:719	arg1	fate					721:724	its intracellular fate	703:724	its intracellular fate	703:724	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	3	42	from	effect	498:503	arg1	protein					515:521	the TG protein	508:521	the TG protein	508:521	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	6	43	from	[p.Q907R	1232:1239	arg1	repeat					1261:1266	the TG type 1-7 repeat	1245:1266	the TG type 1-7 repeat	1245:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	16	44	theme	intracellular	2376:2388	arg1	trafficking					2390:2400	intracellular trafficking	2376:2400	intracellular trafficking	2376:2400	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	2	45	theme	8461-kb	382:388	arg1	mRNA					390:393	a 8461-kb mRNA	380:393	a 8461-kb mRNA	380:393	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	0	46	theme	intracellular	57:69	arg1	retention					71:79	intracellular retention	57:79	intracellular retention	57:79	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	2	47	theme	located	326:332	arg1	gene					307:310	a large single copy gene	287:310	a large single copy gene	287:310	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	2	47	theme	located	326:332	arg1	long					320:323	long	320:323	long	320:323	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	15	48	theme	domain	2129:2134	arg1	analysis					2103:2110	predictive analysis	2092:2110	predictive analysis of the 3D linker domain	2092:2134	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	4	49	theme	protein	676:682	arg1	domains					684:690	the protein domains	672:690	the protein domains regulating its intracellular fate	672:724	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	15	50	theme	predictive	2092:2101	arg1	analysis					2103:2110	predictive analysis	2092:2110	predictive analysis of the 3D linker domain	2092:2134	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	16	51	theme	TG	2323:2324	arg1	TG					2323:2324	TG	2323:2324	TG	2323:2324	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	16	51	theme	TG	2323:2324	arg1	domain					2313:2318	the linker domain	2302:2318	the linker domain of TG associated with its conformational maturation and intracellular trafficking	2302:2400	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	3	52	theme	TG	578:579	arg1	stability					581:589	its TG stability	574:589	its TG stability	574:589	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	4	53	theme	full-length	739:749	arg1	cDNA					751:754	a full-length cDNA	737:754	a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector	737:806	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	14	54	theme	TG	2078:2079	arg1	exit					2059:2062	defective endoplasmic reticulum exit	2027:2062	defective endoplasmic reticulum exit of the mutant TG	2027:2079	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	2	55	theme	copy	302:305	arg1	gene					307:310	a large single copy gene	287:310	a large single copy gene	287:310	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	2	55	theme	copy	302:305	arg1	long					320:323	long	320:323	long	320:323	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	6	56	theme	type	1252:1255	arg1	repeat					1261:1266	the TG type 1-7 repeat	1245:1266	the TG type 1-7 repeat	1245:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	16	57	theme	conformational	2346:2359	arg1	maturation					2361:2370	conformational maturation	2346:2370	conformational maturation	2346:2370	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	3	58	theme	intracellular	601:613	arg1	trafficking					615:625	TG intracellular trafficking	598:625	TG intracellular trafficking	598:625	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	13	59	theme	mannose-type	1865:1876	arg1	N-glycan					1878:1885	the mannose-type N-glycan	1861:1885	the mannose-type N-glycan	1861:1885	This data suggest that the p.L571P mutant contains the mannose-type N-glycan, that was added at the first stage of glycosylation.					
31972331	3	60	theme	TG	409:410	arg1	gene					412:415	the TG gene	405:415	the TG gene	405:415	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	1	61	theme	essential	198:206	arg1	role					208:211	an essential role	195:211	an essential role	195:211	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	12	62	from	modifications	1776:1788	arg1	apparatus					1799:1807	Golgi apparatus	1793:1807	Golgi apparatus	1793:1807	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	14	63	theme	mutant	2071:2076	arg1	TG					2078:2079	the mutant TG	2067:2079	the mutant TG	2067:2079	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	3	64	theme	many	448:451	arg1	mutations					462:470	many missense mutations	448:470	many missense mutations	448:470	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	6	65	theme	1-7	1257:1259	arg1	repeat					1261:1266	the TG type 1-7 repeat	1245:1266	the TG type 1-7 repeat	1245:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	16	66	theme	amino	2276:2280	arg1	residue					2287:2293	a novel amino acid residue	2268:2293	a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking	2268:2400	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	12	67	theme	Endo	1642:1645	arg1	analysis					1649:1656	Endo H analysis	1642:1656	Endo H analysis	1642:1656	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	13	68	theme	p.L571P	1837:1843	arg1	mutant					1845:1850	the p.L571P mutant	1833:1850	the p.L571P mutant	1833:1850	This data suggest that the p.L571P mutant contains the mannose-type N-glycan, that was added at the first stage of glycosylation.					
31972331	1	69	theme	hormone	224:230	arg1	biosynthesis					232:243	thyroid hormone biosynthesis	216:243	thyroid hormone biosynthesis	216:243	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	7	70	theme	mutations	1319:1327	arg1	[p.Q676R-p					1329:1338	two mutations [p.Q676R-p	1315:1338	two mutations [p.Q676R-p	1315:1338	Expression of cDNAs encoding a combination of two mutations [p.Q676R-p.					
31972331	4	71	theme	B	788:788	arg1	vector					801:806	the pcDNA6/V5-His B expression vector	770:806	the pcDNA6/V5-His B expression vector	770:806	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	14	72	theme	reticulum	2049:2057	arg1	exit					2059:2062	defective endoplasmic reticulum exit	2027:2062	defective endoplasmic reticulum exit of the mutant TG	2027:2079	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	15	73	theme	3D	2119:2120	arg1	domain					2129:2134	the 3D linker domain	2115:2134	the 3D linker domain	2115:2134	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	14	74	theme	defective	2027:2035	arg1	reticulum					2049:2057	defective endoplasmic reticulum	2027:2057	defective endoplasmic reticulum exit of the mutant TG	2027:2079	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	6	75	dep	604	1170:1172	arg1	to					1167:1168	to	1167:1168	to	1167:1168	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	11	76	theme	point	1503:1507	arg1	p.L571P					1516:1522	the single point mutant p.L571P	1492:1522	the single point mutant p.L571P	1492:1522	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	6	77	theme	amino	1142:1146	arg1	positions					1153:1161	amino acid positions 360 to 604	1142:1172	amino acid positions 360 to 604	1142:1172	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	77	theme	amino	1142:1146	arg1	domain					1134:1139	the linker domain	1123:1139	the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1123:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	1	78	theme	thyroid	163:169	arg1	lumen					182:186	the thyroid follicular lumen	159:186	the thyroid follicular lumen	159:186	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	0	79	theme	rat	32:34	arg1	thyroglobulin					36:48	rat thyroglobulin	32:48	rat thyroglobulin	32:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	10	80	theme	p.L571P	1421:1427	arg1	substitution					1429:1440	the p.L571P substitution	1417:1440	the p.L571P substitution	1417:1440	Q676R] indicated that any TG bearing the p.L571P substitution was trapped intracellularly.					
31972331	2	81	theme	single	295:300	arg1	gene					307:310	a large single copy gene	287:310	a large single copy gene	287:310	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	2	81	theme	single	295:300	arg1	long					320:323	long	320:323	long	320:323	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	6	82	theme	TG	1199:1200	arg1	repeat					1211:1216	TG type 1-6 repeat	1199:1216	TG type 1-6 repeat	1199:1216	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	12	83	theme	N-glycan	1767:1774	arg1	modifications					1776:1788	full N-glycan modifications	1762:1788	full N-glycan modifications in Golgi apparatus	1762:1807	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	6	84	theme	linker	1127:1132	arg1	positions					1153:1161	amino acid positions 360 to 604	1142:1172	amino acid positions 360 to 604	1142:1172	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	84	theme	linker	1127:1132	arg1	domain					1134:1139	the linker domain	1123:1139	the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1123:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	14	85	theme	Golgi	1979:1983	arg1	apparatus					1985:1993	the Golgi apparatus	1975:1993	the Golgi apparatus	1975:1993	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	15	86	theme	protein	2199:2205	arg1	change					2222:2227	a significant protein conformational change	2185:2227	a significant protein conformational change	2185:2227	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	3	87	theme	nucleotide	538:547	arg1	substitutions					549:561	other nucleotide substitutions	532:561	other nucleotide substitutions	532:561	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	5	88	theme	cDNA	846:849	arg1	expression					828:837	transient expression	818:837	transient expression of the cDNA in HEK293T cells	818:866	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	11	89	from	retention	1601:1609	arg1	cells					1635:1639	HEK293T cells	1627:1639	HEK293T cells	1627:1639	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	6	90	theme	[p.L571P	1110:1117	arg1	course					1081:1086	the course	1077:1086	the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1077:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	91	from	domain	1134:1139	arg1	course					1081:1086	the course	1077:1086	the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1077:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	91	from	domain	1134:1139	arg1	repeat					1211:1216	TG type 1-6 repeat	1199:1216	TG type 1-6 repeat	1199:1216	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	91	from	domain	1134:1139	arg1	[p.Q907R					1232:1239	c.2720A>G [p.Q907R]	1222:1240	c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1222:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	2	92	theme	large	289:293	arg1	gene					307:310	a large single copy gene	287:310	a large single copy gene	287:310	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	2	92	theme	large	289:293	arg1	long					320:323	long	320:323	long	320:323	Rattus norvegicus TG (rTG) is encoded by a large single copy gene, 186-kb long, located on chromosome 7 composed of 48 exons encoding a 8461-kb mRNA.					
31972331	6	93	theme	TG	1249:1250	arg1	repeat					1261:1266	the TG type 1-7 repeat	1245:1266	the TG type 1-7 repeat	1245:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	15	94	theme	p.L571P	2152:2158	arg1	mutation					2160:2167	the p.L571P mutation	2148:2167	the p.L571P mutation	2148:2167	Moreover, predictive analysis of the 3D linker domain showed that the p.L571P mutation would result in a significant protein conformational change.					
31972331	5	95	theme	transient	818:826	arg1	expression					828:837	transient expression	818:837	transient expression of the cDNA in HEK293T cells	818:866	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	13	96	theme	first	1910:1914	arg1	stage					1916:1920	the first stage	1906:1920	the first stage of glycosylation	1906:1937	This data suggest that the p.L571P mutant contains the mannose-type N-glycan, that was added at the first stage of glycosylation.					
31972331	12	97	theme	full	1762:1765	arg1	modifications					1776:1788	full N-glycan modifications	1762:1788	full N-glycan modifications in Golgi apparatus	1762:1807	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	5	98	from	expression	828:837	arg1	cells					862:866	HEK293T cells	854:866	HEK293T cells	854:866	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	14	99	theme	Complex-type	1940:1951	arg1	formation					1962:1970	Complex-type N-glycan formation	1940:1970	Complex-type N-glycan formation in the Golgi apparatus	1940:1993	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	4	100	theme	domains	684:690	arg1	understanding					655:667	a further understanding	645:667	a further understanding of the protein domains regulating its intracellular fate	645:724	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	6	101	from	course	1081:1086	arg1	positions					1153:1161	amino acid positions 360 to 604	1142:1172	amino acid positions 360 to 604	1142:1172	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	6	101	from	course	1081:1086	arg1	domain					1134:1139	the linker domain	1123:1139	the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat	1123:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	11	102	theme	mutant	1614:1619	arg1	TG					1621:1622	mutant TG	1614:1622	mutant TG	1614:1622	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	6	103	from	repeat	1211:1216	arg1	repeat					1261:1266	the TG type 1-7 repeat	1245:1266	the TG type 1-7 repeat	1245:1266	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	14	104	with	consistent	2011:2020	arg1	exit					2059:2062	defective endoplasmic reticulum exit	2027:2062	defective endoplasmic reticulum exit of the mutant TG	2027:2079	Complex-type N-glycan formation in the Golgi apparatus does not occur, consistent with defective endoplasmic reticulum exit of the mutant TG.					
31972331	4	105	from	rTG	761:763	arg1	cDNA					751:754	a full-length cDNA	737:754	a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector	737:806	In order to gain a further understanding of the protein domains regulating its intracellular fate, we cloned a full-length cDNA from rTG into the pcDNA6/V5-His B expression vector.					
31972331	5	106	theme	HEK293T	854:860	arg1	cells					862:866	HEK293T cells	854:866	HEK293T cells	854:866	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	1	107	gly	glycosylated	110:121	arg1	Thyroglobulin					82:94	Thyroglobulin	82:94	Thyroglobulin (TG)	82:99	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	1	107	gly	glycosylated	110:121	arg1	protein					123:129	a large glycosylated protein	102:129	a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen	102:186	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
31972331	0	108	theme	linker	15:20	arg1	domain					22:27	the linker domain	11:27	the linker domain of rat thyroglobulin	11:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	0	108	theme	linker	15:20	arg1	thyroglobulin					36:48	rat thyroglobulin	32:48	rat thyroglobulin	32:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	0	109	theme	thyroglobulin	36:48	arg1	domain					22:27	the linker domain	11:27	the linker domain of rat thyroglobulin	11:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	0	109	theme	thyroglobulin	36:48	arg1	thyroglobulin					36:48	rat thyroglobulin	32:48	rat thyroglobulin	32:48	p.L571P in the linker domain of rat thyroglobulin causes intracellular retention.					
31972331	12	110	theme	p.L571P	1674:1680	arg1	mutant					1682:1687	the p.L571P mutant	1670:1687	the p.L571P mutant	1670:1687	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	12	110	theme	p.L571P	1674:1680	arg1	sensitive					1703:1711	sensitive	1703:1711	sensitive	1703:1711	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	12	111	theme	H	1647:1647	arg1	analysis					1649:1656	Endo H analysis	1642:1656	Endo H analysis	1642:1656	Endo H analysis showed that the p.L571P mutant is completely sensitive to the enzyme, whereas the will-type TG acquires full N-glycan modifications in Golgi apparatus.					
31972331	3	112	theme	TG	598:599	arg1	trafficking					615:625	TG intracellular trafficking	598:625	TG intracellular trafficking	598:625	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	3	113	theme	sequence	426:433	arg1	variability					435:445	sequence variability	426:445	sequence variability	426:445	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	6	114	theme	random	1025:1030	arg1	mutations					1032:1040	three random mutations	1019:1040	three random mutations	1019:1040	Sequencing analyses revealed three random mutations, which accidentally emerged during the course of cloning: c.1712T>C [p.L571P] in the linker domain (amino acid positions 360 to 604), c.2027A>G [p.Q676R] in TG type 1-6 repeat and c.2720A>G [p.Q907R] in the TG type 1-7 repeat.					
31972331	11	115	theme	point	1548:1552	arg1	mutation					1554:1561	this point mutation	1543:1561	this point mutation	1543:1561	Indeed, we expressed the single point mutant p.L571P and confirmed that this point mutation was sufficient to cause intracellular retention of mutant TG in HEK293T cells.					
31972331	16	116	theme	acid	2282:2285	arg1	residue					2287:2293	a novel amino acid residue	2268:2293	a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking	2268:2400	In conclusion, our studies identified a novel amino acid residue within the linker domain of TG associated with its conformational maturation and intracellular trafficking.					
31972331	5	117	theme	wild-type	910:918	arg1	molecule					920:927	a wild-type molecule	908:927	a wild-type molecule	908:927	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	5	117	theme	wild-type	910:918	arg1	protein					892:898	the encoded protein	880:898	the encoded protein	880:898	However, transient expression of the cDNA in HEK293T cells showed that the encoded protein was not a wild-type molecule, as it was unable to be secreted in the culture supernatant.					
31972331	13	118	contain	contains	1852:1859	arg2	N-glycan					1878:1885	the mannose-type N-glycan	1861:1885	the mannose-type N-glycan	1861:1885	This data suggest that the p.L571P mutant contains the mannose-type N-glycan, that was added at the first stage of glycosylation.					
31972331	13	118	contain	contains	1852:1859	arg1	mutant					1845:1850	the p.L571P mutant	1833:1850	the p.L571P mutant	1833:1850	This data suggest that the p.L571P mutant contains the mannose-type N-glycan, that was added at the first stage of glycosylation.					
31972331	3	119	theme	missense	453:460	arg1	mutations					462:470	many missense mutations	448:470	many missense mutations	448:470	Although the TG gene displays sequence variability, many missense mutations do not impose any adverse effect on the TG protein, whereas other nucleotide substitutions may affect its TG stability and/or TG intracellular trafficking.					
31972331	1	120	theme	thyroid	216:222	arg1	biosynthesis					232:243	thyroid hormone biosynthesis	216:243	thyroid hormone biosynthesis	216:243	Thyroglobulin (TG), a large glycosylated protein secreted by thyrocytes into the thyroid follicular lumen, plays an essential role in thyroid hormone biosynthesis.					
32451641	0	0	theme	tuber	80:84	arg1	cultivation					86:96	tuber cultivation	80:96	tuber cultivation	80:96	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	2	1	theme	other	418:422	arg1	elements					430:437	other trace elements	418:437	other trace elements	418:437	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	7	2	theme	non-destructive	1014:1028	arg1	assessment					1030:1039	completely non-invasive and non-destructive assessment	986:1039	completely non-invasive and non-destructive assessment of nutrient content of potato tubers	986:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	4	3	theme	tubers	622:627	arg1	value					606:610	the nutritional value	590:610	the nutritional value of potato tubers	590:627	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	4	4	theme	potato	615:620	arg1	tubers					622:627	potato tubers	615:627	potato tubers	615:627	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	1	5	theme	staple	247:252	arg1	potatoes					216:223	potatoes	216:223	potatoes (Solanum tuberosum) a staple	216:252	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	7	6	theme	non-invasive	997:1008	arg1	assessment					1030:1039	completely non-invasive and non-destructive assessment	986:1039	completely non-invasive and non-destructive assessment of nutrient content of potato tubers	986:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	4	7	theme	spectroscopic	679:691	arg1	methods					693:699	various wet chemistry and spectroscopic methods	653:699	various wet chemistry and spectroscopic methods	653:699	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	4	8	theme	wet	661:663	arg1	methods					693:699	various wet chemistry and spectroscopic methods	653:699	various wet chemistry and spectroscopic methods	653:699	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	0	9	theme	cultivation	86:96	arg1	origin					70:75	the origin	66:75	the origin of tuber cultivation	66:96	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	0	10	theme	offset	114:119	arg1	spectroscopy					127:138	spatially offset Raman spectroscopy	104:138	spatially offset Raman spectroscopy	104:138	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	5	11	theme	several	749:755	arg1	assays					780:785	several different colorimetric assays	749:785	several different colorimetric assays	749:785	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	8	12	theme	potato	1139:1144	arg1	varieties					1146:1154	nine different potato varieties	1124:1154	nine different potato varieties	1124:1154	We also show that RS can be used to identify nine different potato varieties, as well as determine the origin of their cultivation.					
32451641	2	13	theme	%	347:347	arg1	carbohydrates					349:361	12% carbohydrates	345:361	12% carbohydrates	345:361	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	7	14	theme	nutrient	1044:1051	arg1	content					1053:1059	nutrient content	1044:1059	nutrient content of potato tubers	1044:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	1	15	theme	cultivation	176:186	arg1	content					153:159	High starch content	141:159	High starch content	141:159	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	1	15	theme	cultivation	176:186	arg1	simplicity					162:171	simplicity	162:171	simplicity of cultivation	162:186	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	1	15	theme	cultivation	176:186	arg1	productivity					198:209	high productivity	193:209	high productivity	193:209	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	4	16	theme	various	653:659	arg1	methods					693:699	various wet chemistry and spectroscopic methods	653:699	various wet chemistry and spectroscopic methods	653:699	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	4	17	theme	nutritional	594:604	arg1	value					606:610	the nutritional value	590:610	the nutritional value of potato tubers	590:627	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	0	18	theme	Non-invasive	0:11	arg1	identification					13:26	Non-invasive identification	0:26	Non-invasive identification of potato varieties	0:46	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	3	19	theme	location	499:506	arg1	type					480:483	the type	476:483	the type of potato and location	476:506	These proportions vary depending on the type of potato and location where they were cultivated.					
32451641	8	20	theme	different	1129:1137	arg1	varieties					1146:1154	nine different potato varieties	1124:1154	nine different potato varieties	1124:1154	We also show that RS can be used to identify nine different potato varieties, as well as determine the origin of their cultivation.					
32451641	0	21	theme	potato	31:36	arg1	varieties					38:46	potato varieties	31:46	potato varieties	31:46	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	9	22	theme	portable	1215:1222	arg1	nature					1224:1229	The portable nature	1211:1229	The portable nature of Raman-based identification of potato	1211:1269	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	7	23	theme	spectroscopy	964:975	arg1	use					951:953	the use	947:953	the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers	947:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	4	24	theme	chemistry	665:673	arg1	methods					693:699	various wet chemistry and spectroscopic methods	653:699	various wet chemistry and spectroscopic methods	653:699	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	1	25	theme	high	193:196	arg1	productivity					198:209	high productivity	193:209	high productivity	193:209	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	2	26	theme	remaining	372:380	arg1	%					383:383	the remaining 4%	368:383	the remaining 4%	368:383	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	1	27	theme	people	269:274	arg1	diet					261:264	the diet	257:264	the diet of people around the world	257:291	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	0	28	theme	Raman	121:125	arg1	spectroscopy					127:138	spatially offset Raman spectroscopy	104:138	spatially offset Raman spectroscopy	104:138	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	3	29	theme	potato	488:493	arg1	type					480:483	the type	476:483	the type of potato and location	476:506	These proportions vary depending on the type of potato and location where they were cultivated.					
32451641	1	30	theme	starch	146:151	arg1	content					153:159	High starch content	141:159	High starch content	141:159	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	7	31	theme	content	1053:1059	arg1	assessment					1030:1039	completely non-invasive and non-destructive assessment	986:1039	completely non-invasive and non-destructive assessment of nutrient content of potato tubers	986:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	8	32	theme	cultivation	1198:1208	arg1	origin					1182:1187	the origin	1178:1187	the origin of their cultivation	1178:1208	We also show that RS can be used to identify nine different potato varieties, as well as determine the origin of their cultivation.					
32451641	5	33	theme	different	757:765	arg1	assays					780:785	several different colorimetric assays	749:785	several different colorimetric assays	749:785	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	4	34	theme	chemical	558:565	arg1	composition					567:577	the chemical composition	554:577	the chemical composition	554:577	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	0	35	theme	varieties	38:46	arg1	prediction					52:61	prediction	52:61	prediction of the origin of tuber cultivation	52:96	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	0	35	theme	varieties	38:46	arg1	identification					13:26	Non-invasive identification	0:26	Non-invasive identification of potato varieties	0:46	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	9	36	theme	Raman-based	1234:1244	arg1	identification					1246:1259	Raman-based identification	1234:1259	Raman-based identification of potato	1234:1269	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	7	37	theme	Raman	958:962	arg1	RS					978:979	RS	978:979	RS	978:979	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	7	37	theme	Raman	958:962	arg1	spectroscopy					964:975	Raman spectroscopy	958:975	Raman spectroscopy (RS)	958:980	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	7	38	theme	potato	1064:1069	arg1	tubers					1071:1076	potato tubers	1064:1076	potato tubers	1064:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	5	39	theme	colorimetric	767:778	arg1	assays					780:785	several different colorimetric assays	749:785	several different colorimetric assays	749:785	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	9	40	theme	potato	1333:1338	arg1	harvesting					1340:1349	potato harvesting	1333:1349	potato harvesting	1333:1349	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	8	41	used	used	1107:1110	arg2	RS					1097:1098	RS	1097:1098	RS	1097:1098	We also show that RS can be used to identify nine different potato varieties, as well as determine the origin of their cultivation.					
32451641	9	42	theme	quality	1367:1373	arg1	evaluation					1375:1384	quick quality evaluation	1361:1384	quick quality evaluation	1361:1384	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	5	43	theme	starch	819:824	arg1	content					826:832	the starch content	815:832	the starch content	815:832	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	5	44	theme	gravity	716:722	arg1	measurements					724:735	gravity measurements	716:735	gravity measurements	716:735	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	5	45	used	used	795:798	arg2	measurements					724:735	gravity measurements	716:735	gravity measurements	716:735	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	5	45	used	used	795:798	arg2	assays					780:785	several different colorimetric assays	749:785	several different colorimetric assays	749:785	For instance, gravity measurements, as well as several different colorimetric assays, can be used to investigate the starch content.					
32451641	9	46	theme	quick	1361:1365	arg1	evaluation					1375:1384	quick quality evaluation	1361:1384	quick quality evaluation	1361:1384	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	9	47	theme	potato	1264:1269	arg1	identification					1246:1259	Raman-based identification	1234:1259	Raman-based identification of potato	1234:1269	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	6	48	dep	indirect	865:872	arg1	destructive					881:891	destructive	881:891	destructive	881:891	However, these approaches are indirect, often destructive, and time- and labor-consuming.					
32451641	9	49	theme	such	1305:1308	arg1	analysis					1310:1317	such analysis	1305:1317	such analysis	1305:1317	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
32451641	1	50	dep	potatoes	216:223	arg1	tuberosum					234:242	Solanum tuberosum	226:242	Solanum tuberosum	226:242	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	1	51	theme	High	141:144	arg1	content					153:159	High starch content	141:159	High starch content	141:159	High starch content, simplicity of cultivation, and high productivity make potatoes (Solanum tuberosum) a staple in the diet of people around the world.					
32451641	0	52	theme	origin	70:75	arg1	prediction					52:61	prediction	52:61	prediction of the origin of tuber cultivation	52:96	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	0	52	theme	origin	70:75	arg1	identification					13:26	Non-invasive identification	0:26	Non-invasive identification of potato varieties	0:46	Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.					
32451641	10	53	dep	abstract	1397:1404	arg1	Graphical					1387:1395	Graphical	1387:1395	Graphical	1387:1395	Graphical abstract.					
32451641	2	54	theme	%	333:333	arg1	water					335:339	83% water	331:339	83% water	331:339	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	2	55	theme	12	345:346	arg1	%					347:347	%	347:347	%	347:347	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	4	56	theme	same	543:546	arg1	time					548:551	the same time	539:551	the same time	539:551	At the same time, the chemical composition determines the nutritional value of potato tubers and can be proved using various wet chemistry and spectroscopic methods.					
32451641	2	57	theme	trace	424:428	arg1	elements					430:437	other trace elements	418:437	other trace elements	418:437	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	7	58	theme	tubers	1071:1076	arg1	content					1053:1059	nutrient content	1044:1059	nutrient content of potato tubers	1044:1076	This study reports on the use of Raman spectroscopy (RS) for completely non-invasive and non-destructive assessment of nutrient content of potato tubers.					
32451641	2	59	theme	83	331:332	arg1	%					333:333	%	333:333	%	333:333	On average, potatoes are composed of 83% water and 12% carbohydrates, and the remaining 4% includes proteins, vitamins, and other trace elements.					
32451641	9	60	theme	identification	1246:1259	arg1	nature					1224:1229	The portable nature	1211:1229	The portable nature of Raman-based identification of potato	1211:1269	The portable nature of Raman-based identification of potato offers the possibility to perform such analysis directly upon potato harvesting to enable quick quality evaluation.					
31997346	1	0	theme	filter	321:326	arg1	cakes					328:332	filter cakes	321:332	filter cakes	321:332	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	4	1	from	RESULTS	709:715	arg1	dispersion					734:743	general, good dispersion	720:743	general, good dispersion of the lipid material	720:765	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	1	2	theme	aqueous	151:157	arg1	emulsions					159:167	aqueous emulsions	151:167	aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil	151:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	7	3	theme	lower	1225:1229	arg1	content					1238:1244	lower pectin content	1225:1244	lower pectin content	1225:1244	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	2	4	dep	proportions	522:532	arg1	g-1					554:556	0.1, 0.2 and 0.3 g g-1	535:556	g-1	554:556	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	1	5	theme	cakes	328:332	arg1	samples					310:316	two waste samples	300:316	two waste samples of filter cakes produced in the winterization process of sunflower oil	300:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	0	6	theme	sunflower	91:99	arg1	waxes					101:105	purified sunflower waxes	82:105	purified sunflower waxes	82:105	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	5	7	from	Increase	815:822	arg1	resistance					837:846	the water resistance	827:846	the water resistance (lower swelling index and water adsorption)	827:890	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	6	8	theme	wax	1076:1078	arg1	composition					1107:1117	their fatty acid composition	1090:1117	their fatty acid composition	1090:1117	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	8	theme	wax	1076:1078	arg1	addition					1080:1087	the wax addition	1072:1087	the wax addition	1072:1087	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	8	theme	wax	1076:1078	arg1	distribution					1134:1145	their good distribution	1123:1145	their good distribution	1123:1145	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	4	9	located	observed	771:778	arg2	RESULTS					709:715	RESULTS	709:715	RESULTS In general, good dispersion of the lipid material	709:765	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	4	9	located	observed	771:778	arg1	cross-sections					787:800	the cross-sections	783:800	the cross-sections of the film	783:812	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	8	10	dep	CONCLUSION	1338:1347	arg1	evidence					1363:1370	evidence	1363:1370	evidence	1363:1370	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	0	11	theme	purified	82:89	arg1	waxes					101:105	purified sunflower waxes	82:105	purified sunflower waxes	82:105	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	3	12	theme	water	639:643	arg1	permeability					651:662	water vapor permeability	639:662	water vapor permeability	639:662	Films were evaluated according to their structure, water resistance, water vapor permeability, mechanical properties and thermal behavior.					
31997346	2	13	theme	0.1	535:537	arg1	g-1					554:556	0.1, 0.2 and 0.3 g g-1	535:556	g-1	554:556	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	5	14	theme	water	874:878	arg1	adsorption					880:889	water adsorption	874:889	water adsorption	874:889	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	2	15	dep	added	507:511	arg1	HOSFW					495:499	HOSFW	495:499	HOSFW	495:499	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	2	15	dep	added	507:511	arg1	hybrid					449:454	the normal hybrid	438:454	the normal hybrid	438:454	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	2	15	dep	added	507:511	arg1	NSFW					457:460	NSFW	457:460	NSFW	457:460	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	3	16	theme	vapor	645:649	arg1	permeability					651:662	water vapor permeability	639:662	water vapor permeability	639:662	Films were evaluated according to their structure, water resistance, water vapor permeability, mechanical properties and thermal behavior.					
31997346	1	17	from	samples	277:283	arg1	pectin					196:201	low-methoxyl pectin	183:201	low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil	183:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	2	18	theme	sunflower	398:406	arg1	samples					414:420	The two sunflower waxes samples	390:420	The two sunflower waxes samples recovered	390:430	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	6	19	from	effect	1062:1067	arg1	film					1154:1157	the film	1150:1157	the film	1150:1157	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	20	theme	good	1129:1132	arg1	addition					1080:1087	the wax addition	1072:1087	the wax addition	1072:1087	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	20	theme	good	1129:1132	arg1	distribution					1134:1145	their good distribution	1123:1145	their good distribution	1123:1145	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	8	21	theme	promising	1374:1382	arg1	alternative					1384:1394	a promising alternative	1372:1394	a promising alternative	1372:1394	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	1	22	theme	winterization	350:362	arg1	process					364:370	the winterization process	346:370	the winterization process of sunflower oil	346:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	4	23	theme	lipid	752:756	arg1	material					758:765	the lipid material	748:765	the lipid material	748:765	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	8	24	theme	strategies	1429:1438	arg1	development					1403:1413	the development	1399:1413	the development of innovative strategies to valorize sunflower waxes derived from waste material	1399:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	7	25	theme	elongation	1308:1317	arg1	values					1330:1335	elongation percentage values	1308:1335	elongation percentage values	1308:1335	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	4	26	dep	general	720:726	arg1	good					729:732	good	729:732	good	729:732	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	2	27	theme	g	552:552	arg1	g-1					554:556	0.1, 0.2 and 0.3 g g-1	535:556	g-1	554:556	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	3	28	theme	mechanical	665:674	arg1	properties					676:685	mechanical properties	665:685	mechanical properties	665:685	Films were evaluated according to their structure, water resistance, water vapor permeability, mechanical properties and thermal behavior.					
31997346	7	29	theme	tensile	1287:1293	arg1	strength					1295:1302	tensile strength	1287:1302	tensile strength	1287:1302	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	8	30	theme	innovative	1418:1427	arg1	strategies					1429:1438	innovative strategies to valorize sunflower waxes derived from waste material	1418:1494	innovative strategies to valorize sunflower waxes derived from waste material	1418:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	1	31	theme	low-methoxyl	183:194	arg1	pectin					196:201	low-methoxyl pectin	183:201	low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil	183:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	0	32	theme	Edible	0:5	arg1	films					7:11	Edible films	0:11	Edible films	0:11	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	1	33	theme	sunflower	263:271	arg1	samples					277:283	two sunflower wax samples	259:283	two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil	259:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	2	34	theme	0.3	548:550	arg1	g-1					554:556	0.1, 0.2 and 0.3 g g-1	535:556	g-1	554:556	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	4	35	theme	material	758:765	arg1	dispersion					734:743	general, good dispersion	720:743	general, good dispersion of the lipid material	720:765	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	1	36	theme	wax	273:275	arg1	samples					277:283	two sunflower wax samples	259:283	two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil	259:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	2	37	theme	pectin	561:566	arg1	g-1					554:556	0.1, 0.2 and 0.3 g g-1	535:556	g-1	554:556	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	5	38	theme	swelling	855:862	arg1	index					864:868	lower swelling index	849:868	lower swelling index	849:868	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	2	39	theme	waxes	408:412	arg1	samples					414:420	The two sunflower waxes samples	390:420	The two sunflower waxes samples recovered	390:430	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	5	40	theme	lower	849:853	arg1	index					864:868	lower swelling index	849:868	lower swelling index	849:868	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	5	41	theme	hydrophobic	967:977	arg1	nature					979:984	the hydrophobic nature	963:984	the hydrophobic nature of waxes	963:993	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	7	42	theme	flexible	1191:1198	arg1	films					1200:1204	More resistant, rigid and less flexible films	1160:1204	More resistant, rigid and less flexible films	1160:1204	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	2	43	theme	normal	442:447	arg1	NSFW					457:460	NSFW	457:460	NSFW	457:460	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	2	43	theme	normal	442:447	arg1	hybrid					449:454	the normal hybrid	438:454	the normal hybrid	438:454	The two sunflower waxes samples recovered (from the normal hybrid, NSFW, and from the high-oleic hybrid, HOSFW) were added in three proportions (0.1, 0.2 and 0.3 g g-1 of pectin).					
31997346	1	44	theme	different	206:214	arg1	10					232:233	10	232:233	10	232:233	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	1	44	theme	different	206:214	arg1	concentrations					216:229	different concentrations	206:229	different concentrations (10, 20 and 30 g kg-1 )	206:253	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	1	44	theme	different	206:214	arg1	30 g kg-1					243:251	10, 20 and 30 g kg-1	232:251	30 g kg-1	243:251	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	6	45	theme	transfer	1025:1032	arg1	rates					1034:1038	water vapor transfer rates	1013:1038	water vapor transfer rates	1013:1038	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	46	from	reduction	1000:1008	arg1	rates					1034:1038	water vapor transfer rates	1013:1038	water vapor transfer rates	1013:1038	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	1	47	theme	sunflower	375:383	arg1	oil					385:387	sunflower oil	375:387	sunflower oil	375:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	5	48	theme	greater	914:920	arg1	content					929:935	a greater pectin content	912:935	a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes	912:993	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	6	49	theme	addition	1080:1087	arg1	effect					1062:1067	the effect	1058:1067	the effect of the wax addition, their fatty acid composition and their good distribution on the film	1058:1157	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	0	50	theme	aqueous	22:28	arg1	emulsions					30:38	aqueous emulsions	22:38	aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes	22:105	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	1	51	theme	oil	385:387	arg1	process					364:370	the winterization process	346:370	the winterization process of sunflower oil	346:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	5	52	theme	pectin	922:927	arg1	content					929:935	a greater pectin content	912:935	a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes	912:993	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	1	53	from	concentrations	216:229	arg1	pectin					196:201	low-methoxyl pectin	183:201	low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil	183:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	3	54	theme	water	621:625	arg1	resistance					627:636	water resistance	621:636	water resistance	621:636	Films were evaluated according to their structure, water resistance, water vapor permeability, mechanical properties and thermal behavior.					
31997346	0	55	theme	low-methoxyl	43:54	arg1	pectin					56:61	low-methoxyl pectin	43:61	low-methoxyl pectin	43:61	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	8	56	theme	waste	1481:1485	arg1	material					1487:1494	waste material	1481:1494	waste material	1481:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	4	57	theme	film	809:812	arg1	cross-sections					787:800	the cross-sections	783:800	the cross-sections of the film	783:812	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	5	58	theme	waxes	989:993	arg1	nature					979:984	the hydrophobic nature	963:984	the hydrophobic nature of waxes	963:993	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	5	58	theme	waxes	989:993	arg1	Ca2+					954:957	Ca2+	954:957	Ca2+	954:957	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	1	59	theme	BACKGROUND	108:117	arg1	films					126:130	BACKGROUND Edible films	108:130	BACKGROUND Edible films	108:130	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	1	60	theme	waste	304:308	arg1	samples					310:316	two waste samples	300:316	two waste samples of filter cakes produced in the winterization process of sunflower oil	300:387	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	6	61	theme	acid	1102:1105	arg1	composition					1107:1117	their fatty acid composition	1090:1117	their fatty acid composition	1090:1117	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	61	theme	acid	1102:1105	arg1	addition					1080:1087	the wax addition	1072:1087	the wax addition	1072:1087	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	8	62	attach	derived	1468:1474	arg1	material					1487:1494	waste material	1481:1494	waste material	1481:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	8	62	attach	derived	1468:1474	arg2	waxes					1462:1466	sunflower waxes	1452:1466	sunflower waxes derived from waste material	1452:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	1	63	theme	Edible	119:124	arg1	films					126:130	BACKGROUND Edible films	108:130	BACKGROUND Edible films	108:130	BACKGROUND Edible films were obtained from aqueous emulsions prepared with low-methoxyl pectin at different concentrations (10, 20 and 30 g kg-1 ) and two sunflower wax samples recovered from two waste samples of filter cakes produced in the winterization process of sunflower oil.					
31997346	3	64	theme	thermal	691:697	arg1	behavior					699:706	thermal behavior	691:706	thermal behavior	691:706	Films were evaluated according to their structure, water resistance, water vapor permeability, mechanical properties and thermal behavior.					
31997346	7	65	theme	pectin	1231:1236	arg1	content					1238:1244	lower pectin content	1225:1244	lower pectin content	1225:1244	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	6	66	theme	fatty	1096:1100	arg1	composition					1107:1117	their fatty acid composition	1090:1117	their fatty acid composition	1090:1117	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	6	66	theme	fatty	1096:1100	arg1	addition					1080:1087	the wax addition	1072:1087	the wax addition	1072:1087	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	7	67	theme	inverse	1258:1264	arg1	relationship					1266:1277	an inverse relationship	1255:1277	an inverse relationship between tensile strength and elongation percentage values	1255:1335	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	5	68	theme	water	831:835	arg1	resistance					837:846	the water resistance	827:846	the water resistance (lower swelling index and water adsorption)	827:890	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	7	69	theme	resistant	1165:1173	arg1	films					1200:1204	More resistant, rigid and less flexible films	1160:1204	More resistant, rigid and less flexible films	1160:1204	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	7	70	theme	percentage	1319:1328	arg1	values					1330:1335	elongation percentage values	1308:1335	elongation percentage values	1308:1335	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	6	71	theme	water	1013:1017	arg1	rates					1034:1038	water vapor transfer rates	1013:1038	water vapor transfer rates	1013:1038	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	5	72	dep	resistance	837:846	arg1	index					864:868	lower swelling index	849:868	lower swelling index	849:868	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	5	72	dep	resistance	837:846	arg1	adsorption					880:889	water adsorption	874:889	water adsorption	874:889	Increase in the water resistance (lower swelling index and water adsorption) was associated with a greater pectin content crosslinked with Ca2+ and the hydrophobic nature of waxes.					
31997346	6	73	theme	vapor	1019:1023	arg1	rates					1034:1038	water vapor transfer rates	1013:1038	water vapor transfer rates	1013:1038	The reduction in water vapor transfer rates was influenced by the effect of the wax addition, their fatty acid composition and their good distribution on the film.					
31997346	8	74	from	evidence	1363:1370	arg1	development					1403:1413	the development	1399:1413	the development of innovative strategies to valorize sunflower waxes derived from waste material	1399:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	7	75	theme	rigid	1176:1180	arg1	films					1200:1204	More resistant, rigid and less flexible films	1160:1204	More resistant, rigid and less flexible films	1160:1204	More resistant, rigid and less flexible films were obtained with lower pectin content, finding an inverse relationship between tensile strength and elongation percentage values.					
31997346	8	76	theme	sunflower	1452:1460	arg1	waxes					1462:1466	sunflower waxes	1452:1466	sunflower waxes derived from waste material	1452:1494	CONCLUSION These results evidence a promising alternative in the development of innovative strategies to valorize sunflower waxes derived from waste material.					
31997346	0	77	theme	pectin	56:61	arg1	emulsions					30:38	aqueous emulsions	22:38	aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes	22:105	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	4	78	theme	general	720:726	arg1	dispersion					734:743	general, good dispersion	720:743	general, good dispersion of the lipid material	720:765	RESULTS In general, good dispersion of the lipid material was observed in the cross-sections of the film.					
31997346	0	79	with	emulsions	30:38	arg1	recovered					68:76	recovered	68:76	recovered	68:76	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	0	79	with	emulsions	30:38	arg1	waxes					101:105	purified sunflower waxes	82:105	purified sunflower waxes	82:105	Edible films based on aqueous emulsions of low-methoxyl pectin with recovered and purified sunflower waxes.					
31997346	9	80	theme	Chemical	1515:1522	arg1	Industry					1524:1531	Chemical Industry	1515:1531	Chemical Industry	1515:1531	© 2020 Society of Chemical Industry.					
33682050	5	0	theme	central	823:829	arg1	design					841:846	a central composite design	821:846	a central composite design	821:846	Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate.					
33682050	9	1	theme	substrate	1313:1321	arg1	saturation					1323:1332	substrate saturation	1313:1332	substrate saturation	1313:1332	The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L.					
33682050	6	2	theme	initial	950:956	arg1	concentration					963:975	initial cell concentration	950:975	initial cell concentration of 30 g/L	950:985	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	1	3	theme	biomass	174:180	arg1	hydrolysis					144:153	hydrolysis	144:153	hydrolysis	144:153	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	1	3	theme	biomass	174:180	arg1	pretreatment					127:138	pretreatment	127:138	pretreatment	127:138	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	3	4	from	present	577:583	arg1	hydrolysate					625:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	0	5	theme	Ethanol	84:90	arg1	Production					92:101	Ethanol Production	84:101	Ethanol Production from Xylose	84:113	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	6	6	theme	g/L	983:985	arg1	concentration					963:975	initial cell concentration	950:975	initial cell concentration of 30 g/L	950:985	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	8	7	theme	kinetic	1192:1198	arg1	model					1200:1204	A kinetic model	1190:1204	A kinetic model	1190:1204	A kinetic model was developed to describe the inhibition of fermentation by substrate and product.					
33682050	11	8	theme	standard	1541:1548	arg1	deviation					1550:1558	a residual standard deviation	1530:1558	a residual standard deviation lower than 6%	1530:1572	There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.					
33682050	6	9	theme	ethanol	1037:1043	arg1	g/L					1030:1032	19.4 g/L	1025:1032	19.4 g/L of ethanol	1025:1043	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	9	10	theme	inhibition	1338:1347	arg1	constant					1349:1356	inhibition constant	1338:1356	inhibition constant	1338:1356	The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L.					
33682050	10	11	theme	Ethanol	1399:1405	arg1	concentration					1407:1419	Ethanol concentration	1399:1419	Ethanol concentration that stops cell growth	1399:1442	Ethanol concentration that stops cell growth was 30.1 g/L.					
33682050	2	12	theme	yeast	372:376	arg1	metabolism					378:387	yeast metabolism	372:387	yeast metabolism	372:387	These compounds inhibit yeast metabolism, reducing fermentation yields and productivity.					
33682050	1	13	theme	phenolic	285:292	arg1	compounds					294:302	phenolic compounds	285:302	phenolic compounds	285:302	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	11	14	theme	residual	1532:1539	arg1	deviation					1550:1558	a residual standard deviation	1530:1558	a residual standard deviation lower than 6%	1530:1572	There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.					
33682050	6	15	theme	g/L	1106:1108	arg1	rate					1094:1097	xylose consumption rate	1075:1097	xylose consumption rate	1075:1097	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	6	15	theme	g/L	1106:1108	arg1	yield					1064:1068	yield	1064:1068	yield	1064:1068	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	6	15	theme	g/L	1106:1108	arg1	productivity					1050:1061	productivity	1050:1061	productivity	1050:1061	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	4	16	theme	concentration	721:733	arg1	increments					695:704	progressive increments	683:704	progressive increments of hydrolysate concentration	683:733	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	7	17	from	h	1110:1110	arg1	sugarcane					1144:1152	a sugarcane	1142:1152	a sugarcane	1142:1152	h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate.					
33682050	6	18	theme	cell	958:961	arg1	concentration					963:975	initial cell concentration	950:975	initial cell concentration of 30 g/L	950:985	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	1	19	dep	pretreatment	127:138	arg1	the					123:125	the	123:125	the	123:125	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	4	20	theme	hydrolysate	709:719	arg1	concentration					721:733	hydrolysate concentration	709:733	hydrolysate concentration	709:733	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	5	21	theme	composite	831:839	arg1	design					841:846	a central composite design	821:846	a central composite design	821:846	Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate.					
33682050	3	22	theme	bagasse	617:623	arg1	hydrolysate					625:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	2	23	theme	fermentation	399:410	arg1	yields					412:417	fermentation yields	399:417	fermentation yields	399:417	These compounds inhibit yeast metabolism, reducing fermentation yields and productivity.					
33682050	0	24	theme	Enhanced	0:7	arg1	Tolerance					9:17	Enhanced Tolerance	0:17	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.	0:114	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	10	25	theme	cell	1432:1435	arg1	growth					1437:1442	cell growth	1432:1442	cell growth	1432:1442	Ethanol concentration that stops cell growth was 30.1 g/L.					
33682050	4	26	theme	hydrolysate	786:796	arg1	inhibitors					798:807	hydrolysate inhibitors	786:807	hydrolysate inhibitors	786:807	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	1	27	theme	rich	206:209	arg1	hydrolysate					194:204	a hydrolysate	192:204	a hydrolysate rich in fermentable sugars	192:231	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	0	28	theme	Spathaspora	22:32	arg1	passalidarum					34:45	Spathaspora passalidarum	22:45	Spathaspora passalidarum	22:45	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	5	29	theme	ethanol	874:880	arg1	production					882:891	ethanol production	874:891	ethanol production	874:891	Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate.					
33682050	3	30	theme	hemicellulosic	592:605	arg1	hydrolysate					625:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	11	31	theme	lower	1560:1564	arg1	deviation					1550:1558	a residual standard deviation	1530:1558	a residual standard deviation lower than 6%	1530:1572	There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.					
33682050	4	32	theme	progressive	683:693	arg1	increments					695:704	progressive increments	683:704	progressive increments of hydrolysate concentration	683:733	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	6	33	from	conditions	938:947	arg1	able					1009:1012	able	1009:1012	able	1009:1012	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	7	34	from	g/g	1120:1122	arg1	sugarcane					1144:1152	a sugarcane	1142:1152	a sugarcane	1142:1152	h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate.					
33682050	3	35	theme	sugarcane	607:615	arg1	hydrolysate					625:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	3	36	attach	present	577:583	arg2	inhibitors					566:575	the inhibitors	562:575	the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate	562:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	3	36	attach	present	577:583	arg1	hydrolysate					625:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	the hemicellulosic sugarcane bagasse hydrolysate	588:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	1	37	theme	fermentable	214:224	arg1	sugars					226:231	fermentable sugars	214:231	fermentable sugars	214:231	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	7	38	theme	hemicellulosic	1162:1175	arg1	hydrolysate					1177:1187	hemicellulosic hydrolysate	1162:1187	hemicellulosic hydrolysate	1162:1187	h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate.					
33682050	1	39	theme	organic	309:315	arg1	acids					317:321	organic acids	309:321	organic acids	309:321	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	6	40	theme	consumption	1082:1092	arg1	rate					1094:1097	xylose consumption rate	1075:1097	xylose consumption rate	1075:1097	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	0	41	theme	passalidarum	34:45	arg1	Tolerance					9:17	Enhanced Tolerance	0:17	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.	0:114	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	1	42	dep	furaldehydes	234:245	arg1	furfural					248:255	furfural	248:255	furfural	248:255	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	1	42	dep	furaldehydes	234:245	arg1	hydroxymethylfurfural					261:281	hydroxymethylfurfural	261:281	hydroxymethylfurfural	261:281	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	4	43	with	evolution	667:675	arg1	increments					695:704	progressive increments	683:704	progressive increments of hydrolysate concentration	683:733	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	1	44	from	sugars	226:231	arg1	rich					206:209	rich	206:209	rich	206:209	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	3	45	theme	Spathaspora	483:493	arg1	ability					472:478	the ability	468:478	the ability of Spathaspora passalidarum to ferment xylose	468:524	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	0	46	theme	Bagasse	60:66	arg1	Hydrolysate					68:78	Sugarcane Bagasse Hydrolysate	50:78	Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose	50:113	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	0	47	theme	Sugarcane	50:58	arg1	Hydrolysate					68:78	Sugarcane Bagasse Hydrolysate	50:78	Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose	50:113	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	6	48	theme	optimized	928:936	arg1	conditions					938:947	optimized conditions	928:947	optimized conditions (initial cell concentration of 30 g/L)	928:986	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	11	49	theme	experimental	1503:1514	arg1	results					1516:1522	simulated and experimental results	1489:1522	simulated and experimental results	1489:1522	There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.					
33682050	6	50	dep	conditions	938:947	arg1	concentration					963:975	initial cell concentration	950:975	initial cell concentration of 30 g/L	950:985	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	4	51	theme	laboratory	656:665	arg1	evolution					667:675	an adaptive laboratory evolution	644:675	an adaptive laboratory evolution	644:675	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	3	52	theme	present	577:583	arg1	inhibitors					566:575	the inhibitors	562:575	the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate	562:635	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	0	53	from	Xylose	108:113	arg1	Production					92:101	Ethanol Production	84:101	Ethanol Production from Xylose	84:113	Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose.					
33682050	3	54	dep	Spathaspora	483:493	arg1	passalidarum					495:506	Spathaspora passalidarum	483:506	Spathaspora passalidarum	483:506	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	4	55	theme	resistant	773:781	arg1	strain					761:766	a strain	759:766	a strain more resistant to hydrolysate inhibitors	759:807	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	11	56	theme	simulated	1489:1497	arg1	results					1516:1522	simulated and experimental results	1489:1522	simulated and experimental results	1489:1522	There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.					
33682050	4	57	theme	adaptive	647:654	arg1	evolution					667:675	an adaptive laboratory evolution	644:675	an adaptive laboratory evolution	644:675	Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors.					
33682050	6	58	theme	xylose	1075:1080	arg1	rate					1094:1097	xylose consumption rate	1075:1097	xylose consumption rate	1075:1097	At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.					
33682050	1	59	from	rich	206:209	arg1	sugars					226:231	fermentable sugars	214:231	fermentable sugars	214:231	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
33682050	3	60	from	hydrolysate	625:635	arg1	present					577:583	present	577:583	present	577:583	This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate.					
33682050	8	61	theme	fermentation	1250:1261	arg1	inhibition					1236:1245	the inhibition	1232:1245	the inhibition of fermentation by substrate and product	1232:1286	A kinetic model was developed to describe the inhibition of fermentation by substrate and product.					
33682050	1	62	theme	lignocellulosic	158:172	arg1	biomass					174:180	lignocellulosic biomass	158:180	lignocellulosic biomass	158:180	During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released.					
31915904	0	0	theme	process	76:82	arg1	evaluation					84:93	process evaluation	76:93	process evaluation	76:93	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	5	1	theme	suitable	741:748	arg1	product					733:739	a product	731:739	a product suitable for use in marine aquaculture by addition to the seawater	731:806	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	5	1	theme	suitable	741:748	arg1	possibilities					635:647	the possibilities	631:647	the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154)	631:726	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	7	2	theme	classical	979:987	arg1	procedures					989:998	classical procedures	979:998	classical procedures (freeze-drying and freezing)	979:1027	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	6	3	from	release	917:923	arg1	seawater					932:939	the seawater	928:939	the seawater	928:939	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
31915904	5	4	from	use	754:756	arg1	aquaculture					768:778	marine aquaculture	761:778	marine aquaculture	761:778	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	4	5	theme	complex	565:571	arg1	requirements					573:584	any complex requirements	561:584	any complex requirements for use or storage	561:603	In addition, only low-cost technologies are sustainable for this industry, without any complex requirements for use or storage.					
31915904	3	6	theme	many	370:373	arg1	cases					375:379	many cases	370:379	many cases	370:379	In many cases, the bacteria are released in the seawater, where they have to be at appropriate concentrations.					
31915904	4	7	theme	low-cost	496:503	arg1	technologies					505:516	only low-cost technologies	491:516	only low-cost technologies	491:516	In addition, only low-cost technologies are sustainable for this industry, without any complex requirements for use or storage.					
31915904	8	8	from	immobilization	1200:1213	arg1	beads					1258:1262	calcium alginate beads	1241:1262	calcium alginate beads	1241:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	6	9	theme	marine	866:871	arg1	bacteria					873:880	the viable marine bacteria	855:880	the viable marine bacteria	855:880	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
31915904	7	10	theme	undesirable	1052:1062	arg1	results					1064:1070	undesirable results	1052:1070	undesirable results of the interaction of the products obtained with natural seawater	1052:1136	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	6	11	from	method	811:816	arg1	seawater					932:939	the seawater	928:939	the seawater	928:939	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
31915904	0	12	theme	surface	110:116	arg1	methodology					118:128	response surface methodology	101:128	response surface methodology	101:128	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	0	13	from	use	42:44	arg1	facilities					61:70	aquaculture facilities	49:70	aquaculture facilities	49:70	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	4	14	from	addition	481:488	arg1	sustainable					522:532	sustainable	522:532	sustainable	522:532	In addition, only low-cost technologies are sustainable for this industry, without any complex requirements for use or storage.					
31915904	8	15	theme	immobilization	1200:1213	arg1	results					1185:1191	the results	1181:1191	the results of the immobilization of the marine bacteria in calcium alginate beads	1181:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	9	16	dep	hardness	1428:1435	arg1	retain					1440:1445	retain	1440:1445	to retain the bacteria	1437:1458	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	9	16	dep	hardness	1428:1435	arg1	released					1477:1484	released	1477:1484	to be easily released in the marine aquaculture environment	1464:1522	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	1	17	theme	marine	168:173	arg1	aquaculture					175:185	marine aquaculture	168:185	marine aquaculture	168:185	New strategies are being proposed in marine aquaculture to use marine bacteria as alternative to antibiotics, as nutritional additive or as immune-stimulant.					
31915904	0	18	theme	response	101:108	arg1	methodology					118:128	response surface methodology	101:128	response surface methodology	101:128	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	10	19	theme	surface	1624:1630	arg1	methodology					1632:1642	a standard response surface methodology	1604:1642	a standard response surface methodology (RSM)	1604:1648	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	19	theme	surface	1624:1630	arg1	design					1589:1594	the central composite rotatable design	1557:1594	the central composite rotatable design (CCRD)	1557:1601	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	19	theme	surface	1624:1630	arg1	RSM					1645:1647	RSM	1645:1647	RSM	1645:1647	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	20	theme	response	1615:1622	arg1	methodology					1632:1642	a standard response surface methodology	1604:1642	a standard response surface methodology (RSM)	1604:1648	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	20	theme	response	1615:1622	arg1	design					1589:1594	the central composite rotatable design	1557:1594	the central composite rotatable design (CCRD)	1557:1601	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	20	theme	response	1615:1622	arg1	RSM					1645:1647	RSM	1645:1647	RSM	1645:1647	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	7	21	dep	procedures	989:998	arg1	freezing					1019:1026	freezing	1019:1026	freezing	1019:1026	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	7	21	dep	procedures	989:998	arg1	freeze-drying					1001:1013	freeze-drying	1001:1013	freeze-drying	1001:1013	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	9	22	theme	final	1269:1273	arg1	product					1275:1281	The final product	1265:1281	The final product	1265:1281	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	9	23	theme	marine	1493:1498	arg1	environment					1512:1522	the marine aquaculture environment	1489:1522	the marine aquaculture environment	1489:1522	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	6	24	theme	viable	859:864	arg1	bacteria					873:880	the viable marine bacteria	855:880	the viable marine bacteria	855:880	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
31915904	7	25	theme	natural	1121:1127	arg1	seawater					1129:1136	natural seawater	1121:1136	natural seawater	1121:1136	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	2	26	dep	larval	337:342	arg1	cultures					357:364	cultures	357:364	cultures	357:364	These approaches are particularly promising for larval and juvenile cultures.					
31915904	9	27	theme	beads	1406:1410	arg1	formation					1393:1401	the formation	1389:1401	the formation of beads	1389:1410	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	0	28	theme	marine	22:27	arg1	bacteria					29:36	live marine bacteria	17:36	live marine bacteria	17:36	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	5	29	theme	marine	680:685	arg1	Phaeobacter					708:718	Phaeobacter	708:718	Phaeobacter	708:718	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	5	29	theme	marine	680:685	arg1	bacterium					697:705	a potential marine probiotic bacterium	668:705	a potential marine probiotic bacterium (Phaeobacter PP-154)	668:726	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	8	30	from	beads	1258:1262	arg1	results					1185:1191	the results	1181:1191	the results of the immobilization of the marine bacteria in calcium alginate beads	1181:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	1	31	theme	marine	194:199	arg1	bacteria					201:208	marine bacteria	194:208	marine bacteria	194:208	New strategies are being proposed in marine aquaculture to use marine bacteria as alternative to antibiotics, as nutritional additive or as immune-stimulant.					
31915904	0	32	theme	live	17:20	arg1	bacteria					29:36	live marine bacteria	17:36	live marine bacteria	17:36	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	5	33	theme	probiotic	687:695	arg1	Phaeobacter					708:718	Phaeobacter	708:718	Phaeobacter	708:718	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	5	33	theme	probiotic	687:695	arg1	bacterium					697:705	a potential marine probiotic bacterium	668:705	a potential marine probiotic bacterium (Phaeobacter PP-154)	668:726	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	9	34	theme	aquaculture	1500:1510	arg1	environment					1512:1522	the marine aquaculture environment	1489:1522	the marine aquaculture environment	1489:1522	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	3	35	theme	appropriate	450:460	arg1	concentrations					462:475	appropriate concentrations	450:475	appropriate concentrations	450:475	In many cases, the bacteria are released in the seawater, where they have to be at appropriate concentrations.					
31915904	10	36	theme	composite	1569:1577	arg1	methodology					1632:1642	a standard response surface methodology	1604:1642	a standard response surface methodology (RSM)	1604:1648	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	36	theme	composite	1569:1577	arg1	CCRD					1597:1600	CCRD	1597:1600	CCRD	1597:1600	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	36	theme	composite	1569:1577	arg1	design					1589:1594	the central composite rotatable design	1557:1594	the central composite rotatable design (CCRD)	1557:1601	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	5	37	theme	bacterium	697:705	arg1	preservation					652:663	preservation	652:663	preservation of a potential marine probiotic bacterium (Phaeobacter PP-154)	652:726	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	10	38	theme	rotatable	1579:1587	arg1	methodology					1632:1642	a standard response surface methodology	1604:1642	a standard response surface methodology (RSM)	1604:1648	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	38	theme	rotatable	1579:1587	arg1	CCRD					1597:1600	CCRD	1597:1600	CCRD	1597:1600	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	38	theme	rotatable	1579:1587	arg1	design					1589:1594	the central composite rotatable design	1557:1594	the central composite rotatable design (CCRD)	1557:1601	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	8	39	theme	marine	1222:1227	arg1	bacteria					1229:1236	the marine bacteria	1218:1236	the marine bacteria	1218:1236	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	0	40	theme	bacteria	29:36	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation	0:93	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	6	41	theme	rapid	911:915	arg1	release					917:923	their rapid release	905:923	their rapid release in the seawater	905:939	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
31915904	10	42	theme	central	1561:1567	arg1	methodology					1632:1642	a standard response surface methodology	1604:1642	a standard response surface methodology (RSM)	1604:1648	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	42	theme	central	1561:1567	arg1	CCRD					1597:1600	CCRD	1597:1600	CCRD	1597:1600	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	42	theme	central	1561:1567	arg1	design					1589:1594	the central composite rotatable design	1557:1594	the central composite rotatable design (CCRD)	1557:1601	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	7	43	theme	products	1098:1105	arg1	interaction					1079:1089	the interaction	1075:1089	the interaction of the products obtained with natural seawater	1075:1136	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	7	44	theme	previous	964:971	arg1	step					973:976	a previous step	962:976	a previous step	962:976	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	9	45	theme	balanced	1419:1426	arg1	hardness					1428:1435	a balanced hardness	1417:1435	a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment	1417:1522	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	5	46	theme	marine	761:766	arg1	aquaculture					768:778	marine aquaculture	761:778	marine aquaculture	761:778	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	5	47	theme	preservation	652:663	arg1	product					733:739	a product	731:739	a product suitable for use in marine aquaculture by addition to the seawater	731:806	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	5	47	theme	preservation	652:663	arg1	possibilities					635:647	the possibilities	631:647	the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154)	631:726	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	0	48	theme	aquaculture	49:59	arg1	facilities					61:70	aquaculture facilities	49:70	aquaculture facilities	49:70	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	6	49	theme	bacteria	873:880	arg1	preservation					839:850	the preservation	835:850	the preservation of the viable marine bacteria	835:880	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
31915904	8	50	from	results	1185:1191	arg1	beads					1258:1262	calcium alginate beads	1241:1262	calcium alginate beads	1241:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	8	51	theme	bacteria	1229:1236	arg1	immobilization					1200:1213	the immobilization	1196:1213	the immobilization of the marine bacteria in calcium alginate beads	1196:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	9	52	with	interference	1359:1370	arg1	alginate					1377:1384	alginate	1377:1384	alginate	1377:1384	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	7	53	theme	interaction	1079:1089	arg1	results					1064:1070	undesirable results	1052:1070	undesirable results of the interaction of the products obtained with natural seawater	1052:1136	In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives.					
31915904	1	54	theme	New	131:133	arg1	strategies					135:144	New strategies	131:144	New strategies	131:144	New strategies are being proposed in marine aquaculture to use marine bacteria as alternative to antibiotics, as nutritional additive or as immune-stimulant.					
31915904	5	55	theme	potential	670:678	arg1	Phaeobacter					708:718	Phaeobacter	708:718	Phaeobacter	708:718	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	5	55	theme	potential	670:678	arg1	bacterium					697:705	a potential marine probiotic bacterium	668:705	a potential marine probiotic bacterium (Phaeobacter PP-154)	668:726	In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater.					
31915904	8	56	theme	calcium	1241:1247	arg1	beads					1258:1262	calcium alginate beads	1241:1262	calcium alginate beads	1241:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	8	57	theme	alginate	1249:1256	arg1	beads					1258:1262	calcium alginate beads	1241:1262	calcium alginate beads	1241:1262	We report the results of the immobilization of the marine bacteria in calcium alginate beads.					
31915904	0	58	from	evaluation	84:93	arg1	facilities					61:70	aquaculture facilities	49:70	aquaculture facilities	49:70	Encapsulation of live marine bacteria for use in aquaculture facilities and process evaluation using response surface methodology.					
31915904	10	59	theme	standard	1606:1613	arg1	methodology					1632:1642	a standard response surface methodology	1604:1642	a standard response surface methodology (RSM)	1604:1648	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	59	theme	standard	1606:1613	arg1	design					1589:1594	the central composite rotatable design	1557:1594	the central composite rotatable design (CCRD)	1557:1601	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	10	59	theme	standard	1606:1613	arg1	RSM					1645:1647	RSM	1645:1647	RSM	1645:1647	The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).					
31915904	9	60	theme	bacteria	1342:1349	arg1	requirements					1322:1333	the requirements	1318:1333	the requirements of the bacteria	1318:1349	The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment.					
31915904	4	61	from	sustainable	522:532	arg1	addition					481:488	addition	481:488	addition	481:488	In addition, only low-cost technologies are sustainable for this industry, without any complex requirements for use or storage.					
31915904	6	62	theme	saline	887:892	arg1	medium					894:899	a saline medium	885:899	a saline medium	885:899	A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for.					
33217460	1	0	theme	cassia	294:299	arg1	gum					301:303	cassia gum	294:303	cassia gum (CG)	294:308	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	1	0	theme	cassia	294:299	arg1	CG					306:307	CG	306:307	CG	306:307	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	9	1	contain	had	1280:1282	arg2	activity					1307:1314	superior antioxidative activity	1284:1314	superior antioxidative activity	1284:1314	These results showed that the CG/FSIE film had superior antioxidative activity compared to films made from plastic and pure CG.					
33217460	9	1	contain	had	1280:1282	arg1	film					1275:1278	the CG/FSIE film	1263:1278	the CG/FSIE film	1263:1278	These results showed that the CG/FSIE film had superior antioxidative activity compared to films made from plastic and pure CG.					
33217460	9	2	theme	antioxidative	1293:1305	arg1	activity					1307:1314	superior antioxidative activity	1284:1314	superior antioxidative activity	1284:1314	These results showed that the CG/FSIE film had superior antioxidative activity compared to films made from plastic and pure CG.					
33217460	9	3	theme	CG/FSIE	1267:1273	arg1	film					1275:1278	the CG/FSIE film	1263:1278	the CG/FSIE film	1263:1278	These results showed that the CG/FSIE film had superior antioxidative activity compared to films made from plastic and pure CG.					
33217460	7	4	theme	practical	954:962	arg1	application					964:974	The practical application	950:974	The practical application of these composite films	950:999	The practical application of these composite films was investigated by evaluating the quality of lard wrapped in the films.					
33217460	6	5	theme	ethanol	941:947	arg1	ethanol					941:947	ethanol	941:947	ethanol	941:947	Total phenols exhibited a positive trend as the amount of FSIE increased in 50% of ethanol.					
33217460	6	5	theme	ethanol	941:947	arg1	%					936:936	50%	934:936	50% of ethanol	934:947	Total phenols exhibited a positive trend as the amount of FSIE increased in 50% of ethanol.					
33217460	4	6	theme	appropriate	597:607	arg1	FSIE					620:623	FSIE	620:623	FSIE	620:623	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	4	6	theme	appropriate	597:607	arg1	amounts					609:615	The appropriate amounts	593:615	The appropriate amounts of FSIE	593:623	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	1	7	theme	Flos	242:245	arg1	FSIE					275:278	FSIE	275:278	FSIE	275:278	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	1	7	theme	Flos	242:245	arg1	extract					266:272	the Flos Sophorae Immaturus extract	238:272	the Flos Sophorae Immaturus extract (FSIE)	238:279	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	7	8	theme	lard	1047:1050	arg1	quality					1036:1042	the quality	1032:1042	the quality of lard wrapped in the films	1032:1071	The practical application of these composite films was investigated by evaluating the quality of lard wrapped in the films.					
33217460	9	9	theme	superior	1284:1291	arg1	activity					1307:1314	superior antioxidative activity	1284:1314	superior antioxidative activity	1284:1314	These results showed that the CG/FSIE film had superior antioxidative activity compared to films made from plastic and pure CG.					
33217460	8	10	theme	lard	1135:1138	arg1	POV					1127:1129	POV	1127:1129	POV	1127:1129	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	10	theme	lard	1135:1138	arg1	XAV					1102:1104	XAV	1102:1104	XAV	1102:1104	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	10	theme	lard	1135:1138	arg1	value					1095:1099	the acid value	1086:1099	the acid value (XAV)	1086:1105	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	10	theme	lard	1135:1138	arg1	value					1120:1124	peroxide value	1111:1124	peroxide value (POV)	1111:1130	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	10	theme	lard	1135:1138	arg1	lower					1167:1171	lower	1167:1171	lower	1167:1171	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	9	11	theme	pure	1356:1359	arg1	CG					1361:1362	pure CG	1356:1362	pure CG	1356:1362	These results showed that the CG/FSIE film had superior antioxidative activity compared to films made from plastic and pure CG.					
33217460	0	12	theme	antioxidant	84:94	arg1	properties					120:129	the antioxidant, mechanical and barrier properties	80:129	properties	120:129	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	4	13	theme	27.6	742:745	arg1	%					737:737	%	737:737	%	737:737	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	1	14	theme	Immaturus	256:264	arg1	FSIE					275:278	FSIE	275:278	FSIE	275:278	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	1	14	theme	Immaturus	256:264	arg1	extract					266:272	the Flos Sophorae Immaturus extract	238:272	the Flos Sophorae Immaturus extract (FSIE)	238:279	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	4	15	theme	FSIE	620:623	arg1	FSIE					620:623	FSIE	620:623	FSIE	620:623	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	4	15	theme	FSIE	620:623	arg1	amounts					609:615	The appropriate amounts	593:615	The appropriate amounts of FSIE	593:623	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	5	16	theme	free	833:836	arg1	scavenging					846:855	free radical scavenging	833:855	free radical scavenging	833:855	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	0	17	theme	mechanical	97:106	arg1	properties					120:129	the antioxidant, mechanical and barrier properties	80:129	properties	120:129	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	5	18	theme	radical	838:844	arg1	scavenging					846:855	free radical scavenging	833:855	free radical scavenging	833:855	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	1	19	theme	novel	155:159	arg1	film					175:178	A novel antioxidative film	153:178	A novel antioxidative film	153:178	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	4	20	theme	38.7	733:736	arg1	%					737:737	%	737:737	%	737:737	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	5	21	theme	antioxidative	792:804	arg1	activity					806:813	strong antioxidative activity	785:813	strong antioxidative activity	785:813	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	1	22	theme	antioxidative	161:173	arg1	film					175:178	A novel antioxidative film	153:178	A novel antioxidative film	153:178	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	0	23	theme	Self-assembling	0:14	arg1	crystals					16:23	Self-assembling crystals	0:23	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.	0:151	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	3	24	theme	ultraviolet	560:570	arg1	light					572:576	ultraviolet light	560:576	ultraviolet light	560:576	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	4	25	theme	tensile	639:645	arg1	TS					657:658	TS	657:658	TS	657:658	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	4	25	theme	tensile	639:645	arg1	strength					647:654	the tensile strength	635:654	the tensile strength (TS)	635:659	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	2	26	theme	electron	320:327	arg1	SEM					341:343	SEM	341:343	SEM	341:343	Scanning electron microscopy (SEM) showed that FSIE was successfully compounded with CG.					
33217460	2	26	theme	electron	320:327	arg1	microscopy					329:338	Scanning electron microscopy	311:338	Scanning electron microscopy (SEM)	311:344	Scanning electron microscopy (SEM) showed that FSIE was successfully compounded with CG.					
33217460	8	27	theme	acid	1090:1093	arg1	XAV					1102:1104	XAV	1102:1104	XAV	1102:1104	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	27	theme	acid	1090:1093	arg1	value					1095:1099	the acid value	1086:1099	the acid value (XAV)	1086:1105	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	27	theme	acid	1090:1093	arg1	lower					1167:1171	lower	1167:1171	lower	1167:1171	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	28	theme	CG/FSIE2	1152:1159	arg1	%					1160:1160	CG/FSIE2%	1152:1160	CG/FSIE2%	1152:1160	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	0	29	theme	barrier	112:118	arg1	properties					120:129	the antioxidant, mechanical and barrier properties	80:129	properties	120:129	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	5	30	theme	high	819:822	arg1	rates					824:828	high rates	819:828	high rates of free radical scavenging	819:855	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	3	31	theme	water	453:457	arg1	WVP					479:481	WVP	479:481	WVP	479:481	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	3	31	theme	water	453:457	arg1	permeability					465:476	the water vapor permeability	449:476	the water vapor permeability (WVP)	449:482	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	7	32	theme	films	995:999	arg1	application					964:974	The practical application	950:974	The practical application of these composite films	950:999	The practical application of these composite films was investigated by evaluating the quality of lard wrapped in the films.					
33217460	1	33	contain	containing	227:236	arg1	solution					218:225	a film-forming solution	203:225	drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%)	196:288	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	1	33	contain	containing	227:236	arg2	extract					266:272	the Flos Sophorae Immaturus extract	238:272	the Flos Sophorae Immaturus extract (FSIE)	238:279	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	1	33	contain	containing	227:236	arg2	FSIE					275:278	FSIE	275:278	FSIE	275:278	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	4	34	dep	30.2 MPa	678:685	arg1	20.9 MPa					666:673	20.9 MPa	666:673	20.9 MPa	666:673	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	0	35	theme	extract	31:37	arg1	crystals					16:23	Self-assembling crystals	0:23	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.	0:151	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	6	36	theme	Total	858:862	arg1	phenols					864:870	Total phenols	858:870	Total phenols	858:870	Total phenols exhibited a positive trend as the amount of FSIE increased in 50% of ethanol.					
33217460	6	37	theme	FSIE	916:919	arg1	amount					906:911	the amount	902:911	the amount of FSIE	902:919	Total phenols exhibited a positive trend as the amount of FSIE increased in 50% of ethanol.					
33217460	6	37	theme	FSIE	916:919	arg1	FSIE					916:919	FSIE	916:919	FSIE	916:919	Total phenols exhibited a positive trend as the amount of FSIE increased in 50% of ethanol.					
33217460	0	38	theme	gum	143:145	arg1	film					147:150	a cassia gum film	134:150	a cassia gum film	134:150	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	8	39	theme	plastic	1224:1230	arg1	bag					1232:1234	plastic bag	1224:1234	plastic bag	1224:1234	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	3	40	theme	FSIE	425:428	arg1	addition					413:420	the addition	409:420	the addition of FSIE	409:428	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	4	41	dep	%	737:737	arg1	to					739:740	to	739:740	to	739:740	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	0	42	theme	cassia	136:141	arg1	film					147:150	a cassia gum film	134:150	a cassia gum film	134:150	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	7	43	theme	composite	985:993	arg1	films					995:999	these composite films	979:999	these composite films	979:999	The practical application of these composite films was investigated by evaluating the quality of lard wrapped in the films.					
33217460	5	44	theme	scavenging	846:855	arg1	activity					806:813	strong antioxidative activity	785:813	strong antioxidative activity	785:813	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	5	44	theme	scavenging	846:855	arg1	rates					824:828	high rates	819:828	high rates of free radical scavenging	819:855	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	6	45	theme	positive	884:891	arg1	trend					893:897	a positive trend	882:897	a positive trend	882:897	Total phenols exhibited a positive trend as the amount of FSIE increased in 50% of ethanol.					
33217460	8	46	theme	peroxide	1111:1118	arg1	POV					1127:1129	POV	1127:1129	POV	1127:1129	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	8	46	theme	peroxide	1111:1118	arg1	value					1120:1124	peroxide value	1111:1124	peroxide value (POV)	1111:1130	After 25 d, the acid value (XAV) and peroxide value (POV) of lard packaged in CG/FSIE2% were lower than the values for lard packaged in CG/FSIE0% and plastic bag.					
33217460	0	47	theme	film	147:150	arg1	properties					120:129	the antioxidant, mechanical and barrier properties	80:129	properties	120:129	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	1	48	dep	drying	196:201	arg1	solution					218:225	a film-forming solution	203:225	drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%)	196:288	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	5	49	theme	strong	785:790	arg1	activity					806:813	strong antioxidative activity	785:813	strong antioxidative activity	785:813	The films doped with FSIE exhibited strong antioxidative activity and high rates of free radical scavenging.					
33217460	1	50	theme	film-forming	205:216	arg1	solution					218:225	a film-forming solution	203:225	drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%)	196:288	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	1	51	dep	Flos	242:245	arg1	Sophorae					247:254	Sophorae	247:254	Sophorae	247:254	A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG).					
33217460	3	52	theme	film	516:519	arg1	WVP					479:481	WVP	479:481	WVP	479:481	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	3	52	theme	film	516:519	arg1	permeability					465:476	the water vapor permeability	449:476	the water vapor permeability (WVP)	449:482	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	3	52	theme	film	516:519	arg1	permeability					491:502	O2 permeability	488:502	O2 permeability (OP) of the film	488:519	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	3	52	theme	film	516:519	arg1	OP					505:506	OP	505:506	OP	505:506	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	2	53	theme	Scanning	311:318	arg1	SEM					341:343	SEM	341:343	SEM	341:343	Scanning electron microscopy (SEM) showed that FSIE was successfully compounded with CG.					
33217460	2	53	theme	Scanning	311:318	arg1	microscopy					329:338	Scanning electron microscopy	311:338	Scanning electron microscopy (SEM)	311:344	Scanning electron microscopy (SEM) showed that FSIE was successfully compounded with CG.					
33217460	0	54	theme	Immaturus	56:64	arg1	extract					31:37	an extract	28:37	an extract of Flos Sophorae Immaturus	28:64	Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.					
33217460	4	55	from	%	746:746	arg1	EB					724:725	EB	724:725	EB	724:725	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	4	55	from	%	746:746	arg1	break					717:721	break	717:721	break (EB) from 38.7% to 27.6%	717:746	The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%.					
33217460	3	56	theme	vapor	459:463	arg1	WVP					479:481	WVP	479:481	WVP	479:481	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33217460	3	56	theme	vapor	459:463	arg1	permeability					465:476	the water vapor permeability	449:476	the water vapor permeability (WVP)	449:482	Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly.					
33020456	3	0	theme	chemical	691:698	arg1	interactions					713:724	chemical and physical interactions	691:724	chemical and physical interactions	691:724	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	2	1	theme	maleic	556:561	arg1	acid					563:566	maleic acid	556:566	maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	556:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	1	2	theme	seawater	239:246	arg1	technology					261:270	seawater desalination technology	239:270	seawater desalination technology	239:270	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	2	3	theme	layer	471:475	arg1	deposition					434:443	the deposition	430:443	the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	430:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	4	4	theme	fibrous	860:866	arg1	structure					868:876	the fibrous structure	856:876	the fibrous structure of the active layers	856:897	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	1	5	theme	desalination	248:259	arg1	technology					261:270	seawater desalination technology	239:270	seawater desalination technology	239:270	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	0	6	theme	Reverse	91:97	arg1	Desalination					107:118	Reverse Osmosis Desalination	91:118	Reverse Osmosis Desalination	91:118	Novel Maleic Acid, Crosslinked, Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination.					
33020456	3	7	from	present	779:785	arg1	NF-TFC					794:799	the NF-TFC	790:799	the NF-TFC	790:799	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	2	8	theme	acetate	626:632	arg1	substrate					634:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	5	9	theme	NF-TFC	957:962	arg1	membranes					964:972	NF-TFC membranes	957:972	NF-TFC membranes	957:972	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	5	10	theme	desalination	925:936	arg1	characteristics					938:952	The reverse osmosis (RO) desalination characteristics	900:952	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes	900:972	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	3	11	theme	interactions	713:724	arg1	development					676:686	the development	672:686	the development of chemical and physical interactions	672:724	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	2	12	theme	cellulose	616:624	arg1	substrate					634:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	5	13	theme	membranes	964:972	arg1	characteristics					938:952	The reverse osmosis (RO) desalination characteristics	900:952	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes	900:972	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	6	14	theme	optimal	1095:1101	arg1	rejection					1108:1116	an optimal salt rejection	1092:1116	an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h	1092:1159	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	2	15	theme	chitosan	491:498	arg1	blend					482:486	a blend	480:486	a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	480:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	2	16	theme	3-triethoxysilylpropylamine	573:599	arg1	substrate					634:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	2	17	theme	functionalized	601:614	arg1	substrate					634:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	5	18	from	concentration	1005:1017	arg1	blend					1050:1054	a CS/PVP blend	1041:1054	a CS/PVP blend	1041:1054	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	0	19	theme	Osmosis	99:105	arg1	Desalination					107:118	Reverse Osmosis Desalination	91:118	Reverse Osmosis Desalination	91:118	Novel Maleic Acid, Crosslinked, Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination.					
33020456	6	20	theme	seawater	1222:1229	arg1	desalination					1231:1242	seawater desalination	1222:1242	seawater desalination	1222:1242	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	2	21	dep	novel	363:367	arg1	nanofibrous					370:380	nanofibrous	370:380	nanofibrous	370:380	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	2	21	dep	novel	363:367	arg1	composite					393:401	composite	393:401	composite	393:401	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	0	22	theme	Maleic	6:11	arg1	Acid					13:16	Novel Maleic Acid	0:16	Novel Maleic Acid	0:16	Novel Maleic Acid, Crosslinked, Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination.					
33020456	3	23	theme	physical	704:711	arg1	interactions					713:724	chemical and physical interactions	691:724	chemical and physical interactions	691:724	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	2	24	theme	desalination	320:331	arg1	process					333:339	an efficient desalination process	307:339	an efficient desalination process	307:339	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	4	25	theme	active	885:890	arg1	layers					892:897	the active layers	881:897	the active layers	881:897	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	6	26	theme	acetate	1067:1073	arg1	-S4					1079:1081	Cellulose acetate (CA)-S4	1057:1081	Cellulose acetate (CA)-S4	1057:1081	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	0	27	theme	Novel	0:4	arg1	Acid					13:16	Novel Maleic Acid	0:16	Novel Maleic Acid	0:16	Novel Maleic Acid, Crosslinked, Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination.					
33020456	1	28	dep	depleting	159:167	arg1	so					236:237	so	236:237	so	236:237	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	1	29	theme	serious	184:190	arg1	problem					192:198	a serious problem	182:198	a serious problem	182:198	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	1	30	theme	global	285:290	arg1	attention					292:300	global attention	285:300	global attention	285:300	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	0	31	dep	Vinylpyrrolidone	59:74	arg1	Acid					13:16	Novel Maleic Acid	0:16	Novel Maleic Acid	0:16	Novel Maleic Acid, Crosslinked, Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination.					
33020456	0	31	dep	Vinylpyrrolidone	59:74	arg1	Membranes					77:85	Membranes	77:85	Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination	32:118	Novel Maleic Acid, Crosslinked, Nanofibrous Chitosan/Poly (Vinylpyrrolidone) Membranes for Reverse Osmosis Desalination.					
33020456	2	32	theme	efficient	310:318	arg1	process					333:339	an efficient desalination process	307:339	an efficient desalination process	307:339	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	2	33	theme	poly	509:512	arg1	blend					482:486	a blend	480:486	a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	480:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	2	34	theme	active	464:469	arg1	layer					471:475	the nanofibrous active layer	448:475	the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	448:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	4	35	theme	microscopy	820:829	arg1	micrographs					837:847	Scanning electron microscopy (SEM) micrographs	802:847	Scanning electron microscopy (SEM) micrographs	802:847	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	6	36	theme	Cellulose	1057:1065	arg1	acetate					1067:1073	Cellulose acetate	1057:1073	Cellulose acetate (CA)-S4	1057:1081	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	6	36	theme	Cellulose	1057:1065	arg1	CA					1076:1077	CA	1076:1077	CA	1076:1077	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	1	37	theme	rapid	205:209	arg1	increases					211:219	rapid increases	205:219	rapid increases in population	205:233	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	2	38	theme	nanofibrous	452:462	arg1	layer					471:475	the nanofibrous active layer	448:475	the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	448:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	3	39	theme	present	779:785	arg1	groups					772:777	functional groups	761:777	functional groups present in the NF-TFC	761:799	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	4	40	theme	electron	811:818	arg1	microscopy					820:829	Scanning electron microscopy	802:829	Scanning electron microscopy (SEM) micrographs	802:847	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	4	40	theme	electron	811:818	arg1	SEM					832:834	SEM	832:834	SEM	832:834	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	2	41	from	acid	563:566	arg1	substrate					634:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	571:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	4	42	theme	layers	892:897	arg1	structure					868:876	the fibrous structure	856:876	the fibrous structure of the active layers	856:897	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	5	43	theme	crosslinker	1026:1036	arg1	concentration					1005:1017	the concentration	1001:1017	the concentration of the crosslinker in a CS/PVP blend	1001:1054	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	1	44	from	increases	211:219	arg1	population					224:233	population	224:233	population	224:233	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	5	45	theme	reverse	904:910	arg1	osmosis					912:918	reverse osmosis	904:918	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes	900:972	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	5	45	theme	reverse	904:910	arg1	RO					921:922	RO	921:922	RO	921:922	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	3	46	theme	functional	761:770	arg1	groups					772:777	functional groups	761:777	functional groups present in the NF-TFC	761:799	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	3	47	from	NF-TFC	794:799	arg1	present					779:785	present	779:785	present	779:785	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	6	48	theme	NF-TFC	1182:1187	arg1	membranes					1189:1197	the NF-TFC membranes	1178:1197	the NF-TFC membranes	1178:1197	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	6	48	theme	NF-TFC	1182:1187	arg1	favorable					1208:1216	favorable	1208:1216	favorable	1208:1216	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	5	49	theme	osmosis	912:918	arg1	characteristics					938:952	The reverse osmosis (RO) desalination characteristics	900:952	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes	900:972	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	3	50	theme	groups	772:777	arg1	incorporation					744:756	the incorporation	740:756	the incorporation of functional groups present in the NF-TFC	740:799	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	5	51	theme	CS/PVP	1043:1048	arg1	blend					1050:1054	a CS/PVP blend	1041:1054	a CS/PVP blend	1041:1054	The reverse osmosis (RO) desalination characteristics of NF-TFC membranes are elevated by increasing the concentration of the crosslinker in a CS/PVP blend.					
33020456	6	52	theme	salt	1103:1106	arg1	rejection					1108:1116	an optimal salt rejection	1092:1116	an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h	1092:1159	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	6	53	theme	%	1125:1125	arg1	rejection					1108:1116	an optimal salt rejection	1092:1116	an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h	1092:1159	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	3	54	attach	present	779:785	arg2	groups					772:777	functional groups	761:777	functional groups present in the NF-TFC	761:799	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	3	54	attach	present	779:785	arg1	NF-TFC					794:799	the NF-TFC	790:799	the NF-TFC	790:799	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	6	55	theme	flux	1142:1145	arg1	rejection					1108:1116	an optimal salt rejection	1092:1116	an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h	1092:1159	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	6	56	theme	L/m2h	1155:1159	arg1	%					1125:1125	98.3%	1121:1125	98.3%	1121:1125	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	6	56	theme	L/m2h	1155:1159	arg1	flux					1142:1145	permeation flux	1131:1145	permeation flux	1131:1145	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	6	56	theme	L/m2h	1155:1159	arg1	L/m2h					1155:1159	42.9 L/m2h	1150:1159	42.9 L/m2h	1150:1159	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	1	57	theme	clean	131:135	arg1	water					137:141	clean water	131:141	clean water	131:141	Fresh and clean water is consistently depleting and becoming a serious problem with rapid increases in population, so seawater desalination technology has captured global attention.					
33020456	2	58	theme	blend	482:486	arg1	layer					471:475	the nanofibrous active layer	448:475	the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	448:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	3	59	theme	FTIR	645:648	arg1	analysis					650:657	FTIR analysis	645:657	FTIR analysis	645:657	FTIR analysis demonstrated the development of chemical and physical interactions and confirmed the incorporation of functional groups present in the NF-TFC.					
33020456	4	60	theme	Scanning	802:809	arg1	microscopy					820:829	Scanning electron microscopy	802:829	Scanning electron microscopy (SEM) micrographs	802:847	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	4	60	theme	Scanning	802:809	arg1	SEM					832:834	SEM	832:834	SEM	832:834	Scanning electron microscopy (SEM) micrographs depict the fibrous structure of the active layers.					
33020456	6	61	theme	permeation	1131:1140	arg1	flux					1142:1145	permeation flux	1131:1145	permeation flux	1131:1145	Cellulose acetate (CA)-S4 attained an optimal salt rejection of 98.3% and permeation flux of 42.9 L/m2h, suggesting that the NF-TFC membranes could be favorable for seawater desalination.					
33020456	2	62	theme	novel	363:367	arg1	NF-TFC					413:418	NF-TFC	413:418	NF-TFC	413:418	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
33020456	2	62	theme	novel	363:367	arg1	membrane					403:410	a novel, nanofibrous, thin-film composite membrane	361:410	a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate	361:642	For an efficient desalination process, this work proposes a novel, nanofibrous, thin-film composite membrane (NF-TFC) based on the deposition of the nanofibrous active layer of a blend of chitosan (CS) and poly (vinylpyrrolidone) (PVP) crosslinked with maleic acid on a 3-triethoxysilylpropylamine functionalized cellulose acetate substrate.					
32145429	4	0	theme	silicone-based	777:790	arg1	phase					801:805	a silicone-based external phase	775:805	a silicone-based external phase	775:805	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	5	1	with	particles	938:946	arg1	surface					976:982	the smooth and regular surface	953:982	surface	976:982	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	6	2	theme	pig	1157:1159	arg1	skin					1166:1169	pig ears skin	1157:1169	pig ears skin	1157:1169	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	7	3	theme	murine	1301:1306	arg1	model					1322:1326	murine leishmaniasis model	1301:1326	murine leishmaniasis model	1301:1326	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	2	4	theme	cutaneous	296:304	arg1	CL					321:322	CL	321:322	CL	321:322	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	2	4	theme	cutaneous	296:304	arg1	leishmaniasis					306:318	cutaneous leishmaniasis	296:318	cutaneous leishmaniasis (CL)	296:323	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	2	4	theme	cutaneous	296:304	arg1	form					342:345	the most common form	326:345	the most common form	326:345	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	8	5	theme	meglumine	1621:1629	arg1	antimoniate					1631:1641	meglumine antimoniate	1621:1641	meglumine antimoniate	1621:1641	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
32145429	4	6	theme	0.3	649:651	arg1	%					652:652	%	652:652	%	652:652	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	6	7	theme	cutaneous	1123:1131	arg1	studies					1143:1149	Ex vivo cutaneous retention studies	1115:1149	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells	1115:1194	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	7	8	theme	Topical	1272:1278	arg1	evaluation					1287:1296	Topical PCN-MA evaluation	1272:1296	Topical PCN-MA evaluation in murine leishmaniasis model	1272:1326	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	5	9	dep	%	904:904	arg1	to					899:900	to	899:900	to	899:900	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	6	10	theme	MA	1214:1215	arg1	retention					1227:1235	the MA cutaneous retention	1210:1235	the MA cutaneous retention	1210:1235	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	5	11	theme	regular	968:974	arg1	surface					976:982	the smooth and regular surface	953:982	surface	976:982	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	0	12	theme	in	151:152	arg1	nanocarriers					22:33	Sugar-based colloidal nanocarriers	0:33	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.	0:169	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	12	theme	in	151:152	arg1	evaluation					159:168	in vivo evaluation	151:168	in vivo evaluation	151:168	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	1	13	theme	protozoan	223:231	arg1	parasites					233:241	protozoan parasites	223:241	protozoan parasites from Leishmania species	223:265	The leishmaniases are a group of diseases caused by protozoan parasites from Leishmania species.					
32145429	1	14	from	species	259:265	arg1	parasites					233:241	protozoan parasites	223:241	protozoan parasites from Leishmania species	223:265	The leishmaniases are a group of diseases caused by protozoan parasites from Leishmania species.					
32145429	3	15	theme	topical	583:589	arg1	treatment					594:602	topical CL treatment	583:602	topical CL treatment	583:602	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	7	16	theme	area	1513:1516	arg1	deposition					1518:1527	collagen area deposition	1504:1527	collagen area deposition	1504:1527	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	6	17	theme	Franz	1174:1178	arg1	cells					1190:1194	Franz diffusion cells	1174:1194	Franz diffusion cells	1174:1194	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	5	18	theme	smooth	957:962	arg1	surface					976:982	the smooth and regular surface	953:982	surface	976:982	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	7	19	from	evaluation	1287:1296	arg1	model					1322:1326	murine leishmaniasis model	1301:1326	murine leishmaniasis model	1301:1326	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	0	20	dep	in	151:152	arg1	vivo					154:157	vivo	154:157	vivo	154:157	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	21	theme	leishmaniasis	94:106	arg1	treatment					108:116	cutaneous leishmaniasis treatment	84:116	cutaneous leishmaniasis treatment	84:116	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	7	22	theme	healing	1475:1481	arg1	activity					1483:1490	superior healing activity	1466:1490	superior healing activity	1466:1490	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	5	23	theme	nanosized	995:1003	arg1	scale					1005:1009	the nanosized scale	991:1009	the nanosized scale	991:1009	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	2	24	theme	available	391:399	arg1	antimoniate					425:435	meglumine antimoniate	415:435	meglumine antimoniate (MA)	415:440	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	2	24	theme	available	391:399	arg1	drugs					401:405	the available drugs	387:405	the available drugs such as meglumine antimoniate (MA)	387:440	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	5	25	theme	%	897:897	arg1	%					904:904	87% to 92%	895:904	87% to 92%	895:904	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	25	theme	%	897:897	arg1	efficiency					883:892	high encapsulation efficiency	864:892	high encapsulation efficiency (87% to 92%)	864:905	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	26	dep	microscopy	1053:1062	arg1	analysis					1105:1112	analysis	1105:1112	analysis	1105:1112	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	0	27	theme	Sugar-based	0:10	arg1	nanocarriers					22:33	Sugar-based colloidal nanocarriers	0:33	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.	0:169	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	27	theme	Sugar-based	0:10	arg1	retention					137:145	Ex vivo cutaneous retention	119:145	Ex vivo cutaneous retention	119:145	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	27	theme	Sugar-based	0:10	arg1	evaluation					159:168	in vivo evaluation	151:168	in vivo evaluation	151:168	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	2	28	theme	meglumine	415:423	arg1	MA					438:439	MA	438:439	MA	438:439	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	2	28	theme	meglumine	415:423	arg1	antimoniate					425:435	meglumine antimoniate	415:435	meglumine antimoniate (MA)	415:440	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	5	29	theme	dynamic	1074:1080	arg1	DLS					1100:1102	DLS	1100:1102	DLS	1100:1102	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	29	theme	dynamic	1074:1080	arg1	scattering					1088:1097	dynamic light scattering	1074:1097	dynamic light scattering (DLS)	1074:1103	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	7	30	theme	parasite	1437:1444	arg1	reduction					1452:1460	parasite titer reduction	1437:1460	parasite titer reduction	1437:1460	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	5	31	theme	encapsulation	869:881	arg1	%					904:904	87% to 92%	895:904	87% to 92%	895:904	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	31	theme	encapsulation	869:881	arg1	efficiency					883:892	high encapsulation efficiency	864:892	high encapsulation efficiency (87% to 92%)	864:905	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	0	32	theme	topical	39:45	arg1	application					69:79	topical meglumine antimoniate application	39:79	topical meglumine antimoniate application to cutaneous leishmaniasis treatment	39:116	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	5	33	theme	electron	1044:1051	arg1	SEM					1065:1067	SEM	1065:1067	SEM	1065:1067	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	33	theme	electron	1044:1051	arg1	microscopy					1053:1062	scanning electron microscopy	1035:1062	scanning electron microscopy (SEM)	1035:1068	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	34	theme	composite	911:919	arg1	particles					938:946	composite spherical-shaped particles	911:946	composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis	911:1112	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	2	35	theme	severe	450:455	arg1	reactions					465:473	severe adverse reactions	450:473	severe adverse reactions	450:473	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	0	36	theme	antimoniate	57:67	arg1	application					69:79	topical meglumine antimoniate application	39:79	topical meglumine antimoniate application to cutaneous leishmaniasis treatment	39:116	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	3	37	theme	colloidal	533:541	arg1	nanocarriers					543:554	maltodextrin polymeric colloidal nanocarriers	510:554	maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment	510:602	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	7	38	theme	reference	1394:1402	arg1	Glucantime®					1414:1424	Glucantime®	1414:1424	Glucantime®	1414:1424	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	7	38	theme	reference	1394:1402	arg1	medicine					1404:1411	the reference medicine	1390:1411	the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition	1390:1527	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	0	39	dep	nanocarriers	22:33	arg1	nanocarriers					22:33	Sugar-based colloidal nanocarriers	0:33	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.	0:169	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	39	dep	nanocarriers	22:33	arg1	retention					137:145	Ex vivo cutaneous retention	119:145	Ex vivo cutaneous retention	119:145	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	39	dep	nanocarriers	22:33	arg1	evaluation					159:168	in vivo evaluation	151:168	in vivo evaluation	151:168	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	4	40	theme	%	727:727	arg1	 Kolliphor® P-188					728:744	or no 3% Kolliphor® P-188	720:744	 Kolliphor® P-188	728:744	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	1	41	theme	Leishmania	248:257	arg1	species					259:265	Leishmania species	248:265	Leishmania species	248:265	The leishmaniases are a group of diseases caused by protozoan parasites from Leishmania species.					
32145429	4	42	dep	%	633:633	arg1	maltodextrin					635:646	maltodextrin	635:646	5 to 8% maltodextrin	627:646	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	7	43	theme	intraperitoneal	1361:1375	arg1	injection					1377:1385	the intraperitoneal injection	1357:1385	the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition	1357:1527	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	6	44	theme	ears	1161:1164	arg1	skin					1166:1169	pig ears skin	1157:1169	pig ears skin	1157:1169	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	4	45	theme	external	792:799	arg1	phase					801:805	a silicone-based external phase	775:805	a silicone-based external phase	775:805	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	3	46	theme	polymeric	523:531	arg1	nanocarriers					543:554	maltodextrin polymeric colloidal nanocarriers	510:554	maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment	510:602	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	8	47	theme	topical	1601:1607	arg1	delivery					1609:1616	topical delivery	1601:1616	topical delivery of meglumine antimoniate	1601:1641	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
32145429	7	48	theme	similar	1335:1341	arg1	efficacy					1343:1350	similar efficacy	1335:1350	similar efficacy	1335:1350	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	7	49	theme	leishmaniasis	1308:1320	arg1	model					1322:1326	murine leishmaniasis model	1301:1326	murine leishmaniasis model	1301:1326	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	6	50	theme	retention	1133:1141	arg1	studies					1143:1149	Ex vivo cutaneous retention studies	1115:1149	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells	1115:1194	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	5	51	with	silicone	850:857	arg1	%					904:904	87% to 92%	895:904	87% to 92%	895:904	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	51	with	silicone	850:857	arg1	efficiency					883:892	high encapsulation efficiency	864:892	high encapsulation efficiency (87% to 92%)	864:905	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	51	with	silicone	850:857	arg1	particles					938:946	composite spherical-shaped particles	911:946	composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis	911:1112	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	6	52	theme	cutaneous	1217:1225	arg1	retention					1227:1235	the MA cutaneous retention	1210:1235	the MA cutaneous retention	1210:1235	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	8	53	theme	antimoniate	1631:1641	arg1	delivery					1609:1616	topical delivery	1601:1616	topical delivery of meglumine antimoniate	1601:1641	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
32145429	1	54	theme	diseases	204:211	arg1	group					195:199	a group	193:199	a group of diseases caused by protozoan parasites from Leishmania species	193:265	The leishmaniases are a group of diseases caused by protozoan parasites from Leishmania species.					
32145429	1	54	theme	diseases	204:211	arg1	diseases					204:211	diseases	204:211	diseases caused by protozoan parasites from Leishmania species	204:265	The leishmaniases are a group of diseases caused by protozoan parasites from Leishmania species.					
32145429	1	54	theme	diseases	204:211	arg1	leishmaniases					175:187	The leishmaniases	171:187	The leishmaniases	171:187	The leishmaniases are a group of diseases caused by protozoan parasites from Leishmania species.					
32145429	7	55	theme	PCN-MA	1280:1285	arg1	evaluation					1287:1296	Topical PCN-MA evaluation	1272:1296	Topical PCN-MA evaluation in murine leishmaniasis model	1272:1326	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	3	56	theme	CL	591:592	arg1	treatment					594:602	topical CL treatment	583:602	topical CL treatment	583:602	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	7	57	theme	deposition	1518:1527	arg1	terms					1495:1499	terms	1495:1499	terms of collagen area deposition	1495:1527	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	6	58	theme	Ex	1115:1116	arg1	studies					1143:1149	Ex vivo cutaneous retention studies	1115:1149	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells	1115:1194	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	5	59	with	efficiency	883:892	arg1	surface					976:982	the smooth and regular surface	953:982	surface	976:982	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	4	60	contain	containing	709:718	arg2	 Kolliphor® P-188					728:744	or no 3% Kolliphor® P-188	720:744	 Kolliphor® P-188	728:744	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	4	60	contain	containing	709:718	arg1	%					661:661	1%	660:661	1% MA in 21% of water as aqueous-internal phase	660:706	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	5	61	theme	colloidal	820:828	arg1	system					830:835	a colloidal system	818:835	a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis	818:1112	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	7	62	theme	collagen	1504:1511	arg1	deposition					1518:1527	collagen area deposition	1504:1527	collagen area deposition	1504:1527	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	6	63	theme	diffusion	1180:1188	arg1	cells					1190:1194	Franz diffusion cells	1174:1194	Franz diffusion cells	1174:1194	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	0	64	theme	cutaneous	84:92	arg1	treatment					108:116	cutaneous leishmaniasis treatment	84:116	cutaneous leishmaniasis treatment	84:116	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	2	65	theme	Effectiveness	268:280	arg1	therapies					282:290	Effectiveness therapies	268:290	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form,	268:346	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	4	66	theme	%	652:652	arg1	NaCl					654:657	0.3% NaCl	649:657	0.3% NaCl	649:657	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	7	67	theme	superior	1466:1473	arg1	activity					1483:1490	superior healing activity	1466:1490	superior healing activity	1466:1490	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	6	68	dep	Ex	1115:1116	arg1	vivo					1118:1121	vivo	1118:1121	vivo	1118:1121	Ex vivo cutaneous retention studies using pig ears skin on Franz diffusion cells revealed that the MA cutaneous retention is improved when delivered by PCN.					
32145429	0	69	theme	Ex	119:120	arg1	nanocarriers					22:33	Sugar-based colloidal nanocarriers	0:33	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.	0:169	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	69	theme	Ex	119:120	arg1	retention					137:145	Ex vivo cutaneous retention	119:145	Ex vivo cutaneous retention	119:145	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	5	70	theme	scanning	1035:1042	arg1	SEM					1065:1067	SEM	1065:1067	SEM	1065:1067	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	70	theme	scanning	1035:1042	arg1	microscopy					1053:1062	scanning electron microscopy	1035:1062	scanning electron microscopy (SEM)	1035:1068	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	4	71	theme	aqueous-internal	685:700	arg1	phase					702:706	aqueous-internal phase	685:706	aqueous-internal phase	685:706	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	0	72	theme	cutaneous	127:135	arg1	nanocarriers					22:33	Sugar-based colloidal nanocarriers	0:33	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.	0:169	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	72	theme	cutaneous	127:135	arg1	retention					137:145	Ex vivo cutaneous retention	119:145	Ex vivo cutaneous retention	119:145	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	73	dep	Ex	119:120	arg1	vivo					122:125	vivo	122:125	vivo	122:125	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	74	theme	colloidal	12:20	arg1	nanocarriers					22:33	Sugar-based colloidal nanocarriers	0:33	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.	0:169	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	74	theme	colloidal	12:20	arg1	retention					137:145	Ex vivo cutaneous retention	119:145	Ex vivo cutaneous retention	119:145	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	0	74	theme	colloidal	12:20	arg1	evaluation					159:168	in vivo evaluation	151:168	in vivo evaluation	151:168	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	5	75	theme	high	864:867	arg1	%					904:904	87% to 92%	895:904	87% to 92%	895:904	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	75	theme	high	864:867	arg1	efficiency					883:892	high encapsulation efficiency	864:892	high encapsulation efficiency (87% to 92%)	864:905	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	4	76	theme	water	676:680	arg1	water					676:680	water	676:680	water	676:680	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	4	76	theme	water	676:680	arg1	%					671:671	21%	669:671	21% of water	669:680	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	0	77	theme	meglumine	47:55	arg1	antimoniate					57:67	meglumine antimoniate	47:67	topical meglumine antimoniate application to cutaneous leishmaniasis treatment	39:116	Sugar-based colloidal nanocarriers for topical meglumine antimoniate application to cutaneous leishmaniasis treatment: Ex vivo cutaneous retention and in vivo evaluation.					
32145429	5	78	theme	light	1082:1086	arg1	DLS					1100:1102	DLS	1100:1102	DLS	1100:1102	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	5	78	theme	light	1082:1086	arg1	scattering					1088:1097	dynamic light scattering	1074:1097	dynamic light scattering (DLS)	1074:1103	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	7	79	theme	titer	1446:1450	arg1	reduction					1452:1460	parasite titer reduction	1437:1460	parasite titer reduction	1437:1460	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	4	80	dep	8	632:632	arg1	to					629:630	to	629:630	to	629:630	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	2	81	theme	adverse	457:463	arg1	reactions					465:473	severe adverse reactions	450:473	severe adverse reactions	450:473	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	5	82	theme	spherical-shaped	921:936	arg1	particles					938:946	composite spherical-shaped particles	911:946	composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis	911:1112	It formed a colloidal system dispersed in silicone with high encapsulation efficiency (87% to 92%) and composite spherical-shaped particles with the smooth and regular surface within the nanosized scale, which was confirmed by scanning electron microscopy (SEM) and dynamic light scattering (DLS) analysis.					
32145429	3	83	contain	containing	556:565	arg1	nanocarriers					543:554	maltodextrin polymeric colloidal nanocarriers	510:554	maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment	510:602	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	3	83	contain	containing	556:565	arg2	PCN-MA					571:576	PCN-MA	571:576	PCN-MA	571:576	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	3	83	contain	containing	556:565	arg2	MA					567:568	MA	567:568	MA (PCN-MA)	567:577	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	8	84	theme	sugar-based	1560:1570	arg1	PCN					1572:1574	this sugar-based PCN	1555:1574	this sugar-based PCN	1555:1574	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
32145429	8	84	theme	sugar-based	1560:1570	arg1	agent					1591:1595	a promising agent	1579:1595	a promising agent for topical delivery of meglumine antimoniate	1579:1641	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
32145429	2	85	theme	common	335:340	arg1	form					342:345	the most common form	326:345	the most common form	326:345	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	2	85	theme	common	335:340	arg1	leishmaniasis					306:318	cutaneous leishmaniasis	296:318	cutaneous leishmaniasis (CL)	296:323	Effectiveness therapies for cutaneous leishmaniasis (CL), the most common form, are still needed to be developed since the available drugs such as meglumine antimoniate (MA) present severe adverse reactions.					
32145429	7	86	theme	medicine	1404:1411	arg1	injection					1377:1385	the intraperitoneal injection	1357:1385	the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition	1357:1527	Topical PCN-MA evaluation in murine leishmaniasis model showed similar efficacy than the intraperitoneal injection of the reference medicine (Glucantime®) regarding parasite titer reduction and superior healing activity in terms of collagen area deposition.					
32145429	4	87	theme	3	726:726	arg1	%					727:727	%	727:727	%	727:727	PCN-MA is composed of 5 to 8% maltodextrin, 0.3% NaCl, 1% MA in 21% of water as aqueous-internal phase, containing or no 3% Kolliphor® P-188, and 10% SF1540 dispersed in a silicone-based external phase.					
32145429	3	88	theme	maltodextrin	510:521	arg1	nanocarriers					543:554	maltodextrin polymeric colloidal nanocarriers	510:554	maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment	510:602	Here, we develop and characterize maltodextrin polymeric colloidal nanocarriers containing MA (PCN-MA) for topical CL treatment.					
32145429	8	89	theme	promising	1581:1589	arg1	PCN					1572:1574	this sugar-based PCN	1555:1574	this sugar-based PCN	1555:1574	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
32145429	8	89	theme	promising	1581:1589	arg1	agent					1591:1595	a promising agent	1579:1595	a promising agent for topical delivery of meglumine antimoniate	1579:1641	Our results suggest that this sugar-based PCN is a promising agent for topical delivery of meglumine antimoniate.					
34416923	12	0	dep	potential	2572:2580	arg1	therapeutic					2582:2592	therapeutic	2582:2592	therapeutic	2582:2592	The TNF-α-TSG-6-HC-HA pathway may represent a potential therapeutic target in OA.					
34416923	8	1	located	localized	1865:1873	arg1	joints					1941:1946	OA joints	1938:1946	OA joints	1938:1946	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	8	1	located	localized	1865:1873	arg2	HC-HA					1855:1859	HC-HA	1855:1859	HC-HA	1855:1859	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	11	2	theme	macromolecular	2297:2310	arg1	aggregation					2312:2322	macromolecular aggregation	2297:2322	macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions	2297:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	10	3	theme	TSG6	2179:2182	arg1	expression					2189:2198	cartilage TSG6 gene expression	2169:2198	cartilage TSG6 gene expression	2169:2198	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	5	4	theme	nanopore	1291:1298	arg1	measurements					1300:1311	solid-state nanopore measurements	1279:1311	solid-state nanopore measurements	1279:1311	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	8	5	theme	TSG-6-mediated	1752:1765	arg1	formation					1781:1789	TSG-6-mediated HC-HA complex formation	1752:1789	TSG-6-mediated HC-HA complex formation	1752:1789	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	2	6	theme	HC-HA	568:572	arg1	formation					582:590	TSG-6-mediated HC-HA complex formation	553:590	TSG-6-mediated HC-HA complex formation	553:590	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	7	7	theme	synovial	1719:1726	arg1	membrane					1728:1735	OA synovial membrane	1716:1735	OA synovial membrane	1716:1735	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	3	8	theme	TNF-α-TSG-6-HC-HA	669:685	arg1	pathway					697:703	the TNF-α-TSG-6-HC-HA signaling pathway	665:703	the TNF-α-TSG-6-HC-HA signaling pathway	665:703	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	10	9	theme	fluid	2119:2123	arg1	concentrations					2131:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	6	10	theme	TSG-6-mediated	1428:1441	arg1	crosslinking					1446:1457	TSG-6-mediated HA crosslinking	1428:1457	TSG-6-mediated HA crosslinking	1428:1457	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	1	11	theme	polysaccharide	279:292	arg1	HA					311:312	HA	311:312	HA	311:312	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	11	theme	polysaccharide	279:292	arg1	acid					305:308	the polysaccharide hyaluronic acid	275:308	the polysaccharide hyaluronic acid (HA)	275:313	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	2	12	theme	OA	605:606	arg1	model					608:612	an equine OA model	595:612	an equine OA model	595:612	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	3	13	from	impact	818:823	arg1	composition					849:859	synovial fluid composition	834:859	synovial fluid composition	834:859	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	3	13	from	impact	818:823	arg1	properties					877:886	biophysical properties	865:886	biophysical properties	865:886	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	8	14	theme	synovial	1809:1816	arg1	fluid					1818:1822	OA synovial fluid	1806:1822	OA synovial fluid	1806:1822	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	5	15	theme	gel	1255:1257	arg1	electrophoresis					1259:1273	agarose gel electrophoresis	1247:1273	agarose gel electrophoresis	1247:1273	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	8	16	theme	OA	1938:1939	arg1	joints					1941:1946	OA joints	1938:1946	OA joints	1938:1946	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	10	17	theme	equine	2247:2252	arg1	OA					2254:2255	equine OA	2247:2255	equine OA	2247:2255	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	6	18	used	used	1411:1414	arg2	SEC-MALS					1398:1405	SEC-MALS	1398:1405	SEC-MALS	1398:1405	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	3	19	theme	multiple	712:719	arg1	tissues					727:733	multiple joint tissues	712:733	multiple joint tissues	712:733	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	4	20	from	OA	1016:1017	arg1	fluid					1002:1006	synovial fluid	993:1006	synovial fluid from 63 OA and 25 control joints	993:1039	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	7	21	theme	TSG6	1681:1684	arg1	expression					1686:1695	TSG6 expression	1681:1695	TSG6 expression	1681:1695	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	4	22	theme	synovial	993:1000	arg1	fluid					1002:1006	synovial fluid	993:1006	synovial fluid from 63 OA and 25 control joints	993:1039	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	11	23	theme	fluid	2469:2473	arg1	viscosity					2475:2483	synovial fluid viscosity	2460:2483	synovial fluid viscosity	2460:2483	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	1	24	theme	pathological	391:402	arg1	conditions					404:413	pathological conditions	391:413	pathological conditions such as osteoarthritis (OA)	391:441	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	24	theme	pathological	391:402	arg1	osteoarthritis					423:436	osteoarthritis	423:436	osteoarthritis (OA)	423:441	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	6	25	theme	HA	1483:1484	arg1	viscosities					1495:1505	synovial fluid and HA solution viscosities	1464:1505	synovial fluid and HA solution viscosities	1464:1505	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	4	26	theme	synthase	1049:1056	arg1	expression					1126:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	27	dep	concentrations	926:939	arg1	IL-1β					949:953	IL-1β	949:953	IL-1β	949:953	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	27	dep	concentrations	926:939	arg1	TNF-α					942:946	TNF-α	942:946	TNF-α	942:946	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	27	dep	concentrations	926:939	arg1	CCL2					956:959	CCL2	956:959	CCL2	956:959	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	11	28	theme	low	2327:2329	arg1	HA					2334:2335	low MW HA	2327:2335	low MW HA with resultant increases in the viscosity of low MW HA solutions	2327:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	6	29	theme	fluid	1473:1477	arg1	viscosities					1495:1505	synovial fluid and HA solution viscosities	1464:1505	synovial fluid and HA solution viscosities	1464:1505	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	1	30	theme	TNF-α-stimulated	132:147	arg1	gene					149:152	BACKGROUND TNF-α-stimulated gene 6	121:154	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	30	theme	TNF-α-stimulated	132:147	arg1	TSG-6					157:161	TSG-6	157:161	TSG-6	157:161	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	8	31	theme	complex	1773:1779	arg1	formation					1781:1789	TSG-6-mediated HC-HA complex formation	1752:1789	TSG-6-mediated HC-HA complex formation	1752:1789	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	11	32	theme	HA	2497:2498	arg1	MW					2500:2501	HA MW	2497:2501	HA MW	2497:2501	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	10	33	theme	CONCLUSIONS	2098:2108	arg1	concentrations					2131:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	7	34	theme	OA	1658:1659	arg1	fluid					1670:1674	OA synovial fluid	1658:1674	OA synovial fluid	1658:1674	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	12	35	from	target	2594:2599	arg1	OA					2604:2605	OA	2604:2605	OA	2604:2605	The TNF-α-TSG-6-HC-HA pathway may represent a potential therapeutic target in OA.					
34416923	2	36	theme	gene	491:494	arg1	expression					496:505	HA synthase and inflammatory gene expression	462:505	expression	496:505	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	10	37	theme	synovial	2147:2154	arg1	membrane					2156:2163	synovial membrane	2147:2163	synovial membrane	2147:2163	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	0	38	from	synthesis	16:24	arg1	osteoarthritis					67:80	equine osteoarthritis	60:80	equine osteoarthritis	60:80	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	11	39	theme	MW	2386:2387	arg1	solutions					2392:2400	low MW HA solutions	2382:2400	low MW HA solutions	2382:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	1	40	theme	heavy	342:346	arg1	complexes					378:386	heavy chain-hyaluronic acid (HC-HA) complexes	342:386	heavy chain-hyaluronic acid (HC-HA) complexes	342:386	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	11	41	theme	viscosity	2475:2483	arg1	determinant					2445:2455	the primary determinant	2433:2455	the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking	2433:2523	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	11	41	theme	viscosity	2475:2483	arg1	concentration					2415:2427	HA concentration	2412:2427	HA concentration	2412:2427	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	6	42	theme	microrheology	1554:1566	arg1	measurements					1585:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	10	43	theme	complex	2211:2217	arg1	formation					2219:2227	HC-HA complex formation	2205:2227	HC-HA complex formation	2205:2227	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	2	44	theme	fluid	517:521	arg1	HA					523:524	synovial fluid HA	508:524	synovial fluid HA	508:524	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	4	45	theme	hyaluronan-degrading	1079:1098	arg1	enzyme					1100:1105	hyaluronan-degrading enzyme	1079:1105	hyaluronan-degrading enzyme	1079:1105	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	0	46	theme	TNF-α-TSG-6-mediated	83:102	arg1	formation					110:118	TNF-α-TSG-6-mediated HC-HA formation	83:118	TNF-α-TSG-6-mediated HC-HA formation	83:118	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	1	47	theme	acid	365:368	arg1	complexes					378:386	heavy chain-hyaluronic acid (HC-HA) complexes	342:386	heavy chain-hyaluronic acid (HC-HA) complexes	342:386	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	6	48	theme	multiple	1527:1534	arg1	measurements					1585:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	0	49	dep	formation	110:118	arg1	synthesis					16:24	Hyaluronic acid synthesis	0:24	Hyaluronic acid synthesis	0:24	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	0	49	dep	formation	110:118	arg1	degradation					27:37	degradation	27:37	degradation	27:37	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	0	49	dep	formation	110:118	arg1	crosslinking					44:55	crosslinking	44:55	crosslinking	44:55	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	3	50	theme	synovial	834:841	arg1	composition					849:859	synovial fluid composition	834:859	synovial fluid composition	834:859	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	8	51	theme	synovial	1883:1890	arg1	membrane					1892:1899	synovial membrane	1883:1899	synovial membrane	1883:1899	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	5	52	theme	HC-HA	1318:1322	arg1	formation					1332:1340	HC-HA complex formation	1318:1340	HC-HA complex formation	1318:1340	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	12	53	theme	TNF-α-TSG-6-HC-HA	2530:2546	arg1	pathway					2548:2554	The TNF-α-TSG-6-HC-HA pathway	2526:2554	The TNF-α-TSG-6-HC-HA pathway	2526:2554	The TNF-α-TSG-6-HC-HA pathway may represent a potential therapeutic target in OA.					
34416923	5	54	theme	HA	1186:1187	arg1	MW					1207:1208	MW	1207:1208	MW	1207:1208	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	5	54	theme	HA	1186:1187	arg1	weight					1199:1204	HA molecular weight	1186:1204	HA molecular weight (MW) distributions	1186:1223	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	3	55	dep	determine	804:812	arg1	2					801:801	2	801:801	2	801:801	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	3	56	theme	OA	828:829	arg1	impact					818:823	the impact	814:823	the impact of OA on synovial fluid composition and biophysical properties	814:886	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	0	57	from	degradation	27:37	arg1	osteoarthritis					67:80	equine osteoarthritis	60:80	equine osteoarthritis	60:80	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	4	58	theme	cytokine	917:924	arg1	concentrations					926:939	inflammatory cytokine concentrations	904:939	inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11)	904:974	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	59	theme	synovial	1153:1160	arg1	membrane					1162:1169	synovial membrane	1153:1169	synovial membrane	1153:1169	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	0	60	theme	Hyaluronic	0:9	arg1	synthesis					16:24	Hyaluronic acid synthesis	0:24	Hyaluronic acid synthesis	0:24	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	1	61	theme	hyaluronic	294:303	arg1	HA					311:312	HA	311:312	HA	311:312	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	61	theme	hyaluronic	294:303	arg1	acid					305:308	the polysaccharide hyaluronic acid	275:308	the polysaccharide hyaluronic acid (HA)	275:313	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	3	62	theme	biophysical	865:875	arg1	properties					877:886	biophysical properties	865:886	biophysical properties	865:886	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	11	63	theme	solutions	2392:2400	arg1	viscosity					2369:2377	the viscosity	2365:2377	the viscosity of low MW HA solutions	2365:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	2	64	theme	HA	462:463	arg1	synthase					465:472	HA synthase and inflammatory gene expression	462:505	synthase	465:472	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	11	65	theme	resultant	2342:2350	arg1	increases					2352:2360	resultant increases	2342:2360	resultant increases in the viscosity of low MW HA solutions	2342:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	9	66	theme	MW	2005:2006	arg1	HA					2008:2009	low MW HA	2001:2009	low MW HA	2001:2009	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	3	67	dep	evaluate	656:663	arg1	1					653:653	1	653:653	1	653:653	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	2	68	theme	inflammatory	478:489	arg1	expression					496:505	HA synthase and inflammatory gene expression	462:505	expression	496:505	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	8	69	dep	membrane	1892:1899	arg1	chondrocytes					1922:1933	chondrocytes	1922:1933	chondrocytes	1922:1933	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	5	70	theme	agarose	1247:1253	arg1	electrophoresis					1259:1273	agarose gel electrophoresis	1247:1273	agarose gel electrophoresis	1247:1273	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	11	71	theme	low	2382:2384	arg1	solutions					2392:2400	low MW HA solutions	2382:2400	low MW HA solutions	2382:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	9	72	theme	TSG-6	2030:2034	arg1	presence					2018:2025	the presence	2014:2025	the presence of TSG-6 and inter-α-inhibitor	2014:2056	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	11	73	theme	MW	2331:2332	arg1	HA					2334:2335	low MW HA	2327:2335	low MW HA with resultant increases in the viscosity of low MW HA solutions	2327:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	4	74	from	joints	1034:1039	arg1	fluid					1002:1006	synovial fluid	993:1006	synovial fluid from 63 OA and 25 control joints	993:1039	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	9	75	theme	inter-α-inhibitor	2040:2056	arg1	presence					2018:2025	the presence	2014:2025	the presence of TSG-6 and inter-α-inhibitor	2014:2056	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	11	76	theme	HA	2412:2413	arg1	determinant					2445:2455	the primary determinant	2433:2455	the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking	2433:2523	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	11	76	theme	HA	2412:2413	arg1	concentration					2415:2427	HA concentration	2412:2427	HA concentration	2412:2427	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	2	77	theme	complex	574:580	arg1	formation					582:590	TSG-6-mediated HC-HA complex formation	553:590	TSG-6-mediated HC-HA complex formation	553:590	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	7	78	theme	OA	1716:1717	arg1	membrane					1728:1735	OA synovial membrane	1716:1735	OA synovial membrane	1716:1735	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	11	79	theme	TSG-6	2281:2285	arg1	ability					2270:2276	the ability	2266:2276	the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro	2266:2409	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	9	80	theme	concurrent	2063:2072	arg1	increases					2074:2082	concurrent increases	2063:2082	concurrent increases in viscosity	2063:2095	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	8	81	theme	OA	1806:1807	arg1	fluid					1818:1822	OA synovial fluid	1806:1822	OA synovial fluid	1806:1822	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	2	82	theme	TSG-6-mediated	553:566	arg1	formation					582:590	TSG-6-mediated HC-HA complex formation	553:590	TSG-6-mediated HC-HA complex formation	553:590	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	3	83	theme	signaling	687:695	arg1	pathway					697:703	the TNF-α-TSG-6-HC-HA signaling pathway	665:703	the TNF-α-TSG-6-HC-HA signaling pathway	665:703	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	2	84	theme	equine	598:603	arg1	model					608:612	an equine OA model	595:612	an equine OA model	595:612	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	4	85	theme	control	1026:1032	arg1	joints					1034:1039	63 OA and 25 control joints	1013:1039	joints	1034:1039	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	1	86	theme	TNF-α-responsive	175:190	arg1	hyaladherin					192:202	a TNF-α-responsive hyaladherin	173:202	a TNF-α-responsive hyaladherin	173:202	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	86	theme	TNF-α-responsive	175:190	arg1	protein					164:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	11	87	with	HA	2334:2335	arg1	increases					2352:2360	resultant increases	2342:2360	resultant increases in the viscosity of low MW HA solutions	2342:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	3	88	theme	joint	721:725	arg1	tissues					727:733	multiple joint tissues	712:733	multiple joint tissues	712:733	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	5	89	theme	solid-state	1279:1289	arg1	measurements					1300:1311	solid-state nanopore measurements	1279:1311	solid-state nanopore measurements	1279:1311	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	10	90	theme	gene	2184:2187	arg1	expression					2189:2198	cartilage TSG6 gene expression	2169:2198	cartilage TSG6 gene expression	2169:2198	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	11	91	theme	synovial	2460:2467	arg1	fluid					2469:2473	synovial fluid	2460:2473	synovial fluid viscosity	2460:2483	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	4	92	dep	METHODS	889:895	arg1	HA					897:898	HA	897:898	HA	897:898	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	92	dep	METHODS	889:895	arg1	concentrations					926:939	inflammatory cytokine concentrations	904:939	inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11)	904:974	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	1	93	theme	enzymatic	215:223	arg1	activity					225:232	enzymatic activity	215:232	enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA)	215:441	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	10	94	theme	TNF-α	2125:2129	arg1	concentrations					2131:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	3	95	theme	synovial	746:753	arg1	membrane					755:762	synovial membrane	746:762	synovial membrane	746:762	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	1	96	theme	BACKGROUND	121:130	arg1	gene					149:152	BACKGROUND TNF-α-stimulated gene 6	121:154	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	96	theme	BACKGROUND	121:130	arg1	TSG-6					157:161	TSG-6	157:161	TSG-6	157:161	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	10	97	theme	Synovial	2110:2117	arg1	concentrations					2131:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations	2098:2144	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	6	98	theme	synovial	1464:1471	arg1	fluid					1473:1477	synovial fluid	1464:1477	synovial fluid	1464:1477	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	1	99	theme	gene	149:152	arg1	hyaladherin					192:202	a TNF-α-responsive hyaladherin	173:202	a TNF-α-responsive hyaladherin	173:202	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	99	theme	gene	149:152	arg1	protein					164:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	6	100	theme	microfluidic	1572:1583	arg1	measurements					1585:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	11	101	theme	HA	2389:2390	arg1	solutions					2392:2400	low MW HA solutions	2382:2400	low MW HA solutions	2382:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	0	102	theme	equine	60:65	arg1	osteoarthritis					67:80	equine osteoarthritis	60:80	equine osteoarthritis	60:80	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	7	103	theme	synovial	1661:1668	arg1	fluid					1670:1674	OA synovial fluid	1658:1674	OA synovial fluid	1658:1674	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	4	104	theme	inflammatory	904:915	arg1	concentrations					926:939	inflammatory cytokine concentrations	904:939	inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11)	904:974	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	105	theme	HA	1046:1047	arg1	HAS1-3					1059:1064	HAS1-3	1059:1064	HAS1-3	1059:1064	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	105	theme	HA	1046:1047	arg1	synthase					1049:1056	HA synthase	1046:1056	HA synthase (HAS1-3)	1046:1065	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	4	105	theme	HA	1046:1047	arg1	HYAL2					1108:1112	HYAL2	1108:1112	HYAL2	1108:1112	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	8	106	theme	superficial	1905:1915	arg1	zone					1917:1920	superficial zone	1905:1920	superficial zone	1905:1920	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	6	107	theme	HA	1443:1444	arg1	crosslinking					1446:1457	TSG-6-mediated HA crosslinking	1428:1457	TSG-6-mediated HA crosslinking	1428:1457	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	1	108	contain	possesses	205:213	arg2	activity					225:232	enzymatic activity	215:232	enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA)	215:441	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	108	contain	possesses	205:213	arg1	hyaladherin					192:202	a TNF-α-responsive hyaladherin	173:202	a TNF-α-responsive hyaladherin	173:202	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	108	contain	possesses	205:213	arg1	protein					164:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein	121:170	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	4	109	theme	enzyme	1100:1105	arg1	expression					1126:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	0	110	theme	HC-HA	104:108	arg1	formation					110:118	TNF-α-TSG-6-mediated HC-HA formation	83:118	TNF-α-TSG-6-mediated HC-HA formation	83:118	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	1	111	theme	chain-hyaluronic	348:363	arg1	HC-HA					371:375	HC-HA	371:375	HC-HA	371:375	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	1	111	theme	chain-hyaluronic	348:363	arg1	acid					365:368	chain-hyaluronic acid	348:368	heavy chain-hyaluronic acid (HC-HA) complexes	342:386	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	6	112	theme	particle-tracking	1536:1552	arg1	measurements					1585:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	multiple particle-tracking microrheology and microfluidic measurements	1527:1596	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	2	113	theme	synovial	508:515	arg1	HA					523:524	synovial fluid HA	508:524	synovial fluid HA	508:524	Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model.					
34416923	0	114	from	crosslinking	44:55	arg1	osteoarthritis					67:80	equine osteoarthritis	60:80	equine osteoarthritis	60:80	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	10	115	theme	cartilage	2169:2177	arg1	expression					2189:2198	cartilage TSG6 gene expression	2169:2198	cartilage TSG6 gene expression	2169:2198	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	3	116	theme	synovial	780:787	arg1	fluid					789:793	synovial fluid	780:793	synovial fluid	780:793	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	7	117	theme	RESULTS	1613:1619	arg1	concentrations					1627:1640	RESULTS TNF-α concentrations	1613:1640	RESULTS TNF-α concentrations	1613:1640	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	3	118	theme	fluid	843:847	arg1	composition					849:859	synovial fluid composition	834:859	synovial fluid composition	834:859	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	5	119	theme	complex	1324:1330	arg1	formation					1332:1340	HC-HA complex formation	1318:1340	HC-HA complex formation	1318:1340	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	4	120	theme	TSG-6	1068:1072	arg1	expression					1126:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	5	121	theme	molecular	1189:1197	arg1	MW					1207:1208	MW	1207:1208	MW	1207:1208	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	5	121	theme	molecular	1189:1197	arg1	weight					1199:1204	HA molecular weight	1186:1204	HA molecular weight (MW) distributions	1186:1223	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	0	122	theme	acid	11:14	arg1	synthesis					16:24	Hyaluronic acid synthesis	0:24	Hyaluronic acid synthesis	0:24	Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation.					
34416923	5	123	theme	weight	1199:1204	arg1	distributions					1211:1223	HA molecular weight (MW) distributions	1186:1223	HA molecular weight (MW) distributions	1186:1223	HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence.					
34416923	11	124	theme	primary	2437:2443	arg1	determinant					2445:2455	the primary determinant	2433:2455	the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking	2433:2523	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	11	124	theme	primary	2437:2443	arg1	concentration					2415:2427	HA concentration	2412:2427	HA concentration	2412:2427	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	11	125	theme	HA	2334:2335	arg1	aggregation					2312:2322	macromolecular aggregation	2297:2322	macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions	2297:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	12	126	theme	potential	2572:2580	arg1	target					2594:2599	a potential therapeutic target	2570:2599	a potential therapeutic target in OA	2570:2605	The TNF-α-TSG-6-HC-HA pathway may represent a potential therapeutic target in OA.					
34416923	9	127	from	increases	2074:2082	arg1	viscosity					2087:2095	viscosity	2087:2095	viscosity	2087:2095	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	1	128	theme	acid	305:308	arg1	crosslinks					261:270	covalent crosslinks	252:270	covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA)	252:441	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
34416923	6	129	theme	solution	1486:1493	arg1	viscosities					1495:1505	synovial fluid and HA solution viscosities	1464:1505	synovial fluid and HA solution viscosities	1464:1505	SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively.					
34416923	9	130	theme	low	2001:2003	arg1	HA					2008:2009	low MW HA	2001:2009	low MW HA	2001:2009	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	7	131	theme	TNF-α	1621:1625	arg1	concentrations					1627:1640	RESULTS TNF-α concentrations	1613:1640	RESULTS TNF-α concentrations	1613:1640	RESULTS TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage.					
34416923	9	132	theme	HA	2008:2009	arg1	aggregation					1986:1996	macromolecular aggregation	1971:1996	macromolecular aggregation of low MW HA	1971:2009	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	9	133	theme	macromolecular	1971:1984	arg1	aggregation					1986:1996	macromolecular aggregation	1971:1996	macromolecular aggregation of low MW HA	1971:2009	SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity.					
34416923	10	134	theme	HC-HA	2205:2209	arg1	formation					2219:2227	HC-HA complex formation	2205:2227	HC-HA complex formation	2205:2227	CONCLUSIONS Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA.					
34416923	3	135	theme	study	638:642	arg1	objectives					619:628	The objectives	615:628	The objectives of this study	615:642	The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties.					
34416923	8	136	theme	HC-HA	1767:1771	arg1	formation					1781:1789	TSG-6-mediated HC-HA complex formation	1752:1789	TSG-6-mediated HC-HA complex formation	1752:1789	TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints.					
34416923	11	137	theme	HC-HA	2506:2510	arg1	crosslinking					2512:2523	HC-HA crosslinking	2506:2523	HC-HA crosslinking	2506:2523	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	4	138	theme	gene	1121:1124	arg1	expression					1126:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression	1046:1135	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	11	139	from	increases	2352:2360	arg1	viscosity					2369:2377	the viscosity	2365:2377	the viscosity of low MW HA solutions	2365:2400	Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking.					
34416923	4	140	dep	HYAL2	1108:1112	arg1	HEXA					1115:1118	HEXA	1115:1118	HEXA	1115:1118	METHODS HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage.					
34416923	1	141	theme	covalent	252:259	arg1	crosslinks					261:270	covalent crosslinks	252:270	covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA)	252:441	BACKGROUND TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA).					
32475571	0	0	theme	shells	77:82	arg1	studies					62:68	the cellulose composition studies	36:68	the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials	36:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	5	1	theme	thermogravimetric	649:665	arg1	analysis					667:674	thermogravimetric analysis	649:674	thermogravimetric analysis	649:674	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	7	2	theme	tensile	850:856	arg1	performances					858:869	The tensile performances	846:869	The tensile performances on the prepared cardboard	846:895	The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material.					
32475571	5	3	theme	purification	596:607	arg1	step					609:612	each purification step	591:612	each purification step	591:612	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	5	4	from	step	609:612	arg1	composition					548:558	the relative composition	535:558	the relative composition of lignin and holocellulose at each purification step	535:612	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	7	5	theme	prepared	878:885	arg1	cardboard					887:895	the prepared cardboard	874:895	the prepared cardboard	874:895	The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material.					
32475571	0	6	theme	Prunus	87:92	arg1	dulcis					94:99	Prunus dulcis	87:99	Prunus dulcis	87:99	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	8	7	theme	almond	1054:1059	arg1	firewood					1092:1099	firewood	1092:1099	firewood	1092:1099	These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.					
32475571	8	7	theme	almond	1054:1059	arg1	shells					1061:1066	the almond shells	1050:1066	the almond shells that are mainly used as firewood	1050:1099	These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.					
32475571	6	8	theme	circular	740:747	arg1	framework					757:765	a circular economy framework	738:765	a circular economy framework	738:765	To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard.					
32475571	4	9	theme	CPMAS	467:471	arg1	spectroscopy					477:488	13C CPMAS NMR spectroscopy	463:488	13C CPMAS NMR spectroscopy methodology	463:500	The materials before and after purification have been characterized by 13C CPMAS NMR spectroscopy methodology.					
32475571	2	10	theme	dulcis	286:291	arg1	Webb					307:310	the Prunus dulcis Miller (D.A.) Webb	275:310	the Prunus dulcis Miller (D.A.) Webb	275:310	In particular, the Prunus dulcis Miller (D.A.) Webb.					
32475571	5	11	theme	proper	505:510	arg1	analysis					517:524	A proper data analysis	503:524	A proper data analysis	503:524	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	3	12	from	available	363:371	arg1	Italy					385:389	southern Italy	376:389	southern Italy	376:389	was considered as a agro industrial waste largely available in southern Italy.					
32475571	8	13	theme	obtained	998:1005	arg1	microcellulose					1007:1020	the obtained microcellulose	994:1020	the obtained microcellulose	994:1020	These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.					
32475571	2	14	theme	Prunus	279:284	arg1	Webb					307:310	the Prunus dulcis Miller (D.A.) Webb	275:310	the Prunus dulcis Miller (D.A.) Webb	275:310	In particular, the Prunus dulcis Miller (D.A.) Webb.					
32475571	3	15	theme	agro	333:336	arg1	waste					349:353	a agro industrial waste	331:353	a agro industrial waste largely available in southern Italy	331:389	was considered as a agro industrial waste largely available in southern Italy.					
32475571	4	16	theme	spectroscopy	477:488	arg1	methodology					490:500	13C CPMAS NMR spectroscopy methodology	463:500	13C CPMAS NMR spectroscopy methodology	463:500	The materials before and after purification have been characterized by 13C CPMAS NMR spectroscopy methodology.					
32475571	7	17	theme	sustainable	948:958	arg1	material					960:967	a sustainable material	946:967	a sustainable material	946:967	The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material.					
32475571	7	17	theme	sustainable	948:958	arg1	suitability					908:918	its suitability	904:918	its suitability for packaging purposes	904:941	The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material.					
32475571	3	18	theme	southern	376:383	arg1	Italy					385:389	southern Italy	376:389	southern Italy	376:389	was considered as a agro industrial waste largely available in southern Italy.					
32475571	0	19	link	derived	111:117	arg1	materials					130:138	their derived cellulosic materials	105:138	their derived cellulosic materials	105:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	0	20	theme	state	6:10	arg1	13C-NMR					12:18	Solid state 13C-NMR	0:18	Solid state 13C-NMR	0:18	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	4	21	theme	NMR	473:475	arg1	spectroscopy					477:488	13C CPMAS NMR spectroscopy	463:488	13C CPMAS NMR spectroscopy methodology	463:500	The materials before and after purification have been characterized by 13C CPMAS NMR spectroscopy methodology.					
32475571	5	22	theme	holocellulose	574:586	arg1	composition					548:558	the relative composition	535:558	the relative composition of lignin and holocellulose at each purification step	535:612	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	4	23	theme	13C	463:465	arg1	spectroscopy					477:488	13C CPMAS NMR spectroscopy	463:488	13C CPMAS NMR spectroscopy methodology	463:500	The materials before and after purification have been characterized by 13C CPMAS NMR spectroscopy methodology.					
32475571	0	24	theme	Solid	0:4	arg1	13C-NMR					12:18	Solid state 13C-NMR	0:18	Solid state 13C-NMR	0:18	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	6	25	theme	cellulosic	780:789	arg1	material					791:798	the fibrous cellulosic material	768:798	the fibrous cellulosic material	768:798	To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard.					
32475571	8	26	used	used	1084:1087	arg2	shells					1061:1066	the almond shells	1050:1066	the almond shells that are mainly used as firewood	1050:1099	These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.					
32475571	8	26	used	used	1084:1087	arg2	firewood					1092:1099	firewood	1092:1099	firewood	1092:1099	These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.					
32475571	8	27	theme	new	1038:1040	arg1	use					1042:1044	a new use	1036:1044	a new use for the almond shells that are mainly used as firewood	1036:1099	These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.					
32475571	0	28	theme	derived	111:117	arg1	materials					130:138	their derived cellulosic materials	105:138	their derived cellulosic materials	105:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	6	29	theme	fibrous	772:778	arg1	material					791:798	the fibrous cellulosic material	768:798	the fibrous cellulosic material	768:798	To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard.					
32475571	2	30	theme	D.A.	301:304	arg1	Webb					307:310	the Prunus dulcis Miller (D.A.) Webb	275:310	the Prunus dulcis Miller (D.A.) Webb	275:310	In particular, the Prunus dulcis Miller (D.A.) Webb.					
32475571	6	31	used	used	804:807	arg2	material					791:798	the fibrous cellulosic material	768:798	the fibrous cellulosic material	768:798	To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard.					
32475571	3	32	theme	available	363:371	arg1	waste					349:353	a agro industrial waste	331:353	a agro industrial waste largely available in southern Italy	331:389	was considered as a agro industrial waste largely available in southern Italy.					
32475571	1	33	theme	simple	205:210	arg1	treatment					221:229	simple alkaline treatment	205:229	simple alkaline treatment from softwood almond shells	205:257	Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood almond shells.					
32475571	0	34	theme	materials	130:138	arg1	shells					77:82	the shells	73:82	the shells of Prunus dulcis and their derived cellulosic materials	73:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	1	35	theme	alkaline	212:219	arg1	treatment					221:229	simple alkaline treatment	205:229	simple alkaline treatment from softwood almond shells	205:257	Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood almond shells.					
32475571	0	36	theme	cellulosic	119:128	arg1	materials					130:138	their derived cellulosic materials	105:138	their derived cellulosic materials	105:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	5	37	theme	relative	539:546	arg1	composition					548:558	the relative composition	535:558	the relative composition of lignin and holocellulose at each purification step	535:612	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	0	38	theme	dulcis	94:99	arg1	shells					77:82	the shells	73:82	the shells of Prunus dulcis and their derived cellulosic materials	73:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	0	39	theme	composition	50:60	arg1	studies					62:68	the cellulose composition studies	36:68	the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials	36:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	3	40	theme	industrial	338:347	arg1	waste					349:353	a agro industrial waste	331:353	a agro industrial waste largely available in southern Italy	331:389	was considered as a agro industrial waste largely available in southern Italy.					
32475571	0	41	theme	cellulose	40:48	arg1	studies					62:68	the cellulose composition studies	36:68	the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials	36:138	Solid state 13C-NMR methodology for the cellulose composition studies of the shells of Prunus dulcis and their derived cellulosic materials.					
32475571	1	42	from	shells	252:257	arg1	treatment					221:229	simple alkaline treatment	205:229	simple alkaline treatment from softwood almond shells	205:257	Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood almond shells.					
32475571	7	43	theme	packaging	924:932	arg1	purposes					934:941	packaging purposes	924:941	packaging purposes	924:941	The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material.					
32475571	5	44	theme	lignin	563:568	arg1	composition					548:558	the relative composition	535:558	the relative composition of lignin and holocellulose at each purification step	535:612	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	2	45	dep	Webb	307:310	arg1	particular					263:272	particular	263:272	particular	263:272	In particular, the Prunus dulcis Miller (D.A.) Webb.					
32475571	6	46	theme	economy	749:755	arg1	framework					757:765	a circular economy framework	738:765	a circular economy framework	738:765	To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard.					
32475571	7	47	from	performances	858:869	arg1	cardboard					887:895	the prepared cardboard	874:895	the prepared cardboard	874:895	The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material.					
32475571	5	48	theme	data	512:515	arg1	analysis					517:524	A proper data analysis	503:524	A proper data analysis	503:524	A proper data analysis provided the relative composition of lignin and holocellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR.					
32475571	1	49	theme	softwood	236:243	arg1	shells					252:257	softwood almond shells	236:257	softwood almond shells	236:257	Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood almond shells.					
32475571	6	50	theme	handmade	826:833	arg1	cardboard					835:843	a handmade cardboard	824:843	a handmade cardboard	824:843	To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard.					
32475571	1	51	theme	almond	245:250	arg1	shells					252:257	softwood almond shells	236:257	softwood almond shells	236:257	Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood almond shells.					
32475571	3	52	from	Italy	385:389	arg1	available					363:371	available	363:371	available	363:371	was considered as a agro industrial waste largely available in southern Italy.					
32475571	1	53	theme	Lignocellulosic	141:155	arg1	fibers					157:162	Lignocellulosic fibers	141:162	Lignocellulosic fibers	141:162	Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood almond shells.					
33320630	8	0	theme	tubule	1472:1477	arg1	development					1479:1489	in vitro proximal tubule development	1454:1489	in vitro proximal tubule development	1454:1489	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	2	1	theme	factors	470:476	arg1	membrane					425:432	the kidney tubular basement membrane	397:432	the kidney tubular basement membrane	397:432	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	1	theme	factors	470:476	arg1	presence					442:449	the presence	438:449	the presence of critical growth factors	438:476	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	1	2	theme	native	163:168	arg1	features					177:184	the native tissue features	159:184	the native tissue features	159:184	A successful in vitro tissue model must recapitulate the native tissue features while also being reproducible.					
33320630	8	3	theme	in	1454:1455	arg1	tubule					1472:1477	in vitro proximal tubule	1454:1477	in vitro proximal tubule development	1454:1489	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	8	4	dep	in	1454:1455	arg1	vitro					1457:1461	vitro	1457:1461	vitro	1457:1461	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	5	5	theme	sulfate	969:975	arg1	proteoglycan					977:988	heparan sulfate proteoglycan	961:988	heparan sulfate proteoglycan	961:988	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	3	6	theme	batch-to-batch	522:535	arg1	variability					537:547	batch-to-batch variability	522:547	batch-to-batch variability	522:547	However, Matrigel is not well-defined, and batch-to-batch variability is a significant issue.					
33320630	3	6	theme	batch-to-batch	522:535	arg1	issue					566:570	a significant issue	552:570	a significant issue	552:570	However, Matrigel is not well-defined, and batch-to-batch variability is a significant issue.					
33320630	2	7	theme	proximal	298:305	arg1	PCTs					327:330	PCTs	327:330	PCTs	327:330	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	7	theme	proximal	298:305	arg1	tubules					318:324	proximal convoluted tubules	298:324	proximal convoluted tubules (PCTs)	298:331	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	5	8	theme	basement	911:918	arg1	membrane					920:927	the tubular basement membrane	899:927	the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan)	899:989	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	6	9	theme	PCTs	1111:1114	arg1	formation					1098:1106	the formation	1094:1106	the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase	1094:1197	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	10	theme	key	1133:1135	arg1	E-cadherin					1156:1165	E-cadherin	1156:1165	E-cadherin	1156:1165	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	10	theme	key	1133:1135	arg1	ATPase					1192:1197	Na+/K+ ATPase	1185:1197	Na+/K+ ATPase	1185:1197	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	10	theme	key	1133:1135	arg1	aquaporin-1					1168:1178	aquaporin-1	1168:1178	aquaporin-1	1168:1178	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	10	theme	key	1133:1135	arg1	markers					1137:1143	several key markers	1125:1143	several key markers	1125:1143	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	4	11	theme	human	740:744	arg1	RPTEC/TERT1					770:780	RPTEC/TERT1	770:780	RPTEC/TERT1	770:780	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	11	theme	human	740:744	arg1	cells					763:767	immortalized human renal epithelial cells	727:767	immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1)	727:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	7	12	from	days	1289:1292	arg1	culture					1297:1303	culture	1297:1303	culture	1297:1303	Moreover, these PCT structures displayed cell polarity and a well-defined lumen after 18 days in culture.					
33320630	5	13	theme	tubular	903:909	arg1	membrane					920:927	the tubular basement membrane	899:927	the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan)	899:989	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	4	14	theme	proximal	679:686	arg1	structures					701:710	proximal tubular-like structures	679:710	proximal tubular-like structures	679:710	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	8	15	theme	kidney	1410:1415	arg1	engineering					1424:1434	kidney tissue engineering	1410:1434	kidney tissue engineering	1410:1434	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	1	16	dep	in	119:120	arg1	vitro					122:126	vitro	122:126	vitro	122:126	A successful in vitro tissue model must recapitulate the native tissue features while also being reproducible.					
33320630	1	17	theme	tissue	170:175	arg1	features					177:184	the native tissue features	159:184	the native tissue features	159:184	A successful in vitro tissue model must recapitulate the native tissue features while also being reproducible.					
33320630	5	18	theme	specific	876:883	arg1	components					885:894	specific components	876:894	specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan)	876:989	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	2	19	theme	growth	463:468	arg1	factors					470:476	critical growth factors	454:476	critical growth factors	454:476	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	4	20	theme	epithelial	752:761	arg1	RPTEC/TERT1					770:780	RPTEC/TERT1	770:780	RPTEC/TERT1	770:780	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	20	theme	epithelial	752:761	arg1	cells					763:767	immortalized human renal epithelial cells	727:767	immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1)	727:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	0	21	theme	Laminin-Entactin	14:29	arg1	Matrix					31:36	Matrigel-Free Laminin-Entactin Matrix	0:36	Matrigel-Free Laminin-Entactin Matrix	0:36	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	8	22	theme	defined	1355:1361	arg1	biomaterial					1378:1388	a defined and consistent biomaterial	1353:1388	a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies	1353:1520	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	3	23	theme	significant	554:564	arg1	variability					537:547	batch-to-batch variability	522:547	batch-to-batch variability	522:547	However, Matrigel is not well-defined, and batch-to-batch variability is a significant issue.					
33320630	3	23	theme	significant	554:564	arg1	issue					566:570	a significant issue	552:570	a significant issue	552:570	However, Matrigel is not well-defined, and batch-to-batch variability is a significant issue.					
33320630	7	24	theme	PCT	1216:1218	arg1	structures					1220:1229	these PCT structures	1210:1229	these PCT structures	1210:1229	Moreover, these PCT structures displayed cell polarity and a well-defined lumen after 18 days in culture.					
33320630	7	25	theme	well-defined	1261:1272	arg1	lumen					1274:1278	a well-defined lumen	1259:1278	a well-defined lumen	1259:1278	Moreover, these PCT structures displayed cell polarity and a well-defined lumen after 18 days in culture.					
33320630	0	26	theme	Matrigel-Free	0:12	arg1	Matrix					31:36	Matrigel-Free Laminin-Entactin Matrix	0:36	Matrigel-Free Laminin-Entactin Matrix	0:36	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	8	27	theme	nephrotoxicity	1499:1512	arg1	studies					1514:1520	nephrotoxicity studies	1499:1520	nephrotoxicity studies	1499:1520	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	8	28	theme	tissue	1417:1422	arg1	engineering					1424:1434	kidney tissue engineering	1410:1434	kidney tissue engineering	1410:1434	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	2	29	theme	tubules	318:324	arg1	formation					285:293	the formation	281:293	the formation of proximal convoluted tubules (PCTs)	281:331	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	30	theme	similar	358:364	arg1	composition					366:376	its similar composition	354:376	its similar composition	354:376	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	8	31	used	used	1402:1405	arg2	biomaterial					1378:1388	a defined and consistent biomaterial	1353:1388	a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies	1353:1520	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	5	32	theme	fibroblast	1006:1015	arg1	factor					1024:1029	fibroblast growth factor 8a	1006:1032	fibroblast growth factor 8a (FGF-8a)	1006:1041	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	5	32	theme	fibroblast	1006:1015	arg1	FGF-8a					1035:1040	FGF-8a	1035:1040	FGF-8a	1035:1040	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	2	33	theme	convoluted	307:316	arg1	PCTs					327:330	PCTs	327:330	PCTs	327:330	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	33	theme	convoluted	307:316	arg1	tubules					318:324	proximal convoluted tubules	298:324	proximal convoluted tubules (PCTs)	298:331	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	6	34	theme	Na+/K+	1185:1190	arg1	ATPase					1192:1197	Na+/K+ ATPase	1185:1197	Na+/K+ ATPase	1185:1197	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	2	35	theme	tubular	408:414	arg1	membrane					425:432	the kidney tubular basement membrane	397:432	the kidney tubular basement membrane	397:432	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	36	theme	basement	416:423	arg1	membrane					425:432	the kidney tubular basement membrane	397:432	the kidney tubular basement membrane	397:432	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	8	37	theme	proximal	1463:1470	arg1	tubule					1472:1477	in vitro proximal tubule	1454:1477	in vitro proximal tubule development	1454:1489	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	5	38	theme	membrane	920:927	arg1	components					885:894	specific components	876:894	specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan)	876:989	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	1	39	theme	successful	108:117	arg1	model					135:139	A successful in vitro tissue model	106:139	A successful in vitro tissue model	106:139	A successful in vitro tissue model must recapitulate the native tissue features while also being reproducible.					
33320630	2	40	theme	kidney	401:406	arg1	membrane					425:432	the kidney tubular basement membrane	397:432	the kidney tubular basement membrane	397:432	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	0	41	theme	Human	48:52	arg1	Tubule					69:74	Human Renal Proximal Tubule	48:74	Human Renal Proximal Tubule Structure Formation	48:94	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	7	42	theme	cell	1241:1244	arg1	polarity					1246:1253	cell polarity	1241:1253	cell polarity	1241:1253	Moreover, these PCT structures displayed cell polarity and a well-defined lumen after 18 days in culture.					
33320630	4	43	theme	murine	799:804	arg1	FOXD1					832:836	FOXD1	832:836	FOXD1	832:836	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	43	theme	murine	799:804	arg1	cells					825:829	murine fibroblast stromal cells	799:829	murine fibroblast stromal cells (FOXD1)	799:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	44	theme	laminin-entactin	621:636	arg1	L-E					639:641	L-E	639:641	L-E	639:641	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	44	theme	laminin-entactin	621:636	arg1	matrix					644:649	a laminin-entactin (L-E) matrix	619:649	a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1)	619:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	2	45	theme	critical	454:461	arg1	factors					470:476	critical growth factors	454:476	critical growth factors	454:476	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	46	with	biomaterial	254:264	arg1	membrane					425:432	the kidney tubular basement membrane	397:432	the kidney tubular basement membrane	397:432	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	46	with	biomaterial	254:264	arg1	presence					442:449	the presence	438:449	the presence of critical growth factors	438:476	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	0	47	theme	Proximal	60:67	arg1	Tubule					69:74	Human Renal Proximal Tubule	48:74	Human Renal Proximal Tubule Structure Formation	48:94	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	8	48	theme	laminin-entactin	1311:1326	arg1	matrix					1334:1339	This laminin-entactin (L-E) matrix	1306:1339	This laminin-entactin (L-E) matrix	1306:1339	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	4	49	theme	stromal	817:823	arg1	FOXD1					832:836	FOXD1	832:836	FOXD1	832:836	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	49	theme	stromal	817:823	arg1	cells					825:829	murine fibroblast stromal cells	799:829	murine fibroblast stromal cells (FOXD1)	799:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	1	50	theme	in	119:120	arg1	model					135:139	A successful in vitro tissue model	106:139	A successful in vitro tissue model	106:139	A successful in vitro tissue model must recapitulate the native tissue features while also being reproducible.					
33320630	0	51	theme	Renal	54:58	arg1	Tubule					69:74	Human Renal Proximal Tubule	48:74	Human Renal Proximal Tubule Structure Formation	48:94	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	5	52	dep	membrane	920:927	arg1	proteoglycan					977:988	heparan sulfate proteoglycan	961:988	heparan sulfate proteoglycan	961:988	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	5	52	dep	membrane	920:927	arg1	entactin/nidogen					939:954	entactin/nidogen	939:954	entactin/nidogen	939:954	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	5	52	dep	membrane	920:927	arg1	laminin					930:936	laminin	930:936	laminin	930:936	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	5	53	theme	growth	1017:1022	arg1	factor					1024:1029	fibroblast growth factor 8a	1006:1032	fibroblast growth factor 8a (FGF-8a)	1006:1041	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	5	53	theme	growth	1017:1022	arg1	FGF-8a					1035:1040	FGF-8a	1035:1040	FGF-8a	1035:1040	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	4	54	theme	fibroblast	806:815	arg1	FOXD1					832:836	FOXD1	832:836	FOXD1	832:836	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	54	theme	fibroblast	806:815	arg1	cells					825:829	murine fibroblast stromal cells	799:829	murine fibroblast stromal cells (FOXD1)	799:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	8	55	theme	consistent	1367:1376	arg1	biomaterial					1378:1388	a defined and consistent biomaterial	1353:1388	a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies	1353:1520	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	5	56	theme	heparan	961:967	arg1	proteoglycan					977:988	heparan sulfate proteoglycan	961:988	heparan sulfate proteoglycan	961:988	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	1	57	theme	tissue	128:133	arg1	model					135:139	A successful in vitro tissue model	106:139	A successful in vitro tissue model	106:139	A successful in vitro tissue model must recapitulate the native tissue features while also being reproducible.					
33320630	4	58	theme	immortalized	727:738	arg1	RPTEC/TERT1					770:780	RPTEC/TERT1	770:780	RPTEC/TERT1	770:780	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	58	theme	immortalized	727:738	arg1	cells					763:767	immortalized human renal epithelial cells	727:767	immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1)	727:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	5	59	theme	components	885:894	arg1	presence					864:871	the presence	860:871	the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan)	860:989	The matrix supports the presence of specific components of the tubular basement membrane (laminin, entactin/nidogen, and heparan sulfate proteoglycan) in addition to fibroblast growth factor 8a (FGF-8a).					
33320630	4	60	theme	renal	746:750	arg1	RPTEC/TERT1					770:780	RPTEC/TERT1	770:780	RPTEC/TERT1	770:780	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	60	theme	renal	746:750	arg1	cells					763:767	immortalized human renal epithelial cells	727:767	immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1)	727:837	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	61	theme	tubular-like	688:699	arg1	structures					701:710	proximal tubular-like structures	679:710	proximal tubular-like structures	679:710	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	4	62	theme	structures	701:710	arg1	formation					666:674	the formation	662:674	the formation of proximal tubular-like structures	662:710	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
33320630	0	63	theme	Structure	76:84	arg1	Formation					86:94	Human Renal Proximal Tubule Structure Formation	48:94	Human Renal Proximal Tubule Structure Formation	48:94	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	8	64	theme	L-E	1329:1331	arg1	matrix					1334:1339	This laminin-entactin (L-E) matrix	1306:1339	This laminin-entactin (L-E) matrix	1306:1339	This laminin-entactin (L-E) matrix constitutes a defined and consistent biomaterial that can be used in kidney tissue engineering for understanding in vitro proximal tubule development and for nephrotoxicity studies.					
33320630	6	65	theme	several	1125:1131	arg1	E-cadherin					1156:1165	E-cadherin	1156:1165	E-cadherin	1156:1165	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	65	theme	several	1125:1131	arg1	ATPase					1192:1197	Na+/K+ ATPase	1185:1197	Na+/K+ ATPase	1185:1197	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	65	theme	several	1125:1131	arg1	aquaporin-1					1168:1178	aquaporin-1	1168:1178	aquaporin-1	1168:1178	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	6	65	theme	several	1125:1131	arg1	markers					1137:1143	several key markers	1125:1143	several key markers	1125:1143	The matrix also induces tubulogenesis, leading to the formation of PCTs based on several key markers, including E-cadherin, aquaporin-1, and Na+/K+ ATPase.					
33320630	0	66	theme	Tubule	69:74	arg1	Formation					86:94	Human Renal Proximal Tubule Structure Formation	48:94	Human Renal Proximal Tubule Structure Formation	48:94	Matrigel-Free Laminin-Entactin Matrix to Induce Human Renal Proximal Tubule Structure Formation In Vitro.					
33320630	2	67	theme	principal	244:252	arg1	biomaterial					254:264	the principal biomaterial	240:264	the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro	240:340	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	2	67	theme	principal	244:252	arg1	Matrigel					228:235	Matrigel	228:235	Matrigel	228:235	Currently, Matrigel is the principal biomaterial used to induce the formation of proximal convoluted tubules (PCTs) in vitro, because of its similar composition and structure with the kidney tubular basement membrane and the presence of critical growth factors.					
33320630	4	68	theme	Matrigel-free	591:603	arg1	method					605:610	a Matrigel-free method	589:610	a Matrigel-free method	589:610	Here, we define a Matrigel-free method, using a laminin-entactin (L-E) matrix to support the formation of proximal tubular-like structures in vitro using immortalized human renal epithelial cells (RPTEC/TERT1) cocultured with murine fibroblast stromal cells (FOXD1).					
34077094	5	0	theme	mean	748:751	arg1	higher					799:804	higher	799:804	higher	799:804	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	5	0	theme	mean	748:751	arg1	BMI					753:755	the mean BMI	744:755	the mean BMI of the subfertile group	744:779	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	10	1	with	line	1572:1575	arg1	guidelines					1596:1605	the nutrition guidelines	1582:1605	the nutrition guidelines	1582:1605	However, fish, nuts and eggs consumption should be provided in line with the nutrition guidelines.					
34077094	9	2	theme	drinks	1469:1474	arg1	consumption					1438:1448	excessive consumption	1428:1448	excessive consumption of meat and sugary drinks	1428:1474	In summary, in men receiving infertility treatment, excessive consumption of meat and sugary drinks should be considered cautiously.					
34077094	1	3	theme	measurements	238:249	arg1	terms					169:173	terms	169:173	terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition	169:271	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	7	4	theme	alcohol	1094:1100	arg1	consumption					1048:1058	consumption	1048:1058	consumption of sugar sweetened beverages, and alcohol	1048:1100	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	3	5	theme	dietary	492:498	arg1	habits					500:505	dietary habits	492:505	dietary habits	492:505	Information was obtained from the participants regarding their socio-demographic characteristics, health status, dietary habits, and food intake.					
34077094	9	6	theme	excessive	1428:1436	arg1	consumption					1438:1448	excessive consumption	1428:1448	excessive consumption of meat and sugary drinks	1428:1474	In summary, in men receiving infertility treatment, excessive consumption of meat and sugary drinks should be considered cautiously.					
34077094	4	7	theme	food	556:559	arg1	records					561:567	food records	556:567	food records	556:567	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	7	8	theme	normozoospermic	1163:1177	arg1	group					1179:1183	the normozoospermic group	1159:1183	the normozoospermic group	1159:1183	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	9	9	theme	sugary	1462:1467	arg1	drinks					1469:1474	meat and sugary drinks	1453:1474	meat and sugary drinks	1453:1474	In summary, in men receiving infertility treatment, excessive consumption of meat and sugary drinks should be considered cautiously.					
34077094	7	10	theme	beverages	1079:1087	arg1	consumption					1048:1058	consumption	1048:1058	consumption of sugar sweetened beverages, and alcohol	1048:1100	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	8	11	theme	nut	1311:1313	arg1	fish					1300:1303	fish	1300:1303	fish	1300:1303	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	11	theme	nut	1311:1313	arg1	consumption					1315:1325	nut consumption	1311:1325	nut consumption	1311:1325	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	1	12	theme	normozoospermic	145:159	arg1	men					162:164	healthy (normozoospermic) men	136:164	healthy (normozoospermic) men	136:164	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	10	13	theme	fish	1518:1521	arg1	consumption					1538:1548	fish, nuts and eggs consumption	1518:1548	fish, nuts and eggs consumption	1518:1548	However, fish, nuts and eggs consumption should be provided in line with the nutrition guidelines.					
34077094	9	14	theme	meat	1453:1456	arg1	drinks					1469:1474	meat and sugary drinks	1453:1474	meat and sugary drinks	1453:1474	In summary, in men receiving infertility treatment, excessive consumption of meat and sugary drinks should be considered cautiously.					
34077094	1	15	theme	body	256:259	arg1	composition					261:271	body composition	256:271	body composition	256:271	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	4	16	theme	Statistics	680:689	arg1	programme					694:702	IBM SPSS Statistics 20 programme	671:702	IBM SPSS Statistics 20 programme	671:702	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	2	17	dep	males	289:293	arg1	subfertile					299:308	subfertile	299:308	subfertile	299:308	We included 80 males (40 subfertile and 40 healthy normozoospermic) between the ages of 25 and 54 years.					
34077094	2	17	dep	males	289:293	arg1	normozoospermic					325:339	normozoospermic	325:339	normozoospermic	325:339	We included 80 males (40 subfertile and 40 healthy normozoospermic) between the ages of 25 and 54 years.					
34077094	7	18	theme	sugar	1063:1067	arg1	beverages					1079:1087	sugar sweetened beverages	1063:1087	sugar sweetened beverages	1063:1087	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	3	19	theme	socio-demographic	442:458	arg1	characteristics					460:474	their socio-demographic characteristics	436:474	their socio-demographic characteristics	436:474	Information was obtained from the participants regarding their socio-demographic characteristics, health status, dietary habits, and food intake.					
34077094	8	20	dep	bevareges	1230:1238	arg1	consumption					1263:1273	consumption	1263:1273	sugar sweetened bevareges, red meat, organ meats consumption	1214:1273	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	10	21	theme	nutrition	1586:1594	arg1	guidelines					1596:1605	the nutrition guidelines	1582:1605	the nutrition guidelines	1582:1605	However, fish, nuts and eggs consumption should be provided in line with the nutrition guidelines.					
34077094	6	22	theme	eating	863:868	arg1	higher					892:897	higher	892:897	higher	892:897	The frequency of eating out was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	6	22	theme	eating	863:868	arg1	frequency					850:858	The frequency	846:858	The frequency of eating out	846:872	The frequency of eating out was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	7	23	theme	sweetened	1069:1077	arg1	beverages					1079:1087	sugar sweetened beverages	1063:1087	sugar sweetened beverages	1063:1087	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	3	24	theme	food	512:515	arg1	intake					517:522	food intake	512:522	food intake	512:522	Information was obtained from the participants regarding their socio-demographic characteristics, health status, dietary habits, and food intake.					
34077094	5	25	theme	study	726:730	arg1	findings					709:716	The findings	705:716	The findings of this study	705:730	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	10	26	theme	eggs	1533:1536	arg1	consumption					1538:1548	fish, nuts and eggs consumption	1518:1548	fish, nuts and eggs consumption	1518:1548	However, fish, nuts and eggs consumption should be provided in line with the nutrition guidelines.					
34077094	4	27	theme	frequency	530:538	arg1	questionnaires					540:553	Food frequency questionnaires	525:553	Food frequency questionnaires	525:553	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	8	28	theme	sugar	1214:1218	arg1	bevareges					1230:1238	sugar sweetened bevareges	1214:1238	sugar sweetened bevareges	1214:1238	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	28	theme	sugar	1214:1218	arg1	meat					1245:1248	red meat	1241:1248	red meat	1241:1248	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	28	theme	sugar	1214:1218	arg1	meats					1257:1261	organ meats	1251:1261	organ meats	1251:1261	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	0	29	theme	Nutritional	0:10	arg1	Factors					12:18	Nutritional Factors	0:18	Nutritional Factors Related to Male Fertility	0:44	Nutritional Factors Related to Male Fertility: Turkish Sample.					
34077094	1	30	theme	composition	261:271	arg1	terms					169:173	terms	169:173	terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition	169:271	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	1	31	from	difference	99:108	arg1	terms					169:173	terms	169:173	terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition	169:271	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	8	32	theme	sweetened	1220:1228	arg1	bevareges					1230:1238	sugar sweetened bevareges	1214:1238	sugar sweetened bevareges	1214:1238	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	32	theme	sweetened	1220:1228	arg1	meat					1245:1248	red meat	1241:1248	red meat	1241:1248	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	32	theme	sweetened	1220:1228	arg1	meats					1257:1261	organ meats	1251:1261	organ meats	1251:1261	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	4	33	theme	Food	525:528	arg1	questionnaires					540:553	Food frequency questionnaires	525:553	Food frequency questionnaires	525:553	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	8	34	theme	organ	1251:1255	arg1	bevareges					1230:1238	sugar sweetened bevareges	1214:1238	sugar sweetened bevareges	1214:1238	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	34	theme	organ	1251:1255	arg1	meats					1257:1261	organ meats	1251:1261	organ meats	1251:1261	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	5	35	theme	subfertile	764:773	arg1	group					775:779	the subfertile group	760:779	the subfertile group	760:779	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	9	36	theme	infertility	1405:1415	arg1	treatment					1417:1425	infertility treatment	1405:1425	infertility treatment	1405:1425	In summary, in men receiving infertility treatment, excessive consumption of meat and sugary drinks should be considered cautiously.					
34077094	0	37	theme	Related	20:26	arg1	Factors					12:18	Nutritional Factors	0:18	Nutritional Factors Related to Male Fertility	0:44	Nutritional Factors Related to Male Fertility: Turkish Sample.					
34077094	6	38	theme	normozoospermic	935:949	arg1	group					951:955	the normozoospermic group	931:955	the normozoospermic group	931:955	The frequency of eating out was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	0	39	dep	Sample	55:60	arg1	Factors					12:18	Nutritional Factors	0:18	Nutritional Factors Related to Male Fertility	0:44	Nutritional Factors Related to Male Fertility: Turkish Sample.					
34077094	1	40	theme	demographic	178:188	arg1	characteristics					190:204	demographic characteristics	178:204	demographic characteristics	178:204	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	1	41	theme	characteristics	190:204	arg1	terms					169:173	terms	169:173	terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition	169:271	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	2	42	theme	years	372:376	arg1	ages					354:357	the ages	350:357	the ages of 25 and 54 years	350:376	We included 80 males (40 subfertile and 40 healthy normozoospermic) between the ages of 25 and 54 years.					
34077094	0	43	theme	Male	31:34	arg1	Fertility					36:44	Male Fertility	31:44	Male Fertility	31:44	Nutritional Factors Related to Male Fertility: Turkish Sample.					
34077094	8	44	theme	red	1241:1243	arg1	bevareges					1230:1238	sugar sweetened bevareges	1214:1238	sugar sweetened bevareges	1214:1238	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	8	44	theme	red	1241:1243	arg1	meat					1245:1248	red meat	1241:1248	red meat	1241:1248	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	4	45	theme	sperm	621:625	arg1	analysis					627:634	sperm analysis	621:634	sperm analysis	621:634	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	0	46	theme	Turkish	47:53	arg1	Sample					55:60	Turkish Sample	47:60	Turkish Sample	47:60	Nutritional Factors Related to Male Fertility: Turkish Sample.					
34077094	3	47	theme	health	477:482	arg1	status					484:489	health status	477:489	health status	477:489	Information was obtained from the participants regarding their socio-demographic characteristics, health status, dietary habits, and food intake.					
34077094	5	48	theme	group	775:779	arg1	higher					799:804	higher	799:804	higher	799:804	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	5	48	theme	group	775:779	arg1	BMI					753:755	the mean BMI	744:755	the mean BMI of the subfertile group	744:779	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	7	49	theme	subfertile	1134:1143	arg1	group					1145:1149	the subfertile group	1130:1149	the subfertile group	1130:1149	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	6	50	theme	subfertile	906:915	arg1	group					917:921	the subfertile group	902:921	the subfertile group	902:921	The frequency of eating out was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	7	51	theme	fish	1005:1008	arg1	lower					1028:1032	lower	1028:1032	lower	1028:1032	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	7	51	theme	fish	1005:1008	arg1	consumption					990:1000	the consumption	986:1000	the consumption of fish	986:1008	It was also determined that the consumption of fish was significantly lower; in contrast, consumption of sugar sweetened beverages, and alcohol was significantly higher in the subfertile group than in the normozoospermic group.					
34077094	1	52	theme	infertile	118:126	arg1	men					128:130	infertile men	118:130	infertile men	118:130	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	1	53	theme	dietary	207:213	arg1	habits					215:220	dietary habits	207:220	dietary habits	207:220	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	8	54	theme	sperm	1358:1362	arg1	parameters					1364:1373	sperm parameters	1358:1373	sperm parameters	1358:1373	Moreover, it was found that sugar sweetened bevareges, red meat, organ meats consumption are negatively; and that fish, egg, nut consumption are positively correlated with sperm parameters.					
34077094	4	55	theme	IBM	671:673	arg1	programme					694:702	IBM SPSS Statistics 20 programme	671:702	IBM SPSS Statistics 20 programme	671:702	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	1	56	theme	habits	215:220	arg1	terms					169:173	terms	169:173	terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition	169:271	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	5	57	theme	normozoospermic	823:837	arg1	group					839:843	the normozoospermic group	819:843	the normozoospermic group	819:843	The findings of this study showed that the mean BMI of the subfertile group was significantly higher than that of the normozoospermic group.					
34077094	4	58	theme	SPSS	675:678	arg1	programme					694:702	IBM SPSS Statistics 20 programme	671:702	IBM SPSS Statistics 20 programme	671:702	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	4	59	theme	anthropometric	570:583	arg1	measurements					585:596	anthropometric measurements	570:596	anthropometric measurements	570:596	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	4	60	theme	body	599:602	arg1	composition					604:614	body composition	599:614	body composition	599:614	Food frequency questionnaires, food records, anthropometric measurements, body composition, and sperm analysis were statistically evaluated using IBM SPSS Statistics 20 programme.					
34077094	10	61	theme	nuts	1524:1527	arg1	consumption					1538:1548	fish, nuts and eggs consumption	1518:1548	fish, nuts and eggs consumption	1518:1548	However, fish, nuts and eggs consumption should be provided in line with the nutrition guidelines.					
34077094	1	62	theme	healthy	136:142	arg1	men					162:164	healthy (normozoospermic) men	136:164	healthy (normozoospermic) men	136:164	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
34077094	1	63	theme	anthropometric	223:236	arg1	measurements					238:249	anthropometric measurements	223:249	anthropometric measurements	223:249	This study aimed to investigate the difference between infertile men and healthy (normozoospermic) men in terms of demographic characteristics, dietary habits, anthropometric measurements, and body composition.					
33191780	0	0	theme	milk	92:95	arg1	oligosaccharides					97:112	human milk oligosaccharides	86:112	human milk oligosaccharides	86:112	Cross-feeding between Bifidobacterium infantis and Anaerostipes caccae on lactose and human milk oligosaccharides.					
33191780	6	1	theme	concomitant	1117:1127	arg1	production					1138:1147	concomitant butyrate production	1117:1147	concomitant butyrate production	1117:1147	A. caccae was not able to grow on these substrates except when grown in co-culture with B. infantis, leading to growth and concomitant butyrate production.					
33191780	3	2	from	diseases	560:567	arg1	infants					572:578	infants	572:578	infants	572:578	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	0	3	theme	human	86:90	arg1	oligosaccharides					97:112	human milk oligosaccharides	86:112	human milk oligosaccharides	86:112	Cross-feeding between Bifidobacterium infantis and Anaerostipes caccae on lactose and human milk oligosaccharides.					
33191780	3	4	theme	colicky	532:538	arg1	symptoms					540:547	colicky symptoms	532:547	colicky symptoms	532:547	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	7	5	theme	liberated	1225:1233	arg1	monosaccharides					1235:1249	the liberated monosaccharides	1221:1249	the liberated monosaccharides as well as lactate and acetate produced by B. infantis	1221:1304	Two levels of cross-feeding were observed, in which A. caccae utilised the liberated monosaccharides as well as lactate and acetate produced by B. infantis.					
33191780	8	6	theme	bifidobacteria	1378:1391	arg1	role					1370:1373	the key ecological role	1351:1373	the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut	1351:1477	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	8	7	theme	infant	1468:1473	arg1	gut					1475:1477	the infant gut	1464:1477	the infant gut	1464:1477	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	2	8	theme	milk	292:295	arg1	substrates					339:348	specific substrates	330:348	specific substrates	330:348	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	2	8	theme	milk	292:295	arg1	HMOs					315:318	HMOs	315:318	HMOs	315:318	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	2	8	theme	milk	292:295	arg1	oligosaccharides					297:312	human milk oligosaccharides	286:312	human milk oligosaccharides (HMOs)	286:319	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	8	9	theme	other	1421:1425	arg1	species					1437:1443	other important species	1421:1443	other important species that will colonise the infant gut	1421:1477	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	7	10	theme	B.	1294:1295	arg1	infantis					1297:1304	B. infantis	1294:1304	B. infantis	1294:1304	Two levels of cross-feeding were observed, in which A. caccae utilised the liberated monosaccharides as well as lactate and acetate produced by B. infantis.					
33191780	3	11	from	symptoms	540:547	arg1	infants					572:578	infants	572:578	infants	572:578	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	2	12	theme	human	286:290	arg1	substrates					339:348	specific substrates	330:348	specific substrates	330:348	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	2	12	theme	human	286:290	arg1	HMOs					315:318	HMOs	315:318	HMOs	315:318	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	2	12	theme	human	286:290	arg1	oligosaccharides					297:312	human milk oligosaccharides	286:312	human milk oligosaccharides (HMOs)	286:319	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	4	13	theme	trophic	600:606	arg1	interactions					608:619	the trophic interactions	596:619	the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates	596:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	8	14	theme	microbial	1312:1320	arg1	points					1336:1341	This microbial cross-feeding points	1307:1341	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.	1307:1478	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	3	15	theme	atopic	553:558	arg1	diseases					560:567	atopic diseases	553:567	atopic diseases	553:567	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	5	16	theme	human	968:972	arg1	carbohydrates					979:991	total human milk carbohydrates	962:991	total human milk carbohydrates	962:991	Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates.					
33191780	2	17	theme	nursling	385:392	arg1	microbiota					367:376	the gut microbiota	359:376	the gut microbiota of the nursling	359:392	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	4	18	theme	human	812:816	arg1	carbohydrates					823:835	total human milk carbohydrates	806:835	total human milk carbohydrates	806:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	1	19	theme	dynamic	184:190	arg1	process					192:198	a dynamic process	182:198	a dynamic process that may impact lifelong health	182:230	The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health.					
33191780	1	19	theme	dynamic	184:190	arg1	establishment					119:131	The establishment	115:131	The establishment of the gut microbiota immediately after birth	115:177	The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health.					
33191780	9	20	dep	crosstalk	1629:1637	arg1	maturation					1647:1656	maturation	1647:1656	maturation	1647:1656	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	9	21	theme	host-microbial	1614:1627	arg1	crosstalk					1629:1637	crosstalk	1629:1637	crosstalk	1629:1637	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	9	22	theme	gut	1509:1511	arg1	composition					1524:1534	the gut microbiota composition	1505:1534	the gut microbiota composition that contributes to the gradual production of butyrate	1505:1589	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	4	23	from	period	777:782	arg1	relevant					750:757	relevant	750:757	relevant	750:757	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	4	24	theme	life	772:775	arg1	period					777:782	the early life period	762:782	the early life period including lactose and total human milk carbohydrates	762:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	2	25	from	stage	265:269	arg1	life					280:283	early life	274:283	early life	274:283	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	5	26	theme	anaerobic	911:919	arg1	bioreactors					921:931	anaerobic bioreactors	911:931	anaerobic bioreactors supplemented with lactose or total human milk carbohydrates	911:991	Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates.					
33191780	5	27	theme	milk	974:977	arg1	carbohydrates					979:991	total human milk carbohydrates	962:991	total human milk carbohydrates	962:991	Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates.					
33191780	8	28	theme	important	1427:1435	arg1	species					1437:1443	other important species	1421:1443	other important species that will colonise the infant gut	1421:1477	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	5	29	theme	species	879:885	arg1	Mono-					838:842	Mono-	838:842	Mono-	838:842	Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates.					
33191780	5	30	theme	bacterial	869:877	arg1	species					879:885	these bacterial species	863:885	these bacterial species	863:885	Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates.					
33191780	4	31	theme	milk	818:821	arg1	carbohydrates					823:835	total human milk carbohydrates	806:835	total human milk carbohydrates	806:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	8	32	theme	ecological	1359:1368	arg1	role					1370:1373	the key ecological role	1351:1373	the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut	1351:1477	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	9	33	theme	butyrate	1582:1589	arg1	production					1568:1577	the gradual production	1556:1577	the gradual production of butyrate	1556:1589	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	8	34	theme	key	1355:1357	arg1	role					1370:1373	the key ecological role	1351:1373	the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut	1351:1477	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	3	35	theme	well-orchestrated	399:415	arg1	important					431:439	important	431:439	important	431:439	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	3	35	theme	well-orchestrated	399:415	arg1	transition					417:426	The well-orchestrated transition	395:426	The well-orchestrated transition	395:426	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	4	36	theme	total	806:810	arg1	carbohydrates					823:835	total human milk carbohydrates	806:835	total human milk carbohydrates	806:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	9	37	theme	microbiota	1513:1522	arg1	composition					1524:1534	the gut microbiota composition	1505:1534	the gut microbiota composition that contributes to the gradual production of butyrate	1505:1589	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	2	38	theme	important	241:249	arg1	stage					265:269	this important developmental stage	236:269	this important developmental stage in early life	236:283	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	3	39	theme	aberrant	447:454	arg1	composition					466:476	an aberrant microbial composition	444:476	an aberrant microbial composition	444:476	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	5	40	theme	total	962:966	arg1	carbohydrates					979:991	total human milk carbohydrates	962:991	total human milk carbohydrates	962:991	Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates.					
33191780	3	41	theme	microbial	456:464	arg1	composition					466:476	an aberrant microbial composition	444:476	an aberrant microbial composition	444:476	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	7	42	theme	cross-feeding	1164:1176	arg1	levels					1154:1159	Two levels	1150:1159	Two levels of cross-feeding	1150:1176	Two levels of cross-feeding were observed, in which A. caccae utilised the liberated monosaccharides as well as lactate and acetate produced by B. infantis.					
33191780	9	43	theme	progressive	1484:1494	arg1	shift					1496:1500	The progressive shift	1480:1500	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate	1480:1589	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	9	43	theme	progressive	1484:1494	arg1	important					1600:1608	important	1600:1608	important	1600:1608	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	3	44	link	bacterial-derived	482:498	arg1	metabolites					500:510	bacterial-derived metabolites	482:510	bacterial-derived metabolites	482:510	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	6	45	dep	A.	994:995	arg1	caccae					997:1002	A. caccae	994:1002	A. caccae	994:1002	A. caccae was not able to grow on these substrates except when grown in co-culture with B. infantis, leading to growth and concomitant butyrate production.					
33191780	4	46	dep	infantis	662:669	arg1	an					629:630	an	629:630	an	629:630	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	8	47	theme	cross-feeding	1322:1334	arg1	points					1336:1341	This microbial cross-feeding points	1307:1341	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.	1307:1478	This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut.					
33191780	2	48	theme	specific	330:337	arg1	substrates					339:348	specific substrates	330:348	specific substrates	330:348	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	2	48	theme	specific	330:337	arg1	oligosaccharides					297:312	human milk oligosaccharides	286:312	human milk oligosaccharides (HMOs)	286:319	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	6	49	with	co-culture	1066:1075	arg1	infantis					1085:1092	B. infantis	1082:1092	B. infantis	1082:1092	A. caccae was not able to grow on these substrates except when grown in co-culture with B. infantis, leading to growth and concomitant butyrate production.					
33191780	2	50	theme	early	274:278	arg1	life					280:283	early life	274:283	early life	274:283	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	1	51	theme	gut	140:142	arg1	microbiota					144:153	the gut microbiota	136:153	the gut microbiota	136:153	The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health.					
33191780	4	52	theme	early	766:770	arg1	period					777:782	the early life period	762:782	the early life period including lactose and total human milk carbohydrates	762:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	1	53	theme	microbiota	144:153	arg1	process					192:198	a dynamic process	182:198	a dynamic process that may impact lifelong health	182:230	The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health.					
33191780	1	53	theme	microbiota	144:153	arg1	establishment					119:131	The establishment	115:131	The establishment of the gut microbiota immediately after birth	115:177	The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health.					
33191780	3	54	theme	bacterial-derived	482:498	arg1	metabolites					500:510	bacterial-derived metabolites	482:510	bacterial-derived metabolites	482:510	The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants.					
33191780	9	55	theme	composition	1524:1534	arg1	shift					1496:1500	The progressive shift	1480:1500	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate	1480:1589	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	9	55	theme	composition	1524:1534	arg1	important					1600:1608	important	1600:1608	important	1600:1608	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	6	56	theme	butyrate	1129:1136	arg1	production					1138:1147	concomitant butyrate production	1117:1147	concomitant butyrate production	1117:1147	A. caccae was not able to grow on these substrates except when grown in co-culture with B. infantis, leading to growth and concomitant butyrate production.					
33191780	9	57	theme	gradual	1560:1566	arg1	production					1568:1577	the gradual production	1556:1577	the gradual production of butyrate	1556:1589	The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation.					
33191780	2	58	theme	gut	363:365	arg1	microbiota					367:376	the gut microbiota	359:376	the gut microbiota of the nursling	359:392	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	4	59	from	relevant	750:757	arg1	period					777:782	the early life period	762:782	the early life period including lactose and total human milk carbohydrates	762:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
33191780	2	60	theme	developmental	251:263	arg1	stage					265:269	this important developmental stage	236:269	this important developmental stage in early life	236:283	At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling.					
33191780	4	61	theme	carbohydrate	717:728	arg1	substrates					730:739	carbohydrate substrates	717:739	carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates	717:835	Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates.					
32422624	4	0	theme	physical	518:525	arg1	properties					573:582	in vitro and in vivo properties	552:582	in vitro and in vivo properties of SA-BBG composite wound dressing	552:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	4	0	theme	physical	518:525	arg1	properties					540:549	The physical and chemical properties	514:549	The physical and chemical properties	514:549	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	10	1	theme	composite	1169:1177	arg1	dressing					1179:1186	the SA-BBG composite dressing	1158:1186	the SA-BBG composite dressing	1158:1186	In conclusion, the SA-BBG composite dressing shows great potential for application in skin wound healing and SA3BBG works best.					
32422624	5	2	theme	absorption	716:725	arg1	performance					727:737	good water absorption performance	705:737	good water absorption performance	705:737	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	1	3	theme	serious	195:201	arg1	problem					228:234	a serious and intractable clinical problem	193:234	a serious and intractable clinical problem	193:234	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	1	3	theme	serious	195:201	arg1	injury					183:188	Full-thickness skin injury	163:188	Full-thickness skin injury	163:188	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	7	4	theme	skin	929:932	arg1	defects					934:940	full-thickness skin defects	914:940	full-thickness skin defects in rats	914:948	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	4	5	theme	composite	594:602	arg1	dressing					610:617	SA-BBG composite wound dressing	587:617	SA-BBG composite wound dressing	587:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	4	6	dep	in	552:553	arg1	vitro					555:559	vitro	555:559	vitro	555:559	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	5	7	theme	SA-BBG	669:674	arg1	dressing					686:693	the SA-BBG composite dressing	665:693	the SA-BBG composite dressing	665:693	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	6	8	from	ions	762:765	arg1	BBG					770:772	BBG	770:772	BBG	770:772	The boron and silicon ions in BBG can maintain stable and sustained release.					
32422624	4	9	theme	SA-BBG	587:592	arg1	dressing					610:617	SA-BBG composite wound dressing	587:617	SA-BBG composite wound dressing	587:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	0	10	theme	skin	143:146	arg1	healing					154:160	full-thickness skin wound healing	128:160	full-thickness skin wound healing	128:160	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	4	11	theme	chemical	531:538	arg1	properties					573:582	in vitro and in vivo properties	552:582	in vitro and in vivo properties of SA-BBG composite wound dressing	552:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	4	11	theme	chemical	531:538	arg1	properties					540:549	The physical and chemical properties	514:549	The physical and chemical properties	514:549	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	5	12	theme	composite	676:684	arg1	dressing					686:693	the SA-BBG composite dressing	665:693	the SA-BBG composite dressing	665:693	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	9	13	from	bioglass	1074:1081	arg1	sponge					1090:1095	the sponge	1086:1095	the sponge	1086:1095	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	8	14	theme	dressing	992:999	arg1	groups					1001:1006	SA-BBG composite dressing groups	975:1006	SA-BBG composite dressing groups	975:1006	The wounds treated with SA-BBG composite dressing groups had almost closed at day 15.					
32422624	4	15	theme	dressing	610:617	arg1	properties					573:582	in vitro and in vivo properties	552:582	in vitro and in vivo properties of SA-BBG composite wound dressing	552:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	4	15	theme	dressing	610:617	arg1	properties					540:549	The physical and chemical properties	514:549	The physical and chemical properties	514:549	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	0	16	theme	full-thickness	128:141	arg1	healing					154:160	full-thickness skin wound healing	128:160	full-thickness skin wound healing	128:160	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	10	17	from	application	1214:1224	arg1	healing					1240:1246	skin wound healing	1229:1246	skin wound healing	1229:1246	In conclusion, the SA-BBG composite dressing shows great potential for application in skin wound healing and SA3BBG works best.					
32422624	9	18	theme	wound	1109:1113	arg1	healing					1115:1121	wound healing	1109:1121	the wound healing effect	1105:1128	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	6	19	theme	boron	744:748	arg1	ions					762:765	The boron and silicon ions	740:765	ions	762:765	The boron and silicon ions in BBG can maintain stable and sustained release.					
32422624	4	20	theme	wound	604:608	arg1	dressing					610:617	SA-BBG composite wound dressing	587:617	SA-BBG composite wound dressing	587:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	7	21	theme	wound	889:893	arg1	healing					895:901	outstanding wound healing	877:901	outstanding wound healing ability	877:909	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	7	22	theme	healing	895:901	arg1	ability					903:909	outstanding wound healing ability	877:909	outstanding wound healing ability	877:909	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	1	23	theme	intractable	207:217	arg1	problem					228:234	a serious and intractable clinical problem	193:234	a serious and intractable clinical problem	193:234	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	1	23	theme	intractable	207:217	arg1	injury					183:188	Full-thickness skin injury	163:188	Full-thickness skin injury	163:188	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	0	24	theme	wound	148:152	arg1	healing					154:160	full-thickness skin wound healing	128:160	full-thickness skin wound healing	128:160	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	0	25	theme	sodium	71:76	arg1	dressing					103:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	10	26	theme	great	1194:1198	arg1	potential					1200:1208	great potential	1194:1208	great potential for application in skin wound healing	1194:1246	In conclusion, the SA-BBG composite dressing shows great potential for application in skin wound healing and SA3BBG works best.					
32422624	2	27	theme	skin	311:314	arg1	reconstruction					316:329	skin reconstruction	311:329	skin reconstruction	311:329	Wound dressing is urgently needed to treat serious skin defects or induce skin reconstruction.					
32422624	10	28	theme	wound	1234:1238	arg1	healing					1240:1246	skin wound healing	1229:1246	skin wound healing	1229:1246	In conclusion, the SA-BBG composite dressing shows great potential for application in skin wound healing and SA3BBG works best.					
32422624	1	29	theme	clinical	219:226	arg1	problem					228:234	a serious and intractable clinical problem	193:234	a serious and intractable clinical problem	193:234	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	1	29	theme	clinical	219:226	arg1	injury					183:188	Full-thickness skin injury	163:188	Full-thickness skin injury	163:188	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	3	30	theme	alginate	418:425	arg1	dressing					438:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing	368:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing	368:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	4	31	dep	in	565:566	arg1	vivo					568:571	vivo	568:571	vivo	568:571	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	7	32	from	defects	934:940	arg1	rats					945:948	rats	945:948	rats	945:948	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	7	33	theme	outstanding	877:887	arg1	healing					895:901	outstanding wound healing	877:901	outstanding wound healing ability	877:909	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	6	34	theme	sustained	798:806	arg1	release					808:814	stable and sustained release	787:814	stable and sustained release	787:814	The boron and silicon ions in BBG can maintain stable and sustained release.					
32422624	7	35	theme	wound	856:860	arg1	dressing					862:869	the SA-BBG composite wound dressing	835:869	the SA-BBG composite wound dressing	835:869	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	3	36	theme	-incorporated	397:409	arg1	dressing					438:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing	368:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing	368:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	4	37	theme	in	565:566	arg1	properties					573:582	in vitro and in vivo properties	552:582	in vitro and in vivo properties of SA-BBG composite wound dressing	552:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	4	37	theme	in	565:566	arg1	properties					540:549	The physical and chemical properties	514:549	The physical and chemical properties	514:549	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	3	38	theme	sodium	411:416	arg1	dressing					438:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing	368:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing	368:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	9	39	theme	healing	1115:1121	arg1	effect					1123:1128	the wound healing effect	1105:1128	the wound healing effect	1105:1128	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	9	39	theme	healing	1115:1121	arg1	best					1137:1140	best	1137:1140	best	1137:1140	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	7	40	theme	SA-BBG	839:844	arg1	dressing					862:869	the SA-BBG composite wound dressing	835:869	the SA-BBG composite wound dressing	835:869	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	3	41	theme	accelerated	487:497	arg1	healing					505:511	accelerated wound healing	487:511	accelerated wound healing	487:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	3	42	theme	wound	432:436	arg1	dressing					438:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing	368:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing	368:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	2	43	theme	serious	280:286	arg1	defects					293:299	serious skin defects	280:299	serious skin defects	280:299	Wound dressing is urgently needed to treat serious skin defects or induce skin reconstruction.					
32422624	7	44	theme	composite	846:854	arg1	dressing					862:869	the SA-BBG composite wound dressing	835:869	the SA-BBG composite wound dressing	835:869	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
32422624	3	45	theme	effective	463:471	arg1	technique					473:481	a simple and effective technique	450:481	a simple and effective technique for accelerated wound healing	450:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	0	46	theme	borosilicate-bioglass-incorporated	36:69	arg1	dressing					103:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	4	47	theme	in	552:553	arg1	properties					573:582	in vitro and in vivo properties	552:582	in vitro and in vivo properties of SA-BBG composite wound dressing	552:617	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	4	47	theme	in	552:553	arg1	properties					540:549	The physical and chemical properties	514:549	The physical and chemical properties	514:549	The physical and chemical properties, in vitro and in vivo properties of SA-BBG composite wound dressing have been investigated.					
32422624	6	48	theme	stable	787:792	arg1	release					808:814	stable and sustained release	787:814	stable and sustained release	787:814	The boron and silicon ions in BBG can maintain stable and sustained release.					
32422624	3	49	theme	SA	428:429	arg1	dressing					438:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing	368:445	a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing	368:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	0	50	theme	wound	97:101	arg1	dressing					103:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	0	51	theme	alginate	78:85	arg1	dressing					103:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	5	52	theme	good	705:708	arg1	performance					727:737	good water absorption performance	705:737	good water absorption performance	705:737	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	1	53	theme	Full-thickness	163:176	arg1	problem					228:234	a serious and intractable clinical problem	193:234	a serious and intractable clinical problem	193:234	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	1	53	theme	Full-thickness	163:176	arg1	injury					183:188	Full-thickness skin injury	163:188	Full-thickness skin injury	163:188	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	3	54	theme	simple	452:457	arg1	technique					473:481	a simple and effective technique	450:481	a simple and effective technique for accelerated wound healing	450:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	5	55	contain	possesses	695:703	arg2	performance					727:737	good water absorption performance	705:737	good water absorption performance	705:737	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	5	55	contain	possesses	695:703	arg1	dressing					686:693	the SA-BBG composite dressing	665:693	the SA-BBG composite dressing	665:693	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	5	56	theme	water	710:714	arg1	performance					727:737	good water absorption performance	705:737	good water absorption performance	705:737	The results show that the SA-BBG composite dressing possesses good water absorption performance.					
32422624	1	57	theme	skin	178:181	arg1	problem					228:234	a serious and intractable clinical problem	193:234	a serious and intractable clinical problem	193:234	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	1	57	theme	skin	178:181	arg1	injury					183:188	Full-thickness skin injury	163:188	Full-thickness skin injury	163:188	Full-thickness skin injury is a serious and intractable clinical problem.					
32422624	10	58	theme	skin	1229:1232	arg1	healing					1240:1246	skin wound healing	1229:1246	skin wound healing	1229:1246	In conclusion, the SA-BBG composite dressing shows great potential for application in skin wound healing and SA3BBG works best.					
32422624	9	59	theme	alginate	1062:1069	arg1	3:1					1100:1102	3:1	1100:1102	3:1	1100:1102	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	9	59	theme	alginate	1062:1069	arg1	ratio					1046:1050	the ratio	1042:1050	the ratio of sodium alginate to bioglass in the sponge	1042:1095	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	3	60	theme	first	340:344	arg1	time					346:349	the first time	336:349	the first time	336:349	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	8	61	theme	composite	982:990	arg1	groups					1001:1006	SA-BBG composite dressing groups	975:1006	SA-BBG composite dressing groups	975:1006	The wounds treated with SA-BBG composite dressing groups had almost closed at day 15.					
32422624	3	62	theme	wound	499:503	arg1	healing					505:511	accelerated wound healing	487:511	accelerated wound healing	487:511	For the first time, we demonstrated a borosilicate bioglass (BBG)-incorporated sodium alginate (SA) wound dressing by a simple and effective technique for accelerated wound healing.					
32422624	0	63	theme	dressing	103:110	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	0	63	theme	dressing	103:110	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	2	64	theme	Wound	237:241	arg1	dressing					243:250	Wound dressing	237:250	Wound dressing	237:250	Wound dressing is urgently needed to treat serious skin defects or induce skin reconstruction.					
32422624	8	65	theme	SA-BBG	975:980	arg1	groups					1001:1006	SA-BBG composite dressing groups	975:1006	SA-BBG composite dressing groups	975:1006	The wounds treated with SA-BBG composite dressing groups had almost closed at day 15.					
32422624	9	66	theme	sodium	1055:1060	arg1	alginate					1062:1069	sodium alginate	1055:1069	sodium alginate	1055:1069	When the ratio of sodium alginate to bioglass in the sponge is 3:1, the wound healing effect is the best.					
32422624	10	67	theme	SA-BBG	1162:1167	arg1	dressing					1179:1186	the SA-BBG composite dressing	1158:1186	the SA-BBG composite dressing	1158:1186	In conclusion, the SA-BBG composite dressing shows great potential for application in skin wound healing and SA3BBG works best.					
32422624	0	68	theme	composite	87:95	arg1	dressing					103:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	borosilicate-bioglass-incorporated sodium alginate composite wound dressing	36:110	Preparation and characterization of borosilicate-bioglass-incorporated sodium alginate composite wound dressing for accelerated full-thickness skin wound healing.					
32422624	2	69	theme	skin	288:291	arg1	defects					293:299	serious skin defects	280:299	serious skin defects	280:299	Wound dressing is urgently needed to treat serious skin defects or induce skin reconstruction.					
32422624	6	70	theme	silicon	754:760	arg1	ions					762:765	The boron and silicon ions	740:765	ions	762:765	The boron and silicon ions in BBG can maintain stable and sustained release.					
32422624	7	71	theme	full-thickness	914:927	arg1	defects					934:940	full-thickness skin defects	914:940	full-thickness skin defects in rats	914:948	Most importantly, the SA-BBG composite wound dressing shows outstanding wound healing ability in full-thickness skin defects in rats.					
33787105	5	0	theme	drug	1315:1318	arg1	SLB-SNEDDS					1336:1345	SLB-SNEDDS	1336:1345	SLB-SNEDDS	1336:1345	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	0	theme	drug	1315:1318	arg1	system					1329:1334	SLB self-nanoemulsion drug delivery system	1293:1334	SLB self-nanoemulsion drug delivery system(SLB-SNEDDS)	1293:1346	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	2	1	theme	cell	347:350	arg1	toxicity					352:359	The cell toxicity	343:359	The cell toxicity of self-emulsifying preparation	343:391	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	1	2	theme	supersaturable	232:245	arg1	system					282:287	supersaturable self-nanoemulsifying drug delivery system	232:287	supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	232:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	1	3	theme	drug	268:271	arg1	system					282:287	supersaturable self-nanoemulsifying drug delivery system	232:287	supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	232:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	8	4	theme	SLB	2144:2146	arg1	absorption					2130:2139	absorption	2130:2139	absorption	2130:2139	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	8	4	theme	SLB	2144:2146	arg1	transport					2116:2124	transport	2116:2124	transport	2116:2124	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	8	5	theme	transport	1989:1997	arg1	process					1999:2005	the osmotic transport process	1977:2005	the osmotic transport process of SLB	1977:2012	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	6	6	from	times	1553:1557	arg1	group					1613:1617	SLB suspension group	1598:1617	SLB suspension group	1598:1617	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	3	7	dep	in	599:600	arg1	vivo					602:605	vivo	602:605	vivo	602:605	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	6	8	with	times	1568:1572	arg1	bioavailability					1636:1650	a relative bioavailability	1625:1650	a relative bioavailability of 578.45% and 1 139.44% respectively	1625:1688	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	1	9	from	system	282:287	arg1	absorption					200:209	absorption	200:209	absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	200:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	5	10	theme	SLB	1293:1295	arg1	SLB-SNEDDS					1336:1345	SLB-SNEDDS	1336:1345	SLB-SNEDDS	1336:1345	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	10	theme	SLB	1293:1295	arg1	system					1329:1334	SLB self-nanoemulsion drug delivery system	1293:1334	SLB self-nanoemulsion drug delivery system(SLB-SNEDDS)	1293:1346	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	8	11	theme	oral	2163:2166	arg1	bioavailability					2168:2182	the oral bioavailability	2159:2182	the oral bioavailability of SLB	2159:2189	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	2	12	theme	Caco-2	566:571	arg1	model					588:592	a Caco-2 cell monolayer model	564:592	a Caco-2 cell monolayer model	564:592	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	6	13	theme	SLB	1381:1383	arg1	suspension					1385:1394	SLB suspension	1381:1394	SLB suspension	1381:1394	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	5	14	theme	transport	1084:1092	arg1	rate					1094:1097	the transport rate	1080:1097	the transport rate of silybin	1080:1108	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	6	15	theme	SLB-SSNEDDS	1483:1493	arg1	groups					1495:1500	both SLB-SNEDDS and SLB-SSNEDDS groups	1463:1500	both SLB-SNEDDS and SLB-SSNEDDS groups	1463:1500	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	4	16	theme	Caco-2	826:831	arg1	cells					833:837	Caco-2 cells	826:837	Caco-2 cells	826:837	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	6	17	from	group	1613:1617	arg1	times					1553:1557	5.25 times	1548:1557	5.25 times	1548:1557	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	6	17	from	group	1613:1617	arg1	times					1568:1572	9.69 times	1563:1572	9.69 times respectively	1563:1585	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	3	18	theme	in	599:600	arg1	correlation					636:646	The in vivo and in vitro supersaturation correlation	595:646	The in vivo and in vitro supersaturation correlation	595:646	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	6	19	theme	SLB-SNEDDS	1468:1477	arg1	groups					1495:1500	both SLB-SNEDDS and SLB-SSNEDDS groups	1463:1500	both SLB-SNEDDS and SLB-SSNEDDS groups	1463:1500	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	2	20	theme	monolayer	578:586	arg1	model					588:592	a Caco-2 cell monolayer model	564:592	a Caco-2 cell monolayer model	564:592	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	5	21	theme	transport	962:970	arg1	results					972:978	the transport results	958:978	the transport results	958:978	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	6	22	theme	blood	1440:1444	arg1	concentration					1446:1458	the blood concentration	1436:1458	the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups	1436:1500	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	1	23	theme	Hydroxypropyl	138:150	arg1	succinate					176:184	Hydroxypropyl methylcellulose acetate succinate	138:184	Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF)	138:195	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	1	23	theme	Hydroxypropyl	138:150	arg1	MF					193:194	HPMCAS MF	186:194	HPMCAS MF	186:194	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	4	24	theme	cells	904:908	arg1	viability					910:918	Caco-2 cells viability	897:918	Caco-2 cells viability	897:918	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	0	25	theme	self-nanoemulsifying	67:86	arg1	system					102:107	supersaturable self-nanoemulsifying drug delivery system	52:107	supersaturable self-nanoemulsifying drug delivery system	52:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	1	26	theme	acetate	168:174	arg1	succinate					176:184	Hydroxypropyl methylcellulose acetate succinate	138:184	Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF)	138:195	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	1	26	theme	acetate	168:174	arg1	MF					193:194	HPMCAS MF	186:194	HPMCAS MF	186:194	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	0	27	from	[Effects	0:7	arg1	absorption					25:34	absorption	25:34	absorption of silybin from supersaturable self-nanoemulsifying drug delivery system	25:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	0	28	theme	delivery	93:100	arg1	system					102:107	supersaturable self-nanoemulsifying drug delivery system	52:107	supersaturable self-nanoemulsifying drug delivery system	52:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	7	29	theme	those	1771:1775	arg1	those					1771:1775	those	1771:1775	those	1771:1775	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	7	29	theme	those	1771:1775	arg1	times					1753:1757	1.85 times and 197%	1748:1766	times	1753:1757	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	7	29	theme	those	1771:1775	arg1	%					1766:1766	1.85 times and 197%	1748:1766	%	1766:1766	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	2	30	theme	preparation	521:531	arg1	permeability					452:463	the in vitro membrane permeability	430:463	the in vitro membrane permeability	430:463	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	2	30	theme	preparation	521:531	arg1	effect					490:495	absorption promoting effect	469:495	absorption promoting effect of the self-emulsifying preparation	469:531	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	6	31	theme	that	1590:1593	arg1	times					1553:1557	5.25 times	1548:1557	5.25 times	1548:1557	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	6	31	theme	that	1590:1593	arg1	times					1568:1572	9.69 times	1563:1572	9.69 times respectively	1563:1585	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	5	32	theme	supersaturated	1185:1198	arg1	transport					1200:1208	supersaturated transport	1185:1208	supersaturated transport	1185:1208	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	8	33	theme	SLB	1873:1875	arg1	solubility					1859:1868	the solubility	1855:1868	the solubility of SLB in gastrointestinal tract	1855:1901	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	1	34	theme	delivery	273:280	arg1	system					282:287	supersaturable self-nanoemulsifying drug delivery system	232:287	supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	232:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	2	35	dep	in	434:435	arg1	vitro					437:441	vitro	437:441	vitro	437:441	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	8	36	theme	gastrointestinal	1880:1895	arg1	tract					1897:1901	gastrointestinal tract	1880:1901	gastrointestinal tract	1880:1901	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	6	37	from	times	1568:1572	arg1	group					1613:1617	SLB suspension group	1598:1617	SLB suspension group	1598:1617	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	6	38	theme	%	1661:1661	arg1	bioavailability					1636:1650	a relative bioavailability	1625:1650	a relative bioavailability of 578.45% and 1 139.44% respectively	1625:1688	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	4	39	theme	preparation	754:764	arg1	concentration					733:745	the concentration	729:745	the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1))	729:807	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	4	39	theme	preparation	754:764	arg1	toxic					817:821	toxic	817:821	toxic	817:821	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	0	40	theme	HPMCAS	12:17	arg1	MF					19:20	HPMCAS MF	12:20	HPMCAS MF	12:20	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	2	41	theme	absorption	469:478	arg1	effect					490:495	absorption promoting effect	469:495	absorption promoting effect of the self-emulsifying preparation	469:531	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	4	42	theme	SLB	788:790	arg1	μg·mL~					797:802	C_(SLB) 100 μg·mL~(-1)	785:806	C_(SLB) 100 μg·mL~(-1)	785:806	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	4	42	theme	SLB	788:790	arg1	mg·mL~					774:779	2 mg·mL~	772:779	2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1))	772:807	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	2	43	theme	MTT	414:416	arg1	method					418:423	the MTT method	410:423	the MTT method	410:423	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	5	44	theme	self-emulsifying	1018:1033	arg1	preparation					1035:1045	the self-emulsifying preparation	1014:1045	the self-emulsifying preparation	1014:1045	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	7	45	theme	SLB-SSNEDDS	1731:1741	arg1	C_					1691:1692	C_	1691:1692	C_(max)	1691:1697	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	7	45	theme	SLB-SSNEDDS	1731:1741	arg1	bioavailability					1712:1726	relative bioavailability	1703:1726	relative bioavailability of SLB-SSNEDDS	1703:1741	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	7	45	theme	SLB-SSNEDDS	1731:1741	arg1	max					1694:1696	max	1694:1696	max	1694:1696	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	6	46	theme	relative	1627:1634	arg1	bioavailability					1636:1650	a relative bioavailability	1625:1650	a relative bioavailability of 578.45% and 1 139.44% respectively	1625:1688	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	8	47	theme	SLB	1931:1933	arg1	state					1951:1955	SLB supersaturation state	1931:1955	SLB supersaturation state	1931:1955	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	3	48	theme	SLB	693:695	arg1	concentration					676:688	the blood concentration	666:688	the blood concentration of SLB	666:695	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	5	49	theme	silybin	1102:1108	arg1	rate					1094:1097	the transport rate	1080:1097	the transport rate of silybin	1080:1108	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	8	50	theme	state	1951:1955	arg1	stability					1918:1926	stability	1918:1926	stability of SLB supersaturation state	1918:1955	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	4	51	theme	C_	785:786	arg1	μg·mL~					797:802	C_(SLB) 100 μg·mL~(-1)	785:806	C_(SLB) 100 μg·mL~(-1)	785:806	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	4	51	theme	C_	785:786	arg1	mg·mL~					774:779	2 mg·mL~	772:779	2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1))	772:807	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	5	52	theme	SLB	1233:1235	arg1	system					1269:1274	supersaturated SLB self-nanoemulsion drug delivery system	1218:1274	supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS)	1218:1287	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	52	theme	SLB	1233:1235	arg1	SLB-SSNEDDS					1276:1286	SLB-SSNEDDS	1276:1286	SLB-SSNEDDS	1276:1286	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	2	53	theme	self-emulsifying	364:379	arg1	preparation					381:391	self-emulsifying preparation	364:391	self-emulsifying preparation	364:391	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	5	54	theme	drug	1255:1258	arg1	system					1269:1274	supersaturated SLB self-nanoemulsion drug delivery system	1218:1274	supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS)	1218:1287	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	54	theme	drug	1255:1258	arg1	SLB-SSNEDDS					1276:1286	SLB-SSNEDDS	1276:1286	SLB-SSNEDDS	1276:1286	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	8	55	theme	other	1965:1969	arg1	hand					1971:1974	the other hand	1961:1974	the other hand	1961:1974	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	6	56	theme	%	1675:1675	arg1	bioavailability					1636:1650	a relative bioavailability	1625:1650	a relative bioavailability of 578.45% and 1 139.44% respectively	1625:1688	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	5	57	theme	delivery	1320:1327	arg1	SLB-SNEDDS					1336:1345	SLB-SNEDDS	1336:1345	SLB-SNEDDS	1336:1345	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	57	theme	delivery	1320:1327	arg1	system					1329:1334	SLB self-nanoemulsion drug delivery system	1293:1334	SLB self-nanoemulsion drug delivery system(SLB-SNEDDS)	1293:1346	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	1	58	theme	self-nanoemulsifying	247:266	arg1	system					282:287	supersaturable self-nanoemulsifying drug delivery system	232:287	supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	232:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	1	59	theme	succinate	176:184	arg1	effects					127:133	the effects	123:133	the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	123:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	8	60	theme	osmotic	1981:1987	arg1	process					1999:2005	the osmotic transport process	1977:2005	the osmotic transport process of SLB	1977:2012	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	6	61	with	times	1553:1557	arg1	bioavailability					1636:1650	a relative bioavailability	1625:1650	a relative bioavailability of 578.45% and 1 139.44% respectively	1625:1688	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	4	62	theme	Caco-2	897:902	arg1	cells					904:908	Caco-2 cells	897:908	Caco-2 cells viability	897:918	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	6	63	theme	pharmacokinetic	1397:1411	arg1	parameters					1413:1422	pharmacokinetic parameters	1397:1422	pharmacokinetic parameters	1397:1422	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	1	64	theme	HPMCAS	186:191	arg1	succinate					176:184	Hydroxypropyl methylcellulose acetate succinate	138:184	Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF)	138:195	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	1	64	theme	HPMCAS	186:191	arg1	MF					193:194	HPMCAS MF	186:194	HPMCAS MF	186:194	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	5	65	theme	self-nanoemulsion	1297:1313	arg1	SLB-SNEDDS					1336:1345	SLB-SNEDDS	1336:1345	SLB-SNEDDS	1336:1345	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	65	theme	self-nanoemulsion	1297:1313	arg1	system					1329:1334	SLB self-nanoemulsion drug delivery system	1293:1334	SLB self-nanoemulsion drug delivery system(SLB-SNEDDS)	1293:1346	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	3	66	dep	in	611:612	arg1	vitro					614:618	vitro	614:618	vitro	614:618	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	8	67	theme	SLB	2187:2189	arg1	bioavailability					2168:2182	the oral bioavailability	2159:2182	the oral bioavailability of SLB	2159:2189	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	8	68	theme	SLB	2010:2012	arg1	process					1999:2005	the osmotic transport process	1977:2005	the osmotic transport process of SLB	1977:2012	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	5	69	from	difference	1171:1180	arg1	transport					1200:1208	supersaturated transport	1185:1208	supersaturated transport	1185:1208	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	6	70	theme	SLB	1598:1600	arg1	group					1613:1617	SLB suspension group	1598:1617	SLB suspension group	1598:1617	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	5	71	contain	had	1047:1049	arg2	increase					1070:1077	a very significant increase	1051:1077	a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min	1051:1146	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	71	contain	had	1047:1049	arg1	app					999:1001	app	999:1001	app	999:1001	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	71	contain	had	1047:1049	arg1	AP→BL					1004:1008	AP→BL	1004:1008	AP→BL	1004:1008	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	71	contain	had	1047:1049	arg1	P_					996:997	the P_	992:997	the P_(app)(AP→BL) of the self-emulsifying preparation	992:1045	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	4	72	theme	MTT	713:715	arg1	results					702:708	The results	698:708	The results of MTT	698:715	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	3	73	theme	supersaturation	620:634	arg1	correlation					636:646	The in vivo and in vitro supersaturation correlation	595:646	The in vivo and in vitro supersaturation correlation	595:646	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	2	74	theme	cell	573:576	arg1	model					588:592	a Caco-2 cell monolayer model	564:592	a Caco-2 cell monolayer model	564:592	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	0	75	from	system	102:107	arg1	absorption					25:34	absorption	25:34	absorption of silybin from supersaturable self-nanoemulsifying drug delivery system	25:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	0	76	theme	supersaturable	52:65	arg1	system					102:107	supersaturable self-nanoemulsifying drug delivery system	52:107	supersaturable self-nanoemulsifying drug delivery system	52:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	3	77	theme	in	611:612	arg1	correlation					636:646	The in vivo and in vitro supersaturation correlation	595:646	The in vivo and in vitro supersaturation correlation	595:646	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	5	78	dep	increase	1070:1077	arg1	reduced					1117:1123	reduced	1117:1123	can be reduced by polymer in 0-30 min	1110:1146	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	8	79	theme	preparations	2059:2070	arg1	composition					2040:2050	the composition	2036:2050	the composition of its preparations	2036:2070	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	1	80	theme	silybin	214:220	arg1	absorption					200:209	absorption	200:209	absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	200:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	0	81	theme	drug	88:91	arg1	system					102:107	supersaturable self-nanoemulsifying drug delivery system	52:107	supersaturable self-nanoemulsifying drug delivery system	52:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	1	82	theme	methylcellulose	152:166	arg1	succinate					176:184	Hydroxypropyl methylcellulose acetate succinate	138:184	Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF)	138:195	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	1	82	theme	methylcellulose	152:166	arg1	MF					193:194	HPMCAS MF	186:194	HPMCAS MF	186:194	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	2	83	theme	promoting	480:488	arg1	effect					490:495	absorption promoting effect	469:495	absorption promoting effect of the self-emulsifying preparation	469:531	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	6	84	theme	suspension	1602:1611	arg1	group					1613:1617	SLB suspension group	1598:1617	SLB suspension group	1598:1617	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	4	85	theme	polymer	860:866	arg1	addition					848:855	the addition	844:855	the addition of polymer	844:866	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	8	86	dep	transport	2116:2124	arg1	the					2112:2114	the	2112:2114	the	2112:2114	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	2	87	theme	self-emulsifying	504:519	arg1	preparation					521:531	the self-emulsifying preparation	500:531	the self-emulsifying preparation	500:531	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	4	88	theme	significant	875:885	arg1	effect					887:892	no significant effect	872:892	no significant effect	872:892	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	1	89	from	effects	127:133	arg1	absorption					200:209	absorption	200:209	absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment	200:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	8	90	from	solubility	1859:1868	arg1	tract					1897:1901	gastrointestinal tract	1880:1901	gastrointestinal tract	1880:1901	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	2	91	theme	in	434:435	arg1	permeability					452:463	the in vitro membrane permeability	430:463	the in vitro membrane permeability	430:463	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	2	92	theme	preparation	381:391	arg1	toxicity					352:359	The cell toxicity	343:359	The cell toxicity of self-emulsifying preparation	343:391	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	0	93	theme	MF	19:20	arg1	[Effects					0:7	[Effects	0:7	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system	0:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	5	94	theme	significant	1058:1068	arg1	increase					1070:1077	a very significant increase	1051:1077	a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min	1051:1146	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	6	95	theme	groups	1495:1500	arg1	concentration					1446:1458	the blood concentration	1436:1458	the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups	1436:1500	As compared with SLB suspension, pharmacokinetic parameters showed that the blood concentration of both SLB-SNEDDS and SLB-SSNEDDS groups were significantly increased, and C_(max) was 5.25 times and 9.69 times respectively of that in SLB suspension group, with a relative bioavailability of 578.45% and 1 139.44% respectively.					
33787105	1	96	dep	stage	325:329	arg1	experiment					331:340	experiment	331:340	the early stage experiment	315:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	5	97	theme	delivery	1260:1267	arg1	system					1269:1274	supersaturated SLB self-nanoemulsion drug delivery system	1218:1274	supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS)	1218:1287	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	97	theme	delivery	1260:1267	arg1	SLB-SSNEDDS					1276:1286	SLB-SSNEDDS	1276:1286	SLB-SSNEDDS	1276:1286	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	0	98	theme	silybin	39:45	arg1	absorption					25:34	absorption	25:34	absorption of silybin from supersaturable self-nanoemulsifying drug delivery system	25:107	[Effects of HPMCAS MF on absorption of silybin from supersaturable self-nanoemulsifying drug delivery system].					
33787105	5	99	theme	preparation	1035:1045	arg1	app					999:1001	app	999:1001	app	999:1001	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	99	theme	preparation	1035:1045	arg1	AP→BL					1004:1008	AP→BL	1004:1008	AP→BL	1004:1008	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	99	theme	preparation	1035:1045	arg1	P_					996:997	the P_	992:997	the P_(app)(AP→BL) of the self-emulsifying preparation	992:1045	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	7	100	theme	relative	1703:1710	arg1	bioavailability					1712:1726	relative bioavailability	1703:1726	relative bioavailability of SLB-SSNEDDS	1703:1741	C_(max) and relative bioavailability of SLB-SSNEDDS were 1.85 times and 197% of those of SLB-SNEDDS, respectively.					
33787105	5	101	theme	solution	942:949	arg1	group					951:955	the solution group	938:955	the solution group	938:955	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	8	102	theme	supersaturation	1935:1949	arg1	state					1951:1955	SLB supersaturation state	1931:1955	SLB supersaturation state	1931:1955	Therefore, on the one hand, SSNEDDS can increase the solubility of SLB in gastrointestinal tract by maintaining stability of SLB supersaturation state; on the other hand, the osmotic transport process of SLB was regulated through the composition of its preparations, and both of them could jointly promote the transport and absorption of SLB to improve the oral bioavailability of SLB.					
33787105	2	103	theme	membrane	443:450	arg1	permeability					452:463	the in vitro membrane permeability	430:463	the in vitro membrane permeability	430:463	The cell toxicity of self-emulsifying preparation was evaluated by the MTT method, and the in vitro membrane permeability and absorption promoting effect of the self-emulsifying preparation were evaluated by establishing a Caco-2 cell monolayer model.					
33787105	1	104	theme	early	319:323	arg1	stage					325:329	the early stage experiment	315:340	the early stage experiment	315:340	To evaluate the effects of Hydroxypropyl methylcellulose acetate succinate(HPMCAS MF) on absorption of silybin(SLB) from supersaturable self-nanoemulsifying drug delivery system which was pre-prepared at the early stage experiment.					
33787105	4	105	contain	had	868:870	arg1	addition					848:855	the addition	844:855	the addition of polymer	844:866	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	4	105	contain	had	868:870	arg2	effect					887:892	no significant effect	872:892	no significant effect	872:892	The results of MTT showed that the concentration of the preparation below 2 mg·mL~(-1)(C_(SLB) 100 μg·mL~(-1)) was not toxic to Caco-2 cells, and the addition of polymer had no significant effect on Caco-2 cells viability.					
33787105	3	106	theme	blood	670:674	arg1	concentration					676:688	the blood concentration	666:688	the blood concentration of SLB	666:695	The in vivo and in vitro supersaturation correlation was evaluated via the blood concentration of SLB.					
33787105	5	107	theme	supersaturated	1218:1231	arg1	system					1269:1274	supersaturated SLB self-nanoemulsion drug delivery system	1218:1274	supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS)	1218:1287	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	107	theme	supersaturated	1218:1231	arg1	SLB-SSNEDDS					1276:1286	SLB-SSNEDDS	1276:1286	SLB-SSNEDDS	1276:1286	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	108	theme	self-nanoemulsion	1237:1253	arg1	system					1269:1274	supersaturated SLB self-nanoemulsion drug delivery system	1218:1274	supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS)	1218:1287	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
33787105	5	108	theme	self-nanoemulsion	1237:1253	arg1	SLB-SSNEDDS					1276:1286	SLB-SSNEDDS	1276:1286	SLB-SSNEDDS	1276:1286	As compared with the solution group, the transport results showed that the P_(app)(AP→BL) of the self-emulsifying preparation had a very significant increase; the transport rate of silybin can be reduced by polymer in 0-30 min; however, there was no difference in supersaturated transport between supersaturated SLB self-nanoemulsion drug delivery system(SLB-SSNEDDS) and SLB self-nanoemulsion drug delivery system(SLB-SNEDDS) within 2 hours.					
32172898	7	0	theme	healing	1062:1068	arg1	process					1070:1076	the wound healing process	1052:1076	the wound healing process without scars in the mouse experiment	1052:1114	Moreover, the hydrogel can inhibit bacterial infection and accelerate the wound healing process without scars in the mouse experiment.					
32172898	1	1	theme	wound	192:196	arg1	hydrogels					157:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	1	1	theme	wound	192:196	arg1	dressings					198:206	wound dressings	192:206	wound dressings because of their biocompatibility and ability	192:252	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	5	2	theme	mechanical	712:721	arg1	properties					723:732	mechanical properties	712:732	mechanical properties of the hydrogel	712:748	The TA not only acted as a crosslinker to enhance mechanical properties of the hydrogel, but also as an antibacterial agent which could be sustainably released in response to the acidic environment.					
32172898	5	3	theme	acidic	841:846	arg1	environment					848:858	the acidic environment	837:858	the acidic environment	837:858	The TA not only acted as a crosslinker to enhance mechanical properties of the hydrogel, but also as an antibacterial agent which could be sustainably released in response to the acidic environment.					
32172898	4	4	theme	physiological	635:647	arg1	temperature					649:659	physiological temperature	635:659	physiological temperature	635:659	The pre-cooled hydrogel precursor solution can be injected onto the irregular wound area and gel rapidly at physiological temperature.					
32172898	3	5	theme	pH-sensitive	401:412	arg1	hydroxypropyl					414:426	a novel thermosensitive and pH-sensitive hydroxypropyl	373:426	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	6	6	theme	composite	865:873	arg1	hydrogel					875:882	The composite hydrogel	861:882	The composite hydrogel	861:882	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	3	7	theme	hydroxypropyl	414:426	arg1	hydrogel					481:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	4	8	theme	hydrogel	542:549	arg1	solution					561:568	The pre-cooled hydrogel precursor solution	527:568	The pre-cooled hydrogel precursor solution	527:568	The pre-cooled hydrogel precursor solution can be injected onto the irregular wound area and gel rapidly at physiological temperature.					
32172898	3	9	theme	thermosensitive	381:395	arg1	hydroxypropyl					414:426	a novel thermosensitive and pH-sensitive hydroxypropyl	373:426	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	4	10	theme	pre-cooled	531:540	arg1	solution					561:568	The pre-cooled hydrogel precursor solution	527:568	The pre-cooled hydrogel precursor solution	527:568	The pre-cooled hydrogel precursor solution can be injected onto the irregular wound area and gel rapidly at physiological temperature.					
32172898	7	11	from	process	1070:1076	arg1	experiment					1105:1114	the mouse experiment	1095:1114	the mouse experiment	1095:1114	Moreover, the hydrogel can inhibit bacterial infection and accelerate the wound healing process without scars in the mouse experiment.					
32172898	0	12	theme	chitin	66:71	arg1	hydrogel					73:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel	0:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.	0:140	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	1	13	used	used	184:187	arg2	dressings					198:206	wound dressings	192:206	wound dressings because of their biocompatibility and ability	192:252	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	1	13	used	used	184:187	arg2	hydrogels					157:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	3	14	theme	ion	454:456	arg1	hydrogel					481:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	4	15	theme	precursor	551:559	arg1	solution					561:568	The pre-cooled hydrogel precursor solution	527:568	The pre-cooled hydrogel precursor solution	527:568	The pre-cooled hydrogel precursor solution can be injected onto the irregular wound area and gel rapidly at physiological temperature.					
32172898	7	16	theme	bacterial	1017:1025	arg1	infection					1027:1035	bacterial infection	1017:1035	bacterial infection	1017:1035	Moreover, the hydrogel can inhibit bacterial infection and accelerate the wound healing process without scars in the mouse experiment.					
32172898	6	17	theme	negligible	957:966	arg1	cytotoxicity					968:979	negligible cytotoxicity	957:979	negligible cytotoxicity	957:979	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	3	18	theme	acid/ferric	442:452	arg1	hydrogel					481:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	0	19	theme	Thermosensitive	0:14	arg1	hydrogel					73:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel	0:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.	0:140	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	8	20	theme	potential	1144:1152	arg1	application					1154:1164	the potential application	1140:1164	the potential application of this composite hydrogel for the infected wound healing	1140:1222	These results indicate the potential application of this composite hydrogel for the infected wound healing.					
32172898	1	21	theme	biocompatibility	225:240	arg1	hydrogels					157:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	1	21	theme	biocompatibility	225:240	arg1	dressings					198:206	wound dressings	192:206	wound dressings because of their biocompatibility and ability	192:252	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	0	22	theme	tannin-containing	34:50	arg1	hydrogel					73:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel	0:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.	0:140	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	2	23	theme	healing	348:354	arg1	process					356:362	the healing process	344:362	the healing process	344:362	However, bacterial infection often delays the healing process.					
32172898	0	24	theme	pH-responsive	20:32	arg1	hydrogel					73:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel	0:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.	0:140	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	1	25	theme	Polysaccharide	142:155	arg1	hydrogels					157:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	1	25	theme	Polysaccharide	142:155	arg1	dressings					198:206	wound dressings	192:206	wound dressings because of their biocompatibility and ability	192:252	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	1	26	theme	ability	246:252	arg1	hydrogels					157:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels	142:165	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	1	26	theme	ability	246:252	arg1	dressings					198:206	wound dressings	192:206	wound dressings because of their biocompatibility and ability	192:252	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	3	27	theme	composite	471:479	arg1	hydrogel					481:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	8	28	theme	hydrogel	1184:1191	arg1	application					1154:1164	the potential application	1140:1164	the potential application of this composite hydrogel for the infected wound healing	1140:1222	These results indicate the potential application of this composite hydrogel for the infected wound healing.					
32172898	3	29	theme	simple	510:515	arg1	assembly					517:524	a simple assembly	508:524	a simple assembly	508:524	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	0	30	theme	hydroxypropyl	52:64	arg1	hydrogel					73:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel	0:80	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.	0:140	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	4	31	theme	irregular	595:603	arg1	area					611:614	the irregular wound area	591:614	the irregular wound area	591:614	The pre-cooled hydrogel precursor solution can be injected onto the irregular wound area and gel rapidly at physiological temperature.					
32172898	5	32	theme	antibacterial	766:778	arg1	agent					780:784	an antibacterial agent	763:784	an antibacterial agent which could be sustainably released in response to the acidic environment	763:858	The TA not only acted as a crosslinker to enhance mechanical properties of the hydrogel, but also as an antibacterial agent which could be sustainably released in response to the acidic environment.					
32172898	3	33	theme	chitin/tannic	428:440	arg1	hydrogel					481:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	3	34	theme	HPCH/TA/Fe	459:468	arg1	hydrogel					481:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	3	35	theme	novel	375:379	arg1	hydroxypropyl					414:426	a novel thermosensitive and pH-sensitive hydroxypropyl	373:426	a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel	373:488	Herein, a novel thermosensitive and pH-sensitive hydroxypropyl chitin/tannic acid/ferric ion (HPCH/TA/Fe) composite hydrogel was fabricated by a simple assembly.					
32172898	7	36	theme	mouse	1099:1103	arg1	experiment					1105:1114	the mouse experiment	1095:1114	the mouse experiment	1095:1114	Moreover, the hydrogel can inhibit bacterial infection and accelerate the wound healing process without scars in the mouse experiment.					
32172898	8	37	theme	composite	1174:1182	arg1	hydrogel					1184:1191	this composite hydrogel	1169:1191	this composite hydrogel	1169:1191	These results indicate the potential application of this composite hydrogel for the infected wound healing.					
32172898	5	38	theme	hydrogel	741:748	arg1	properties					723:732	mechanical properties	712:732	mechanical properties of the hydrogel	712:748	The TA not only acted as a crosslinker to enhance mechanical properties of the hydrogel, but also as an antibacterial agent which could be sustainably released in response to the acidic environment.					
32172898	6	39	theme	antibacterial	916:928	arg1	activity					930:937	excellent broad-spectrum antibacterial activity	891:937	excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity	891:979	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	4	40	theme	wound	605:609	arg1	area					611:614	the irregular wound area	591:614	the irregular wound area	591:614	The pre-cooled hydrogel precursor solution can be injected onto the irregular wound area and gel rapidly at physiological temperature.					
32172898	8	41	theme	wound	1210:1214	arg1	healing					1216:1222	the infected wound healing	1197:1222	the infected wound healing	1197:1222	These results indicate the potential application of this composite hydrogel for the infected wound healing.					
32172898	6	42	theme	broad-spectrum	901:914	arg1	activity					930:937	excellent broad-spectrum antibacterial activity	891:937	excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity	891:979	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	8	43	theme	infected	1201:1208	arg1	healing					1216:1222	the infected wound healing	1197:1222	the infected wound healing	1197:1222	These results indicate the potential application of this composite hydrogel for the infected wound healing.					
32172898	1	44	theme	moist	265:269	arg1	environment					271:281	moist environment	265:281	moist environment	265:281	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	2	45	theme	bacterial	311:319	arg1	infection					321:329	bacterial infection	311:329	bacterial infection	311:329	However, bacterial infection often delays the healing process.					
32172898	6	46	theme	excellent	891:899	arg1	activity					930:937	excellent broad-spectrum antibacterial activity	891:937	excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity	891:979	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	6	47	dep	days	947:950	arg1	up					939:940	up	939:940	up	939:940	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	0	48	theme	antibacterial	100:112	arg1	activity					114:121	long-lasting antibacterial activity	87:121	long-lasting antibacterial activity	87:121	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	0	49	with	hydrogel	73:80	arg1	activity					114:121	long-lasting antibacterial activity	87:121	long-lasting antibacterial activity	87:121	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	0	50	theme	long-lasting	87:98	arg1	activity					114:121	long-lasting antibacterial activity	87:121	long-lasting antibacterial activity	87:121	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32172898	1	51	theme	wound	287:291	arg1	healing					293:299	wound healing	287:299	wound healing	287:299	Polysaccharide hydrogels have been widely used as wound dressings because of their biocompatibility and ability to provide moist environment for wound healing.					
32172898	7	52	theme	wound	1056:1060	arg1	healing					1062:1068	wound healing	1056:1068	the wound healing process without scars in the mouse experiment	1052:1114	Moreover, the hydrogel can inhibit bacterial infection and accelerate the wound healing process without scars in the mouse experiment.					
32172898	6	53	with	days	947:950	arg1	cytotoxicity					968:979	negligible cytotoxicity	957:979	negligible cytotoxicity	957:979	The composite hydrogel showed excellent broad-spectrum antibacterial activity up to 7 days with negligible cytotoxicity.					
32172898	0	54	theme	wound	127:131	arg1	healing					133:139	wound healing	127:139	wound healing	127:139	Thermosensitive and pH-responsive tannin-containing hydroxypropyl chitin hydrogel with long-lasting antibacterial activity for wound healing.					
32229122	6	0	theme	correlation	993:1003	arg1	analysis					1005:1012	Canonical correlation analysis	983:1012	Canonical correlation analysis	983:1012	Canonical correlation analysis allowed us to explore the relationships between both sets of variables.					
32229122	0	1	from	sheep	141:145	arg1	composition					72:82	composition	72:82	composition	72:82	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	1	from	sheep	141:145	arg1	quality					94:100	hygienic quality	85:100	hygienic quality	85:100	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	1	from	sheep	141:145	arg1	color					65:69	color	65:69	color	65:69	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	1	from	sheep	141:145	arg1	milk					122:125	milk	122:125	milk from Manchega sheep	122:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	1	from	sheep	141:145	arg1	coagulation					107:117	coagulation	107:117	coagulation of milk from Manchega sheep	107:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	7	2	theme	canonical	1142:1150	arg1	values					1152:1157	canonical values	1142:1157	canonical values	1142:1157	Canonical correlations for the first and second pair of canonical values were 0.794 and 0.438, respectively.					
32229122	9	3	theme	strong	1333:1338	arg1	correlation					1340:1350	a strong correlation	1331:1350	a strong correlation with fat and protein content and with total solids	1331:1401	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	6	4	theme	variables	1075:1083	arg1	variables					1075:1083	variables	1075:1083	variables	1075:1083	Canonical correlation analysis allowed us to explore the relationships between both sets of variables.					
32229122	6	4	theme	variables	1075:1083	arg1	sets					1067:1070	both sets	1062:1070	both sets of variables	1062:1083	Canonical correlation analysis allowed us to explore the relationships between both sets of variables.					
32229122	9	5	contain	had	1327:1329	arg2	correlation					1340:1350	a strong correlation	1331:1350	a strong correlation with fat and protein content and with total solids	1331:1401	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	9	5	contain	had	1327:1329	arg1	values					1320:1325	color values	1314:1325	color values	1314:1325	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	11	6	theme	color	1666:1670	arg1	values					1672:1677	color values	1666:1677	color values	1666:1677	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	7	7	theme	values	1152:1157	arg1	pair					1134:1137	the first and second pair	1113:1137	the first and second pair of canonical values	1113:1157	Canonical correlations for the first and second pair of canonical values were 0.794 and 0.438, respectively.					
32229122	0	8	from	composition	72:82	arg1	sheep					141:145	Manchega sheep	132:145	Manchega sheep	132:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	10	9	theme	standardized	1509:1520	arg1	variability					1532:1542	standardized canonical variability	1509:1542	standardized canonical variability	1509:1542	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	0	10	theme	milk	122:125	arg1	composition					72:82	composition	72:82	composition	72:82	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	10	theme	milk	122:125	arg1	quality					94:100	hygienic quality	85:100	hygienic quality	85:100	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	10	theme	milk	122:125	arg1	color					65:69	color	65:69	color	65:69	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	0	10	theme	milk	122:125	arg1	coagulation					107:117	coagulation	107:117	coagulation of milk from Manchega sheep	107:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	1	11	theme	coagulation	352:362	arg1	quality					324:330	the quality	320:330	the quality of raw milk and its coagulation	320:362	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	1	12	theme	hygienic	207:214	arg1	quality					216:222	hygienic quality	207:222	hygienic quality	207:222	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	0	13	from	color	65:69	arg1	sheep					141:145	Manchega sheep	132:145	Manchega sheep	132:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	2	14	theme	Spain	509:513	arg1	region					479:484	the region	475:484	the region of Castilla-La Mancha, Spain	475:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	0	15	theme	Manchega	132:139	arg1	sheep					141:145	Manchega sheep	132:145	Manchega sheep	132:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	4	16	theme	a	835:835	arg1	value					828:832	red/green value	818:832	red/green value (a*)	818:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	16	theme	a	835:835	arg1	*					836:836	a*	835:836	a*	835:836	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	5	17	theme	milk	944:947	arg1	composition					949:959	milk composition	944:959	milk composition	944:959	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	1	18	theme	valid	301:305	arg1	colorimetry					270:280	colorimetry	270:280	colorimetry	270:280	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	1	18	theme	valid	301:305	arg1	predictor					307:315	a valid predictor	299:315	a valid predictor of the quality of raw milk and its coagulation	299:362	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	2	19	theme	sheep	445:449	arg1	milk					451:454	Manchega sheep milk	436:454	Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	436:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	7	20	theme	second	1127:1132	arg1	pair					1134:1137	the first and second pair	1113:1137	the first and second pair of canonical values	1113:1157	Canonical correlations for the first and second pair of canonical values were 0.794 and 0.438, respectively.					
32229122	9	21	theme	weak	1419:1422	arg1	correlation					1424:1434	a weak correlation	1417:1434	a weak correlation with lactose content and somatic cell score	1417:1478	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	4	22	theme	lactose	737:743	arg1	variables					687:695	The variables	683:695	The variables with a higher discriminant ability	683:730	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	22	theme	lactose	737:743	arg1	content					745:751	lactose content	737:751	lactose content	737:751	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	0	23	theme	hygienic	85:92	arg1	quality					94:100	hygienic quality	85:100	hygienic quality	85:100	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	1	24	theme	quality	324:330	arg1	colorimetry					270:280	colorimetry	270:280	colorimetry	270:280	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	1	24	theme	quality	324:330	arg1	predictor					307:315	a valid predictor	299:315	a valid predictor of the quality of raw milk and its coagulation	299:362	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	8	25	theme	observed	1254:1261	arg1	variability					1263:1273	the observed variability	1250:1273	the observed variability	1250:1273	Both values were significant and represented 92.82% of the observed variability.					
32229122	1	26	theme	raw	335:337	arg1	milk					339:342	raw milk	335:342	raw milk	335:342	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	5	27	theme	predictive	881:890	arg1	ability					892:898	a predictive ability	879:898	a predictive ability similar to that found in the model based on milk composition and hygienic quality	879:980	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	2	28	from	samples	425:431	arg1	flocks					463:468	4 flocks	461:468	4 flocks	461:468	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	2	28	from	samples	425:431	arg1	region					479:484	the region	475:484	the region of Castilla-La Mancha, Spain	475:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	4	29	theme	higher	704:709	arg1	ability					724:730	a higher discriminant ability	702:730	a higher discriminant ability	702:730	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	11	30	theme	coagulation	1772:1782	arg1	ability					1784:1790	the coagulation ability	1768:1790	the coagulation ability of Manchega sheep milk	1768:1813	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	7	31	theme	Canonical	1086:1094	arg1	correlations					1096:1107	Canonical correlations	1086:1107	Canonical correlations for the first and second pair of canonical values	1086:1157	Canonical correlations for the first and second pair of canonical values were 0.794 and 0.438, respectively.					
32229122	9	32	theme	somatic	1461:1467	arg1	score					1474:1478	somatic cell score	1461:1478	somatic cell score	1461:1478	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	5	33	theme	color	859:863	arg1	values					865:870	color values	859:870	color values	859:870	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	11	34	theme	sheep	1804:1808	arg1	milk					1810:1813	Manchega sheep milk	1795:1813	Manchega sheep milk	1795:1813	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	10	35	theme	predictable	1566:1576	arg1	measure					1578:1584	a predictable measure	1564:1584	a predictable measure of the composition	1564:1603	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	2	36	theme	individual	414:423	arg1	samples					425:431	1,200 individual samples	408:431	1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	408:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	9	37	with	correlation	1340:1350	arg1	fat					1357:1359	fat	1357:1359	fat	1357:1359	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	9	37	with	correlation	1340:1350	arg1	solids					1396:1401	total solids	1390:1401	total solids	1390:1401	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	4	38	theme	cell	762:765	arg1	score					767:771	somatic cell score	754:771	somatic cell score	754:771	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	10	39	theme	composition	1593:1603	arg1	measure					1578:1584	a predictable measure	1564:1584	a predictable measure of the composition	1564:1603	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	3	40	dep	differences	567:577	arg1	the					563:565	the	563:565	the	563:565	Based on the measured variables, we determined the differences and similarities between coagulating and noncoagulating samples using discriminant analysis techniques.					
32229122	5	41	theme	similar	900:906	arg1	ability					892:898	a predictive ability	879:898	a predictive ability similar to that found in the model based on milk composition and hygienic quality	879:980	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	2	42	from	region	479:484	arg1	milk					451:454	Manchega sheep milk	436:454	Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	436:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	2	42	from	region	479:484	arg1	samples					425:431	1,200 individual samples	408:431	1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	408:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	0	43	from	coagulation	107:117	arg1	sheep					141:145	Manchega sheep	132:145	Manchega sheep	132:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	4	44	with	variables	687:695	arg1	ability					724:730	a higher discriminant ability	702:730	a higher discriminant ability	702:730	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	3	45	dep	coagulating	604:614	arg1	samples					635:641	samples	635:641	samples	635:641	Based on the measured variables, we determined the differences and similarities between coagulating and noncoagulating samples using discriminant analysis techniques.					
32229122	1	46	used	used	291:294	arg2	predictor					307:315	a valid predictor	299:315	a valid predictor of the quality of raw milk and its coagulation	299:362	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	1	46	used	used	291:294	arg2	colorimetry					270:280	colorimetry	270:280	colorimetry	270:280	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	4	47	theme	L	810:810	arg1	lightness					799:807	lightness	799:807	lightness (L*)	799:812	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	47	theme	L	810:810	arg1	*					811:811	L*	810:811	L*	810:811	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	6	48	theme	Canonical	983:991	arg1	analysis					1005:1012	Canonical correlation analysis	983:1012	Canonical correlation analysis	983:1012	Canonical correlation analysis allowed us to explore the relationships between both sets of variables.					
32229122	3	49	theme	discriminant	649:660	arg1	techniques					671:680	discriminant analysis techniques	649:680	discriminant analysis techniques	649:680	Based on the measured variables, we determined the differences and similarities between coagulating and noncoagulating samples using discriminant analysis techniques.					
32229122	1	50	theme	milk	245:248	arg1	composition					194:204	composition	194:204	composition	194:204	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	1	50	theme	milk	245:248	arg1	quality					216:222	hygienic quality	207:222	hygienic quality	207:222	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	1	50	theme	milk	245:248	arg1	values					235:240	color values	229:240	color values of milk	229:248	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	4	51	theme	color	786:790	arg1	lightness					799:807	lightness	799:807	lightness (L*)	799:812	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	51	theme	color	786:790	arg1	values					792:797	the color values lightness (L*) and red/green value (a*)	782:837	the color values lightness (L*) and red/green value (a*)	782:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	51	theme	color	786:790	arg1	value					828:832	red/green value	818:832	red/green value (a*)	818:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	10	52	theme	first	1487:1491	arg1	combinations					1493:1504	The 2 first combinations	1481:1504	The 2 first combinations of standardized canonical variability	1481:1542	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	9	53	theme	protein	1365:1371	arg1	content					1373:1379	protein content	1365:1379	protein content	1365:1379	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	10	54	theme	canonical	1522:1530	arg1	variability					1532:1542	standardized canonical variability	1509:1542	standardized canonical variability	1509:1542	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	11	55	theme	values	1672:1677	arg1	means					1710:1714	a rapid and effective means	1688:1714	a rapid and effective means of supplementing standard analyses	1688:1749	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	11	55	theme	values	1672:1677	arg1	Measurement					1651:1661	Measurement	1651:1661	Measurement of color values	1651:1677	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	5	56	theme	hygienic	965:972	arg1	quality					974:980	hygienic quality	965:980	hygienic quality	965:980	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	3	57	theme	measured	529:536	arg1	variables					538:546	the measured variables	525:546	the measured variables	525:546	Based on the measured variables, we determined the differences and similarities between coagulating and noncoagulating samples using discriminant analysis techniques.					
32229122	9	58	with	correlation	1424:1434	arg1	content					1449:1455	lactose content	1441:1455	lactose content	1441:1455	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	9	58	with	correlation	1424:1434	arg1	score					1474:1478	somatic cell score	1461:1478	somatic cell score	1461:1478	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	11	59	theme	effective	1700:1708	arg1	means					1710:1714	a rapid and effective means	1688:1714	a rapid and effective means of supplementing standard analyses	1688:1749	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	11	59	theme	effective	1700:1708	arg1	Measurement					1651:1661	Measurement	1651:1661	Measurement of color values	1651:1677	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	9	60	contain	had	1413:1415	arg2	correlation					1424:1434	a weak correlation	1417:1434	a weak correlation with lactose content and somatic cell score	1417:1478	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	9	60	contain	had	1413:1415	arg1	they					1408:1411	they	1408:1411	they	1408:1411	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	9	61	theme	total	1390:1394	arg1	solids					1396:1401	total solids	1390:1401	total solids	1390:1401	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	4	62	theme	red/green	818:826	arg1	value					828:832	red/green value	818:832	red/green value (a*)	818:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	62	theme	red/green	818:826	arg1	*					836:836	a*	835:836	a*	835:836	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	62	theme	red/green	818:826	arg1	values					792:797	the color values lightness (L*) and red/green value (a*)	782:837	the color values lightness (L*) and red/green value (a*)	782:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	2	63	from	flocks	463:468	arg1	milk					451:454	Manchega sheep milk	436:454	Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	436:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	2	63	from	flocks	463:468	arg1	samples					425:431	1,200 individual samples	408:431	1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	408:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	11	64	theme	rapid	1690:1694	arg1	means					1710:1714	a rapid and effective means	1688:1714	a rapid and effective means of supplementing standard analyses	1688:1749	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	11	64	theme	rapid	1690:1694	arg1	Measurement					1651:1661	Measurement	1651:1661	Measurement of color values	1651:1677	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	0	65	from	quality	94:100	arg1	sheep					141:145	Manchega sheep	132:145	Manchega sheep	132:145	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	2	66	theme	milk	451:454	arg1	samples					425:431	1,200 individual samples	408:431	1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	408:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	7	67	theme	first	1117:1121	arg1	pair					1134:1137	the first and second pair	1113:1137	the first and second pair of canonical values	1113:1157	Canonical correlations for the first and second pair of canonical values were 0.794 and 0.438, respectively.					
32229122	2	68	theme	Manchega	436:443	arg1	milk					451:454	Manchega sheep milk	436:454	Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain	436:513	For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain.					
32229122	10	69	theme	milk	1645:1648	arg1	hygiene					1634:1640	the hygiene	1630:1640	the hygiene of milk	1630:1648	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	9	70	theme	lactose	1441:1447	arg1	content					1449:1455	lactose content	1441:1455	lactose content	1441:1455	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	11	71	theme	Manchega	1795:1802	arg1	milk					1810:1813	Manchega sheep milk	1795:1813	Manchega sheep milk	1795:1813	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	0	72	theme	multivariate	6:17	arg1	analysis					19:26	multivariate analysis	6:26	multivariate analysis	6:26	Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.					
32229122	1	73	theme	milk	339:342	arg1	quality					324:330	the quality	320:330	the quality of raw milk and its coagulation	320:362	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	8	74	theme	variability	1263:1273	arg1	%					1245:1245	92.82%	1240:1245	92.82% of the observed variability	1240:1273	Both values were significant and represented 92.82% of the observed variability.					
32229122	8	74	theme	variability	1263:1273	arg1	variability					1263:1273	the observed variability	1250:1273	the observed variability	1250:1273	Both values were significant and represented 92.82% of the observed variability.					
32229122	10	75	theme	variability	1532:1542	arg1	combinations					1493:1504	The 2 first combinations	1481:1504	The 2 first combinations of standardized canonical variability	1481:1542	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	4	76	dep	values	792:797	arg1	lightness					799:807	lightness	799:807	lightness (L*)	799:812	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	76	dep	values	792:797	arg1	*					811:811	L*	810:811	L*	810:811	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	76	dep	values	792:797	arg1	value					828:832	red/green value	818:832	red/green value (a*)	818:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	76	dep	values	792:797	arg1	*					836:836	a*	835:836	a*	835:836	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	4	76	dep	values	792:797	arg1	values					792:797	the color values lightness (L*) and red/green value (a*)	782:837	the color values lightness (L*) and red/green value (a*)	782:837	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	9	77	theme	cell	1469:1472	arg1	score					1474:1478	somatic cell score	1461:1478	somatic cell score	1461:1478	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	4	78	theme	discriminant	711:722	arg1	ability					724:730	a higher discriminant ability	702:730	a higher discriminant ability	702:730	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	11	79	theme	milk	1810:1813	arg1	ability					1784:1790	the coagulation ability	1768:1790	the coagulation ability of Manchega sheep milk	1768:1813	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	11	80	theme	standard	1733:1740	arg1	analyses					1742:1749	standard analyses	1733:1749	standard analyses	1733:1749	Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.					
32229122	9	81	theme	correlation	1280:1290	arg1	structure					1292:1300	The correlation structure	1276:1300	The correlation structure	1276:1300	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
32229122	4	82	theme	somatic	754:760	arg1	score					767:771	somatic cell score	754:771	somatic cell score	754:771	The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*).					
32229122	5	83	located	found	916:920	arg2	that					911:914	that	911:914	that	911:914	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	5	83	located	found	916:920	arg1	model					929:933	the model	925:933	the model based on milk composition and hygienic quality	925:980	The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality.					
32229122	3	84	theme	analysis	662:669	arg1	techniques					671:680	discriminant analysis techniques	649:680	discriminant analysis techniques	649:680	Based on the measured variables, we determined the differences and similarities between coagulating and noncoagulating samples using discriminant analysis techniques.					
32229122	1	85	theme	color	229:233	arg1	values					235:240	color values	229:240	color values of milk	229:248	This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation.					
32229122	10	86	theme	lesser	1615:1620	arg1	extent					1622:1627	a lesser extent	1613:1627	a lesser extent	1613:1627	The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk.					
32229122	9	87	theme	color	1314:1318	arg1	values					1320:1325	color values	1314:1325	color values	1314:1325	The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score.					
34666130	5	0	theme	electron	744:751	arg1	microscopy					753:762	Scanning electron microscopy	735:762	Scanning electron microscopy	735:762	Scanning electron microscopy revealed kaolin to be well dispersed and embedded within the starch matrix.					
34666130	6	1	theme	10 wt	889:893	arg1	kaolin					896:901	10 wt% kaolin	889:901	10 wt% kaolin	889:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	3	2	theme	10 wt	406:410	arg1	kaolin					413:418	10 wt% kaolin	406:418	10 wt% kaolin	406:418	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	1	3	theme	cassava	141:147	arg1	starch					149:154	native cassava starch	134:154	native cassava starch	134:154	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	6	4	theme	non-biodegradable	955:971	arg1	plastics					983:990	non-biodegradable synthetic plastics	955:990	non-biodegradable synthetic plastics for packaging	955:1004	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	1	5	link	crosslinked	202:212	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	5	link	crosslinked	202:212	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	6	theme	starch	149:154	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	6	theme	starch	149:154	arg1	films					125:129	Starch blend films	112:129	Starch blend films of native cassava starch	112:154	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	6	theme	starch	149:154	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	3	7	dep	solubility	519:528	arg1	%					538:538	31.60%	533:538	water solubility to 31.60%	513:538	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	3	7	dep	solubility	519:528	arg1	to					530:531	to	530:531	to	530:531	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	6	8	theme	synthetic	973:981	arg1	plastics					983:990	non-biodegradable synthetic plastics	955:990	non-biodegradable synthetic plastics for packaging	955:1004	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	3	9	theme	starch	427:432	arg1	film					440:443	the starch blend film	423:443	the starch blend film	423:443	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	1	10	theme	solution	247:254	arg1	casting					256:262	solution casting	247:262	solution casting	247:262	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	6	11	theme	film	869:872	arg1	composite					874:882	the starch blend film composite	852:882	the starch blend film composite with 10 wt% kaolin	852:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	3	12	theme	water	453:457	arg1	permeability					465:476	water vapor permeability	453:476	water vapor permeability	453:476	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	3	13	theme	vapor	459:463	arg1	permeability					465:476	water vapor permeability	453:476	water vapor permeability	453:476	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	2	14	theme	films	365:369	arg1	resistance					308:317	the water resistance	298:317	the water resistance	298:317	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	2	14	theme	films	365:369	arg1	properties					334:343	mechanical properties	323:343	mechanical properties of the starch blend films	323:369	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	3	15	theme	kaolin	413:418	arg1	addition					394:401	The addition	390:401	The addition of 10 wt% kaolin to the starch blend film	390:443	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	0	16	theme	food	96:99	arg1	packaging					101:109	food packaging	96:109	food packaging	96:109	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	6	17	theme	starch	856:861	arg1	composite					874:882	the starch blend film composite	852:882	the starch blend film composite with 10 wt% kaolin	852:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	3	18	theme	%	411:411	arg1	kaolin					413:418	10 wt% kaolin	406:418	10 wt% kaolin	406:418	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	1	19	theme	medium	160:165	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	19	theme	medium	160:165	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	3	20	theme	water	513:517	arg1	3.51 × 10-5 g m day-1 m-2 Pa-1					481:510	3.51 × 10-5 g m day-1 m-2 Pa-1	481:510	3.51 × 10-5 g m day-1 m-2 Pa-1	481:510	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	3	20	theme	water	513:517	arg1	solubility					519:528	water solubility	513:528	water solubility to 31.60%	513:538	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	3	21	theme	tensile	551:557	arg1	strength					559:566	tensile strength	551:566	tensile strength	551:566	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	6	22	theme	blend	863:867	arg1	composite					874:882	the starch blend film composite	852:882	the starch blend film composite with 10 wt% kaolin	852:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	1	23	theme	distarch	167:174	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	23	theme	distarch	167:174	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	0	24	theme	Mechanical	0:9	arg1	properties					23:32	Mechanical and barrier properties	0:32	properties	23:32	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	6	25	dep	material	935:942	arg1	replace					947:953	replace	947:953	replace	947:953	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	4	26	theme	thermal	703:709	arg1	stability					711:719	thermal stability	703:719	thermal stability	703:719	At this loading of kaolin, the structural integrity of the starch blend film was maintained during immersion in water and thermal stability was enhanced.					
34666130	1	27	theme	phosphate	176:184	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	27	theme	phosphate	176:184	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	2	28	theme	content	287:293	arg1	effects					269:275	The effects	265:275	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films	265:369	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	1	29	theme	cassava	186:192	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	29	theme	cassava	186:192	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	0	30	theme	barrier	15:21	arg1	properties					23:32	Mechanical and barrier properties	0:32	properties	23:32	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	2	31	theme	kaolin	280:285	arg1	content					287:293	kaolin content	280:293	kaolin content	280:293	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	6	32	theme	food	1032:1035	arg1	products					1037:1044	food products	1032:1044	food products	1032:1044	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	0	33	theme	starch	37:42	arg1	films					50:54	starch blend films	37:54	starch blend films	37:54	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	4	34	theme	blend	647:651	arg1	film					653:656	the starch blend film	636:656	the starch blend film	636:656	At this loading of kaolin, the structural integrity of the starch blend film was maintained during immersion in water and thermal stability was enhanced.					
34666130	4	35	theme	structural	612:621	arg1	integrity					623:631	the structural integrity	608:631	the structural integrity of the starch blend film	608:656	At this loading of kaolin, the structural integrity of the starch blend film was maintained during immersion in water and thermal stability was enhanced.					
34666130	2	36	theme	blend	359:363	arg1	films					365:369	the starch blend films	348:369	the starch blend films	348:369	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	5	37	theme	starch	825:830	arg1	matrix					832:837	the starch matrix	821:837	the starch matrix	821:837	Scanning electron microscopy revealed kaolin to be well dispersed and embedded within the starch matrix.					
34666130	1	38	theme	crosslinked	202:212	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	38	theme	crosslinked	202:212	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	0	39	theme	films	50:54	arg1	properties					23:32	Mechanical and barrier properties	0:32	properties	23:32	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	6	40	theme	interesting	907:917	arg1	properties					919:928	interesting properties	907:928	interesting properties	907:928	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	6	40	theme	interesting	907:917	arg1	material					935:942	a material	933:942	a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products	933:1044	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	2	41	theme	mechanical	323:332	arg1	properties					334:343	mechanical properties	323:343	mechanical properties of the starch blend films	323:369	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	0	42	theme	blend	44:48	arg1	films					50:54	starch blend films	37:54	starch blend films	37:54	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	1	43	theme	Starch	112:117	arg1	films					125:129	Starch blend films	112:129	Starch blend films of native cassava starch	112:154	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	4	44	theme	starch	640:645	arg1	film					653:656	the starch blend film	636:656	the starch blend film	636:656	At this loading of kaolin, the structural integrity of the starch blend film was maintained during immersion in water and thermal stability was enhanced.					
34666130	4	45	theme	film	653:656	arg1	integrity					623:631	the structural integrity	608:631	the structural integrity of the starch blend film	608:656	At this loading of kaolin, the structural integrity of the starch blend film was maintained during immersion in water and thermal stability was enhanced.					
34666130	1	46	theme	blend	119:123	arg1	films					125:129	Starch blend films	112:129	Starch blend films of native cassava starch	112:154	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	47	theme	cassava	214:220	arg1	starch					194:199	medium distarch phosphate cassava starch	160:199	medium distarch phosphate cassava starch (crosslinked cassava starch)	160:228	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	1	47	theme	cassava	214:220	arg1	starch					222:227	crosslinked cassava starch	202:227	crosslinked cassava starch	202:227	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
34666130	6	48	with	composite	874:882	arg1	kaolin					896:901	10 wt% kaolin	889:901	10 wt% kaolin	889:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	6	49	contain	had	903:905	arg2	material					935:942	a material	933:942	a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products	933:1044	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	6	49	contain	had	903:905	arg2	properties					919:928	interesting properties	907:928	interesting properties	907:928	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	6	49	contain	had	903:905	arg1	composite					874:882	the starch blend film composite	852:882	the starch blend film composite with 10 wt% kaolin	852:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	2	50	theme	water	302:306	arg1	resistance					308:317	the water resistance	298:317	the water resistance	298:317	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	0	51	from	application	81:91	arg1	packaging					101:109	food packaging	96:109	food packaging	96:109	Mechanical and barrier properties of starch blend films enhanced with kaolin for application in food packaging.					
34666130	3	52	theme	blend	434:438	arg1	film					440:443	the starch blend film	423:443	the starch blend film	423:443	The addition of 10 wt% kaolin to the starch blend film lowered water vapor permeability to 3.51 × 10-5 g m day-1 m-2 Pa-1, water solubility to 31.60% and raised tensile strength to 2.99 MPa.					
34666130	2	53	theme	starch	352:357	arg1	films					365:369	the starch blend films	348:369	the starch blend films	348:369	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	2	54	from	effects	269:275	arg1	resistance					308:317	the water resistance	298:317	the water resistance	298:317	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	2	54	from	effects	269:275	arg1	properties					334:343	mechanical properties	323:343	mechanical properties of the starch blend films	323:369	The effects of kaolin content on the water resistance and mechanical properties of the starch blend films were investigated.					
34666130	4	55	theme	kaolin	600:605	arg1	loading					589:595	this loading	584:595	this loading of kaolin	584:605	At this loading of kaolin, the structural integrity of the starch blend film was maintained during immersion in water and thermal stability was enhanced.					
34666130	5	56	theme	Scanning	735:742	arg1	microscopy					753:762	Scanning electron microscopy	735:762	Scanning electron microscopy	735:762	Scanning electron microscopy revealed kaolin to be well dispersed and embedded within the starch matrix.					
34666130	6	57	theme	%	894:894	arg1	kaolin					896:901	10 wt% kaolin	889:901	10 wt% kaolin	889:901	In summary, the starch blend film composite with 10 wt% kaolin had interesting properties as a material to replace non-biodegradable synthetic plastics for packaging, particularly sachets for food products.					
34666130	1	58	theme	native	134:139	arg1	starch					149:154	native cassava starch	134:154	native cassava starch	134:154	Starch blend films of native cassava starch and medium distarch phosphate cassava starch (crosslinked cassava starch) were prepared by solution casting.					
32458452	5	0	theme	Griess	587:592	arg1	method					594:599	Griess method	587:599	Griess method	587:599	Cell survival rate and nitrite concentration were determined using the MTT assay and Griess method, respectively.					
32458452	9	1	theme	CD	1133:1134	arg1	effect					1123:1128	the anti-inflammatory effect	1101:1128	the anti-inflammatory effect of CD	1101:1134	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	12	2	theme	herbal	1361:1366	arg1	foods					1368:1372	herbal foods	1361:1372	herbal foods	1361:1372	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	2	3	theme	anti-inflammatory	221:237	arg1	effects					239:245	anti-inflammatory effects	221:245	anti-inflammatory effects	221:245	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	7	4	theme	associated	758:767	arg1	genes					769:773	inflammatory associated genes	745:773	inflammatory associated genes	745:773	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	14	5	theme	theoretical	1751:1761	arg1	basis					1763:1767	a theoretical basis	1749:1767	a theoretical basis for the further reasonable application of Beopje processing method	1749:1834	It also provides a theoretical basis for the further reasonable application of Beopje processing method.					
32458452	11	6	theme	body	1282:1285	arg1	inflammation					1287:1298	body inflammation	1282:1298	body inflammation	1282:1298	Therefore, it is necessary to suppress the occurrence of body inflammation in time.					
32458452	13	7	theme	significant	1557:1567	arg1	research					1533:1540	This research	1528:1540	This research	1528:1540	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	7	theme	significant	1557:1567	arg1	reference					1569:1577	a significant reference	1555:1577	a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity	1555:1729	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	4	8	theme	Beopje	392:397	arg1	extracts					417:424	Beopje curly dock (CD-B) extracts	392:424	Beopje curly dock (CD-B) extracts	392:424	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	0	9	theme	active	105:110	arg1	compounds					112:120	its active compounds	101:120	its active compounds	101:120	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	6	10	theme	inflammatory	673:684	arg1	levels					695:700	the inflammatory cytokine levels	669:700	the inflammatory cytokine levels	669:700	Enzyme-linked immunosorbent assay was used to detect the inflammatory cytokine levels.					
32458452	4	11	theme	dock	405:408	arg1	extracts					417:424	Beopje curly dock (CD-B) extracts	392:424	Beopje curly dock (CD-B) extracts	392:424	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	9	12	theme	inhibitory	958:967	arg1	effect					969:974	a more significant inhibitory effect	939:974	a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD	939:1042	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	2	13	theme	Curly	182:186	arg1	food					211:214	an herbal food	201:214	an herbal food with anti-inflammatory effects	201:245	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	2	13	theme	Curly	182:186	arg1	CD					194:195	CD	194:195	CD	194:195	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	2	13	theme	Curly	182:186	arg1	dock					188:191	Curly dock	182:191	Curly dock (CD)	182:196	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	4	14	from	effects	374:380	arg1	damage					475:480	lipopolysaccharide (LPS)-induced inflammatory damage	429:480	lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells	429:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	2	15	with	food	211:214	arg1	effects					239:245	anti-inflammatory effects	221:245	anti-inflammatory effects	221:245	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	3	16	theme	processing	273:282	arg1	Beopje					248:253	Beopje	248:253	Beopje	248:253	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	3	16	theme	processing	273:282	arg1	method					284:289	an herbal food processing method	258:289	an herbal food processing method that reduces toxicity and enhances beneficial effects	258:343	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	7	17	theme	mRNA	707:710	arg1	levels					735:740	The mRNA and protein expression levels	703:740	The mRNA and protein expression levels of inflammatory associated genes	703:773	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	9	18	theme	LPS-induced	983:993	arg1	response					1008:1015	the LPS-induced inflammatory response	979:1015	the LPS-induced inflammatory response in RAW 264.7 cells	979:1034	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	3	19	theme	herbal	261:266	arg1	Beopje					248:253	Beopje	248:253	Beopje	248:253	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	3	19	theme	herbal	261:266	arg1	method					284:289	an herbal food processing method	258:289	an herbal food processing method that reduces toxicity and enhances beneficial effects	258:343	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	13	20	theme	functional	1682:1691	arg1	foods					1693:1697	functional foods	1682:1697	functional foods with anti-inflammatory activity	1682:1729	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	5	21	theme	nitrite	525:531	arg1	concentration					533:545	nitrite concentration	525:545	nitrite concentration	525:545	Cell survival rate and nitrite concentration were determined using the MTT assay and Griess method, respectively.					
32458452	14	22	theme	method	1829:1834	arg1	application					1796:1806	the further reasonable application	1773:1806	the further reasonable application of Beopje processing method	1773:1834	It also provides a theoretical basis for the further reasonable application of Beopje processing method.					
32458452	10	23	theme	chronic	1207:1213	arg1	diseases					1215:1222	chronic diseases	1207:1222	chronic diseases	1207:1222	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.					
32458452	0	24	theme	LPS-induced	69:79	arg1	cells					91:95	LPS-induced RAW 264.7 cells	69:95	LPS-induced RAW 264.7 cells	69:95	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	12	25	theme	anti-inflammatory	1502:1518	arg1	effect					1520:1525	the anti-inflammatory effect	1498:1525	the anti-inflammatory effect	1498:1525	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	4	26	theme	CD-B	411:414	arg1	extracts					417:424	Beopje curly dock (CD-B) extracts	392:424	Beopje curly dock (CD-B) extracts	392:424	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	5	27	theme	Cell	502:505	arg1	rate					516:519	Cell survival rate	502:519	Cell survival rate	502:519	Cell survival rate and nitrite concentration were determined using the MTT assay and Griess method, respectively.					
32458452	4	28	theme	extracts	417:424	arg1	effects					374:380	the effects	370:380	the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells	370:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	0	29	theme	Anti-inflammatory	0:16	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of Beopje	0:34	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	14	30	theme	processing	1818:1827	arg1	method					1829:1834	Beopje processing method	1811:1834	Beopje processing method	1811:1834	It also provides a theoretical basis for the further reasonable application of Beopje processing method.					
32458452	6	31	link	Enzyme-linked	616:628	arg1	assay					644:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay was used to detect the inflammatory cytokine levels.					
32458452	4	32	theme	RAW	485:487	arg1	cells					495:499	RAW 264.7 cells	485:499	RAW 264.7 cells	485:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	9	33	from	effect	969:974	arg1	response					1008:1015	the LPS-induced inflammatory response	979:1015	the LPS-induced inflammatory response in RAW 264.7 cells	979:1034	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	6	34	theme	Enzyme-linked	616:628	arg1	assay					644:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay was used to detect the inflammatory cytokine levels.					
32458452	0	35	dep	crispus	54:60	arg1	L.					62:63	L.	62:63	L.	62:63	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	13	36	theme	diseases	1649:1656	arg1	development					1667:1677	the development	1663:1677	the development of functional foods with anti-inflammatory activity	1663:1729	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	36	theme	diseases	1649:1656	arg1	treatment					1615:1623	treatment	1615:1623	treatment	1615:1623	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	36	theme	diseases	1649:1656	arg1	prevention					1600:1609	prevention	1600:1609	prevention	1600:1609	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	36	theme	diseases	1649:1656	arg1	research					1590:1597	future research	1583:1597	future research	1583:1597	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	4	37	from	damage	475:480	arg1	cells					495:499	RAW 264.7 cells	485:499	RAW 264.7 cells	485:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	7	38	theme	expression	724:733	arg1	levels					735:740	The mRNA and protein expression levels	703:740	The mRNA and protein expression levels of inflammatory associated genes	703:773	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	4	39	theme	-induced	453:460	arg1	damage					475:480	lipopolysaccharide (LPS)-induced inflammatory damage	429:480	lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells	429:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	7	40	theme	inflammatory	745:756	arg1	genes					769:773	inflammatory associated genes	745:773	inflammatory associated genes	745:773	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	8	41	theme	MS	891:892	arg1	analysis					894:901	UPLC-Q-TOF MS analysis	880:901	UPLC-Q-TOF MS analysis	880:901	CD and CD-B extracts compositions were assessed by UPLC-Q-TOF MS analysis.					
32458452	12	42	dep	clarified	1334:1342	arg1	confirms					1447:1454	confirms	1447:1454	confirms that applying the Beopje process enhances the anti-inflammatory effect	1447:1525	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	12	42	dep	clarified	1334:1342	arg1	alleviate					1377:1385	alleviate	1377:1385	to alleviate inflammation by regulating the immune response	1374:1432	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	10	43	dep	APPLICATIONS	1147:1158	arg1	cause					1188:1192	cause	1188:1192	can cause a variety of chronic diseases	1184:1222	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.					
32458452	9	44	theme	anti-inflammatory	1105:1121	arg1	effect					1123:1128	the anti-inflammatory effect	1101:1128	the anti-inflammatory effect of CD	1101:1134	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	12	45	theme	immune	1418:1423	arg1	response					1425:1432	the immune response	1414:1432	the immune response	1414:1432	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	10	46	theme	Long-term	1161:1169	arg1	inflammation					1171:1182	Long-term inflammation	1161:1182	Long-term inflammation	1161:1182	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.					
32458452	13	47	theme	future	1583:1588	arg1	research					1590:1597	future research	1583:1597	future research	1583:1597	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	1	48	theme	defense	141:147	arg1	Inflammation					123:134	Inflammation	123:134	Inflammation	123:134	Inflammation is a defense response of the body to stimuli.					
32458452	1	48	theme	defense	141:147	arg1	response					149:156	a defense response	139:156	a defense response of the body to stimuli	139:179	Inflammation is a defense response of the body to stimuli.					
32458452	12	49	theme	foods	1368:1372	arg1	mechanism					1348:1356	the mechanism	1344:1356	the mechanism of herbal foods	1344:1372	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	11	50	theme	inflammation	1287:1298	arg1	occurrence					1268:1277	the occurrence	1264:1277	the occurrence of body inflammation in time	1264:1306	Therefore, it is necessary to suppress the occurrence of body inflammation in time.					
32458452	5	51	theme	MTT	573:575	arg1	assay					577:581	the MTT assay	569:581	the MTT assay	569:581	Cell survival rate and nitrite concentration were determined using the MTT assay and Griess method, respectively.					
32458452	8	52	dep	CD	829:830	arg1	compositions					850:861	compositions	850:861	compositions	850:861	CD and CD-B extracts compositions were assessed by UPLC-Q-TOF MS analysis.					
32458452	7	53	theme	Western	801:807	arg1	blot					809:812	Western blot	801:812	Western blot	801:812	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	14	54	theme	reasonable	1785:1794	arg1	application					1796:1806	the further reasonable application	1773:1806	the further reasonable application of Beopje processing method	1773:1834	It also provides a theoretical basis for the further reasonable application of Beopje processing method.					
32458452	1	55	theme	body	165:168	arg1	Inflammation					123:134	Inflammation	123:134	Inflammation	123:134	Inflammation is a defense response of the body to stimuli.					
32458452	1	55	theme	body	165:168	arg1	response					149:156	a defense response	139:156	a defense response of the body to stimuli	139:179	Inflammation is a defense response of the body to stimuli.					
32458452	4	56	theme	curly	399:403	arg1	extracts					417:424	Beopje curly dock (CD-B) extracts	392:424	Beopje curly dock (CD-B) extracts	392:424	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	9	57	theme	significant	946:956	arg1	effect					969:974	a more significant inhibitory effect	939:974	a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD	939:1042	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	14	58	theme	Beopje	1811:1816	arg1	method					1829:1834	Beopje processing method	1811:1834	Beopje processing method	1811:1834	It also provides a theoretical basis for the further reasonable application of Beopje processing method.					
32458452	2	59	theme	herbal	204:209	arg1	food					211:214	an herbal food	201:214	an herbal food with anti-inflammatory effects	201:245	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	2	59	theme	herbal	204:209	arg1	dock					188:191	Curly dock	182:191	Curly dock (CD)	182:196	Curly dock (CD) is an herbal food with anti-inflammatory effects.					
32458452	9	60	theme	RAW	1020:1022	arg1	cells					1030:1034	RAW 264.7 cells	1020:1034	RAW 264.7 cells	1020:1034	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	9	61	theme	inflammatory	995:1006	arg1	response					1008:1015	the LPS-induced inflammatory response	979:1015	the LPS-induced inflammatory response in RAW 264.7 cells	979:1034	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	4	62	theme	CD	385:386	arg1	effects					374:380	the effects	370:380	the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells	370:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	3	63	theme	food	268:271	arg1	Beopje					248:253	Beopje	248:253	Beopje	248:253	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	3	63	theme	food	268:271	arg1	method					284:289	an herbal food processing method	258:289	an herbal food processing method that reduces toxicity and enhances beneficial effects	258:343	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	13	64	theme	foods	1693:1697	arg1	development					1667:1677	the development	1663:1677	the development of functional foods with anti-inflammatory activity	1663:1729	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	64	theme	foods	1693:1697	arg1	treatment					1615:1623	treatment	1615:1623	treatment	1615:1623	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	64	theme	foods	1693:1697	arg1	prevention					1600:1609	prevention	1600:1609	prevention	1600:1609	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	64	theme	foods	1693:1697	arg1	research					1590:1597	future research	1583:1597	future research	1583:1597	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	13	65	with	foods	1693:1697	arg1	activity					1722:1729	anti-inflammatory activity	1704:1729	anti-inflammatory activity	1704:1729	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	10	66	theme	diseases	1215:1222	arg1	variety					1196:1202	a variety	1194:1202	a variety of chronic diseases	1194:1222	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.					
32458452	10	66	theme	diseases	1215:1222	arg1	diseases					1215:1222	chronic diseases	1207:1222	chronic diseases	1207:1222	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.					
32458452	0	67	theme	RAW	81:83	arg1	cells					91:95	LPS-induced RAW 264.7 cells	69:95	LPS-induced RAW 264.7 cells	69:95	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	13	68	theme	anti-inflammatory	1704:1720	arg1	activity					1722:1729	anti-inflammatory activity	1704:1729	anti-inflammatory activity	1704:1729	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	9	69	from	response	1008:1015	arg1	cells					1030:1034	RAW 264.7 cells	1020:1034	RAW 264.7 cells	1020:1034	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	5	70	theme	survival	507:514	arg1	rate					516:519	Cell survival rate	502:519	Cell survival rate	502:519	Cell survival rate and nitrite concentration were determined using the MTT assay and Griess method, respectively.					
32458452	6	71	theme	immunosorbent	630:642	arg1	assay					644:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay was used to detect the inflammatory cytokine levels.					
32458452	0	72	theme	Beopje	29:34	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of Beopje	0:34	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	7	73	theme	genes	769:773	arg1	levels					735:740	The mRNA and protein expression levels	703:740	The mRNA and protein expression levels of inflammatory associated genes	703:773	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	6	74	used	used	654:657	arg2	assay					644:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay	616:648	Enzyme-linked immunosorbent assay was used to detect the inflammatory cytokine levels.					
32458452	8	75	theme	CD-B	836:839	arg1	extracts					841:848	CD-B extracts	836:848	CD-B extracts	836:848	CD and CD-B extracts compositions were assessed by UPLC-Q-TOF MS analysis.					
32458452	3	76	theme	beneficial	326:335	arg1	effects					337:343	beneficial effects	326:343	beneficial effects	326:343	Beopje is an herbal food processing method that reduces toxicity and enhances beneficial effects.					
32458452	13	77	theme	inflammation-related	1628:1647	arg1	diseases					1649:1656	inflammation-related diseases	1628:1656	inflammation-related diseases	1628:1656	This research can serve as a significant reference for future research, prevention and treatment of inflammation-related diseases, and the development of functional foods with anti-inflammatory activity.					
32458452	11	78	from	occurrence	1268:1277	arg1	time					1303:1306	time	1303:1306	time	1303:1306	Therefore, it is necessary to suppress the occurrence of body inflammation in time.					
32458452	9	79	contain	has	935:937	arg1	CD-B					930:933	CD-B	930:933	CD-B	930:933	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	9	79	contain	has	935:937	arg2	effect					969:974	a more significant inhibitory effect	939:974	a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD	939:1042	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	4	80	theme	inflammatory	462:473	arg1	damage					475:480	lipopolysaccharide (LPS)-induced inflammatory damage	429:480	lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells	429:499	This study investigated the effects of CD and Beopje curly dock (CD-B) extracts on lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells.					
32458452	9	81	theme	Beopje	1065:1070	arg1	process					1072:1078	the Beopje process	1061:1078	the Beopje process	1061:1078	Our results indicate that CD-B has a more significant inhibitory effect on the LPS-induced inflammatory response in RAW 264.7 cells than CD, suggesting that the Beopje process potentially enhances the anti-inflammatory effect of CD.					
32458452	0	82	theme	Rumex	48:52	arg1	crispus					54:60	Rumex crispus	48:60	Rumex crispus	48:60	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	14	83	theme	further	1777:1783	arg1	application					1796:1806	the further reasonable application	1773:1806	the further reasonable application of Beopje processing method	1773:1834	It also provides a theoretical basis for the further reasonable application of Beopje processing method.					
32458452	12	84	theme	Beopje	1474:1479	arg1	process					1481:1487	the Beopje process	1470:1487	the Beopje process	1470:1487	This study preliminarily clarified the mechanism of herbal foods to alleviate inflammation by regulating the immune response, and further confirms that applying the Beopje process enhances the anti-inflammatory effect.					
32458452	0	85	dep	dock	42:45	arg1	crispus					54:60	Rumex crispus	48:60	Rumex crispus	48:60	Anti-inflammatory effects of Beopje curly dock (Rumex crispus L.) in LPS-induced RAW 264.7 cells and its active compounds.					
32458452	7	86	theme	protein	716:722	arg1	expression					724:733	protein expression	716:733	protein expression	716:733	The mRNA and protein expression levels of inflammatory associated genes were detected by qPCR and Western blot, respectively.					
32458452	8	87	theme	UPLC-Q-TOF	880:889	arg1	analysis					894:901	UPLC-Q-TOF MS analysis	880:901	UPLC-Q-TOF MS analysis	880:901	CD and CD-B extracts compositions were assessed by UPLC-Q-TOF MS analysis.					
32458452	6	88	theme	cytokine	686:693	arg1	levels					695:700	the inflammatory cytokine levels	669:700	the inflammatory cytokine levels	669:700	Enzyme-linked immunosorbent assay was used to detect the inflammatory cytokine levels.					
32458452	10	89	theme	PRACTICAL	1137:1145	arg1	APPLICATIONS					1147:1158	PRACTICAL APPLICATIONS	1137:1158	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.	1137:1223	PRACTICAL APPLICATIONS: Long-term inflammation can cause a variety of chronic diseases.					
33096669	7	0	theme	FA	1105:1106	arg1	risks					1096:1100	the risks	1092:1100	the risks of FA, eczema and asthma	1092:1125	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	8	1	theme	major	1372:1376	arg1	HMOs					1378:1381	16-20 major HMOs	1366:1381	16-20 major HMOs	1366:1381	Furthermore, current analytical methods primarily used today only allow detection of 16-20 major HMOs while more than 100 types have been identified.					
33096669	4	2	theme	gut	602:604	arg1	mucosa					606:611	gut mucosa	602:611	gut mucosa	602:611	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	4	3	theme	infant	508:513	arg1	system					522:527	the infant immune system	504:527	the infant immune system	504:527	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	0	4	theme	Food	75:78	arg1	Allergy					80:86	Food Allergy	75:86	Food Allergy	75:86	Do Human Milk Oligosaccharides Protect Against Infant Atopic Disorders and Food Allergy?					
33096669	5	5	theme	microbiome	668:677	arg1	composition					679:689	microbiome composition	668:689	microbiome composition	668:689	Differences in microbiome composition between allergic and healthy infants have been observed, regardless of breastfeeding history.					
33096669	6	6	theme	randomising	966:976	arg1	individuals					978:988	randomising individuals	966:988	randomising individuals to breastfeeding	966:1005	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	9	7	from	AD	1591:1592	arg1	life					1610:1613	life	1610:1613	life	1610:1613	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	1	8	with	coexistent	118:127	arg1	allergy					139:145	food allergy	134:145	food allergy	134:145	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	3	9	from	milk	444:447	arg1	abundant					426:433	abundant	426:433	abundant	426:433	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	4	10	theme	system	522:527	arg1	development					489:499	the development	485:499	the development of the infant immune system	485:527	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	6	11	theme	limited	794:800	arg1	studies					802:808	limited studies	794:808	limited studies	794:808	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	1	12	theme	food	134:137	arg1	allergy					139:145	food allergy	134:145	food allergy	134:145	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	4	13	theme	immune	515:520	arg1	system					522:527	the infant immune system	504:527	the infant immune system	504:527	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	9	14	theme	changes	1543:1549	arg1	impact					1533:1538	the impact	1529:1538	the impact of changes over the lactation period	1529:1575	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	9	14	theme	changes	1543:1549	arg1	composition					1513:1523	HMO composition	1509:1523	HMO composition	1509:1523	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	6	15	theme	HMO	947:949	arg1	intake					951:956	HMO intake	947:956	HMO intake	947:956	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	5	16	theme	allergic	699:706	arg1	infants					720:726	allergic and healthy infants	699:726	allergic and healthy infants	699:726	Differences in microbiome composition between allergic and healthy infants have been observed, regardless of breastfeeding history.					
33096669	1	17	theme	health	202:207	arg1	consequences					209:220	lifelong health consequences	193:220	lifelong health consequences	193:220	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	5	18	theme	breastfeeding	762:774	arg1	history					776:782	breastfeeding history	762:782	breastfeeding history	762:782	Differences in microbiome composition between allergic and healthy infants have been observed, regardless of breastfeeding history.					
33096669	0	19	theme	Human	3:7	arg1	Oligosaccharides					14:29	Human Milk Oligosaccharides	3:29	Human Milk Oligosaccharides	3:29	Do Human Milk Oligosaccharides Protect Against Infant Atopic Disorders and Food Allergy?					
33096669	9	20	theme	large-scale	1436:1446	arg1	studies					1461:1467	More large-scale longitudinal studies	1431:1467	More large-scale longitudinal studies	1431:1467	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	7	21	theme	breastfeeding	1066:1078	arg1	effects					1055:1061	beneficial effects	1044:1061	beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma	1044:1125	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	3	22	theme	milk	362:365	arg1	HMOs					385:388	HMOs	385:388	HMOs	385:388	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	3	22	theme	milk	362:365	arg1	oligosaccharides					367:382	Human milk oligosaccharides	356:382	Human milk oligosaccharides (HMOs)	356:389	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	3	22	theme	milk	362:365	arg1	carbohydrates					403:415	complex carbohydrates	395:415	complex carbohydrates that are abundant in human milk	395:447	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	5	23	theme	healthy	712:718	arg1	infants					720:726	allergic and healthy infants	699:726	allergic and healthy infants	699:726	Differences in microbiome composition between allergic and healthy infants have been observed, regardless of breastfeeding history.					
33096669	9	24	theme	lactation	1560:1568	arg1	period					1570:1575	the lactation period	1556:1575	the lactation period	1556:1575	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	3	25	from	abundant	426:433	arg1	milk					444:447	human milk	438:447	human milk	438:447	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	1	26	theme	Atopic	89:94	arg1	AD					107:108	AD	107:108	AD	107:108	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	1	26	theme	Atopic	89:94	arg1	disorders					96:104	Atopic disorders	89:104	Atopic disorders (AD)	89:109	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	1	26	theme	Atopic	89:94	arg1	coexistent					118:127	coexistent	118:127	often coexistent with food allergy (FA)	112:150	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	6	27	theme	current	883:889	arg1	data					891:894	current data	883:894	current data	883:894	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	7	28	theme	diagnostic	1207:1216	arg1	breastfeeding					1192:1204	breastfeeding	1192:1204	breastfeeding	1192:1204	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	7	28	theme	diagnostic	1207:1216	arg1	criteria					1218:1225	diagnostic criteria	1207:1225	diagnostic criteria for AD	1207:1232	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	7	29	from	studies	1165:1171	arg1	age					1242:1244	the age	1238:1244	the age at which the outcome was assessed	1238:1278	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	7	29	from	studies	1165:1171	arg1	duration					1180:1187	the duration	1176:1187	the duration of breastfeeding, diagnostic criteria for AD	1176:1232	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	4	30	theme	immune	638:643	arg1	system					645:650	the immune system	634:650	the immune system	634:650	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	3	31	theme	complex	395:401	arg1	oligosaccharides					367:382	Human milk oligosaccharides	356:382	Human milk oligosaccharides (HMOs)	356:389	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	3	31	theme	complex	395:401	arg1	carbohydrates					403:415	complex carbohydrates	395:415	complex carbohydrates that are abundant in human milk	395:447	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	0	32	theme	Milk	9:12	arg1	Oligosaccharides					14:29	Human Milk Oligosaccharides	3:29	Human Milk Oligosaccharides	3:29	Do Human Milk Oligosaccharides Protect Against Infant Atopic Disorders and Food Allergy?					
33096669	9	33	theme	longitudinal	1448:1459	arg1	studies					1461:1467	More large-scale longitudinal studies	1431:1467	More large-scale longitudinal studies	1431:1467	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	7	34	theme	breastfeeding	1192:1204	arg1	age					1242:1244	the age	1238:1244	the age at which the outcome was assessed	1238:1278	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	7	34	theme	breastfeeding	1192:1204	arg1	duration					1180:1187	the duration	1176:1187	the duration of breastfeeding, diagnostic criteria for AD	1176:1232	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	3	35	theme	human	438:442	arg1	milk					444:447	human milk	438:447	human milk	438:447	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	3	36	theme	Human	356:360	arg1	HMOs					385:388	HMOs	385:388	HMOs	385:388	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	3	36	theme	Human	356:360	arg1	oligosaccharides					367:382	Human milk oligosaccharides	356:382	Human milk oligosaccharides (HMOs)	356:389	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	3	36	theme	Human	356:360	arg1	carbohydrates					403:415	complex carbohydrates	395:415	complex carbohydrates that are abundant in human milk	395:447	Human milk oligosaccharides (HMOs) are complex carbohydrates that are abundant in human milk.					
33096669	6	37	theme	HMOs	850:853	arg1	effects					839:845	the preventive effects	824:845	the preventive effects of HMOs on AD and FA	824:866	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	7	38	theme	beneficial	1044:1053	arg1	effects					1055:1061	beneficial effects	1044:1061	beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma	1044:1125	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	1	39	contain	have	188:191	arg2	consequences					209:220	lifelong health consequences	193:220	lifelong health consequences	193:220	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	1	39	contain	have	188:191	arg1	disorders					96:104	Atopic disorders	89:104	Atopic disorders (AD)	89:109	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	1	39	contain	have	188:191	arg1	AD					107:108	AD	107:108	AD	107:108	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	1	39	contain	have	188:191	arg1	coexistent					118:127	coexistent	118:127	often coexistent with food allergy (FA)	112:150	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	1	40	theme	early	173:177	arg1	life					179:182	early life	173:182	early life	173:182	Atopic disorders (AD), often coexistent with food allergy (FA), start developing in early life and have lifelong health consequences.					
33096669	0	41	theme	Atopic	54:59	arg1	Disorders					61:69	Infant Atopic Disorders	47:69	Infant Atopic Disorders	47:69	Do Human Milk Oligosaccharides Protect Against Infant Atopic Disorders and Food Allergy?					
33096669	6	42	from	effects	839:845	arg1	AD					858:859	AD	858:859	AD	858:859	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	6	42	from	effects	839:845	arg1	FA					865:866	FA	865:866	FA	865:866	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	0	43	theme	Infant	47:52	arg1	Disorders					61:69	Infant Atopic Disorders	47:69	Infant Atopic Disorders	47:69	Do Human Milk Oligosaccharides Protect Against Infant Atopic Disorders and Food Allergy?					
33096669	8	44	theme	HMOs	1378:1381	arg1	detection					1353:1361	detection	1353:1361	detection of 16-20 major HMOs	1353:1381	Furthermore, current analytical methods primarily used today only allow detection of 16-20 major HMOs while more than 100 types have been identified.					
33096669	5	45	from	Differences	653:663	arg1	composition					679:689	microbiome composition	668:689	microbiome composition	668:689	Differences in microbiome composition between allergic and healthy infants have been observed, regardless of breastfeeding history.					
33096669	4	46	dep	promoting	536:544	arg1	i					533:533	i	533:533	i	533:533	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	9	47	theme	impact	1533:1538	arg1	role					1501:1504	the role	1497:1504	the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life	1497:1613	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	6	48	theme	preventive	828:837	arg1	effects					839:845	the preventive effects	824:845	the preventive effects of HMOs on AD and FA	824:866	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	8	49	theme	current	1294:1300	arg1	methods					1313:1319	current analytical methods	1294:1319	current analytical methods primarily used	1294:1334	Furthermore, current analytical methods primarily used today only allow detection of 16-20 major HMOs while more than 100 types have been identified.					
33096669	4	50	dep	inhibiting	571:580	arg1	ii					567:568	ii	567:568	ii	567:568	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	9	51	theme	composition	1513:1523	arg1	role					1501:1504	the role	1497:1504	the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life	1497:1613	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	4	52	dep	modulating	623:632	arg1	iii					618:620	iii	618:620	iii	618:620	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	6	53	theme	observation	906:916	arg1	studies					918:924	observation studies	906:924	observation studies	906:924	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	7	54	theme	eczema	1109:1114	arg1	risks					1096:1100	the risks	1092:1100	the risks of FA, eczema and asthma	1092:1125	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	9	55	theme	HMO	1509:1511	arg1	composition					1513:1523	HMO composition	1509:1523	HMO composition	1509:1523	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
33096669	4	56	theme	healthy	546:552	arg1	microbiome					554:563	healthy microbiome	546:563	healthy microbiome	546:563	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	6	57	dep	infants	871:877	arg1	relies					896:901	relies	896:901	relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical	896:1019	To date, limited studies have examined the preventive effects of HMOs on AD and FA in infants and current data relies on observation studies as trials of varying HMO intake through randomising individuals to breastfeeding are unethical.					
33096669	8	58	theme	analytical	1302:1311	arg1	methods					1313:1319	current analytical methods	1294:1319	current analytical methods primarily used	1294:1334	Furthermore, current analytical methods primarily used today only allow detection of 16-20 major HMOs while more than 100 types have been identified.					
33096669	4	59	theme	pathogen	582:589	arg1	binding					591:597	pathogen binding	582:597	pathogen binding to gut mucosa	582:611	These are thought to contribute to the development of the infant immune system by (i) promoting healthy microbiome, (ii) inhibiting pathogen binding to gut mucosa and (iii) modulating the immune system.					
33096669	7	60	theme	asthma	1120:1125	arg1	risks					1096:1100	the risks	1092:1100	the risks of FA, eczema and asthma	1092:1125	There is evidence for beneficial effects of breastfeeding on lowering the risks of FA, eczema and asthma but there are inconsistencies amongst studies in the duration of breastfeeding, diagnostic criteria for AD and the age at which the outcome was assessed.					
33096669	9	61	from	FA	1598:1599	arg1	life					1610:1613	life	1610:1613	life	1610:1613	More large-scale longitudinal studies are required to investigate the role of HMO composition and the impact of changes over the lactation period in preventing AD and FA later in life.					
34028188	8	0	theme	good	1185:1188	arg1	area					1201:1204	good dispersion area	1185:1204	good dispersion area	1185:1204	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	11	1	dep	cements	1613:1619	arg1	modified					1621:1628	modified	1621:1628	cements modified by different starches also performed differently in surgical models for osteoporotic vertebral fracture	1613:1732	Bone cements modified by different starches also performed differently in surgical models for osteoporotic vertebral fracture.					
34028188	2	2	theme	starch	355:360	arg1	type					362:365	starch type	355:365	starch type	355:365	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	1	3	theme	pregelatinized	251:264	arg1	starches					293:300	native and pregelatinized normal corn and waxy maize starches	240:300	native and pregelatinized normal corn and waxy maize starches	240:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	12	4	theme	pregelatinized	1832:1845	arg1	starch					1859:1864	the pregelatinized normal corn starch	1828:1864	the pregelatinized normal corn starch	1828:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	3	5	theme	maize	512:516	arg1	starch					518:523	pregelatinized waxy maize starch	492:523	pregelatinized waxy maize starch (CPB-PW)	492:532	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	3	5	theme	maize	512:516	arg1	CPB-PW					526:531	CPB-PW	526:531	CPB-PW	526:531	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	1	6	theme	corn	273:276	arg1	starches					293:300	native and pregelatinized normal corn and waxy maize starches	240:300	native and pregelatinized normal corn and waxy maize starches	240:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	12	7	theme	corn	1854:1857	arg1	starch					1859:1864	the pregelatinized normal corn starch	1828:1864	the pregelatinized normal corn starch	1828:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	8	8	theme	Sawbones	1265:1272	arg1	model					1281:1285	the Sawbones sponge model	1261:1285	the Sawbones sponge model	1261:1285	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	9	9	theme	body	1474:1477	arg1	strength					1442:1449	the compressive strength	1426:1449	the compressive strength of the sheep vertebral body	1426:1477	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	8	10	theme	improved	1215:1222	arg1	force					1233:1237	improved pull-out force	1215:1237	improved pull-out force	1215:1237	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	0	11	theme	vertebral	150:158	arg1	fracture					160:167	the vertebral fracture	146:167	the vertebral fracture surgical models	146:183	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	3	12	theme	surgical	574:581	arg1	models					583:588	two vertebral fracture surgical models	551:588	two vertebral fracture surgical models	551:588	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	1	13	theme	Calcium	195:201	arg1	cement					213:218	Calcium phosphate cement	195:218	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches	195:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	1	13	theme	Calcium	195:201	arg1	CPC					221:223	CPC	221:223	CPC	221:223	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	3	14	theme	vertebral	555:563	arg1	fracture					565:572	vertebral fracture	555:572	two vertebral fracture surgical models	551:588	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	8	15	theme	maximum	1243:1249	arg1	torque					1251:1256	maximum torque	1243:1256	maximum torque	1243:1256	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	6	16	theme	CPC	982:984	arg1	hydro-reaction					964:977	the hydro-reaction	960:977	the hydro-reaction of CPC	960:984	Addition of starch did not inhibit the hydro-reaction of CPC.					
34028188	7	17	contain	had	1002:1004	arg1	CPC					998:1000	Unmodified CPC	987:1000	Unmodified CPC	987:1000	Unmodified CPC had very poor dispersibility and could not apply in the tests of the surgical models.					
34028188	7	17	contain	had	1002:1004	arg2	dispersibility					1016:1029	very poor dispersibility	1006:1029	very poor dispersibility	1006:1029	Unmodified CPC had very poor dispersibility and could not apply in the tests of the surgical models.					
34028188	7	18	theme	surgical	1071:1078	arg1	models					1080:1085	the surgical models	1067:1085	the surgical models	1067:1085	Unmodified CPC had very poor dispersibility and could not apply in the tests of the surgical models.					
34028188	0	19	theme	surgical	169:176	arg1	models					178:183	the vertebral fracture surgical models	146:183	the vertebral fracture surgical models	146:183	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	12	20	theme	CPC	1798:1800	arg1	modification					1802:1813	CPC modification	1798:1813	CPC modification comparing to the pregelatinized normal corn starch	1798:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	4	21	theme	CPC	704:706	arg1	times					677:681	the setting times	665:681	the setting times	665:681	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	4	21	theme	CPC	704:706	arg1	injectability					687:699	injectability	687:699	injectability of CPC	687:706	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	0	22	theme	type	59:62	arg1	Impact					42:47	Impact	42:47	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.	0:193	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	7	23	theme	poor	1011:1014	arg1	dispersibility					1016:1029	very poor dispersibility	1006:1029	very poor dispersibility	1006:1029	Unmodified CPC had very poor dispersibility and could not apply in the tests of the surgical models.					
34028188	0	24	theme	starch	68:73	arg1	pregelatinization					75:91	starch pregelatinization	68:91	starch pregelatinization	68:91	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	9	25	theme	invasive	1316:1323	arg1	model					1337:1341	the minimally invasive kyphoplasty model	1302:1341	the minimally invasive kyphoplasty model	1302:1341	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	2	26	from	Effects	315:321	arg1	properties					390:399	the physicochemical properties	370:399	the physicochemical properties of CPC	370:406	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	7	27	theme	Unmodified	987:996	arg1	CPC					998:1000	Unmodified CPC	987:1000	Unmodified CPC	987:1000	Unmodified CPC had very poor dispersibility and could not apply in the tests of the surgical models.					
34028188	12	28	theme	Pregelatinized	1735:1748	arg1	candidate					1784:1792	a better candidate	1775:1792	a better candidate for CPC modification comparing to the pregelatinized normal corn starch	1775:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	12	28	theme	Pregelatinized	1735:1748	arg1	starch					1761:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	4	29	theme	CPC	807:809	arg1	strength					795:802	the anti-collapsibility and compressive strength	755:802	the anti-collapsibility and compressive strength of CPC	755:809	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	2	30	theme	CPC	404:406	arg1	properties					390:399	the physicochemical properties	370:399	the physicochemical properties of CPC	370:406	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	0	31	theme	physicochemical	100:114	arg1	properties					116:125	its physicochemical properties	96:125	its physicochemical properties	96:125	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	12	32	theme	maize	1755:1759	arg1	candidate					1784:1792	a better candidate	1775:1792	a better candidate for CPC modification comparing to the pregelatinized normal corn starch	1775:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	12	32	theme	maize	1755:1759	arg1	starch					1761:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	0	33	theme	Calcium	0:6	arg1	cement					34:39	Calcium phosphate-based composite cement	0:39	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.	0:193	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	8	34	theme	Pregelatinized	1088:1101	arg1	starch					1103:1108	Pregelatinized starch	1088:1108	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	0	35	theme	composite	24:32	arg1	cement					34:39	Calcium phosphate-based composite cement	0:39	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.	0:193	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	1	36	theme	maize	287:291	arg1	starches					293:300	native and pregelatinized normal corn and waxy maize starches	240:300	native and pregelatinized normal corn and waxy maize starches	240:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	10	37	theme	starch	1524:1529	arg1	source					1541:1546	starch botanical source	1524:1546	starch botanical source	1524:1546	In conclusion, starch pregelatinization and starch botanical source affect the physicochemical properties of CPC significantly.					
34028188	3	38	theme	corn	467:470	arg1	starch					472:477	pregelatinized normal corn starch	445:477	pregelatinized normal corn starch (CPB-PNC)	445:487	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	3	38	theme	corn	467:470	arg1	CPB-PNC					480:486	CPB-PNC	480:486	CPB-PNC	480:486	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	8	39	theme	maize	1126:1130	arg1	starch					1132:1137	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	3	40	theme	pregelatinized	492:505	arg1	starch					518:523	pregelatinized waxy maize starch	492:523	pregelatinized waxy maize starch (CPB-PW)	492:532	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	3	40	theme	pregelatinized	492:505	arg1	CPB-PW					526:531	CPB-PW	526:531	CPB-PW	526:531	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	9	41	theme	sheep	1398:1402	arg1	vertebrae					1404:1412	the osteoporotic sheep vertebrae	1381:1412	the osteoporotic sheep vertebrae	1381:1412	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	2	42	theme	starch	326:331	arg1	pregelatinization					333:349	starch pregelatinization	326:349	starch pregelatinization	326:349	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	5	43	theme	best	892:895	arg1	properties					913:922	the best physicochemical properties	888:922	the best physicochemical properties	888:922	CPB-PW, whose micro-structure was compact and uniform, showed the best physicochemical properties.					
34028188	8	44	theme	CPC	1170:1172	arg1	dispersibility					1152:1165	the dispersibility	1148:1165	the dispersibility of CPC	1148:1172	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	10	45	theme	CPC	1589:1591	arg1	properties					1575:1584	the physicochemical properties	1555:1584	the physicochemical properties of CPC	1555:1591	In conclusion, starch pregelatinization and starch botanical source affect the physicochemical properties of CPC significantly.					
34028188	2	46	theme	type	362:365	arg1	Effects					315:321	Effects	315:321	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC	315:406	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	12	47	theme	normal	1847:1852	arg1	starch					1859:1864	the pregelatinized normal corn starch	1828:1864	the pregelatinized normal corn starch	1828:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	11	48	theme	osteoporotic	1702:1713	arg1	fracture					1725:1732	osteoporotic vertebral fracture	1702:1732	osteoporotic vertebral fracture	1702:1732	Bone cements modified by different starches also performed differently in surgical models for osteoporotic vertebral fracture.					
34028188	1	49	theme	normal	266:271	arg1	corn					273:276	normal corn	266:276	normal corn	266:276	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	8	50	theme	dispersion	1190:1199	arg1	area					1201:1204	good dispersion area	1185:1204	good dispersion area	1185:1204	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	6	51	theme	starch	937:942	arg1	Addition					925:932	Addition	925:932	Addition of starch	925:942	Addition of starch did not inhibit the hydro-reaction of CPC.					
34028188	9	52	theme	vertebral	1464:1472	arg1	body					1474:1477	the sheep vertebral body	1454:1477	the sheep vertebral body	1454:1477	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	4	53	theme	setting	669:675	arg1	times					677:681	the setting times	665:681	the setting times	665:681	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	3	54	theme	fracture	565:572	arg1	models					583:588	two vertebral fracture surgical models	551:588	two vertebral fracture surgical models	551:588	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	5	55	theme	physicochemical	897:911	arg1	properties					913:922	the best physicochemical properties	888:922	the best physicochemical properties	888:922	CPB-PW, whose micro-structure was compact and uniform, showed the best physicochemical properties.					
34028188	8	56	theme	sponge	1274:1279	arg1	model					1281:1285	the Sawbones sponge model	1261:1285	the Sawbones sponge model	1261:1285	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	1	57	theme	phosphate	203:211	arg1	cement					213:218	Calcium phosphate cement	195:218	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches	195:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	1	57	theme	phosphate	203:211	arg1	CPC					221:223	CPC	221:223	CPC	221:223	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	8	58	theme	pull-out	1224:1231	arg1	force					1233:1237	improved pull-out force	1215:1237	improved pull-out force	1215:1237	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	2	59	theme	pregelatinization	333:349	arg1	Effects					315:321	Effects	315:321	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC	315:406	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	7	60	theme	models	1080:1085	arg1	tests					1058:1062	the tests	1054:1062	the tests of the surgical models	1054:1085	Unmodified CPC had very poor dispersibility and could not apply in the tests of the surgical models.					
34028188	0	61	theme	fracture	160:167	arg1	models					178:183	the vertebral fracture surgical models	146:183	the vertebral fracture surgical models	146:183	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	0	62	from	properties	116:125	arg1	models					178:183	the vertebral fracture surgical models	146:183	the vertebral fracture surgical models	146:183	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	12	63	theme	better	1777:1782	arg1	candidate					1784:1792	a better candidate	1775:1792	a better candidate for CPC modification comparing to the pregelatinized normal corn starch	1775:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	12	63	theme	better	1777:1782	arg1	starch					1761:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	4	64	theme	pregelatinized	722:735	arg1	starches					737:744	only the pregelatinized starches	713:744	only the pregelatinized starches	713:744	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	0	65	theme	pregelatinization	75:91	arg1	Impact					42:47	Impact	42:47	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.	0:193	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	9	66	theme	kyphoplasty	1325:1335	arg1	model					1337:1341	the minimally invasive kyphoplasty model	1302:1341	the minimally invasive kyphoplasty model	1302:1341	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	4	67	theme	compressive	783:793	arg1	strength					795:802	the anti-collapsibility and compressive strength	755:802	the anti-collapsibility and compressive strength of CPC	755:809	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	9	68	theme	sheep	1458:1462	arg1	body					1474:1477	the sheep vertebral body	1454:1477	the sheep vertebral body	1454:1477	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	0	69	from	Impact	42:47	arg1	performance					131:141	performance	131:141	performance in the vertebral fracture surgical models in vitro	131:192	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	0	69	from	Impact	42:47	arg1	properties					116:125	its physicochemical properties	96:125	its physicochemical properties	96:125	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	10	70	theme	starch	1495:1500	arg1	pregelatinization					1502:1518	starch pregelatinization	1495:1518	starch pregelatinization	1495:1518	In conclusion, starch pregelatinization and starch botanical source affect the physicochemical properties of CPC significantly.					
34028188	3	71	theme	normal	460:465	arg1	starch					472:477	pregelatinized normal corn starch	445:477	pregelatinized normal corn starch (CPB-PNC)	445:487	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	3	71	theme	normal	460:465	arg1	CPB-PNC					480:486	CPB-PNC	480:486	CPB-PNC	480:486	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	0	72	theme	phosphate-based	8:22	arg1	cement					34:39	Calcium phosphate-based composite cement	0:39	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.	0:193	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	8	73	theme	starch	1103:1108	arg1	starch					1132:1137	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	12	74	theme	waxy	1750:1753	arg1	candidate					1784:1792	a better candidate	1775:1792	a better candidate for CPC modification comparing to the pregelatinized normal corn starch	1775:1864	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	12	74	theme	waxy	1750:1753	arg1	starch					1761:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch	1735:1766	Pregelatinized waxy maize starch may be a better candidate for CPC modification comparing to the pregelatinized normal corn starch.					
34028188	2	75	theme	physicochemical	374:388	arg1	properties					390:399	the physicochemical properties	370:399	the physicochemical properties of CPC	370:406	Effects of starch pregelatinization and starch type on the physicochemical properties of CPC were investigated.					
34028188	1	76	theme	waxy	282:285	arg1	starches					293:300	native and pregelatinized normal corn and waxy maize starches	240:300	native and pregelatinized normal corn and waxy maize starches	240:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	8	77	theme	waxy	1121:1124	arg1	starch					1132:1137	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch	1088:1137	Pregelatinized starch especially waxy maize starch improved the dispersibility of CPC and showed good dispersion area, volume, improved pull-out force and maximum torque in the Sawbones sponge model.					
34028188	10	78	theme	botanical	1531:1539	arg1	source					1541:1546	starch botanical source	1524:1546	starch botanical source	1524:1546	In conclusion, starch pregelatinization and starch botanical source affect the physicochemical properties of CPC significantly.					
34028188	4	79	theme	anti-collapsibility	759:777	arg1	strength					795:802	the anti-collapsibility and compressive strength	755:802	the anti-collapsibility and compressive strength of CPC	755:809	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	3	80	theme	pregelatinized	445:458	arg1	starch					472:477	pregelatinized normal corn starch	445:477	pregelatinized normal corn starch (CPB-PNC)	445:487	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	3	80	theme	pregelatinized	445:458	arg1	CPB-PNC					480:486	CPB-PNC	480:486	CPB-PNC	480:486	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	0	81	from	performance	131:141	arg1	models					178:183	the vertebral fracture surgical models	146:183	the vertebral fracture surgical models	146:183	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	0	82	theme	starch	52:57	arg1	type					59:62	starch type	52:62	starch type	52:62	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	9	83	theme	osteoporotic	1385:1396	arg1	vertebrae					1404:1412	the osteoporotic sheep vertebrae	1381:1412	the osteoporotic sheep vertebrae	1381:1412	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
34028188	10	84	theme	physicochemical	1559:1573	arg1	properties					1575:1584	the physicochemical properties	1555:1584	the physicochemical properties of CPC	1555:1591	In conclusion, starch pregelatinization and starch botanical source affect the physicochemical properties of CPC significantly.					
34028188	11	85	theme	different	1633:1641	arg1	starches					1643:1650	different starches	1633:1650	different starches also performed differently in surgical models for osteoporotic vertebral fracture	1633:1732	Bone cements modified by different starches also performed differently in surgical models for osteoporotic vertebral fracture.					
34028188	3	86	theme	waxy	507:510	arg1	starch					518:523	pregelatinized waxy maize starch	492:523	pregelatinized waxy maize starch (CPB-PW)	492:532	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	3	86	theme	waxy	507:510	arg1	CPB-PW					526:531	CPB-PW	526:531	CPB-PW	526:531	CPC modified with pregelatinized normal corn starch (CPB-PNC) or pregelatinized waxy maize starch (CPB-PW) was evaluated by two vertebral fracture surgical models in vitro.					
34028188	0	87	dep	cement	34:39	arg1	Impact					42:47	Impact	42:47	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.	0:193	Calcium phosphate-based composite cement: Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro.					
34028188	4	88	theme	granular	605:612	arg1	starches					633:640	Both granular and pregelatinized starches	600:640	Both granular and pregelatinized starches	600:640	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	11	89	theme	surgical	1682:1689	arg1	models					1691:1696	surgical models	1682:1696	surgical models for osteoporotic vertebral fracture	1682:1732	Bone cements modified by different starches also performed differently in surgical models for osteoporotic vertebral fracture.					
34028188	4	90	theme	pregelatinized	618:631	arg1	starches					633:640	Both granular and pregelatinized starches	600:640	Both granular and pregelatinized starches	600:640	Both granular and pregelatinized starches significantly improved the setting times and injectability of CPC, but only the pregelatinized starches improved the anti-collapsibility and compressive strength of CPC significantly.					
34028188	11	91	theme	vertebral	1715:1723	arg1	fracture					1725:1732	osteoporotic vertebral fracture	1702:1732	osteoporotic vertebral fracture	1702:1732	Bone cements modified by different starches also performed differently in surgical models for osteoporotic vertebral fracture.					
34028188	1	92	theme	native	240:245	arg1	starches					293:300	native and pregelatinized normal corn and waxy maize starches	240:300	native and pregelatinized normal corn and waxy maize starches	240:300	Calcium phosphate cement (CPC) modified with native and pregelatinized normal corn and waxy maize starches was studied.					
34028188	9	93	theme	compressive	1430:1440	arg1	strength					1442:1449	the compressive strength	1426:1449	the compressive strength of the sheep vertebral body	1426:1477	Similarly, in the minimally invasive kyphoplasty model, CPB-PNC and CPB-PW could disperse in the osteoporotic sheep vertebrae and improve the compressive strength of the sheep vertebral body.					
32718603	0	0	theme	tissue	100:105	arg1	engineering					107:117	tissue engineering	100:117	tissue engineering application	100:129	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	1	1	theme	in	298:299	arg1	foaming					310:316	in situ gas foaming	298:316	in situ gas foaming	298:316	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	2	2	theme	TGA	525:527	arg1	analysis					529:536	TGA analysis	525:536	TGA analysis	525:536	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	3	3	theme	physical	634:641	arg1	properties					658:667	the physical and mechanical properties	630:667	the physical and mechanical properties of composite scaffolds	630:690	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	1	4	theme	silk	231:234	arg1	SF					245:246	SF	245:246	SF	245:246	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	1	4	theme	silk	231:234	arg1	fibroin					236:242	silk fibroin	231:242	silk fibroin (SF)	231:247	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	1	5	theme	gas	306:308	arg1	foaming					310:316	in situ gas foaming	298:316	in situ gas foaming	298:316	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	5	6	theme	new	970:972	arg1	structure					877:885	Such a nanocomposite structure	856:885	Such a nanocomposite structure with its promising properties and cell-material interaction	856:945	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	5	6	theme	new	970:972	arg1	scaffold					974:981	a new scaffold	968:981	a new scaffold for different tissue engineering applications	968:1027	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	5	7	theme	promising	896:904	arg1	properties					906:915	its promising properties	892:915	its promising properties	892:915	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	0	8	theme	engineering	107:117	arg1	application					119:129	tissue engineering application	100:129	tissue engineering application	100:129	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	1	9	theme	foaming	310:316	arg1	methods					358:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	2	10	theme	composite	459:467	arg1	properties					402:411	The physicochemical and mechanical properties	367:411	properties	402:411	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	2	10	theme	composite	459:467	arg1	stability					433:441	thermal stability	425:441	thermal stability of the proposed composite	425:467	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	1	11	theme	in	319:320	arg1	crosslinking					327:338	in situ crosslinking	319:338	in situ crosslinking	319:338	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	4	12	theme	cell	764:767	arg1	attachment					778:787	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	5	13	theme	cell-material	921:933	arg1	interaction					935:945	cell-material interaction	921:945	cell-material interaction	921:945	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	1	14	theme	oxidized	189:196	arg1	alginate					198:205	oxidized alginate	189:205	oxidized alginate (OAL)	189:211	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	1	14	theme	oxidized	189:196	arg1	OAL					208:210	OAL	208:210	OAL	208:210	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	0	15	theme	porous	2:7	arg1	scaffold					40:47	A porous hydrogel-electrospun composite scaffold	0:47	A porous hydrogel-electrospun composite scaffold	0:47	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	5	16	theme	nanocomposite	863:875	arg1	structure					877:885	Such a nanocomposite structure	856:885	Such a nanocomposite structure with its promising properties and cell-material interaction	856:945	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	5	16	theme	nanocomposite	863:875	arg1	scaffold					974:981	a new scaffold	968:981	a new scaffold for different tissue engineering applications	968:1027	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	4	17	link	adipose-derived	731:745	arg1	cell					764:767	a favorable adipose-derived mesenchymal stem cell	719:767	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	4	17	link	adipose-derived	731:745	arg1	AMSC					772:775	AMSC	772:775	AMSC	772:775	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	0	18	theme	composite	30:38	arg1	scaffold					40:47	A porous hydrogel-electrospun composite scaffold	0:47	A porous hydrogel-electrospun composite scaffold	0:47	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	3	19	theme	composite	672:680	arg1	scaffolds					682:690	composite scaffolds	672:690	composite scaffolds	672:690	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	2	20	theme	physicochemical	371:385	arg1	properties					402:411	The physicochemical and mechanical properties	367:411	properties	402:411	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	0	21	theme	hydrogel-electrospun	9:28	arg1	scaffold					40:47	A porous hydrogel-electrospun composite scaffold	0:47	A porous hydrogel-electrospun composite scaffold	0:47	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	3	22	theme	mechanical	647:656	arg1	properties					658:667	the physical and mechanical properties	630:667	the physical and mechanical properties of composite scaffolds	630:690	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	1	23	theme	crosslinking	327:338	arg1	methods					358:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	2	24	theme	thermal	425:431	arg1	stability					433:441	thermal stability	425:441	thermal stability of the proposed composite	425:467	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	1	25	dep	in	298:299	arg1	situ					301:304	situ	301:304	situ	301:304	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	4	26	theme	adipose-derived	731:745	arg1	cell					764:767	a favorable adipose-derived mesenchymal stem cell	719:767	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	4	26	theme	adipose-derived	731:745	arg1	AMSC					772:775	AMSC	772:775	AMSC	772:775	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	1	27	dep	in	319:320	arg1	situ					322:325	situ	322:325	situ	322:325	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	1	28	theme	freeze	344:349	arg1	drying					351:356	freeze drying	344:356	freeze drying	344:356	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	2	29	theme	mechanical	391:400	arg1	properties					402:411	The physicochemical and mechanical properties	367:411	properties	402:411	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	0	30	theme	oxidized	57:64	arg1	fibroin					88:94	oxidized alginate/gelatin/silk fibroin	57:94	oxidized alginate/gelatin/silk fibroin for tissue engineering application	57:129	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	1	31	theme	drying	351:356	arg1	methods					358:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	4	32	theme	stem	759:762	arg1	cell					764:767	a favorable adipose-derived mesenchymal stem cell	719:767	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	4	32	theme	stem	759:762	arg1	AMSC					772:775	AMSC	772:775	AMSC	772:775	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	3	33	theme	vital	606:610	arg1	role					612:615	a vital role	604:615	a vital role	604:615	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	3	34	theme	scaffolds	682:690	arg1	properties					658:667	the physical and mechanical properties	630:667	the physical and mechanical properties of composite scaffolds	630:690	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	3	35	theme	crosslinking	586:597	arg1	structure					562:570	structure	562:570	structure	562:570	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	3	35	theme	crosslinking	586:597	arg1	degree					576:581	degree	576:581	degree	576:581	The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds.					
32718603	4	36	theme	mesenchymal	747:757	arg1	cell					764:767	a favorable adipose-derived mesenchymal stem cell	719:767	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	4	36	theme	mesenchymal	747:757	arg1	AMSC					772:775	AMSC	772:775	AMSC	772:775	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	1	37	theme	electrospinning	281:295	arg1	methods					358:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods	281:364	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	4	38	theme	hydrogel-electrospun	824:843	arg1	composite					845:853	this novel hydrogel-electrospun composite	813:853	this novel hydrogel-electrospun composite	813:853	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	5	39	theme	different	987:995	arg1	applications					1016:1027	different tissue engineering applications	987:1027	different tissue engineering applications	987:1027	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	4	40	theme	favorable	721:729	arg1	cell					764:767	a favorable adipose-derived mesenchymal stem cell	719:767	a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment	719:787	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	4	40	theme	favorable	721:729	arg1	AMSC					772:775	AMSC	772:775	AMSC	772:775	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	4	41	theme	novel	818:822	arg1	composite					845:853	this novel hydrogel-electrospun composite	813:853	this novel hydrogel-electrospun composite	813:853	Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel hydrogel-electrospun composite.					
32718603	5	42	theme	tissue	997:1002	arg1	applications					1016:1027	different tissue engineering applications	987:1027	different tissue engineering applications	987:1027	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	2	43	theme	proposed	450:457	arg1	composite					459:467	the proposed composite	446:467	the proposed composite	446:467	The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis.					
32718603	5	44	theme	engineering	1004:1014	arg1	applications					1016:1027	different tissue engineering applications	987:1027	different tissue engineering applications	987:1027	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	1	45	theme	bilayer	150:156	arg1	scaffold					172:179	a bilayer nanocomposite scaffold	148:179	a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF)	148:247	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
32718603	0	46	theme	alginate/gelatin/silk	66:86	arg1	fibroin					88:94	oxidized alginate/gelatin/silk fibroin	57:94	oxidized alginate/gelatin/silk fibroin for tissue engineering application	57:129	A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application.					
32718603	5	47	with	structure	877:885	arg1	properties					906:915	its promising properties	892:915	its promising properties	892:915	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	5	47	with	structure	877:885	arg1	interaction					935:945	cell-material interaction	921:945	cell-material interaction	921:945	Such a nanocomposite structure with its promising properties and cell-material interaction may be considered as a new scaffold for different tissue engineering applications.					
32718603	1	48	theme	nanocomposite	158:170	arg1	scaffold					172:179	a bilayer nanocomposite scaffold	148:179	a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF)	148:247	In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods.					
34449122	0	0	theme	aryl	80:83	arg1	ketone					99:104	aryl ether nitrile ketone	80:104	aryl ether nitrile ketone	80:104	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	0	0	theme	aryl	80:83	arg1	Poly					75:78	Poly	75:78	Poly(aryl ether nitrile ketone) Substrates	75:116	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	2	1	contain	containing	290:299	arg1	Poly					258:261	Poly	258:261	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK)	258:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	1	contain	containing	290:299	arg1	alternative					341:351	a novel alternative	333:351	a novel alternative for its easy synthesis	333:374	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	1	contain	containing	290:299	arg2	PPENK					323:327	PPENK	323:327	PPENK	323:327	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	1	contain	containing	290:299	arg2	moiety					315:320	phthalazinone moiety	301:320	phthalazinone moiety (PPENK)	301:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	1	contain	containing	290:299	arg1	ketone					282:287	aryl ether nitrile ketone	263:287	aryl ether nitrile ketone	263:287	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	9	2	dep	cytocompatibility	1304:1320	arg1	the					1300:1302	the	1300:1302	the	1300:1302	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	1	3	theme	Bone-like	159:167	arg1	coating					192:198	a promising coating	180:198	a promising coating of poly(ether ether ketone) (PEEK) for bone implantation	180:255	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	1	3	theme	Bone-like	159:167	arg1	apatite					169:175	Bone-like apatite	159:175	Bone-like apatite	159:175	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	8	4	theme	apatite	1193:1199	arg1	quantity					1201:1208	a higher apatite quantity	1184:1208	a higher apatite quantity	1184:1208	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	3	5	theme	apatite	462:468	arg1	formation					449:457	the formation	445:457	the formation of apatite on the surface of PPENK substrate	445:502	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	0	6	theme	nitrile	91:97	arg1	ketone					99:104	aryl ether nitrile ketone	80:104	aryl ether nitrile ketone	80:104	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	0	6	theme	nitrile	91:97	arg1	Poly					75:78	Poly	75:78	Poly(aryl ether nitrile ketone) Substrates	75:116	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	5	7	from	crosslinking	757:768	arg1	substrate					844:852	PPENK substrate	838:852	PPENK substrate	838:852	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	3	8	theme	PPENK	488:492	arg1	substrate					494:502	PPENK substrate	488:502	PPENK substrate	488:502	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	9	9	theme	PPENK	1276:1280	arg1	substrate					1282:1290	PPENK substrate	1276:1290	PPENK substrate	1276:1290	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	0	10	theme	ether	85:89	arg1	ketone					99:104	aryl ether nitrile ketone	80:104	aryl ether nitrile ketone	80:104	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	0	10	theme	ether	85:89	arg1	Poly					75:78	Poly	75:78	Poly(aryl ether nitrile ketone) Substrates	75:116	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	3	11	theme	substrate	494:502	arg1	surface					477:483	the surface	473:483	the surface of PPENK substrate	473:502	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	1	12	theme	bone	239:242	arg1	implantation					244:255	bone implantation	239:255	bone implantation	239:255	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	4	13	theme	PPENK	662:666	arg1	substrate					668:676	PPENK substrate	662:676	PPENK substrate	662:676	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	5	14	theme	chitosan	806:813	arg1	methacrylate					815:826	chitosan methacrylate	806:826	chitosan methacrylate (CSMA) on PPENK substrate	806:852	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	5	14	theme	chitosan	806:813	arg1	CSMA					829:832	CSMA	829:832	CSMA	829:832	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	4	15	theme	allyl	604:608	arg1	PPENK-d					618:624	PPENK-d	618:624	PPENK-d	618:624	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	4	15	theme	allyl	604:608	arg1	groups					610:615	allyl groups	604:615	allyl groups (PPENK-d)	604:625	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	1	16	theme	promising	182:190	arg1	coating					192:198	a promising coating	180:198	a promising coating of poly(ether ether ketone) (PEEK) for bone implantation	180:255	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	1	16	theme	promising	182:190	arg1	apatite					169:175	Bone-like apatite	159:175	Bone-like apatite	159:175	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	3	17	theme	chitosan/gelatin	383:398	arg1	coating					416:422	chitosan/gelatin hybrid hydrogel coating	383:422	chitosan/gelatin hybrid hydrogel coating	383:422	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	6	18	theme	designed	924:931	arg1	structures					933:942	the designed structures	920:942	the designed structures	920:942	PPENK-d, GelMA, and CSMA are characterized by 1 H-NMR to confirm the designed structures.					
34449122	7	19	theme	chitosan	961:968	arg1	presence					949:956	The presence	945:956	The presence of chitosan	945:968	The presence of chitosan increases the chelation of calcium ions and thus induces the nucleation of apatite.					
34449122	5	20	theme	ultraviolet	745:755	arg1	crosslinking					757:768	the ultraviolet crosslinking	741:768	the ultraviolet crosslinking of gelatin methacrylate (GelMA)	741:800	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	8	21	theme	induced	1153:1159	arg1	coating					1169:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	3	22	theme	hybrid	400:405	arg1	coating					416:422	chitosan/gelatin hybrid hydrogel coating	383:422	chitosan/gelatin hybrid hydrogel coating	383:422	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	5	23	theme	methacrylate	781:792	arg1	methacrylate					815:826	chitosan methacrylate	806:826	chitosan methacrylate (CSMA) on PPENK substrate	806:852	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	5	23	theme	methacrylate	781:792	arg1	crosslinking					757:768	the ultraviolet crosslinking	741:768	the ultraviolet crosslinking of gelatin methacrylate (GelMA)	741:800	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	5	23	theme	methacrylate	781:792	arg1	CSMA					829:832	CSMA	829:832	CSMA	829:832	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	2	24	theme	novel	335:339	arg1	alternative					341:351	a novel alternative	333:351	a novel alternative for its easy synthesis	333:374	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	24	theme	novel	335:339	arg1	Poly					258:261	Poly	258:261	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK)	258:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	0	25	theme	Apatite	0:6	arg1	Formation					8:16	Apatite Formation	0:16	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating	0:61	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	8	26	theme	higher	1186:1191	arg1	quantity					1201:1208	a higher apatite quantity	1184:1208	a higher apatite quantity	1184:1208	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	2	27	theme	nitrile	274:280	arg1	Poly					258:261	Poly	258:261	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK)	258:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	27	theme	nitrile	274:280	arg1	ketone					282:287	aryl ether nitrile ketone	263:287	aryl ether nitrile ketone	263:287	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	8	28	theme	hydrogel	1234:1241	arg1	coating					1243:1249	the gelatin hydrogel coating	1222:1249	the gelatin hydrogel coating	1222:1249	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	5	29	theme	PPENK	838:842	arg1	substrate					844:852	PPENK substrate	838:852	PPENK substrate	838:852	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	8	30	theme	hydrogel	1136:1143	arg1	coating					1169:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	3	31	from	formation	449:457	arg1	surface					477:483	the surface	473:483	the surface of PPENK substrate	473:502	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	2	32	theme	ether	268:272	arg1	Poly					258:261	Poly	258:261	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK)	258:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	32	theme	ether	268:272	arg1	ketone					282:287	aryl ether nitrile ketone	263:287	aryl ether nitrile ketone	263:287	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	7	33	theme	apatite	1045:1051	arg1	nucleation					1031:1040	the nucleation	1027:1040	the nucleation of apatite	1027:1051	The presence of chitosan increases the chelation of calcium ions and thus induces the nucleation of apatite.					
34449122	7	34	theme	ions	1005:1008	arg1	chelation					984:992	the chelation	980:992	the chelation of calcium ions	980:1008	The presence of chitosan increases the chelation of calcium ions and thus induces the nucleation of apatite.					
34449122	2	35	theme	aryl	263:266	arg1	Poly					258:261	Poly	258:261	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK)	258:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	35	theme	aryl	263:266	arg1	ketone					282:287	aryl ether nitrile ketone	263:287	aryl ether nitrile ketone	263:287	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	1	36	theme	poly	203:206	arg1	coating					192:198	a promising coating	180:198	a promising coating of poly(ether ether ketone) (PEEK) for bone implantation	180:255	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	1	36	theme	poly	203:206	arg1	apatite					169:175	Bone-like apatite	159:175	Bone-like apatite	159:175	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	0	37	theme	Hydrogel	46:53	arg1	Coating					55:61	Chitosan/Gelatin Hydrogel Coating	29:61	Chitosan/Gelatin Hydrogel Coating	29:61	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	9	38	theme	MC3T3-E1	1342:1349	arg1	preosteoblasts					1351:1364	MC3T3-E1 preosteoblasts	1342:1364	MC3T3-E1 preosteoblasts	1342:1364	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	0	39	theme	Osteoblastic	129:140	arg1	Differentiation					142:156	Osteoblastic Differentiation	129:156	Osteoblastic Differentiation	129:156	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	3	40	theme	hydrogel	407:414	arg1	coating					416:422	chitosan/gelatin hybrid hydrogel coating	383:422	chitosan/gelatin hybrid hydrogel coating	383:422	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	2	41	theme	easy	361:364	arg1	synthesis					366:374	its easy synthesis	357:374	its easy synthesis	357:374	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	0	42	theme	Chitosan/Gelatin	29:44	arg1	Coating					55:61	Chitosan/Gelatin Hydrogel Coating	29:61	Chitosan/Gelatin Hydrogel Coating	29:61	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	9	43	theme	preosteoblasts	1351:1364	arg1	osteogenesis					1326:1337	osteogenesis	1326:1337	osteogenesis	1326:1337	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	9	43	theme	preosteoblasts	1351:1364	arg1	cytocompatibility					1304:1320	cytocompatibility	1304:1320	cytocompatibility	1304:1320	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	3	44	theme	osteogenic	566:575	arg1	property					577:584	its biocompatibility and osteogenic property	541:584	its biocompatibility and osteogenic property	541:584	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	5	45	from	methacrylate	815:826	arg1	substrate					844:852	PPENK substrate	838:852	PPENK substrate	838:852	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	1	46	theme	ether	208:212	arg1	poly					203:206	poly	203:206	poly(ether ether ketone) (PEEK)	203:233	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	1	46	theme	ether	208:212	arg1	ketone					220:225	ether ether ketone	208:225	ether ether ketone	208:225	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	8	47	theme	chitosan-containing	1116:1134	arg1	coating					1169:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	5	48	theme	gelatin	773:779	arg1	GelMA					795:799	GelMA	795:799	GelMA	795:799	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	5	48	theme	gelatin	773:779	arg1	methacrylate					781:792	gelatin methacrylate	773:792	gelatin methacrylate (GelMA)	773:800	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
34449122	7	49	theme	calcium	997:1003	arg1	ions					1005:1008	calcium ions	997:1008	calcium ions	997:1008	The presence of chitosan increases the chelation of calcium ions and thus induces the nucleation of apatite.					
34449122	3	50	theme	biocompatibility	545:560	arg1	property					577:584	its biocompatibility and osteogenic property	541:584	its biocompatibility and osteogenic property	541:584	Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property.					
34449122	2	51	theme	phthalazinone	301:313	arg1	PPENK					323:327	PPENK	323:327	PPENK	323:327	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	2	51	theme	phthalazinone	301:313	arg1	moiety					315:320	phthalazinone moiety	301:320	phthalazinone moiety (PPENK)	301:328	Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis.					
34449122	8	52	theme	gelatin	1226:1232	arg1	coating					1243:1249	the gelatin hydrogel coating	1222:1249	the gelatin hydrogel coating	1222:1249	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	1	53	theme	ether	214:218	arg1	poly					203:206	poly	203:206	poly(ether ether ketone) (PEEK)	203:233	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	1	53	theme	ether	214:218	arg1	ketone					220:225	ether ether ketone	208:225	ether ether ketone	208:225	Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation.					
34449122	8	54	theme	microstructural	1058:1072	arg1	results					1092:1098	The microstructural and compositional results	1054:1098	The microstructural and compositional results	1054:1098	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	4	55	contain	possessing	593:602	arg2	PPENK-d					618:624	PPENK-d	618:624	PPENK-d	618:624	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	4	55	contain	possessing	593:602	arg1	PPENK					587:591	PPENK	587:591	PPENK possessing allyl groups (PPENK-d)	587:625	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	4	55	contain	possessing	593:602	arg2	groups					610:615	allyl groups	604:615	allyl groups (PPENK-d)	604:625	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	4	56	theme	reactive	688:695	arg1	groups					697:702	reactive groups	688:702	reactive groups	688:702	PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups.					
34449122	0	57	theme	Poly	75:78	arg1	Substrates					107:116	Poly(aryl ether nitrile ketone) Substrates	75:116	Poly(aryl ether nitrile ketone) Substrates	75:116	Apatite Formation Induced by Chitosan/Gelatin Hydrogel Coating Anchored on Poly(aryl ether nitrile ketone) Substrates to Promote Osteoblastic Differentiation.					
34449122	9	58	from	coatings	1264:1271	arg1	substrate					1282:1290	PPENK substrate	1276:1290	PPENK substrate	1276:1290	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	8	59	theme	coating	1145:1151	arg1	coating					1169:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	8	60	theme	compositional	1078:1090	arg1	results					1092:1098	The microstructural and compositional results	1054:1098	The microstructural and compositional results	1054:1098	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	9	61	theme	apatite	1256:1262	arg1	coatings					1264:1271	The apatite coatings	1252:1271	The apatite coatings on PPENK substrate	1252:1290	The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.					
34449122	8	62	theme	apatite	1161:1167	arg1	coating					1169:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	the chitosan-containing hydrogel coating induced apatite coating	1112:1175	The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating.					
34449122	5	63	theme	hydrogel	709:716	arg1	coating					718:724	The hydrogel coating	705:724	The hydrogel coating	705:724	The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate.					
32246959	8	0	theme	growth	1220:1225	arg1	inhibition					1227:1236	growth inhibition	1220:1236	growth inhibition at a concentration of 1000 mg/L	1220:1268	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	1	1	theme	Eugenia	357:363	arg1	umbelliflora					365:376	Eugenia umbelliflora	357:376	Eugenia umbelliflora	357:376	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	8	2	theme	elongation	1119:1128	arg1	inhibition					1130:1139	seedling elongation inhibition	1110:1139	seedling elongation inhibition (SEI)	1110:1145	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	8	2	theme	elongation	1119:1128	arg1	SEI					1142:1144	SEI	1142:1144	SEI	1142:1144	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	7	3	theme	sativus	1071:1077	arg1	seeds					1079:1083	Cucumis sativus seeds	1063:1083	Cucumis sativus seeds	1063:1083	The potential phytotoxic effect of the nanocomposites was evaluated using Cucumis sativus seeds.					
32246959	1	4	theme	study	183:187	arg1	purpose					167:173	The purpose	163:173	The purpose of this study	163:187	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	10	5	theme	LC50	1477:1480	arg1	values					1482:1487	LC50 values	1477:1487	LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively	1477:1530	CMAgE and Eug showed cytotoxic effects against Artemia salina nauplii, with LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively, after 48 h.					
32246959	1	6	theme	umbelliflora	365:376	arg1	agent					392:396	a reducing agent	381:396	a reducing agent	381:396	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	1	6	theme	umbelliflora	365:376	arg1	extract					346:352	extract	346:352	extract of Eugenia umbelliflora	346:376	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	5	7	theme	minimum	736:742	arg1	16.5					860:863	16.5	860:863	16.5	860:863	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	5	7	theme	minimum	736:742	arg1	MIC					771:773	MIC	771:773	MIC	771:773	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	5	7	theme	minimum	736:742	arg1	concentrations					755:768	The minimum inhibitory concentrations	732:768	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans	732:853	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	10	8	theme	cytotoxic	1422:1430	arg1	effects					1432:1438	cytotoxic effects	1422:1438	cytotoxic effects	1422:1438	CMAgE and Eug showed cytotoxic effects against Artemia salina nauplii, with LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively, after 48 h.					
32246959	3	9	theme	antimicrobial	573:585	arg1	activity					587:594	The antimicrobial activity	569:594	The antimicrobial activity of the pathogenic microorganism	569:626	The antimicrobial activity of the pathogenic microorganism is reported here.					
32246959	4	10	theme	synthesized	650:660	arg1	nanoparticles					662:674	The synthesized nanoparticles	646:674	The synthesized nanoparticles	646:674	The synthesized nanoparticles were also characterized, and quantified by Ag analysis.					
32246959	0	11	theme	antimicrobial	126:138	arg1	nanoparticles					50:62	silver nanoparticles	43:62	silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity	43:160	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	11	theme	antimicrobial	126:138	arg1	activity					140:147	antimicrobial activity	126:147	antimicrobial activity	126:147	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	10	12	theme	Artemia	1448:1454	arg1	nauplii					1463:1469	Artemia salina nauplii	1448:1469	Artemia salina nauplii	1448:1469	CMAgE and Eug showed cytotoxic effects against Artemia salina nauplii, with LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively, after 48 h.					
32246959	5	13	theme	inhibitory	744:753	arg1	16.5					860:863	16.5	860:863	16.5	860:863	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	5	13	theme	inhibitory	744:753	arg1	MIC					771:773	MIC	771:773	MIC	771:773	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	5	13	theme	inhibitory	744:753	arg1	concentrations					755:768	The minimum inhibitory concentrations	732:768	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans	732:853	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	3	14	theme	microorganism	614:626	arg1	activity					587:594	The antimicrobial activity	569:594	The antimicrobial activity of the pathogenic microorganism	569:626	The antimicrobial activity of the pathogenic microorganism is reported here.					
32246959	7	15	theme	nanocomposites	1028:1041	arg1	effect					1014:1019	The potential phytotoxic effect	989:1019	The potential phytotoxic effect of the nanocomposites	989:1041	The potential phytotoxic effect of the nanocomposites was evaluated using Cucumis sativus seeds.					
32246959	10	16	theme	salina	1456:1461	arg1	nauplii					1463:1469	Artemia salina nauplii	1448:1469	Artemia salina nauplii	1448:1469	CMAgE and Eug showed cytotoxic effects against Artemia salina nauplii, with LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively, after 48 h.					
32246959	1	17	theme	reducing	383:390	arg1	agent					392:396	a reducing agent	381:396	a reducing agent	381:396	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	1	17	theme	reducing	383:390	arg1	extract					346:352	extract	346:352	extract of Eugenia umbelliflora	346:376	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	10	18	theme	and < 5.0 μL/mL	1503:1517	arg1	values					1482:1487	LC50 values	1477:1487	LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively	1477:1530	CMAgE and Eug showed cytotoxic effects against Artemia salina nauplii, with LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively, after 48 h.					
32246959	9	19	from	1000 mg/L	1323:1331	arg1	values					1298:1303	The germination index (GI) values	1271:1303	The germination index (GI) values	1271:1303	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	10	20	theme	72.5 μL/mL	1492:1501	arg1	and < 5.0 μL/mL					1503:1517	72.5 μL/mL and < 5.0 μL/mL	1492:1517	72.5 μL/mL and < 5.0 μL/mL respectively	1492:1530	CMAgE and Eug showed cytotoxic effects against Artemia salina nauplii, with LC50 values of 72.5 μL/mL and < 5.0 μL/mL respectively, after 48 h.					
32246959	8	21	theme	positive	1090:1097	arg1	values					1099:1104	The positive values	1086:1104	The positive values for seedling elongation inhibition (SEI)	1086:1145	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	3	22	theme	pathogenic	603:612	arg1	microorganism					614:626	the pathogenic microorganism	599:626	the pathogenic microorganism	599:626	The antimicrobial activity of the pathogenic microorganism is reported here.					
32246959	8	23	theme	1000 mg/L	1260:1268	arg1	concentration					1243:1255	a concentration	1241:1255	a concentration of 1000 mg/L	1241:1268	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	6	24	theme	synergistic	955:965	arg1	effect					967:972	significant synergistic effect	943:972	significant synergistic effect	943:972	The results show that these materials have significant synergistic effect on each other.					
32246959	9	25	theme	index	1287:1291	arg1	values					1298:1303	The germination index (GI) values	1271:1303	The germination index (GI) values	1271:1303	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	0	26	theme	Eugenia	0:6	arg1	umbelliflora					8:19	Eugenia umbelliflora	0:19	Eugenia umbelliflora	0:19	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	1	27	theme	silver	308:313	arg1	Ag					330:331	Ag NP	330:334	Ag NP	330:334	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	1	27	theme	silver	308:313	arg1	nanoparticles					315:327	silver nanoparticles	308:327	silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent	308:396	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	9	28	theme	%	1318:1318	arg1	values					1298:1303	The germination index (GI) values	1271:1303	The germination index (GI) values	1271:1303	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	7	29	theme	phytotoxic	1003:1012	arg1	effect					1014:1019	The potential phytotoxic effect	989:1019	The potential phytotoxic effect of the nanocomposites	989:1041	The potential phytotoxic effect of the nanocomposites was evaluated using Cucumis sativus seeds.					
32246959	9	30	theme	%	1310:1310	arg1	values					1298:1303	The germination index (GI) values	1271:1303	The germination index (GI) values	1271:1303	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	2	31	theme	Silver	399:404	arg1	nanoparticles					406:418	Silver nanoparticles	399:418	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	399:513	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite were synthesized using an extract of E. umbelliflora.					
32246959	5	32	theme	CMAgE	779:783	arg1	16.5					860:863	16.5	860:863	16.5	860:863	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	5	32	theme	CMAgE	779:783	arg1	MIC					771:773	MIC	771:773	MIC	771:773	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	5	32	theme	CMAgE	779:783	arg1	concentrations					755:768	The minimum inhibitory concentrations	732:768	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans	732:853	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	9	33	theme	GI	1294:1295	arg1	values					1298:1303	The germination index (GI) values	1271:1303	The germination index (GI) values	1271:1303	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	1	34	theme	magnetic	213:220	arg1	material					222:229	a new magnetic material	207:229	a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent	207:396	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	2	35	theme	CMAgE	498:502	arg1	composite					505:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite were synthesized using an extract of E. umbelliflora.					
32246959	6	36	theme	significant	943:953	arg1	effect					967:972	significant synergistic effect	943:972	significant synergistic effect	943:972	The results show that these materials have significant synergistic effect on each other.					
32246959	2	37	theme	O-carboxymethylchitosan/y-Fe2O3/Ag0	461:495	arg1	composite					505:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite were synthesized using an extract of E. umbelliflora.					
32246959	0	38	theme	silver	43:48	arg1	activity					140:147	antimicrobial activity	126:147	antimicrobial activity	126:147	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	38	theme	silver	43:48	arg1	toxicity					153:160	toxicity	153:160	toxicity	153:160	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	38	theme	silver	43:48	arg1	Synthesis					115:123	Synthesis	115:123	Synthesis	115:123	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	38	theme	silver	43:48	arg1	nanoparticles					50:62	silver nanoparticles	43:62	silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity	43:160	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	1	39	theme	new	209:211	arg1	material					222:229	a new magnetic material	207:229	a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent	207:396	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	2	40	theme	nanocomposite	447:459	arg1	composite					505:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite were synthesized using an extract of E. umbelliflora.					
32246959	1	41	with	material	222:229	arg1	properties					250:259	antimicrobial properties	236:259	antimicrobial properties	236:259	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	1	42	dep	properties	250:259	arg1	incorporated					262:273	incorporated	262:273	incorporated into a biopolymer	262:291	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	1	42	dep	properties	250:259	arg1	containing					297:306	containing	297:306	containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent	297:396	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	0	43	dep	nanoparticles	50:62	arg1	nanoparticles					50:62	silver nanoparticles	43:62	silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity	43:160	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	43	dep	nanoparticles	50:62	arg1	toxicity					153:160	toxicity	153:160	toxicity	153:160	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	43	dep	nanoparticles	50:62	arg1	Synthesis					115:123	Synthesis	115:123	Synthesis	115:123	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	0	43	dep	nanoparticles	50:62	arg1	activity					140:147	antimicrobial activity	126:147	antimicrobial activity	126:147	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	2	44	theme	magnetic	438:445	arg1	composite					505:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite	438:513	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite were synthesized using an extract of E. umbelliflora.					
32246959	9	45	theme	germination	1388:1398	arg1	inhibition					1374:1383	inhibition	1374:1383	inhibition of germination	1374:1398	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	5	46	theme	Candida	838:844	arg1	albicans					846:853	Candida albicans	838:853	Candida albicans	838:853	The minimum inhibitory concentrations (MIC) of CMAgE against Staphylococcus aureus, Escherichia coli, and Candida albicans were 16.5, 1000 and 500 μg/mL, respectively.					
32246959	0	47	theme	nanoparticles	50:62	arg1	reduction					30:38	reduction	30:38	reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity	30:160	Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity.					
32246959	8	48	from	concentration	1243:1255	arg1	inhibition					1227:1236	growth inhibition	1220:1236	growth inhibition at a concentration of 1000 mg/L	1220:1268	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	9	49	theme	germination	1275:1285	arg1	values					1298:1303	The germination index (GI) values	1271:1303	The germination index (GI) values	1271:1303	The germination index (GI) values of 40% and 80% at 1000 mg/L, for CMAgE and Eug, respectively, showed inhibition of germination.					
32246959	6	50	contain	have	938:941	arg1	materials					928:936	these materials	922:936	these materials	922:936	The results show that these materials have significant synergistic effect on each other.					
32246959	6	50	contain	have	938:941	arg2	effect					967:972	significant synergistic effect	943:972	significant synergistic effect	943:972	The results show that these materials have significant synergistic effect on each other.					
32246959	7	51	theme	potential	993:1001	arg1	effect					1014:1019	The potential phytotoxic effect	989:1019	The potential phytotoxic effect of the nanocomposites	989:1041	The potential phytotoxic effect of the nanocomposites was evaluated using Cucumis sativus seeds.					
32246959	8	52	theme	umbelliflora	1195:1206	arg1	CMAgE					1157:1161	CMAgE	1157:1161	CMAgE	1157:1161	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	8	52	theme	umbelliflora	1195:1206	arg1	extract					1176:1182	methanol extract	1167:1182	methanol extract of Eugenia umbelliflora (Eug)	1167:1212	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	1	53	theme	antimicrobial	236:248	arg1	properties					250:259	antimicrobial properties	236:259	antimicrobial properties	236:259	The purpose of this study was to synthesize a new magnetic material with antimicrobial properties, incorporated into a biopolymer and containing silver nanoparticles (Ag NP) prepared extract of Eugenia umbelliflora as a reducing agent.					
32246959	4	54	theme	Ag	719:720	arg1	analysis					722:729	Ag analysis	719:729	Ag analysis	719:729	The synthesized nanoparticles were also characterized, and quantified by Ag analysis.					
32246959	8	55	theme	seedling	1110:1117	arg1	inhibition					1130:1139	seedling elongation inhibition	1110:1139	seedling elongation inhibition (SEI)	1110:1145	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	8	55	theme	seedling	1110:1117	arg1	SEI					1142:1144	SEI	1142:1144	SEI	1142:1144	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
32246959	2	56	theme	umbelliflora	555:566	arg1	extract					541:547	an extract	538:547	an extract of E. umbelliflora	538:566	Silver nanoparticles incorporated into magnetic nanocomposite O-carboxymethylchitosan/y-Fe2O3/Ag0 (CMAgE) composite were synthesized using an extract of E. umbelliflora.					
32246959	7	57	theme	Cucumis	1063:1069	arg1	seeds					1079:1083	Cucumis sativus seeds	1063:1083	Cucumis sativus seeds	1063:1083	The potential phytotoxic effect of the nanocomposites was evaluated using Cucumis sativus seeds.					
32246959	8	58	theme	methanol	1167:1174	arg1	extract					1176:1182	methanol extract	1167:1182	methanol extract of Eugenia umbelliflora (Eug)	1167:1212	The positive values for seedling elongation inhibition (SEI) show that CMAgE and methanol extract of Eugenia umbelliflora (Eug) cause growth inhibition at a concentration of 1000 mg/L.					
34856214	4	0	dep	isotherm	770:777	arg1	the					755:757	the	755:757	the	755:757	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	1	1	theme	ionic	304:308	arg1	monomer					317:323	ionic liquid monomer	304:323	ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs)	304:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	2	2	theme	time	508:511	arg1	influence					487:495	the influence	483:495	the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior	483:585	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	5	3	theme	adsorption	955:964	arg1	process					966:972	The adsorption process	951:972	The adsorption process of CR on the surface of the adsorbent	951:1010	The adsorption process of CR on the surface of the adsorbent was endothermic and spontaneous.					
34856214	5	3	theme	adsorption	955:964	arg1	endothermic					1016:1026	endothermic	1016:1026	endothermic	1016:1026	The adsorption process of CR on the surface of the adsorbent was endothermic and spontaneous.					
34856214	4	4	theme	isotherm	770:777	arg1	results					744:750	The results	740:750	The results of the adsorption isotherm and adsorption kinetics	740:801	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	2	5	theme	red	419:421	arg1	removal					402:408	The adsorptive removal	387:408	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs	387:459	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	1	6	theme	liquid	310:315	arg1	monomer					317:323	ionic liquid monomer	304:323	ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs)	304:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	2	7	theme	contact	500:506	arg1	time					508:511	contact time	500:511	contact time	500:511	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	2	8	theme	temperature	552:562	arg1	influence					487:495	the influence	483:495	the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior	483:585	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	5	9	theme	CR	977:978	arg1	process					966:972	The adsorption process	951:972	The adsorption process of CR on the surface of the adsorbent	951:1010	The adsorption process of CR on the surface of the adsorbent was endothermic and spontaneous.					
34856214	5	9	theme	CR	977:978	arg1	endothermic					1016:1026	endothermic	1016:1026	endothermic	1016:1026	The adsorption process of CR on the surface of the adsorbent was endothermic and spontaneous.					
34856214	3	10	theme	IL	730:731	arg1	195.83 mg/g					717:727	195.83 mg/g	717:727	195.83 mg/g (IL@CNCs)	717:737	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	3	10	theme	IL	730:731	arg1	CNCs					733:736	IL@CNCs	730:736	IL@CNCs	730:736	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	1	11	theme	monomer	317:323	arg1	irradiation					280:290	direct irradiation	273:290	direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs)	273:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	8	12	theme	polymerization	1339:1352	arg1	parameters					1354:1363	polymerization parameters	1339:1363	polymerization parameters	1339:1363	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	0	13	theme	Gamma-ray	71:79	arg1	irradiation					81:91	Gamma-ray irradiation	71:91	Gamma-ray irradiation	71:91	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	3	14	theme	same	625:628	arg1	conditions					641:650	the same adsorption conditions	621:650	the same adsorption conditions	621:650	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	2	15	theme	concentrations	533:546	arg1	influence					487:495	the influence	483:495	the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior	483:585	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	4	16	theme	Freundlich	879:888	arg1	model					910:914	the Freundlich isotherm adsorption model	875:914	the Freundlich isotherm adsorption model	875:914	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	2	17	theme	aqueous	433:439	arg1	solution					441:448	aqueous solution	433:448	aqueous solution	433:448	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	3	18	theme	adsorption	630:639	arg1	conditions					641:650	the same adsorption conditions	621:650	the same adsorption conditions	621:650	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	2	19	theme	initial	525:531	arg1	concentrations					533:546	initial concentrations	525:546	initial concentrations	525:546	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	6	20	theme	aqueous	1054:1060	arg1	acidic					1075:1080	acidic	1075:1080	acidic	1075:1080	When the aqueous solution was acidic, it was more conducive to the adsorption of CR.					
34856214	6	20	theme	aqueous	1054:1060	arg1	solution					1062:1069	the aqueous solution	1050:1069	the aqueous solution	1050:1069	When the aqueous solution was acidic, it was more conducive to the adsorption of CR.					
34856214	2	21	theme	adsorptive	391:400	arg1	removal					402:408	The adsorptive removal	387:408	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs	387:459	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	1	22	theme	poly	335:338	arg1	CNCs					380:383	IL@CNCs	377:383	IL@CNCs	377:383	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	1	22	theme	poly	335:338	arg1	CNCs					371:374	poly (ionic liquids) functionalized CNCs	335:374	poly (ionic liquids) functionalized CNCs (IL@CNCs)	335:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	2	23	theme	values	517:522	arg1	influence					487:495	the influence	483:495	the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior	483:585	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	4	24	theme	adsorption	899:908	arg1	model					910:914	the Freundlich isotherm adsorption model	875:914	the Freundlich isotherm adsorption model	875:914	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	2	25	from	influence	487:495	arg1	behavior					578:585	adsorption behavior	567:585	adsorption behavior	567:585	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	8	26	dep	modified	1441:1448	arg1	liquids					1432:1438	ionic liquids	1426:1438	ionic liquids	1426:1438	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	2	27	theme	pH	514:515	arg1	values					517:522	pH values	514:522	pH values	514:522	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	7	28	theme	100	1133:1135	arg1	%					1136:1136	%	1136:1136	%	1136:1136	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	7	29	theme	adsorption	1165:1174	arg1	capacity					1176:1183	adsorption capacity	1165:1183	adsorption capacity	1165:1183	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	0	30	theme	Direct	0:5	arg1	grafting					7:14	Direct grafting	0:14	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.	0:143	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	2	31	from	solution	441:448	arg1	removal					402:408	The adsorptive removal	387:408	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs	387:459	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	2	32	theme	adsorption	567:576	arg1	behavior					578:585	adsorption behavior	567:585	adsorption behavior	567:585	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	7	33	theme	capacity	1176:1183	arg1	value					1156:1160	the value	1152:1160	the value of adsorption capacity	1152:1183	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	7	33	theme	capacity	1176:1183	arg1	199.95 mg/g					1188:1198	199.95 mg/g	1188:1198	199.95 mg/g	1188:1198	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	0	34	theme	cellulose	19:27	arg1	nanocrystals					29:40	cellulose nanocrystals	19:40	cellulose nanocrystals	19:40	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	1	35	theme	first	254:258	arg1	time					260:263	the first time	250:263	the first time	250:263	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	7	36	theme	coefficient	1227:1237	arg1	value					1208:1212	the value	1204:1212	the value of partition coefficient	1204:1237	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	7	36	theme	coefficient	1227:1237	arg1	9.64					1242:1245	9.64	1242:1245	9.64	1242:1245	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	1	37	theme	simple	161:166	arg1	method					182:187	a simple but effective method	159:187	a simple but effective method based on Gamma-ray initiated polymerization	159:231	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	4	38	theme	pseudo-second-order	924:942	arg1	model					944:948	the pseudo-second-order model	920:948	the pseudo-second-order model	920:948	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	5	39	theme	adsorbent	1002:1010	arg1	surface					987:993	the surface	983:993	the surface of the adsorbent	983:1010	The adsorption process of CR on the surface of the adsorbent was endothermic and spontaneous.					
34856214	4	40	theme	kinetics	794:801	arg1	results					744:750	The results	740:750	The results of the adsorption isotherm and adsorption kinetics	740:801	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	1	41	theme	ionic	341:345	arg1	poly					335:338	poly	335:338	poly (ionic liquids) functionalized CNCs (IL@CNCs)	335:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	1	41	theme	ionic	341:345	arg1	liquids					347:353	ionic liquids	341:353	ionic liquids	341:353	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	2	42	theme	@	455:455	arg1	CNCs					456:459	IL@CNCs	453:459	IL@CNCs	453:459	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	1	43	theme	effective	172:180	arg1	method					182:187	a simple but effective method	159:187	a simple but effective method based on Gamma-ray initiated polymerization	159:231	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	0	44	theme	nanocrystals	29:40	arg1	grafting					7:14	Direct grafting	0:14	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.	0:143	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	1	45	theme	direct	273:278	arg1	irradiation					280:290	direct irradiation	273:290	direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs)	273:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	0	46	theme	adsorptive	119:128	arg1	removal					130:136	adsorptive removal	119:136	adsorptive removal of CR	119:142	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	5	47	from	process	966:972	arg1	surface					987:993	the surface	983:993	the surface of the adsorbent	983:1010	The adsorption process of CR on the surface of the adsorbent was endothermic and spontaneous.					
34856214	4	48	theme	isotherm	890:897	arg1	model					910:914	the Freundlich isotherm adsorption model	875:914	the Freundlich isotherm adsorption model	875:914	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	8	49	theme	ionic	1426:1430	arg1	liquids					1432:1438	ionic liquids	1426:1438	ionic liquids	1426:1438	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	0	50	theme	CR	141:142	arg1	removal					130:136	adsorptive removal	119:136	adsorptive removal of CR	119:142	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	1	51	theme	functionalized	356:369	arg1	CNCs					380:383	IL@CNCs	377:383	IL@CNCs	377:383	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	1	51	theme	functionalized	356:369	arg1	CNCs					371:374	poly (ionic liquids) functionalized CNCs	335:374	poly (ionic liquids) functionalized CNCs (IL@CNCs)	335:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	7	52	theme	partition	1217:1225	arg1	coefficient					1227:1237	partition coefficient	1217:1237	partition coefficient	1217:1237	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	3	53	dep	increased	681:689	arg1	CNCs					708:711	CNCs	708:711	CNCs	708:711	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	3	54	theme	adsorption	657:666	arg1	capacity					668:675	the adsorption capacity	653:675	the adsorption capacity	653:675	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	2	55	theme	IL	453:454	arg1	CNCs					456:459	IL@CNCs	453:459	IL@CNCs	453:459	The adsorptive removal of Congo red (CR) from aqueous solution by IL@CNCs was also examined and the influence of contact time, pH values, initial concentrations and temperature on adsorption behavior was investigated in detail.					
34856214	4	56	theme	adsorption	759:768	arg1	isotherm					770:777	adsorption isotherm	759:777	adsorption isotherm	759:777	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	6	57	theme	CR	1126:1127	arg1	adsorption					1112:1121	the adsorption	1108:1121	the adsorption of CR	1108:1127	When the aqueous solution was acidic, it was more conducive to the adsorption of CR.					
34856214	0	58	with	grafting	7:14	arg1	utilization					103:113	their utilization	97:113	their utilization for adsorptive removal of CR	97:142	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	0	58	with	grafting	7:14	arg1	poly					47:50	poly	47:50	poly(ionic liquids) via Gamma-ray irradiation	47:91	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	0	58	with	grafting	7:14	arg1	liquids					58:64	ionic liquids	52:64	ionic liquids	52:64	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	7	59	from	breakthrough	1138:1149	arg1	value					1156:1160	the value	1152:1160	the value of adsorption capacity	1152:1183	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	7	59	from	breakthrough	1138:1149	arg1	199.95 mg/g					1188:1198	199.95 mg/g	1188:1198	199.95 mg/g	1188:1198	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	1	60	theme	CNCs	295:298	arg1	irradiation					280:290	direct irradiation	273:290	direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs)	273:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	4	61	theme	adsorption	783:792	arg1	kinetics					794:801	adsorption kinetics	783:801	adsorption kinetics	783:801	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	8	62	used	used	1393:1396	arg2	method					1374:1379	this method	1369:1379	this method	1369:1379	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	7	63	theme	%	1136:1136	arg1	breakthrough					1138:1149	100% breakthrough	1133:1149	100% breakthrough	1133:1149	At 100% breakthrough, the value of adsorption capacity is 199.95 mg/g and the value of partition coefficient is 9.64.					
34856214	1	64	theme	IL	377:378	arg1	CNCs					380:383	IL@CNCs	377:383	IL@CNCs	377:383	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	1	64	theme	IL	377:378	arg1	CNCs					371:374	poly (ionic liquids) functionalized CNCs	335:374	poly (ionic liquids) functionalized CNCs (IL@CNCs)	335:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	8	65	theme	adsorption	1262:1271	arg1	capacity					1273:1280	the adsorption capacity	1258:1280	the adsorption capacity	1258:1280	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	8	66	theme	parameters	1354:1363	arg1	adjustment					1325:1334	adjustment	1325:1334	adjustment of polymerization parameters	1325:1363	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	4	67	theme	experimental	819:830	arg1	data					832:835	the experimental data	815:835	the experimental data	815:835	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	4	67	theme	experimental	819:830	arg1	suitable					847:854	suitable	847:854	suitable	847:854	The results of the adsorption isotherm and adsorption kinetics showed that the experimental data were more suitable to be described by the Freundlich isotherm adsorption model and the pseudo-second-order model.					
34856214	3	68	theme	@	732:732	arg1	195.83 mg/g					717:727	195.83 mg/g	717:727	195.83 mg/g (IL@CNCs)	717:737	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	3	68	theme	@	732:732	arg1	CNCs					733:736	IL@CNCs	730:736	IL@CNCs	730:736	Under the same adsorption conditions, the adsorption capacity was increased from 59.72 mg/g (CNCs) to 195.83 mg/g (IL@CNCs).					
34856214	8	69	theme	modified	1441:1448	arg1	composites					1450:1459	preparation other poly (ionic liquids) modified composites	1402:1459	preparation other poly (ionic liquids) modified composites	1402:1459	Moreover, the adsorption capacity is expected to be further improved through adjustment of polymerization parameters and this method can also be used for preparation other poly (ionic liquids) modified composites.					
34856214	1	70	theme	@	379:379	arg1	CNCs					380:383	IL@CNCs	377:383	IL@CNCs	377:383	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	1	70	theme	@	379:379	arg1	CNCs					371:374	poly (ionic liquids) functionalized CNCs	335:374	poly (ionic liquids) functionalized CNCs (IL@CNCs)	335:384	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	1	71	theme	Gamma-ray	198:206	arg1	polymerization					218:231	Gamma-ray initiated polymerization	198:231	Gamma-ray initiated polymerization	198:231	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
34856214	0	72	theme	ionic	52:56	arg1	poly					47:50	poly	47:50	poly(ionic liquids) via Gamma-ray irradiation	47:91	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	0	72	theme	ionic	52:56	arg1	liquids					58:64	ionic liquids	52:64	ionic liquids	52:64	Direct grafting of cellulose nanocrystals with poly(ionic liquids) via Gamma-ray irradiation and their utilization for adsorptive removal of CR.					
34856214	1	73	theme	initiated	208:216	arg1	polymerization					218:231	Gamma-ray initiated polymerization	198:231	Gamma-ray initiated polymerization	198:231	In this work, a simple but effective method based on Gamma-ray initiated polymerization was reported for the first time through direct irradiation of CNCs and ionic liquid monomer to obtain poly (ionic liquids) functionalized CNCs (IL@CNCs).					
32512092	1	0	theme	starch	327:332	arg1	compatibility					298:310	the compatibility	294:310	the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA)	294:429	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	4	1	theme	storage	1189:1195	arg1	modulus					1197:1203	the storage modulus	1185:1203	the storage modulus	1185:1203	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	2	2	dep	transform	495:503	arg1	infrared					505:512	infrared	505:512	transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay	495:592	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	5	3	theme	Morphological	1232:1244	arg1	analysis					1246:1253	Morphological analysis	1232:1253	Morphological analysis	1232:1253	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	0	4	theme	lactic	58:63	arg1	poly					53:56	poly	53:56	poly(lactic acid)	53:69	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	0	4	theme	lactic	58:63	arg1	acid					65:68	lactic acid	58:68	lactic acid	58:68	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	5	5	theme	interface	1343:1351	arg1	compatibility					1353:1365	the interface compatibility	1339:1365	the interface compatibility of the nanocomposites	1339:1387	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	1	6	with	compatibility	298:310	arg1	acid					362:365	hydrophobic polylactic acid	339:365	hydrophobic polylactic acid (PLA)	339:371	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	6	with	compatibility	298:310	arg1	acetate-glycidylmethacrylate					392:419	ethylene-vinyl acetate-glycidylmethacrylate	377:419	ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA)	377:429	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	6	with	compatibility	298:310	arg1	EVA-GMA					422:428	EVA-GMA	422:428	EVA-GMA	422:428	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	6	with	compatibility	298:310	arg1	PLA					368:370	PLA	368:370	PLA	368:370	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	4	7	from	~22 °C	1071:1076	arg1	angle					1142:1146	contact angle	1134:1146	contact angle	1134:1146	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	7	from	~22 °C	1071:1076	arg1	temperature					1118:1128	the maximum decomposition temperature	1092:1128	the maximum decomposition temperature	1092:1128	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	1	8	theme	hydrophobic	339:349	arg1	acid					362:365	hydrophobic polylactic acid	339:365	hydrophobic polylactic acid (PLA)	339:371	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	8	theme	hydrophobic	339:349	arg1	PLA					368:370	PLA	368:370	PLA	368:370	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	3	9	theme	nanocomposites	883:896	arg1	size					871:874	the spherulites size	855:874	the spherulites size of the nanocomposites	855:896	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	4	10	theme	%	962:962	arg1	DDAB-GOQDs					964:973	0.25% DDAB-GOQDs	958:973	0.25% DDAB-GOQDs	958:973	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	1	11	theme	polylactic	351:360	arg1	acid					362:365	hydrophobic polylactic acid	339:365	hydrophobic polylactic acid (PLA)	339:371	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	11	theme	polylactic	351:360	arg1	PLA					368:370	PLA	368:370	PLA	368:370	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	5	12	theme	nanocomposites	1374:1387	arg1	compatibility					1353:1365	the interface compatibility	1339:1365	the interface compatibility of the nanocomposites	1339:1387	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	4	13	theme	obvious	982:988	arg1	enhancement					990:1000	obvious enhancement	982:1000	obvious enhancement in thermal stability and surface hydrophobicity	982:1048	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	5	14	dep	strong	1455:1460	arg1	polar					1462:1466	polar	1462:1466	polar	1462:1466	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	4	15	from	angle	1142:1146	arg1	increase					1059:1066	an increase	1056:1066	an increase	1056:1066	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	16	theme	~22 °C	1071:1076	arg1	increase					1059:1066	an increase	1056:1066	an increase	1056:1066	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	17	from	improvement	1170:1180	arg1	modulus					1197:1203	the storage modulus	1185:1203	the storage modulus	1185:1203	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	17	from	improvement	1170:1180	arg1	capacity					1222:1229	UV-shielding capacity	1209:1229	UV-shielding capacity	1209:1229	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	0	18	theme	ethylene-vinyl	75:88	arg1	acetate-glycidyl-methacrylate					90:118	ethylene-vinyl acetate-glycidyl-methacrylate	75:118	ethylene-vinyl acetate-glycidyl-methacrylate	75:118	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	3	19	theme	kinetics	713:720	arg1	analysis					722:729	iso-thermal crystallization kinetics analysis	685:729	iso-thermal crystallization kinetics analysis	685:729	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	5	20	theme	hydrogen	1468:1475	arg1	bond					1477:1480	strong polar hydrogen bond	1455:1480	strong polar hydrogen bond	1455:1480	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	5	21	theme	creep	1265:1269	arg1	experiments					1280:1290	creep behavior experiments	1265:1290	creep behavior experiments	1265:1290	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	6	22	theme	effective	1582:1590	arg1	DDAB-GOQDs					1555:1564	(0.25%) DDAB-GOQDs	1547:1564	(0.25%) DDAB-GOQDs	1547:1564	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	6	22	theme	effective	1582:1590	arg1	compatibilizer					1592:1605	an effective compatibilizer	1579:1605	an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance	1579:1731	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	4	23	theme	thermal	1005:1011	arg1	stability					1013:1021	thermal stability	1005:1021	thermal stability	1005:1021	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	5	24	theme	behavior	1271:1278	arg1	experiments					1280:1290	creep behavior experiments	1265:1290	creep behavior experiments	1265:1290	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	4	25	theme	similar	1162:1168	arg1	improvement					1170:1180	similar improvement	1162:1180	similar improvement in the storage modulus and UV-shielding capacity	1162:1229	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	26	theme	0.25	958:961	arg1	%					962:962	%	962:962	%	962:962	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	27	contain	containing	947:956	arg1	nanocomposites					932:945	the nanocomposites	928:945	the nanocomposites containing 0.25% DDAB-GOQDs	928:973	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	27	contain	containing	947:956	arg2	DDAB-GOQDs					964:973	0.25% DDAB-GOQDs	958:973	0.25% DDAB-GOQDs	958:973	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	2	28	theme	successful	436:445	arg1	synthesis					447:455	The successful synthesis	432:455	The successful synthesis of DDAB-GOQDs	432:469	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	1	29	theme	new	264:266	arg1	compatibilizer					268:281	a new compatibilizer	262:281	a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA)	262:429	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	29	theme	new	264:266	arg1	possibility					153:163	the possibility	149:163	the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs)	149:257	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	5	30	theme	polymeric	1516:1524	arg1	components					1526:1535	the polymeric components	1512:1535	the polymeric components	1512:1535	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	6	31	theme	thermal	1674:1680	arg1	stability					1682:1690	thermal stability	1674:1690	thermal stability	1674:1690	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	3	32	theme	0.25	743:746	arg1	%					747:747	%	747:747	%	747:747	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	6	33	theme	improved	1648:1655	arg1	crystallization					1657:1671	improved crystallization	1648:1671	improved crystallization	1648:1671	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	3	34	theme	polarized	640:648	arg1	microscopy					664:673	polarized light optical microscopy	640:673	polarized light optical microscopy (POM)	640:679	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	3	34	theme	polarized	640:648	arg1	POM					676:678	POM	676:678	POM	676:678	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	4	35	theme	contact	1134:1140	arg1	angle					1142:1146	contact angle	1134:1146	contact angle	1134:1146	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	36	theme	surface	1027:1033	arg1	hydrophobicity					1035:1048	surface hydrophobicity	1027:1048	surface hydrophobicity	1027:1048	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	3	37	theme	%	747:747	arg1	DDAB-GOQDs					749:758	0.25% DDAB-GOQDs	743:758	0.25% DDAB-GOQDs	743:758	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	2	38	theme	electron	548:555	arg1	microscopy					557:566	Transmission electron microscopy	535:566	Transmission electron microscopy (TEM)	535:572	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	2	38	theme	electron	548:555	arg1	TEM					569:571	TEM	569:571	TEM	569:571	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	3	39	theme	light	650:654	arg1	microscopy					664:673	polarized light optical microscopy	640:673	polarized light optical microscopy (POM)	640:679	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	3	39	theme	light	650:654	arg1	POM					676:678	POM	676:678	POM	676:678	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	4	40	theme	decomposition	1104:1116	arg1	temperature					1118:1128	the maximum decomposition temperature	1092:1128	the maximum decomposition temperature	1092:1128	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	2	41	theme	Transmission	535:546	arg1	microscopy					557:566	Transmission electron microscopy	535:566	Transmission electron microscopy (TEM)	535:572	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	2	41	theme	Transmission	535:546	arg1	TEM					569:571	TEM	569:571	TEM	569:571	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	1	42	theme	ethylene-vinyl	377:390	arg1	EVA-GMA					422:428	EVA-GMA	422:428	EVA-GMA	422:428	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	42	theme	ethylene-vinyl	377:390	arg1	acetate-glycidylmethacrylate					392:419	ethylene-vinyl acetate-glycidylmethacrylate	377:419	ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA)	377:429	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	3	43	theme	differential	599:610	arg1	calorimetry					621:631	The differential scanning calorimetry	595:631	The differential scanning calorimetry (DSC)	595:637	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	3	43	theme	differential	599:610	arg1	DSC					634:636	DSC	634:636	DSC	634:636	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	4	44	theme	UV-shielding	1209:1220	arg1	capacity					1222:1229	UV-shielding capacity	1209:1229	UV-shielding capacity	1209:1229	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	1	45	theme	-modified	208:216	arg1	DDAB-GOQDs					247:256	DDAB-GOQDs	247:256	DDAB-GOQDs	247:256	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	45	theme	-modified	208:216	arg1	dots					241:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots	168:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs)	168:257	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	3	46	theme	crystallization	793:807	arg1	rate					809:812	the crystallization rate	789:812	the crystallization rate	789:812	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	2	47	theme	Zeta-Plus	578:586	arg1	assay					588:592	Zeta-Plus assay	578:592	Zeta-Plus assay	578:592	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	4	48	from	increase	1059:1066	arg1	angle					1142:1146	contact angle	1134:1146	contact angle	1134:1146	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	48	from	increase	1059:1066	arg1	temperature					1118:1128	the maximum decomposition temperature	1092:1128	the maximum decomposition temperature	1092:1128	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	49	from	temperature	1118:1128	arg1	increase					1059:1066	an increase	1056:1066	an increase	1056:1066	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	0	50	theme	starch	41:46	arg1	compatibilization					16:32	compatibilization	16:32	compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate	16:118	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	4	51	theme	pure	911:914	arg1	composites					916:925	pure composites	911:925	pure composites	911:925	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	2	52	dep	Fourier	487:493	arg1	transform					495:503	transform	495:503	transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay	495:592	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	2	53	theme	DDAB-GOQDs	460:469	arg1	synthesis					447:455	The successful synthesis	432:455	The successful synthesis of DDAB-GOQDs	432:469	The successful synthesis of DDAB-GOQDs was verified by Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta-Plus assay.					
32512092	5	54	theme	strong	1455:1460	arg1	bond					1477:1480	strong polar hydrogen bond	1455:1480	strong polar hydrogen bond	1455:1480	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	3	55	theme	iso-thermal	685:695	arg1	analysis					722:729	iso-thermal crystallization kinetics analysis	685:729	iso-thermal crystallization kinetics analysis	685:729	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	4	56	theme	maximum	1096:1102	arg1	temperature					1118:1128	the maximum decomposition temperature	1092:1128	the maximum decomposition temperature	1092:1128	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	5	57	theme	electrostatic	1485:1497	arg1	force					1499:1503	electrostatic force	1485:1503	electrostatic force	1485:1503	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	6	58	theme	barrier	1693:1699	arg1	properties					1701:1710	barrier properties	1693:1710	barrier properties	1693:1710	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	5	59	theme	interpenetrated	1423:1437	arg1	network					1439:1445	the interpenetrated network	1419:1445	the interpenetrated network	1419:1445	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	6	60	theme	PLA/starch/EVA-GMA	1615:1632	arg1	system					1634:1639	the PLA/starch/EVA-GMA system	1611:1639	the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance	1611:1731	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	1	61	theme	graphene	218:225	arg1	DDAB-GOQDs					247:256	DDAB-GOQDs	247:256	DDAB-GOQDs	247:256	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	61	theme	graphene	218:225	arg1	dots					241:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots	168:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs)	168:257	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	3	62	theme	optical	656:662	arg1	microscopy					664:673	polarized light optical microscopy	640:673	polarized light optical microscopy (POM)	640:679	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	3	62	theme	optical	656:662	arg1	POM					676:678	POM	676:678	POM	676:678	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	5	63	theme	network	1439:1445	arg1	formation					1406:1414	the formation	1402:1414	the formation of the interpenetrated network	1402:1445	Morphological analysis, FTIR and creep behavior experiments revealed that adding 0.25% DDAB-GOQDs enhanced the interface compatibility of the nanocomposites by promoting the formation of the interpenetrated network through strong polar hydrogen bond or electrostatic force within the polymeric components.					
32512092	6	64	theme	due	1641:1643	arg1	system					1634:1639	the PLA/starch/EVA-GMA system	1611:1639	the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance	1611:1731	Overall, (0.25%) DDAB-GOQDs can serve as an effective compatibilizer for the PLA/starch/EVA-GMA system due to improved crystallization, thermal stability, barrier properties and creep-resistance.					
32512092	4	65	from	~24.1°	1082:1087	arg1	angle					1142:1146	contact angle	1134:1146	contact angle	1134:1146	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	65	from	~24.1°	1082:1087	arg1	temperature					1118:1128	the maximum decomposition temperature	1092:1128	the maximum decomposition temperature	1092:1128	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	1	66	theme	oxide	227:231	arg1	DDAB-GOQDs					247:256	DDAB-GOQDs	247:256	DDAB-GOQDs	247:256	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	66	theme	oxide	227:231	arg1	dots					241:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots	168:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs)	168:257	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	4	67	theme	~24.1°	1082:1087	arg1	increase					1059:1066	an increase	1056:1066	an increase	1056:1066	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	3	68	theme	scanning	612:619	arg1	calorimetry					621:631	The differential scanning calorimetry	595:631	The differential scanning calorimetry (DSC)	595:637	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	3	68	theme	scanning	612:619	arg1	DSC					634:636	DSC	634:636	DSC	634:636	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	0	69	with	starch	41:46	arg1	poly					53:56	poly	53:56	poly(lactic acid)	53:69	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	0	69	with	starch	41:46	arg1	acid					65:68	lactic acid	58:68	lactic acid	58:68	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	0	69	with	starch	41:46	arg1	acetate-glycidyl-methacrylate					90:118	ethylene-vinyl acetate-glycidyl-methacrylate	75:118	ethylene-vinyl acetate-glycidyl-methacrylate	75:118	An approach for compatibilization of the starch with poly(lactic acid) and ethylene-vinyl acetate-glycidyl-methacrylate.					
32512092	3	70	theme	spherulites	859:869	arg1	size					871:874	the spherulites size	855:874	the spherulites size of the nanocomposites	855:896	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	1	71	theme	quantum	233:239	arg1	DDAB-GOQDs					247:256	DDAB-GOQDs	247:256	DDAB-GOQDs	247:256	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	71	theme	quantum	233:239	arg1	dots					241:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots	168:244	dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs)	168:257	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	72	theme	hydrophilic	315:325	arg1	starch					327:332	hydrophilic starch	315:332	hydrophilic starch	315:332	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	4	73	from	enhancement	990:1000	arg1	hydrophobicity					1035:1048	surface hydrophobicity	1027:1048	surface hydrophobicity	1027:1048	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	4	73	from	enhancement	990:1000	arg1	stability					1013:1021	thermal stability	1005:1021	thermal stability	1005:1021	Compared to pure composites, the nanocomposites containing 0.25% DDAB-GOQDs showed obvious enhancement in thermal stability and surface hydrophobicity, with an increase of ~22 °C and ~24.1° in the maximum decomposition temperature and contact angle, coupled with similar improvement in the storage modulus and UV-shielding capacity.					
32512092	3	74	theme	crystallization	697:711	arg1	analysis					722:729	iso-thermal crystallization kinetics analysis	685:729	iso-thermal crystallization kinetics analysis	685:729	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	3	75	theme	nucleation	818:827	arg1	density					829:835	nucleation density	818:835	nucleation density	818:835	The differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and iso-thermal crystallization kinetics analysis showed that 0.25% DDAB-GOQDs could significantly increase the crystallization rate and nucleation density as well as reduce the spherulites size of the nanocomposites.					
32512092	1	76	theme	dots	241:244	arg1	compatibilizer					268:281	a new compatibilizer	262:281	a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA)	262:429	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
32512092	1	76	theme	dots	241:244	arg1	possibility					153:163	the possibility	149:163	the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs)	149:257	In this study, we evaluated the possibility of dimethyldidodecylammonium bromide (DDAB)-modified graphene oxide quantum dots (DDAB-GOQDs) as a new compatibilizer to improve the compatibility of hydrophilic starch with hydrophobic polylactic acid (PLA) and ethylene-vinyl acetate-glycidylmethacrylate (EVA-GMA).					
35515389	9	0	theme	O-glycans	1576:1584	arg1	abundance					1508:1516	the relative abundance	1495:1516	the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans	1495:1584	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	7	1	from	HVs	1257:1259	arg1	cohorts					1294:1300	both discovery and validation cohorts	1264:1300	both discovery and validation cohorts	1264:1300	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	2	2	theme	pandemic	333:340	arg1	status					314:319	the status	310:319	the status of a global pandemic	310:340	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	11	3	theme	complex	1900:1906	arg1	changes					1922:1928	the complex physiological changes	1896:1928	the complex physiological changes in the T2DM patients	1896:1949	Our findings provide useful information to understand the complex physiological changes in the T2DM patients.					
35515389	6	4	theme	T2DM	1109:1112	arg1	patients					1114:1121	T2DM patients	1109:1121	T2DM patients	1109:1121	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	10	5	theme	LacNAc	1692:1697	arg1	glycopattern					1676:1687	the glycopattern	1672:1687	the glycopattern of LacNAc	1672:1697	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	2	6	theme	diabetes	272:279	arg1	T2DM					291:294	T2DM	291:294	T2DM	291:294	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	2	6	theme	diabetes	272:279	arg1	mellitus					281:288	Type 2 diabetes mellitus	265:288	Type 2 diabetes mellitus (T2DM)	265:295	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	10	7	theme	salivary	1715:1722	arg1	glycoproteins					1724:1736	salivary glycoproteins	1715:1736	salivary glycoproteins from T2DM patients	1715:1755	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	5	8	theme	lectin-mediated	816:830	arg1	capture					841:847	lectin-mediated affinity capture	816:847	lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS	816:885	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	9	9	theme	T2DM	1628:1631	arg1	patients					1633:1640	T2DM patients	1628:1640	T2DM patients	1628:1640	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	6	10	theme	significant	1069:1079	arg1	alteration					1081:1090	significant alteration	1069:1090	significant alteration in the saliva of T2DM patients	1069:1121	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	6	10	theme	significant	1069:1079	arg1	result					976:981	a result	974:981	a result	974:981	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	0	11	theme	diabetes	122:129	arg1	mellitus					131:138	type 2 diabetes mellitus	115:138	type 2 diabetes mellitus	115:138	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	3	12	theme	present	487:493	arg1	study					495:499	the present study	483:499	the present study	483:499	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	13	dep	HVs	578:580	arg1	n					583:583	n = 80	583:588	n = 80	583:588	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	9	14	from	increase	1596:1603	arg1	glycopattern					1612:1623	the glycopattern	1608:1623	the glycopattern in T2DM patients	1608:1640	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	0	15	from	alterations	66:76	arg1	proteins					90:97	salivary proteins	81:97	salivary proteins	81:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	7	16	theme	validation	1283:1292	arg1	cohorts					1294:1300	both discovery and validation cohorts	1264:1300	both discovery and validation cohorts	1264:1300	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	11	17	theme	physiological	1908:1920	arg1	changes					1922:1928	the complex physiological changes	1896:1928	the complex physiological changes in the T2DM patients	1896:1949	Our findings provide useful information to understand the complex physiological changes in the T2DM patients.					
35515389	5	18	theme	MALDI-TOF/TOF-MS	870:885	arg1	capture					841:847	lectin-mediated affinity capture	816:847	lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS	816:885	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	5	19	gly	glycoproteins	852:864	arg1	glycoproteins					852:864	glycoproteins	852:864	glycoproteins	852:864	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	7	20	theme	discovery	1269:1277	arg1	cohorts					1294:1300	both discovery and validation cohorts	1264:1300	both discovery and validation cohorts	1264:1300	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	2	21	theme	Type	265:268	arg1	T2DM					291:294	T2DM	291:294	T2DM	291:294	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	2	21	theme	Type	265:268	arg1	mellitus					281:288	Type 2 diabetes mellitus	265:288	Type 2 diabetes mellitus (T2DM)	265:295	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	7	22	theme	Galβ-1,4GlcNAc	1153:1166	arg1	glycopattern					1137:1148	the glycopattern	1133:1148	the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL	1133:1193	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	11	23	theme	T2DM	1937:1940	arg1	patients					1942:1949	the T2DM patients	1933:1949	the T2DM patients	1933:1949	Our findings provide useful information to understand the complex physiological changes in the T2DM patients.					
35515389	3	24	theme	=	548:548	arg1	T2DM					540:543	T2DM	540:543	T2DM (n = 72)	540:552	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	24	theme	=	548:548	arg1	n					546:546	n = 72	546:551	n = 72	546:551	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	25	dep	discovery	622:630	arg1	cohorts					647:653	cohorts	647:653	cohorts	647:653	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	0	26	theme	salivary	81:88	arg1	proteins					90:97	salivary proteins	81:97	salivary proteins	81:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	1	27	theme	several	170:176	arg1	processes					189:197	several biological processes	170:197	several biological processes	170:197	Glycosylation is involved in several biological processes, and its alterations can reflect the process of certain diseases.					
35515389	3	28	from	patients	526:533	arg1	specimens					511:519	salivary specimens	502:519	salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80)	502:589	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	2	29	theme	salivary	370:377	arg1	glycosylation					387:399	salivary protein glycosylation	370:399	salivary protein glycosylation	370:399	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	0	30	theme	type	115:118	arg1	mellitus					131:138	type 2 diabetes mellitus	115:138	type 2 diabetes mellitus	115:138	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	0	31	theme	Integrated	0:9	arg1	strategy					21:28	Integrated glycomics strategy	0:28	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins	0:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	4	32	theme	lectin	679:684	arg1	microarray					686:695	lectin microarray	679:695	lectin microarray	679:695	A method combining the lectin microarray and lectin blotting was employed to investigate and confirm the altered glycopatterns in salivary glycoproteins.					
35515389	2	33	theme	healthy	409:415	arg1	subjects					417:424	healthy subjects	409:424	healthy subjects	409:424	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	8	34	dep	m/z	1403:1405	arg1	e.g.					1398:1401	e.g.	1398:1401	e.g.	1398:1401	The MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients.					
35515389	9	35	theme	relative	1499:1506	arg1	abundance					1508:1516	the relative abundance	1495:1516	the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans	1495:1584	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	7	36	theme	T2DM	1231:1234	arg1	patients					1236:1243	T2DM patients	1231:1243	T2DM patients	1231:1243	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	6	37	from	alteration	1081:1090	arg1	saliva					1099:1104	the saliva	1095:1104	the saliva of T2DM patients	1095:1121	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	2	38	from	difference	356:365	arg1	glycosylation					387:399	salivary protein glycosylation	370:399	salivary protein glycosylation	370:399	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	5	39	theme	affinity	832:839	arg1	capture					841:847	lectin-mediated affinity capture	816:847	lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS	816:885	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	0	40	theme	alterations	66:76	arg1	evaluation					38:47	the evaluation	34:47	the evaluation of glycosylation alterations in salivary proteins	34:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	4	41	theme	altered	761:767	arg1	glycopatterns					769:781	the altered glycopatterns	757:781	the altered glycopatterns in salivary glycoproteins	757:807	A method combining the lectin microarray and lectin blotting was employed to investigate and confirm the altered glycopatterns in salivary glycoproteins.					
35515389	2	42	theme	global	326:331	arg1	pandemic					333:340	a global pandemic	324:340	a global pandemic	324:340	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	3	43	with	patients	526:533	arg1	volunteers					566:575	healthy volunteers	558:575	healthy volunteers (HVs, n = 80)	558:589	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	43	with	patients	526:533	arg1	HVs					578:580	HVs	578:580	HVs	578:580	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	43	with	patients	526:533	arg1	n					546:546	n = 72	546:551	n = 72	546:551	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	43	with	patients	526:533	arg1	T2DM					540:543	T2DM	540:543	T2DM (n = 72)	540:552	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	9	44	theme	LacNAc-containing	1523:1539	arg1	N-glycans					1541:1549	3 LacNAc-containing N-glycans	1521:1549	3 LacNAc-containing N-glycans	1521:1549	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	5	45	theme	glycans	962:968	arg1	information					935:945	the precise structural information	912:945	the precise structural information of the altered glycans	912:968	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	9	46	theme	LacNAc-containing	1558:1574	arg1	O-glycans					1576:1584	10 LacNAc-containing O-glycans	1555:1584	10 LacNAc-containing O-glycans	1555:1584	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	1	47	theme	diseases	255:262	arg1	process					236:242	the process	232:242	the process of certain diseases	232:262	Glycosylation is involved in several biological processes, and its alterations can reflect the process of certain diseases.					
35515389	10	48	theme	LacNAc-containing	1777:1793	arg1	N/O-glycans					1795:1805	LacNAc-containing N/O-glycans	1777:1805	LacNAc-containing N/O-glycans	1777:1805	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	3	49	theme	salivary	502:509	arg1	specimens					511:519	salivary specimens	502:519	salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80)	502:589	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	1	50	theme	biological	178:187	arg1	processes					189:197	several biological processes	170:197	several biological processes	170:197	Glycosylation is involved in several biological processes, and its alterations can reflect the process of certain diseases.					
35515389	9	51	from	glycopattern	1612:1623	arg1	patients					1633:1640	T2DM patients	1628:1640	T2DM patients	1628:1640	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	4	52	theme	salivary	786:793	arg1	glycoproteins					795:807	salivary glycoproteins	786:807	salivary glycoproteins	786:807	A method combining the lectin microarray and lectin blotting was employed to investigate and confirm the altered glycopatterns in salivary glycoproteins.					
35515389	0	53	from	proteins	90:97	arg1	evaluation					38:47	the evaluation	34:47	the evaluation of glycosylation alterations in salivary proteins	34:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	5	54	theme	glycoproteins	852:864	arg1	capture					841:847	lectin-mediated affinity capture	816:847	lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS	816:885	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	11	55	from	changes	1922:1928	arg1	patients					1942:1949	the T2DM patients	1933:1949	the T2DM patients	1933:1949	Our findings provide useful information to understand the complex physiological changes in the T2DM patients.					
35515389	1	56	gly	Glycosylation	141:153	arg1	processes					189:197	several biological processes	170:197	several biological processes	170:197	Glycosylation is involved in several biological processes, and its alterations can reflect the process of certain diseases.					
35515389	10	57	theme	T2DM	1743:1746	arg1	patients					1748:1755	T2DM patients	1743:1755	T2DM patients	1743:1755	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	2	58	with	subjects	417:424	arg1	T2DM					444:447	T2DM	444:447	T2DM	444:447	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	5	59	theme	altered	954:960	arg1	glycans					962:968	the altered glycans	950:968	the altered glycans	950:968	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	2	60	with	patients	430:437	arg1	T2DM					444:447	T2DM	444:447	T2DM	444:447	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	3	61	theme	healthy	558:564	arg1	volunteers					566:575	healthy volunteers	558:575	healthy volunteers (HVs, n = 80)	558:589	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	3	61	theme	healthy	558:564	arg1	HVs					578:580	HVs	578:580	HVs	578:580	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	5	62	theme	precise	916:922	arg1	information					935:945	the precise structural information	912:945	the precise structural information of the altered glycans	912:968	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	6	63	theme	patients	1114:1121	arg1	saliva					1099:1104	the saliva	1095:1104	the saliva of T2DM patients	1095:1121	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	8	64	theme	LacNAc-containing	1367:1383	arg1	N/O-glycans					1385:1395	10 and 7 LacNAc-containing N/O-glycans	1358:1395	10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients	1358:1483	The MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients.					
35515389	5	65	theme	structural	924:933	arg1	information					935:945	the precise structural information	912:945	the precise structural information of the altered glycans	912:968	Then, lectin-mediated affinity capture of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise structural information of the altered glycans.					
35515389	0	66	from	evaluation	38:47	arg1	proteins					90:97	salivary proteins	81:97	salivary proteins	81:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	4	67	theme	lectin	701:706	arg1	blotting					708:715	lectin blotting	701:715	lectin blotting	701:715	A method combining the lectin microarray and lectin blotting was employed to investigate and confirm the altered glycopatterns in salivary glycoproteins.					
35515389	11	68	theme	useful	1863:1868	arg1	information					1870:1880	useful information	1863:1880	useful information to understand the complex physiological changes in the T2DM patients	1863:1949	Our findings provide useful information to understand the complex physiological changes in the T2DM patients.					
35515389	0	69	theme	glycomics	11:19	arg1	strategy					21:28	Integrated glycomics strategy	0:28	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins	0:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	4	70	from	glycopatterns	769:781	arg1	glycoproteins					795:807	salivary glycoproteins	786:807	salivary glycoproteins	786:807	A method combining the lectin microarray and lectin blotting was employed to investigate and confirm the altered glycopatterns in salivary glycoproteins.					
35515389	10	71	from	patients	1748:1755	arg1	glycoproteins					1724:1736	salivary glycoproteins	1715:1736	salivary glycoproteins from T2DM patients	1715:1755	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	8	72	theme	T2DM	1471:1474	arg1	patients					1476:1483	T2DM patients	1471:1483	T2DM patients	1471:1483	The MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients.					
35515389	6	73	dep	lectins	1018:1024	arg1	DSA					1057:1059	DSA	1057:1059	DSA	1057:1059	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	6	73	dep	lectins	1018:1024	arg1	VVA					1032:1034	VVA	1032:1034	VVA	1032:1034	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	6	73	dep	lectins	1018:1024	arg1	Jacalin					1037:1043	Jacalin	1037:1043	Jacalin	1037:1043	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	6	73	dep	lectins	1018:1024	arg1	lectins					1018:1024	5 lectins	1016:1024	5 lectins (LEL, VVA, Jacalin, RCA120 and DSA)	1016:1060	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	6	73	dep	lectins	1018:1024	arg1	LEL					1027:1029	LEL	1027:1029	LEL	1027:1029	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	6	73	dep	lectins	1018:1024	arg1	RCA120					1046:1051	RCA120	1046:1051	RCA120	1046:1051	As a result, the glycopatterns recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant alteration in the saliva of T2DM patients.					
35515389	10	74	gly	glycoproteins	1724:1736	arg1	glycoproteins					1724:1736	salivary glycoproteins	1715:1736	salivary glycoproteins from T2DM patients	1715:1755	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	3	75	theme	=	585:585	arg1	n					583:583	n = 80	583:588	n = 80	583:588	In the present study, salivary specimens from patients with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided into discovery and validation cohorts.					
35515389	10	76	from	increase	1765:1772	arg1	N/O-glycans					1795:1805	LacNAc-containing N/O-glycans	1777:1805	LacNAc-containing N/O-glycans	1777:1805	These results indicated that the glycopattern of LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase in LacNAc-containing N/O-glycans may contribute to this alteration.					
35515389	7	77	theme	significant	1207:1217	arg1	increase					1219:1226	a significant increase	1205:1226	a significant increase in T2DM patients	1205:1243	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	8	78	theme	MALDI-TOF/TOF-MS	1307:1322	arg1	results					1324:1330	The MALDI-TOF/TOF-MS results	1303:1330	The MALDI-TOF/TOF-MS results	1303:1330	The MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients.					
35515389	2	79	theme	protein	379:385	arg1	glycosylation					387:399	salivary protein glycosylation	370:399	salivary protein glycosylation	370:399	Type 2 diabetes mellitus (T2DM) has attained the status of a global pandemic; however, the difference in salivary protein glycosylation between healthy subjects and patients with T2DM has not been fully understood.					
35515389	0	80	theme	glycosylation	52:64	arg1	alterations					66:76	glycosylation alterations	52:76	glycosylation alterations in salivary proteins	52:97	Integrated glycomics strategy for the evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus.					
35515389	8	81	dep	N/O-glycans	1385:1395	arg1	1562.470					1432:1439	1562.470	1432:1439	1562.470	1432:1439	The MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients.					
35515389	8	81	dep	N/O-glycans	1385:1395	arg1	11 688.613					1417:1426	11 688.613	1417:1426	11 688.613	1417:1426	The MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified only in T2DM patients.					
35515389	7	82	from	increase	1219:1226	arg1	patients					1236:1243	T2DM patients	1231:1243	T2DM patients	1231:1243	Notably, the glycopattern of Galβ-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in T2DM patients compared to HVs in both discovery and validation cohorts.					
35515389	9	83	theme	N-glycans	1541:1549	arg1	abundance					1508:1516	the relative abundance	1495:1516	the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans	1495:1584	Besides, the relative abundance of 3 LacNAc-containing N-glycans and 10 LacNAc-containing O-glycans showed an increase in the glycopattern in T2DM patients.					
35515389	4	84	gly	glycoproteins	795:807	arg1	glycoproteins					795:807	salivary glycoproteins	786:807	salivary glycoproteins	786:807	A method combining the lectin microarray and lectin blotting was employed to investigate and confirm the altered glycopatterns in salivary glycoproteins.					
35515389	1	85	theme	certain	247:253	arg1	diseases					255:262	certain diseases	247:262	certain diseases	247:262	Glycosylation is involved in several biological processes, and its alterations can reflect the process of certain diseases.					
33799264	6	0	theme	NaCas	1024:1028	arg1	ability					1000:1006	the ability	996:1006	the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin	996:1191	These results were attributed to the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin.					
33799264	2	1	theme	nanoparticles	397:409	arg1	properties					331:340	physicochemical properties	315:340	physicochemical properties	315:340	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	2	1	theme	nanoparticles	397:409	arg1	characteristics					363:377	curcumin release characteristics	346:377	curcumin release characteristics	346:377	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	6	2	theme	steric	1098:1103	arg1	repulsion					1123:1131	the steric and electrostatic repulsion	1094:1131	the steric and electrostatic repulsion between them	1094:1144	These results were attributed to the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin.					
33799264	0	3	theme	Physicochemical	86:100	arg1	properties					114:123	Physicochemical and release properties	86:123	properties	114:123	Curcumin-loaded core-shell biopolymer nanoparticles produced by the pH-driven method: Physicochemical and release properties.					
33799264	3	4	dep	Fourier	425:431	arg1	transform					433:441	transform	433:441	transform infrared spectroscopy and X-ray diffraction analyses	433:494	Fourier transform infrared spectroscopy and X-ray diffraction analyses indicated that curcumin was encapsulated in an amorphous state inside the nanoparticles.					
33799264	2	5	theme	core-shell	386:395	arg1	nanoparticles					397:409	the core-shell nanoparticles	382:409	the core-shell nanoparticles	382:409	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	4	6	theme	Particle	585:592	arg1	size					594:597	Particle size	585:597	Particle size	585:597	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	3	7	dep	transform	433:441	arg1	infrared					443:450	infrared	443:450	transform infrared spectroscopy and X-ray diffraction analyses	433:494	Fourier transform infrared spectroscopy and X-ray diffraction analyses indicated that curcumin was encapsulated in an amorphous state inside the nanoparticles.					
33799264	0	8	theme	release	106:112	arg1	properties					114:123	Physicochemical and release properties	86:123	properties	114:123	Curcumin-loaded core-shell biopolymer nanoparticles produced by the pH-driven method: Physicochemical and release properties.					
33799264	6	9	theme	curcumin	1184:1191	arg1	release					1173:1179	the release	1169:1179	the release of curcumin	1169:1191	These results were attributed to the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin.					
33799264	2	10	theme	release	355:361	arg1	characteristics					363:377	curcumin release characteristics	346:377	curcumin release characteristics	346:377	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	5	11	theme	curcumin	830:837	arg1	activity					814:821	The DPPH radical scavenging activity	786:821	The DPPH radical scavenging activity of the curcumin	786:837	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	2	12	theme	composition	296:306	arg1	influences					270:279	The influences	266:279	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles	266:409	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	2	13	theme	coating	288:294	arg1	composition					296:306	the coating composition	284:306	the coating composition	284:306	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	0	14	theme	core-shell	16:25	arg1	biopolymer					27:36	Curcumin-loaded core-shell biopolymer	0:36	Curcumin-loaded core-shell biopolymer	0:36	Curcumin-loaded core-shell biopolymer nanoparticles produced by the pH-driven method: Physicochemical and release properties.					
33799264	2	15	theme	curcumin	346:353	arg1	characteristics					363:377	curcumin release characteristics	346:377	curcumin release characteristics	346:377	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	0	16	theme	Curcumin-loaded	0:14	arg1	biopolymer					27:36	Curcumin-loaded core-shell biopolymer	0:36	Curcumin-loaded core-shell biopolymer	0:36	Curcumin-loaded core-shell biopolymer nanoparticles produced by the pH-driven method: Physicochemical and release properties.					
33799264	2	17	from	influences	270:279	arg1	properties					331:340	physicochemical properties	315:340	physicochemical properties	315:340	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	2	17	from	influences	270:279	arg1	characteristics					363:377	curcumin release characteristics	346:377	curcumin release characteristics	346:377	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	4	18	theme	pH	765:766	arg1	changes					768:774	pH changes	765:774	pH changes	765:774	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	5	19	theme	gastrointestinal	911:926	arg1	prolonged					952:960	prolonged	952:960	prolonged	952:960	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	5	19	theme	gastrointestinal	911:926	arg1	release					928:934	the gastrointestinal release	907:934	the gastrointestinal release of curcumin	907:946	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	5	20	theme	DPPH	790:793	arg1	activity					814:821	The DPPH radical scavenging activity	786:821	The DPPH radical scavenging activity of the curcumin	786:837	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	4	21	theme	term	742:745	arg1	storage					747:753	long term storage	737:753	long term storage	737:753	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	21	theme	term	742:745	arg1	conditions					725:734	different environmental conditions	701:734	different environmental conditions: long term storage, heating, pH changes and salt	701:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	5	22	theme	radical	795:801	arg1	activity					814:821	The DPPH radical scavenging activity	786:821	The DPPH radical scavenging activity of the curcumin	786:837	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	2	23	theme	physicochemical	315:329	arg1	properties					331:340	physicochemical properties	315:340	physicochemical properties	315:340	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	4	24	theme	long	737:740	arg1	storage					747:753	long term storage	737:753	long term storage	737:753	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	24	theme	long	737:740	arg1	conditions					725:734	different environmental conditions	701:734	different environmental conditions: long term storage, heating, pH changes and salt	701:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	3	25	theme	X-ray	469:473	arg1	analyses					487:494	X-ray diffraction analyses	469:494	X-ray diffraction analyses	469:494	Fourier transform infrared spectroscopy and X-ray diffraction analyses indicated that curcumin was encapsulated in an amorphous state inside the nanoparticles.					
33799264	4	26	theme	biopolymer	647:656	arg1	stable					688:693	stable	688:693	stable	688:693	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	26	theme	biopolymer	647:656	arg1	nanoparticles					658:670	the biopolymer nanoparticles	643:670	the biopolymer nanoparticles	643:670	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	5	27	theme	scavenging	803:812	arg1	activity					814:821	The DPPH radical scavenging activity	786:821	The DPPH radical scavenging activity of the curcumin	786:837	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	1	28	dep	encapsulation	201:213	arg1	the					197:199	the	197:199	the	197:199	In this study, core-shell biopolymer nanoparticles were fabricated for the encapsulation and delivery of curcumin using a pH-driven method.					
33799264	1	29	theme	curcumin	231:238	arg1	encapsulation					201:213	encapsulation	201:213	encapsulation	201:213	In this study, core-shell biopolymer nanoparticles were fabricated for the encapsulation and delivery of curcumin using a pH-driven method.					
33799264	1	29	theme	curcumin	231:238	arg1	delivery					219:226	delivery	219:226	delivery	219:226	In this study, core-shell biopolymer nanoparticles were fabricated for the encapsulation and delivery of curcumin using a pH-driven method.					
33799264	3	30	theme	diffraction	475:485	arg1	analyses					487:494	X-ray diffraction analyses	469:494	X-ray diffraction analyses	469:494	Fourier transform infrared spectroscopy and X-ray diffraction analyses indicated that curcumin was encapsulated in an amorphous state inside the nanoparticles.					
33799264	4	31	theme	ζ-potential	603:613	arg1	measurements					615:626	ζ-potential measurements	603:626	ζ-potential measurements	603:626	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	6	32	theme	electrostatic	1109:1121	arg1	repulsion					1123:1131	the steric and electrostatic repulsion	1094:1131	the steric and electrostatic repulsion between them	1094:1144	These results were attributed to the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin.					
33799264	0	33	theme	pH-driven	68:76	arg1	method					78:83	the pH-driven method	64:83	the pH-driven method	64:83	Curcumin-loaded core-shell biopolymer nanoparticles produced by the pH-driven method: Physicochemical and release properties.					
33799264	6	34	theme	thick	1040:1044	arg1	layer					1046:1050	a thick layer	1038:1050	a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them	1038:1144	These results were attributed to the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin.					
33799264	5	35	dep	increased	843:851	arg1	whereas					899:905	whereas	899:905	whereas	899:905	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	2	36	dep	properties	331:340	arg1	the					311:313	the	311:313	the	311:313	The influences of the coating composition on the physicochemical properties and curcumin release characteristics of the core-shell nanoparticles were studied.					
33799264	4	37	theme	different	701:709	arg1	storage					747:753	long term storage	737:753	long term storage	737:753	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	37	theme	different	701:709	arg1	conditions					725:734	different environmental conditions	701:734	different environmental conditions: long term storage, heating, pH changes and salt	701:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	37	theme	different	701:709	arg1	salt					780:783	salt	780:783	salt	780:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	37	theme	different	701:709	arg1	heating					756:762	heating	756:762	heating	756:762	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	38	dep	conditions	725:734	arg1	storage					747:753	long term storage	737:753	long term storage	737:753	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	38	dep	conditions	725:734	arg1	conditions					725:734	different environmental conditions	701:734	different environmental conditions: long term storage, heating, pH changes and salt	701:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	38	dep	conditions	725:734	arg1	salt					780:783	salt	780:783	salt	780:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	38	dep	conditions	725:734	arg1	heating					756:762	heating	756:762	heating	756:762	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	38	dep	conditions	725:734	arg1	changes					768:774	pH changes	765:774	pH changes	765:774	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	0	39	dep	nanoparticles	38:50	arg1	produced					52:59	produced	52:59	nanoparticles produced by the pH-driven method: Physicochemical and release properties	38:123	Curcumin-loaded core-shell biopolymer nanoparticles produced by the pH-driven method: Physicochemical and release properties.					
33799264	3	40	theme	amorphous	543:551	arg1	state					553:557	an amorphous state	540:557	an amorphous state inside the nanoparticles	540:582	Fourier transform infrared spectroscopy and X-ray diffraction analyses indicated that curcumin was encapsulated in an amorphous state inside the nanoparticles.					
33799264	4	41	theme	environmental	711:723	arg1	storage					747:753	long term storage	737:753	long term storage	737:753	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	41	theme	environmental	711:723	arg1	conditions					725:734	different environmental conditions	701:734	different environmental conditions: long term storage, heating, pH changes and salt	701:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	41	theme	environmental	711:723	arg1	salt					780:783	salt	780:783	salt	780:783	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	4	41	theme	environmental	711:723	arg1	heating					756:762	heating	756:762	heating	756:762	Particle size and ζ-potential measurements indicated that the biopolymer nanoparticles were relatively stable under different environmental conditions: long term storage, heating, pH changes and salt.					
33799264	1	42	theme	core-shell	141:150	arg1	nanoparticles					163:175	core-shell biopolymer nanoparticles	141:175	core-shell biopolymer nanoparticles	141:175	In this study, core-shell biopolymer nanoparticles were fabricated for the encapsulation and delivery of curcumin using a pH-driven method.					
33799264	1	43	theme	pH-driven	248:256	arg1	method					258:263	a pH-driven method	246:263	a pH-driven method	246:263	In this study, core-shell biopolymer nanoparticles were fabricated for the encapsulation and delivery of curcumin using a pH-driven method.					
33799264	5	44	theme	curcumin	939:946	arg1	prolonged					952:960	prolonged	952:960	prolonged	952:960	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	5	44	theme	curcumin	939:946	arg1	release					928:934	the gastrointestinal release	907:934	the gastrointestinal release of curcumin	907:946	The DPPH radical scavenging activity of the curcumin was increased after encapsulation within the nanoparticles, whereas the gastrointestinal release of curcumin was prolonged.					
33799264	1	45	theme	biopolymer	152:161	arg1	nanoparticles					163:175	core-shell biopolymer nanoparticles	141:175	core-shell biopolymer nanoparticles	141:175	In this study, core-shell biopolymer nanoparticles were fabricated for the encapsulation and delivery of curcumin using a pH-driven method.					
33799264	6	46	theme	alginate	1011:1018	arg1	ability					1000:1006	the ability	996:1006	the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin	996:1191	These results were attributed to the ability of alginate and NaCas to form a thick layer around the nanoparticles, which increased the steric and electrostatic repulsion between them, as well as inhibiting the release of curcumin.					
32053641	5	0	theme	better	1028:1033	arg1	resistance					1035:1044	better resistance	1028:1044	better resistance to light and water	1028:1063	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	6	1	theme	blue-violet	1200:1210	arg1	light					1212:1216	blue-violet light	1200:1216	blue-violet light	1200:1216	More interestingly, we found that after adding cyanobacteria, the poly(vinyl)alcohol-based films present better barrier properties to blue-violet light and red light.					
32053641	4	2	theme	cellulose	694:702	arg1	alcohol					726:732	cellulose nanofibers/poly(vinyl)alcohol	694:732	cellulose nanofibers/poly(vinyl)alcohol	694:732	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	1	3	theme	cyanobacteria	141:153	arg1	blooms					155:160	The harmful cyanobacteria blooms	129:160	The harmful cyanobacteria blooms	129:160	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	1	4	theme	freshwater	234:243	arg1	ecosystems					264:273	freshwater and coastal marine ecosystems	234:273	ecosystems	264:273	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	5	5	theme	vinyl	880:884	arg1	alcohol					886:892	cellulose nanofibers/poly(vinyl)alcohol	854:892	cellulose nanofibers/poly(vinyl)alcohol	854:892	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	7	6	theme	composites	1413:1422	arg1	roughness					1369:1377	surface roughness	1361:1377	surface roughness	1361:1377	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	7	6	theme	composites	1413:1422	arg1	angle					1391:1395	contact angle	1383:1395	contact angle	1383:1395	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	5	7	theme	cellulose	854:862	arg1	alcohol					886:892	cellulose nanofibers/poly(vinyl)alcohol	854:892	cellulose nanofibers/poly(vinyl)alcohol	854:892	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	5	8	theme	nanofibers/poly	864:878	arg1	alcohol					886:892	cellulose nanofibers/poly(vinyl)alcohol	854:892	cellulose nanofibers/poly(vinyl)alcohol	854:892	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	7	9	theme	vinyl	1283:1287	arg1	alcohol					1289:1295	poly(vinyl)alcohol	1278:1295	poly(vinyl)alcohol	1278:1295	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	5	10	theme	vinyl	995:999	arg1	alcohol					1001:1007	the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol	951:1007	the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol	951:1007	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	4	11	theme	barrier	756:762	arg1	properties					764:773	the barrier properties	752:773	the barrier properties of poly(vinyl)alcohol to light and water	752:814	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	1	12	theme	coastal	249:255	arg1	ecosystems					264:273	freshwater and coastal marine ecosystems	234:273	ecosystems	264:273	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	0	13	theme	blue-violet	88:98	arg1	light					100:104	blue-violet light	88:104	blue-violet light / red light and water	88:126	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	3	14	theme	carboxymethyl	478:490	arg1	cellulose					492:500	carboxymethyl cellulose	478:500	carboxymethyl cellulose	478:500	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	1	15	theme	marine	257:262	arg1	ecosystems					264:273	freshwater and coastal marine ecosystems	234:273	ecosystems	264:273	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	0	16	theme	red	108:110	arg1	light					112:116	red light	108:116	red light	108:116	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	6	17	theme	red	1222:1224	arg1	light					1226:1230	red light	1222:1230	red light	1222:1230	More interestingly, we found that after adding cyanobacteria, the poly(vinyl)alcohol-based films present better barrier properties to blue-violet light and red light.					
32053641	0	18	dep	light	100:104	arg1	water					122:126	water	122:126	water	122:126	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	0	18	dep	light	100:104	arg1	light					112:116	red light	108:116	red light	108:116	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	5	19	theme	vinyl	836:840	arg1	alcohol					842:848	poly(vinyl)alcohol	831:848	poly(vinyl)alcohol	831:848	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	2	20	theme	alcohol-based	369:381	arg1	films					383:387	biological poly(vinyl)alcohol-based films	347:387	biological poly(vinyl)alcohol-based films	347:387	This study tried to utilize cyanobacteria as a raw material to produce biological poly(vinyl)alcohol-based films.					
32053641	7	21	theme	nanofibers/poly	1310:1324	arg1	matrix					1340:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	4	22	theme	vinyl	676:680	arg1	alcohol					682:688	poly(vinyl)alcohol	671:688	poly(vinyl)alcohol	671:688	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	1	23	theme	ecosystems	264:273	arg1	eutrophication					216:229	global eutrophication	209:229	global eutrophication of freshwater and coastal marine ecosystems	209:273	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	4	24	theme	vinyl	720:724	arg1	alcohol					726:732	cellulose nanofibers/poly(vinyl)alcohol	694:732	cellulose nanofibers/poly(vinyl)alcohol	694:732	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	5	25	theme	poly	831:834	arg1	alcohol					842:848	poly(vinyl)alcohol	831:848	poly(vinyl)alcohol	831:848	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	7	26	theme	surface	1361:1367	arg1	roughness					1369:1377	surface roughness	1361:1377	surface roughness	1361:1377	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	7	27	theme	contact	1383:1389	arg1	angle					1391:1395	contact angle	1383:1395	contact angle	1383:1395	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	5	28	theme	alcohol	886:892	arg1	films					894:898	poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films	831:898	poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films	831:898	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	2	29	dep	material	327:334	arg1	produce					339:345	produce	339:345	to produce biological poly(vinyl)alcohol-based films	336:387	This study tried to utilize cyanobacteria as a raw material to produce biological poly(vinyl)alcohol-based films.					
32053641	5	30	theme	cyanobacteria/poly	914:931	arg1	alcohol					939:945	cyanobacteria/poly(vinyl)alcohol	914:945	cyanobacteria/poly(vinyl)alcohol	914:945	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	0	31	theme	cyanobacteria-enhanced	15:36	arg1	films					63:67	cyanobacteria-enhanced poly(vinyl)alcohol-based films	15:67	cyanobacteria-enhanced poly(vinyl)alcohol-based films	15:67	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	4	32	dep	alcohol	682:688	arg1	matrix					734:739	matrix	734:739	matrix	734:739	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	5	33	theme	obtained	905:912	arg1	composites					1009:1018	the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites	901:1018	the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites	901:1018	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	4	34	theme	modified	630:637	arg1	cyanobacteria					639:651	And then the modified cyanobacteria	617:651	cyanobacteria	639:651	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	4	35	theme	nanofibers/poly	704:718	arg1	alcohol					726:732	cellulose nanofibers/poly(vinyl)alcohol	694:732	cellulose nanofibers/poly(vinyl)alcohol	694:732	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	6	36	theme	barrier	1178:1184	arg1	properties					1186:1195	better barrier properties	1171:1195	better barrier properties	1171:1195	More interestingly, we found that after adding cyanobacteria, the poly(vinyl)alcohol-based films present better barrier properties to blue-violet light and red light.					
32053641	5	37	theme	nanofibers/poly	979:993	arg1	alcohol					1001:1007	the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol	951:1007	the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol	951:1007	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	4	38	theme	poly	671:674	arg1	alcohol					682:688	poly(vinyl)alcohol	671:688	poly(vinyl)alcohol	671:688	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	4	39	theme	alcohol	789:795	arg1	properties					764:773	the barrier properties	752:773	the barrier properties of poly(vinyl)alcohol to light and water	752:814	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	7	40	theme	vinyl	1326:1330	arg1	matrix					1340:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	3	41	theme	ethylene	435:442	arg1	poly					430:433	poly	430:433	poly(ethylene glycol)	430:450	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	41	theme	ethylene	435:442	arg1	glycol					444:449	ethylene glycol	435:449	ethylene glycol	435:449	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	0	42	theme	alcohol-based	49:61	arg1	films					63:67	cyanobacteria-enhanced poly(vinyl)alcohol-based films	15:67	cyanobacteria-enhanced poly(vinyl)alcohol-based films	15:67	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	5	43	theme	alcohol	842:848	arg1	films					894:898	poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films	831:898	poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films	831:898	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	3	44	theme	coupling	587:594	arg1	agent					596:600	coupling agent	587:600	coupling agent	587:600	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	7	45	dep	roughness	1369:1377	arg1	the					1357:1359	the	1357:1359	the	1357:1359	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	5	46	theme	alcohol	1001:1007	arg1	composites					1009:1018	the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites	901:1018	the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites	901:1018	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	6	47	theme	better	1171:1176	arg1	properties					1186:1195	better barrier properties	1171:1195	better barrier properties	1171:1195	More interestingly, we found that after adding cyanobacteria, the poly(vinyl)alcohol-based films present better barrier properties to blue-violet light and red light.					
32053641	2	48	theme	raw	323:325	arg1	material					327:334	a raw material	321:334	a raw material to produce biological poly(vinyl)alcohol-based films	321:387	This study tried to utilize cyanobacteria as a raw material to produce biological poly(vinyl)alcohol-based films.					
32053641	2	48	theme	raw	323:325	arg1	cyanobacteria					304:316	cyanobacteria	304:316	cyanobacteria	304:316	This study tried to utilize cyanobacteria as a raw material to produce biological poly(vinyl)alcohol-based films.					
32053641	5	49	theme	cyanobacteria/cellulose	955:977	arg1	alcohol					1001:1007	the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol	951:1007	the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol	951:1007	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	7	50	theme	cellulose	1300:1308	arg1	matrix					1340:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	5	51	theme	alcohol	939:945	arg1	composites					1009:1018	the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites	901:1018	the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites	901:1018	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	0	52	with	Preparation	0:10	arg1	resistance					74:83	resistance	74:83	resistance to blue-violet light / red light and water	74:126	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	4	53	theme	poly	778:781	arg1	alcohol					789:795	poly(vinyl)alcohol	778:795	poly(vinyl)alcohol to light and water	778:814	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	1	54	theme	harmful	133:139	arg1	blooms					155:160	The harmful cyanobacteria blooms	129:160	The harmful cyanobacteria blooms	129:160	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	7	55	theme	poly	1278:1281	arg1	alcohol					1289:1295	poly(vinyl)alcohol	1278:1295	poly(vinyl)alcohol	1278:1295	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32053641	1	56	theme	global	209:214	arg1	eutrophication					216:229	global eutrophication	209:229	global eutrophication of freshwater and coastal marine ecosystems	209:273	The harmful cyanobacteria blooms which usually form in spring and summer, cause global eutrophication of freshwater and coastal marine ecosystems.					
32053641	5	57	theme	vinyl	933:937	arg1	alcohol					939:945	cyanobacteria/poly(vinyl)alcohol	914:945	cyanobacteria/poly(vinyl)alcohol	914:945	Compared with poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol films, the obtained cyanobacteria/poly(vinyl)alcohol and the cyanobacteria/cellulose nanofibers/poly(vinyl)alcohol composites exhibit better resistance to light and water.					
32053641	6	58	theme	alcohol-based	1143:1155	arg1	films					1157:1161	the poly(vinyl)alcohol-based films	1128:1161	the poly(vinyl)alcohol-based films	1128:1161	More interestingly, we found that after adding cyanobacteria, the poly(vinyl)alcohol-based films present better barrier properties to blue-violet light and red light.					
32053641	3	59	mod	modified	416:423	arg3	hydrochloride					463:475	guanidine hydrochloride	453:475	guanidine hydrochloride	453:475	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	59	mod	modified	416:423	arg3	poly					430:433	poly	430:433	poly(ethylene glycol)	430:450	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	59	mod	modified	416:423	arg1	Cyanobacteria					390:402	Cyanobacteria	390:402	Cyanobacteria	390:402	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	59	mod	modified	416:423	arg1	plasticizer					543:553	plasticizer	543:553	plasticizer	543:553	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	59	mod	modified	416:423	arg3	3-glycidoxypropyltrimethoxysilane					506:538	3-glycidoxypropyltrimethoxysilane	506:538	3-glycidoxypropyltrimethoxysilane	506:538	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	59	mod	modified	416:423	arg3	glycol					444:449	ethylene glycol	435:449	ethylene glycol	435:449	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	59	mod	modified	416:423	arg3	cellulose					492:500	carboxymethyl cellulose	478:500	carboxymethyl cellulose	478:500	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	3	60	theme	guanidine	453:461	arg1	hydrochloride					463:475	guanidine hydrochloride	453:475	guanidine hydrochloride	453:475	Cyanobacteria was firstly modified with poly(ethylene glycol), guanidine hydrochloride, carboxymethyl cellulose and 3-glycidoxypropyltrimethoxysilane as plasticizer, modifier, toughening agent and coupling agent, respectively.					
32053641	0	61	theme	films	63:67	arg1	Preparation					0:10	Preparation	0:10	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.	0:127	Preparation of cyanobacteria-enhanced poly(vinyl)alcohol-based films with resistance to blue-violet light / red light and water.					
32053641	4	62	theme	vinyl	783:787	arg1	alcohol					789:795	poly(vinyl)alcohol	778:795	poly(vinyl)alcohol to light and water	778:814	And then the modified cyanobacteria was introduced to poly(vinyl)alcohol and cellulose nanofibers/poly(vinyl)alcohol matrix to improve the barrier properties of poly(vinyl)alcohol to light and water.					
32053641	2	63	theme	biological	347:356	arg1	films					383:387	biological poly(vinyl)alcohol-based films	347:387	biological poly(vinyl)alcohol-based films	347:387	This study tried to utilize cyanobacteria as a raw material to produce biological poly(vinyl)alcohol-based films.					
32053641	7	64	theme	alcohol	1332:1338	arg1	matrix					1340:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	cellulose nanofibers/poly(vinyl)alcohol matrix	1300:1345	In adddition, introducing cyanobacteria into poly(vinyl)alcohol or cellulose nanofibers/poly(vinyl)alcohol matrix increases the surface roughness and contact angle to water of the composites.					
32268183	5	0	theme	Ca-alg/	686:692	arg1	Si-HAP					698:703	Si-HAP	698:703	Si-HAP	698:703	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	5	0	theme	Ca-alg/	686:692	arg1	La					694:695	Ca-alg/ La	686:695	Ca-alg/ La	686:695	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	6	1	dep	spectroscopy	904:915	arg1	techniques					949:958	techniques	949:958	techniques	949:958	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	2	2	theme	titanium	377:384	arg1	implant					391:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	9	3	theme	Ti	1385:1386	arg1	ability					1353:1359	The osteogenic ability	1338:1359	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model	1338:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	8	4	theme	phosphate	1139:1147	arg1	activity					1153:1160	alkaline phosphate ALP activity	1130:1160	alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite)	1130:1220	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	6	5	theme	Infra-Red	894:902	arg1	spectroscopy					904:915	Infra-Red spectroscopy	894:915	Infra-Red spectroscopy (FT-IR)	894:923	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	5	theme	Infra-Red	894:902	arg1	FT-IR					918:922	FT-IR	918:922	FT-IR	918:922	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	2	6	theme	hydroxyapatite	310:323	arg1	implant					391:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	7	7	from	coating	971:977	arg1	surface					1031:1037	Ti surface	1028:1037	Ti surface	1028:1037	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	6	8	theme	PVP	794:796	arg1	polymer					798:804	PVP polymer	794:804	PVP polymer	794:804	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	9	9	theme	in-vivo	1402:1408	arg1	model					1414:1418	in-vivo rat model	1402:1418	in-vivo rat model	1402:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	3	10	with	process	453:459	arg1	assistance					466:475	assistance	466:475	assistance of calcium alginate template	466:504	The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template.					
32268183	5	11	theme	zeta	653:656	arg1	value					668:672	The negative zeta potential value -9.97 mV	640:681	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP	640:703	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	6	12	theme	minerals	781:788	arg1	Incorporation					764:776	Incorporation	764:776	Incorporation of minerals and PVP polymer	764:804	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	7	13	theme	Electrophoretic	1055:1069	arg1	method					1088:1093	Electrophoretic deposition (EPD) method	1055:1093	Electrophoretic deposition (EPD) method	1055:1093	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	8	14	used	utilized	1227:1234	arg2	activity					1153:1160	alkaline phosphate ALP activity	1130:1160	alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite)	1130:1220	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	14	used	utilized	1227:1234	arg2	method					1119:1124	The in-vitro MTT assay method	1096:1124	The in-vitro MTT assay method	1096:1124	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	9	15	theme	Ti	1457:1458	arg1	plate					1460:1464	the fabricated Ti plate	1442:1464	the fabricated Ti plate with bio-composite coatings	1442:1492	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	5	16	from	incorporation	728:740	arg1	matrix					756:761	the matrix	752:761	the matrix	752:761	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	6	17	theme	Electron	929:936	arg1	Microscopy					938:947	Electron Microscopy	929:947	Electron Microscopy	929:947	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	8	18	theme	cell	1253:1256	arg1	viability					1258:1266	cell viability	1253:1266	cell viability	1253:1266	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	5	19	theme	La	694:695	arg1	value					668:672	The negative zeta potential value -9.97 mV	640:681	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP	640:703	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	2	20	theme	lanthanum	259:267	arg1	ability					248:254	the osteogenic ability	233:254	the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	233:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	1	21	theme	great	153:157	arg1	significance					159:170	great significance	153:170	great significance for improving the bone regeneration	153:206	Osteogenic differentiation is great significance for improving the bone regeneration.					
32268183	7	22	with	composite	986:994	arg1	thickness					1005:1013	the thickness	1001:1013	the thickness of 448 nm	1001:1023	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	8	23	dep	activity	1153:1160	arg1	%					1165:1165	94%	1163:1165	94%	1163:1165	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	23	dep	activity	1153:1160	arg1	a.u					1176:1178	0.94 a.u	1171:1178	0.94 a.u	1171:1178	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	24	theme	Bone	1297:1300	arg1	Cells					1322:1326	human Bone Marrow-Derived Stem Cells	1291:1326	human Bone Marrow-Derived Stem Cells (hBMSCs)	1291:1335	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	24	theme	Bone	1297:1300	arg1	hBMSCs					1329:1334	hBMSCs	1329:1334	hBMSCs	1329:1334	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	0	25	theme	tibia	98:102	arg1	regeneration					109:120	tibia bone regeneration	98:120	tibia bone regeneration	98:120	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	8	26	theme	Stem	1317:1320	arg1	Cells					1322:1326	human Bone Marrow-Derived Stem Cells	1291:1326	human Bone Marrow-Derived Stem Cells (hBMSCs)	1291:1335	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	26	theme	Stem	1317:1320	arg1	hBMSCs					1329:1334	hBMSCs	1329:1334	hBMSCs	1329:1334	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	9	27	theme	promising	1505:1513	arg1	biomaterial					1515:1525	promising biomaterial	1505:1525	promising biomaterial for orthopedics	1505:1541	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	9	28	with	plate	1460:1464	arg1	coatings					1485:1492	bio-composite coatings	1471:1492	bio-composite coatings	1471:1492	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	3	29	theme	hydrothermal	440:451	arg1	process					453:459	hydrothermal process	440:459	hydrothermal process with assistance of calcium alginate template	440:504	The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template.					
32268183	0	30	theme	Calcium	0:6	arg1	template-mineral					17:32	Calcium alginate template-mineral	0:32	Calcium alginate template-mineral	0:32	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	3	31	theme	alginate	488:495	arg1	template					497:504	calcium alginate template	480:504	calcium alginate template	480:504	The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template.					
32268183	9	32	from	hBMSCs	1391:1396	arg1	ability					1353:1359	The osteogenic ability	1338:1359	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model	1338:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	0	33	theme	hydroxyapatite	46:59	arg1	implant					86:92	hydroxyapatite hydrogel coated titanium implant	46:92	hydroxyapatite hydrogel coated titanium implant	46:92	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	9	34	from	Ti	1385:1386	arg1	model					1414:1418	in-vivo rat model	1402:1418	in-vivo rat model	1402:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	9	34	from	Ti	1385:1386	arg1	hBMSCs					1391:1396	hBMSCs	1391:1396	hBMSCs	1391:1396	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	2	35	theme	treated	369:375	arg1	Ti					387:388	Ti	387:388	Ti	387:388	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	2	35	theme	treated	369:375	arg1	titanium					377:384	polymeric composite coated surface treated titanium	334:384	polymeric composite coated surface treated titanium (Ti)	334:389	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	0	36	theme	coated	70:75	arg1	implant					86:92	hydroxyapatite hydrogel coated titanium implant	46:92	hydroxyapatite hydrogel coated titanium implant	46:92	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	6	37	theme	Dispersive	843:852	arg1	Fourier					876:882	Fourier	876:882	Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques	876:958	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	37	theme	Dispersive	843:852	arg1	analysis					860:867	Energy Dispersive X-ray analysis	836:867	Energy Dispersive X-ray analysis (EDX)	836:873	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	37	theme	Dispersive	843:852	arg1	EDX					870:872	EDX	870:872	EDX	870:872	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	4	38	theme	vinyl	557:561	arg1	poly					551:554	the polymer poly	539:554	the polymer poly (vinyl pyrrolidone) (PVP)	539:580	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	4	38	theme	vinyl	557:561	arg1	pyrrolidone					563:573	vinyl pyrrolidone	557:573	vinyl pyrrolidone	557:573	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	2	39	theme	coated	354:359	arg1	Ti					387:388	Ti	387:388	Ti	387:388	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	2	39	theme	coated	354:359	arg1	titanium					377:384	polymeric composite coated surface treated titanium	334:384	polymeric composite coated surface treated titanium (Ti)	334:389	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	9	40	theme	osteogenic	1342:1351	arg1	ability					1353:1359	The osteogenic ability	1338:1359	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model	1338:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	9	41	from	ability	1353:1359	arg1	model					1414:1418	in-vivo rat model	1402:1418	in-vivo rat model	1402:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	9	41	from	ability	1353:1359	arg1	hBMSCs					1391:1396	hBMSCs	1391:1396	hBMSCs	1391:1396	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	8	42	dep	viability	1258:1266	arg1	the					1249:1251	the	1249:1251	the	1249:1251	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	43	theme	MTT	1109:1111	arg1	assay					1113:1117	The in-vitro MTT assay	1096:1117	The in-vitro MTT assay method	1096:1124	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	7	44	from	surface	1031:1037	arg1	coating					971:977	A compact coating	961:977	A compact coating of the composite with the thickness of 448 nm on Ti surface	961:1037	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	5	45	theme	potential	658:666	arg1	value					668:672	The negative zeta potential value -9.97 mV	640:681	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP	640:703	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	9	46	theme	coated	1378:1383	arg1	Ti					1385:1386	bio-composite coated Ti	1364:1386	bio-composite coated Ti in hBMSCs and in-vivo rat model	1364:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	5	47	theme	negative	644:651	arg1	value					668:672	The negative zeta potential value -9.97 mV	640:681	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP	640:703	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	8	48	theme	alkaline	1130:1137	arg1	activity					1153:1160	alkaline phosphate ALP activity	1130:1160	alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite)	1130:1220	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	7	49	theme	EPD	1083:1085	arg1	method					1088:1093	Electrophoretic deposition (EPD) method	1055:1093	Electrophoretic deposition (EPD) method	1055:1093	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	6	50	theme	polymer	798:804	arg1	Incorporation					764:776	Incorporation	764:776	Incorporation of minerals and PVP polymer	764:804	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	2	51	theme	substituted	298:308	arg1	implant					391:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	8	52	theme	ALP	1149:1151	arg1	activity					1153:1160	alkaline phosphate ALP activity	1130:1160	alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite)	1130:1220	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	7	53	theme	deposition	1071:1080	arg1	method					1088:1093	Electrophoretic deposition (EPD) method	1055:1093	Electrophoretic deposition (EPD) method	1055:1093	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	2	54	theme	polymeric	334:342	arg1	Ti					387:388	Ti	387:388	Ti	387:388	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	2	54	theme	polymeric	334:342	arg1	titanium					377:384	polymeric composite coated surface treated titanium	334:384	polymeric composite coated surface treated titanium (Ti)	334:389	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	9	55	theme	rat	1410:1412	arg1	model					1414:1418	in-vivo rat model	1402:1418	in-vivo rat model	1402:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	7	56	from	composite	986:994	arg1	surface					1031:1037	Ti surface	1028:1037	Ti surface	1028:1037	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	2	57	dep	lanthanum	259:267	arg1	implant					391:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	298:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	7	58	theme	Ti	1028:1029	arg1	surface					1031:1037	Ti surface	1028:1037	Ti surface	1028:1037	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	9	59	theme	fabricated	1446:1455	arg1	plate					1460:1464	the fabricated Ti plate	1442:1464	the fabricated Ti plate with bio-composite coatings	1442:1492	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	7	60	theme	448 nm	1018:1023	arg1	thickness					1005:1013	the thickness	1001:1013	the thickness of 448 nm	1001:1023	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	8	61	from	viability	1258:1266	arg1	Cells					1322:1326	human Bone Marrow-Derived Stem Cells	1291:1326	human Bone Marrow-Derived Stem Cells (hBMSCs)	1291:1335	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	61	from	viability	1258:1266	arg1	hBMSCs					1329:1334	hBMSCs	1329:1334	hBMSCs	1329:1334	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	2	62	theme	silicate	280:287	arg1	ability					248:254	the osteogenic ability	233:254	the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	233:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	7	63	theme	composite	986:994	arg1	coating					971:977	A compact coating	961:977	A compact coating of the composite with the thickness of 448 nm on Ti surface	961:1037	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	9	64	theme	bio-composite	1471:1483	arg1	coatings					1485:1492	bio-composite coatings	1471:1492	bio-composite coatings	1471:1492	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	0	65	theme	bone	104:107	arg1	regeneration					109:120	tibia bone regeneration	98:120	tibia bone regeneration	98:120	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	1	66	theme	bone	190:193	arg1	regeneration					195:206	the bone regeneration	186:206	the bone regeneration	186:206	Osteogenic differentiation is great significance for improving the bone regeneration.					
32268183	4	67	theme	polymer	543:549	arg1	poly					551:554	the polymer poly	539:554	the polymer poly (vinyl pyrrolidone) (PVP)	539:580	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	4	67	theme	polymer	543:549	arg1	pyrrolidone					563:573	vinyl pyrrolidone	557:573	vinyl pyrrolidone	557:573	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	4	67	theme	polymer	543:549	arg1	PVP					577:579	PVP	577:579	PVP	577:579	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	8	68	theme	human	1291:1295	arg1	Cells					1322:1326	human Bone Marrow-Derived Stem Cells	1291:1326	human Bone Marrow-Derived Stem Cells (hBMSCs)	1291:1335	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	68	theme	human	1291:1295	arg1	hBMSCs					1329:1334	hBMSCs	1329:1334	hBMSCs	1329:1334	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	4	69	theme	ultra-sonication	615:630	arg1	method					632:637	ultra-sonication method	615:637	ultra-sonication method	615:637	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	7	70	theme	compact	963:969	arg1	coating					971:977	A compact coating	961:977	A compact coating of the composite with the thickness of 448 nm on Ti surface	961:1037	A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method.					
32268183	8	71	theme	Marrow-Derived	1302:1315	arg1	Cells					1322:1326	human Bone Marrow-Derived Stem Cells	1291:1326	human Bone Marrow-Derived Stem Cells (hBMSCs)	1291:1335	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	71	theme	Marrow-Derived	1302:1315	arg1	hBMSCs					1329:1334	hBMSCs	1329:1334	hBMSCs	1329:1334	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	5	72	theme	PVP	745:747	arg1	incorporation					728:740	the incorporation	724:740	the incorporation of PVP in the matrix	724:761	The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix.					
32268183	0	73	theme	alginate	8:15	arg1	template-mineral					17:32	Calcium alginate template-mineral	0:32	Calcium alginate template-mineral	0:32	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	3	74	theme	bio-ceramic	404:414	arg1	MHAP					416:419	The bio-ceramic MHAP	400:419	The bio-ceramic MHAP	400:419	The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template.					
32268183	8	75	theme	optimized	1201:1209	arg1	composite					1211:1219	the optimized composite	1197:1219	the optimized composite	1197:1219	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	76	from	differentiation	1272:1286	arg1	Cells					1322:1326	human Bone Marrow-Derived Stem Cells	1291:1326	human Bone Marrow-Derived Stem Cells (hBMSCs)	1291:1335	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	8	76	from	differentiation	1272:1286	arg1	hBMSCs					1329:1334	hBMSCs	1329:1334	hBMSCs	1329:1334	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	1	77	theme	Osteogenic	123:132	arg1	differentiation					134:148	Osteogenic differentiation	123:148	Osteogenic differentiation	123:148	Osteogenic differentiation is great significance for improving the bone regeneration.					
32268183	3	78	theme	template	497:504	arg1	assistance					466:475	assistance	466:475	assistance of calcium alginate template	466:504	The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template.					
32268183	0	79	theme	hydrogel	61:68	arg1	implant					86:92	hydroxyapatite hydrogel coated titanium implant	46:92	hydroxyapatite hydrogel coated titanium implant	46:92	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	2	80	theme	Present	209:215	arg1	study					217:221	Present study	209:221	Present study	209:221	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	3	81	theme	calcium	480:486	arg1	template					497:504	calcium alginate template	480:504	calcium alginate template	480:504	The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template.					
32268183	0	82	theme	titanium	77:84	arg1	implant					86:92	hydroxyapatite hydrogel coated titanium implant	46:92	hydroxyapatite hydrogel coated titanium implant	46:92	Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration.					
32268183	6	83	theme	X-ray	854:858	arg1	Fourier					876:882	Fourier	876:882	Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques	876:958	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	83	theme	X-ray	854:858	arg1	analysis					860:867	Energy Dispersive X-ray analysis	836:867	Energy Dispersive X-ray analysis (EDX)	836:873	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	83	theme	X-ray	854:858	arg1	EDX					870:872	EDX	870:872	EDX	870:872	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	2	84	theme	surface	361:367	arg1	Ti					387:388	Ti	387:388	Ti	387:388	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	2	84	theme	surface	361:367	arg1	titanium					377:384	polymeric composite coated surface treated titanium	334:384	polymeric composite coated surface treated titanium (Ti)	334:389	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	4	85	dep	hydrophilicity	523:536	arg1	enhance					511:517	enhance	511:517	enhance	511:517	For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method.					
32268183	8	86	theme	in-vitro	1100:1107	arg1	assay					1113:1117	The in-vitro MTT assay	1096:1117	The in-vitro MTT assay method	1096:1124	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	6	87	theme	Energy	836:841	arg1	Fourier					876:882	Fourier	876:882	Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques	876:958	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	87	theme	Energy	836:841	arg1	analysis					860:867	Energy Dispersive X-ray analysis	836:867	Energy Dispersive X-ray analysis (EDX)	836:873	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	6	87	theme	Energy	836:841	arg1	EDX					870:872	EDX	870:872	EDX	870:872	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	2	88	theme	osteogenic	237:246	arg1	ability					248:254	the osteogenic ability	233:254	the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant	233:397	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	2	89	theme	composite	344:352	arg1	Ti					387:388	Ti	387:388	Ti	387:388	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	2	89	theme	composite	344:352	arg1	titanium					377:384	polymeric composite coated surface treated titanium	334:384	polymeric composite coated surface treated titanium (Ti)	334:389	Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant.					
32268183	8	90	theme	assay	1113:1117	arg1	method					1119:1124	The in-vitro MTT assay method	1096:1124	The in-vitro MTT assay method	1096:1124	The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs).					
32268183	9	91	from	model	1414:1418	arg1	ability					1353:1359	The osteogenic ability	1338:1359	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model	1338:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
32268183	6	92	dep	Fourier	876:882	arg1	Transform					884:892	Transform	884:892	Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques	884:958	Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques.					
32268183	9	93	theme	bio-composite	1364:1376	arg1	Ti					1385:1386	bio-composite coated Ti	1364:1386	bio-composite coated Ti in hBMSCs and in-vivo rat model	1364:1418	The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.					
34280803	0	0	theme	acidic	91:96	arg1	exopolysaccharide					98:114	linckia acidic exopolysaccharide	83:114	linckia acidic exopolysaccharide	83:114	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
34280803	6	1	located	found	779:783	arg2	stability					765:773	Low stability	761:773	Low stability	761:773	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	6	1	located	found	779:783	arg1	conditions					799:808	all tested conditions	788:808	all tested conditions	788:808	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	7	2	theme	forms	1087:1091	arg1	quantification					1060:1073	simultaneous quantification	1047:1073	simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone	1047:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	0	3	theme	linckia	83:89	arg1	exopolysaccharide					98:114	linckia acidic exopolysaccharide	83:114	linckia acidic exopolysaccharide	83:114	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
34280803	7	4	dep	one	1113:1115	arg1	one					1103:1105	the free one	1094:1105	the free one	1094:1105	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	7	4	dep	one	1113:1115	arg1	linked					1117:1122	linked	1117:1122	linked in oligosaccharides, as well as GlcA degradation product γ-lactone	1117:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	8	5	from	content	1255:1261	arg1	EPS					1266:1268	EPS	1266:1268	EPS	1266:1268	NMR as detection method improves information about uronic acid content in EPS.					
34280803	3	6	theme	hydrolysis	390:399	arg1	conditions					365:374	optimal conditions	357:374	optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	357:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	6	7	theme	%	904:904	arg1	%					911:911	25% to 40%	902:911	25% to 40%	902:911	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	6	8	dep	%	911:911	arg1	to					906:907	to	906:907	to	906:907	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	7	9	dep	analysis	1015:1022	arg1	HPAEC					1031:1035	HPAEC	1031:1035	HPAEC	1031:1035	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	7	9	dep	analysis	1015:1022	arg1	HPLC					1025:1028	HPLC	1025:1028	HPLC	1025:1028	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	7	10	theme	GlcA	1082:1085	arg1	forms					1087:1091	all GlcA forms	1078:1091	all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone	1078:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	8	11	theme	acid	1250:1253	arg1	content					1255:1261	uronic acid content	1243:1261	uronic acid content in EPS	1243:1268	NMR as detection method improves information about uronic acid content in EPS.					
34280803	3	12	theme	total	379:383	arg1	hydrolysis					390:399	total acid hydrolysis	379:399	total acid hydrolysis using trifluoroacetic acid (TFA)	379:432	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	8	13	theme	uronic	1243:1248	arg1	content					1255:1261	uronic acid content	1243:1261	uronic acid content in EPS	1243:1268	NMR as detection method improves information about uronic acid content in EPS.					
34280803	1	14	from	important	171:179	arg1	elucidation					206:216	the primary structure elucidation	184:216	the primary structure elucidation of polysaccharides	184:235	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	1	15	theme	primary	188:194	arg1	elucidation					206:216	the primary structure elucidation	184:216	the primary structure elucidation of polysaccharides	184:235	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	3	16	theme	acid	385:388	arg1	hydrolysis					390:399	total acid hydrolysis	379:399	total acid hydrolysis using trifluoroacetic acid (TFA)	379:432	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	3	17	theme	acidic	437:442	arg1	EPS					493:495	EPS	493:495	EPS	493:495	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	3	17	theme	acidic	437:442	arg1	exopolysaccharide					474:490	acidic lactylated Nostoc cf. linckia exopolysaccharide	437:490	acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	437:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	6	18	theme	tested	792:797	arg1	conditions					799:808	all tested conditions	788:808	all tested conditions	788:808	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	1	19	theme	structure	196:204	arg1	elucidation					206:216	the primary structure elucidation	184:216	the primary structure elucidation of polysaccharides	184:235	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	0	20	theme	acid	11:14	arg1	hydrolysis					16:25	Optimizing acid hydrolysis	0:25	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc	0:77	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
34280803	3	21	theme	optimal	357:363	arg1	conditions					365:374	optimal conditions	357:374	optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	357:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	7	22	theme	GlcA	1156:1159	arg1	γ-lactone					1181:1189	GlcA degradation product γ-lactone	1156:1189	GlcA degradation product γ-lactone	1156:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	7	23	attach	linked	1117:1122	arg3	oligosaccharides					1127:1142	oligosaccharides	1127:1142	oligosaccharides	1127:1142	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	7	23	attach	linked	1117:1122	arg3	γ-lactone					1181:1189	GlcA degradation product γ-lactone	1156:1189	GlcA degradation product γ-lactone	1156:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	3	24	theme	Nostoc	455:460	arg1	EPS					493:495	EPS	493:495	EPS	493:495	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	3	24	theme	Nostoc	455:460	arg1	exopolysaccharide					474:490	acidic lactylated Nostoc cf. linckia exopolysaccharide	437:490	acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	437:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	0	25	theme	Optimizing	0:9	arg1	hydrolysis					16:25	Optimizing acid hydrolysis	0:25	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc	0:77	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
34280803	4	26	theme	acid	586:589	arg1	concentration					591:603	modified acid concentration	577:603	modified acid concentration	577:603	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	7	27	theme	simultaneous	1047:1058	arg1	quantification					1060:1073	simultaneous quantification	1047:1073	simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone	1047:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	4	28	with	experiments	560:570	arg1	time					622:625	time	622:625	time	622:625	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	28	with	experiments	560:570	arg1	temperature					606:616	temperature	606:616	temperature	606:616	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	28	with	experiments	560:570	arg1	concentration					591:603	modified acid concentration	577:603	modified acid concentration	577:603	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	5	29	theme	components	731:740	arg1	stability					699:707	The stability	695:707	The stability of EPS monosaccharide components	695:740	The stability of EPS monosaccharide components was also explored.					
34280803	3	30	theme	trifluoroacetic	407:421	arg1	TFA					429:431	TFA	429:431	TFA	429:431	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	3	30	theme	trifluoroacetic	407:421	arg1	acid					423:426	trifluoroacetic acid	407:426	trifluoroacetic acid (TFA)	407:432	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	4	31	theme	modified	577:584	arg1	concentration					591:603	modified acid concentration	577:603	modified acid concentration	577:603	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	1	32	theme	polysaccharides	221:235	arg1	elucidation					206:216	the primary structure elucidation	184:216	the primary structure elucidation of polysaccharides	184:235	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	6	33	theme	hydrolysis	843:852	arg1	hour					835:838	the first hour	825:838	the first hour of hydrolysis	825:852	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	1	34	theme	exact	121:125	arg1	important					171:179	important	171:179	important	171:179	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	1	34	theme	exact	121:125	arg1	estimation					127:136	The exact estimation	117:136	The exact estimation of monosaccharide composition	117:166	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	4	35	theme	2 M	653:655	arg1	3 h					670:672	3 h	670:672	3 h	670:672	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	35	theme	2 M	653:655	arg1	110 °C					662:667	110 °C	662:667	110 °C	662:667	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	35	theme	2 M	653:655	arg1	TFA					657:659	2 M TFA	653:659	2 M TFA	653:659	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	35	theme	2 M	653:655	arg1	optimal					686:692	optimal	686:692	optimal	686:692	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	0	36	theme	compositional	46:58	arg1	analysis					60:67	monosaccharide compositional analysis	31:67	monosaccharide compositional analysis of Nostoc	31:77	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
34280803	5	37	theme	EPS	712:714	arg1	components					731:740	EPS monosaccharide components	712:740	EPS monosaccharide components	712:740	The stability of EPS monosaccharide components was also explored.					
34280803	6	38	theme	neutral	859:865	arg1	monosaccharides					867:881	all neutral monosaccharides	855:881	all neutral monosaccharides	855:881	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	0	39	theme	monosaccharide	31:44	arg1	analysis					60:67	monosaccharide compositional analysis	31:67	monosaccharide compositional analysis of Nostoc	31:77	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
34280803	3	40	theme	lactylated	444:453	arg1	EPS					493:495	EPS	493:495	EPS	493:495	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	3	40	theme	lactylated	444:453	arg1	exopolysaccharide					474:490	acidic lactylated Nostoc cf. linckia exopolysaccharide	437:490	acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	437:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	7	41	dep	forms	1087:1091	arg1	one					1103:1105	the free one	1094:1105	the free one	1094:1105	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	8	42	theme	detection	1199:1207	arg1	method					1209:1214	detection method	1199:1214	detection method	1199:1214	NMR as detection method improves information about uronic acid content in EPS.					
34280803	4	43	theme	series	550:555	arg1	Results					537:543	Results	537:543	Results	537:543	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	7	44	theme	product	1173:1179	arg1	γ-lactone					1181:1189	GlcA degradation product γ-lactone	1156:1189	GlcA degradation product γ-lactone	1156:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	3	45	theme	linckia	466:472	arg1	EPS					493:495	EPS	493:495	EPS	493:495	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	3	45	theme	linckia	466:472	arg1	exopolysaccharide					474:490	acidic lactylated Nostoc cf. linckia exopolysaccharide	437:490	acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	437:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	7	46	theme	monosaccharide	986:999	arg1	analysis					1015:1022	monosaccharide compositional analysis	986:1022	monosaccharide compositional analysis (HPLC, HPAEC)	986:1036	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	3	47	theme	NMR	519:521	arg1	spectroscopy					523:534	NMR spectroscopy	519:534	NMR spectroscopy	519:534	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	5	48	theme	monosaccharide	716:729	arg1	components					731:740	EPS monosaccharide components	712:740	EPS monosaccharide components	712:740	The stability of EPS monosaccharide components was also explored.					
34280803	6	49	theme	glucuronic	917:926	arg1	acid					928:931	glucuronic acid	917:931	glucuronic acid	917:931	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	2	50	theme	acid	241:244	arg1	hydrolysis					246:255	An acid hydrolysis	238:255	An acid hydrolysis	238:255	An acid hydrolysis is usually performed for glycosidic bonds cleavage and releasing of monosaccharides.					
34280803	3	51	dep	exopolysaccharide	474:490	arg1	cf.					462:464	cf.	462:464	cf.	462:464	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	6	52	theme	Low	761:763	arg1	stability					765:773	Low stability	761:773	Low stability	761:773	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	4	53	theme	experiments	560:570	arg1	series					550:555	a series	548:555	a series of experiments with modified acid concentration, temperature and time of hydrolysis	548:639	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	7	54	theme	compositional	1001:1013	arg1	analysis					1015:1022	monosaccharide compositional analysis	986:1022	monosaccharide compositional analysis (HPLC, HPAEC)	986:1036	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	7	55	theme	free	1098:1101	arg1	one					1103:1105	the free one	1094:1105	the free one	1094:1105	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	1	56	theme	monosaccharide	141:154	arg1	composition					156:166	monosaccharide composition	141:166	monosaccharide composition	141:166	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	2	57	theme	bonds	293:297	arg1	cleavage					299:306	glycosidic bonds cleavage	282:306	glycosidic bonds cleavage	282:306	An acid hydrolysis is usually performed for glycosidic bonds cleavage and releasing of monosaccharides.					
34280803	1	58	from	elucidation	206:216	arg1	important					171:179	important	171:179	important	171:179	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	1	58	from	elucidation	206:216	arg1	estimation					127:136	The exact estimation	117:136	The exact estimation of monosaccharide composition	117:166	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	1	59	theme	composition	156:166	arg1	important					171:179	important	171:179	important	171:179	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	1	59	theme	composition	156:166	arg1	estimation					127:136	The exact estimation	117:136	The exact estimation of monosaccharide composition	117:166	The exact estimation of monosaccharide composition is important in the primary structure elucidation of polysaccharides.					
34280803	4	60	theme	hydrolysis	630:639	arg1	time					622:625	time	622:625	time	622:625	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	60	theme	hydrolysis	630:639	arg1	temperature					606:616	temperature	606:616	temperature	606:616	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	4	60	theme	hydrolysis	630:639	arg1	concentration					591:603	modified acid concentration	577:603	modified acid concentration	577:603	Results of a series of experiments with modified acid concentration, temperature and time of hydrolysis, have shown 2 M TFA, 110 °C, 3 h as the most optimal.					
34280803	7	61	theme	degradation	1161:1171	arg1	γ-lactone					1181:1189	GlcA degradation product γ-lactone	1156:1189	GlcA degradation product γ-lactone	1156:1189	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	6	62	theme	first	829:833	arg1	hour					835:838	the first hour	825:838	the first hour of hydrolysis	825:852	Low stability was found at all tested conditions already during the first hour of hydrolysis; all neutral monosaccharides were degraded from 25% to 40% and glucuronic acid to 75%.					
34280803	2	63	theme	glycosidic	282:291	arg1	cleavage					299:306	glycosidic bonds cleavage	282:306	glycosidic bonds cleavage	282:306	An acid hydrolysis is usually performed for glycosidic bonds cleavage and releasing of monosaccharides.					
34280803	7	64	theme	standard	958:965	arg1	techniques					967:976	standard techniques	958:976	standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC)	958:1036	NMR, contrary to standard techniques used in monosaccharide compositional analysis (HPLC, HPAEC), allowed simultaneous quantification of all GlcA forms; the free one, that one linked in oligosaccharides, as well as GlcA degradation product γ-lactone.					
34280803	3	65	theme	exopolysaccharide	474:490	arg1	conditions					365:374	optimal conditions	357:374	optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS)	357:496	In this study, optimal conditions of total acid hydrolysis using trifluoroacetic acid (TFA) of acidic lactylated Nostoc cf. linckia exopolysaccharide (EPS) were investigated by NMR spectroscopy.					
34280803	0	66	theme	Nostoc	72:77	arg1	analysis					60:67	monosaccharide compositional analysis	31:67	monosaccharide compositional analysis of Nostoc	31:77	Optimizing acid hydrolysis for monosaccharide compositional analysis of Nostoc cf. linckia acidic exopolysaccharide.					
33049816	4	0	theme	composite	845:853	arg1	films					855:859	TOCNF/MF2:Ho-AKD composite films	828:859	TOCNF/MF2:Ho-AKD composite films	828:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	4	1	from	absence	760:766	arg1	atmosphere					813:822	a high humidity atmosphere	797:822	a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films	797:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	1	2	theme	TEMPO-oxidized	196:209	arg1	TOCNF					234:238	TOCNF	234:238	TOCNF	234:238	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	1	2	theme	TEMPO-oxidized	196:209	arg1	nanofibrils					221:231	TEMPO-oxidized cellulose nanofibrils	196:231	TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor	196:328	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	2	3	theme	mild	464:467	arg1	drying					469:474	mild drying	464:474	mild drying at 110 °C	464:484	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	4	4	theme	Ho-AKD	838:843	arg1	films					855:859	TOCNF/MF2:Ho-AKD composite films	828:859	TOCNF/MF2:Ho-AKD composite films	828:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	1	5	theme	cellulose	211:219	arg1	TOCNF					234:238	TOCNF	234:238	TOCNF	234:238	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	1	5	theme	cellulose	211:219	arg1	nanofibrils					221:231	TEMPO-oxidized cellulose nanofibrils	196:231	TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor	196:328	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	4	6	theme	humidity	804:811	arg1	atmosphere					813:822	a high humidity atmosphere	797:822	a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films	797:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	3	7	theme	stable	538:543	arg1	luminescence					559:570	stable up-conversion luminescence	538:570	stable up-conversion luminescence	538:570	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	8	theme	up-conversion	545:557	arg1	luminescence					559:570	stable up-conversion luminescence	538:570	stable up-conversion luminescence	538:570	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	9	theme	visible	579:585	arg1	range					596:600	the visible spectral range	575:600	the visible spectral range	575:600	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	4	10	theme	hydrophobic	926:936	arg1	matrix					948:953	the hydrophobic TOCNF-AKD matrix	922:953	the hydrophobic TOCNF-AKD matrix	922:953	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	3	11	theme	humid	719:723	arg1	atmosphere					725:734	a humid atmosphere	717:734	a humid atmosphere (92 ± 2% humidity)	717:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	11	theme	humid	719:723	arg1	humidity					745:752	92 ± 2% humidity	737:752	92 ± 2% humidity	737:752	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	2	12	theme	TOCNF/MF2	382:390	arg1	films					399:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	1	13	dep	M = Ca	304:309	arg1	Sr					312:313	Sr	312:313	Sr	312:313	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	4	14	theme	shielding	902:910	arg1	effect					912:917	the reliable shielding effect	889:917	the reliable shielding effect of the hydrophobic TOCNF-AKD matrix	889:953	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	2	15	theme	translucent	370:380	arg1	films					399:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	0	16	theme	ketene	107:112	arg1	dimer					114:118	alkyl ketene dimer	101:118	alkyl ketene dimer	101:118	Hydrophobic up-conversion carboxylated nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer.					
33049816	4	17	theme	matrix	948:953	arg1	effect					912:917	the reliable shielding effect	889:917	the reliable shielding effect of the hydrophobic TOCNF-AKD matrix	889:953	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	0	18	theme	Hydrophobic	0:10	arg1	up-conversion					12:24	Hydrophobic up-conversion	0:24	Hydrophobic up-conversion	0:24	Hydrophobic up-conversion carboxylated nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer.					
33049816	3	19	theme	92 ± 2	737:742	arg1	atmosphere					725:734	a humid atmosphere	717:734	a humid atmosphere (92 ± 2% humidity)	717:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	19	theme	92 ± 2	737:742	arg1	humidity					745:752	92 ± 2% humidity	737:752	92 ± 2% humidity	737:752	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	4	20	theme	TOCNF/MF2	828:836	arg1	films					855:859	TOCNF/MF2:Ho-AKD composite films	828:859	TOCNF/MF2:Ho-AKD composite films	828:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	1	21	theme	Hydrophobic	121:131	arg1	films					161:165	Hydrophobic up-conversion nanocomposite films	121:165	Hydrophobic up-conversion nanocomposite films	121:165	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	2	22	theme	strong	359:364	arg1	films					399:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	0	23	theme	nanocellulose/fluoride	39:60	arg1	films					81:85	nanocellulose/fluoride phosphor composite films	39:85	nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer	39:118	Hydrophobic up-conversion carboxylated nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer.					
33049816	3	24	theme	%	743:743	arg1	atmosphere					725:734	a humid atmosphere	717:734	a humid atmosphere (92 ± 2% humidity)	717:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	24	theme	%	743:743	arg1	humidity					745:752	92 ± 2% humidity	737:752	92 ± 2% humidity	737:752	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	1	25	theme	up-conversion	133:145	arg1	films					161:165	Hydrophobic up-conversion nanocomposite films	121:165	Hydrophobic up-conversion nanocomposite films	121:165	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	2	26	theme	water	410:414	arg1	angle					424:428	water contact angle	410:428	water contact angle of 123 ± 2°	410:440	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	3	27	theme	ions	643:646	arg1	level					629:633	the 5I7 level	621:633	the 5I7 level of Ho3+ ions	621:646	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	1	28	theme	nanocomposite	147:159	arg1	films					161:165	Hydrophobic up-conversion nanocomposite films	121:165	Hydrophobic up-conversion nanocomposite films	121:165	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	5	29	theme	laser	1000:1004	arg1	radiation					1006:1014	2 μm laser radiation	995:1014	2 μm laser radiation	995:1014	The films show promise for visualizing 2 μm laser radiation in medicine and monitoring of the atmosphere.					
33049816	2	30	theme	films	399:403	arg1	Fabrication					331:341	Fabrication	331:341	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2°	331:440	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	0	31	theme	composite	71:79	arg1	films					81:85	nanocellulose/fluoride phosphor composite films	39:85	nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer	39:118	Hydrophobic up-conversion carboxylated nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer.					
33049816	3	32	from	1912 nm	672:678	arg1	irradiation					657:667	laser irradiation	651:667	laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity)	651:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	32	from	1912 nm	672:678	arg1	atmosphere					725:734	a humid atmosphere	717:734	a humid atmosphere (92 ± 2% humidity)	717:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	32	from	1912 nm	672:678	arg1	humidity					745:752	92 ± 2% humidity	737:752	92 ± 2% humidity	737:752	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	2	33	theme	Ho-AKD	392:397	arg1	films					399:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	1	34	theme	alkyl	255:259	arg1	AKD					275:277	AKD	275:277	AKD	275:277	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	1	34	theme	alkyl	255:259	arg1	dimer					268:272	alkyl ketene dimer	255:272	alkyl ketene dimer (AKD)	255:278	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	0	35	theme	phosphor	62:69	arg1	films					81:85	nanocellulose/fluoride phosphor composite films	39:85	nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer	39:118	Hydrophobic up-conversion carboxylated nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer.					
33049816	4	36	theme	reliable	893:900	arg1	effect					912:917	the reliable shielding effect	889:917	the reliable shielding effect of the hydrophobic TOCNF-AKD matrix	889:953	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	2	37	from	110 °C	479:484	arg1	drying					469:474	mild drying	464:474	mild drying at 110 °C	464:484	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	2	38	theme	contact	416:422	arg1	angle					424:428	water contact angle	410:428	water contact angle of 123 ± 2°	410:440	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	1	39	theme	ketene	261:266	arg1	AKD					275:277	AKD	275:277	AKD	275:277	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	1	39	theme	ketene	261:266	arg1	dimer					268:272	alkyl ketene dimer	255:272	alkyl ketene dimer (AKD)	255:278	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	3	40	theme	Ho3+	638:641	arg1	ions					643:646	Ho3+ ions	638:646	Ho3+ ions	638:646	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	41	theme	spectral	587:594	arg1	range					596:600	the visible spectral range	575:600	the visible spectral range	575:600	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	2	42	theme	123 ± 2°	433:440	arg1	angle					424:428	water contact angle	410:428	water contact angle of 123 ± 2°	410:440	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	1	43	dep	matrix	285:290	arg1	a					283:283	a	283:283	a	283:283	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	1	43	dep	matrix	285:290	arg1	M = Ca					304:309	M = Ca	304:309	M = Ca	304:309	Hydrophobic up-conversion nanocomposite films have been developed based on TEMPO-oxidized cellulose nanofibrils (TOCNF) modified with alkyl ketene dimer (AKD) as a matrix and MF2:Ho (M = Ca, Sr) as a phosphor.					
33049816	4	44	theme	high	799:802	arg1	atmosphere					813:822	a high humidity atmosphere	797:822	a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films	797:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	5	45	theme	atmosphere	1050:1059	arg1	monitoring					1032:1041	monitoring	1032:1041	monitoring of the atmosphere	1032:1059	The films show promise for visualizing 2 μm laser radiation in medicine and monitoring of the atmosphere.					
33049816	5	45	theme	atmosphere	1050:1059	arg1	medicine					1019:1026	medicine	1019:1026	medicine	1019:1026	The films show promise for visualizing 2 μm laser radiation in medicine and monitoring of the atmosphere.					
33049816	2	46	with	Fabrication	331:341	arg1	angle					424:428	water contact angle	410:428	water contact angle of 123 ± 2°	410:440	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
33049816	3	47	theme	5I7	625:627	arg1	level					629:633	the 5I7 level	621:633	the 5I7 level of Ho3+ ions	621:646	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	5	48	theme	2 μm	995:998	arg1	radiation					1006:1014	2 μm laser radiation	995:1014	2 μm laser radiation	995:1014	The films show promise for visualizing 2 μm laser radiation in medicine and monitoring of the atmosphere.					
33049816	3	49	theme	laser	651:655	arg1	irradiation					657:667	laser irradiation	651:667	laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity)	651:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	50	theme	level	629:633	arg1	excitation					607:616	excitation	607:616	excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity)	607:753	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	3	51	theme	nanocomposite	505:517	arg1	films					519:523	These hydrophobic nanocomposite films	487:523	These hydrophobic nanocomposite films	487:523	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	0	52	theme	alkyl	101:105	arg1	dimer					114:118	alkyl ketene dimer	101:118	alkyl ketene dimer	101:118	Hydrophobic up-conversion carboxylated nanocellulose/fluoride phosphor composite films modified with alkyl ketene dimer.					
33049816	4	53	theme	quenching	784:792	arg1	absence					760:766	The absence	756:766	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films	756:859	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	4	53	theme	quenching	784:792	arg1	due					882:884	due	882:884	due	882:884	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	3	54	theme	hydrophobic	493:503	arg1	films					519:523	These hydrophobic nanocomposite films	487:523	These hydrophobic nanocomposite films	487:523	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	4	55	theme	TOCNF-AKD	938:946	arg1	matrix					948:953	the hydrophobic TOCNF-AKD matrix	922:953	the hydrophobic TOCNF-AKD matrix	922:953	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	3	56	theme	ambient	691:697	arg1	conditions					699:708	ambient conditions	691:708	ambient conditions	691:708	These hydrophobic nanocomposite films demonstrated stable up-conversion luminescence in the visible spectral range upon excitation of the 5I7 level of Ho3+ ions by laser irradiation at 1912 nm both under ambient conditions and in a humid atmosphere (92 ± 2% humidity).					
33049816	4	57	theme	luminescence	771:782	arg1	quenching					784:792	luminescence quenching	771:792	luminescence quenching	771:792	The absence of luminescence quenching in a high humidity atmosphere for TOCNF/MF2:Ho-AKD composite films was considered to be due to the reliable shielding effect of the hydrophobic TOCNF-AKD matrix.					
33049816	2	58	theme	homogeneous	346:356	arg1	films					399:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films	346:403	Fabrication of homogeneous, strong and translucent TOCNF/MF2:Ho-AKD films with water contact angle of 123 ± 2° was accomplished with mild drying at 110 °C.					
32197845	11	0	theme	milk	1959:1962	arg1	content					1972:1978	milk protein content	1959:1978	milk protein content (4.03 vs. 3.93 ± 0.10%)	1959:2002	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	0	theme	milk	1959:1962	arg1	%					2001:2001	4.03 vs. 3.93 ± 0.10%	1981:2001	%	2001:2001	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	9	1	theme	61.7	1643:1646	arg1	matter					1626:1631	dry matter	1622:1631	dry matter (66.7 vs. 61.7 ± 1.06%)	1622:1655	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	1	theme	61.7	1643:1646	arg1	%					1654:1654	66.7 vs. 61.7 ± 1.06%	1634:1654	%	1654:1654	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	2	theme	±	1648:1648	arg1	matter					1626:1631	dry matter	1622:1631	dry matter (66.7 vs. 61.7 ± 1.06%)	1622:1655	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	2	theme	±	1648:1648	arg1	%					1654:1654	66.7 vs. 61.7 ± 1.06%	1634:1654	%	1654:1654	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	2	3	theme	multiparous	332:342	arg1	mean					357:360	mean	357:360	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	3	theme	multiparous	332:342	arg1	cows					351:354	Twelve multiparous Jersey cows	325:354	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection)	325:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	5	4	theme	rumen-inert	960:970	arg1	source					976:981	a rumen-inert fat source	958:981	a rumen-inert fat source	958:981	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	7	5	theme	1.01	1405:1408	arg1	Mcal/d					1410:1415	4.70 vs. 2.14 ± 1.01 Mcal/d	1389:1415	4.70 vs. 2.14 ± 1.01 Mcal/d	1389:1415	Tissue energy deposited as body fat tended to be greater for HS (4.70 vs. 2.14 ± 1.01 Mcal/d).					
32197845	8	6	theme	N	1457:1457	arg1	g/d					1489:1491	141 vs. 131 ± 10.5 g/d	1470:1491	141 vs. 131 ± 10.5 g/d	1470:1491	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	8	6	theme	N	1457:1457	arg1	secretion					1459:1467	milk N secretion	1452:1467	milk N secretion (141 vs. 131 ± 10.5 g/d)	1452:1492	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	12	7	theme	milk	2262:2265	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (average of 27.2 ± 1.99 kg/d)	2245:2301	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	7	theme	milk	2262:2265	arg1	kg/d					2297:2300	average of 27.2 ± 1.99 kg/d	2274:2300	average of 27.2 ± 1.99 kg/d	2274:2300	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	3	8	dep	%	571:571	arg1	starch					573:578	starch	573:578	30.8% starch	567:578	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	12	9	theme	milk	2197:2200	arg1	yield					2206:2210	milk fat yield	2197:2210	milk fat yield (average of 1.19 ± 0.09 kg/d)	2197:2240	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	11	10	theme	0.050	2043:2047	arg1	kg/d					2049:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	10	theme	0.050	2043:2047	arg1	yield					2018:2022	milk protein yield	2005:2022	milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d)	2005:2053	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	3	11	theme	neutral	587:593	arg1	fiber					605:609	31.8% neutral detergent fiber	581:609	31.8% neutral detergent fiber	581:609	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	6	12	theme	Gross	1011:1015	arg1	content					1024:1030	Gross energy content	1011:1030	Gross energy content	1011:1030	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	6	13	theme	dry	1219:1221	arg1	matter					1223:1228	dry matter	1219:1228	dry matter	1219:1228	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	9	14	theme	matter	1666:1671	arg1	digestibility					1605:1617	apparent total-tract digestibility	1584:1617	apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1584:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	12	15	theme	kg/d	2236:2239	arg1	average					2213:2219	average	2213:2219	average of 1.19 ± 0.09 kg/d	2213:2239	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	2	16	from	cows	351:354	arg1	design					432:437	a crossover design	420:437	a crossover design	420:437	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	9	17	theme	68.5	1674:1677	arg1	matter					1666:1671	organic matter	1658:1671	organic matter (68.5 vs. 63.2 ± 0.98%)	1658:1695	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	17	theme	68.5	1674:1677	arg1	%					1694:1694	68.5 vs. 63.2 ± 0.98%	1674:1694	%	1694:1694	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	5	18	from	source	976:981	arg1	HF					1007:1008	HF	1007:1008	HF	1007:1008	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	3	19	dep	fat	642:644	arg1	HF					647:648	HF	647:648	HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids	647:715	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	9	20	theme	±	1688:1688	arg1	matter					1666:1671	organic matter	1658:1671	organic matter (68.5 vs. 63.2 ± 0.98%)	1658:1695	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	20	theme	±	1688:1688	arg1	%					1694:1694	68.5 vs. 63.2 ± 0.98%	1674:1694	%	1694:1694	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	2	21	theme	24-d	458:461	arg1	adaptation					463:472	24-d adaptation	458:472	24-d adaptation	458:472	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	21	theme	24-d	458:461	arg1	periods					449:455	28-d periods	444:455	28-d periods (24-d adaptation and 4-d collection)	444:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	11	22	theme	milk	1919:1922	arg1	kg/d					1952:1955	19.7 vs. 18.9 ± 1.38 kg/d	1931:1955	19.7 vs. 18.9 ± 1.38 kg/d	1931:1955	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	22	theme	milk	1919:1922	arg1	yield					1924:1928	milk yield	1919:1928	milk yield (19.7 vs. 18.9 ± 1.38 kg/d)	1919:1956	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	13	23	theme	metabolizable	2372:2384	arg1	energy					2386:2391	a greater metabolizable energy and NEL content	2362:2407	energy	2386:2391	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	2	24	used	used	499:502	arg2	mean					357:360	mean	357:360	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	24	used	used	499:502	arg2	cows					351:354	Twelve multiparous Jersey cows	325:354	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection)	325:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	0	25	theme	lactating	126:134	arg1	cows					143:146	lactating Jersey cows	126:146	lactating Jersey cows	126:146	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	3	26	dep	HF	647:648	arg1	%					655:655	16.8% starch	651:662	16.8% starch	651:662	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	26	dep	HF	647:648	arg1	%					703:703	4.1%	700:703	4.1% fatty acids	700:715	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	26	dep	HF	647:648	arg1	%					669:669	41.7%	665:669	41.7% neutral detergent fiber	665:693	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	27	dep	%	703:703	arg1	acids					711:715	fatty acids	705:715	4.1% fatty acids	700:715	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	11	28	theme	milk	2005:2008	arg1	kg/d					2049:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	28	theme	milk	2005:2008	arg1	yield					2018:2022	milk protein yield	2005:2022	milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d)	2005:2053	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	12	29	theme	±	2229:2229	arg1	kg/d					2236:2239	1.19 ± 0.09 kg/d	2224:2239	1.19 ± 0.09 kg/d	2224:2239	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	3	30	theme	neutral	671:677	arg1	fiber					689:693	neutral detergent fiber	671:693	41.7% neutral detergent fiber	665:693	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	0	31	from	partitioning	94:105	arg1	cows					143:146	lactating Jersey cows	126:146	lactating Jersey cows	126:146	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	8	32	theme	urinary	1508:1514	arg1	excretion					1518:1526	urinary N excretion	1508:1526	urinary N excretion (123 vs. 150 ± 6.4 g/d)	1508:1550	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	8	32	theme	urinary	1508:1514	arg1	g/d					1547:1549	123 vs. 150 ± 6.4 g/d	1529:1549	123 vs. 150 ± 6.4 g/d	1529:1549	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	12	33	theme	fat	2202:2204	arg1	yield					2206:2210	milk fat yield	2197:2210	milk fat yield (average of 1.19 ± 0.09 kg/d)	2197:2240	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	4	34	theme	lactation	764:772	arg1	content					780:786	lactation (NEL) content	764:786	lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model	764:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	9	35	theme	energy	1698:1703	arg1	digestibility					1605:1617	apparent total-tract digestibility	1584:1617	apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1584:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	6	36	theme	digestible	1103:1112	arg1	energy					1178:1183	digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy	1103:1183	digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter)	1103:1229	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	5	37	theme	Nutrient	881:888	arg1	composition					890:900	Nutrient composition	881:900	Nutrient composition	881:900	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	7	38	theme	body	1351:1354	arg1	fat					1356:1358	body fat	1351:1358	body fat	1351:1358	Tissue energy deposited as body fat tended to be greater for HS (4.70 vs. 2.14 ± 1.01 Mcal/d).					
32197845	4	39	theme	NEL	775:777	arg1	content					780:786	lactation (NEL) content	764:786	lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model	764:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	2	40	from	d	393:393	arg1	milk					398:401	milk	398:401	milk	398:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	9	41	theme	66.0	1706:1709	arg1	energy					1698:1703	energy	1698:1703	energy (66.0 vs. 60.4 ± 0.92%)	1698:1727	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	41	theme	66.0	1706:1709	arg1	%					1726:1726	66.0 vs. 60.4 ± 0.92%	1706:1726	%	1726:1726	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	3	42	dep	%	619:619	arg1	acids					627:631	fatty acids	621:631	1.9% fatty acids	616:631	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	13	43	theme	energy	2532:2537	arg1	partitioning					2516:2527	increased partitioning	2506:2527	increased partitioning of energy toward tissue energy deposited as fat	2506:2575	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	10	44	theme	starch	1828:1833	arg1	digestibility					1811:1823	apparent total-tract digestibility	1790:1823	apparent total-tract digestibility of starch	1790:1833	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	9	45	theme	60.4	1715:1718	arg1	energy					1698:1703	energy	1698:1703	energy (66.0 vs. 60.4 ± 0.92%)	1698:1727	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	45	theme	60.4	1715:1718	arg1	%					1726:1726	66.0 vs. 60.4 ± 0.92%	1706:1726	%	1726:1726	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	6	46	theme	matter	1086:1091	arg1	Mcal/kg					1071:1077	4.43 vs. 4.54 ± 0.01 Mcal/kg	1050:1077	4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter	1050:1091	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	13	47	theme	tissue	2546:2551	arg1	energy					2553:2558	tissue energy	2546:2558	tissue energy deposited as fat	2546:2575	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	12	48	theme	±	2290:2290	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (average of 27.2 ± 1.99 kg/d)	2245:2301	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	48	theme	±	2290:2290	arg1	kg/d					2297:2300	average of 27.2 ± 1.99 kg/d	2274:2300	average of 27.2 ± 1.99 kg/d	2274:2300	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	7	49	theme	Tissue	1324:1329	arg1	energy					1331:1336	Tissue energy	1324:1336	Tissue energy deposited as body fat	1324:1358	Tissue energy deposited as body fat tended to be greater for HS (4.70 vs. 2.14 ± 1.01 Mcal/d).					
32197845	13	50	contain	had	2358:2360	arg2	content					2401:2407	a greater metabolizable energy and NEL content	2362:2407	content	2401:2407	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	13	50	contain	had	2358:2360	arg1	diet					2353:2356	the HS diet	2346:2356	the HS diet	2346:2356	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	13	50	contain	had	2358:2360	arg2	energy					2386:2391	a greater metabolizable energy and NEL content	2362:2407	energy	2386:2391	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	7	51	dep	greater	1373:1379	arg1	Mcal/d					1410:1415	4.70 vs. 2.14 ± 1.01 Mcal/d	1389:1415	4.70 vs. 2.14 ± 1.01 Mcal/d	1389:1415	Tissue energy deposited as body fat tended to be greater for HS (4.70 vs. 2.14 ± 1.01 Mcal/d).					
32197845	6	52	theme	dry	1274:1276	arg1	matter					1278:1283	dry matter	1274:1283	dry matter	1274:1283	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	2	53	theme	crossover	422:430	arg1	design					432:437	a crossover design	420:437	a crossover design	420:437	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	54	theme	±	362:362	arg1	deviation					373:381	± standard deviation	362:381	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	6	55	dep	digestible	1103:1112	arg1	Mcal/kg					1137:1143	2.93 vs. 2.74 ± 0.035 Mcal/kg	1115:1143	2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter	1115:1157	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	4	56	dep	Council	853:859	arg1	model					874:878	dairy model	868:878	the National Research Council (2001) dairy model	831:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	13	57	theme	HS	2350:2351	arg1	diet					2353:2356	the HS diet	2346:2356	the HS diet	2346:2356	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	4	58	theme	matter	811:816	arg1	Mcal/kg					796:802	1.55 Mcal/kg	791:802	1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model	791:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	12	59	theme	fat	2142:2144	arg1	content					2146:2152	milk fat content	2137:2152	milk fat content (5.93 vs. 6.37 ± 0.15%)	2137:2176	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	59	theme	fat	2142:2144	arg1	%					2175:2175	5.93 vs. 6.37 ± 0.15%	2155:2175	%	2175:2175	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	0	60	theme	high-starch	11:21	arg1	diets					35:39	high-starch or high-fat diets	11:39	high-starch or high-fat diets	11:39	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	9	61	theme	total-tract	1593:1603	arg1	digestibility					1605:1617	apparent total-tract digestibility	1584:1617	apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1584:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	62	theme	fatty	1744:1748	arg1	%					1777:1777	67.9 vs. 61.2 ± 1.60%	1757:1777	%	1777:1777	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	62	theme	fatty	1744:1748	arg1	acids					1750:1754	18-carbon fatty acids	1734:1754	18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1734:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	0	63	theme	high-fat	26:33	arg1	diets					35:39	high-starch or high-fat diets	11:39	high-starch or high-fat diets	11:39	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	1	64	theme	N	283:283	arg1	partitioning					285:296	N partitioning	283:296	N partitioning	283:296	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	13	65	theme	study	2327:2331	arg1	Results					2304:2310	Results	2304:2310	Results of the current study	2304:2331	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	2	66	theme	±	408:408	arg1	kg					413:414	467 ± 47 kg	404:414	467 ± 47 kg	404:414	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	3	67	theme	high	550:553	arg1	starch					555:560	high starch	550:560	high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids)	550:632	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	11	68	theme	lactose	2065:2071	arg1	kg/d					2104:2107	0.897 vs. 0.864 ± 0.067 kg/d	2080:2107	0.897 vs. 0.864 ± 0.067 kg/d	2080:2107	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	68	theme	lactose	2065:2071	arg1	yield					2073:2077	milk lactose yield	2060:2077	milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d)	2060:2108	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	69	theme	±	1995:1995	arg1	content					1972:1978	milk protein content	1959:1978	milk protein content (4.03 vs. 3.93 ± 0.10%)	1959:2002	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	69	theme	±	1995:1995	arg1	%					2001:2001	4.03 vs. 3.93 ± 0.10%	1981:2001	%	2001:2001	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	9	70	theme	matter	1626:1631	arg1	digestibility					1605:1617	apparent total-tract digestibility	1584:1617	apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1584:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	12	71	theme	±	2169:2169	arg1	content					2146:2152	milk fat content	2137:2152	milk fat content (5.93 vs. 6.37 ± 0.15%)	2137:2176	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	71	theme	±	2169:2169	arg1	%					2175:2175	5.93 vs. 6.37 ± 0.15%	2155:2175	%	2175:2175	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	9	72	theme	66.7	1634:1637	arg1	matter					1626:1631	dry matter	1622:1631	dry matter (66.7 vs. 61.7 ± 1.06%)	1622:1655	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	72	theme	66.7	1634:1637	arg1	%					1654:1654	66.7 vs. 61.7 ± 1.06%	1634:1654	%	1654:1654	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	73	theme	±	1771:1771	arg1	%					1777:1777	67.9 vs. 61.2 ± 1.60%	1757:1777	%	1777:1777	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	73	theme	±	1771:1771	arg1	acids					1750:1754	18-carbon fatty acids	1734:1754	18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1734:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	3	74	dep	starch	555:560	arg1	HS					563:564	HS	563:564	HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids	563:631	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	0	75	from	utilization	111:121	arg1	cows					143:146	lactating Jersey cows	126:146	lactating Jersey cows	126:146	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	3	76	theme	%	585:585	arg1	fiber					605:609	31.8% neutral detergent fiber	581:609	31.8% neutral detergent fiber	581:609	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	8	77	theme	milk	1452:1455	arg1	g/d					1489:1491	141 vs. 131 ± 10.5 g/d	1470:1491	141 vs. 131 ± 10.5 g/d	1470:1491	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	8	77	theme	milk	1452:1455	arg1	secretion					1459:1467	milk N secretion	1452:1467	milk N secretion (141 vs. 131 ± 10.5 g/d)	1452:1492	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	9	78	theme	organic	1658:1664	arg1	matter					1666:1671	organic matter	1658:1671	organic matter (68.5 vs. 63.2 ± 0.98%)	1658:1695	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	78	theme	organic	1658:1664	arg1	%					1694:1694	68.5 vs. 63.2 ± 0.98%	1674:1694	%	1694:1694	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	2	79	theme	Jersey	344:349	arg1	mean					357:360	mean	357:360	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	79	theme	Jersey	344:349	arg1	cows					351:354	Twelve multiparous Jersey cows	325:354	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection)	325:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	1	80	theme	diets	233:237	arg1	effects					198:204	the effects	194:204	the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy	194:322	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	7	81	theme	±	1403:1403	arg1	Mcal/d					1410:1415	4.70 vs. 2.14 ± 1.01 Mcal/d	1389:1415	4.70 vs. 2.14 ± 1.01 Mcal/d	1389:1415	Tissue energy deposited as body fat tended to be greater for HS (4.70 vs. 2.14 ± 1.01 Mcal/d).					
32197845	4	82	theme	National	835:842	arg1	Council					853:859	the National Research Council	831:859	the National Research Council (2001) dairy model	831:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	4	82	theme	National	835:842	arg1	2001					862:865	2001	862:865	2001	862:865	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	11	83	theme	protein	1964:1970	arg1	content					1972:1978	milk protein content	1959:1978	milk protein content (4.03 vs. 3.93 ± 0.10%)	1959:2002	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	83	theme	protein	1964:1970	arg1	%					2001:2001	4.03 vs. 3.93 ± 0.10%	1981:2001	%	2001:2001	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	6	84	theme	matter	1152:1157	arg1	Mcal/kg					1137:1143	2.93 vs. 2.74 ± 0.035 Mcal/kg	1115:1143	2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter	1115:1157	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	5	85	theme	fat	972:974	arg1	source					976:981	a rumen-inert fat source	958:981	a rumen-inert fat source	958:981	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	13	86	theme	N	2436:2436	arg1	partitioning					2420:2431	partitioning	2420:2431	partitioning of N toward milk secretion and away from urinary excretion	2420:2490	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	13	87	from	excretion	2482:2490	arg1	partitioning					2420:2431	partitioning	2420:2431	partitioning of N toward milk secretion and away from urinary excretion	2420:2490	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	12	88	theme	of	2282:2283	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (average of 27.2 ± 1.99 kg/d)	2245:2301	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	88	theme	of	2282:2283	arg1	kg/d					2297:2300	average of 27.2 ± 1.99 kg/d	2274:2300	average of 27.2 ± 1.99 kg/d	2274:2300	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	6	89	theme	energy	1017:1022	arg1	content					1024:1030	Gross energy content	1011:1030	Gross energy content	1011:1030	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	12	90	theme	average	2274:2280	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (average of 27.2 ± 1.99 kg/d)	2245:2301	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	90	theme	average	2274:2280	arg1	kg/d					2297:2300	average of 27.2 ± 1.99 kg/d	2274:2300	average of 27.2 ± 1.99 kg/d	2274:2300	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	6	91	theme	matter	1223:1228	arg1	Mcal/kg					1208:1214	2.60 vs. 2.41 ± 0.030 Mcal/kg	1186:1214	2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter	1186:1228	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	13	92	theme	milk	2445:2448	arg1	secretion					2450:2458	milk secretion	2445:2458	milk secretion	2445:2458	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	3	93	theme	detergent	595:603	arg1	fiber					605:609	31.8% neutral detergent fiber	581:609	31.8% neutral detergent fiber	581:609	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	5	94	from	grain	941:945	arg1	HS					950:951	HS	950:951	HS	950:951	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	1	95	theme	study	171:175	arg1	objective					153:161	The objective	149:161	The objective of this study	149:175	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	6	96	dep	HS	1046:1047	arg1	Mcal/kg					1071:1077	4.43 vs. 4.54 ± 0.01 Mcal/kg	1050:1077	4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter	1050:1091	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	2	97	theme	treatment	517:525	arg1	diets					527:531	2 treatment diets	515:531	2 treatment diets	515:531	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	4	98	theme	dairy	868:872	arg1	model					874:878	dairy model	868:878	the National Research Council (2001) dairy model	831:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	10	99	theme	0.48	1872:1875	arg1	±					1870:1870	±	1870:1870	±	1870:1870	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	3	100	theme	fatty	621:625	arg1	acids					627:631	fatty acids	621:631	1.9% fatty acids	616:631	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	12	101	theme	energy-corrected	2245:2260	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (average of 27.2 ± 1.99 kg/d)	2245:2301	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	101	theme	energy-corrected	2245:2260	arg1	kg/d					2297:2300	average of 27.2 ± 1.99 kg/d	2274:2300	average of 27.2 ± 1.99 kg/d	2274:2300	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	11	102	theme	milk	2060:2063	arg1	kg/d					2104:2107	0.897 vs. 0.864 ± 0.067 kg/d	2080:2107	0.897 vs. 0.864 ± 0.067 kg/d	2080:2107	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	102	theme	milk	2060:2063	arg1	yield					2073:2077	milk lactose yield	2060:2077	milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d)	2060:2108	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	12	103	theme	5.93	2155:2158	arg1	content					2146:2152	milk fat content	2137:2152	milk fat content (5.93 vs. 6.37 ± 0.15%)	2137:2176	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	103	theme	5.93	2155:2158	arg1	%					2175:2175	5.93 vs. 6.37 ± 0.15%	2155:2175	%	2175:2175	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	0	104	theme	Jersey	136:141	arg1	cows					143:146	lactating Jersey cows	126:146	lactating Jersey cows	126:146	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	12	105	theme	1.19	2224:2227	arg1	kg/d					2236:2239	1.19 ± 0.09 kg/d	2224:2239	1.19 ± 0.09 kg/d	2224:2239	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	11	106	theme	±	2041:2041	arg1	kg/d					2049:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	106	theme	±	2041:2041	arg1	yield					2018:2022	milk protein yield	2005:2022	milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d)	2005:2053	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	3	107	dep	HS	563:564	arg1	%					619:619	1.9%	616:619	1.9% fatty acids	616:631	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	107	dep	HS	563:564	arg1	fiber					605:609	31.8% neutral detergent fiber	581:609	31.8% neutral detergent fiber	581:609	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	107	dep	HS	563:564	arg1	%					571:571	30.8% starch	567:578	30.8% starch	567:578	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	108	theme	high	637:640	arg1	fat					642:644	high fat	637:644	high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids)	637:716	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	5	109	from	hulls	998:1002	arg1	HF					1007:1008	HF	1007:1008	HF	1007:1008	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	13	110	theme	NEL	2397:2399	arg1	content					2401:2407	a greater metabolizable energy and NEL content	2362:2407	content	2401:2407	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	11	111	theme	protein	2010:2016	arg1	kg/d					2049:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	0.791 vs. 0.740 ± 0.050 kg/d	2025:2052	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	111	theme	protein	2010:2016	arg1	yield					2018:2022	milk protein yield	2005:2022	milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d)	2005:2053	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	8	112	theme	N	1516:1516	arg1	excretion					1518:1526	urinary N excretion	1508:1526	urinary N excretion (123 vs. 150 ± 6.4 g/d)	1508:1550	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	8	112	theme	N	1516:1516	arg1	g/d					1547:1549	123 vs. 150 ± 6.4 g/d	1529:1549	123 vs. 150 ± 6.4 g/d	1529:1549	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	3	113	dep	%	655:655	arg1	starch					657:662	starch	657:662	16.8% starch	651:662	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	3	114	theme	detergent	679:687	arg1	fiber					689:693	neutral detergent fiber	671:693	41.7% neutral detergent fiber	665:693	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	2	115	theme	4-d	478:480	arg1	collection					482:491	4-d collection	478:491	4-d collection	478:491	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	115	theme	4-d	478:480	arg1	periods					449:455	28-d periods	444:455	28-d periods (24-d adaptation and 4-d collection)	444:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	12	116	theme	6.37	2164:2167	arg1	content					2146:2152	milk fat content	2137:2152	milk fat content (5.93 vs. 6.37 ± 0.15%)	2137:2176	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	116	theme	6.37	2164:2167	arg1	%					2175:2175	5.93 vs. 6.37 ± 0.15%	2155:2175	%	2175:2175	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	6	117	dep	NEL	1236:1238	arg1	Mcal/kg					1263:1269	1.83 vs. 1.67 ± 0.036 Mcal/kg	1241:1269	1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter	1241:1283	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	13	118	theme	greater	2364:2370	arg1	energy					2386:2391	a greater metabolizable energy and NEL content	2362:2407	energy	2386:2391	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	0	119	theme	energy	74:79	arg1	partitioning					94:105	energy and nitrogen partitioning	74:105	partitioning	94:105	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	1	120	theme	energy	317:322	arg1	energy					272:277	energy	272:277	energy	272:277	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	1	120	theme	energy	317:322	arg1	partitioning					285:296	N partitioning	283:296	N partitioning	283:296	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	1	120	theme	energy	317:322	arg1	utilization					302:312	utilization	302:312	utilization of energy	302:322	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	13	121	contain	have	2501:2504	arg1	diet					2353:2356	the HS diet	2346:2356	the HS diet	2346:2356	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	13	121	contain	have	2501:2504	arg2	partitioning					2516:2527	increased partitioning	2506:2527	increased partitioning of energy toward tissue energy deposited as fat	2506:2575	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	10	122	theme	apparent	1790:1797	arg1	digestibility					1811:1823	apparent total-tract digestibility	1790:1823	apparent total-tract digestibility of starch	1790:1833	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	10	123	theme	total-tract	1799:1809	arg1	digestibility					1811:1823	apparent total-tract digestibility	1790:1823	apparent total-tract digestibility of starch	1790:1833	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	0	124	theme	nitrogen	85:92	arg1	partitioning					94:105	energy and nitrogen partitioning	74:105	partitioning	94:105	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	10	125	dep	±	1870:1870	arg1	to					1862:1863	to	1862:1863	to	1862:1863	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	3	126	dep	%	669:669	arg1	fiber					689:693	neutral detergent fiber	671:693	41.7% neutral detergent fiber	665:693	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	2	127	theme	28-d	444:447	arg1	adaptation					463:472	24-d adaptation	458:472	24-d adaptation	458:472	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	127	theme	28-d	444:447	arg1	collection					482:491	4-d collection	478:491	4-d collection	478:491	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	127	theme	28-d	444:447	arg1	periods					449:455	28-d periods	444:455	28-d periods (24-d adaptation and 4-d collection)	444:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	8	128	theme	±	1541:1541	arg1	excretion					1518:1526	urinary N excretion	1508:1526	urinary N excretion (123 vs. 150 ± 6.4 g/d)	1508:1550	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	8	128	theme	±	1541:1541	arg1	g/d					1547:1549	123 vs. 150 ± 6.4 g/d	1529:1549	123 vs. 150 ± 6.4 g/d	1529:1549	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	9	129	theme	±	1720:1720	arg1	energy					1698:1703	energy	1698:1703	energy (66.0 vs. 60.4 ± 0.92%)	1698:1727	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	129	theme	±	1720:1720	arg1	%					1726:1726	66.0 vs. 60.4 ± 0.92%	1706:1726	%	1726:1726	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	6	130	theme	dry	1082:1084	arg1	matter					1086:1091	dry matter	1082:1091	dry matter	1082:1091	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	6	131	theme	matter	1278:1283	arg1	Mcal/kg					1263:1269	1.83 vs. 1.67 ± 0.036 Mcal/kg	1241:1269	1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter	1241:1283	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	4	132	theme	net	749:751	arg1	energy					753:758	net energy	749:758	net energy	749:758	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	1	133	theme	high-starch	209:219	arg1	diets					233:237	high-starch or high-fat diets	209:237	high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy	209:322	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
32197845	2	134	theme	standard	364:371	arg1	deviation					373:381	± standard deviation	362:381	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	6	135	theme	dry	1148:1150	arg1	matter					1152:1157	dry matter	1148:1157	dry matter	1148:1157	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	13	136	theme	increased	2506:2514	arg1	partitioning					2516:2527	increased partitioning	2506:2527	increased partitioning of energy toward tissue energy deposited as fat	2506:2575	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	8	137	theme	N	1423:1423	arg1	partitioning					1425:1436	N partitioning	1423:1436	N partitioning	1423:1436	For N partitioning, HS increased milk N secretion (141 vs. 131 ± 10.5 g/d) and decreased urinary N excretion (123 vs. 150 ± 6.4 g/d).					
32197845	3	138	theme	fatty	705:709	arg1	acids					711:715	fatty acids	705:715	4.1% fatty acids	700:715	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	12	139	dep	yield	2206:2210	arg1	average					2213:2219	average	2213:2219	average of 1.19 ± 0.09 kg/d	2213:2239	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	4	140	theme	Mcal/kg	796:802	arg1	content					780:786	lactation (NEL) content	764:786	lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model	764:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	11	141	theme	4.03	1981:1984	arg1	content					1972:1978	milk protein content	1959:1978	milk protein content (4.03 vs. 3.93 ± 0.10%)	1959:2002	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	141	theme	4.03	1981:1984	arg1	%					2001:2001	4.03 vs. 3.93 ± 0.10%	1981:2001	%	2001:2001	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	13	142	theme	urinary	2474:2480	arg1	excretion					2482:2490	urinary excretion	2474:2490	urinary excretion	2474:2490	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	4	143	theme	dry	807:809	arg1	matter					811:816	dry matter	807:816	dry matter according to the National Research Council (2001) dairy model	807:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	9	144	theme	apparent	1584:1591	arg1	digestibility					1605:1617	apparent total-tract digestibility	1584:1617	apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1584:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	2	145	theme	±	388:388	arg1	d					393:393	192 ± 11 d	384:393	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	0	146	theme	diets	35:39	arg1	Effects					0:6	Effects	0:6	Effects of high-starch or high-fat diets	0:39	Effects of high-starch or high-fat diets formulated to be isoenergetic on energy and nitrogen partitioning and utilization in lactating Jersey cows.					
32197845	2	147	dep	periods	449:455	arg1	adaptation					463:472	24-d adaptation	458:472	24-d adaptation	458:472	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	147	dep	periods	449:455	arg1	collection					482:491	4-d collection	478:491	4-d collection	478:491	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	147	dep	periods	449:455	arg1	periods					449:455	28-d periods	444:455	28-d periods (24-d adaptation and 4-d collection)	444:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	13	148	theme	current	2319:2325	arg1	study					2327:2331	the current study	2315:2331	the current study	2315:2331	Results of the current study suggest that the HS diet had a greater metabolizable energy and NEL content, increased partitioning of N toward milk secretion and away from urinary excretion, and may have increased partitioning of energy toward tissue energy deposited as fat.					
32197845	10	149	theme	94.5	1865:1868	arg1	±					1870:1870	±	1870:1870	±	1870:1870	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	9	150	theme	acids	1750:1754	arg1	digestibility					1605:1617	apparent total-tract digestibility	1584:1617	apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1584:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	151	theme	63.2	1683:1686	arg1	matter					1666:1671	organic matter	1658:1671	organic matter (68.5 vs. 63.2 ± 0.98%)	1658:1695	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	151	theme	63.2	1683:1686	arg1	%					1694:1694	68.5 vs. 63.2 ± 0.98%	1674:1694	%	1694:1694	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	10	152	theme	97.0	1857:1860	arg1	±					1870:1870	±	1870:1870	±	1870:1870	However, apparent total-tract digestibility of starch decreased for HS from 97.0 to 94.5 ± 0.48%.					
32197845	11	153	theme	3.93	1990:1993	arg1	content					1972:1978	milk protein content	1959:1978	milk protein content (4.03 vs. 3.93 ± 0.10%)	1959:2002	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	11	153	theme	3.93	1990:1993	arg1	%					2001:2001	4.03 vs. 3.93 ± 0.10%	1981:2001	%	2001:2001	Compared with HF, HS tended to increase milk yield (19.7 vs. 18.9 ± 1.38 kg/d), milk protein content (4.03 vs. 3.93 ± 0.10%), milk protein yield (0.791 vs. 0.740 ± 0.050 kg/d), and milk lactose yield (0.897 vs. 0.864 ± 0.067 kg/d).					
32197845	9	154	theme	dry	1622:1624	arg1	matter					1626:1631	dry matter	1622:1631	dry matter (66.7 vs. 61.7 ± 1.06%)	1622:1655	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	154	theme	dry	1622:1624	arg1	%					1654:1654	66.7 vs. 61.7 ± 1.06%	1634:1654	%	1654:1654	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	155	theme	67.9	1757:1760	arg1	%					1777:1777	67.9 vs. 61.2 ± 1.60%	1757:1777	%	1777:1777	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	155	theme	67.9	1757:1760	arg1	acids					1750:1754	18-carbon fatty acids	1734:1754	18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1734:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	5	156	theme	corn	936:939	arg1	grain					941:945	corn grain	936:945	corn grain in HS	936:951	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	4	157	contain	have	744:747	arg1	Diets					719:723	Diets	719:723	Diets	719:723	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	4	157	contain	have	744:747	arg2	energy					753:758	net energy	749:758	net energy	749:758	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	4	158	theme	Research	844:851	arg1	Council					853:859	the National Research Council	831:859	the National Research Council (2001) dairy model	831:878	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	4	158	theme	Research	844:851	arg1	2001					862:865	2001	862:865	2001	862:865	Diets were formulated to have net energy for lactation (NEL) content of 1.55 Mcal/kg of dry matter according to the National Research Council (2001) dairy model.					
32197845	9	159	theme	18-carbon	1734:1742	arg1	%					1777:1777	67.9 vs. 61.2 ± 1.60%	1757:1777	%	1777:1777	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	159	theme	18-carbon	1734:1742	arg1	acids					1750:1754	18-carbon fatty acids	1734:1754	18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1734:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	2	160	dep	mean	357:360	arg1	d					393:393	192 ± 11 d	384:393	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	160	dep	mean	357:360	arg1	deviation					373:381	± standard deviation	362:381	mean ± standard deviation; 192 ± 11 d in milk	357:401	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	160	dep	mean	357:360	arg1	kg					413:414	467 ± 47 kg	404:414	467 ± 47 kg	404:414	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	9	161	theme	61.2	1766:1769	arg1	%					1777:1777	67.9 vs. 61.2 ± 1.60%	1757:1777	%	1777:1777	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	9	161	theme	61.2	1766:1769	arg1	acids					1750:1754	18-carbon fatty acids	1734:1754	18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%)	1734:1778	Compared with HF, HS increased apparent total-tract digestibility of dry matter (66.7 vs. 61.7 ± 1.06%), organic matter (68.5 vs. 63.2 ± 0.98%), energy (66.0 vs. 60.4 ± 0.92%), and 18-carbon fatty acids (67.9 vs. 61.2 ± 1.60%).					
32197845	5	162	theme	cottonseed	987:996	arg1	hulls					998:1002	cottonseed hulls	987:1002	cottonseed hulls in HF	987:1008	Nutrient composition was varied primarily by replacing corn grain in HS with a rumen-inert fat source and cottonseed hulls in HF.					
32197845	2	163	with	cows	351:354	arg1	adaptation					463:472	24-d adaptation	458:472	24-d adaptation	458:472	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	163	with	cows	351:354	arg1	collection					482:491	4-d collection	478:491	4-d collection	478:491	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	2	163	with	cows	351:354	arg1	periods					449:455	28-d periods	444:455	28-d periods (24-d adaptation and 4-d collection)	444:492	Twelve multiparous Jersey cows (mean ± standard deviation; 192 ± 11 d in milk; 467 ± 47 kg) in a crossover design with 28-d periods (24-d adaptation and 4-d collection) were used to compare 2 treatment diets.					
32197845	6	164	dep	energy	1178:1183	arg1	Mcal/kg					1208:1214	2.60 vs. 2.41 ± 0.030 Mcal/kg	1186:1214	2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter	1186:1228	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	6	164	dep	energy	1178:1183	arg1	content					1286:1292	content	1286:1292	content	1286:1292	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	3	165	theme	31.8	581:584	arg1	%					585:585	%	585:585	%	585:585	Treatments were high starch (HS; 30.8% starch, 31.8% neutral detergent fiber, and 1.9% fatty acids) or high fat (HF; 16.8% starch, 41.7% neutral detergent fiber, and 4.1% fatty acids).					
32197845	12	166	theme	milk	2137:2140	arg1	content					2146:2152	milk fat content	2137:2152	milk fat content (5.93 vs. 6.37 ± 0.15%)	2137:2176	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	12	166	theme	milk	2137:2140	arg1	%					2175:2175	5.93 vs. 6.37 ± 0.15%	2155:2175	%	2175:2175	In addition, HS decreased milk fat content (5.93 vs. 6.37 ± 0.15%) but did not affect milk fat yield (average of 1.19 ± 0.09 kg/d) or energy-corrected milk yield (average of 27.2 ± 1.99 kg/d).					
32197845	6	167	theme	metabolizable	1164:1176	arg1	energy					1178:1183	digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy	1103:1183	digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter)	1103:1229	Gross energy content was lower for HS (4.43 vs. 4.54 ± 0.01 Mcal/kg of dry matter), whereas digestible (2.93 vs. 2.74 ± 0.035 Mcal/kg of dry matter) and metabolizable energy (2.60 vs. 2.41 ± 0.030 Mcal/kg of dry matter), and NEL (1.83 vs. 1.67 ± 0.036 Mcal/kg of dry matter) content were all greater than for HF.					
32197845	1	168	theme	high-fat	224:231	arg1	diets					233:237	high-starch or high-fat diets	209:237	high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy	209:322	The objective of this study was to determine the effects of high-starch or high-fat diets formulated to be isoenergetic on energy and N partitioning and utilization of energy.					
33872611	0	0	theme	Facile	109:114	arg1	preparation					116:126	Rhodamine B. Facile preparation	96:126	Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	96:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	2	1	with	catalyst	695:702	arg1	decomposition					818:830	almost complete decomposition	802:830	almost complete decomposition occurring within 180 min	802:855	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	1	2	theme	exchange	578:585	arg1	reaction					587:594	mixed cyclic anhydrides and ion exchange reaction	546:594	reaction	587:594	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	2	3	theme	Fenton-like	683:693	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	3	theme	Fenton-like	683:693	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	3	4	theme	green	1096:1100	arg1	catalysis					1102:1110	green catalysis	1096:1110	green catalysis	1096:1110	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	1	5	theme	cellulose	461:469	arg1	solution					471:478	the newly developed cellulose solution	441:478	the newly developed cellulose solution	441:478	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	3	6	theme	functional	1012:1021	arg1	materials					1049:1057	hydrogel-based functional composite green catalytic materials	997:1057	hydrogel-based functional composite green catalytic materials	997:1057	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	1	7	theme	magnetic	369:376	arg1	recyclability					378:390	magnetic recyclability	369:390	magnetic recyclability	369:390	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	0	8	theme	materials	221:229	arg1	topic					207:211	a hot research topic	192:211	a hot research topic of soft materials science and green catalysis	192:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	9	theme	solution	471:478	arg1	advantages					427:436	the advantages	423:436	the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2	423:512	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	1	10	theme	carboxylcellulose	277:293	arg1	hydrogel					295:302	a carboxylcellulose hydrogel	275:302	a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability	275:390	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	0	11	theme	preparation	116:126	arg1	degradation					81:91	Fenton-like degradation	69:91	Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	69:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	12	theme	soft	216:219	arg1	materials					221:229	soft materials science and green catalysis	216:257	soft materials science and green catalysis	216:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	12	theme	soft	216:219	arg1	science					231:237	science	231:237	science	231:237	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	12	theme	soft	216:219	arg1	catalysis					249:257	green catalysis	243:257	green catalysis	243:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	13	with	catalyst	343:350	arg1	recyclability					378:390	magnetic recyclability	369:390	magnetic recyclability	369:390	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	2	14	with	comparison	861:870	arg1	hydrogel					893:900	Fe3O4@cellulose hydrogel	877:900	Fe3O4@cellulose hydrogel (CH)	877:905	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	14	with	comparison	861:870	arg1	recyclability					922:934	excellent recyclability	912:934	excellent recyclability	912:934	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	14	with	comparison	861:870	arg1	CH					903:904	CH	903:904	CH	903:904	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	15	theme	hydrogen	778:785	arg1	peroxide					787:794	hydrogen peroxide	778:794	hydrogen peroxide	778:794	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	16	theme	cellulose	883:891	arg1	hydrogel					893:900	Fe3O4@cellulose hydrogel	877:900	Fe3O4@cellulose hydrogel (CH)	877:905	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	16	theme	cellulose	883:891	arg1	CH					903:904	CH	903:904	CH	903:904	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	0	17	theme	hydrogel	142:149	arg1	materials					151:159	functional hydrogel materials	131:159	functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	131:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	2	18	theme	efficient	662:670	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	18	theme	efficient	662:670	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	1	19	theme	tetramethyl	483:493	arg1	guanidine/DMSO/CO2					495:512	tetramethyl guanidine/DMSO/CO2	483:512	tetramethyl guanidine/DMSO/CO2	483:512	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	2	20	theme	@	615:615	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	20	theme	@	615:615	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	0	21	theme	functional	131:140	arg1	materials					151:159	functional hydrogel materials	131:159	functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	131:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	3	22	theme	composite	1023:1031	arg1	materials					1049:1057	hydrogel-based functional composite green catalytic materials	997:1057	hydrogel-based functional composite green catalytic materials	997:1057	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	0	23	theme	hydrogel	18:25	arg1	confined-Fe3O4					27:40	Carboxylcellulose hydrogel confined-Fe3O4	0:40	Carboxylcellulose hydrogel confined-Fe3O4	0:40	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	24	dep	Facile	109:114	arg1	B.					106:107	B.	106:107	B.	106:107	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	25	theme	Carboxylcellulose	0:16	arg1	confined-Fe3O4					27:40	Carboxylcellulose hydrogel confined-Fe3O4	0:40	Carboxylcellulose hydrogel confined-Fe3O4	0:40	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	3	26	theme	materials	1049:1057	arg1	preparation					982:992	the preparation	978:992	the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis	978:1110	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	0	27	theme	green	243:247	arg1	materials					221:229	soft materials science and green catalysis	216:257	soft materials science and green catalysis	216:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	27	theme	green	243:247	arg1	catalysis					249:257	green catalysis	243:257	green catalysis	243:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	28	theme	in	522:523	arg1	acylation					530:538	in situ acylation	522:538	in situ acylation using mixed cyclic anhydrides and ion exchange reaction	522:594	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	3	29	contain	has	1066:1068	arg2	applications					1080:1091	potential applications	1070:1091	potential applications	1070:1091	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	3	29	contain	has	1066:1068	arg1	materials					1049:1057	hydrogel-based functional composite green catalytic materials	997:1057	hydrogel-based functional composite green catalytic materials	997:1057	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	2	30	theme	excellent	912:920	arg1	recyclability					922:934	excellent recyclability	912:934	excellent recyclability	912:934	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	31	theme	achieved	601:608	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	31	theme	achieved	601:608	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	32	theme	Fe3O4	610:614	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	32	theme	Fe3O4	610:614	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	3	33	theme	green	1033:1037	arg1	materials					1049:1057	hydrogel-based functional composite green catalytic materials	997:1057	hydrogel-based functional composite green catalytic materials	997:1057	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	0	34	dep	materials	221:229	arg1	materials					221:229	soft materials science and green catalysis	216:257	soft materials science and green catalysis	216:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	34	dep	materials	221:229	arg1	science					231:237	science	231:237	science	231:237	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	34	dep	materials	221:229	arg1	catalysis					249:257	green catalysis	243:257	green catalysis	243:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	0	35	theme	materials	151:159	arg1	preparation					116:126	Rhodamine B. Facile preparation	96:126	Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	96:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	36	theme	composite	333:341	arg1	CHC					359:361	Fe3O4@CHC	353:361	Fe3O4@CHC	353:361	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	1	36	theme	composite	333:341	arg1	catalyst					343:350	composite catalyst	333:350	composite catalyst (Fe3O4@CHC) with magnetic recyclability	333:390	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	2	37	theme	peroxide	787:794	arg1	presence					766:773	the presence	762:773	the presence of hydrogen peroxide	762:794	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	38	theme	organic	729:735	arg1	RhB					754:756	RhB	754:756	RhB	754:756	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	38	theme	organic	729:735	arg1	B					751:751	the organic dye rhodamine B	725:751	the organic dye rhodamine B (RhB)	725:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	0	39	theme	environmental	165:177	arg1	catalysis					179:187	environmental catalysis	165:187	environmental catalysis is a hot research topic of soft materials science and green catalysis	165:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	2	40	theme	dye	737:739	arg1	RhB					754:756	RhB	754:756	RhB	754:756	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	40	theme	dye	737:739	arg1	B					751:751	the organic dye rhodamine B	725:751	the organic dye rhodamine B (RhB)	725:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	41	theme	B	751:751	arg1	decomposition					708:720	decomposition	708:720	decomposition of the organic dye rhodamine B (RhB)	708:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	42	theme	hydrogel	620:627	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	42	theme	hydrogel	620:627	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	3	43	theme	facile	958:963	arg1	strategy					965:972	a facile strategy	956:972	a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis	956:1110	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	2	44	theme	rhodamine	741:749	arg1	RhB					754:756	RhB	754:756	RhB	754:756	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	44	theme	rhodamine	741:749	arg1	B					751:751	the organic dye rhodamine B	725:751	the organic dye rhodamine B (RhB)	725:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	0	45	theme	Fenton-like	69:79	arg1	degradation					81:91	Fenton-like degradation	69:91	Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	69:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	46	theme	Fe3O4	353:357	arg1	CHC					359:361	Fe3O4@CHC	353:361	Fe3O4@CHC	353:361	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	1	46	theme	Fe3O4	353:357	arg1	catalyst					343:350	composite catalyst	333:350	composite catalyst (Fe3O4@CHC) with magnetic recyclability	333:390	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	2	47	theme	CHC	616:618	arg1	catalyst					629:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst	597:636	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	47	theme	CHC	616:618	arg1	catalyst					695:702	an more efficient and better Fenton-like catalyst	654:702	an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB)	654:757	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	1	48	theme	mixed	546:550	arg1	anhydrides					559:568	mixed cyclic anhydrides and ion exchange reaction	546:594	anhydrides	559:568	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	3	49	theme	potential	1070:1078	arg1	applications					1080:1091	potential applications	1070:1091	potential applications	1070:1091	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	1	50	theme	@	358:358	arg1	CHC					359:361	Fe3O4@CHC	353:361	Fe3O4@CHC	353:361	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	1	50	theme	@	358:358	arg1	catalyst					343:350	composite catalyst	333:350	composite catalyst (Fe3O4@CHC) with magnetic recyclability	333:390	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	0	51	theme	research	198:205	arg1	topic					207:211	a hot research topic	192:211	a hot research topic of soft materials science and green catalysis	192:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	52	theme	cyclic	552:557	arg1	anhydrides					559:568	mixed cyclic anhydrides and ion exchange reaction	546:594	anhydrides	559:568	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	2	53	from	catalyst	695:702	arg1	comparison					861:870	comparison	861:870	comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability	861:934	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	53	from	catalyst	695:702	arg1	presence					766:773	the presence	762:773	the presence of hydrogen peroxide	762:794	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	54	theme	@	882:882	arg1	hydrogel					893:900	Fe3O4@cellulose hydrogel	877:900	Fe3O4@cellulose hydrogel (CH)	877:905	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	54	theme	@	882:882	arg1	CH					903:904	CH	903:904	CH	903:904	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	0	55	theme	hot	194:196	arg1	topic					207:211	a hot research topic	192:211	a hot research topic of soft materials science and green catalysis	192:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	2	56	theme	Fe3O4	877:881	arg1	hydrogel					893:900	Fe3O4@cellulose hydrogel	877:900	Fe3O4@cellulose hydrogel (CH)	877:905	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	2	56	theme	Fe3O4	877:881	arg1	CH					903:904	CH	903:904	CH	903:904	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	0	57	theme	Rhodamine	96:104	arg1	preparation					116:126	Rhodamine B. Facile preparation	96:126	Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis	96:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	1	58	theme	developed	451:459	arg1	solution					471:478	the newly developed cellulose solution	441:478	the newly developed cellulose solution	441:478	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	3	59	theme	catalytic	1039:1047	arg1	materials					1049:1057	hydrogel-based functional composite green catalytic materials	997:1057	hydrogel-based functional composite green catalytic materials	997:1057	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	3	60	theme	hydrogel-based	997:1010	arg1	materials					1049:1057	hydrogel-based functional composite green catalytic materials	997:1057	hydrogel-based functional composite green catalytic materials	997:1057	This work provided a facile strategy for the preparation of hydrogel-based functional composite green catalytic materials, which has potential applications in green catalysis.					
33872611	0	61	dep	catalysis	179:187	arg1	topic					207:211	a hot research topic	192:211	a hot research topic of soft materials science and green catalysis	192:257	Carboxylcellulose hydrogel confined-Fe3O4 nanoparticles catalyst for Fenton-like degradation of Rhodamine B. Facile preparation of functional hydrogel materials for environmental catalysis is a hot research topic of soft materials science and green catalysis.					
33872611	2	62	theme	complete	809:816	arg1	decomposition					818:830	almost complete decomposition	802:830	almost complete decomposition occurring within 180 min	802:855	The achieved Fe3O4@CHC hydrogel catalyst was shown to be an more efficient and better Fenton-like catalyst for decomposition of the organic dye rhodamine B (RhB) in the presence of hydrogen peroxide, with almost complete decomposition occurring within 180 min, in comparison with Fe3O4@cellulose hydrogel (CH) with excellent recyclability.					
33872611	1	63	theme	ion	574:576	arg1	reaction					587:594	mixed cyclic anhydrides and ion exchange reaction	546:594	reaction	587:594	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	1	64	dep	in	522:523	arg1	situ					525:528	situ	525:528	situ	525:528	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33872611	1	65	from	advantages	427:436	arg1	guanidine/DMSO/CO2					495:512	tetramethyl guanidine/DMSO/CO2	483:512	tetramethyl guanidine/DMSO/CO2	483:512	In this study, a carboxylcellulose hydrogel confined Fe3O4 nanoparticles composite catalyst (Fe3O4@CHC) with magnetic recyclability has been synthesized by taking the advantages of the newly developed cellulose solution in tetramethyl guanidine/DMSO/CO2 through in situ acylation using mixed cyclic anhydrides and ion exchange reaction.					
33924078	7	0	theme	carp	1149:1152	arg1	isolates					1164:1171	carp and trout isolates	1149:1171	isolates	1164:1171	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	7	1	theme	new	1065:1067	arg1	immunotypes					1131:1141	the most commonly represented immunotypes	1101:1141	the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp	1101:1187	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	7	1	theme	new	1065:1067	arg1	one					1094:1096	one	1094:1096	one	1094:1096	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	7	1	theme	new	1065:1067	arg1	serogroup					1069:1077	the new serogroup PGO1	1061:1082	the new serogroup PGO1	1061:1082	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	3	2	theme	aeromonad	476:484	arg1	septicemia					486:495	aeromonad septicemia	476:495	aeromonad septicemia (MAI/MAS) on a Polish fish farm	476:527	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	3	2	theme	aeromonad	476:484	arg1	MAI/MAS					498:504	MAI/MAS	498:504	MAI/MAS	498:504	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	4	3	dep	1H	701:702	arg1	spectroscopy					716:727	NMR spectroscopy	712:727	NMR spectroscopy	712:727	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	9	4	theme	O-antigens	1415:1424	arg1	studies					1390:1396	studies	1390:1396	studies of the Aeromonas O-antigens	1390:1424	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	4	5	theme	structural	534:543	arg1	characterization					545:560	The structural characterization	530:560	The structural characterization	530:560	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	7	6	theme	immunotypes	1131:1141	arg1	immunotypes					1131:1141	the most commonly represented immunotypes	1101:1141	the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp	1101:1187	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	7	6	theme	immunotypes	1131:1141	arg1	one					1094:1096	one	1094:1096	one	1094:1096	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	7	6	theme	immunotypes	1131:1141	arg1	serogroup					1069:1077	the new serogroup PGO1	1061:1082	the new serogroup PGO1	1061:1082	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	3	7	from	septicemia	486:495	arg1	farm					524:527	a Polish fish farm	510:527	a Polish fish farm	510:527	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	9	8	theme	data	1471:1474	arg1	light					1446:1450	the light	1442:1450	the light of epidemiological data	1442:1474	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	9	9	theme	antigenic	1531:1539	arg1	variants					1541:1548	unknown antigenic variants	1523:1548	unknown antigenic variants	1523:1548	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	7	10	theme	represented	1119:1129	arg1	immunotypes					1131:1141	the most commonly represented immunotypes	1101:1141	the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp	1101:1187	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	3	11	theme	Polish	512:517	arg1	farm					524:527	a Polish fish farm	510:527	a Polish fish farm	510:527	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	5	12	theme	sugar	860:864	arg1	bacillosamine					904:916	bacillosamine	904:916	bacillosamine	904:916	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	5	12	theme	sugar	860:864	arg1	2,4-diamino-2,4,6-trideoxy-D-glucose					866:901	a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose	841:901	a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine)	841:917	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	2	13	theme	O-specific	278:287	arg1	O-PS					305:308	O-PS	305:308	O-PS	305:308	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	2	13	theme	O-specific	278:287	arg1	polysaccharide					289:302	the O-specific polysaccharide	274:302	especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv	263:333	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	2	13	theme	O-specific	278:287	arg1	LPS					258:260	LPS	258:260	LPS	258:260	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	3	14	theme	motile	442:447	arg1	aeromonad					449:457	motile aeromonad	442:457	motile aeromonad	442:457	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	3	15	theme	sobria	336:341	arg1	K133					350:353	sobria strain K133	336:353	sobria strain K133	336:353	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	3	16	theme	aeromonad	449:457	arg1	outbreak					430:437	an outbreak	427:437	an outbreak of motile aeromonad	427:457	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	9	17	from	light	1446:1450	arg1	relevant					1430:1437	relevant	1430:1437	relevant	1430:1437	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	1	18	theme	sobria	70:75	arg1	K133					84:87	sobria Strain K133	70:87	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms	70:195	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	5	19	theme	linear	790:795	arg1	tetrasaccharide					797:811	a linear tetrasaccharide	788:811	a linear tetrasaccharide repeating unit	788:826	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	1	20	theme	Polish	179:184	arg1	Farms					191:195	Polish Fish Farms	179:195	Polish Fish Farms	179:195	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	3	21	theme	strain	343:348	arg1	K133					350:353	sobria strain K133	336:353	sobria strain K133	336:353	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	9	22	from	relevant	1430:1437	arg1	light					1446:1450	the light	1442:1450	the light of epidemiological data	1442:1474	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	1	23	theme	Strain	77:82	arg1	K133					84:87	sobria Strain K133	70:87	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms	70:195	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	1	24	theme	Fish	186:189	arg1	Farms					191:195	Polish Fish Farms	179:195	Polish Fish Farms	179:195	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	9	25	theme	freshwater	1285:1294	arg1	species					1296:1302	freshwater species	1285:1302	freshwater species	1285:1302	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	7	26	theme	sobria	1031:1036	arg1	K133					1045:1048	sobria strain K133	1031:1048	sobria strain K133	1031:1048	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	9	27	theme	emergent	1491:1498	arg1	pathogens					1500:1508	emergent pathogens	1491:1508	emergent pathogens representing unknown antigenic variants and serotypes	1491:1562	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	9	28	theme	MAI/MAS	1246:1252	arg1	incidences					1254:1263	the MAI/MAS incidences	1242:1263	the MAI/MAS incidences	1242:1263	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	4	29	theme	LPS	626:628	arg1	degradation					607:617	mild acid degradation	597:617	mild acid degradation of the LPS	597:628	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	0	30	theme	Structural	0:9	arg1	Studies					11:17	Structural Studies	0:17	Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv.	0:68	Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv.					
33924078	7	31	theme	strain	1038:1043	arg1	K133					1045:1048	sobria strain K133	1031:1048	sobria strain K133	1031:1048	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	2	32	dep	Aeromonas	314:322	arg1	veronii					324:330	veronii	324:330	veronii	324:330	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	5	33	theme	tetrasaccharide	797:811	arg1	composition					773:783	a unique composition	764:783	a unique composition of a linear tetrasaccharide repeating unit	764:826	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	5	34	contain	has	760:762	arg2	composition					773:783	a unique composition	764:783	a unique composition of a linear tetrasaccharide repeating unit	764:826	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	5	34	contain	has	760:762	arg1	O-PS					755:758	the O-PS	751:758	the O-PS	751:758	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	3	35	theme	fish	519:522	arg1	farm					524:527	a Polish fish farm	510:527	a Polish fish farm	510:527	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	5	36	theme	serogroup	963:971	arg1	specificity					944:954	the specificity	940:954	the specificity of the serogroup	940:971	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	8	37	theme	Polish	1193:1198	arg1	aquacultures					1200:1211	Polish aquacultures	1193:1211	Polish aquacultures	1193:1211	in Polish aquacultures.					
33924078	4	38	theme	mass	678:681	arg1	spectrometry					683:694	MALDI-TOF mass spectrometry	668:694	MALDI-TOF mass spectrometry	668:694	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	4	39	theme	NMR	712:714	arg1	spectroscopy					716:727	NMR spectroscopy	712:727	NMR spectroscopy	712:727	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	1	40	theme	New	106:108	arg1	Serogroup					122:130	New Provisional Serogroup PGO1	106:135	New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms	106:195	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	9	41	theme	epidemiological	1455:1469	arg1	data					1471:1474	epidemiological data	1455:1474	epidemiological data	1455:1474	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	6	42	dep	A.	1016:1017	arg1	veronii					1019:1025	veronii	1019:1025	veronii	1019:1025	Western blotting and ELISA confirmed that A. veronii bv.					
33924078	9	43	from	increase	1230:1237	arg1	absence					1347:1353	the absence	1343:1353	the absence of an effective immunoprophylaxis	1343:1387	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	9	43	from	increase	1230:1237	arg1	incidences					1254:1263	the MAI/MAS incidences	1242:1263	the MAI/MAS incidences	1242:1263	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	4	44	theme	chemical	650:657	arg1	methods					659:665	chemical methods	650:665	chemical methods	650:665	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	5	45	theme	unique	766:771	arg1	composition					773:783	a unique composition	764:783	a unique composition of a linear tetrasaccharide repeating unit	764:826	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	2	46	theme	immunochemical	232:245	arg1	studies					247:253	immunochemical studies	232:253	immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv	232:333	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	6	47	theme	A.	1016:1017	arg1	bv					1027:1028	that A. veronii bv	1011:1028	that A. veronii bv	1011:1028	Western blotting and ELISA confirmed that A. veronii bv.					
33924078	4	48	theme	acid	602:605	arg1	degradation					607:617	mild acid degradation	597:617	mild acid degradation of the LPS	597:628	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	0	49	theme	Lipopolysaccharide	26:43	arg1	Studies					11:17	Structural Studies	0:17	Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv.	0:68	Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv.					
33924078	3	50	theme	carp	394:397	arg1	kidney					384:389	the kidney	380:389	the kidney of carp (Cyprinus carpio L.)	380:418	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	3	51	dep	Cyprinus	400:407	arg1	L.					416:417	Cyprinus carpio L.	400:417	Cyprinus carpio L.	400:417	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	4	52	theme	mild	597:600	arg1	degradation					607:617	mild acid degradation	597:617	mild acid degradation of the LPS	597:628	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	0	53	theme	veronii	58:64	arg1	Lipopolysaccharide					26:43	the Lipopolysaccharide	22:43	the Lipopolysaccharide of Aeromonas veronii bv	22:67	Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv.					
33924078	1	54	theme	Provisional	110:120	arg1	Serogroup					122:130	New Provisional Serogroup PGO1	106:135	New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms	106:195	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	0	55	theme	Aeromonas	48:56	arg1	veronii					58:64	Aeromonas veronii bv	48:67	Aeromonas veronii bv	48:67	Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv.					
33924078	7	56	theme	Aeromonas	1176:1184	arg1	sp					1186:1187	Aeromonas sp	1176:1187	Aeromonas sp	1176:1187	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	3	57	attach	isolated	366:373	arg1	kidney					384:389	the kidney	380:389	the kidney of carp (Cyprinus carpio L.)	380:418	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	3	57	attach	isolated	366:373	arg2	K133					350:353	sobria strain K133	336:353	sobria strain K133	336:353	sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.					
33924078	2	58	theme	bv	332:333	arg1	O-PS					305:308	O-PS	305:308	O-PS	305:308	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	2	58	theme	bv	332:333	arg1	polysaccharide					289:302	the O-specific polysaccharide	274:302	especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv	263:333	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	2	58	theme	bv	332:333	arg1	LPS					258:260	LPS	258:260	LPS	258:260	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	2	59	theme	present	205:211	arg1	work					213:216	the present work	201:216	the present work	201:216	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	6	60	theme	Western	974:980	arg1	blotting					982:989	Western blotting	974:989	Western blotting	974:989	Western blotting and ELISA confirmed that A. veronii bv.					
33924078	4	61	theme	MALDI-TOF	668:676	arg1	spectrometry					683:694	MALDI-TOF mass spectrometry	668:694	MALDI-TOF mass spectrometry	668:694	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	9	62	theme	unknown	1523:1529	arg1	variants					1541:1548	unknown antigenic variants	1523:1548	unknown antigenic variants	1523:1548	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	9	63	theme	economic	1315:1322	arg1	importance					1324:1333	economic importance	1315:1333	economic importance	1315:1333	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	5	64	theme	occurring	850:858	arg1	bacillosamine					904:916	bacillosamine	904:916	bacillosamine	904:916	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	5	64	theme	occurring	850:858	arg1	2,4-diamino-2,4,6-trideoxy-D-glucose					866:901	a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose	841:901	a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine)	841:917	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	2	65	theme	LPS	258:260	arg1	studies					247:253	immunochemical studies	232:253	immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv	232:333	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	5	66	contain	contains	832:839	arg2	2,4-diamino-2,4,6-trideoxy-D-glucose					866:901	a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose	841:901	a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine)	841:917	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	5	66	contain	contains	832:839	arg2	bacillosamine					904:916	bacillosamine	904:916	bacillosamine	904:916	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	5	66	contain	contains	832:839	arg1	O-PS					755:758	the O-PS	751:758	the O-PS	751:758	It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup.					
33924078	2	67	theme	Aeromonas	314:322	arg1	bv					332:333	Aeromonas veronii bv	314:333	Aeromonas veronii bv	314:333	In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv.					
33924078	4	68	theme	O-PS	569:572	arg1	characterization					545:560	The structural characterization	530:560	The structural characterization	530:560	The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy.					
33924078	7	69	theme	trout	1158:1162	arg1	isolates					1164:1171	carp and trout isolates	1149:1171	isolates	1164:1171	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	9	70	theme	Aeromonas	1405:1413	arg1	O-antigens					1415:1424	the Aeromonas O-antigens	1401:1424	the Aeromonas O-antigens	1401:1424	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	9	71	from	impact	1275:1280	arg1	species					1296:1302	freshwater species	1285:1302	freshwater species	1285:1302	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	7	72	theme	sp	1186:1187	arg1	isolates					1164:1171	carp and trout isolates	1149:1171	isolates	1164:1171	sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp.					
33924078	9	73	theme	effective	1361:1369	arg1	immunoprophylaxis					1371:1387	an effective immunoprophylaxis	1358:1387	an effective immunoprophylaxis	1358:1387	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	9	74	theme	immunoprophylaxis	1371:1387	arg1	absence					1347:1353	the absence	1343:1353	the absence of an effective immunoprophylaxis	1343:1387	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
33924078	1	75	theme	Mesophilic	154:163	arg1	Aeromonads					165:174	Mesophilic Aeromonads	154:174	Mesophilic Aeromonads	154:174	sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.					
33924078	9	76	with	increase	1230:1237	arg1	importance					1324:1333	economic importance	1315:1333	economic importance	1315:1333	Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.					
34862923	10	0	from	activity	1249:1256	arg1	stem					1261:1264	stem	1261:1264	stem	1261:1264	In SbSAI-2-1 overexpression lines, the invertase activity in stem was increased by more than 20 times (P < 0.01).					
34862923	3	1	from	function	431:438	arg1	sorghum					480:486	sorghum	480:486	sorghum	480:486	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	6	2	theme	overexpression	802:815	arg1	lines					817:821	SbSAI-2-1 overexpression lines	792:821	SbSAI-2-1 overexpression lines	792:821	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	10	3	theme	SbSAI-2-1	1203:1211	arg1	lines					1228:1232	SbSAI-2-1 overexpression lines	1203:1232	SbSAI-2-1 overexpression lines	1203:1232	In SbSAI-2-1 overexpression lines, the invertase activity in stem was increased by more than 20 times (P < 0.01).					
34862923	3	4	theme	transferase	460:470	arg1	gene					472:475	the soluble acid transferase gene	443:475	the soluble acid transferase gene in sorghum	443:486	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	6	5	theme	mutant	775:780	arg1	lines					782:786	Osvin2 mutant lines	768:786	Osvin2 mutant lines	768:786	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	8	6	theme	invertase	896:904	arg1	functionality					906:918	the invertase functionality	892:918	the invertase functionality	892:918	Nipponbare were produced to clarify the invertase functionality.					
34862923	1	7	from	content	245:251	arg1	juice					270:274	stem juice	265:274	stem juice	265:274	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	3	8	from	structure	417:425	arg1	sorghum					480:486	sorghum	480:486	sorghum	480:486	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	1	9	from	composition	229:239	arg1	juice					270:274	stem juice	265:274	stem juice	265:274	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	6	10	dep	sativa	832:837	arg1	japonica					842:849	Oryza sativa L. japonica	826:849	Oryza sativa L. japonica	826:849	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	11	11	dep	respectively	1406:1417	arg1	P					1420:1420	P < 0.01	1420:1427	P < 0.01	1420:1427	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	4	12	theme	sorghum	556:562	arg1	BTx623					572:577	the sorghum variety BTx623	552:577	the sorghum variety BTx623	552:577	In this study, SbSAI-2 was cloned from the sorghum variety BTx623, and two transcripts were found through sequence analysis, with only one transcript translated into an active protein.					
34862923	4	13	theme	sequence	619:626	arg1	analysis					628:635	sequence analysis	619:635	sequence analysis	619:635	In this study, SbSAI-2 was cloned from the sorghum variety BTx623, and two transcripts were found through sequence analysis, with only one transcript translated into an active protein.					
34862923	1	14	theme	sorghum	91:97	arg1	SbSAI-2					127:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	11	15	theme	fructose	1342:1349	arg1	contents					1318:1325	The contents	1314:1325	The contents of glucose and fructose in stem tissues	1314:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	9	16	theme	invertase	931:939	arg1	activity					941:948	the invertase activity	927:948	the invertase activity in the stem of the Osvin2 mutant line	927:986	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	11	17	theme	detection	1509:1517	arg1	P					1526:1526	P < 0.01	1526:1533	P < 0.01	1526:1533	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	11	17	theme	detection	1509:1517	arg1	limit					1519:1523	the detection limit	1505:1523	the detection limit (P < 0.01)	1505:1534	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	1	18	theme	acid	107:110	arg1	SbSAI-2					127:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	1	19	theme	soluble	99:105	arg1	SbSAI-2					127:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	11	20	from	glucose	1330:1336	arg1	tissues					1359:1365	stem tissues	1354:1365	stem tissues	1354:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	11	21	from	fructose	1342:1349	arg1	tissues					1359:1365	stem tissues	1354:1365	stem tissues	1354:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	0	22	from	Cloning	0:6	arg1	sorghum					78:84	sorghum	78:84	sorghum	78:84	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	13	23	theme	gene	1708:1711	arg1	verification					1722:1733	the gene function verification	1704:1733	the gene function verification	1704:1733	The result provided further the gene function verification and laid a foundation for the development of molecular markers.					
34862923	4	24	theme	active	682:687	arg1	protein					689:695	an active protein	679:695	an active protein	679:695	In this study, SbSAI-2 was cloned from the sorghum variety BTx623, and two transcripts were found through sequence analysis, with only one transcript translated into an active protein.					
34862923	9	25	theme	mutant	976:981	arg1	line					983:986	the Osvin2 mutant line	965:986	the Osvin2 mutant line	965:986	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	9	26	theme	sucrose	1160:1166	arg1	content					1149:1155	the content	1145:1155	the content of sucrose	1145:1166	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	11	27	from	contents	1318:1325	arg1	tissues					1359:1365	stem tissues	1354:1365	stem tissues	1354:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	9	28	from	activity	941:948	arg1	stem					957:960	the stem	953:960	the stem of the Osvin2 mutant line	953:986	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	2	29	theme	processes	333:341	arg1	one					297:299	one	297:299	one	297:299	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	2	29	theme	processes	333:341	arg1	processes					333:341	the most important metabolic processes	304:341	the most important metabolic processes	304:341	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	5	30	theme	%	709:709	arg1	homology					711:718	72% homology	707:718	72% homology between SbSAI-2 and OsVIN2	707:745	There is 72% homology between SbSAI-2 and OsVIN2.					
34862923	9	31	theme	fructose	1064:1071	arg1	glucose					1077:1083	glucose	1077:1083	glucose in stem tissue	1077:1098	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	9	31	theme	fructose	1064:1071	arg1	contents					1052:1059	the contents	1048:1059	the contents of fructose	1048:1071	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	2	32	theme	soluble	363:369	arg1	invertase					376:384	soluble acid invertase	363:384	soluble acid invertase	363:384	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	0	33	theme	functional	12:21	arg1	analysis					23:30	functional analysis	12:30	functional analysis	12:30	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	12	34	theme	sucrose	1598:1604	arg1	metabolism					1606:1615	sucrose metabolism	1598:1615	sucrose metabolism	1598:1615	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	6	35	theme	Osvin2	768:773	arg1	lines					782:786	Osvin2 mutant lines	768:786	Osvin2 mutant lines	768:786	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	8	36	dep	produced	872:879	arg1	Nipponbare					856:865	Nipponbare	856:865	Nipponbare	856:865	Nipponbare were produced to clarify the invertase functionality.					
34862923	2	37	from	processes	333:341	arg1	plants					346:351	plants	346:351	plants	346:351	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	11	38	theme	sucrose	1452:1458	arg1	content					1441:1447	the content	1437:1447	the content of sucrose	1437:1458	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	0	39	theme	acid	43:46	arg1	SbSAI-2					66:72	SbSAI-2	66:72	SbSAI-2	66:72	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	0	39	theme	acid	43:46	arg1	gene					60:63	soluble acid invertase 2 gene	35:63	soluble acid invertase 2 gene (SbSAI-2)	35:73	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	12	40	theme	key	1576:1578	arg1	SbSAI-2					1563:1569	SbSAI-2	1563:1569	SbSAI-2	1563:1569	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	12	40	theme	key	1576:1578	arg1	enzyme					1580:1585	a key enzyme	1574:1585	a key enzyme related to sucrose metabolism	1574:1615	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	13	41	theme	function	1713:1720	arg1	verification					1722:1733	the gene function verification	1704:1733	the gene function verification	1704:1733	The result provided further the gene function verification and laid a foundation for the development of molecular markers.					
34862923	12	42	theme	related	1587:1593	arg1	SbSAI-2					1563:1569	SbSAI-2	1563:1569	SbSAI-2	1563:1569	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	12	42	theme	related	1587:1593	arg1	enzyme					1580:1585	a key enzyme	1574:1585	a key enzyme related to sucrose metabolism	1574:1615	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	1	43	theme	gene	122:125	arg1	SbSAI-2					127:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	9	44	dep	change	1108:1113	arg1	P					1130:1130	P > 0.05	1130:1137	P > 0.05	1130:1137	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	2	45	theme	important	313:321	arg1	processes					333:341	the most important metabolic processes	304:341	the most important metabolic processes	304:341	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	6	46	theme	Oryza	826:830	arg1	sativa					832:837	Oryza sativa L. japonica	826:849	Oryza sativa L. japonica	826:849	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	6	47	theme	lines	817:821	arg1	construction					752:763	The construction	748:763	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.	748:850	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	10	48	theme	overexpression	1213:1226	arg1	lines					1228:1232	SbSAI-2-1 overexpression lines	1203:1232	SbSAI-2-1 overexpression lines	1203:1232	In SbSAI-2-1 overexpression lines, the invertase activity in stem was increased by more than 20 times (P < 0.01).					
34862923	3	49	theme	gene	472:475	arg1	structure					417:425	structure	417:425	structure	417:425	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	3	49	theme	gene	472:475	arg1	function					431:438	function	431:438	function	431:438	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	6	50	theme	SbSAI-2-1	792:800	arg1	lines					817:821	SbSAI-2-1 overexpression lines	792:821	SbSAI-2-1 overexpression lines	792:821	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	11	51	theme	glucose	1330:1336	arg1	contents					1318:1325	The contents	1314:1325	The contents of glucose and fructose in stem tissues	1314:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	3	52	theme	acid	455:458	arg1	gene					472:475	the soluble acid transferase gene	443:475	the soluble acid transferase gene in sorghum	443:486	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	6	53	theme	lines	782:786	arg1	construction					752:763	The construction	748:763	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.	748:850	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	4	54	theme	variety	564:570	arg1	BTx623					572:577	the sorghum variety BTx623	552:577	the sorghum variety BTx623	552:577	In this study, SbSAI-2 was cloned from the sorghum variety BTx623, and two transcripts were found through sequence analysis, with only one transcript translated into an active protein.					
34862923	6	55	from	construction	752:763	arg1	sativa					832:837	Oryza sativa L. japonica	826:849	Oryza sativa L. japonica	826:849	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	10	56	theme	invertase	1239:1247	arg1	activity					1249:1256	the invertase activity	1235:1256	the invertase activity in stem	1235:1264	In SbSAI-2-1 overexpression lines, the invertase activity in stem was increased by more than 20 times (P < 0.01).					
34862923	12	57	dep	composition	1633:1643	arg1	the					1629:1631	the	1629:1631	the	1629:1631	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	9	58	theme	stem	1088:1091	arg1	tissue					1093:1098	stem tissue	1088:1098	stem tissue	1088:1098	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	1	59	theme	Pichia	175:180	arg1	pastoris					182:189	Pichia pastoris	175:189	Pichia pastoris	175:189	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	6	60	theme	L.	839:840	arg1	sativa					832:837	Oryza sativa L. japonica	826:849	Oryza sativa L. japonica	826:849	The construction of Osvin2 mutant lines and SbSAI-2-1 overexpression lines in Oryza sativa L. japonica.					
34862923	3	61	from	sorghum	480:486	arg1	structure					417:425	structure	417:425	structure	417:425	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	3	61	from	sorghum	480:486	arg1	function					431:438	function	431:438	function	431:438	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	11	62	theme	stem	1354:1357	arg1	tissues					1359:1365	stem tissues	1354:1365	stem tissues	1354:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	11	63	from	tissues	1359:1365	arg1	contents					1318:1325	The contents	1314:1325	The contents of glucose and fructose in stem tissues	1314:1365	The contents of glucose and fructose in stem tissues were increased by two and three times, respectively (P < 0.01), while the content of sucrose was significantly decreased, which was below the detection limit (P < 0.01).					
34862923	9	64	from	glucose	1077:1083	arg1	tissue					1093:1098	stem tissue	1088:1098	stem tissue	1088:1098	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	0	65	theme	gene	60:63	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	0	65	theme	gene	60:63	arg1	analysis					23:30	functional analysis	12:30	functional analysis	12:30	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	1	66	theme	invertase	112:120	arg1	SbSAI-2					127:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2	87:133	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	0	67	from	analysis	23:30	arg1	sorghum					78:84	sorghum	78:84	sorghum	78:84	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	12	68	theme	sugar	1660:1664	arg1	content					1649:1655	content	1649:1655	content	1649:1655	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	12	68	theme	sugar	1660:1664	arg1	composition					1633:1643	composition	1633:1643	composition	1633:1643	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	12	69	from	content	1649:1655	arg1	stems					1669:1673	stems	1669:1673	stems	1669:1673	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	9	70	from	contents	1052:1059	arg1	tissue					1093:1098	stem tissue	1088:1098	stem tissue	1088:1098	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	3	71	from	gene	472:475	arg1	sorghum					480:486	sorghum	480:486	sorghum	480:486	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	12	72	from	composition	1633:1643	arg1	stems					1669:1673	stems	1669:1673	stems	1669:1673	This study indicated that SbSAI-2 is a key enzyme related to sucrose metabolism and affects the composition and content of sugar in stems.					
34862923	9	73	theme	Osvin2	969:974	arg1	line					983:986	the Osvin2 mutant line	965:986	the Osvin2 mutant line	965:986	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	13	74	theme	molecular	1780:1788	arg1	markers					1790:1796	molecular markers	1780:1796	molecular markers	1780:1796	The result provided further the gene function verification and laid a foundation for the development of molecular markers.					
34862923	3	75	dep	structure	417:425	arg1	the					413:415	the	413:415	the	413:415	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
34862923	9	76	theme	line	983:986	arg1	stem					957:960	the stem	953:960	the stem of the Osvin2 mutant line	953:986	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	5	77	theme	72	707:708	arg1	%					709:709	%	709:709	%	709:709	There is 72% homology between SbSAI-2 and OsVIN2.					
34862923	2	78	theme	metabolic	323:331	arg1	processes					333:341	the most important metabolic processes	304:341	the most important metabolic processes	304:341	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	2	79	theme	acid	371:374	arg1	invertase					376:384	soluble acid invertase	363:384	soluble acid invertase	363:384	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	10	80	theme	P	1303:1303	arg1	times					1296:1300	more than 20 times	1283:1300	more than 20 times (P < 0.01)	1283:1311	In SbSAI-2-1 overexpression lines, the invertase activity in stem was increased by more than 20 times (P < 0.01).					
34862923	10	80	theme	P	1303:1303	arg1	< 0.01					1305:1310	P < 0.01	1303:1310	P < 0.01	1303:1310	In SbSAI-2-1 overexpression lines, the invertase activity in stem was increased by more than 20 times (P < 0.01).					
34862923	0	81	theme	soluble	35:41	arg1	SbSAI-2					66:72	SbSAI-2	66:72	SbSAI-2	66:72	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	0	81	theme	soluble	35:41	arg1	gene					60:63	soluble acid invertase 2 gene	35:63	soluble acid invertase 2 gene (SbSAI-2)	35:73	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	1	82	theme	sugar	256:260	arg1	content					245:251	content	245:251	content	245:251	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	1	82	theme	sugar	256:260	arg1	composition					229:239	composition	229:239	composition	229:239	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	9	83	theme	significant	1009:1019	arg1	P					1033:1033	P > 0.05	1033:1040	P > 0.05	1033:1040	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	9	83	theme	significant	1009:1019	arg1	difference					1021:1030	no significant difference	1006:1030	no significant difference (P > 0.05)	1006:1041	While the invertase activity in the stem of the Osvin2 mutant line was reduced, with no significant difference (P > 0.05), and the contents of fructose and glucose in stem tissue did not change significantly (P > 0.05), and the content of sucrose increased by 38.89% (P < 0.01).					
34862923	0	84	theme	invertase	48:56	arg1	SbSAI-2					66:72	SbSAI-2	66:72	SbSAI-2	66:72	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	0	84	theme	invertase	48:56	arg1	gene					60:63	soluble acid invertase 2 gene	35:63	soluble acid invertase 2 gene (SbSAI-2)	35:73	Cloning and functional analysis of soluble acid invertase 2 gene (SbSAI-2) in sorghum.					
34862923	1	85	theme	stem	265:268	arg1	juice					270:274	stem juice	265:274	stem juice	265:274	The sorghum soluble acid invertase gene SbSAI-2 was cloned and the function verified in Pichia pastoris and rice, showing the SbSAI-2 affects composition and content of sugar in stem juice.					
34862923	13	86	theme	markers	1790:1796	arg1	development					1765:1775	the development	1761:1775	the development of molecular markers	1761:1796	The result provided further the gene function verification and laid a foundation for the development of molecular markers.					
34862923	2	87	theme	key	394:396	arg1	role					398:401	a key role	392:401	a key role	392:401	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	2	88	theme	Sugar	277:281	arg1	metabolism					283:292	Sugar metabolism	277:292	Sugar metabolism	277:292	Sugar metabolism is one of the most important metabolic processes in plants, in which soluble acid invertase plays a key role.					
34862923	3	89	theme	soluble	447:453	arg1	gene					472:475	the soluble acid transferase gene	443:475	the soluble acid transferase gene in sorghum	443:486	However, the structure and function of the soluble acid transferase gene in sorghum are still fully unclear.					
33182083	0	0	theme	Chromium	90:97	arg1	sequestration					73:85	the sequestration	69:85	the sequestration of Chromium(VI) and fluoride from water	69:125	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	1	1	theme	environmental	333:345	arg1	anxiety					347:353	environmental anxiety	333:353	environmental anxiety to the community	333:370	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	1	1	theme	environmental	333:345	arg1	problem					292:298	the frequent problem	279:298	the frequent problem of inorganic pollution	279:321	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	3	2	theme	low-cost	481:488	arg1	procedure					500:508	a low-cost synthesis procedure	479:508	a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water	479:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	1	3	theme	recent	254:259	arg1	decades					261:267	recent decades	254:267	recent decades	254:267	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	3	4	from	water	678:682	arg1	removal					644:650	the adsorptive removal	629:650	the adsorptive removal of toxic Cr6+ and F- from water	629:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	2	5	from	industries	442:451	arg1	Cr6+					386:389	Cr6+	386:389	Cr6+	386:389	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	5	from	industries	442:451	arg1	pollutants					421:430	priority poisonous pollutants	402:430	priority poisonous pollutants from many industries	402:451	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	5	from	industries	442:451	arg1	F-					395:396	F-	395:396	F-	395:396	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	6	theme	many	437:440	arg1	industries					442:451	many industries	437:451	many industries	437:451	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	5	7	theme	several	870:876	arg1	techniques					895:904	several physico-chemical techniques	870:904	several physico-chemical techniques to access their properties	870:931	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	1	8	theme	industrialization	147:163	arg1	development					132:142	The development	128:142	The development of industrialization and agricultural activities	128:191	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	5	9	theme	calorimetry	1181:1191	arg1	analysis					1199:1206	X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis	936:1206	X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis	936:1206	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	10	theme	electron	970:977	arg1	microscopy					979:988	scanning electron microscopy	961:988	scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA)	961:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	11	theme	diffraction	942:952	arg1	analysis					1199:1206	X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis	936:1206	X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis	936:1206	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	7	12	theme	maximum	1394:1400	arg1	138.89					1439:1444	138.89	1439:1444	138.89	1439:1444	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	7	12	theme	maximum	1394:1400	arg1	capacity					1413:1420	The maximum adsorption capacity	1390:1420	The maximum adsorption capacity for CS-Zr-GEL	1390:1434	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	4	13	theme	batch	743:747	arg1	mode					749:752	batch mode	743:752	batch mode	743:752	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	14	theme	adsorption	689:698	arg1	function					759:766	a function	757:766	a function of contact time, solution pH and co-existing ions	757:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	14	theme	adsorption	689:698	arg1	mechanism					700:708	The adsorption mechanism	685:708	The adsorption mechanism of toxic Cr6+ and F-	685:729	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	15	theme	ions	813:816	arg1	function					759:766	a function	757:766	a function of contact time, solution pH and co-existing ions	757:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	15	theme	ions	813:816	arg1	mechanism					700:708	The adsorption mechanism	685:708	The adsorption mechanism of toxic Cr6+ and F-	685:729	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	1	16	theme	various	206:212	arg1	impacts					223:229	various negative impacts	206:229	various negative impacts to living organisms	206:249	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	5	17	theme	obtained	823:830	arg1	materials					832:840	The obtained materials	819:840	The obtained materials	819:840	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	18	theme	physico-chemical	878:893	arg1	techniques					895:904	several physico-chemical techniques	870:904	several physico-chemical techniques to access their properties	870:931	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	4	19	theme	co-existing	801:811	arg1	ions					813:816	co-existing ions	801:816	co-existing ions	801:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	1	20	theme	living	234:239	arg1	organisms					241:249	living organisms	234:249	living organisms	234:249	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	1	21	theme	agricultural	169:180	arg1	activities					182:191	agricultural activities	169:191	agricultural activities	169:191	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	6	22	dep	solution	1271:1278	arg1	fitted					1319:1324	fitted	1319:1324	were fitted using pseudo-second-order kinetic and Langmuir isotherm models	1314:1387	Additionally, the fabricated adsorbent is highly dependent on solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models.					
33182083	6	22	dep	solution	1271:1278	arg1	pH.					1280:1282	solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models	1271:1387	solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models	1271:1387	Additionally, the fabricated adsorbent is highly dependent on solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models.					
33182083	0	23	dep	Chromium	90:97	arg1	VI					99:100	VI	99:100	VI	99:100	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	8	24	theme	practical	1606:1614	arg1	influence					1616:1624	a practical influence	1604:1624	a practical influence	1604:1624	These findings demonstrate that the CS-Zr-GEL adsorbent represents a promising candidate that would have a practical influence on water/wastewater treatments.					
33182083	1	25	theme	activities	182:191	arg1	development					132:142	The development	128:142	The development of industrialization and agricultural activities	128:191	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	0	26	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of Zr4+	0:23	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	5	27	theme	scanning	1172:1179	arg1	calorimetry					1181:1191	differential scanning calorimetry	1159:1191	differential scanning calorimetry	1159:1191	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	8	28	contain	have	1599:1602	arg1	candidate					1578:1586	a promising candidate	1566:1586	a promising candidate that would have a practical influence on water/wastewater treatments	1566:1655	These findings demonstrate that the CS-Zr-GEL adsorbent represents a promising candidate that would have a practical influence on water/wastewater treatments.					
33182083	8	28	contain	have	1599:1602	arg2	influence					1616:1624	a practical influence	1604:1624	a practical influence	1604:1624	These findings demonstrate that the CS-Zr-GEL adsorbent represents a promising candidate that would have a practical influence on water/wastewater treatments.					
33182083	4	29	theme	F-	728:729	arg1	function					759:766	a function	757:766	a function of contact time, solution pH and co-existing ions	757:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	29	theme	F-	728:729	arg1	mechanism					700:708	The adsorption mechanism	685:708	The adsorption mechanism of toxic Cr6+ and F-	685:729	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	5	30	theme	infrared	1061:1068	arg1	FTIR					1084:1087	FTIR	1084:1087	FTIR	1084:1087	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	30	theme	infrared	1061:1068	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	30	theme	infrared	1061:1068	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	0	31	theme	Zr4+	20:23	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of Zr4+	0:23	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	5	32	theme	microscopy	979:988	arg1	analysis					1199:1206	X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis	936:1206	X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis	936:1206	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	3	33	theme	chitosan-gelatin	557:572	arg1	composite					574:582	chitosan-gelatin composite	557:582	chitosan-gelatin composite (CS-Zr-GEL)	557:594	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	3	33	theme	chitosan-gelatin	557:572	arg1	CS-Zr-GEL					585:593	CS-Zr-GEL	585:593	CS-Zr-GEL	585:593	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	5	34	theme	Energy	995:1000	arg1	BET					1115:1117	BET	1115:1117	BET	1115:1117	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	34	theme	Energy	995:1000	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	34	theme	Energy	995:1000	arg1	SEM-EDX					1033:1039	SEM-EDX	1033:1039	SEM-EDX	1033:1039	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	34	theme	Energy	995:1000	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	34	theme	Energy	995:1000	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	2	35	theme	poisonous	411:419	arg1	Cr6+					386:389	Cr6+	386:389	Cr6+	386:389	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	35	theme	poisonous	411:419	arg1	pollutants					421:430	priority poisonous pollutants	402:430	priority poisonous pollutants from many industries	402:451	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	35	theme	poisonous	411:419	arg1	F-					395:396	F-	395:396	F-	395:396	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	7	36	theme	adsorption	1402:1411	arg1	138.89					1439:1444	138.89	1439:1444	138.89	1439:1444	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	7	36	theme	adsorption	1402:1411	arg1	capacity					1413:1420	The maximum adsorption capacity	1390:1420	The maximum adsorption capacity for CS-Zr-GEL	1390:1434	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	1	37	theme	frequent	283:290	arg1	anxiety					347:353	environmental anxiety	333:353	environmental anxiety to the community	333:370	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	1	37	theme	frequent	283:290	arg1	problem					292:298	the frequent problem	279:298	the frequent problem of inorganic pollution	279:321	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	0	38	theme	fluoride	107:114	arg1	sequestration					73:85	the sequestration	69:85	the sequestration of Chromium(VI) and fluoride from water	69:125	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	3	39	theme	toxic	655:659	arg1	Cr6+					661:664	toxic Cr6+	655:664	toxic Cr6+	655:664	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	3	40	theme	synthesis	490:498	arg1	procedure					500:508	a low-cost synthesis procedure	479:508	a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water	479:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	2	41	theme	priority	402:409	arg1	Cr6+					386:389	Cr6+	386:389	Cr6+	386:389	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	41	theme	priority	402:409	arg1	pollutants					421:430	priority poisonous pollutants	402:430	priority poisonous pollutants from many industries	402:451	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	2	41	theme	priority	402:409	arg1	F-					395:396	F-	395:396	F-	395:396	Among these, Cr6+ and F- are priority poisonous pollutants from many industries.					
33182083	0	42	theme	chitosan/gelatin	38:53	arg1	composite					55:63	chitosan/gelatin composite	38:63	chitosan/gelatin composite	38:63	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	5	43	theme	differential	1159:1170	arg1	calorimetry					1181:1191	differential scanning calorimetry	1159:1191	differential scanning calorimetry	1159:1191	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	6	44	theme	isotherm	1300:1307	arg1	data					1309:1312	The kinetic and isotherm data	1284:1312	data	1309:1312	Additionally, the fabricated adsorbent is highly dependent on solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models.					
33182083	3	45	theme	Cr6+	661:664	arg1	removal					644:650	the adsorptive removal	629:650	the adsorptive removal of toxic Cr6+ and F- from water	629:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	4	46	theme	solution	785:792	arg1	pH					794:795	solution pH	785:795	solution pH	785:795	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	1	47	theme	negative	214:221	arg1	impacts					223:229	various negative impacts	206:229	various negative impacts to living organisms	206:249	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	5	48	theme	thermogravimetric	1121:1137	arg1	TGA					1149:1151	TGA	1149:1151	TGA	1149:1151	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	48	theme	thermogravimetric	1121:1137	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	48	theme	thermogravimetric	1121:1137	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	49	theme	Dispersive	1002:1011	arg1	BET					1115:1117	BET	1115:1117	BET	1115:1117	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	49	theme	Dispersive	1002:1011	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	49	theme	Dispersive	1002:1011	arg1	SEM-EDX					1033:1039	SEM-EDX	1033:1039	SEM-EDX	1033:1039	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	49	theme	Dispersive	1002:1011	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	49	theme	Dispersive	1002:1011	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	50	theme	Fourier-transform	1043:1059	arg1	FTIR					1084:1087	FTIR	1084:1087	FTIR	1084:1087	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	50	theme	Fourier-transform	1043:1059	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	50	theme	Fourier-transform	1043:1059	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	1	51	theme	inorganic	303:311	arg1	pollution					313:321	inorganic pollution	303:321	inorganic pollution	303:321	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	6	52	theme	kinetic	1288:1294	arg1	data					1309:1312	The kinetic and isotherm data	1284:1312	data	1309:1312	Additionally, the fabricated adsorbent is highly dependent on solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models.					
33182083	5	53	theme	X-ray	1013:1017	arg1	BET					1115:1117	BET	1115:1117	BET	1115:1117	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	53	theme	X-ray	1013:1017	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	53	theme	X-ray	1013:1017	arg1	SEM-EDX					1033:1039	SEM-EDX	1033:1039	SEM-EDX	1033:1039	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	53	theme	X-ray	1013:1017	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	53	theme	X-ray	1013:1017	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	7	54	from	323K	1464:1467	arg1	12.13 mg/g					1450:1459	12.13 mg/g	1450:1459	12.13 mg/g	1450:1459	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	7	54	from	323K	1464:1467	arg1	138.89					1439:1444	138.89	1439:1444	138.89	1439:1444	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	7	54	from	323K	1464:1467	arg1	capacity					1413:1420	The maximum adsorption capacity	1390:1420	The maximum adsorption capacity for CS-Zr-GEL	1390:1434	The maximum adsorption capacity for CS-Zr-GEL is 138.89 and 12.13 mg/g at 323K for Cr6+ and F- respectively.					
33182083	4	55	theme	toxic	713:717	arg1	Cr6+					719:722	toxic Cr6+	713:722	toxic Cr6+	713:722	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	1	56	theme	pollution	313:321	arg1	anxiety					347:353	environmental anxiety	333:353	environmental anxiety to the community	333:370	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	1	56	theme	pollution	313:321	arg1	problem					292:298	the frequent problem	279:298	the frequent problem of inorganic pollution	279:321	The development of industrialization and agricultural activities have carried various negative impacts to living organisms in recent decades and also, the frequent problem of inorganic pollution have been environmental anxiety to the community.					
33182083	4	57	theme	pH	794:795	arg1	function					759:766	a function	757:766	a function of contact time, solution pH and co-existing ions	757:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	57	theme	pH	794:795	arg1	mechanism					700:708	The adsorption mechanism	685:708	The adsorption mechanism of toxic Cr6+ and F-	685:729	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	6	58	theme	Langmuir	1364:1371	arg1	models					1382:1387	pseudo-second-order kinetic and Langmuir isotherm models	1332:1387	models	1382:1387	Additionally, the fabricated adsorbent is highly dependent on solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models.					
33182083	5	59	theme	scanning	961:968	arg1	microscopy					979:988	scanning electron microscopy	961:988	scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA)	961:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	60	with	calorimetry	1181:1191	arg1	BET					1115:1117	BET	1115:1117	BET	1115:1117	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	60	with	calorimetry	1181:1191	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	60	with	calorimetry	1181:1191	arg1	SEM-EDX					1033:1039	SEM-EDX	1033:1039	SEM-EDX	1033:1039	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	60	with	calorimetry	1181:1191	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	60	with	calorimetry	1181:1191	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	61	theme	access	909:914	arg1	properties					922:931	access their properties	909:931	access their properties	909:931	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	62	theme	X-ray	936:940	arg1	DSC					1194:1196	DSC	1194:1196	DSC	1194:1196	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	62	theme	X-ray	936:940	arg1	XRD					955:957	XRD	955:957	XRD	955:957	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	62	theme	X-ray	936:940	arg1	diffraction					942:952	X-ray diffraction	936:952	X-ray diffraction (XRD)	936:958	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	3	63	theme	biocompatible	520:532	arg1	zirconium					534:542	biocompatible zirconium	520:542	biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water	520:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	3	64	theme	adsorptive	633:642	arg1	removal					644:650	the adsorptive removal	629:650	the adsorptive removal of toxic Cr6+ and F- from water	629:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	5	65	with	diffraction	942:952	arg1	BET					1115:1117	BET	1115:1117	BET	1115:1117	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	65	with	diffraction	942:952	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	65	with	diffraction	942:952	arg1	SEM-EDX					1033:1039	SEM-EDX	1033:1039	SEM-EDX	1033:1039	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	65	with	diffraction	942:952	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	65	with	diffraction	942:952	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	4	66	theme	Cr6+	719:722	arg1	function					759:766	a function	757:766	a function of contact time, solution pH and co-existing ions	757:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	66	theme	Cr6+	719:722	arg1	mechanism					700:708	The adsorption mechanism	685:708	The adsorption mechanism of toxic Cr6+ and F-	685:729	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	0	67	from	water	121:125	arg1	sequestration					73:85	the sequestration	69:85	the sequestration of Chromium(VI) and fluoride from water	69:125	Facile synthesis of Zr4+ incorporated chitosan/gelatin composite for the sequestration of Chromium(VI) and fluoride from water.					
33182083	5	68	with	microscopy	979:988	arg1	BET					1115:1117	BET	1115:1117	BET	1115:1117	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	68	with	microscopy	979:988	arg1	spectroscopy					1070:1081	Fourier-transform infrared spectroscopy	1043:1081	Fourier-transform infrared spectroscopy (FTIR)	1043:1088	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	68	with	microscopy	979:988	arg1	SEM-EDX					1033:1039	SEM-EDX	1033:1039	SEM-EDX	1033:1039	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	68	with	microscopy	979:988	arg1	analysis					1139:1146	thermogravimetric analysis	1121:1146	thermogravimetric analysis (TGA)	1121:1152	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	5	68	with	microscopy	979:988	arg1	Spectroscopy					1019:1030	Energy Dispersive X-ray Spectroscopy	995:1030	Energy Dispersive X-ray Spectroscopy (SEM-EDX)	995:1040	The obtained materials were extensively studied by several physico-chemical techniques to access their properties by X-ray diffraction (XRD), scanning electron microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) analysis.					
33182083	3	69	theme	F-	670:671	arg1	removal					644:650	the adsorptive removal	629:650	the adsorptive removal of toxic Cr6+ and F- from water	629:682	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	4	70	theme	time	779:782	arg1	function					759:766	a function	757:766	a function of contact time, solution pH and co-existing ions	757:816	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	4	70	theme	time	779:782	arg1	mechanism					700:708	The adsorption mechanism	685:708	The adsorption mechanism of toxic Cr6+ and F-	685:729	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	3	71	attach	present	471:477	arg2	we					468:469	we	468:469	we	468:469	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	3	71	attach	present	471:477	arg1	work					462:465	this work	457:465	this work	457:465	In this work, we present a low-cost synthesis procedure to obtain biocompatible zirconium incorporated chitosan-gelatin composite (CS-Zr-GEL) were fabricated and explored for the adsorptive removal of toxic Cr6+ and F- from water.					
33182083	6	72	theme	isotherm	1373:1380	arg1	models					1382:1387	pseudo-second-order kinetic and Langmuir isotherm models	1332:1387	models	1382:1387	Additionally, the fabricated adsorbent is highly dependent on solution pH. The kinetic and isotherm data were fitted using pseudo-second-order kinetic and Langmuir isotherm models.					
33182083	4	73	theme	contact	771:777	arg1	time					779:782	contact time	771:782	contact time	771:782	The adsorption mechanism of toxic Cr6+ and F- was done by batch mode as a function of contact time, solution pH and co-existing ions.					
33182083	8	74	theme	water/wastewater	1629:1644	arg1	treatments					1646:1655	water/wastewater treatments	1629:1655	water/wastewater treatments	1629:1655	These findings demonstrate that the CS-Zr-GEL adsorbent represents a promising candidate that would have a practical influence on water/wastewater treatments.					
33182083	8	75	theme	promising	1568:1576	arg1	candidate					1578:1586	a promising candidate	1566:1586	a promising candidate that would have a practical influence on water/wastewater treatments	1566:1655	These findings demonstrate that the CS-Zr-GEL adsorbent represents a promising candidate that would have a practical influence on water/wastewater treatments.					
33038397	2	0	theme	electron	536:543	arg1	SEM					557:559	SEM	557:559	SEM	557:559	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	2	0	theme	electron	536:543	arg1	microscope					545:554	scanning electron microscope	527:554	scanning electron microscope (SEM)	527:560	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	0	1	with	films	35:39	arg1	compatibility					55:67	improved compatibility	46:67	improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx	46:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	5	2	from	separation	924:933	arg1	films					963:967	PLA/SiOx/CS films	951:967	PLA/SiOx/CS films	951:967	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	2	3	theme	scanning	527:534	arg1	SEM					557:559	SEM	557:559	SEM	557:559	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	2	3	theme	scanning	527:534	arg1	microscope					545:554	scanning electron microscope	527:554	scanning electron microscope (SEM)	527:560	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	5	4	theme	SEM	850:852	arg1	examination					854:864	SEM examination	850:864	SEM examination	850:864	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	6	5	dep	property	1090:1097	arg1	the					1071:1073	the	1071:1073	the	1071:1073	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	1	6	theme	transition	249:258	arg1	coating					154:160	A SiOx coating	147:160	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique	147:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	1	6	theme	transition	249:258	arg1	layer					260:264	a transition layer	247:264	a transition layer between polylactic acid (PLA) and chitosan (CS)	247:312	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	3	7	theme	PLA/SiOx/CS	619:629	arg1	films					631:635	PLA/SiOx/CS films	619:635	PLA/SiOx/CS films	619:635	The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated.					
33038397	6	8	theme	PLA/SiOx/CS	1129:1139	arg1	films					1141:1145	PLA/SiOx/CS films	1129:1145	PLA/SiOx/CS films	1129:1145	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	8	9	theme	composite	1334:1342	arg1	film					1344:1347	the composite film	1330:1347	the composite film	1330:1347	The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased.					
33038397	4	10	theme	PLA/SiOx/CS	804:814	arg1	films					816:820	PLA/SiOx/CS films	804:820	PLA/SiOx/CS films	804:820	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	4	11	theme	plasma	692:697	arg1	irradiation					699:709	oxygen plasma irradiation	685:709	oxygen plasma irradiation	685:709	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	0	12	theme	transition	119:128	arg1	coating					130:136	transition coating	119:136	transition coating of SiOx	119:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	1	13	theme	three-layer	324:334	arg1	film					346:349	a three-layer composite film	322:349	a three-layer composite film of PLA/SiOx/CS	322:364	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	2	14	dep	time	407:410	arg1	30s					418:420	30s	418:420	30s	418:420	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	2	14	dep	time	407:410	arg1	0 s					413:415	0 s	413:415	0 s	413:415	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	2	14	dep	time	407:410	arg1	60s					426:428	60s	426:428	60s	426:428	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	5	15	theme	interfacial	912:922	arg1	separation					924:933	the interfacial separation	908:933	the interfacial separation among layers in PLA/SiOx/CS films	908:967	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	8	16	theme	film	1344:1347	arg1	strength					1318:1325	The tensile strength	1306:1325	The tensile strength of the composite film	1306:1347	The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased.					
33038397	4	17	theme	films	816:820	arg1	SiOx-CS					793:799	SiOx-CS	793:799	SiOx-CS of PLA/SiOx/CS films	793:820	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	1	18	theme	composite	336:344	arg1	film					346:349	a three-layer composite film	322:349	a three-layer composite film of PLA/SiOx/CS	322:364	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	8	19	theme	tensile	1310:1316	arg1	strength					1318:1325	The tensile strength	1306:1325	The tensile strength of the composite film	1306:1347	The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased.					
33038397	6	20	theme	antibacterial	1103:1115	arg1	activity					1117:1124	antibacterial activity	1103:1124	antibacterial activity	1103:1124	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	1	21	theme	plasma-enhanced	175:189	arg1	technique					225:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	5	22	theme	intensified	1049:1059	arg1	SiOx					1008:1011	SiOx	1008:1011	SiOx	1008:1011	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	8	23	from	break	1411:1415	arg1	elongation					1397:1406	elongation	1397:1406	elongation at break	1397:1415	The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased.					
33038397	9	24	dep	applied	1492:1498	arg1	promising					1482:1490	promising	1482:1490	promising	1482:1490	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	1	25	theme	chemical	191:198	arg1	technique					225:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	1	26	theme	polylactic	274:283	arg1	PLA					291:293	PLA	291:293	PLA	291:293	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	1	26	theme	polylactic	274:283	arg1	acid					285:288	polylactic acid	274:288	polylactic acid (PLA)	274:294	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	9	27	with	films	1453:1457	arg1	multifunction					1464:1476	multifunction	1464:1476	multifunction	1464:1476	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	5	28	theme	PLA/CS	889:894	arg1	layers					896:901	PLA/CS layers	889:901	PLA/CS layers	889:901	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	1	29	theme	vapor	200:204	arg1	technique					225:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	0	30	theme	multilayer	24:33	arg1	films					35:39	biobased multilayer films	15:39	biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx	15:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	9	31	theme	prepared	1432:1439	arg1	films					1453:1457	The prepared PLA/SiOx/CS films	1428:1457	The prepared PLA/SiOx/CS films with multifunction	1428:1476	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	9	31	theme	prepared	1432:1439	arg1	materials					1530:1538	biobased materials	1521:1538	biobased materials	1521:1538	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	4	32	theme	oxygen	685:690	arg1	irradiation					699:709	oxygen plasma irradiation	685:709	oxygen plasma irradiation	685:709	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	1	33	theme	deposition	206:215	arg1	technique					225:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	0	34	theme	biobased	15:22	arg1	films					35:39	biobased multilayer films	15:39	biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx	15:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	0	35	theme	coating	130:136	arg1	function					107:114	a function	105:114	a function of transition coating of SiOx	105:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	3	36	theme	mechanical	567:576	arg1	properties					605:614	The mechanical, barrier and antibacterial properties	563:614	properties	605:614	The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated.					
33038397	6	37	theme	oxygen	1075:1080	arg1	property					1090:1097	oxygen barrier property	1075:1097	oxygen barrier property	1075:1097	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	2	38	theme	oxygen	381:386	arg1	time					407:410	oxygen plasma irradiation time	381:410	oxygen plasma irradiation time (0 s, 30s and 60s)	381:429	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	1	39	theme	PLA/SiOx/CS	354:364	arg1	film					346:349	a three-layer composite film	322:349	a three-layer composite film of PLA/SiOx/CS	322:364	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	5	40	theme	transition	988:997	arg1	layer					999:1003	the transition layer	984:1003	the transition layer of SiOx and oxygen plasma irradiation (60s) intensified	984:1059	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	2	41	from	effect	371:376	arg1	surface					439:445	SiOx surface	434:445	SiOx surface	434:445	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	2	41	from	effect	371:376	arg1	interface					458:466	PLA-CS interface	451:466	PLA-CS interface	451:466	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	1	42	theme	PECVD	218:222	arg1	technique					225:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	plasma-enhanced chemical vapor deposition (PECVD) technique	175:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	0	43	theme	films	35:39	arg1	Development					0:10	Development	0:10	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.	0:145	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	0	44	theme	SiOx	141:144	arg1	coating					130:136	transition coating	119:136	transition coating of SiOx	119:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	7	45	theme	PLA	1296:1298	arg1	film					1300:1303	the PLA film	1292:1303	the PLA film	1292:1303	Additionally, moisture resistance was slightly decreased following the incorporation of the CS coating compared with the PLA film.					
33038397	2	46	theme	time	407:410	arg1	effect					371:376	The effect	367:376	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface	367:466	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	7	47	theme	CS	1267:1268	arg1	coating					1270:1276	the CS coating	1263:1276	the CS coating	1263:1276	Additionally, moisture resistance was slightly decreased following the incorporation of the CS coating compared with the PLA film.					
33038397	2	48	theme	PLA-CS	451:456	arg1	interface					458:466	PLA-CS interface	451:466	PLA-CS interface	451:466	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	2	49	theme	pull-off	509:516	arg1	test					518:521	pull-off test	509:521	pull-off test	509:521	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	7	50	theme	moisture	1189:1196	arg1	resistance					1198:1207	moisture resistance	1189:1207	moisture resistance	1189:1207	Additionally, moisture resistance was slightly decreased following the incorporation of the CS coating compared with the PLA film.					
33038397	4	51	theme	adhesive	767:774	arg1	strength					776:783	the adhesive strength	763:783	the adhesive strength between SiOx-CS of PLA/SiOx/CS films	763:820	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	9	52	theme	PLA/SiOx/CS	1441:1451	arg1	films					1453:1457	The prepared PLA/SiOx/CS films	1428:1457	The prepared PLA/SiOx/CS films with multifunction	1428:1476	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	9	52	theme	PLA/SiOx/CS	1441:1451	arg1	materials					1530:1538	biobased materials	1521:1538	biobased materials	1521:1538	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	0	53	theme	improved	46:53	arg1	compatibility					55:67	improved compatibility	46:67	improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx	46:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	4	54	theme	time-dependent	827:840	arg1	manner					842:847	a time-dependent manner	825:847	a time-dependent manner	825:847	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	3	55	theme	antibacterial	591:603	arg1	properties					605:614	The mechanical, barrier and antibacterial properties	563:614	properties	605:614	The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated.					
33038397	8	56	theme	layers	1383:1388	arg1	number					1373:1378	the number	1369:1378	the number of layers	1369:1388	The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased.					
33038397	5	57	theme	PLA/SiOx/CS	951:961	arg1	films					963:967	PLA/SiOx/CS films	951:967	PLA/SiOx/CS films	951:967	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	9	58	theme	biobased	1521:1528	arg1	films					1453:1457	The prepared PLA/SiOx/CS films	1428:1457	The prepared PLA/SiOx/CS films with multifunction	1428:1476	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	9	58	theme	biobased	1521:1528	arg1	materials					1530:1538	biobased materials	1521:1538	biobased materials	1521:1538	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	1	59	used	used	239:242	arg2	coating					154:160	A SiOx coating	147:160	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique	147:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	1	59	used	used	239:242	arg2	layer					260:264	a transition layer	247:264	a transition layer between polylactic acid (PLA) and chitosan (CS)	247:312	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	4	60	theme	surface	751:757	arg1	hydrophilicity					724:737	the hydrophilicity	720:737	the hydrophilicity of the SiOx surface	720:757	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	4	60	theme	surface	751:757	arg1	strength					776:783	the adhesive strength	763:783	the adhesive strength between SiOx-CS of PLA/SiOx/CS films	763:820	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	6	61	theme	barrier	1082:1088	arg1	property					1090:1097	oxygen barrier property	1075:1097	oxygen barrier property	1075:1097	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	2	62	theme	contact	494:500	arg1	angle					502:506	the contact angle	490:506	the contact angle	490:506	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	3	63	theme	barrier	579:585	arg1	properties					605:614	The mechanical, barrier and antibacterial properties	563:614	properties	605:614	The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated.					
33038397	9	64	theme	food	1503:1506	arg1	packaging					1508:1516	food packaging	1503:1516	food packaging	1503:1516	The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.					
33038397	5	65	theme	oxygen	1017:1022	arg1	layer					999:1003	the transition layer	984:1003	the transition layer of SiOx and oxygen plasma irradiation (60s) intensified	984:1059	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	6	66	theme	films	1141:1145	arg1	property					1090:1097	oxygen barrier property	1075:1097	oxygen barrier property	1075:1097	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	6	66	theme	films	1141:1145	arg1	activity					1117:1124	antibacterial activity	1103:1124	antibacterial activity	1103:1124	Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced.					
33038397	0	67	theme	polylactic	77:86	arg1	acid-chitosan					88:100	polylactic acid-chitosan	77:100	polylactic acid-chitosan as a function of transition coating of SiOx	77:144	Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.					
33038397	5	68	theme	SiOx	1008:1011	arg1	layer					999:1003	the transition layer	984:1003	the transition layer of SiOx and oxygen plasma irradiation (60s) intensified	984:1059	SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified.					
33038397	4	69	theme	SiOx	746:749	arg1	surface					751:757	the SiOx surface	742:757	the SiOx surface	742:757	The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner.					
33038397	2	70	theme	plasma	388:393	arg1	time					407:410	oxygen plasma irradiation time	381:410	oxygen plasma irradiation time (0 s, 30s and 60s)	381:429	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	7	71	theme	coating	1270:1276	arg1	incorporation					1246:1258	the incorporation	1242:1258	the incorporation of the CS coating	1242:1276	Additionally, moisture resistance was slightly decreased following the incorporation of the CS coating compared with the PLA film.					
33038397	2	72	theme	SiOx	434:437	arg1	surface					439:445	SiOx surface	434:445	SiOx surface	434:445	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	3	73	theme	films	631:635	arg1	properties					605:614	The mechanical, barrier and antibacterial properties	563:614	properties	605:614	The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated.					
33038397	2	74	theme	irradiation	395:405	arg1	time					407:410	oxygen plasma irradiation time	381:410	oxygen plasma irradiation time (0 s, 30s and 60s)	381:429	The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM).					
33038397	1	75	theme	SiOx	149:152	arg1	layer					260:264	a transition layer	247:264	a transition layer between polylactic acid (PLA) and chitosan (CS)	247:312	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
33038397	1	75	theme	SiOx	149:152	arg1	coating					154:160	A SiOx coating	147:160	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique	147:233	A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS.					
32507220	0	0	theme	wheat	51:55	arg1	gelatinization					64:77	wheat starch gelatinization	51:77	wheat starch gelatinization	51:77	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	6	1	theme	solvent	1067:1073	arg1	capacity					1051:1058	the plasticization capacity	1032:1058	the plasticization capacity of the solvent	1032:1073	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	0	2	from	impact	4:9	arg1	gelatinization					64:77	wheat starch gelatinization	51:77	wheat starch gelatinization	51:77	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	1	3	from	effect	127:132	arg1	temperature					173:183	starch gelatinization temperature	151:183	starch gelatinization temperature	151:183	The effect of wheat bran on starch gelatinization temperature was investigated.					
32507220	7	4	dep	mechanism	1080:1088	arg1	behind					1090:1095	behind	1090:1095	behind our observations	1090:1112	The mechanism behind our observations was explained with the side-chain liquid-crystalline polymeric model for starch.					
32507220	4	5	dep	onset	662:666	arg1	temperatures					702:713	gelatinization temperatures	687:713	gelatinization temperatures	687:713	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	2	6	theme	Dynamic	203:209	arg1	sorption					224:231	Dynamic water vapour sorption	203:231	Dynamic water vapour sorption	203:231	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	6	7	theme	bran	966:969	arg1	components					971:980	the extractable wheat bran components	944:980	the extractable wheat bran components	944:980	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	6	7	theme	bran	966:969	arg1	phosphorus					1005:1014	phosphorus	1005:1014	phosphorus	1005:1014	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	6	7	theme	bran	966:969	arg1	potassium					991:999	potassium	991:999	potassium	991:999	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	0	8	theme	starch	57:62	arg1	gelatinization					64:77	wheat starch gelatinization	51:77	wheat starch gelatinization	51:77	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	6	9	theme	wheat	960:964	arg1	bran					966:969	extractable wheat bran	948:969	the extractable wheat bran components	944:980	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	7	10	theme	liquid-crystalline	1148:1165	arg1	model					1177:1181	the side-chain liquid-crystalline polymeric model	1133:1181	the side-chain liquid-crystalline polymeric model for starch	1133:1192	The mechanism behind our observations was explained with the side-chain liquid-crystalline polymeric model for starch.					
32507220	3	11	with	mixtures	397:404	arg1	calorimetry					433:443	differential scanning calorimetry	411:443	differential scanning calorimetry	411:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	5	12	theme	water	839:843	arg1	content					845:851	the water content	835:851	the water content	835:851	The conclusion temperature (Tc) was negatively affected by the water content.					
32507220	1	13	theme	wheat	137:141	arg1	bran					143:146	wheat bran	137:146	wheat bran	137:146	The effect of wheat bran on starch gelatinization temperature was investigated.					
32507220	6	14	theme	Fractionation	854:866	arg1	experiments					868:878	Fractionation experiments	854:878	Fractionation experiments	854:878	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	2	15	theme	capacity	253:260	arg1	experiments					262:272	Dynamic water vapour sorption and water retention capacity experiments	203:272	Dynamic water vapour sorption and water retention capacity experiments	203:272	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	1	16	theme	bran	143:146	arg1	effect					127:132	The effect	123:132	The effect of wheat bran on starch gelatinization temperature	123:183	The effect of wheat bran on starch gelatinization temperature was investigated.					
32507220	3	17	theme	starch	354:359	arg1	gelatinization					361:374	examining starch gelatinization	344:374	examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry	344:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	3	18	from	gelatinization	361:374	arg1	mixtures					397:404	starch-bran-water mixtures	379:404	starch-bran-water mixtures with differential scanning calorimetry	379:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	7	19	theme	polymeric	1167:1175	arg1	model					1177:1181	the side-chain liquid-crystalline polymeric model	1133:1181	the side-chain liquid-crystalline polymeric model for starch	1133:1192	The mechanism behind our observations was explained with the side-chain liquid-crystalline polymeric model for starch.					
32507220	3	20	theme	differential	411:422	arg1	calorimetry					433:443	differential scanning calorimetry	411:443	differential scanning calorimetry	411:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	6	21	theme	extractable	948:958	arg1	bran					966:969	extractable wheat bran	948:969	the extractable wheat bran components	944:980	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	2	22	dep	3	303:303	arg1	to					300:301	to	300:301	to	300:301	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	1	23	theme	starch	151:156	arg1	temperature					173:183	starch gelatinization temperature	151:183	starch gelatinization temperature	151:183	The effect of wheat bran on starch gelatinization temperature was investigated.					
32507220	0	24	theme	wheat	14:18	arg1	Triticum					21:28	Triticum	21:28	Triticum	21:28	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	0	24	theme	wheat	14:18	arg1	bran					43:46	wheat (Triticum aestivum L.) bran	14:46	wheat (Triticum aestivum L.) bran	14:46	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	0	25	theme	scanning	95:102	arg1	calorimetry					104:114	A differential scanning calorimetry	80:114	A differential scanning calorimetry study	80:120	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	4	26	from	concentration	752:764	arg1	water					769:773	water	769:773	water	769:773	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	1	27	theme	gelatinization	158:171	arg1	temperature					173:183	starch gelatinization temperature	151:183	starch gelatinization temperature	151:183	The effect of wheat bran on starch gelatinization temperature was investigated.					
32507220	3	28	theme	examining	344:352	arg1	gelatinization					361:374	examining starch gelatinization	344:374	examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry	344:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	0	29	theme	differential	82:93	arg1	calorimetry					104:114	A differential scanning calorimetry	80:114	A differential scanning calorimetry study	80:120	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	2	30	dep	more	311:314	arg1	water					316:320	water	316:320	water	316:320	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	4	31	theme	starch	612:617	arg1	behavior					634:641	starch gelatinization behavior	612:641	starch gelatinization behavior	612:641	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	2	32	theme	vapour	217:222	arg1	sorption					224:231	Dynamic water vapour sorption	203:231	Dynamic water vapour sorption	203:231	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	3	33	theme	scanning	424:431	arg1	calorimetry					433:443	differential scanning calorimetry	411:443	differential scanning calorimetry	411:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	0	34	theme	calorimetry	104:114	arg1	study					116:120	A differential scanning calorimetry study	80:120	A differential scanning calorimetry study	80:120	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	4	35	from	effect	575:580	arg1	behavior					634:641	starch gelatinization behavior	612:641	starch gelatinization behavior	612:641	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	2	36	theme	water	211:215	arg1	sorption					224:231	Dynamic water vapour sorption	203:231	Dynamic water vapour sorption	203:231	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	6	37	theme	plasticization	1036:1049	arg1	capacity					1051:1058	the plasticization capacity	1032:1058	the plasticization capacity of the solvent	1032:1073	Fractionation experiments demonstrated that the increased To and Tp were mainly caused by the extractable wheat bran components, such as potassium and phosphorus, which decrease the plasticization capacity of the solvent.					
32507220	0	38	dep	Triticum	21:28	arg1	L.					39:40	Triticum aestivum L.	21:40	Triticum aestivum L.	21:40	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	3	39	theme	starch-bran-water	379:395	arg1	mixtures					397:404	starch-bran-water mixtures	379:404	starch-bran-water mixtures with differential scanning calorimetry	379:443	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	2	40	theme	more	311:314	arg1	times					305:309	up to 3 times	297:309	up to 3 times more water than starch	297:332	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	7	41	theme	side-chain	1137:1146	arg1	model					1177:1181	the side-chain liquid-crystalline polymeric model	1133:1181	the side-chain liquid-crystalline polymeric model for starch	1133:1192	The mechanism behind our observations was explained with the side-chain liquid-crystalline polymeric model for starch.					
32507220	2	42	theme	retention	243:251	arg1	capacity					253:260	water retention capacity	237:260	water retention capacity	237:260	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	3	43	theme	substituting	471:482	arg1	starch					484:489	substituting starch	471:489	substituting starch	471:489	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	4	44	theme	mixture	589:595	arg1	composition					597:607	the mixture composition	585:607	the mixture composition	585:607	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	4	45	theme	bran	747:750	arg1	concentration					752:764	the bran concentration	743:764	the bran concentration in water	743:773	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	2	46	theme	water	237:241	arg1	capacity					253:260	water retention capacity	237:260	water retention capacity	237:260	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	3	47	theme	starch	484:489	arg1	effect					461:466	the effect	457:466	the effect of substituting starch by bran	457:497	However, examining starch gelatinization in starch-bran-water mixtures with differential scanning calorimetry showed that the effect of substituting starch by bran differed from that of moving into a regime of limiting water.					
32507220	4	48	theme	gelatinization	687:700	arg1	temperatures					702:713	gelatinization temperatures	687:713	gelatinization temperatures	687:713	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	0	49	theme	bran	43:46	arg1	impact					4:9	The impact	0:9	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization	0:77	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	4	50	theme	gelatinization	619:632	arg1	behavior					634:641	starch gelatinization behavior	612:641	starch gelatinization behavior	612:641	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
32507220	0	51	dep	study	116:120	arg1	impact					4:9	The impact	0:9	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization	0:77	The impact of wheat (Triticum aestivum L.) bran on wheat starch gelatinization: A differential scanning calorimetry study.					
32507220	2	52	theme	sorption	224:231	arg1	experiments					262:272	Dynamic water vapour sorption and water retention capacity experiments	203:272	Dynamic water vapour sorption and water retention capacity experiments	203:272	Dynamic water vapour sorption and water retention capacity experiments showed that bran bound up to 3 times more water than starch.					
32507220	5	53	theme	conclusion	780:789	arg1	temperature					791:801	The conclusion temperature	776:801	The conclusion temperature (Tc)	776:806	The conclusion temperature (Tc) was negatively affected by the water content.					
32507220	5	53	theme	conclusion	780:789	arg1	Tc					804:805	Tc	804:805	Tc	804:805	The conclusion temperature (Tc) was negatively affected by the water content.					
32507220	4	54	theme	composition	597:607	arg1	effect					575:580	the effect	571:580	the effect of the mixture composition on starch gelatinization behavior	571:641	Modelling the effect of the mixture composition on starch gelatinization behavior indicated that the onset (To) and peak (Tp) gelatinization temperatures were positively impacted by the bran concentration in water.					
33357865	7	0	theme	pure	1152:1155	arg1	>100 L m-2 h-1					1169:1182	>100 L m-2 h-1	1169:1182	>100 L m-2 h-1	1169:1182	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	7	0	theme	pure	1152:1155	arg1	flux					1163:1166	a higher pure water flux	1143:1166	a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar	1143:1227	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	7	1	from	behaviors	1270:1278	arg1	comparison					1283:1292	comparison	1283:1292	comparison to commercial PA membrane	1283:1318	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	4	2	theme	support	675:681	arg1	structure					683:691	porous support structure	668:691	porous support structure	668:691	Herein, the effect of chitosan-silver on porous support structure and filtration performance were systematically investigated.					
33357865	3	3	from	fouling	423:429	arg1	membranes					466:474	nanofiltration (NF) membranes	446:474	nanofiltration (NF) membranes	446:474	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	3	3	from	fouling	423:429	arg1	layer					437:441	PA layer	434:441	PA layer	434:441	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	0	4	theme	sustainable	76:86	arg1	alternative					88:98	a sustainable alternative	74:98	a sustainable alternative to prevent organic fouling	74:125	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	0	4	theme	sustainable	76:86	arg1	biopolymer					60:69	biopolymer	60:69	biopolymer	60:69	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	3	5	from	bio	407:409	arg1	membranes					466:474	nanofiltration (NF) membranes	446:474	nanofiltration (NF) membranes	446:474	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	3	5	from	bio	407:409	arg1	layer					437:441	PA layer	434:441	PA layer	434:441	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	1	6	from	layer	153:157	arg1	membranes					212:220	commercially successful thin film composite (TFC) membranes	162:220	commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	162:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	2	7	theme	fouling	340:346	arg1	propensity					348:357	its increasing fouling propensity	325:357	its increasing fouling propensity for highly contaminated waters	325:388	Lately, PA is under scrutiny due to its increasing fouling propensity for highly contaminated waters.					
33357865	6	8	theme	dye	1075:1077	arg1	terms					1017:1021	terms	1017:1021	terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %)	1017:1135	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	7	9	theme	PA	1308:1309	arg1	membrane					1311:1318	commercial PA membrane	1297:1318	commercial PA membrane	1297:1318	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	6	10	theme	wastewater	1099:1108	arg1	%					1134:1134	>98 %	1130:1134	>98 %	1130:1134	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	6	10	theme	wastewater	1099:1108	arg1	flux					1110:1113	tannery wastewater flux	1091:1113	tannery wastewater flux	1091:1113	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	7	11	theme	higher	1145:1150	arg1	>100 L m-2 h-1					1169:1182	>100 L m-2 h-1	1169:1182	>100 L m-2 h-1	1169:1182	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	7	11	theme	higher	1145:1150	arg1	flux					1163:1166	a higher pure water flux	1143:1166	a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar	1143:1227	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	5	12	theme	contact	898:904	arg1	angle					906:910	contact angle	898:910	contact angle	898:910	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	1	13	theme	challenging	271:281	arg1	task					283:286	challenging task	271:286	challenging task	271:286	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	4	14	theme	porous	668:673	arg1	structure					683:691	porous support structure	668:691	porous support structure	668:691	Herein, the effect of chitosan-silver on porous support structure and filtration performance were systematically investigated.					
33357865	6	15	theme	tannery	1091:1097	arg1	%					1134:1134	>98 %	1130:1134	>98 %	1130:1134	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	6	15	theme	tannery	1091:1097	arg1	flux					1110:1113	tannery wastewater flux	1091:1113	tannery wastewater flux	1091:1113	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	5	16	theme	ATR-IR	854:859	arg1	measurements					912:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	3	17	theme	present	491:497	arg1	study					499:503	present study	491:503	present study	491:503	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	3	18	theme	organic	415:421	arg1	fouling					423:429	organic fouling	415:429	organic fouling	415:429	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	6	19	theme	red	1057:1059	arg1	dye					1075:1077	red brown/organic dye	1057:1077	red brown/organic dye (>98 %)	1057:1085	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	6	19	theme	red	1057:1059	arg1	%					1084:1084	>98 %	1080:1084	>98 %	1080:1084	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	0	20	theme	organic	111:117	arg1	fouling					119:125	organic fouling	111:125	organic fouling	111:125	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	5	21	theme	angle	906:910	arg1	measurements					912:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	6	22	theme	brown/organic	1061:1073	arg1	dye					1075:1077	red brown/organic dye	1057:1077	red brown/organic dye (>98 %)	1057:1085	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	6	22	theme	brown/organic	1061:1073	arg1	%					1084:1084	>98 %	1080:1084	>98 %	1080:1084	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	3	23	theme	PA	434:435	arg1	layer					437:441	PA layer	434:441	PA layer	434:441	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	0	24	theme	thin	14:17	arg1	film					19:22	Restructuring thin film	0:22	Restructuring thin film composite membrane	0:41	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	7	25	theme	water	1157:1161	arg1	>100 L m-2 h-1					1169:1182	>100 L m-2 h-1	1169:1182	>100 L m-2 h-1	1169:1182	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	7	25	theme	water	1157:1161	arg1	flux					1163:1166	a higher pure water flux	1143:1166	a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar	1143:1227	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	3	26	dep	bio	407:409	arg1	the					403:405	the	403:405	the	403:405	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	3	27	theme	new	526:528	arg1	film					547:550	a new interfacial thin film	524:550	a new interfacial thin film asymmetric structure	524:571	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	1	28	theme	thin	186:189	arg1	film					191:194	commercially successful thin film composite	162:204	commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	162:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	1	28	theme	thin	186:189	arg1	TFC					207:209	TFC	207:209	TFC	207:209	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	0	29	theme	Restructuring	0:12	arg1	film					19:22	Restructuring thin film	0:22	Restructuring thin film composite membrane	0:41	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	3	30	theme	interfacial	530:540	arg1	film					547:550	a new interfacial thin film	524:550	a new interfacial thin film asymmetric structure	524:571	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	2	31	theme	contaminated	370:381	arg1	waters					383:388	highly contaminated waters	363:388	highly contaminated waters	363:388	Lately, PA is under scrutiny due to its increasing fouling propensity for highly contaminated waters.					
33357865	0	32	theme	composite	24:32	arg1	membrane					34:41	Restructuring thin film composite membrane	0:41	Restructuring thin film composite membrane	0:41	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	6	33	theme	flow	986:989	arg1	process					991:997	cross flow process	980:997	cross flow process	980:997	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	6	34	theme	flux	1110:1113	arg1	terms					1017:1021	terms	1017:1021	terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %)	1017:1135	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	8	35	theme	surface	1330:1336	arg1	characteristics					1338:1352	surface characteristics	1330:1352	surface characteristics of the membranes before and after rigorous testing	1330:1403	Further, surface characteristics of the membranes before and after rigorous testing were evaluated using AFM micrographs and SEM imaging.					
33357865	1	36	theme	composite	196:204	arg1	film					191:194	commercially successful thin film composite	162:204	commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	162:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	1	36	theme	composite	196:204	arg1	TFC					207:209	TFC	207:209	TFC	207:209	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	0	37	theme	film	19:22	arg1	membrane					34:41	Restructuring thin film composite membrane	0:41	Restructuring thin film composite membrane	0:41	Restructuring thin film composite membrane interfaces using biopolymer as a sustainable alternative to prevent organic fouling.					
33357865	6	38	theme	cross	980:984	arg1	process					991:997	cross flow process	980:997	cross flow process	980:997	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	8	39	theme	rigorous	1388:1395	arg1	testing					1397:1403	rigorous testing	1388:1403	rigorous testing	1388:1403	Further, surface characteristics of the membranes before and after rigorous testing were evaluated using AFM micrographs and SEM imaging.					
33357865	3	40	theme	long	481:484	arg1	run					486:488	a long run	479:488	a long run	479:488	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	5	41	theme	AFM	869:871	arg1	measurements					912:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	4	42	from	effect	639:644	arg1	structure					683:691	porous support structure	668:691	porous support structure	668:691	Herein, the effect of chitosan-silver on porous support structure and filtration performance were systematically investigated.					
33357865	4	42	from	effect	639:644	arg1	performance					708:718	filtration performance	697:718	filtration performance	697:718	Herein, the effect of chitosan-silver on porous support structure and filtration performance were systematically investigated.					
33357865	3	43	theme	asymmetric	552:561	arg1	structure					563:571	a new interfacial thin film asymmetric structure	524:571	a new interfacial thin film asymmetric structure	524:571	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	1	44	theme	film	191:194	arg1	membranes					212:220	commercially successful thin film composite (TFC) membranes	162:220	commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	162:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	6	45	theme	NaCl	1043:1046	arg1	terms					1017:1021	terms	1017:1021	terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %)	1017:1135	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	7	46	theme	antifouling	1258:1268	arg1	behaviors					1270:1278	exceptional antifouling behaviors	1246:1278	exceptional antifouling behaviors in comparison to commercial PA membrane	1246:1318	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	3	47	theme	sustainable	602:612	arg1	alternative					614:624	sustainable alternative	602:624	sustainable alternative	602:624	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	5	48	theme	surface	824:830	arg1	characteristics					832:846	surface characteristics	824:846	surface characteristics	824:846	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	5	49	theme	FESEM	862:866	arg1	measurements					912:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	5	50	theme	spectroscopy	881:892	arg1	measurements					912:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements	854:923	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	3	51	theme	thin	542:545	arg1	film					547:550	a new interfacial thin film	524:550	a new interfacial thin film asymmetric structure	524:571	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	7	52	from	4 bar	1223:1227	arg1	>100 L m-2 h-1					1169:1182	>100 L m-2 h-1	1169:1182	>100 L m-2 h-1	1169:1182	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	7	52	from	4 bar	1223:1227	arg1	flux					1163:1166	a higher pure water flux	1143:1166	a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar	1143:1227	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	2	53	theme	increasing	329:338	arg1	propensity					348:357	its increasing fouling propensity	325:357	its increasing fouling propensity for highly contaminated waters	325:388	Lately, PA is under scrutiny due to its increasing fouling propensity for highly contaminated waters.					
33357865	6	54	theme	asymmetric	944:953	arg1	performances					964:975	New asymmetric membrane performances	940:975	New asymmetric membrane performances in cross flow process	940:997	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	7	55	theme	exceptional	1246:1256	arg1	behaviors					1270:1278	exceptional antifouling behaviors	1246:1278	exceptional antifouling behaviors in comparison to commercial PA membrane	1246:1318	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	3	56	theme	film	547:550	arg1	structure					563:571	a new interfacial thin film asymmetric structure	524:571	a new interfacial thin film asymmetric structure	524:571	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	1	57	theme	Replacing	128:136	arg1	layer					153:157	Replacing polyamide (PA) layer	128:157	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	128:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	6	58	theme	New	940:942	arg1	performances					964:975	New asymmetric membrane performances	940:975	New asymmetric membrane performances in cross flow process	940:997	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	6	59	from	performances	964:975	arg1	process					991:997	cross flow process	980:997	cross flow process	980:997	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	8	60	theme	membranes	1361:1369	arg1	characteristics					1338:1352	surface characteristics	1330:1352	surface characteristics of the membranes before and after rigorous testing	1330:1403	Further, surface characteristics of the membranes before and after rigorous testing were evaluated using AFM micrographs and SEM imaging.					
33357865	1	61	theme	polyamide	138:146	arg1	layer					153:157	Replacing polyamide (PA) layer	128:157	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	128:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	5	62	theme	UV-vis	874:879	arg1	spectroscopy					881:892	UV-vis spectroscopy	874:892	UV-vis spectroscopy	874:892	Further, the membranes were characterized for their functionality and surface characteristics using ATR-IR, FESEM, AFM, UV-vis spectroscopy and contact angle measurements, respectively.					
33357865	6	63	theme	flux	1037:1040	arg1	terms					1017:1021	terms	1017:1021	terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %)	1017:1135	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	7	64	theme	commercial	1297:1306	arg1	membrane					1311:1318	commercial PA membrane	1297:1318	commercial PA membrane	1297:1318	With a higher pure water flux (>100 L m-2 h-1) compared to control (40 L m-2 h-1) at 4 bar, membrane showed exceptional antifouling behaviors in comparison to commercial PA membrane.					
33357865	4	65	theme	filtration	697:706	arg1	performance					708:718	filtration performance	697:718	filtration performance	697:718	Herein, the effect of chitosan-silver on porous support structure and filtration performance were systematically investigated.					
33357865	6	66	theme	water	1031:1035	arg1	flux					1037:1040	pure water flux	1026:1040	pure water flux	1026:1040	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	8	67	theme	AFM	1426:1428	arg1	micrographs					1430:1440	AFM micrographs	1426:1440	AFM micrographs	1426:1440	Further, surface characteristics of the membranes before and after rigorous testing were evaluated using AFM micrographs and SEM imaging.					
33357865	3	68	theme	nanofiltration	446:459	arg1	membranes					466:474	nanofiltration (NF) membranes	446:474	nanofiltration (NF) membranes	446:474	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	1	69	theme	PA	149:150	arg1	layer					153:157	Replacing polyamide (PA) layer	128:157	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	128:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	1	70	theme	successful	175:184	arg1	film					191:194	commercially successful thin film composite	162:204	commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization	162:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	1	70	theme	successful	175:184	arg1	TFC					207:209	TFC	207:209	TFC	207:209	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
33357865	6	71	theme	pure	1026:1029	arg1	flux					1037:1040	pure water flux	1026:1040	pure water flux	1026:1040	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	8	72	theme	SEM	1446:1448	arg1	imaging					1450:1456	SEM imaging	1446:1456	SEM imaging	1446:1456	Further, surface characteristics of the membranes before and after rigorous testing were evaluated using AFM micrographs and SEM imaging.					
33357865	3	73	theme	biopolymer	579:588	arg1	chitosan					590:597	biopolymer chitosan	579:597	biopolymer chitosan	579:597	To mitigate the bio and organic fouling on PA layer in nanofiltration (NF) membranes in a long run, present study attempts to create a new interfacial thin film asymmetric structure using biopolymer chitosan as sustainable alternative.					
33357865	4	74	theme	chitosan-silver	649:663	arg1	effect					639:644	the effect	635:644	the effect of chitosan-silver on porous support structure and filtration performance	635:718	Herein, the effect of chitosan-silver on porous support structure and filtration performance were systematically investigated.					
33357865	6	75	theme	membrane	955:962	arg1	performances					964:975	New asymmetric membrane performances	940:975	New asymmetric membrane performances in cross flow process	940:997	New asymmetric membrane performances in cross flow process were evaluated in terms of pure water flux, NaCl (∼40 %), red brown/organic dye (>98 %) and tannery wastewater flux and rejection (>98 %).					
33357865	1	76	theme	interfacial	235:245	arg1	polymerization					247:260	interfacial polymerization	235:260	interfacial polymerization	235:260	Replacing polyamide (PA) layer in commercially successful thin film composite (TFC) membranes prepared via interfacial polymerization has been challenging task.					
32324770	1	0	located	found	201:205	arg1	plant					210:214	plant	210:214	plant	210:214	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	0	located	found	201:205	arg1	glycoproteins					227:239	insect glycoproteins	220:239	insect glycoproteins	220:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	0	located	found	201:205	arg2	epitopes					286:293	epitopes	286:293	epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results	286:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	0	located	found	201:205	arg2	structures					190:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures	133:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins	133:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	1	theme	diagnostic	360:369	arg1	results					386:392	obscure in vitro diagnostic (IVD) serology results	343:392	obscure in vitro diagnostic (IVD) serology results	343:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	14	2	theme	positive	2460:2467	arg1	sera					2489:2492	positive CCD-sIgE containing sera	2460:2492	positive CCD-sIgE containing sera	2460:2492	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	11	3	theme	CCD	1910:1912	arg1	interference					1914:1925	CCD interference	1910:1925	CCD interference	1910:1925	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	13	4	theme	non-glycosylated	2206:2221	arg1	components					2246:2255	non-glycosylated recombinant allergenic components	2206:2255	non-glycosylated recombinant allergenic components coupled to cellulose matrices	2206:2285	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32324770	12	5	theme	allergen-sIgE	2048:2060	arg1	results					2062:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	4	6	theme	in	900:901	arg1	platform					924:931	a commonly-used in vitro sIgE automated platform	884:931	a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components	884:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	7	7	theme	sIgE	1495:1498	arg1	NOVEOS					1455:1460	NOVEOS	1455:1460	NOVEOS	1455:1460	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	7	7	theme	sIgE	1495:1498	arg1	assay					1500:1504	a non-cellulose based automated sIgE assay	1463:1504	a non-cellulose based automated sIgE assay	1463:1504	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	14	8	theme	inhibitor	2447:2455	arg1	addition					2421:2428	the addition	2417:2428	the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera	2417:2492	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	10	9	theme	false-positive	1796:1809	arg1	results					1811:1817	false-positive results	1796:1817	false-positive results	1796:1817	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	5	10	from	patients	1037:1044	arg1	Sera					1027:1030	METHODS Sera	1019:1030	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass	1019:1101	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	3	11	dep	in	692:693	arg1	vitro					695:699	vitro	695:699	vitro	695:699	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	6	12	dep	absence	1363:1369	arg1	the					1359:1361	the	1359:1361	the	1359:1361	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	14	13	theme	sIgE	2542:2545	arg1	assay					2547:2551	a non-cellulose based sIgE assay	2520:2551	a non-cellulose based sIgE assay	2520:2551	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	6	14	theme	Positive	1228:1235	arg1	sera					1246:1249	Positive CCD-sIgE sera	1228:1249	Positive CCD-sIgE sera	1228:1249	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	10	15	dep	kU/L	1873:1876	arg1	to					1878:1879	to	1878:1879	to	1878:1879	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	6	16	theme	ImmunoCAP	1334:1342	arg1	phase					1350:1354	the ImmunoCAP solid phase	1330:1354	the ImmunoCAP solid phase	1330:1354	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	14	17	theme	soluble	2435:2441	arg1	inhibitor					2447:2455	a soluble CCD inhibitor	2433:2455	a soluble CCD inhibitor	2433:2455	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	12	18	theme	CONCLUSION	2009:2018	arg1	results					2062:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	14	19	theme	non-cellulose	2522:2534	arg1	assay					2547:2551	a non-cellulose based sIgE assay	2520:2551	a non-cellulose based sIgE assay	2520:2551	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	4	20	theme	interference	868:879	arg1	prevalence					845:854	the prevalence	841:854	the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components	841:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	3	21	theme	traditional	758:768	arg1	testing					784:790	traditional extract-based testing	758:790	traditional extract-based testing	758:790	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	12	22	theme	elevated	2028:2035	arg1	CONCLUSION					2009:2018	CONCLUSION	2009:2018	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	9	23	theme	non-glycosylated	1718:1733	arg1	allergosorbents					1735:1749	non-glycosylated allergosorbents	1718:1749	non-glycosylated allergosorbents that were negative on NOVEOS	1718:1778	Of those positives, 17% resulted in CCD-sIgE-positive (false positive) results on ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS.					
32324770	12	24	theme	carbohydrate	2148:2159	arg1	allergosorbent					2174:2187	a carbohydrate matrix-based allergosorbent	2146:2187	a carbohydrate matrix-based allergosorbent	2146:2187	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	3	25	theme	CRD	749:751	arg1	advantage					736:744	the advantage	732:744	the advantage of CRD over traditional extract-based testing	732:790	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	6	26	theme	inhibitor	1401:1409	arg1	presence					1375:1382	presence	1375:1382	presence	1375:1382	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	6	26	theme	inhibitor	1401:1409	arg1	absence					1363:1369	absence	1363:1369	absence	1363:1369	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	3	27	theme	recent	585:590	arg1	study					592:596	a recent study	583:596	a recent study	583:596	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	4	28	theme	cellulose-based	949:963	arg1	matrix					965:970	a cellulose-based matrix	947:970	a cellulose-based matrix	947:970	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	6	29	theme	soluble	1389:1395	arg1	inhibitor					1401:1409	a soluble CCD inhibitor	1387:1409	a soluble CCD inhibitor	1387:1409	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	10	30	theme	CCD-sIgE	1854:1861	arg1	levels					1844:1849	varying levels	1836:1849	varying levels of CCD-sIgE	1836:1861	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	4	31	theme	sIgE	909:912	arg1	platform					924:931	a commonly-used in vitro sIgE automated platform	884:931	a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components	884:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	6	32	theme	recombinant	1298:1308	arg1	components					1310:1319	non-glycosylated recombinant components	1281:1319	non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor	1281:1409	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	13	33	theme	components	2246:2255	arg1	use					2199:2201	Even the use	2190:2201	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices	2190:2285	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32324770	1	34	theme	determinant	172:182	arg1	structures					190:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures	133:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins	133:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	34	theme	determinant	172:182	arg1	epitopes					286:293	epitopes	286:293	epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results	286:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	35	theme	CCD	185:187	arg1	structures					190:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures	133:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins	133:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	35	theme	CCD	185:187	arg1	epitopes					286:293	epitopes	286:293	epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results	286:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	2	36	theme	CCD-specific	539:550	arg1	detection					563:571	CCD-specific IgE (sIgE) detection	539:571	CCD-specific IgE (sIgE) detection	539:571	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	1	37	theme	IVD	372:374	arg1	results					386:392	obscure in vitro diagnostic (IVD) serology results	343:392	obscure in vitro diagnostic (IVD) serology results	343:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	10	38	theme	0.67	1868:1871	arg1	kU/L					1873:1876	kU/L	1873:1876	kU/L	1873:1876	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	8	39	theme	sensitized	1536:1545	arg1	population					1547:1556	the sensitized population	1532:1556	the sensitized population tested in this study	1532:1577	RESULTS Sera from 35% of the sensitized population tested in this study were positive (≥0.35 kU/L) for CCD-sIgE.					
32324770	12	40	theme	CCD-sIgE	2112:2119	arg1	presence					2100:2107	the presence	2096:2107	the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent	2096:2187	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	0	41	theme	recombinant	100:110	arg1	components					121:130	recombinant allergen components	100:130	recombinant allergen components	100:130	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	1	42	theme	serology	377:384	arg1	results					386:392	obscure in vitro diagnostic (IVD) serology results	343:392	obscure in vitro diagnostic (IVD) serology results	343:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	14	43	theme	quantitative	2372:2383	arg1	results					2389:2395	quantitative IgE results	2372:2395	quantitative IgE results	2372:2395	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	5	44	theme	CCD-sIgE	1121:1128	arg1	reactivity					1130:1139	CCD-sIgE reactivity	1121:1139	CCD-sIgE reactivity	1121:1139	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	4	45	theme	CCD-free	986:993	arg1	components					1007:1016	CCD-free recombinant components	986:1016	CCD-free recombinant components	986:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	13	46	theme	allergenic	2235:2244	arg1	components					2246:2255	non-glycosylated recombinant allergenic components	2206:2255	non-glycosylated recombinant allergenic components coupled to cellulose matrices	2206:2285	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32324770	0	47	theme	cellulose-based	56:70	arg1	tests					84:88	cellulose-based IgE allergy tests	56:88	cellulose-based IgE allergy tests	56:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	5	48	theme	METHODS	1019:1025	arg1	Sera					1027:1030	METHODS Sera	1019:1030	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass	1019:1101	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	13	49	theme	detection	2315:2323	arg1	risk					2307:2310	their risk	2301:2310	their risk of detection	2301:2323	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32324770	14	50	theme	CCD	2338:2340	arg1	interference					2342:2353	CCD interference	2338:2353	CCD interference that compromises quantitative IgE results	2338:2395	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	2	51	theme	component	420:428	arg1	CRD					450:452	CRD	450:452	CRD	450:452	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	2	51	theme	component	420:428	arg1	diagnosis					439:447	component resolved diagnosis	420:447	component resolved diagnosis (CRD)	420:453	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	0	52	theme	Cross-reactive	0:13	arg1	interference					40:51	Cross-reactive carbohydrate determinant interference	0:51	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests	0:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	1	53	theme	extensive	312:320	arg1	cross-reactivity					322:337	extensive cross-reactivity	312:337	extensive cross-reactivity	312:337	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	2	54	theme	non-glycosylated	468:483	arg1	components					485:494	recombinant non-glycosylated components	456:494	recombinant non-glycosylated components	456:494	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	0	55	theme	determinant	28:38	arg1	interference					40:51	Cross-reactive carbohydrate determinant interference	0:51	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests	0:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	7	56	theme	based	1479:1483	arg1	NOVEOS					1455:1460	NOVEOS	1455:1460	NOVEOS	1455:1460	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	7	56	theme	based	1479:1483	arg1	assay					1500:1504	a non-cellulose based automated sIgE assay	1463:1504	a non-cellulose based automated sIgE assay	1463:1504	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	1	57	theme	Cross-reactive	144:157	arg1	structures					190:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures	133:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins	133:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	57	theme	Cross-reactive	144:157	arg1	epitopes					286:293	epitopes	286:293	epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results	286:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	11	58	theme	IgE	1971:1973	arg1	kUA/L					2001:2005	0.35 kUA/L- 3.00 kUA/L	1984:2005	0.35 kUA/L- 3.00 kUA/L	1984:2005	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	11	58	theme	IgE	1971:1973	arg1	results					1975:1981	low-positive IgE results	1958:1981	low-positive IgE results (0.35 kUA/L- 3.00 kUA/L)	1958:2006	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	1	59	theme	in	351:352	arg1	results					386:392	obscure in vitro diagnostic (IVD) serology results	343:392	obscure in vitro diagnostic (IVD) serology results	343:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	5	60	dep	ImmunoCAP/Phadia	1144:1159	arg1	autoanalyzers					1172:1184	autoanalyzers	1172:1184	autoanalyzers against the MUXF3 carbohydrate component	1172:1225	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	3	61	theme	solid	657:661	arg1	matrix					669:674	the cellulose solid phase matrix	643:674	the cellulose solid phase matrix used in certain in vitro diagnostic assays	643:717	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	0	62	theme	IgE	72:74	arg1	tests					84:88	cellulose-based IgE allergy tests	56:88	cellulose-based IgE allergy tests	56:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	5	63	theme	carbohydrate	1204:1215	arg1	component					1217:1225	the MUXF3 carbohydrate component	1194:1225	the MUXF3 carbohydrate component	1194:1225	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	0	64	from	interference	40:51	arg1	tests					84:88	cellulose-based IgE allergy tests	56:88	cellulose-based IgE allergy tests	56:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	5	65	theme	silver	1068:1073	arg1	birch					1075:1079	silver birch	1068:1079	silver birch	1068:1079	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	3	66	theme	in	692:693	arg1	assays					712:717	certain in vitro diagnostic assays	684:717	certain in vitro diagnostic assays	684:717	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	13	67	gly	non-glycosylated	2206:2221	arg1	components					2246:2255	non-glycosylated recombinant allergenic components	2206:2255	non-glycosylated recombinant allergenic components coupled to cellulose matrices	2206:2285	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32324770	1	68	theme	IgE	268:270	arg1	antibodies					272:281	IgE antibodies	268:281	IgE antibodies	268:281	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	2	69	dep	diagnosis	439:447	arg1	the					400:402	the	400:402	the	400:402	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	2	69	dep	diagnosis	439:447	arg1	introduction					404:415	introduction	404:415	introduction	404:415	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	11	70	theme	interference	1914:1925	arg1	incidence					1897:1905	The incidence	1893:1905	The incidence of CCD interference	1893:1925	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	2	71	gly	non-glycosylated	468:483	arg1	components					485:494	recombinant non-glycosylated components	456:494	recombinant non-glycosylated components	456:494	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	2	72	used	utilized	506:513	arg2	components					485:494	recombinant non-glycosylated components	456:494	recombinant non-glycosylated components	456:494	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	10	73	contain	had	1832:1834	arg2	levels					1844:1849	varying levels	1836:1849	varying levels of CCD-sIgE	1836:1861	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	10	73	contain	had	1832:1834	arg1	Sera					1781:1784	Sera	1781:1784	Sera producing false-positive results on ImmunoCAP	1781:1830	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	14	74	theme	CCD-sIgE	2469:2476	arg1	sera					2489:2492	positive CCD-sIgE containing sera	2460:2492	positive CCD-sIgE containing sera	2460:2492	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	3	75	theme	diagnostic	701:710	arg1	assays					712:717	certain in vitro diagnostic assays	684:717	certain in vitro diagnostic assays	684:717	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	1	76	gly	glycoproteins	227:239	arg1	glycoproteins					227:239	insect glycoproteins	220:239	insect glycoproteins	220:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	13	77	theme	recombinant	2223:2233	arg1	components					2246:2255	non-glycosylated recombinant allergenic components	2206:2255	non-glycosylated recombinant allergenic components coupled to cellulose matrices	2206:2285	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32324770	12	78	theme	diagnostic	2037:2046	arg1	results					2062:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results	2009:2068	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	4	79	theme	commonly-used	886:898	arg1	platform					924:931	a commonly-used in vitro sIgE automated platform	884:931	a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components	884:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	6	80	theme	CCD-sIgE	1237:1244	arg1	sera					1246:1249	Positive CCD-sIgE sera	1228:1249	Positive CCD-sIgE sera	1228:1249	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	6	81	gly	non-glycosylated	1281:1296	arg1	components					1310:1319	non-glycosylated recombinant components	1281:1319	non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor	1281:1409	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	6	82	theme	solid	1344:1348	arg1	phase					1350:1354	the ImmunoCAP solid phase	1330:1354	the ImmunoCAP solid phase	1330:1354	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	14	83	theme	CCD	2443:2445	arg1	inhibitor					2447:2455	a soluble CCD inhibitor	2433:2455	a soluble CCD inhibitor	2433:2455	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	9	84	gly	non-glycosylated	1718:1733	arg1	allergosorbents					1735:1749	non-glycosylated allergosorbents	1718:1749	non-glycosylated allergosorbents that were negative on NOVEOS	1718:1778	Of those positives, 17% resulted in CCD-sIgE-positive (false positive) results on ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS.					
32324770	14	85	theme	based	2536:2540	arg1	assay					2547:2551	a non-cellulose based sIgE assay	2520:2551	a non-cellulose based sIgE assay	2520:2551	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	7	86	theme	automated	1485:1493	arg1	NOVEOS					1455:1460	NOVEOS	1455:1460	NOVEOS	1455:1460	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	7	86	theme	automated	1485:1493	arg1	assay					1500:1504	a non-cellulose based automated sIgE assay	1463:1504	a non-cellulose based automated sIgE assay	1463:1504	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	8	87	dep	RESULTS	1507:1513	arg1	positive					1584:1591	positive	1584:1591	positive	1584:1591	RESULTS Sera from 35% of the sensitized population tested in this study were positive (≥0.35 kU/L) for CCD-sIgE.					
32324770	10	88	theme	varying	1836:1842	arg1	levels					1844:1849	varying levels	1836:1849	varying levels of CCD-sIgE	1836:1861	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	1	89	theme	insect	220:225	arg1	glycoproteins					227:239	insect glycoproteins	220:239	insect glycoproteins	220:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	4	90	theme	CCD-sIgE	859:866	arg1	interference					868:879	CCD-sIgE interference	859:879	CCD-sIgE interference	859:879	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	7	91	theme	non-cellulose	1465:1477	arg1	NOVEOS					1455:1460	NOVEOS	1455:1460	NOVEOS	1455:1460	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	7	91	theme	non-cellulose	1465:1477	arg1	assay					1500:1504	a non-cellulose based automated sIgE assay	1463:1504	a non-cellulose based automated sIgE assay	1463:1504	For comparison, sera were then analyzed on NOVEOS, a non-cellulose based automated sIgE assay.					
32324770	3	92	theme	extract-based	770:782	arg1	testing					784:790	traditional extract-based testing	758:790	traditional extract-based testing	758:790	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	9	93	from	results	1691:1697	arg1	ImmunoCAP					1702:1710	ImmunoCAP	1702:1710	ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS	1702:1778	Of those positives, 17% resulted in CCD-sIgE-positive (false positive) results on ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS.					
32324770	9	94	dep	CCD-sIgE-positive	1656:1672	arg1	positive					1681:1688	positive	1681:1688	positive	1681:1688	Of those positives, 17% resulted in CCD-sIgE-positive (false positive) results on ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS.					
32324770	6	95	theme	CCD	1397:1399	arg1	inhibitor					1401:1409	a soluble CCD inhibitor	1387:1409	a soluble CCD inhibitor	1387:1409	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	2	96	theme	detection	563:571	arg1	risk					531:534	the risk	527:534	the risk of CCD-specific IgE (sIgE) detection	527:571	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	12	97	theme	matrix-based	2161:2172	arg1	allergosorbent					2174:2187	a carbohydrate matrix-based allergosorbent	2146:2187	a carbohydrate matrix-based allergosorbent	2146:2187	CONCLUSION Falsely elevated diagnostic allergen-sIgE results can commonly occur due to the presence of CCD-sIgE using assays that employ a carbohydrate matrix-based allergosorbent.					
32324770	11	98	theme	kUA/L-	1989:1994	arg1	kUA/L					2001:2005	0.35 kUA/L- 3.00 kUA/L	1984:2005	0.35 kUA/L- 3.00 kUA/L	1984:2005	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	11	98	theme	kUA/L-	1989:1994	arg1	results					1975:1981	low-positive IgE results	1958:1981	low-positive IgE results (0.35 kUA/L- 3.00 kUA/L)	1958:2006	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	1	99	theme	carbohydrate	159:170	arg1	structures					190:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures	133:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins	133:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	99	theme	carbohydrate	159:170	arg1	epitopes					286:293	epitopes	286:293	epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results	286:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	6	100	theme	non-glycosylated	1281:1296	arg1	components					1310:1319	non-glycosylated recombinant components	1281:1319	non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor	1281:1409	Positive CCD-sIgE sera were further analyzed against non-glycosylated recombinant components bound to the ImmunoCAP solid phase in the absence and presence of a soluble CCD inhibitor.					
32324770	10	101	theme	36.52	1881:1885	arg1	kU/L					1873:1876	kU/L	1873:1876	kU/L	1873:1876	Sera producing false-positive results on ImmunoCAP had varying levels of CCD-sIgE from 0.67 kU/L to 36.52 kU/L.					
32324770	4	102	theme	automated	914:922	arg1	platform					924:931	a commonly-used in vitro sIgE automated platform	884:931	a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components	884:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	5	103	theme	timothy	1089:1095	arg1	grass					1097:1101	timothy grass	1089:1101	timothy grass	1089:1101	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	4	104	theme	recombinant	995:1005	arg1	components					1007:1016	CCD-free recombinant components	986:1016	CCD-free recombinant components	986:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	8	105	theme	population	1547:1556	arg1	%					1527:1527	35%	1525:1527	35% of the sensitized population tested in this study	1525:1577	RESULTS Sera from 35% of the sensitized population tested in this study were positive (≥0.35 kU/L) for CCD-sIgE.					
32324770	8	105	theme	population	1547:1556	arg1	population					1547:1556	the sensitized population	1532:1556	the sensitized population tested in this study	1532:1577	RESULTS Sera from 35% of the sensitized population tested in this study were positive (≥0.35 kU/L) for CCD-sIgE.					
32324770	8	106	dep	positive	1584:1591	arg1	kU/L					1600:1603	≥0.35 kU/L	1594:1603	≥0.35 kU/L	1594:1603	RESULTS Sera from 35% of the sensitized population tested in this study were positive (≥0.35 kU/L) for CCD-sIgE.					
32324770	0	107	theme	allergen	112:119	arg1	components					121:130	recombinant allergen components	100:130	recombinant allergen components	100:130	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	14	108	theme	IgE	2385:2387	arg1	results					2389:2395	quantitative IgE results	2372:2395	quantitative IgE results	2372:2395	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	2	109	theme	sIgE	557:560	arg1	detection					563:571	CCD-specific IgE (sIgE) detection	539:571	CCD-specific IgE (sIgE) detection	539:571	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	14	110	dep	such	2554:2557	arg1	as					2559:2560	as	2559:2560	as	2559:2560	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	8	111	from	%	1527:1527	arg1	Sera					1515:1518	Sera	1515:1518	Sera from 35% of the sensitized population tested in this study	1515:1577	RESULTS Sera from 35% of the sensitized population tested in this study were positive (≥0.35 kU/L) for CCD-sIgE.					
32324770	2	112	theme	IgE	552:554	arg1	detection					563:571	CCD-specific IgE (sIgE) detection	539:571	CCD-specific IgE (sIgE) detection	539:571	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	1	113	dep	in	351:352	arg1	vitro					354:358	vitro	354:358	vitro	354:358	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	14	114	theme	interference	2342:2353	arg1	risk					2330:2333	The risk	2326:2333	The risk of CCD interference that compromises quantitative IgE results	2326:2395	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	2	115	theme	resolved	430:437	arg1	CRD					450:452	CRD	450:452	CRD	450:452	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	2	115	theme	resolved	430:437	arg1	diagnosis					439:447	component resolved diagnosis	420:447	component resolved diagnosis (CRD)	420:453	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	0	116	theme	carbohydrate	15:26	arg1	interference					40:51	Cross-reactive carbohydrate determinant interference	0:51	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests	0:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	4	117	from	prevalence	845:854	arg1	platform					924:931	a commonly-used in vitro sIgE automated platform	884:931	a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components	884:1016	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	1	118	theme	BACKGROUND	133:142	arg1	structures					190:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures	133:199	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins	133:239	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	1	118	theme	BACKGROUND	133:142	arg1	epitopes					286:293	epitopes	286:293	epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results	286:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	2	119	theme	recombinant	456:466	arg1	components					485:494	recombinant non-glycosylated components	456:494	recombinant non-glycosylated components	456:494	With the introduction of component resolved diagnosis (CRD), recombinant non-glycosylated components have been utilized to mitigate the risk of CCD-specific IgE (sIgE) detection.					
32324770	3	120	theme	phase	663:667	arg1	matrix					669:674	the cellulose solid phase matrix	643:674	the cellulose solid phase matrix used in certain in vitro diagnostic assays	643:717	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	4	121	dep	in	900:901	arg1	vitro					903:907	vitro	903:907	vitro	903:907	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	1	122	theme	obscure	343:349	arg1	results					386:392	obscure in vitro diagnostic (IVD) serology results	343:392	obscure in vitro diagnostic (IVD) serology results	343:392	BACKGROUND Cross-reactive carbohydrate determinant (CCD) structures found in plant and insect glycoproteins are commonly recognized by IgE antibodies as epitopes that can lead to extensive cross-reactivity and obscure in vitro diagnostic (IVD) serology results.					
32324770	3	123	theme	cellulose	647:655	arg1	matrix					669:674	the cellulose solid phase matrix	643:674	the cellulose solid phase matrix used in certain in vitro diagnostic assays	643:717	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	0	124	theme	allergy	76:82	arg1	tests					84:88	cellulose-based IgE allergy tests	56:88	cellulose-based IgE allergy tests	56:88	Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components.					
32324770	5	125	theme	MUXF3	1198:1202	arg1	component					1217:1225	the MUXF3 carbohydrate component	1194:1225	the MUXF3 carbohydrate component	1194:1225	METHODS Sera from patients sensitized to peanut, silver birch, and/or timothy grass were analyzed for CCD-sIgE reactivity on ImmunoCAP/Phadia and NOVEOS autoanalyzers against the MUXF3 carbohydrate component.					
32324770	3	126	theme	certain	684:690	arg1	assays					712:717	certain in vitro diagnostic assays	684:717	certain in vitro diagnostic assays	684:717	However, a recent study has shown that CCD-sIgE may bind directly to the cellulose solid phase matrix used in certain in vitro diagnostic assays, eliminating the advantage of CRD over traditional extract-based testing.					
32324770	9	127	theme	CCD-sIgE-positive	1656:1672	arg1	results					1691:1697	CCD-sIgE-positive (false positive) results	1656:1697	CCD-sIgE-positive (false positive) results on ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS	1656:1778	Of those positives, 17% resulted in CCD-sIgE-positive (false positive) results on ImmunoCAP using non-glycosylated allergosorbents that were negative on NOVEOS.					
32324770	14	128	theme	NOVEOS	2566:2571	arg1	assay					2573:2577	the NOVEOS assay	2562:2577	the NOVEOS assay	2562:2577	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	11	129	theme	low-positive	1958:1969	arg1	kUA/L					2001:2005	0.35 kUA/L- 3.00 kUA/L	1984:2005	0.35 kUA/L- 3.00 kUA/L	1984:2005	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	11	129	theme	low-positive	1958:1969	arg1	results					1975:1981	low-positive IgE results	1958:1981	low-positive IgE results (0.35 kUA/L- 3.00 kUA/L)	1958:2006	The incidence of CCD interference was predominantly delimited to low-positive IgE results (0.35 kUA/L- 3.00 kUA/L).					
32324770	4	130	theme	study	809:813	arg1	aim					797:799	The aim	793:799	The aim of this study	793:813	The aim of this study is to further investigate the prevalence of CCD-sIgE interference on a commonly-used in vitro sIgE automated platform which employs a cellulose-based matrix to immobilize CCD-free recombinant components.					
32324770	14	131	theme	containing	2478:2487	arg1	sera					2489:2492	positive CCD-sIgE containing sera	2460:2492	positive CCD-sIgE containing sera	2460:2492	The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIgE assay, such as the NOVEOS assay.					
32324770	13	132	theme	cellulose	2268:2276	arg1	matrices					2278:2285	cellulose matrices	2268:2285	cellulose matrices	2268:2285	Even the use of non-glycosylated recombinant allergenic components coupled to cellulose matrices do not reduce their risk of detection.					
32464211	3	0	theme	model	627:631	arg1	simulation					633:642	an empirical Box-Behnken model simulation	602:642	an empirical Box-Behnken model simulation	602:642	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	1	1	theme	hydrolysable	235:246	arg1	extract					260:266	the hydrolysable tannin-rich extract	231:266	the hydrolysable tannin-rich extract obtained from fibrous chestnut wood	231:302	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	6	2	theme	properties	1547:1556	arg1	material					1507:1514	the final applicable material	1486:1514	the final applicable material of adequate physico-mechanical properties	1486:1556	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	3	3	theme	independent	660:670	arg1	variables					672:680	three independent variables	654:680	three independent variables: the concentrations of chitosan (1.5-2.0% (w/v))	654:729	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	7	4	theme	absolute	1741:1748	arg1	error					1759:1763	the computed maximal absolute residual error	1720:1763	the computed maximal absolute residual error up to 22.2%	1720:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	3	5	dep	determined	832:841	arg1	%					823:823	30.0-90.0%	814:823	30.0-90.0% (w/w)	814:829	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	5	dep	determined	832:841	arg1	w/w					826:828	w/w	826:828	w/w	826:828	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	7	6	theme	computed	1724:1731	arg1	error					1759:1763	the computed maximal absolute residual error	1720:1763	the computed maximal absolute residual error up to 22.2%	1720:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	6	7	theme	mgGAE	1613:1617	arg1	gfilm-1					1619:1625	TPC = 19.4 mgGAE gfilm-1	1602:1625	TPC = 19.4 mgGAE gfilm-1	1602:1625	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	2	8	theme	content	491:497	arg1	terms					382:386	terms	382:386	terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	382:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	4	9	theme	interaction	931:941	arg1	sets					949:952	two-factor interaction (TPC) sets	920:952	two-factor interaction (TPC) sets	920:952	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	3	10	dep	glycerol	804:811	arg1	determined					832:841	determined	832:841	determined per mass of polysaccharide	832:868	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	7	11	theme	specific	1697:1704	arg1	accuracy					1706:1713	a sufficient specific accuracy	1684:1713	a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%	1684:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	6	12	dep	%	1424:1424	arg1	w/v					1427:1429	w/v	1427:1429	w/v	1427:1429	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	2	13	theme	total	476:480	arg1	TPC					500:502	TPC	500:502	TPC	500:502	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	13	theme	total	476:480	arg1	content					491:497	total phenolic content	476:497	total phenolic content (TPC)	476:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	6	14	theme	chestnut	1432:1439	arg1	extract					1441:1447	0.97% (w/v) chestnut extract	1420:1447	0.97% (w/v) chestnut extract	1420:1447	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	4	15	theme	TPC	944:946	arg1	sets					949:952	two-factor interaction (TPC) sets	920:952	two-factor interaction (TPC) sets	920:952	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	0	16	theme	chitosan-based	118:131	arg1	film					133:136	the chitosan-based film	114:136	the chitosan-based film by response surface methodology (RSM) modelling	114:184	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	6	17	theme	fractions	1384:1392	arg1	formula					1356:1362	The component relationship formula	1329:1362	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol)	1329:1476	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	1	18	theme	chitosan-based	197:210	arg1	film					212:215	An active chitosan-based film	187:215	An active chitosan-based film	187:215	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	1	18	theme	chitosan-based	197:210	arg1	Mill					321:324	Castanea sativa Mill.	305:325	Castanea sativa Mill.	305:325	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	3	19	theme	chitosan	705:712	arg1	concentrations					687:700	the concentrations	683:700	three independent variables: the concentrations of chitosan (1.5-2.0% (w/v))	654:729	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	20	theme	optimal	510:516	arg1	formulation					526:536	The optimal product formulation	506:536	The optimal product formulation for a homogeneous film-forming solution	506:576	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	6	21	theme	chemical	1367:1374	arg1	fractions					1384:1392	chemical mixture fractions	1367:1392	chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol)	1367:1476	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	7	22	dep	error	1759:1763	arg1	%					1775:1775	%	1775:1775	%	1775:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	6	23	theme	%	1424:1424	arg1	extract					1441:1447	0.97% (w/v) chestnut extract	1420:1447	0.97% (w/v) chestnut extract	1420:1447	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	0	24	theme	surface	150:156	arg1	RSM					171:173	RSM	171:173	RSM	171:173	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	0	24	theme	surface	150:156	arg1	methodology					158:168	response surface methodology	141:168	response surface methodology (RSM) modelling	141:184	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	3	25	theme	film-forming	556:567	arg1	solution					569:576	a homogeneous film-forming solution	542:576	a homogeneous film-forming solution	542:576	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	6	26	theme	component	1333:1341	arg1	formula					1356:1362	The component relationship formula	1329:1362	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol)	1329:1476	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	2	27	theme	elongation	446:455	arg1	terms					382:386	terms	382:386	terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	382:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	3	28	theme	powder-form	742:752	arg1	extract					763:769	powder-form chestnut extract	742:769	powder-form chestnut extract (0.5-1.0% (w/v))	742:786	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	29	dep	extract	763:769	arg1	w/v					782:784	w/v	782:784	w/v	782:784	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	29	dep	extract	763:769	arg1	%					779:779	0.5-1.0%	772:779	0.5-1.0% (w/v)	772:785	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	6	30	dep	fractions	1384:1392	arg1	%					1399:1399	1.93%	1395:1399	1.93% (w/v) of chitosan	1395:1417	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	30	dep	fractions	1384:1392	arg1	glycerol					1468:1475	glycerol	1468:1475	glycerol	1468:1475	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	30	dep	fractions	1384:1392	arg1	w/v					1402:1404	w/v	1402:1404	w/v	1402:1404	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	30	dep	fractions	1384:1392	arg1	%					1457:1457	30.0%	1453:1457	30.0% (w/w) of glycerol	1453:1475	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	30	dep	fractions	1384:1392	arg1	chitosan					1410:1417	chitosan	1410:1417	chitosan	1410:1417	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	30	dep	fractions	1384:1392	arg1	w/w					1460:1462	w/w	1460:1462	w/w	1460:1462	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	30	dep	fractions	1384:1392	arg1	extract					1441:1447	0.97% (w/v) chestnut extract	1420:1447	0.97% (w/v) chestnut extract	1420:1447	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	5	31	theme	extract	1267:1273	arg1	variation					1241:1249	the variation	1237:1249	the variation of the chestnut extract	1237:1273	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	0	32	theme	film-forming	75:86	arg1	solution					88:95	the film-forming solution	71:95	the film-forming solution	71:95	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	8	33	theme	Herein-proposed	1778:1792	arg1	methodology					1801:1811	Herein-proposed design methodology	1778:1811	Herein-proposed design methodology	1778:1811	Herein-proposed design methodology can thus be translated to smart packaging fabrication generally.					
32464211	2	34	theme	tensile	423:429	arg1	TS					441:442	TS	441:442	TS	441:442	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	34	theme	tensile	423:429	arg1	strength					431:438	tensile strength	423:438	tensile strength (TS)	423:443	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	7	35	theme	Further	1629:1635	arg1	validation					1649:1658	Further statistical validation	1629:1658	Further statistical validation of the concept	1629:1673	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	4	36	theme	characteristic	1012:1025	arg1	0.969 < R2 < 0.992					1046:1063	0.969 < R2 < 0.992	1046:1063	0.969 < R2 < 0.992	1046:1063	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	4	36	theme	characteristic	1012:1025	arg1	data					1040:1043	characteristic experimental data	1012:1043	characteristic experimental data (0.969 < R2 < 0.992)	1012:1064	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	5	37	theme	system	1116:1121	arg1	parameters					1123:1132	all system parameters	1112:1132	all system parameters	1112:1132	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	0	38	theme	active	15:20	arg1	packaging					27:35	active food packaging	15:35	active food packaging	15:35	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	1	39	theme	fibrous	282:288	arg1	wood					299:302	fibrous chestnut wood	282:302	fibrous chestnut wood	282:302	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	6	40	dep	yielded	1478:1484	arg1	TS = 16.7 MPa					1571:1583	TS = 16.7 MPa	1571:1583	TS = 16.7 MPa	1571:1583	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	40	dep	yielded	1478:1484	arg1	gfilm-1					1619:1625	TPC = 19.4 mgGAE gfilm-1	1602:1625	TPC = 19.4 mgGAE gfilm-1	1602:1625	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	40	dep	yielded	1478:1484	arg1	%					1595:1595	EB = 10.4%	1586:1595	EB = 10.4%	1586:1595	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	40	dep	yielded	1478:1484	arg1	%					1568:1568	MC = 17.0%	1559:1568	MC = 17.0%	1559:1568	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	41	theme	applicable	1496:1505	arg1	material					1507:1514	the final applicable material	1486:1514	the final applicable material of adequate physico-mechanical properties	1486:1556	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	2	42	theme	moisture	400:407	arg1	MC					418:419	MC	418:419	MC	418:419	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	42	theme	moisture	400:407	arg1	content					409:415	measured moisture content	391:415	measured moisture content (MC)	391:420	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	0	43	theme	packaging	27:35	arg1	Formulation					0:10	Formulation	0:10	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.	0:185	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	5	44	theme	continuous	1187:1196	arg1	role					1198:1201	a vital continuous role	1179:1201	a vital continuous role	1179:1201	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	1	45	theme	Castanea	305:312	arg1	Mill					321:324	Castanea sativa Mill.	305:325	Castanea sativa Mill.	305:325	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	1	45	theme	Castanea	305:312	arg1	film					212:215	An active chitosan-based film	187:215	An active chitosan-based film	187:215	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	8	46	theme	smart	1839:1843	arg1	fabrication					1855:1865	smart packaging fabrication	1839:1865	smart packaging fabrication	1839:1865	Herein-proposed design methodology can thus be translated to smart packaging fabrication generally.					
32464211	5	47	theme	connected	1294:1302	arg1	change					1304:1309	an expected connected change	1282:1309	an expected connected change	1282:1309	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	3	48	theme	Box-Behnken	615:625	arg1	simulation					633:642	an empirical Box-Behnken model simulation	602:642	an empirical Box-Behnken model simulation	602:642	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	49	theme	polysaccharide	855:868	arg1	mass					847:850	mass	847:850	mass of polysaccharide	847:868	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	4	50	theme	two-factor	920:929	arg1	sets					949:952	two-factor interaction (TPC) sets	920:952	two-factor interaction (TPC) sets	920:952	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	4	51	dep	significant	971:981	arg1	p < 0.05					984:991	p < 0.05	984:991	p < 0.05	984:991	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	5	52	theme	polyol	1165:1170	arg1	level					1156:1160	the level	1152:1160	the level of polyol	1152:1170	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	2	53	theme	engineering	354:364	arg1	optimization					366:377	a simultaneous engineering optimization	339:377	a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	339:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	0	54	dep	Formulation	0:10	arg1	Linking					48:54	Linking	48:54	Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling	48:184	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	1	55	theme	tannin-rich	248:258	arg1	extract					260:266	the hydrolysable tannin-rich extract	231:266	the hydrolysable tannin-rich extract obtained from fibrous chestnut wood	231:302	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	6	56	theme	physico-mechanical	1528:1545	arg1	properties					1547:1556	adequate physico-mechanical properties	1519:1556	adequate physico-mechanical properties	1519:1556	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	4	57	dep	quadratic	894:902	arg1	EB					911:912	EB	911:912	EB	911:912	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	4	57	dep	quadratic	894:902	arg1	TS					905:906	TS	905:906	TS	905:906	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	7	58	theme	residual	1750:1757	arg1	error					1759:1763	the computed maximal absolute residual error	1720:1763	the computed maximal absolute residual error up to 22.2%	1720:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	7	59	theme	maximal	1733:1739	arg1	error					1759:1763	the computed maximal absolute residual error	1720:1763	the computed maximal absolute residual error up to 22.2%	1720:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	6	60	theme	TPC = 19.4	1602:1611	arg1	mgGAE					1613:1617	mgGAE	1613:1617	mgGAE	1613:1617	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	%					1399:1399	1.93%	1395:1399	1.93% (w/v) of chitosan	1395:1417	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	glycerol					1468:1475	glycerol	1468:1475	glycerol	1468:1475	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	w/v					1402:1404	w/v	1402:1404	w/v	1402:1404	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	%					1457:1457	30.0%	1453:1457	30.0% (w/w) of glycerol	1453:1475	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	chitosan					1410:1417	chitosan	1410:1417	chitosan	1410:1417	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	w/w					1460:1462	w/w	1460:1462	w/w	1460:1462	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	61	theme	chitosan	1410:1417	arg1	extract					1441:1447	0.97% (w/v) chestnut extract	1420:1447	0.97% (w/v) chestnut extract	1420:1447	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	2	62	theme	phenolic	482:489	arg1	TPC					500:502	TPC	500:502	TPC	500:502	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	62	theme	phenolic	482:489	arg1	content					491:497	total phenolic content	476:497	total phenolic content (TPC)	476:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	1	63	dep	blended	218:224	arg1	Mill					321:324	Castanea sativa Mill.	305:325	Castanea sativa Mill.	305:325	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	1	63	dep	blended	218:224	arg1	film					212:215	An active chitosan-based film	187:215	An active chitosan-based film	187:215	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	0	64	theme	film	133:136	arg1	properties					100:109	properties	100:109	properties of the chitosan-based film by response surface methodology (RSM) modelling	100:184	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	3	65	theme	product	518:524	arg1	formulation					526:536	The optimal product formulation	506:536	The optimal product formulation for a homogeneous film-forming solution	506:576	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	1	66	theme	active	190:195	arg1	film					212:215	An active chitosan-based film	187:215	An active chitosan-based film	187:215	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	1	66	theme	active	190:195	arg1	Mill					321:324	Castanea sativa Mill.	305:325	Castanea sativa Mill.	305:325	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	2	67	from	break	460:464	arg1	terms					382:386	terms	382:386	terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	382:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	EB					467:468	EB	467:468	EB	467:468	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	MC					418:419	MC	418:419	MC	418:419	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	TPC					500:502	TPC	500:502	TPC	500:502	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	elongation					446:455	elongation	446:455	elongation at break (EB)	446:469	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	content					409:415	measured moisture content	391:415	measured moisture content (MC)	391:420	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	strength					431:438	tensile strength	423:438	tensile strength (TS)	423:443	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	content					491:497	total phenolic content	476:497	total phenolic content (TPC)	476:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	67	from	break	460:464	arg1	TS					441:442	TS	441:442	TS	441:442	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	1	68	theme	sativa	314:319	arg1	Mill					321:324	Castanea sativa Mill.	305:325	Castanea sativa Mill.	305:325	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	1	68	theme	sativa	314:319	arg1	film					212:215	An active chitosan-based film	187:215	An active chitosan-based film	187:215	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	0	69	theme	response	141:148	arg1	RSM					171:173	RSM	171:173	RSM	171:173	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	0	69	theme	response	141:148	arg1	methodology					158:168	response surface methodology	141:168	response surface methodology (RSM) modelling	141:184	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	6	70	theme	mixture	1376:1382	arg1	fractions					1384:1392	chemical mixture fractions	1367:1392	chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol)	1367:1476	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	7	71	theme	sufficient	1686:1695	arg1	accuracy					1706:1713	a sufficient specific accuracy	1684:1713	a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%	1684:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	0	72	theme	methodology	158:168	arg1	modelling					176:184	response surface methodology (RSM) modelling	141:184	response surface methodology (RSM) modelling	141:184	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	3	73	theme	homogeneous	544:554	arg1	solution					569:576	a homogeneous film-forming solution	542:576	a homogeneous film-forming solution	542:576	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	6	74	theme	0.97	1420:1423	arg1	%					1424:1424	%	1424:1424	%	1424:1424	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	75	theme	relationship	1343:1354	arg1	formula					1356:1362	The component relationship formula	1329:1362	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol)	1329:1476	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	%					1399:1399	1.93%	1395:1399	1.93% (w/v) of chitosan	1395:1417	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	glycerol					1468:1475	glycerol	1468:1475	glycerol	1468:1475	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	w/v					1402:1404	w/v	1402:1404	w/v	1402:1404	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	%					1457:1457	30.0%	1453:1457	30.0% (w/w) of glycerol	1453:1475	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	chitosan					1410:1417	chitosan	1410:1417	chitosan	1410:1417	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	w/w					1460:1462	w/w	1460:1462	w/w	1460:1462	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	6	76	theme	glycerol	1468:1475	arg1	extract					1441:1447	0.97% (w/v) chestnut extract	1420:1447	0.97% (w/v) chestnut extract	1420:1447	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	7	77	theme	concept	1667:1673	arg1	validation					1649:1658	Further statistical validation	1629:1658	Further statistical validation of the concept	1629:1673	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	7	78	dep	22.2	1771:1774	arg1	to					1768:1769	to	1768:1769	to	1768:1769	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	5	79	theme	chestnut	1258:1265	arg1	extract					1267:1273	the chestnut extract	1254:1273	the chestnut extract	1254:1273	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	3	80	theme	chestnut	754:761	arg1	extract					763:769	powder-form chestnut extract	742:769	powder-form chestnut extract (0.5-1.0% (w/v))	742:786	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	0	81	theme	solution	88:95	arg1	composition					56:66	composition	56:66	composition of the film-forming solution	56:95	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	7	82	theme	statistical	1637:1647	arg1	validation					1649:1658	Further statistical validation	1629:1658	Further statistical validation of the concept	1629:1673	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	8	83	theme	design	1794:1799	arg1	methodology					1801:1811	Herein-proposed design methodology	1778:1811	Herein-proposed design methodology	1778:1811	Herein-proposed design methodology can thus be translated to smart packaging fabrication generally.					
32464211	7	84	with	accuracy	1706:1713	arg1	error					1759:1763	the computed maximal absolute residual error	1720:1763	the computed maximal absolute residual error up to 22.2%	1720:1775	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	3	85	theme	plasticizer	792:802	arg1	glycerol					804:811	plasticizer glycerol	792:811	plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide)	792:869	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	7	86	dep	%	1775:1775	arg1	22.2					1771:1774	22.2	1771:1774	22.2	1771:1774	Further statistical validation of the concept revealed a sufficient specific accuracy with the computed maximal absolute residual error up to 22.2%.					
32464211	6	87	theme	adequate	1519:1526	arg1	properties					1547:1556	adequate physico-mechanical properties	1519:1556	adequate physico-mechanical properties	1519:1556	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	4	88	theme	experimental	1027:1038	arg1	0.969 < R2 < 0.992					1046:1063	0.969 < R2 < 0.992	1046:1063	0.969 < R2 < 0.992	1046:1063	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	4	88	theme	experimental	1027:1038	arg1	data					1040:1043	characteristic experimental data	1012:1043	characteristic experimental data (0.969 < R2 < 0.992)	1012:1064	Obtained linear (MC), quadratic (TS or EB), and two-factor interaction (TPC) sets were found to be significant (p < 0.05), to fit well with characteristic experimental data (0.969 < R2 < 0.992), and could be considered predictive.					
32464211	0	89	theme	food	22:25	arg1	packaging					27:35	active food packaging	15:35	active food packaging	15:35	Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.					
32464211	2	90	theme	content	409:415	arg1	terms					382:386	terms	382:386	terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	382:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	1	91	theme	chestnut	290:297	arg1	wood					299:302	fibrous chestnut wood	282:302	fibrous chestnut wood	282:302	An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.)					
32464211	6	92	theme	final	1490:1494	arg1	material					1507:1514	the final applicable material	1486:1514	the final applicable material of adequate physico-mechanical properties	1486:1556	The component relationship formula of chemical mixture fractions (1.93% (w/v) of chitosan, 0.97% (w/v) chestnut extract and 30.0% (w/w) of glycerol) yielded the final applicable material of adequate physico-mechanical properties (MC = 17.0%, TS = 16.7 MPa, EB = 10.4%, and TPC = 19.4 mgGAE gfilm-1).					
32464211	5	93	theme	vital	1181:1185	arg1	role					1198:1201	a vital continuous role	1179:1201	a vital continuous role	1179:1201	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	2	94	theme	measured	391:398	arg1	MC					418:419	MC	418:419	MC	418:419	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	94	theme	measured	391:398	arg1	content					409:415	measured moisture content	391:415	measured moisture content (MC)	391:420	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	95	from	optimization	366:377	arg1	terms					382:386	terms	382:386	terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	382:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	8	96	theme	packaging	1845:1853	arg1	fabrication					1855:1865	smart packaging fabrication	1839:1865	smart packaging fabrication	1839:1865	Herein-proposed design methodology can thus be translated to smart packaging fabrication generally.					
32464211	2	97	theme	strength	431:438	arg1	terms					382:386	terms	382:386	terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	382:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	5	98	theme	expected	1285:1292	arg1	change					1304:1309	an expected connected change	1282:1309	an expected connected change	1282:1309	Although all system parameters were influential, the level of polyol played a vital continuous role in defining EB, MC, and TS, while the variation of the chestnut extract caused an expected connected change in affecting TPC.					
32464211	3	99	theme	empirical	605:613	arg1	simulation					633:642	an empirical Box-Behnken model simulation	602:642	an empirical Box-Behnken model simulation	602:642	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	100	dep	variables	672:680	arg1	concentrations					687:700	the concentrations	683:700	three independent variables: the concentrations of chitosan (1.5-2.0% (w/v))	654:729	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	100	dep	variables	672:680	arg1	w/v					725:727	w/v	725:727	w/v	725:727	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	3	100	dep	variables	672:680	arg1	%					722:722	1.5-2.0%	715:722	1.5-2.0% (w/v)	715:728	The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and plasticizer glycerol (30.0-90.0% (w/w); determined per mass of polysaccharide).					
32464211	2	101	from	terms	382:386	arg1	break					460:464	break	460:464	break	460:464	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
32464211	2	102	theme	simultaneous	341:352	arg1	optimization					366:377	a simultaneous engineering optimization	339:377	a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC)	339:503	, underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC).					
33202752	0	0	theme	High-Performance	80:95	arg1	Chromatography					108:121	High-Performance Thin Layer Chromatography	80:121	High-Performance Thin Layer Chromatography	80:121	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	3	1	theme	floral	666:671	arg1	origin					673:678	the floral origin	662:678	the floral origin	662:678	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	1	2	theme	confidence	326:335	arg1	perspective					337:347	a consumer confidence perspective	315:347	a consumer confidence perspective	315:347	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	5	3	theme	sugar	878:882	arg1	profiles					884:891	HPTLC sugar profiles	872:891	HPTLC sugar profiles	872:891	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33202752	2	4	theme	adulterants	471:481	arg1	detection					452:460	detection	452:460	detection	452:460	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	2	4	theme	adulterants	471:481	arg1	authentication					433:446	authentication	433:446	authentication	433:446	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	2	5	from	authentication	433:446	arg1	honey					486:490	honey	486:490	honey	486:490	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	1	6	theme	sugar	161:165	arg1	syrups					167:172	sugar syrups	161:172	sugar syrups	161:172	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	0	7	theme	Layer	102:106	arg1	Chromatography					108:121	High-Performance Thin Layer Chromatography	80:121	High-Performance Thin Layer Chromatography	80:121	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	1	8	theme	consumer	317:324	arg1	perspective					337:347	a consumer confidence perspective	315:347	a consumer confidence perspective	315:347	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	3	9	theme	honeys	537:542	arg1	composition					503:513	The sugar composition	493:513	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum)	493:599	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	5	10	theme	HPTLC	872:876	arg1	profiles					884:891	HPTLC sugar profiles	872:891	HPTLC sugar profiles	872:891	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33202752	1	11	theme	significant	236:246	arg1	impacts					257:263	significant negative impacts	236:263	significant negative impacts	236:263	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	0	12	theme	Thin	97:100	arg1	Chromatography					108:121	High-Performance Thin Layer Chromatography	80:121	High-Performance Thin Layer Chromatography	80:121	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	1	13	dep	both	288:291	arg1	economically					293:304	economically	293:304	economically	293:304	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	7	14	theme	sucrose	1146:1152	arg1	Quantifications					1127:1141	Quantifications	1127:1141	Quantifications of sucrose and maltose	1127:1164	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	7	15	theme	organic	1292:1298	arg1	extracts					1306:1313	the organic honey extracts	1288:1313	the organic honey extracts	1288:1313	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	6	16	theme	fructose	1035:1042	arg1	honey					1006:1010	honey	1006:1010	honey	1006:1010	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	16	theme	fructose	1035:1042	arg1	quantification					1017:1030	the quantification	1013:1030	the quantification of fructose and glucose	1013:1054	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	16	theme	fructose	1035:1042	arg1	ratio					1081:1085	their concentration ratio	1061:1085	their concentration ratio	1061:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	3	17	theme	various	518:524	arg1	honeys					537:542	various Australian honeys	518:542	various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum)	518:599	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	7	18	theme	syrup	1202:1206	arg1	adulterant					1208:1217	syrup adulterant	1202:1217	syrup adulterant	1202:1217	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	1	19	theme	syrups	167:172	arg1	range					152:156	a range	150:156	a range of sugar syrups	150:172	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	20	dep	industry	278:285	arg1	perspective					337:347	a consumer confidence perspective	315:347	a consumer confidence perspective	315:347	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	20	dep	industry	278:285	arg1	both					288:291	both	288:291	both	288:291	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	2	21	from	detection	452:460	arg1	honey					486:490	honey	486:490	honey	486:490	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	3	22	theme	Australian	526:535	arg1	honeys					537:542	various Australian honeys	518:542	various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum)	518:599	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	2	23	theme	High-Performance	374:389	arg1	Chromatography					402:415	High-Performance Thin Layer Chromatography	374:415	High-Performance Thin Layer Chromatography (HPTLC)	374:423	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	2	23	theme	High-Performance	374:389	arg1	HPTLC					418:422	HPTLC	418:422	HPTLC	418:422	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	2	24	dep	authentication	433:446	arg1	the					429:431	the	429:431	the	429:431	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	5	25	theme	organic	914:920	arg1	profiles					930:937	organic extract profiles	914:937	organic extract profiles	914:937	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33202752	4	26	theme	honeys	692:697	arg1	Jarrah					711:716	Jarrah	711:716	Jarrah	711:716	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	26	theme	honeys	692:697	arg1	Manuka					700:705	Manuka	700:705	Manuka	700:705	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	26	theme	honeys	692:697	arg1	Two					681:683	Two	681:683	Two	681:683	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	26	theme	honeys	692:697	arg1	honeys					692:697	the honeys	688:697	the honeys (Manuka and Jarrah)	688:717	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	6	27	theme	As	981:982	arg1	sugars					990:995	As major sugars	981:995	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio	981:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	0	28	theme	Sugar	0:4	arg1	Profiling					6:14	Sugar Profiling	0:14	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.	0:122	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	7	29	theme	honey	1300:1304	arg1	extracts					1306:1313	the organic honey extracts	1288:1313	the organic honey extracts	1288:1313	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	7	30	theme	adulterant	1208:1217	arg1	type					1194:1197	the type	1190:1197	the type of syrup adulterant	1190:1217	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	0	31	theme	Honeys	19:24	arg1	Profiling					6:14	Sugar Profiling	0:14	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.	0:122	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	3	32	theme	Peppermint	575:584	arg1	Jarrah					553:558	Jarrah	553:558	Jarrah	553:558	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	3	32	theme	Peppermint	575:584	arg1	Gum					596:598	Peppermint and White Gum	575:598	Peppermint and White Gum	575:598	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	7	33	used	used	1173:1176	arg2	Quantifications					1127:1141	Quantifications	1127:1141	Quantifications of sucrose and maltose	1127:1164	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	6	34	theme	glucose	1048:1054	arg1	honey					1006:1010	honey	1006:1010	honey	1006:1010	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	34	theme	glucose	1048:1054	arg1	quantification					1017:1030	the quantification	1013:1030	the quantification of fructose and glucose	1013:1054	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	34	theme	glucose	1048:1054	arg1	ratio					1081:1085	their concentration ratio	1061:1085	their concentration ratio	1061:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	7	35	theme	HPTLC	1264:1268	arg1	fingerprinting					1270:1283	HPTLC fingerprinting	1264:1283	HPTLC fingerprinting of the organic honey extracts	1264:1313	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	4	36	dep	syrups	779:784	arg1	rice					787:790	rice	787:790	rice	787:790	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	36	dep	syrups	779:784	arg1	corn					793:796	corn	793:796	corn	793:796	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	36	dep	syrups	779:784	arg1	glucose					816:822	glucose	816:822	glucose	816:822	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	36	dep	syrups	779:784	arg1	syrups					779:784	six different sugar syrups	759:784	six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup)	759:839	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	36	dep	syrups	779:784	arg1	golden					799:804	golden	799:804	golden	799:804	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	36	dep	syrups	779:784	arg1	syrup					834:838	maple syrup	828:838	maple syrup	828:838	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	36	dep	syrups	779:784	arg1	treacle					807:813	treacle	807:813	treacle	807:813	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	6	37	theme	concentration	1067:1079	arg1	ratio					1081:1085	their concentration ratio	1061:1085	their concentration ratio	1061:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	3	38	theme	sugar	497:501	arg1	composition					503:513	The sugar composition	493:513	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum)	493:599	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	1	39	theme	bulk	194:197	arg1	volume					199:204	bulk volume	194:204	bulk volume	194:204	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	2	40	theme	sugar	465:469	arg1	adulterants					471:481	sugar adulterants	465:481	sugar adulterants	465:481	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	1	41	theme	honey	272:276	arg1	industry					278:285	the honey industry	268:285	the honey industry	268:285	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	4	42	theme	different	763:771	arg1	rice					787:790	rice	787:790	rice	787:790	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	42	theme	different	763:771	arg1	corn					793:796	corn	793:796	corn	793:796	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	42	theme	different	763:771	arg1	glucose					816:822	glucose	816:822	glucose	816:822	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	42	theme	different	763:771	arg1	syrups					779:784	six different sugar syrups	759:784	six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup)	759:839	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	42	theme	different	763:771	arg1	golden					799:804	golden	799:804	golden	799:804	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	42	theme	different	763:771	arg1	syrup					834:838	maple syrup	828:838	maple syrup	828:838	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	42	theme	different	763:771	arg1	treacle					807:813	treacle	807:813	treacle	807:813	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	2	43	theme	Thin	391:394	arg1	Chromatography					402:415	High-Performance Thin Layer Chromatography	374:415	High-Performance Thin Layer Chromatography (HPTLC)	374:423	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	2	43	theme	Thin	391:394	arg1	HPTLC					418:422	HPTLC	418:422	HPTLC	418:422	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	6	44	located	found	997:1001	arg2	sugars					990:995	As major sugars	981:995	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio	981:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	44	located	found	997:1001	arg1	honey					1006:1010	honey	1006:1010	honey	1006:1010	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	44	located	found	997:1001	arg1	ratio					1081:1085	their concentration ratio	1061:1085	their concentration ratio	1061:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	6	44	located	found	997:1001	arg1	quantification					1017:1030	the quantification	1013:1030	the quantification of fructose and glucose	1013:1054	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	2	45	theme	Layer	396:400	arg1	Chromatography					402:415	High-Performance Thin Layer Chromatography	374:415	High-Performance Thin Layer Chromatography (HPTLC)	374:423	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	2	45	theme	Layer	396:400	arg1	HPTLC					418:422	HPTLC	418:422	HPTLC	418:422	This paper investigates High-Performance Thin Layer Chromatography (HPTLC) for the authentication and detection of sugar adulterants in honey.					
33202752	7	46	with	combination	1247:1257	arg1	fingerprinting					1270:1283	HPTLC fingerprinting	1264:1283	HPTLC fingerprinting of the organic honey extracts	1264:1313	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	1	47	theme	Honey	124:128	arg1	problem					219:225	a common problem	210:225	a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective	210:347	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	47	theme	Honey	124:128	arg1	adulteration					130:141	Honey adulteration	124:141	Honey adulteration	124:141	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	48	dep	significant	236:246	arg1	negative					248:255	negative	248:255	negative	248:255	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	4	49	theme	sugar	773:777	arg1	rice					787:790	rice	787:790	rice	787:790	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	49	theme	sugar	773:777	arg1	corn					793:796	corn	793:796	corn	793:796	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	49	theme	sugar	773:777	arg1	glucose					816:822	glucose	816:822	glucose	816:822	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	49	theme	sugar	773:777	arg1	syrups					779:784	six different sugar syrups	759:784	six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup)	759:839	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	49	theme	sugar	773:777	arg1	golden					799:804	golden	799:804	golden	799:804	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	49	theme	sugar	773:777	arg1	syrup					834:838	maple syrup	828:838	maple syrup	828:838	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	49	theme	sugar	773:777	arg1	treacle					807:813	treacle	807:813	treacle	807:813	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	50	theme	maple	828:832	arg1	syrup					834:838	maple syrup	828:838	maple syrup	828:838	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	50	theme	maple	828:832	arg1	syrups					779:784	six different sugar syrups	759:784	six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup)	759:839	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	3	51	theme	White	590:594	arg1	Jarrah					553:558	Jarrah	553:558	Jarrah	553:558	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	3	51	theme	White	590:594	arg1	Gum					596:598	Peppermint and White Gum	575:598	Peppermint and White Gum	575:598	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	7	52	theme	extracts	1306:1313	arg1	fingerprinting					1270:1283	HPTLC fingerprinting	1264:1283	HPTLC fingerprinting of the organic honey extracts	1264:1313	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	3	53	dep	honeys	537:542	arg1	Karri					568:572	Karri	568:572	Karri	568:572	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	3	53	dep	honeys	537:542	arg1	Jarrah					553:558	Jarrah	553:558	Jarrah	553:558	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	3	53	dep	honeys	537:542	arg1	Gum					596:598	Peppermint and White Gum	575:598	Peppermint and White Gum	575:598	The sugar composition of various Australian honeys (Manuka, Jarrah, Marri, Karri, Peppermint and White Gum) was first determined to illustrate the variance depending on the floral origin.					
33202752	6	54	used	used	1094:1097	arg2	sugars					990:995	As major sugars	981:995	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio	981:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	5	55	theme	sugar	962:966	arg1	adulterants					968:978	the sugar adulterants	958:978	the sugar adulterants	958:978	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33202752	1	56	used	used	177:180	arg2	range					152:156	a range	150:156	a range of sugar syrups	150:172	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	4	57	dep	honeys	692:697	arg1	Jarrah					711:716	Jarrah	711:716	Jarrah	711:716	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	57	dep	honeys	692:697	arg1	Manuka					700:705	Manuka	700:705	Manuka	700:705	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	4	57	dep	honeys	692:697	arg1	honeys					692:697	the honeys	688:697	the honeys (Manuka and Jarrah)	688:717	Two of the honeys (Manuka and Jarrah) were then artificially adulterated with six different sugar syrups (rice, corn, golden, treacle, glucose and maple syrup).					
33202752	0	58	theme	Adulterants	62:72	arg1	Authentication					30:43	Authentication	30:43	Authentication	30:43	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	0	58	theme	Adulterants	62:72	arg1	Detection					49:57	Detection	49:57	Detection	49:57	Sugar Profiling of Honeys for Authentication and Detection of Adulterants Using High-Performance Thin Layer Chromatography.					
33202752	5	59	from	profiles	884:891	arg1	combination					897:907	combination	897:907	combination with organic extract profiles	897:937	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33202752	1	60	contain	has	232:234	arg2	impacts					257:263	significant negative impacts	236:263	significant negative impacts	236:263	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	60	contain	has	232:234	arg1	problem					219:225	a common problem	210:225	a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective	210:347	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	60	contain	has	232:234	arg1	adulteration					130:141	Honey adulteration	124:141	Honey adulteration	124:141	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	6	61	theme	major	984:988	arg1	sugars					990:995	As major sugars	981:995	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio	981:1085	As major sugars found in honey, the quantification of fructose and glucose, and their concentration ratio can be used to authenticate the honeys.					
33202752	1	62	theme	common	212:217	arg1	problem					219:225	a common problem	210:225	a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective	210:347	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	1	62	theme	common	212:217	arg1	adulteration					130:141	Honey adulteration	124:141	Honey adulteration	124:141	Honey adulteration, where a range of sugar syrups is used to increase bulk volume, is a common problem that has significant negative impacts on the honey industry, both economically and from a consumer confidence perspective.					
33202752	5	63	theme	extract	922:928	arg1	profiles					930:937	organic extract profiles	914:937	organic extract profiles	914:937	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33202752	7	64	theme	maltose	1158:1164	arg1	Quantifications					1127:1141	Quantifications	1127:1141	Quantifications of sucrose and maltose	1127:1164	Quantifications of sucrose and maltose can be used to identify the type of syrup adulterant, in particular when used in combination with HPTLC fingerprinting of the organic honey extracts.					
33202752	5	65	with	combination	897:907	arg1	profiles					930:937	organic extract profiles	914:937	organic extract profiles	914:937	The findings demonstrate that HPTLC sugar profiles, in combination with organic extract profiles, can easily detect the sugar adulterants.					
33450339	8	0	theme	future	1381:1386	arg1	production					1399:1408	future industrial production	1381:1408	future industrial production	1381:1408	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	0	1	theme	tensile	78:84	arg1	property					86:93	tensile property	78:93	tensile property	78:93	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	8	2	theme	theoretical	1357:1367	arg1	support					1369:1375	theoretical support	1357:1375	theoretical support for future industrial production	1357:1408	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	8	3	theme	feasible	1267:1274	arg1	method					1276:1281	a simple and feasible method	1254:1281	a simple and feasible method for the preparation of low-cost PBS bio-composite materials	1254:1341	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	4	4	theme	tensile	639:645	arg1	property					647:654	tensile property	639:654	tensile property	639:654	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	0	5	theme	PBS/corn	98:105	arg1	starch					107:112	PBS/corn starch	98:112	PBS/corn starch	98:112	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	0	6	from	Effect	0:5	arg1	structure					64:72	structure	64:72	structure	64:72	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	0	6	from	Effect	0:5	arg1	property					86:93	tensile property	78:93	tensile property	78:93	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	6	7	theme	homogeneous	1060:1070	arg1	phase					1072:1076	homogeneous phase	1060:1076	homogeneous phase	1060:1076	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	4	8	theme	IL	615:616	arg1	effects					604:610	The effects	600:610	The effects of IL on the structure and tensile property of PBS/Starch blends	600:675	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	2	9	theme	pre-plasticized	288:302	arg1	CS					317:318	CS	317:318	CS	317:318	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	2	9	theme	pre-plasticized	288:302	arg1	starch					309:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch	254:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS)	254:319	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	1	10	theme	promising	127:135	arg1	resin					151:155	a promising biodegradable resin	125:155	a promising biodegradable resin	125:155	As a promising biodegradable resin, poly (butylene succinate) (PBS) is often blended with starch to reduce the cost.					
33450339	1	10	theme	promising	127:135	arg1	poly					158:161	poly	158:161	poly (butylene succinate) (PBS)	158:188	As a promising biodegradable resin, poly (butylene succinate) (PBS) is often blended with starch to reduce the cost.					
33450339	5	11	theme	blends	836:841	arg1	crystallinity					808:820	the crystallinity	804:820	the crystallinity of PBS/Starch blends	804:841	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	5	12	theme	PBS	884:886	arg1	matrix					888:893	the PBS matrix	880:893	the PBS matrix	880:893	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	2	13	theme	PBS/corn	353:360	arg1	material					375:382	PBS/corn starch blend material	353:382	PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL)	353:419	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	0	14	theme	starch	107:112	arg1	structure					64:72	structure	64:72	structure	64:72	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	0	14	theme	starch	107:112	arg1	property					86:93	tensile property	78:93	tensile property	78:93	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	5	15	theme	PBS/Starch	825:834	arg1	blends					836:841	PBS/Starch blends	825:841	PBS/Starch blends	825:841	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	4	16	dep	structure	625:633	arg1	the					621:623	the	621:623	the	621:623	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	1	17	theme	butylene	164:171	arg1	succinate					173:181	butylene succinate	164:181	butylene succinate	164:181	As a promising biodegradable resin, poly (butylene succinate) (PBS) is often blended with starch to reduce the cost.					
33450339	1	17	theme	butylene	164:171	arg1	poly					158:161	poly	158:161	poly (butylene succinate) (PBS)	158:188	As a promising biodegradable resin, poly (butylene succinate) (PBS) is often blended with starch to reduce the cost.					
33450339	6	18	theme	sea-island	1035:1044	arg1	structure					1047:1055	"sea-island" structure	1034:1055	"sea-island" structure to homogeneous phase	1034:1076	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	3	19	theme	1-butyl-3-methylimidazolium	500:526	arg1	halide					528:533	1-butyl-3-methylimidazolium halide	500:533	1-butyl-3-methylimidazolium halide with different halogen anion	500:562	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
33450339	7	20	theme	PBS/Starch	1141:1150	arg1	blend					1152:1156	the PBS/Starch blend	1137:1156	the PBS/Starch blend without IL	1137:1167	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	4	21	theme	blends	670:675	arg1	structure					625:633	structure	625:633	structure	625:633	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	4	21	theme	blends	670:675	arg1	property					647:654	tensile property	639:654	tensile property	639:654	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	3	22	theme	halide	528:533	arg1	effects					489:495	the effects	485:495	the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends	485:583	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
33450339	4	23	from	effects	604:610	arg1	structure					625:633	structure	625:633	structure	625:633	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	4	23	from	effects	604:610	arg1	property					647:654	tensile property	639:654	tensile property	639:654	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	6	24	theme	starch	962:967	arg1	compatibilizer					944:957	a compatibilizer	942:957	a compatibilizer of starch and PBS	942:975	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	6	24	theme	starch	962:967	arg1	IL					925:926	IL	925:926	IL	925:926	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	6	24	theme	starch	962:967	arg1	morphology					994:1003	the morphology	990:1003	the morphology of the blends to change from "sea-island" structure to homogeneous phase	990:1076	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	0	25	theme	liquid	16:21	arg1	halide					50:55	ionic liquid 1-buyl-3-methylimidazolium halide	10:55	ionic liquid 1-buyl-3-methylimidazolium halide	10:55	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	6	26	theme	PBS	973:975	arg1	compatibilizer					944:957	a compatibilizer	942:957	a compatibilizer of starch and PBS	942:975	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	6	26	theme	PBS	973:975	arg1	IL					925:926	IL	925:926	IL	925:926	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	6	26	theme	PBS	973:975	arg1	morphology					994:1003	the morphology	990:1003	the morphology of the blends to change from "sea-island" structure to homogeneous phase	990:1076	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	7	27	theme	93	1229:1230	arg1	%					1224:1224	%	1224:1224	%	1224:1224	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	2	28	theme	1-buyl-3-methylimidazolium	254:279	arg1	CS					317:318	CS	317:318	CS	317:318	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	2	28	theme	1-buyl-3-methylimidazolium	254:279	arg1	starch					309:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch	254:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS)	254:319	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	5	29	theme	particles	867:875	arg1	size					852:855	the size	848:855	the size of starch particles in the PBS matrix	848:893	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	2	30	theme	halide	281:286	arg1	CS					317:318	CS	317:318	CS	317:318	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	2	30	theme	halide	281:286	arg1	starch					309:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch	254:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS)	254:319	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	0	31	dep	structure	64:72	arg1	the					60:62	the	60:62	the	60:62	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	0	32	theme	halide	50:55	arg1	Effect					0:5	Effect	0:5	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch	0:112	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	8	33	theme	bio-composite	1319:1331	arg1	materials					1333:1341	low-cost PBS bio-composite materials	1306:1341	low-cost PBS bio-composite materials	1306:1341	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	3	34	theme	halogen	550:556	arg1	anion					558:562	different halogen anion	540:562	different halogen anion	540:562	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
33450339	2	35	theme	blend	369:373	arg1	material					375:382	PBS/corn starch blend material	353:382	PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL)	353:419	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	0	36	theme	1-buyl-3-methylimidazolium	23:48	arg1	halide					50:55	ionic liquid 1-buyl-3-methylimidazolium halide	10:55	ionic liquid 1-buyl-3-methylimidazolium halide	10:55	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	5	37	theme	Test	738:741	arg1	results					743:749	Test results	738:749	Test results	738:749	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	2	38	theme	starch	362:367	arg1	material					375:382	PBS/corn starch blend material	353:382	PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL)	353:419	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	8	39	theme	industrial	1388:1397	arg1	production					1399:1408	future industrial production	1381:1408	future industrial production	1381:1408	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	4	40	theme	PBS/Starch	659:668	arg1	blends					670:675	PBS/Starch blends	659:675	PBS/Starch blends	659:675	The effects of IL on the structure and tensile property of PBS/Starch blends were evaluated by FTIR, SEM, DSC, TGA and XRD, respectively.					
33450339	8	41	theme	PBS	1315:1317	arg1	materials					1333:1341	low-cost PBS bio-composite materials	1306:1341	low-cost PBS bio-composite materials	1306:1341	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	3	42	theme	different	540:548	arg1	anion					558:562	different halogen anion	540:562	different halogen anion	540:562	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
33450339	7	43	theme	PBS/CS-IL	1197:1205	arg1	elongation					1174:1183	the elongation	1170:1183	the elongation at break of PBS/CS-IL	1170:1205	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	6	44	theme	blends	1012:1017	arg1	compatibilizer					944:957	a compatibilizer	942:957	a compatibilizer of starch and PBS	942:975	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	6	44	theme	blends	1012:1017	arg1	IL					925:926	IL	925:926	IL	925:926	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	6	44	theme	blends	1012:1017	arg1	morphology					994:1003	the morphology	990:1003	the morphology of the blends to change from "sea-island" structure to homogeneous phase	990:1076	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	3	45	from	effects	489:495	arg1	blends					578:583	PBS/Starch blends	567:583	PBS/Starch blends	567:583	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
33450339	3	46	theme	PBS/Starch	567:576	arg1	blends					578:583	PBS/Starch blends	567:583	PBS/Starch blends	567:583	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
33450339	8	47	theme	low-cost	1306:1313	arg1	materials					1333:1341	low-cost PBS bio-composite materials	1306:1341	low-cost PBS bio-composite materials	1306:1341	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	7	48	theme	22	1222:1223	arg1	%					1224:1224	%	1224:1224	%	1224:1224	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	5	49	theme	starch	860:865	arg1	particles					867:875	starch particles	860:875	starch particles	860:875	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	6	50	theme	"	1045:1045	arg1	structure					1047:1055	"sea-island" structure	1034:1055	"sea-island" structure to homogeneous phase	1034:1076	IL also acted as a compatibilizer of starch and PBS, and induced the morphology of the blends to change from "sea-island" structure to homogeneous phase.					
33450339	8	51	theme	materials	1333:1341	arg1	preparation					1291:1301	the preparation	1287:1301	the preparation of low-cost PBS bio-composite materials	1287:1341	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	8	52	theme	simple	1256:1261	arg1	method					1276:1281	a simple and feasible method	1254:1281	a simple and feasible method for the preparation of low-cost PBS bio-composite materials	1254:1341	This study provided a simple and feasible method for the preparation of low-cost PBS bio-composite materials, and provided theoretical support for future industrial production.					
33450339	2	53	theme	corn	304:307	arg1	CS					317:318	CS	317:318	CS	317:318	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	2	53	theme	corn	304:307	arg1	starch					309:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch	254:314	1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS)	254:319	In this paper, 1-buyl-3-methylimidazolium halide pre-plasticized corn starch (CS) was blended with PBS to prepare PBS/corn starch blend material modified by ionic liquid (PBS/CS-IL).					
33450339	5	54	from	size	852:855	arg1	matrix					888:893	the PBS matrix	880:893	the PBS matrix	880:893	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	7	55	theme	tensile	1098:1104	arg1	test					1106:1109	the tensile test	1094:1109	the tensile test	1094:1109	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	7	56	dep	%	1224:1224	arg1	to					1226:1227	to	1226:1227	to	1226:1227	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	7	57	from	break	1188:1192	arg1	elongation					1174:1183	the elongation	1170:1183	the elongation at break of PBS/CS-IL	1170:1205	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	1	58	theme	biodegradable	137:149	arg1	resin					151:155	a promising biodegradable resin	125:155	a promising biodegradable resin	125:155	As a promising biodegradable resin, poly (butylene succinate) (PBS) is often blended with starch to reduce the cost.					
33450339	1	58	theme	biodegradable	137:149	arg1	poly					158:161	poly	158:161	poly (butylene succinate) (PBS)	158:188	As a promising biodegradable resin, poly (butylene succinate) (PBS) is often blended with starch to reduce the cost.					
33450339	5	59	theme	IL	779:780	arg1	addition					767:774	the addition	763:774	the addition of IL	763:780	Test results showed that the addition of IL significantly reduced the crystallinity of PBS/Starch blends, and the size of starch particles in the PBS matrix was also effectively reduced.					
33450339	7	60	theme	test	1106:1109	arg1	results					1083:1089	The results	1079:1089	The results of the tensile test	1079:1109	The results of the tensile test showed that compared with the PBS/Starch blend without IL, the elongation at break of PBS/CS-IL increased from 22% to 93%.					
33450339	0	61	theme	ionic	10:14	arg1	halide					50:55	ionic liquid 1-buyl-3-methylimidazolium halide	10:55	ionic liquid 1-buyl-3-methylimidazolium halide	10:55	Effect of ionic liquid 1-buyl-3-methylimidazolium halide on the structure and tensile property of PBS/corn starch blends.					
33450339	3	62	with	halide	528:533	arg1	anion					558:562	different halogen anion	540:562	different halogen anion	540:562	Ionic liquid (IL) acted as plasticizer and compatibilizer, and the effects of 1-butyl-3-methylimidazolium halide with different halogen anion on PBS/Starch blends were explored.					
32998015	8	0	theme	highest	1096:1102	arg1	stability					1114:1122	oxidative stability	1104:1122	oxidative stability	1104:1122	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	9	1	theme	film	1233:1236	arg1	color					1238:1242	Pec/Clay/CCE/βC film color	1217:1242	Pec/Clay/CCE/βC film color	1217:1242	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	5	2	theme	%	688:688	arg1	%					696:696	So the Pec/Clay/CCE0.5%/BC0.03% film	666:701	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film	666:721	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film was used for packaging of local butter.					
32998015	8	3	theme	active	1065:1070	arg1	butter					1081:1086	active packaged butter	1065:1086	active packaged butter	1065:1086	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	8	4	theme	oxidative	1104:1112	arg1	stability					1114:1122	oxidative stability	1104:1122	oxidative stability	1104:1122	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	4	5	theme	maximum	541:547	arg1	oil					559:561	maximum essential oil	541:561	maximum essential oil	541:561	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	7	6	theme	Pec/Clay/CCE/βC	931:945	arg1	film					947:950	Pec/Clay/CCE/βC film	931:950	Pec/Clay/CCE/βC film	931:950	Results showed that Pec/Clay/CCE/βC film was effective against Bacillus cereus (B. cereus) more than Escherichia coli (E. coli).					
32998015	1	7	theme	montmorillonite	170:184	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	8	8	theme	microbial	1132:1140	arg1	load					1142:1145	lowest microbial load	1125:1145	lowest microbial load	1125:1145	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	6	9	theme	L	823:823	arg1	*					824:824	L*	823:824	L*	823:824	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	8	10	contain	had	1088:1090	arg2	change					1168:1173	the least color change	1152:1173	the least color change	1152:1173	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	8	10	contain	had	1088:1090	arg1	butter					1081:1086	active packaged butter	1065:1086	active packaged butter	1065:1086	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	8	10	contain	had	1088:1090	arg2	stability					1114:1122	oxidative stability	1104:1122	oxidative stability	1104:1122	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	8	10	contain	had	1088:1090	arg2	load					1142:1145	lowest microbial load	1125:1145	lowest microbial load	1125:1145	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	0	11	theme	pectin/nanoclay/Carum	80:100	arg1	oils/β-carotene					121:135	pectin/nanoclay/Carum copticum essential oils/β-carotene	80:135	pectin/nanoclay/Carum copticum essential oils/β-carotene	80:135	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	6	12	theme	active	862:867	arg1	Pec/Clay/CCE/βC					875:889	Pec/Clay/CCE/βC	875:889	Pec/Clay/CCE/βC	875:889	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	6	12	theme	active	862:867	arg1	film					869:872	active film	862:872	active film (Pec/Clay/CCE/βC)	862:890	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	7	13	dep	cereus	983:988	arg1	cereus					994:999	B. cereus	991:999	B. cereus	991:999	Results showed that Pec/Clay/CCE/βC film was effective against Bacillus cereus (B. cereus) more than Escherichia coli (E. coli).					
32998015	4	14	theme	antioxidant	618:628	arg1	activity					630:637	antioxidant activity	618:637	antioxidant activity	618:637	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	5	15	theme	butter	755:760	arg1	packaging					736:744	packaging	736:744	packaging of local butter	736:760	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film was used for packaging of local butter.					
32998015	8	16	theme	lowest	1125:1130	arg1	load					1142:1145	lowest microbial load	1125:1145	lowest microbial load	1125:1145	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	9	17	theme	time	1391:1394	arg1	oxidation					1356:1364	the oxidation	1352:1364	the oxidation of butter and expiration time of butter	1352:1404	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	18	theme	butter	1369:1374	arg1	oxidation					1356:1364	the oxidation	1352:1364	the oxidation of butter and expiration time of butter	1352:1404	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	0	19	theme	essential	111:119	arg1	oils/β-carotene					121:135	pectin/nanoclay/Carum copticum essential oils/β-carotene	80:135	pectin/nanoclay/Carum copticum essential oils/β-carotene	80:135	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	8	20	theme	least	1156:1160	arg1	change					1168:1173	the least color change	1152:1173	the least color change	1152:1173	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	6	21	theme	color	805:809	arg1	properties					811:820	color properties	805:820	color properties	805:820	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	2	22	theme	β-Carotene	289:298	arg1	effect					279:284	The effect	275:284	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film	275:441	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	1	23	theme	/Carum	186:191	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	9	24	used	used	1310:1313	arg2	change					1299:1304	this color change	1288:1304	this color change	1288:1304	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	24	used	used	1310:1313	arg2	indicator					1332:1340	a smart color indicator	1318:1340	a smart color indicator	1318:1340	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	6	25	theme	butter	841:846	arg1	stability					773:781	Oxidative stability	763:781	Oxidative stability	763:781	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	6	25	theme	butter	841:846	arg1	count					794:798	microbial count	784:798	microbial count	784:798	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	6	25	theme	butter	841:846	arg1	properties					811:820	color properties	805:820	color properties	805:820	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	2	26	theme	Essential	324:332	arg1	CCE					340:342	CCE	340:342	CCE	340:342	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	26	theme	Essential	324:332	arg1	oils					334:337	Carum copticum Essential oils	309:337	Carum copticum Essential oils (CCE) concentration	309:357	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	1	27	theme	copticum	193:200	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	0	28	theme	Bacterial	0:8	arg1	control					24:30	Bacterial and oxidative control	0:30	Bacterial and oxidative control of local butter with smart/active film	0:69	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	9	29	theme	light	1271:1275	arg1	yellow					1277:1282	light yellow	1271:1282	light yellow	1271:1282	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	5	30	theme	Pec/Clay/CCE0.5	673:687	arg1	%					696:696	So the Pec/Clay/CCE0.5%/BC0.03% film	666:701	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film	666:721	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film was used for packaging of local butter.					
32998015	1	31	theme	Essential	202:210	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	8	32	theme	color	1162:1166	arg1	change					1168:1173	the least color change	1152:1173	the least color change	1152:1173	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	2	33	theme	Carum	309:313	arg1	CCE					340:342	CCE	340:342	CCE	340:342	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	33	theme	Carum	309:313	arg1	oils					334:337	Carum copticum Essential oils	309:337	Carum copticum Essential oils (CCE) concentration	309:357	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	1	34	theme	oils/β-Carotene	212:226	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	0	35	theme	oxidative	14:22	arg1	control					24:30	Bacterial and oxidative control	0:30	Bacterial and oxidative control of local butter with smart/active film	0:69	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	4	36	theme	antibacterial	594:606	arg1	activity					608:615	antibacterial activity	594:615	antibacterial activity	594:615	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	4	37	contain	containing	530:539	arg1	films					524:528	The films	520:528	The films containing maximum essential oil and β-carotene	520:576	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	4	37	contain	containing	530:539	arg2	β-carotene					567:576	β-carotene	567:576	β-carotene	567:576	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	4	37	contain	containing	530:539	arg2	oil					559:561	maximum essential oil	541:561	maximum essential oil	541:561	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	2	38	theme	concentration	345:357	arg1	effect					279:284	The effect	275:284	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film	275:441	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	39	theme	film	438:441	arg1	activity					378:385	antioxidant activity	366:385	antioxidant activity	366:385	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	39	theme	film	438:441	arg1	properties					408:417	color/mechanical properties	391:417	color/mechanical properties	391:417	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	0	40	theme	local	35:39	arg1	butter					41:46	local butter	35:46	local butter with smart/active film	35:69	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	4	41	theme	best	589:592	arg1	activity					608:615	antibacterial activity	594:615	antibacterial activity	594:615	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	1	42	theme	Pec/Clay/CCE/βC	229:243	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	2	43	from	effect	279:284	arg1	activity					378:385	antioxidant activity	366:385	antioxidant activity	366:385	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	43	from	effect	279:284	arg1	properties					408:417	color/mechanical properties	391:417	color/mechanical properties	391:417	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	44	theme	Pec/Clay/CCE/βC	422:436	arg1	film					438:441	Pec/Clay/CCE/βC film	422:441	Pec/Clay/CCE/βC film	422:441	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	9	45	theme	color	1293:1297	arg1	change					1299:1304	this color change	1288:1304	this color change	1288:1304	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	45	theme	color	1293:1297	arg1	indicator					1332:1340	a smart color indicator	1318:1340	a smart color indicator	1318:1340	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	6	46	theme	microbial	784:792	arg1	count					794:798	microbial count	784:798	microbial count	784:798	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	3	47	theme	antibacterial	475:487	arg1	activity					489:496	antibacterial activity	475:496	antibacterial activity	475:496	The morphology and antibacterial activity of film were studied.					
32998015	5	48	used	used	727:730	arg2	%					696:696	So the Pec/Clay/CCE0.5%/BC0.03% film	666:701	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film	666:721	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film was used for packaging of local butter.					
32998015	4	49	theme	essential	549:557	arg1	oil					559:561	maximum essential oil	541:561	maximum essential oil	541:561	The films containing maximum essential oil and β-carotene showed the best antibacterial activity, antioxidant activity, flexibility and firmness.					
32998015	6	50	theme	b	834:834	arg1	*					835:835	b*	834:835	b*	834:835	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	8	51	theme	packaged	1072:1079	arg1	butter					1081:1086	active packaged butter	1065:1086	active packaged butter	1065:1086	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	2	52	dep	Carum	309:313	arg1	copticum					315:322	copticum	315:322	copticum	315:322	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	0	53	theme	butter	41:46	arg1	control					24:30	Bacterial and oxidative control	0:30	Bacterial and oxidative control of local butter with smart/active film	0:69	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	3	54	dep	morphology	460:469	arg1	The					456:458	The	456:458	The	456:458	The morphology and antibacterial activity of film were studied.					
32998015	8	55	dep	stability	1114:1122	arg1	the					1092:1094	the	1092:1094	the	1092:1094	According to the results active packaged butter had the highest oxidative stability, lowest microbial load, and the least color change during storage.					
32998015	5	56	theme	optimum	710:716	arg1	film					718:721	the optimum film	706:721	the optimum film	706:721	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film was used for packaging of local butter.					
32998015	9	57	theme	packaging	1198:1206	arg1	process					1208:1214	the packaging process	1194:1214	the packaging process	1194:1214	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	58	theme	expiration	1380:1389	arg1	time					1391:1394	expiration time	1380:1394	expiration time	1380:1394	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	6	59	theme	Oxidative	763:771	arg1	stability					773:781	Oxidative stability	763:781	Oxidative stability	763:781	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	3	60	theme	film	501:504	arg1	morphology					460:469	morphology	460:469	morphology	460:469	The morphology and antibacterial activity of film were studied.					
32998015	3	60	theme	film	501:504	arg1	activity					489:496	antibacterial activity	475:496	antibacterial activity	475:496	The morphology and antibacterial activity of film were studied.					
32998015	1	61	theme	composite	246:254	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	0	62	dep	pectin/nanoclay/Carum	80:100	arg1	copticum					102:109	copticum	102:109	copticum	102:109	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	6	63	theme	a	827:827	arg1	*					828:828	a*	827:828	a*	827:828	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	2	64	dep	activity	378:385	arg1	the					362:364	the	362:364	the	362:364	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	65	theme	oils	334:337	arg1	concentration					345:357	Carum copticum Essential oils (CCE) concentration	309:357	Carum copticum Essential oils (CCE) concentration	309:357	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	7	66	dep	coli	1024:1027	arg1	coli					1033:1036	E. coli	1030:1036	E. coli	1030:1036	Results showed that Pec/Clay/CCE/βC film was effective against Bacillus cereus (B. cereus) more than Escherichia coli (E. coli).					
32998015	9	67	theme	butter	1399:1404	arg1	oxidation					1356:1364	the oxidation	1352:1364	the oxidation of butter and expiration time of butter	1352:1404	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	0	68	with	butter	41:46	arg1	film					66:69	smart/active film	53:69	smart/active film	53:69	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	2	69	theme	antioxidant	366:376	arg1	activity					378:385	antioxidant activity	366:385	antioxidant activity	366:385	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	2	70	theme	color/mechanical	391:406	arg1	properties					408:417	color/mechanical properties	391:417	color/mechanical properties	391:417	The effect of β-Carotene (βC) and Carum copticum Essential oils (CCE) concentration on the antioxidant activity and color/mechanical properties of Pec/Clay/CCE/βC film was studied.					
32998015	9	71	theme	smart	1320:1324	arg1	change					1299:1304	this color change	1288:1304	this color change	1288:1304	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	71	theme	smart	1320:1324	arg1	indicator					1332:1340	a smart color indicator	1318:1340	a smart color indicator	1318:1340	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	0	72	theme	smart/active	53:64	arg1	film					66:69	smart/active film	53:69	smart/active film	53:69	Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene.					
32998015	9	73	theme	Pec/Clay/CCE/βC	1217:1231	arg1	color					1238:1242	Pec/Clay/CCE/βC film color	1217:1242	Pec/Clay/CCE/βC film color	1217:1242	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	74	theme	color	1326:1330	arg1	change					1299:1304	this color change	1288:1304	this color change	1288:1304	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	9	74	theme	color	1326:1330	arg1	indicator					1332:1340	a smart color indicator	1318:1340	a smart color indicator	1318:1340	In the packaging process, Pec/Clay/CCE/βC film color was changed from orange to light yellow and this color change was used as a smart color indicator to detect the oxidation of butter and expiration time of butter.					
32998015	1	75	theme	Pectin/Nanoclay	153:167	arg1	film					256:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film	153:259	In this study, Pectin/Nanoclay (montmorillonite)/Carum copticum Essential oils/β-Carotene (Pec/Clay/CCE/βC) composite film was prepared.					
32998015	6	76	dep	stability	773:781	arg1	*					828:828	a*	827:828	a*	827:828	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	6	76	dep	stability	773:781	arg1	*					835:835	b*	834:835	b*	834:835	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	6	76	dep	stability	773:781	arg1	*					824:824	L*	823:824	L*	823:824	Oxidative stability, microbial count, and color properties (L*, a* and b*) of butter packaged with active film (Pec/Clay/CCE/βC) were investigated.					
32998015	5	77	theme	local	749:753	arg1	butter					755:760	local butter	749:760	local butter	749:760	So the Pec/Clay/CCE0.5%/BC0.03% film as the optimum film was used for packaging of local butter.					
32007845	10	0	theme	shelf	1553:1557	arg1	extension					1564:1572	the shelf life extension	1549:1572	the shelf life extension of different perishable foods	1549:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	4	1	theme	vapor	657:661	arg1	permeability					663:674	the water vapor permeability	647:674	the water vapor permeability of WPI film	647:686	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	7	2	theme	physical	1229:1236	arg1	interactions					1238:1249	physical interactions	1229:1249	physical interactions	1229:1249	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
32007845	10	3	theme	bio-nano	1502:1509	arg1	composite					1511:1519	a novel ecofriendly bio-nano composite	1482:1519	a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods	1482:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	6	4	dep	networks	875:882	arg1	contributed					969:979	contributed	969:979	contributed to the reduction of the tensile strength	969:1020	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	6	4	dep	networks	875:882	arg1	distributed					894:904	distributed	894:904	were well distributed throughout the films while the morphological heterogeneity	884:963	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	0	5	theme	chitosan	92:99	arg1	nanofiber					101:109	chitosan nanofiber	92:109	chitosan nanofiber	92:109	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	10	6	theme	foods	1598:1602	arg1	extension					1564:1572	the shelf life extension	1549:1572	the shelf life extension of different perishable foods	1549:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	10	7	theme	perishable	1587:1596	arg1	foods					1598:1602	different perishable foods	1577:1602	different perishable foods	1577:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	2	8	theme	Nanostructured	484:497	arg1	NLC					515:517	NLC	515:517	NLC	515:517	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	8	theme	Nanostructured	484:497	arg1	carriers					505:512	both emulsified and Nanostructured lipid carriers	464:512	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	6	9	theme	fiber	869:873	arg1	evident					1027:1033	evident	1027:1033	evident	1027:1033	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	6	9	theme	fiber	869:873	arg1	networks					875:882	the fiber networks	865:882	the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength	865:1020	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	8	10	theme	ultraviolet	1283:1293	arg1	light					1295:1299	ultraviolet light	1283:1299	ultraviolet light	1283:1299	The film barrier properties to ultraviolet light were increased by adding all of nano-reinforcements.					
32007845	9	11	theme	antibacterial	1368:1380	arg1	activity					1382:1389	the antibacterial activity	1364:1389	the antibacterial activity of resulting films	1364:1408	Moreover, the antibacterial activity of resulting films was enhanced by adding CiEO, especially NLC form.					
32007845	10	12	theme	different	1577:1585	arg1	foods					1598:1602	different perishable foods	1577:1602	different perishable foods	1577:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	8	13	theme	film	1256:1259	arg1	properties					1269:1278	The film barrier properties	1252:1278	The film barrier properties to ultraviolet light	1252:1299	The film barrier properties to ultraviolet light were increased by adding all of nano-reinforcements.					
32007845	2	14	theme	main	288:291	arg1	goal					293:296	The main goal	284:296	The main goal of this research	284:313	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	1	15	theme	products	184:191	arg1	life					199:202	products shelf life	184:202	products shelf life	184:202	Active packaging is designed to extend products shelf life by incorporating active components with biological properties in its structure.					
32007845	2	16	theme	emulsified	469:478	arg1	NLC					515:517	NLC	515:517	NLC	515:517	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	16	theme	emulsified	469:478	arg1	carriers					505:512	both emulsified and Nanostructured lipid carriers	464:512	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	7	17	theme	FT-IR	1122:1126	arg1	findings					1128:1135	FT-IR findings	1122:1135	FT-IR findings	1122:1135	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
32007845	10	18	theme	novel	1484:1488	arg1	composite					1511:1519	a novel ecofriendly bio-nano composite	1482:1519	a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods	1482:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	9	19	theme	films	1404:1408	arg1	activity					1382:1389	the antibacterial activity	1364:1389	the antibacterial activity of resulting films	1364:1408	Moreover, the antibacterial activity of resulting films was enhanced by adding CiEO, especially NLC form.					
32007845	4	20	theme	water	626:630	arg1	solubility					632:641	water solubility	626:641	water solubility	626:641	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	5	21	theme	good	774:777	arg1	complexation					779:790	The good complexation	770:790	The good complexation between WPI and CSNF	770:811	The good complexation between WPI and CSNF was confirmed by FTIR.					
32007845	7	22	from	SEM	1087:1089	arg1	results					1074:1080	These obtained results	1059:1080	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure	1059:1209	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
32007845	7	23	theme	obtained	1065:1072	arg1	results					1074:1080	These obtained results	1059:1080	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure	1059:1209	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
32007845	6	24	theme	CiEO	1053:1056	arg1	addition					1041:1048	addition	1041:1048	addition of CiEO	1041:1056	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	2	25	theme	-based	372:377	arg1	film					379:382	a biodegradable whey protein isolate (WPI)-based film	330:382	a biodegradable whey protein isolate (WPI)-based film	330:382	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	0	26	dep	isolate	66:72	arg1	incorporated					74:85	incorporated	74:85	isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil	66:142	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	1	27	theme	shelf	193:197	arg1	life					199:202	products shelf life	184:202	products shelf life	184:202	Active packaging is designed to extend products shelf life by incorporating active components with biological properties in its structure.					
32007845	0	28	theme	active	21:26	arg1	films					38:42	novel active packaging films	15:42	novel active packaging films based on whey protein	15:64	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	0	29	theme	cinnamon	131:138	arg1	oil					140:142	nano-formulated cinnamon oil	115:142	nano-formulated cinnamon oil	115:142	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	2	30	theme	research	306:313	arg1	goal					293:296	The main goal	284:296	The main goal of this research	284:313	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	8	31	theme	barrier	1261:1267	arg1	properties					1269:1278	The film barrier properties	1252:1278	The film barrier properties to ultraviolet light	1252:1299	The film barrier properties to ultraviolet light were increased by adding all of nano-reinforcements.					
32007845	0	32	theme	novel	15:19	arg1	films					38:42	novel active packaging films	15:42	novel active packaging films based on whey protein	15:64	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	6	33	theme	morphological	937:949	arg1	heterogeneity					951:963	the morphological heterogeneity	933:963	the morphological heterogeneity	933:963	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	0	34	theme	nano-formulated	115:129	arg1	oil					140:142	nano-formulated cinnamon oil	115:142	nano-formulated cinnamon oil	115:142	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	7	35	dep	SEM	1087:1089	arg1	findings					1128:1135	FT-IR findings	1122:1135	FT-IR findings	1122:1135	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
32007845	2	36	theme	chitosan	403:410	arg1	CSNF					423:426	CSNF	423:426	CSNF	423:426	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	36	theme	chitosan	403:410	arg1	nanofiber					412:420	chitosan nanofiber	403:420	chitosan nanofiber (CSNF)	403:427	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	0	37	theme	films	38:42	arg1	Development					0:10	Development	0:10	Development of novel active packaging films based on whey protein	0:64	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	4	38	theme	WPI	679:681	arg1	film					683:686	WPI film	679:686	WPI film	679:686	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	7	39	theme	NLCs	1173:1176	arg1	incorporation					1156:1168	the incorporation	1152:1168	the incorporation of NLCs into bio-nanocomposite structure	1152:1209	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
32007845	3	40	theme	physicochemical	537:551	arg1	properties					553:562	the physicochemical properties	533:562	the physicochemical properties of developed bio-nanocomposite	533:593	Then, the physicochemical properties of developed bio-nanocomposite were fully characterized.					
32007845	0	41	theme	packaging	28:36	arg1	films					38:42	novel active packaging films	15:42	novel active packaging films based on whey protein	15:64	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	2	42	theme	carriers	505:512	arg1	oil					452:454	cinnamon essential oil	433:454	cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form)	433:524	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	42	theme	carriers	505:512	arg1	form					520:523	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	10	43	from	composite	1511:1519	arg1	industries					1534:1543	packaging industries	1524:1543	packaging industries	1524:1543	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	1	44	theme	active	221:226	arg1	components					228:237	active components	221:237	active components	221:237	Active packaging is designed to extend products shelf life by incorporating active components with biological properties in its structure.					
32007845	2	45	theme	essential	442:450	arg1	CiEO					457:460	CiEO	457:460	CiEO	457:460	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	45	theme	essential	442:450	arg1	oil					452:454	cinnamon essential oil	433:454	cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form)	433:524	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	45	theme	essential	442:450	arg1	form					520:523	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	46	theme	lipid	499:503	arg1	NLC					515:517	NLC	515:517	NLC	515:517	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	46	theme	lipid	499:503	arg1	carriers					505:512	both emulsified and Nanostructured lipid carriers	464:512	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	10	47	theme	life	1559:1562	arg1	extension					1564:1572	the shelf life extension	1549:1572	the shelf life extension of different perishable foods	1549:1602	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	4	48	theme	film	683:686	arg1	permeability					663:674	the water vapor permeability	647:674	the water vapor permeability of WPI film	647:686	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	4	48	theme	film	683:686	arg1	solubility					632:641	water solubility	626:641	water solubility	626:641	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	2	49	theme	cinnamon	433:440	arg1	CiEO					457:460	CiEO	457:460	CiEO	457:460	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	49	theme	cinnamon	433:440	arg1	oil					452:454	cinnamon essential oil	433:454	cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form)	433:524	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	2	49	theme	cinnamon	433:440	arg1	form					520:523	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	both emulsified and Nanostructured lipid carriers (NLC) form	464:523	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	1	50	from	properties	255:264	arg1	structure					273:281	its structure	269:281	its structure	269:281	Active packaging is designed to extend products shelf life by incorporating active components with biological properties in its structure.					
32007845	2	51	theme	biodegradable	332:344	arg1	film					379:382	a biodegradable whey protein isolate (WPI)-based film	330:382	a biodegradable whey protein isolate (WPI)-based film	330:382	The main goal of this research was to develop a biodegradable whey protein isolate (WPI)-based film, incorporated with chitosan nanofiber (CSNF) and cinnamon essential oil (CiEO) (both emulsified and Nanostructured lipid carriers (NLC) form).					
32007845	10	52	theme	packaging	1524:1532	arg1	industries					1534:1543	packaging industries	1524:1543	packaging industries	1524:1543	This study introduces a novel ecofriendly bio-nano composite in packaging industries for the shelf life extension of different perishable foods.					
32007845	3	53	theme	bio-nanocomposite	577:593	arg1	properties					553:562	the physicochemical properties	533:562	the physicochemical properties of developed bio-nanocomposite	533:593	Then, the physicochemical properties of developed bio-nanocomposite were fully characterized.					
32007845	9	54	theme	resulting	1394:1402	arg1	films					1404:1408	resulting films	1394:1408	resulting films	1394:1408	Moreover, the antibacterial activity of resulting films was enhanced by adding CiEO, especially NLC form.					
32007845	6	55	theme	strength	1013:1020	arg1	reduction					988:996	the reduction	984:996	the reduction of the tensile strength	984:1020	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	4	56	theme	film	754:757	arg1	structure					759:767	film structure	754:767	film structure	754:767	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	4	57	dep	decreased	688:696	arg1	p < 0.05					713:720	p < 0.05	713:720	p < 0.05	713:720	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	6	58	theme	tensile	1005:1011	arg1	strength					1013:1020	the tensile strength	1001:1020	the tensile strength	1001:1020	Microstructure revealed that the fiber networks were well distributed throughout the films while the morphological heterogeneity and contributed to the reduction of the tensile strength were evident after addition of CiEO.					
32007845	1	59	theme	Active	145:150	arg1	packaging					152:160	Active packaging	145:160	Active packaging	145:160	Active packaging is designed to extend products shelf life by incorporating active components with biological properties in its structure.					
32007845	9	60	theme	NLC	1450:1452	arg1	form					1454:1457	NLC form	1450:1457	NLC form	1450:1457	Moreover, the antibacterial activity of resulting films was enhanced by adding CiEO, especially NLC form.					
32007845	9	60	theme	NLC	1450:1452	arg1	CiEO					1433:1436	CiEO	1433:1436	CiEO	1433:1436	Moreover, the antibacterial activity of resulting films was enhanced by adding CiEO, especially NLC form.					
32007845	1	61	theme	biological	244:253	arg1	properties					255:264	biological properties	244:264	biological properties in its structure	244:281	Active packaging is designed to extend products shelf life by incorporating active components with biological properties in its structure.					
32007845	0	62	theme	whey	53:56	arg1	protein					58:64	whey protein	53:64	whey protein	53:64	Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil.					
32007845	3	63	theme	developed	567:575	arg1	bio-nanocomposite					577:593	developed bio-nanocomposite	567:593	developed bio-nanocomposite	567:593	Then, the physicochemical properties of developed bio-nanocomposite were fully characterized.					
32007845	4	64	theme	water	651:655	arg1	permeability					663:674	the water vapor permeability	647:674	the water vapor permeability of WPI film	647:686	Both water solubility and the water vapor permeability of WPI film decreased significantly (p < 0.05) by incorporating the CSNF into film structure.					
32007845	7	65	theme	bio-nanocomposite	1183:1199	arg1	structure					1201:1209	bio-nanocomposite structure	1183:1209	bio-nanocomposite structure	1183:1209	These obtained results from SEM to be quite in accordance with FT-IR findings that confirmed the incorporation of NLCs into bio-nanocomposite structure have been through physical interactions.					
34463967	0	0	theme	sugarcane	83:91	arg1	bagasse					93:99	ionic liquid pretreated sugarcane bagasse	59:99	ionic liquid pretreated sugarcane bagasse	59:99	Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse.					
34463967	7	1	theme	lignin	976:981	arg1	content					983:989	lignin content	976:989	lignin content	976:989	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	8	2	theme	different	1329:1337	arg1	enzymes					1339:1345	different enzymes	1329:1345	different enzymes	1329:1345	The strategy adopted by utilizing a mixture of IL pretreated and untreated SB under SSF proved promising to obtain high titers of different enzymes simultaneously.					
34463967	0	3	theme	pretreated	72:81	arg1	bagasse					93:99	ionic liquid pretreated sugarcane bagasse	59:99	ionic liquid pretreated sugarcane bagasse	59:99	Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse.					
34463967	6	4	theme	50 IU mg-1	909:918	arg1	protein					920:926	50 IU mg-1 protein	909:926	50 IU mg-1 protein	909:926	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	6	5	theme	Volumetric	810:819	arg1	productivity					834:845	Volumetric and specific productivity	810:845	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein)	810:927	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	6	6	theme	xylanase	850:857	arg1	productivity					834:845	Volumetric and specific productivity	810:845	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein)	810:927	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	7	7	theme	electron	1105:1112	arg1	microscopy					1114:1123	scanning electron microscopy	1096:1123	scanning electron microscopy	1096:1123	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	8	8	theme	enzymes	1339:1345	arg1	titers					1319:1324	high titers	1314:1324	high titers of different enzymes	1314:1345	The strategy adopted by utilizing a mixture of IL pretreated and untreated SB under SSF proved promising to obtain high titers of different enzymes simultaneously.					
34463967	2	9	dep	impediment	344:353	arg1	utilized					431:438	utilized	431:438	was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF)	427:507	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	9	10	theme	commercial	1467:1476	arg1	processes					1478:1486	commercial processes	1467:1486	commercial processes which are carried out at high temperatures	1467:1529	Since the bacterial strain used is thermophilic, therefore, the multienzyme can find its application in commercial processes which are carried out at high temperatures.					
34463967	9	11	theme	high	1513:1516	arg1	temperatures					1518:1529	high temperatures	1513:1529	high temperatures	1513:1529	Since the bacterial strain used is thermophilic, therefore, the multienzyme can find its application in commercial processes which are carried out at high temperatures.					
34463967	7	12	dep	transform	1134:1142	arg1	infrared					1144:1151	infrared	1144:1151	transform infrared spectroscopy	1134:1164	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	8	13	theme	untreated	1264:1272	arg1	SB					1274:1275	IL pretreated and untreated SB	1246:1275	IL pretreated and untreated SB	1246:1275	The strategy adopted by utilizing a mixture of IL pretreated and untreated SB under SSF proved promising to obtain high titers of different enzymes simultaneously.					
34463967	3	14	theme	Bacillus	510:517	arg1	UE25					529:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	3	14	theme	Bacillus	510:517	arg1	strain					550:555	a thermophilic strain	535:555	a thermophilic strain	535:555	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	4	15	theme	composite	649:657	arg1	design					659:664	a central composite design	639:664	a central composite design	639:664	Fermentation conditions were optimized by adopting a central composite design.					
34463967	5	16	theme	experimental	733:744	arg1	values					746:751	the experimental values	729:751	the experimental values	729:751	The model showed a good correlation between the predicted and the experimental values for amylase, xylanase, endoglucanase, and β-glucosidase.					
34463967	6	17	theme	244.25 IU mg-1	879:892	arg1	substrate					894:902	244.25 IU mg-1 substrate	879:902	244.25 IU mg-1 substrate	879:902	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	7	18	theme	scanning	1096:1103	arg1	microscopy					1114:1123	scanning electron microscopy	1096:1123	scanning electron microscopy	1096:1123	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	3	19	theme	aestuarii	519:527	arg1	UE25					529:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	3	19	theme	aestuarii	519:527	arg1	strain					550:555	a thermophilic strain	535:555	a thermophilic strain	535:555	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	1	20	theme	pretreatment	169:180	arg1	processes					182:190	pretreatment processes	169:190	pretreatment processes	169:190	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	7	21	theme	magnetic	1179:1186	arg1	resonance					1188:1196	Nuclear magnetic resonance	1171:1196	Nuclear magnetic resonance	1171:1196	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	6	22	theme	other	950:954	arg1	enzymes					956:962	the other enzymes	946:962	the other enzymes	946:962	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	2	23	theme	SB	424:425	arg1	mixture					385:391	a mixture	383:391	a mixture of IL pretreated and untreated SB	383:425	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	8	24	theme	pretreated	1249:1258	arg1	SB					1274:1275	IL pretreated and untreated SB	1246:1275	IL pretreated and untreated SB	1246:1275	The strategy adopted by utilizing a mixture of IL pretreated and untreated SB under SSF proved promising to obtain high titers of different enzymes simultaneously.					
34463967	2	25	theme	untreated	414:422	arg1	SB					424:425	IL pretreated and untreated SB	396:425	IL pretreated and untreated SB	396:425	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	5	26	theme	good	686:689	arg1	correlation					691:701	a good correlation	684:701	a good correlation between the predicted and the experimental values for amylase, xylanase, endoglucanase, and β-glucosidase	684:807	The model showed a good correlation between the predicted and the experimental values for amylase, xylanase, endoglucanase, and β-glucosidase.					
34463967	2	27	theme	pretreated	399:408	arg1	SB					424:425	IL pretreated and untreated SB	396:425	IL pretreated and untreated SB	396:425	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	0	28	theme	multienzyme	14:24	arg1	Production					0:9	Production	0:9	Production of multienzyme by Bacillus aestuarii UE25	0:51	Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse.					
34463967	3	29	theme	thermophilic	537:548	arg1	UE25					529:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	3	29	theme	thermophilic	537:548	arg1	strain					550:555	a thermophilic strain	535:555	a thermophilic strain	535:555	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	1	30	theme	fermentable	202:212	arg1	components					214:223	fermentable components	202:223	fermentable components available to microorganisms	202:251	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	2	31	theme	bacterial	450:458	arg1	multienzyme					460:470	bacterial multienzyme	450:470	bacterial multienzyme	450:470	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	3	32	used	utilized	561:568	arg2	strain					550:555	a thermophilic strain	535:555	a thermophilic strain	535:555	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	3	32	used	utilized	561:568	arg2	UE25					529:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25	510:532	Bacillus aestuarii UE25, a thermophilic strain was utilized for that purpose.					
34463967	6	33	theme	4580 IU ml-1	860:871	arg1	h-1					873:875	4580 IU ml-1 h-1	860:875	4580 IU ml-1 h-1	860:875	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	7	34	from	Changes	965:971	arg1	content					983:989	lignin content	976:989	lignin content	976:989	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	7	34	from	Changes	965:971	arg1	crystallinity					1013:1025	reduced cellulose crystallinity	995:1025	reduced cellulose crystallinity	995:1025	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	0	35	theme	Bacillus	29:36	arg1	aestuarii					38:46	Bacillus aestuarii UE25	29:51	Bacillus aestuarii UE25	29:51	Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse.					
34463967	1	36	theme	available	225:233	arg1	components					214:223	fermentable components	202:223	fermentable components available to microorganisms	202:251	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	6	37	dep	xylanase	850:857	arg1	substrate					894:902	244.25 IU mg-1 substrate	879:902	244.25 IU mg-1 substrate	879:902	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	6	37	dep	xylanase	850:857	arg1	protein					920:926	50 IU mg-1 protein	909:926	50 IU mg-1 protein	909:926	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	6	37	dep	xylanase	850:857	arg1	h-1					873:875	4580 IU ml-1 h-1	860:875	4580 IU ml-1 h-1	860:875	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	2	38	theme	solid-state	478:488	arg1	SSF					504:506	SSF	504:506	SSF	504:506	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	2	38	theme	solid-state	478:488	arg1	fermentation					490:501	solid-state fermentation	478:501	solid-state fermentation (SSF)	478:507	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	7	39	dep	Fourier	1126:1132	arg1	transform					1134:1142	transform	1134:1142	transform infrared spectroscopy	1134:1164	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	7	40	theme	reduced	995:1001	arg1	crystallinity					1013:1025	reduced cellulose crystallinity	995:1025	reduced cellulose crystallinity	995:1025	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	1	41	theme	sugarcane	121:129	arg1	bagasse					131:137	sugarcane bagasse	121:137	sugarcane bagasse (SB)	121:142	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	1	41	theme	sugarcane	121:129	arg1	SB					140:141	SB	140:141	SB	140:141	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	7	42	theme	cellulose	1003:1011	arg1	crystallinity					1013:1025	reduced cellulose crystallinity	995:1025	reduced cellulose crystallinity	995:1025	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	1	43	theme	bagasse	131:137	arg1	utilization					106:116	The utilization	102:116	The utilization of sugarcane bagasse (SB) in fermentation	102:158	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	1	44	from	utilization	106:116	arg1	fermentation					147:158	fermentation	147:158	fermentation	147:158	The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms.					
34463967	4	45	theme	Fermentation	588:599	arg1	conditions					601:610	Fermentation conditions	588:610	Fermentation conditions	588:610	Fermentation conditions were optimized by adopting a central composite design.					
34463967	7	46	theme	IL	1034:1035	arg1	pretreatment					1037:1048	IL pretreatment	1034:1048	IL pretreatment	1034:1048	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	6	47	theme	specific	825:832	arg1	productivity					834:845	Volumetric and specific productivity	810:845	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein)	810:927	Volumetric and specific productivity of xylanase (4580 IU ml-1 h-1 , 244.25 IU mg-1 substrate, and 50 IU mg-1 protein) were higher than the other enzymes.					
34463967	2	48	theme	ionic	276:280	arg1	ILs					291:293	ILs	291:293	ILs	291:293	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	2	48	theme	ionic	276:280	arg1	liquids					282:288	ionic liquids	276:288	ionic liquids (ILs)	276:294	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	9	49	from	application	1452:1462	arg1	processes					1478:1486	commercial processes	1467:1486	commercial processes which are carried out at high temperatures	1467:1529	Since the bacterial strain used is thermophilic, therefore, the multienzyme can find its application in commercial processes which are carried out at high temperatures.					
34463967	2	50	theme	high	328:331	arg1	cost					333:336	the high cost	324:336	the high cost	324:336	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	2	50	theme	high	328:331	arg1	impediment					344:353	an impediment	341:353	an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF)	341:507	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	0	51	theme	liquid	65:70	arg1	bagasse					93:99	ionic liquid pretreated sugarcane bagasse	59:99	ionic liquid pretreated sugarcane bagasse	59:99	Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse.					
34463967	8	52	theme	SB	1274:1275	arg1	mixture					1235:1241	a mixture	1233:1241	a mixture of IL pretreated and untreated SB	1233:1275	The strategy adopted by utilizing a mixture of IL pretreated and untreated SB under SSF proved promising to obtain high titers of different enzymes simultaneously.					
34463967	4	53	theme	central	641:647	arg1	design					659:664	a central composite design	639:664	a central composite design	639:664	Fermentation conditions were optimized by adopting a central composite design.					
34463967	0	54	theme	ionic	59:63	arg1	bagasse					93:99	ionic liquid pretreated sugarcane bagasse	59:99	ionic liquid pretreated sugarcane bagasse	59:99	Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse.					
34463967	2	55	used	utilized	431:438	arg2	adoption					362:369	its adoption	358:369	its adoption	358:369	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	2	55	used	utilized	431:438	arg2	mixture					385:391	a mixture	383:391	a mixture of IL pretreated and untreated SB	383:425	Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain bacterial multienzyme under solid-state fermentation (SSF).					
34463967	7	56	theme	Nuclear	1171:1177	arg1	resonance					1188:1196	Nuclear magnetic resonance	1171:1196	Nuclear magnetic resonance	1171:1196	Changes in lignin content and reduced cellulose crystallinity due to IL pretreatment, followed by fermentation, were visualized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Nuclear magnetic resonance.					
34463967	8	57	theme	high	1314:1317	arg1	titers					1319:1324	high titers	1314:1324	high titers of different enzymes	1314:1345	The strategy adopted by utilizing a mixture of IL pretreated and untreated SB under SSF proved promising to obtain high titers of different enzymes simultaneously.					
34463967	9	58	theme	bacterial	1373:1381	arg1	strain					1383:1388	the bacterial strain	1369:1388	the bacterial strain used	1369:1393	Since the bacterial strain used is thermophilic, therefore, the multienzyme can find its application in commercial processes which are carried out at high temperatures.					
34463967	9	58	theme	bacterial	1373:1381	arg1	thermophilic					1398:1409	thermophilic	1398:1409	thermophilic	1398:1409	Since the bacterial strain used is thermophilic, therefore, the multienzyme can find its application in commercial processes which are carried out at high temperatures.					
34626523	5	0	theme	IR	729:730	arg1	spectra					732:738	the IR spectra	725:738	the IR spectra	725:738	By analyzing the IR spectra and the morphology, the synergistic effect of hydrogen bonds between molecules and cracks significantly improves the mechanical properties of the complex.					
34626523	5	1	theme	synergistic	764:774	arg1	effect					776:781	the synergistic effect	760:781	the synergistic effect of hydrogen bonds between molecules and cracks	760:828	By analyzing the IR spectra and the morphology, the synergistic effect of hydrogen bonds between molecules and cracks significantly improves the mechanical properties of the complex.					
34626523	1	2	theme	artificial	178:187	arg1	skins					189:193	artificial skins	178:193	artificial skins	178:193	The mechanical properties of artificial skins are complicated to maintain under ensuring air permeability and antimicrobial.					
34626523	4	3	theme	strong	657:662	arg1	abilities					675:683	strong mechanical abilities	657:683	strong mechanical abilities	657:683	The CS/PVA/LPB/2D-HOF complex exhibits strong mechanical abilities than other control groups.					
34626523	5	4	theme	mechanical	857:866	arg1	properties					868:877	the mechanical properties	853:877	the mechanical properties of the complex	853:892	By analyzing the IR spectra and the morphology, the synergistic effect of hydrogen bonds between molecules and cracks significantly improves the mechanical properties of the complex.					
34626523	8	5	from	investigations	1265:1278	arg1	skin					1298:1301	the artificial skin	1283:1301	the artificial skin	1283:1301	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	8	5	from	investigations	1265:1278	arg1	engineering					1314:1324	tissue engineering	1307:1324	tissue engineering	1307:1324	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	6	6	theme	tensile	907:913	arg1	strength					915:922	Its maximum tensile strength	895:922	Its maximum tensile strength	895:922	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	1	7	theme	skins	189:193	arg1	properties					164:173	The mechanical properties	149:173	The mechanical properties of artificial skins	149:193	The mechanical properties of artificial skins are complicated to maintain under ensuring air permeability and antimicrobial.					
34626523	0	8	theme	Mechanical	112:121	arg1	Skin					143:146	High Mechanical Strength Artificial Skin	107:146	High Mechanical Strength Artificial Skin	107:146	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	6	9	theme	maximum	899:905	arg1	strength					915:922	Its maximum tensile strength	895:922	Its maximum tensile strength	895:922	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	2	10	theme	networks	326:333	arg1	series					282:287	a series	280:287	a series of hydrophilic antimicrobial polymer networks	280:333	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	0	11	theme	High	107:110	arg1	Skin					143:146	High Mechanical Strength Artificial Skin	107:146	High Mechanical Strength Artificial Skin	107:146	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	6	12	theme	antimicrobial	1049:1061	arg1	activity					1063:1070	an excellent antimicrobial activity	1036:1070	an excellent antimicrobial activity	1036:1070	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	2	13	theme	polymer	318:324	arg1	networks					326:333	hydrophilic antimicrobial polymer networks	292:333	hydrophilic antimicrobial polymer networks	292:333	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	4	14	theme	mechanical	664:673	arg1	abilities					675:683	strong mechanical abilities	657:683	strong mechanical abilities	657:683	The CS/PVA/LPB/2D-HOF complex exhibits strong mechanical abilities than other control groups.					
34626523	0	15	theme	Artificial	132:141	arg1	Skin					143:146	High Mechanical Strength Artificial Skin	107:146	High Mechanical Strength Artificial Skin	107:146	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	2	16	with	framework	454:462	arg1	betaine					420:426	the lauramidopropyl betaine	400:426	the lauramidopropyl betaine	400:426	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	2	17	theme	antimicrobial	304:316	arg1	networks					326:333	hydrophilic antimicrobial polymer networks	292:333	hydrophilic antimicrobial polymer networks	292:333	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	2	18	theme	organic	446:452	arg1	CS/PVA/LPB/2D-HOF					465:481	CS/PVA/LPB/2D-HOF	465:481	CS/PVA/LPB/2D-HOF	465:481	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	2	18	theme	organic	446:452	arg1	framework					454:462	hydrogen bond organic framework	432:462	hydrogen bond organic framework (CS/PVA/LPB/2D-HOF)	432:482	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	0	19	theme	Strength	123:130	arg1	Skin					143:146	High Mechanical Strength Artificial Skin	107:146	High Mechanical Strength Artificial Skin	107:146	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	6	20	theme	load	958:961	arg1	capacity					963:970	its maximum load capacity	946:970	its maximum load capacity	946:970	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	7	21	theme	wound	1172:1176	arg1	95％					1187:1189	95％	1187:1189	95％	1187:1189	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	7	21	theme	wound	1172:1176	arg1	healing					1178:1184	wound healing	1172:1184	wound healing (95％)	1172:1190	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	2	22	theme	hydrophilic	292:302	arg1	networks					326:333	hydrophilic antimicrobial polymer networks	292:333	hydrophilic antimicrobial polymer networks	292:333	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	7	23	dep	in	1085:1086	arg1	vitro					1088:1092	vitro	1088:1092	vitro	1088:1092	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	2	24	theme	bond	441:444	arg1	CS/PVA/LPB/2D-HOF					465:481	CS/PVA/LPB/2D-HOF	465:481	CS/PVA/LPB/2D-HOF	465:481	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	2	24	theme	bond	441:444	arg1	framework					454:462	hydrogen bond organic framework	432:462	hydrogen bond organic framework (CS/PVA/LPB/2D-HOF)	432:482	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	0	25	theme	Alcohol/	19:26	arg1	Film					65:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film	0:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds	0:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	6	26	theme	maximum	950:956	arg1	capacity					963:970	its maximum load capacity	946:970	its maximum load capacity	946:970	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	7	27	theme	tissue	1148:1153	arg1	regeneration					1155:1166	tissue regeneration	1148:1166	tissue regeneration	1148:1166	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	6	28	dep	reach	928:932	arg1	g.					987:988	g.	987:988	g.	987:988	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	2	29	theme	hydrogen	432:439	arg1	bond					441:444	hydrogen bond	432:444	hydrogen bond organic framework (CS/PVA/LPB/2D-HOF)	432:482	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	0	30	theme	Chitosan/Polyvinyl	0:17	arg1	Film					65:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film	0:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds	0:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	6	31	theme	excellent	1039:1047	arg1	activity					1063:1070	an excellent antimicrobial activity	1036:1070	an excellent antimicrobial activity	1036:1070	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	8	32	theme	tissue	1307:1312	arg1	engineering					1314:1324	tissue engineering	1307:1324	tissue engineering	1307:1324	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	0	33	theme	Betaine/2D-HOF	44:57	arg1	Film					65:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film	0:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds	0:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	3	34	theme	control	519:525	arg1	groups					527:532	the control groups	515:532	the control groups	515:532	The mechanical performance of the control groups and the complex are systematically evaluated to attain an artificial strength skin.					
34626523	0	35	theme	Lauramidopropyl	28:42	arg1	Film					65:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film	0:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds	0:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	7	36	theme	hybrid	1120:1125	arg1	membrane					1127:1134	the hybrid membrane	1116:1134	the hybrid membrane	1116:1134	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	8	37	dep	skin	1298:1301	arg1	field					1326:1330	field	1326:1330	field	1326:1330	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	3	38	theme	groups	527:532	arg1	complex					542:548	the complex	538:548	the complex	538:548	The mechanical performance of the control groups and the complex are systematically evaluated to attain an artificial strength skin.					
34626523	3	38	theme	groups	527:532	arg1	performance					500:510	The mechanical performance	485:510	The mechanical performance of the control groups	485:532	The mechanical performance of the control groups and the complex are systematically evaluated to attain an artificial strength skin.					
34626523	4	39	theme	CS/PVA/LPB/2D-HOF	622:638	arg1	complex					640:646	The CS/PVA/LPB/2D-HOF complex	618:646	The CS/PVA/LPB/2D-HOF complex	618:646	The CS/PVA/LPB/2D-HOF complex exhibits strong mechanical abilities than other control groups.					
34626523	5	40	theme	hydrogen	786:793	arg1	bonds					795:799	hydrogen bonds	786:799	hydrogen bonds between molecules and cracks	786:828	By analyzing the IR spectra and the morphology, the synergistic effect of hydrogen bonds between molecules and cracks significantly improves the mechanical properties of the complex.					
34626523	2	41	with	chitosan	364:371	arg1	betaine					420:426	the lauramidopropyl betaine	400:426	the lauramidopropyl betaine	400:426	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	7	42	dep	In	1073:1074	arg1	vivo					1076:1079	vivo	1076:1079	vivo	1076:1079	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	1	43	theme	air	238:240	arg1	permeability					242:253	air permeability	238:253	air permeability	238:253	The mechanical properties of artificial skins are complicated to maintain under ensuring air permeability and antimicrobial.					
34626523	0	44	theme	Mixed	59:63	arg1	Film					65:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film	0:68	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds	0:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	5	45	theme	bonds	795:799	arg1	effect					776:781	the synergistic effect	760:781	the synergistic effect of hydrogen bonds between molecules and cracks	760:828	By analyzing the IR spectra and the morphology, the synergistic effect of hydrogen bonds between molecules and cracks significantly improves the mechanical properties of the complex.					
34626523	2	46	with	alcohol	387:393	arg1	betaine					420:426	the lauramidopropyl betaine	400:426	the lauramidopropyl betaine	400:426	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	8	47	theme	material	1256:1263	arg1	investigations					1265:1278	future composite material investigations	1239:1278	future composite material investigations in the artificial skin and tissue engineering field	1239:1330	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	7	48	theme	in	1085:1086	arg1	experiments					1094:1104	In vivo and in vitro experiments	1073:1104	In vivo and in vitro experiments	1073:1104	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	8	49	theme	future	1239:1244	arg1	investigations					1265:1278	future composite material investigations	1239:1278	future composite material investigations in the artificial skin and tissue engineering field	1239:1330	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	0	50	theme	Abundant	75:82	arg1	Bonds					93:97	Abundant Hydrogen Bonds	75:97	Abundant Hydrogen Bonds	75:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	6	51	theme	composite	1003:1011	arg1	membrane					1013:1020	the composite membrane	999:1020	the composite membrane	999:1020	Its maximum tensile strength can reach 29 MPa, and its maximum load capacity can reach 3700 g. Notably, the composite membrane also performs an excellent antimicrobial activity.					
34626523	3	52	theme	mechanical	489:498	arg1	performance					500:510	The mechanical performance	485:510	The mechanical performance of the control groups	485:532	The mechanical performance of the control groups and the complex are systematically evaluated to attain an artificial strength skin.					
34626523	5	53	theme	complex	886:892	arg1	properties					868:877	the mechanical properties	853:877	the mechanical properties of the complex	853:892	By analyzing the IR spectra and the morphology, the synergistic effect of hydrogen bonds between molecules and cracks significantly improves the mechanical properties of the complex.					
34626523	2	54	theme	polyvinyl	377:385	arg1	alcohol					387:393	polyvinyl alcohol	377:393	polyvinyl alcohol	377:393	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	4	55	theme	other	690:694	arg1	groups					704:709	other control groups	690:709	other control groups	690:709	The CS/PVA/LPB/2D-HOF complex exhibits strong mechanical abilities than other control groups.					
34626523	3	56	theme	artificial	592:601	arg1	skin					612:615	an artificial strength skin	589:615	an artificial strength skin	589:615	The mechanical performance of the control groups and the complex are systematically evaluated to attain an artificial strength skin.					
34626523	3	57	theme	strength	603:610	arg1	skin					612:615	an artificial strength skin	589:615	an artificial strength skin	589:615	The mechanical performance of the control groups and the complex are systematically evaluated to attain an artificial strength skin.					
34626523	1	58	theme	mechanical	153:162	arg1	properties					164:173	The mechanical properties	149:173	The mechanical properties of artificial skins	149:193	The mechanical properties of artificial skins are complicated to maintain under ensuring air permeability and antimicrobial.					
34626523	8	59	theme	artificial	1287:1296	arg1	skin					1298:1301	the artificial skin	1283:1301	the artificial skin	1283:1301	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	7	60	theme	In	1073:1074	arg1	experiments					1094:1104	In vivo and in vitro experiments	1073:1104	In vivo and in vitro experiments	1073:1104	In vivo and in vitro experiments show that the hybrid membrane can promote tissue regeneration and wound healing (95％).					
34626523	0	61	theme	Hydrogen	84:91	arg1	Bonds					93:97	Abundant Hydrogen Bonds	75:97	Abundant Hydrogen Bonds	75:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	8	62	theme	composite	1246:1254	arg1	investigations					1265:1278	future composite material investigations	1239:1278	future composite material investigations in the artificial skin and tissue engineering field	1239:1330	These results may open up the opportunity for future composite material investigations in the artificial skin and tissue engineering field.					
34626523	0	63	with	Film	65:68	arg1	Bonds					93:97	Abundant Hydrogen Bonds	75:97	Abundant Hydrogen Bonds	75:97	Chitosan/Polyvinyl Alcohol/ Lauramidopropyl Betaine/2D-HOF Mixed Film with Abundant Hydrogen Bonds Acts as High Mechanical Strength Artificial Skin.					
34626523	2	64	theme	lauramidopropyl	404:418	arg1	betaine					420:426	the lauramidopropyl betaine	400:426	the lauramidopropyl betaine	400:426	Thus, a series of hydrophilic antimicrobial polymer networks are prepared by crosslinking chitosan and polyvinyl alcohol with the lauramidopropyl betaine and hydrogen bond organic framework (CS/PVA/LPB/2D-HOF).					
34626523	4	65	theme	control	696:702	arg1	groups					704:709	other control groups	690:709	other control groups	690:709	The CS/PVA/LPB/2D-HOF complex exhibits strong mechanical abilities than other control groups.					
34428488	6	0	theme	Composite	922:930	arg1	CCRD					947:950	CCRD	947:950	CCRD	947:950	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	0	theme	Composite	922:930	arg1	Design					939:944	A Central Composite Rotary Design	912:944	A Central Composite Rotary Design (CCRD)	912:951	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	2	1	theme	reserve	331:337	arg1	polysaccharide					339:352	reserve polysaccharide	331:352	reserve polysaccharide in the roots of several low-requirement crops	331:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	3	2	theme	substrate	460:468	arg1	"					481:481	a "substrate facilitator"	457:481	a "substrate facilitator" microbial consortium	457:502	In this work, a "substrate facilitator" microbial consortium was designed to address PHA production using inulin as feedstock.					
34428488	1	3	theme	biomass	227:233	arg1	feedstocks					235:244	renewable, non-food biomass feedstocks	207:244	renewable, non-food biomass feedstocks	207:244	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	0	4	theme	inulin	75:80	arg1	conversion					61:70	the conversion	57:70	the conversion of inulin into Polyhydroxyalkanoates	57:107	The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates.					
34428488	5	5	theme	PHA	856:858	arg1	producer					860:867	an established PHA producer	841:867	an established PHA producer	841:867	RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin.					
34428488	5	5	theme	PHA	856:858	arg1	necator					832:838	Cupriavidus necator	820:838	Cupriavidus necator	820:838	RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin.					
34428488	7	6	theme	Polyhydroxybutyrate	1194:1212	arg1	1.9 g L-1					1181:1189	1.9 g L-1	1181:1189	1.9 g L-1	1181:1189	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	4	7	theme	selected	681:688	arg1	strain					690:695	the selected strain	677:695	the selected strain	677:695	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	6	8	theme	microbial	1030:1038	arg1	consortium					1040:1049	the designed artificial microbial consortium	1006:1049	the designed artificial microbial consortium	1006:1049	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	3	9	theme	microbial	483:491	arg1	consortium					493:502	a "substrate facilitator" microbial consortium	457:502	a "substrate facilitator" microbial consortium	457:502	In this work, a "substrate facilitator" microbial consortium was designed to address PHA production using inulin as feedstock.					
34428488	6	10	theme	designed	1010:1017	arg1	consortium					1040:1049	the designed artificial microbial consortium	1006:1049	the designed artificial microbial consortium	1006:1049	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	1	11	theme	microbial	142:150	arg1	production					152:161	the microbial production	138:161	the microbial production of polyhydroxyalkanoates (PHA)	138:192	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	7	12	theme	optimized	1146:1154	arg1	conditions					1156:1165	the optimized conditions	1142:1165	the optimized conditions	1142:1165	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	6	13	theme	N-source	1114:1121	arg1	concentrations					1123:1136	N-source concentrations	1114:1136	N-source concentrations	1114:1136	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	8	14	theme	optimization	1347:1358	arg1	method					1360:1365	an effective process optimization method	1326:1365	an effective process optimization method	1326:1365	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	6	15	theme	artificial	1019:1028	arg1	consortium					1040:1049	the designed artificial microbial consortium	1006:1049	the designed artificial microbial consortium	1006:1049	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	3	16	theme	PHA	528:530	arg1	production					532:541	PHA production	528:541	PHA production	528:541	In this work, a "substrate facilitator" microbial consortium was designed to address PHA production using inulin as feedstock.					
34428488	4	17	theme	inulinase	640:648	arg1	producer					650:657	efficient inulinase producer	630:657	efficient inulinase producer	630:657	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	8	18	theme	C-	1435:1436	arg1	sources					1438:1444	other complex C- sources	1421:1444	other complex C- sources	1421:1444	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	5	19	theme	Cupriavidus	820:830	arg1	producer					860:867	an established PHA producer	841:867	an established PHA producer	841:867	RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin.					
34428488	5	19	theme	Cupriavidus	820:830	arg1	necator					832:838	Cupriavidus necator	820:838	Cupriavidus necator	820:838	RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin.					
34428488	1	20	theme	polyhydroxyalkanoates	166:186	arg1	production					152:161	the microbial production	138:161	the microbial production of polyhydroxyalkanoates (PHA)	138:192	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	0	21	theme	two	13:15	arg1	power					4:8	The power	0:8	The power of two	0:15	The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates.					
34428488	6	22	theme	Rotary	932:937	arg1	CCRD					947:950	CCRD	947:950	CCRD	947:950	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	22	theme	Rotary	932:937	arg1	Design					939:944	A Central Composite Rotary Design	912:944	A Central Composite Rotary Design (CCRD)	912:951	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	2	23	from	polysaccharide	339:352	arg1	roots					361:365	the roots	357:365	the roots of several low-requirement crops	357:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	8	24	theme	effective	1329:1337	arg1	method					1360:1365	an effective process optimization method	1326:1365	an effective process optimization method	1326:1365	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	4	25	theme	catabolic	772:780	arg1	potential					782:790	its wide catabolic potential	763:790	its wide catabolic potential	763:790	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	4	26	theme	Bacillus	596:603	arg1	species					605:611	Bacillus species	596:611	Bacillus species	596:611	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	1	27	theme	fermentation	258:269	arg1	process					271:277	the fermentation process	254:277	the fermentation process	254:277	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	8	28	theme	investigated	1293:1304	arg1	approach					1306:1313	The investigated approach	1289:1313	The investigated approach	1289:1313	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	1	29	dep	renewable	207:215	arg1	non-food					218:225	non-food	218:225	non-food	218:225	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	0	30	dep	consortium	42:51	arg1	power					4:8	The power	0:8	The power of two	0:15	The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates.					
34428488	3	31	theme	facilitator	470:480	arg1	"					481:481	a "substrate facilitator"	457:481	a "substrate facilitator" microbial consortium	457:502	In this work, a "substrate facilitator" microbial consortium was designed to address PHA production using inulin as feedstock.					
34428488	7	32	theme	%	1241:1241	arg1	content					1267:1273	~80% (gpolymer/gCDW) polymer content	1238:1273	~80% (gpolymer/gCDW) polymer content	1238:1273	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	6	33	theme	Central	914:920	arg1	CCRD					947:950	CCRD	947:950	CCRD	947:950	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	33	theme	Central	914:920	arg1	Design					939:944	A Central Composite Rotary Design	912:944	A Central Composite Rotary Design (CCRD)	912:951	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	7	34	theme	~80	1238:1240	arg1	%					1241:1241	%	1241:1241	%	1241:1241	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	4	35	theme	species	605:611	arg1	collection					582:591	A microbial collection	570:591	A microbial collection of Bacillus species	570:611	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	4	36	theme	efficient	630:638	arg1	producer					650:657	efficient inulinase producer	630:657	efficient inulinase producer	630:657	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	2	37	theme	crops	394:398	arg1	roots					361:365	the roots	357:365	the roots of several low-requirement crops	357:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	2	38	theme	several	370:376	arg1	crops					394:398	several low-requirement crops	370:398	several low-requirement crops	370:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	4	39	theme	wide	767:770	arg1	potential					782:790	its wide catabolic potential	763:790	its wide catabolic potential	763:790	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	2	40	theme	low-requirement	378:392	arg1	crops					394:398	several low-requirement crops	370:398	several low-requirement crops	370:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	0	41	theme	microbial	32:40	arg1	consortium					42:51	An artificial microbial consortium	18:51	An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates	18:107	The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates.					
34428488	5	42	theme	established	844:854	arg1	producer					860:867	an established PHA producer	841:867	an established PHA producer	841:867	RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin.					
34428488	5	42	theme	established	844:854	arg1	necator					832:838	Cupriavidus necator	820:838	Cupriavidus necator	820:838	RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin.					
34428488	8	43	from	sources	1438:1444	arg1	production					1398:1407	the production	1394:1407	the production of PHA from other complex C- sources	1394:1444	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	8	44	theme	PHA	1412:1414	arg1	production					1398:1407	the production	1394:1407	the production of PHA from other complex C- sources	1394:1444	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	6	45	theme	inoculum	1084:1091	arg1	ratio					1093:1097	inoculum ratio	1084:1097	inoculum ratio	1084:1097	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	45	theme	inoculum	1084:1091	arg1	species					1076:1082	species inoculum ratio and inulin	1076:1108	species inoculum ratio and inulin	1076:1108	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	0	46	theme	artificial	21:30	arg1	consortium					42:51	An artificial microbial consortium	18:51	An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates	18:107	The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates.					
34428488	7	47	theme	polymer	1259:1265	arg1	content					1267:1273	~80% (gpolymer/gCDW) polymer content	1238:1273	~80% (gpolymer/gCDW) polymer content	1238:1273	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	8	48	theme	complex	1427:1433	arg1	sources					1438:1444	other complex C- sources	1421:1444	other complex C- sources	1421:1444	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	1	49	theme	issues	127:132	arg1	issues					127:132	the major issues	117:132	the major issues for the microbial production of polyhydroxyalkanoates (PHA)	117:192	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	1	49	theme	issues	127:132	arg1	One					110:112	One	110:112	One	110:112	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	6	50	theme	species	1076:1082	arg1	concentrations					1123:1136	N-source concentrations	1114:1136	N-source concentrations	1114:1136	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	50	theme	species	1076:1082	arg1	impact					1066:1071	the impact	1062:1071	the impact of species inoculum ratio and inulin	1062:1108	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	8	51	theme	other	1421:1425	arg1	sources					1438:1444	other complex C- sources	1421:1444	other complex C- sources	1421:1444	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	8	52	theme	process	1339:1345	arg1	method					1360:1365	an effective process optimization method	1326:1365	an effective process optimization method	1326:1365	The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex C- sources.					
34428488	4	53	theme	strain	690:695	arg1	genome					667:672	the genome	663:672	the genome of the selected strain	663:695	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	4	53	theme	strain	690:695	arg1	RHF15					698:702	RHF15	698:702	RHF15	698:702	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
34428488	6	54	theme	PHA	977:979	arg1	synthesis					981:989	PHA synthesis	977:989	PHA synthesis	977:989	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	7	55	dep	%	1241:1241	arg1	gpolymer/gCDW					1244:1256	gpolymer/gCDW	1244:1256	gpolymer/gCDW	1244:1256	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	2	56	theme	polydisperse	290:301	arg1	Inulin					280:285	Inulin	280:285	Inulin	280:285	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	2	56	theme	polydisperse	290:301	arg1	fructan					303:309	a polydisperse fructan	288:309	a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops	288:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	2	57	contain	has	401:403	arg1	Inulin					280:285	Inulin	280:285	Inulin	280:285	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	2	57	contain	has	401:403	arg1	fructan					303:309	a polydisperse fructan	288:309	a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops	288:398	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	2	57	contain	has	401:403	arg2	potential					409:417	the potential to face this challenge	405:440	the potential to face this challenge	405:440	Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.					
34428488	1	58	theme	major	121:125	arg1	issues					127:132	the major issues	117:132	the major issues for the microbial production of polyhydroxyalkanoates (PHA)	117:192	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	1	59	theme	renewable	207:215	arg1	feedstocks					235:244	renewable, non-food biomass feedstocks	207:244	renewable, non-food biomass feedstocks	207:244	One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process.					
34428488	7	60	theme	1.9 g L-1	1181:1189	arg1	maximum					1170:1176	a maximum	1168:1176	a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content	1168:1273	In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to ~80% (gpolymer/gCDW) polymer content was achieved.					
34428488	6	61	dep	species	1076:1082	arg1	inulin					1103:1108	inulin	1103:1108	inulin	1103:1108	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	61	dep	species	1076:1082	arg1	ratio					1093:1097	inoculum ratio	1084:1097	inoculum ratio	1084:1097	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	6	61	dep	species	1076:1082	arg1	species					1076:1082	species inoculum ratio and inulin	1076:1108	species inoculum ratio and inulin	1076:1108	A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and N-source concentrations.					
34428488	3	62	theme	"	481:481	arg1	consortium					493:502	a "substrate facilitator" microbial consortium	457:502	a "substrate facilitator" microbial consortium	457:502	In this work, a "substrate facilitator" microbial consortium was designed to address PHA production using inulin as feedstock.					
34428488	4	63	theme	microbial	572:580	arg1	collection					582:591	A microbial collection	570:591	A microbial collection of Bacillus species	570:611	A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential.					
33543561	7	0	theme	developed	1324:1332	arg1	scaffolds					1344:1352	The developed composite scaffolds	1320:1352	The developed composite scaffolds	1320:1352	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	4	1	dep	METHODS	610:616	arg1	acetonide					715:723	triamcinolone acetonide	701:723	triamcinolone acetonide (TA)	701:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	1	dep	METHODS	610:616	arg1	scaffold					643:650	A novel porous composite scaffold	618:650	A novel porous composite scaffold	618:650	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	1	dep	METHODS	610:616	arg1	TA					726:727	TA	726:727	TA	726:727	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	1	dep	METHODS	610:616	arg1	collagen-I					686:695	collagen-I	686:695	collagen-I	686:695	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	1	dep	METHODS	610:616	arg1	METHODS					610:616	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	1	dep	METHODS	610:616	arg1	ChCo-TAMS					653:661	ChCo-TAMS	653:661	ChCo-TAMS	653:661	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	6	2	from	scaffolds	1120:1128	arg1	defect					1148:1153	the rat dermal defect and porcine oesophageal ESD model	1133:1187	defect	1148:1153	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	2	from	scaffolds	1120:1128	arg1	model					1183:1187	the rat dermal defect and porcine oesophageal ESD model	1133:1187	model	1183:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	2	3	theme	oesophageal	434:444	arg1	injury					466:471	oesophageal stricture following injury	434:471	oesophageal stricture following injury	434:471	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	7	4	theme	composite	1334:1342	arg1	scaffolds					1344:1352	The developed composite scaffolds	1320:1352	The developed composite scaffolds	1320:1352	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	4	5	theme	lactic-co-glycolic	748:765	arg1	TAMS					794:797	TAMS	794:797	TAMS	794:797	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	5	theme	lactic-co-glycolic	748:765	arg1	microspheres					780:791	poly (lactic-co-glycolic) acid (PLGA) microspheres	742:791	poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS)	742:798	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	1	6	theme	oesophageal	308:318	arg1	strictures					320:329	oesophageal strictures	308:329	oesophageal strictures in patients	308:341	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	0	7	theme	dissection-related	135:152	arg1	stenosis					154:161	endoscopic dissection-related stenosis	124:161	endoscopic dissection-related stenosis in oesophagus	124:175	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	6	8	theme	ESD	1292:1294	arg1	strictures					1296:1305	oesophageal ESD strictures	1280:1305	oesophageal ESD strictures	1280:1305	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	9	theme	Further	1093:1099	arg1	application					1101:1111	Further application	1093:1111	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model	1093:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	0	10	theme	endoscopic	124:133	arg1	stenosis					154:161	endoscopic dissection-related stenosis	124:161	endoscopic dissection-related stenosis in oesophagus	124:175	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	6	11	theme	oesophageal	1280:1290	arg1	strictures					1296:1305	oesophageal ESD strictures	1280:1305	oesophageal ESD strictures	1280:1305	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	12	from	model	1183:1187	arg1	application					1101:1111	Further application	1093:1111	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model	1093:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	2	13	theme	Clinical	344:351	arg1	use					353:355	Clinical use	344:355	Clinical use of glucocorticoids through submucosal injection	344:403	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	4	14	theme	PLGA	774:777	arg1	TAMS					794:797	TAMS	794:797	TAMS	794:797	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	14	theme	PLGA	774:777	arg1	microspheres					780:791	poly (lactic-co-glycolic) acid (PLGA) microspheres	742:791	poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS)	742:798	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	7	15	theme	clinical	1374:1381	arg1	device					1391:1396	a promising clinical medical device	1362:1396	a promising clinical medical device for the prevention of post-operative oesophageal stricture	1362:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	7	16	theme	stricture	1447:1455	arg1	prevention					1406:1415	the prevention	1402:1415	the prevention of post-operative oesophageal stricture	1402:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	4	17	theme	ESD-related	892:902	arg1	stenosis					904:911	oesophageal ESD-related stenosis	880:911	oesophageal ESD-related stenosis	880:911	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	5	18	theme	fibroblasts	1080:1090	arg1	activation					997:1006	the activation	993:1006	the activation of macrophages	993:1021	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	5	18	theme	fibroblasts	1080:1090	arg1	recruitment					1048:1058	chemokine-mediated cell recruitment	1024:1058	chemokine-mediated cell recruitment	1024:1058	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	5	18	theme	fibroblasts	1080:1090	arg1	fibrogenesis					1064:1075	fibrogenesis	1064:1075	fibrogenesis	1064:1075	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	5	19	theme	synthesized	926:936	arg1	biomaterials					938:949	The synthesized biomaterials	922:949	The synthesized biomaterials	922:949	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	2	20	theme	dose	516:519	arg1	loss					521:524	dose loss	516:524	dose loss	516:524	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	6	21	theme	wound	1258:1262	arg1	contracture					1264:1274	wound contracture	1258:1274	wound contracture	1258:1274	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	7	22	theme	medical	1383:1389	arg1	device					1391:1396	a promising clinical medical device	1362:1396	a promising clinical medical device for the prevention of post-operative oesophageal stricture	1362:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	2	23	theme	submucosal	384:393	arg1	injection					395:403	submucosal injection	384:403	submucosal injection	384:403	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	6	24	theme	oesophageal	1167:1177	arg1	model					1183:1187	the rat dermal defect and porcine oesophageal ESD model	1133:1187	model	1183:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	0	25	theme	Biological	0:9	arg1	testing					11:17	Biological testing	0:17	Biological testing of chitosan-collagen-based porous scaffolds	0:61	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	4	26	theme	porous	626:631	arg1	METHODS					610:616	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	26	theme	porous	626:631	arg1	ChCo-TAMS					653:661	ChCo-TAMS	653:661	ChCo-TAMS	653:661	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	26	theme	porous	626:631	arg1	scaffold					643:650	A novel porous composite scaffold	618:650	A novel porous composite scaffold	618:650	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	6	27	from	application	1101:1111	arg1	defect					1148:1153	the rat dermal defect and porcine oesophageal ESD model	1133:1187	defect	1148:1153	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	27	from	application	1101:1111	arg1	model					1183:1187	the rat dermal defect and porcine oesophageal ESD model	1133:1187	model	1183:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	5	28	theme	chemokine-mediated	1024:1041	arg1	recruitment					1048:1058	chemokine-mediated cell recruitment	1024:1058	chemokine-mediated cell recruitment	1024:1058	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	1	29	theme	preferential	231:242	arg1	dissection					211:220	OBJECTIVES Endoscopic submucosal dissection	178:220	OBJECTIVES Endoscopic submucosal dissection (ESD)	178:226	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	29	theme	preferential	231:242	arg1	approach					244:251	a preferential approach	229:251	a preferential approach for early oesophageal neoplasms	229:283	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	0	30	theme	chitosan-collagen-based	22:44	arg1	scaffolds					53:61	chitosan-collagen-based porous scaffolds	22:61	chitosan-collagen-based porous scaffolds	22:61	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	4	31	theme	novel	620:624	arg1	METHODS					610:616	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	31	theme	novel	620:624	arg1	ChCo-TAMS					653:661	ChCo-TAMS	653:661	ChCo-TAMS	653:661	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	31	theme	novel	620:624	arg1	scaffold					643:650	A novel porous composite scaffold	618:650	A novel porous composite scaffold	618:650	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	1	32	from	strictures	320:329	arg1	patients					334:341	patients	334:341	patients	334:341	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	5	33	theme	macrophages	1011:1021	arg1	activation					997:1006	the activation	993:1006	the activation of macrophages	993:1021	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	5	33	theme	macrophages	1011:1021	arg1	recruitment					1048:1058	chemokine-mediated cell recruitment	1024:1058	chemokine-mediated cell recruitment	1024:1058	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	5	33	theme	macrophages	1011:1021	arg1	fibrogenesis					1064:1075	fibrogenesis	1064:1075	fibrogenesis	1064:1075	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	2	34	theme	glucocorticoids	360:374	arg1	use					353:355	Clinical use	344:355	Clinical use of glucocorticoids through submucosal injection	344:403	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	4	35	theme	triamcinolone	701:713	arg1	acetonide					715:723	triamcinolone acetonide	701:723	triamcinolone acetonide (TA)	701:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	35	theme	triamcinolone	701:713	arg1	METHODS					610:616	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	35	theme	triamcinolone	701:713	arg1	TA					726:727	TA	726:727	TA	726:727	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	7	36	theme	post-operative	1420:1433	arg1	stricture					1447:1455	post-operative oesophageal stricture	1420:1455	post-operative oesophageal stricture	1420:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	7	37	theme	promising	1364:1372	arg1	device					1391:1396	a promising clinical medical device	1362:1396	a promising clinical medical device for the prevention of post-operative oesophageal stricture	1362:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	5	38	theme	unique	961:966	arg1	properties					968:977	unique properties	961:977	unique properties	961:977	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	2	39	theme	following	456:464	arg1	injury					466:471	oesophageal stricture following injury	434:471	oesophageal stricture following injury	434:471	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	0	40	theme	scaffolds	53:61	arg1	testing					11:17	Biological testing	0:17	Biological testing of chitosan-collagen-based porous scaffolds	0:61	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	4	41	theme	composite	633:641	arg1	METHODS					610:616	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA)	610:728	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	41	theme	composite	633:641	arg1	ChCo-TAMS					653:661	ChCo-TAMS	653:661	ChCo-TAMS	653:661	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	41	theme	composite	633:641	arg1	scaffold					643:650	A novel porous composite scaffold	618:650	A novel porous composite scaffold	618:650	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	6	42	theme	dermal	1141:1146	arg1	defect					1148:1153	the rat dermal defect and porcine oesophageal ESD model	1133:1187	defect	1148:1153	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	43	theme	porcine	1159:1165	arg1	model					1183:1187	the rat dermal defect and porcine oesophageal ESD model	1133:1187	model	1183:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	1	44	theme	early	257:261	arg1	neoplasms					275:283	early oesophageal neoplasms	257:283	early oesophageal neoplasms	257:283	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	0	45	theme	porous	46:51	arg1	scaffolds					53:61	chitosan-collagen-based porous scaffolds	22:61	chitosan-collagen-based porous scaffolds	22:61	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	1	46	theme	oesophageal	263:273	arg1	neoplasms					275:283	early oesophageal neoplasms	257:283	early oesophageal neoplasms	257:283	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	3	47	theme	glucocorticoid	566:579	arg1	therapy					581:587	glucocorticoid therapy	566:587	glucocorticoid therapy	566:587	Hence, alternatives to glucocorticoid therapy should be developed.					
33543561	4	48	theme	oesophageal	880:890	arg1	stenosis					904:911	oesophageal ESD-related stenosis	880:911	oesophageal ESD-related stenosis	880:911	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	6	49	theme	novel	1207:1211	arg1	scaffolds					1213:1221	these novel scaffolds	1201:1221	these novel scaffolds	1201:1221	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	50	theme	robust	1232:1237	arg1	role					1239:1242	a robust role	1230:1242	a robust role	1230:1242	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	4	51	theme	acid	768:771	arg1	TAMS					794:797	TAMS	794:797	TAMS	794:797	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	51	theme	acid	768:771	arg1	microspheres					780:791	poly (lactic-co-glycolic) acid (PLGA) microspheres	742:791	poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS)	742:798	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	6	52	from	defect	1148:1153	arg1	application					1101:1111	Further application	1093:1111	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model	1093:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	7	53	dep	CONCLUSIONS	1308:1318	arg1	provide					1354:1360	provide	1354:1360	provide a promising clinical medical device for the prevention of post-operative oesophageal stricture	1354:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	0	54	from	stenosis	154:161	arg1	oesophagus					166:175	oesophagus	166:175	oesophagus	166:175	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	4	55	theme	poly	742:745	arg1	TAMS					794:797	TAMS	794:797	TAMS	794:797	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	4	55	theme	poly	742:745	arg1	microspheres					780:791	poly (lactic-co-glycolic) acid (PLGA) microspheres	742:791	poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS)	742:798	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	1	56	theme	OBJECTIVES	178:187	arg1	dissection					211:220	OBJECTIVES Endoscopic submucosal dissection	178:220	OBJECTIVES Endoscopic submucosal dissection (ESD)	178:226	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	56	theme	OBJECTIVES	178:187	arg1	approach					244:251	a preferential approach	229:251	a preferential approach for early oesophageal neoplasms	229:283	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	56	theme	OBJECTIVES	178:187	arg1	ESD					223:225	ESD	223:225	ESD	223:225	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	5	57	theme	cell	1043:1046	arg1	recruitment					1048:1058	chemokine-mediated cell recruitment	1024:1058	chemokine-mediated cell recruitment	1024:1058	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	2	58	contain	has	491:493	arg2	limitations					495:505	limitations	495:505	limitations	495:505	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	2	58	contain	has	491:493	arg2	perforation					530:540	perforation	530:540	perforation	530:540	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	2	58	contain	has	491:493	arg2	loss					521:524	dose loss	516:524	dose loss	516:524	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	2	58	contain	has	491:493	arg1	it					483:484	it	483:484	it	483:484	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
33543561	5	59	dep	RESULTS	914:920	arg1	displayed					951:959	displayed	951:959	displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts	951:1090	RESULTS The synthesized biomaterials displayed unique properties in inhibiting the activation of macrophages, chemokine-mediated cell recruitment and fibrogenesis of fibroblasts.					
33543561	1	60	theme	Endoscopic	189:198	arg1	dissection					211:220	OBJECTIVES Endoscopic submucosal dissection	178:220	OBJECTIVES Endoscopic submucosal dissection (ESD)	178:226	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	60	theme	Endoscopic	189:198	arg1	approach					244:251	a preferential approach	229:251	a preferential approach for early oesophageal neoplasms	229:283	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	60	theme	Endoscopic	189:198	arg1	ESD					223:225	ESD	223:225	ESD	223:225	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	6	61	theme	rat	1137:1139	arg1	defect					1148:1153	the rat dermal defect and porcine oesophageal ESD model	1133:1187	defect	1148:1153	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	4	62	theme	biological	847:856	arg1	testing					858:864	biological testing	847:864	biological testing	847:864	METHODS A novel porous composite scaffold, ChCo-TAMS, composed of chitosan, collagen-I and triamcinolone acetonide (TA) loaded into poly (lactic-co-glycolic) acid (PLGA) microspheres (TAMS), was successfully constructed and subjected to biological testing to ameliorate oesophageal ESD-related stenosis.					
33543561	7	63	theme	oesophageal	1435:1445	arg1	stricture					1447:1455	post-operative oesophageal stricture	1420:1455	post-operative oesophageal stricture	1420:1455	CONCLUSIONS The developed composite scaffolds provide a promising clinical medical device for the prevention of post-operative oesophageal stricture.					
33543561	1	64	theme	submucosal	200:209	arg1	dissection					211:220	OBJECTIVES Endoscopic submucosal dissection	178:220	OBJECTIVES Endoscopic submucosal dissection (ESD)	178:226	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	64	theme	submucosal	200:209	arg1	approach					244:251	a preferential approach	229:251	a preferential approach for early oesophageal neoplasms	229:283	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	1	64	theme	submucosal	200:209	arg1	ESD					223:225	ESD	223:225	ESD	223:225	OBJECTIVES Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients.					
33543561	6	65	theme	ESD	1179:1181	arg1	model					1183:1187	the rat dermal defect and porcine oesophageal ESD model	1133:1187	model	1183:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	6	66	theme	scaffolds	1120:1128	arg1	application					1101:1111	Further application	1093:1111	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model	1093:1187	Further application of the scaffolds in the rat dermal defect and porcine oesophageal ESD model showed that these novel scaffolds played a robust role in inhibiting wound contracture and oesophageal ESD strictures.					
33543561	0	67	theme	PLGA/Triamcinolone	75:92	arg1	microspheres					94:105	PLGA/Triamcinolone microspheres	75:105	PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus	75:175	Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.					
33543561	2	68	theme	stricture	446:454	arg1	injury					466:471	oesophageal stricture following injury	434:471	oesophageal stricture following injury	434:471	Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation.					
32843009	12	0	theme	senegalensis	1915:1926	arg1	MA1					1928:1930	A. senegalensis MA1	1912:1930	A. senegalensis MA1	1912:1930	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	7	1	theme	cellulose	822:830	arg1	production					832:841	The maximum cellulose production	810:841	The maximum cellulose production by A. senengalensis MA1	810:865	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	10	2	theme	yeast	1646:1650	arg1	extract					1652:1658	yeast extract	1646:1658	yeast extract	1646:1658	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	12	3	theme	bacterial	1889:1897	arg1	cellulose					1899:1907	bacterial cellulose	1889:1907	bacterial cellulose	1889:1907	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	12	4	theme	unoptimized	1993:2003	arg1	medium					2008:2013	an unoptimized HS medium	1990:2013	an unoptimized HS medium	1990:2013	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	1	5	theme	large	176:180	arg1	quantities					182:191	large quantities	176:191	large quantities from plants	176:203	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	12	6	theme	medium	1847:1852	arg1	conditions					1826:1835	The optimized conditions	1812:1835	The optimized conditions of growth medium	1812:1852	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	12	7	theme	A.	1912:1913	arg1	MA1					1928:1930	A. senegalensis MA1	1912:1930	A. senegalensis MA1	1912:1930	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	5	8	theme	other	658:662	arg1	conditions					672:681	other culture conditions	658:681	other culture conditions	658:681	Besides, the production is also influenced by other culture conditions.					
32843009	4	9	attach	isolated	509:516	arg2	senengalensis					442:454	A. senengalensis	439:454	A. senengalensis	439:454	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	4	9	attach	isolated	509:516	arg1	fruits					529:534	Mango fruits	523:534	Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose	523:609	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	4	9	attach	isolated	509:516	arg2	coccus					501:506	a gram-negative, obligate aerobic, motile coccus	459:506	a gram-negative, obligate aerobic, motile coccus	459:506	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	12	10	theme	optimized	1816:1824	arg1	conditions					1826:1835	The optimized conditions	1812:1835	The optimized conditions of growth medium	1812:1852	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	13	11	theme	material	2138:2145	arg1	films					2166:2170	high-quality paper, wound dressing material, and nanocomposite films	2103:2170	high-quality paper, wound dressing material, and nanocomposite films	2103:2170	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	7	12	theme	incubation	1007:1016	arg1	time					1018:1021	incubation time	1007:1021	incubation time	1007:1021	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	9	13	theme	incubation	1364:1373	arg1	temperature					1375:1385	an incubation temperature	1361:1385	an incubation temperature of 33.5 °C. Around 20% of inoculum concentration	1361:1434	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	8	14	theme	Response	1156:1163	arg1	RSM					1186:1188	RSM	1186:1188	RSM	1186:1188	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	14	theme	Response	1156:1163	arg1	Methodology					1173:1183	Response Surface Methodology	1156:1183	Response Surface Methodology (RSM)	1156:1189	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	12	15	theme	20	1949:1950	arg1	times					1952:1956	times	1952:1956	times	1952:1956	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	1	16	from	plants	198:203	arg1	quantities					182:191	large quantities	176:191	large quantities from plants	176:203	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	1	17	theme	versatile	128:136	arg1	biomolecule					138:148	the most versatile biomolecule	119:148	the most versatile biomolecule on earth	119:157	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	1	17	theme	versatile	128:136	arg1	Cellulose					108:116	BACKGROUND Cellulose	97:116	BACKGROUND Cellulose	97:116	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	7	18	theme	inoculum	979:986	arg1	concentration					988:1000	inoculum concentration	979:1000	inoculum concentration	979:1000	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	9	19	theme	Maximum	1192:1198	arg1	production					1203:1212	Maximum BC production	1192:1212	Maximum BC production	1192:1212	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	2	20	theme	other	253:257	arg1	polymers					259:266	other polymers	253:266	other polymers like hemicellulose, lignin, and pectin	253:305	However, cellulose in plants is accompanied by other polymers like hemicellulose, lignin, and pectin.					
32843009	1	21	from	quantities	182:191	arg1	available					163:171	available	163:171	available	163:171	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	3	22	theme	other	315:319	arg1	hand					321:324	the other hand	311:324	the other hand	311:324	On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum.					
32843009	0	23	theme	Acetobacter	67:77	arg1	MA1					92:94	Acetobacter senegalensis MA1	67:94	Acetobacter senegalensis MA1	67:94	Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1.					
32843009	10	24	theme	33.5 °C	1704:1710	arg1	temperature					1689:1699	a temperature	1687:1699	a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000	1687:1745	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	13	25	theme	paper	2116:2120	arg1	films					2166:2170	high-quality paper, wound dressing material, and nanocomposite films	2103:2170	high-quality paper, wound dressing material, and nanocomposite films	2103:2170	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	10	26	from	pH 6.0	1663:1668	arg1	7.50 g L- 1					1631:1641	7.50 g L- 1	1631:1641	7.50 g L- 1 of yeast extract at pH 6.0	1631:1668	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	10	26	from	pH 6.0	1663:1668	arg1	50 mL L- 1					1607:1616	50 mL L- 1	1607:1616	50 mL L- 1 of glycerol	1607:1628	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	9	27	theme	inoculum	1413:1420	arg1	concentration					1422:1434	inoculum concentration	1413:1434	inoculum concentration	1413:1434	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	13	28	used	used	2055:2058	arg2	cellulose					2029:2037	the cellulose	2025:2037	the cellulose produced	2025:2046	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	11	29	theme	wet	1772:1774	arg1	weight					1776:1781	wet weight	1772:1781	wet weight	1772:1781	This gave a BC yield of wet weight as 469.83 g L- 1.					
32843009	9	30	theme	yeast	1242:1246	arg1	extract					1248:1254	yeast extract	1242:1254	yeast extract	1242:1254	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	1	31	from	biomolecule	138:148	arg1	earth					153:157	earth	153:157	earth	153:157	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	9	32	theme	high	1443:1446	arg1	yield					1448:1452	a high yield	1441:1452	a high yield after 30 days of inoculation	1441:1481	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	4	33	from	fruits	529:534	arg1	Senegal					539:545	Senegal	539:545	Senegal	539:545	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	4	34	dep	gram-negative	461:473	arg1	motile					494:499	motile	494:499	motile	494:499	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	4	34	dep	gram-negative	461:473	arg1	aerobic					485:491	aerobic	485:491	aerobic	485:491	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	12	35	dep	times	1952:1956	arg1	higher					1958:1963	higher	1958:1963	higher	1958:1963	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	9	36	theme	PEG	1261:1263	arg1	6000					1265:1268	PEG 6000	1261:1268	PEG 6000	1261:1268	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	10	37	theme	6000	1742:1745	arg1	7.76 g L- 1					1723:1733	7.76 g L- 1	1723:1733	7.76 g L- 1 of PEG 6000	1723:1745	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	3	38	theme	Acetobacter	418:428	arg1	producer					403:410	the most active producer	387:410	the most active producer	387:410	On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum.					
32843009	3	38	theme	Acetobacter	418:428	arg1	xylinum					430:436	Acetobacter xylinum	418:436	Acetobacter xylinum	418:436	On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum.					
32843009	8	39	theme	Methodology	1173:1183	arg1	Design					1140:1145	Central Composite Design	1122:1145	Central Composite Design (CCD) of Response Surface Methodology (RSM)	1122:1189	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	39	theme	Methodology	1173:1183	arg1	CCD					1148:1150	CCD	1148:1150	CCD	1148:1150	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	0	40	theme	bacterial	33:41	arg1	production					53:62	bacterial cellulose production	33:62	bacterial cellulose production by Acetobacter senegalensis MA1	33:94	Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1.					
32843009	7	41	dep	RESULTS	802:808	arg1	pre-optimized					871:883	pre-optimized	871:883	was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time	867:1021	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	9	42	theme	best	1277:1280	arg1	carbon					1282:1287	the best carbon	1273:1287	the best carbon	1273:1287	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	9	43	from	pH	1354:1355	arg1	polymer					1315:1321	polymer additive	1315:1330	polymer additive	1315:1330	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	10	44	theme	HS	1592:1593	arg1	medium					1595:1600	the HS medium	1588:1600	the HS medium	1588:1600	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	12	45	theme	cellulose	1899:1907	arg1	production					1875:1884	enhanced production	1866:1884	enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium	1866:2013	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	8	46	theme	Composite	1130:1138	arg1	Design					1140:1145	Central Composite Design	1122:1145	Central Composite Design (CCD) of Response Surface Methodology (RSM)	1122:1189	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	46	theme	Composite	1130:1138	arg1	CCD					1148:1150	CCD	1148:1150	CCD	1148:1150	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	7	47	theme	A.	846:847	arg1	MA1					863:865	A. senengalensis MA1	846:865	A. senengalensis MA1	846:865	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	8	48	theme	best	1083:1086	arg1	combination					1088:1098	the best combination	1079:1098	the best combination	1079:1098	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	6	49	dep	A.	723:724	arg1	senengalensis					726:738	senengalensis	726:738	senengalensis	726:738	Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced.					
32843009	6	50	theme	bacterial	767:775	arg1	BC					788:789	BC	788:789	BC	788:789	Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced.					
32843009	6	50	theme	bacterial	767:775	arg1	cellulose					777:785	the bacterial cellulose	763:785	the bacterial cellulose (BC) produced	763:799	Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced.					
32843009	10	51	theme	extract	1652:1658	arg1	7.50 g L- 1					1631:1641	7.50 g L- 1	1631:1641	7.50 g L- 1 of yeast extract at pH 6.0	1631:1668	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	10	51	theme	extract	1652:1658	arg1	50 mL L- 1					1607:1616	50 mL L- 1	1607:1616	50 mL L- 1 of glycerol	1607:1628	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	9	52	theme	additive	1323:1330	arg1	polymer					1315:1321	polymer additive	1315:1330	polymer additive	1315:1330	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	12	53	theme	enhanced	1866:1873	arg1	production					1875:1884	enhanced production	1866:1884	enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium	1866:2013	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	4	54	theme	capable	548:554	arg1	Senegal					539:545	Senegal	539:545	Senegal	539:545	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	7	55	theme	maximum	814:820	arg1	production					832:841	The maximum cellulose production	810:841	The maximum cellulose production by A. senengalensis MA1	810:865	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	12	56	theme	growth	1840:1845	arg1	medium					1847:1852	growth medium	1840:1852	growth medium	1840:1852	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	8	57	theme	Surface	1165:1171	arg1	RSM					1186:1188	RSM	1186:1188	RSM	1186:1188	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	57	theme	Surface	1165:1171	arg1	Methodology					1173:1183	Response Surface Methodology	1156:1183	Response Surface Methodology (RSM)	1156:1189	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	58	theme	Central	1122:1128	arg1	Design					1140:1145	Central Composite Design	1122:1145	Central Composite Design (CCD) of Response Surface Methodology (RSM)	1122:1189	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	58	theme	Central	1122:1128	arg1	CCD					1148:1150	CCD	1148:1150	CCD	1148:1150	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	9	59	from	temperature	1375:1385	arg1	polymer					1315:1321	polymer additive	1315:1330	polymer additive	1315:1330	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	12	60	theme	HS	2005:2006	arg1	medium					2008:2013	an unoptimized HS medium	1990:2013	an unoptimized HS medium	1990:2013	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	7	61	dep	A.	846:847	arg1	senengalensis					849:861	senengalensis	849:861	senengalensis	849:861	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	13	62	theme	nanocomposite	2152:2164	arg1	films					2166:2170	high-quality paper, wound dressing material, and nanocomposite films	2103:2170	high-quality paper, wound dressing material, and nanocomposite films	2103:2170	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	10	63	from	alterations	1554:1564	arg1	composition					1573:1583	the composition	1569:1583	the composition of the HS medium	1569:1600	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	13	64	theme	wound	2123:2127	arg1	material					2138:2145	wound dressing material	2123:2145	wound dressing material	2123:2145	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	4	65	theme	sugars	582:587	arg1	sugars					582:587	sugars	582:587	sugars	582:587	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	4	65	theme	sugars	582:587	arg1	variety					571:577	a variety	569:577	a variety of sugars	569:587	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	12	66	dep	CONCLUSION	1801:1810	arg1	resulted					1854:1861	resulted	1854:1861	resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium	1854:2013	CONCLUSION The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium.					
32843009	5	67	theme	culture	664:670	arg1	conditions					672:681	other culture conditions	658:681	other culture conditions	658:681	Besides, the production is also influenced by other culture conditions.					
32843009	10	68	theme	culture	1509:1515	arg1	conditions					1517:1526	culture conditions	1509:1526	culture conditions optimized by CCD	1509:1543	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	13	69	theme	dressing	2129:2136	arg1	material					2138:2145	wound dressing material	2123:2145	wound dressing material	2123:2145	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	9	70	theme	33.5 °C.	1390:1397	arg1	temperature					1375:1385	an incubation temperature	1361:1385	an incubation temperature of 33.5 °C. Around 20% of inoculum concentration	1361:1434	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	9	70	theme	33.5 °C.	1390:1397	arg1	pH					1354:1355	4.5 pH	1350:1355	4.5 pH	1350:1355	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	0	71	theme	senegalensis	79:90	arg1	MA1					92:94	Acetobacter senegalensis MA1	67:94	Acetobacter senegalensis MA1	67:94	Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1.					
32843009	9	72	theme	BC	1200:1201	arg1	production					1203:1212	Maximum BC production	1192:1212	Maximum BC production	1192:1212	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	7	73	theme	additive	952:959	arg1	polymer					944:950	polymer additive	944:959	polymer additive	944:959	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	13	74	theme	high-quality	2103:2114	arg1	paper					2116:2120	high-quality paper	2103:2120	high-quality paper	2103:2120	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	1	75	from	available	163:171	arg1	quantities					182:191	large quantities	176:191	large quantities from plants	176:203	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	9	76	theme	concentration	1422:1434	arg1	%					1408:1408	20%	1406:1408	20% of inoculum concentration	1406:1434	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	9	76	theme	concentration	1422:1434	arg1	concentration					1422:1434	inoculum concentration	1413:1434	inoculum concentration	1413:1434	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	2	77	from	cellulose	215:223	arg1	plants					228:233	plants	228:233	plants	228:233	However, cellulose in plants is accompanied by other polymers like hemicellulose, lignin, and pectin.					
32843009	3	78	theme	pure	327:330	arg1	cellulose					332:340	pure cellulose	327:340	pure cellulose	327:340	On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum.					
32843009	4	79	theme	gram-negative	461:473	arg1	senengalensis					442:454	A. senengalensis	439:454	A. senengalensis	439:454	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	4	79	theme	gram-negative	461:473	arg1	coccus					501:506	a gram-negative, obligate aerobic, motile coccus	459:506	a gram-negative, obligate aerobic, motile coccus	459:506	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	11	80	theme	weight	1776:1781	arg1	yield					1763:1767	a BC yield	1758:1767	a BC yield of wet weight	1758:1781	This gave a BC yield of wet weight as 469.83 g L- 1.					
32843009	0	81	theme	culture	10:16	arg1	conditions					18:27	culture conditions	10:27	culture conditions	10:27	Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1.					
32843009	1	82	theme	BACKGROUND	97:106	arg1	biomolecule					138:148	the most versatile biomolecule	119:148	the most versatile biomolecule on earth	119:157	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	1	82	theme	BACKGROUND	97:106	arg1	Cellulose					108:116	BACKGROUND Cellulose	97:116	BACKGROUND Cellulose	97:116	BACKGROUND Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants.					
32843009	4	83	theme	Mango	523:527	arg1	fruits					529:534	Mango fruits	523:534	Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose	523:609	A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose.					
32843009	3	84	theme	active	396:401	arg1	producer					403:410	the most active producer	387:410	the most active producer	387:410	On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum.					
32843009	3	84	theme	active	396:401	arg1	xylinum					430:436	Acetobacter xylinum	418:436	Acetobacter xylinum	418:436	On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum.					
32843009	7	85	theme	different	889:897	arg1	parameters					899:908	different parameters	889:908	different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time	889:1021	RESULTS The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time.					
32843009	0	86	theme	cellulose	43:51	arg1	production					53:62	bacterial cellulose production	33:62	bacterial cellulose production by Acetobacter senegalensis MA1	33:94	Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1.					
32843009	8	87	theme	pre-optimized	1037:1049	arg1	pooled					1067:1072	pooled	1067:1072	pooled	1067:1072	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	8	87	theme	pre-optimized	1037:1049	arg1	parameters					1051:1060	the pre-optimized parameters	1033:1060	the pre-optimized parameters	1033:1060	Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM).					
32843009	9	88	theme	inoculation	1471:1481	arg1	30 days					1460:1466	30 days	1460:1466	30 days of inoculation	1460:1481	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	10	89	theme	PEG	1738:1740	arg1	6000					1742:1745	PEG 6000	1738:1745	PEG 6000	1738:1745	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	6	90	theme	A.	723:724	arg1	MA1					740:742	A. senengalensis MA1	723:742	A. senengalensis MA1	723:742	Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced.					
32843009	10	91	theme	medium	1595:1600	arg1	composition					1573:1583	the composition	1569:1583	the composition of the HS medium	1569:1600	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	10	92	theme	glycerol	1621:1628	arg1	7.50 g L- 1					1631:1641	7.50 g L- 1	1631:1641	7.50 g L- 1 of yeast extract at pH 6.0	1631:1668	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	10	92	theme	glycerol	1621:1628	arg1	50 mL L- 1					1607:1616	50 mL L- 1	1607:1616	50 mL L- 1 of glycerol	1607:1628	The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L- 1 of glycerol, 7.50 g L- 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L- 1 of PEG 6000.					
32843009	9	93	theme	nitrogen	1293:1300	arg1	sources					1302:1308	nitrogen sources	1293:1308	nitrogen sources	1293:1308	Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation.					
32843009	13	94	theme	food	2176:2179	arg1	packaging					2181:2189	food packaging	2176:2189	food packaging	2176:2189	Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.					
32843009	11	95	theme	BC	1760:1761	arg1	yield					1763:1767	a BC yield	1758:1767	a BC yield of wet weight	1758:1781	This gave a BC yield of wet weight as 469.83 g L- 1.					
32890396	5	0	gly	glycoprotein	847:858	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	0	gly	glycoprotein	847:858	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	2	1	theme	proteins	324:331	arg1	structures					299:308	high resolution structures	283:308	high resolution structures of SARS-CoV-2 proteins	283:331	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	5	2	theme	neutralizing	890:901	arg1	antibodies					903:912	neutralizing antibodies	890:912	neutralizing antibodies	890:912	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	4	3	with	users	632:636	arg1	antibodies					725:734	antibodies	725:734	antibodies	725:734	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	3	with	users	632:636	arg1	structures					665:674	structures	665:674	structures of coronavirus proteins	665:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	3	with	users	632:636	arg1	sets					657:660	comprehensive sets	643:660	comprehensive sets of structures of coronavirus proteins	643:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	3	with	users	632:636	arg1	molecules					758:766	small molecules	752:766	small molecules	752:766	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	3	with	users	632:636	arg1	receptors					737:745	receptors	737:745	receptors	737:745	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	6	4	theme	spike	1279:1283	arg1	conformations					1285:1297	spike conformations	1279:1297	spike conformations	1279:1297	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	5	5	theme	major	874:878	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	5	theme	major	874:878	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	2	6	theme	SARS-CoV-2	313:322	arg1	proteins					324:331	SARS-CoV-2 proteins	313:331	SARS-CoV-2 proteins	313:331	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	6	7	theme	conformations	1285:1297	arg1	classification					1261:1274	structure-based classification	1245:1274	structure-based classification of spike conformations, generated by unsupervised clustering	1245:1335	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	8	theme	modeled	1185:1191	arg1	N-glycosylation					1193:1207	modeled N-glycosylation	1185:1207	modeled N-glycosylation at known glycan sites	1185:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	5	9	theme	variability	1002:1012	arg1	complexes					976:984	spike-antibody complexes	961:984	spike-antibody complexes	961:984	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	9	theme	variability	1002:1012	arg1	structures					823:832	structures	823:832	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	823:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	9	theme	variability	1002:1012	arg1	polymorphisms					1025:1037	known polymorphisms	1019:1037	known polymorphisms	1019:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	9	theme	variability	1002:1012	arg1	sets					953:956	sets	953:956	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	953:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	9	theme	variability	1002:1012	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	9	theme	variability	1002:1012	arg1	variability					1002:1012	spike sequence variability	987:1012	spike sequence variability	987:1012	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	9	theme	variability	1002:1012	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	10	theme	Integrated	769:778	arg1	viewers					790:796	Integrated molecular viewers	769:796	Integrated molecular viewers	769:796	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	7	11	theme	protein	1449:1455	arg1	structures					1457:1466	other coronavirus protein structures	1431:1466	other coronavirus protein structures	1431:1466	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	2	12	theme	rapid	255:259	arg1	rise					261:264	a rapid rise	253:264	a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities	253:387	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	3	13	dep	database	523:530	arg1	a					521:521	a	521:521	a	521:521	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	4	14	theme	proteins	691:698	arg1	structures					665:674	structures	665:674	structures of coronavirus proteins	665:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	6	15	theme	structure-based	1245:1259	arg1	classification					1261:1274	structure-based classification	1245:1274	structure-based classification of spike conformations, generated by unsupervised clustering	1245:1335	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	4	16	theme	small	752:756	arg1	molecules					758:766	small molecules	752:766	small molecules	752:766	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	6	17	from	sites	1225:1229	arg1	N-glycosylation					1193:1207	modeled N-glycosylation	1185:1207	modeled N-glycosylation at known glycan sites	1185:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	1	18	theme	global	148:153	arg1	health					155:160	global health	148:160	global health posed by coronaviruses	148:183	SARS-CoV-2, the etiologic agent of COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32890396	5	19	theme	molecular	780:788	arg1	viewers					790:796	Integrated molecular viewers	769:796	Integrated molecular viewers	769:796	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	6	20	gly	glycoprotein	1105:1116	arg1	glycoprotein					1105:1116	the spike glycoprotein	1095:1116	the spike glycoprotein	1095:1116	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	7	21	theme	other	1431:1435	arg1	structures					1457:1466	other coronavirus protein structures	1431:1466	other coronavirus protein structures	1431:1466	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	6	22	theme	structure-based	1056:1070	arg1	design					1072:1077	structure-based design	1056:1077	structure-based design	1056:1077	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	5	23	theme	glycoprotein	847:858	arg1	complexes					976:984	spike-antibody complexes	961:984	spike-antibody complexes	961:984	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	23	theme	glycoprotein	847:858	arg1	structures					823:832	structures	823:832	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	823:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	23	theme	glycoprotein	847:858	arg1	polymorphisms					1025:1037	known polymorphisms	1019:1037	known polymorphisms	1019:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	23	theme	glycoprotein	847:858	arg1	sets					953:956	sets	953:956	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	953:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	23	theme	glycoprotein	847:858	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	23	theme	glycoprotein	847:858	arg1	variability					1002:1012	spike sequence variability	987:1012	spike sequence variability	987:1012	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	23	theme	glycoprotein	847:858	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	24	theme	vaccine	918:924	arg1	efforts					933:939	vaccine design efforts	918:939	vaccine design efforts	918:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	4	25	theme	comprehensive	643:655	arg1	structures					665:674	structures	665:674	structures of coronavirus proteins	665:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	25	theme	comprehensive	643:655	arg1	sets					657:660	comprehensive sets	643:660	comprehensive sets of structures of coronavirus proteins	643:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	7	26	theme	coronavirus	1437:1447	arg1	structures					1457:1466	other coronavirus protein structures	1431:1466	other coronavirus protein structures	1431:1466	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	6	27	theme	glycoprotein	1105:1116	arg1	analysis					1083:1090	analysis	1083:1090	analysis of the spike glycoprotein	1083:1116	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	27	theme	glycoprotein	1105:1116	arg1	design					1072:1077	structure-based design	1056:1077	structure-based design	1056:1077	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	3	28	theme	coronavirus	549:559	arg1	structures					569:578	coronavirus protein structures	549:578	coronavirus protein structures	549:578	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	3	29	theme	structure-based	400:414	arg1	design					416:421	structure-based design	400:421	structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses	400:493	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	1	30	theme	etiologic	85:93	arg1	SARS-CoV-2					69:78	SARS-CoV-2	69:78	SARS-CoV-2	69:78	SARS-CoV-2, the etiologic agent of COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32890396	1	30	theme	etiologic	85:93	arg1	agent					95:99	the etiologic agent	81:99	the etiologic agent of COVID-19	81:111	SARS-CoV-2, the etiologic agent of COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32890396	7	31	theme	centralized	1382:1392	arg1	community					1367:1375	the research community	1354:1375	the research community	1354:1375	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	7	31	theme	centralized	1382:1392	arg1	CoV3D					1338:1342	CoV3D	1338:1342	CoV3D	1338:1342	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	7	31	theme	centralized	1382:1392	arg1	reference					1394:1402	a centralized reference	1380:1402	a centralized reference	1380:1402	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	3	32	theme	therapeutics	439:450	arg1	design					416:421	structure-based design	400:421	structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses	400:493	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	5	33	theme	design	926:931	arg1	efforts					933:939	vaccine design efforts	918:939	vaccine design efforts	918:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	6	34	theme	spike	1163:1167	arg1	structures					1169:1178	spike structures	1163:1178	spike structures with modeled N-glycosylation at known glycan sites	1163:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	4	35	theme	structures	665:674	arg1	structures					665:674	structures	665:674	structures of coronavirus proteins	665:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	35	theme	structures	665:674	arg1	sets					657:660	comprehensive sets	643:660	comprehensive sets of structures of coronavirus proteins	643:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	6	36	contain	contains	1236:1243	arg1	CoV3D					1119:1123	CoV3D	1119:1123	CoV3D	1119:1123	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	36	contain	contains	1236:1243	arg2	classification					1261:1274	structure-based classification	1245:1274	structure-based classification of spike conformations, generated by unsupervised clustering	1245:1335	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	0	37	theme	high	21:24	arg1	structures					57:66	high resolution coronavirus protein structures	21:66	high resolution coronavirus protein structures	21:66	CoV3D: a database of high resolution coronavirus protein structures.					
32890396	2	38	theme	structures	299:308	arg1	number					273:278	the number	269:278	the number of high resolution structures of SARS-CoV-2 proteins	269:331	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	6	39	theme	known	1212:1216	arg1	sites					1225:1229	known glycan sites	1212:1229	known glycan sites	1212:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	3	40	theme	vaccines	426:433	arg1	design					416:421	structure-based design	400:421	structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses	400:493	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	5	41	theme	spike	987:991	arg1	variability					1002:1012	spike sequence variability	987:1012	spike sequence variability	987:1012	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	6	42	theme	structures	1169:1178	arg1	download					1151:1158	download	1151:1158	download	1151:1158	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	42	theme	structures	1169:1178	arg1	visualization					1133:1145	visualization	1133:1145	visualization	1133:1145	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	2	43	theme	vulnerabilities	373:387	arg1	map					360:362	a map	358:362	a map of virus vulnerabilities	358:387	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	5	44	theme	spike	841:845	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	44	theme	spike	841:845	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	6	45	with	structures	1169:1178	arg1	N-glycosylation					1193:1207	modeled N-glycosylation	1185:1207	modeled N-glycosylation at known glycan sites	1185:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	3	46	theme	weekly	603:608	arg1	basis					610:614	a weekly basis	601:614	a weekly basis	601:614	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	2	47	theme	effective	206:214	arg1	vaccines					216:223	effective vaccines	206:223	effective vaccines	206:223	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	2	48	theme	virus	367:371	arg1	vulnerabilities					373:387	virus vulnerabilities	367:387	virus vulnerabilities	367:387	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	0	49	theme	coronavirus	37:47	arg1	structures					57:66	high resolution coronavirus protein structures	21:66	high resolution coronavirus protein structures	21:66	CoV3D: a database of high resolution coronavirus protein structures.					
32890396	6	50	theme	unsupervised	1313:1324	arg1	clustering					1326:1335	unsupervised clustering	1313:1335	unsupervised clustering	1313:1335	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	51	gly	N-glycosylation	1193:1207	arg2	sites					1225:1229	known glycan sites	1212:1229	known glycan sites	1212:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	51	gly	N-glycosylation	1193:1207	arg1	structures					1169:1178	spike structures	1163:1178	spike structures with modeled N-glycosylation at known glycan sites	1163:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	6	51	gly	N-glycosylation	1193:1207	arg1	sites					1225:1229	known glycan sites	1212:1229	known glycan sites	1212:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	3	52	theme	protein	561:567	arg1	structures					569:578	coronavirus protein structures	549:578	coronavirus protein structures	549:578	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	6	53	theme	spike	1099:1103	arg1	glycoprotein					1105:1116	the spike glycoprotein	1095:1116	the spike glycoprotein	1095:1116	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	3	54	theme	other	475:479	arg1	coronaviruses					481:493	other coronaviruses	475:493	other coronaviruses	475:493	To assist structure-based design of vaccines and therapeutics against SARS-CoV-2 and other coronaviruses, we have developed CoV3D, a database and resource for coronavirus protein structures, which is updated on a weekly basis.					
32890396	0	55	theme	resolution	26:35	arg1	structures					57:66	high resolution coronavirus protein structures	21:66	high resolution coronavirus protein structures	21:66	CoV3D: a database of high resolution coronavirus protein structures.					
32890396	6	56	theme	glycan	1218:1223	arg1	sites					1225:1229	known glycan sites	1212:1229	known glycan sites	1212:1229	In order to aid structure-based design and analysis of the spike glycoprotein, CoV3D permits visualization and download of spike structures with modeled N-glycosylation at known glycan sites, and contains structure-based classification of spike conformations, generated by unsupervised clustering.					
32890396	2	57	theme	urgent	190:195	arg1	need					197:200	The urgent need	186:200	The urgent need for effective vaccines and therapies	186:237	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	1	58	theme	COVID-19	104:111	arg1	SARS-CoV-2					69:78	SARS-CoV-2	69:78	SARS-CoV-2	69:78	SARS-CoV-2, the etiologic agent of COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32890396	1	58	theme	COVID-19	104:111	arg1	agent					95:99	the etiologic agent	81:99	the etiologic agent of COVID-19	81:111	SARS-CoV-2, the etiologic agent of COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32890396	4	59	with	complexes	710:718	arg1	antibodies					725:734	antibodies	725:734	antibodies	725:734	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	59	with	complexes	710:718	arg1	structures					665:674	structures	665:674	structures of coronavirus proteins	665:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	59	with	complexes	710:718	arg1	molecules					758:766	small molecules	752:766	small molecules	752:766	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	59	with	complexes	710:718	arg1	sets					657:660	comprehensive sets	643:660	comprehensive sets of structures of coronavirus proteins	643:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	4	59	with	complexes	710:718	arg1	receptors					737:745	receptors	737:745	receptors	737:745	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
32890396	5	60	theme	known	1019:1023	arg1	polymorphisms					1025:1037	known polymorphisms	1019:1037	known polymorphisms	1019:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	7	61	theme	research	1358:1365	arg1	community					1367:1375	the research community	1354:1375	the research community	1354:1375	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	7	61	theme	research	1358:1365	arg1	CoV3D					1338:1342	CoV3D	1338:1342	CoV3D	1338:1342	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	7	61	theme	research	1358:1365	arg1	reference					1394:1402	a centralized reference	1380:1402	a centralized reference	1380:1402	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	5	62	theme	sequence	993:1000	arg1	variability					1002:1012	spike sequence variability	987:1012	spike sequence variability	987:1012	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	2	63	theme	resolution	288:297	arg1	structures					299:308	high resolution structures	283:308	high resolution structures of SARS-CoV-2 proteins	283:331	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	5	64	theme	efforts	933:939	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	64	theme	efforts	933:939	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	complexes					976:984	spike-antibody complexes	961:984	spike-antibody complexes	961:984	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	structures					823:832	structures	823:832	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	823:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	polymorphisms					1025:1037	known polymorphisms	1019:1037	known polymorphisms	1019:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	sets					953:956	sets	953:956	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	953:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	variability					1002:1012	spike sequence variability	987:1012	spike sequence variability	987:1012	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	65	theme	polymorphisms	1025:1037	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	66	theme	spike-antibody	961:974	arg1	complexes					976:984	spike-antibody complexes	961:984	spike-antibody complexes	961:984	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	2	67	theme	high	283:286	arg1	structures					299:308	high resolution structures	283:308	high resolution structures of SARS-CoV-2 proteins	283:331	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	2	68	from	rise	261:264	arg1	number					273:278	the number	269:278	the number of high resolution structures of SARS-CoV-2 proteins	269:331	The urgent need for effective vaccines and therapies is leading to a rapid rise in the number of high resolution structures of SARS-CoV-2 proteins that collectively reveal a map of virus vulnerabilities.					
32890396	5	69	theme	antibodies	903:912	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	69	theme	antibodies	903:912	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	0	70	theme	structures	57:66	arg1	database					9:16	a database	7:16	CoV3D: a database of high resolution coronavirus protein structures.	0:67	CoV3D: a database of high resolution coronavirus protein structures.					
32890396	5	71	theme	complexes	976:984	arg1	complexes					976:984	spike-antibody complexes	961:984	spike-antibody complexes	961:984	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	71	theme	complexes	976:984	arg1	structures					823:832	structures	823:832	structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts	823:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	71	theme	complexes	976:984	arg1	polymorphisms					1025:1037	known polymorphisms	1019:1037	known polymorphisms	1019:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	71	theme	complexes	976:984	arg1	sets					953:956	sets	953:956	sets of spike-antibody complexes, spike sequence variability, and known polymorphisms	953:1037	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	71	theme	complexes	976:984	arg1	target					880:885	the major target	870:885	the major target of neutralizing antibodies and vaccine design efforts	870:939	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	71	theme	complexes	976:984	arg1	variability					1002:1012	spike sequence variability	987:1012	spike sequence variability	987:1012	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	5	71	theme	complexes	976:984	arg1	glycoprotein					847:858	the spike glycoprotein	837:858	the spike glycoprotein	837:858	Integrated molecular viewers allow users to visualize structures of the spike glycoprotein, which is the major target of neutralizing antibodies and vaccine design efforts, as well as sets of spike-antibody complexes, spike sequence variability, and known polymorphisms.					
32890396	7	72	dep	serve	1348:1352	arg1	//cov3d.ibbr.umd.edu					1496:1515	//cov3d.ibbr.umd.edu	1496:1515	//cov3d.ibbr.umd.edu	1496:1515	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	7	72	dep	serve	1348:1352	arg1	https					1490:1494	https	1490:1494	https	1490:1494	CoV3D can serve the research community as a centralized reference and resource for spike and other coronavirus protein structures, and is available at: https://cov3d.ibbr.umd.edu.					
32890396	0	73	theme	protein	49:55	arg1	structures					57:66	high resolution coronavirus protein structures	21:66	high resolution coronavirus protein structures	21:66	CoV3D: a database of high resolution coronavirus protein structures.					
32890396	0	74	dep	CoV3D	0:4	arg1	database					9:16	a database	7:16	CoV3D: a database of high resolution coronavirus protein structures.	0:67	CoV3D: a database of high resolution coronavirus protein structures.					
32890396	1	75	theme	general	130:136	arg1	threat					138:143	the general threat	126:143	the general threat to global health posed by coronaviruses	126:183	SARS-CoV-2, the etiologic agent of COVID-19, exemplifies the general threat to global health posed by coronaviruses.					
32890396	4	76	theme	coronavirus	679:689	arg1	proteins					691:698	coronavirus proteins	679:698	coronavirus proteins	679:698	CoV3D provides users with comprehensive sets of structures of coronavirus proteins and their complexes with antibodies, receptors, and small molecules.					
34254733	0	0	theme	engineering	95:105	arg1	applications					107:118	heart valve tissue engineering applications	76:118	heart valve tissue engineering applications	76:118	Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.					
34254733	6	1	theme	cell	977:980	arg1	phenotypes					982:991	valve interstitial cell phenotypes	958:991	valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media	958:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	0	2	theme	tissue	88:93	arg1	applications					107:118	heart valve tissue engineering applications	76:118	heart valve tissue engineering applications	76:118	Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.					
34254733	6	3	theme	interstitial	964:975	arg1	phenotypes					982:991	valve interstitial cell phenotypes	958:991	valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media	958:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	4	theme	stem	882:885	arg1	HADMSC					894:899	HADMSC	894:899	HADMSC	894:899	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	4	theme	stem	882:885	arg1	cells					887:891	Human adipose-derived mesenchymal stem cells	848:891	Human adipose-derived mesenchymal stem cells (HADMSC)	848:900	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	2	5	theme	fibroblast	382:391	arg1	phenotype					393:401	quiescent fibroblast phenotype	372:401	quiescent fibroblast phenotype	372:401	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	9	6	theme	enhancing	1555:1563	arg1	agent					1565:1569	a viscosity enhancing agent	1543:1569	a viscosity enhancing agent	1543:1569	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	4	7	theme	mNCC-MeGel	630:639	arg1	series					581:586	a series	579:586	a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG)	579:645	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	6	8	theme	valve	958:962	arg1	phenotypes					982:991	valve interstitial cell phenotypes	958:991	valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media	958:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	9	theme	mesenchymal	870:880	arg1	HADMSC					894:899	HADMSC	894:899	HADMSC	894:899	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	9	theme	mesenchymal	870:880	arg1	cells					887:891	Human adipose-derived mesenchymal stem cells	848:891	Human adipose-derived mesenchymal stem cells (HADMSC)	848:900	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	7	10	theme	quiescent	1267:1275	arg1	phenotype					1290:1298	a quiescent fibroblastic phenotype	1265:1298	a quiescent fibroblastic phenotype	1265:1298	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	2	11	theme	nonlinear	313:321	arg1	properties					352:361	nonlinear elastic tissue biomechanical properties	313:361	nonlinear elastic tissue biomechanical properties	313:361	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	9	12	theme	principle	1500:1508	arg1	hydrogel					1519:1526	the mNG hydrogel	1511:1526	the mNG hydrogel	1511:1526	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	9	12	theme	principle	1500:1508	arg1	proof					1491:1495	a proof	1489:1495	a proof of principle	1489:1508	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	9	13	theme	tubular	1618:1624	arg1	structure					1626:1634	a tall, self-standing tubular structure	1596:1634	a tall, self-standing tubular structure that sustained cell viability	1596:1664	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	7	14	theme	fibroblastic	1277:1288	arg1	phenotype					1290:1298	a quiescent fibroblastic phenotype	1265:1298	a quiescent fibroblastic phenotype	1265:1298	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	6	15	theme	adipose-derived	854:868	arg1	HADMSC					894:899	HADMSC	894:899	HADMSC	894:899	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	15	theme	adipose-derived	854:868	arg1	cells					887:891	Human adipose-derived mesenchymal stem cells	848:891	Human adipose-derived mesenchymal stem cells (HADMSC)	848:900	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	9	16	theme	tall	1598:1601	arg1	structure					1626:1634	a tall, self-standing tubular structure	1596:1634	a tall, self-standing tubular structure that sustained cell viability	1596:1664	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	9	17	dep	tall	1598:1601	arg1	self-standing					1604:1616	self-standing	1604:1616	self-standing	1604:1616	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	2	18	theme	quiescent	372:380	arg1	phenotype					393:401	quiescent fibroblast phenotype	372:401	quiescent fibroblast phenotype	372:401	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	7	19	theme	muscle	1153:1158	arg1	αSMA					1167:1170	αSMA	1167:1170	αSMA	1167:1170	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	7	19	theme	muscle	1153:1158	arg1	actin					1160:1164	alpha smooth muscle actin	1140:1164	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	6	20	theme	Human	848:852	arg1	HADMSC					894:899	HADMSC	894:899	HADMSC	894:899	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	20	theme	Human	848:852	arg1	cells					887:891	Human adipose-derived mesenchymal stem cells	848:891	Human adipose-derived mesenchymal stem cells (HADMSC)	848:900	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	3	21	theme	tunable	474:480	arg1	material					493:500	an attractive tunable biological material	460:500	an attractive tunable biological material that has not been employed to this application	460:547	Nanocellulose is an attractive tunable biological material that has not been employed to this application.					
34254733	3	21	theme	tunable	474:480	arg1	Nanocellulose					443:455	Nanocellulose	443:455	Nanocellulose	443:455	Nanocellulose is an attractive tunable biological material that has not been employed to this application.					
34254733	8	22	theme	osteogenic	1307:1316	arg1	conditions					1324:1333	osteogenic media conditions	1307:1333	osteogenic media conditions	1307:1333	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	5	23	theme	uniaxial	818:825	arg1	testing					839:845	uniaxial compression testing	818:845	uniaxial compression testing	818:845	Their structures were characterized by FTIR, 1 HNMR and uniaxial compression testing.					
34254733	2	24	theme	valve	285:289	arg1	leaflets					291:298	engineered valve leaflets	274:298	engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation	274:440	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	1	25	theme	elusive	183:189	arg1	goal					191:194	an elusive goal	180:194	an elusive goal for nearly 30 years	180:214	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	25	theme	elusive	183:189	arg1	valves					156:161	Functional tissue engineered heart valves	121:161	Functional tissue engineered heart valves (TEHV)	121:168	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	0	26	theme	nanocrystalline	14:28	arg1	cellulose					30:38	nanocrystalline cellulose	14:38	nanocrystalline cellulose	14:38	Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.					
34254733	4	27	theme	composite	610:618	arg1	mNG					642:644	mNG	642:644	mNG	642:644	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	27	theme	composite	610:618	arg1	mNCC-MeGel					630:639	photocrosslinkable composite hydrogels mNCC-MeGel	591:639	photocrosslinkable composite hydrogels mNCC-MeGel (mNG)	591:645	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	7	28	theme	alpha	1140:1144	arg1	αSMA					1167:1170	αSMA	1167:1170	αSMA	1167:1170	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	7	28	theme	alpha	1140:1144	arg1	actin					1160:1164	alpha smooth muscle actin	1140:1164	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	6	29	link	adipose-derived	854:868	arg1	HADMSC					894:899	HADMSC	894:899	HADMSC	894:899	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	6	29	link	adipose-derived	854:868	arg1	cells					887:891	Human adipose-derived mesenchymal stem cells	848:891	Human adipose-derived mesenchymal stem cells (HADMSC)	848:900	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	2	30	theme	biomechanical	338:350	arg1	properties					352:361	nonlinear elastic tissue biomechanical properties	313:361	nonlinear elastic tissue biomechanical properties	313:361	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	2	31	theme	osteogenic	415:424	arg1	differentiation					426:440	osteogenic differentiation	415:440	osteogenic differentiation	415:440	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	4	32	theme	photocrosslinkable	591:608	arg1	mNG					642:644	mNG	642:644	mNG	642:644	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	32	theme	photocrosslinkable	591:608	arg1	mNCC-MeGel					630:639	photocrosslinkable composite hydrogels mNCC-MeGel	591:639	photocrosslinkable composite hydrogels mNCC-MeGel (mNG)	591:645	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	7	33	theme	vimentin	1198:1205	arg1	expression					1220:1229	increased vimentin and aggrecan expression	1188:1229	increased vimentin and aggrecan expression	1188:1229	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	2	34	theme	tissue	331:336	arg1	properties					352:361	nonlinear elastic tissue biomechanical properties	313:361	nonlinear elastic tissue biomechanical properties	313:361	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	3	35	theme	biological	482:491	arg1	material					493:500	an attractive tunable biological material	460:500	an attractive tunable biological material that has not been employed to this application	460:547	Nanocellulose is an attractive tunable biological material that has not been employed to this application.					
34254733	3	35	theme	biological	482:491	arg1	Nanocellulose					443:455	Nanocellulose	443:455	Nanocellulose	443:455	Nanocellulose is an attractive tunable biological material that has not been employed to this application.					
34254733	7	36	theme	aggrecan	1211:1218	arg1	expression					1220:1229	increased vimentin and aggrecan expression	1188:1229	increased vimentin and aggrecan expression	1188:1229	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	10	37	theme	attractive	1710:1719	arg1	mNG					1700:1702	mNG	1700:1702	mNG	1700:1702	Together, these results identify mNG as an attractive biomaterial for TEHV applications.					
34254733	10	37	theme	attractive	1710:1719	arg1	biomaterial					1721:1731	an attractive biomaterial	1707:1731	an attractive biomaterial for TEHV applications	1707:1753	Together, these results identify mNG as an attractive biomaterial for TEHV applications.					
34254733	6	38	theme	normal	1022:1027	arg1	media					1044:1048	both normal and osteogenic media	1017:1048	both normal and osteogenic media	1017:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	2	39	theme	elastic	323:329	arg1	properties					352:361	nonlinear elastic tissue biomechanical properties	313:361	nonlinear elastic tissue biomechanical properties	313:361	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	4	40	theme	hydrogels	620:628	arg1	mNG					642:644	mNG	642:644	mNG	642:644	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	40	theme	hydrogels	620:628	arg1	mNCC-MeGel					630:639	photocrosslinkable composite hydrogels mNCC-MeGel	591:639	photocrosslinkable composite hydrogels mNCC-MeGel (mNG)	591:645	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	7	41	theme	decreased	1130:1138	arg1	expression					1173:1182	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	1	42	theme	tissue	132:137	arg1	valves					156:161	Functional tissue engineered heart valves	121:161	Functional tissue engineered heart valves (TEHV)	121:168	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	42	theme	tissue	132:137	arg1	goal					191:194	an elusive goal	180:194	an elusive goal for nearly 30 years	180:214	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	42	theme	tissue	132:137	arg1	TEHV					164:167	TEHV	164:167	TEHV	164:167	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	8	43	theme	osteogenic	1391:1400	arg1	osteocalcin					1429:1439	osteocalcin	1429:1439	osteocalcin	1429:1439	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	8	43	theme	osteogenic	1391:1400	arg1	genes					1402:1406	osteogenic genes	1391:1406	osteogenic genes	1391:1406	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	8	43	theme	osteogenic	1391:1400	arg1	Runx2					1419:1423	Runx2	1419:1423	Runx2	1419:1423	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	0	44	theme	multifunctional	47:61	arg1	hydrogel					63:70	a multifunctional hydrogel	45:70	a multifunctional hydrogel for heart valve tissue engineering applications	45:118	Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.					
34254733	2	45	contain	possess	305:311	arg2	properties					352:361	nonlinear elastic tissue biomechanical properties	313:361	nonlinear elastic tissue biomechanical properties	313:361	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	2	45	contain	possess	305:311	arg1	leaflets					291:298	engineered valve leaflets	274:298	engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation	274:440	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	9	46	theme	cell	1651:1654	arg1	viability					1656:1664	cell viability	1651:1664	cell viability	1651:1664	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	8	47	theme	media	1318:1322	arg1	conditions					1324:1333	osteogenic media conditions	1307:1333	osteogenic media conditions	1307:1333	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	8	48	theme	mNG	1350:1352	arg1	hydrogels					1354:1362	mNG hydrogels	1350:1362	mNG hydrogels	1350:1362	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	3	49	theme	attractive	463:472	arg1	material					493:500	an attractive tunable biological material	460:500	an attractive tunable biological material that has not been employed to this application	460:547	Nanocellulose is an attractive tunable biological material that has not been employed to this application.					
34254733	3	49	theme	attractive	463:472	arg1	Nanocellulose					443:455	Nanocellulose	443:455	Nanocellulose	443:455	Nanocellulose is an attractive tunable biological material that has not been employed to this application.					
34254733	10	50	theme	TEHV	1737:1740	arg1	applications					1742:1753	TEHV applications	1737:1753	TEHV applications	1737:1753	Together, these results identify mNG as an attractive biomaterial for TEHV applications.					
34254733	5	51	theme	compression	827:837	arg1	testing					839:845	uniaxial compression testing	818:845	uniaxial compression testing	818:845	Their structures were characterized by FTIR, 1 HNMR and uniaxial compression testing.					
34254733	7	52	theme	control	1073:1079	arg1	group					1081:1085	the MeGel control group	1063:1085	the MeGel control group	1063:1085	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	9	53	theme	viscosity	1545:1553	arg1	agent					1565:1569	a viscosity enhancing agent	1543:1569	a viscosity enhancing agent	1543:1569	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	9	54	theme	mNG	1515:1517	arg1	hydrogel					1519:1526	the mNG hydrogel	1511:1526	the mNG hydrogel	1511:1526	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	9	54	theme	mNG	1515:1517	arg1	proof					1491:1495	a proof	1489:1495	a proof of principle	1489:1508	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	2	55	theme	persistent	227:236	arg1	challenges					238:247	the persistent challenges	223:247	the persistent challenges	223:247	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	1	56	theme	engineered	139:148	arg1	valves					156:161	Functional tissue engineered heart valves	121:161	Functional tissue engineered heart valves (TEHV)	121:168	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	56	theme	engineered	139:148	arg1	goal					191:194	an elusive goal	180:194	an elusive goal for nearly 30 years	180:214	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	56	theme	engineered	139:148	arg1	TEHV					164:167	TEHV	164:167	TEHV	164:167	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	7	57	theme	increased	1188:1196	arg1	expression					1220:1229	increased vimentin and aggrecan expression	1188:1229	increased vimentin and aggrecan expression	1188:1229	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	6	58	theme	14 days	998:1004	arg1	culture					1006:1012	14 days culture	998:1012	14 days culture in both normal and osteogenic media	998:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	8	59	theme	genes	1402:1406	arg1	expression					1377:1386	lower expression	1371:1386	lower expression of osteogenic genes, including Runx2 and osteocalcin	1371:1439	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	7	60	theme	actin	1160:1164	arg1	expression					1173:1182	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	1	61	theme	heart	150:154	arg1	valves					156:161	Functional tissue engineered heart valves	121:161	Functional tissue engineered heart valves (TEHV)	121:168	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	61	theme	heart	150:154	arg1	goal					191:194	an elusive goal	180:194	an elusive goal for nearly 30 years	180:214	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	61	theme	heart	150:154	arg1	TEHV					164:167	TEHV	164:167	TEHV	164:167	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	2	62	theme	engineered	274:283	arg1	leaflets					291:298	engineered valve leaflets	274:298	engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation	274:440	Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation.					
34254733	7	63	theme	MeGel	1067:1071	arg1	group					1081:1085	the MeGel control group	1063:1085	the MeGel control group	1063:1085	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	1	64	theme	Functional	121:130	arg1	valves					156:161	Functional tissue engineered heart valves	121:161	Functional tissue engineered heart valves (TEHV)	121:168	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	64	theme	Functional	121:130	arg1	goal					191:194	an elusive goal	180:194	an elusive goal for nearly 30 years	180:214	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	1	64	theme	Functional	121:130	arg1	TEHV					164:167	TEHV	164:167	TEHV	164:167	Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years.					
34254733	6	65	from	culture	1006:1012	arg1	media					1044:1048	both normal and osteogenic media	1017:1048	both normal and osteogenic media	1017:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	8	66	theme	lower	1371:1375	arg1	expression					1377:1386	lower expression	1371:1386	lower expression of osteogenic genes, including Runx2 and osteocalcin	1371:1439	Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance toward calcification.					
34254733	7	67	theme	smooth	1146:1151	arg1	αSMA					1167:1170	αSMA	1167:1170	αSMA	1167:1170	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	7	67	theme	smooth	1146:1151	arg1	actin					1160:1164	alpha smooth muscle actin	1140:1164	decreased alpha smooth muscle actin (αSMA) expression	1130:1182	Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype.					
34254733	0	68	theme	valve	82:86	arg1	applications					107:118	heart valve tissue engineering applications	76:118	heart valve tissue engineering applications	76:118	Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.					
34254733	4	69	theme	gelatin	745:751	arg1	backbone					719:726	the backbone	715:726	the backbone of methacrylated gelatin (MeGel)	715:759	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	9	70	used	used	1576:1579	arg2	proof					1491:1495	a proof	1489:1495	a proof of principle	1489:1508	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	9	70	used	used	1576:1579	arg2	hydrogel					1519:1526	the mNG hydrogel	1511:1526	the mNG hydrogel	1511:1526	As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability.					
34254733	4	71	theme	nanocrystalline	677:691	arg1	mNCC					704:707	mNCC	704:707	mNCC	704:707	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	71	theme	nanocrystalline	677:691	arg1	cellulose					693:701	TEMPO-modified nanocrystalline cellulose	662:701	TEMPO-modified nanocrystalline cellulose (mNCC)	662:708	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	0	72	theme	heart	76:80	arg1	applications					107:118	heart valve tissue engineering applications	76:118	heart valve tissue engineering applications	76:118	Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.					
34254733	6	73	theme	osteogenic	1033:1042	arg1	media					1044:1048	both normal and osteogenic media	1017:1048	both normal and osteogenic media	1017:1048	Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media.					
34254733	4	74	theme	methacrylated	731:743	arg1	MeGel					754:758	MeGel	754:758	MeGel	754:758	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	74	theme	methacrylated	731:743	arg1	gelatin					745:751	methacrylated gelatin	731:751	methacrylated gelatin (MeGel)	731:759	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	75	theme	TEMPO-modified	662:675	arg1	mNCC					704:707	mNCC	704:707	mNCC	704:707	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34254733	4	75	theme	TEMPO-modified	662:675	arg1	cellulose					693:701	TEMPO-modified nanocrystalline cellulose	662:701	TEMPO-modified nanocrystalline cellulose (mNCC)	662:708	In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel).					
34812971	2	0	theme	24-well	521:527	arg1	plates					541:546	24-well polystyrene plates	521:546	24-well polystyrene plates	521:546	Biofilms were formed in 24-well polystyrene plates at 37 °C for 48 h under microaerophilia.					
34812971	3	1	theme	incubation	922:931	arg1	time					933:936	30 min incubation time	915:936	30 min incubation time	915:936	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	1	2	theme	phthalocyanine	184:197	arg1	AlClPc					209:214	AlClPc	209:214	AlClPc	209:214	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	1	2	theme	phthalocyanine	184:197	arg1	chloride					199:206	the aluminum phthalocyanine chloride	171:206	the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN)	171:259	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	9	3	theme	log10	1758:1762	arg1	casei					1746:1750	L. casei	1743:1750	L. casei (4.91 log10 CFU/mL)	1743:1770	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	3	theme	log10	1758:1762	arg1	CFU/mL					1764:1769	4.91 log10 CFU/mL	1753:1769	4.91 log10 CFU/mL	1753:1769	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	3	4	dep	gluconate	677:685	arg1	saline					713:718	phosphate-buffered saline	694:718	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	4	dep	gluconate	677:685	arg1	control					803:809	the dark toxicity control	785:809	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	4	dep	gluconate	677:685	arg1	PBS					721:723	PBS	721:723	PBS	721:723	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	9	5	theme	significant	1670:1680	arg1	reduction					1682:1690	a significant reduction	1668:1690	a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL)	1668:1807	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	11	6	theme	albicans	2064:2071	arg1	biofilm					2025:2031	the biofilm	2021:2031	the biofilm of S. mutans, L. casei, and C. albicans	2021:2071	The aPDT mediated by the nanoconjugate AlClPc + CN + L was efficient against the biofilm of S. mutans, L. casei, and C. albicans.					
34812971	1	7	theme	lactate	461:467	arg1	production					469:478	lactate production	461:478	lactate production after treatment	461:494	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	3	8	dep	evaluated	615:623	arg1	n = 9					626:630	n = 9	626:630	n = 9	626:630	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	8	9	theme	AlClPc	1591:1596	arg1	groups					1598:1603	the CN and AlClPc groups	1580:1603	groups	1598:1603	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	3	10	theme	%	661:661	arg1	CHX					688:690	CHX	688:690	CHX	688:690	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	10	theme	%	661:661	arg1	gluconate					677:685	0.12% chlorhexidine gluconate	657:685	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	10	theme	%	661:661	arg1	control					648:654	a positive control	637:654	a positive control	637:654	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	6	11	theme	antimicrobial	1294:1306	arg1	activity					1308:1315	The antimicrobial activity	1290:1315	The antimicrobial activity	1290:1315	The antimicrobial activity was analyzed by quantifying colony forming units per mL (CFU/mL) of each microorganism.					
34812971	3	12	theme	phosphate-buffered	694:711	arg1	saline					713:718	phosphate-buffered saline	694:718	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	12	theme	phosphate-buffered	694:711	arg1	PBS					721:723	PBS	721:723	PBS	721:723	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	5	13	dep	biofilm	1202:1208	arg1	composition					1210:1220	composition	1210:1220	composition	1210:1220	The biofilm composition and morphology were observed by scanning electron microscopy (SEM).					
34812971	5	13	dep	biofilm	1202:1208	arg1	morphology					1226:1235	morphology	1226:1235	morphology	1226:1235	The biofilm composition and morphology were observed by scanning electron microscopy (SEM).					
34812971	9	14	dep	showed	1661:1666	arg1	compared					1809:1816	compared	1809:1816	showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05)	1661:1855	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	1	15	theme	antimicrobial	277:289	arg1	aPDT					313:316	aPDT	313:316	aPDT	313:316	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	1	15	theme	antimicrobial	277:289	arg1	therapy					304:310	antimicrobial photodynamic therapy	277:310	antimicrobial photodynamic therapy (aPDT)	277:317	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	0	16	theme	chitosan	114:121	arg1	nanoparticles					123:135	chitosan nanoparticles	114:135	chitosan nanoparticles	114:135	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride encapsulated in chitosan nanoparticles.					
34812971	3	17	dep	used	831:834	arg1	AlClPc + CN					848:858	AlClPc + CN	848:858	AlClPc + CN	848:858	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	17	dep	used	831:834	arg1	formulations					818:829	the formulations	814:829	the formulations used (AlClPc and AlClPc + CN)	814:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	17	dep	used	831:834	arg1	AlClPc					837:842	AlClPc	837:842	AlClPc	837:842	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	9	18	theme	log10	1795:1799	arg1	albicans					1780:1787	C. albicans	1777:1787	C. albicans (2.09 log10 CFU/mL)	1777:1807	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	18	theme	log10	1795:1799	arg1	CFU/mL					1801:1806	2.09 log10 CFU/mL	1790:1806	2.09 log10 CFU/mL	1790:1806	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	5	19	theme	electron	1263:1270	arg1	microscopy					1272:1281	scanning electron microscopy	1254:1281	scanning electron microscopy (SEM)	1254:1287	The biofilm composition and morphology were observed by scanning electron microscopy (SEM).					
34812971	5	19	theme	electron	1263:1270	arg1	SEM					1284:1286	SEM	1284:1286	SEM	1284:1286	The biofilm composition and morphology were observed by scanning electron microscopy (SEM).					
34812971	1	20	theme	photodynamic	291:302	arg1	aPDT					313:316	aPDT	313:316	aPDT	313:316	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	1	20	theme	photodynamic	291:302	arg1	therapy					304:310	antimicrobial photodynamic therapy	277:310	antimicrobial photodynamic therapy (aPDT)	277:317	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	3	21	theme	energy	1124:1129	arg1	density					1131:1137	the energy density	1120:1137	the energy density of 100 J/cm2	1120:1150	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	6	22	theme	forming	1352:1358	arg1	units					1360:1364	colony forming units	1345:1364	colony forming units per mL (CFU/mL) of each microorganism	1345:1402	The antimicrobial activity was analyzed by quantifying colony forming units per mL (CFU/mL) of each microorganism.					
34812971	3	23	theme	dark	789:792	arg1	control					803:809	the dark toxicity control	785:809	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	24	theme	toxicity	794:801	arg1	control					803:809	the dark toxicity control	785:809	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	8	25	theme	aPDT	1532:1535	arg1	AlClPc + CN + L					1537:1551	the aPDT AlClPc + CN + L and CHX groups	1528:1566	AlClPc + CN + L	1537:1551	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	8	26	theme	lactate	1497:1503	arg1	dosage					1505:1510	A lower lactate dosage	1489:1510	A lower lactate dosage	1489:1510	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	9	27	theme	negative	1825:1832	arg1	p < 0.05					1847:1854	p < 0.05	1847:1854	p < 0.05	1847:1854	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	27	theme	negative	1825:1832	arg1	PBS					1842:1844	the negative control PBS	1821:1844	the negative control PBS (p < 0.05)	1821:1855	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	3	28	theme	5.8 × 10-3 mg/mL	988:1003	arg1	concentration					971:983	a final concentration	963:983	a final concentration of 5.8 × 10-3 mg/mL	963:1003	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	9	29	theme	albicans	1780:1787	arg1	viability					1699:1707	the viability	1695:1707	the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL)	1695:1807	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	6	30	theme	colony	1345:1350	arg1	units					1360:1364	colony forming units	1345:1364	colony forming units per mL (CFU/mL) of each microorganism	1345:1402	The antimicrobial activity was analyzed by quantifying colony forming units per mL (CFU/mL) of each microorganism.					
34812971	3	31	theme	visible	1039:1045	arg1	light					1047:1051	visible light	1039:1051	visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2	1039:1150	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	32	theme	final	965:969	arg1	concentration					971:983	a final concentration	963:983	a final concentration of 5.8 × 10-3 mg/mL	963:1003	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	33	theme	light	892:896	arg1	absence					881:887	the absence	877:887	the absence of light	877:896	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	7	34	theme	HSD	1467:1469	arg1	α = 0.05					1478:1485	α = 0.05	1478:1485	α = 0.05	1478:1485	Bacterial load between groups was analyzed by ANOVA and Tukey HSD tests (α = 0.05).					
34812971	7	34	theme	HSD	1467:1469	arg1	tests					1471:1475	Tukey HSD tests	1461:1475	Tukey HSD tests (α = 0.05)	1461:1486	Bacterial load between groups was analyzed by ANOVA and Tukey HSD tests (α = 0.05).					
34812971	0	35	theme	cariogenic	34:43	arg1	biofilm					45:51	multispecies cariogenic biofilm	21:51	multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride	21:96	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride encapsulated in chitosan nanoparticles.					
34812971	4	36	theme	in	1156:1157	arg1	kit					1165:1167	An in vitro kit	1153:1167	An in vitro kit	1153:1167	An in vitro kit was used to measure lactate.					
34812971	3	37	theme	following	593:601	arg1	groups					603:608	The following groups	589:608	The following groups	589:608	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	11	38	theme	nanoconjugate	1969:1981	arg1	L					1997:1997	the nanoconjugate AlClPc + CN + L	1965:1997	the nanoconjugate AlClPc + CN + L	1965:1997	The aPDT mediated by the nanoconjugate AlClPc + CN + L was efficient against the biofilm of S. mutans, L. casei, and C. albicans.					
34812971	0	39	theme	multispecies	21:32	arg1	biofilm					45:51	multispecies cariogenic biofilm	21:51	multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride	21:96	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride encapsulated in chitosan nanoparticles.					
34812971	3	40	theme	negative	731:738	arg1	CN					754:755	a negative control; 2.5% CN	729:755	a negative control; 2.5% CN as release vehicle control	729:782	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	1	41	theme	Candida	402:408	arg1	albicans					410:417	Candida albicans	402:417	Candida albicans	402:417	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	3	42	theme	100 J/cm2	1142:1150	arg1	density					1131:1137	the energy density	1120:1137	the energy density of 100 J/cm2	1120:1150	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	1	43	theme	chitosan	233:240	arg1	CN					257:258	CN	257:258	CN	257:258	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	1	43	theme	chitosan	233:240	arg1	nanoparticles					242:254	chitosan nanoparticles	233:254	chitosan nanoparticles (CN)	233:259	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	9	44	theme	log10	1728:1732	arg1	mutans					1715:1720	S. mutans	1712:1720	S. mutans (3.18 log10 CFU/mL)	1712:1740	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	44	theme	log10	1728:1732	arg1	CFU/mL					1734:1739	3.18 log10 CFU/mL	1723:1739	3.18 log10 CFU/mL	1723:1739	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	3	45	theme	control	740:746	arg1	CN					754:755	a negative control; 2.5% CN	729:755	a negative control; 2.5% CN as release vehicle control	729:782	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	7	46	theme	Tukey	1461:1465	arg1	α = 0.05					1478:1485	α = 0.05	1478:1485	α = 0.05	1478:1485	Bacterial load between groups was analyzed by ANOVA and Tukey HSD tests (α = 0.05).					
34812971	7	46	theme	Tukey	1461:1465	arg1	tests					1471:1475	Tukey HSD tests	1461:1475	Tukey HSD tests (α = 0.05)	1461:1486	Bacterial load between groups was analyzed by ANOVA and Tukey HSD tests (α = 0.05).					
34812971	3	47	theme	chlorhexidine	663:675	arg1	CHX					688:690	CHX	688:690	CHX	688:690	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	47	theme	chlorhexidine	663:675	arg1	gluconate					677:685	0.12% chlorhexidine gluconate	657:685	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	47	theme	chlorhexidine	663:675	arg1	control					648:654	a positive control	637:654	a positive control	637:654	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	5	48	theme	scanning	1254:1261	arg1	microscopy					1272:1281	scanning electron microscopy	1254:1281	scanning electron microscopy (SEM)	1254:1287	The biofilm composition and morphology were observed by scanning electron microscopy (SEM).					
34812971	5	48	theme	scanning	1254:1261	arg1	SEM					1284:1286	SEM	1284:1286	SEM	1284:1286	The biofilm composition and morphology were observed by scanning electron microscopy (SEM).					
34812971	3	49	with	device	1065:1070	arg1	660 nm					1110:1115	660 nm	1110:1115	660 nm	1110:1115	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	0	50	theme	biofilm	45:51	arg1	Photoinactivation					0:16	Photoinactivation	0:16	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride	0:96	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride encapsulated in chitosan nanoparticles.					
34812971	8	51	located	observed	1516:1523	arg2	dosage					1505:1510	A lower lactate dosage	1489:1510	A lower lactate dosage	1489:1510	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	8	51	located	observed	1516:1523	arg1	AlClPc + CN + L					1537:1551	the aPDT AlClPc + CN + L and CHX groups	1528:1566	AlClPc + CN + L	1537:1551	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	8	51	located	observed	1516:1523	arg1	groups					1561:1566	the aPDT AlClPc + CN + L and CHX groups	1528:1566	groups	1561:1566	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	3	52	theme	LED	1061:1063	arg1	device					1065:1070	a LED device	1059:1070	a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm	1059:1115	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	52	theme	LED	1061:1063	arg1	AlClPc + CN + L					1088:1102	AlClPc + CN + L	1088:1102	AlClPc + CN + L	1088:1102	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	52	theme	LED	1061:1063	arg1	AlClPc + L					1073:1082	AlClPc + L	1073:1082	AlClPc + L	1073:1082	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	9	53	from	reduction	1682:1690	arg1	viability					1699:1707	the viability	1695:1707	the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL)	1695:1807	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	54	theme	mutans	1715:1720	arg1	viability					1699:1707	the viability	1695:1707	the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL)	1695:1807	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	10	55	theme	isolated	1869:1876	arg1	AlClPc					1878:1883	isolated AlClPc	1869:1883	isolated AlClPc	1869:1883	aPDT using isolated AlClPc was similar to PBS to the three microorganisms (p > 0.05).					
34812971	11	56	theme	casei	2050:2054	arg1	biofilm					2025:2031	the biofilm	2021:2031	the biofilm of S. mutans, L. casei, and C. albicans	2021:2071	The aPDT mediated by the nanoconjugate AlClPc + CN + L was efficient against the biofilm of S. mutans, L. casei, and C. albicans.					
34812971	0	57	theme	aluminum	65:72	arg1	chloride					89:96	aluminum phthalocyanine chloride	65:96	aluminum phthalocyanine chloride	65:96	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride encapsulated in chitosan nanoparticles.					
34812971	1	58	theme	multispecies	322:333	arg1	biofilm					335:341	multispecies biofilm	322:341	multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment	322:494	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	0	59	theme	phthalocyanine	74:87	arg1	chloride					89:96	aluminum phthalocyanine chloride	65:96	aluminum phthalocyanine chloride	65:96	Photoinactivation of multispecies cariogenic biofilm mediated by aluminum phthalocyanine chloride encapsulated in chitosan nanoparticles.					
34812971	4	60	used	used	1173:1176	arg2	kit					1165:1167	An in vitro kit	1153:1167	An in vitro kit	1153:1167	An in vitro kit was used to measure lactate.					
34812971	7	61	theme	Bacterial	1405:1413	arg1	load					1415:1418	Bacterial load	1405:1418	Bacterial load between groups	1405:1433	Bacterial load between groups was analyzed by ANOVA and Tukey HSD tests (α = 0.05).					
34812971	3	62	theme	%	752:752	arg1	CN					754:755	a negative control; 2.5% CN	729:755	a negative control; 2.5% CN as release vehicle control	729:782	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	63	from	concentration	971:983	arg1	photosensitizers					943:958	the photosensitizers	939:958	the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL	939:1003	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	8	64	theme	lower	1491:1495	arg1	dosage					1505:1510	A lower lactate dosage	1489:1510	A lower lactate dosage	1489:1510	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	8	65	theme	CHX	1557:1559	arg1	groups					1561:1566	the aPDT AlClPc + CN + L and CHX groups	1528:1566	groups	1561:1566	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	1	66	theme	antimicrobial	434:446	arg1	activity					448:455	the antimicrobial activity	430:455	the antimicrobial activity	430:455	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	3	67	theme	positive	639:646	arg1	gluconate					677:685	0.12% chlorhexidine gluconate	657:685	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	67	theme	positive	639:646	arg1	control					648:654	a positive control	637:654	a positive control	637:654	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	4	68	dep	in	1156:1157	arg1	vitro					1159:1163	vitro	1159:1163	vitro	1159:1163	An in vitro kit was used to measure lactate.					
34812971	9	69	theme	nanoconjugate	1631:1643	arg1	AlClPc + CN + L					1645:1659	the nanoconjugate AlClPc + CN + L	1627:1659	the nanoconjugate AlClPc + CN + L	1627:1659	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	70	theme	control	1834:1840	arg1	p < 0.05					1847:1854	p < 0.05	1847:1854	p < 0.05	1847:1854	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	70	theme	control	1834:1840	arg1	PBS					1842:1844	the negative control PBS	1821:1844	the negative control PBS (p < 0.05)	1821:1855	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	9	71	theme	casei	1746:1750	arg1	viability					1699:1707	the viability	1695:1707	the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL)	1695:1807	The aPDT mediated by the nanoconjugate AlClPc + CN + L showed a significant reduction in the viability of S. mutans (3.18 log10 CFU/mL), L. casei (4.91 log10 CFU/mL), and C. albicans (2.09 log10 CFU/mL) compared to the negative control PBS (p < 0.05).					
34812971	11	72	theme	mutans	2039:2044	arg1	biofilm					2025:2031	the biofilm	2021:2031	the biofilm of S. mutans, L. casei, and C. albicans	2021:2071	The aPDT mediated by the nanoconjugate AlClPc + CN + L was efficient against the biofilm of S. mutans, L. casei, and C. albicans.					
34812971	8	73	theme	CN	1584:1585	arg1	groups					1598:1603	the CN and AlClPc groups	1580:1603	groups	1598:1603	A lower lactate dosage was observed in the aPDT AlClPc + CN + L and CHX groups compared to the CN and AlClPc groups.					
34812971	3	74	theme	formulations	818:829	arg1	control					803:809	the dark toxicity control	785:809	0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN)	657:859	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	3	75	theme	vehicle	768:774	arg1	control					776:782	release vehicle control	760:782	release vehicle control	760:782	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	2	76	theme	polystyrene	529:539	arg1	plates					541:546	24-well polystyrene plates	521:546	24-well polystyrene plates	521:546	Biofilms were formed in 24-well polystyrene plates at 37 °C for 48 h under microaerophilia.					
34812971	11	77	theme	AlClPc + CN +	1983:1995	arg1	L					1997:1997	the nanoconjugate AlClPc + CN + L	1965:1997	the nanoconjugate AlClPc + CN + L	1965:1997	The aPDT mediated by the nanoconjugate AlClPc + CN + L was efficient against the biofilm of S. mutans, L. casei, and C. albicans.					
34812971	3	78	theme	30 min	915:920	arg1	time					933:936	30 min incubation time	915:936	30 min incubation time	915:936	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
34812971	1	79	theme	aluminum	175:182	arg1	AlClPc					209:214	AlClPc	209:214	AlClPc	209:214	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	1	79	theme	aluminum	175:182	arg1	chloride					199:206	the aluminum phthalocyanine chloride	171:206	the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN)	171:259	This study aimed to characterize the aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm composed of Streptococcus mutans, Lactobacillus casei, and Candida albicans to analyze the antimicrobial activity and lactate production after treatment.					
34812971	6	80	theme	microorganism	1390:1402	arg1	mL					1370:1371	mL	1370:1371	mL (CFU/mL) of each microorganism	1370:1402	The antimicrobial activity was analyzed by quantifying colony forming units per mL (CFU/mL) of each microorganism.					
34812971	6	80	theme	microorganism	1390:1402	arg1	CFU/mL					1374:1379	CFU/mL	1374:1379	CFU/mL	1374:1379	The antimicrobial activity was analyzed by quantifying colony forming units per mL (CFU/mL) of each microorganism.					
34812971	3	81	theme	release	760:766	arg1	control					776:782	release vehicle control	760:782	release vehicle control	760:782	The following groups were evaluated (n = 9): as a positive control, 0.12% chlorhexidine gluconate (CHX); phosphate-buffered saline (PBS) as a negative control; 2.5% CN as release vehicle control; the dark toxicity control of the formulations used (AlClPc and AlClPc + CN) was verified in the absence of light; for aPDT, after 30 min incubation time, the photosensitizers at a final concentration of 5.8 × 10-3 mg/mL were photoirradiated for 1 min by visible light using a LED device (AlClPc + L and AlClPc + CN + L) with 660 nm at the energy density of 100 J/cm2.					
31987952	0	0	theme	turmeric	83:90	arg1	oil					92:94	zedoary turmeric oil	75:94	zedoary turmeric oil	75:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	0	1	theme	zedoary	75:81	arg1	oil					92:94	zedoary turmeric oil	75:94	zedoary turmeric oil	75:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	9	2	theme	CS-ZTO-SLN	1145:1154	arg1	higher					1160:1165	higher	1160:1165	higher	1160:1165	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	9	2	theme	CS-ZTO-SLN	1145:1154	arg1	accumulation					1129:1140	The liver accumulation	1119:1140	The liver accumulation of CS-ZTO-SLN	1119:1154	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	9	3	theme	ZTO	1282:1284	arg1	bioavailability					1263:1277	the bioavailability	1259:1277	the bioavailability of ZTO	1259:1284	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	6	4	dep	uptake	857:862	arg1	The					853:855	The	853:855	The	853:855	The uptake and distribution of drug were evaluated in vitro and in vivo.					
31987952	5	5	theme	lipid	702:706	arg1	SLN					723:725	SLN	723:725	SLN	723:725	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	5	theme	lipid	702:706	arg1	nanoparticles					708:720	Chitosan (CS)-coated solid lipid nanoparticles	675:720	Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO	675:742	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	3	6	theme	hydrophobic	382:392	arg1	drug					394:397	dissolve hydrophobic drug	373:397	dissolve hydrophobic drug	373:397	Solid lipid nanoparticles can provide hydrophobic environment to dissolve hydrophobic drug and solidify the oily active composition to decrease the volatility and facilitate the medication.					
31987952	0	7	theme	oil	92:94	arg1	delivery					63:70	enhanced liver delivery	48:70	enhanced liver delivery of zedoary turmeric oil	48:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	10	8	theme	formulation	1390:1400	arg1	features					1374:1381	the features	1370:1381	the features of ZTO formulation	1370:1400	The results suggested that SLN coated with CS improved the features of ZTO formulation and efficiently deliver drug to the liver.					
31987952	5	9	theme	transmission	813:824	arg1	TEM					847:849	TEM	847:849	TEM	847:849	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	9	theme	transmission	813:824	arg1	microscope					835:844	transmission electron microscope	813:844	transmission electron microscope (TEM)	813:850	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	3	10	theme	lipid	314:318	arg1	nanoparticles					320:332	Solid lipid nanoparticles	308:332	Solid lipid nanoparticles	308:332	Solid lipid nanoparticles can provide hydrophobic environment to dissolve hydrophobic drug and solidify the oily active composition to decrease the volatility and facilitate the medication.					
31987952	1	11	theme	Zedoary	105:111	arg1	oil					122:124	Zedoary turmeric oil	105:124	Zedoary turmeric oil (ZTO)	105:130	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	1	11	theme	Zedoary	105:111	arg1	ZTO					127:129	ZTO	127:129	ZTO	127:129	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	2	12	theme	medication	243:252	arg1	difficulty					229:238	difficulty	229:238	difficulty of medication	229:252	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	2	12	theme	medication	243:252	arg1	insolubility					190:201	insolubility	190:201	insolubility	190:201	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	2	12	theme	medication	243:252	arg1	bioavailability					208:222	low bioavailability	204:222	low bioavailability	204:222	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	2	12	theme	medication	243:252	arg1	volatility					178:187	its volatility	174:187	its volatility	174:187	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	1	13	theme	strong	138:143	arg1	activity					155:162	a strong antitumor activity	136:162	a strong antitumor activity	136:162	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	10	14	theme	ZTO	1386:1388	arg1	formulation					1390:1400	ZTO formulation	1386:1400	ZTO formulation	1386:1400	The results suggested that SLN coated with CS improved the features of ZTO formulation and efficiently deliver drug to the liver.					
31987952	5	15	theme	dynamic	781:787	arg1	DLS					804:806	DLS	804:806	DLS	804:806	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	15	theme	dynamic	781:787	arg1	scanner					795:801	dynamic laser scanner	781:801	dynamic laser scanner (DLS)	781:807	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	8	16	theme	surface	1050:1056	arg1	charge					1058:1063	the surface charge	1046:1063	the surface charge of particles from -8.93 ± 1.92 mV	1046:1097	CS coating inverted the surface charge of particles from -8.93 ± 1.92 mV to +9.12 ± 2.03 mV.					
31987952	1	17	theme	antitumor	145:153	arg1	activity					155:162	a strong antitumor activity	136:162	a strong antitumor activity	136:162	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	8	18	theme	CS	1026:1027	arg1	coating					1029:1035	CS coating	1026:1035	CS coating	1026:1035	CS coating inverted the surface charge of particles from -8.93 ± 1.92 mV to +9.12 ± 2.03 mV.					
31987952	1	19	contain	has	132:134	arg1	oil					122:124	Zedoary turmeric oil	105:124	Zedoary turmeric oil (ZTO)	105:130	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	1	19	contain	has	132:134	arg1	ZTO					127:129	ZTO	127:129	ZTO	127:129	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	1	19	contain	has	132:134	arg2	activity					155:162	a strong antitumor activity	136:162	a strong antitumor activity	136:162	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	3	20	theme	dissolve	373:380	arg1	drug					394:397	dissolve hydrophobic drug	373:397	dissolve hydrophobic drug	373:397	Solid lipid nanoparticles can provide hydrophobic environment to dissolve hydrophobic drug and solidify the oily active composition to decrease the volatility and facilitate the medication.					
31987952	4	21	theme	recent	548:553	arg1	years					555:559	recent years	548:559	recent years	548:559	Chitosan has been widely used in pharmaceutics in recent years and coating with chitosan further enhances the internalization of particles by cells due to charge attract.					
31987952	4	22	used	used	523:526	arg2	Chitosan					498:505	Chitosan	498:505	Chitosan	498:505	Chitosan has been widely used in pharmaceutics in recent years and coating with chitosan further enhances the internalization of particles by cells due to charge attract.					
31987952	9	23	theme	chitosan-uncoated	1181:1197	arg1	ZTO-SLN					1172:1178	ZTO-SLN	1172:1178	ZTO-SLN (chitosan-uncoated particles)	1172:1208	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	9	23	theme	chitosan-uncoated	1181:1197	arg1	particles					1199:1207	chitosan-uncoated particles	1181:1207	chitosan-uncoated particles	1181:1207	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	3	24	theme	hydrophobic	346:356	arg1	environment					358:368	hydrophobic environment	346:368	hydrophobic environment to dissolve hydrophobic drug	346:397	Solid lipid nanoparticles can provide hydrophobic environment to dissolve hydrophobic drug and solidify the oily active composition to decrease the volatility and facilitate the medication.					
31987952	0	25	theme	solid	18:22	arg1	nanoparticles					30:42	solid lipid nanoparticles	18:42	solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil	18:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	4	26	theme	particles	627:635	arg1	internalization					608:622	the internalization	604:622	the internalization of particles	604:635	Chitosan has been widely used in pharmaceutics in recent years and coating with chitosan further enhances the internalization of particles by cells due to charge attract.					
31987952	4	27	from	pharmaceutics	531:543	arg1	years					555:559	recent years	548:559	recent years	548:559	Chitosan has been widely used in pharmaceutics in recent years and coating with chitosan further enhances the internalization of particles by cells due to charge attract.					
31987952	5	28	theme	-coated	688:694	arg1	SLN					723:725	SLN	723:725	SLN	723:725	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	28	theme	-coated	688:694	arg1	nanoparticles					708:720	Chitosan (CS)-coated solid lipid nanoparticles	675:720	Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO	675:742	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	2	29	theme	low	204:206	arg1	bioavailability					208:222	low bioavailability	204:222	low bioavailability	204:222	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	3	30	theme	active	421:426	arg1	composition					428:438	the oily active composition to decrease the volatility and facilitate the medication	412:495	the oily active composition to decrease the volatility and facilitate the medication	412:495	Solid lipid nanoparticles can provide hydrophobic environment to dissolve hydrophobic drug and solidify the oily active composition to decrease the volatility and facilitate the medication.					
31987952	5	31	theme	solid	696:700	arg1	SLN					723:725	SLN	723:725	SLN	723:725	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	31	theme	solid	696:700	arg1	nanoparticles					708:720	Chitosan (CS)-coated solid lipid nanoparticles	675:720	Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO	675:742	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	0	32	theme	lipid	24:28	arg1	nanoparticles					30:42	solid lipid nanoparticles	18:42	solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil	18:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	5	33	theme	laser	789:793	arg1	DLS					804:806	DLS	804:806	DLS	804:806	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	33	theme	laser	789:793	arg1	scanner					795:801	dynamic laser scanner	781:801	dynamic laser scanner (DLS)	781:807	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	0	34	theme	liver	57:61	arg1	delivery					63:70	enhanced liver delivery	48:70	enhanced liver delivery of zedoary turmeric oil	48:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	8	35	from	-8.93 ± 1.92 mV	1083:1097	arg1	charge					1058:1063	the surface charge	1046:1063	the surface charge of particles from -8.93 ± 1.92 mV	1046:1097	CS coating inverted the surface charge of particles from -8.93 ± 1.92 mV to +9.12 ± 2.03 mV.					
31987952	7	36	theme	CS-ZTO-SLN	959:968	arg1	sizes					938:942	The average sizes	926:942	The average sizes of ZTO-SLN and CS-ZTO-SLN	926:968	The average sizes of ZTO-SLN and CS-ZTO-SLN were 134.3 ± 3.42 nm and 210.7 ± 4.59 nm, respectively.					
31987952	7	36	theme	CS-ZTO-SLN	959:968	arg1	134.3 ± 3.42 nm					975:989	134.3 ± 3.42 nm	975:989	134.3 ± 3.42 nm	975:989	The average sizes of ZTO-SLN and CS-ZTO-SLN were 134.3 ± 3.42 nm and 210.7 ± 4.59 nm, respectively.					
31987952	2	37	dep	liquid	268:273	arg1	limit					275:279	limit	275:279	limit its clinical applications	275:305	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	0	38	theme	enhanced	48:55	arg1	delivery					63:70	enhanced liver delivery	48:70	enhanced liver delivery of zedoary turmeric oil	48:94	Chitosan mediated solid lipid nanoparticles for enhanced liver delivery of zedoary turmeric oil in vivo.					
31987952	9	39	theme	tissue	1225:1230	arg1	homogenate					1232:1241	tissue homogenate	1225:1241	tissue homogenate using HPLC	1225:1252	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	1	40	theme	turmeric	113:120	arg1	oil					122:124	Zedoary turmeric oil	105:124	Zedoary turmeric oil (ZTO)	105:130	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	1	40	theme	turmeric	113:120	arg1	ZTO					127:129	ZTO	127:129	ZTO	127:129	Zedoary turmeric oil (ZTO) has a strong antitumor activity.					
31987952	6	41	theme	drug	884:887	arg1	distribution					868:879	distribution	868:879	distribution	868:879	The uptake and distribution of drug were evaluated in vitro and in vivo.					
31987952	6	41	theme	drug	884:887	arg1	uptake					857:862	uptake	857:862	uptake	857:862	The uptake and distribution of drug were evaluated in vitro and in vivo.					
31987952	5	42	theme	Chitosan	675:682	arg1	SLN					723:725	SLN	723:725	SLN	723:725	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	42	theme	Chitosan	675:682	arg1	nanoparticles					708:720	Chitosan (CS)-coated solid lipid nanoparticles	675:720	Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO	675:742	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	9	43	theme	homogenate	1232:1241	arg1	analysis					1213:1220	analysis	1213:1220	analysis of tissue homogenate using HPLC	1213:1252	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	7	44	theme	ZTO-SLN	947:953	arg1	sizes					938:942	The average sizes	926:942	The average sizes of ZTO-SLN and CS-ZTO-SLN	926:968	The average sizes of ZTO-SLN and CS-ZTO-SLN were 134.3 ± 3.42 nm and 210.7 ± 4.59 nm, respectively.					
31987952	7	44	theme	ZTO-SLN	947:953	arg1	134.3 ± 3.42 nm					975:989	134.3 ± 3.42 nm	975:989	134.3 ± 3.42 nm	975:989	The average sizes of ZTO-SLN and CS-ZTO-SLN were 134.3 ± 3.42 nm and 210.7 ± 4.59 nm, respectively.					
31987952	8	45	theme	particles	1068:1076	arg1	charge					1058:1063	the surface charge	1046:1063	the surface charge of particles from -8.93 ± 1.92 mV	1046:1097	CS coating inverted the surface charge of particles from -8.93 ± 1.92 mV to +9.12 ± 2.03 mV.					
31987952	7	46	theme	average	930:936	arg1	sizes					938:942	The average sizes	926:942	The average sizes of ZTO-SLN and CS-ZTO-SLN	926:968	The average sizes of ZTO-SLN and CS-ZTO-SLN were 134.3 ± 3.42 nm and 210.7 ± 4.59 nm, respectively.					
31987952	7	46	theme	average	930:936	arg1	134.3 ± 3.42 nm					975:989	134.3 ± 3.42 nm	975:989	134.3 ± 3.42 nm	975:989	The average sizes of ZTO-SLN and CS-ZTO-SLN were 134.3 ± 3.42 nm and 210.7 ± 4.59 nm, respectively.					
31987952	2	47	theme	clinical	285:292	arg1	applications					294:305	its clinical applications	281:305	its clinical applications	281:305	However, its volatility, insolubility, low bioavailability, and difficulty of medication owing to oily liquid limit its clinical applications.					
31987952	4	48	dep	charge	653:658	arg1	attract					660:666	attract	660:666	attract	660:666	Chitosan has been widely used in pharmaceutics in recent years and coating with chitosan further enhances the internalization of particles by cells due to charge attract.					
31987952	9	49	theme	liver	1123:1127	arg1	higher					1160:1165	higher	1160:1165	higher	1160:1165	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	9	49	theme	liver	1123:1127	arg1	accumulation					1129:1140	The liver accumulation	1119:1140	The liver accumulation of CS-ZTO-SLN	1119:1154	The liver accumulation of CS-ZTO-SLN was higher than ZTO-SLN (chitosan-uncoated particles) by analysis of tissue homogenate using HPLC, and the bioavailability of ZTO was also obviously improved.					
31987952	5	50	theme	electron	826:833	arg1	TEM					847:849	TEM	847:849	TEM	847:849	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	5	50	theme	electron	826:833	arg1	microscope					835:844	transmission electron microscope	813:844	transmission electron microscope (TEM)	813:850	Here, Chitosan (CS)-coated solid lipid nanoparticles (SLN) loaded with ZTO was prepared and characterized using dynamic laser scanner (DLS) and transmission electron microscope (TEM).					
31987952	3	51	theme	Solid	308:312	arg1	nanoparticles					320:332	Solid lipid nanoparticles	308:332	Solid lipid nanoparticles	308:332	Solid lipid nanoparticles can provide hydrophobic environment to dissolve hydrophobic drug and solidify the oily active composition to decrease the volatility and facilitate the medication.					
31987952	4	52	with	coating	565:571	arg1	chitosan					578:585	chitosan	578:585	chitosan	578:585	Chitosan has been widely used in pharmaceutics in recent years and coating with chitosan further enhances the internalization of particles by cells due to charge attract.					
33607143	4	0	theme	variance	809:816	arg1	analysis					797:804	analysis	797:804	analysis of variance (ANOVA)	797:824	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	2	1	theme	biopharmaceutical	340:356	arg1	system					373:378	the biopharmaceutical classification system	336:378	the biopharmaceutical classification system	336:378	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	7	2	theme	delivery	1284:1291	arg1	system					1293:1298	this chitosan-based nano-drug delivery system	1254:1298	this chitosan-based nano-drug delivery system	1254:1298	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	2	theme	delivery	1284:1291	arg1	model					1377:1381	a drug model	1370:1381	a drug model	1370:1381	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	2	theme	delivery	1284:1291	arg1	candidate					1328:1336	an ideal candidate	1319:1336	an ideal candidate for oral delivery of the CIP	1319:1365	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	1	3	theme	oral	251:254	arg1	delivery					256:263	oral delivery	251:263	oral delivery of ciprofloxacin hydrochloride (CIP)	251:300	This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP).					
33607143	2	4	contain	has	425:427	arg2	problems					434:441	some problems	429:441	some problems	429:441	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	2	4	contain	has	425:427	arg1	it					422:423	it	422:423	it	422:423	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	1	5	theme	safe	169:172	arg1	carrier					184:190	a safe nano-drug carrier	167:190	a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP)	167:300	This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP).					
33607143	4	6	theme	optimal	742:748	arg1	efficiency					763:772	the optimal drug loading efficiency	738:772	the optimal drug loading efficiency (57%)	738:778	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	4	6	theme	optimal	742:748	arg1	%					777:777	57%	775:777	57%	775:777	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	5	7	theme	various	899:905	arg1	saline					943:948	phosphate buffer saline	926:948	phosphate buffer saline (pH = 7.4)	926:959	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	7	theme	various	899:905	arg1	conditions					907:916	two various conditions	895:916	two various conditions	895:916	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	7	theme	various	899:905	arg1	HCl					971:973	0.1 N HCl	965:973	0.1 N HCl	965:973	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	3	8	theme	CNCbl	539:543	arg1	conjugate					466:474	Novel conjugate	460:474	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl	460:543	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	5	9	theme	release	875:881	arg1	patterns					883:890	controlled release patterns	864:890	controlled release patterns	864:890	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	7	10	theme	drug	1372:1375	arg1	system					1293:1298	this chitosan-based nano-drug delivery system	1254:1298	this chitosan-based nano-drug delivery system	1254:1298	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	10	theme	drug	1372:1375	arg1	model					1377:1381	a drug model	1370:1381	a drug model	1370:1381	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	11	theme	CIP	1363:1365	arg1	delivery					1347:1354	oral delivery	1342:1354	oral delivery of the CIP	1342:1365	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	3	12	theme	weight	493:498	arg1	chitosan					500:507	low molecular weight chitosan	479:507	low molecular weight chitosan, as a natural biopolymer,	479:533	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	1	13	theme	ciprofloxacin	268:280	arg1	CIP					297:299	CIP	297:299	CIP	297:299	This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP).					
33607143	1	13	theme	ciprofloxacin	268:280	arg1	hydrochloride					282:294	ciprofloxacin hydrochloride	268:294	ciprofloxacin hydrochloride (CIP)	268:300	This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP).					
33607143	5	14	theme	buffer	936:941	arg1	saline					943:948	phosphate buffer saline	926:948	phosphate buffer saline (pH = 7.4)	926:959	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	14	theme	buffer	936:941	arg1	conditions					907:916	two various conditions	895:916	two various conditions	895:916	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	14	theme	buffer	936:941	arg1	pH = 7.4					951:958	pH = 7.4	951:958	pH = 7.4	951:958	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	6	15	theme	Functionalized	976:989	arg1	carrier					1008:1014	Functionalized nano-drug-loaded carrier	976:1014	Functionalized nano-drug-loaded carrier	976:1014	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	4	16	theme	CIP	640:642	arg1	loading					629:635	The loading	625:635	The loading of CIP	625:642	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	4	16	theme	CIP	640:642	arg1	optimized					648:656	optimized	648:656	optimized by the Design-Expert software and the central composite design method	648:726	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	1	17	theme	nano-drug	174:182	arg1	carrier					184:190	a safe nano-drug carrier	167:190	a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP)	167:300	This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP).					
33607143	5	18	theme	drug	836:839	arg1	studies					849:855	In-vitro drug release studies	827:855	In-vitro drug release studies	827:855	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	7	19	theme	CIP-loaded	1234:1243	arg1	carrier					1245:1251	the CIP-loaded carrier	1230:1251	the CIP-loaded carrier	1230:1251	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	3	20	theme	natural	515:521	arg1	biopolymer					523:532	a natural biopolymer	513:532	a natural biopolymer	513:532	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	7	21	theme	chitosan-based	1259:1272	arg1	system					1293:1298	this chitosan-based nano-drug delivery system	1254:1298	this chitosan-based nano-drug delivery system	1254:1298	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	21	theme	chitosan-based	1259:1272	arg1	model					1377:1381	a drug model	1370:1381	a drug model	1370:1381	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	21	theme	chitosan-based	1259:1272	arg1	candidate					1328:1336	an ideal candidate	1319:1336	an ideal candidate for oral delivery of the CIP	1319:1365	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	0	22	theme	molecular	4:12	arg1	evaluation					117:126	evaluation	117:126	evaluation	117:126	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	22	theme	molecular	4:12	arg1	Preparation					101:111	Preparation	101:111	Preparation	101:111	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	22	theme	molecular	4:12	arg1	nanoparticles					45:57	Low molecular weight chitosan-cyanocobalamin nanoparticles	0:57	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.	0:127	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	7	23	theme	drug	1188:1191	arg1	carrier					1193:1199	the chitosan-based drug carrier	1169:1199	the chitosan-based drug carrier	1169:1199	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	4	24	theme	composite	704:712	arg1	method					721:726	the central composite design method	692:726	the central composite design method	692:726	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	2	25	theme	low	385:387	arg1	solubility					389:398	low solubility	385:398	low solubility	385:398	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	0	26	theme	Low	0:2	arg1	evaluation					117:126	evaluation	117:126	evaluation	117:126	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	26	theme	Low	0:2	arg1	Preparation					101:111	Preparation	101:111	Preparation	101:111	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	26	theme	Low	0:2	arg1	nanoparticles					45:57	Low molecular weight chitosan-cyanocobalamin nanoparticles	0:57	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.	0:127	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	1	27	theme	hydrochloride	282:294	arg1	delivery					256:263	oral delivery	251:263	oral delivery of ciprofloxacin hydrochloride (CIP)	251:300	This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP).					
33607143	4	28	theme	central	696:702	arg1	method					721:726	the central composite design method	692:726	the central composite design method	692:726	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	0	29	theme	chitosan-cyanocobalamin	21:43	arg1	evaluation					117:126	evaluation	117:126	evaluation	117:126	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	29	theme	chitosan-cyanocobalamin	21:43	arg1	Preparation					101:111	Preparation	101:111	Preparation	101:111	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	29	theme	chitosan-cyanocobalamin	21:43	arg1	nanoparticles					45:57	Low molecular weight chitosan-cyanocobalamin nanoparticles	0:57	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.	0:127	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	7	30	theme	oral	1342:1345	arg1	delivery					1347:1354	oral delivery	1342:1354	oral delivery of the CIP	1342:1365	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	5	31	theme	0.1 N	965:969	arg1	conditions					907:916	two various conditions	895:916	two various conditions	895:916	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	31	theme	0.1 N	965:969	arg1	HCl					971:973	0.1 N HCl	965:973	0.1 N HCl	965:973	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	6	32	theme	less	1080:1083	arg1	drug					1060:1063	free drug	1055:1063	free drug	1055:1063	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	6	32	theme	less	1080:1083	arg1	size					1075:1078	particle size	1066:1078	particle size less than 100 nm as well as positive zeta potential	1066:1130	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	6	33	theme	nano-drug-loaded	991:1006	arg1	carrier					1008:1014	Functionalized nano-drug-loaded carrier	976:1014	Functionalized nano-drug-loaded carrier	976:1014	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	0	34	theme	weight	14:19	arg1	evaluation					117:126	evaluation	117:126	evaluation	117:126	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	34	theme	weight	14:19	arg1	Preparation					101:111	Preparation	101:111	Preparation	101:111	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	34	theme	weight	14:19	arg1	nanoparticles					45:57	Low molecular weight chitosan-cyanocobalamin nanoparticles	0:57	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.	0:127	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	6	35	theme	positive	1108:1115	arg1	potential					1122:1130	positive zeta potential	1108:1130	100 nm as well as positive zeta potential	1090:1130	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	3	36	theme	chitosan	500:507	arg1	conjugate					466:474	Novel conjugate	460:474	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl	460:543	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	3	37	theme	Novel	460:464	arg1	conjugate					466:474	Novel conjugate	460:474	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl	460:543	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	4	38	theme	Design-Expert	665:677	arg1	software					679:686	the Design-Expert software	661:686	the Design-Expert software	661:686	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	7	39	theme	chitosan-based	1173:1186	arg1	carrier					1193:1199	the chitosan-based drug carrier	1169:1199	the chitosan-based drug carrier	1169:1199	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	5	40	theme	In-vitro	827:834	arg1	studies					849:855	In-vitro drug release studies	827:855	In-vitro drug release studies	827:855	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	7	41	theme	ideal	1322:1326	arg1	system					1293:1298	this chitosan-based nano-drug delivery system	1254:1298	this chitosan-based nano-drug delivery system	1254:1298	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	41	theme	ideal	1322:1326	arg1	candidate					1328:1336	an ideal candidate	1319:1336	an ideal candidate for oral delivery of the CIP	1319:1365	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	42	theme	carrier	1245:1251	arg1	features					1218:1225	the suitable features	1205:1225	the suitable features of the CIP-loaded carrier	1205:1251	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	42	theme	carrier	1245:1251	arg1	properties					1155:1164	the beneficial properties	1140:1164	the beneficial properties of the chitosan-based drug carrier	1140:1199	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	3	43	theme	drug	571:574	arg1	loading					576:582	drug loading	571:582	drug loading	571:582	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	2	44	with	IV	330:331	arg1	solubility					389:398	low solubility	385:398	low solubility	385:398	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	2	44	with	IV	330:331	arg1	permeabilityA					404:416	permeabilityA	404:416	permeabilityA	404:416	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	3	45	theme	low	479:481	arg1	weight					493:498	low molecular weight	479:498	low molecular weight chitosan, as a natural biopolymer,	479:533	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	0	46	theme	controlled	63:72	arg1	delivery					74:81	controlled delivery	63:81	controlled delivery of ciprofloxacin	63:98	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	7	47	theme	beneficial	1144:1153	arg1	properties					1155:1164	the beneficial properties	1140:1164	the beneficial properties of the chitosan-based drug carrier	1140:1199	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	0	48	dep	nanoparticles	45:57	arg1	evaluation					117:126	evaluation	117:126	evaluation	117:126	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	48	dep	nanoparticles	45:57	arg1	Preparation					101:111	Preparation	101:111	Preparation	101:111	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	0	48	dep	nanoparticles	45:57	arg1	nanoparticles					45:57	Low molecular weight chitosan-cyanocobalamin nanoparticles	0:57	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.	0:127	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	3	49	theme	molecular	483:491	arg1	weight					493:498	low molecular weight	479:498	low molecular weight chitosan, as a natural biopolymer,	479:533	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	6	50	theme	zeta	1117:1120	arg1	potential					1122:1130	positive zeta potential	1108:1130	100 nm as well as positive zeta potential	1090:1130	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	4	51	theme	drug	750:753	arg1	efficiency					763:772	the optimal drug loading efficiency	738:772	the optimal drug loading efficiency (57%)	738:778	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	4	51	theme	drug	750:753	arg1	%					777:777	57%	775:777	57%	775:777	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	3	52	theme	in-vitro	588:595	arg1	release					602:608	in-vitro drug release	588:608	in-vitro drug release	588:608	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	6	53	dep	much	1039:1042	arg1	as					1044:1045	as	1044:1045	as	1044:1045	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	2	54	theme	class	324:328	arg1	IV					330:331	class IV	324:331	class IV of the biopharmaceutical classification system with low solubility and permeabilityA	324:416	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	6	55	theme	free	1055:1058	arg1	drug					1060:1063	free drug	1055:1063	free drug	1055:1063	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	6	55	theme	free	1055:1058	arg1	size					1075:1078	particle size	1066:1078	particle size less than 100 nm as well as positive zeta potential	1066:1130	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	5	56	theme	release	841:847	arg1	studies					849:855	In-vitro drug release studies	827:855	In-vitro drug release studies	827:855	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	3	57	theme	drug	597:600	arg1	release					602:608	in-vitro drug release	588:608	in-vitro drug release	588:608	Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed.					
33607143	4	58	theme	design	714:719	arg1	method					721:726	the central composite design method	692:726	the central composite design method	692:726	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	5	59	theme	controlled	864:873	arg1	patterns					883:890	controlled release patterns	864:890	controlled release patterns	864:890	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	6	60	theme	particle	1066:1073	arg1	drug					1060:1063	free drug	1055:1063	free drug	1055:1063	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	6	60	theme	particle	1066:1073	arg1	size					1075:1078	particle size	1066:1078	particle size less than 100 nm as well as positive zeta potential	1066:1130	Functionalized nano-drug-loaded carrier showed cytotoxicity as much as that of free drug, particle size less than 100 nm as well as positive zeta potential.					
33607143	2	61	dep	classified	310:319	arg1	so					419:420	so	419:420	so	419:420	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	0	62	theme	ciprofloxacin	86:98	arg1	delivery					74:81	controlled delivery	63:81	controlled delivery of ciprofloxacin	63:98	Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation.					
33607143	7	63	theme	nano-drug	1274:1282	arg1	system					1293:1298	this chitosan-based nano-drug delivery system	1254:1298	this chitosan-based nano-drug delivery system	1254:1298	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	63	theme	nano-drug	1274:1282	arg1	model					1377:1381	a drug model	1370:1381	a drug model	1370:1381	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	63	theme	nano-drug	1274:1282	arg1	candidate					1328:1336	an ideal candidate	1319:1336	an ideal candidate for oral delivery of the CIP	1319:1365	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	2	64	theme	system	373:378	arg1	IV					330:331	class IV	324:331	class IV of the biopharmaceutical classification system with low solubility and permeabilityA	324:416	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	5	65	theme	phosphate	926:934	arg1	saline					943:948	phosphate buffer saline	926:948	phosphate buffer saline (pH = 7.4)	926:959	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	65	theme	phosphate	926:934	arg1	conditions					907:916	two various conditions	895:916	two various conditions	895:916	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	5	65	theme	phosphate	926:934	arg1	pH = 7.4					951:958	pH = 7.4	951:958	pH = 7.4	951:958	In-vitro drug release studies showed controlled release patterns in two various conditions, namely phosphate buffer saline (pH = 7.4) and 0.1 N HCl.					
33607143	4	66	theme	loading	755:761	arg1	efficiency					763:772	the optimal drug loading efficiency	738:772	the optimal drug loading efficiency (57%)	738:778	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	4	66	theme	loading	755:761	arg1	%					777:777	57%	775:777	57%	775:777	The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug loading efficiency (57%) was obtained via analysis of variance (ANOVA).					
33607143	7	67	theme	carrier	1193:1199	arg1	features					1218:1225	the suitable features	1205:1225	the suitable features of the CIP-loaded carrier	1205:1251	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	7	67	theme	carrier	1193:1199	arg1	properties					1155:1164	the beneficial properties	1140:1164	the beneficial properties of the chitosan-based drug carrier	1140:1199	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33607143	2	68	theme	classification	358:371	arg1	system					373:378	the biopharmaceutical classification system	336:378	the biopharmaceutical classification system	336:378	CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally.					
33607143	7	69	theme	suitable	1209:1216	arg1	features					1218:1225	the suitable features	1205:1225	the suitable features of the CIP-loaded carrier	1205:1251	Due to the beneficial properties of the chitosan-based drug carrier and the suitable features of the CIP-loaded carrier, this chitosan-based nano-drug delivery system can be regarded as an ideal candidate for oral delivery of the CIP as a drug model.					
33949265	0	0	theme	Roxb	82:85	arg1	kernels					49:55	the seed kernels	40:55	the seed kernels of Caesalpinia cucullata Roxb	40:85	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	4	1	theme	electronic	499:508	arg1	calculation					535:545	electronic circular dichroism (ECD) calculation	499:545	comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation	428:545	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	0	2	theme	cucullata	72:80	arg1	Roxb					82:85	Caesalpinia cucullata Roxb	60:85	Caesalpinia cucullata Roxb	60:85	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	1	3	theme	Caesalpinia	155:165	arg1	Roxb					177:180	Caesalpinia cucullata Roxb	155:180	Caesalpinia cucullata Roxb	155:180	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	5	4	theme	rare	573:576	arg1	ring					602:605	a rare isomerized dihydrofuran ring	571:605	a rare isomerized dihydrofuran ring in structure framework	571:628	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
33949265	6	5	theme	RAW	750:752	arg1	cells					760:764	LPS-induced RAW 264.7 cells	738:764	LPS-induced RAW 264.7 cells	738:764	In bioassay, compounds 1-4 exhibited moderate anti-inflammatory activity by inhibiting production of NO in LPS-induced RAW 264.7 cells.					
33949265	4	6	theme	dichroism	519:527	arg1	calculation					535:545	electronic circular dichroism (ECD) calculation	499:545	comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation	428:545	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	0	7	from	kernels	49:55	arg1	diterpenoids					22:33	Four new cassane-type diterpenoids	0:33	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.	0:86	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	5	8	theme	isomerized	578:587	arg1	ring					602:605	a rare isomerized dihydrofuran ring	571:605	a rare isomerized dihydrofuran ring in structure framework	571:628	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
33949265	6	9	theme	LPS-induced	738:748	arg1	cells					760:764	LPS-induced RAW 264.7 cells	738:764	LPS-induced RAW 264.7 cells	738:764	In bioassay, compounds 1-4 exhibited moderate anti-inflammatory activity by inhibiting production of NO in LPS-induced RAW 264.7 cells.					
33949265	4	10	theme	circular	510:517	arg1	ECD					530:532	ECD	530:532	ECD	530:532	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	4	10	theme	circular	510:517	arg1	dichroism					519:527	circular dichroism	510:527	comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation	428:545	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	1	11	theme	cucullata	167:175	arg1	Roxb					177:180	Caesalpinia cucullata Roxb	155:180	Caesalpinia cucullata Roxb	155:180	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	4	12	theme	spectroscopic	442:454	arg1	analysis					456:463	comprehensive spectroscopic analysis	428:463	comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation	428:545	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	6	13	theme	NO	732:733	arg1	production					718:727	production	718:727	production of NO in LPS-induced RAW 264.7 cells	718:764	In bioassay, compounds 1-4 exhibited moderate anti-inflammatory activity by inhibiting production of NO in LPS-induced RAW 264.7 cells.					
33949265	1	14	theme	Roxb	177:180	arg1	kernels					144:150	seed kernels	139:150	seed kernels of Caesalpinia cucullata Roxb	139:180	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	0	15	theme	new	5:7	arg1	diterpenoids					22:33	Four new cassane-type diterpenoids	0:33	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.	0:86	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	4	16	theme	comprehensive	428:440	arg1	analysis					456:463	comprehensive spectroscopic analysis	428:463	comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation	428:545	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	2	17	dep	furanoditerpenes	258:273	arg1	4					285:285	4	285:285	4	285:285	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	17	dep	furanoditerpenes	258:273	arg1	2					279:279	2	279:279	2	279:279	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	3	18	theme	found	333:337	arg1	diterpenoids					347:358	the first found cassane diterpenoids	323:358	the first found cassane diterpenoids from this plant	323:374	It was the first found cassane diterpenoids from this plant.					
33949265	3	18	theme	found	333:337	arg1	It					316:317	It	316:317	It	316:317	It was the first found cassane diterpenoids from this plant.					
33949265	1	19	theme	first	92:96	arg1	investigation					98:110	The first investigation	88:110	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.	88:181	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	6	20	from	production	718:727	arg1	cells					760:764	LPS-induced RAW 264.7 cells	738:764	LPS-induced RAW 264.7 cells	738:764	In bioassay, compounds 1-4 exhibited moderate anti-inflammatory activity by inhibiting production of NO in LPS-induced RAW 264.7 cells.					
33949265	0	21	theme	cassane-type	9:20	arg1	diterpenoids					22:33	Four new cassane-type diterpenoids	0:33	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.	0:86	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	2	22	theme	diterpenes	230:239	arg1	separation					199:208	the separation	195:208	the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3)	195:313	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	23	theme	cassane	222:228	arg1	type					306:309	one tricyclic type	292:309	one tricyclic type (3)	292:313	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	23	theme	cassane	222:228	arg1	diterpenes					230:239	four new cassane diterpenes	213:239	four new cassane diterpenes	213:239	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	23	theme	cassane	222:228	arg1	furanoditerpenes					258:273	three furanoditerpenes	252:273	three furanoditerpenes (1, 2 and 4)	252:286	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	3	24	theme	cassane	339:345	arg1	diterpenoids					347:358	the first found cassane diterpenoids	323:358	the first found cassane diterpenoids from this plant	323:374	It was the first found cassane diterpenoids from this plant.					
33949265	3	24	theme	cassane	339:345	arg1	It					316:317	It	316:317	It	316:317	It was the first found cassane diterpenoids from this plant.					
33949265	5	25	theme	dihydrofuran	589:600	arg1	ring					602:605	a rare isomerized dihydrofuran ring	571:605	a rare isomerized dihydrofuran ring in structure framework	571:628	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
33949265	2	26	theme	new	218:220	arg1	type					306:309	one tricyclic type	292:309	one tricyclic type (3)	292:313	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	26	theme	new	218:220	arg1	diterpenes					230:239	four new cassane diterpenes	213:239	four new cassane diterpenes	213:239	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	26	theme	new	218:220	arg1	furanoditerpenes					258:273	three furanoditerpenes	252:273	three furanoditerpenes (1, 2 and 4)	252:286	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	0	27	theme	seed	44:47	arg1	kernels					49:55	the seed kernels	40:55	the seed kernels of Caesalpinia cucullata Roxb	40:85	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	6	28	theme	anti-inflammatory	677:693	arg1	activity					695:702	moderate anti-inflammatory activity	668:702	moderate anti-inflammatory activity	668:702	In bioassay, compounds 1-4 exhibited moderate anti-inflammatory activity by inhibiting production of NO in LPS-induced RAW 264.7 cells.					
33949265	1	29	theme	chemical	115:122	arg1	composition					124:134	chemical composition	115:134	chemical composition	115:134	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	4	30	dep	analysis	456:463	arg1	NMR					474:476	NMR	474:476	NMR	474:476	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	4	30	dep	analysis	456:463	arg1	IR					470:471	IR	470:471	IR	470:471	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	4	30	dep	analysis	456:463	arg1	UV					466:467	UV	466:467	UV	466:467	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	4	30	dep	analysis	456:463	arg1	HRESIMS					479:485	HRESIMS	479:485	HRESIMS	479:485	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	6	31	theme	moderate	668:675	arg1	activity					695:702	moderate anti-inflammatory activity	668:702	moderate anti-inflammatory activity	668:702	In bioassay, compounds 1-4 exhibited moderate anti-inflammatory activity by inhibiting production of NO in LPS-induced RAW 264.7 cells.					
33949265	1	32	theme	composition	124:134	arg1	investigation					98:110	The first investigation	88:110	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.	88:181	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	1	33	from	investigation	98:110	arg1	kernels					144:150	seed kernels	139:150	seed kernels of Caesalpinia cucullata Roxb	139:180	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	5	34	theme	structure	610:618	arg1	framework					620:628	structure framework	610:628	structure framework	610:628	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
33949265	4	35	theme	gross	383:387	arg1	structures					389:398	Their gross structures	377:398	Their gross structures	377:398	Their gross structures were elucidated by means of comprehensive spectroscopic analysis (UV, IR, NMR, HRESIMS) as well as electronic circular dichroism (ECD) calculation.					
33949265	3	36	from	plant	370:374	arg1	diterpenoids					347:358	the first found cassane diterpenoids	323:358	the first found cassane diterpenoids from this plant	323:374	It was the first found cassane diterpenoids from this plant.					
33949265	3	36	from	plant	370:374	arg1	It					316:317	It	316:317	It	316:317	It was the first found cassane diterpenoids from this plant.					
33949265	2	37	theme	tricyclic	296:304	arg1	type					306:309	one tricyclic type	292:309	one tricyclic type (3)	292:313	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	2	37	theme	tricyclic	296:304	arg1	3					312:312	3	312:312	3	312:312	resulted in the separation of four new cassane diterpenes, including three furanoditerpenes (1, 2 and 4) and one tricyclic type (3).					
33949265	0	38	theme	Caesalpinia	60:70	arg1	Roxb					82:85	Caesalpinia cucullata Roxb	60:85	Caesalpinia cucullata Roxb	60:85	Four new cassane-type diterpenoids from the seed kernels of Caesalpinia cucullata Roxb.					
33949265	1	39	theme	seed	139:142	arg1	kernels					144:150	seed kernels	139:150	seed kernels of Caesalpinia cucullata Roxb	139:180	The first investigation of chemical composition in seed kernels of Caesalpinia cucullata Roxb.					
33949265	5	40	from	ring	602:605	arg1	framework					620:628	structure framework	610:628	structure framework	610:628	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
33949265	5	41	contain	possess	563:569	arg1	compound					552:559	The compound 1	548:561	The compound 1	548:561	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
33949265	5	41	contain	possess	563:569	arg2	ring					602:605	a rare isomerized dihydrofuran ring	571:605	a rare isomerized dihydrofuran ring in structure framework	571:628	The compound 1 possess a rare isomerized dihydrofuran ring in structure framework.					
34259534	5	0	theme	polydispersity	1217:1230	arg1	index					1232:1236	polydispersity index	1217:1236	polydispersity index (PI) < 0.2	1217:1247	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	0	theme	polydispersity	1217:1230	arg1	PI					1239:1240	PI	1239:1240	PI	1239:1240	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	4	1	theme	nasal	1088:1092	arg1	mucosa					1094:1099	the nasal mucosa	1084:1099	the nasal mucosa	1084:1099	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	9	2	theme	mucosal	2094:2100	arg1	permeation					2102:2111	fast mucosal permeation	2089:2111	fast mucosal permeation of the model drug, or (b) mucopenetration	2089:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	8	3	theme	simvastatin	1828:1838	arg1	transport					1840:1848	simvastatin transport	1828:1848	simvastatin transport across the epithelial barrier of the nasal cavity	1828:1898	In fact, all of the produced nanoparticles improved simvastatin transport across the epithelial barrier of the nasal cavity as compared to a traditional formulation.					
34259534	9	4	dep	mucopenetration	2139:2153	arg1	b					2136:2136	b	2136:2136	b	2136:2136	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	10	5	theme	platforms	2445:2453	arg1	development					2424:2434	the development	2420:2434	the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration	2420:2552	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	3	6	theme	poly-ε-caprolactone	695:713	arg1	nanocapsules					715:726	(iii) polymeric poly-ε-caprolactone nanocapsules	679:726	(iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH)	679:820	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	2	7	theme	exogenous	268:276	arg1	molecules					278:286	exogenous molecules	268:286	exogenous molecules	268:286	However, the possibility of reaching therapeutically relevant levels of exogenous molecules in the body is strongly reliant on the ability of the nanoparticles to overcome biological barriers.					
34259534	9	8	theme	model	2120:2124	arg1	drug					2126:2129	the model drug	2116:2129	the model drug	2116:2129	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	8	9	theme	epithelial	1861:1870	arg1	barrier					1872:1878	the epithelial barrier	1857:1878	the epithelial barrier of the nasal cavity	1857:1898	In fact, all of the produced nanoparticles improved simvastatin transport across the epithelial barrier of the nasal cavity as compared to a traditional formulation.					
34259534	3	10	dep	nanocapsules	596:607	arg1	ii					562:563	ii	562:563	ii	562:563	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	7	11	theme	mucus	1614:1618	arg1	layer					1620:1624	the mucus layer	1610:1624	the mucus layer	1610:1624	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	3	12	dep	hyaluronate	800:810	arg1	i.e.					779:782	i.e., sodium caproyl hyaluronate (PCL_SCH)	779:820	i.e.	779:782	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	1	13	theme	promising	111:119	arg1	mediators					121:129	promising mediators	111:129	promising mediators	111:129	Nanoparticles are promising mediators to enable nasal systemic and brain delivery of active compounds.					
34259534	1	13	theme	promising	111:119	arg1	Nanoparticles					93:105	Nanoparticles	93:105	Nanoparticles	93:105	Nanoparticles are promising mediators to enable nasal systemic and brain delivery of active compounds.					
34259534	9	14	with	nanocapsules	2231:2242	arg1	delayed					2249:2255	delayed	2249:2255	delayed	2249:2255	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	2	15	theme	biological	368:377	arg1	barriers					379:386	biological barriers	368:386	biological barriers	368:386	However, the possibility of reaching therapeutically relevant levels of exogenous molecules in the body is strongly reliant on the ability of the nanoparticles to overcome biological barriers.					
34259534	5	16	theme	drug	1260:1263	arg1	efficiency					1279:1288	high drug encapsulation efficiency	1255:1288	high drug encapsulation efficiency (>97%)	1255:1295	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	16	theme	drug	1260:1263	arg1	%					1294:1294	>97%	1291:1294	>97%	1291:1294	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	3	17	theme	sodium	785:790	arg1	PCL_SCH					813:819	PCL_SCH	813:819	PCL_SCH	813:819	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	17	theme	sodium	785:790	arg1	surfactant					767:776	a polysaccharide-based surfactant	744:776	a polysaccharide-based surfactant	744:776	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	17	theme	sodium	785:790	arg1	hyaluronate					800:810	sodium caproyl hyaluronate	785:810	sodium caproyl hyaluronate (PCL_SCH)	785:820	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	8	18	theme	nasal	1887:1891	arg1	cavity					1893:1898	the nasal cavity	1883:1898	the nasal cavity	1883:1898	In fact, all of the produced nanoparticles improved simvastatin transport across the epithelial barrier of the nasal cavity as compared to a traditional formulation.					
34259534	7	19	with	kinetics	1634:1641	arg1	layer					1620:1624	the mucus layer	1610:1624	the mucus layer	1610:1624	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	10	20	theme	pharmaceutical	2499:2512	arg1	compounds					2514:2522	pharmaceutical compounds	2499:2522	pharmaceutical compounds	2499:2522	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	4	21	from	impact	929:934	arg1	aspects					961:967	the biopharmaceutical aspects	939:967	the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa	939:1099	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	3	22	theme	polymeric	566:574	arg1	nanocapsules					596:607	(ii) polymeric poly-ε-caprolactone nanocapsules	561:607	(ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80)	561:672	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	23	dep	nanoparticles	539:551	arg1	i					511:511	i	511:511	i	511:511	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	7	24	theme	animal	1698:1703	arg1	epithelium					1711:1720	the excised animal nasal epithelium	1686:1720	the excised animal nasal epithelium	1686:1720	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	7	25	with	extent	1647:1652	arg1	layer					1620:1624	the mucus layer	1610:1624	the mucus layer	1610:1624	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	9	26	dep	delayed	2249:2255	arg1	boost					2257:2261	boost	2257:2261	boost	2257:2261	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	5	27	theme	encapsulation	1265:1277	arg1	efficiency					1279:1288	high drug encapsulation efficiency	1255:1288	high drug encapsulation efficiency (>97%)	1255:1295	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	27	theme	encapsulation	1265:1277	arg1	%					1294:1294	>97%	1291:1294	>97%	1291:1294	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	3	28	theme	nonionic	629:636	arg1	polysorbate					649:659	the nonionic surfactant polysorbate 80 (PCL_P80)	625:672	the nonionic surfactant polysorbate 80 (PCL_P80)	625:672	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	29	theme	model	471:475	arg1	simvastatin					482:492	the poorly soluble model drug simvastatin	452:492	the poorly soluble model drug simvastatin	452:492	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	9	30	theme	improved	2162:2169	arg1	uptake					2171:2176	an improved uptake	2159:2176	an improved uptake	2159:2176	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	8	31	theme	traditional	1917:1927	arg1	formulation					1929:1939	a traditional formulation	1915:1939	a traditional formulation	1915:1939	In fact, all of the produced nanoparticles improved simvastatin transport across the epithelial barrier of the nasal cavity as compared to a traditional formulation.					
34259534	7	32	theme	drug	1674:1677	arg1	transport					1657:1665	transport	1657:1665	transport of the drug across the excised animal nasal epithelium	1657:1720	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	5	33	dep	distribution	1203:1214	arg1	index					1232:1236	polydispersity index	1217:1236	polydispersity index (PI) < 0.2	1217:1247	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	33	dep	distribution	1203:1214	arg1	PI					1239:1240	PI	1239:1240	PI	1239:1240	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	34	theme	particle	1166:1173	arg1	nm					1186:1187	∼200 nm	1181:1187	∼200 nm	1181:1187	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	34	theme	particle	1166:1173	arg1	size					1175:1178	small particle size	1160:1178	small particle size (∼200 nm)	1160:1188	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	35	theme	high	1255:1258	arg1	efficiency					1279:1288	high drug encapsulation efficiency	1255:1288	high drug encapsulation efficiency (>97%)	1255:1295	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	35	theme	high	1255:1258	arg1	%					1294:1294	>97%	1291:1294	>97%	1291:1294	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	0	36	theme	Soluble	78:84	arg1	Drugs					86:90	Poorly Soluble Drugs	71:90	Poorly Soluble Drugs	71:90	Structure and Fate of Nanoparticles Designed for the Nasal Delivery of Poorly Soluble Drugs.					
34259534	9	37	theme	whole	2205:2209	arg1	PCL_P80					2211:2217	whole PCL_P80 and PCL_SCH nanocapsules	2205:2242	PCL_P80	2211:2217	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	7	38	with	interaction	1593:1603	arg1	layer					1620:1624	the mucus layer	1610:1624	the mucus layer	1610:1624	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	3	39	theme	paradigmatic	409:420	arg1	nanoformulations					422:437	three paradigmatic nanoformulations	403:437	three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin	403:492	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	8	40	theme	produced	1796:1803	arg1	nanoparticles					1805:1817	the produced nanoparticles	1792:1817	the produced nanoparticles	1792:1817	In fact, all of the produced nanoparticles improved simvastatin transport across the epithelial barrier of the nasal cavity as compared to a traditional formulation.					
34259534	9	41	theme	nanocapsules	2231:2242	arg1	mucoadhesion					2046:2057	mucoadhesion	2046:2057	mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration	2046:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	41	theme	nanocapsules	2231:2242	arg1	uptake					2171:2176	an improved uptake	2159:2176	an improved uptake	2159:2176	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	41	theme	nanocapsules	2231:2242	arg1	transport					2192:2200	potential transport	2182:2200	potential transport	2182:2200	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	5	42	theme	size	1198:1201	arg1	distribution					1203:1214	narrow size distribution	1191:1214	narrow size distribution (polydispersity index (PI) < 0.2)	1191:1248	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	3	43	theme	lecithin/chitosan	521:537	arg1	LCNs					554:557	LCNs	554:557	LCNs	554:557	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	43	theme	lecithin/chitosan	521:537	arg1	nanoparticles					539:551	(i) hybrid lecithin/chitosan nanoparticles	510:551	(i) hybrid lecithin/chitosan nanoparticles (LCNs)	510:558	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	6	44	from	impact	1490:1495	arg1	behavior					1551:1558	its behavior	1547:1558	its behavior	1547:1558	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	44	from	impact	1490:1495	arg1	profile					1522:1528	drug-release profile	1509:1528	drug-release profile	1509:1528	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	4	45	theme	drug	1034:1037	arg1	release					1039:1045	drug release	1034:1045	drug release	1034:1045	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	0	46	theme	Nanoparticles	22:34	arg1	Fate					14:17	Fate	14:17	Fate	14:17	Structure and Fate of Nanoparticles Designed for the Nasal Delivery of Poorly Soluble Drugs.					
34259534	0	46	theme	Nanoparticles	22:34	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Fate of Nanoparticles Designed for the Nasal Delivery of Poorly Soluble Drugs.					
34259534	0	47	theme	Nasal	53:57	arg1	Delivery					59:66	the Nasal Delivery	49:66	the Nasal Delivery of Poorly Soluble Drugs	49:90	Structure and Fate of Nanoparticles Designed for the Nasal Delivery of Poorly Soluble Drugs.					
34259534	9	48	dep	achieved	1997:2004	arg1	a					2034:2034	a	2034:2034	a	2034:2034	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	49	theme	hybrid	2063:2068	arg1	LCN					2070:2072	hybrid LCN	2063:2072	hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration	2063:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	50	theme	nasal	2288:2292	arg1	mucosa					2294:2299	the nasal mucosa	2284:2299	the nasal mucosa	2284:2299	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	3	51	theme	polymeric	685:693	arg1	nanocapsules					715:726	(iii) polymeric poly-ε-caprolactone nanocapsules	679:726	(iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH)	679:820	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	2	52	theme	nanoparticles	342:354	arg1	ability					327:333	the ability	323:333	the ability of the nanoparticles to overcome biological barriers	323:386	However, the possibility of reaching therapeutically relevant levels of exogenous molecules in the body is strongly reliant on the ability of the nanoparticles to overcome biological barriers.					
34259534	4	53	theme	physicochemical	873:887	arg1	properties					904:913	their physicochemical and structural properties	867:913	their physicochemical and structural properties	867:913	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	9	54	theme	fast	2089:2092	arg1	permeation					2102:2111	fast mucosal permeation	2089:2111	fast mucosal permeation of the model drug, or (b) mucopenetration	2089:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	6	55	theme	neutron	1435:1441	arg1	scattering					1443:1452	small-angle neutron scattering	1423:1452	small-angle neutron scattering	1423:1452	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	4	56	theme	structural	893:902	arg1	properties					904:913	their physicochemical and structural properties	867:913	their physicochemical and structural properties	867:913	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	7	57	theme	nanoparticle	1740:1751	arg1	structure					1753:1761	nanoparticle structure	1740:1761	nanoparticle structure	1740:1761	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	2	58	theme	molecules	278:286	arg1	levels					258:263	therapeutically relevant levels	233:263	therapeutically relevant levels of exogenous molecules	233:286	However, the possibility of reaching therapeutically relevant levels of exogenous molecules in the body is strongly reliant on the ability of the nanoparticles to overcome biological barriers.					
34259534	6	59	theme	drug-release	1509:1520	arg1	profile					1522:1528	drug-release profile	1509:1528	drug-release profile	1509:1528	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	1	60	theme	brain	160:164	arg1	delivery					166:173	nasal systemic and brain delivery	141:173	delivery	166:173	Nanoparticles are promising mediators to enable nasal systemic and brain delivery of active compounds.					
34259534	10	61	theme	pivotal	2402:2408	arg1	point					2410:2414	a pivotal point	2400:2414	a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration	2400:2552	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	10	61	theme	pivotal	2402:2408	arg1	correlation					2306:2316	The correlation	2302:2316	The correlation between nanoparticle structure and its biopharmaceutical properties	2302:2384	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	10	62	theme	novel	2439:2443	arg1	platforms					2445:2453	novel platforms	2439:2453	novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration	2439:2552	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	6	63	dep	profile	1522:1528	arg1	the					1505:1507	the	1505:1507	the	1505:1507	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	9	64	theme	drug	2126:2129	arg1	permeation					2102:2111	fast mucosal permeation	2089:2111	fast mucosal permeation of the model drug, or (b) mucopenetration	2089:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	6	65	theme	surface	1324:1330	arg1	charge					1332:1337	surface charge	1324:1337	surface charge	1324:1337	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	1	66	theme	compounds	185:193	arg1	systemic					147:154	systemic	147:154	systemic	147:154	Nanoparticles are promising mediators to enable nasal systemic and brain delivery of active compounds.					
34259534	10	67	theme	systemic	2468:2475	arg1	delivery					2487:2494	systemic and brain delivery	2468:2494	delivery	2487:2494	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	3	68	theme	polysaccharide-based	746:765	arg1	surfactant					767:776	a polysaccharide-based surfactant	744:776	a polysaccharide-based surfactant	744:776	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	68	theme	polysaccharide-based	746:765	arg1	hyaluronate					800:810	sodium caproyl hyaluronate	785:810	sodium caproyl hyaluronate (PCL_SCH)	785:820	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	8	69	theme	cavity	1893:1898	arg1	barrier					1872:1878	the epithelial barrier	1857:1878	the epithelial barrier of the nasal cavity	1857:1898	In fact, all of the produced nanoparticles improved simvastatin transport across the epithelial barrier of the nasal cavity as compared to a traditional formulation.					
34259534	3	70	theme	poly-ε-caprolactone	576:594	arg1	nanocapsules					596:607	(ii) polymeric poly-ε-caprolactone nanocapsules	561:607	(ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80)	561:672	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	1	71	theme	active	178:183	arg1	compounds					185:193	active compounds	178:193	active compounds	178:193	Nanoparticles are promising mediators to enable nasal systemic and brain delivery of active compounds.					
34259534	4	72	theme	biopharmaceutical	943:959	arg1	aspects					961:967	the biopharmaceutical aspects	939:967	the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa	939:1099	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	10	73	theme	suitable	2455:2462	arg1	platforms					2445:2453	novel platforms	2439:2453	novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration	2439:2552	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	3	74	theme	caproyl	792:798	arg1	PCL_SCH					813:819	PCL_SCH	813:819	PCL_SCH	813:819	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	74	theme	caproyl	792:798	arg1	surfactant					767:776	a polysaccharide-based surfactant	744:776	a polysaccharide-based surfactant	744:776	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	74	theme	caproyl	792:798	arg1	hyaluronate					800:810	sodium caproyl hyaluronate	785:810	sodium caproyl hyaluronate (PCL_SCH)	785:820	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	6	75	theme	internal	1344:1351	arg1	assessed					1411:1418	assessed	1411:1418	assessed by small-angle neutron scattering	1411:1452	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	75	theme	internal	1344:1351	arg1	structure					1353:1361	internal structure	1344:1361	internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS)	1344:1459	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	5	76	with	reproducible	1141:1152	arg1	nm					1186:1187	∼200 nm	1181:1187	∼200 nm	1181:1187	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	76	with	reproducible	1141:1152	arg1	size					1175:1178	small particle size	1160:1178	small particle size (∼200 nm)	1160:1188	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	76	with	reproducible	1141:1152	arg1	efficiency					1279:1288	high drug encapsulation efficiency	1255:1288	high drug encapsulation efficiency (>97%)	1255:1295	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	76	with	reproducible	1141:1152	arg1	%					1294:1294	>97%	1291:1294	>97%	1291:1294	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	76	with	reproducible	1141:1152	arg1	distribution					1203:1214	narrow size distribution	1191:1214	narrow size distribution (polydispersity index (PI) < 0.2)	1191:1248	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	9	77	theme	mucopenetration	2139:2153	arg1	permeation					2102:2111	fast mucosal permeation	2089:2111	fast mucosal permeation of the model drug, or (b) mucopenetration	2089:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	7	78	theme	nasal	1705:1709	arg1	epithelium					1711:1720	the excised animal nasal epithelium	1686:1720	the excised animal nasal epithelium	1686:1720	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	9	79	with	PCL_P80	2211:2217	arg1	delayed					2249:2255	delayed	2249:2255	delayed	2249:2255	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	10	80	theme	brain	2481:2485	arg1	delivery					2487:2494	systemic and brain delivery	2468:2494	delivery	2487:2494	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	2	81	theme	relevant	249:256	arg1	levels					258:263	therapeutically relevant levels	233:263	therapeutically relevant levels of exogenous molecules	233:286	However, the possibility of reaching therapeutically relevant levels of exogenous molecules in the body is strongly reliant on the ability of the nanoparticles to overcome biological barriers.					
34259534	7	82	theme	excised	1690:1696	arg1	epithelium					1711:1720	the excised animal nasal epithelium	1686:1720	the excised animal nasal epithelium	1686:1720	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	4	83	theme	nose-to-brain	992:1004	arg1	delivery					1006:1013	nasal and nose-to-brain delivery	982:1013	nasal and nose-to-brain delivery	982:1013	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	10	84	theme	intranasal	2528:2537	arg1	administration					2539:2552	intranasal administration	2528:2552	intranasal administration	2528:2552	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	10	85	theme	biopharmaceutical	2357:2373	arg1	properties					2375:2384	its biopharmaceutical properties	2353:2384	its biopharmaceutical properties	2353:2384	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	5	86	theme	small	1160:1164	arg1	nm					1186:1187	∼200 nm	1181:1187	∼200 nm	1181:1187	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	5	86	theme	small	1160:1164	arg1	size					1175:1178	small particle size	1160:1178	small particle size (∼200 nm)	1160:1188	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	9	87	theme	permeation	1970:1979	arg1	enhancement					1981:1991	the permeation enhancement	1966:1991	the permeation enhancement	1966:1991	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	10	88	theme	compounds	2514:2522	arg1	delivery					2487:2494	systemic and brain delivery	2468:2494	delivery	2487:2494	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	9	89	theme	potential	2182:2190	arg1	transport					2192:2200	potential transport	2182:2200	potential transport	2182:2200	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	0	90	theme	Drugs	86:90	arg1	Delivery					59:66	the Nasal Delivery	49:66	the Nasal Delivery of Poorly Soluble Drugs	49:90	Structure and Fate of Nanoparticles Designed for the Nasal Delivery of Poorly Soluble Drugs.					
34259534	4	91	theme	critical	969:976	arg1	aspects					961:967	the biopharmaceutical aspects	939:967	the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa	939:1099	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	6	92	contain	have	1482:1485	arg1	composition					1311:1321	Nanoparticle composition	1298:1321	Nanoparticle composition	1298:1321	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	92	contain	have	1482:1485	arg1	charge					1332:1337	surface charge	1324:1337	surface charge	1324:1337	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	92	contain	have	1482:1485	arg1	assessed					1411:1418	assessed	1411:1418	assessed by small-angle neutron scattering	1411:1452	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	92	contain	have	1482:1485	arg1	structure					1353:1361	internal structure	1344:1361	internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS)	1344:1459	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	92	contain	have	1482:1485	arg2	impact					1490:1495	an impact	1487:1495	an impact on both the drug-release profile and, strikingly, its behavior	1487:1558	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	3	93	dep	addressed	499:507	arg1	nanocapsules					715:726	(iii) polymeric poly-ε-caprolactone nanocapsules	679:726	(iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH)	679:820	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	93	dep	addressed	499:507	arg1	nanocapsules					596:607	(ii) polymeric poly-ε-caprolactone nanocapsules	561:607	(ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80)	561:672	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	93	dep	addressed	499:507	arg1	nanoparticles					539:551	(i) hybrid lecithin/chitosan nanoparticles	510:551	(i) hybrid lecithin/chitosan nanoparticles (LCNs)	510:558	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	93	dep	addressed	499:507	arg1	LCNs					554:557	LCNs	554:557	LCNs	554:557	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	7	94	theme	transport	1657:1665	arg1	extent					1647:1652	extent	1647:1652	extent	1647:1652	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	7	94	theme	transport	1657:1665	arg1	kinetics					1634:1641	kinetics	1634:1641	kinetics	1634:1641	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	7	94	theme	transport	1657:1665	arg1	interaction					1593:1603	The interaction	1589:1603	The interaction with the mucus layer	1589:1624	The interaction with the mucus layer and the kinetics and extent of transport of the drug across the excised animal nasal epithelium were modulated by nanoparticle structure and surface.					
34259534	4	95	theme	nasal	982:986	arg1	delivery					1006:1013	nasal and nose-to-brain delivery	982:1013	nasal and nose-to-brain delivery	982:1013	The three nanosystems were investigated for their physicochemical and structural properties and for their impact on the biopharmaceutical aspects critical for nasal and nose-to-brain delivery: biocompatibility, drug release, mucoadhesion, and permeation across the nasal mucosa.					
34259534	9	96	theme	PCL_P80	2211:2217	arg1	mucoadhesion					2046:2057	mucoadhesion	2046:2057	mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration	2046:2153	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	96	theme	PCL_P80	2211:2217	arg1	uptake					2171:2176	an improved uptake	2159:2176	an improved uptake	2159:2176	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	96	theme	PCL_P80	2211:2217	arg1	transport					2192:2200	potential transport	2182:2200	potential transport	2182:2200	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	6	97	theme	small-angle	1423:1433	arg1	scattering					1443:1452	small-angle neutron scattering	1423:1452	small-angle neutron scattering	1423:1452	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	9	98	theme	distinct	2014:2021	arg1	pathways					2023:2030	two distinct pathways	2010:2030	two distinct pathways	2010:2030	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	9	99	theme	PCL_SCH	2223:2229	arg1	nanocapsules					2231:2242	whole PCL_P80 and PCL_SCH nanocapsules	2205:2242	nanocapsules	2231:2242	Interestingly, however, the permeation enhancement was achieved via two distinct pathways: (a) enhanced mucoadhesion for hybrid LCN accompanied by fast mucosal permeation of the model drug, or (b) mucopenetration and an improved uptake and potential transport of whole PCL_P80 and PCL_SCH nanocapsules with delayed boost of permeation across the nasal mucosa.					
34259534	5	100	theme	narrow	1191:1196	arg1	distribution					1203:1214	narrow size distribution	1191:1214	narrow size distribution (polydispersity index (PI) < 0.2)	1191:1248	All three nanoformulations were highly reproducible, with small particle size (∼200 nm), narrow size distribution (polydispersity index (PI) < 0.2), and high drug encapsulation efficiency (>97%).					
34259534	6	101	theme	biological	1567:1576	arg1	interface					1578:1586	the biological interface	1563:1586	the biological interface	1563:1586	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	3	102	theme	drug	477:480	arg1	simvastatin					482:492	the poorly soluble model drug simvastatin	452:492	the poorly soluble model drug simvastatin	452:492	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	103	theme	surfactant	638:647	arg1	polysorbate					649:659	the nonionic surfactant polysorbate 80 (PCL_P80)	625:672	the nonionic surfactant polysorbate 80 (PCL_P80)	625:672	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	104	theme	soluble	463:469	arg1	simvastatin					482:492	the poorly soluble model drug simvastatin	452:492	the poorly soluble model drug simvastatin	452:492	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	105	dep	nanocapsules	715:726	arg1	iii					680:682	iii	680:682	iii	680:682	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	6	106	dep	assessed	1411:1418	arg1	raspberry-like					1392:1405	raspberry-like	1392:1405	raspberry-like	1392:1405	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	106	dep	assessed	1411:1418	arg1	core-shell					1378:1387	core-shell	1378:1387	core-shell	1378:1387	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	106	dep	assessed	1411:1418	arg1	SANS					1455:1458	SANS	1455:1458	SANS	1455:1458	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	106	dep	assessed	1411:1418	arg1	multilayered					1364:1375	multilayered	1364:1375	multilayered	1364:1375	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	6	107	theme	Nanoparticle	1298:1309	arg1	composition					1311:1321	Nanoparticle composition	1298:1321	Nanoparticle composition	1298:1321	Nanoparticle composition, surface charge, and internal structure (multilayered, core-shell or raspberry-like, as assessed by small-angle neutron scattering, SANS) were demonstrated to have an impact on both the drug-release profile and, strikingly, its behavior at the biological interface.					
34259534	10	108	theme	nanoparticle	2326:2337	arg1	structure					2339:2347	nanoparticle structure	2326:2347	nanoparticle structure	2326:2347	The correlation between nanoparticle structure and its biopharmaceutical properties appears to be a pivotal point for the development of novel platforms suitable for systemic and brain delivery of pharmaceutical compounds via intranasal administration.					
34259534	3	109	theme	hybrid	514:519	arg1	LCNs					554:557	LCNs	554:557	LCNs	554:557	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
34259534	3	109	theme	hybrid	514:519	arg1	nanoparticles					539:551	(i) hybrid lecithin/chitosan nanoparticles	510:551	(i) hybrid lecithin/chitosan nanoparticles (LCNs)	510:558	In this work, three paradigmatic nanoformulations vehiculating the poorly soluble model drug simvastatin were addressed: (i) hybrid lecithin/chitosan nanoparticles (LCNs), (ii) polymeric poly-ε-caprolactone nanocapsules stabilized with the nonionic surfactant polysorbate 80 (PCL_P80), and (iii) polymeric poly-ε-caprolactone nanocapsules stabilized with a polysaccharide-based surfactant, i.e., sodium caproyl hyaluronate (PCL_SCH).					
32544577	2	0	theme	-blended	486:493	arg1	scaffolds					548:556	Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds	448:556	Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer	448:602	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	1	theme	diabetic	589:596	arg1	ulcer					598:602	diabetic ulcer	589:602	diabetic ulcer	589:602	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	0	2	theme	3D-printing	100:110	arg1	technology					112:121	3D-printing technology	100:121	3D-printing technology	100:121	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	7	3	theme	antimicrobial	1066:1078	arg1	analysis					1080:1087	The antimicrobial analysis	1062:1087	The antimicrobial analysis	1062:1087	The antimicrobial analysis was evaluated against Escherichia coli and Staphylococcus aureus strains.					
32544577	10	4	theme	SA/PEG/SC	1453:1461	arg1	scaffolds					1463:1471	The SA/PEG/SC scaffolds	1449:1471	The SA/PEG/SC scaffolds	1449:1471	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	2	5	with	-blended	486:493	arg1	PEG					543:545	PEG	543:545	PEG	543:545	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	5	with	-blended	486:493	arg1	glycol					535:540	sodium alginate (SA) /polyethylene glycol	500:540	sodium alginate (SA) /polyethylene glycol (PEG)	500:546	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	1	6	theme	common	181:186	arg1	problem					195:201	a common health problem	179:201	a common health problem	179:201	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	1	6	theme	common	181:186	arg1	wounds					168:173	Acute wounds	162:173	Acute wounds	162:173	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	4	7	theme	scanning	821:828	arg1	SEM					851:853	SEM	851:853	SEM	851:853	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	4	7	theme	scanning	821:828	arg1	microscopy					839:848	scanning electron microscopy	821:848	scanning electron microscopy (SEM)	821:854	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	5	8	theme	degradation	972:982	arg1	behaviours					984:993	degradation behaviours	972:993	degradation behaviours	972:993	Mechanical and thermal properties, swelling, and degradation behaviours were also investigated.					
32544577	2	9	theme	SA	517:518	arg1	PEG					543:545	PEG	543:545	PEG	543:545	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	9	theme	SA	517:518	arg1	glycol					535:540	sodium alginate (SA) /polyethylene glycol	500:540	sodium alginate (SA) /polyethylene glycol (PEG)	500:546	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	1	10	theme	acute	366:370	arg1	healing					378:384	acute wound healing	366:384	acute wound healing around the globe	366:401	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	2	11	theme	potential	566:574	arg1	treatment					576:584	the potential treatment	562:584	the potential treatment of diabetic ulcer	562:602	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	8	12	dep	bacteria	1263:1270	arg1	contain					1313:1319	contain	1313:1319	contain	1313:1319	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	8	13	theme	antibacterial	1283:1295	arg1	extract					1300:1306	the antibacterial SC extract	1279:1306	the antibacterial SC extract	1279:1306	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	0	14	theme	wound	137:141	arg1	material					152:159	a diabetic wound dressing material	126:159	a diabetic wound dressing material	126:159	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	1	15	theme	wound	372:376	arg1	healing					378:384	acute wound healing	366:384	acute wound healing around the globe	366:401	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	4	16	theme	scaffolds	790:798	arg1	composition					740:750	The morphological and chemical composition	709:750	The morphological and chemical composition of the resulting 3D printed composite scaffolds	709:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	4	17	theme	morphological	713:725	arg1	composition					740:750	The morphological and chemical composition	709:750	The morphological and chemical composition of the resulting 3D printed composite scaffolds	709:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	9	18	theme	MTT	1438:1440	arg1	assay					1442:1446	the colourimetric MTT assay	1420:1446	the colourimetric MTT assay	1420:1446	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	1	19	theme	health	188:193	arg1	problem					195:201	a common health problem	179:201	a common health problem	179:201	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	1	19	theme	health	188:193	arg1	wounds					168:173	Acute wounds	162:173	Acute wounds	162:173	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	0	20	theme	diabetic	128:135	arg1	material					152:159	a diabetic wound dressing material	126:159	a diabetic wound dressing material	126:159	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	4	21	theme	composite	780:788	arg1	scaffolds					790:798	the resulting 3D printed composite scaffolds	755:798	the resulting 3D printed composite scaffolds	755:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	4	22	dep	transforms	868:877	arg1	infrared					879:886	infrared	879:886	infrared	879:886	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	2	23	theme	cuneifolia	457:466	arg1	extract					474:480	Satureja cuneifolia plant extract	448:480	Satureja cuneifolia plant extract (SC)	448:485	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	23	theme	cuneifolia	457:466	arg1	SC					483:484	SC	483:484	SC	483:484	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	10	24	theme	wound	1529:1533	arg1	healing					1535:1541	diabetic wound healing	1520:1541	diabetic wound healing	1520:1541	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	6	25	theme	release	1023:1029	arg1	kinetics					1031:1038	The release kinetics	1019:1038	The release kinetics of SC	1019:1044	The release kinetics of SC were performed.					
32544577	2	26	theme	Satureja	448:455	arg1	extract					474:480	Satureja cuneifolia plant extract	448:480	Satureja cuneifolia plant extract (SC)	448:485	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	26	theme	Satureja	448:455	arg1	SC					483:484	SC	483:484	SC	483:484	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	3	27	theme	9 wt	699:702	arg1	SA					705:706	9 wt% SA	699:706	9 wt% SA	699:706	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	2	28	theme	ulcer	598:602	arg1	treatment					576:584	the potential treatment	562:584	the potential treatment of diabetic ulcer	562:602	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	9	29	theme	on/within	1381:1389	arg1	scaffolds					1391:1399	on/within scaffolds	1381:1399	fibroblast (L929) cells on/within scaffolds	1357:1399	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	0	30	theme	cuneifolia-loaded	24:40	arg1	glycol					71:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	3	31	theme	%	703:703	arg1	SA					705:706	9 wt% SA	699:706	9 wt% SA	699:706	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	10	32	theme	promising	1486:1494	arg1	candidate					1506:1514	a great promising potential candidate	1478:1514	a great promising potential candidate for diabetic wound healing and against bacterial infections	1478:1574	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	4	33	theme	resulting	759:767	arg1	scaffolds					790:798	the resulting 3D printed composite scaffolds	755:798	the resulting 3D printed composite scaffolds	755:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	8	34	theme	gram-positive	1249:1261	arg1	bacteria					1263:1270	gram-positive bacteria due to the antibacterial SC extract they contain	1249:1319	gram-positive bacteria due to the antibacterial SC extract they contain	1249:1319	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	0	35	theme	Satureja	15:22	arg1	glycol					71:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	3	36	theme	PEG	692:694	arg1	concentrations					656:669	different concentrations	646:669	different concentrations (1, 3, and 5 wt%) of PEG	646:694	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	3	36	theme	PEG	692:694	arg1	%					686:686	1, 3, and 5 wt%	672:686	1, 3, and 5 wt%	672:686	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	0	37	theme	dressing	143:150	arg1	material					152:159	a diabetic wound dressing material	126:159	a diabetic wound dressing material	126:159	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	9	38	dep	cells	1375:1379	arg1	scaffolds					1391:1399	on/within scaffolds	1381:1399	fibroblast (L929) cells on/within scaffolds	1357:1399	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	6	39	theme	SC	1043:1044	arg1	kinetics					1031:1038	The release kinetics	1019:1038	The release kinetics of SC	1019:1044	The release kinetics of SC were performed.					
32544577	0	40	theme	alginate/polyethylene	49:69	arg1	glycol					71:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	9	41	theme	cell	1339:1342	arg1	viability					1344:1352	the cell viability	1335:1352	the cell viability of fibroblast (L929) cells on/within scaffolds	1335:1399	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	0	42	dep	scaffolds	78:86	arg1	produced					88:95	produced	88:95	scaffolds produced by 3D-printing technology as a diabetic wound dressing material	78:159	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	1	43	theme	people	221:226	arg1	millions					209:216	millions	209:216	millions of people	209:226	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	0	44	theme	sodium	42:47	arg1	glycol					71:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	15:76	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	1	45	theme	big	314:316	arg1	challenge					318:326	a big challenge	312:326	a big challenge for wound care researchers to promote acute wound healing around the globe	312:401	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	1	45	theme	big	314:316	arg1	it					301:302	it	301:302	it	301:302	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	2	46	theme	/polyethylene	521:533	arg1	PEG					543:545	PEG	543:545	PEG	543:545	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	46	theme	/polyethylene	521:533	arg1	glycol					535:540	sodium alginate (SA) /polyethylene glycol	500:540	sodium alginate (SA) /polyethylene glycol (PEG)	500:546	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	9	47	theme	cells	1375:1379	arg1	viability					1344:1352	the cell viability	1335:1352	the cell viability of fibroblast (L929) cells on/within scaffolds	1335:1399	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	2	48	theme	alginate	507:514	arg1	PEG					543:545	PEG	543:545	PEG	543:545	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	48	theme	alginate	507:514	arg1	glycol					535:540	sodium alginate (SA) /polyethylene glycol	500:540	sodium alginate (SA) /polyethylene glycol (PEG)	500:546	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	8	49	theme	excellent	1198:1206	arg1	effect					1222:1227	an excellent antibacterial effect	1195:1227	an excellent antibacterial effect	1195:1227	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	9	50	theme	colourimetric	1424:1436	arg1	assay					1442:1446	the colourimetric MTT assay	1420:1446	the colourimetric MTT assay	1420:1446	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	10	51	theme	diabetic	1520:1527	arg1	healing					1535:1541	diabetic wound healing	1520:1541	diabetic wound healing	1520:1541	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	4	52	theme	printed	772:778	arg1	scaffolds					790:798	the resulting 3D printed composite scaffolds	755:798	the resulting 3D printed composite scaffolds	755:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	0	53	theme	glycol	71:76	arg1	Development					0:10	Development	0:10	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol	0:76	Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material.					
32544577	9	54	theme	fibroblast	1357:1366	arg1	cells					1375:1379	fibroblast (L929) cells	1357:1379	fibroblast (L929) cells on/within scaffolds	1357:1399	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	2	55	theme	plant	468:472	arg1	extract					474:480	Satureja cuneifolia plant extract	448:480	Satureja cuneifolia plant extract (SC)	448:485	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	55	theme	plant	468:472	arg1	SC					483:484	SC	483:484	SC	483:484	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	4	56	theme	3D	769:770	arg1	scaffolds					790:798	the resulting 3D printed composite scaffolds	755:798	the resulting 3D printed composite scaffolds	755:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	10	57	theme	bacterial	1555:1563	arg1	infections					1565:1574	bacterial infections	1555:1574	bacterial infections	1555:1574	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	1	58	theme	wound	332:336	arg1	care					338:341	wound care	332:341	wound care	332:341	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	4	59	theme	chemical	731:738	arg1	composition					740:750	The morphological and chemical composition	709:750	The morphological and chemical composition of the resulting 3D printed composite scaffolds	709:798	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	3	60	theme	SA/PEG	605:610	arg1	scaffolds					612:620	SA/PEG scaffolds	605:620	SA/PEG scaffolds	605:620	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	8	61	theme	SC	1297:1298	arg1	extract					1300:1306	the antibacterial SC extract	1279:1306	the antibacterial SC extract	1279:1306	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	10	62	theme	great	1480:1484	arg1	candidate					1506:1514	a great promising potential candidate	1478:1514	a great promising potential candidate for diabetic wound healing and against bacterial infections	1478:1574	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	9	63	theme	L929	1369:1372	arg1	cells					1375:1379	fibroblast (L929) cells	1357:1379	fibroblast (L929) cells on/within scaffolds	1357:1399	Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay.					
32544577	10	64	theme	potential	1496:1504	arg1	candidate					1506:1514	a great promising potential candidate	1478:1514	a great promising potential candidate for diabetic wound healing and against bacterial infections	1478:1574	The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections.					
32544577	3	65	theme	different	646:654	arg1	concentrations					656:669	different concentrations	646:669	different concentrations (1, 3, and 5 wt%) of PEG	646:694	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	3	65	theme	different	646:654	arg1	%					686:686	1, 3, and 5 wt%	672:686	1, 3, and 5 wt%	672:686	SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA.					
32544577	4	66	dep	using	815:819	arg1	transforms					868:877	transforms	868:877	using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively	815:920	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	1	67	theme	Acute	162:166	arg1	problem					195:201	a common health problem	179:201	a common health problem	179:201	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	1	67	theme	Acute	162:166	arg1	wounds					168:173	Acute wounds	162:173	Acute wounds	162:173	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	4	68	theme	electron	830:837	arg1	SEM					851:853	SEM	851:853	SEM	851:853	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	4	68	theme	electron	830:837	arg1	microscopy					839:848	scanning electron microscopy	821:848	scanning electron microscopy (SEM)	821:854	The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively.					
32544577	1	69	theme	decreased	241:249	arg1	formation					270:278	decreased granulation tissue formation	241:278	decreased granulation tissue formation	241:278	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	2	70	theme	sodium	500:505	arg1	PEG					543:545	PEG	543:545	PEG	543:545	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	2	70	theme	sodium	500:505	arg1	glycol					535:540	sodium alginate (SA) /polyethylene glycol	500:540	sodium alginate (SA) /polyethylene glycol (PEG)	500:546	This study aims to produce and characterize Satureja cuneifolia plant extract (SC)-blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer.					
32544577	5	71	theme	Mechanical	923:932	arg1	properties					946:955	properties	946:955	properties	946:955	Mechanical and thermal properties, swelling, and degradation behaviours were also investigated.					
32544577	7	72	dep	coli	1123:1126	arg1	strains					1154:1160	strains	1154:1160	strains	1154:1160	The antimicrobial analysis was evaluated against Escherichia coli and Staphylococcus aureus strains.					
32544577	8	73	theme	antibacterial	1208:1220	arg1	effect					1222:1227	an excellent antibacterial effect	1195:1227	an excellent antibacterial effect	1195:1227	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	1	74	theme	granulation	251:261	arg1	formation					270:278	decreased granulation tissue formation	241:278	decreased granulation tissue formation	241:278	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
32544577	5	75	theme	thermal	938:944	arg1	properties					946:955	properties	946:955	properties	946:955	Mechanical and thermal properties, swelling, and degradation behaviours were also investigated.					
32544577	8	76	theme	printed	1166:1172	arg1	scaffolds					1174:1182	3D printed scaffolds	1163:1182	3D printed scaffolds	1163:1182	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	8	77	theme	3D	1163:1164	arg1	scaffolds					1174:1182	3D printed scaffolds	1163:1182	3D printed scaffolds	1163:1182	3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain.					
32544577	1	78	theme	tissue	263:268	arg1	formation					270:278	decreased granulation tissue formation	241:278	decreased granulation tissue formation	241:278	Acute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe.					
33989619	6	0	theme	Embelia	949:955	arg1	tsjeriam-cottam					957:971	Embelia tsjeriam-cottam	949:971	Embelia tsjeriam-cottam	949:971	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	6	1	theme	curve	1131:1135	arg1	analysis					1137:1144	time-kill curve analysis	1121:1144	time-kill curve analysis	1121:1144	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	10	2	theme	related	1847:1853	arg1	proteins					1855:1862	related proteins	1847:1862	related proteins	1847:1862	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	2	3	theme	nosocomial	361:370	arg1	environments					372:383	nosocomial environments	361:383	nosocomial environments	361:383	Unfortunately, ABR is no longer confined to nosocomial environments and is frequently reported from community microbes as well.					
33989619	5	4	theme	strains	852:858	arg1	profiling					833:841	ABR profiling	829:841	ABR profiling of these strains	829:858	ABR profiling of these strains characterized them as multidrug resistant (MDR).					
33989619	9	5	theme	increase	1626:1633	arg1	terms					1613:1617	terms	1613:1617	terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains	1613:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	10	6	theme	amino	1996:2000	arg1	residues					2007:2014	respective amino acid residues	1985:2014	respective amino acid residues	1985:2014	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	4	7	attach	isolated	801:808	arg1	waters					821:826	river waters	815:826	river waters	815:826	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	4	7	attach	isolated	801:808	arg2	PA					771:772	PA	771:772	PA	771:772	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	4	7	attach	isolated	801:808	arg2	aeruginosa					759:768	Pseudomonas aeruginosa	747:768	Pseudomonas aeruginosa (PA)	747:773	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	10	8	theme	in	1735:1736	arg1	simulations					1745:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	9	theme	docking	1721:1727	arg1	simulations					1745:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	1	10	theme	resistance	237:246	arg1	spread					216:221	spread	216:221	spread	216:221	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	1	10	theme	resistance	237:246	arg1	emergence					202:210	emergence	202:210	emergence	202:210	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	9	11	theme	mediated	1647:1654	arg1	fluorescence					1656:1667	the EtBr mediated fluorescence	1638:1667	the EtBr mediated fluorescence in control versus NP-treated MDR strains	1638:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	4	12	theme	metallic	710:717	arg1	a					647:647	a	647:647	a	647:647	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	4	12	theme	metallic	710:717	arg1	NPs					734:736	NPs	734:736	NPs	734:736	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	4	12	theme	metallic	710:717	arg1	nanoparticles					719:731	phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles	662:731	phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs)	662:737	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	9	13	from	terms	1613:1617	arg1	fluorescence					1656:1667	the EtBr mediated fluorescence	1638:1667	the EtBr mediated fluorescence in control versus NP-treated MDR strains	1638:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	8	14	theme	½	1446:1446	arg1	MICs					1448:1451	½ MICs	1446:1451	½ MICs in both PA-r and EC-r	1446:1473	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	15	theme	Fractional	1290:1299	arg1	concentration					1312:1324	Fractional inhibitory concentration	1290:1324	Fractional inhibitory concentration (FIC) indices with ≤0.5 values	1290:1355	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	15	theme	Fractional	1290:1299	arg1	FIC					1327:1329	FIC	1327:1329	FIC	1327:1329	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	1	16	from	spread	216:221	arg1	populations					267:277	bacterial populations	257:277	bacterial populations	257:277	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	9	17	theme	MDR	1698:1700	arg1	strains					1702:1708	control versus NP-treated MDR strains	1672:1708	control versus NP-treated MDR strains	1672:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	8	18	theme	concentration	1312:1324	arg1	indices					1332:1338	Fractional inhibitory concentration (FIC) indices	1290:1338	Fractional inhibitory concentration (FIC) indices with ≤0.5 values	1290:1355	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	19	with	indices	1332:1338	arg1	values					1350:1355	≤0.5 values	1345:1355	≤0.5 values	1345:1355	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	1	20	from	emergence	202:210	arg1	populations					267:277	bacterial populations	257:277	bacterial populations	257:277	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	1	21	theme	global	184:189	arg1	upsurge					191:197	A global upsurge	182:197	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations	182:277	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	1	21	theme	global	184:189	arg1	threat					292:297	a serious threat	282:297	a serious threat for human health	282:314	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	6	22	theme	potential	1037:1045	arg1	activity					1059:1066	their potential synergistic activity	1031:1066	their potential synergistic activity with ciprofloxacin (CIP)	1031:1091	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	0	23	theme	efflux	101:106	arg1	pumps					108:112	efflux pumps	101:112	efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli	101:179	Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.					
33989619	1	24	from	upsurge	191:197	arg1	spread					216:221	spread	216:221	spread	216:221	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	1	24	from	upsurge	191:197	arg1	emergence					202:210	emergence	202:210	emergence	202:210	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	9	25	from	fluorescence	1656:1667	arg1	terms					1613:1617	terms	1613:1617	terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains	1613:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	9	25	from	fluorescence	1656:1667	arg1	strains					1702:1708	control versus NP-treated MDR strains	1672:1708	control versus NP-treated MDR strains	1672:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	8	26	theme	time-kill	1479:1487	arg1	analysis					1495:1502	time-kill curve analysis	1479:1502	time-kill curve analysis	1479:1502	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	27	theme	≤0.5	1345:1348	arg1	values					1350:1355	≤0.5 values	1345:1355	≤0.5 values	1345:1355	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	2	28	theme	community	417:425	arg1	microbes					427:434	community microbes	417:434	community microbes	417:434	Unfortunately, ABR is no longer confined to nosocomial environments and is frequently reported from community microbes as well.					
33989619	0	29	theme	Embelin-loaded	0:13	arg1	nanoparticles					29:41	Embelin-loaded chitosan gold nanoparticles	0:41	Embelin-loaded chitosan gold nanoparticles	0:41	Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.					
33989619	10	30	dep	PA-r	1867:1870	arg1	OprM					1888:1891	OprM	1888:1891	OprM	1888:1891	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	30	dep	PA-r	1867:1870	arg1	MexB					1879:1882	MexB	1879:1882	MexB	1879:1882	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	30	dep	PA-r	1867:1870	arg1	MexA					1873:1876	MexA	1873:1876	MexA	1873:1876	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	3	31	theme	potent	479:484	arg1	pool					498:501	potent antibiotics pool	479:501	potent antibiotics pool	479:501	The ABR is resulting in shrinking potent antibiotics pool and thus necessitating novel and alternative therapies and therapeutics.					
33989619	0	32	theme	gold	24:27	arg1	nanoparticles					29:41	Embelin-loaded chitosan gold nanoparticles	0:41	Embelin-loaded chitosan gold nanoparticles	0:41	Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.					
33989619	6	33	theme	Synthesized	909:919	arg1	NPs					1009:1011	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs	909:1011	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs	909:1011	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	9	34	theme	action	1538:1543	arg1	mode					1530:1533	the mode	1526:1533	the mode of action	1526:1543	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	7	35	theme	CIP	1209:1211	arg1	concentration					1186:1198	the minimal inhibitory concentration	1163:1198	the minimal inhibitory concentration (MIC) of CIP	1163:1211	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	7	35	theme	CIP	1209:1211	arg1	MIC					1201:1203	MIC	1201:1203	MIC	1201:1203	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	6	36	attach	isolated	935:942	arg1	tsjeriam-cottam					957:971	Embelia tsjeriam-cottam	949:971	Embelia tsjeriam-cottam	949:971	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	6	36	attach	isolated	935:942	arg2	Emb					930:932	Emb	930:932	Emb	930:932	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	5	37	theme	ABR	829:831	arg1	profiling					833:841	ABR profiling	829:841	ABR profiling of these strains	829:858	ABR profiling of these strains characterized them as multidrug resistant (MDR).					
33989619	9	38	theme	efflux	1546:1551	arg1	pump					1553:1556	efflux pump	1546:1556	efflux pump inhibitory effects of Emb-Chi-Au NPs	1546:1593	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	7	39	theme	inhibitory	1175:1184	arg1	concentration					1186:1198	the minimal inhibitory concentration	1163:1198	the minimal inhibitory concentration (MIC) of CIP	1163:1211	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	7	39	theme	inhibitory	1175:1184	arg1	MIC					1201:1203	MIC	1201:1203	MIC	1201:1203	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	4	40	dep	phytomolecule-loaded	662:681	arg1	polysaccharide-stabilized					684:708	polysaccharide-stabilized	684:708	polysaccharide-stabilized	684:708	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	10	41	theme	probable	1945:1952	arg1	formation					1959:1967	the probable bond formation	1941:1967	the probable bond formation between Emb and respective amino acid residues	1941:2014	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	6	42	dep	embelin	921:927	arg1	Emb					930:932	Emb	930:932	Emb	930:932	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	3	43	theme	alternative	536:546	arg1	therapies					548:556	novel and alternative therapies	526:556	novel and alternative therapies	526:556	The ABR is resulting in shrinking potent antibiotics pool and thus necessitating novel and alternative therapies and therapeutics.					
33989619	1	44	theme	bacterial	257:265	arg1	populations					267:277	bacterial populations	257:277	bacterial populations	257:277	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	9	45	theme	NPs	1591:1593	arg1	effects					1569:1575	efflux pump inhibitory effects	1546:1575	efflux pump inhibitory effects of Emb-Chi-Au NPs	1546:1593	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	7	46	theme	EC	1257:1258	arg1	EC-r					1261:1264	EC-r	1261:1264	EC-r	1261:1264	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	7	46	theme	EC	1257:1258	arg1	strains					1267:1273	MDR PA (PA-r) and EC (EC-r) strains	1239:1273	strains	1267:1273	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	10	47	theme	respective	1985:1994	arg1	residues					2007:2014	respective amino acid residues	1985:2014	respective amino acid residues	1985:2014	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	3	48	theme	novel	526:530	arg1	therapies					548:556	novel and alternative therapies	526:556	novel and alternative therapies	526:556	The ABR is resulting in shrinking potent antibiotics pool and thus necessitating novel and alternative therapies and therapeutics.					
33989619	6	49	theme	time-kill	1121:1129	arg1	analysis					1137:1144	time-kill curve analysis	1121:1144	time-kill curve analysis	1121:1144	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	10	50	dep	Emb	1803:1805	arg1	proteins					1855:1862	related proteins	1847:1862	related proteins	1847:1862	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	51	theme	pump	1842:1845	arg1	Emb					1803:1805	Emb	1803:1805	Emb	1803:1805	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	51	theme	pump	1842:1845	arg1	pump					1842:1845	the efflux pump	1831:1845	the efflux pump	1831:1845	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	51	theme	pump	1842:1845	arg1	sites					1822:1826	the active sites	1811:1826	the active sites of the efflux pump	1811:1845	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	52	theme	Molecular	1711:1719	arg1	simulations					1745:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	1	53	theme	serious	284:290	arg1	upsurge					191:197	A global upsurge	182:197	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations	182:277	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	1	53	theme	serious	284:290	arg1	threat					292:297	a serious threat	282:297	a serious threat for human health	282:314	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	4	54	theme	synergistic	622:632	arg1	potential					634:642	the synergistic potential	618:642	the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters	618:826	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	10	55	theme	acid	2002:2005	arg1	residues					2007:2014	respective amino acid residues	1985:2014	respective amino acid residues	1985:2014	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	56	dep	predict	1770:1776	arg1	revealed					1932:1939	revealed	1932:1939	revealed the probable bond formation between Emb and respective amino acid residues	1932:2014	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	7	57	theme	MDR	1239:1241	arg1	PA-r					1247:1250	PA-r	1247:1250	PA-r	1247:1250	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	7	57	theme	MDR	1239:1241	arg1	PA					1243:1244	MDR PA (PA-r) and EC (EC-r) strains	1239:1273	PA	1243:1244	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	9	58	from	increase	1626:1633	arg1	fluorescence					1656:1667	the EtBr mediated fluorescence	1638:1667	the EtBr mediated fluorescence in control versus NP-treated MDR strains	1638:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	9	59	theme	inhibitory	1558:1567	arg1	effects					1569:1575	efflux pump inhibitory effects	1546:1575	efflux pump inhibitory effects of Emb-Chi-Au NPs	1546:1593	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	10	60	theme	based	1729:1733	arg1	simulations					1745:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	1	61	theme	antibiotic	226:235	arg1	resistance					237:246	antibiotic resistance	226:246	antibiotic resistance (ABR)	226:252	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	1	61	theme	antibiotic	226:235	arg1	ABR					249:251	ABR	249:251	ABR	249:251	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	6	62	dep	-loaded	973:979	arg1	chitosan-gold					982:994	chitosan-gold	982:994	chitosan-gold	982:994	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	4	63	theme	phytomolecule-loaded	662:681	arg1	a					647:647	a	647:647	a	647:647	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	4	63	theme	phytomolecule-loaded	662:681	arg1	NPs					734:736	NPs	734:736	NPs	734:736	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	4	63	theme	phytomolecule-loaded	662:681	arg1	nanoparticles					719:731	phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles	662:731	phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs)	662:737	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	6	64	dep	chitosan-gold	982:994	arg1	Emb-Chi-Au					997:1006	Emb-Chi-Au	997:1006	Emb-Chi-Au	997:1006	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	6	65	theme	-loaded	973:979	arg1	NPs					1009:1011	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs	909:1011	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs	909:1011	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	9	66	theme	control	1672:1678	arg1	strains					1702:1708	control versus NP-treated MDR strains	1672:1708	control versus NP-treated MDR strains	1672:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	6	67	with	activity	1059:1066	arg1	CIP					1088:1090	CIP	1088:1090	CIP	1088:1090	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	6	67	with	activity	1059:1066	arg1	ciprofloxacin					1073:1085	ciprofloxacin	1073:1085	ciprofloxacin (CIP)	1073:1091	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	9	68	theme	NP-treated	1687:1696	arg1	strains					1702:1708	control versus NP-treated MDR strains	1672:1708	control versus NP-treated MDR strains	1672:1708	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	10	69	dep	in	1735:1736	arg1	silico					1738:1743	silico	1738:1743	silico	1738:1743	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	8	70	dep	NPs	1402:1404	arg1	the					1387:1389	the	1387:1389	the	1387:1389	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	6	71	theme	synergistic	1047:1057	arg1	activity					1059:1066	their potential synergistic activity	1031:1066	their potential synergistic activity with ciprofloxacin (CIP)	1031:1091	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	10	72	dep	EC-r	1898:1901	arg1	TolC					1919:1922	TolC	1919:1922	TolC	1919:1922	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	72	dep	EC-r	1898:1901	arg1	AcrB					1910:1913	AcrB	1910:1913	AcrB	1910:1913	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	72	dep	EC-r	1898:1901	arg1	AcrA					1904:1907	AcrA	1904:1907	AcrA	1904:1907	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	8	73	theme	inhibitory	1301:1310	arg1	concentration					1312:1324	Fractional inhibitory concentration	1290:1324	Fractional inhibitory concentration (FIC) indices with ≤0.5 values	1290:1355	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	73	theme	inhibitory	1301:1310	arg1	FIC					1327:1329	FIC	1327:1329	FIC	1327:1329	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	10	74	used	used	1762:1765	arg2	simulations					1745:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations	1711:1755	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	0	75	from	pumps	108:112	arg1	coli					176:179	Escherichia coli	164:179	Escherichia coli	164:179	Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.					
33989619	0	75	from	pumps	108:112	arg1	aeruginosa					149:158	Pseudomonas aeruginosa	137:158	Pseudomonas aeruginosa	137:158	Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.					
33989619	8	76	theme	curve	1489:1493	arg1	analysis					1495:1502	time-kill curve analysis	1479:1502	time-kill curve analysis	1479:1502	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	77	from	MICs	1448:1451	arg1	EC-r					1470:1473	EC-r	1470:1473	EC-r	1470:1473	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	8	77	from	MICs	1448:1451	arg1	PA-r					1461:1464	PA-r	1461:1464	PA-r	1461:1464	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	4	78	theme	river	815:819	arg1	waters					821:826	river waters	815:826	river waters	815:826	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	0	79	theme	chitosan	15:22	arg1	nanoparticles					29:41	Embelin-loaded chitosan gold nanoparticles	0:41	Embelin-loaded chitosan gold nanoparticles	0:41	Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.					
33989619	6	80	theme	checker-board	1097:1109	arg1	assay					1111:1115	checker-board assay	1097:1115	checker-board assay	1097:1115	Synthesized embelin (Emb, isolated from Embelia tsjeriam-cottam)-loaded, chitosan-gold (Emb-Chi-Au) NPs were assessed for their potential synergistic activity with ciprofloxacin (CIP) via checker-board assay and time-kill curve analysis.					
33989619	1	81	theme	human	303:307	arg1	health					309:314	human health	303:314	human health	303:314	A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health.					
33989619	10	82	theme	active	1815:1820	arg1	pump					1842:1845	the efflux pump	1831:1845	the efflux pump	1831:1845	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	10	82	theme	active	1815:1820	arg1	sites					1822:1826	the active sites	1811:1826	the active sites of the efflux pump	1811:1845	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	3	83	theme	antibiotics	486:496	arg1	pool					498:501	potent antibiotics pool	479:501	potent antibiotics pool	479:501	The ABR is resulting in shrinking potent antibiotics pool and thus necessitating novel and alternative therapies and therapeutics.					
33989619	7	84	theme	minimal	1167:1173	arg1	concentration					1186:1198	the minimal inhibitory concentration	1163:1198	the minimal inhibitory concentration (MIC) of CIP	1163:1211	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	7	84	theme	minimal	1167:1173	arg1	MIC					1201:1203	MIC	1201:1203	MIC	1201:1203	The NPs reduced the minimal inhibitory concentration (MIC) of CIP by 16- and 4-fold against MDR PA (PA-r) and EC (EC-r) strains, respectively.					
33989619	9	85	theme	pump	1553:1556	arg1	effects					1569:1575	efflux pump inhibitory effects	1546:1575	efflux pump inhibitory effects of Emb-Chi-Au NPs	1546:1593	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
33989619	4	86	theme	Current	576:582	arg1	investigation					584:596	Current investigation	576:596	Current investigation	576:596	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	10	87	theme	efflux	1835:1840	arg1	pump					1842:1845	the efflux pump	1831:1845	the efflux pump	1831:1845	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	8	88	theme	Emb-Chi-Au	1391:1400	arg1	NPs					1402:1404	Emb-Chi-Au NPs	1391:1404	Emb-Chi-Au NPs	1391:1404	Fractional inhibitory concentration (FIC) indices with ≤0.5 values confirmed the synergy between the Emb-Chi-Au NPs and CIP, which was further confirmed at ½ MICs in both PA-r and EC-r via time-kill curve analysis.					
33989619	10	89	theme	bond	1954:1957	arg1	formation					1959:1967	the probable bond formation	1941:1967	the probable bond formation between Emb and respective amino acid residues	1941:2014	Molecular docking based in silico simulations were used to predict the interactions between Emb and the active sites of the efflux pump related proteins in PA-r (MexA, MexB and OprM) and EC-r (AcrA, AcrB and TolC), which revealed the probable bond formation between Emb and respective amino acid residues.					
33989619	4	90	theme	a	647:647	arg1	potential					634:642	the synergistic potential	618:642	the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters	618:826	Current investigation was aimed to assess the synergistic potential of a synthesized, phytomolecule-loaded, polysaccharide-stabilized metallic nanoparticles (NPs) against Pseudomonas aeruginosa (PA) and Escherichia coli (EC) isolated from river waters.					
33989619	9	91	theme	Emb-Chi-Au	1580:1589	arg1	NPs					1591:1593	Emb-Chi-Au NPs	1580:1593	Emb-Chi-Au NPs	1580:1593	In order to decipher the mode of action, efflux pump inhibitory effects of Emb-Chi-Au NPs were evaluated in terms of the increase in the EtBr mediated fluorescence in control versus NP-treated MDR strains.					
32893709	11	0	theme	butyric	1289:1295	arg1	acids					1332:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids.					
32893709	4	1	theme	intervention	480:491	arg1	effect					461:466	the effect	457:466	the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites	457:594	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	0	2	with	interaction	33:43	arg1	microbiota					75:84	the gut microbiota	67:84	the gut microbiota	67:84	Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota.					
32893709	11	3	theme	bacterial	1248:1256	arg1	metabolites					1258:1268	bacterial metabolites	1248:1268	bacterial metabolites	1248:1268	In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids.					
32893709	10	4	theme	microbiota	1119:1128	arg1	composition					1130:1140	the gut microbiota composition	1111:1140	the gut microbiota composition	1111:1140	CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation.					
32893709	8	5	theme	gut	940:942	arg1	microbiota					944:953	The gut microbiota	936:953	The gut microbiota (primary outcome)	936:971	The gut microbiota (primary outcome) was analyzed by Illumina sequencing.					
32893709	8	5	theme	gut	940:942	arg1	outcome					964:970	primary outcome	956:970	primary outcome	956:970	The gut microbiota (primary outcome) was analyzed by Illumina sequencing.					
32893709	6	6	theme	survey	766:771	arg1	analog					705:710	Food diary, visual analog and Bristol stool form scales	686:740	analog	705:710	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	6	6	theme	survey	766:771	arg1	quality					749:755	a "quality	746:755	a "quality of life" survey	746:771	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	6	6	theme	survey	766:771	arg1	scales					735:740	Food diary, visual analog and Bristol stool form scales	686:740	scales	735:740	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	7	7	theme	bile	830:833	arg1	BA					842:843	BA	842:843	BA	842:843	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	7	7	theme	bile	830:833	arg1	acids					835:839	bile acids	830:839	bile acids (BA)	830:844	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	4	8	theme	bacterial	574:582	arg1	metabolites					584:594	bacterial metabolites	574:594	bacterial metabolites	574:594	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	11	9	theme	vaccenic	1323:1330	arg1	acids					1332:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids.					
32893709	4	10	theme	CG	498:499	arg1	supplementation					501:515	CG supplementation	498:515	CG supplementation	498:515	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	8	11	theme	primary	956:962	arg1	microbiota					944:953	The gut microbiota	936:953	The gut microbiota (primary outcome)	936:971	The gut microbiota (primary outcome) was analyzed by Illumina sequencing.					
32893709	8	11	theme	primary	956:962	arg1	outcome					964:970	primary outcome	956:970	primary outcome	956:970	The gut microbiota (primary outcome) was analyzed by Illumina sequencing.					
32893709	6	12	theme	"	764:764	arg1	survey					766:771	life" survey	760:771	life" survey	760:771	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	4	13	from	intervention	480:491	arg1	individuals					528:538	healthy individuals	520:538	healthy individuals	520:538	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	6	14	theme	life	760:763	arg1	survey					766:771	life" survey	760:771	life" survey	760:771	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	6	15	theme	diary	691:695	arg1	analog					705:710	Food diary, visual analog and Bristol stool form scales	686:740	analog	705:710	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	4	16	with	intervention	480:491	arg1	supplementation					501:515	CG supplementation	498:515	CG supplementation	498:515	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	11	17	theme	caproic	1311:1317	arg1	acids					1332:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids.					
32893709	3	18	from	effect	376:381	arg1	microbiota					390:399	gut microbiota	386:399	gut microbiota	386:399	In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans.					
32893709	10	19	theme	strongest	1200:1208	arg1	regulation					1210:1219	the strongest regulation	1196:1219	the strongest regulation	1196:1219	CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation.					
32893709	5	20	theme	healthy	613:619	arg1	n = 15					633:638	n = 15	633:638	n = 15	633:638	CG was given to healthy volunteers (n = 15) for three weeks as a supplement (4.5 g/day).					
32893709	5	20	theme	healthy	613:619	arg1	volunteers					621:630	healthy volunteers	613:630	healthy volunteers (n = 15)	613:639	CG was given to healthy volunteers (n = 15) for three weeks as a supplement (4.5 g/day).					
32893709	6	21	theme	Food	686:689	arg1	analog					705:710	Food diary, visual analog and Bristol stool form scales	686:740	analog	705:710	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	2	22	theme	Current	150:156	arg1	data					158:161	Current data	150:161	Current data	150:161	Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects.					
32893709	13	23	theme	fiber	1581:1585	arg1	-CG-					1587:1590	an insoluble dietary fiber -CG-	1560:1590	an insoluble dietary fiber -CG-	1560:1590	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	0	24	theme	Metabolite	0:9	arg1	profiling					11:19	Metabolite profiling	0:19	Metabolite profiling	0:19	Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota.					
32893709	7	25	theme	gut	794:796	arg1	metabolites					817:827	gut microbiota-derived metabolites	794:827	gut microbiota-derived metabolites	794:827	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	12	26	theme	major	1342:1346	arg1	changes					1348:1354	No major changes	1339:1354	No major changes	1339:1354	No major changes were observed for the fecal BA profile following CG intervention.					
32893709	11	27	theme	iso-valeric	1298:1308	arg1	acids					1332:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	butyric, iso-valeric, caproic and vaccenic acids	1289:1336	In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids.					
32893709	1	28	theme	Dietary	87:93	arg1	fibers					95:100	Dietary fibers	87:100	Dietary fibers	87:100	Dietary fibers are considered beneficial nutrients for health.					
32893709	7	29	link	microbiota-derived	798:815	arg1	metabolites					817:827	gut microbiota-derived metabolites	794:827	gut microbiota-derived metabolites	794:827	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	3	30	theme	gut	386:388	arg1	microbiota					390:399	gut microbiota	386:399	gut microbiota	386:399	In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans.					
32893709	4	31	theme	microbiota	547:556	arg1	composition					558:568	gut microbiota composition	543:568	gut microbiota composition	543:568	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	4	32	from	effect	461:466	arg1	individuals					528:538	healthy individuals	520:538	healthy individuals	520:538	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	4	32	from	effect	461:466	arg1	composition					558:568	gut microbiota composition	543:568	gut microbiota composition	543:568	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	4	32	from	effect	461:466	arg1	metabolites					584:594	bacterial metabolites	574:594	bacterial metabolites	574:594	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	7	33	theme	short-chain	857:867	arg1	acids					875:879	short-chain fatty acids	857:879	short-chain fatty acids (LCFA, SCFA)	857:892	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	7	33	theme	short-chain	857:867	arg1	LCFA					882:885	LCFA	882:885	LCFA	882:885	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	13	34	theme	gut	1600:1602	arg1	microbiota					1604:1613	the gut microbiota	1596:1613	the gut microbiota	1596:1613	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	7	35	theme	microbiota-derived	798:815	arg1	metabolites					817:827	gut microbiota-derived metabolites	794:827	gut microbiota-derived metabolites	794:827	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	4	36	theme	gut	543:545	arg1	composition					558:568	gut microbiota composition	543:568	gut microbiota composition	543:568	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	8	37	theme	Illumina	989:996	arg1	sequencing					998:1007	Illumina sequencing	989:1007	Illumina sequencing	989:1007	The gut microbiota (primary outcome) was analyzed by Illumina sequencing.					
32893709	7	38	theme	fatty	869:873	arg1	acids					875:879	short-chain fatty acids	857:879	short-chain fatty acids (LCFA, SCFA)	857:892	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	7	38	theme	fatty	869:873	arg1	LCFA					882:885	LCFA	882:885	LCFA	882:885	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	13	39	theme	gut	1486:1488	arg1	metabolites					1509:1519	gut microbiota-derived metabolites	1486:1519	gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota	1486:1613	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	9	40	theme	3-week	1012:1017	arg1	supplementation					1019:1033	A 3-week supplementation	1010:1033	A 3-week supplementation with CG	1010:1041	A 3-week supplementation with CG is well tolerated in healthy humans.					
32893709	7	41	theme	stool	921:925	arg1	samples					927:933	stool samples	921:933	stool samples	921:933	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	9	42	with	supplementation	1019:1033	arg1	CG					1040:1041	CG	1040:1041	CG	1040:1041	A 3-week supplementation with CG is well tolerated in healthy humans.					
32893709	13	43	theme	microbiota-derived	1490:1507	arg1	metabolites					1509:1519	gut microbiota-derived metabolites	1486:1519	gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota	1486:1613	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	10	44	from	changes	1100:1106	arg1	composition					1130:1140	the gut microbiota composition	1111:1140	the gut microbiota composition	1111:1140	CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation.					
32893709	12	45	theme	CG	1405:1406	arg1	intervention					1408:1419	CG intervention	1405:1419	CG intervention	1405:1419	No major changes were observed for the fecal BA profile following CG intervention.					
32893709	2	46	theme	physiological	247:259	arg1	effects					261:267	their physiological effects	241:267	their physiological effects	241:267	Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects.					
32893709	4	47	from	individuals	528:538	arg1	effect					461:466	the effect	457:466	the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites	457:594	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	4	48	theme	healthy	520:526	arg1	individuals					528:538	healthy individuals	520:538	healthy individuals	520:538	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	10	49	theme	gut	1115:1117	arg1	composition					1130:1140	the gut microbiota composition	1111:1140	the gut microbiota composition	1111:1140	CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation.					
32893709	13	50	theme	new	1451:1453	arg1	genera					1475:1480	new potential bacterial genera	1451:1480	new potential bacterial genera	1451:1480	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	10	51	dep	Dorea	1161:1165	arg1	genera					1181:1186	genera	1181:1186	genera	1181:1186	CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation.					
32893709	13	52	theme	potential	1455:1463	arg1	genera					1475:1480	new potential bacterial genera	1451:1480	new potential bacterial genera	1451:1480	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	4	53	theme	3-week	473:478	arg1	intervention					480:491	a 3-week intervention	471:491	a 3-week intervention with CG supplementation in healthy individuals	471:538	This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites.					
32893709	1	54	theme	beneficial	117:126	arg1	nutrients					128:136	beneficial nutrients	117:136	beneficial nutrients	117:136	Dietary fibers are considered beneficial nutrients for health.					
32893709	0	55	theme	chitin-glucan	48:60	arg1	interaction					33:43	the interaction	29:43	the interaction of chitin-glucan with the gut microbiota	29:84	Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota.					
32893709	3	56	theme	metabolic	315:323	arg1	disorders					325:333	metabolic disorders	315:333	metabolic disorders associated with obesity in mice	315:365	In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans.					
32893709	12	57	theme	BA	1384:1385	arg1	profile					1387:1393	the fecal BA profile	1374:1393	the fecal BA profile following CG intervention	1374:1419	No major changes were observed for the fecal BA profile following CG intervention.					
32893709	13	58	theme	bacterial	1465:1473	arg1	genera					1475:1480	new potential bacterial genera	1451:1480	new potential bacterial genera	1451:1480	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	6	59	theme	stool	724:728	arg1	scales					735:740	Food diary, visual analog and Bristol stool form scales	686:740	scales	735:740	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	7	60	dep	LCFA	882:885	arg1	SCFA					888:891	SCFA	888:891	SCFA	888:891	Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples.					
32893709	13	61	theme	insoluble	1563:1571	arg1	-CG-					1587:1590	an insoluble dietary fiber -CG-	1560:1590	an insoluble dietary fiber -CG-	1560:1590	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	12	62	theme	fecal	1378:1382	arg1	profile					1387:1393	the fecal BA profile	1374:1393	the fecal BA profile following CG intervention	1374:1419	No major changes were observed for the fecal BA profile following CG intervention.					
32893709	6	63	theme	Bristol	716:722	arg1	scales					735:740	Food diary, visual analog and Bristol stool form scales	686:740	scales	735:740	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	6	64	theme	form	730:733	arg1	scales					735:740	Food diary, visual analog and Bristol stool form scales	686:740	scales	735:740	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
32893709	13	65	theme	dietary	1573:1579	arg1	-CG-					1587:1590	an insoluble dietary fiber -CG-	1560:1590	an insoluble dietary fiber -CG-	1560:1590	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	2	66	theme	gut	203:205	arg1	microbiota					207:216	the gut microbiota	199:216	the gut microbiota	199:216	Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects.					
32893709	2	67	with	interaction	182:192	arg1	microbiota					207:216	the gut microbiota	199:216	the gut microbiota	199:216	Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects.					
32893709	10	68	theme	specific	1091:1098	arg1	changes					1100:1106	specific changes	1091:1106	specific changes in the gut microbiota composition	1091:1140	CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation.					
32893709	0	69	theme	gut	71:73	arg1	microbiota					75:84	the gut microbiota	67:84	the gut microbiota	67:84	Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota.					
32893709	3	70	from	obesity	351:357	arg1	mice					362:365	mice	362:365	mice	362:365	In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans.					
32893709	9	71	theme	healthy	1064:1070	arg1	humans					1072:1077	healthy humans	1064:1077	healthy humans	1064:1077	A 3-week supplementation with CG is well tolerated in healthy humans.					
32893709	13	72	link	microbiota-derived	1490:1507	arg1	metabolites					1509:1519	gut microbiota-derived metabolites	1486:1519	gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota	1486:1613	In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.					
32893709	6	73	theme	visual	698:703	arg1	analog					705:710	Food diary, visual analog and Bristol stool form scales	686:740	analog	705:710	Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed.					
33766331	8	0	theme	large	1217:1221	arg1	number					1223:1228	a large number	1215:1228	a large number of membrane proteins responsible for substance transport and biochemical reactions	1215:1311	Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated.					
33766331	5	1	theme	electron	884:891	arg1	TEM					905:907	TEM	905:907	TEM	905:907	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	1	theme	electron	884:891	arg1	microscopy					893:902	transmission electron microscopy	871:902	transmission electron microscopy (TEM)	871:908	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	1	2	theme	plant	237:241	arg1	diseases					250:257	plant fungal diseases	237:257	plant fungal diseases	237:257	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	5	3	theme	apparent	760:767	arg1	changes					783:789	apparent morphological changes	760:789	apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs)	760:866	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	7	4	theme	fatty	1104:1108	arg1	UFAs					1117:1120	UFAs	1117:1120	UFAs	1117:1120	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	7	4	theme	fatty	1104:1108	arg1	acids					1110:1114	Many unsaturated fatty acids	1087:1114	Many unsaturated fatty acids (UFAs)	1087:1121	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	6	5	from	interest	1038:1045	arg1	properties					1075:1084	membrane physicochemical properties	1050:1084	membrane physicochemical properties	1050:1084	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	2	6	theme	structure	327:335	arg1	model					300:304	a negative remodeling model	278:304	a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	278:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	1	7	theme	fungal	243:248	arg1	diseases					250:257	plant fungal diseases	237:257	plant fungal diseases	237:257	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	7	8	theme	cell	1170:1173	arg1	membrane					1175:1182	the cell membrane	1166:1182	the cell membrane	1166:1182	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	5	9	from	changes	783:789	arg1	GUVs					862:865	GUVs	862:865	GUVs	862:865	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	9	from	changes	783:789	arg1	membranes					798:806	the membranes	794:806	the membranes of P. capsici Leonian	794:828	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	9	from	changes	783:789	arg1	vesicles					852:859	giant unilamellar vesicles	834:859	giant unilamellar vesicles (GUVs)	834:866	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	7	10	theme	unsaturated	1092:1102	arg1	UFAs					1117:1120	UFAs	1117:1120	UFAs	1117:1120	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	7	10	theme	unsaturated	1092:1102	arg1	acids					1110:1114	Many unsaturated fatty acids	1087:1114	Many unsaturated fatty acids (UFAs)	1087:1121	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	10	11	theme	molecular	1600:1608	arg1	target					1610:1615	a potential molecular target	1588:1615	a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry	1588:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	10	11	theme	molecular	1600:1608	arg1	aquaporin					1558:1566	aquaporin 10	1558:1569	aquaporin 10	1558:1569	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	10	12	theme	structure	1697:1705	arg1	analysis					1676:1683	composition analysis	1664:1683	composition analysis of membrane structure and immunohistochemistry	1664:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	6	13	theme	membrane	961:968	arg1	analysis					940:947	component analysis	930:947	component analysis of the cell membrane	930:968	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	7	14	theme	membrane	1175:1182	arg1	synthesis					1153:1161	UFA synthesis	1149:1161	UFA synthesis of the cell membrane	1149:1182	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	6	15	theme	cell	956:959	arg1	membrane					961:968	the cell membrane	952:968	the cell membrane	952:968	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	5	16	theme	giant	834:838	arg1	GUVs					862:865	GUVs	862:865	GUVs	862:865	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	16	theme	giant	834:838	arg1	vesicles					852:859	giant unilamellar vesicles	834:859	giant unilamellar vesicles (GUVs)	834:866	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	0	17	theme	membrane	104:111	arg1	integrity					113:121	the cell membrane integrity	95:121	the cell membrane integrity of Phytophthora capsici Leonian	95:153	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	4	18	theme	isothiocyanate	697:710	arg1	-fluorescence					718:730	fluorescein isothiocyanate (FITC)-fluorescence	685:730	fluorescein isothiocyanate (FITC)-fluorescence	685:730	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	6	19	theme	membrane	1050:1057	arg1	properties					1075:1084	membrane physicochemical properties	1050:1084	membrane physicochemical properties	1050:1084	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	10	20	theme	membrane	1688:1695	arg1	structure					1697:1705	membrane structure	1688:1705	membrane structure	1688:1705	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	2	21	theme	Cu	391:392	arg1	O-CSLn-Cu					408:416	O-CSLn-Cu	408:416	O-CSLn-Cu	408:416	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	2	21	theme	Cu	391:392	arg1	complex					399:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex	348:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	348:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	0	22	theme	cell	99:102	arg1	integrity					113:121	the cell membrane integrity	95:121	the cell membrane integrity of Phytophthora capsici Leonian	95:153	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	10	23	theme	immunohistochemistry	1711:1730	arg1	analysis					1676:1683	composition analysis	1664:1683	composition analysis of membrane structure and immunohistochemistry	1664:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	7	24	theme	Many	1087:1090	arg1	UFAs					1117:1120	UFAs	1117:1120	UFAs	1117:1120	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	7	24	theme	Many	1087:1090	arg1	acids					1110:1114	Many unsaturated fatty acids	1087:1114	Many unsaturated fatty acids (UFAs)	1087:1121	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	4	25	theme	fungal	593:598	arg1	membrane					605:612	the fungal cell membrane	589:612	the fungal cell membrane	589:612	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	1	26	theme	cell	170:173	arg1	membrane					175:182	the cell membrane	166:182	the cell membrane	166:182	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	0	27	theme	O-carboxymethyl	14:28	arg1	complex					46:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	4	28	theme	FITC-labeled	547:558	arg1	O-CSLn-Cu					560:568	FITC-labeled O-CSLn-Cu	547:568	FITC-labeled O-CSLn-Cu	547:568	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	4	29	theme	laser	639:643	arg1	CLSM					666:669	CLSM	666:669	CLSM	666:669	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	4	29	theme	laser	639:643	arg1	microscopy					654:663	confocal laser scanning microscopy	630:663	confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence	630:730	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	7	30	theme	UFA	1149:1151	arg1	synthesis					1153:1161	UFA synthesis	1149:1161	UFA synthesis of the cell membrane	1149:1182	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	2	31	theme	O-carboxymethyl	366:380	arg1	O-CSLn-Cu					408:416	O-CSLn-Cu	408:416	O-CSLn-Cu	408:416	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	2	31	theme	O-carboxymethyl	366:380	arg1	complex					399:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex	348:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	348:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	0	32	theme	C-coordinated	0:12	arg1	complex					46:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	7	33	theme	key	1127:1129	arg1	enzymes					1131:1137	key enzymes	1127:1137	key enzymes promoting UFA synthesis of the cell membrane	1127:1182	Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated.					
33766331	2	34	theme	remodeling	289:298	arg1	model					300:304	a negative remodeling model	278:304	a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	278:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	2	35	theme	C-coordinated	352:364	arg1	O-CSLn-Cu					408:416	O-CSLn-Cu	408:416	O-CSLn-Cu	408:416	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	2	35	theme	C-coordinated	352:364	arg1	complex					399:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex	348:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	348:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	0	36	theme	Cu	39:40	arg1	complex					46:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	9	37	theme	peroxidation	1501:1512	arg1	leakage					1423:1429	significant leakage	1411:1429	significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation	1411:1512	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	9	38	theme	soluble	1462:1468	arg1	proteins					1470:1477	proteins	1470:1477	proteins	1470:1477	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	2	39	theme	negative	280:287	arg1	model					300:304	a negative remodeling model	278:304	a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	278:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	8	40	theme	biochemical	1291:1301	arg1	reactions					1303:1311	biochemical reactions	1291:1311	biochemical reactions	1291:1311	Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated.					
33766331	5	41	theme	Leonian	822:828	arg1	GUVs					862:865	GUVs	862:865	GUVs	862:865	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	41	theme	Leonian	822:828	arg1	vesicles					852:859	giant unilamellar vesicles	834:859	giant unilamellar vesicles (GUVs)	834:866	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	41	theme	Leonian	822:828	arg1	membranes					798:806	the membranes	794:806	the membranes of P. capsici Leonian	794:828	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	0	42	theme	chitosan	30:37	arg1	complex					46:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex	0:52	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	6	43	theme	physicochemical	1059:1073	arg1	properties					1075:1084	membrane physicochemical properties	1050:1084	membrane physicochemical properties	1050:1084	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	1	44	theme	effective	190:198	arg1	Damage					156:161	Damage	156:161	Damage to the cell membrane	156:182	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	1	44	theme	effective	190:198	arg1	method					200:205	an effective method	187:205	an effective method to prevent drug resistance in plant fungal diseases	187:257	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	5	45	theme	morphological	769:781	arg1	changes					783:789	apparent morphological changes	760:789	apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs)	760:866	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	4	46	theme	fluorescein	685:695	arg1	isothiocyanate					697:710	fluorescein isothiocyanate	685:710	fluorescein isothiocyanate (FITC)-fluorescence	685:730	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	4	46	theme	fluorescein	685:695	arg1	FITC					713:716	FITC	713:716	FITC	713:716	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	0	47	theme	capsici	139:145	arg1	Leonian					147:153	Phytophthora capsici Leonian	126:153	Phytophthora capsici Leonian	126:153	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	4	48	theme	confocal	630:637	arg1	CLSM					666:669	CLSM	666:669	CLSM	666:669	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	4	48	theme	confocal	630:637	arg1	microscopy					654:663	confocal laser scanning microscopy	630:663	confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence	630:730	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	6	49	theme	component	930:938	arg1	analysis					940:947	component analysis	930:947	component analysis of the cell membrane	930:968	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	6	50	theme	particular	1027:1036	arg1	interest					1038:1045	a particular interest	1025:1045	a particular interest in membrane physicochemical properties	1025:1084	Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by O-CSLn-Cu with a particular interest in membrane physicochemical properties.					
33766331	3	51	theme	FITC-labeled	420:431	arg1	O-CSLn-Cu					433:441	FITC-labeled O-CSLn-Cu	420:441	FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu)	420:458	FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR.					
33766331	3	51	theme	FITC-labeled	420:431	arg1	FITC-O-CSLn-Cu					444:457	FITC-O-CSLn-Cu	444:457	FITC-O-CSLn-Cu	444:457	FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR.					
33766331	4	52	theme	scanning	645:652	arg1	CLSM					666:669	CLSM	666:669	CLSM	666:669	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	4	52	theme	scanning	645:652	arg1	microscopy					654:663	confocal laser scanning microscopy	630:663	confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence	630:730	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	0	53	theme	Phytophthora	126:137	arg1	Leonian					147:153	Phytophthora capsici Leonian	126:153	Phytophthora capsici Leonian	126:153	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	8	54	theme	responsible	1251:1261	arg1	proteins					1242:1249	membrane proteins	1233:1249	membrane proteins responsible for substance transport and biochemical reactions	1233:1311	Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated.					
33766331	10	55	theme	sensitive	1617:1625	arg1	target					1610:1615	a potential molecular target	1588:1615	a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry	1588:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	10	55	theme	sensitive	1617:1625	arg1	aquaporin					1558:1566	aquaporin 10	1558:1569	aquaporin 10	1558:1569	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	5	56	theme	O-CSLn-Cu	733:741	arg1	treatment					743:751	O-CSLn-Cu treatment	733:751	O-CSLn-Cu treatment	733:751	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	8	57	theme	substance	1267:1275	arg1	transport					1277:1285	substance transport	1267:1285	substance transport	1267:1285	Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated.					
33766331	9	58	theme	proteins	1470:1477	arg1	leakage					1423:1429	significant leakage	1411:1429	significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation	1411:1512	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	1	59	from	resistance	223:232	arg1	diseases					250:257	plant fungal diseases	237:257	plant fungal diseases	237:257	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	9	60	theme	lipid	1495:1499	arg1	peroxidation					1501:1512	lipid peroxidation	1495:1512	lipid peroxidation	1495:1512	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	2	61	theme	membrane	318:325	arg1	structure					327:335	the cell membrane structure	309:335	the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	309:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	9	62	theme	plasma	1377:1382	arg1	permeability					1393:1404	plasma membrane permeability	1377:1404	plasma membrane permeability	1377:1404	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	0	63	theme	Leonian	147:153	arg1	integrity					113:121	the cell membrane integrity	95:121	the cell membrane integrity of Phytophthora capsici Leonian	95:153	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	2	64	theme	cell	313:316	arg1	structure					327:335	the cell membrane structure	309:335	the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	309:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	9	65	theme	membrane	1384:1391	arg1	permeability					1393:1404	plasma membrane permeability	1377:1404	plasma membrane permeability	1377:1404	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	10	66	theme	composition	1664:1674	arg1	analysis					1676:1683	composition analysis	1664:1683	composition analysis of membrane structure and immunohistochemistry	1664:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	9	67	theme	O-CSLn-Cu	1346:1354	arg1	treatments					1356:1365	O-CSLn-Cu treatments	1346:1365	O-CSLn-Cu treatments	1346:1365	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	9	68	theme	membrane	1530:1537	arg1	fluidity					1539:1546	membrane fluidity	1530:1546	membrane fluidity	1530:1546	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	4	69	theme	cell	600:603	arg1	membrane					605:612	the fungal cell membrane	589:612	the fungal cell membrane	589:612	FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence.					
33766331	9	70	theme	intercellular	1434:1446	arg1	electrolytes					1448:1459	intercellular electrolytes	1434:1459	intercellular electrolytes	1434:1459	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	5	71	theme	transmission	871:882	arg1	TEM					905:907	TEM	905:907	TEM	905:907	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	71	theme	transmission	871:882	arg1	microscopy					893:902	transmission electron microscopy	871:902	transmission electron microscopy (TEM)	871:908	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	1	72	theme	drug	218:221	arg1	resistance					223:232	drug resistance	218:232	drug resistance in plant fungal diseases	218:257	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases.					
33766331	0	73	theme	antifungal	61:70	arg1	activity					72:79	antifungal activity	61:79	antifungal activity	61:79	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.					
33766331	5	74	theme	unilamellar	840:850	arg1	GUVs					862:865	GUVs	862:865	GUVs	862:865	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	5	74	theme	unilamellar	840:850	arg1	vesicles					852:859	giant unilamellar vesicles	834:859	giant unilamellar vesicles (GUVs)	834:866	O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM).					
33766331	8	75	theme	proteins	1242:1249	arg1	number					1223:1228	a large number	1215:1228	a large number of membrane proteins responsible for substance transport and biochemical reactions	1215:1311	Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated.					
33766331	8	76	theme	membrane	1233:1240	arg1	proteins					1242:1249	membrane proteins	1233:1249	membrane proteins responsible for substance transport and biochemical reactions	1233:1311	Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated.					
33766331	3	77	theme	nucleophilic	488:499	arg1	reaction					514:521	a nucleophilic substitution reaction	486:521	a nucleophilic substitution reaction	486:521	FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR.					
33766331	2	78	theme	chitosan	382:389	arg1	O-CSLn-Cu					408:416	O-CSLn-Cu	408:416	O-CSLn-Cu	408:416	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	2	78	theme	chitosan	382:389	arg1	complex					399:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex	348:405	the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu)	348:417	Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu).					
33766331	9	79	theme	significant	1411:1421	arg1	leakage					1423:1429	significant leakage	1411:1429	significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation	1411:1512	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	9	80	theme	electrolytes	1448:1459	arg1	leakage					1423:1429	significant leakage	1411:1429	significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation	1411:1512	Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity.					
33766331	10	81	theme	antimicrobial	1630:1642	arg1	agents					1644:1649	antimicrobial agents	1630:1649	antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry	1630:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	3	82	theme	substitution	501:512	arg1	reaction					514:521	a nucleophilic substitution reaction	486:521	a nucleophilic substitution reaction	486:521	FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR.					
33766331	10	83	theme	potential	1590:1598	arg1	target					1610:1615	a potential molecular target	1588:1615	a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry	1588:1730	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
33766331	10	83	theme	potential	1590:1598	arg1	aquaporin					1558:1566	aquaporin 10	1558:1569	aquaporin 10	1558:1569	Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.					
34119113	2	0	theme	AU/mL	473:477	arg1	CFS					479:481	80 AU/mL CFS	470:481	80 AU/mL CFS	470:481	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	1	1	theme	composite	172:180	arg1	film					182:185	a composite film	170:185	a composite film	170:185	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	2	theme	cell-free	263:271	arg1	supernatant					273:283	cell-free supernatant	263:283	cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E	263:341	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	2	theme	cell-free	263:271	arg1	CFS					286:288	CFS	286:288	CFS	286:288	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	0	3	theme	cell-free	85:93	arg1	supernatant					95:105	cell-free supernatant	85:105	cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1	85:152	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	5	4	used	used	1070:1073	arg2	films					1023:1027	BC-E films	1018:1027	BC-E films	1018:1027	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
34119113	2	5	theme	optimum	348:354	arg1	conditions					356:365	The optimum conditions	344:365	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity	344:445	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	5	theme	optimum	348:354	arg1	soak					456:459	the soak	452:459	the soak of BC in 80 AU/mL CFS	452:481	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	4	6	theme	BC-E	972:975	arg1	films					977:981	BC-E films	972:981	BC-E films presented better water resistance	972:1015	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	3	7	theme	mechanical	785:794	arg1	higher					834:839	higher	834:839	higher	834:839	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	7	theme	mechanical	785:794	arg1	properties					796:805	the mechanical properties	781:805	the mechanical properties of BC-E	781:813	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	0	8	theme	Enterococcus	127:138	arg1	TJUQ1					148:152	Enterococcus faecium TJUQ1	127:152	Enterococcus faecium TJUQ1	127:152	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	5	9	with	pack	1078:1081	arg1	log10 CFU/g					1109:1119	6.55 log10 CFU/g	1104:1119	6.55 log10 CFU/g of Listeria monocytogenes	1104:1145	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
34119113	2	10	theme	scanning	495:502	arg1	observation					524:534	scanning electron microscope observation	495:534	scanning electron microscope observation	495:534	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	11	theme	BC-E	570:573	arg1	denser					579:584	denser	579:584	denser	579:584	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	11	theme	BC-E	570:573	arg1	structure					557:565	the surface network structure	537:565	the surface network structure of BC-E	537:573	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	3	12	from	break	699:703	arg1	elongation					685:694	the elongation	681:694	the elongation at break of BC and BC-E	681:718	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	12	from	break	699:703	arg1	%					735:735	3.33 ± 0.89%	724:735	3.33 ± 0.89%	724:735	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	2	13	from	soak	456:459	arg1	CFS					479:481	80 AU/mL CFS	470:481	80 AU/mL CFS	470:481	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	0	14	dep	Enterococcus	127:138	arg1	faecium					140:146	faecium	140:146	faecium	140:146	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	2	15	theme	composite	394:402	arg1	film					404:407	the composite film	390:407	the composite film	390:407	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	4	16	theme	BC-E	891:894	arg1	ratio					882:886	The swelling ratio	869:886	The swelling ratio of BC-E (456.67 ± 7.20%)	869:911	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	4	16	theme	BC-E	891:894	arg1	lower					917:921	lower	917:921	lower	917:921	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	5	17	theme	BC-E	1018:1021	arg1	films					1023:1027	BC-E films	1018:1027	BC-E films	1018:1027	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
34119113	7	18	theme	control	1333:1339	arg1	groups					1341:1346	control groups	1333:1346	control groups	1333:1346	Similarly, total mesophilic bacterial levels in the ground meat decreased by 2.41 log10 CFU/g compared to control groups.					
34119113	3	19	dep	higher	834:839	arg1	P < 0.05					858:865	P < 0.05	858:865	P < 0.05	858:865	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	0	20	theme	Physical	0:7	arg1	properties					27:36	Physical and antibacterial properties	0:36	Physical and antibacterial properties of bacterial cellulose films	0:65	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	3	21	theme	BC-E	634:637	arg1	strength					615:622	The tensile strength	603:622	The tensile strength of BC and BC-E	603:637	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	21	theme	BC-E	634:637	arg1	4.65 ± 0.88 MPa					643:657	4.65 ± 0.88 MPa	643:657	4.65 ± 0.88 MPa	643:657	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	1	22	theme	bacterial	205:213	arg1	BC					226:227	BC	226:227	BC	226:227	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	22	theme	bacterial	205:213	arg1	cellulose					215:223	bacterial cellulose	205:223	bacterial cellulose (BC) of Gluconacetobacter xylinus	205:257	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	4	23	dep	lower	917:921	arg1	%					954:954	1377.78 ± 9.07%	940:954	1377.78 ± 9.07%	940:954	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	3	24	theme	BC-E	715:718	arg1	elongation					685:694	the elongation	681:694	the elongation at break of BC and BC-E	681:718	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	24	theme	BC-E	715:718	arg1	%					735:735	3.33 ± 0.89%	724:735	3.33 ± 0.89%	724:735	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	6	25	theme	storage	1164:1170	arg1	days					1156:1159	8 days	1154:1159	8 days of storage	1154:1170	After 8 days of storage, the number of bacteria decreased by 3.16 log10 CFU/g.					
34119113	2	26	theme	minimal	416:422	arg1	activity					438:445	a minimal antibacterial activity	414:445	a minimal antibacterial activity	414:445	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	0	27	theme	antibacterial	13:25	arg1	properties					27:36	Physical and antibacterial properties	0:36	Physical and antibacterial properties of bacterial cellulose films	0:65	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	1	28	theme	Enterococcus	294:305	arg1	TJUQ1					315:319	Enterococcus faecium TJUQ1	294:319	Enterococcus faecium TJUQ1	294:319	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	7	29	theme	ground	1279:1284	arg1	meat					1286:1289	the ground meat	1275:1289	the ground meat	1275:1289	Similarly, total mesophilic bacterial levels in the ground meat decreased by 2.41 log10 CFU/g compared to control groups.					
34119113	7	30	from	levels	1265:1270	arg1	meat					1286:1289	the ground meat	1275:1289	the ground meat	1275:1289	Similarly, total mesophilic bacterial levels in the ground meat decreased by 2.41 log10 CFU/g compared to control groups.					
34119113	0	31	theme	bacterial	41:49	arg1	films					61:65	bacterial cellulose films	41:65	bacterial cellulose films	41:65	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	3	32	theme	BC	708:709	arg1	elongation					685:694	the elongation	681:694	the elongation at break of BC and BC-E	681:718	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	32	theme	BC	708:709	arg1	%					735:735	3.33 ± 0.89%	724:735	3.33 ± 0.89%	724:735	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	4	33	theme	swelling	873:880	arg1	ratio					882:886	The swelling ratio	869:886	The swelling ratio of BC-E (456.67 ± 7.20%)	869:911	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	4	33	theme	swelling	873:880	arg1	lower					917:921	lower	917:921	lower	917:921	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	5	34	theme	ground	1087:1092	arg1	meat					1094:1097	the ground meat	1083:1097	the ground meat	1083:1097	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
34119113	2	35	theme	BC	464:465	arg1	conditions					356:365	The optimum conditions	344:365	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity	344:445	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	35	theme	BC	464:465	arg1	soak					456:459	the soak	452:459	the soak of BC in 80 AU/mL CFS	452:481	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	1	36	theme	TJUQ1	315:319	arg1	supernatant					273:283	cell-free supernatant	263:283	cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E	263:341	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	36	theme	TJUQ1	315:319	arg1	BC					226:227	BC	226:227	BC	226:227	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	36	theme	TJUQ1	315:319	arg1	cellulose					215:223	bacterial cellulose	205:223	bacterial cellulose (BC) of Gluconacetobacter xylinus	205:257	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	36	theme	TJUQ1	315:319	arg1	CFS					286:288	CFS	286:288	CFS	286:288	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	2	37	theme	microscope	513:522	arg1	observation					524:534	scanning electron microscope observation	495:534	scanning electron microscope observation	495:534	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	38	theme	film	404:407	arg1	preparation					375:385	the preparation	371:385	the preparation of the composite film	371:407	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	0	39	theme	films	61:65	arg1	properties					27:36	Physical and antibacterial properties	0:36	Physical and antibacterial properties of bacterial cellulose films	0:65	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	2	40	theme	electron	504:511	arg1	observation					524:534	scanning electron microscope observation	495:534	scanning electron microscope observation	495:534	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	6	41	theme	bacteria	1187:1194	arg1	number					1177:1182	the number	1173:1182	the number of bacteria	1173:1194	After 8 days of storage, the number of bacteria decreased by 3.16 log10 CFU/g.					
34119113	3	42	theme	tensile	607:613	arg1	strength					615:622	The tensile strength	603:622	The tensile strength of BC and BC-E	603:637	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	42	theme	tensile	607:613	arg1	4.65 ± 0.88 MPa					643:657	4.65 ± 0.88 MPa	643:657	4.65 ± 0.88 MPa	643:657	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	7	43	theme	mesophilic	1244:1253	arg1	levels					1265:1270	total mesophilic bacterial levels	1238:1270	total mesophilic bacterial levels in the ground meat	1238:1289	Similarly, total mesophilic bacterial levels in the ground meat decreased by 2.41 log10 CFU/g compared to control groups.					
34119113	0	44	theme	cellulose	51:59	arg1	films					61:65	bacterial cellulose films	41:65	bacterial cellulose films	41:65	Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1.					
34119113	2	45	with	conditions	356:365	arg1	activity					438:445	a minimal antibacterial activity	414:445	a minimal antibacterial activity	414:445	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	7	46	theme	bacterial	1255:1263	arg1	levels					1265:1270	total mesophilic bacterial levels	1238:1270	total mesophilic bacterial levels in the ground meat	1238:1289	Similarly, total mesophilic bacterial levels in the ground meat decreased by 2.41 log10 CFU/g compared to control groups.					
34119113	5	47	theme	AU/mL	1050:1054	arg1	CFS					1056:1058	320 AU/mL CFS	1046:1058	320 AU/mL CFS	1046:1058	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
34119113	3	48	theme	BC-E	810:813	arg1	higher					834:839	higher	834:839	higher	834:839	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	48	theme	BC-E	810:813	arg1	properties					796:805	the mechanical properties	781:805	the mechanical properties of BC-E	781:813	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	2	49	theme	network	549:555	arg1	denser					579:584	denser	579:584	denser	579:584	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	49	theme	network	549:555	arg1	structure					557:565	the surface network structure	537:565	the surface network structure of BC-E	537:573	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	50	theme	surface	541:547	arg1	denser					579:584	denser	579:584	denser	579:584	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	2	50	theme	surface	541:547	arg1	structure					557:565	the surface network structure	537:565	the surface network structure of BC-E	537:573	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	3	51	theme	BC	627:628	arg1	strength					615:622	The tensile strength	603:622	The tensile strength of BC and BC-E	603:637	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	3	51	theme	BC	627:628	arg1	4.65 ± 0.88 MPa					643:657	4.65 ± 0.88 MPa	643:657	4.65 ± 0.88 MPa	643:657	The tensile strength of BC and BC-E was 4.65 ± 0.88 MPa and 16.30 ± 0.92 MPa, the elongation at break of BC and BC-E was 3.33 ± 0.89% and 31.60 ± 1.15%, respectively, indicating the mechanical properties of BC-E were significantly higher than that of BC (P < 0.05).					
34119113	7	52	theme	total	1238:1242	arg1	levels					1265:1270	total mesophilic bacterial levels	1238:1270	total mesophilic bacterial levels in the ground meat	1238:1289	Similarly, total mesophilic bacterial levels in the ground meat decreased by 2.41 log10 CFU/g compared to control groups.					
34119113	4	53	theme	water	1000:1004	arg1	resistance					1006:1015	water resistance	1000:1015	water resistance	1000:1015	The swelling ratio of BC-E (456.67 ± 7.20%) was lower than that of BC (1377.78 ± 9.07%), demonstrating BC-E films presented better water resistance.					
34119113	1	54	theme	Gluconacetobacter	233:249	arg1	xylinus					251:257	Gluconacetobacter xylinus	233:257	Gluconacetobacter xylinus	233:257	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	55	theme	xylinus	251:257	arg1	supernatant					273:283	cell-free supernatant	263:283	cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E	263:341	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	55	theme	xylinus	251:257	arg1	BC					226:227	BC	226:227	BC	226:227	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	55	theme	xylinus	251:257	arg1	cellulose					215:223	bacterial cellulose	205:223	bacterial cellulose (BC) of Gluconacetobacter xylinus	205:257	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	55	theme	xylinus	251:257	arg1	CFS					286:288	CFS	286:288	CFS	286:288	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	1	56	dep	Enterococcus	294:305	arg1	faecium					307:313	faecium	307:313	faecium	307:313	In this study, a composite film was prepared with bacterial cellulose (BC) of Gluconacetobacter xylinus and cell-free supernatant (CFS) of Enterococcus faecium TJUQ1, which was named BC-E.					
34119113	2	57	theme	antibacterial	424:436	arg1	activity					438:445	a minimal antibacterial activity	414:445	a minimal antibacterial activity	414:445	The optimum conditions for the preparation of the composite film with a minimal antibacterial activity were the soak of BC in 80 AU/mL CFS for 6 h. By scanning electron microscope observation, the surface network structure of BC-E was denser than that of BC.					
34119113	5	58	theme	monocytogenes	1133:1145	arg1	log10 CFU/g					1109:1119	6.55 log10 CFU/g	1104:1119	6.55 log10 CFU/g of Listeria monocytogenes	1104:1145	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
34119113	5	59	theme	Listeria	1124:1131	arg1	monocytogenes					1133:1145	Listeria monocytogenes	1124:1145	Listeria monocytogenes	1124:1145	BC-E films were soaked with 320 AU/mL CFS, and then used to pack the ground meat with 6.55 log10 CFU/g of Listeria monocytogenes.					
33233097	4	0	theme	high-throughput	639:653	arg1	sequencing					655:664	high-throughput sequencing	639:664	high-throughput sequencing of 16S rRNA	639:676	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	3	1	theme	detailed	461:468	arg1	mechanism					470:478	the detailed mechanism	457:478	the detailed mechanism	457:478	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	8	2	from	metabolites	1308:1318	arg1	metabolism					1350:1359	gut mediating host glucose metabolism	1323:1359	gut mediating host glucose metabolism	1323:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	6	3	theme	treated	946:952	arg1	rats					954:957	FOs treated rats	942:957	FOs treated rats	942:957	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	0	4	theme	diabetic	81:88	arg1	syndrome					90:97	diabetic syndrome	81:97	diabetic syndrome	81:97	Feruloylated oligosaccharides and ferulic acid alter gut microbiome to alleviate diabetic syndrome.					
33233097	10	5	theme	supplements	1726:1736	arg1	development					1703:1713	the development	1699:1713	the development of dietary supplements for diabetic patients	1699:1758	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	8	6	theme	main	1287:1290	arg1	acids					1229:1233	fatty acids	1223:1233	fatty acids (SCFAs)	1223:1241	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	6	theme	main	1287:1290	arg1	metabolites					1308:1318	the main bacterial lipid metabolites	1283:1318	the main bacterial lipid metabolites in gut mediating host glucose metabolism	1283:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	6	7	theme	FOs	942:944	arg1	rats					954:957	FOs treated rats	942:957	FOs treated rats	942:957	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	9	8	theme	gut	1557:1559	arg1	microbiome					1561:1570	gut microbiome	1557:1570	gut microbiome	1557:1570	Our findings indicated that FOs and FA attenuated diabetic syndrome in rats most likely by modulating the composition and metabolism of gut microbiome.					
33233097	10	9	theme	diabetic	1742:1749	arg1	patients					1751:1758	diabetic patients	1742:1758	diabetic patients	1742:1758	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	9	10	theme	diabetic	1471:1478	arg1	syndrome					1480:1487	diabetic syndrome	1471:1487	diabetic syndrome in rats	1471:1495	Our findings indicated that FOs and FA attenuated diabetic syndrome in rats most likely by modulating the composition and metabolism of gut microbiome.					
33233097	9	11	from	syndrome	1480:1487	arg1	rats					1492:1495	rats	1492:1495	rats	1492:1495	Our findings indicated that FOs and FA attenuated diabetic syndrome in rats most likely by modulating the composition and metabolism of gut microbiome.					
33233097	5	12	theme	Phascolarctobacterium	867:887	arg1	abundance					841:849	the abundance	837:849	the abundance of Akkermansia, Phascolarctobacterium and Turicibacter	837:904	Our results showed that FOs reduced the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio, but increased the abundance of Akkermansia, Phascolarctobacterium and Turicibacter.					
33233097	10	13	theme	foods	1670:1674	arg1	effect					1649:1654	the anti-diabetes effect	1631:1654	the anti-diabetes effect of functional foods	1631:1674	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	2	14	theme	microbiome	260:269	arg1	composition					241:251	the composition	237:251	the composition of gut microbiome	237:269	Additionally, increasing evidence showed that the composition of gut microbiome is highly associated with the outcome of T2D therapy.					
33233097	6	15	theme	microbiome	928:937	arg1	structure					911:919	The structure	907:919	The structure of gut microbiome in FOs treated rats	907:957	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	6	15	theme	microbiome	928:937	arg1	similar					963:969	similar	963:969	similar	963:969	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	2	16	theme	gut	256:258	arg1	microbiome					260:269	gut microbiome	256:269	gut microbiome	256:269	Additionally, increasing evidence showed that the composition of gut microbiome is highly associated with the outcome of T2D therapy.					
33233097	5	17	theme	Oscillibacter	789:801	arg1	abundance					747:755	the abundance	743:755	the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio	743:820	Our results showed that FOs reduced the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio, but increased the abundance of Akkermansia, Phascolarctobacterium and Turicibacter.					
33233097	7	18	theme	Blautia	1123:1129	arg1	growth					1100:1105	the growth	1096:1105	the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium	1096:1190	Likewise, FA decreased the portion of Lactobacillus, Ruminococcus, but promoted the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium.					
33233097	4	19	from	metabolome	602:611	arg1	rats					625:628	diabetic rats	616:628	diabetic rats	616:628	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	1	20	theme	type	168:171	arg1	T2D					185:187	T2D	185:187	T2D	185:187	Gut microbiome has been proven to be involved in the development of type 2 diabetes (T2D).					
33233097	1	20	theme	type	168:171	arg1	diabetes					175:182	type 2 diabetes	168:182	type 2 diabetes (T2D)	168:188	Gut microbiome has been proven to be involved in the development of type 2 diabetes (T2D).					
33233097	7	21	theme	Parabacteroides	1150:1164	arg1	growth					1100:1105	the growth	1096:1105	the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium	1096:1190	Likewise, FA decreased the portion of Lactobacillus, Ruminococcus, but promoted the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium.					
33233097	10	22	theme	dietary	1718:1724	arg1	supplements					1726:1736	dietary supplements	1718:1736	dietary supplements	1718:1736	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	4	23	theme	gas	682:684	arg1	chromatography					686:699	gas chromatography	682:699	gas chromatography (GC)	682:704	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	4	23	theme	gas	682:684	arg1	GC					702:703	GC	702:703	GC	702:703	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	4	24	theme	diabetic	616:623	arg1	rats					625:628	diabetic rats	616:628	diabetic rats	616:628	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	0	25	theme	Feruloylated	0:11	arg1	oligosaccharides					13:28	Feruloylated oligosaccharides	0:28	Feruloylated oligosaccharides	0:28	Feruloylated oligosaccharides and ferulic acid alter gut microbiome to alleviate diabetic syndrome.					
33233097	6	26	theme	gut	924:926	arg1	microbiome					928:937	gut microbiome	924:937	gut microbiome	924:937	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	1	27	theme	Gut	100:102	arg1	microbiome					104:113	Gut microbiome	100:113	Gut microbiome	100:113	Gut microbiome has been proven to be involved in the development of type 2 diabetes (T2D).					
33233097	1	28	theme	diabetes	175:182	arg1	development					153:163	the development	149:163	the development of type 2 diabetes (T2D)	149:188	Gut microbiome has been proven to be involved in the development of type 2 diabetes (T2D).					
33233097	0	29	theme	ferulic	34:40	arg1	acid					42:45	ferulic acid	34:45	ferulic acid	34:45	Feruloylated oligosaccharides and ferulic acid alter gut microbiome to alleviate diabetic syndrome.					
33233097	6	30	from	structure	911:919	arg1	rats					954:957	FOs treated rats	942:957	FOs treated rats	942:957	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	8	31	theme	host	1337:1340	arg1	metabolism					1350:1359	gut mediating host glucose metabolism	1323:1359	gut mediating host glucose metabolism	1323:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	5	32	theme	Turicibacter	893:904	arg1	abundance					841:849	the abundance	837:849	the abundance of Akkermansia, Phascolarctobacterium and Turicibacter	837:904	Our results showed that FOs reduced the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio, but increased the abundance of Akkermansia, Phascolarctobacterium and Turicibacter.					
33233097	4	33	from	microbiome	579:588	arg1	rats					625:628	diabetic rats	616:628	diabetic rats	616:628	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	4	34	theme	gut	575:577	arg1	microbiome					579:588	gut microbiome	575:588	gut microbiome	575:588	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	3	35	theme	feruloylated	357:368	arg1	oligosaccharides					370:385	feruloylated oligosaccharides	357:385	feruloylated oligosaccharides (FOs)	357:391	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	3	35	theme	feruloylated	357:368	arg1	FOs					388:390	FOs	388:390	FOs	388:390	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	8	36	theme	fatty	1223:1227	arg1	metabolites					1308:1318	the main bacterial lipid metabolites	1283:1318	the main bacterial lipid metabolites in gut mediating host glucose metabolism	1283:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	36	theme	fatty	1223:1227	arg1	SCFAs					1236:1240	SCFAs	1236:1240	SCFAs	1236:1240	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	36	theme	fatty	1223:1227	arg1	acids					1229:1233	fatty acids	1223:1233	fatty acids (SCFAs)	1223:1241	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	37	theme	gut	1323:1325	arg1	metabolism					1350:1359	gut mediating host glucose metabolism	1323:1359	gut mediating host glucose metabolism	1323:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	38	theme	short-chain	1211:1221	arg1	metabolites					1308:1318	the main bacterial lipid metabolites	1283:1318	the main bacterial lipid metabolites in gut mediating host glucose metabolism	1283:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	38	theme	short-chain	1211:1221	arg1	SCFAs					1236:1240	SCFAs	1236:1240	SCFAs	1236:1240	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	38	theme	short-chain	1211:1221	arg1	acids					1229:1233	fatty acids	1223:1233	fatty acids (SCFAs)	1223:1241	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	10	39	theme	anti-diabetes	1635:1647	arg1	effect					1649:1654	the anti-diabetes effect	1631:1654	the anti-diabetes effect of functional foods	1631:1674	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	8	40	theme	mediating	1327:1335	arg1	metabolism					1350:1359	gut mediating host glucose metabolism	1323:1359	gut mediating host glucose metabolism	1323:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	5	41	theme	Desulfovibrio	808:820	arg1	abundance					747:755	the abundance	743:755	the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio	743:820	Our results showed that FOs reduced the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio, but increased the abundance of Akkermansia, Phascolarctobacterium and Turicibacter.					
33233097	5	42	theme	Ruminococcus	775:786	arg1	abundance					747:755	the abundance	743:755	the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio	743:820	Our results showed that FOs reduced the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio, but increased the abundance of Akkermansia, Phascolarctobacterium and Turicibacter.					
33233097	3	43	theme	ferulic	397:403	arg1	FA					411:412	FA	411:412	FA	411:412	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	3	43	theme	ferulic	397:403	arg1	acid					405:408	ferulic acid	397:408	ferulic acid (FA)	397:413	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	7	44	theme	Lactobacillus	1054:1066	arg1	portion					1043:1049	the portion	1039:1049	the portion of Lactobacillus, Ruminococcus,	1039:1081	Likewise, FA decreased the portion of Lactobacillus, Ruminococcus, but promoted the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium.					
33233097	2	45	theme	increasing	205:214	arg1	evidence					216:223	increasing evidence	205:223	increasing evidence	205:223	Additionally, increasing evidence showed that the composition of gut microbiome is highly associated with the outcome of T2D therapy.					
33233097	3	46	theme	diabetic	426:433	arg1	syndrome					435:442	diabetic syndrome	426:442	diabetic syndrome in rats	426:450	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	9	47	dep	composition	1527:1537	arg1	the					1523:1525	the	1523:1525	the	1523:1525	Our findings indicated that FOs and FA attenuated diabetic syndrome in rats most likely by modulating the composition and metabolism of gut microbiome.					
33233097	8	48	theme	lipid	1302:1306	arg1	acids					1229:1233	fatty acids	1223:1233	fatty acids (SCFAs)	1223:1241	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	48	theme	lipid	1302:1306	arg1	metabolites					1308:1318	the main bacterial lipid metabolites	1283:1318	the main bacterial lipid metabolites in gut mediating host glucose metabolism	1283:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	49	theme	glucose	1342:1348	arg1	metabolism					1350:1359	gut mediating host glucose metabolism	1323:1359	gut mediating host glucose metabolism	1323:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	50	theme	bacterial	1292:1300	arg1	acids					1229:1233	fatty acids	1223:1233	fatty acids (SCFAs)	1223:1241	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	50	theme	bacterial	1292:1300	arg1	metabolites					1308:1318	the main bacterial lipid metabolites	1283:1318	the main bacterial lipid metabolites in gut mediating host glucose metabolism	1283:1359	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	2	51	theme	therapy	316:322	arg1	outcome					301:307	the outcome	297:307	the outcome of T2D therapy	297:322	Additionally, increasing evidence showed that the composition of gut microbiome is highly associated with the outcome of T2D therapy.					
33233097	0	52	theme	gut	53:55	arg1	microbiome					57:66	gut microbiome	53:66	gut microbiome	53:66	Feruloylated oligosaccharides and ferulic acid alter gut microbiome to alleviate diabetic syndrome.					
33233097	8	53	theme	FA	1407:1408	arg1	treatment					1410:1418	FA treatment	1407:1418	FA treatment	1407:1418	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	6	54	with	similar	963:969	arg1	rats					1010:1013	diabetic rats	1001:1013	healthy rats rather than diabetic rats	976:1013	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	6	54	with	similar	963:969	arg1	rats					984:987	healthy rats	976:987	healthy rats rather than diabetic rats	976:1013	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	3	55	from	syndrome	435:442	arg1	rats					447:450	rats	447:450	rats	447:450	Previously we demonstrated that feruloylated oligosaccharides (FOs) and ferulic acid (FA) alleviated diabetic syndrome in rats, but the detailed mechanism has not been explored yet.					
33233097	2	56	theme	T2D	312:314	arg1	therapy					316:322	T2D therapy	312:322	T2D therapy	312:322	Additionally, increasing evidence showed that the composition of gut microbiome is highly associated with the outcome of T2D therapy.					
33233097	4	57	dep	microbiome	579:588	arg1	the					571:573	the	571:573	the	571:573	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	4	58	theme	rRNA	673:676	arg1	chromatography					686:699	gas chromatography	682:699	gas chromatography (GC)	682:704	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	4	58	theme	rRNA	673:676	arg1	GC					702:703	GC	702:703	GC	702:703	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	4	58	theme	rRNA	673:676	arg1	sequencing					655:664	high-throughput sequencing	639:664	high-throughput sequencing of 16S rRNA	639:676	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	6	59	theme	diabetic	1001:1008	arg1	rats					1010:1013	diabetic rats	1001:1013	healthy rats rather than diabetic rats	976:1013	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	4	60	theme	16S	669:671	arg1	rRNA					673:676	16S rRNA	669:676	16S rRNA	669:676	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	8	61	theme	branched-chain	1247:1260	arg1	acids					1268:1272	branched-chain fatty acids	1247:1272	branched-chain fatty acids (BCFAs)	1247:1280	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	61	theme	branched-chain	1247:1260	arg1	BCFAs					1275:1279	BCFAs	1275:1279	BCFAs	1275:1279	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	6	62	theme	healthy	976:982	arg1	rats					984:987	healthy rats	976:987	healthy rats rather than diabetic rats	976:1013	The structure of gut microbiome in FOs treated rats was similar with healthy rats rather than diabetic rats.					
33233097	9	63	theme	microbiome	1561:1570	arg1	metabolism					1543:1552	metabolism	1543:1552	metabolism	1543:1552	Our findings indicated that FOs and FA attenuated diabetic syndrome in rats most likely by modulating the composition and metabolism of gut microbiome.					
33233097	9	63	theme	microbiome	1561:1570	arg1	composition					1527:1537	composition	1527:1537	composition	1527:1537	Our findings indicated that FOs and FA attenuated diabetic syndrome in rats most likely by modulating the composition and metabolism of gut microbiome.					
33233097	5	64	theme	Lactobacillus	760:772	arg1	abundance					747:755	the abundance	743:755	the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio	743:820	Our results showed that FOs reduced the abundance of Lactobacillus, Ruminococcus, Oscillibacter, and Desulfovibrio, but increased the abundance of Akkermansia, Phascolarctobacterium and Turicibacter.					
33233097	10	65	theme	functional	1659:1668	arg1	foods					1670:1674	functional foods	1659:1674	functional foods	1659:1674	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	4	66	theme	related	594:600	arg1	metabolome					602:611	related metabolome	594:611	related metabolome	594:611	In this study we strived to characterize how FOs and FA altered the gut microbiome and related metabolome in diabetic rats by using high-throughput sequencing of 16S rRNA and gas chromatography (GC).					
33233097	10	67	theme	new	1589:1591	arg1	insight					1593:1599	new insight	1589:1599	new insight into the mechanism underlying the anti-diabetes effect of functional foods	1589:1674	The study gives new insight into the mechanism underlying the anti-diabetes effect of functional foods as well as facilitates the development of dietary supplements for diabetic patients.					
33233097	7	68	theme	Phascolarctobacterium	1170:1190	arg1	growth					1100:1105	the growth	1096:1105	the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium	1096:1190	Likewise, FA decreased the portion of Lactobacillus, Ruminococcus, but promoted the growth of Bacteroides, Blautia, Faecalibacterium, Parabacteroides and Phascolarctobacterium.					
33233097	8	69	theme	fatty	1262:1266	arg1	acids					1268:1272	branched-chain fatty acids	1247:1272	branched-chain fatty acids (BCFAs)	1247:1280	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
33233097	8	69	theme	fatty	1262:1266	arg1	BCFAs					1275:1279	BCFAs	1275:1279	BCFAs	1275:1279	Additionally, the short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs), the main bacterial lipid metabolites in gut mediating host glucose metabolism, was dramatically elevated along with FOs and FA treatment.					
34673798	4	0	theme	genes	981:985	arg1	%					950:950	43.6-52.1%	941:950	43.6-52.1% of total glycoside hydrolase genes	941:985	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	0	theme	genes	981:985	arg1	activity					1002:1009	34.6-47.1% activity	991:1009	34.6-47.1% activity	991:1009	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	0	theme	genes	981:985	arg1	genes					981:985	total glycoside hydrolase genes	955:985	total glycoside hydrolase genes	955:985	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	2	1	theme	intestinal	376:385	arg1	microbiota					387:396	the intestinal microbiota	372:396	the intestinal microbiota	372:396	However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented.					
34673798	4	2	theme	chickens	1041:1048	arg1	meta-transcriptomes					1018:1036	the meta-transcriptomes	1014:1036	the meta-transcriptomes of chickens in the different dietary groups	1014:1080	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	0	3	theme	organisms	101:109	arg1	composition					45:55	composition	45:55	composition	45:55	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	0	3	theme	organisms	101:109	arg1	activities					61:70	activities	61:70	activities	61:70	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	1	4	theme	effective	175:183	arg1	prebiotic					185:193	a highly effective prebiotic	166:193	a highly effective prebiotic	166:193	Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens.					
34673798	1	4	theme	effective	175:183	arg1	Inulin					156:161	Inulin	156:161	Inulin	156:161	Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens.					
34673798	4	5	theme	different	1057:1065	arg1	groups					1075:1080	the different dietary groups	1053:1080	the different dietary groups	1053:1080	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	3	6	theme	broiler	823:829	arg1	chickens					831:838	broiler chickens	823:838	broiler chickens	823:838	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	0	7	theme	cecal	118:122	arg1	microbiota					124:133	the cecal microbiota	114:133	the cecal microbiota of broiler chickens	114:153	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	4	8	theme	others	1204:1209	arg1	members					1092:1098	members	1092:1098	members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others	1092:1209	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	9	theme	Bacteroides	866:876	arg1	members					855:861	members	855:861	members of Bacteroides	855:876	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	9	theme	Bacteroides	866:876	arg1	CMOs					922:925	the most abundant non-starch degrading CMOs	883:925	the most abundant non-starch degrading CMOs	883:925	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	2	10	from	effects	350:356	arg1	members					361:367	members	361:367	members of the intestinal microbiota	361:396	However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented.					
34673798	3	11	theme	transcriptomic	558:571	arg1	analysis					573:580	transcriptomic analysis	558:580	transcriptomic analysis	558:580	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	12	theme	cecal	803:807	arg1	microbiota					809:818	the cecal microbiota	799:818	the cecal microbiota of broiler chickens	799:838	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	6	13	theme	Inulin	1363:1368	arg1	supplementation					1370:1384	Inulin supplementation	1363:1384	Inulin supplementation at three levels	1363:1400	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	0	14	from	activities	61:70	arg1	microbiota					124:133	the cecal microbiota	114:133	the cecal microbiota of broiler chickens	114:153	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	6	15	theme	%	1479:1479	arg1	dosage					1518:1523	the most optimal dosage	1501:1523	the most optimal dosage for bifidobacterial activity	1501:1552	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	6	15	theme	%	1479:1479	arg1	level					1481:1485	2% level	1478:1485	2% level	1478:1485	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	3	16	theme	analysis	573:580	arg1	combination					510:520	A combination	508:520	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis	508:580	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	17	dep	%	674:674	arg1	2					667:667	2	667:667	2	667:667	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	17	dep	%	674:674	arg1	4					673:673	4	673:673	4	673:673	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	6	18	theme	2	1478:1478	arg1	%					1479:1479	%	1479:1479	%	1479:1479	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	3	19	theme	sequencing	543:552	arg1	combination					510:520	A combination	508:520	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis	508:580	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	4	20	theme	non-starch	901:910	arg1	members					855:861	members	855:861	members of Bacteroides	855:876	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	20	theme	non-starch	901:910	arg1	CMOs					922:925	the most abundant non-starch degrading CMOs	883:925	the most abundant non-starch degrading CMOs	883:925	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	0	21	from	composition	45:55	arg1	microbiota					124:133	the cecal microbiota	114:133	the cecal microbiota of broiler chickens	114:153	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	5	22	from	inclusion	1262:1270	arg1	diet					1285:1288	the basal diet	1275:1288	the basal diet	1275:1288	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
34673798	6	23	theme	bifidobacterial	1529:1543	arg1	activity					1545:1552	bifidobacterial activity	1529:1552	bifidobacterial activity	1529:1552	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	6	24	from	levels	1395:1400	arg1	supplementation					1370:1384	Inulin supplementation	1363:1384	Inulin supplementation at three levels	1363:1400	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	0	25	theme	inulin	8:13	arg1	supplementation					15:29	Dietary inulin supplementation	0:29	Dietary inulin supplementation	0:29	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	4	26	theme	hydrolase	971:979	arg1	genes					981:985	total glycoside hydrolase genes	955:985	total glycoside hydrolase genes	955:985	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	27	theme	glycoside	961:969	arg1	hydrolase					971:979	total glycoside hydrolase	955:979	total glycoside hydrolase genes	955:985	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	28	theme	Alistipes	1132:1140	arg1	members					1092:1098	members	1092:1098	members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others	1092:1209	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	0	29	theme	Dietary	0:6	arg1	supplementation					15:29	Dietary inulin supplementation	0:29	Dietary inulin supplementation	0:29	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	3	30	dep	inulin	676:681	arg1	bacitracin					694:703	bacitracin	694:703	bacitracin	694:703	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	30	dep	inulin	676:681	arg1	%					674:674	%	674:674	%	674:674	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	1	31	theme	attractive	202:211	arg1	alternative					213:223	an attractive alternative	199:223	an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens	199:315	Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens.					
34673798	6	32	theme	Prevotella	1442:1451	arg1	activities					1415:1424	the activities	1411:1424	the activities of Bacteroides, Prevotella and Bifidobacterium	1411:1471	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	4	33	from	meta-transcriptomes	1018:1036	arg1	groups					1075:1080	the different dietary groups	1053:1080	the different dietary groups	1053:1080	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	0	34	theme	chickens	146:153	arg1	microbiota					124:133	the cecal microbiota	114:133	the cecal microbiota of broiler chickens	114:153	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	3	35	from	effects	610:616	arg1	activities					739:748	activities	739:748	activities	739:748	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	35	from	effects	610:616	arg1	diversity					725:733	diversity	725:733	diversity	725:733	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	35	from	effects	610:616	arg1	composition					712:722	the composition	708:722	the composition	708:722	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	36	used	used	586:589	arg2	combination					510:520	A combination	508:520	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis	508:580	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	5	37	dep	composition	1323:1333	arg1	the					1319:1321	the	1319:1321	the	1319:1321	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
34673798	0	38	theme	broiler	138:144	arg1	chickens					146:153	broiler chickens	138:153	broiler chickens	138:153	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	3	39	theme	16S	525:527	arg1	sequencing					543:552	16S rRNA Illumina sequencing	525:552	16S rRNA Illumina sequencing	525:552	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	4	40	theme	34.6-47.1	991:999	arg1	%					1000:1000	%	1000:1000	%	1000:1000	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	6	41	theme	Bifidobacterium	1457:1471	arg1	activities					1415:1424	the activities	1411:1424	the activities of Bacteroides, Prevotella and Bifidobacterium	1411:1471	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	4	42	theme	Parabacteroides	1103:1117	arg1	members					1092:1098	members	1092:1098	members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others	1092:1209	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	1	43	theme	antibiotic	228:237	arg1	promoters					246:254	antibiotic growth promoters	228:254	antibiotic growth promoters	228:254	Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens.					
34673798	3	44	theme	organisms	779:787	arg1	activities					739:748	activities	739:748	activities	739:748	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	44	theme	organisms	779:787	arg1	diversity					725:733	diversity	725:733	diversity	725:733	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	44	theme	organisms	779:787	arg1	composition					712:722	the composition	708:722	the composition	708:722	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	1	45	theme	growth	239:244	arg1	promoters					246:254	antibiotic growth promoters	228:254	antibiotic growth promoters	228:254	Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens.					
34673798	2	46	theme	chickens	479:486	arg1	importance					428:437	their importance	422:437	their importance for energy metabolism	422:459	However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented.					
34673798	2	46	theme	chickens	479:486	arg1	health					469:474	the health	465:474	the health of chickens	465:486	However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented.					
34673798	0	47	dep	composition	45:55	arg1	the					41:43	the	41:43	the	41:43	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	4	48	theme	%	1000:1000	arg1	activity					1002:1009	34.6-47.1% activity	991:1009	34.6-47.1% activity	991:1009	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	1	49	from	health	298:303	arg1	chickens					308:315	chickens	308:315	chickens	308:315	Inulin is a highly effective prebiotic and an attractive alternative to antibiotic growth promoters for increasing production and maintaining health in chickens.					
34673798	4	50	dep	Faecalibacterium	1183:1198	arg1	Barnesiella					1156:1166	Barnesiella	1156:1166	Barnesiella	1156:1166	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	50	dep	Faecalibacterium	1183:1198	arg1	Blastocystis					1169:1180	Clostridium, Barnesiella, Blastocystis, Faecalibacterium	1143:1198	Blastocystis	1169:1180	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	5	51	theme	basal	1279:1283	arg1	diet					1285:1288	the basal diet	1275:1288	the basal diet	1275:1288	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
34673798	3	52	theme	carbohydrate-metabolizing	753:777	arg1	CMOs					790:793	CMOs	790:793	CMOs	790:793	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	52	theme	carbohydrate-metabolizing	753:777	arg1	organisms					779:787	carbohydrate-metabolizing organisms	753:787	carbohydrate-metabolizing organisms (CMOs)	753:794	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	4	53	theme	abundant	892:899	arg1	members					855:861	members	855:861	members of Bacteroides	855:876	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	53	theme	abundant	892:899	arg1	CMOs					922:925	the most abundant non-starch degrading CMOs	883:925	the most abundant non-starch degrading CMOs	883:925	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	6	54	theme	optimal	1510:1516	arg1	dosage					1518:1523	the most optimal dosage	1501:1523	the most optimal dosage for bifidobacterial activity	1501:1552	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	6	54	theme	optimal	1510:1516	arg1	level					1481:1485	2% level	1478:1485	2% level	1478:1485	Inulin supplementation at three levels promoted the activities of Bacteroides, Prevotella and Bifidobacterium, and 2% level appears to be the most optimal dosage for bifidobacterial activity.					
34673798	3	55	theme	basal	648:652	arg1	diet					654:657	a corn-based basal diet	635:657	a corn-based basal diet	635:657	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	4	56	theme	Faecalibacterium	1183:1198	arg1	members					1092:1098	members	1092:1098	members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others	1092:1209	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	57	theme	dietary	1067:1073	arg1	groups					1075:1080	the different dietary groups	1053:1080	the different dietary groups	1053:1080	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	5	58	theme	CMOs	1357:1360	arg1	diversity					1338:1346	diversity	1338:1346	diversity	1338:1346	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
34673798	5	58	theme	CMOs	1357:1360	arg1	composition					1323:1333	composition	1323:1333	composition	1323:1333	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
34673798	3	59	theme	Illumina	534:541	arg1	sequencing					543:552	16S rRNA Illumina sequencing	525:552	16S rRNA Illumina sequencing	525:552	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	3	60	theme	chickens	831:838	arg1	microbiota					809:818	the cecal microbiota	799:818	the cecal microbiota of broiler chickens	799:838	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	0	61	theme	carbohydrate-metabolizing	75:99	arg1	organisms					101:109	carbohydrate-metabolizing organisms	75:109	carbohydrate-metabolizing organisms	75:109	Dietary inulin supplementation modulates the composition and activities of carbohydrate-metabolizing organisms in the cecal microbiota of broiler chickens.					
34673798	4	62	theme	degrading	912:920	arg1	members					855:861	members	855:861	members of Bacteroides	855:876	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	4	62	theme	degrading	912:920	arg1	CMOs					922:925	the most abundant non-starch degrading CMOs	883:925	the most abundant non-starch degrading CMOs	883:925	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	2	63	theme	energy	443:448	arg1	metabolism					450:459	energy metabolism	443:459	energy metabolism	443:459	However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented.					
34673798	3	64	theme	rRNA	529:532	arg1	sequencing					543:552	16S rRNA Illumina sequencing	525:552	16S rRNA Illumina sequencing	525:552	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	5	65	theme	Inulin	1240:1245	arg1	inclusion					1262:1270	Inulin and bacitracin inclusion	1240:1270	Inulin and bacitracin inclusion in the basal diet	1240:1288	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
34673798	4	66	theme	total	955:959	arg1	hydrolase					971:979	total glycoside hydrolase	955:979	total glycoside hydrolase genes	955:985	We found that members of Bacteroides were the most abundant non-starch degrading CMOs, contributing 43.6-52.1% of total glycoside hydrolase genes and 34.6-47.1% activity to the meta-transcriptomes of chickens in the different dietary groups, although members of Parabacteroides, Prevotella, Alistipes, Clostridium, Barnesiella, Blastocystis, Faecalibacterium and others were also actively involved.					
34673798	3	67	theme	corn-based	637:646	arg1	diet					654:657	a corn-based basal diet	635:657	a corn-based basal diet	635:657	A combination of 16S rRNA Illumina sequencing and transcriptomic analysis was used to investigate the effects of supplementing a corn-based basal diet with 1, 2, or 4% inulin or 400 ppm bacitracin on the composition, diversity and activities of carbohydrate-metabolizing organisms (CMOs) in the cecal microbiota of broiler chickens.					
34673798	2	68	theme	microbiota	387:396	arg1	members					361:367	members	361:367	members of the intestinal microbiota	361:396	However, how inulin elicits its effects on members of the intestinal microbiota is unknown, even though their importance for energy metabolism and the health of chickens is well documented.					
34673798	5	69	theme	bacitracin	1251:1260	arg1	inclusion					1262:1270	Inulin and bacitracin inclusion	1240:1270	Inulin and bacitracin inclusion in the basal diet	1240:1288	Inulin and bacitracin inclusion in the basal diet did not change significantly the composition or diversity of these CMOs.					
32756631	9	0	theme	flame	1785:1789	arg1	mechanisms					1801:1810	the possible flame retardant mechanisms	1772:1810	the possible flame retardant mechanisms in condensed and gaseous phases	1772:1842	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	0	1	theme	bio-based	144:152	arg1	complex					170:176	a bio-based polyelectrolyte complex	142:176	a bio-based polyelectrolyte complex formed by chitosan and phytic acid	142:211	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	1	2	theme	deteriorating	345:357	arg1	properties					370:379	deteriorating mechanical properties	345:379	deteriorating mechanical properties	345:379	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	11	3	theme	facile	2057:2062	arg1	retardants					2109:2118	eco-friendly flame retardants	2090:2118	eco-friendly flame retardants	2090:2118	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	3	theme	facile	2057:2062	arg1	technology					2075:2084	the facile processing technology	2053:2084	the facile processing technology	2053:2084	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	3	theme	facile	2057:2062	arg1	result					2045:2050	a result	2043:2050	a result	2043:2050	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	3	4	from	PEC	816:818	arg1	ratio					785:789	the ratio	781:789	the ratio of each component in the PEC	781:818	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	0	5	theme	complex	170:176	arg1	introduction					126:137	introduction	126:137	introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid	126:211	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	1	6	theme	human	407:411	arg1	health					413:418	human health	407:418	human health	407:418	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	10	7	theme	bonds	1981:1985	arg1	movement					2015:2022	the PVA molecular chain movement	1991:2022	the PVA molecular chain movement blocked by PEC	1991:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	7	theme	bonds	1981:1985	arg1	backbones					1933:1941	their harder backbones	1920:1941	their harder backbones of chitosan	1920:1953	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	7	theme	bonds	1981:1985	arg1	formation					1956:1964	formation	1956:1964	formation of phosphonate bonds	1956:1985	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	9	8	theme	condensed	1815:1823	arg1	phases					1837:1842	condensed and gaseous phases	1815:1842	condensed and gaseous phases	1815:1842	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	11	9	theme	eco-friendly	2090:2101	arg1	retardants					2109:2118	eco-friendly flame retardants	2090:2118	eco-friendly flame retardants	2090:2118	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	9	theme	eco-friendly	2090:2101	arg1	technology					2075:2084	the facile processing technology	2053:2084	the facile processing technology	2053:2084	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	9	theme	eco-friendly	2090:2101	arg1	result					2045:2050	a result	2043:2050	a result	2043:2050	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	9	10	theme	gaseous	1829:1835	arg1	phases					1837:1842	condensed and gaseous phases	1815:1842	condensed and gaseous phases	1815:1842	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	10	11	theme	chitosan	1946:1953	arg1	movement					2015:2022	the PVA molecular chain movement	1991:2022	the PVA molecular chain movement blocked by PEC	1991:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	11	theme	chitosan	1946:1953	arg1	backbones					1933:1941	their harder backbones	1920:1941	their harder backbones of chitosan	1920:1953	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	11	theme	chitosan	1946:1953	arg1	formation					1956:1964	formation	1956:1964	formation of phosphonate bonds	1956:1985	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	7	12	theme	heat	1352:1355	arg1	THR					1366:1368	THR	1366:1368	THR	1366:1368	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	12	theme	heat	1352:1355	arg1	release					1357:1363	the total heat release	1342:1363	the total heat release (THR)	1342:1369	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	10	13	theme	molecular	1999:2007	arg1	movement					2015:2022	the PVA molecular chain movement	1991:2022	the PVA molecular chain movement blocked by PEC	1991:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	13	theme	molecular	1999:2007	arg1	backbones					1933:1941	their harder backbones	1920:1941	their harder backbones of chitosan	1920:1953	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	1	14	from	problems	239:246	arg1	process					255:261	the process	251:261	the process of the flame retardant treatment of poly vinyl alcohol (PVA)	251:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	5	15	theme	PEC	1013:1015	arg1	addition					1001:1008	the addition	997:1008	the addition of PEC	997:1015	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	2	16	theme	polyelectrolyte	495:509	arg1	complex					511:517	a green and eco-friendly bio-based polyelectrolyte complex	460:517	a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid	460:558	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	2	16	theme	polyelectrolyte	495:509	arg1	PEC					520:522	PEC	520:522	PEC	520:522	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	3	17	theme	retardant	730:738	arg1	properties					740:749	the mechanical and flame retardant properties	705:749	the mechanical and flame retardant properties	705:749	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	0	18	theme	alcohol	114:120	arg1	properties					89:98	the flame retardant and mechanical properties	54:98	the flame retardant and mechanical properties of poly(vinyl alcohol)	54:121	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	9	19	from	mechanisms	1801:1810	arg1	phases					1837:1842	condensed and gaseous phases	1815:1842	condensed and gaseous phases	1815:1842	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	2	20	theme	facile	642:647	arg1	method					687:692	a facile ultrasonic-assisted solution blending method	640:692	a facile ultrasonic-assisted solution blending method	640:692	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	8	21	theme	excellent	1568:1576	arg1	retardancy					1584:1593	excellent flame retardancy	1568:1593	excellent flame retardancy	1568:1593	These results indicate that PEC can endow PVA with excellent flame retardancy.					
32756631	1	22	theme	mechanical	359:368	arg1	properties					370:379	deteriorating mechanical properties	345:379	deteriorating mechanical properties	345:379	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	23	dep	problems	239:246	arg1	properties					370:379	deteriorating mechanical properties	345:379	deteriorating mechanical properties	345:379	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	23	dep	problems	239:246	arg1	toxicity					395:402	potential toxicity	385:402	potential toxicity to human health and environment	385:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	23	dep	problems	239:246	arg1	compatibility					330:342	poor compatibility	325:342	poor compatibility	325:342	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	23	dep	problems	239:246	arg1	problems					239:246	some key problems	230:246	some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment	230:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	7	24	theme	sharp	1407:1411	arg1	decrease					1413:1420	a sharp decrease	1405:1420	a sharp decrease	1405:1420	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	5	25	dep	%	1109:1109	arg1	to					1102:1103	to	1102:1103	to	1102:1103	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	7	26	dep	1 	1381:1382	arg1	 8					1384:1385	 8	1384:1385	 8	1384:1385	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	10	27	contain	have	1877:1880	arg2	properties					1900:1909	better mechanical properties	1882:1909	better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC	1882:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	27	contain	have	1877:1880	arg1	composites					1866:1875	PVA/PEC composites	1858:1875	PVA/PEC composites	1858:1875	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	9	28	theme	microscopic	1613:1623	arg1	investigations					1625:1638	the microscopic investigations	1609:1638	the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1609:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	7	29	theme	release	1318:1324	arg1	pHRR					1332:1335	pHRR	1332:1335	pHRR	1332:1335	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	29	theme	release	1318:1324	arg1	rate					1326:1329	the peak heat release rate	1304:1329	the peak heat release rate (pHRR)	1304:1336	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	10	30	theme	PVA/PEC	1858:1864	arg1	composites					1866:1875	PVA/PEC composites	1858:1875	PVA/PEC composites	1858:1875	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	5	31	theme	oxygen	1045:1050	arg1	value					1064:1068	the limited oxygen index (LOI) value	1033:1068	the limited oxygen index (LOI) value of cured PVA	1033:1081	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	7	32	theme	peak	1308:1311	arg1	pHRR					1332:1335	pHRR	1332:1335	pHRR	1332:1335	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	32	theme	peak	1308:1311	arg1	rate					1326:1329	the peak heat release rate	1304:1329	the peak heat release rate (pHRR)	1304:1336	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	4	33	theme	thermal	937:943	arg1	stability					945:953	better thermal stability	930:953	better thermal stability	930:953	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	2	34	theme	solution	669:676	arg1	method					687:692	a facile ultrasonic-assisted solution blending method	640:692	a facile ultrasonic-assisted solution blending method	640:692	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	0	35	theme	retardant	64:72	arg1	properties					89:98	the flame retardant and mechanical properties	54:98	the flame retardant and mechanical properties of poly(vinyl alcohol)	54:121	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	1	36	theme	vinyl	304:308	arg1	PVA					319:321	PVA	319:321	PVA	319:321	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	36	theme	vinyl	304:308	arg1	alcohol					310:316	poly vinyl alcohol	299:316	poly vinyl alcohol (PVA)	299:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	2	37	theme	eco-friendly	472:483	arg1	complex					511:517	a green and eco-friendly bio-based polyelectrolyte complex	460:517	a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid	460:558	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	2	37	theme	eco-friendly	472:483	arg1	PEC					520:522	PEC	520:522	PEC	520:522	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	2	38	theme	retardant	594:602	arg1	properties					619:628	the flame retardant and mechanical properties	584:628	the flame retardant and mechanical properties of PVA	584:635	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	0	39	theme	mechanical	78:87	arg1	properties					89:98	the flame retardant and mechanical properties	54:98	the flame retardant and mechanical properties of poly(vinyl alcohol)	54:121	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	7	40	theme	pure	1294:1297	arg1	PVA					1299:1301	pure PVA	1294:1301	pure PVA	1294:1301	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	2	41	theme	green	462:466	arg1	complex					511:517	a green and eco-friendly bio-based polyelectrolyte complex	460:517	a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid	460:558	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	2	41	theme	green	462:466	arg1	PEC					520:522	PEC	520:522	PEC	520:522	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	10	42	theme	better	1882:1887	arg1	properties					1900:1909	better mechanical properties	1882:1909	better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC	1882:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	9	43	theme	electron	1684:1691	arg1	microscopy					1693:1702	scanning electron microscopy	1675:1702	scanning electron microscopy	1675:1702	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	5	44	theme	PVA	1079:1081	arg1	value					1064:1068	the limited oxygen index (LOI) value	1033:1068	the limited oxygen index (LOI) value of cured PVA	1033:1081	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	2	45	theme	mechanical	608:617	arg1	properties					619:628	the flame retardant and mechanical properties	584:628	the flame retardant and mechanical properties of PVA	584:635	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	3	46	from	ratio	785:789	arg1	PEC					816:818	the PEC	812:818	the PEC	812:818	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	4	47	theme	formation	964:972	arg1	ability					974:980	char formation ability	959:980	char formation ability	959:980	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	1	48	theme	retardant	276:284	arg1	treatment					286:294	the flame retardant treatment	266:294	the flame retardant treatment of poly vinyl alcohol (PVA)	266:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	7	49	theme	combustion	1474:1483	arg1	MCC					1511:1513	MCC	1511:1513	MCC	1511:1513	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	49	theme	combustion	1474:1483	arg1	measurements					1497:1508	the microscale combustion calorimeter measurements	1459:1508	the microscale combustion calorimeter measurements (MCC)	1459:1514	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	9	50	theme	Raman	1744:1748	arg1	spectroscopy					1750:1761	Raman spectroscopy	1744:1761	Raman spectroscopy	1744:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	9	51	theme	char	1643:1646	arg1	samples					1664:1670	all samples	1660:1670	all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1660:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	9	51	theme	char	1643:1646	arg1	residues					1648:1655	char residues	1643:1655	char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1643:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	9	52	dep	transform	1713:1721	arg1	infrared					1723:1730	infrared	1723:1730	transform infrared spectra	1713:1738	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	9	53	theme	possible	1776:1783	arg1	mechanisms					1801:1810	the possible flame retardant mechanisms	1772:1810	the possible flame retardant mechanisms in condensed and gaseous phases	1772:1842	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	0	54	theme	polyelectrolyte	154:168	arg1	complex					170:176	a bio-based polyelectrolyte complex	142:176	a bio-based polyelectrolyte complex formed by chitosan and phytic acid	142:211	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	5	55	theme	%	1100:1100	arg1	%					1109:1109	18% to 25.9%	1098:1109	18% to 25.9%	1098:1109	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	5	56	theme	index	1052:1056	arg1	value					1064:1068	the limited oxygen index (LOI) value	1033:1068	the limited oxygen index (LOI) value of cured PVA	1033:1081	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	2	57	theme	phytic	548:553	arg1	acid					555:558	phytic acid	548:558	phytic acid	548:558	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	6	58	dep	2 	1268:1269	arg1	 1					1271:1272	 1	1271:1272	2 : 1	1268:1272	Moreover, UL-94 V-0 rating was achieved except for the PVA/20(2 : 1) PEC.					
32756631	9	59	theme	retardant	1791:1799	arg1	mechanisms					1801:1810	the possible flame retardant mechanisms	1772:1810	the possible flame retardant mechanisms in condensed and gaseous phases	1772:1842	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	6	60	theme	V-0	1222:1224	arg1	rating					1226:1231	UL-94 V-0 rating	1216:1231	UL-94 V-0 rating	1216:1231	Moreover, UL-94 V-0 rating was achieved except for the PVA/20(2 : 1) PEC.					
32756631	1	61	theme	key	235:237	arg1	toxicity					395:402	potential toxicity	385:402	potential toxicity to human health and environment	385:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	61	theme	key	235:237	arg1	compatibility					330:342	poor compatibility	325:342	poor compatibility	325:342	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	61	theme	key	235:237	arg1	problems					239:246	some key problems	230:246	some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment	230:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	4	62	theme	Thermogravimetric	821:837	arg1	TGA					849:851	TGA	849:851	TGA	849:851	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	4	62	theme	Thermogravimetric	821:837	arg1	analysis					839:846	Thermogravimetric analysis	821:846	Thermogravimetric analysis (TGA)	821:852	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	10	63	theme	phosphonate	1969:1979	arg1	bonds					1981:1985	phosphonate bonds	1969:1985	phosphonate bonds	1969:1985	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	6	64	dep	PEC	1275:1277	arg1	2 					1268:1269	2 	1268:1269	2 : 1	1268:1272	Moreover, UL-94 V-0 rating was achieved except for the PVA/20(2 : 1) PEC.					
32756631	11	65	theme	flame	2103:2107	arg1	retardants					2109:2118	eco-friendly flame retardants	2090:2118	eco-friendly flame retardants	2090:2118	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	65	theme	flame	2103:2107	arg1	technology					2075:2084	the facile processing technology	2053:2084	the facile processing technology	2053:2084	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	65	theme	flame	2103:2107	arg1	result					2045:2050	a result	2043:2050	a result	2043:2050	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	0	66	theme	phytic	201:206	arg1	acid					208:211	phytic acid	201:211	phytic acid	201:211	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	7	67	theme	total	1346:1350	arg1	THR					1366:1368	THR	1366:1368	THR	1366:1368	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	67	theme	total	1346:1350	arg1	release					1357:1363	the total heat release	1342:1363	the total heat release (THR)	1342:1369	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	3	68	from	component	799:807	arg1	PEC					816:818	the PEC	812:818	the PEC	812:818	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	3	69	theme	mechanical	709:718	arg1	properties					740:749	the mechanical and flame retardant properties	705:749	the mechanical and flame retardant properties	705:749	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	0	70	theme	vinyl	108:112	arg1	alcohol					114:120	poly(vinyl alcohol)	103:121	poly(vinyl alcohol)	103:121	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	10	71	theme	PVA	1995:1997	arg1	movement					2015:2022	the PVA molecular chain movement	1991:2022	the PVA molecular chain movement blocked by PEC	1991:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	71	theme	PVA	1995:1997	arg1	backbones					1933:1941	their harder backbones	1920:1941	their harder backbones of chitosan	1920:1953	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	2	72	theme	bio-based	485:493	arg1	complex					511:517	a green and eco-friendly bio-based polyelectrolyte complex	460:517	a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid	460:558	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	2	72	theme	bio-based	485:493	arg1	PEC					520:522	PEC	520:522	PEC	520:522	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	2	73	theme	ultrasonic-assisted	649:667	arg1	method					687:692	a facile ultrasonic-assisted solution blending method	640:692	a facile ultrasonic-assisted solution blending method	640:692	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	1	74	theme	treatment	286:294	arg1	process					255:261	the process	251:261	the process of the flame retardant treatment of poly vinyl alcohol (PVA)	251:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	4	75	theme	PEC	895:897	arg1	introduction					879:890	the introduction	875:890	the introduction of PEC	875:897	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	8	76	theme	flame	1578:1582	arg1	retardancy					1584:1593	excellent flame retardancy	1568:1593	excellent flame retardancy	1568:1593	These results indicate that PEC can endow PVA with excellent flame retardancy.					
32756631	5	77	theme	wt	1028:1029	arg1	%					1030:1030	20 wt%	1025:1030	20 wt%	1025:1030	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	5	78	theme	LOI	1059:1061	arg1	value					1064:1068	the limited oxygen index (LOI) value	1033:1068	the limited oxygen index (LOI) value of cured PVA	1033:1081	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	11	79	theme	processing	2064:2073	arg1	retardants					2109:2118	eco-friendly flame retardants	2090:2118	eco-friendly flame retardants	2090:2118	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	79	theme	processing	2064:2073	arg1	technology					2075:2084	the facile processing technology	2053:2084	the facile processing technology	2053:2084	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	11	79	theme	processing	2064:2073	arg1	result					2045:2050	a result	2043:2050	a result	2043:2050	As a result, the facile processing technology and eco-friendly flame retardants are expected to be applied in practice.					
32756631	3	80	theme	flame	724:728	arg1	properties					740:749	the mechanical and flame retardant properties	705:749	the mechanical and flame retardant properties	705:749	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	10	81	theme	mechanical	1889:1898	arg1	properties					1900:1909	better mechanical properties	1882:1909	better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC	1882:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	7	82	theme	heat	1313:1316	arg1	pHRR					1332:1335	pHRR	1332:1335	pHRR	1332:1335	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	82	theme	heat	1313:1316	arg1	rate					1326:1329	the peak heat release rate	1304:1329	the peak heat release rate (pHRR)	1304:1336	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	10	83	theme	chain	2009:2013	arg1	movement					2015:2022	the PVA molecular chain movement	1991:2022	the PVA molecular chain movement blocked by PEC	1991:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	83	theme	chain	2009:2013	arg1	backbones					1933:1941	their harder backbones	1920:1941	their harder backbones of chitosan	1920:1953	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	0	84	theme	flame	58:62	arg1	properties					89:98	the flame retardant and mechanical properties	54:98	the flame retardant and mechanical properties of poly(vinyl alcohol)	54:121	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	1	85	theme	potential	385:393	arg1	toxicity					395:402	potential toxicity	385:402	potential toxicity to human health and environment	385:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	85	theme	potential	385:393	arg1	problems					239:246	some key problems	230:246	some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment	230:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	3	86	theme	component	799:807	arg1	ratio					785:789	the ratio	781:789	the ratio of each component in the PEC	781:818	Moreover, the mechanical and flame retardant properties could be regulated by varying the ratio of each component in the PEC.					
32756631	9	87	dep	Fourier	1705:1711	arg1	transform					1713:1721	transform	1713:1721	transform infrared spectra	1713:1738	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	2	88	theme	blending	678:685	arg1	method					687:692	a facile ultrasonic-assisted solution blending method	640:692	a facile ultrasonic-assisted solution blending method	640:692	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	1	89	theme	poly	299:302	arg1	PVA					319:321	PVA	319:321	PVA	319:321	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	89	theme	poly	299:302	arg1	alcohol					310:316	poly vinyl alcohol	299:316	poly vinyl alcohol (PVA)	299:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	7	90	theme	PEC	1388:1390	arg1	THR					1366:1368	THR	1366:1368	THR	1366:1368	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	90	theme	PEC	1388:1390	arg1	pHRR					1332:1335	pHRR	1332:1335	pHRR	1332:1335	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	90	theme	PEC	1388:1390	arg1	rate					1326:1329	the peak heat release rate	1304:1329	the peak heat release rate (pHRR)	1304:1336	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	90	theme	PEC	1388:1390	arg1	release					1357:1363	the total heat release	1342:1363	the total heat release (THR)	1342:1369	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	4	91	theme	better	930:935	arg1	stability					945:953	better thermal stability	930:953	better thermal stability	930:953	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	1	92	theme	poor	325:328	arg1	compatibility					330:342	poor compatibility	325:342	poor compatibility	325:342	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	92	theme	poor	325:328	arg1	problems					239:246	some key problems	230:246	some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment	230:434	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	1	93	theme	alcohol	310:316	arg1	treatment					286:294	the flame retardant treatment	266:294	the flame retardant treatment of poly vinyl alcohol (PVA)	266:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	9	94	theme	samples	1664:1670	arg1	samples					1664:1670	all samples	1660:1670	all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1660:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	9	94	theme	samples	1664:1670	arg1	residues					1648:1655	char residues	1643:1655	char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1643:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	2	95	theme	flame	588:592	arg1	properties					619:628	the flame retardant and mechanical properties	584:628	the flame retardant and mechanical properties of PVA	584:635	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	0	96	theme	poly	103:106	arg1	alcohol					114:120	poly(vinyl alcohol)	103:121	poly(vinyl alcohol)	103:121	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	2	97	theme	PVA	633:635	arg1	properties					619:628	the flame retardant and mechanical properties	584:628	the flame retardant and mechanical properties of PVA	584:635	To solve these issues, a green and eco-friendly bio-based polyelectrolyte complex (PEC) formed by chitosan and phytic acid was designed to enhance the flame retardant and mechanical properties of PVA by a facile ultrasonic-assisted solution blending method.					
32756631	4	98	theme	PVA/PEC	900:906	arg1	composites					908:917	PVA/PEC composites	900:917	PVA/PEC composites	900:917	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	0	99	theme	facile	2:7	arg1	strategy					19:26	A facile and green strategy	0:26	A facile and green strategy	0:26	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	5	100	theme	limited	1037:1043	arg1	value					1064:1068	the limited oxygen index (LOI) value	1033:1068	the limited oxygen index (LOI) value of cured PVA	1033:1081	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	9	101	from	investigations	1625:1638	arg1	samples					1664:1670	all samples	1660:1670	all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1660:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	9	101	from	investigations	1625:1638	arg1	residues					1648:1655	char residues	1643:1655	char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy	1643:1761	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	0	102	theme	green	13:17	arg1	strategy					19:26	A facile and green strategy	0:26	A facile and green strategy	0:26	A facile and green strategy to simultaneously enhance the flame retardant and mechanical properties of poly(vinyl alcohol) by introduction of a bio-based polyelectrolyte complex formed by chitosan and phytic acid.					
32756631	1	103	theme	flame	270:274	arg1	treatment					286:294	the flame retardant treatment	266:294	the flame retardant treatment of poly vinyl alcohol (PVA)	266:322	There are still some key problems in the process of the flame retardant treatment of poly vinyl alcohol (PVA): poor compatibility, deteriorating mechanical properties and potential toxicity to human health and environment.					
32756631	10	104	theme	harder	1926:1931	arg1	movement					2015:2022	the PVA molecular chain movement	1991:2022	the PVA molecular chain movement blocked by PEC	1991:2037	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	104	theme	harder	1926:1931	arg1	backbones					1933:1941	their harder backbones	1920:1941	their harder backbones of chitosan	1920:1953	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	10	104	theme	harder	1926:1931	arg1	formation					1956:1964	formation	1956:1964	formation of phosphonate bonds	1956:1985	In addition, PVA/PEC composites have better mechanical properties owing to their harder backbones of chitosan, formation of phosphonate bonds and the PVA molecular chain movement blocked by PEC.					
32756631	4	105	theme	char	959:962	arg1	ability					974:980	char formation ability	959:980	char formation ability	959:980	Thermogravimetric analysis (TGA) indicated that after the introduction of PEC, PVA/PEC composites maintained better thermal stability and char formation ability.					
32756631	5	106	theme	cured	1073:1077	arg1	PVA					1079:1081	cured PVA	1073:1081	cured PVA	1073:1081	Besides, when the addition of PEC reached 20 wt%, the limited oxygen index (LOI) value of cured PVA increased from 18% to 25.9%, 30.8% and 35.6% for PVA/20(2 : 1) PEC, PVA/20(1 : 2) PEC and PVA/20(1 : 8) PEC, respectively.					
32756631	9	107	theme	scanning	1675:1682	arg1	microscopy					1693:1702	scanning electron microscopy	1675:1702	scanning electron microscopy	1675:1702	Furthermore, the microscopic investigations on char residues of all samples by scanning electron microscopy, Fourier transform infrared spectra and Raman spectroscopy revealed the possible flame retardant mechanisms in condensed and gaseous phases.					
32756631	7	108	theme	calorimeter	1485:1495	arg1	MCC					1511:1513	MCC	1511:1513	MCC	1511:1513	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	108	theme	calorimeter	1485:1495	arg1	measurements					1497:1508	the microscale combustion calorimeter measurements	1459:1508	the microscale combustion calorimeter measurements (MCC)	1459:1514	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	109	theme	microscale	1463:1472	arg1	MCC					1511:1513	MCC	1511:1513	MCC	1511:1513	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	7	109	theme	microscale	1463:1472	arg1	measurements					1497:1508	the microscale combustion calorimeter measurements	1459:1508	the microscale combustion calorimeter measurements (MCC)	1459:1514	Compared with pure PVA, the peak heat release rate (pHRR) and the total heat release (THR) of PVA/20(1 : 8) PEC demonstrated a sharp decrease by 69.9% and 45.5%, respectively, in the microscale combustion calorimeter measurements (MCC).					
32756631	6	110	theme	UL-94	1216:1220	arg1	rating					1226:1231	UL-94 V-0 rating	1216:1231	UL-94 V-0 rating	1216:1231	Moreover, UL-94 V-0 rating was achieved except for the PVA/20(2 : 1) PEC.					
34860513	2	0	theme	potential	390:398	arg1	biomaterials					400:411	potential biomaterials	390:411	potential biomaterials for chronic diabetic wound healing	390:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	0	1	theme	Drug	91:94	arg1	Release					96:102	Sustained Drug Release	81:102	Sustained Drug Release	81:102	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	1	theme	Drug	91:94	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	6	2	theme	sustained	1145:1153	arg1	release					1155:1161	a longer sustained release	1136:1161	a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing	1136:1233	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	1	3	theme	amino	273:277	arg1	structures					290:299	amino acid-based structures	273:299	amino acid-based structures	273:299	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	0	4	theme	Chronic	108:114	arg1	Healing					131:137	Chronic Diabetic Wound Healing	108:137	Chronic Diabetic Wound Healing	108:137	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	4	theme	Chronic	108:114	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	8	5	theme	synthetic	1479:1487	arg1	biomaterials					1489:1500	very promising synthetic biomaterials	1464:1500	very promising synthetic biomaterials for applications in both drug delivery and wound healing	1464:1557	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	8	5	theme	synthetic	1479:1487	arg1	hydrogels					1439:1447	our developed saccharide-peptide hydrogels	1406:1447	our developed saccharide-peptide hydrogels	1406:1447	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	4	6	from	distribution	765:776	arg1	presence					816:823	the presence	812:823	the presence of HA	812:829	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	4	7	theme	uniform	757:763	arg1	distribution					765:776	uniform distribution	757:776	uniform distribution	757:776	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	4	8	theme	composite	732:740	arg1	hydrogels					742:750	composite hydrogels	732:750	composite hydrogels with uniform distribution and good mechanical properties in the presence of HA	732:829	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	1	9	theme	acid-based	279:288	arg1	structures					290:299	amino acid-based structures	273:299	amino acid-based structures	273:299	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	7	10	theme	wound	1306:1310	arg1	healing					1312:1318	chronic wound healing	1298:1318	chronic wound healing	1298:1318	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	5	11	theme	skin	997:1000	arg1	cells					1019:1023	human skin fibroblast (HSF) cells	991:1023	human skin fibroblast (HSF) cells	991:1023	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	1	12	used	used	169:172	arg2	hydrogels					148:156	Peptide hydrogels	140:156	Peptide hydrogels	140:156	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	1	13	theme	structures	290:299	arg1	terms					264:268	terms	264:268	terms of amino acid-based structures and functions	264:313	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	0	14	theme	Wound	125:129	arg1	Healing					131:137	Chronic Diabetic Wound Healing	108:137	Chronic Diabetic Wound Healing	108:137	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	14	theme	Wound	125:129	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	5	15	theme	injection	927:935	arg1	operation					952:960	injection syringe needle operation	927:960	injection syringe needle operation	927:960	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	6	16	theme	nanofibers	1060:1069	arg1	structure					1039:1047	the structure	1035:1047	the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel	1035:1122	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	3	17	theme	different	554:562	arg1	moieties					573:580	different aromatic moieties	554:580	different aromatic moieties	554:580	Herein, hyaluronic acid (HA) was incorporated into diphenylalanine (FF) conjugated with different aromatic moieties by a one-pot reaction.					
34860513	5	18	theme	needle	945:950	arg1	operation					952:960	injection syringe needle operation	927:960	injection syringe needle operation	927:960	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	1	19	theme	biomedical	178:187	arg1	applications					189:200	biomedical applications	178:200	biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions	178:313	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	0	20	theme	Diabetic	116:123	arg1	Healing					131:137	Chronic Diabetic Wound Healing	108:137	Chronic Diabetic Wound Healing	108:137	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	20	theme	Diabetic	116:123	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	7	21	theme	diabetic	1357:1364	arg1	model					1372:1376	the streptozotocin-induced type I diabetic mouse model	1323:1376	the streptozotocin-induced type I diabetic mouse model	1323:1376	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	3	22	theme	aromatic	564:571	arg1	moieties					573:580	different aromatic moieties	554:580	different aromatic moieties	554:580	Herein, hyaluronic acid (HA) was incorporated into diphenylalanine (FF) conjugated with different aromatic moieties by a one-pot reaction.					
34860513	6	23	theme	composite	1105:1113	arg1	hydrogel					1115:1122	the composite hydrogel	1101:1122	the composite hydrogel	1101:1122	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	2	24	theme	wound	434:438	arg1	healing					440:446	chronic diabetic wound healing	417:446	chronic diabetic wound healing	417:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	7	25	theme	curcumin-loaded	1240:1254	arg1	hydrogels					1274:1282	The curcumin-loaded N-FF/HA composite hydrogels	1236:1282	The curcumin-loaded N-FF/HA composite hydrogels	1236:1282	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	7	26	theme	streptozotocin-induced	1327:1348	arg1	type					1350:1353	the streptozotocin-induced type I	1323:1355	the streptozotocin-induced type I diabetic mouse model	1323:1376	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	5	27	from	operation	952:960	arg1	cells					1019:1023	human skin fibroblast (HSF) cells	991:1023	human skin fibroblast (HSF) cells	991:1023	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	8	28	theme	saccharide-peptide	1420:1437	arg1	biomaterials					1489:1500	very promising synthetic biomaterials	1464:1500	very promising synthetic biomaterials for applications in both drug delivery and wound healing	1464:1557	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	8	28	theme	saccharide-peptide	1420:1437	arg1	hydrogels					1439:1447	our developed saccharide-peptide hydrogels	1406:1447	our developed saccharide-peptide hydrogels	1406:1447	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	6	29	theme	networks	1085:1092	arg1	structure					1039:1047	the structure	1035:1047	the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel	1035:1122	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	2	30	theme	diabetic	425:432	arg1	healing					440:446	chronic diabetic wound healing	417:446	chronic diabetic wound healing	417:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	0	31	theme	Ultrashort	0:9	arg1	Healing					131:137	Chronic Diabetic Wound Healing	108:137	Chronic Diabetic Wound Healing	108:137	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	31	theme	Ultrashort	0:9	arg1	Release					96:102	Sustained Drug Release	81:102	Sustained Drug Release	81:102	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	31	theme	Ultrashort	0:9	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	6	32	theme	wound	1221:1225	arg1	healing					1227:1233	wound healing	1221:1233	wound healing	1221:1233	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	5	33	theme	composite	853:861	arg1	hydrogel					863:870	The obtained N-FF/HA composite hydrogel	832:870	The obtained N-FF/HA composite hydrogel	832:870	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	5	34	theme	good	966:969	arg1	biocompatibility					971:986	good biocompatibility	966:986	good biocompatibility	966:986	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	8	35	from	applications	1506:1517	arg1	delivery					1532:1539	drug delivery	1527:1539	drug delivery	1527:1539	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	8	35	from	applications	1506:1517	arg1	healing					1551:1557	wound healing	1545:1557	wound healing	1545:1557	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	2	36	theme	chronic	417:423	arg1	healing					440:446	chronic diabetic wound healing	417:446	chronic diabetic wound healing	417:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	0	37	theme	Hyaluronic	24:33	arg1	Hydrogels					67:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	1	38	theme	functions	305:313	arg1	terms					264:268	terms	264:268	terms of amino acid-based structures and functions	264:313	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	8	39	theme	drug	1527:1530	arg1	delivery					1532:1539	drug delivery	1527:1539	drug delivery	1527:1539	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	6	40	theme	curcumin	1166:1173	arg1	release					1155:1161	a longer sustained release	1136:1161	a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing	1136:1233	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	5	41	theme	human	991:995	arg1	cells					1019:1023	human skin fibroblast (HSF) cells	991:1023	human skin fibroblast (HSF) cells	991:1023	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	8	42	theme	developed	1410:1418	arg1	biomaterials					1489:1500	very promising synthetic biomaterials	1464:1500	very promising synthetic biomaterials for applications in both drug delivery and wound healing	1464:1557	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	8	42	theme	developed	1410:1418	arg1	hydrogels					1439:1447	our developed saccharide-peptide hydrogels	1406:1447	our developed saccharide-peptide hydrogels	1406:1447	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	5	43	theme	fibroblast	1002:1011	arg1	cells					1019:1023	human skin fibroblast (HSF) cells	991:1023	human skin fibroblast (HSF) cells	991:1023	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	3	44	theme	one-pot	587:593	arg1	reaction					595:602	a one-pot reaction	585:602	a one-pot reaction	585:602	Herein, hyaluronic acid (HA) was incorporated into diphenylalanine (FF) conjugated with different aromatic moieties by a one-pot reaction.					
34860513	1	45	theme	good	217:220	arg1	biocompatibility					222:237	their good biocompatibility	211:237	their good biocompatibility	211:237	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	0	46	theme	Injectable	46:55	arg1	Hydrogels					67:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	5	47	theme	syringe	937:943	arg1	operation					952:960	injection syringe needle operation	927:960	injection syringe needle operation	927:960	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	6	48	theme	thinner	1052:1058	arg1	nanofibers					1060:1069	thinner nanofibers	1052:1069	thinner nanofibers	1052:1069	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	6	49	theme	honeycomb	1075:1083	arg1	networks					1085:1092	honeycomb networks	1075:1092	honeycomb networks	1075:1092	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	5	50	theme	obtained	836:843	arg1	hydrogel					863:870	The obtained N-FF/HA composite hydrogel	832:870	The obtained N-FF/HA composite hydrogel	832:870	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	0	51	theme	Acid-Based	35:44	arg1	Hydrogels					67:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	4	52	with	hydrogels	742:750	arg1	properties					798:807	good mechanical properties	782:807	good mechanical properties	782:807	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	4	52	with	hydrogels	742:750	arg1	distribution					765:776	uniform distribution	757:776	uniform distribution	757:776	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	5	53	theme	N-FF/HA	845:851	arg1	hydrogel					863:870	The obtained N-FF/HA composite hydrogel	832:870	The obtained N-FF/HA composite hydrogel	832:870	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	3	54	theme	hyaluronic	474:483	arg1	HA					491:492	HA	491:492	HA	491:492	Herein, hyaluronic acid (HA) was incorporated into diphenylalanine (FF) conjugated with different aromatic moieties by a one-pot reaction.					
34860513	3	54	theme	hyaluronic	474:483	arg1	acid					485:488	hyaluronic acid	474:488	hyaluronic acid (HA)	474:493	Herein, hyaluronic acid (HA) was incorporated into diphenylalanine (FF) conjugated with different aromatic moieties by a one-pot reaction.					
34860513	4	55	theme	HA	828:829	arg1	presence					816:823	the presence	812:823	the presence of HA	812:829	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	2	56	theme	hydrogels	377:385	arg1	exploration					329:339	the exploration	325:339	the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing	325:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	0	57	theme	Composite	57:65	arg1	Hydrogels					67:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Hyaluronic Acid-Based Injectable Composite Hydrogels	24:75	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	8	58	theme	promising	1469:1477	arg1	biomaterials					1489:1500	very promising synthetic biomaterials	1464:1500	very promising synthetic biomaterials for applications in both drug delivery and wound healing	1464:1557	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	8	58	theme	promising	1469:1477	arg1	hydrogels					1439:1447	our developed saccharide-peptide hydrogels	1406:1447	our developed saccharide-peptide hydrogels	1406:1447	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	7	59	theme	N-FF/HA	1256:1262	arg1	hydrogels					1274:1282	The curcumin-loaded N-FF/HA composite hydrogels	1236:1282	The curcumin-loaded N-FF/HA composite hydrogels	1236:1282	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	8	60	theme	wound	1545:1549	arg1	healing					1551:1557	wound healing	1545:1557	wound healing	1545:1557	The results suggested that our developed saccharide-peptide hydrogels could serve as very promising synthetic biomaterials for applications in both drug delivery and wound healing in the future.					
34860513	2	61	theme	composite	367:375	arg1	hydrogels					377:385	the peptide/saccharide composite hydrogels	344:385	the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing	344:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	5	62	from	biocompatibility	971:986	arg1	cells					1019:1023	human skin fibroblast (HSF) cells	991:1023	human skin fibroblast (HSF) cells	991:1023	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	7	63	theme	chronic	1298:1304	arg1	healing					1312:1318	chronic wound healing	1298:1318	chronic wound healing	1298:1318	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	5	64	theme	HSF	1014:1016	arg1	cells					1019:1023	human skin fibroblast (HSF) cells	991:1023	human skin fibroblast (HSF) cells	991:1023	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	4	65	theme	mechanical	787:796	arg1	properties					798:807	good mechanical properties	782:807	good mechanical properties	782:807	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	1	66	theme	Peptide	140:146	arg1	hydrogels					148:156	Peptide hydrogels	140:156	Peptide hydrogels	140:156	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	1	67	theme	unique	243:248	arg1	advantages					250:259	unique advantages	243:259	unique advantages	243:259	Peptide hydrogels are widely used for biomedical applications owing to their good biocompatibility and unique advantages in terms of amino acid-based structures and functions.					
34860513	6	68	theme	hydrophobic	1178:1188	arg1	drug					1190:1193	a hydrophobic drug	1176:1193	a hydrophobic drug for anti-inflammation and wound healing	1176:1233	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	6	68	theme	hydrophobic	1178:1188	arg1	curcumin					1166:1173	curcumin	1166:1173	curcumin	1166:1173	Besides, the structure of thinner nanofibers and honeycomb networks inside the composite hydrogel allowed for a longer sustained release of curcumin, a hydrophobic drug for anti-inflammation and wound healing.					
34860513	4	69	theme	good	782:785	arg1	properties					798:807	good mechanical properties	782:807	good mechanical properties	782:807	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	5	70	theme	self-healing	899:910	arg1	properties					912:921	greatly improved self-healing properties	882:921	greatly improved self-healing properties	882:921	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	7	71	theme	mouse	1366:1370	arg1	model					1372:1376	the streptozotocin-induced type I diabetic mouse model	1323:1376	the streptozotocin-induced type I diabetic mouse model	1323:1376	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	2	72	theme	peptide/saccharide	348:365	arg1	hydrogels					377:385	the peptide/saccharide composite hydrogels	344:385	the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing	344:446	However, the exploration of the peptide/saccharide composite hydrogels as potential biomaterials for chronic diabetic wound healing is still limited.					
34860513	0	73	theme	Sustained	81:89	arg1	Release					96:102	Sustained Drug Release	81:102	Sustained Drug Release	81:102	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	73	theme	Sustained	81:89	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	7	74	theme	type	1350:1353	arg1	model					1372:1376	the streptozotocin-induced type I diabetic mouse model	1323:1376	the streptozotocin-induced type I diabetic mouse model	1323:1376	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	0	75	dep	Peptides	11:18	arg1	Healing					131:137	Chronic Diabetic Wound Healing	108:137	Chronic Diabetic Wound Healing	108:137	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	75	dep	Peptides	11:18	arg1	Release					96:102	Sustained Drug Release	81:102	Sustained Drug Release	81:102	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	0	75	dep	Peptides	11:18	arg1	Peptides					11:18	Ultrashort Peptides	0:18	Ultrashort Peptides	0:18	Ultrashort Peptides and Hyaluronic Acid-Based Injectable Composite Hydrogels for Sustained Drug Release and Chronic Diabetic Wound Healing.					
34860513	4	76	from	properties	798:807	arg1	presence					816:823	the presence	812:823	the presence of HA	812:829	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34860513	5	77	theme	improved	890:897	arg1	properties					912:921	greatly improved self-healing properties	882:921	greatly improved self-healing properties	882:921	The obtained N-FF/HA composite hydrogel exhibited greatly improved self-healing properties via injection syringe needle operation and good biocompatibility on human skin fibroblast (HSF) cells.					
34860513	7	78	theme	composite	1264:1272	arg1	hydrogels					1274:1282	The curcumin-loaded N-FF/HA composite hydrogels	1236:1282	The curcumin-loaded N-FF/HA composite hydrogels	1236:1282	The curcumin-loaded N-FF/HA composite hydrogels could promote chronic wound healing in the streptozotocin-induced type I diabetic mouse model.					
34860513	4	79	theme	dipeptide	633:641	arg1	derivatives					643:653	the dipeptide derivatives	629:653	the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P)	629:710	Our results showed that the dipeptide derivatives modified by benzene (B), naphthalene (N), and pyrene (P) self-assembled into composite hydrogels with uniform distribution and good mechanical properties in the presence of HA.					
34420538	0	0	theme	ingestion	136:144	arg1	form					106:109	their form	100:109	their form (solid and/or liquid) of ingestion	100:144	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	3	1	theme	paralleled	429:438	arg1	experiments					440:450	two paralleled experiments	425:450	two paralleled experiments (aspartame and sucralose)	425:476	Thus, two paralleled experiments (aspartame and sucralose) were conducted.					
34420538	3	1	theme	paralleled	429:438	arg1	sucralose					467:475	sucralose	467:475	sucralose	467:475	Thus, two paralleled experiments (aspartame and sucralose) were conducted.					
34420538	3	1	theme	paralleled	429:438	arg1	aspartame					453:461	aspartame	453:461	aspartame	453:461	Thus, two paralleled experiments (aspartame and sucralose) were conducted.					
34420538	0	2	from	effect	4:9	arg1	measures					60:67	body weight measures	48:67	body weight measures	48:67	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	0	2	from	effect	4:9	arg1	metabolites					79:89	blood metabolites	73:89	blood metabolites	73:89	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	2	3	theme	inconclusive	302:313	arg1	relation					315:322	the inconclusive relation	298:322	the inconclusive relation between NCS and body weight	298:350	However, the inconclusive relation between NCS and body weight may partially relate to their form of ingestion (solid or liquid).					
34420538	7	4	contain	had	923:925	arg1	rats					918:921	All rats	914:921	All rats	914:921	All rats had free access to food and water for 7 weeks.					
34420538	7	4	contain	had	923:925	arg2	access					932:937	free access	927:937	free access to food and water for 7 weeks	927:967	All rats had free access to food and water for 7 weeks.					
34420538	5	5	theme	plain	751:755	arg1	water					757:761	plain water	751:761	plain water	751:761	In Expt 1, aspartame (0·05 %) was added to the diet (AD) or drinking water (AW) or both diet and water (ADW), and a control group (C) was given a non-sweetened diet with plain water.					
34420538	12	6	theme	Sucralose	1356:1364	arg1	ingestion					1366:1374	Sucralose ingestion	1356:1374	Sucralose ingestion	1356:1374	Sucralose ingestion had a similar impact to that of aspartame though to a lesser extent.					
34420538	0	7	theme	blood	73:77	arg1	metabolites					79:89	blood metabolites	73:89	blood metabolites	73:89	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	12	8	theme	lesser	1430:1435	arg1	extent					1437:1442	a lesser extent	1428:1442	a lesser extent	1428:1442	Sucralose ingestion had a similar impact to that of aspartame though to a lesser extent.					
34420538	8	9	theme	body	985:988	arg1	weight					990:995	body weight	985:995	body weight	985:995	Energy intake, body weight and body composition were monitored and blood metabolites were determined.					
34420538	13	10	theme	adverse	1508:1514	arg1	effects					1516:1522	adverse effects	1508:1522	adverse effects	1508:1522	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	4	11	theme	Dawley	511:516	arg1	rats					518:521	Sprague Dawley rats	503:521	Sprague Dawley rats (7-week-old male)	503:539	In each, Sprague Dawley rats (7-week-old male) were randomly divided into four groups.					
34420538	4	11	theme	Dawley	511:516	arg1	male					535:538	7-week-old male	524:538	7-week-old male	524:538	In each, Sprague Dawley rats (7-week-old male) were randomly divided into four groups.					
34420538	11	12	theme	glucose	1335:1341	arg1	intolerance					1343:1353	glucose intolerance	1335:1353	glucose intolerance	1335:1353	Additionally, aspartame ingestion was associated with glucose intolerance.					
34420538	9	13	from	increase	1178:1185	arg1	efficiency					1197:1206	energy efficiency	1190:1206	energy efficiency	1190:1206	Results showed that aspartame ingestion significantly increased body weight and fat mass mainly due to an increase in energy efficiency.					
34420538	8	14	theme	body	1001:1004	arg1	composition					1006:1016	body composition	1001:1016	body composition	1001:1016	Energy intake, body weight and body composition were monitored and blood metabolites were determined.					
34420538	9	15	theme	aspartame	1092:1100	arg1	ingestion					1102:1110	aspartame ingestion	1092:1110	aspartame ingestion	1092:1110	Results showed that aspartame ingestion significantly increased body weight and fat mass mainly due to an increase in energy efficiency.					
34420538	13	16	theme	ingestion	1578:1586	arg1	form					1570:1573	the form	1566:1573	the form of ingestion	1566:1586	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	13	17	theme	body	1527:1530	arg1	measures					1532:1539	body measures	1527:1539	body measures that were not related to the form of ingestion	1527:1586	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	2	18	theme	body	340:343	arg1	weight					345:350	body weight	340:350	body weight	340:350	However, the inconclusive relation between NCS and body weight may partially relate to their form of ingestion (solid or liquid).					
34420538	2	19	theme	ingestion	390:398	arg1	form					382:385	their form	376:385	their form of ingestion (solid or liquid)	376:416	However, the inconclusive relation between NCS and body weight may partially relate to their form of ingestion (solid or liquid).					
34420538	2	20	dep	ingestion	390:398	arg1	liquid					410:415	liquid	410:415	liquid	410:415	However, the inconclusive relation between NCS and body weight may partially relate to their form of ingestion (solid or liquid).					
34420538	2	20	dep	ingestion	390:398	arg1	solid					401:405	solid	401:405	solid	401:405	However, the inconclusive relation between NCS and body weight may partially relate to their form of ingestion (solid or liquid).					
34420538	3	21	dep	experiments	440:450	arg1	experiments					440:450	two paralleled experiments	425:450	two paralleled experiments (aspartame and sucralose)	425:476	Thus, two paralleled experiments (aspartame and sucralose) were conducted.					
34420538	3	21	dep	experiments	440:450	arg1	sucralose					467:475	sucralose	467:475	sucralose	467:475	Thus, two paralleled experiments (aspartame and sucralose) were conducted.					
34420538	3	21	dep	experiments	440:450	arg1	aspartame					453:461	aspartame	453:461	aspartame	453:461	Thus, two paralleled experiments (aspartame and sucralose) were conducted.					
34420538	13	22	theme	aspartame	1480:1488	arg1	ingestion					1467:1475	7-week ingestion	1460:1475	7-week ingestion of aspartame and sucralose	1460:1502	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	0	23	theme	aspartame	14:22	arg1	effect					4:9	The effect	0:9	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.	0:145	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	1	24	from	drink	210:214	arg1	NCS					188:190	NCS	188:190	NCS	188:190	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	24	from	drink	210:214	arg1	ingestion					151:159	The ingestion	147:159	The ingestion of non-caloric sweeteners (NCS) from food and/or drink	147:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	24	from	drink	210:214	arg1	sweeteners					176:185	non-caloric sweeteners	164:185	non-caloric sweeteners (NCS) from food and/or drink	164:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	9	25	theme	body	1136:1139	arg1	weight					1141:1146	body weight	1136:1146	body weight	1136:1146	Results showed that aspartame ingestion significantly increased body weight and fat mass mainly due to an increase in energy efficiency.					
34420538	5	26	theme	non-sweetened	727:739	arg1	diet					741:744	a non-sweetened diet	725:744	a non-sweetened diet	725:744	In Expt 1, aspartame (0·05 %) was added to the diet (AD) or drinking water (AW) or both diet and water (ADW), and a control group (C) was given a non-sweetened diet with plain water.					
34420538	9	27	theme	energy	1190:1195	arg1	efficiency					1197:1206	energy efficiency	1190:1206	energy efficiency	1190:1206	Results showed that aspartame ingestion significantly increased body weight and fat mass mainly due to an increase in energy efficiency.					
34420538	13	28	theme	sucralose	1494:1502	arg1	ingestion					1467:1475	7-week ingestion	1460:1475	7-week ingestion of aspartame and sucralose	1460:1502	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	0	29	theme	sucralose	28:36	arg1	effect					4:9	The effect	0:9	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.	0:145	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	1	30	theme	caloric	239:245	arg1	intake					247:252	caloric intake	239:252	caloric intake	239:252	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	31	from	food	198:201	arg1	NCS					188:190	NCS	188:190	NCS	188:190	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	31	from	food	198:201	arg1	ingestion					151:159	The ingestion	147:159	The ingestion of non-caloric sweeteners (NCS) from food and/or drink	147:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	31	from	food	198:201	arg1	sweeteners					176:185	non-caloric sweeteners	164:185	non-caloric sweeteners (NCS) from food and/or drink	164:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	32	theme	non-caloric	164:174	arg1	NCS					188:190	NCS	188:190	NCS	188:190	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	32	theme	non-caloric	164:174	arg1	sweeteners					176:185	non-caloric sweeteners	164:185	non-caloric sweeteners (NCS) from food and/or drink	164:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	5	33	theme	drinking	641:648	arg1	water					650:654	drinking water	641:654	drinking water (AW)	641:659	In Expt 1, aspartame (0·05 %) was added to the diet (AD) or drinking water (AW) or both diet and water (ADW), and a control group (C) was given a non-sweetened diet with plain water.					
34420538	5	33	theme	drinking	641:648	arg1	AW					657:658	AW	657:658	AW	657:658	In Expt 1, aspartame (0·05 %) was added to the diet (AD) or drinking water (AW) or both diet and water (ADW), and a control group (C) was given a non-sweetened diet with plain water.					
34420538	0	34	theme	form	106:109	arg1	role					92:95	role	92:95	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.	0:145	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	9	35	theme	fat	1152:1154	arg1	mass					1156:1159	fat mass	1152:1159	fat mass	1152:1159	Results showed that aspartame ingestion significantly increased body weight and fat mass mainly due to an increase in energy efficiency.					
34420538	8	36	theme	blood	1037:1041	arg1	metabolites					1043:1053	blood metabolites	1037:1053	blood metabolites	1037:1053	Energy intake, body weight and body composition were monitored and blood metabolites were determined.					
34420538	1	37	theme	sweeteners	176:185	arg1	ingestion					151:159	The ingestion	147:159	The ingestion of non-caloric sweeteners (NCS) from food and/or drink	147:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	13	38	theme	7-week	1460:1465	arg1	ingestion					1467:1475	7-week ingestion	1460:1475	7-week ingestion of aspartame and sucralose	1460:1502	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	13	39	contain	had	1504:1506	arg2	effects					1516:1522	adverse effects	1508:1522	adverse effects	1508:1522	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	13	39	contain	had	1504:1506	arg1	ingestion					1467:1475	7-week ingestion	1460:1475	7-week ingestion of aspartame and sucralose	1460:1502	In conclusion, 7-week ingestion of aspartame and sucralose had adverse effects on body measures that were not related to the form of ingestion.					
34420538	1	40	from	ingestion	151:159	arg1	drink					210:214	drink	210:214	drink	210:214	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	1	40	from	ingestion	151:159	arg1	food					198:201	food	198:201	food	198:201	The ingestion of non-caloric sweeteners (NCS) from food and/or drink was intended to reduce caloric intake without compromising palatability.					
34420538	7	41	theme	free	927:930	arg1	access					932:937	free access	927:937	free access to food and water for 7 weeks	927:967	All rats had free access to food and water for 7 weeks.					
34420538	0	42	dep	aspartame	14:22	arg1	intake					38:43	intake	38:43	intake	38:43	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	4	43	theme	7-week-old	524:533	arg1	rats					518:521	Sprague Dawley rats	503:521	Sprague Dawley rats (7-week-old male)	503:539	In each, Sprague Dawley rats (7-week-old male) were randomly divided into four groups.					
34420538	4	43	theme	7-week-old	524:533	arg1	male					535:538	7-week-old male	524:538	7-week-old male	524:538	In each, Sprague Dawley rats (7-week-old male) were randomly divided into four groups.					
34420538	12	44	contain	had	1376:1378	arg2	impact					1390:1395	a similar impact	1380:1395	a similar impact	1380:1395	Sucralose ingestion had a similar impact to that of aspartame though to a lesser extent.					
34420538	12	44	contain	had	1376:1378	arg1	ingestion					1366:1374	Sucralose ingestion	1356:1374	Sucralose ingestion	1356:1374	Sucralose ingestion had a similar impact to that of aspartame though to a lesser extent.					
34420538	6	45	theme	control	895:901	arg1	C					910:910	C	910:910	C	910:910	In Expt 2, sucralose (0·016 %) was similarly provided in the diet (SD) or drinking water (SW) or both diet and water (SDW), with a control group (C).					
34420538	6	45	theme	control	895:901	arg1	group					903:907	a control group	893:907	a control group (C)	893:911	In Expt 2, sucralose (0·016 %) was similarly provided in the diet (SD) or drinking water (SW) or both diet and water (SDW), with a control group (C).					
34420538	5	46	theme	control	697:703	arg1	group					705:709	a control group	695:709	a control group (C)	695:713	In Expt 1, aspartame (0·05 %) was added to the diet (AD) or drinking water (AW) or both diet and water (ADW), and a control group (C) was given a non-sweetened diet with plain water.					
34420538	5	46	theme	control	697:703	arg1	C					712:712	C	712:712	C	712:712	In Expt 1, aspartame (0·05 %) was added to the diet (AD) or drinking water (AW) or both diet and water (ADW), and a control group (C) was given a non-sweetened diet with plain water.					
34420538	0	47	theme	weight	53:58	arg1	measures					60:67	body weight measures	48:67	body weight measures	48:67	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	6	48	theme	drinking	838:845	arg1	SW					854:855	SW	854:855	SW	854:855	In Expt 2, sucralose (0·016 %) was similarly provided in the diet (SD) or drinking water (SW) or both diet and water (SDW), with a control group (C).					
34420538	6	48	theme	drinking	838:845	arg1	water					847:851	drinking water	838:851	drinking water (SW)	838:856	In Expt 2, sucralose (0·016 %) was similarly provided in the diet (SD) or drinking water (SW) or both diet and water (SDW), with a control group (C).					
34420538	0	49	dep	effect	4:9	arg1	role					92:95	role	92:95	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.	0:145	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	8	50	theme	Energy	970:975	arg1	intake					977:982	Energy intake	970:982	Energy intake	970:982	Energy intake, body weight and body composition were monitored and blood metabolites were determined.					
34420538	12	51	theme	similar	1382:1388	arg1	impact					1390:1395	a similar impact	1380:1395	a similar impact	1380:1395	Sucralose ingestion had a similar impact to that of aspartame though to a lesser extent.					
34420538	0	52	theme	body	48:51	arg1	measures					60:67	body weight measures	48:67	body weight measures	48:67	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	11	53	theme	aspartame	1295:1303	arg1	ingestion					1305:1313	aspartame ingestion	1295:1313	aspartame ingestion	1295:1313	Additionally, aspartame ingestion was associated with glucose intolerance.					
34420538	0	54	dep	form	106:109	arg1	liquid					125:130	liquid	125:130	liquid	125:130	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	0	54	dep	form	106:109	arg1	solid					112:116	solid	112:116	solid	112:116	The effect of aspartame and sucralose intake on body weight measures and blood metabolites: role of their form (solid and/or liquid) of ingestion.					
34420538	10	55	theme	ingestion	1270:1278	arg1	form					1262:1265	the form	1258:1265	the amount rather than the form of ingestion	1235:1278	The effect was related to the amount rather than the form of ingestion.					
32275990	4	0	theme	6S-GalNAc	1025:1033	arg1	sulfation					1035:1043	prevalent 6S-GalNAc sulfation	1015:1043	prevalent 6S-GalNAc sulfation (68%)	1015:1049	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	0	theme	6S-GalNAc	1025:1033	arg1	%					1048:1048	68%	1046:1048	68%	1046:1048	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	3	1	theme	ultrafiltration	692:706	arg1	membrane					708:715	ultrafiltration membrane	692:715	ultrafiltration membrane for CS production	692:733	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	3	2	theme	this	483:486	arg1	result					473:478	result	473:478	result of this	473:486	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	3	2	theme	this	483:486	arg1	process					502:508	an optimized process	489:508	an optimized process in sequential steps for the recovery of both biomaterials and their purification process	489:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	5	3	theme	purified	1207:1214	arg1	whitlockite/β-TCP					1275:1291	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	7	4	theme	fraction	1527:1534	arg1	composition					1500:1510	the composition	1496:1510	the composition of the mineral fraction	1496:1534	This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction.					
32275990	4	5	theme	Magnetic	884:891	arg1	Resonance					893:901	Nuclear Magnetic Resonance	876:901	Nuclear Magnetic Resonance	876:901	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	5	6	theme	biphasic	1216:1223	arg1	phosphate					1246:1254	biphasic 60:40 porous calcium phosphate	1216:1254	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	5	7	theme	porous	1231:1236	arg1	phosphate					1246:1254	biphasic 60:40 porous calcium phosphate	1216:1254	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	4	8	with	sulfation	1035:1043	arg1	weight					993:998	a molecular weight	981:998	a molecular weight of 67 kDa	981:1008	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	9	theme	Strong	907:912	arg1	Chromatography					929:942	Strong Anion Exchange Chromatography	907:942	Strong Anion Exchange Chromatography	907:942	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	2	10	theme	cross	281:285	arg1	section					287:293	cross section	281:293	cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	281:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	5	11	theme	60:40	1225:1229	arg1	phosphate					1246:1254	biphasic 60:40 porous calcium phosphate	1216:1254	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	0	12	theme	structural	91:100	arg1	characterization					102:117	structural characterization	91:117	structural characterization	91:117	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	3	13	theme	CS	721:722	arg1	production					724:733	CS production	721:733	CS production	721:733	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	5	14	theme	bioapatite	1194:1203	arg1	case					1182:1185	the case	1178:1185	the case of the bioapatite	1178:1203	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	disaccharides					1120:1132	disulfated disaccharides	1109:1132	disulfated disaccharides	1109:1132	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	4S-GalNAc					1064:1072	4S-GalNAc	1064:1072	4S-GalNAc (13%)	1064:1078	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	%					1150:1150	only 7%	1144:1150	only 7% of non-sulfated units	1144:1172	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	proportion					1095:1104	a significant proportion	1081:1104	a significant proportion of disulfated disaccharides (12%)	1081:1138	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	%					1137:1137	12%	1135:1137	12%	1135:1137	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	%					1077:1077	13%	1075:1077	13%	1075:1077	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	15	theme	disaccharides	1120:1132	arg1	units					1168:1172	non-sulfated units	1155:1172	non-sulfated units	1155:1172	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	7	16	theme	mineral	1519:1525	arg1	fraction					1527:1534	the mineral fraction	1515:1534	the mineral fraction	1515:1534	This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction.					
32275990	4	17	with	material	967:974	arg1	weight					993:998	a molecular weight	981:998	a molecular weight of 67 kDa	981:1008	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	18	theme	disulfated	1109:1118	arg1	disaccharides					1120:1132	disulfated disaccharides	1109:1132	disulfated disaccharides	1109:1132	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	1	19	theme	Prionace	162:169	arg1	jaw					178:180	the Prionace glauca jaw	158:180	the Prionace glauca jaw	158:180	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
32275990	4	20	theme	molecular	983:991	arg1	weight					993:998	a molecular weight	981:998	a molecular weight of 67 kDa	981:1008	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	21	theme	Permeation	849:858	arg1	Chromatography					860:873	Gel Permeation Chromatography	845:873	Gel Permeation Chromatography	845:873	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	2	22	from	prevalence	337:346	arg1	mineralized					369:379	mineralized	369:379	mineralized	369:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	2	22	from	prevalence	337:346	arg1	tissue					359:364	organic tissue	351:364	organic tissue	351:364	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	2	23	theme	sandwich-type	254:266	arg1	structure					268:276	The sandwich-type structure	250:276	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	250:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	2	23	theme	sandwich-type	254:266	arg1	zones					463:467	CS or hydroxyapatite -enriched zones	432:467	CS or hydroxyapatite -enriched zones	432:467	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	1	24	theme	glauca	171:176	arg1	jaw					178:180	the Prionace glauca jaw	158:180	the Prionace glauca jaw	158:180	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
32275990	2	25	theme	CS	432:433	arg1	structure					268:276	The sandwich-type structure	250:276	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	250:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	2	25	theme	CS	432:433	arg1	zones					463:467	CS or hydroxyapatite -enriched zones	432:467	CS or hydroxyapatite -enriched zones	432:467	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	4	26	theme	Nuclear	876:882	arg1	Resonance					893:901	Nuclear Magnetic Resonance	876:901	Nuclear Magnetic Resonance	876:901	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	disaccharides					1120:1132	disulfated disaccharides	1109:1132	disulfated disaccharides	1109:1132	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	4S-GalNAc					1064:1072	4S-GalNAc	1064:1072	4S-GalNAc (13%)	1064:1078	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	%					1150:1150	only 7%	1144:1150	only 7% of non-sulfated units	1144:1172	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	proportion					1095:1104	a significant proportion	1081:1104	a significant proportion of disulfated disaccharides (12%)	1081:1138	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	%					1137:1137	12%	1135:1137	12%	1135:1137	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	%					1077:1077	13%	1075:1077	13%	1075:1077	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	27	theme	units	1168:1172	arg1	units					1168:1172	non-sulfated units	1155:1172	non-sulfated units	1155:1172	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	2	28	theme	jaw	302:304	arg1	section					287:293	cross section	281:293	cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	281:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	1	29	theme	jaw	178:180	arg1	potential					145:153	the potential	141:153	the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite	141:235	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
32275990	0	30	theme	Chondroitin	0:10	arg1	sulfate					12:18	Chondroitin sulfate	0:18	Chondroitin sulfate	0:18	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	3	31	theme	controlled	749:758	arg1	treatment					768:776	controlled thermal treatment	749:776	controlled thermal treatment for hydroxyapatite obtaining	749:805	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	4	32	theme	significant	1083:1093	arg1	proportion					1095:1104	a significant proportion	1081:1104	a significant proportion of disulfated disaccharides (12%)	1081:1138	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	32	theme	significant	1083:1093	arg1	%					1137:1137	12%	1135:1137	12%	1135:1137	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	3	33	theme	purification	578:589	arg1	process					591:597	their purification process	572:597	their purification process	572:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	6	34	theme	thermal	1401:1407	arg1	treatment					1409:1417	the thermal treatment	1397:1417	the thermal treatment	1397:1417	Hydroxyapatite as major component (85%) was also obtained for jaws directly subjected to the thermal treatment.					
32275990	5	35	theme	hydroxyapatite	1259:1272	arg1	phosphate					1246:1254	biphasic 60:40 porous calcium phosphate	1216:1254	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	0	36	theme	Prionace	44:51	arg1	jaw					66:68	Prionace glauca shark jaw	44:68	Prionace glauca shark jaw	44:68	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	2	37	theme	alternate	315:323	arg1	layers					325:330	alternate layers	315:330	alternate layers with prevalence in organic tissue or mineralized	315:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	3	38	theme	enzymatic	625:633	arg1	proteolysis					635:645	enzymatic proteolysis	625:645	enzymatic proteolysis	625:645	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	2	39	theme	-enriched	453:461	arg1	structure					268:276	The sandwich-type structure	250:276	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	250:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	2	39	theme	-enriched	453:461	arg1	zones					463:467	CS or hydroxyapatite -enriched zones	432:467	CS or hydroxyapatite -enriched zones	432:467	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	4	40	theme	polymeric	957:965	arg1	material					967:974	a polymeric material	955:974	a polymeric material with a molecular weight of 67 kDa	955:1008	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	2	41	with	layers	325:330	arg1	prevalence					337:346	prevalence	337:346	prevalence in organic tissue or mineralized	337:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	6	42	theme	major	1326:1330	arg1	component					1332:1340	major component	1326:1340	major component (85%)	1326:1346	Hydroxyapatite as major component (85%) was also obtained for jaws directly subjected to the thermal treatment.					
32275990	6	42	theme	major	1326:1330	arg1	%					1345:1345	85%	1343:1345	85%	1343:1345	Hydroxyapatite as major component (85%) was also obtained for jaws directly subjected to the thermal treatment.					
32275990	2	43	theme	hydroxyapatite	438:451	arg1	structure					268:276	The sandwich-type structure	250:276	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	250:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	2	43	theme	hydroxyapatite	438:451	arg1	zones					463:467	CS or hydroxyapatite -enriched zones	432:467	CS or hydroxyapatite -enriched zones	432:467	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	3	44	theme	obtaining	797:805	arg1	hydroxyapatite					782:795	hydroxyapatite obtaining	782:805	hydroxyapatite obtaining	782:805	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	4	45	theme	Exchange	920:927	arg1	Chromatography					929:942	Strong Anion Exchange Chromatography	907:942	Strong Anion Exchange Chromatography	907:942	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	0	46	from	jaw	66:68	arg1	hydroxyapatite					24:37	hydroxyapatite	24:37	hydroxyapatite	24:37	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	0	46	from	jaw	66:68	arg1	sulfate					12:18	Chondroitin sulfate	0:18	Chondroitin sulfate	0:18	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	4	47	theme	Gel	845:847	arg1	Chromatography					860:873	Gel Permeation Chromatography	845:873	Gel Permeation Chromatography	845:873	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	3	48	theme	thermal	760:766	arg1	treatment					768:776	controlled thermal treatment	749:776	controlled thermal treatment for hydroxyapatite obtaining	749:805	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	0	49	theme	glauca	53:58	arg1	jaw					66:68	Prionace glauca shark jaw	44:68	Prionace glauca shark jaw	44:68	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	4	50	theme	Anion	914:918	arg1	Chromatography					929:942	Strong Anion Exchange Chromatography	907:942	Strong Anion Exchange Chromatography	907:942	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	3	51	theme	chemical	648:655	arg1	precipitation					657:669	chemical precipitation	648:669	chemical precipitation	648:669	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	3	52	theme	biomaterials	555:566	arg1	recovery					538:545	the recovery	534:545	the recovery of both biomaterials and their purification process	534:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	4	53	theme	non-sulfated	1155:1166	arg1	units					1168:1172	non-sulfated units	1155:1172	non-sulfated units	1155:1172	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	1	54	theme	present	127:133	arg1	work					135:138	the present work	123:138	the present work	123:138	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
32275990	2	55	from	structure	268:276	arg1	section					287:293	cross section	281:293	cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized	281:379	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	4	56	theme	purified	812:819	arg1	CS					821:822	The purified CS	808:822	The purified CS	808:822	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	7	57	theme	centrifugation	1478:1491	arg1	influence					1436:1444	the influence	1432:1444	the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction	1432:1534	This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction.					
32275990	7	58	from	influence	1436:1444	arg1	composition					1500:1510	the composition	1496:1510	the composition of the mineral fraction	1496:1534	This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction.					
32275990	4	59	theme	prevalent	1015:1023	arg1	sulfation					1035:1043	prevalent 6S-GalNAc sulfation	1015:1043	prevalent 6S-GalNAc sulfation (68%)	1015:1049	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	4	59	theme	prevalent	1015:1023	arg1	%					1048:1048	68%	1046:1048	68%	1046:1048	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	5	60	theme	calcium	1238:1244	arg1	phosphate					1246:1254	biphasic 60:40 porous calcium phosphate	1216:1254	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	1	61	theme	chondroitin	202:212	arg1	sulfate					214:220	chondroitin sulfate	202:220	chondroitin sulfate	202:220	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
32275990	7	62	theme	enzymatic	1453:1461	arg1	hydrolysis					1463:1472	the enzymatic hydrolysis	1449:1472	the enzymatic hydrolysis	1449:1472	This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction.					
32275990	3	63	theme	sequential	513:522	arg1	steps					524:528	sequential steps	513:528	sequential steps for the recovery of both biomaterials and their purification process	513:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	5	64	theme	phosphate	1246:1254	arg1	whitlockite/β-TCP					1275:1291	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP	1205:1291	In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed.					
32275990	1	65	theme	sulfate	214:220	arg1	source					187:192	a source	185:192	a source of both chondroitin sulfate and bioapatite	185:235	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
32275990	4	66	theme	67 kDa	1003:1008	arg1	weight					993:998	a molecular weight	981:998	a molecular weight of 67 kDa	981:1008	The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units.					
32275990	7	67	theme	hydrolysis	1463:1472	arg1	influence					1436:1444	the influence	1432:1444	the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction	1432:1534	This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fraction.					
32275990	0	68	dep	sulfate	12:18	arg1	Physicochemical					71:85	Physicochemical	71:85	Physicochemical	71:85	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	0	68	dep	sulfate	12:18	arg1	characterization					102:117	structural characterization	91:117	structural characterization	91:117	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	2	69	theme	organic	351:357	arg1	tissue					359:364	organic tissue	351:364	organic tissue	351:364	The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones.					
32275990	0	70	theme	shark	60:64	arg1	jaw					66:68	Prionace glauca shark jaw	44:68	Prionace glauca shark jaw	44:68	Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization.					
32275990	3	71	theme	process	591:597	arg1	recovery					538:545	the recovery	534:545	the recovery of both biomaterials and their purification process	534:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	3	72	theme	optimized	492:500	arg1	result					473:478	result	473:478	result of this	473:486	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	3	72	theme	optimized	492:500	arg1	process					502:508	an optimized process	489:508	an optimized process in sequential steps for the recovery of both biomaterials and their purification process	489:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	3	73	from	process	502:508	arg1	steps					524:528	sequential steps	513:528	sequential steps for the recovery of both biomaterials and their purification process	513:597	As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining.					
32275990	1	74	theme	bioapatite	226:235	arg1	source					187:192	a source	185:192	a source of both chondroitin sulfate and bioapatite	185:235	In the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored.					
34536342	7	0	theme	histone	1322:1328	arg1	deacetylase					1330:1340	histone deacetylase	1322:1340	histone deacetylase (HDAC3) activity in colon	1322:1366	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	0	theme	histone	1322:1328	arg1	HDAC3					1343:1347	HDAC3	1343:1347	HDAC3	1343:1347	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	6	1	theme	microbiota	994:1003	arg1	composition					975:985	the composition	971:985	the composition of gut microbiota	971:1003	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	5	2	theme	high-cellulose	833:846	arg1	mice					853:856	high-cellulose diet mice	833:856	high-cellulose diet mice	833:856	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	6	3	theme	gut	990:992	arg1	microbiota					994:1003	gut microbiota	990:1003	gut microbiota	990:1003	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	7	4	theme	modulating	1205:1214	arg1	factors					1253:1259	its modulating to serotonin synthesis, neurotrophic factors	1201:1259	its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation	1201:1301	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	4	theme	modulating	1205:1214	arg1	expression					1261:1270	expression	1261:1270	expression	1261:1270	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	4	theme	modulating	1205:1214	arg1	differentiation					1287:1301	immunocyte differentiation	1276:1301	immunocyte differentiation	1276:1301	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	3	5	from	cytokines	540:548	arg1	colon					553:557	colon	553:557	colon of mice	553:565	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	2	6	theme	GI	368:369	arg1	function					371:378	GI function	368:378	GI function	368:378	However, how dose dietary fiber modulate inflammation and crosstalk influence GI function has not been explained in detail.					
34536342	1	7	theme	GI	276:277	arg1	motility					280:287	altered gastrointestinal (GI) motility	250:287	altered gastrointestinal (GI) motility	250:287	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	5	8	theme	metabolomics	768:779	arg1	analysis					781:788	metabolomics analysis	768:788	metabolomics analysis	768:788	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	7	9	theme	acetate	1183:1189	arg1	administration					1165:1178	Orally administration	1158:1178	Orally administration of acetate	1158:1189	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	10	from	activity	1350:1357	arg1	colon					1362:1366	colon	1362:1366	colon	1362:1366	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	8	11	theme	intestinal	1471:1480	arg1	homeostasis					1482:1492	intestinal homeostasis	1471:1492	intestinal homeostasis	1471:1492	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	6	12	theme	Firmicutes	1045:1054	arg1	ratio					1036:1040	the ratio	1032:1040	the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate	1032:1155	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	0	13	theme	serotonin	102:110	arg1	biosynthesis					112:123	serotonin biosynthesis	102:123	serotonin biosynthesis	102:123	High cellulose diet promotes intestinal motility through regulating intestinal immune homeostasis and serotonin biosynthesis.					
34536342	9	14	theme	dietary	1645:1651	arg1	diet					1597:1600	rich cellulose diet	1582:1600	rich cellulose diet	1582:1600	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	14	theme	dietary	1645:1651	arg1	supplement					1613:1622	acetate supplement	1605:1622	acetate supplement	1605:1622	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	14	theme	dietary	1645:1651	arg1	advice					1653:1658	dietary advice	1645:1658	dietary advice	1645:1658	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	15	theme	motility	1693:1700	arg1	insufficiency					1702:1714	clinically intestinal motility insufficiency	1671:1714	clinically intestinal motility insufficiency	1671:1714	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	5	16	theme	tryptophan	807:816	arg1	metabolites					818:828	increased tryptophan metabolites	797:828	increased tryptophan metabolites	797:828	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	4	17	theme	serotonin	630:638	arg1	synthesis					617:625	synthesis	617:625	synthesis of serotonin	617:638	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	4	17	theme	serotonin	630:638	arg1	expression					644:653	expression	644:653	expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility	644:752	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	4	18	theme	intestinal	734:743	arg1	motility					745:752	intestinal motility	734:752	intestinal motility	734:752	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	5	19	theme	serotonin	893:901	arg1	biosynthesis					903:914	serotonin biosynthesis	893:914	serotonin biosynthesis	893:914	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	3	20	theme	mice	562:565	arg1	colon					553:557	colon	553:557	colon of mice	553:565	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	9	21	theme	cellulose	1587:1595	arg1	diet					1597:1600	rich cellulose diet	1582:1600	rich cellulose diet	1582:1600	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	21	theme	cellulose	1587:1595	arg1	supplement					1613:1622	acetate supplement	1605:1622	acetate supplement	1605:1622	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	21	theme	cellulose	1587:1595	arg1	advice					1653:1658	dietary advice	1645:1658	dietary advice	1645:1658	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	3	22	theme	intestinal	447:456	arg1	motility					458:465	intestinal motility	447:465	intestinal motility	447:465	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	5	23	theme	increased	797:805	arg1	metabolites					818:828	increased tryptophan metabolites	797:828	increased tryptophan metabolites	797:828	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	7	24	theme	immunocyte	1276:1285	arg1	factors					1253:1259	its modulating to serotonin synthesis, neurotrophic factors	1201:1259	its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation	1201:1301	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	24	theme	immunocyte	1276:1285	arg1	differentiation					1287:1301	immunocyte differentiation	1276:1301	immunocyte differentiation	1276:1301	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	0	25	theme	cellulose	5:13	arg1	diet					15:18	High cellulose diet	0:18	High cellulose diet	0:18	High cellulose diet promotes intestinal motility through regulating intestinal immune homeostasis and serotonin biosynthesis.					
34536342	1	26	attach	linked	173:178	arg1	inflammatory					183:194	inflammatory	183:194	inflammatory	183:194	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	1	26	attach	linked	173:178	arg1	diseases					208:215	nervous diseases	200:215	nervous diseases	200:215	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	1	26	attach	linked	173:178	arg2	fiber					156:160	dietary fiber	148:160	dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility	148:287	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	7	27	theme	deacetylase	1330:1340	arg1	activity					1350:1357	histone deacetylase (HDAC3) activity	1322:1357	histone deacetylase (HDAC3) activity in colon	1322:1366	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	3	28	from	immunocytes	511:521	arg1	colon					553:557	colon	553:557	colon of mice	553:565	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	8	29	theme	nervous	1506:1512	arg1	system					1514:1519	enteric nervous system	1498:1519	enteric nervous system	1498:1519	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	1	30	theme	nervous	200:206	arg1	diseases					208:215	nervous diseases	200:215	nervous diseases	200:215	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	0	31	theme	High	0:3	arg1	diet					15:18	High cellulose diet	0:18	High cellulose diet	0:18	High cellulose diet promotes intestinal motility through regulating intestinal immune homeostasis and serotonin biosynthesis.					
34536342	4	32	theme	high-cellulose	587:600	arg1	diet					602:605	high-cellulose diet	587:605	high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility	587:752	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	8	33	theme	enteric	1498:1504	arg1	system					1514:1519	enteric nervous system	1498:1519	enteric nervous system	1498:1519	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	7	34	dep	factors	1253:1259	arg1	factors					1253:1259	its modulating to serotonin synthesis, neurotrophic factors	1201:1259	its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation	1201:1301	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	34	dep	factors	1253:1259	arg1	expression					1261:1270	expression	1261:1270	expression	1261:1270	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	7	34	dep	factors	1253:1259	arg1	differentiation					1287:1301	immunocyte differentiation	1276:1301	immunocyte differentiation	1276:1301	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	8	35	theme	high-cellulose	1404:1417	arg1	diet					1419:1422	high-cellulose diet	1404:1422	high-cellulose diet	1404:1422	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	2	36	theme	dietary	308:314	arg1	fiber					316:320	dose dietary fiber	303:320	dose dietary fiber	303:320	However, how dose dietary fiber modulate inflammation and crosstalk influence GI function has not been explained in detail.					
34536342	9	37	theme	acetate	1605:1611	arg1	diet					1597:1600	rich cellulose diet	1582:1600	rich cellulose diet	1582:1600	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	37	theme	acetate	1605:1611	arg1	supplement					1613:1622	acetate supplement	1605:1622	acetate supplement	1605:1622	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	37	theme	acetate	1605:1611	arg1	advice					1653:1658	dietary advice	1645:1658	dietary advice	1645:1658	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	7	38	dep	modulating	1205:1214	arg1	neurotrophic					1240:1251	neurotrophic	1240:1251	neurotrophic	1240:1251	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	6	39	theme	high-cellulose	943:956	arg1	diet					958:961	high-cellulose diet	943:961	high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate	943:1155	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	2	40	theme	dose	303:306	arg1	fiber					316:320	dose dietary fiber	303:320	dose dietary fiber	303:320	However, how dose dietary fiber modulate inflammation and crosstalk influence GI function has not been explained in detail.					
34536342	3	41	theme	upregulated	483:493	arg1	immunocytes					511:521	upregulated proinflammatory immunocytes	483:521	upregulated proinflammatory immunocytes	483:521	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	5	42	theme	diet	848:851	arg1	mice					853:856	high-cellulose diet mice	833:856	high-cellulose diet mice	833:856	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	8	43	theme	acetate	1535:1541	arg1	production					1543:1552	acetate production	1535:1552	acetate production	1535:1552	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	6	44	theme	acids	1123:1127	arg1	concentration					1088:1100	concentration	1088:1100	concentration of short-chain fatty acids (SCFAs), especially acetate	1088:1155	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	6	44	theme	acids	1123:1127	arg1	Bacteroidetes					1059:1071	Bacteroidetes	1059:1071	Bacteroidetes	1059:1071	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	0	45	theme	intestinal	29:38	arg1	motility					40:47	intestinal motility	29:47	intestinal motility	29:47	High cellulose diet promotes intestinal motility through regulating intestinal immune homeostasis and serotonin biosynthesis.					
34536342	3	46	theme	fiber-free	423:432	arg1	diet					434:437	fiber-free diet	423:437	fiber-free diet	423:437	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	4	47	theme	factors	671:677	arg1	synthesis					617:625	synthesis	617:625	synthesis of serotonin	617:638	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	4	47	theme	factors	671:677	arg1	expression					644:653	expression	644:653	expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility	644:752	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	6	48	theme	fatty	1117:1121	arg1	SCFAs					1130:1134	SCFAs	1130:1134	SCFAs	1130:1134	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	6	48	theme	fatty	1117:1121	arg1	acids					1123:1127	short-chain fatty acids	1105:1127	short-chain fatty acids (SCFAs)	1105:1135	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	6	48	theme	fatty	1117:1121	arg1	acetate					1149:1155	acetate	1149:1155	acetate	1149:1155	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	6	49	theme	Further	917:923	arg1	analysis					925:932	Further analysis	917:932	Further analysis	917:932	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	7	50	theme	serotonin	1219:1227	arg1	synthesis					1229:1237	serotonin synthesis	1219:1237	serotonin synthesis	1219:1237	Orally administration of acetate confirmed its modulating to serotonin synthesis, neurotrophic factors expression and immunocyte differentiation through regulating histone deacetylase (HDAC3) activity in colon.					
34536342	9	51	theme	intestinal	1682:1691	arg1	insufficiency					1702:1714	clinically intestinal motility insufficiency	1671:1714	clinically intestinal motility insufficiency	1671:1714	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	3	52	theme	inflammatory	527:538	arg1	cytokines					540:548	inflammatory cytokines	527:548	inflammatory cytokines	527:548	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	2	53	dep	modulate	322:329	arg1	influence					358:366	influence	358:366	modulate inflammation and crosstalk influence GI function	322:378	However, how dose dietary fiber modulate inflammation and crosstalk influence GI function has not been explained in detail.					
34536342	6	54	theme	short-chain	1105:1115	arg1	SCFAs					1130:1134	SCFAs	1130:1134	SCFAs	1130:1134	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	6	54	theme	short-chain	1105:1115	arg1	acids					1123:1127	short-chain fatty acids	1105:1127	short-chain fatty acids (SCFAs)	1105:1135	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	6	54	theme	short-chain	1105:1115	arg1	acetate					1149:1155	acetate	1149:1155	acetate	1149:1155	Further analysis revealed high-cellulose diet changed the composition of gut microbiota, in particular by altering the ratio of Firmicutes to Bacteroidetes, consequently, concentration of short-chain fatty acids (SCFAs), especially acetate.					
34536342	8	55	theme	HDAC3	1558:1562	arg1	inhibition					1564:1573	HDAC3 inhibition	1558:1573	HDAC3 inhibition	1558:1573	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	1	56	theme	dietary	148:154	arg1	fiber					156:160	dietary fiber	148:160	dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility	148:287	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	3	57	theme	proinflammatory	495:509	arg1	immunocytes					511:521	upregulated proinflammatory immunocytes	483:521	upregulated proinflammatory immunocytes	483:521	We found fiber-free diet reduced intestinal motility, accompanied by upregulated proinflammatory immunocytes and inflammatory cytokines in colon of mice.					
34536342	0	58	theme	immune	79:84	arg1	homeostasis					86:96	intestinal immune homeostasis	68:96	intestinal immune homeostasis	68:96	High cellulose diet promotes intestinal motility through regulating intestinal immune homeostasis and serotonin biosynthesis.					
34536342	5	59	from	metabolites	818:828	arg1	mice					853:856	high-cellulose diet mice	833:856	high-cellulose diet mice	833:856	In addition, metabolomics analysis showed increased tryptophan metabolites in high-cellulose diet mice, which happened to be required for serotonin biosynthesis.					
34536342	8	60	theme	intestinal	1432:1441	arg1	motility					1443:1450	intestinal motility	1432:1450	intestinal motility	1432:1450	Together, our results demonstrated high-cellulose diet promote intestinal motility through regulating intestinal homeostasis and enteric nervous system by increasing acetate production and HDAC3 inhibition.					
34536342	9	61	theme	rich	1582:1585	arg1	diet					1597:1600	rich cellulose diet	1582:1600	rich cellulose diet	1582:1600	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	61	theme	rich	1582:1585	arg1	supplement					1613:1622	acetate supplement	1605:1622	acetate supplement	1605:1622	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	9	61	theme	rich	1582:1585	arg1	advice					1653:1658	dietary advice	1645:1658	dietary advice	1645:1658	Thus, rich cellulose diet or acetate supplement can be considered as dietary advice to improve clinically intestinal motility insufficiency.					
34536342	1	62	theme	altered	250:256	arg1	motility					280:287	altered gastrointestinal (GI) motility	250:287	altered gastrointestinal (GI) motility	250:287	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
34536342	0	63	theme	intestinal	68:77	arg1	homeostasis					86:96	intestinal immune homeostasis	68:96	intestinal immune homeostasis	68:96	High cellulose diet promotes intestinal motility through regulating intestinal immune homeostasis and serotonin biosynthesis.					
34536342	4	64	theme	neurotrophic	658:669	arg1	factors					671:677	neurotrophic factors	658:677	neurotrophic factors	658:677	We also discovered high-cellulose diet increased synthesis of serotonin and expression of neurotrophic factors, both of that have been reported involved in promoting intestinal motility.					
34536342	1	65	theme	gastrointestinal	258:273	arg1	motility					280:287	altered gastrointestinal (GI) motility	250:287	altered gastrointestinal (GI) motility	250:287	It is widely accepted dietary fiber intimately linked to inflammatory and nervous diseases, which often been described with altered gastrointestinal (GI) motility.					
33103953	2	0	theme	%	435:435	arg1	solubilization					413:426	the highest hemicellulose solubilization	387:426	the highest hemicellulose solubilization of 56.0%	387:435	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	2	0	theme	%	435:435	arg1	AP					442:443	AP	442:443	AP of the highest lignin removal of 49.6%	442:482	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	3	1	theme	methods	653:659	arg1	sequence					624:631	the sequence	620:631	the sequence of the pretreatment methods	620:659	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	4	2	theme	%	961:961	arg1	recovery					945:952	the highest cellulose recovery	923:952	the highest cellulose recovery of 94.0%	923:961	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	3	3	theme	biomass	759:765	arg1	characteristics					704:718	substrate characteristics	694:718	substrate characteristics	694:718	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	3	3	theme	biomass	759:765	arg1	efficiency					745:754	enzymatic hydrolysis efficiency	724:754	enzymatic hydrolysis efficiency of biomass	724:765	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	8	4	theme	structure	1409:1417	arg1	alteration					1385:1394	alteration	1385:1394	alteration of cellulose structure	1385:1417	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	8	4	theme	structure	1409:1417	arg1	relocation					1360:1369	partial relocation	1352:1369	partial relocation of lignin	1352:1379	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	8	5	theme	cellulose	1399:1407	arg1	structure					1409:1417	cellulose structure	1399:1417	cellulose structure	1399:1417	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	8	6	theme	lignocellulosic	1483:1497	arg1	biomass					1499:1505	the lignocellulosic biomass	1479:1505	the lignocellulosic biomass	1479:1505	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	8	7	theme	barrier	1292:1298	arg1	removal					1268:1274	complete removal	1259:1274	complete removal of the physical barrier of lignin and hemicellulose	1259:1326	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	1	8	theme	alkaline	230:237	arg1	AP					240:241	alkaline (AP)	230:242	alkaline (AP)	230:242	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	0	9	theme	shells	103:108	arg1	hydrolysis					80:89	enzymatic hydrolysis	70:89	enzymatic hydrolysis	70:89	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	0	9	theme	shells	103:108	arg1	composition					54:64	composition	54:64	composition	54:64	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	0	10	from	Effect	0:5	arg1	hydrolysis					80:89	enzymatic hydrolysis	70:89	enzymatic hydrolysis	70:89	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	0	10	from	Effect	0:5	arg1	composition					54:64	composition	54:64	composition	54:64	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	4	11	theme	60.7	813:816	arg1	%					817:817	%	817:817	%	817:817	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	8	12	theme	complete	1259:1266	arg1	removal					1268:1274	complete removal	1259:1274	complete removal of the physical barrier of lignin and hemicellulose	1259:1326	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	6	13	theme	maximum	1051:1057	arg1	recovery					1067:1074	The maximum glucose recovery	1047:1074	The maximum glucose recovery of 54.9%	1047:1083	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	0	14	theme	hazelnut	94:101	arg1	shells					103:108	hazelnut shells	94:108	hazelnut shells	94:108	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	5	15	theme	hazelnut	1008:1015	arg1	shells					1017:1022	pretreated hazelnut shells	997:1022	pretreated hazelnut shells	997:1022	Structural properties of raw and pretreated hazelnut shells were observed by FTIR.					
33103953	3	16	theme	pretreatment	640:651	arg1	methods					653:659	the pretreatment methods	636:659	the pretreatment methods	636:659	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	2	17	theme	%	368:368	arg1	recovery					352:359	the highest cellulose recovery	330:359	the highest cellulose recovery of 98.1%	330:368	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	6	18	theme	%	1083:1083	arg1	recovery					1067:1074	The maximum glucose recovery	1047:1074	The maximum glucose recovery of 54.9%	1047:1083	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	1	19	contain	containing	142:151	arg1	shells					120:125	Hazelnut shells	111:125	Hazelnut shells	111:125	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	19	contain	containing	142:151	arg2	biomass					153:159	biomass	153:159	biomass	153:159	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	19	contain	containing	142:151	arg1	lignin					135:140	a high lignin	128:140	a high lignin containing biomass	128:159	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	2	20	theme	cellulose	342:350	arg1	recovery					352:359	the highest cellulose recovery	330:359	the highest cellulose recovery of 98.1%	330:368	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	4	21	theme	delignification	780:794	arg1	terms					771:775	terms	771:775	terms of delignification	771:794	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	4	22	theme	%	897:897	arg1	removal					882:888	the highest hemicellulose removal	856:888	the highest hemicellulose removal of 93.8% and DAP-LHW	856:909	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	3	23	contain	had	661:663	arg2	impact					679:684	a significant impact	665:684	a significant impact	665:684	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	3	23	contain	had	661:663	arg1	sequence					624:631	the sequence	620:631	the sequence of the pretreatment methods	620:659	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	5	24	theme	shells	1017:1022	arg1	properties					975:984	Structural properties	964:984	Structural properties of raw and pretreated hazelnut shells	964:1022	Structural properties of raw and pretreated hazelnut shells were observed by FTIR.					
33103953	2	25	theme	highest	334:340	arg1	recovery					352:359	the highest cellulose recovery	330:359	the highest cellulose recovery of 98.1%	330:368	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	4	26	theme	cellulose	935:943	arg1	recovery					945:952	the highest cellulose recovery	923:952	the highest cellulose recovery of 94.0%	923:961	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	6	27	located	observed	1089:1096	arg1	samples					1120:1126	DAP-LHW pretreated samples	1101:1126	DAP-LHW pretreated samples	1101:1126	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	6	27	located	observed	1089:1096	arg2	recovery					1067:1074	The maximum glucose recovery	1047:1074	The maximum glucose recovery of 54.9%	1047:1083	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	3	28	theme	two-step	495:502	arg1	pretreatment					504:515	two-step pretreatment	495:515	two-step pretreatment on hazelnut shells	495:534	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	3	29	from	pretreatment	504:515	arg1	shells					529:534	hazelnut shells	520:534	hazelnut shells	520:534	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	8	30	theme	partial	1352:1358	arg1	relocation					1360:1369	partial relocation	1352:1369	partial relocation of lignin	1352:1379	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	0	31	theme	pretreatment	21:32	arg1	combinations					34:45	sequential pretreatment combinations	10:45	sequential pretreatment combinations	10:45	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	3	32	theme	significant	667:677	arg1	impact					679:684	a significant impact	665:684	a significant impact	665:684	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	8	33	theme	hemicellulose	1314:1326	arg1	barrier					1292:1298	the physical barrier	1279:1298	the physical barrier of lignin and hemicellulose	1279:1326	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	6	34	theme	glucose	1059:1065	arg1	recovery					1067:1074	The maximum glucose recovery	1047:1074	The maximum glucose recovery of 54.9%	1047:1083	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	3	35	theme	hazelnut	520:527	arg1	shells					529:534	hazelnut shells	520:534	hazelnut shells	520:534	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	7	36	theme	10.2 g	1215:1220	arg1	glucose					1222:1228	10.2 g glucose	1215:1228	10.2 g glucose	1215:1228	For this pretreatment combination, almost 1.8 MJ total energy was required to recover 10.2 g glucose.					
33103953	0	37	theme	sequential	10:19	arg1	combinations					34:45	sequential pretreatment combinations	10:45	sequential pretreatment combinations	10:45	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	1	38	theme	dilute	248:253	arg1	pretreatments					260:272	dilute acid pretreatments	248:272	dilute acid pretreatments	248:272	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	4	39	dep	showed	849:854	arg1	resulted					911:918	resulted	911:918	showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%	849:961	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	3	40	theme	enhanced	565:572	arg1	action					574:579	an enhanced action	562:579	an enhanced action of the second pretreatment	562:606	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	1	41	theme	Hazelnut	111:118	arg1	shells					120:125	Hazelnut shells	111:125	Hazelnut shells	111:125	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	41	theme	Hazelnut	111:118	arg1	lignin					135:140	a high lignin	128:140	a high lignin containing biomass	128:159	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	8	42	theme	physical	1283:1290	arg1	barrier					1292:1298	the physical barrier	1279:1298	the physical barrier of lignin and hemicellulose	1279:1326	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	1	43	theme	sequential	195:204	arg1	LHW					224:226	LHW	224:226	LHW	224:226	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	43	theme	sequential	195:204	arg1	water					217:221	sequential liquid hot water	195:221	sequential liquid hot water (LHW)	195:227	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	43	theme	sequential	195:204	arg1	DAP					275:277	DAP	275:277	DAP	275:277	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	44	theme	acid	255:258	arg1	pretreatments					260:272	dilute acid pretreatments	248:272	dilute acid pretreatments	248:272	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	2	45	theme	removal	467:473	arg1	solubilization					413:426	the highest hemicellulose solubilization	387:426	the highest hemicellulose solubilization of 56.0%	387:435	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	2	45	theme	removal	467:473	arg1	AP					442:443	AP	442:443	AP of the highest lignin removal of 49.6%	442:482	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	5	46	theme	raw	989:991	arg1	properties					975:984	Structural properties	964:984	Structural properties of raw and pretreated hazelnut shells	964:1022	Structural properties of raw and pretreated hazelnut shells were observed by FTIR.					
33103953	0	47	dep	composition	54:64	arg1	the					50:52	the	50:52	the	50:52	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	1	48	theme	liquid	206:211	arg1	LHW					224:226	LHW	224:226	LHW	224:226	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	48	theme	liquid	206:211	arg1	water					217:221	sequential liquid hot water	195:221	sequential liquid hot water (LHW)	195:227	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	48	theme	liquid	206:211	arg1	DAP					275:277	DAP	275:277	DAP	275:277	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	0	49	theme	combinations	34:45	arg1	Effect					0:5	Effect	0:5	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.	0:109	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	2	50	theme	lignin	460:465	arg1	removal					467:473	the highest lignin removal	448:473	the highest lignin removal of 49.6%	448:482	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	8	51	theme	lignin	1374:1379	arg1	alteration					1385:1394	alteration	1385:1394	alteration of cellulose structure	1385:1417	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	8	51	theme	lignin	1374:1379	arg1	relocation					1360:1369	partial relocation	1352:1369	partial relocation of lignin	1352:1379	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	8	52	theme	lignin	1303:1308	arg1	barrier					1292:1298	the physical barrier	1279:1298	the physical barrier of lignin and hemicellulose	1279:1326	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	1	53	theme	hot	213:215	arg1	LHW					224:226	LHW	224:226	LHW	224:226	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	53	theme	hot	213:215	arg1	water					217:221	sequential liquid hot water	195:221	sequential liquid hot water (LHW)	195:227	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	53	theme	hot	213:215	arg1	DAP					275:277	DAP	275:277	DAP	275:277	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	2	54	theme	hemicellulose	399:411	arg1	solubilization					413:426	the highest hemicellulose solubilization	387:426	the highest hemicellulose solubilization of 56.0%	387:435	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	2	55	theme	highest	452:458	arg1	removal					467:473	the highest lignin removal	448:473	the highest lignin removal of 49.6%	448:482	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	4	56	theme	lignin	819:824	arg1	removal					826:832	60.7% lignin removal	813:832	60.7% lignin removal	813:832	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	5	57	theme	pretreated	997:1006	arg1	shells					1017:1022	pretreated hazelnut shells	997:1022	pretreated hazelnut shells	997:1022	Structural properties of raw and pretreated hazelnut shells were observed by FTIR.					
33103953	4	58	theme	highest	860:866	arg1	removal					882:888	the highest hemicellulose removal	856:888	the highest hemicellulose removal of 93.8% and DAP-LHW	856:909	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	2	59	theme	single	291:296	arg1	pretreatments					298:310	the single pretreatments	287:310	the single pretreatments	287:310	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	2	60	theme	highest	391:397	arg1	solubilization					413:426	the highest hemicellulose solubilization	387:426	the highest hemicellulose solubilization of 56.0%	387:435	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	4	61	theme	%	817:817	arg1	removal					826:832	60.7% lignin removal	813:832	60.7% lignin removal	813:832	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	3	62	theme	second	588:593	arg1	pretreatment					595:606	the second pretreatment	584:606	the second pretreatment	584:606	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	4	63	theme	hemicellulose	868:880	arg1	removal					882:888	the highest hemicellulose removal	856:888	the highest hemicellulose removal of 93.8% and DAP-LHW	856:909	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	3	64	theme	pretreatment	595:606	arg1	action					574:579	an enhanced action	562:579	an enhanced action of the second pretreatment	562:606	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	8	65	from	biomass	1499:1505	arg1	recovery					1465:1472	the sugar recovery	1455:1472	the sugar recovery from the lignocellulosic biomass	1455:1505	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	7	66	theme	total	1178:1182	arg1	energy					1184:1189	almost 1.8 MJ total energy	1164:1189	almost 1.8 MJ total energy	1164:1189	For this pretreatment combination, almost 1.8 MJ total energy was required to recover 10.2 g glucose.					
33103953	3	67	theme	enzymatic	724:732	arg1	efficiency					745:754	enzymatic hydrolysis efficiency	724:754	enzymatic hydrolysis efficiency of biomass	724:765	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	2	68	theme	%	482:482	arg1	removal					467:473	the highest lignin removal	448:473	the highest lignin removal of 49.6%	448:482	Among the single pretreatments, LHW demonstrated the highest cellulose recovery of 98.1%, DAP resulted in the highest hemicellulose solubilization of 56.0%, and AP of the highest lignin removal of 49.6%.					
33103953	5	69	theme	Structural	964:973	arg1	properties					975:984	Structural properties	964:984	Structural properties of raw and pretreated hazelnut shells	964:1022	Structural properties of raw and pretreated hazelnut shells were observed by FTIR.					
33103953	6	70	theme	pretreated	1109:1118	arg1	samples					1120:1126	DAP-LHW pretreated samples	1101:1126	DAP-LHW pretreated samples	1101:1126	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	1	71	theme	high	130:133	arg1	shells					120:125	Hazelnut shells	111:125	Hazelnut shells	111:125	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	1	71	theme	high	130:133	arg1	lignin					135:140	a high lignin	128:140	a high lignin containing biomass	128:159	Hazelnut shells, a high lignin containing biomass, were subjected to individual and sequential liquid hot water (LHW), alkaline (AP) and dilute acid pretreatments (DAP).					
33103953	3	72	theme	hydrolysis	734:743	arg1	efficiency					745:754	enzymatic hydrolysis efficiency	724:754	enzymatic hydrolysis efficiency of biomass	724:765	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
33103953	0	73	theme	enzymatic	70:78	arg1	hydrolysis					80:89	enzymatic hydrolysis	70:89	enzymatic hydrolysis	70:89	Effect of sequential pretreatment combinations on the composition and enzymatic hydrolysis of hazelnut shells.					
33103953	6	74	theme	DAP-LHW	1101:1107	arg1	samples					1120:1126	DAP-LHW pretreated samples	1101:1126	DAP-LHW pretreated samples	1101:1126	The maximum glucose recovery of 54.9% was observed in DAP-LHW pretreated samples.					
33103953	8	75	theme	sugar	1459:1463	arg1	recovery					1465:1472	the sugar recovery	1455:1472	the sugar recovery from the lignocellulosic biomass	1455:1505	The findings indicated that complete removal of the physical barrier of lignin and hemicellulose might not be essential; partial relocation of lignin and alteration of cellulose structure may also be efficient in increasing the sugar recovery from the lignocellulosic biomass.					
33103953	7	76	theme	pretreatment	1138:1149	arg1	combination					1151:1161	this pretreatment combination	1133:1161	this pretreatment combination	1133:1161	For this pretreatment combination, almost 1.8 MJ total energy was required to recover 10.2 g glucose.					
33103953	4	77	theme	highest	927:933	arg1	recovery					945:952	the highest cellulose recovery	923:952	the highest cellulose recovery of 94.0%	923:961	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	4	78	theme	DAP-LHW	903:909	arg1	removal					882:888	the highest hemicellulose removal	856:888	the highest hemicellulose removal of 93.8% and DAP-LHW	856:909	In terms of delignification, AP-LHW achieved 60.7% lignin removal, while LHW-DAP showed the highest hemicellulose removal of 93.8% and DAP-LHW resulted in the highest cellulose recovery of 94.0%.					
33103953	3	79	theme	substrate	694:702	arg1	characteristics					704:718	substrate characteristics	694:718	substrate characteristics	694:718	Employing two-step pretreatment on hazelnut shells, in general, demonstrated an enhanced action of the second pretreatment; therefore, the sequence of the pretreatment methods had a significant impact on both substrate characteristics and enzymatic hydrolysis efficiency of biomass.					
34597693	6	0	theme	droplet	904:910	arg1	diameter					912:919	the mean droplet diameter	895:919	the mean droplet diameter of the emulsions	895:936	The results showed that no significant change in the mean droplet diameter of the emulsions was observed after storage for a month.					
34597693	5	1	theme	system	823:828	arg1	activity					797:804	the antioxidant activity	781:804	the antioxidant activity of the emulsions system	781:828	Thereafter, vit-D3 was encapsulated, and the antioxidant activity of the emulsions system were evaluated.					
34597693	8	2	theme	emulsion	1293:1300	arg1	destabilization					1270:1284	complete destabilization	1261:1284	complete destabilization of the emulsion	1261:1300	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	0	3	theme	Pickering	88:96	arg1	emulsions					98:106	Pickering emulsions	88:106	Pickering emulsions	88:106	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	6	4	theme	mean	899:902	arg1	diameter					912:919	the mean droplet diameter	895:919	the mean droplet diameter of the emulsions	895:936	The results showed that no significant change in the mean droplet diameter of the emulsions was observed after storage for a month.					
34597693	7	5	theme	50	1113:1114	arg1	%					1115:1115	%	1115:1115	%	1115:1115	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	3	6	from	temperature	550:560	arg1	preparation					498:508	preparation	498:508	preparation	498:508	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	1	7	theme	bioactive	276:284	arg1	compounds					286:294	bioactive compounds	276:294	bioactive compounds with antioxidant potentials	276:322	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	0	8	theme	antioxidant	113:123	arg1	activities					125:134	antioxidant activities	113:134	antioxidant activities of the encapsulated vit-D3	113:161	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	10	9	theme	significant	1390:1400	arg1	difference					1402:1411	No significant difference	1387:1411	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions	1387:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	10	10	theme	irrespective	1550:1561	arg1	capability					1518:1527	their capability	1512:1527	their capability	1512:1527	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	10	10	theme	irrespective	1550:1561	arg1	vehicles					1541:1548	delivery vehicles	1532:1548	delivery vehicles irrespective of their composition	1532:1582	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	1	11	theme	compounds	286:294	arg1	range					267:271	a wide range	260:271	a wide range of bioactive compounds with antioxidant potentials	260:322	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	7	12	theme	1:2	1102:1104	arg1	ratio					1093:1097	ZN/CS ratio	1087:1097	ZN/CS ratio of 1:2	1087:1104	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	8	13	theme	GA	1163:1164	arg1	addition					1166:1173	GA addition	1163:1173	GA addition	1163:1173	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	7	14	theme	ZN/CS	1087:1091	arg1	ratio					1093:1097	ZN/CS ratio	1087:1097	ZN/CS ratio of 1:2	1087:1104	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	2	15	dep	/chitosan	380:388	arg1	CS					391:392	CS	391:392	CS	391:392	In this work, we formulated and characterized zein (ZN)/chitosan (CS) stabilized Pickering emulsion.					
34597693	2	16	theme	stabilized	395:404	arg1	emulsion					416:423	zein (ZN)/chitosan (CS) stabilized Pickering emulsion	371:423	zein (ZN)/chitosan (CS) stabilized Pickering emulsion	371:423	In this work, we formulated and characterized zein (ZN)/chitosan (CS) stabilized Pickering emulsion.					
34597693	6	17	from	change	885:890	arg1	diameter					912:919	the mean droplet diameter	895:919	the mean droplet diameter of the emulsions	895:936	The results showed that no significant change in the mean droplet diameter of the emulsions was observed after storage for a month.					
34597693	6	18	theme	significant	873:883	arg1	change					885:890	no significant change	870:890	no significant change in the mean droplet diameter of the emulsions	870:936	The results showed that no significant change in the mean droplet diameter of the emulsions was observed after storage for a month.					
34597693	10	19	theme	radical	1425:1431	arg1	activity					1444:1451	the DPPH radical scavenging activity	1416:1451	the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions	1416:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	3	20	theme	room	545:548	arg1	temperature					550:560	room temperature	545:560	room temperature	545:560	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	10	21	theme	delivery	1532:1539	arg1	capability					1518:1527	their capability	1512:1527	their capability	1512:1527	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	10	21	theme	delivery	1532:1539	arg1	vehicles					1541:1548	delivery vehicles	1532:1548	delivery vehicles irrespective of their composition	1532:1582	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	8	22	from	increase	1192:1199	arg1	size					1216:1219	the droplet size	1204:1219	the droplet size up to some level	1204:1236	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	10	23	theme	DPPH	1420:1423	arg1	activity					1444:1451	the DPPH radical scavenging activity	1416:1451	the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions	1416:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	3	24	theme	prepared	430:437	arg1	emulsions					439:447	The prepared emulsions	426:447	The prepared emulsions	426:447	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	10	25	theme	composition	1572:1582	arg1	irrespective					1550:1561	irrespective	1550:1561	irrespective	1550:1561	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	1	26	theme	antioxidant	301:311	arg1	potentials					313:322	antioxidant potentials	301:322	antioxidant potentials	301:322	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	10	27	theme	scavenging	1433:1442	arg1	activity					1444:1451	the DPPH radical scavenging activity	1416:1451	the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions	1416:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	1	28	theme	promising	206:214	arg1	candidates					216:225	promising candidates	206:225	promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials	206:322	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	7	29	theme	significant	1140:1150	arg1	stability					1152:1160	significant stability	1140:1160	significant stability	1140:1160	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	0	30	theme	encapsulated	143:154	arg1	vit-D3					156:161	the encapsulated vit-D3	139:161	the encapsulated vit-D3	139:161	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	8	31	theme	gradual	1184:1190	arg1	increase					1192:1199	a gradual increase	1182:1199	a gradual increase in the droplet size up to some level	1182:1236	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	3	32	theme	droplet	479:485	arg1	size					487:490	droplet size	479:490	droplet size	479:490	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	5	33	theme	emulsions	813:821	arg1	system					823:828	the emulsions system	809:828	the emulsions system	809:828	Thereafter, vit-D3 was encapsulated, and the antioxidant activity of the emulsions system were evaluated.					
34597693	1	34	theme	Pickering	164:172	arg1	emulsions					174:182	Pickering emulsions	164:182	Pickering emulsions	164:182	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	10	35	from	difference	1402:1411	arg1	activity					1444:1451	the DPPH radical scavenging activity	1416:1451	the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions	1416:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	3	36	theme	prebiotic	595:603	arg1	GA					617:618	GA	617:618	GA	617:618	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	3	36	theme	prebiotic	595:603	arg1	Arabic					609:614	prebiotic gum Arabic	595:614	prebiotic gum Arabic (GA)	595:619	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	7	37	theme	%	1115:1115	arg1	contents					1121:1128	50% oil contents	1113:1128	50% oil contents exhibited significant stability	1113:1160	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	0	38	theme	rheological	27:37	arg1	properties					39:48	rheological properties	27:48	rheological properties	27:48	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	0	39	theme	vit-D3	156:161	arg1	activities					125:134	antioxidant activities	113:134	antioxidant activities of the encapsulated vit-D3	113:161	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	3	40	theme	gum	605:607	arg1	GA					617:618	GA	617:618	GA	617:618	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	3	40	theme	gum	605:607	arg1	Arabic					609:614	prebiotic gum Arabic	595:614	prebiotic gum Arabic (GA)	595:619	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	1	41	with	compounds	286:294	arg1	potentials					313:322	antioxidant potentials	301:322	antioxidant potentials	301:322	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	2	42	theme	Pickering	406:414	arg1	emulsion					416:423	zein (ZN)/chitosan (CS) stabilized Pickering emulsion	371:423	zein (ZN)/chitosan (CS) stabilized Pickering emulsion	371:423	In this work, we formulated and characterized zein (ZN)/chitosan (CS) stabilized Pickering emulsion.					
34597693	0	43	theme	zein/chitosan	53:65	arg1	particles					67:75	zein/chitosan particles	53:75	zein/chitosan particles	53:75	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	6	44	theme	emulsions	928:936	arg1	diameter					912:919	the mean droplet diameter	895:919	the mean droplet diameter of the emulsions	895:936	The results showed that no significant change in the mean droplet diameter of the emulsions was observed after storage for a month.					
34597693	7	45	theme	rheological	1020:1030	arg1	measurements					1032:1043	their rheological measurements	1014:1043	their rheological measurements	1014:1043	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	7	45	theme	rheological	1020:1030	arg1	emulsions					1063:1071	the emulsions	1059:1071	the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability	1059:1160	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	10	46	theme	emulsions	1480:1488	arg1	activity					1444:1451	the DPPH radical scavenging activity	1416:1451	the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions	1416:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	5	47	theme	antioxidant	785:795	arg1	activity					797:804	the antioxidant activity	781:804	the antioxidant activity of the emulsions system	781:828	Thereafter, vit-D3 was encapsulated, and the antioxidant activity of the emulsions system were evaluated.					
34597693	10	48	theme	vit-D3	1460:1465	arg1	emulsions					1480:1488	the vit-D3 encapsulated emulsions	1456:1488	the vit-D3 encapsulated emulsions	1456:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	8	49	dep	caused	1175:1180	arg1	led					1254:1256	led	1254:1256	led to complete destabilization of the emulsion	1254:1300	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	7	50	theme	oil	1117:1119	arg1	contents					1121:1128	50% oil contents	1113:1128	50% oil contents exhibited significant stability	1113:1160	This claim was further confirmed by their rheological measurements particularly, the emulsions prepared with ZN/CS ratio of 1:2 having 50% oil contents exhibited significant stability.					
34597693	4	51	theme	emulsions	715:723	arg1	behavior					683:690	behavior	683:690	behavior	683:690	Rheological measurements were further carried out to see the behavior and stability of these emulsions after storage.					
34597693	4	51	theme	emulsions	715:723	arg1	stability					696:704	stability	696:704	stability	696:704	Rheological measurements were further carried out to see the behavior and stability of these emulsions after storage.					
34597693	10	52	theme	encapsulated	1467:1478	arg1	emulsions					1480:1488	the vit-D3 encapsulated emulsions	1456:1488	the vit-D3 encapsulated emulsions	1456:1488	No significant difference in the DPPH radical scavenging activity of the vit-D3 encapsulated emulsions was observed, showing their capability as delivery vehicles irrespective of their composition.					
34597693	8	53	theme	droplet	1208:1214	arg1	size					1216:1219	the droplet size	1204:1219	the droplet size up to some level	1204:1236	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	8	54	theme	complete	1261:1268	arg1	destabilization					1270:1284	complete destabilization	1261:1284	complete destabilization of the emulsion	1261:1300	GA addition caused a gradual increase in the droplet size up to some level, after which it led to complete destabilization of the emulsion.					
34597693	3	55	theme	Arabic	609:614	arg1	addition					583:590	the addition	579:590	the addition of prebiotic gum Arabic (GA)	579:619	The prepared emulsions were firstly characterized by droplet size after preparation and after storage for one month at room temperature as well as after the addition of prebiotic gum Arabic (GA).					
34597693	4	56	theme	Rheological	622:632	arg1	measurements					634:645	Rheological measurements	622:645	Rheological measurements	622:645	Rheological measurements were further carried out to see the behavior and stability of these emulsions after storage.					
34597693	2	57	theme	/chitosan	380:388	arg1	emulsion					416:423	zein (ZN)/chitosan (CS) stabilized Pickering emulsion	371:423	zein (ZN)/chitosan (CS) stabilized Pickering emulsion	371:423	In this work, we formulated and characterized zein (ZN)/chitosan (CS) stabilized Pickering emulsion.					
34597693	1	58	theme	wide	262:265	arg1	range					267:271	a wide range	260:271	a wide range of bioactive compounds with antioxidant potentials	260:322	Pickering emulsions have been known to be promising candidates for encapsulating and delivering a wide range of bioactive compounds with antioxidant potentials.					
34597693	0	59	theme	particles	67:75	arg1	stability					13:21	stability	13:21	stability	13:21	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	0	59	theme	particles	67:75	arg1	properties					39:48	rheological properties	27:48	rheological properties	27:48	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
34597693	0	59	theme	particles	67:75	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3.					
32408255	0	0	theme	lamina	97:102	arg1	splendens					104:112	lamina splendens	97:112	lamina splendens	97:112	A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens.					
32408255	7	1	theme	friction	1420:1427	arg1	coefficient					1405:1415	the coefficient	1401:1415	the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage)	1401:1504	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	1	2	attach	present	184:190	arg1	interfaces					212:221	all articulating interfaces	195:221	all articulating interfaces	195:221	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	2	attach	present	184:190	arg2	films					135:139	Adsorbed lubricious films	115:139	Adsorbed lubricious films composed of biomacromolecules	115:169	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	2	attach	present	184:190	arg1	body					236:239	the human body	226:239	the human body where they provide ultralow friction and maintain normal physiological function	226:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	6	3	theme	PRG4	1159:1162	arg1	recruitment					1165:1175	glycoprotein (PRG4) recruitment	1145:1175	glycoprotein (PRG4) recruitment by Chi-C	1145:1184	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	9	4	theme	metabolic	1716:1724	arg1	activity					1726:1733	the metabolic activity	1712:1733	the metabolic activity of human chondrocytes	1712:1755	Chi-C did not adversely affect the metabolic activity of human chondrocytes.					
32408255	7	5	theme	healthy	1453:1459	arg1	0.07 ± 0.04					1440:1450	0.07 ± 0.04	1440:1450	0.07 ± 0.04	1440:1450	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	7	5	theme	healthy	1453:1459	arg1	tissue					1461:1466	healthy tissue	1453:1466	healthy tissue	1453:1466	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	1	6	from	present	184:190	arg1	body					236:239	the human body	226:239	the human body where they provide ultralow friction and maintain normal physiological function	226:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	7	7	theme	fluid	1534:1538	arg1	presence					1513:1520	the presence	1509:1520	the presence of synovial fluid	1509:1538	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	10	8	theme	new	1778:1780	arg1	insight					1786:1792	new key insight	1778:1792	new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules	1778:1883	This study provides new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules.					
32408255	4	9	theme	degraded	758:765	arg1	surface					777:783	degraded cartilage surface	758:783	degraded cartilage surface	758:783	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	1	10	theme	normal	291:296	arg1	function					312:319	normal physiological function	291:319	normal physiological function	291:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	0	11	theme	splendens	104:112	arg1	reestablishment					78:92	reestablishment	78:92	reestablishment of lamina splendens	78:112	A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens.					
32408255	1	12	theme	physiological	298:310	arg1	function					312:319	normal physiological function	291:319	normal physiological function	291:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	9	13	theme	human	1738:1742	arg1	chondrocytes					1744:1755	human chondrocytes	1738:1755	human chondrocytes	1738:1755	Chi-C did not adversely affect the metabolic activity of human chondrocytes.					
32408255	7	14	theme	Chondroitinase	1372:1385	arg1	ABC					1387:1389	Chondroitinase ABC	1372:1389	Chondroitinase ABC	1372:1389	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	3	15	theme	hyaluronic	525:534	arg1	HA					542:543	HA	542:543	HA	542:543	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	3	15	theme	hyaluronic	525:534	arg1	acid					536:539	hyaluronic acid	525:539	hyaluronic acid (HA) orally or via intra-articular injection	525:584	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	5	16	dep	mimic	923:927	arg1	known					973:977	known	973:977	known as synovial fluid conditioning films (SyCF)	973:1021	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	3	17	from	surface	641:647	arg1	absence					656:662	the absence	652:662	the absence of adhesive molecules	652:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	8	18	theme	cartilage	1599:1607	arg1	treatment					1578:1586	Chi-C treatment	1572:1586	Chi-C treatment of damaged cartilage	1572:1607	After Chi-C treatment of damaged cartilage, the COF fell to 0.06 ± 0.03, which is comparable to healthy cartilage.					
32408255	5	19	theme	nanoscale	1094:1102	arg1	properties					1115:1124	their nanoscale frictional properties	1088:1124	their nanoscale frictional properties	1088:1124	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	4	20	theme	chitosan	856:863	arg1	Chi-C					875:879	Chi-C	875:879	Chi-C	875:879	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	4	20	theme	chitosan	856:863	arg1	catechol					865:872	a bioinspired mucoadhesive biopolymer chitosan catechol	818:872	a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C)	818:880	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	6	21	theme	glycoprotein	1145:1156	arg1	recruitment					1165:1175	glycoprotein (PRG4) recruitment	1145:1175	glycoprotein (PRG4) recruitment by Chi-C	1145:1184	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	5	22	theme	lamina	946:951	arg1	splendens					953:961	lamina splendens	946:961	lamina splendens	946:961	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	1	23	theme	articulating	199:210	arg1	interfaces					212:221	all articulating interfaces	195:221	all articulating interfaces	195:221	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	24	located	present	184:190	arg1	interfaces					212:221	all articulating interfaces	195:221	all articulating interfaces	195:221	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	24	located	present	184:190	arg2	films					135:139	Adsorbed lubricious films	115:139	Adsorbed lubricious films composed of biomacromolecules	115:169	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	24	located	present	184:190	arg1	body					236:239	the human body	226:239	the human body where they provide ultralow friction and maintain normal physiological function	226:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	6	25	theme	nanoscale	1238:1246	arg1	lubrication					1248:1258	nanoscale lubrication	1238:1258	nanoscale lubrication	1238:1258	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	3	26	theme	poor	602:605	arg1	adsorption					607:616	the poor adsorption	598:616	the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules	598:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	8	27	theme	healthy	1662:1668	arg1	cartilage					1670:1678	healthy cartilage	1662:1678	healthy cartilage	1662:1678	After Chi-C treatment of damaged cartilage, the COF fell to 0.06 ± 0.03, which is comparable to healthy cartilage.					
32408255	4	28	dep	the	806:808	arg1	help					810:813	help	810:813	help	810:813	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	10	29	theme	muco-adhesive	1861:1873	arg1	molecules					1875:1883	muco-adhesive molecules	1861:1883	muco-adhesive molecules	1861:1883	This study provides new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules.					
32408255	6	30	from	0.03	1319:1322	arg1	coefficient					1294:1304	the friction coefficient	1281:1304	the friction coefficient from 0.06 to 0.03	1281:1322	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	5	31	theme	force	1051:1055	arg1	microscopy					1057:1066	colloidal probe atomic force microscopy	1028:1066	colloidal probe atomic force microscopy	1028:1066	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	5	32	theme	splendens	953:961	arg1	formation					933:941	the formation	929:941	the formation of lamina splendens	929:961	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	5	33	theme	crystal	890:896	arg1	microbalance					898:909	Quartz crystal microbalance	883:909	Quartz crystal microbalance	883:909	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	7	34	from	0.07 ± 0.04	1440:1450	arg1	coefficient					1405:1415	the coefficient	1401:1415	the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage)	1401:1504	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	10	35	theme	key	1782:1784	arg1	insight					1786:1792	new key insight	1778:1792	new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules	1778:1883	This study provides new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules.					
32408255	4	36	theme	mucoadhesive	832:843	arg1	Chi-C					875:879	Chi-C	875:879	Chi-C	875:879	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	4	36	theme	mucoadhesive	832:843	arg1	catechol					865:872	a bioinspired mucoadhesive biopolymer chitosan catechol	818:872	a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C)	818:880	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	1	37	theme	Adsorbed	115:122	arg1	films					135:139	Adsorbed lubricious films	115:139	Adsorbed lubricious films composed of biomacromolecules	115:169	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	2	38	theme	lamina	464:469	arg1	composition					481:491	lamina splendens composition	464:491	lamina splendens composition	464:491	Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition.					
32408255	2	39	theme	splendens	471:479	arg1	composition					481:491	lamina splendens composition	464:491	lamina splendens composition	464:491	Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition.					
32408255	6	40	theme	recruitment	1165:1175	arg1	evidence					1133:1140	Clear evidence	1127:1140	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C	1127:1184	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	3	41	theme	adhesive	667:674	arg1	molecules					676:684	adhesive molecules	667:684	adhesive molecules	667:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	1	42	theme	lubricious	124:133	arg1	films					135:139	Adsorbed lubricious films	115:139	Adsorbed lubricious films composed of biomacromolecules	115:169	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	43	theme	human	230:234	arg1	body					236:239	the human body	226:239	the human body where they provide ultralow friction and maintain normal physiological function	226:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	6	44	dep	0.03	1319:1322	arg1	to					1316:1317	to	1316:1317	to	1316:1317	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	3	45	theme	molecules	676:684	arg1	absence					656:662	the absence	652:662	the absence of adhesive molecules	652:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	4	46	theme	bioinspired	820:830	arg1	Chi-C					875:879	Chi-C	875:879	Chi-C	875:879	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	4	46	theme	bioinspired	820:830	arg1	catechol					865:872	a bioinspired mucoadhesive biopolymer chitosan catechol	818:872	a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C)	818:880	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	7	47	theme	tissue	1491:1496	arg1	damage					1498:1503	tissue damage	1491:1503	tissue damage	1491:1503	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	5	48	theme	Quartz	883:888	arg1	microbalance					898:909	Quartz crystal microbalance	883:909	Quartz crystal microbalance	883:909	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	1	49	dep	they	247:250	arg1	maintain					282:289	maintain	282:289	maintain normal physiological function	282:319	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	1	49	dep	they	247:250	arg1	provide					252:258	provide	252:258	provide ultralow friction	252:276	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	5	50	used	used	915:918	arg2	microbalance					898:909	Quartz crystal microbalance	883:909	Quartz crystal microbalance	883:909	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	1	51	from	body	236:239	arg1	present					184:190	present	184:190	present	184:190	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	2	52	theme	synovial	445:452	arg1	fluid					454:458	synovial fluid	445:458	synovial fluid	445:458	Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition.					
32408255	0	53	theme	cartilage	60:68	arg1	lubrication					36:46	lubrication	36:46	lubrication of degraded cartilage	36:68	A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens.					
32408255	5	54	used	used	1072:1075	arg2	microscopy					1057:1066	colloidal probe atomic force microscopy	1028:1066	colloidal probe atomic force microscopy	1028:1066	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	4	55	theme	biopolymer	845:854	arg1	Chi-C					875:879	Chi-C	875:879	Chi-C	875:879	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	4	55	theme	biopolymer	845:854	arg1	catechol					865:872	a bioinspired mucoadhesive biopolymer chitosan catechol	818:872	a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C)	818:880	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	0	56	theme	degraded	51:58	arg1	cartilage					60:68	degraded cartilage	51:68	degraded cartilage	51:68	A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens.					
32408255	7	57	dep	0.07 ± 0.04	1440:1450	arg1	0.15 ± 0.03					1472:1482	0.15 ± 0.03	1472:1482	0.15 ± 0.03	1472:1482	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	7	57	dep	0.07 ± 0.04	1440:1450	arg1	to					1469:1470	to	1469:1470	to	1469:1470	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	5	58	theme	frictional	1104:1113	arg1	properties					1115:1124	their nanoscale frictional properties	1088:1124	their nanoscale frictional properties	1088:1124	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	5	59	theme	colloidal	1028:1036	arg1	microscopy					1057:1066	colloidal probe atomic force microscopy	1028:1066	colloidal probe atomic force microscopy	1028:1066	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	3	60	theme	HA	621:622	arg1	adsorption					607:616	the poor adsorption	598:616	the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules	598:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	9	61	theme	chondrocytes	1744:1755	arg1	activity					1726:1733	the metabolic activity	1712:1733	the metabolic activity of human chondrocytes	1712:1755	Chi-C did not adversely affect the metabolic activity of human chondrocytes.					
32408255	6	62	theme	Clear	1127:1131	arg1	evidence					1133:1140	Clear evidence	1127:1140	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C	1127:1184	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	6	63	theme	SyCF	1212:1215	arg1	softness					1200:1207	the softness	1196:1207	the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03	1196:1322	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	4	64	from	lubrication	743:753	arg1	surface					777:783	degraded cartilage surface	758:783	degraded cartilage surface	758:783	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	5	65	theme	probe	1038:1042	arg1	microscopy					1057:1066	colloidal probe atomic force microscopy	1028:1066	colloidal probe atomic force microscopy	1028:1066	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	2	66	from	alterations	430:440	arg1	fluid					454:458	synovial fluid	445:458	synovial fluid	445:458	Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition.					
32408255	2	66	from	alterations	430:440	arg1	composition					481:491	lamina splendens composition	464:491	lamina splendens composition	464:491	Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition.					
32408255	4	67	theme	natural	735:741	arg1	lubrication					743:753	natural lubrication	735:753	natural lubrication on degraded cartilage surface	735:783	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	4	68	theme	cartilage	767:775	arg1	surface					777:783	degraded cartilage surface	758:783	degraded cartilage surface	758:783	Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C).					
32408255	5	69	theme	synovial	982:989	arg1	fluid					991:995	synovial fluid	982:995	synovial fluid conditioning films (SyCF)	982:1021	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	5	70	theme	atomic	1044:1049	arg1	microscopy					1057:1066	colloidal probe atomic force microscopy	1028:1066	colloidal probe atomic force microscopy	1028:1066	Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties.					
32408255	7	71	theme	cartilage	1344:1352	arg1	damage					1354:1359	cartilage damage	1344:1359	cartilage damage induced by Chondroitinase ABC	1344:1389	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	1	72	from	interfaces	212:221	arg1	present					184:190	present	184:190	present	184:190	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	3	73	theme	intra-articular	560:574	arg1	injection					576:584	intra-articular injection	560:584	intra-articular injection	560:584	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	6	74	theme	friction	1285:1292	arg1	coefficient					1294:1304	the friction coefficient	1281:1304	the friction coefficient from 0.06 to 0.03	1281:1322	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	7	75	theme	synovial	1525:1532	arg1	fluid					1534:1538	synovial fluid	1525:1538	synovial fluid	1525:1538	At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min.					
32408255	3	76	theme	pain	687:690	arg1	relief					692:697	pain relief	687:697	pain relief	687:697	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	3	77	theme	cartilage	631:639	arg1	surface					641:647	the cartilage surface	627:647	the cartilage surface in the absence of adhesive molecules	627:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	3	78	from	adsorption	607:616	arg1	surface					641:647	the cartilage surface	627:647	the cartilage surface in the absence of adhesive molecules	627:684	Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary.					
32408255	1	79	theme	ultralow	260:267	arg1	friction					269:276	ultralow friction	260:276	ultralow friction	260:276	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function.					
32408255	10	80	theme	molecules	1875:1883	arg1	use					1854:1856	the use	1850:1856	the use of muco-adhesive molecules	1850:1883	This study provides new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules.					
32408255	6	81	gly	glycoprotein	1145:1156	arg1	glycoprotein					1145:1156	glycoprotein (PRG4) recruitment	1145:1175	glycoprotein (PRG4) recruitment by Chi-C	1145:1184	Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03.					
32408255	2	82	theme	cartilage	400:408	arg1	damage					410:415	cartilage damage	400:415	cartilage damage	400:415	Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition.					
32408255	8	83	theme	damaged	1591:1597	arg1	cartilage					1599:1607	damaged cartilage	1591:1607	damaged cartilage	1591:1607	After Chi-C treatment of damaged cartilage, the COF fell to 0.06 ± 0.03, which is comparable to healthy cartilage.					
32408255	8	84	theme	Chi-C	1572:1576	arg1	treatment					1578:1586	Chi-C treatment	1572:1586	Chi-C treatment of damaged cartilage	1572:1607	After Chi-C treatment of damaged cartilage, the COF fell to 0.06 ± 0.03, which is comparable to healthy cartilage.					
32260563	5	0	from	effects	642:648	arg1	phenotype					735:743	the diet-induced obese phenotype	712:743	the diet-induced obese phenotype in high fat (HF)-fed mice	712:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	5	0	from	effects	642:648	arg1	axis					703:706	the microbiota-gut-brain axis	678:706	the microbiota-gut-brain axis	678:706	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	7	1	theme	General	1032:1038	arg1	fat					1080:1082	fat	1080:1082	fat	1080:1082	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	1	theme	General	1032:1038	arg1	mass					1093:1096	lean mass	1088:1096	lean mass	1088:1096	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	1	theme	General	1032:1038	arg1	phenotypes					1040:1049	General phenotypes	1032:1049	General phenotypes (body weight, energy intake, fat and lean mass)	1032:1097	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	1	theme	General	1032:1038	arg1	weight					1057:1062	body weight	1052:1062	body weight	1052:1062	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	1	theme	General	1032:1038	arg1	intake					1072:1077	energy intake	1065:1077	energy intake	1065:1077	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	9	2	theme	study	1604:1608	arg1	findings					1610:1617	our study findings	1600:1617	our study findings	1600:1617	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	5	3	theme	beneficial	631:640	arg1	effects					642:648	the beneficial effects	627:648	the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice	627:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	6	4	theme	weight-matched	850:863	arg1	groups					865:870	six weight-matched groups	846:870	six weight-matched groups	846:870	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	9	5	theme	%	1374:1374	arg1	2'-FL					1376:1380	10% 2'-FL	1372:1380	10% 2'-FL	1372:1380	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	6	6	theme	C57/BL6	777:783	arg1	mice					785:788	Male C57/BL6 mice	772:788	Male C57/BL6 mice (n = 6/group; six weeks old)	772:817	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	11	7	theme	Gene	1753:1756	arg1	expressions					1758:1768	Gene expressions	1753:1768	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum	1753:1852	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	5	8	from	axis	703:706	arg1	mice					766:769	high fat (HF)-fed mice	748:769	high fat (HF)-fed mice	748:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	7	9	theme	lipid	1200:1204	arg1	profiles					1206:1213	lipid profiles	1200:1213	lipid profiles	1200:1213	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	0	10	theme	2'-fucosyllactose	0:16	arg1	Supplementation					18:32	2'-fucosyllactose Supplementation	0:32	2'-fucosyllactose Supplementation	0:32	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	8	11	theme	diet-induced	1279:1290	arg1	increases					1292:1300	HF diet-induced increases	1276:1300	HF diet-induced increases in energy intake, fat mass and body weight gain	1276:1348	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	11	12	theme	macrophage	1804:1813	arg1	protein-1					1831:1839	macrophage chemoattractant protein-1	1804:1839	macrophage chemoattractant protein-1	1804:1839	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	13	13	theme	gut-brain	2253:2261	arg1	signaling					2263:2271	gut-brain signaling	2253:2271	gut-brain signaling in HF-fed mice	2253:2286	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	8	14	from	increases	1292:1300	arg1	intake					1312:1317	energy intake	1305:1317	energy intake	1305:1317	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	8	14	from	increases	1292:1300	arg1	mass					1324:1327	fat mass	1320:1327	fat mass	1320:1327	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	8	14	from	increases	1292:1300	arg1	gain					1345:1348	body weight gain	1333:1348	body weight gain	1333:1348	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	14	15	theme	HF	2372:2373	arg1	diets					2375:2379	HF diets	2372:2379	HF diets	2372:2379	These findings support the use of 2'-FL for modulating the hyperphagic response to HF diets and improving the microbiota-gut-brain axis.					
32260563	9	16	theme	cecal	1409:1413	arg1	microbiota					1415:1424	cecal microbiota	1409:1424	cecal microbiota	1409:1424	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	13	17	theme	%	2127:2127	arg1	2'-FL					2129:2133	10% 2'-FL	2125:2133	10% 2'-FL	2125:2133	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	10	18	theme	HF	1672:1673	arg1	impairment					1688:1697	the HF diet-induced impairment	1668:1697	the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake	1668:1750	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	0	19	theme	Fat-Fed	135:141	arg1	Mice					143:146	High Fat-Fed Mice	130:146	High Fat-Fed Mice	130:146	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	5	20	from	phenotype	735:743	arg1	mice					766:769	high fat (HF)-fed mice	748:769	high fat (HF)-fed mice	748:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	1	21	theme	impaired	220:227	arg1	signaling					237:245	impaired satiety signaling	220:245	impaired satiety signaling	220:245	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	13	22	from	profiles	2240:2247	arg1	mice					2283:2286	HF-fed mice	2276:2286	HF-fed mice	2276:2286	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	11	23	theme	IL	1786:1787	arg1	expressions					1758:1768	Gene expressions	1753:1768	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum	1753:1852	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	6	24	with	HF	920:921	arg1	HF_2'-FL					964:971	HF_2'-FL	964:971	HF_2'-FL	964:971	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	24	with	HF	920:921	arg1	2'-FL					957:961	2'-FL	957:961	2'-FL (HF_2'-FL)	957:972	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	25	dep	mice	785:788	arg1	6/group					795:801	n = 6/group	791:801	n = 6/group	791:801	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	26	dep	low-fat	889:895	arg1	%					904:904	LF; 10%	898:904	LF; 10% kcal as fat	898:916	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	7	27	theme	gut-brain	1134:1142	arg1	signaling					1144:1152	gut-brain signaling	1134:1152	gut-brain signaling	1134:1152	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	11	28	theme	IL-6	1794:1797	arg1	expressions					1758:1768	Gene expressions	1753:1768	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum	1753:1852	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	10	29	theme	cholecystokinin-induced	1702:1724	arg1	inhibition					1726:1735	cholecystokinin-induced inhibition	1702:1735	cholecystokinin-induced inhibition of food intake	1702:1750	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	5	30	theme	supplementation	659:673	arg1	effects					642:648	the beneficial effects	627:648	the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice	627:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	11	31	theme	chemoattractant	1815:1829	arg1	protein-1					1831:1839	macrophage chemoattractant protein-1	1804:1839	macrophage chemoattractant protein-1	1804:1839	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	9	32	theme	LF-	1454:1456	arg1	mice					1469:1472	LF- and HF-fed mice	1454:1472	LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings	1454:1617	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	12	33	theme	gamma	2043:2047	arg1	upregulation					1976:1987	HF diet-induced upregulation	1960:1987	HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis,	1960:2089	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	11	34	theme	protein-1	1831:1839	arg1	expressions					1758:1768	Gene expressions	1753:1768	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum	1753:1852	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	9	35	theme	HF-fed	1462:1467	arg1	mice					1469:1472	LF- and HF-fed mice	1454:1472	LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings	1454:1617	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	10	36	theme	intake	1745:1750	arg1	inhibition					1726:1735	cholecystokinin-induced inhibition	1702:1735	cholecystokinin-induced inhibition of food intake	1702:1750	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	5	37	theme	fat	753:755	arg1	mice					766:769	high fat (HF)-fed mice	748:769	high fat (HF)-fed mice	748:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	9	38	with	mice	1469:1472	arg1	increase					1482:1489	an increase	1479:1489	an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings	1479:1617	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	12	39	theme	hepatic	1992:1998	arg1	gamma					2043:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	12	39	theme	hepatic	1992:1998	arg1	factor					2066:2071	a transcription factor	2050:2071	a transcription factor for adipogenesis	2050:2088	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	12	40	theme	transcription	2052:2064	arg1	gamma					2043:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	12	40	theme	transcription	2052:2064	arg1	factor					2066:2071	a transcription factor	2050:2071	a transcription factor for adipogenesis	2050:2088	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	1	41	theme	fat	177:179	arg1	accumulation					181:192	fat accumulation	177:192	fat accumulation	177:192	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	12	42	theme	proliferator-activated	2011:2032	arg1	gamma					2043:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	12	42	theme	proliferator-activated	2011:2032	arg1	factor					2066:2071	a transcription factor	2050:2071	a transcription factor for adipogenesis	2050:2088	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	0	43	theme	Gut	112:114	arg1	Microbiota					116:125	the Gut Microbiota	108:125	the Gut Microbiota in High Fat-Fed Mice	108:146	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	13	44	theme	compositional	2142:2154	arg1	changes					2156:2162	compositional changes	2142:2162	compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice	2142:2286	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	6	45	theme	kcal	906:909	arg1	%					904:904	LF; 10%	898:904	LF; 10% kcal as fat	898:916	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	9	46	theme	Parabacteroides	1494:1508	arg1	abundance					1510:1518	Parabacteroides abundance	1494:1518	Parabacteroides abundance	1494:1518	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	12	47	theme	HF	1960:1961	arg1	upregulation					1976:1987	HF diet-induced upregulation	1960:1987	HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis,	1960:2089	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	13	48	theme	metabolic	2230:2238	arg1	profiles					2240:2247	metabolic profiles	2230:2247	metabolic profiles	2230:2247	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	6	49	theme	HF	944:945	arg1	diet					947:950	HF diet	944:950	HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks	944:1029	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	11	50	theme	%	1892:1892	arg1	2'-FL					1894:1898	10% 2'-FL	1890:1898	10% 2'-FL	1890:1898	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	3	51	theme	human	444:448	arg1	milk					450:453	human milk	444:453	human milk	444:453	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	4	52	from	effects	559:565	arg1	obesity					579:585	obesity	579:585	obesity	579:585	However, little is known about the beneficial effects of 2'-FL in obesity.					
32260563	1	53	theme	gut	276:278	arg1	dysbiosis					290:298	gut microbial dysbiosis	276:298	gut microbial dysbiosis	276:298	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	7	54	dep	phenotypes	1040:1049	arg1	phenotypes					1040:1049	General phenotypes	1032:1049	General phenotypes (body weight, energy intake, fat and lean mass)	1032:1097	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	54	dep	phenotypes	1040:1049	arg1	mass					1093:1096	lean mass	1088:1096	lean mass	1088:1096	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	54	dep	phenotypes	1040:1049	arg1	fat					1080:1082	fat	1080:1082	fat	1080:1082	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	54	dep	phenotypes	1040:1049	arg1	weight					1057:1062	body weight	1052:1062	body weight	1052:1062	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	54	dep	phenotypes	1040:1049	arg1	intake					1072:1077	energy intake	1065:1077	energy intake	1065:1077	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	3	55	located	found	435:439	arg2	oligosaccharide					419:433	The predominant oligosaccharide	403:433	The predominant oligosaccharide	403:433	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	3	55	located	found	435:439	arg2	prebiotic					466:474	a prebiotic	464:474	a prebiotic with beneficial effects on the host	464:510	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	3	55	located	found	435:439	arg1	milk					450:453	human milk	444:453	human milk	444:453	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	8	56	theme	HF	1276:1277	arg1	increases					1292:1300	HF diet-induced increases	1276:1300	HF diet-induced increases in energy intake, fat mass and body weight gain	1276:1348	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	7	57	theme	lean	1088:1091	arg1	phenotypes					1040:1049	General phenotypes	1032:1049	General phenotypes (body weight, energy intake, fat and lean mass)	1032:1097	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	57	theme	lean	1088:1091	arg1	mass					1093:1096	lean mass	1088:1096	lean mass	1088:1096	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	6	58	dep	6/group	795:801	arg1	weeks					808:812	six weeks	804:812	six weeks old	804:816	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	5	59	theme	-fed	761:764	arg1	mice					766:769	high fat (HF)-fed mice	748:769	high fat (HF)-fed mice	748:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	8	60	dep	%	1237:1237	arg1	10					1235:1236	10	1235:1236	10	1235:1236	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	6	61	with	diet	947:950	arg1	HF_2'-FL					964:971	HF_2'-FL	964:971	HF_2'-FL	964:971	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	61	with	diet	947:950	arg1	2'-FL					957:961	2'-FL	957:961	2'-FL (HF_2'-FL)	957:972	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	3	62	theme	predominant	407:417	arg1	oligosaccharide					419:433	The predominant oligosaccharide	403:433	The predominant oligosaccharide	403:433	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	3	62	theme	predominant	407:417	arg1	prebiotic					466:474	a prebiotic	464:474	a prebiotic with beneficial effects on the host	464:510	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	8	63	theme	energy	1305:1310	arg1	intake					1312:1317	energy intake	1305:1317	energy intake	1305:1317	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	2	64	theme	metabolites	345:355	arg1	targets					372:378	attractive targets	361:378	attractive targets for obesity treatment	361:400	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	2	64	theme	metabolites	345:355	arg1	Manipulations					301:313	Manipulations	301:313	Manipulations of the gut microbiota and its metabolites	301:355	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	0	65	theme	Diet-Induced	67:78	arg1	Phenotype					86:94	Diet-Induced Obese Phenotype	67:94	Diet-Induced Obese Phenotype	67:94	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	9	66	from	increase	1482:1489	arg1	lactate					1524:1530	lactate	1524:1530	lactate	1524:1530	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	9	66	from	increase	1482:1489	arg1	abundance					1510:1518	Parabacteroides abundance	1494:1518	Parabacteroides abundance	1494:1518	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	9	67	theme	metabolic	1566:1574	arg1	effects					1576:1582	metabolic effects	1566:1582	metabolic effects	1566:1582	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	3	68	theme	beneficial	481:490	arg1	effects					492:498	beneficial effects	481:498	beneficial effects on the host	481:510	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	6	69	theme	=	793:793	arg1	6/group					795:801	n = 6/group	791:801	n = 6/group	791:801	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	10	70	theme	food	1740:1743	arg1	intake					1745:1750	food intake	1740:1750	food intake	1740:1750	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	7	71	theme	body	1052:1055	arg1	phenotypes					1040:1049	General phenotypes	1032:1049	General phenotypes (body weight, energy intake, fat and lean mass)	1032:1097	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	71	theme	body	1052:1055	arg1	weight					1057:1062	body weight	1052:1062	body weight	1052:1062	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	8	72	theme	body	1333:1336	arg1	gain					1345:1348	body weight gain	1333:1348	body weight gain	1333:1348	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	2	73	theme	attractive	361:370	arg1	targets					372:378	attractive targets	361:378	attractive targets for obesity treatment	361:400	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	2	73	theme	attractive	361:370	arg1	Manipulations					301:313	Manipulations	301:313	Manipulations of the gut microbiota and its metabolites	301:355	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	9	74	theme	10	1372:1373	arg1	%					1374:1374	%	1374:1374	%	1374:1374	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	4	75	theme	2'-FL	570:574	arg1	effects					559:565	the beneficial effects	544:565	the beneficial effects of 2'-FL in obesity	544:585	However, little is known about the beneficial effects of 2'-FL in obesity.					
32260563	13	76	theme	HF-fed	2276:2281	arg1	mice					2283:2286	HF-fed mice	2276:2286	HF-fed mice	2276:2286	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	11	77	theme	interleukin	1773:1783	arg1	IL					1786:1787	interleukin (IL)-1β	1773:1791	interleukin (IL)-1β	1773:1791	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	9	78	theme	2'-FL	1376:1380	arg1	supplementation					1353:1367	A supplementation	1351:1367	A supplementation of 10% 2'-FL	1351:1380	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	12	79	theme	gene	2098:2101	arg1	level					2103:2107	the gene level	2094:2107	the gene level	2094:2107	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	5	80	theme	study	604:608	arg1	aim					592:594	The aim	588:594	The aim of this study	588:608	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	6	81	theme	Male	772:775	arg1	mice					785:788	Male C57/BL6 mice	772:788	Male C57/BL6 mice (n = 6/group; six weeks old)	772:817	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	10	82	theme	%	1637:1637	arg1	2'-FL					1639:1643	10% 2'-FL	1635:1643	10% 2'-FL	1635:1643	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	3	83	from	effects	492:498	arg1	host					507:510	the host	503:510	the host	503:510	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	2	84	theme	gut	322:324	arg1	microbiota					326:335	the gut microbiota	318:335	the gut microbiota	318:335	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	1	85	theme	chronic	195:201	arg1	inflammation					203:214	chronic inflammation	195:214	chronic inflammation	195:214	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	10	86	theme	diet-induced	1675:1686	arg1	impairment					1688:1697	the HF diet-induced impairment	1668:1697	the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake	1668:1750	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	1	87	from	part	268:271	arg1	due					261:263	due	261:263	due	261:263	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	9	88	theme	microbiota	1415:1424	arg1	composition					1394:1404	the composition	1390:1404	the composition of cecal microbiota and metabolites	1390:1440	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	6	89	with	low-fat	889:895	arg1	HF_2'-FL					964:971	HF_2'-FL	964:971	HF_2'-FL	964:971	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	89	with	low-fat	889:895	arg1	2'-FL					957:961	2'-FL	957:961	2'-FL (HF_2'-FL)	957:972	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	3	90	with	prebiotic	466:474	arg1	effects					492:498	beneficial effects	481:498	beneficial effects on the host	481:510	The predominant oligosaccharide found in human milk, acts as a prebiotic with beneficial effects on the host.					
32260563	1	91	theme	satiety	229:235	arg1	signaling					237:245	impaired satiety signaling	220:245	impaired satiety signaling	220:245	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	11	92	from	expressions	1758:1768	arg1	cecum					1848:1852	the cecum	1844:1852	the cecum	1844:1852	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	14	93	theme	hyperphagic	2348:2358	arg1	response					2360:2367	the hyperphagic response	2344:2367	the hyperphagic response to HF diets	2344:2379	These findings support the use of 2'-FL for modulating the hyperphagic response to HF diets and improving the microbiota-gut-brain axis.					
32260563	9	94	theme	metabolites	1430:1440	arg1	composition					1394:1404	the composition	1390:1404	the composition of cecal microbiota and metabolites	1390:1440	A supplementation of 10% 2'-FL changed the composition of cecal microbiota and metabolites compared to LF- and HF-fed mice with an increase in Parabacteroides abundance and lactate and pyruvate, respectively, whose metabolic effects corresponded to our study findings.					
32260563	7	95	theme	intestinal	1155:1164	arg1	permeability					1166:1177	intestinal permeability	1155:1177	intestinal permeability	1155:1177	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	10	96	theme	inhibition	1726:1735	arg1	impairment					1688:1697	the HF diet-induced impairment	1668:1697	the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake	1668:1750	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32260563	5	97	theme	2'-FL	653:657	arg1	supplementation					659:673	2'-FL supplementation	653:673	2'-FL supplementation	653:673	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	13	98	from	signaling	2263:2271	arg1	mice					2283:2286	HF-fed mice	2276:2286	HF-fed mice	2276:2286	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	5	99	theme	microbiota-gut-brain	682:701	arg1	axis					703:706	the microbiota-gut-brain axis	678:706	the microbiota-gut-brain axis	678:706	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	14	100	theme	2'-FL	2323:2327	arg1	use					2316:2318	the use	2312:2318	the use of 2'-FL for modulating the hyperphagic response to HF diets and improving the microbiota-gut-brain axis	2312:2423	These findings support the use of 2'-FL for modulating the hyperphagic response to HF diets and improving the microbiota-gut-brain axis.					
32260563	0	101	from	Microbiota	116:125	arg1	Mice					143:146	High Fat-Fed Mice	130:146	High Fat-Fed Mice	130:146	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	5	102	theme	high	748:751	arg1	fat					753:755	high fat	748:755	high fat (HF)-fed mice	748:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	5	102	theme	high	748:751	arg1	HF					758:759	HF	758:759	HF	758:759	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	13	103	theme	gut	2167:2169	arg1	microbiota					2171:2180	gut microbiota	2167:2180	gut microbiota	2167:2180	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	12	104	theme	peroxisome	2000:2009	arg1	gamma					2043:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	12	104	theme	peroxisome	2000:2009	arg1	factor					2066:2071	a transcription factor	2050:2071	a transcription factor for adipogenesis	2050:2088	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	13	105	from	changes	2156:2162	arg1	metabolites					2186:2196	metabolites	2186:2196	metabolites	2186:2196	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	13	105	from	changes	2156:2162	arg1	microbiota					2171:2180	gut microbiota	2167:2180	gut microbiota	2167:2180	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	5	106	theme	obese	729:733	arg1	phenotype					735:743	the diet-induced obese phenotype	712:743	the diet-induced obese phenotype in high fat (HF)-fed mice	712:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	12	107	theme	diet-induced	1963:1974	arg1	upregulation					1976:1987	HF diet-induced upregulation	1960:1987	HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis,	1960:2089	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	13	108	from	improvements	2214:2225	arg1	profiles					2240:2247	metabolic profiles	2230:2247	metabolic profiles	2230:2247	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	13	108	from	improvements	2214:2225	arg1	signaling					2263:2271	gut-brain signaling	2253:2271	gut-brain signaling in HF-fed mice	2253:2286	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	12	109	theme	receptor	2034:2041	arg1	gamma					2043:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	hepatic peroxisome proliferator-activated receptor gamma	1992:2047	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	12	109	theme	receptor	2034:2041	arg1	factor					2066:2071	a transcription factor	2050:2071	a transcription factor for adipogenesis	2050:2088	Furthermore, 10% 2'-FL suppressed HF diet-induced upregulation of hepatic peroxisome proliferator-activated receptor gamma, a transcription factor for adipogenesis, at the gene level.					
32260563	5	110	theme	diet-induced	716:727	arg1	phenotype					735:743	the diet-induced obese phenotype	712:743	the diet-induced obese phenotype in high fat (HF)-fed mice	712:769	The aim of this study was to determine the beneficial effects of 2'-FL supplementation on the microbiota-gut-brain axis and the diet-induced obese phenotype in high fat (HF)-fed mice.					
32260563	6	111	dep	HF	920:921	arg1	%					926:926	45%	924:926	45% kcal as fat	924:938	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	112	from	%	991:991	arg1	low-fat					889:895	a low-fat	887:895	a low-fat (LF; 10% kcal as fat)	887:917	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	112	from	%	991:991	arg1	HF					920:921	HF	920:921	HF (45% kcal as fat)	920:939	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	112	from	%	991:991	arg1	diet					947:950	HF diet	944:950	HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks	944:1029	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	0	113	theme	High	130:133	arg1	Mice					143:146	High Fat-Fed Mice	130:146	High Fat-Fed Mice	130:146	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	13	114	theme	10	2125:2126	arg1	%					2127:2127	%	2127:2127	%	2127:2127	In conclusion, 10% 2'-FL led to compositional changes in gut microbiota and metabolites associated with improvements in metabolic profiles and gut-brain signaling in HF-fed mice.					
32260563	1	115	from	due	261:263	arg1	part					268:271	part	268:271	part	268:271	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	4	116	theme	beneficial	548:557	arg1	effects					559:565	the beneficial effects	544:565	the beneficial effects of 2'-FL in obesity	544:585	However, little is known about the beneficial effects of 2'-FL in obesity.					
32260563	2	117	theme	obesity	384:390	arg1	treatment					392:400	obesity treatment	384:400	obesity treatment	384:400	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	0	118	theme	Gut-Brain	43:51	arg1	Signaling					53:61	Gut-Brain Signaling	43:61	Gut-Brain Signaling	43:61	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	1	119	theme	microbial	280:288	arg1	dysbiosis					290:298	gut microbial dysbiosis	276:298	gut microbial dysbiosis	276:298	Obesity is characterized by fat accumulation, chronic inflammation and impaired satiety signaling, which may be due in part to gut microbial dysbiosis.					
32260563	11	120	theme	10	1890:1891	arg1	%					1892:1892	%	1892:1892	%	1892:1892	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	8	121	dep	2'-FL	1239:1243	arg1	%					1237:1237	%	1237:1237	%	1237:1237	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	6	122	theme	old	814:816	arg1	weeks					808:812	six weeks	804:812	six weeks old	804:816	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	6	123	theme	kcal	928:931	arg1	%					926:926	45%	924:926	45% kcal as fat	924:938	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	11	124	theme	HF	1916:1917	arg1	group					1919:1923	the HF group	1912:1923	the HF group	1912:1923	Gene expressions of interleukin (IL)-1β, IL-6, and macrophage chemoattractant protein-1 in the cecum were significantly downregulated by 10% 2'-FL compared to the HF group.					
32260563	0	125	theme	Obese	80:84	arg1	Phenotype					86:94	Diet-Induced Obese Phenotype	67:94	Diet-Induced Obese Phenotype	67:94	2'-fucosyllactose Supplementation Improves Gut-Brain Signaling and Diet-Induced Obese Phenotype and Changes the Gut Microbiota in High Fat-Fed Mice.					
32260563	7	126	theme	energy	1065:1070	arg1	phenotypes					1040:1049	General phenotypes	1032:1049	General phenotypes (body weight, energy intake, fat and lean mass)	1032:1097	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	7	126	theme	energy	1065:1070	arg1	intake					1072:1077	energy intake	1065:1077	energy intake	1065:1077	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	8	127	theme	fat	1320:1322	arg1	mass					1324:1327	fat mass	1320:1327	fat mass	1320:1327	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	2	128	theme	microbiota	326:335	arg1	targets					372:378	attractive targets	361:378	attractive targets for obesity treatment	361:400	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	2	128	theme	microbiota	326:335	arg1	Manipulations					301:313	Manipulations	301:313	Manipulations of the gut microbiota and its metabolites	301:355	Manipulations of the gut microbiota and its metabolites are attractive targets for obesity treatment.					
32260563	6	129	theme	n	791:791	arg1	6/group					795:801	n = 6/group	791:801	n = 6/group	791:801	Male C57/BL6 mice (n = 6/group; six weeks old) were counter-balanced into six weight-matched groups and fed either a low-fat (LF; 10% kcal as fat), HF (45% kcal as fat) or HF diet with 2'-FL (HF_2'-FL) at 1, 2, 5 and 10% (w/v) in drinking water for six weeks.					
32260563	14	130	theme	microbiota-gut-brain	2399:2418	arg1	axis					2420:2423	the microbiota-gut-brain axis	2395:2423	the microbiota-gut-brain axis	2395:2423	These findings support the use of 2'-FL for modulating the hyperphagic response to HF diets and improving the microbiota-gut-brain axis.					
32260563	7	131	theme	cecal	1100:1104	arg1	microbiome					1106:1115	cecal microbiome	1100:1115	cecal microbiome	1100:1115	General phenotypes (body weight, energy intake, fat and lean mass), cecal microbiome and metabolites, gut-brain signaling, intestinal permeability and inflammatory and lipid profiles were assessed.					
32260563	8	132	theme	weight	1338:1343	arg1	gain					1345:1348	body weight gain	1333:1348	body weight gain	1333:1348	Only 10% 2'-FL, but not 1, 2 or 5%, decreased HF diet-induced increases in energy intake, fat mass and body weight gain.					
32260563	10	133	theme	10	1635:1636	arg1	%					1637:1637	%	1637:1637	%	1637:1637	In particular, 10% 2'-FL significantly reversed the HF diet-induced impairment of cholecystokinin-induced inhibition of food intake.					
32633505	7	0	theme	renewable	1303:1311	arg1	bioproducts					1313:1323	advanced renewable bioproducts	1294:1323	advanced renewable bioproducts	1294:1323	Overall, this study has elucidated some of the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts.					
32633505	0	1	theme	Thermodynamic	79:91	arg1	Properties					118:127	Its Thermodynamic, Kinetic, and Electronic Properties	75:127	Its Thermodynamic, Kinetic, and Electronic Properties	75:127	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	3	2	theme	monomers	614:621	arg1	studies					530:536	Thermodynamic and kinetic studies	504:536	Thermodynamic and kinetic studies of the reaction pathways	504:561	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	3	2	theme	monomers	614:621	arg1	properties					582:591	the electronic properties	567:591	the electronic properties of the cellulose and monomers	567:621	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	1	3	theme	copolymerization	162:177	arg1	reaction					179:186	a copolymerization reaction	160:186	a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM)	160:264	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	5	4	theme	MBA	1009:1011	arg1	order					988:992	the decreasing order	973:992	the decreasing order of AM, AA, and MBA	973:1011	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	2	5	theme	7	472:472	arg1	%					473:473	%	473:473	%	473:473	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	6	6	with	kinetics	1065:1072	arg1	AM					1086:1087	AM	1086:1087	AM	1086:1087	The kinetic study also indicates that the reaction kinetics of CNF with AM is faster than with AA which is much faster than with MBA.					
32633505	4	7	theme	covalent	851:858	arg1	bonds					860:864	the homolytic covalent bonds C6-OH and C3-OH	837:880	the homolytic covalent bonds C6-OH and C3-OH	837:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	7	theme	covalent	851:858	arg1	C3-OH					876:880	C3-OH	876:880	C3-OH	876:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	7	theme	covalent	851:858	arg1	C6-OH					866:870	C6-OH	866:870	C6-OH	866:870	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	2	8	with	swelling	437:444	arg1	CNF					475:477	7% CNF	472:477	7% CNF about 200 g/g for water	472:501	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	5	9	theme	CNF	921:923	arg1	reaction					909:916	the reaction	905:916	the reaction of CNF with monomers	905:937	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	5	9	theme	CNF	921:923	arg1	favorable					960:968	favorable	960:968	favorable	960:968	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	3	10	dep	cellulose	600:608	arg1	the					596:598	the	596:598	the	596:598	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	0	11	theme	Kinetic	94:100	arg1	Properties					118:127	Its Thermodynamic, Kinetic, and Electronic Properties	75:127	Its Thermodynamic, Kinetic, and Electronic Properties	75:127	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	3	12	theme	theory	668:673	arg1	calculations					675:686	density functional theory calculations	649:686	density functional theory calculations	649:686	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	4	13	theme	homolytic	841:849	arg1	bonds					860:864	the homolytic covalent bonds C6-OH and C3-OH	837:880	the homolytic covalent bonds C6-OH and C3-OH	837:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	13	theme	homolytic	841:849	arg1	C3-OH					876:880	C3-OH	876:880	C3-OH	876:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	13	theme	homolytic	841:849	arg1	C6-OH					866:870	C6-OH	866:870	C6-OH	866:870	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	0	14	theme	Electronic	107:116	arg1	Properties					118:127	Its Thermodynamic, Kinetic, and Electronic Properties	75:127	Its Thermodynamic, Kinetic, and Electronic Properties	75:127	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	6	15	theme	kinetic	1018:1024	arg1	study					1026:1030	The kinetic study	1014:1030	The kinetic study	1014:1030	The kinetic study also indicates that the reaction kinetics of CNF with AM is faster than with AA which is much faster than with MBA.					
32633505	2	16	theme	g/g	459:461	arg1	swelling					437:444	swelling	437:444	swelling of about 250 g/g and with 7% CNF about 200 g/g for water	437:501	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	1	17	theme	absorption	333:342	arg1	performance					344:354	their absorption performance	327:354	their absorption performance as a function of composition	327:383	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	4	18	theme	radical	736:742	arg1	formation					744:752	the radical formation	732:752	the radical formation of cellulose that initiates the hydrogel process	732:801	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	5	19	with	reaction	909:916	arg1	monomers					930:937	monomers	930:937	monomers	930:937	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	3	20	theme	functional	657:666	arg1	calculations					675:686	density functional theory calculations	649:686	density functional theory calculations	649:686	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	1	21	with	N	270:270	arg1	AM					262:263	AM	262:263	AM	262:263	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	21	with	N	270:270	arg1	AA					242:243	AA	242:243	AA	242:243	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	21	with	N	270:270	arg1	acrylamide					250:259	acrylamide	250:259	acrylamide (AM)	250:264	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	21	with	N	270:270	arg1	acid					236:239	acrylic acid	228:239	acrylic acid (AA)	228:244	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	4	22	theme	Thermodynamic	689:701	arg1	investigations					703:716	Thermodynamic investigations	689:716	Thermodynamic investigations	689:716	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	5	23	from	favorable	960:968	arg1	order					988:992	the decreasing order	973:992	the decreasing order of AM, AA, and MBA	973:1011	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	1	24	theme	nanofibrillated	191:205	arg1	CNF					218:220	CNF	218:220	CNF	218:220	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	24	theme	nanofibrillated	191:205	arg1	cellulose					207:215	nanofibrillated cellulose	191:215	nanofibrillated cellulose (CNF)	191:221	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	3	25	theme	cellulose	600:608	arg1	studies					530:536	Thermodynamic and kinetic studies	504:536	Thermodynamic and kinetic studies of the reaction pathways	504:561	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	3	25	theme	cellulose	600:608	arg1	properties					582:591	the electronic properties	567:591	the electronic properties of the cellulose and monomers	567:621	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	1	26	theme	cellulose	207:215	arg1	N-methylene-bis-acrylamide					272:297	N-methylene-bis-acrylamide	272:297	N-methylene-bis-acrylamide (MBA)	272:303	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	26	theme	cellulose	207:215	arg1	reaction					179:186	a copolymerization reaction	160:186	a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM)	160:264	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	26	theme	cellulose	207:215	arg1	N					270:270	N	270:270	N	270:270	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	0	27	theme	Nanofibrillated	21:35	arg1	Hydrogel					53:60	Grafted Nanofibrillated Cellulose-Based Hydrogel	13:60	Grafted Nanofibrillated Cellulose-Based Hydrogel	13:60	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	2	28	contain	had	433:435	arg2	swelling					437:444	swelling	437:444	swelling of about 250 g/g and with 7% CNF about 200 g/g for water	437:501	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	2	28	contain	had	433:435	arg1	Hydrogels					401:409	Hydrogels	401:409	Hydrogels with 4% by weight CNF	401:431	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	4	29	theme	hydrogel	786:793	arg1	process					795:801	the hydrogel process	782:801	the hydrogel process	782:801	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	7	30	theme	chemical	1199:1206	arg1	characteristics					1208:1222	the key chemical characteristics	1191:1222	the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts	1191:1323	Overall, this study has elucidated some of the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts.					
32633505	0	31	theme	Grafted	13:19	arg1	Hydrogel					53:60	Grafted Nanofibrillated Cellulose-Based Hydrogel	13:60	Grafted Nanofibrillated Cellulose-Based Hydrogel	13:60	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	0	32	theme	Properties	118:127	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel	0:60	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	0	32	theme	Properties	118:127	arg1	Study					66:70	Study	66:70	Study of Its Thermodynamic, Kinetic, and Electronic Properties	66:127	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	3	33	theme	electronic	571:580	arg1	properties					582:591	the electronic properties	567:591	the electronic properties of the cellulose and monomers	567:621	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	6	34	theme	CNF	1077:1079	arg1	kinetics					1065:1072	the reaction kinetics	1052:1072	the reaction kinetics of CNF with AM	1052:1087	The kinetic study also indicates that the reaction kinetics of CNF with AM is faster than with AA which is much faster than with MBA.					
32633505	6	34	theme	CNF	1077:1079	arg1	faster					1092:1097	faster	1092:1097	faster	1092:1097	The kinetic study also indicates that the reaction kinetics of CNF with AM is faster than with AA which is much faster than with MBA.					
32633505	0	35	theme	Hydrogel	53:60	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel	0:60	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	0	35	theme	Hydrogel	53:60	arg1	Study					66:70	Study	66:70	Study of Its Thermodynamic, Kinetic, and Electronic Properties	66:127	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	3	36	theme	kinetic	522:528	arg1	studies					530:536	Thermodynamic and kinetic studies	504:536	Thermodynamic and kinetic studies of the reaction pathways	504:561	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	5	37	from	order	988:992	arg1	reaction					909:916	the reaction	905:916	the reaction of CNF with monomers	905:937	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	5	37	from	order	988:992	arg1	favorable					960:968	favorable	960:968	favorable	960:968	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	0	38	theme	Cellulose-Based	37:51	arg1	Hydrogel					53:60	Grafted Nanofibrillated Cellulose-Based Hydrogel	13:60	Grafted Nanofibrillated Cellulose-Based Hydrogel	13:60	Synthesis of Grafted Nanofibrillated Cellulose-Based Hydrogel and Study of Its Thermodynamic, Kinetic, and Electronic Properties.					
32633505	2	39	theme	%	473:473	arg1	CNF					475:477	7% CNF	472:477	7% CNF about 200 g/g for water	472:501	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	7	40	theme	advanced	1294:1301	arg1	bioproducts					1313:1323	advanced renewable bioproducts	1294:1323	advanced renewable bioproducts	1294:1323	Overall, this study has elucidated some of the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts.					
32633505	5	41	theme	decreasing	977:986	arg1	order					988:992	the decreasing order	973:992	the decreasing order of AM, AA, and MBA	973:1011	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	2	42	theme	weight	422:427	arg1	CNF					429:431	weight CNF	422:431	weight CNF	422:431	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	2	43	dep	CNF	475:477	arg1	g/g					489:491	about 200 g/g	479:491	7% CNF about 200 g/g for water	472:501	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	5	44	theme	AA	1001:1002	arg1	order					988:992	the decreasing order	973:992	the decreasing order of AM, AA, and MBA	973:1011	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	3	45	theme	Thermodynamic	504:516	arg1	studies					530:536	Thermodynamic and kinetic studies	504:536	Thermodynamic and kinetic studies of the reaction pathways	504:561	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	2	46	with	Hydrogels	401:409	arg1	%					417:417	4%	416:417	4% by weight CNF	416:431	Hydrogels with 4% by weight CNF had swelling of about 250 g/g and with 7% CNF about 200 g/g for water.					
32633505	4	47	dep	bonds	860:864	arg1	bonds					860:864	the homolytic covalent bonds C6-OH and C3-OH	837:880	the homolytic covalent bonds C6-OH and C3-OH	837:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	47	dep	bonds	860:864	arg1	C3-OH					876:880	C3-OH	876:880	C3-OH	876:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	47	dep	bonds	860:864	arg1	C6-OH					866:870	C6-OH	866:870	C6-OH	866:870	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	4	48	theme	bonds	860:864	arg1	breaking					825:832	the breaking	821:832	the breaking of the homolytic covalent bonds C6-OH and C3-OH	821:880	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	1	49	theme	composition	373:383	arg1	function					361:368	a function	359:368	a function of composition	359:383	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	5	50	theme	AM	997:998	arg1	order					988:992	the decreasing order	973:992	the decreasing order of AM, AA, and MBA	973:1011	The results show that the reaction of CNF with monomers is thermodynamically favorable in the decreasing order of AM, AA, and MBA.					
32633505	1	51	with	reaction	179:186	arg1	AM					262:263	AM	262:263	AM	262:263	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	51	with	reaction	179:186	arg1	AA					242:243	AA	242:243	AA	242:243	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	51	with	reaction	179:186	arg1	acrylamide					250:259	acrylamide	250:259	acrylamide (AM)	250:264	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	51	with	reaction	179:186	arg1	acid					236:239	acrylic acid	228:239	acrylic acid (AA)	228:244	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	4	52	theme	cellulose	757:765	arg1	formation					744:752	the radical formation	732:752	the radical formation of cellulose that initiates the hydrogel process	732:801	Thermodynamic investigations revealed that the radical formation of cellulose that initiates the hydrogel process can occur through the breaking of the homolytic covalent bonds C6-OH and C3-OH.					
32633505	3	53	theme	reaction	545:552	arg1	pathways					554:561	the reaction pathways	541:561	the reaction pathways	541:561	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	6	54	theme	reaction	1056:1063	arg1	kinetics					1065:1072	the reaction kinetics	1052:1072	the reaction kinetics of CNF with AM	1052:1087	The kinetic study also indicates that the reaction kinetics of CNF with AM is faster than with AA which is much faster than with MBA.					
32633505	6	54	theme	reaction	1056:1063	arg1	faster					1092:1097	faster	1092:1097	faster	1092:1097	The kinetic study also indicates that the reaction kinetics of CNF with AM is faster than with AA which is much faster than with MBA.					
32633505	1	55	theme	acrylic	228:234	arg1	AA					242:243	AA	242:243	AA	242:243	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	1	55	theme	acrylic	228:234	arg1	acid					236:239	acrylic acid	228:239	acrylic acid (AA)	228:244	Hydrogels were synthesized by a copolymerization reaction of nanofibrillated cellulose (CNF) with acrylic acid (AA) and acrylamide (AM) and N,N-methylene-bis-acrylamide (MBA) as a cross-linker and their absorption performance as a function of composition was determined.					
32633505	3	56	theme	pathways	554:561	arg1	studies					530:536	Thermodynamic and kinetic studies	504:536	Thermodynamic and kinetic studies of the reaction pathways	504:561	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	3	56	theme	pathways	554:561	arg1	properties					582:591	the electronic properties	567:591	the electronic properties of the cellulose and monomers	567:621	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
32633505	7	57	theme	key	1195:1197	arg1	characteristics					1208:1222	the key chemical characteristics	1191:1222	the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts	1191:1323	Overall, this study has elucidated some of the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts.					
32633505	7	58	theme	structures	1272:1281	arg1	derivatization					1240:1253	the derivatization	1236:1253	the derivatization of nanocellulose structures to produce advanced renewable bioproducts	1236:1323	Overall, this study has elucidated some of the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts.					
32633505	7	59	theme	nanocellulose	1258:1270	arg1	structures					1272:1281	nanocellulose structures	1258:1281	nanocellulose structures	1258:1281	Overall, this study has elucidated some of the key chemical characteristics that impact the derivatization of nanocellulose structures to produce advanced renewable bioproducts.					
32633505	3	60	theme	density	649:655	arg1	calculations					675:686	density functional theory calculations	649:686	density functional theory calculations	649:686	Thermodynamic and kinetic studies of the reaction pathways and the electronic properties of the cellulose and monomers were investigated through density functional theory calculations.					
34714122	5	0	theme	experimental	762:773	arg1	strain					789:794	the experimental gut microbial strain	758:794	the experimental gut microbial strain	758:794	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	9	1	theme	cryoprotectants	1608:1622	arg1	importance					1586:1595	the importance	1582:1595	the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens	1582:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	5	2	theme	gut	775:777	arg1	strain					789:794	the experimental gut microbial strain	758:794	the experimental gut microbial strain	758:794	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	7	3	from	situations	1301:1310	arg1	process					1319:1325	the process	1315:1325	the process of distribution	1315:1341	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	1	4	theme	human	156:160	arg1	health					162:167	human health	156:167	human health	156:167	A growing body of evidence has demonstrated the importance of the gut microbiome in human health.					
34714122	7	5	theme	freeze-thaw	1258:1268	arg1	conditions					1270:1279	different freeze-thaw conditions	1248:1279	different freeze-thaw conditions mimicking real-life situations in the process of distribution	1248:1341	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	9	6	theme	natural	1600:1606	arg1	cryoprotectants					1608:1622	natural cryoprotectants	1600:1622	natural cryoprotectants	1600:1622	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	3	7	theme	biological	503:512	arg1	activity					514:521	biological activity	503:521	biological activity	503:521	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	9	8	theme	human	1680:1684	arg1	specimens					1700:1708	invaluable human gut microbial specimens	1669:1708	invaluable human gut microbial specimens	1669:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	4	9	theme	cryopreservation	569:584	arg1	efficiency					586:595	the relative cryopreservation efficiency	556:595	the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples	556:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	5	10	theme	obligate	727:734	arg1	bacteria					746:753	two obligate anaerobic bacteria	723:753	two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity	723:837	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	5	11	theme	facultative	966:976	arg1	bacteria					1001:1008	mixed facultative and obligate anaerobic bacteria	960:1008	mixed facultative and obligate anaerobic bacteria	960:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	9	12	theme	long-term	1635:1643	arg1	viability					1656:1664	the long-term and stable viability	1631:1664	the long-term and stable viability of invaluable human gut microbial specimens	1631:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	4	13	theme	relative	560:567	arg1	efficiency					586:595	the relative cryopreservation efficiency	556:595	the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples	556:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	3	14	theme	optimized	309:317	arg1	method					336:341	an optimized cryopreservation method	306:341	an optimized cryopreservation method	306:341	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	5	15	theme	concentration-dependent	843:865	arg1	bacteria					867:874	concentration-dependent bacteria	843:874	concentration-dependent bacteria	843:874	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	4	16	theme	natural	619:625	arg1	cryoprotectants					627:641	different nontoxic natural cryoprotectants	600:641	different nontoxic natural cryoprotectants using infant fecal and meconium samples	600:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	1	17	theme	growing	74:80	arg1	body					82:85	A growing body	72:85	A growing body of evidence	72:97	A growing body of evidence has demonstrated the importance of the gut microbiome in human health.					
34714122	6	18	theme	rRNA	1136:1139	arg1	analyses					1152:1159	16S rRNA sequencing analyses	1132:1159	16S rRNA sequencing analyses	1132:1159	Finally, we explored the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation using 16S rRNA sequencing analyses.					
34714122	3	19	theme	cryopreservation	319:334	arg1	method					336:341	an optimized cryopreservation method	306:341	an optimized cryopreservation method	306:341	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	5	20	theme	bacterial	937:945	arg1	diversity					947:955	the viabilities and bacterial diversity	917:955	the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria	917:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	7	21	used	used	1243:1246	arg2	we					1240:1241	we	1240:1241	we	1240:1241	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	2	22	theme	diseases	251:258	arg1	mechanisms					228:237	the mechanisms	224:237	the mechanisms of relevant diseases	224:258	In general, fecal microbial samples are used to study the mechanisms of relevant diseases.					
34714122	6	23	theme	sequencing	1141:1150	arg1	analyses					1152:1159	16S rRNA sequencing analyses	1132:1159	16S rRNA sequencing analyses	1132:1159	Finally, we explored the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation using 16S rRNA sequencing analyses.					
34714122	3	24	theme	scientific	419:428	arg1	investigations					430:443	scientific investigations	419:443	scientific investigations	419:443	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	7	25	theme	real-life	1291:1299	arg1	situations					1301:1310	real-life situations	1291:1310	real-life situations in the process of distribution	1291:1341	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	9	26	theme	invaluable	1669:1678	arg1	specimens					1700:1708	invaluable human gut microbial specimens	1669:1708	invaluable human gut microbial specimens	1669:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	1	27	theme	evidence	90:97	arg1	body					82:85	A growing body	72:85	A growing body of evidence	72:97	A growing body of evidence has demonstrated the importance of the gut microbiome in human health.					
34714122	0	28	theme	Natural	47:53	arg1	Cryoprotectants					55:69	Natural Cryoprotectants	47:69	Natural Cryoprotectants	47:69	Cryopreservation of Infant Gut Microbiota with Natural Cryoprotectants.					
34714122	4	29	theme	meconium	666:673	arg1	samples					675:681	infant fecal and meconium samples	649:681	samples	675:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	0	30	theme	Gut	27:29	arg1	Microbiota					31:40	Infant Gut Microbiota	20:40	Infant Gut Microbiota	20:40	Cryopreservation of Infant Gut Microbiota with Natural Cryoprotectants.					
34714122	1	31	from	importance	120:129	arg1	health					162:167	human health	156:167	human health	156:167	A growing body of evidence has demonstrated the importance of the gut microbiome in human health.					
34714122	5	32	theme	anaerobic	991:999	arg1	bacteria					1001:1008	mixed facultative and obligate anaerobic bacteria	960:1008	mixed facultative and obligate anaerobic bacteria	960:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	2	33	used	used	210:213	arg2	samples					198:204	fecal microbial samples	182:204	fecal microbial samples	182:204	In general, fecal microbial samples are used to study the mechanisms of relevant diseases.					
34714122	4	34	theme	cryoprotectants	627:641	arg1	efficiency					586:595	the relative cryopreservation efficiency	556:595	the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples	556:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	0	35	theme	Infant	20:25	arg1	Microbiota					31:40	Infant Gut Microbiota	20:40	Infant Gut Microbiota	20:40	Cryopreservation of Infant Gut Microbiota with Natural Cryoprotectants.					
34714122	5	36	theme	obligate	982:989	arg1	bacteria					1001:1008	mixed facultative and obligate anaerobic bacteria	960:1008	mixed facultative and obligate anaerobic bacteria	960:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	5	37	theme	bacteria	1001:1008	arg1	diversity					947:955	the viabilities and bacterial diversity	917:955	the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria	917:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	3	38	theme	gut	452:454	arg1	microbiome					456:465	the gut microbiome	448:465	the gut microbiome	448:465	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	5	39	theme	microbial	779:787	arg1	strain					789:794	the experimental gut microbial strain	758:794	the experimental gut microbial strain	758:794	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	8	40	theme	fecal	1391:1395	arg1	sample					1397:1402	the infant fecal sample's	1380:1404	the infant fecal sample's cryopreservation	1380:1421	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	5	41	theme	facultative	707:717	arg1	viabilities					876:886	two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities	703:886	two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities	703:886	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	6	42	theme	infant	1090:1095	arg1	feces					1097:1101	infant feces	1090:1101	infant feces	1090:1101	Finally, we explored the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation using 16S rRNA sequencing analyses.					
34714122	9	43	theme	gut	1686:1688	arg1	specimens					1700:1708	invaluable human gut microbial specimens	1669:1708	invaluable human gut microbial specimens	1669:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	4	44	theme	infant	649:654	arg1	fecal					656:660	infant fecal and meconium samples	649:681	fecal	656:660	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	0	45	theme	Microbiota	31:40	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of Infant Gut Microbiota with Natural Cryoprotectants.	0:70	Cryopreservation of Infant Gut Microbiota with Natural Cryoprotectants.					
34714122	6	46	theme	cryoprotectants	1057:1071	arg1	effects					1036:1042	the effects	1032:1042	the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation	1032:1124	Finally, we explored the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation using 16S rRNA sequencing analyses.					
34714122	7	47	theme	different	1248:1256	arg1	conditions					1270:1279	different freeze-thaw conditions	1248:1279	different freeze-thaw conditions mimicking real-life situations in the process of distribution	1248:1341	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	9	48	theme	microbial	1690:1698	arg1	specimens					1700:1708	invaluable human gut microbial specimens	1669:1708	invaluable human gut microbial specimens	1669:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	8	49	theme	optimum	1492:1498	arg1	trehalose/betaine					1466:1482	200 mg/mL trehalose/betaine	1456:1482	200 mg/mL trehalose/betaine	1456:1482	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	8	49	theme	optimum	1492:1498	arg1	choice					1500:1505	the optimum choice	1488:1505	the optimum choice for meconium cryopreservation	1488:1535	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	8	50	theme	100 mg/mL	1427:1435	arg1	choice					1369:1374	the better choice	1358:1374	the better choice for the infant fecal sample's cryopreservation	1358:1421	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	8	50	theme	100 mg/mL	1427:1435	arg1	trehalose					1437:1445	100 mg/mL trehalose	1427:1445	100 mg/mL trehalose	1427:1445	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	8	51	theme	better	1362:1367	arg1	choice					1369:1374	the better choice	1358:1374	the better choice for the infant fecal sample's cryopreservation	1358:1421	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	8	51	theme	better	1362:1367	arg1	trehalose					1437:1445	100 mg/mL trehalose	1427:1445	100 mg/mL trehalose	1427:1445	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	3	52	theme	clinical	386:393	arg1	diagnosis					395:403	clinical diagnosis	386:403	clinical diagnosis	386:403	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	8	53	theme	meconium	1511:1518	arg1	cryopreservation					1520:1535	meconium cryopreservation	1511:1535	meconium cryopreservation	1511:1535	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	7	54	theme	cryoprotectants	1223:1237	arg1	effectiveness					1200:1212	the effectiveness	1196:1212	the effectiveness of these cryoprotectants	1196:1237	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	7	55	theme	distribution	1330:1341	arg1	process					1319:1325	the process	1315:1325	the process of distribution	1315:1341	In addition, to better understand the effectiveness of these cryoprotectants, we used different freeze-thaw conditions mimicking real-life situations in the process of distribution.					
34714122	9	56	theme	specimens	1700:1708	arg1	viability					1656:1664	the long-term and stable viability	1631:1664	the long-term and stable viability of invaluable human gut microbial specimens	1631:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	2	57	theme	microbial	188:196	arg1	samples					198:204	fecal microbial samples	182:204	fecal microbial samples	182:204	In general, fecal microbial samples are used to study the mechanisms of relevant diseases.					
34714122	2	58	theme	relevant	242:249	arg1	diseases					251:258	relevant diseases	242:258	relevant diseases	242:258	In general, fecal microbial samples are used to study the mechanisms of relevant diseases.					
34714122	0	59	with	Cryopreservation	0:15	arg1	Cryoprotectants					55:69	Natural Cryoprotectants	47:69	Natural Cryoprotectants	47:69	Cryopreservation of Infant Gut Microbiota with Natural Cryoprotectants.					
34714122	3	60	theme	microbiome	456:465	arg1	therapy					406:412	therapy	406:412	therapy	406:412	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	3	60	theme	microbiome	456:465	arg1	diagnosis					395:403	clinical diagnosis	386:403	clinical diagnosis	386:403	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	3	60	theme	microbiome	456:465	arg1	investigations					430:443	scientific investigations	419:443	scientific investigations	419:443	In this context, it is worth mentioning that an optimized cryopreservation method is urgently needed to successfully perform clinical diagnosis, therapy, and scientific investigations of the gut microbiome without affecting its viability and biological activity.					
34714122	8	61	theme	200 mg/mL	1456:1464	arg1	trehalose/betaine					1466:1482	200 mg/mL trehalose/betaine	1456:1482	200 mg/mL trehalose/betaine	1456:1482	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	8	61	theme	200 mg/mL	1456:1464	arg1	choice					1500:1505	the optimum choice	1488:1505	the optimum choice for meconium cryopreservation	1488:1535	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	5	62	theme	anaerobic	736:744	arg1	bacteria					746:753	two obligate anaerobic bacteria	723:753	two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity	723:837	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	9	63	theme	stable	1649:1654	arg1	viability					1656:1664	the long-term and stable viability	1631:1664	the long-term and stable viability of invaluable human gut microbial specimens	1631:1708	We hope that our results will shed light on the importance of natural cryoprotectants toward the long-term and stable viability of invaluable human gut microbial specimens.					
34714122	2	64	theme	fecal	182:186	arg1	samples					198:204	fecal microbial samples	182:204	fecal microbial samples	182:204	In general, fecal microbial samples are used to study the mechanisms of relevant diseases.					
34714122	8	65	theme	infant	1384:1389	arg1	sample					1397:1402	the infant fecal sample's	1380:1404	the infant fecal sample's cryopreservation	1380:1421	We found that the better choice for the infant fecal sample's cryopreservation was 100 mg/mL trehalose, whereas 200 mg/mL trehalose/betaine was the optimum choice for meconium cryopreservation.					
34714122	5	66	theme	viabilities	921:931	arg1	diversity					947:955	the viabilities and bacterial diversity	917:955	the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria	917:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	5	67	theme	mixed	960:964	arg1	bacteria					1001:1008	mixed facultative and obligate anaerobic bacteria	960:1008	mixed facultative and obligate anaerobic bacteria	960:1008	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	5	68	dep	facultative	707:717	arg1	bacteria					867:874	concentration-dependent bacteria	843:874	concentration-dependent bacteria	843:874	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	5	68	dep	facultative	707:717	arg1	bacteria					746:753	two obligate anaerobic bacteria	723:753	two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity	723:837	First, we selected two facultative and two obligate anaerobic bacteria as the experimental gut microbial strain to compare these cryoprotectants' toxicity and concentration-dependent bacteria viabilities after cryopreservation, then the viabilities and bacterial diversity of mixed facultative and obligate anaerobic bacteria.					
34714122	1	69	theme	gut	138:140	arg1	microbiome					142:151	the gut microbiome	134:151	the gut microbiome	134:151	A growing body of evidence has demonstrated the importance of the gut microbiome in human health.					
34714122	6	70	theme	16S	1132:1134	arg1	analyses					1152:1159	16S rRNA sequencing analyses	1132:1159	16S rRNA sequencing analyses	1132:1159	Finally, we explored the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation using 16S rRNA sequencing analyses.					
34714122	4	71	theme	nontoxic	610:617	arg1	cryoprotectants					627:641	different nontoxic natural cryoprotectants	600:641	different nontoxic natural cryoprotectants using infant fecal and meconium samples	600:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
34714122	6	72	theme	optimized	1047:1055	arg1	cryoprotectants					1057:1071	optimized cryoprotectants	1047:1071	optimized cryoprotectants for meconium and infant feces	1047:1101	Finally, we explored the effects of optimized cryoprotectants for meconium and infant feces after cryopreservation using 16S rRNA sequencing analyses.					
34714122	1	73	theme	microbiome	142:151	arg1	importance					120:129	the importance	116:129	the importance of the gut microbiome in human health	116:167	A growing body of evidence has demonstrated the importance of the gut microbiome in human health.					
34714122	4	74	theme	different	600:608	arg1	cryoprotectants					627:641	different nontoxic natural cryoprotectants	600:641	different nontoxic natural cryoprotectants using infant fecal and meconium samples	600:681	In this study, we aimed to test the relative cryopreservation efficiency of different nontoxic natural cryoprotectants using infant fecal and meconium samples.					
32507195	7	0	theme	platform	1179:1186	arg1	preparation					1142:1152	The successful preparation	1127:1152	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug	1127:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	6	1	theme	first-order	1026:1036	arg1	kinetics					1046:1053	first-order release kinetics	1026:1053	first-order release kinetics	1026:1053	Spin-films were more physically stable over 12 w; however, controlled release of ibuprofen was achieved mainly from hot-melt-extruded-films for 5 h. Both films have shown first-order release kinetics; whereby polymeric swelling and relaxation likely governed the release.					
32507195	2	2	theme	solid	448:452	arg1	dispersion					454:463	its amorphous solid dispersion	434:463	its amorphous solid dispersion	434:463	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	7	3	theme	cellaburate-colophony	1157:1177	arg1	platform					1179:1186	cellaburate-colophony platform	1157:1186	cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug	1157:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	4	4	theme	RH/22 °C.	686:694	arg1	Characterization					696:711	12 w at 54 %RH/22 °C. Characterization	674:711	12 w at 54 %RH/22 °C. Characterization involved using PLM/DSC/MTDSC/ATRFTIR/TGA/SEM and PXRD	674:765	Physical stability was evaluated for 12 w at 54 %RH/22 °C. Characterization involved using PLM/DSC/MTDSC/ATRFTIR/TGA/SEM and PXRD.					
32507195	6	5	theme	controlled	914:923	arg1	release					925:931	controlled release	914:931	controlled release of ibuprofen	914:944	Spin-films were more physically stable over 12 w; however, controlled release of ibuprofen was achieved mainly from hot-melt-extruded-films for 5 h. Both films have shown first-order release kinetics; whereby polymeric swelling and relaxation likely governed the release.					
32507195	3	6	theme	spin-coating	512:523	arg1	technologies					496:507	two preparation technologies	480:507	two preparation technologies of spin-coating and hot-melt-extrusion	480:546	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	1	7	from	materials	197:205	arg1	applications					225:236	pharmaceutical applications	210:236	pharmaceutical applications	210:236	Recently, there has been a rapid growth of using bio-based materials in pharmaceutical applications, due to their low cost and availability.					
32507195	1	8	theme	low	252:254	arg1	cost					256:259	their low cost	246:259	their low cost	246:259	Recently, there has been a rapid growth of using bio-based materials in pharmaceutical applications, due to their low cost and availability.					
32507195	7	9	theme	drug	1255:1258	arg1	profiles					1222:1229	tunable release profiles	1206:1229	tunable release profiles of poorly water-soluble drug	1206:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	7	10	theme	successful	1131:1140	arg1	preparation					1142:1152	The successful preparation	1127:1152	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug	1127:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	3	11	theme	technologies	496:507	arg1	effect					470:475	The effect	466:475	The effect of two preparation technologies of spin-coating and hot-melt-extrusion	466:546	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	7	12	theme	tunable	1206:1212	arg1	profiles					1222:1229	tunable release profiles	1206:1229	tunable release profiles of poorly water-soluble drug	1206:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	7	13	theme	water-soluble	1241:1253	arg1	drug					1255:1258	poorly water-soluble drug	1234:1258	poorly water-soluble drug	1234:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	2	14	theme	ibuprofen	411:419	arg1	release					421:427	ibuprofen release	411:427	ibuprofen release from its amorphous solid dispersion	411:463	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	7	15	theme	further	1284:1290	arg1	development					1306:1316	further drug delivery development	1284:1316	further drug delivery development	1284:1316	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	1	16	theme	rapid	165:169	arg1	growth					171:176	a rapid growth	163:176	a rapid growth of using bio-based materials in pharmaceutical applications, due to their low cost and availability	163:276	Recently, there has been a rapid growth of using bio-based materials in pharmaceutical applications, due to their low cost and availability.					
32507195	5	17	theme	colophony	826:834	arg1	films					848:852	35:65 colophony:cellaburate films	820:852	35:65 colophony:cellaburate films	820:852	Ibuprofen was amorphously-dispersed at 30 %(w/w) in 35:65 colophony:cellaburate films.					
32507195	2	18	theme	colophony	351:359	arg1	composition					302:312	natural composition	294:312	natural composition of cellaburate (cellulose-ester) and colophony (pine-resin)	294:372	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	0	19	theme	dispersion	24:33	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen	0:82	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen controlled release processed by HME and spin coating.					
32507195	0	20	theme	spin	124:127	arg1	coating					129:135	spin coating	124:135	spin coating	124:135	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen controlled release processed by HME and spin coating.					
32507195	3	21	theme	hot-melt-extrusion	529:546	arg1	technologies					496:507	two preparation technologies	480:507	two preparation technologies of spin-coating and hot-melt-extrusion	480:546	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	3	22	dep	in	598:599	arg1	vitro					601:605	vitro	601:605	vitro	601:605	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	0	23	theme	amorphous	14:22	arg1	dispersion					24:33	amorphous dispersion	14:33	amorphous dispersion of a cellulose ester-colophony mix for ibuprofen	14:82	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen controlled release processed by HME and spin coating.					
32507195	4	24	dep	%	685:685	arg1	54					682:683	54	682:683	54	682:683	Physical stability was evaluated for 12 w at 54 %RH/22 °C. Characterization involved using PLM/DSC/MTDSC/ATRFTIR/TGA/SEM and PXRD.					
32507195	2	25	from	dispersion	454:463	arg1	release					421:427	ibuprofen release	411:427	ibuprofen release from its amorphous solid dispersion	411:463	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	2	26	theme	amorphous	438:446	arg1	dispersion					454:463	its amorphous solid dispersion	434:463	its amorphous solid dispersion	434:463	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	1	27	theme	bio-based	187:195	arg1	materials					197:205	bio-based materials	187:205	bio-based materials in pharmaceutical applications	187:236	Recently, there has been a rapid growth of using bio-based materials in pharmaceutical applications, due to their low cost and availability.					
32507195	7	28	theme	release	1214:1220	arg1	profiles					1222:1229	tunable release profiles	1206:1229	tunable release profiles of poorly water-soluble drug	1206:1258	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	6	29	theme	polymeric	1064:1072	arg1	swelling					1074:1081	polymeric swelling	1064:1081	polymeric swelling	1064:1081	Spin-films were more physically stable over 12 w; however, controlled release of ibuprofen was achieved mainly from hot-melt-extruded-films for 5 h. Both films have shown first-order release kinetics; whereby polymeric swelling and relaxation likely governed the release.					
32507195	0	30	theme	ester-colophony	50:64	arg1	mix					66:68	a cellulose ester-colophony mix	38:68	a cellulose ester-colophony mix for ibuprofen	38:82	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen controlled release processed by HME and spin coating.					
32507195	7	31	theme	delivery	1297:1304	arg1	development					1306:1316	further drug delivery development	1284:1316	further drug delivery development	1284:1316	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	2	32	used	used	378:381	arg2	composition					302:312	natural composition	294:312	natural composition of cellaburate (cellulose-ester) and colophony (pine-resin)	294:372	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	2	33	theme	cellaburate	317:327	arg1	composition					302:312	natural composition	294:312	natural composition of cellaburate (cellulose-ester) and colophony (pine-resin)	294:372	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	0	34	theme	cellulose	40:48	arg1	mix					66:68	a cellulose ester-colophony mix	38:68	a cellulose ester-colophony mix for ibuprofen	38:82	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen controlled release processed by HME and spin coating.					
32507195	3	35	theme	physicochemical	567:581	arg1	properties					583:592	the physicochemical properties	563:592	the physicochemical properties	563:592	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	5	36	from	%	810:810	arg1	films					848:852	35:65 colophony:cellaburate films	820:852	35:65 colophony:cellaburate films	820:852	Ibuprofen was amorphously-dispersed at 30 %(w/w) in 35:65 colophony:cellaburate films.					
32507195	3	37	theme	dissolution/release	607:625	arg1	behavior					627:634	in vitro dissolution/release behavior	598:634	in vitro dissolution/release behavior	598:634	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	4	38	dep	Characterization	696:711	arg1	%					685:685	%	685:685	%	685:685	Physical stability was evaluated for 12 w at 54 %RH/22 °C. Characterization involved using PLM/DSC/MTDSC/ATRFTIR/TGA/SEM and PXRD.					
32507195	2	39	theme	natural	294:300	arg1	composition					302:312	natural composition	294:312	natural composition of cellaburate (cellulose-ester) and colophony (pine-resin)	294:372	In this study, natural composition of cellaburate (cellulose-ester) and colophony (pine-resin) was used to prepare films to control ibuprofen release from its amorphous solid dispersion.					
32507195	1	40	theme	pharmaceutical	210:223	arg1	applications					225:236	pharmaceutical applications	210:236	pharmaceutical applications	210:236	Recently, there has been a rapid growth of using bio-based materials in pharmaceutical applications, due to their low cost and availability.					
32507195	7	41	theme	drug	1292:1295	arg1	development					1306:1316	further drug delivery development	1284:1316	further drug delivery development	1284:1316	The successful preparation of cellaburate-colophony platform that has achieved tunable release profiles of poorly water-soluble drug holds the potential for further drug delivery development.					
32507195	3	42	theme	in	598:599	arg1	behavior					627:634	in vitro dissolution/release behavior	598:634	in vitro dissolution/release behavior	598:634	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	4	43	theme	Physical	637:644	arg1	stability					646:654	Physical stability	637:654	Physical stability	637:654	Physical stability was evaluated for 12 w at 54 %RH/22 °C. Characterization involved using PLM/DSC/MTDSC/ATRFTIR/TGA/SEM and PXRD.					
32507195	0	44	theme	mix	66:68	arg1	dispersion					24:33	amorphous dispersion	14:33	amorphous dispersion of a cellulose ester-colophony mix for ibuprofen	14:82	Evaluation of amorphous dispersion of a cellulose ester-colophony mix for ibuprofen controlled release processed by HME and spin coating.					
32507195	6	45	theme	ibuprofen	936:944	arg1	release					925:931	controlled release	914:931	controlled release of ibuprofen	914:944	Spin-films were more physically stable over 12 w; however, controlled release of ibuprofen was achieved mainly from hot-melt-extruded-films for 5 h. Both films have shown first-order release kinetics; whereby polymeric swelling and relaxation likely governed the release.					
32507195	5	46	theme	cellaburate	836:846	arg1	films					848:852	35:65 colophony:cellaburate films	820:852	35:65 colophony:cellaburate films	820:852	Ibuprofen was amorphously-dispersed at 30 %(w/w) in 35:65 colophony:cellaburate films.					
32507195	3	47	theme	preparation	484:494	arg1	technologies					496:507	two preparation technologies	480:507	two preparation technologies of spin-coating and hot-melt-extrusion	480:546	The effect of two preparation technologies of spin-coating and hot-melt-extrusion was studied on the physicochemical properties and in vitro dissolution/release behavior.					
32507195	6	48	theme	release	1038:1044	arg1	kinetics					1046:1053	first-order release kinetics	1026:1053	first-order release kinetics	1026:1053	Spin-films were more physically stable over 12 w; however, controlled release of ibuprofen was achieved mainly from hot-melt-extruded-films for 5 h. Both films have shown first-order release kinetics; whereby polymeric swelling and relaxation likely governed the release.					
34246963	3	0	theme	magnetic	642:649	arg1	merits					630:635	many merits	625:635	many merits	625:635	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	3	0	theme	magnetic	642:649	arg1	sorbent					651:657	a magnetic sorbent	640:657	a magnetic sorbent	640:657	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	4	1	theme	method	813:818	arg1	linearity					785:793	The favourable linearity	770:793	The favourable linearity of this MSPE-HPLC method	770:818	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	1	2	theme	Chitosan-modified	168:184	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	2	theme	Chitosan-modified	168:184	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	0	3	theme	effective	88:96	arg1	enrichment					98:107	effective enrichment	88:107	effective enrichment	88:107	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	1	4	theme	4-methyl	331:338	arg1	4-MHA					355:359	4-MHA	355:359	4-MHA	355:359	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	4	theme	4-methyl	331:338	arg1	acid					349:352	4-methyl hippuric acid	331:352	4-methyl hippuric acid (4-MHA)	331:360	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	4	5	theme	MSPE-HPLC	803:811	arg1	method					813:818	this MSPE-HPLC method	798:818	this MSPE-HPLC method	798:818	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	1	6	theme	magnetic	186:193	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	6	theme	magnetic	186:193	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	4	7	theme	μg	847:848	arg1	L-1					850:852	1-1000 μg L-1	840:852	1-1000 μg L-1	840:852	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	1	8	theme	hippuric	340:347	arg1	4-MHA					355:359	4-MHA	355:359	4-MHA	355:359	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	8	theme	hippuric	340:347	arg1	acid					349:352	4-methyl hippuric acid	331:352	4-methyl hippuric acid (4-MHA)	331:360	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	2	9	theme	low	555:557	arg1	density					559:565	low density	555:565	low density	555:565	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	1	10	theme	acid	349:352	arg1	detection					295:303	detection	295:303	detection	295:303	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	10	theme	acid	349:352	arg1	enrichment					280:289	enrichment	280:289	enrichment	280:289	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	6	11	theme	great	1099:1103	arg1	potential					1105:1113	great potential	1099:1113	great potential	1099:1113	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	6	11	theme	great	1099:1103	arg1	candidate					1120:1128	a candidate	1118:1128	a candidate for preconcentration	1118:1149	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	0	12	theme	hippuric	126:133	arg1	acid					135:138	hippuric acid	126:138	hippuric acid	126:138	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	1	13	theme	Schiff	195:200	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	13	theme	Schiff	195:200	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	2	14	theme	framework	466:474	arg1	SNW					430:432	The SNW	426:432	The SNW	426:432	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	2	14	theme	framework	466:474	arg1	framework					466:474	the covalent organic framework	445:474	the covalent organic framework	445:474	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	2	14	theme	framework	466:474	arg1	one					438:440	one	438:440	one	438:440	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	4	15	theme	favourable	774:783	arg1	linearity					785:793	The favourable linearity	770:793	The favourable linearity of this MSPE-HPLC method	770:818	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	5	16	from	%	988:988	arg1	range					963:967	range	963:967	range	963:967	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	5	16	from	%	988:988	arg1	recoveries					930:939	The recoveries	926:939	The recoveries in urine samples	926:956	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	1	17	theme	base	202:205	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	17	theme	base	202:205	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	3	18	theme	SNW	608:610	arg1	Chitosan					612:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	5	19	from	recoveries	930:939	arg1	samples					950:956	urine samples	944:956	urine samples	944:956	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	6	20	theme	@	1079:1079	arg1	Chitosan					1080:1087	Fe3O4@SNW@Chitosan	1070:1087	Fe3O4@SNW@Chitosan	1070:1087	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	2	21	theme	solvent	536:542	arg1	stability					544:552	solvent stability	536:552	solvent stability	536:552	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	1	22	theme	network	207:213	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	22	theme	network	207:213	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	23	dep	enrichment	280:289	arg1	the					276:278	the	276:278	the	276:278	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	2	24	theme	accessible	571:580	arg1	pores					582:586	accessible pores	571:586	accessible pores	571:586	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	3	25	theme	unique	712:717	arg1	structure					735:743	unique ordered channel structure	712:743	unique ordered channel structure	712:743	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	1	26	theme	composite	215:223	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	26	theme	composite	215:223	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	0	27	theme	magnetic	33:40	arg1	composite					62:70	chitosan-modified magnetic Schiff base network composite	15:70	chitosan-modified magnetic Schiff base network composite	15:70	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	3	28	theme	uniform	693:699	arg1	size					706:709	uniform pore size	693:709	uniform pore size	693:709	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	4	29	theme	L-1	850:852	arg1	range					831:835	the range	827:835	the range of 1-1000 μg L-1	827:852	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	2	30	dep	constructed	483:493	arg1	shown					519:523	shown	519:523	shown comprising solvent stability, low density and accessible pores	519:586	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	0	31	theme	chitosan-modified	15:31	arg1	composite					62:70	chitosan-modified magnetic Schiff base network composite	15:70	chitosan-modified magnetic Schiff base network composite	15:70	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	3	32	theme	hydrophilic	672:682	arg1	surface					684:690	a hydrophilic surface	670:690	a hydrophilic surface	670:690	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	0	33	theme	acid	135:138	arg1	detection					113:121	detection	113:121	detection	113:121	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	0	33	theme	acid	135:138	arg1	enrichment					98:107	effective enrichment	88:107	effective enrichment	88:107	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	3	34	theme	@	607:607	arg1	Chitosan					612:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	5	35	with	range	963:967	arg1	RSD					999:1001	the RSD	995:1001	the RSD less than 9.6 %	995:1017	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	0	36	theme	base	49:52	arg1	composite					62:70	chitosan-modified magnetic Schiff base network composite	15:70	chitosan-modified magnetic Schiff base network composite	15:70	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	0	37	theme	hippuric	153:160	arg1	acid					162:165	4-methyl hippuric acid	144:165	4-methyl hippuric acid	144:165	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	1	38	theme	magnetic	366:373	arg1	MSPE					399:402	MSPE	399:402	MSPE	399:402	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	38	theme	magnetic	366:373	arg1	extraction					387:396	magnetic solid phase extraction	366:396	magnetic solid phase extraction (MSPE) connected with HPLC	366:423	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	6	39	theme	@	1075:1075	arg1	Chitosan					1080:1087	Fe3O4@SNW@Chitosan	1070:1087	Fe3O4@SNW@Chitosan	1070:1087	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	3	40	contain	has	621:623	arg1	Chitosan					612:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	3	40	contain	has	621:623	arg2	sorbent					651:657	a magnetic sorbent	640:657	a magnetic sorbent	640:657	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	3	40	contain	has	621:623	arg2	merits					630:635	many merits	625:635	many merits	625:635	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	1	41	theme	Fe3O4	238:242	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	41	theme	Fe3O4	238:242	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	0	42	theme	Schiff	42:47	arg1	composite					62:70	chitosan-modified magnetic Schiff base network composite	15:70	chitosan-modified magnetic Schiff base network composite	15:70	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	1	43	theme	hippuric	308:315	arg1	acid					317:320	hippuric acid	308:320	hippuric acid (HA)	308:325	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	43	theme	hippuric	308:315	arg1	HA					323:324	HA	323:324	HA	323:324	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	0	44	theme	4-methyl	144:151	arg1	acid					162:165	4-methyl hippuric acid	144:165	4-methyl hippuric acid	144:165	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	1	45	theme	solid	375:379	arg1	MSPE					399:402	MSPE	399:402	MSPE	399:402	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	45	theme	solid	375:379	arg1	extraction					387:396	magnetic solid phase extraction	366:396	magnetic solid phase extraction (MSPE) connected with HPLC	366:423	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	6	46	theme	Fe3O4	1070:1074	arg1	Chitosan					1080:1087	Fe3O4@SNW@Chitosan	1070:1087	Fe3O4@SNW@Chitosan	1070:1087	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	1	47	theme	@	243:243	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	47	theme	@	243:243	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	0	48	theme	composite	62:70	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan-modified magnetic Schiff base network composite	0:70	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	1	49	theme	acid	317:320	arg1	detection					295:303	detection	295:303	detection	295:303	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	49	theme	acid	317:320	arg1	enrichment					280:289	enrichment	280:289	enrichment	280:289	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	50	theme	phase	381:385	arg1	MSPE					399:402	MSPE	399:402	MSPE	399:402	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	50	theme	phase	381:385	arg1	extraction					387:396	magnetic solid phase extraction	366:396	magnetic solid phase extraction (MSPE) connected with HPLC	366:423	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	5	51	dep	109.0	982:986	arg1	to					979:980	to	979:980	to	979:980	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	6	52	theme	SNW	1076:1078	arg1	Chitosan					1080:1087	Fe3O4@SNW@Chitosan	1070:1087	Fe3O4@SNW@Chitosan	1070:1087	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	0	53	theme	network	54:60	arg1	composite					62:70	chitosan-modified magnetic Schiff base network composite	15:70	chitosan-modified magnetic Schiff base network composite	15:70	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	3	54	theme	pore	701:704	arg1	size					706:709	uniform pore size	693:709	uniform pore size	693:709	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	0	55	theme	acid	162:165	arg1	detection					113:121	detection	113:121	detection	113:121	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	0	55	theme	acid	162:165	arg1	enrichment					98:107	effective enrichment	88:107	effective enrichment	88:107	Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid.					
34246963	3	56	theme	Fe3O4	602:606	arg1	Chitosan					612:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	4	57	theme	4-MHA	874:878	arg1	LODs					859:862	LODs	859:862	LODs of HA and 4-MHA	859:878	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	3	58	theme	obtained	593:600	arg1	Chitosan					612:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	6	59	theme	4-MHA	1063:1067	arg1	enrichment					1042:1051	the enrichment	1038:1051	the enrichment of HA and 4-MHA	1038:1067	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	3	60	theme	many	625:628	arg1	merits					630:635	many merits	625:635	many merits	625:635	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	3	60	theme	many	625:628	arg1	sorbent					651:657	a magnetic sorbent	640:657	a magnetic sorbent	640:657	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	1	61	theme	SNW	244:246	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	61	theme	SNW	244:246	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	3	62	theme	@	611:611	arg1	Chitosan					612:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan	589:619	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	6	63	theme	HA	1056:1057	arg1	enrichment					1042:1051	the enrichment	1038:1051	the enrichment of HA and 4-MHA	1038:1067	When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration.					
34246963	1	64	theme	@	247:247	arg1	Chitosan					248:255	Fe3O4@SNW@Chitosan	238:255	Fe3O4@SNW@Chitosan	238:255	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	1	64	theme	@	247:247	arg1	nanospheres					225:235	Chitosan-modified magnetic Schiff base network composite nanospheres	168:235	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan)	168:256	Chitosan-modified magnetic Schiff base network composite nanospheres (Fe3O4@SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC.					
34246963	2	65	theme	organic	458:464	arg1	framework					466:474	the covalent organic framework	445:474	the covalent organic framework	445:474	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	5	66	theme	urine	944:948	arg1	samples					950:956	urine samples	944:956	urine samples	944:956	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	5	67	theme	less	1003:1006	arg1	RSD					999:1001	the RSD	995:1001	the RSD less than 9.6 %	995:1017	The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %.					
34246963	3	68	theme	ordered	719:725	arg1	structure					735:743	unique ordered channel structure	712:743	unique ordered channel structure	712:743	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	2	69	theme	covalent	449:456	arg1	framework					466:474	the covalent organic framework	445:474	the covalent organic framework	445:474	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
34246963	3	70	theme	channel	727:733	arg1	structure					735:743	unique ordered channel structure	712:743	unique ordered channel structure	712:743	The obtained Fe3O4@SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism.					
34246963	4	71	theme	HA	867:868	arg1	LODs					859:862	LODs	859:862	LODs of HA and 4-MHA	859:878	The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 μg L-1, and LODs of HA and 4-MHA were 0.3 μg L-1 and 0.2 μg L-1, respectively.					
34246963	2	72	theme	covalent	503:510	arg1	bonds					512:516	covalent bonds	503:516	covalent bonds	503:516	The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores.					
32464208	5	0	theme	IR	997:998	arg1	analysis					1008:1015	the IR and XPS analysis	993:1015	analysis	1008:1015	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	8	1	theme	high	1318:1321	arg1	capacity					1332:1339	high sorption capacity	1318:1339	high sorption capacity	1318:1339	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	1	2	theme	porous	147:152	arg1	nanotubes					183:191	The porous chitosan/carboxylated carbon nanotubes	143:191	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents	143:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	5	3	dep	U	955:955	arg1	sorption					961:968	sorption	961:968	sorption	961:968	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	3	dep	U	955:955	arg1	VI					957:958	VI	957:958	VI	957:958	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	0	4	theme	efficient	87:95	arg1	removal					97:103	the efficient removal	83:103	the efficient removal of uranium(VI) from aqueous solution	83:140	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	4	5	theme	pH 5.0	782:787	arg1	K					797:797	pH 5.0 and 298 K	782:797	K	797:797	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	5	6	from	chemisorption	804:816	arg1	groups					927:932	carboxylic and amine groups	906:932	groups	927:932	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	6	from	chemisorption	804:816	arg1	sites					899:903	the active sites	888:903	the active sites (carboxylic and amine groups)	888:933	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	1	7	theme	chitosan/carboxylated	154:174	arg1	nanotubes					183:191	The porous chitosan/carboxylated carbon nanotubes	143:191	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents	143:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	0	8	theme	uranium	108:114	arg1	removal					97:103	the efficient removal	83:103	the efficient removal of uranium(VI) from aqueous solution	83:140	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	5	9	theme	XPS	1004:1006	arg1	analysis					1008:1015	the IR and XPS analysis	993:1015	analysis	1008:1015	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	6	10	theme	chemisorption	1157:1169	arg1	mechanism					1171:1179	chemisorption mechanism	1157:1179	chemisorption mechanism	1157:1179	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	8	11	theme	unique	1287:1292	arg1	recovery					1365:1372	easy recovery	1360:1372	easy recovery	1360:1372	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	8	11	theme	unique	1287:1292	arg1	kinetic					1347:1353	kinetic	1347:1353	kinetic	1347:1353	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	8	11	theme	unique	1287:1292	arg1	capacity					1332:1339	high sorption capacity	1318:1339	high sorption capacity	1318:1339	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	8	11	theme	unique	1287:1292	arg1	characteristics					1294:1308	The unique characteristics	1283:1308	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution	1283:1386	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	3	12	theme	aerogels	617:624	arg1	structure					575:583	the porous structure	564:583	the porous structure	564:583	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	3	12	theme	aerogels	617:624	arg1	ability					602:608	the sorption ability	589:608	the sorption ability of the aerogels	589:624	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	6	13	theme	isotherms	1114:1122	arg1	Meanwhile					1018:1026	Meanwhile	1018:1026	Meanwhile	1018:1026	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	6	13	theme	isotherms	1114:1122	arg1	good-fitting					1033:1044	the good-fitting	1029:1044	the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model	1029:1140	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	5	14	dep	chemisorption	804:816	arg1	The					800:802	The	800:802	The	800:802	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	4	15	theme	298	793:795	arg1	K					797:797	pH 5.0 and 298 K	782:797	K	797:797	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	3	16	theme	aerogels	499:506	arg1	capacity					483:490	The sorption capacity	470:490	The sorption capacity of the aerogels	470:506	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	1	17	theme	efficient	271:279	arg1	removal					281:287	the efficient removal	267:287	the efficient removal of U(VI) from aqueous solution	267:318	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	5	18	theme	surface	821:827	arg1	complexation					829:840	surface complexation	821:840	surface complexation	821:840	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	7	19	dep	sorption	1224:1231	arg1	VI					1220:1221	VI	1220:1221	VI	1220:1221	The thermodynamic data suggest that U(VI) sorption on CS-CCN aerogel is endothermic and spontaneous.					
32464208	0	20	dep	uranium	108:114	arg1	VI					116:117	VI	116:117	VI	116:117	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	7	21	from	sorption	1224:1231	arg1	aerogel					1243:1249	CS-CCN aerogel	1236:1249	CS-CCN aerogel	1236:1249	The thermodynamic data suggest that U(VI) sorption on CS-CCN aerogel is endothermic and spontaneous.					
32464208	1	22	theme	carbon	176:181	arg1	nanotubes					183:191	The porous chitosan/carboxylated carbon nanotubes	143:191	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents	143:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	3	23	contain	has	538:540	arg2	impact					554:559	significant impact	542:559	significant impact	542:559	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	3	23	contain	has	538:540	arg1	content					523:529	CCN content	519:529	CCN content	519:529	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	2	24	dep	characterizations	421:437	arg1	etc					463:465	etc	463:465	etc	463:465	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	2	24	dep	characterizations	421:437	arg1	XRD					458:460	XRD	458:460	XRD	458:460	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	2	24	dep	characterizations	421:437	arg1	TEM					453:455	TEM	453:455	TEM	453:455	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	2	24	dep	characterizations	421:437	arg1	SEM					448:450	SEM	448:450	SEM	448:450	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	5	25	theme	active	892:897	arg1	groups					927:932	carboxylic and amine groups	906:932	groups	927:932	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	25	theme	active	892:897	arg1	sites					899:903	the active sites	888:903	the active sites (carboxylic and amine groups)	888:933	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	2	26	theme	porous	377:382	arg1	structure					384:392	highly porous structure	370:392	highly porous structure	370:392	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	3	27	theme	sorption	474:481	arg1	capacity					483:490	The sorption capacity	470:490	The sorption capacity of the aerogels	470:506	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	6	28	theme	Langmuir	1127:1134	arg1	model					1136:1140	Langmuir model	1127:1140	Langmuir model	1127:1140	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	3	29	theme	CCN	519:521	arg1	content					523:529	CCN content	519:529	CCN content	519:529	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	8	30	theme	CS-CCN	1393:1398	arg1	aerogels					1400:1407	CS-CCN aerogels	1393:1407	CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater	1393:1478	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	1	31	theme	composite	193:201	arg1	CS-CCN					213:218	CS-CCN	213:218	CS-CCN	213:218	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	1	31	theme	composite	193:201	arg1	aerogels					203:210	composite aerogels	193:210	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents	143:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	6	32	theme	kinetics	1063:1070	arg1	isotherms					1114:1122	both sorption kinetics by pseudo-second-order model and sorption isotherms	1049:1122	both sorption kinetics by pseudo-second-order model and sorption isotherms	1049:1122	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	2	33	dep	such	440:443	arg1	as					445:446	as	445:446	as	445:446	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	5	34	theme	O/N	861:863	arg1	electrons					875:883	O/N lone pair electrons	861:883	O/N lone pair electrons	861:883	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	7	35	theme	CS-CCN	1236:1241	arg1	aerogel					1243:1249	CS-CCN aerogel	1236:1249	CS-CCN aerogel	1236:1249	The thermodynamic data suggest that U(VI) sorption on CS-CCN aerogel is endothermic and spontaneous.					
32464208	7	36	theme	U	1218:1218	arg1	sorption					1224:1231	U(VI) sorption	1218:1231	U(VI) sorption on CS-CCN aerogel	1218:1249	The thermodynamic data suggest that U(VI) sorption on CS-CCN aerogel is endothermic and spontaneous.					
32464208	0	37	theme	chitosan/carboxylated	22:42	arg1	carbon					44:49	porous chitosan/carboxylated carbon	15:49	porous chitosan/carboxylated carbon	15:49	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	5	38	theme	carboxylic	906:915	arg1	groups					927:932	carboxylic and amine groups	906:932	groups	927:932	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	38	theme	carboxylic	906:915	arg1	sites					899:903	the active sites	888:903	the active sites (carboxylic and amine groups)	888:933	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	39	from	complexation	829:840	arg1	groups					927:932	carboxylic and amine groups	906:932	groups	927:932	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	39	from	complexation	829:840	arg1	sites					899:903	the active sites	888:903	the active sites (carboxylic and amine groups)	888:933	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	4	40	theme	U	683:683	arg1	sorption					689:696	U(VI) sorption	683:696	U(VI) sorption	683:696	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	5	41	theme	pair	870:873	arg1	electrons					875:883	O/N lone pair electrons	861:883	O/N lone pair electrons	861:883	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	2	42	theme	different	411:419	arg1	characterizations					421:437	different characterizations	411:437	different characterizations (such as SEM, TEM, XRD, etc.)	411:467	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	0	43	theme	porous	15:20	arg1	carbon					44:49	porous chitosan/carboxylated carbon	15:49	porous chitosan/carboxylated carbon	15:49	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	1	44	theme	U	292:292	arg1	removal					281:287	the efficient removal	267:287	the efficient removal of U(VI) from aqueous solution	267:318	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	4	45	theme	CS-CCN	631:636	arg1	aerogels					638:645	The CS-CCN aerogels	627:645	The CS-CCN aerogels	627:645	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	4	45	theme	CS-CCN	631:636	arg1	effective					669:677	effective	669:677	effective	669:677	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	4	46	dep	sorption	689:696	arg1	VI					685:686	VI	685:686	VI	685:686	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	8	47	theme	radioactive	1457:1467	arg1	wastewater					1469:1478	radioactive wastewater	1457:1478	radioactive wastewater	1457:1478	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	3	48	theme	sorption	593:600	arg1	ability					602:608	the sorption ability	589:608	the sorption ability of the aerogels	589:624	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	5	49	theme	amine	921:925	arg1	groups					927:932	carboxylic and amine groups	906:932	groups	927:932	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	5	49	theme	amine	921:925	arg1	sites					899:903	the active sites	888:903	the active sites (carboxylic and amine groups)	888:933	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	6	50	theme	sorption	1054:1061	arg1	kinetics					1063:1070	sorption kinetics	1054:1070	sorption kinetics by pseudo-second-order model	1054:1099	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	0	51	theme	carbon	44:49	arg1	Preparation					0:10	Preparation	0:10	Preparation of porous chitosan/carboxylated carbon	0:49	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	0	52	theme	aqueous	125:131	arg1	solution					133:140	aqueous solution	125:140	aqueous solution	125:140	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	3	53	theme	significant	542:552	arg1	impact					554:559	significant impact	542:559	significant impact	542:559	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	7	54	theme	thermodynamic	1186:1198	arg1	data					1200:1203	The thermodynamic data	1182:1203	The thermodynamic data	1182:1203	The thermodynamic data suggest that U(VI) sorption on CS-CCN aerogel is endothermic and spontaneous.					
32464208	1	55	from	solution	311:318	arg1	removal					281:287	the efficient removal	267:287	the efficient removal of U(VI) from aqueous solution	267:318	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	8	56	theme	efficient	1417:1425	arg1	sorbents					1427:1434	very efficient sorbents	1412:1434	very efficient sorbents	1412:1434	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	4	57	theme	CS-CCN2	744:750	arg1	aerogel					752:758	CS-CCN2 aerogel	744:758	CS-CCN2 aerogel	744:758	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	8	58	from	solution	1379:1386	arg1	recovery					1365:1372	easy recovery	1360:1372	easy recovery	1360:1372	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	8	58	from	solution	1379:1386	arg1	capacity					1332:1339	high sorption capacity	1318:1339	high sorption capacity	1318:1339	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	2	59	theme	successful	325:334	arg1	formation					336:344	The successful formation	321:344	The successful formation of CS-CCN aerogels with highly porous structure	321:392	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	0	60	theme	composite	60:68	arg1	aerogels					70:77	composite aerogels	60:77	composite aerogels for the efficient removal of uranium(VI) from aqueous solution	60:140	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	6	61	theme	sorption	1105:1112	arg1	isotherms					1114:1122	both sorption kinetics by pseudo-second-order model and sorption isotherms	1049:1122	both sorption kinetics by pseudo-second-order model and sorption isotherms	1049:1122	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	1	62	with	aerogels	203:210	arg1	contents					240:247	different CCN contents	226:247	different CCN contents	226:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	1	63	dep	nanotubes	183:191	arg1	CS-CCN					213:218	CS-CCN	213:218	CS-CCN	213:218	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	1	63	dep	nanotubes	183:191	arg1	aerogels					203:210	composite aerogels	193:210	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents	143:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	8	64	theme	sorption	1323:1330	arg1	capacity					1332:1339	high sorption capacity	1318:1339	high sorption capacity	1318:1339	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	1	65	theme	aqueous	303:309	arg1	solution					311:318	aqueous solution	303:318	aqueous solution	303:318	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	2	66	with	formation	336:344	arg1	structure					384:392	highly porous structure	370:392	highly porous structure	370:392	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	6	67	theme	pseudo-second-order	1075:1093	arg1	model					1095:1099	pseudo-second-order model	1075:1099	pseudo-second-order model	1075:1099	Meanwhile, the good-fitting of both sorption kinetics by pseudo-second-order model and sorption isotherms by Langmuir model also indicates chemisorption mechanism.					
32464208	4	68	theme	sorption	722:729	arg1	capacity					731:738	the maximum mono-layer sorption capacity	699:738	the maximum mono-layer sorption capacity for CS-CCN2 aerogel	699:758	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	5	69	theme	lone	865:868	arg1	electrons					875:883	O/N lone pair electrons	861:883	O/N lone pair electrons	861:883	The chemisorption or surface complexation through sharing of O/N lone pair electrons on the active sites (carboxylic and amine groups) was responsible for U(VI) sorption, which is confirmed by the IR and XPS analysis.					
32464208	0	70	from	solution	133:140	arg1	removal					97:103	the efficient removal	83:103	the efficient removal of uranium(VI) from aqueous solution	83:140	Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.					
32464208	8	71	theme	easy	1360:1363	arg1	recovery					1365:1372	easy recovery	1360:1372	easy recovery	1360:1372	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	3	72	theme	porous	568:573	arg1	structure					575:583	the porous structure	564:583	the porous structure	564:583	The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels.					
32464208	2	73	theme	CS-CCN	349:354	arg1	aerogels					356:363	CS-CCN aerogels	349:363	CS-CCN aerogels	349:363	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	1	74	theme	CCN	236:238	arg1	contents					240:247	different CCN contents	226:247	different CCN contents	226:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	4	75	theme	mono-layer	711:720	arg1	capacity					731:738	the maximum mono-layer sorption capacity	699:738	the maximum mono-layer sorption capacity for CS-CCN2 aerogel	699:758	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	2	76	theme	aerogels	356:363	arg1	formation					336:344	The successful formation	321:344	The successful formation of CS-CCN aerogels with highly porous structure	321:392	The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.).					
32464208	8	77	dep	aerogels	1400:1407	arg1	sorbents					1427:1434	very efficient sorbents	1412:1434	very efficient sorbents	1412:1434	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
32464208	1	78	dep	U	292:292	arg1	VI					294:295	VI	294:295	VI	294:295	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	4	79	theme	maximum	703:709	arg1	capacity					731:738	the maximum mono-layer sorption capacity	699:738	the maximum mono-layer sorption capacity for CS-CCN2 aerogel	699:758	The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aerogel reached 307.5 mg/g at pH 5.0 and 298 K.					
32464208	1	80	theme	different	226:234	arg1	contents					240:247	different CCN contents	226:247	different CCN contents	226:247	The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution.					
32464208	8	81	theme	wastewater	1469:1478	arg1	treatment					1444:1452	the treatment	1440:1452	the treatment of radioactive wastewater	1440:1478	The unique characteristics such as high sorption capacity, fast kinetic, and easy recovery from solution make CS-CCN aerogels be very efficient sorbents for the treatment of radioactive wastewater.					
33917637	2	0	from	effects	499:505	arg1	properties					522:531	the sensory properties	510:531	the sensory properties of SEE	510:538	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	2	1	theme	protein-carbohydrate	580:599	arg1	interactions					601:612	protein-carbohydrate interactions	580:612	protein-carbohydrate interactions	580:612	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	3	2	theme	squid	765:769	arg1	mantle					771:776	squid mantle	765:776	squid mantle	765:776	The objective of this research was to study the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn.					
33917637	7	3	theme	protein-protein	1360:1374	arg1	interaction					1376:1386	the protein-protein interaction	1356:1386	the protein-protein interaction	1356:1386	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	2	4	theme	negative	490:497	arg1	effects					499:505	negative effects	490:505	negative effects on the sensory properties of SEE	490:538	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	5	5	theme	biological	1114:1123	arg1	value					1125:1129	biological value	1114:1129	biological value (>93%)	1114:1136	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	5	theme	biological	1114:1123	arg1	%					1135:1135	>93%	1132:1135	>93%	1132:1135	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	0	6	theme	Potato	72:77	arg1	Mixtures					60:67	a Mixtures	58:67	a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC	58:148	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	4	7	dep	infrared	884:891	arg1	FTIR					894:897	FTIR	894:897	FTIR	894:897	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	2	8	theme	SEE	536:538	arg1	properties					522:531	the sensory properties	510:531	the sensory properties of SEE	510:538	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	7	9	theme	responsible	1340:1350	arg1	groups					1324:1329	the amine groups I and II	1314:1338	the amine groups I and II responsible for the protein-protein interaction	1314:1386	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	9	theme	responsible	1340:1350	arg1	II					1337:1338	II	1337:1338	II	1337:1338	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	9	theme	responsible	1340:1350	arg1	I					1331:1331	I	1331:1331	I	1331:1331	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	5	10	theme	high	1080:1083	arg1	content					1093:1099	a high protein content	1078:1099	a high protein content (40-85%)	1078:1108	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	10	theme	high	1080:1083	arg1	%					1107:1107	40-85%	1102:1107	40-85%	1102:1107	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	6	11	theme	extruded	1219:1226	arg1	samples					1228:1234	extruded samples	1219:1234	extruded samples	1219:1234	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	1	12	theme	high	345:348	arg1	content					358:364	its high protein content	341:364	its high protein content	341:364	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	7	13	theme	extrusion	1288:1296	arg1	process					1298:1304	The extrusion process	1284:1304	The extrusion process	1284:1304	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	5	14	theme	protein	1085:1091	arg1	content					1093:1099	a high protein content	1078:1099	a high protein content (40-85%)	1078:1108	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	14	theme	protein	1085:1091	arg1	%					1107:1107	40-85%	1102:1107	40-85%	1102:1107	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	0	15	theme	Corn	83:86	arg1	Starch					88:93	Corn Starch	83:93	Corn Starch	83:93	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	6	16	theme	melting	1143:1149	arg1	Tm					1164:1165	Tm	1164:1165	Tm	1164:1165	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	6	16	theme	melting	1143:1149	arg1	temperature					1151:1161	The melting temperature	1139:1161	The melting temperature (Tm)	1139:1166	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	1	17	theme	protein	350:356	arg1	content					358:364	its high protein content	341:364	its high protein content	341:364	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	4	18	theme	complexes	995:1003	arg1	regions					1022:1028	the possible regions	1009:1028	the possible regions responsible for their interactions	1009:1063	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	4	18	theme	complexes	995:1003	arg1	formation					967:975	the formation	963:975	the formation of protein-starch complexes	963:1003	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	4	19	theme	protein	828:834	arg1	digestibility					836:848	protein digestibility	828:848	protein digestibility	828:848	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	2	20	theme	sensory	514:520	arg1	properties					522:531	the sensory properties	510:531	the sensory properties of SEE	510:538	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	3	21	dep	SEE	745:747	arg1	elaborated					749:758	elaborated	749:758	SEE elaborated with squid mantle, potato and corn	745:793	The objective of this research was to study the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn.					
33917637	4	22	used	used	949:952	arg2	Calorimetry					926:936	Differential Scanning Calorimetry	904:936	Differential Scanning Calorimetry (DSC)	904:942	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	4	22	used	used	949:952	arg2	DSC					939:941	DSC	939:941	DSC	939:941	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	4	23	theme	possible	1013:1020	arg1	regions					1022:1028	the possible regions	1009:1028	the possible regions responsible for their interactions	1009:1063	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	6	24	theme	squid	1269:1273	arg1	content					1275:1281	the squid content	1265:1281	the squid content	1265:1281	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	2	25	theme	effect	624:629	arg1	type					572:575	the type	568:575	the type of protein-carbohydrate interactions and their effect on these properties	568:649	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	2	26	theme	proteins	428:435	arg1	interaction					413:423	the interaction	409:423	the interaction of proteins of animal origin with starch during extrusion	409:481	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	4	27	theme	nutritional	800:810	arg1	composition					812:822	The nutritional composition	796:822	The nutritional composition	796:822	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	5	28	contain	had	1074:1076	arg2	%					1107:1107	40-85%	1102:1107	40-85%	1102:1107	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	28	contain	had	1074:1076	arg2	value					1125:1129	biological value	1114:1129	biological value (>93%)	1114:1136	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	28	contain	had	1074:1076	arg2	%					1135:1135	>93%	1132:1135	>93%	1132:1135	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	28	contain	had	1074:1076	arg1	The					1066:1068	The	1066:1068	The	1066:1068	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	5	28	contain	had	1074:1076	arg2	content					1093:1099	a high protein content	1078:1099	a high protein content (40-85%)	1078:1108	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	1	29	theme	low	367:369	arg1	price					371:375	low price	367:375	low price	367:375	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	7	30	theme	amine	1318:1322	arg1	groups					1324:1329	the amine groups I and II	1314:1338	the amine groups I and II responsible for the protein-protein interaction	1314:1386	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	30	theme	amine	1318:1322	arg1	II					1337:1338	II	1337:1338	II	1337:1338	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	30	theme	amine	1318:1322	arg1	I					1331:1331	I	1331:1331	I	1331:1331	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	31	theme	protein-carbohydrate	1470:1489	arg1	interactions					1491:1502	the protein-carbohydrate interactions	1466:1502	the protein-carbohydrate interactions	1466:1502	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	2	32	from	type	572:575	arg1	properties					640:649	these properties	634:649	these properties	634:649	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	4	33	theme	Scanning	917:924	arg1	DSC					939:941	DSC	939:941	DSC	939:941	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	4	33	theme	Scanning	917:924	arg1	Calorimetry					926:936	Differential Scanning Calorimetry	904:936	Differential Scanning Calorimetry (DSC)	904:942	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	2	34	with	interaction	413:423	arg1	starch					459:464	starch	459:464	starch	459:464	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	2	35	theme	animal	440:445	arg1	origin					447:452	animal origin	440:452	animal origin	440:452	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	2	36	from	interactions	601:612	arg1	properties					640:649	these properties	634:649	these properties	634:649	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	0	37	theme	Squid	15:19	arg1	Protein					45:51	Squid (Dosidicus giga) Mantle Protein	15:51	Squid (Dosidicus giga) Mantle Protein	15:51	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	1	38	theme	vegetable	216:224	arg1	sources					226:232	vegetable sources	216:232	vegetable sources	216:232	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	0	39	theme	Starch	88:93	arg1	Mixtures					60:67	a Mixtures	58:67	a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC	58:148	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	0	40	from	Mixtures	60:67	arg1	Snack					110:114	an Extruded Snack	98:114	an Extruded Snack	98:114	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	4	41	theme	protein-starch	980:993	arg1	complexes					995:1003	protein-starch complexes	980:1003	protein-starch complexes	980:1003	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	0	42	theme	giga	32:35	arg1	Protein					45:51	Squid (Dosidicus giga) Mantle Protein	15:51	Squid (Dosidicus giga) Mantle Protein	15:51	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	0	43	theme	Extruded	101:108	arg1	Snack					110:114	an Extruded Snack	98:114	an Extruded Snack	98:114	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	1	44	dep	extrusion	186:194	arg1	SEE					197:199	SEE	197:199	SEE	197:199	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	0	45	theme	Dosidicus	22:30	arg1	Protein					45:51	Squid (Dosidicus giga) Mantle Protein	15:51	Squid (Dosidicus giga) Mantle Protein	15:51	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	7	46	dep	groups	1324:1329	arg1	groups					1324:1329	the amine groups I and II	1314:1338	the amine groups I and II responsible for the protein-protein interaction	1314:1386	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	46	dep	groups	1324:1329	arg1	II					1337:1338	II	1337:1338	II	1337:1338	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	7	46	dep	groups	1324:1329	arg1	I					1331:1331	I	1331:1331	I	1331:1331	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	2	47	from	effect	624:629	arg1	properties					640:649	these properties	634:649	these properties	634:649	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	0	48	theme	Mantle	38:43	arg1	Protein					45:51	Squid (Dosidicus giga) Mantle Protein	15:51	Squid (Dosidicus giga) Mantle Protein	15:51	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	2	49	from	properties	640:649	arg1	type					572:575	the type	568:575	the type of protein-carbohydrate interactions and their effect on these properties	568:649	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	1	50	theme	high	381:384	arg1	availability					386:397	high availability	381:397	high availability	381:397	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	6	51	theme	Tm	1206:1207	arg1	values					1209:1214	the Tm values	1202:1214	the Tm values in extruded samples	1202:1234	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	6	51	theme	Tm	1206:1207	arg1	proportional					1249:1260	proportional	1249:1260	proportional	1249:1260	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	5	52	dep	The	1066:1068	arg1	SEE					1070:1072	SEE	1070:1072	SEE	1070:1072	The SEE had a high protein content (40-85%) and biological value (>93%).					
33917637	6	53	from	values	1209:1214	arg1	samples					1228:1234	extruded samples	1219:1234	extruded samples	1219:1234	The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content.					
33917637	3	54	theme	carbohydrates	728:740	arg1	interaction					700:710	the interaction	696:710	the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn	696:793	The objective of this research was to study the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn.					
33917637	4	55	theme	Differential	904:915	arg1	DSC					939:941	DSC	939:941	DSC	939:941	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	4	55	theme	Differential	904:915	arg1	Calorimetry					926:936	Differential Scanning Calorimetry	904:936	Differential Scanning Calorimetry (DSC)	904:942	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	0	56	with	Interaction	0:10	arg1	Mixtures					60:67	a Mixtures	58:67	a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC	58:148	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	1	57	theme	snacks	167:172	arg1	majority					155:162	The majority	151:162	The majority of snacks expanded by extrusion (SEE)	151:200	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	2	58	theme	origin	447:452	arg1	proteins					428:435	proteins	428:435	proteins of animal origin	428:452	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	4	59	theme	responsible	1030:1040	arg1	regions					1022:1028	the possible regions	1009:1028	the possible regions responsible for their interactions	1009:1063	The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein-starch complexes and the possible regions responsible for their interactions.					
33917637	0	60	dep	Snack	110:114	arg1	Characterized					120:132	Characterized	120:132	Characterized by FTIR and DSC	120:148	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	3	61	theme	proteins	715:722	arg1	interaction					700:710	the interaction	696:710	the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn	696:793	The objective of this research was to study the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn.					
33917637	7	62	theme	O-glucosidic	1406:1417	arg1	bonds					1419:1423	the O-glucosidic bonds	1402:1423	the O-glucosidic bonds	1402:1423	The extrusion process reduced the amine groups I and II responsible for the protein-protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein-carbohydrate interactions.					
33917637	0	63	theme	Protein	45:51	arg1	Interaction					0:10	Interaction	0:10	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.	0:149	Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC.					
33917637	1	64	theme	nutritional	252:262	arg1	content					264:270	their nutritional content	246:270	their nutritional content	246:270	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
33917637	3	65	theme	research	674:681	arg1	objective					656:664	The objective	652:664	The objective of this research	652:681	The objective of this research was to study the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn.					
33917637	2	66	theme	interactions	601:612	arg1	type					572:575	the type	568:575	the type of protein-carbohydrate interactions and their effect on these properties	568:649	However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein-carbohydrate interactions and their effect on these properties.					
33917637	1	67	dep	squid	309:313	arg1	gigas					326:330	Dosidicus gigas	316:330	Dosidicus gigas	316:330	The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability.					
32363766	8	0	theme	P.	1437:1438	arg1	treatment					1457:1465	The P. pentosaceus LI05 treatment	1433:1465	The P. pentosaceus LI05 treatment	1433:1465	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	5	1	theme	disease	868:874	arg1	DAI					892:894	DAI	892:894	DAI	892:894	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	1	theme	disease	868:874	arg1	index					885:889	disease activity index	868:889	disease activity index (DAI) scores	868:902	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	6	2	theme	short-chain	1088:1098	arg1	SCFA					1112:1115	SCFA	1112:1115	SCFA	1112:1115	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	6	2	theme	short-chain	1088:1098	arg1	acid					1106:1109	short-chain fatty acid	1088:1109	short-chain fatty acid (SCFA) production	1088:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	5	3	theme	activity	876:883	arg1	DAI					892:894	DAI	892:894	DAI	892:894	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	3	theme	activity	876:883	arg1	index					885:889	disease activity index	868:889	disease activity index (DAI) scores	868:902	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	1	4	theme	inflammatory	250:261	arg1	IBD					278:280	IBD	278:280	IBD	278:280	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	1	4	theme	inflammatory	250:261	arg1	disease					269:275	inflammatory bowel disease	250:275	inflammatory bowel disease (IBD)	250:281	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	8	5	theme	epithelial	1532:1541	arg1	integrity					1543:1551	the intestinal epithelial integrity	1517:1551	the intestinal epithelial integrity	1517:1551	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	6	6	theme	P.	1163:1164	arg1	LI05					1178:1181	P. pentosaceus LI05	1163:1181	P. pentosaceus LI05	1163:1181	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	1	7	theme	bowel	263:267	arg1	IBD					278:280	IBD	278:280	IBD	278:280	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	1	7	theme	bowel	263:267	arg1	disease					269:275	inflammatory bowel disease	250:275	inflammatory bowel disease (IBD)	250:281	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	0	8	theme	gut	102:104	arg1	microbiota					106:115	the gut microbiota	98:115	the gut microbiota	98:115	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	1	9	theme	disease	269:275	arg1	progression					235:245	progression	235:245	progression	235:245	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	1	9	theme	disease	269:275	arg1	pathogenesis					218:229	pathogenesis	218:229	pathogenesis	218:229	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	0	10	theme	short-chain	121:131	arg1	acid					139:142	short-chain fatty acid	121:142	short-chain fatty acid levels	121:149	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	8	11	dep	P.	1437:1438	arg1	pentosaceus					1440:1450	pentosaceus	1440:1450	pentosaceus	1440:1450	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	9	12	theme	potential	1702:1710	arg1	preparation					1712:1722	potential preparation	1702:1722	potential preparation	1702:1722	Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.					
32363766	9	12	theme	potential	1702:1710	arg1	LI05					1677:1680	P. pentosaceus LI05	1662:1680	P. pentosaceus LI05	1662:1680	Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.					
32363766	5	13	theme	P.	786:787	arg1	LI05					801:804	P. pentosaceus LI05	786:804	P. pentosaceus LI05	786:804	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	4	14	theme	drinking	759:766	arg1	water					768:772	drinking water	759:772	drinking water for 7 days	759:783	Mice were administered P. pentosaceus LI05 or phosphate-buffered saline once daily by oral gavage for 14 days, and colitis was induced by providing mice 2% DSS-containing drinking water for 7 days.					
32363766	5	15	theme	body	850:853	arg1	shortening					918:927	colon length shortening	905:927	colon length shortening	905:927	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	15	theme	body	850:853	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	15	theme	body	850:853	arg1	scores					897:902	disease activity index (DAI) scores	868:902	disease activity index (DAI) scores	868:902	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	15	theme	body	850:853	arg1	levels					987:992	the proinflammatory cytokine levels	958:992	the proinflammatory cytokine levels	958:992	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	15	theme	body	850:853	arg1	permeability					941:952	intestinal permeability	930:952	intestinal permeability	930:952	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	6	16	theme	altered	1024:1030	arg1	composition					1047:1057	a significantly altered gut microbiota composition	1008:1057	a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production	1008:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	2	17	from	effect	470:475	arg1	IBD					480:482	IBD	480:482	IBD	480:482	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	5	18	theme	pentosaceus	789:799	arg1	LI05					801:804	P. pentosaceus LI05	786:804	P. pentosaceus LI05	786:804	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	1	19	theme	gut	173:175	arg1	microbiota					177:186	The gut microbiota	169:186	The gut microbiota	169:186	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	0	20	theme	pentosaceus	12:22	arg1	LI05					24:27	Pediococcus pentosaceus LI05	0:27	Pediococcus pentosaceus LI05	0:27	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	6	21	theme	increased	1064:1072	arg1	diversity					1074:1082	increased diversity	1064:1082	increased diversity	1064:1082	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	2	22	theme	pentosaceus	310:320	arg1	LI05					322:325	The bacterium Pediococcus pentosaceus LI05	284:325	The bacterium Pediococcus pentosaceus LI05	284:325	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	4	23	theme	phosphate-buffered	634:651	arg1	saline					653:658	phosphate-buffered saline	634:658	phosphate-buffered saline	634:658	Mice were administered P. pentosaceus LI05 or phosphate-buffered saline once daily by oral gavage for 14 days, and colitis was induced by providing mice 2% DSS-containing drinking water for 7 days.					
32363766	0	24	theme	Pediococcus	0:10	arg1	LI05					24:27	Pediococcus pentosaceus LI05	0:27	Pediococcus pentosaceus LI05	0:27	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	9	25	theme	P.	1662:1663	arg1	preparation					1712:1722	potential preparation	1702:1722	potential preparation	1702:1722	Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.					
32363766	9	25	theme	P.	1662:1663	arg1	LI05					1677:1680	P. pentosaceus LI05	1662:1680	P. pentosaceus LI05	1662:1680	Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.					
32363766	2	26	theme	Pediococcus	298:308	arg1	LI05					322:325	The bacterium Pediococcus pentosaceus LI05	284:325	The bacterium Pediococcus pentosaceus LI05	284:325	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	7	27	theme	P.	1280:1281	arg1	mice					1308:1311	the P. pentosaceus LI05-treated mice	1276:1311	the P. pentosaceus LI05-treated mice	1276:1311	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	2	28	theme	epithelial	379:388	arg1	integrity					390:398	the gut epithelial integrity	371:398	the gut epithelial integrity	371:398	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	7	29	theme	SCFA	1420:1423	arg1	levels					1425:1430	SCFA levels	1420:1430	SCFA levels	1420:1430	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	0	30	theme	acid	139:142	arg1	levels					144:149	short-chain fatty acid levels	121:149	short-chain fatty acid levels	121:149	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	7	31	theme	colitis	1349:1355	arg1	indices					1357:1363	colitis indices	1349:1363	colitis indices	1349:1363	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	2	32	theme	bacterium	288:296	arg1	LI05					322:325	The bacterium Pediococcus pentosaceus LI05	284:325	The bacterium Pediococcus pentosaceus LI05	284:325	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	6	33	theme	gut	1032:1034	arg1	composition					1047:1057	a significantly altered gut microbiota composition	1008:1057	a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production	1008:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	2	34	theme	gut	431:433	arg1	immunity					421:428	the host immunity	412:428	the host immunity	412:428	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	2	34	theme	gut	431:433	arg1	microbiota					435:444	gut microbiota	431:444	gut microbiota	431:444	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	0	35	theme	fatty	133:137	arg1	acid					139:142	short-chain fatty acid	121:142	short-chain fatty acid levels	121:149	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	6	36	with	composition	1047:1057	arg1	production					1118:1127	short-chain fatty acid (SCFA) production	1088:1127	short-chain fatty acid (SCFA) production	1088:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	6	36	with	composition	1047:1057	arg1	diversity					1074:1082	increased diversity	1064:1082	increased diversity	1064:1082	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	5	37	theme	length	911:916	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	37	theme	length	911:916	arg1	shortening					918:927	colon length shortening	905:927	colon length shortening	905:927	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	3	38	theme	present	505:511	arg1	study					513:517	The present study	501:517	The present study	501:517	The present study aimed to investigate the role and mechanisms of P. pentosaceus LI05.					
32363766	8	39	theme	gut	1596:1598	arg1	profiles					1586:1593	the immunological profiles	1568:1593	the immunological profiles	1568:1593	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	8	39	theme	gut	1596:1598	arg1	microbiome					1600:1609	gut microbiome	1596:1609	gut microbiome	1596:1609	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	1	40	theme	key	204:206	arg1	factor					208:213	a key factor	202:213	a key factor	202:213	The gut microbiota is considered a key factor in pathogenesis and progression of inflammatory bowel disease (IBD).					
32363766	3	41	theme	LI05	582:585	arg1	mechanisms					553:562	mechanisms	553:562	mechanisms	553:562	The present study aimed to investigate the role and mechanisms of P. pentosaceus LI05.					
32363766	3	41	theme	LI05	582:585	arg1	role					544:547	role	544:547	role	544:547	The present study aimed to investigate the role and mechanisms of P. pentosaceus LI05.					
32363766	5	42	theme	weight	855:860	arg1	shortening					918:927	colon length shortening	905:927	colon length shortening	905:927	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	42	theme	weight	855:860	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	42	theme	weight	855:860	arg1	scores					897:902	disease activity index (DAI) scores	868:902	disease activity index (DAI) scores	868:902	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	42	theme	weight	855:860	arg1	levels					987:992	the proinflammatory cytokine levels	958:992	the proinflammatory cytokine levels	958:992	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	42	theme	weight	855:860	arg1	permeability					941:952	intestinal permeability	930:952	intestinal permeability	930:952	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	2	43	theme	host	416:419	arg1	metabolism					450:459	metabolism	450:459	metabolism	450:459	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	2	43	theme	host	416:419	arg1	immunity					421:428	the host immunity	412:428	the host immunity	412:428	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	2	43	theme	host	416:419	arg1	microbiota					435:444	gut microbiota	431:444	gut microbiota	431:444	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	0	44	theme	DSS-induced	40:50	arg1	colitis					52:58	DSS-induced colitis	40:58	DSS-induced colitis	40:58	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	8	45	theme	metabolite	1615:1624	arg1	profiles					1586:1593	the immunological profiles	1568:1593	the immunological profiles	1568:1593	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	8	45	theme	metabolite	1615:1624	arg1	composition					1626:1636	metabolite composition	1615:1636	metabolite composition	1615:1636	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	7	46	theme	barrier	1400:1406	arg1	markers					1408:1414	gut barrier markers	1396:1414	gut barrier markers	1396:1414	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	7	47	theme	gut	1396:1398	arg1	markers					1408:1414	gut barrier markers	1396:1414	gut barrier markers	1396:1414	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	9	48	theme	pentosaceus	1665:1675	arg1	preparation					1712:1722	potential preparation	1702:1722	potential preparation	1702:1722	Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.					
32363766	9	48	theme	pentosaceus	1665:1675	arg1	LI05					1677:1680	P. pentosaceus LI05	1662:1680	P. pentosaceus LI05	1662:1680	Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.					
32363766	7	49	theme	pentosaceus	1283:1293	arg1	mice					1308:1311	the P. pentosaceus LI05-treated mice	1276:1311	the P. pentosaceus LI05-treated mice	1276:1311	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	0	50	theme	mouse	156:160	arg1	model					162:166	a mouse model	154:166	a mouse model	154:166	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	3	51	theme	P.	567:568	arg1	LI05					582:585	P. pentosaceus LI05	567:585	P. pentosaceus LI05	567:585	The present study aimed to investigate the role and mechanisms of P. pentosaceus LI05.					
32363766	6	52	theme	fatty	1100:1104	arg1	SCFA					1112:1115	SCFA	1112:1115	SCFA	1112:1115	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	6	52	theme	fatty	1100:1104	arg1	acid					1106:1109	short-chain fatty acid	1088:1109	short-chain fatty acid (SCFA) production	1088:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	5	53	theme	index	885:889	arg1	scores					897:902	disease activity index (DAI) scores	868:902	disease activity index (DAI) scores	868:902	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	53	theme	index	885:889	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	8	54	theme	immunological	1572:1584	arg1	profiles					1586:1593	the immunological profiles	1568:1593	the immunological profiles	1568:1593	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	8	54	theme	immunological	1572:1584	arg1	microbiome					1600:1609	gut microbiome	1596:1609	gut microbiome	1596:1609	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	8	54	theme	immunological	1572:1584	arg1	composition					1626:1636	metabolite composition	1615:1636	metabolite composition	1615:1636	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	4	55	dep	P.	611:612	arg1	pentosaceus					614:624	pentosaceus	614:624	pentosaceus	614:624	Mice were administered P. pentosaceus LI05 or phosphate-buffered saline once daily by oral gavage for 14 days, and colitis was induced by providing mice 2% DSS-containing drinking water for 7 days.					
32363766	3	56	theme	pentosaceus	570:580	arg1	LI05					582:585	P. pentosaceus LI05	567:585	P. pentosaceus LI05	567:585	The present study aimed to investigate the role and mechanisms of P. pentosaceus LI05.					
32363766	5	57	from	colitis	818:824	arg1	mice					829:832	mice	829:832	mice	829:832	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	2	58	theme	host	338:341	arg1	inflammation					343:354	host inflammation	338:354	host inflammation	338:354	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	6	59	theme	pentosaceus	1166:1176	arg1	LI05					1178:1181	P. pentosaceus LI05	1163:1181	P. pentosaceus LI05	1163:1181	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	8	60	theme	intestinal	1478:1487	arg1	inflammation					1489:1500	intestinal inflammation	1478:1500	intestinal inflammation	1478:1500	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	5	61	theme	colon	905:909	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	61	theme	colon	905:909	arg1	shortening					918:927	colon length shortening	905:927	colon length shortening	905:927	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	6	62	located	observed	1133:1140	arg2	composition					1047:1057	a significantly altered gut microbiota composition	1008:1057	a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production	1008:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	6	62	located	observed	1133:1140	arg1	mice					1145:1148	mice	1145:1148	mice treated with P. pentosaceus LI05	1145:1181	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	8	63	theme	LI05	1452:1455	arg1	treatment					1457:1465	The P. pentosaceus LI05 treatment	1433:1465	The P. pentosaceus LI05 treatment	1433:1465	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	6	64	theme	microbiota	1036:1045	arg1	composition					1047:1057	a significantly altered gut microbiota composition	1008:1057	a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production	1008:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	6	65	theme	acid	1106:1109	arg1	production					1118:1127	short-chain fatty acid (SCFA) production	1088:1127	short-chain fatty acid (SCFA) production	1088:1127	Furthermore, a significantly altered gut microbiota composition with increased diversity and short-chain fatty acid (SCFA) production was observed in mice treated with P. pentosaceus LI05.					
32363766	5	66	theme	intestinal	930:939	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	66	theme	intestinal	930:939	arg1	permeability					941:952	intestinal permeability	930:952	intestinal permeability	930:952	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	67	theme	proinflammatory	962:976	arg1	cytokine					978:985	the proinflammatory cytokine	958:985	the proinflammatory cytokine levels	958:992	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	3	68	dep	role	544:547	arg1	the					540:542	the	540:542	the	540:542	The present study aimed to investigate the role and mechanisms of P. pentosaceus LI05.					
32363766	2	69	theme	gut	375:377	arg1	integrity					390:398	the gut epithelial integrity	371:398	the gut epithelial integrity	371:398	The bacterium Pediococcus pentosaceus LI05 alleviated host inflammation by maintaining the gut epithelial integrity, modulating the host immunity, gut microbiota and metabolism, but its effect on IBD remains unclear.					
32363766	7	70	theme	LI05-treated	1295:1306	arg1	mice					1308:1311	the P. pentosaceus LI05-treated mice	1276:1311	the P. pentosaceus LI05-treated mice	1276:1311	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
32363766	4	71	theme	P.	611:612	arg1	LI05					626:629	P. pentosaceus LI05	611:629	P. pentosaceus LI05	611:629	Mice were administered P. pentosaceus LI05 or phosphate-buffered saline once daily by oral gavage for 14 days, and colitis was induced by providing mice 2% DSS-containing drinking water for 7 days.					
32363766	0	72	theme	immunological	74:86	arg1	profiles					88:95	immunological profiles	74:95	immunological profiles	74:95	Pediococcus pentosaceus LI05 alleviates DSS-induced colitis by modulating immunological profiles, the gut microbiota and short-chain fatty acid levels in a mouse model.					
32363766	8	73	theme	intestinal	1521:1530	arg1	integrity					1543:1551	the intestinal epithelial integrity	1517:1551	the intestinal epithelial integrity	1517:1551	The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition.					
32363766	5	74	theme	cytokine	978:985	arg1	loss					862:865	the body weight loss	846:865	the body weight loss	846:865	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	5	74	theme	cytokine	978:985	arg1	levels					987:992	the proinflammatory cytokine levels	958:992	the proinflammatory cytokine levels	958:992	P. pentosaceus LI05 ameliorated colitis in mice and reduced the body weight loss, disease activity index (DAI) scores, colon length shortening, intestinal permeability and the proinflammatory cytokine levels.					
32363766	4	75	theme	oral	674:677	arg1	gavage					679:684	oral gavage	674:684	oral gavage for 14 days	674:696	Mice were administered P. pentosaceus LI05 or phosphate-buffered saline once daily by oral gavage for 14 days, and colitis was induced by providing mice 2% DSS-containing drinking water for 7 days.					
32363766	7	76	theme	Several	1184:1190	arg1	genera					1192:1197	Several genera	1184:1197	Several genera	1184:1197	Several genera, including Akkermansia and Faecalibacterium, were differentially enriched in the P. pentosaceus LI05-treated mice and were negatively correlated with colitis indices and positively correlated with gut barrier markers and SCFA levels.					
31978467	4	0	theme	mechanical	548:557	arg1	properties					559:568	The mechanical properties	544:568	The mechanical properties of the composite film	544:590	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	6	1	theme	CMC	959:961	arg1	film					963:966	The CMC film	955:966	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0)	955:1040	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	6	1	theme	CMC	959:961	arg1	optimal					1046:1052	optimal	1046:1052	optimal	1046:1052	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	3	2	theme	water	512:516	arg1	properties					532:541	increased water vapor barrier properties	502:541	increased water vapor barrier properties	502:541	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	0	3	theme	zinc	113:116	arg1	oxide					118:122	zinc oxide	113:122	zinc oxide	113:122	Carboxymethyl cellulose-based antioxidant and antimicrobial active packaging film incorporated with curcumin and zinc oxide.					
31978467	3	4	theme	vapor	518:522	arg1	properties					532:541	increased water vapor barrier properties	502:541	increased water vapor barrier properties	502:541	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	4	5	theme	combined	715:722	arg1	addition					724:731	the combined addition	711:731	the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%)	711:769	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	4	6	theme	curcumin	639:646	arg1	addition					621:628	the addition	617:628	the addition of 1 wt% curcumin	617:646	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	4	7	theme	1 wt	633:636	arg1	curcumin					639:646	1 wt% curcumin	633:646	1 wt% curcumin	633:646	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	4	8	theme	ZnO	759:761	arg1	addition					724:731	the combined addition	711:731	the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%)	711:769	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	3	9	theme	increased	502:510	arg1	properties					532:541	increased water vapor barrier properties	502:541	increased water vapor barrier properties	502:541	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	0	10	theme	cellulose-based	14:28	arg1	antioxidant					30:40	Carboxymethyl cellulose-based antioxidant	0:40	Carboxymethyl cellulose-based antioxidant	0:40	Carboxymethyl cellulose-based antioxidant and antimicrobial active packaging film incorporated with curcumin and zinc oxide.					
31978467	4	11	theme	ZnO	690:692	arg1	addition					672:679	the addition	668:679	the addition of 1 wt% ZnO	668:692	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	6	12	theme	ZnO	989:991	arg1	addition					977:984	the addition	973:984	the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0)	973:1040	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	3	13	theme	CMC/curcumin/ZnO	366:381	arg1	films					393:397	The CMC/curcumin/ZnO composite films	362:397	The CMC/curcumin/ZnO composite films	362:397	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	0	14	theme	Carboxymethyl	0:12	arg1	antioxidant					30:40	Carboxymethyl cellulose-based antioxidant	0:40	Carboxymethyl cellulose-based antioxidant	0:40	Carboxymethyl cellulose-based antioxidant and antimicrobial active packaging film incorporated with curcumin and zinc oxide.					
31978467	4	15	theme	%	688:688	arg1	ZnO					690:692	1 wt% ZnO	684:692	1 wt% ZnO	684:692	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	4	16	theme	%	637:637	arg1	curcumin					639:646	1 wt% curcumin	633:646	1 wt% curcumin	633:646	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	4	17	theme	composite	577:585	arg1	film					587:590	the composite film	573:590	the composite film	573:590	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	3	18	theme	barrier	524:530	arg1	properties					532:541	increased water vapor barrier properties	502:541	increased water vapor barrier properties	502:541	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	4	19	dep	while	649:653	arg1	unchanged					655:663	unchanged	655:663	unchanged	655:663	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	4	19	dep	while	649:653	arg1	improved					699:706	improved	699:706	improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%)	699:769	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	6	20	theme	functional	1068:1077	arg1	films					1079:1083	functional films	1068:1083	functional films	1068:1083	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	6	21	theme	curcumin	1005:1012	arg1	addition					977:984	the addition	973:984	the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0)	973:1040	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	0	22	theme	active	60:65	arg1	film					77:80	antimicrobial active packaging film	46:80	antimicrobial active packaging film	46:80	Carboxymethyl cellulose-based antioxidant and antimicrobial active packaging film incorporated with curcumin and zinc oxide.					
31978467	5	23	theme	antioxidant	823:833	arg1	activity					835:842	strong antioxidant activity	816:842	strong antioxidant activity	816:842	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	3	24	theme	composite	383:391	arg1	films					393:397	The CMC/curcumin/ZnO composite films	362:397	The CMC/curcumin/ZnO composite films	362:397	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	0	25	theme	antimicrobial	46:58	arg1	film					77:80	antimicrobial active packaging film	46:80	antimicrobial active packaging film	46:80	Carboxymethyl cellulose-based antioxidant and antimicrobial active packaging film incorporated with curcumin and zinc oxide.					
31978467	5	26	theme	CMC	772:774	arg1	films					776:780	CMC films	772:780	CMC films incorporated with curcumin	772:807	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	1	27	dep	curcumin	209:216	arg1	%					233:233	0.5 and 1.0 wt%	219:233	0.5 and 1.0 wt% based on CMC	219:246	Carboxymethyl cellulose (CMC)-based functional films were prepared by incorporating curcumin (0.5 and 1.0 wt% based on CMC), ZnO (1 wt% based on CMC).					
31978467	3	28	theme	increased	420:428	arg1	UV-barrier					430:439	significantly increased UV-barrier	406:439	significantly increased UV-barrier	406:439	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	5	29	theme	strong	816:821	arg1	activity					835:842	strong antioxidant activity	816:842	strong antioxidant activity	816:842	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	0	30	theme	packaging	67:75	arg1	film					77:80	antimicrobial active packaging film	46:80	antimicrobial active packaging film	46:80	Carboxymethyl cellulose-based antioxidant and antimicrobial active packaging film incorporated with curcumin and zinc oxide.					
31978467	1	31	dep	ZnO	250:252	arg1	%					259:259	1 wt%	255:259	1 wt% based on CMC	255:272	Carboxymethyl cellulose (CMC)-based functional films were prepared by incorporating curcumin (0.5 and 1.0 wt% based on CMC), ZnO (1 wt% based on CMC).					
31978467	6	32	theme	antibacterial	1106:1118	arg1	properties					1120:1129	antibacterial properties	1106:1129	antibacterial properties	1106:1129	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	3	33	theme	transparency	467:478	arg1	sacrifice					454:462	much sacrifice	449:462	much sacrifice of transparency	449:478	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	5	34	theme	pathogenic	934:943	arg1	bacteria					945:952	foodborne pathogenic bacteria	924:952	foodborne pathogenic bacteria	924:952	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	6	35	with	film	963:966	arg1	addition					977:984	the addition	973:984	the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0)	973:1040	The CMC film with the addition of ZnO (1 wt%) and curcumin (1 wt%) (CMC/Cur1.0/ZnO1.0) was optimal for producing functional films with antioxidant and antibacterial properties.					
31978467	3	36	theme	much	449:452	arg1	sacrifice					454:462	much sacrifice	449:462	much sacrifice of transparency	449:478	The CMC/curcumin/ZnO composite films showed significantly increased UV-barrier without much sacrifice of transparency, and they also showed increased water vapor barrier properties.					
31978467	4	37	theme	film	587:590	arg1	properties					559:568	The mechanical properties	544:568	The mechanical properties of the composite film	544:590	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	5	38	theme	antimicrobial	893:905	arg1	activity					907:914	strong antimicrobial activity	886:914	strong antimicrobial activity against foodborne pathogenic bacteria	886:952	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	5	39	theme	strong	886:891	arg1	activity					907:914	strong antimicrobial activity	886:914	strong antimicrobial activity against foodborne pathogenic bacteria	886:952	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	4	40	theme	1 wt	684:687	arg1	ZnO					690:692	1 wt% ZnO	684:692	1 wt% ZnO	684:692	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	2	41	theme	CMC	352:354	arg1	film					356:359	the CMC film	348:359	the CMC film	348:359	The FE-SEM showed that the ZnO and curcumin were uniformly dispersed in the CMC film.					
31978467	5	42	theme	foodborne	924:932	arg1	bacteria					945:952	foodborne pathogenic bacteria	924:952	foodborne pathogenic bacteria	924:952	CMC films incorporated with curcumin showed strong antioxidant activity, while films incorporated with ZnO showed strong antimicrobial activity against foodborne pathogenic bacteria.					
31978467	1	43	theme	-based	154:159	arg1	films					172:176	Carboxymethyl cellulose (CMC)-based functional films	125:176	Carboxymethyl cellulose (CMC)-based functional films	125:176	Carboxymethyl cellulose (CMC)-based functional films were prepared by incorporating curcumin (0.5 and 1.0 wt% based on CMC), ZnO (1 wt% based on CMC).					
31978467	4	44	theme	curcumin	736:743	arg1	addition					724:731	the combined addition	711:731	the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%)	711:769	The mechanical properties of the composite film were slightly reduced by the addition of 1 wt% curcumin, while unchanged by the addition of 1 wt% ZnO, but improved by the combined addition of curcumin (0.5 wt%) and ZnO (1 wt%).					
31978467	1	45	theme	functional	161:170	arg1	films					172:176	Carboxymethyl cellulose (CMC)-based functional films	125:176	Carboxymethyl cellulose (CMC)-based functional films	125:176	Carboxymethyl cellulose (CMC)-based functional films were prepared by incorporating curcumin (0.5 and 1.0 wt% based on CMC), ZnO (1 wt% based on CMC).					
34398423	8	0	theme	low	1245:1247	arg1	cytotoxicity					1249:1260	low cytotoxicity	1245:1260	low cytotoxicity towards human erythrocytes	1245:1287	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	3	1	with	nanoparticles	454:466	arg1	properties					493:502	improved biological properties	473:502	improved biological properties	473:502	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	3	1	with	nanoparticles	454:466	arg1	toxicity					516:523	reduced toxicity	508:523	reduced toxicity	508:523	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	5	2	theme	569 nm	745:750	arg1	range					770:774	a zeta potential range	753:774	a zeta potential range of 38.8 mV	753:785	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	2	theme	569 nm	745:750	arg1	EE					818:819	EE	818:819	EE	818:819	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	2	theme	569 nm	745:750	arg1	efficiency					806:815	encapsulation efficiency	792:815	encapsulation efficiency (EE) of 85.58%	792:830	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	2	theme	569 nm	745:750	arg1	size					737:740	an average size	726:740	an average size of 569 nm	726:750	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	6	3	theme	cells	868:872	arg1	Treatment					833:841	Treatment	833:841	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides	833:903	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	2	4	theme	mammalian	367:375	arg1	cells					377:381	mammalian cells	367:381	mammalian cells	367:381	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	6	5	theme	surface	965:971	arg1	hydrophobicity					973:986	decreased cell surface hydrophobicity	950:986	decreased cell surface hydrophobicity (CSH)	950:992	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	6	5	theme	surface	965:971	arg1	CSH					989:991	CSH	989:991	CSH	989:991	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	4	6	theme	gelation	672:679	arg1	process					681:687	ionotropic gelation process	661:687	ionotropic gelation process	661:687	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	9	7	theme	medical	1558:1564	arg1	devices					1566:1572	medical devices	1558:1572	medical devices	1558:1572	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	6	8	theme	decreased	950:958	arg1	hydrophobicity					973:986	decreased cell surface hydrophobicity	950:986	decreased cell surface hydrophobicity (CSH)	950:992	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	6	8	theme	decreased	950:958	arg1	CSH					989:991	CSH	989:991	CSH	989:991	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	1	9	theme	therapeutic	189:199	arg1	potential					201:209	interesting therapeutic potential	177:209	interesting therapeutic potential	177:209	Cyclic lipopeptides produced by Bacillus species exhibit interesting therapeutic potential.					
34398423	6	10	theme	%	944:944	arg1	activity					927:934	anti-adhesive activity	913:934	anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH)	913:992	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	5	11	theme	potential	760:768	arg1	range					770:774	a zeta potential range	753:774	a zeta potential range of 38.8 mV	753:785	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	7	12	theme	IC50	1163:1166	arg1	values					1168:1173	respective IC50 values	1152:1173	respective IC50 values of 14.37 µg/mL and 22.45 µg/mL	1152:1204	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	9	13	theme	anti-infectious	1584:1598	arg1	activity					1600:1607	their anti-infectious activity	1578:1607	their anti-infectious activity against leishmania	1578:1626	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	8	14	theme	macrophage	1303:1312	arg1	line					1319:1322	macrophage cell line	1303:1322	Raw 264.7 macrophage cell line	1293:1322	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	8	15	theme	770 µg/mL	1364:1372	arg1	HC50					1340:1343	respective HC50 and LC50 values	1329:1359	HC50	1340:1343	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	8	15	theme	770 µg/mL	1364:1372	arg1	values					1354:1359	respective HC50 and LC50 values	1329:1359	values	1354:1359	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	2	16	theme	host	314:317	arg1	macromolecules					319:332	host macromolecules	314:332	host macromolecules	314:332	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	9	17	dep	C.	1529:1530	arg1	albicans					1532:1539	albicans	1532:1539	albicans	1532:1539	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	8	18	theme	234.56 µg/mL	1378:1389	arg1	HC50					1340:1343	respective HC50 and LC50 values	1329:1359	HC50	1340:1343	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	8	18	theme	234.56 µg/mL	1378:1389	arg1	values					1354:1359	respective HC50 and LC50 values	1329:1359	values	1354:1359	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	6	19	with	Treatment	833:841	arg1	lipopeptides					892:903	encapsulated lipopeptides	879:903	encapsulated lipopeptides	879:903	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	5	20	theme	encapsulation	792:804	arg1	EE					818:819	EE	818:819	EE	818:819	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	20	theme	encapsulation	792:804	arg1	efficiency					806:815	encapsulation efficiency	792:815	encapsulation efficiency (EE) of 85.58%	792:830	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	8	21	theme	respective	1329:1338	arg1	HC50					1340:1343	respective HC50 and LC50 values	1329:1359	HC50	1340:1343	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	7	22	theme	amastigote	1132:1141	arg1	forms					1143:1147	Leishmania (L.) major promastigote and amastigote forms	1093:1147	Leishmania (L.) major promastigote and amastigote forms	1093:1147	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	7	23	theme	promastigote	1115:1126	arg1	forms					1143:1147	Leishmania (L.) major promastigote and amastigote forms	1093:1147	Leishmania (L.) major promastigote and amastigote forms	1093:1147	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	1	24	theme	Cyclic	120:125	arg1	lipopeptides					127:138	Cyclic lipopeptides	120:138	Cyclic lipopeptides produced by Bacillus species	120:167	Cyclic lipopeptides produced by Bacillus species exhibit interesting therapeutic potential.					
34398423	2	25	theme	low	269:271	arg1	stability					273:281	their low stability	263:281	their low stability	263:281	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	1	26	theme	Bacillus	152:159	arg1	species					161:167	Bacillus species	152:167	Bacillus species	152:167	Cyclic lipopeptides produced by Bacillus species exhibit interesting therapeutic potential.					
34398423	2	27	with	interactions	296:307	arg1	macromolecules					319:332	host macromolecules	314:332	host macromolecules	314:332	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	4	28	theme	D	553:553	arg1	lipopeptides					555:566	Surfactin and bacillomycin D lipopeptides	526:566	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain	526:616	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	0	29	from	Effect	0:5	arg1	Adhesion					72:79	Candida albicans Adhesion	55:79	Candida albicans Adhesion	55:79	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	0	29	from	Effect	0:5	arg1	Growth					92:97	the Growth	88:97	the Growth of Leishmania major	88:117	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	5	30	theme	Nanoformulated	690:703	arg1	lipopeptides					705:716	Nanoformulated lipopeptides	690:716	Nanoformulated lipopeptides	690:716	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	3	31	theme	improved	473:480	arg1	properties					493:502	improved biological properties	473:502	improved biological properties	473:502	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	9	32	theme	potential	1441:1449	arg1	system					1460:1465	a potential delivery system	1439:1465	a potential delivery system of lipopeptides	1439:1481	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	9	32	theme	potential	1441:1449	arg1	lipopeptides					1409:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	3	33	theme	present	388:394	arg1	work					396:399	The present work	384:399	The present work	384:399	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	6	34	from	2000 µg/mL	1007:1016	arg1	%					1002:1002	25.53%	997:1002	25.53% at 2000 µg/mL	997:1016	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	3	35	theme	reduced	508:514	arg1	toxicity					516:523	reduced toxicity	508:523	reduced toxicity	508:523	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	0	36	theme	Lipopeptide-Loaded	10:27	arg1	Nanoparticles					38:50	Lipopeptide-Loaded Chitosan Nanoparticles	10:50	Lipopeptide-Loaded Chitosan Nanoparticles	10:50	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	3	37	theme	lipopeptide-loaded	426:443	arg1	nanoparticles					454:466	suitable lipopeptide-loaded chitosan nanoparticles	417:466	suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity	417:523	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	0	38	theme	Nanoparticles	38:50	arg1	Effect					0:5	Effect	0:5	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.	0:118	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	9	39	theme	lipopeptides	1470:1481	arg1	system					1460:1465	a potential delivery system	1439:1465	a potential delivery system of lipopeptides	1439:1481	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	9	39	theme	lipopeptides	1470:1481	arg1	lipopeptides					1409:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	4	40	theme	Bacillus	580:587	arg1	strain					611:616	Bacillus amyloliquefaciens B84 strain	580:616	Bacillus amyloliquefaciens B84 strain	580:616	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	0	41	theme	Candida	55:61	arg1	albicans					63:70	Candida albicans	55:70	Candida albicans Adhesion	55:79	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	4	42	theme	B84	607:609	arg1	strain					611:616	Bacillus amyloliquefaciens B84 strain	580:616	Bacillus amyloliquefaciens B84 strain	580:616	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	8	43	dep	Raw	1293:1295	arg1	line					1319:1322	macrophage cell line	1303:1322	Raw 264.7 macrophage cell line	1293:1322	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	2	44	theme	potential	345:353	arg1	toxicity					355:362	their potential toxicity	339:362	their potential toxicity to mammalian cells	339:381	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	9	45	theme	anti-adhesive	1500:1512	arg1	effect					1514:1519	their anti-adhesive effect	1494:1519	their anti-adhesive effect	1494:1519	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	6	46	theme	encapsulated	879:890	arg1	lipopeptides					892:903	encapsulated lipopeptides	879:903	encapsulated lipopeptides	879:903	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	7	47	theme	major	1109:1113	arg1	forms					1143:1147	Leishmania (L.) major promastigote and amastigote forms	1093:1147	Leishmania (L.) major promastigote and amastigote forms	1093:1147	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	8	48	theme	Nanoencapsulated	1207:1222	arg1	lipopeptides					1224:1235	Nanoencapsulated lipopeptides	1207:1235	Nanoencapsulated lipopeptides	1207:1235	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	7	49	theme	22.45 µg/mL	1194:1204	arg1	values					1168:1173	respective IC50 values	1152:1173	respective IC50 values of 14.37 µg/mL and 22.45 µg/mL	1152:1204	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	6	50	theme	hydrophobicity	973:986	arg1	activity					927:934	anti-adhesive activity	913:934	anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH)	913:992	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	7	51	theme	respective	1152:1161	arg1	values					1168:1173	respective IC50 values	1152:1173	respective IC50 values of 14.37 µg/mL and 22.45 µg/mL	1152:1204	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	2	52	with	toxicity	355:362	arg1	macromolecules					319:332	host macromolecules	314:332	host macromolecules	314:332	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	7	53	theme	14.37 µg/mL	1178:1188	arg1	values					1168:1173	respective IC50 values	1152:1173	respective IC50 values of 14.37 µg/mL and 22.45 µg/mL	1152:1204	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	6	54	theme	albicans	859:866	arg1	cells					868:872	Candida (C.) albicans cells	846:872	Candida (C.) albicans cells	846:872	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	6	55	theme	cell	960:963	arg1	hydrophobicity					973:986	decreased cell surface hydrophobicity	950:986	decreased cell surface hydrophobicity (CSH)	950:992	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	6	55	theme	cell	960:963	arg1	CSH					989:991	CSH	989:991	CSH	989:991	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	4	56	theme	ionotropic	661:670	arg1	process					681:687	ionotropic gelation process	661:687	ionotropic gelation process	661:687	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	7	57	dep	major	1109:1113	arg1	Leishmania					1093:1102	Leishmania	1093:1102	Leishmania	1093:1102	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	6	58	theme	C.	855:856	arg1	albicans					859:866	Candida (C.) albicans	846:866	Candida (C.) albicans cells	846:872	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	2	59	theme	undesirable	284:294	arg1	interactions					296:307	undesirable interactions	284:307	undesirable interactions with host macromolecules	284:332	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	1	60	theme	interesting	177:187	arg1	potential					201:209	interesting therapeutic potential	177:209	interesting therapeutic potential	177:209	Cyclic lipopeptides produced by Bacillus species exhibit interesting therapeutic potential.					
34398423	8	61	theme	human	1270:1274	arg1	erythrocytes					1276:1287	human erythrocytes	1270:1287	human erythrocytes	1270:1287	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	5	62	theme	average	729:735	arg1	size					737:740	an average size	726:740	an average size of 569 nm	726:750	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	6	63	theme	Candida	846:852	arg1	albicans					859:866	Candida (C.) albicans	846:866	Candida (C.) albicans cells	846:872	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	8	64	theme	LC50	1349:1352	arg1	values					1354:1359	respective HC50 and LC50 values	1329:1359	values	1354:1359	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	5	65	theme	38.8 mV	779:785	arg1	range					770:774	a zeta potential range	753:774	a zeta potential range of 38.8 mV	753:785	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	65	theme	38.8 mV	779:785	arg1	EE					818:819	EE	818:819	EE	818:819	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	65	theme	38.8 mV	779:785	arg1	efficiency					806:815	encapsulation efficiency	792:815	encapsulation efficiency (EE) of 85.58%	792:830	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	65	theme	38.8 mV	779:785	arg1	size					737:740	an average size	726:740	an average size of 569 nm	726:750	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	8	66	theme	cell	1314:1317	arg1	line					1319:1322	macrophage cell line	1303:1322	Raw 264.7 macrophage cell line	1293:1322	Nanoencapsulated lipopeptides exerted low cytotoxicity towards human erythrocytes and Raw 264.7 macrophage cell line with respective HC50 and LC50 values of 770 µg/mL and 234.56 µg/mL.					
34398423	5	67	theme	zeta	755:758	arg1	range					770:774	a zeta potential range	753:774	a zeta potential range of 38.8 mV	753:785	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	2	68	with	stability	273:281	arg1	macromolecules					319:332	host macromolecules	314:332	host macromolecules	314:332	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	7	69	theme	antileishmanial	1060:1074	arg1	activity					1076:1083	antileishmanial activity	1060:1083	antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms	1060:1147	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	5	70	theme	%	830:830	arg1	range					770:774	a zeta potential range	753:774	a zeta potential range of 38.8 mV	753:785	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	70	theme	%	830:830	arg1	EE					818:819	EE	818:819	EE	818:819	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	70	theme	%	830:830	arg1	efficiency					806:815	encapsulation efficiency	792:815	encapsulation efficiency (EE) of 85.58%	792:830	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	5	70	theme	%	830:830	arg1	size					737:740	an average size	726:740	an average size of 569 nm	726:750	Nanoformulated lipopeptides exhibit an average size of 569 nm, a zeta potential range of 38.8 mV, and encapsulation efficiency (EE) of 85.58%.					
34398423	9	71	theme	Nanoencapsulated	1392:1407	arg1	lipopeptides					1409:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	9	71	theme	Nanoencapsulated	1392:1407	arg1	system					1460:1465	a potential delivery system	1439:1465	a potential delivery system of lipopeptides	1439:1481	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	9	72	used	used	1431:1434	arg2	system					1460:1465	a potential delivery system	1439:1465	a potential delivery system of lipopeptides	1439:1481	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	9	72	used	used	1431:1434	arg2	lipopeptides					1409:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	7	73	theme	Nanoformulated	1019:1032	arg1	lipopeptides					1034:1045	Nanoformulated lipopeptides	1019:1045	Nanoformulated lipopeptides	1019:1045	Nanoformulated lipopeptides also induced antileishmanial activity against Leishmania (L.) major promastigote and amastigote forms at respective IC50 values of 14.37 µg/mL and 22.45 µg/mL.					
34398423	0	74	dep	major	113:117	arg1	Leishmania					102:111	Leishmania major	102:117	Leishmania major	102:117	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	4	75	theme	Surfactin	526:534	arg1	lipopeptides					555:566	Surfactin and bacillomycin D lipopeptides	526:566	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain	526:616	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	0	76	theme	major	113:117	arg1	Growth					92:97	the Growth	88:97	the Growth of Leishmania major	88:117	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	4	77	theme	bacillomycin	540:551	arg1	D					553:553	bacillomycin D	540:553	bacillomycin D	540:553	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	3	78	theme	biological	482:491	arg1	properties					493:502	improved biological properties	473:502	improved biological properties	473:502	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	0	79	theme	Chitosan	29:36	arg1	Nanoparticles					38:50	Lipopeptide-Loaded Chitosan Nanoparticles	10:50	Lipopeptide-Loaded Chitosan Nanoparticles	10:50	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	9	80	theme	delivery	1451:1458	arg1	system					1460:1465	a potential delivery system	1439:1465	a potential delivery system of lipopeptides	1439:1481	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	9	80	theme	delivery	1451:1458	arg1	lipopeptides					1409:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides	1392:1420	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
34398423	4	81	theme	chitosan	635:642	arg1	nanoparticles					644:656	chitosan nanoparticles	635:656	chitosan nanoparticles	635:656	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	3	82	theme	chitosan	445:452	arg1	nanoparticles					454:466	suitable lipopeptide-loaded chitosan nanoparticles	417:466	suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity	417:523	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	4	83	theme	amyloliquefaciens	589:605	arg1	strain					611:616	Bacillus amyloliquefaciens B84 strain	580:616	Bacillus amyloliquefaciens B84 strain	580:616	Surfactin and bacillomycin D lipopeptides produced by Bacillus amyloliquefaciens B84 strain were loaded onto chitosan nanoparticles by ionotropic gelation process.					
34398423	0	84	theme	albicans	63:70	arg1	Adhesion					72:79	Candida albicans Adhesion	55:79	Candida albicans Adhesion	55:79	Effect of Lipopeptide-Loaded Chitosan Nanoparticles on Candida albicans Adhesion and on the Growth of Leishmania major.					
34398423	6	85	theme	anti-adhesive	913:925	arg1	activity					927:934	anti-adhesive activity	913:934	anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH)	913:992	Treatment of Candida (C.) albicans cells with encapsulated lipopeptides induced anti-adhesive activity of 81.17% and decreased cell surface hydrophobicity (CSH) by 25.53% at 2000 µg/mL.					
34398423	3	86	theme	suitable	417:424	arg1	nanoparticles					454:466	suitable lipopeptide-loaded chitosan nanoparticles	417:466	suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity	417:523	The present work aims to develop suitable lipopeptide-loaded chitosan nanoparticles with improved biological properties and reduced toxicity.					
34398423	2	87	theme	clinical	227:234	arg1	use					236:238	their clinical use	221:238	their clinical use	221:238	However, their clinical use remains limited due to their low stability, undesirable interactions with host macromolecules, and their potential toxicity to mammalian cells.					
34398423	9	88	theme	C.	1529:1530	arg1	cells					1541:1545	C. albicans cells	1529:1545	C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania	1529:1626	Nanoencapsulated lipopeptides could be used as a potential delivery system of lipopeptides to improve their anti-adhesive effect against C. albicans cells colonizing medical devices and their anti-infectious activity against leishmania.					
33794993	8	0	theme	hAECs/CM	1204:1211	arg1	effect					1175:1180	the effect	1171:1180	the effect of SA-BG-encapsulated hAECs/CM on vascularization	1171:1230	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	12	1	theme	capillary	1857:1865	arg1	tube					1867:1870	the capillary tube	1853:1870	the capillary tube	1853:1870	Moreover, encapsulating hAECs could facilitate the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner.					
33794993	13	2	theme	pro-angiogenic	2015:2028	arg1	factors					2030:2036	pro-angiogenic factors	2015:2036	pro-angiogenic factors of hAECs	2015:2045	In addition, we found that SA-BG extracts significantly enhanced the viability of hAECs and stimulated the secretion of pro-angiogenic factors of hAECs.					
33794993	3	3	theme	composite	535:543	arg1	hydrogel					545:552	sodium alginate-bioglass (SA-BG) composite hydrogel	502:552	sodium alginate-bioglass (SA-BG) composite hydrogel	502:552	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	6	4	theme	SA-BG	944:948	arg1	hydrogel					960:967	SA-BG composite hydrogel	944:967	SA-BG composite hydrogel	944:967	hAECs or CM were harvested and encapsulated in SA-BG composite hydrogel, which were transplanted onto the injured ovaries of mice with POF.					
33794993	11	5	theme	hUVECs	1740:1745	arg1	formation					1727:1735	the tube formation	1718:1735	the tube formation of hUVECs	1718:1745	The further study showed that SA-BG significantly promoted the tube formation of hUVECs in vitro.					
33794993	1	6	from	function	240:247	arg1	POF					300:302	POF	300:302	POF	300:302	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	6	from	function	240:247	arg1	failure					291:297	chemotherapy-induced premature ovarian failure	252:297	chemotherapy-induced premature ovarian failure (POF)	252:303	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	9	7	theme	factors	1470:1476	arg1	secretion					1446:1454	the secretion	1442:1454	the secretion of angiogenic factors by hAECs	1442:1485	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	10	8	theme	ovarian	1625:1631	arg1	angiogenesis					1633:1644	ovarian angiogenesis	1625:1644	ovarian angiogenesis in POF mice	1625:1656	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	7	9	theme	morphological	1127:1139	arg1	methods					1141:1147	morphological methods	1127:1147	morphological methods	1127:1147	Follicle development, granulosa cell function, and ovarian angiogenesis were evaluated by morphological methods.					
33794993	15	10	theme	present	2236:2242	arg1	study					2244:2248	The present study	2232:2248	The present study	2232:2248	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	8	11	theme	human	1255:1259	arg1	hUVECs					1294:1299	hUVECs	1294:1299	hUVECs	1294:1299	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	8	11	theme	human	1255:1259	arg1	cells					1287:1291	human umbilical vein epithelial cells	1255:1291	human umbilical vein epithelial cells (hUVECs)	1255:1300	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	2	12	from	number	384:389	arg1	organs					444:449	target organs	437:449	target organs	437:449	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	2	13	theme	therapeutic	319:329	arg1	efficacy					331:338	the therapeutic efficacy	315:338	the therapeutic efficacy of hAECs	315:347	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	8	14	theme	vein	1271:1274	arg1	hUVECs					1294:1299	hUVECs	1294:1299	hUVECs	1294:1299	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	8	14	theme	vein	1271:1274	arg1	cells					1287:1291	human umbilical vein epithelial cells	1255:1291	human umbilical vein epithelial cells (hUVECs)	1255:1300	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	4	15	theme	therapeutic	673:683	arg1	potential					685:693	the therapeutic potential	669:693	the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF	669:802	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	8	16	theme	cells	1287:1291	arg1	formation					1242:1250	the tube formation	1233:1250	the tube formation of human umbilical vein epithelial cells (hUVECs)	1233:1300	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	3	17	theme	tissue	610:615	arg1	regeneration					617:628	tissue regeneration	610:628	tissue regeneration	610:628	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	7	18	theme	Follicle	1037:1044	arg1	development					1046:1056	Follicle development	1037:1056	Follicle development	1037:1056	Follicle development, granulosa cell function, and ovarian angiogenesis were evaluated by morphological methods.					
33794993	1	19	theme	Human	153:157	arg1	hAECs					186:190	hAECs	186:190	hAECs	186:190	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	19	theme	Human	153:157	arg1	cells					179:183	BACKGROUND Human amniotic epithelial cells	142:183	BACKGROUND Human amniotic epithelial cells (hAECs)	142:191	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	20	theme	epithelial	168:177	arg1	hAECs					186:190	hAECs	186:190	hAECs	186:190	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	20	theme	epithelial	168:177	arg1	cells					179:183	BACKGROUND Human amniotic epithelial cells	142:183	BACKGROUND Human amniotic epithelial cells (hAECs)	142:191	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	4	21	theme	conditioned	720:730	arg1	medium					732:737	hAEC-derived conditioned medium	707:737	hAEC-derived conditioned medium (CM)	707:742	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	4	21	theme	conditioned	720:730	arg1	CM					740:741	CM	740:741	CM	740:741	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	0	22	theme	ovarian	82:88	arg1	failure					90:96	premature ovarian failure	72:96	premature ovarian failure	72:96	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	9	23	theme	Cytokine	1326:1333	arg1	array					1335:1339	Cytokine array	1326:1339	Cytokine array	1326:1339	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	5	24	theme	METHODS	805:811	arg1	mice					821:824	METHODS C57BL/6 mice	805:824	METHODS C57BL/6 mice	805:824	METHODS C57BL/6 mice were intraperitoneally injected with chemotherapy drugs to induce POF.					
33794993	0	25	theme	angiogenic	113:122	arg1	secretion					131:139	angiogenic factor secretion	113:139	angiogenic factor secretion	113:139	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	6	26	theme	mice	1022:1025	arg1	ovaries					1011:1017	the injured ovaries	999:1017	the injured ovaries of mice with POF	999:1034	hAECs or CM were harvested and encapsulated in SA-BG composite hydrogel, which were transplanted onto the injured ovaries of mice with POF.					
33794993	10	27	theme	SA-BG-encapsulated	1519:1536	arg1	hAECs/CM					1538:1545	SA-BG-encapsulated hAECs/CM	1519:1545	SA-BG-encapsulated hAECs/CM	1519:1545	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	2	28	theme	grafted	420:426	arg1	hAECs					428:432	grafted hAECs	420:432	grafted hAECs	420:432	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	15	29	theme	granulosa	2389:2397	arg1	function					2404:2411	granulosa cell function	2389:2411	granulosa cell function	2389:2411	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	9	30	from	effects	1389:1395	arg1	secretion					1446:1454	the secretion	1442:1454	the secretion of angiogenic factors by hAECs	1442:1485	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	14	31	theme	BG	2161:2162	arg1	stimulation					2146:2156	stimulation	2146:2156	stimulation of BG	2146:2162	Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.					
33794993	13	32	theme	SA-BG	1922:1926	arg1	extracts					1928:1935	SA-BG extracts	1922:1935	SA-BG extracts	1922:1935	In addition, we found that SA-BG extracts significantly enhanced the viability of hAECs and stimulated the secretion of pro-angiogenic factors of hAECs.					
33794993	0	33	theme	Sodium	0:5	arg1	hAECs					38:42	Sodium alginate-bioglass-encapsulated hAECs	0:42	Sodium alginate-bioglass-encapsulated hAECs	0:42	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	15	34	theme	chemotherapy-induced	2343:2362	arg1	POF					2364:2366	chemotherapy-induced POF	2343:2366	chemotherapy-induced POF	2343:2366	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	3	35	theme	stem	475:478	arg1	cells					480:484	stem cells	475:484	stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel	475:552	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	0	36	theme	ovarian	52:58	arg1	function					60:67	ovarian function	52:67	ovarian function in premature ovarian failure	52:96	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	2	37	from	ability	409:415	arg1	organs					444:449	target organs	437:449	target organs	437:449	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	10	38	theme	cell	1597:1600	arg1	function					1602:1609	granulosa cell function	1587:1609	granulosa cell function	1587:1609	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	11	39	theme	further	1663:1669	arg1	study					1671:1675	The further study	1659:1675	The further study	1659:1675	The further study showed that SA-BG significantly promoted the tube formation of hUVECs in vitro.					
33794993	9	40	theme	components	1410:1419	arg1	effects					1389:1395	the effects	1385:1395	the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs	1385:1485	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	10	41	from	angiogenesis	1633:1644	arg1	mice					1653:1656	POF mice	1649:1656	POF mice	1649:1656	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	15	42	theme	ovarian	2427:2433	arg1	vascularization					2435:2449	ovarian vascularization	2427:2449	ovarian vascularization	2427:2449	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	3	43	theme	cells	480:484	arg1	transplantation					456:470	The transplantation	452:470	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel	452:552	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	3	43	theme	cells	480:484	arg1	strategy					597:604	an effective strategy	584:604	an effective strategy for tissue regeneration	584:628	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	1	44	theme	premature	273:281	arg1	POF					300:302	POF	300:302	POF	300:302	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	44	theme	premature	273:281	arg1	failure					291:297	chemotherapy-induced premature ovarian failure	252:297	chemotherapy-induced premature ovarian failure (POF)	252:303	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	0	45	theme	premature	72:80	arg1	failure					90:96	premature ovarian failure	72:96	premature ovarian failure	72:96	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	12	46	theme	tube	1867:1870	arg1	formation					1840:1848	the formation	1836:1848	the formation of the capillary tube	1836:1870	Moreover, encapsulating hAECs could facilitate the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner.					
33794993	11	47	theme	tube	1722:1725	arg1	formation					1727:1735	the tube formation	1718:1735	the tube formation of hUVECs	1718:1745	The further study showed that SA-BG significantly promoted the tube formation of hUVECs in vitro.					
33794993	2	48	theme	limited	376:382	arg1	number					384:389	limited number	376:389	limited number	376:389	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	15	49	theme	cell	2399:2402	arg1	function					2404:2411	granulosa cell function	2389:2411	granulosa cell function	2389:2411	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	8	50	theme	SA-BG-encapsulated	1185:1202	arg1	hAECs/CM					1204:1211	SA-BG-encapsulated hAECs/CM	1185:1211	SA-BG-encapsulated hAECs/CM	1185:1211	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	6	51	theme	composite	950:958	arg1	hydrogel					960:967	SA-BG composite hydrogel	944:967	SA-BG composite hydrogel	944:967	hAECs or CM were harvested and encapsulated in SA-BG composite hydrogel, which were transplanted onto the injured ovaries of mice with POF.					
33794993	10	52	theme	POF	1649:1651	arg1	mice					1653:1656	POF mice	1649:1656	POF mice	1649:1656	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	13	53	theme	factors	2030:2036	arg1	secretion					2002:2010	the secretion	1998:2010	the secretion of pro-angiogenic factors of hAECs	1998:2045	In addition, we found that SA-BG extracts significantly enhanced the viability of hAECs and stimulated the secretion of pro-angiogenic factors of hAECs.					
33794993	9	54	theme	angiogenic	1459:1468	arg1	factors					1470:1476	angiogenic factors	1459:1476	angiogenic factors	1459:1476	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	3	55	theme	effective	587:595	arg1	transplantation					456:470	The transplantation	452:470	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel	452:552	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	3	55	theme	effective	587:595	arg1	strategy					597:604	an effective strategy	584:604	an effective strategy for tissue regeneration	584:628	The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration.					
33794993	13	56	theme	hAECs	2041:2045	arg1	factors					2030:2036	pro-angiogenic factors	2015:2036	pro-angiogenic factors of hAECs	2015:2045	In addition, we found that SA-BG extracts significantly enhanced the viability of hAECs and stimulated the secretion of pro-angiogenic factors of hAECs.					
33794993	14	57	dep	viability	2177:2185	arg1	the					2173:2175	the	2173:2175	the	2173:2175	Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.					
33794993	2	58	theme	hAECs	343:347	arg1	efficacy					331:338	the therapeutic efficacy	315:338	the therapeutic efficacy of hAECs	315:347	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	14	59	theme	therapeutic	2108:2118	arg1	effects					2120:2126	better therapeutic effects	2101:2126	better therapeutic effects	2101:2126	Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.					
33794993	1	60	theme	strong	203:208	arg1	capability					210:219	a strong capability	201:219	a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF)	201:303	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	8	61	theme	tube	1237:1240	arg1	formation					1242:1250	the tube formation	1233:1250	the tube formation of human umbilical vein epithelial cells (hUVECs)	1233:1300	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	12	62	theme	SA-BG	1818:1822	arg1	effect					1808:1813	the effect	1804:1813	the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner	1804:1892	Moreover, encapsulating hAECs could facilitate the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner.					
33794993	4	63	theme	hAECs	698:702	arg1	potential					685:693	the therapeutic potential	669:693	the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF	669:802	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	6	64	theme	injured	1003:1009	arg1	ovaries					1011:1017	the injured ovaries	999:1017	the injured ovaries of mice with POF	999:1034	hAECs or CM were harvested and encapsulated in SA-BG composite hydrogel, which were transplanted onto the injured ovaries of mice with POF.					
33794993	4	65	link	hAEC-derived	707:718	arg1	medium					732:737	hAEC-derived conditioned medium	707:737	hAEC-derived conditioned medium (CM)	707:742	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	4	65	link	hAEC-derived	707:718	arg1	CM					740:741	CM	740:741	CM	740:741	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	7	66	theme	ovarian	1088:1094	arg1	angiogenesis					1096:1107	ovarian angiogenesis	1088:1107	ovarian angiogenesis	1088:1107	Follicle development, granulosa cell function, and ovarian angiogenesis were evaluated by morphological methods.					
33794993	1	67	theme	ovarian	232:238	arg1	function					240:247	ovarian function	232:247	ovarian function in chemotherapy-induced premature ovarian failure (POF)	232:303	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	8	68	theme	umbilical	1261:1269	arg1	hUVECs					1294:1299	hUVECs	1294:1299	hUVECs	1294:1299	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	8	68	theme	umbilical	1261:1269	arg1	cells					1287:1291	human umbilical vein epithelial cells	1255:1291	human umbilical vein epithelial cells (hUVECs)	1255:1300	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	14	69	theme	hAECs	2213:2217	arg1	viability					2177:2185	viability	2177:2185	viability	2177:2185	Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.					
33794993	14	69	theme	hAECs	2213:2217	arg1	capacity					2201:2208	paracrine capacity	2191:2208	paracrine capacity	2191:2208	Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.					
33794993	4	70	with	mice	769:772	arg1	POF					800:802	chemotherapy-induced POF	779:802	chemotherapy-induced POF	779:802	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	15	71	theme	novel	2474:2478	arg1	strategy					2480:2487	a novel strategy	2472:2487	a novel strategy for the delivery of hAECs for treating POF	2472:2530	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	7	72	theme	granulosa	1059:1067	arg1	function					1074:1081	granulosa cell function	1059:1081	granulosa cell function	1059:1081	Follicle development, granulosa cell function, and ovarian angiogenesis were evaluated by morphological methods.					
33794993	1	73	theme	BACKGROUND	142:151	arg1	hAECs					186:190	hAECs	186:190	hAECs	186:190	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	73	theme	BACKGROUND	142:151	arg1	cells					179:183	BACKGROUND Human amniotic epithelial cells	142:183	BACKGROUND Human amniotic epithelial cells (hAECs)	142:191	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	8	74	theme	epithelial	1276:1285	arg1	hUVECs					1294:1299	hUVECs	1294:1299	hUVECs	1294:1299	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	8	74	theme	epithelial	1276:1285	arg1	cells					1287:1291	human umbilical vein epithelial cells	1255:1291	human umbilical vein epithelial cells (hUVECs)	1255:1300	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	7	75	theme	cell	1069:1072	arg1	function					1074:1081	granulosa cell function	1059:1081	granulosa cell function	1059:1081	Follicle development, granulosa cell function, and ovarian angiogenesis were evaluated by morphological methods.					
33794993	1	76	theme	amniotic	159:166	arg1	hAECs					186:190	hAECs	186:190	hAECs	186:190	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	76	theme	amniotic	159:166	arg1	cells					179:183	BACKGROUND Human amniotic epithelial cells	142:183	BACKGROUND Human amniotic epithelial cells (hAECs)	142:191	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	12	77	theme	encapsulating	1767:1779	arg1	hAECs					1781:1785	encapsulating hAECs	1767:1785	encapsulating hAECs	1767:1785	Moreover, encapsulating hAECs could facilitate the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner.					
33794993	6	78	with	mice	1022:1025	arg1	POF					1032:1034	POF	1032:1034	POF	1032:1034	hAECs or CM were harvested and encapsulated in SA-BG composite hydrogel, which were transplanted onto the injured ovaries of mice with POF.					
33794993	13	79	theme	hAECs	1977:1981	arg1	viability					1964:1972	the viability	1960:1972	the viability of hAECs	1960:1981	In addition, we found that SA-BG extracts significantly enhanced the viability of hAECs and stimulated the secretion of pro-angiogenic factors of hAECs.					
33794993	15	80	theme	SA-BG-encapsulated	2278:2295	arg1	hAECs					2297:2301	SA-BG-encapsulated hAECs	2278:2301	SA-BG-encapsulated hAECs	2278:2301	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	15	81	dep	CONCLUSIONS	2220:2230	arg1	demonstrates					2260:2271	demonstrates	2260:2271	demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF	2260:2530	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	4	82	theme	hAEC-derived	707:718	arg1	medium					732:737	hAEC-derived conditioned medium	707:737	hAEC-derived conditioned medium (CM)	707:742	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	4	82	theme	hAEC-derived	707:718	arg1	CM					740:741	CM	740:741	CM	740:741	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	14	83	theme	paracrine	2191:2199	arg1	capacity					2201:2208	paracrine capacity	2191:2208	paracrine capacity	2191:2208	Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.					
33794993	4	84	theme	medium	732:737	arg1	potential					685:693	the therapeutic potential	669:693	the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF	669:802	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	0	85	theme	factor	124:129	arg1	secretion					131:139	angiogenic factor secretion	113:139	angiogenic factor secretion	113:139	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	9	86	used	used	1356:1359	arg2	ELISA					1345:1349	ELISA	1345:1349	ELISA	1345:1349	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	9	86	used	used	1356:1359	arg2	array					1335:1339	Cytokine array	1326:1339	Cytokine array	1326:1339	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	4	87	theme	chemotherapy-induced	779:798	arg1	POF					800:802	chemotherapy-induced POF	779:802	chemotherapy-induced POF	779:802	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	15	88	theme	hAECs	2509:2513	arg1	delivery					2497:2504	the delivery	2493:2504	the delivery of hAECs	2493:2513	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	0	89	from	function	60:67	arg1	failure					90:96	premature ovarian failure	72:96	premature ovarian failure	72:96	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	2	90	dep	number	384:389	arg1	the					372:374	the	372:374	the	372:374	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	0	91	theme	alginate-bioglass-encapsulated	7:36	arg1	hAECs					38:42	Sodium alginate-bioglass-encapsulated hAECs	0:42	Sodium alginate-bioglass-encapsulated hAECs	0:42	Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion.					
33794993	2	92	theme	proliferative	395:407	arg1	ability					409:415	proliferative ability	395:415	proliferative ability	395:415	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	5	93	theme	chemotherapy	863:874	arg1	drugs					876:880	chemotherapy drugs	863:880	chemotherapy drugs	863:880	METHODS C57BL/6 mice were intraperitoneally injected with chemotherapy drugs to induce POF.					
33794993	4	94	from	SA-BG	760:764	arg1	mice					769:772	mice	769:772	mice with chemotherapy-induced POF	769:802	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	10	95	theme	follicle	1556:1563	arg1	development					1565:1575	follicle development	1556:1575	follicle development	1556:1575	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	2	96	theme	target	437:442	arg1	organs					444:449	target organs	437:449	target organs	437:449	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	5	97	theme	C57BL/6	813:819	arg1	mice					821:824	METHODS C57BL/6 mice	805:824	METHODS C57BL/6 mice	805:824	METHODS C57BL/6 mice were intraperitoneally injected with chemotherapy drugs to induce POF.					
33794993	10	98	theme	hAECs/CM	1538:1545	arg1	transplantation					1500:1514	The transplantation	1496:1514	The transplantation of SA-BG-encapsulated hAECs/CM	1496:1545	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	2	99	theme	hAECs	428:432	arg1	number					384:389	limited number	376:389	limited number	376:389	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	2	99	theme	hAECs	428:432	arg1	ability					409:415	proliferative ability	395:415	proliferative ability	395:415	However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs.					
33794993	10	100	dep	RESULTS	1488:1494	arg1	repaired					1578:1585	repaired	1578:1585	repaired granulosa cell function	1578:1609	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	10	100	dep	RESULTS	1488:1494	arg1	enhanced					1616:1623	enhanced	1616:1623	enhanced ovarian angiogenesis in POF mice	1616:1656	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	10	100	dep	RESULTS	1488:1494	arg1	restored					1547:1554	restored	1547:1554	restored follicle development	1547:1575	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	9	101	theme	bioactive	1400:1408	arg1	components					1410:1419	bioactive components	1400:1419	bioactive components released by SA-BG	1400:1437	Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.					
33794993	8	102	from	effect	1175:1180	arg1	vascularization					1216:1230	vascularization	1216:1230	vascularization	1216:1230	To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro.					
33794993	10	103	theme	granulosa	1587:1595	arg1	function					1602:1609	granulosa cell function	1587:1609	granulosa cell function	1587:1609	RESULTS The transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice.					
33794993	15	104	theme	therapeutic	2321:2331	arg1	effect					2333:2338	a therapeutic effect	2319:2338	a therapeutic effect	2319:2338	CONCLUSIONS The present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.					
33794993	12	105	theme	paracrine	1877:1885	arg1	manner					1887:1892	a paracrine manner	1875:1892	a paracrine manner	1875:1892	Moreover, encapsulating hAECs could facilitate the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner.					
33794993	4	106	theme	current	635:641	arg1	study					643:647	The current study	631:647	The current study	631:647	The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.					
33794993	1	107	theme	chemotherapy-induced	252:271	arg1	POF					300:302	POF	300:302	POF	300:302	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	107	theme	chemotherapy-induced	252:271	arg1	failure					291:297	chemotherapy-induced premature ovarian failure	252:297	chemotherapy-induced premature ovarian failure (POF)	252:303	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	108	theme	ovarian	283:289	arg1	POF					300:302	POF	300:302	POF	300:302	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
33794993	1	108	theme	ovarian	283:289	arg1	failure					291:297	chemotherapy-induced premature ovarian failure	252:297	chemotherapy-induced premature ovarian failure (POF)	252:303	BACKGROUND Human amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF).					
34010762	0	0	theme	wine	79:82	arg1	composition					93:103	wine phenolic composition	79:103	wine phenolic composition	79:103	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	0	1	from	carboxymethylcellulose	14:35	arg1	wine					44:47	red wine	40:47	red wine	40:47	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	3	2	theme	colour	663:668	arg1	intensity					670:678	colour intensity	663:678	colour intensity	663:678	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	2	3	theme	unstable	518:525	arg1	wines					527:531	highly unstable wines	511:531	highly unstable wines	511:531	The degree of substitution of carboxymethylcellulose was important for its efficiency in highly unstable wines.					
34010762	1	4	theme	chromatic	352:360	arg1	composition					339:349	the phenolic composition	326:349	the phenolic composition	326:349	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	4	theme	chromatic	352:360	arg1	characteristics					362:376	chromatic characteristics	352:376	chromatic characteristics	352:376	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	3	5	theme	carboxymethylcellulose	549:570	arg1	Application					534:544	Application	534:544	Application of carboxymethylcellulose	534:570	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	1	6	theme	potassium	264:272	arg1	tartrate					283:290	potassium hydrogen tartrate	264:290	potassium hydrogen tartrate precipitation in red wines	264:317	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	7	7	theme	colouring	1291:1299	arg1	stability					1308:1316	colouring matter stability	1291:1316	colouring matter stability	1291:1316	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	0	8	from	Efficiency	0:9	arg1	wine					44:47	red wine	40:47	red wine	40:47	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	4	9	theme	sensory	824:830	arg1	attributes					832:841	wine sensory attributes	819:841	wine sensory attributes	819:841	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	1	10	theme	hydrogen	274:281	arg1	tartrate					283:290	potassium hydrogen tartrate	264:290	potassium hydrogen tartrate precipitation in red wines	264:317	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	0	11	theme	phenolic	84:91	arg1	composition					93:103	wine phenolic composition	79:103	wine phenolic composition	79:103	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	2	12	from	efficiency	497:506	arg1	wines					527:531	highly unstable wines	511:531	highly unstable wines	511:531	The degree of substitution of carboxymethylcellulose was important for its efficiency in highly unstable wines.					
34010762	4	13	theme	wine	819:822	arg1	attributes					832:841	wine sensory attributes	819:841	wine sensory attributes	819:841	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	3	14	theme	significant	592:602	arg1	change					604:609	a significant change	590:609	a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines	590:722	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	7	15	from	application	1118:1128	arg1	wines					1163:1167	red wines	1159:1167	red wines	1159:1167	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	1	16	theme	tartrate	283:290	arg1	precipitation					292:304	potassium hydrogen tartrate precipitation	264:304	potassium hydrogen tartrate precipitation in red wines	264:317	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	0	17	theme	chromatic	106:114	arg1	characteristics					116:130	chromatic characteristics	106:130	chromatic characteristics	106:130	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	1	18	theme	colouring	382:390	arg1	composition					339:349	the phenolic composition	326:349	the phenolic composition	326:349	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	18	theme	colouring	382:390	arg1	stability					399:407	colouring matter stability	382:407	colouring matter stability	382:407	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	2	19	theme	substitution	436:447	arg1	important					479:487	important	479:487	important	479:487	The degree of substitution of carboxymethylcellulose was important for its efficiency in highly unstable wines.					
34010762	2	19	theme	substitution	436:447	arg1	degree					426:431	The degree	422:431	The degree of substitution of carboxymethylcellulose	422:473	The degree of substitution of carboxymethylcellulose was important for its efficiency in highly unstable wines.					
34010762	0	20	from	wine	44:47	arg1	Efficiency					0:9	Efficiency	0:9	Efficiency of carboxymethylcellulose in red wine	0:47	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	6	21	theme	turbidity	927:935	arg1	use					920:922	The use	916:922	The use of turbidity for evaluating the colouring matter stability of wines	916:990	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	6	22	contain	has	992:994	arg1	use					920:922	The use	916:922	The use of turbidity for evaluating the colouring matter stability of wines	916:990	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	6	22	contain	has	992:994	arg2	drawbacks					1003:1011	severe drawbacks	996:1011	severe drawbacks	996:1011	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	7	23	theme	sensory	1262:1268	arg1	characteristics					1270:1284	sensory characteristics	1262:1284	sensory characteristics	1262:1284	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	6	24	theme	colouring	956:964	arg1	stability					973:981	the colouring matter stability	952:981	the colouring matter stability of wines	952:990	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	1	25	from	precipitation	292:304	arg1	wines					313:317	red wines	309:317	red wines	309:317	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	4	26	from	impact	809:814	arg1	attributes					832:841	wine sensory attributes	819:841	wine sensory attributes	819:841	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	4	27	theme	significant	797:807	arg1	impact					809:814	a significant impact	795:814	a significant impact on wine sensory attributes	795:841	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	4	28	contain	have	790:793	arg1	carboxymethylcellulose					759:780	carboxymethylcellulose	759:780	carboxymethylcellulose	759:780	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	4	28	contain	have	790:793	arg2	impact					809:814	a significant impact	795:814	a significant impact on wine sensory attributes	795:841	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	4	29	theme	Sensory	725:731	arg1	analysis					733:740	Sensory analysis	725:740	Sensory analysis	725:740	Sensory analysis also showed that carboxymethylcellulose doesn't have a significant impact on wine sensory attributes.					
34010762	5	30	theme	colouring	888:896	arg1	stability					905:913	the colouring matter stability	884:913	the colouring matter stability	884:913	Carboxymethylcellulose doesn't decrease the colouring matter stability.					
34010762	7	31	theme	phenolic	1240:1247	arg1	composition					1249:1259	the phenolic composition	1236:1259	the phenolic composition	1236:1259	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	3	32	theme	phenolic	618:625	arg1	composition					650:660	the phenolic, monomeric anthocyanin composition	614:660	the phenolic, monomeric anthocyanin composition	614:660	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	5	33	theme	matter	898:903	arg1	stability					905:913	the colouring matter stability	884:913	the colouring matter stability	884:913	Carboxymethylcellulose doesn't decrease the colouring matter stability.					
34010762	7	34	theme	tartaric	1196:1203	arg1	stability					1205:1213	tartaric stability	1196:1213	tartaric stability	1196:1213	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	3	35	theme	anthocyanin	638:648	arg1	composition					650:660	the phenolic, monomeric anthocyanin composition	614:660	the phenolic, monomeric anthocyanin composition	614:660	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	0	36	theme	carboxymethylcellulose	14:35	arg1	Efficiency					0:9	Efficiency	0:9	Efficiency of carboxymethylcellulose in red wine	0:47	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	1	37	theme	red	309:311	arg1	wines					313:317	red wines	309:317	red wines	309:317	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	7	38	theme	red	1159:1161	arg1	wines					1163:1167	red wines	1159:1167	red wines	1159:1167	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	3	39	dep	phenolic	618:625	arg1	monomeric					628:636	monomeric	628:636	monomeric	628:636	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	6	40	theme	severe	996:1001	arg1	drawbacks					1003:1011	severe drawbacks	996:1011	severe drawbacks	996:1011	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	1	41	theme	carboxymethylcellulose	192:213	arg1	features					226:233	carboxymethylcellulose structural features	192:233	carboxymethylcellulose structural features	192:233	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	6	42	theme	matter	966:971	arg1	stability					973:981	the colouring matter stability	952:981	the colouring matter stability of wines	952:990	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	0	43	theme	matter	146:151	arg1	stability					153:161	colouring matter stability	136:161	colouring matter stability	136:161	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	0	44	dep	stability	58:66	arg1	Effect					69:74	Effect	69:74	stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability	58:161	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	1	45	theme	structural	215:224	arg1	features					226:233	carboxymethylcellulose structural features	192:233	carboxymethylcellulose structural features	192:233	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	0	46	theme	red	40:42	arg1	wine					44:47	red wine	40:47	red wine	40:47	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	0	47	theme	colouring	136:144	arg1	stability					153:161	colouring matter stability	136:161	colouring matter stability	136:161	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	0	48	from	Effect	69:74	arg1	composition					93:103	wine phenolic composition	79:103	wine phenolic composition	79:103	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	0	48	from	Effect	69:74	arg1	characteristics					116:130	chromatic characteristics	106:130	chromatic characteristics	106:130	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	0	48	from	Effect	69:74	arg1	stability					153:161	colouring matter stability	136:161	colouring matter stability	136:161	Efficiency of carboxymethylcellulose in red wine tartaric stability: Effect on wine phenolic composition, chromatic characteristics and colouring matter stability.					
34010762	1	49	theme	features	226:233	arg1	effect					182:187	the effect	178:187	the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability	178:407	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	6	50	theme	turbidity	1020:1028	arg1	related					1058:1064	related	1058:1064	related	1058:1064	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	6	50	theme	turbidity	1020:1028	arg1	value					1030:1034	the turbidity value	1016:1034	the turbidity value measured	1016:1043	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	3	51	theme	chromatic	685:693	arg1	characteristics					695:709	chromatic characteristics	685:709	chromatic characteristics	685:709	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	1	52	theme	matter	392:397	arg1	composition					339:349	the phenolic composition	326:349	the phenolic composition	326:349	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	52	theme	matter	392:397	arg1	stability					399:407	colouring matter stability	382:407	colouring matter stability	382:407	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	7	53	theme	carboxymethylcellulose	1133:1154	arg1	efficient					1172:1180	efficient	1172:1180	efficient	1172:1180	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	7	53	theme	carboxymethylcellulose	1133:1154	arg1	application					1118:1128	the application	1114:1128	the application of carboxymethylcellulose in red wines	1114:1167	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34010762	6	54	theme	wines	986:990	arg1	stability					973:981	the colouring matter stability	952:981	the colouring matter stability of wines	952:990	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	3	55	from	change	604:609	arg1	characteristics					695:709	chromatic characteristics	685:709	chromatic characteristics	685:709	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	3	55	from	change	604:609	arg1	composition					650:660	the phenolic, monomeric anthocyanin composition	614:660	the phenolic, monomeric anthocyanin composition	614:660	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	3	55	from	change	604:609	arg1	intensity					670:678	colour intensity	663:678	colour intensity	663:678	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	1	56	theme	phenolic	330:337	arg1	composition					339:349	the phenolic composition	326:349	the phenolic composition	326:349	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	56	theme	phenolic	330:337	arg1	characteristics					362:376	chromatic characteristics	352:376	chromatic characteristics	352:376	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	56	theme	phenolic	330:337	arg1	stability					399:407	colouring matter stability	382:407	colouring matter stability	382:407	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	3	57	theme	red	714:716	arg1	wines					718:722	red wines	714:722	red wines	714:722	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	6	58	theme	material	1093:1100	arg1	amount					1073:1078	the amount	1069:1078	the amount of suspended material	1069:1100	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	6	58	theme	material	1093:1100	arg1	material					1093:1100	suspended material	1083:1100	suspended material	1083:1100	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	3	59	theme	wines	718:722	arg1	characteristics					695:709	chromatic characteristics	685:709	chromatic characteristics	685:709	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	3	59	theme	wines	718:722	arg1	composition					650:660	the phenolic, monomeric anthocyanin composition	614:660	the phenolic, monomeric anthocyanin composition	614:660	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	3	59	theme	wines	718:722	arg1	intensity					670:678	colour intensity	663:678	colour intensity	663:678	Application of carboxymethylcellulose doesn't result in a significant change in the phenolic, monomeric anthocyanin composition, colour intensity, and chromatic characteristics of red wines.					
34010762	1	60	from	effect	182:187	arg1	efficiency					242:251	the efficiency to prevent potassium hydrogen tartrate precipitation in red wines	238:317	the efficiency to prevent potassium hydrogen tartrate precipitation in red wines	238:317	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	60	from	effect	182:187	arg1	composition					339:349	the phenolic composition	326:349	the phenolic composition	326:349	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	60	from	effect	182:187	arg1	characteristics					362:376	chromatic characteristics	352:376	chromatic characteristics	352:376	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	1	60	from	effect	182:187	arg1	stability					399:407	colouring matter stability	382:407	colouring matter stability	382:407	In this work, the effect of carboxymethylcellulose structural features on the efficiency to prevent potassium hydrogen tartrate precipitation in red wines and on the phenolic composition, chromatic characteristics and colouring matter stability was studied.					
34010762	6	61	theme	suspended	1083:1091	arg1	material					1093:1100	suspended material	1083:1100	suspended material	1083:1100	The use of turbidity for evaluating the colouring matter stability of wines has severe drawbacks as the turbidity value measured might not be related to the amount of suspended material.					
34010762	2	62	theme	carboxymethylcellulose	452:473	arg1	substitution					436:447	substitution	436:447	substitution of carboxymethylcellulose	436:473	The degree of substitution of carboxymethylcellulose was important for its efficiency in highly unstable wines.					
34010762	7	63	theme	matter	1301:1306	arg1	stability					1308:1316	colouring matter stability	1291:1316	colouring matter stability	1291:1316	Therefore, the application of carboxymethylcellulose in red wines is efficient in increasing tartaric stability without impacting on the phenolic composition, sensory characteristics, and colouring matter stability.					
34023688	4	0	from	strengths	492:500	arg1	elongation					444:453	The elongation	440:453	The elongation of the films at breaking and tensile strengths	440:500	The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP.					
34023688	4	1	theme	films	462:466	arg1	elongation					444:453	The elongation	440:453	The elongation of the films at breaking and tensile strengths	440:500	The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP.					
34023688	1	2	theme	composite	157:165	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	2	theme	composite	157:165	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	5	3	located	observed	588:595	arg2	permeability					571:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	5	3	located	observed	588:595	arg1	ratio					615:619	an FG:LMP mass ratio	600:619	an FG:LMP mass ratio of 2:1	600:626	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	1	4	theme	food	238:241	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	4	theme	food	238:241	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	5	with	films	167:171	arg1	ratios					200:205	different FG:LMP mass ratios	178:205	different FG:LMP mass ratios	178:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	5	6	theme	water	558:562	arg1	permeability					571:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	7	7	theme	food	818:821	arg1	simulant					823:830	a food simulant	816:830	a food simulant	816:830	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	1	8	theme	packaging	243:251	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	8	theme	packaging	243:251	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	4	9	theme	tensile	484:490	arg1	strengths					492:500	tensile strengths	484:500	tensile strengths	484:500	The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP.					
34023688	5	10	theme	mass	610:613	arg1	ratio					615:619	an FG:LMP mass ratio	600:619	an FG:LMP mass ratio of 2:1	600:626	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	5	11	theme	vapour	564:569	arg1	permeability					571:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	3	12	theme	blocking	368:375	arg1	efficiency					377:386	the UV (315-400 nm) blocking efficiency	348:386	the UV (315-400 nm) blocking efficiency	348:386	Moreover, the UV (315-400 nm) blocking efficiency and opacity increased with increasing LMP contents.					
34023688	4	13	from	breaking	471:478	arg1	elongation					444:453	The elongation	440:453	The elongation of the films at breaking and tensile strengths	440:500	The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP.					
34023688	2	14	theme	FG	280:281	arg1	contents					283:290	FG contents	280:290	FG contents	280:290	With increasing FG contents, the water solubility of the films decreased.					
34023688	6	15	theme	antioxidant	654:664	arg1	properties					666:675	good antioxidant properties	649:675	good antioxidant properties	649:675	All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation.					
34023688	9	16	theme	complexes	1023:1031	arg1	stability					995:1003	the stability	991:1003	the stability of resveratrol-FG complexes	991:1031	Furthermore, LMP can improve the stability of resveratrol-FG complexes and compete with resveratrol for binding FG to accelerate resveratrol release.					
34023688	1	17	theme	Resveratrol-loaded	94:111	arg1	FG					127:128	FG	127:128	FG	127:128	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	17	theme	Resveratrol-loaded	94:111	arg1	gelatin					118:124	Resveratrol-loaded fish gelatin	94:124	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	6	18	theme	good	649:652	arg1	properties					666:675	good antioxidant properties	649:675	good antioxidant properties	649:675	All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation.					
34023688	1	19	theme	fish	113:116	arg1	FG					127:128	FG	127:128	FG	127:128	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	19	theme	fish	113:116	arg1	gelatin					118:124	Resveratrol-loaded fish gelatin	94:124	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	7	20	theme	%	803:803	arg1	ethanol					805:811	95% ethanol	801:811	95% ethanol as a food simulant	801:830	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	1	21	theme	different	178:186	arg1	ratios					200:205	different FG:LMP mass ratios	178:205	different FG:LMP mass ratios	178:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	4	22	theme	FG	535:536	arg1	ratio					526:530	the ratio	522:530	the ratio of FG and LMP	522:544	The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP.					
34023688	6	23	theme	tallow	740:745	arg1	preservation					747:758	beef tallow preservation	735:758	beef tallow preservation	735:758	All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation.					
34023688	1	24	theme	gelatin	118:124	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	24	theme	gelatin	118:124	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	25	theme	FG	188:189	arg1	ratios					200:205	different FG:LMP mass ratios	178:205	different FG:LMP mass ratios	178:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	7	26	theme	release	765:771	arg1	behaviour					773:781	The release behaviour	761:781	The release behaviour of resveratrol in 95% ethanol as a food simulant	761:830	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	5	27	theme	FG	603:604	arg1	ratio					615:619	an FG:LMP mass ratio	600:619	an FG:LMP mass ratio of 2:1	600:626	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	6	28	theme	beef	735:738	arg1	preservation					747:758	beef tallow preservation	735:758	beef tallow preservation	735:758	All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation.					
34023688	2	29	theme	water	297:301	arg1	solubility					303:312	the water solubility	293:312	the water solubility of the films	293:325	With increasing FG contents, the water solubility of the films decreased.					
34023688	9	30	theme	resveratrol	1091:1101	arg1	release					1103:1109	resveratrol release	1091:1109	resveratrol release	1091:1109	Furthermore, LMP can improve the stability of resveratrol-FG complexes and compete with resveratrol for binding FG to accelerate resveratrol release.					
34023688	5	31	theme	LMP	606:608	arg1	ratio					615:619	an FG:LMP mass ratio	600:619	an FG:LMP mass ratio of 2:1	600:626	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	3	32	theme	UV	352:353	arg1	efficiency					377:386	the UV (315-400 nm) blocking efficiency	348:386	the UV (315-400 nm) blocking efficiency	348:386	Moreover, the UV (315-400 nm) blocking efficiency and opacity increased with increasing LMP contents.					
34023688	5	33	theme	lowest	551:556	arg1	permeability					571:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability	547:582	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	7	34	theme	95	801:802	arg1	%					803:803	%	803:803	%	803:803	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	1	35	theme	LMP	191:193	arg1	ratios					200:205	different FG:LMP mass ratios	178:205	different FG:LMP mass ratios	178:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	7	36	theme	resveratrol	786:796	arg1	behaviour					773:781	The release behaviour	761:781	The release behaviour of resveratrol in 95% ethanol as a food simulant	761:830	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	1	37	theme	mass	195:198	arg1	ratios					200:205	different FG:LMP mass ratios	178:205	different FG:LMP mass ratios	178:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	0	38	theme	pectin-fish	42:52	arg1	films					87:91	pectin-fish gelatin-resveratrol preservative films	42:91	pectin-fish gelatin-resveratrol preservative films	42:91	Fabrication and performance evaluation of pectin-fish gelatin-resveratrol preservative films.					
34023688	2	39	theme	films	321:325	arg1	solubility					303:312	the water solubility	293:312	the water solubility of the films	293:325	With increasing FG contents, the water solubility of the films decreased.					
34023688	8	40	theme	preservation	935:946	arg1	applications					948:959	beef tallow preservation applications	923:959	beef tallow preservation applications	923:959	The fabricated films exhibit significant potential for beef tallow preservation applications.					
34023688	7	41	theme	film	850:853	arg1	composition					855:865	film composition	850:865	film composition	850:865	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	4	42	theme	LMP	542:544	arg1	ratio					526:530	the ratio	522:530	the ratio of FG and LMP	522:544	The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP.					
34023688	8	43	theme	fabricated	872:881	arg1	films					883:887	The fabricated films	868:887	The fabricated films	868:887	The fabricated films exhibit significant potential for beef tallow preservation applications.					
34023688	0	44	theme	preservative	74:85	arg1	films					87:91	pectin-fish gelatin-resveratrol preservative films	42:91	pectin-fish gelatin-resveratrol preservative films	42:91	Fabrication and performance evaluation of pectin-fish gelatin-resveratrol preservative films.					
34023688	8	45	theme	tallow	928:933	arg1	applications					948:959	beef tallow preservation applications	923:959	beef tallow preservation applications	923:959	The fabricated films exhibit significant potential for beef tallow preservation applications.					
34023688	7	46	from	behaviour	773:781	arg1	ethanol					805:811	95% ethanol	801:811	95% ethanol as a food simulant	801:830	The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition.					
34023688	1	47	theme	-low	130:133	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	47	theme	-low	130:133	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	0	48	theme	gelatin-resveratrol	54:72	arg1	films					87:91	pectin-fish gelatin-resveratrol preservative films	42:91	pectin-fish gelatin-resveratrol preservative films	42:91	Fabrication and performance evaluation of pectin-fish gelatin-resveratrol preservative films.					
34023688	5	49	theme	2:1	624:626	arg1	ratio					615:619	an FG:LMP mass ratio	600:619	an FG:LMP mass ratio of 2:1	600:626	The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1.					
34023688	1	50	theme	methoxyl	135:142	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	50	theme	methoxyl	135:142	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	6	51	theme	oil	703:705	arg1	deterioration					707:719	significantly delayed oil deterioration	681:719	significantly delayed oil deterioration	681:719	All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation.					
34023688	1	52	theme	pectin	144:149	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	52	theme	pectin	144:149	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	6	53	theme	delayed	695:701	arg1	deterioration					707:719	significantly delayed oil deterioration	681:719	significantly delayed oil deterioration	681:719	All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation.					
34023688	8	54	theme	significant	897:907	arg1	potential					909:917	significant potential	897:917	significant potential for beef tallow preservation applications	897:959	The fabricated films exhibit significant potential for beef tallow preservation applications.					
34023688	1	55	theme	LMP	152:154	arg1	materials					253:261	food packaging materials	238:261	food packaging materials	238:261	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	1	55	theme	LMP	152:154	arg1	films					167:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films	94:171	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios	94:205	Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials.					
34023688	3	56	theme	LMP	426:428	arg1	contents					430:437	LMP contents	426:437	LMP contents	426:437	Moreover, the UV (315-400 nm) blocking efficiency and opacity increased with increasing LMP contents.					
34023688	0	57	theme	films	87:91	arg1	performance					16:26	performance	16:26	performance	16:26	Fabrication and performance evaluation of pectin-fish gelatin-resveratrol preservative films.					
34023688	0	57	theme	films	87:91	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and performance evaluation of pectin-fish gelatin-resveratrol preservative films.					
34023688	0	58	dep	Fabrication	0:10	arg1	evaluation					28:37	evaluation	28:37	evaluation	28:37	Fabrication and performance evaluation of pectin-fish gelatin-resveratrol preservative films.					
34023688	8	59	theme	beef	923:926	arg1	applications					948:959	beef tallow preservation applications	923:959	beef tallow preservation applications	923:959	The fabricated films exhibit significant potential for beef tallow preservation applications.					
34023688	9	60	theme	resveratrol-FG	1008:1021	arg1	complexes					1023:1031	resveratrol-FG complexes	1008:1031	resveratrol-FG complexes	1008:1031	Furthermore, LMP can improve the stability of resveratrol-FG complexes and compete with resveratrol for binding FG to accelerate resveratrol release.					
34226171	7	0	theme	following	1129:1137	arg1	duration					1191:1198	duration	1191:1198	duration of plateau	1191:1209	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	0	theme	following	1129:1137	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	0	theme	following	1129:1137	arg1	length					1251:1256	length	1251:1256	length of exposure to temperatures over 58 °C	1251:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	0	theme	following	1129:1137	arg1	time					1163:1166	time	1163:1166	time from 58 °C to 62.5 °C	1163:1188	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	0	theme	following	1129:1137	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	2	1	theme	heat	337:340	arg1	air					330:332	air	330:332	air	330:332	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	2	1	theme	heat	337:340	arg1	sources					342:348	heat sources	337:348	heat sources	337:348	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	2	1	theme	heat	337:340	arg1	water					321:325	hot water	317:325	hot water	317:325	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	9	2	theme	Holder	1418:1423	arg1	pasteurization					1425:1438	Holder pasteurization	1418:1438	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.	1406:1714	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	7	3	theme	significant	1098:1108	arg1	differences					1110:1120	significant differences	1098:1120	significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1098:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	0	4	theme	Quality	0:6	arg1	study					8:12	Quality study	0:12	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.	0:100	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	9	5	theme	milk	1449:1452	arg1	pasteurization					1425:1438	Holder pasteurization	1418:1438	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.	1406:1714	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	3	6	theme	infants	540:546	arg1	mothers					529:535	mothers	529:535	mothers of infants delivered at different gestational ages and of different postnatal ages	529:618	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	9	7	theme	pasteurizer	1472:1482	arg1	met					1484:1486	a water-free pasteurizer met	1459:1486	a water-free pasteurizer met	1459:1486	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	6	8	theme	macronutrient	951:963	arg1	composition					965:975	the macronutrient composition	947:975	the macronutrient composition of 8 samples of donor human milk of different volumes	947:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	5	9	theme	Milk	860:863	arg1	Association					870:880	the European Milk Bank Association	847:880	the European Milk Bank Association (EMBA)	847:887	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	5	9	theme	Milk	860:863	arg1	EMBA					883:886	EMBA	883:886	EMBA	883:886	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	3	10	theme	gestational	571:581	arg1	ages					583:586	different gestational ages	561:586	different gestational ages	561:586	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	1	11	theme	Holder	112:117	arg1	pasteurization					119:132	OBJECTIVE Holder pasteurization	102:132	OBJECTIVE Holder pasteurization	102:132	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	1	11	theme	Holder	112:117	arg1	technique					141:149	the technique	137:149	the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents	137:242	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	0	12	from	unit	96:99	arg1	study					8:12	Quality study	0:12	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.	0:100	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	3	13	theme	ages	615:618	arg1	mothers					529:535	mothers	529:535	mothers of infants delivered at different gestational ages and of different postnatal ages	529:618	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	6	14	theme	different	1013:1021	arg1	volumes					1023:1029	different volumes	1013:1029	different volumes	1013:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	0	15	theme	milk	54:57	arg1	pasteurization					24:37	Holder pasteurization	17:37	Holder pasteurization of donor human milk in a neonatal personalized nutrition unit	17:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	3	16	theme	different	595:603	arg1	ages					615:618	different postnatal ages	595:618	different postnatal ages	595:618	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	9	17	theme	European	1529:1536	arg1	Association					1548:1558	the European Milk Bank Association	1525:1558	the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content	1525:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	6	18	theme	milk	1005:1008	arg1	samples					982:988	8 samples	980:988	8 samples of donor human milk of different volumes	980:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	4	19	theme	different	689:697	arg1	phases					699:704	different phases	689:704	different phases of pasteurization	689:722	MATERIAL AND METHODS We analysed the temperatures of different phases of pasteurization with 8 external probes distributed evenly throughout the pasteurizer.					
34226171	9	20	theme	Bank	1543:1546	arg1	Association					1548:1558	the European Milk Bank Association	1525:1558	the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content	1525:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	6	21	theme	donor	993:997	arg1	milk					1005:1008	donor human milk	993:1008	donor human milk of different volumes	993:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	2	22	theme	Different	245:253	arg1	devices					270:276	Different pasteurization devices	245:276	Different pasteurization devices	245:276	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	3	23	from	mothers	529:535	arg1	milk					519:522	donated milk	511:522	donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages	511:618	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	0	24	theme	personalized	73:84	arg1	unit					96:99	a neonatal personalized nutrition unit	62:99	a neonatal personalized nutrition unit	62:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	9	25	from	changes	1646:1652	arg1	content					1665:1671	the fat content	1657:1671	the fat content	1657:1671	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	9	25	from	changes	1646:1652	arg1	content					1707:1713	the protein or lactose content	1684:1713	content	1707:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	6	26	theme	samples	982:988	arg1	composition					965:975	the macronutrient composition	947:975	the macronutrient composition of 8 samples of donor human milk of different volumes	947:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	7	27	dep	parameters	1139:1148	arg1	duration					1191:1198	duration	1191:1198	duration of plateau	1191:1209	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	27	dep	parameters	1139:1148	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	27	dep	parameters	1139:1148	arg1	length					1251:1256	length	1251:1256	length of exposure to temperatures over 58 °C	1251:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	27	dep	parameters	1139:1148	arg1	time					1163:1166	time	1163:1166	time from 58 °C to 62.5 °C	1163:1188	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	27	dep	parameters	1139:1148	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	0	28	from	pasteurization	24:37	arg1	unit					96:99	a neonatal personalized nutrition unit	62:99	a neonatal personalized nutrition unit	62:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	4	29	theme	external	731:738	arg1	probes					740:745	8 external probes	729:745	8 external probes	729:745	MATERIAL AND METHODS We analysed the temperatures of different phases of pasteurization with 8 external probes distributed evenly throughout the pasteurizer.					
34226171	3	30	theme	pasteurization	392:405	arg1	quality					381:387	the quality	377:387	the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	377:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	5	31	theme	range	817:821	arg1	criteria					823:830	the optimal range criteria	805:830	the optimal range criteria established by the European Milk Bank Association (EMBA)	805:887	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	8	32	theme	protein	1378:1384	arg1	content					1397:1403	protein or lactose content	1378:1403	content	1397:1403	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	3	33	theme	water-free	437:446	arg1	pasteurizer					448:458	a new automated water-free pasteurizer	421:458	a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	421:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	8	34	theme	lactose	1389:1395	arg1	content					1397:1403	protein or lactose content	1378:1403	content	1397:1403	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	3	35	theme	new	423:425	arg1	pasteurizer					448:458	a new automated water-free pasteurizer	421:458	a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	421:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	9	36	theme	human	1443:1447	arg1	milk					1449:1452	human milk	1443:1452	human milk	1443:1452	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	0	37	theme	pasteurization	24:37	arg1	study					8:12	Quality study	0:12	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.	0:100	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	7	38	dep	62.5 °C	1182:1188	arg1	to					1179:1180	to	1179:1180	to	1179:1180	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	3	39	theme	neonatal	465:472	arg1	unit					497:500	a neonatal personalized nutrition unit	463:500	a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	463:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	0	40	theme	donor	42:46	arg1	milk					54:57	donor human milk	42:57	donor human milk	42:57	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	2	41	theme	pasteurization	255:268	arg1	devices					270:276	Different pasteurization devices	245:276	Different pasteurization devices	245:276	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	3	42	from	pasteurizer	448:458	arg1	unit					497:500	a neonatal personalized nutrition unit	463:500	a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	463:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	9	43	theme	significant	1634:1644	arg1	changes					1646:1652	significant changes	1634:1652	significant changes in the fat content but not in the protein or lactose content	1634:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	7	44	theme	exposure	1261:1268	arg1	duration					1191:1198	duration	1191:1198	duration of plateau	1191:1209	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	44	theme	exposure	1261:1268	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	44	theme	exposure	1261:1268	arg1	length					1251:1256	length	1251:1256	length of exposure to temperatures over 58 °C	1251:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	44	theme	exposure	1261:1268	arg1	time					1163:1166	time	1163:1166	time from 58 °C to 62.5 °C	1163:1188	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	44	theme	exposure	1261:1268	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	8	45	from	changes	1344:1350	arg1	content					1397:1403	protein or lactose content	1378:1403	content	1397:1403	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	8	45	from	changes	1344:1350	arg1	content					1359:1365	fat content	1355:1365	fat content	1355:1365	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	7	46	dep	RESULTS	1074:1080	arg1	find					1093:1096	find	1093:1096	did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1085:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	47	theme	plateau	1203:1209	arg1	duration					1191:1198	duration	1191:1198	duration of plateau	1191:1209	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	47	theme	plateau	1203:1209	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	47	theme	plateau	1203:1209	arg1	length					1251:1256	length	1251:1256	length of exposure to temperatures over 58 °C	1251:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	47	theme	plateau	1203:1209	arg1	time					1163:1166	time	1163:1166	time from 58 °C to 62.5 °C	1163:1188	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	47	theme	plateau	1203:1209	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	3	48	theme	nutrition	487:495	arg1	unit					497:500	a neonatal personalized nutrition unit	463:500	a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	463:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	1	49	theme	agents	237:242	arg1	transmission					210:221	transmission	210:221	transmission of infectious agents	210:242	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	9	50	theme	fat	1661:1663	arg1	content					1665:1671	the fat content	1657:1671	the fat content	1657:1671	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	8	51	theme	significant	1332:1342	arg1	changes					1344:1350	significant changes	1332:1350	significant changes in fat content but not in protein or lactose content	1332:1403	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	3	52	theme	donated	511:517	arg1	milk					519:522	donated milk	511:522	donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages	511:618	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	5	53	theme	Bank	865:868	arg1	Association					870:880	the European Milk Bank Association	847:880	the European Milk Bank Association (EMBA)	847:887	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	5	53	theme	Bank	865:868	arg1	EMBA					883:886	EMBA	883:886	EMBA	883:886	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	2	54	theme	hot	317:319	arg1	air					330:332	air	330:332	air	330:332	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	2	54	theme	hot	317:319	arg1	sources					342:348	heat sources	337:348	heat sources	337:348	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	2	54	theme	hot	317:319	arg1	water					321:325	hot water	317:325	hot water	317:325	Different pasteurization devices have been described that generally use hot water or air as heat sources.					
34226171	1	55	theme	milk	175:178	arg1	banks					180:184	milk banks	175:184	milk banks	175:184	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	9	56	theme	protein	1688:1694	arg1	content					1707:1713	the protein or lactose content	1684:1713	content	1707:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	9	57	theme	water-free	1461:1470	arg1	met					1484:1486	a water-free pasteurizer met	1459:1486	a water-free pasteurizer met	1459:1486	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	9	58	theme	lactose	1699:1705	arg1	content					1707:1713	the protein or lactose content	1684:1713	content	1707:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	7	59	from	62.5 °C	1182:1188	arg1	duration					1191:1198	duration	1191:1198	duration of plateau	1191:1209	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	59	from	62.5 °C	1182:1188	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	59	from	62.5 °C	1182:1188	arg1	length					1251:1256	length	1251:1256	length of exposure to temperatures over 58 °C	1251:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	59	from	62.5 °C	1182:1188	arg1	time					1163:1166	time	1163:1166	time from 58 °C to 62.5 °C	1163:1188	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	59	from	62.5 °C	1182:1188	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	5	60	theme	European	851:858	arg1	Association					870:880	the European Milk Bank Association	847:880	the European Milk Bank Association (EMBA)	847:887	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	5	60	theme	European	851:858	arg1	EMBA					883:886	EMBA	883:886	EMBA	883:886	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	3	61	theme	different	561:569	arg1	ages					583:586	different gestational ages	561:586	different gestational ages	561:586	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	1	62	theme	OBJECTIVE	102:110	arg1	pasteurization					119:132	OBJECTIVE Holder pasteurization	102:132	OBJECTIVE Holder pasteurization	102:132	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	1	62	theme	OBJECTIVE	102:110	arg1	technique					141:149	the technique	137:149	the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents	137:242	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	4	63	theme	phases	699:704	arg1	temperatures					673:684	the temperatures	669:684	the temperatures of different phases of pasteurization	669:722	MATERIAL AND METHODS We analysed the temperatures of different phases of pasteurization with 8 external probes distributed evenly throughout the pasteurizer.					
34226171	3	64	theme	postnatal	605:613	arg1	ages					615:618	different postnatal ages	595:618	different postnatal ages	595:618	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	5	65	theme	pasteurization	914:927	arg1	quality					903:909	the quality	899:909	the quality of pasteurization	899:927	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	6	66	theme	volumes	1023:1029	arg1	milk					1005:1008	donor human milk	993:1008	donor human milk of different volumes	993:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	9	67	dep	CONCLUSIONS	1406:1416	arg1	pasteurization					1425:1438	Holder pasteurization	1418:1438	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.	1406:1714	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	9	67	dep	CONCLUSIONS	1406:1416	arg1	standards					1500:1508	standards	1500:1508	standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content	1500:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	4	68	theme	pasteurization	709:722	arg1	phases					699:704	different phases	689:704	different phases of pasteurization	689:722	MATERIAL AND METHODS We analysed the temperatures of different phases of pasteurization with 8 external probes distributed evenly throughout the pasteurizer.					
34226171	7	69	theme	highest	1212:1218	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	69	theme	highest	1212:1218	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	8	70	theme	macronutrient	1302:1314	arg1	analysis					1316:1323	The macronutrient analysis	1298:1323	The macronutrient analysis	1298:1323	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	0	71	theme	neonatal	64:71	arg1	unit					96:99	a neonatal personalized nutrition unit	62:99	a neonatal personalized nutrition unit	62:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	9	72	theme	Milk	1538:1541	arg1	Association					1548:1558	the European Milk Bank Association	1525:1558	the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content	1525:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	6	73	theme	human	999:1003	arg1	milk					1005:1008	donor human milk	993:1008	donor human milk of different volumes	993:1029	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	0	74	from	study	8:12	arg1	unit					96:99	a neonatal personalized nutrition unit	62:99	a neonatal personalized nutrition unit	62:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	0	75	theme	nutrition	86:94	arg1	unit					96:99	a neonatal personalized nutrition unit	62:99	a neonatal personalized nutrition unit	62:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	8	76	theme	fat	1355:1357	arg1	content					1359:1365	fat content	1355:1365	fat content	1355:1365	The macronutrient analysis showed significant changes in fat content but not in protein or lactose content.					
34226171	5	77	theme	optimal	809:815	arg1	criteria					823:830	the optimal range criteria	805:830	the optimal range criteria established by the European Milk Bank Association (EMBA)	805:887	We applied the optimal range criteria established by the European Milk Bank Association (EMBA) to assess the quality of pasteurization.					
34226171	0	78	theme	Holder	17:22	arg1	pasteurization					24:37	Holder pasteurization	17:37	Holder pasteurization of donor human milk in a neonatal personalized nutrition unit	17:99	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	9	79	theme	milk	1593:1596	arg1	quantity					1581:1588	the quantity	1577:1588	the quantity of milk	1577:1596	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	6	80	theme	automated	1048:1056	arg1	pasteurization					1058:1071	automated pasteurization	1048:1071	automated pasteurization	1048:1071	We also analysed the macronutrient composition of 8 samples of donor human milk of different volumes before and after automated pasteurization.					
34226171	4	81	dep	MATERIAL	636:643	arg1	analysed					660:667	analysed	660:667	analysed the temperatures of different phases of pasteurization with 8 external probes	660:745	MATERIAL AND METHODS We analysed the temperatures of different phases of pasteurization with 8 external probes distributed evenly throughout the pasteurizer.					
34226171	7	82	from	differences	1110:1120	arg1	duration					1191:1198	duration	1191:1198	duration of plateau	1191:1209	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	82	from	differences	1110:1120	arg1	parameters					1139:1148	the following parameters	1125:1148	the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C	1125:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	82	from	differences	1110:1120	arg1	length					1251:1256	length	1251:1256	length of exposure to temperatures over 58 °C	1251:1295	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	82	from	differences	1110:1120	arg1	time					1163:1166	time	1163:1166	time from 58 °C to 62.5 °C	1163:1188	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	7	82	from	differences	1110:1120	arg1	temperature					1220:1230	highest temperature	1212:1230	highest temperature during plateau	1212:1245	RESULTS We did not find significant differences in the following parameters under study: time from 58 °C to 62.5 °C, duration of plateau, highest temperature during plateau and length of exposure to temperatures over 58 °C.					
34226171	9	83	with	pasteurization	1425:1438	arg1	met					1484:1486	a water-free pasteurizer met	1459:1486	a water-free pasteurizer met	1459:1486	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	3	84	theme	automated	427:435	arg1	pasteurizer					448:458	a new automated water-free pasteurizer	421:458	a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	421:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	0	85	theme	human	48:52	arg1	milk					54:57	donor human milk	42:57	donor human milk	42:57	Quality study of Holder pasteurization of donor human milk in a neonatal personalized nutrition unit.					
34226171	3	86	theme	personalized	474:485	arg1	unit					497:500	a neonatal personalized nutrition unit	463:500	a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized	463:633	In our study, we analysed the quality of pasteurization achieved with a new automated water-free pasteurizer in a neonatal personalized nutrition unit in which donated milk from mothers of infants delivered at different gestational ages and of different postnatal ages is pasteurized.					
34226171	9	87	dep	standards	1500:1508	arg1	recommended					1510:1520	recommended	1510:1520	standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content	1500:1713	CONCLUSIONS Holder pasteurization of human milk with a water-free pasteurizer met the quality standards recommended by the European Milk Bank Association independently of the quantity of milk pasteurized in each bottle and with significant changes in the fat content but not in the protein or lactose content.					
34226171	1	88	theme	transmission	210:221	arg1	risk					202:205	the risk	198:205	the risk of transmission of infectious agents	198:242	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34226171	1	89	theme	infectious	226:235	arg1	agents					237:242	infectious agents	226:242	infectious agents	226:242	OBJECTIVE Holder pasteurization is the technique used most frequently in milk banks to minimize the risk of transmission of infectious agents.					
34862928	8	0	theme	MET-loaded	1128:1137	arg1	scaffolds					1156:1164	the MET-loaded β-TCP/CTS/SBA-15 scaffolds	1124:1164	the MET-loaded β-TCP/CTS/SBA-15 scaffolds	1124:1164	Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis.					
34862928	4	1	theme	CTS	662:664	arg1	properties					724:733	the anti-inflammatory properties	702:733	the anti-inflammatory properties of MET	702:740	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	1	theme	CTS	662:664	arg1	biocompatibility					642:657	the excellent biocompatibility	628:657	the excellent biocompatibility of CTS	628:664	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	1	theme	CTS	662:664	arg1	bioactivity					676:686	the good bioactivity	667:686	the good bioactivity of β-TCP	667:695	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	7	2	theme	histological	1006:1017	arg1	analysis					1019:1026	histological analysis	1006:1026	histological analysis	1006:1026	After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization.					
34862928	0	3	theme	rat	94:96	arg1	model					98:102	a rat model	92:102	a rat model of periodontitis	92:119	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	6	4	theme	composite	882:890	arg1	scaffold					892:899	This MET composite scaffold	873:899	This MET composite scaffold	873:899	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	10	5	theme	bone	1471:1474	arg1	defects					1476:1482	alveolar bone defects	1462:1482	alveolar bone defects	1462:1482	Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.					
34862928	2	6	theme	periodontitis	358:370	arg1	defect					348:353	the alveolar bone defect	330:353	the alveolar bone defect of periodontitis	330:370	It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis.					
34862928	0	7	theme	periodontitis	107:119	arg1	model					98:102	a rat model	92:102	a rat model of periodontitis	92:119	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	6	8	theme	MET	878:880	arg1	scaffold					892:899	This MET composite scaffold	873:899	This MET composite scaffold	873:899	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	10	9	theme	promising	1429:1437	arg1	strategy					1439:1446	a new and promising strategy	1419:1446	a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis	1419:1519	Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.					
34862928	2	10	theme	therapeutic	299:309	arg1	scaffold					311:318	a therapeutic scaffold	297:318	a therapeutic scaffold to repair the alveolar bone defect of periodontitis	297:370	It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis.					
34862928	9	11	theme	MET-loaded	1283:1292	arg1	scaffolds					1311:1319	MET-loaded β-TCP/CTS/SBA-15 scaffolds	1283:1319	MET-loaded β-TCP/CTS/SBA-15 scaffolds	1283:1319	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	8	12	theme	β-TCP/CTS/SBA-15	1139:1154	arg1	scaffolds					1156:1164	the MET-loaded β-TCP/CTS/SBA-15 scaffolds	1124:1164	the MET-loaded β-TCP/CTS/SBA-15 scaffolds	1124:1164	Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis.					
34862928	9	13	theme	β-TCP/CTS/SBA-15	1294:1309	arg1	scaffolds					1311:1319	MET-loaded β-TCP/CTS/SBA-15 scaffolds	1283:1319	MET-loaded β-TCP/CTS/SBA-15 scaffolds	1283:1319	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	6	14	theme	rats	952:955	arg1	area					944:947	the alveolar bone defects area	918:947	the alveolar bone defects area of rats with periodontitis	918:974	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	2	15	theme	alveolar	334:341	arg1	defect					348:353	the alveolar bone defect	330:353	the alveolar bone defect of periodontitis	330:370	It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis.					
34862928	8	16	theme	alveolar	1175:1182	arg1	regeneration					1189:1200	alveolar bone regeneration	1175:1200	alveolar bone regeneration	1175:1200	Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis.					
34862928	5	17	theme	β-TCP/CTS/SBA-15	758:773	arg1	scaffolds					775:783	The MET-loaded β-TCP/CTS/SBA-15 scaffolds	743:783	The MET-loaded β-TCP/CTS/SBA-15 scaffolds	743:783	The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro.					
34862928	1	18	theme	alveolar	201:208	arg1	resorption					215:224	alveolar bone resorption	201:224	alveolar bone resorption	201:224	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	0	19	theme	bone	71:74	arg1	regeneration					76:87	alveolar bone regeneration	62:87	alveolar bone regeneration	62:87	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	3	20	theme	composite	406:414	arg1	MET					444:446	MET	444:446	MET	444:446	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	20	theme	composite	406:414	arg1	metformin					433:441	a new composite scaffold loading metformin	400:441	a new composite scaffold loading metformin (MET)	400:447	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	1	21	theme	attachment	250:259	arg1	loss					230:233	loss	230:233	loss of periodontal attachment	230:259	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	1	21	theme	attachment	250:259	arg1	resorption					215:224	alveolar bone resorption	201:224	alveolar bone resorption	201:224	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	0	22	theme	β-TCP/CTS/SBA-15	17:32	arg1	scaffolds					44:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds	0:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds	0:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	8	23	theme	bone	1184:1187	arg1	regeneration					1189:1200	alveolar bone regeneration	1175:1200	alveolar bone regeneration	1175:1200	Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis.					
34862928	7	24	theme	different	1055:1063	arg1	degrees					1065:1071	different degrees	1055:1071	different degrees of healing and mineralization	1055:1101	After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization.					
34862928	10	25	theme	new	1421:1423	arg1	strategy					1439:1446	a new and promising strategy	1419:1446	a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis	1419:1519	Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.					
34862928	4	26	theme	β-TCP	691:695	arg1	properties					724:733	the anti-inflammatory properties	702:733	the anti-inflammatory properties of MET	702:740	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	26	theme	β-TCP	691:695	arg1	biocompatibility					642:657	the excellent biocompatibility	628:657	the excellent biocompatibility of CTS	628:664	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	26	theme	β-TCP	691:695	arg1	bioactivity					676:686	the good bioactivity	667:686	the good bioactivity of β-TCP	667:695	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	3	27	theme	loading	425:431	arg1	MET					444:446	MET	444:446	MET	444:446	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	27	theme	loading	425:431	arg1	metformin					433:441	a new composite scaffold loading metformin	400:441	a new composite scaffold loading metformin (MET)	400:447	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	0	28	theme	Metformin-loaded	0:15	arg1	scaffolds					44:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds	0:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds	0:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	9	29	theme	positive	1328:1335	arg1	effect					1337:1342	a positive effect	1326:1342	a positive effect on alveolar bone regeneration	1326:1372	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	4	30	theme	good	671:674	arg1	bioactivity					676:686	the good bioactivity	667:686	the good bioactivity of β-TCP	667:695	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	31	theme	anti-inflammatory	706:722	arg1	properties					724:733	the anti-inflammatory properties	702:733	the anti-inflammatory properties of MET	702:740	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	1	32	theme	bone	210:213	arg1	resorption					215:224	alveolar bone resorption	201:224	alveolar bone resorption	201:224	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	10	33	theme	periodontitis	1507:1519	arg1	condition					1494:1502	the condition	1490:1502	the condition of periodontitis	1490:1519	Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.					
34862928	9	34	contain	have	1321:1324	arg1	scaffolds					1311:1319	MET-loaded β-TCP/CTS/SBA-15 scaffolds	1283:1319	MET-loaded β-TCP/CTS/SBA-15 scaffolds	1283:1319	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	9	34	contain	have	1321:1324	arg2	effect					1337:1342	a positive effect	1326:1342	a positive effect on alveolar bone regeneration	1326:1372	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	5	35	theme	cell	801:804	arg1	adhesion					806:813	improved cell adhesion	792:813	improved cell adhesion	792:813	The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro.					
34862928	0	36	theme	composite	34:42	arg1	scaffolds					44:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds	0:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds	0:52	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	6	37	theme	defects	936:942	arg1	area					944:947	the alveolar bone defects area	918:947	the alveolar bone defects area of rats with periodontitis	918:974	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	0	38	theme	alveolar	62:69	arg1	regeneration					76:87	alveolar bone regeneration	62:87	alveolar bone regeneration	62:87	Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis.					
34862928	6	39	theme	bone	931:934	arg1	area					944:947	the alveolar bone defects area	918:947	the alveolar bone defects area of rats with periodontitis	918:974	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	3	40	theme	scaffold	416:423	arg1	MET					444:446	MET	444:446	MET	444:446	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	40	theme	scaffold	416:423	arg1	metformin					433:441	a new composite scaffold loading metformin	400:441	a new composite scaffold loading metformin (MET)	400:447	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	6	41	theme	alveolar	922:929	arg1	area					944:947	the alveolar bone defects area	918:947	the alveolar bone defects area of rats with periodontitis	918:974	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	5	42	theme	good	841:844	arg1	biocompatibility					846:861	good biocompatibility	841:861	good biocompatibility	841:861	The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro.					
34862928	3	43	theme	mesoporous	561:570	arg1	SBA-15					580:585	SBA-15	580:585	SBA-15	580:585	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	43	theme	mesoporous	561:570	arg1	silica					572:577	the mesoporous silica	557:577	the mesoporous silica (SBA-15)	557:586	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	5	44	theme	appropriate	816:826	arg1	porosity					828:835	appropriate porosity	816:835	appropriate porosity	816:835	The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro.					
34862928	8	45	theme	periodontitis	1220:1232	arg1	model					1211:1215	a rat model	1205:1215	a rat model of periodontitis	1205:1232	Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis.					
34862928	9	46	theme	bone	1356:1359	arg1	regeneration					1361:1372	alveolar bone regeneration	1347:1372	alveolar bone regeneration	1347:1372	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	5	47	theme	MET-loaded	747:756	arg1	scaffolds					775:783	The MET-loaded β-TCP/CTS/SBA-15 scaffolds	743:783	The MET-loaded β-TCP/CTS/SBA-15 scaffolds	743:783	The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro.					
34862928	3	48	theme	new	402:404	arg1	MET					444:446	MET	444:446	MET	444:446	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	48	theme	new	402:404	arg1	metformin					433:441	a new composite scaffold loading metformin	400:441	a new composite scaffold loading metformin (MET)	400:447	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	49	theme	freeze-drying	462:474	arg1	method					476:481	the freeze-drying method	458:481	the freeze-drying method	458:481	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	7	50	theme	healing	1076:1082	arg1	degrees					1065:1071	different degrees	1055:1071	different degrees of healing and mineralization	1055:1101	After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization.					
34862928	8	51	theme	rat	1207:1209	arg1	model					1211:1215	a rat model	1205:1215	a rat model of periodontitis	1205:1232	Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis.					
34862928	9	52	theme	alveolar	1347:1354	arg1	regeneration					1361:1372	alveolar bone regeneration	1347:1372	alveolar bone regeneration	1347:1372	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	4	53	theme	excellent	632:640	arg1	biocompatibility					642:657	the excellent biocompatibility	628:657	the excellent biocompatibility of CTS	628:664	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	1	54	theme	progressive	141:151	arg1	disease					177:183	a progressive infectious inflammatory disease	139:183	a progressive infectious inflammatory disease	139:183	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	1	54	theme	progressive	141:151	arg1	Periodontitis					122:134	Periodontitis	122:134	Periodontitis	122:134	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	1	55	theme	periodontal	238:248	arg1	attachment					250:259	periodontal attachment	238:259	periodontal attachment	238:259	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	4	56	theme	MET	738:740	arg1	properties					724:733	the anti-inflammatory properties	702:733	the anti-inflammatory properties of MET	702:740	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	56	theme	MET	738:740	arg1	biocompatibility					642:657	the excellent biocompatibility	628:657	the excellent biocompatibility of CTS	628:664	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	4	56	theme	MET	738:740	arg1	bioactivity					676:686	the good bioactivity	667:686	the good bioactivity of β-TCP	667:695	The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET.					
34862928	1	57	theme	infectious	153:162	arg1	disease					177:183	a progressive infectious inflammatory disease	139:183	a progressive infectious inflammatory disease	139:183	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	1	57	theme	infectious	153:162	arg1	Periodontitis					122:134	Periodontitis	122:134	Periodontitis	122:134	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	2	58	theme	bone	343:346	arg1	defect					348:353	the alveolar bone defect	330:353	the alveolar bone defect of periodontitis	330:370	It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis.					
34862928	10	59	theme	alveolar	1462:1469	arg1	defects					1476:1482	alveolar bone defects	1462:1482	alveolar bone defects	1462:1482	Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.					
34862928	9	60	from	effect	1337:1342	arg1	regeneration					1361:1372	alveolar bone regeneration	1347:1372	alveolar bone regeneration	1347:1372	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	1	61	theme	inflammatory	164:175	arg1	disease					177:183	a progressive infectious inflammatory disease	139:183	a progressive infectious inflammatory disease	139:183	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	1	61	theme	inflammatory	164:175	arg1	Periodontitis					122:134	Periodontitis	122:134	Periodontitis	122:134	Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment.					
34862928	6	62	with	rats	952:955	arg1	periodontitis					962:974	periodontitis	962:974	periodontitis	962:974	This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis.					
34862928	9	63	theme	first	1265:1269	arg1	this					1253:1256	this	1253:1256	this	1253:1256	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	9	63	theme	first	1265:1269	arg1	report					1271:1276	the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis	1261:1389	the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis	1261:1389	To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis.					
34862928	5	64	theme	improved	792:799	arg1	adhesion					806:813	improved cell adhesion	792:813	improved cell adhesion	792:813	The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro.					
34862928	3	65	theme	β-tricalcium	506:517	arg1	β-TCP					530:534	β-TCP	530:534	β-TCP	530:534	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	3	65	theme	β-tricalcium	506:517	arg1	phosphate					519:527	β-tricalcium phosphate	506:527	β-tricalcium phosphate (β-TCP)	506:535	In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15).					
34862928	7	66	theme	mineralization	1088:1101	arg1	degrees					1065:1071	different degrees	1055:1071	different degrees of healing and mineralization	1055:1101	After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization.					
32846620	3	0	theme	macaúba	798:804	arg1	MC					819:820	MC	819:820	MC	819:820	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	3	0	theme	macaúba	798:804	arg1	cake					813:816	macaúba kernel cake	798:816	macaúba kernel cake (MC)	798:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	4	1	theme	fruits	838:843	arg1	characterization					845:860	the fruits characterization	834:860	the fruits characterization	834:860	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	6	2	theme	relevant	1559:1566	arg1	reduction					1571:1579	a more relevant pH reduction	1552:1579	a more relevant pH reduction when compared to the commercial prebiotic FOS	1552:1625	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	9	3	theme	potential	2008:2016	arg1	components					2041:2050	their prebiotic potential, and maybe interesting components	1992:2050	their prebiotic potential, and maybe interesting components	1992:2050	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	5	4	contain	has	1434:1436	arg1	JP					1301:1302	JP	1301:1302	JP	1301:1302	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	4	contain	has	1434:1436	arg2	content					1460:1466	a high total phenolic content	1438:1466	a high total phenolic content (850.62 mg GAE·100 g-1)	1438:1490	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	4	contain	has	1434:1436	arg2	GAE·100 g-1					1479:1489	850.62 mg GAE·100 g-1	1469:1489	850.62 mg GAE·100 g-1	1469:1489	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	2	5	theme	kernel	521:526	arg1	byproduct					556:564	a nutritionally rich byproduct	535:564	a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential	535:632	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	2	5	theme	kernel	521:526	arg1	cakes					528:532	the kernel cakes	517:532	the kernel cakes	517:532	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	8	6	theme	acetic	1841:1846	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	4	7	theme	antioxidant	941:951	arg1	activity					953:960	antioxidant activity	941:960	antioxidant activity	941:960	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	5	8	theme	fruit·g-1	1403:1411	arg1	DPPH					1413:1416	2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH	1364:1416	DPPH	1413:1416	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	9	theme	JC	1230:1231	arg1	%					1228:1228	37.87% JC	1223:1231	37.87% JC	1223:1231	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	6	10	theme	prebiotic	1613:1621	arg1	FOS					1623:1625	the commercial prebiotic FOS	1598:1625	the commercial prebiotic FOS	1598:1625	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	9	11	theme	nutritional	2068:2078	arg1	value					2080:2084	the nutritional value	2064:2084	the nutritional value of foods	2064:2093	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	2	12	theme	oil	452:454	arg1	extraction					456:465	oil extraction	452:465	oil extraction	452:465	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	4	13	theme	compounds	918:926	arg1	content					928:934	phenolic compounds content	909:934	phenolic compounds content	909:934	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	5	14	theme	µMTrolox·g-1	1372:1383	arg1	fruit					1385:1389	2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH	1364:1416	fruit	1385:1389	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	1	15	theme	production	326:335	arg1	cost					337:340	a low production cost	320:340	a low production cost	320:340	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	0	16	from	Jerivá	49:54	arg1	kernel					32:37	kernel	32:37	kernel	32:37	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	16	from	Jerivá	49:54	arg1	pulp					23:26	pulp	23:26	pulp	23:26	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	16	from	Jerivá	49:54	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).	0:124	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	8	17	theme	health	1875:1880	arg1	benefits					1882:1889	health benefits	1875:1889	health benefits	1875:1889	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	4	18	theme	antimicrobial	986:998	arg1	capacity					1017:1024	the antimicrobial and fermentative capacity	982:1024	capacity	1017:1024	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	3	19	theme	jerivá	733:738	arg1	JP					746:747	JP	746:747	JP	746:747	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	3	19	theme	jerivá	733:738	arg1	pulp					740:743	jerivá pulp	733:743	jerivá pulp (JP)	733:748	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	0	20	from	Macaúba	84:90	arg1	kernel					32:37	kernel	32:37	kernel	32:37	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	20	from	Macaúba	84:90	arg1	pulp					23:26	pulp	23:26	pulp	23:26	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	20	from	Macaúba	84:90	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).	0:124	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	4	21	theme	fermentative	1004:1015	arg1	capacity					1017:1024	the antimicrobial and fermentative capacity	982:1024	capacity	1017:1024	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	2	22	dep	added	578:582	arg1	have					609:612	have	609:612	may have prebiotic potential	605:632	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	3	23	theme	work	668:671	arg1	objective					650:658	the objective	646:658	the objective of this work	646:671	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	1	24	theme	subtropical	273:283	arg1	areas					285:289	tropical and subtropical areas	260:289	tropical and subtropical areas of Latin America	260:306	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	2	25	theme	lipids	412:417	arg1	content					401:407	the high content	392:407	the high content of lipids	392:417	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	5	26	theme	antioxidant	1268:1278	arg1	activity					1280:1287	high antioxidant activity	1263:1287	high antioxidant activity	1263:1287	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	2	27	theme	rich	551:554	arg1	byproduct					556:564	a nutritionally rich byproduct	535:564	a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential	535:632	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	2	27	theme	rich	551:554	arg1	cakes					528:532	the kernel cakes	517:532	the kernel cakes	517:532	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	1	28	theme	America	300:306	arg1	areas					285:289	tropical and subtropical areas	260:289	tropical and subtropical areas of Latin America	260:306	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	3	29	theme	macaúba	751:757	arg1	MP					765:766	MP	765:766	MP	765:766	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	3	29	theme	macaúba	751:757	arg1	pulp					759:762	macaúba pulp	751:762	macaúba pulp (MP)	751:767	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	5	30	theme	total	1445:1449	arg1	content					1460:1466	a high total phenolic content	1438:1466	a high total phenolic content (850.62 mg GAE·100 g-1)	1438:1490	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	30	theme	total	1445:1449	arg1	GAE·100 g-1					1479:1489	850.62 mg GAE·100 g-1	1469:1489	850.62 mg GAE·100 g-1	1469:1489	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	0	31	theme	Prebiotic	0:8	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).	0:124	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	1	32	theme	Arecaceae	220:228	arg1	family					230:235	the Arecaceae family	216:235	the Arecaceae family	216:235	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	3	33	theme	jerivá	770:775	arg1	JC					790:791	JC	790:791	JC	790:791	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	3	33	theme	jerivá	770:775	arg1	cake					784:787	jerivá kernel cake	770:787	jerivá kernel cake (JC)	770:792	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	6	34	theme	probiotic	1530:1538	arg1	strains					1540:1546	probiotic strains	1530:1546	probiotic strains	1530:1546	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	4	35	theme	lactis	1045:1050	arg1	capacity					1017:1024	the antimicrobial and fermentative capacity	982:1024	capacity	1017:1024	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	0	36	dep	Jerivá	49:54	arg1	fruits					97:102	palm fruits	92:102	palm fruits (Acrocomia aculeata)	92:123	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	36	dep	Jerivá	49:54	arg1	aculeata					115:122	aculeata	115:122	aculeata	115:122	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	5	37	theme	kernel	1159:1164	arg1	cakes					1166:1170	kernel cakes	1159:1170	kernel cakes	1159:1170	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	9	38	theme	oil	1942:1944	arg1	extraction					1946:1955	macaúba oil extraction	1934:1955	macaúba oil extraction	1934:1955	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	2	39	used	used	443:446	arg2	fruits					426:431	their fruits	420:431	their fruits	420:431	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	8	40	theme	use	1741:1743	arg1	JP					1745:1746	use JP	1741:1746	use JP	1741:1746	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	1	41	theme	tropical	260:267	arg1	areas					285:289	tropical and subtropical areas	260:289	tropical and subtropical areas of Latin America	260:306	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	3	42	theme	cake	784:787	arg1	potential					720:728	the prebiotic potential	706:728	the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC)	706:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	1	43	theme	Acrocomia	178:186	arg1	macaúba					169:175	the macaúba	165:175	the macaúba (Acrocomia aculeata)	165:196	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	43	theme	Acrocomia	178:186	arg1	aculeata					188:195	Acrocomia aculeata	178:195	Acrocomia aculeata	178:195	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	3	44	theme	kernel	806:811	arg1	MC					819:820	MC	819:820	MC	819:820	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	3	44	theme	kernel	806:811	arg1	cake					813:816	macaúba kernel cake	798:816	macaúba kernel cake (MC)	798:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	8	45	theme	fatty	1789:1793	arg1	acids					1795:1799	short-chain fatty acids	1777:1799	short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits	1777:1889	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	8	45	theme	fatty	1789:1793	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	5	46	theme	dietary	1197:1203	arg1	fiber					1205:1209	dietary fiber	1197:1209	dietary fiber	1197:1209	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	1	47	contain	have	315:318	arg1	trees					207:211	palm trees	202:211	palm trees	202:211	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	47	contain	have	315:318	arg2	productivity					351:362	high productivity	346:362	high productivity	346:362	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	47	contain	have	315:318	arg2	cost					337:340	a low production cost	320:340	a low production cost	320:340	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	47	contain	have	315:318	arg1	macaúba					169:175	the macaúba	165:175	the macaúba (Acrocomia aculeata)	165:196	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	47	contain	have	315:318	arg1	jerivá					130:135	The jerivá	126:135	The jerivá (Syagrus romanzoffiana)	126:159	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	6	48	theme	pH	1568:1569	arg1	reduction					1571:1579	a more relevant pH reduction	1552:1579	a more relevant pH reduction when compared to the commercial prebiotic FOS	1552:1625	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	5	49	theme	850.62 mg	1469:1477	arg1	content					1460:1466	a high total phenolic content	1438:1466	a high total phenolic content (850.62 mg GAE·100 g-1)	1438:1490	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	49	theme	850.62 mg	1469:1477	arg1	GAE·100 g-1					1479:1489	850.62 mg GAE·100 g-1	1469:1489	850.62 mg GAE·100 g-1	1469:1489	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	1	50	theme	palm	202:205	arg1	trees					207:211	palm trees	202:211	palm trees	202:211	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	50	theme	palm	202:205	arg1	macaúba					169:175	the macaúba	165:175	the macaúba (Acrocomia aculeata)	165:196	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	50	theme	palm	202:205	arg1	jerivá					130:135	The jerivá	126:135	The jerivá (Syagrus romanzoffiana)	126:159	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	8	51	theme	lactic	1809:1814	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	5	52	theme	high	1327:1330	arg1	values					1332:1337	the high values	1323:1337	the high values found for ABTS	1323:1352	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	8	53	theme	propionic	1817:1825	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	9	54	theme	interesting	2029:2039	arg1	components					2041:2050	their prebiotic potential, and maybe interesting components	1992:2050	their prebiotic potential, and maybe interesting components	1992:2050	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	8	55	theme	butyric	1828:1834	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	5	56	theme	96.97 g	1395:1401	arg1	DPPH					1413:1416	2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH	1364:1416	DPPH	1413:1416	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	7	57	theme	strains	1694:1700	arg1	growth					1680:1685	the growth	1676:1685	the growth of the strains	1676:1700	However, MP, JC, and MC were also able to favor the growth of the strains.					
32846620	4	58	theme	phenolic	909:916	arg1	content					928:934	phenolic compounds content	909:934	phenolic compounds content	909:934	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	6	59	theme	commercial	1602:1611	arg1	FOS					1623:1625	the commercial prebiotic FOS	1598:1625	the commercial prebiotic FOS	1598:1625	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	5	60	theme	2498.49	1364:1370	arg1	fruit					1385:1389	2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH	1364:1416	fruit	1385:1389	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	1	61	theme	low	322:324	arg1	cost					337:340	a low production cost	320:340	a low production cost	320:340	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	9	62	contain	have	1957:1960	arg1	byproducts					1907:1916	the byproducts	1903:1916	the byproducts from jerivá and macaúba oil extraction	1903:1955	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	9	62	contain	have	1957:1960	arg2	characteristics					1962:1976	characteristics	1962:1976	characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods	1962:2093	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	8	63	theme	capable	1854:1860	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	9	64	theme	foods	2089:2093	arg1	value					2080:2084	the nutritional value	2064:2084	the nutritional value of foods	2064:2093	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	9	65	from	extraction	1946:1955	arg1	byproducts					1907:1916	the byproducts	1903:1916	the byproducts from jerivá and macaúba oil extraction	1903:1955	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	5	66	dep	%	1253:1253	arg1	MC					1255:1256	MC	1255:1256	35.81% MC	1248:1256	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	2	67	theme	human	589:593	arg1	food					595:598	human food	589:598	human food	589:598	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	1	68	theme	high	346:349	arg1	productivity					351:362	high productivity	346:362	high productivity	346:362	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	3	69	theme	pulp	740:743	arg1	potential					720:728	the prebiotic potential	706:728	the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC)	706:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	2	70	theme	prebiotic	614:622	arg1	potential					624:632	prebiotic potential	614:632	prebiotic potential	614:632	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	5	71	theme	Jerivá	1130:1135	arg1	pulps					1149:1153	Jerivá and macaúba pulps	1130:1153	Jerivá and macaúba pulps	1130:1153	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	9	72	from	jerivá	1923:1928	arg1	byproducts					1907:1916	the byproducts	1903:1916	the byproducts from jerivá and macaúba oil extraction	1903:1955	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	5	73	dep	%	1239:1239	arg1	MP					1241:1242	MP	1241:1242	19.95% MP	1234:1242	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	0	74	from	potential	10:18	arg1	Jerivá					49:54	Jerivá	49:54	Jerivá	49:54	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	74	from	potential	10:18	arg1	romanzoffiana					65:77	romanzoffiana	65:77	romanzoffiana	65:77	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	74	from	potential	10:18	arg1	Macaúba					84:90	Macaúba	84:90	Macaúba	84:90	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	5	75	theme	high	1263:1266	arg1	activity					1280:1287	high antioxidant activity	1263:1287	high antioxidant activity	1263:1287	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	4	76	theme	casei	1067:1071	arg1	capacity					1017:1024	the antimicrobial and fermentative capacity	982:1024	capacity	1017:1024	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	3	77	theme	prebiotic	710:718	arg1	potential					720:728	the prebiotic potential	706:728	the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC)	706:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	0	78	theme	palm	92:95	arg1	fruits					97:102	palm fruits	92:102	palm fruits (Acrocomia aculeata)	92:123	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	0	78	theme	palm	92:95	arg1	aculeata					115:122	aculeata	115:122	aculeata	115:122	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	5	79	theme	high	1182:1185	arg1	levels					1187:1192	high levels	1182:1192	high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC)	1182:1257	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	80	theme	high	1440:1443	arg1	content					1460:1466	a high total phenolic content	1438:1466	a high total phenolic content (850.62 mg GAE·100 g-1)	1438:1490	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	80	theme	high	1440:1443	arg1	GAE·100 g-1					1479:1489	850.62 mg GAE·100 g-1	1469:1489	850.62 mg GAE·100 g-1	1469:1489	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	8	81	theme	Probiotic	1703:1711	arg1	microorganisms					1713:1726	Probiotic microorganisms	1703:1726	Probiotic microorganisms	1703:1726	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	3	82	theme	pulp	759:762	arg1	potential					720:728	the prebiotic potential	706:728	the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC)	706:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	6	83	theme	strains	1540:1546	arg1	reduction					1571:1579	a more relevant pH reduction	1552:1579	a more relevant pH reduction when compared to the commercial prebiotic FOS	1552:1625	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	6	83	theme	strains	1540:1546	arg1	growth					1520:1525	a better growth	1511:1525	a better growth of probiotic strains	1511:1546	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	1	84	dep	jerivá	130:135	arg1	romanzoffiana					146:158	Syagrus romanzoffiana	138:158	Syagrus romanzoffiana	138:158	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	5	85	theme	macaúba	1141:1147	arg1	pulps					1149:1153	Jerivá and macaúba pulps	1130:1153	Jerivá and macaúba pulps	1130:1153	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	86	theme	phenolic	1451:1458	arg1	content					1460:1466	a high total phenolic content	1438:1466	a high total phenolic content (850.62 mg GAE·100 g-1)	1438:1490	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	86	theme	phenolic	1451:1458	arg1	GAE·100 g-1					1479:1489	850.62 mg GAE·100 g-1	1469:1489	850.62 mg GAE·100 g-1	1469:1489	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	0	87	theme	pulp	23:26	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).	0:124	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	1	88	theme	family	230:235	arg1	trees					207:211	palm trees	202:211	palm trees	202:211	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	88	theme	family	230:235	arg1	macaúba					169:175	the macaúba	165:175	the macaúba (Acrocomia aculeata)	165:196	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	1	88	theme	family	230:235	arg1	jerivá					130:135	The jerivá	126:135	The jerivá (Syagrus romanzoffiana)	126:159	The jerivá (Syagrus romanzoffiana) and the macaúba (Acrocomia aculeata) are palm trees of the Arecaceae family, widely distributed in tropical and subtropical areas of Latin America, which have a low production cost and high productivity throughout the year.					
32846620	5	89	dep	DPPH	1358:1361	arg1	fruit					1385:1389	2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH	1364:1416	fruit	1385:1389	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	89	dep	DPPH	1358:1361	arg1	DPPH					1413:1416	2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH	1364:1416	DPPH	1413:1416	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	3	90	theme	kernel	777:782	arg1	JC					790:791	JC	790:791	JC	790:791	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	3	90	theme	kernel	777:782	arg1	cake					784:787	jerivá kernel cake	770:787	jerivá kernel cake (JC)	770:792	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	9	91	theme	macaúba	1934:1940	arg1	extraction					1946:1955	macaúba oil extraction	1934:1955	macaúba oil extraction	1934:1955	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32846620	0	92	theme	kernel	32:37	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).	0:124	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	2	93	theme	high	396:399	arg1	content					401:407	the high content	392:407	the high content of lipids	392:417	Due to the high content of lipids, their fruits have been used for oil extraction, which generates byproducts such as the pulps and the kernel cakes, a nutritionally rich byproduct that can be added into human food and, may have prebiotic potential.					
32846620	6	94	theme	better	1513:1518	arg1	growth					1520:1525	a better growth	1511:1525	a better growth of probiotic strains	1511:1546	Also, JP promoted a better growth of probiotic strains and a more relevant pH reduction when compared to the commercial prebiotic FOS.					
32846620	5	95	dep	levels	1187:1192	arg1	%					1217:1217	20.45%	1212:1217	20.45% JP	1212:1220	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	95	dep	levels	1187:1192	arg1	%					1253:1253	35.81%	1248:1253	35.81% MC	1248:1256	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	95	dep	levels	1187:1192	arg1	JP					1219:1220	JP	1219:1220	JP	1219:1220	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	95	dep	levels	1187:1192	arg1	%					1228:1228	37.87% JC	1223:1231	37.87% JC	1223:1231	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	95	dep	levels	1187:1192	arg1	%					1239:1239	19.95%	1234:1239	19.95% MP	1234:1242	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	8	96	theme	short-chain	1777:1787	arg1	acids					1795:1799	short-chain fatty acids	1777:1799	short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits	1777:1889	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	8	96	theme	short-chain	1777:1787	arg1	acid					1848:1851	lactic, propionic, butyric, and acetic acid	1809:1851	lactic, propionic, butyric, and acetic acid	1809:1851	Probiotic microorganisms were able to use JP, MP, JC, and MC and produced short-chain fatty acids such as lactic, propionic, butyric, and acetic acid, capable of promoting health benefits.					
32846620	4	97	theme	acidophilus	1092:1102	arg1	capacity					1017:1024	the antimicrobial and fermentative capacity	982:1024	capacity	1017:1024	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	0	98	dep	pulp	23:26	arg1	cake					39:42	cake	39:42	cake	39:42	Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata).					
32846620	3	99	theme	cake	813:816	arg1	potential					720:728	the prebiotic potential	706:728	the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC)	706:821	Therefore, the objective of this work was to characterize and evaluate the prebiotic potential of jerivá pulp (JP), macaúba pulp (MP), jerivá kernel cake (JC) and macaúba kernel cake (MC).					
32846620	4	100	theme	proximate	886:894	arg1	composition					896:906	proximate composition	886:906	proximate composition	886:906	For this, the fruits characterization was carried out through proximate composition, phenolic compounds content, and antioxidant activity, besides evaluating the antimicrobial and fermentative capacity of Bifidobacterium lactis, Lactobacillus casei, and Lactobacillus acidophilus against Escherichia coli.					
32846620	5	101	theme	fiber	1205:1209	arg1	activity					1280:1287	high antioxidant activity	1263:1287	high antioxidant activity	1263:1287	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	5	101	theme	fiber	1205:1209	arg1	levels					1187:1192	high levels	1182:1192	high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC)	1182:1257	Jerivá and macaúba pulps and kernel cakes presented high levels of dietary fiber (20.45% JP, 37.87% JC, 19.95% MP and 35.81% MC) and high antioxidant activity, especially JP, which also showed the high values found for ABTS and DPPH (2498.49 µMTrolox·g-1 fruit and 96.97 g fruit·g-1 DPPH, respectively), has a high total phenolic content (850.62 mg GAE·100 g-1).					
32846620	9	102	theme	prebiotic	1998:2006	arg1	components					2041:2050	their prebiotic potential, and maybe interesting components	1992:2050	their prebiotic potential, and maybe interesting components	1992:2050	Therefore, the byproducts from jerivá and macaúba oil extraction have characteristics that indicate their prebiotic potential, and maybe interesting components to increase the nutritional value of foods.					
32213407	0	0	theme	dye	68:70	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	1	from	removal	9:15	arg1	metals					57:62	heavy metals	51:62	heavy metals	51:62	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	1	from	removal	9:15	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	2	theme	%	664:664	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	2	3	theme	dye	492:494	arg1	co-stresses					460:470	the co-stresses	456:470	the co-stresses of heavy metals and dye	456:494	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	7	4	theme	continuous	1458:1467	arg1	treatment					1480:1488	continuous wastewater treatment	1458:1488	continuous wastewater treatment under HMs-MO co-contaminant	1458:1516	In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.					
32213407	1	5	theme	wastewater	272:281	arg1	treatment					283:291	wastewater treatment	272:291	wastewater treatment	272:291	Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment.					
32213407	0	6	theme	wastewater	88:97	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	7	from	Fate	0:3	arg1	metals					57:62	heavy metals	51:62	heavy metals	51:62	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	7	from	Fate	0:3	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	6	8	theme	bacteria	1298:1305	arg1	reduction					1254:1262	the reduction	1250:1262	the reduction of potential antibiotic-resistant bacteria	1250:1305	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	6	8	theme	bacteria	1298:1305	arg1	driver					1326:1331	a driver	1324:1331	a driver of ARGs removal	1324:1347	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	3	9	theme	heavy	541:545	arg1	HMs					555:557	HMs	555:557	HMs	555:557	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	9	theme	heavy	541:545	arg1	metals					547:552	20 mg/L heavy metals	533:552	20 mg/L heavy metals (HMs)	533:558	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	4	10	theme	tet	965:967	arg1	M					969:969	tet M	965:969	tet M	965:969	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	3	11	theme	%	758:758	arg1	that					770:773	that	770:773	that	770:773	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	11	theme	%	758:758	arg1	%					765:765	87.92%-94.11%	753:765	87.92%-94.11% of that in control	753:784	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	0	12	theme	co-contaminated	72:86	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	13	theme	MO	673:674	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	5	14	theme	β-BC	1106:1109	arg1	presence					1111:1118	β-BC presence	1106:1118	β-BC presence	1106:1118	The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive.					
32213407	7	15	theme	HMs-MO	1496:1501	arg1	co-contaminant					1503:1516	HMs-MO co-contaminant	1496:1516	HMs-MO co-contaminant	1496:1516	In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.					
32213407	7	16	theme	ARGs	1446:1449	arg1	removal					1435:1441	the removal	1431:1441	the removal of ARGs	1431:1449	In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.					
32213407	3	17	from	present	596:602	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	0	18	from	genes	42:46	arg1	metals					57:62	heavy metals	51:62	heavy metals	51:62	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	18	from	genes	42:46	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	19	from	efficiency	684:693	arg1	present					596:602	present	596:602	present	596:602	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	5	20	theme	ARGs	1082:1085	arg1	abundance					1054:1062	The relative abundance	1041:1062	The relative abundance of all identified ARGs	1041:1085	The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive.					
32213407	7	21	contain	possesses	1398:1406	arg1	β-BC					1393:1396	β-BC	1393:1396	β-BC	1393:1396	In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.					
32213407	7	21	contain	possesses	1398:1406	arg2	ability					1412:1418	the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant	1408:1516	the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant	1408:1516	In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.					
32213407	2	22	theme	genes	413:417	arg1	removal					380:386	the removal	376:386	the removal of antibiotic resistance genes (ARGs)	376:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	0	23	theme	treatment	99:107	arg1	system					109:114	dye co-contaminated wastewater treatment system	68:114	dye co-contaminated wastewater treatment system	68:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	24	theme	-98.84	665:670	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	2	25	theme	metals	481:486	arg1	co-stresses					460:470	the co-stresses	456:470	the co-stresses of heavy metals and dye	456:494	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	3	26	theme	20 mg/L	533:539	arg1	HMs					555:557	HMs	555:557	HMs	555:557	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	26	theme	20 mg/L	533:539	arg1	metals					547:552	20 mg/L heavy metals	533:552	20 mg/L heavy metals (HMs)	533:558	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	2	27	theme	resistance	402:411	arg1	ARGs					420:423	ARGs	420:423	ARGs	420:423	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	2	27	theme	resistance	402:411	arg1	genes					413:417	antibiotic resistance genes	391:417	antibiotic resistance genes (ARGs)	391:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	2	28	theme	heavy	475:479	arg1	metals					481:486	heavy metals	475:486	heavy metals	475:486	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	3	29	theme	%	671:671	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	2	30	theme	β-cyclodextrin	328:341	arg1	β-BC					367:370	β-BC	367:370	β-BC	367:370	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	2	30	theme	β-cyclodextrin	328:341	arg1	biochar					358:364	β-cyclodextrin functionalized biochar	328:364	β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs)	328:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	3	31	theme	removal	676:682	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	2	32	theme	antibiotic	391:400	arg1	ARGs					420:423	ARGs	420:423	ARGs	420:423	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	2	32	theme	antibiotic	391:400	arg1	genes					413:417	antibiotic resistance genes	391:417	antibiotic resistance genes (ARGs)	391:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	3	33	from	%	765:765	arg1	control					778:784	control	778:784	control	778:784	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	6	34	theme	microbial	1213:1221	arg1	community					1223:1231	microbial community	1213:1231	microbial community	1213:1231	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	2	35	from	removal	380:386	arg1	performance					313:323	the performance	309:323	the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye	309:494	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	4	36	theme	stressed	941:948	arg1	system					950:955	multi-contaminants stressed system	922:955	multi-contaminants stressed system	922:955	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	3	37	theme	contaminants	854:865	arg1	removal					867:873	contaminants removal	854:873	contaminants removal	854:873	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	4	38	theme	tet	958:960	arg1	W					962:962	tet W	958:962	tet W	958:962	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	3	39	theme	removal	867:873	arg1	performance					839:849	the performance	835:849	the performance of contaminants removal	835:873	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	1	40	from	treatment	283:291	arg1	promising					259:267	promising	259:267	promising	259:267	Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment.					
32213407	4	41	from	ARGs	914:917	arg1	qnr-S					1001:1005	qnr-S	1001:1005	qnr-S	1001:1005	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	W					962:962	tet W	958:962	tet W	958:962	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	role					892:895	the role	888:895	the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1	888:1023	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	erm-B					1008:1012	erm-B	1008:1012	erm-B	1008:1012	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	M					969:969	tet M	965:969	tet M	965:969	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	blaTEM					986:991	blaTEM	986:991	blaTEM	986:991	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	system					950:955	multi-contaminants stressed system	922:955	multi-contaminants stressed system	922:955	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	oxa-1					994:998	oxa-1	994:998	oxa-1	994:998	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	intI-1					1018:1023	intI-1	1018:1023	intI-1	1018:1023	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	sul-1					972:976	sul-1	972:976	sul-1	972:976	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	4	41	from	ARGs	914:917	arg1	sul-2					979:983	sul-2	979:983	sul-2	979:983	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	3	42	from	control	778:784	arg1	that					770:773	that	770:773	that	770:773	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	42	from	control	778:784	arg1	%					765:765	87.92%-94.11%	753:765	87.92%-94.11% of that in control	753:784	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	2	43	from	biochar	358:364	arg1	removal					380:386	the removal	376:386	the removal of antibiotic resistance genes (ARGs)	376:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	4	44	dep	β-BC	900:903	arg1	plays					905:909	plays	905:909	plays	905:909	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	2	45	theme	wastewater	429:438	arg1	treatment					440:448	wastewater treatment	429:448	wastewater treatment	429:448	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	5	46	theme	relative	1045:1052	arg1	abundance					1054:1062	The relative abundance	1041:1062	The relative abundance of all identified ARGs	1041:1085	The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive.					
32213407	0	47	theme	resistance	31:40	arg1	genes					42:46	antibiotic resistance genes	20:46	antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system	20:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	48	theme	functionalized	144:157	arg1	biochar					159:165	β-cyclodextrin functionalized biochar	129:165	β-cyclodextrin functionalized biochar	129:165	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	49	theme	influent	617:624	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	5	50	theme	corresponding	1136:1148	arg1	groups					1150:1155	the corresponding groups	1132:1155	the corresponding groups without β-BC additive	1132:1177	The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive.					
32213407	1	51	theme	environmental	228:240	arg1	effect					242:247	its environmental effect	224:247	its environmental effect	224:247	Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment.					
32213407	0	52	theme	antibiotic	20:29	arg1	genes					42:46	antibiotic resistance genes	20:46	antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system	20:114	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	4	53	theme	β-BC	900:903	arg1	role					892:895	the role	888:895	the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1	888:1023	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	0	54	theme	β-cyclodextrin	129:142	arg1	biochar					159:165	β-cyclodextrin functionalized biochar	129:165	β-cyclodextrin functionalized biochar	129:165	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	55	from	metals	57:62	arg1	removal					9:15	removal	9:15	removal	9:15	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	55	from	metals	57:62	arg1	Fate					0:3	Fate	0:3	Fate	0:3	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	6	56	dep	diversity	1184:1192	arg1	The					1180:1182	The	1180:1182	The	1180:1182	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	6	57	theme	community	1223:1231	arg1	composition					1198:1208	composition	1198:1208	composition	1198:1208	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	6	57	theme	community	1223:1231	arg1	diversity					1184:1192	diversity	1184:1192	diversity	1184:1192	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	4	58	theme	multi-contaminants	922:939	arg1	system					950:955	multi-contaminants stressed system	922:955	multi-contaminants stressed system	922:955	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	3	59	theme	β-BC	707:710	arg1	group					721:725	β-BC additive group	707:725	β-BC additive group	707:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	60	theme	150 mg/L	564:571	arg1	orange					580:585	150 mg/L methyl orange	564:585	150 mg/L methyl orange (MO)	564:590	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	60	theme	150 mg/L	564:571	arg1	MO					588:589	MO	588:589	MO	588:589	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	6	61	dep	ARGs	1336:1339	arg1	removal					1341:1347	removal	1341:1347	ARGs removal	1336:1347	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	0	62	theme	genes	42:46	arg1	removal					9:15	removal	9:15	removal	9:15	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	62	theme	genes	42:46	arg1	Fate					0:3	Fate	0:3	Fate	0:3	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	63	theme	additive	712:719	arg1	group					721:725	β-BC additive group	707:725	β-BC additive group	707:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	1	64	from	promising	259:267	arg1	treatment					283:291	wastewater treatment	272:291	wastewater treatment	272:291	Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment.					
32213407	0	65	from	system	109:114	arg1	removal					9:15	removal	9:15	removal	9:15	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	0	65	from	system	109:114	arg1	Fate					0:3	Fate	0:3	Fate	0:3	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	66	theme	methyl	573:578	arg1	orange					580:585	150 mg/L methyl orange	564:585	150 mg/L methyl orange (MO)	564:590	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	66	theme	methyl	573:578	arg1	MO					588:589	MO	588:589	MO	588:589	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	67	attach	present	596:602	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	67	attach	present	596:602	arg2	metals					547:552	20 mg/L heavy metals	533:552	20 mg/L heavy metals (HMs)	533:558	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	67	attach	present	596:602	arg2	HMs					555:557	HMs	555:557	HMs	555:557	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	67	attach	present	596:602	arg2	MO					588:589	MO	588:589	MO	588:589	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	67	attach	present	596:602	arg2	orange					580:585	150 mg/L methyl orange	564:585	150 mg/L methyl orange (MO)	564:590	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	4	68	from	β-BC	900:903	arg1	ARGs					914:917	ARGs	914:917	ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1	914:1023	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	5	69	theme	identified	1071:1080	arg1	ARGs					1082:1085	all identified ARGs	1067:1085	all identified ARGs	1067:1085	The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive.					
32213407	2	70	theme	biochar	358:364	arg1	performance					313:323	the performance	309:323	the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye	309:494	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	7	71	theme	wastewater	1469:1478	arg1	treatment					1480:1488	continuous wastewater treatment	1458:1488	continuous wastewater treatment under HMs-MO co-contaminant	1458:1516	In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.					
32213407	2	72	theme	functionalized	343:356	arg1	β-BC					367:370	β-BC	367:370	β-BC	367:370	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	2	72	theme	functionalized	343:356	arg1	biochar					358:364	β-cyclodextrin functionalized biochar	328:364	β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs)	328:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	3	73	theme	that	770:773	arg1	that					770:773	that	770:773	that	770:773	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	73	theme	that	770:773	arg1	%					765:765	87.92%-94.11%	753:765	87.92%-94.11% of that in control	753:784	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	74	located	present	596:602	arg1	efficiency					684:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency	607:693	daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group	607:725	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	74	located	present	596:602	arg2	metals					547:552	20 mg/L heavy metals	533:552	20 mg/L heavy metals (HMs)	533:558	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	74	located	present	596:602	arg2	HMs					555:557	HMs	555:557	HMs	555:557	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	74	located	present	596:602	arg2	MO					588:589	MO	588:589	MO	588:589	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	3	74	located	present	596:602	arg2	orange					580:585	150 mg/L methyl orange	564:585	150 mg/L methyl orange (MO)	564:590	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	6	75	theme	potential	1267:1275	arg1	bacteria					1298:1305	potential antibiotic-resistant bacteria	1267:1305	potential antibiotic-resistant bacteria	1267:1305	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	6	76	theme	ARGs	1336:1339	arg1	reduction					1254:1262	the reduction	1250:1262	the reduction of potential antibiotic-resistant bacteria	1250:1305	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	6	76	theme	ARGs	1336:1339	arg1	driver					1326:1331	a driver	1324:1331	a driver of ARGs removal	1324:1347	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	6	77	theme	antibiotic-resistant	1277:1296	arg1	bacteria					1298:1305	potential antibiotic-resistant bacteria	1267:1305	potential antibiotic-resistant bacteria	1267:1305	The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal.					
32213407	0	78	theme	heavy	51:55	arg1	metals					57:62	heavy metals	51:62	heavy metals	51:62	Fate and removal of antibiotic resistance genes in heavy metals and dye co-contaminated wastewater treatment system amended with β-cyclodextrin functionalized biochar.					
32213407	3	79	theme	96.79	659:663	arg1	%					664:664	%	664:664	%	664:664	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32213407	4	80	from	role	892:895	arg1	ARGs					914:917	ARGs	914:917	ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1	914:1023	To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified.					
32213407	2	81	from	performance	313:323	arg1	treatment					440:448	wastewater treatment	429:448	wastewater treatment	429:448	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	2	81	from	performance	313:323	arg1	removal					380:386	the removal	376:386	the removal of antibiotic resistance genes (ARGs)	376:424	In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated.					
32213407	3	82	dep	influent	617:624	arg1	residual					646:653	residual	646:653	residual	646:653	Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal.					
32094354	12	0	theme	temperature	1148:1158	arg1	biocarbon					1160:1168	The higher temperature biocarbon	1137:1168	The higher temperature biocarbon	1137:1168	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	2	1	used	used	249:252	arg2	materials					212:220	these waste materials	200:220	these waste materials	200:220	Currently, these waste materials are discarded to landfill, used as animal feed or incinerated.					
32094354	2	1	used	used	249:252	arg2	feed					264:267	animal feed	257:267	animal feed	257:267	Currently, these waste materials are discarded to landfill, used as animal feed or incinerated.					
32094354	10	2	dep	48	1104:1105	arg1	to					1101:1102	to	1101:1102	to	1101:1102	The tensile and flexural moduli improved with the addition of biocarbon, and the bio-content increased from 35 to 48 wt.					
32094354	5	3	theme	surface	573:579	arg1	morphology					581:590	their unique surface morphology	560:590	their unique surface morphology	560:590	Peanut hulls were pyrolyzed at 500 °C and analyzed to discover their unique surface morphology and relatively low ash content.					
32094354	1	4	theme	tons	131:134	arg1	millions					119:126	millions	119:126	millions of tons of post-food processing residues discarded annually	119:186	There are millions of tons of post-food processing residues discarded annually.					
32094354	4	5	theme	composites	485:494	arg1	generation					465:474	the generation	461:474	the generation of green composites	461:494	This work focuses on the characterization and valorization of peanut hulls through the generation of green composites.					
32094354	8	6	theme	trimethylene	918:929	arg1	poly					913:916	poly	913:916	poly(trimethylene terephthalate) (PTT)	913:950	Partially biobased engineering thermoplastic, poly(trimethylene terephthalate) (PTT), was combined with 20 wt.					
32094354	8	6	theme	trimethylene	918:929	arg1	terephthalate					931:943	trimethylene terephthalate	918:943	trimethylene terephthalate	918:943	Partially biobased engineering thermoplastic, poly(trimethylene terephthalate) (PTT), was combined with 20 wt.					
32094354	14	7	theme	electrical	1508:1517	arg1	housings					1519:1526	electrical housings	1508:1526	electrical housings	1508:1526	Future works could focus on the optimization of these materials for non-structural automotive components or electrical housings.					
32094354	2	8	theme	animal	257:262	arg1	materials					212:220	these waste materials	200:220	these waste materials	200:220	Currently, these waste materials are discarded to landfill, used as animal feed or incinerated.					
32094354	2	8	theme	animal	257:262	arg1	feed					264:267	animal feed	257:267	animal feed	257:267	Currently, these waste materials are discarded to landfill, used as animal feed or incinerated.					
32094354	13	9	theme	enhanced	1248:1255	arg1	sustainability					1257:1270	The enhanced sustainability	1244:1270	The enhanced sustainability of these materials	1244:1289	The enhanced sustainability of these materials suggested that peanut hulls can be valorized via thermochemical conversion to generate value-added products.					
32094354	0	10	theme	trimethylene	74:85	arg1	poly					69:72	biobased poly	60:72	biobased poly(trimethylene terephthalate) (PTT)	60:106	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	0	10	theme	trimethylene	74:85	arg1	terephthalate					87:99	trimethylene terephthalate	74:99	trimethylene terephthalate	74:99	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	0	11	from	composites	44:53	arg1	Biocarbon					0:8	Biocarbon	0:8	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).	0:107	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	12	12	theme	higher	1141:1146	arg1	biocarbon					1160:1168	The higher temperature biocarbon	1137:1168	The higher temperature biocarbon	1137:1168	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	7	13	theme	002	844:846	arg1	planes					859:864	(002) and (100) planes	843:864	planes	859:864	Such results were confirmed in X-ray diffraction analysis by the presence of (002) and (100) planes.					
32094354	4	14	theme	hulls	447:451	arg1	valorization					424:435	valorization	424:435	valorization	424:435	This work focuses on the characterization and valorization of peanut hulls through the generation of green composites.					
32094354	4	14	theme	hulls	447:451	arg1	characterization					403:418	characterization	403:418	characterization	403:418	This work focuses on the characterization and valorization of peanut hulls through the generation of green composites.					
32094354	5	15	theme	low	607:609	arg1	content					615:621	relatively low ash content	596:621	relatively low ash content	596:621	Peanut hulls were pyrolyzed at 500 °C and analyzed to discover their unique surface morphology and relatively low ash content.					
32094354	7	16	theme	planes	859:864	arg1	presence					831:838	the presence	827:838	the presence of (002) and (100) planes	827:864	Such results were confirmed in X-ray diffraction analysis by the presence of (002) and (100) planes.					
32094354	14	17	theme	Future	1400:1405	arg1	works					1407:1411	Future works	1400:1411	Future works	1400:1411	Future works could focus on the optimization of these materials for non-structural automotive components or electrical housings.					
32094354	12	18	theme	lower	1218:1222	arg1	sample					1236:1241	the lower temperature sample	1214:1241	the lower temperature sample	1214:1241	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	5	19	theme	ash	611:613	arg1	content					615:621	relatively low ash content	596:621	relatively low ash content	596:621	Peanut hulls were pyrolyzed at 500 °C and analyzed to discover their unique surface morphology and relatively low ash content.					
32094354	1	20	theme	post-food	139:147	arg1	residues					160:167	post-food processing residues	139:167	post-food processing residues discarded annually	139:186	There are millions of tons of post-food processing residues discarded annually.					
32094354	13	21	theme	thermochemical	1340:1353	arg1	conversion					1355:1364	thermochemical conversion	1340:1364	thermochemical conversion	1340:1364	The enhanced sustainability of these materials suggested that peanut hulls can be valorized via thermochemical conversion to generate value-added products.					
32094354	14	22	theme	automotive	1483:1492	arg1	components					1494:1503	non-structural automotive components	1468:1503	non-structural automotive components	1468:1503	Future works could focus on the optimization of these materials for non-structural automotive components or electrical housings.					
32094354	11	23	theme	neat	1127:1130	arg1	PTT					1132:1134	neat PTT	1127:1134	neat PTT	1127:1134	% as compared to neat PTT.					
32094354	3	24	dep	potential	314:322	arg1	uses					324:327	uses	324:327	uses	324:327	This suggests that there are potential uses for these materials in value-added applications.					
32094354	1	25	theme	processing	149:158	arg1	residues					160:167	post-food processing residues	139:167	post-food processing residues discarded annually	139:186	There are millions of tons of post-food processing residues discarded annually.					
32094354	3	26	theme	value-added	352:362	arg1	applications					364:375	value-added applications	352:375	value-added applications	352:375	This suggests that there are potential uses for these materials in value-added applications.					
32094354	7	27	theme	diffraction	803:813	arg1	analysis					815:822	X-ray diffraction analysis	797:822	X-ray diffraction analysis	797:822	Such results were confirmed in X-ray diffraction analysis by the presence of (002) and (100) planes.					
32094354	14	28	theme	non-structural	1468:1481	arg1	components					1494:1503	non-structural automotive components	1468:1503	non-structural automotive components	1468:1503	Future works could focus on the optimization of these materials for non-structural automotive components or electrical housings.					
32094354	6	29	theme	0.74	675:678	arg1	values					665:670	ID/IG values	659:670	ID/IG values of 0.74 for the samples	659:694	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
32094354	1	30	theme	residues	160:167	arg1	tons					131:134	tons	131:134	tons of post-food processing residues discarded annually	131:186	There are millions of tons of post-food processing residues discarded annually.					
32094354	5	31	theme	unique	566:571	arg1	morphology					581:590	their unique surface morphology	560:590	their unique surface morphology	560:590	Peanut hulls were pyrolyzed at 500 °C and analyzed to discover their unique surface morphology and relatively low ash content.					
32094354	12	32	theme	temperature	1224:1234	arg1	sample					1236:1241	the lower temperature sample	1214:1241	the lower temperature sample	1214:1241	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	12	33	theme	superior	1188:1195	arg1	performance					1197:1207	superior performance	1188:1207	superior performance	1188:1207	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	0	34	theme	peanut	15:20	arg1	hulls					22:26	peanut hulls	15:26	peanut hulls	15:26	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	13	35	theme	value-added	1378:1388	arg1	products					1390:1397	value-added products	1378:1397	value-added products	1378:1397	The enhanced sustainability of these materials suggested that peanut hulls can be valorized via thermochemical conversion to generate value-added products.					
32094354	8	36	theme	biobased	877:884	arg1	poly					913:916	poly	913:916	poly(trimethylene terephthalate) (PTT)	913:950	Partially biobased engineering thermoplastic, poly(trimethylene terephthalate) (PTT), was combined with 20 wt.					
32094354	8	36	theme	biobased	877:884	arg1	thermoplastic					898:910	Partially biobased engineering thermoplastic	867:910	Partially biobased engineering thermoplastic	867:910	Partially biobased engineering thermoplastic, poly(trimethylene terephthalate) (PTT), was combined with 20 wt.					
32094354	8	37	theme	engineering	886:896	arg1	poly					913:916	poly	913:916	poly(trimethylene terephthalate) (PTT)	913:950	Partially biobased engineering thermoplastic, poly(trimethylene terephthalate) (PTT), was combined with 20 wt.					
32094354	8	37	theme	engineering	886:896	arg1	thermoplastic					898:910	Partially biobased engineering thermoplastic	867:910	Partially biobased engineering thermoplastic	867:910	Partially biobased engineering thermoplastic, poly(trimethylene terephthalate) (PTT), was combined with 20 wt.					
32094354	7	38	theme	X-ray	797:801	arg1	analysis					815:822	X-ray diffraction analysis	797:822	X-ray diffraction analysis	797:822	Such results were confirmed in X-ray diffraction analysis by the presence of (002) and (100) planes.					
32094354	3	39	from	potential	314:322	arg1	applications					364:375	value-added applications	352:375	value-added applications	352:375	This suggests that there are potential uses for these materials in value-added applications.					
32094354	6	40	theme	ID/IG	659:663	arg1	values					665:670	ID/IG values	659:670	ID/IG values of 0.74 for the samples	659:694	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
32094354	4	41	theme	peanut	440:445	arg1	hulls					447:451	peanut hulls	440:451	peanut hulls	440:451	This work focuses on the characterization and valorization of peanut hulls through the generation of green composites.					
32094354	2	42	theme	waste	206:210	arg1	materials					212:220	these waste materials	200:220	these waste materials	200:220	Currently, these waste materials are discarded to landfill, used as animal feed or incinerated.					
32094354	2	42	theme	waste	206:210	arg1	feed					264:267	animal feed	257:267	animal feed	257:267	Currently, these waste materials are discarded to landfill, used as animal feed or incinerated.					
32094354	13	43	theme	peanut	1306:1311	arg1	hulls					1313:1317	peanut hulls	1306:1317	peanut hulls	1306:1317	The enhanced sustainability of these materials suggested that peanut hulls can be valorized via thermochemical conversion to generate value-added products.					
32094354	0	44	theme	green	38:42	arg1	composites					44:53	their green composites	32:53	their green composites	32:53	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	7	45	theme	Such	766:769	arg1	results					771:777	Such results	766:777	Such results	766:777	Such results were confirmed in X-ray diffraction analysis by the presence of (002) and (100) planes.					
32094354	0	46	from	hulls	22:26	arg1	Biocarbon					0:8	Biocarbon	0:8	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).	0:107	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	10	47	theme	flexural	1006:1013	arg1	moduli					1015:1020	The tensile and flexural moduli	990:1020	The tensile and flexural moduli	990:1020	The tensile and flexural moduli improved with the addition of biocarbon, and the bio-content increased from 35 to 48 wt.					
32094354	6	48	theme	disordered carbon	739:755	arg1	content					757:763	disordered carbon content	739:763	disordered carbon content	739:763	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
32094354	12	49	contain	have	1183:1186	arg1	biocarbon					1160:1168	The higher temperature biocarbon	1137:1168	The higher temperature biocarbon	1137:1168	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	12	49	contain	have	1183:1186	arg2	performance					1197:1207	superior performance	1188:1207	superior performance	1188:1207	The higher temperature biocarbon was found to have superior performance over the lower temperature sample.					
32094354	14	50	theme	materials	1454:1462	arg1	optimization					1432:1443	the optimization	1428:1443	the optimization of these materials for non-structural automotive components or electrical housings	1428:1526	Future works could focus on the optimization of these materials for non-structural automotive components or electrical housings.					
32094354	0	51	with	Biocarbon	0:8	arg1	poly					69:72	biobased poly	60:72	biobased poly(trimethylene terephthalate) (PTT)	60:106	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	0	51	with	Biocarbon	0:8	arg1	terephthalate					87:99	trimethylene terephthalate	74:99	trimethylene terephthalate	74:99	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	0	51	with	Biocarbon	0:8	arg1	PTT					103:105	PTT	103:105	PTT	103:105	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	10	52	theme	biocarbon	1052:1060	arg1	addition					1040:1047	the addition	1036:1047	the addition of biocarbon	1036:1060	The tensile and flexural moduli improved with the addition of biocarbon, and the bio-content increased from 35 to 48 wt.					
32094354	9	53	theme	%	977:977	arg1	biocarbon					979:987	% biocarbon	977:987	% biocarbon.	977:988	% biocarbon.					
32094354	4	54	dep	characterization	403:418	arg1	the					399:401	the	399:401	the	399:401	This work focuses on the characterization and valorization of peanut hulls through the generation of green composites.					
32094354	10	55	theme	tensile	994:1000	arg1	moduli					1015:1020	The tensile and flexural moduli	990:1020	The tensile and flexural moduli	990:1020	The tensile and flexural moduli improved with the addition of biocarbon, and the bio-content increased from 35 to 48 wt.					
32094354	5	56	theme	Peanut	497:502	arg1	hulls					504:508	Peanut hulls	497:508	Peanut hulls	497:508	Peanut hulls were pyrolyzed at 500 °C and analyzed to discover their unique surface morphology and relatively low ash content.					
32094354	6	57	theme	spectral	630:637	arg1	analysis					639:646	Raman spectral analysis	624:646	Raman spectral analysis	624:646	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
32094354	0	58	theme	biobased	60:67	arg1	poly					69:72	biobased poly	60:72	biobased poly(trimethylene terephthalate) (PTT)	60:106	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	0	58	theme	biobased	60:67	arg1	terephthalate					87:99	trimethylene terephthalate	74:99	trimethylene terephthalate	74:99	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	0	58	theme	biobased	60:67	arg1	PTT					103:105	PTT	103:105	PTT	103:105	Biocarbon from peanut hulls and their green composites with biobased poly(trimethylene terephthalate) (PTT).					
32094354	6	59	theme	graphitic	716:724	arg1	content					726:732	greater graphitic content	708:732	greater graphitic content than disordered carbon content	708:763	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
32094354	6	60	theme	Raman	624:628	arg1	analysis					639:646	Raman spectral analysis	624:646	Raman spectral analysis	624:646	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
32094354	13	61	theme	materials	1281:1289	arg1	sustainability					1257:1270	The enhanced sustainability	1244:1270	The enhanced sustainability of these materials	1244:1289	The enhanced sustainability of these materials suggested that peanut hulls can be valorized via thermochemical conversion to generate value-added products.					
32094354	4	62	theme	green	479:483	arg1	composites					485:494	green composites	479:494	green composites	479:494	This work focuses on the characterization and valorization of peanut hulls through the generation of green composites.					
32094354	6	63	theme	greater	708:714	arg1	content					726:732	greater graphitic content	708:732	greater graphitic content than disordered carbon content	708:763	Raman spectral analysis determined ID/IG values of 0.74 for the samples, suggesting greater graphitic content than disordered carbon content.					
31943702	0	0	theme	biomedical	107:116	arg1	applications					118:129	biomedical applications	107:129	biomedical applications	107:129	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	1	1	dep	bone	310:313	arg1	engineering					335:345	tissue engineering	328:345	tissue engineering	328:345	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	2	2	theme	bacteria	478:485	arg1	growth					487:492	bacteria growth	478:492	bacteria growth on its surface, which limits its applications in biomedical fields	478:559	However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields.					
31943702	7	3	theme	native	1199:1204	arg1	BC					1206:1207	native BC	1199:1207	native BC	1199:1207	Furthermore, after grafting with APTES, the membranes got less hydrophilic than native BC.					
31943702	4	4	theme	alkoxysilane	791:802	arg1	polycondensation					804:819	alkoxysilane polycondensation	791:819	alkoxysilane polycondensation using 3-aminopropyltriethoxysilane (APTES)	791:862	In the present study, aminoalkyl-grafted BC membranes were prepared by alkoxysilane polycondensation using 3-aminopropyltriethoxysilane (APTES).					
31943702	3	5	theme	special	687:693	arg1	applications					706:717	some special biomedical applications	682:717	some special biomedical applications	682:717	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	3	6	theme	chemical	589:596	arg1	modification					598:609	chemical modification	589:609	chemical modification of BC membranes	589:625	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	4	7	theme	aminoalkyl-grafted	742:759	arg1	membranes					764:772	aminoalkyl-grafted BC membranes	742:772	aminoalkyl-grafted BC membranes	742:772	In the present study, aminoalkyl-grafted BC membranes were prepared by alkoxysilane polycondensation using 3-aminopropyltriethoxysilane (APTES).					
31943702	8	8	theme	BC	1233:1234	arg1	membranes					1236:1244	The aminoalkyl-grafted BC membranes	1210:1244	The aminoalkyl-grafted BC membranes	1210:1244	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	1	9	theme	skin	367:370	arg1	repairing					372:380	skin repairing	367:380	skin repairing	367:380	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	5	10	theme	aminoalkylsilane	982:997	arg1	groups					999:1004	aminoalkylsilane groups	982:1004	aminoalkylsilane groups	982:1004	The characterization for morphology and chemical composition showed that BC membranes were successfully grafted with aminoalkylsilane groups through covalent bonding.					
31943702	8	11	theme	strong	1253:1258	arg1	properties					1274:1283	strong antibacterial properties	1253:1283	strong antibacterial properties	1253:1283	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	8	12	theme	antibacterial	1260:1272	arg1	properties					1274:1283	strong antibacterial properties	1253:1283	strong antibacterial properties	1253:1283	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	5	13	theme	chemical	905:912	arg1	composition					914:924	chemical composition	905:924	chemical composition	905:924	The characterization for morphology and chemical composition showed that BC membranes were successfully grafted with aminoalkylsilane groups through covalent bonding.					
31943702	7	14	dep	hydrophilic	1182:1192	arg1	got					1173:1175	got	1173:1175	got	1173:1175	Furthermore, after grafting with APTES, the membranes got less hydrophilic than native BC.					
31943702	9	15	theme	aminoalkyl-grafted	1433:1450	arg1	membranes					1455:1463	aminoalkyl-grafted BC membranes	1433:1463	aminoalkyl-grafted BC membranes	1433:1463	These results indicate that aminoalkyl-grafted BC membranes are potential to be used for biomedical applications.					
31943702	9	15	theme	aminoalkyl-grafted	1433:1450	arg1	potential					1469:1477	potential	1469:1477	potential	1469:1477	These results indicate that aminoalkyl-grafted BC membranes are potential to be used for biomedical applications.					
31943702	8	16	theme	aminoalkyl-grafted	1214:1231	arg1	membranes					1236:1244	The aminoalkyl-grafted BC membranes	1210:1244	The aminoalkyl-grafted BC membranes	1210:1244	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	0	17	theme	bacterial	34:42	arg1	membranes					54:62	aminoalkyl-grafted bacterial cellulose membranes	15:62	aminoalkyl-grafted bacterial cellulose membranes	15:62	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	6	18	theme	surface	1036:1042	arg1	morphology					1044:1053	surface morphology	1036:1053	surface morphology	1036:1053	The surface morphology and roughness of the membranes changed after chemical grafting.					
31943702	2	19	theme	biomedical	543:552	arg1	fields					554:559	biomedical fields	543:559	biomedical fields	543:559	However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields.					
31943702	1	20	theme	Bacterial	132:140	arg1	membranes					157:165	Bacterial cellulose (BC) membranes	132:165	Bacterial cellulose (BC) membranes	132:165	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	3	21	from	reports	578:584	arg1	modification					598:609	chemical modification	589:609	chemical modification of BC membranes	589:625	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	1	22	theme	much	226:229	arg1	attention					231:239	much attention	226:239	much attention	226:239	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	0	23	theme	aminoalkyl-grafted	15:32	arg1	membranes					54:62	aminoalkyl-grafted bacterial cellulose membranes	15:62	aminoalkyl-grafted bacterial cellulose membranes	15:62	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	2	24	from	growth	487:492	arg1	surface					501:507	its surface	497:507	its surface	497:507	However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields.					
31943702	1	25	theme	cellulose	142:150	arg1	membranes					157:165	Bacterial cellulose (BC) membranes	132:165	Bacterial cellulose (BC) membranes	132:165	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	0	26	theme	membranes	54:62	arg1	Preparation					0:10	Preparation	0:10	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.	0:130	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	8	27	theme	human	1379:1383	arg1	fibroblasts					1392:1402	normal human dermal fibroblasts	1372:1402	normal human dermal fibroblasts	1372:1402	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	8	28	theme	normal	1372:1377	arg1	fibroblasts					1392:1402	normal human dermal fibroblasts	1372:1402	normal human dermal fibroblasts	1372:1402	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	0	29	theme	cellulose	44:52	arg1	membranes					54:62	aminoalkyl-grafted bacterial cellulose membranes	15:62	aminoalkyl-grafted bacterial cellulose membranes	15:62	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	2	30	theme	native	438:443	arg1	BC					445:446	native BC	438:446	native BC	438:446	However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields.					
31943702	0	31	theme	improved	69:76	arg1	properties					92:101	improved antimicrobial properties	69:101	improved antimicrobial properties for biomedical applications	69:129	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	1	32	theme	controlled	403:412	arg1	delivery					419:426	controlled drug delivery	403:426	controlled drug delivery	403:426	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	1	33	from	application	244:254	arg1	areas					274:278	the biomedical areas	259:278	the biomedical areas	259:278	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	8	34	theme	dermal	1385:1390	arg1	fibroblasts					1392:1402	normal human dermal fibroblasts	1372:1402	normal human dermal fibroblasts	1372:1402	The aminoalkyl-grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts.					
31943702	5	35	theme	covalent	1014:1021	arg1	bonding					1023:1029	covalent bonding	1014:1029	covalent bonding	1014:1029	The characterization for morphology and chemical composition showed that BC membranes were successfully grafted with aminoalkylsilane groups through covalent bonding.					
31943702	1	36	theme	tissue	328:333	arg1	engineering					335:345	tissue engineering	328:345	tissue engineering	328:345	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	1	37	theme	drug	414:417	arg1	delivery					419:426	controlled drug delivery	403:426	controlled drug delivery	403:426	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	1	38	theme	BC	153:154	arg1	membranes					157:165	Bacterial cellulose (BC) membranes	132:165	Bacterial cellulose (BC) membranes	132:165	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	3	39	theme	BC	614:615	arg1	membranes					617:625	BC membranes	614:625	BC membranes	614:625	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	0	40	with	Preparation	0:10	arg1	properties					92:101	improved antimicrobial properties	69:101	improved antimicrobial properties for biomedical applications	69:129	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	4	41	theme	present	727:733	arg1	study					735:739	the present study	723:739	the present study	723:739	In the present study, aminoalkyl-grafted BC membranes were prepared by alkoxysilane polycondensation using 3-aminopropyltriethoxysilane (APTES).					
31943702	4	42	theme	BC	761:762	arg1	membranes					764:772	aminoalkyl-grafted BC membranes	742:772	aminoalkyl-grafted BC membranes	742:772	In the present study, aminoalkyl-grafted BC membranes were prepared by alkoxysilane polycondensation using 3-aminopropyltriethoxysilane (APTES).					
31943702	6	43	theme	chemical	1100:1107	arg1	grafting					1109:1116	chemical grafting	1100:1116	chemical grafting	1100:1116	The surface morphology and roughness of the membranes changed after chemical grafting.					
31943702	3	44	theme	antimicrobial	646:658	arg1	properties					660:669	antimicrobial properties	646:669	antimicrobial properties needed for some special biomedical applications	646:717	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	3	45	theme	membranes	617:625	arg1	modification					598:609	chemical modification	589:609	chemical modification of BC membranes	589:625	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	9	46	theme	BC	1452:1453	arg1	membranes					1455:1463	aminoalkyl-grafted BC membranes	1433:1463	aminoalkyl-grafted BC membranes	1433:1463	These results indicate that aminoalkyl-grafted BC membranes are potential to be used for biomedical applications.					
31943702	9	46	theme	BC	1452:1453	arg1	potential					1469:1477	potential	1469:1477	potential	1469:1477	These results indicate that aminoalkyl-grafted BC membranes are potential to be used for biomedical applications.					
31943702	5	47	theme	BC	938:939	arg1	membranes					941:949	BC membranes	938:949	BC membranes	938:949	The characterization for morphology and chemical composition showed that BC membranes were successfully grafted with aminoalkylsilane groups through covalent bonding.					
31943702	9	48	theme	biomedical	1494:1503	arg1	applications					1505:1516	biomedical applications	1494:1516	biomedical applications	1494:1516	These results indicate that aminoalkyl-grafted BC membranes are potential to be used for biomedical applications.					
31943702	1	49	theme	biomedical	263:272	arg1	areas					274:278	the biomedical areas	259:278	the biomedical areas	259:278	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	0	50	theme	antimicrobial	78:90	arg1	properties					92:101	improved antimicrobial properties	69:101	improved antimicrobial properties for biomedical applications	69:129	Preparation of aminoalkyl-grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications.					
31943702	3	51	theme	biomedical	695:704	arg1	applications					706:717	some special biomedical applications	682:717	some special biomedical applications	682:717	There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications.					
31943702	1	52	theme	special	175:181	arg1	structures					198:207	structures	198:207	structures	198:207	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	1	52	theme	special	175:181	arg1	properties					183:192	special properties	175:192	special properties	175:192	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	1	52	theme	special	175:181	arg1	implants					297:304	implants	297:304	implants for bone or cartilage tissue engineering	297:345	Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery.					
31943702	6	53	theme	membranes	1076:1084	arg1	roughness					1059:1067	roughness	1059:1067	roughness	1059:1067	The surface morphology and roughness of the membranes changed after chemical grafting.					
31943702	6	53	theme	membranes	1076:1084	arg1	morphology					1044:1053	surface morphology	1036:1053	surface morphology	1036:1053	The surface morphology and roughness of the membranes changed after chemical grafting.					
33050843	11	0	theme	complex	1322:1328	arg1	landscape					1294:1302	the free energy landscape	1278:1302	the free energy landscape of mutant SAA1-HS complex	1278:1328	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	2	1	theme	protein	336:342	arg1	aggregation					279:289	the aggregation	275:289	the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	275:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	12	2	from	amyloidosis	1630:1640	arg1	conclusion					1489:1498	conclusion	1489:1498	conclusion	1489:1498	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	11	3	theme	global	1364:1369	arg1	conformers					1378:1387	multiple global minima conformers	1355:1387	multiple global minima conformers	1355:1387	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	11	4	theme	SAA1	1472:1475	arg1	protein					1477:1483	native SAA1 protein	1465:1483	native SAA1 protein	1465:1483	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	10	5	theme	beta	1213:1216	arg1	turn					1218:1221	beta turn	1213:1221	beta turn	1213:1221	Further, the complex showed increase of beta turn in its secondary structural composition.					
33050843	2	6	from	protein	336:342	arg1	organs					367:372	organs	367:372	organs	367:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	2	6	from	protein	336:342	arg1	tissues					355:361	various tissues	347:361	various tissues	347:361	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	11	7	theme	free	1282:1285	arg1	landscape					1294:1302	the free energy landscape	1278:1302	the free energy landscape of mutant SAA1-HS complex	1278:1328	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	9	8	theme	fluctuating	1149:1159	arg1	trajectory					1161:1170	a fluctuating trajectory	1147:1170	a fluctuating trajectory	1147:1170	Also, both RMSD and Rg evinced a fluctuating trajectory.					
33050843	2	9	theme	serum	312:316	arg1	protein					336:342	mutated/misfolded serum amyloid A1 (SAA1) protein	294:342	mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	294:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	1	10	theme	pathogenic	178:187	arg1	amyloidosis					159:169	Amyloid-A mediated (AA) amyloidosis	135:169	Amyloid-A mediated (AA) amyloidosis	135:169	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	1	10	theme	pathogenic	178:187	arg1	byproduct					189:197	the pathogenic byproduct	174:197	the pathogenic byproduct of body's prolonged exposure to inflammatory conditions	174:253	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	0	11	theme	bioinformatics	110:123	arg1	approach					125:132	a structural bioinformatics approach	97:132	a structural bioinformatics approach	97:132	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	11	12	attach	presence	1407:1414	arg2	conformation					1449:1460	a single global energy minima conformation	1419:1460	a single global energy minima conformation	1419:1460	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	11	12	attach	presence	1407:1414	arg1	protein					1477:1483	native SAA1 protein	1465:1483	native SAA1 protein	1465:1483	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	11	13	theme	mutant	1307:1312	arg1	complex					1322:1328	mutant SAA1-HS complex	1307:1328	mutant SAA1-HS complex	1307:1328	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	5	14	theme	pernicious	702:711	arg1	modifications					713:725	the pernicious modifications	698:725	the pernicious modifications in G90D mutant SAA1 protein	698:753	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	5	15	from	modifications	713:725	arg1	protein					747:753	G90D mutant SAA1 protein	730:753	G90D mutant SAA1 protein	730:753	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	12	16	theme	conformational	1520:1533	arg1	ramifications					1535:1547	the aforementioned conformational ramifications	1501:1547	the aforementioned conformational ramifications induced by HS on SAA1	1501:1569	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	12	16	theme	conformational	1520:1533	arg1	amyloidosis					1630:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	5	17	theme	HS	664:665	arg1	binding					653:659	the enhanced binding	640:659	the enhanced binding of HS	640:665	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	7	18	theme	augmented	983:991	arg1	association					993:1003	an augmented association	980:1003	an augmented association with HS	980:1011	Accordingly, the protein-ligand binding studies indicate that upon SNP G90D, SAA1 protein exhibited an augmented association with HS.					
33050843	11	19	theme	energy	1435:1440	arg1	conformation					1449:1460	a single global energy minima conformation	1419:1460	a single global energy minima conformation	1419:1460	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	1	20	theme	Amyloid-A	135:143	arg1	amyloidosis					159:169	Amyloid-A mediated (AA) amyloidosis	135:169	Amyloid-A mediated (AA) amyloidosis	135:169	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	1	20	theme	Amyloid-A	135:143	arg1	AA					155:156	AA	155:156	AA	155:156	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	1	20	theme	Amyloid-A	135:143	arg1	byproduct					189:197	the pathogenic byproduct	174:197	the pathogenic byproduct of body's prolonged exposure to inflammatory conditions	174:253	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	2	21	from	aggregation	279:289	arg1	organs					367:372	organs	367:372	organs	367:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	2	21	from	aggregation	279:289	arg1	tissues					355:361	various tissues	347:361	various tissues	347:361	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	11	22	theme	single	1421:1426	arg1	conformation					1449:1460	a single global energy minima conformation	1419:1460	a single global energy minima conformation	1419:1460	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	0	23	theme	serum	59:63	arg1	aggregation					84:94	serum amyloid A1 protein aggregation	59:94	serum amyloid A1 protein aggregation	59:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	7	24	theme	SAA1	957:960	arg1	protein					962:968	SAA1 protein	957:968	SAA1 protein	957:968	Accordingly, the protein-ligand binding studies indicate that upon SNP G90D, SAA1 protein exhibited an augmented association with HS.					
33050843	4	25	theme	Recent	486:491	arg1	findings					506:513	Recent experimental findings	486:513	Recent experimental findings	486:513	Recent experimental findings insinuate that heparan sulphate (HS), a glycosaminoglycans, exhibits binding with SAA1 to promote its aggregation.					
33050843	11	26	theme	native	1465:1470	arg1	protein					1477:1483	native SAA1 protein	1465:1483	native SAA1 protein	1465:1483	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	0	27	theme	A1	73:74	arg1	aggregation					84:94	serum amyloid A1 protein aggregation	59:94	serum amyloid A1 protein aggregation	59:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	7	28	with	association	993:1003	arg1	HS					1010:1011	HS	1010:1011	HS	1010:1011	Accordingly, the protein-ligand binding studies indicate that upon SNP G90D, SAA1 protein exhibited an augmented association with HS.					
33050843	11	29	theme	conformation	1449:1460	arg1	presence					1407:1414	the presence	1403:1414	the presence of a single global energy minima conformation in native SAA1 protein	1403:1483	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	12	30	theme	behind	1620:1625	arg1	ramifications					1535:1547	the aforementioned conformational ramifications	1501:1547	the aforementioned conformational ramifications induced by HS on SAA1	1501:1569	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	12	30	theme	behind	1620:1625	arg1	amyloidosis					1630:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	3	31	theme	AA	423:424	arg1	amyloidosis					426:436	AA amyloidosis	423:436	AA amyloidosis	423:436	Genetic polymorphism G90D is suspected to cause AA amyloidosis, although the causal mechanism remains cryptic.					
33050843	5	32	theme	mutant	735:740	arg1	protein					747:753	G90D mutant SAA1 protein	730:753	G90D mutant SAA1 protein	730:753	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	6	33	from	influence	778:786	arg1	conformation					809:820	the dynamic conformation	797:820	the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis	797:877	Also, we examined the influence of HS on the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis.					
33050843	0	34	theme	amyloid	65:71	arg1	aggregation					84:94	serum amyloid A1 protein aggregation	59:94	serum amyloid A1 protein aggregation	59:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	11	35	theme	conformers	1378:1387	arg1	occurrence					1341:1350	the occurrence	1337:1350	the occurrence of multiple global minima conformers	1337:1387	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	8	36	theme	HS	1041:1042	arg1	complex					1062:1068	HS bound mutant SAA1 complex	1041:1068	HS bound mutant SAA1 complex	1041:1068	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	7	37	theme	binding	912:918	arg1	studies					920:926	the protein-ligand binding studies	893:926	the protein-ligand binding studies	893:926	Accordingly, the protein-ligand binding studies indicate that upon SNP G90D, SAA1 protein exhibited an augmented association with HS.					
33050843	0	38	theme	pernicious	18:27	arg1	effects					29:35	the pernicious effects	14:35	the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation	14:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	8	39	theme	mutant	1050:1055	arg1	complex					1062:1068	HS bound mutant SAA1 complex	1041:1068	HS bound mutant SAA1 complex	1041:1068	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	12	40	theme	proteopathic	1596:1607	arg1	ramifications					1535:1547	the aforementioned conformational ramifications	1501:1547	the aforementioned conformational ramifications induced by HS on SAA1	1501:1569	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	12	40	theme	proteopathic	1596:1607	arg1	amyloidosis					1630:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	10	41	theme	secondary	1230:1238	arg1	composition					1251:1261	its secondary structural composition	1226:1261	its secondary structural composition	1226:1261	Further, the complex showed increase of beta turn in its secondary structural composition.					
33050843	8	42	theme	complex	1062:1068	arg1	simulation					1027:1036	the simulation	1023:1036	the simulation of HS bound mutant SAA1 complex	1023:1068	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	6	43	theme	SAA1	832:835	arg1	conformation					809:820	the dynamic conformation	797:820	the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis	797:877	Also, we examined the influence of HS on the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis.					
33050843	2	44	from	organs	367:372	arg1	aggregation					279:289	the aggregation	275:289	the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	275:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	3	45	theme	polymorphism	383:394	arg1	G90D					396:399	Genetic polymorphism G90D	375:399	Genetic polymorphism G90D	375:399	Genetic polymorphism G90D is suspected to cause AA amyloidosis, although the causal mechanism remains cryptic.					
33050843	0	46	theme	sulfate	48:54	arg1	effects					29:35	the pernicious effects	14:35	the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation	14:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	0	47	from	effects	29:35	arg1	aggregation					84:94	serum amyloid A1 protein aggregation	59:94	serum amyloid A1 protein aggregation	59:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	7	48	theme	protein-ligand	897:910	arg1	studies					920:926	the protein-ligand binding studies	893:926	the protein-ligand binding studies	893:926	Accordingly, the protein-ligand binding studies indicate that upon SNP G90D, SAA1 protein exhibited an augmented association with HS.					
33050843	1	49	theme	prolonged	209:217	arg1	exposure					219:226	body's prolonged exposure	202:226	body's prolonged exposure to inflammatory conditions	202:253	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	6	50	theme	dynamic	801:807	arg1	conformation					809:820	the dynamic conformation	797:820	the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis	797:877	Also, we examined the influence of HS on the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis.					
33050843	0	51	dep	Investigating	0:12	arg1	approach					125:132	a structural bioinformatics approach	97:132	a structural bioinformatics approach	97:132	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	11	52	theme	SAA1-HS	1314:1320	arg1	complex					1322:1328	mutant SAA1-HS complex	1307:1328	mutant SAA1-HS complex	1307:1328	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	12	53	theme	effective	1719:1727	arg1	therapeutics					1729:1740	effective therapeutics	1719:1740	effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma	1719:1798	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	11	54	theme	multiple	1355:1362	arg1	conformers					1378:1387	multiple global minima conformers	1355:1387	multiple global minima conformers	1355:1387	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	5	55	theme	enhanced	644:651	arg1	binding					653:659	the enhanced binding	640:659	the enhanced binding of HS	640:665	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	6	56	theme	HS	791:792	arg1	influence					778:786	the influence	774:786	the influence of HS on the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis	774:877	Also, we examined the influence of HS on the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis.					
33050843	12	57	theme	future	1689:1694	arg1	studies					1696:1702	future studies	1689:1702	future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma	1689:1798	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	2	58	theme	amyloid	318:324	arg1	protein					336:342	mutated/misfolded serum amyloid A1 (SAA1) protein	294:342	mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	294:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	2	59	from	tissues	355:361	arg1	aggregation					279:289	the aggregation	275:289	the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	275:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	2	60	theme	mutated/misfolded	294:310	arg1	protein					336:342	mutated/misfolded serum amyloid A1 (SAA1) protein	294:342	mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	294:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	10	61	from	increase	1201:1208	arg1	composition					1251:1261	its secondary structural composition	1226:1261	its secondary structural composition	1226:1261	Further, the complex showed increase of beta turn in its secondary structural composition.					
33050843	11	62	theme	energy	1287:1292	arg1	landscape					1294:1302	the free energy landscape	1278:1302	the free energy landscape of mutant SAA1-HS complex	1278:1328	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	11	63	theme	global	1428:1433	arg1	conformation					1449:1460	a single global energy minima conformation	1419:1460	a single global energy minima conformation	1419:1460	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	4	64	with	binding	584:590	arg1	SAA1					597:600	SAA1	597:600	SAA1	597:600	Recent experimental findings insinuate that heparan sulphate (HS), a glycosaminoglycans, exhibits binding with SAA1 to promote its aggregation.					
33050843	5	65	theme	SAA1	742:745	arg1	protein					747:753	G90D mutant SAA1 protein	730:753	G90D mutant SAA1 protein	730:753	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	1	66	theme	mediated	145:152	arg1	amyloidosis					159:169	Amyloid-A mediated (AA) amyloidosis	135:169	Amyloid-A mediated (AA) amyloidosis	135:169	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	1	66	theme	mediated	145:152	arg1	AA					155:156	AA	155:156	AA	155:156	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	1	66	theme	mediated	145:152	arg1	byproduct					189:197	the pathogenic byproduct	174:197	the pathogenic byproduct of body's prolonged exposure to inflammatory conditions	174:253	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
33050843	12	67	theme	aforementioned	1505:1518	arg1	ramifications					1535:1547	the aforementioned conformational ramifications	1501:1547	the aforementioned conformational ramifications induced by HS on SAA1	1501:1569	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	12	67	theme	aforementioned	1505:1518	arg1	amyloidosis					1630:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	4	68	theme	experimental	493:504	arg1	findings					506:513	Recent experimental findings	486:513	Recent experimental findings	486:513	Recent experimental findings insinuate that heparan sulphate (HS), a glycosaminoglycans, exhibits binding with SAA1 to promote its aggregation.					
33050843	0	69	theme	protein	76:82	arg1	aggregation					84:94	serum amyloid A1 protein aggregation	59:94	serum amyloid A1 protein aggregation	59:94	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	11	70	theme	minima	1442:1447	arg1	conformation					1449:1460	a single global energy minima conformation	1419:1460	a single global energy minima conformation	1419:1460	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	8	71	from	increase	1084:1091	arg1	Rg					1102:1103	Rg	1102:1103	Rg	1102:1103	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	8	71	from	increase	1084:1091	arg1	RMSF					1110:1113	RMSF	1110:1113	RMSF	1110:1113	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	8	71	from	increase	1084:1091	arg1	RMSD					1096:1099	RMSD	1096:1099	RMSD	1096:1099	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	0	72	theme	structural	99:108	arg1	approach					125:132	a structural bioinformatics approach	97:132	a structural bioinformatics approach	97:132	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	7	73	theme	SNP	947:949	arg1	G90D					951:954	SNP G90D	947:954	SNP G90D	947:954	Accordingly, the protein-ligand binding studies indicate that upon SNP G90D, SAA1 protein exhibited an augmented association with HS.					
33050843	12	74	theme	incendiary	1609:1618	arg1	ramifications					1535:1547	the aforementioned conformational ramifications	1501:1547	the aforementioned conformational ramifications induced by HS on SAA1	1501:1569	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	12	74	theme	incendiary	1609:1618	arg1	amyloidosis					1630:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	10	75	theme	turn	1218:1221	arg1	increase					1201:1208	increase	1201:1208	increase of beta turn in its secondary structural composition	1201:1261	Further, the complex showed increase of beta turn in its secondary structural composition.					
33050843	5	76	theme	G90D	730:733	arg1	protein					747:753	G90D mutant SAA1 protein	730:753	G90D mutant SAA1 protein	730:753	To foster the enhanced binding of HS, we computationally determined the pernicious modifications in G90D mutant SAA1 protein.					
33050843	8	77	theme	bound	1044:1048	arg1	complex					1062:1068	HS bound mutant SAA1 complex	1041:1068	HS bound mutant SAA1 complex	1041:1068	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	12	78	theme	AA	1627:1628	arg1	ramifications					1535:1547	the aforementioned conformational ramifications	1501:1547	the aforementioned conformational ramifications induced by HS on SAA1	1501:1569	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	12	78	theme	AA	1627:1628	arg1	amyloidosis					1630:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	the proteopathic incendiary behind AA amyloidosis	1592:1640	In conclusion, the aforementioned conformational ramifications induced by HS on SAA1 could potentially be the proteopathic incendiary behind AA amyloidosis; this incendiary will need to be considered in future studies for developing effective therapeutics against AA amyloidosis.Communicated by Ramaswamy H. Sarma.					
33050843	3	79	theme	Genetic	375:381	arg1	G90D					396:399	Genetic polymorphism G90D	375:399	Genetic polymorphism G90D	375:399	Genetic polymorphism G90D is suspected to cause AA amyloidosis, although the causal mechanism remains cryptic.					
33050843	8	80	theme	SAA1	1057:1060	arg1	complex					1062:1068	HS bound mutant SAA1 complex	1041:1068	HS bound mutant SAA1 complex	1041:1068	Further, the simulation of HS bound mutant SAA1 complex delineates an increase in RMSD, Rg, and RMSF.					
33050843	10	81	theme	structural	1240:1249	arg1	composition					1251:1261	its secondary structural composition	1226:1261	its secondary structural composition	1226:1261	Further, the complex showed increase of beta turn in its secondary structural composition.					
33050843	3	82	theme	causal	452:457	arg1	mechanism					459:467	the causal mechanism	448:467	the causal mechanism	448:467	Genetic polymorphism G90D is suspected to cause AA amyloidosis, although the causal mechanism remains cryptic.					
33050843	0	83	theme	heparan	40:46	arg1	sulfate					48:54	heparan sulfate	40:54	heparan sulfate	40:54	Investigating the pernicious effects of heparan sulfate in serum amyloid A1 protein aggregation: a structural bioinformatics approach.					
33050843	4	84	theme	heparan	530:536	arg1	sulphate					538:545	heparan sulphate	530:545	heparan sulphate (HS)	530:550	Recent experimental findings insinuate that heparan sulphate (HS), a glycosaminoglycans, exhibits binding with SAA1 to promote its aggregation.					
33050843	4	84	theme	heparan	530:536	arg1	glycosaminoglycans					555:572	a glycosaminoglycans	553:572	a glycosaminoglycans	553:572	Recent experimental findings insinuate that heparan sulphate (HS), a glycosaminoglycans, exhibits binding with SAA1 to promote its aggregation.					
33050843	4	84	theme	heparan	530:536	arg1	HS					548:549	HS	548:549	HS	548:549	Recent experimental findings insinuate that heparan sulphate (HS), a glycosaminoglycans, exhibits binding with SAA1 to promote its aggregation.					
33050843	6	85	theme	mutant	825:830	arg1	SAA1					832:835	mutant SAA1	825:835	mutant SAA1 that could potentially succor amyloidosis	825:877	Also, we examined the influence of HS on the dynamic conformation of mutant SAA1 that could potentially succor amyloidosis.					
33050843	11	86	from	presence	1407:1414	arg1	protein					1477:1483	native SAA1 protein	1465:1483	native SAA1 protein	1465:1483	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	2	87	theme	SAA1	330:333	arg1	protein					336:342	mutated/misfolded serum amyloid A1 (SAA1) protein	294:342	mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	294:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	2	88	theme	A1	326:327	arg1	protein					336:342	mutated/misfolded serum amyloid A1 (SAA1) protein	294:342	mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs	294:372	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	11	89	theme	minima	1371:1376	arg1	conformers					1378:1387	multiple global minima conformers	1355:1387	multiple global minima conformers	1355:1387	Additionally, the free energy landscape of mutant SAA1-HS complex posits the occurrence of multiple global minima conformers as opposed to the presence of a single global energy minima conformation in native SAA1 protein.					
33050843	2	90	theme	various	347:353	arg1	tissues					355:361	various tissues	347:361	various tissues	347:361	It is described by the aggregation of mutated/misfolded serum amyloid A1 (SAA1) protein in various tissues and organs.					
33050843	1	91	theme	inflammatory	231:242	arg1	conditions					244:253	inflammatory conditions	231:253	inflammatory conditions	231:253	Amyloid-A mediated (AA) amyloidosis is the pathogenic byproduct of body's prolonged exposure to inflammatory conditions.					
34286807	2	0	theme	TPP-WPI-DX	419:428	arg1	nm					453:454	135.26 and 193.64 nm	435:454	135.26 and 193.64 nm	435:454	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	2	0	theme	TPP-WPI-DX	419:428	arg1	nanocarriers					354:365	The nanocarriers	350:365	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX	350:428	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	5	1	theme	leukemia	1043:1050	arg1	cells					1052:1056	leukemia cells	1043:1056	leukemia cells	1043:1056	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	6	2	theme	promising	1130:1138	arg1	application					1140:1150	the promising application	1126:1150	the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin	1126:1226	The present study highlights the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin.					
34286807	0	3	theme	targeting	100:108	arg1	delivery					110:117	astaxanthin mitochondria targeting delivery	75:117	astaxanthin mitochondria targeting delivery	75:117	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	2	4	theme	astaxanthin-loaded	400:417	arg1	TPP-WPI-DX					419:428	astaxanthin-loaded TPP-WPI-DX	400:428	astaxanthin-loaded TPP-WPI-DX	400:428	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	3	5	from	stability	583:591	arg1	fluid					617:621	the stimulated blood fluid	596:621	the stimulated blood fluid	596:621	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	1	6	theme	self-assembly	280:292	arg1	method					294:299	self-assembly method	280:299	self-assembly method for astaxanthin mitochondria targeting delivery	280:347	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	3	7	theme	good	578:581	arg1	stability					583:591	good stability	578:591	good stability in the stimulated blood fluid	578:621	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	4	8	theme	TPP-modified	664:675	arg1	nanocarriers					677:688	the TPP-modified nanocarriers	660:688	the TPP-modified nanocarriers	660:688	In vitro experiments indicated that the TPP-modified nanocarriers could effectively realize lysosomes escape, and specifically accumulate in the cell mitochondria.					
34286807	5	9	theme	reactive	867:874	arg1	species					883:889	reactive oxygen species	867:889	reactive oxygen species generation produced from hydrogen peroxide	867:932	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	5	10	from	vitality	1031:1038	arg1	cells					1090:1094	mouse macrophage (RAW 264.7) cells	1061:1094	mouse macrophage (RAW 264.7) cells	1061:1094	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	3	11	theme	stimulated	600:609	arg1	fluid					617:621	the stimulated blood fluid	596:621	the stimulated blood fluid	596:621	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	4	12	theme	cell	769:772	arg1	mitochondria					774:785	the cell mitochondria	765:785	the cell mitochondria	765:785	In vitro experiments indicated that the TPP-modified nanocarriers could effectively realize lysosomes escape, and specifically accumulate in the cell mitochondria.					
34286807	6	13	theme	enhanced	1195:1202	arg1	delivery					1204:1211	enhanced delivery	1195:1211	enhanced delivery of astaxanthin	1195:1226	The present study highlights the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin.					
34286807	5	14	theme	oxygen	876:881	arg1	species					883:889	reactive oxygen species	867:889	reactive oxygen species generation produced from hydrogen peroxide	867:932	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	3	15	contain	had	574:576	arg1	nanocarriers					561:572	The mitochondria targeting nanocarriers	534:572	The mitochondria targeting nanocarriers	534:572	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	3	15	contain	had	574:576	arg2	stability					583:591	good stability	578:591	good stability in the stimulated blood fluid	578:621	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	5	16	theme	hydrogen	916:923	arg1	peroxide					925:932	hydrogen peroxide	916:932	hydrogen peroxide	916:932	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	3	17	theme	blood	611:615	arg1	fluid					617:621	the stimulated blood fluid	596:621	the stimulated blood fluid	596:621	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	1	18	theme	-modified	216:224	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	4	19	dep	In	624:625	arg1	vitro					627:631	vitro	627:631	vitro	627:631	In vitro experiments indicated that the TPP-modified nanocarriers could effectively realize lysosomes escape, and specifically accumulate in the cell mitochondria.					
34286807	5	20	theme	species	883:889	arg1	generation					891:900	reactive oxygen species generation	867:900	reactive oxygen species generation produced from hydrogen peroxide	867:932	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	6	21	theme	astaxanthin	1216:1226	arg1	delivery					1204:1211	enhanced delivery	1195:1211	enhanced delivery of astaxanthin	1195:1226	The present study highlights the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin.					
34286807	1	22	theme	whey	226:229	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	5	23	theme	normal	947:952	arg1	levels					954:959	the normal levels	943:959	the normal levels of the mitochondrial membrane potential	943:999	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	6	24	theme	present	1101:1107	arg1	study					1109:1113	The present study	1097:1113	The present study	1097:1113	The present study highlights the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin.					
34286807	1	25	theme	Novel	120:124	arg1	nanocarriers					149:160	Novel mitochondria targeting nanocarriers	120:160	Novel mitochondria targeting nanocarriers	120:160	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	1	26	theme	protein	231:237	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	2	27	theme	spherical	489:497	arg1	structure					499:507	a spherical structure	487:507	a spherical structure	487:507	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	1	28	theme	astaxanthin	305:315	arg1	delivery					340:347	astaxanthin mitochondria targeting delivery	305:347	astaxanthin mitochondria targeting delivery	305:347	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	5	29	theme	macrophage	1067:1076	arg1	cells					1090:1094	mouse macrophage (RAW 264.7) cells	1061:1094	mouse macrophage (RAW 264.7) cells	1061:1094	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	1	30	theme	mitochondria	126:137	arg1	nanocarriers					149:160	Novel mitochondria targeting nanocarriers	120:160	Novel mitochondria targeting nanocarriers	120:160	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	1	31	theme	isolate	239:245	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	1	32	theme	mitochondria	317:328	arg1	delivery					340:347	astaxanthin mitochondria targeting delivery	305:347	astaxanthin mitochondria targeting delivery	305:347	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	5	33	theme	astaxanthin-loaded	808:825	arg1	nanocarriers					827:838	the astaxanthin-loaded nanocarriers	804:838	the astaxanthin-loaded nanocarriers	804:838	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	1	34	theme	targeting	139:147	arg1	nanocarriers					149:160	Novel mitochondria targeting nanocarriers	120:160	Novel mitochondria targeting nanocarriers	120:160	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	5	35	theme	mitochondrial	968:980	arg1	potential					991:999	the mitochondrial membrane potential	964:999	the mitochondrial membrane potential	964:999	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	0	36	theme	protein	49:55	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	0	36	theme	protein	49:55	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	1	37	theme	targeting	330:338	arg1	delivery					340:347	astaxanthin mitochondria targeting delivery	305:347	astaxanthin mitochondria targeting delivery	305:347	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	5	38	theme	membrane	982:989	arg1	potential					991:999	the mitochondrial membrane potential	964:999	the mitochondrial membrane potential	964:999	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	1	39	theme	WPI	248:250	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	0	40	theme	glycosylated	36:47	arg1	protein					49:55	glycosylated protein	36:55	glycosylated protein	36:55	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	5	41	theme	potential	991:999	arg1	levels					954:959	the normal levels	943:959	the normal levels of the mitochondrial membrane potential	943:999	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	4	42	theme	In	624:625	arg1	experiments					633:643	In vitro experiments	624:643	In vitro experiments	624:643	In vitro experiments indicated that the TPP-modified nanocarriers could effectively realize lysosomes escape, and specifically accumulate in the cell mitochondria.					
34286807	5	43	theme	cells	1052:1056	arg1	vitality					1031:1038	the vitality	1027:1038	the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells	1027:1094	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	2	44	theme	good	513:516	arg1	dispersibility					518:531	good dispersibility	513:531	good dispersibility	513:531	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	2	45	theme	WPI-DX	389:394	arg1	nm					453:454	135.26 and 193.64 nm	435:454	135.26 and 193.64 nm	435:454	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	2	45	theme	WPI-DX	389:394	arg1	nanocarriers					354:365	The nanocarriers	350:365	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX	350:428	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	0	46	gly	glycosylated	36:47	arg1	protein					49:55	glycosylated protein	36:55	glycosylated protein	36:55	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	6	47	theme	mitochondria	1155:1166	arg1	application					1140:1150	the promising application	1126:1150	the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin	1126:1226	The present study highlights the promising application of mitochondria targeting nanocarriers for enhanced delivery of astaxanthin.					
34286807	2	48	theme	astaxanthin-loaded	370:387	arg1	WPI-DX					389:394	astaxanthin-loaded WPI-DX	370:394	astaxanthin-loaded WPI-DX	370:394	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	1	49	theme	-dextran	252:259	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	5	50	theme	mouse	1061:1065	arg1	RAW					1079:1081	RAW 264.7	1079:1087	RAW 264.7	1079:1087	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	5	50	theme	mouse	1061:1065	arg1	macrophage					1067:1076	mouse macrophage	1061:1076	mouse macrophage (RAW 264.7) cells	1061:1094	Simultaneously, the astaxanthin-loaded nanocarriers could significantly reduce reactive oxygen species generation produced from hydrogen peroxide, protect the normal levels of the mitochondrial membrane potential, and dramatically promote the vitality of leukemia cells in mouse macrophage (RAW 264.7) cells.					
34286807	3	51	theme	targeting	551:559	arg1	nanocarriers					561:572	The mitochondria targeting nanocarriers	534:572	The mitochondria targeting nanocarriers	534:572	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	3	52	theme	mitochondria	538:549	arg1	nanocarriers					561:572	The mitochondria targeting nanocarriers	534:572	The mitochondria targeting nanocarriers	534:572	The mitochondria targeting nanocarriers had good stability in the stimulated blood fluid.					
34286807	0	53	theme	mitochondria	87:98	arg1	delivery					110:117	astaxanthin mitochondria targeting delivery	75:117	astaxanthin mitochondria targeting delivery	75:117	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	1	54	theme	triphenylphosphonium	182:201	arg1	bromide					203:209	triphenylphosphonium bromide	182:209	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	1	54	theme	triphenylphosphonium	182:201	arg1	TPP					212:214	TPP	212:214	TPP	212:214	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	1	55	theme	DX	262:263	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
34286807	0	56	theme	astaxanthin	75:85	arg1	delivery					110:117	astaxanthin mitochondria targeting delivery	75:117	astaxanthin mitochondria targeting delivery	75:117	Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery.					
34286807	2	57	dep	nm	453:454	arg1	exhibited					477:485	exhibited	477:485	exhibited a spherical structure and good dispersibility	477:531	The nanocarriers of astaxanthin-loaded WPI-DX and astaxanthin-loaded TPP-WPI-DX were 135.26 and 193.64 nm, respectively, which exhibited a spherical structure and good dispersibility.					
34286807	1	58	theme	bromide	203:209	arg1	conjugates					266:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates	182:275	Novel mitochondria targeting nanocarriers were prepared using triphenylphosphonium bromide (TPP)-modified whey protein isolate (WPI)-dextran (DX) conjugates by self-assembly method for astaxanthin mitochondria targeting delivery.					
32553960	4	0	theme	de-ionized	580:589	arg1	water					591:595	alternate de-ionized water	570:595	alternate de-ionized water	570:595	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	8	1	theme	PNIPAM/CMC	969:978	arg1	hydrogels					990:998	the PNIPAM/CMC composite hydrogels	965:998	the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein	965:1067	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	8	2	with	hydrogels	990:998	arg1	performances					1040:1051	triple stimuli-responsive swelling performances	1005:1051	triple stimuli-responsive swelling performances	1005:1051	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	0	3	theme	swelling	127:134	arg1	performances					136:147	triple stimuli-responsive swelling performances	101:147	triple stimuli-responsive swelling performances	101:147	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	4	4	theme	swelling	549:556	arg1	behavior					558:565	reversible swelling behavior	538:565	reversible swelling behavior in alternate de-ionized water and NaCl solution	538:613	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	5	5	theme	mechanical	688:697	arg1	property					699:706	great compression mechanical property	670:706	great compression mechanical property	670:706	Moreover, the fabricated PNIPAM/CMC hydrogels display great compression mechanical property.					
32553960	5	6	theme	fabricated	630:639	arg1	hydrogels					652:660	the fabricated PNIPAM/CMC hydrogels	626:660	the fabricated PNIPAM/CMC hydrogels	626:660	Moreover, the fabricated PNIPAM/CMC hydrogels display great compression mechanical property.					
32553960	7	7	theme	loaded	831:836	arg1	hydrogels					849:857	TCH loaded PNIPAM/CMC hydrogels	827:857	TCH loaded PNIPAM/CMC hydrogels	827:857	TCH loaded PNIPAM/CMC hydrogels exhibit excellent antibacterial activity against E. coli and B. subtilis and good biocompatibility.					
32553960	4	8	theme	alternate	570:578	arg1	water					591:595	alternate de-ionized water	570:595	alternate de-ionized water	570:595	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	8	9	theme	stimuli-responsive	1012:1029	arg1	performances					1040:1051	triple stimuli-responsive swelling performances	1005:1051	triple stimuli-responsive swelling performances	1005:1051	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	7	10	theme	PNIPAM/CMC	838:847	arg1	hydrogels					849:857	TCH loaded PNIPAM/CMC hydrogels	827:857	TCH loaded PNIPAM/CMC hydrogels	827:857	TCH loaded PNIPAM/CMC hydrogels exhibit excellent antibacterial activity against E. coli and B. subtilis and good biocompatibility.					
32553960	2	11	theme	high	356:359	arg1	porosity					361:368	high porosity	356:368	high porosity	356:368	PNIPAM/CMC hydrogels exhibit 3D network structures with high porosity.					
32553960	8	12	theme	triple	1005:1010	arg1	performances					1040:1051	triple stimuli-responsive swelling performances	1005:1051	triple stimuli-responsive swelling performances	1005:1051	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	3	13	theme	swelling	494:501	arg1	performance					503:513	salt mutiple-responsive swelling performance	470:513	salt mutiple-responsive swelling performance	470:513	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	5	14	theme	compression	676:686	arg1	property					699:706	great compression mechanical property	670:706	great compression mechanical property	670:706	Moreover, the fabricated PNIPAM/CMC hydrogels display great compression mechanical property.					
32553960	6	15	theme	tetracycline	715:726	arg1	TCH					743:745	TCH	743:745	TCH	743:745	Then, tetracycline hydrochloride (TCH) was loaded into the PNIPAM/CMC hydrogels to endow their antibacterial ability.					
32553960	6	15	theme	tetracycline	715:726	arg1	hydrochloride					728:740	tetracycline hydrochloride	715:740	tetracycline hydrochloride (TCH)	715:746	Then, tetracycline hydrochloride (TCH) was loaded into the PNIPAM/CMC hydrogels to endow their antibacterial ability.					
32553960	3	16	theme	high	409:412	arg1	capacity					431:438	high water absorption capacity	409:438	high water absorption capacity	409:438	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	0	17	theme	stimuli-responsive	108:125	arg1	performances					136:147	triple stimuli-responsive swelling performances	101:147	triple stimuli-responsive swelling performances	101:147	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	6	18	theme	antibacterial	804:816	arg1	ability					818:824	their antibacterial ability	798:824	their antibacterial ability	798:824	Then, tetracycline hydrochloride (TCH) was loaded into the PNIPAM/CMC hydrogels to endow their antibacterial ability.					
32553960	0	19	theme	UV-mediated	0:10	arg1	synthesis					12:20	UV-mediated synthesis	0:20	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite	0:84	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	7	20	theme	good	936:939	arg1	biocompatibility					941:956	good biocompatibility	936:956	good biocompatibility	936:956	TCH loaded PNIPAM/CMC hydrogels exhibit excellent antibacterial activity against E. coli and B. subtilis and good biocompatibility.					
32553960	8	21	from	potentials	1080:1089	arg1	applications					1110:1121	many biomedical applications	1094:1121	many biomedical applications	1094:1121	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	0	22	theme	carboxymethyl	25:37	arg1	composite					76:84	carboxymethyl cellulose/poly-N-isopropylacrylamide composite	25:84	carboxymethyl cellulose/poly-N-isopropylacrylamide composite	25:84	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	4	23	from	behavior	558:565	arg1	water					591:595	alternate de-ionized water	570:595	alternate de-ionized water	570:595	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	4	23	from	behavior	558:565	arg1	solution					606:613	NaCl solution	601:613	NaCl solution	601:613	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	1	24	theme	physical	259:266	arg1	cross-linker					268:279	physical cross-linker	259:279	physical cross-linker by UV irradiation	259:297	Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels were prepared using nanoclay as physical cross-linker by UV irradiation.					
32553960	4	25	theme	reversible	538:547	arg1	behavior					558:565	reversible swelling behavior	538:565	reversible swelling behavior in alternate de-ionized water and NaCl solution	538:613	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	8	26	theme	great	1074:1078	arg1	potentials					1080:1089	great potentials	1074:1089	great potentials in many biomedical applications	1074:1121	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	8	27	theme	swelling	1031:1038	arg1	performances					1040:1051	triple stimuli-responsive swelling performances	1005:1051	triple stimuli-responsive swelling performances	1005:1051	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	0	28	theme	composite	76:84	arg1	synthesis					12:20	UV-mediated synthesis	0:20	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite	0:84	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	7	29	theme	TCH	827:829	arg1	hydrogels					849:857	TCH loaded PNIPAM/CMC hydrogels	827:857	TCH loaded PNIPAM/CMC hydrogels	827:857	TCH loaded PNIPAM/CMC hydrogels exhibit excellent antibacterial activity against E. coli and B. subtilis and good biocompatibility.					
32553960	0	30	theme	cellulose/poly-N-isopropylacrylamide	39:74	arg1	composite					76:84	carboxymethyl cellulose/poly-N-isopropylacrylamide composite	25:84	carboxymethyl cellulose/poly-N-isopropylacrylamide composite	25:84	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	2	31	theme	PNIPAM/CMC	300:309	arg1	hydrogels					311:319	PNIPAM/CMC hydrogels	300:319	PNIPAM/CMC hydrogels	300:319	PNIPAM/CMC hydrogels exhibit 3D network structures with high porosity.					
32553960	5	32	theme	PNIPAM/CMC	641:650	arg1	hydrogels					652:660	the fabricated PNIPAM/CMC hydrogels	626:660	the fabricated PNIPAM/CMC hydrogels	626:660	Moreover, the fabricated PNIPAM/CMC hydrogels display great compression mechanical property.					
32553960	1	33	theme	UV	284:285	arg1	irradiation					287:297	UV irradiation	284:297	UV irradiation	284:297	Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels were prepared using nanoclay as physical cross-linker by UV irradiation.					
32553960	3	34	theme	salt	470:473	arg1	performance					503:513	salt mutiple-responsive swelling performance	470:513	salt mutiple-responsive swelling performance	470:513	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	0	35	theme	triple	101:106	arg1	performances					136:147	triple stimuli-responsive swelling performances	101:147	triple stimuli-responsive swelling performances	101:147	UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.					
32553960	3	36	theme	mutiple-responsive	475:492	arg1	performance					503:513	salt mutiple-responsive swelling performance	470:513	salt mutiple-responsive swelling performance	470:513	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	8	37	contain	have	1069:1072	arg2	potentials					1080:1089	great potentials	1074:1089	great potentials in many biomedical applications	1074:1121	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	8	37	contain	have	1069:1072	arg1	hydrogels					990:998	the PNIPAM/CMC composite hydrogels	965:998	the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein	965:1067	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	8	38	theme	biomedical	1099:1108	arg1	applications					1110:1121	many biomedical applications	1094:1121	many biomedical applications	1094:1121	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	6	39	theme	PNIPAM/CMC	768:777	arg1	hydrogels					779:787	the PNIPAM/CMC hydrogels	764:787	the PNIPAM/CMC hydrogels	764:787	Then, tetracycline hydrochloride (TCH) was loaded into the PNIPAM/CMC hydrogels to endow their antibacterial ability.					
32553960	3	40	theme	PNIPAM/CMC	371:380	arg1	hydrogels					382:390	PNIPAM/CMC hydrogels	371:390	PNIPAM/CMC hydrogels	371:390	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	5	41	theme	great	670:674	arg1	property					699:706	great compression mechanical property	670:706	great compression mechanical property	670:706	Moreover, the fabricated PNIPAM/CMC hydrogels display great compression mechanical property.					
32553960	1	42	theme	/pPoly-N-isopropylacrylamide	179:206	arg1	hydrogels					217:225	Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels	150:225	Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels	150:225	Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels were prepared using nanoclay as physical cross-linker by UV irradiation.					
32553960	1	42	theme	/pPoly-N-isopropylacrylamide	179:206	arg1	PNIPAM					209:214	PNIPAM	209:214	PNIPAM	209:214	Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels were prepared using nanoclay as physical cross-linker by UV irradiation.					
32553960	8	43	theme	many	1094:1097	arg1	applications					1110:1121	many biomedical applications	1094:1121	many biomedical applications	1094:1121	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	2	44	theme	network	332:338	arg1	structures					340:349	3D network structures	329:349	3D network structures	329:349	PNIPAM/CMC hydrogels exhibit 3D network structures with high porosity.					
32553960	3	45	theme	water	414:418	arg1	capacity					431:438	high water absorption capacity	409:438	high water absorption capacity	409:438	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	2	46	theme	3D	329:330	arg1	structures					340:349	3D network structures	329:349	3D network structures	329:349	PNIPAM/CMC hydrogels exhibit 3D network structures with high porosity.					
32553960	7	47	theme	excellent	867:875	arg1	activity					891:898	excellent antibacterial activity	867:898	excellent antibacterial activity against E. coli and B. subtilis	867:930	TCH loaded PNIPAM/CMC hydrogels exhibit excellent antibacterial activity against E. coli and B. subtilis and good biocompatibility.					
32553960	3	48	theme	absorption	420:429	arg1	capacity					431:438	high water absorption capacity	409:438	high water absorption capacity	409:438	PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and salt mutiple-responsive swelling performance.					
32553960	4	49	theme	NaCl	601:604	arg1	solution					606:613	NaCl solution	601:613	NaCl solution	601:613	Besides, they exhibit reversible swelling behavior in alternate de-ionized water and NaCl solution.					
32553960	8	50	theme	composite	980:988	arg1	hydrogels					990:998	the PNIPAM/CMC composite hydrogels	965:998	the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein	965:1067	Thus, the PNIPAM/CMC composite hydrogels with triple stimuli-responsive swelling performances reported herein have great potentials in many biomedical applications.					
32553960	7	51	theme	antibacterial	877:889	arg1	activity					891:898	excellent antibacterial activity	867:898	excellent antibacterial activity against E. coli and B. subtilis	867:930	TCH loaded PNIPAM/CMC hydrogels exhibit excellent antibacterial activity against E. coli and B. subtilis and good biocompatibility.					
32791272	3	0	theme	Cellulose	366:374	arg1	CNF					389:391	CNF	389:391	CNF	389:391	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	3	0	theme	Cellulose	366:374	arg1	nanofibrous					376:386	Cellulose nanofibrous	366:386	Cellulose nanofibrous (CNF)	366:392	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	6	1	theme	7-135 μm	938:945	arg1	size					930:933	the pore size	921:933	the pore size	921:933	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	10	2	theme	antimicrobial	1527:1539	arg1	curcumin					1512:1519	curcumin	1512:1519	curcumin	1512:1519	Antimicrobial property was also achieved in the optimal scaffold by incorporating curcumin as an antimicrobial agent.					
32791272	10	2	theme	antimicrobial	1527:1539	arg1	agent					1541:1545	an antimicrobial agent	1524:1545	an antimicrobial agent	1524:1545	Antimicrobial property was also achieved in the optimal scaffold by incorporating curcumin as an antimicrobial agent.					
32791272	8	3	theme	61.35 kPa	1243:1251	arg1	strength					1231:1238	the highest compressive strength	1207:1238	the highest compressive strength of 61.35 kPa	1207:1251	The formulation of crosslinked CNF-GEL 90:10 offered the highest compressive strength of 61.35 kPa.					
32791272	7	4	theme	compressive	1098:1108	arg1	strength					1110:1117	the compressive strength	1094:1117	the compressive strength	1094:1117	The presence of EPH improved the chemical interaction between CNF and gelatine, hence enhanced the compressive strength compared to uncrosslinked samples.					
32791272	9	5	theme	in-vivo	1271:1277	arg1	studies					1279:1285	The in-vitro and in-vivo studies	1254:1285	The in-vitro and in-vivo studies	1254:1285	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	7	6	theme	uncrosslinked	1131:1143	arg1	samples					1145:1151	uncrosslinked samples	1131:1151	uncrosslinked samples	1131:1151	The presence of EPH improved the chemical interaction between CNF and gelatine, hence enhanced the compressive strength compared to uncrosslinked samples.					
32791272	9	7	theme	cell	1322:1325	arg1	viability					1327:1335	cell viability	1322:1335	cell viability	1322:1335	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	6	8	theme	pore	925:928	arg1	size					930:933	the pore size	921:933	the pore size	921:933	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	9	9	link	crosslinked	1372:1382	arg1	formulation					1384:1394	the optimal crosslinked formulation	1360:1394	the optimal crosslinked formulation with tuned enzymatic degradation	1360:1427	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	8	10	theme	highest	1211:1217	arg1	strength					1231:1238	the highest compressive strength	1207:1238	the highest compressive strength of 61.35 kPa	1207:1251	The formulation of crosslinked CNF-GEL 90:10 offered the highest compressive strength of 61.35 kPa.					
32791272	6	11	contain	possessed	889:897	arg2	porosity					899:906	porosity	899:906	porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis	899:996	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	6	11	contain	possessed	889:897	arg1	they					884:887	they	884:887	they	884:887	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	0	12	link	Crosslinked	0:10	arg1	nanofibers-gelatine					47:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine biocomposite scaffolds for tissue regeneration.					
32791272	3	13	with	properties	462:471	arg1	degradation					491:501	slow in-vivo degradation	478:501	slow in-vivo degradation	478:501	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	1	14	theme	extracellular	203:215	arg1	matrix					217:222	the extracellular matrix	199:222	the extracellular matrix	199:222	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	5	15	theme	biocomposite	779:790	arg1	aerogels					792:799	the biocomposite aerogels	775:799	the biocomposite aerogels	775:799	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	9	16	theme	crosslinked	1372:1382	arg1	formulation					1384:1394	the optimal crosslinked formulation	1360:1394	the optimal crosslinked formulation with tuned enzymatic degradation	1360:1427	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	8	17	theme	compressive	1219:1229	arg1	strength					1231:1238	the highest compressive strength	1207:1238	the highest compressive strength of 61.35 kPa	1207:1251	The formulation of crosslinked CNF-GEL 90:10 offered the highest compressive strength of 61.35 kPa.					
32791272	0	18	theme	porous	12:17	arg1	nanofibers-gelatine					47:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine biocomposite scaffolds for tissue regeneration.					
32791272	6	19	theme	%	914:914	arg1	porosity					899:906	porosity	899:906	porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis	899:996	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	5	20	theme	chemical	664:671	arg1	crosslinker					673:683	a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels	662:799	a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels	662:799	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	9	21	theme	optimal	1364:1370	arg1	formulation					1384:1394	the optimal crosslinked formulation	1360:1394	the optimal crosslinked formulation with tuned enzymatic degradation	1360:1427	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	3	22	theme	slow	478:481	arg1	degradation					491:501	slow in-vivo degradation	478:501	slow in-vivo degradation	478:501	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	0	23	theme	Crosslinked	0:10	arg1	nanofibers-gelatine					47:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine biocomposite scaffolds for tissue regeneration.					
32791272	4	24	theme	biocomposite	584:595	arg1	aerogels					597:604	biocomposite aerogels	584:604	biocomposite aerogels	584:604	To fine tune their properties, CNF, and gelatine (CNF-GEL) were blended to form biocomposite aerogels.					
32791272	1	25	with	polymer	155:161	arg1	properties					177:186	chemical properties	168:186	chemical properties similar to the extracellular matrix	168:222	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	9	26	theme	in-vitro	1258:1265	arg1	studies					1279:1285	The in-vitro and in-vivo studies	1254:1285	The in-vitro and in-vivo studies	1254:1285	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	8	27	theme	CNF-GEL	1185:1191	arg1	formulation					1158:1168	The formulation	1154:1168	The formulation of crosslinked CNF-GEL 90:10	1154:1197	The formulation of crosslinked CNF-GEL 90:10 offered the highest compressive strength of 61.35 kPa.					
32791272	3	28	theme	in-vivo	483:489	arg1	degradation					491:501	slow in-vivo degradation	478:501	slow in-vivo degradation	478:501	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	0	29	theme	cellulose	37:45	arg1	nanofibers-gelatine					47:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine biocomposite scaffolds for tissue regeneration.					
32791272	9	30	theme	tuned	1401:1405	arg1	degradation					1417:1427	tuned enzymatic degradation	1401:1427	tuned enzymatic degradation	1401:1427	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	10	31	theme	optimal	1478:1484	arg1	scaffold					1486:1493	the optimal scaffold	1474:1493	the optimal scaffold	1474:1493	Antimicrobial property was also achieved in the optimal scaffold by incorporating curcumin as an antimicrobial agent.					
32791272	2	32	theme	scaffold	344:351	arg1	fabrication					353:363	3D scaffold fabrication	341:363	3D scaffold fabrication	341:363	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	7	33	link	uncrosslinked	1131:1143	arg1	samples					1145:1151	uncrosslinked samples	1131:1151	uncrosslinked samples	1131:1151	The presence of EPH improved the chemical interaction between CNF and gelatine, hence enhanced the compressive strength compared to uncrosslinked samples.					
32791272	3	34	theme	surface	424:430	arg1	area					432:435	high surface area	419:435	high surface area	419:435	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	6	35	theme	morphological	975:987	arg1	analysis					989:996	the morphological analysis	971:996	the morphological analysis	971:996	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	4	36	theme	fine	507:510	arg1	tune					512:515	fine tune	507:515	fine tune	507:515	To fine tune their properties, CNF, and gelatine (CNF-GEL) were blended to form biocomposite aerogels.					
32791272	2	37	theme	3D	341:342	arg1	fabrication					353:363	3D scaffold fabrication	341:363	3D scaffold fabrication	341:363	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	5	38	theme	biological	750:759	arg1	properties					761:770	the physiochemical, mechanical, and biological properties	714:770	properties	761:770	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	2	39	contain	has	237:239	arg1	it					234:235	it	234:235	it	234:235	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	2	39	contain	has	237:239	arg2	properties					257:266	poor mechanical properties	241:266	poor mechanical properties	241:266	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	6	40	with	porosity	899:906	arg1	size					930:933	the pore size	921:933	the pore size	921:933	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	3	41	with	area	432:435	arg1	degradation					491:501	slow in-vivo degradation	478:501	slow in-vivo degradation	478:501	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	8	42	theme	crosslinked	1173:1183	arg1	CNF-GEL					1185:1191	crosslinked CNF-GEL 90:10	1173:1197	crosslinked CNF-GEL 90:10	1173:1197	The formulation of crosslinked CNF-GEL 90:10 offered the highest compressive strength of 61.35 kPa.					
32791272	3	43	theme	high	419:422	arg1	area					432:435	high surface area	419:435	high surface area	419:435	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	7	44	theme	chemical	1032:1039	arg1	interaction					1041:1051	the chemical interaction	1028:1051	the chemical interaction between CNF and gelatine, hence enhanced the compressive strength compared to uncrosslinked samples	1028:1151	The presence of EPH improved the chemical interaction between CNF and gelatine, hence enhanced the compressive strength compared to uncrosslinked samples.					
32791272	3	45	theme	excellent	441:449	arg1	properties					462:471	excellent mechanical properties	441:471	excellent mechanical properties	441:471	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	5	46	from	effect	704:709	arg1	properties					761:770	the physiochemical, mechanical, and biological properties	714:770	properties	761:770	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	5	46	from	effect	704:709	arg1	mechanical					734:743	mechanical	734:743	mechanical	734:743	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	9	47	with	formulation	1384:1394	arg1	degradation					1417:1427	tuned enzymatic degradation	1401:1427	tuned enzymatic degradation	1401:1427	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	9	48	theme	adequate	1294:1301	arg1	cytocompatibility					1303:1319	adequate cytocompatibility	1294:1319	adequate cytocompatibility	1294:1319	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	3	49	with	biocompatibility	401:416	arg1	degradation					491:501	slow in-vivo degradation	478:501	slow in-vivo degradation	478:501	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	8	50	link	crosslinked	1173:1183	arg1	CNF-GEL					1185:1191	crosslinked CNF-GEL 90:10	1173:1197	crosslinked CNF-GEL 90:10	1173:1197	The formulation of crosslinked CNF-GEL 90:10 offered the highest compressive strength of 61.35 kPa.					
32791272	1	51	theme	natural	147:153	arg1	Gelatine					115:122	Gelatine	115:122	Gelatine	115:122	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	1	51	theme	natural	147:153	arg1	polymer					155:161	a biocompatible and natural polymer	127:161	a biocompatible and natural polymer with chemical properties similar to the extracellular matrix	127:222	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	3	52	theme	mechanical	451:460	arg1	properties					462:471	excellent mechanical properties	441:471	excellent mechanical properties	441:471	Cellulose nanofibrous (CNF) offers biocompatibility, high surface area and excellent mechanical properties with slow in-vivo degradation.					
32791272	2	53	theme	poor	241:244	arg1	properties					257:266	poor mechanical properties	241:266	poor mechanical properties	241:266	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	2	54	theme	mechanical	246:255	arg1	properties					257:266	poor mechanical properties	241:266	poor mechanical properties	241:266	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	9	55	theme	cell	1341:1344	arg1	attachment					1346:1355	cell attachment	1341:1355	cell attachment	1341:1355	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	5	56	dep	CNF-GEL	651:657	arg1	both					801:804	both	801:804	both	801:804	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	9	57	theme	enzymatic	1407:1415	arg1	degradation					1417:1427	tuned enzymatic degradation	1401:1427	tuned enzymatic degradation	1401:1427	The in-vitro and in-vivo studies showed adequate cytocompatibility, cell viability and cell attachment in the optimal crosslinked formulation with tuned enzymatic degradation.					
32791272	1	58	theme	biocompatible	129:141	arg1	Gelatine					115:122	Gelatine	115:122	Gelatine	115:122	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	1	58	theme	biocompatible	129:141	arg1	polymer					155:161	a biocompatible and natural polymer	127:161	a biocompatible and natural polymer with chemical properties similar to the extracellular matrix	127:222	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	6	59	theme	aerogels	874:881	arg1	composition					846:856	the composition	842:856	the composition of the prepared aerogels	842:881	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	1	60	theme	chemical	168:175	arg1	properties					177:186	chemical properties	168:186	chemical properties similar to the extracellular matrix	168:222	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
32791272	5	61	theme	aerogels	792:799	arg1	mechanical					734:743	mechanical	734:743	mechanical	734:743	Epichlorohydrin (EPH) was incorporated into CNF-GEL as a chemical crosslinker to investigate its effect on the physiochemical, mechanical, and biological properties of the biocomposite aerogels both in-vitro and in-vivo.					
32791272	6	62	theme	prepared	865:872	arg1	aerogels					874:881	the prepared aerogels	861:881	the prepared aerogels	861:881	Regardless of the composition of the prepared aerogels, they possessed porosity of >90% with the pore size of 7-135 μm, which was confirmed in the morphological analysis.					
32791272	2	63	theme	enzymatic	285:293	arg1	biodegradation					295:308	enzymatic biodegradation	285:308	enzymatic biodegradation that limits its application in 3D scaffold fabrication	285:363	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	7	64	theme	EPH	1015:1017	arg1	presence					1003:1010	The presence	999:1010	The presence of EPH	999:1017	The presence of EPH improved the chemical interaction between CNF and gelatine, hence enhanced the compressive strength compared to uncrosslinked samples.					
32791272	2	65	from	application	326:336	arg1	fabrication					353:363	3D scaffold fabrication	341:363	3D scaffold fabrication	341:363	However, it has poor mechanical properties and sensitive to enzymatic biodegradation that limits its application in 3D scaffold fabrication.					
32791272	0	66	theme	tissue	94:99	arg1	regeneration					101:112	tissue regeneration	94:112	tissue regeneration	94:112	Crosslinked porous three-dimensional cellulose nanofibers-gelatine biocomposite scaffolds for tissue regeneration.					
32791272	0	67	theme	three-dimensional	19:35	arg1	nanofibers-gelatine					47:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine	0:65	Crosslinked porous three-dimensional cellulose nanofibers-gelatine biocomposite scaffolds for tissue regeneration.					
32791272	10	68	theme	Antimicrobial	1430:1442	arg1	property					1444:1451	Antimicrobial property	1430:1451	Antimicrobial property	1430:1451	Antimicrobial property was also achieved in the optimal scaffold by incorporating curcumin as an antimicrobial agent.					
32791272	1	69	theme	similar	188:194	arg1	properties					177:186	chemical properties	168:186	chemical properties similar to the extracellular matrix	168:222	Gelatine is a biocompatible and natural polymer with chemical properties similar to the extracellular matrix.					
34425709	9	0	theme	C8:0	1599:1602	arg1	chain					1609:1613	a C8:0 acyl chain	1597:1613	a C8:0 acyl chain	1597:1613	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	9	1	theme	chain	1609:1613	arg1	incorporation					1580:1592	incorporation	1580:1592	incorporation of a C8:0 acyl chain under cold conditions	1580:1635	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	11	2	theme	other	1993:1997	arg1	stresses					1999:2006	other stresses	1993:2006	other stresses	1993:2006	IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses.					
34425709	8	3	dep	position	1442:1449	arg1	upregulated					1493:1503	upregulated	1493:1503	was highly upregulated under cold conditions	1482:1525	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	3	dep	position	1442:1449	arg1	increases					1538:1546	increases	1538:1546	likely increases membrane fluidity	1531:1564	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	9	4	theme	cold	1621:1624	arg1	conditions					1626:1635	cold conditions	1621:1635	cold conditions	1621:1635	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	13	5	theme	A.	2193:2194	arg1	component					2246:2254	key component	2242:2254	key component of the bacterial outer membrane	2242:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	5	theme	A.	2193:2194	arg1	feature					2230:2236	a major structural feature	2211:2236	a major structural feature	2211:2236	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	5	theme	A.	2193:2194	arg1	LOS					2206:2208	A. baumannii LOS	2193:2208	A. baumannii LOS	2193:2208	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	4	6	theme	bacteria	684:691	arg1	case					662:665	the case	658:665	the case of Gram-negative bacteria	658:691	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	10	7	theme	LpxS	1709:1712	arg1	orthologs					1714:1722	LpxS orthologs	1709:1722	LpxS orthologs	1709:1722	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	14	8	theme	LPS	2414:2416	arg1	alterations					2418:2428	LOS or LPS alterations	2407:2428	LOS or LPS alterations under these conditions	2407:2451	Although much is known regarding how glycerophospholipids are altered during temperature shifts, our understanding of LOS or LPS alterations under these conditions is lacking.					
34425709	11	9	from	shifts	1968:1973	arg1	temperature					1978:1988	temperature	1978:1988	temperature	1978:1988	IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses.					
34425709	11	9	from	shifts	1968:1973	arg1	stresses					1999:2006	other stresses	1993:2006	other stresses	1993:2006	IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses.					
34425709	10	10	theme	Acinetobacter	1745:1757	arg1	species					1759:1765	several Acinetobacter species	1737:1765	several Acinetobacter species	1737:1765	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	5	11	theme	lipid	890:894	arg1	domain					898:903	the highly conserved lipid A domain	869:903	the highly conserved lipid A domain	869:903	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	5	12	theme	cell	853:856	arg1	surface					858:864	the cell surface	849:864	the cell surface	849:864	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	12	13	theme	fluidity	2091:2098	arg1	maintenance					2068:2078	maintenance	2068:2078	maintenance of optimal fluidity and membrane permeability	2068:2124	This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability.					
34425709	3	14	theme	growth	626:631	arg1	temperature					633:643	growth temperature	626:643	growth temperature	626:643	It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature.					
34425709	13	15	theme	outer	2273:2277	arg1	membrane					2279:2286	the bacterial outer membrane	2259:2286	the bacterial outer membrane	2259:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	9	16	theme	membrane	1678:1685	arg1	barrier					1700:1706	the outer membrane permeability barrier	1668:1706	the outer membrane permeability barrier	1668:1706	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	8	17	theme	fatty	1421:1425	arg1	acid					1427:1430	a C12:0 fatty acid	1413:1430	a C12:0 fatty acid	1413:1430	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	11	18	theme	membranes	1931:1939	arg1	composition					1905:1915	the composition	1901:1915	the composition of biological membranes	1901:1939	IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses.					
34425709	9	19	theme	barrier	1700:1706	arg1	effectiveness					1651:1663	the effectiveness	1647:1663	the effectiveness of the outer membrane permeability barrier	1647:1706	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	1	20	from	domains	189:195	arg1	cells					174:178	cells	174:178	cells from all domains of life	174:203	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	1	21	theme	optimal	147:153	arg1	dynamics					164:171	optimal membrane dynamics	147:171	optimal membrane dynamics	147:171	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	13	22	theme	key	2242:2244	arg1	LOS					2206:2208	A. baumannii LOS	2193:2208	A. baumannii LOS	2193:2208	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	22	theme	key	2242:2244	arg1	component					2246:2254	key component	2242:2254	key component of the bacterial outer membrane	2242:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	7	23	theme	care	1224:1227	arg1	settings					1229:1236	health care settings	1217:1236	health care settings	1217:1236	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	10	24	theme	common	1787:1792	arg1	mechanism					1794:1802	a common mechanism	1785:1802	a common mechanism for adaptation to cold temperatures in these organisms	1785:1857	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	12	25	theme	homeoviscous	2032:2043	arg1	adaptation					2045:2054	homeoviscous adaptation	2032:2054	homeoviscous adaptation	2032:2054	This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability.					
34425709	13	26	theme	structural	2219:2228	arg1	feature					2230:2236	a major structural feature	2211:2236	a major structural feature	2211:2236	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	26	theme	structural	2219:2228	arg1	LOS					2206:2208	A. baumannii LOS	2193:2208	A. baumannii LOS	2193:2208	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	6	27	theme	novel	985:989	arg1	acyltransferase					991:1005	a novel acyltransferase	983:1005	a novel acyltransferase	983:1005	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	8	28	theme	lipid	1454:1458	arg1	Expression					1463:1472	lipid A. Expression	1454:1472	lipid A. Expression of LpxS	1454:1480	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	0	29	theme	Structure	105:113	arg1	Alteration					71:80	Alteration	71:80	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.	0:133	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	15	30	theme	cold	2487:2490	arg1	mechanism					2503:2511	a cold adaptation mechanism	2485:2511	a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen	2485:2580	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	2	31	theme	lipid	409:413	arg1	bilayer					415:421	the lipid bilayer	405:421	the lipid bilayer	405:421	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	0	32	theme	Cold	122:125	arg1	Stress					127:132	Cold Stress	122:132	Cold Stress	122:132	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	6	33	theme	lipid	1054:1058	arg1	domain					1062:1067	the lipid A domain	1050:1067	the lipid A domain of Acinetobacter baumannii	1050:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	6	33	theme	lipid	1054:1058	arg1	baumannii					1086:1094	Acinetobacter baumannii	1072:1094	Acinetobacter baumannii	1072:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	8	34	theme	LpxS	1477:1480	arg1	Expression					1463:1472	lipid A. Expression	1454:1472	lipid A. Expression of LpxS	1454:1480	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	0	35	theme	Homeoviscous	0:11	arg1	Adaptation					13:22	Homeoviscous Adaptation	0:22	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.	0:133	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	8	36	theme	known	1358:1362	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	36	theme	known	1358:1362	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	3	37	from	changes	615:621	arg1	temperature					633:643	growth temperature	626:643	growth temperature	626:643	It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature.					
34425709	2	38	theme	protein	428:434	arg1	function					436:443	protein function	428:443	protein function	428:443	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	3	39	with	bacteria	601:608	arg1	changes					615:621	changes	615:621	changes in growth temperature	615:643	It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature.					
34425709	5	40	theme	membrane	953:960	arg1	leaflet					932:938	the outer leaflet	922:938	the outer leaflet of the outer membrane	922:960	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	1	41	theme	membrane	155:162	arg1	dynamics					164:171	optimal membrane dynamics	147:171	optimal membrane dynamics	147:171	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	8	42	theme	acyl	1374:1377	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	42	theme	acyl	1374:1377	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	0	43	theme	Acinetobacter	31:43	arg1	Membrane					61:68	the Acinetobacter baumannii Outer Membrane	27:68	the Acinetobacter baumannii Outer Membrane	27:68	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	14	44	theme	LOS	2407:2409	arg1	alterations					2418:2428	LOS or LPS alterations	2407:2428	LOS or LPS alterations under these conditions	2407:2451	Although much is known regarding how glycerophospholipids are altered during temperature shifts, our understanding of LOS or LPS alterations under these conditions is lacking.					
34425709	0	45	theme	Outer	55:59	arg1	Membrane					61:68	the Acinetobacter baumannii Outer Membrane	27:68	the Acinetobacter baumannii Outer Membrane	27:68	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	13	46	theme	acid	2177:2180	arg1	content					2182:2188	the fatty acid content	2167:2188	the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane	2167:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	0	47	dep	Acinetobacter	31:43	arg1	baumannii					45:53	baumannii	45:53	baumannii	45:53	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	4	48	theme	temperature	806:816	arg1	shifts					818:823	temperature shifts	806:823	temperature shifts	806:823	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	15	49	theme	multidrug-resistant	2553:2571	arg1	pathogen					2573:2580	a highly adaptable and multidrug-resistant pathogen	2530:2580	a highly adaptable and multidrug-resistant pathogen	2530:2580	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	15	49	theme	multidrug-resistant	2553:2571	arg1	baumannii					2519:2527	A. baumannii	2516:2527	A. baumannii	2516:2527	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	1	50	theme	various	223:229	arg1	signals					245:251	various environmental signals	223:251	various environmental signals	223:251	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	8	51	theme	membrane	1548:1555	arg1	fluidity					1557:1564	membrane fluidity	1548:1564	membrane fluidity	1548:1564	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	3	52	theme	glycerophospholipid	512:530	arg1	content					532:538	glycerophospholipid content	512:538	glycerophospholipid content	512:538	It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature.					
34425709	15	53	theme	adaptable	2539:2547	arg1	pathogen					2573:2580	a highly adaptable and multidrug-resistant pathogen	2530:2580	a highly adaptable and multidrug-resistant pathogen	2530:2580	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	15	53	theme	adaptable	2539:2547	arg1	baumannii					2519:2527	A. baumannii	2516:2527	A. baumannii	2516:2527	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	6	54	theme	Acinetobacter	1072:1084	arg1	baumannii					1086:1094	Acinetobacter baumannii	1072:1094	Acinetobacter baumannii	1072:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	14	55	theme	temperature	2366:2376	arg1	shifts					2378:2383	temperature shifts	2366:2383	temperature shifts	2366:2383	Although much is known regarding how glycerophospholipids are altered during temperature shifts, our understanding of LOS or LPS alterations under these conditions is lacking.					
34425709	2	56	theme	membrane	370:377	arg1	fluidity					379:386	membrane fluidity	370:386	membrane fluidity	370:386	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	10	57	from	temperatures	1827:1838	arg1	organisms					1849:1857	these organisms	1843:1857	these organisms	1843:1857	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	9	58	theme	acyl	1604:1607	arg1	chain					1609:1613	a C8:0 acyl chain	1597:1613	a C8:0 acyl chain	1597:1613	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	6	59	theme	domain	1062:1067	arg1	synthesis					1037:1045	the synthesis	1033:1045	the synthesis of the lipid A domain of Acinetobacter baumannii	1033:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	3	60	theme	Gram-positive	587:599	arg1	bacteria					601:608	both Gram-negative and Gram-positive bacteria	564:608	both Gram-negative and Gram-positive bacteria with changes in growth temperature	564:643	It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature.					
34425709	15	61	from	mechanism	2503:2511	arg1	pathogen					2573:2580	a highly adaptable and multidrug-resistant pathogen	2530:2580	a highly adaptable and multidrug-resistant pathogen	2530:2580	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	15	61	from	mechanism	2503:2511	arg1	baumannii					2519:2527	A. baumannii	2516:2527	A. baumannii	2516:2527	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	13	62	theme	membrane	2279:2286	arg1	LOS					2206:2208	A. baumannii LOS	2193:2208	A. baumannii LOS	2193:2208	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	62	theme	membrane	2279:2286	arg1	feature					2230:2236	a major structural feature	2211:2236	a major structural feature	2211:2236	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	62	theme	membrane	2279:2286	arg1	component					2246:2254	key component	2242:2254	key component of the bacterial outer membrane	2242:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	7	63	theme	significant	1115:1125	arg1	difficult					1193:1201	difficult	1193:1201	difficult	1193:1201	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	7	63	theme	significant	1115:1125	arg1	A.					1097:1098	A.	1097:1098	A.	1097:1098	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	7	63	theme	significant	1115:1125	arg1	pathogen					1163:1170	a significant, multidrug-resistant, opportunistic pathogen	1113:1170	pathogen	1163:1170	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	14	64	theme	alterations	2418:2428	arg1	understanding					2390:2402	our understanding	2386:2402	our understanding of LOS or LPS alterations under these conditions	2386:2451	Although much is known regarding how glycerophospholipids are altered during temperature shifts, our understanding of LOS or LPS alterations under these conditions is lacking.					
34425709	4	65	theme	Gram-negative	670:682	arg1	bacteria					684:691	Gram-negative bacteria	670:691	Gram-negative bacteria	670:691	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	2	66	theme	lipid	324:328	arg1	composition					330:340	the lipid composition	320:340	the lipid composition	320:340	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	5	67	theme	conserved	880:888	arg1	domain					898:903	the highly conserved lipid A domain	869:903	the highly conserved lipid A domain	869:903	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	12	68	theme	permeability	2113:2124	arg1	maintenance					2068:2078	maintenance	2068:2078	maintenance of optimal fluidity and membrane permeability	2068:2124	This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability.					
34425709	3	69	theme	Gram-negative	569:581	arg1	bacteria					601:608	both Gram-negative and Gram-positive bacteria	564:608	both Gram-negative and Gram-positive bacteria with changes in growth temperature	564:643	It is well documented, for example, that glycerophospholipid content varies substantially in both Gram-negative and Gram-positive bacteria with changes in growth temperature.					
34425709	1	70	theme	life	200:203	arg1	domains					189:195	all domains	185:195	all domains of life	185:203	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	1	70	theme	life	200:203	arg1	life					200:203	life	200:203	life	200:203	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	8	71	theme	C12:0	1415:1419	arg1	acid					1427:1430	a C12:0 fatty acid	1413:1430	a C12:0 fatty acid	1413:1430	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	13	72	theme	bacterial	2263:2271	arg1	membrane					2279:2286	the bacterial outer membrane	2259:2286	the bacterial outer membrane	2259:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	9	73	theme	outer	1672:1676	arg1	barrier					1700:1706	the outer membrane permeability barrier	1668:1706	the outer membrane permeability barrier	1668:1706	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	12	74	theme	optimal	2083:2089	arg1	fluidity					2091:2098	optimal fluidity	2083:2098	optimal fluidity	2083:2098	This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability.					
34425709	11	75	theme	biological	1920:1929	arg1	membranes					1931:1939	biological membranes	1920:1939	biological membranes	1920:1939	IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses.					
34425709	13	76	dep	A.	2193:2194	arg1	baumannii					2196:2204	baumannii	2196:2204	baumannii	2196:2204	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	9	77	theme	permeability	1687:1698	arg1	barrier					1700:1706	the outer membrane permeability barrier	1668:1706	the outer membrane permeability barrier	1668:1706	Furthermore, incorporation of a C8:0 acyl chain under cold conditions increased the effectiveness of the outer membrane permeability barrier.					
34425709	4	78	theme	structural	723:732	arg1	changes					734:740	structural changes	723:740	structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS)	723:797	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	5	79	theme	outer	926:930	arg1	leaflet					932:938	the outer leaflet	922:938	the outer leaflet of the outer membrane	922:960	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	10	80	theme	several	1737:1743	arg1	species					1759:1765	several Acinetobacter species	1737:1765	several Acinetobacter species	1737:1765	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	7	81	theme	health	1217:1222	arg1	settings					1229:1236	health care settings	1217:1236	health care settings	1217:1236	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	5	82	theme	A	896:896	arg1	domain					898:903	the highly conserved lipid A domain	869:903	the highly conserved lipid A domain	869:903	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	10	83	located	found	1728:1732	arg2	orthologs					1714:1722	LpxS orthologs	1709:1722	LpxS orthologs	1709:1722	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	10	83	located	found	1728:1732	arg1	species					1759:1765	several Acinetobacter species	1737:1765	several Acinetobacter species	1737:1765	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	0	84	theme	Lipooligosaccharide	85:103	arg1	Structure					105:113	Lipooligosaccharide Structure	85:113	Lipooligosaccharide Structure	85:113	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	13	85	theme	major	2213:2217	arg1	feature					2230:2236	a major structural feature	2211:2236	a major structural feature	2211:2236	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	13	85	theme	major	2213:2217	arg1	LOS					2206:2208	A. baumannii LOS	2193:2208	A. baumannii LOS	2193:2208	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	8	86	theme	A.	1460:1461	arg1	Expression					1463:1472	lipid A. Expression	1454:1472	lipid A. Expression of LpxS	1454:1480	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	87	theme	octanoate	1316:1324	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	87	theme	octanoate	1316:1324	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	88	theme	C8:0	1327:1330	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	88	theme	C8:0	1327:1330	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	15	89	theme	adaptation	2492:2501	arg1	mechanism					2503:2511	a cold adaptation mechanism	2485:2511	a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen	2485:2580	Our work identifies a cold adaptation mechanism in A. baumannii, a highly adaptable and multidrug-resistant pathogen.					
34425709	6	90	theme	A	1060:1060	arg1	domain					1062:1067	the lipid A domain	1050:1067	the lipid A domain of Acinetobacter baumannii	1050:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	6	90	theme	A	1060:1060	arg1	baumannii					1086:1094	Acinetobacter baumannii	1072:1094	Acinetobacter baumannii	1072:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	7	91	dep	significant	1115:1125	arg1	multidrug-resistant					1128:1146	multidrug-resistant	1128:1146	multidrug-resistant	1128:1146	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	8	92	theme	fatty	1333:1337	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	92	theme	fatty	1333:1337	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	2	93	theme	diverse	451:457	arg1	conditions					459:468	diverse conditions	451:468	diverse conditions	451:468	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	1	94	theme	homeoviscous	281:292	arg1	adaptation					294:303	homeoviscous adaptation	281:303	homeoviscous adaptation	281:303	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	5	95	theme	outer	947:951	arg1	membrane					953:960	the outer membrane	943:960	the outer membrane	943:960	LPS/LOS is anchored at the cell surface by the highly conserved lipid A domain and localized in the outer leaflet of the outer membrane.					
34425709	8	96	theme	cold	1511:1514	arg1	conditions					1516:1525	cold conditions	1511:1525	cold conditions	1511:1525	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	97	theme	shortest	1349:1356	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	97	theme	shortest	1349:1356	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	13	98	theme	LOS	2206:2208	arg1	content					2182:2188	the fatty acid content	2167:2188	the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane	2167:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	7	99	theme	diverse	1278:1284	arg1	conditions					1286:1295	diverse conditions	1278:1295	diverse conditions	1278:1295	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	11	100	theme	cellular	1883:1890	arg1	fitness					1892:1898	cellular fitness	1883:1898	cellular fitness	1883:1898	IMPORTANCE To maintain cellular fitness, the composition of biological membranes must change in response to shifts in temperature or other stresses.					
34425709	10	101	theme	cold	1822:1825	arg1	temperatures					1827:1838	cold temperatures	1822:1838	cold temperatures in these organisms	1822:1857	LpxS orthologs are found in several Acinetobacter species and may represent a common mechanism for adaptation to cold temperatures in these organisms.					
34425709	8	102	theme	secondary	1364:1372	arg1	chain					1379:1383	the shortest known secondary acyl chain	1345:1383	the shortest known secondary acyl chain reported to date	1345:1400	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	8	102	theme	secondary	1364:1372	arg1	acid					1339:1342	an octanoate (C8:0) fatty acid	1313:1342	an octanoate (C8:0) fatty acid	1313:1342	LpxS transfers an octanoate (C8:0) fatty acid, the shortest known secondary acyl chain reported to date, replacing a C12:0 fatty acid at the 2' position of lipid A. Expression of LpxS was highly upregulated under cold conditions and likely increases membrane fluidity.					
34425709	2	103	theme	bilayer	415:421	arg1	permeability					389:400	permeability	389:400	permeability of the lipid bilayer	389:421	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	2	103	theme	bilayer	415:421	arg1	fluidity					379:386	membrane fluidity	370:386	membrane fluidity	370:386	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	2	103	theme	bilayer	415:421	arg1	function					436:443	protein function	428:443	protein function	428:443	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	0	104	theme	Membrane	61:68	arg1	Adaptation					13:22	Homeoviscous Adaptation	0:22	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.	0:133	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	13	105	theme	fatty	2171:2175	arg1	content					2182:2188	the fatty acid content	2167:2188	the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane	2167:2286	Here, we describe an enzyme that alters the fatty acid content of A. baumannii LOS, a major structural feature and key component of the bacterial outer membrane.					
34425709	2	106	theme	composition	330:340	arg1	Alteration					306:315	Alteration	306:315	Alteration of the lipid composition	306:340	Alteration of the lipid composition is critical for maintaining membrane fluidity, permeability of the lipid bilayer, and protein function under diverse conditions.					
34425709	4	107	from	changes	734:740	arg1	LOS					794:796	LOS	794:796	LOS	794:796	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	4	107	from	changes	734:740	arg1	LPS					765:767	LPS	765:767	LPS	765:767	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	4	107	from	changes	734:740	arg1	lipooligosaccharide					773:791	lipooligosaccharide	773:791	lipooligosaccharide (LOS)	773:797	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	4	107	from	changes	734:740	arg1	lipopolysaccharide					745:762	lipopolysaccharide	745:762	lipopolysaccharide (LPS)	745:768	However, in the case of Gram-negative bacteria, far less is known concerning structural changes in lipopolysaccharide (LPS) or lipooligosaccharide (LOS) during temperature shifts.					
34425709	1	108	theme	environmental	231:243	arg1	signals					245:251	various environmental signals	223:251	various environmental signals	223:251	To maintain optimal membrane dynamics, cells from all domains of life must acclimate to various environmental signals in a process referred to as homeoviscous adaptation.					
34425709	12	109	theme	membrane	2104:2111	arg1	permeability					2113:2124	membrane permeability	2104:2124	membrane permeability	2104:2124	This process, known as homeoviscous adaptation, allows for maintenance of optimal fluidity and membrane permeability.					
34425709	0	110	dep	Adaptation	13:22	arg1	Alteration					71:80	Alteration	71:80	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.	0:133	Homeoviscous Adaptation of the Acinetobacter baumannii Outer Membrane: Alteration of Lipooligosaccharide Structure during Cold Stress.					
34425709	6	111	theme	baumannii	1086:1094	arg1	domain					1062:1067	the lipid A domain	1050:1067	the lipid A domain of Acinetobacter baumannii	1050:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	6	111	theme	baumannii	1086:1094	arg1	baumannii					1086:1094	Acinetobacter baumannii	1072:1094	Acinetobacter baumannii	1072:1094	Here, we identified a novel acyltransferase, termed LpxS, involved in the synthesis of the lipid A domain of Acinetobacter baumannii.					
34425709	7	112	theme	opportunistic	1149:1161	arg1	difficult					1193:1201	difficult	1193:1201	difficult	1193:1201	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	7	112	theme	opportunistic	1149:1161	arg1	A.					1097:1098	A.	1097:1098	A.	1097:1098	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
34425709	7	112	theme	opportunistic	1149:1161	arg1	pathogen					1163:1170	a significant, multidrug-resistant, opportunistic pathogen	1113:1170	pathogen	1163:1170	A. baumannii is a significant, multidrug-resistant, opportunistic pathogen that is particularly difficult to clear from health care settings because of its ability to survive under diverse conditions.					
32120998	0	0	theme	Potential	87:95	arg1	Candidates					97:106	Potential Candidates	87:106	Potential Candidates for Fast Bone Regeneration Applications	87:146	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	6	1	theme	injection	1140:1148	arg1	force					1150:1154	the maximum injection force	1128:1154	the maximum injection force measured through 19 G needle (17.191 ± 2.296N)	1128:1201	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	1	theme	injection	1140:1148	arg1	convenient					1207:1216	convenient	1207:1216	convenient	1207:1216	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	8	2	theme	histology	1476:1484	arg1	analyses					1486:1493	histology analyses	1476:1493	histology analyses	1476:1493	Hydrogels induced microvascularity in CAM assay this was confirmed by histology analyses.					
32120998	7	3	dep	in	1387:1388	arg1	situ					1390:1393	situ	1390:1393	situ	1390:1393	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	6	4	theme	maximum	1132:1138	arg1	force					1150:1154	the maximum injection force	1128:1154	the maximum injection force measured through 19 G needle (17.191 ± 2.296N)	1128:1201	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	4	theme	maximum	1132:1138	arg1	convenient					1207:1216	convenient	1207:1216	convenient	1207:1216	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	9	5	theme	Hep	1534:1536	arg1	concentration					1517:1529	lower concentration	1511:1529	lower concentration of Hep	1511:1536	Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response.					
32120998	4	6	theme	Hep	608:610	arg1	concentrations					612:625	varying Hep concentrations	600:625	varying Hep concentrations	600:625	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	10	7	dep	regeneration	1693:1704	arg1	applications					1724:1735	applications	1724:1735	applications	1724:1735	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	0	8	dep	Stimulate	63:71	arg1	Candidates					97:106	Potential Candidates	87:106	Potential Candidates for Fast Bone Regeneration Applications	87:146	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	5	9	theme	biological	967:976	arg1	injectability					907:919	injectability	907:919	injectability	907:919	Hydrogels were characterised by performing injectability, gelation, rheology, morphology, chemical and biological analyses.					
32120998	5	9	theme	biological	967:976	arg1	analyses					978:985	chemical and biological analyses	954:985	chemical and biological analyses	954:985	Hydrogels were characterised by performing injectability, gelation, rheology, morphology, chemical and biological analyses.					
32120998	7	10	theme	ex-ovo	1281:1286	arg1	assay					1325:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	0	11	theme	Bone	117:120	arg1	Applications					135:146	Fast Bone Regeneration Applications	112:146	Fast Bone Regeneration Applications	112:146	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	7	12	theme	CAM	1320:1322	arg1	assay					1325:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	9	13	theme	lower	1511:1515	arg1	concentration					1517:1529	lower concentration	1511:1529	lower concentration of Hep	1511:1536	Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response.					
32120998	4	14	with	formulations	582:593	arg1	concentrations					612:625	varying Hep concentrations	600:625	varying Hep concentrations	600:625	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	0	15	theme	Fast	112:115	arg1	Applications					135:146	Fast Bone Regeneration Applications	112:146	Fast Bone Regeneration Applications	112:146	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	3	16	theme	pro-angiogenic	547:560	arg1	potential					562:570	their pro-angiogenic potential	541:570	their pro-angiogenic potential	541:570	In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential.					
32120998	10	17	theme	potential	1663:1671	arg1	hydrogels					1615:1623	novel injectable hydrogels	1598:1623	novel injectable hydrogels inducing angiogenesis (CS/HA/Hep)	1598:1657	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	10	17	theme	potential	1663:1671	arg1	candidates					1673:1682	potential candidates	1663:1682	potential candidates for bone regeneration and drug delivery applications	1663:1735	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	6	18	from	Gauge	1053:1057	arg1	injection					1035:1043	manual dropwise injection	1019:1043	manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations	1019:1121	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	2	19	theme	bone	359:362	arg1	regeneration					364:375	bone regeneration	359:375	bone regeneration	359:375	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	9	20	with	Hydrogels	1496:1504	arg1	concentration					1517:1529	lower concentration	1511:1529	lower concentration of Hep	1511:1536	Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response.					
32120998	10	21	theme	drug	1710:1713	arg1	delivery					1715:1722	drug delivery	1710:1722	drug delivery	1710:1722	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	6	22	theme	dropwise	1026:1033	arg1	injection					1035:1043	manual dropwise injection	1019:1043	manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations	1019:1121	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	23	used	used	1075:1078	arg2	Gauge					1053:1057	21 Gauge	1050:1057	21 Gauge which is highly used for orthopaedic and dental administrations	1050:1121	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	24	theme	±	1193:1193	arg1	2.296N					1195:1200	17.191 ± 2.296N	1186:1200	17.191 ± 2.296N	1186:1200	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	24	theme	±	1193:1193	arg1	needle					1178:1183	19 G needle	1173:1183	19 G needle (17.191 ± 2.296N)	1173:1201	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	3	25	theme	hydroxyapatite	453:466	arg1	materials					483:491	chitosan (CS) and hydroxyapatite (HA) composite materials	435:491	chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep)	435:517	In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential.					
32120998	3	26	theme	thermosensitive	400:414	arg1	hydrogels					416:424	thermosensitive hydrogels	400:424	thermosensitive hydrogels	400:424	In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential.					
32120998	4	27	theme	Hydrogel	573:580	arg1	formulations					582:593	Hydrogel formulations	573:593	Hydrogel formulations with varying Hep concentrations	573:625	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	8	28	theme	CAM	1444:1446	arg1	assay					1448:1452	CAM assay	1444:1452	CAM assay	1444:1452	Hydrogels induced microvascularity in CAM assay this was confirmed by histology analyses.					
32120998	6	29	theme	17.191	1186:1191	arg1	2.296N					1195:1200	17.191 ± 2.296N	1186:1200	17.191 ± 2.296N	1186:1200	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	29	theme	17.191	1186:1191	arg1	needle					1178:1183	19 G needle	1173:1183	19 G needle (17.191 ± 2.296N)	1173:1201	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	4	30	theme	homogeneous	672:682	arg1	solutions					684:692	these homogeneous solutions	666:692	these homogeneous solutions	666:692	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	4	31	theme	varying	600:606	arg1	concentrations					612:625	varying Hep concentrations	600:625	varying Hep concentrations	600:625	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	0	32	theme	Regeneration	122:133	arg1	Applications					135:146	Fast Bone Regeneration Applications	112:146	Fast Bone Regeneration Applications	112:146	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	2	33	theme	high	343:346	arg1	demand					348:353	high demand	343:353	high demand for bone regeneration	343:375	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	7	34	theme	injectable	1343:1352	arg1	solutions					1354:1362	injectable solutions	1343:1362	injectable solutions	1343:1362	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	0	35	theme	Injectable	39:48	arg1	Hydrogels					50:58	Thermosensitive Injectable Hydrogels	23:58	Thermosensitive Injectable Hydrogels	23:58	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	2	36	contain	have	338:341	arg2	demand					348:353	high demand	343:353	high demand for bone regeneration	343:375	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	2	36	contain	have	338:341	arg1	hydrogels					264:272	In-situ formed injectable hydrogels	238:272	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis	238:336	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	6	37	theme	orthopaedic	1084:1094	arg1	administrations					1107:1121	orthopaedic and dental administrations	1084:1121	orthopaedic and dental administrations	1084:1121	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	4	38	theme	sodium	716:721	arg1	NaHCO3					736:741	NaHCO3	736:741	NaHCO3	736:741	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	4	38	theme	sodium	716:721	arg1	bicarbonate					723:733	sodium bicarbonate	716:733	sodium bicarbonate (NaHCO3)	716:742	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	1	39	theme	bone	232:235	arg1	repair					205:210	repair	205:210	repair	205:210	Biomaterials that promote angiogenesis are required for repair and regeneration of bone.					
32120998	1	39	theme	bone	232:235	arg1	regeneration					216:227	regeneration	216:227	regeneration	216:227	Biomaterials that promote angiogenesis are required for repair and regeneration of bone.					
32120998	0	40	theme	Thermosensitive	23:37	arg1	Hydrogels					50:58	Thermosensitive Injectable Hydrogels	23:58	Thermosensitive Injectable Hydrogels	23:58	In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications.					
32120998	6	41	theme	Hydrogel	988:995	arg1	solutions					997:1005	Hydrogel solutions	988:1005	Hydrogel solutions	988:1005	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	10	42	theme	bone	1688:1691	arg1	regeneration					1693:1704	bone regeneration	1688:1704	bone regeneration	1688:1704	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	2	43	theme	injectable	253:262	arg1	hydrogels					264:272	In-situ formed injectable hydrogels	238:272	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis	238:336	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	3	44	theme	composite	473:481	arg1	materials					483:491	chitosan (CS) and hydroxyapatite (HA) composite materials	435:491	chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep)	435:517	In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential.					
32120998	3	45	theme	chitosan	435:442	arg1	materials					483:491	chitosan (CS) and hydroxyapatite (HA) composite materials	435:491	chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep)	435:517	In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential.					
32120998	6	46	theme	manual	1019:1024	arg1	injection					1035:1043	manual dropwise injection	1019:1043	manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations	1019:1121	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	2	47	theme	formed	246:251	arg1	hydrogels					264:272	In-situ formed injectable hydrogels	238:272	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis	238:336	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	4	48	theme	°C.	749:751	arg1	CS/HA/Hep					764:772	CS/HA/Hep	764:772	CS/HA/Hep	764:772	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	4	48	theme	°C.	749:751	arg1	Solutions					753:761	4 °C. Solutions	747:761	4 °C. Solutions (CS/HA/Hep)	747:773	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	9	49	theme	more	1545:1548	arg1	efficiency					1550:1559	more efficiency	1545:1559	more efficiency	1545:1559	Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response.					
32120998	2	50	theme	In-situ	238:244	arg1	hydrogels					264:272	In-situ formed injectable hydrogels	238:272	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis	238:336	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	6	51	theme	G	1176:1176	arg1	2.296N					1195:1200	17.191 ± 2.296N	1186:1200	17.191 ± 2.296N	1186:1200	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	6	51	theme	G	1176:1176	arg1	needle					1178:1183	19 G needle	1173:1183	19 G needle (17.191 ± 2.296N)	1173:1201	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	7	52	theme	in	1387:1388	arg1	hydrogels					1395:1403	in situ hydrogels	1387:1403	in situ hydrogels	1387:1403	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	4	53	from	surface	839:845	arg1	minutes					855:861	5-10 minutes	850:861	5-10 minutes	850:861	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	5	54	theme	chemical	954:961	arg1	injectability					907:919	injectability	907:919	injectability	907:919	Hydrogels were characterised by performing injectability, gelation, rheology, morphology, chemical and biological analyses.					
32120998	5	54	theme	chemical	954:961	arg1	analyses					978:985	chemical and biological analyses	954:985	chemical and biological analyses	954:985	Hydrogels were characterised by performing injectability, gelation, rheology, morphology, chemical and biological analyses.					
32120998	4	55	theme	sol-gel	644:650	arg1	technique					652:660	sol-gel technique	644:660	sol-gel technique	644:660	Hydrogel formulations with varying Hep concentrations were prepared by sol-gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5-10 minutes.					
32120998	2	56	theme	bioactive	294:302	arg1	agents					304:309	bioactive agents	294:309	bioactive agents	294:309	In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration.					
32120998	7	57	from	solutions	1354:1362	arg1	CAM					1367:1369	CAM	1367:1369	CAM	1367:1369	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	9	58	theme	pro-angiogenic	1564:1577	arg1	response					1579:1586	pro-angiogenic response	1564:1586	pro-angiogenic response	1564:1586	Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response.					
32120998	7	59	theme	membrane	1310:1317	arg1	assay					1325:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	10	60	theme	injectable	1604:1613	arg1	candidates					1673:1682	potential candidates	1663:1682	potential candidates for bone regeneration and drug delivery applications	1663:1735	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	10	60	theme	injectable	1604:1613	arg1	hydrogels					1615:1623	novel injectable hydrogels	1598:1623	novel injectable hydrogels inducing angiogenesis (CS/HA/Hep)	1598:1657	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	6	61	theme	dental	1100:1105	arg1	administrations					1107:1121	orthopaedic and dental administrations	1084:1121	orthopaedic and dental administrations	1084:1121	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	7	62	theme	chick	1288:1292	arg1	assay					1325:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	10	63	theme	novel	1598:1602	arg1	candidates					1673:1682	potential candidates	1663:1682	potential candidates for bone regeneration and drug delivery applications	1663:1735	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	10	63	theme	novel	1598:1602	arg1	hydrogels					1615:1623	novel injectable hydrogels	1598:1623	novel injectable hydrogels inducing angiogenesis (CS/HA/Hep)	1598:1657	Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.					
32120998	6	64	theme	manual	1222:1227	arg1	injections					1229:1238	manual injections	1222:1238	manual injections	1222:1238	Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections.					
32120998	7	65	theme	chorioallantoic	1294:1308	arg1	assay					1325:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	an ex-ovo chick chorioallantoic membrane (CAM) assay	1278:1329	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
32120998	7	66	theme	Angiogenesis	1241:1252	arg1	tests					1254:1258	Angiogenesis tests	1241:1258	Angiogenesis tests	1241:1258	Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels.					
33389269	5	0	dep	disintegrant	801:812	arg1	Lycoat					815:820	Lycoat	815:820	Lycoat	815:820	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	8	1	dep	%	1605:1605	arg1	sucralose					1607:1615	sucralose	1607:1615	2% sucralose	1604:1615	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	2	from	drug	1765:1768	arg1	media					1803:1807	four dissolution media	1786:1807	four dissolution media	1786:1807	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	3	theme	desirable	1668:1676	arg1	properties					1689:1698	desirable mechanical properties	1668:1698	desirable mechanical properties	1668:1698	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	4	theme	mechanical	1678:1687	arg1	properties					1689:1698	desirable mechanical properties	1668:1698	desirable mechanical properties	1668:1698	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	7	5	theme	Differential	1273:1284	arg1	DSC					1308:1310	DSC	1308:1310	DSC	1308:1310	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	5	theme	Differential	1273:1284	arg1	calorimetry					1295:1305	Differential scanning calorimetry	1273:1305	Differential scanning calorimetry (DSC) study	1273:1317	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	6	theme	diffraction	1333:1343	arg1	study					1351:1355	powder X-ray diffraction (XRD) study	1320:1355	powder X-ray diffraction (XRD) study	1320:1355	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	7	theme	powder	1320:1325	arg1	XRD					1346:1348	XRD	1346:1348	XRD	1346:1348	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	7	theme	powder	1320:1325	arg1	diffraction					1333:1343	powder X-ray diffraction	1320:1343	powder X-ray diffraction (XRD) study	1320:1355	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	3	8	theme	polyvinyl	526:534	arg1	alcohol					536:542	polyvinyl alcohol	526:542	polyvinyl alcohol	526:542	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	8	theme	polyvinyl	526:534	arg1	PVA					545:547	PVA	545:547	PVA	545:547	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	6	9	theme	drug	1096:1099	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	9	theme	drug	1096:1099	arg1	content					1101:1107	drug content	1096:1107	drug content	1096:1107	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	8	10	dep	%	1763:1763	arg1	85					1761:1762	85	1761:1762	85	1761:1762	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	6	11	theme	moisture	1157:1164	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	11	theme	moisture	1157:1164	arg1	uptake					1166:1171	moisture uptake	1157:1171	moisture uptake	1157:1171	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	3	12	theme	hydroxypropyl	566:578	arg1	L-HPC					591:595	L-HPC	591:595	L-HPC	591:595	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	12	theme	hydroxypropyl	566:578	arg1	cellulose					580:588	low-substituted hydroxypropyl cellulose	550:588	low-substituted hydroxypropyl cellulose	550:588	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	7	13	from	study	1351:1355	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	5	14	dep	glycerol	859:866	arg1	w/w					875:877	w/w	875:877	w/w	875:877	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	5	14	dep	glycerol	859:866	arg1	%					872:872	2-6%	869:872	2-6%	869:872	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	6	15	theme	optimal	1230:1236	arg1	compositions					1238:1249	the optimal compositions	1226:1249	the optimal compositions of racecadotril ODFs	1226:1270	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	1	16	theme	mg	183:184	arg1	racecadotril					186:197	30 mg racecadotril	180:197	30 mg racecadotril	180:197	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	7	17	from	Fourier	1358:1364	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	18	theme	calorimetry	1295:1305	arg1	study					1313:1317	Differential scanning calorimetry (DSC) study	1273:1317	Differential scanning calorimetry (DSC) study	1273:1317	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	10	19	theme	30	1937:1938	arg1	mg					1940:1941	mg	1940:1941	mg	1940:1941	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	7	20	theme	ODFs	1506:1509	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	6	21	theme	moisture	1139:1146	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	21	theme	moisture	1139:1146	arg1	content					1148:1154	moisture content	1139:1154	moisture content	1139:1154	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	5	22	theme	PU	972:973	arg1	films					975:979	PU films	972:979	PU films	972:979	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	8	23	contain	containing	1549:1558	arg2	%					1562:1562	50%	1560:1562	50% racecadotril	1560:1575	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	23	contain	containing	1549:1558	arg1	ODFs					1544:1547	ODFs	1544:1547	ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide	1544:1652	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	23	contain	containing	1549:1558	arg2	%					1588:1588	7%	1587:1588	7% Lycoat RS720	1587:1601	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	23	contain	containing	1549:1558	arg2	%					1605:1605	2%	1604:1605	2% sucralose	1604:1615	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	23	contain	containing	1549:1558	arg2	dioxide					1646:1652	1% titanium dioxide	1634:1652	1% titanium dioxide	1634:1652	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	23	contain	containing	1549:1558	arg2	%					1619:1619	2%	1618:1619	2% apricot	1618:1627	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	23	contain	containing	1549:1558	arg2	%					1580:1580	38%	1578:1580	38% PVA	1578:1584	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	1	24	theme	present	105:111	arg1	study					113:117	The present study	101:117	The present study	101:117	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	7	25	theme	optimized	1483:1491	arg1	ODFs					1506:1509	optimized racecadotril ODFs	1483:1509	optimized racecadotril ODFs	1483:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	6	26	theme	ODFs	1197:1200	arg1	value					1132:1136	pH value	1129:1136	pH value	1129:1136	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	elongation					1063:1072	percent elongation	1055:1072	percent elongation	1055:1072	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	uptake					1166:1171	moisture uptake	1157:1171	moisture uptake	1157:1171	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	content					1101:1107	drug content	1096:1107	drug content	1096:1107	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	appearance					1025:1034	appearance	1025:1034	appearance	1025:1034	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	weight					1110:1115	weight	1110:1115	weight	1110:1115	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	strength					1045:1052	tensile strength	1037:1052	tensile strength	1037:1052	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	content					1148:1154	moisture content	1139:1154	moisture content	1139:1154	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	time					1090:1093	disintegration time	1075:1093	disintegration time	1075:1093	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	Q5min					1178:1182	Q5min	1178:1182	Q5min	1178:1182	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	thickness					1118:1126	thickness	1118:1126	thickness	1118:1126	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	26	theme	ODFs	1197:1200	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	7	27	from	study	1313:1317	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	1	28	theme	choking	303:309	arg1	risk					295:298	risk	295:298	risk of choking	295:309	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	7	29	dep	infrared	1376:1383	arg1	FTIR					1386:1389	FTIR	1386:1389	FTIR	1386:1389	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	10	30	theme	casting	1994:2000	arg1	method					2002:2007	solvent casting method	1986:2007	solvent casting method	1986:2007	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	0	31	theme	Orodispersible	61:74	arg1	Films					76:80	Racecadotril Orodispersible Films	48:80	Racecadotril Orodispersible Films	48:80	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	7	32	theme	pharmacokinetic	1443:1457	arg1	study					1459:1463	pharmacokinetic study	1443:1463	pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs	1443:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	5	33	dep	plasticizers	845:856	arg1	glycerol					859:866	glycerol	859:866	glycerol	859:866	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	6	34	theme	ODFs	1267:1270	arg1	compositions					1238:1249	the optimal compositions	1226:1249	the optimal compositions of racecadotril ODFs	1226:1270	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	8	35	theme	%	1635:1635	arg1	dioxide					1646:1652	1% titanium dioxide	1634:1652	1% titanium dioxide	1634:1652	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	2	36	theme	drug	359:362	arg1	ODFs					372:375	high drug loading ODFs	354:375	high drug loading ODFs with successful mechanical and physicochemical properties	354:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	8	37	dep	drug	1765:1768	arg1	%					1763:1763	%	1763:1763	%	1763:1763	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	9	38	theme	racecadotril	1844:1855	arg1	ODF					1857:1859	optimized racecadotril ODF	1834:1859	optimized racecadotril ODF	1834:1859	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	7	39	dep	Fourier	1358:1364	arg1	transform					1366:1374	transform	1366:1374	transform infrared (FTIR) study	1366:1396	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	3	40	dep	methylcellulose	503:517	arg1	L-HPC					591:595	L-HPC	591:595	L-HPC	591:595	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	40	dep	methylcellulose	503:517	arg1	PVA					545:547	PVA	545:547	PVA	545:547	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	40	dep	methylcellulose	503:517	arg1	pullulan					598:605	pullulan	598:605	pullulan	598:605	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	40	dep	methylcellulose	503:517	arg1	PU					608:609	PU	608:609	PU	608:609	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	40	dep	methylcellulose	503:517	arg1	alcohol					536:542	polyvinyl alcohol	526:542	polyvinyl alcohol	526:542	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	40	dep	methylcellulose	503:517	arg1	cellulose					580:588	low-substituted hydroxypropyl cellulose	550:588	low-substituted hydroxypropyl cellulose	550:588	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	4	41	theme	racecadotril	732:743	arg1	racecadotril					732:743	racecadotril	732:743	racecadotril	732:743	Afterwards, the study explored the maximum amount of racecadotril incorporated into PVA films and PU films.					
33389269	4	41	theme	racecadotril	732:743	arg1	amount					722:727	the maximum amount	710:727	the maximum amount of racecadotril incorporated into PVA films and PU films	710:784	Afterwards, the study explored the maximum amount of racecadotril incorporated into PVA films and PU films.					
33389269	2	42	theme	successful	382:391	arg1	properties					424:433	successful mechanical and physicochemical properties	382:433	successful mechanical and physicochemical properties	382:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	0	43	theme	In	13:14	arg1	Development					0:10	Development	0:10	Development	0:10	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	0	43	theme	In	13:14	arg1	Evaluation					34:43	In Vitro and In Vivo Evaluation	13:43	In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use	13:98	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	1	44	theme	pediatric	263:271	arg1	patients					273:280	pediatric patients	263:280	pediatric patients	263:280	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	7	45	theme	vitro	1414:1418	arg1	study					1432:1436	comparative in vitro dissolution study	1399:1436	comparative in vitro dissolution study	1399:1436	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	46	theme	comparative	1399:1409	arg1	study					1432:1436	comparative in vitro dissolution study	1399:1436	comparative in vitro dissolution study	1399:1436	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	3	47	theme	film-forming	656:667	arg1	polymers					669:676	stable and safe film-forming polymers	640:676	stable and safe film-forming polymers	640:676	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	4	48	theme	PU	777:778	arg1	films					780:784	PU films	777:784	PU films	777:784	Afterwards, the study explored the maximum amount of racecadotril incorporated into PVA films and PU films.					
33389269	9	49	dep	showed	1827:1832	arg1	bioequivalent					1879:1891	bioequivalent	1879:1891	bioequivalent	1879:1891	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	1	50	theme	pediatric	203:211	arg1	use					213:215	pediatric use	203:215	pediatric use	203:215	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	5	51	theme	PVA	931:933	arg1	films					935:939	PVA films	931:939	PVA films	931:939	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	5	52	dep	Lycoat	815:820	arg1	w/w					836:838	w/w	836:838	w/w	836:838	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	5	52	dep	Lycoat	815:820	arg1	%					833:833	4-10%	829:833	4-10%	829:833	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	7	53	theme	infrared	1376:1383	arg1	study					1392:1396	infrared (FTIR) study	1376:1396	infrared (FTIR) study	1376:1396	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	6	54	theme	tensile	1037:1043	arg1	strength					1045:1052	tensile strength	1037:1052	tensile strength	1037:1052	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	54	theme	tensile	1037:1043	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	55	theme	prepared	1188:1195	arg1	ODFs					1197:1200	prepared ODFs	1188:1200	prepared ODFs	1188:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	3	56	theme	hydroxypropyl	489:501	arg1	methylcellulose					503:517	hydroxypropyl methylcellulose	489:517	hydroxypropyl methylcellulose	489:517	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	56	theme	hydroxypropyl	489:501	arg1	HPMC					520:523	HPMC	520:523	HPMC	520:523	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	10	57	contain	containing	1926:1935	arg1	ODFs					1921:1924	ODFs	1921:1924	ODFs containing 30 mg racecadotril	1921:1954	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	10	57	contain	containing	1926:1935	arg2	racecadotril					1943:1954	30 mg racecadotril	1937:1954	30 mg racecadotril	1937:1954	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	2	58	with	ODFs	372:375	arg1	properties					424:433	successful mechanical and physicochemical properties	382:433	successful mechanical and physicochemical properties	382:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	10	59	theme	pediatric	2060:2068	arg1	patients					2070:2077	the pediatric patients	2056:2077	the pediatric patients	2056:2077	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	8	60	dep	achieve	1660:1666	arg1	releasing					1741:1749	releasing	1741:1749	releasing more than 85% drug within 5 min in four dissolution media	1741:1807	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	8	60	dep	achieve	1660:1666	arg1	disintegrating					1701:1714	disintegrating	1701:1714	disintegrating within a few seconds	1701:1735	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	7	61	theme	X-ray	1327:1331	arg1	XRD					1346:1348	XRD	1346:1348	XRD	1346:1348	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	61	theme	X-ray	1327:1331	arg1	diffraction					1333:1343	powder X-ray diffraction	1320:1343	powder X-ray diffraction (XRD) study	1320:1355	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	9	62	from	dogs	1903:1906	arg1	bioequivalent					1879:1891	bioequivalent	1879:1891	bioequivalent	1879:1891	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	8	63	theme	few	1725:1727	arg1	seconds					1729:1735	a few seconds	1723:1735	a few seconds	1723:1735	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	3	64	theme	low-substituted	550:564	arg1	L-HPC					591:595	L-HPC	591:595	L-HPC	591:595	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	64	theme	low-substituted	550:564	arg1	cellulose					580:588	low-substituted hydroxypropyl cellulose	550:588	low-substituted hydroxypropyl cellulose	550:588	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	9	65	theme	Beagle	1896:1901	arg1	dogs					1903:1906	Beagle dogs	1896:1906	Beagle dogs	1896:1906	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	1	66	theme	30	180:181	arg1	mg					183:184	mg	183:184	mg	183:184	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	6	67	theme	disintegration	1075:1088	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	67	theme	disintegration	1075:1088	arg1	time					1090:1093	disintegration time	1075:1093	disintegration time	1075:1093	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	10	68	theme	mg	1940:1941	arg1	racecadotril					1943:1954	30 mg racecadotril	1937:1954	30 mg racecadotril	1937:1954	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	7	69	theme	racecadotril	1493:1504	arg1	ODFs					1506:1509	optimized racecadotril ODFs	1483:1509	optimized racecadotril ODFs	1483:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	10	70	theme	solvent	1986:1992	arg1	method					2002:2007	solvent casting method	1986:2007	solvent casting method	1986:2007	In summary, ODFs containing 30 mg racecadotril were successfully prepared by solvent casting method, and it was suitable for the administration to the pediatric patients.					
33389269	9	71	from	bioequivalent	1879:1891	arg1	dogs					1903:1906	Beagle dogs	1896:1906	Beagle dogs	1896:1906	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	7	72	theme	scanning	1286:1293	arg1	DSC					1308:1310	DSC	1308:1310	DSC	1308:1310	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	72	theme	scanning	1286:1293	arg1	calorimetry					1295:1305	Differential scanning calorimetry	1273:1305	Differential scanning calorimetry (DSC) study	1273:1317	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	8	73	dep	%	1562:1562	arg1	racecadotril					1564:1575	racecadotril	1564:1575	50% racecadotril	1560:1575	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	5	74	theme	films	975:979	arg1	flexibility					957:967	the flexibility	953:967	the flexibility of PU films, respectively	953:993	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	7	75	theme	Beagle	1468:1473	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	6	76	theme	pH	1129:1130	arg1	value					1132:1136	pH value	1129:1136	pH value	1129:1136	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	76	theme	pH	1129:1130	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	0	77	theme	Racecadotril	48:59	arg1	Films					76:80	Racecadotril Orodispersible Films	48:80	Racecadotril Orodispersible Films	48:80	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	8	78	theme	dissolution	1791:1801	arg1	media					1803:1807	four dissolution media	1786:1807	four dissolution media	1786:1807	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	0	79	theme	Films	76:80	arg1	Development					0:10	Development	0:10	Development	0:10	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	0	79	theme	Films	76:80	arg1	Evaluation					34:43	In Vitro and In Vivo Evaluation	13:43	In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use	13:98	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	1	80	theme	orodispersible	141:154	arg1	ODFs					163:166	ODFs	163:166	ODFs	163:166	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	1	80	theme	orodispersible	141:154	arg1	films					156:160	orodispersible films	141:160	orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking	141:309	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	9	81	theme	in	1813:1814	arg1	study					1821:1825	An in vivo study	1810:1825	An in vivo study	1810:1825	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	0	82	theme	Pediatric	86:94	arg1	Use					96:98	Pediatric Use	86:98	Pediatric Use	86:98	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	0	83	dep	In	13:14	arg1	Vitro					16:20	Vitro	16:20	Vitro	16:20	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	8	84	dep	%	1588:1588	arg1	Lycoat					1590:1595	Lycoat RS720	1590:1601	7% Lycoat RS720	1587:1601	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	6	85	theme	racecadotril	1254:1265	arg1	ODFs					1267:1270	racecadotril ODFs	1254:1270	racecadotril ODFs	1254:1270	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	8	86	theme	1	1634:1634	arg1	%					1635:1635	%	1635:1635	%	1635:1635	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	3	87	dep	polymers	479:486	arg1	methylcellulose					503:517	hydroxypropyl methylcellulose	489:517	hydroxypropyl methylcellulose	489:517	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	87	dep	polymers	479:486	arg1	HPMC					520:523	HPMC	520:523	HPMC	520:523	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	9	88	theme	optimized	1834:1842	arg1	ODF					1857:1859	optimized racecadotril ODF	1834:1859	optimized racecadotril ODF	1834:1859	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	4	89	theme	PVA	763:765	arg1	films					767:771	PVA films	763:771	PVA films	763:771	Afterwards, the study explored the maximum amount of racecadotril incorporated into PVA films and PU films.					
33389269	2	90	theme	high	354:357	arg1	ODFs					372:375	high drug loading ODFs	354:375	high drug loading ODFs with successful mechanical and physicochemical properties	354:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	8	91	theme	titanium	1637:1644	arg1	dioxide					1646:1652	1% titanium dioxide	1634:1652	1% titanium dioxide	1634:1652	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	6	92	theme	Formulation	996:1006	arg1	value					1132:1136	pH value	1129:1136	pH value	1129:1136	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	elongation					1063:1072	percent elongation	1055:1072	percent elongation	1055:1072	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	uptake					1166:1171	moisture uptake	1157:1171	moisture uptake	1157:1171	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	content					1101:1107	drug content	1096:1107	drug content	1096:1107	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	appearance					1025:1034	appearance	1025:1034	appearance	1025:1034	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	weight					1110:1115	weight	1110:1115	weight	1110:1115	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	strength					1045:1052	tensile strength	1037:1052	tensile strength	1037:1052	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	content					1148:1154	moisture content	1139:1154	moisture content	1139:1154	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	time					1090:1093	disintegration time	1075:1093	disintegration time	1075:1093	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	Q5min					1178:1182	Q5min	1178:1182	Q5min	1178:1182	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	thickness					1118:1126	thickness	1118:1126	thickness	1118:1126	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	92	theme	Formulation	996:1006	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	2	93	theme	physicochemical	408:422	arg1	properties					424:433	successful mechanical and physicochemical properties	382:433	successful mechanical and physicochemical properties	382:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	8	94	dep	%	1580:1580	arg1	PVA					1582:1584	PVA	1582:1584	38% PVA	1578:1584	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	4	95	theme	maximum	714:720	arg1	racecadotril					732:743	racecadotril	732:743	racecadotril	732:743	Afterwards, the study explored the maximum amount of racecadotril incorporated into PVA films and PU films.					
33389269	4	95	theme	maximum	714:720	arg1	amount					722:727	the maximum amount	710:727	the maximum amount of racecadotril incorporated into PVA films and PU films	710:784	Afterwards, the study explored the maximum amount of racecadotril incorporated into PVA films and PU films.					
33389269	7	96	from	study	1432:1436	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	2	97	theme	mechanical	393:402	arg1	properties					424:433	successful mechanical and physicochemical properties	382:433	successful mechanical and physicochemical properties	382:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	5	98	theme	disintegration	908:921	arg1	time					923:926	disintegration time	908:926	disintegration time of PVA films	908:939	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	1	99	contain	containing	169:178	arg2	racecadotril					186:197	30 mg racecadotril	180:197	30 mg racecadotril	180:197	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	1	99	contain	containing	169:178	arg1	ODFs					163:166	ODFs	163:166	ODFs	163:166	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	1	99	contain	containing	169:178	arg1	films					156:160	orodispersible films	141:160	orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking	141:309	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	6	100	dep	characteristics	1008:1022	arg1	value					1132:1136	pH value	1129:1136	pH value	1129:1136	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	elongation					1063:1072	percent elongation	1055:1072	percent elongation	1055:1072	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	uptake					1166:1171	moisture uptake	1157:1171	moisture uptake	1157:1171	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	content					1101:1107	drug content	1096:1107	drug content	1096:1107	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	appearance					1025:1034	appearance	1025:1034	appearance	1025:1034	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	weight					1110:1115	weight	1110:1115	weight	1110:1115	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	strength					1045:1052	tensile strength	1037:1052	tensile strength	1037:1052	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	content					1148:1154	moisture content	1139:1154	moisture content	1139:1154	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	time					1090:1093	disintegration time	1075:1093	disintegration time	1075:1093	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	Q5min					1178:1182	Q5min	1178:1182	Q5min	1178:1182	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	thickness					1118:1126	thickness	1118:1126	thickness	1118:1126	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	100	dep	characteristics	1008:1022	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	0	101	theme	In	26:27	arg1	Development					0:10	Development	0:10	Development	0:10	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	0	101	theme	In	26:27	arg1	Evaluation					34:43	In Vitro and In Vivo Evaluation	13:43	In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use	13:98	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	1	102	theme	patients	273:280	arg1	compliance					249:258	the compliance	245:258	the compliance of pediatric patients	245:280	The present study endeavored to develop orodispersible films (ODFs) containing 30 mg racecadotril for pediatric use, which focuses on improving the compliance of pediatric patients and reducing risk of choking.					
33389269	7	103	theme	dissolution	1420:1430	arg1	study					1432:1436	comparative in vitro dissolution study	1399:1436	comparative in vitro dissolution study	1399:1436	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	2	104	theme	loading	364:370	arg1	ODFs					372:375	high drug loading ODFs	354:375	high drug loading ODFs with successful mechanical and physicochemical properties	354:433	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33389269	7	105	from	study	1459:1463	arg1	dogs					1475:1478	Beagle dogs	1468:1478	Beagle dogs of optimized racecadotril ODFs	1468:1509	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	7	106	theme	in	1411:1412	arg1	study					1432:1436	comparative in vitro dissolution study	1399:1436	comparative in vitro dissolution study	1399:1436	Differential scanning calorimetry (DSC) study, powder X-ray diffraction (XRD) study, Fourier transform infrared (FTIR) study, comparative in vitro dissolution study, and pharmacokinetic study in Beagle dogs of optimized racecadotril ODFs were then conducted.					
33389269	0	107	dep	In	26:27	arg1	Vivo					29:32	Vivo	29:32	Vivo	29:32	Development, In Vitro and In Vivo Evaluation of Racecadotril Orodispersible Films for Pediatric Use.					
33389269	3	108	theme	safe	651:654	arg1	polymers					669:676	stable and safe film-forming polymers	640:676	stable and safe film-forming polymers	640:676	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	6	109	theme	percent	1055:1061	arg1	characteristics					1008:1022	Formulation characteristics	996:1022	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs	996:1200	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	6	109	theme	percent	1055:1061	arg1	elongation					1063:1072	percent elongation	1055:1072	percent elongation	1055:1072	Formulation characteristics (appearance, tensile strength, percent elongation, disintegration time, drug content, weight, thickness, pH value, moisture content, moisture uptake, and Q5min) of prepared ODFs were examined to obtain the optimal compositions of racecadotril ODFs.					
33389269	8	110	dep	%	1619:1619	arg1	apricot					1621:1627	apricot	1621:1627	2% apricot	1618:1627	Eventually, ODFs containing 50% racecadotril, 38% PVA, 7% Lycoat RS720, 2% sucralose, 2% apricot, and 1% titanium dioxide could achieve desirable mechanical properties, disintegrating within a few seconds and releasing more than 85% drug within 5 min in four dissolution media.					
33389269	3	111	theme	stable	640:645	arg1	polymers					669:676	stable and safe film-forming polymers	640:676	stable and safe film-forming polymers	640:676	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	3	112	theme	different	469:477	arg1	polymers					479:486	different polymers	469:486	different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU)	469:610	Compatibilities between drug and different polymers (hydroxypropyl methylcellulose, HPMC; polyvinyl alcohol, PVA; low-substituted hydroxypropyl cellulose, L-HPC; pullulan, PU) were investigated to select stable and safe film-forming polymers.					
33389269	9	113	dep	in	1813:1814	arg1	vivo					1816:1819	vivo	1816:1819	vivo	1816:1819	An in vivo study showed optimized racecadotril ODF and Hidrasec were bioequivalent in Beagle dogs.					
33389269	5	114	theme	films	935:939	arg1	time					923:926	disintegration time	908:926	disintegration time of PVA films	908:939	Subsequently, disintegrant (Lycoat RS720, 4-10%, w/w) and plasticizers (glycerol, 2-6%, w/w) were investigated to reduce disintegration time of PVA films and enhance the flexibility of PU films, respectively.					
33389269	2	115	theme	study	334:338	arg1	challenge					316:324	The challenge	312:324	The challenge of this study	312:338	The challenge of this study is to prepare high drug loading ODFs with successful mechanical and physicochemical properties.					
33357897	5	0	theme	optical	790:796	arg1	%					816:816	93 %	813:816	93 %	813:816	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	0	theme	optical	790:796	arg1	transmittance					798:810	high optical transmittance	785:810	high optical transmittance (93 %)	785:817	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	4	1	theme	polyvinyl	728:736	arg1	PVB					747:749	PVB	747:749	PVB	747:749	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	4	1	theme	polyvinyl	728:736	arg1	butyral					738:744	polyvinyl butyral	728:744	polyvinyl butyral (PVB)	728:750	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	7	2	theme	specific	1068:1075	arg1	energy					1077:1082	13 % more specific energy	1058:1082	13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB	1058:1151	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	7	2	theme	specific	1068:1075	arg1	break					1051:1055	break	1051:1055	break	1051:1055	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	0	3	theme	petroleum-based	92:106	arg1	polymers					108:115	petroleum-based polymers	92:115	petroleum-based polymers	92:115	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	3	4	dep	modification	419:430	arg1	increase					495:502	increase	495:502	to increase the performance of the films as well as to improve the compatibility within conventional polymer	492:599	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	3	4	dep	modification	419:430	arg1	improve					547:553	improve	547:553	to increase the performance of the films as well as to improve the compatibility within conventional polymer	492:599	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	3	5	theme	conventional	580:591	arg1	polymer					593:599	conventional polymer	580:599	conventional polymer	580:599	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	7	6	contain	has	1016:1018	arg1	composite					1006:1014	PVB composite	1002:1014	PVB composite	1002:1014	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	7	6	contain	has	1016:1018	arg2	elongation					1037:1046	three times more elongation	1020:1046	three times more elongation	1020:1046	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	5	7	theme	NFC	766:768	arg1	83°					871:873	83°	871:873	83°	871:873	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	7	theme	NFC	766:768	arg1	hydrophobic					858:868	hydrophobic	858:868	hydrophobic	858:868	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	7	theme	NFC	766:768	arg1	films					770:774	The modified NFC films	753:774	The modified NFC films	753:774	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	3	8	theme	films	527:531	arg1	performance					508:518	the performance	504:518	the performance of the films	504:531	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	2	9	theme	hydrophilic	294:304	arg1	brittle					280:286	brittle	280:286	brittle	280:286	However, they are brittle, very hydrophilic, which is decreasing their potential applications.					
33357897	2	9	theme	hydrophilic	294:304	arg1	they					271:274	they	271:274	they	271:274	However, they are brittle, very hydrophilic, which is decreasing their potential applications.					
33357897	7	10	dep	NFC	996:998	arg1	has					1016:1018	has	1016:1018	has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB	1016:1151	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	1	11	theme	interesting	187:197	arg1	strength					252:259	high mechanical strength	236:259	high mechanical strength	236:259	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	1	11	theme	interesting	187:197	arg1	proprieties					199:209	their interesting proprieties	181:209	their interesting proprieties such as transparency and high mechanical strength	181:259	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	1	11	theme	interesting	187:197	arg1	transparency					219:230	transparency	219:230	transparency	219:230	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	7	12	theme	half-tensile	1100:1111	arg1	stress					1113:1118	a half-tensile stress	1098:1118	a half-tensile stress compared to an interlayer of PVB	1098:1151	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	6	13	theme	higher	879:884	arg1	flexibility					886:896	A higher flexibility	877:896	A higher flexibility	877:896	A higher flexibility was also achieved, as the film was greatly stretched and bended without cracking or breaking.					
33357897	7	14	theme	more	1063:1066	arg1	energy					1077:1082	13 % more specific energy	1058:1082	13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB	1058:1151	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	7	14	theme	more	1063:1066	arg1	break					1051:1055	break	1051:1055	break	1051:1055	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	4	15	theme	safety	683:688	arg1	glazing					690:696	safety glazing	683:696	safety glazing	683:696	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	3	16	theme	chemical	410:417	arg1	modification					419:430	a simple and effective chemical modification	387:430	a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer	387:599	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	2	17	theme	potential	333:341	arg1	applications					343:354	their potential applications	327:354	their potential applications	327:354	However, they are brittle, very hydrophilic, which is decreasing their potential applications.					
33357897	4	18	theme	bulletproof	705:715	arg1	glass					717:721	bulletproof glass	705:721	bulletproof glass	705:721	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	5	19	theme	tensile	830:836	arg1	stretch					838:844	tensile stretch	830:844	tensile stretch	830:844	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	4	20	from	interlayer	669:678	arg1	glazing					690:696	safety glazing	683:696	safety glazing	683:696	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	4	20	from	interlayer	669:678	arg1	glass					717:721	bulletproof glass	705:721	bulletproof glass	705:721	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	3	21	theme	effective	400:408	arg1	modification					419:430	a simple and effective chemical modification	387:430	a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer	387:599	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	4	22	theme	preliminary	604:614	arg1	study					616:620	A preliminary study	602:620	A preliminary study	602:620	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	1	23	theme	Nanofibrillated	118:132	arg1	films					144:148	Nanofibrillated cellulose films	118:148	Nanofibrillated cellulose films	118:148	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	3	24	theme	polymer	441:447	arg1	grafting					449:456	polymer grafting	441:456	polymer grafting	441:456	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	7	25	theme	more	1032:1035	arg1	elongation					1037:1046	three times more elongation	1020:1046	three times more elongation	1020:1046	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	1	26	theme	high	236:239	arg1	strength					252:259	high mechanical strength	236:259	high mechanical strength	236:259	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	0	27	theme	transparent	24:34	arg1	films					75:79	transparent and flexible nanofibrillated cellulose films	24:79	transparent and flexible nanofibrillated cellulose films	24:79	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	5	28	theme	modified	757:764	arg1	83°					871:873	83°	871:873	83°	871:873	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	28	theme	modified	757:764	arg1	hydrophobic					858:868	hydrophobic	858:868	hydrophobic	858:868	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	28	theme	modified	757:764	arg1	films					770:774	The modified NFC films	753:774	The modified NFC films	753:774	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	1	29	theme	mechanical	241:250	arg1	strength					252:259	high mechanical strength	236:259	high mechanical strength	236:259	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	0	30	theme	nanofibrillated	49:63	arg1	films					75:79	transparent and flexible nanofibrillated cellulose films	24:79	transparent and flexible nanofibrillated cellulose films	24:79	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	3	31	theme	plasticizer	470:480	arg1	additions					482:490	plasticizer additions	470:490	plasticizer additions	470:490	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33357897	7	32	theme	PVB	1002:1004	arg1	composite					1006:1014	PVB composite	1002:1014	PVB composite	1002:1014	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	1	33	theme	cellulose	134:142	arg1	films					144:148	Nanofibrillated cellulose films	118:148	Nanofibrillated cellulose films	118:148	Nanofibrillated cellulose films have garnered attention due to their interesting proprieties such as transparency and high mechanical strength.					
33357897	0	34	theme	flexible	40:47	arg1	films					75:79	transparent and flexible nanofibrillated cellulose films	24:79	transparent and flexible nanofibrillated cellulose films	24:79	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	4	35	with	interlayer	669:678	arg1	PVB					747:749	PVB	747:749	PVB	747:749	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	4	35	with	interlayer	669:678	arg1	butyral					738:744	polyvinyl butyral	728:744	polyvinyl butyral (PVB)	728:750	A preliminary study shows the possibility of using this film as an interlayer in safety glazing and/or bulletproof glass with polyvinyl butyral (PVB).					
33357897	5	36	theme	high	785:788	arg1	%					816:816	93 %	813:816	93 %	813:816	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	36	theme	high	785:788	arg1	transmittance					798:810	high optical transmittance	785:810	high optical transmittance (93 %)	785:817	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	37	dep	hydrophobic	858:868	arg1	83°					871:873	83°	871:873	83°	871:873	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	37	dep	hydrophobic	858:868	arg1	hydrophobic					858:868	hydrophobic	858:868	hydrophobic	858:868	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	5	37	dep	hydrophobic	858:868	arg1	films					770:774	The modified NFC films	753:774	The modified NFC films	753:774	The modified NFC films displays high optical transmittance (93 %), increases tensile stretch and is more hydrophobic (83°).					
33357897	0	38	theme	films	75:79	arg1	synthesis					11:19	synthesis	11:19	synthesis	11:19	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	0	38	theme	films	75:79	arg1	Design					0:5	Design	0:5	Design	0:5	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	0	39	theme	cellulose	65:73	arg1	films					75:79	transparent and flexible nanofibrillated cellulose films	24:79	transparent and flexible nanofibrillated cellulose films	24:79	Design and synthesis of transparent and flexible nanofibrillated cellulose films to replace petroleum-based polymers.					
33357897	7	40	theme	PVB	1149:1151	arg1	interlayer					1135:1144	an interlayer	1132:1144	an interlayer of PVB	1132:1151	The NFC / PVB composite has three times more elongation at break, 13 % more specific energy absorbed with a half-tensile stress compared to an interlayer of PVB.					
33357897	3	41	theme	simple	389:394	arg1	modification					419:430	a simple and effective chemical modification	387:430	a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer	387:599	We have successfully achieved a simple and effective chemical modification based on polymer grafting and through plasticizer additions to increase the performance of the films as well as to improve the compatibility within conventional polymer.					
33712123	0	0	theme	bone	112:115	arg1	engineering					124:134	bone tissue engineering	112:134	bone tissue engineering	112:134	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	2	1	theme	true	506:509	arg1	microscopy					526:535	true color confocal microscopy	506:535	true color confocal microscopy	506:535	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	4	2	theme	sizes	924:928	arg1	μm					934:935	microscopic pore sizes ≤10 μm	907:935	microscopic pore sizes ≤10 μm	907:935	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	2	3	theme	CS/HA/braid	345:355	arg1	scaffold					357:364	The CS/HA/braid scaffold	341:364	The CS/HA/braid scaffold with hierarchical pore structure	341:397	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	1	4	theme	structure	246:254	arg1	scaffold					256:263	a hierarchical pore structure scaffold	226:263	a hierarchical pore structure scaffold	226:263	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	3	5	theme	degradation	725:735	arg1	test					737:740	degradation test	725:740	degradation test	725:740	The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test.					
33712123	2	6	dep	Fourier	465:471	arg1	transform					473:481	transform	473:481	transform infrared spectroscopy	473:503	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	8	7	theme	synergistic	1228:1238	arg1	effect					1240:1245	the synergistic effect	1224:1245	the synergistic effect of HA and CAP treatment	1224:1269	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	6	8	theme	mechanical	1087:1096	arg1	properties					1098:1107	good mechanical properties	1082:1107	good mechanical properties	1082:1107	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	4	9	with	endowed	868:874	arg1	μm					934:935	microscopic pore sizes ≤10 μm	907:935	microscopic pore sizes ≤10 μm	907:935	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	9	10	theme	hierarchical	1414:1425	arg1	structure					1432:1440	hierarchical pore structure	1414:1440	hierarchical pore structure	1414:1440	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	2	11	theme	liquid	547:552	arg1	method					566:571	improved liquid replacement method	538:571	improved liquid replacement method	538:571	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	4	12	theme	CAP	854:856	arg1	treatment					858:866	CAP treatment	854:866	CAP treatment	854:866	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	4	13	theme	scaffold	799:806	arg1	core					787:790	the core	783:790	the core of the scaffold	783:806	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	9	14	theme	pore	1427:1430	arg1	structure					1432:1440	hierarchical pore structure	1414:1440	hierarchical pore structure	1414:1440	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	0	15	theme	tissue	117:122	arg1	engineering					124:134	bone tissue engineering	112:134	bone tissue engineering	112:134	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	4	16	theme	scaffold	893:900	arg1	shell					880:884	the shell	876:884	the shell of the scaffold	876:900	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	8	17	theme	CS	1333:1334	arg1	scaffold					1336:1343	pure CS scaffold	1328:1343	pure CS scaffold	1328:1343	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	8	18	theme	cell	1299:1302	arg1	viability					1304:1312	277.6 % cell viability	1291:1312	277.6 % cell viability	1291:1312	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	7	19	theme	spherical	1195:1203	arg1	crystals					1208:1215	intact spherical HA crystals	1188:1215	intact spherical HA crystals	1188:1215	In addition, the treated scaffold precipitated intact spherical HA crystals.					
33712123	9	20	theme	bone	1394:1397	arg1	scaffolds					1399:1407	bone scaffolds	1394:1407	bone scaffolds with hierarchical pore structure for potential bone tissue engineering	1394:1478	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	8	21	theme	treatment	1261:1269	arg1	effect					1240:1245	the synergistic effect	1224:1245	the synergistic effect of HA and CAP treatment	1224:1269	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	4	22	theme	macroscopic	813:823	arg1	sizes					830:834	macroscopic pore sizes	813:834	macroscopic pore sizes of 80-180 μm	813:847	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	8	23	theme	CAP	1257:1259	arg1	treatment					1261:1269	CAP treatment	1257:1269	CAP treatment	1257:1269	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	6	24	theme	good	1082:1085	arg1	properties					1098:1107	good mechanical properties	1082:1107	good mechanical properties	1082:1107	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	7	25	theme	HA	1205:1206	arg1	crystals					1208:1215	intact spherical HA crystals	1188:1215	intact spherical HA crystals	1188:1215	In addition, the treated scaffold precipitated intact spherical HA crystals.					
33712123	7	26	theme	intact	1188:1193	arg1	crystals					1208:1215	intact spherical HA crystals	1188:1215	intact spherical HA crystals	1188:1215	In addition, the treated scaffold precipitated intact spherical HA crystals.					
33712123	9	27	theme	potential	1446:1454	arg1	engineering					1468:1478	potential bone tissue engineering	1446:1478	potential bone tissue engineering	1446:1478	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	2	28	theme	infrared	483:490	arg1	spectroscopy					492:503	infrared spectroscopy	483:503	infrared spectroscopy	483:503	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	4	29	theme	microscopic	907:917	arg1	μm					934:935	microscopic pore sizes ≤10 μm	907:935	microscopic pore sizes ≤10 μm	907:935	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	2	30	theme	pore	384:387	arg1	structure					389:397	hierarchical pore structure	371:397	hierarchical pore structure	371:397	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	0	31	theme	Two-step	0:7	arg1	strategy					9:16	Two-step strategy	0:16	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite	0:96	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	3	32	theme	compression	704:714	arg1	test					716:719	compression test	704:719	compression test	704:719	The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test.					
33712123	5	33	theme	high	962:965	arg1	rates					989:993	high porosity and swelling rates	962:993	high porosity and swelling rates of ≥80 % and ≥300 %, respectively	962:1027	All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively.					
33712123	2	34	theme	hierarchical	371:382	arg1	structure					389:397	hierarchical pore structure	371:397	hierarchical pore structure	371:397	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	2	35	theme	electronic	442:451	arg1	microscopy					453:462	scanning electronic microscopy	433:462	scanning electronic microscopy	433:462	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	1	36	theme	freeze	281:286	arg1	drying					288:293	freeze drying	281:293	freeze drying	281:293	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	9	37	with	scaffolds	1399:1407	arg1	structure					1432:1440	hierarchical pore structure	1414:1440	hierarchical pore structure	1414:1440	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	5	38	theme	porosity	967:974	arg1	rates					989:993	high porosity and swelling rates	962:993	high porosity and swelling rates of ≥80 % and ≥300 %, respectively	962:1027	All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively.					
33712123	9	39	theme	bone	1456:1459	arg1	engineering					1468:1478	potential bone tissue engineering	1446:1478	potential bone tissue engineering	1446:1478	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	5	40	theme	swelling	980:987	arg1	rates					989:993	high porosity and swelling rates	962:993	high porosity and swelling rates of ≥80 % and ≥300 %, respectively	962:1027	All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively.					
33712123	2	41	theme	scanning	433:440	arg1	microscopy					453:462	scanning electronic microscopy	433:462	scanning electronic microscopy	433:462	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	8	42	theme	pure	1328:1331	arg1	scaffold					1336:1343	pure CS scaffold	1328:1343	pure CS scaffold	1328:1343	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	1	43	theme	composite	205:213	arg1	braid					215:219	a composite braid	203:219	a composite braid	203:219	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	0	44	theme	pore	48:51	arg1	composite					88:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	5	45	theme	%	1013:1013	arg1	rates					989:993	high porosity and swelling rates	962:993	high porosity and swelling rates of ≥80 % and ≥300 %, respectively	962:1027	All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively.					
33712123	3	46	theme	mechanical	619:628	arg1	properties					630:639	The mechanical properties	615:639	The mechanical properties	615:639	The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test.					
33712123	6	47	theme	pore	1063:1066	arg1	structure					1068:1076	a hierarchical pore structure	1048:1076	a hierarchical pore structure	1048:1076	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	0	48	theme	hierarchical	35:46	arg1	composite					88:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	1	49	theme	cold	299:302	arg1	technology					329:338	cold atmospheric plasma (CAP) technology	299:338	cold atmospheric plasma (CAP) technology	299:338	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	2	50	theme	replacement	554:564	arg1	method					566:571	improved liquid replacement method	538:571	improved liquid replacement method	538:571	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	4	51	theme	μm	846:847	arg1	sizes					830:834	macroscopic pore sizes	813:834	macroscopic pore sizes of 80-180 μm	813:847	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	7	52	theme	treated	1158:1164	arg1	scaffold					1166:1173	the treated scaffold	1154:1173	the treated scaffold	1154:1173	In addition, the treated scaffold precipitated intact spherical HA crystals.					
33712123	0	53	theme	chitosan-hydroxyapatite	64:86	arg1	composite					88:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	1	54	theme	atmospheric	304:314	arg1	technology					329:338	cold atmospheric plasma (CAP) technology	299:338	cold atmospheric plasma (CAP) technology	299:338	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	4	55	theme	pore	919:922	arg1	μm					934:935	microscopic pore sizes ≤10 μm	907:935	microscopic pore sizes ≤10 μm	907:935	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	3	56	theme	scaffold	672:679	arg1	properties					630:639	The mechanical properties	615:639	The mechanical properties	615:639	The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test.					
33712123	3	56	theme	scaffold	672:679	arg1	ability					657:663	degradation ability	645:663	degradation ability	645:663	The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test.					
33712123	2	57	theme	improved	538:545	arg1	method					566:571	improved liquid replacement method	538:571	improved liquid replacement method	538:571	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	8	58	theme	%	1297:1297	arg1	viability					1304:1312	277.6 % cell viability	1291:1312	277.6 % cell viability	1291:1312	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	0	59	theme	structured	53:62	arg1	composite					88:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	hierarchical pore structured chitosan-hydroxyapatite composite	35:96	Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.					
33712123	1	60	theme	plasma	316:321	arg1	technology					329:338	cold atmospheric plasma (CAP) technology	299:338	cold atmospheric plasma (CAP) technology	299:338	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	2	61	theme	solution	595:602	arg1	immersion					604:612	phosphate buffer solution immersion	578:612	phosphate buffer solution immersion	578:612	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	8	62	theme	277.6	1291:1295	arg1	%					1297:1297	%	1297:1297	%	1297:1297	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	4	63	with	endowed	775:781	arg1	sizes					830:834	macroscopic pore sizes	813:834	macroscopic pore sizes of 80-180 μm	813:847	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	2	64	theme	buffer	588:593	arg1	immersion					604:612	phosphate buffer solution immersion	578:612	phosphate buffer solution immersion	578:612	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	6	65	with	scaffold	1034:1041	arg1	structure					1068:1076	a hierarchical pore structure	1048:1076	a hierarchical pore structure	1048:1076	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	5	66	theme	%	1002:1002	arg1	rates					989:993	high porosity and swelling rates	962:993	high porosity and swelling rates of ≥80 % and ≥300 %, respectively	962:1027	All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively.					
33712123	6	67	theme	degradation	1123:1133	arg1	rate					1135:1138	twice the degradation rate	1113:1138	twice the degradation rate	1113:1138	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	9	68	theme	tissue	1461:1466	arg1	engineering					1468:1478	potential bone tissue engineering	1446:1478	potential bone tissue engineering	1446:1478	Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.					
33712123	2	69	theme	phosphate	578:586	arg1	immersion					604:612	phosphate buffer solution immersion	578:612	phosphate buffer solution immersion	578:612	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	4	70	theme	pore	825:828	arg1	sizes					830:834	macroscopic pore sizes	813:834	macroscopic pore sizes of 80-180 μm	813:847	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	4	71	theme	HA	763:764	arg1	addition					766:773	HA addition	763:773	HA addition	763:773	Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 μm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 μm.					
33712123	2	72	theme	confocal	517:524	arg1	microscopy					526:535	true color confocal microscopy	506:535	true color confocal microscopy	506:535	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	2	73	with	scaffold	357:364	arg1	structure					389:397	hierarchical pore structure	371:397	hierarchical pore structure	371:397	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	6	74	theme	hierarchical	1050:1061	arg1	structure					1068:1076	a hierarchical pore structure	1048:1076	a hierarchical pore structure	1048:1076	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	1	75	theme	hierarchical	228:239	arg1	scaffold					256:263	a hierarchical pore structure scaffold	226:263	a hierarchical pore structure scaffold	226:263	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	2	76	theme	color	511:515	arg1	microscopy					526:535	true color confocal microscopy	506:535	true color confocal microscopy	506:535	The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion.					
33712123	1	77	theme	CAP	324:326	arg1	technology					329:338	cold atmospheric plasma (CAP) technology	299:338	cold atmospheric plasma (CAP) technology	299:338	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33712123	3	78	theme	degradation	645:655	arg1	ability					657:663	degradation ability	645:663	degradation ability	645:663	The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test.					
33712123	8	79	theme	HA	1250:1251	arg1	effect					1240:1245	the synergistic effect	1224:1245	the synergistic effect of HA and CAP treatment	1224:1269	Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold.					
33712123	6	80	contain	had	1078:1080	arg1	scaffold					1034:1041	The scaffold	1030:1041	The scaffold with a hierarchical pore structure	1030:1076	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	6	80	contain	had	1078:1080	arg2	rate					1135:1138	twice the degradation rate	1113:1138	twice the degradation rate	1113:1138	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	6	80	contain	had	1078:1080	arg2	properties					1098:1107	good mechanical properties	1082:1107	good mechanical properties	1082:1107	The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate.					
33712123	1	81	theme	pore	241:244	arg1	scaffold					256:263	a hierarchical pore structure scaffold	226:263	a hierarchical pore structure scaffold	226:263	Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology.					
33811724	0	0	theme	controlled	106:115	arg1	release					128:134	controlled antibiotic release	106:134	controlled antibiotic release	106:134	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	4	1	theme	comparable	642:651	arg1	values					618:623	values	618:623	values	618:623	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	5	2	theme	mechanical	715:724	arg1	properties					726:735	the mechanical properties	711:735	the mechanical properties with the increase of the rate of organic components	711:787	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	5	3	theme	properties	726:735	arg1	improvement					696:706	The improvement	692:706	The improvement of the mechanical properties with the increase of the rate of organic components	692:787	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	5	4	theme	area	839:842	arg1	reduction					852:860	the reduction	848:860	the reduction in the pore volume of the specimens	848:896	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	5	4	theme	area	839:842	arg1	diminution					813:822	the diminution	809:822	the diminution of the surface area	809:842	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	3	5	theme	CS	461:462	arg1	contents					472:479	CS and CIP contents	461:479	CS and CIP contents	461:479	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	4	6	from	6 MPa	635:639	arg1	values					618:623	values	618:623	values	618:623	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	7	7	theme	significant	1122:1132	arg1	inhibition					1134:1143	significant inhibition	1122:1143	significant inhibition of Staphylococcus aureus and Escherichia coli pathogens	1122:1199	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	5	8	theme	specimens	888:896	arg1	volume					874:879	the pore volume	865:879	the pore volume of the specimens	865:896	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	8	9	theme	bone	1322:1325	arg1	repair					1327:1332	bone repair	1322:1332	bone repair	1322:1332	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	1	10	theme	solid-liquid	252:263	arg1	method					272:277	the solid-liquid mixing method	248:277	the solid-liquid mixing method	248:277	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	0	11	theme	antibiotic	117:126	arg1	release					128:134	controlled antibiotic release	106:134	controlled antibiotic release	106:134	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	7	12	theme	antibacterial	1054:1066	arg1	tests					1068:1072	in vitro antibacterial tests	1045:1072	in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP	1045:1113	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	3	13	theme	compressive	488:498	arg1	strength					500:507	the compressive strength	484:507	the compressive strength of the CIP-loaded composite	484:535	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	1	14	theme	mixing	265:270	arg1	method					272:277	the solid-liquid mixing method	248:277	the solid-liquid mixing method	248:277	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	3	15	theme	synergistic	439:449	arg1	effect					451:456	A synergistic effect	437:456	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite	437:535	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	7	16	dep	in	1045:1046	arg1	vitro					1048:1052	vitro	1048:1052	vitro	1048:1052	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	6	17	theme	antibiotic	958:967	arg1	experiments					939:949	the in vitro release experiments	918:949	the in vitro release experiments of the antibiotic	918:967	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	3	18	theme	contents	472:479	arg1	effect					451:456	A synergistic effect	437:456	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite	437:535	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	6	19	theme	controlled	995:1004	arg1	release					1006:1012	a sustained and controlled release	979:1012	a sustained and controlled release of CIP	979:1019	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	6	20	theme	other	906:910	arg1	hand					912:915	the other hand	902:915	the other hand	902:915	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	0	21	theme	composite	57:65	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	0	21	theme	composite	57:65	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	2	22	from	use	338:340	arg1	amount					365:370	a small amount	357:370	a small amount (≤5 wt%)	357:379	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	2	22	from	use	338:340	arg1	%					378:378	≤5 wt%	373:378	≤5 wt%	373:378	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	2	23	theme	small	359:363	arg1	amount					365:370	a small amount	357:370	a small amount (≤5 wt%)	357:379	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	2	23	theme	small	359:363	arg1	%					378:378	≤5 wt%	373:378	≤5 wt%	373:378	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	8	24	theme	formulated	1239:1248	arg1	material					1309:1316	a promising material	1297:1316	a promising material for bone repair and local antibiotic release for the treatment of bone infections	1297:1398	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	8	24	theme	formulated	1239:1248	arg1	composite					1250:1258	the formulated composite	1235:1258	the formulated composite with three-phase components	1235:1286	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	5	25	theme	pore	869:872	arg1	volume					874:879	the pore volume	865:879	the pore volume of the specimens	865:896	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	6	26	dep	in	922:923	arg1	vitro					925:929	vitro	925:929	vitro	925:929	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	6	27	theme	sustained	981:989	arg1	release					1006:1012	a sustained and controlled release	979:1012	a sustained and controlled release of CIP	979:1019	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	7	28	theme	biocomposite	1091:1102	arg1	HA-CS5-CIP					1104:1113	the biocomposite HA-CS5-CIP	1087:1113	the biocomposite HA-CS5-CIP	1087:1113	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	8	29	with	composite	1250:1258	arg1	components					1277:1286	three-phase components	1265:1286	three-phase components	1265:1286	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	2	30	theme	solid	304:308	arg1	use					338:340	the use	334:340	the use of chitosan in a small amount (≤5 wt%)	334:379	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	2	30	theme	solid	304:308	arg1	optimization					284:295	The optimization	280:295	The optimization of the solid to the liquid ratio	280:328	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	8	31	theme	promising	1299:1307	arg1	composite					1250:1258	the formulated composite	1235:1258	the formulated composite with three-phase components	1235:1286	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	8	31	theme	promising	1299:1307	arg1	material					1309:1316	a promising material	1297:1316	a promising material for bone repair and local antibiotic release for the treatment of bone infections	1297:1398	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	5	32	with	properties	726:735	arg1	increase					746:753	the increase	742:753	the increase of the rate of organic components	742:787	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	5	33	theme	rate	762:765	arg1	increase					746:753	the increase	742:753	the increase of the rate of organic components	742:787	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	4	34	theme	cancellous	675:684	arg1	bone					686:689	cancellous bone	675:689	cancellous bone	675:689	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	1	35	from	based	149:153	arg1	CS					192:193	CS	192:193	CS	192:193	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	1	35	from	based	149:153	arg1	HA					174:175	HA	174:175	HA	174:175	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	1	35	from	based	149:153	arg1	chitosan					182:189	chitosan	182:189	chitosan (CS)	182:194	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	1	35	from	based	149:153	arg1	hydroxyapatite					158:171	hydroxyapatite	158:171	hydroxyapatite (HA)	158:176	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	8	36	theme	three-phase	1265:1275	arg1	components					1277:1286	three-phase components	1265:1286	three-phase components	1265:1286	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	5	37	from	reduction	852:860	arg1	volume					874:879	the pore volume	865:879	the pore volume of the specimens	865:896	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	4	38	theme	fabricated	584:593	arg1	biocomposite					595:606	the fabricated biocomposite	580:606	the fabricated biocomposite	580:606	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	3	39	theme	CIP-loaded	516:525	arg1	composite					527:535	the CIP-loaded composite	512:535	the CIP-loaded composite	512:535	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	4	40	dep	6 MPa	635:639	arg1	to					632:633	to	632:633	to	632:633	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	8	41	theme	infections	1389:1398	arg1	treatment					1371:1379	the treatment	1367:1379	the treatment of bone infections	1367:1398	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	7	42	theme	coli	1186:1189	arg1	inhibition					1134:1143	significant inhibition	1122:1143	significant inhibition of Staphylococcus aureus and Escherichia coli pathogens	1122:1199	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	5	43	theme	components	778:787	arg1	rate					762:765	the rate	758:765	the rate of organic components	758:787	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	2	44	theme	monoliths	426:434	arg1	preparation					394:404	the preparation	390:404	the preparation of stable and rigid monoliths	390:434	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	7	45	dep	aureus	1163:1168	arg1	pathogens					1191:1199	pathogens	1191:1199	pathogens	1191:1199	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	8	46	theme	bone	1384:1387	arg1	infections					1389:1398	bone infections	1384:1398	bone infections	1384:1398	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	8	47	theme	local	1338:1342	arg1	release					1355:1361	local antibiotic release	1338:1361	local antibiotic release	1338:1361	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	7	48	theme	in	1045:1046	arg1	tests					1068:1072	in vitro antibacterial tests	1045:1072	in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP	1045:1113	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	0	49	theme	hydroxyapatite-based	36:55	arg1	composite					57:65	hydroxyapatite-based composite	36:65	hydroxyapatite-based composite	36:65	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	2	50	theme	rigid	420:424	arg1	monoliths					426:434	stable and rigid monoliths	409:434	stable and rigid monoliths	409:434	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	8	51	theme	showed	1219:1224	arg1	results					1226:1232	the showed results	1215:1232	the showed results	1215:1232	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	4	52	theme	biocomposite	595:606	arg1	strength					568:575	The compressive strength	552:575	The compressive strength of the fabricated biocomposite	552:606	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	0	53	theme	enhanced	72:79	arg1	strength					93:100	enhanced compressive strength	72:100	enhanced compressive strength	72:100	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	3	54	theme	composite	527:535	arg1	strength					500:507	the compressive strength	484:507	the compressive strength of the CIP-loaded composite	484:535	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	0	55	with	characterization	16:31	arg1	release					128:134	controlled antibiotic release	106:134	controlled antibiotic release	106:134	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	0	55	with	characterization	16:31	arg1	strength					93:100	enhanced compressive strength	72:100	enhanced compressive strength	72:100	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	1	56	theme	composite	139:147	arg1	based					149:153	A composite based	137:153	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP)	137:228	A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid-liquid mixing method.					
33811724	2	57	theme	stable	409:414	arg1	monoliths					426:434	stable and rigid monoliths	409:434	stable and rigid monoliths	409:434	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	8	58	theme	antibiotic	1344:1353	arg1	release					1355:1361	local antibiotic release	1338:1361	local antibiotic release	1338:1361	According to the showed results, the formulated composite with three-phase components could be a promising material for bone repair and local antibiotic release for the treatment of bone infections.					
33811724	3	59	from	effect	451:456	arg1	strength					500:507	the compressive strength	484:507	the compressive strength of the CIP-loaded composite	484:535	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	0	60	with	Formulation	0:10	arg1	release					128:134	controlled antibiotic release	106:134	controlled antibiotic release	106:134	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	0	60	with	Formulation	0:10	arg1	strength					93:100	enhanced compressive strength	72:100	enhanced compressive strength	72:100	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	2	61	theme	liquid	317:322	arg1	ratio					324:328	the liquid ratio	313:328	the liquid ratio	313:328	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	4	62	theme	compressive	556:566	arg1	strength					568:575	The compressive strength	552:575	The compressive strength of the fabricated biocomposite	552:606	The compressive strength of the fabricated biocomposite ranged in values from 1 to 6 MPa, comparable to those reported for cancellous bone.					
33811724	5	63	from	diminution	813:822	arg1	volume					874:879	the pore volume	865:879	the pore volume of the specimens	865:896	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	5	64	theme	surface	831:837	arg1	area					839:842	the surface area	827:842	the surface area	827:842	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	2	65	from	optimization	284:295	arg1	amount					365:370	a small amount	357:370	a small amount (≤5 wt%)	357:379	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	2	65	from	optimization	284:295	arg1	%					378:378	≤5 wt%	373:378	≤5 wt%	373:378	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	6	66	theme	release	931:937	arg1	experiments					939:949	the in vitro release experiments	918:949	the in vitro release experiments of the antibiotic	918:967	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	7	67	theme	aureus	1163:1168	arg1	inhibition					1134:1143	significant inhibition	1122:1143	significant inhibition of Staphylococcus aureus and Escherichia coli pathogens	1122:1199	Moreover, in vitro antibacterial tests performed on the biocomposite HA-CS5-CIP showed significant inhibition of Staphylococcus aureus and Escherichia coli pathogens.					
33811724	6	68	theme	CIP	1017:1019	arg1	release					1006:1012	a sustained and controlled release	979:1012	a sustained and controlled release of CIP	979:1019	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
33811724	5	69	theme	organic	770:776	arg1	components					778:787	organic components	770:787	organic components	770:787	The improvement of the mechanical properties with the increase of the rate of organic components was correlated with the diminution of the surface area and the reduction in the pore volume of the specimens.					
33811724	2	70	theme	chitosan	345:352	arg1	use					338:340	the use	334:340	the use of chitosan in a small amount (≤5 wt%)	334:379	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	2	70	theme	chitosan	345:352	arg1	optimization					284:295	The optimization	280:295	The optimization of the solid to the liquid ratio	280:328	The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths.					
33811724	0	71	theme	compressive	81:91	arg1	strength					93:100	enhanced compressive strength	72:100	enhanced compressive strength	72:100	Formulation and characterization of hydroxyapatite-based composite with enhanced compressive strength and controlled antibiotic release.					
33811724	3	72	theme	CIP	468:470	arg1	contents					472:479	CS and CIP contents	461:479	CS and CIP contents	461:479	A synergistic effect of CS and CIP contents on the compressive strength of the CIP-loaded composite was evidenced.					
33811724	6	73	theme	in	922:923	arg1	experiments					939:949	the in vitro release experiments	918:949	the in vitro release experiments of the antibiotic	918:967	On the other hand, the in vitro release experiments of the antibiotic indicated a sustained and controlled release of CIP over 10 days.					
32348857	6	0	theme	based	939:943	arg1	OCDDS					945:949	single pectin-Ca2+ based OCDDS	920:949	single pectin-Ca2+ based OCDDS	920:949	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	10	1	theme	carriers	1451:1458	arg1	biocompatibility					1417:1432	good biocompatibility	1412:1432	good biocompatibility of the composite carriers	1412:1458	The cytotoxicity assay indicated good biocompatibility of the composite carriers.					
32348857	6	2	theme	higher	883:888	arg1	efficiency					904:913	higher encapsulation efficiency	883:913	higher encapsulation efficiency	883:913	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	1	3	theme	nano-carbon	175:185	arg1	MNCS					195:198	MNCS	195:198	MNCS	195:198	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	3	theme	nano-carbon	175:185	arg1	sphere					187:192	The 3-aminopropyltriethoxysilane modified nano-carbon sphere	133:192	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS)	133:199	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	4	theme	release	259:265	arg1	properties					267:276	the controlled release properties	244:276	the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS)	244:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	8	5	theme	release	1173:1179	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	6	6	theme	pectin-Ca2+	927:937	arg1	OCDDS					945:949	single pectin-Ca2+ based OCDDS	920:949	single pectin-Ca2+ based OCDDS	920:949	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	10	7	theme	composite	1441:1449	arg1	carriers					1451:1458	the composite carriers	1437:1458	the composite carriers	1437:1458	The cytotoxicity assay indicated good biocompatibility of the composite carriers.					
32348857	10	8	theme	cytotoxicity	1383:1394	arg1	assay					1396:1400	The cytotoxicity assay	1379:1400	The cytotoxicity assay	1379:1400	The cytotoxicity assay indicated good biocompatibility of the composite carriers.					
32348857	4	9	theme	controlled	695:704	arg1	properties					714:723	the controlled release properties	691:723	the controlled release properties of the corresponding OCDDSs	691:751	The 5-fluorouracil (5-FU) was employed as the drug model and the controlled release properties of the corresponding OCDDSs were determined.					
32348857	0	10	theme	nano-carbon	89:99	arg1	sphere					101:106	the pectin/modified nano-carbon sphere	69:106	the pectin/modified nano-carbon sphere	69:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	6	11	theme	single	920:925	arg1	OCDDS					945:949	single pectin-Ca2+ based OCDDS	920:949	single pectin-Ca2+ based OCDDS	920:949	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	3	12	theme	pore	572:575	arg1	structure					577:585	the pore structure	568:585	the pore structure	568:585	The FE-SEM showed the pore structure when the MNCS was mingled with the pectin.					
32348857	0	13	theme	pectin/modified	73:87	arg1	sphere					101:106	the pectin/modified nano-carbon sphere	69:106	the pectin/modified nano-carbon sphere	69:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	2	14	dep	molecules	508:516	arg1	the					497:499	the	497:499	the	497:499	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	4	15	theme	release	706:712	arg1	properties					714:723	the controlled release properties	691:723	the controlled release properties of the corresponding OCDDSs	691:751	The 5-fluorouracil (5-FU) was employed as the drug model and the controlled release properties of the corresponding OCDDSs were determined.					
32348857	10	16	theme	good	1412:1415	arg1	biocompatibility					1417:1432	good biocompatibility	1412:1432	good biocompatibility of the composite carriers	1412:1458	The cytotoxicity assay indicated good biocompatibility of the composite carriers.					
32348857	7	17	from	films	1027:1031	arg1	OCDDSs					1005:1010	almost all the OCDDSs	990:1010	almost all the OCDDSs from composite films	990:1031	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	9	18	theme	kinetics	1318:1325	arg1	studies					1327:1333	the kinetics studies	1314:1333	the kinetics studies	1314:1333	In addition, the kinetics studies were performed to analyze the release data.					
32348857	2	19	theme	sphere	424:429	arg1	interaction					477:487	the electrostatic interaction	459:487	the electrostatic interaction between the pectin molecules and MNCS in the composite film	459:547	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	2	19	theme	sphere	424:429	arg1	modification					396:407	the successful modification	381:407	the successful modification of nano-carbon sphere via silylation reaction	381:453	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	8	20	theme	%	1194:1194	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	6	21	theme	encapsulation	890:902	arg1	efficiency					904:913	higher encapsulation efficiency	883:913	higher encapsulation efficiency	883:913	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	5	22	theme	encapsulation	788:800	arg1	efficiency					802:811	the encapsulation efficiency	784:811	the encapsulation efficiency	784:811	The values of the encapsulation efficiency ranged from 30.1% to 52.6%.					
32348857	7	23	theme	drug	956:959	arg1	release					961:967	The drug release	952:967	The drug release	952:967	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	2	24	theme	nano-carbon	412:422	arg1	sphere					424:429	nano-carbon sphere	412:429	nano-carbon sphere via silylation reaction	412:453	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	0	25	theme	novel	2:6	arg1	system					53:58	A novel and simple oral colon-specific drug delivery system	0:58	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere	0:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	2	26	from	molecules	508:516	arg1	film					544:547	the composite film	530:547	the composite film	530:547	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	8	27	theme	cumulative	1162:1171	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	28	theme	best	1128:1131	arg1	performance					1141:1151	best release performance	1128:1151	best release performance	1128:1151	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	29	theme	small	1247:1251	arg1	fluid					1264:1268	small intestinal fluid	1247:1268	small intestinal fluid	1247:1268	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	30	from	%	1202:1202	arg1	fluid					1264:1268	small intestinal fluid	1247:1268	small intestinal fluid	1247:1268	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	30	from	%	1202:1202	arg1	fluid					1280:1284	colon fluid	1274:1284	colon fluid	1274:1284	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	30	from	%	1202:1202	arg1	fluid					1240:1244	the simulated gastric fluid	1218:1244	the simulated gastric fluid	1218:1244	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	1	31	theme	pectin-based	285:296	arg1	system					332:337	the pectin-based oral colon-specific drug delivery system	281:337	the pectin-based oral colon-specific drug delivery system (OCDDS)	281:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	31	theme	pectin-based	285:296	arg1	OCDDS					340:344	OCDDS	340:344	OCDDS	340:344	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	7	32	theme	based	1093:1097	arg1	OCDDS					1099:1103	single pectin-Ca2+ based OCDDS	1074:1103	single pectin-Ca2+ based OCDDS	1074:1103	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	4	33	theme	OCDDSs	746:751	arg1	properties					714:723	the controlled release properties	691:723	the controlled release properties of the corresponding OCDDSs	691:751	The 5-fluorouracil (5-FU) was employed as the drug model and the controlled release properties of the corresponding OCDDSs were determined.					
32348857	8	34	theme	intestinal	1253:1262	arg1	fluid					1264:1268	small intestinal fluid	1247:1268	small intestinal fluid	1247:1268	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	2	35	from	MNCS	522:525	arg1	film					544:547	the composite film	530:547	the composite film	530:547	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	0	36	theme	simple	12:17	arg1	system					53:58	A novel and simple oral colon-specific drug delivery system	0:58	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere	0:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	1	37	theme	oral	298:301	arg1	system					332:337	the pectin-based oral colon-specific drug delivery system	281:337	the pectin-based oral colon-specific drug delivery system (OCDDS)	281:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	37	theme	oral	298:301	arg1	OCDDS					340:344	OCDDS	340:344	OCDDS	340:344	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	8	38	theme	sample	1110:1115	arg1	C					1117:1117	The sample C	1106:1117	The sample C	1106:1117	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	39	from	fluid	1280:1284	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	1	40	theme	colon-specific	303:316	arg1	system					332:337	the pectin-based oral colon-specific drug delivery system	281:337	the pectin-based oral colon-specific drug delivery system (OCDDS)	281:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	40	theme	colon-specific	303:316	arg1	OCDDS					340:344	OCDDS	340:344	OCDDS	340:344	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	0	41	theme	gel	122:124	arg1	films					126:130	gel films	122:130	gel films	122:130	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	8	42	from	rate	1181:1184	arg1	fluid					1264:1268	small intestinal fluid	1247:1268	small intestinal fluid	1247:1268	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	42	from	rate	1181:1184	arg1	fluid					1280:1284	colon fluid	1274:1284	colon fluid	1274:1284	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	42	from	rate	1181:1184	arg1	fluid					1240:1244	the simulated gastric fluid	1218:1244	the simulated gastric fluid	1218:1244	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	2	43	theme	successful	385:394	arg1	modification					396:407	the successful modification	381:407	the successful modification of nano-carbon sphere via silylation reaction	381:453	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	0	44	theme	colon-specific	24:37	arg1	system					53:58	A novel and simple oral colon-specific drug delivery system	0:58	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere	0:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	1	45	theme	drug	318:321	arg1	system					332:337	the pectin-based oral colon-specific drug delivery system	281:337	the pectin-based oral colon-specific drug delivery system (OCDDS)	281:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	45	theme	drug	318:321	arg1	OCDDS					340:344	OCDDS	340:344	OCDDS	340:344	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	7	46	theme	pectin-Ca2+	1081:1091	arg1	OCDDS					1099:1103	single pectin-Ca2+ based OCDDS	1074:1103	single pectin-Ca2+ based OCDDS	1074:1103	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	2	47	theme	silylation	435:444	arg1	reaction					446:453	silylation reaction	435:453	silylation reaction	435:453	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	4	48	theme	drug	676:679	arg1	5-fluorouracil					634:647	The 5-fluorouracil	630:647	The 5-fluorouracil (5-FU)	630:654	The 5-fluorouracil (5-FU) was employed as the drug model and the controlled release properties of the corresponding OCDDSs were determined.					
32348857	4	48	theme	drug	676:679	arg1	model					681:685	the drug model	672:685	the drug model	672:685	The 5-fluorouracil (5-FU) was employed as the drug model and the controlled release properties of the corresponding OCDDSs were determined.					
32348857	1	49	theme	pectin-Ca2+	216:226	arg1	film					228:231	pectin-Ca2+ film	216:231	pectin-Ca2+ film	216:231	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	0	50	theme	oral	19:22	arg1	system					53:58	A novel and simple oral colon-specific drug delivery system	0:58	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere	0:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	1	51	theme	delivery	323:330	arg1	system					332:337	the pectin-based oral colon-specific drug delivery system	281:337	the pectin-based oral colon-specific drug delivery system (OCDDS)	281:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	51	theme	delivery	323:330	arg1	OCDDS					340:344	OCDDS	340:344	OCDDS	340:344	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	5	52	theme	efficiency	802:811	arg1	values					774:779	The values	770:779	The values of the encapsulation efficiency	770:811	The values of the encapsulation efficiency ranged from 30.1% to 52.6%.					
32348857	6	53	theme	based	860:864	arg1	OCDDSs					866:871	All composite film based OCDDSs	841:871	All composite film based OCDDSs	841:871	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	2	54	theme	electrostatic	463:475	arg1	interaction					477:487	the electrostatic interaction	459:487	the electrostatic interaction between the pectin molecules and MNCS in the composite film	459:547	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	8	55	theme	%	1202:1202	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	0	56	theme	delivery	44:51	arg1	system					53:58	A novel and simple oral colon-specific drug delivery system	0:58	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere	0:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	1	57	theme	system	332:337	arg1	properties					267:276	the controlled release properties	244:276	the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS)	244:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	8	58	theme	release	1133:1139	arg1	performance					1141:1151	best release performance	1128:1151	best release performance	1128:1151	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	6	59	theme	film	855:858	arg1	OCDDSs					866:871	All composite film based OCDDSs	841:871	All composite film based OCDDSs	841:871	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	7	60	theme	better	1043:1048	arg1	properties					1058:1067	better release properties	1043:1067	better release properties	1043:1067	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	2	61	theme	FT-IR	352:356	arg1	measurements					358:369	The FT-IR measurements	348:369	The FT-IR measurements	348:369	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	0	62	theme	drug	39:42	arg1	system					53:58	A novel and simple oral colon-specific drug delivery system	0:58	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere	0:106	A novel and simple oral colon-specific drug delivery system based on the pectin/modified nano-carbon sphere nanocomposite gel films.					
32348857	7	63	theme	single	1074:1079	arg1	OCDDS					1099:1103	single pectin-Ca2+ based OCDDS	1074:1103	single pectin-Ca2+ based OCDDS	1074:1103	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	6	64	theme	composite	845:853	arg1	OCDDSs					866:871	All composite film based OCDDSs	841:871	All composite film based OCDDSs	841:871	All composite film based OCDDSs presented higher encapsulation efficiency than single pectin-Ca2+ based OCDDS.					
32348857	7	65	theme	composite	1017:1025	arg1	films					1027:1031	composite films	1017:1031	composite films	1017:1031	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	8	66	from	%	1213:1213	arg1	fluid					1264:1268	small intestinal fluid	1247:1268	small intestinal fluid	1247:1268	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	66	from	%	1213:1213	arg1	fluid					1280:1284	colon fluid	1274:1284	colon fluid	1274:1284	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	66	from	%	1213:1213	arg1	fluid					1240:1244	the simulated gastric fluid	1218:1244	the simulated gastric fluid	1218:1244	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	7	67	dep	studies	969:975	arg1	emerged					977:983	emerged	977:983	studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS	969:1103	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	9	68	theme	release	1365:1371	arg1	data					1373:1376	the release data	1361:1376	the release data	1361:1376	In addition, the kinetics studies were performed to analyze the release data.					
32348857	7	69	theme	release	1050:1056	arg1	properties					1058:1067	better release properties	1043:1067	better release properties	1043:1067	The drug release studies emerged that almost all the OCDDSs from composite films presented better release properties than single pectin-Ca2+ based OCDDS.					
32348857	8	70	from	%	1194:1194	arg1	fluid					1264:1268	small intestinal fluid	1247:1268	small intestinal fluid	1247:1268	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	70	from	%	1194:1194	arg1	fluid					1280:1284	colon fluid	1274:1284	colon fluid	1274:1284	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	70	from	%	1194:1194	arg1	fluid					1240:1244	the simulated gastric fluid	1218:1244	the simulated gastric fluid	1218:1244	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	4	71	theme	corresponding	732:744	arg1	OCDDSs					746:751	the corresponding OCDDSs	728:751	the corresponding OCDDSs	728:751	The 5-fluorouracil (5-FU) was employed as the drug model and the controlled release properties of the corresponding OCDDSs were determined.					
32348857	8	72	from	fluid	1240:1244	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	73	theme	simulated	1222:1230	arg1	fluid					1240:1244	the simulated gastric fluid	1218:1244	the simulated gastric fluid	1218:1244	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	74	theme	%	1213:1213	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	2	75	theme	composite	534:542	arg1	film					544:547	the composite film	530:547	the composite film	530:547	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	1	76	theme	3-aminopropyltriethoxysilane	137:164	arg1	MNCS					195:198	MNCS	195:198	MNCS	195:198	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	76	theme	3-aminopropyltriethoxysilane	137:164	arg1	sphere					187:192	The 3-aminopropyltriethoxysilane modified nano-carbon sphere	133:192	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS)	133:199	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	8	77	theme	gastric	1232:1238	arg1	fluid					1240:1244	the simulated gastric fluid	1218:1244	the simulated gastric fluid	1218:1244	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	2	78	theme	pectin	501:506	arg1	molecules					508:516	pectin molecules	501:516	pectin molecules	501:516	The FT-IR measurements indicated the successful modification of nano-carbon sphere via silylation reaction and the electrostatic interaction between the pectin molecules and MNCS in the composite film.					
32348857	8	79	theme	colon	1274:1278	arg1	fluid					1280:1284	colon fluid	1274:1284	colon fluid	1274:1284	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	8	80	from	fluid	1264:1268	arg1	rate					1181:1184	the cumulative release rate	1158:1184	the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively	1158:1298	The sample C revealed best release performance with the cumulative release rate of 32.17%, 22.77% and 63.89% in the simulated gastric fluid, small intestinal fluid and colon fluid, respectively.					
32348857	1	81	theme	modified	166:173	arg1	MNCS					195:198	MNCS	195:198	MNCS	195:198	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	81	theme	modified	166:173	arg1	sphere					187:192	The 3-aminopropyltriethoxysilane modified nano-carbon sphere	133:192	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS)	133:199	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
32348857	1	82	theme	controlled	248:257	arg1	properties					267:276	the controlled release properties	244:276	the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS)	244:345	The 3-aminopropyltriethoxysilane modified nano-carbon sphere (MNCS) was added into pectin-Ca2+ film to improve the controlled release properties of the pectin-based oral colon-specific drug delivery system (OCDDS).					
34601068	2	0	theme	nontoxic	592:599	arg1	hydrogel					549:556	F127 hydrogel	544:556	F127 hydrogel containing FMZC	544:572	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	2	0	theme	nontoxic	592:599	arg1	construct					601:609	a suitable and nontoxic construct	577:609	a suitable and nontoxic construct for encapsulation of MSCs	577:635	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	1	1	theme	regenerative	406:417	arg1	medicine					419:426	regenerative medicine	406:426	regenerative medicine	406:426	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	7	2	theme	skin	1521:1524	arg1	behavior					1531:1538	skin cell behavior	1521:1538	skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin	1521:1612	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	2	3	theme	suitable	579:586	arg1	hydrogel					549:556	F127 hydrogel	544:556	F127 hydrogel containing FMZC	544:572	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	2	3	theme	suitable	579:586	arg1	construct					601:609	a suitable and nontoxic construct	577:609	a suitable and nontoxic construct for encapsulation of MSCs	577:635	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	5	4	theme	bare	1242:1245	arg1	hydrogel					1247:1254	bare hydrogel	1242:1254	bare hydrogel	1242:1254	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	5	5	theme	Gram-negative	1214:1226	arg1	bacteria					1228:1235	both Gram-positive and Gram-negative bacteria	1191:1235	both Gram-positive and Gram-negative bacteria	1191:1235	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	7	6	theme	antibacterial	1482:1494	arg1	activity					1496:1503	the antibacterial activity	1478:1503	the antibacterial activity	1478:1503	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	2	7	theme	F127	544:547	arg1	construct					601:609	a suitable and nontoxic construct	577:609	a suitable and nontoxic construct for encapsulation of MSCs	577:635	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	2	7	theme	F127	544:547	arg1	hydrogel					549:556	F127 hydrogel	544:556	F127 hydrogel containing FMZC	544:572	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	0	8	theme	folic	133:137	arg1	microparticles					168:181	folic acid.MgO:ZnO/chitosan hybrid microparticles	133:181	folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	133:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	9	theme	delivery	385:392	arg1	hydrogel					260:267	biodegradable and thermosensitive F127 hydrogel	221:267	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	9	theme	delivery	385:392	arg1	vehicle					394:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle	350:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	7	10	theme	bacteria-accompanied	1666:1685	arg1	healing					1693:1699	bacteria-accompanied wound healing	1666:1699	bacteria-accompanied wound healing	1666:1699	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	0	11	theme	hybrid	161:166	arg1	microparticles					168:181	folic acid.MgO:ZnO/chitosan hybrid microparticles	133:181	folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	133:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	12	theme	medicine	419:426	arg1	purposes					446:453	regenerative medicine and wound healing purposes	406:453	regenerative medicine and wound healing purposes	406:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	2	13	theme	irradiation	673:683	arg1	presence					644:651	the presence	640:651	the presence of lysozyme and UVA irradiation	640:683	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	6	14	theme	MSC&FMZC	1322:1329	arg1	activity					1310:1317	the antibacterial activity	1292:1317	the antibacterial activity of MSC&FMZC	1292:1329	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	1	15	theme	wound	432:436	arg1	healing					438:444	wound healing	432:444	wound healing	432:444	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	4	16	theme	ZnO	938:940	arg1	nanocomposites					942:955	ZnO nanocomposites	938:955	ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects	938:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	5	17	theme	MSC&FMZC	1111:1118	arg1	hydrogel					1120:1127	lysozyme-treated MSC&FMZC hydrogel	1094:1127	lysozyme-treated MSC&FMZC hydrogel	1094:1127	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	3	18	theme	fibroblast	860:869	arg1	HFF					878:880	HFF	878:880	HFF	878:880	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	3	18	theme	fibroblast	860:869	arg1	cells					871:875	human foreskin fibroblast cells	845:875	human foreskin fibroblast cells (HFF)	845:881	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	2	19	theme	stem	699:702	arg1	cell					704:707	high stem cell viability and proliferation	694:735	cell	704:707	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	3	20	theme	higher	802:807	arg1	rate					823:826	a higher proliferation rate	800:826	a higher proliferation rate	800:826	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	0	21	theme	stem	87:90	arg1	cells					92:96	multiresponsive and thermosensitive stem cells	51:96	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	22	theme	mesenchymal	355:365	arg1	MSCs					379:382	MSCs	379:382	MSCs	379:382	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	22	theme	mesenchymal	355:365	arg1	cells					372:376	3D mesenchymal stem cells	352:376	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	3	23	theme	human	845:849	arg1	HFF					878:880	HFF	878:880	HFF	878:880	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	3	23	theme	human	845:849	arg1	cells					871:875	human foreskin fibroblast cells	845:875	human foreskin fibroblast cells (HFF)	845:881	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	0	24	theme	composite	98:106	arg1	hydrogel					113:120	composite F127 hydrogel	98:120	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	25	theme	cells	372:376	arg1	hydrogel					260:267	biodegradable and thermosensitive F127 hydrogel	221:267	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	25	theme	cells	372:376	arg1	vehicle					394:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle	350:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	2	26	dep	cell	704:707	arg1	viability					709:717	viability	709:717	viability	709:717	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	2	27	theme	MSCs	632:635	arg1	encapsulation					615:627	encapsulation	615:627	encapsulation of MSCs	615:635	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	1	28	theme	ZnO/chitosan	295:306	arg1	FMZC					326:329	FMZC	326:329	FMZC	326:329	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	28	theme	ZnO/chitosan	295:306	arg1	particles					315:323	ZnO/chitosan hybrid particles	295:323	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	0	29	theme	thermosensitive	71:85	arg1	cells					92:96	multiresponsive and thermosensitive stem cells	51:96	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	30	theme	hybrid	308:313	arg1	FMZC					326:329	FMZC	326:329	FMZC	326:329	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	30	theme	hybrid	308:313	arg1	particles					315:323	ZnO/chitosan hybrid particles	295:323	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	2	31	contain	containing	558:567	arg2	FMZC					569:572	FMZC	569:572	FMZC	569:572	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	2	31	contain	containing	558:567	arg1	construct					601:609	a suitable and nontoxic construct	577:609	a suitable and nontoxic construct for encapsulation of MSCs	577:635	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	2	31	contain	containing	558:567	arg1	hydrogel					549:556	F127 hydrogel	544:556	F127 hydrogel containing FMZC	544:572	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	5	32	theme	Gram-positive	1196:1208	arg1	bacteria					1228:1235	both Gram-positive and Gram-negative bacteria	1191:1235	both Gram-positive and Gram-negative bacteria	1191:1235	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	6	33	theme	F.MgO	1442:1446	arg1	activity					1430:1437	the photocatalytic activity	1411:1437	the photocatalytic activity of F.MgO:ZnO nanocomposites	1411:1465	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	0	34	theme	fibroblast	31:40	arg1	cells					42:46	fibroblast cells	31:46	fibroblast cells	31:46	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	4	35	theme	paracrine	990:998	arg1	factors					1000:1006	paracrine factors	990:1006	paracrine factors	990:1006	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	2	36	from	construct	601:609	arg1	presence					644:651	the presence	640:651	the presence of lysozyme and UVA irradiation	640:683	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	0	37	contain	containing	122:131	arg2	microparticles					168:181	folic acid.MgO:ZnO/chitosan hybrid microparticles	133:181	folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	133:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	0	37	contain	containing	122:131	arg1	hydrogel					113:120	composite F127 hydrogel	98:120	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	6	38	dep	F.MgO	1442:1446	arg1	nanocomposites					1452:1465	nanocomposites	1452:1465	nanocomposites	1452:1465	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	5	39	theme	anti-biofilm	1161:1172	arg1	activity					1174:1181	higher antibacterial and anti-biofilm activity	1136:1181	activity	1174:1181	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	7	40	theme	hydrogel	1552:1559	arg1	behavior					1531:1538	skin cell behavior	1521:1538	skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin	1521:1612	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	4	41	theme	synergistic	1052:1062	arg1	effects					1075:1081	synergistic biological effects	1052:1081	synergistic biological effects	1052:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	1	42	theme	thermosensitive	239:253	arg1	hydrogel					260:267	biodegradable and thermosensitive F127 hydrogel	221:267	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	42	theme	thermosensitive	239:253	arg1	vehicle					394:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle	350:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	7	43	theme	cell	1526:1529	arg1	behavior					1531:1538	skin cell behavior	1521:1538	skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin	1521:1612	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	1	44	theme	folic	280:284	arg1	acid.MgO					286:293	folic acid.MgO	280:293	folic acid.MgO	280:293	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	6	45	theme	antibacterial	1296:1308	arg1	activity					1310:1317	the antibacterial activity	1292:1317	the antibacterial activity of MSC&FMZC	1292:1329	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	4	46	from	microparticles	971:984	arg1	nanocomposites					942:955	ZnO nanocomposites	938:955	ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects	938:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	6	47	theme	significant	1268:1278	arg1	increase					1280:1287	a significant increase	1266:1287	a significant increase in the antibacterial activity of MSC&FMZC	1266:1329	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	7	48	theme	wound	1687:1691	arg1	healing					1693:1699	bacteria-accompanied wound healing	1666:1699	bacteria-accompanied wound healing	1666:1699	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	3	49	theme	final	742:746	arg1	MSC&FMZC					758:765	MSC&FMZC	758:765	MSC&FMZC	758:765	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	3	49	theme	final	742:746	arg1	hydrogel					748:755	The final hydrogel	738:755	The final hydrogel	738:755	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	0	50	theme	acid.MgO	139:146	arg1	microparticles					168:181	folic acid.MgO:ZnO/chitosan hybrid microparticles	133:181	folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	133:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	51	dep	hydrogel	260:267	arg1	FMZC					326:329	FMZC	326:329	FMZC	326:329	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	51	dep	hydrogel	260:267	arg1	particles					315:323	ZnO/chitosan hybrid particles	295:323	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	6	52	theme	treated	1351:1357	arg1	bacteria					1359:1366	the treated bacteria	1347:1366	the treated bacteria	1347:1366	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	1	53	contain	containing	269:278	arg1	hydrogel					260:267	biodegradable and thermosensitive F127 hydrogel	221:267	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	53	contain	containing	269:278	arg2	acid.MgO					286:293	folic acid.MgO	280:293	folic acid.MgO	280:293	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	53	contain	containing	269:278	arg1	vehicle					394:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle	350:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	54	theme	biodegradable	221:233	arg1	hydrogel					260:267	biodegradable and thermosensitive F127 hydrogel	221:267	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	54	theme	biodegradable	221:233	arg1	vehicle					394:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle	350:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	7	55	theme	promising	1643:1651	arg1	scaffold					1653:1660	a promising scaffold	1641:1660	a promising scaffold for bacteria-accompanied wound healing	1641:1699	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	7	55	theme	promising	1643:1651	arg1	it					1615:1616	it	1615:1616	it	1615:1616	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	2	56	theme	high	694:697	arg1	cell					704:707	high stem cell viability and proliferation	694:735	cell	704:707	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	4	57	dep	attributed	905:914	arg1	nanocomposites					942:955	ZnO nanocomposites	938:955	ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects	938:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	3	58	dep	lysozyme	783:790	arg1	response					771:778	response	771:778	response	771:778	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	6	59	from	increase	1280:1287	arg1	activity					1310:1317	the antibacterial activity	1292:1317	the antibacterial activity of MSC&FMZC	1292:1329	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	0	60	theme	skin	187:190	arg1	regeneration					192:203	skin regeneration	187:203	skin regeneration	187:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	6	61	theme	photocatalytic	1415:1428	arg1	activity					1430:1437	the photocatalytic activity	1411:1437	the photocatalytic activity of F.MgO:ZnO nanocomposites	1411:1465	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	1	62	theme	healing	438:444	arg1	purposes					446:453	regenerative medicine and wound healing purposes	406:453	regenerative medicine and wound healing purposes	406:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	6	63	theme	UVA	1386:1388	arg1	irradiation					1390:1400	UVA irradiation	1386:1400	UVA irradiation	1386:1400	However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites.					
34601068	1	64	theme	3D	352:353	arg1	MSCs					379:382	MSCs	379:382	MSCs	379:382	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	64	theme	3D	352:353	arg1	cells					372:376	3D mesenchymal stem cells	352:376	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	7	65	theme	skin	1609:1612	arg1	activities					1595:1604	the regenerative activities	1578:1604	the regenerative activities of skin	1578:1612	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	3	66	theme	proliferation	809:821	arg1	rate					823:826	a higher proliferation rate	800:826	a higher proliferation rate	800:826	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	0	67	theme	F127	108:111	arg1	hydrogel					113:120	composite F127 hydrogel	98:120	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	68	theme	stem	367:370	arg1	MSCs					379:382	MSCs	379:382	MSCs	379:382	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	68	theme	stem	367:370	arg1	cells					372:376	3D mesenchymal stem cells	352:376	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	3	69	theme	foreskin	851:858	arg1	HFF					878:880	HFF	878:880	HFF	878:880	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	3	69	theme	foreskin	851:858	arg1	cells					871:875	human foreskin fibroblast cells	845:875	human foreskin fibroblast cells (HFF)	845:881	The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF).					
34601068	4	70	theme	released	923:930	arg1	F.MgO					932:936	the released F.MgO	919:936	the released F.MgO	919:936	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	5	71	dep	antibacterial	1143:1155	arg1	higher					1136:1141	higher	1136:1141	higher	1136:1141	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	2	72	theme	UVA	669:671	arg1	irradiation					673:683	UVA irradiation	669:683	UVA irradiation	669:683	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	5	73	theme	lysozyme-treated	1094:1109	arg1	hydrogel					1120:1127	lysozyme-treated MSC&FMZC hydrogel	1094:1127	lysozyme-treated MSC&FMZC hydrogel	1094:1127	Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel.					
34601068	0	74	dep	cells	92:96	arg1	hydrogel					113:120	composite F127 hydrogel	98:120	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	0	75	theme	ZnO/chitosan	148:159	arg1	microparticles					168:181	folic acid.MgO:ZnO/chitosan hybrid microparticles	133:181	folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	133:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	2	76	theme	lysozyme	656:663	arg1	presence					644:651	the presence	640:651	the presence of lysozyme and UVA irradiation	640:683	The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation.					
34601068	4	77	from	MSCs	1013:1016	arg1	nanocomposites					942:955	ZnO nanocomposites	938:955	ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects	938:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	0	78	theme	cells	42:46	arg1	proliferation					14:26	the proliferation	10:26	the proliferation of fibroblast cells	10:46	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	4	79	from	factors	1000:1006	arg1	nanocomposites					942:955	ZnO nanocomposites	938:955	ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects	938:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	7	80	theme	regenerative	1582:1593	arg1	activities					1595:1604	the regenerative activities	1578:1604	the regenerative activities of skin	1578:1612	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	4	81	theme	chitosan	962:969	arg1	microparticles					971:984	chitosan microparticles	962:984	chitosan microparticles	962:984	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
34601068	0	82	theme	multiresponsive	51:65	arg1	cells					92:96	multiresponsive and thermosensitive stem cells	51:96	multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration	51:203	Tailoring the proliferation of fibroblast cells by multiresponsive and thermosensitive stem cells composite F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid microparticles for skin regeneration.					
34601068	1	83	theme	UVA	503:505	arg1	irradiation					507:517	UVA irradiation	503:517	UVA irradiation	503:517	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	84	theme	F127	255:258	arg1	hydrogel					260:267	biodegradable and thermosensitive F127 hydrogel	221:267	biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC)	221:330	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	1	84	theme	F127	255:258	arg1	vehicle					394:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle	350:400	a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes	350:453	In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation.					
34601068	7	85	theme	MSC&FMZC	1543:1550	arg1	hydrogel					1552:1559	MSC&FMZC hydrogel	1543:1559	MSC&FMZC hydrogel	1543:1559	Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.					
34601068	4	86	theme	biological	1064:1073	arg1	effects					1075:1081	synergistic biological effects	1052:1081	synergistic biological effects	1052:1081	These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects.					
32745549	0	0	theme	amine	85:89	arg1	SBA					100:102	amine modified SBA 15	85:105	amine modified SBA 15 with excellent photostability and biocompatibility	85:156	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	3	1	theme	SA-CIS/ZnS	708:717	arg1	QDs					719:721	the SA-CIS/ZnS QDs	704:721	the SA-CIS/ZnS QDs	704:721	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	6	2	theme	QDs-silica	1108:1117	arg1	composite					1120:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	2	3	theme	infrared	475:482	arg1	emission					484:491	near infrared emission	470:491	near infrared emission	470:491	The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation.					
32745549	4	4	theme	volume	858:863	arg1	analysis					865:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	3	5	with	loading	691:697	arg1	QDs					719:721	the SA-CIS/ZnS QDs	704:721	the SA-CIS/ZnS QDs	704:721	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	6	theme	mesoporous	637:646	arg1	nature					648:653	the mesoporous nature	633:653	the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs	633:721	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	0	7	theme	SBA	100:102	arg1	channels					73:80	the mesoporous channels	58:80	the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility	58:156	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	1	8	theme	mesoporous	322:331	arg1	channels					333:340	mesoporous channels	322:340	mesoporous channels	322:340	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	3	9	theme	Transmission	545:556	arg1	TEM					579:581	TEM	579:581	TEM	579:581	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	9	theme	Transmission	545:556	arg1	microscopy					567:576	Transmission electron microscopy	545:576	Transmission electron microscopy (TEM)	545:582	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	1	10	theme	greener	239:245	arg1	method					247:252	a greener method	237:252	a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility	237:426	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	0	11	theme	modified	91:98	arg1	SBA					100:102	amine modified SBA 15	85:105	amine modified SBA 15 with excellent photostability and biocompatibility	85:156	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	4	12	theme	SA-CIS/ZnS	755:764	arg1	QDs					766:768	SA-CIS/ZnS QDs	755:768	SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix	755:805	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	3	13	theme	electron	558:565	arg1	TEM					579:581	TEM	579:581	TEM	579:581	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	13	theme	electron	558:565	arg1	microscopy					567:576	Transmission electron microscopy	545:576	Transmission electron microscopy (TEM)	545:582	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	4	14	theme	infrared	964:971	arg1	spectroscopy					980:991	infrared (FTIR) spectroscopy	964:991	infrared (FTIR) spectroscopy	964:991	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	4	15	theme	BET	848:850	arg1	analysis					865:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	6	16	theme	SA-CIS/ZnS-SBA15-NH2	1086:1105	arg1	composite					1120:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	2	17	theme	SA	504:505	arg1	passivation					507:517	SA passivation	504:517	SA passivation	504:517	The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation.					
32745549	1	18	theme	amine	345:349	arg1	SBA15-NH2					368:376	SBA15-NH2	368:376	SBA15-NH2	368:376	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	1	18	theme	amine	345:349	arg1	silica					360:365	amine modified silica	345:365	amine modified silica (SBA15-NH2)	345:377	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	6	19	theme	resultant	1076:1084	arg1	composite					1120:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	2	20	theme	as-synthesized	433:446	arg1	QDs					456:458	The as-synthesized CIS/ZnS QDs	429:458	The as-synthesized CIS/ZnS QDs	429:458	The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation.					
32745549	1	21	theme	modified	351:358	arg1	SBA15-NH2					368:376	SBA15-NH2	368:376	SBA15-NH2	368:376	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	1	21	theme	modified	351:358	arg1	silica					360:365	amine modified silica	345:365	amine modified silica (SBA15-NH2)	345:377	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	4	22	theme	pore	853:856	arg1	analysis					865:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	4	23	theme	effective	728:736	arg1	encapsulation					738:750	The effective encapsulation	724:750	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix	724:805	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	0	24	theme	Sodium	0:5	arg1	alginate					7:14	Sodium alginate	0:14	Sodium alginate	0:14	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	6	25	theme	theranostic	1262:1272	arg1	applications					1274:1285	theranostic applications	1262:1285	theranostic applications	1262:1285	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	6	26	theme	versatile	1239:1247	arg1	material					1249:1256	a versatile material	1237:1256	a versatile material for theranostic applications	1237:1285	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	0	27	with	SBA	100:102	arg1	biocompatibility					141:156	biocompatibility	141:156	biocompatibility	141:156	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	0	27	with	SBA	100:102	arg1	photostability					122:135	excellent photostability	112:135	excellent photostability	112:135	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	1	28	dep	report	169:174	arg1	QDs					228:230	QDs	228:230	QDs	228:230	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	1	29	theme	CuInS2/ZnS	193:202	arg1	dots					222:225	CuInS2/ZnS (CIS/ZnS) quantum dots	193:225	CuInS2/ZnS (CIS/ZnS) quantum dots	193:225	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	0	30	theme	CuInS2/ZnS	27:36	arg1	QDs					38:40	CuInS2/ZnS QDs	27:40	CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility	27:156	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	2	31	dep	passivation	507:517	arg1	encapsulation					530:542	encapsulation	530:542	encapsulation	530:542	The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation.					
32745549	1	32	theme	sodium	266:271	arg1	passivation					287:297	sodium alginate (SA) passivation	266:297	sodium alginate (SA) passivation	266:297	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	0	33	theme	excellent	112:120	arg1	photostability					122:135	excellent photostability	112:135	excellent photostability	112:135	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	1	34	theme	silica	360:365	arg1	encapsulation					303:315	encapsulation	303:315	encapsulation into mesoporous channels of amine modified silica (SBA15-NH2)	303:377	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	1	34	theme	silica	360:365	arg1	passivation					287:297	sodium alginate (SA) passivation	266:297	sodium alginate (SA) passivation	266:297	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	6	35	theme	lung	1176:1179	arg1	A549					1189:1192	A549	1189:1192	A549	1189:1192	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	6	35	theme	lung	1176:1179	arg1	cancer					1181:1186	lung cancer	1176:1186	lung cancer (A549)	1176:1193	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	1	36	theme	CIS/ZnS	205:211	arg1	dots					222:225	CuInS2/ZnS (CIS/ZnS) quantum dots	193:225	CuInS2/ZnS (CIS/ZnS) quantum dots	193:225	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	2	37	theme	near	470:473	arg1	emission					484:491	near infrared emission	470:491	near infrared emission	470:491	The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation.					
32745549	1	38	theme	alginate	273:280	arg1	passivation					287:297	sodium alginate (SA) passivation	266:297	sodium alginate (SA) passivation	266:297	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	4	39	dep	Fourier	946:952	arg1	transform					954:962	transform	954:962	transform infrared (FTIR) spectroscopy	954:991	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	3	40	theme	angle	594:598	arg1	XRD					619:621	XRD	619:621	XRD	619:621	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	40	theme	angle	594:598	arg1	diffraction					606:616	Small angle X-ray diffraction	588:616	Small angle X-ray diffraction (XRD)	588:622	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	41	theme	X-ray	600:604	arg1	XRD					619:621	XRD	619:621	XRD	619:621	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	41	theme	X-ray	600:604	arg1	diffraction					606:616	Small angle X-ray diffraction	588:616	Small angle X-ray diffraction (XRD)	588:622	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	1	42	theme	quantum	214:220	arg1	dots					222:225	CuInS2/ZnS (CIS/ZnS) quantum dots	193:225	CuInS2/ZnS (CIS/ZnS) quantum dots	193:225	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	1	43	theme	SA	283:284	arg1	passivation					287:297	sodium alginate (SA) passivation	266:297	sodium alginate (SA) passivation	266:297	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	4	44	theme	Brunauer-Emmett-Teller	824:845	arg1	analysis					865:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	Brunauer-Emmett-Teller (BET) pore volume analysis	824:872	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	2	45	theme	CIS/ZnS	448:454	arg1	QDs					456:458	The as-synthesized CIS/ZnS QDs	429:458	The as-synthesized CIS/ZnS QDs	429:458	The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation.					
32745549	6	46	theme	kidney	1199:1204	arg1	lines					1221:1225	kidney (HEK 293) cell lines	1199:1225	kidney (HEK 293) cell lines	1199:1225	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	4	47	dep	infrared	964:971	arg1	FTIR					974:977	FTIR	974:977	FTIR	974:977	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	3	48	theme	SBA-15	662:667	arg1	nature					648:653	the mesoporous nature	633:653	the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs	633:721	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	49	theme	Small	588:592	arg1	XRD					619:621	XRD	619:621	XRD	619:621	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	3	49	theme	Small	588:592	arg1	diffraction					606:616	Small angle X-ray diffraction	588:616	Small angle X-ray diffraction (XRD)	588:622	Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs.					
32745549	1	50	theme	improved	383:390	arg1	photostability					392:405	improved photostability	383:405	improved photostability	383:405	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	6	51	theme	cell	1216:1219	arg1	lines					1221:1225	kidney (HEK 293) cell lines	1199:1225	kidney (HEK 293) cell lines	1199:1225	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	5	52	theme	QDs	1020:1022	arg1	photostability					998:1011	The photostability	994:1011	The photostability of the QDs	994:1022	The photostability of the QDs was greatly enhanced after these modifications.					
32745549	6	53	theme	remarkable	1140:1149	arg1	biocompatibility					1151:1166	remarkable biocompatibility	1140:1166	remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines	1140:1225	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	1	54	theme	dots	222:225	arg1	synthesis					180:188	the synthesis	176:188	the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots	176:225	We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility.					
32745549	6	55	contain	possessed	1130:1138	arg2	biocompatibility					1151:1166	remarkable biocompatibility	1140:1166	remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines	1140:1225	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	6	55	contain	possessed	1130:1138	arg1	composite					1120:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite	1072:1128	The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.					
32745549	4	56	theme	QDs	766:768	arg1	encapsulation					738:750	The effective encapsulation	724:750	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix	724:805	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	4	57	theme	matrix	800:805	arg1	pores					781:785	the pores	777:785	the pores of SBA15-NH2 matrix	777:805	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32745549	0	58	theme	mesoporous	62:71	arg1	channels					73:80	the mesoporous channels	58:80	the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility	58:156	Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility.					
32745549	4	59	theme	SBA15-NH2	790:798	arg1	matrix					800:805	SBA15-NH2 matrix	790:805	SBA15-NH2 matrix	790:805	The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy.					
32703583	14	0	theme	limb	1565:1568	arg1	ischemia					1570:1577	acute limb ischemia	1559:1577	acute limb ischemia that required revascularization	1559:1609	Three patients (0.3%) had acute limb ischemia that required revascularization.					
32703583	1	1	theme	percutaneous	263:274	arg1	PCI					299:301	PCI	299:301	PCI	299:301	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	1	theme	percutaneous	263:274	arg1	intervention					285:296	percutaneous coronary intervention	263:296	percutaneous coronary intervention (PCI)	263:302	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	15	2	theme	negative	1735:1742	arg1	patients					1744:1751	biomarker negative patients	1725:1751	biomarker negative patients who undergo transfemoral PCI	1725:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	15	3	theme	ischemic	1686:1693	arg1	complications					1708:1720	low ischemic and bleeding complications	1682:1720	low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI	1682:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	6	4	dep	METHODS	761:767	arg1	January					774:780	January	774:780	January	774:780	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	1	5	theme	low-dose	179:186	arg1	heparin					188:194	low-dose heparin	179:194	low-dose heparin (40 IU/kg)	179:205	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	5	theme	low-dose	179:186	arg1	40 IU/kg					197:204	40 IU/kg	197:204	40 IU/kg	197:204	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	6	6	theme	initial	867:873	arg1	bolus					875:879	an initial bolus	864:879	an initial bolus of 40 IU/kg of heparin	864:902	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	6	7	dep	January	774:780	arg1	February					790:797	February	790:797	February	790:797	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	6	7	dep	January	774:780	arg1	to					787:788	to	787:788	to	787:788	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	5	8	theme	activating	633:642	arg1	times					653:657	supratherapeutic activating clotting times	616:657	supratherapeutic activating clotting times (ACT)	616:663	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	9	9	theme	bleeding	1184:1191	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	9	theme	bleeding	1184:1191	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	9	theme	bleeding	1184:1191	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	9	theme	bleeding	1184:1191	arg1	bleeding					1184:1191	bleeding	1184:1191	bleeding	1184:1191	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	9	theme	bleeding	1184:1191	arg1	point					1046:1050	The primary end point	1030:1050	The primary end point	1030:1050	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	9	theme	bleeding	1184:1191	arg1	composite					1060:1068	the composite	1056:1068	the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days	1056:1206	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	8	10	theme	antiplatelet	1008:1019	arg1	therapy					1021:1027	dual antiplatelet therapy	1003:1027	dual antiplatelet therapy	1003:1027	Patients were routinely pretreated with dual antiplatelet therapy.					
32703583	11	11	theme	end	1336:1338	arg1	point					1340:1344	the primary end point	1324:1344	the primary end point	1324:1344	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	12	theme	cardiac	1364:1370	arg1	death					1372:1376	cardiac death	1364:1376	cardiac death in 1.0%	1364:1384	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	14	13	theme	acute	1559:1563	arg1	ischemia					1570:1577	acute limb ischemia	1559:1577	acute limb ischemia that required revascularization	1559:1609	Three patients (0.3%) had acute limb ischemia that required revascularization.					
32703583	15	14	theme	low-dose	1646:1653	arg1	heparin					1655:1661	low-dose heparin	1646:1661	low-dose heparin	1646:1661	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	1	15	dep	OBJECTIVES	109:118	arg1	efficacy					135:142	efficacy	135:142	efficacy	135:142	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	15	dep	OBJECTIVES	109:118	arg1	safety					124:129	safety	124:129	safety	124:129	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	15	dep	OBJECTIVES	109:118	arg1	OBJECTIVES					109:118	OBJECTIVES The safety and efficacy	109:142	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg)	109:205	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	11	16	theme	urgent	1399:1404	arg1	TVR					1406:1408	urgent TVR	1399:1408	urgent TVR in 0.7%	1399:1416	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	0	17	theme	Low-Dose	60:67	arg1	Heparin					69:75	Low-Dose Heparin	60:75	Low-Dose Heparin	60:75	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	1	18	theme	initial	150:156	arg1	bolus					170:174	an initial intravenous bolus	147:174	an initial intravenous bolus of low-dose heparin (40 IU/kg)	147:205	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	0	19	from	Intervention	22:33	arg1	Patients					99:106	Biomarker-Negative Patients	80:106	Biomarker-Negative Patients	80:106	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	10	20	theme	initial	1231:1237	arg1	time					1264:1267	The initial mean activating clotting time	1227:1267	The initial mean activating clotting time	1227:1267	RESULTS The initial mean activating clotting time was 235.4 ± 26.6 s.					
32703583	5	21	theme	heparin	579:585	arg1	bolus					561:565	An initial bolus	550:565	An initial bolus of low-dose heparin	550:585	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	5	21	theme	heparin	579:585	arg1	advantageous					594:605	advantageous	594:605	advantageous	594:605	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	1	22	theme	bolus	170:174	arg1	efficacy					135:142	efficacy	135:142	efficacy	135:142	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	22	theme	bolus	170:174	arg1	safety					124:129	safety	124:129	safety	124:129	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	22	theme	bolus	170:174	arg1	OBJECTIVES					109:118	OBJECTIVES The safety and efficacy	109:142	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg)	109:205	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	15	23	theme	low	1682:1684	arg1	complications					1708:1720	low ischemic and bleeding complications	1682:1720	low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI	1682:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	5	24	theme	heparin	723:729	arg1	administration					694:707	the administration	690:707	the administration of additional heparin if the ACT is subtherapeutic	690:758	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	1	25	dep	safety	124:129	arg1	The					120:122	The	120:122	The	120:122	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	9	26	theme	cardiac	1073:1079	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	5	27	theme	initial	553:559	arg1	bolus					561:565	An initial bolus	550:565	An initial bolus of low-dose heparin	550:585	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	5	27	theme	initial	553:559	arg1	advantageous					594:605	advantageous	594:605	advantageous	594:605	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	10	28	theme	activating	1244:1253	arg1	time					1264:1267	The initial mean activating clotting time	1227:1267	The initial mean activating clotting time	1227:1267	RESULTS The initial mean activating clotting time was 235.4 ± 26.6 s.					
32703583	0	29	theme	Percutaneous	0:11	arg1	Intervention					22:33	Percutaneous Coronary Intervention	0:33	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients	0:106	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	15	30	dep	CONCLUSION	1612:1621	arg1	associated					1666:1675	associated	1666:1675	is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI	1663:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	9	31	theme	infarction	1099:1108	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	31	theme	infarction	1099:1108	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	31	theme	infarction	1099:1108	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	31	theme	infarction	1099:1108	arg1	bleeding					1184:1191	bleeding	1184:1191	bleeding	1184:1191	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	31	theme	infarction	1099:1108	arg1	point					1046:1050	The primary end point	1030:1050	The primary end point	1030:1050	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	31	theme	infarction	1099:1108	arg1	composite					1060:1068	the composite	1056:1068	the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days	1056:1206	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	3	32	theme	ideal	418:422	arg1	dose					424:427	the ideal dose	414:427	the ideal dose of heparin	414:438	However, the ideal dose of heparin has not been evaluated in a randomized trial.					
32703583	9	33	theme	end	1042:1044	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	33	theme	end	1042:1044	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	33	theme	end	1042:1044	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	33	theme	end	1042:1044	arg1	bleeding					1184:1191	bleeding	1184:1191	bleeding	1184:1191	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	33	theme	end	1042:1044	arg1	point					1046:1050	The primary end point	1030:1050	The primary end point	1030:1050	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	33	theme	end	1042:1044	arg1	composite					1060:1068	the composite	1056:1068	the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days	1056:1206	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	0	34	with	Intervention	22:33	arg1	Bolus					51:55	an Initial Bolus	40:55	an Initial Bolus of Low-Dose Heparin	40:75	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	6	35	theme	elective	829:836	arg1	PCI					851:853	elective transfemoral PCI	829:853	elective transfemoral PCI	829:853	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	9	36	theme	target	1123:1128	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	36	theme	target	1123:1128	arg1	TVR					1156:1158	TVR	1156:1158	TVR	1156:1158	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	3	37	theme	randomized	468:477	arg1	trial					479:483	a randomized trial	466:483	a randomized trial	466:483	However, the ideal dose of heparin has not been evaluated in a randomized trial.					
32703583	9	38	theme	revascularization	1137:1153	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	38	theme	revascularization	1137:1153	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	38	theme	revascularization	1137:1153	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	38	theme	revascularization	1137:1153	arg1	bleeding					1184:1191	bleeding	1184:1191	bleeding	1184:1191	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	38	theme	revascularization	1137:1153	arg1	point					1046:1050	The primary end point	1030:1050	The primary end point	1030:1050	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	38	theme	revascularization	1137:1153	arg1	composite					1060:1068	the composite	1056:1068	the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days	1056:1206	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	1	39	theme	negative	234:241	arg1	patients					243:250	biomarker negative patients	224:250	biomarker negative patients undergoing percutaneous coronary intervention (PCI)	224:302	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	5	40	theme	clotting	644:651	arg1	times					653:657	supratherapeutic activating clotting times	616:657	supratherapeutic activating clotting times (ACT)	616:663	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	4	41	theme	higher	490:495	arg1	dose					497:500	The higher dose	486:500	The higher dose of 100 IU/kg	486:513	The higher dose of 100 IU/kg may increase the risk of bleeding.					
32703583	6	42	theme	heparin	896:902	arg1	40 IU/kg					884:891	40 IU/kg	884:891	40 IU/kg of heparin	884:902	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	15	43	theme	biomarker	1725:1733	arg1	patients					1744:1751	biomarker negative patients	1725:1751	biomarker negative patients who undergo transfemoral PCI	1725:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	12	44	dep	uncommon	1467:1474	arg1	%					1480:1480	0.2%	1477:1480	0.2%	1477:1480	Stent thrombosis was uncommon (0.2%).					
32703583	1	45	theme	coronary	276:283	arg1	PCI					299:301	PCI	299:301	PCI	299:301	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	1	45	theme	coronary	276:283	arg1	intervention					285:296	percutaneous coronary intervention	263:296	percutaneous coronary intervention (PCI)	263:302	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	6	46	theme	40 IU/kg	884:891	arg1	bolus					875:879	an initial bolus	864:879	an initial bolus of 40 IU/kg of heparin	864:902	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	13	47	theme	No	1484:1485	arg1	patients					1487:1494	No patients	1484:1494	No patients	1484:1494	No patients developed profound thrombocytopenia.					
32703583	8	48	theme	dual	1003:1006	arg1	therapy					1021:1027	dual antiplatelet therapy	1003:1027	dual antiplatelet therapy	1003:1027	Patients were routinely pretreated with dual antiplatelet therapy.					
32703583	5	49	theme	supratherapeutic	616:631	arg1	times					653:657	supratherapeutic activating clotting times	616:657	supratherapeutic activating clotting times (ACT)	616:663	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	1	50	theme	heparin	188:194	arg1	bolus					170:174	an initial intravenous bolus	147:174	an initial intravenous bolus of low-dose heparin (40 IU/kg)	147:205	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	11	51	theme	event	1302:1306	arg1	low					1319:1321	low	1319:1321	low	1319:1321	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	51	theme	event	1302:1306	arg1	rates					1308:1312	The clinical event rates	1289:1312	The clinical event rates	1289:1312	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	52	from	%	1443:1443	arg1	bleeding					1428:1435	bleeding	1428:1435	bleeding	1428:1435	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	53	theme	primary	1328:1334	arg1	point					1340:1344	the primary end point	1324:1344	the primary end point	1324:1344	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	54	from	death	1372:1376	arg1	%					1384:1384	1.0%	1381:1384	1.0%	1381:1384	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	54	from	death	1372:1376	arg1	%					1416:1416	0.7%	1413:1416	0.7%	1413:1416	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	54	from	death	1372:1376	arg1	%					1396:1396	3.1%	1393:1396	3.1%	1393:1396	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	5	55	dep	the	734:736	arg1	ACT					738:740	ACT	738:740	ACT	738:740	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	2	56	dep	BACKGROUND	305:314	arg1	recommended					364:374	recommended	364:374	is currently recommended for patients undergoing PCI	351:402	BACKGROUND A bolus of 70-100 IU/kg of heparin is currently recommended for patients undergoing PCI.					
32703583	12	57	theme	Stent	1446:1450	arg1	thrombosis					1452:1461	Stent thrombosis	1446:1461	Stent thrombosis	1446:1461	Stent thrombosis was uncommon (0.2%).					
32703583	5	58	dep	heparin	723:729	arg1	subtherapeutic					745:758	subtherapeutic	745:758	subtherapeutic	745:758	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	9	59	theme	primary	1034:1040	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	59	theme	primary	1034:1040	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	59	theme	primary	1034:1040	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	59	theme	primary	1034:1040	arg1	bleeding					1184:1191	bleeding	1184:1191	bleeding	1184:1191	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	59	theme	primary	1034:1040	arg1	point					1046:1050	The primary end point	1030:1050	The primary end point	1030:1050	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	59	theme	primary	1034:1040	arg1	composite					1060:1068	the composite	1056:1068	the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days	1056:1206	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	0	60	theme	Heparin	69:75	arg1	Bolus					51:55	an Initial Bolus	40:55	an Initial Bolus of Low-Dose Heparin	40:75	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	14	61	contain	had	1555:1557	arg1	patients					1539:1546	Three patients	1533:1546	Three patients (0.3%)	1533:1553	Three patients (0.3%) had acute limb ischemia that required revascularization.					
32703583	14	61	contain	had	1555:1557	arg2	ischemia					1570:1577	acute limb ischemia	1559:1577	acute limb ischemia that required revascularization	1559:1609	Three patients (0.3%) had acute limb ischemia that required revascularization.					
32703583	14	61	contain	had	1555:1557	arg1	%					1552:1552	0.3%	1549:1552	0.3%	1549:1552	Three patients (0.3%) had acute limb ischemia that required revascularization.					
32703583	11	62	from	bleeding	1428:1435	arg1	%					1443:1443	1.9%	1440:1443	1.9%	1440:1443	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	10	63	theme	mean	1239:1242	arg1	time					1264:1267	The initial mean activating clotting time	1227:1267	The initial mean activating clotting time	1227:1267	RESULTS The initial mean activating clotting time was 235.4 ± 26.6 s.					
32703583	0	64	theme	Biomarker-Negative	80:97	arg1	Patients					99:106	Biomarker-Negative Patients	80:106	Biomarker-Negative Patients	80:106	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	1	65	theme	intravenous	158:168	arg1	bolus					170:174	an initial intravenous bolus	147:174	an initial intravenous bolus of low-dose heparin (40 IU/kg)	147:205	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	4	66	theme	100 IU/kg	505:513	arg1	dose					497:500	The higher dose	486:500	The higher dose of 100 IU/kg	486:513	The higher dose of 100 IU/kg may increase the risk of bleeding.					
32703583	5	67	theme	additional	712:721	arg1	heparin					723:729	additional heparin	712:729	additional heparin if the ACT is subtherapeutic	712:758	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	10	68	dep	RESULTS	1219:1225	arg1	235.4 ± 26.6 s					1273:1286	235.4 ± 26.6 s	1273:1286	was 235.4 ± 26.6 s	1269:1286	RESULTS The initial mean activating clotting time was 235.4 ± 26.6 s.					
32703583	11	69	theme	clinical	1293:1300	arg1	low					1319:1321	low	1319:1321	low	1319:1321	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	69	theme	clinical	1293:1300	arg1	rates					1308:1312	The clinical event rates	1289:1312	The clinical event rates	1289:1312	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	1	70	theme	biomarker	224:232	arg1	patients					243:250	biomarker negative patients	224:250	biomarker negative patients undergoing percutaneous coronary intervention (PCI)	224:302	OBJECTIVES The safety and efficacy of an initial intravenous bolus of low-dose heparin (40 IU/kg) was evaluated in biomarker negative patients undergoing percutaneous coronary intervention (PCI).					
32703583	9	71	theme	death	1081:1085	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	71	theme	death	1081:1085	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	71	theme	death	1081:1085	arg1	death					1081:1085	cardiac death	1073:1085	cardiac death	1073:1085	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	71	theme	death	1081:1085	arg1	bleeding					1184:1191	bleeding	1184:1191	bleeding	1184:1191	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	71	theme	death	1081:1085	arg1	point					1046:1050	The primary end point	1030:1050	The primary end point	1030:1050	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	71	theme	death	1081:1085	arg1	composite					1060:1068	the composite	1056:1068	the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days	1056:1206	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	0	72	theme	Coronary	13:20	arg1	Intervention					22:33	Percutaneous Coronary Intervention	0:33	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients	0:106	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	4	73	theme	bleeding	540:547	arg1	risk					532:535	the risk	528:535	the risk of bleeding	528:547	The higher dose of 100 IU/kg may increase the risk of bleeding.					
32703583	9	74	theme	myocardial	1088:1097	arg1	infarction					1099:1108	myocardial infarction	1088:1108	myocardial infarction (MI)	1088:1113	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	74	theme	myocardial	1088:1097	arg1	MI					1111:1112	MI	1111:1112	MI	1111:1112	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	3	75	theme	heparin	432:438	arg1	dose					424:427	the ideal dose	414:427	the ideal dose of heparin	414:438	However, the ideal dose of heparin has not been evaluated in a randomized trial.					
32703583	5	76	theme	low-dose	570:577	arg1	heparin					579:585	low-dose heparin	570:585	low-dose heparin	570:585	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	11	77	from	TVR	1406:1408	arg1	%					1384:1384	1.0%	1381:1384	1.0%	1381:1384	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	77	from	TVR	1406:1408	arg1	%					1416:1416	0.7%	1413:1416	0.7%	1413:1416	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	77	from	TVR	1406:1408	arg1	%					1396:1396	3.1%	1393:1396	3.1%	1393:1396	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	5	78	dep	times	653:657	arg1	ACT					660:662	ACT	660:662	ACT	660:662	An initial bolus of low-dose heparin may be advantageous to avoid supratherapeutic activating clotting times (ACT) while still allowing for the administration of additional heparin if the ACT is subtherapeutic.					
32703583	15	79	theme	initial	1626:1632	arg1	strategy					1634:1641	An initial strategy	1623:1641	An initial strategy of low-dose heparin	1623:1661	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	15	80	from	complications	1708:1720	arg1	patients					1744:1751	biomarker negative patients	1725:1751	biomarker negative patients who undergo transfemoral PCI	1725:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	15	81	theme	heparin	1655:1661	arg1	strategy					1634:1641	An initial strategy	1623:1641	An initial strategy of low-dose heparin	1623:1661	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	0	82	theme	Initial	43:49	arg1	Bolus					51:55	an Initial Bolus	40:55	an Initial Bolus of Low-Dose Heparin	40:75	Percutaneous Coronary Intervention With an Initial Bolus of Low-Dose Heparin in Biomarker-Negative Patients.					
32703583	15	83	theme	bleeding	1699:1706	arg1	complications					1708:1720	low ischemic and bleeding complications	1682:1720	low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI	1682:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
32703583	11	84	from	MI	1387:1388	arg1	%					1384:1384	1.0%	1381:1384	1.0%	1381:1384	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	84	from	MI	1387:1388	arg1	%					1416:1416	0.7%	1413:1416	0.7%	1413:1416	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	11	84	from	MI	1387:1388	arg1	%					1396:1396	3.1%	1393:1396	3.1%	1393:1396	The clinical event rates were low: the primary end point occurred in 5.3%, cardiac death in 1.0%, MI in 3.1%, urgent TVR in 0.7% and major bleeding in 1.9%.					
32703583	6	85	theme	transfemoral	838:849	arg1	PCI					851:853	elective transfemoral PCI	829:853	elective transfemoral PCI	829:853	METHODS From January 2008 to February 2020, 904 patients undergoing elective transfemoral PCI received an initial bolus of 40 IU/kg of heparin.					
32703583	13	86	theme	profound	1506:1513	arg1	thrombocytopenia					1515:1530	profound thrombocytopenia	1506:1530	profound thrombocytopenia	1506:1530	No patients developed profound thrombocytopenia.					
32703583	9	87	theme	urgent	1116:1121	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	87	theme	urgent	1116:1121	arg1	TVR					1156:1158	TVR	1156:1158	TVR	1156:1158	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	2	88	theme	heparin	343:349	arg1	70-100 IU/kg					327:338	70-100 IU/kg	327:338	70-100 IU/kg of heparin	327:349	BACKGROUND A bolus of 70-100 IU/kg of heparin is currently recommended for patients undergoing PCI.					
32703583	7	89	theme	transradial	928:938	arg1	PCI					940:942	transradial PCI	928:942	transradial PCI	928:942	Patients who underwent transradial PCI were not included.					
32703583	9	90	theme	vessel	1130:1135	arg1	revascularization					1137:1153	urgent target vessel revascularization	1116:1153	urgent target vessel revascularization (TVR) for ischemia	1116:1172	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	9	90	theme	vessel	1130:1135	arg1	TVR					1156:1158	TVR	1156:1158	TVR	1156:1158	The primary end point was the composite of cardiac death, myocardial infarction (MI), urgent target vessel revascularization (TVR) for ischemia, or major bleeding within 30 days after PCI.					
32703583	2	91	theme	70-100 IU/kg	327:338	arg1	bolus					318:322	A bolus	316:322	A bolus of 70-100 IU/kg of heparin	316:349	BACKGROUND A bolus of 70-100 IU/kg of heparin is currently recommended for patients undergoing PCI.					
32703583	10	92	theme	clotting	1255:1262	arg1	time					1264:1267	The initial mean activating clotting time	1227:1267	The initial mean activating clotting time	1227:1267	RESULTS The initial mean activating clotting time was 235.4 ± 26.6 s.					
32703583	15	93	theme	transfemoral	1765:1776	arg1	PCI					1778:1780	transfemoral PCI	1765:1780	transfemoral PCI	1765:1780	CONCLUSION An initial strategy of low-dose heparin is associated with low ischemic and bleeding complications in biomarker negative patients who undergo transfemoral PCI.					
33996624	1	0	dep	cancer	334:339	arg1	prevention					347:356	prevention	347:356	prevention	347:356	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	5	1	theme	cytokine	871:878	arg1	changes					880:886	inflammatory cytokine changes	858:886	inflammatory cytokine changes in CAC mice	858:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	1	2	theme	flora	288:292	arg1	structure					260:268	the structure	256:268	the structure of the intestinal flora	256:292	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	0	3	theme	Colorectal	107:116	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	2	4	theme	B	379:379	arg1	23-acetate					381:390	Alisol B 23-acetate	372:390	Alisol B 23-acetate (AB23A)	372:398	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	2	4	theme	B	379:379	arg1	triterpenoid					425:436	triterpenoid	425:436	triterpenoid	425:436	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	2	4	theme	B	379:379	arg1	AB23A					393:397	AB23A	393:397	AB23A	393:397	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	8	5	theme	increased	1333:1341	arg1	growth					1343:1348	the increased growth	1329:1348	the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella	1329:1417	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	6	6	theme	TLR	972:974	arg1	activation					958:967	the activation	954:967	the activation of TLR, NF-κB and MAPK	954:990	AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK.					
33996624	7	7	theme	junction	1117:1124	arg1	proteins					1126:1133	tight junction proteins	1111:1133	tight junction proteins	1111:1133	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	4	8	theme	potential	626:634	arg1	prevention					636:645	the potential prevention	622:645	the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	622:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	5	9	from	changes	880:886	arg1	mice					895:898	CAC mice	891:898	CAC mice	891:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	3	10	theme	gastrointestinal	555:570	arg1	diseases					572:579	gastrointestinal diseases	555:579	gastrointestinal diseases in China	555:588	Alismatis rhizoma is often used clinically to treat gastrointestinal diseases in China.					
33996624	3	11	from	diseases	572:579	arg1	China					584:588	China	584:588	China	584:588	Alismatis rhizoma is often used clinically to treat gastrointestinal diseases in China.					
33996624	1	12	theme	new	299:301	arg1	hotspot					303:309	a new hotspot	297:309	a new hotspot for colitis-associated cancer (CAC) prevention or treatment	297:369	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	1	12	theme	new	299:301	arg1	Hunting					208:214	Hunting	208:214	Hunting	208:214	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	3	13	theme	Alismatis	503:511	arg1	rhizoma					513:519	Alismatis rhizoma	503:519	Alismatis rhizoma	503:519	Alismatis rhizoma is often used clinically to treat gastrointestinal diseases in China.					
33996624	7	14	theme	proteins	1126:1133	arg1	mucin-2					1081:1087	mucin-2	1081:1087	mucin-2	1081:1087	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	7	14	theme	proteins	1126:1133	arg1	expression					1097:1106	the expression	1093:1106	the expression of tight junction proteins	1093:1133	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	8	15	theme	pathogenic	1254:1263	arg1	bacteria					1265:1272	pathogenic bacteria	1254:1272	pathogenic bacteria	1254:1272	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	4	16	theme	azoxymethane	680:691	arg1	models					670:675	male mouse models	659:675	male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	659:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	5	17	theme	body	779:782	arg1	loss					791:794	the body weight loss	775:794	the body weight loss	775:794	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	2	18	theme	dietary	489:495	arg1	herb					497:500	dietary herb	489:500	dietary herb	489:500	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	8	19	theme	bacteria	1265:1272	arg1	diversity					1218:1226	high microbial diversity	1203:1226	high microbial diversity	1203:1226	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	8	19	theme	bacteria	1265:1272	arg1	expansion					1241:1249	the reduced expansion	1229:1249	the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia	1229:1322	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	8	19	theme	bacteria	1265:1272	arg1	growth					1343:1348	the increased growth	1329:1348	the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella	1329:1417	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	5	20	theme	disease	797:803	arg1	index					814:818	disease activity index	797:818	disease activity index	797:818	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	4	21	theme	mouse	664:668	arg1	models					670:675	male mouse models	659:675	male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	659:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	0	22	theme	B	7:7	arg1	23-Acetate					9:18	Alisol B 23-Acetate	0:18	Alisol B 23-Acetate	0:18	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	7	23	theme	ERK	1051:1053	arg1	phosphorylation					1027:1041	the phosphorylation	1023:1041	the phosphorylation of p38, ERK, and JNK	1023:1062	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	4	24	from	prevention	636:645	arg1	models					670:675	male mouse models	659:675	male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	659:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	5	25	from	index	814:818	arg1	mice					895:898	CAC mice	891:898	CAC mice	891:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	1	26	theme	natural	220:226	arg1	compounds					228:236	natural compounds	220:236	natural compounds that can modulate the structure of the intestinal flora	220:292	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	0	27	theme	Alisol	0:5	arg1	23-Acetate					9:18	Alisol B 23-Acetate	0:18	Alisol B 23-Acetate	0:18	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	7	28	theme	tight	1111:1115	arg1	proteins					1126:1133	tight junction proteins	1111:1133	tight junction proteins	1111:1133	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	8	29	theme	microbial	1208:1216	arg1	diversity					1218:1226	high microbial diversity	1203:1226	high microbial diversity	1203:1226	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	9	30	contain	has	1449:1451	arg1	AB23A					1443:1447	AB23A	1443:1447	AB23A	1443:1447	These data reveal that AB23A has the potential to be used to treat CAC in the future.					
33996624	9	30	contain	has	1449:1451	arg2	potential					1457:1465	the potential to be used to treat CAC in the future	1453:1503	the potential to be used to treat CAC in the future	1453:1503	These data reveal that AB23A has the potential to be used to treat CAC in the future.					
33996624	8	31	theme	mice	1175:1178	arg1	microbiota					1144:1153	The gut microbiota	1136:1153	The gut microbiota of AB23A-interfered mice	1136:1178	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	5	32	theme	activity	805:812	arg1	index					814:818	disease activity index	797:818	disease activity index	797:818	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	0	33	theme	Gut	159:161	arg1	Microbiota					163:172	Gut Microbiota	159:172	Gut Microbiota	159:172	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	8	34	theme	AB23A-interfered	1158:1173	arg1	mice					1175:1178	AB23A-interfered mice	1158:1178	AB23A-interfered mice	1158:1178	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	4	35	theme	CAC	740:742	arg1	models					670:675	male mouse models	659:675	male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	659:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	4	36	theme	AB23A	650:654	arg1	prevention					636:645	the potential prevention	622:645	the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	622:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	2	37	theme	Alismatis	447:455	arg1	rhizoma					457:463	Alismatis rhizoma	447:463	Alismatis rhizoma which is well known for dietary herb	447:500	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	1	38	theme	colitis-associated	315:332	arg1	CAC					342:344	CAC	342:344	CAC	342:344	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	1	38	theme	colitis-associated	315:332	arg1	cancer					334:339	colitis-associated cancer	315:339	colitis-associated cancer (CAC)	315:345	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33996624	2	39	located	found	438:442	arg2	triterpenoid					425:436	triterpenoid	425:436	triterpenoid	425:436	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	2	39	located	found	438:442	arg1	rhizoma					457:463	Alismatis rhizoma	447:463	Alismatis rhizoma which is well known for dietary herb	447:500	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	2	39	located	found	438:442	arg2	23-acetate					381:390	Alisol B 23-acetate	372:390	Alisol B 23-acetate (AB23A)	372:398	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	4	40	theme	-induced	731:738	arg1	CAC					740:742	dextran sulfate sodium (DSS)-induced CAC	703:742	dextran sulfate sodium (DSS)-induced CAC	703:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	5	41	theme	colon	821:825	arg1	load					833:836	colon tumor load	821:836	colon tumor load	821:836	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	0	42	theme	Sodium	53:58	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	6	43	theme	remarkable	929:938	arg1	reductions					940:949	remarkable reductions	929:949	remarkable reductions in the activation of TLR, NF-κB and MAPK	929:990	AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK.					
33996624	5	44	theme	tumor	827:831	arg1	load					833:836	colon tumor load	821:836	colon tumor load	821:836	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	8	45	theme	bacteria	1353:1360	arg1	diversity					1218:1226	high microbial diversity	1203:1226	high microbial diversity	1203:1226	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	8	45	theme	bacteria	1353:1360	arg1	expansion					1241:1249	the reduced expansion	1229:1249	the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia	1229:1322	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	8	45	theme	bacteria	1353:1360	arg1	growth					1343:1348	the increased growth	1329:1348	the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella	1329:1417	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	4	46	theme	male	659:662	arg1	models					670:675	male mouse models	659:675	male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC	659:742	In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC.					
33996624	0	47	theme	Azoxymethane/Dextran	32:51	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	0	48	theme	Microbiota	163:172	arg1	Composition					144:154	the Composition	140:154	the Composition of Gut Microbiota	140:172	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	6	49	theme	NF-κB	977:981	arg1	activation					958:967	the activation	954:967	the activation of TLR, NF-κB and MAPK	954:990	AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK.					
33996624	0	50	theme	Male	76:79	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	7	51	theme	p38	1046:1048	arg1	phosphorylation					1027:1041	the phosphorylation	1023:1041	the phosphorylation of p38, ERK, and JNK	1023:1062	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	0	52	theme	Intestinal	188:197	arg1	Barrier					199:205	Intestinal Barrier	188:205	Intestinal Barrier	188:205	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	5	53	theme	AB23A	745:749	arg1	intervention					751:762	AB23A intervention	745:762	AB23A intervention	745:762	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	5	54	from	load	833:836	arg1	mice					895:898	CAC mice	891:898	CAC mice	891:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	2	55	theme	Alisol	372:377	arg1	23-acetate					381:390	Alisol B 23-acetate	372:390	Alisol B 23-acetate (AB23A)	372:398	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	2	55	theme	Alisol	372:377	arg1	triterpenoid					425:436	triterpenoid	425:436	triterpenoid	425:436	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	2	55	theme	Alisol	372:377	arg1	AB23A					393:397	AB23A	393:397	AB23A	393:397	Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb.					
33996624	0	56	theme	Sulfate-Induced	60:74	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	6	57	from	reductions	940:949	arg1	activation					958:967	the activation	954:967	the activation of TLR, NF-κB and MAPK	954:990	AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK.					
33996624	7	58	theme	JNK	1060:1062	arg1	phosphorylation					1027:1041	the phosphorylation	1023:1041	the phosphorylation of p38, ERK, and JNK	1023:1062	AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins.					
33996624	5	59	from	loss	791:794	arg1	mice					895:898	CAC mice	891:898	CAC mice	891:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	5	60	theme	CAC	891:893	arg1	mice					895:898	CAC mice	891:898	CAC mice	891:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	6	61	theme	AB23A	901:905	arg1	intervention					907:918	AB23A intervention	901:918	AB23A intervention	901:918	AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK.					
33996624	5	62	theme	weight	784:789	arg1	loss					791:794	the body weight loss	775:794	the body weight loss	775:794	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	8	63	theme	such	1275:1278	arg1	bacteria					1265:1272	pathogenic bacteria	1254:1272	pathogenic bacteria	1254:1272	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	8	64	theme	gut	1140:1142	arg1	microbiota					1144:1153	The gut microbiota	1136:1153	The gut microbiota of AB23A-interfered mice	1136:1178	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	5	65	from	injury	846:851	arg1	mice					895:898	CAC mice	891:898	CAC mice	891:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	6	66	theme	MAPK	987:990	arg1	activation					958:967	the activation	954:967	the activation of TLR, NF-κB and MAPK	954:990	AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK.					
33996624	0	67	theme	Colitis-Associated	88:105	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	8	68	theme	reduced	1233:1239	arg1	expansion					1241:1249	the reduced expansion	1229:1249	the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia	1229:1322	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	5	69	theme	tissue	839:844	arg1	injury					846:851	tissue injury	839:851	tissue injury	839:851	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	0	70	theme	Murine	81:86	arg1	Cancer					118:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer	32:123	Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier.					
33996624	8	71	theme	high	1203:1206	arg1	diversity					1218:1226	high microbial diversity	1203:1226	high microbial diversity	1203:1226	The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella.					
33996624	3	72	used	used	530:533	arg2	rhizoma					513:519	Alismatis rhizoma	503:519	Alismatis rhizoma	503:519	Alismatis rhizoma is often used clinically to treat gastrointestinal diseases in China.					
33996624	5	73	theme	inflammatory	858:869	arg1	changes					880:886	inflammatory cytokine changes	858:886	inflammatory cytokine changes in CAC mice	858:898	AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice.					
33996624	1	74	theme	intestinal	277:286	arg1	flora					288:292	the intestinal flora	273:292	the intestinal flora	273:292	Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment.					
33723263	5	0	theme	liposome	786:793	arg1	composition					795:805	liposome composition	786:805	liposome composition	786:805	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	5	1	theme	Pickering-like	647:660	arg1	system					671:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	7	2	theme	motility	1031:1038	arg1	abilities					1057:1065	The biocompatibility, motility and partitioning abilities	1009:1065	The biocompatibility, motility and partitioning abilities of this droplet system	1009:1088	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	7	3	theme	new	1097:1099	arg1	directions					1101:1110	new directions	1097:1110	new directions to pursue research in motion-related biological processes	1097:1168	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	4	4	theme	biocompatibility	524:539	arg1	issues					541:546	biocompatibility issues	524:546	biocompatibility issues caused by the presence of oil phases	524:583	However, most of these systems are unsuitable for biological applications due to biocompatibility issues caused by the presence of oil phases.					
33723263	5	5	theme	emulsion	662:669	arg1	system					671:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	3	6	theme	effect	382:387	arg1	use					361:363	use	361:363	use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems	361:440	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	6	7	theme	PEG/dextran polymer	931:949	arg1	gradient					951:958	a PEG/dextran polymer gradient	929:958	a PEG/dextran polymer gradient	929:958	We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient.					
33723263	5	8	theme	biocompatible	603:615	arg1	system					671:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	2	9	theme	living	316:321	arg1	systems					323:329	living systems	316:329	living systems	316:329	One behaviour that is receiving particular attention is motility, due to its biotechnological potential and ubiquity in living systems.					
33723263	7	10	from	research	1122:1129	arg1	processes					1160:1168	motion-related biological processes	1134:1168	motion-related biological processes	1134:1168	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	3	11	from	motion	400:405	arg1	systems					434:440	water/oil (w/o) droplet systems	410:440	water/oil (w/o) droplet systems	410:440	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	3	12	theme	Marangoni	372:380	arg1	effect					382:387	the Marangoni effect	368:387	the Marangoni effect	368:387	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	4	13	theme	biological	493:502	arg1	applications					504:515	biological applications	493:515	biological applications due to biocompatibility issues caused by the presence of oil phases	493:583	However, most of these systems are unsuitable for biological applications due to biocompatibility issues caused by the presence of oil phases.					
33723263	6	14	theme	directional	968:978	arg1	motion					980:985	directional motion	968:985	directional motion down to the gradient	968:1006	We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient.					
33723263	0	15	theme	motile	12:17	arg1	droplets					43:50	motile aqueous phase-separated droplets	12:50	motile aqueous phase-separated droplets	12:50	Engineering motile aqueous phase-separated droplets via liposome stabilisation.					
33723263	7	16	theme	motion-related	1134:1147	arg1	processes					1160:1168	motion-related biological processes	1134:1168	motion-related biological processes	1134:1168	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	5	17	theme	liposome-stabilised	692:710	arg1	droplets					723:730	liposome-stabilised cell-sized droplets	692:730	liposome-stabilised cell-sized droplets	692:730	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	3	18	theme	water/oil	410:418	arg1	systems					434:440	water/oil (w/o) droplet systems	410:440	water/oil (w/o) droplet systems	410:440	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	5	19	theme	PEG/dextran	635:645	arg1	system					671:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system	601:676	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	0	20	theme	liposome	56:63	arg1	stabilisation					65:77	liposome stabilisation	56:77	liposome stabilisation	56:77	Engineering motile aqueous phase-separated droplets via liposome stabilisation.					
33723263	0	21	theme	phase-separated	27:41	arg1	droplets					43:50	motile aqueous phase-separated droplets	12:50	motile aqueous phase-separated droplets	12:50	Engineering motile aqueous phase-separated droplets via liposome stabilisation.					
33723263	0	22	theme	aqueous	19:25	arg1	droplets					43:50	motile aqueous phase-separated droplets	12:50	motile aqueous phase-separated droplets	12:50	Engineering motile aqueous phase-separated droplets via liposome stabilisation.					
33723263	4	23	theme	phases	578:583	arg1	presence					562:569	the presence	558:569	the presence of oil phases	558:583	However, most of these systems are unsuitable for biological applications due to biocompatibility issues caused by the presence of oil phases.					
33723263	7	24	theme	system	1083:1088	arg1	abilities					1057:1065	The biocompatibility, motility and partitioning abilities	1009:1065	The biocompatibility, motility and partitioning abilities of this droplet system	1009:1088	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	1	25	theme	functional	121:130	arg1	compartments					132:143	functional compartments	121:143	functional compartments that mimic cellular behaviours from the bottom-up	121:193	There are increasing efforts to engineer functional compartments that mimic cellular behaviours from the bottom-up.					
33723263	2	26	from	potential	290:298	arg1	systems					323:329	living systems	316:329	living systems	316:329	One behaviour that is receiving particular attention is motility, due to its biotechnological potential and ubiquity in living systems.					
33723263	4	27	theme	oil	574:576	arg1	phases					578:583	oil phases	574:583	oil phases	574:583	However, most of these systems are unsuitable for biological applications due to biocompatibility issues caused by the presence of oil phases.					
33723263	3	28	theme	droplet	426:432	arg1	systems					434:440	water/oil (w/o) droplet systems	410:440	water/oil (w/o) droplet systems	410:440	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	3	29	theme	Many	332:335	arg1	platforms					346:354	Many existing platforms	332:354	Many existing platforms	332:354	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	6	30	theme	chemotaxis	887:896	arg1	capable					867:873	capable	867:873	capable	867:873	We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient.					
33723263	6	30	theme	chemotaxis	887:896	arg1	compartments					850:861	the compartments	846:861	the compartments	846:861	We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient.					
33723263	7	31	theme	droplet	1075:1081	arg1	system					1083:1088	this droplet system	1070:1088	this droplet system	1070:1088	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	3	32	theme	w/o	421:423	arg1	systems					434:440	water/oil (w/o) droplet systems	410:440	water/oil (w/o) droplet systems	410:440	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	6	33	theme	down	987:990	arg1	motion					980:985	directional motion	968:985	directional motion down to the gradient	968:1006	We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient.					
33723263	3	34	theme	existing	337:344	arg1	platforms					346:354	Many existing platforms	332:354	Many existing platforms	332:354	Many existing platforms make use of the Marangoni effect to achieve motion in water/oil (w/o) droplet systems.					
33723263	6	35	theme	negative	878:885	arg1	chemotaxis					887:896	negative chemotaxis	878:896	negative chemotaxis	878:896	We demonstrate that the compartments are capable of negative chemotaxis: these droplets can respond to a PEG/dextran polymer gradient through directional motion down to the gradient.					
33723263	2	36	from	ubiquity	304:311	arg1	systems					323:329	living systems	316:329	living systems	316:329	One behaviour that is receiving particular attention is motility, due to its biotechnological potential and ubiquity in living systems.					
33723263	5	37	theme	cell-sized	712:721	arg1	droplets					723:730	liposome-stabilised cell-sized droplets	692:730	liposome-stabilised cell-sized droplets	692:730	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	2	38	theme	particular	228:237	arg1	attention					239:247	particular attention	228:247	particular attention	228:247	One behaviour that is receiving particular attention is motility, due to its biotechnological potential and ubiquity in living systems.					
33723263	7	39	theme	biocompatibility	1013:1028	arg1	abilities					1057:1065	The biocompatibility, motility and partitioning abilities	1009:1065	The biocompatibility, motility and partitioning abilities of this droplet system	1009:1088	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	2	40	theme	biotechnological	273:288	arg1	potential					290:298	its biotechnological potential	269:298	its biotechnological potential	269:298	One behaviour that is receiving particular attention is motility, due to its biotechnological potential and ubiquity in living systems.					
33723263	1	41	theme	cellular	156:163	arg1	behaviours					165:174	cellular behaviours	156:174	cellular behaviours	156:174	There are increasing efforts to engineer functional compartments that mimic cellular behaviours from the bottom-up.					
33723263	7	42	theme	biological	1149:1158	arg1	processes					1160:1168	motion-related biological processes	1134:1168	motion-related biological processes	1134:1168	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	7	43	theme	partitioning	1044:1055	arg1	abilities					1057:1065	The biocompatibility, motility and partitioning abilities	1009:1065	The biocompatibility, motility and partitioning abilities of this droplet system	1009:1088	The biocompatibility, motility and partitioning abilities of this droplet system offers new directions to pursue research in motion-related biological processes.					
33723263	5	44	dep	biocompatible	603:615	arg1	w/w					630:632	w/w	630:632	w/w	630:632	Here we report a biocompatible all aqueous (w/w) PEG/dextran Pickering-like emulsion system consisting of liposome-stabilised cell-sized droplets, where the stability can be easily tuned by adjusting liposome composition and concentration.					
33723263	4	45	theme	due	517:519	arg1	applications					504:515	biological applications	493:515	biological applications due to biocompatibility issues caused by the presence of oil phases	493:583	However, most of these systems are unsuitable for biological applications due to biocompatibility issues caused by the presence of oil phases.					
32122507	0	0	theme	crosslinker	83:93	arg1	Effects					38:44	Effects	38:44	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.	0:94	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	1	1	theme	low	173:175	arg1	solution					202:209	a low concentrated acetic acid solution	171:209	a low concentrated acetic acid solution	171:209	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	1	2	theme	co-spinning	250:260	arg1	agent					262:266	co-spinning agent	250:266	co-spinning agent	250:266	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	5	3	link	crosslinked	886:896	arg1	mats					898:901	the ionically crosslinked mats	872:901	the ionically crosslinked mats	872:901	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	5	4	theme	promising	934:942	arg1	products					955:962	extremely promising biomedical products	924:962	extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm	924:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	1	5	theme	concentrated	177:188	arg1	acid					197:200	concentrated acetic acid	177:200	a low concentrated acetic acid solution	171:209	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	5	6	theme	thin	1021:1024	arg1	nanofibers					1041:1050	thin and homogenous nanofibers	1021:1050	thin and homogenous nanofibers with a diameter of 200 nm	1021:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	2	7	theme	solution	341:348	arg1	viscosity					350:358	the solution viscosity	337:358	the solution viscosity was found to correspond to a better-defined morphology	337:413	Different solutions were rheologically characterized and increasing the solution viscosity was found to correspond to a better-defined morphology.					
32122507	3	8	dep	stabilize	509:517	arg1	to					506:507	to	506:507	to	506:507	The membranes were first subjected to a coagulation process with different baths in order to stabilize chitosan and the mats were found not able to withstand a strongly basic environment.					
32122507	5	9	theme	homogenous	1030:1039	arg1	nanofibers					1041:1050	thin and homogenous nanofibers	1021:1050	thin and homogenous nanofibers with a diameter of 200 nm	1021:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	1	10	theme	acetic	190:195	arg1	acid					197:200	concentrated acetic acid	177:200	a low concentrated acetic acid solution	171:209	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	1	11	theme	acid	197:200	arg1	solution					202:209	a low concentrated acetic acid solution	171:209	a low concentrated acetic acid solution	171:209	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	0	12	theme	electrospun	15:25	arg1	membranes					27:35	Chitosan-based electrospun membranes	0:35	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.	0:94	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	3	13	theme	basic	585:589	arg1	environment					591:601	a strongly basic environment	574:601	a strongly basic environment	574:601	The membranes were first subjected to a coagulation process with different baths in order to stabilize chitosan and the mats were found not able to withstand a strongly basic environment.					
32122507	4	14	theme	stable	702:707	arg1	mats					709:712	stable mats	702:712	stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques	702:794	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
32122507	0	15	theme	Chitosan-based	0:13	arg1	membranes					27:35	Chitosan-based electrospun membranes	0:35	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.	0:94	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	1	16	theme	Chitosan-based	96:109	arg1	membranes					111:119	Chitosan-based membranes	96:119	Chitosan-based membranes	96:119	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	3	17	theme	coagulation	456:466	arg1	process					468:474	a coagulation process	454:474	a coagulation process	454:474	The membranes were first subjected to a coagulation process with different baths in order to stabilize chitosan and the mats were found not able to withstand a strongly basic environment.					
32122507	4	18	dep	thermogravimetric	749:765	arg1	techniques					785:794	techniques	785:794	techniques	785:794	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
32122507	5	19	dep	products	955:962	arg1	characterized					1004:1016	characterized	1004:1016	characterized by thin and homogenous nanofibers with a diameter of 200 nm	1004:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	5	19	dep	products	955:962	arg1	biocompatible					986:998	biocompatible	986:998	biocompatible	986:998	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	5	20	theme	biomedical	944:953	arg1	products					955:962	extremely promising biomedical products	924:962	extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm	924:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	4	21	theme	crosslinking	644:655	arg1	approach					657:664	a physical and a chemical crosslinking approach	618:664	a physical and a chemical crosslinking approach	618:664	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
32122507	5	22	theme	cell	1122:1125	arg1	viability					1127:1135	cell viability	1122:1135	cell viability	1122:1135	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	5	23	theme	200 nm	1071:1076	arg1	diameter					1059:1066	a diameter	1057:1066	a diameter of 200 nm	1057:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	3	24	theme	different	481:489	arg1	baths					491:495	different baths	481:495	different baths	481:495	The membranes were first subjected to a coagulation process with different baths in order to stabilize chitosan and the mats were found not able to withstand a strongly basic environment.					
32122507	5	25	with	nanofibers	1041:1050	arg1	diameter					1059:1066	a diameter	1057:1066	a diameter of 200 nm	1057:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	4	26	theme	agent	854:858	arg1	elimination					823:833	the elimination	819:833	the elimination of the co-spinning agent	819:858	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
32122507	5	27	theme	crosslinked	886:896	arg1	mats					898:901	the ionically crosslinked mats	872:901	the ionically crosslinked mats	872:901	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	0	28	theme	viscosity	58:66	arg1	Effects					38:44	Effects	38:44	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.	0:94	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	5	29	theme	products	955:962	arg1	class					915:919	a class	913:919	a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm	913:1076	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	0	30	theme	solution	49:56	arg1	viscosity					58:66	solution viscosity	49:66	solution viscosity	49:66	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	5	31	theme	ideal	1096:1100	arg1	structure					1102:1110	the ideal structure	1092:1110	the ideal structure to foster cell viability	1092:1135	Above all, the ionically crosslinked mats represent a class of extremely promising biomedical products being probably highly biocompatible and characterized by thin and homogenous nanofibers with a diameter of 200 nm, thus showing the ideal structure to foster cell viability.					
32122507	2	32	theme	Different	269:277	arg1	solutions					279:287	Different solutions	269:287	Different solutions	269:287	Different solutions were rheologically characterized and increasing the solution viscosity was found to correspond to a better-defined morphology.					
32122507	1	33	theme	ethylene	231:238	arg1	poly					226:229	poly	226:229	poly(ethylene oxide)	226:245	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	1	33	theme	ethylene	231:238	arg1	oxide					240:244	ethylene oxide	231:244	ethylene oxide	231:244	Chitosan-based membranes were prepared via electrospinning technique using a low concentrated acetic acid solution as solvent and poly(ethylene oxide) as co-spinning agent.					
32122507	0	34	dep	membranes	27:35	arg1	Effects					38:44	Effects	38:44	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.	0:94	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	4	35	theme	physical	620:627	arg1	approach					657:664	a physical and a chemical crosslinking approach	618:664	a physical and a chemical crosslinking approach	618:664	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
32122507	0	36	theme	coagulant	69:77	arg1	Effects					38:44	Effects	38:44	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.	0:94	Chitosan-based electrospun membranes: Effects of solution viscosity, coagulant and crosslinker.					
32122507	4	37	theme	co-spinning	842:852	arg1	agent					854:858	the co-spinning agent	838:858	the co-spinning agent	838:858	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
32122507	2	38	theme	better-defined	389:402	arg1	morphology					404:413	a better-defined morphology	387:413	a better-defined morphology	387:413	Different solutions were rheologically characterized and increasing the solution viscosity was found to correspond to a better-defined morphology.					
32122507	4	39	theme	chemical	635:642	arg1	approach					657:664	a physical and a chemical crosslinking approach	618:664	a physical and a chemical crosslinking approach	618:664	Subsequently, a physical and a chemical crosslinking approach were separately optimized to obtain stable mats whose composition was assessed via thermogravimetric and spectroscopic techniques, proving in both cases the elimination of the co-spinning agent.					
34521966	0	0	from	pathway	31:37	arg1	genome					47:52	host genome	42:52	host genome	42:52	Glycosaminoglycan biosynthesis pathway in host genome is associated with Helicobacter pylori infection.					
34521966	6	1	theme	reported	890:897	arg1	rs10004195					903:912	one previously reported SNP rs10004195	875:912	one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02)	875:943	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	0	2	theme	pylori	86:91	arg1	infection					93:101	Helicobacter pylori infection	73:101	Helicobacter pylori infection	73:101	Glycosaminoglycan biosynthesis pathway in host genome is associated with Helicobacter pylori infection.					
34521966	4	3	theme	gene-based	495:504	arg1	analyses					548:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	7	4	theme	infection	1072:1080	arg1	onset					1037:1041	onset	1037:1041	onset	1037:1041	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	7	4	theme	infection	1072:1080	arg1	progression					1047:1057	progression	1047:1057	progression	1047:1057	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	1	5	theme	extra-gastric	168:180	arg1	diseases					182:189	many gastric and extra-gastric diseases	151:189	many gastric and extra-gastric diseases	151:189	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	8	6	theme	infected	1224:1231	arg1	cases					1233:1237	H. pylori infected cases	1214:1237	H. pylori infected cases	1214:1237	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	4	7	theme	genetic	576:582	arg1	basis					584:588	the genetic basis	572:588	the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals	572:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	5	8	theme	microbiota	721:730	arg1	composition					685:695	composition	685:695	composition	685:695	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	5	8	theme	microbiota	721:730	arg1	function					701:708	function	701:708	function	701:708	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	6	9	theme	SNP	899:901	arg1	rs10004195					903:912	one previously reported SNP rs10004195	875:912	one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02)	875:943	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	4	10	theme	host	593:596	arg1	susceptibility					598:611	host susceptibility	593:611	host susceptibility to H. pylori infection in 480 Chinese individuals	593:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	4	11	theme	single	456:461	arg1	polymorphism					474:485	single nucleotide polymorphism	456:485	single nucleotide polymorphism	456:485	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	8	12	theme	microbiome	1094:1103	arg1	study					1117:1121	the gut microbiome association study	1086:1121	the gut microbiome association study	1086:1121	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	8	13	theme	several	1162:1168	arg1	pathways					1170:1177	several pathways	1162:1177	several pathways	1162:1177	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	4	14	from	basis	584:588	arg1	individuals					651:661	480 Chinese individuals	639:661	480 Chinese individuals	639:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	9	15	theme	H.	1286:1287	arg1	infection					1296:1304	H. pylori infection	1286:1304	H. pylori infection	1286:1304	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	1	16	theme	diseases	182:189	arg1	pathogen					139:146	a causative pathogen	127:146	a causative pathogen of many gastric and extra-gastric diseases	127:189	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	1	16	theme	diseases	182:189	arg1	pylori					117:122	Helicobacter pylori	104:122	Helicobacter pylori	104:122	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	4	17	from	susceptibility	598:611	arg1	individuals					651:661	480 Chinese individuals	639:661	480 Chinese individuals	639:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	9	18	theme	pylori	1289:1294	arg1	infection					1296:1304	H. pylori infection	1286:1304	H. pylori infection	1286:1304	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	3	19	theme	genome-wide	259:269	arg1	association					271:281	no genome-wide association	256:281	no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori	256:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	6	20	theme	several	789:795	arg1	genes					797:801	several genes	789:801	several genes	789:801	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	3	21	theme	homogenous	392:401	arg1	strain					403:408	different and relatively homogenous strain	367:408	different and relatively homogenous strain of H. pylori	367:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	4	22	dep	-based	487:492	arg1	polymorphism					474:485	single nucleotide polymorphism	456:485	single nucleotide polymorphism	456:485	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	0	23	theme	biosynthesis	18:29	arg1	pathway					31:37	Glycosaminoglycan biosynthesis pathway	0:37	Glycosaminoglycan biosynthesis pathway in host genome	0:52	Glycosaminoglycan biosynthesis pathway in host genome is associated with Helicobacter pylori infection.					
34521966	4	24	theme	nucleotide	463:472	arg1	polymorphism					474:485	single nucleotide polymorphism	456:485	single nucleotide polymorphism	456:485	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	3	25	dep	H.	413:414	arg1	pylori					416:421	H. pylori	413:421	H. pylori	413:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	6	26	from	rs10004195	903:912	arg1	P = 0.02					935:942	P = 0.02	935:942	P = 0.02	935:942	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	6	26	from	rs10004195	903:912	arg1	region					927:932	TLR1 gene region	917:932	TLR1 gene region (P = 0.02)	917:943	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	4	27	from	individuals	651:661	arg1	basis					584:588	the genetic basis	572:588	the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals	572:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	9	28	theme	host	1416:1419	arg1	susceptibility					1421:1434	host susceptibility	1416:1434	host susceptibility to H. pylori infection	1416:1457	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	0	29	theme	Glycosaminoglycan	0:16	arg1	pathway					31:37	Glycosaminoglycan biosynthesis pathway	0:37	Glycosaminoglycan biosynthesis pathway in host genome	0:52	Glycosaminoglycan biosynthesis pathway in host genome is associated with Helicobacter pylori infection.					
34521966	8	30	theme	gut	1090:1092	arg1	study					1117:1121	the gut microbiome association study	1086:1121	the gut microbiome association study	1086:1121	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	6	31	theme	gene	922:925	arg1	P = 0.02					935:942	P = 0.02	935:942	P = 0.02	935:942	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	6	31	theme	gene	922:925	arg1	region					927:932	TLR1 gene region	917:932	TLR1 gene region (P = 0.02)	917:943	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	1	32	theme	Helicobacter	104:115	arg1	pathogen					139:146	a causative pathogen	127:146	a causative pathogen of many gastric and extra-gastric diseases	127:189	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	1	32	theme	Helicobacter	104:115	arg1	pylori					117:122	Helicobacter pylori	104:122	Helicobacter pylori	104:122	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	8	33	theme	differential	1183:1194	arg1	abundance					1196:1204	differential abundance	1183:1204	differential abundance between H. pylori infected cases and controls	1183:1250	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	9	34	theme	susceptibility	1421:1434	arg1	basis					1407:1411	the basis	1403:1411	the basis of host susceptibility to H. pylori infection	1403:1457	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	3	35	theme	different	367:375	arg1	strain					403:408	different and relatively homogenous strain	367:408	different and relatively homogenous strain of H. pylori	367:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	9	36	theme	Chinese	1309:1315	arg1	population					1317:1326	Chinese population	1309:1326	Chinese population	1309:1326	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	7	37	theme	H.	1062:1063	arg1	infection					1072:1080	H. pylori infection	1062:1080	H. pylori infection	1062:1080	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	9	38	from	GWAS	1277:1280	arg1	population					1317:1326	Chinese population	1309:1326	Chinese population	1309:1326	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	7	39	theme	pylori	1065:1070	arg1	infection					1072:1080	H. pylori infection	1062:1080	H. pylori infection	1062:1080	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	9	40	theme	H.	1439:1440	arg1	infection					1449:1457	H. pylori infection	1439:1457	H. pylori infection	1439:1457	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	3	41	theme	H.	413:414	arg1	strain					403:408	different and relatively homogenous strain	367:408	different and relatively homogenous strain of H. pylori	367:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	8	42	dep	H.	1214:1215	arg1	pylori					1217:1222	H. pylori	1214:1222	H. pylori infected cases	1214:1237	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	3	43	theme	association	271:281	arg1	GWAS					292:295	GWAS	292:295	GWAS	292:295	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	3	43	theme	association	271:281	arg1	studies					283:289	no genome-wide association studies	256:289	no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori	256:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	9	44	theme	pylori	1442:1447	arg1	infection					1449:1457	H. pylori infection	1439:1457	H. pylori infection	1439:1457	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	4	45	theme	Chinese	643:649	arg1	individuals					651:661	480 Chinese individuals	639:661	480 Chinese individuals	639:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	0	46	theme	host	42:45	arg1	genome					47:52	host genome	42:52	host genome	42:52	Glycosaminoglycan biosynthesis pathway in host genome is associated with Helicobacter pylori infection.					
34521966	4	47	theme	pathway-based	510:522	arg1	analyses					548:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	5	48	theme	gut	717:719	arg1	microbiota					721:730	the gut microbiota	713:730	the gut microbiota	713:730	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	2	49	theme	population	233:242	arg1	half					214:217	half	214:217	half of the global population	214:242	It has infected about half of the global population.					
34521966	5	50	theme	pylori	743:748	arg1	infection					750:758	H. pylori infection cases and controls	740:777	H. pylori infection cases and controls	740:777	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	8	51	theme	H.	1214:1215	arg1	cases					1233:1237	H. pylori infected cases	1214:1237	H. pylori infected cases	1214:1237	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	5	52	dep	composition	685:695	arg1	the					681:683	the	681:683	the	681:683	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	3	53	theme	H.	302:303	arg1	infection					312:320	H. pylori infection	302:320	H. pylori infection	302:320	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	5	54	dep	infection	750:758	arg1	cases					760:764	cases	760:764	cases	760:764	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	5	54	dep	infection	750:758	arg1	controls					770:777	controls	770:777	controls	770:777	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	2	55	theme	global	226:231	arg1	population					233:242	the global population	222:242	the global population	222:242	It has infected about half of the global population.					
34521966	7	56	theme	related	996:1002	arg1	pathway					1004:1010	glycosaminoglycan biosynthesis related pathway	965:1010	glycosaminoglycan biosynthesis related pathway	965:1010	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	9	57	theme	first	1271:1275	arg1	GWAS					1277:1280	the first GWAS	1267:1280	the first GWAS for H. pylori infection in Chinese population	1267:1326	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	9	57	theme	first	1271:1275	arg1	paper					1258:1262	This paper	1253:1262	This paper	1253:1262	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	4	58	theme	association	536:546	arg1	analyses					548:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	3	59	theme	pylori	305:310	arg1	infection					312:320	H. pylori infection	302:320	H. pylori infection	302:320	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	8	60	dep	identified	1127:1136	arg1	had					1179:1181	had	1179:1181	identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls	1127:1250	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	3	61	contain	carried	359:365	arg2	strain					403:408	different and relatively homogenous strain	367:408	different and relatively homogenous strain of H. pylori	367:421	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	3	61	contain	carried	359:365	arg1	population					343:352	Chinese population	335:352	Chinese population	335:352	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	1	62	theme	causative	129:137	arg1	pathogen					139:146	a causative pathogen	127:146	a causative pathogen of many gastric and extra-gastric diseases	127:189	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	1	62	theme	causative	129:137	arg1	pylori					117:122	Helicobacter pylori	104:122	Helicobacter pylori	104:122	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	9	63	theme	microbial	1361:1369	arg1	data					1371:1374	the genetic and microbial data	1345:1374	the genetic and microbial data	1345:1374	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	4	64	theme	genome-wide	524:534	arg1	analyses					548:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	6	65	theme	pylori	835:840	arg1	P < 0.05					853:860	P < 0.05	853:860	P < 0.05	853:860	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	6	65	theme	pylori	835:840	arg1	infection					842:850	H. pylori infection	832:850	H. pylori infection (P < 0.05)	832:861	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	9	66	theme	genetic	1349:1355	arg1	data					1371:1374	the genetic and microbial data	1345:1374	the genetic and microbial data	1345:1374	This paper is the first GWAS for H. pylori infection in Chinese population, and we combined the genetic and microbial data to comprehensively discuss the basis of host susceptibility to H. pylori infection.					
34521966	5	67	theme	H.	740:741	arg1	infection					750:758	H. pylori infection cases and controls	740:777	H. pylori infection cases and controls	740:777	We also profiled the composition and function of the gut microbiota between H. pylori infection cases and controls.					
34521966	8	68	theme	association	1105:1115	arg1	study					1117:1121	the gut microbiome association study	1086:1121	the gut microbiome association study	1086:1121	In the gut microbiome association study, we identified 2 species, 3 genera and several pathways had differential abundance between H. pylori infected cases and controls.					
34521966	6	69	theme	H.	832:833	arg1	P < 0.05					853:860	P < 0.05	853:860	P < 0.05	853:860	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	6	69	theme	H.	832:833	arg1	infection					842:850	H. pylori infection	832:850	H. pylori infection (P < 0.05)	832:861	We found several genes and pathways associated with H. pylori infection (P < 0.05), replicated one previously reported SNP rs10004195 in TLR1 gene region (P = 0.02).					
34521966	7	70	theme	glycosaminoglycan	965:981	arg1	biosynthesis					983:994	glycosaminoglycan biosynthesis	965:994	glycosaminoglycan biosynthesis related pathway	965:1010	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	4	71	theme	susceptibility	598:611	arg1	basis					584:588	the genetic basis	572:588	the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals	572:661	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	0	72	theme	Helicobacter	73:84	arg1	infection					93:101	Helicobacter pylori infection	73:101	Helicobacter pylori infection	73:101	Glycosaminoglycan biosynthesis pathway in host genome is associated with Helicobacter pylori infection.					
34521966	7	73	theme	biosynthesis	983:994	arg1	pathway					1004:1010	glycosaminoglycan biosynthesis related pathway	965:1010	glycosaminoglycan biosynthesis related pathway	965:1010	We also found that glycosaminoglycan biosynthesis related pathway was associated with both onset and progression of H. pylori infection.					
34521966	1	74	theme	many	151:154	arg1	diseases					182:189	many gastric and extra-gastric diseases	151:189	many gastric and extra-gastric diseases	151:189	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	4	75	theme	pylori	619:624	arg1	infection					626:634	H. pylori infection	616:634	H. pylori infection	616:634	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	3	76	theme	Chinese	335:341	arg1	population					343:352	Chinese population	335:352	Chinese population	335:352	There were no genome-wide association studies (GWAS) for H. pylori infection conducted in Chinese population, who carried different and relatively homogenous strain of H. pylori.					
34521966	4	77	theme	-based	487:492	arg1	analyses					548:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses	451:555	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34521966	1	78	theme	gastric	156:162	arg1	diseases					182:189	many gastric and extra-gastric diseases	151:189	many gastric and extra-gastric diseases	151:189	Helicobacter pylori is a causative pathogen of many gastric and extra-gastric diseases.					
34521966	4	79	theme	H.	616:617	arg1	infection					626:634	H. pylori infection	616:634	H. pylori infection	616:634	In this work, we performed SNP (single nucleotide polymorphism)-based, gene-based and pathway-based genome-wide association analyses to investigate the genetic basis of host susceptibility to H. pylori infection in 480 Chinese individuals.					
34835980	0	0	theme	Body	60:63	arg1	Composition					65:75	Infant Body Composition	53:75	Infant Body Composition during Exclusive Breastfeeding	53:106	Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding.					
34835980	4	1	dep	Infant	471:476	arg1	BC					491:492	BC	491:492	BC	491:492	Infant and maternal BC was assessed with bioimpedance spectroscopy.					
34835980	7	2	theme	body	831:834	arg1	mass					836:839	lean body mass	826:839	lean body mass	826:839	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34835980	0	3	theme	Infant	53:58	arg1	Composition					65:75	Infant Body Composition	53:75	Infant Body Composition during Exclusive Breastfeeding	53:106	Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding.					
34835980	5	4	theme	infant	598:603	arg1	weight					611:616	infant birth weight	598:616	infant birth weight	598:616	Statistical analysis used linear regression accounting for infant birth weight.					
34835980	2	5	theme	body	242:245	arg1	BC					260:261	BC	260:261	BC	260:261	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	5	theme	body	242:245	arg1	composition					247:257	infant body composition	235:257	infant body composition (BC)	235:262	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	5	6	theme	birth	605:609	arg1	weight					611:616	infant birth weight	598:616	infant birth weight	598:616	Statistical analysis used linear regression accounting for infant birth weight.					
34835980	9	7	theme	BC	1099:1100	arg1	development					1077:1087	development	1077:1087	development of infant BC	1077:1100	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	5	8	used	used	560:563	arg2	analysis					551:558	Statistical analysis	539:558	Statistical analysis	539:558	Statistical analysis used linear regression accounting for infant birth weight.					
34835980	4	9	theme	bioimpedance	512:523	arg1	spectroscopy					525:536	bioimpedance spectroscopy	512:536	bioimpedance spectroscopy	512:536	Infant and maternal BC was assessed with bioimpedance spectroscopy.					
34835980	8	10	theme	24-h	969:972	arg1	intake					979:984	24-h milk intake	969:984	24-h milk intake	969:984	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	5	11	theme	Statistical	539:549	arg1	analysis					551:558	Statistical analysis	539:558	Statistical analysis	539:558	Statistical analysis used linear regression accounting for infant birth weight.					
34835980	9	12	theme	lactose	1024:1030	arg1	intake					1007:1012	24-h intake	1002:1012	24-h intake of HM and lactose as well as maternal adiposity	1002:1060	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	9	12	theme	lactose	1024:1030	arg1	adiposity					1052:1060	maternal adiposity	1043:1060	24-h intake of HM and lactose as well as maternal adiposity	1002:1060	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	6	13	theme	lactose	647:653	arg1	CDI					640:642	CDI	640:642	CDI of lactose	640:653	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	6	13	theme	lactose	647:653	arg1	intake					629:634	24-h milk intake	619:634	24-h milk intake	619:634	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	8	14	theme	milk	974:977	arg1	intake					979:984	24-h milk intake	969:984	24-h milk intake	969:984	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	9	15	theme	24-h	1002:1005	arg1	intake					1007:1012	24-h intake	1002:1012	24-h intake of HM and lactose as well as maternal adiposity	1002:1060	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	0	16	theme	Exclusive	84:92	arg1	Breastfeeding					94:106	Exclusive Breastfeeding	84:106	Exclusive Breastfeeding	84:106	Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding.					
34835980	5	17	theme	linear	565:570	arg1	regression					572:581	linear regression	565:581	linear regression accounting for infant birth weight	565:616	Statistical analysis used linear regression accounting for infant birth weight.					
34835980	3	18	theme	months	452:457	arg1	postpartum					459:468	2 months postpartum	450:468	2 months postpartum	450:468	HM samples were collected at 2 months postpartum.					
34835980	7	19	theme	lower	788:792	arg1	anthropometry					801:813	lower infant anthropometry	788:813	lower infant anthropometry	788:813	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34835980	1	20	theme	infant	150:155	arg1	growth					157:162	infant growth	150:162	infant growth	150:162	Human milk (HM) components may influence infant growth and development.					
34835980	0	21	theme	Milk	6:9	arg1	Lactose					11:17	Human Milk Lactose	0:17	Human Milk Lactose	0:17	Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding.					
34835980	2	22	theme	months	402:407	arg1	postpartum					409:418	3 months postpartum	400:418	3 months postpartum	400:418	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	7	23	theme	infant	794:799	arg1	anthropometry					801:813	lower infant anthropometry	788:813	lower infant anthropometry	788:813	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34835980	0	24	theme	Human	0:4	arg1	Lactose					11:17	Human Milk Lactose	0:17	Human Milk Lactose	0:17	Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding.					
34835980	7	25	theme	maternal	746:753	arg1	measures					758:765	Higher maternal BC measures	739:765	Higher maternal BC measures	739:765	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34835980	8	26	theme	components	953:962	arg1	concentrations					924:937	concentrations	924:937	concentrations	924:937	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	8	26	theme	components	953:962	arg1	intake					979:984	24-h milk intake	969:984	24-h milk intake	969:984	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	8	26	theme	components	953:962	arg1	CDI					943:945	CDI	943:945	CDI of HM components	943:962	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	9	27	theme	HM	1017:1018	arg1	intake					1007:1012	24-h intake	1002:1012	24-h intake of HM and lactose as well as maternal adiposity	1002:1060	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	9	27	theme	HM	1017:1018	arg1	adiposity					1052:1060	maternal adiposity	1043:1060	24-h intake of HM and lactose as well as maternal adiposity	1002:1060	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	9	28	from	related	1066:1072	arg1	conclusion					990:999	conclusion	990:999	conclusion	990:999	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	2	29	theme	milk	369:372	arg1	intake					374:379	24-h milk intake	364:379	24-h milk intake	364:379	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	7	30	theme	BC	755:756	arg1	measures					758:765	Higher maternal BC measures	739:765	Higher maternal BC measures	739:765	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34835980	6	31	theme	body	714:717	arg1	anthropometry					694:706	infant anthropometry	687:706	infant anthropometry	687:706	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	6	31	theme	body	714:717	arg1	mass					719:722	lean body mass	709:722	lean body mass	709:722	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	9	32	theme	infant	1092:1097	arg1	BC					1099:1100	infant BC	1092:1100	infant BC	1092:1100	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	6	33	theme	milk	624:627	arg1	intake					629:634	24-h milk intake	619:634	24-h milk intake	619:634	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	2	34	theme	24-h	364:367	arg1	intake					374:379	24-h milk intake	364:379	24-h milk intake	364:379	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	35	theme	HM	268:269	arg1	lactose					271:277	HM lactose	268:277	HM lactose	268:277	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	6	36	theme	24-h	619:622	arg1	intake					629:634	24-h milk intake	619:634	24-h milk intake	619:634	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	3	37	theme	HM	421:422	arg1	samples					424:430	HM samples	421:430	HM samples	421:430	HM samples were collected at 2 months postpartum.					
34835980	8	38	theme	Maternal	857:864	arg1	characteristics					866:880	Maternal characteristics	857:880	Maternal characteristics including BC and age	857:901	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	8	38	theme	Maternal	857:864	arg1	age					899:901	age	899:901	age	899:901	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	8	38	theme	Maternal	857:864	arg1	BC					892:893	BC	892:893	BC	892:893	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	9	39	from	conclusion	990:999	arg1	related					1066:1072	related	1066:1072	related	1066:1072	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	6	40	theme	lean	709:712	arg1	anthropometry					694:706	infant anthropometry	687:706	infant anthropometry	687:706	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	6	40	theme	lean	709:712	arg1	mass					719:722	lean body mass	709:722	lean body mass	709:722	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	7	41	theme	Higher	739:744	arg1	measures					758:765	Higher maternal BC measures	739:765	Higher maternal BC measures	739:765	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34835980	2	42	dep	lactose	271:277	arg1	CDI					347:349	CDI	347:349	CDI	347:349	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	42	dep	lactose	271:277	arg1	intakes					338:344	calculated daily intakes	321:344	calculated daily intakes (CDI)	321:350	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	42	dep	lactose	271:277	arg1	concentrations					302:315	concentrations	302:315	concentrations	302:315	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	43	theme	maternal	385:392	arg1	BC					394:395	maternal BC	385:395	maternal BC	385:395	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	9	44	theme	maternal	1043:1050	arg1	adiposity					1052:1060	maternal adiposity	1043:1060	24-h intake of HM and lactose as well as maternal adiposity	1002:1060	In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.					
34835980	1	45	theme	Human	109:113	arg1	milk					115:118	Human milk	109:118	Human milk (HM) components	109:134	Human milk (HM) components may influence infant growth and development.					
34835980	1	45	theme	Human	109:113	arg1	HM					121:122	HM	121:122	HM	121:122	Human milk (HM) components may influence infant growth and development.					
34835980	8	46	theme	HM	950:951	arg1	components					953:962	HM components	950:962	HM components	950:962	Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake.					
34835980	6	47	theme	infant	687:692	arg1	anthropometry					694:706	infant anthropometry	687:706	infant anthropometry	687:706	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	6	47	theme	infant	687:692	arg1	adiposity					728:736	adiposity	728:736	adiposity	728:736	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	6	47	theme	infant	687:692	arg1	mass					719:722	lean body mass	709:722	lean body mass	709:722	24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity.					
34835980	1	48	theme	milk	115:118	arg1	components					125:134	Human milk (HM) components	109:134	Human milk (HM) components	109:134	Human milk (HM) components may influence infant growth and development.					
34835980	0	49	dep	Lactose	11:17	arg1	Composition					65:75	Infant Body Composition	53:75	Infant Body Composition during Exclusive Breastfeeding	53:106	Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding.					
34835980	2	50	theme	infant	235:240	arg1	BC					260:261	BC	260:261	BC	260:261	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	50	theme	infant	235:240	arg1	composition					247:257	infant body composition	235:257	infant body composition (BC)	235:262	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	51	theme	daily	332:336	arg1	CDI					347:349	CDI	347:349	CDI	347:349	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	51	theme	daily	332:336	arg1	intakes					338:344	calculated daily intakes	321:344	calculated daily intakes (CDI)	321:350	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	52	theme	calculated	321:330	arg1	CDI					347:349	CDI	347:349	CDI	347:349	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	2	52	theme	calculated	321:330	arg1	intakes					338:344	calculated daily intakes	321:344	calculated daily intakes (CDI)	321:350	This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum.					
34835980	7	53	theme	lean	826:829	arg1	mass					836:839	lean body mass	826:839	lean body mass	826:839	Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity.					
34533763	6	0	dep	candidates	1319:1328	arg1	used					1403:1406	used	1403:1406	could be used	1394:1406	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	5	1	theme	cell	1052:1055	arg1	BCCs					1068:1071	BCCs	1068:1071	BCCs	1068:1071	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	1	theme	cell	1052:1055	arg1	carcinoma					1057:1065	basal cell carcinoma	1046:1065	namely basal cell carcinoma (BCCs)	1039:1072	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	1	theme	cell	1052:1055	arg1	cells					1032:1036	cutaneous progenitor cells	1011:1036	cutaneous progenitor cells	1011:1036	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	2	2	theme	fibroin-soluble	409:423	arg1	scaffolds					469:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	8	3	theme	control	1703:1709	arg1	group					1711:1715	control group	1703:1715	control group	1703:1715	Results revealed that chitosan-containing nanofibrous scaffolds indicate inhibition against S. aureus, but PCL-SF-SESM as control group not.					
34533763	3	4	theme	contact	757:763	arg1	tests					771:775	water contact angle tests	751:775	water contact angle tests	751:775	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	6	5	dep	in	1373:1374	arg1	vivo					1376:1379	vivo	1376:1379	vivo	1376:1379	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	2	6	theme	caprolactone-silk	391:407	arg1	scaffolds					469:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	5	7	theme	basal	1046:1050	arg1	BCCs					1068:1071	BCCs	1068:1071	BCCs	1068:1071	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	7	theme	basal	1046:1050	arg1	carcinoma					1057:1065	basal cell carcinoma	1046:1065	namely basal cell carcinoma (BCCs)	1039:1072	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	7	theme	basal	1046:1050	arg1	cells					1032:1036	cutaneous progenitor cells	1011:1036	cutaneous progenitor cells	1011:1036	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	0	8	theme	tissue	106:111	arg1	engineering					113:123	cutaneous tissue engineering	96:123	cutaneous tissue engineering	96:123	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	3	9	theme	angle	765:769	arg1	tests					771:775	water contact angle tests	751:775	water contact angle tests	751:775	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	6	10	theme	cutaneous	1334:1342	arg1	regeneration					1344:1355	cutaneous regeneration	1334:1355	cutaneous regeneration	1334:1355	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	1	11	used	used	181:184	arg2	scaffolds					154:162	polymeric nanofibrous scaffolds	132:162	polymeric nanofibrous scaffolds	132:162	Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected.					
34533763	5	12	theme	progenitor	1021:1030	arg1	carcinoma					1057:1065	basal cell carcinoma	1046:1065	namely basal cell carcinoma (BCCs)	1039:1072	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	12	theme	progenitor	1021:1030	arg1	cells					1032:1036	cutaneous progenitor cells	1011:1036	cutaneous progenitor cells	1011:1036	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	0	13	theme	cutaneous	96:104	arg1	engineering					113:123	cutaneous tissue engineering	96:123	cutaneous tissue engineering	96:123	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	3	14	dep	Fourier	666:672	arg1	transform					674:682	transform	674:682	transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests	674:775	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	5	15	theme	differentiation	984:998	arg1	process					1000:1006	differentiation process	984:1006	differentiation process	984:1006	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	16	theme	Immunofluorescence	1147:1164	arg1	imaging					1166:1172	Immunofluorescence imaging	1147:1172	Immunofluorescence imaging (IF)	1147:1177	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	4	17	theme	Diffraction	913:923	arg1	patterns					931:938	X-Ray Diffraction (XRD) patterns	907:938	X-Ray Diffraction (XRD) patterns	907:938	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	9	18	located	observed	1787:1794	arg1	addition					1725:1732	addition	1725:1732	addition	1725:1732	In addition, against C. albicans any antifungal activity was not observed.					
34533763	9	18	located	observed	1787:1794	arg2	activity					1770:1777	any antifungal activity	1755:1777	any antifungal activity	1755:1777	In addition, against C. albicans any antifungal activity was not observed.					
34533763	3	19	dep	infrared	684:691	arg1	FT-IR					694:698	FT-IR	694:698	FT-IR	694:698	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	4	20	theme	X-Ray	907:911	arg1	patterns					931:938	X-Ray Diffraction (XRD) patterns	907:938	X-Ray Diffraction (XRD) patterns	907:938	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	2	21	theme	present	275:281	arg1	work					283:286	the present work	271:286	the present work	271:286	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	1	22	theme	tissue	189:194	arg1	regeneration					196:207	tissue regeneration	189:207	tissue regeneration	189:207	Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected.					
34533763	2	23	theme	cutaneous	530:538	arg1	regeneration					540:551	cutaneous regeneration	530:551	cutaneous regeneration	530:551	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	4	24	theme	XRD	926:928	arg1	patterns					931:938	X-Ray Diffraction (XRD) patterns	907:938	X-Ray Diffraction (XRD) patterns	907:938	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	4	25	theme	PCL/SF/SESM/AgNPs	804:820	arg1	nanofibers					822:831	PCL/SF/SESM/AgNPs nanofibers	804:831	PCL/SF/SESM/AgNPs nanofibers	804:831	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	2	26	theme	PCL-SF-SESM-CS	453:466	arg1	scaffolds					469:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	4	27	theme	Transmission	862:873	arg1	microscopes					884:894	Transmission electron microscopes	862:894	Transmission electron microscopes (TEM)	862:900	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	4	27	theme	Transmission	862:873	arg1	TEM					897:899	TEM	897:899	TEM	897:899	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	8	28	theme	chitosan-containing	1603:1621	arg1	scaffolds					1635:1643	chitosan-containing nanofibrous scaffolds	1603:1643	chitosan-containing nanofibrous scaffolds	1603:1643	Results revealed that chitosan-containing nanofibrous scaffolds indicate inhibition against S. aureus, but PCL-SF-SESM as control group not.					
34533763	2	29	theme	membrane-silver	337:351	arg1	PCL-SF-SESM-AgNPs					368:384	PCL-SF-SESM-AgNPs	368:384	PCL-SF-SESM-AgNPs	368:384	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	2	29	theme	membrane-silver	337:351	arg1	nanoparticles					353:365	poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles	289:365	poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs)	289:385	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	9	30	theme	antifungal	1759:1768	arg1	activity					1770:1777	any antifungal activity	1755:1777	any antifungal activity	1755:1777	In addition, against C. albicans any antifungal activity was not observed.					
34533763	9	31	theme	C.	1743:1744	arg1	albicans					1746:1753	C. albicans	1743:1753	C. albicans	1743:1753	In addition, against C. albicans any antifungal activity was not observed.					
34533763	2	32	theme	eggshell	328:335	arg1	PCL-SF-SESM-AgNPs					368:384	PCL-SF-SESM-AgNPs	368:384	PCL-SF-SESM-AgNPs	368:384	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	2	32	theme	eggshell	328:335	arg1	nanoparticles					353:365	poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles	289:365	poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs)	289:385	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	5	33	theme	cells	1032:1036	arg1	adhesion					955:962	cell adhesion	950:962	cell adhesion	950:962	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	33	theme	cells	1032:1036	arg1	process					1000:1006	differentiation process	984:1006	differentiation process	984:1006	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	33	theme	cells	1032:1036	arg1	proliferation					965:977	proliferation	965:977	proliferation	965:977	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	2	34	theme	electrospinning	503:517	arg1	method					519:524	the electrospinning method	499:524	the electrospinning method for cutaneous regeneration	499:551	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	5	35	theme	MTT	1127:1129	arg1	analysis					1131:1138	MTT analysis	1127:1138	MTT analysis	1127:1138	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	2	36	theme	fibroin-soluble	312:326	arg1	PCL-SF-SESM-AgNPs					368:384	PCL-SF-SESM-AgNPs	368:384	PCL-SF-SESM-AgNPs	368:384	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	2	36	theme	fibroin-soluble	312:326	arg1	nanoparticles					353:365	poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles	289:365	poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs)	289:385	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	3	37	dep	transform	674:682	arg1	infrared					684:691	infrared	684:691	infrared	684:691	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	38	theme	mechanical	603:612	arg1	features					614:621	mechanical features	603:621	mechanical features	603:621	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	0	39	theme	antibacterial	35:47	arg1	properties					49:58	antibacterial properties	35:58	antibacterial properties	35:58	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	8	40	theme	nanofibrous	1623:1633	arg1	scaffolds					1635:1643	chitosan-containing nanofibrous scaffolds	1603:1643	chitosan-containing nanofibrous scaffolds	1603:1643	Results revealed that chitosan-containing nanofibrous scaffolds indicate inhibition against S. aureus, but PCL-SF-SESM as control group not.					
34533763	6	41	theme	nanofibrous	1286:1296	arg1	mats					1298:1301	prepared nanofibrous mats	1277:1301	prepared nanofibrous mats	1277:1301	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	6	41	theme	nanofibrous	1286:1296	arg1	candidates					1319:1328	appropriate candidates	1307:1328	appropriate candidates for cutaneous regeneration	1307:1355	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	7	42	theme	diffusion	1563:1571	arg1	method					1573:1578	the disc diffusion method	1554:1578	the disc diffusion method	1554:1578	Lastly, the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans was analyzed using the disc diffusion method.					
34533763	6	43	theme	appropriate	1307:1317	arg1	mats					1298:1301	prepared nanofibrous mats	1277:1301	prepared nanofibrous mats	1277:1301	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	6	43	theme	appropriate	1307:1317	arg1	candidates					1319:1328	appropriate candidates	1307:1328	appropriate candidates for cutaneous regeneration	1307:1355	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	5	44	theme	Real-Time	1184:1192	arg1	QRT-PCR					1234:1240	QRT-PCR	1234:1240	QRT-PCR	1234:1240	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	44	theme	Real-Time	1184:1192	arg1	PCR					1229:1231	Real-Time Quantitative Reverse Transcription PCR	1184:1231	Real-Time Quantitative Reverse Transcription PCR (QRT-PCR)	1184:1241	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	7	45	theme	nanofibers	1457:1466	arg1	potential					1435:1443	the antimicrobial potential	1417:1443	the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans	1417:1533	Lastly, the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans was analyzed using the disc diffusion method.					
34533763	6	46	theme	prepared	1277:1284	arg1	mats					1298:1301	prepared nanofibrous mats	1277:1301	prepared nanofibrous mats	1277:1301	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	6	46	theme	prepared	1277:1284	arg1	candidates					1319:1328	appropriate candidates	1307:1328	appropriate candidates for cutaneous regeneration	1307:1355	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	1	47	theme	bacterial	222:230	arg1	infections					232:241	bacterial infections	222:241	bacterial infections	222:241	Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected.					
34533763	5	48	theme	Quantitative	1194:1205	arg1	QRT-PCR					1234:1240	QRT-PCR	1234:1240	QRT-PCR	1234:1240	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	48	theme	Quantitative	1194:1205	arg1	PCR					1229:1231	Real-Time Quantitative Reverse Transcription PCR	1184:1231	Real-Time Quantitative Reverse Transcription PCR (QRT-PCR)	1184:1241	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	8	49	dep	indicate	1645:1652	arg1	scaffolds					1635:1643	chitosan-containing nanofibrous scaffolds	1603:1643	chitosan-containing nanofibrous scaffolds	1603:1643	Results revealed that chitosan-containing nanofibrous scaffolds indicate inhibition against S. aureus, but PCL-SF-SESM as control group not.					
34533763	7	50	theme	antimicrobial	1421:1433	arg1	potential					1435:1443	the antimicrobial potential	1417:1443	the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans	1417:1533	Lastly, the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans was analyzed using the disc diffusion method.					
34533763	5	51	theme	Reverse	1207:1213	arg1	QRT-PCR					1234:1240	QRT-PCR	1234:1240	QRT-PCR	1234:1240	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	51	theme	Reverse	1207:1213	arg1	PCR					1229:1231	Real-Time Quantitative Reverse Transcription PCR	1184:1231	Real-Time Quantitative Reverse Transcription PCR (QRT-PCR)	1184:1241	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	6	52	theme	in	1373:1374	arg1	applications					1381:1392	advanced in vivo applications	1364:1392	advanced in vivo applications	1364:1392	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	7	53	theme	disc	1558:1561	arg1	method					1573:1578	the disc diffusion method	1554:1578	the disc diffusion method	1554:1578	Lastly, the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans was analyzed using the disc diffusion method.					
34533763	5	54	theme	keratinocyte-like	1082:1098	arg1	cells					1100:1104	keratinocyte-like cells	1082:1104	keratinocyte-like cells	1082:1104	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	1	55	theme	polymeric	132:140	arg1	scaffolds					154:162	polymeric nanofibrous scaffolds	132:162	polymeric nanofibrous scaffolds	132:162	Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected.					
34533763	4	56	theme	AgNPs	795:799	arg1	existence					782:790	The existence	778:790	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers	778:831	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	5	57	theme	Transcription	1215:1227	arg1	QRT-PCR					1234:1240	QRT-PCR	1234:1240	QRT-PCR	1234:1240	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	57	theme	Transcription	1215:1227	arg1	PCR					1229:1231	Real-Time Quantitative Reverse Transcription PCR	1184:1231	Real-Time Quantitative Reverse Transcription PCR (QRT-PCR)	1184:1241	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	6	58	theme	advanced	1364:1371	arg1	applications					1381:1392	advanced in vivo applications	1364:1392	advanced in vivo applications	1364:1392	The results indicated that prepared nanofibrous mats are appropriate candidates for cutaneous regeneration and in advanced in vivo applications could be used.					
34533763	3	59	theme	water	751:755	arg1	tests					771:775	water contact angle tests	751:775	water contact angle tests	751:775	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	60	theme	electron	711:718	arg1	SEM					732:734	SEM	732:734	SEM	732:734	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	60	theme	electron	711:718	arg1	microscope					720:729	scanning electron microscope	702:729	scanning electron microscope (SEM)	702:735	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	7	61	theme	prepared	1448:1455	arg1	nanofibers					1457:1466	prepared nanofibers	1448:1466	prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans	1448:1533	Lastly, the antimicrobial potential of prepared nanofibers against microorganisms such as E. coli, S. aureus, and C. Albicans was analyzed using the disc diffusion method.					
34533763	1	62	theme	nanofibrous	142:152	arg1	scaffolds					154:162	polymeric nanofibrous scaffolds	132:162	polymeric nanofibrous scaffolds	132:162	Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected.					
34533763	5	63	theme	cutaneous	1011:1019	arg1	carcinoma					1057:1065	basal cell carcinoma	1046:1065	namely basal cell carcinoma (BCCs)	1039:1072	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	5	63	theme	cutaneous	1011:1019	arg1	cells					1032:1036	cutaneous progenitor cells	1011:1036	cutaneous progenitor cells	1011:1036	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	1	64	theme	infections	232:241	arg1	risk					214:217	the risk	210:217	the risk of bacterial infections	210:241	Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected.					
34533763	3	65	theme	prepared	626:633	arg1	scaffolds					635:643	prepared scaffolds	626:643	prepared scaffolds	626:643	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	66	theme	scaffolds	635:643	arg1	hydrophilicity					583:596	hydrophilicity	583:596	hydrophilicity	583:596	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	66	theme	scaffolds	635:643	arg1	features					614:621	mechanical features	603:621	mechanical features	603:621	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	66	theme	scaffolds	635:643	arg1	morphology					571:580	morphology	571:580	morphology	571:580	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	66	theme	scaffolds	635:643	arg1	composition					558:568	The composition	554:568	The composition	554:568	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	8	67	dep	revealed	1589:1596	arg1	indicate					1645:1652	indicate	1645:1652	indicate inhibition against S. aureus, but PCL-SF-SESM as control group not	1645:1719	Results revealed that chitosan-containing nanofibrous scaffolds indicate inhibition against S. aureus, but PCL-SF-SESM as control group not.					
34533763	5	68	theme	cell	950:953	arg1	adhesion					955:962	cell adhesion	950:962	cell adhesion	950:962	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34533763	4	69	from	existence	782:790	arg1	nanofibers					822:831	PCL/SF/SESM/AgNPs nanofibers	804:831	PCL/SF/SESM/AgNPs nanofibers	804:831	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	4	70	theme	electron	875:882	arg1	microscopes					884:894	Transmission electron microscopes	862:894	Transmission electron microscopes (TEM)	862:900	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	4	70	theme	electron	875:882	arg1	TEM					897:899	TEM	897:899	TEM	897:899	The existence of AgNPs in PCL/SF/SESM/AgNPs nanofibers was confirmed by UV-visible, Transmission electron microscopes (TEM), and X-Ray Diffraction (XRD) patterns.					
34533763	0	71	theme	nanofibrous	70:80	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	0	71	theme	nanofibrous	70:80	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	0	71	theme	nanofibrous	70:80	arg1	properties					49:58	antibacterial properties	35:58	antibacterial properties	35:58	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	2	72	theme	membrane-chitosan	434:450	arg1	scaffolds					469:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	0	73	theme	hybrid	63:68	arg1	nanofibrous					70:80	hybrid nanofibrous	63:80	hybrid nanofibrous	63:80	Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.					
34533763	3	74	theme	scanning	702:709	arg1	SEM					732:734	SEM	732:734	SEM	732:734	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	3	74	theme	scanning	702:709	arg1	microscope					720:729	scanning electron microscope	702:729	scanning electron microscope (SEM)	702:735	The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests.					
34533763	2	75	theme	eggshell	425:432	arg1	scaffolds					469:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds	391:477	In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration.					
34533763	5	76	dep	analysis	1131:1138	arg1	assay					1243:1247	assay	1243:1247	assay	1243:1247	Besides, cell adhesion, proliferation, and differentiation process of cutaneous progenitor cells, namely basal cell carcinoma (BCCs), toward keratinocyte-like cells were evaluated using MTT analysis, DAPI, Immunofluorescence imaging (IF), and Real-Time Quantitative Reverse Transcription PCR (QRT-PCR) assay.					
34021830	6	0	theme	medium	1364:1369	arg1	optimization					1371:1382	rational medium optimization	1355:1382	rational medium optimization	1355:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	2	1	theme	tacrolimus	693:702	arg1	production					704:713	tacrolimus production	693:713	tacrolimus production	693:713	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	5	2	theme	3.7	1259:1261	arg1	fermenter					1232:1240	a 1000 L fermenter	1223:1240	a 1000 L fermenter	1223:1240	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	5	2	theme	3.7	1259:1261	arg1	times					1263:1267	times	1263:1267	times	1263:1267	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	2	3	theme	S.	408:409	arg1	FIM-16-06					424:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	2	4	theme	soluble	626:632	arg1	starch					634:639	soluble starch	626:639	soluble starch	626:639	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	6	5	theme	effective	1390:1398	arg1	mutation					1341:1348	CONCLUSIONS Combining compound mutation	1310:1348	CONCLUSIONS Combining compound mutation with rational medium optimization	1310:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	5	theme	effective	1390:1398	arg1	approach					1400:1407	an effective approach	1387:1407	an effective approach for improving tacrolimus production	1387:1443	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	2	6	theme	strain	401:406	arg1	FIM-16-06					424:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	4	7	theme	soluble	991:997	arg1	starch					999:1004	soluble starch	991:1004	soluble starch	991:1004	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	0	8	theme	medium	114:119	arg1	optimization					85:96	optimization	85:96	optimization	85:96	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	0	8	theme	medium	114:119	arg1	mutagenesis					69:79	mutagenesis	69:79	mutagenesis	69:79	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	0	9	from	Improvement	0:10	arg1	tsukubaensis					53:64	Streptomyces tsukubaensis	40:64	Streptomyces tsukubaensis	40:64	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	2	10	dep	S.	408:409	arg1	tsukubaensis					411:422	tsukubaensis	411:422	tsukubaensis	411:422	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	1	11	theme	strain	301:306	arg1	mutagenesis					308:318	strain mutagenesis	301:318	strain mutagenesis	301:318	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	0	12	theme	fermentation	101:112	arg1	medium					114:119	fermentation medium	101:119	fermentation medium	101:119	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	4	13	theme	starch	999:1004	arg1	20.61 g/L					1007:1015	20.61 g/L	1007:1015	20.61 g/L of peptone	1007:1026	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	13	theme	starch	999:1004	arg1	61.61 g/L					978:986	61.61 g/L	978:986	61.61 g/L of soluble starch	978:1004	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	13	theme	starch	999:1004	arg1	30.79 g/L					1032:1040	30.79 g/L	1032:1040	30.79 g/L of Tween 80	1032:1052	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	0	14	theme	Plackett-Burman	127:141	arg1	design					143:148	Plackett-Burman design	127:148	Plackett-Burman design combined with response surface methodology	127:191	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	5	15	dep	times	1263:1267	arg1	higher					1269:1274	higher	1269:1274	higher	1269:1274	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	6	16	theme	tacrolimus	1423:1432	arg1	production					1434:1443	tacrolimus production	1423:1443	tacrolimus production	1423:1443	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	2	17	theme	ultraviolet	450:460	arg1	mutagenesis					462:472	ultraviolet mutagenesis	450:472	ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then	450:542	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	2	18	theme	experimental	596:607	arg1	design					609:614	Plackett-Burman experimental design	580:614	Plackett-Burman experimental design	580:614	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	5	19	theme	L	1230:1230	arg1	fermenter					1232:1240	a 1000 L fermenter	1223:1240	a 1000 L fermenter	1223:1240	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	5	19	theme	L	1230:1230	arg1	times					1263:1267	times	1263:1267	times	1263:1267	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	2	20	dep	RESULTS	365:371	arg1	obtained					438:445	obtained	438:445	was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then	434:542	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	2	21	theme	plasma	520:525	arg1	mutagenesis.Then					527:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	6	22	theme	rational	1355:1362	arg1	optimization					1371:1382	rational medium optimization	1355:1382	rational medium optimization	1355:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	23	theme	industrial	1514:1523	arg1	production					1525:1534	industrial production	1514:1534	industrial production	1514:1534	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	24	theme	compound	1332:1339	arg1	approach					1400:1407	an effective approach	1387:1407	an effective approach for improving tacrolimus production	1387:1443	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	24	theme	compound	1332:1339	arg1	mutation					1341:1348	CONCLUSIONS Combining compound mutation	1310:1348	CONCLUSIONS Combining compound mutation with rational medium optimization	1310:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	25	used	used	1505:1508	arg2	medium					1477:1482	the optimized fermentation medium	1450:1482	the optimized fermentation medium	1450:1482	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	2	26	theme	temperature	508:518	arg1	mutagenesis.Then					527:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	1	27	dep	OBJECTIVE	194:202	arg1	conducted					219:227	conducted	219:227	was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium	215:362	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	0	28	theme	production	26:35	arg1	Improvement					0:10	Improvement	0:10	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium	0:119	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	3	29	theme	optimal	858:864	arg1	composition					886:896	optimal fermentation medium composition	858:896	optimal fermentation medium composition for tacrolimus production	858:922	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	6	30	theme	optimized	1454:1462	arg1	medium					1477:1482	the optimized fermentation medium	1450:1482	the optimized fermentation medium	1450:1482	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	2	31	theme	atmospheric	487:497	arg1	mutagenesis.Then					527:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	0	32	theme	tacrolimus	15:24	arg1	production					26:35	tacrolimus production	15:35	tacrolimus production	15:35	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	5	33	theme	optimized	1062:1070	arg1	medium					1072:1077	the optimized medium	1058:1077	the optimized medium	1058:1077	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	0	34	theme	response	164:171	arg1	methodology					181:191	response surface methodology	164:191	response surface methodology	164:191	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	5	35	theme	scaled-up	1183:1191	arg1	fermentation					1193:1204	the scaled-up fermentation	1179:1204	the scaled-up fermentation	1179:1204	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	4	36	theme	fermentation	937:948	arg1	medium					950:955	The optimum fermentation medium	925:955	The optimum fermentation medium	925:955	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	5	37	theme	original	1293:1300	arg1	medium					1302:1307	the original medium	1289:1307	the original medium	1289:1307	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	2	38	dep	showed	663:668	arg1	affected					684:691	affected	684:691	showed significantly affected tacrolimus production	663:713	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	1	39	theme	fermentation	344:355	arg1	medium					357:362	the fermentation medium	340:362	the fermentation medium	340:362	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	4	40	theme	Tween	1045:1049	arg1	20.61 g/L					1007:1015	20.61 g/L	1007:1015	20.61 g/L of peptone	1007:1026	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	40	theme	Tween	1045:1049	arg1	61.61 g/L					978:986	61.61 g/L	978:986	61.61 g/L of soluble starch	978:1004	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	40	theme	Tween	1045:1049	arg1	30.79 g/L					1032:1040	30.79 g/L	1032:1040	30.79 g/L of Tween 80	1032:1052	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	2	41	theme	room	503:506	arg1	mutagenesis.Then					527:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	atmospheric and room temperature plasma mutagenesis.Then	487:542	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	6	42	theme	fermentation	1464:1475	arg1	medium					1477:1482	the optimized fermentation medium	1450:1482	the optimized fermentation medium	1450:1482	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	1	43	theme	medium	357:362	arg1	optimization					324:335	optimization	324:335	optimization	324:335	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	1	43	theme	medium	357:362	arg1	mutagenesis					308:318	strain mutagenesis	301:318	strain mutagenesis	301:318	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	6	44	theme	Combining	1322:1330	arg1	approach					1400:1407	an effective approach	1387:1407	an effective approach for improving tacrolimus production	1387:1443	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	44	theme	Combining	1322:1330	arg1	mutation					1341:1348	CONCLUSIONS Combining compound mutation	1310:1348	CONCLUSIONS Combining compound mutation with rational medium optimization	1310:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	1	45	from	production	244:253	arg1	tsukubaensis					285:296	Streptomyces tsukubaensis	272:296	Streptomyces tsukubaensis	272:296	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	2	46	theme	producing	391:399	arg1	FIM-16-06					424:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	3	47	theme	fermentation	866:877	arg1	composition					886:896	optimal fermentation medium composition	858:896	optimal fermentation medium composition for tacrolimus production	858:922	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	0	48	theme	surface	173:179	arg1	methodology					181:191	response surface methodology	164:191	response surface methodology	164:191	Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett-Burman design combined with response surface methodology.					
34021830	6	49	theme	CONCLUSIONS	1310:1320	arg1	approach					1400:1407	an effective approach	1387:1407	an effective approach for improving tacrolimus production	1387:1443	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	6	49	theme	CONCLUSIONS	1310:1320	arg1	mutation					1341:1348	CONCLUSIONS Combining compound mutation	1310:1348	CONCLUSIONS Combining compound mutation with rational medium optimization	1310:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	2	50	theme	tacrolimus	380:389	arg1	FIM-16-06					424:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	4	51	contain	contain	970:976	arg2	61.61 g/L					978:986	61.61 g/L	978:986	61.61 g/L of soluble starch	978:1004	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	51	contain	contain	970:976	arg1	medium					950:955	The optimum fermentation medium	925:955	The optimum fermentation medium	925:955	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	51	contain	contain	970:976	arg2	30.79 g/L					1032:1040	30.79 g/L	1032:1040	30.79 g/L of Tween 80	1032:1052	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	51	contain	contain	970:976	arg2	20.61 g/L					1007:1015	20.61 g/L	1007:1015	20.61 g/L of peptone	1007:1026	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	3	52	theme	medium	879:884	arg1	composition					886:896	optimal fermentation medium composition	858:896	optimal fermentation medium composition for tacrolimus production	858:922	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	5	53	theme	shake-flask	1130:1140	arg1	culture					1142:1148	shake-flask culture	1130:1148	shake-flask culture	1130:1148	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	3	54	theme	Further	716:722	arg1	studies					724:730	Further studies	716:730	Further studies	716:730	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	4	55	theme	optimum	929:935	arg1	medium					950:955	The optimum fermentation medium	925:955	The optimum fermentation medium	925:955	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	2	56	theme	high	375:378	arg1	FIM-16-06					424:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	A high tacrolimus producing strain S. tsukubaensis FIM-16-06	373:432	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	4	57	theme	peptone	1020:1026	arg1	20.61 g/L					1007:1015	20.61 g/L	1007:1015	20.61 g/L of peptone	1007:1026	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	57	theme	peptone	1020:1026	arg1	61.61 g/L					978:986	61.61 g/L	978:986	61.61 g/L of soluble starch	978:1004	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	4	57	theme	peptone	1020:1026	arg1	30.79 g/L					1032:1040	30.79 g/L	1032:1040	30.79 g/L of Tween 80	1032:1052	The optimum fermentation medium was found to contain 61.61 g/L of soluble starch, 20.61 g/L of peptone and 30.79 g/L of Tween 80.					
34021830	3	58	theme	central	759:765	arg1	design					777:782	central composite design	759:782	central composite design	759:782	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	5	59	theme	tacrolimus	1098:1107	arg1	production					1084:1093	the production	1080:1093	the production of tacrolimus	1080:1107	In the optimized medium, the production of tacrolimus reached 1293 mg/L in shake-flask culture, and reached 1522 mg/L while the scaled-up fermentation was conducted in a 1000 L fermenter, which was about 3.7 times higher than that in the original medium.					
34021830	1	60	theme	tacrolimus	258:267	arg1	production					244:253	the production	240:253	the production of tacrolimus in Streptomyces tsukubaensis	240:296	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
34021830	2	61	theme	Plackett-Burman	580:594	arg1	design					609:614	Plackett-Burman experimental design	580:614	Plackett-Burman experimental design	580:614	RESULTS A high tacrolimus producing strain S. tsukubaensis FIM-16-06 was obtained by ultraviolet mutagenesis coupled with atmospheric and room temperature plasma mutagenesis.Then, nine variables were screened using Plackett-Burman experimental design, in which soluble starch, peptone and Tween 80 showed significantly affected tacrolimus production.					
34021830	3	62	theme	composite	767:775	arg1	design					777:782	central composite design	759:782	central composite design	759:782	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	3	63	theme	tacrolimus	902:911	arg1	production					913:922	tacrolimus production	902:922	tacrolimus production	902:922	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	3	64	theme	mutual	801:806	arg1	interaction					808:818	the mutual interaction	797:818	the mutual interaction between the variables	797:840	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	3	65	dep	employing	749:757	arg1	elucidate					787:795	elucidate	787:795	to elucidate the mutual interaction between the variables	784:840	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	3	65	dep	employing	749:757	arg1	work					849:852	work	849:852	to work out optimal fermentation medium composition for tacrolimus production	846:922	Further studies were carried out employing central composite design to elucidate the mutual interaction between the variables and to work out optimal fermentation medium composition for tacrolimus production.					
34021830	6	66	with	mutation	1341:1348	arg1	optimization					1371:1382	rational medium optimization	1355:1382	rational medium optimization	1355:1382	CONCLUSIONS Combining compound mutation with rational medium optimization is an effective approach for improving tacrolimus production, and the optimized fermentation medium could be efficiently used for industrial production.					
34021830	1	67	theme	Streptomyces	272:283	arg1	tsukubaensis					285:296	Streptomyces tsukubaensis	272:296	Streptomyces tsukubaensis	272:296	OBJECTIVE This study was conducted to enhance the production of tacrolimus in Streptomyces tsukubaensis by strain mutagenesis and optimization of the fermentation medium.					
32061698	1	0	theme	inorganic-protein	165:181	arg1	β-galactosidase					110:124	Thermostable β-galactosidase	97:124	Thermostable β-galactosidase (TmLac)	97:132	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	0	theme	inorganic-protein	165:181	arg1	nanoflowers					183:193	hybrid inorganic-protein nanoflowers	158:193	hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component	158:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	4	1	theme	equivalent	572:581	arg1	level					546:550	a level	544:550	a level of lactase activity equivalent to their same content of free enzyme	544:618	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	0	2	theme	lactose	88:94	arg1	hydrolysis					69:78	the hydrolysis	65:78	the hydrolysis of milk lactose	65:94	Recyclable thermophilic hybrid protein-inorganic nanoflowers for the hydrolysis of milk lactose.					
32061698	4	3	theme	Mn2+	511:514	arg1	nanoflowers					525:535	Ca2+, Mn2+ and Co2+ nanoflowers	505:535	Ca2+, Mn2+ and Co2+ nanoflowers	505:535	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	5	4	theme	marginal	649:656	arg1	activity					665:672	only marginal enzyme activity	644:672	only marginal enzyme activity	644:672	Cu2+nanoflowers showed only marginal enzyme activity in agreement with the inhibitory effect of this cation on the enzyme.					
32061698	7	5	theme	lactose	897:903	arg1	hydrolysis					905:914	lactose hydrolysis	897:914	lactose hydrolysis	897:914	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	4	6	theme	activity	563:570	arg1	level					546:550	a level	544:550	a level of lactase activity equivalent to their same content of free enzyme	544:618	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	5	7	theme	cation	722:727	arg1	effect					707:712	the inhibitory effect	692:712	the inhibitory effect of this cation on the enzyme	692:741	Cu2+nanoflowers showed only marginal enzyme activity in agreement with the inhibitory effect of this cation on the enzyme.					
32061698	5	8	theme	enzyme	658:663	arg1	activity					665:672	only marginal enzyme activity	644:672	only marginal enzyme activity	644:672	Cu2+nanoflowers showed only marginal enzyme activity in agreement with the inhibitory effect of this cation on the enzyme.					
32061698	7	9	theme	consecutive	974:984	arg1	cycles					986:991	five consecutive cycles	969:991	five consecutive cycles	969:991	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	1	10	theme	inorganic	249:257	arg1	salts					201:205	salts	201:205	salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+	201:240	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	10	theme	inorganic	249:257	arg1	component					259:267	the inorganic component	245:267	the inorganic component	245:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	4	11	theme	enzyme	613:618	arg1	content					597:603	their same content	586:603	their same content of free enzyme	586:618	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	2	12	theme	incorporation	274:286	arg1	higher					334:339	higher	334:339	higher	334:339	The incorporation efficiency of enzyme into the nanoflowers was higher than 95% for a protein concentration of 0.05 mg/mL.					
32061698	2	12	theme	incorporation	274:286	arg1	efficiency					288:297	The incorporation efficiency	270:297	The incorporation efficiency of enzyme into the nanoflowers	270:328	The incorporation efficiency of enzyme into the nanoflowers was higher than 95% for a protein concentration of 0.05 mg/mL.					
32061698	5	13	from	effect	707:712	arg1	enzyme					736:741	the enzyme	732:741	the enzyme	732:741	Cu2+nanoflowers showed only marginal enzyme activity in agreement with the inhibitory effect of this cation on the enzyme.					
32061698	3	14	theme	different	459:467	arg1	composition					478:488	different chemical composition	459:488	different chemical composition	459:488	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	3	15	with	structure	397:405	arg1	composition					478:488	different chemical composition	459:488	different chemical composition	459:488	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	2	16	theme	0.05 mg/mL	381:390	arg1	concentration					364:376	a protein concentration	354:376	a protein concentration of 0.05 mg/mL	354:390	The incorporation efficiency of enzyme into the nanoflowers was higher than 95% for a protein concentration of 0.05 mg/mL.					
32061698	4	17	theme	same	592:595	arg1	content					597:603	their same content	586:603	their same content of free enzyme	586:618	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	0	18	theme	thermophilic	11:22	arg1	protein-inorganic					31:47	Recyclable thermophilic hybrid protein-inorganic	0:47	Recyclable thermophilic hybrid protein-inorganic	0:47	Recyclable thermophilic hybrid protein-inorganic nanoflowers for the hydrolysis of milk lactose.					
32061698	3	19	dep	structure	397:405	arg1	The					393:395	The	393:395	The	393:395	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	5	20	with	agreement	677:685	arg1	effect					707:712	the inhibitory effect	692:712	the inhibitory effect of this cation on the enzyme	692:741	Cu2+nanoflowers showed only marginal enzyme activity in agreement with the inhibitory effect of this cation on the enzyme.					
32061698	1	21	theme	Thermostable	97:108	arg1	β-galactosidase					110:124	Thermostable β-galactosidase	97:124	Thermostable β-galactosidase (TmLac)	97:132	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	21	theme	Thermostable	97:108	arg1	nanoflowers					183:193	hybrid inorganic-protein nanoflowers	158:193	hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component	158:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	21	theme	Thermostable	97:108	arg1	TmLac					127:131	TmLac	127:131	TmLac	127:131	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	7	22	theme	best	877:880	arg1	properties					882:891	the best properties	873:891	the best properties for lactose hydrolysis	873:914	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	0	23	theme	Recyclable	0:9	arg1	protein-inorganic					31:47	Recyclable thermophilic hybrid protein-inorganic	0:47	Recyclable thermophilic hybrid protein-inorganic	0:47	Recyclable thermophilic hybrid protein-inorganic nanoflowers for the hydrolysis of milk lactose.					
32061698	6	24	theme	efficient	773:781	arg1	methodology					783:793	an efficient methodology	770:793	an efficient methodology for enzyme immobilization and recyclability	770:837	TmLac nanoflowers provide an efficient methodology for enzyme immobilization and recyclability.					
32061698	2	25	theme	protein	356:362	arg1	concentration					364:376	a protein concentration	354:376	a protein concentration of 0.05 mg/mL	354:390	The incorporation efficiency of enzyme into the nanoflowers was higher than 95% for a protein concentration of 0.05 mg/mL.					
32061698	5	26	theme	inhibitory	696:705	arg1	effect					707:712	the inhibitory effect	692:712	the inhibitory effect of this cation on the enzyme	692:741	Cu2+nanoflowers showed only marginal enzyme activity in agreement with the inhibitory effect of this cation on the enzyme.					
32061698	6	27	theme	enzyme	799:804	arg1	immobilization					806:819	enzyme immobilization	799:819	enzyme immobilization	799:819	TmLac nanoflowers provide an efficient methodology for enzyme immobilization and recyclability.					
32061698	2	28	theme	enzyme	302:307	arg1	higher					334:339	higher	334:339	higher	334:339	The incorporation efficiency of enzyme into the nanoflowers was higher than 95% for a protein concentration of 0.05 mg/mL.					
32061698	2	28	theme	enzyme	302:307	arg1	efficiency					288:297	The incorporation efficiency	270:297	The incorporation efficiency of enzyme into the nanoflowers	270:328	The incorporation efficiency of enzyme into the nanoflowers was higher than 95% for a protein concentration of 0.05 mg/mL.					
32061698	0	29	theme	hybrid	24:29	arg1	protein-inorganic					31:47	Recyclable thermophilic hybrid protein-inorganic	0:47	Recyclable thermophilic hybrid protein-inorganic	0:47	Recyclable thermophilic hybrid protein-inorganic nanoflowers for the hydrolysis of milk lactose.					
32061698	4	30	theme	lactase	555:561	arg1	activity					563:570	lactase activity	555:570	lactase activity	555:570	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	7	31	theme	TmLac-Ca2+	840:849	arg1	nanoflowers					851:861	TmLac-Ca2+ nanoflowers	840:861	TmLac-Ca2+ nanoflowers	840:861	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	1	32	theme	Mn2+	216:219	arg1	salts					201:205	salts	201:205	salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+	201:240	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	32	theme	Mn2+	216:219	arg1	component					259:267	the inorganic component	245:267	the inorganic component	245:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	33	theme	Cu2+	210:213	arg1	salts					201:205	salts	201:205	salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+	201:240	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	33	theme	Cu2+	210:213	arg1	component					259:267	the inorganic component	245:267	the inorganic component	245:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	4	34	theme	Co2+	520:523	arg1	nanoflowers					525:535	Ca2+, Mn2+ and Co2+ nanoflowers	505:535	Ca2+, Mn2+ and Co2+ nanoflowers	505:535	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	3	35	theme	nanoflowers	442:452	arg1	recyclability					421:433	recyclability	421:433	recyclability	421:433	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	3	35	theme	nanoflowers	442:452	arg1	activity					408:415	activity	408:415	activity	408:415	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	3	35	theme	nanoflowers	442:452	arg1	structure					397:405	structure	397:405	structure	397:405	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	3	36	with	activity	408:415	arg1	composition					478:488	different chemical composition	459:488	different chemical composition	459:488	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	7	37	attach	presented	863:871	arg1	milk					940:943	milk	940:943	milk	940:943	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	7	37	attach	presented	863:871	arg1	buffered					924:931	buffered	924:931	buffered	924:931	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	7	37	attach	presented	863:871	arg2	nanoflowers					851:861	TmLac-Ca2+ nanoflowers	840:861	TmLac-Ca2+ nanoflowers	840:861	TmLac-Ca2+ nanoflowers presented the best properties for lactose hydrolysis both in buffered and in milk, and could be reused in five consecutive cycles.					
32061698	6	38	theme	TmLac	744:748	arg1	nanoflowers					750:760	TmLac nanoflowers	744:760	TmLac nanoflowers	744:760	TmLac nanoflowers provide an efficient methodology for enzyme immobilization and recyclability.					
32061698	3	39	with	recyclability	421:433	arg1	composition					478:488	different chemical composition	459:488	different chemical composition	459:488	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	3	40	theme	chemical	469:476	arg1	composition					478:488	different chemical composition	459:488	different chemical composition	459:488	The structure, activity and recyclability of the nanoflowers with different chemical composition were analyzed.					
32061698	1	41	theme	Co2+	228:231	arg1	salts					201:205	salts	201:205	salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+	201:240	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	41	theme	Co2+	228:231	arg1	component					259:267	the inorganic component	245:267	the inorganic component	245:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	0	42	theme	milk	83:86	arg1	lactose					88:94	milk lactose	83:94	milk lactose	83:94	Recyclable thermophilic hybrid protein-inorganic nanoflowers for the hydrolysis of milk lactose.					
32061698	1	43	theme	Zn2+	222:225	arg1	salts					201:205	salts	201:205	salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+	201:240	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	43	theme	Zn2+	222:225	arg1	component					259:267	the inorganic component	245:267	the inorganic component	245:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	4	44	theme	free	608:611	arg1	enzyme					613:618	free enzyme	608:618	free enzyme	608:618	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	4	45	theme	Ca2+	505:508	arg1	nanoflowers					525:535	Ca2+, Mn2+ and Co2+ nanoflowers	505:535	Ca2+, Mn2+ and Co2+ nanoflowers	505:535	Ca2+, Mn2+ and Co2+ nanoflowers showed a level of lactase activity equivalent to their same content of free enzyme.					
32061698	1	46	theme	hybrid	158:163	arg1	β-galactosidase					110:124	Thermostable β-galactosidase	97:124	Thermostable β-galactosidase (TmLac)	97:132	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	46	theme	hybrid	158:163	arg1	nanoflowers					183:193	hybrid inorganic-protein nanoflowers	158:193	hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component	158:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	47	theme	Ca2+	237:240	arg1	salts					201:205	salts	201:205	salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+	201:240	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
32061698	1	47	theme	Ca2+	237:240	arg1	component					259:267	the inorganic component	245:267	the inorganic component	245:267	Thermostable β-galactosidase (TmLac) has been immobilized as hybrid inorganic-protein nanoflowers using salts of Cu2+, Mn2+, Zn2+, Co2+ and Ca2+ as the inorganic component.					
33726643	2	0	from	women	393:397	arg1	common					368:373	common	368:373	common	368:373	This is a disease affected by many factors and is more common in postmenopausal women.					
33726643	6	1	theme	intestinal	942:951	arg1	flora					953:957	the intestinal flora	938:957	the intestinal flora	938:957	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	6	1	theme	intestinal	942:951	arg1	system					972:977	a complex system	962:977	a complex system that affects and regulates the absorption of calcium and vitamin D	962:1044	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	3	2	theme	bone	462:465	arg1	resorption					467:476	bone resorption	462:476	bone resorption	462:476	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	11	3	theme	intestinal	1588:1597	arg1	flora					1599:1603	the intestinal flora	1584:1603	the intestinal flora	1584:1603	Changes in the intestinal flora are closely related to osteopenia and/or osteoporosis.					
33726643	7	4	theme	own	1104:1106	arg1	secretion					1108:1116	its own secretion	1100:1116	its own secretion of related influencing factors and its own activities	1100:1170	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	0	5	from	Role	4:7	arg1	Relevance					70:78	Immune Relevance	63:78	Immune Relevance	63:78	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.					
33726643	0	5	from	Role	4:7	arg1	Osteolastogenesis					41:57	Osteolastogenesis	41:57	Osteolastogenesis	41:57	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.					
33726643	12	6	theme	bone	1738:1741	arg1	quality					1743:1749	bone quality	1738:1749	bone quality	1738:1749	Changing its composition and activity may be a reliable strategy for altering bone quality, although further clinical studies are needed to determine the correct human method.					
33726643	10	7	theme	probiotics	1494:1503	arg1	role					1486:1489	the role	1482:1489	the role of probiotics in different animals	1482:1524	However, many studies have shown that the role of probiotics in different animals varies with gender, age and hormone secretion.					
33726643	5	8	located	observed	786:793	arg2	It					776:777	It	776:777	It	776:777	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	5	8	located	observed	786:793	arg1	models					810:815	mouse-based models	798:815	mouse-based models	798:815	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	7	9	theme	intestine	1082:1090	arg1	permeability					1062:1073	the permeability	1058:1073	the permeability of the intestine	1058:1090	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	9	10	theme	data	1438:1441	arg1	scarcity					1417:1424	the scarcity	1413:1424	the scarcity of research data	1413:1441	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	5	11	theme	new	875:877	arg1	target					879:884	a potential new target	863:884	a potential new target for changing bone mineral density (BMD)	863:924	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	5	11	theme	new	875:877	arg1	flora					850:854	intestinal flora	839:854	intestinal flora	839:854	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	12	12	theme	correct	1814:1820	arg1	method					1828:1833	the correct human method	1810:1833	the correct human method	1810:1833	Changing its composition and activity may be a reliable strategy for altering bone quality, although further clinical studies are needed to determine the correct human method.					
33726643	12	13	theme	further	1761:1767	arg1	studies					1778:1784	further clinical studies	1761:1784	further clinical studies	1761:1784	Changing its composition and activity may be a reliable strategy for altering bone quality, although further clinical studies are needed to determine the correct human method.					
33726643	1	14	from	people	136:141	arg1	common					102:107	common	102:107	common	102:107	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	3	15	theme	fracture	501:508	arg1	risk					493:496	the risk	489:496	the risk of fracture	489:508	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	7	16	theme	own	1157:1159	arg1	activities					1161:1170	its own activities	1153:1170	its own activities	1153:1170	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	7	17	theme	activities	1161:1170	arg1	secretion					1108:1116	its own secretion	1100:1116	its own secretion of related influencing factors and its own activities	1100:1170	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	4	18	from	impact	685:690	arg1	metabolism					725:734	bone metabolism	720:734	bone metabolism in terms of immunity	720:755	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	4	19	theme	microflora	706:715	arg1	impact					685:690	a strong impact	676:690	a strong impact of intestinal microflora on bone metabolism in terms of immunity	676:755	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	5	20	theme	intestinal	839:848	arg1	target					879:884	a potential new target	863:884	a potential new target for changing bone mineral density (BMD)	863:924	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	5	20	theme	intestinal	839:848	arg1	flora					850:854	intestinal flora	839:854	intestinal flora	839:854	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	1	21	theme	main	152:155	arg1	feature					157:163	its main feature	148:163	its main feature	148:163	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	1	21	theme	main	152:155	arg1	disease					170:176	a disease	168:176	a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture	168:310	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	1	22	from	increase	279:286	arg1	risk					295:298	the risk	291:298	the risk of fracture	291:310	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	6	23	theme	vitamin	1036:1042	arg1	D					1044:1044	vitamin D	1036:1044	vitamin D	1036:1044	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	4	24	theme	intestinal	695:704	arg1	microflora					706:715	intestinal microflora	695:715	intestinal microflora	695:715	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	12	25	theme	reliable	1707:1714	arg1	strategy					1716:1723	a reliable strategy	1705:1723	a reliable strategy for altering bone quality	1705:1749	Changing its composition and activity may be a reliable strategy for altering bone quality, although further clinical studies are needed to determine the correct human method.					
33726643	6	26	theme	complex	964:970	arg1	flora					953:957	the intestinal flora	938:957	the intestinal flora	938:957	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	6	26	theme	complex	964:970	arg1	system					972:977	a complex system	962:977	a complex system that affects and regulates the absorption of calcium and vitamin D	962:1044	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	10	27	from	role	1486:1489	arg1	animals					1518:1524	different animals	1508:1524	different animals	1508:1524	However, many studies have shown that the role of probiotics in different animals varies with gender, age and hormone secretion.					
33726643	9	28	from	impact	1343:1348	arg1	humans					1382:1387	humans	1382:1387	humans	1382:1387	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	4	29	theme	recent	608:613	arg1	years					615:619	recent years	608:619	recent years	608:619	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	10	30	theme	different	1508:1516	arg1	animals					1518:1524	different animals	1508:1524	different animals	1508:1524	However, many studies have shown that the role of probiotics in different animals varies with gender, age and hormone secretion.					
33726643	4	31	theme	microorganisms	660:673	arg1	study					640:644	the in-depth study	627:644	the in-depth study of intestinal microorganisms	627:673	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	2	32	theme	many	343:346	arg1	factors					348:354	many factors	343:354	many factors	343:354	This is a disease affected by many factors and is more common in postmenopausal women.					
33726643	0	33	theme	Probiotics	12:21	arg1	Role					4:7	The Role	0:7	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.	0:79	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.					
33726643	7	34	theme	factors	1141:1147	arg1	secretion					1108:1116	its own secretion	1100:1116	its own secretion of related influencing factors and its own activities	1100:1170	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	4	35	theme	strong	678:683	arg1	impact					685:690	a strong impact	676:690	a strong impact of intestinal microflora on bone metabolism in terms of immunity	676:755	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	2	36	theme	postmenopausal	378:391	arg1	women					393:397	postmenopausal women	378:397	postmenopausal women	378:397	This is a disease affected by many factors and is more common in postmenopausal women.					
33726643	6	37	theme	D	1044:1044	arg1	absorption					1010:1019	the absorption	1006:1019	the absorption of calcium and vitamin D	1006:1044	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	1	38	theme	corresponding	265:277	arg1	increase					279:286	a corresponding increase	263:286	a corresponding increase in the risk of fracture	263:310	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	8	39	theme	RANKL	1309:1313	arg1	system					1315:1320	the RANKL system	1305:1320	the RANKL system	1305:1320	Besides that, T helper cells, lymphocytes, TNF, IL-17, and the RANKL system play a key role.					
33726643	5	40	theme	potential	865:873	arg1	target					879:884	a potential new target	863:884	a potential new target for changing bone mineral density (BMD)	863:924	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	5	40	theme	potential	865:873	arg1	flora					850:854	intestinal flora	839:854	intestinal flora	839:854	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	9	41	theme	prebiotics	1368:1377	arg1	controversial					1392:1404	controversial	1392:1404	controversial	1392:1404	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	9	41	theme	prebiotics	1368:1377	arg1	impact					1343:1348	The impact	1339:1348	The impact of probiotics and prebiotics on humans	1339:1387	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	3	42	theme	osteoporosis	428:439	arg1	treatment					415:423	the treatment	411:423	the treatment of osteoporosis	411:439	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	0	43	theme	Prebiotics	27:36	arg1	Role					4:7	The Role	0:7	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.	0:79	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.					
33726643	4	44	theme	in-depth	631:638	arg1	study					640:644	the in-depth study	627:644	the in-depth study of intestinal microorganisms	627:673	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	3	45	theme	good	591:594	arg1	results					596:602	good results	591:602	good results	591:602	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	7	46	theme	human	1210:1214	arg1	hormones					1216:1223	human hormones	1210:1223	human hormones	1210:1223	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	10	47	theme	many	1453:1456	arg1	studies					1458:1464	many studies	1453:1464	many studies	1453:1464	However, many studies have shown that the role of probiotics in different animals varies with gender, age and hormone secretion.					
33726643	3	48	theme	osteoporosis	565:576	arg1	treatment					552:560	the treatment	548:560	the treatment of osteoporosis	548:576	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	8	49	theme	helper	1262:1267	arg1	cells					1269:1273	T helper cells	1260:1273	T helper cells	1260:1273	Besides that, T helper cells, lymphocytes, TNF, IL-17, and the RANKL system play a key role.					
33726643	1	50	theme	bone	195:198	arg1	reduction					205:213	bone mass reduction	195:213	bone mass reduction	195:213	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	12	51	theme	human	1822:1826	arg1	method					1828:1833	the correct human method	1810:1833	the correct human method	1810:1833	Changing its composition and activity may be a reliable strategy for altering bone quality, although further clinical studies are needed to determine the correct human method.					
33726643	1	52	dep	reduction	205:213	arg1	changes					239:245	changes	239:245	changes	239:245	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	3	53	theme	drugs	524:528	arg1	drugs					524:528	drugs	524:528	drugs	524:528	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	3	53	theme	drugs	524:528	arg1	variety					513:519	a variety	511:519	a variety of drugs	511:528	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	6	54	theme	calcium	1024:1030	arg1	absorption					1010:1019	the absorption	1006:1019	the absorption of calcium and vitamin D	1006:1044	Therefore, the intestinal flora is a complex system that affects and regulates the absorption of calcium and vitamin D.					
33726643	11	55	from	Changes	1573:1579	arg1	flora					1599:1603	the intestinal flora	1584:1603	the intestinal flora	1584:1603	Changes in the intestinal flora are closely related to osteopenia and/or osteoporosis.					
33726643	7	56	theme	influencing	1129:1139	arg1	factors					1141:1147	related influencing factors	1121:1147	related influencing factors	1121:1147	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	4	57	theme	immunity	748:755	arg1	terms					739:743	terms	739:743	terms of immunity	739:755	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	1	58	theme	middle-aged	112:122	arg1	people					136:141	middle-aged and elderly people	112:141	middle-aged and elderly people	112:141	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	5	59	theme	bone	899:902	arg1	density					912:918	bone mineral density	899:918	bone mineral density (BMD)	899:924	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	5	59	theme	bone	899:902	arg1	BMD					921:923	BMD	921:923	BMD	921:923	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	4	60	theme	bone	720:723	arg1	metabolism					725:734	bone metabolism	720:734	bone metabolism in terms of immunity	720:755	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	9	61	theme	research	1429:1436	arg1	data					1438:1441	research data	1429:1441	research data	1429:1441	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	3	62	used	used	540:543	arg2	drugs					524:528	drugs	524:528	drugs	524:528	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	3	62	used	used	540:543	arg2	variety					513:519	a variety	511:519	a variety of drugs	511:528	Currently, the treatment of osteoporosis is mainly to prevent bone resorption and reduce the risk of fracture; a variety of drugs have been used in the treatment of osteoporosis and achieved good results.					
33726643	1	63	from	common	102:107	arg1	people					136:141	middle-aged and elderly people	112:141	middle-aged and elderly people	112:141	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	1	64	theme	mass	200:203	arg1	reduction					205:213	bone mass reduction	195:213	bone mass reduction	195:213	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	10	65	theme	hormone	1554:1560	arg1	secretion					1562:1570	hormone secretion	1554:1570	hormone secretion	1554:1570	However, many studies have shown that the role of probiotics in different animals varies with gender, age and hormone secretion.					
33726643	8	66	theme	key	1329:1331	arg1	role					1333:1336	a key role	1327:1336	a key role	1327:1336	Besides that, T helper cells, lymphocytes, TNF, IL-17, and the RANKL system play a key role.					
33726643	5	67	theme	mineral	904:910	arg1	density					912:918	bone mineral density	899:918	bone mineral density (BMD)	899:924	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	5	67	theme	mineral	904:910	arg1	BMD					921:923	BMD	921:923	BMD	921:923	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	2	68	from	common	368:373	arg1	women					393:397	postmenopausal women	378:397	postmenopausal women	378:397	This is a disease affected by many factors and is more common in postmenopausal women.					
33726643	1	69	theme	elderly	128:134	arg1	people					136:141	middle-aged and elderly people	112:141	middle-aged and elderly people	112:141	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	4	70	theme	intestinal	649:658	arg1	microorganisms					660:673	intestinal microorganisms	649:673	intestinal microorganisms	649:673	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	0	71	theme	Immune	63:68	arg1	Relevance					70:78	Immune Relevance	63:78	Immune Relevance	63:78	The Role of Probiotics and Prebiotics in Osteolastogenesis and Immune Relevance.					
33726643	8	72	theme	T	1260:1260	arg1	cells					1269:1273	T helper cells	1260:1273	T helper cells	1260:1273	Besides that, T helper cells, lymphocytes, TNF, IL-17, and the RANKL system play a key role.					
33726643	4	73	from	metabolism	725:734	arg1	terms					739:743	terms	739:743	terms of immunity	739:755	In recent years, with the in-depth study of intestinal microorganisms, a strong impact of intestinal microflora on bone metabolism in terms of immunity has been reported.					
33726643	5	74	theme	mouse-based	798:808	arg1	models					810:815	mouse-based models	798:815	mouse-based models	798:815	It can be observed in mouse-based models, which indicates that intestinal flora may be a potential new target for changing bone mineral density (BMD).					
33726643	7	75	theme	hormones	1216:1223	arg1	response					1236:1243	immune response	1229:1243	immune response	1229:1243	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	7	75	theme	hormones	1216:1223	arg1	secretion					1197:1205	the secretion	1193:1205	the secretion of human hormones	1193:1223	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	1	76	theme	fracture	303:310	arg1	risk					295:298	the risk	291:298	the risk of fracture	291:310	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
33726643	7	77	theme	immune	1229:1234	arg1	response					1236:1243	immune response	1229:1243	immune response	1229:1243	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	12	78	theme	clinical	1769:1776	arg1	studies					1778:1784	further clinical studies	1761:1784	further clinical studies	1761:1784	Changing its composition and activity may be a reliable strategy for altering bone quality, although further clinical studies are needed to determine the correct human method.					
33726643	9	79	theme	probiotics	1353:1362	arg1	controversial					1392:1404	controversial	1392:1404	controversial	1392:1404	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	9	79	theme	probiotics	1353:1362	arg1	impact					1343:1348	The impact	1339:1348	The impact of probiotics and prebiotics on humans	1339:1387	The impact of probiotics and prebiotics on humans is controversial due to the scarcity of research data.					
33726643	7	80	theme	related	1121:1127	arg1	factors					1141:1147	related influencing factors	1121:1147	related influencing factors	1121:1147	It affects the permeability of the intestine through its own secretion of related influencing factors and its own activities, and further affects the secretion of human hormones and immune response.					
33726643	1	81	theme	bone	219:222	arg1	microstructure					224:237	bone microstructure	219:237	bone microstructure	219:237	Osteoporosis is very common in middle-aged and elderly people, and its main feature is a disease characterized by bone mass reduction and bone microstructure changes, accompanied by a corresponding increase in the risk of fracture.					
32045817	0	0	theme	oil	62:64	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.	0:187	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	4	1	theme	chemical	530:537	arg1	composition					539:549	The chemical composition	526:549	The chemical composition of the encapsulated EO	526:572	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	5	2	theme	good	688:691	arg1	protection					698:707	good core protection	688:707	good core protection	688:707	The GC and NMR analyses indicated good core protection with the materials used.					
32045817	0	3	theme	essential	52:60	arg1	L.					48:49	Piper nigrum L.	35:49	Piper nigrum L.	35:49	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	3	theme	essential	52:60	arg1	oil					62:64	the black pepper (Piper nigrum L.) essential oil	17:64	the black pepper (Piper nigrum L.) essential oil	17:64	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	5	4	theme	NMR	665:667	arg1	analyses					669:676	The GC and NMR analyses	654:676	The GC and NMR analyses	654:676	The GC and NMR analyses indicated good core protection with the materials used.					
32045817	0	5	dep	coacervates	105:115	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	5	dep	coacervates	105:115	arg1	characterization					129:144	Structural characterization	118:144	Structural characterization	118:144	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	5	dep	coacervates	105:115	arg1	conditions					177:186	simulated gastrointestinal conditions	150:186	simulated gastrointestinal conditions	150:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	6	theme	lactoferrin-sodium	69:86	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	6	theme	lactoferrin-sodium	69:86	arg1	characterization					129:144	Structural characterization	118:144	Structural characterization	118:144	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	6	theme	lactoferrin-sodium	69:86	arg1	conditions					177:186	simulated gastrointestinal conditions	150:186	simulated gastrointestinal conditions	150:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	7	7	theme	gastric	882:888	arg1	digestion					890:898	gastric digestion	882:898	gastric digestion	882:898	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	4	8	theme	nuclear	620:626	arg1	resonance					637:645	nuclear magnetic resonance	620:645	nuclear magnetic resonance (NMR)	620:651	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	4	8	theme	nuclear	620:626	arg1	NMR					648:650	NMR	648:650	NMR	648:650	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	6	9	dep	in	786:787	arg1	vitro					789:793	vitro	789:793	vitro	789:793	The stability of the black pepper EO capsules under in vitro digestion was evaluated.					
32045817	0	10	theme	complex	97:103	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	10	theme	complex	97:103	arg1	characterization					129:144	Structural characterization	118:144	Structural characterization	118:144	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	10	theme	complex	97:103	arg1	conditions					177:186	simulated gastrointestinal conditions	150:186	simulated gastrointestinal conditions	150:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	8	11	used	used	993:996	arg2	capsules					977:984	these capsules	971:984	these capsules	971:984	These results suggest that these capsules can be used to transport active ingredients and that they are resistant to oral and gastric conditions that were tested in vitro.					
32045817	8	12	theme	oral	1061:1064	arg1	conditions					1078:1087	oral and gastric conditions	1061:1087	oral and gastric conditions that were tested in vitro	1061:1113	These results suggest that these capsules can be used to transport active ingredients and that they are resistant to oral and gastric conditions that were tested in vitro.					
32045817	7	13	from	digestion	890:898	arg1	digestion					933:941	intestinal digestion	922:941	intestinal digestion	922:941	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	7	14	theme	EO	872:873	arg1	release					857:863	the low release	849:863	the low release of the EO	849:873	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	0	15	theme	alginate	88:95	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	15	theme	alginate	88:95	arg1	characterization					129:144	Structural characterization	118:144	Structural characterization	118:144	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	15	theme	alginate	88:95	arg1	conditions					177:186	simulated gastrointestinal conditions	150:186	simulated gastrointestinal conditions	150:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	8	16	theme	active	1011:1016	arg1	ingredients					1018:1028	active ingredients	1011:1028	active ingredients	1011:1028	These results suggest that these capsules can be used to transport active ingredients and that they are resistant to oral and gastric conditions that were tested in vitro.					
32045817	1	17	theme	Black	189:193	arg1	EO					217:218	EO	217:218	EO	217:218	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	1	17	theme	Black	189:193	arg1	oil					212:214	Black pepper essential oil	189:214	Black pepper essential oil (EO)	189:219	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	8	18	theme	gastric	1070:1076	arg1	conditions					1078:1087	oral and gastric conditions	1061:1087	oral and gastric conditions that were tested in vitro	1061:1113	These results suggest that these capsules can be used to transport active ingredients and that they are resistant to oral and gastric conditions that were tested in vitro.					
32045817	4	19	theme	gas	592:594	arg1	GC					612:613	GC	612:613	GC	612:613	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	4	19	theme	gas	592:594	arg1	chromatography					596:609	gas chromatography	592:609	gas chromatography (GC)	592:614	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	3	20	theme	morphological	419:431	arg1	characteristics					433:447	Chemical and morphological characteristics	406:447	Chemical and morphological characteristics	406:447	Chemical and morphological characteristics suggest that the EO was encapsulated in a lactoferrin/sodium alginate shell.					
32045817	1	21	theme	pepper	195:200	arg1	EO					217:218	EO	217:218	EO	217:218	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	1	21	theme	pepper	195:200	arg1	oil					212:214	Black pepper essential oil	189:214	Black pepper essential oil (EO)	189:219	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	3	22	theme	Chemical	406:413	arg1	characteristics					433:447	Chemical and morphological characteristics	406:447	Chemical and morphological characteristics	406:447	Chemical and morphological characteristics suggest that the EO was encapsulated in a lactoferrin/sodium alginate shell.					
32045817	1	23	theme	sodium	283:288	arg1	alginate					290:297	sodium alginate	283:297	sodium alginate	283:297	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	5	24	theme	GC	658:659	arg1	analyses					669:676	The GC and NMR analyses	654:676	The GC and NMR analyses	654:676	The GC and NMR analyses indicated good core protection with the materials used.					
32045817	6	25	theme	in	786:787	arg1	digestion					795:803	in vitro digestion	786:803	in vitro digestion	786:803	The stability of the black pepper EO capsules under in vitro digestion was evaluated.					
32045817	7	26	from	release	911:917	arg1	digestion					933:941	intestinal digestion	922:941	intestinal digestion	922:941	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	1	27	theme	essential	202:210	arg1	EO					217:218	EO	217:218	EO	217:218	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	1	27	theme	essential	202:210	arg1	oil					212:214	Black pepper essential oil	189:214	Black pepper essential oil (EO)	189:219	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	0	28	theme	black	21:25	arg1	L.					48:49	Piper nigrum L.	35:49	Piper nigrum L.	35:49	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	28	theme	black	21:25	arg1	oil					62:64	the black pepper (Piper nigrum L.) essential oil	17:64	the black pepper (Piper nigrum L.) essential oil	17:64	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	7	29	theme	low	853:855	arg1	release					857:863	the low release	849:863	the low release of the EO	849:873	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	6	30	theme	pepper	761:766	arg1	capsules					771:778	the black pepper EO capsules	751:778	the black pepper EO capsules	751:778	The stability of the black pepper EO capsules under in vitro digestion was evaluated.					
32045817	3	31	theme	lactoferrin/sodium	491:508	arg1	shell					519:523	a lactoferrin/sodium alginate shell	489:523	a lactoferrin/sodium alginate shell	489:523	Chemical and morphological characteristics suggest that the EO was encapsulated in a lactoferrin/sodium alginate shell.					
32045817	0	32	theme	pepper	27:32	arg1	L.					48:49	Piper nigrum L.	35:49	Piper nigrum L.	35:49	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	32	theme	pepper	27:32	arg1	oil					62:64	the black pepper (Piper nigrum L.) essential oil	17:64	the black pepper (Piper nigrum L.) essential oil	17:64	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	4	33	theme	EO	571:572	arg1	composition					539:549	The chemical composition	526:549	The chemical composition of the encapsulated EO	526:572	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	0	34	theme	Structural	118:127	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	34	theme	Structural	118:127	arg1	characterization					129:144	Structural characterization	118:144	Structural characterization	118:144	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	6	35	theme	capsules	771:778	arg1	stability					738:746	The stability	734:746	The stability of the black pepper EO capsules under in vitro digestion	734:803	The stability of the black pepper EO capsules under in vitro digestion was evaluated.					
32045817	0	36	theme	nigrum	41:46	arg1	L.					48:49	Piper nigrum L.	35:49	Piper nigrum L.	35:49	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	36	theme	nigrum	41:46	arg1	oil					62:64	the black pepper (Piper nigrum L.) essential oil	17:64	the black pepper (Piper nigrum L.) essential oil	17:64	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	4	37	theme	encapsulated	558:569	arg1	EO					571:572	the encapsulated EO	554:572	the encapsulated EO	554:572	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	0	38	theme	simulated	150:158	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	38	theme	simulated	150:158	arg1	conditions					177:186	simulated gastrointestinal conditions	150:186	simulated gastrointestinal conditions	150:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	6	39	theme	EO	768:769	arg1	capsules					771:778	the black pepper EO capsules	751:778	the black pepper EO capsules	751:778	The stability of the black pepper EO capsules under in vitro digestion was evaluated.					
32045817	0	40	theme	Piper	35:39	arg1	L.					48:49	Piper nigrum L.	35:49	Piper nigrum L.	35:49	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	40	theme	Piper	35:39	arg1	oil					62:64	the black pepper (Piper nigrum L.) essential oil	17:64	the black pepper (Piper nigrum L.) essential oil	17:64	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	1	41	theme	cross-linking	327:339	arg1	transglutaminase					305:320	transglutaminase	305:320	transglutaminase	305:320	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	1	41	theme	cross-linking	327:339	arg1	agent					341:345	a cross-linking agent	325:345	a cross-linking agent	325:345	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	5	42	theme	core	693:696	arg1	protection					698:707	good core protection	688:707	good core protection	688:707	The GC and NMR analyses indicated good core protection with the materials used.					
32045817	1	43	with	coacervation	249:260	arg1	lactoferrin					267:277	lactoferrin	267:277	lactoferrin	267:277	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	1	43	with	coacervation	249:260	arg1	alginate					290:297	sodium alginate	283:297	sodium alginate	283:297	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
32045817	2	44	theme	encapsulation	352:364	arg1	efficiency					366:375	The encapsulation efficiency	348:375	The encapsulation efficiency	348:375	The encapsulation efficiency varied from 31.66 to 84.48%.					
32045817	6	45	theme	black	755:759	arg1	capsules					771:778	the black pepper EO capsules	751:778	the black pepper EO capsules	751:778	The stability of the black pepper EO capsules under in vitro digestion was evaluated.					
32045817	3	46	theme	alginate	510:517	arg1	shell					519:523	a lactoferrin/sodium alginate shell	489:523	a lactoferrin/sodium alginate shell	489:523	Chemical and morphological characteristics suggest that the EO was encapsulated in a lactoferrin/sodium alginate shell.					
32045817	7	47	theme	intestinal	922:931	arg1	digestion					933:941	intestinal digestion	922:941	intestinal digestion	922:941	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	0	48	theme	gastrointestinal	160:175	arg1	coacervates					105:115	lactoferrin-sodium alginate complex coacervates	69:115	lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions	69:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	0	48	theme	gastrointestinal	160:175	arg1	conditions					177:186	simulated gastrointestinal conditions	150:186	simulated gastrointestinal conditions	150:186	Encapsulation of the black pepper (Piper nigrum L.) essential oil by lactoferrin-sodium alginate complex coacervates: Structural characterization and simulated gastrointestinal conditions.					
32045817	2	49	dep	84.48	398:402	arg1	to					395:396	to	395:396	to	395:396	The encapsulation efficiency varied from 31.66 to 84.48%.					
32045817	7	50	theme	higher	904:909	arg1	release					911:917	higher release	904:917	higher release	904:917	Theses capsules demonstrated the low release of the EO during gastric digestion and higher release in intestinal digestion.					
32045817	4	51	theme	magnetic	628:635	arg1	resonance					637:645	nuclear magnetic resonance	620:645	nuclear magnetic resonance (NMR)	620:651	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	4	51	theme	magnetic	628:635	arg1	NMR					648:650	NMR	648:650	NMR	648:650	The chemical composition of the encapsulated EO was identified by gas chromatography (GC) and nuclear magnetic resonance (NMR).					
32045817	1	52	theme	complex	241:247	arg1	coacervation					249:260	complex coacervation	241:260	complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent	241:345	Black pepper essential oil (EO) was encapsulated by complex coacervation with lactoferrin and sodium alginate using transglutaminase as a cross-linking agent.					
34988116	4	0	theme	ions	1137:1140	arg1	intensities					1107:1117	summed intensities	1100:1117	summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	1100:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	6	1	theme	3-O-sulfated	1610:1621	arg1	acid					1633:1636	3-O-sulfated hexuronic acid	1610:1636	3-O-sulfated hexuronic acid	1610:1636	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	5	2	theme	sample	1313:1318	arg1	fractions					1320:1328	sample fractions	1313:1328	sample fractions enriched in negatively charged glycans	1313:1367	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	8	3	theme	adult	2289:2293	arg1	model					2334:2338	an alternative vertebrate model	2308:2338	an alternative vertebrate model	2308:2338	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	8	3	theme	adult	2289:2293	arg1	zebrafish					2295:2303	adult zebrafish	2289:2303	adult zebrafish	2289:2303	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	3	4	theme	major	789:793	arg1	glycotopes					795:804	all the major glycotopes	781:804	all the major glycotopes of interest	781:816	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	0	5	theme	Discovery	0:8	arg1	Sulfoglycomics					10:23	Discovery Sulfoglycomics	0:23	Discovery Sulfoglycomics	0:23	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	1	6	theme	glycotopes	224:233	arg1	mapping					204:210	Mass spectrometry-based high-sensitivity mapping	163:210	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes	163:233	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	8	7	from	those	2262:2266	arg1	distinct					2248:2255	distinct	2248:2255	distinct	2248:2255	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	4	8	theme	acid	1232:1235	arg1	variants					1237:1244	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants	899:1244	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants	899:1244	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	2	9	theme	Unambiguous	367:377	arg1	identification					379:392	Unambiguous identification	367:392	Unambiguous identification of adult zebrafish glycotopes	367:422	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	7	10	theme	underlying	2058:2067	arg1	sulfotransferases					2069:2085	the underlying sulfotransferases	2054:2085	the underlying sulfotransferases	2054:2085	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	2	11	theme	neuraminic	632:641	arg1	acids					643:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	8	12	theme	alternative	2311:2321	arg1	model					2334:2338	an alternative vertebrate model	2308:2338	an alternative vertebrate model	2308:2338	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	8	12	theme	alternative	2311:2321	arg1	zebrafish					2295:2303	adult zebrafish	2289:2303	adult zebrafish	2289:2303	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	4	13	theme	each	1198:1201	arg1	distribution					1159:1170	the distribution	1155:1170	the distribution	1155:1170	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	13	theme	each	1198:1201	arg1	abundance					1185:1193	relative abundance	1176:1193	relative abundance	1176:1193	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	0	14	theme	Brain-Specific	136:149	arg1	Glycotopes					151:160	Adult Zebrafish Brain-Specific Glycotopes	120:160	Adult Zebrafish Brain-Specific Glycotopes	120:160	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	4	15	theme	extracted	1020:1028	arg1	chromatograms					1034:1046	overlaid extracted ion chromatograms	1011:1046	overlaid extracted ion chromatograms of resolved glycan species	1011:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	3	16	theme	nano-LC-MS/MS	850:862	arg1	analysis					864:871	direct nano-LC-MS/MS analysis	843:871	direct nano-LC-MS/MS analysis of permethylated glycans	843:896	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	4	17	theme	MS2	1133:1135	arg1	ions					1137:1140	diagnostic MS2 ions	1122:1140	diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	1122:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	1	18	theme	MS3	267:269	arg1	ions					271:274	diagnostic MS2 and/or MS3 ions	245:274	ions	271:274	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	4	19	theme	glycomic	978:985	arg1	maps					997:1000	organ-specific glycomic reference maps	963:1000	organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species	963:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	20	from	maps	997:1000	arg1	intestine-					924:933	intestine-	924:933	intestine-	924:933	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	20	from	maps	997:1000	arg1	ovary-derived					940:952	ovary-derived	940:952	ovary-derived	940:952	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	20	from	maps	997:1000	arg1	brain-					916:921	the brain-	912:921	the brain-	912:921	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	3	21	theme	glycans	890:896	arg1	analysis					864:871	direct nano-LC-MS/MS analysis	843:871	direct nano-LC-MS/MS analysis of permethylated glycans	843:896	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	4	22	from	Homing	899:904	arg1	brain-					916:921	the brain-	912:921	the brain-	912:921	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	22	from	Homing	899:904	arg1	intestine-					924:933	intestine-	924:933	intestine-	924:933	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	22	from	Homing	899:904	arg1	maps					997:1000	organ-specific glycomic reference maps	963:1000	organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species	963:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	22	from	Homing	899:904	arg1	ovary-derived					940:952	ovary-derived	940:952	ovary-derived	940:952	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	22	from	Homing	899:904	arg1	samples					954:960	on the brain-, intestine-, and ovary-derived samples	909:960	on the brain-, intestine-, and ovary-derived samples	909:960	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	22	from	Homing	899:904	arg1	charts					1090:1095	composite charts	1080:1095	composite charts	1080:1095	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	7	23	theme	structural	1800:1809	arg1	features					1811:1818	Other characteristic structural features	1779:1818	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work	1779:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	4	24	theme	species	1067:1073	arg1	chromatograms					1034:1046	overlaid extracted ion chromatograms	1011:1046	overlaid extracted ion chromatograms of resolved glycan species	1011:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	2	25	theme	basic	507:511	arg1	block					522:526	the basic building block	503:526	the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	503:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	0	26	theme	Ions	75:78	arg1	Identification					29:42	Identification	29:42	Identification	29:42	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	0	26	theme	Ions	75:78	arg1	Sulfoglycomics					10:23	Discovery Sulfoglycomics	0:23	Discovery Sulfoglycomics	0:23	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	3	27	theme	various	703:709	arg1	N-					726:727	various organ-specific N-	703:727	various organ-specific N-	703:727	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	1	28	theme	high-sensitivity	187:202	arg1	mapping					204:210	Mass spectrometry-based high-sensitivity mapping	163:210	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes	163:233	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	7	29	theme	Other	1779:1783	arg1	features					1811:1818	Other characteristic structural features	1779:1818	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work	1779:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	30	theme	zebrafish-specific	2154:2171	arg1	glycotopes					2173:2182	zebrafish-specific glycotopes	2154:2182	zebrafish-specific glycotopes	2154:2182	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	0	31	theme	High-Sensitivity	84:99	arg1	Mapping					109:115	High-Sensitivity Precise Mapping	84:115	High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes	84:160	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	4	32	from	ovary-derived	940:952	arg1	acid					1232:1235	sialic acid	1225:1235	sialic acid	1225:1235	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	32	from	ovary-derived	940:952	arg1	Homing					899:904	Homing	899:904	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	899:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	2	33	theme	N-glycolyl	606:615	arg1	acids					643:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	2	34	from	block	522:526	arg1	presence					463:470	the presence	459:470	the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	459:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	2	35	theme	N-acetyl	596:603	arg1	acids					643:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	5	36	theme	full	1406:1409	arg1	range					1411:1415	a full range	1404:1415	a full range of sulfated glycotopes	1404:1438	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	0	37	theme	Adult	120:124	arg1	Glycotopes					151:160	Adult Zebrafish Brain-Specific Glycotopes	120:160	Adult Zebrafish Brain-Specific Glycotopes	120:160	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	6	38	theme	Gal	1514:1516	arg1	3-O-sulfation					1488:1500	3-O-sulfation	1488:1500	3-O-sulfation of terminal Gal	1488:1516	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	2	39	gly	sialylated	582:591	arg1	Galβ1-4GlcNAc					531:543	Galβ1-4GlcNAc	531:543	Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	531:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	2	40	theme	building	513:520	arg1	block					522:526	the basic building block	503:526	the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	503:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	1	41	theme	substituents	334:345	arg1	location					322:329	the location	318:329	the location of substituents including sulfates	318:364	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	8	42	theme	glyco-neurobiology	2378:2395	arg1	studies					2397:2403	brain-related glyco-neurobiology studies	2364:2403	brain-related glyco-neurobiology studies	2364:2403	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	8	43	theme	particular	2188:2197	arg1	importance					2199:2208	particular importance	2188:2208	particular importance	2188:2208	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	3	44	theme	adult	746:750	arg1	zebrafish					752:760	adult zebrafish	746:760	adult zebrafish	746:760	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	2	45	theme	β4-galactosylation	481:498	arg1	presence					463:470	the presence	459:470	the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	459:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	6	46	located	found	1531:1535	arg2	3-O-sulfation					1488:1500	3-O-sulfation	1488:1500	3-O-sulfation of terminal Gal	1488:1516	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	6	46	located	found	1531:1535	arg1	particular					1476:1485	particular	1476:1485	particular	1476:1485	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	4	47	theme	summed	1100:1105	arg1	intensities					1107:1117	summed intensities	1100:1117	summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	1100:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	5	48	theme	adult	1456:1460	arg1	zebrafish					1462:1470	adult zebrafish	1456:1470	adult zebrafish	1456:1470	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	2	49	theme	glycotopes	413:422	arg1	identification					379:392	Unambiguous identification	367:392	Unambiguous identification of adult zebrafish glycotopes	367:422	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	0	50	theme	Characteristic	51:64	arg1	Ions					75:78	the Characteristic Fragment Ions	47:78	the Characteristic Fragment Ions	47:78	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	4	51	from	charts	1090:1095	arg1	intestine-					924:933	intestine-	924:933	intestine-	924:933	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	51	from	charts	1090:1095	arg1	ovary-derived					940:952	ovary-derived	940:952	ovary-derived	940:952	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	51	from	charts	1090:1095	arg1	brain-					916:921	the brain-	912:921	the brain-	912:921	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	7	52	theme	zebrafish	1963:1971	arg1	atlas					1981:1985	our adult zebrafish glycome atlas	1953:1985	our adult zebrafish glycome atlas	1953:1985	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	6	53	from	HexNAc	1564:1569	arg1	epitope					1650:1656	HNK-1 epitope	1644:1656	HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc)	1644:1685	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	6	53	from	HexNAc	1564:1569	arg1	LacdiNAc					1595:1602	LacdiNAc	1595:1602	LacdiNAc	1595:1602	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	6	53	from	HexNAc	1564:1569	arg1	GalNAcβ1-4GlcNAc					1577:1592	GalNAcβ1-4GlcNAc	1577:1592	GalNAcβ1-4GlcNAc (LacdiNAc)	1577:1603	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	6	53	from	HexNAc	1564:1569	arg1	SO3-3GlcAβ1-3Galβ1-4GlcNAc					1659:1684	SO3-3GlcAβ1-3Galβ1-4GlcNAc	1659:1684	SO3-3GlcAβ1-3Galβ1-4GlcNAc	1659:1684	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	6	53	from	HexNAc	1564:1569	arg1	acid					1633:1636	3-O-sulfated hexuronic acid	1610:1636	3-O-sulfated hexuronic acid	1610:1636	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	7	54	theme	mode	1909:1912	arg1	work					1928:1931	this discovery mode sulfoglycomic work	1894:1931	this discovery mode sulfoglycomic work	1894:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	4	55	theme	relative	1176:1183	arg1	abundance					1185:1193	relative abundance	1176:1193	relative abundance	1176:1193	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	2	56	theme	adult	397:401	arg1	glycotopes					413:422	adult zebrafish glycotopes	397:422	adult zebrafish glycotopes	397:422	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	5	57	theme	fractions	1320:1328	arg1	analysis					1301:1308	negative mode analysis	1287:1308	negative mode analysis of sample fractions enriched in negatively charged glycans	1287:1367	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	2	58	from	β4-galactosylation	481:498	arg1	block					522:526	the basic building block	503:526	the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	503:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	8	59	theme	glycomic	2230:2237	arg1	features					2239:2246	glycomic features	2230:2246	glycomic features distinct from those of humans	2230:2276	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	5	60	theme	glycotopes	1429:1438	arg1	range					1411:1415	a full range	1404:1415	a full range of sulfated glycotopes	1404:1438	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	4	61	from	acid	1232:1235	arg1	brain-					916:921	the brain-	912:921	the brain-	912:921	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	61	from	acid	1232:1235	arg1	intestine-					924:933	intestine-	924:933	intestine-	924:933	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	61	from	acid	1232:1235	arg1	maps					997:1000	organ-specific glycomic reference maps	963:1000	organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species	963:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	61	from	acid	1232:1235	arg1	ovary-derived					940:952	ovary-derived	940:952	ovary-derived	940:952	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	61	from	acid	1232:1235	arg1	samples					954:960	on the brain-, intestine-, and ovary-derived samples	909:960	on the brain-, intestine-, and ovary-derived samples	909:960	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	61	from	acid	1232:1235	arg1	charts					1090:1095	composite charts	1080:1095	composite charts	1080:1095	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	8	62	theme	distinct	2248:2255	arg1	features					2239:2246	glycomic features	2230:2246	glycomic features distinct from those of humans	2230:2276	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	5	63	theme	mode	1296:1299	arg1	analysis					1301:1308	negative mode analysis	1287:1308	negative mode analysis of sample fractions enriched in negatively charged glycans	1287:1367	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	7	64	theme	functional	2130:2139	arg1	relevance					2141:2149	the functional relevance	2126:2149	the functional relevance of zebrafish-specific glycotopes	2126:2182	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	65	theme	diagnostic	1866:1875	arg1	ions					1877:1880	their diagnostic ions	1860:1880	their diagnostic ions detected in this discovery mode sulfoglycomic work	1860:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	4	66	theme	sialic	1225:1230	arg1	acid					1232:1235	sialic acid	1225:1235	sialic acid	1225:1235	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	3	67	theme	interest	809:816	arg1	glycotopes					795:804	all the major glycotopes	781:804	all the major glycotopes of interest	781:816	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	1	68	theme	terminal	215:222	arg1	glycotopes					224:233	terminal glycotopes	215:233	terminal glycotopes	215:233	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	7	69	theme	N-glycans	1839:1847	arg1	features					1811:1818	Other characteristic structural features	1779:1818	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work	1779:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	70	theme	glycosyltransferases	2091:2110	arg1	probing					2043:2049	probing	2043:2049	probing of the underlying sulfotransferases and glycosyltransferases	2043:2110	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	70	theme	glycosyltransferases	2091:2110	arg1	navigation					2028:2037	a more complete navigation	2012:2037	a more complete navigation	2012:2037	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	71	theme	O-	1832:1833	arg1	features					1811:1818	Other characteristic structural features	1779:1818	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work	1779:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	0	72	theme	Glycotopes	151:160	arg1	Mapping					109:115	High-Sensitivity Precise Mapping	84:115	High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes	84:160	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	1	73	theme	diagnostic	245:254	arg1	MS2					256:258	diagnostic MS2 and/or MS3 ions	245:274	MS2	256:258	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	7	74	theme	sulfotransferases	2069:2085	arg1	probing					2043:2049	probing	2043:2049	probing of the underlying sulfotransferases and glycosyltransferases	2043:2110	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	74	theme	sulfotransferases	2069:2085	arg1	navigation					2028:2037	a more complete navigation	2012:2037	a more complete navigation	2012:2037	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	6	75	dep	intestine	1736:1744	arg1	the					1732:1734	the	1732:1734	the	1732:1734	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	2	76	theme	deaminated	621:630	arg1	acids					643:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	N-acetyl, N-glycolyl, or deaminated neuraminic acids	596:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	4	77	theme	overlaid	1011:1018	arg1	chromatograms					1034:1046	overlaid extracted ion chromatograms	1011:1046	overlaid extracted ion chromatograms of resolved glycan species	1011:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	8	78	theme	vertebrate	2323:2332	arg1	model					2334:2338	an alternative vertebrate model	2308:2338	an alternative vertebrate model	2308:2338	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	8	78	theme	vertebrate	2323:2332	arg1	zebrafish					2295:2303	adult zebrafish	2289:2303	adult zebrafish	2289:2303	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	7	79	theme	sulfoglycomic	1914:1926	arg1	work					1928:1931	this discovery mode sulfoglycomic work	1894:1931	this discovery mode sulfoglycomic work	1894:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	3	80	theme	previous	662:669	arg1	groundwork					671:680	previous groundwork	662:680	previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish	662:760	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	4	81	theme	organ-specific	963:976	arg1	maps					997:1000	organ-specific glycomic reference maps	963:1000	organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species	963:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	3	82	theme	direct	843:848	arg1	analysis					864:871	direct nano-LC-MS/MS analysis	843:871	direct nano-LC-MS/MS analysis of permethylated glycans	843:896	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	3	83	theme	organ-specific	711:724	arg1	N-					726:727	various organ-specific N-	703:727	various organ-specific N-	703:727	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	6	84	theme	HNK-1	1644:1648	arg1	epitope					1650:1656	HNK-1 epitope	1644:1656	HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc)	1644:1685	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	6	84	theme	HNK-1	1644:1648	arg1	SO3-3GlcAβ1-3Galβ1-4GlcNAc					1659:1684	SO3-3GlcAβ1-3Galβ1-4GlcNAc	1659:1684	SO3-3GlcAβ1-3Galβ1-4GlcNAc	1659:1684	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	7	85	theme	sulfated	1823:1830	arg1	O-					1832:1833	sulfated O-	1823:1833	sulfated O-	1823:1833	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	4	86	theme	reference	987:995	arg1	maps					997:1000	organ-specific glycomic reference maps	963:1000	organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species	963:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	1	87	theme	spectrometry-based	168:185	arg1	mapping					204:210	Mass spectrometry-based high-sensitivity mapping	163:210	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes	163:233	Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differentiate linkage and define the location of substituents including sulfates.					
34988116	4	88	theme	glycan	1060:1065	arg1	species					1067:1073	resolved glycan species	1051:1073	resolved glycan species	1051:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	5	89	theme	charged	1353:1359	arg1	glycans					1361:1367	negatively charged glycans	1342:1367	negatively charged glycans	1342:1367	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	3	90	theme	permethylated	876:888	arg1	glycans					890:896	permethylated glycans	876:896	permethylated glycans	876:896	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	0	91	theme	Precise	101:107	arg1	Mapping					109:115	High-Sensitivity Precise Mapping	84:115	High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes	84:160	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	4	92	from	samples	954:960	arg1	intestine-					924:933	intestine-	924:933	intestine-	924:933	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	92	from	samples	954:960	arg1	ovary-derived					940:952	ovary-derived	940:952	ovary-derived	940:952	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	92	from	samples	954:960	arg1	brain-					916:921	the brain-	912:921	the brain-	912:921	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	7	93	theme	complete	2019:2026	arg1	navigation					2028:2037	a more complete navigation	2012:2037	a more complete navigation	2012:2037	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	6	94	theme	hexuronic	1623:1631	arg1	acid					1633:1636	3-O-sulfated hexuronic acid	1610:1636	3-O-sulfated hexuronic acid	1610:1636	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	7	95	theme	characteristic	1785:1798	arg1	features					1811:1818	Other characteristic structural features	1779:1818	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work	1779:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	96	located	detected	1882:1889	arg2	ions					1877:1880	their diagnostic ions	1860:1880	their diagnostic ions detected in this discovery mode sulfoglycomic work	1860:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	96	located	detected	1882:1889	arg1	work					1928:1931	this discovery mode sulfoglycomic work	1894:1931	this discovery mode sulfoglycomic work	1894:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	97	theme	glycotopes	2173:2182	arg1	relevance					2141:2149	the functional relevance	2126:2149	the functional relevance of zebrafish-specific glycotopes	2126:2182	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	4	98	theme	ion	1030:1032	arg1	chromatograms					1034:1046	overlaid extracted ion chromatograms	1011:1046	overlaid extracted ion chromatograms of resolved glycan species	1011:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	0	99	theme	Zebrafish	126:134	arg1	Glycotopes					151:160	Adult Zebrafish Brain-Specific Glycotopes	120:160	Adult Zebrafish Brain-Specific Glycotopes	120:160	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	4	100	from	intestine-	924:933	arg1	acid					1232:1235	sialic acid	1225:1235	sialic acid	1225:1235	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	100	from	intestine-	924:933	arg1	Homing					899:904	Homing	899:904	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	899:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	101	theme	resolved	1051:1058	arg1	species					1067:1073	resolved glycan species	1051:1073	resolved glycan species	1051:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	102	from	brain-	916:921	arg1	acid					1232:1235	sialic acid	1225:1235	sialic acid	1225:1235	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	102	from	brain-	916:921	arg1	Homing					899:904	Homing	899:904	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	899:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	103	theme	intensities	1107:1117	arg1	maps					997:1000	organ-specific glycomic reference maps	963:1000	organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species	963:1073	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	103	theme	intensities	1107:1117	arg1	samples					954:960	on the brain-, intestine-, and ovary-derived samples	909:960	on the brain-, intestine-, and ovary-derived samples	909:960	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	103	theme	intensities	1107:1117	arg1	charts					1090:1095	composite charts	1080:1095	composite charts	1080:1095	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	5	104	theme	first	1387:1391	arg1	time					1393:1396	the first time	1383:1396	the first time	1383:1396	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	6	105	theme	terminal	1505:1512	arg1	Gal					1514:1516	terminal Gal	1505:1516	terminal Gal	1505:1516	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	8	106	theme	importance	2199:2208	arg1	knowledge					2217:2225	the knowledge	2213:2225	the knowledge of glycomic features distinct from those of humans	2213:2276	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	4	107	theme	diagnostic	1122:1131	arg1	ions					1137:1140	diagnostic MS2 ions	1122:1140	diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes	1122:1219	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	5	108	theme	negative	1287:1294	arg1	analysis					1301:1308	negative mode analysis	1287:1308	negative mode analysis of sample fractions enriched in negatively charged glycans	1287:1367	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	7	109	theme	glycome	1973:1979	arg1	atlas					1981:1985	our adult zebrafish glycome atlas	1953:1985	our adult zebrafish glycome atlas	1953:1985	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	8	110	theme	brain-related	2364:2376	arg1	studies					2397:2403	brain-related glyco-neurobiology studies	2364:2403	brain-related glyco-neurobiology studies	2364:2403	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	6	111	theme	terminal	1546:1553	arg1	HexNAc					1564:1569	terminal sulfated HexNAc	1546:1569	terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc)	1546:1685	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	4	112	theme	composite	1080:1088	arg1	charts					1090:1095	composite charts	1080:1095	composite charts	1080:1095	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	4	113	theme	Homing	899:904	arg1	variants					1237:1244	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants	899:1244	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants	899:1244	Homing in on the brain-, intestine-, and ovary-derived samples, organ-specific glycomic reference maps based on overlaid extracted ion chromatograms of resolved glycan species, and composite charts of summed intensities of diagnostic MS2 ions representing the distribution and relative abundance of each of the glycotopes and sialic acid variants were established.					
34988116	2	114	theme	extra	475:479	arg1	β4-galactosylation					481:498	extra β4-galactosylation	475:498	extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	475:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	0	115	theme	Fragment	66:73	arg1	Ions					75:78	the Characteristic Fragment Ions	47:78	the Characteristic Fragment Ions	47:78	Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.					
34988116	7	116	theme	adult	1957:1961	arg1	atlas					1981:1985	our adult zebrafish glycome atlas	1953:1985	our adult zebrafish glycome atlas	1953:1985	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	2	117	theme	Galβ1-4GlcNAc	531:543	arg1	block					522:526	the basic building block	503:526	the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	503:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	2	118	theme	zebrafish	403:411	arg1	glycotopes					413:422	adult zebrafish glycotopes	397:422	adult zebrafish glycotopes	397:422	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	2	119	from	presence	463:470	arg1	block					522:526	the basic building block	503:526	the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids	503:647	Unambiguous identification of adult zebrafish glycotopes is particularly challenging due to the presence of extra β4-galactosylation on the basic building block of Galβ1-4GlcNAc that can be fucosylated and variably sialylated by N-acetyl, N-glycolyl, or deaminated neuraminic acids.					
34988116	8	120	theme	features	2239:2246	arg1	knowledge					2217:2225	the knowledge	2213:2225	the knowledge of glycomic features distinct from those of humans	2213:2276	Of particular importance is the knowledge of glycomic features distinct from those of humans when using adult zebrafish as an alternative vertebrate model, rather than mouse, for brain-related glyco-neurobiology studies.					
34988116	3	121	theme	zebrafish	752:760	arg1	O-glycans					733:741	O-glycans	733:741	O-glycans	733:741	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	3	121	theme	zebrafish	752:760	arg1	N-					726:727	various organ-specific N-	703:727	various organ-specific N-	703:727	Building on previous groundwork that have identified various organ-specific N- and O-glycans of adult zebrafish, we show here that all the major glycotopes of interest can be readily mapped by direct nano-LC-MS/MS analysis of permethylated glycans.					
34988116	5	122	theme	sulfated	1420:1427	arg1	glycotopes					1429:1438	sulfated glycotopes	1420:1438	sulfated glycotopes	1420:1438	Moreover, switching to negative mode analysis of sample fractions enriched in negatively charged glycans, we show, for the first time, that a full range of sulfated glycotopes is expressed in adult zebrafish.					
34988116	6	123	theme	sulfated	1555:1562	arg1	HexNAc					1564:1569	terminal sulfated HexNAc	1546:1569	terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc)	1546:1685	In particular, 3-O-sulfation of terminal Gal was commonly found, whereas terminal sulfated HexNAc as in GalNAcβ1-4GlcNAc (LacdiNAc), and 3-O-sulfated hexuronic acid as in HNK-1 epitope (SO3-3GlcAβ1-3Galβ1-4GlcNAc) were identified only in the brain and not in the intestine or ovaries analyzed in parallel.					
34988116	7	124	theme	relevance	2141:2149	arg1	search					2116:2121	search	2116:2121	search of the functional relevance of zebrafish-specific glycotopes	2116:2182	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
34988116	7	125	theme	discovery	1899:1907	arg1	work					1928:1931	this discovery mode sulfoglycomic work	1894:1931	this discovery mode sulfoglycomic work	1894:1931	Other characteristic structural features of sulfated O- and N-glycans along with their diagnostic ions detected in this discovery mode sulfoglycomic work collectively expand our adult zebrafish glycome atlas, which can now allow for a more complete navigation and probing of the underlying sulfotransferases and glycosyltransferases, in search of the functional relevance of zebrafish-specific glycotopes.					
32314017	15	0	dep	alginate	1990:1997	arg1	Glycine					2012:2018	Glycine	2012:2018	aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2)	1957:2027	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	15	0	dep	alginate	1990:1997	arg1	pH = 2					2021:2026	pH = 2	2021:2026	pH = 2	2021:2026	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	2	1	theme	carbon	355:360	arg1	nanotubes					362:370	magnetic carboxylated carbon nanotubes	333:370	magnetic carboxylated carbon nanotubes based on the π-interaction	333:397	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	7	2	theme	luminol-NaOH-H2O2	1108:1124	arg1	system					1126:1131	the luminol-NaOH-H2O2 system	1104:1131	the luminol-NaOH-H2O2 system	1104:1131	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	10	3	theme	MOFs	1402:1405	arg1	surface					1387:1393	the surface	1383:1393	the surface of the MOFs, which improves the chemiluminescence efficiency	1383:1454	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	6	4	theme	lysozyme	878:885	arg1	Determination					861:873	Determination	861:873	Determination of lysozyme	861:885	Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm.					
32314017	2	5	used	used	276:279	arg2	aptamer					264:270	Luminol-labeled aptamer	248:270	Luminol-labeled aptamer	248:270	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	2	5	used	used	276:279	arg2	tag					293:295	a signal tag	284:295	a signal tag	284:295	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	2	6	theme	signal	286:291	arg1	aptamer					264:270	Luminol-labeled aptamer	248:270	Luminol-labeled aptamer	248:270	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	2	6	theme	signal	286:291	arg1	tag					293:295	a signal tag	284:295	a signal tag	284:295	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	16	7	dep	tag	2093:2095	arg1	nanotubes					2114:2122	magnetic carbon nanotubes	2098:2122	signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA	2086:2157	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	16	7	dep	tag	2093:2095	arg1	MCNTs					2125:2129	MCNTs	2125:2129	MCNTs	2125:2129	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	16	7	dep	tag	2093:2095	arg1	cDNA					2154:2157	cDNA	2154:2157	signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA	2086:2157	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	7	8	theme	catalytic	1084:1092	arg1	effect					1094:1099	a strong catalytic effect	1075:1099	a strong catalytic effect	1075:1099	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	6	9	theme	at a	980:983	arg1	wavelength					985:994	a chemiluminescence signal at a wavelength	953:994	a chemiluminescence signal at a wavelength of 425 nm	953:1004	Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm.					
32314017	16	10	theme	Apt	2180:2182	arg1	HKUST-1					2191:2197	Apt; hemin@HKUST-1	2180:2197	Apt; hemin@HKUST-1	2180:2197	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	0	11	theme	hemin	118:122	arg1	HKUST-1					124:130	hemin@HKUST-1	118:130	hemin@HKUST-1	118:130	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	11	12	theme	HKUST-1	1483:1489	arg1	introduction					1461:1472	The introduction	1457:1472	The introduction of hemin@HKUST-1 and DNA	1457:1497	The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis.					
32314017	12	13	theme	6.00 × 10-13 mol∙L-1	1646:1665	arg1	7.14 × 10-9 mol∙L-1					1688:1706	7.14 × 10-9 mol∙L-1	1688:1706	7.14 × 10-9 mol∙L-1	1688:1706	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	12	13	theme	6.00 × 10-13 mol∙L-1	1646:1665	arg1	-1.25 × 10-2 U∙mg-1					1667:1685	1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1	1626:1685	1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1)	1626:1707	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	4	14	theme	hemin	648:652	arg1	HKUST-1					654:660	the hemin@HKUST-1	644:660	the hemin@HKUST-1	644:660	When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released.					
32314017	15	15	theme	magnetic	1974:1981	arg1	alginate					1990:1997	aptamer-modified magnetic sodium alginate	1957:1997	aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2)	1957:2027	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	15	15	theme	magnetic	1974:1981	arg1	M-Alg-Apt					2000:2008	M-Alg-Apt	2000:2008	M-Alg-Apt	2000:2008	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	0	16	theme	HKUST-1	124:130	arg1	use					69:71	the use	65:71	the use of magnetic alginate-aptamer composition and hemin@HKUST-1	65:130	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	2	17	theme	Luminol-labeled	248:262	arg1	aptamer					264:270	Luminol-labeled aptamer	248:270	Luminol-labeled aptamer	248:270	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	2	17	theme	Luminol-labeled	248:262	arg1	tag					293:295	a signal tag	284:295	a signal tag	284:295	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	7	18	theme	hemin	1057:1061	arg1	HKUST-1					1063:1069	the synthesized hemin@HKUST-1	1041:1069	the synthesized hemin@HKUST-1	1041:1069	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	6	19	theme	luminol-labeled	917:931	arg1	aptamer					933:939	the luminol-labeled aptamer	913:939	the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm	913:1004	Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm.					
32314017	9	20	theme	@	1282:1282	arg1	HKUST-1					1283:1289	hemin@HKUST-1	1277:1289	hemin@HKUST-1	1277:1289	The complementary pairing allowed the luminol to be immobilized on the surface of hemin@HKUST-1.					
32314017	1	21	theme	aptamer-functionalized	142:163	arg1	hydrogel					183:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme.					
32314017	1	22	theme	alginate	174:181	arg1	hydrogel					183:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme.					
32314017	16	23	theme	adsorption	2135:2144	arg1	matrix					2146:2151	adsorption matrix	2135:2151	adsorption matrix	2135:2151	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	11	24	theme	chemiluminescence	1521:1537	arg1	analysis					1539:1546	chemiluminescence analysis	1521:1546	chemiluminescence analysis	1521:1546	The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis.					
32314017	0	25	theme	magnetic	76:83	arg1	composition					102:112	magnetic alginate-aptamer composition	76:112	magnetic alginate-aptamer composition	76:112	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	0	26	theme	composition	102:112	arg1	use					69:71	the use	65:71	the use of magnetic alginate-aptamer composition and hemin@HKUST-1	65:130	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	16	27	theme	signal	2086:2091	arg1	tag					2093:2095	signal tag	2086:2095	signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA	2086:2157	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	10	28	theme	short-lived	1326:1336	arg1	species					1354:1360	short-lived reactive oxygen species	1326:1360	short-lived reactive oxygen species	1326:1360	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	5	29	theme	signal	815:820	arg1	tag					822:824	more signal tag	810:824	more signal tag	810:824	The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution.					
32314017	14	30	theme	Schematic	1875:1883	arg1	presentation					1885:1896	Graphical abstract Schematic presentation	1856:1896	Graphical abstract Schematic presentation of chemiluminescence assay.	1856:1924	Graphical abstract Schematic presentation of chemiluminescence assay.					
32314017	7	31	contain	had	1071:1073	arg1	HKUST-1					1063:1069	the synthesized hemin@HKUST-1	1041:1069	the synthesized hemin@HKUST-1	1041:1069	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	7	31	contain	had	1071:1073	arg2	effect					1094:1099	a strong catalytic effect	1075:1099	a strong catalytic effect	1075:1099	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	11	32	theme	@	1482:1482	arg1	HKUST-1					1483:1489	hemin@HKUST-1	1477:1489	hemin@HKUST-1	1477:1489	The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis.					
32314017	5	33	theme	carboxylated	771:782	arg1	nanotube					791:798	the magnetic carboxylated carbon nanotube	758:798	the magnetic carboxylated carbon nanotube	758:798	The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution.					
32314017	14	34	theme	Graphical	1856:1864	arg1	presentation					1885:1896	Graphical abstract Schematic presentation	1856:1896	Graphical abstract Schematic presentation of chemiluminescence assay.	1856:1924	Graphical abstract Schematic presentation of chemiluminescence assay.					
32314017	16	35	theme	carbon	2107:2112	arg1	MCNTs					2125:2129	MCNTs	2125:2129	MCNTs	2125:2129	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	16	35	theme	carbon	2107:2112	arg1	nanotubes					2114:2122	magnetic carbon nanotubes	2098:2122	signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA	2086:2157	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	2	36	theme	carboxylated	342:353	arg1	nanotubes					362:370	magnetic carboxylated carbon nanotubes	333:370	magnetic carboxylated carbon nanotubes based on the π-interaction	333:397	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	0	37	theme	lysozyme	47:54	arg1	determination					30:42	determination	30:42	determination of lysozyme	30:54	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	10	38	theme	oxygen	1347:1352	arg1	species					1354:1360	short-lived reactive oxygen species	1326:1360	short-lived reactive oxygen species	1326:1360	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	1	39	theme	lysozyme	238:245	arg1	enrichment					224:233	enrichment	224:233	enrichment	224:233	Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme.					
32314017	1	39	theme	lysozyme	238:245	arg1	separation					209:218	separation	209:218	separation	209:218	Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme.					
32314017	3	40	theme	carboxylated	524:535	arg1	nanotubes					544:552	the magnetic carboxylated carbon nanotubes	511:552	the magnetic carboxylated carbon nanotubes	511:552	When lysozyme was added, the aptamer specifically binds to the lysozyme, causing the signal tag to detach from the magnetic carboxylated carbon nanotubes.					
32314017	4	41	theme	complementary	602:614	arg1	strand					623:628	the complementary single strand	598:628	the complementary single strand of aptamer	598:639	When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released.					
32314017	8	42	theme	The chemiluminescence	1134:1154	arg1	signal					1156:1161	The chemiluminescence signal	1134:1161	The chemiluminescence signal	1134:1161	The chemiluminescence signal was increased nearly 100 times.					
32314017	9	43	theme	complementary	1199:1211	arg1	pairing					1213:1219	The complementary pairing	1195:1219	The complementary pairing	1195:1219	The complementary pairing allowed the luminol to be immobilized on the surface of hemin@HKUST-1.					
32314017	3	44	theme	carbon	537:542	arg1	nanotubes					544:552	the magnetic carboxylated carbon nanotubes	511:552	the magnetic carboxylated carbon nanotubes	511:552	When lysozyme was added, the aptamer specifically binds to the lysozyme, causing the signal tag to detach from the magnetic carboxylated carbon nanotubes.					
32314017	14	45	theme	assay	1919:1923	arg1	presentation					1885:1896	Graphical abstract Schematic presentation	1856:1896	Graphical abstract Schematic presentation of chemiluminescence assay.	1856:1924	Graphical abstract Schematic presentation of chemiluminescence assay.					
32314017	3	46	theme	signal	485:490	arg1	tag					492:494	the signal tag	481:494	the signal tag	481:494	When lysozyme was added, the aptamer specifically binds to the lysozyme, causing the signal tag to detach from the magnetic carboxylated carbon nanotubes.					
32314017	3	47	theme	magnetic	515:522	arg1	nanotubes					544:552	the magnetic carboxylated carbon nanotubes	511:552	the magnetic carboxylated carbon nanotubes	511:552	When lysozyme was added, the aptamer specifically binds to the lysozyme, causing the signal tag to detach from the magnetic carboxylated carbon nanotubes.					
32314017	4	48	theme	@	653:653	arg1	HKUST-1					654:660	the hemin@HKUST-1	644:660	the hemin@HKUST-1	644:660	When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released.					
32314017	13	49	theme	lysozyme	1805:1812	arg1	recovery					1793:1800	The recovery	1789:1800	The recovery of lysozyme	1789:1812	The recovery of lysozyme in spiked saliva samples was 97.4-102.8%.					
32314017	7	50	theme	strong	1077:1082	arg1	effect					1094:1099	a strong catalytic effect	1075:1099	a strong catalytic effect	1075:1099	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	10	51	theme	chemiluminescence	1427:1443	arg1	efficiency					1445:1454	the chemiluminescence efficiency	1423:1454	the chemiluminescence efficiency	1423:1454	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	13	52	theme	saliva	1824:1829	arg1	%					1853:1853	saliva samples was 97.4-102.8%	1824:1853	saliva samples was 97.4-102.8%	1824:1853	The recovery of lysozyme in spiked saliva samples was 97.4-102.8%.					
32314017	0	53	theme	@	123:123	arg1	HKUST-1					124:130	hemin@HKUST-1	118:130	hemin@HKUST-1	118:130	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	2	54	theme	signal	306:311	arg1	tag					313:315	the signal tag	302:315	the signal tag	302:315	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	6	55	theme	signal	973:978	arg1	wavelength					985:994	a chemiluminescence signal at a wavelength	953:994	a chemiluminescence signal at a wavelength of 425 nm	953:1004	Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm.					
32314017	14	56	theme	chemiluminescence	1901:1917	arg1	assay					1919:1923	chemiluminescence assay	1901:1923	chemiluminescence assay	1901:1923	Graphical abstract Schematic presentation of chemiluminescence assay.					
32314017	4	57	theme	aptamer	633:639	arg1	strand					623:628	the complementary single strand	598:628	the complementary single strand of aptamer	598:639	When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released.					
32314017	15	58	theme	aptamer-modified	1957:1972	arg1	alginate					1990:1997	aptamer-modified magnetic sodium alginate	1957:1997	aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2)	1957:2027	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	15	58	theme	aptamer-modified	1957:1972	arg1	M-Alg-Apt					2000:2008	M-Alg-Apt	2000:2008	M-Alg-Apt	2000:2008	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	7	59	theme	@	1062:1062	arg1	HKUST-1					1063:1069	the synthesized hemin@HKUST-1	1041:1069	the synthesized hemin@HKUST-1	1041:1069	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	12	60	theme	linear	1610:1615	arg1	range					1617:1621	linear range	1610:1621	linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1)	1610:1707	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	15	61	theme	sodium	1983:1988	arg1	alginate					1990:1997	aptamer-modified magnetic sodium alginate	1957:1997	aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2)	1957:2027	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	15	61	theme	sodium	1983:1988	arg1	M-Alg-Apt					2000:2008	M-Alg-Apt	2000:2008	M-Alg-Apt	2000:2008	Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys.					
32314017	7	62	theme	synthesized	1045:1055	arg1	HKUST-1					1063:1069	the synthesized hemin@HKUST-1	1041:1069	the synthesized hemin@HKUST-1	1041:1069	It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H2O2 system.					
32314017	12	63	theme	1.05 × 10-6 U∙mg-1	1626:1643	arg1	7.14 × 10-9 mol∙L-1					1688:1706	7.14 × 10-9 mol∙L-1	1688:1706	7.14 × 10-9 mol∙L-1	1688:1706	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	12	63	theme	1.05 × 10-6 U∙mg-1	1626:1643	arg1	-1.25 × 10-2 U∙mg-1					1667:1685	1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1	1626:1685	1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1)	1626:1707	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	5	64	theme	released	690:697	arg1	lysozyme					699:706	The released lysozyme	686:706	The released lysozyme	686:706	The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution.					
32314017	9	65	theme	hemin	1277:1281	arg1	HKUST-1					1283:1289	hemin@HKUST-1	1277:1289	hemin@HKUST-1	1277:1289	The complementary pairing allowed the luminol to be immobilized on the surface of hemin@HKUST-1.					
32314017	11	66	theme	DNA	1495:1497	arg1	introduction					1461:1472	The introduction	1457:1472	The introduction of hemin@HKUST-1 and DNA	1457:1497	The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis.					
32314017	9	67	theme	HKUST-1	1283:1289	arg1	surface					1266:1272	the surface	1262:1272	the surface of hemin@HKUST-1	1262:1289	The complementary pairing allowed the luminol to be immobilized on the surface of hemin@HKUST-1.					
32314017	1	68	theme	Lysozyme	133:140	arg1	hydrogel					183:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme.					
32314017	13	69	theme	samples was	1831:1841	arg1	%					1853:1853	saliva samples was 97.4-102.8%	1824:1853	saliva samples was 97.4-102.8%	1824:1853	The recovery of lysozyme in spiked saliva samples was 97.4-102.8%.					
32314017	11	70	theme	analysis	1539:1546	arg1	defects					1510:1516	the defects	1506:1516	the defects of chemiluminescence analysis	1506:1546	The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis.					
32314017	10	71	dep	generation	1296:1305	arg1	The					1292:1294	The	1292:1294	The	1292:1294	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	1	72	theme	magnetic	165:172	arg1	hydrogel					183:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel	133:190	Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme.					
32314017	12	73	theme	chemiluminescence	1553:1569	arg1	assay					1571:1575	The chemiluminescence assay	1549:1575	The chemiluminescence assay	1549:1575	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	12	73	theme	chemiluminescence	1553:1569	arg1	able					1581:1584	able	1581:1584	able	1581:1584	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	0	74	theme	alginate-aptamer	85:100	arg1	composition					102:112	magnetic alginate-aptamer composition	76:112	magnetic alginate-aptamer composition	76:112	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	6	75	theme	425 nm	999:1004	arg1	wavelength					985:994	a chemiluminescence signal at a wavelength	953:994	a chemiluminescence signal at a wavelength of 425 nm	953:1004	Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm.					
32314017	16	76	theme	Luminol-modified	2048:2063	arg1	Apt-luminol					2070:2080	Apt-luminol	2070:2080	Apt-luminol	2070:2080	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	16	76	theme	Luminol-modified	2048:2063	arg1	Apt					2065:2067	Luminol-modified Apt	2048:2067	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA	2048:2157	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	16	77	theme	@	2190:2190	arg1	HKUST-1					2191:2197	Apt; hemin@HKUST-1	2180:2197	Apt; hemin@HKUST-1	2180:2197	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	0	78	theme	chemiluminescence	2:18	arg1	assay					20:24	A chemiluminescence assay	0:24	A chemiluminescence assay for determination of lysozyme	0:54	A chemiluminescence assay for determination of lysozyme based on the use of magnetic alginate-aptamer composition and hemin@HKUST-1.					
32314017	6	79	theme	a chemiluminescence	953:971	arg1	wavelength					985:994	a chemiluminescence signal at a wavelength	953:994	a chemiluminescence signal at a wavelength of 425 nm	953:1004	Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm.					
32314017	12	80	theme	detection	1714:1722	arg1	3.50 × 10-7 U∙mg-1					1734:1751	3.50 × 10-7 U∙mg-1	1734:1751	3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99)	1734:1786	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	12	80	theme	detection	1714:1722	arg1	limit					1724:1728	the detection limit	1710:1728	the detection limit	1710:1728	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	10	81	theme	reactive	1338:1345	arg1	species					1354:1360	short-lived reactive oxygen species	1326:1360	short-lived reactive oxygen species	1326:1360	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	12	82	theme	-1.25 × 10-2 U∙mg-1	1667:1685	arg1	range					1617:1621	linear range	1610:1621	linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1)	1610:1707	The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10-6 U∙mg-1 (6.00 × 10-13 mol∙L-1)-1.25 × 10-2 U∙mg-1 (7.14 × 10-9 mol∙L-1); the detection limit was 3.50 × 10-7 U∙mg-1 (2.00 × 10-13 mol∙L-1) (R2 = 0.99).					
32314017	4	83	theme	aptamer/lysozyme	564:579	arg1	complex					581:587	the aptamer/lysozyme complex	560:587	the aptamer/lysozyme complex	560:587	When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released.					
32314017	11	84	theme	hemin	1477:1481	arg1	HKUST-1					1483:1489	hemin@HKUST-1	1477:1489	hemin@HKUST-1	1477:1489	The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis.					
32314017	5	85	theme	magnetic	762:769	arg1	nanotube					791:798	the magnetic carboxylated carbon nanotube	758:798	the magnetic carboxylated carbon nanotube	758:798	The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution.					
32314017	14	86	theme	abstract	1866:1873	arg1	presentation					1885:1896	Graphical abstract Schematic presentation	1856:1896	Graphical abstract Schematic presentation of chemiluminescence assay.	1856:1924	Graphical abstract Schematic presentation of chemiluminescence assay.					
32314017	16	87	theme	hemin	2185:2189	arg1	HKUST-1					2191:2197	Apt; hemin@HKUST-1	2180:2197	Apt; hemin@HKUST-1	2180:2197	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	5	88	theme	signal	735:740	arg1	tag					742:744	the signal tag	731:744	the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution	731:858	The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution.					
32314017	5	89	theme	carbon	784:789	arg1	nanotube					791:798	the magnetic carboxylated carbon nanotube	758:798	the magnetic carboxylated carbon nanotube	758:798	The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution.					
32314017	16	90	theme	magnetic	2098:2105	arg1	MCNTs					2125:2129	MCNTs	2125:2129	MCNTs	2125:2129	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	16	90	theme	magnetic	2098:2105	arg1	nanotubes					2114:2122	magnetic carbon nanotubes	2098:2122	signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA	2086:2157	Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.					
32314017	10	91	theme	species	1354:1360	arg1	generation					1296:1305	generation	1296:1305	generation	1296:1305	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	10	91	theme	species	1354:1360	arg1	consumption					1311:1321	consumption	1311:1321	consumption	1311:1321	The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency.					
32314017	2	92	theme	magnetic	333:340	arg1	nanotubes					362:370	magnetic carboxylated carbon nanotubes	333:370	magnetic carboxylated carbon nanotubes based on the π-interaction	333:397	Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction.					
32314017	4	93	theme	single	616:621	arg1	strand					623:628	the complementary single strand	598:628	the complementary single strand of aptamer	598:639	When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released.					
32373881	4	0	theme	GSH/Cys/Hcy	1031:1041	arg1	discrimination					992:1005	discrimination	992:1005	discrimination	992:1005	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	4	0	theme	GSH/Cys/Hcy	1031:1041	arg1	detection					1011:1019	detection	1011:1019	detection	1011:1019	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	3	1	theme	@	711:711	arg1	AuNPs					712:716	R6G-β-CD@AuNPs	703:716	R6G-β-CD@AuNPs	703:716	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	4	2	dep	discrimination	992:1005	arg1	the					988:990	the	988:990	the	988:990	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	6	3	theme	AuNPs	1383:1387	arg1	platform					1397:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	3	4	theme	pH	580:581	arg1	value					583:587	the pH value	576:587	the pH value of the tested solution	576:610	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	4	theme	pH	580:581	arg1	point					646:650	approximately the isoelectric point	616:650	approximately the isoelectric point (pI) of the biothiol	616:671	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	5	from	biothiol	844:851	arg1	surfaces					860:867	the surfaces	856:867	the surfaces of AuNPs	856:876	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	6	theme	biothiol	768:775	arg1	introduction					748:759	the introduction	744:759	the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond	744:901	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	1	7	theme	amino	213:217	arg1	GSH/Cys/Hcy					276:286	GSH/Cys/Hcy	276:286	GSH/Cys/Hcy	276:286	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	1	7	theme	amino	213:217	arg1	acids					219:223	mercapto amino acids	204:223	mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency	204:308	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	7	8	theme	great	1605:1609	arg1	potential					1611:1619	great potential	1605:1619	great potential for biothiol-related therapeutic drug monitoring	1605:1668	The pI-mediated sensing platform exhibits great potential for biothiol-related therapeutic drug monitoring.					
32373881	4	9	theme	pI-mediated	908:918	arg1	process					944:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	4	10	theme	5.5	1071:1073	arg1	values					1053:1058	the pH values	1046:1058	the pH values	1046:1058	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	0	11	theme	nanoparticles	146:158	arg1	exchange					105:112	isoelectric point-mediated surface ligand exchange	63:112	isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles	63:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	3	12	theme	tested	596:601	arg1	solution					603:610	the tested solution	592:610	the tested solution	592:610	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	6	13	from	samples	1554:1560	arg1	detection					1516:1524	the detection	1512:1524	the detection of biothiols in human serum samples	1512:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	4	14	theme	ligand	928:933	arg1	process					944:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	6	15	theme	serum	1548:1552	arg1	samples					1554:1560	human serum samples	1542:1560	human serum samples	1542:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	7	16	theme	pI-mediated	1567:1577	arg1	platform					1587:1594	The pI-mediated sensing platform	1563:1594	The pI-mediated sensing platform	1563:1594	The pI-mediated sensing platform exhibits great potential for biothiol-related therapeutic drug monitoring.					
32373881	1	17	from	acids	219:223	arg1	essential					313:321	essential	313:321	essential	313:321	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	1	17	from	acids	219:223	arg1	discrimination					165:178	The discrimination	161:178	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency	161:308	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	6	18	from	biothiols	1529:1537	arg1	samples					1554:1560	human serum samples	1542:1560	human serum samples	1542:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	4	19	from	values	1053:1058	arg1	discrimination					992:1005	discrimination	992:1005	discrimination	992:1005	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	4	19	from	values	1053:1058	arg1	detection					1011:1019	detection	1011:1019	detection	1011:1019	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	0	20	theme	isoelectric	63:73	arg1	exchange					105:112	isoelectric point-mediated surface ligand exchange	63:112	isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles	63:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	1	21	theme	cysteamine	183:192	arg1	CA					195:196	cysteamine (CA)	183:197	cysteamine (CA)	183:197	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	0	22	theme	surface	90:96	arg1	exchange					105:112	isoelectric point-mediated surface ligand exchange	63:112	isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles	63:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	5	23	theme	optimized	1100:1108	arg1	conditions					1110:1119	the optimized conditions	1096:1119	the optimized conditions	1096:1119	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	6	24	from	detection	1516:1524	arg1	samples					1554:1560	human serum samples	1542:1560	human serum samples	1542:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	5	25	theme	detection	1239:1247	arg1	limits					1249:1254	the detection limits	1235:1254	the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively	1235:1338	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	3	26	theme	R6G-β-CD	818:825	arg1	composite					827:835	the R6G-β-CD composite	814:835	the R6G-β-CD composite	814:835	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	0	27	from	acids	49:53	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.	0:159	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	0	28	theme	β-cyclodextrin-modified	117:139	arg1	nanoparticles					146:158	β-cyclodextrin-modified gold nanoparticles	117:158	β-cyclodextrin-modified gold nanoparticles	117:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	0	29	theme	amino	43:47	arg1	acids					49:53	mercapto amino acids	34:53	mercapto amino acids	34:53	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	3	30	theme	stronger	884:891	arg1	bond					898:901	a stronger S-Au bond	882:901	a stronger S-Au bond	882:901	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	1	31	theme	high	294:297	arg1	efficiency					299:308	high efficiency	294:308	high efficiency	294:308	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	6	32	from	cholesterol	1440:1450	arg1	interferences					1421:1433	the interferences	1417:1433	the interferences from cholesterol	1417:1450	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	7	33	theme	biothiol-related	1625:1640	arg1	monitoring					1659:1668	biothiol-related therapeutic drug monitoring	1625:1668	biothiol-related therapeutic drug monitoring	1625:1668	The pI-mediated sensing platform exhibits great potential for biothiol-related therapeutic drug monitoring.					
32373881	2	34	theme	encapsulated	482:493	arg1	6G					505:506	encapsulated rhodamine 6G	482:506	encapsulated rhodamine 6G (R6G-β-CD@AuNPs)	482:523	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	2	34	theme	encapsulated	482:493	arg1	AuNPs					518:522	R6G-β-CD@AuNPs	509:522	R6G-β-CD@AuNPs	509:522	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	0	35	theme	cysteamine	18:27	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.	0:159	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	2	36	theme	synthesized	436:446	arg1	AuNPs					472:476	one-pot synthesized β-cyclodextrin-modified AuNPs	428:476	one-pot synthesized β-cyclodextrin-modified AuNPs	428:476	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	0	37	theme	mercapto	34:41	arg1	acids					49:53	mercapto amino acids	34:53	mercapto amino acids	34:53	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	2	38	theme	R6G-β-CD	509:516	arg1	6G					505:506	encapsulated rhodamine 6G	482:506	encapsulated rhodamine 6G (R6G-β-CD@AuNPs)	482:523	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	2	38	theme	R6G-β-CD	509:516	arg1	AuNPs					518:522	R6G-β-CD@AuNPs	509:522	R6G-β-CD@AuNPs	509:522	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	3	39	theme	biothiol	664:671	arg1	value					583:587	the pH value	576:587	the pH value of the tested solution	576:610	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	39	theme	biothiol	664:671	arg1	pI					653:654	pI	653:654	pI	653:654	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	39	theme	biothiol	664:671	arg1	point					646:650	approximately the isoelectric point	616:650	approximately the isoelectric point (pI) of the biothiol	616:671	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	5	40	theme	enhanced	1126:1133	arg1	intensity					1148:1156	the enhanced fluorescence intensity	1122:1156	the enhanced fluorescence intensity	1122:1156	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	3	41	theme	R6G-β-CD	703:710	arg1	AuNPs					712:716	R6G-β-CD@AuNPs	703:716	R6G-β-CD@AuNPs	703:716	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	1	42	from	biothiols	333:341	arg1	environment					362:372	a physiological environment	346:372	a physiological environment	346:372	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	6	43	theme	sensing	1389:1395	arg1	platform					1397:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	5	44	theme	linear	1175:1180	arg1	response					1182:1189	a good linear response	1168:1189	a good linear response to the concentration of each biothiol	1168:1227	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	4	45	theme	pH	1050:1051	arg1	values					1053:1058	the pH values	1046:1058	the pH values	1046:1058	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	3	46	theme	AuNPs	712:716	arg1	fluorescence					687:698	the quenched fluorescence	674:698	the quenched fluorescence of R6G-β-CD@AuNPs	674:716	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	6	47	theme	@	1382:1382	arg1	platform					1397:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	6	48	theme	high	1491:1494	arg1	selectivity					1496:1506	high selectivity	1491:1506	high selectivity for the detection of biothiols in human serum samples	1491:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	3	49	theme	solution	603:610	arg1	value					583:587	the pH value	576:587	the pH value of the tested solution	576:610	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	49	theme	solution	603:610	arg1	point					646:650	approximately the isoelectric point	616:650	approximately the isoelectric point (pI) of the biothiol	616:671	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	1	50	theme	mercapto	204:211	arg1	GSH/Cys/Hcy					276:286	GSH/Cys/Hcy	276:286	GSH/Cys/Hcy	276:286	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	1	50	theme	mercapto	204:211	arg1	acids					219:223	mercapto amino acids	204:223	mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency	204:308	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	6	51	theme	host-guest	1455:1464	arg1	interactions					1466:1477	host-guest interactions	1455:1477	host-guest interactions	1455:1477	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	7	52	theme	sensing	1579:1585	arg1	platform					1587:1594	The pI-mediated sensing platform	1563:1594	The pI-mediated sensing platform	1563:1594	The pI-mediated sensing platform exhibits great potential for biothiol-related therapeutic drug monitoring.					
32373881	1	53	theme	CA	195:196	arg1	essential					313:321	essential	313:321	essential	313:321	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	1	53	theme	CA	195:196	arg1	discrimination					165:178	The discrimination	161:178	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency	161:308	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	3	54	theme	isoelectric	634:644	arg1	value					583:587	the pH value	576:587	the pH value of the tested solution	576:610	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	54	theme	isoelectric	634:644	arg1	pI					653:654	pI	653:654	pI	653:654	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	3	54	theme	isoelectric	634:644	arg1	point					646:650	approximately the isoelectric point	616:650	approximately the isoelectric point (pI) of the biothiol	616:671	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	4	55	theme	surface	920:926	arg1	process					944:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	6	56	theme	human	1542:1546	arg1	samples					1554:1560	human serum samples	1542:1560	human serum samples	1542:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	6	57	theme	biothiols	1529:1537	arg1	detection					1516:1524	the detection	1512:1524	the detection of biothiols in human serum samples	1512:1560	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	5	58	theme	CA	1259:1260	arg1	limits					1249:1254	the detection limits	1235:1254	the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively	1235:1338	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	0	59	theme	point-mediated	75:88	arg1	exchange					105:112	isoelectric point-mediated surface ligand exchange	63:112	isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles	63:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	2	60	theme	covalent	533:540	arg1	modification					542:553	covalent modification	533:553	covalent modification	533:553	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	0	61	theme	ligand	98:103	arg1	exchange					105:112	isoelectric point-mediated surface ligand exchange	63:112	isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles	63:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	1	62	theme	physiological	348:360	arg1	environment					362:372	a physiological environment	346:372	a physiological environment	346:372	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	4	63	theme	first	973:977	arg1	time					979:982	the first time	969:982	the first time	969:982	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	3	64	theme	AuNPs	872:876	arg1	surfaces					860:867	the surfaces	856:867	the surfaces of AuNPs	856:876	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	4	65	theme	exchange	935:942	arg1	process					944:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process	904:950	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	5	66	theme	Hcy	1276:1278	arg1	limits					1249:1254	the detection limits	1235:1254	the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively	1235:1338	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	3	67	theme	composite	827:835	arg1	replacement					799:809	the replacement	795:809	the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond	795:901	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	0	68	theme	gold	141:144	arg1	nanoparticles					146:158	β-cyclodextrin-modified gold nanoparticles	117:158	β-cyclodextrin-modified gold nanoparticles	117:158	Discrimination of cysteamine from mercapto amino acids through isoelectric point-mediated surface ligand exchange of β-cyclodextrin-modified gold nanoparticles.					
32373881	6	69	theme	R6G-β-CD	1374:1381	arg1	platform					1397:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	the R6G-β-CD@AuNPs sensing platform	1370:1404	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	3	70	theme	S-Au	893:896	arg1	bond					898:901	a stronger S-Au bond	882:901	a stronger S-Au bond	882:901	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	2	71	theme	rhodamine	495:503	arg1	6G					505:506	encapsulated rhodamine 6G	482:506	encapsulated rhodamine 6G (R6G-β-CD@AuNPs)	482:523	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	2	71	theme	rhodamine	495:503	arg1	AuNPs					518:522	R6G-β-CD@AuNPs	509:522	R6G-β-CD@AuNPs	509:522	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	5	72	theme	GSH	1263:1265	arg1	limits					1249:1254	the detection limits	1235:1254	the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively	1235:1338	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	7	73	theme	therapeutic	1642:1652	arg1	monitoring					1659:1668	biothiol-related therapeutic drug monitoring	1625:1668	biothiol-related therapeutic drug monitoring	1625:1668	The pI-mediated sensing platform exhibits great potential for biothiol-related therapeutic drug monitoring.					
32373881	4	74	theme	CA	1024:1025	arg1	discrimination					992:1005	discrimination	992:1005	discrimination	992:1005	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	4	74	theme	CA	1024:1025	arg1	detection					1011:1019	detection	1011:1019	detection	1011:1019	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	5	75	theme	Cys	1268:1270	arg1	limits					1249:1254	the detection limits	1235:1254	the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively	1235:1338	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	2	76	theme	β-cyclodextrin-modified	448:470	arg1	AuNPs					472:476	one-pot synthesized β-cyclodextrin-modified AuNPs	428:476	one-pot synthesized β-cyclodextrin-modified AuNPs	428:476	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	6	77	theme	ingenious	1345:1353	arg1	fabrication					1355:1365	The ingenious fabrication	1341:1365	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform	1341:1404	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	5	78	theme	good	1170:1173	arg1	response					1182:1189	a good linear response	1168:1189	a good linear response to the concentration of each biothiol	1168:1227	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	1	79	with	acids	219:223	arg1	efficiency					299:308	high efficiency	294:308	high efficiency	294:308	The discrimination of cysteamine (CA) from mercapto amino acids (including glutathione, cysteine and homocysteine, GSH/Cys/Hcy) with high efficiency is essential to detect biothiols in a physiological environment.					
32373881	2	80	theme	@	517:517	arg1	6G					505:506	encapsulated rhodamine 6G	482:506	encapsulated rhodamine 6G (R6G-β-CD@AuNPs)	482:523	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	2	80	theme	@	517:517	arg1	AuNPs					518:522	R6G-β-CD@AuNPs	509:522	R6G-β-CD@AuNPs	509:522	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	2	81	theme	one-pot	428:434	arg1	AuNPs					472:476	one-pot synthesized β-cyclodextrin-modified AuNPs	428:476	one-pot synthesized β-cyclodextrin-modified AuNPs	428:476	Herein, a nano-assembly was constructed by combining one-pot synthesized β-cyclodextrin-modified AuNPs and encapsulated rhodamine 6G (R6G-β-CD@AuNPs) without covalent modification.					
32373881	5	82	theme	fluorescence	1135:1146	arg1	intensity					1148:1156	the enhanced fluorescence intensity	1122:1156	the enhanced fluorescence intensity	1122:1156	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
32373881	3	83	theme	quenched	678:685	arg1	fluorescence					687:698	the quenched fluorescence	674:698	the quenched fluorescence of R6G-β-CD@AuNPs	674:716	Interestingly, when the pH value of the tested solution was approximately the isoelectric point (pI) of the biothiol, the quenched fluorescence of R6G-β-CD@AuNPs was obviously restored by the introduction of the biothiol, which was due to the replacement of the R6G-β-CD composite by the biothiol on the surfaces of AuNPs via a stronger S-Au bond.					
32373881	7	84	theme	drug	1654:1657	arg1	monitoring					1659:1668	biothiol-related therapeutic drug monitoring	1625:1668	biothiol-related therapeutic drug monitoring	1625:1668	The pI-mediated sensing platform exhibits great potential for biothiol-related therapeutic drug monitoring.					
32373881	4	85	theme	9.5	1063:1065	arg1	values					1053:1058	the pH values	1046:1058	the pH values	1046:1058	The pI-mediated surface ligand exchange process was designed for the first time for the discrimination and detection of CA and GSH/Cys/Hcy at the pH values of 9.5 and 5.5, respectively.					
32373881	6	86	theme	platform	1397:1404	arg1	fabrication					1355:1365	The ingenious fabrication	1341:1365	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform	1341:1404	The ingenious fabrication of the R6G-β-CD@AuNPs sensing platform eliminated the interferences from cholesterol by host-guest interactions and enabled high selectivity for the detection of biothiols in human serum samples.					
32373881	5	87	theme	biothiol	1220:1227	arg1	concentration					1198:1210	the concentration	1194:1210	the concentration of each biothiol	1194:1227	Under the optimized conditions, the enhanced fluorescence intensity displayed a good linear response to the concentration of each biothiol, with the detection limits of CA, GSH, Cys and Hcy calculated as 66.8, 417.6, 652.7 and 603.4 nM, respectively.					
33442506	6	0	from	present	870:876	arg1	LSPs					889:892	all the LSPs	881:892	all the LSPs	881:892	Also, fucose and xylose were present in all the LSPs.					
33442506	7	1	from	5 mg/mL	1078:1084	arg1	%					1073:1073	65.26 ± 1.74%	1061:1073	65.26 ± 1.74% at 5 mg/mL	1061:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	1	from	5 mg/mL	1078:1084	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	1	from	5 mg/mL	1078:1084	arg1	%					1018:1018	78.93 ± 0.42%	1006:1018	78.93 ± 0.42% at 3 mg/mL	1006:1029	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	1	from	5 mg/mL	1078:1084	arg1	%					1044:1044	79.16 ± 1.43%	1032:1044	79.16 ± 1.43% at 3 mg/mL	1032:1055	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	5	2	theme	different	777:785	arg1	proportions					787:797	different proportions	777:797	different proportions	777:797	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	3	3	theme	SR-FTIR	468:474	arg1	spectra					477:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	0	4	theme	fruiting	82:89	arg1	bodies					91:96	the fruiting bodies	78:96	the fruiting bodies of Lentinus squarrosulus	78:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	0	5	from	composition	11:21	arg1	bodies					91:96	the fruiting bodies	78:96	the fruiting bodies of Lentinus squarrosulus	78:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	10	6	theme	food	1597:1600	arg1	industries					1616:1625	food and medicinal industries	1597:1625	food and medicinal industries	1597:1625	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	8	7	theme	poor	1370:1373	arg1	scavenger					1380:1388	poor ABTS scavenger	1370:1388	poor ABTS scavenger	1370:1388	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	3	8	from	analysis	498:505	arg1	bio-regions					514:524	the bio-regions	510:524	the bio-regions (4000-400 cm-1)	510:540	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	3	8	from	analysis	498:505	arg1	4000-400 cm-1					527:539	4000-400 cm-1	527:539	4000-400 cm-1	527:539	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	7	9	from	3 mg/mL	1232:1238	arg1	%					1257:1257	47.24 ± 0.045%	1244:1257	47.24 ± 0.045% at 5 mg/mL	1244:1268	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	9	from	3 mg/mL	1232:1238	arg1	%					1227:1227	97.42 ± 0.76%	1215:1227	97.42 ± 0.76% at 3 mg/mL	1215:1238	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	9	from	3 mg/mL	1232:1238	arg1	%					1201:1201	98.94 ± 0.16%	1189:1201	98.94 ± 0.16% at 3 mg/mL	1189:1212	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	0	10	theme	Lentinus	101:108	arg1	squarrosulus					110:121	Lentinus squarrosulus	101:121	Lentinus squarrosulus	101:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	9	11	used	used	1466:1469	arg2	antioxidants					1482:1493	natural antioxidants	1474:1493	natural antioxidants	1474:1493	In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants.					
33442506	9	11	used	used	1466:1469	arg2	LSPs					1403:1406	LSPs	1403:1406	LSPs	1403:1406	In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants.					
33442506	7	12	dep	2,2'-azino-bis	1097:1110	arg1	ABTS					1152:1155	ABTS	1152:1155	ABTS	1152:1155	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	12	dep	2,2'-azino-bis	1097:1110	arg1	acid					1146:1149	3-ethylbenzothiazoline-6-sulfonic acid	1112:1149	3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	1112:1156	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	5	13	contain	contained	813:821	arg2	d-glucose					830:838	d-glucose	830:838	d-glucose	830:838	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	5	13	contain	contained	813:821	arg1	LSP-AP					806:811	LSP-AP	806:811	LSP-AP	806:811	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	7	14	from	5 mg/mL	1262:1268	arg1	%					1257:1257	47.24 ± 0.045%	1244:1257	47.24 ± 0.045% at 5 mg/mL	1244:1268	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	14	from	5 mg/mL	1262:1268	arg1	%					1227:1227	97.42 ± 0.76%	1215:1227	97.42 ± 0.76% at 3 mg/mL	1215:1238	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	14	from	5 mg/mL	1262:1268	arg1	%					1201:1201	98.94 ± 0.16%	1189:1201	98.94 ± 0.16% at 3 mg/mL	1189:1212	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	9	15	theme	natural	1474:1480	arg1	antioxidants					1482:1493	natural antioxidants	1474:1493	natural antioxidants	1474:1493	In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants.					
33442506	9	15	theme	natural	1474:1480	arg1	LSPs					1403:1406	LSPs	1403:1406	LSPs	1403:1406	In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants.					
33442506	7	16	from	whiles	1087:1092	arg1	activities					1173:1182	their maximum activities	1159:1182	their maximum activities	1159:1182	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	16	from	whiles	1087:1092	arg1	2,2'-azino-bis					1097:1110	2,2'-azino-bis	1097:1110	2,2'-azino-bis	1097:1110	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	3	17	from	spectra	477:483	arg1	bio-regions					514:524	the bio-regions	510:524	the bio-regions (4000-400 cm-1)	510:540	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	3	17	from	spectra	477:483	arg1	4000-400 cm-1					527:539	4000-400 cm-1	527:539	4000-400 cm-1	527:539	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	10	18	theme	extracts	1538:1545	arg1	exploitation					1501:1512	This exploitation	1496:1512	This exploitation of fungal fruiting body extracts	1496:1545	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	5	19	theme	major	689:693	arg1	monosaccharides					695:709	The major monosaccharides	685:709	The major monosaccharides in LSP-CP and LSP-HP	685:730	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	5	19	theme	major	689:693	arg1	d-galactose					736:746	d-galactose	736:746	d-galactose	736:746	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	3	20	theme	FTIR	462:465	arg1	spectra					477:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	6	21	located	present	870:876	arg2	xylose					858:863	xylose	858:863	xylose	858:863	Also, fucose and xylose were present in all the LSPs.					
33442506	6	21	located	present	870:876	arg1	LSPs					889:892	all the LSPs	881:892	all the LSPs	881:892	Also, fucose and xylose were present in all the LSPs.					
33442506	6	21	located	present	870:876	arg2	fucose					847:852	fucose	847:852	fucose	847:852	Also, fucose and xylose were present in all the LSPs.					
33442506	2	22	theme	cold	273:276	arg1	water					278:282	cold water	273:282	cold water (LSP-CP)	273:291	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	2	22	theme	cold	273:276	arg1	LSP-CP					285:290	LSP-CP	285:290	LSP-CP	285:290	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	0	23	theme	squarrosulus	110:121	arg1	bodies					91:96	the fruiting bodies	78:96	the fruiting bodies of Lentinus squarrosulus	78:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	10	24	theme	body	1533:1536	arg1	extracts					1538:1545	fungal fruiting body extracts	1517:1545	fungal fruiting body extracts	1517:1545	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	7	25	theme	%	1044:1044	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	9	26	theme	essential	1419:1427	arg1	compositions					1440:1451	essential functional compositions	1419:1451	essential functional compositions	1419:1451	In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants.					
33442506	0	27	theme	Functional	0:9	arg1	composition					11:21	Functional composition	0:21	Functional composition	0:21	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	7	28	theme	3-ethylbenzothiazoline-6-sulfonic	1112:1144	arg1	ABTS					1152:1155	ABTS	1152:1155	ABTS	1152:1155	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	28	theme	3-ethylbenzothiazoline-6-sulfonic	1112:1144	arg1	acid					1146:1149	3-ethylbenzothiazoline-6-sulfonic acid	1112:1149	3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	1112:1156	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	2	29	theme	scavenging	403:412	arg1	activities					414:423	radical scavenging activities	395:423	radical scavenging activities	395:423	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	0	30	from	property	39:46	arg1	bodies					91:96	the fruiting bodies	78:96	the fruiting bodies of Lentinus squarrosulus	78:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	10	31	theme	fungal	1517:1522	arg1	extracts					1538:1545	fungal fruiting body extracts	1517:1545	fungal fruiting body extracts	1517:1545	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	7	32	from	3 mg/mL	1206:1212	arg1	%					1257:1257	47.24 ± 0.045%	1244:1257	47.24 ± 0.045% at 5 mg/mL	1244:1268	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	32	from	3 mg/mL	1206:1212	arg1	%					1227:1227	97.42 ± 0.76%	1215:1227	97.42 ± 0.76% at 3 mg/mL	1215:1238	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	32	from	3 mg/mL	1206:1212	arg1	%					1201:1201	98.94 ± 0.16%	1189:1201	98.94 ± 0.16% at 3 mg/mL	1189:1212	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	2	33	theme	hot	294:296	arg1	LSP-HP					305:310	LSP-HP	305:310	LSP-HP	305:310	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	2	33	theme	hot	294:296	arg1	water					298:302	hot water	294:302	hot water (LSP-HP)	294:311	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	2	34	theme	LSP-AP	336:341	arg1	extractions					344:354	aqueous alkaline (LSP-AP) extractions	318:354	aqueous alkaline (LSP-AP) extractions	318:354	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	2	35	theme	radical	395:401	arg1	activities					414:423	radical scavenging activities	395:423	radical scavenging activities	395:423	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	7	36	from	3 mg/mL	1023:1029	arg1	%					1073:1073	65.26 ± 1.74%	1061:1073	65.26 ± 1.74% at 5 mg/mL	1061:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	36	from	3 mg/mL	1023:1029	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	36	from	3 mg/mL	1023:1029	arg1	%					1018:1018	78.93 ± 0.42%	1006:1018	78.93 ± 0.42% at 3 mg/mL	1006:1029	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	36	from	3 mg/mL	1023:1029	arg1	%					1044:1044	79.16 ± 1.43%	1032:1044	79.16 ± 1.43% at 3 mg/mL	1032:1055	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	10	37	theme	medicinal	1606:1614	arg1	industries					1616:1625	food and medicinal industries	1597:1625	food and medicinal industries	1597:1625	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	4	38	contain	had	626:628	arg2	lipids					630:635	lipids	630:635	lipids	630:635	All LSPs had lipids, protein, and polysaccharides such as β-glucan.					
33442506	4	38	contain	had	626:628	arg1	LSPs					621:624	All LSPs	617:624	All LSPs	617:624	All LSPs had lipids, protein, and polysaccharides such as β-glucan.					
33442506	4	38	contain	had	626:628	arg2	β-glucan					675:682	β-glucan	675:682	β-glucan	675:682	All LSPs had lipids, protein, and polysaccharides such as β-glucan.					
33442506	4	38	contain	had	626:628	arg2	polysaccharides					651:665	polysaccharides	651:665	polysaccharides	651:665	All LSPs had lipids, protein, and polysaccharides such as β-glucan.					
33442506	4	38	contain	had	626:628	arg2	protein					638:644	protein	638:644	protein	638:644	All LSPs had lipids, protein, and polysaccharides such as β-glucan.					
33442506	1	39	theme	bioactive	177:185	arg1	polysaccharides					187:201	bioactive polysaccharides	177:201	bioactive polysaccharides	177:201	Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides.					
33442506	0	40	theme	antioxidant	27:37	arg1	property					39:46	antioxidant property	27:46	antioxidant property	27:46	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	8	41	theme	whiles	1353:1358	arg1	LSP-AP					1360:1365	whiles LSP-AP	1353:1365	whiles LSP-AP	1353:1365	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	7	42	theme	%	1018:1018	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	2	43	theme	functional	367:376	arg1	compositions					378:389	their functional compositions	361:389	their functional compositions	361:389	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	7	44	theme	maximum	929:935	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	3	45	theme	PCA	489:491	arg1	analysis					498:505	PCA plot analysis	489:505	PCA plot analysis	489:505	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	0	46	from	bodies	91:96	arg1	composition					11:21	Functional composition	0:21	Functional composition	0:21	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	0	46	from	bodies	91:96	arg1	property					39:46	antioxidant property	27:46	antioxidant property	27:46	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	0	46	from	bodies	91:96	arg1	polysaccharides					57:71	crude polysaccharides	51:71	crude polysaccharides from the fruiting bodies of Lentinus squarrosulus	51:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	7	47	theme	radical	975:981	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	6	48	attach	present	870:876	arg2	xylose					858:863	xylose	858:863	xylose	858:863	Also, fucose and xylose were present in all the LSPs.					
33442506	6	48	attach	present	870:876	arg1	LSPs					889:892	all the LSPs	881:892	all the LSPs	881:892	Also, fucose and xylose were present in all the LSPs.					
33442506	6	48	attach	present	870:876	arg2	fucose					847:852	fucose	847:852	fucose	847:852	Also, fucose and xylose were present in all the LSPs.					
33442506	5	49	from	monosaccharides	695:709	arg1	LSP-CP					714:719	LSP-CP	714:719	LSP-CP	714:719	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	5	49	from	monosaccharides	695:709	arg1	LSP-HP					725:730	LSP-HP	725:730	LSP-HP	725:730	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	10	50	theme	potential	1561:1569	arg1	use					1571:1573	the potential use	1557:1573	the potential use of L. squarrosulus in food and medicinal industries	1557:1625	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	3	51	dep	differ	584:589	arg1	P < 0.05					606:613	P < 0.05	606:613	P < 0.05	606:613	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	8	52	theme	ABTS	1375:1378	arg1	scavenger					1380:1388	poor ABTS scavenger	1370:1388	poor ABTS scavenger	1370:1388	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	3	53	theme	functional	556:565	arg1	LSPs					579:582	functional composition LSPs	556:582	functional composition LSPs	556:582	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	10	54	from	use	1571:1573	arg1	industries					1616:1625	food and medicinal industries	1597:1625	food and medicinal industries	1597:1625	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	2	55	dep	polysaccharides	232:246	arg1	squarrosulus					213:224	Three L. squarrosulus crude polysaccharides (LSPs)	204:253	Three L. squarrosulus crude polysaccharides (LSPs)	204:253	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	0	56	theme	polysaccharides	57:71	arg1	composition					11:21	Functional composition	0:21	Functional composition	0:21	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	0	56	theme	polysaccharides	57:71	arg1	property					39:46	antioxidant property	27:46	antioxidant property	27:46	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	7	57	from	3 mg/mL	1049:1055	arg1	%					1073:1073	65.26 ± 1.74%	1061:1073	65.26 ± 1.74% at 5 mg/mL	1061:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	57	from	3 mg/mL	1049:1055	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	57	from	3 mg/mL	1049:1055	arg1	%					1018:1018	78.93 ± 0.42%	1006:1018	78.93 ± 0.42% at 3 mg/mL	1006:1029	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	57	from	3 mg/mL	1049:1055	arg1	%					1044:1044	79.16 ± 1.43%	1032:1044	79.16 ± 1.43% at 3 mg/mL	1032:1055	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	58	theme	scavenging	983:992	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	59	theme	maximum	1165:1171	arg1	activities					1173:1182	their maximum activities	1159:1182	their maximum activities	1159:1182	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	59	theme	maximum	1165:1171	arg1	2,2'-azino-bis					1097:1110	2,2'-azino-bis	1097:1110	2,2'-azino-bis	1097:1110	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	2	60	theme	crude	226:230	arg1	polysaccharides					232:246	crude polysaccharides	226:246	Three L. squarrosulus crude polysaccharides (LSPs)	204:253	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	2	60	theme	crude	226:230	arg1	LSPs					249:252	LSPs	249:252	LSPs	249:252	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	7	61	theme	DPPH	969:972	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	1	62	theme	Lentinus	124:131	arg1	Khao					156:159	Khao	156:159	Khao	156:159	Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides.					
33442506	1	62	theme	Lentinus	124:131	arg1	squarrosulus					133:144	Lentinus squarrosulus	124:144	Lentinus squarrosulus (Hed Khon Khao)	124:160	Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides.					
33442506	1	62	theme	Lentinus	124:131	arg1	source					167:172	a source	165:172	a source of bioactive polysaccharides	165:201	Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides.					
33442506	0	63	theme	crude	51:55	arg1	polysaccharides					57:71	crude polysaccharides	51:71	crude polysaccharides from the fruiting bodies of Lentinus squarrosulus	51:121	Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus.					
33442506	8	64	theme	good	1331:1334	arg1	scavengers					1341:1350	good ABTS scavengers	1331:1350	good ABTS scavengers	1331:1350	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	8	64	theme	good	1331:1334	arg1	LSP-HP					1320:1325	LSP-HP	1320:1325	LSP-HP	1320:1325	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	8	64	theme	good	1331:1334	arg1	LSP-CP					1309:1314	LSP-CP	1309:1314	LSP-CP	1309:1314	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	2	65	theme	L.	210:211	arg1	polysaccharides					232:246	crude polysaccharides	226:246	Three L. squarrosulus crude polysaccharides (LSPs)	204:253	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	2	65	theme	L.	210:211	arg1	LSPs					249:252	LSPs	249:252	LSPs	249:252	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	7	66	theme	1,1-diphenyl-2-	937:951	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	6	67	from	LSPs	889:892	arg1	present					870:876	present	870:876	present	870:876	Also, fucose and xylose were present in all the LSPs.					
33442506	5	68	from	proportions	787:797	arg1	d-glucose					749:757	d-glucose	749:757	d-glucose	749:757	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	5	68	from	proportions	787:797	arg1	d-mannose					764:772	d-mannose	764:772	d-mannose	764:772	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	5	68	from	proportions	787:797	arg1	d-galactose					736:746	d-galactose	736:746	d-galactose	736:746	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	5	68	from	proportions	787:797	arg1	monosaccharides					695:709	The major monosaccharides	685:709	The major monosaccharides in LSP-CP and LSP-HP	685:730	The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose.					
33442506	3	69	theme	Synchrotron	440:450	arg1	spectra					477:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	3	70	theme	plot	493:496	arg1	analysis					498:505	PCA plot analysis	489:505	PCA plot analysis	489:505	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	9	71	theme	functional	1429:1438	arg1	compositions					1440:1451	essential functional compositions	1419:1451	essential functional compositions	1419:1451	In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants.					
33442506	8	72	theme	ABTS	1336:1339	arg1	scavengers					1341:1350	good ABTS scavengers	1331:1350	good ABTS scavengers	1331:1350	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	8	72	theme	ABTS	1336:1339	arg1	LSP-HP					1320:1325	LSP-HP	1320:1325	LSP-HP	1320:1325	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	8	72	theme	ABTS	1336:1339	arg1	LSP-CP					1309:1314	LSP-CP	1309:1314	LSP-CP	1309:1314	The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger.					
33442506	10	73	theme	squarrosulus	1581:1592	arg1	use					1571:1573	the potential use	1557:1573	the potential use of L. squarrosulus in food and medicinal industries	1557:1625	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	3	74	theme	radiation	452:460	arg1	spectra					477:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra	440:483	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	7	75	theme	picrylhydrazyl	953:966	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	3	76	theme	composition	567:577	arg1	LSPs					579:582	functional composition LSPs	556:582	functional composition LSPs	556:582	Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P < 0.05).					
33442506	1	77	theme	polysaccharides	187:201	arg1	source					167:172	a source	165:172	a source of bioactive polysaccharides	165:201	Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides.					
33442506	1	77	theme	polysaccharides	187:201	arg1	squarrosulus					133:144	Lentinus squarrosulus	124:144	Lentinus squarrosulus (Hed Khon Khao)	124:160	Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides.					
33442506	2	78	theme	alkaline	326:333	arg1	extractions					344:354	aqueous alkaline (LSP-AP) extractions	318:354	aqueous alkaline (LSP-AP) extractions	318:354	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	7	79	theme	%	1073:1073	arg1	activity					994:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity	929:1001	maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL	929:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	10	80	theme	fruiting	1524:1531	arg1	extracts					1538:1545	fungal fruiting body extracts	1517:1545	fungal fruiting body extracts	1517:1545	This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.					
33442506	2	81	theme	aqueous	318:324	arg1	extractions					344:354	aqueous alkaline (LSP-AP) extractions	318:354	aqueous alkaline (LSP-AP) extractions	318:354	Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared.					
33442506	7	82	from	activity	994:1001	arg1	5 mg/mL					1078:1084	5 mg/mL	1078:1084	5 mg/mL	1078:1084	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	82	from	activity	994:1001	arg1	3 mg/mL					1049:1055	3 mg/mL	1049:1055	3 mg/mL	1049:1055	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
33442506	7	82	from	activity	994:1001	arg1	3 mg/mL					1023:1029	3 mg/mL	1023:1029	3 mg/mL	1023:1029	LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively.					
34056092	11	0	theme	bacterial	1467:1475	arg1	factor					1487:1492	a bacterial virulence factor	1465:1492	a bacterial virulence factor	1465:1492	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	11	0	theme	bacterial	1467:1475	arg1	toxin					1503:1507	cholera toxin	1495:1507	cholera toxin	1495:1507	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	11	1	with	Consistent	1376:1385	arg1	morphology					1407:1416	mucin's linear morphology	1392:1416	mucin's linear morphology	1392:1416	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	11	2	theme	virulence	1477:1485	arg1	factor					1487:1492	a bacterial virulence factor	1465:1492	a bacterial virulence factor	1465:1492	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	11	2	theme	virulence	1477:1485	arg1	toxin					1503:1507	cholera toxin	1495:1507	cholera toxin	1495:1507	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	9	3	dep	Muc2	1172:1175	arg1	mucins					1196:1201	mucins	1196:1201	mucins	1196:1201	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	6	4	theme	mucin	654:658	arg1	mimics					660:665	synthetic mucin mimics	644:665	synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin	644:736	To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin.					
34056092	12	5	from	importance	1537:1546	arg1	design					1591:1596	mucin surrogate design	1575:1596	mucin surrogate design	1575:1596	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	2	6	theme	animal	107:112	arg1	kingdom					114:120	the animal kingdom	103:120	the animal kingdom	103:120	Across the animal kingdom, this hydrogel mediates surface wetting, viscosity, and protection against microbes.					
34056092	12	7	theme	backbone	1652:1659	arg1	significance					1617:1628	the significance	1613:1628	the significance of the extended mucin backbone for inhibiting virulence	1613:1684	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	6	8	theme	synthetic	644:652	arg1	mimics					660:665	synthetic mucin mimics	644:665	synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin	644:736	To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin.					
34056092	12	9	theme	mucin	1646:1650	arg1	backbone					1652:1659	the extended mucin backbone	1633:1659	the extended mucin backbone	1633:1659	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	3	10	gly	O-glycoproteins	282:296	arg1	O-glycoproteins					282:296	mucins-high molecular weight O-glycoproteins	253:296	mucins-high molecular weight O-glycoproteins that adopt extended linear structures	253:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	3	10	gly	O-glycoproteins	282:296	arg1	components					219:228	The primary components	207:228	The primary components of mucus hydrogels	207:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	2	11	theme	wetting	154:160	arg1	surface					146:152	surface wetting	146:160	surface wetting	146:160	Across the animal kingdom, this hydrogel mediates surface wetting, viscosity, and protection against microbes.					
34056092	3	12	theme	primary	211:217	arg1	components					219:228	The primary components	207:228	The primary components of mucus hydrogels	207:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	3	12	theme	primary	211:217	arg1	O-glycoproteins					282:296	mucins-high molecular weight O-glycoproteins	253:296	mucins-high molecular weight O-glycoproteins that adopt extended linear structures	253:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	8	13	theme	linear	1056:1061	arg1	structures					1063:1072	linear structures	1056:1072	linear structures that mimic mucins	1056:1090	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	6	14	theme	glycans	706:712	arg1	display					695:701	the dense display	685:701	the dense display of glycans and morphology of mucin	685:736	To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin.					
34056092	9	15	theme	Muc2	1172:1175	arg1	micrographs					1122:1132	High-resolution atomic force micrographs	1093:1132	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins	1093:1201	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	11	16	theme	cholera	1495:1501	arg1	factor					1487:1492	a bacterial virulence factor	1465:1492	a bacterial virulence factor	1465:1492	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	11	16	theme	cholera	1495:1501	arg1	toxin					1503:1507	cholera toxin	1495:1507	cholera toxin	1495:1507	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	11	17	theme	linear	1400:1405	arg1	morphology					1407:1416	mucin's linear morphology	1392:1416	mucin's linear morphology	1392:1416	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	5	18	theme	mucins	528:533	arg1	conformation					512:523	the extended conformation	499:523	the extended conformation of mucins	499:533	We postulated that the extended conformation of mucins is critical for their ability to block microbial virulence phenotypes.					
34056092	5	18	theme	mucins	528:533	arg1	critical					538:545	critical	538:545	critical	538:545	We postulated that the extended conformation of mucins is critical for their ability to block microbial virulence phenotypes.					
34056092	12	19	theme	extended	1637:1644	arg1	backbone					1652:1659	the extended mucin backbone	1633:1659	the extended mucin backbone	1633:1659	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	3	20	theme	mucins-high	253:263	arg1	O-glycoproteins					282:296	mucins-high molecular weight O-glycoproteins	253:296	mucins-high molecular weight O-glycoproteins that adopt extended linear structures	253:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	3	20	theme	mucins-high	253:263	arg1	components					219:228	The primary components	207:228	The primary components of mucus hydrogels	207:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	4	21	theme	other	386:390	arg1	characteristics					392:406	other characteristics	386:406	other characteristics that give rise to their advantageous biological activities	386:465	Glycosylation is integral to mucin function, but other characteristics that give rise to their advantageous biological activities are unknown.					
34056092	9	22	theme	High-resolution	1093:1107	arg1	micrographs					1122:1132	High-resolution atomic force micrographs	1093:1132	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins	1093:1201	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	6	23	theme	mucin	732:736	arg1	display					695:701	the dense display	685:701	the dense display of glycans and morphology of mucin	685:736	To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin.					
34056092	3	24	theme	mucus	233:237	arg1	hydrogels					239:247	mucus hydrogels	233:247	mucus hydrogels	233:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	8	25	theme	polymers	942:949	arg1	analysis					926:933	Conformational analysis	911:933	Conformational analysis of the polymers based on allylic strain	911:973	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	0	26	theme	Stereochemical	0:13	arg1	Control					15:21	Stereochemical Control	0:21	Stereochemical Control	0:21	Stereochemical Control Yields Mucin Mimetic Polymers.					
34056092	7	27	theme	norbornene-derived	837:854	arg1	glycopolymers					856:868	substituted norbornene-derived glycopolymers	825:868	substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes	825:908	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	3	28	theme	hydrogels	239:247	arg1	components					219:228	The primary components	207:228	The primary components of mucus hydrogels	207:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	3	28	theme	hydrogels	239:247	arg1	O-glycoproteins					282:296	mucins-high molecular weight O-glycoproteins	253:296	mucins-high molecular weight O-glycoproteins that adopt extended linear structures	253:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	12	29	theme	mucin	1575:1579	arg1	design					1591:1596	mucin surrogate design	1575:1596	mucin surrogate design	1575:1596	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	6	30	theme	morphology	718:727	arg1	display					695:701	the dense display	685:701	the dense display of glycans and morphology of mucin	685:736	To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin.					
34056092	0	31	theme	Mucin	30:34	arg1	Polymers					44:51	Mucin Mimetic Polymers	30:51	Mucin Mimetic Polymers	30:51	Stereochemical Control Yields Mucin Mimetic Polymers.					
34056092	7	32	contain	containing	870:879	arg2	cis-					888:891	cis-	888:891	cis-	888:891	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	7	32	contain	containing	870:879	arg2	trans-alkenes					896:908	trans-alkenes	896:908	trans-alkenes	896:908	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	7	32	contain	containing	870:879	arg1	glycopolymers					856:868	substituted norbornene-derived glycopolymers	825:868	substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes	825:908	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	5	33	theme	extended	503:510	arg1	conformation					512:523	the extended conformation	499:523	the extended conformation of mucins	499:533	We postulated that the extended conformation of mucins is critical for their ability to block microbial virulence phenotypes.					
34056092	5	33	theme	extended	503:510	arg1	critical					538:545	critical	538:545	critical	538:545	We postulated that the extended conformation of mucins is critical for their ability to block microbial virulence phenotypes.					
34056092	8	34	theme	allylic	960:966	arg1	strain					968:973	allylic strain	960:973	allylic strain	960:973	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	7	35	theme	substituted	825:835	arg1	glycopolymers					856:868	substituted norbornene-derived glycopolymers	825:868	substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes	825:908	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	3	36	theme	molecular	265:273	arg1	O-glycoproteins					282:296	mucins-high molecular weight O-glycoproteins	253:296	mucins-high molecular weight O-glycoproteins that adopt extended linear structures	253:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	3	36	theme	molecular	265:273	arg1	components					219:228	The primary components	207:228	The primary components of mucus hydrogels	207:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	8	37	dep	cis-	990:993	arg1	norbornene					1018:1027	norbornene	1018:1027	norbornene	1018:1027	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	0	38	theme	Mimetic	36:42	arg1	Polymers					44:51	Mucin Mimetic Polymers	30:51	Mucin Mimetic Polymers	30:51	Stereochemical Control Yields Mucin Mimetic Polymers.					
34056092	12	39	theme	backbone	1563:1570	arg1	importance					1537:1546	the importance	1533:1546	the importance of the polymer backbone in mucin surrogate design	1533:1596	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	3	40	theme	weight	275:280	arg1	O-glycoproteins					282:296	mucins-high molecular weight O-glycoproteins	253:296	mucins-high molecular weight O-glycoproteins that adopt extended linear structures	253:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	3	40	theme	weight	275:280	arg1	components					219:228	The primary components	207:228	The primary components of mucus hydrogels	207:247	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	4	41	theme	mucin	366:370	arg1	function					372:379	mucin function	366:379	mucin function	366:379	Glycosylation is integral to mucin function, but other characteristics that give rise to their advantageous biological activities are unknown.					
34056092	11	42	theme	potent	1447:1452	arg1	binders					1454:1460	more potent binders	1442:1460	more potent binders of a bacterial virulence factor, cholera toxin	1442:1507	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	9	43	dep	extended	1236:1243	arg1	mucin-like					1246:1255	mucin-like	1246:1255	mucin-like	1246:1255	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	12	44	theme	surrogate	1581:1589	arg1	design					1591:1596	mucin surrogate design	1575:1596	mucin surrogate design	1575:1596	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	7	45	from	catalyst	753:760	arg1	ROMP					807:810	ROMP	807:810	ROMP	807:810	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	7	45	from	catalyst	753:760	arg1	polymerization					791:804	a ring-opening metathesis polymerization	765:804	a ring-opening metathesis polymerization (ROMP)	765:811	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	5	46	theme	microbial	574:582	arg1	phenotypes					594:603	microbial virulence phenotypes	574:603	microbial virulence phenotypes	574:603	We postulated that the extended conformation of mucins is critical for their ability to block microbial virulence phenotypes.					
34056092	12	47	theme	polymer	1555:1561	arg1	backbone					1563:1570	the polymer backbone	1551:1570	the polymer backbone	1551:1570	Our findings highlight the importance of the polymer backbone in mucin surrogate design and underscore the significance of the extended mucin backbone for inhibiting virulence.					
34056092	4	48	theme	biological	445:454	arg1	activities					456:465	their advantageous biological activities	426:465	their advantageous biological activities	426:465	Glycosylation is integral to mucin function, but other characteristics that give rise to their advantageous biological activities are unknown.					
34056092	5	49	theme	virulence	584:592	arg1	phenotypes					594:603	microbial virulence phenotypes	574:603	microbial virulence phenotypes	574:603	We postulated that the extended conformation of mucins is critical for their ability to block microbial virulence phenotypes.					
34056092	9	50	theme	extended	1236:1243	arg1	structures					1257:1266	extended, mucin-like structures	1236:1266	extended, mucin-like structures	1236:1266	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	9	51	theme	polymers	1141:1148	arg1	micrographs					1122:1132	High-resolution atomic force micrographs	1093:1132	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins	1093:1201	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	4	52	theme	advantageous	432:443	arg1	activities					456:465	their advantageous biological activities	426:465	their advantageous biological activities	426:465	Glycosylation is integral to mucin function, but other characteristics that give rise to their advantageous biological activities are unknown.					
34056092	6	53	theme	dense	689:693	arg1	display					695:701	the dense display	685:701	the dense display of glycans and morphology of mucin	685:736	To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin.					
34056092	7	54	theme	metathesis	780:789	arg1	ROMP					807:810	ROMP	807:810	ROMP	807:810	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	7	54	theme	metathesis	780:789	arg1	polymerization					791:804	a ring-opening metathesis polymerization	765:804	a ring-opening metathesis polymerization (ROMP)	765:811	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	3	55	theme	extended	309:316	arg1	structures					325:334	extended linear structures	309:334	extended linear structures	309:334	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
34056092	8	56	theme	cis-	990:993	arg1	glycopolymers					1030:1042	cis- rather than trans-poly(norbornene) glycopolymers	990:1042	cis- rather than trans-poly(norbornene) glycopolymers	990:1042	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	9	57	theme	atomic	1109:1114	arg1	micrographs					1122:1132	High-resolution atomic force micrographs	1093:1132	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins	1093:1201	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	9	58	theme	purified	1163:1170	arg1	Muc2					1172:1175	Muc2	1172:1175	Muc2	1172:1175	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	11	59	theme	factor	1487:1492	arg1	binders					1454:1460	more potent binders	1442:1460	more potent binders of a bacterial virulence factor, cholera toxin	1442:1507	Consistent with mucin's linear morphology, cis-glycopolymers were more potent binders of a bacterial virulence factor, cholera toxin.					
34056092	8	60	theme	Conformational	911:924	arg1	analysis					926:933	Conformational analysis	911:933	Conformational analysis of the polymers based on allylic strain	911:973	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	8	61	theme	trans-poly	1007:1016	arg1	glycopolymers					1030:1042	cis- rather than trans-poly(norbornene) glycopolymers	990:1042	cis- rather than trans-poly(norbornene) glycopolymers	990:1042	Conformational analysis of the polymers based on allylic strain suggested that cis- rather than trans-poly(norbornene) glycopolymers would adopt linear structures that mimic mucins.					
34056092	9	62	theme	force	1116:1120	arg1	micrographs					1122:1132	High-resolution atomic force micrographs	1093:1132	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins	1093:1201	High-resolution atomic force micrographs of our polymers and natively purified Muc2, Muc5AC, and Muc5B mucins revealed that cis-polymers adopt extended, mucin-like structures.					
34056092	7	63	theme	ring-opening	767:778	arg1	ROMP					807:810	ROMP	807:810	ROMP	807:810	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	7	63	theme	ring-opening	767:778	arg1	polymerization					791:804	a ring-opening metathesis polymerization	765:804	a ring-opening metathesis polymerization (ROMP)	765:811	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	7	64	link	norbornene-derived	837:854	arg1	glycopolymers					856:868	substituted norbornene-derived glycopolymers	825:868	substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes	825:908	We varied the catalyst in a ring-opening metathesis polymerization (ROMP) to generate substituted norbornene-derived glycopolymers containing either cis- or trans-alkenes.					
34056092	3	65	dep	extended	309:316	arg1	linear					318:323	linear	318:323	linear	318:323	The primary components of mucus hydrogels are mucins-high molecular weight O-glycoproteins that adopt extended linear structures.					
33379016	6	0	theme	optimized	1137:1145	arg1	time					1158:1161	the optimized incubation time	1133:1161	the optimized incubation time	1133:1161	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	1	from	1.0 × 10-1 ng mL-1	1322:1339	arg1	concentrations					1288:1301	concentrations	1288:1301	concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1	1288:1339	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	7	2	theme	proposed	1812:1819	arg1	immunosensor					1821:1832	the proposed immunosensor	1808:1832	the proposed immunosensor	1808:1832	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	6	3	theme	analytical	1230:1239	arg1	performances					1241:1252	excellent analytical performances	1220:1252	excellent analytical performances	1220:1252	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	3	theme	analytical	1230:1239	arg1	range					1279:1283	a wide linear range	1265:1283	a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1	1265:1339	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	1	4	theme	selective	228:236	arg1	immunosensor					265:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA).					
33379016	1	5	theme	electrochemical	249:263	arg1	immunosensor					265:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA).					
33379016	7	6	theme	human	1671:1675	arg1	serum					1677:1681	human serum	1671:1681	human serum	1671:1681	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	2	7	theme	gold	584:587	arg1	AuNPs					604:608	AuNPs	604:608	AuNPs	604:608	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	7	theme	gold	584:587	arg1	nanoparticles					589:601	gold nanoparticles	584:601	gold nanoparticles (AuNPs)	584:609	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	7	8	theme	promising	1785:1793	arg1	potential					1795:1803	the promising potential	1781:1803	the promising potential of the proposed immunosensor in clinical diagnosis	1781:1854	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	6	9	theme	developed	1187:1195	arg1	immunosensor					1197:1208	the developed immunosensor	1183:1208	the developed immunosensor	1183:1208	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	10	theme	good	1403:1406	arg1	reproducibility					1408:1422	good reproducibility	1403:1422	good reproducibility	1403:1422	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	5	11	theme	PSA/anti-PSA	1099:1110	arg1	immunocomplex					1112:1124	PSA/anti-PSA immunocomplex	1099:1124	PSA/anti-PSA immunocomplex	1099:1124	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	7	12	theme	PSA	1664:1666	arg1	determination					1647:1659	the determination	1643:1659	the determination of PSA	1643:1666	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	0	13	theme	prostate-specific	178:194	arg1	antigen					196:202	prostate-specific antigen	178:202	prostate-specific antigen	178:202	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	6	14	theme	90	1482:1483	arg1	%					1484:1484	%	1484:1484	%	1484:1484	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	4	15	theme	current	930:936	arg1	signal					938:943	the current signal	926:943	the current signal	926:943	The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal.					
33379016	6	16	theme	detection	1367:1375	arg1	limit					1358:1362	a very low limit	1347:1362	a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1347:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	5	17	theme	pulse	959:963	arg1	voltammetry					965:975	Differential pulse voltammetry	946:975	Differential pulse voltammetry	946:975	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	6	18	theme	linear	1272:1277	arg1	range					1279:1283	a wide linear range	1265:1283	a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1	1265:1339	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	3	19	theme	cryogel	653:659	arg1	biocompatibility					616:631	biocompatibility	616:631	biocompatibility	616:631	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	3	19	theme	cryogel	653:659	arg1	porosity					637:644	porosity	637:644	porosity	637:644	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	6	20	from	concentrations	1288:1301	arg1	range					1279:1283	a wide linear range	1265:1283	a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1	1265:1339	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	21	theme	%	1460:1460	arg1	n = 6					1463:1467	n = 6	1463:1467	n = 6	1463:1467	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	21	theme	%	1460:1460	arg1	deviation					1443:1451	relative standard deviation	1425:1451	relative standard deviation of <4.6%	1425:1460	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	22	theme	selectivity	1589:1599	arg1	detection					1367:1375	detection	1367:1375	detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1367:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	2	23	theme	porous	526:531	arg1	cryogel					533:539	3D porous cryogel	523:539	3D porous cryogel (CS-GR-IL-Fc cry)	523:557	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	23	theme	porous	526:531	arg1	cry					554:556	CS-GR-IL-Fc cry	542:556	CS-GR-IL-Fc cry	542:556	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	0	24	theme	liquid-ferrocene	101:116	arg1	nanocomposite					118:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	1	25	theme	sensitive	214:222	arg1	immunosensor					265:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA).					
33379016	7	26	theme	immunosorbent	1735:1747	arg1	P > 0.05					1756:1763	P > 0.05	1756:1763	P > 0.05	1756:1763	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	7	26	theme	immunosorbent	1735:1747	arg1	assay					1749:1753	the enzyme-linked immunosorbent assay	1717:1753	the enzyme-linked immunosorbent assay (P > 0.05)	1717:1764	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	6	27	theme	standard	1434:1441	arg1	n = 6					1463:1467	n = 6	1463:1467	n = 6	1463:1467	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	27	theme	standard	1434:1441	arg1	deviation					1443:1451	relative standard deviation	1425:1451	relative standard deviation of <4.6%	1425:1460	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	2	28	theme	screen-printed	452:465	arg1	electrode					474:482	a screen-printed carbon electrode	450:482	a screen-printed carbon electrode (SPCE)	450:489	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	28	theme	screen-printed	452:465	arg1	SPCE					485:488	SPCE	485:488	SPCE	485:488	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	0	29	dep	cryogel	132:138	arg1	decorated					140:148	decorated	140:148	cryogel decorated with gold nanoparticles for prostate-specific antigen	132:202	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	6	30	theme	%	1484:1484	arg1	sensitivity					1486:1496	90% sensitivity	1482:1496	90% sensitivity within 20 days	1482:1511	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	0	31	theme	label-free	18:27	arg1	immunosensor					45:56	An ultrasensitive label-free electrochemical immunosensor	0:56	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	0:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	4	32	theme	CS-GR-IL-Fc	833:843	arg1	cry					845:847	The CS-GR-IL-Fc cry	829:847	The CS-GR-IL-Fc cry	829:847	The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal.					
33379016	0	33	theme	porous	70:75	arg1	nanocomposite					118:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	6	34	dep	repeatability	1515:1527	arg1	cycles					1533:1538	12 cycles	1530:1538	12 cycles of binding-rebinding	1530:1559	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	34	dep	repeatability	1515:1527	arg1	%					1582:1582	the sensitivity > 90%	1562:1582	the sensitivity > 90%	1562:1582	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	1	35	theme	prostate-specific	320:336	arg1	PSA					347:349	PSA	347:349	PSA	347:349	A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA).					
33379016	1	35	theme	prostate-specific	320:336	arg1	antigen					338:344	prostate-specific antigen	320:344	prostate-specific antigen (PSA)	320:350	A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA).					
33379016	5	36	theme	oxidation	1044:1052	arg1	current					1059:1065	the Fc oxidation peak current	1037:1065	the Fc oxidation peak current	1037:1065	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	7	37	theme	clinical	1837:1844	arg1	diagnosis					1846:1854	clinical diagnosis	1837:1854	clinical diagnosis	1837:1854	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	6	38	theme	incubation	1147:1156	arg1	time					1158:1161	the optimized incubation time	1133:1161	the optimized incubation time	1133:1161	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	39	theme	low	1354:1356	arg1	limit					1358:1362	a very low limit	1347:1362	a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1347:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	5	40	from	reduction	1024:1032	arg1	current					1059:1065	the Fc oxidation peak current	1037:1065	the Fc oxidation peak current	1037:1065	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	5	40	from	reduction	1024:1032	arg1	response					1070:1077	response	1070:1077	response to the formation of PSA/anti-PSA immunocomplex	1070:1124	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	6	41	dep	1.0 × 10-1 ng mL-1	1322:1339	arg1	to					1319:1320	to	1319:1320	to	1319:1320	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	4	42	theme	electron	893:900	arg1	transfer					902:909	electron transfer	893:909	electron transfer	893:909	The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal.					
33379016	7	43	theme	immunosensor	1821:1832	arg1	potential					1795:1803	the promising potential	1781:1803	the promising potential of the proposed immunosensor in clinical diagnosis	1781:1854	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	3	44	theme	amino	720:724	arg1	groups					726:731	amino groups	720:731	amino groups	720:731	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	2	45	theme	chitosan	368:375	arg1	ferrocene					405:413	ferrocene	405:413	ferrocene (CS-GR-IL-Fc)	405:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	45	theme	chitosan	368:375	arg1	graphene					378:385	graphene	378:385	graphene	378:385	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	45	theme	chitosan	368:375	arg1	liquid					394:399	liquid	394:399	liquid	394:399	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	45	theme	chitosan	368:375	arg1	chitosan					368:375	chitosan	368:375	chitosan	368:375	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	45	theme	chitosan	368:375	arg1	composite					355:363	A composite	353:363	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc)	353:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	7	46	from	assay	1749:1753	arg1	results					1704:1710	results	1704:1710	results from the enzyme-linked immunosorbent assay (P > 0.05)	1704:1764	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	3	47	theme	anti-PSA	755:762	arg1	population					741:750	the population	737:750	the population	737:750	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	1	48	theme	label-free	238:247	arg1	immunosensor					265:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor	205:276	A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA).					
33379016	4	49	theme	excellent	859:867	arg1	conductivity					869:880	excellent conductivity	859:880	excellent conductivity	859:880	The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal.					
33379016	6	50	theme	excellent	1220:1228	arg1	performances					1241:1252	excellent analytical performances	1220:1252	excellent analytical performances	1220:1252	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	50	theme	excellent	1220:1228	arg1	range					1279:1283	a wide linear range	1265:1283	a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1	1265:1339	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	3	51	dep	biocompatibility	616:631	arg1	The					612:614	The	612:614	The	612:614	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	6	52	theme	reproducibility	1408:1422	arg1	detection					1367:1375	detection	1367:1375	detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1367:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	2	53	theme	graphene	378:385	arg1	ferrocene					405:413	ferrocene	405:413	ferrocene (CS-GR-IL-Fc)	405:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	53	theme	graphene	378:385	arg1	graphene					378:385	graphene	378:385	graphene	378:385	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	53	theme	graphene	378:385	arg1	liquid					394:399	liquid	394:399	liquid	394:399	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	53	theme	graphene	378:385	arg1	chitosan					368:375	chitosan	368:375	chitosan	368:375	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	53	theme	graphene	378:385	arg1	composite					355:363	A composite	353:363	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc)	353:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	6	54	theme	binding-rebinding	1543:1559	arg1	cycles					1533:1538	12 cycles	1530:1538	12 cycles of binding-rebinding	1530:1559	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	54	theme	binding-rebinding	1543:1559	arg1	%					1582:1582	the sensitivity > 90%	1562:1582	the sensitivity > 90%	1562:1582	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	4	55	dep	displayed	849:857	arg1	enhancing					883:891	enhancing	883:891	enhancing electron transfer	883:909	The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal.					
33379016	4	55	dep	displayed	849:857	arg1	amplifying					915:924	amplifying	915:924	amplifying the current signal	915:943	The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal.					
33379016	5	56	theme	immunocomplex	1112:1124	arg1	formation					1086:1094	the formation	1082:1094	the formation of PSA/anti-PSA immunocomplex	1082:1124	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	6	57	dep	4.8 × 10-8 ng mL-1	1380:1397	arg1	n = 6					1463:1467	n = 6	1463:1467	n = 6	1463:1467	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	57	dep	4.8 × 10-8 ng mL-1	1380:1397	arg1	deviation					1443:1451	relative standard deviation	1425:1451	relative standard deviation of <4.6%	1425:1460	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	7	58	with	consistent	1688:1697	arg1	results					1704:1710	results	1704:1710	results from the enzyme-linked immunosorbent assay (P > 0.05)	1704:1764	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	5	59	theme	Differential	946:957	arg1	voltammetry					965:975	Differential pulse voltammetry	946:975	Differential pulse voltammetry	946:975	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	6	60	theme	wide	1267:1270	arg1	range					1279:1283	a wide linear range	1265:1283	a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1	1265:1339	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	7	61	from	potential	1795:1803	arg1	diagnosis					1846:1854	clinical diagnosis	1837:1854	clinical diagnosis	1837:1854	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	0	62	theme	chitosan-graphene-ionic	77:99	arg1	nanocomposite					118:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	2	63	theme	cryogel	533:539	arg1	layer					514:518	a layer	512:518	a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs)	512:609	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	7	64	from	device	1632:1637	arg1	serum					1677:1681	human serum	1671:1681	human serum	1671:1681	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	2	65	theme	3D	523:524	arg1	cryogel					533:539	3D porous cryogel	523:539	3D porous cryogel (CS-GR-IL-Fc cry)	523:557	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	65	theme	3D	523:524	arg1	cry					554:556	CS-GR-IL-Fc cry	542:556	CS-GR-IL-Fc cry	542:556	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	6	66	theme	relative	1425:1432	arg1	n = 6					1463:1467	n = 6	1463:1467	n = 6	1463:1467	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	66	theme	relative	1425:1432	arg1	deviation					1443:1451	relative standard deviation	1425:1451	relative standard deviation of <4.6%	1425:1460	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	7	67	theme	enzyme-linked	1721:1733	arg1	P > 0.05					1756:1763	P > 0.05	1756:1763	P > 0.05	1756:1763	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	7	67	theme	enzyme-linked	1721:1733	arg1	assay					1749:1753	the enzyme-linked immunosorbent assay	1717:1753	the enzyme-linked immunosorbent assay (P > 0.05)	1717:1764	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	2	68	theme	CS-GR-IL-Fc	542:552	arg1	cryogel					533:539	3D porous cryogel	523:539	3D porous cryogel (CS-GR-IL-Fc cry)	523:557	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	68	theme	CS-GR-IL-Fc	542:552	arg1	cry					554:556	CS-GR-IL-Fc cry	542:556	CS-GR-IL-Fc cry	542:556	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	0	69	theme	gold	155:158	arg1	nanoparticles					160:172	gold nanoparticles	155:172	gold nanoparticles for prostate-specific antigen	155:202	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	0	70	theme	ultrasensitive	3:16	arg1	immunosensor					45:56	An ultrasensitive label-free electrochemical immunosensor	0:56	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	0:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	7	71	link	enzyme-linked	1721:1733	arg1	P > 0.05					1756:1763	P > 0.05	1756:1763	P > 0.05	1756:1763	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	7	71	link	enzyme-linked	1721:1733	arg1	assay					1749:1753	the enzyme-linked immunosorbent assay	1717:1753	the enzyme-linked immunosorbent assay (P > 0.05)	1717:1764	The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.					
33379016	0	72	theme	electrochemical	29:43	arg1	immunosensor					45:56	An ultrasensitive label-free electrochemical immunosensor	0:56	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	0:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	3	73	theme	surface	675:681	arg1	area					683:686	the surface area	671:686	the surface area available for AuNPs loading via amino groups	671:731	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	6	74	theme	stability	1471:1479	arg1	detection					1367:1375	detection	1367:1375	detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1367:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	6	75	dep	stability	1471:1479	arg1	sensitivity					1486:1496	90% sensitivity	1482:1496	90% sensitivity within 20 days	1482:1511	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	5	76	theme	peak	1054:1057	arg1	current					1059:1065	the Fc oxidation peak current	1037:1065	the Fc oxidation peak current	1037:1065	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
33379016	2	77	theme	carbon	467:472	arg1	electrode					474:482	a screen-printed carbon electrode	450:482	a screen-printed carbon electrode (SPCE)	450:489	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	77	theme	carbon	467:472	arg1	SPCE					485:488	SPCE	485:488	SPCE	485:488	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	0	78	theme	3D	67:68	arg1	nanocomposite					118:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite	67:130	An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan-graphene-ionic liquid-ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen.					
33379016	6	79	theme	4.8 × 10-8 ng mL-1	1380:1397	arg1	detection					1367:1375	detection	1367:1375	detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1367:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	3	80	theme	AuNPs	702:706	arg1	loading					708:714	AuNPs loading	702:714	AuNPs loading via amino groups	702:731	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	2	81	theme	ferrocene	405:413	arg1	ferrocene					405:413	ferrocene	405:413	ferrocene (CS-GR-IL-Fc)	405:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	81	theme	ferrocene	405:413	arg1	graphene					378:385	graphene	378:385	graphene	378:385	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	81	theme	ferrocene	405:413	arg1	liquid					394:399	liquid	394:399	liquid	394:399	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	81	theme	ferrocene	405:413	arg1	chitosan					368:375	chitosan	368:375	chitosan	368:375	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	81	theme	ferrocene	405:413	arg1	composite					355:363	A composite	353:363	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc)	353:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	6	82	theme	electrolyte	1167:1177	arg1	pH					1179:1180	electrolyte pH	1167:1180	electrolyte pH	1167:1180	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	3	83	theme	available	688:696	arg1	area					683:686	the surface area	671:686	the surface area available for AuNPs loading via amino groups	671:731	The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased.					
33379016	2	84	dep	casted	438:443	arg1	drop					433:436	drop	433:436	drop	433:436	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	85	theme	liquid	394:399	arg1	ferrocene					405:413	ferrocene	405:413	ferrocene (CS-GR-IL-Fc)	405:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	85	theme	liquid	394:399	arg1	graphene					378:385	graphene	378:385	graphene	378:385	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	85	theme	liquid	394:399	arg1	liquid					394:399	liquid	394:399	liquid	394:399	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	85	theme	liquid	394:399	arg1	chitosan					368:375	chitosan	368:375	chitosan	368:375	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	2	85	theme	liquid	394:399	arg1	composite					355:363	A composite	353:363	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc)	353:427	A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR-IL-Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs).					
33379016	6	86	theme	repeatability	1515:1527	arg1	detection					1367:1375	detection	1367:1375	detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity	1367:1599	Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10-7 to 1.0 × 10-1 ng mL-1, with a very low limit of detection of 4.8 × 10-8 ng mL-1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding-rebinding, the sensitivity > 90%) and selectivity.					
33379016	5	87	theme	Fc	1041:1042	arg1	current					1059:1065	the Fc oxidation peak current	1037:1065	the Fc oxidation peak current	1037:1065	Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex.					
32720840	0	0	theme	assisted	82:89	arg1	process					91:97	oxalate oxidase assisted process	66:97	oxalate oxidase assisted process	66:97	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	2	1	theme	various	442:448	arg1	applications					455:466	various food applications	442:466	various food applications	442:466	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	9	2	theme	optimization	1351:1362	arg1	This					1323:1326	This	1323:1326	This	1323:1326	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	9	2	theme	optimization	1351:1362	arg1	report					1341:1346	the first report	1331:1346	the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase	1331:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	2	3	theme	amount	324:329	arg1	presence					300:307	the presence	296:307	the presence of significant amount of calcium oxalate crystals	296:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	0	4	from	flour	57:61	arg1	Optimization					0:11	Optimization	0:11	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.	0:98	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	0	4	from	flour	57:61	arg1	production					36:45	oxalate-free starch production	16:45	oxalate-free starch production from Taro flour	16:61	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	9	5	theme	production	1371:1380	arg1	optimization					1351:1362	optimization	1351:1362	optimization of the production of starch from taro flour using microbial oxalate oxidase	1351:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	0	6	theme	oxidase	74:80	arg1	process					91:97	oxalate oxidase assisted process	66:97	oxalate oxidase assisted process	66:97	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	7	7	theme	taro	1111:1114	arg1	concentration					1122:1134	taro flour concentration	1111:1134	taro flour concentration	1111:1134	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	9	8	theme	microbial	1414:1422	arg1	oxidase					1432:1438	microbial oxalate oxidase	1414:1438	microbial oxalate oxidase	1414:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	2	9	theme	significant	312:322	arg1	amount					324:329	significant amount	312:329	significant amount of calcium oxalate crystals	312:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	2	9	theme	significant	312:322	arg1	crystals					350:357	calcium oxalate crystals	334:357	calcium oxalate crystals	334:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	8	10	theme	model	1209:1213	arg1	Validity					1193:1200	Validity	1193:1200	Validity of the model	1193:1213	Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions.					
32720840	7	11	theme	oxalate	1176:1182	arg1	removal					1184:1190	the oxalate removal	1172:1190	the oxalate removal	1172:1190	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	0	12	from	Optimization	0:11	arg1	flour					57:61	Taro flour	52:61	Taro flour	52:61	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	6	13	theme	central	890:896	arg1	design					908:913	A central composite design	888:913	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point	888:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	4	14	theme	starch	738:743	arg1	properties					724:733	the unique properties	713:733	the unique properties of starch	713:743	However, using oxalate oxidase can effectively remove oxalates without altering the unique properties of starch.					
32720840	1	15	theme	small	245:249	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	15	theme	small	245:249	arg1	granules					251:258	small granules	245:258	small granules	245:258	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	16	contain	possess	146:152	arg1	starch					127:132	Taro (Colocasia esculenta) starch	100:132	Taro (Colocasia esculenta) starch	100:132	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	16	contain	possess	146:152	arg2	properties					185:194	unique physical and functional properties	154:194	unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc	154:286	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	16	contain	possess	146:152	arg2	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	8	17	theme	optimal	1303:1309	arg1	conditions					1311:1320	optimal conditions	1303:1320	optimal conditions	1303:1320	Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions.					
32720840	3	18	theme	chemical	522:529	arg1	methods					531:537	various physical and chemical methods	501:537	various physical and chemical methods	501:537	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	6	19	dep	four	1005:1008	arg1	replicates					1010:1019	replicates	1010:1019	replicates at the center point	1010:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	3	20	theme	various	501:507	arg1	methods					531:537	various physical and chemical methods	501:537	various physical and chemical methods	501:537	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	5	21	theme	response	858:865	arg1	methodology					875:885	response surface methodology	858:885	response surface methodology	858:885	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	1	22	theme	unique	154:159	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	22	theme	unique	154:159	arg1	properties					185:194	unique physical and functional properties	154:194	unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc	154:286	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	7	23	theme	concentration	1122:1134	arg1	effect					1101:1106	the effect	1097:1106	the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal	1097:1190	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	3	24	theme	physical	509:516	arg1	methods					531:537	various physical and chemical methods	501:537	various physical and chemical methods	501:537	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	1	25	theme	physical	161:168	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	25	theme	physical	161:168	arg1	properties					185:194	unique physical and functional properties	154:194	unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc	154:286	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	2	26	theme	unique	407:412	arg1	source					431:436	this unique and cheap starch source	402:436	this unique and cheap starch source for various food applications	402:466	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	6	27	with	trials	942:947	arg1	points					962:967	10 cube points	954:967	10 cube points augmented with six axial points and four replicates at the center point	954:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	7	28	theme	incubation	1153:1162	arg1	time					1164:1167	incubation time	1153:1167	incubation time	1153:1167	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	2	29	theme	crystals	350:357	arg1	amount					324:329	significant amount	312:329	significant amount of calcium oxalate crystals	312:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	2	29	theme	crystals	350:357	arg1	crystals					350:357	calcium oxalate crystals	334:357	calcium oxalate crystals	334:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	5	30	theme	oxidase	793:799	arg1	process					828:834	oxalate oxidase assisted starch extraction process	785:834	oxalate oxidase assisted starch extraction process	785:834	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	7	31	theme	enzyme	1137:1142	arg1	load					1144:1147	enzyme load	1137:1147	enzyme load	1137:1147	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	9	32	theme	starch	1385:1390	arg1	production					1371:1380	the production	1367:1380	the production of starch from taro flour using microbial oxalate oxidase	1367:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	3	33	theme	oxalate	549:555	arg1	content					557:563	oxalate content	549:563	oxalate content of starch	549:573	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	1	34	theme	functional	174:183	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	34	theme	functional	174:183	arg1	properties					185:194	unique physical and functional properties	154:194	unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc	154:286	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	0	35	theme	starch	29:34	arg1	production					36:45	oxalate-free starch production	16:45	oxalate-free starch production from Taro flour	16:61	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	1	36	theme	higher	261:266	arg1	power					277:281	higher swelling power	261:281	higher swelling power	261:281	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	36	theme	higher	261:266	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	7	37	theme	mathematical	1056:1067	arg1	model					1069:1073	A mathematical model	1054:1073	A mathematical model	1054:1073	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	9	38	theme	taro	1397:1400	arg1	flour					1402:1406	taro flour	1397:1406	taro flour using microbial oxalate oxidase	1397:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	1	39	theme	Taro	100:103	arg1	starch					127:132	Taro (Colocasia esculenta) starch	100:132	Taro (Colocasia esculenta) starch	100:132	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	5	40	theme	extraction	817:826	arg1	process					828:834	oxalate oxidase assisted starch extraction process	785:834	oxalate oxidase assisted starch extraction process	785:834	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	0	41	theme	oxalate-free	16:27	arg1	production					36:45	oxalate-free starch production	16:45	oxalate-free starch production from Taro flour	16:61	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	1	42	theme	swelling	268:275	arg1	power					277:281	higher swelling power	261:281	higher swelling power	261:281	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	42	theme	swelling	268:275	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	3	43	theme	Traditional	469:479	arg1	processes					481:489	Traditional processes	469:489	Traditional processes	469:489	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	6	44	theme	cube	957:960	arg1	points					962:967	10 cube points	954:967	10 cube points augmented with six axial points and four replicates at the center point	954:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	7	45	theme	time	1164:1167	arg1	effect					1101:1106	the effect	1097:1106	the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal	1097:1190	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	3	46	theme	starch	568:573	arg1	content					557:563	oxalate content	549:563	oxalate content of starch	549:573	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	2	47	theme	oxalate	342:348	arg1	crystals					350:357	calcium oxalate crystals	334:357	calcium oxalate crystals	334:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	7	48	theme	flour	1116:1120	arg1	concentration					1122:1134	taro flour concentration	1111:1134	taro flour concentration	1111:1134	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	2	49	theme	starch	424:429	arg1	source					431:436	this unique and cheap starch source	402:436	this unique and cheap starch source for various food applications	402:466	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	0	50	theme	production	36:45	arg1	Optimization					0:11	Optimization	0:11	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.	0:98	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	3	51	theme	functional	610:619	arg1	properties					621:630	its physical and functional properties	593:630	its physical and functional properties	593:630	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	5	52	theme	assisted	801:808	arg1	process					828:834	oxalate oxidase assisted starch extraction process	785:834	oxalate oxidase assisted starch extraction process	785:834	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	5	53	theme	taro	841:844	arg1	flour					846:850	taro flour	841:850	taro flour using response surface methodology	841:885	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	1	54	theme	low	204:206	arg1	etc					284:286	etc	284:286	etc	284:286	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	54	theme	low	204:206	arg1	power					277:281	higher swelling power	261:281	higher swelling power	261:281	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	54	theme	low	204:206	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	54	theme	low	204:206	arg1	form					239:242	A-crystalline form	225:242	A-crystalline form	225:242	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	54	theme	low	204:206	arg1	granules					251:258	small granules	245:258	small granules	245:258	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	2	55	theme	cheap	418:422	arg1	source					431:436	this unique and cheap starch source	402:436	this unique and cheap starch source for various food applications	402:466	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	7	56	from	effect	1101:1106	arg1	removal					1184:1190	the oxalate removal	1172:1190	the oxalate removal	1172:1190	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	6	57	theme	center	1028:1033	arg1	point					1035:1039	the center point	1024:1039	the center point	1024:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	1	58	theme	Colocasia	106:114	arg1	Taro					100:103	Taro	100:103	Taro (Colocasia esculenta) starch	100:132	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	58	theme	Colocasia	106:114	arg1	esculenta					116:124	Colocasia esculenta	106:124	Colocasia esculenta	106:124	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	0	59	theme	Taro	52:55	arg1	flour					57:61	Taro flour	52:61	Taro flour	52:61	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	9	60	theme	first	1335:1339	arg1	This					1323:1326	This	1323:1326	This	1323:1326	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	9	60	theme	first	1335:1339	arg1	report					1341:1346	the first report	1331:1346	the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase	1331:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	6	61	theme	axial	988:992	arg1	points					994:999	six axial points	984:999	six axial points	984:999	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	9	62	from	flour	1402:1406	arg1	optimization					1351:1362	optimization	1351:1362	optimization of the production of starch from taro flour using microbial oxalate oxidase	1351:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	9	62	from	flour	1402:1406	arg1	production					1371:1380	the production	1367:1380	the production of starch from taro flour using microbial oxalate oxidase	1367:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	4	63	theme	oxalate	648:654	arg1	oxidase					656:662	oxalate oxidase	648:662	oxalate oxidase	648:662	However, using oxalate oxidase can effectively remove oxalates without altering the unique properties of starch.					
32720840	6	64	theme	experimental	929:940	arg1	trials					942:947	20 experimental trials	926:947	20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point	926:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	3	65	theme	physical	597:604	arg1	properties					621:630	its physical and functional properties	593:630	its physical and functional properties	593:630	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	3	66	dep	remove	542:547	arg1	change					586:591	change	586:591	change its physical and functional properties	586:630	Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties.					
32720840	5	67	theme	surface	867:873	arg1	methodology					875:885	response surface methodology	858:885	response surface methodology	858:885	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	1	68	theme	amylose	208:214	arg1	etc					284:286	etc	284:286	etc	284:286	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	68	theme	amylose	208:214	arg1	power					277:281	higher swelling power	261:281	higher swelling power	261:281	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	68	theme	amylose	208:214	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	68	theme	amylose	208:214	arg1	form					239:242	A-crystalline form	225:242	A-crystalline form	225:242	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	68	theme	amylose	208:214	arg1	granules					251:258	small granules	245:258	small granules	245:258	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	9	69	from	optimization	1351:1362	arg1	flour					1402:1406	taro flour	1397:1406	taro flour using microbial oxalate oxidase	1397:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	8	70	theme	total	1267:1271	arg1	oxalates					1273:1280	total oxalates	1267:1280	total oxalates	1267:1280	Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions.					
32720840	7	71	theme	load	1144:1147	arg1	effect					1101:1106	the effect	1097:1106	the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal	1097:1190	A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal.					
32720840	4	72	theme	unique	717:722	arg1	properties					724:733	the unique properties	713:733	the unique properties of starch	713:743	However, using oxalate oxidase can effectively remove oxalates without altering the unique properties of starch.					
32720840	0	73	theme	oxalate	66:72	arg1	process					91:97	oxalate oxidase assisted process	66:97	oxalate oxidase assisted process	66:97	Optimization of oxalate-free starch production from Taro flour by oxalate oxidase assisted process.					
32720840	2	74	theme	food	364:367	arg1	industry					369:376	the food industry	360:376	the food industry	360:376	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	2	74	theme	food	364:367	arg1	reluctant					381:389	reluctant	381:389	reluctant	381:389	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	9	75	theme	oxalate	1424:1430	arg1	oxidase					1432:1438	microbial oxalate oxidase	1414:1438	microbial oxalate oxidase	1414:1438	This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase.					
32720840	2	76	theme	calcium	334:340	arg1	crystals					350:357	calcium oxalate crystals	334:357	calcium oxalate crystals	334:357	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	8	77	theme	oxalates	1273:1280	arg1	%					1262:1262	98.3%	1258:1262	98.3% of total oxalates	1258:1280	Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions.					
32720840	8	77	theme	oxalates	1273:1280	arg1	oxalates					1273:1280	total oxalates	1267:1280	total oxalates	1267:1280	Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions.					
32720840	6	78	theme	composite	898:906	arg1	design					908:913	A central composite design	888:913	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point	888:1039	A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied.					
32720840	2	79	theme	food	450:453	arg1	applications					455:466	various food applications	442:466	various food applications	442:466	Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications.					
32720840	5	80	theme	starch	810:815	arg1	process					828:834	oxalate oxidase assisted starch extraction process	785:834	oxalate oxidase assisted starch extraction process	785:834	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	5	81	theme	oxalate	785:791	arg1	process					828:834	oxalate oxidase assisted starch extraction process	785:834	oxalate oxidase assisted starch extraction process	785:834	Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology.					
32720840	1	82	theme	A-crystalline	225:237	arg1	content					216:222	low amylose content	204:222	low amylose content	204:222	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32720840	1	82	theme	A-crystalline	225:237	arg1	form					239:242	A-crystalline form	225:242	A-crystalline form	225:242	Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc.					
32279810	4	0	theme	mechanical	629:638	arg1	property					640:647	mechanical property	629:647	mechanical property of scaffolds	629:660	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	12	1	theme	nano-pearl	1927:1936	arg1	NPP/C-HA					1971:1978	NPP/C-HA	1971:1978	NPP/C-HA	1971:1978	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	12	1	theme	nano-pearl	1927:1936	arg1	acid					1965:1968	nano-pearl powder/chitosan-hyaluronic acid	1927:1968	the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds	1923:1989	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	2	2	theme	bioactive	314:322	arg1	characteristics					324:338	their bioactive characteristics	308:338	their bioactive characteristics	308:338	Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties.					
32279810	12	3	theme	acid	1965:1968	arg1	biomaterial					2016:2026	a promising biomaterial	2004:2026	a promising biomaterial for bone tissue engineering	2004:2054	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	12	3	theme	acid	1965:1968	arg1	scaffolds					1981:1989	the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds	1923:1989	the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds	1923:1989	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	10	4	with	scaffolds	1616:1624	arg1	proportion					1642:1651	higher NPP proportion	1631:1651	higher NPP proportion	1631:1651	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	6	5	theme	alkaline	1168:1175	arg1	phosphatase					1177:1187	alkaline phosphatase	1168:1187	alkaline phosphatase activity (ALP)	1168:1202	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	4	6	theme	mercury	714:720	arg1	porosimeter					722:732	mercury porosimeter	714:732	mercury porosimeter	714:732	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	2	7	theme	mechanical	354:363	arg1	properties					365:374	favorable mechanical properties	344:374	favorable mechanical properties	344:374	Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties.					
32279810	1	8	theme	bone	219:222	arg1	regeneration					224:235	bone regeneration	219:235	bone regeneration	219:235	Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration.					
32279810	4	9	theme	electron	687:694	arg1	microscopy					696:705	scanning electron microscopy	678:705	scanning electron microscopy (SEM)	678:711	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	4	9	theme	electron	687:694	arg1	SEM					708:710	SEM	708:710	SEM	708:710	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	6	10	theme	Western	1218:1224	arg1	blotting					1226:1233	Western blotting	1218:1233	Western blotting	1218:1233	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	8	11	dep	increased	1378:1386	arg1	whereas					1406:1412	whereas	1406:1412	whereas	1406:1412	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	11	12	theme	Runx2	1899:1903	arg1	genes					1905:1909	Runx2 genes	1899:1909	Runx2 genes	1899:1909	The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes.					
32279810	5	13	theme	X-ray	877:881	arg1	XRD					896:898	XRD	896:898	XRD	896:898	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	5	13	theme	X-ray	877:881	arg1	diffraction					883:893	X-ray diffraction	877:893	X-ray diffraction (XRD)	877:899	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	11	14	theme	OPN	1891:1893	arg1	expression					1864:1873	the expression	1860:1873	the expression of Col αI, OCN, OPN and Runx2 genes	1860:1909	The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes.					
32279810	12	15	theme	bone	2032:2035	arg1	engineering					2044:2054	bone tissue engineering	2032:2054	bone tissue engineering	2032:2054	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	5	16	dep	infrared	984:991	arg1	FTIR					994:997	FTIR	994:997	FTIR	994:997	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	8	17	theme	%	1466:1466	arg1	scaffold					1468:1475	25% scaffold	1464:1475	25% scaffold	1464:1475	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	5	18	theme	crystal	811:817	arg1	form					819:822	The crystal form	807:822	The crystal form	807:822	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	10	19	theme	nano-pearl	1767:1776	arg1	powder					1778:1783	nano-pearl powder	1767:1783	nano-pearl powder	1767:1783	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	9	20	theme	high	1498:1501	arg1	porosity					1503:1510	a high porosity	1496:1510	a high porosity between 89% and 93%	1496:1530	The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP.					
32279810	10	21	dep	proliferation	1677:1689	arg1	the					1673:1675	the	1673:1675	the	1673:1675	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	1	22	theme	tissue	127:132	arg1	engineering					134:144	Bone tissue engineering	122:144	Bone tissue engineering using osteoinductive scaffolds	122:175	Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration.					
32279810	0	23	theme	mechanism	111:119	arg1	scaffold					60:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	0	23	theme	mechanism	111:119	arg1	study					85:89	preliminary study	73:89	preliminary study of its osteogenesis mechanism	73:119	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	10	24	theme	cells	1723:1727	arg1	proliferation					1677:1689	proliferation	1677:1689	proliferation	1677:1689	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	10	24	theme	cells	1723:1727	arg1	differentiation					1695:1709	differentiation	1695:1709	differentiation	1695:1709	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	4	25	theme	material	765:772	arg1	machine					785:791	universal material experiment machine	755:791	universal material experiment machine respectively	755:804	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	11	26	theme	OCN	1886:1888	arg1	expression					1864:1873	the expression	1860:1873	the expression of Col αI, OCN, OPN and Runx2 genes	1860:1909	The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes.					
32279810	8	27	theme	mechanical	1343:1352	arg1	properties					1354:1363	mechanical properties	1343:1363	mechanical properties	1343:1363	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	3	28	theme	nano-pearl	494:503	arg1	NPP					513:515	NPP	513:515	NPP	513:515	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	3	28	theme	nano-pearl	494:503	arg1	powder					505:510	nano-pearl powder	494:510	nano-pearl powder (NPP)	494:516	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	2	29	from	interest	271:278	arg1	regeneration					288:299	bone regeneration	283:299	bone regeneration	283:299	Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties.					
32279810	6	30	theme	related	1085:1091	arg1	expression					1098:1107	osteogenic related gene expression	1074:1107	osteogenic related gene expression	1074:1107	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	0	31	theme	Nano-pearl	0:9	arg1	scaffold					60:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	7	32	theme	C-HA	1307:1310	arg1	scaffolds					1312:1320	the C-HA scaffolds	1303:1320	the C-HA scaffolds	1303:1320	The results revealed that the NPP was successfully incorporated in the C-HA scaffolds.					
32279810	2	33	theme	bone	283:286	arg1	regeneration					288:299	bone regeneration	283:299	bone regeneration	283:299	Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties.					
32279810	9	34	contain	had	1492:1494	arg1	scaffolds					1482:1490	The scaffolds	1478:1490	The scaffolds	1478:1490	The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP.					
32279810	9	34	contain	had	1492:1494	arg2	porosity					1503:1510	a high porosity	1496:1510	a high porosity between 89% and 93%	1496:1530	The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP.					
32279810	10	35	theme	NPP	1638:1640	arg1	proportion					1642:1651	higher NPP proportion	1631:1651	higher NPP proportion	1631:1651	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	0	36	theme	acid	38:41	arg1	scaffold					60:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	5	37	theme	transformed	972:982	arg1	Fourier					964:970	Fourier	964:970	Fourier transformed infrared (FTIR)	964:998	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	3	38	theme	C-HA	467:470	arg1	scaffolds					473:481	chitosan-hyaluronic acid (C-HA) scaffolds	441:481	chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP)	441:516	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	8	39	theme	NPP	1393:1395	arg1	content					1397:1403	NPP content	1393:1403	NPP content	1393:1403	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	11	40	theme	Col	1878:1880	arg1	αI					1882:1883	Col αI	1878:1883	Col αI	1878:1883	The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes.					
32279810	0	41	theme	composite	50:58	arg1	scaffold					60:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	3	42	theme	acid	461:464	arg1	scaffolds					473:481	chitosan-hyaluronic acid (C-HA) scaffolds	441:481	chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP)	441:516	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	3	43	theme	current	537:543	arg1	study					545:549	current study	537:549	current study	537:549	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	6	44	theme	cell	1139:1142	arg1	CCK-8					1160:1164	CCK-8	1160:1164	CCK-8	1160:1164	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	6	44	theme	cell	1139:1142	arg1	counting					1144:1151	cell counting kit-8	1139:1157	cell counting kit-8 (CCK-8)	1139:1165	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	2	45	theme	Pearl	238:242	arg1	powder					244:249	Pearl powder	238:249	Pearl powder	238:249	Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties.					
32279810	9	46	theme	NPP	1577:1579	arg1	increase					1565:1572	the increase	1561:1572	the increase of NPP	1561:1579	The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP.					
32279810	4	47	theme	scaffolds	652:660	arg1	microstructure					586:599	The microstructure	582:599	The microstructure	582:599	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	4	47	theme	scaffolds	652:660	arg1	property					640:647	mechanical property	629:647	mechanical property of scaffolds	629:660	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	4	47	theme	scaffolds	652:660	arg1	hydrophilia					612:622	hydrophilia	612:622	hydrophilia	612:622	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	4	47	theme	scaffolds	652:660	arg1	porosity					602:609	porosity	602:609	porosity	602:609	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	3	48	theme	freeze-drying	560:572	arg1	method					574:579	freeze-drying method	560:579	freeze-drying method	560:579	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	6	49	theme	scaffolds	1112:1120	arg1	characteristics					1053:1067	osteogenic characteristics	1042:1067	osteogenic characteristics	1042:1067	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	6	49	theme	scaffolds	1112:1120	arg1	expression					1098:1107	osteogenic related gene expression	1074:1107	osteogenic related gene expression	1074:1107	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	6	49	theme	scaffolds	1112:1120	arg1	biocompatibility					1024:1039	biocompatibility	1024:1039	biocompatibility	1024:1039	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	3	50	contain	containing	483:492	arg1	scaffolds					473:481	chitosan-hyaluronic acid (C-HA) scaffolds	441:481	chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP)	441:516	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	3	50	contain	containing	483:492	arg2	powder					505:510	nano-pearl powder	494:510	nano-pearl powder (NPP)	494:516	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	3	50	contain	containing	483:492	arg2	NPP					513:515	NPP	513:515	NPP	513:515	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	6	51	theme	phosphatase	1177:1187	arg1	ALP					1199:1201	ALP	1199:1201	ALP	1199:1201	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	6	51	theme	phosphatase	1177:1187	arg1	activity					1189:1196	alkaline phosphatase activity	1168:1196	alkaline phosphatase activity (ALP)	1168:1202	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	10	52	theme	MC3T3-E1	1714:1721	arg1	cells					1723:1727	MC3T3-E1 cells	1714:1727	MC3T3-E1 cells	1714:1727	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	1	53	theme	promising	196:204	arg1	approach					206:213	a promising approach	194:213	a promising approach for bone regeneration	194:235	Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration.					
32279810	12	54	theme	powder/chitosan-hyaluronic	1938:1963	arg1	NPP/C-HA					1971:1978	NPP/C-HA	1971:1978	NPP/C-HA	1971:1978	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	12	54	theme	powder/chitosan-hyaluronic	1938:1963	arg1	acid					1965:1968	nano-pearl powder/chitosan-hyaluronic acid	1927:1968	the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds	1923:1989	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	6	55	theme	other	1008:1012	arg1	hand					1014:1017	the other hand	1004:1017	the other hand	1004:1017	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	10	56	theme	10 wt	1749:1753	arg1	%					1754:1754	10 wt%	1749:1754	10 wt%	1749:1754	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	2	57	theme	favorable	344:352	arg1	properties					365:374	favorable mechanical properties	344:374	favorable mechanical properties	344:374	Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties.					
32279810	12	58	theme	tissue	2037:2042	arg1	engineering					2044:2054	bone tissue engineering	2032:2054	bone tissue engineering	2032:2054	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	10	59	theme	Cell	1582:1585	arg1	tests					1595:1599	Cell culture tests	1582:1599	Cell culture tests	1582:1599	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	8	60	dep	hydrophilia	1327:1337	arg1	The					1323:1325	The	1323:1325	The	1323:1325	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	10	61	dep	%	1754:1754	arg1	powder					1778:1783	nano-pearl powder	1767:1783	nano-pearl powder	1767:1783	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	8	62	theme	25	1464:1465	arg1	%					1466:1466	%	1466:1466	%	1466:1466	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	12	63	theme	promising	2006:2014	arg1	biomaterial					2016:2026	a promising biomaterial	2004:2026	a promising biomaterial for bone tissue engineering	2004:2054	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	12	63	theme	promising	2006:2014	arg1	scaffolds					1981:1989	the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds	1923:1989	the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds	1923:1989	Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.					
32279810	1	64	theme	osteoinductive	152:165	arg1	scaffolds					167:175	osteoinductive scaffolds	152:175	osteoinductive scaffolds	152:175	Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration.					
32279810	4	65	theme	universal	755:763	arg1	machine					785:791	universal material experiment machine	755:791	universal material experiment machine respectively	755:804	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	11	66	theme	genes	1905:1909	arg1	expression					1864:1873	the expression	1860:1873	the expression of Col αI, OCN, OPN and Runx2 genes	1860:1909	The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes.					
32279810	5	67	dep	transformed	972:982	arg1	infrared					984:991	infrared	984:991	infrared	984:991	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	0	68	theme	osteogenesis	98:109	arg1	mechanism					111:119	its osteogenesis mechanism	94:119	its osteogenesis mechanism	94:119	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	10	69	with	scaffolds	1734:1742	arg1	%					1754:1754	10 wt%	1749:1754	10 wt%	1749:1754	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	10	69	with	scaffolds	1734:1742	arg1	%					1765:1765	25 wt%	1760:1765	25 wt%	1760:1765	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	4	70	theme	contact	735:741	arg1	angles					743:748	contact angles	735:748	contact angles	735:748	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	4	71	theme	scanning	678:685	arg1	microscopy					696:705	scanning electron microscopy	678:705	scanning electron microscopy (SEM)	678:711	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	4	71	theme	scanning	678:685	arg1	SEM					708:710	SEM	708:710	SEM	708:710	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	6	72	theme	gene	1093:1096	arg1	expression					1098:1107	osteogenic related gene expression	1074:1107	osteogenic related gene expression	1074:1107	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	3	73	theme	bone	395:398	arg1	tissue					400:405	bone tissue	395:405	bone tissue	395:405	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	6	74	dep	biocompatibility	1024:1039	arg1	the					1020:1022	the	1020:1022	the	1020:1022	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	0	75	theme	powder/chitosan-hyaluronic	11:36	arg1	scaffold					60:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	6	76	theme	osteogenic	1074:1083	arg1	expression					1098:1107	osteogenic related gene expression	1074:1107	osteogenic related gene expression	1074:1107	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	3	77	theme	chitosan-hyaluronic	441:459	arg1	scaffolds					473:481	chitosan-hyaluronic acid (C-HA) scaffolds	441:481	chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP)	441:516	In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method.					
32279810	4	78	theme	experiment	774:783	arg1	machine					785:791	universal material experiment machine	755:791	universal material experiment machine respectively	755:804	The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively.					
32279810	10	79	theme	culture	1587:1593	arg1	tests					1595:1599	Cell culture tests	1582:1599	Cell culture tests	1582:1599	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	0	80	theme	porous	43:48	arg1	scaffold					60:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold	0:67	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	1	81	theme	Bone	122:125	arg1	engineering					134:144	Bone tissue engineering	122:144	Bone tissue engineering using osteoinductive scaffolds	122:175	Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration.					
32279810	8	82	theme	scaffolds	1368:1376	arg1	hydrophilia					1327:1337	hydrophilia	1327:1337	hydrophilia	1327:1337	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	8	82	theme	scaffolds	1368:1376	arg1	properties					1354:1363	mechanical properties	1343:1363	mechanical properties	1343:1363	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
32279810	10	83	theme	higher	1631:1636	arg1	proportion					1642:1651	higher NPP proportion	1631:1651	higher NPP proportion	1631:1651	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	5	84	theme	NPP	827:829	arg1	form					819:822	The crystal form	807:822	The crystal form	807:822	The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR).					
32279810	6	85	theme	osteogenic	1042:1051	arg1	characteristics					1053:1067	osteogenic characteristics	1042:1067	osteogenic characteristics	1042:1067	In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting.					
32279810	11	86	theme	αI	1882:1883	arg1	expression					1864:1873	the expression	1860:1873	the expression of Col αI, OCN, OPN and Runx2 genes	1860:1909	The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes.					
32279810	0	87	theme	preliminary	73:83	arg1	study					85:89	preliminary study	73:89	preliminary study of its osteogenesis mechanism	73:119	Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.					
32279810	10	88	theme	25 wt	1760:1764	arg1	%					1765:1765	25 wt%	1760:1765	25 wt%	1760:1765	Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective.					
32279810	8	89	theme	pore	1418:1421	arg1	morphology					1423:1432	the pore morphology	1414:1432	the pore morphology	1414:1432	The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold.					
34894076	9	0	theme	cytokine	1172:1179	arg1	signaling					1181:1189	cytokine signaling 3	1172:1191	cytokine signaling 3	1172:1191	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	10	1	dep	Rodentibacter	1325:1337	arg1	genus					1339:1343	Rodentibacter genus	1325:1343	Rodentibacter genus	1325:1343	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	7	2	theme	lipopolysaccharide-induced	754:779	arg1	ALI					781:783	lipopolysaccharide-induced ALI	754:783	lipopolysaccharide-induced ALI	754:783	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	3	3	from	composition	403:413	arg1	ALI					418:420	ALI	418:420	ALI	418:420	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	3	3	from	composition	403:413	arg1	inflammation					366:377	inflammation	366:377	inflammation	366:377	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	9	4	theme	signaling	1181:1189	arg1	suppressor					1158:1167	suppressor	1158:1167	suppressor of cytokine signaling 3	1158:1191	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	4	theme	signaling	1181:1189	arg1	phosphorylation					1084:1098	the phosphorylation	1080:1098	the phosphorylation of signal transducer and activator of transcription 3	1080:1152	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	7	5	theme	Short-term	707:716	arg1	supplementation					729:743	Short-term vitamin D3 supplementation	707:743	Short-term vitamin D3 supplementation	707:743	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	9	6	theme	long-term	1035:1043	arg1	pretreatment					1045:1056	long-term pretreatment	1035:1056	long-term pretreatment	1035:1056	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	8	7	contain	had	984:986	arg1	treatment					942:950	long-term treatment	932:950	long-term treatment over 3 days, 6 days, or 10 days	932:982	In contrast, long-term treatment over 3 days, 6 days, or 10 days had no such effect.					
34894076	8	7	contain	had	984:986	arg2	effect					996:1001	no such effect	988:1001	no such effect	988:1001	In contrast, long-term treatment over 3 days, 6 days, or 10 days had no such effect.					
34894076	0	8	theme	Rodentibacter	95:107	arg1	abundance					109:117	Lung Rodentibacter abundance	90:117	Lung Rodentibacter abundance	90:117	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	11	9	theme	Correlation	1518:1528	arg1	analysis					1530:1537	Correlation analysis	1518:1537	Correlation analysis	1518:1537	Correlation analysis shows that the load of Rodentibacter positively correlated with the IL-1β, IL-6, and TNF-α gene expression.					
34894076	12	10	theme	adjuvant	1722:1729	arg1	therapy					1731:1737	an adjuvant therapy	1719:1737	an adjuvant therapy for acute lung inflammation	1719:1765	The data support that a single administration of vitamin D3 may work as an adjuvant therapy for acute lung inflammation.					
34894076	0	11	theme	Lung	90:93	arg1	abundance					109:117	Lung Rodentibacter abundance	90:117	Lung Rodentibacter abundance	90:117	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	8	12	theme	long-term	932:940	arg1	treatment					942:950	long-term treatment	932:950	long-term treatment over 3 days, 6 days, or 10 days	932:982	In contrast, long-term treatment over 3 days, 6 days, or 10 days had no such effect.					
34894076	12	13	theme	D3	1704:1705	arg1	administration					1678:1691	a single administration	1669:1691	a single administration of vitamin D3	1669:1705	The data support that a single administration of vitamin D3 may work as an adjuvant therapy for acute lung inflammation.					
34894076	7	14	theme	necrosis	892:899	arg1	factor					901:906	tumor necrosis factor α	886:908	tumor necrosis factor α (TNF-α)	886:916	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	7	14	theme	necrosis	892:899	arg1	TNF-α					911:915	TNF-α	911:915	TNF-α	911:915	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	3	15	theme	different	316:324	arg1	strategies					352:361	different calcitriol administration strategies	316:361	different calcitriol administration strategies	316:361	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	12	16	theme	single	1671:1676	arg1	administration					1678:1691	a single administration	1669:1691	a single administration of vitamin D3	1669:1705	The data support that a single administration of vitamin D3 may work as an adjuvant therapy for acute lung inflammation.					
34894076	10	17	theme	relative	1303:1310	arg1	abundance					1312:1320	an increased relative abundance	1290:1320	an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid	1290:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	3	18	theme	calcitriol	326:335	arg1	strategies					352:361	different calcitriol administration strategies	316:361	different calcitriol administration strategies	316:361	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	8	19	theme	such	991:994	arg1	effect					996:1001	no such effect	988:1001	no such effect	988:1001	In contrast, long-term treatment over 3 days, 6 days, or 10 days had no such effect.					
34894076	7	20	theme	tumor	886:890	arg1	factor					901:906	tumor necrosis factor α	886:908	tumor necrosis factor α (TNF-α)	886:916	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	7	20	theme	tumor	886:890	arg1	TNF-α					911:915	TNF-α	911:915	TNF-α	911:915	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	1	21	theme	severe	164:169	arg1	inflammation					171:182	severe inflammation	164:182	severe inflammation	164:182	Acute lung injury (ALI) is characterized by severe inflammation.					
34894076	4	22	theme	airway	458:463	arg1	pathology					465:473	airway pathology	458:473	airway pathology	458:473	In a mouse model, the alveolus and airway pathology are assessed by immunohistology.					
34894076	10	23	theme	D3	1451:1452	arg1	treatment					1454:1462	short-term vitamin D3 treatment	1432:1462	short-term vitamin D3 treatment	1432:1462	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	2	24	from	inflammation	219:230	arg1	ALI					235:237	ALI	235:237	ALI	235:237	Vitamin D3 is discussed to reduce inflammation in ALI, but the mechanism is not well understood.					
34894076	0	25	theme	Single	0:5	arg1	Treatment					7:15	Single Treatment	0:15	Single Treatment of Vitamin D3	0:29	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	10	26	theme	Rodentibacter	1493:1505	arg1	abundance					1507:1515	the Rodentibacter abundance	1489:1515	the Rodentibacter abundance	1489:1515	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	5	27	theme	protein	572:578	arg1	expressions					580:590	protein expressions	572:590	protein expressions	572:590	mRNA expression is determined by Real-Time Quantitative PCR and protein expressions is detected by Western-blotting.					
34894076	0	28	theme	LPS-Induced	43:53	arg1	Injury					66:71	LPS-Induced Acute Lung Injury	43:71	LPS-Induced Acute Lung Injury	43:71	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	7	29	theme	D3	726:727	arg1	supplementation					729:743	Short-term vitamin D3 supplementation	707:743	Short-term vitamin D3 supplementation	707:743	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	0	30	theme	Vitamin	20:26	arg1	D3					28:29	Vitamin D3	20:29	Vitamin D3	20:29	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	10	31	theme	vitamin	1443:1449	arg1	D3					1451:1452	vitamin D3	1443:1452	short-term vitamin D3 treatment	1432:1462	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	10	32	theme	short-term	1432:1441	arg1	treatment					1454:1462	short-term vitamin D3 treatment	1432:1462	short-term vitamin D3 treatment	1432:1462	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	10	33	theme	Rodentibacter	1325:1337	arg1	abundance					1312:1320	an increased relative abundance	1290:1320	an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid	1290:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	6	34	theme	high-throughput	679:693	arg1	sequencing					695:704	DNA high-throughput sequencing	675:704	DNA high-throughput sequencing	675:704	The composition of microbiota is performed by 16s DNA high-throughput sequencing.					
34894076	9	35	theme	transcription	1138:1150	arg1	activator					1125:1133	activator	1125:1133	activator	1125:1133	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	35	theme	transcription	1138:1150	arg1	transducer					1110:1119	signal transducer	1103:1119	signal transducer	1103:1119	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	7	36	theme	vitamin	718:724	arg1	supplementation					729:743	Short-term vitamin D3 supplementation	707:743	Short-term vitamin D3 supplementation	707:743	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	6	37	theme	DNA	675:677	arg1	sequencing					695:704	DNA high-throughput sequencing	675:704	DNA high-throughput sequencing	675:704	The composition of microbiota is performed by 16s DNA high-throughput sequencing.					
34894076	10	38	dep	mice	1363:1366	arg1	fluid					1391:1395	bronchoalveolar lavage fluid	1368:1395	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	12	39	theme	vitamin	1696:1702	arg1	D3					1704:1705	vitamin D3	1696:1705	vitamin D3	1696:1705	The data support that a single administration of vitamin D3 may work as an adjuvant therapy for acute lung inflammation.					
34894076	4	40	theme	mouse	428:432	arg1	model					434:438	a mouse model	426:438	a mouse model	426:438	In a mouse model, the alveolus and airway pathology are assessed by immunohistology.					
34894076	12	41	theme	lung	1749:1752	arg1	inflammation					1754:1765	acute lung inflammation	1743:1765	acute lung inflammation	1743:1765	The data support that a single administration of vitamin D3 may work as an adjuvant therapy for acute lung inflammation.					
34894076	11	42	theme	IL-1β	1607:1611	arg1	IL-6					1614:1617	the IL-1β, IL-6, and TNF-α gene expression	1603:1644	IL-6	1614:1617	Correlation analysis shows that the load of Rodentibacter positively correlated with the IL-1β, IL-6, and TNF-α gene expression.					
34894076	0	43	theme	D3	28:29	arg1	Treatment					7:15	Single Treatment	0:15	Single Treatment of Vitamin D3	0:29	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	9	44	theme	nuclear	1246:1252	arg1	binding					1268:1274	nuclear factor-κ-gene binding	1246:1274	nuclear factor-κ-gene binding	1246:1274	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	45	theme	activator	1125:1133	arg1	suppressor					1158:1167	suppressor	1158:1167	suppressor of cytokine signaling 3	1158:1191	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	45	theme	activator	1125:1133	arg1	phosphorylation					1084:1098	the phosphorylation	1080:1098	the phosphorylation of signal transducer and activator of transcription 3	1080:1152	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	3	46	theme	lung	387:390	arg1	composition					403:413	the lung microbiota composition	383:413	the lung microbiota composition in ALI	383:420	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	3	47	theme	administration	337:350	arg1	strategies					352:361	different calcitriol administration strategies	316:361	different calcitriol administration strategies	316:361	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	0	48	theme	Lung	61:64	arg1	Injury					66:71	LPS-Induced Acute Lung Injury	43:71	LPS-Induced Acute Lung Injury	43:71	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	10	49	from	abundance	1312:1320	arg1	mice					1363:1366	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	10	50	theme	bronchoalveolar	1368:1382	arg1	fluid					1391:1395	bronchoalveolar lavage fluid	1368:1395	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	1	51	theme	Acute	120:124	arg1	injury					131:136	Acute lung injury	120:136	Acute lung injury (ALI)	120:142	Acute lung injury (ALI) is characterized by severe inflammation.					
34894076	1	51	theme	Acute	120:124	arg1	ALI					139:141	ALI	139:141	ALI	139:141	Acute lung injury (ALI) is characterized by severe inflammation.					
34894076	3	52	theme	microbiota	392:401	arg1	composition					403:413	the lung microbiota composition	383:413	the lung microbiota composition in ALI	383:420	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	9	53	theme	transducer	1110:1119	arg1	suppressor					1158:1167	suppressor	1158:1167	suppressor of cytokine signaling 3	1158:1191	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	53	theme	transducer	1110:1119	arg1	phosphorylation					1084:1098	the phosphorylation	1080:1098	the phosphorylation of signal transducer and activator of transcription 3	1080:1152	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	54	theme	binding	1268:1274	arg1	inhibitor					1233:1241	inhibitor	1233:1241	inhibitor of nuclear factor-κ-gene binding	1233:1274	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	9	55	theme	factor-κ-gene	1254:1266	arg1	binding					1268:1274	nuclear factor-κ-gene binding	1246:1274	nuclear factor-κ-gene binding	1246:1274	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	5	56	theme	Real-Time	541:549	arg1	PCR					564:566	Real-Time Quantitative PCR	541:566	Real-Time Quantitative PCR	541:566	mRNA expression is determined by Real-Time Quantitative PCR and protein expressions is detected by Western-blotting.					
34894076	1	57	theme	lung	126:129	arg1	injury					131:136	Acute lung injury	120:136	Acute lung injury (ALI)	120:142	Acute lung injury (ALI) is characterized by severe inflammation.					
34894076	1	57	theme	lung	126:129	arg1	ALI					139:141	ALI	139:141	ALI	139:141	Acute lung injury (ALI) is characterized by severe inflammation.					
34894076	9	58	theme	Short-term	1004:1013	arg1	D3					1023:1024	Short-term vitamin D3	1004:1024	Short-term vitamin D3	1004:1024	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	12	59	theme	acute	1743:1747	arg1	inflammation					1754:1765	acute lung inflammation	1743:1765	acute lung inflammation	1743:1765	The data support that a single administration of vitamin D3 may work as an adjuvant therapy for acute lung inflammation.					
34894076	5	60	theme	Quantitative	551:562	arg1	PCR					564:566	Real-Time Quantitative PCR	541:566	Real-Time Quantitative PCR	541:566	mRNA expression is determined by Real-Time Quantitative PCR and protein expressions is detected by Western-blotting.					
34894076	11	61	theme	TNF-α	1624:1628	arg1	expression					1635:1644	the IL-1β, IL-6, and TNF-α gene expression	1603:1644	expression	1635:1644	Correlation analysis shows that the load of Rodentibacter positively correlated with the IL-1β, IL-6, and TNF-α gene expression.					
34894076	9	62	theme	vitamin	1015:1021	arg1	D3					1023:1024	Short-term vitamin D3	1004:1024	Short-term vitamin D3	1004:1024	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	7	63	theme	pro-inflammatory	799:814	arg1	factor					901:906	tumor necrosis factor α	886:908	tumor necrosis factor α (TNF-α)	886:916	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	7	63	theme	pro-inflammatory	799:814	arg1	interleukin-6					860:872	interleukin-6	860:872	interleukin-6 (IL-6)	860:879	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	7	63	theme	pro-inflammatory	799:814	arg1	interleukin-1β					836:849	interleukin-1β	836:849	interleukin-1β (IL-1β)	836:857	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	7	63	theme	pro-inflammatory	799:814	arg1	cytokines					816:824	pro-inflammatory cytokines	799:824	pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α)	799:916	Short-term vitamin D3 supplementation prevents lipopolysaccharide-induced ALI by preventing pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α).					
34894076	10	64	theme	LPS-challenged	1348:1361	arg1	mice					1363:1366	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	3	65	from	effect	306:311	arg1	ALI					418:420	ALI	418:420	ALI	418:420	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	3	65	from	effect	306:311	arg1	inflammation					366:377	inflammation	366:377	inflammation	366:377	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	10	66	theme	lavage	1384:1389	arg1	fluid					1391:1395	bronchoalveolar lavage fluid	1368:1395	LPS-challenged mice bronchoalveolar lavage fluid	1348:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	9	67	theme	signal	1103:1108	arg1	transducer					1110:1119	signal transducer	1103:1119	signal transducer	1103:1119	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	10	68	theme	increased	1293:1301	arg1	abundance					1312:1320	an increased relative abundance	1290:1320	an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid	1290:1395	Furthermore, an increased relative abundance of Rodentibacter genus in LPS-challenged mice bronchoalveolar lavage fluid is observed, which is sensitive to short-term vitamin D3 treatment, effectively alleviating the Rodentibacter abundance.					
34894076	2	69	theme	Vitamin	185:191	arg1	D3					193:194	Vitamin D3	185:194	Vitamin D3	185:194	Vitamin D3 is discussed to reduce inflammation in ALI, but the mechanism is not well understood.					
34894076	9	70	theme	inhibitor	1233:1241	arg1	phosphorylation					1214:1228	the phosphorylation	1210:1228	the phosphorylation of inhibitor of nuclear factor-κ-gene binding	1210:1274	Short-term vitamin D3, but not long-term pretreatment significantly reduces the phosphorylation of signal transducer and activator of transcription 3 and suppressor of cytokine signaling 3, but upregulates the phosphorylation of inhibitor of nuclear factor-κ-gene binding.					
34894076	5	71	theme	mRNA	508:511	arg1	expression					513:522	mRNA expression	508:522	mRNA expression	508:522	mRNA expression is determined by Real-Time Quantitative PCR and protein expressions is detected by Western-blotting.					
34894076	6	72	theme	microbiota	644:653	arg1	composition					629:639	The composition	625:639	The composition of microbiota	625:653	The composition of microbiota is performed by 16s DNA high-throughput sequencing.					
34894076	11	73	theme	gene	1630:1633	arg1	expression					1635:1644	the IL-1β, IL-6, and TNF-α gene expression	1603:1644	expression	1635:1644	Correlation analysis shows that the load of Rodentibacter positively correlated with the IL-1β, IL-6, and TNF-α gene expression.					
34894076	0	74	theme	Acute	55:59	arg1	Injury					66:71	LPS-Induced Acute Lung Injury	43:71	LPS-Induced Acute Lung Injury	43:71	Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.					
34894076	11	75	theme	Rodentibacter	1562:1574	arg1	load					1554:1557	the load	1550:1557	the load of Rodentibacter	1550:1574	Correlation analysis shows that the load of Rodentibacter positively correlated with the IL-1β, IL-6, and TNF-α gene expression.					
34894076	3	76	theme	strategies	352:361	arg1	effect					306:311	the effect	302:311	the effect of different calcitriol administration strategies on inflammation	302:377	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
34894076	3	76	theme	strategies	352:361	arg1	composition					403:413	the lung microbiota composition	383:413	the lung microbiota composition in ALI	383:420	This study assesses the effect of different calcitriol administration strategies on inflammation and the lung microbiota composition in ALI.					
32378017	6	0	theme	serine	923:928	arg1	domain					939:944	the serine protease domain	919:944	the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258	919:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	7	1	theme	modification	1139:1150	arg1	site					1131:1134	a site	1129:1134	a site of modification	1129:1150	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	7	1	theme	modification	1139:1150	arg1	modification					1139:1150	modification	1139:1150	modification	1139:1150	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	3	2	from	serine	542:547	arg1	compositions					526:537	previously undescribed O-linked monosaccharide compositions	479:537	previously undescribed O-linked monosaccharide compositions at serine 141	479:551	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	3	2	from	serine	542:547	arg1	glycosylation					573:585	glycosylation	573:585	glycosylation	573:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	3	2	from	serine	542:547	arg1	site					565:568	a novel site	557:568	a novel site of glycosylation	557:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	2	3	theme	Factor	352:357	arg1	concentrates					362:373	plasma-derived or recombinant Factor IX concentrates	322:373	concentrates	362:373	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	4	4	theme	monosaccharide	618:631	arg1	compositions					633:644	two monosaccharide compositions	614:644	two monosaccharide compositions	614:644	At serine 141 we observed two monosaccharide compositions, with HexNAc1Hex1NeuAc2 dominant and a low level of HexNAc1Hex1NeuAc1.					
32378017	7	5	theme	site	1131:1134	arg1	instance					1117:1124	the first reported instance	1098:1124	the first reported instance of a site of modification in the serine protease domain	1098:1180	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	7	5	theme	site	1131:1134	arg1	This					1090:1093	This	1090:1093	This	1090:1093	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	3	6	theme	glycosylation	573:585	arg1	compositions					526:537	previously undescribed O-linked monosaccharide compositions	479:537	previously undescribed O-linked monosaccharide compositions at serine 141	479:551	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	3	6	theme	glycosylation	573:585	arg1	glycosylation					573:585	glycosylation	573:585	glycosylation	573:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	3	6	theme	glycosylation	573:585	arg1	site					565:568	a novel site	557:568	a novel site of glycosylation	557:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	6	7	theme	N-linked	902:909	arg1	site					911:914	an N-linked site	899:914	an N-linked site	899:914	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	7	theme	N-linked	902:909	arg1	site					891:894	The novel site	881:894	The novel site	881:894	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	8	8	theme	functional	1257:1266	arg1	studies					1268:1274	future functional studies	1250:1274	future functional studies	1250:1274	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	2	9	theme	bleeding	267:274	arg1	disorder					276:283	the bleeding disorder	263:283	the bleeding disorder	263:283	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	7	10	theme	reported	1108:1115	arg1	instance					1117:1124	the first reported instance	1098:1124	the first reported instance of a site of modification in the serine protease domain	1098:1180	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	7	10	theme	reported	1108:1115	arg1	This					1090:1093	This	1090:1093	This	1090:1093	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	8	11	theme	events	1222:1227	arg1	description					1187:1197	The description	1183:1197	The description of these glycosylation events	1183:1227	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	6	12	theme	non-canonical	970:982	arg1	motif					994:998	a non-canonical consensus motif	968:998	a non-canonical consensus motif at asparagine 258	968:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	1	13	theme	coagulation	195:205	arg1	cascade					207:213	the blood coagulation cascade	185:213	the blood coagulation cascade	185:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	4	14	theme	low	685:687	arg1	level					689:693	a low level	683:693	a low level of HexNAc1Hex1NeuAc1	683:714	At serine 141 we observed two monosaccharide compositions, with HexNAc1Hex1NeuAc2 dominant and a low level of HexNAc1Hex1NeuAc1.					
32378017	5	15	theme	first	759:763	arg1	site					774:777	the first cleavage site	755:777	the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX	755:878	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	6	16	theme	low	951:953	arg1	occupancy					955:963	low occupancy	951:963	low occupancy in a non-canonical consensus motif at asparagine 258	951:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	3	17	link	O-linked	502:509	arg1	compositions					526:537	previously undescribed O-linked monosaccharide compositions	479:537	previously undescribed O-linked monosaccharide compositions at serine 141	479:551	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	8	18	theme	native	1326:1331	arg1	IX					1340:1341	native Factor IX	1326:1341	native Factor IX for the production of effective therapeutic biosimilars and biobetters	1326:1412	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	5	19	theme	O-linked	722:729	arg1	site					731:734	This O-linked site	717:734	This O-linked site	717:734	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	2	20	theme	Functional	216:225	arg1	deficiencies					227:238	Functional deficiencies	216:238	Functional deficiencies in Factor IX	216:251	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	8	21	theme	IX	1340:1341	arg1	characterisation					1306:1321	structural characterisation	1295:1321	structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters	1295:1412	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	3	22	gly	glycosylation	573:585	arg2	glycosylation					573:585	glycosylation	573:585	glycosylation	573:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	3	22	gly	glycosylation	573:585	arg2	site					565:568	a novel site	557:568	a novel site of glycosylation	557:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	0	23	theme	factor	68:73	arg1	IX					75:76	human serum-derived factor IX	48:76	human serum-derived factor IX	48:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	1	24	theme	important	156:164	arg1	IX					92:93	Human Factor IX	79:93	Human Factor IX	79:93	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	24	theme	important	156:164	arg1	protein					137:143	a highly post-translationally modified protein	98:143	a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade	98:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	24	theme	important	156:164	arg1	factor					175:180	an important clotting factor	153:180	an important clotting factor in the blood coagulation cascade	153:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	0	25	from	Identification	0:13	arg1	IX					75:76	human serum-derived factor IX	48:76	human serum-derived factor IX	48:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	8	26	theme	therapeutic	1375:1385	arg1	biosimilars					1387:1397	effective therapeutic biosimilars	1365:1397	effective therapeutic biosimilars	1365:1397	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	2	27	theme	haemophilia	285:295	arg1	B					297:297	haemophilia B	285:297	haemophilia B	285:297	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	2	28	from	deficiencies	227:238	arg1	IX					250:251	Factor IX	243:251	Factor IX	243:251	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	3	29	theme	Factor	458:463	arg1	IX					465:466	human serum-derived Factor IX	438:466	human serum-derived Factor IX	438:466	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	5	30	theme	important	810:818	arg1	peptide					798:804	the activation peptide	783:804	the activation peptide	783:804	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	5	30	theme	important	810:818	arg1	region					820:825	an important region	807:825	an important region of the protein that is removed to activate Factor IX	807:878	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	1	31	theme	Human	79:83	arg1	IX					92:93	Human Factor IX	79:93	Human Factor IX	79:93	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	31	theme	Human	79:83	arg1	protein					137:143	a highly post-translationally modified protein	98:143	a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade	98:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	31	theme	Human	79:83	arg1	factor					175:180	an important clotting factor	153:180	an important clotting factor in the blood coagulation cascade	153:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	0	32	from	events	38:43	arg1	IX					75:76	human serum-derived factor IX	48:76	human serum-derived factor IX	48:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	3	33	theme	human	438:442	arg1	IX					465:466	human serum-derived Factor IX	438:466	human serum-derived Factor IX	438:466	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	0	34	theme	novel	18:22	arg1	events					38:43	novel glycosylation events	18:43	novel glycosylation events on human serum-derived factor IX	18:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	2	35	dep	disorder	276:283	arg1	B					297:297	haemophilia B	285:297	haemophilia B	285:297	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	7	36	theme	protease	1166:1173	arg1	domain					1175:1180	the serine protease domain	1155:1180	the serine protease domain	1155:1180	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	0	37	theme	events	38:43	arg1	Identification					0:13	Identification	0:13	Identification of novel glycosylation events on human serum-derived factor IX.	0:77	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	6	38	theme	HexNAc4Hex5NeuAc2	1035:1051	arg1	composition					1068:1078	a HexNAc4Hex5NeuAc2 monosaccharide composition	1033:1078	a HexNAc4Hex5NeuAc2 monosaccharide composition attached	1033:1087	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	2	39	theme	recombinant	340:350	arg1	concentrates					362:373	plasma-derived or recombinant Factor IX concentrates	322:373	concentrates	362:373	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	0	40	theme	human	48:52	arg1	IX					75:76	human serum-derived factor IX	48:76	human serum-derived factor IX	48:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	6	41	from	occupancy	955:963	arg1	asparagine					1003:1012	asparagine 258	1003:1016	asparagine 258	1003:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	41	from	occupancy	955:963	arg1	motif					994:998	a non-canonical consensus motif	968:998	a non-canonical consensus motif at asparagine 258	968:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	3	42	theme	O-linked	502:509	arg1	compositions					526:537	previously undescribed O-linked monosaccharide compositions	479:537	previously undescribed O-linked monosaccharide compositions at serine 141	479:551	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	6	43	theme	protease	930:937	arg1	domain					939:944	the serine protease domain	919:944	the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258	919:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	2	44	theme	IX	359:360	arg1	concentrates					362:373	plasma-derived or recombinant Factor IX concentrates	322:373	concentrates	362:373	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	6	45	from	site	911:914	arg1	domain					939:944	the serine protease domain	919:944	the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258	919:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	3	46	theme	novel	559:563	arg1	glycosylation					573:585	glycosylation	573:585	glycosylation	573:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	3	46	theme	novel	559:563	arg1	site					565:568	a novel site	557:568	a novel site of glycosylation	557:585	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	1	47	theme	clotting	166:173	arg1	IX					92:93	Human Factor IX	79:93	Human Factor IX	79:93	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	47	theme	clotting	166:173	arg1	protein					137:143	a highly post-translationally modified protein	98:143	a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade	98:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	47	theme	clotting	166:173	arg1	factor					175:180	an important clotting factor	153:180	an important clotting factor in the blood coagulation cascade	153:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	3	48	link	serum-derived	444:456	arg1	IX					465:466	human serum-derived Factor IX	438:466	human serum-derived Factor IX	438:466	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	0	49	link	serum-derived	54:66	arg1	IX					75:76	human serum-derived factor IX	48:76	human serum-derived factor IX	48:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	8	50	theme	future	1250:1255	arg1	studies					1268:1274	future functional studies	1250:1274	future functional studies	1250:1274	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	6	51	theme	consensus	984:992	arg1	motif					994:998	a non-canonical consensus motif	968:998	a non-canonical consensus motif at asparagine 258	968:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	52	with	domain	939:944	arg1	occupancy					955:963	low occupancy	951:963	low occupancy in a non-canonical consensus motif at asparagine 258	951:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	4	53	theme	HexNAc1Hex1NeuAc1	698:714	arg1	level					689:693	a low level	683:693	a low level of HexNAc1Hex1NeuAc1	683:714	At serine 141 we observed two monosaccharide compositions, with HexNAc1Hex1NeuAc2 dominant and a low level of HexNAc1Hex1NeuAc1.					
32378017	4	53	theme	HexNAc1Hex1NeuAc1	698:714	arg1	HexNAc1Hex1NeuAc2					652:668	HexNAc1Hex1NeuAc2 dominant	652:677	HexNAc1Hex1NeuAc2 dominant	652:677	At serine 141 we observed two monosaccharide compositions, with HexNAc1Hex1NeuAc2 dominant and a low level of HexNAc1Hex1NeuAc1.					
32378017	1	54	theme	blood	189:193	arg1	cascade					207:213	the blood coagulation cascade	185:213	the blood coagulation cascade	185:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	8	55	theme	glycosylation	1208:1220	arg1	events					1222:1227	these glycosylation events	1202:1227	these glycosylation events	1202:1227	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	4	56	theme	dominant	670:677	arg1	HexNAc1Hex1NeuAc2					652:668	HexNAc1Hex1NeuAc2 dominant	652:677	HexNAc1Hex1NeuAc2 dominant	652:677	At serine 141 we observed two monosaccharide compositions, with HexNAc1Hex1NeuAc2 dominant and a low level of HexNAc1Hex1NeuAc1.					
32378017	2	57	theme	Factor	243:248	arg1	IX					250:251	Factor IX	243:251	Factor IX	243:251	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	5	58	theme	Factor	870:875	arg1	IX					877:878	Factor IX	870:878	Factor IX	870:878	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	0	59	from	IX	75:76	arg1	Identification					0:13	Identification	0:13	Identification of novel glycosylation events on human serum-derived factor IX.	0:77	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	8	60	theme	structural	1295:1304	arg1	characterisation					1306:1321	structural characterisation	1295:1321	structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters	1295:1412	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	1	61	theme	modified	128:135	arg1	IX					92:93	Human Factor IX	79:93	Human Factor IX	79:93	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	61	theme	modified	128:135	arg1	protein					137:143	a highly post-translationally modified protein	98:143	a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade	98:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	61	theme	modified	128:135	arg1	factor					175:180	an important clotting factor	153:180	an important clotting factor in the blood coagulation cascade	153:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	8	62	theme	Factor	1333:1338	arg1	IX					1340:1341	native Factor IX	1326:1341	native Factor IX for the production of effective therapeutic biosimilars and biobetters	1326:1412	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	2	63	theme	plasma-derived	322:335	arg1	concentrates					362:373	plasma-derived or recombinant Factor IX concentrates	322:373	concentrates	362:373	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
32378017	5	64	theme	activation	787:796	arg1	peptide					798:804	the activation peptide	783:804	the activation peptide	783:804	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	5	64	theme	activation	787:796	arg1	region					820:825	an important region	807:825	an important region of the protein that is removed to activate Factor IX	807:878	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	1	65	from	factor	175:180	arg1	cascade					207:213	the blood coagulation cascade	185:213	the blood coagulation cascade	185:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	5	66	theme	cleavage	765:772	arg1	site					774:777	the first cleavage site	755:777	the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX	755:878	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	3	67	theme	monosaccharide	511:524	arg1	compositions					526:537	previously undescribed O-linked monosaccharide compositions	479:537	previously undescribed O-linked monosaccharide compositions at serine 141	479:551	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	8	68	theme	biosimilars	1387:1397	arg1	production					1351:1360	the production	1347:1360	the production of effective therapeutic biosimilars and biobetters	1347:1412	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	3	69	theme	IX	465:466	arg1	modifications					421:433	the post-translational modifications	398:433	the post-translational modifications of human serum-derived Factor IX	398:466	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	5	70	theme	protein	834:840	arg1	peptide					798:804	the activation peptide	783:804	the activation peptide	783:804	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	5	70	theme	protein	834:840	arg1	region					820:825	an important region	807:825	an important region of the protein that is removed to activate Factor IX	807:878	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	1	71	theme	Factor	85:90	arg1	IX					92:93	Human Factor IX	79:93	Human Factor IX	79:93	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	71	theme	Factor	85:90	arg1	protein					137:143	a highly post-translationally modified protein	98:143	a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade	98:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	1	71	theme	Factor	85:90	arg1	factor					175:180	an important clotting factor	153:180	an important clotting factor in the blood coagulation cascade	153:213	Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade.					
32378017	6	72	from	asparagine	1003:1012	arg1	motif					994:998	a non-canonical consensus motif	968:998	a non-canonical consensus motif at asparagine 258	968:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	72	from	asparagine	1003:1012	arg1	occupancy					955:963	low occupancy	951:963	low occupancy in a non-canonical consensus motif at asparagine 258	951:1016	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	8	73	theme	biobetters	1403:1412	arg1	production					1351:1360	the production	1347:1360	the production of effective therapeutic biosimilars and biobetters	1347:1412	The description of these glycosylation events provides a basis for future functional studies and contributes to structural characterisation of native Factor IX for the production of effective therapeutic biosimilars and biobetters.					
32378017	3	74	theme	serum-derived	444:456	arg1	IX					465:466	human serum-derived Factor IX	438:466	human serum-derived Factor IX	438:466	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	0	75	theme	glycosylation	24:36	arg1	events					38:43	novel glycosylation events	18:43	novel glycosylation events on human serum-derived factor IX	18:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	7	76	from	instance	1117:1124	arg1	domain					1175:1180	the serine protease domain	1155:1180	the serine protease domain	1155:1180	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	3	77	theme	undescribed	490:500	arg1	compositions					526:537	previously undescribed O-linked monosaccharide compositions	479:537	previously undescribed O-linked monosaccharide compositions at serine 141	479:551	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	7	78	theme	serine	1159:1164	arg1	domain					1175:1180	the serine protease domain	1155:1180	the serine protease domain	1155:1180	This is the first reported instance of a site of modification in the serine protease domain.					
32378017	6	79	theme	novel	885:889	arg1	site					911:914	an N-linked site	899:914	an N-linked site	899:914	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	79	theme	novel	885:889	arg1	site					891:894	The novel site	881:894	The novel site	881:894	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	80	theme	monosaccharide	1053:1066	arg1	composition					1068:1078	a HexNAc4Hex5NeuAc2 monosaccharide composition	1033:1078	a HexNAc4Hex5NeuAc2 monosaccharide composition attached	1033:1087	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	81	link	N-linked	902:909	arg1	site					911:914	an N-linked site	899:914	an N-linked site	899:914	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	6	81	link	N-linked	902:909	arg1	site					891:894	The novel site	881:894	The novel site	881:894	The novel site is an N-linked site in the serine protease domain with low occupancy in a non-canonical consensus motif at asparagine 258, observed with a HexNAc4Hex5NeuAc2 monosaccharide composition attached.					
32378017	0	82	theme	serum-derived	54:66	arg1	IX					75:76	human serum-derived factor IX	48:76	human serum-derived factor IX	48:76	Identification of novel glycosylation events on human serum-derived factor IX.					
32378017	3	83	theme	post-translational	402:419	arg1	modifications					421:433	the post-translational modifications	398:433	the post-translational modifications of human serum-derived Factor IX	398:466	Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and a novel site of glycosylation.					
32378017	5	84	link	O-linked	722:729	arg1	site					731:734	This O-linked site	717:734	This O-linked site	717:734	This O-linked site lies N-terminal to the first cleavage site for the activation peptide, an important region of the protein that is removed to activate Factor IX.					
32378017	2	85	link	plasma-derived	322:335	arg1	concentrates					362:373	plasma-derived or recombinant Factor IX concentrates	322:373	concentrates	362:373	Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates.					
33625790	7	0	dep	in	1233:1234	arg1	vivo					1236:1239	vivo	1236:1239	vivo	1236:1239	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	1	theme	activated	1287:1295	arg1	fibers					1302:1307	activated gene fibers	1287:1307	activated gene fibers	1287:1307	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	6	2	theme	angiogenic-related	1164:1181	arg1	expression					1189:1198	osteogenic-related and angiogenic-related genes expression	1141:1198	osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells	1141:1216	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	4	3	theme	composite	706:714	arg1	scaffold					716:723	the resulting composite scaffold	692:723	the resulting composite scaffold	692:723	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	7	4	theme	composite	1263:1271	arg1	scaffold					1273:1280	the composite scaffold	1259:1280	the composite scaffold with activated gene fibers	1259:1307	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	5	theme	composite	1458:1466	arg1	scaffolds					1477:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	5	theme	composite	1458:1466	arg1	candidates					1501:1510	excellent candidates	1491:1510	excellent candidates for bone repair materials	1491:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	6	theme	hydrogel	1468:1475	arg1	scaffolds					1477:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	6	theme	hydrogel	1468:1475	arg1	candidates					1501:1510	excellent candidates	1491:1510	excellent candidates for bone repair materials	1491:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	5	7	theme	protein-2	917:925	arg1	release					887:893	sustained release	877:893	sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization	877:1017	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	1	8	theme	bone	267:270	arg1	engineering					279:289	bone tissue engineering	267:289	bone tissue engineering	267:289	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	0	9	theme	In	90:91	arg1	Regeneration					103:114	In Situ Bone Regeneration	90:114	In Situ Bone Regeneration	90:114	Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.					
33625790	1	10	theme	tissue	272:277	arg1	engineering					279:289	bone tissue engineering	267:289	bone tissue engineering	267:289	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	3	11	theme	methacryloyl	498:509	arg1	system					548:553	gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system	490:553	gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure	490:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	5	12	theme	composite	849:857	arg1	scaffold					859:866	the fabricated composite scaffold	834:866	the fabricated composite scaffold	834:866	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	3	13	theme	chitosan	533:540	arg1	system					548:553	gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system	490:553	gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure	490:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	1	14	theme	strong	176:181	arg1	resistance					183:192	strong resistance	176:192	strong resistance to cell adhesion	176:209	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	0	15	theme	Bone	98:101	arg1	Regeneration					103:114	In Situ Bone Regeneration	90:114	In Situ Bone Regeneration	90:114	Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.					
33625790	7	16	theme	bone	1401:1404	arg1	regeneration					1406:1417	bone regeneration	1401:1417	bone regeneration	1401:1417	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	0	17	dep	In	90:91	arg1	Situ					93:96	Situ	93:96	Situ	93:96	Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.					
33625790	6	18	theme	osteogenic-related	1141:1158	arg1	expression					1189:1198	osteogenic-related and angiogenic-related genes expression	1141:1198	osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells	1141:1216	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	2	19	theme	osteogenic	398:407	arg1	engineering					339:349	a bone tissue engineering	325:349	a bone tissue engineering material with both good mechanical properties	325:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	2	19	theme	osteogenic	398:407	arg1	activity					424:431	osteogenic and angiogenic activity	398:431	osteogenic and angiogenic activity	398:431	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	3	20	theme	thiolated	523:531	arg1	TCS					543:545	TCS	543:545	TCS	543:545	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	20	theme	thiolated	523:531	arg1	chitosan					533:540	thiolated chitosan	523:540	thiolated chitosan (TCS)	523:546	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	7	21	theme	gene	1297:1300	arg1	fibers					1302:1307	activated gene fibers	1287:1307	activated gene fibers	1287:1307	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	22	theme	bone	1516:1519	arg1	materials					1528:1536	bone repair materials	1516:1536	bone repair materials	1516:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	23	theme	regeneration	1406:1417	arg1	meaning					1420:1426	meaning	1420:1426	meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials	1420:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	23	theme	regeneration	1406:1417	arg1	capacity					1389:1396	favorable capacity	1379:1396	favorable capacity of bone regeneration	1379:1417	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	0	24	theme	Biomimetic	0:9	arg1	Hydrogels					11:19	Biomimetic Hydrogels	0:19	Biomimetic Hydrogels Loaded with Nanofibers	0:42	Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.					
33625790	5	25	theme	sustained	877:885	arg1	release					887:893	sustained release	877:893	sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization	877:1017	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	7	26	theme	repair	1521:1526	arg1	materials					1528:1536	bone repair materials	1516:1536	bone repair materials	1516:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	1	27	theme	cell	197:200	arg1	adhesion					202:209	cell adhesion	197:209	cell adhesion	197:209	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	7	28	theme	in	1233:1234	arg1	results					1241:1247	the in vivo results	1229:1247	the in vivo results	1229:1247	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	6	29	theme	cell	1024:1027	arg1	experiment					1029:1038	The cell experiment	1020:1038	The cell experiment	1020:1038	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	3	30	theme	GelMA/TCS/pNF	577:589	arg1	hydrogel					601:608	a novel GelMA/TCS/pNF composite hydrogel	569:608	a novel GelMA/TCS/pNF composite hydrogel with dual network structure	569:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	31	with	hydrogel	601:608	arg1	structure					628:636	dual network structure	615:636	dual network structure	615:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	5	32	theme	fabricated	838:847	arg1	scaffold					859:866	the fabricated composite scaffold	834:866	the fabricated composite scaffold	834:866	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	4	33	dep	shows	725:729	arg1	enhanced					739:746	enhanced	739:746	shows greatly enhanced mechanical strength (0.53 MPa)	725:777	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	4	34	theme	resulting	696:704	arg1	scaffold					716:723	the resulting composite scaffold	692:723	the resulting composite scaffold	692:723	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	5	35	theme	osteogenic	943:952	arg1	differentiation					954:968	differentiation	954:968	differentiation	954:968	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	1	36	from	application	252:262	arg1	engineering					279:289	bone tissue engineering	267:289	bone tissue engineering	267:289	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	6	37	theme	MC3T3-E1	1203:1210	arg1	cells					1212:1216	MC3T3-E1 cells	1203:1216	MC3T3-E1 cells	1203:1216	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	3	38	theme	composite	591:599	arg1	hydrogel					601:608	a novel GelMA/TCS/pNF composite hydrogel	569:608	a novel GelMA/TCS/pNF composite hydrogel with dual network structure	569:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	39	theme	DNA	454:456	arg1	plasmid					458:464	DNA plasmid	454:464	DNA plasmid (pNF)	454:470	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	39	theme	DNA	454:456	arg1	pNF					467:469	pNF	467:469	pNF	467:469	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	7	40	theme	favorable	1379:1387	arg1	meaning					1420:1426	meaning	1420:1426	meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials	1420:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	40	theme	favorable	1379:1387	arg1	capacity					1389:1396	favorable capacity	1379:1396	favorable capacity of bone regeneration	1379:1417	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	4	41	theme	compressive	666:676	arg1	measurements					678:689	the compressive measurements	662:689	the compressive measurements	662:689	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	2	42	with	material	351:358	arg1	properties					386:395	both good mechanical properties	365:395	both good mechanical properties	365:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	4	43	theme	compression	817:827	arg1	cycles					807:812	20 cycles	804:812	20 cycles of compression	804:827	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	1	44	theme	weak	216:219	arg1	bioactivity					221:231	weak bioactivity	216:231	weak bioactivity which limits their application in bone tissue engineering considerably	216:302	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	5	45	theme	morphogenetic	903:915	arg1	protein-2					917:925	bone morphogenetic protein-2	898:925	bone morphogenetic protein-2	898:925	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	6	46	theme	cell	1098:1101	arg1	attachment					1103:1112	MC3T3-E1 cell attachment	1089:1112	MC3T3-E1 cell attachment	1089:1112	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	3	47	theme	novel	571:575	arg1	hydrogel					601:608	a novel GelMA/TCS/pNF composite hydrogel	569:608	a novel GelMA/TCS/pNF composite hydrogel with dual network structure	569:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	6	48	theme	genes	1183:1187	arg1	expression					1189:1198	osteogenic-related and angiogenic-related genes expression	1141:1198	osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells	1141:1216	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	2	49	theme	material	351:358	arg1	engineering					339:349	a bone tissue engineering	325:349	a bone tissue engineering material with both good mechanical properties	325:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	2	49	theme	material	351:358	arg1	activity					424:431	osteogenic and angiogenic activity	398:431	osteogenic and angiogenic activity	398:431	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	0	50	theme	Sustained	52:60	arg1	Release					62:68	Sustained Release	52:68	Sustained Release of pDNA	52:76	Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.					
33625790	1	51	theme	insufficient	149:160	arg1	hydrogels					125:133	Polymer hydrogels	117:133	Polymer hydrogels	117:133	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	1	51	theme	insufficient	149:160	arg1	biomechanics					162:173	generally insufficient biomechanics	139:173	generally insufficient biomechanics	139:173	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	1	52	theme	Polymer	117:123	arg1	hydrogels					125:133	Polymer hydrogels	117:133	Polymer hydrogels	117:133	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	1	52	theme	Polymer	117:123	arg1	biomechanics					162:173	generally insufficient biomechanics	139:173	generally insufficient biomechanics	139:173	Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably.					
33625790	7	53	with	scaffold	1273:1280	arg1	fibers					1302:1307	activated gene fibers	1287:1307	activated gene fibers	1287:1307	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	54	theme	resulting	1437:1445	arg1	scaffolds					1477:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	54	theme	resulting	1437:1445	arg1	candidates					1501:1510	excellent candidates	1491:1510	excellent candidates for bone repair materials	1491:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	3	55	contain	carrying	445:452	arg2	plasmid					458:464	DNA plasmid	454:464	DNA plasmid (pNF)	454:470	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	55	contain	carrying	445:452	arg2	pNF					467:469	pNF	467:469	pNF	467:469	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	55	contain	carrying	445:452	arg1	Nanofibers					434:443	Nanofibers	434:443	Nanofibers carrying DNA plasmid (pNF)	434:470	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	56	theme	network	620:626	arg1	structure					628:636	dual network structure	615:636	dual network structure	615:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	7	57	theme	excellent	1491:1499	arg1	scaffolds					1477:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	57	theme	excellent	1491:1499	arg1	candidates					1501:1510	excellent candidates	1491:1510	excellent candidates for bone repair materials	1491:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	2	58	theme	tissue	332:337	arg1	engineering					339:349	a bone tissue engineering	325:349	a bone tissue engineering material with both good mechanical properties	325:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	2	58	theme	tissue	332:337	arg1	activity					424:431	osteogenic and angiogenic activity	398:431	osteogenic and angiogenic activity	398:431	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	5	59	theme	bone	898:901	arg1	protein-2					917:925	bone morphogenetic protein-2	898:925	bone morphogenetic protein-2	898:925	And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization.					
33625790	6	60	theme	MC3T3-E1	1089:1096	arg1	attachment					1103:1112	MC3T3-E1 cell attachment	1089:1112	MC3T3-E1 cell attachment	1089:1112	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	7	61	theme	biomimetic	1447:1456	arg1	scaffolds					1477:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	the resulting biomimetic composite hydrogel scaffolds	1433:1485	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	7	61	theme	biomimetic	1447:1456	arg1	candidates					1501:1510	excellent candidates	1491:1510	excellent candidates for bone repair materials	1491:1536	Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials.					
33625790	2	62	theme	bone	327:330	arg1	engineering					339:349	a bone tissue engineering	325:349	a bone tissue engineering material with both good mechanical properties	325:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	2	62	theme	bone	327:330	arg1	activity					424:431	osteogenic and angiogenic activity	398:431	osteogenic and angiogenic activity	398:431	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	6	63	theme	cells	1212:1216	arg1	proliferation					1115:1127	proliferation	1115:1127	proliferation	1115:1127	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	6	63	theme	cells	1212:1216	arg1	attachment					1103:1112	MC3T3-E1 cell attachment	1089:1112	MC3T3-E1 cell attachment	1089:1112	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	6	63	theme	cells	1212:1216	arg1	expression					1189:1198	osteogenic-related and angiogenic-related genes expression	1141:1198	osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells	1141:1216	The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells.					
33625790	4	64	theme	mechanical	748:757	arg1	0.53 MPa					769:776	0.53 MPa	769:776	0.53 MPa	769:776	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	4	64	theme	mechanical	748:757	arg1	strength					759:766	mechanical strength	748:766	mechanical strength (0.53 MPa)	748:777	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	0	65	theme	pDNA	73:76	arg1	Release					62:68	Sustained Release	52:68	Sustained Release of pDNA	52:76	Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.					
33625790	2	66	theme	angiogenic	413:422	arg1	engineering					339:349	a bone tissue engineering	325:349	a bone tissue engineering material with both good mechanical properties	325:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	2	66	theme	angiogenic	413:422	arg1	activity					424:431	osteogenic and angiogenic activity	398:431	osteogenic and angiogenic activity	398:431	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	2	67	theme	mechanical	375:384	arg1	properties					386:395	both good mechanical properties	365:395	both good mechanical properties	365:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
33625790	3	68	theme	gelatin	490:496	arg1	methacryloyl					498:509	gelatin methacryloyl	490:509	gelatin methacryloyl (GelMA)	490:517	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	3	68	theme	gelatin	490:496	arg1	GelMA					512:516	GelMA	512:516	GelMA	512:516	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	4	69	theme	measurements	678:689	arg1	characterization					642:657	characterization	642:657	characterization of the compressive measurements	642:689	By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression.					
33625790	3	70	theme	dual	615:618	arg1	structure					628:636	dual network structure	615:636	dual network structure	615:636	Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure.					
33625790	2	71	theme	good	370:373	arg1	properties					386:395	both good mechanical properties	365:395	both good mechanical properties	365:395	In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity.					
34735907	6	0	theme	active	504:509	arg1	composition					522:532	the active ingredient composition	500:532	the active ingredient composition of JXR	500:539	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	5	1	theme	chronic	402:408	arg1	disorder					410:417	a chronic disorder	400:417	a chronic disorder of the human gastrointestinal tract	400:453	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	5	1	theme	chronic	402:408	arg1	disease					383:389	Inflammatory bowel disease	364:389	Inflammatory bowel disease (IBD)	364:395	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	6	2	from	composition	522:532	arg1	IBD					492:494	IBD	492:494	IBD	492:494	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	19	3	theme	present	2312:2318	arg1	study					2320:2324	the present study	2308:2324	the present study	2308:2324	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	7	4	theme	anti-inflammatory	695:711	arg1	mechanisms					713:722	the possible anti-inflammatory mechanisms	682:722	the possible anti-inflammatory mechanisms	682:722	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	15	5	theme	antioxidative	1735:1747	arg1	enzyme					1749:1754	antioxidative enzyme	1735:1754	antioxidative enzyme	1735:1754	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	6	dep	mediators	1798:1806	arg1	PGE2					1813:1816	PGE2	1813:1816	PGE2	1813:1816	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	6	dep	mediators	1798:1806	arg1	NO					1809:1810	NO	1809:1810	NO	1809:1810	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	11	7	dep	mediators	1026:1034	arg1	NO					1051:1052	NO	1051:1052	NO	1051:1052	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	7	dep	mediators	1026:1034	arg1	oxide					1044:1048	nitric oxide	1037:1048	nitric oxide (NO)	1037:1053	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	7	dep	mediators	1026:1034	arg1	E2					1070:1071	prostaglandin E2	1056:1071	prostaglandin E2 (PGE2)	1056:1078	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	8	theme	prostaglandin	1056:1068	arg1	oxide					1044:1048	nitric oxide	1037:1048	nitric oxide (NO)	1037:1053	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	8	theme	prostaglandin	1056:1068	arg1	PGE2					1074:1077	PGE2	1074:1077	PGE2	1074:1077	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	8	theme	prostaglandin	1056:1068	arg1	E2					1070:1071	prostaglandin E2	1056:1071	prostaglandin E2 (PGE2)	1056:1078	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	13	9	dep	RESULTS	1312:1318	arg1	constitutes					1331:1341	constitutes	1331:1341	constitutes	1331:1341	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	4	10	theme	diseases	354:361	arg1	therapy					289:295	the therapy	285:295	the therapy of cold, fever, diarrhea, digestive disorders, and other diseases	285:361	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	5	11	theme	tract	449:453	arg1	disorder					410:417	a chronic disorder	400:417	a chronic disorder of the human gastrointestinal tract	400:453	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	5	11	theme	tract	449:453	arg1	disease					383:389	Inflammatory bowel disease	364:389	Inflammatory bowel disease (IBD)	364:395	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	17	12	theme	microbial	1989:1997	arg1	diversity					1999:2007	the microbial diversity	1985:2007	the microbial diversity	1985:2007	Furthermore, JXR could restore the microbial diversity by suppressing Bacteroidaceae, increasing Bifidobacteriales and Melainabacteria in DSS colitis mouse model.					
34735907	14	13	theme	mice	1518:1521	arg1	model					1523:1527	DSS-induced colitis mice model	1498:1527	DSS-induced colitis mice model	1498:1527	The results of DSS-induced colitis mice model indicated that JXR effectively ameliorated inflammation, restore the redox balance in the gut.					
34735907	15	14	theme	mediators	1798:1806	arg1	secretion					1772:1780	the secretion	1768:1780	the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β)	1768:1852	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	16	15	theme	kinases	1919:1925	arg1	pathway					1945:1951	mitogen-activated protein kinases (MAPKs) signaling pathway	1893:1951	mitogen-activated protein kinases (MAPKs) signaling pathway	1893:1951	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	7	16	theme	JXR	666:668	arg1	mechanisms					713:722	the possible anti-inflammatory mechanisms	682:722	the possible anti-inflammatory mechanisms	682:722	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	7	16	theme	JXR	666:668	arg1	property					654:661	the anti-inflammatory property	632:661	the anti-inflammatory property of JXR	632:668	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	7	16	theme	JXR	666:668	arg1	composition					616:626	the phytochemical composition	598:626	the phytochemical composition	598:626	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	11	17	theme	mediators	1026:1034	arg1	content					1002:1008	The content	998:1008	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β))	998:1176	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	17	theme	mediators	1026:1034	arg1	levels					1221:1226	protein expression levels	1202:1226	protein expression levels	1202:1226	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	17	theme	mediators	1026:1034	arg1	mRNA					1193:1196	corresponding mRNA	1179:1196	corresponding mRNA	1179:1196	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	14	18	theme	DSS-induced	1498:1508	arg1	model					1523:1527	DSS-induced colitis mice model	1498:1527	DSS-induced colitis mice model	1498:1527	The results of DSS-induced colitis mice model indicated that JXR effectively ameliorated inflammation, restore the redox balance in the gut.					
34735907	15	19	theme	oxygen	1687:1692	arg1	ROS					1703:1705	ROS	1703:1705	ROS	1703:1705	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	19	theme	oxygen	1687:1692	arg1	species					1694:1700	reactive oxygen species	1678:1700	reactive oxygen species (ROS)	1678:1706	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	8	20	theme	JXR	758:760	arg1	extracts					762:769	JXR extracts	758:769	JXR extracts	758:769	METHODS The bioactive profile of JXR extracts was determined by UPLC-LTQ-Orbitrap-MS.					
34735907	14	21	theme	redox	1598:1602	arg1	balance					1604:1610	the redox balance	1594:1610	the redox balance	1594:1610	The results of DSS-induced colitis mice model indicated that JXR effectively ameliorated inflammation, restore the redox balance in the gut.					
34735907	1	22	theme	colitis	163:169	arg1	mice					171:174	DSS-induced colitis mice	151:174	DSS-induced colitis mice	151:174	Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice.					
34735907	12	23	theme	microbial	1270:1278	arg1	composition					1280:1290	gut microbial composition	1266:1290	gut microbial composition	1266:1290	Oxidation pressure and gut microbial composition were also explored.					
34735907	11	24	theme	expression	1210:1219	arg1	levels					1221:1226	protein expression levels	1202:1226	protein expression levels	1202:1226	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	16	25	theme	protein	1911:1917	arg1	MAPKs					1928:1932	MAPKs	1928:1932	MAPKs	1928:1932	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	16	25	theme	protein	1911:1917	arg1	kinases					1919:1925	mitogen-activated protein kinases	1893:1925	mitogen-activated protein kinases (MAPKs) signaling pathway	1893:1951	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	19	26	theme	functional	2494:2503	arg1	JXR					2485:2487	JXR	2485:2487	JXR	2485:2487	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	19	26	theme	functional	2494:2503	arg1	ingredient					2505:2514	a functional ingredient	2492:2514	a functional ingredient to promote colon health	2492:2538	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	11	27	theme	nitric	1037:1042	arg1	NO					1051:1052	NO	1051:1052	NO	1051:1052	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	27	theme	nitric	1037:1042	arg1	oxide					1044:1048	nitric oxide	1037:1048	nitric oxide (NO)	1037:1053	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	27	theme	nitric	1037:1042	arg1	E2					1070:1071	prostaglandin E2	1056:1071	prostaglandin E2 (PGE2)	1056:1078	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	4	28	theme	traditional	237:247	arg1	medicine					257:264	a traditional Chinese medicine	235:264	a traditional Chinese medicine	235:264	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	4	28	theme	traditional	237:247	arg1	Jiangxiangru					215:226	Jiangxiangru	215:226	Jiangxiangru (JXR)	215:232	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	9	29	theme	induced	817:823	arg1	model					839:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model was applied to explore the anti-inflammatory activity of JXR.					
34735907	1	30	theme	intestinal	126:135	arg1	microbiota					137:146	intestinal microbiota	126:146	intestinal microbiota	126:146	Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice.					
34735907	4	31	theme	cold	300:303	arg1	therapy					289:295	the therapy	285:295	the therapy of cold, fever, diarrhea, digestive disorders, and other diseases	285:361	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	5	32	theme	bowel	377:381	arg1	disorder					410:417	a chronic disorder	400:417	a chronic disorder of the human gastrointestinal tract	400:453	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	5	32	theme	bowel	377:381	arg1	IBD					392:394	IBD	392:394	IBD	392:394	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	5	32	theme	bowel	377:381	arg1	disease					383:389	Inflammatory bowel disease	364:389	Inflammatory bowel disease (IBD)	364:395	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	9	33	theme	mouse	833:837	arg1	model					839:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model was applied to explore the anti-inflammatory activity of JXR.					
34735907	17	34	theme	colitis	2096:2102	arg1	model					2110:2114	DSS colitis mouse model	2092:2114	DSS colitis mouse model	2092:2114	Furthermore, JXR could restore the microbial diversity by suppressing Bacteroidaceae, increasing Bifidobacteriales and Melainabacteria in DSS colitis mouse model.					
34735907	19	35	theme	colon	2527:2531	arg1	health					2533:2538	colon health	2527:2538	colon health	2527:2538	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	1	36	theme	signaling	96:104	arg1	pathways					106:113	MAPK signaling pathways	91:113	MAPK signaling pathways	91:113	Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice.					
34735907	4	37	theme	fever	306:310	arg1	therapy					289:295	the therapy	285:295	the therapy of cold, fever, diarrhea, digestive disorders, and other diseases	285:361	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	15	38	theme	cytokines	1823:1831	arg1	secretion					1772:1780	the secretion	1768:1780	the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β)	1768:1852	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	11	39	dep	cytokines	1085:1093	arg1	interleukin-1β					1154:1167	interleukin-1β (IL-1β)	1154:1175	interleukin-1β (IL-1β)	1154:1175	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	39	dep	cytokines	1085:1093	arg1	interleukin-6					1129:1141	interleukin-6	1129:1141	interleukin-6 (IL-6)	1129:1148	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	39	dep	cytokines	1085:1093	arg1	factor-α					1111:1118	tumor necrosis factor-α	1096:1118	tumor necrosis factor-α (TNF-α)	1096:1126	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	39	dep	cytokines	1085:1093	arg1	IL-6					1144:1147	IL-6	1144:1147	IL-6	1144:1147	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	39	dep	cytokines	1085:1093	arg1	cytokines					1085:1093	cytokines	1085:1093	cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β))	1085:1176	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	39	dep	cytokines	1085:1093	arg1	TNF-α					1121:1125	TNF-α	1121:1125	TNF-α	1121:1125	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	0	40	theme	Bioactive	0:8	arg1	constituents					10:21	Bioactive constituents	0:21	Bioactive constituents of Mosla chinensis-cv	0:43	Bioactive constituents of Mosla chinensis-cv.					
34735907	15	41	dep	reduced	1652:1658	arg1	increased					1709:1717	increased	1709:1717	increased the activity of antioxidative enzyme	1709:1754	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	41	dep	reduced	1652:1658	arg1	suppressed					1757:1766	suppressed	1757:1766	suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β)	1757:1852	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	4	42	used	used	276:279	arg2	JXR					229:231	JXR	229:231	JXR	229:231	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	4	42	used	used	276:279	arg2	Jiangxiangru					215:226	Jiangxiangru	215:226	Jiangxiangru (JXR)	215:232	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	4	42	used	used	276:279	arg2	medicine					257:264	a traditional Chinese medicine	235:264	a traditional Chinese medicine	235:264	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	6	43	theme	JXR	485:487	arg1	effect					475:480	the effect	471:480	the effect of JXR on IBD	471:494	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	6	43	theme	JXR	485:487	arg1	composition					522:532	the active ingredient composition	500:532	the active ingredient composition of JXR	500:539	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	10	44	theme	tissue	975:980	arg1	weight					916:921	body weight	911:921	body weight	911:921	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	10	44	theme	tissue	975:980	arg1	length					930:935	colon length	924:935	colon length	924:935	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	10	44	theme	tissue	975:980	arg1	status					959:964	histopathological status	941:964	histopathological status	941:964	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	19	45	theme	therapeutic	2348:2358	arg1	potential					2360:2368	therapeutic potential	2348:2368	therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties	2348:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	10	46	theme	colon	924:928	arg1	length					930:935	colon length	924:935	colon length	924:935	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	2	47	dep	BACKGROUND	177:186	arg1	Maxim					204:208	Maxim	204:208	Maxim	204:208	BACKGROUND Mosla chinensis Maxim.					
34735907	0	48	theme	chinensis-cv	32:43	arg1	constituents					10:21	Bioactive constituents	0:21	Bioactive constituents of Mosla chinensis-cv	0:43	Bioactive constituents of Mosla chinensis-cv.					
34735907	10	49	theme	colon	969:973	arg1	tissue					975:980	colon tissue	969:980	colon tissue	969:980	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	11	50	theme	corresponding	1179:1191	arg1	mRNA					1193:1196	corresponding mRNA	1179:1196	corresponding mRNA	1179:1196	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	4	51	theme	other	348:352	arg1	diseases					354:361	other diseases	348:361	other diseases	348:361	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	18	52	theme	gut	2279:2281	arg1	microbiota					2283:2292	gut microbiota	2279:2292	gut microbiota	2279:2292	CONCLUSIONS This study demonstrated that JXR composed with various bioactive compounds, effectively ameliorated colitis, restored the redox balance and regulated gut microbiota.					
34735907	9	53	theme	JXR	902:904	arg1	activity					890:897	the anti-inflammatory activity	868:897	the anti-inflammatory activity of JXR	868:904	A DSS induced colitis mouse model was applied to explore the anti-inflammatory activity of JXR.					
34735907	4	54	theme	diarrhea	313:320	arg1	therapy					289:295	the therapy	285:295	the therapy of cold, fever, diarrhea, digestive disorders, and other diseases	285:361	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	5	55	theme	gastrointestinal	432:447	arg1	tract					449:453	the human gastrointestinal tract	422:453	the human gastrointestinal tract	422:453	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	11	56	theme	necrosis	1102:1109	arg1	cytokines					1085:1093	cytokines	1085:1093	cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β))	1085:1176	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	56	theme	necrosis	1102:1109	arg1	TNF-α					1121:1125	TNF-α	1121:1125	TNF-α	1121:1125	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	56	theme	necrosis	1102:1109	arg1	factor-α					1111:1118	tumor necrosis factor-α	1096:1118	tumor necrosis factor-α (TNF-α)	1096:1126	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	4	57	theme	digestive	323:331	arg1	disorders					333:341	digestive disorders	323:341	digestive disorders	323:341	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	18	58	theme	various	2176:2182	arg1	compounds					2194:2202	various bioactive compounds	2176:2202	various bioactive compounds	2176:2202	CONCLUSIONS This study demonstrated that JXR composed with various bioactive compounds, effectively ameliorated colitis, restored the redox balance and regulated gut microbiota.					
34735907	6	59	theme	ingredient	511:520	arg1	composition					522:532	the active ingredient composition	500:532	the active ingredient composition of JXR	500:539	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	16	60	theme	signaling	1935:1943	arg1	pathway					1945:1951	mitogen-activated protein kinases (MAPKs) signaling pathway	1893:1951	mitogen-activated protein kinases (MAPKs) signaling pathway	1893:1951	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	15	61	theme	enzyme	1749:1754	arg1	activity					1723:1730	the activity	1719:1730	the activity of antioxidative enzyme	1719:1754	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	18	62	dep	CONCLUSIONS	2117:2127	arg1	demonstrated					2140:2151	demonstrated	2140:2151	demonstrated that JXR composed with various bioactive compounds, effectively ameliorated colitis, restored the redox balance and regulated gut microbiota.	2140:2293	CONCLUSIONS This study demonstrated that JXR composed with various bioactive compounds, effectively ameliorated colitis, restored the redox balance and regulated gut microbiota.					
34735907	15	63	theme	JXR	1624:1626	arg1	treatment					1628:1636	JXR treatment	1624:1636	JXR treatment	1624:1636	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	6	64	theme	JXR	537:539	arg1	effect					475:480	the effect	471:480	the effect of JXR on IBD	471:494	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	6	64	theme	JXR	537:539	arg1	composition					522:532	the active ingredient composition	500:532	the active ingredient composition of JXR	500:539	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	7	65	theme	possible	686:693	arg1	mechanisms					713:722	the possible anti-inflammatory mechanisms	682:722	the possible anti-inflammatory mechanisms	682:722	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	19	66	from	study	2320:2324	arg1	Results					2295:2301	Results	2295:2301	Results from the present study	2295:2324	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	14	67	dep	ameliorated	1560:1570	arg1	restore					1586:1592	restore	1586:1592	restore the redox balance in the gut	1586:1621	The results of DSS-induced colitis mice model indicated that JXR effectively ameliorated inflammation, restore the redox balance in the gut.					
34735907	6	68	from	effect	475:480	arg1	IBD					492:494	IBD	492:494	IBD	492:494	Research about the effect of JXR on IBD and the active ingredient composition of JXR remains deficiency.					
34735907	19	69	from	IBD	2380:2382	arg1	potential					2360:2368	therapeutic potential	2348:2368	therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties	2348:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	14	70	theme	model	1523:1527	arg1	results					1487:1493	The results	1483:1493	The results of DSS-induced colitis mice model	1483:1527	The results of DSS-induced colitis mice model indicated that JXR effectively ameliorated inflammation, restore the redox balance in the gut.					
34735907	15	71	theme	inflammatory	1785:1796	arg1	mediators					1798:1806	inflammatory mediators	1785:1806	inflammatory mediators (NO, PGE2)	1785:1817	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	19	72	theme	scientific	2458:2467	arg1	basis					2469:2473	a scientific basis	2456:2473	a scientific basis for using JXR as a functional ingredient to promote colon health	2456:2538	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	13	73	theme	main	1468:1471	arg1	acid					1450:1453	rosmarinic acid	1439:1453	rosmarinic acid (RA)	1439:1458	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	13	73	theme	main	1468:1471	arg1	compound					1473:1480	the main compound	1464:1480	the main compound	1464:1480	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	11	74	theme	inflammatory	1013:1024	arg1	mediators					1026:1034	inflammatory mediators	1013:1034	inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2))	1013:1079	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	18	75	theme	redox	2251:2255	arg1	balance					2257:2263	the redox balance	2247:2263	the redox balance	2247:2263	CONCLUSIONS This study demonstrated that JXR composed with various bioactive compounds, effectively ameliorated colitis, restored the redox balance and regulated gut microbiota.					
34735907	14	76	theme	colitis	1510:1516	arg1	model					1523:1527	DSS-induced colitis mice model	1498:1527	DSS-induced colitis mice model	1498:1527	The results of DSS-induced colitis mice model indicated that JXR effectively ameliorated inflammation, restore the redox balance in the gut.					
34735907	15	77	theme	reactive	1678:1685	arg1	ROS					1703:1705	ROS	1703:1705	ROS	1703:1705	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	77	theme	reactive	1678:1685	arg1	species					1694:1700	reactive oxygen species	1678:1700	reactive oxygen species (ROS)	1678:1706	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	8	78	theme	bioactive	737:745	arg1	profile					747:753	The bioactive profile	733:753	The bioactive profile of JXR extracts	733:769	METHODS The bioactive profile of JXR extracts was determined by UPLC-LTQ-Orbitrap-MS.					
34735907	1	79	theme	DSS-induced	151:161	arg1	colitis					163:169	DSS-induced colitis	151:169	DSS-induced colitis mice	151:174	Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice.					
34735907	4	80	theme	Chinese	249:255	arg1	medicine					257:264	a traditional Chinese medicine	235:264	a traditional Chinese medicine	235:264	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	4	80	theme	Chinese	249:255	arg1	Jiangxiangru					215:226	Jiangxiangru	215:226	Jiangxiangru (JXR)	215:232	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	12	81	theme	gut	1266:1268	arg1	composition					1280:1290	gut microbial composition	1266:1290	gut microbial composition	1266:1290	Oxidation pressure and gut microbial composition were also explored.					
34735907	8	82	theme	extracts	762:769	arg1	profile					747:753	The bioactive profile	733:753	The bioactive profile of JXR extracts	733:769	METHODS The bioactive profile of JXR extracts was determined by UPLC-LTQ-Orbitrap-MS.					
34735907	7	83	theme	anti-inflammatory	636:652	arg1	property					654:661	the anti-inflammatory property	632:661	the anti-inflammatory property of JXR	632:668	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	9	84	theme	DSS	813:815	arg1	model					839:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model was applied to explore the anti-inflammatory activity of JXR.					
34735907	9	85	theme	colitis	825:831	arg1	model					839:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model	811:843	A DSS induced colitis mouse model was applied to explore the anti-inflammatory activity of JXR.					
34735907	5	86	theme	Inflammatory	364:375	arg1	disorder					410:417	a chronic disorder	400:417	a chronic disorder of the human gastrointestinal tract	400:453	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	5	86	theme	Inflammatory	364:375	arg1	IBD					392:394	IBD	392:394	IBD	392:394	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	5	86	theme	Inflammatory	364:375	arg1	disease					383:389	Inflammatory bowel disease	364:389	Inflammatory bowel disease (IBD)	364:395	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	19	87	dep	provide	2326:2332	arg1	provide					2448:2454	provide	2448:2454	provide a scientific basis for using JXR as a functional ingredient to promote colon health	2448:2538	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	11	88	theme	protein	1202:1208	arg1	levels					1221:1226	protein expression levels	1202:1226	protein expression levels	1202:1226	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	17	89	theme	DSS	2092:2094	arg1	model					2110:2114	DSS colitis mouse model	2092:2114	DSS colitis mouse model	2092:2114	Furthermore, JXR could restore the microbial diversity by suppressing Bacteroidaceae, increasing Bifidobacteriales and Melainabacteria in DSS colitis mouse model.					
34735907	7	90	theme	phytochemical	602:614	arg1	composition					616:626	the phytochemical composition	598:626	the phytochemical composition	598:626	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	1	91	theme	MAPK	91:94	arg1	pathways					106:113	MAPK signaling pathways	91:113	MAPK signaling pathways	91:113	Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice.					
34735907	19	92	from	potential	2360:2368	arg1	IBD					2380:2382	IBD	2380:2382	IBD based on its anti-inflammatory and antioxidant properties	2380:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	17	93	theme	mouse	2104:2108	arg1	model					2110:2114	DSS colitis mouse model	2092:2114	DSS colitis mouse model	2092:2114	Furthermore, JXR could restore the microbial diversity by suppressing Bacteroidaceae, increasing Bifidobacteriales and Melainabacteria in DSS colitis mouse model.					
34735907	10	94	theme	histopathological	941:957	arg1	status					959:964	histopathological status	941:964	histopathological status	941:964	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	12	95	theme	Oxidation	1243:1251	arg1	pressure					1253:1260	Oxidation pressure	1243:1260	Oxidation pressure	1243:1260	Oxidation pressure and gut microbial composition were also explored.					
34735907	19	96	from	JXR	2373:2375	arg1	IBD					2380:2382	IBD	2380:2382	IBD based on its anti-inflammatory and antioxidant properties	2380:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	13	97	theme	rosmarinic	1439:1448	arg1	RA					1456:1457	RA	1456:1457	RA	1456:1457	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	13	97	theme	rosmarinic	1439:1448	arg1	acid					1450:1453	rosmarinic acid	1439:1453	rosmarinic acid (RA)	1439:1458	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	13	97	theme	rosmarinic	1439:1448	arg1	compound					1473:1480	the main compound	1464:1480	the main compound	1464:1480	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	0	98	theme	Mosla	26:30	arg1	chinensis-cv					32:43	Mosla chinensis-cv	26:43	Mosla chinensis-cv	26:43	Bioactive constituents of Mosla chinensis-cv.					
34735907	19	99	theme	JXR	2373:2375	arg1	potential					2360:2368	therapeutic potential	2348:2368	therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties	2348:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	16	100	theme	mitogen-activated	1893:1909	arg1	MAPKs					1928:1932	MAPKs	1928:1932	MAPKs	1928:1932	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	16	100	theme	mitogen-activated	1893:1909	arg1	kinases					1919:1925	mitogen-activated protein kinases	1893:1925	mitogen-activated protein kinases (MAPKs) signaling pathway	1893:1951	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	10	101	theme	body	911:914	arg1	weight					916:921	body weight	911:921	body weight	911:921	The body weight, colon length and histopathological status of colon tissue were evaluated.					
34735907	15	102	theme	species	1694:1700	arg1	production					1664:1673	the production	1660:1673	the production of reactive oxygen species (ROS)	1660:1706	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	103	dep	cytokines	1823:1831	arg1	TNF-α					1834:1838	TNF-α	1834:1838	TNF-α	1834:1838	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	103	dep	cytokines	1823:1831	arg1	IL-1β					1847:1851	IL-1β	1847:1851	IL-1β	1847:1851	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	15	103	dep	cytokines	1823:1831	arg1	IL-6					1841:1844	IL-6	1841:1844	IL-6	1841:1844	JXR treatment significantly reduced the production of reactive oxygen species (ROS), increased the activity of antioxidative enzyme, suppressed the secretion of inflammatory mediators (NO, PGE2) and cytokines (TNF-α, IL-6, IL-1β).					
34735907	19	104	theme	potential	2360:2368	arg1	insight					2337:2343	an insight	2334:2343	an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties	2334:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	11	105	theme	cytokines	1085:1093	arg1	content					1002:1008	The content	998:1008	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β))	998:1176	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	105	theme	cytokines	1085:1093	arg1	levels					1221:1226	protein expression levels	1202:1226	protein expression levels	1202:1226	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	105	theme	cytokines	1085:1093	arg1	mRNA					1193:1196	corresponding mRNA	1179:1196	corresponding mRNA	1179:1196	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	9	106	theme	anti-inflammatory	872:888	arg1	activity					890:897	the anti-inflammatory activity	868:897	the anti-inflammatory activity of JXR	868:904	A DSS induced colitis mouse model was applied to explore the anti-inflammatory activity of JXR.					
34735907	13	107	theme	large	1423:1427	arg1	part					1429:1432	a large part	1421:1432	a large part	1421:1432	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	8	108	dep	METHODS	725:731	arg1	determined					775:784	determined	775:784	was determined by UPLC-LTQ-Orbitrap-MS	771:808	METHODS The bioactive profile of JXR extracts was determined by UPLC-LTQ-Orbitrap-MS.					
34735907	4	109	theme	disorders	333:341	arg1	therapy					289:295	the therapy	285:295	the therapy of cold, fever, diarrhea, digestive disorders, and other diseases	285:361	Jiangxiangru (JXR), a traditional Chinese medicine, commonly used for the therapy of cold, fever, diarrhea, digestive disorders, and other diseases.					
34735907	7	110	dep	PURPOSE	561:567	arg1	aims					580:583	aims	580:583	aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms	580:722	PURPOSE This study aims to determine the phytochemical composition and the anti-inflammatory property of JXR, as well as the possible anti-inflammatory mechanisms.					
34735907	13	111	theme	phenolic	1381:1388	arg1	acids					1390:1394	phenolic acids	1381:1394	phenolic acids	1381:1394	RESULTS Totally 63 constitutes were identified from JXR, among them, phenolic acids and flavonoids comprised a large part, and rosmarinic acid (RA) was the main compound.					
34735907	16	112	theme	pathway	1945:1951	arg1	activation					1879:1888	the activation	1875:1888	the activation of mitogen-activated protein kinases (MAPKs) signaling pathway	1875:1951	JXR also restrained the activation of mitogen-activated protein kinases (MAPKs) signaling pathway.					
34735907	5	113	theme	human	426:430	arg1	tract					449:453	the human gastrointestinal tract	422:453	the human gastrointestinal tract	422:453	Inflammatory bowel disease (IBD) is a chronic disorder of the human gastrointestinal tract.					
34735907	19	114	theme	antioxidant	2419:2429	arg1	properties					2431:2440	its anti-inflammatory and antioxidant properties	2393:2440	its anti-inflammatory and antioxidant properties	2393:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
34735907	11	115	theme	tumor	1096:1100	arg1	cytokines					1085:1093	cytokines	1085:1093	cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β))	1085:1176	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	115	theme	tumor	1096:1100	arg1	TNF-α					1121:1125	TNF-α	1121:1125	TNF-α	1121:1125	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	11	115	theme	tumor	1096:1100	arg1	factor-α					1111:1118	tumor necrosis factor-α	1096:1118	tumor necrosis factor-α (TNF-α)	1096:1126	The content of inflammatory mediators (nitric oxide (NO), prostaglandin E2 (PGE2)) and cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)), corresponding mRNA and protein expression levels were analyzed.					
34735907	18	116	theme	bioactive	2184:2192	arg1	compounds					2194:2202	various bioactive compounds	2176:2202	various bioactive compounds	2176:2202	CONCLUSIONS This study demonstrated that JXR composed with various bioactive compounds, effectively ameliorated colitis, restored the redox balance and regulated gut microbiota.					
34735907	19	117	theme	anti-inflammatory	2397:2413	arg1	properties					2431:2440	its anti-inflammatory and antioxidant properties	2393:2440	its anti-inflammatory and antioxidant properties	2393:2440	Results from the present study provide an insight of therapeutic potential of JXR in IBD based on its anti-inflammatory and antioxidant properties, also provide a scientific basis for using JXR as a functional ingredient to promote colon health.					
32512103	1	0	theme	TEMPO-oxidized	177:190	arg1	NCF					215:217	NCF	215:217	NCF	215:217	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	0	theme	TEMPO-oxidized	177:190	arg1	fibrils					206:212	TEMPO-oxidized nanocellulose fibrils	177:212	TEMPO-oxidized nanocellulose fibrils (NCF)	177:218	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	6	1	theme	synergistic	822:832	arg1	effect					834:839	This synergistic effect	817:839	This synergistic effect	817:839	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	3	2	theme	composite	550:558	arg1	film					560:563	the PVA/NCF/GO composite film	535:563	the PVA/NCF/GO composite film	535:563	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	1	3	theme	nanocellulose	192:204	arg1	NCF					215:217	NCF	215:217	NCF	215:217	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	3	theme	nanocellulose	192:204	arg1	fibrils					206:212	TEMPO-oxidized nanocellulose fibrils	177:212	TEMPO-oxidized nanocellulose fibrils (NCF)	177:218	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	7	4	theme	multifunctional	1099:1113	arg1	nanocomposites					1115:1128	novel multifunctional nanocomposites	1093:1128	novel multifunctional nanocomposites	1093:1128	The synergistic effect between the fillers provides a new idea for the preparation of novel multifunctional nanocomposites.					
32512103	6	5	theme	nanofillers	924:934	arg1	agglomeration					907:919	the agglomeration	903:919	the agglomeration of nanofillers in the polymer matrix	903:956	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	3	6	theme	film	560:563	arg1	higher					569:574	higher	569:574	higher	569:574	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	3	6	theme	film	560:563	arg1	performance					520:530	the UV-shielding performance	503:530	the UV-shielding performance of the PVA/NCF/GO composite film	503:563	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	1	7	used	used	332:335	arg2	1					313:313	1	313:313	1	313:313	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	7	used	used	332:335	arg2	1					324:324	1	324:324	1	324:324	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	7	used	used	332:335	arg2	1					307:307	1	307:307	1	307:307	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	8	theme	different	262:270	arg1	ratios					277:282	different mass ratios	262:282	different mass ratios of NCF	262:289	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	4	9	theme	tensile	590:596	arg1	strength					598:605	The tensile strength	586:605	The tensile strength	586:605	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	6	10	from	agglomeration	907:919	arg1	matrix					951:956	the polymer matrix	939:956	the polymer matrix	939:956	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	4	11	theme	Young	611:615	arg1	modulus					619:625	Young's modulus	611:625	Young's modulus	611:625	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	1	12	theme	mass	272:275	arg1	ratios					277:282	different mass ratios	262:282	different mass ratios of NCF	262:289	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	2	13	theme	hybrid	386:391	arg1	NCF					342:344	The NCF	338:344	The NCF	338:344	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	2	13	theme	hybrid	386:391	arg1	filler					393:398	a hybrid filler	384:398	a hybrid filler	384:398	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	0	14	from	nanofiller	55:64	arg1	nanocomposites					73:86	PVA nanocomposites	69:86	PVA nanocomposites	69:86	Effects of cellulose nanofibrils/graphene oxide hybrid nanofiller in PVA nanocomposites.					
32512103	6	15	theme	hybrid	873:878	arg1	nanofiller					880:889	the hybrid nanofiller	869:889	the hybrid nanofiller	869:889	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	5	16	theme	nanocomposites	787:800	arg1	stabilities					768:778	the thermal stabilities	756:778	the thermal stabilities of the nanocomposites	756:800	Moreover, the thermal stabilities of the nanocomposites also improved.					
32512103	1	17	theme	NCF/GO	89:94	arg1	nanofillers					103:113	NCF/GO hybrid nanofillers	89:113	NCF/GO hybrid nanofillers with excellent UV-shielding properties	89:152	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	18	theme	hybrid	96:101	arg1	nanofillers					103:113	NCF/GO hybrid nanofillers	89:113	NCF/GO hybrid nanofillers with excellent UV-shielding properties	89:152	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	4	19	theme	water	711:715	arg1	absorption					717:726	the water absorption	707:726	the water absorption	707:726	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	7	20	theme	synergistic	1011:1021	arg1	effect					1023:1028	The synergistic effect	1007:1028	The synergistic effect between the fillers	1007:1048	The synergistic effect between the fillers provides a new idea for the preparation of novel multifunctional nanocomposites.					
32512103	3	21	theme	%	482:482	arg1	nanofiller					491:500	5% hybrid nanofiller	481:500	5% hybrid nanofiller	481:500	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	6	22	gly	homogeneity	976:986	arg1	dispersion					995:1004	the dispersion	991:1004	the dispersion	991:1004	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	5	23	theme	thermal	760:766	arg1	stabilities					768:778	the thermal stabilities	756:778	the thermal stabilities of the nanocomposites	756:800	Moreover, the thermal stabilities of the nanocomposites also improved.					
32512103	6	24	theme	nanofiller	880:889	arg1	performance					854:864	the performance	850:864	the performance of the hybrid nanofiller	850:889	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	1	25	with	nanofillers	103:113	arg1	properties					143:152	excellent UV-shielding properties	120:152	excellent UV-shielding properties	120:152	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	26	theme	NCF	287:289	arg1	ratios					277:282	different mass ratios	262:282	different mass ratios of NCF	262:289	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	2	27	theme	synergistic	413:423	arg1	effects					425:431	the synergistic effects	409:431	the synergistic effects on polyvinyl alcohol (PVA) nanocomposites	409:473	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	1	28	theme	excellent	120:128	arg1	properties					143:152	excellent UV-shielding properties	120:152	excellent UV-shielding properties	120:152	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	1	29	theme	graphene	224:231	arg1	oxide					233:237	graphene oxide	224:237	graphene oxide (GO)	224:242	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	0	30	theme	hybrid	48:53	arg1	nanofiller					55:64	oxide hybrid nanofiller	42:64	oxide hybrid nanofiller in PVA nanocomposites	42:86	Effects of cellulose nanofibrils/graphene oxide hybrid nanofiller in PVA nanocomposites.					
32512103	3	31	theme	UV-shielding	507:518	arg1	higher					569:574	higher	569:574	higher	569:574	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	3	31	theme	UV-shielding	507:518	arg1	performance					520:530	the UV-shielding performance	503:530	the UV-shielding performance of the PVA/NCF/GO composite film	503:563	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	4	32	theme	composite	643:651	arg1	film					653:656	the PVA/CG-2 composite film	630:656	the PVA/CG-2 composite film	630:656	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	0	33	theme	oxide	42:46	arg1	nanofiller					55:64	oxide hybrid nanofiller	42:64	oxide hybrid nanofiller in PVA nanocomposites	42:86	Effects of cellulose nanofibrils/graphene oxide hybrid nanofiller in PVA nanocomposites.					
32512103	1	34	theme	UV-shielding	130:141	arg1	properties					143:152	excellent UV-shielding properties	120:152	excellent UV-shielding properties	120:152	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	2	35	theme	alcohol	446:452	arg1	nanocomposites					460:473	polyvinyl alcohol (PVA) nanocomposites	436:473	polyvinyl alcohol (PVA) nanocomposites	436:473	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	7	36	theme	nanocomposites	1115:1128	arg1	preparation					1078:1088	the preparation	1074:1088	the preparation of novel multifunctional nanocomposites	1074:1128	The synergistic effect between the fillers provides a new idea for the preparation of novel multifunctional nanocomposites.					
32512103	3	37	theme	5	481:481	arg1	%					482:482	%	482:482	%	482:482	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	2	38	theme	polyvinyl	436:444	arg1	PVA					455:457	PVA	455:457	PVA	455:457	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	2	38	theme	polyvinyl	436:444	arg1	alcohol					446:452	polyvinyl alcohol	436:452	polyvinyl alcohol (PVA) nanocomposites	436:473	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	3	39	theme	PVA/NCF/GO	539:548	arg1	film					560:563	the PVA/NCF/GO composite film	535:563	the PVA/NCF/GO composite film	535:563	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	1	40	dep	oxide	233:237	arg1	GO					240:241	GO	240:241	GO	240:241	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
32512103	7	41	theme	novel	1093:1097	arg1	nanocomposites					1115:1128	novel multifunctional nanocomposites	1093:1128	novel multifunctional nanocomposites	1093:1128	The synergistic effect between the fillers provides a new idea for the preparation of novel multifunctional nanocomposites.					
32512103	6	42	theme	dispersion	995:1004	arg1	homogeneity					976:986	the homogeneity	972:986	the homogeneity of the dispersion	972:1004	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	2	43	used	used	376:379	arg2	filler					393:398	a hybrid filler	384:398	a hybrid filler	384:398	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	2	43	used	used	376:379	arg2	NCF					342:344	The NCF	338:344	The NCF	338:344	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	3	44	theme	hybrid	484:489	arg1	nanofiller					491:500	5% hybrid nanofiller	481:500	5% hybrid nanofiller	481:500	With 5% hybrid nanofiller, the UV-shielding performance of the PVA/NCF/GO composite film was higher than 90%.					
32512103	6	45	theme	polymer	943:949	arg1	matrix					951:956	the polymer matrix	939:956	the polymer matrix	939:956	This synergistic effect improved the performance of the hybrid nanofiller by avoiding the agglomeration of nanofillers in the polymer matrix and improving the homogeneity of the dispersion.					
32512103	4	46	theme	PVA/CG-2	634:641	arg1	film					653:656	the PVA/CG-2 composite film	630:656	the PVA/CG-2 composite film	630:656	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	7	47	theme	new	1061:1063	arg1	idea					1065:1068	a new idea	1059:1068	a new idea for the preparation of novel multifunctional nanocomposites	1059:1128	The synergistic effect between the fillers provides a new idea for the preparation of novel multifunctional nanocomposites.					
32512103	4	48	theme	film	653:656	arg1	strength					598:605	The tensile strength	586:605	The tensile strength	586:605	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	4	48	theme	film	653:656	arg1	modulus					619:625	Young's modulus	611:625	Young's modulus	611:625	The tensile strength and Young's modulus of the PVA/CG-2 composite film increased by 74.5% and 278.0%, respectively, and the water absorption decreased by 59%.					
32512103	2	49	from	effects	425:431	arg1	nanocomposites					460:473	polyvinyl alcohol (PVA) nanocomposites	436:473	polyvinyl alcohol (PVA) nanocomposites	436:473	The NCF and GO were then combined and used as a hybrid filler to study the synergistic effects on polyvinyl alcohol (PVA) nanocomposites.					
32512103	0	50	theme	PVA	69:71	arg1	nanocomposites					73:86	PVA nanocomposites	69:86	PVA nanocomposites	69:86	Effects of cellulose nanofibrils/graphene oxide hybrid nanofiller in PVA nanocomposites.					
32512103	0	51	theme	cellulose	11:19	arg1	Effects					0:6	Effects	0:6	Effects of cellulose	0:19	Effects of cellulose nanofibrils/graphene oxide hybrid nanofiller in PVA nanocomposites.					
32512103	1	52	theme	raw	247:249	arg1	materials					251:259	raw materials	247:259	raw materials	247:259	NCF/GO hybrid nanofillers with excellent UV-shielding properties were prepared by using TEMPO-oxidized nanocellulose fibrils (NCF) and graphene oxide (GO) as raw materials; different mass ratios of NCF to GO (2: 1, 4: 1, 8: 1, and 16: 1) were used.					
35006904	3	0	theme	treatment	310:318	arg1	methods					320:326	the various treatment methods	298:326	the various treatment methods	298:326	Among the various treatment methods, the most economical and effective is to use a hemostatic agent.					
35006904	4	1	theme	depth	460:464	arg1	wounds					437:442	wounds	437:442	wounds of any shape or depth with high compactness and excellent accumulation effect	437:520	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	8	2	theme	microsphere	1059:1069	arg1	use					1071:1073	microsphere use	1059:1073	microsphere use	1059:1073	The relationship between hemostatic pressure and hemostatic time during microsphere use was also measured.					
35006904	10	3	theme	powder	1337:1342	arg1	compound					1344:1351	a microporous polysaccharide powder compound	1308:1351	a microporous polysaccharide powder compound	1308:1351	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	4	4	used	used	428:431	arg2	agent					415:419	A powdered hemostatic agent	393:419	A powdered hemostatic agent	393:419	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	6	5	theme	scanning	867:874	arg1	microscopy					885:894	scanning electron microscopy	867:894	scanning electron microscopy	867:894	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	9	6	theme	spleen	1143:1148	arg1	model					1179:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	The hemostatic effect was analyzed with a liver, spleen, and femoral artery bleeding model.					
35006904	9	7	theme	artery	1163:1168	arg1	model					1179:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	The hemostatic effect was analyzed with a liver, spleen, and femoral artery bleeding model.					
35006904	4	8	theme	high	471:474	arg1	compactness					476:486	high compactness	471:486	high compactness	471:486	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	8	9	theme	hemostatic	1012:1021	arg1	pressure					1023:1030	hemostatic pressure	1012:1030	hemostatic pressure	1012:1030	The relationship between hemostatic pressure and hemostatic time during microsphere use was also measured.					
35006904	5	10	theme	polymer	565:571	arg1	poly					573:576	the natural, hydrophilic polymer poly	540:576	the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA)	540:601	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	10	theme	polymer	565:571	arg1	acid					589:592	γ-glutamic acid	578:592	γ-glutamic acid	578:592	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	10	theme	polymer	565:571	arg1	γ-PGA					596:600	γ-PGA	596:600	γ-PGA	596:600	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	11	11	theme	high	1448:1451	arg1	safety					1453:1458	high safety	1448:1458	high safety	1448:1458	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	10	12	theme	hemostatic	1262:1271	arg1	effects					1273:1279	better hemostatic effects	1255:1279	better hemostatic effects	1255:1279	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	11	13	theme	hemostatic	1429:1438	arg1	effect					1440:1445	good hemostatic effect	1424:1445	good hemostatic effect	1424:1445	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	5	14	theme	water	706:710	arg1	absorptivity					712:723	water absorptivity	706:723	water absorptivity	706:723	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	15	theme	γ-glutamic	578:587	arg1	poly					573:576	the natural, hydrophilic polymer poly	540:576	the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA)	540:601	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	15	theme	γ-glutamic	578:587	arg1	acid					589:592	γ-glutamic acid	578:592	γ-glutamic acid	578:592	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	9	16	theme	femoral	1155:1161	arg1	artery					1163:1168	femoral artery	1155:1168	femoral artery	1155:1168	The hemostatic effect was analyzed with a liver, spleen, and femoral artery bleeding model.					
35006904	10	17	theme	better	1255:1260	arg1	effects					1273:1279	better hemostatic effects	1255:1279	better hemostatic effects	1255:1279	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	0	18	theme	Rapid	95:99	arg1	Hemostasis					101:110	Rapid Hemostasis	95:110	Rapid Hemostasis	95:110	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	9	19	theme	bleeding	1170:1177	arg1	model					1179:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	The hemostatic effect was analyzed with a liver, spleen, and femoral artery bleeding model.					
35006904	7	20	theme	overall	901:907	arg1	safety					909:914	The overall safety	897:914	The overall safety	897:914	The overall safety, hemolysis, pyrogenic, and intradermal irritation tests were examined.					
35006904	11	21	theme	SA/γ-PGA	1383:1390	arg1	materials					1409:1417	materials	1409:1417	materials with good hemostatic effect, high safety, and great potential in clinical applications	1409:1504	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	21	theme	SA/γ-PGA	1383:1390	arg1	microspheres					1392:1403	SA/γ-PGA microspheres	1383:1403	SA/γ-PGA microspheres	1383:1403	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	22	theme	good	1424:1427	arg1	effect					1440:1445	good hemostatic effect	1424:1445	good hemostatic effect	1424:1445	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	23	from	potential	1471:1479	arg1	applications					1493:1504	clinical applications	1484:1504	clinical applications	1484:1504	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	4	24	theme	shape	451:455	arg1	wounds					437:442	wounds	437:442	wounds of any shape or depth with high compactness and excellent accumulation effect	437:520	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	5	25	theme	good	683:686	arg1	biocompatibility					688:703	good biocompatibility	683:703	good biocompatibility	683:703	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	26	theme	hemostatic	621:630	arg1	microspheres					632:643	composite hemostatic microspheres	611:643	composite hemostatic microspheres	611:643	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	8	27	theme	hemostatic	1036:1045	arg1	time					1047:1050	hemostatic time	1036:1050	hemostatic time	1036:1050	The relationship between hemostatic pressure and hemostatic time during microsphere use was also measured.					
35006904	5	28	theme	natural	544:550	arg1	poly					573:576	the natural, hydrophilic polymer poly	540:576	the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA)	540:601	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	28	theme	natural	544:550	arg1	acid					589:592	γ-glutamic acid	578:592	γ-glutamic acid	578:592	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	28	theme	natural	544:550	arg1	γ-PGA					596:600	γ-PGA	596:600	γ-PGA	596:600	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	4	29	with	accumulation	502:513	arg1	compactness					476:486	high compactness	471:486	high compactness	471:486	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	4	30	dep	shape	451:455	arg1	effect					515:520	effect	515:520	effect	515:520	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	4	30	dep	shape	451:455	arg1	any					447:449	any	447:449	any	447:449	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	5	31	theme	sodium	650:655	arg1	alginate					657:664	sodium alginate	650:664	sodium alginate (SA)	650:669	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	5	31	theme	sodium	650:655	arg1	SA					667:668	SA	667:668	SA	667:668	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	11	32	with	materials	1409:1417	arg1	effect					1440:1445	good hemostatic effect	1424:1445	good hemostatic effect	1424:1445	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	32	with	materials	1409:1417	arg1	safety					1453:1458	high safety	1448:1458	high safety	1448:1458	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	32	with	materials	1409:1417	arg1	potential					1471:1479	great potential	1465:1479	great potential	1465:1479	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	10	33	theme	animal	1284:1289	arg1	experiments					1291:1301	animal experiments	1284:1301	animal experiments	1284:1301	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	0	34	theme	Safety	12:17	arg1	Analysis					0:7	Analysis	0:7	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid)	0:76	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	6	35	theme	hemostatic	777:786	arg1	microspheres					788:799	the hemostatic microspheres	773:799	the hemostatic microspheres	773:799	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	6	36	theme	electron	876:883	arg1	microscopy					885:894	scanning electron microscopy	867:894	scanning electron microscopy	867:894	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	6	37	dep	morphology	745:754	arg1	The					741:743	The	741:743	The	741:743	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	0	38	theme	Effectiveness	23:35	arg1	Analysis					0:7	Analysis	0:7	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid)	0:76	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	4	39	theme	excellent	492:500	arg1	accumulation					502:513	excellent accumulation	492:513	excellent accumulation	492:513	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	11	40	theme	Research	1354:1361	arg1	results					1363:1369	Research results	1354:1369	Research results	1354:1369	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	41	theme	great	1465:1469	arg1	potential					1471:1479	great potential	1465:1479	great potential	1465:1479	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	11	42	from	safety	1453:1458	arg1	applications					1493:1504	clinical applications	1484:1504	clinical applications	1484:1504	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	9	43	theme	hemostatic	1098:1107	arg1	effect					1109:1114	The hemostatic effect	1094:1114	The hemostatic effect	1094:1114	The hemostatic effect was analyzed with a liver, spleen, and femoral artery bleeding model.					
35006904	0	44	theme	Alginate/Poly	47:59	arg1	Safety					12:17	Safety	12:17	Safety	12:17	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	0	44	theme	Alginate/Poly	47:59	arg1	Effectiveness					23:35	Effectiveness	23:35	Effectiveness	23:35	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	5	45	theme	composite	611:619	arg1	microspheres					632:643	composite hemostatic microspheres	611:643	composite hemostatic microspheres	611:643	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
35006904	6	46	theme	microspheres	788:799	arg1	structure					760:768	structure	760:768	structure	760:768	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	6	46	theme	microspheres	788:799	arg1	morphology					745:754	morphology	745:754	morphology	745:754	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	2	47	theme	hemostatic	213:222	arg1	treatment					224:232	Timely hemostatic treatment	206:232	Timely hemostatic treatment	206:232	Timely hemostatic treatment is essential to minimize blood loss and improve survival.					
35006904	10	48	theme	microporous	1310:1320	arg1	compound					1344:1351	a microporous polysaccharide powder compound	1308:1351	a microporous polysaccharide powder compound	1308:1351	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	0	49	theme	Sodium	40:45	arg1	acid					72:75	γ-glutamic acid	61:75	γ-glutamic acid	61:75	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	0	49	theme	Sodium	40:45	arg1	Alginate/Poly					47:59	Sodium Alginate/Poly	40:59	Sodium Alginate/Poly(γ-glutamic acid)	40:76	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	3	50	theme	various	302:308	arg1	methods					320:326	the various treatment methods	298:326	the various treatment methods	298:326	Among the various treatment methods, the most economical and effective is to use a hemostatic agent.					
35006904	4	51	theme	hemostatic	404:413	arg1	agent					415:419	A powdered hemostatic agent	393:419	A powdered hemostatic agent	393:419	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	2	52	theme	Timely	206:211	arg1	treatment					224:232	Timely hemostatic treatment	206:232	Timely hemostatic treatment	206:232	Timely hemostatic treatment is essential to minimize blood loss and improve survival.					
35006904	1	53	theme	Most	113:116	arg1	deaths					130:135	Most preventable deaths	113:135	Most preventable deaths after trauma	113:148	Most preventable deaths after trauma are related to hemorrhage and occur early after injury.					
35006904	4	54	theme	accumulation	502:513	arg1	wounds					437:442	wounds	437:442	wounds of any shape or depth with high compactness and excellent accumulation effect	437:520	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	4	55	theme	powdered	395:402	arg1	agent					415:419	A powdered hemostatic agent	393:419	A powdered hemostatic agent	393:419	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	2	56	theme	blood	259:263	arg1	loss					265:268	blood loss	259:268	blood loss	259:268	Timely hemostatic treatment is essential to minimize blood loss and improve survival.					
35006904	1	57	theme	preventable	118:128	arg1	deaths					130:135	Most preventable deaths	113:135	Most preventable deaths after trauma	113:148	Most preventable deaths after trauma are related to hemorrhage and occur early after injury.					
35006904	11	58	theme	clinical	1484:1491	arg1	applications					1493:1504	clinical applications	1484:1504	clinical applications	1484:1504	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	6	59	dep	transform	831:839	arg1	infrared					841:848	infrared	841:848	transform infrared spectroscopy and scanning electron microscopy	831:894	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	10	60	theme	polysaccharide	1322:1335	arg1	compound					1344:1351	a microporous polysaccharide powder compound	1308:1351	a microporous polysaccharide powder compound	1308:1351	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	7	61	theme	intradermal	943:953	arg1	tests					966:970	intradermal irritation tests	943:970	intradermal irritation tests	943:970	The overall safety, hemolysis, pyrogenic, and intradermal irritation tests were examined.					
35006904	4	62	with	depth	460:464	arg1	compactness					476:486	high compactness	471:486	high compactness	471:486	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	10	63	theme	composite	1190:1198	arg1	microspheres					1200:1211	The composite microspheres	1186:1211	The composite microspheres	1186:1211	The composite microspheres were well tolerated in vivo and exhibited better hemostatic effects in animal experiments than a microporous polysaccharide powder compound.					
35006904	6	64	dep	Fourier	823:829	arg1	transform					831:839	transform	831:839	transform infrared spectroscopy and scanning electron microscopy	831:894	The morphology and structure of the hemostatic microspheres were determined using Fourier transform infrared spectroscopy and scanning electron microscopy.					
35006904	0	65	theme	γ-glutamic	61:70	arg1	acid					72:75	γ-glutamic acid	61:75	γ-glutamic acid	61:75	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	0	65	theme	γ-glutamic	61:70	arg1	Alginate/Poly					47:59	Sodium Alginate/Poly	40:59	Sodium Alginate/Poly(γ-glutamic acid)	40:76	Analysis of Safety and Effectiveness of Sodium Alginate/Poly(γ-glutamic acid) Microspheres for Rapid Hemostasis.					
35006904	11	66	from	effect	1440:1445	arg1	applications					1493:1504	clinical applications	1484:1504	clinical applications	1484:1504	Research results showed that SA/γ-PGA microspheres are materials with good hemostatic effect, high safety, and great potential in clinical applications.					
35006904	7	67	theme	irritation	955:964	arg1	tests					966:970	intradermal irritation tests	943:970	intradermal irritation tests	943:970	The overall safety, hemolysis, pyrogenic, and intradermal irritation tests were examined.					
35006904	3	68	theme	hemostatic	375:384	arg1	agent					386:390	a hemostatic agent	373:390	a hemostatic agent	373:390	Among the various treatment methods, the most economical and effective is to use a hemostatic agent.					
35006904	9	69	theme	liver	1136:1140	arg1	model					1179:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	a liver, spleen, and femoral artery bleeding model	1134:1183	The hemostatic effect was analyzed with a liver, spleen, and femoral artery bleeding model.					
35006904	4	70	with	shape	451:455	arg1	compactness					476:486	high compactness	471:486	high compactness	471:486	A powdered hemostatic agent can be used for wounds of any shape or depth with high compactness and excellent accumulation effect.					
35006904	5	71	dep	natural	544:550	arg1	hydrophilic					553:563	hydrophilic	553:563	hydrophilic	553:563	Herein, we chose the natural, hydrophilic polymer poly(γ-glutamic acid) (γ-PGA) to form composite hemostatic microspheres with sodium alginate (SA), which show good biocompatibility, water absorptivity, and viscosity.					
32513541	7	0	theme	encapsulated	1594:1605	arg1	islets					1607:1612	naked and encapsulated islets	1584:1612	naked and encapsulated islets	1584:1612	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	9	1	theme	encapsulation	1772:1784	arg1	side-effect					1757:1767	This side-effect	1752:1767	This side-effect of encapsulation	1752:1784	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	10	2	theme	alginate	2040:2047	arg1	capsule					2049:2055	the M-rich alginate capsule	2029:2055	the M-rich alginate capsule with both MSCs and RGD-G rich alginate	2029:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	5	3	from	alginate	1038:1045	arg1	viability					1102:1110	viability	1102:1110	viability	1102:1110	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	3	from	alginate	1038:1045	arg1	islets					1095:1100	human pancreatic islets viability and functionality	1078:1128	human pancreatic islets viability and functionality	1078:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	3	from	alginate	1038:1045	arg1	functionality					1116:1128	functionality	1116:1128	functionality	1116:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	3	from	alginate	1038:1045	arg1	bioprocessing					1050:1062	bioprocessing	1050:1062	bioprocessing	1050:1062	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	8	4	dep	RESULTS	1615:1621	arg1	induced					1675:1681	induced	1675:1681	induced a reduction in viability and functionality compared to naked islets	1675:1749	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	7	5	theme	naked	1584:1588	arg1	islets					1607:1612	naked and encapsulated islets	1584:1612	naked and encapsulated islets	1584:1612	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	7	6	theme	test	1539:1542	arg1	in vitro					1544:1551	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	10	7	theme	RGD-G	2076:2080	arg1	alginate					2087:2094	RGD-G rich alginate	2076:2094	RGD-G rich alginate	2076:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	1	8	theme	severe	287:292	arg1	events					302:307	severe adverse events	287:307	severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation	287:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	6	9	theme	empty	1255:1259	arg1	biocompatibility					1270:1285	the empty capsules biocompatibility	1251:1285	the empty capsules biocompatibility	1251:1285	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	10	theme	alginate	1345:1352	arg1	benefits					1313:1320	the benefits	1309:1320	the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro	1309:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	1	11	theme	islet	201:205	arg1	transplantation					207:221	islet transplantation	201:221	islet transplantation	201:221	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	7	12	theme	secretion	1522:1530	arg1	in vitro					1544:1551	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	10	13	theme	human	2105:2109	arg1	islets					2111:2116	human islets	2105:2116	human islets survival	2105:2125	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	6	14	theme	RGD-G	1334:1338	arg1	alginate					1345:1352	RGD-G rich alginate	1334:1352	RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets	1334:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	0	15	with	tripeptides	95:105	arg1	cells					73:77	mesenchymal stem cells	56:77	mesenchymal stem cells	56:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	7	16	theme	stimulated	1503:1512	arg1	secretion					1522:1530	stimulated insulin secretion	1503:1530	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	7	16	theme	stimulated	1503:1512	arg1	GSIS					1533:1536	GSIS	1533:1536	GSIS	1533:1536	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	0	17	theme	alginate	126:133	arg1	islets					154:159	alginate micro-encapsulated islets	126:159	alginate micro-encapsulated islets	126:159	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	1	18	theme	wider	228:232	arg1	number					234:239	a wider number	226:239	a wider number of type 1 diabetes patients	226:267	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	10	19	dep	CONCLUSIONS	1931:1941	arg1	show					1964:1967	show	1964:1967	show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro	1964:2152	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	0	20	theme	islets	154:159	arg1	supplementation					107:121	supplementation	107:121	supplementation of alginate micro-encapsulated islets	107:159	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	3	21	theme	viability	657:665	arg1	maintenance					636:646	the maintenance	632:646	the maintenance of graft viability and functionality	632:683	However, current biomaterials need to be optimized to: improve biocompatibility, guaranty the maintenance of graft viability and functionality, and prevent fibrosis overgrowth around the capsule in vivo.					
32513541	7	22	theme	confocal	1471:1478	arg1	microscopy					1480:1489	confocal microscopy	1471:1489	confocal microscopy	1471:1489	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	4	23	theme	stem	793:796	arg1	MSCs					805:808	MSCs	805:808	MSCs	805:808	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	4	23	theme	stem	793:796	arg1	cells					798:802	mesenchymal stem cells	781:802	mesenchymal stem cells (MSCs)	781:809	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	6	24	theme	human	1405:1409	arg1	islets					1422:1427	the encapsulated human pancreatic islets	1388:1427	the encapsulated human pancreatic islets	1388:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	5	25	theme	alginate	990:997	arg1	microcapsules					999:1011	reference M-rich alginate microcapsules	973:1011	reference M-rich alginate microcapsules	973:1011	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	6	26	from	benefits	1313:1320	arg1	functionality					1371:1383	functionality	1371:1383	functionality	1371:1383	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	26	from	benefits	1313:1320	arg1	islets					1422:1427	the encapsulated human pancreatic islets	1388:1427	the encapsulated human pancreatic islets	1388:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	26	from	benefits	1313:1320	arg1	viability					1357:1365	viability	1357:1365	viability	1357:1365	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	27	from	MSCs	1325:1328	arg1	functionality					1371:1383	functionality	1371:1383	functionality	1371:1383	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	27	from	MSCs	1325:1328	arg1	islets					1422:1427	the encapsulated human pancreatic islets	1388:1427	the encapsulated human pancreatic islets	1388:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	27	from	MSCs	1325:1328	arg1	viability					1357:1365	viability	1357:1365	viability	1357:1365	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	0	28	theme	mesenchymal	56:66	arg1	cells					73:77	mesenchymal stem cells	56:77	mesenchymal stem cells	56:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	3	29	theme	functionality	671:683	arg1	maintenance					636:646	the maintenance	632:646	the maintenance of graft viability and functionality	632:683	However, current biomaterials need to be optimized to: improve biocompatibility, guaranty the maintenance of graft viability and functionality, and prevent fibrosis overgrowth around the capsule in vivo.					
32513541	1	30	theme	islet	373:377	arg1	transplantation					379:393	allogenic islet transplantation	363:393	allogenic islet transplantation	363:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	5	31	theme	reference	973:981	arg1	microcapsules					999:1011	reference M-rich alginate microcapsules	973:1011	reference M-rich alginate microcapsules	973:1011	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	9	32	theme	MSCs	1897:1900	arg1	combination					1877:1887	the combination	1873:1887	the combination of both MSCs and the RGD-G rich alginate	1873:1928	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	4	33	contain	have	873:876	arg1	MSCs					805:808	MSCs	805:808	MSCs	805:808	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	4	33	contain	have	873:876	arg2	effects					893:899	cytoprotective effects	878:899	cytoprotective effects	878:899	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	4	33	contain	have	873:876	arg1	cells					798:802	mesenchymal stem cells	781:802	mesenchymal stem cells (MSCs)	781:809	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	4	33	contain	have	873:876	arg1	points					822:827	anchor points	815:827	anchor points consisting of tripeptides arg-gly-asp (RGD)	815:871	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	0	34	theme	arg-gly-asp	83:93	arg1	tripeptides					95:105	arg-gly-asp tripeptides	83:105	arg-gly-asp tripeptides	83:105	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	5	35	from	effect	949:954	arg1	viability					1102:1110	viability	1102:1110	viability	1102:1110	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	35	from	effect	949:954	arg1	islets					1095:1100	human pancreatic islets viability and functionality	1078:1128	human pancreatic islets viability and functionality	1078:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	35	from	effect	949:954	arg1	functionality					1116:1128	functionality	1116:1128	functionality	1116:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	35	from	effect	949:954	arg1	bioprocessing					1050:1062	bioprocessing	1050:1062	bioprocessing	1050:1062	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	6	36	dep	We	1139:1140	arg1	then					1190:1193	then	1190:1193	then	1190:1193	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	36	dep	We	1139:1140	arg1	characterized					1142:1154	characterized	1142:1154	characterized the microcapsules components	1142:1183	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	9	37	with	complete	1859:1866	arg1	combination					1877:1887	the combination	1873:1887	the combination of both MSCs and the RGD-G rich alginate	1873:1928	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	6	38	from	alginate	1345:1352	arg1	functionality					1371:1383	functionality	1371:1383	functionality	1371:1383	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	38	from	alginate	1345:1352	arg1	islets					1422:1427	the encapsulated human pancreatic islets	1388:1427	the encapsulated human pancreatic islets	1388:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	38	from	alginate	1345:1352	arg1	viability					1357:1365	viability	1357:1365	viability	1357:1365	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	39	theme	microcapsules	1160:1172	arg1	components					1174:1183	the microcapsules components	1156:1183	the microcapsules components	1156:1183	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	9	40	theme	rich	1916:1919	arg1	alginate					1921:1928	the RGD-G rich alginate	1906:1928	the RGD-G rich alginate	1906:1928	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	8	41	theme	M-rich	1650:1655	arg1	capsules					1666:1673	reference M-rich alginate capsules	1640:1673	reference M-rich alginate capsules	1640:1673	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	0	42	theme	Improved	0:7	arg1	islets					15:20	Improved human islets	0:20	Improved human islets' viability and functionality with mesenchymal stem cells	0:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	42	theme	Improved	0:7	arg1	functionality					37:49	functionality	37:49	functionality	37:49	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	42	theme	Improved	0:7	arg1	viability					23:31	viability	23:31	viability	23:31	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	1	43	theme	adverse	294:300	arg1	events					302:307	severe adverse events	287:307	severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation	287:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	10	44	with	capsule	2049:2055	arg1	MSCs					2067:2070	MSCs	2067:2070	MSCs	2067:2070	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	10	44	with	capsule	2049:2055	arg1	alginate					2087:2094	RGD-G rich alginate	2076:2094	RGD-G rich alginate	2076:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	4	45	theme	anchor	815:820	arg1	points					822:827	anchor points	815:827	anchor points consisting of tripeptides arg-gly-asp (RGD)	815:871	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	2	46	theme	therapy	479:485	arg1	prospects					443:451	the prospects	439:451	the prospects of immunosuppressive-free therapy	439:485	In this context, microencapsulation offers the prospects of immunosuppressive-free therapy by physically isolating islets from the immune system.					
32513541	1	47	theme	related	309:315	arg1	events					302:307	severe adverse events	287:307	severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation	287:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	5	48	theme	rich	1033:1036	arg1	alginate					1038:1045	RGD-G rich alginate	1027:1045	RGD-G rich alginate	1027:1045	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	0	49	dep	in vitro	161:168	arg1	islets					15:20	Improved human islets	0:20	Improved human islets' viability and functionality with mesenchymal stem cells	0:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	49	dep	in vitro	161:168	arg1	tripeptides					95:105	arg-gly-asp tripeptides	83:105	arg-gly-asp tripeptides	83:105	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	49	dep	in vitro	161:168	arg1	functionality					37:49	functionality	37:49	functionality	37:49	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	49	dep	in vitro	161:168	arg1	viability					23:31	viability	23:31	viability	23:31	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	4	50	theme	cytoprotective	878:891	arg1	effects					893:899	cytoprotective effects	878:899	cytoprotective effects	878:899	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	3	51	theme	fibrosis	698:705	arg1	overgrowth					707:716	fibrosis overgrowth	698:716	fibrosis overgrowth	698:716	However, current biomaterials need to be optimized to: improve biocompatibility, guaranty the maintenance of graft viability and functionality, and prevent fibrosis overgrowth around the capsule in vivo.					
32513541	0	52	with	islets	15:20	arg1	cells					73:77	mesenchymal stem cells	56:77	mesenchymal stem cells	56:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	6	53	theme	microcapsule	1207:1218	arg1	product					1230:1236	the new microcapsule composite product	1199:1236	the new microcapsule composite product	1199:1236	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	1	54	theme	type	244:247	arg1	patients					260:267	type 1 diabetes patients	244:267	type 1 diabetes patients	244:267	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	7	55	theme	islets	1607:1612	arg1	functionality					1567:1579	the functionality	1563:1579	the functionality of naked and encapsulated islets	1563:1612	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	0	56	dep	islets	15:20	arg1	islets					15:20	Improved human islets	0:20	Improved human islets' viability and functionality with mesenchymal stem cells	0:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	56	dep	islets	15:20	arg1	supplementation					107:121	supplementation	107:121	supplementation of alginate micro-encapsulated islets	107:159	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	56	dep	islets	15:20	arg1	functionality					37:49	functionality	37:49	functionality	37:49	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	56	dep	islets	15:20	arg1	viability					23:31	viability	23:31	viability	23:31	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	3	57	theme	capsule	729:735	arg1	in vivo					737:743	the capsule in vivo	725:743	the capsule in vivo	725:743	However, current biomaterials need to be optimized to: improve biocompatibility, guaranty the maintenance of graft viability and functionality, and prevent fibrosis overgrowth around the capsule in vivo.					
32513541	1	58	theme	diabetes	251:258	arg1	patients					260:267	type 1 diabetes patients	244:267	type 1 diabetes patients	244:267	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	5	59	theme	human	1078:1082	arg1	islets					1095:1100	human pancreatic islets viability and functionality	1078:1128	human pancreatic islets viability and functionality	1078:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	59	theme	human	1078:1082	arg1	functionality					1116:1128	functionality	1116:1128	functionality	1116:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	59	theme	human	1078:1082	arg1	viability					1102:1110	viability	1102:1110	viability	1102:1110	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	8	60	theme	naked	1738:1742	arg1	islets					1744:1749	naked islets	1738:1749	naked islets	1738:1749	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	8	61	from	reduction	1685:1693	arg1	viability					1698:1706	viability	1698:1706	viability	1698:1706	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	8	61	from	reduction	1685:1693	arg1	functionality					1712:1724	functionality	1712:1724	functionality	1712:1724	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	10	62	theme	M-rich	2033:2038	arg1	capsule					2049:2055	the M-rich alginate capsule	2029:2055	the M-rich alginate capsule with both MSCs and RGD-G rich alginate	2029:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	6	63	theme	capsules	1261:1268	arg1	biocompatibility					1270:1285	the empty capsules biocompatibility	1251:1285	the empty capsules biocompatibility	1251:1285	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	4	64	theme	pancreatic	904:913	arg1	islets					915:920	pancreatic islets	904:920	pancreatic islets	904:920	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	6	65	theme	rich	1340:1343	arg1	alginate					1345:1352	RGD-G rich alginate	1334:1352	RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets	1334:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	1	66	theme	transplantation	207:221	arg1	extension					188:196	The extension	184:196	The extension of islet transplantation to a wider number of type 1 diabetes patients	184:267	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	5	67	dep	islets	1095:1100	arg1	islets					1095:1100	human pancreatic islets viability and functionality	1078:1128	human pancreatic islets viability and functionality	1078:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	67	dep	islets	1095:1100	arg1	functionality					1116:1128	functionality	1116:1128	functionality	1116:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	67	dep	islets	1095:1100	arg1	viability					1102:1110	viability	1102:1110	viability	1102:1110	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	0	68	theme	micro-encapsulated	135:152	arg1	islets					154:159	alginate micro-encapsulated islets	126:159	alginate micro-encapsulated islets	126:159	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	9	69	theme	MSCs	1830:1833	arg1	presence					1818:1825	the presence	1814:1825	the presence of MSCs	1814:1833	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	7	70	theme	insulin	1514:1520	arg1	secretion					1522:1530	stimulated insulin secretion	1503:1530	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	7	70	theme	insulin	1514:1520	arg1	GSIS					1533:1536	GSIS	1533:1536	GSIS	1533:1536	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	10	71	theme	rich	2082:2085	arg1	alginate					2087:2094	RGD-G rich alginate	2076:2094	RGD-G rich alginate	2076:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	3	72	theme	graft	651:655	arg1	viability					657:665	graft viability	651:665	graft viability	651:665	However, current biomaterials need to be optimized to: improve biocompatibility, guaranty the maintenance of graft viability and functionality, and prevent fibrosis overgrowth around the capsule in vivo.					
32513541	7	73	theme	glucose	1495:1501	arg1	in vitro					1544:1551	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	glucose stimulated insulin secretion (GSIS) test in vitro	1495:1551	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	6	74	theme	pancreatic	1411:1420	arg1	islets					1422:1427	the encapsulated human pancreatic islets	1388:1427	the encapsulated human pancreatic islets	1388:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	5	75	theme	M-rich	983:988	arg1	microcapsules					999:1011	reference M-rich alginate microcapsules	973:1011	reference M-rich alginate microcapsules	973:1011	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	4	76	theme	mesenchymal	781:791	arg1	MSCs					805:808	MSCs	805:808	MSCs	805:808	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	4	76	theme	mesenchymal	781:791	arg1	cells					798:802	mesenchymal stem cells	781:802	mesenchymal stem cells (MSCs)	781:809	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	10	77	theme	islets	2111:2116	arg1	survival					2118:2125	human islets survival	2105:2125	human islets survival	2105:2125	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	6	78	theme	encapsulated	1392:1403	arg1	islets					1422:1427	the encapsulated human pancreatic islets	1388:1427	the encapsulated human pancreatic islets	1388:1427	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	0	79	theme	stem	68:71	arg1	cells					73:77	mesenchymal stem cells	56:77	mesenchymal stem cells	56:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	10	80	theme	present	1947:1953	arg1	findings					1955:1962	The present findings	1943:1962	The present findings	1943:1962	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	4	81	dep	tripeptides	843:853	arg1	RGD					868:870	RGD	868:870	RGD	868:870	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	4	81	dep	tripeptides	843:853	arg1	arg-gly-asp					855:865	arg-gly-asp	855:865	tripeptides arg-gly-asp (RGD)	843:871	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	10	82	dep	survival	2118:2125	arg1	in vitro					2145:2152	in vitro	2145:2152	in vitro	2145:2152	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	7	83	theme	viability	1452:1460	arg1	tests					1462:1466	viability tests	1452:1466	viability tests	1452:1466	We performed viability tests by confocal microscopy and glucose stimulated insulin secretion (GSIS) test in vitro to assess the functionality of naked and encapsulated islets.					
32513541	4	84	theme	Accumulating	746:757	arg1	evidence					759:766	Accumulating evidence	746:766	Accumulating evidence	746:766	Accumulating evidence suggest that mesenchymal stem cells (MSCs) and anchor points consisting of tripeptides arg-gly-asp (RGD) have cytoprotective effects on pancreatic islets.					
32513541	1	85	theme	allogenic	363:371	arg1	transplantation					379:393	allogenic islet transplantation	363:393	allogenic islet transplantation	363:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	6	86	dep	MSCs	1325:1328	arg1	in vitro					1429:1436	in vitro	1429:1436	in vitro	1429:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	8	87	theme	reference	1640:1648	arg1	capsules					1666:1673	reference M-rich alginate capsules	1640:1673	reference M-rich alginate capsules	1640:1673	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	9	88	theme	RGD-G	1910:1914	arg1	alginate					1921:1928	the RGD-G rich alginate	1906:1928	the RGD-G rich alginate	1906:1928	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	2	89	from	system	534:539	arg1	islets					511:516	islets	511:516	islets from the immune system	511:539	In this context, microencapsulation offers the prospects of immunosuppressive-free therapy by physically isolating islets from the immune system.					
32513541	6	90	dep	METHODS	1131:1137	arg1	analyzed					1242:1249	analyzed	1242:1249	analyzed the empty capsules biocompatibility	1242:1285	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	90	dep	METHODS	1131:1137	arg1	investigated					1296:1307	investigated	1296:1307	investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro	1296:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	90	dep	METHODS	1131:1137	arg1	We					1139:1140	We	1139:1140	We	1139:1140	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	0	91	theme	human	9:13	arg1	islets					15:20	Improved human islets	0:20	Improved human islets' viability and functionality with mesenchymal stem cells	0:77	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	91	theme	human	9:13	arg1	functionality					37:49	functionality	37:49	functionality	37:49	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	0	91	theme	human	9:13	arg1	viability					23:31	viability	23:31	viability	23:31	Improved human islets' viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro.					
32513541	9	92	theme	alginate	1921:1928	arg1	combination					1877:1887	the combination	1873:1887	the combination of both MSCs and the RGD-G rich alginate	1873:1928	This side-effect of encapsulation was in part counteracted by the presence of MSCs but the restoration was complete with the combination of both MSCs and the RGD-G rich alginate.					
32513541	10	93	theme	composite	2000:2008	arg1	environment					2010:2020	a favorable composite environment	1988:2020	a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate	1988:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	6	94	from	islets	1422:1427	arg1	benefits					1313:1320	the benefits	1309:1320	the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro	1309:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	5	95	theme	RGD-G	1027:1031	arg1	alginate					1038:1045	RGD-G rich alginate	1027:1045	RGD-G rich alginate	1027:1045	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	10	96	theme	favorable	1990:1998	arg1	environment					2010:2020	a favorable composite environment	1988:2020	a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate	1988:2094	CONCLUSIONS The present findings show that bioprocessing a favorable composite environment inside the M-rich alginate capsule with both MSCs and RGD-G rich alginate improves human islets survival and functionality in vitro.					
32513541	1	97	dep	INTRODUCTION	171:182	arg1	compromised					272:282	compromised	272:282	is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation	269:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	2	98	theme	immunosuppressive-free	456:477	arg1	therapy					479:485	immunosuppressive-free therapy	456:485	immunosuppressive-free therapy	456:485	In this context, microencapsulation offers the prospects of immunosuppressive-free therapy by physically isolating islets from the immune system.					
32513541	5	99	theme	pancreatic	1084:1093	arg1	islets					1095:1100	human pancreatic islets viability and functionality	1078:1128	human pancreatic islets viability and functionality	1078:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	99	theme	pancreatic	1084:1093	arg1	functionality					1116:1128	functionality	1116:1128	functionality	1116:1128	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	5	99	theme	pancreatic	1084:1093	arg1	viability					1102:1110	viability	1102:1110	viability	1102:1110	Here, we investigated the effect of supplementing reference M-rich alginate microcapsules with MSCs and RGD-G rich alginate on bioprocessing as well as on human pancreatic islets viability and functionality.					
32513541	2	100	theme	immune	527:532	arg1	system					534:539	the immune system	523:539	the immune system	523:539	In this context, microencapsulation offers the prospects of immunosuppressive-free therapy by physically isolating islets from the immune system.					
32513541	1	101	theme	immunosuppressant	324:340	arg1	therapy					342:348	the immunosuppressant therapy	320:348	the immunosuppressant therapy required for allogenic islet transplantation	320:393	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	8	102	theme	alginate	1657:1664	arg1	capsules					1666:1673	reference M-rich alginate capsules	1640:1673	reference M-rich alginate capsules	1640:1673	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	6	103	theme	composite	1220:1228	arg1	product					1230:1236	the new microcapsule composite product	1199:1236	the new microcapsule composite product	1199:1236	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	3	104	theme	current	551:557	arg1	biomaterials					559:570	current biomaterials	551:570	current biomaterials	551:570	However, current biomaterials need to be optimized to: improve biocompatibility, guaranty the maintenance of graft viability and functionality, and prevent fibrosis overgrowth around the capsule in vivo.					
32513541	6	105	theme	MSCs	1325:1328	arg1	benefits					1313:1320	the benefits	1309:1320	the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro	1309:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	106	theme	new	1203:1205	arg1	product					1230:1236	the new microcapsule composite product	1199:1236	the new microcapsule composite product	1199:1236	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	6	107	from	viability	1357:1365	arg1	benefits					1313:1320	the benefits	1309:1320	the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro	1309:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32513541	1	108	theme	patients	260:267	arg1	number					234:239	a wider number	226:239	a wider number of type 1 diabetes patients	226:267	INTRODUCTION The extension of islet transplantation to a wider number of type 1 diabetes patients is compromised by severe adverse events related to the immunosuppressant therapy required for allogenic islet transplantation.					
32513541	8	109	from	Encapsulation	1623:1635	arg1	capsules					1666:1673	reference M-rich alginate capsules	1640:1673	reference M-rich alginate capsules	1640:1673	RESULTS Encapsulation in reference M-rich alginate capsules induced a reduction in viability and functionality compared to naked islets.					
32513541	6	110	from	functionality	1371:1383	arg1	benefits					1313:1320	the benefits	1309:1320	the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro	1309:1436	METHODS We characterized the microcapsules components, and then for the new microcapsule composite product: we analyzed the empty capsules biocompatibility and then investigated the benefits of MSCs and RGD-G rich alginate on viability and functionality on the encapsulated human pancreatic islets in vitro.					
32536397	5	0	theme	profiles	1126:1133	arg1	variation					1100:1108	the variation	1096:1108	the variation of drug release profiles among batches	1096:1147	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	3	1	theme	MeO	629:631	arg1	contents					641:648	the MeO and HPO contents	625:648	contents	641:648	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	1	2	theme	commercial	220:229	arg1	tablets					248:254	commercial extended-release tablets	220:254	commercial extended-release tablets	220:254	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	1	3	theme	extended-release	231:246	arg1	tablets					248:254	commercial extended-release tablets	220:254	commercial extended-release tablets	220:254	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	5	4	theme	MeO	1024:1026	arg1	content					1028:1034	the MeO content	1020:1034	the MeO content of HPMC	1020:1042	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	3	5	theme	HPO	637:639	arg1	contents					641:648	the MeO and HPO contents	625:648	contents	641:648	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	3	6	theme	HPMC	653:656	arg1	contents					641:648	the MeO and HPO contents	625:648	contents	641:648	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	2	7	from	sources	484:490	arg1	tablets					463:469	metformin hydrochloride extended-release tablets	422:469	metformin hydrochloride extended-release tablets from various sources	422:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	2	7	from	sources	484:490	arg1	batches					411:417	Twenty-seven batches	398:417	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources	398:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	4	8	theme	in	909:910	arg1	behavior					918:925	in vitro behavior	909:925	in vitro behavior	909:925	Additionally, the dissolution test was performed to evaluate the relationship between HPMC substitution pattern and in vitro behavior.					
32536397	1	9	theme	in	351:352	arg1	performance					360:370	the in vitro performance	347:370	the in vitro performance of these pharmaceuticals	347:395	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	0	10	theme	drug	93:96	arg1	release					98:104	the drug release	89:104	the drug release	89:104	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	0	11	theme	Raman	2:6	arg1	technique					22:30	A Raman imaging-based technique	0:30	A Raman imaging-based technique	0:30	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	5	12	theme	drug	1113:1116	arg1	profiles					1126:1133	drug release profiles	1113:1133	drug release profiles	1113:1133	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	4	13	theme	substitution	884:895	arg1	pattern					897:903	HPMC substitution pattern	879:903	HPMC substitution pattern	879:903	Additionally, the dissolution test was performed to evaluate the relationship between HPMC substitution pattern and in vitro behavior.					
32536397	3	14	theme	Raman	739:743	arg1	intensity					745:753	the Raman intensity	735:753	the Raman intensity of corresponding characteristic band	735:790	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	3	15	theme	proposed	698:705	arg1	method					719:724	a newly proposed calculation method	690:724	a newly proposed calculation method based on the Raman intensity of corresponding characteristic band	690:790	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	2	16	theme	tablets	463:469	arg1	batches					411:417	Twenty-seven batches	398:417	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources	398:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	3	17	theme	corresponding	758:770	arg1	band					787:790	corresponding characteristic band	758:790	corresponding characteristic band	758:790	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	4	18	theme	HPMC	879:882	arg1	pattern					897:903	HPMC substitution pattern	879:903	HPMC substitution pattern	879:903	Additionally, the dissolution test was performed to evaluate the relationship between HPMC substitution pattern and in vitro behavior.					
32536397	3	19	used	used	563:566	arg2	imaging					551:557	Raman imaging	545:557	Raman imaging	545:557	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	2	20	theme	extended-release	446:461	arg1	tablets					463:469	metformin hydrochloride extended-release tablets	422:469	metformin hydrochloride extended-release tablets from various sources	422:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	3	21	theme	characteristic	772:785	arg1	band					787:790	corresponding characteristic band	758:790	corresponding characteristic band	758:790	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	0	22	theme	commercial	109:118	arg1	tablets					137:143	commercial extended-release tablets	109:143	commercial extended-release tablets	109:143	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	5	23	theme	drug	967:970	arg1	rate					980:983	the drug release rate	963:983	the drug release rate	963:983	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	2	24	theme	metformin	422:430	arg1	tablets					463:469	metformin hydrochloride extended-release tablets	422:469	metformin hydrochloride extended-release tablets from various sources	422:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	0	25	theme	imaging-based	8:20	arg1	technique					22:30	A Raman imaging-based technique	0:30	A Raman imaging-based technique	0:30	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	1	26	theme	innovative	271:280	arg1	technique					311:319	an innovative Raman imaging-based analysis technique	268:319	an innovative Raman imaging-based analysis technique	268:319	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	0	27	from	effects	78:84	arg1	release					98:104	the drug release	89:104	the drug release	89:104	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	5	28	theme	release	972:978	arg1	rate					980:983	the drug release rate	963:983	the drug release rate	963:983	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	1	29	from	effects	336:342	arg1	performance					360:370	the in vitro performance	347:370	the in vitro performance of these pharmaceuticals	347:395	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	1	30	theme	Raman	282:286	arg1	technique					311:319	an innovative Raman imaging-based analysis technique	268:319	an innovative Raman imaging-based analysis technique	268:319	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	0	31	theme	tablets	137:143	arg1	contents					59:66	HPMC substituent contents	42:66	HPMC substituent contents	42:66	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	0	31	theme	tablets	137:143	arg1	effects					78:84	their effects	72:84	their effects on the drug release of commercial extended-release tablets	72:143	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	3	32	theme	tablets	616:622	arg1	composition					597:607	the composition	593:607	the composition of the tablets	593:622	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	4	33	dep	in	909:910	arg1	vitro					912:916	vitro	912:916	vitro	912:916	Additionally, the dissolution test was performed to evaluate the relationship between HPMC substitution pattern and in vitro behavior.					
32536397	1	34	theme	imaging-based	288:300	arg1	technique					311:319	an innovative Raman imaging-based analysis technique	268:319	an innovative Raman imaging-based analysis technique	268:319	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	0	35	theme	extended-release	120:135	arg1	tablets					137:143	commercial extended-release tablets	109:143	commercial extended-release tablets	109:143	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	1	36	theme	pharmaceuticals	381:395	arg1	performance					360:370	the in vitro performance	347:370	the in vitro performance of these pharmaceuticals	347:395	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	0	37	theme	substituent	47:57	arg1	contents					59:66	HPMC substituent contents	42:66	HPMC substituent contents	42:66	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	1	38	theme	analysis	302:309	arg1	technique					311:319	an innovative Raman imaging-based analysis technique	268:319	an innovative Raman imaging-based analysis technique	268:319	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	0	39	from	contents	59:66	arg1	release					98:104	the drug release	89:104	the drug release	89:104	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	5	40	theme	HPMC	1039:1042	arg1	content					1028:1034	the MeO content	1020:1034	the MeO content of HPMC	1020:1042	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	0	41	theme	HPMC	42:45	arg1	contents					59:66	HPMC substituent contents	42:66	HPMC substituent contents	42:66	A Raman imaging-based technique to assess HPMC substituent contents and their effects on the drug release of commercial extended-release tablets.					
32536397	3	42	theme	band	787:790	arg1	intensity					745:753	the Raman intensity	735:753	the Raman intensity of corresponding characteristic band	735:790	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	5	43	theme	release	1118:1124	arg1	profiles					1126:1133	drug release profiles	1113:1133	drug release profiles	1113:1133	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	2	44	theme	various	476:482	arg1	sources					484:490	various sources	476:490	various sources	476:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	1	45	dep	in	351:352	arg1	vitro					354:358	vitro	354:358	vitro	354:358	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	4	46	theme	dissolution	811:821	arg1	test					823:826	the dissolution test	807:826	the dissolution test	807:826	Additionally, the dissolution test was performed to evaluate the relationship between HPMC substitution pattern and in vitro behavior.					
32536397	5	47	theme	high	1055:1058	arg1	content					1064:1070	the high HPO content	1051:1070	the high HPO content	1051:1070	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	1	48	dep	contents	195:202	arg1	substitute					184:193	substitute	184:193	substitute	184:193	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	2	49	from	batches	411:417	arg1	sources					484:490	various sources	476:490	various sources	476:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	1	50	theme	HPMC	207:210	arg1	contents					195:202	the substitute contents	180:202	the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique	180:319	In this study, we tried to assess the substitute contents of HPMC used in commercial extended-release tablets directly by an innovative Raman imaging-based analysis technique and find their effects on the in vitro performance of these pharmaceuticals.					
32536397	2	51	theme	mainland	522:529	arg1	market					531:536	the Chinese mainland market	510:536	the Chinese mainland market	510:536	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	5	52	theme	HPO	1060:1062	arg1	content					1064:1070	the high HPO content	1051:1070	the high HPO content	1051:1070	In sum, our findings indicate that the drug release rate can be downregulated by increasing the MeO content of HPMC, while the high HPO content would largely eliminate the variation of drug release profiles among batches.					
32536397	2	53	theme	Chinese	514:520	arg1	market					531:536	the Chinese mainland market	510:536	the Chinese mainland market	510:536	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	3	54	theme	calculation	707:717	arg1	method					719:724	a newly proposed calculation method	690:724	a newly proposed calculation method based on the Raman intensity of corresponding characteristic band	690:790	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32536397	2	55	theme	hydrochloride	432:444	arg1	tablets					463:469	metformin hydrochloride extended-release tablets	422:469	metformin hydrochloride extended-release tablets from various sources	422:490	Twenty-seven batches of metformin hydrochloride extended-release tablets from various sources were collected in the Chinese mainland market.					
32536397	3	56	theme	Raman	545:549	arg1	imaging					551:557	Raman imaging	545:557	Raman imaging	545:557	While Raman imaging was used to qualitatively analyze the composition of the tablets, the MeO and HPO contents of HPMC were quantitatively assessed by a newly proposed calculation method based on the Raman intensity of corresponding characteristic band.					
32328933	0	0	theme	Therapy	94:100	arg1	Course					102:107	Experimental Therapy Course	81:107	Experimental Therapy Course	81:107	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	4	1	theme	a	840:840	arg1	week					842:845	2 times a week	832:845	2 times a week	832:845	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	4	1	theme	a	840:840	arg1	inhalations					819:829	12 inhalations	816:829	12 inhalations	816:829	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	1	2	theme	individual	165:174	arg1	efficacy					153:160	antituberculous efficacy	137:160	antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose	137:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	0	3	theme	Experimental	81:92	arg1	Course					102:107	Experimental Therapy Course	81:107	Experimental Therapy Course	81:107	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	4	4	theme	times	834:838	arg1	week					842:845	2 times a week	832:845	2 times a week	832:845	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	4	4	theme	times	834:838	arg1	inhalations					819:829	12 inhalations	816:829	12 inhalations	816:829	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	1	5	from	individual	165:174	arg1	standard					327:334	standard	327:334	standard	327:334	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	5	from	individual	165:174	arg1	dose					364:367	standard (15 mg/kg) or low (3 mg/kg) dose	327:367	dose	364:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	5	from	individual	165:174	arg1	mg/kg					340:344	15 mg/kg	337:344	15 mg/kg	337:344	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	5	from	individual	165:174	arg1	mg/kg					357:361	3 mg/kg	355:361	3 mg/kg	355:361	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	3	6	theme	liposomal	512:520	arg1	form					522:525	liposomal form	512:525	liposomal form of dextrazide and isoniazid	512:553	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	1	7	from	dose	364:367	arg1	individual					165:174	individual	165:174	individual	165:174	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	7	from	dose	364:367	arg1	efficacy					153:160	antituberculous efficacy	137:160	antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose	137:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	8	dep	inhaled	316:322	arg1	composition					259:269	a composition	257:269	a composition consisted of isoniazid and oxidized dextran	257:313	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	8	dep	inhaled	316:322	arg1	form					237:240	liposomal form	227:240	liposomal form of dextrazide	227:254	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	8	dep	inhaled	316:322	arg1	administration					188:201	combined administration	179:201	combined administration of "free" isoniazid	179:221	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	9	from	efficacy	153:160	arg1	standard					327:334	standard	327:334	standard	327:334	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	9	from	efficacy	153:160	arg1	dose					364:367	standard (15 mg/kg) or low (3 mg/kg) dose	327:367	dose	364:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	9	from	efficacy	153:160	arg1	mg/kg					340:344	15 mg/kg	337:344	15 mg/kg	337:344	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	9	from	efficacy	153:160	arg1	mg/kg					357:361	3 mg/kg	355:361	3 mg/kg	355:361	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	3	10	theme	form	522:525	arg1	inhalation					498:507	Combined inhalation	489:507	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose	489:569	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	4	11	theme	minor	703:707	arg1	effect					724:729	A minor prodestructive effect	701:729	A minor prodestructive effect of this combination	701:749	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	2	12	dep	month	392:396	arg1	contamination					404:416	contamination	404:416	contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv	404:486	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	4	13	dep	therapy	807:813	arg1	week					842:845	2 times a week	832:845	2 times a week	832:845	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	4	13	dep	therapy	807:813	arg1	inhalations					819:829	12 inhalations	816:829	12 inhalations	816:829	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	1	14	theme	combined	179:186	arg1	administration					188:201	combined administration	179:201	combined administration of "free" isoniazid	179:221	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	2	15	theme	strain	475:480	arg1	H37Rv					482:486	Mycobacterium tuberculosis strain H37Rv	448:486	Mycobacterium tuberculosis strain H37Rv	448:486	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	1	16	theme	low	350:352	arg1	dose					364:367	standard (15 mg/kg) or low (3 mg/kg) dose	327:367	dose	364:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	16	theme	low	350:352	arg1	mg/kg					357:361	3 mg/kg	355:361	3 mg/kg	355:361	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	2	17	theme	male	433:436	arg1	mice					438:441	outbred ICR male mice	421:441	outbred ICR male mice	421:441	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	2	18	theme	mice	438:441	arg1	contamination					404:416	contamination	404:416	contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv	404:486	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	3	19	theme	isoniazid	545:553	arg1	form					522:525	liposomal form	512:525	liposomal form of dextrazide and isoniazid	512:553	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	4	20	theme	combination	739:749	arg1	effect					724:729	A minor prodestructive effect	701:729	A minor prodestructive effect of this combination	701:749	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	1	21	theme	free	207:210	arg1	isoniazid					213:221	"free" isoniazid	206:221	"free" isoniazid	206:221	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	0	22	theme	Inhalations	12:22	arg1	Efficacy					0:7	Efficacy	0:7	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.	0:116	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	3	23	theme	due	625:627	arg1	tuberculosis					612:623	mycobacterium tuberculosis	598:623	mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity	598:698	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	3	24	theme	dextrazide	530:539	arg1	form					522:525	liposomal form	512:525	liposomal form of dextrazide and isoniazid	512:553	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	2	25	theme	outbred	421:427	arg1	mice					438:441	outbred ICR male mice	421:441	outbred ICR male mice	421:441	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	1	26	theme	"	211:211	arg1	isoniazid					213:221	"free" isoniazid	206:221	"free" isoniazid	206:221	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	0	27	theme	Compositions	43:54	arg1	Inhalations					12:22	Inhalations	12:22	Inhalations of Antituberculous Compositions	12:54	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	1	28	theme	oxidized	298:305	arg1	dextran					307:313	oxidized dextran	298:313	oxidized dextran	298:313	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	3	29	theme	low	562:564	arg1	dose					566:569	the low dose	558:569	the low dose	558:569	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	1	30	theme	isoniazid	213:221	arg1	administration					188:201	combined administration	179:201	combined administration of "free" isoniazid	179:221	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	30	theme	isoniazid	213:221	arg1	form					237:240	liposomal form	227:240	liposomal form of dextrazide	227:254	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	0	31	theme	Antituberculous	27:41	arg1	Compositions					43:54	Antituberculous Compositions	27:54	Antituberculous Compositions	27:54	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	2	32	theme	ICR	429:431	arg1	mice					438:441	outbred ICR male mice	421:441	outbred ICR male mice	421:441	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	3	33	theme	mycobacterium	598:610	arg1	tuberculosis					612:623	mycobacterium tuberculosis	598:623	mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity	598:698	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	0	34	theme	Different	61:69	arg1	Length					71:76	Different Length	61:76	Different Length of Experimental Therapy Course in Mice	61:115	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	0	35	from	Length	71:76	arg1	Mice					112:115	Mice	112:115	Mice	112:115	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	1	36	theme	inhaled	316:322	arg1	efficacy					153:160	antituberculous efficacy	137:160	antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose	137:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	3	37	theme	pulmonary	658:666	arg1	effect					668:673	diminished prodestructive pulmonary effect	632:673	diminished prodestructive pulmonary effect	632:673	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	4	38	theme	therapy	807:813	arg1	onset					798:802	the onset	794:802	the onset of therapy (12 inhalations, 2 times a week)	794:846	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	2	39	with	contamination	404:416	arg1	H37Rv					482:486	Mycobacterium tuberculosis strain H37Rv	448:486	Mycobacterium tuberculosis strain H37Rv	448:486	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	0	40	with	Efficacy	0:7	arg1	Length					71:76	Different Length	61:76	Different Length of Experimental Therapy Course in Mice	61:115	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
32328933	4	41	theme	prodestructive	709:722	arg1	effect					724:729	A minor prodestructive effect	701:729	A minor prodestructive effect of this combination	701:749	A minor prodestructive effect of this combination was observed starting from 1.5 month after the onset of therapy (12 inhalations, 2 times a week), and it augmented after 24 inhalations administered during 3 months.					
32328933	1	42	theme	liposomal	227:235	arg1	form					237:240	liposomal form	227:240	liposomal form of dextrazide	227:254	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	3	43	theme	diminished	632:641	arg1	effect					668:673	diminished prodestructive pulmonary effect	632:673	diminished prodestructive pulmonary effect	632:673	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	1	44	theme	antituberculous	137:151	arg1	efficacy					153:160	antituberculous efficacy	137:160	antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose	137:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	2	45	theme	tuberculosis	462:473	arg1	H37Rv					482:486	Mycobacterium tuberculosis strain H37Rv	448:486	Mycobacterium tuberculosis strain H37Rv	448:486	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	3	46	theme	prodestructive	643:656	arg1	effect					668:673	diminished prodestructive pulmonary effect	632:673	diminished prodestructive pulmonary effect	632:673	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	3	47	theme	Combined	489:496	arg1	inhalation					498:507	Combined inhalation	489:507	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose	489:569	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	1	48	from	standard	327:334	arg1	individual					165:174	individual	165:174	individual	165:174	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	48	from	standard	327:334	arg1	efficacy					153:160	antituberculous efficacy	137:160	antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose	137:367	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	3	49	theme	low	681:683	arg1	hepatotoxicity					685:698	a low hepatotoxicity	679:698	a low hepatotoxicity	679:698	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	1	50	theme	dextrazide	245:254	arg1	administration					188:201	combined administration	179:201	combined administration of "free" isoniazid	179:221	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	1	50	theme	dextrazide	245:254	arg1	form					237:240	liposomal form	227:240	liposomal form of dextrazide	227:254	The study compared antituberculous efficacy of individual or combined administration of "free" isoniazid and liposomal form of dextrazide (a composition consisted of isoniazid and oxidized dextran) inhaled in standard (15 mg/kg) or low (3 mg/kg) dose.					
32328933	2	51	theme	Mycobacterium	448:460	arg1	H37Rv					482:486	Mycobacterium tuberculosis strain H37Rv	448:486	Mycobacterium tuberculosis strain H37Rv	448:486	The therapy started 1 month after contamination of outbred ICR male mice with Mycobacterium tuberculosis strain H37Rv.					
32328933	3	52	from	inhalation	498:507	arg1	dose					566:569	the low dose	558:569	the low dose	558:569	Combined inhalation of liposomal form of dextrazide and isoniazid in the low dose was most effective against mycobacterium tuberculosis due to diminished prodestructive pulmonary effect and a low hepatotoxicity.					
32328933	0	53	theme	Course	102:107	arg1	Length					71:76	Different Length	61:76	Different Length of Experimental Therapy Course in Mice	61:115	Efficacy of Inhalations of Antituberculous Compositions with Different Length of Experimental Therapy Course in Mice.					
34921749	4	0	theme	intestinal	505:514	arg1	transcriptomes					527:540	intestinal epithelial transcriptomes	505:540	intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs)	505:571	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	9	1	theme	divergent	1326:1334	arg1	mechanisms					1336:1345	divergent mechanisms	1326:1345	divergent mechanisms	1326:1345	Interestingly, most sHMOs protect pups against NEC, exerting divergent mechanisms on intestinal cell morphology and inflammation.					
34921749	1	2	theme	SCOPE	172:176	arg1	NEC					205:207	NEC	205:207	NEC	205:207	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	1	2	theme	SCOPE	172:176	arg1	enterocolitis					190:202	SCOPE Necrotizing enterocolitis	172:202	SCOPE Necrotizing enterocolitis (NEC)	172:208	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	1	2	theme	SCOPE	172:176	arg1	emergency					244:252	a devastating gastrointestinal emergency	213:252	a devastating gastrointestinal emergency affecting preterm infants	213:278	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	10	3	dep	CONCLUSIONS	1395:1405	arg1	demonstrate					1421:1431	demonstrate	1421:1431	demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.	1421:1534	CONCLUSIONS These results demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.					
34921749	6	4	theme	HMO-dependent	1011:1023	arg1	manner					1025:1030	an HMO-dependent manner	1008:1030	an HMO-dependent manner	1008:1030	Protection against intestinal barrier dysfunction and inflammation occurred in an HMO-dependent manner.					
34921749	7	5	theme	changes	1085:1091	arg1	set					1059:1061	a unique set	1050:1061	a unique set of host transcriptome changes and modulated unique signaling pathways	1050:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	5	theme	changes	1085:1091	arg1	pathways					1124:1131	modulated unique signaling pathways	1097:1131	modulated unique signaling pathways	1097:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	5	theme	changes	1085:1091	arg1	changes					1085:1091	host transcriptome changes	1066:1091	host transcriptome changes	1066:1091	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	0	6	from	Relationships	19:31	arg1	Transcriptome					93:105	the Intestinal Epithelial Transcriptome	67:105	the Intestinal Epithelial Transcriptome in Caco-2 Cells	67:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	0	6	from	Relationships	19:31	arg1	Model					136:140	a Murine Model	127:140	a Murine Model of Necrotizing Enterocolitis	127:169	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	10	7	theme	intestinal	1478:1487	arg1	physiology					1489:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	CONCLUSIONS These results demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.					
34921749	1	8	theme	Necrotizing	178:188	arg1	NEC					205:207	NEC	205:207	NEC	205:207	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	1	8	theme	Necrotizing	178:188	arg1	enterocolitis					190:202	SCOPE Necrotizing enterocolitis	172:202	SCOPE Necrotizing enterocolitis (NEC)	172:208	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	1	8	theme	Necrotizing	178:188	arg1	emergency					244:252	a devastating gastrointestinal emergency	213:252	a devastating gastrointestinal emergency affecting preterm infants	213:278	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	4	9	theme	structure-function	586:603	arg1	relationships					605:617	structure-function relationships	586:617	structure-function relationships of HMOs on intestinal signaling	586:649	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	4	10	from	HMOs	622:625	arg1	signaling					641:649	intestinal signaling	630:649	intestinal signaling	630:649	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	7	11	theme	pathways	1124:1131	arg1	set					1059:1061	a unique set	1050:1061	a unique set of host transcriptome changes and modulated unique signaling pathways	1050:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	11	theme	pathways	1124:1131	arg1	pathways					1124:1131	modulated unique signaling pathways	1097:1131	modulated unique signaling pathways	1097:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	11	theme	pathways	1124:1131	arg1	changes					1085:1091	host transcriptome changes	1066:1091	host transcriptome changes	1066:1091	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	11	12	theme	HMOs	1606:1609	arg1	relationship					1590:1601	the first structure-function relationship	1561:1601	the first structure-function relationship of HMOs in the context of intestinal cell signaling responses	1561:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	4	13	theme	HMOs	622:625	arg1	relationships					605:617	structure-function relationships	586:617	structure-function relationships of HMOs on intestinal signaling	586:649	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	0	14	theme	Caco-2	110:115	arg1	Cells					117:121	Caco-2 Cells	110:121	Caco-2 Cells	110:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	10	15	theme	HMOs	1466:1469	arg1	physiology					1489:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	CONCLUSIONS These results demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.					
34921749	11	16	theme	structure-function	1571:1588	arg1	relationship					1590:1601	the first structure-function relationship	1561:1601	the first structure-function relationship of HMOs in the context of intestinal cell signaling responses	1561:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	3	17	theme	HMO	364:366	arg1	compositions					368:379	HMO compositions	364:379	HMO compositions	364:379	HMO compositions are highly diverse, and it is unclear if anti-NEC properties are specific to carbohydrate motifs.					
34921749	11	18	theme	HMO-like	1729:1736	arg1	compounds					1738:1746	HMO-like compounds	1729:1746	HMO-like compounds	1729:1746	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	7	19	theme	unique	1107:1112	arg1	pathways					1124:1131	modulated unique signaling pathways	1097:1131	modulated unique signaling pathways	1097:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	11	20	theme	first	1565:1569	arg1	relationship					1590:1601	the first structure-function relationship	1561:1601	the first structure-function relationship of HMOs in the context of intestinal cell signaling responses	1561:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	11	21	theme	responses	1655:1663	arg1	context					1618:1624	the context	1614:1624	the context of intestinal cell signaling responses	1614:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	5	22	dep	RESULTS	664:670	arg1	interrogates					683:694	interrogates	683:694	interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing	683:796	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	10	23	theme	action	1521:1526	arg1	mechanisms					1507:1516	their mechanisms	1501:1516	their mechanisms of action	1501:1526	CONCLUSIONS These results demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.					
34921749	4	24	from	relationships	605:617	arg1	signaling					641:649	intestinal signaling	630:649	intestinal signaling	630:649	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	9	25	theme	cell	1361:1364	arg1	morphology					1366:1375	intestinal cell morphology	1350:1375	intestinal cell morphology	1350:1375	Interestingly, most sHMOs protect pups against NEC, exerting divergent mechanisms on intestinal cell morphology and inflammation.					
34921749	5	26	theme	Caco-2Bbe1	717:726	arg1	cells					728:732	Caco-2Bbe1 cells	717:732	Caco-2Bbe1 cells	717:732	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	0	27	theme	Structure-Function	0:17	arg1	Relationships					19:31	Structure-Function Relationships	0:31	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.	0:170	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	7	28	theme	modulated	1097:1105	arg1	pathways					1124:1131	modulated unique signaling pathways	1097:1131	modulated unique signaling pathways	1097:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	29	theme	host	1066:1069	arg1	changes					1085:1091	host transcriptome changes	1066:1091	host transcriptome changes	1066:1091	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	2	30	theme	milk	334:337	arg1	HMOs					357:360	HMOs	357:360	HMOs	357:360	Breastmilk protects against NEC, partly due to human milk oligosaccharides (HMOs).					
34921749	2	30	theme	milk	334:337	arg1	oligosaccharides					339:354	human milk oligosaccharides	328:354	human milk oligosaccharides (HMOs)	328:361	Breastmilk protects against NEC, partly due to human milk oligosaccharides (HMOs).					
34921749	0	31	theme	Human	36:40	arg1	Oligosaccharides					47:62	Human Milk Oligosaccharides	36:62	Human Milk Oligosaccharides	36:62	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	7	32	theme	transcriptome	1071:1083	arg1	changes					1085:1091	host transcriptome changes	1066:1091	host transcriptome changes	1066:1091	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	2	33	theme	human	328:332	arg1	HMOs					357:360	HMOs	357:360	HMOs	357:360	Breastmilk protects against NEC, partly due to human milk oligosaccharides (HMOs).					
34921749	2	33	theme	human	328:332	arg1	oligosaccharides					339:354	human milk oligosaccharides	328:354	human milk oligosaccharides (HMOs)	328:361	Breastmilk protects against NEC, partly due to human milk oligosaccharides (HMOs).					
34921749	5	34	dep	interrogates	683:694	arg1	2'-FL					818:822	2'-FL	818:822	2'-FL	818:822	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	5	34	dep	interrogates	683:694	arg1	6'-siallyllactose					850:866	6'-siallyllactose	850:866	6'-siallyllactose (6'-SL)	850:874	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	5	34	dep	interrogates	683:694	arg1	3-fucosyllactose					826:841	3-fucosyllactose	826:841	3-fucosyllactose (3FL)	826:847	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	5	34	dep	interrogates	683:694	arg1	2'-fucosyllactose					799:815	2'-fucosyllactose	799:815	2'-fucosyllactose (2'-FL)	799:823	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	5	34	dep	interrogates	683:694	arg1	lacto-N-neotetraose					901:919	lacto-N-neotetraose	901:919	lacto-N-neotetraose (LNnT)	901:926	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	5	34	dep	interrogates	683:694	arg1	lacto-N-tetraose					877:892	lacto-N-tetraose	877:892	lacto-N-tetraose (LNT)	877:898	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	9	35	theme	intestinal	1350:1359	arg1	morphology					1366:1375	intestinal cell morphology	1350:1375	intestinal cell morphology	1350:1375	Interestingly, most sHMOs protect pups against NEC, exerting divergent mechanisms on intestinal cell morphology and inflammation.					
34921749	0	36	theme	Murine	129:134	arg1	Model					136:140	a Murine Model	127:140	a Murine Model of Necrotizing Enterocolitis	127:169	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	5	37	dep	lacto-N-tetraose	877:892	arg1	LNT					895:897	LNT	895:897	LNT	895:897	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	11	38	from	HMOs	1606:1609	arg1	context					1618:1624	the context	1614:1624	the context of intestinal cell signaling responses	1614:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	8	39	theme	side	1183:1186	arg1	chains					1188:1193	similar side chains	1175:1193	similar side chains	1175:1193	There is clustering between HMOs bearing similar side chains, with little overlap in gene regulation which is shared by all sHMOs.					
34921749	0	40	theme	Oligosaccharides	47:62	arg1	Relationships					19:31	Structure-Function Relationships	0:31	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.	0:170	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	6	41	theme	barrier	959:965	arg1	dysfunction					967:977	intestinal barrier dysfunction	948:977	intestinal barrier dysfunction	948:977	Protection against intestinal barrier dysfunction and inflammation occurred in an HMO-dependent manner.					
34921749	0	42	theme	Necrotizing	145:155	arg1	Enterocolitis					157:169	Necrotizing Enterocolitis	145:169	Necrotizing Enterocolitis	145:169	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	0	43	from	Transcriptome	93:105	arg1	Cells					117:121	Caco-2 Cells	110:121	Caco-2 Cells	110:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	1	44	theme	devastating	215:225	arg1	emergency					244:252	a devastating gastrointestinal emergency	213:252	a devastating gastrointestinal emergency affecting preterm infants	213:278	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	1	44	theme	devastating	215:225	arg1	enterocolitis					190:202	SCOPE Necrotizing enterocolitis	172:202	SCOPE Necrotizing enterocolitis (NEC)	172:208	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	0	45	theme	Milk	42:45	arg1	Oligosaccharides					47:62	Human Milk Oligosaccharides	36:62	Human Milk Oligosaccharides	36:62	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	4	46	theme	synthetic	550:558	arg1	HMOs					560:563	five synthetic HMOs	545:563	five synthetic HMOs (sHMOs)	545:571	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	4	46	theme	synthetic	550:558	arg1	sHMOs					566:570	sHMOs	566:570	sHMOs	566:570	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	3	47	theme	anti-NEC	422:429	arg1	properties					431:440	anti-NEC properties	422:440	anti-NEC properties	422:440	HMO compositions are highly diverse, and it is unclear if anti-NEC properties are specific to carbohydrate motifs.					
34921749	1	48	theme	preterm	264:270	arg1	infants					272:278	preterm infants	264:278	preterm infants	264:278	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	11	49	theme	signaling	1645:1653	arg1	responses					1655:1663	intestinal cell signaling responses	1629:1663	intestinal cell signaling responses	1629:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	1	50	theme	gastrointestinal	227:242	arg1	emergency					244:252	a devastating gastrointestinal emergency	213:252	a devastating gastrointestinal emergency affecting preterm infants	213:278	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	1	50	theme	gastrointestinal	227:242	arg1	enterocolitis					190:202	SCOPE Necrotizing enterocolitis	172:202	SCOPE Necrotizing enterocolitis (NEC)	172:208	SCOPE Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency affecting preterm infants.					
34921749	5	51	from	transcriptome	700:712	arg1	response					737:744	response	737:744	response to five synthetic HMOs (sHMOs) using RNA sequencing	737:796	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	7	52	theme	unique	1052:1057	arg1	set					1059:1061	a unique set	1050:1061	a unique set of host transcriptome changes and modulated unique signaling pathways	1050:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	52	theme	unique	1052:1057	arg1	pathways					1124:1131	modulated unique signaling pathways	1097:1131	modulated unique signaling pathways	1097:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	7	52	theme	unique	1052:1057	arg1	changes					1085:1091	host transcriptome changes	1066:1091	host transcriptome changes	1066:1091	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	4	53	from	signaling	641:649	arg1	relationships					605:617	structure-function relationships	586:617	structure-function relationships of HMOs on intestinal signaling	586:649	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	8	54	dep	little	1201:1206	arg1	overlap					1208:1214	overlap	1208:1214	overlap in gene regulation which is shared by all sHMOs	1208:1262	There is clustering between HMOs bearing similar side chains, with little overlap in gene regulation which is shared by all sHMOs.					
34921749	6	55	theme	intestinal	948:957	arg1	dysfunction					967:977	intestinal barrier dysfunction	948:977	intestinal barrier dysfunction	948:977	Protection against intestinal barrier dysfunction and inflammation occurred in an HMO-dependent manner.					
34921749	5	56	theme	synthetic	754:762	arg1	sHMOs					770:774	sHMOs	770:774	sHMOs	770:774	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	5	56	theme	synthetic	754:762	arg1	HMOs					764:767	five synthetic HMOs	749:767	five synthetic HMOs (sHMOs) using RNA sequencing	749:796	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	0	57	theme	Enterocolitis	157:169	arg1	Transcriptome					93:105	the Intestinal Epithelial Transcriptome	67:105	the Intestinal Epithelial Transcriptome in Caco-2 Cells	67:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	0	57	theme	Enterocolitis	157:169	arg1	Model					136:140	a Murine Model	127:140	a Murine Model of Necrotizing Enterocolitis	127:169	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	7	58	theme	signaling	1114:1122	arg1	pathways					1124:1131	modulated unique signaling pathways	1097:1131	modulated unique signaling pathways	1097:1131	Each sHMO exerts a unique set of host transcriptome changes and modulated unique signaling pathways.					
34921749	11	59	theme	intestinal	1629:1638	arg1	responses					1655:1663	intestinal cell signaling responses	1629:1663	intestinal cell signaling responses	1629:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	10	60	theme	impact	1471:1476	arg1	physiology					1489:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	CONCLUSIONS These results demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.					
34921749	4	61	theme	HMOs	560:563	arg1	transcriptomes					527:540	intestinal epithelial transcriptomes	505:540	intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs)	505:571	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	5	62	theme	cells	728:732	arg1	transcriptome					700:712	the transcriptome	696:712	the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing	696:796	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	10	63	theme	distinct	1457:1464	arg1	physiology					1489:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	structurally distinct HMOs impact intestinal physiology	1444:1498	CONCLUSIONS These results demonstrate that while structurally distinct HMOs impact intestinal physiology, their mechanisms of action differ.					
34921749	5	64	theme	RNA	783:785	arg1	sequencing					787:796	RNA sequencing	783:796	RNA sequencing	783:796	METHODS AND RESULTS This study interrogates the transcriptome of Caco-2Bbe1 cells in response to five synthetic HMOs (sHMOs) using RNA sequencing: 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3FL), 6'-siallyllactose (6'-SL), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT).					
34921749	11	65	from	context	1618:1624	arg1	relationship					1590:1601	the first structure-function relationship	1561:1601	the first structure-function relationship of HMOs in the context of intestinal cell signaling responses	1561:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	4	66	theme	intestinal	630:639	arg1	signaling					641:649	intestinal signaling	630:649	intestinal signaling	630:649	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	11	67	theme	cell	1640:1643	arg1	responses					1655:1663	intestinal cell signaling responses	1629:1663	intestinal cell signaling responses	1629:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	8	68	theme	gene	1219:1222	arg1	regulation					1224:1233	gene regulation	1219:1233	gene regulation which is shared by all sHMOs	1219:1262	There is clustering between HMOs bearing similar side chains, with little overlap in gene regulation which is shared by all sHMOs.					
34921749	0	69	theme	Epithelial	82:91	arg1	Transcriptome					93:105	the Intestinal Epithelial Transcriptome	67:105	the Intestinal Epithelial Transcriptome in Caco-2 Cells	67:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	9	70	theme	most	1280:1283	arg1	sHMOs					1285:1289	most sHMOs	1280:1289	most sHMOs	1280:1289	Interestingly, most sHMOs protect pups against NEC, exerting divergent mechanisms on intestinal cell morphology and inflammation.					
34921749	3	71	theme	carbohydrate	458:469	arg1	motifs					471:476	carbohydrate motifs	458:476	carbohydrate motifs	458:476	HMO compositions are highly diverse, and it is unclear if anti-NEC properties are specific to carbohydrate motifs.					
34921749	11	72	theme	functional	1678:1687	arg1	framework					1689:1697	a functional framework	1676:1697	a functional framework by which to screen and design HMO-like compounds	1676:1746	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	0	73	from	Model	136:140	arg1	Cells					117:121	Caco-2 Cells	110:121	Caco-2 Cells	110:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	0	74	theme	Intestinal	71:80	arg1	Transcriptome					93:105	the Intestinal Epithelial Transcriptome	67:105	the Intestinal Epithelial Transcriptome in Caco-2 Cells	67:121	Structure-Function Relationships of Human Milk Oligosaccharides on the Intestinal Epithelial Transcriptome in Caco-2 Cells and a Murine Model of Necrotizing Enterocolitis.					
34921749	4	75	theme	epithelial	516:525	arg1	transcriptomes					527:540	intestinal epithelial transcriptomes	505:540	intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs)	505:571	Here, this study compares intestinal epithelial transcriptomes of five synthetic HMOs (sHMOs) and examines structure-function relationships of HMOs on intestinal signaling.					
34921749	11	76	from	relationship	1590:1601	arg1	context					1618:1624	the context	1614:1624	the context of intestinal cell signaling responses	1614:1663	This finding establishes the first structure-function relationship of HMOs in the context of intestinal cell signaling responses and offers a functional framework by which to screen and design HMO-like compounds.					
34921749	8	77	theme	similar	1175:1181	arg1	chains					1188:1193	similar side chains	1175:1193	similar side chains	1175:1193	There is clustering between HMOs bearing similar side chains, with little overlap in gene regulation which is shared by all sHMOs.					
32297820	0	0	theme	maxillofacial	82:94	arg1	engineering					108:118	maxillofacial bone tissue engineering	82:118	maxillofacial bone tissue engineering	82:118	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	0	1	from	effect	19:24	arg1	chitosan					60:67	composite chitosan	50:67	composite chitosan	50:67	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	3	2	theme	suitable	328:335	arg1	performance					352:362	suitable bone-mimicking performance	328:362	suitable bone-mimicking performance for maxillofacial bone tissue engineering	328:404	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	0	3	theme	tissue	101:106	arg1	engineering					108:118	maxillofacial bone tissue engineering	82:118	maxillofacial bone tissue engineering	82:118	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	2	4	from	effect	200:205	arg1	scaffolds					250:258	chitosan composite scaffolds	231:258	chitosan composite scaffolds	231:258	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds was evaluated.					
32297820	1	5	from	trend	150:154	arg1	biomaterials					159:170	biomaterials science	159:178	biomaterials science	159:178	This research explored a new trend in biomaterials science.					
32297820	3	6	theme	maxillofacial	368:380	arg1	engineering					394:404	maxillofacial bone tissue engineering	368:404	maxillofacial bone tissue engineering	368:404	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	0	7	theme	bone-mimicking	4:17	arg1	effect					19:24	The bone-mimicking effect	0:24	The bone-mimicking effect of calcium phosphate on composite chitosan	0:67	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	0	8	theme	bone	96:99	arg1	engineering					108:118	maxillofacial bone tissue engineering	82:118	maxillofacial bone tissue engineering	82:118	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	1	9	dep	biomaterials	159:170	arg1	science					172:178	science	172:178	science	172:178	This research explored a new trend in biomaterials science.					
32297820	3	10	theme	%	290:290	arg1	phosphate					300:308	2% calcium phosphate	289:308	2% calcium phosphate	289:308	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	3	11	theme	calcium	292:298	arg1	phosphate					300:308	2% calcium phosphate	289:308	2% calcium phosphate	289:308	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	2	12	theme	bone-mimicking	185:198	arg1	effect					200:205	The bone-mimicking effect	181:205	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds	181:258	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds was evaluated.					
32297820	0	13	theme	phosphate	37:45	arg1	effect					19:24	The bone-mimicking effect	0:24	The bone-mimicking effect of calcium phosphate on composite chitosan	0:67	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	0	14	theme	calcium	29:35	arg1	phosphate					37:45	calcium phosphate	29:45	calcium phosphate	29:45	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	3	15	theme	2	289:289	arg1	%					290:290	%	290:290	%	290:290	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	2	16	theme	composite	240:248	arg1	scaffolds					250:258	chitosan composite scaffolds	231:258	chitosan composite scaffolds	231:258	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds was evaluated.					
32297820	0	17	theme	composite	50:58	arg1	chitosan					60:67	composite chitosan	50:67	composite chitosan	50:67	The bone-mimicking effect of calcium phosphate on composite chitosan scaffolds in maxillofacial bone tissue engineering.					
32297820	3	18	theme	bone	382:385	arg1	engineering					394:404	maxillofacial bone tissue engineering	368:404	maxillofacial bone tissue engineering	368:404	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	2	19	theme	chitosan	231:238	arg1	scaffolds					250:258	chitosan composite scaffolds	231:258	chitosan composite scaffolds	231:258	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds was evaluated.					
32297820	2	20	theme	phosphate	218:226	arg1	effect					200:205	The bone-mimicking effect	181:205	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds	181:258	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds was evaluated.					
32297820	2	21	theme	calcium	210:216	arg1	phosphate					218:226	calcium phosphate	210:226	calcium phosphate	210:226	The bone-mimicking effect of calcium phosphate on chitosan composite scaffolds was evaluated.					
32297820	3	22	with	Chitosan	275:282	arg1	phosphate					300:308	2% calcium phosphate	289:308	2% calcium phosphate	289:308	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	3	23	theme	bone-mimicking	337:350	arg1	performance					352:362	suitable bone-mimicking performance	328:362	suitable bone-mimicking performance for maxillofacial bone tissue engineering	328:404	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	3	24	contain	have	323:326	arg2	performance					352:362	suitable bone-mimicking performance	328:362	suitable bone-mimicking performance for maxillofacial bone tissue engineering	328:404	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	3	24	contain	have	323:326	arg1	Chitosan					275:282	Chitosan	275:282	Chitosan with 2% calcium phosphate	275:308	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
32297820	1	25	theme	new	146:148	arg1	trend					150:154	a new trend	144:154	a new trend in biomaterials science	144:178	This research explored a new trend in biomaterials science.					
32297820	3	26	theme	tissue	387:392	arg1	engineering					394:404	maxillofacial bone tissue engineering	368:404	maxillofacial bone tissue engineering	368:404	Chitosan with 2% calcium phosphate was found to have suitable bone-mimicking performance for maxillofacial bone tissue engineering.					
34321166	2	0	theme	inhibitors	650:659	arg1	use					606:608	frequent use	597:608	frequent use of radial-artery access and novel P2Y12 inhibitors	597:659	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	2	0	theme	inhibitors	650:659	arg1	use					670:672	low use	666:672	low use of glycoprotein IIb/IIIa inhibitors (GPIs)	666:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	15	1	from	practice	2877:2884	arg1	risk					2844:2847	high risk	2839:2847	high risk of bleeding in contemporary practice	2839:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	15	1	from	practice	2877:2884	arg1	bleeding					2852:2859	bleeding	2852:2859	bleeding	2852:2859	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	10	2	used	used	2045:2048	arg2	approach					2032:2039	radial approach	2025:2039	radial approach	2025:2039	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	10	2	used	used	2045:2048	arg2	inhibitors					2009:2018	novel P2Y12 inhibitors	1997:2018	novel P2Y12 inhibitors	1997:2018	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	10	2	used	used	2045:2048	arg2	GPIs					1991:1994	GPIs	1991:1994	GPIs	1991:1994	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	6	3	theme	hospitalization	1625:1639	arg1	costs					1641:1645	hospitalization costs	1625:1645	hospitalization costs	1625:1645	Besides, we assessed angina-related health status at 30 days, the length of hospital stay, and hospitalization costs.					
34321166	2	4	theme	novel	638:642	arg1	inhibitors					650:659	novel P2Y12 inhibitors	638:659	novel P2Y12 inhibitors	638:659	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	10	5	theme	novel	1997:2001	arg1	inhibitors					2009:2018	novel P2Y12 inhibitors	1997:2018	novel P2Y12 inhibitors	1997:2018	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	7	6	used	used	1680:1683	arg2	model					1670:1674	A logistic regression model	1648:1674	A logistic regression model	1648:1674	A logistic regression model was used to adjust for baseline differences.					
34321166	2	7	gly	glycoprotein	677:688	arg1	glycoprotein					677:688	glycoprotein IIb/IIIa inhibitors	677:708	glycoprotein IIb/IIIa inhibitors (GPIs)	677:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	4	8	dep	January	1060:1066	arg1	September					1071:1079	September	1071:1079	September	1071:1079	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	4	8	dep	January	1060:1066	arg1	to					1068:1069	to	1068:1069	to	1068:1069	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	5	9	theme	cardiovascular	1394:1407	arg1	events					1409:1414	major adverse cardiovascular events	1380:1414	major adverse cardiovascular events [MACEs	1380:1421	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	9	10	dep	patients	1898:1905	arg1	361					1908:1910	361	1908:1910	361	1908:1910	RESULTS From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study.					
34321166	9	10	dep	patients	1898:1905	arg1	462					1941:1943	462	1941:1943	462	1941:1943	RESULTS From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study.					
34321166	1	11	theme	unfractionated	211:224	arg1	heparin					226:232	unfractionated heparin	211:232	unfractionated heparin (UFH)	211:238	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	1	11	theme	unfractionated	211:224	arg1	UFH					235:237	UFH	235:237	UFH	235:237	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	14	12	theme	shorter	2646:2652	arg1	stays					2663:2667	shorter hospital stays	2646:2667	shorter hospital stays	2646:2667	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	2	13	theme	radial-artery	613:625	arg1	access					627:632	radial-artery access	613:632	radial-artery access	613:632	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	4	14	theme	consecutive	932:942	arg1	patients					963:970	All consecutive high-bleeding-risk patients	928:970	All consecutive high-bleeding-risk patients	928:970	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	14	15	theme	hospitalization	2680:2694	arg1	costs					2696:2700	lower hospitalization costs	2674:2700	lower hospitalization costs	2674:2700	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	11	16	theme	significant	2184:2194	arg1	reduction					2196:2204	significant reduction	2184:2204	significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH	2184:2292	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	0	17	theme	contemporary	147:158	arg1	practice					160:167	contemporary practice	147:167	contemporary practice	147:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	1	18	theme	coronary	426:433	arg1	ACS					445:447	ACS	445:447	ACS	445:447	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	1	18	theme	coronary	426:433	arg1	syndrome					435:442	acute coronary syndrome	420:442	acute coronary syndrome (ACS)	420:448	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	6	19	theme	health	1566:1571	arg1	status					1573:1578	angina-related health status	1551:1578	angina-related health status	1551:1578	Besides, we assessed angina-related health status at 30 days, the length of hospital stay, and hospitalization costs.					
34321166	2	20	theme	access	627:632	arg1	use					606:608	frequent use	597:608	frequent use of radial-artery access and novel P2Y12 inhibitors	597:659	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	2	20	theme	access	627:632	arg1	use					670:672	low use	666:672	low use of glycoprotein IIb/IIIa inhibitors (GPIs)	666:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	2	21	from	risk	528:531	arg1	patients					511:518	patients	511:518	patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs)	511:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	5	22	from	days	1301:1304	arg1	outcomes					1286:1293	the secondary outcomes	1272:1293	the secondary outcomes at 30 days	1272:1304	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	0	23	from	syndrome	135:142	arg1	practice					160:167	contemporary practice	147:167	contemporary practice	147:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	9	24	from	January	1867:1873	arg1	RESULTS					1854:1860	RESULTS	1854:1860	RESULTS From January to September 2019	1854:1891	RESULTS From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study.					
34321166	9	24	from	January	1867:1873	arg1	patients					1898:1905	823 patients	1894:1905	823 patients (361 treated with bivalirudin and 462 treated with UFH)	1894:1961	RESULTS From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study.					
34321166	1	25	theme	BACKGROUND	170:179	arg1	Bivalirudin					181:191	BACKGROUND Bivalirudin	170:191	BACKGROUND Bivalirudin	170:191	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	1	26	dep	complications	275:287	arg1	profile					316:322	a better safety profile	300:322	a better safety profile	300:322	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	3	27	theme	bivalirudin	800:810	arg1	safety					790:795	safety	790:795	safety	790:795	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	3	27	theme	bivalirudin	800:810	arg1	efficacy					777:784	efficacy	777:784	efficacy	777:784	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	13	28	theme	MACEs	2500:2504	arg1	rates					2481:2485	the rates	2477:2485	the rates of NACEs and MACEs in the 15 prespecified subgroups	2477:2537	Furthermore, bivalirudin consistently reduced the rates of NACEs and MACEs in the 15 prespecified subgroups compared with UFH.					
34321166	3	29	dep	aimed	757:761	arg1	AIM					729:731	AIM	729:731	AIM OF THE STUDY	729:744	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	8	30	theme	effect	1750:1755	arg1	Consistency					1721:1731	Consistency	1721:1731	Consistency of the treatment effect of bivalirudin for NACEs and MACEs compared with UFH	1721:1808	Consistency of the treatment effect of bivalirudin for NACEs and MACEs compared with UFH was evaluated in 15 prespecified subgroups.					
34321166	3	31	theme	current	888:894	arg1	practice					896:903	current practice	888:903	current practice	888:903	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	2	32	theme	high	523:526	arg1	risk					528:531	high risk	523:531	high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs)	523:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	3	33	theme	bleeding	838:845	arg1	patients					852:859	high bleeding risk patients	833:859	high bleeding risk patients with ACS undergoing PCI in current practice	833:903	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	15	34	with	patients	2803:2810	arg1	ACS					2817:2819	ACS	2817:2819	ACS undergoing PCI at high risk of bleeding in contemporary practice	2817:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	8	35	theme	bivalirudin	1760:1770	arg1	effect					1750:1755	the treatment effect	1736:1755	the treatment effect of bivalirudin	1736:1770	Consistency of the treatment effect of bivalirudin for NACEs and MACEs compared with UFH was evaluated in 15 prespecified subgroups.					
34321166	7	36	theme	logistic	1650:1657	arg1	model					1670:1674	A logistic regression model	1648:1674	A logistic regression model	1648:1674	A logistic regression model was used to adjust for baseline differences.					
34321166	2	37	theme	contemporary	558:569	arg1	practice					571:578	contemporary practice	558:578	contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs)	558:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	5	38	theme	vessel	1472:1477	arg1	TVR					1498:1500	TVR	1498:1500	TVR	1498:1500	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	38	theme	vessel	1472:1477	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	2	39	theme	IIb/IIIa	690:697	arg1	GPIs					711:714	GPIs	711:714	GPIs	711:714	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	2	39	theme	IIb/IIIa	690:697	arg1	inhibitors					699:708	glycoprotein IIb/IIIa inhibitors	677:708	glycoprotein IIb/IIIa inhibitors (GPIs)	677:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	0	40	theme	acute	120:124	arg1	syndrome					135:142	acute coronary syndrome	120:142	acute coronary syndrome in contemporary practice	120:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	3	41	with	patients	852:859	arg1	ACS					866:868	ACS	866:868	ACS	866:868	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	5	42	theme	myocardial	1330:1339	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	10	43	theme	patients	2086:2093	arg1	%					2065:2065	66.1 %	2060:2065	66.1 %	2060:2065	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	10	43	theme	patients	2086:2093	arg1	%					2077:2077	89.7 %	2072:2077	89.7 %	2072:2077	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	10	43	theme	patients	2086:2093	arg1	patients					2086:2093	the patients	2082:2093	the patients	2082:2093	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	10	43	theme	patients	2086:2093	arg1	%					2057:2057	5.6 %	2053:2057	5.6 %	2053:2057	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	5	44	theme	adverse	1234:1240	arg1	infarction					1190:1199	myocardial infarction	1179:1199	myocardial infarction	1179:1199	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	44	theme	adverse	1234:1240	arg1	[NACEs					1258:1263	net adverse clinical events [NACEs]	1230:1264	net adverse clinical events [NACEs]	1230:1264	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	14	45	theme	improved	2607:2614	arg1	status					2638:2643	improved angina-related health status	2607:2643	improved angina-related health status	2607:2643	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	5	46	theme	primary	1448:1454	arg1	outcome					1456:1462	the primary outcome	1444:1462	the primary outcome	1444:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	47	theme	all-cause	1202:1210	arg1	death					1212:1216	all-cause death	1202:1216	all-cause death	1202:1216	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	47	theme	all-cause	1202:1210	arg1	bleeding					1169:1176	bleeding	1169:1176	bleeding	1169:1176	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	12	48	theme	individual	2299:2308	arg1	endpoints					2310:2318	The individual endpoints	2295:2318	The individual endpoints of death, stroke, ST and TVR	2295:2347	The individual endpoints of death, stroke, ST and TVR did not differ significantly between the 2 groups after adjusting for covariates.					
34321166	5	49	theme	stent	1507:1511	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	1	50	dep	Bivalirudin	181:191	arg1	compared					197:204	compared	197:204	compared with unfractionated heparin (UFH)	197:238	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	0	51	theme	unfractionated	19:32	arg1	heparin					34:40	unfractionated heparin	19:40	unfractionated heparin	19:40	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	1	52	theme	safety	309:314	arg1	profile					316:322	a better safety profile	300:322	a better safety profile	300:322	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	14	53	theme	bivalirudin	2576:2586	arg1	benefits					2564:2571	These benefits	2558:2571	These benefits of bivalirudin	2558:2586	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	15	54	theme	high	2839:2842	arg1	risk					2844:2847	high risk	2839:2847	high risk of bleeding in contemporary practice	2839:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	0	55	theme	coronary	62:69	arg1	intervention					71:82	percutaneous coronary intervention	49:82	percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice	49:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	4	56	from	Hospital	1022:1029	arg1	January					1060:1066	January	1060:1066	January	1060:1066	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	1	57	theme	percutaneous	375:386	arg1	intervention					397:408	percutaneous coronary intervention	375:408	percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS)	375:448	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	1	57	theme	percutaneous	375:386	arg1	PCI					411:413	PCI	411:413	PCI	411:413	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	15	58	from	risk	2844:2847	arg1	practice					2877:2884	contemporary practice	2864:2884	contemporary practice	2864:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	5	59	theme	adverse	1386:1392	arg1	events					1409:1414	major adverse cardiovascular events	1380:1414	major adverse cardiovascular events [MACEs	1380:1421	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	11	60	from	days	2271:2274	arg1	NACEs					2209:2213	NACEs	2209:2213	NACEs	2209:2213	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	60	from	days	2271:2274	arg1	reduction					2196:2204	significant reduction	2184:2204	significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH	2184:2292	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	60	from	days	2271:2274	arg1	infarction					2254:2263	myocardial infarction	2243:2263	myocardial infarction at 30 days compared with UFH	2243:2292	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	60	from	days	2271:2274	arg1	MACEs					2216:2220	MACEs	2216:2220	MACEs	2216:2220	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	5	61	theme	clinical	1242:1249	arg1	infarction					1190:1199	myocardial infarction	1179:1199	myocardial infarction	1179:1199	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	61	theme	clinical	1242:1249	arg1	[NACEs					1258:1263	net adverse clinical events [NACEs]	1230:1264	net adverse clinical events [NACEs]	1230:1264	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	15	62	from	bleeding	2852:2859	arg1	practice					2877:2884	contemporary practice	2864:2884	contemporary practice	2864:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	11	63	theme	baseline	2130:2137	arg1	differences					2139:2149	baseline differences	2130:2149	baseline differences	2130:2149	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	15	64	dep	CONCLUSIONS	2703:2713	arg1	showed					2744:2749	showed	2744:2749	showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice	2744:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	0	65	dep	Bivalirudin	0:10	arg1	intervention					71:82	percutaneous coronary intervention	49:82	percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice	49:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	5	66	theme	myocardial	1179:1188	arg1	infarction					1190:1199	myocardial infarction	1179:1199	myocardial infarction	1179:1199	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	66	theme	myocardial	1179:1188	arg1	[NACEs					1258:1263	net adverse clinical events [NACEs]	1230:1264	net adverse clinical events [NACEs]	1230:1264	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	66	theme	myocardial	1179:1188	arg1	bleeding					1169:1176	bleeding	1169:1176	bleeding	1169:1176	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	4	67	theme	First	1005:1009	arg1	Hospital					1022:1029	the First Affiliated Hospital	1001:1029	the First Affiliated Hospital of Zhengzhou University from January to September 2019	1001:1084	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	5	68	theme	all-cause	1363:1371	arg1	[MACEs					1416:1421	major adverse cardiovascular events [MACEs	1380:1421	major adverse cardiovascular events [MACEs	1380:1421	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	68	theme	all-cause	1363:1371	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	1	69	theme	bleeding	266:273	arg1	complications					275:287	bleeding complications	266:287	bleeding complications	266:287	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	5	70	dep	thrombosis	1513:1522	arg1	ST					1525:1526	ST	1525:1526	ST	1525:1526	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	2	71	theme	P2Y12	644:648	arg1	inhibitors					650:659	novel P2Y12 inhibitors	638:659	novel P2Y12 inhibitors	638:659	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	10	72	theme	P2Y12	2003:2007	arg1	inhibitors					2009:2018	novel P2Y12 inhibitors	1997:2018	novel P2Y12 inhibitors	1997:2018	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	0	73	with	patients	106:113	arg1	syndrome					135:142	acute coronary syndrome	120:142	acute coronary syndrome in contemporary practice	120:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	5	74	theme	net	1230:1232	arg1	infarction					1190:1199	myocardial infarction	1179:1199	myocardial infarction	1179:1199	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	74	theme	net	1230:1232	arg1	[NACEs					1258:1263	net adverse clinical events [NACEs]	1230:1264	net adverse clinical events [NACEs]	1230:1264	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	12	75	theme	TVR	2345:2347	arg1	endpoints					2310:2318	The individual endpoints	2295:2318	The individual endpoints of death, stroke, ST and TVR	2295:2347	The individual endpoints of death, stroke, ST and TVR did not differ significantly between the 2 groups after adjusting for covariates.					
34321166	8	76	theme	prespecified	1830:1841	arg1	subgroups					1843:1851	15 prespecified subgroups	1827:1851	15 prespecified subgroups	1827:1851	Consistency of the treatment effect of bivalirudin for NACEs and MACEs compared with UFH was evaluated in 15 prespecified subgroups.					
34321166	5	77	theme	component	1431:1439	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	77	theme	component	1431:1439	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	77	theme	component	1431:1439	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	77	theme	component	1431:1439	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	77	theme	component	1431:1439	arg1	composite					1317:1325	a composite	1315:1325	a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST)	1315:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	77	theme	component	1431:1439	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	77	theme	component	1431:1439	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	2	78	theme	low	666:668	arg1	use					670:672	low use	666:672	low use of glycoprotein IIb/IIIa inhibitors (GPIs)	666:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	10	79	theme	radial	2025:2030	arg1	approach					2032:2039	radial approach	2025:2039	radial approach	2025:2039	GPIs, novel P2Y12 inhibitors, and radial approach was used in 5.6 %, 66.1 %, and 89.7 % of the patients, respectively.					
34321166	3	80	theme	STUDY	740:744	arg1	AIM					729:731	AIM	729:731	AIM OF THE STUDY	729:744	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	5	81	theme	events	1409:1414	arg1	[MACEs					1416:1421	major adverse cardiovascular events [MACEs	1380:1421	major adverse cardiovascular events [MACEs	1380:1421	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	81	theme	events	1409:1414	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	11	82	from	reduction	2196:2204	arg1	NACEs					2209:2213	NACEs	2209:2213	NACEs	2209:2213	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	82	from	reduction	2196:2204	arg1	infarction					2254:2263	myocardial infarction	2243:2263	myocardial infarction at 30 days compared with UFH	2243:2292	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	82	from	reduction	2196:2204	arg1	days					2271:2274	30 days	2268:2274	30 days compared with UFH	2268:2292	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	82	from	reduction	2196:2204	arg1	MACEs					2216:2220	MACEs	2216:2220	MACEs	2216:2220	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	11	82	from	reduction	2196:2204	arg1	bleeding					2229:2236	bleeding	2229:2236	bleeding	2229:2236	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	7	83	theme	baseline	1699:1706	arg1	differences					1708:1718	baseline differences	1699:1718	baseline differences	1699:1718	A logistic regression model was used to adjust for baseline differences.					
34321166	12	84	theme	stroke	2330:2335	arg1	endpoints					2310:2318	The individual endpoints	2295:2318	The individual endpoints of death, stroke, ST and TVR	2295:2347	The individual endpoints of death, stroke, ST and TVR did not differ significantly between the 2 groups after adjusting for covariates.					
34321166	11	85	theme	myocardial	2243:2252	arg1	infarction					2254:2263	myocardial infarction	2243:2263	myocardial infarction at 30 days compared with UFH	2243:2292	After adjusting for baseline differences, bivalirudin was associated with significant reduction in NACEs, MACEs, major bleeding, and myocardial infarction at 30 days compared with UFH.					
34321166	14	86	theme	lower	2674:2678	arg1	costs					2696:2700	lower hospitalization costs	2674:2700	lower hospitalization costs	2674:2700	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	12	87	theme	ST	2338:2339	arg1	endpoints					2310:2318	The individual endpoints	2295:2318	The individual endpoints of death, stroke, ST and TVR	2295:2347	The individual endpoints of death, stroke, ST and TVR did not differ significantly between the 2 groups after adjusting for covariates.					
34321166	4	88	theme	high-bleeding-risk	944:961	arg1	patients					963:970	All consecutive high-bleeding-risk patients	928:970	All consecutive high-bleeding-risk patients	928:970	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	5	89	theme	30-day	1121:1126	arg1	outcome					1136:1142	The 30-day primary outcome	1117:1142	The 30-day primary outcome	1117:1142	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	89	theme	30-day	1121:1126	arg1	composite					1150:1158	a composite	1148:1158	a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs])	1148:1265	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	89	theme	30-day	1121:1126	arg1	bleeding					1169:1176	bleeding	1169:1176	bleeding	1169:1176	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	6	90	theme	angina-related	1551:1564	arg1	status					1573:1578	angina-related health status	1551:1578	angina-related health status	1551:1578	Besides, we assessed angina-related health status at 30 days, the length of hospital stay, and hospitalization costs.					
34321166	2	91	theme	frequent	597:604	arg1	use					606:608	frequent use	597:608	frequent use of radial-artery access and novel P2Y12 inhibitors	597:659	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	14	92	theme	hospital	2654:2661	arg1	stays					2663:2667	shorter hospital stays	2646:2667	shorter hospital stays	2646:2667	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	5	93	theme	secondary	1276:1284	arg1	outcomes					1286:1293	the secondary outcomes	1272:1293	the secondary outcomes at 30 days	1272:1304	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	15	94	theme	bivalirudin	2732:2742	arg1	treatment					2719:2727	The treatment	2715:2727	The treatment of bivalirudin	2715:2742	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	13	95	theme	prespecified	2516:2527	arg1	subgroups					2529:2537	the 15 prespecified subgroups	2509:2537	the 15 prespecified subgroups	2509:2537	Furthermore, bivalirudin consistently reduced the rates of NACEs and MACEs in the 15 prespecified subgroups compared with UFH.					
34321166	0	96	theme	high-bleeding-risk	87:104	arg1	patients					106:113	high-bleeding-risk patients	87:113	high-bleeding-risk patients with acute coronary syndrome in contemporary practice	87:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	1	97	theme	previous	458:465	arg1	studies					467:473	some previous studies	453:473	some previous studies	453:473	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	14	98	theme	health	2631:2636	arg1	status					2638:2643	improved angina-related health status	2607:2643	improved angina-related health status	2607:2643	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	4	99	dep	MATERIALS	906:914	arg1	patients					963:970	All consecutive high-bleeding-risk patients	928:970	All consecutive high-bleeding-risk patients	928:970	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	1	100	theme	coronary	388:395	arg1	intervention					397:408	percutaneous coronary intervention	375:408	percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS)	375:448	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	1	100	theme	coronary	388:395	arg1	PCI					411:413	PCI	411:413	PCI	411:413	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	8	101	theme	treatment	1740:1748	arg1	effect					1750:1755	the treatment effect	1736:1755	the treatment effect of bivalirudin	1736:1770	Consistency of the treatment effect of bivalirudin for NACEs and MACEs compared with UFH was evaluated in 15 prespecified subgroups.					
34321166	15	102	dep	better	2751:2756	arg1	safety					2771:2776	safety	2771:2776	safety	2771:2776	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	15	102	dep	better	2751:2756	arg1	efficacy					2758:2765	efficacy	2758:2765	efficacy	2758:2765	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	3	103	dep	efficacy	777:784	arg1	the					773:775	the	773:775	the	773:775	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	5	104	theme	target	1465:1470	arg1	TVR					1498:1500	TVR	1498:1500	TVR	1498:1500	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	104	theme	target	1465:1470	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	2	105	theme	inhibitors	699:708	arg1	use					606:608	frequent use	597:608	frequent use of radial-artery access and novel P2Y12 inhibitors	597:659	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	2	105	theme	inhibitors	699:708	arg1	use					670:672	low use	666:672	low use of glycoprotein IIb/IIIa inhibitors (GPIs)	666:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	3	106	theme	risk	847:850	arg1	patients					852:859	high bleeding risk patients	833:859	high bleeding risk patients with ACS undergoing PCI in current practice	833:903	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	0	107	theme	coronary	126:133	arg1	syndrome					135:142	acute coronary syndrome	120:142	acute coronary syndrome in contemporary practice	120:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	5	108	theme	bleeding	1169:1176	arg1	outcome					1136:1142	The 30-day primary outcome	1117:1142	The 30-day primary outcome	1117:1142	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	108	theme	bleeding	1169:1176	arg1	composite					1150:1158	a composite	1148:1158	a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs])	1148:1265	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	108	theme	bleeding	1169:1176	arg1	bleeding					1169:1176	bleeding	1169:1176	bleeding	1169:1176	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	4	109	from	January	1060:1066	arg1	Hospital					1022:1029	the First Affiliated Hospital	1001:1029	the First Affiliated Hospital of Zhengzhou University from January to September 2019	1001:1084	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	7	110	theme	regression	1659:1668	arg1	model					1670:1674	A logistic regression model	1648:1674	A logistic regression model	1648:1674	A logistic regression model was used to adjust for baseline differences.					
34321166	0	111	from	intervention	71:82	arg1	patients					106:113	high-bleeding-risk patients	87:113	high-bleeding-risk patients with acute coronary syndrome in contemporary practice	87:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	5	112	theme	revascularization	1479:1495	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	112	theme	revascularization	1479:1495	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	112	theme	revascularization	1479:1495	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	112	theme	revascularization	1479:1495	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	112	theme	revascularization	1479:1495	arg1	composite					1317:1325	a composite	1315:1325	a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST)	1315:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	112	theme	revascularization	1479:1495	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	112	theme	revascularization	1479:1495	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	2	113	theme	glycoprotein	677:688	arg1	GPIs					711:714	GPIs	711:714	GPIs	711:714	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	2	113	theme	glycoprotein	677:688	arg1	inhibitors					699:708	glycoprotein IIb/IIIa inhibitors	677:708	glycoprotein IIb/IIIa inhibitors (GPIs)	677:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	3	114	theme	high	833:836	arg1	patients					852:859	high bleeding risk patients	833:859	high bleeding risk patients with ACS undergoing PCI in current practice	833:903	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	14	115	theme	angina-related	2616:2629	arg1	status					2638:2643	improved angina-related health status	2607:2643	improved angina-related health status	2607:2643	These benefits of bivalirudin can translate into improved angina-related health status, shorter hospital stays, and lower hospitalization costs.					
34321166	2	116	theme	bleeding	536:543	arg1	risk					528:531	high risk	523:531	high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs)	523:715	Whether this advantage persists in patients at high risk of bleeding according to contemporary practice characterized by frequent use of radial-artery access and novel P2Y12 inhibitors, and low use of glycoprotein IIb/IIIa inhibitors (GPIs) is unclear.					
34321166	5	117	theme	infarction	1341:1350	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	117	theme	infarction	1341:1350	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	117	theme	infarction	1341:1350	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	117	theme	infarction	1341:1350	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	117	theme	infarction	1341:1350	arg1	composite					1317:1325	a composite	1315:1325	a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST)	1315:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	117	theme	infarction	1341:1350	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	117	theme	infarction	1341:1350	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	1	118	theme	acute	420:424	arg1	ACS					445:447	ACS	445:447	ACS	445:447	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	1	118	theme	acute	420:424	arg1	syndrome					435:442	acute coronary syndrome	420:442	acute coronary syndrome (ACS)	420:448	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	15	119	theme	bleeding	2852:2859	arg1	risk					2844:2847	high risk	2839:2847	high risk of bleeding in contemporary practice	2839:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
34321166	4	120	theme	University	1044:1053	arg1	Hospital					1022:1029	the First Affiliated Hospital	1001:1029	the First Affiliated Hospital of Zhengzhou University from January to September 2019	1001:1084	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	5	121	theme	outcome	1456:1462	arg1	[MACEs					1416:1421	major adverse cardiovascular events [MACEs	1380:1421	major adverse cardiovascular events [MACEs	1380:1421	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	TVR					1498:1500	TVR	1498:1500	TVR	1498:1500	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	121	theme	outcome	1456:1462	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	1	122	theme	better	302:307	arg1	profile					316:322	a better safety profile	300:322	a better safety profile	300:322	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	12	123	theme	death	2323:2327	arg1	endpoints					2310:2318	The individual endpoints	2295:2318	The individual endpoints of death, stroke, ST and TVR	2295:2347	The individual endpoints of death, stroke, ST and TVR did not differ significantly between the 2 groups after adjusting for covariates.					
34321166	5	124	theme	thrombosis	1513:1522	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	124	theme	thrombosis	1513:1522	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	124	theme	thrombosis	1513:1522	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	124	theme	thrombosis	1513:1522	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	124	theme	thrombosis	1513:1522	arg1	composite					1317:1325	a composite	1315:1325	a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST)	1315:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	124	theme	thrombosis	1513:1522	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	124	theme	thrombosis	1513:1522	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	0	125	theme	percutaneous	49:60	arg1	intervention					71:82	percutaneous coronary intervention	49:82	percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice	49:167	Bivalirudin versus unfractionated heparin during percutaneous coronary intervention in high-bleeding-risk patients with acute coronary syndrome in contemporary practice.					
34321166	4	126	theme	Affiliated	1011:1020	arg1	Hospital					1022:1029	the First Affiliated Hospital	1001:1029	the First Affiliated Hospital of Zhengzhou University from January to September 2019	1001:1084	MATERIALS AND METHODS All consecutive high-bleeding-risk patients who underwent PCI for ACS at the First Affiliated Hospital of Zhengzhou University from January to September 2019 were retrospectively analyzed.					
34321166	5	127	theme	death	1373:1377	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	127	theme	death	1373:1377	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	127	theme	death	1373:1377	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	127	theme	death	1373:1377	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	127	theme	death	1373:1377	arg1	composite					1317:1325	a composite	1315:1325	a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST)	1315:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	127	theme	death	1373:1377	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	127	theme	death	1373:1377	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	13	128	theme	NACEs	2490:2494	arg1	rates					2481:2485	the rates	2477:2485	the rates of NACEs and MACEs in the 15 prespecified subgroups	2477:2537	Furthermore, bivalirudin consistently reduced the rates of NACEs and MACEs in the 15 prespecified subgroups compared with UFH.					
34321166	1	129	theme	low/medium-bleeding-risk	330:353	arg1	patients					355:362	low/medium-bleeding-risk patients	330:362	low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies	330:473	BACKGROUND Bivalirudin, as compared with unfractionated heparin (UFH), has been shown to reduce bleeding complications and supply a better safety profile among low/medium-bleeding-risk patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndrome (ACS) in some previous studies.					
34321166	5	130	theme	primary	1128:1134	arg1	outcome					1136:1142	The 30-day primary outcome	1117:1142	The 30-day primary outcome	1117:1142	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	130	theme	primary	1128:1134	arg1	composite					1150:1158	a composite	1148:1158	a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs])	1148:1265	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	130	theme	primary	1128:1134	arg1	bleeding					1169:1176	bleeding	1169:1176	bleeding	1169:1176	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	13	131	from	rates	2481:2485	arg1	subgroups					2529:2537	the 15 prespecified subgroups	2509:2537	the 15 prespecified subgroups	2509:2537	Furthermore, bivalirudin consistently reduced the rates of NACEs and MACEs in the 15 prespecified subgroups compared with UFH.					
34321166	5	132	theme	major	1380:1384	arg1	events					1409:1414	major adverse cardiovascular events	1380:1414	major adverse cardiovascular events [MACEs	1380:1421	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	3	133	from	UFH	826:828	arg1	patients					852:859	high bleeding risk patients	833:859	high bleeding risk patients with ACS undergoing PCI in current practice	833:903	AIM OF THE STUDY This study aimed to assess the efficacy and safety of bivalirudin compared with UFH in high bleeding risk patients with ACS undergoing PCI in current practice.					
34321166	9	134	dep	January	1867:1873	arg1	to					1875:1876	to	1875:1876	to	1875:1876	RESULTS From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study.					
34321166	9	134	dep	January	1867:1873	arg1	September					1878:1886	September	1878:1886	September	1878:1886	RESULTS From January to September 2019, 823 patients (361 treated with bivalirudin and 462 treated with UFH) were enrolled in the study.					
34321166	5	135	theme	stoke	1353:1357	arg1	thrombosis					1513:1522	stent thrombosis	1507:1522	stent thrombosis (ST)	1507:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	135	theme	stoke	1353:1357	arg1	infarction					1341:1350	myocardial infarction	1330:1350	myocardial infarction	1330:1350	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	135	theme	stoke	1353:1357	arg1	death					1373:1377	all-cause death	1363:1377	all-cause death (major adverse cardiovascular events [MACEs])	1363:1423	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	135	theme	stoke	1353:1357	arg1	stoke					1353:1357	stoke	1353:1357	stoke	1353:1357	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	135	theme	stoke	1353:1357	arg1	composite					1317:1325	a composite	1315:1325	a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST)	1315:1527	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	135	theme	stoke	1353:1357	arg1	component					1431:1439	each component	1426:1439	each component of the primary outcome	1426:1462	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	135	theme	stoke	1353:1357	arg1	revascularization					1479:1495	target vessel revascularization	1465:1495	target vessel revascularization (TVR)	1465:1501	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	136	theme	events	1251:1256	arg1	infarction					1190:1199	myocardial infarction	1179:1199	myocardial infarction	1179:1199	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	5	136	theme	events	1251:1256	arg1	[NACEs					1258:1263	net adverse clinical events [NACEs]	1230:1264	net adverse clinical events [NACEs]	1230:1264	The 30-day primary outcome was a composite of major bleeding, myocardial infarction, all-cause death, or stroke (net adverse clinical events [NACEs]), and the secondary outcomes at 30 days included a composite of myocardial infarction, stoke, or all-cause death (major adverse cardiovascular events [MACEs]), each component of the primary outcome, target vessel revascularization (TVR) and stent thrombosis (ST).					
34321166	15	137	theme	contemporary	2864:2875	arg1	practice					2877:2884	contemporary practice	2864:2884	contemporary practice	2864:2884	CONCLUSIONS The treatment of bivalirudin showed better efficacy and safety as compared to UFH among patients with ACS undergoing PCI at high risk of bleeding in contemporary practice.					
32829293	2	0	theme	acid	505:508	arg1	composition					510:520	fatty acid composition	499:520	fatty acid composition	499:520	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	5	1	theme	fish	727:730	arg1	samples					732:738	fish samples	727:738	fish samples	727:738	No significant effects on the oxidation of fish samples were observed with TBARS increasing from 12.04 to 22.50 mg/kg.					
32829293	6	2	theme	microorganisms	849:862	arg1	growth					839:844	the growth	835:844	the growth of microorganisms	835:862	Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed.					
32829293	4	3	theme	mMol	667:670	arg1	FRAP					657:660	high FRAP	652:660	high FRAP (6.6 mMol Trolox/mg)	652:681	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	4	3	theme	mMol	667:670	arg1	Trolox/mg					672:680	6.6 mMol Trolox/mg	663:680	6.6 mMol Trolox/mg	663:680	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	0	4	theme	Ala-Tyr	86:92	arg1	peptide					94:100	Ala-Tyr peptide	86:100	Ala-Tyr peptide	86:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	0	5	from	effects	4:10	arg1	Atlantic					111:118	fresh Atlantic	105:118	fresh Atlantic	105:118	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	1	6	theme	first	159:163	arg1	time					165:168	the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties	155:314	the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties	155:314	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	1	6	theme	first	159:163	arg1	This					147:150	This	147:150	This	147:150	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	0	7	theme	fresh	105:109	arg1	Atlantic					111:118	fresh Atlantic	105:118	fresh Atlantic	105:118	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	0	8	dep	mackerel	120:127	arg1	stored					129:134	stored	129:134	mackerel stored at -18 °C	120:144	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	8	9	theme	Antimicrobiological	1020:1038	arg1	properties					1040:1049	Antimicrobiological properties	1020:1049	Antimicrobiological properties of the film	1020:1061	Antimicrobiological properties of the film should be further investigated during storage of perishable food products at temperatures above 0 °C.					
32829293	8	10	theme	products	1128:1135	arg1	storage					1101:1107	storage	1101:1107	storage of perishable food products	1101:1135	Antimicrobiological properties of the film should be further investigated during storage of perishable food products at temperatures above 0 °C.					
32829293	5	11	theme	samples	732:738	arg1	oxidation					714:722	the oxidation	710:722	the oxidation of fish samples	710:738	No significant effects on the oxidation of fish samples were observed with TBARS increasing from 12.04 to 22.50 mg/kg.					
32829293	0	12	with	system	74:79	arg1	peptide					94:100	Ala-Tyr peptide	86:100	Ala-Tyr peptide	86:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	4	13	theme	high	652:655	arg1	FRAP					657:660	high FRAP	652:660	high FRAP (6.6 mMol Trolox/mg)	652:681	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	4	13	theme	high	652:655	arg1	Trolox/mg					672:680	6.6 mMol Trolox/mg	663:680	6.6 mMol Trolox/mg	663:680	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	2	14	theme	biogenic	475:482	arg1	content					490:496	biogenic amine content	475:496	biogenic amine content	475:496	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	3	15	theme	higher	550:555	arg1	TS					557:558	higher TS	550:558	higher TS (13.4 MPa)	550:569	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	3	15	theme	higher	550:555	arg1	13.4 MPa					561:568	13.4 MPa	561:568	13.4 MPa	561:568	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	4	16	theme	scavenging	626:635	arg1	properties					637:646	radical scavenging properties	618:646	radical scavenging properties	618:646	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	2	17	theme	fatty	499:503	arg1	composition					510:520	fatty acid composition	499:520	fatty acid composition	499:520	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	1	18	theme	active	175:180	arg1	films					229:233	active double-layered furcellaran/gelatin hydrolysate films	175:233	active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide	175:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	6	19	theme	microbiological	886:900	arg1	growth					902:907	microbiological growth	886:907	microbiological growth	886:907	Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed.					
32829293	8	20	theme	film	1058:1061	arg1	properties					1040:1049	Antimicrobiological properties	1020:1049	Antimicrobiological properties of the film	1020:1061	Antimicrobiological properties of the film should be further investigated during storage of perishable food products at temperatures above 0 °C.					
32829293	3	21	theme	lower	575:579	arg1	WS					581:582	lower WS	575:582	lower WS (62.8%)	575:590	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	3	21	theme	lower	575:579	arg1	%					589:589	62.8%	585:589	62.8%	585:589	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	5	22	theme	significant	687:697	arg1	effects					699:705	No significant effects	684:705	No significant effects on the oxidation of fish samples	684:738	No significant effects on the oxidation of fish samples were observed with TBARS increasing from 12.04 to 22.50 mg/kg.					
32829293	1	23	theme	double-layered	182:195	arg1	films					229:233	active double-layered furcellaran/gelatin hydrolysate films	175:233	active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide	175:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	0	24	theme	active	15:20	arg1	system					74:79	active double-layered furcellaran/gelatin hydrolysate film system	15:79	active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide	15:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	7	25	theme	films	980:984	arg1	application					965:975	the application	961:975	the application of films	961:984	However, the application of films inhibited the formation of TVB-N.					
32829293	1	26	theme	furcellaran/gelatin	197:215	arg1	films					229:233	active double-layered furcellaran/gelatin hydrolysate films	175:233	active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide	175:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	2	27	from	changes	426:432	arg1	composition					510:520	fatty acid composition	499:520	fatty acid composition	499:520	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	2	27	from	changes	426:432	arg1	TVB-N					468:472	TVB-N	468:472	TVB-N	468:472	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	2	27	from	changes	426:432	arg1	quality					459:465	their microbiological quality	437:465	their microbiological quality	437:465	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	2	27	from	changes	426:432	arg1	content					490:496	biogenic amine content	475:496	biogenic amine content	475:496	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	2	27	from	changes	426:432	arg1	TBARS					526:530	TBARS	526:530	TBARS	526:530	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	5	28	from	effects	699:705	arg1	oxidation					714:722	the oxidation	710:722	the oxidation of fish samples	710:738	No significant effects on the oxidation of fish samples were observed with TBARS increasing from 12.04 to 22.50 mg/kg.					
32829293	1	29	theme	hydrolysate	217:227	arg1	films					229:233	active double-layered furcellaran/gelatin hydrolysate films	175:233	active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide	175:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	0	30	theme	furcellaran/gelatin	37:55	arg1	system					74:79	active double-layered furcellaran/gelatin hydrolysate film system	15:79	active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide	15:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	4	31	theme	radical	618:624	arg1	properties					637:646	radical scavenging properties	618:646	radical scavenging properties	618:646	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	0	32	theme	double-layered	22:35	arg1	system					74:79	active double-layered furcellaran/gelatin hydrolysate film system	15:79	active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide	15:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	4	33	dep	DPPH	613:616	arg1	properties					637:646	radical scavenging properties	618:646	radical scavenging properties	618:646	The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg).					
32829293	2	34	theme	amine	484:488	arg1	content					490:496	biogenic amine content	475:496	biogenic amine content	475:496	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	3	35	contain	had	546:548	arg2	WS					581:582	lower WS	575:582	lower WS (62.8%)	575:590	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	3	35	contain	had	546:548	arg2	TS					557:558	higher TS	550:558	higher TS (13.4 MPa)	550:569	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	3	35	contain	had	546:548	arg2	%					589:589	62.8%	585:589	62.8%	585:589	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	3	35	contain	had	546:548	arg2	13.4 MPa					561:568	13.4 MPa	561:568	13.4 MPa	561:568	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	3	35	contain	had	546:548	arg1	films					540:544	Active films	533:544	Active films	533:544	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	0	36	theme	system	74:79	arg1	effects					4:10	The effects	0:10	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic	0:118	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	8	37	theme	food	1123:1126	arg1	products					1128:1135	perishable food products	1112:1135	perishable food products	1112:1135	Antimicrobiological properties of the film should be further investigated during storage of perishable food products at temperatures above 0 °C.					
32829293	0	38	theme	hydrolysate	57:67	arg1	system					74:79	active double-layered furcellaran/gelatin hydrolysate film system	15:79	active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide	15:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32829293	6	39	theme	amine	921:925	arg1	formation					927:935	biogenic amine formation	912:935	biogenic amine formation	912:935	Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed.					
32829293	1	40	contain	containing	235:244	arg1	films					229:233	active double-layered furcellaran/gelatin hydrolysate films	175:233	active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide	175:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	1	40	contain	containing	235:244	arg2	peptide					254:260	Ala-Tyr peptide	246:260	Ala-Tyr peptide	246:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	6	41	theme	biogenic	912:919	arg1	formation					927:935	biogenic amine formation	912:935	biogenic amine formation	912:935	Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed.					
32829293	7	42	theme	TVB-N	1013:1017	arg1	formation					1000:1008	the formation	996:1008	the formation of TVB-N	996:1017	However, the application of films inhibited the formation of TVB-N.					
32829293	6	43	from	differences	871:881	arg1	formation					927:935	biogenic amine formation	912:935	biogenic amine formation	912:935	Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed.					
32829293	6	43	from	differences	871:881	arg1	growth					902:907	microbiological growth	886:907	microbiological growth	886:907	Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed.					
32829293	1	44	theme	Ala-Tyr	246:252	arg1	peptide					254:260	Ala-Tyr peptide	246:260	Ala-Tyr peptide	246:260	This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties.					
32829293	8	45	theme	perishable	1112:1121	arg1	products					1128:1135	perishable food products	1112:1135	perishable food products	1112:1135	Antimicrobiological properties of the film should be further investigated during storage of perishable food products at temperatures above 0 °C.					
32829293	2	46	theme	Atlantic	348:355	arg1	mackerel					357:364	Atlantic mackerel	348:364	Atlantic mackerel stored at -18 °C for 4 months	348:394	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	2	47	used	used	340:343	arg2	films					329:333	films	329:333	films	329:333	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	2	48	theme	microbiological	443:457	arg1	quality					459:465	their microbiological quality	437:465	their microbiological quality	437:465	Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS.					
32829293	3	49	theme	Active	533:538	arg1	films					540:544	Active films	533:544	Active films	533:544	Active films had higher TS (13.4 MPa) and lower WS (62.8%).					
32829293	0	50	theme	film	69:72	arg1	system					74:79	active double-layered furcellaran/gelatin hydrolysate film system	15:79	active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide	15:100	The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 °C.					
32271757	4	0	theme	realistic	967:975	arg1	environments					993:1004	more realistic plasma membrane environments	962:1004	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	5	1	theme	CD44	1146:1149	arg1	palmitoylations					1127:1141	the palmitoylations	1123:1141	the palmitoylations of CD44	1123:1149	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	6	2	theme	membrane	1292:1299	arg1	FERM					1309:1312	the membrane adaptor FERM	1288:1312	the membrane adaptor FERM	1288:1312	Additionally, we studied the association between CD44 and the membrane adaptor FERM in dependence of these factors.					
32271757	3	3	theme	raft	686:689	arg1	domains					691:697	the lipid raft domains	676:697	the lipid raft domains	676:697	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	3	theme	raft	686:689	arg1	PIP2					762:765	PIP2	762:765	PIP2	762:765	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	4	4	theme	dynamics	842:849	arg1	simulations					851:861	extensive molecular dynamics simulations	822:861	extensive molecular dynamics simulations	822:861	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	5	5	contain	have	1204:1207	arg2	effect					1222:1227	the opposite effect	1209:1227	the opposite effect	1209:1227	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	5	5	contain	have	1204:1207	arg1	lipids					1197:1202	PIP2 lipids	1192:1202	PIP2 lipids	1192:1202	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	4	6	theme	model	935:939	arg1	membranes					941:949	phase separated model membranes	919:949	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	7	7	theme	observed	1442:1449	arg1	mode					1459:1462	an experimentally observed binding mode	1424:1462	an experimentally observed binding mode	1424:1462	We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity.					
32271757	2	8	dep	keeps	368:372	arg1	On					337:338	On	337:338	On	337:338	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	2	8	dep	keeps	368:372	arg1	whereas					454:460	whereas	454:460	whereas	454:460	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	5	9	theme	PIP2	1192:1195	arg1	lipids					1197:1202	PIP2 lipids	1192:1202	PIP2 lipids	1192:1202	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	4	10	theme	separated	925:933	arg1	membranes					941:949	phase separated model membranes	919:949	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	2	11	theme	complex	594:600	arg1	formation					564:572	the formation	560:572	the formation of the CD44-adaptor complex	560:600	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	7	12	theme	binding	1451:1457	arg1	mode					1459:1462	an experimentally observed binding mode	1424:1462	an experimentally observed binding mode	1424:1462	We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity.					
32271757	8	13	theme	protein	1687:1693	arg1	modifications					1695:1707	protein modifications	1687:1707	protein modifications	1687:1707	Together, our results shed light on the sophisticated mechanism on how membrane translocation and peripheral protein association can be controlled by both protein modifications and membrane composition.					
32271757	8	14	theme	membrane	1603:1610	arg1	translocation					1612:1624	membrane translocation	1603:1624	membrane translocation	1603:1624	Together, our results shed light on the sophisticated mechanism on how membrane translocation and peripheral protein association can be controlled by both protein modifications and membrane composition.					
32271757	2	15	theme	phosphatidylinositol-4,5-biphosphate	497:532	arg1	lipids					541:546	phosphatidylinositol-4,5-biphosphate (PIP2) lipids	497:546	phosphatidylinositol-4,5-biphosphate (PIP2) lipids	497:546	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	4	16	theme	extensive	822:830	arg1	simulations					851:861	extensive molecular dynamics simulations	822:861	extensive molecular dynamics simulations	822:861	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	1	17	theme	adaptors	197:204	arg1	crucial					243:249	crucial	243:249	crucial	243:249	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	1	17	theme	adaptors	197:204	arg1	co-localization					117:131	The co-localization	113:131	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments	113:238	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	8	18	theme	sophisticated	1572:1584	arg1	mechanism					1586:1594	the sophisticated mechanism	1568:1594	the sophisticated mechanism	1568:1594	Together, our results shed light on the sophisticated mechanism on how membrane translocation and peripheral protein association can be controlled by both protein modifications and membrane composition.					
32271757	4	19	theme	membrane	984:991	arg1	environments					993:1004	more realistic plasma membrane environments	962:1004	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	4	20	theme	phase	919:923	arg1	membranes					941:949	phase separated model membranes	919:949	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	1	21	theme	cytoplasmic	185:195	arg1	adaptors					197:204	cytoplasmic adaptors	185:204	cytoplasmic adaptors	185:204	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	3	22	from	dependence	707:716	arg1	palmitoylations					726:740	both palmitoylations	721:740	both palmitoylations	721:740	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	5	23	theme	PIP2	1102:1105	arg1	lipids					1107:1112	PIP2 lipids	1102:1112	PIP2 lipids	1102:1112	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	3	24	from	presence	750:757	arg1	palmitoylations					726:740	both palmitoylations	721:740	both palmitoylations	721:740	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	7	25	theme	PIP2	1375:1378	arg1	lipids					1380:1385	PIP2 lipids	1375:1385	PIP2 lipids	1375:1385	We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity.					
32271757	4	26	theme	raft	876:879	arg1	affinity					881:888	the raft affinity and translocation	872:906	affinity	881:888	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	2	27	theme	raft	388:391	arg1	domains					393:399	lipid raft domains	382:399	lipid raft domains	382:399	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	0	28	theme	Molecular	0:8	arg1	mechanism					10:18	Molecular mechanism	0:18	Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2.	0:111	Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2.					
32271757	4	29	theme	molecular	832:840	arg1	simulations					851:861	extensive molecular dynamics simulations	822:861	extensive molecular dynamics simulations	822:861	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	7	30	theme	lipids	1380:1385	arg1	presence					1363:1370	the presence	1359:1370	the presence of PIP2 lipids	1359:1385	We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity.					
32271757	2	31	theme	lipid	382:386	arg1	domains					393:399	lipid raft domains	382:399	lipid raft domains	382:399	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	0	32	theme	bidirectional	24:36	arg1	regulation					38:47	bidirectional regulation	24:47	bidirectional regulation of CD44	24:55	Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2.					
32271757	3	33	dep	domains	691:697	arg1	out					669:671	out	669:671	out	669:671	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	4	34	theme	CD44	911:914	arg1	translocation					894:906	the raft affinity and translocation	872:906	translocation	894:906	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	4	34	theme	CD44	911:914	arg1	affinity					881:888	the raft affinity and translocation	872:906	affinity	881:888	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	1	35	from	co-localization	117:131	arg1	environments					227:238	specific membrane environments	209:238	specific membrane environments	209:238	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	2	36	theme	different	317:325	arg1	pathways					327:334	two different pathways	313:334	two different pathways	313:334	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	8	37	theme	membrane	1713:1720	arg1	composition					1722:1732	membrane composition	1713:1732	membrane composition	1713:1732	Together, our results shed light on the sophisticated mechanism on how membrane translocation and peripheral protein association can be controlled by both protein modifications and membrane composition.					
32271757	1	38	theme	specific	209:216	arg1	environments					227:238	specific membrane environments	209:238	specific membrane environments	209:238	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	1	39	theme	membrane	218:225	arg1	environments					227:238	specific membrane environments	209:238	specific membrane environments	209:238	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	2	40	theme	other	469:473	arg1	hand					475:478	the other hand	465:478	the other hand	465:478	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	5	41	theme	raft	1178:1181	arg1	domains					1183:1189	raft domains	1178:1189	raft domains	1178:1189	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	2	42	theme	lipids	541:546	arg1	presence					485:492	the presence	481:492	the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids	481:546	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	7	43	theme	binding	1514:1520	arg1	affinity					1522:1529	no binding affinity	1511:1529	no binding affinity	1511:1529	We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity.					
32271757	7	44	theme	palmitoylated	1483:1495	arg1	species					1497:1503	the highly palmitoylated species	1472:1503	the highly palmitoylated species	1472:1503	We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity.					
32271757	3	45	from	domains	691:697	arg1	palmitoylations					726:740	both palmitoylations	721:740	both palmitoylations	721:740	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	4	46	theme	plasma	977:982	arg1	environments					993:1004	more realistic plasma membrane environments	962:1004	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	5	47	theme	palmitoylations	1066:1080	arg1	influence					1049:1057	the influence	1045:1057	the influence of the palmitoylations	1045:1080	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	5	47	theme	palmitoylations	1066:1080	arg1	presence					1090:1097	the presence	1086:1097	the presence of PIP2 lipids	1086:1112	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	2	48	theme	cytoplasmic	433:443	arg1	adaptor					445:451	the cytoplasmic adaptor	429:451	the cytoplasmic adaptor	429:451	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	2	49	theme	CD44-adaptor	581:592	arg1	complex					594:600	the CD44-adaptor complex	577:600	the CD44-adaptor complex	577:600	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	2	50	theme	hand	348:351	arg1	palmitoylation					353:366	the one hand palmitoylation	340:366	the one hand palmitoylation	340:366	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	0	51	theme	CD44	52:55	arg1	regulation					38:47	bidirectional regulation	24:47	bidirectional regulation of CD44	24:55	Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2.					
32271757	3	52	theme	PIP2	762:765	arg1	presence					750:757	the presence	746:757	the presence of PIP2	746:765	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	52	theme	PIP2	762:765	arg1	PIP2					762:765	PIP2	762:765	PIP2	762:765	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	52	theme	PIP2	762:765	arg1	domains					691:697	the lipid raft domains	676:697	the lipid raft domains	676:697	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	52	theme	PIP2	762:765	arg1	dependence					707:716	its dependence	703:716	its dependence on both palmitoylations	703:740	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	53	attach	presence	750:757	arg2	PIP2					762:765	PIP2	762:765	PIP2	762:765	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	53	attach	presence	750:757	arg1	palmitoylations					726:740	both palmitoylations	721:740	both palmitoylations	721:740	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	4	54	from	translocation	894:906	arg1	environments					993:1004	more realistic plasma membrane environments	962:1004	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	4	54	from	translocation	894:906	arg1	membranes					941:949	phase separated model membranes	919:949	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	5	55	theme	delicate	1020:1027	arg1	balance					1029:1035	a delicate balance	1018:1035	a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids	1018:1112	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	8	56	theme	peripheral	1630:1639	arg1	association					1649:1659	peripheral protein association	1630:1659	peripheral protein association	1630:1659	Together, our results shed light on the sophisticated mechanism on how membrane translocation and peripheral protein association can be controlled by both protein modifications and membrane composition.					
32271757	2	57	from	CD44	374:377	arg1	domains					393:399	lipid raft domains	382:399	lipid raft domains	382:399	The process is controlled by two different pathways: On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor, whereas on the other hand, the presence of phosphatidylinositol-4,5-biphosphate (PIP2) lipids accelerates the formation of the CD44-adaptor complex.					
32271757	3	58	theme	lipid	680:684	arg1	domains					691:697	the lipid raft domains	676:697	the lipid raft domains	676:697	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	58	theme	lipid	680:684	arg1	PIP2					762:765	PIP2	762:765	PIP2	762:765	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	3	59	theme	molecular	607:615	arg1	mechanism					617:625	The molecular mechanism	603:625	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2	603:765	The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains, however, elusive.					
32271757	6	60	theme	adaptor	1301:1307	arg1	FERM					1309:1312	the membrane adaptor FERM	1288:1312	the membrane adaptor FERM	1288:1312	Additionally, we studied the association between CD44 and the membrane adaptor FERM in dependence of these factors.					
32271757	4	61	from	affinity	881:888	arg1	environments					993:1004	more realistic plasma membrane environments	962:1004	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	4	61	from	affinity	881:888	arg1	membranes					941:949	phase separated model membranes	919:949	phase separated model membranes as well as more realistic plasma membrane environments	919:1004	In this study, we performed extensive molecular dynamics simulations to study the raft affinity and translocation of CD44 in phase separated model membranes as well as more realistic plasma membrane environments.					
32271757	1	62	theme	Cluster-of-Differentiation-44	136:164	arg1	CD44					175:178	CD44	175:178	CD44	175:178	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	1	62	theme	Cluster-of-Differentiation-44	136:164	arg1	protein					166:172	Cluster-of-Differentiation-44 protein	136:172	Cluster-of-Differentiation-44 protein (CD44)	136:179	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	0	63	theme	raft	67:70	arg1	affiliation					72:82	lipid raft affiliation	61:82	lipid raft affiliation by palmitoylations and PIP2	61:110	Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2.					
32271757	1	64	theme	protein	166:172	arg1	crucial					243:249	crucial	243:249	crucial	243:249	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	1	64	theme	protein	166:172	arg1	co-localization					117:131	The co-localization	113:131	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments	113:238	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	8	65	theme	protein	1641:1647	arg1	association					1649:1659	peripheral protein association	1630:1659	peripheral protein association	1630:1659	Together, our results shed light on the sophisticated mechanism on how membrane translocation and peripheral protein association can be controlled by both protein modifications and membrane composition.					
32271757	1	66	theme	cell	255:258	arg1	adhesion					260:267	cell adhesion	255:267	cell adhesion	255:267	The co-localization of Cluster-of-Differentiation-44 protein (CD44) and cytoplasmic adaptors in specific membrane environments is crucial for cell adhesion and migration.					
32271757	0	67	theme	lipid	61:65	arg1	affiliation					72:82	lipid raft affiliation	61:82	lipid raft affiliation by palmitoylations and PIP2	61:110	Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2.					
32271757	5	68	theme	lipids	1107:1112	arg1	influence					1049:1057	the influence	1045:1057	the influence of the palmitoylations	1045:1080	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	5	68	theme	lipids	1107:1112	arg1	presence					1090:1097	the presence	1086:1097	the presence of PIP2 lipids	1086:1112	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	6	69	from	association	1259:1269	arg1	dependence					1317:1326	dependence	1317:1326	dependence of these factors	1317:1343	Additionally, we studied the association between CD44 and the membrane adaptor FERM in dependence of these factors.					
32271757	5	70	theme	opposite	1213:1220	arg1	effect					1222:1227	the opposite effect	1209:1227	the opposite effect	1209:1227	We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids: whereas the palmitoylations of CD44 increases the affinity for raft domains, PIP2 lipids have the opposite effect.					
32271757	6	71	theme	factors	1337:1343	arg1	dependence					1317:1326	dependence	1317:1326	dependence of these factors	1317:1343	Additionally, we studied the association between CD44 and the membrane adaptor FERM in dependence of these factors.					
32219272	5	0	theme	moderate	783:790	arg1	stability					792:800	moderate stability	783:800	moderate stability	783:800	The L-PG films showed negligible cytotoxicity and moderate stability under different conditions but displayed enzyme-mediated degradability.					
32219272	1	1	theme	many	203:206	arg1	applications					208:219	many applications	203:219	many applications in catalysis, bioanalytical sensing and nanomedicine	203:272	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	7	2	theme	silver	1004:1009	arg1	films					1049:1053	gold and silver nanoparticle-loaded L-PG multilayered films	995:1053	films	1049:1053	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	4	3	theme	thin	660:663	arg1	film					665:668	a thin film	658:668	a thin film on substrates of varying chemical composition and wettability	658:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	4	4	from	substrates	673:682	arg1	deposition					644:653	the rapid and conformal deposition	620:653	the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability	620:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	5	5	theme	L-PG	737:740	arg1	films					742:746	The L-PG films	733:746	The L-PG films	733:746	The L-PG films showed negligible cytotoxicity and moderate stability under different conditions but displayed enzyme-mediated degradability.					
32219272	8	6	theme	present	1179:1185	arg1	approach					1187:1194	the present approach	1175:1194	the present approach	1175:1194	Finally, we highlight the versatility of the present approach to engineer multifaceted interfaces for catalysis and sensing applications.					
32219272	4	7	theme	L-PG	595:598	arg1	assembly					583:590	The assembly	579:590	The assembly of L-PG on surfaces	579:610	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	8	8	theme	approach	1187:1194	arg1	versatility					1160:1170	the versatility	1156:1170	the versatility of the present approach to engineer multifaceted interfaces for catalysis and sensing applications	1156:1269	Finally, we highlight the versatility of the present approach to engineer multifaceted interfaces for catalysis and sensing applications.					
32219272	1	9	from	applications	208:219	arg1	useful					193:198	useful	193:198	useful	193:198	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	1	9	from	applications	208:219	arg1	nanomedicine					261:272	nanomedicine	261:272	nanomedicine	261:272	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	1	9	from	applications	208:219	arg1	catalysis					224:232	catalysis	224:232	catalysis	224:232	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	1	9	from	applications	208:219	arg1	sensing					249:255	bioanalytical sensing	235:255	bioanalytical sensing	235:255	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	7	10	theme	gold	995:998	arg1	films					1049:1053	gold and silver nanoparticle-loaded L-PG multilayered films	995:1053	films	1049:1053	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	4	11	from	film	665:668	arg1	substrates					673:682	substrates	673:682	substrates of varying chemical composition and wettability	673:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	4	12	from	assembly	583:590	arg1	surfaces					603:610	surfaces	603:610	surfaces	603:610	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	1	13	from	useful	193:198	arg1	applications					208:219	many applications	203:219	many applications in catalysis, bioanalytical sensing and nanomedicine	203:272	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	1	14	theme	Multifunctional	89:103	arg1	interfaces					145:154	Multifunctional and biodegradable nanostructured hybrid interfaces	89:154	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers	89:175	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	4	15	theme	film	665:668	arg1	deposition					644:653	the rapid and conformal deposition	620:653	the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability	620:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	5	16	theme	enzyme-mediated	843:857	arg1	degradability					859:871	enzyme-mediated degradability	843:871	enzyme-mediated degradability	843:871	The L-PG films showed negligible cytotoxicity and moderate stability under different conditions but displayed enzyme-mediated degradability.					
32219272	4	17	theme	composition	704:714	arg1	substrates					673:682	substrates	673:682	substrates of varying chemical composition and wettability	673:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	2	18	theme	films	335:339	arg1	engineering					297:307	the engineering	293:307	the engineering of multifunctional hybrid films	293:339	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	3	19	theme	amphiphilic	517:527	arg1	nature					529:534	their amphiphilic nature	511:534	their amphiphilic nature	511:534	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	1	20	theme	biodegradable	109:121	arg1	interfaces					145:154	Multifunctional and biodegradable nanostructured hybrid interfaces	89:154	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers	89:175	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	0	21	theme	multifunctional	16:30	arg1	interfaces					39:48	multifunctional hybrid interfaces	16:48	multifunctional hybrid interfaces using adhesive glycogen nanoparticles	16:86	Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles.					
32219272	7	22	with	films	1049:1053	arg1	properties					1108:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	7	23	theme	surface-enhanced	1074:1089	arg1	properties					1108:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	1	24	theme	nanostructured	123:136	arg1	interfaces					145:154	Multifunctional and biodegradable nanostructured hybrid interfaces	89:154	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers	89:175	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	4	25	from	deposition	644:653	arg1	substrates					673:682	substrates	673:682	substrates of varying chemical composition and wettability	673:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	4	26	theme	conformal	634:642	arg1	deposition					644:653	the rapid and conformal deposition	620:653	the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability	620:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	8	27	theme	multifaceted	1208:1219	arg1	interfaces					1221:1230	multifaceted interfaces	1208:1230	multifaceted interfaces for catalysis and sensing applications	1208:1269	Finally, we highlight the versatility of the present approach to engineer multifaceted interfaces for catalysis and sensing applications.					
32219272	3	28	contain	possess	469:475	arg1	blocks					462:467	These nano building blocks	442:467	These nano building blocks	442:467	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	3	28	contain	possess	469:475	arg2	properties					486:495	adhesive properties	477:495	adhesive properties	477:495	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	3	28	contain	possess	469:475	arg2	groups					571:576	reactive functional disulfide groups	541:576	reactive functional disulfide groups	541:576	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	1	29	theme	hybrid	138:143	arg1	interfaces					145:154	Multifunctional and biodegradable nanostructured hybrid interfaces	89:154	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers	89:175	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	1	30	theme	bioanalytical	235:247	arg1	sensing					249:255	bioanalytical sensing	235:255	bioanalytical sensing	235:255	Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many applications in catalysis, bioanalytical sensing and nanomedicine.					
32219272	3	31	theme	nano	448:451	arg1	blocks					462:467	These nano building blocks	442:467	These nano building blocks	442:467	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	7	32	theme	Raman	1091:1095	arg1	properties					1108:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	6	33	theme	metal	887:891	arg1	nanoparticles					893:905	metal nanoparticles	887:905	metal nanoparticles	887:905	In addition, metal nanoparticles were embedded into the L-PG layers to build up multilayered hybrid films.					
32219272	2	34	theme	biological	364:373	arg1	nanoparticles					375:387	adhesive biological nanoparticles	355:387	adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG)	355:439	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	3	35	theme	reactive	541:548	arg1	groups					571:576	reactive functional disulfide groups	541:576	reactive functional disulfide groups	541:576	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	3	36	theme	building	453:460	arg1	blocks					462:467	These nano building blocks	442:467	These nano building blocks	442:467	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	2	37	theme	adhesive	355:362	arg1	nanoparticles					375:387	adhesive biological nanoparticles	355:387	adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG)	355:439	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	7	38	theme	scattering	1097:1106	arg1	properties					1108:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	3	39	theme	functional	550:559	arg1	groups					571:576	reactive functional disulfide groups	541:576	reactive functional disulfide groups	541:576	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	0	40	theme	adhesive	56:63	arg1	nanoparticles					74:86	adhesive glycogen nanoparticles	56:86	adhesive glycogen nanoparticles	56:86	Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles.					
32219272	5	41	theme	different	808:816	arg1	conditions					818:827	different conditions	808:827	different conditions	808:827	The L-PG films showed negligible cytotoxicity and moderate stability under different conditions but displayed enzyme-mediated degradability.					
32219272	6	42	from	embedded	912:919	arg1	addition					877:884	addition	877:884	addition	877:884	In addition, metal nanoparticles were embedded into the L-PG layers to build up multilayered hybrid films.					
32219272	4	43	theme	wettability	720:730	arg1	substrates					673:682	substrates	673:682	substrates of varying chemical composition and wettability	673:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	3	44	theme	adhesive	477:484	arg1	properties					486:495	adhesive properties	477:495	adhesive properties	477:495	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	5	45	theme	negligible	755:764	arg1	cytotoxicity					766:777	negligible cytotoxicity	755:777	negligible cytotoxicity	755:777	The L-PG films showed negligible cytotoxicity and moderate stability under different conditions but displayed enzyme-mediated degradability.					
32219272	6	46	theme	hybrid	967:972	arg1	films					974:978	multilayered hybrid films	954:978	multilayered hybrid films	954:978	In addition, metal nanoparticles were embedded into the L-PG layers to build up multilayered hybrid films.					
32219272	0	47	theme	glycogen	65:72	arg1	nanoparticles					74:86	adhesive glycogen nanoparticles	56:86	adhesive glycogen nanoparticles	56:86	Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles.					
32219272	4	48	theme	rapid	624:628	arg1	deposition					644:653	the rapid and conformal deposition	620:653	the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability	620:730	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	6	49	theme	multilayered	954:965	arg1	films					974:978	multilayered hybrid films	954:978	multilayered hybrid films	954:978	In addition, metal nanoparticles were embedded into the L-PG layers to build up multilayered hybrid films.					
32219272	7	50	theme	multilayered	1036:1047	arg1	films					1049:1053	gold and silver nanoparticle-loaded L-PG multilayered films	995:1053	films	1049:1053	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	7	51	theme	catalytic	1060:1068	arg1	properties					1108:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	catalytic and surface-enhanced Raman scattering properties	1060:1117	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	2	52	theme	multifunctional	312:326	arg1	films					335:339	multifunctional hybrid films	312:339	multifunctional hybrid films	312:339	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	6	53	theme	L-PG	930:933	arg1	layers					935:940	the L-PG layers	926:940	the L-PG layers	926:940	In addition, metal nanoparticles were embedded into the L-PG layers to build up multilayered hybrid films.					
32219272	2	54	theme	lipoate-conjugated	401:418	arg1	phytoglycogen					420:432	lipoate-conjugated phytoglycogen	401:432	lipoate-conjugated phytoglycogen (L-PG)	401:439	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	2	54	theme	lipoate-conjugated	401:418	arg1	L-PG					435:438	L-PG	435:438	L-PG	435:438	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	2	55	theme	hybrid	328:333	arg1	films					335:339	multifunctional hybrid films	312:339	multifunctional hybrid films	312:339	Herein, we report the engineering of multifunctional hybrid films by assembling adhesive biological nanoparticles composed of lipoate-conjugated phytoglycogen (L-PG).					
32219272	6	56	from	addition	877:884	arg1	embedded					912:919	embedded	912:919	embedded	912:919	In addition, metal nanoparticles were embedded into the L-PG layers to build up multilayered hybrid films.					
32219272	7	57	theme	nanoparticle-loaded	1011:1029	arg1	films					1049:1053	gold and silver nanoparticle-loaded L-PG multilayered films	995:1053	films	1049:1053	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	3	58	theme	disulfide	561:569	arg1	groups					571:576	reactive functional disulfide groups	541:576	reactive functional disulfide groups	541:576	These nano building blocks possess adhesive properties, arising from their amphiphilic nature, and reactive functional disulfide groups.					
32219272	7	59	theme	L-PG	1031:1034	arg1	films					1049:1053	gold and silver nanoparticle-loaded L-PG multilayered films	995:1053	films	1049:1053	Specifically, gold and silver nanoparticle-loaded L-PG multilayered films with catalytic and surface-enhanced Raman scattering properties were prepared.					
32219272	4	60	theme	chemical	695:702	arg1	composition					704:714	varying chemical composition	687:714	varying chemical composition	687:714	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
32219272	8	61	dep	catalysis	1236:1244	arg1	applications					1258:1269	applications	1258:1269	applications	1258:1269	Finally, we highlight the versatility of the present approach to engineer multifaceted interfaces for catalysis and sensing applications.					
32219272	0	62	theme	hybrid	32:37	arg1	interfaces					39:48	multifunctional hybrid interfaces	16:48	multifunctional hybrid interfaces using adhesive glycogen nanoparticles	16:86	Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles.					
32219272	4	63	theme	varying	687:693	arg1	composition					704:714	varying chemical composition	687:714	varying chemical composition	687:714	The assembly of L-PG on surfaces enabled the rapid and conformal deposition of a thin film on substrates of varying chemical composition and wettability.					
33831823	2	0	theme	ChGS	489:492	arg1	expressions					474:484	the expressions	470:484	the expressions of ChGS and ChGP	470:501	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	6	1	theme	lipid	920:924	arg1	content					926:932	lipid content	920:932	lipid content	920:932	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	7	2	dep	stage	1193:1197	arg1	August					1220:1225	August	1220:1225	August	1220:1225	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	1	3	theme	biochemical	157:167	arg1	composition					169:179	biochemical composition	157:179	biochemical composition	157:179	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	4	4	theme	significant	570:580	arg1	change					582:587	The only significant change	561:587	The only significant change in protein levels	561:605	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	4	4	theme	significant	570:580	arg1	increase					614:621	an increase	611:621	an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation	611:754	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	0	5	theme	hongkongensis	86:98	arg1	composition					35:45	biochemical composition	23:45	biochemical composition	23:45	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	0	5	theme	hongkongensis	86:98	arg1	quality					63:69	nutritional quality	51:69	nutritional quality	51:69	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	0	6	from	variations	9:18	arg1	composition					35:45	biochemical composition	23:45	biochemical composition	23:45	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	0	6	from	variations	9:18	arg1	quality					63:69	nutritional quality	51:69	nutritional quality	51:69	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	1	7	with	composition	169:179	arg1	changes					217:223	annual changes	210:223	annual changes in gonad development	210:244	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	3	8	theme	Protein	504:510	arg1	content					512:518	Protein content	504:518	Protein content	504:518	Protein content consistently exceeded 52% of dry weight.					
33831823	2	9	from	levels	338:343	arg1	mantle					352:357	mantle	352:357	mantle	352:357	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	2	9	from	levels	338:343	arg1	mass					387:390	gonad-visceral mass	372:390	gonad-visceral mass	372:390	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	2	9	from	levels	338:343	arg1	muscle					360:365	muscle	360:365	muscle	360:365	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	6	10	theme	reproductive	997:1008	arg1	cycle					1010:1014	the reproductive cycle	993:1014	the reproductive cycle	993:1014	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	4	11	from	proliferation	728:740	arg1	transition					712:721	the gonadal transition	700:721	the gonadal transition from proliferation to maturation	700:754	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	7	12	theme	inactive	1184:1191	arg1	stage					1193:1197	the inactive stage	1180:1197	the inactive stage of most gonads	1180:1212	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	3	13	theme	weight	553:558	arg1	%					544:544	52%	542:544	52% of dry weight	542:558	Protein content consistently exceeded 52% of dry weight.					
33831823	3	13	theme	weight	553:558	arg1	weight					553:558	dry weight	549:558	dry weight	549:558	Protein content consistently exceeded 52% of dry weight.					
33831823	1	14	theme	optimal	280:286	arg1	time					299:302	the optimal harvesting time	276:302	the optimal harvesting time of C. hongkongensis	276:322	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	4	15	from	change	582:587	arg1	levels					600:605	protein levels	592:605	protein levels	592:605	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	0	16	from	quality	63:69	arg1	relation					104:111	relation	104:111	relation to the gametogenic cycle	104:136	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	5	17	theme	preschool	896:904	arg1	children					906:913	preschool children	896:913	preschool children	896:913	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	1	18	theme	harvesting	288:297	arg1	time					299:302	the optimal harvesting time	276:302	the optimal harvesting time of C. hongkongensis	276:322	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	7	19	theme	hongkongensis	1155:1167	arg1	time					1144:1147	the optimal harvest time	1124:1147	the optimal harvest time of C. hongkongensis	1124:1167	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	2	20	from	changes	459:465	arg1	expressions					474:484	the expressions	470:484	the expressions of ChGS and ChGP	470:501	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	5	21	theme	amino	869:873	arg1	requirements					880:891	the essential amino acid requirements	855:891	the essential amino acid requirements of preschool children	855:913	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	5	22	theme	children	906:913	arg1	requirements					880:891	the essential amino acid requirements	855:891	the essential amino acid requirements of preschool children	855:913	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	5	23	theme	well-balanced	803:815	arg1	profile					838:844	a well-balanced essential amino acid profile	801:844	a well-balanced essential amino acid profile	801:844	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	1	24	theme	nutritional	185:195	arg1	quality					197:203	nutritional quality	185:203	nutritional quality	185:203	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	0	25	from	composition	35:45	arg1	relation					104:111	relation	104:111	relation to the gametogenic cycle	104:136	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	1	26	from	Variations	139:148	arg1	quality					197:203	nutritional quality	185:203	nutritional quality	185:203	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	1	26	from	Variations	139:148	arg1	composition					169:179	biochemical composition	157:179	biochemical composition	157:179	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	4	27	theme	gonad-visceral	653:666	arg1	mass					668:671	the gonad-visceral mass	649:671	the gonad-visceral mass	649:671	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	0	28	theme	Seasonal	0:7	arg1	variations					9:18	Seasonal variations	0:18	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.	0:137	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	1	29	dep	composition	169:179	arg1	the					153:155	the	153:155	the	153:155	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	5	30	theme	acid	875:878	arg1	requirements					880:891	the essential amino acid requirements	855:891	the essential amino acid requirements of preschool children	855:913	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	0	31	theme	biochemical	23:33	arg1	composition					35:45	biochemical composition	23:45	biochemical composition	23:45	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	6	32	theme	hongkongensis	967:979	arg1	composition					949:959	fatty acid composition	938:959	fatty acid composition	938:959	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	6	32	theme	hongkongensis	967:979	arg1	content					926:932	lipid content	920:932	lipid content	920:932	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	3	33	theme	dry	549:551	arg1	weight					553:558	dry weight	549:558	dry weight	549:558	Protein content consistently exceeded 52% of dry weight.					
33831823	1	34	theme	annual	210:215	arg1	changes					217:223	annual changes	210:223	annual changes in gonad development	210:244	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	2	35	theme	glycogen	329:336	arg1	lower					411:415	lower	411:415	lower	411:415	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	2	35	theme	glycogen	329:336	arg1	levels					338:343	The glycogen levels	325:343	The glycogen levels in the mantle, muscle, and gonad-visceral mass	325:390	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	0	36	theme	gametogenic	120:130	arg1	cycle					132:136	the gametogenic cycle	116:136	the gametogenic cycle	116:136	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	2	37	dep	mantle	352:357	arg1	the					348:350	the	348:350	the	348:350	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	5	38	theme	C.	767:768	arg1	hongkongensis					770:782	C. hongkongensis	767:782	C. hongkongensis	767:782	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	1	39	theme	hongkongensis	310:322	arg1	time					299:302	the optimal harvesting time	276:302	the optimal harvesting time of C. hongkongensis	276:322	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	6	40	theme	omega-6	1033:1039	arg1	ratio					1041:1045	the omega-3:omega-6 ratio	1021:1045	the omega-3:omega-6 ratio	1021:1045	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	6	40	theme	omega-6	1033:1039	arg1	higher					1064:1069	higher	1064:1069	higher	1064:1069	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	7	41	theme	optimal	1128:1134	arg1	time					1144:1147	the optimal harvest time	1124:1147	the optimal harvest time of C. hongkongensis	1124:1167	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	5	42	theme	essential	817:825	arg1	profile					838:844	a well-balanced essential amino acid profile	801:844	a well-balanced essential amino acid profile	801:844	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	1	43	theme	gonad	228:232	arg1	development					234:244	gonad development	228:244	gonad development	228:244	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	2	44	theme	ChGP	498:501	arg1	expressions					474:484	the expressions	470:484	the expressions of ChGS and ChGP	470:501	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	1	45	with	quality	197:203	arg1	changes					217:223	annual changes	210:223	annual changes in gonad development	210:244	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	7	46	theme	most	1202:1205	arg1	gonads					1207:1212	most gonads	1202:1212	most gonads	1202:1212	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	0	47	theme	nutritional	51:61	arg1	quality					63:69	nutritional quality	51:69	nutritional quality	51:69	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	6	48	theme	fatty	938:942	arg1	composition					949:959	fatty acid composition	938:959	fatty acid composition	938:959	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	5	49	contain	had	797:799	arg1	hongkongensis					770:782	C. hongkongensis	767:782	C. hongkongensis	767:782	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	5	49	contain	had	797:799	arg2	profile					838:844	a well-balanced essential amino acid profile	801:844	a well-balanced essential amino acid profile	801:844	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	7	50	theme	harvest	1136:1142	arg1	time					1144:1147	the optimal harvest time	1124:1147	the optimal harvest time of C. hongkongensis	1124:1167	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	7	51	from	stage	1193:1197	arg1	summary					1115:1121	summary	1115:1121	summary	1115:1121	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	1	52	from	changes	217:223	arg1	development					234:244	gonad development	228:244	gonad development	228:244	Variations in the biochemical composition and nutritional quality with annual changes in gonad development were investigated to identify the optimal harvesting time of C. hongkongensis.					
33831823	4	53	theme	gonadal	704:710	arg1	transition					712:721	the gonadal transition	700:721	the gonadal transition from proliferation to maturation	700:754	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	6	54	theme	omega-3	1025:1031	arg1	ratio					1041:1045	the omega-3:omega-6 ratio	1021:1045	the omega-3:omega-6 ratio	1021:1045	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	6	54	theme	omega-3	1025:1031	arg1	higher					1064:1069	higher	1064:1069	higher	1064:1069	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	4	55	theme	protein	592:598	arg1	levels					600:605	protein levels	592:605	protein levels	592:605	The only significant change in protein levels was an increase between April and June in the gonad-visceral mass, which was associated with the gonadal transition from proliferation to maturation.					
33831823	0	56	theme	Crassostrea	74:84	arg1	hongkongensis					86:98	Crassostrea hongkongensis	74:98	Crassostrea hongkongensis	74:98	Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle.					
33831823	7	57	dep	August	1220:1225	arg1	February					1230:1237	February	1230:1237	February	1230:1237	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	7	57	dep	August	1220:1225	arg1	to					1227:1228	to	1227:1228	to	1227:1228	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	5	58	theme	acid	833:836	arg1	profile					838:844	a well-balanced essential amino acid profile	801:844	a well-balanced essential amino acid profile	801:844	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	2	59	theme	gonad-visceral	372:385	arg1	mass					387:390	gonad-visceral mass	372:390	gonad-visceral mass	372:390	The glycogen levels in the mantle, muscle, and gonad-visceral mass were significantly lower in June than in December, associated with changes in the expressions of ChGS and ChGP.					
33831823	5	60	theme	essential	859:867	arg1	requirements					880:891	the essential amino acid requirements	855:891	the essential amino acid requirements of preschool children	855:913	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	6	61	dep	content	926:932	arg1	The					916:918	The	916:918	The	916:918	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
33831823	5	62	theme	amino	827:831	arg1	profile					838:844	a well-balanced essential amino acid profile	801:844	a well-balanced essential amino acid profile	801:844	Moreover, C. hongkongensis consistently had a well-balanced essential amino acid profile, meeting the essential amino acid requirements of preschool children.					
33831823	7	63	theme	gonads	1207:1212	arg1	stage					1193:1197	the inactive stage	1180:1197	the inactive stage of most gonads	1180:1212	In summary, the optimal harvest time of C. hongkongensis was during the inactive stage of most gonads (from August to February at Beihai).					
33831823	6	64	theme	acid	944:947	arg1	composition					949:959	fatty acid composition	938:959	fatty acid composition	938:959	The lipid content and fatty acid composition of C. hongkongensis varied with the reproductive cycle, but the omega-3:omega-6 ratio was consistently higher than those of C. gigas and C. virginica.					
34327147	3	0	from	outcomes	452:459	arg1	mice					516:519	mice	516:519	mice after stroke	516:532	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	7	1	theme	Results	915:921	arg1	supplementation					933:947	Results Lactulose supplementation	915:947	Results Lactulose supplementation	915:947	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	0	2	theme	Inflammatory	87:98	arg1	Reactions					100:108	Inflammatory Reactions	87:108	Inflammatory Reactions in Mice After Stroke	87:129	Lactulose Improves Neurological Outcomes by Repressing Harmful Bacteria and Regulating Inflammatory Reactions in Mice After Stroke.					
34327147	6	3	theme	untargeted	843:852	arg1	metabolomics					854:865	plasma untargeted metabolomics	836:865	plasma untargeted metabolomics	836:865	16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.					
34327147	2	4	dep	potential	320:328	arg1	therapeutic					330:340	therapeutic	330:340	therapeutic	330:340	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	9	5	from	stroke	1331:1336	arg1	mice					1341:1344	mice	1341:1344	mice	1341:1344	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	6	6	theme	plasma	836:841	arg1	metabolomics					854:865	plasma untargeted metabolomics	836:865	plasma untargeted metabolomics	836:865	16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.					
34327147	7	7	dep	brain	1095:1099	arg1	the					1091:1093	the	1091:1093	the	1091:1093	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	9	8	theme	harmful	1387:1393	arg1	bacteria					1395:1402	harmful bacteria	1387:1402	harmful bacteria	1387:1402	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	9	9	theme	metabolic	1409:1417	arg1	disorder					1419:1426	metabolic disorder	1409:1426	metabolic disorder	1409:1426	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	7	10	theme	stroke	998:1003	arg1	outcome					987:993	the functional outcome	972:993	the functional outcome of stroke	972:1003	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	7	10	theme	stroke	998:1003	arg1	factors					1075:1081	increased anti-inflammatory factors	1047:1081	increased anti-inflammatory factors	1047:1081	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	7	10	theme	stroke	998:1003	arg1	reaction					1033:1040	downregulated inflammatory reaction	1006:1040	downregulated inflammatory reaction	1006:1040	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	2	11	theme	potential	320:328	arg1	Administration					246:259	Administration	246:259	Administration of prebiotics to counteract post-stroke dysbiosis	246:309	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	2	11	theme	potential	320:328	arg1	strategy					342:349	a potential therapeutic strategy	318:349	a potential therapeutic strategy to improve neurological function	318:382	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	4	12	theme	healthy	612:618	arg1	groups					604:609	three groups	598:609	three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose	598:715	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	12	theme	healthy	612:618	arg1	control					620:626	healthy control	612:626	healthy control	612:626	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	8	13	theme	metabolic	1242:1250	arg1	disorder					1252:1259	metabolic disorder	1242:1259	metabolic disorder after stroke	1242:1272	In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.					
34327147	4	14	theme	triple-distilled	654:669	arg1	groups					604:609	three groups	598:609	three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose	598:715	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	14	theme	triple-distilled	654:669	arg1	water					671:675	triple-distilled water	654:675	triple-distilled water	654:675	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	6	15	theme	rDNA	819:822	arg1	sequencing					824:833	16S rDNA sequencing	815:833	16S rDNA sequencing	815:833	16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.					
34327147	1	16	dep	Background	132:141	arg1	dysbiosis					172:180	Gut microbiota dysbiosis	157:180	Gut microbiota dysbiosis	157:180	Background and Objective Gut microbiota dysbiosis following stroke affects the recovery of neurological function.					
34327147	4	17	theme	photothrombotic	682:696	arg1	stroke					698:703	photothrombotic stroke	682:703	photothrombotic stroke	682:703	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	17	theme	photothrombotic	682:696	arg1	groups					604:609	three groups	598:609	three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose	598:715	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	6	18	theme	16S	815:817	arg1	sequencing					824:833	16S rDNA sequencing	815:833	16S rDNA sequencing	815:833	16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.					
34327147	2	19	theme	neurological	362:373	arg1	function					375:382	neurological function	362:382	neurological function	362:382	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	3	20	theme	microbiota	466:475	arg1	composition					477:487	gut microbiota composition	462:487	gut microbiota composition	462:487	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	3	21	theme	neurological	430:441	arg1	outcomes					452:459	neurological function outcomes	430:459	neurological function outcomes	430:459	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	4	22	dep	Methods	535:541	arg1	mice					556:559	Male C57BL/6 mice	543:559	Methods Male C57BL/6 mice (20-25 g)	535:569	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	22	dep	Methods	535:541	arg1	g					568:568	20-25 g	562:568	20-25 g	562:568	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	9	23	theme	functional	1305:1314	arg1	outcomes					1316:1323	functional outcomes	1305:1323	functional outcomes	1305:1323	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	4	24	theme	Male	543:546	arg1	mice					556:559	Male C57BL/6 mice	543:559	Methods Male C57BL/6 mice (20-25 g)	535:569	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	24	theme	Male	543:546	arg1	g					568:568	20-25 g	562:568	20-25 g	562:568	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	7	25	theme	functional	976:985	arg1	outcome					987:993	the functional outcome	972:993	the functional outcome of stroke	972:1003	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	7	26	theme	downregulated	1006:1018	arg1	reaction					1033:1040	downregulated inflammatory reaction	1006:1040	downregulated inflammatory reaction	1006:1040	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	3	27	theme	lactulose	417:425	arg1	effect					407:412	the effect	403:412	the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke	403:532	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	8	28	theme	lactulose	1123:1131	arg1	supplementation					1133:1147	lactulose supplementation	1123:1147	lactulose supplementation	1123:1147	In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.					
34327147	0	29	from	Reactions	100:108	arg1	Mice					113:116	Mice	113:116	Mice After Stroke	113:129	Lactulose Improves Neurological Outcomes by Repressing Harmful Bacteria and Regulating Inflammatory Reactions in Mice After Stroke.					
34327147	1	30	theme	neurological	223:234	arg1	function					236:243	neurological function	223:243	neurological function	223:243	Background and Objective Gut microbiota dysbiosis following stroke affects the recovery of neurological function.					
34327147	5	31	theme	lactulose	747:755	arg1	administration					757:770	lactulose administration	747:770	lactulose administration	747:770	After 14 consecutive days of lactulose administration, feces, plasma, and organs were collected.					
34327147	1	32	theme	function	236:243	arg1	recovery					211:218	the recovery	207:218	the recovery of neurological function	207:243	Background and Objective Gut microbiota dysbiosis following stroke affects the recovery of neurological function.					
34327147	3	33	theme	plasma	494:499	arg1	metabolites					501:511	plasma metabolites	494:511	plasma metabolites in mice after stroke	494:532	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	3	34	theme	gut	462:464	arg1	composition					477:487	gut microbiota composition	462:487	gut microbiota composition	462:487	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	9	35	theme	gut	1439:1441	arg1	disruption					1451:1460	gut barrier disruption	1439:1460	gut barrier disruption	1439:1460	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	5	36	theme	administration	757:770	arg1	days					739:742	14 consecutive days	724:742	14 consecutive days of lactulose administration	724:770	After 14 consecutive days of lactulose administration, feces, plasma, and organs were collected.					
34327147	2	37	theme	prebiotics	264:273	arg1	Administration					246:259	Administration	246:259	Administration of prebiotics to counteract post-stroke dysbiosis	246:309	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	2	37	theme	prebiotics	264:273	arg1	strategy					342:349	a potential therapeutic strategy	318:349	a potential therapeutic strategy to improve neurological function	318:382	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	3	38	theme	function	443:450	arg1	outcomes					452:459	neurological function outcomes	430:459	neurological function outcomes	430:459	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	9	39	theme	barrier	1443:1449	arg1	disruption					1451:1460	gut barrier disruption	1439:1460	gut barrier disruption	1439:1460	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	3	40	from	effect	407:412	arg1	metabolites					501:511	plasma metabolites	494:511	plasma metabolites in mice after stroke	494:532	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	3	40	from	effect	407:412	arg1	composition					477:487	gut microbiota composition	462:487	gut microbiota composition	462:487	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	3	40	from	effect	407:412	arg1	outcomes					452:459	neurological function outcomes	430:459	neurological function outcomes	430:459	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	3	41	from	metabolites	501:511	arg1	mice					516:519	mice	516:519	mice after stroke	516:532	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	0	42	theme	Harmful	55:61	arg1	Bacteria					63:70	Harmful Bacteria	55:70	Harmful Bacteria	55:70	Lactulose Improves Neurological Outcomes by Repressing Harmful Bacteria and Regulating Inflammatory Reactions in Mice After Stroke.					
34327147	8	43	theme	barrier	1169:1175	arg1	injury					1177:1182	intestinal barrier injury	1158:1182	intestinal barrier injury	1158:1182	In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.					
34327147	7	44	theme	anti-inflammatory	1057:1073	arg1	factors					1075:1081	increased anti-inflammatory factors	1047:1081	increased anti-inflammatory factors	1047:1081	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	4	45	dep	groups	604:609	arg1	stroke					698:703	photothrombotic stroke	682:703	photothrombotic stroke	682:703	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	45	dep	groups	604:609	arg1	groups					604:609	three groups	598:609	three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose	598:715	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	45	dep	groups	604:609	arg1	control					620:626	healthy control	612:626	healthy control	612:626	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	45	dep	groups	604:609	arg1	stroke					645:650	photothrombotic stroke	629:650	photothrombotic stroke	629:650	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	45	dep	groups	604:609	arg1	lactulose					707:715	lactulose	707:715	lactulose	707:715	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	7	46	theme	increased	1047:1055	arg1	factors					1075:1081	increased anti-inflammatory factors	1047:1081	increased anti-inflammatory factors	1047:1081	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	8	47	theme	intestinal	1158:1167	arg1	injury					1177:1182	intestinal barrier injury	1158:1182	intestinal barrier injury	1158:1182	In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.					
34327147	9	48	theme	Conclusion	1275:1284	arg1	Lactulose					1286:1294	Conclusion Lactulose	1275:1294	Conclusion Lactulose	1275:1294	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	7	49	theme	inflammatory	1020:1031	arg1	reaction					1033:1040	downregulated inflammatory reaction	1006:1040	downregulated inflammatory reaction	1006:1040	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	4	50	theme	photothrombotic	629:643	arg1	groups					604:609	three groups	598:609	three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose	598:715	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	50	theme	photothrombotic	629:643	arg1	stroke					645:650	photothrombotic stroke	629:650	photothrombotic stroke	629:650	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	5	51	theme	consecutive	727:737	arg1	days					739:742	14 consecutive days	724:742	14 consecutive days of lactulose administration	724:770	After 14 consecutive days of lactulose administration, feces, plasma, and organs were collected.					
34327147	7	52	theme	Lactulose	923:931	arg1	supplementation					933:947	Results Lactulose supplementation	915:947	Results Lactulose supplementation	915:947	Results Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut.					
34327147	8	53	theme	microbiota	1198:1207	arg1	dysbiosis					1209:1217	gut microbiota dysbiosis	1194:1217	gut microbiota dysbiosis	1194:1217	In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.					
34327147	2	54	theme	post-stroke	289:299	arg1	dysbiosis					301:309	post-stroke dysbiosis	289:309	post-stroke dysbiosis	289:309	Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function.					
34327147	3	55	from	composition	477:487	arg1	mice					516:519	mice	516:519	mice after stroke	516:532	We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.					
34327147	1	56	theme	Gut	157:159	arg1	dysbiosis					172:180	Gut microbiota dysbiosis	157:180	Gut microbiota dysbiosis	157:180	Background and Objective Gut microbiota dysbiosis following stroke affects the recovery of neurological function.					
34327147	9	57	theme	inflammatory	1476:1487	arg1	reactions					1489:1497	inflammatory reactions	1476:1497	inflammatory reactions	1476:1497	Conclusion Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.					
34327147	8	58	theme	gut	1194:1196	arg1	dysbiosis					1209:1217	gut microbiota dysbiosis	1194:1217	gut microbiota dysbiosis	1194:1217	In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.					
34327147	4	59	theme	C57BL/6	548:554	arg1	mice					556:559	Male C57BL/6 mice	543:559	Methods Male C57BL/6 mice (20-25 g)	535:569	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	4	59	theme	C57BL/6	548:554	arg1	g					568:568	20-25 g	562:568	20-25 g	562:568	Methods Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose.					
34327147	0	60	theme	Neurological	19:30	arg1	Outcomes					32:39	Neurological Outcomes	19:39	Neurological Outcomes	19:39	Lactulose Improves Neurological Outcomes by Repressing Harmful Bacteria and Regulating Inflammatory Reactions in Mice After Stroke.					
34327147	1	61	theme	microbiota	161:170	arg1	dysbiosis					172:180	Gut microbiota dysbiosis	157:180	Gut microbiota dysbiosis	157:180	Background and Objective Gut microbiota dysbiosis following stroke affects the recovery of neurological function.					
34327147	6	62	theme	flow	874:877	arg1	cytometry					879:887	flow cytometry	874:887	flow cytometry	874:887	16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.					
34370873	9	0	theme	arthroscopy	1863:1873	arg1	platform					1939:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform	1857:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes	1857:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	7	1	theme	elastic	1634:1640	arg1	modulus					1642:1648	elastic modulus	1634:1648	elastic modulus of ovine explants (p < 0.001)	1634:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	3	2	theme	Technological	471:483	arg1	innovations					500:510	Technological and analytical innovations	471:510	innovations	500:510	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	9	3	theme	Raman	1857:1861	arg1	platform					1939:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform	1857:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes	1857:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	7	4	theme	variation	1621:1629	arg1	%					1612:1612	94%	1610:1612	94% of the variation in elastic modulus of ovine explants (p < 0.001)	1610:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	7	4	theme	variation	1621:1629	arg1	variation					1621:1629	the variation	1617:1629	the variation in elastic modulus of ovine explants (p < 0.001)	1617:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	4	5	theme	GAG-depleted	996:1007	arg1	explants					1026:1033	chemically GAG-depleted bovine cartilage explants	985:1033	(1) chemically GAG-depleted bovine cartilage explants (n = 40)	981:1042	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	9	6	theme	outcomes	1986:1993	arg1	monitoring					1962:1971	objective monitoring	1952:1971	objective monitoring of treatment outcomes	1952:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	5	7	theme	variation	1290:1298	arg1	p < 0.001					1398:1406	p < 0.001	1398:1406	p < 0.001	1398:1406	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	7	theme	variation	1290:1298	arg1	%					1281:1281	96%	1279:1281	96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1279:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	7	theme	variation	1290:1298	arg1	variation					1290:1298	the variation	1286:1298	the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1286:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	7	theme	variation	1290:1298	arg1	%					1267:1267	95%	1265:1267	95%	1265:1267	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	7	theme	variation	1290:1298	arg1	%					1272:1272	66%	1270:1272	66%	1270:1272	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	3	8	theme	Raman	524:528	arg1	analysis					530:537	Raman analysis	524:537	Raman analysis	524:537	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	7	9	theme	linear	1528:1533	arg1	regression					1535:1544	The multivariate linear regression	1511:1544	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores)	1511:1598	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	4	10	dep	explants	1087:1094	arg1	2					1046:1046	2	1046:1046	2	1046:1046	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	11	theme	abraded	1062:1068	arg1	n = 30					1097:1102	n = 30	1097:1102	n = 30	1097:1102	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	11	theme	abraded	1062:1068	arg1	explants					1087:1094	(2) mechanically abraded bovine cartilage explants	1045:1094	(2) mechanically abraded bovine cartilage explants (n = 30)	1045:1103	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	1	12	theme	biomarkers	166:175	arg1	lack					145:148	the lack	141:148	the lack of standardized biomarkers of cartilage health that can be applied in clinical trials	141:234	The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials.					
34370873	7	13	dep	biomarkers	1567:1576	arg1	scores					1592:1597	H2 O scores	1587:1597	H2 O scores	1587:1597	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	7	13	dep	biomarkers	1567:1576	arg1	GAG					1579:1581	GAG	1579:1581	GAG	1579:1581	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	8	14	theme	in	1716:1717	arg1	assessment					1742:1751	the first in vivo Raman arthroscopy assessment	1706:1751	the first in vivo Raman arthroscopy assessment of an ovine femoral condyle	1706:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	7	15	link	Raman-derived	1549:1561	arg1	biomarkers					1567:1576	Raman-derived ECM biomarkers	1549:1576	Raman-derived ECM biomarkers (GAG and H2 O scores)	1549:1598	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	2	16	theme	Raman	269:273	arg1	probe					275:279	a novel arthroscopic Raman probe	248:279	a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease	248:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	1	17	theme	cartilage	180:188	arg1	health					190:195	cartilage health	180:195	cartilage health that can be applied in clinical trials	180:234	The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials.					
34370873	4	18	dep	explants	1202:1209	arg1	4					1156:1156	4	1156:1156	4	1156:1156	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	3	19	theme	multiplexed	718:728	arg1	spectroscopy					746:757	multiplexed polarized Raman spectroscopy	718:757	multiplexed polarized Raman spectroscopy	718:757	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	8	20	theme	Raman	1724:1728	arg1	assessment					1742:1751	the first in vivo Raman arthroscopy assessment	1706:1751	the first in vivo Raman arthroscopy assessment of an ovine femoral condyle	1706:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	3	21	theme	multivariate	551:562	arg1	decomposition					564:576	(1) multivariate decomposition	547:576	(1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers	547:644	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	7	22	theme	H2	1587:1588	arg1	scores					1592:1597	H2 O scores	1587:1597	H2 O scores	1587:1597	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	9	23	theme	toward a	1875:1882	arg1	platform					1939:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform	1857:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes	1857:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	4	24	dep	performed	930:938	arg1	explants					1202:1209	(4) anatomical-site-varied ovine osteochondral explants	1155:1209	(4) anatomical-site-varied ovine osteochondral explants (n = 6)	1155:1217	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	24	dep	performed	930:938	arg1	explants					1132:1139	(3) aging human cartilage explants	1106:1139	(3) aging human cartilage explants (n = 14)	1106:1148	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	24	dep	performed	930:938	arg1	n = 6					1212:1216	n = 6	1212:1216	n = 6	1212:1216	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	24	dep	performed	930:938	arg1	n = 14					1142:1147	n = 14	1142:1147	n = 14	1142:1147	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	24	dep	performed	930:938	arg1	explants					1087:1094	(2) mechanically abraded bovine cartilage explants	1045:1094	(2) mechanically abraded bovine cartilage explants (n = 30)	1045:1103	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	24	dep	performed	930:938	arg1	explants					1026:1033	chemically GAG-depleted bovine cartilage explants	985:1033	(1) chemically GAG-depleted bovine cartilage explants (n = 40)	981:1042	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	24	dep	performed	930:938	arg1	n = 30					1097:1102	n = 30	1097:1102	n = 30	1097:1102	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	6	25	theme	RCAF	1410:1413	arg1	values					1415:1420	RCAF values	1410:1420	RCAF values	1410:1420	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	4	26	theme	cartilage	1077:1085	arg1	n = 30					1097:1102	n = 30	1097:1102	n = 30	1097:1102	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	26	theme	cartilage	1077:1085	arg1	explants					1087:1094	(2) mechanically abraded bovine cartilage explants	1045:1094	(2) mechanically abraded bovine cartilage explants (n = 30)	1045:1103	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	3	27	theme	Raman	740:744	arg1	spectroscopy					746:757	multiplexed polarized Raman spectroscopy	718:757	multiplexed polarized Raman spectroscopy	718:757	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	8	28	theme	femoral	1765:1771	arg1	condyle					1773:1779	an ovine femoral condyle	1756:1779	an ovine femoral condyle	1756:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	3	29	theme	Raman	591:595	arg1	spectra					597:603	cartilage Raman spectra	581:603	cartilage Raman spectra	581:603	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	7	30	theme	biomarkers	1567:1576	arg1	regression					1535:1544	The multivariate linear regression	1511:1544	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores)	1511:1598	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	9	31	dep	low-cost	1899:1906	arg1	invasive					1919:1926	invasive	1919:1926	invasive	1919:1926	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	5	32	theme	bovine	1331:1336	arg1	explants					1338:1345	GAG-depleted bovine explants	1318:1345	GAG-depleted bovine explants	1318:1345	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	7	33	theme	Raman-derived	1549:1561	arg1	biomarkers					1567:1576	Raman-derived ECM biomarkers	1549:1576	Raman-derived ECM biomarkers (GAG and H2 O scores)	1549:1598	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	9	34	theme	transformative	1884:1897	arg1	platform					1939:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform	1857:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes	1857:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	4	35	theme	Raman	906:910	arg1	measurements					912:923	Raman measurements	906:923	Raman measurements	906:923	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	3	36	theme	superficial	771:781	arg1	anisotropy					797:806	superficial zone (SZ) COL anisotropy	771:806	superficial zone (SZ) COL anisotropy	771:806	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	2	37	theme	novel	250:254	arg1	probe					275:279	a novel arthroscopic Raman probe	248:279	a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease	248:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	6	38	theme	COL	1489:1491	arg1	p < 0.001					1499:1507	p < 0.001	1499:1507	p < 0.001	1499:1507	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	6	38	theme	COL	1489:1491	arg1	loss					1493:1496	abrasion-induced SZ COL loss	1469:1496	abrasion-induced SZ COL loss (p < 0.001)	1469:1508	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	3	39	theme	collagen	872:879	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	5	40	theme	GAG	1303:1305	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	41	theme	Derived	1220:1226	arg1	biomarkers					1244:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	6	42	theme	abrasion-induced	1469:1484	arg1	p < 0.001					1499:1507	p < 0.001	1499:1507	p < 0.001	1499:1507	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	6	42	theme	abrasion-induced	1469:1484	arg1	loss					1493:1496	abrasion-induced SZ COL loss	1469:1496	abrasion-induced SZ COL loss (p < 0.001)	1469:1508	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	3	43	theme	analysis-derived	849:864	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	8	44	theme	condyle	1773:1779	arg1	assessment					1742:1751	the first in vivo Raman arthroscopy assessment	1706:1751	the first in vivo Raman arthroscopy assessment of an ovine femoral condyle	1706:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	5	45	theme	GAG	1234:1236	arg1	biomarkers					1244:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	2	46	theme	matrix	350:355	arg1	biomarkers					363:372	key extracellular matrix (ECM) biomarkers	332:372	key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease	332:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	0	47	theme	Raman	0:4	arg1	arthroscopy					13:23	Raman needle arthroscopy	0:23	Raman needle arthroscopy for in vivo molecular assessment of cartilage.	0:70	Raman needle arthroscopy for in vivo molecular assessment of cartilage.					
34370873	4	48	dep	explants	1026:1033	arg1	1					982:982	1	982:982	1	982:982	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	5	49	theme	explants	1374:1381	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	4	50	theme	human	1116:1120	arg1	n = 14					1142:1147	n = 14	1142:1147	n = 14	1142:1147	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	50	theme	human	1116:1120	arg1	explants					1132:1139	(3) aging human cartilage explants	1106:1139	(3) aging human cartilage explants (n = 14)	1106:1148	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	3	51	theme	factor	891:896	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	5	52	from	%	1272:1272	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	4	53	theme	ex	955:956	arg1	models					973:978	ex vivo cartilage models	955:978	ex vivo cartilage models	955:978	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	9	54	theme	low-cost	1899:1906	arg1	platform					1939:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform	1857:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes	1857:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	0	55	theme	in	29:30	arg1	assessment					47:56	in vivo molecular assessment	29:56	in vivo molecular assessment of cartilage	29:69	Raman needle arthroscopy for in vivo molecular assessment of cartilage.					
34370873	4	56	theme	cartilage	963:971	arg1	models					973:978	ex vivo cartilage models	955:978	ex vivo cartilage models	955:978	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	5	57	theme	explants	1354:1361	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	4	58	theme	ovine	1182:1186	arg1	n = 6					1212:1216	n = 6	1212:1216	n = 6	1212:1216	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	58	theme	ovine	1182:1186	arg1	explants					1202:1209	(4) anatomical-site-varied ovine osteochondral explants	1155:1209	(4) anatomical-site-varied ovine osteochondral explants (n = 6)	1155:1217	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	0	59	theme	molecular	37:45	arg1	assessment					47:56	in vivo molecular assessment	29:56	in vivo molecular assessment of cartilage	29:69	Raman needle arthroscopy for in vivo molecular assessment of cartilage.					
34370873	0	60	dep	in	29:30	arg1	vivo					32:35	vivo	32:35	vivo	32:35	Raman needle arthroscopy for in vivo molecular assessment of cartilage.					
34370873	7	61	theme	ovine	1653:1657	arg1	explants					1659:1666	ovine explants	1653:1666	ovine explants (p < 0.001)	1653:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	7	61	theme	ovine	1653:1657	arg1	p < 0.001					1669:1677	p < 0.001	1669:1677	p < 0.001	1669:1677	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	4	62	theme	osteochondral	1188:1200	arg1	n = 6					1212:1216	n = 6	1212:1216	n = 6	1212:1216	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	62	theme	osteochondral	1188:1200	arg1	explants					1202:1209	(4) anatomical-site-varied ovine osteochondral explants	1155:1209	(4) anatomical-site-varied ovine osteochondral explants (n = 6)	1155:1217	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	9	63	theme	diagnostic	1928:1937	arg1	platform					1939:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform	1857:1946	Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes	1857:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	2	64	theme	key	332:334	arg1	biomarkers					363:372	key extracellular matrix (ECM) biomarkers	332:372	key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease	332:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	1	65	theme	clinical	220:227	arg1	trials					229:234	clinical trials	220:234	clinical trials	220:234	The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials.					
34370873	7	66	from	variation	1621:1629	arg1	modulus					1642:1648	elastic modulus	1634:1648	elastic modulus of ovine explants (p < 0.001)	1634:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	4	67	theme	bovine	1009:1014	arg1	explants					1026:1033	chemically GAG-depleted bovine cartilage explants	985:1033	(1) chemically GAG-depleted bovine cartilage explants (n = 40)	981:1042	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	9	68	theme	treatment	1976:1984	arg1	outcomes					1986:1993	treatment outcomes	1976:1993	treatment outcomes	1976:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	4	69	dep	ex	955:956	arg1	vivo					958:961	vivo	958:961	vivo	958:961	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	2	70	theme	arthroscopic	256:267	arg1	probe					275:279	a novel arthroscopic Raman probe	248:279	a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease	248:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	4	71	dep	explants	1132:1139	arg1	3					1107:1107	3	1107:1107	3	1107:1107	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	3	72	dep	decomposition	564:576	arg1	1					548:548	1	548:548	1	548:548	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	4	73	theme	bovine	1070:1075	arg1	n = 30					1097:1102	n = 30	1097:1102	n = 30	1097:1102	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	73	theme	bovine	1070:1075	arg1	explants					1087:1094	(2) mechanically abraded bovine cartilage explants	1045:1094	(2) mechanically abraded bovine cartilage explants (n = 30)	1045:1103	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	9	74	theme	OA	2009:2010	arg1	therapies					2012:2020	OA therapies	2009:2020	OA therapies	2009:2020	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	8	75	theme	first	1710:1714	arg1	assessment					1742:1751	the first in vivo Raman arthroscopy assessment	1706:1751	the first in vivo Raman arthroscopy assessment of an ovine femoral condyle	1706:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	1	76	theme	standardized	153:164	arg1	biomarkers					166:175	standardized biomarkers	153:175	standardized biomarkers of cartilage health that can be applied in clinical trials	153:234	The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials.					
34370873	5	77	from	%	1281:1281	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	8	78	theme	arthroscopy	1730:1740	arg1	assessment					1742:1751	the first in vivo Raman arthroscopy assessment	1706:1751	the first in vivo Raman arthroscopy assessment of an ovine femoral condyle	1706:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	1	79	theme	health	190:195	arg1	biomarkers					166:175	standardized biomarkers	153:175	standardized biomarkers of cartilage health that can be applied in clinical trials	153:234	The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials.					
34370873	3	80	theme	polarized	730:738	arg1	spectroscopy					746:757	multiplexed polarized Raman spectroscopy	718:757	multiplexed polarized Raman spectroscopy	718:757	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	O					698:698	O	698:698	O	698:698	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	scores					701:706	scores	701:706	scores	701:706	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	[COL					681:684	[COL	681:684	[COL	681:684	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	glycosaminoglycan					647:663	glycosaminoglycan	647:663	glycosaminoglycan	647:663	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	water					688:692	water	688:692	water	688:692	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	[GAG					665:668	[GAG	665:668	[GAG	665:668	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	[H2					694:696	[H2	694:696	[H2	694:696	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	81	dep	quantify	762:769	arg1	collagen					672:679	collagen	672:679	collagen	672:679	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	82	dep	innovations	500:510	arg1	optimize					515:522	optimize	515:522	to optimize Raman analysis	512:537	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	8	83	theme	ovine	1759:1763	arg1	condyle					1773:1779	an ovine femoral condyle	1756:1779	an ovine femoral condyle	1756:1779	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	3	84	theme	spectra	597:603	arg1	decomposition					564:576	(1) multivariate decomposition	547:576	(1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers	547:644	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	7	85	theme	O	1590:1590	arg1	scores					1592:1597	H2 O scores	1587:1597	H2 O scores	1587:1597	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	0	86	theme	cartilage	61:69	arg1	assessment					47:56	in vivo molecular assessment	29:56	in vivo molecular assessment of cartilage	29:69	Raman needle arthroscopy for in vivo molecular assessment of cartilage.					
34370873	7	87	theme	ECM	1563:1565	arg1	biomarkers					1567:1576	Raman-derived ECM biomarkers	1549:1576	Raman-derived ECM biomarkers (GAG and H2 O scores)	1549:1598	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	3	88	theme	cartilage	581:589	arg1	spectra					597:603	cartilage Raman spectra	581:603	cartilage Raman spectra	581:603	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	89	theme	COL	793:795	arg1	anisotropy					797:806	superficial zone (SZ) COL anisotropy	771:806	superficial zone (SZ) COL anisotropy	771:806	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	2	90	from	composition	400:410	arg1	disease					462:468	disease	462:468	disease	462:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	2	90	from	composition	400:410	arg1	health					451:456	health	451:456	health	451:456	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	8	91	theme	intraarticular	1789:1802	arg1	entry					1804:1808	intraarticular entry	1789:1808	intraarticular entry into the synovial capsule	1789:1834	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	3	92	theme	squares-discriminant	828:847	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	2	93	from	structure	413:421	arg1	disease					462:468	disease	462:468	disease	462:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	2	93	from	structure	413:421	arg1	health					451:456	health	451:456	health	451:456	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	5	94	theme	explants	1338:1345	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	3	95	theme	SZ	789:790	arg1	anisotropy					797:806	superficial zone (SZ) COL anisotropy	771:806	superficial zone (SZ) COL anisotropy	771:806	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	96	theme	partial	814:820	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	3	97	link	analysis-derived	849:864	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	5	98	theme	human	1348:1352	arg1	explants					1354:1361	human explants	1348:1361	human explants	1348:1361	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	2	99	dep	cartilage	309:317	arg1	biopsy					301:306	biopsy	301:306	biopsy	301:306	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	2	99	dep	cartilage	309:317	arg1	"					307:307	"	307:307	"	307:307	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	3	100	theme	zone	783:786	arg1	anisotropy					797:806	superficial zone (SZ) COL anisotropy	771:806	superficial zone (SZ) COL anisotropy	771:806	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	8	101	theme	synovial	1819:1826	arg1	capsule					1828:1834	the synovial capsule	1815:1834	the synovial capsule	1815:1834	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	6	102	theme	SZ	1486:1487	arg1	p < 0.001					1499:1507	p < 0.001	1499:1507	p < 0.001	1499:1507	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	6	102	theme	SZ	1486:1487	arg1	loss					1493:1496	abrasion-induced SZ COL loss	1469:1496	abrasion-induced SZ COL loss (p < 0.001)	1469:1508	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	3	103	theme	alignment	881:889	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	2	104	from	properties	437:446	arg1	disease					462:468	disease	462:468	disease	462:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	2	104	from	properties	437:446	arg1	health					451:456	health	451:456	health	451:456	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	4	105	theme	aging	1110:1114	arg1	n = 14					1142:1147	n = 14	1142:1147	n = 14	1142:1147	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	105	theme	aging	1110:1114	arg1	explants					1132:1139	(3) aging human cartilage explants	1106:1139	(3) aging human cartilage explants (n = 14)	1106:1148	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	5	106	theme	Raman	1228:1232	arg1	biomarkers					1244:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	0	107	theme	needle	6:11	arg1	arthroscopy					13:23	Raman needle arthroscopy	0:23	Raman needle arthroscopy for in vivo molecular assessment of cartilage.	0:70	Raman needle arthroscopy for in vivo molecular assessment of cartilage.					
34370873	5	108	from	content	1307:1313	arg1	p < 0.001					1398:1406	p < 0.001	1398:1406	p < 0.001	1398:1406	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	108	from	content	1307:1313	arg1	%					1281:1281	96%	1279:1281	96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1279:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	108	from	content	1307:1313	arg1	variation					1290:1298	the variation	1286:1298	the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1286:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	108	from	content	1307:1313	arg1	%					1267:1267	95%	1265:1267	95%	1265:1267	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	108	from	content	1307:1313	arg1	%					1272:1272	66%	1270:1272	66%	1270:1272	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	3	109	theme	Raman	866:870	arg1	RCAF					899:902	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF)	812:903	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	7	110	from	modulus	1642:1648	arg1	%					1612:1612	94%	1610:1612	94% of the variation in elastic modulus of ovine explants (p < 0.001)	1610:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	7	110	from	modulus	1642:1648	arg1	variation					1621:1629	the variation	1617:1629	the variation in elastic modulus of ovine explants (p < 0.001)	1617:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	4	111	theme	models	973:978	arg1	series					945:950	a series	943:950	a series of ex vivo cartilage models	943:978	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	5	112	theme	GAG-depleted	1318:1329	arg1	explants					1338:1345	GAG-depleted bovine explants	1318:1345	GAG-depleted bovine explants	1318:1345	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	4	113	theme	cartilage	1122:1130	arg1	n = 14					1142:1147	n = 14	1142:1147	n = 14	1142:1147	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	113	theme	cartilage	1122:1130	arg1	explants					1132:1139	(3) aging human cartilage explants	1106:1139	(3) aging human cartilage explants (n = 14)	1106:1148	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	2	114	theme	extracellular	336:348	arg1	ECM					358:360	ECM	358:360	ECM	358:360	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	2	114	theme	extracellular	336:348	arg1	matrix					350:355	extracellular matrix	336:355	key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease	332:468	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	1	115	theme	treatments	91:100	arg1	development					76:86	The development	72:86	The development of treatments for osteoarthritis (OA)	72:124	The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials.					
34370873	2	116	theme	cartilage	390:398	arg1	composition					400:410	cartilage composition	390:410	cartilage composition	390:410	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	4	117	theme	anatomical-site-varied	1159:1180	arg1	n = 6					1212:1216	n = 6	1212:1216	n = 6	1212:1216	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	4	117	theme	anatomical-site-varied	1159:1180	arg1	explants					1202:1209	(4) anatomical-site-varied ovine osteochondral explants	1155:1209	(4) anatomical-site-varied ovine osteochondral explants (n = 6)	1155:1217	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	7	118	from	%	1612:1612	arg1	modulus					1642:1648	elastic modulus	1634:1648	elastic modulus of ovine explants (p < 0.001)	1634:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	6	119	with	explants	1455:1462	arg1	p < 0.001					1499:1507	p < 0.001	1499:1507	p < 0.001	1499:1507	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	6	119	with	explants	1455:1462	arg1	loss					1493:1496	abrasion-induced SZ COL loss	1469:1496	abrasion-induced SZ COL loss (p < 0.001)	1469:1508	RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001).					
34370873	5	120	from	%	1267:1267	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	2	121	theme	material	428:435	arg1	properties					437:446	material properties	428:446	material properties	428:446	We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease.					
34370873	3	122	theme	ECM-constituent-specific	610:633	arg1	biomarkers					635:644	ECM-constituent-specific biomarkers	610:644	ECM-constituent-specific biomarkers	610:644	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34370873	8	123	dep	in	1716:1717	arg1	vivo					1719:1722	vivo	1719:1722	vivo	1719:1722	Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule.					
34370873	7	124	theme	explants	1659:1666	arg1	modulus					1642:1648	elastic modulus	1634:1648	elastic modulus of ovine explants (p < 0.001)	1634:1678	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	4	125	theme	cartilage	1016:1024	arg1	explants					1026:1033	chemically GAG-depleted bovine cartilage explants	985:1033	(1) chemically GAG-depleted bovine cartilage explants (n = 40)	981:1042	Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6).					
34370873	5	126	theme	ovine	1368:1372	arg1	explants					1374:1381	ovine explants	1368:1381	ovine explants	1368:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	5	127	theme	score	1238:1242	arg1	biomarkers					1244:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers	1220:1253	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	7	128	theme	multivariate	1515:1526	arg1	regression					1535:1544	The multivariate linear regression	1511:1544	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores)	1511:1598	The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001).					
34370873	5	129	from	variation	1290:1298	arg1	content					1307:1313	GAG content	1303:1313	GAG content of GAG-depleted bovine explants, human explants, and ovine explants	1303:1381	Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001).					
34370873	9	130	theme	objective	1952:1960	arg1	monitoring					1962:1971	objective monitoring	1952:1971	objective monitoring of treatment outcomes	1952:1993	This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.					
34370873	3	131	theme	analytical	489:498	arg1	innovations					500:510	Technological and analytical innovations	471:510	innovations	500:510	Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF).					
34178721	15	0	from	addition	1900:1907	arg1	formulations					1931:1942	the hydrogel formulations	1918:1942	the hydrogel formulations	1918:1942	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	1	1	theme	infections	255:264	arg1	insurgence					241:250	the insurgence	237:250	the insurgence of infections at the implantation site	237:289	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	4	2	theme	loaded	608:613	arg1	agents					629:634	the loaded antibacterial agents	604:634	the loaded antibacterial agents	604:634	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	13	3	theme	hydrogel	1782:1789	arg1	formulations					1791:1802	all the hydrogel formulations	1774:1802	all the hydrogel formulations	1774:1802	Results A homogeneous coating was obtained with all the hydrogel formulations.					
34178721	15	4	from	TA	1912:1913	arg1	formulations					1931:1942	the hydrogel formulations	1918:1942	the hydrogel formulations	1918:1942	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	16	5	dep	S.	2104:2105	arg1	aureus					2107:2112	aureus	2107:2112	aureus	2107:2112	Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels.					
34178721	9	6	theme	GS-loaded	1165:1173	arg1	formulations					1184:1195	The different GS-loaded hydrogel formulations	1151:1195	The different GS-loaded hydrogel formulations	1151:1195	The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation.					
34178721	18	7	theme	gentamicin-loaded	2396:2412	arg1	agarose-hydrogels					2414:2430	the hereby proposed gentamicin-loaded agarose-hydrogels	2376:2430	the hereby proposed gentamicin-loaded agarose-hydrogels	2376:2430	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	18	7	theme	gentamicin-loaded	2396:2412	arg1	Conclusions					2282:2292	Conclusions	2282:2292	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects	2282:2373	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	11	8	theme	ELISA	1566:1570	arg1	test					1572:1575	a specific ELISA test	1555:1575	a specific ELISA test	1555:1575	GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test.					
34178721	18	9	theme	surface	2496:2502	arg1	modification					2504:2515	the surface modification	2492:2515	the surface modification of spinal implants	2492:2534	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	6	10	theme	controlled	896:905	arg1	release					907:913	the controlled release	892:913	the controlled release of gentamicin (GS)	892:932	In this study, an agarose hydrogel-based coating has been developed for the controlled release of gentamicin (GS).					
34178721	15	11	theme	hydrogel	1922:1929	arg1	formulations					1931:1942	the hydrogel formulations	1918:1942	the hydrogel formulations	1918:1942	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	15	12	from	formulations	1931:1942	arg1	addition					1900:1907	The addition	1896:1907	The addition of TA in the hydrogel formulations	1896:1942	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	1	13	from	site	286:289	arg1	insurgence					241:250	the insurgence	237:250	the insurgence of infections at the implantation site	237:289	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	16	14	theme	GS-agarose	2137:2146	arg1	hydrogels					2148:2156	the GS-agarose hydrogels	2133:2156	the GS-agarose hydrogels	2133:2156	Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels.					
34178721	0	15	theme	Gentamicin	126:135	arg1	Release					115:121	the Controlled Release	100:121	the Controlled Release of Gentamicin	100:135	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	9	16	theme	Ti6Al4V-DOPA	1215:1226	arg1	surfaces					1228:1235	Ti6Al4V-DOPA surfaces	1215:1235	Ti6Al4V-DOPA surfaces	1215:1235	The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation.					
34178721	1	17	theme	disorders	204:212	arg1	treatment					186:194	the treatment	182:194	the treatment of back disorders	182:212	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	12	18	theme	coatings	1611:1618	arg1	cytotoxicity					1582:1593	The cytotoxicity	1578:1593	The cytotoxicity of the produced coatings against human cells	1578:1638	The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.					
34178721	18	19	theme	antibacterial	2353:2365	arg1	effects					2367:2373	high antibacterial effects	2348:2373	high antibacterial effects	2348:2373	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	14	20	theme	surface	1877:1883	arg1	roughness					1885:1893	micro-scale surface roughness	1865:1893	micro-scale surface roughness	1865:1893	Moreover, the coatings presented a hydrophilic behavior and micro-scale surface roughness.					
34178721	12	21	theme	aureus	1709:1714	arg1	bacteria					1716:1723	S. aureus bacteria	1706:1723	S. aureus bacteria	1706:1723	The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.					
34178721	5	22	theme	natural	713:719	arg1	Agarose					670:676	Agarose	670:676	Agarose	670:676	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	5	22	theme	natural	713:719	arg1	polysaccharide					721:734	a biocompatible natural polysaccharide	697:734	a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations	697:817	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	7	23	theme	DOPA	998:1001	arg1	solution					1004:1011	dopamine (DOPA) solution	988:1011	dopamine (DOPA) solution	988:1011	Methods Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution.					
34178721	18	24	mod	modification	2504:2515	arg1	implants					2527:2534	spinal implants	2520:2534	spinal implants	2520:2534	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	18	24	mod	modification	2504:2515	arg3	surface					2496:2502	the surface modification	2492:2515	the surface modification of spinal implants	2492:2534	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	9	25	theme	UV	1265:1266	arg1	irradiation					1268:1278	UV irradiation	1265:1278	UV irradiation	1265:1278	The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation.					
34178721	7	26	theme	dopamine	988:995	arg1	solution					1004:1011	dopamine (DOPA) solution	988:1011	dopamine (DOPA) solution	988:1011	Methods Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution.					
34178721	14	27	theme	hydrophilic	1840:1850	arg1	behavior					1852:1859	a hydrophilic behavior	1838:1859	a hydrophilic behavior	1838:1859	Moreover, the coatings presented a hydrophilic behavior and micro-scale surface roughness.					
34178721	12	28	theme	antibacterial	1675:1687	arg1	activity					1689:1696	their antibacterial activity	1669:1696	their antibacterial activity against S. aureus bacteria	1669:1723	The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.					
34178721	5	29	dep	polysaccharide	721:734	arg1	known					736:740	known	736:740	known to improve cell growth	736:763	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	5	29	dep	polysaccharide	721:734	arg1	used					777:780	used	777:780	already used in drug delivery system formulations	769:817	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	15	30	theme	release	1970:1976	arg1	time					1978:1981	the release time	1966:1981	the release time compared to the normal GS-agarose hydrogels	1966:2025	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	11	31	theme	released	1522:1529	arg1	GS					1531:1532	the released GS	1518:1532	the released GS	1518:1532	GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test.					
34178721	5	32	theme	delivery	790:797	arg1	formulations					806:817	drug delivery system formulations	785:817	drug delivery system formulations	785:817	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	1	33	theme	implants	169:176	arg1	use					155:157	The use	151:157	The use of spinal implants for the treatment of back disorders	151:212	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	4	34	theme	Hydrogel-based	515:528	arg1	systems					544:550	Hydrogel-based drug delivery systems	515:550	Hydrogel-based drug delivery systems	515:550	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	18	35	theme	spinal	2520:2525	arg1	implants					2527:2534	spinal implants	2520:2534	spinal implants	2520:2534	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	10	36	theme	angle	1382:1386	arg1	measurement					1388:1398	static contact angle measurement	1367:1398	static contact angle measurement	1367:1398	Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses.					
34178721	1	37	dep	Introduction	138:149	arg1	affected					225:232	affected	225:232	is largely affected by the insurgence of infections at the implantation site	214:289	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	8	38	theme	agarose	1031:1037	arg1	hydrogels					1039:1047	GS loaded agarose hydrogels	1021:1047	GS loaded agarose hydrogels	1021:1047	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	8	38	theme	agarose	1031:1037	arg1	crosslinkers					1137:1148	crosslinkers	1137:1148	crosslinkers	1137:1148	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	17	39	theme	TA-modified	2259:2269	arg1	hydrogels					2271:2279	the TA-modified hydrogels	2255:2279	the TA-modified hydrogels	2255:2279	The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.					
34178721	0	40	theme	Dopamine-Functionalized	25:47	arg1	Surfaces					58:65	Coat Dopamine-Functionalized Titanium Surfaces	20:65	Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels	20:94	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	7	41	theme	blasted	948:954	arg1	discs					964:968	Sand blasted Ti6Al4V discs	943:968	Methods Sand blasted Ti6Al4V discs	935:968	Methods Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution.					
34178721	1	42	theme	implantation	273:284	arg1	site					286:289	the implantation site	269:289	the implantation site	269:289	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	9	43	theme	hydrogel	1175:1182	arg1	formulations					1184:1195	The different GS-loaded hydrogel formulations	1151:1195	The different GS-loaded hydrogel formulations	1151:1195	The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation.					
34178721	11	44	theme	pseudo-physiological	1468:1487	arg1	conditions					1489:1498	pseudo-physiological conditions	1468:1498	pseudo-physiological conditions	1468:1498	GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test.					
34178721	15	45	theme	GS-agarose	2006:2015	arg1	hydrogels					2017:2025	the normal GS-agarose hydrogels	1995:2025	the normal GS-agarose hydrogels	1995:2025	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	0	46	theme	Coat	20:23	arg1	Surfaces					58:65	Coat Dopamine-Functionalized Titanium Surfaces	20:65	Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels	20:94	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	8	47	theme	tannic	1088:1093	arg1	TA					1101:1102	TA	1101:1102	TA	1101:1102	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	8	47	theme	tannic	1088:1093	arg1	acid					1095:1098	tannic acid	1088:1098	tannic acid (TA)	1088:1103	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	18	48	theme	surface	2307:2313	arg1	properties					2315:2324	their surface properties	2301:2324	their surface properties	2301:2324	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	4	49	theme	delivery	535:542	arg1	systems					544:550	Hydrogel-based drug delivery systems	515:550	Hydrogel-based drug delivery systems	515:550	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	0	50	theme	Titanium	49:56	arg1	Surfaces					58:65	Coat Dopamine-Functionalized Titanium Surfaces	20:65	Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels	20:94	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	18	51	from	application	2561:2571	arg1	scenario					2600:2607	the orthopedic surgical scenario	2576:2607	the orthopedic surgical scenario	2576:2607	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	10	52	theme	spectroscopy	1414:1425	arg1	analyses					1427:1434	FTIR spectroscopy analyses	1409:1434	FTIR spectroscopy analyses	1409:1434	Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses.					
34178721	18	53	theme	proposed	2387:2394	arg1	agarose-hydrogels					2414:2430	the hereby proposed gentamicin-loaded agarose-hydrogels	2376:2430	the hereby proposed gentamicin-loaded agarose-hydrogels	2376:2430	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	18	53	theme	proposed	2387:2394	arg1	Conclusions					2282:2292	Conclusions	2282:2292	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects	2282:2373	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	6	54	theme	hydrogel-based	846:859	arg1	coating					861:867	an agarose hydrogel-based coating	835:867	an agarose hydrogel-based coating	835:867	In this study, an agarose hydrogel-based coating has been developed for the controlled release of gentamicin (GS).					
34178721	4	55	theme	antibacterial	615:627	arg1	agents					629:634	the loaded antibacterial agents	604:634	the loaded antibacterial agents	604:634	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	8	56	theme	calcium	1109:1115	arg1	CaCl2					1127:1131	CaCl2	1127:1131	CaCl2	1127:1131	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	8	56	theme	calcium	1109:1115	arg1	chloride					1117:1124	calcium chloride	1109:1124	calcium chloride (CaCl2)	1109:1132	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	9	57	theme	different	1155:1163	arg1	formulations					1184:1195	The different GS-loaded hydrogel formulations	1151:1195	The different GS-loaded hydrogel formulations	1151:1195	The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation.					
34178721	2	58	theme	Antibacterial	292:304	arg1	solution					346:353	a viable solution	337:353	a viable solution to limit such infections	337:378	Antibacterial coatings have been proposed as a viable solution to limit such infections.					
34178721	2	58	theme	Antibacterial	292:304	arg1	coatings					306:313	Antibacterial coatings	292:313	Antibacterial coatings	292:313	Antibacterial coatings have been proposed as a viable solution to limit such infections.					
34178721	5	59	from	polysaccharide	721:734	arg1	particular					682:691	particular	682:691	particular	682:691	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	6	60	theme	gentamicin	918:927	arg1	release					907:913	the controlled release	892:913	the controlled release of gentamicin (GS)	892:932	In this study, an agarose hydrogel-based coating has been developed for the controlled release of gentamicin (GS).					
34178721	8	61	dep	loaded	1024:1029	arg1	GS					1021:1022	GS	1021:1022	GS	1021:1022	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	10	62	theme	contact	1374:1380	arg1	measurement					1388:1398	static contact angle measurement	1367:1398	static contact angle measurement	1367:1398	Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses.					
34178721	18	63	theme	orthopedic	2580:2589	arg1	scenario					2600:2607	the orthopedic surgical scenario	2576:2607	the orthopedic surgical scenario	2576:2607	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	18	64	theme	surgical	2591:2598	arg1	scenario					2600:2607	the orthopedic surgical scenario	2576:2607	the orthopedic surgical scenario	2576:2607	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	10	65	theme	FTIR	1409:1412	arg1	analyses					1427:1434	FTIR spectroscopy analyses	1409:1434	FTIR spectroscopy analyses	1409:1434	Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses.					
34178721	16	66	attach	released	2045:2052	arg2	able					2074:2077	able	2074:2077	able	2074:2077	Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels.					
34178721	16	66	attach	released	2045:2052	arg1	gels					2065:2068	these gels	2059:2068	these gels	2059:2068	Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels.					
34178721	16	66	attach	released	2045:2052	arg2	GS					2042:2043	the GS	2038:2043	the GS released from these gels	2038:2068	Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels.					
34178721	12	67	theme	produced	1602:1609	arg1	coatings					1611:1618	the produced coatings	1598:1618	the produced coatings	1598:1618	The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.					
34178721	15	68	theme	TA	1912:1913	arg1	addition					1900:1907	The addition	1896:1907	The addition of TA in the hydrogel formulations	1896:1942	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	11	69	theme	specific	1557:1564	arg1	test					1572:1575	a specific ELISA test	1555:1575	a specific ELISA test	1555:1575	GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test.					
34178721	18	70	theme	new	2440:2442	arg1	insight					2444:2450	new insight	2440:2450	new insight	2440:2450	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	4	71	theme	cell	652:655	arg1	integration					657:667	cell integration	652:667	cell integration	652:667	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	15	72	from	increase	1954:1961	arg1	time					1978:1981	the release time	1966:1981	the release time compared to the normal GS-agarose hydrogels	1966:2025	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	18	73	theme	high	2348:2351	arg1	effects					2367:2373	high antibacterial effects	2348:2373	high antibacterial effects	2348:2373	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	13	74	theme	homogeneous	1736:1746	arg1	coating					1748:1754	A homogeneous coating	1734:1754	A homogeneous coating	1734:1754	Results A homogeneous coating was obtained with all the hydrogel formulations.					
34178721	5	75	theme	cell	753:756	arg1	growth					758:763	cell growth	753:763	cell growth	753:763	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	5	76	theme	biocompatible	699:711	arg1	Agarose					670:676	Agarose	670:676	Agarose	670:676	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	5	76	theme	biocompatible	699:711	arg1	polysaccharide					721:734	a biocompatible natural polysaccharide	697:734	a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations	697:817	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	14	77	theme	micro-scale	1865:1875	arg1	roughness					1885:1893	micro-scale surface roughness	1865:1893	micro-scale surface roughness	1865:1893	Moreover, the coatings presented a hydrophilic behavior and micro-scale surface roughness.					
34178721	12	78	theme	S.	1706:1707	arg1	bacteria					1716:1723	S. aureus bacteria	1706:1723	S. aureus bacteria	1706:1723	The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.					
34178721	17	79	theme	CaCl2	2175:2179	arg1	able					2208:2211	able	2208:2211	able	2208:2211	The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.					
34178721	17	79	theme	CaCl2	2175:2179	arg1	addition					2163:2170	The addition	2159:2170	The addition of CaCl2 to the gel formulation	2159:2202	The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.					
34178721	0	80	theme	Agarose-Based	72:84	arg1	Hydrogels					86:94	Agarose-Based Hydrogels	72:94	Agarose-Based Hydrogels	72:94	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	7	81	dep	Methods	935:941	arg1	discs					964:968	Sand blasted Ti6Al4V discs	943:968	Methods Sand blasted Ti6Al4V discs	935:968	Methods Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution.					
34178721	5	82	theme	drug	785:788	arg1	formulations					806:817	drug delivery system formulations	785:817	drug delivery system formulations	785:817	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	8	83	theme	loaded	1024:1029	arg1	hydrogels					1039:1047	GS loaded agarose hydrogels	1021:1047	GS loaded agarose hydrogels	1021:1047	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	8	83	theme	loaded	1024:1029	arg1	crosslinkers					1137:1148	crosslinkers	1137:1148	crosslinkers	1137:1148	After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers.					
34178721	1	84	theme	spinal	162:167	arg1	implants					169:176	spinal implants	162:176	spinal implants	162:176	Introduction The use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site.					
34178721	4	85	theme	drug	530:533	arg1	systems					544:550	Hydrogel-based drug delivery systems	515:550	Hydrogel-based drug delivery systems	515:550	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	18	86	theme	implants	2527:2534	arg1	modification					2504:2515	the surface modification	2492:2515	the surface modification of spinal implants	2492:2534	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	0	87	theme	Controlled	104:113	arg1	Release					115:121	the Controlled Release	100:121	the Controlled Release of Gentamicin	100:135	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	3	88	from	short-term	417:426	arg1	effective					404:412	effective	404:412	effective	404:412	However, despite being effective at short-term, conventional coatings lack the ability to prevent infections at medium and long-term.					
34178721	0	89	with	Surfaces	58:65	arg1	Hydrogels					86:94	Agarose-Based Hydrogels	72:94	Agarose-Based Hydrogels	72:94	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	11	90	theme	GS	1437:1438	arg1	release					1440:1446	GS release	1437:1446	GS release	1437:1446	GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test.					
34178721	10	91	theme	Surface	1281:1287	arg1	topography					1289:1298	Surface topography	1281:1298	Surface topography	1281:1298	Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses.					
34178721	17	92	theme	hydrogels	2271:2279	arg1	cytotoxicity					2239:2250	cytotoxicity	2239:2250	cytotoxicity of the TA-modified hydrogels	2239:2279	The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.					
34178721	16	93	theme	S.	2104:2105	arg1	growth					2114:2119	S. aureus growth	2104:2119	S. aureus growth	2104:2119	Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels.					
34178721	11	94	dep	days	1509:1512	arg1	up					1500:1501	up	1500:1501	up	1500:1501	GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test.					
34178721	17	95	theme	gel	2188:2190	arg1	formulation					2192:2202	the gel formulation	2184:2202	the gel formulation	2184:2202	The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.					
34178721	0	96	theme	Novel	2:6	arg1	Strategy					8:15	A Novel Strategy	0:15	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.	0:136	A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin.					
34178721	13	97	dep	Results	1726:1732	arg1	obtained					1760:1767	obtained	1760:1767	was obtained with all the hydrogel formulations	1756:1802	Results A homogeneous coating was obtained with all the hydrogel formulations.					
34178721	7	98	theme	Ti6Al4V	956:962	arg1	discs					964:968	Sand blasted Ti6Al4V discs	943:968	Methods Sand blasted Ti6Al4V discs	935:968	Methods Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution.					
34178721	18	99	theme	Due	2294:2296	arg1	agarose-hydrogels					2414:2430	the hereby proposed gentamicin-loaded agarose-hydrogels	2376:2430	the hereby proposed gentamicin-loaded agarose-hydrogels	2376:2430	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	18	99	theme	Due	2294:2296	arg1	Conclusions					2282:2292	Conclusions	2282:2292	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects	2282:2373	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	3	100	theme	conventional	429:440	arg1	coatings					442:449	conventional coatings	429:449	conventional coatings	429:449	However, despite being effective at short-term, conventional coatings lack the ability to prevent infections at medium and long-term.					
34178721	7	101	theme	Sand	943:946	arg1	discs					964:968	Sand blasted Ti6Al4V discs	943:968	Methods Sand blasted Ti6Al4V discs	935:968	Methods Sand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution.					
34178721	12	102	theme	human	1628:1632	arg1	cells					1634:1638	human cells	1628:1638	human cells	1628:1638	The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.					
34178721	18	103	theme	low	2327:2329	arg1	cytotoxicity					2331:2342	low cytotoxicity	2327:2342	low cytotoxicity	2327:2342	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	18	104	theme	promising	2469:2477	arg1	approach					2479:2486	a promising approach	2467:2486	a promising approach for the surface modification of spinal implants	2467:2534	Conclusions Due to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.					
34178721	5	105	theme	system	799:804	arg1	formulations					806:817	drug delivery system formulations	785:817	drug delivery system formulations	785:817	Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations.					
34178721	15	106	theme	normal	1999:2004	arg1	hydrogels					2017:2025	the normal GS-agarose hydrogels	1995:2025	the normal GS-agarose hydrogels	1995:2025	The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels.					
34178721	2	107	theme	viable	339:344	arg1	solution					346:353	a viable solution	337:353	a viable solution to limit such infections	337:378	Antibacterial coatings have been proposed as a viable solution to limit such infections.					
34178721	2	107	theme	viable	339:344	arg1	coatings					306:313	Antibacterial coatings	292:313	Antibacterial coatings	292:313	Antibacterial coatings have been proposed as a viable solution to limit such infections.					
34178721	4	108	theme	agents	629:634	arg1	release					593:599	the release	589:599	the release of the loaded antibacterial agents	589:634	Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration.					
34178721	10	109	theme	static	1367:1372	arg1	measurement					1388:1398	static contact angle measurement	1367:1398	static contact angle measurement	1367:1398	Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses.					
34178721	6	110	theme	agarose	838:844	arg1	coating					861:867	an agarose hydrogel-based coating	835:867	an agarose hydrogel-based coating	835:867	In this study, an agarose hydrogel-based coating has been developed for the controlled release of gentamicin (GS).					
34178721	2	111	theme	such	364:367	arg1	infections					369:378	such infections	364:378	such infections	364:378	Antibacterial coatings have been proposed as a viable solution to limit such infections.					
34001358	14	0	from	level	2426:2430	arg1	milk					2439:2442	the milk	2435:2442	the milk	2435:2442	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	5	1	dep	ratios	977:982	arg1	milk					1005:1008	control milk	997:1008	control milk	997:1008	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	1	dep	ratios	977:982	arg1	1.8					992:994	1.8	992:994	1.8	992:994	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	1	dep	ratios	977:982	arg1	1.1					1017:1019	1.1	1017:1019	1.1	1017:1019	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	1	dep	ratios	977:982	arg1	0.9					1026:1028	0.9	1026:1028	0.9	1026:1028	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	1	dep	ratios	977:982	arg1	1.4					1012:1014	1.4	1012:1014	1.4	1012:1014	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	13	2	contain	had	2269:2271	arg2	lower					2273:2277	lower	2273:2277	lower	2273:2277	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	13	2	contain	had	2269:2271	arg1	contents					2260:2267	CN contents	2257:2267	CN contents	2257:2267	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	8	3	theme	whey	1559:1562	arg1	gel					1554:1556	the gel	1550:1556	the gel	1550:1556	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	3	theme	whey	1559:1562	arg1	drainage					1564:1571	whey drainage	1559:1571	whey drainage	1559:1571	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	14	4	theme	specific	2369:2376	arg1	ratio					2383:2387	a specific L:CN ratio	2367:2387	a specific L:CN ratio	2367:2387	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	12	5	theme	L	2101:2101	arg1	cheeses					2106:2112	all reduced L:CN cheeses	2089:2112	all reduced L:CN cheeses	2089:2112	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	3	6	theme	Cheddar	669:675	arg1	cheese					677:682	Cheddar cheese	669:682	Cheddar cheese	669:682	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	1	7	theme	buffering	260:268	arg1	capacity					270:277	the buffering capacity	256:277	the buffering capacity of the cheese	256:291	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	7	8	theme	ripening	1259:1266	arg1	mo					1253:1254	9 mo	1251:1254	9 mo of ripening	1251:1266	These milks were used to make milled-curd Cheddar cheese, and we evaluated cheese composition, texture, functionality, and sensory properties over 9 mo of ripening.					
34001358	5	9	with	milks	936:940	arg1	ratios					977:982	4 different lactose-to-casein ratios	947:982	4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9	947:1028	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	9	with	milks	936:940	arg1	L					985:985	L	985:985	L:CN	985:988	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	3	10	theme	acidity	658:664	arg1	development					633:643	the development	629:643	the development of excessive acidity in Cheddar cheese	629:682	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	12	11	theme	cheese	1995:2000	arg1	meltability					2002:2012	cheese meltability	1995:2012	cheese meltability	1995:2012	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	12	12	dep	L	2178:2178	arg1	CN					2180:2181	CN 1.8	2180:2185	L:CN 1.8	2178:2185	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	4	13	dep	ultrafiltration	841:855	arg1	permeate					857:864	permeate	857:864	permeate	857:864	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	8	14	dep	cutting	1542:1548	arg1	i.e.					1536:1539	i.e.	1536:1539	i.e.	1536:1539	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	10	15	contain	had	1788:1790	arg2	effect					1807:1812	no significant effect	1792:1812	no significant effect	1792:1812	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	10	15	contain	had	1788:1790	arg1	ratio					1782:1786	The L:CN ratio	1773:1786	The L:CN ratio	1773:1786	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	14	16	theme	constant	2410:2417	arg1	level					2426:2430	a constant casein level	2408:2430	a constant casein level in the milk	2408:2442	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	2	17	theme	buffering	298:306	arg1	capacity					308:315	The buffering capacity	294:315	The buffering capacity of cheese	294:325	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	4	18	theme	ultrafiltration	841:855	arg1	addition					822:829	the addition	818:829	the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content	818:920	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	10	19	theme	CN	1779:1780	arg1	ratio					1782:1786	The L:CN ratio	1773:1786	The L:CN ratio	1773:1786	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	3	20	from	cheese	677:682	arg1	development					633:643	the development	629:643	the development of excessive acidity in Cheddar cheese	629:682	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	3	21	theme	study	520:524	arg1	objective					502:510	The objective	498:510	The objective of this study	498:524	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	8	22	dep	steps	1529:1533	arg1	salting					1574:1580	salting	1574:1580	salting	1574:1580	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	22	dep	steps	1529:1533	arg1	gel					1554:1556	the gel	1550:1556	the gel	1550:1556	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	22	dep	steps	1529:1533	arg1	drainage					1564:1571	whey drainage	1559:1571	whey drainage	1559:1571	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	3	23	from	development	633:643	arg1	cheese					677:682	Cheddar cheese	669:682	Cheddar cheese	669:682	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	3	24	theme	lactose	554:560	arg1	contents					573:580	both the lactose and casein contents	545:580	contents	573:580	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	10	25	theme	significant	1795:1805	arg1	effect					1807:1812	no significant effect	1792:1812	no significant effect	1792:1812	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	14	26	theme	CN	2380:2381	arg1	ratio					2383:2387	a specific L:CN ratio	2367:2387	a specific L:CN ratio	2367:2387	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	5	27	theme	different	949:957	arg1	ratios					977:982	4 different lactose-to-casein ratios	947:982	4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9	947:1028	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	27	theme	different	949:957	arg1	L					985:985	L	985:985	L:CN	985:988	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	28	theme	lactose-to-casein	959:975	arg1	ratios					977:982	4 different lactose-to-casein ratios	947:982	4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9	947:1028	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	28	theme	lactose-to-casein	959:975	arg1	L					985:985	L	985:985	L:CN	985:988	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	12	29	dep	treatments	2061:2070	arg1	CN					2046:2047	CN	2046:2047	CN	2046:2047	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	13	30	theme	sulfury	2298:2304	arg1	notes					2306:2310	sulfury notes	2298:2310	sulfury notes	2298:2310	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	4	31	theme	water	834:838	arg1	addition					822:829	the addition	818:829	the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content	818:920	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	6	32	theme	casein	1065:1070	arg1	contents					1094:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	9	33	theme	lactic	1643:1648	arg1	acid					1650:1653	lower lactic acid	1637:1653	lower lactic acid	1637:1653	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	0	34	theme	Cheddar	170:176	arg1	cheese					178:183	milled-curd Cheddar cheese	158:183	milled-curd Cheddar cheese	158:183	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	8	35	theme	development	1505:1515	arg1	moisture					1333:1340	similar moisture	1325:1340	similar moisture	1325:1340	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	35	theme	development	1505:1515	arg1	control					1489:1495	control	1489:1495	similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development	1325:1515	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	35	theme	development	1505:1515	arg1	fat					1352:1354	fat	1352:1354	fat	1352:1354	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	35	theme	development	1505:1515	arg1	contents					1366:1373	salt contents	1361:1373	salt contents	1361:1373	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	35	theme	development	1505:1515	arg1	protein					1343:1349	protein	1343:1349	protein	1343:1349	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	12	36	theme	Maximum	1961:1967	arg1	tangent					1974:1980	Maximum loss tangent	1961:1980	Maximum loss tangent	1961:1980	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	12	36	theme	Maximum	1961:1967	arg1	index					1986:1990	an index	1983:1990	an index of cheese meltability	1983:2012	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	6	37	theme	similar	1051:1057	arg1	contents					1094:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	13	38	dep	cheeses	2218:2224	arg1	made					2226:2229	made	2226:2229	cheeses made from milks with reduced L	2218:2255	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	0	39	theme	factor	66:71	arg1	ultrafiltration					73:87	low-concentration factor ultrafiltration	48:87	low-concentration factor ultrafiltration	48:87	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	3	40	theme	casein	566:571	arg1	contents					573:580	both the lactose and casein contents	545:580	contents	573:580	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	8	41	theme	critical	1520:1527	arg1	steps					1529:1533	critical steps	1520:1533	critical steps (i.e., cutting the gel, whey drainage, salting)	1520:1581	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	14	42	theme	L	2378:2378	arg1	ratio					2383:2387	a specific L:CN ratio	2367:2387	a specific L:CN ratio	2367:2387	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	13	43	theme	Sensory	2189:2195	arg1	analyses					2197:2204	Sensory analyses	2189:2204	Sensory analyses	2189:2204	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	12	44	theme	control	2162:2168	arg1	cheese					2170:2175	the control cheese	2158:2175	the control cheese (L:CN 1.8)	2158:2186	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	2	45	theme	acidification	483:495	arg1	rate					475:478	the rate	471:478	the rate of acidification	471:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	8	46	theme	varying	1298:1304	arg1	levels					1306:1311	varying levels	1298:1311	varying levels of L	1298:1316	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	10	47	theme	secondary	1843:1851	arg1	proteolysis					1853:1863	primary and secondary proteolysis	1831:1863	primary and secondary proteolysis	1831:1863	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	9	48	with	cheeses	1624:1630	arg1	acid					1650:1653	lower lactic acid	1637:1653	lower lactic acid	1637:1653	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	9	48	with	cheeses	1624:1630	arg1	contents					1691:1698	insoluble Ca contents	1678:1698	insoluble Ca contents	1678:1698	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	9	48	with	cheeses	1624:1630	arg1	lactose					1665:1671	residual lactose	1656:1671	residual lactose	1656:1671	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	9	49	theme	Ca	1688:1689	arg1	contents					1691:1698	insoluble Ca contents	1678:1698	insoluble Ca contents	1678:1698	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	4	50	theme	low-concentration	719:735	arg1	ultrafiltration					744:758	low-concentration factor ultrafiltration	719:758	low-concentration factor ultrafiltration of milk	719:766	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	12	51	theme	meltability	2002:2012	arg1	tangent					1974:1980	Maximum loss tangent	1961:1980	Maximum loss tangent	1961:1980	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	12	51	theme	meltability	2002:2012	arg1	index					1986:1990	an index	1983:1990	an index of cheese meltability	1983:2012	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	7	52	theme	Cheddar	1146:1152	arg1	cheese					1154:1159	milled-curd Cheddar cheese	1134:1159	milled-curd Cheddar cheese	1134:1159	These milks were used to make milled-curd Cheddar cheese, and we evaluated cheese composition, texture, functionality, and sensory properties over 9 mo of ripening.					
34001358	2	53	theme	phosphate	428:436	arg1	milk					376:379	milk	376:379	milk	376:379	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	53	theme	phosphate	428:436	arg1	contents					364:371	the protein contents	352:371	the protein contents of milk and cheese	352:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	53	theme	phosphate	428:436	arg1	phosphate					428:436	insoluble calcium phosphate	410:436	insoluble calcium phosphate in the curd, which is related to the rate of acidification	410:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	53	theme	phosphate	428:436	arg1	cheese					385:390	cheese	385:390	cheese	385:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	53	theme	phosphate	428:436	arg1	amount					400:405	the amount	396:405	the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification	396:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	54	from	curd	445:448	arg1	milk					376:379	milk	376:379	milk	376:379	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	54	from	curd	445:448	arg1	contents					364:371	the protein contents	352:371	the protein contents of milk and cheese	352:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	54	from	curd	445:448	arg1	phosphate					428:436	insoluble calcium phosphate	410:436	insoluble calcium phosphate in the curd, which is related to the rate of acidification	410:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	54	from	curd	445:448	arg1	cheese					385:390	cheese	385:390	cheese	385:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	54	from	curd	445:448	arg1	amount					400:405	the amount	396:405	the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification	396:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	12	55	theme	maximum	2125:2131	arg1	values					2146:2151	higher maximum loss tangent values	2118:2151	higher maximum loss tangent values	2118:2151	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	11	56	theme	profile	1874:1880	arg1	analysis					1882:1889	Texture profile analysis	1866:1889	Texture profile analysis	1866:1889	Texture profile analysis showed no significant differences in hardness values during ripening.					
34001358	14	57	theme	tighter	2494:2500	arg1	control					2502:2508	tighter control	2494:2508	tighter control of pH and acidity	2494:2526	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	2	58	theme	insoluble	410:418	arg1	phosphate					428:436	insoluble calcium phosphate	410:436	insoluble calcium phosphate in the curd, which is related to the rate of acidification	410:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	14	59	theme	pH	2513:2514	arg1	control					2502:2508	tighter control	2494:2508	tighter control of pH and acidity	2494:2526	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	12	60	theme	tangent	2138:2144	arg1	values					2146:2151	higher maximum loss tangent values	2118:2151	higher maximum loss tangent values	2118:2151	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	7	61	used	used	1121:1124	arg2	milks					1110:1114	These milks	1104:1114	These milks	1104:1114	These milks were used to make milled-curd Cheddar cheese, and we evaluated cheese composition, texture, functionality, and sensory properties over 9 mo of ripening.					
34001358	0	62	theme	lactose	10:16	arg1	standardization					18:32	lactose standardization	10:32	lactose standardization of milk using low-concentration factor ultrafiltration	10:87	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	8	63	dep	Cheeses	1269:1275	arg1	had					1321:1323	had	1321:1323	had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting)	1321:1581	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	64	from	size	1459:1462	arg1	gel					1442:1444	cutting the gel	1430:1444	cutting the gel at a smaller size than control	1430:1475	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	14	65	theme	cheese	2465:2470	arg1	manufacturers					2472:2484	Cheddar cheese manufacturers	2457:2484	Cheddar cheese manufacturers	2457:2484	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	9	66	theme	pH	1726:1727	arg1	increase					1729:1736	a substantial pH increase	1712:1736	a substantial pH increase during cheese ripening in cheeses	1712:1770	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	4	67	theme	casein	784:789	arg1	content					791:797	the casein content	780:797	the casein content (∼5%)	780:803	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	4	67	theme	casein	784:789	arg1	%					802:802	∼5%	800:802	∼5%	800:802	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	13	68	theme	reduced	2247:2253	arg1	L					2255:2255	reduced L	2247:2255	reduced L	2247:2255	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	9	69	theme	substantial	1714:1724	arg1	increase					1729:1736	a substantial pH increase	1712:1736	a substantial pH increase during cheese ripening in cheeses	1712:1770	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	4	70	theme	ultrafiltration	744:758	arg1	use					712:714	the use	708:714	the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%)	708:803	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	2	71	theme	cheese	320:325	arg1	capacity					308:315	The buffering capacity	294:315	The buffering capacity of cheese	294:325	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	9	72	theme	residual	1656:1663	arg1	lactose					1665:1671	residual lactose	1656:1671	residual lactose	1656:1671	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	14	73	theme	milk	2359:2362	arg1	Standardization					2340:2354	Standardization	2340:2354	Standardization of milk to a specific L:CN ratio	2340:2387	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	12	74	theme	higher	2118:2123	arg1	values					2146:2151	higher maximum loss tangent values	2118:2151	higher maximum loss tangent values	2118:2151	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	11	75	theme	hardness	1928:1935	arg1	values					1937:1942	hardness values	1928:1942	hardness values	1928:1942	Texture profile analysis showed no significant differences in hardness values during ripening.					
34001358	4	76	theme	milk	763:766	arg1	ultrafiltration					744:758	low-concentration factor ultrafiltration	719:758	low-concentration factor ultrafiltration of milk	719:766	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	2	77	theme	protein	356:362	arg1	contents					364:371	the protein contents	352:371	the protein contents of milk and cheese	352:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	9	78	theme	L	1612:1612	arg1	CN					1614:1615	the L:CN	1608:1615	the L:CN	1608:1615	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	0	79	dep	Effect	0:5	arg1	Effect					90:95	Effect	90:95	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.	0:184	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	2	80	from	amount	400:405	arg1	related					460:466	related	460:466	related	460:466	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	80	from	amount	400:405	arg1	curd					445:448	the curd	441:448	the curd	441:448	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	12	81	theme	reduced	2093:2099	arg1	cheeses					2106:2112	all reduced L:CN cheeses	2089:2112	all reduced L:CN cheeses	2089:2112	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	0	82	from	ratio	131:135	arg1	properties					144:153	the properties	140:153	the properties of milled-curd Cheddar cheese	140:183	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	3	83	theme	excessive	648:656	arg1	acidity					658:664	excessive acidity	648:664	excessive acidity in Cheddar cheese	648:682	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	14	84	theme	casein	2419:2424	arg1	level					2426:2430	a constant casein level	2408:2430	a constant casein level in the milk	2408:2442	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	8	85	theme	due	1376:1378	arg1	moisture					1333:1340	similar moisture	1325:1340	similar moisture	1325:1340	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	13	86	theme	CN	2257:2258	arg1	contents					2260:2267	CN contents	2257:2267	CN contents	2257:2267	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	11	87	theme	significant	1901:1911	arg1	differences					1913:1923	no significant differences	1898:1923	no significant differences	1898:1923	Texture profile analysis showed no significant differences in hardness values during ripening.					
34001358	8	88	theme	slight	1383:1388	arg1	modifications					1390:1402	slight modifications	1383:1402	slight modifications during manufacture	1383:1421	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	7	89	theme	sensory	1227:1233	arg1	properties					1235:1244	sensory properties	1227:1244	sensory properties	1227:1244	These milks were used to make milled-curd Cheddar cheese, and we evaluated cheese composition, texture, functionality, and sensory properties over 9 mo of ripening.					
34001358	3	90	from	acidity	658:664	arg1	cheese					677:682	Cheddar cheese	669:682	Cheddar cheese	669:682	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	6	91	theme	fat	1083:1085	arg1	contents					1094:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	8	92	theme	smaller	1451:1457	arg1	size					1459:1462	a smaller size	1449:1462	a smaller size than control	1449:1475	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	14	93	dep	allow	2451:2455	arg1	maintaining					2396:2406	maintaining	2396:2406	maintaining a constant casein level in the milk	2396:2442	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	13	94	dep	lower	2273:2277	arg1	cohesiveness					2326:2337	chewdown cohesiveness	2317:2337	chewdown cohesiveness	2317:2337	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	13	94	dep	lower	2273:2277	arg1	sourness					2288:2295	sourness	2288:2295	sourness	2288:2295	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	13	94	dep	lower	2273:2277	arg1	notes					2306:2310	sulfury notes	2298:2310	sulfury notes	2298:2310	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	13	94	dep	lower	2273:2277	arg1	acidity					2279:2285	acidity	2279:2285	acidity	2279:2285	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	1	95	theme	cheese	196:201	arg1	pH					190:191	The pH	186:191	The pH of cheese	186:201	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	8	96	dep	gel	1442:1444	arg1	i.e.					1424:1427	i.e.	1424:1427	i.e.	1424:1427	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	4	97	theme	both	870:873	arg1	addition					822:829	the addition	818:829	the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content	818:920	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	0	98	theme	milled-curd	158:168	arg1	cheese					178:183	milled-curd Cheddar cheese	158:183	milled-curd Cheddar cheese	158:183	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	12	99	contain	had	2114:2116	arg1	cheeses					2106:2112	all reduced L:CN cheeses	2089:2112	all reduced L:CN cheeses	2089:2112	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	12	99	contain	had	2114:2116	arg2	values					2146:2151	higher maximum loss tangent values	2118:2151	higher maximum loss tangent values	2118:2151	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	7	100	theme	cheese	1179:1184	arg1	composition					1186:1196	cheese composition	1179:1196	cheese composition	1179:1196	These milks were used to make milled-curd Cheddar cheese, and we evaluated cheese composition, texture, functionality, and sensory properties over 9 mo of ripening.					
34001358	9	101	theme	lower	1637:1641	arg1	acid					1650:1653	lower lactic acid	1637:1653	lower lactic acid	1637:1653	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	0	102	theme	cheese	178:183	arg1	properties					144:153	the properties	140:153	the properties of milled-curd Cheddar cheese	140:183	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	8	103	dep	moisture	1333:1340	arg1	gel					1442:1444	cutting the gel	1430:1444	cutting the gel at a smaller size than control	1430:1475	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	14	104	contain	have	2489:2492	arg2	control					2502:2508	tighter control	2494:2508	tighter control of pH and acidity	2494:2526	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	14	104	contain	have	2489:2492	arg1	manufacturers					2472:2484	Cheddar cheese manufacturers	2457:2484	Cheddar cheese manufacturers	2457:2484	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	12	105	theme	loss	1969:1972	arg1	tangent					1974:1980	Maximum loss tangent	1961:1980	Maximum loss tangent	1961:1980	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	12	105	theme	loss	1969:1972	arg1	index					1986:1990	an index	1983:1990	an index of cheese meltability	1983:2012	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	6	106	theme	total	1059:1063	arg1	casein					1065:1070	total casein	1059:1070	total casein (2.3%)	1059:1077	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	6	106	theme	total	1059:1063	arg1	%					1076:1076	2.3%	1073:1076	2.3%	1073:1076	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	8	107	theme	acid	1500:1503	arg1	development					1505:1515	acid development	1500:1515	acid development	1500:1515	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	108	contain	had	1321:1323	arg1	CN					1318:1319	CN	1318:1319	CN	1318:1319	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	108	contain	had	1321:1323	arg2	control					1489:1495	control	1489:1495	similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development	1325:1515	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	108	contain	had	1321:1323	arg2	moisture					1333:1340	similar moisture	1325:1340	similar moisture	1325:1340	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	108	contain	had	1321:1323	arg2	protein					1343:1349	protein	1343:1349	protein	1343:1349	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	108	contain	had	1321:1323	arg2	contents					1366:1373	salt contents	1361:1373	salt contents	1361:1373	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	108	contain	had	1321:1323	arg2	fat					1352:1354	fat	1352:1354	fat	1352:1354	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	9	109	from	increase	1729:1736	arg1	cheeses					1764:1770	cheeses	1764:1770	cheeses	1764:1770	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	2	110	from	phosphate	428:436	arg1	related					460:466	related	460:466	related	460:466	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	110	from	phosphate	428:436	arg1	curd					445:448	the curd	441:448	the curd	441:448	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	9	111	theme	cheese	1745:1750	arg1	ripening					1752:1759	cheese ripening	1745:1759	cheese ripening	1745:1759	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	10	112	theme	proteolysis	1853:1863	arg1	levels					1821:1826	the levels	1817:1826	the levels of primary and secondary proteolysis	1817:1863	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	14	113	theme	Cheddar	2457:2463	arg1	manufacturers					2472:2484	Cheddar cheese manufacturers	2457:2484	Cheddar cheese manufacturers	2457:2484	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	14	114	theme	acidity	2520:2526	arg1	control					2502:2508	tighter control	2494:2508	tighter control of pH and acidity	2494:2526	Standardization of milk to a specific L:CN ratio, while maintaining a constant casein level in the milk, would allow Cheddar cheese manufacturers to have tighter control of pH and acidity.					
34001358	8	115	theme	L	1316:1316	arg1	levels					1306:1311	varying levels	1298:1311	varying levels of L	1298:1316	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	9	116	theme	insoluble	1678:1686	arg1	contents					1691:1698	insoluble Ca contents	1678:1698	insoluble Ca contents	1678:1698	As expected, decreasing the L:CN led to cheeses with lower lactic acid, residual lactose, and insoluble Ca contents, as well as a substantial pH increase during cheese ripening in cheeses.					
34001358	0	117	theme	lactose-to-casein	113:129	arg1	ratio					131:135	the lactose-to-casein ratio	109:135	the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese	109:183	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	13	118	theme	chewdown	2317:2324	arg1	cohesiveness					2326:2337	chewdown cohesiveness	2317:2337	chewdown cohesiveness	2317:2337	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	7	119	theme	milled-curd	1134:1144	arg1	cheese					1154:1159	milled-curd Cheddar cheese	1134:1159	milled-curd Cheddar cheese	1134:1159	These milks were used to make milled-curd Cheddar cheese, and we evaluated cheese composition, texture, functionality, and sensory properties over 9 mo of ripening.					
34001358	11	120	theme	Texture	1866:1872	arg1	analysis					1882:1889	Texture profile analysis	1866:1889	Texture profile analysis	1866:1889	Texture profile analysis showed no significant differences in hardness values during ripening.					
34001358	12	121	theme	loss	2133:2136	arg1	values					2146:2151	higher maximum loss tangent values	2118:2151	higher maximum loss tangent values	2118:2151	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	2	122	theme	milk	376:379	arg1	milk					376:379	milk	376:379	milk	376:379	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	122	theme	milk	376:379	arg1	contents					364:371	the protein contents	352:371	the protein contents of milk and cheese	352:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	122	theme	milk	376:379	arg1	phosphate					428:436	insoluble calcium phosphate	410:436	insoluble calcium phosphate in the curd, which is related to the rate of acidification	410:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	122	theme	milk	376:379	arg1	cheese					385:390	cheese	385:390	cheese	385:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	122	theme	milk	376:379	arg1	amount					400:405	the amount	396:405	the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification	396:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	5	123	theme	control	997:1003	arg1	1.8					992:994	1.8	992:994	1.8	992:994	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	5	123	theme	control	997:1003	arg1	milk					1005:1008	control milk	997:1008	control milk	997:1008	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	2	124	theme	calcium	420:426	arg1	phosphate					428:436	insoluble calcium phosphate	410:436	insoluble calcium phosphate in the curd, which is related to the rate of acidification	410:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	8	125	theme	similar	1325:1331	arg1	moisture					1333:1340	similar moisture	1325:1340	similar moisture	1325:1340	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	8	126	with	milks	1287:1291	arg1	levels					1306:1311	varying levels	1298:1311	varying levels of L	1298:1316	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	0	127	theme	standardization	18:32	arg1	Effect					0:5	Effect	0:5	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.	0:184	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	10	128	theme	primary	1831:1837	arg1	proteolysis					1853:1863	primary and secondary proteolysis	1831:1863	primary and secondary proteolysis	1831:1863	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	10	129	theme	L	1777:1777	arg1	ratio					1782:1786	The L:CN ratio	1773:1786	The L:CN ratio	1773:1786	The L:CN ratio had no significant effect on the levels of primary and secondary proteolysis.					
34001358	0	130	theme	milk	37:40	arg1	standardization					18:32	lactose standardization	10:32	lactose standardization of milk using low-concentration factor ultrafiltration	10:87	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	1	131	theme	cheese	286:291	arg1	capacity					270:277	the buffering capacity	256:277	the buffering capacity of the cheese	256:291	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	1	131	theme	cheese	286:291	arg1	cheese					286:291	the cheese	282:291	the cheese	282:291	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	1	131	theme	cheese	286:291	arg1	amount					224:229	the amount	220:229	the amount of lactose fermented	220:250	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	1	131	theme	cheese	286:291	arg1	lactose					234:240	lactose	234:240	lactose fermented	234:250	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	4	132	theme	lactose	906:912	arg1	content					914:920	the lactose content	902:920	the lactose content	902:920	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	6	133	contain	had	1047:1049	arg2	contents					1094:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	similar total casein (2.3%) and fat (3.4%) contents	1051:1101	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	6	133	contain	had	1047:1049	arg1	cheesemilks					1035:1045	All cheesemilks	1031:1045	All cheesemilks	1031:1045	All cheesemilks had similar total casein (2.3%) and fat (3.4%) contents.					
34001358	3	134	theme	milk	585:588	arg1	contents					573:580	both the lactose and casein contents	545:580	contents	573:580	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	2	135	theme	cheese	385:390	arg1	milk					376:379	milk	376:379	milk	376:379	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	135	theme	cheese	385:390	arg1	contents					364:371	the protein contents	352:371	the protein contents of milk and cheese	352:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	135	theme	cheese	385:390	arg1	phosphate					428:436	insoluble calcium phosphate	410:436	insoluble calcium phosphate in the curd, which is related to the rate of acidification	410:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	135	theme	cheese	385:390	arg1	cheese					385:390	cheese	385:390	cheese	385:390	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	135	theme	cheese	385:390	arg1	amount					400:405	the amount	396:405	the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification	396:495	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	0	136	theme	low-concentration	48:64	arg1	ultrafiltration					73:87	low-concentration factor ultrafiltration	48:87	low-concentration factor ultrafiltration	48:87	Effect of lactose standardization of milk using low-concentration factor ultrafiltration: Effect of reducing the lactose-to-casein ratio on the properties of milled-curd Cheddar cheese.					
34001358	2	137	from	contents	364:371	arg1	related					460:466	related	460:466	related	460:466	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	2	137	from	contents	364:371	arg1	curd					445:448	the curd	441:448	the curd	441:448	The buffering capacity of cheese is largely determined by the protein contents of milk and cheese and the amount of insoluble calcium phosphate in the curd, which is related to the rate of acidification.					
34001358	13	138	with	milks	2236:2240	arg1	L					2255:2255	reduced L	2247:2255	reduced L	2247:2255	Sensory analyses showed that cheeses made from milks with reduced L:CN contents had lower acidity, sourness, sulfury notes, and chewdown cohesiveness.					
34001358	12	139	theme	CN	2103:2104	arg1	cheeses					2106:2112	all reduced L:CN cheeses	2089:2112	all reduced L:CN cheeses	2089:2112	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	8	140	theme	salt	1361:1364	arg1	contents					1366:1373	salt contents	1361:1373	salt contents	1361:1373	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	3	141	theme	final	608:612	arg1	pH					614:615	final pH	608:615	final pH	608:615	The objective of this study was to standardize both the lactose and casein contents of milk to better control final pH and prevent the development of excessive acidity in Cheddar cheese.					
34001358	4	142	theme	factor	737:742	arg1	ultrafiltration					744:758	low-concentration factor ultrafiltration	719:758	low-concentration factor ultrafiltration of milk	719:766	This approach involved the use of low-concentration factor ultrafiltration of milk to increase the casein content (∼5%), followed by the addition of water, ultrafiltration permeate, or both to the retentate to adjust the lactose content.					
34001358	12	143	dep	cheese	2170:2175	arg1	L					2178:2178	L	2178:2178	L:CN 1.8	2178:2185	Maximum loss tangent, an index of cheese meltability, was lower until 45 d for the L:CN 1.4 and 0.9 treatments, but after 45 d, all reduced L:CN cheeses had higher maximum loss tangent values than the control cheese (L:CN 1.8).					
34001358	5	144	dep	L	985:985	arg1	CN					987:988	CN	987:988	L:CN	985:988	We evaluated milks with 4 different lactose-to-casein ratios (L:CN): 1.8 (control milk), 1.4, 1.1, and 0.9.					
34001358	8	145	theme	cutting	1430:1436	arg1	gel					1442:1444	cutting the gel	1430:1444	cutting the gel at a smaller size than control	1430:1475	Cheeses made from milks with varying levels of L:CN had similar moisture, protein, fat, and salt contents, due to slight modifications during manufacture (i.e., cutting the gel at a smaller size than control) as well as control of acid development at critical steps (i.e., cutting the gel, whey drainage, salting).					
34001358	1	146	theme	lactose	234:240	arg1	capacity					270:277	the buffering capacity	256:277	the buffering capacity of the cheese	256:291	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	1	146	theme	lactose	234:240	arg1	cheese					286:291	the cheese	282:291	the cheese	282:291	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	1	146	theme	lactose	234:240	arg1	amount					224:229	the amount	220:229	the amount of lactose fermented	220:250	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34001358	1	146	theme	lactose	234:240	arg1	lactose					234:240	lactose	234:240	lactose fermented	234:250	The pH of cheese is determined by the amount of lactose fermented and the buffering capacity of the cheese.					
34018184	3	0	theme	napping-UFP	707:717	arg1	exercises					719:727	two napping-UFP exercises	703:727	two napping-UFP exercises	703:727	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	16	1	theme	HSG	3105:3107	arg1	systems					3109:3115	HSG systems	3105:3115	HSG systems	3105:3115	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	12	2	theme	consumer	2125:2132	arg1	demands					2134:2140	Increased consumer demands	2115:2140	Increased consumer demands for nonanimal-derived ingredients	2115:2174	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	2	3	theme	flash	628:632	arg1	profile					634:640	napping-ultra flash profile	614:640	napping-ultra flash profile (napping-UFP)	614:654	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	2	3	theme	flash	628:632	arg1	napping-UFP					643:653	napping-UFP	643:653	napping-UFP	643:653	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	17	4	theme	Additional	3164:3173	arg1	research					3175:3182	Additional research	3164:3182	Additional research	3164:3182	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	6	5	theme	sensory	1229:1235	arg1	descriptors					1237:1247	the sensory descriptors	1225:1247	the sensory descriptors using Spearman's correlation	1225:1276	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	12	6	theme	thermal	2360:2366	arg1	properties					2368:2377	gelatin's textural and thermal properties	2337:2377	gelatin's textural and thermal properties	2337:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	15	7	theme	similar	2828:2834	arg1	structure					2867:2875	the triple-helix structure	2850:2875	the triple-helix structure	2850:2875	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	15	7	theme	similar	2828:2834	arg1	properties					2836:2845	similar properties	2828:2845	similar properties	2828:2845	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	17	8	theme	thermal	3221:3227	arg1	behavior					3229:3236	the thermal behavior	3217:3236	the thermal behavior of gelatin in commercial and model gummy systems	3217:3285	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	13	9	theme	confectionary	2503:2515	arg1	gels					2517:2520	high-solid confectionary gels	2492:2520	high-solid confectionary gels	2492:2520	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	12	10	theme	PRACTICAL	2092:2100	arg1	APPLICATION					2102:2112	PRACTICAL APPLICATION	2092:2112	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.	2092:2378	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	14	11	theme	structural	2623:2632	arg1	element					2634:2640	a powerful structural element	2612:2640	a powerful structural element that determined the textural properties perceived by panelists in HSGs	2612:2711	The triple-helix is a powerful structural element that determined the textural properties perceived by panelists in HSGs.					
34018184	14	11	theme	structural	2623:2632	arg1	triple-helix					2596:2607	triple-helix	2596:2607	triple-helix	2596:2607	The triple-helix is a powerful structural element that determined the textural properties perceived by panelists in HSGs.					
34018184	10	12	theme	small	1708:1712	arg1	structure					1743:1751	a small (3 J/g) gelatin triple-helix structure	1706:1751	a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC)	1706:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	10	13	dep	small	1708:1712	arg1	J/g					1717:1719	3 J/g	1715:1719	3 J/g	1715:1719	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	6	14	theme	analytical	1187:1196	arg1	parameters					1198:1207	the analytical parameters	1183:1207	the analytical parameters	1183:1207	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	6	14	theme	analytical	1187:1196	arg1	related					1214:1220	related	1214:1220	related	1214:1220	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	0	15	theme	clustering	128:137	arg1	analysis					139:146	napping-ultra flash profile (UFP) and hierarchical clustering analysis	77:146	napping-ultra flash profile (UFP) and hierarchical clustering analysis	77:146	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	1	16	theme	confectionary	190:202	arg1	industries					227:236	the confectionary and dietary supplement industries	186:236	the confectionary and dietary supplement industries	186:236	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	2	17	theme	analytical	549:558	arg1	parameters					560:569	analytical parameters	549:569	analytical parameters	549:569	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	11	18	from	desirable	2081:2089	arg1	HSGs					2043:2046	HSGs	2043:2046	HSGs in which gelatin-like texture is desirable	2043:2089	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	12	19	theme	replacement	2266:2276	arg1	material					2278:2285	an economical replacement material	2252:2285	an economical replacement material for gelatin	2252:2297	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	3	20	theme	similarity	807:816	arg1	degree					797:802	their overall degree	783:802	their overall degree of similarity	783:816	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	9	21	theme	measured	1590:1597	arg1	parameters					1610:1619	the measured analytical parameters	1586:1619	the measured analytical parameters	1586:1619	Sensory descriptors generated by panelists during the napping-UFP were significantly correlated to the measured analytical parameters.					
34018184	11	22	from	HSGs	2043:2046	arg1	desirable					2081:2089	desirable	2081:2089	desirable	2081:2089	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	16	23	theme	better	2980:2985	arg1	understanding					2987:2999	a better understanding	2978:2999	a better understanding of the thermal behavior of gelatin	2978:3034	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	15	24	with	products	2896:2903	arg1	texture					2923:2929	gelatin-like texture	2910:2929	gelatin-like texture	2910:2929	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	8	25	theme	samples	1375:1381	arg1	clustering					1357:1366	The clustering	1353:1366	The clustering of the samples across the napping-UFP exercises	1353:1414	The clustering of the samples across the napping-UFP exercises resulted in two categories-gelatin and nongelatin-containing products.					
34018184	0	26	theme	napping-ultra	77:89	arg1	profile					97:103	napping-ultra flash profile	77:103	napping-ultra flash profile (UFP)	77:109	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	0	26	theme	napping-ultra	77:89	arg1	UFP					106:108	UFP	106:108	UFP	106:108	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	2	27	theme	sensory	484:490	arg1	characteristics					492:506	sensory characteristics	484:506	sensory characteristics	484:506	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	12	28	theme	pharmaceutical	2201:2214	arg1	interest					2227:2234	the food and pharmaceutical industries interest	2188:2234	the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties	2188:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	0	29	theme	profile	97:103	arg1	analysis					139:146	napping-ultra flash profile (UFP) and hierarchical clustering analysis	77:146	napping-ultra flash profile (UFP) and hierarchical clustering analysis	77:146	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	1	30	theme	gels	171:174	arg1	use					153:155	The use	149:155	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries	149:236	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	10	31	from	difference	1626:1635	arg1	texture					1640:1646	texture	1640:1646	texture	1640:1646	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	1	32	theme	thermal	365:371	arg1	behavior					373:380	thermal behavior	365:380	thermal behavior	365:380	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	1	33	from	use	153:155	arg1	industries					227:236	the confectionary and dietary supplement industries	186:236	the confectionary and dietary supplement industries	186:236	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	11	34	theme	new	2022:2024	arg1	formulations					2026:2037	new formulations	2022:2037	new formulations for HSGs in which gelatin-like texture is desirable	2022:2089	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	15	35	theme	gelatin-like	2910:2921	arg1	texture					2923:2929	gelatin-like texture	2910:2929	gelatin-like texture	2910:2929	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	8	36	theme	categories-gelatin	1432:1449	arg1	products					1477:1484	two categories-gelatin and nongelatin-containing products	1428:1484	two categories-gelatin and nongelatin-containing products	1428:1484	The clustering of the samples across the napping-UFP exercises resulted in two categories-gelatin and nongelatin-containing products.					
34018184	10	37	theme	structure	1743:1751	arg1	presence					1694:1701	the presence	1690:1701	the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC)	1690:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	13	38	theme	HSGs	2586:2589	arg1	quality					2548:2554	the quality	2544:2554	the quality of current gelatin-containing HSGs	2544:2589	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	6	39	theme	hierarchical	1091:1102	arg1	analysis					1115:1122	hierarchical clustering analysis	1091:1122	hierarchical clustering analysis	1091:1122	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	8	40	theme	nongelatin-containing	1455:1475	arg1	products					1477:1484	two categories-gelatin and nongelatin-containing products	1428:1484	two categories-gelatin and nongelatin-containing products	1428:1484	The clustering of the samples across the napping-UFP exercises resulted in two categories-gelatin and nongelatin-containing products.					
34018184	5	41	theme	water	997:1001	arg1	aw					1013:1014	aw	1013:1014	aw	1013:1014	The products were also characterized by their moisture content, water activity (aw ), thermal behavior, and texture profile.					
34018184	5	41	theme	water	997:1001	arg1	activity					1003:1010	water activity	997:1010	water activity (aw )	997:1016	The products were also characterized by their moisture content, water activity (aw ), thermal behavior, and texture profile.					
34018184	12	42	theme	food	2192:2195	arg1	interest					2227:2234	the food and pharmaceutical industries interest	2188:2234	the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties	2188:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	17	43	theme	model	3267:3271	arg1	systems					3279:3285	model gummy systems	3267:3285	model gummy systems	3267:3285	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	4	44	theme	factor	849:854	arg1	analysis					856:863	Multiple factor analysis	840:863	Multiple factor analysis	840:863	Multiple factor analysis and hierarchical clustering were conducted on the napping-UFP data.					
34018184	10	45	theme	differential	1770:1781	arg1	DSC					1805:1807	DSC	1805:1807	DSC	1805:1807	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	10	45	theme	differential	1770:1781	arg1	calorimetry					1792:1802	differential scanning calorimetry	1770:1802	differential scanning calorimetry (DSC)	1770:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	0	46	theme	commercial	39:48	arg1	gels					64:67	commercial confectionary gels	39:67	commercial confectionary gels	39:67	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	5	47	theme	texture	1041:1047	arg1	profile					1049:1055	texture profile	1041:1055	texture profile	1041:1055	The products were also characterized by their moisture content, water activity (aw ), thermal behavior, and texture profile.					
34018184	6	48	theme	Principal	1058:1066	arg1	analysis					1078:1085	Principal component analysis	1058:1085	Principal component analysis	1058:1085	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	2	49	theme	product	456:462	arg1	categories					464:473	HSG product categories	452:473	HSG product categories based on sensory characteristics and analytical parameters	452:532	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	14	50	from	panelists	2695:2703	arg1	HSGs					2708:2711	HSGs	2708:2711	HSGs	2708:2711	The triple-helix is a powerful structural element that determined the textural properties perceived by panelists in HSGs.					
34018184	3	51	theme	90-min	664:669	arg1	session					671:677	one 90-min session	660:677	one 90-min session	660:677	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	12	52	theme	textural	2347:2354	arg1	properties					2368:2377	gelatin's textural and thermal properties	2337:2377	gelatin's textural and thermal properties	2337:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	0	53	theme	gels	64:67	arg1	categorization					21:34	categorization	21:34	categorization	21:34	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	0	53	theme	gels	64:67	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	13	54	theme	current	2559:2565	arg1	HSGs					2586:2589	current gelatin-containing HSGs	2559:2589	current gelatin-containing HSGs	2559:2589	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	10	55	dep	structure	1743:1751	arg1	determined					1756:1765	determined	1756:1765	determined by differential scanning calorimetry (DSC)	1756:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	11	56	theme	sensory	1938:1944	arg1	characteristics					1946:1960	different sensory characteristics	1928:1960	different sensory characteristics than gelatin	1928:1973	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	1	57	theme	supplement	216:225	arg1	industries					227:236	the confectionary and dietary supplement industries	186:236	the confectionary and dietary supplement industries	186:236	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	5	58	theme	thermal	1019:1025	arg1	behavior					1027:1034	thermal behavior	1019:1034	thermal behavior	1019:1034	The products were also characterized by their moisture content, water activity (aw ), thermal behavior, and texture profile.					
34018184	11	59	theme	important	1984:1992	arg1	implications					1994:2005	important implications	1984:2005	important implications for developing new formulations for HSGs in which gelatin-like texture is desirable	1984:2089	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	9	60	theme	Sensory	1487:1493	arg1	descriptors					1495:1505	Sensory descriptors	1487:1505	Sensory descriptors generated by panelists during the napping-UFP	1487:1551	Sensory descriptors generated by panelists during the napping-UFP were significantly correlated to the measured analytical parameters.					
34018184	12	61	theme	nonanimal-derived	2146:2162	arg1	ingredients					2164:2174	nonanimal-derived ingredients	2146:2174	nonanimal-derived ingredients	2146:2174	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	16	62	theme	systems	3109:3115	arg1	stability					3069:3077	stability	3069:3077	stability	3069:3077	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	16	62	theme	systems	3109:3115	arg1	functionality					3054:3066	functionality	3054:3066	functionality	3054:3066	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	16	62	theme	systems	3109:3115	arg1	integrity					3092:3100	product integrity	3084:3100	product integrity	3084:3100	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	15	63	theme	replacement	2745:2755	arg1	material					2757:2764	a replacement material	2743:2764	a replacement material for gelatin	2743:2776	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	14	64	theme	textural	2662:2669	arg1	properties					2671:2680	the textural properties	2658:2680	the textural properties perceived by panelists in HSGs	2658:2711	The triple-helix is a powerful structural element that determined the textural properties perceived by panelists in HSGs.					
34018184	17	65	theme	gummy	3273:3277	arg1	systems					3279:3285	model gummy systems	3267:3285	model gummy systems	3267:3285	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	3	66	dep	performed	693:701	arg1	describing					747:756	describing	747:756	describing 13 confectionary HSGs by their overall degree of similarity and by their texture	747:837	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	3	66	dep	performed	693:701	arg1	categorizing					730:741	categorizing	730:741	categorizing	730:741	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	12	67	theme	Increased	2115:2123	arg1	demands					2134:2140	Increased consumer demands	2115:2140	Increased consumer demands for nonanimal-derived ingredients	2115:2174	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	2	68	theme	napping-ultra	614:626	arg1	profile					634:640	napping-ultra flash profile	614:640	napping-ultra flash profile (napping-UFP)	614:654	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	2	68	theme	napping-ultra	614:626	arg1	napping-UFP					643:653	napping-UFP	643:653	napping-UFP	643:653	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	13	69	theme	important	2414:2422	arg1	implications					2424:2435	important implications	2414:2435	important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs	2414:2589	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	3	70	theme	confectionary	761:773	arg1	HSGs					775:778	13 confectionary HSGs	758:778	13 confectionary HSGs	758:778	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	13	71	theme	high-solid	2492:2501	arg1	gels					2517:2520	high-solid confectionary gels	2492:2520	high-solid confectionary gels	2492:2520	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	4	72	theme	hierarchical	869:880	arg1	clustering					882:891	hierarchical clustering	869:891	hierarchical clustering	869:891	Multiple factor analysis and hierarchical clustering were conducted on the napping-UFP data.					
34018184	16	73	theme	behavior	3016:3023	arg1	understanding					2987:2999	a better understanding	2978:2999	a better understanding of the thermal behavior of gelatin	2978:3034	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	16	74	theme	gelatin	3028:3034	arg1	behavior					3016:3023	the thermal behavior	3004:3023	the thermal behavior of gelatin	3004:3034	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	13	75	theme	gelatin-containing	2567:2584	arg1	HSGs					2586:2589	current gelatin-containing HSGs	2559:2589	current gelatin-containing HSGs	2559:2589	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	3	76	theme	overall	789:795	arg1	degree					797:802	their overall degree	783:802	their overall degree of similarity	783:816	In one 90-min session, 15 panelists performed two napping-UFP exercises, categorizing and describing 13 confectionary HSGs by their overall degree of similarity and by their texture.					
34018184	17	77	theme	gelatin	3241:3247	arg1	behavior					3229:3236	the thermal behavior	3217:3236	the thermal behavior of gelatin in commercial and model gummy systems	3217:3285	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	10	78	theme	gelatin	1722:1728	arg1	structure					1743:1751	a small (3 J/g) gelatin triple-helix structure	1706:1751	a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC)	1706:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	11	79	theme	modified	1893:1900	arg1	starch					1902:1907	modified starch	1893:1907	modified starch	1893:1907	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	16	80	theme	thermal	3008:3014	arg1	behavior					3016:3023	the thermal behavior	3004:3023	the thermal behavior of gelatin	3004:3034	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	12	81	theme	economical	2255:2264	arg1	material					2278:2285	an economical replacement material	2252:2285	an economical replacement material for gelatin	2252:2297	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	11	82	theme	different	1928:1936	arg1	characteristics					1946:1960	different sensory characteristics	1928:1960	different sensory characteristics than gelatin	1928:1973	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	16	83	theme	product	3084:3090	arg1	integrity					3092:3100	product integrity	3084:3100	product integrity	3084:3100	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	11	84	theme	gelatin-like	2057:2068	arg1	texture					2070:2076	gelatin-like texture	2057:2076	gelatin-like texture	2057:2076	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	9	85	theme	analytical	1599:1608	arg1	parameters					1610:1619	the measured analytical parameters	1586:1619	the measured analytical parameters	1586:1619	Sensory descriptors generated by panelists during the napping-UFP were significantly correlated to the measured analytical parameters.					
34018184	12	86	theme	industries	2216:2225	arg1	interest					2227:2234	the food and pharmaceutical industries interest	2188:2234	the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties	2188:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	13	87	theme	suitable	2449:2456	arg1	ingredients					2458:2468	suitable ingredients	2449:2468	suitable ingredients	2449:2468	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	2	88	theme	sensory	576:582	arg1	descriptors					584:594	sensory descriptors	576:594	sensory descriptors generated through napping-ultra flash profile (napping-UFP)	576:654	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	4	89	theme	napping-UFP	915:925	arg1	data					927:930	the napping-UFP data	911:930	the napping-UFP data	911:930	Multiple factor analysis and hierarchical clustering were conducted on the napping-UFP data.					
34018184	0	90	theme	flash	91:95	arg1	profile					97:103	napping-ultra flash profile	77:103	napping-ultra flash profile (UFP)	77:109	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	0	90	theme	flash	91:95	arg1	UFP					106:108	UFP	106:108	UFP	106:108	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	13	91	theme	research	2400:2407	arg1	results					2384:2390	The results	2380:2390	The results of this research	2380:2407	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	1	92	theme	sensory	340:346	arg1	characteristics					348:362	sensory characteristics	340:362	sensory characteristics	340:362	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	1	93	theme	high-solid	160:169	arg1	HSGs					177:180	HSGs	177:180	HSGs	177:180	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	1	93	theme	high-solid	160:169	arg1	gels					171:174	high-solid gels	160:174	high-solid gels (HSGs)	160:181	The use of high-solid gels (HSGs) in the confectionary and dietary supplement industries is rapidly expanding, increasing the interest in studying the relationships across their composition, sensory characteristics, thermal behavior, and texture.					
34018184	15	94	theme	triple-helix	2854:2865	arg1	structure					2867:2875	the triple-helix structure	2850:2875	the triple-helix structure	2850:2875	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	15	94	theme	triple-helix	2854:2865	arg1	properties					2836:2845	similar properties	2828:2845	similar properties	2828:2845	Consequently, the search for a replacement material for gelatin should focus on identifying materials that impart similar properties as the triple-helix structure, helping to create products with gelatin-like texture.					
34018184	8	95	theme	napping-UFP	1394:1404	arg1	exercises					1406:1414	the napping-UFP exercises	1390:1414	the napping-UFP exercises	1390:1414	The clustering of the samples across the napping-UFP exercises resulted in two categories-gelatin and nongelatin-containing products.					
34018184	0	96	theme	hierarchical	115:126	arg1	clustering					128:137	hierarchical clustering	115:137	hierarchical clustering	115:137	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	12	97	link	nonanimal-derived	2146:2162	arg1	ingredients					2164:2174	nonanimal-derived ingredients	2146:2174	nonanimal-derived ingredients	2146:2174	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	2	98	theme	analytical	512:521	arg1	parameters					523:532	analytical parameters	512:532	analytical parameters	512:532	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	12	99	dep	APPLICATION	2102:2112	arg1	fueled					2181:2186	fueled	2181:2186	have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties	2176:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	13	100	contain	have	2409:2412	arg1	results					2384:2390	The results	2380:2390	The results of this research	2380:2407	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	13	100	contain	have	2409:2412	arg2	implications					2424:2435	important implications	2414:2435	important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs	2414:2589	The results of this research have important implications for finding suitable ingredients to replace gelatin in high-solid confectionary gels, as well as enhancing the quality of current gelatin-containing HSGs.					
34018184	5	101	theme	moisture	979:986	arg1	content					988:994	their moisture content	973:994	their moisture content	973:994	The products were also characterized by their moisture content, water activity (aw ), thermal behavior, and texture profile.					
34018184	6	102	theme	clustering	1104:1113	arg1	analysis					1115:1122	hierarchical clustering analysis	1091:1122	hierarchical clustering analysis	1091:1122	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	10	103	theme	triple-helix	1730:1741	arg1	structure					1743:1751	a small (3 J/g) gelatin triple-helix structure	1706:1751	a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC)	1706:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	11	104	contain	have	1979:1982	arg1	starch					1902:1907	modified starch	1893:1907	modified starch	1893:1907	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	11	104	contain	have	1979:1982	arg2	implications					1994:2005	important implications	1984:2005	important implications for developing new formulations for HSGs in which gelatin-like texture is desirable	1984:2089	These findings indicate that modified starch and pectin, impart different sensory characteristics than gelatin and have important implications for developing new formulations for HSGs in which gelatin-like texture is desirable.					
34018184	2	105	theme	study	425:429	arg1	objectives					406:415	the objectives	402:415	the objectives of this study	402:429	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	12	106	theme	properties	2368:2377	arg1	understanding					2320:2332	a deeper understanding	2311:2332	a deeper understanding of gelatin's textural and thermal properties	2311:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
34018184	10	107	theme	scanning	1783:1790	arg1	DSC					1805:1807	DSC	1805:1807	DSC	1805:1807	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	10	107	theme	scanning	1783:1790	arg1	calorimetry					1792:1802	differential scanning calorimetry	1770:1802	differential scanning calorimetry (DSC)	1770:1808	The difference in texture between the two clusters, associated with the presence of a small (3 J/g) gelatin triple-helix structure as determined by differential scanning calorimetry (DSC), was significant enough to be perceived by panelists.					
34018184	0	108	theme	confectionary	50:62	arg1	gels					64:67	commercial confectionary gels	39:67	commercial confectionary gels	39:67	Characterization and categorization of commercial confectionary gels through napping-ultra flash profile (UFP) and hierarchical clustering analysis.					
34018184	6	109	theme	component	1068:1076	arg1	analysis					1078:1085	Principal component analysis	1058:1085	Principal component analysis	1058:1085	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	4	110	theme	Multiple	840:847	arg1	analysis					856:863	Multiple factor analysis	840:863	Multiple factor analysis	840:863	Multiple factor analysis and hierarchical clustering were conducted on the napping-UFP data.					
34018184	14	111	theme	powerful	2614:2621	arg1	element					2634:2640	a powerful structural element	2612:2640	a powerful structural element that determined the textural properties perceived by panelists in HSGs	2612:2711	The triple-helix is a powerful structural element that determined the textural properties perceived by panelists in HSGs.					
34018184	14	111	theme	powerful	2614:2621	arg1	triple-helix					2596:2607	triple-helix	2596:2607	triple-helix	2596:2607	The triple-helix is a powerful structural element that determined the textural properties perceived by panelists in HSGs.					
34018184	2	112	theme	HSG	452:454	arg1	categories					464:473	HSG product categories	452:473	HSG product categories based on sensory characteristics and analytical parameters	452:532	Thus, the objectives of this study were to characterize HSG product categories based on sensory characteristics and analytical parameters, and correlate analytical parameters with sensory descriptors generated through napping-ultra flash profile (napping-UFP).					
34018184	16	113	dep	functionality	3054:3066	arg1	the					3050:3052	the	3050:3052	the	3050:3052	Additionally, these findings begin to provide a better understanding of the thermal behavior of gelatin for enhancing the functionality, stability, and product integrity of HSG systems throughout shipping, storage, and consumption.					
34018184	17	114	from	behavior	3229:3236	arg1	commercial					3252:3261	commercial	3252:3261	commercial	3252:3261	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	17	114	from	behavior	3229:3236	arg1	systems					3279:3285	model gummy systems	3267:3285	model gummy systems	3267:3285	Additional research is underway to explore in detail the thermal behavior of gelatin in commercial and model gummy systems.					
34018184	6	115	theme	analytical	1146:1155	arg1	parameters					1157:1166	the analytical parameters	1142:1166	the analytical parameters	1142:1166	Principal component analysis and hierarchical clustering analysis were conducted on the analytical parameters; additionally, the analytical parameters were related to the sensory descriptors using Spearman's correlation.					
34018184	12	116	theme	deeper	2313:2318	arg1	understanding					2320:2332	a deeper understanding	2311:2332	a deeper understanding of gelatin's textural and thermal properties	2311:2377	PRACTICAL APPLICATION: Increased consumer demands for nonanimal-derived ingredients have fueled the food and pharmaceutical industries interest in both finding an economical replacement material for gelatin and gaining a deeper understanding of gelatin's textural and thermal properties.					
33137584	8	0	theme	Km	1254:1255	arg1	values					1257:1262	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL	1250:1307	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL	1250:1307	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	0	1	theme	forms	81:85	arg1	deactivation					8:19	Thermal deactivation	0:19	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.	0:121	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	3	2	theme	immobilized	474:484	arg1	enzymes					486:492	all the immobilized enzymes	466:492	all the immobilized enzymes	466:492	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	8	3	theme	Vmax	1313:1316	arg1	values					1318:1323	Vmax values	1313:1323	Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E	1313:1414	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	0	4	theme	kinetic	90:96	arg1	study					116:120	A kinetic and thermodynamic study	88:120	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.	0:121	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	8	5	theme	16.39 ± 0.01 μmol mg-1	1349:1370	arg1	CSM-GLY-E					1381:1389	16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E	1349:1389	16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E	1349:1389	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	9	6	theme	good	1555:1558	arg1	potential					1566:1574	very good reuse potential	1550:1574	very good reuse potential	1550:1574	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	5	7	theme	order	904:908	arg1	kinetics					910:917	the first order kinetics	894:917	the first order kinetics	894:917	The thermal deactivation of the free and immobilized enzymes follows the first order kinetics.					
33137584	4	8	theme	immobilized	681:691	arg1	enzymes					693:699	covalently immobilized enzymes	670:699	covalently immobilized enzymes as enzyme	670:709	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	9	9	theme	storage	1538:1544	arg1	days					1530:1533	90 days	1527:1533	90 days of storage and very good reuse potential	1527:1574	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	4	10	dep	50	641:642	arg1	to					638:639	to	638:639	to	638:639	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	1	11	dep	stability	127:135	arg1	The					123:125	The	123:125	The	123:125	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	4	12	theme	activity	657:664	arg1	%					643:643	up to 50%	635:643	up to 50% of relative activity	635:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	4	12	theme	activity	657:664	arg1	activity					657:664	relative activity	648:664	relative activity	648:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	4	12	theme	activity	657:664	arg1	stability					613:621	The temperature stability	597:621	The temperature stability at 60 °C	597:630	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	0	13	theme	thermodynamic	102:114	arg1	study					116:120	A kinetic and thermodynamic study	88:120	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.	0:121	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	5	14	theme	free	857:860	arg1	enzymes					878:884	the free and immobilized enzymes	853:884	the free and immobilized enzymes	853:884	The thermal deactivation of the free and immobilized enzymes follows the first order kinetics.					
33137584	3	15	theme	improved	504:511	arg1	stability					516:524	improved pH stability	504:524	improved pH stability	504:524	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	4	16	from	60 °C	626:630	arg1	%					643:643	up to 50%	635:643	up to 50% of relative activity	635:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	4	16	from	60 °C	626:630	arg1	activity					657:664	relative activity	648:664	relative activity	648:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	4	16	from	60 °C	626:630	arg1	stability					613:621	The temperature stability	597:621	The temperature stability at 60 °C	597:630	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	8	17	dep	values	1318:1323	arg1	CSM-ECH-E					1406:1414	CSM-ECH-E	1406:1414	CSM-ECH-E	1406:1414	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	8	17	dep	values	1318:1323	arg1	19.6 ± 0.02					1336:1346	19.6 ± 0.02	1336:1346	19.6 ± 0.02	1336:1346	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	8	17	dep	values	1318:1323	arg1	CSM-GLA-E					1392:1400	CSM-GLA-E	1392:1400	CSM-GLA-E	1392:1400	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	8	17	dep	values	1318:1323	arg1	CSM-GLY-E					1381:1389	16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E	1349:1389	16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E	1349:1389	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	8	17	dep	values	1318:1323	arg1	25 ± 0.06					1325:1333	25 ± 0.06	1325:1333	25 ± 0.06	1325:1333	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	7	18	theme	Ed	1090:1091	arg1	values					1093:1098	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E	1086:1182	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E	1086:1182	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	18	theme	Ed	1090:1091	arg1	CSM-ECH-E					1174:1182	CSM-ECH-E	1174:1182	CSM-ECH-E	1174:1182	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	18	theme	Ed	1090:1091	arg1	CSM-GLA-E					1160:1168	CSM-GLA-E	1160:1168	CSM-GLA-E	1160:1168	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	18	theme	Ed	1090:1091	arg1	KJmol-1					1130:1136	18.71, 32.00, 27.19 and 20.46 KJmol-1	1100:1136	18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E	1100:1146	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	18	theme	Ed	1090:1091	arg1	CSM-GLY-E					1149:1157	CSM-GLY-E	1149:1157	CSM-GLY-E	1149:1157	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	4	19	theme	conformational	775:788	arg1	changes					790:796	the conformational changes	771:796	the conformational changes caused by the environment	771:822	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	9	20	theme	potential	1566:1574	arg1	days					1530:1533	90 days	1527:1533	90 days of storage and very good reuse potential	1527:1574	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	3	21	theme	pH	513:514	arg1	stability					516:524	improved pH stability	504:524	improved pH stability	504:524	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	1	22	theme	enzyme	172:177	arg1	factors					192:198	the main factors	183:198	the main factors	183:198	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	1	22	theme	enzyme	172:177	arg1	stability					127:135	stability	127:135	stability	127:135	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	1	22	theme	enzyme	172:177	arg1	properties					155:164	thermodynamic properties	141:164	thermodynamic properties	141:164	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	7	23	dep	values	1093:1098	arg1	CSM-ECH-E					1174:1182	CSM-ECH-E	1174:1182	CSM-ECH-E	1174:1182	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	23	dep	values	1093:1098	arg1	KJmol-1					1130:1136	18.71, 32.00, 27.19 and 20.46 KJmol-1	1100:1136	18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E	1100:1146	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	23	dep	values	1093:1098	arg1	CSM-GLA-E					1160:1168	CSM-GLA-E	1160:1168	CSM-GLA-E	1160:1168	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	23	dep	values	1093:1098	arg1	values					1093:1098	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E	1086:1182	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E	1086:1182	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	7	23	dep	values	1093:1098	arg1	CSM-GLY-E					1149:1157	CSM-GLY-E	1149:1157	CSM-GLY-E	1149:1157	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	2	24	theme	chitosan-magnetite	316:333	arg1	composite					341:349	synthesized chitosan-magnetite (CSM) composite	304:349	synthesized chitosan-magnetite (CSM) composite	304:349	With that intention we have immobilized the α-amylase onto synthesized chitosan-magnetite (CSM) composite and its modified forms by gluteraldehyde (CSM-GLA), glyoxal (CSM-GLY) and epichlorohydrin (CSM-ECH).					
33137584	7	25	theme	high	1198:1201	arg1	stability					1203:1211	the high stability	1194:1211	the high stability	1194:1211	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	6	26	theme	better	1024:1029	arg1	stability					1039:1047	better thermal stability	1024:1047	better thermal stability than that of free and adsorbed ones	1024:1083	The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones.					
33137584	0	27	theme	Thermal	0:6	arg1	deactivation					8:19	Thermal deactivation	0:19	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.	0:121	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	3	28	theme	relative	542:549	arg1	activity					551:558	relative activity	542:558	relative activity at pH 9	542:566	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	0	29	theme	α-amylase	24:32	arg1	chitosan					55:62	immobilized magnetic chitosan	34:62	immobilized magnetic chitosan	34:62	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	5	30	theme	enzymes	878:884	arg1	deactivation					837:848	The thermal deactivation	825:848	The thermal deactivation of the free and immobilized enzymes	825:884	The thermal deactivation of the free and immobilized enzymes follows the first order kinetics.					
33137584	5	31	theme	first	898:902	arg1	kinetics					910:917	the first order kinetics	894:917	the first order kinetics	894:917	The thermal deactivation of the free and immobilized enzymes follows the first order kinetics.					
33137584	1	32	theme	main	187:190	arg1	factors					192:198	the main factors	183:198	the main factors	183:198	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	1	32	theme	main	187:190	arg1	stability					127:135	stability	127:135	stability	127:135	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	1	32	theme	main	187:190	arg1	properties					155:164	thermodynamic properties	141:164	thermodynamic properties	141:164	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	2	33	theme	synthesized	304:314	arg1	composite					341:349	synthesized chitosan-magnetite (CSM) composite	304:349	synthesized chitosan-magnetite (CSM) composite	304:349	With that intention we have immobilized the α-amylase onto synthesized chitosan-magnetite (CSM) composite and its modified forms by gluteraldehyde (CSM-GLA), glyoxal (CSM-GLY) and epichlorohydrin (CSM-ECH).					
33137584	9	34	theme	activity	1512:1519	arg1	activity					1512:1519	relative activity	1503:1519	relative activity	1503:1519	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	9	34	theme	activity	1512:1519	arg1	%					1498:1498	about 60%	1490:1498	about 60% of relative activity	1490:1519	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	3	35	dep	exhibited	494:502	arg1	compared					568:575	compared	568:575	compared to the free enzyme	568:594	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	3	36	from	pH	563:564	arg1	%					537:537	60-80%	532:537	60-80% of relative activity at pH 9	532:566	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	3	36	from	pH	563:564	arg1	activity					551:558	relative activity	542:558	relative activity at pH 9	542:566	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	6	37	theme	free	1062:1065	arg1	ones					1080:1083	free and adsorbed ones	1062:1083	free and adsorbed ones	1062:1083	The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones.					
33137584	0	38	theme	magnetic	46:53	arg1	chitosan					55:62	immobilized magnetic chitosan	34:62	immobilized magnetic chitosan	34:62	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	6	39	theme	immobilized	992:1002	arg1	enzymes					1004:1010	covalently immobilized enzymes	981:1010	covalently immobilized enzymes	981:1010	The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones.					
33137584	8	40	dep	values	1257:1262	arg1	0.525 ± 0.04					1264:1275	0.525 ± 0.04	1264:1275	0.525 ± 0.04	1264:1275	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	8	40	dep	values	1257:1262	arg1	0.65 ± 0.04 mg/mL					1291:1307	0.65 ± 0.04 mg/mL	1291:1307	0.65 ± 0.04 mg/mL	1291:1307	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	4	41	theme	temperature	601:611	arg1	%					643:643	up to 50%	635:643	up to 50% of relative activity	635:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	4	41	theme	temperature	601:611	arg1	activity					657:664	relative activity	648:664	relative activity	648:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	4	41	theme	temperature	601:611	arg1	stability					613:621	The temperature stability	597:621	The temperature stability at 60 °C	597:630	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	0	42	theme	immobilized	34:44	arg1	chitosan					55:62	immobilized magnetic chitosan	34:62	immobilized magnetic chitosan	34:62	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	3	43	theme	activity	551:558	arg1	%					537:537	60-80%	532:537	60-80% of relative activity at pH 9	532:566	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	3	43	theme	activity	551:558	arg1	activity					551:558	relative activity	542:558	relative activity at pH 9	542:566	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	4	44	theme	relative	648:655	arg1	activity					657:664	relative activity	648:664	relative activity	648:664	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	0	45	theme	chitosan	55:62	arg1	deactivation					8:19	Thermal deactivation	0:19	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.	0:121	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	8	46	theme	substrate	1431:1439	arg1	affinity					1441:1448	better substrate affinity	1424:1448	better substrate affinity	1424:1448	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	8	47	theme	better	1424:1429	arg1	affinity					1441:1448	better substrate affinity	1424:1448	better substrate affinity	1424:1448	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	9	48	theme	immobilized	1455:1465	arg1	enzymes					1467:1473	The immobilized enzymes	1451:1473	The immobilized enzymes	1451:1473	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	8	49	theme	min-1for	1372:1379	arg1	CSM-GLY-E					1381:1389	16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E	1349:1389	16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E	1349:1389	The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity.					
33137584	4	50	theme	covalent	732:739	arg1	binding					741:747	covalent binding	732:747	covalent binding	732:747	The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment.					
33137584	5	51	theme	immobilized	866:876	arg1	enzymes					878:884	the free and immobilized enzymes	853:884	the free and immobilized enzymes	853:884	The thermal deactivation of the free and immobilized enzymes follows the first order kinetics.					
33137584	3	52	from	%	537:537	arg1	pH					563:564	pH 9	563:566	pH 9	563:566	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	6	53	dep	t1/2	924:927	arg1	The					920:922	The	920:922	The	920:922	The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones.					
33137584	3	54	theme	free	584:587	arg1	enzyme					589:594	the free enzyme	580:594	the free enzyme	580:594	In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme.					
33137584	1	55	theme	thermodynamic	141:153	arg1	factors					192:198	the main factors	183:198	the main factors	183:198	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	1	55	theme	thermodynamic	141:153	arg1	stability					127:135	stability	127:135	stability	127:135	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	1	55	theme	thermodynamic	141:153	arg1	properties					155:164	thermodynamic properties	141:164	thermodynamic properties	141:164	The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry.					
33137584	6	56	theme	adsorbed	1071:1078	arg1	ones					1080:1083	free and adsorbed ones	1062:1083	free and adsorbed ones	1062:1083	The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones.					
33137584	0	57	theme	modified	72:79	arg1	forms					81:85	its modified forms	68:85	its modified forms	68:85	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	0	58	dep	deactivation	8:19	arg1	study					116:120	A kinetic and thermodynamic study	88:120	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.	0:121	Thermal deactivation of α-amylase immobilized magnetic chitosan and its modified forms: A kinetic and thermodynamic study.					
33137584	7	59	theme	heat	1231:1234	arg1	inactivation					1236:1247	heat inactivation	1231:1247	heat inactivation	1231:1247	The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation.					
33137584	5	60	theme	thermal	829:835	arg1	deactivation					837:848	The thermal deactivation	825:848	The thermal deactivation of the free and immobilized enzymes	825:884	The thermal deactivation of the free and immobilized enzymes follows the first order kinetics.					
33137584	6	61	theme	thermal	1031:1037	arg1	stability					1039:1047	better thermal stability	1024:1047	better thermal stability than that of free and adsorbed ones	1024:1083	The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones.					
33137584	9	62	theme	relative	1503:1510	arg1	activity					1512:1519	relative activity	1503:1519	relative activity	1503:1519	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
33137584	2	63	theme	modified	359:366	arg1	forms					368:372	its modified forms	355:372	its modified forms	355:372	With that intention we have immobilized the α-amylase onto synthesized chitosan-magnetite (CSM) composite and its modified forms by gluteraldehyde (CSM-GLA), glyoxal (CSM-GLY) and epichlorohydrin (CSM-ECH).					
33137584	9	64	theme	reuse	1560:1564	arg1	potential					1566:1574	very good reuse potential	1550:1574	very good reuse potential	1550:1574	The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.					
32943084	4	0	theme	structural	886:895	arg1	changes					897:903	structural changes	886:903	structural changes on CR	886:909	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	3	1	theme	GAG	698:700	arg1	transfer					731:738	the compositional GAG chemical exchange saturation transfer	680:738	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	12	2	theme	disease-related	1982:1996	arg1	findings					2015:2022	disease-related pathological MRI findings	1982:2022	disease-related pathological MRI findings	1982:2022	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	12	3	theme	MRI	2011:2013	arg1	findings					2015:2022	disease-related pathological MRI findings	1982:2022	disease-related pathological MRI findings	1982:2022	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	4	4	theme	disk	863:866	arg1	degeneration					868:879	disk degeneration	863:879	disk degeneration	863:879	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	1	5	with	volunteers	309:318	arg1	spondylitis					280:290	ankylosing spondylitis	269:290	ankylosing spondylitis (AS)	269:295	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	8	6	theme	AS	1239:1240	arg1	patients					1242:1249	AS patients	1239:1249	AS patients (2.0 ± 1.7%)	1239:1262	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	8	6	theme	AS	1239:1240	arg1	%					1261:1261	2.0 ± 1.7%	1252:1261	2.0 ± 1.7%	1252:1261	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	11	7	theme	CONCLUSIONS	1605:1615	arg1	spines					1624:1629	CONCLUSIONS Lumbar spines	1605:1629	CONCLUSIONS Lumbar spines of r-AS patients	1605:1646	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	9	8	theme	CRP	1386:1388	arg1	levels					1390:1395	CRP levels	1386:1395	CRP levels	1386:1395	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	11	9	theme	serological	1775:1785	arg1	markers					1800:1806	increased serological inflammation markers	1765:1806	increased serological inflammation markers	1765:1806	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	10	10	dep	BASDAI	1552:1557	arg1	scores					1597:1602	MRI scores	1593:1602	MRI scores	1593:1602	No significant correlations were found with BASDAI, inflammation, and fat deposition MRI scores.					
32943084	8	11	theme	GagCEST	1196:1202	arg1	values					1204:1209	GagCEST values	1196:1209	GagCEST values	1196:1209	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	6	12	theme	mixed	1126:1130	arg1	model					1132:1136	a linear mixed model	1117:1136	a linear mixed model	1117:1136	GagCEST values of both groups were compared using a linear mixed model.					
32943084	0	13	theme	lumbar	142:147	arg1	spine					149:153	the lumbar spine	138:153	the lumbar spine	138:153	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	1	14	theme	clinical	392:399	arg1	features					413:420	disease-related clinical and imaging features	376:420	disease-related clinical and imaging features	376:420	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	1	15	theme	lumbar	217:222	arg1	IVDs					246:249	IVDs	246:249	IVDs	246:249	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	1	15	theme	lumbar	217:222	arg1	disks					239:243	lumbar intervertebral disks	217:243	lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers	217:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	2	16	theme	magnetic	542:549	arg1	MRI					570:572	MRI	570:572	MRI	570:572	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	16	theme	magnetic	542:549	arg1	imaging					561:567	3-T magnetic resonance imaging	538:567	3-T magnetic resonance imaging (MRI)	538:573	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	1	17	theme	imaging	405:411	arg1	features					413:420	disease-related clinical and imaging features	376:420	disease-related clinical and imaging features	376:420	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	4	18	from	deposition	851:860	arg1	CR					908:909	CR	908:909	CR	908:909	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	1	19	theme	disks	239:243	arg1	content					206:212	the glycosaminoglycan (GAG) content	178:212	the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers	178:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	12	20	theme	confirmatory	1943:1954	arg1	role					1956:1959	an adjunct confirmatory role	1932:1959	an adjunct confirmatory role	1932:1959	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	12	21	from	role	1850:1853	arg1	AS					1875:1876	AS	1875:1876	AS	1875:1876	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	5	22	theme	C-reactive	1034:1043	arg1	CRP					1054:1056	CRP	1054:1056	CRP	1054:1056	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	5	22	theme	C-reactive	1034:1043	arg1	protein					1045:1051	C-reactive protein	1034:1051	C-reactive protein (CRP) levels	1034:1064	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	1	23	dep	spondylitis	280:290	arg1	AS					293:294	AS	293:294	AS	293:294	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	11	24	theme	syndesmophyte	1713:1725	arg1	formation					1727:1735	syndesmophyte formation	1713:1735	syndesmophyte formation	1713:1735	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	3	25	theme	MRI	614:616	arg1	protocol					618:625	The MRI protocol	610:625	The MRI protocol	610:625	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	2	26	theme	age-matched	497:507	arg1	volunteers					509:518	30 age-matched volunteers	494:518	30 age-matched volunteers	494:518	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	9	27	theme	syndesmophytes	1468:1481	arg1	values					1375:1380	gagCEST values	1367:1380	gagCEST values	1367:1380	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	9	27	theme	syndesmophytes	1468:1481	arg1	levels					1390:1395	CRP levels	1386:1395	CRP levels	1386:1395	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	9	27	theme	syndesmophytes	1468:1481	arg1	presence					1456:1463	presence	1456:1463	presence of syndesmophytes (τ = - 0.17, p = 0.001)	1456:1505	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	1	28	theme	glycosaminoglycan	182:198	arg1	content					206:212	the glycosaminoglycan (GAG) content	178:212	the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers	178:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	0	29	theme	proteoglycan	112:123	arg1	depletion					125:133	proteoglycan depletion	112:133	proteoglycan depletion of the lumbar spine	112:153	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	1	30	theme	GAG	358:360	arg1	depletion					362:370	GAG depletion	358:370	GAG depletion	358:370	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	13	31	theme	REGISTRATION	2058:2069	arg1	3980					2071:2074	TRIAL REGISTRATION 3980	2052:2074	TRIAL REGISTRATION 3980 ( https://studienregister.med.uni-duesseldorf.de ).	2052:2126	TRIAL REGISTRATION 3980 ( https://studienregister.med.uni-duesseldorf.de ).					
32943084	4	32	theme	inflammatory	824:835	arg1	activity					837:844	inflammatory activity	824:844	inflammatory activity	824:844	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	10	33	theme	MRI	1593:1595	arg1	scores					1597:1602	MRI scores	1593:1602	MRI scores	1593:1602	No significant correlations were found with BASDAI, inflammation, and fat deposition MRI scores.					
32943084	0	34	theme	Functional	0:9	arg1	imaging					14:20	Functional MR imaging	0:20	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis	0:91	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	11	35	theme	significant	1656:1666	arg1	depletion					1672:1680	significant GAG depletion	1656:1680	significant GAG depletion	1656:1680	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	8	36	dep	lower	1230:1234	arg1	p = 0.001					1301:1309	p = 0.001	1301:1309	p = 0.001	1301:1309	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	4	37	theme	Morphological	769:781	arg1	images					783:788	Morphological images	769:788	Morphological images	769:788	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	2	38	theme	spines	438:443	arg1	METHODS					423:429	METHODS	423:429	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers	423:518	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	7	39	theme	correlation	1151:1161	arg1	analyses					1163:1170	Kendall-Tau correlation analyses	1139:1170	Kendall-Tau correlation analyses	1139:1170	Kendall-Tau correlation analyses were performed.					
32943084	12	40	from	assessment	1968:1977	arg1	spondyloarthritis					2033:2049	axial spondyloarthritis	2027:2049	axial spondyloarthritis	2027:2049	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	13	41	dep	https	2078:2082	arg1	//studienregister.med.uni-duesseldorf.de					2084:2123	//studienregister.med.uni-duesseldorf.de	2084:2123	https://studienregister.med.uni-duesseldorf.de	2078:2123	TRIAL REGISTRATION 3980 ( https://studienregister.med.uni-duesseldorf.de ).					
32943084	3	42	theme	morphological	652:664	arg1	sequences					666:674	high-resolution morphological sequences	636:674	high-resolution morphological sequences	636:674	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	3	43	dep	transfer	731:738	arg1	gagCEST					759:765	gagCEST	759:765	gagCEST	759:765	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	3	43	dep	transfer	731:738	arg1	technique					748:756	imaging technique	740:756	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	13	44	theme	TRIAL	2052:2056	arg1	3980					2071:2074	TRIAL REGISTRATION 3980	2052:2074	TRIAL REGISTRATION 3980 ( https://studienregister.med.uni-duesseldorf.de ).	2052:2126	TRIAL REGISTRATION 3980 ( https://studienregister.med.uni-duesseldorf.de ).					
32943084	2	45	theme	mean	464:467	arg1	patients					454:461	50 AS patients	448:461	50 AS patients (mean age 50 ± 10.5 years)	448:488	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	45	theme	mean	464:467	arg1	50 ± 10.5 years					473:487	mean age 50 ± 10.5 years	464:487	mean age 50 ± 10.5 years	464:487	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	1	46	theme	healthy	301:307	arg1	volunteers					309:318	healthy volunteers	301:318	healthy volunteers	301:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	11	47	theme	patients	1639:1646	arg1	spines					1624:1629	CONCLUSIONS Lumbar spines	1605:1629	CONCLUSIONS Lumbar spines of r-AS patients	1605:1646	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	2	48	dep	spines	438:443	arg1	patients					454:461	50 AS patients	448:461	50 AS patients (mean age 50 ± 10.5 years)	448:488	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	48	dep	spines	438:443	arg1	volunteers					509:518	30 age-matched volunteers	494:518	30 age-matched volunteers	494:518	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	48	dep	spines	438:443	arg1	50 ± 10.5 years					473:487	mean age 50 ± 10.5 years	464:487	mean age 50 ± 10.5 years	464:487	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	0	49	theme	inflammation-radiographic	43:67	arg1	spondyloarthritis					75:91	inflammation-radiographic axial spondyloarthritis	43:91	inflammation-radiographic axial spondyloarthritis	43:91	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	1	50	with	patients	255:262	arg1	spondylitis					280:290	ankylosing spondylitis	269:290	ankylosing spondylitis (AS)	269:295	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	3	51	theme	compositional	684:696	arg1	transfer					731:738	the compositional GAG chemical exchange saturation transfer	680:738	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	9	52	dep	BASFI	1422:1426	arg1	τ = - 0.18					1429:1438	τ = - 0.18	1429:1438	τ = - 0.18	1429:1438	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	12	53	theme	findings	2015:2022	arg1	assessment					1968:1977	the assessment	1964:1977	the assessment of disease-related pathological MRI findings in axial spondyloarthritis	1964:2049	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	13	54	dep	3980	2071:2074	arg1	https					2078:2082	https	2078:2082	https://studienregister.med.uni-duesseldorf.de	2078:2123	TRIAL REGISTRATION 3980 ( https://studienregister.med.uni-duesseldorf.de ).					
32943084	9	55	theme	significant	1323:1333	arg1	correlations					1335:1346	Small, yet significant correlations	1312:1346	Small, yet significant correlations	1312:1346	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	3	56	theme	saturation	720:729	arg1	transfer					731:738	the compositional GAG chemical exchange saturation transfer	680:738	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	11	57	theme	Lumbar	1617:1622	arg1	spines					1624:1629	CONCLUSIONS Lumbar spines	1605:1629	CONCLUSIONS Lumbar spines of r-AS patients	1605:1646	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	1	58	theme	patients	255:262	arg1	IVDs					246:249	IVDs	246:249	IVDs	246:249	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	1	58	theme	patients	255:262	arg1	disks					239:243	lumbar intervertebral disks	217:243	lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers	217:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	12	59	theme	axial	2027:2031	arg1	spondyloarthritis					2033:2049	axial spondyloarthritis	2027:2049	axial spondyloarthritis	2027:2049	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	3	60	theme	chemical	702:709	arg1	transfer					731:738	the compositional GAG chemical exchange saturation transfer	680:738	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	1	61	theme	ankylosing	269:278	arg1	spondylitis					280:290	ankylosing spondylitis	269:290	ankylosing spondylitis (AS)	269:295	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	2	62	theme	conventional	579:590	arg1	CR					605:606	CR	605:606	CR	605:606	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	62	theme	conventional	579:590	arg1	radiographs					592:602	conventional radiographs	579:602	conventional radiographs (CR)	579:607	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	10	63	theme	significant	1511:1521	arg1	correlations					1523:1534	No significant correlations	1508:1534	No significant correlations	1508:1534	No significant correlations were found with BASDAI, inflammation, and fat deposition MRI scores.					
32943084	12	64	theme	pathological	1998:2009	arg1	findings					2015:2022	disease-related pathological MRI findings	1982:2022	disease-related pathological MRI findings	1982:2022	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	3	65	theme	imaging	740:746	arg1	gagCEST					759:765	gagCEST	759:765	gagCEST	759:765	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	3	65	theme	imaging	740:746	arg1	technique					748:756	imaging technique	740:756	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	11	66	theme	inflammation	1787:1798	arg1	markers					1800:1806	increased serological inflammation markers	1765:1806	increased serological inflammation markers	1765:1806	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	0	67	theme	spine	149:153	arg1	depletion					125:133	proteoglycan depletion	112:133	proteoglycan depletion of the lumbar spine	112:153	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	2	68	theme	resonance	551:559	arg1	MRI					570:572	MRI	570:572	MRI	570:572	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	68	theme	resonance	551:559	arg1	imaging					561:567	3-T magnetic resonance imaging	538:567	3-T magnetic resonance imaging (MRI)	538:573	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	9	69	dep	values	1375:1380	arg1	p = 0.007					1410:1418	p = 0.007	1410:1418	p = 0.007	1410:1418	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	6	70	theme	linear	1119:1124	arg1	model					1132:1136	a linear mixed model	1117:1136	a linear mixed model	1117:1136	GagCEST values of both groups were compared using a linear mixed model.					
32943084	10	71	theme	fat	1578:1580	arg1	deposition					1582:1591	fat deposition	1578:1591	fat deposition	1578:1591	No significant correlations were found with BASDAI, inflammation, and fat deposition MRI scores.					
32943084	11	72	theme	increased	1765:1773	arg1	markers					1800:1806	increased serological inflammation markers	1765:1806	increased serological inflammation markers	1765:1806	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	1	73	theme	intervertebral	224:237	arg1	IVDs					246:249	IVDs	246:249	IVDs	246:249	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	1	73	theme	intervertebral	224:237	arg1	disks					239:243	lumbar intervertebral disks	217:243	lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers	217:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	2	74	theme	3-T	538:540	arg1	MRI					570:572	MRI	570:572	MRI	570:572	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	74	theme	3-T	538:540	arg1	imaging					561:567	3-T magnetic resonance imaging	538:567	3-T magnetic resonance imaging (MRI)	538:573	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	1	75	theme	features	413:420	arg1	association					343:353	the association	339:353	the association of GAG depletion and disease-related clinical and imaging features	339:420	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	12	76	contain	have	1927:1930	arg1	imaging					1915:1921	gagCEST imaging	1907:1921	gagCEST imaging	1907:1921	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	12	76	contain	have	1927:1930	arg2	role					1956:1959	an adjunct confirmatory role	1932:1959	an adjunct confirmatory role	1932:1959	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	5	77	theme	serological	925:935	arg1	measures					937:944	Clinical and serological measures	912:944	Clinical and serological measures	912:944	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	12	78	theme	adjunct	1935:1941	arg1	role					1956:1959	an adjunct confirmatory role	1932:1959	an adjunct confirmatory role	1932:1959	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	8	79	theme	healthy	1268:1274	arg1	volunteers					1276:1285	healthy volunteers	1268:1285	healthy volunteers (2.4 ± 1.8%)	1268:1298	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	8	79	theme	healthy	1268:1274	arg1	%					1297:1297	2.4 ± 1.8%	1288:1297	2.4 ± 1.8%	1288:1297	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	5	80	theme	protein	1045:1051	arg1	levels					1059:1064	C-reactive protein (CRP) levels	1034:1064	C-reactive protein (CRP) levels	1034:1064	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	5	80	theme	protein	1045:1051	arg1	Bath					959:962	the Bath	955:962	the Bath	955:962	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	4	81	from	activity	837:844	arg1	CR					908:909	CR	908:909	CR	908:909	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	4	82	from	changes	897:903	arg1	CR					908:909	CR	908:909	CR	908:909	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	12	83	theme	gagCEST	1907:1913	arg1	imaging					1915:1921	gagCEST imaging	1907:1921	gagCEST imaging	1907:1921	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	5	84	theme	Clinical	912:919	arg1	measures					937:944	Clinical and serological measures	912:944	Clinical and serological measures	912:944	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	11	85	theme	functional	1738:1747	arg1	disability					1749:1758	functional disability	1738:1758	functional disability	1738:1758	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	1	86	theme	depletion	362:370	arg1	association					343:353	the association	339:353	the association of GAG depletion and disease-related clinical and imaging features	339:420	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	6	87	theme	GagCEST	1067:1073	arg1	values					1075:1080	GagCEST values	1067:1080	GagCEST values of both groups	1067:1095	GagCEST values of both groups were compared using a linear mixed model.					
32943084	0	88	theme	MR	11:12	arg1	imaging					14:20	Functional MR imaging	0:20	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis	0:91	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	1	89	theme	disease-related	376:390	arg1	features					413:420	disease-related clinical and imaging features	376:420	disease-related clinical and imaging features	376:420	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	4	90	from	degeneration	868:879	arg1	CR					908:909	CR	908:909	CR	908:909	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	2	91	theme	AS	451:452	arg1	patients					454:461	50 AS patients	448:461	50 AS patients (mean age 50 ± 10.5 years)	448:488	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	91	theme	AS	451:452	arg1	50 ± 10.5 years					473:487	mean age 50 ± 10.5 years	464:487	mean age 50 ± 10.5 years	464:487	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	7	92	theme	Kendall-Tau	1139:1149	arg1	analyses					1163:1170	Kendall-Tau correlation analyses	1139:1170	Kendall-Tau correlation analyses	1139:1170	Kendall-Tau correlation analyses were performed.					
32943084	9	93	theme	Small	1312:1316	arg1	correlations					1335:1346	Small, yet significant correlations	1312:1346	Small, yet significant correlations	1312:1346	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	12	94	theme	cartilage	1862:1870	arg1	role					1850:1853	a pathophysiological role	1829:1853	a pathophysiological role of the cartilage in AS	1829:1876	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	5	95	theme	Disease	967:973	arg1	BASDAI					985:990	BASDAI	985:990	BASDAI	985:990	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	5	95	theme	Disease	967:973	arg1	Activity					975:982	Disease Activity	967:982	Disease Activity (BASDAI)	967:991	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	3	96	theme	high-resolution	636:650	arg1	sequences					666:674	high-resolution morphological sequences	636:674	high-resolution morphological sequences	636:674	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32943084	9	97	dep	syndesmophytes	1468:1481	arg1	p = 0.001					1496:1504	p = 0.001	1496:1504	p = 0.001	1496:1504	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	2	98	theme	Lumbar	431:436	arg1	spines					438:443	Lumbar spines	431:443	Lumbar spines	431:443	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	5	99	theme	BASFI	1015:1019	arg1	Bath					959:962	the Bath	955:962	the Bath	955:962	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	5	99	theme	BASFI	1015:1019	arg1	Indices					1022:1028	Function (BASFI) Indices	1005:1028	Function (BASFI) Indices	1005:1028	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	2	100	theme	age	469:471	arg1	patients					454:461	50 AS patients	448:461	50 AS patients (mean age 50 ± 10.5 years)	448:488	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	2	100	theme	age	469:471	arg1	50 ± 10.5 years					473:487	mean age 50 ± 10.5 years	464:487	mean age 50 ± 10.5 years	464:487	METHODS Lumbar spines of 50 AS patients (mean age 50 ± 10.5 years) and 30 age-matched volunteers were studied with 3-T magnetic resonance imaging (MRI) and conventional radiographs (CR).					
32943084	11	101	theme	r-AS	1634:1637	arg1	patients					1639:1646	r-AS patients	1634:1646	r-AS patients	1634:1646	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	0	102	theme	axial	69:73	arg1	spondyloarthritis					75:91	inflammation-radiographic axial spondyloarthritis	43:91	inflammation-radiographic axial spondyloarthritis	43:91	Functional MR imaging beyond structure and inflammation-radiographic axial spondyloarthritis is associated with proteoglycan depletion of the lumbar spine.					
32943084	1	103	theme	volunteers	309:318	arg1	IVDs					246:249	IVDs	246:249	IVDs	246:249	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	1	103	theme	volunteers	309:318	arg1	disks					239:243	lumbar intervertebral disks	217:243	lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers	217:318	BACKGROUND To compare the glycosaminoglycan (GAG) content of lumbar intervertebral disks (IVDs) of patients with ankylosing spondylitis (AS) and healthy volunteers and to investigate the association of GAG depletion and disease-related clinical and imaging features.					
32943084	12	104	theme	pathophysiological	1831:1848	arg1	role					1850:1853	a pathophysiological role	1829:1853	a pathophysiological role of the cartilage in AS	1829:1876	Beyond establishing a pathophysiological role of the cartilage in AS, these findings suggest that gagCEST imaging may have an adjunct confirmatory role in the assessment of disease-related pathological MRI findings in axial spondyloarthritis.					
32943084	8	105	dep	RESULTS	1188:1194	arg1	lower					1230:1234	lower	1230:1234	lower	1230:1234	RESULTS GagCEST values were significantly lower in AS patients (2.0 ± 1.7%) vs. healthy volunteers (2.4 ± 1.8%), p = 0.001.					
32943084	4	106	theme	fat	847:849	arg1	deposition					851:860	fat deposition	847:860	fat deposition	847:860	Morphological images were analyzed by three raters for inflammatory activity, fat deposition, disk degeneration, and structural changes on CR.					
32943084	11	107	theme	GAG	1668:1670	arg1	depletion					1672:1680	significant GAG depletion	1656:1680	significant GAG depletion	1656:1680	CONCLUSIONS Lumbar spines of r-AS patients undergo significant GAG depletion, independently associated with syndesmophyte formation, functional disability, and increased serological inflammation markers.					
32943084	9	108	theme	gagCEST	1367:1373	arg1	values					1375:1380	gagCEST values	1367:1380	gagCEST values	1367:1380	Small, yet significant correlations were found between gagCEST values and CRP levels (τ = - 0.14, p = 0.007), BASFI (τ = - 0.18, p < 0.001) and presence of syndesmophytes (τ = - 0.17, p = 0.001).					
32943084	5	109	theme	Function	1005:1012	arg1	Bath					959:962	the Bath	955:962	the Bath	955:962	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	5	109	theme	Function	1005:1012	arg1	Indices					1022:1028	Function (BASFI) Indices	1005:1028	Function (BASFI) Indices	1005:1028	Clinical and serological measures included the Bath AS Disease Activity (BASDAI) and Bath AS Function (BASFI) Indices and C-reactive protein (CRP) levels.					
32943084	6	110	theme	groups	1090:1095	arg1	values					1075:1080	GagCEST values	1067:1080	GagCEST values of both groups	1067:1095	GagCEST values of both groups were compared using a linear mixed model.					
32943084	3	111	theme	exchange	711:718	arg1	transfer					731:738	the compositional GAG chemical exchange saturation transfer	680:738	the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST)	680:766	The MRI protocol included high-resolution morphological sequences and the compositional GAG chemical exchange saturation transfer imaging technique (gagCEST).					
32088749	0	0	theme	Lactobacilli	86:97	arg1	Diversity					73:81	Diversity	73:81	Diversity	73:81	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	0	0	theme	Lactobacilli	86:97	arg1	Population					58:67	Population	58:67	Population	58:67	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	4	1	theme	lactobacilli	1029:1040	arg1	unique					1053:1058	unique	1053:1058	unique	1053:1058	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	4	1	theme	lactobacilli	1029:1040	arg1	composition					1014:1024	The species composition	1002:1024	The species composition of lactobacilli	1002:1040	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	1	2	from	present	189:195	arg1	feces					200:204	feces	200:204	feces of infants	200:215	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	3	3	theme	prebiotic-containing	893:912	arg1	diets					914:918	all infant receiving prebiotic-containing diets	872:918	all infant receiving prebiotic-containing diets	872:918	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	3	4	theme	fecal	829:833	arg1	species					848:854	fecal lactobacilli species	829:854	fecal lactobacilli species	829:854	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	1	5	theme	both	393:396	arg1	combination					378:388	combination	378:388	combination of both	378:396	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	1	5	theme	both	393:396	arg1	infant formulae					358:372	inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae	302:372	inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae	302:372	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	3	6	theme	lactobacilli	835:846	arg1	species					848:854	fecal lactobacilli species	829:854	fecal lactobacilli species	829:854	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	2	7	located	detected	719:726	arg1	breast-fed					747:756	breast-fed	747:756	breast-fed	747:756	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	7	located	detected	719:726	arg2	those					713:717	those	713:717	those	713:717	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	0	8	from	Impacts	0:6	arg1	Diversity					73:81	Diversity	73:81	Diversity	73:81	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	0	8	from	Impacts	0:6	arg1	Population					58:67	Population	58:67	Population	58:67	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	2	9	theme	C2-C	634:637	arg1	infant					639:644	C2-C infant	634:644	C2-C infant	634:644	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	9	theme	C2-C	634:637	arg1	inulin-GOS					622:631	inulin-GOS	622:631	inulin-GOS (C2-C infant)	622:645	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	10	contain	containing	579:588	arg2	inulin-GOS					622:631	inulin-GOS	622:631	inulin-GOS (C2-C infant)	622:645	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	10	contain	containing	579:588	arg1	formula					571:577	a formula	569:577	a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant)	569:645	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	10	contain	containing	579:588	arg2	infant					611:616	C1-A infant	606:616	C1-A infant	606:616	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	10	contain	containing	579:588	arg2	FOS-GOS					597:603	FOS-GOS	597:603	FOS-GOS (C1-A infant)	597:617	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	10	contain	containing	579:588	arg2	infant					639:644	C2-C infant	634:644	C2-C infant	634:644	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	6	11	from	supplementation	1330:1344	arg1	formulae					1356:1363	infant formulae	1349:1363	infant formulae	1349:1363	Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.					
32088749	3	12	dep	observed	860:867	arg1	whereas					921:927	whereas	921:927	whereas	921:927	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	2	13	dep	breast-fed	747:756	arg1	infants					773:779	B1-D and B2-E infants	759:779	B1-D and B2-E infants	759:779	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	13	dep	breast-fed	747:756	arg1	P < 0.05					783:790	P < 0.05	783:790	P < 0.05	783:790	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	0	14	theme	Healthy	119:125	arg1	Infants					127:133	Thai Healthy Infants	114:133	Thai Healthy Infants	114:133	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	2	15	theme	cell	476:479	arg1	counts					481:486	maximum total cell counts	462:486	maximum total cell counts	462:486	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	16	theme	B2-E	768:771	arg1	infants					773:779	B1-D and B2-E infants	759:779	B1-D and B2-E infants	759:779	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	1	17	theme	lactobacilli	176:187	arg1	diversity					163:171	diversity	163:171	diversity	163:171	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	1	17	theme	lactobacilli	176:187	arg1	abundance					149:157	relative abundance	140:157	relative abundance	140:157	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	0	18	theme	Thai	114:117	arg1	Infants					127:133	Thai Healthy Infants	114:133	Thai Healthy Infants	114:133	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	5	19	theme	transferred	1259:1269	arg1	infants					1295:1301	indigenous lactobacilli vertically transferred and continuously seeded infants'	1224:1302	indigenous lactobacilli vertically transferred and continuously seeded infants' gut	1224:1306	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	5	20	theme	seeded	1288:1293	arg1	infants					1295:1301	indigenous lactobacilli vertically transferred and continuously seeded infants'	1224:1302	indigenous lactobacilli vertically transferred and continuously seeded infants' gut	1224:1306	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	1	21	theme	present	189:195	arg1	lactobacilli					176:187	lactobacilli	176:187	lactobacilli present in feces of infants	176:215	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	2	22	theme	B1-D	759:762	arg1	infants					773:779	B1-D and B2-E infants	759:779	B1-D and B2-E infants	759:779	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	6	23	theme	Meanwhile	1309:1317	arg1	supplementation					1330:1344	Meanwhile, prebiotic supplementation	1309:1344	Meanwhile, prebiotic supplementation in infant formulae	1309:1363	Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.					
32088749	2	24	theme	F2-H	694:697	arg1	infants					699:705	F2-H infants	694:705	F2-H infants	694:705	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	0	25	theme	Diets	34:38	arg1	Impacts					0:6	Impacts	0:6	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli	0:97	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	5	26	theme	indigenous	1224:1233	arg1	infants					1295:1301	indigenous lactobacilli vertically transferred and continuously seeded infants'	1224:1302	indigenous lactobacilli vertically transferred and continuously seeded infants' gut	1224:1306	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	2	27	theme	C1-A	606:609	arg1	FOS-GOS					597:603	FOS-GOS	597:603	FOS-GOS (C1-A infant)	597:617	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	27	theme	C1-A	606:609	arg1	infant					611:616	C1-A infant	606:616	C1-A infant	606:616	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	28	theme	fed	675:677	arg1	ones					679:682	the exclusively formula fed ones	651:682	the exclusively formula fed ones (F1-F and F2-H infants)	651:706	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	1	29	dep	abundance	149:157	arg1	The					136:138	The	136:138	The	136:138	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	0	30	theme	Prebiotic-Supplemented	11:32	arg1	Diets					34:38	Prebiotic-Supplemented Diets	11:38	Prebiotic-Supplemented Diets	11:38	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	5	31	theme	lactobacilli	1235:1246	arg1	infants					1295:1301	indigenous lactobacilli vertically transferred and continuously seeded infants'	1224:1302	indigenous lactobacilli vertically transferred and continuously seeded infants' gut	1224:1306	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	6	32	theme	lactobacilli	1419:1430	arg1	colonization					1403:1414	the successful colonization	1388:1414	the successful colonization of lactobacilli	1388:1430	Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.					
32088749	1	33	attach	present	189:195	arg2	lactobacilli					176:187	lactobacilli	176:187	lactobacilli present in feces of infants	176:215	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	1	33	attach	present	189:195	arg1	feces					200:204	feces	200:204	feces of infants	200:215	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	1	34	theme	infants	209:215	arg1	feces					200:204	feces	200:204	feces of infants	200:215	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	0	35	theme	Breastmilk	44:53	arg1	Impacts					0:6	Impacts	0:6	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli	0:97	Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants.					
32088749	4	36	from	groups	1103:1108	arg1	unique					1053:1058	unique	1053:1058	unique	1053:1058	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	4	36	from	groups	1103:1108	arg1	composition					1014:1024	The species composition	1002:1024	The species composition of lactobacilli	1002:1040	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	4	37	theme	formula-fed	1163:1173	arg1	infants					1175:1181	the exclusively formula-fed infants	1147:1181	the exclusively formula-fed infants	1147:1181	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	2	38	theme	total	470:474	arg1	counts					481:486	maximum total cell counts	462:486	maximum total cell counts	462:486	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	3	39	theme	receiving	883:891	arg1	diets					914:918	all infant receiving prebiotic-containing diets	872:918	all infant receiving prebiotic-containing diets	872:918	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	5	40	theme	gut	1304:1306	arg1	Breastmilk					1184:1193	Breastmilk	1184:1193	Breastmilk	1184:1193	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	5	40	theme	gut	1304:1306	arg1	source					1214:1219	a great source	1206:1219	a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut	1206:1306	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	2	41	theme	maximum	462:468	arg1	counts					481:486	maximum total cell counts	462:486	maximum total cell counts	462:486	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	1	42	theme	-supplemented	344:356	arg1	infant formulae					358:372	inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae	302:372	inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae	302:372	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	6	43	theme	successful	1392:1401	arg1	colonization					1403:1414	the successful colonization	1388:1414	the successful colonization of lactobacilli	1388:1430	Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.					
32088749	6	44	dep	Meanwhile	1309:1317	arg1	prebiotic					1320:1328	prebiotic	1320:1328	prebiotic	1320:1328	Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.					
32088749	5	45	theme	great	1208:1212	arg1	Breastmilk					1184:1193	Breastmilk	1184:1193	Breastmilk	1184:1193	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	5	45	theme	great	1208:1212	arg1	source					1214:1219	a great source	1206:1219	a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut	1206:1306	Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants' gut.					
32088749	3	46	theme	breast-fed	982:991	arg1	infants					993:999	exclusively breast-fed infants	970:999	exclusively breast-fed infants	970:999	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	6	47	theme	infant	1349:1354	arg1	formulae					1356:1363	infant formulae	1349:1363	infant formulae	1349:1363	Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.					
32088749	4	48	theme	infants	1113:1119	arg1	groups					1103:1108	both groups	1098:1108	both groups of infants receiving breastmilk than the exclusively formula-fed infants	1098:1181	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	4	48	theme	infants	1113:1119	arg1	infants					1113:1119	infants	1113:1119	infants receiving breastmilk than the exclusively formula-fed infants	1113:1181	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	3	49	theme	greatest	798:805	arg1	abundance					816:824	The greatest relative abundance	794:824	The greatest relative abundance of fecal lactobacilli species	794:854	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	3	50	located	observed	860:867	arg1	diets					914:918	all infant receiving prebiotic-containing diets	872:918	all infant receiving prebiotic-containing diets	872:918	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	3	50	located	observed	860:867	arg2	abundance					816:824	The greatest relative abundance	794:824	The greatest relative abundance of fecal lactobacilli species	794:854	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	1	51	theme	relative	140:147	arg1	abundance					149:157	relative abundance	140:157	relative abundance	140:157	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	3	52	theme	species	848:854	arg1	abundance					816:824	The greatest relative abundance	794:824	The greatest relative abundance of fecal lactobacilli species	794:854	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	3	53	theme	relative	807:814	arg1	abundance					816:824	The greatest relative abundance	794:824	The greatest relative abundance of fecal lactobacilli species	794:854	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	3	54	theme	infant	876:881	arg1	diets					914:918	all infant receiving prebiotic-containing diets	872:918	all infant receiving prebiotic-containing diets	872:918	The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants.					
32088749	1	55	from	feces	200:204	arg1	present					189:195	present	189:195	present	189:195	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	1	56	theme	fructooligosaccharide-galactooligosaccharide	241:284	arg1	FOS-GOS					287:293	fructooligosaccharide-galactooligosaccharide (FOS-GOS)-	241:295	fructooligosaccharide-galactooligosaccharide (FOS-GOS)-	241:295	The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared.					
32088749	4	57	theme	species	1006:1012	arg1	unique					1053:1058	unique	1053:1058	unique	1053:1058	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	4	57	theme	species	1006:1012	arg1	composition					1014:1024	The species composition	1002:1024	The species composition of lactobacilli	1002:1040	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	2	58	theme	Fecal	413:417	arg1	lactobacilli					419:430	Fecal lactobacilli	413:430	Fecal lactobacilli	413:430	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	59	dep	ones	679:682	arg1	F1-F					685:688	F1-F	685:688	F1-F	685:688	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	2	59	dep	ones	679:682	arg1	infants					699:705	F2-H infants	694:705	F2-H infants	694:705	Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05).					
32088749	4	60	from	unique	1053:1058	arg1	groups					1103:1108	both groups	1098:1108	both groups of infants receiving breastmilk than the exclusively formula-fed infants	1098:1181	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
32088749	4	60	from	unique	1053:1058	arg1	infants					1113:1119	infants	1113:1119	infants receiving breastmilk than the exclusively formula-fed infants	1113:1181	The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants.					
34071337	0	0	theme	Reinforcing	92:102	arg1	Properties					104:113	Antibacterial and Reinforcing Properties	74:113	Antibacterial and Reinforcing Properties	74:113	Facile Synthesis and Characterization of Palm CNF-ZnO Nanocomposites with Antibacterial and Reinforcing Properties.					
34071337	5	1	dep	34	894:895	arg1	to					891:892	to	891:892	to	891:892	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	8	2	theme	inhibitory	1122:1131	arg1	activities					1133:1142	potent inhibitory activities	1115:1142	potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1115:1272	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	6	3	theme	ZnO	958:960	arg1	particles					962:970	ultrafine ZnO particles	948:970	ultrafine ZnO particles	948:970	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	3	4	theme	facile	560:565	arg1	route					592:596	a facile and efficient ultrasound route	558:596	a facile and efficient ultrasound route	558:596	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	4	5	theme	mechanical	651:660	arg1	performances					676:687	the antibacterial and mechanical reinforcement performances	629:687	the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite	629:731	We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite.					
34071337	8	6	dep	Gram-positive	1152:1164	arg1	MRSA					1212:1215	MRSA	1212:1215	MRSA	1212:1215	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	8	6	dep	Gram-positive	1152:1164	arg1	aureus					1204:1209	Staphylococcus aureus	1189:1209	methicillin-resistant Staphylococcus aureus (MRSA)	1167:1216	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	1	7	attach	isolated	143:150	arg2	nanofibers					126:135	Cellulose nanofibers	116:135	Cellulose nanofibers (CNF) isolated from plant biomass	116:169	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	1	7	attach	isolated	143:150	arg1	biomass					163:169	plant biomass	157:169	plant biomass	157:169	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	1	7	attach	isolated	143:150	arg2	CNF					138:140	CNF	138:140	CNF	138:140	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	5	8	theme	electron	747:754	arg1	microscopy					756:765	electron microscopy	747:765	our electron microscopy analyses	743:774	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	7	9	theme	thermal	1054:1060	arg1	stability					1062:1070	improved thermal stability	1045:1070	improved thermal stability	1045:1070	The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF.					
34071337	8	10	theme	potent	1115:1120	arg1	activities					1133:1142	potent inhibitory activities	1115:1142	potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1115:1272	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	10	11	theme	CNF-ZnO	1548:1554	arg1	nanocomposites					1556:1569	ultrasonically-synthesized palm CNF-ZnO nanocomposites	1516:1569	ultrasonically-synthesized palm CNF-ZnO nanocomposites	1516:1569	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	6	12	theme	uniform	979:985	arg1	distribution					992:1003	a uniform size distribution	977:1003	a uniform size distribution	977:1003	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	5	13	theme	microscopy	756:765	arg1	analyses					767:774	our electron microscopy analyses	743:774	our electron microscopy analyses	743:774	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	6	14	theme	pH	901:902	arg1	levels					904:909	pH levels	901:909	pH levels between 8-10	901:922	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	2	15	theme	preparation	329:339	arg1	methods					341:347	the time-consuming preparation methods	310:347	the time-consuming preparation methods	310:347	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	9	16	theme	rubber	1304:1309	arg1	film					1334:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	2	17	theme	time-consuming	314:327	arg1	methods					341:347	the time-consuming preparation methods	310:347	the time-consuming preparation methods	310:347	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	4	18	theme	reinforcement	662:674	arg1	performances					676:687	the antibacterial and mechanical reinforcement performances	629:687	the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite	629:731	We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite.					
34071337	5	19	theme	flake-like	830:839	arg1	morphology					841:850	a flake-like morphology	828:850	a flake-like morphology with particle sizes ranging between 21 to 34 nm	828:898	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	10	20	theme	study	1497:1501	arg1	findings					1480:1487	The findings	1476:1487	The findings of this study	1476:1501	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	10	21	theme	potential	1582:1590	arg1	applications					1592:1603	potential applications	1582:1603	potential applications	1582:1603	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	7	22	theme	improved	1045:1052	arg1	stability					1062:1070	improved thermal stability	1045:1070	improved thermal stability	1045:1070	The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF.					
34071337	10	23	theme	palm	1543:1546	arg1	nanocomposites					1556:1569	ultrasonically-synthesized palm CNF-ZnO nanocomposites	1516:1569	ultrasonically-synthesized palm CNF-ZnO nanocomposites	1516:1569	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	6	24	with	formation	935:943	arg1	distribution					992:1003	a uniform size distribution	977:1003	a uniform size distribution	977:1003	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	3	25	theme	multifunctional	448:462	arg1	nanocomposite					489:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite	446:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties	446:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	6	26	theme	particles	962:970	arg1	formation					935:943	the formation	931:943	the formation of ultrafine ZnO particles with a uniform size distribution	931:1003	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	0	27	theme	Palm	41:44	arg1	CNF-ZnO					46:52	Palm CNF-ZnO	41:52	Palm CNF-ZnO	41:52	Facile Synthesis and Characterization of Palm CNF-ZnO Nanocomposites with Antibacterial and Reinforcing Properties.					
34071337	7	28	theme	CNF-ZnO	1020:1026	arg1	composite					1028:1036	The resultant CNF-ZnO composite	1006:1036	The resultant CNF-ZnO composite	1006:1036	The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF.					
34071337	1	29	theme	considerable	186:197	arg1	interests					199:207	considerable interests	186:207	considerable interests	186:207	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	5	30	contain	had	824:826	arg1	ZnO					781:783	the ZnO	777:783	the ZnO deposited onto the nanofibrous network	777:822	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	5	30	contain	had	824:826	arg2	morphology					841:850	a flake-like morphology	828:850	a flake-like morphology with particle sizes ranging between 21 to 34 nm	828:898	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	9	31	from	improvement	1417:1427	arg1	strength					1440:1447	tensile strength	1432:1447	tensile strength	1432:1447	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	10	32	theme	strength	1660:1667	arg1	composites					1676:1685	high strength rubber composites	1655:1685	high strength rubber composites	1655:1685	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	0	33	theme	Facile	0:5	arg1	Synthesis					7:15	Facile Synthesis	0:15	Facile Synthesis	0:15	Facile Synthesis and Characterization of Palm CNF-ZnO Nanocomposites with Antibacterial and Reinforcing Properties.					
34071337	9	34	theme	natural	1296:1302	arg1	film					1334:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	10	35	theme	biomedical	1612:1621	arg1	field					1623:1627	the biomedical field	1608:1627	the biomedical field	1608:1627	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	9	36	theme	composite	1324:1332	arg1	film					1334:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	3	37	theme	CNF-ZnO	480:486	arg1	nanocomposite					489:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite	446:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties	446:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	10	38	theme	ultrasonically-synthesized	1516:1541	arg1	nanocomposites					1556:1569	ultrasonically-synthesized palm CNF-ZnO nanocomposites	1516:1569	ultrasonically-synthesized palm CNF-ZnO nanocomposites	1516:1569	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	7	39	theme	resultant	1010:1018	arg1	composite					1028:1036	The resultant CNF-ZnO composite	1006:1036	The resultant CNF-ZnO composite	1006:1036	The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF.					
34071337	1	40	theme	Cellulose	116:124	arg1	CNF					138:140	CNF	138:140	CNF	138:140	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	1	40	theme	Cellulose	116:124	arg1	nanofibers					126:135	Cellulose nanofibers	116:135	Cellulose nanofibers (CNF) isolated from plant biomass	116:169	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	9	41	theme	CNF-ZnO-reinforced	1277:1294	arg1	film					1334:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	1	42	theme	polymer	212:218	arg1	engineering					220:230	polymer engineering	212:230	polymer engineering	212:230	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	4	43	theme	antibacterial	633:645	arg1	performances					676:687	the antibacterial and mechanical reinforcement performances	629:687	the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite	629:731	We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite.					
34071337	6	44	theme	ultrafine	948:956	arg1	particles					962:970	ultrafine ZnO particles	948:970	ultrafine ZnO particles	948:970	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	10	45	theme	high	1655:1658	arg1	composites					1676:1685	high strength rubber composites	1655:1685	high strength rubber composites	1655:1685	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	4	46	theme	nanocomposite	719:731	arg1	performances					676:687	the antibacterial and mechanical reinforcement performances	629:687	the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite	629:731	We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite.					
34071337	3	47	theme	CNF-zinc	464:471	arg1	nanocomposite					489:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite	446:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties	446:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	5	48	theme	nanofibrous	804:814	arg1	network					816:822	the nanofibrous network	800:822	the nanofibrous network	800:822	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	2	49	theme	CNF-based	265:273	arg1	nanocomposites					275:288	CNF-based nanocomposites	265:288	CNF-based nanocomposites	265:288	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	9	50	theme	tensile	1432:1438	arg1	strength					1440:1447	tensile strength	1432:1447	tensile strength	1432:1447	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	3	51	theme	oxide	473:477	arg1	nanocomposite					489:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite	446:501	a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties	446:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	9	52	dep	42	1413:1414	arg1	to					1410:1411	to	1410:1411	to	1410:1411	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	5	53	with	morphology	841:850	arg1	sizes					866:870	particle sizes	857:870	particle sizes ranging between 21 to 34 nm	857:898	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	8	54	theme	Gram-positive	1152:1164	arg1	bacteria					1265:1272	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	3	55	theme	dual	508:511	arg1	properties					543:552	dual antibacterial and reinforcing properties	508:552	dual antibacterial and reinforcing properties	508:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	9	56	dep	%	1415:1415	arg1	42					1413:1414	42	1413:1414	42	1413:1414	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	8	57	theme	typhi	1248:1252	arg1	bacteria					1265:1272	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	0	58	theme	CNF-ZnO	46:52	arg1	Characterization					21:36	Characterization	21:36	Characterization	21:36	Facile Synthesis and Characterization of Palm CNF-ZnO Nanocomposites with Antibacterial and Reinforcing Properties.					
34071337	0	58	theme	CNF-ZnO	46:52	arg1	Synthesis					7:15	Facile Synthesis	0:15	Facile Synthesis	0:15	Facile Synthesis and Characterization of Palm CNF-ZnO Nanocomposites with Antibacterial and Reinforcing Properties.					
34071337	3	59	theme	reinforcing	531:541	arg1	properties					543:552	dual antibacterial and reinforcing properties	508:552	dual antibacterial and reinforcing properties	508:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	6	60	theme	size	987:990	arg1	distribution					992:1003	a uniform size distribution	977:1003	a uniform size distribution	977:1003	pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution.					
34071337	1	61	theme	plant	157:161	arg1	biomass					163:169	plant biomass	157:169	plant biomass	157:169	Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering.					
34071337	2	62	theme	functionalities	377:391	arg1	lack					353:356	lack	353:356	lack of desired surface functionalities	353:391	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	2	62	theme	functionalities	377:391	arg1	methods					341:347	the time-consuming preparation methods	310:347	the time-consuming preparation methods	310:347	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	9	63	dep	improvement	1417:1427	arg1	%					1415:1415	%	1415:1415	%	1415:1415	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	9	64	theme	neat	1467:1470	arg1	NR					1472:1473	the neat NR	1463:1473	the neat NR	1463:1473	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	8	65	theme	Salmonella	1237:1246	arg1	typhi					1248:1252	Salmonella typhi	1237:1252	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	8	65	theme	Salmonella	1237:1246	arg1	typhi					1258:1262	typhi	1258:1262	typhi	1258:1262	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	9	66	theme	NR/CNF-ZnO	1312:1321	arg1	film					1334:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film	1275:1337	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	8	67	theme	Gram-negative	1223:1235	arg1	bacteria					1265:1272	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria	1152:1272	The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria.					
34071337	2	68	theme	surface	369:375	arg1	functionalities					377:391	desired surface functionalities	361:391	desired surface functionalities	361:391	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	3	69	with	nanocomposite	489:501	arg1	properties					543:552	dual antibacterial and reinforcing properties	508:552	dual antibacterial and reinforcing properties	508:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	7	70	theme	pure	1084:1087	arg1	CNF					1089:1091	pure CNF	1084:1091	pure CNF	1084:1091	The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF.					
34071337	2	71	theme	desired	361:367	arg1	functionalities					377:391	desired surface functionalities	361:391	desired surface functionalities	361:391	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	2	72	attach	linked	300:305	arg2	limitations					237:247	The limitations	233:247	The limitations associated with CNF-based nanocomposites	233:288	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	2	72	attach	linked	300:305	arg1	lack					353:356	lack	353:356	lack of desired surface functionalities	353:391	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	2	72	attach	linked	300:305	arg1	methods					341:347	the time-consuming preparation methods	310:347	the time-consuming preparation methods	310:347	The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities.					
34071337	0	73	theme	Antibacterial	74:86	arg1	Properties					104:113	Antibacterial and Reinforcing Properties	74:113	Antibacterial and Reinforcing Properties	74:113	Facile Synthesis and Characterization of Palm CNF-ZnO Nanocomposites with Antibacterial and Reinforcing Properties.					
34071337	3	74	theme	antibacterial	513:525	arg1	properties					543:552	dual antibacterial and reinforcing properties	508:552	dual antibacterial and reinforcing properties	508:552	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	3	75	theme	efficient	571:579	arg1	route					592:596	a facile and efficient ultrasound route	558:596	a facile and efficient ultrasound route	558:596	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
34071337	9	76	theme	latex	1363:1367	arg1	mixing					1369:1374	latex mixing	1363:1374	latex mixing	1363:1374	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	4	77	theme	induced	711:717	arg1	nanocomposite					719:731	our ultrasonically induced nanocomposite	692:731	our ultrasonically induced nanocomposite	692:731	We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite.					
34071337	5	78	theme	particle	857:864	arg1	sizes					866:870	particle sizes	857:870	particle sizes ranging between 21 to 34 nm	857:898	Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm.					
34071337	9	79	theme	casting	1380:1386	arg1	methods					1388:1394	casting methods	1380:1394	casting methods	1380:1394	A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR.					
34071337	10	80	theme	composites	1676:1685	arg1	development					1640:1650	the development	1636:1650	the development of high strength rubber composites	1636:1685	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	10	81	theme	rubber	1669:1674	arg1	composites					1676:1685	high strength rubber composites	1655:1685	high strength rubber composites	1655:1685	The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.					
34071337	3	82	theme	ultrasound	581:590	arg1	route					592:596	a facile and efficient ultrasound route	558:596	a facile and efficient ultrasound route	558:596	Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route.					
32179117	3	0	dep	magnetite	532:540	arg1	nanoparticles					592:604	nanoparticles	592:604	nanoparticles	592:604	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	7	1	from	nanocomposites	1335:1348	arg1	release					1309:1315	cisP loading and release	1292:1315	release	1309:1315	The UV-Vis spectroscopy was used to study cisP loading and release from the prepared nanocomposites.					
32179117	7	1	from	nanocomposites	1335:1348	arg1	loading					1297:1303	cisP loading and release	1292:1315	loading	1297:1303	The UV-Vis spectroscopy was used to study cisP loading and release from the prepared nanocomposites.					
32179117	9	2	theme	releasing	1492:1500	arg1	potentiality					1502:1513	the releasing potentiality	1488:1513	While the releasing potentiality for CS/M composite	1482:1532	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	9	2	theme	releasing	1492:1500	arg1	highest					1542:1548	highest	1542:1548	highest	1542:1548	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	3	3	theme	release	474:480	arg1	potentiality					482:493	release potentiality	474:493	release potentiality	474:493	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	6	4	theme	chitosan-functionalized	1178:1200	arg1	nanocomposite					1202:1214	a selected chitosan-functionalized nanocomposite	1167:1214	a selected chitosan-functionalized nanocomposite	1167:1214	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	dioxide					668:674	silicon dioxide	660:674	silicon dioxide	660:674	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	CS/M/S/GO					697:705	CS/M/S/GO	697:705	CS/M/S/GO	697:705	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	dioxide					789:795	chitosan-coated silicon dioxide	765:795	chitosan-coated silicon dioxide (CS/S)	765:802	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	magnetite					825:833	chitosan-coated magnetite	809:833	chitosan-coated magnetite (CS/M)	809:840	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	CS/M/S					756:761	CS/M/S	756:761	CS/M/S	756:761	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	CS/M					836:839	CS/M	836:839	CS/M	836:839	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	CS/S					798:801	CS/S	798:801	CS/S	798:801	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	oxide					690:694	graphene oxide	681:694	graphene oxide	681:694	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	magnetite					725:733	chitosan-coated magnetite	709:733	chitosan-coated magnetite	709:733	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	5	dep	[chitosan-coated	632:647	arg1	magnetite					649:657	magnetite	649:657	magnetite	649:657	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	5	6	theme	prepared	862:869	arg1	nanocomposites					871:884	The prepared nanocomposites	858:884	The prepared nanocomposites	858:884	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	9	7	theme	While	1482:1486	arg1	potentiality					1502:1513	the releasing potentiality	1488:1513	While the releasing potentiality for CS/M composite	1482:1532	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	9	7	theme	While	1482:1486	arg1	highest					1542:1548	highest	1542:1548	highest	1542:1548	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	6	8	theme	selected	1169:1176	arg1	nanocomposite					1202:1214	a selected chitosan-functionalized nanocomposite	1167:1214	a selected chitosan-functionalized nanocomposite	1167:1214	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	3	9	from	potentiality	482:493	arg1	CS					508:509	CS	508:509	CS	508:509	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	3	9	from	potentiality	482:493	arg1	chitosan					498:505	chitosan	498:505	chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles	498:604	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	4	10	theme	[chitosan-coated	632:647	arg1	nanocomposites					617:630	Different nanocomposites	607:630	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)]	607:841	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	11	theme	silicon	660:666	arg1	dioxide					668:674	silicon dioxide	660:674	silicon dioxide	660:674	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	5	12	theme	transmission	999:1010	arg1	TEM					1033:1035	TEM	1033:1035	TEM	1033:1035	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	5	12	theme	transmission	999:1010	arg1	microscopy					1021:1030	transmission electron microscopy	999:1030	transmission electron microscopy (TEM)	999:1036	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	4	13	theme	chitosan-coated	765:779	arg1	CS/S					798:801	CS/S	798:801	CS/S	798:801	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	13	theme	chitosan-coated	765:779	arg1	dioxide					789:795	chitosan-coated silicon dioxide	765:795	chitosan-coated silicon dioxide (CS/S)	765:802	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	7	14	theme	prepared	1326:1333	arg1	nanocomposites					1335:1348	the prepared nanocomposites	1322:1348	the prepared nanocomposites	1322:1348	The UV-Vis spectroscopy was used to study cisP loading and release from the prepared nanocomposites.					
32179117	7	15	theme	UV-Vis	1254:1259	arg1	spectroscopy					1261:1272	The UV-Vis spectroscopy	1250:1272	The UV-Vis spectroscopy	1250:1272	The UV-Vis spectroscopy was used to study cisP loading and release from the prepared nanocomposites.					
32179117	5	16	dep	Fourier	933:939	arg1	transform					941:949	transform	941:949	transform infrared	941:958	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	5	17	theme	electron	1012:1019	arg1	TEM					1033:1035	TEM	1033:1035	TEM	1033:1035	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	5	17	theme	electron	1012:1019	arg1	microscopy					1021:1030	transmission electron microscopy	999:1030	transmission electron microscopy (TEM)	999:1036	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	9	18	theme	CS/M	1519:1522	arg1	composite					1524:1532	CS/M composite	1519:1532	CS/M composite	1519:1532	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	0	19	theme	Chitosan	0:7	arg1	nanocomposites					57:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites	0:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.	0:161	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	19	theme	Chitosan	0:7	arg1	efficiency					102:111	efficiency	102:111	efficiency as cisplatin drug delivery	102:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	19	theme	Chitosan	0:7	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	19	theme	Chitosan	0:7	arg1	Synthesis					73:81	Synthesis	73:81	Synthesis	73:81	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	5	20	dep	transform	941:949	arg1	infrared					951:958	infrared	951:958	transform infrared	941:958	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	6	21	theme	cisP	1157:1160	arg1	mechanism					1144:1152	the interaction mechanism	1128:1152	the interaction mechanism of cisP	1128:1160	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	8	22	theme	CS/M/S/GO	1429:1437	arg1	nanocomposites					1439:1452	CS/M/S/GO nanocomposites	1429:1452	CS/M/S/GO nanocomposites (87% and 84% respectively)	1429:1479	The results showed that the highest loading efficacy was achieved by CS/M and CS/M/S/GO nanocomposites (87% and 84% respectively).					
32179117	1	23	theme	Drug	163:166	arg1	systems					177:183	Drug delivery systems	163:183	Drug delivery systems with controlled release	163:207	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
32179117	1	24	with	systems	177:183	arg1	release					201:207	controlled release	190:207	controlled release	190:207	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
32179117	4	25	theme	silicon	781:787	arg1	CS/S					798:801	CS/S	798:801	CS/S	798:801	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	25	theme	silicon	781:787	arg1	dioxide					789:795	chitosan-coated silicon dioxide	765:795	chitosan-coated silicon dioxide (CS/S)	765:802	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	0	26	dep	nanocomposites	57:70	arg1	nanocomposites					57:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites	0:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.	0:161	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	26	dep	nanocomposites	57:70	arg1	efficiency					102:111	efficiency	102:111	efficiency as cisplatin drug delivery	102:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	26	dep	nanocomposites	57:70	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	26	dep	nanocomposites	57:70	arg1	calculations					149:160	DFT calculations	145:160	DFT calculations	145:160	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	26	dep	nanocomposites	57:70	arg1	Synthesis					73:81	Synthesis	73:81	Synthesis	73:81	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	1	27	theme	delivery	168:175	arg1	systems					177:183	Drug delivery systems	163:183	Drug delivery systems with controlled release	163:207	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
32179117	1	28	theme	various	267:273	arg1	diseases					275:282	various diseases	267:282	various diseases	267:282	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
32179117	3	29	from	efficacy	461:468	arg1	CS					508:509	CS	508:509	CS	508:509	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	3	29	from	efficacy	461:468	arg1	chitosan					498:505	chitosan	498:505	chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles	498:604	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	3	30	theme	cisplatin	436:444	arg1	efficacy					461:468	cisplatin (cisP) loading efficacy	436:468	cisplatin (cisP) loading efficacy	436:468	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	0	31	theme	magnetite	10:18	arg1	nanocomposites					57:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites	0:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.	0:161	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	31	theme	magnetite	10:18	arg1	efficiency					102:111	efficiency	102:111	efficiency as cisplatin drug delivery	102:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	31	theme	magnetite	10:18	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	31	theme	magnetite	10:18	arg1	Synthesis					73:81	Synthesis	73:81	Synthesis	73:81	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	1	32	theme	diseases	275:282	arg1	treatment					254:262	the treatment	250:262	the treatment of various diseases	250:282	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
32179117	3	33	theme	graphene	572:579	arg1	oxide					581:585	graphene oxide	572:585	graphene oxide (GO)	572:590	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	4	34	theme	graphene	681:688	arg1	oxide					690:694	graphene oxide	681:694	graphene oxide	681:694	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	2	35	theme	specific	388:395	arg1	delivery					402:409	specific site delivery	388:409	specific site delivery	388:409	The efficacy of the drug can be enhanced by increasing its solubility, stability, bioavailability, and specific site delivery.					
32179117	0	36	theme	dioxide	29:35	arg1	nanocomposites					57:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites	0:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.	0:161	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	36	theme	dioxide	29:35	arg1	efficiency					102:111	efficiency	102:111	efficiency as cisplatin drug delivery	102:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	36	theme	dioxide	29:35	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	36	theme	dioxide	29:35	arg1	Synthesis					73:81	Synthesis	73:81	Synthesis	73:81	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	37	theme	drug	126:129	arg1	delivery					131:138	cisplatin drug delivery	116:138	cisplatin drug delivery	116:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	5	38	theme	scanning	969:976	arg1	microscopy					987:996	scanning electron microscopy	969:996	scanning electron microscopy	969:996	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	7	39	theme	cisP	1292:1295	arg1	loading					1297:1303	cisP loading and release	1292:1315	loading	1297:1303	The UV-Vis spectroscopy was used to study cisP loading and release from the prepared nanocomposites.					
32179117	1	40	theme	controlled	190:199	arg1	release					201:207	controlled release	190:207	controlled release	190:207	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
32179117	0	41	theme	silicon	21:27	arg1	dioxide					29:35	silicon dioxide	21:35	silicon dioxide	21:35	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	5	42	theme	energy-dispersive	1042:1058	arg1	spectroscopy					1066:1077	energy-dispersive X-ray spectroscopy	1042:1077	energy-dispersive X-ray spectroscopy (EDS)	1042:1083	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	5	42	theme	energy-dispersive	1042:1058	arg1	EDS					1080:1082	EDS	1080:1082	EDS	1080:1082	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	0	43	theme	cisplatin	116:124	arg1	delivery					131:138	cisplatin drug delivery	116:138	cisplatin drug delivery	116:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	5	44	theme	electron	978:985	arg1	microscopy					987:996	scanning electron microscopy	969:996	scanning electron microscopy	969:996	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	3	45	theme	silicon	547:553	arg1	S					564:564	S	564:564	S	564:564	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	3	45	theme	silicon	547:553	arg1	dioxide					555:561	silicon dioxide	547:561	silicon dioxide (S)	547:565	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	5	46	theme	X-ray	1060:1064	arg1	spectroscopy					1066:1077	energy-dispersive X-ray spectroscopy	1042:1077	energy-dispersive X-ray spectroscopy (EDS)	1042:1083	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	5	46	theme	X-ray	1060:1064	arg1	EDS					1080:1082	EDS	1080:1082	EDS	1080:1082	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	4	47	theme	chitosan-coated	809:823	arg1	magnetite					825:833	chitosan-coated magnetite	809:833	chitosan-coated magnetite (CS/M)	809:840	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	47	theme	chitosan-coated	809:823	arg1	CS/M					836:839	CS/M	836:839	CS/M	836:839	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	6	48	theme	gas	1223:1225	arg1	phase					1227:1231	the gas phase	1219:1231	the gas phase	1219:1231	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	3	49	theme	loading	453:459	arg1	efficacy					461:468	cisplatin (cisP) loading efficacy	436:468	cisplatin (cisP) loading efficacy	436:468	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	6	50	theme	water	1237:1241	arg1	media					1243:1247	water media	1237:1247	water media	1237:1247	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	8	51	theme	loading	1387:1393	arg1	efficacy					1395:1402	the highest loading efficacy	1375:1402	the highest loading efficacy	1375:1402	The results showed that the highest loading efficacy was achieved by CS/M and CS/M/S/GO nanocomposites (87% and 84% respectively).					
32179117	7	52	used	used	1278:1281	arg2	spectroscopy					1261:1272	The UV-Vis spectroscopy	1250:1272	The UV-Vis spectroscopy	1250:1272	The UV-Vis spectroscopy was used to study cisP loading and release from the prepared nanocomposites.					
32179117	0	53	theme	DFT	145:147	arg1	calculations					149:160	DFT calculations	145:160	DFT calculations	145:160	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	8	54	theme	highest	1379:1385	arg1	efficacy					1395:1402	the highest loading efficacy	1375:1402	the highest loading efficacy	1375:1402	The results showed that the highest loading efficacy was achieved by CS/M and CS/M/S/GO nanocomposites (87% and 84% respectively).					
32179117	5	55	theme	X-ray	908:912	arg1	XRD					927:929	XRD	927:929	XRD	927:929	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	5	55	theme	X-ray	908:912	arg1	diffraction					914:924	X-ray diffraction	908:924	X-ray diffraction (XRD)	908:930	The prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).					
32179117	6	56	theme	interaction	1132:1142	arg1	mechanism					1144:1152	the interaction mechanism	1128:1152	the interaction mechanism of cisP	1128:1160	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	3	57	theme	cisP	447:450	arg1	efficacy					461:468	cisplatin (cisP) loading efficacy	436:468	cisplatin (cisP) loading efficacy	436:468	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	4	58	theme	chitosan-coated	709:723	arg1	CS/M/S					756:761	CS/M/S	756:761	CS/M/S	756:761	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	4	58	theme	chitosan-coated	709:723	arg1	magnetite					725:733	chitosan-coated magnetite	709:733	chitosan-coated magnetite	709:733	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	2	59	theme	drug	305:308	arg1	efficacy					289:296	The efficacy	285:296	The efficacy of the drug	285:308	The efficacy of the drug can be enhanced by increasing its solubility, stability, bioavailability, and specific site delivery.					
32179117	0	60	theme	oxide	51:55	arg1	nanocomposites					57:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites	0:70	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.	0:161	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	60	theme	oxide	51:55	arg1	efficiency					102:111	efficiency	102:111	efficiency as cisplatin drug delivery	102:138	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	60	theme	oxide	51:55	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	0	60	theme	oxide	51:55	arg1	Synthesis					73:81	Synthesis	73:81	Synthesis	73:81	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	4	61	theme	silicon	739:745	arg1	dioxide					747:753	silicon dioxide	739:753	silicon dioxide	739:753	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	2	62	theme	site	397:400	arg1	delivery					402:409	specific site delivery	388:409	specific site delivery	388:409	The efficacy of the drug can be enhanced by increasing its solubility, stability, bioavailability, and specific site delivery.					
32179117	4	63	theme	Different	607:615	arg1	nanocomposites					617:630	Different nanocomposites	607:630	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)]	607:841	Different nanocomposites [chitosan-coated magnetite, silicon dioxide, and graphene oxide (CS/M/S/GO); chitosan-coated magnetite and silicon dioxide (CS/M/S); chitosan-coated silicon dioxide (CS/S); and chitosan-coated magnetite (CS/M)] were prepared.					
32179117	8	64	dep	nanocomposites	1439:1452	arg1	%					1457:1457	87%	1455:1457	87%	1455:1457	The results showed that the highest loading efficacy was achieved by CS/M and CS/M/S/GO nanocomposites (87% and 84% respectively).					
32179117	8	64	dep	nanocomposites	1439:1452	arg1	%					1465:1465	84%	1463:1465	84%	1463:1465	The results showed that the highest loading efficacy was achieved by CS/M and CS/M/S/GO nanocomposites (87% and 84% respectively).					
32179117	0	65	theme	graphene	42:49	arg1	oxide					51:55	graphene oxide	42:55	graphene oxide	42:55	Chitosan, magnetite, silicon dioxide, and graphene oxide nanocomposites: Synthesis, characterization, efficiency as cisplatin drug delivery, and DFT calculations.					
32179117	9	66	theme	other	1568:1572	arg1	ones					1574:1577	the other ones	1564:1577	the other ones (91%)	1564:1583	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	9	66	theme	other	1568:1572	arg1	%					1582:1582	91%	1580:1582	91%	1580:1582	While the releasing potentiality for CS/M composite was the highest compared with the other ones (91%).					
32179117	3	67	dep	oxide	581:585	arg1	GO					588:589	GO	588:589	GO	588:589	Herein, we investigated cisplatin (cisP) loading efficacy and release potentiality on chitosan (CS) functionalized with magnetite (M), silicon dioxide (S), and graphene oxide (GO) nanoparticles.					
32179117	6	68	theme	DFT	1086:1088	arg1	calculations					1090:1101	DFT calculations	1086:1101	DFT calculations	1086:1101	DFT calculations were employed to explore the interaction mechanism of cisP with a selected chitosan-functionalized nanocomposite in the gas phase and water media.					
32179117	1	69	theme	important	230:238	arg1	tools					240:244	important tools	230:244	important tools for the treatment of various diseases	230:282	Drug delivery systems with controlled release have been considered important tools for the treatment of various diseases.					
34152738	7	0	theme	micro-CT	1677:1684	arg1	analyses					1686:1693	micro-CT analyses	1677:1693	micro-CT analyses	1677:1693	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	6	1	theme	mechanical	1453:1462	arg1	stress					1464:1469	mechanical stress	1453:1469	mechanical stress	1453:1469	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	6	2	theme	extracellular	1348:1360	arg1	ECM					1370:1372	ECM	1370:1372	ECM	1370:1372	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	6	2	theme	extracellular	1348:1360	arg1	matrix					1362:1367	extracellular matrix	1348:1367	extracellular matrix (ECM) properties	1348:1384	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	8	3	theme	optimized	1926:1934	arg1	scaffold					1936:1943	the optimized scaffold	1922:1943	the optimized scaffold	1922:1943	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	8	3	theme	optimized	1926:1934	arg1	adjuvant					1958:1965	adjuvant	1958:1965	adjuvant	1958:1965	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	3	4	theme	cells	574:578	arg1	priming					539:545	the priming	535:545	the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration	535:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	7	5	theme	in	1656:1657	arg1	experiments					1664:1674	in vivo experiments	1656:1674	in vivo experiments	1656:1674	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	5	6	with	MSCs	1151:1154	arg1	expression					1219:1228	significantly increased expression	1195:1228	significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression	1195:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	3	7	theme	lineage-specific	594:609	arg1	differentiation					611:625	lineage-specific differentiation	594:625	lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration	594:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	4	8	theme	swift	980:984	arg1	regeneration					991:1002	swift bone regeneration	980:1002	swift bone regeneration	980:1002	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	6	9	theme	favorable	1398:1406	arg1	microenvironment					1408:1423	a favorable microenvironment	1396:1423	a favorable microenvironment	1396:1423	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	6	9	theme	favorable	1398:1406	arg1	support					1437:1443	structural support	1426:1443	structural support against mechanical stress	1426:1469	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	1	10	theme	critical-sized	223:236	arg1	defects					243:249	critical-sized bone defects	223:249	critical-sized bone defects	223:249	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	7	11	theme	ossified	1858:1865	arg1	tissue					1867:1872	new ossified tissue	1854:1872	new ossified tissue	1854:1872	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	0	12	theme	Bone	147:150	arg1	Regeneration					152:163	Guided Bone Regeneration	140:163	Guided Bone Regeneration in a Critical-Sized Calvarial Defect	140:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	1	13	theme	defects	243:249	arg1	regeneration					207:218	The regeneration	203:218	The regeneration of critical-sized bone defects with biomimetic scaffolds	203:275	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	7	14	from	tissue	1867:1872	arg1	defects					1888:1894	4 mm sized defects	1877:1894	4 mm sized defects	1877:1894	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	5	15	theme	alkaline	1233:1240	arg1	phosphatase					1242:1252	alkaline phosphatase	1233:1252	alkaline phosphatase	1233:1252	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	3	16	theme	varied	687:692	arg1	cues					725:728	varied mechanochemical and biological cues	687:728	varied mechanochemical and biological cues during bone tissue regeneration	687:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	1	17	theme	biomimetic	256:265	arg1	scaffolds					267:275	biomimetic scaffolds	256:275	biomimetic scaffolds	256:275	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	7	18	theme	bone	1835:1838	arg1	formation					1840:1848	enhanced bone formation	1826:1848	enhanced bone formation	1826:1848	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	4	19	theme	defect	1019:1024	arg1	model					1038:1042	a calvarial defect BALB/c mice model	1007:1042	a calvarial defect BALB/c mice model	1007:1042	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	20	theme	optimized	806:814	arg1	gallate-N					864:872	adenosine/epigallocatechin gallate-N	837:872	adenosine/epigallocatechin gallate-N	837:872	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	20	theme	optimized	806:814	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	20	theme	optimized	806:814	arg1	scaffold					827:834	an optimized osteogenic scaffold	803:834	an optimized osteogenic scaffold	803:834	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	0	21	theme	Critical-Sized	170:183	arg1	Defect					195:200	a Critical-Sized Calvarial Defect	168:200	a Critical-Sized Calvarial Defect	168:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	4	22	theme	mice	1033:1036	arg1	model					1038:1042	a calvarial defect BALB/c mice model	1007:1042	a calvarial defect BALB/c mice model	1007:1042	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	8	23	theme	tissue	1981:1986	arg1	regeneration					1988:1999	bone tissue regeneration	1976:1999	bone tissue regeneration	1976:1999	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	5	24	theme	ex	1049:1050	arg1	findings					1057:1064	The ex vivo findings	1045:1064	The ex vivo findings	1045:1064	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	7	25	theme	bone	1810:1813	arg1	union					1815:1819	effectual bone union	1800:1819	effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects	1800:1894	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	0	26	theme	Integrative	81:91	arg1	Scaffold					124:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold	74:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect	74:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	5	27	theme	osteocalcin	1286:1296	arg1	expression					1298:1307	enhanced osteocalcin expression	1277:1307	enhanced osteocalcin expression	1277:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	7	28	with	union	1815:1819	arg1	tissue					1867:1872	new ossified tissue	1854:1872	new ossified tissue	1854:1872	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	7	28	with	union	1815:1819	arg1	formation					1840:1848	enhanced bone formation	1826:1848	enhanced bone formation	1826:1848	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	4	29	theme	chitosan/collagen	890:906	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	29	theme	chitosan/collagen	890:906	arg1	I					936:936	AD/EGCG-g-NOCC@clgn I	916:936	AD/EGCG-g-NOCC@clgn I	916:936	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	29	theme	chitosan/collagen	890:906	arg1	scaffold					827:834	an optimized osteogenic scaffold	803:834	an optimized osteogenic scaffold	803:834	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	0	30	theme	Composite	114:122	arg1	Scaffold					124:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold	74:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect	74:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	5	31	theme	pro-osteogenic	1091:1104	arg1	potential					1106:1114	the pro-osteogenic potential	1087:1114	the pro-osteogenic potential of adenosine and EGCG	1087:1136	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	5	32	theme	deposits	1263:1270	arg1	expression					1219:1228	significantly increased expression	1195:1228	significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression	1195:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	8	33	theme	therapeutic	2052:2062	arg1	efficacy					2064:2071	promising therapeutic efficacy	2042:2071	promising therapeutic efficacy	2042:2071	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	7	34	theme	sized	1882:1886	arg1	defects					1888:1894	4 mm sized defects	1877:1894	4 mm sized defects	1877:1894	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	4	35	theme	adenosine/epigallocatechin	837:862	arg1	gallate-N					864:872	adenosine/epigallocatechin gallate-N	837:872	adenosine/epigallocatechin gallate-N	837:872	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	35	theme	adenosine/epigallocatechin	837:862	arg1	scaffold					827:834	an optimized osteogenic scaffold	803:834	an optimized osteogenic scaffold	803:834	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	2	36	theme	temporal	445:452	arg1	regulation					454:463	temporal regulation	445:463	temporal regulation	445:463	Two confounding mechanisms, efficacy manipulation, and temporal regulation dictate the scaffold's bone regenerative ability.					
34152738	0	37	theme	Control	0:6	arg1	Release					8:14	Control Release	0:14	Control Release of Adenosine	0:27	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	7	38	theme	4	1877:1877	arg1	mm					1879:1880	mm	1879:1880	mm	1879:1880	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	2	39	theme	bone	488:491	arg1	ability					506:512	the scaffold's bone regenerative ability	473:512	the scaffold's bone regenerative ability	473:512	Two confounding mechanisms, efficacy manipulation, and temporal regulation dictate the scaffold's bone regenerative ability.					
34152738	3	40	theme	bone	737:740	arg1	regeneration					749:760	bone tissue regeneration	737:760	bone tissue regeneration	737:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	3	41	theme	mesenchymal	554:564	arg1	MSCs					581:584	MSCs	581:584	MSCs	581:584	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	3	41	theme	mesenchymal	554:564	arg1	cells					574:578	the mesenchymal stromal cells	550:578	the mesenchymal stromal cells (MSCs)	550:585	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	1	42	theme	osteogenic	369:378	arg1	activity					380:387	altered osteogenic activity	361:387	altered osteogenic activity	361:387	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	5	43	theme	osteoblast	1163:1172	arg1	differentiation					1174:1188	osteoblast differentiation	1163:1188	osteoblast differentiation	1163:1188	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	4	44	theme	AD/EGCG-g-NOCC	916:929	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	44	theme	AD/EGCG-g-NOCC	916:929	arg1	I					936:936	AD/EGCG-g-NOCC@clgn I	916:936	AD/EGCG-g-NOCC@clgn I	916:936	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	1	45	with	regeneration	207:218	arg1	scaffolds					267:275	biomimetic scaffolds	256:275	biomimetic scaffolds	256:275	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	6	46	theme	matrix	1608:1613	arg1	osteointegration					1615:1630	matrix osteointegration	1608:1630	matrix osteointegration observed	1608:1639	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	4	47	theme	clgn	931:934	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	47	theme	clgn	931:934	arg1	I					936:936	AD/EGCG-g-NOCC@clgn I	916:936	AD/EGCG-g-NOCC@clgn I	916:936	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	3	48	dep	priming	539:545	arg1	critical					523:530	critical	523:530	critical	523:530	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	6	49	theme	cell	1550:1553	arg1	differentiation					1555:1569	cell differentiation	1550:1569	cell differentiation	1550:1569	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	2	50	theme	efficacy	418:425	arg1	manipulation					427:438	efficacy manipulation	418:438	efficacy manipulation	418:438	Two confounding mechanisms, efficacy manipulation, and temporal regulation dictate the scaffold's bone regenerative ability.					
34152738	4	51	theme	bone	986:989	arg1	regeneration					991:1002	swift bone regeneration	980:1002	swift bone regeneration	980:1002	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	1	52	theme	avascular	315:323	arg1	necrosis					325:332	avascular necrosis	315:332	avascular necrosis	315:332	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	6	53	theme	structural	1426:1435	arg1	microenvironment					1408:1423	a favorable microenvironment	1396:1423	a favorable microenvironment	1396:1423	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	6	53	theme	structural	1426:1435	arg1	support					1437:1443	structural support	1426:1443	structural support against mechanical stress	1426:1469	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	4	54	theme	osteoinductive	947:960	arg1	components					962:971	osteoinductive components	947:971	osteoinductive components toward swift bone regeneration	947:1002	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	6	55	theme	3D	1324:1325	arg1	matrix					1327:1332	the 3D matrix	1320:1332	the 3D matrix	1320:1332	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	6	55	theme	3D	1324:1325	arg1	reservoir					1486:1494	a reservoir	1484:1494	a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed	1484:1639	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	7	56	theme	new	1854:1856	arg1	tissue					1867:1872	new ossified tissue	1854:1872	new ossified tissue	1854:1872	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	6	57	theme	sustained	1500:1508	arg1	release					1510:1516	sustained release	1500:1516	sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed	1500:1639	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	3	58	theme	bone-forming	632:643	arg1	osteoblast					645:654	bone-forming osteoblast	632:654	bone-forming osteoblast	632:654	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	8	59	theme	calvarial	2019:2027	arg1	defects					2029:2035	critical-sized calvarial defects	2004:2035	critical-sized calvarial defects with promising therapeutic efficacy	2004:2071	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	1	60	theme	bone	238:241	arg1	defects					243:249	critical-sized bone defects	223:249	critical-sized bone defects	223:249	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	2	61	theme	confounding	394:404	arg1	mechanisms					406:415	Two confounding mechanisms	390:415	Two confounding mechanisms	390:415	Two confounding mechanisms, efficacy manipulation, and temporal regulation dictate the scaffold's bone regenerative ability.					
34152738	7	62	from	experiments	1664:1674	arg1	histology					1696:1704	histology	1696:1704	histology	1696:1704	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	7	62	from	experiments	1664:1674	arg1	histomorphometry					1711:1726	histomorphometry	1711:1726	histomorphometry	1711:1726	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	7	62	from	experiments	1664:1674	arg1	analyses					1686:1693	micro-CT analyses	1677:1693	micro-CT analyses	1677:1693	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	7	62	from	experiments	1664:1674	arg1	Evidence					1642:1649	Evidence	1642:1649	Evidence from in vivo experiments	1642:1674	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	7	63	theme	enhanced	1826:1833	arg1	formation					1840:1848	enhanced bone formation	1826:1848	enhanced bone formation	1826:1848	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	0	64	theme	Calvarial	185:193	arg1	Defect					195:200	a Critical-Sized Calvarial Defect	168:200	a Critical-Sized Calvarial Defect	168:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	5	65	theme	phosphatase	1242:1252	arg1	expression					1219:1228	significantly increased expression	1195:1228	significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression	1195:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	4	66	theme	calvarial	1009:1017	arg1	model					1038:1042	a calvarial defect BALB/c mice model	1007:1042	a calvarial defect BALB/c mice model	1007:1042	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	3	67	theme	biological	714:723	arg1	cues					725:728	varied mechanochemical and biological cues	687:728	varied mechanochemical and biological cues during bone tissue regeneration	687:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	7	68	theme	effectual	1800:1808	arg1	union					1815:1819	effectual bone union	1800:1819	effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects	1800:1894	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	4	69	theme	BALB/c	1026:1031	arg1	model					1038:1042	a calvarial defect BALB/c mice model	1007:1042	a calvarial defect BALB/c mice model	1007:1042	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	70	theme	osteogenic	816:825	arg1	gallate-N					864:872	adenosine/epigallocatechin gallate-N	837:872	adenosine/epigallocatechin gallate-N	837:872	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	70	theme	osteogenic	816:825	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	70	theme	osteogenic	816:825	arg1	scaffold					827:834	an optimized osteogenic scaffold	803:834	an optimized osteogenic scaffold	803:834	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	0	71	theme	Bone	76:79	arg1	Scaffold					124:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold	74:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect	74:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	5	72	theme	increased	1209:1217	arg1	expression					1219:1228	significantly increased expression	1195:1228	significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression	1195:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	3	73	theme	mechanochemical	694:708	arg1	cues					725:728	varied mechanochemical and biological cues	687:728	varied mechanochemical and biological cues during bone tissue regeneration	687:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	8	74	theme	bone	1976:1979	arg1	regeneration					1988:1999	bone tissue regeneration	1976:1999	bone tissue regeneration	1976:1999	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	6	75	theme	matrix	1362:1367	arg1	properties					1375:1384	extracellular matrix (ECM) properties	1348:1384	extracellular matrix (ECM) properties	1348:1384	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	6	76	theme	osteoinductive	1521:1534	arg1	molecules					1536:1544	osteoinductive molecules	1521:1544	osteoinductive molecules for cell differentiation, proliferation, and migration	1521:1599	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	0	77	theme	EGCG-g-NOCC/Collagen	93:112	arg1	Scaffold					124:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold	74:131	a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect	74:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	5	78	theme	enhanced	1277:1284	arg1	expression					1298:1307	enhanced osteocalcin expression	1277:1307	enhanced osteocalcin expression	1277:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	6	79	theme	molecules	1536:1544	arg1	release					1510:1516	sustained release	1500:1516	sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed	1500:1639	Moreover, the 3D matrix recapitulates extracellular matrix (ECM) properties, provides a favorable microenvironment, structural support against mechanical stress, and acts as a reservoir for sustained release of osteoinductive molecules for cell differentiation, proliferation, and migration during matrix osteointegration observed.					
34152738	5	80	theme	expression	1298:1307	arg1	expression					1219:1228	significantly increased expression	1195:1228	significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression	1195:1307	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	8	81	theme	critical-sized	2004:2017	arg1	defects					2029:2035	critical-sized calvarial defects	2004:2035	critical-sized calvarial defects with promising therapeutic efficacy	2004:2071	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	5	82	dep	ex	1049:1050	arg1	vivo					1052:1055	vivo	1052:1055	vivo	1052:1055	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	4	83	theme	O-carboxymethyl	874:888	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	83	theme	O-carboxymethyl	874:888	arg1	I					936:936	AD/EGCG-g-NOCC@clgn I	916:936	AD/EGCG-g-NOCC@clgn I	916:936	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	83	theme	O-carboxymethyl	874:888	arg1	scaffold					827:834	an optimized osteogenic scaffold	803:834	an optimized osteogenic scaffold	803:834	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	0	84	theme	Guided	140:145	arg1	Regeneration					152:163	Guided Bone Regeneration	140:163	Guided Bone Regeneration in a Critical-Sized Calvarial Defect	140:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	5	85	theme	calcium	1255:1261	arg1	deposits					1263:1270	calcium deposits	1255:1270	calcium deposits	1255:1270	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	7	86	from	formation	1840:1848	arg1	defects					1888:1894	4 mm sized defects	1877:1894	4 mm sized defects	1877:1894	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	8	87	theme	promising	2042:2050	arg1	efficacy					2064:2071	promising therapeutic efficacy	2042:2071	promising therapeutic efficacy	2042:2071	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	0	88	theme	Adenosine	19:27	arg1	Release					8:14	Control Release	0:14	Control Release of Adenosine	0:27	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	1	89	theme	chronic	335:341	arg1	inflammation					343:354	chronic inflammation	335:354	chronic inflammation	335:354	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	7	90	theme	mm	1879:1880	arg1	defects					1888:1894	4 mm sized defects	1877:1894	4 mm sized defects	1877:1894	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	2	91	theme	regenerative	493:504	arg1	ability					506:512	the scaffold's bone regenerative ability	473:512	the scaffold's bone regenerative ability	473:512	Two confounding mechanisms, efficacy manipulation, and temporal regulation dictate the scaffold's bone regenerative ability.					
34152738	3	92	theme	tissue	742:747	arg1	regeneration					749:760	bone tissue regeneration	737:760	bone tissue regeneration	737:760	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	0	93	theme	Osteogenic	40:49	arg1	Differentiation					51:65	Osteogenic Differentiation	40:65	Osteogenic Differentiation	40:65	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	7	94	theme	accelerated	1736:1746	arg1	osteogenesis					1748:1759	accelerated osteogenesis	1736:1759	accelerated osteogenesis	1736:1759	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	1	95	theme	altered	361:367	arg1	activity					380:387	altered osteogenic activity	361:387	altered osteogenic activity	361:387	The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity.					
34152738	3	96	theme	stromal	566:572	arg1	MSCs					581:584	MSCs	581:584	MSCs	581:584	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	3	96	theme	stromal	566:572	arg1	cells					574:578	the mesenchymal stromal cells	550:578	the mesenchymal stromal cells (MSCs)	550:585	Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration.					
34152738	7	97	dep	in	1656:1657	arg1	vivo					1659:1662	vivo	1659:1662	vivo	1659:1662	Evidence from in vivo experiments, micro-CT analyses, histology, and histomorphometry signify accelerated osteogenesis both qualitatively and quantitatively: effectual bone union with enhanced bone formation and new ossified tissue in 4 mm sized defects.					
34152738	5	98	theme	adenosine	1119:1127	arg1	potential					1106:1114	the pro-osteogenic potential	1087:1114	the pro-osteogenic potential of adenosine and EGCG	1087:1136	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	5	99	theme	EGCG	1133:1136	arg1	potential					1106:1114	the pro-osteogenic potential	1087:1114	the pro-osteogenic potential of adenosine and EGCG	1087:1136	The ex vivo findings distinctly establish the pro-osteogenic potential of adenosine and EGCG, stimulating MSCs toward osteoblast differentiation with significantly increased expression of alkaline phosphatase, calcium deposits, and enhanced osteocalcin expression.					
34152738	0	100	from	Regeneration	152:163	arg1	Defect					195:200	a Critical-Sized Calvarial Defect	168:200	a Critical-Sized Calvarial Defect	168:200	Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG-g-NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.					
34152738	8	101	with	defects	2029:2035	arg1	efficacy					2064:2071	promising therapeutic efficacy	2042:2071	promising therapeutic efficacy	2042:2071	Our results suggest that the optimized scaffold serves as an adjuvant to guide bone tissue regeneration in critical-sized calvarial defects with promising therapeutic efficacy.					
34152738	4	102	theme	@	930:930	arg1	type					908:911	O-carboxymethyl chitosan/collagen type I	874:913	O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I)	874:937	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
34152738	4	102	theme	@	930:930	arg1	I					936:936	AD/EGCG-g-NOCC@clgn I	916:936	AD/EGCG-g-NOCC@clgn I	916:936	This study sought to design and develop an optimized osteogenic scaffold, adenosine/epigallocatechin gallate-N,O-carboxymethyl chitosan/collagen type I (AD/EGCG-g-NOCC@clgn I), having osteoinductive components toward swift bone regeneration in a calvarial defect BALB/c mice model.					
32255604	4	0	theme	tumor	757:761	arg1	spheroids					763:771	reproducible, uniform tumor spheroids	735:771	reproducible, uniform tumor spheroids	735:771	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	9	1	theme	precision	1557:1565	arg1	oncology					1567:1574	precision oncology	1557:1574	precision oncology	1557:1574	Since this 3D microarchitecture typifies ECM bioautomaton, this matrix can also be wielded for precision oncology.					
32255604	4	2	theme	human	813:817	arg1	DLD-1					835:839	DLD-1	835:839	DLD-1	835:839	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	4	2	theme	human	813:817	arg1	adenocarcinoma					819:832	human adenocarcinoma	813:832	human adenocarcinoma (DLD-1)	813:840	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	6	3	theme	reduced	1094:1100	arg1	efficacy					1102:1109	Etoposide's reduced efficacy	1082:1109	Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix	1082:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	5	4	theme	drug	983:986	arg1	screening					988:996	anticancer drug screening	972:996	anticancer drug screening	972:996	Thereafter, their capacity for anticancer drug screening was examined using limited cancer drugs.					
32255604	3	5	dep	heterogeneous	492:504	arg1	fibroporous					507:517	fibroporous	507:517	fibroporous	507:517	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	8	6	theme	composite	1451:1459	arg1	transparency					1419:1430	the exceptional transparency	1403:1430	the exceptional transparency of the synthesized composite	1403:1459	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	2	7	theme	quasi-spherical	286:300	arg1	porogen					302:308	a quasi-spherical porogen	284:308	a quasi-spherical porogen	284:308	Herein, water behaved as a quasi-spherical porogen, for engineering polysaccharide-rich and chemically defined 3D-microarchitecture, with semi-interpenetrating networks (S-IPNs).					
32255604	6	8	theme	conflicting	1043:1053	arg1	results					1070:1076	The conflicting drug screening results	1039:1076	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix	1039:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	6	9	theme	decreased	1180:1188	arg1	access					1195:1200	decreased drug access	1180:1200	decreased drug access	1180:1200	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	4	10	theme	human	861:865	arg1	cells					880:884	human glioblastoma cells	861:884	human glioblastoma cells	861:884	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	6	11	theme	drug	1055:1058	arg1	results					1070:1076	The conflicting drug screening results	1039:1076	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix	1039:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	3	12	theme	bioartificial	579:591	arg1	scaffolds					593:601	the bioartificial scaffolds	575:601	the bioartificial scaffolds	575:601	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	1	13	theme	water	183:187	arg1	molecules					189:197	water molecules	183:197	water molecules	183:197	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	9	14	theme	ECM	1503:1505	arg1	bioautomaton					1507:1518	ECM bioautomaton	1503:1518	ECM bioautomaton	1503:1518	Since this 3D microarchitecture typifies ECM bioautomaton, this matrix can also be wielded for precision oncology.					
32255604	8	15	theme	real-time	1332:1340	arg1	oncology					1378:1385	real-time, high-content, phenotypic precision oncology	1332:1385	real-time, high-content, phenotypic precision oncology	1332:1385	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	8	16	theme	precision	1368:1376	arg1	oncology					1378:1385	real-time, high-content, phenotypic precision oncology	1332:1385	real-time, high-content, phenotypic precision oncology	1332:1385	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	3	17	theme	scaffolds	593:601	arg1	self-remodeling					556:570	self-remodeling	556:570	self-remodeling	556:570	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	1	18	theme	molecules	189:197	arg1	shape					174:178	the "quasi-spherical" shape	152:178	the "quasi-spherical" shape of water molecules	152:197	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	8	19	theme	synthesized	1439:1449	arg1	composite					1451:1459	the synthesized composite	1435:1459	the synthesized composite	1435:1459	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	8	20	theme	exceptional	1407:1417	arg1	transparency					1419:1430	the exceptional transparency	1403:1430	the exceptional transparency of the synthesized composite	1403:1459	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	3	21	theme	viscoelastic	457:468	arg1	behavior					470:477	their viscoelastic behavior	451:477	their viscoelastic behavior	451:477	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	0	22	theme	Water-Templated	0:14	arg1	Microarchitectures					54:71	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures	0:71	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.	0:111	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.					
32255604	4	23	theme	glioblastoma	867:878	arg1	cells					880:884	human glioblastoma cells	861:884	human glioblastoma cells	861:884	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	4	24	theme	±	795:795	arg1	μm					799:800	∼65 ± 2 μm	791:800	∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix)	791:938	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	4	25	theme	reproducible	735:746	arg1	spheroids					763:771	reproducible, uniform tumor spheroids	735:771	reproducible, uniform tumor spheroids	735:771	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	0	26	theme	Bioartificial	37:49	arg1	Microarchitectures					54:71	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures	0:71	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.	0:111	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.					
32255604	4	27	theme	Agarose	924:930	arg1	matrix					932:937	Agarose matrix	924:937	Agarose matrix	924:937	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	1	28	theme	first	125:129	arg1	This					113:116	This	113:116	This	113:116	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	1	28	theme	first	125:129	arg1	report					131:136	the first report	121:136	the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM)	121:256	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	5	29	theme	limited	1017:1023	arg1	drugs					1032:1036	limited cancer drugs	1017:1036	limited cancer drugs	1017:1036	Thereafter, their capacity for anticancer drug screening was examined using limited cancer drugs.					
32255604	4	30	dep	μm	854:855	arg1	spheroids					910:918	nonuniform spheroids	899:918	nonuniform spheroids	899:918	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	5	31	theme	anticancer	972:981	arg1	screening					988:996	anticancer drug screening	972:996	anticancer drug screening	972:996	Thereafter, their capacity for anticancer drug screening was examined using limited cancer drugs.					
32255604	6	32	theme	drug	1190:1193	arg1	access					1195:1200	decreased drug access	1180:1200	decreased drug access	1180:1200	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	4	33	theme	μm	799:800	arg1	Z-depth					780:786	a Z-depth	778:786	a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix)	778:938	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	1	34	theme	true	220:223	arg1	ECM					253:255	ECM	253:255	ECM	253:255	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	1	34	theme	true	220:223	arg1	matrix					245:250	a true human extracellular matrix	218:250	a true human extracellular matrix (ECM)	218:256	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	4	35	theme	hybrid	707:712	arg1	composites					714:723	The hybrid composites	703:723	The hybrid composites	703:723	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	4	36	theme	±	850:850	arg1	U-251					887:891	U-251	887:891	U-251	887:891	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	4	36	theme	±	850:850	arg1	μm					854:855	∼54 ± 3 μm	846:855	∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix)	846:938	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	1	37	theme	human	225:229	arg1	ECM					253:255	ECM	253:255	ECM	253:255	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	1	37	theme	human	225:229	arg1	matrix					245:250	a true human extracellular matrix	218:250	a true human extracellular matrix (ECM)	218:256	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	0	38	theme	3D	51:52	arg1	Microarchitectures					54:71	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures	0:71	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.	0:111	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.					
32255604	3	39	theme	origins	694:700	arg1	spheroids					671:679	3D tumor spheroids	662:679	3D tumor spheroids of divergent origins	662:700	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	3	40	theme	3D	662:663	arg1	spheroids					671:679	3D tumor spheroids	662:679	3D tumor spheroids of divergent origins	662:700	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	1	41	theme	extracellular	231:243	arg1	ECM					253:255	ECM	253:255	ECM	253:255	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	1	41	theme	extracellular	231:243	arg1	matrix					245:250	a true human extracellular matrix	218:250	a true human extracellular matrix (ECM)	218:256	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	0	42	theme	Extra-Cellular	76:89	arg1	Bioautomatons					98:110	Extra-Cellular Matrix Bioautomatons	76:110	Extra-Cellular Matrix Bioautomatons	76:110	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.					
32255604	4	43	theme	nonuniform	899:908	arg1	spheroids					910:918	nonuniform spheroids	899:918	nonuniform spheroids	899:918	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	8	44	dep	real-time	1332:1340	arg1	phenotypic					1357:1366	phenotypic	1357:1366	phenotypic	1357:1366	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	8	44	dep	real-time	1332:1340	arg1	high-content					1343:1354	high-content	1343:1354	high-content	1343:1354	Moreover, their potential for real-time, high-content, phenotypic precision oncology was affirmed by the exceptional transparency of the synthesized composite.					
32255604	6	45	theme	glioblastoma	1114:1125	arg1	cells					1127:1131	glioblastoma cells	1114:1131	glioblastoma cells cultured on our 3D matrix	1114:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	2	46	theme	defined	362:368	arg1	3D-microarchitecture					370:389	polysaccharide-rich and chemically defined 3D-microarchitecture	327:389	polysaccharide-rich and chemically defined 3D-microarchitecture	327:389	Herein, water behaved as a quasi-spherical porogen, for engineering polysaccharide-rich and chemically defined 3D-microarchitecture, with semi-interpenetrating networks (S-IPNs).					
32255604	4	47	from	spheroids	910:918	arg1	matrix					932:937	Agarose matrix	924:937	Agarose matrix	924:937	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	3	48	dep	scaffolds	593:601	arg1	permitting					623:632	permitting	623:632	permitting	623:632	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	3	48	dep	scaffolds	593:601	arg1	promoting					638:646	promoting	638:646	promoting the growth of 3D tumor spheroids of divergent origins	638:700	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	0	49	dep	Water-Templated	0:14	arg1	Polysaccharide-rich					17:35	Polysaccharide-rich	17:35	Polysaccharide-rich	17:35	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.					
32255604	1	50	theme	quasi-spherical	157:171	arg1	shape					174:178	the "quasi-spherical" shape	152:178	the "quasi-spherical" shape of water molecules	152:197	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
32255604	6	51	theme	lower	1211:1215	arg1	ingression					1217:1226	lower ingression	1211:1226	lower ingression	1211:1226	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	5	52	theme	cancer	1025:1030	arg1	drugs					1032:1036	limited cancer drugs	1017:1036	limited cancer drugs	1017:1036	Thereafter, their capacity for anticancer drug screening was examined using limited cancer drugs.					
32255604	4	53	theme	∼65	791:793	arg1	μm					799:800	∼65 ± 2 μm	791:800	∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix)	791:938	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	3	54	dep	instructive	543:553	arg1	self-remodeling					556:570	self-remodeling	556:570	self-remodeling	556:570	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	3	55	theme	divergent	684:692	arg1	origins					694:700	divergent origins	684:700	divergent origins	684:700	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	6	56	from	efficacy	1102:1109	arg1	cells					1127:1131	glioblastoma cells	1114:1131	glioblastoma cells cultured on our 3D matrix	1114:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	2	57	theme	polysaccharide-rich	327:345	arg1	3D-microarchitecture					370:389	polysaccharide-rich and chemically defined 3D-microarchitecture	327:389	polysaccharide-rich and chemically defined 3D-microarchitecture	327:389	Herein, water behaved as a quasi-spherical porogen, for engineering polysaccharide-rich and chemically defined 3D-microarchitecture, with semi-interpenetrating networks (S-IPNs).					
32255604	3	58	theme	heterogeneous	492:504	arg1	morphology					519:528	a heterogeneous, fibroporous morphology	490:528	a heterogeneous, fibroporous morphology	490:528	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	3	59	theme	tumor	665:669	arg1	spheroids					671:679	3D tumor spheroids	662:679	3D tumor spheroids of divergent origins	662:700	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	6	60	theme	screening	1060:1068	arg1	results					1070:1076	The conflicting drug screening results	1039:1076	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix	1039:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	2	61	theme	semi-interpenetrating	397:417	arg1	S-IPNs					429:434	S-IPNs	429:434	S-IPNs	429:434	Herein, water behaved as a quasi-spherical porogen, for engineering polysaccharide-rich and chemically defined 3D-microarchitecture, with semi-interpenetrating networks (S-IPNs).					
32255604	2	61	theme	semi-interpenetrating	397:417	arg1	networks					419:426	semi-interpenetrating networks	397:426	semi-interpenetrating networks (S-IPNs)	397:435	Herein, water behaved as a quasi-spherical porogen, for engineering polysaccharide-rich and chemically defined 3D-microarchitecture, with semi-interpenetrating networks (S-IPNs).					
32255604	9	62	theme	3D	1473:1474	arg1	microarchitecture					1476:1492	this 3D microarchitecture	1468:1492	this 3D microarchitecture	1468:1492	Since this 3D microarchitecture typifies ECM bioautomaton, this matrix can also be wielded for precision oncology.					
32255604	0	63	theme	Matrix	91:96	arg1	Bioautomatons					98:110	Extra-Cellular Matrix Bioautomatons	76:110	Extra-Cellular Matrix Bioautomatons	76:110	Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons.					
32255604	6	64	theme	3D	1149:1150	arg1	matrix					1152:1157	our 3D matrix	1145:1157	our 3D matrix	1145:1157	The conflicting drug screening results for Etoposide's reduced efficacy on glioblastoma cells cultured on our 3D matrix could be ascribed to decreased drug access and thus lower ingression.					
32255604	3	65	theme	spheroids	671:679	arg1	growth					652:657	the growth	648:657	the growth of 3D tumor spheroids of divergent origins	648:700	Furthermore, their viscoelastic behavior along with a heterogeneous, fibroporous morphology, facilitated instructive, self-remodeling of the bioartificial scaffolds, thence effectively permitting and promoting the growth of 3D tumor spheroids of divergent origins.					
32255604	4	66	dep	reproducible	735:746	arg1	uniform					749:755	uniform	749:755	uniform	749:755	The hybrid composites displayed reproducible, uniform tumor spheroids with a Z-depth of ∼65 ± 2 μm in case of human adenocarcinoma (DLD-1) and ∼54 ± 3 μm for human glioblastoma cells (U-251) (vs. nonuniform spheroids, on Agarose matrix).					
32255604	1	67	theme	"	172:172	arg1	shape					174:178	the "quasi-spherical" shape	152:178	the "quasi-spherical" shape of water molecules	152:197	This is the first report of exploiting the "quasi-spherical" shape of water molecules for recapitulating a true human extracellular matrix (ECM).					
33119268	0	0	theme	Nanoemulsions	121:133	arg1	Deconstitution					103:116	Extremely Efficient Deconstitution	83:116	Extremely Efficient Deconstitution of Nanoemulsions	83:133	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	4	1	theme	oil-in-water	693:704	arg1	micro/nanoemulsions					706:724	surfactant-stabilized oil-in-water micro/nanoemulsions	671:724	surfactant-stabilized oil-in-water micro/nanoemulsions	671:724	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	4	2	theme	versatile	606:614	arg1	membrane					628:635	this versatile antifouling membrane	601:635	this versatile antifouling membrane	601:635	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	5	3	theme	soft	943:946	arg1	approach					958:965	a naturally occurring soft materials approach	921:965	a naturally occurring soft materials approach with lubricious conformal interfaces	921:1002	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	4	4	theme	surfactant-stabilized	671:691	arg1	micro/nanoemulsions					706:724	surfactant-stabilized oil-in-water micro/nanoemulsions	671:724	surfactant-stabilized oil-in-water micro/nanoemulsions	671:724	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	3	5	theme	water-infused	454:466	arg1	membrane					494:501	a green water-infused superoleophobic composite membrane	446:501	a green water-infused superoleophobic composite membrane	446:501	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	2	6	from	exploration	374:384	arg1	nature					418:423	nature	418:423	nature	418:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	4	7	theme	oil	751:753	arg1	fraction					755:762	the oil fraction	747:762	the oil fraction with very high filtration efficiency	747:799	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	1	8	theme	critical	167:174	arg1	properties					188:197	the most critical interfacial properties	158:197	the most critical interfacial properties of any surface	158:212	Wettability is one of the most critical interfacial properties of any surface.					
33119268	2	9	from	phenomena	405:413	arg1	nature					418:423	nature	418:423	nature	418:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	2	10	theme	wide-ranging	331:342	arg1	applicability					344:356	their wide-ranging applicability	325:356	their wide-ranging applicability	325:356	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	1	11	theme	surface	206:212	arg1	properties					188:197	the most critical interfacial properties	158:197	the most critical interfacial properties of any surface	158:212	Wettability is one of the most critical interfacial properties of any surface.					
33119268	5	12	theme	materials	948:956	arg1	approach					958:965	a naturally occurring soft materials approach	921:965	a naturally occurring soft materials approach with lubricious conformal interfaces	921:1002	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	2	13	theme	unusual	389:395	arg1	phenomena					405:413	unusual wetting phenomena	389:413	unusual wetting phenomena in nature	389:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	4	14	theme	high	774:777	arg1	efficiency					790:799	very high filtration efficiency	769:799	very high filtration efficiency	769:799	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	0	15	theme	Superoleophobic	11:25	arg1	Biomembranes					66:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes	0:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.	0:134	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	2	16	from	nature	418:423	arg1	exploration					374:384	a biomimetic exploration	361:384	a biomimetic exploration of unusual wetting phenomena in nature	361:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	5	17	theme	suitable	1036:1043	arg1	surfaces					1045:1052	suitable surfaces	1036:1052	suitable surfaces	1036:1052	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	0	18	theme	Underwater	0:9	arg1	Biomembranes					66:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes	0:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.	0:134	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	4	19	theme	filtration	779:788	arg1	efficiency					790:799	very high filtration efficiency	769:799	very high filtration efficiency	769:799	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	3	20	theme	bacteria	515:522	arg1	growth					538:543	bacteria nanocellulose growth	515:543	bacteria nanocellulose growth	515:543	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	0	21	theme	Liquid-Infused	44:57	arg1	Biomembranes					66:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes	0:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.	0:134	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	2	22	theme	biomimetic	363:372	arg1	exploration					374:384	a biomimetic exploration	361:384	a biomimetic exploration of unusual wetting phenomena in nature	361:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	3	23	theme	reinforcement	550:562	arg1	substrate					572:580	a reinforcement fibrous substrate	548:580	a reinforcement fibrous substrate	548:580	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	0	24	theme	Matrix-Formatted	27:42	arg1	Biomembranes					66:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes	0:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.	0:134	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	5	25	theme	bacteria	834:841	arg1	matrices					857:864	bacteria nanocellulose matrices	834:864	bacteria nanocellulose matrices	834:864	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	3	26	theme	fibrous	564:570	arg1	substrate					572:580	a reinforcement fibrous substrate	548:580	a reinforcement fibrous substrate	548:580	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	5	27	theme	nanocellulose	843:855	arg1	matrices					857:864	bacteria nanocellulose matrices	834:864	bacteria nanocellulose matrices	834:864	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	0	28	theme	Porous	59:64	arg1	Biomembranes					66:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes	0:77	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.	0:134	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	5	29	theme	occurring	933:941	arg1	approach					958:965	a naturally occurring soft materials approach	921:965	a naturally occurring soft materials approach with lubricious conformal interfaces	921:1002	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	5	30	theme	lubricious	972:981	arg1	interfaces					993:1002	lubricious conformal interfaces	972:1002	lubricious conformal interfaces	972:1002	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	5	31	theme	current	885:891	arg1	technologies					893:904	current technologies	885:904	current technologies	885:904	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	3	32	theme	superoleophobic	468:482	arg1	membrane					494:501	a green water-infused superoleophobic composite membrane	446:501	a green water-infused superoleophobic composite membrane	446:501	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	4	33	theme	antifouling	616:626	arg1	membrane					628:635	this versatile antifouling membrane	601:635	this versatile antifouling membrane	601:635	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	3	34	theme	nanocellulose	524:536	arg1	growth					538:543	bacteria nanocellulose growth	515:543	bacteria nanocellulose growth	515:543	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	3	35	theme	composite	484:492	arg1	membrane					494:501	a green water-infused superoleophobic composite membrane	446:501	a green water-infused superoleophobic composite membrane	446:501	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	5	36	theme	renewable	806:814	arg1	based					825:829	The renewable membrane based	802:829	The renewable membrane based on bacteria nanocellulose matrices	802:864	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	2	37	theme	phenomena	405:413	arg1	exploration					374:384	a biomimetic exploration	361:384	a biomimetic exploration of unusual wetting phenomena in nature	361:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	5	38	theme	membrane	816:823	arg1	based					825:829	The renewable membrane based	802:829	The renewable membrane based on bacteria nanocellulose matrices	802:864	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	2	39	theme	wetting	397:403	arg1	phenomena					405:413	unusual wetting phenomena	389:413	unusual wetting phenomena in nature	389:423	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	5	40	with	approach	958:965	arg1	interfaces					993:1002	lubricious conformal interfaces	972:1002	lubricious conformal interfaces	972:1002	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	4	41	with	fraction	755:762	arg1	efficiency					790:799	very high filtration efficiency	769:799	very high filtration efficiency	769:799	It was shown that this versatile antifouling membrane is capable of removing water from surfactant-stabilized oil-in-water micro/nanoemulsions and helps to isolate the oil fraction with very high filtration efficiency.					
33119268	2	42	with	Surfaces	215:222	arg1	wettability					237:247	special wettability	229:247	special wettability	229:247	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	5	43	from	based	825:829	arg1	matrices					857:864	bacteria nanocellulose matrices	834:864	bacteria nanocellulose matrices	834:864	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	2	44	theme	special	229:235	arg1	wettability					237:247	special wettability	229:247	special wettability	229:247	Surfaces with special wettability such as superwetting or superantiwetting are being intensively explored for their wide-ranging applicability by a biomimetic exploration of unusual wetting phenomena in nature.					
33119268	1	45	theme	interfacial	176:186	arg1	properties					188:197	the most critical interfacial properties	158:197	the most critical interfacial properties of any surface	158:212	Wettability is one of the most critical interfacial properties of any surface.					
33119268	3	46	theme	green	448:452	arg1	membrane					494:501	a green water-infused superoleophobic composite membrane	446:501	a green water-infused superoleophobic composite membrane	446:501	This study provides a green water-infused superoleophobic composite membrane by boosting bacteria nanocellulose growth on a reinforcement fibrous substrate.					
33119268	0	47	theme	Efficient	93:101	arg1	Deconstitution					103:116	Extremely Efficient Deconstitution	83:116	Extremely Efficient Deconstitution of Nanoemulsions	83:133	Underwater Superoleophobic Matrix-Formatted Liquid-Infused Porous Biomembranes for Extremely Efficient Deconstitution of Nanoemulsions.					
33119268	5	48	theme	conformal	983:991	arg1	interfaces					993:1002	lubricious conformal interfaces	972:1002	lubricious conformal interfaces	972:1002	The renewable membrane based on bacteria nanocellulose matrices can vastly improve current technologies by cultivating a naturally occurring soft materials approach with lubricious conformal interfaces to effectively and simply cover suitable surfaces.					
33119268	1	49	theme	properties	188:197	arg1	properties					188:197	the most critical interfacial properties	158:197	the most critical interfacial properties of any surface	158:212	Wettability is one of the most critical interfacial properties of any surface.					
33119268	1	49	theme	properties	188:197	arg1	one					151:153	one	151:153	one	151:153	Wettability is one of the most critical interfacial properties of any surface.					
33033395	10	0	from	EI	1692:1693	arg1	P < 0.01					1733:1740	P < 0.01	1733:1740	P < 0.01	1733:1740	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	10	0	from	EI	1692:1693	arg1	females					1724:1730	females	1724:1730	females (P < 0.01)	1724:1741	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	13	1	theme	gut	2317:2319	arg1	composition					2332:2342	gut microbiota composition	2317:2342	gut microbiota composition	2317:2342	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	10	2	theme	Ob	1564:1565	arg1	challenge					1567:1575	The Ob challenge	1560:1575	The Ob challenge	1560:1575	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	9	3	dep	test	1485:1488	arg1	P < 0.05					1522:1529	P < 0.05	1522:1529	P < 0.05	1522:1529	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	9	3	dep	test	1485:1488	arg1	P < 0.001					1506:1514	peak [Glucose] P < 0.001	1491:1514	peak [Glucose] P < 0.001	1491:1514	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	10	4	theme	greater	1589:1595	arg1	gain					1600:1603	greater BW gain	1589:1603	greater BW gain in both male and female OffOb versus OffCon (P < 0.05)	1589:1658	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	1	5	theme	maternal	183:190	arg1	diet-induced-obesity					192:211	maternal diet-induced-obesity	183:211	maternal diet-induced-obesity	183:211	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	10	6	from	EE	1718:1719	arg1	P < 0.01					1733:1740	P < 0.01	1733:1740	P < 0.01	1733:1740	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	10	6	from	EE	1718:1719	arg1	females					1724:1730	females	1724:1730	females (P < 0.01)	1724:1741	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	13	7	dep	CONCLUSIONS	2134:2144	arg1	influences					2173:2182	influences	2173:2182	influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function	2173:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	13	8	from	changes	2306:2312	arg1	muscle					2357:2362	skeletal muscle	2348:2362	skeletal muscle	2348:2362	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	13	8	from	changes	2306:2312	arg1	composition					2332:2342	gut microbiota composition	2317:2342	gut microbiota composition	2317:2342	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	11	9	theme	night-time	1856:1865	arg1	EE					1867:1868	increased night-time EE	1846:1868	increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001)	1846:1922	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	8	10	theme	male	1189:1192	arg1	OffOb					1194:1198	Six-month-old male OffOb	1175:1198	Six-month-old male OffOb	1175:1198	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	12	11	theme	muscle	1968:1973	arg1	number					1986:1991	skeletal muscle mtDNA copy number	1959:1991	skeletal muscle mtDNA copy number	1959:1991	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	1	12	theme	maternal	406:413	arg1	obesity					415:421	maternal obesity	406:421	maternal obesity	406:421	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	8	13	theme	increased	1213:1221	arg1	P < 0.05					1281:1288	P < 0.05	1281:1288	P < 0.05	1281:1288	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	13	theme	increased	1213:1221	arg1	mass					1275:1278	increased bodyweight (BW, P < 0.001) and white adipose tissue mass	1213:1278	increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05)	1213:1289	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	13	14	theme	PDX	2276:2278	arg1	treatment					2280:2288	maternal PDX treatment	2267:2288	maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function	2267:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	11	15	theme	male	1878:1881	arg1	P < 0.001					1913:1921	P < 0.001	1913:1921	P < 0.001	1913:1921	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	11	15	theme	male	1878:1881	arg1	OffObP					1905:1910	both male (P < 0.05) and female OffObP	1873:1910	both male (P < 0.05) and female OffObP (P < 0.001)	1873:1922	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	12	16	theme	male	2100:2103	arg1	OffObP					2105:2110	male OffObP	2100:2110	male OffObP relative to controls	2100:2131	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	8	17	theme	bodyweight	1223:1232	arg1	P < 0.05					1281:1288	P < 0.05	1281:1288	P < 0.05	1281:1288	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	17	theme	bodyweight	1223:1232	arg1	mass					1275:1278	increased bodyweight (BW, P < 0.001) and white adipose tissue mass	1213:1278	increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05)	1213:1289	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	1	18	from	susceptibility	275:288	arg1	adulthood					304:312	adulthood	304:312	adulthood	304:312	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	3	19	theme	obese	570:574	arg1	dams					576:579	the obese dams	566:579	Half the obese dams	561:579	Half the obese dams were supplemented with 5% PDX (ObPDX) in drinking water throughout pregnancy and lactation.					
33033395	5	20	theme	glucose-tolerance	836:852	arg1	test					854:857	a glucose-tolerance test	834:857	a glucose-tolerance test	834:857	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	8	21	theme	night-time	1351:1360	arg1	EE					1362:1363	lower night-time EE	1345:1363	lower night-time EE (P < 0.001)	1345:1375	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	21	theme	night-time	1351:1360	arg1	P < 0.001					1366:1374	P < 0.001	1366:1374	P < 0.001	1366:1374	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	1	22	from	expenditure	259:269	arg1	adulthood					304:312	adulthood	304:312	adulthood	304:312	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	3	23	theme	%	605:605	arg1	ObPDX					612:616	ObPDX	612:616	ObPDX	612:616	Half the obese dams were supplemented with 5% PDX (ObPDX) in drinking water throughout pregnancy and lactation.					
33033395	3	23	theme	%	605:605	arg1	PDX					607:609	5% PDX	604:609	5% PDX (ObPDX)	604:617	Half the obese dams were supplemented with 5% PDX (ObPDX) in drinking water throughout pregnancy and lactation.					
33033395	8	24	dep	mass	1323:1326	arg1	P < 0.01					1334:1341	P < 0.01	1334:1341	P < 0.01	1334:1341	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	24	dep	mass	1323:1326	arg1	BAT					1329:1331	BAT	1329:1331	BAT	1329:1331	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	0	25	theme	obese	51:55	arg1	pregnancy					63:71	obese mouse pregnancy	51:71	obese mouse pregnancy	51:71	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	11	26	theme	BW	1783:1784	arg1	gain					1786:1789	accelerated BW gain	1771:1789	accelerated BW gain on the OB diet	1771:1804	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	7	27	theme	mitochondrial	1123:1135	arg1	activity					1137:1144	mitochondrial activity	1123:1144	mitochondrial activity	1123:1144	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	3	28	dep	Half	561:564	arg1	dams					576:579	the obese dams	566:579	Half the obese dams	561:579	Half the obese dams were supplemented with 5% PDX (ObPDX) in drinking water throughout pregnancy and lactation.					
33033395	0	29	from	Supplementation	0:14	arg1	pregnancy					63:71	obese mouse pregnancy	51:71	obese mouse pregnancy	51:71	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	8	30	theme	adipose	1260:1266	arg1	tissue					1268:1273	white adipose tissue	1254:1273	white adipose tissue	1254:1273	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	12	31	theme	copy	1981:1984	arg1	number					1986:1991	skeletal muscle mtDNA copy number	1959:1991	skeletal muscle mtDNA copy number	1959:1991	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	0	32	theme	maternal	82:89	arg1	homeostasis					99:109	maternal glucose homeostasis	82:109	maternal glucose homeostasis	82:109	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	6	33	theme	obese	901:905	arg1	Offspring					870:878	Offspring	870:878	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams	870:948	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams were subsequently challenged for 3 weeks with Ob, and energy balanced reassessed.					
33033395	2	34	theme	METHODS	424:430	arg1	mice					439:442	METHODS Female mice	424:442	METHODS Female mice	424:442	METHODS Female mice were fed a control (Con) or obesogenic diet (Ob) for 6 weeks prior to mating and throughout pregnancy and lactation.					
33033395	1	35	theme	programming	391:401	arg1	consequences					375:386	the consequences	371:386	the consequences of programming	371:401	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	0	36	theme	mouse	57:61	arg1	pregnancy					63:71	obese mouse pregnancy	51:71	obese mouse pregnancy	51:71	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	12	37	theme	mtDNA	1975:1979	arg1	number					1986:1991	skeletal muscle mtDNA copy number	1959:1991	skeletal muscle mtDNA copy number	1959:1991	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	13	38	theme	adult	2184:2188	arg1	balance					2207:2213	adult offspring energy balance	2184:2213	adult offspring energy balance	2184:2213	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	7	39	theme	energy	1065:1070	arg1	balance					1072:1078	offspring energy balance	1055:1078	offspring energy balance	1055:1078	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	13	40	theme	energy	2200:2205	arg1	balance					2207:2213	adult offspring energy balance	2184:2213	adult offspring energy balance	2184:2213	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	10	41	theme	increased	1682:1690	arg1	P < 0.05					1696:1703	P < 0.05	1696:1703	P < 0.05	1696:1703	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	10	41	theme	increased	1682:1690	arg1	EI					1692:1693	EI	1692:1693	EI (P < 0.05)	1692:1704	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	8	42	theme	brown	1302:1306	arg1	mass					1323:1326	decreased brown adipose tissue mass	1292:1326	decreased brown adipose tissue mass (BAT, P < 0.01)	1292:1342	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	5	43	theme	offspring	734:742	arg1	EI					759:760	EI	759:760	EI	759:760	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	5	43	theme	offspring	734:742	arg1	intake					751:756	offspring energy intake	734:756	offspring energy intake (EI)	734:761	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	1	44	theme	prebiotic	328:336	arg1	polydextrose					338:349	the prebiotic polydextrose	324:349	the prebiotic polydextrose (PDX)	324:355	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	1	44	theme	prebiotic	328:336	arg1	PDX					352:354	PDX	352:354	PDX	352:354	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	13	45	theme	skeletal	2348:2355	arg1	muscle					2357:2362	skeletal muscle	2348:2362	skeletal muscle	2348:2362	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	9	46	theme	glucose-tolerance	1467:1483	arg1	test					1485:1488	abnormal glucose-tolerance test	1458:1488	abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX	1458:1557	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	8	47	theme	tissue	1316:1321	arg1	mass					1323:1326	decreased brown adipose tissue mass	1292:1326	decreased brown adipose tissue mass (BAT, P < 0.01)	1292:1342	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	7	48	theme	Potential	1032:1040	arg1	modifiers					1042:1050	Potential modifiers	1032:1050	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity	1032:1144	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	7	48	theme	Potential	1032:1040	arg1	biomarkers					1109:1118	biomarkers	1109:1118	biomarkers of mitochondrial activity	1109:1144	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	7	48	theme	Potential	1032:1040	arg1	microbiota					1094:1103	gut microbiota	1090:1103	gut microbiota	1090:1103	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	0	49	with	Supplementation	0:14	arg1	polydextrose					34:45	polydextrose	34:45	polydextrose	34:45	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	0	49	with	Supplementation	0:14	arg1	prebiotic					23:31	a prebiotic	21:31	a prebiotic (polydextrose)	21:46	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	13	50	with	treatment	2280:2288	arg1	changes					2306:2312	associated changes	2295:2312	associated changes in gut microbiota composition and skeletal muscle mitochondrial function	2295:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	1	51	theme	energy	252:257	arg1	expenditure					259:269	energy expenditure	252:269	energy expenditure	252:269	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	11	52	theme	OB	1798:1799	arg1	diet					1801:1804	the OB diet	1794:1804	the OB diet	1794:1804	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	9	53	theme	[Glucose	1496:1503	arg1	P < 0.05					1522:1529	P < 0.05	1522:1529	P < 0.05	1522:1529	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	9	53	theme	[Glucose	1496:1503	arg1	P < 0.001					1506:1514	peak [Glucose] P < 0.001	1491:1514	peak [Glucose] P < 0.001	1491:1514	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	6	54	dep	reassessed	1020:1029	arg1	balanced					1011:1018	balanced	1011:1018	balanced	1011:1018	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams were subsequently challenged for 3 weeks with Ob, and energy balanced reassessed.					
33033395	5	55	theme	indirect	793:800	arg1	calorimetry					802:812	indirect calorimetry	793:812	indirect calorimetry	793:812	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	11	56	theme	female	1898:1903	arg1	P < 0.001					1913:1921	P < 0.001	1913:1921	P < 0.001	1913:1921	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	11	56	theme	female	1898:1903	arg1	OffObP					1905:1910	both male (P < 0.05) and female OffObP	1873:1910	both male (P < 0.05) and female OffObP (P < 0.001)	1873:1922	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	12	57	theme	relative	2112:2119	arg1	OffObP					2105:2110	male OffObP	2100:2110	male OffObP relative to controls	2100:2131	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	10	58	theme	reduced	1710:1716	arg1	EE					1718:1719	reduced EE	1710:1719	reduced EE in females (P < 0.01)	1710:1741	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	13	59	theme	microbiota	2321:2330	arg1	composition					2332:2342	gut microbiota composition	2317:2342	gut microbiota composition	2317:2342	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	12	60	theme	skeletal	1959:1966	arg1	number					1986:1991	skeletal muscle mtDNA copy number	1959:1991	skeletal muscle mtDNA copy number	1959:1991	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	11	61	dep	male	1878:1881	arg1	P < 0.05					1884:1891	P < 0.05	1884:1891	P < 0.05	1884:1891	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	10	62	theme	BW	1597:1598	arg1	gain					1600:1603	greater BW gain	1589:1603	greater BW gain in both male and female OffOb versus OffCon (P < 0.05)	1589:1658	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	13	63	theme	mitochondrial	2364:2376	arg1	function					2378:2385	mitochondrial function	2364:2385	mitochondrial function	2364:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	11	64	from	gain	1786:1789	arg1	diet					1801:1804	the OB diet	1794:1804	the OB diet	1794:1804	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	13	65	theme	associated	2295:2304	arg1	changes					2306:2312	associated changes	2295:2312	associated changes in gut microbiota composition and skeletal muscle mitochondrial function	2295:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	11	66	dep	protected	1756:1764	arg1	associated					1830:1839	associated	1830:1839	associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001)	1830:1922	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	1	67	theme	adverse	217:223	arg1	consequences					225:236	adverse consequences	217:236	adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood	217:312	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	9	68	dep	P < 0.001	1506:1514	arg1	AUC					1517:1519	AUC	1517:1519	AUC	1517:1519	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	13	69	theme	maternal	2267:2274	arg1	treatment					2280:2288	maternal PDX treatment	2267:2288	maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function	2267:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	6	70	theme	control	883:889	arg1	Offspring					870:878	Offspring	870:878	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams	870:948	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams were subsequently challenged for 3 weeks with Ob, and energy balanced reassessed.					
33033395	11	71	theme	increased	1846:1854	arg1	EE					1867:1868	increased night-time EE	1846:1868	increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001)	1846:1922	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	8	72	theme	lower	1345:1349	arg1	EE					1362:1363	lower night-time EE	1345:1363	lower night-time EE (P < 0.001)	1345:1375	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	72	theme	lower	1345:1349	arg1	P < 0.001					1366:1374	P < 0.001	1366:1374	P < 0.001	1366:1374	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	12	73	theme	cells	2071:2075	arg1	percentage					2045:2054	the percentage	2041:2054	the percentage of Bacteroides cells	2041:2075	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	9	74	dep	male	1429:1432	arg1	OffOb					1445:1449	OffOb	1445:1449	OffOb	1445:1449	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	3	75	theme	5	604:604	arg1	%					605:605	%	605:605	%	605:605	Half the obese dams were supplemented with 5% PDX (ObPDX) in drinking water throughout pregnancy and lactation.					
33033395	12	76	theme	faecal	2080:2085	arg1	samples					2087:2093	faecal samples	2080:2093	faecal samples from male OffObP relative to controls	2080:2131	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	7	77	theme	activity	1137:1144	arg1	biomarkers					1109:1118	biomarkers	1109:1118	biomarkers of mitochondrial activity	1109:1144	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	7	77	theme	activity	1137:1144	arg1	microbiota					1094:1103	gut microbiota	1090:1103	gut microbiota	1090:1103	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	11	78	theme	accelerated	1771:1781	arg1	gain					1786:1789	accelerated BW gain	1771:1789	accelerated BW gain on the OB diet	1771:1804	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	5	79	theme	energy	767:772	arg1	EE					787:788	EE	787:788	EE	787:788	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	5	79	theme	energy	767:772	arg1	expenditure					774:784	energy expenditure	767:784	energy expenditure (EE by indirect calorimetry)	767:813	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	10	80	from	gain	1600:1603	arg1	OffOb					1629:1633	OffOb	1629:1633	OffOb	1629:1633	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	10	80	from	gain	1600:1603	arg1	P < 0.05					1650:1657	P < 0.05	1650:1657	P < 0.05	1650:1657	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	6	81	theme	PDX	919:921	arg1	Offspring					870:878	Offspring	870:878	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams	870:948	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams were subsequently challenged for 3 weeks with Ob, and energy balanced reassessed.					
33033395	0	82	theme	glucose	91:97	arg1	homeostasis					99:109	maternal glucose homeostasis	82:109	maternal glucose homeostasis	82:109	Supplementation with a prebiotic (polydextrose) in obese mouse pregnancy improves maternal glucose homeostasis and protects against offspring obesity.					
33033395	2	83	theme	prior	505:509	arg1	weeks					499:503	6 weeks	497:503	6 weeks prior to mating	497:519	METHODS Female mice were fed a control (Con) or obesogenic diet (Ob) for 6 weeks prior to mating and throughout pregnancy and lactation.					
33033395	8	84	theme	white	1254:1258	arg1	tissue					1268:1273	white adipose tissue	1254:1273	white adipose tissue	1254:1273	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	11	85	from	EE	1867:1868	arg1	P < 0.001					1913:1921	P < 0.001	1913:1921	P < 0.001	1913:1921	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	11	85	from	EE	1867:1868	arg1	OffObP					1905:1910	both male (P < 0.05) and female OffObP	1873:1910	both male (P < 0.05) and female OffObP (P < 0.001)	1873:1922	OffObP were protected from accelerated BW gain on the OB diet compared with controls, associated with increased night-time EE in both male (P < 0.05) and female OffObP (P < 0.001).					
33033395	7	86	theme	gut	1090:1092	arg1	microbiota					1094:1103	gut microbiota	1090:1103	gut microbiota	1090:1103	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	8	87	theme	Six-month-old	1175:1187	arg1	OffOb					1194:1198	Six-month-old male OffOb	1175:1198	Six-month-old male OffOb	1175:1198	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	88	theme	tissue	1268:1273	arg1	P < 0.05					1281:1288	P < 0.05	1281:1288	P < 0.05	1281:1288	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	88	theme	tissue	1268:1273	arg1	mass					1275:1278	increased bodyweight (BW, P < 0.001) and white adipose tissue mass	1213:1278	increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05)	1213:1289	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	7	89	theme	balance	1072:1078	arg1	modifiers					1042:1050	Potential modifiers	1032:1050	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity	1032:1144	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	7	89	theme	balance	1072:1078	arg1	biomarkers					1109:1118	biomarkers	1109:1118	biomarkers of mitochondrial activity	1109:1144	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	7	89	theme	balance	1072:1078	arg1	microbiota					1094:1103	gut microbiota	1090:1103	gut microbiota	1090:1103	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	8	90	dep	RESULTS	1167:1173	arg1	demonstrated					1200:1211	demonstrated	1200:1211	demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP	1200:1421	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	12	91	theme	Bacteroides	2059:2069	arg1	cells					2071:2075	Bacteroides cells	2059:2075	Bacteroides cells	2059:2075	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	13	92	dep	composition	2332:2342	arg1	function					2378:2385	mitochondrial function	2364:2385	mitochondrial function	2364:2385	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	4	93	theme	standard	700:707	arg1	chow					709:712	standard chow	700:712	standard chow	700:712	Offspring were weaned onto standard chow.					
33033395	7	94	theme	offspring	1055:1063	arg1	balance					1072:1078	offspring energy balance	1055:1078	offspring energy balance	1055:1078	Potential modifiers of offspring energy balance including gut microbiota and biomarkers of mitochondrial activity were also evaluated.					
33033395	13	95	theme	offspring	2190:2198	arg1	balance					2207:2213	adult offspring energy balance	2184:2213	adult offspring energy balance	2184:2213	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	1	96	contain	has	213:215	arg1	diet-induced-obesity					192:211	maternal diet-induced-obesity	183:211	maternal diet-induced-obesity	183:211	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	1	96	contain	has	213:215	arg2	consequences					225:236	adverse consequences	217:236	adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood	217:312	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	1	97	dep	OBJECTIVES	151:160	arg1	hypothesised					165:176	hypothesised	165:176	hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity	165:421	OBJECTIVES We hypothesised that maternal diet-induced-obesity has adverse consequences for offspring energy expenditure and susceptibility to obesity in adulthood, and that the prebiotic polydextrose (PDX) would prevent the consequences of programming by maternal obesity.					
33033395	9	98	theme	abnormal	1458:1465	arg1	test					1485:1488	abnormal glucose-tolerance test	1458:1488	abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX	1458:1557	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	2	99	theme	obesogenic	472:481	arg1	Ob					489:490	Ob	489:490	Ob	489:490	METHODS Female mice were fed a control (Con) or obesogenic diet (Ob) for 6 weeks prior to mating and throughout pregnancy and lactation.					
33033395	2	99	theme	obesogenic	472:481	arg1	diet					483:486	obesogenic diet	472:486	obesogenic diet (Ob)	472:491	METHODS Female mice were fed a control (Con) or obesogenic diet (Ob) for 6 weeks prior to mating and throughout pregnancy and lactation.					
33033395	13	100	theme	Maternal	2146:2153	arg1	obesity					2155:2161	Maternal obesity	2146:2161	Maternal obesity	2146:2161	CONCLUSIONS Maternal obesity adversely influences adult offspring energy balance and propensity for obesity, which is ameliorated by maternal PDX treatment with associated changes in gut microbiota composition and skeletal muscle mitochondrial function.					
33033395	8	101	theme	decreased	1292:1300	arg1	mass					1323:1326	decreased brown adipose tissue mass	1292:1326	decreased brown adipose tissue mass (BAT, P < 0.01)	1292:1342	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	2	102	theme	Female	432:437	arg1	mice					439:442	METHODS Female mice	424:442	METHODS Female mice	424:442	METHODS Female mice were fed a control (Con) or obesogenic diet (Ob) for 6 weeks prior to mating and throughout pregnancy and lactation.					
33033395	5	103	theme	energy	744:749	arg1	EI					759:760	EI	759:760	EI	759:760	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	5	103	theme	energy	744:749	arg1	intake					751:756	offspring energy intake	734:756	offspring energy intake (EI)	734:761	At 3 and 6 months, offspring energy intake (EI) and energy expenditure (EE by indirect calorimetry) were measured, and a glucose-tolerance test performed.					
33033395	6	104	theme	reassessed	1020:1029	arg1	energy					1004:1009	energy	1004:1009	energy balanced reassessed	1004:1029	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams were subsequently challenged for 3 weeks with Ob, and energy balanced reassessed.					
33033395	8	105	theme	adipose	1308:1314	arg1	mass					1323:1326	decreased brown adipose tissue mass	1292:1326	decreased brown adipose tissue mass (BAT, P < 0.01)	1292:1342	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	6	106	dep	control	883:889	arg1	dams					945:948	dams	945:948	dams	945:948	Offspring of control (OffCon), obese (OffOb) and PDX supplemented (OffObP) dams were subsequently challenged for 3 weeks with Ob, and energy balanced reassessed.					
33033395	9	107	theme	peak	1491:1494	arg1	P < 0.05					1522:1529	P < 0.05	1522:1529	P < 0.05	1522:1529	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	9	107	theme	peak	1491:1494	arg1	P < 0.001					1506:1514	peak [Glucose] P < 0.001	1491:1514	peak [Glucose] P < 0.001	1491:1514	Both male and female OffOb showed abnormal glucose-tolerance test (peak [Glucose] P < 0.001; AUC, P < 0.05) which was prevented by PDX.					
33033395	10	108	dep	resulted	1577:1584	arg1	associated					1666:1675	associated	1666:1675	associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01)	1666:1741	The Ob challenge resulted in greater BW gain in both male and female OffOb versus OffCon (P < 0.05), also associated with increased EI (P < 0.05) and reduced EE in females (P < 0.01).					
33033395	12	109	from	increase	1947:1954	arg1	number					1986:1991	skeletal muscle mtDNA copy number	1959:1991	skeletal muscle mtDNA copy number	1959:1991	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33033395	8	110	dep	bodyweight	1223:1232	arg1	P < 0.001					1239:1247	P < 0.001	1239:1247	P < 0.001	1239:1247	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	8	110	dep	bodyweight	1223:1232	arg1	BW					1235:1236	BW	1235:1236	BW	1235:1236	RESULTS Six-month-old male OffOb demonstrated increased bodyweight (BW, P < 0.001) and white adipose tissue mass (P < 0.05), decreased brown adipose tissue mass (BAT, P < 0.01), lower night-time EE (P < 0.001) versus OffCon, which were prevented in OffObP.					
33033395	12	111	from	OffObP	2105:2110	arg1	samples					2087:2093	faecal samples	2080:2093	faecal samples from male OffObP relative to controls	2080:2131	PDX also prevented an increase in skeletal muscle mtDNA copy number in OffOb versus OffCon (P < 0.01) and increased the percentage of Bacteroides cells in faecal samples from male OffObP relative to controls.					
33863429	10	0	dep	acids	1469:1473	arg1	20:5ω3					1487:1492	20:5ω3	1487:1492	20:5ω3	1487:1492	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	10	0	dep	acids	1469:1473	arg1	18:3ω3					1476:1481	18:3ω3	1476:1481	18:3ω3	1476:1481	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	2	1	theme	high	511:514	arg1	contents					516:523	high contents	511:523	high contents of protein and long-chain poly-unsaturated fatty acids	511:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	7	2	contain	has	1059:1061	arg1	albidus					1051:1057	E. albidus	1048:1057	E. albidus	1048:1057	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	7	2	contain	has	1059:1061	arg2	range					1087:1091	a broad thermal optimum range	1063:1091	a broad thermal optimum range	1063:1091	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	1	3	theme	higher	235:240	arg1	survival					242:249	higher survival	235:249	higher survival	235:249	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	2	4	theme	poly-unsaturated	551:566	arg1	acids					574:578	long-chain poly-unsaturated fatty acids	540:578	long-chain poly-unsaturated fatty acids	540:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	10	5	theme	acids	1469:1473	arg1	abundance					1442:1450	the abundance	1438:1450	the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3)	1438:1493	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	10	5	theme	acids	1469:1473	arg1	largest					1499:1505	largest	1499:1505	largest	1499:1505	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	0	6	theme	Thermal	0:6	arg1	optimum					8:14	Thermal optimum	0:14	Thermal optimum for mass production of the live feed organism Enchytraeus albidus	0:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	1	7	theme	species	152:158	arg1	stages					132:137	the larval stages	121:137	the larval stages of many fish species grown in aquaculture	121:179	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	4	8	theme	same	768:771	arg1	number					773:778	approximately the same number	750:778	approximately the same number of cocoons	750:789	At the outset, each microcosm was supplied with approximately the same number of cocoons.					
33863429	11	9	from	cultures	1581:1588	arg1	density					1556:1562	achievable density	1545:1562	achievable density of worms in mass cultures	1545:1588	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	10	10	theme	omega-3	1455:1461	arg1	acids					1469:1473	omega-3 fatty acids	1455:1473	omega-3 fatty acids (18:3ω3 and 20:5ω3)	1455:1493	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	6	11	theme	population	872:881	arg1	growth					883:888	the population growth	868:888	the population growth	868:888	We followed the population growth at seven temperatures in the range of 4-25 °C and investigated body composition in order to find optimal temperature for mass production.					
33863429	2	12	theme	wide	465:468	arg1	range					470:474	a wide range	463:474	a wide range of organic waste materials	463:501	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	8	13	theme	acid	1312:1315	arg1	contents					1317:1324	total fatty acid contents	1300:1324	total fatty acid contents	1300:1324	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	11	14	theme	100 g L-1	1665:1673	arg1	substrate					1675:1683	100 g L-1 substrate	1665:1683	100 g L-1 substrate	1665:1683	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	1	15	theme	juvenile	186:193	arg1	fish					195:198	juvenile fish	186:198	juvenile fish reared on live	186:213	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	3	16	from	effect	619:624	arg1	growth					655:660	population growth	644:660	population growth	644:660	In the present study, we observed the effect of temperature on population growth over five months using soil microcosms.					
33863429	5	17	theme	oats	831:834	arg1	libitum					839:845	rolled oats ad libitum	824:845	rolled oats ad libitum	824:845	Hatched enchytraeids were given rolled oats ad libitum as feed.					
33863429	2	18	theme	waste	487:491	arg1	materials					493:501	organic waste materials	479:501	organic waste materials	479:501	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	11	19	from	density	1556:1562	arg1	cultures					1581:1588	mass cultures	1576:1588	mass cultures	1576:1588	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	8	20	theme	weight	1340:1345	arg1	%					1331:1331	15-20%	1326:1331	15-20% of dry weight	1326:1345	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	8	20	theme	weight	1340:1345	arg1	weight					1340:1345	dry weight	1336:1345	dry weight	1336:1345	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	11	21	theme	mass	1576:1579	arg1	cultures					1581:1588	mass cultures	1576:1588	mass cultures	1576:1588	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	0	22	theme	feed	48:51	arg1	albidus					74:80	the live feed organism Enchytraeus albidus	39:80	the live feed organism Enchytraeus albidus	39:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	9	23	theme	significant	1377:1387	arg1	effect					1389:1394	a highly significant effect	1368:1394	a highly significant effect on fatty acid composition	1368:1420	The temperature had a highly significant effect on fatty acid composition.					
33863429	11	24	from	worms	1567:1571	arg1	cultures					1581:1588	mass cultures	1576:1588	mass cultures	1576:1588	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	11	25	theme	previous	1613:1620	arg1	results					1622:1628	previous results	1613:1628	previous results	1613:1628	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	0	26	theme	Enchytraeus	62:72	arg1	albidus					74:80	the live feed organism Enchytraeus albidus	39:80	the live feed organism Enchytraeus albidus	39:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	12	27	theme	worms	1757:1761	arg1	temperatures					1778:1789	80 g live worms L-1 month-1 at temperatures	1747:1789	80 g live worms L-1 month-1 at temperatures between 15 and 22 °C	1747:1810	Maximum production of biomass can likely reach 80 g live worms L-1 month-1 at temperatures between 15 and 22 °C.					
33863429	1	28	theme	many	142:145	arg1	species					152:158	many fish species	142:158	many fish species grown in aquaculture	142:179	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	12	29	theme	month-1 at	1767:1776	arg1	temperatures					1778:1789	80 g live worms L-1 month-1 at temperatures	1747:1789	80 g live worms L-1 month-1 at temperatures between 15 and 22 °C	1747:1810	Maximum production of biomass can likely reach 80 g live worms L-1 month-1 at temperatures between 15 and 22 °C.					
33863429	9	30	theme	acid	1405:1408	arg1	composition					1410:1420	fatty acid composition	1399:1420	fatty acid composition	1399:1420	The temperature had a highly significant effect on fatty acid composition.					
33863429	2	31	theme	fatty	568:572	arg1	acids					574:578	long-chain poly-unsaturated fatty acids	540:578	long-chain poly-unsaturated fatty acids	540:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	8	32	theme	protein	1241:1247	arg1	contents					1249:1256	protein contents	1241:1256	protein contents	1241:1256	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	8	33	from	contents	1317:1324	arg1	range					1234:1238	this temperature range	1217:1238	this temperature range	1217:1238	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	7	34	theme	optimum	1079:1085	arg1	range					1087:1091	a broad thermal optimum range	1063:1091	a broad thermal optimum range	1063:1091	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	1	35	theme	Live	83:86	arg1	organisms					93:101	Live feed organisms	83:101	Live feed organisms	83:101	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	2	36	theme	sustainable	382:392	arg1	source					394:399	a sustainable source	380:399	a sustainable source of live feed	380:412	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	36	theme	sustainable	382:392	arg1	potential					367:375	potential	367:375	potential	367:375	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	7	37	theme	broad	1065:1069	arg1	range					1087:1091	a broad thermal optimum range	1063:1091	a broad thermal optimum range	1063:1091	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	2	38	dep	produced	444:451	arg1	mass					439:442	mass	439:442	mass	439:442	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	6	39	theme	body	953:956	arg1	composition					958:968	body composition	953:968	body composition in order to find optimal temperature for mass production	953:1025	We followed the population growth at seven temperatures in the range of 4-25 °C and investigated body composition in order to find optimal temperature for mass production.					
33863429	11	40	theme	achievable	1545:1554	arg1	density					1556:1562	achievable density	1545:1562	achievable density of worms in mass cultures	1545:1588	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	8	41	theme	glycogen	1271:1278	arg1	%					1294:1294	glycogen contents 20-25%	1271:1294	glycogen contents 20-25%	1271:1294	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	10	42	theme	low	1510:1512	arg1	temperature					1514:1524	low temperature	1510:1524	low temperature	1510:1524	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	8	43	dep	%	1268:1268	arg1	%					1331:1331	15-20%	1326:1331	15-20% of dry weight	1326:1345	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	8	43	dep	%	1268:1268	arg1	weight					1340:1345	dry weight	1336:1345	dry weight	1336:1345	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	12	44	theme	biomass	1722:1728	arg1	production					1708:1717	Maximum production	1700:1717	Maximum production of biomass	1700:1728	Maximum production of biomass can likely reach 80 g live worms L-1 month-1 at temperatures between 15 and 22 °C.					
33863429	2	45	theme	feed	409:412	arg1	source					394:399	a sustainable source	380:399	a sustainable source of live feed	380:412	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	45	theme	feed	409:412	arg1	potential					367:375	potential	367:375	potential	367:375	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	46	theme	terrestrial	304:314	arg1	albidus					354:360	Enchytraeus albidus	342:360	Enchytraeus albidus	342:360	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	46	theme	terrestrial	304:314	arg1	enchytraeid					316:326	The terrestrial enchytraeid	300:326	The terrestrial enchytraeid (white worm)	300:339	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	46	theme	terrestrial	304:314	arg1	worm					335:338	white worm	329:338	white worm	329:338	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	7	47	theme	E.	1048:1049	arg1	albidus					1051:1057	E. albidus	1048:1057	E. albidus	1048:1057	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	7	48	theme	specific	1170:1177	arg1	rates					1186:1190	specific growth rates	1170:1190	specific growth rates between 6.5 and 6.8%	1170:1211	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	3	49	theme	present	588:594	arg1	study					596:600	the present study	584:600	the present study	584:600	In the present study, we observed the effect of temperature on population growth over five months using soil microcosms.					
33863429	8	50	theme	fatty	1306:1310	arg1	contents					1317:1324	total fatty acid contents	1300:1324	total fatty acid contents	1300:1324	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	7	51	theme	15-22 °C	1156:1163	arg1	range					1147:1151	the range	1143:1151	the range of 15-22 °C	1143:1163	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	7	52	theme	similar	1113:1119	arg1	production					1129:1138	almost similar biomass production	1106:1138	almost similar biomass production	1106:1138	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	9	53	from	effect	1389:1394	arg1	composition					1410:1420	fatty acid composition	1399:1420	fatty acid composition	1399:1420	The temperature had a highly significant effect on fatty acid composition.					
33863429	6	54	theme	optimal	987:993	arg1	temperature					995:1005	optimal temperature	987:1005	optimal temperature for mass production	987:1025	We followed the population growth at seven temperatures in the range of 4-25 °C and investigated body composition in order to find optimal temperature for mass production.					
33863429	4	55	theme	cocoons	783:789	arg1	number					773:778	approximately the same number	750:778	approximately the same number of cocoons	750:789	At the outset, each microcosm was supplied with approximately the same number of cocoons.					
33863429	3	56	theme	population	644:653	arg1	growth					655:660	population growth	644:660	population growth	644:660	In the present study, we observed the effect of temperature on population growth over five months using soil microcosms.					
33863429	1	57	theme	fish	147:150	arg1	species					152:158	many fish species	142:158	many fish species grown in aquaculture	142:179	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	2	58	theme	long-chain	540:549	arg1	acids					574:578	long-chain poly-unsaturated fatty acids	540:578	long-chain poly-unsaturated fatty acids	540:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	10	59	theme	fatty	1463:1467	arg1	acids					1469:1473	omega-3 fatty acids	1455:1473	omega-3 fatty acids (18:3ω3 and 20:5ω3)	1455:1493	In particular, the abundance of omega-3 fatty acids (18:3ω3 and 20:5ω3) was largest at low temperature.					
33863429	3	60	theme	temperature	629:639	arg1	effect					619:624	the effect	615:624	the effect of temperature on population growth	615:660	In the present study, we observed the effect of temperature on population growth over five months using soil microcosms.					
33863429	7	61	theme	biomass	1121:1127	arg1	production					1129:1138	almost similar biomass production	1106:1138	almost similar biomass production	1106:1138	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	2	62	theme	protein	528:534	arg1	contents					516:523	high contents	511:523	high contents of protein and long-chain poly-unsaturated fatty acids	511:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	1	63	theme	larval	125:130	arg1	stages					132:137	the larval stages	121:137	the larval stages of many fish species grown in aquaculture	121:179	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	8	64	from	%	1294:1294	arg1	range					1234:1238	this temperature range	1217:1238	this temperature range	1217:1238	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	5	65	theme	rolled	824:829	arg1	libitum					839:845	rolled oats ad libitum	824:845	rolled oats ad libitum	824:845	Hatched enchytraeids were given rolled oats ad libitum as feed.					
33863429	2	66	theme	materials	493:501	arg1	range					470:474	a wide range	463:474	a wide range of organic waste materials	463:501	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	8	67	theme	dry	1336:1338	arg1	weight					1340:1345	dry weight	1336:1345	dry weight	1336:1345	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	2	68	contain	has	507:509	arg1	worm					335:338	white worm	329:338	white worm	329:338	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	68	contain	has	507:509	arg1	albidus					354:360	Enchytraeus albidus	342:360	Enchytraeus albidus	342:360	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	68	contain	has	507:509	arg1	enchytraeid					316:326	The terrestrial enchytraeid	300:326	The terrestrial enchytraeid (white worm)	300:339	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	68	contain	has	507:509	arg2	contents					516:523	high contents	511:523	high contents of protein and long-chain poly-unsaturated fatty acids	511:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	3	69	theme	soil	685:688	arg1	microcosms					690:699	soil microcosms	685:699	soil microcosms	685:699	In the present study, we observed the effect of temperature on population growth over five months using soil microcosms.					
33863429	2	70	contain	has	363:365	arg2	source					394:399	a sustainable source	380:399	a sustainable source of live feed	380:412	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	70	contain	has	363:365	arg2	potential					367:375	potential	367:375	potential	367:375	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	70	contain	has	363:365	arg1	albidus					354:360	Enchytraeus albidus	342:360	Enchytraeus albidus	342:360	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	70	contain	has	363:365	arg1	enchytraeid					316:326	The terrestrial enchytraeid	300:326	The terrestrial enchytraeid (white worm)	300:339	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	70	contain	has	363:365	arg1	worm					335:338	white worm	329:338	white worm	329:338	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	5	71	theme	ad	836:837	arg1	libitum					839:845	rolled oats ad libitum	824:845	rolled oats ad libitum	824:845	Hatched enchytraeids were given rolled oats ad libitum as feed.					
33863429	6	72	theme	4-25 °C	928:934	arg1	range					919:923	the range	915:923	the range of 4-25 °C	915:934	We followed the population growth at seven temperatures in the range of 4-25 °C and investigated body composition in order to find optimal temperature for mass production.					
33863429	2	73	theme	organic	479:485	arg1	materials					493:501	organic waste materials	479:501	organic waste materials	479:501	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	1	74	dep	feeds	215:219	arg1	exhibit					227:233	exhibit	227:233	feeds often exhibit higher survival and growth than those reared on formulated feed	215:297	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	0	75	theme	organism	53:60	arg1	albidus					74:80	the live feed organism Enchytraeus albidus	39:80	the live feed organism Enchytraeus albidus	39:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	5	76	theme	Hatched	792:798	arg1	enchytraeids					800:811	Hatched enchytraeids	792:811	Hatched enchytraeids	792:811	Hatched enchytraeids were given rolled oats ad libitum as feed.					
33863429	5	76	theme	Hatched	792:798	arg1	feed					850:853	feed	850:853	feed	850:853	Hatched enchytraeids were given rolled oats ad libitum as feed.					
33863429	0	77	theme	albidus	74:80	arg1	production					25:34	mass production	20:34	mass production of the live feed organism Enchytraeus albidus	20:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	12	78	theme	L-1	1763:1765	arg1	temperatures					1778:1789	80 g live worms L-1 month-1 at temperatures	1747:1789	80 g live worms L-1 month-1 at temperatures between 15 and 22 °C	1747:1810	Maximum production of biomass can likely reach 80 g live worms L-1 month-1 at temperatures between 15 and 22 °C.					
33863429	9	79	theme	fatty	1399:1403	arg1	composition					1410:1420	fatty acid composition	1399:1420	fatty acid composition	1399:1420	The temperature had a highly significant effect on fatty acid composition.					
33863429	2	80	theme	acids	574:578	arg1	contents					516:523	high contents	511:523	high contents of protein and long-chain poly-unsaturated fatty acids	511:578	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	8	81	theme	temperature	1222:1232	arg1	range					1234:1238	this temperature range	1217:1238	this temperature range	1217:1238	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	2	82	theme	Enchytraeus	342:352	arg1	albidus					354:360	Enchytraeus albidus	342:360	Enchytraeus albidus	342:360	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	82	theme	Enchytraeus	342:352	arg1	enchytraeid					316:326	The terrestrial enchytraeid	300:326	The terrestrial enchytraeid (white worm)	300:339	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	7	83	theme	thermal	1071:1077	arg1	range					1087:1091	a broad thermal optimum range	1063:1091	a broad thermal optimum range	1063:1091	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	12	84	theme	live	1752:1755	arg1	temperatures					1778:1789	80 g live worms L-1 month-1 at temperatures	1747:1789	80 g live worms L-1 month-1 at temperatures between 15 and 22 °C	1747:1810	Maximum production of biomass can likely reach 80 g live worms L-1 month-1 at temperatures between 15 and 22 °C.					
33863429	6	85	from	temperatures	899:910	arg1	range					919:923	the range	915:923	the range of 4-25 °C	915:934	We followed the population growth at seven temperatures in the range of 4-25 °C and investigated body composition in order to find optimal temperature for mass production.					
33863429	0	86	theme	mass	20:23	arg1	production					25:34	mass production	20:34	mass production of the live feed organism Enchytraeus albidus	20:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	1	87	theme	feed	88:91	arg1	organisms					93:101	Live feed organisms	83:101	Live feed organisms	83:101	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	9	88	contain	had	1364:1366	arg2	effect					1389:1394	a highly significant effect	1368:1394	a highly significant effect on fatty acid composition	1368:1420	The temperature had a highly significant effect on fatty acid composition.					
33863429	9	88	contain	had	1364:1366	arg1	temperature					1352:1362	The temperature	1348:1362	The temperature	1348:1362	The temperature had a highly significant effect on fatty acid composition.					
33863429	0	89	theme	live	43:46	arg1	albidus					74:80	the live feed organism Enchytraeus albidus	39:80	the live feed organism Enchytraeus albidus	39:80	Thermal optimum for mass production of the live feed organism Enchytraeus albidus.					
33863429	7	90	theme	growth	1179:1184	arg1	rates					1186:1190	specific growth rates	1170:1190	specific growth rates between 6.5 and 6.8%	1170:1211	Results showed that E. albidus has a broad thermal optimum range and displays almost similar biomass production in the range of 15-22 °C with specific growth rates between 6.5 and 6.8%.					
33863429	2	91	theme	live	404:407	arg1	feed					409:412	live feed	404:412	live feed	404:412	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	11	92	theme	worms	1567:1571	arg1	density					1556:1562	achievable density	1545:1562	achievable density of worms in mass cultures	1545:1588	For what concerns achievable density of worms in mass cultures, our results surpassed previous results and showed that densities close to 100 g L-1 substrate are realistic.					
33863429	8	93	theme	contents	1280:1287	arg1	%					1294:1294	glycogen contents 20-25%	1271:1294	glycogen contents 20-25%	1271:1294	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	1	94	theme	formulated	283:292	arg1	feed					294:297	formulated feed	283:297	formulated feed	283:297	Live feed organisms are essential for the larval stages of many fish species grown in aquaculture, and juvenile fish reared on live feeds often exhibit higher survival and growth than those reared on formulated feed.					
33863429	12	95	theme	Maximum	1700:1706	arg1	production					1708:1717	Maximum production	1700:1717	Maximum production of biomass	1700:1728	Maximum production of biomass can likely reach 80 g live worms L-1 month-1 at temperatures between 15 and 22 °C.					
33863429	6	96	theme	mass	1011:1014	arg1	production					1016:1025	mass production	1011:1025	mass production	1011:1025	We followed the population growth at seven temperatures in the range of 4-25 °C and investigated body composition in order to find optimal temperature for mass production.					
33863429	8	97	theme	total	1300:1304	arg1	contents					1317:1324	total fatty acid contents	1300:1324	total fatty acid contents	1300:1324	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
33863429	2	98	theme	white	329:333	arg1	enchytraeid					316:326	The terrestrial enchytraeid	300:326	The terrestrial enchytraeid (white worm)	300:339	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	2	98	theme	white	329:333	arg1	worm					335:338	white worm	329:338	white worm	329:338	The terrestrial enchytraeid (white worm), Enchytraeus albidus, has potential as a sustainable source of live feed because it can easily be mass produced, feeds on a wide range of organic waste materials and has high contents of protein and long-chain poly-unsaturated fatty acids.					
33863429	8	99	from	%	1268:1268	arg1	range					1234:1238	this temperature range	1217:1238	this temperature range	1217:1238	In this temperature range, protein contents were 40-45%, glycogen contents 20-25% and total fatty acid contents 15-20% of dry weight.					
32870644	8	0	theme	outdoor	1288:1294	arg1	exposure					1300:1307	outdoor sun exposure	1288:1307	outdoor sun exposure	1288:1307	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	1	1	contain	have	116:119	arg1	Exposure					63:70	Exposure	63:70	Exposure to excessive ultraviolet (UV) radiation	63:110	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	1	1	contain	have	116:119	arg2	effects					133:139	detrimental effects	121:139	detrimental effects	121:139	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	1	2	theme	human	144:148	arg1	health					150:155	human health	144:155	human health	144:155	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	6	3	theme	color-changing	961:974	arg1	tattoo					976:981	a color-changing tattoo	959:981	a color-changing tattoo that loses color upon exposure to UV	959:1018	The sensors resemble a color-changing tattoo that loses color upon exposure to UV.					
32870644	7	4	theme	sensors	1162:1168	arg1	properties					1118:1127	the mechanical properties	1103:1127	the mechanical properties (e.g., modulus, hardness) of the sensors	1103:1168	The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors.					
32870644	9	5	theme	wearable	1398:1405	arg1	sensors					1407:1413	wearable sensors	1398:1413	wearable sensors	1398:1413	Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV.					
32870644	3	6	theme	robust	506:511	arg1	sensors					540:546	robust, flexible, and wearable UV sensors	506:546	robust, flexible, and wearable UV sensors	506:546	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	8	7	theme	naked	1362:1366	arg1	eye					1368:1370	the naked eye	1358:1370	the naked eye	1358:1370	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	3	8	theme	wearable	528:535	arg1	sensors					540:546	robust, flexible, and wearable UV sensors	506:546	robust, flexible, and wearable UV sensors	506:546	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	8	9	from	decrease	1328:1335	arg1	color					1340:1344	color	1340:1344	color	1340:1344	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	2	10	theme	rapid	325:329	arg1	manufacturing					346:358	rapid and affordable manufacturing	325:358	rapid and affordable manufacturing of these sensors	325:375	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	3	11	theme	UV	537:538	arg1	sensors					540:546	robust, flexible, and wearable UV sensors	506:546	robust, flexible, and wearable UV sensors	506:546	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	2	12	theme	sensors	369:375	arg1	manufacturing					346:358	rapid and affordable manufacturing	325:358	rapid and affordable manufacturing of these sensors	325:375	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	11	13	theme	applications	1914:1925	arg1	applications					1914:1925	other applications	1908:1925	other applications	1908:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	11	13	theme	applications	1914:1925	arg1	variety					1897:1903	a variety	1895:1903	a variety of other applications	1895:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	3	14	theme	ink	477:479	arg1	composition					481:491	printable ink composition	467:491	printable ink composition	467:491	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	5	15	theme	titanium	723:730	arg1	dioxide					732:738	photoactive titanium dioxide	711:738	photoactive titanium dioxide nanoparticles	711:752	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	11	16	theme	ink	1716:1718	arg1	biocompatibility					1674:1689	biocompatibility	1674:1689	biocompatibility	1674:1689	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	11	16	theme	ink	1716:1718	arg1	printability					1696:1707	printability	1696:1707	printability	1696:1707	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	11	16	theme	ink	1716:1718	arg1	tunability					1662:1671	tunability	1662:1671	tunability	1662:1671	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	9	17	theme	sterilizing	1490:1500	arg1	devices					1502:1508	UV-based workspace sterilizing devices	1471:1508	UV-based workspace sterilizing devices	1471:1508	Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV.					
32870644	5	18	theme	dyes	895:898	arg1	degradation					880:890	photocatalytic degradation	865:890	photocatalytic degradation of dyes	865:898	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	8	19	theme	sun	1296:1298	arg1	exposure					1300:1307	outdoor sun exposure	1288:1307	outdoor sun exposure	1288:1307	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	5	20	theme	dioxide	732:738	arg1	nanoparticles					740:752	photoactive titanium dioxide nanoparticles	711:752	photoactive titanium dioxide nanoparticles	711:752	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	1	21	theme	excessive	75:83	arg1	radiation					102:110	excessive ultraviolet (UV) radiation	75:110	excessive ultraviolet (UV) radiation	75:110	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	4	22	theme	standalone	636:645	arg1	constructs					647:656	standalone constructs	636:656	standalone constructs	636:656	To fabricate the sensors, a color-changing hydrogel ink was first developed from which standalone constructs were 3D printed.					
32870644	0	23	theme	Printed	3:9	arg1	Sensors					26:32	3D Printed Hydrogel-Based Sensors	0:32	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.	0:61	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.					
32870644	11	24	theme	printing	1773:1780	arg1	methods					1782:1788	advanced 3D printing methods	1761:1788	advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications	1761:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	7	25	theme	ink	1039:1041	arg1	composition					1043:1053	ink composition	1039:1053	ink composition	1039:1053	The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors.					
32870644	11	26	dep	sensors	1814:1820	arg1	addition					1799:1806	addition	1799:1806	addition	1799:1806	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	1	27	theme	ultraviolet	85:95	arg1	radiation					102:110	excessive ultraviolet (UV) radiation	75:110	excessive ultraviolet (UV) radiation	75:110	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	11	28	theme	sensing	1870:1876	arg1	technologies					1878:1889	other sensing technologies	1864:1889	other sensing technologies for a variety of other applications	1864:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	3	29	theme	novel	415:419	arg1	procedure					453:461	a novel three-dimensional (3D) printing procedure	413:461	a novel three-dimensional (3D) printing procedure	413:461	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	0	30	theme	3D	0:1	arg1	Sensors					26:32	3D Printed Hydrogel-Based Sensors	0:32	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.	0:61	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.					
32870644	7	31	dep	properties	1118:1127	arg1	hardness					1145:1152	hardness	1145:1152	hardness	1145:1152	The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors.					
32870644	7	31	dep	properties	1118:1127	arg1	modulus					1136:1142	modulus	1136:1142	modulus	1136:1142	The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors.					
32870644	2	32	theme	large	382:386	arg1	scale					388:392	a large scale	380:392	a large scale	380:392	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	4	33	theme	color-changing	577:590	arg1	ink					601:603	a color-changing hydrogel ink	575:603	a color-changing hydrogel ink	575:603	To fabricate the sensors, a color-changing hydrogel ink was first developed from which standalone constructs were 3D printed.					
32870644	5	34	theme	blue	790:793	arg1	methylene					780:788	methylene blue	780:793	methylene blue	780:793	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	34	theme	blue	790:793	arg1	dyes					759:762	dyes	759:762	dyes (methyl orange, methylene blue, and malachite green)	759:815	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	1	35	theme	UV	98:99	arg1	radiation					102:110	excessive ultraviolet (UV) radiation	75:110	excessive ultraviolet (UV) radiation	75:110	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	11	36	theme	UV	1811:1812	arg1	sensors					1814:1820	UV sensors	1811:1820	UV sensors	1811:1820	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	5	37	theme	malachite	800:808	arg1	green					810:814	malachite green	800:814	malachite green	800:814	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	37	theme	malachite	800:808	arg1	dyes					759:762	dyes	759:762	dyes (methyl orange, methylene blue, and malachite green)	759:815	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	0	38	theme	Hydrogel-Based	11:24	arg1	Sensors					26:32	3D Printed Hydrogel-Based Sensors	0:32	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.	0:61	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.					
32870644	5	39	dep	dyes	759:762	arg1	orange					772:777	methyl orange	765:777	methyl orange	765:777	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	39	dep	dyes	759:762	arg1	green					810:814	malachite green	800:814	malachite green	800:814	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	39	dep	dyes	759:762	arg1	methylene					780:788	methylene blue	780:793	methylene blue	780:793	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	39	dep	dyes	759:762	arg1	dyes					759:762	dyes	759:762	dyes (methyl orange, methylene blue, and malachite green)	759:815	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	8	40	theme	visible	1347:1353	arg1	color					1340:1344	color	1340:1344	color	1340:1344	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	5	41	theme	photocatalytic	865:878	arg1	degradation					880:890	photocatalytic degradation	865:890	photocatalytic degradation of dyes	865:898	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	8	42	theme	one-step	1207:1214	arg1	fabrication					1216:1226	the one-step fabrication	1203:1226	the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye	1203:1370	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	7	43	theme	mechanical	1107:1116	arg1	properties					1118:1127	the mechanical properties	1103:1127	the mechanical properties (e.g., modulus, hardness) of the sensors	1103:1168	The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors.					
32870644	5	44	contain	contains	683:690	arg2	alginate					692:699	alginate	692:699	alginate	692:699	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg2	gelatin					702:708	gelatin	702:708	gelatin	702:708	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg1	ink					679:681	The ink	675:681	The ink	675:681	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg2	nanoparticles					740:752	photoactive titanium dioxide nanoparticles	711:752	photoactive titanium dioxide nanoparticles	711:752	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg2	green					810:814	malachite green	800:814	malachite green	800:814	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg2	orange					772:777	methyl orange	765:777	methyl orange	765:777	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg2	dyes					759:762	dyes	759:762	dyes (methyl orange, methylene blue, and malachite green)	759:815	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	44	contain	contains	683:690	arg2	methylene					780:788	methylene blue	780:793	methylene blue	780:793	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	2	45	theme	easy-to-use	170:180	arg1	sensors					182:188	Inexpensive easy-to-use sensors	158:188	Inexpensive easy-to-use sensors for monitoring UV radiation	158:216	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	11	46	theme	other	1864:1868	arg1	technologies					1878:1889	other sensing technologies	1864:1889	other sensing technologies for a variety of other applications	1864:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	3	47	theme	three-dimensional	421:437	arg1	procedure					453:461	a novel three-dimensional (3D) printing procedure	413:461	a novel three-dimensional (3D) printing procedure	413:461	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	5	48	theme	methyl	765:770	arg1	orange					772:777	methyl orange	765:777	methyl orange	765:777	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	5	48	theme	methyl	765:770	arg1	dyes					759:762	dyes	759:762	dyes (methyl orange, methylene blue, and malachite green)	759:815	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	9	49	contain	have	1430:1433	arg2	potential					1439:1447	the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV	1435:1568	the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV	1435:1568	Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV.					
32870644	9	49	contain	have	1430:1433	arg1	sensors					1422:1428	these sensors	1416:1428	these sensors	1416:1428	Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV.					
32870644	2	50	theme	Inexpensive	158:168	arg1	sensors					182:188	Inexpensive easy-to-use sensors	158:188	Inexpensive easy-to-use sensors for monitoring UV radiation	158:216	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	2	51	theme	broad-scale	228:238	arg1	assessment					240:249	broad-scale assessment	228:249	broad-scale assessment of UV exposure	228:264	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	3	52	theme	printing	444:451	arg1	procedure					453:461	a novel three-dimensional (3D) printing procedure	413:461	a novel three-dimensional (3D) printing procedure	413:461	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	11	53	theme	advanced	1761:1768	arg1	methods					1782:1788	advanced 3D printing methods	1761:1788	advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications	1761:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	11	54	theme	excellent	1726:1734	arg1	potential					1736:1744	excellent potential	1726:1744	excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications	1726:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	5	55	theme	dye	933:935	arg1	discoloration					912:924	discoloration	912:924	discoloration of the dye	912:935	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	8	56	theme	sensors	1251:1257	arg1	fabrication					1216:1226	the one-step fabrication	1203:1226	the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye	1203:1370	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	0	57	theme	UV	50:51	arg1	Exposure					53:60	UV Exposure	50:60	UV Exposure	50:60	3D Printed Hydrogel-Based Sensors for Quantifying UV Exposure.					
32870644	9	58	theme	UV-based	1471:1478	arg1	devices					1502:1508	UV-based workspace sterilizing devices	1471:1508	UV-based workspace sterilizing devices	1471:1508	Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV.					
32870644	5	59	used	used	848:851	arg2	nanoparticles					830:842	the nanoparticles	826:842	the nanoparticles	826:842	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
32870644	8	60	theme	stable	1244:1249	arg1	sensors					1251:1257	mechanically stable sensors	1231:1257	mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye	1231:1370	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	7	61	dep	modulus	1136:1142	arg1	e.g.					1130:1133	e.g.	1130:1133	e.g.	1130:1133	The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors.					
32870644	2	62	theme	UV	205:206	arg1	radiation					208:216	UV radiation	205:216	UV radiation	205:216	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	3	63	dep	three-dimensional	421:437	arg1	3D					440:441	3D	440:441	3D	440:441	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	2	64	theme	affordable	335:344	arg1	manufacturing					346:358	rapid and affordable manufacturing	325:358	rapid and affordable manufacturing of these sensors	325:375	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	4	65	theme	hydrogel	592:599	arg1	ink					601:603	a color-changing hydrogel ink	575:603	a color-changing hydrogel ink	575:603	To fabricate the sensors, a color-changing hydrogel ink was first developed from which standalone constructs were 3D printed.					
32870644	11	66	theme	other	1908:1912	arg1	applications					1914:1925	other applications	1908:1925	other applications	1908:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	2	67	theme	exposure	257:264	arg1	assessment					240:249	broad-scale assessment	228:249	broad-scale assessment of UV exposure	228:264	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	1	68	theme	detrimental	121:131	arg1	effects					133:139	detrimental effects	121:139	detrimental effects	121:139	Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health.					
32870644	3	69	theme	printable	467:475	arg1	composition					481:491	printable ink composition	467:491	printable ink composition	467:491	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	2	70	theme	UV	254:255	arg1	exposure					257:264	UV exposure	254:264	UV exposure	254:264	Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale.					
32870644	8	71	theme	optimized	1175:1183	arg1	procedure					1185:1193	The optimized procedure	1171:1193	The optimized procedure	1171:1193	The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye.					
32870644	9	72	theme	workspace	1480:1488	arg1	devices					1502:1508	UV-based workspace sterilizing devices	1471:1508	UV-based workspace sterilizing devices	1471:1508	Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV.					
32870644	10	73	dep	inexpensive	1587:1597	arg1	inexpensive					1587:1597	inexpensive	1587:1597	inexpensive	1587:1597	The sensors are inexpensive, stable, extremely robust, biodegradable, and easy to use.					
32870644	10	73	dep	inexpensive	1587:1597	arg1	sensors					1575:1581	The sensors	1571:1581	The sensors	1571:1581	The sensors are inexpensive, stable, extremely robust, biodegradable, and easy to use.					
32870644	10	73	dep	inexpensive	1587:1597	arg1	robust					1618:1623	robust	1618:1623	robust	1618:1623	The sensors are inexpensive, stable, extremely robust, biodegradable, and easy to use.					
32870644	3	74	theme	flexible	514:521	arg1	sensors					540:546	robust, flexible, and wearable UV sensors	506:546	robust, flexible, and wearable UV sensors	506:546	Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors.					
32870644	11	75	theme	3D	1770:1771	arg1	methods					1782:1788	advanced 3D printing methods	1761:1788	advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications	1761:1925	The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.					
32870644	5	76	theme	photoactive	711:721	arg1	dioxide					732:738	photoactive titanium dioxide	711:738	photoactive titanium dioxide nanoparticles	711:752	The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye.					
33808946	0	0	theme	PCL	65:67	arg1	Scaffold					89:96	a PCL Electrospun Fibrous Scaffold	63:96	a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications	63:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	4	1	dep	polycaprolactone	825:840	arg1	polymer					848:854	polymer	848:854	polymer	848:854	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	4	1	dep	polycaprolactone	825:840	arg1	HA					860:861	HA	860:861	HA	860:861	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	4	2	theme	core/shell	791:800	arg1	morphology					802:811	a core/shell morphology	789:811	a core/shell morphology composed of polycaprolactone (PCL) polymer and HA	789:861	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	8	3	theme	molecular	1441:1449	arg1	self-assembly					1451:1463	molecular self-assembly	1441:1463	molecular self-assembly	1441:1463	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	1	4	theme	Bone	149:152	arg1	engineering					161:171	Bone tissue engineering	149:171	Bone tissue engineering	149:171	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	1	4	theme	Bone	149:152	arg1	technique					217:225	a rapidly developing, minimally invasive technique	176:225	a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM)	176:362	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	1	5	theme	matrix	351:356	arg1	function					321:328	function	321:328	function	321:328	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	1	5	theme	matrix	351:356	arg1	structure					307:315	structure	307:315	structure	307:315	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	7	6	theme	biocompatibility	1167:1182	arg1	assays					1184:1189	In vitro biocompatibility assays	1158:1189	In vitro biocompatibility assays	1158:1189	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	2	7	contain	have	412:415	arg1	scaffolds					375:383	scaffolds	375:383	scaffolds made of electrospun fibers	375:410	Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity.					
33808946	2	7	contain	have	412:415	arg2	interest					425:432	aroused interest	417:432	aroused interest	417:432	Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity.					
33808946	1	8	theme	tissue	154:159	arg1	engineering					161:171	Bone tissue engineering	149:171	Bone tissue engineering	149:171	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	1	8	theme	tissue	154:159	arg1	technique					217:225	a rapidly developing, minimally invasive technique	176:225	a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM)	176:362	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	0	9	theme	Fibrous	81:87	arg1	Scaffold					89:96	a PCL Electrospun Fibrous Scaffold	63:96	a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications	63:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	1	10	theme	extracellular	337:349	arg1	ECM					359:361	ECM	359:361	ECM	359:361	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	1	10	theme	extracellular	337:349	arg1	matrix					351:356	the extracellular matrix	333:356	the extracellular matrix (ECM)	333:362	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	5	11	theme	acid	965:968	arg1	motif					976:980	the arginine-glycine-aspartic acid (RGD) motif	935:980	the arginine-glycine-aspartic acid (RGD) motif	935:980	The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition.					
33808946	6	12	theme	ECM	1143:1145	arg1	structure					1147:1155	the ECM structure	1139:1155	the ECM structure	1139:1155	Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure.					
33808946	8	13	theme	multi-component	1390:1404	arg1	approach					1406:1413	this multi-component approach	1385:1413	this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering	1385:1594	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	0	14	theme	Electrospun	69:79	arg1	Scaffold					89:96	a PCL Electrospun Fibrous Scaffold	63:96	a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications	63:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	1	15	dep	structure	307:315	arg1	the					303:305	the	303:305	the	303:305	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	7	16	theme	MC3T3-E1	1205:1212	arg1	preosteoblasts					1214:1227	MC3T3-E1 preosteoblasts	1205:1227	MC3T3-E1 preosteoblasts	1205:1227	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	6	17	theme	imaging	1067:1073	arg1	microscopy					1056:1065	Electron microscopy imaging	1047:1073	Electron microscopy imaging	1047:1073	Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure.					
33808946	4	18	theme	short	883:887	arg1	peptide					905:911	a short self-assembling peptide	881:911	a short self-assembling peptide	881:911	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	8	19	theme	leading	1527:1533	arg1	candidate					1535:1543	a leading candidate	1525:1543	a leading candidate to serve as a scaffold for bone tissue engineering	1525:1594	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	4	20	theme	composite	765:773	arg1	scaffold					775:782	a composite scaffold	763:782	a composite scaffold	763:782	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	7	21	theme	significant	1281:1291	arg1	differentiation					1304:1318	significant osteogenic differentiation	1281:1318	significant osteogenic differentiation	1281:1318	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	5	22	theme	molecular	1024:1032	arg1	recognition					1034:1044	molecular recognition	1024:1044	molecular recognition	1024:1044	The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition.					
33808946	0	23	theme	Scaffold	89:96	arg1	Performance					48:58	the Performance	44:58	the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications	44:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	6	24	theme	scaffold	1120:1127	arg1	network					1105:1111	the fibrous network	1093:1111	the fibrous network of the scaffold	1093:1127	Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure.					
33808946	4	25	used	used	729:732	arg2	we					726:727	we	726:727	we	726:727	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	7	26	theme	calcium	1324:1330	arg1	mineralization					1332:1345	calcium mineralization	1324:1345	calcium mineralization	1324:1345	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	3	27	theme	abundant	516:523	arg1	acid					500:503	Hyaluronic acid	489:503	Hyaluronic acid (HA)	489:508	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	27	theme	abundant	516:523	arg1	component					525:533	an abundant component	513:533	an abundant component of the ECM	513:544	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	7	28	theme	osteogenic	1293:1302	arg1	differentiation					1304:1318	significant osteogenic differentiation	1281:1318	significant osteogenic differentiation	1281:1318	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	0	29	theme	Hyaluronic	0:9	arg1	Acid					11:14	Hyaluronic Acid	0:14	Hyaluronic Acid	0:14	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	5	30	theme	cellular	995:1002	arg1	attachment					1004:1013	cellular attachment	995:1013	cellular attachment based on molecular recognition	995:1044	The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition.					
33808946	2	31	theme	aroused	417:423	arg1	interest					425:432	aroused interest	417:432	aroused interest	417:432	Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity.					
33808946	1	32	theme	biomaterial	270:280	arg1	scaffolds					282:290	biomaterial scaffolds	270:290	biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM)	270:362	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	0	33	theme	Bone	111:114	arg1	Applications					135:146	Bone Tissue Engineering Applications	111:146	Bone Tissue Engineering Applications	111:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	8	34	theme	bone	1572:1575	arg1	engineering					1584:1594	bone tissue engineering	1572:1594	bone tissue engineering	1572:1594	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	3	35	with	combination	686:696	arg1	polymer					711:717	another polymer	703:717	another polymer	703:717	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	1	36	theme	developing	186:195	arg1	technique					217:225	a rapidly developing, minimally invasive technique	176:225	a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM)	176:362	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	1	36	theme	developing	186:195	arg1	engineering					161:171	Bone tissue engineering	149:171	Bone tissue engineering	149:171	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	3	37	theme	regenerative	584:595	arg1	medicine					597:604	regenerative medicine	584:604	regenerative medicine	584:604	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	6	38	theme	fibrous	1097:1103	arg1	network					1105:1111	the fibrous network	1093:1111	the fibrous network of the scaffold	1093:1127	Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure.					
33808946	2	39	theme	electrospun	393:403	arg1	fibers					405:410	electrospun fibers	393:410	electrospun fibers	393:410	Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity.					
33808946	7	40	theme	In	1158:1159	arg1	assays					1184:1189	In vitro biocompatibility assays	1158:1189	In vitro biocompatibility assays	1158:1189	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	0	41	theme	Engineering	123:133	arg1	Applications					135:146	Bone Tissue Engineering Applications	111:146	Bone Tissue Engineering Applications	111:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	3	42	theme	ECM	542:544	arg1	acid					500:503	Hyaluronic acid	489:503	Hyaluronic acid (HA)	489:508	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	42	theme	ECM	542:544	arg1	material					564:571	an attractive material	550:571	an attractive material for use in regenerative medicine	550:604	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	42	theme	ECM	542:544	arg1	component					525:533	an abundant component	513:533	an abundant component of the ECM	513:544	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	0	43	theme	Short	22:26	arg1	Peptide					28:34	a Short Peptide	20:34	a Short Peptide	20:34	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	8	44	theme	tissue	1577:1582	arg1	engineering					1584:1594	bone tissue engineering	1572:1594	bone tissue engineering	1572:1594	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	0	45	theme	Tissue	116:121	arg1	Applications					135:146	Bone Tissue Engineering Applications	111:146	Bone Tissue Engineering Applications	111:146	Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.					
33808946	8	46	theme	molecular	1470:1478	arg1	motifs					1492:1497	molecular recognition motifs	1470:1497	molecular recognition motifs	1470:1497	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	2	47	theme	high	474:477	arg1	porosity					479:486	high porosity	474:486	high porosity	474:486	Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity.					
33808946	3	48	from	use	577:579	arg1	medicine					597:604	regenerative medicine	584:604	regenerative medicine	584:604	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	49	used	used	678:681	arg2	it					667:668	it	667:668	it	667:668	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	5	50	theme	RGD	971:973	arg1	motif					976:980	the arginine-glycine-aspartic acid (RGD) motif	935:980	the arginine-glycine-aspartic acid (RGD) motif	935:980	The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition.					
33808946	1	51	dep	the	259:261	arg1	aid					263:265	aid	263:265	aid	263:265	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	8	52	theme	approach	1406:1413	arg1	potential					1372:1380	the potential	1368:1380	the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering	1368:1594	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	1	53	with	bone	249:252	arg1	the					259:261	the	259:261	the	259:261	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	5	54	theme	arginine-glycine-aspartic	939:963	arg1	motif					976:980	the arginine-glycine-aspartic acid (RGD) motif	935:980	the arginine-glycine-aspartic acid (RGD) motif	935:980	The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition.					
33808946	7	55	dep	In	1158:1159	arg1	vitro					1161:1165	vitro	1161:1165	vitro	1161:1165	In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization.					
33808946	8	56	theme	recognition	1480:1490	arg1	motifs					1492:1497	molecular recognition motifs	1470:1497	molecular recognition motifs	1470:1497	Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering.					
33808946	6	57	theme	Electron	1047:1054	arg1	microscopy					1056:1065	Electron microscopy imaging	1047:1073	Electron microscopy imaging	1047:1073	Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure.					
33808946	4	58	dep	used	729:732	arg1	incorporating					867:879	incorporating	867:879	incorporating a short self-assembling peptide	867:911	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	4	58	dep	used	729:732	arg1	fabricate					753:761	fabricate	753:761	to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA	750:861	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	1	59	dep	developing	186:195	arg1	invasive					208:215	invasive	208:215	invasive	208:215	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
33808946	4	60	theme	self-assembling	889:903	arg1	peptide					905:911	a short self-assembling peptide	881:911	a short self-assembling peptide	881:911	Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide.					
33808946	3	61	theme	attractive	553:562	arg1	material					564:571	an attractive material	550:571	an attractive material for use in regenerative medicine	550:604	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	62	theme	Hyaluronic	489:498	arg1	HA					506:507	HA	506:507	HA	506:507	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	62	theme	Hyaluronic	489:498	arg1	acid					500:503	Hyaluronic acid	489:503	Hyaluronic acid (HA)	489:508	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	3	62	theme	Hyaluronic	489:498	arg1	component					525:533	an abundant component	513:533	an abundant component of the ECM	513:544	Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer.					
33808946	1	63	theme	lost	244:247	arg1	bone					249:252	lost bone	244:252	lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM)	244:362	Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM).					
34906850	2	0	theme	tumor	532:536	arg1	cells					538:542	cultured tumor cells	523:542	cultured tumor cells	523:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	5	1	theme	glycoblotting	1269:1281	arg1	tools					1310:1314	promising tools	1300:1314	promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site	1300:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	5	1	theme	glycoblotting	1269:1281	arg1	method					1283:1288	nanosomal glycoblotting method	1259:1288	nanosomal glycoblotting method	1259:1288	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	4	2	theme	key	971:973	arg1	glycotypes					975:984	key glycotypes	971:984	key glycotypes	971:984	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	1	3	theme	exosomes	170:177	arg1	importance					136:145	emerging importance	127:145	emerging importance of tumor cells-derived exosomes in cancer metastasis	127:198	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	2	4	theme	cultured	523:530	arg1	cells					538:542	cultured tumor cells	523:542	cultured tumor cells	523:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	4	5	theme	N-glycosylation	925:939	arg1	patterns					941:948	cancer cell-type-specific N-glycosylation patterns	899:948	cancer cell-type-specific N-glycosylation patterns	899:948	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	5	6	theme	N-glycosylation	1214:1228	arg1	patterns					1230:1237	cancer cell-type-specific N-glycosylation patterns	1188:1237	cancer cell-type-specific N-glycosylation patterns	1188:1237	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	0	7	theme	cancer	100:105	arg1	metastasis					107:116	the organotropic cancer metastasis	83:116	the organotropic cancer metastasis	83:116	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	3	8	theme	other	728:732	arg1	compositions					734:745	other compositions	728:745	other compositions of exosomes	728:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	3	9	theme	antiadhesive	562:573	arg1	dots					583:586	antiadhesive quantum dots	562:586	antiadhesive quantum dots	562:586	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	5	10	theme	primary	1443:1449	arg1	site					1458:1461	the primary cancer site	1439:1461	the primary cancer site	1439:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	0	11	theme	organotropic	87:98	arg1	metastasis					107:116	the organotropic cancer metastasis	83:116	the organotropic cancer metastasis	83:116	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	5	12	theme	vesicles	1412:1419	arg1	glycocalyx					1384:1393	the glycocalyx	1380:1393	the glycocalyx of extracellular vesicles disseminated from the primary cancer site	1380:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	4	13	from	clearance	803:811	arg1	mice					875:878	mice	875:878	mice	875:878	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	3	14	theme	nice	599:602	arg1	tool					604:607	a nice tool	597:607	a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes	597:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	1	15	theme	molecular	298:306	arg1	composition					308:318	their molecular composition	292:318	their molecular composition	292:318	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	4	16	theme	N-glycans	1041:1049	arg1	patterns					941:948	cancer cell-type-specific N-glycosylation patterns	899:948	cancer cell-type-specific N-glycosylation patterns	899:948	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	16	theme	N-glycans	1041:1049	arg1	compositions					955:966	the compositions	951:966	the compositions of key glycotypes	951:984	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	16	theme	N-glycans	1041:1049	arg1	abundances					1009:1018	particularly dominant abundances	987:1018	particularly dominant abundances of high mannose-type N-glycans	987:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	16	theme	N-glycans	1041:1049	arg1	sialylation					1077:1087	the position-specific sialylation	1055:1087	the position-specific sialylation	1055:1087	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	1	17	theme	cancer	182:187	arg1	metastasis					189:198	cancer metastasis	182:198	cancer metastasis	182:198	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	4	18	theme	mannose-type	1028:1039	arg1	N-glycans					1041:1049	high mannose-type N-glycans	1023:1049	high mannose-type N-glycans	1023:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	3	19	theme	exosomes	750:757	arg1	compositions					734:745	other compositions	728:745	other compositions of exosomes	728:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	1	20	gly	heterogeneity	205:217	arg1	populations					230:240	exosome populations	222:240	exosome populations	222:240	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	1	21	from	importance	136:145	arg1	metastasis					189:198	cancer metastasis	182:198	cancer metastasis	182:198	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	2	22	theme	cancer	476:481	arg1	"					497:497	an exosome model "fluorescent cancer glyconanosomes"	446:497	an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	446:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	2	23	theme	glyconanosomes	483:496	arg1	"					497:497	an exosome model "fluorescent cancer glyconanosomes"	446:497	an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	446:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	4	24	theme	position-specific	1059:1075	arg1	sialylation					1077:1087	the position-specific sialylation	1055:1087	the position-specific sialylation	1055:1087	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	25	theme	cell-type-specific	906:923	arg1	patterns					941:948	cancer cell-type-specific N-glycosylation patterns	899:948	cancer cell-type-specific N-glycosylation patterns	899:948	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	26	theme	high	1023:1026	arg1	N-glycans					1041:1049	high mannose-type N-glycans	1023:1049	high mannose-type N-glycans	1023:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	2	27	theme	pre-metastatic	416:429	arg1	sites					431:435	pre-metastatic sites	416:435	pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	416:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	0	28	theme	Antiadhesive	0:11	arg1	nanosome					13:20	Antiadhesive nanosome	0:20	Antiadhesive nanosome	0:20	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	1	29	theme	composition	308:318	arg1	characterization					272:287	systemic characterization	263:287	systemic characterization of their molecular composition, biogenesis, and functions	263:345	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	4	30	theme	cancer	899:904	arg1	patterns					941:948	cancer cell-type-specific N-glycosylation patterns	899:948	cancer cell-type-specific N-glycosylation patterns	899:948	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	31	dep	In	760:761	arg1	vivo					763:766	vivo	763:766	vivo	763:766	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	5	32	theme	cancer	1188:1193	arg1	patterns					1230:1237	cancer cell-type-specific N-glycosylation patterns	1188:1237	cancer cell-type-specific N-glycosylation patterns	1188:1237	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	2	33	theme	fluorescent	464:474	arg1	"					497:497	an exosome model "fluorescent cancer glyconanosomes"	446:497	an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	446:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	2	34	theme	model	457:461	arg1	"					497:497	an exosome model "fluorescent cancer glyconanosomes"	446:497	an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	446:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	1	35	theme	biogenesis	321:330	arg1	characterization					272:287	systemic characterization	263:287	systemic characterization of their molecular composition, biogenesis, and functions	263:345	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	3	36	theme	cancer	690:695	arg1	types					702:706	four cancer cell types	685:706	four cancer cell types without bias due to other compositions of exosomes	685:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	2	37	theme	exosome	449:455	arg1	"					497:497	an exosome model "fluorescent cancer glyconanosomes"	446:497	an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	446:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	0	38	theme	glycocalyx	38:47	arg1	role					30:33	role	30:33	role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis	30:116	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	5	39	theme	pre-metastatic	1345:1358	arg1	sites					1360:1364	pre-metastatic sites	1345:1364	pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site	1345:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	3	40	theme	cell	697:700	arg1	types					702:706	four cancer cell types	685:706	four cancer cell types without bias due to other compositions of exosomes	685:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	3	41	theme	pivotal	630:636	arg1	functions					638:646	the pivotal functions	626:646	the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes	626:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	1	42	theme	exosome	222:228	arg1	populations					230:240	exosome populations	222:240	exosome populations	222:240	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	4	43	gly	N-glycosylation	925:939	arg1	glycotypes					975:984	key glycotypes	971:984	key glycotypes	971:984	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	43	gly	N-glycosylation	925:939	arg1	N-glycans					1041:1049	high mannose-type N-glycans	1023:1049	high mannose-type N-glycans	1023:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	44	from	circulation	790:800	arg1	mice					875:878	mice	875:878	mice	875:878	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	1	45	link	cells-derived	156:168	arg1	exosomes					170:177	tumor cells-derived exosomes	150:177	tumor cells-derived exosomes	150:177	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	5	46	theme	cell-type-specific	1195:1212	arg1	patterns					1230:1237	cancer cell-type-specific N-glycosylation patterns	1188:1237	cancer cell-type-specific N-glycosylation patterns	1188:1237	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	4	47	theme	glyconanosomes	857:870	arg1	clearance					803:811	clearance	803:811	clearance	803:811	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	47	theme	glyconanosomes	857:870	arg1	circulation					790:800	circulation	790:800	circulation	790:800	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	47	theme	glyconanosomes	857:870	arg1	biodistribution					831:845	organotropic biodistribution	818:845	organotropic biodistribution	818:845	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	1	48	theme	populations	230:240	arg1	heterogeneity					205:217	the heterogeneity	201:217	the heterogeneity of exosome populations	201:240	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	0	49	theme	tumor	52:56	arg1	exosomes					71:78	tumor cell-derived exosomes	52:78	tumor cell-derived exosomes	52:78	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	1	50	theme	functions	337:345	arg1	characterization					272:287	systemic characterization	263:287	systemic characterization of their molecular composition, biogenesis, and functions	263:345	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	2	51	theme	cells	538:542	arg1	N-glycans					510:518	N-glycans	510:518	N-glycans of cultured tumor cells	510:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	5	52	theme	cancer	1124:1129	arg1	glyconanosomes					1131:1144	cancer glyconanosomes	1124:1144	cancer glyconanosomes	1124:1144	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	2	53	theme	novel	374:378	arg1	method					380:385	a novel method	372:385	a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells	372:542	This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model "fluorescent cancer glyconanosomes" displaying N-glycans of cultured tumor cells.					
34906850	4	54	theme	dominant	1000:1007	arg1	abundances					1009:1018	particularly dominant abundances	987:1018	particularly dominant abundances of high mannose-type N-glycans	987:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	5	55	theme	nanosomal	1259:1267	arg1	tools					1310:1314	promising tools	1300:1314	promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site	1300:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	5	55	theme	nanosomal	1259:1267	arg1	method					1283:1288	nanosomal glycoblotting method	1259:1288	nanosomal glycoblotting method	1259:1288	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	5	56	theme	glyconanosomes	1131:1144	arg1	biodistribution					1105:1119	organ biodistribution	1099:1119	organ biodistribution of cancer glyconanosomes	1099:1144	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	1	57	theme	emerging	127:134	arg1	importance					136:145	emerging importance	127:145	emerging importance of tumor cells-derived exosomes in cancer metastasis	127:198	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	5	58	theme	cancer	1451:1456	arg1	site					1458:1461	the primary cancer site	1439:1461	the primary cancer site	1439:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	4	59	theme	glycotypes	975:984	arg1	patterns					941:948	cancer cell-type-specific N-glycosylation patterns	899:948	cancer cell-type-specific N-glycosylation patterns	899:948	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	59	theme	glycotypes	975:984	arg1	compositions					955:966	the compositions	951:966	the compositions of key glycotypes	951:984	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	59	theme	glycotypes	975:984	arg1	abundances					1009:1018	particularly dominant abundances	987:1018	particularly dominant abundances of high mannose-type N-glycans	987:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	59	theme	glycotypes	975:984	arg1	sialylation					1077:1087	the position-specific sialylation	1055:1087	the position-specific sialylation	1055:1087	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	3	60	theme	due	721:723	arg1	bias					716:719	bias	716:719	bias due to other compositions of exosomes	716:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	3	61	theme	glycocalyx	655:664	arg1	functions					638:646	the pivotal functions	626:646	the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes	626:757	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	0	62	link	cell-derived	58:69	arg1	exosomes					71:78	tumor cell-derived exosomes	52:78	tumor cell-derived exosomes	52:78	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	5	63	theme	promising	1300:1308	arg1	tools					1310:1314	promising tools	1300:1314	promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site	1300:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	5	63	theme	promising	1300:1308	arg1	method					1283:1288	nanosomal glycoblotting method	1259:1288	nanosomal glycoblotting method	1259:1288	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	4	64	gly	sialylation	1077:1087	arg1	glycotypes					975:984	key glycotypes	971:984	key glycotypes	971:984	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	64	gly	sialylation	1077:1087	arg1	N-glycans					1041:1049	high mannose-type N-glycans	1023:1049	high mannose-type N-glycans	1023:1049	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	4	65	theme	organotropic	818:829	arg1	biodistribution					831:845	organotropic biodistribution	818:845	organotropic biodistribution	818:845	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	3	66	theme	quantum	575:581	arg1	dots					583:586	antiadhesive quantum dots	562:586	antiadhesive quantum dots	562:586	Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes.					
34906850	4	67	theme	In	760:761	arg1	imaging					768:774	In vivo imaging	760:774	In vivo imaging	760:774	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	0	68	theme	exosomes	71:78	arg1	glycocalyx					38:47	glycocalyx	38:47	glycocalyx of tumor cell-derived exosomes	38:78	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	4	69	theme	cancer	850:855	arg1	glyconanosomes					857:870	cancer glyconanosomes	850:870	cancer glyconanosomes	850:870	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	1	70	theme	tumor	150:154	arg1	exosomes					170:177	tumor cells-derived exosomes	150:177	tumor cells-derived exosomes	150:177	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	5	71	theme	extracellular	1398:1410	arg1	vesicles					1412:1419	extracellular vesicles	1398:1419	extracellular vesicles disseminated from the primary cancer site	1398:1461	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	0	72	theme	cell-derived	58:69	arg1	exosomes					71:78	tumor cell-derived exosomes	52:78	tumor cell-derived exosomes	52:78	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	0	73	from	role	30:33	arg1	metastasis					107:116	the organotropic cancer metastasis	83:116	the organotropic cancer metastasis	83:116	Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis.					
34906850	5	74	theme	organ	1099:1103	arg1	biodistribution					1105:1119	organ biodistribution	1099:1119	organ biodistribution of cancer glyconanosomes	1099:1144	Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-type-specific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site.					
34906850	4	75	from	biodistribution	831:845	arg1	mice					875:878	mice	875:878	mice	875:878	In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation.					
34906850	1	76	theme	cells-derived	156:168	arg1	exosomes					170:177	tumor cells-derived exosomes	150:177	tumor cells-derived exosomes	150:177	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
34906850	1	77	theme	systemic	263:270	arg1	characterization					272:287	systemic characterization	263:287	systemic characterization of their molecular composition, biogenesis, and functions	263:345	Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions.					
32331693	0	0	theme	emulsion	89:96	arg1	systems					98:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	4	1	theme	immobilized	814:824	arg1	water					826:830	immobilized water	814:830	immobilized water	814:830	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	5	2	theme	mixed	1064:1068	arg1	dispersions/emulsions					1070:1090	egg yolk/κ-carrageenan mixed dispersions/emulsions	1041:1090	egg yolk/κ-carrageenan mixed dispersions/emulsions	1041:1090	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	4	3	theme	nuclear	648:654	arg1	resonance					665:673	nuclear magnetic resonance	648:673	low field nuclear magnetic resonance (LF-NMR)	638:682	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	3	theme	nuclear	648:654	arg1	LF-NMR					676:681	LF-NMR	676:681	LF-NMR	676:681	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	4	from	field	642:646	arg1	Results					625:631	Results	625:631	Results from low field nuclear magnetic resonance (LF-NMR)	625:682	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	1	5	theme	mixed	234:238	arg1	dispersions					240:250	egg yolk/κ-carrageenan mixed dispersions	211:250	egg yolk/κ-carrageenan mixed dispersions	211:250	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	4	6	theme	gels	921:924	arg1	capacity					900:907	the water holding capacity	882:907	the water holding capacity of emulsion gels	882:924	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	2	7	from	viscosity	404:412	arg1	size					355:358	the mean droplet size	338:358	the mean droplet size	338:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	1	8	theme	dispersions	240:250	arg1	behavior					180:187	flocculation behavior	167:187	flocculation behavior	167:187	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	1	8	theme	dispersions	240:250	arg1	properties					197:206	gel properties	193:206	gel properties	193:206	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	4	9	theme	water	886:890	arg1	capacity					900:907	the water holding capacity	882:907	the water holding capacity of emulsion gels	882:924	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	1	10	from	influence	142:150	arg1	behavior					180:187	flocculation behavior	167:187	flocculation behavior	167:187	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	1	10	from	influence	142:150	arg1	properties					197:206	gel properties	193:206	gel properties	193:206	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	0	11	theme	systems	98:104	arg1	behavior					13:20	Flocculation behavior	0:20	Flocculation behavior	0:20	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	0	11	theme	systems	98:104	arg1	properties					30:39	gel properties	26:39	gel properties	26:39	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	4	12	theme	water	826:830	arg1	content					803:809	the content	799:809	the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil	799:968	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	5	13	dep	suggested	986:994	arg1	tailored					1099:1106	tailored	1099:1106	suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations	986:1146	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	4	14	dep	field	642:646	arg1	resonance					665:673	nuclear magnetic resonance	648:673	low field nuclear magnetic resonance (LF-NMR)	638:682	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	14	dep	field	642:646	arg1	LF-NMR					676:681	LF-NMR	676:681	LF-NMR	676:681	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	1	15	theme	emulsions	255:263	arg1	behavior					180:187	flocculation behavior	167:187	flocculation behavior	167:187	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	1	15	theme	emulsions	255:263	arg1	properties					197:206	gel properties	193:206	gel properties	193:206	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	3	16	from	decrease	595:602	arg1	cohesiveness					611:622	gel cohesiveness	607:622	gel cohesiveness	607:622	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	3	16	from	decrease	595:602	arg1	strength					582:589	gel strength	578:589	gel strength	578:589	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	5	17	theme	egg	1041:1043	arg1	dispersions/emulsions					1070:1090	egg yolk/κ-carrageenan mixed dispersions/emulsions	1041:1090	egg yolk/κ-carrageenan mixed dispersions/emulsions	1041:1090	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	3	18	from	increase	566:573	arg1	cohesiveness					611:622	gel cohesiveness	607:622	gel cohesiveness	607:622	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	3	18	from	increase	566:573	arg1	strength					582:589	gel strength	578:589	gel strength	578:589	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	3	19	theme	NaCl	540:543	arg1	concentration					523:535	the concentration	519:535	the concentration of NaCl	519:543	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	4	20	theme	holding	892:898	arg1	capacity					900:907	the water holding capacity	882:907	the water holding capacity of emulsion gels	882:924	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	5	21	theme	yolk/κ-carrageenan	1045:1062	arg1	dispersions/emulsions					1070:1090	egg yolk/κ-carrageenan mixed dispersions/emulsions	1041:1090	egg yolk/κ-carrageenan mixed dispersions/emulsions	1041:1090	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	4	22	theme	hydration	751:759	arg1	ability					761:767	the hydration ability	747:767	the hydration ability of gel molecules	747:784	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	2	23	from	decrease	326:333	arg1	size					355:358	the mean droplet size	338:358	the mean droplet size	338:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	4	24	theme	NaCl	715:718	arg1	addition					703:710	the addition	699:710	the addition of NaCl	699:718	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	1	25	theme	NaCl	155:158	arg1	influence					142:150	the influence	138:150	the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions	138:263	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	4	26	theme	oil	966:968	arg1	incorporation					949:961	the incorporation	945:961	the incorporation of oil	945:968	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	27	dep	hydrogels	835:843	arg1	increased					865:873	increased	865:873	increased	865:873	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	0	28	theme	aqueous	77:83	arg1	systems					98:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	2	29	theme	NaCl	294:297	arg1	incorporation					299:311	NaCl incorporation	294:311	NaCl incorporation	294:311	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	30	theme	droplet	347:353	arg1	size					355:358	the mean droplet size	338:358	the mean droplet size	338:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	0	31	theme	Flocculation	0:11	arg1	behavior					13:20	Flocculation behavior	0:20	Flocculation behavior	0:20	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	5	32	theme	food	1130:1133	arg1	formulations					1135:1146	food formulations	1130:1146	food formulations	1130:1146	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	5	33	theme	flocculation	1000:1011	arg1	state					1013:1017	flocculation state	1000:1017	flocculation state	1000:1017	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	2	34	theme	mean	342:345	arg1	size					355:358	the mean droplet size	338:358	the mean droplet size	338:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	0	35	theme	gel	26:28	arg1	properties					30:39	gel properties	26:39	gel properties	26:39	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	3	36	theme	significant	554:564	arg1	increase					566:573	a significant increase	552:573	a significant increase in gel strength	552:589	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	4	37	theme	molecules	776:784	arg1	ability					761:767	the hydration ability	747:767	the hydration ability of gel molecules	747:784	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	1	38	theme	flocculation	167:178	arg1	behavior					180:187	flocculation behavior	167:187	flocculation behavior	167:187	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	0	39	theme	NaCl	117:120	arg1	Effect					107:112	Effect	107:112	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.	0:121	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	5	40	dep	state	1013:1017	arg1	the					996:998	the	996:998	the	996:998	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	4	41	theme	emulsion	912:919	arg1	gels					921:924	emulsion gels	912:924	emulsion gels	912:924	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	2	42	theme	dispersions/emulsions	427:447	arg1	decrease					326:333	a decrease	324:333	a decrease in the mean droplet size	324:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	42	theme	dispersions/emulsions	427:447	arg1	viscosity					404:412	viscosity	404:412	viscosity of the mixed dispersions/emulsions	404:447	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	42	theme	dispersions/emulsions	427:447	arg1	potential					366:374	zeta potential	361:374	zeta potential	361:374	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	42	theme	dispersions/emulsions	427:447	arg1	degree					377:382	degree	377:382	degree of flocculation	377:398	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	43	theme	mixed	421:425	arg1	dispersions/emulsions					427:447	the mixed dispersions/emulsions	417:447	the mixed dispersions/emulsions	417:447	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	5	44	theme	gel	1023:1025	arg1	properties					1027:1036	gel properties	1023:1036	gel properties	1023:1036	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	2	45	theme	flocculation	387:398	arg1	decrease					326:333	a decrease	324:333	a decrease in the mean droplet size	324:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	45	theme	flocculation	387:398	arg1	viscosity					404:412	viscosity	404:412	viscosity of the mixed dispersions/emulsions	404:447	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	45	theme	flocculation	387:398	arg1	potential					366:374	zeta potential	361:374	zeta potential	361:374	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	45	theme	flocculation	387:398	arg1	degree					377:382	degree	377:382	degree of flocculation	377:398	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	1	46	theme	gel	193:195	arg1	properties					197:206	gel properties	193:206	gel properties	193:206	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	0	47	theme	yolk/κ-carrageenan	48:65	arg1	systems					98:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	2	48	theme	gelation	473:480	arg1	point					464:468	the onset point	454:468	the onset point of gelation	454:480	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	0	49	theme	egg	44:46	arg1	systems					98:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	4	50	theme	hydrogels	835:843	arg1	content					803:809	the content	799:809	the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil	799:968	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	51	theme	gel	772:774	arg1	molecules					776:784	gel molecules	772:784	gel molecules	772:784	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	52	theme	magnetic	656:663	arg1	resonance					665:673	nuclear magnetic resonance	648:673	low field nuclear magnetic resonance (LF-NMR)	638:682	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	52	theme	magnetic	656:663	arg1	LF-NMR					676:681	LF-NMR	676:681	LF-NMR	676:681	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	4	53	theme	gel	852:854	arg1	strength					856:863	the gel strength	848:863	the gel strength	848:863	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	3	54	theme	gel	607:609	arg1	cohesiveness					611:622	gel cohesiveness	607:622	gel cohesiveness	607:622	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	3	55	theme	gel	578:580	arg1	strength					582:589	gel strength	578:589	gel strength	578:589	Increasing the concentration of NaCl led to a significant increase in gel strength and decrease in gel cohesiveness.					
32331693	2	56	from	potential	366:374	arg1	size					355:358	the mean droplet size	338:358	the mean droplet size	338:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	57	theme	incorporation	299:311	arg1	forward					499:505	forward	499:505	forward	499:505	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	57	theme	incorporation	299:311	arg1	result					284:289	a result	282:289	a result of NaCl incorporation	282:311	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	58	theme	onset	458:462	arg1	point					464:468	the onset point	454:468	the onset point of gelation	454:480	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	2	59	theme	zeta	361:364	arg1	potential					366:374	zeta potential	361:374	zeta potential	361:374	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	0	60	dep	behavior	13:20	arg1	Effect					107:112	Effect	107:112	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.	0:121	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	4	61	theme	low	638:640	arg1	field					642:646	low field nuclear magnetic resonance (LF-NMR)	638:682	low field nuclear magnetic resonance (LF-NMR)	638:682	Results from low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of NaCl could significantly reduce the hydration ability of gel molecules and increase the content of immobilized water of hydrogels as the gel strength increased, while the water holding capacity of emulsion gels was depressed with the incorporation of oil.					
32331693	1	62	theme	egg	211:213	arg1	dispersions					240:250	egg yolk/κ-carrageenan mixed dispersions	211:250	egg yolk/κ-carrageenan mixed dispersions	211:250	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	0	63	theme	composite	67:75	arg1	systems					98:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	egg yolk/κ-carrageenan composite aqueous and emulsion systems	44:104	Flocculation behavior and gel properties of egg yolk/κ-carrageenan composite aqueous and emulsion systems: Effect of NaCl.					
32331693	5	64	theme	dispersions/emulsions	1070:1090	arg1	state					1013:1017	flocculation state	1000:1017	flocculation state	1000:1017	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	5	64	theme	dispersions/emulsions	1070:1090	arg1	properties					1027:1036	gel properties	1023:1036	gel properties	1023:1036	These findings suggested the flocculation state and gel properties of egg yolk/κ-carrageenan mixed dispersions/emulsions can be tailored by adjusting NaCl for food formulations.					
32331693	1	65	dep	behavior	180:187	arg1	the					163:165	the	163:165	the	163:165	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32331693	2	66	from	degree	377:382	arg1	size					355:358	the mean droplet size	338:358	the mean droplet size	338:358	As a result of NaCl incorporation, there was a decrease in the mean droplet size, zeta potential, degree of flocculation and viscosity of the mixed dispersions/emulsions, and the onset point of gelation was also brought forward.					
32331693	1	67	theme	yolk/κ-carrageenan	215:232	arg1	dispersions					240:250	egg yolk/κ-carrageenan mixed dispersions	211:250	egg yolk/κ-carrageenan mixed dispersions	211:250	In this study, the influence of NaCl on the flocculation behavior and gel properties of egg yolk/κ-carrageenan mixed dispersions or emulsions were studied.					
32297553	8	0	theme	weight	1594:1599	arg1	gain					1601:1604	weight gain	1594:1604	weight gain	1594:1604	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	3	1	theme	HFHS	423:426	arg1	diet					428:431	a HFHS diet	421:431	a HFHS diet	421:431	Interestingly, sex differences in the response to a HFHS diet are emerging.					
32297553	0	2	theme	sucrose	48:54	arg1	diet					56:59	high sucrose diet	43:59	high sucrose diet	43:59	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	0	2	theme	sucrose	48:54	arg1	fat					38:40	a high fat	31:40	a high fat	31:40	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	0	3	from	differences	4:14	arg1	response					19:26	response	19:26	response to a high fat, high sucrose diet in both the gut	19:75	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	4	4	theme	Male	707:710	arg1	mice					723:726	Male and female mice	707:726	Male and female mice fed the HFHS diet	707:744	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	8	5	dep	gut	1467:1469	arg1	the					1463:1465	the	1463:1465	the	1463:1465	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	6	theme	LFLS-fed	784:791	arg1	mice					793:796	LFLS-fed mice	784:796	LFLS-fed mice	784:796	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	5	7	theme	previous	826:833	arg1	studies					835:841	previous studies	826:841	previous studies	826:841	However, in agreement with previous studies, males gained significantly more weight on the HFHS diet compared to females fed the same diet.					
32297553	8	8	theme	other	1261:1265	arg1	hand					1267:1270	the other hand	1257:1270	the other hand	1257:1270	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	6	9	theme	gut	1014:1016	arg1	composition					1029:1039	gut microbiome composition	1014:1039	gut microbiome composition	1014:1039	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32297553	4	10	from	diet	545:548	arg1	mice.Methods					588:599	8 week old male and female C57Bl/6 mice.Methods	553:599	8 week old male and female C57Bl/6 mice.Methods	553:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	1	11	theme	primary	210:216	arg1	concern					225:231	a primary health concern	208:231	a primary health concern	208:231	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.					
32297553	7	12	theme	less	1115:1118	arg1	densitometry					1130:1141	significantly less astrocyte densitometry	1101:1141	significantly less astrocyte densitometry	1101:1141	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	0	13	from	fat	38:40	arg1	gut					73:75	both the gut	64:75	both the gut	64:75	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	4	14	theme	female	716:721	arg1	mice					723:726	Male and female mice	707:726	Male and female mice fed the HFHS diet	707:744	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	8	15	theme	male	1631:1634	arg1	mice					1647:1650	male and female mice	1631:1650	male and female mice	1631:1650	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	2	16	theme	diet	279:282	arg1	Consumption					234:244	Consumption	234:244	Consumption of a high fat, high sugar (HFHS) diet	234:282	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	1	17	theme	health	218:223	arg1	concern					225:231	a primary health concern	208:231	a primary health concern	208:231	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.					
32297553	4	18	theme	male	564:567	arg1	mice.Methods					588:599	8 week old male and female C57Bl/6 mice.Methods	553:599	8 week old male and female C57Bl/6 mice.Methods	553:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	5	19	theme	same	928:931	arg1	diet					933:936	the same diet	924:936	the same diet	924:936	However, in agreement with previous studies, males gained significantly more weight on the HFHS diet compared to females fed the same diet.					
32297553	8	20	theme	female	1640:1645	arg1	mice					1647:1650	male and female mice	1631:1650	male and female mice	1631:1650	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	6	21	theme	diet-induced	986:997	arg1	differences					999:1009	significant sex and diet-induced differences	966:1009	differences	999:1009	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32297553	8	22	theme	hypothalamic	1305:1316	arg1	astrogliosis					1318:1329	hypothalamic astrogliosis	1305:1329	hypothalamic astrogliosis	1305:1329	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	2	23	theme	gut	309:311	arg1	dysbiosis					313:321	gut dysbiosis	309:321	gut dysbiosis	309:321	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	7	24	dep	observed	1092:1099	arg1	compared					1229:1236	compared	1229:1236	compared to Female LFLS	1229:1251	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	7	25	theme	astrocyte	1120:1128	arg1	densitometry					1130:1141	significantly less astrocyte densitometry	1101:1141	significantly less astrocyte densitometry	1101:1141	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	7	26	theme	microglial	1172:1181	arg1	morphology					1183:1192	microglial morphology	1172:1192	microglial morphology	1172:1192	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	0	27	theme	Sex	0:2	arg1	differences					4:14	Sex differences	0:14	Sex differences in response to a high fat, high sucrose diet in both the gut	0:75	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	2	28	theme	fat	256:258	arg1	diet					279:282	a high fat, high sugar (HFHS) diet	249:282	diet	279:282	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	7	29	theme	HFHS	1224:1227	arg1	hypothalamus					1201:1212	the hypothalamus	1197:1212	the hypothalamus of Female HFHS	1197:1227	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	4	30	theme	LFLS	539:542	arg1	diet					545:548	a low fat, low sugar (LFLS) diet	517:548	diet	545:548	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	5	31	theme	more	871:874	arg1	weight					876:881	significantly more weight	857:881	significantly more weight	857:881	However, in agreement with previous studies, males gained significantly more weight on the HFHS diet compared to females fed the same diet.					
32297553	2	32	theme	high	251:254	arg1	diet					279:282	a high fat, high sugar (HFHS) diet	249:282	diet	279:282	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	4	33	theme	fat	523:525	arg1	diet					545:548	a low fat, low sugar (LFLS) diet	517:548	diet	545:548	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	4	34	theme	C57Bl/6	580:586	arg1	mice.Methods					588:599	8 week old male and female C57Bl/6 mice.Methods	553:599	8 week old male and female C57Bl/6 mice.Methods	553:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	8	35	theme	Male	1371:1374	arg1	LFLS.Discussion					1376:1390	Male LFLS.Discussion	1371:1390	Male LFLS.Discussion	1371:1390	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	8	36	theme	gut	1576:1578	arg1	microbiome					1580:1589	the gut microbiome	1572:1589	the gut microbiome to weight gain and neuroinflammation	1572:1626	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	37	theme	more	767:770	arg1	weight					772:777	significantly more weight	753:777	significantly more weight than LFLS-fed mice	753:796	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	8	38	theme	direct	1557:1562	arg1	role					1564:1567	the direct role	1553:1567	the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice	1553:1650	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	39	theme	low	519:521	arg1	diet					545:548	a low fat, low sugar (LFLS) diet	517:548	diet	545:548	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	7	40	theme	significant	1150:1160	arg1	change					1162:1167	no significant change	1147:1167	no significant change to microglial morphology	1147:1192	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	4	41	theme	low	528:530	arg1	diet					545:548	a low fat, low sugar (LFLS) diet	517:548	diet	545:548	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	7	42	theme	Female	1241:1246	arg1	LFLS					1248:1251	Female LFLS	1241:1251	Female LFLS	1241:1251	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	8	43	from	role	1564:1567	arg1	mice					1647:1650	male and female mice	1631:1650	male and female mice	1631:1650	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	2	44	theme	increased	324:332	arg1	inflammation					334:345	increased inflammation	324:345	increased inflammation	324:345	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	5	45	with	agreement	811:819	arg1	studies					835:841	previous studies	826:841	previous studies	826:841	However, in agreement with previous studies, males gained significantly more weight on the HFHS diet compared to females fed the same diet.					
32297553	0	46	theme	hypothalamic	92:103	arg1	astrocytes					105:114	hypothalamic astrocytes	92:114	hypothalamic astrocytes	92:114	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	3	47	theme	sex	386:388	arg1	differences					390:400	sex differences	386:400	sex differences in the response to a HFHS diet	386:431	Interestingly, sex differences in the response to a HFHS diet are emerging.					
32297553	6	48	theme	Illumina	1063:1070	arg1	sequencing					1072:1081	next generation Illumina sequencing	1047:1081	next generation Illumina sequencing	1047:1081	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32297553	2	49	theme	HFHS	273:276	arg1	diet					279:282	a high fat, high sugar (HFHS) diet	249:282	diet	279:282	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	0	50	theme	high	33:36	arg1	diet					56:59	high sucrose diet	43:59	high sucrose diet	43:59	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	0	50	theme	high	33:36	arg1	fat					38:40	a high fat	31:40	a high fat	31:40	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	8	51	theme	diet-induced	1430:1441	arg1	differences					1443:1453	diet-induced differences	1430:1453	diet-induced differences in both the gut and the brain	1430:1483	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	52	theme	food	659:662	arg1	consumption					664:674	food consumption	659:674	food consumption	659:674	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	3	53	from	differences	390:400	arg1	response					409:416	the response	405:416	the response to a HFHS diet	405:431	Interestingly, sex differences in the response to a HFHS diet are emerging.					
32297553	4	54	theme	old	560:562	arg1	mice.Methods					588:599	8 week old male and female C57Bl/6 mice.Methods	553:599	8 week old male and female C57Bl/6 mice.Methods	553:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	6	55	theme	generation	1052:1061	arg1	sequencing					1072:1081	next generation Illumina sequencing	1047:1081	next generation Illumina sequencing	1047:1081	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32297553	8	56	theme	Male	1273:1276	arg1	HFHS					1278:1281	Male HFHS	1273:1281	Male HFHS	1273:1281	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	1	57	from	population	178:187	arg1	epidemic					162:169	epidemic	162:169	epidemic	162:169	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.					
32297553	1	57	from	population	178:187	arg1	Obesity					143:149	Obesity	143:149	Obesity	143:149	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.					
32297553	1	57	from	population	178:187	arg1	Objectives					131:140	Objectives	131:140	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.	131:232	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.					
32297553	7	58	theme	Female	1217:1222	arg1	HFHS					1224:1227	Female HFHS	1217:1227	Female HFHS	1217:1227	We also observed significantly less astrocyte densitometry and no significant change to microglial morphology in the hypothalamus of Female HFHS compared to Female LFLS.					
32297553	6	59	theme	next	1047:1050	arg1	sequencing					1072:1081	next generation Illumina sequencing	1047:1081	next generation Illumina sequencing	1047:1081	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32297553	1	60	from	epidemic	162:169	arg1	population					178:187	our population	174:187	our population	174:187	Objectives: Obesity is a major epidemic in our population and has emerged as a primary health concern.					
32297553	2	61	theme	sugar	266:270	arg1	diet					279:282	a high fat, high sugar (HFHS) diet	249:282	diet	279:282	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	6	62	theme	significant	966:976	arg1	sex					978:980	significant sex and diet-induced differences	966:1009	sex	978:980	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32297553	8	63	from	differences	1443:1453	arg1	gut					1467:1469	gut	1467:1469	gut	1467:1469	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	8	63	from	differences	1443:1453	arg1	brain					1479:1483	the brain	1475:1483	the brain	1475:1483	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	64	theme	HFHS	736:739	arg1	diet					741:744	the HFHS diet	732:744	the HFHS diet	732:744	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	4	65	theme	female	573:578	arg1	mice.Methods					588:599	8 week old male and female C57Bl/6 mice.Methods	553:599	8 week old male and female C57Bl/6 mice.Methods	553:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	2	66	theme	high	261:264	arg1	diet					279:282	a high fat, high sugar (HFHS) diet	249:282	diet	279:282	Consumption of a high fat, high sugar (HFHS) diet can specifically lead to gut dysbiosis, increased inflammation, and neuroinflammation.					
32297553	8	67	theme	future	1495:1500	arg1	studies					1502:1508	future studies	1495:1508	future studies	1495:1508	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	68	theme	diet	500:503	arg1	effects					482:488	the effects	478:488	the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods	478:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	0	69	theme	high	43:46	arg1	diet					56:59	high sucrose diet	43:59	high sucrose diet	43:59	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	0	69	theme	high	43:46	arg1	fat					38:40	a high fat	31:40	a high fat	31:40	Sex differences in response to a high fat, high sucrose diet in both the gut microbiome and hypothalamic astrocytes and microglia.					
32297553	5	70	theme	HFHS	890:893	arg1	diet					895:898	the HFHS diet	886:898	the HFHS diet compared to females fed the same diet	886:936	However, in agreement with previous studies, males gained significantly more weight on the HFHS diet compared to females fed the same diet.					
32297553	4	71	theme	body	642:645	arg1	weights					647:653	body weights	642:653	body weights	642:653	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	8	72	theme	microbiome	1580:1589	arg1	role					1564:1567	the direct role	1553:1567	the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice	1553:1650	On the other hand, Male HFHS revealed no change to hypothalamic astrogliosis, but increased microgliosis compared to Male LFLS.Discussion: In this study, we determined sex and diet-induced differences in both the gut and the brain, however, future studies will need to be performed in order to test the direct role of the gut microbiome to weight gain and neuroinflammation in male and female mice.					
32297553	4	73	theme	sugar	532:536	arg1	diet					545:548	a low fat, low sugar (LFLS) diet	517:548	diet	545:548	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	4	74	theme	HFHS	495:498	arg1	diet					500:503	a HFHS diet	493:503	a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods	493:599	In this study, we investigated the effects of a HFHS diet compared to a low fat, low sugar (LFLS) diet in 8 week old male and female C57Bl/6 mice.Methods: The diet was administered for 14 weeks; body weights and food consumption were evaluated weekly.Results: Male and female mice fed the HFHS diet gained significantly more weight than LFLS-fed mice.					
32297553	6	75	theme	microbiome	1018:1027	arg1	composition					1029:1039	gut microbiome composition	1014:1039	gut microbiome composition	1014:1039	Importantly, we determined significant sex and diet-induced differences to gut microbiome composition using next generation Illumina sequencing.					
32814101	0	0	theme	biological	101:110	arg1	activity					130:137	biological and antibacterial activity	101:137	activity	130:137	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	4	1	contain	have	731:734	arg2	cytotoxicity					754:765	no cytotoxicity	751:765	no cytotoxicity	751:765	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	4	1	contain	have	731:734	arg2	porosity					741:748	high porosity	736:748	high porosity	736:748	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	4	1	contain	have	731:734	arg2	Mg					820:821	Mg	820:821	Mg(OH)	820:825	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	4	1	contain	have	731:734	arg2	OH					823:824	OH	823:824	OH	823:824	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	4	1	contain	have	731:734	arg2	adaptation					787:796	excellent cellular adaptation	768:796	excellent cellular adaptation	768:796	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	4	1	contain	have	731:734	arg1	composite					708:716	The composite	704:716	The composite	704:716	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	7	2	theme	mentioned	1239:1247	arg1	nanobiocomposite					1249:1264	the mentioned nanobiocomposite	1235:1264	the mentioned nanobiocomposite	1235:1264	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	7	2	theme	mentioned	1239:1247	arg1	scaffold					1289:1296	a scaffold	1287:1296	a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems	1287:1377	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	6	3	theme	Hemolytic	997:1005	arg1	assay					1007:1011	Hemolytic assay	997:1011	Hemolytic assay	997:1011	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	1	4	theme	silk	282:285	arg1	fibroin					287:293	silk fibroin	282:293	silk fibroin	282:293	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	0	5	theme	antibacterial	116:128	arg1	activity					130:137	biological and antibacterial activity	101:137	activity	130:137	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	6	6	theme	metabolic	1022:1030	arg1	activity					1032:1039	cell metabolic activity	1017:1039	cell metabolic activity	1017:1039	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	4	7	theme	bacteria	891:898	arg1	growth					881:886	the growth	877:886	the growth of bacteria	877:898	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	5	8	theme	due	972:974	arg1	strength					963:970	the scaffold strength	950:970	the scaffold strength due to mechanical tests	950:994	In addition, silk fibroin and alginate increased the scaffold strength due to mechanical tests.					
32814101	1	9	theme	fibroin	287:293	arg1	nanorods					319:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	2	10	theme	X-Ray	557:561	arg1	analyses					569:576	energy dispersive X-Ray (EDX) analyses	539:576	energy dispersive X-Ray (EDX) analyses	539:576	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	0	11	with	scaffold	87:94	arg1	properties					163:172	improved mechanical properties	143:172	improved mechanical properties	143:172	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	0	11	with	scaffold	87:94	arg1	activity					130:137	biological and antibacterial activity	101:137	activity	130:137	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	7	12	from	use	1311:1313	arg1	dressing					1324:1331	wound dressing	1318:1331	wound dressing	1318:1331	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	7	12	from	use	1311:1313	arg1	engineering					1341:1351	tissue engineering	1334:1351	tissue engineering	1334:1351	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	7	12	from	use	1311:1313	arg1	systems					1371:1377	drug delivery systems	1357:1377	drug delivery systems	1357:1377	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	6	13	theme	cell	1017:1020	arg1	activity					1032:1039	cell metabolic activity	1017:1039	cell metabolic activity	1017:1039	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	2	14	theme	dispersive	546:555	arg1	EDX					564:566	EDX	564:566	EDX	564:566	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	14	theme	dispersive	546:555	arg1	X-Ray					557:561	energy dispersive X-Ray	539:561	energy dispersive X-Ray (EDX) analyses	539:576	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	5	15	theme	silk	914:917	arg1	fibroin					919:925	silk fibroin	914:925	silk fibroin	914:925	In addition, silk fibroin and alginate increased the scaffold strength due to mechanical tests.					
32814101	5	16	theme	mechanical	979:988	arg1	tests					990:994	mechanical tests	979:994	mechanical tests	979:994	In addition, silk fibroin and alginate increased the scaffold strength due to mechanical tests.					
32814101	7	17	theme	wound	1318:1322	arg1	dressing					1324:1331	wound dressing	1318:1331	wound dressing	1318:1331	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	2	18	theme	energy	539:544	arg1	EDX					564:566	EDX	564:566	EDX	564:566	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	18	theme	energy	539:544	arg1	X-Ray					557:561	energy dispersive X-Ray	539:561	energy dispersive X-Ray (EDX) analyses	539:576	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	4	19	theme	antibacterial	840:852	arg1	activity					854:861	antibacterial activity	840:861	antibacterial activity	840:861	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	7	20	theme	considerable	1162:1173	arg1	activities					1186:1195	considerable biological activities	1162:1195	considerable biological activities	1162:1195	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	1	21	theme	nanobiocomposite	192:207	arg1	scaffold					209:216	a nanobiocomposite scaffold	190:216	a nanobiocomposite scaffold	190:216	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	0	22	theme	hydrogel-polyvinyl	9:26	arg1	nanorods					69:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods	0:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	2	23	theme	scanning	425:432	arg1	FE-SEM					455:460	FE-SEM	455:460	FE-SEM	455:460	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	23	theme	scanning	425:432	arg1	microscope					443:452	field emission scanning electron microscope	410:452	field emission scanning electron microscope (FE-SEM)	410:461	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	0	24	theme	Alginate	0:7	arg1	nanorods					69:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods	0:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	1	25	theme	magnesium	299:307	arg1	nanorods					319:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	6	26	theme	cells	1139:1143	arg1	cells					1139:1143	cells	1139:1143	cells	1139:1143	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	6	26	theme	cells	1139:1143	arg1	%					1134:1134	about 92%	1126:1134	about 92% of cells	1126:1143	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	2	27	theme	emission	416:423	arg1	FE-SEM					455:460	FE-SEM	455:460	FE-SEM	455:460	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	27	theme	emission	416:423	arg1	microscope					443:452	field emission scanning electron microscope	410:452	field emission scanning electron microscope (FE-SEM)	410:461	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	0	28	theme	fibroin/magnesium	41:57	arg1	nanorods					69:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods	0:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	1	29	theme	hydroxide	309:317	arg1	nanorods					319:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	0	30	theme	mechanical	152:161	arg1	properties					163:172	improved mechanical properties	143:172	improved mechanical properties	143:172	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	2	31	theme	structural	333:342	arg1	characteristics					344:358	The structural characteristics	329:358	The structural characteristics	329:358	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	6	32	theme	hemolytic	1088:1096	arg1	%					1120:1120	less than 8%	1109:1120	less than 8%	1109:1120	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	6	32	theme	hemolytic	1088:1096	arg1	effect					1098:1103	the hemolytic effect	1084:1103	the hemolytic effect	1084:1103	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	0	33	theme	alcohol/silk	28:39	arg1	nanorods					69:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods	0:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	0	34	theme	improved	143:150	arg1	properties					163:172	improved mechanical properties	143:172	improved mechanical properties	143:172	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	3	35	theme	scaffold	680:687	arg1	properties					638:647	mechanical properties	627:647	mechanical properties	627:647	To introduce the application, biocompatibility, mechanical properties and biological activity of the scaffold were obtained.					
32814101	3	35	theme	scaffold	680:687	arg1	activity					664:671	biological activity	653:671	biological activity	653:671	To introduce the application, biocompatibility, mechanical properties and biological activity of the scaffold were obtained.					
32814101	4	36	theme	excellent	768:776	arg1	adaptation					787:796	excellent cellular adaptation	768:796	excellent cellular adaptation	768:796	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	6	37	theme	nanobiocomposite	1055:1070	arg1	assay					1007:1011	Hemolytic assay	997:1011	Hemolytic assay	997:1011	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	6	37	theme	nanobiocomposite	1055:1070	arg1	activity					1032:1039	cell metabolic activity	1017:1039	cell metabolic activity	1017:1039	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	6	38	theme	novel	1049:1053	arg1	nanobiocomposite					1055:1070	this novel nanobiocomposite	1044:1070	this novel nanobiocomposite	1044:1070	Hemolytic assay and cell metabolic activity of this novel nanobiocomposite showed that the hemolytic effect was less than 8% and about 92% of cells survived.					
32814101	0	39	theme	hydroxide	59:67	arg1	nanorods					69:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods	0:76	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	2	40	theme	Fourier-transformed	498:516	arg1	FT-IR					528:532	FT-IR	528:532	FT-IR	528:532	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	40	theme	Fourier-transformed	498:516	arg1	infrared					518:525	Fourier-transformed infrared	498:525	Fourier-transformed infrared (FT-IR)	498:533	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	3	41	theme	mechanical	627:636	arg1	properties					638:647	mechanical properties	627:647	mechanical properties	627:647	To introduce the application, biocompatibility, mechanical properties and biological activity of the scaffold were obtained.					
32814101	1	42	theme	sodium	246:251	arg1	alginate					253:260	sodium alginate	246:260	sodium alginate	246:260	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	7	43	theme	acceptable	1201:1210	arg1	properties					1223:1232	acceptable mechanical properties	1201:1232	acceptable mechanical properties	1201:1232	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	0	44	dep	nanorods	69:76	arg1	scaffold					87:94	A novel scaffold	79:94	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	2	45	theme	scaffold	382:389	arg1	properties					364:373	properties	364:373	properties	364:373	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	45	theme	scaffold	382:389	arg1	characteristics					344:358	The structural characteristics	329:358	The structural characteristics	329:358	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	5	46	theme	scaffold	954:961	arg1	strength					963:970	the scaffold strength	950:970	the scaffold strength due to mechanical tests	950:994	In addition, silk fibroin and alginate increased the scaffold strength due to mechanical tests.					
32814101	7	47	theme	tissue	1334:1339	arg1	engineering					1341:1351	tissue engineering	1334:1351	tissue engineering	1334:1351	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	4	48	theme	high	736:739	arg1	porosity					741:748	high porosity	736:748	high porosity	736:748	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	7	49	theme	drug	1357:1360	arg1	systems					1371:1377	drug delivery systems	1357:1377	drug delivery systems	1357:1377	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	3	50	theme	biological	653:662	arg1	activity					664:671	biological activity	653:671	biological activity	653:671	To introduce the application, biocompatibility, mechanical properties and biological activity of the scaffold were obtained.					
32814101	4	51	theme	cellular	778:785	arg1	adaptation					787:796	excellent cellular adaptation	768:796	excellent cellular adaptation	768:796	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	1	52	theme	alginate	253:260	arg1	nanorods					319:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	7	53	theme	mechanical	1212:1221	arg1	properties					1223:1232	acceptable mechanical properties	1201:1232	acceptable mechanical properties	1201:1232	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	7	54	theme	delivery	1362:1369	arg1	systems					1371:1377	drug delivery systems	1357:1377	drug delivery systems	1357:1377	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	4	55	contain	had	836:838	arg1	nanorod					828:834	2 nanorod	826:834	2 nanorod	826:834	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	4	55	contain	had	836:838	arg2	activity					854:861	antibacterial activity	840:861	antibacterial activity	840:861	The composite was found to have high porosity, no cytotoxicity, excellent cellular adaptation, and most importantly Mg(OH)2 nanorod had antibacterial activity and inhibited the growth of bacteria.					
32814101	7	56	theme	possible	1302:1309	arg1	use					1311:1313	possible use	1302:1313	possible use in wound dressing, tissue engineering and drug delivery systems	1302:1377	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	2	57	theme	field	410:414	arg1	FE-SEM					455:460	FE-SEM	455:460	FE-SEM	455:460	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	57	theme	field	410:414	arg1	microscope					443:452	field emission scanning electron microscope	410:452	field emission scanning electron microscope (FE-SEM)	410:461	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	58	theme	electron	434:441	arg1	FE-SEM					455:460	FE-SEM	455:460	FE-SEM	455:460	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	58	theme	electron	434:441	arg1	microscope					443:452	field emission scanning electron microscope	410:452	field emission scanning electron microscope (FE-SEM)	410:461	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	1	59	theme	polyvinyl	263:271	arg1	alcohol					273:279	polyvinyl alcohol	263:279	polyvinyl alcohol	263:279	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32814101	0	60	theme	novel	81:85	arg1	scaffold					87:94	A novel scaffold	79:94	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.	0:173	Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: A novel scaffold with biological and antibacterial activity and improved mechanical properties.					
32814101	7	61	theme	biological	1175:1184	arg1	activities					1186:1195	considerable biological activities	1162:1195	considerable biological activities	1162:1195	Due to considerable biological activities and acceptable mechanical properties, the mentioned nanobiocomposite can be considered as a scaffold for possible use in wound dressing, tissue engineering and drug delivery systems.					
32814101	2	62	theme	thermogravimetric	464:480	arg1	TGA					492:494	TGA	492:494	TGA	492:494	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	2	62	theme	thermogravimetric	464:480	arg1	analysis					482:489	thermogravimetric analysis	464:489	thermogravimetric analysis (TGA)	464:495	The structural characteristics and properties of the scaffold were identified by field emission scanning electron microscope (FE-SEM), thermogravimetric analysis (TGA), Fourier-transformed infrared (FT-IR) and energy dispersive X-Ray (EDX) analyses.					
32814101	1	63	theme	alcohol	273:279	arg1	nanorods					319:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods	246:326	In this study, a nanobiocomposite scaffold was fabricated by combining sodium alginate, polyvinyl alcohol, silk fibroin and magnesium hydroxide nanorods.					
32998137	1	0	theme	mutans	392:397	arg1	biofilms					366:373	mixed biofilms	360:373	mixed biofilms of Streptococcus mutans and Candida albicans	360:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	4	1	theme	F	785:785	arg1	concentrations					767:780	the concentrations	763:780	the concentrations of F, Ca, and P	763:796	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	5	2	dep	solution	943:950	arg1	simulate					955:962	simulate	955:962	to simulate a cariogenic challenge	952:985	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	9	3	theme	biofilm	1420:1426	arg1	values					1406:1411	the highest pH values	1391:1411	the highest pH values of the biofilm	1391:1426	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	5	4	theme	%	933:933	arg1	solution					943:950	a 20% sucrose solution	929:950	a 20% sucrose solution to simulate a cariogenic challenge	929:985	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	0	5	from	Effects	0:6	arg1	Composition					76:86	Composition	76:86	Composition	76:86	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	0	5	from	Effects	0:6	arg1	pH					92:93	pH	92:93	pH	92:93	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	3	6	contain	containing	645:654	arg1	controls					725:732	controls	725:732	controls	725:732	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	3	6	contain	containing	645:654	arg1	Solutions					635:643	Solutions	635:643	Solutions containing 500 and 1,100 ppm F as well as artificial saliva	635:703	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	3	6	contain	containing	645:654	arg2	F					674:674	500 and 1,100 ppm F	656:674	F	674:674	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	3	6	contain	containing	645:654	arg1	saliva					698:703	artificial saliva	687:703	Solutions containing 500 and 1,100 ppm F as well as artificial saliva	635:703	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	4	7	theme	Ca	788:789	arg1	concentrations					767:780	the concentrations	763:780	the concentrations of F, Ca, and P	763:796	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	10	8	theme	medium	1596:1601	arg1	pH					1586:1587	the pH	1582:1587	the pH of the medium	1582:1601	It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.					
32998137	7	9	with	Treatment	1157:1165	arg1	TMP					1172:1174	TMP	1172:1174	TMP	1172:1174	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	7	9	with	Treatment	1157:1165	arg1	ppm					1184:1186	500 ppm	1180:1186	500 ppm	1180:1186	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	1	10	theme	phosphorus	328:337	arg1	concentrations					289:302	the concentrations	285:302	the concentrations of F, calcium (Ca), and phosphorus (P)	285:341	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	5	11	theme	cariogenic	966:975	arg1	challenge					977:985	a cariogenic challenge	964:985	a cariogenic challenge	964:985	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	3	12	theme	1,100	664:668	arg1	F					674:674	500 and 1,100 ppm F	656:674	F	674:674	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	1	13	theme	sodium	214:219	arg1	TMP					239:241	TMP	239:241	TMP	239:241	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	1	13	theme	sodium	214:219	arg1	trimetaphosphate					221:236	sodium trimetaphosphate	214:236	sodium trimetaphosphate (TMP)	214:242	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	6	14	theme	p	1146:1146	arg1	test					1140:1143	Fisher's least significant difference test	1102:1143	Fisher's least significant difference test (p < 0.05)	1102:1154	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	6	14	theme	p	1146:1146	arg1	<					1148:1148	p < 0.05	1146:1153	p < 0.05	1146:1153	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	7	15	theme	biofilm	1227:1233	arg1	fluid					1235:1239	the biofilm fluid	1223:1239	the biofilm fluid	1223:1239	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	0	16	dep	Composition	76:86	arg1	the					72:74	the	72:74	the	72:74	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	1	17	theme	Candida	403:409	arg1	albicans					411:418	Candida albicans	403:418	Candida albicans	403:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	4	18	dep	determined	803:812	arg1	phases					831:836	solid and fluid phases	815:836	solid and fluid phases	815:836	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	10	19	from	increase	1570:1577	arg1	pH					1586:1587	the pH	1582:1587	the pH of the medium	1582:1601	It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.					
32998137	1	20	theme	albicans	411:418	arg1	biofilms					366:373	mixed biofilms	360:373	mixed biofilms of Streptococcus mutans and Candida albicans	360:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	7	21	theme	higher	1197:1202	arg1	concentration					1206:1218	higher F concentration	1197:1218	higher F concentration in the biofilm fluid	1197:1239	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	7	22	theme	F	1204:1204	arg1	concentration					1206:1218	higher F concentration	1197:1218	higher F concentration in the biofilm fluid	1197:1239	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	6	23	theme	difference	1129:1138	arg1	test					1140:1143	Fisher's least significant difference test	1102:1143	Fisher's least significant difference test (p < 0.05)	1102:1154	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	6	23	theme	difference	1129:1138	arg1	<					1148:1148	p < 0.05	1146:1153	p < 0.05	1146:1153	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	9	24	theme	1	1371:1371	arg1	%					1372:1372	%	1372:1372	%	1372:1372	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	1	25	theme	trimetaphosphate	221:236	arg1	influence					201:209	the influence	197:209	the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans	197:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	7	26	from	concentration	1206:1218	arg1	fluid					1235:1239	the biofilm fluid	1223:1239	the biofilm fluid	1223:1239	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	4	27	theme	solid	815:819	arg1	phases					831:836	solid and fluid phases	815:836	solid and fluid phases	815:836	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	0	28	theme	Trimetaphosphate	18:33	arg1	Effects					0:6	Effects	0:6	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose	0:149	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	0	29	theme	Mixed	98:102	arg1	Biofilms					104:111	Mixed Biofilms	98:111	Mixed Biofilms	98:111	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	9	30	theme	%	1372:1372	arg1	TMP					1374:1376	1% TMP	1371:1376	1% TMP	1371:1376	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	8	31	theme	P	1338:1338	arg1	concentrations					1340:1353	higher P concentrations	1331:1353	higher P concentrations	1331:1353	Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations.					
32998137	0	32	theme	Sodium	11:16	arg1	Trimetaphosphate					18:33	Sodium Trimetaphosphate	11:33	Sodium Trimetaphosphate	11:33	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	5	33	theme	third	876:880	arg1	h					896:896	96 h	893:896	96 h	893:896	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	5	33	theme	third	876:880	arg1	treatment					882:890	the third treatment	872:890	the third treatment (96 h)	872:897	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	8	34	theme	higher	1331:1336	arg1	concentrations					1340:1353	higher P concentrations	1331:1353	higher P concentrations	1331:1353	Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations.					
32998137	8	35	theme	Ca	1270:1271	arg1	concentrations					1273:1286	Ca concentrations	1270:1286	Ca concentrations	1270:1286	Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations.					
32998137	4	36	theme	fluid	825:829	arg1	phases					831:836	solid and fluid phases	815:836	solid and fluid phases	815:836	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	6	37	theme	significant	1117:1127	arg1	test					1140:1143	Fisher's least significant difference test	1102:1143	Fisher's least significant difference test (p < 0.05)	1102:1154	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	6	37	theme	significant	1117:1127	arg1	<					1148:1148	p < 0.05	1146:1153	p < 0.05	1146:1153	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	2	38	theme	TMP	566:568	arg1	concentrations					570:583	three TMP concentrations	560:583	three TMP concentrations (0.25, 0.5, or 1%)	560:602	The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm.					
32998137	2	38	theme	TMP	566:568	arg1	%					601:601	0.25, 0.5, or 1%	586:601	0.25, 0.5, or 1%	586:601	The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm.					
32998137	4	39	theme	Biofilm	735:741	arg1	pH					743:744	Biofilm pH	735:744	Biofilm pH	735:744	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	1	40	from	influence	201:209	arg1	concentrations					289:302	the concentrations	285:302	the concentrations of F, calcium (Ca), and phosphorus (P)	285:341	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	1	40	from	influence	201:209	arg1	pH					354:355	the pH	350:355	the pH of mixed biofilms of Streptococcus mutans and Candida albicans	350:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	1	41	theme	F	307:307	arg1	concentrations					289:302	the concentrations	285:302	the concentrations of F, calcium (Ca), and phosphorus (P)	285:341	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	2	42	dep	treatments	487:496	arg1	h					514:514	72, 78, and 96 h	499:514	72, 78, and 96 h after the beginning of their formation	499:553	The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm.					
32998137	6	43	dep	submitted	1062:1070	arg1	followed					1090:1097	followed	1090:1097	followed by Fisher's least significant difference test (p < 0.05)	1090:1154	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	1	44	theme	present	167:173	arg1	study					175:179	the present study	163:179	the present study	163:179	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	3	45	theme	ppm	670:672	arg1	F					674:674	500 and 1,100 ppm F	656:674	F	674:674	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	9	46	with	Treatment	1356:1364	arg1	F					1382:1382	F	1382:1382	F	1382:1382	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	9	46	with	Treatment	1356:1364	arg1	TMP					1374:1376	1% TMP	1371:1376	1% TMP	1371:1376	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	0	47	theme	Biofilms	104:111	arg1	Composition					76:86	Composition	76:86	Composition	76:86	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	0	47	theme	Biofilms	104:111	arg1	pH					92:93	pH	92:93	pH	92:93	Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.					
32998137	2	48	from	ppm	630:632	arg1	concentrations					570:583	three TMP concentrations	560:583	three TMP concentrations (0.25, 0.5, or 1%)	560:602	The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm.					
32998137	2	48	from	ppm	630:632	arg1	%					601:601	0.25, 0.5, or 1%	586:601	0.25, 0.5, or 1%	586:601	The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm.					
32998137	1	49	theme	calcium	310:316	arg1	concentrations					289:302	the concentrations	285:302	the concentrations of F, calcium (Ca), and phosphorus (P)	285:341	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	3	50	theme	artificial	687:696	arg1	Solutions					635:643	Solutions	635:643	Solutions containing 500 and 1,100 ppm F as well as artificial saliva	635:703	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	3	50	theme	artificial	687:696	arg1	controls					725:732	controls	725:732	controls	725:732	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	3	50	theme	artificial	687:696	arg1	saliva					698:703	artificial saliva	687:703	Solutions containing 500 and 1,100 ppm F as well as artificial saliva	635:703	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	7	51	dep	TMP	1172:1174	arg1	F					1188:1188	F	1188:1188	F	1188:1188	Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid.					
32998137	4	52	theme	P	796:796	arg1	concentrations					767:780	the concentrations	763:780	the concentrations of F, Ca, and P	763:796	Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases).					
32998137	1	53	theme	mixed	360:364	arg1	biofilms					366:373	mixed biofilms	360:373	mixed biofilms of Streptococcus mutans and Candida albicans	360:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	10	54	theme	cariogenic	1619:1628	arg1	challenge					1630:1638	the cariogenic challenge	1615:1638	the cariogenic challenge	1615:1638	It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.					
32998137	5	55	theme	sucrose	935:941	arg1	solution					943:950	a 20% sucrose solution	929:950	a 20% sucrose solution to simulate a cariogenic challenge	929:985	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	1	56	theme	study	175:179	arg1	aim					156:158	The aim	152:158	The aim of the present study	152:179	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	1	57	theme	biofilms	366:373	arg1	pH					354:355	the pH	350:355	the pH of mixed biofilms of Streptococcus mutans and Candida albicans	350:418	The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose.					
32998137	9	58	theme	cariogenic	1455:1464	arg1	challenge					1466:1474	the cariogenic challenge	1451:1474	the cariogenic challenge	1451:1474	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	9	59	theme	pH	1403:1404	arg1	values					1406:1411	the highest pH values	1391:1411	the highest pH values of the biofilm	1391:1426	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	3	60	theme	500	656:658	arg1	F					674:674	500 and 1,100 ppm F	656:674	F	674:674	Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls.					
32998137	5	61	theme	20	931:932	arg1	%					933:933	%	933:933	%	933:933	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	5	62	theme	medium	1005:1010	arg1	TMP					1027:1029	TMP	1027:1029	TMP	1027:1029	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	5	62	theme	medium	1005:1010	arg1	Ca					1016:1017	Ca	1016:1017	Ca	1016:1017	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	5	62	theme	medium	1005:1010	arg1	F					1013:1013	F	1013:1013	F	1013:1013	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	5	62	theme	medium	1005:1010	arg1	pH					995:996	the pH	991:996	the pH of the medium	991:1010	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	5	62	theme	medium	1005:1010	arg1	P					1020:1020	P	1020:1020	P	1020:1020	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
32998137	9	63	theme	highest	1395:1401	arg1	values					1406:1411	the highest pH values	1391:1411	the highest pH values of the biofilm	1391:1426	Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge.					
32998137	2	64	theme	formation	545:553	arg1	beginning					526:534	the beginning	522:534	the beginning of their formation	522:553	The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm.					
32998137	6	65	theme	two-way	1075:1081	arg1	ANOVA					1083:1087	two-way ANOVA	1075:1087	two-way ANOVA	1075:1087	The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05).					
32998137	5	66	theme	parallel	845:852	arg1	experiment					854:863	a parallel experiment	843:863	a parallel experiment	843:863	In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined.					
34353248	0	0	theme	Lactose	85:91	arg1	Additives					93:101	Glucose and Lactose Additives	73:101	Glucose and Lactose Additives	73:101	Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.					
34353248	10	1	theme	E.	1630:1631	arg1	production					1664:1673	E. coli-based recombinant protein production	1630:1673	E. coli-based recombinant protein production	1630:1673	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	8	2	theme	digest	1129:1134	arg1	type					1113:1116	the type	1109:1116	the type of protein digest	1109:1134	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	6	3	theme	additive-containing	780:798	arg1	medium					800:805	the additive-containing medium	776:805	the additive-containing medium	776:805	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	1	4	theme	recombinant	161:171	arg1	proteins					173:180	recombinant proteins	161:180	recombinant proteins	161:180	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	9	5	theme	culture	1497:1503	arg1	liter					1488:1492	liter	1488:1492	liter of culture	1488:1503	The two recombinant proteins were produced in milligram quantity of purified protein per liter of culture.					
34353248	8	6	dep	successful	1278:1287	arg1	supplementing					1137:1149	supplementing	1137:1149	supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L)	1137:1222	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	0	7	from	Auto-Induction	9:22	arg1	coli					63:66	Escherichia coli	51:66	Escherichia coli	51:66	Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.					
34353248	8	8	theme	protein	1121:1127	arg1	digest					1129:1134	protein digest	1121:1134	protein digest	1121:1134	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	7	9	theme	Auto-induced	968:979	arg1	expression					987:996	Auto-induced GFPuv expression	968:996	Auto-induced GFPuv expression	968:996	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	10	10	theme	medium	1521:1526	arg1	useful					1581:1586	useful	1581:1586	useful	1581:1586	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	10	10	theme	medium	1521:1526	arg1	composition					1528:1538	The medium composition	1517:1538	The medium composition shown in this study	1517:1558	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	8	11	from	concentration	1248:1260	arg1	lactose					1228:1234	lactose	1228:1234	lactose (at a final concentration of 7.6 g/L)	1228:1272	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	6	12	dep	angiotensinogen	832:846	arg1	tac					849:851	tac	849:851	tac promoter-based expression	849:877	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	8	13	from	concentration	1198:1210	arg1	glucose					1178:1184	glucose	1178:1184	glucose (at a final concentration of 2.9 g/L)	1178:1222	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	6	14	theme	Thermus	884:890	arg1	expression					947:956	T7 lac promoter-based expression	925:956	T7 lac promoter-based expression	925:956	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	6	14	theme	Thermus	884:890	arg1	manganese-catalase					905:922	Thermus thermophilus manganese-catalase	884:922	Thermus thermophilus manganese-catalase (T7 lac promoter-based expression)	884:957	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	2	15	theme	culture	329:335	arg1	medium					337:342	a complex culture medium	319:342	a complex culture medium prepared by mixing culture substrates	319:380	Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates.					
34353248	6	16	dep	promoter-based	853:866	arg1	expression					868:877	expression	868:877	expression	868:877	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	8	17	theme	final	1242:1246	arg1	concentration					1248:1260	a final concentration	1240:1260	a final concentration of 7.6 g/L	1240:1271	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	8	18	theme	Broth	1160:1164	arg1	medium					1166:1171	Terrific Broth medium	1151:1171	Terrific Broth medium	1151:1171	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	8	19	theme	similar	1321:1327	arg1	level					1315:1319	an induction level	1302:1319	an induction level similar to that achieved with a commercially available auto-induction medium	1302:1396	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	2	20	theme	complex	321:327	arg1	medium					337:342	a complex culture medium	319:342	a complex culture medium prepared by mixing culture substrates	319:380	Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates.					
34353248	3	21	theme	culture	402:408	arg1	substrates					410:419	culture substrates	402:419	culture substrates	402:419	The differences in culture substrates sometimes lead to variations in the induction level.					
34353248	3	22	from	variations	439:448	arg1	level					467:471	the induction level	453:471	the induction level	453:471	The differences in culture substrates sometimes lead to variations in the induction level.					
34353248	5	23	theme	recombinant	688:698	arg1	expression					706:715	recombinant GFPuv expression	688:715	recombinant GFPuv expression	688:715	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	9	24	theme	purified	1467:1474	arg1	protein					1476:1482	purified protein	1467:1482	purified protein	1467:1482	The two recombinant proteins were produced in milligram quantity of purified protein per liter of culture.					
34353248	1	25	theme	inducer	190:196	arg1	addition					198:205	inducer addition	190:205	inducer addition using lac operon-controlled Escherichia coli expression systems	190:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	5	26	theme	GFPuv	700:704	arg1	expression					706:715	recombinant GFPuv expression	688:715	recombinant GFPuv expression	688:715	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	10	27	theme	recombinant	1644:1654	arg1	production					1664:1673	E. coli-based recombinant protein production	1630:1673	E. coli-based recombinant protein production	1630:1673	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	2	28	theme	culture	363:369	arg1	substrates					371:380	culture substrates	363:380	culture substrates	363:380	Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates.					
34353248	8	29	theme	final	1192:1196	arg1	concentration					1198:1210	a final concentration	1190:1210	a final concentration of 2.9 g/L	1190:1221	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	4	30	theme	culture	606:612	arg1	medium					614:619	a complex culture medium	596:619	a complex culture medium	596:619	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34353248	6	31	theme	promoter-based	853:866	arg1	tac					849:851	tac	849:851	tac promoter-based expression	849:877	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	6	32	dep	Thermus	884:890	arg1	thermophilus					892:903	thermophilus	892:903	thermophilus	892:903	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	8	33	theme	induction	1305:1313	arg1	level					1315:1319	an induction level	1302:1319	an induction level similar to that achieved with a commercially available auto-induction medium	1302:1396	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	0	34	theme	Recombinant	27:37	arg1	Proteins					39:46	Recombinant Proteins	27:46	Recombinant Proteins	27:46	Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.					
34353248	4	35	theme	complex	598:604	arg1	medium					614:619	a complex culture medium	596:619	a complex culture medium	596:619	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34353248	5	36	theme	auto-induction	637:650	arg1	levels					652:657	auto-induction levels	637:657	auto-induction levels	637:657	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	1	37	theme	BACKGROUND	104:113	arg1	way					146:148	a convenient way	133:148	a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems	133:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	1	37	theme	BACKGROUND	104:113	arg1	Auto-induction					115:128	BACKGROUND Auto-induction	104:128	BACKGROUND Auto-induction	104:128	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	1	38	theme	lac	213:215	arg1	systems					263:269	lac operon-controlled Escherichia coli expression systems	213:269	lac operon-controlled Escherichia coli expression systems	213:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	7	39	theme	digest	1078:1083	arg1	tryptone					1085:1092	another digest tryptone	1070:1092	another digest tryptone	1070:1092	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	10	40	theme	coli-based	1633:1642	arg1	production					1664:1673	E. coli-based recombinant protein production	1630:1673	E. coli-based recombinant protein production	1630:1673	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	1	41	theme	operon-controlled	217:233	arg1	systems					263:269	lac operon-controlled Escherichia coli expression systems	213:269	lac operon-controlled Escherichia coli expression systems	213:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	1	42	theme	Escherichia	235:245	arg1	systems					263:269	lac operon-controlled Escherichia coli expression systems	213:269	lac operon-controlled Escherichia coli expression systems	213:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	0	43	theme	Proteins	39:46	arg1	Auto-Induction					9:22	Auto-Induction	9:22	Auto-Induction of Recombinant Proteins in Escherichia coli	9:66	Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.					
34353248	4	44	theme	auto-induction	576:589	arg1	lactose					553:559	lactose	553:559	lactose	553:559	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34353248	4	44	theme	auto-induction	576:589	arg1	glucose					541:547	glucose	541:547	glucose	541:547	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34353248	4	44	theme	auto-induction	576:589	arg1	boosters					564:571	boosters	564:571	boosters of auto-induction with a complex culture medium	564:619	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34353248	10	45	dep	CONCLUSION	1506:1515	arg1	composition					1528:1538	The medium composition	1517:1538	The medium composition shown in this study	1517:1558	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	10	45	dep	CONCLUSION	1506:1515	arg1	useful					1581:1586	useful	1581:1586	useful	1581:1586	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	8	46	theme	g/L	1269:1271	arg1	concentration					1248:1260	a final concentration	1240:1260	a final concentration of 7.6 g/L	1240:1271	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	10	47	theme	protein	1656:1662	arg1	production					1664:1673	E. coli-based recombinant protein production	1630:1673	E. coli-based recombinant protein production	1630:1673	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	9	48	theme	recombinant	1407:1417	arg1	proteins					1419:1426	The two recombinant proteins	1399:1426	The two recombinant proteins	1399:1426	The two recombinant proteins were produced in milligram quantity of purified protein per liter of culture.					
34353248	4	49	with	boosters	564:571	arg1	medium					614:619	a complex culture medium	596:619	a complex culture medium	596:619	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34353248	3	50	theme	induction	457:465	arg1	level					467:471	the induction level	453:471	the induction level	453:471	The differences in culture substrates sometimes lead to variations in the induction level.					
34353248	9	51	theme	milligram	1445:1453	arg1	quantity					1455:1462	milligram quantity	1445:1462	milligram quantity of purified protein per liter of culture	1445:1503	The two recombinant proteins were produced in milligram quantity of purified protein per liter of culture.					
34353248	5	52	theme	T7	742:743	arg1	promoter					749:756	the T7 lac promoter	738:756	the T7 lac promoter	738:756	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	6	53	theme	promoter-based	932:945	arg1	expression					947:956	T7 lac promoter-based expression	925:956	T7 lac promoter-based expression	925:956	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	6	53	theme	promoter-based	932:945	arg1	manganese-catalase					905:922	Thermus thermophilus manganese-catalase	884:922	Thermus thermophilus manganese-catalase (T7 lac promoter-based expression)	884:957	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	8	54	theme	Terrific	1151:1158	arg1	medium					1166:1171	Terrific Broth medium	1151:1171	Terrific Broth medium	1151:1171	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	6	55	theme	ovine	826:830	arg1	angiotensinogen					832:846	ovine angiotensinogen	826:846	ovine angiotensinogen (tac promoter-based expression)	826:878	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	7	56	theme	protein	1030:1036	arg1	Polypepton					1045:1054	the enzymatic protein digest Polypepton	1016:1054	the enzymatic protein digest Polypepton	1016:1054	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	5	57	dep	assessed	664:671	arg1	First					630:634	First	630:634	First	630:634	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	7	58	theme	digest	1038:1043	arg1	Polypepton					1045:1054	the enzymatic protein digest Polypepton	1016:1054	the enzymatic protein digest Polypepton	1016:1054	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	5	59	theme	lac	745:747	arg1	promoter					749:756	the T7 lac promoter	738:756	the T7 lac promoter	738:756	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	6	60	theme	lac	928:930	arg1	expression					947:956	T7 lac promoter-based expression	925:956	T7 lac promoter-based expression	925:956	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	6	60	theme	lac	928:930	arg1	manganese-catalase					905:922	Thermus thermophilus manganese-catalase	884:922	Thermus thermophilus manganese-catalase (T7 lac promoter-based expression)	884:957	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	1	61	dep	Escherichia	235:245	arg1	coli					247:250	coli	247:250	coli	247:250	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	7	62	dep	RESULTS	960:966	arg1	observed					1002:1009	observed	1002:1009	was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone	998:1092	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	1	63	theme	convenient	135:144	arg1	way					146:148	a convenient way	133:148	a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems	133:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	1	63	theme	convenient	135:144	arg1	Auto-induction					115:128	BACKGROUND Auto-induction	104:128	BACKGROUND Auto-induction	104:128	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	7	64	theme	enzymatic	1020:1028	arg1	Polypepton					1045:1054	the enzymatic protein digest Polypepton	1016:1054	the enzymatic protein digest Polypepton	1016:1054	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	1	65	theme	expression	252:261	arg1	systems					263:269	lac operon-controlled Escherichia coli expression systems	213:269	lac operon-controlled Escherichia coli expression systems	213:269	BACKGROUND Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems.					
34353248	5	66	theme	promoter	749:756	arg1	control					727:733	the control	723:733	the control of the T7 lac promoter	723:756	METHODS First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter.					
34353248	6	67	theme	T7	925:926	arg1	expression					947:956	T7 lac promoter-based expression	925:956	T7 lac promoter-based expression	925:956	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	6	67	theme	T7	925:926	arg1	manganese-catalase					905:922	Thermus thermophilus manganese-catalase	884:922	Thermus thermophilus manganese-catalase (T7 lac promoter-based expression)	884:957	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	7	68	theme	GFPuv	981:985	arg1	expression					987:996	Auto-induced GFPuv expression	968:996	Auto-induced GFPuv expression	968:996	RESULTS Auto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone.					
34353248	8	69	theme	available	1366:1374	arg1	medium					1391:1396	a commercially available auto-induction medium	1351:1396	a commercially available auto-induction medium	1351:1396	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	0	70	theme	Glucose	73:79	arg1	Additives					93:101	Glucose and Lactose Additives	73:101	Glucose and Lactose Additives	73:101	Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.					
34353248	3	71	from	differences	387:397	arg1	substrates					410:419	culture substrates	402:419	culture substrates	402:419	The differences in culture substrates sometimes lead to variations in the induction level.					
34353248	10	72	theme	reliable	1602:1609	arg1	auto-induction					1611:1624	reliable auto-induction	1602:1624	reliable auto-induction for E. coli-based recombinant protein production	1602:1673	CONCLUSION The medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.					
34353248	9	73	theme	protein	1476:1482	arg1	quantity					1455:1462	milligram quantity	1445:1462	milligram quantity of purified protein per liter of culture	1445:1503	The two recombinant proteins were produced in milligram quantity of purified protein per liter of culture.					
34353248	8	74	theme	g/L	1219:1221	arg1	concentration					1198:1210	a final concentration	1190:1210	a final concentration of 2.9 g/L	1190:1221	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	6	75	theme	medium	800:805	arg1	Effectiveness					759:771	Effectiveness	759:771	Effectiveness of the additive-containing medium	759:805	Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).					
34353248	8	76	theme	auto-induction	1376:1389	arg1	medium					1391:1396	a commercially available auto-induction medium	1351:1396	a commercially available auto-induction medium	1351:1396	Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium.					
34353248	4	77	dep	OBJECTIVES	474:483	arg1	investigated					503:514	investigated	503:514	investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium	503:619	OBJECTIVES In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.					
34520780	10	0	theme	membranes	1314:1322	arg1	properties					1285:1294	The all-inclusive properties	1267:1294	The all-inclusive properties of the PVA/F-CMCS membranes	1267:1322	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	4	1	from	homogeneity	551:561	arg1	structure					626:634	the internal structure	613:634	the internal structure of the membranes	613:651	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	10	2	theme	potential	1338:1346	arg1	use					1348:1350	its potential use	1334:1350	its potential use in wound dressing applications	1334:1381	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	4	3	theme	internal	617:624	arg1	structure					626:634	the internal structure	613:634	the internal structure of the membranes	613:651	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	0	4	theme	wound	86:90	arg1	dressings					92:100	wound dressings	86:100	wound dressings	86:100	Fungal-derived carboxymethyl chitosan blended with polyvinyl alcohol as membranes for wound dressings.					
34520780	8	5	theme	developed	1111:1119	arg1	membranes					1121:1129	the developed membranes	1107:1129	the developed membranes	1107:1129	The hemolysis assay demonstrated the hemocompatibility of the developed membranes.					
34520780	8	6	theme	hemolysis	1053:1061	arg1	assay					1063:1067	The hemolysis assay	1049:1067	The hemolysis assay	1049:1067	The hemolysis assay demonstrated the hemocompatibility of the developed membranes.					
34520780	9	7	theme	biocompatibility	1151:1166	arg1	hemolysis					1178:1186	The antibacterial, biocompatibility, and good hemolysis	1132:1186	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane	1132:1206	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	8	8	theme	membranes	1121:1129	arg1	hemocompatibility					1086:1102	the hemocompatibility	1082:1102	the hemocompatibility of the developed membranes	1082:1129	The hemolysis assay demonstrated the hemocompatibility of the developed membranes.					
34520780	1	9	link	mushroom-derived	185:200	arg1	F-CMCS					226:231	F-CMCS	226:231	F-CMCS	226:231	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	1	9	link	mushroom-derived	185:200	arg1	chitosan					216:223	fungal mushroom-derived carboxymethyl chitosan	178:223	fungal mushroom-derived carboxymethyl chitosan (F-CMCS)	178:232	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	7	10	theme	cell	940:943	arg1	study					945:949	An in vitro cell study	928:949	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes	928:1015	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	1	11	theme	simple	256:261	arg1	technique					280:288	a simple solution casting technique	254:288	a simple solution casting technique for wound dressing applications	254:320	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	4	12	theme	emission	499:506	arg1	microscopy					526:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	9	13	theme	good	1173:1176	arg1	hemolysis					1178:1186	The antibacterial, biocompatibility, and good hemolysis	1132:1186	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane	1132:1206	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	1	14	theme	solution	263:270	arg1	technique					280:288	a simple solution casting technique	254:288	a simple solution casting technique for wound dressing applications	254:320	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	10	15	theme	PVA/F-CMCS	1303:1312	arg1	membranes					1314:1322	the PVA/F-CMCS membranes	1299:1322	the PVA/F-CMCS membranes	1299:1322	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	4	16	theme	Field	493:497	arg1	microscopy					526:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	0	17	link	Fungal-derived	0:13	arg1	chitosan					29:36	Fungal-derived carboxymethyl chitosan	0:36	Fungal-derived carboxymethyl chitosan	0:36	Fungal-derived carboxymethyl chitosan blended with polyvinyl alcohol as membranes for wound dressings.					
34520780	4	18	dep	homogeneity	551:561	arg1	the					547:549	the	547:549	the	547:549	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	7	19	theme	in	931:932	arg1	study					945:949	An in vitro cell study	928:949	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes	928:1015	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	10	20	theme	all-inclusive	1271:1283	arg1	properties					1285:1294	The all-inclusive properties	1267:1294	The all-inclusive properties of the PVA/F-CMCS membranes	1267:1322	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	4	21	theme	electron	517:524	arg1	microscopy					526:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	7	22	theme	PVA/F-CMCS	996:1005	arg1	membranes					1007:1015	the PVA/F-CMCS membranes	992:1015	the PVA/F-CMCS membranes	992:1015	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	6	23	dep	coli	862:865	arg1	gram-negative					868:880	gram-negative	868:880	gram-negative	868:880	The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria.					
34520780	2	24	theme	infrared	400:407	arg1	spectroscopy					409:420	Fourier infrared spectroscopy	392:420	Fourier infrared spectroscopy	392:420	The structural interactions between PVA and F-CMCS were confirmed by Fourier infrared spectroscopy.					
34520780	0	25	theme	carboxymethyl	15:27	arg1	chitosan					29:36	Fungal-derived carboxymethyl chitosan	0:36	Fungal-derived carboxymethyl chitosan	0:36	Fungal-derived carboxymethyl chitosan blended with polyvinyl alcohol as membranes for wound dressings.					
34520780	4	26	theme	scanning	508:515	arg1	microscopy					526:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy	493:535	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	4	27	theme	porous-like	590:600	arg1	network					602:608	a porous-like network	588:608	a porous-like network	588:608	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	10	28	from	use	1348:1350	arg1	applications					1370:1381	wound dressing applications	1355:1381	wound dressing applications	1355:1381	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	10	29	theme	dressing	1361:1368	arg1	applications					1370:1381	wound dressing applications	1355:1381	wound dressing applications	1355:1381	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	2	30	theme	Fourier	392:398	arg1	spectroscopy					409:420	Fourier infrared spectroscopy	392:420	Fourier infrared spectroscopy	392:420	The structural interactions between PVA and F-CMCS were confirmed by Fourier infrared spectroscopy.					
34520780	0	31	theme	Fungal-derived	0:13	arg1	chitosan					29:36	Fungal-derived carboxymethyl chitosan	0:36	Fungal-derived carboxymethyl chitosan	0:36	Fungal-derived carboxymethyl chitosan blended with polyvinyl alcohol as membranes for wound dressings.					
34520780	1	32	theme	casting	272:278	arg1	technique					280:288	a simple solution casting technique	254:288	a simple solution casting technique for wound dressing applications	254:320	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	1	33	theme	Multifunctional	103:117	arg1	membranes					125:133	Multifunctional blend membranes	103:133	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS)	103:232	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	3	34	theme	membranes	448:456	arg1	crystallinity					427:439	The crystallinity	423:439	The crystallinity of the membranes	423:456	The crystallinity of the membranes was examined by X-ray diffraction.					
34520780	1	35	theme	blend	119:123	arg1	membranes					125:133	Multifunctional blend membranes	103:133	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS)	103:232	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	9	36	theme	PVA	1195:1197	arg1	membrane					1199:1206	the PVA membrane	1191:1206	the PVA membrane	1191:1206	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	1	37	theme	mushroom-derived	185:200	arg1	F-CMCS					226:231	F-CMCS	226:231	F-CMCS	226:231	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	1	37	theme	mushroom-derived	185:200	arg1	chitosan					216:223	fungal mushroom-derived carboxymethyl chitosan	178:223	fungal mushroom-derived carboxymethyl chitosan (F-CMCS)	178:232	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	4	38	with	texture	575:581	arg1	network					602:608	a porous-like network	588:608	a porous-like network	588:608	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	7	39	from	study	945:949	arg1	membranes					1007:1015	the PVA/F-CMCS membranes	992:1015	the PVA/F-CMCS membranes	992:1015	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	9	40	dep	ratio	1250:1254	arg1	%					1264:1264	up to 40%	1256:1264	the F-CMCS composition ratio up to 40%	1227:1264	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	9	41	dep	composition	1238:1248	arg1	ratio					1250:1254	ratio	1250:1254	the F-CMCS composition ratio up to 40%	1227:1264	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	1	42	theme	carboxymethyl	202:214	arg1	F-CMCS					226:231	F-CMCS	226:231	F-CMCS	226:231	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	1	42	theme	carboxymethyl	202:214	arg1	chitosan					216:223	fungal mushroom-derived carboxymethyl chitosan	178:223	fungal mushroom-derived carboxymethyl chitosan (F-CMCS)	178:232	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	0	43	theme	polyvinyl	51:59	arg1	alcohol					61:67	polyvinyl alcohol	51:67	polyvinyl alcohol as membranes for wound dressings	51:100	Fungal-derived carboxymethyl chitosan blended with polyvinyl alcohol as membranes for wound dressings.					
34520780	1	44	theme	wound	294:298	arg1	applications					309:320	wound dressing applications	294:320	wound dressing applications	294:320	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	9	45	dep	40	1262:1263	arg1	to					1259:1260	to	1259:1260	to	1259:1260	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	1	46	theme	fungal	178:183	arg1	F-CMCS					226:231	F-CMCS	226:231	F-CMCS	226:231	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	1	46	theme	fungal	178:183	arg1	chitosan					216:223	fungal mushroom-derived carboxymethyl chitosan	178:223	fungal mushroom-derived carboxymethyl chitosan (F-CMCS)	178:232	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	4	47	from	texture	575:581	arg1	structure					626:634	the internal structure	613:634	the internal structure of the membranes	613:651	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	1	48	theme	dressing	300:307	arg1	applications					309:320	wound dressing applications	294:320	wound dressing applications	294:320	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	5	49	theme	F-CMCS	759:764	arg1	content					766:772	the F-CMCS content	755:772	the F-CMCS content	755:772	The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content.					
34520780	6	50	theme	antibacterial	819:831	arg1	activity					833:840	potential antibacterial activity	809:840	potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria	809:925	The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria.					
34520780	3	51	theme	X-ray	474:478	arg1	diffraction					480:490	X-ray diffraction	474:490	X-ray diffraction	474:490	The crystallinity of the membranes was examined by X-ray diffraction.					
34520780	5	52	dep	hydrophilicity	658:671	arg1	The					654:656	The	654:656	The	654:656	The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content.					
34520780	4	53	theme	coarser	567:573	arg1	texture					575:581	coarser texture	567:581	coarser texture	567:581	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	5	54	theme	fabricated	707:716	arg1	membranes					718:726	the fabricated membranes	703:726	the fabricated membranes	703:726	The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content.					
34520780	6	55	theme	potential	809:817	arg1	activity					833:840	potential antibacterial activity	809:840	potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria	809:925	The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria.					
34520780	4	56	with	homogeneity	551:561	arg1	network					602:608	a porous-like network	588:608	a porous-like network	588:608	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	5	57	theme	membranes	718:726	arg1	swelling					674:681	swelling	674:681	swelling	674:681	The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content.					
34520780	5	57	theme	membranes	718:726	arg1	degradation					688:698	degradation	688:698	degradation	688:698	The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content.					
34520780	5	57	theme	membranes	718:726	arg1	hydrophilicity					658:671	hydrophilicity	658:671	hydrophilicity	658:671	The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content.					
34520780	6	58	theme	gram-positive	903:915	arg1	bacteria					918:925	Staphylococcus (gram-positive) bacteria	887:925	Staphylococcus (gram-positive) bacteria	887:925	The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria.					
34520780	9	59	from	hemolysis	1178:1186	arg1	membrane					1199:1206	the PVA membrane	1191:1206	the PVA membrane	1191:1206	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	7	60	dep	in	931:932	arg1	vitro					934:938	vitro	934:938	vitro	934:938	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	7	61	theme	keratinocytes	975:987	arg1	study					945:949	An in vitro cell study	928:949	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes	928:1015	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	4	62	theme	membranes	643:651	arg1	structure					626:634	the internal structure	613:634	the internal structure of the membranes	613:651	Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes.					
34520780	9	63	theme	F-CMCS	1231:1236	arg1	composition					1238:1248	the F-CMCS composition ratio up to 40%	1227:1264	the F-CMCS composition ratio up to 40%	1227:1264	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
34520780	6	64	theme	PVA/F-CMCS	779:788	arg1	membrane					790:797	The PVA/F-CMCS membrane	775:797	The PVA/F-CMCS membrane	775:797	The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria.					
34520780	2	65	theme	structural	327:336	arg1	interactions					338:349	The structural interactions	323:349	The structural interactions between PVA and F-CMCS	323:372	The structural interactions between PVA and F-CMCS were confirmed by Fourier infrared spectroscopy.					
34520780	7	66	theme	skin	954:957	arg1	fibroblasts					959:969	skin fibroblasts	954:969	skin fibroblasts	954:969	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	6	67	theme	Staphylococcus	887:900	arg1	bacteria					918:925	Staphylococcus (gram-positive) bacteria	887:925	Staphylococcus (gram-positive) bacteria	887:925	The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria.					
34520780	10	68	theme	wound	1355:1359	arg1	applications					1370:1381	wound dressing applications	1355:1381	wound dressing applications	1355:1381	The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.					
34520780	1	69	theme	vinyl	153:157	arg1	poly					147:150	poly	147:150	poly (vinyl alcohol) (PVA)	147:172	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	1	69	theme	vinyl	153:157	arg1	alcohol					159:165	vinyl alcohol	153:165	vinyl alcohol	153:165	Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications.					
34520780	7	70	theme	fibroblasts	959:969	arg1	study					945:949	An in vitro cell study	928:949	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes	928:1015	An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility.					
34520780	9	71	theme	antibacterial	1136:1148	arg1	hemolysis					1178:1186	The antibacterial, biocompatibility, and good hemolysis	1132:1186	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane	1132:1206	The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%.					
33535983	6	0	theme	SSIIa	828:832	arg1	locus					834:838	SSIIa locus	828:838	SSIIa locus	828:838	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	6	1	theme	null	803:806	arg1	genotype					815:822	a triple null mutant genotype	794:822	a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger'	794:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	10	2	contain	had	1502:1504	arg1	mutations					1487:1495	The single individual genome mutations	1458:1495	The single individual genome mutations	1458:1495	The single individual genome mutations also had various effects on starch composition.					
33535983	10	2	contain	had	1502:1504	arg2	effects					1514:1520	various effects	1506:1520	various effects	1506:1520	The single individual genome mutations also had various effects on starch composition.					
33535983	5	3	theme	Starch	571:576	arg1	SSIIa					594:598	Starch synthase enzyme SSIIa	571:598	Starch synthase enzyme SSIIa	571:598	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	0	4	theme	Triple	0:5	arg1	mutations					12:20	Triple null mutations	0:20	Triple null mutations in starch synthase SSIIa gene homoeologs	0:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	1	5	theme	foods	170:174	arg1	Lack					136:139	BACKGROUND Lack	125:139	BACKGROUND Lack of nutritionally appropriate foods	125:174	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	11	6	theme	grain	1651:1655	arg1	phenotype					1657:1665	the shriveled grain phenotype	1637:1665	the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars	1637:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	5	7	theme	enzyme	587:592	arg1	SSIIa					594:598	Starch synthase enzyme SSIIa	571:598	Starch synthase enzyme SSIIa	571:598	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	6	8	theme	wheat	869:873	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	11	9	from	increase	1614:1621	arg1	RS					1626:1627	RS	1626:1627	RS	1626:1627	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	7	10	dep	RESULTS	1008:1014	arg1	identified					1097:1106	identified	1097:1106	were identified using TILLING	1092:1120	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	6	11	theme	red	858:860	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	8	12	theme	function	1151:1158	arg1	mutations					1160:1168	these loss-of function mutations	1137:1168	these loss-of function mutations on A, B, and D genomes	1137:1191	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	11	13	theme	other	1675:1679	arg1	knockouts					1687:1695	other ssiia knockouts	1675:1695	other ssiia knockouts in elite wheat cultivars	1675:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	1	14	theme	causes	198:203	arg1	one					179:181	one	179:181	one	179:181	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	1	14	theme	causes	198:203	arg1	causes					198:203	the leading causes	186:203	the leading causes of obesity	186:214	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	6	15	theme	elite	847:851	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	13	16	from	adoption	1906:1913	arg1	supply					1933:1938	supply	1933:1938	supply	1933:1938	Since all the mutants have been developed in an elite wheat cultivar, their adoption in production and supply will be feasible in future.					
33535983	13	16	from	adoption	1906:1913	arg1	production					1918:1927	production	1918:1927	production	1918:1927	Since all the mutants have been developed in an elite wheat cultivar, their adoption in production and supply will be feasible in future.					
33535983	5	17	theme	starch	615:620	arg1	SGP-1					649:653	SGP-1	649:653	SGP-1	649:653	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	5	17	theme	starch	615:620	arg1	isoform-1					638:646	starch granule protein isoform-1	615:646	starch granule protein isoform-1 (SGP-1)	615:654	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	5	18	theme	protein	630:636	arg1	SGP-1					649:653	SGP-1	649:653	SGP-1	649:653	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	5	18	theme	protein	630:636	arg1	isoform-1					638:646	starch granule protein isoform-1	615:646	starch granule protein isoform-1 (SGP-1)	615:654	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	0	19	from	amylose	76:82	arg1	wheat					118:122	hexaploid wheat	108:122	hexaploid wheat	108:122	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	9	20	from	increase	1381:1388	arg1	starch					1403:1408	resistant starch	1393:1408	resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat	1393:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	0	21	from	mutations	12:20	arg1	homoeologs					52:61	starch synthase SSIIa gene homoeologs	25:61	starch synthase SSIIa gene homoeologs	25:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	8	22	theme	D	1183:1183	arg1	genomes					1185:1191	A, B, and D genomes	1173:1191	A, B, and D genomes	1173:1191	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	1	23	theme	nutritionally	144:156	arg1	foods					170:174	nutritionally appropriate foods	144:174	nutritionally appropriate foods	144:174	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	13	24	theme	elite	1878:1882	arg1	cultivar					1890:1897	an elite wheat cultivar	1875:1897	an elite wheat cultivar	1875:1897	Since all the mutants have been developed in an elite wheat cultivar, their adoption in production and supply will be feasible in future.					
33535983	9	25	theme	%	1379:1379	arg1	increase					1381:1388	~ 118% increase	1374:1388	~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat	1374:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	7	26	theme	wheat	1069:1073	arg1	variety					1075:1081	wheat variety	1069:1081	wheat variety 'Jagger'	1069:1090	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	3	27	from	nutrients	333:341	arg1	grain					356:360	the wheat grain	346:360	the wheat grain	346:360	The nutrients in the wheat grain come primarily from the starch composed of amylose and amylopectin.					
33535983	0	28	theme	synthase	32:39	arg1	homoeologs					52:61	starch synthase SSIIa gene homoeologs	25:61	starch synthase SSIIa gene homoeologs	25:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	4	29	theme	Resistant	430:438	arg1	content					447:453	Resistant starch content	430:453	Resistant starch content	430:453	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	10	30	theme	genome	1480:1485	arg1	mutations					1487:1495	The single individual genome mutations	1458:1495	The single individual genome mutations	1458:1495	The single individual genome mutations also had various effects on starch composition.					
33535983	5	31	theme	polymer	736:742	arg1	amylopectin					744:754	the branched and readily digestible glucose polymer amylopectin	692:754	the branched and readily digestible glucose polymer amylopectin	692:754	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	6	32	theme	resistant	946:954	arg1	starch					956:961	resistant starch	946:961	resistant starch content in grains with respect to wild type	946:1005	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	4	33	theme	human	491:495	arg1	benefits					504:511	significant human health benefits	479:511	significant human health benefits	479:511	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	6	34	theme	mutations	933:941	arg1	effect					909:914	the effect	905:914	the effect of the knock-out mutations on resistant starch content in grains with respect to wild type	905:1005	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	8	35	theme	knockout	1240:1247	arg1	Jag-ssiia-∆ABD					1265:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	10	36	theme	various	1506:1512	arg1	effects					1514:1520	various effects	1506:1520	various effects	1506:1520	The single individual genome mutations also had various effects on starch composition.					
33535983	0	37	theme	starch	25:30	arg1	homoeologs					52:61	starch synthase SSIIa gene homoeologs	25:61	starch synthase SSIIa gene homoeologs	25:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	8	38	theme	genotype	1256:1263	arg1	Jag-ssiia-∆ABD					1265:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	11	39	theme	elite	1700:1704	arg1	cultivars					1712:1720	elite wheat cultivars	1700:1720	elite wheat cultivars	1700:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	5	40	theme	digestible	717:726	arg1	amylopectin					744:754	the branched and readily digestible glucose polymer amylopectin	692:754	the branched and readily digestible glucose polymer amylopectin	692:754	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	9	41	theme	Jagger	1444:1449	arg1	wheat					1451:1455	Jagger wheat	1444:1455	Jagger wheat	1444:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	7	42	dep	variety	1075:1081	arg1	Jagger					1084:1089	Jagger	1084:1089	wheat variety 'Jagger'	1069:1090	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	11	43	theme	Jag-ssiia-∆ABD	1571:1584	arg1	mutant					1586:1591	Our full null Jag-ssiia-∆ABD mutant	1557:1591	Our full null Jag-ssiia-∆ABD mutant	1557:1591	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	6	44	theme	wild	997:1000	arg1	type					1002:1005	wild type	997:1005	wild type	997:1005	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	11	45	theme	full	1561:1564	arg1	mutant					1586:1591	Our full null Jag-ssiia-∆ABD mutant	1557:1591	Our full null Jag-ssiia-∆ABD mutant	1557:1591	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	0	46	theme	gene	47:50	arg1	homoeologs					52:61	starch synthase SSIIa gene homoeologs	25:61	starch synthase SSIIa gene homoeologs	25:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	4	47	theme	synthesis	551:559	arg1	pathways					561:568	starch synthesis pathways	544:568	starch synthesis pathways	544:568	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	7	48	from	mutations	1026:1034	arg1	SSIIa					1039:1043	SSIIa	1039:1043	SSIIa	1039:1043	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	7	48	from	mutations	1026:1034	arg1	genomes					1058:1064	the three genomes	1048:1064	the three genomes of wheat variety 'Jagger'	1048:1090	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	13	49	from	future	1960:1965	arg1	feasible					1948:1955	feasible	1948:1955	feasible	1948:1955	Since all the mutants have been developed in an elite wheat cultivar, their adoption in production and supply will be feasible in future.					
33535983	4	50	contain	have	474:477	arg2	benefits					504:511	significant human health benefits	479:511	significant human health benefits	479:511	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	4	50	contain	have	474:477	arg1	content					447:453	Resistant starch content	430:453	Resistant starch content	430:453	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	6	51	theme	mutant	808:813	arg1	genotype					815:822	a triple null mutant genotype	794:822	a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger'	794:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	13	52	from	feasible	1948:1955	arg1	future					1960:1965	future	1960:1965	future	1960:1965	Since all the mutants have been developed in an elite wheat cultivar, their adoption in production and supply will be feasible in future.					
33535983	6	53	from	content	963:969	arg1	grains					974:979	grains	974:979	grains with respect to wild type	974:1005	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	1	54	theme	appropriate	158:168	arg1	foods					170:174	nutritionally appropriate foods	144:174	nutritionally appropriate foods	144:174	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	0	55	theme	SSIIa	41:45	arg1	homoeologs					52:61	starch synthase SSIIa gene homoeologs	25:61	starch synthase SSIIa gene homoeologs	25:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	6	56	theme	triple	796:801	arg1	genotype					815:822	a triple null mutant genotype	794:822	a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger'	794:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	9	57	from	%	1350:1350	arg1	Jagger					1355:1360	Jagger	1355:1360	Jagger	1355:1360	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	5	58	theme	synthase	578:585	arg1	SSIIa					594:598	Starch synthase enzyme SSIIa	571:598	Starch synthase enzyme SSIIa	571:598	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	9	59	theme	amylose	1307:1313	arg1	content					1315:1321	an amylose content	1304:1321	an amylose content of 35.70%	1304:1331	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	12	60	theme	non-GM	1813:1818	arg1	approach					1820:1827	a non-GM approach	1811:1827	a non-GM approach	1811:1827	Moreover, this study shows the potential for developing nutritionally improved foods in a non-GM approach.					
33535983	6	61	theme	variety	875:881	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	0	62	theme	hexaploid	108:116	arg1	wheat					118:122	hexaploid wheat	108:122	hexaploid wheat	108:122	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	6	63	theme	winter	862:867	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	2	64	dep	Triticum	248:255	arg1	aestivum					257:264	Triticum aestivum	248:264	Triticum aestivum	248:264	Wheat (Triticum aestivum) provides 20% of the calories consumed daily across the globe.					
33535983	10	65	theme	single	1462:1467	arg1	mutations					1487:1495	The single individual genome mutations	1458:1495	The single individual genome mutations	1458:1495	The single individual genome mutations also had various effects on starch composition.					
33535983	11	66	theme	wheat	1706:1710	arg1	cultivars					1712:1720	elite wheat cultivars	1700:1720	elite wheat cultivars	1700:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	9	67	theme	%	1331:1331	arg1	content					1315:1321	an amylose content	1304:1321	an amylose content of 35.70%	1304:1331	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	12	68	theme	improved	1793:1800	arg1	foods					1802:1806	nutritionally improved foods	1779:1806	nutritionally improved foods	1779:1806	Moreover, this study shows the potential for developing nutritionally improved foods in a non-GM approach.					
33535983	6	69	theme	hard	853:856	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	8	70	theme	loss-of	1143:1149	arg1	mutations					1160:1168	these loss-of function mutations	1137:1168	these loss-of function mutations on A, B, and D genomes	1137:1191	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	11	71	theme	ssiia	1681:1685	arg1	knockouts					1687:1695	other ssiia knockouts	1675:1695	other ssiia knockouts in elite wheat cultivars	1675:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	0	72	from	starch	98:103	arg1	wheat					118:122	hexaploid wheat	108:122	hexaploid wheat	108:122	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	5	73	theme	granule	622:628	arg1	SGP-1					649:653	SGP-1	649:653	SGP-1	649:653	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	5	73	theme	granule	622:628	arg1	isoform-1					638:646	starch granule protein isoform-1	615:646	starch granule protein isoform-1 (SGP-1)	615:654	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	1	74	dep	US	223:224	arg1	the					219:221	the	219:221	the	219:221	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	8	75	theme	A	1173:1173	arg1	genomes					1185:1191	A, B, and D genomes	1173:1191	A, B, and D genomes	1173:1191	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	11	76	theme	significant	1602:1612	arg1	increase					1614:1621	a significant increase	1600:1621	a significant increase in RS	1600:1627	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	1	77	from	one	179:181	arg1	worldwide					230:238	worldwide	230:238	worldwide	230:238	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	1	77	from	one	179:181	arg1	US					223:224	US	223:224	US	223:224	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	1	78	theme	BACKGROUND	125:134	arg1	Lack					136:139	BACKGROUND Lack	125:139	BACKGROUND Lack of nutritionally appropriate foods	125:174	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	9	79	from	starch	1403:1408	arg1	genotype					1432:1439	the Jag-ssiia-∆ABD genotype	1413:1439	the Jag-ssiia-∆ABD genotype of Jagger wheat	1413:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	8	80	theme	B	1176:1176	arg1	genomes					1185:1191	A, B, and D genomes	1173:1191	A, B, and D genomes	1173:1191	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	0	81	theme	high	71:74	arg1	amylose					76:82	high amylose	71:82	high amylose	71:82	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	9	82	contain	had	1300:1302	arg2	content					1315:1321	an amylose content	1304:1321	an amylose content of 35.70%	1304:1331	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	9	82	contain	had	1300:1302	arg1	Jag-ssiia-∆ABD					1285:1298	The Jag-ssiia-∆ABD	1281:1298	The Jag-ssiia-∆ABD	1281:1298	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	7	83	theme	variety	1075:1081	arg1	genomes					1058:1064	the three genomes	1048:1064	the three genomes of wheat variety 'Jagger'	1048:1090	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	9	84	theme	~ 118	1374:1378	arg1	%					1379:1379	%	1379:1379	%	1379:1379	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	6	85	from	effect	909:914	arg1	content					963:969	resistant starch content	946:969	resistant starch content in grains with respect to wild type	946:1005	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	13	86	theme	wheat	1884:1888	arg1	cultivar					1890:1897	an elite wheat cultivar	1875:1897	an elite wheat cultivar	1875:1897	Since all the mutants have been developed in an elite wheat cultivar, their adoption in production and supply will be feasible in future.					
33535983	8	87	from	mutations	1160:1168	arg1	genomes					1185:1191	A, B, and D genomes	1173:1191	A, B, and D genomes	1173:1191	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	4	88	theme	starch	440:445	arg1	content					447:453	Resistant starch content	430:453	Resistant starch content	430:453	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	11	89	from	knockouts	1687:1695	arg1	cultivars					1712:1720	elite wheat cultivars	1700:1720	elite wheat cultivars	1700:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	0	90	theme	null	7:10	arg1	mutations					12:20	Triple null mutations	0:20	Triple null mutations in starch synthase SSIIa gene homoeologs	0:61	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	11	91	theme	shriveled	1641:1649	arg1	phenotype					1657:1665	the shriveled grain phenotype	1637:1665	the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars	1637:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	9	92	theme	resistant	1393:1401	arg1	starch					1403:1408	resistant starch	1393:1408	resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat	1393:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	6	93	theme	starch	956:961	arg1	content					963:969	resistant starch content	946:969	resistant starch content in grains with respect to wild type	946:1005	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	4	94	theme	health	497:502	arg1	benefits					504:511	significant human health benefits	479:511	significant human health benefits	479:511	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	10	95	theme	individual	1469:1478	arg1	mutations					1487:1495	The single individual genome mutations	1458:1495	The single individual genome mutations	1458:1495	The single individual genome mutations also had various effects on starch composition.					
33535983	5	96	theme	amylopectin	744:754	arg1	biosynthesis					676:687	the biosynthesis	672:687	the biosynthesis of the branched and readily digestible glucose polymer amylopectin	672:754	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	5	97	theme	branched	696:703	arg1	amylopectin					744:754	the branched and readily digestible glucose polymer amylopectin	692:754	the branched and readily digestible glucose polymer amylopectin	692:754	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	1	98	theme	leading	190:196	arg1	causes					198:203	the leading causes	186:203	the leading causes of obesity	186:214	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	9	99	theme	Jag-ssiia-∆ABD	1417:1430	arg1	genotype					1432:1439	the Jag-ssiia-∆ABD genotype	1413:1439	the Jag-ssiia-∆ABD genotype of Jagger wheat	1413:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	6	100	theme	knock-out	923:931	arg1	mutations					933:941	the knock-out mutations	919:941	the knock-out mutations	919:941	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	8	101	theme	triple	1233:1238	arg1	Jag-ssiia-∆ABD					1265:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	0	102	theme	resistant	88:96	arg1	starch					98:103	resistant starch	88:103	resistant starch	88:103	Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.					
33535983	7	103	theme	Knock-out	1016:1024	arg1	mutations					1026:1034	Knock-out mutations	1016:1034	Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger'	1016:1090	RESULTS Knock-out mutations in SSIIa in the three genomes of wheat variety 'Jagger' were identified using TILLING.					
33535983	6	104	theme	work	774:777	arg1	goal					761:764	The goal	757:764	The goal of this work	757:777	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	3	105	theme	wheat	350:354	arg1	grain					356:360	the wheat grain	346:360	the wheat grain	346:360	The nutrients in the wheat grain come primarily from the starch composed of amylose and amylopectin.					
33535983	4	106	theme	significant	479:489	arg1	benefits					504:511	significant human health benefits	479:511	significant human health benefits	479:511	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	11	107	theme	null	1566:1569	arg1	mutant					1586:1591	Our full null Jag-ssiia-∆ABD mutant	1557:1591	Our full null Jag-ssiia-∆ABD mutant	1557:1591	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33535983	8	108	theme	mutant	1249:1254	arg1	Jag-ssiia-∆ABD					1265:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	a triple knockout mutant genotype Jag-ssiia-∆ABD	1231:1278	Subsequently, these loss-of function mutations on A, B, and D genomes were combined by crossing to generate a triple knockout mutant genotype Jag-ssiia-∆ABD.					
33535983	2	109	theme	calories	287:294	arg1	calories					287:294	the calories	283:294	the calories consumed daily across the globe	283:326	Wheat (Triticum aestivum) provides 20% of the calories consumed daily across the globe.					
33535983	2	109	theme	calories	287:294	arg1	%					278:278	20%	276:278	20% of the calories consumed daily across the globe	276:326	Wheat (Triticum aestivum) provides 20% of the calories consumed daily across the globe.					
33535983	9	110	theme	wheat	1451:1455	arg1	genotype					1432:1439	the Jag-ssiia-∆ABD genotype	1413:1439	the Jag-ssiia-∆ABD genotype of Jagger wheat	1413:1455	The Jag-ssiia-∆ABD had an amylose content of 35.70% compared to 31.15% in Jagger, leading to ~ 118% increase in resistant starch in the Jag-ssiia-∆ABD genotype of Jagger wheat.					
33535983	4	111	theme	starch	544:549	arg1	pathways					561:568	starch synthesis pathways	544:568	starch synthesis pathways	544:568	Resistant starch content, which is known to have significant human health benefits, can be increased by modifying starch synthesis pathways.					
33535983	10	112	theme	starch	1525:1530	arg1	composition					1532:1542	starch composition	1525:1542	starch composition	1525:1542	The single individual genome mutations also had various effects on starch composition.					
33535983	1	113	theme	obesity	208:214	arg1	causes					198:203	the leading causes	186:203	the leading causes of obesity	186:214	BACKGROUND Lack of nutritionally appropriate foods is one of the leading causes of obesity in the US and worldwide.					
33535983	6	114	from	genotype	815:822	arg1	Jagger					884:889	the elite hard red winter wheat variety 'Jagger'	843:890	the elite hard red winter wheat variety 'Jagger'	843:890	The goal of this work was to develop a triple null mutant genotype for SSIIa locus in the elite hard red winter wheat variety 'Jagger' and evaluate the effect of the knock-out mutations on resistant starch content in grains with respect to wild type.					
33535983	5	115	theme	glucose	728:734	arg1	amylopectin					744:754	the branched and readily digestible glucose polymer amylopectin	692:754	the branched and readily digestible glucose polymer amylopectin	692:754	Starch synthase enzyme SSIIa, also known as starch granule protein isoform-1 (SGP-1), is integral to the biosynthesis of the branched and readily digestible glucose polymer amylopectin.					
33535983	11	116	dep	CONCLUSIONS	1545:1555	arg1	showed					1593:1598	showed	1593:1598	showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars	1593:1720	CONCLUSIONS Our full null Jag-ssiia-∆ABD mutant showed a significant increase in RS without the shriveled grain phenotype seen in other ssiia knockouts in elite wheat cultivars.					
33444280	2	0	theme	low	339:341	arg1	bioavailability					348:362	low oral bioavailability	339:362	low oral bioavailability	339:362	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	1	1	theme	medicinal	254:262	arg1	herb					264:267	the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	242:297	the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	242:297	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	9	2	theme	rat	1506:1508	arg1	blood					1510:1514	rat blood	1506:1514	rat blood	1506:1514	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	4	3	theme	in	561:562	arg1	dissolution					570:580	in vitro dissolution	561:580	in vitro dissolution	561:580	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	4	4	theme	composite	625:633	arg1	dissolution					570:580	in vitro dissolution	561:580	in vitro dissolution	561:580	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	4	4	theme	composite	625:633	arg1	metabolism					594:603	in vivo metabolism	586:603	in vivo metabolism	586:603	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	6	5	theme	optimal	832:838	arg1	ratio					840:844	the optimal ratio	828:844	the optimal ratio of TA to drug carrier (WCS + γ-PGA)	828:880	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	4	6	dep	in	586:587	arg1	vivo					589:592	vivo	589:592	vivo	589:592	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	9	7	dep	times	1784:1788	arg1	that					1790:1793	that	1790:1793	that	1790:1793	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	8	theme	ultracentrifugation	1534:1552	arg1	test					1554:1557	an ultracentrifugation test	1531:1557	an ultracentrifugation test of an intra	1531:1569	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	7	9	dep	composite	1044:1052	arg1	ml-1					1075:1078	0.125 mg ml-1	1066:1078	the encapsulated composite using γ-PGA 0.125 mg ml-1	1027:1078	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	4	10	dep	in	561:562	arg1	vitro					564:568	vitro	564:568	vitro	564:568	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	3	11	theme	glutamic	478:485	arg1	WCS-γ-PGA					493:501	WCS-γ-PGA	493:501	WCS-γ-PGA	493:501	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	3	11	theme	glutamic	478:485	arg1	acid					487:490	glutamic acid	478:490	water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA)	442:502	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	4	12	from	metabolism	594:603	arg1	rats					638:641	rats	638:641	rats	638:641	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	9	13	theme	WCS-γ-PGA-TA	1729:1740	arg1	composite					1755:1763	the WCS-γ-PGA-TA encapsulated composite	1725:1763	the WCS-γ-PGA-TA encapsulated composite	1725:1763	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	7	14	theme	proper	977:982	arg1	ml-1					1090:1093	6 mg ml-1	1085:1093	6 mg ml-1	1085:1093	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	7	14	theme	proper	977:982	arg1	concentrations					984:997	The proper concentrations	973:997	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1	973:1078	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	9	15	dep	composite	1493:1501	arg1	measured					1519:1526	measured	1519:1526	measured by an ultracentrifugation test of an intra	1519:1569	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	16	theme	intra	1565:1569	arg1	test					1554:1557	an ultracentrifugation test	1531:1557	an ultracentrifugation test of an intra	1531:1569	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	17	theme	composite	1755:1763	arg1	times					1784:1788	4.56 and 4.20 times	1770:1788	4.56 and 4.20 times that of the original TA	1770:1812	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	17	theme	composite	1755:1763	arg1	area					1658:1661	the area	1654:1661	the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite	1654:1763	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	8	18	dep	in	1415:1416	arg1	vitro					1418:1422	vitro	1418:1422	vitro	1418:1422	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	9	19	theme	gastric	1573:1579	arg1	administration					1581:1594	gastric administration	1573:1594	gastric administration	1573:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	4	20	dep	dissolution	570:580	arg1	The					557:559	The	557:559	The	557:559	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	6	21	theme	room	933:936	arg1	temperature					938:948	room temperature	933:948	room temperature for the encapsulation	933:970	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	6	22	theme	h	928:928	arg1	time					918:921	a reaction time	907:921	a reaction time of 1 h at room temperature for the encapsulation	907:970	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	0	23	theme	in	109:110	arg1	properties					139:148	its in vitro/in vivo drug release properties	105:148	its in vitro/in vivo drug release properties	105:148	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	1	24	theme	Tanshinone	185:194	arg1	TA					201:202	TA	201:202	TA	201:202	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	1	24	theme	Tanshinone	185:194	arg1	IIA					196:198	Tanshinone IIA	185:198	Tanshinone IIA (TA)	185:203	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	1	24	theme	Tanshinone	185:194	arg1	component					227:235	an isolated component	215:235	an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	215:297	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	6	25	from	temperature	938:948	arg1	time					918:921	a reaction time	907:921	a reaction time of 1 h at room temperature for the encapsulation	907:970	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	6	25	from	temperature	938:948	arg1	h					928:928	1 h	926:928	1 h at room temperature for the encapsulation	926:970	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	7	26	theme	mg	1101:1102	arg1	ml-1					1104:1107	1 mg ml-1	1099:1107	1 mg ml-1	1099:1107	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	6	27	theme	reaction	909:916	arg1	time					918:921	a reaction time	907:921	a reaction time of 1 h at room temperature for the encapsulation	907:970	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	9	28	theme	original	1623:1630	arg1	concentration					1635:1647	the original TA concentration	1619:1647	the original TA concentration	1619:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	6	29	from	time	918:921	arg1	temperature					938:948	room temperature	933:948	room temperature for the encapsulation	933:970	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	9	30	theme	TA	1811:1812	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	30	theme	TA	1811:1812	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	30	theme	TA	1811:1812	arg1	times					1784:1788	4.56 and 4.20 times	1770:1788	4.56 and 4.20 times that of the original TA	1770:1812	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	30	theme	TA	1811:1812	arg1	area					1658:1661	the area	1654:1661	the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite	1654:1763	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	2	31	theme	clinical	382:389	arg1	application					391:401	its clinical application	378:401	its clinical application	378:401	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	0	32	theme	release	131:137	arg1	properties					139:148	its in vitro/in vivo drug release properties	105:148	its in vitro/in vivo drug release properties	105:148	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	0	33	theme	IIA	73:75	arg1	composite					91:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	9	34	from	administration	1581:1594	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	34	from	administration	1581:1594	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	35	theme	concentration	1635:1647	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	35	theme	concentration	1635:1647	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	35	theme	concentration	1635:1647	arg1	times					1784:1788	4.56 and 4.20 times	1770:1788	4.56 and 4.20 times that of the original TA	1770:1812	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	35	theme	concentration	1635:1647	arg1	area					1658:1661	the area	1654:1661	the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite	1654:1763	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	8	36	theme	that	1391:1394	arg1	times					1382:1386	5.56 times	1377:1386	5.56 times of that of the original TA in vitro dissolution	1377:1434	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	7	37	theme	mg	1087:1088	arg1	ml-1					1090:1093	6 mg ml-1	1085:1093	6 mg ml-1	1085:1093	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	7	37	theme	mg	1087:1088	arg1	concentrations					984:997	The proper concentrations	973:997	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1	973:1078	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	0	38	dep	in	109:110	arg1	vivo					121:124	vivo	121:124	vivo	121:124	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	0	39	theme	water-soluble	15:27	arg1	composite					91:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	8	40	theme	TA	1412:1413	arg1	dissolution					1424:1434	the original TA in vitro dissolution	1399:1434	the original TA in vitro dissolution	1399:1434	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	7	41	theme	mg	1072:1073	arg1	ml-1					1075:1078	0.125 mg ml-1	1066:1078	the encapsulated composite using γ-PGA 0.125 mg ml-1	1027:1078	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	0	42	theme	acid-tanshinone	57:71	arg1	composite					91:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	9	43	theme	concentration	1446:1458	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	43	theme	concentration	1446:1458	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	7	44	theme	composite	1197:1205	arg1	efficiency					1142:1151	encapsulation efficiency	1128:1151	encapsulation efficiency	1128:1151	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	7	44	theme	composite	1197:1205	arg1	efficiency					1170:1179	drug loading efficiency	1157:1179	drug loading efficiency	1157:1179	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	8	45	theme	TA	1289:1290	arg1	release					1278:1284	The cumulative release	1263:1284	The cumulative release of TA from the WCS-γ-PGA-TA	1263:1312	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	5	46	theme	encapsulation	761:773	arg1	validity					749:756	the validity	745:756	the validity of encapsulation for TA	745:780	FTIR spectroscopy was applied to confirm the validity of encapsulation for TA by WCS-γ-PGA.					
33444280	9	47	theme	curve	1683:1687	arg1	0-t					1694:1696	0-t	1694:1696	0-t	1694:1696	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	47	theme	curve	1683:1687	arg1	AUC					1689:1691	the drug-time curve AUC	1669:1691	the drug-time curve AUC (0-t)	1669:1697	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	2	48	theme	water	318:322	arg1	solubility					324:333	the poor water solubility	309:333	the poor water solubility	309:333	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	1	49	theme	isolated	218:225	arg1	IIA					196:198	Tanshinone IIA	185:198	Tanshinone IIA (TA)	185:203	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	1	49	theme	isolated	218:225	arg1	component					227:235	an isolated component	215:235	an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	215:297	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	7	50	theme	loading	1162:1168	arg1	efficiency					1170:1179	drug loading efficiency	1157:1179	drug loading efficiency	1157:1179	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	9	51	theme	encapsulated	1480:1491	arg1	composite					1493:1501	the encapsulated composite	1476:1501	the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra	1476:1569	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	2	52	theme	oral	343:346	arg1	bioavailability					348:362	low oral bioavailability	339:362	low oral bioavailability	339:362	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	1	53	theme	Chinese	246:252	arg1	herb					264:267	the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	242:297	the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	242:297	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	6	54	theme	drug	855:858	arg1	WCS + γ-PGA					869:879	WCS + γ-PGA	869:879	WCS + γ-PGA	869:879	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	6	54	theme	drug	855:858	arg1	carrier					860:866	drug carrier	855:866	drug carrier (WCS + γ-PGA)	855:880	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	7	55	theme	encapsulation	1128:1140	arg1	efficiency					1142:1151	encapsulation efficiency	1128:1151	encapsulation efficiency	1128:1151	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	10	56	theme	TA	1868:1869	arg1	encapsulation					1851:1863	the encapsulation	1847:1863	the encapsulation of TA with WCS-γ-PGA	1847:1884	It indicated that the encapsulation of TA with WCS-γ-PGA improved its solubility and bioavailability significantly.					
33444280	6	57	theme	TA	849:850	arg1	ratio					840:844	the optimal ratio	828:844	the optimal ratio of TA to drug carrier (WCS + γ-PGA)	828:880	The study's results showed that the optimal ratio of TA to drug carrier (WCS + γ-PGA) was 1:5.5 in weight with a reaction time of 1 h at room temperature for the encapsulation.					
33444280	0	58	theme	encapsulation	77:89	arg1	composite					91:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	4	59	theme	encapsulated	612:623	arg1	composite					625:633	the encapsulated composite	608:633	the encapsulated composite	608:633	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	1	60	from	herb	264:267	arg1	IIA					196:198	Tanshinone IIA	185:198	Tanshinone IIA (TA)	185:203	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	1	60	from	herb	264:267	arg1	component					227:235	an isolated component	215:235	an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza)	215:297	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	8	61	from	WCS-γ-PGA-TA	1301:1312	arg1	release					1278:1284	The cumulative release	1263:1284	The cumulative release of TA from the WCS-γ-PGA-TA	1263:1312	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	3	62	theme	oral	535:538	arg1	bioavailability					540:554	oral bioavailability	535:554	oral bioavailability	535:554	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	10	63	with	encapsulation	1851:1863	arg1	WCS-γ-PGA					1876:1884	WCS-γ-PGA	1876:1884	WCS-γ-PGA	1876:1884	It indicated that the encapsulation of TA with WCS-γ-PGA improved its solubility and bioavailability significantly.					
33444280	9	64	theme	encapsulated	1742:1753	arg1	composite					1755:1763	the WCS-γ-PGA-TA encapsulated composite	1725:1763	the WCS-γ-PGA-TA encapsulated composite	1725:1763	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	65	from	composite	1493:1501	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	65	from	composite	1493:1501	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	65	from	composite	1493:1501	arg1	blood					1510:1514	rat blood	1506:1514	rat blood	1506:1514	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	1	66	dep	herb	264:267	arg1	miltiorrhiza					285:296	miltiorrhiza	285:296	miltiorrhiza	285:296	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	1	66	dep	herb	264:267	arg1	Tanshen					269:275	Tanshen	269:275	Tanshen	269:275	Some diseases could be treated by Tanshinone IIA (TA), which is an isolated component from the Chinese medicinal herb Tanshen (Salvia miltiorrhiza).					
33444280	3	67	theme	soluble	450:456	arg1	chitosan/poly					458:470	soluble chitosan/poly	450:470	soluble chitosan/poly	450:470	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	4	68	from	dissolution	570:580	arg1	rats					638:641	rats	638:641	rats	638:641	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	0	69	theme	drug	126:129	arg1	properties					139:148	its in vitro/in vivo drug release properties	105:148	its in vitro/in vivo drug release properties	105:148	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	9	70	theme	original	1802:1809	arg1	TA					1811:1812	the original TA	1798:1812	the original TA	1798:1812	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	5	71	theme	FTIR	704:707	arg1	spectroscopy					709:720	FTIR spectroscopy	704:720	FTIR spectroscopy	704:720	FTIR spectroscopy was applied to confirm the validity of encapsulation for TA by WCS-γ-PGA.					
33444280	9	72	theme	TA	1632:1633	arg1	concentration					1635:1647	the original TA concentration	1619:1647	the original TA concentration	1619:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	8	73	theme	original	1403:1410	arg1	dissolution					1424:1434	the original TA in vitro dissolution	1399:1434	the original TA in vitro dissolution	1399:1434	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	4	74	theme	improvement	691:701	arg1	efficiency					673:682	the efficiency	669:682	the efficiency of the improvement	669:701	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33444280	2	75	theme	TA	367:368	arg1	solubility					324:333	the poor water solubility	309:333	the poor water solubility	309:333	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	2	75	theme	TA	367:368	arg1	bioavailability					348:362	low oral bioavailability	339:362	low oral bioavailability	339:362	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	0	76	theme	chitosan/poly-gama-glutamic	29:55	arg1	composite					91:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	15:99	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	7	77	dep	efficiency	1142:1151	arg1	The					1124:1126	The	1124:1126	The	1124:1126	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	8	78	theme	in	1415:1416	arg1	dissolution					1424:1434	the original TA in vitro dissolution	1399:1434	the original TA in vitro dissolution	1399:1434	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	7	79	theme	0.125	1066:1070	arg1	mg					1072:1073	mg	1072:1073	mg	1072:1073	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	9	80	theme	peak	1441:1444	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	80	theme	peak	1441:1444	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	7	81	theme	WCS-γ-PGA-TA	1184:1195	arg1	composite					1197:1205	WCS-γ-PGA-TA composite	1184:1205	WCS-γ-PGA-TA composite	1184:1205	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	8	82	theme	cumulative	1267:1276	arg1	release					1278:1284	The cumulative release	1263:1284	The cumulative release of TA from the WCS-γ-PGA-TA	1263:1312	The cumulative release of TA from the WCS-γ-PGA-TA encapsulated composite reached to 81% within 60 min, which was 5.56 times of that of the original TA in vitro dissolution.					
33444280	0	83	theme	composite	91:99	arg1	Preparation					0:10	Preparation	0:10	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite	0:99	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	0	83	theme	composite	91:99	arg1	properties					139:148	its in vitro/in vivo drug release properties	105:148	its in vitro/in vivo drug release properties	105:148	Preparation of water-soluble chitosan/poly-gama-glutamic acid-tanshinone IIA encapsulation composite and its in vitro/in vivo drug release properties.					
33444280	9	84	theme	drug-time	1673:1681	arg1	0-t					1694:1696	0-t	1694:1696	0-t	1694:1696	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	84	theme	drug-time	1673:1681	arg1	AUC					1689:1691	the drug-time curve AUC	1669:1691	the drug-time curve AUC (0-t)	1669:1697	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	85	theme	TA	1468:1469	arg1	Cmax					1460:1463	The peak concentration Cmax	1437:1463	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration	1437:1594	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	9	85	theme	TA	1468:1469	arg1	times					1605:1609	4.43 times	1600:1609	4.43 times that of the original TA concentration	1600:1647	The peak concentration Cmax of TA from the encapsulated composite in rat blood as measured by an ultracentrifugation test of an intra - gastric administration was 4.43 times that of the original TA concentration, and the area under the drug-time curve AUC (0-t) and AUC (0-∞) (p<0.01) of the WCS-γ-PGA-TA encapsulated composite were 4.56 and 4.20 times that of the original TA, respectively.					
33444280	7	86	theme	drug	1157:1160	arg1	efficiency					1170:1179	drug loading efficiency	1157:1179	drug loading efficiency	1157:1179	The proper concentrations for WCS and TA in preparing the encapsulated composite using γ-PGA 0.125 mg ml-1 were 6 mg ml-1 and 1 mg ml-1, respectively; The encapsulation efficiency and drug loading efficiency of WCS-γ-PGA-TA composite were (93.99 ± 2.20)% and (10.73 ± 0.75)%, respectively.					
33444280	2	87	theme	poor	313:316	arg1	solubility					324:333	the poor water solubility	309:333	the poor water solubility	309:333	However, the poor water solubility and low oral bioavailability of TA limited its clinical application.					
33444280	3	88	dep	water	442:446	arg1	WCS-γ-PGA					493:501	WCS-γ-PGA	493:501	WCS-γ-PGA	493:501	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	3	88	dep	water	442:446	arg1	γ					474:474	γ	474:474	γ	474:474	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	3	88	dep	water	442:446	arg1	acid					487:490	glutamic acid	478:490	water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA)	442:502	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	3	88	dep	water	442:446	arg1	chitosan/poly					458:470	soluble chitosan/poly	450:470	soluble chitosan/poly	450:470	In this paper, TA was encapsulated by water - soluble chitosan/poly - γ - glutamic acid (WCS-γ-PGA) to improve its dissolution and oral bioavailability.					
33444280	4	89	theme	in	586:587	arg1	metabolism					594:603	in vivo metabolism	586:603	in vivo metabolism	586:603	The in vitro dissolution and in vivo metabolism of the encapsulated composite in rats were employed to evaluate the efficiency of the improvement.					
33152862	0	0	theme	technological	71:83	arg1	properties					100:109	Nutritional, technological and structural properties	58:109	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.	0:110	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	5	1	theme	soy	786:788	arg1	protein					790:796	soy protein	786:796	soy protein	786:796	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	9	2	theme	added	1315:1319	arg1	polyphenols					1321:1331	added polyphenols	1315:1331	added polyphenols	1315:1331	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	1	3	contain	have	124:127	arg1	Polyphenols					112:122	Polyphenols	112:122	Polyphenols	112:122	Polyphenols have interesting antioxidant properties and could help prevent certain diseases.					
33152862	1	3	contain	have	124:127	arg2	properties					153:162	interesting antioxidant properties	129:162	interesting antioxidant properties	129:162	Polyphenols have interesting antioxidant properties and could help prevent certain diseases.					
33152862	11	4	theme	2853 cm-1	1680:1688	arg1	bands					1699:1703	the 2923 and 2853 cm-1 infrared bands	1667:1703	the 2923 and 2853 cm-1 infrared bands	1667:1703	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	10	5	theme	optimal	1468:1474	arg1	stability					1484:1492	optimal thermal stability	1468:1492	optimal thermal stability	1468:1492	All the EGs showed optimal thermal stability, while colour and texture parameters were significantly influenced by the type of polyphenol extract added.					
33152862	0	6	theme	structural	89:98	arg1	properties					100:109	Nutritional, technological and structural properties	58:109	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.	0:110	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	4	7	used	used	541:544	arg2	reference					551:559	a reference	549:559	a reference	549:559	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	7	used	used	541:544	arg2	ES					496:497	ES	496:497	ES	496:497	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	7	used	used	541:544	arg2	formulation					483:493	One basic formulation	473:493	One basic formulation (ES)	473:498	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	11	8	theme	bands	1699:1703	arg1	frequency					1636:1644	frequency	1636:1644	frequency	1636:1644	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	11	8	theme	bands	1699:1703	arg1	half-bandwidth					1649:1662	half-bandwidth	1649:1662	half-bandwidth	1649:1662	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	5	9	theme	polyphenol	841:850	arg1	extract					852:858	polyphenol extract	841:858	polyphenol extract (G, O or T)	841:870	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	2	10	dep	them	356:359	arg1	polyphenols					361:371	polyphenols	361:371	them polyphenols	356:371	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	9	11	dep	highest	1421:1427	arg1	P < 0.05					1430:1437	P < 0.05	1430:1437	P < 0.05	1430:1437	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	4	12	theme	basic	477:481	arg1	formulation					483:493	One basic formulation	473:493	One basic formulation (ES)	473:498	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	12	theme	basic	477:481	arg1	ES					496:497	ES	496:497	ES	496:497	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	12	theme	basic	477:481	arg1	reference					551:559	a reference	549:559	a reference	549:559	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	6	13	theme	metal	991:995	arg1	container					997:1005	a metal container	989:1005	a metal container	989:1005	Then, the olive oil was gradually added to the mixture and finally, each mixture was placed in a metal container under pressure and chilled for 24 h until they formed an EG.					
33152862	5	14	theme	extract	852:858	arg1	types					832:836	these types	826:836	these types of polyphenol extract (G, O or T)	826:870	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	5	15	dep	extract	852:858	arg1	T					869:869	T	869:869	T	869:869	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	5	15	dep	extract	852:858	arg1	O					864:864	O	864:864	O	864:864	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	5	15	dep	extract	852:858	arg1	G					861:861	G	861:861	G	861:861	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	7	16	theme	metabolites	1122:1132	arg1	concentrations					1095:1108	concentrations	1095:1108	concentrations of phenolic metabolites	1095:1132	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	11	17	from	differences	1617:1627	arg1	frequency					1636:1644	frequency	1636:1644	frequency	1636:1644	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	11	17	from	differences	1617:1627	arg1	half-bandwidth					1649:1662	half-bandwidth	1649:1662	half-bandwidth	1649:1662	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	3	18	theme	gelling	440:446	arg1	agent					448:452	a cold gelling agent	433:452	a cold gelling agent based on alginate	433:470	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	0	19	theme	phenolic	38:45	arg1	compounds					47:55	phenolic compounds	38:55	phenolic compounds	38:55	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	3	20	contain	containing	395:404	arg2	agent					448:452	a cold gelling agent	433:452	a cold gelling agent based on alginate	433:470	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	3	20	contain	containing	395:404	arg1	EGs					391:393	four EGs	386:393	four EGs containing olive oil, soy protein and a cold gelling agent based on alginate	386:470	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	3	20	contain	containing	395:404	arg2	protein					421:427	soy protein	417:427	soy protein	417:427	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	3	20	contain	containing	395:404	arg2	oil					412:414	olive oil	406:414	olive oil	406:414	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	2	21	theme	alternative	273:283	arg1	system					285:290	a promising alternative system	261:290	a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols	261:371	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	4	22	from	seed	646:649	arg1	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	1	23	theme	certain	187:193	arg1	diseases					195:202	certain diseases	187:202	certain diseases	187:202	Polyphenols have interesting antioxidant properties and could help prevent certain diseases.					
33152862	3	24	theme	olive	406:410	arg1	oil					412:414	olive oil	406:414	olive oil	406:414	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	9	25	theme	acid	1359:1362	arg1	monomers					1374:1381	flavanol monomers	1365:1381	flavanol monomers	1365:1381	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	9	25	theme	acid	1359:1362	arg1	contents					1340:1347	contents	1340:1347	contents of gallic acid	1340:1362	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	9	25	theme	acid	1359:1362	arg1	acid					1359:1362	gallic acid	1352:1362	gallic acid	1352:1362	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	9	25	theme	acid	1359:1362	arg1	derivatives					1387:1397	derivatives	1387:1397	derivatives	1387:1397	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	6	26	theme	olive	904:908	arg1	oil					910:912	the olive oil	900:912	the olive oil	900:912	Then, the olive oil was gradually added to the mixture and finally, each mixture was placed in a metal container under pressure and chilled for 24 h until they formed an EG.					
33152862	0	27	theme	Emulsion	0:7	arg1	gels					9:12	Emulsion gels	0:12	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.	0:110	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	3	28	theme	cold	435:438	arg1	agent					448:452	a cold gelling agent	433:452	a cold gelling agent based on alginate	433:470	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	0	29	theme	delivery	17:24	arg1	systems					26:32	delivery systems	17:32	delivery systems	17:32	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	2	30	theme	bioactive	314:322	arg1	compounds					324:332	several bioactive compounds	306:332	several bioactive compounds	306:332	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	4	31	theme	polyphenol	615:624	arg1	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	8	32	dep	highest	1274:1280	arg1	P < 0.05					1283:1290	P < 0.05	1283:1290	P < 0.05	1283:1290	Hydroxytyrosol was identified in all the EGs, but ESO showed the highest (P < 0.05) content.					
33152862	11	33	dep	frequency	1636:1644	arg1	the					1632:1634	the	1632:1634	the	1632:1634	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	2	34	theme	several	306:312	arg1	compounds					324:332	several bioactive compounds	306:332	several bioactive compounds	306:332	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	4	35	theme	solid	609:613	arg1	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	10	36	theme	thermal	1476:1482	arg1	stability					1484:1492	optimal thermal stability	1468:1492	optimal thermal stability	1468:1492	All the EGs showed optimal thermal stability, while colour and texture parameters were significantly influenced by the type of polyphenol extract added.					
33152862	11	37	theme	2923	1671:1674	arg1	bands					1699:1703	the 2923 and 2853 cm-1 infrared bands	1667:1703	the 2923 and 2853 cm-1 infrared bands	1667:1703	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	5	38	theme	corresponding	744:756	arg1	EGs					758:760	The corresponding EGs	740:760	The corresponding EGs	740:760	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	11	39	theme	infrared	1690:1697	arg1	bands					1699:1703	the 2923 and 2853 cm-1 infrared bands	1667:1703	the 2923 and 2853 cm-1 infrared bands	1667:1703	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	11	40	theme	significant	1605:1615	arg1	differences					1617:1627	No significant differences	1602:1627	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands	1602:1703	No significant differences in the frequency or half-bandwidth of the 2923 and 2853 cm-1 infrared bands were observed.					
33152862	10	41	theme	extract	1587:1593	arg1	type					1568:1571	the type	1564:1571	the type of polyphenol extract added	1564:1599	All the EGs showed optimal thermal stability, while colour and texture parameters were significantly influenced by the type of polyphenol extract added.					
33152862	9	42	theme	flavanol	1365:1372	arg1	acid					1359:1362	gallic acid	1352:1362	gallic acid	1352:1362	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	9	42	theme	flavanol	1365:1372	arg1	monomers					1374:1381	flavanol monomers	1365:1381	flavanol monomers	1365:1381	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	4	43	dep	contained	500:508	arg1	while					562:566	while	562:566	while	562:566	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	10	44	theme	polyphenol	1576:1585	arg1	extract					1587:1593	polyphenol extract	1576:1593	polyphenol extract added	1576:1599	All the EGs showed optimal thermal stability, while colour and texture parameters were significantly influenced by the type of polyphenol extract added.					
33152862	3	45	theme	soy	417:419	arg1	protein					421:427	soy protein	417:427	soy protein	417:427	We produced four EGs containing olive oil, soy protein and a cold gelling agent based on alginate.					
33152862	8	46	theme	highest	1274:1280	arg1	content					1293:1299	the highest (P < 0.05) content	1270:1299	the highest (P < 0.05) content	1270:1299	Hydroxytyrosol was identified in all the EGs, but ESO showed the highest (P < 0.05) content.					
33152862	7	47	theme	EGs	1189:1191	arg1	properties					1169:1178	technological and structural properties	1140:1178	technological and structural properties of these EGs	1140:1191	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	7	47	theme	EGs	1189:1191	arg1	composition					1072:1082	The composition	1068:1082	The composition (including concentrations of phenolic metabolites)	1068:1133	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	7	47	theme	EGs	1189:1191	arg1	concentrations					1095:1108	concentrations	1095:1108	concentrations of phenolic metabolites	1095:1132	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	9	48	with	EGs	1306:1308	arg1	polyphenols					1321:1331	added polyphenols	1315:1331	added polyphenols	1315:1331	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	9	49	theme	highest	1421:1427	arg1	content					1440:1446	the highest (P < 0.05) content	1417:1446	the highest (P < 0.05) content	1417:1446	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	4	50	contain	contained	589:597	arg1	three					578:582	three	578:582	three	578:582	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	50	contain	contained	589:597	arg2	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	2	51	theme	promising	263:271	arg1	system					285:290	a promising alternative system	261:290	a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols	261:371	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	1	52	theme	interesting	129:139	arg1	properties					153:162	interesting antioxidant properties	129:162	interesting antioxidant properties	129:162	Polyphenols have interesting antioxidant properties and could help prevent certain diseases.					
33152862	5	53	theme	types	832:836	arg1	water					809:813	water	809:813	water	809:813	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	5	53	theme	types	832:836	arg1	alginate					799:806	alginate	799:806	alginate	799:806	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	5	53	theme	types	832:836	arg1	protein					790:796	soy protein	786:796	soy protein	786:796	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	5	53	theme	types	832:836	arg1	one					819:821	one	819:821	one	819:821	The corresponding EGs were prepared by mixing soy protein, alginate, water and one of these types of polyphenol extract (G, O or T), using a homogenizer.					
33152862	9	54	theme	gallic	1352:1357	arg1	acid					1359:1362	gallic acid	1352:1362	gallic acid	1352:1362	The EGs with added polyphenols showed contents of gallic acid, flavanol monomers and derivatives, with ESG showing the highest (P < 0.05) content.					
33152862	1	55	theme	antioxidant	141:151	arg1	properties					153:162	interesting antioxidant properties	129:162	interesting antioxidant properties	129:162	Polyphenols have interesting antioxidant properties and could help prevent certain diseases.					
33152862	4	56	from	olive	671:675	arg1	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	2	57	contain	have	225:228	arg1	gels					214:217	Emulsion gels	205:217	Emulsion gels (EGs)	205:223	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	2	57	contain	have	225:228	arg1	EGs					220:222	EGs	220:222	EGs	220:222	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	2	57	contain	have	225:228	arg2	characteristics					230:244	characteristics	230:244	characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols	230:371	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	0	58	theme	Nutritional	58:68	arg1	properties					100:109	Nutritional, technological and structural properties	58:109	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.	0:110	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	2	59	theme	Emulsion	205:212	arg1	gels					214:217	Emulsion gels	205:217	Emulsion gels (EGs)	205:223	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	2	59	theme	Emulsion	205:212	arg1	EGs					220:222	EGs	220:222	EGs	220:222	Emulsion gels (EGs) have characteristics that make them a promising alternative system for supplying several bioactive compounds simultaneously, among them polyphenols.					
33152862	0	60	dep	gels	9:12	arg1	properties					100:109	Nutritional, technological and structural properties	58:109	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.	0:110	Emulsion gels as delivery systems for phenolic compounds: Nutritional, technological and structural properties.					
33152862	7	61	theme	phenolic	1113:1120	arg1	metabolites					1122:1132	phenolic metabolites	1113:1132	phenolic metabolites	1113:1132	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	4	62	contain	contained	500:508	arg1	formulation					483:493	One basic formulation	473:493	One basic formulation (ES)	473:498	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	62	contain	contained	500:508	arg1	ES					496:497	ES	496:497	ES	496:497	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	62	contain	contained	500:508	arg2	ingredients					521:531	these ingredients	515:531	these ingredients	515:531	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	4	62	contain	contained	500:508	arg1	reference					551:559	a reference	549:559	a reference	549:559	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	7	63	theme	technological	1140:1152	arg1	properties					1169:1178	technological and structural properties	1140:1178	technological and structural properties of these EGs	1140:1191	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	7	63	theme	technological	1140:1152	arg1	concentrations					1095:1108	concentrations	1095:1108	concentrations of phenolic metabolites	1095:1132	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	4	64	theme	different	599:607	arg1	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
33152862	10	65	theme	texture	1512:1518	arg1	parameters					1520:1529	colour and texture parameters	1501:1529	colour and texture parameters	1501:1529	All the EGs showed optimal thermal stability, while colour and texture parameters were significantly influenced by the type of polyphenol extract added.					
33152862	7	66	theme	structural	1158:1167	arg1	properties					1169:1178	technological and structural properties	1140:1178	technological and structural properties of these EGs	1140:1191	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	7	66	theme	structural	1158:1167	arg1	concentrations					1095:1108	concentrations	1095:1108	concentrations of phenolic metabolites	1095:1132	The composition (including concentrations of phenolic metabolites), and technological and structural properties of these EGs were evaluated.					
33152862	10	67	theme	colour	1501:1506	arg1	parameters					1520:1529	colour and texture parameters	1501:1529	colour and texture parameters	1501:1529	All the EGs showed optimal thermal stability, while colour and texture parameters were significantly influenced by the type of polyphenol extract added.					
33152862	4	68	from	seed	662:665	arg1	extracts					626:633	different solid polyphenol extracts	599:633	different solid polyphenol extracts	599:633	One basic formulation (ES) contained only these ingredients and was used as a reference, while the other three also contained different solid polyphenol extracts from grape seed (G), grape seed and olive (O) or grape total (T), called ESG, ESO and EST, respectively.					
32708592	2	0	theme	smart	528:532	arg1	textiles					534:541	smart textiles	528:541	smart textiles	528:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	0	1	from	Stability	13:21	arg1	Textiles					75:82	Textiles	75:82	Textiles	75:82	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	0	1	from	Stability	13:21	arg1	Films					65:69	Flat Polymer Films	52:69	Flat Polymer Films	52:69	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	1	2	theme	small	163:167	arg1	dimensions					169:178	their small dimensions	157:178	their small dimensions	157:178	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	5	3	theme	textiles	974:981	arg1	surface					945:951	the surface	941:951	the surface of polymer films and textiles made of cellulose, polyester, and nylon	941:1021	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	2	4	with	plastics	438:445	arg1	applications					492:503	broader applications	484:503	broader applications from food packaging to smart textiles	484:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	3	5	from	Variations	544:553	arg1	thickness					571:579	thickness	571:579	thickness	571:579	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	3	5	from	Variations	544:553	arg1	porosity					558:565	porosity	558:565	porosity	558:565	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	4	6	theme	Mechanical	737:746	arg1	stability					748:756	Mechanical stability	737:756	Mechanical stability of nanocellulose coatings in a wet and dry state	737:805	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	4	6	theme	Mechanical	737:746	arg1	characteristics					820:834	critical characteristics	811:834	critical characteristics for many applications	811:856	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	0	7	from	Adhesion	0:7	arg1	Textiles					75:82	Textiles	75:82	Textiles	75:82	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	0	7	from	Adhesion	0:7	arg1	Films					65:69	Flat Polymer Films	52:69	Flat Polymer Films	52:69	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	2	8	theme	many	313:316	arg1	applications					330:341	many interesting applications	313:341	many interesting applications of these materials	313:360	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	5	9	theme	atomic	1041:1046	arg1	microscopy					1054:1063	atomic force microscopy	1041:1063	atomic force microscopy	1041:1063	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	3	10	theme	coatings	598:605	arg1	thickness					571:579	thickness	571:579	thickness	571:579	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	3	10	theme	coatings	598:605	arg1	porosity					558:565	porosity	558:565	porosity	558:565	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	2	11	theme	composition	392:402	arg1	surfaces					418:425	composition and structure surfaces	392:425	composition and structure surfaces	392:425	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	11	theme	composition	392:402	arg1	plastics					438:445	plastics	438:445	plastics	438:445	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	11	theme	composition	392:402	arg1	coatings					456:463	polymer coatings	448:463	polymer coatings	448:463	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	11	theme	composition	392:402	arg1	textiles					470:477	textiles	470:477	textiles with broader applications from food packaging to smart textiles	470:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	4	12	theme	coatings	775:782	arg1	stability					748:756	Mechanical stability	737:756	Mechanical stability of nanocellulose coatings in a wet and dry state	737:805	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	4	12	theme	coatings	775:782	arg1	characteristics					820:834	critical characteristics	811:834	critical characteristics for many applications	811:856	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	6	13	theme	chemical	1178:1185	arg1	cross-linking					1187:1199	physical and chemical cross-linking	1165:1199	physical and chemical cross-linking with added polymers	1165:1219	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	6	14	theme	polycarboxylic	1225:1238	arg1	acids					1240:1244	polycarboxylic acids	1225:1244	polycarboxylic acids	1225:1244	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	2	15	from	packaging	515:523	arg1	applications					492:503	broader applications	484:503	broader applications from food packaging to smart textiles	484:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	6	16	theme	physical	1165:1172	arg1	cross-linking					1187:1199	physical and chemical cross-linking	1165:1199	physical and chemical cross-linking with added polymers	1165:1219	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	4	17	theme	critical	811:818	arg1	stability					748:756	Mechanical stability	737:756	Mechanical stability of nanocellulose coatings in a wet and dry state	737:805	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	4	17	theme	critical	811:818	arg1	characteristics					820:834	critical characteristics	811:834	critical characteristics for many applications	811:856	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	1	18	theme	high	181:184	arg1	area					203:206	high specific surface area	181:206	high specific surface area	181:206	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	2	19	theme	broader	484:490	arg1	applications					492:503	broader applications	484:503	broader applications from food packaging to smart textiles	484:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	20	with	coatings	456:463	arg1	applications					492:503	broader applications	484:503	broader applications from food packaging to smart textiles	484:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	7	21	theme	ability	1337:1343	arg1	effect					1299:1304	the effect	1295:1304	the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion	1295:1428	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	4	22	theme	wet	789:791	arg1	state					801:805	a wet and dry state	787:805	a wet and dry state	787:805	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	7	23	theme	substrate	1313:1321	arg1	structure					1323:1331	the substrate structure	1309:1331	the substrate structure	1309:1331	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	1	24	theme	specific	186:193	arg1	area					203:206	high specific surface area	181:206	high specific surface area	181:206	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	1	25	theme	Renewable	85:93	arg1	materials					109:117	Renewable nanocellulose materials	85:117	Renewable nanocellulose materials	85:117	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	7	26	theme	coating	1413:1419	arg1	adhesion					1421:1428	the coating adhesion	1409:1428	the coating adhesion	1409:1428	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	1	27	theme	surface	195:201	arg1	area					203:206	high specific surface area	181:206	high specific surface area	181:206	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	5	28	theme	nanocrystalline	893:907	arg1	coatings					919:926	nanofibrillated and nanocrystalline cellulose coatings	873:926	nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon	873:1021	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	5	29	theme	force	1048:1052	arg1	microscopy					1054:1063	atomic force microscopy	1041:1063	atomic force microscopy	1041:1063	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	5	30	theme	polymer	956:962	arg1	films					964:968	polymer films	956:968	polymer films	956:968	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	1	31	theme	nanocellulose	95:107	arg1	materials					109:117	Renewable nanocellulose materials	85:117	Renewable nanocellulose materials	85:117	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	3	32	used	used	611:614	arg2	Variations					544:553	Variations	544:553	Variations in porosity and thickness of nanocellulose coatings	544:605	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	6	33	theme	added	1206:1210	arg1	polymers					1212:1219	added polymers	1206:1219	added polymers	1206:1219	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	2	34	theme	interesting	318:328	arg1	applications					330:341	many interesting applications	313:341	many interesting applications of these materials	313:360	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	7	35	theme	particles	1362:1370	arg1	ability					1337:1343	ability	1337:1343	ability of nanocellulose particles to intercalate into the substrate on the coating adhesion	1337:1428	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	7	35	theme	particles	1362:1370	arg1	structure					1323:1331	the substrate structure	1309:1331	the substrate structure	1309:1331	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	0	36	theme	Coatings	40:47	arg1	Stability					13:21	Stability	13:21	Stability	13:21	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	0	36	theme	Coatings	40:47	arg1	Adhesion					0:7	Adhesion	0:7	Adhesion	0:7	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	3	37	theme	particles	661:669	arg1	load					628:631	a load	626:631	a load of functional molecules and particles	626:669	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	1	38	theme	high	209:212	arg1	characteristics					225:239	high mechanical characteristics	209:239	high mechanical characteristics	209:239	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	2	39	theme	polymer	448:454	arg1	coatings					456:463	polymer coatings	448:463	polymer coatings	448:463	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	0	40	theme	Nanocellulose	26:38	arg1	Coatings					40:47	Nanocellulose Coatings	26:47	Nanocellulose Coatings	26:47	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	2	41	theme	food	510:513	arg1	packaging					515:523	food packaging	510:523	food packaging to smart textiles	510:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	3	42	theme	functional	636:645	arg1	molecules					647:655	functional molecules	636:655	functional molecules	636:655	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	2	43	with	textiles	470:477	arg1	applications					492:503	broader applications	484:503	broader applications from food packaging to smart textiles	484:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	1	44	theme	mechanical	214:223	arg1	characteristics					225:239	high mechanical characteristics	209:239	high mechanical characteristics	209:239	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	0	45	theme	Flat	52:55	arg1	Films					65:69	Flat Polymer Films	52:69	Flat Polymer Films	52:69	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	6	46	with	cross-linking	1187:1199	arg1	polymers					1212:1219	added polymers	1206:1219	added polymers	1206:1219	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	4	47	theme	nanocellulose	761:773	arg1	coatings					775:782	nanocellulose coatings	761:782	nanocellulose coatings	761:782	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	5	48	theme	T-peel	1084:1089	arg1	tests					1100:1104	T-peel adhesion tests	1084:1104	T-peel adhesion tests	1084:1104	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	5	49	theme	adhesion	1091:1098	arg1	tests					1100:1104	T-peel adhesion tests	1084:1104	T-peel adhesion tests	1084:1104	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	7	50	theme	structure	1323:1331	arg1	effect					1299:1304	the effect	1295:1304	the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion	1295:1428	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	1	51	theme	increased	128:136	arg1	attention					138:146	increased attention	128:146	increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability	128:277	Renewable nanocellulose materials received increased attention owing to their small dimensions, high specific surface area, high mechanical characteristics, biocompatibility, and compostability.					
32708592	3	52	theme	filtration	714:723	arg1	properties					725:734	filtration properties	714:734	filtration properties	714:734	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	2	53	theme	Nanocellulose	280:292	arg1	coatings					294:301	Nanocellulose coatings	280:301	Nanocellulose coatings	280:301	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	5	54	theme	films	964:968	arg1	surface					945:951	the surface	941:951	the surface of polymer films and textiles made of cellulose, polyester, and nylon	941:1021	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	4	55	theme	dry	797:799	arg1	state					801:805	a wet and dry state	787:805	a wet and dry state	787:805	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	6	56	dep	coatings	1126:1133	arg1	stability					1149:1157	stability	1149:1157	stability	1149:1157	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	6	56	dep	coatings	1126:1133	arg1	adhesion					1136:1143	adhesion	1136:1143	adhesion	1136:1143	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	6	56	dep	coatings	1126:1133	arg1	coatings					1126:1133	coatings' adhesion and stability	1126:1157	coatings' adhesion and stability	1126:1157	Methods to improve coatings' adhesion and stability using physical and chemical cross-linking with added polymers and polycarboxylic acids are analyzed in this study.					
32708592	3	57	theme	nanocellulose	584:596	arg1	coatings					598:605	nanocellulose coatings	584:605	nanocellulose coatings	584:605	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	2	58	theme	materials	352:360	arg1	applications					330:341	many interesting applications	313:341	many interesting applications of these materials	313:360	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	4	59	theme	many	840:843	arg1	applications					845:856	many applications	840:856	many applications	840:856	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
32708592	5	60	theme	cellulose	909:917	arg1	coatings					919:926	nanofibrillated and nanocrystalline cellulose coatings	873:926	nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon	873:1021	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	3	61	theme	molecules	647:655	arg1	load					628:631	a load	626:631	a load of functional molecules and particles	626:669	Variations in porosity and thickness of nanocellulose coatings are used to adjust a load of functional molecules and particles into the coatings, their permeability, and filtration properties.					
32708592	5	62	theme	nanofibrillated	873:887	arg1	coatings					919:926	nanofibrillated and nanocrystalline cellulose coatings	873:926	nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon	873:1021	In this work, nanofibrillated and nanocrystalline cellulose coatings deposited on the surface of polymer films and textiles made of cellulose, polyester, and nylon are studied using atomic force microscopy, ellipsometry, and T-peel adhesion tests.					
32708592	2	63	theme	structure	408:416	arg1	surfaces					418:425	composition and structure surfaces	392:425	composition and structure surfaces	392:425	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	63	theme	structure	408:416	arg1	plastics					438:445	plastics	438:445	plastics	438:445	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	63	theme	structure	408:416	arg1	coatings					456:463	polymer coatings	448:463	polymer coatings	448:463	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	2	63	theme	structure	408:416	arg1	textiles					470:477	textiles	470:477	textiles with broader applications from food packaging to smart textiles	470:541	Nanocellulose coatings are among many interesting applications of these materials to functionalize different by composition and structure surfaces, including plastics, polymer coatings, and textiles with broader applications from food packaging to smart textiles.					
32708592	7	64	theme	nanocellulose	1348:1360	arg1	particles					1362:1370	nanocellulose particles	1348:1370	nanocellulose particles	1348:1370	The paper reports on the effect of the substrate structure and ability of nanocellulose particles to intercalate into the substrate on the coating adhesion.					
32708592	0	65	theme	Polymer	57:63	arg1	Films					65:69	Flat Polymer Films	52:69	Flat Polymer Films	52:69	Adhesion and Stability of Nanocellulose Coatings on Flat Polymer Films and Textiles.					
32708592	4	66	from	stability	748:756	arg1	state					801:805	a wet and dry state	787:805	a wet and dry state	787:805	Mechanical stability of nanocellulose coatings in a wet and dry state are critical characteristics for many applications.					
33515939	0	0	theme	hydrogen	92:99	arg1	peroxide					101:108	hydrogen peroxide	92:108	hydrogen peroxide	92:108	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	2	1	theme	products	498:505	arg1	loss					473:476	the loss	469:476	the loss of these small-size products during recovery	469:521	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	6	2	dep	species	1062:1068	arg1	POS					1058:1060	several POS species	1050:1068	several POS species with different sizes and structures	1050:1104	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	2	3	theme	small-size	487:496	arg1	products					498:505	these small-size products	481:505	these small-size products	481:505	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	3	4	dep	yield	710:714	arg1	POS					706:708	precipitable POS yield	693:714	precipitable POS yield	693:714	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	2	5	theme	higher	373:378	arg1	amounts					380:386	higher amounts	373:386	higher amounts of DP1 and DP2	373:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	5	theme	higher	373:378	arg1	DP2					399:401	DP2	399:401	DP2	399:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	5	theme	higher	373:378	arg1	DP1					391:393	DP1	391:393	DP1	391:393	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	1	6	theme	550 W	257:261	arg1	power					248:252	a microwave power	236:252	a microwave power of 550 W for 10 min	236:272	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	2	7	theme	lower	537:541	arg1	yield					547:551	a lower POS yield	535:551	a lower POS yield (25.0%)	535:559	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	7	theme	lower	537:541	arg1	%					558:558	25.0%	554:558	25.0%	554:558	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	0	8	from	Production	0:9	arg1	pomelo					43:48	pomelo	43:48	pomelo	43:48	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	2	9	theme	alkali-extracted	577:592	arg1	pectin					594:599	the alkali-extracted pectin	573:599	the alkali-extracted pectin (57.7%)	573:607	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	9	theme	alkali-extracted	577:592	arg1	%					606:606	57.7%	602:606	57.7%	602:606	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	4	10	theme	relative	721:728	arg1	amount					730:735	The relative amount	717:735	The relative amount of large-sized POS	717:754	The relative amount of large-sized POS decreased as the H2O2 concentration increased.					
33515939	4	10	theme	relative	721:728	arg1	POS					752:754	large-sized POS	740:754	large-sized POS	740:754	The relative amount of large-sized POS decreased as the H2O2 concentration increased.					
33515939	5	11	theme	average	900:906	arg1	lower					927:931	lower	927:931	lower	927:931	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	5	11	theme	average	900:906	arg1	size					908:911	the average size	896:911	the average size	896:911	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	2	12	theme	acid-extracted	323:336	arg1	pectin					356:361	the acid-extracted methyl-esterified pectin	319:361	the acid-extracted methyl-esterified pectin	319:361	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	1	13	theme	%	204:204	arg1	H2O2					225:228	H2O2	225:228	H2O2	225:228	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	1	13	theme	%	204:204	arg1	peroxide					215:222	1% hydrogen peroxide	203:222	1% hydrogen peroxide (H2O2)	203:229	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	6	14	theme	species	1062:1068	arg1	existence					1037:1045	the existence	1033:1045	the existence of several POS species with different sizes and structures	1033:1104	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	6	15	theme	composition	955:965	arg1	results					938:944	The results	934:944	The results of sugar composition and identification of the degraded products with ESI-MS	934:1021	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	1	16	theme	1	203:203	arg1	%					204:204	%	204:204	%	204:204	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	0	17	from	pomelo	43:48	arg1	pectic-oligosaccharides					14:36	pectic-oligosaccharides	14:36	pectic-oligosaccharides from pomelo	14:48	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	0	17	from	pomelo	43:48	arg1	Production					0:9	Production	0:9	Production of pectic-oligosaccharides from pomelo	0:48	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	2	18	theme	DP2	399:401	arg1	amounts					380:386	higher amounts	373:386	higher amounts of DP1 and DP2	373:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	18	theme	DP2	399:401	arg1	DP2					399:401	DP2	399:401	DP2	399:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	18	theme	DP2	399:401	arg1	DP1					391:393	DP1	391:393	DP1	391:393	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	19	theme	57.7	602:605	arg1	pectin					594:599	the alkali-extracted pectin	573:599	the alkali-extracted pectin (57.7%)	573:607	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	19	theme	57.7	602:605	arg1	%					606:606	57.7%	602:606	57.7%	602:606	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	6	20	theme	degraded	993:1000	arg1	products					1002:1009	the degraded products	989:1009	the degraded products	989:1009	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	3	21	with	Degradation	610:620	arg1	H2O2					667:670	3 and 5% H2O2	658:670	3 and 5% H2O2	658:670	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	1	22	theme	hydrogen	206:213	arg1	H2O2					225:228	H2O2	225:228	H2O2	225:228	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	1	22	theme	hydrogen	206:213	arg1	peroxide					215:222	1% hydrogen peroxide	203:222	1% hydrogen peroxide (H2O2)	203:229	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	3	23	from	decrease	681:688	arg1	yield					710:714	precipitable POS yield	693:714	precipitable POS yield	693:714	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	3	24	dep	%	665:665	arg1	5					664:664	5	664:664	5	664:664	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	3	24	dep	%	665:665	arg1	3					658:658	3	658:658	3	658:658	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	1	25	theme	Oxidative	111:119	arg1	depolymerization					121:136	Oxidative depolymerization	111:136	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins	111:181	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	2	26	theme	ester-free	429:438	arg1	pectin					457:462	the nearly ester-free alkali-extracted pectin	418:462	the nearly ester-free alkali-extracted pectin	418:462	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	0	27	theme	pectic-oligosaccharides	14:36	arg1	Production					0:9	Production	0:9	Production of pectic-oligosaccharides from pomelo	0:48	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	3	28	theme	precipitable	693:704	arg1	yield					710:714	precipitable POS yield	693:714	precipitable POS yield	693:714	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	2	29	dep	resulted	523:530	arg1	compared					561:568	compared	561:568	compared to the alkali-extracted pectin (57.7%)	561:607	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	5	30	theme	significant	855:865	arg1	effect					867:872	no significant effect	852:872	no significant effect	852:872	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	1	31	theme	pomelo	168:173	arg1	pectins					175:181	alkali- and acid-extracted pomelo pectins	141:181	alkali- and acid-extracted pomelo pectins	141:181	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	3	32	theme	alkali-extracted	629:644	arg1	pectin					646:651	the alkali-extracted pectin	625:651	the alkali-extracted pectin	625:651	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	4	33	theme	H2O2	773:776	arg1	concentration					778:790	the H2O2 concentration	769:790	the H2O2 concentration	769:790	The relative amount of large-sized POS decreased as the H2O2 concentration increased.					
33515939	6	34	theme	products	1002:1009	arg1	identification					971:984	identification	971:984	identification	971:984	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	6	34	theme	products	1002:1009	arg1	composition					955:965	sugar composition	949:965	sugar composition	949:965	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	3	35	dep	H2O2	667:670	arg1	%					665:665	%	665:665	%	665:665	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	6	36	theme	sugar	949:953	arg1	composition					955:965	sugar composition	949:965	sugar composition	949:965	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	5	37	from	increase	806:813	arg1	power					832:836	the microwave power	818:836	the microwave power to 1100 W	818:846	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	6	38	theme	identification	971:984	arg1	results					938:944	The results	934:944	The results of sugar composition and identification of the degraded products with ESI-MS	934:1021	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	2	39	dep	yield	547:551	arg1	POS					543:545	a lower POS yield	535:551	a lower POS yield (25.0%)	535:559	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	6	40	theme	several	1050:1056	arg1	species					1062:1068	several POS species	1050:1068	several POS species with different sizes and structures	1050:1104	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	6	41	theme	different	1075:1083	arg1	sizes					1085:1089	different sizes	1075:1089	different sizes	1075:1089	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	2	42	contain	contained	363:371	arg2	DP1					391:393	DP1	391:393	DP1	391:393	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	42	contain	contained	363:371	arg2	amounts					380:386	higher amounts	373:386	higher amounts of DP1 and DP2	373:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	42	contain	contained	363:371	arg1	POS					300:302	POS	300:302	POS	300:302	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	42	contain	contained	363:371	arg1	Pectic-oligosaccharides					275:297	Pectic-oligosaccharides	275:297	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin	275:361	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	42	contain	contained	363:371	arg2	DP2					399:401	DP2	399:401	DP2	399:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	6	43	with	composition	955:965	arg1	ESI-MS					1016:1021	ESI-MS	1016:1021	ESI-MS	1016:1021	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	1	44	theme	alkali-	141:147	arg1	pectins					175:181	alkali- and acid-extracted pomelo pectins	141:181	alkali- and acid-extracted pomelo pectins	141:181	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	6	45	with	identification	971:984	arg1	ESI-MS					1016:1021	ESI-MS	1016:1021	ESI-MS	1016:1021	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	4	46	theme	POS	752:754	arg1	amount					730:735	The relative amount	717:735	The relative amount of large-sized POS	717:754	The relative amount of large-sized POS decreased as the H2O2 concentration increased.					
33515939	4	46	theme	POS	752:754	arg1	POS					752:754	large-sized POS	740:754	large-sized POS	740:754	The relative amount of large-sized POS decreased as the H2O2 concentration increased.					
33515939	2	47	theme	methyl-esterified	338:354	arg1	pectin					356:361	the acid-extracted methyl-esterified pectin	319:361	the acid-extracted methyl-esterified pectin	319:361	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	3	48	theme	pectin	646:651	arg1	Degradation					610:620	Degradation	610:620	Degradation of the alkali-extracted pectin with 3 and 5% H2O2	610:670	Degradation of the alkali-extracted pectin with 3 and 5% H2O2 led to a decrease in precipitable POS yield.					
33515939	4	49	theme	large-sized	740:750	arg1	POS					752:754	large-sized POS	740:754	large-sized POS	740:754	The relative amount of large-sized POS decreased as the H2O2 concentration increased.					
33515939	1	50	theme	acid-extracted	153:166	arg1	pectins					175:181	alkali- and acid-extracted pomelo pectins	141:181	alkali- and acid-extracted pomelo pectins	141:181	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	5	51	theme	overall	877:883	arg1	yield					885:889	overall yield	877:889	overall yield	877:889	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	0	52	with	pectin	55:60	arg1	peroxide					101:108	hydrogen peroxide	92:108	hydrogen peroxide	92:108	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	2	53	theme	DP1	391:393	arg1	amounts					380:386	higher amounts	373:386	higher amounts of DP1 and DP2	373:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	53	theme	DP1	391:393	arg1	DP2					399:401	DP2	399:401	DP2	399:401	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	2	53	theme	DP1	391:393	arg1	DP1					391:393	DP1	391:393	DP1	391:393	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	5	54	theme	microwave	822:830	arg1	power					832:836	the microwave power	818:836	the microwave power to 1100 W	818:846	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	1	55	theme	microwave	238:246	arg1	power					248:252	a microwave power	236:252	a microwave power of 550 W for 10 min	236:272	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33515939	0	56	theme	oxidative	65:73	arg1	degradation					75:85	oxidative degradation	65:85	oxidative degradation	65:85	Production of pectic-oligosaccharides from pomelo peel pectin by oxidative degradation with hydrogen peroxide.					
33515939	6	57	with	species	1062:1068	arg1	structures					1095:1104	structures	1095:1104	structures	1095:1104	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	6	57	with	species	1062:1068	arg1	sizes					1085:1089	different sizes	1075:1089	different sizes	1075:1089	The results of sugar composition and identification of the degraded products with ESI-MS confirmed the existence of several POS species with different sizes and structures.					
33515939	2	58	theme	alkali-extracted	440:455	arg1	pectin					457:462	the nearly ester-free alkali-extracted pectin	418:462	the nearly ester-free alkali-extracted pectin	418:462	Pectic-oligosaccharides (POS) produced from the acid-extracted methyl-esterified pectin contained higher amounts of DP1 and DP2 than that from the nearly ester-free alkali-extracted pectin, and the loss of these small-size products during recovery resulted in a lower POS yield (25.0%) compared to the alkali-extracted pectin (57.7%).					
33515939	5	59	contain	had	848:850	arg1	increase					806:813	An increase	803:813	An increase in the microwave power to 1100 W	803:846	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	5	59	contain	had	848:850	arg2	effect					867:872	no significant effect	852:872	no significant effect	852:872	An increase in the microwave power to 1100 W had no significant effect on overall yield, but the average size shifted to be lower.					
33515939	1	60	theme	pectins	175:181	arg1	depolymerization					121:136	Oxidative depolymerization	111:136	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins	111:181	Oxidative depolymerization of alkali- and acid-extracted pomelo pectins was performed using 1% hydrogen peroxide (H2O2) with a microwave power of 550 W for 10 min.					
33436937	4	0	theme	biomass	930:936	arg1	entrapped					938:946	biomass entrapped	930:946	biomass entrapped	930:946	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	1	1	theme	mainstream	329:338	arg1	treatment					316:324	the treatment	312:324	the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	312:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	3	2	theme	RGO	712:714	arg1	addition					716:723	The RGO addition	708:723	The RGO addition to the SA matrix	708:740	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	1	3	theme	growth	146:151	arg1	limitations					260:270	significant limitations	248:270	significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	248:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	3	theme	growth	146:151	arg1	temperatures					175:186	high optimal temperatures	162:186	high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria	162:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	3	theme	growth	146:151	arg1	rate					153:156	The slow growth rate	137:156	The slow growth rate	137:156	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	3	4	theme	beads	843:847	arg1	properties					821:830	the mechanical properties	806:830	the mechanical properties of the gel beads	806:847	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	0	5	theme	microbial	67:75	arg1	community					77:85	the microbial community	63:85	the microbial community	63:85	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	4	6	dep	SA	951:952	arg1	beads					969:973	gel beads	965:973	gel beads	965:973	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	5	7	theme	new	1289:1291	arg1	conditions					1301:1310	the new process conditions	1285:1310	the new process conditions	1285:1310	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	5	8	theme	gene	1219:1222	arg1	abundance					1224:1232	relative gene abundance	1210:1232	relative gene abundance	1210:1232	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	4	9	theme	nitrogen	892:899	arg1	rate					909:912	the nitrogen removal rate	888:912	the nitrogen removal rate	888:912	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	1	10	theme	wastewater	343:352	arg1	WWTP					391:394	wastewater entering wastewater treatment plant (WWTP)	343:395	wastewater entering wastewater treatment plant (WWTP)	343:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	11	from	application	297:307	arg1	treatment					316:324	the treatment	312:324	the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	312:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	6	12	theme	nitrogen	1453:1460	arg1	transformation					1462:1475	the nitrogen transformation	1449:1475	the nitrogen transformation inside the beads	1449:1492	The microbial community inside the gel beads was completely remodelled, in comparison with inoculum, and denitrification contributed to the nitrogen transformation inside the beads.					
33436937	4	13	theme	non-immobilized	992:1006	arg1	biomass					1008:1014	non-immobilized biomass	992:1014	non-immobilized biomass	992:1014	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	1	14	theme	significant	248:258	arg1	rate					153:156	The slow growth rate	137:156	The slow growth rate	137:156	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	14	theme	significant	248:258	arg1	temperatures					175:186	high optimal temperatures	162:186	high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria	162:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	14	theme	significant	248:258	arg1	limitations					260:270	significant limitations	248:270	significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	248:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	15	theme	entering	354:361	arg1	WWTP					391:394	wastewater entering wastewater treatment plant (WWTP)	343:395	wastewater entering wastewater treatment plant (WWTP)	343:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	4	16	theme	gel	965:967	arg1	beads					969:973	gel beads	965:973	gel beads	965:973	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	2	17	theme	particular	612:621	arg1	emphasis					623:630	a particular emphasis	610:630	a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP	610:705	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	5	18	theme	process	1293:1299	arg1	conditions					1301:1310	the new process conditions	1285:1310	the new process conditions	1285:1310	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	1	19	theme	wastewater	363:372	arg1	WWTP					391:394	wastewater entering wastewater treatment plant (WWTP)	343:395	wastewater entering wastewater treatment plant (WWTP)	343:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	6	20	with	comparison	1388:1397	arg1	inoculum					1404:1411	inoculum	1404:1411	inoculum	1404:1411	The microbial community inside the gel beads was completely remodelled, in comparison with inoculum, and denitrification contributed to the nitrogen transformation inside the beads.					
33436937	3	21	theme	swelling	774:781	arg1	suppression					749:759	suppression	749:759	suppression	749:759	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	0	22	theme	nitrogen	99:106	arg1	removal					108:114	and nitrogen removal	95:114	removal	108:114	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	1	23	theme	treatment	374:382	arg1	WWTP					391:394	wastewater entering wastewater treatment plant (WWTP)	343:395	wastewater entering wastewater treatment plant (WWTP)	343:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	24	theme	community	463:471	arg1	changes					473:479	microbial community changes	453:479	microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	453:564	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	4	25	theme	removal	901:907	arg1	rate					909:912	the nitrogen removal rate	888:912	the nitrogen removal rate	888:912	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	1	26	dep	processes	287:295	arg1	application					297:307	application	297:307	application	297:307	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	27	theme	high	162:165	arg1	temperatures					175:186	high optimal temperatures	162:186	high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria	162:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	27	theme	high	162:165	arg1	rate					153:156	The slow growth rate	137:156	The slow growth rate	137:156	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	27	theme	high	162:165	arg1	limitations					260:270	significant limitations	248:270	significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	248:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	28	theme	nitrogen	432:439	arg1	removal					441:447	the nitrogen removal	428:447	the nitrogen removal	428:447	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	1	29	theme	plant	384:388	arg1	WWTP					391:394	wastewater entering wastewater treatment plant (WWTP)	343:395	wastewater entering wastewater treatment plant (WWTP)	343:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	6	30	theme	gel	1348:1350	arg1	beads					1352:1356	the gel beads	1344:1356	the gel beads	1344:1356	The microbial community inside the gel beads was completely remodelled, in comparison with inoculum, and denitrification contributed to the nitrogen transformation inside the beads.					
33436937	2	31	from	removal	441:447	arg1	carriers					557:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	1	32	theme	optimal	167:173	arg1	temperatures					175:186	high optimal temperatures	162:186	high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria	162:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	32	theme	optimal	167:173	arg1	rate					153:156	The slow growth rate	137:156	The slow growth rate	137:156	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	32	theme	optimal	167:173	arg1	limitations					260:270	significant limitations	248:270	significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	248:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	33	theme	process	584:590	arg1	temperature					592:602	the process temperature	580:602	the process temperature	580:602	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	0	34	theme	graphene	45:52	arg1	oxide					54:58	reduced graphene oxide	37:58	reduced graphene oxide	37:58	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	2	35	from	changes	473:479	arg1	carriers					557:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	0	36	theme	immobilization	18:31	arg1	Effect					0:5	Effect	0:5	Effect of biomass immobilization and reduced graphene oxide on the microbial community	0:85	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	5	37	theme	next-generation	1114:1128	arg1	NGS					1142:1144	NGS	1142:1144	NGS	1142:1144	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	5	37	theme	next-generation	1114:1128	arg1	sequencing					1130:1139	next-generation sequencing	1114:1139	next-generation sequencing (NGS)	1114:1145	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	0	38	theme	low	119:121	arg1	temperatures					123:134	low temperatures	119:134	low temperatures	119:134	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	4	39	theme	"	1045:1045	arg1	effect					1047:1052	a ''protective" effect	1031:1052	a ''protective" effect caused by immobilization	1031:1077	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	0	40	theme	biomass	10:16	arg1	immobilization					18:31	biomass immobilization	10:31	biomass immobilization	10:31	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	1	41	theme	anammox	279:285	arg1	processes					287:295	the anammox processes	275:295	the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	275:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	42	theme	graphene	533:540	arg1	SA-RGO					549:554	SA-RGO	549:554	SA-RGO	549:554	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	2	42	theme	graphene	533:540	arg1	oxide					542:546	sodium alginate-reduced graphene oxide	509:546	sodium alginate-reduced graphene oxide (SA-RGO)	509:555	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	4	43	theme	drop	880:883	arg1	effect					854:859	The effect	850:859	The effect of the temperature drop on the nitrogen removal rate	850:912	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	5	44	theme	relative	1210:1217	arg1	abundance					1224:1232	relative gene abundance	1210:1232	relative gene abundance	1210:1232	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	4	45	theme	protective	-1:8	arg1	effect					1047:1052	a ''protective" effect	1031:1052	a ''protective" effect caused by immobilization	1031:1077	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	0	46	theme	reduced	37:43	arg1	oxide					54:58	reduced graphene oxide	37:58	reduced graphene oxide	37:58	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	1	47	theme	processes	287:295	arg1	rate					153:156	The slow growth rate	137:156	The slow growth rate	137:156	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	47	theme	processes	287:295	arg1	temperatures					175:186	high optimal temperatures	162:186	high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria	162:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	47	theme	processes	287:295	arg1	limitations					260:270	significant limitations	248:270	significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	248:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	48	theme	alginate-reduced	516:531	arg1	SA-RGO					549:554	SA-RGO	549:554	SA-RGO	549:554	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	2	48	theme	alginate-reduced	516:531	arg1	oxide					542:546	sodium alginate-reduced graphene oxide	509:546	sodium alginate-reduced graphene oxide (SA-RGO)	509:555	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	1	49	theme	WWTP	391:394	arg1	mainstream					329:338	mainstream	329:338	mainstream of wastewater entering wastewater treatment plant (WWTP)	329:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	4	50	theme	"	1045:1045	arg1	effect					1047:1052	a ''protective" effect	1031:1052	a ''protective" effect caused by immobilization	1031:1077	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	1	51	theme	anaerobic	196:204	arg1	bacteria					235:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	52	theme	microbial	453:461	arg1	changes					473:479	microbial community changes	453:479	microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	453:564	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	2	53	theme	sodium	509:514	arg1	SA-RGO					549:554	SA-RGO	549:554	SA-RGO	549:554	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	2	53	theme	sodium	509:514	arg1	oxide					542:546	sodium alginate-reduced graphene oxide	509:546	sodium alginate-reduced graphene oxide (SA-RGO)	509:555	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	2	54	theme	wastewater	678:687	arg1	mainstream					664:673	the mainstream	660:673	the mainstream of wastewater entering the WWTP	660:705	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	1	55	theme	ammonium	206:213	arg1	bacteria					235:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	0	56	theme	oxide	54:58	arg1	Effect					0:5	Effect	0:5	Effect of biomass immobilization and reduced graphene oxide on the microbial community	0:85	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	6	57	theme	microbial	1317:1325	arg1	community					1327:1335	The microbial community	1313:1335	The microbial community inside the gel beads	1313:1356	The microbial community inside the gel beads was completely remodelled, in comparison with inoculum, and denitrification contributed to the nitrogen transformation inside the beads.					
33436937	0	58	from	Effect	0:5	arg1	community					77:85	the microbial community	63:85	the microbial community	63:85	Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures.					
33436937	2	59	theme	oxide	542:546	arg1	carriers					557:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	3	60	theme	mechanical	810:819	arg1	properties					821:830	the mechanical properties	806:830	the mechanical properties of the gel beads	806:847	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	3	61	theme	SA	732:733	arg1	matrix					735:740	the SA matrix	728:740	the SA matrix	728:740	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	4	62	from	effect	854:859	arg1	rate					909:912	the nitrogen removal rate	888:912	the nitrogen removal rate	888:912	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	1	63	theme	oxidation	215:223	arg1	bacteria					235:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	5	64	theme	conditions	1301:1310	arg1	implementation					1267:1280	the implementation	1263:1280	the implementation of the new process conditions	1263:1310	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	2	65	from	emphasis	623:630	arg1	temperature					639:649	the temperature	635:649	the temperature close to the mainstream of wastewater entering the WWTP	635:705	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	3	66	theme	beads	768:772	arg1	swelling					774:781	the beads swelling	764:781	the beads swelling	764:781	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	2	67	theme	alginate	491:498	arg1	carriers					557:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers	484:564	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	4	68	theme	temperature	868:878	arg1	drop					880:883	the temperature drop	864:883	the temperature drop	864:883	The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ''protective" effect caused by immobilization.					
33436937	5	69	theme	community	1184:1192	arg1	composition					1194:1204	the microbial community composition	1170:1204	the microbial community composition	1170:1204	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
33436937	1	70	theme	anammox	226:232	arg1	bacteria					235:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	the anaerobic ammonium oxidation (anammox) bacteria	192:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	2	71	theme	sodium	484:489	arg1	SA					501:502	SA	501:502	SA	501:502	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	2	71	theme	sodium	484:489	arg1	alginate					491:498	sodium alginate	484:498	sodium alginate (SA)	484:503	In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate-reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP.					
33436937	1	72	theme	slow	141:144	arg1	limitations					260:270	significant limitations	248:270	significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP)	248:395	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	72	theme	slow	141:144	arg1	temperatures					175:186	high optimal temperatures	162:186	high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria	162:242	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	1	72	theme	slow	141:144	arg1	rate					153:156	The slow growth rate	137:156	The slow growth rate	137:156	The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP).					
33436937	3	73	theme	gel	839:841	arg1	beads					843:847	the gel beads	835:847	the gel beads	835:847	The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads.					
33436937	5	74	theme	microbial	1174:1182	arg1	composition					1194:1204	the microbial community composition	1170:1204	the microbial community composition	1170:1204	However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions.					
32691453	2	0	from	wheat	349:353	arg1	control					369:375	the control	365:375	the control	365:375	Wet gluten was added to the PCF and CWF for production of bread, while bread from wheat served as the control.					
32691453	2	0	from	wheat	349:353	arg1	bread					338:342	bread	338:342	bread from wheat	338:353	Wet gluten was added to the PCF and CWF for production of bread, while bread from wheat served as the control.					
32691453	7	1	theme	crude	1027:1031	arg1	calorie					1018:1024	significantly (P < 0.05) higher calorie	986:1024	significantly (P < 0.05) higher calorie	986:1024	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	1	theme	crude	1027:1031	arg1	protein					1033:1039	crude protein	1027:1039	crude protein	1027:1039	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	10	2	theme	comparable	1467:1476	arg1	attributes					1456:1465	some quality attributes	1443:1465	some quality attributes comparable to that of wheat bread	1443:1499	The bread produced had some quality attributes comparable to that of wheat bread.					
32691453	9	3	theme	gluten	1404:1409	arg1	extract					1411:1417	added gluten extract	1398:1417	added gluten extract	1398:1417	The study revealed the feasibility of bread baking from preheated cassava flour with added gluten extract.					
32691453	8	4	from	PCF	1176:1178	arg1	bread					1165:1169	bread	1165:1169	bread from PCF	1165:1178	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	1	5	theme	wheat	248:252	arg1	flour					254:258	wheat flour	248:258	wheat flour	248:258	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	11	6	from	bread	1728:1732	arg1	texture					1750:1756	texture	1750:1756	texture	1750:1756	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	11	6	from	bread	1728:1732	arg1	color					1743:1747	crust color	1737:1747	crust color	1737:1747	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	11	6	from	bread	1728:1732	arg1	acceptability					1771:1783	overall acceptability	1763:1783	overall acceptability	1763:1783	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	6	7	dep	appeared	945:952	arg1	developed					959:967	developed	959:967	appeared less developed	945:967	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	6	8	from	WF	852:853	arg1	Breads					840:845	Breads	840:845	Breads from WF and CWF	840:861	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	0	9	theme	Quality	0:6	arg1	attributes					8:17	Quality attributes	0:17	Quality attributes of breads from high-quality cassava flour	0:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	1	10	theme	breads	161:166	arg1	attributes					147:156	the physical, chemical, and sensory attributes	111:156	the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF)	111:264	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	11	11	from	flour	1560:1564	arg1	Bread					1525:1529	Bread	1525:1529	Bread from wheat-cassava composite flour with added gluten	1525:1582	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	4	12	theme	comparable	577:586	arg1	1.12 mL/g					566:574	1.12 mL/g	566:574	1.12 mL/g	566:574	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	4	12	theme	comparable	577:586	arg1	capacity					556:563	water absorption capacity	539:563	water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g)	539:626	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	6	13	from	CWF	859:861	arg1	Breads					840:845	Breads	840:845	Breads from WF and CWF	840:861	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	5	14	dep	significant	640:650	arg1	P					653:653	P < 0.05	653:660	P < 0.05	653:660	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	4	15	theme	water	539:543	arg1	1.12 mL/g					566:574	1.12 mL/g	566:574	1.12 mL/g	566:574	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	4	15	theme	water	539:543	arg1	capacity					556:563	water absorption capacity	539:563	water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g)	539:626	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	11	16	theme	wheat-cassava	1536:1548	arg1	flour					1560:1564	wheat-cassava composite flour	1536:1564	wheat-cassava composite flour with added gluten	1536:1582	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	4	17	dep	14.21	513:517	arg1	to					510:511	to	510:511	to	510:511	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	11	18	theme	overall	1763:1769	arg1	acceptability					1771:1783	overall acceptability	1763:1783	overall acceptability	1763:1783	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	1	19	theme	high-quality	192:203	arg1	PCF					220:222	PCF	220:222	PCF	220:222	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	1	19	theme	high-quality	192:203	arg1	flour					213:217	preheated high-quality cassava flour	182:217	preheated high-quality cassava flour (PCF)	182:223	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	8	20	from	texture	1250:1256	arg1	different					1202:1210	different	1202:1210	different	1202:1210	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	8	20	from	texture	1250:1256	arg1	color					1243:1247	crust color	1237:1247	crust color	1237:1247	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	5	21	theme	*	712:712	arg1	values					714:719	L* values	711:719	L* values	711:719	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	8	22	theme	%	1220:1220	arg1	bread					1228:1232	100% wheat bread	1217:1232	100% wheat bread in crust color	1217:1247	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	12	23	theme	wheat	1818:1822	arg1	component					1851:1859	a viable component	1842:1859	a viable component to be used in nonwheat flours for bread making	1842:1906	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	12	23	theme	wheat	1818:1822	arg1	extract					1831:1837	wheat gluten extract	1818:1837	wheat gluten extract	1818:1837	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	11	24	theme	added	1571:1575	arg1	gluten					1577:1582	added gluten	1571:1582	added gluten	1571:1582	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	11	25	dep	APPLICATION	1512:1522	arg1	similar					1588:1594	similar	1588:1594	similar	1588:1594	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	1	26	theme	physical	115:122	arg1	attributes					147:156	the physical, chemical, and sensory attributes	111:156	the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF)	111:264	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	7	27	theme	bread	1119:1123	arg1	samples					1125:1131	other bread samples	1113:1131	other bread samples	1113:1131	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	11	28	theme	crust	1737:1741	arg1	color					1743:1747	crust color	1737:1747	crust color	1737:1747	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	12	29	from	study	1804:1808	arg1	Findings					1786:1793	Findings	1786:1793	Findings from the study	1786:1808	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	5	30	theme	specific	773:780	arg1	volume					782:787	specific volume	773:787	specific volume (3.85 to 4.21 mL/g)	773:807	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	8	31	theme	crust	1237:1241	arg1	color					1243:1247	crust color	1237:1247	crust color	1237:1247	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	4	32	theme	wheat	599:603	arg1	WF					612:613	WF	612:613	WF	612:613	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	4	32	theme	wheat	599:603	arg1	1.10 mL/g					617:625	1.10 mL/g	617:625	1.10 mL/g	617:625	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	4	32	theme	wheat	599:603	arg1	flour					605:609	wheat flour	599:609	wheat flour (WF) (1.10 mL/g)	599:626	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	0	33	theme	wet	75:77	arg1	gluten					79:84	wet gluten	75:84	wet gluten	75:84	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	4	34	theme	moisture	444:451	arg1	contents					453:460	The moisture contents	440:460	The moisture contents of the flour samples	440:481	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	11	35	from	volume	1623:1628	arg1	similar					1588:1594	similar	1588:1594	similar	1588:1594	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	1	36	theme	chemical	125:132	arg1	attributes					147:156	the physical, chemical, and sensory attributes	111:156	the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF)	111:264	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	8	37	theme	overall	1263:1269	arg1	acceptability					1271:1283	overall acceptability	1263:1283	overall acceptability	1263:1283	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	11	38	from	firmness	1634:1641	arg1	similar					1588:1594	similar	1588:1594	similar	1588:1594	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	8	39	from	bread	1228:1232	arg1	different					1202:1210	different	1202:1210	different	1202:1210	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	8	39	from	bread	1228:1232	arg1	color					1243:1247	crust color	1237:1247	crust color	1237:1247	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	6	40	theme	crumb	881:885	arg1	microstructure					887:900	similar crumb microstructure	873:900	similar crumb microstructure	873:900	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	8	41	theme	Sensory	1134:1140	arg1	evaluation					1142:1151	Sensory evaluation	1134:1151	Sensory evaluation	1134:1151	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	2	42	theme	bread	325:329	arg1	production					311:320	production	311:320	production of bread	311:329	Wet gluten was added to the PCF and CWF for production of bread, while bread from wheat served as the control.					
32691453	5	43	theme	color	678:682	arg1	characteristics					684:698	color characteristics	678:698	color characteristics	678:698	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	11	44	with	flour	1560:1564	arg1	gluten					1577:1582	added gluten	1571:1582	added gluten	1571:1582	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	2	45	dep	PCF	295:297	arg1	the					291:293	the	291:293	the	291:293	Wet gluten was added to the PCF and CWF for production of bread, while bread from wheat served as the control.					
32691453	6	46	from	PCF	941:943	arg1	bubbles					914:920	gas bubbles	910:920	gas bubbles in the sample from PCF	910:943	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	6	46	from	PCF	941:943	arg1	sample					929:934	the sample	925:934	the sample from PCF	925:943	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	11	47	theme	added	1680:1684	arg1	gluten					1686:1691	added gluten	1680:1691	added gluten	1680:1691	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	7	48	theme	Wheat	970:974	arg1	bread					976:980	Wheat bread	970:980	Wheat bread	970:980	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	1	49	with	flour	213:217	arg1	flour					254:258	wheat flour	248:258	wheat flour	248:258	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	13	50	theme	starch	2007:2012	arg1	industry					2014:2021	the wheat starch industry	1997:2021	the wheat starch industry	1997:2021	This could be a basis to further add value to the gluten churned out as a by-product in the wheat starch industry.					
32691453	3	51	theme	Flour	378:382	arg1	functionality					384:396	Flour functionality	378:396	Flour functionality	378:396	Flour functionality was determined prior to bread production.					
32691453	7	52	dep	significantly	986:998	arg1	P					1001:1001	P < 0.05	1001:1008	P < 0.05	1001:1008	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	3	53	theme	bread	422:426	arg1	production					428:437	bread production	422:437	bread production	422:437	Flour functionality was determined prior to bread production.					
32691453	0	54	theme	cassava	47:53	arg1	flour					55:59	high-quality cassava flour	34:59	high-quality cassava flour	34:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	7	55	theme	lower	1060:1064	arg1	fiber					1072:1076	lower crude fiber	1060:1076	lower crude fiber	1060:1076	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	4	56	theme	flour	469:473	arg1	samples					475:481	the flour samples	465:481	the flour samples	465:481	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	13	57	from	by-product	1983:1992	arg1	industry					2014:2021	the wheat starch industry	1997:2021	the wheat starch industry	1997:2021	This could be a basis to further add value to the gluten churned out as a by-product in the wheat starch industry.					
32691453	10	58	theme	wheat	1489:1493	arg1	bread					1495:1499	wheat bread	1489:1499	wheat bread	1489:1499	The bread produced had some quality attributes comparable to that of wheat bread.					
32691453	9	59	theme	preheated	1369:1377	arg1	flour					1387:1391	preheated cassava flour	1369:1391	preheated cassava flour with added gluten extract	1369:1417	The study revealed the feasibility of bread baking from preheated cassava flour with added gluten extract.					
32691453	4	60	theme	%	518:518	arg1	range					495:499	the range	491:499	the range of 12.80 to 14.21%	491:518	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	7	61	theme	crude	1045:1049	arg1	calorie					1018:1024	significantly (P < 0.05) higher calorie	986:1024	significantly (P < 0.05) higher calorie	986:1024	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	61	theme	crude	1045:1049	arg1	fat					1051:1053	crude fat	1045:1053	crude fat	1045:1053	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	11	62	theme	specific	1614:1621	arg1	volume					1623:1628	specific volume	1614:1628	specific volume	1614:1628	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	5	63	from	firmness	813:820	arg1	similar					762:768	similar	762:768	similar	762:768	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	9	64	theme	added	1398:1402	arg1	extract					1411:1417	added gluten extract	1398:1417	added gluten extract	1398:1417	The study revealed the feasibility of bread baking from preheated cassava flour with added gluten extract.					
32691453	11	65	from	similar	1588:1594	arg1	volume					1623:1628	specific volume	1614:1628	specific volume	1614:1628	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	11	65	from	similar	1588:1594	arg1	firmness					1634:1641	firmness	1634:1641	firmness	1634:1641	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	5	66	dep	volume	782:787	arg1	3.85					790:793	3.85	790:793	3.85	790:793	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	7	67	theme	higher	1011:1016	arg1	calorie					1018:1024	significantly (P < 0.05) higher calorie	986:1024	significantly (P < 0.05) higher calorie	986:1024	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	67	theme	higher	1011:1016	arg1	protein					1033:1039	crude protein	1027:1039	crude protein	1027:1039	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	67	theme	higher	1011:1016	arg1	fat					1051:1053	crude fat	1045:1053	crude fat	1045:1053	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	68	contain	had	982:984	arg2	calorie					1018:1024	significantly (P < 0.05) higher calorie	986:1024	significantly (P < 0.05) higher calorie	986:1024	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	68	contain	had	982:984	arg1	bread					976:980	Wheat bread	970:980	Wheat bread	970:980	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	68	contain	had	982:984	arg2	fat					1051:1053	crude fat	1045:1053	crude fat	1045:1053	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	7	68	contain	had	982:984	arg2	protein					1033:1039	crude protein	1027:1039	crude protein	1027:1039	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	11	69	with	flour	1669:1673	arg1	gluten					1686:1691	added gluten	1680:1691	added gluten	1680:1691	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	2	70	theme	Wet	267:269	arg1	gluten					271:276	Wet gluten	267:276	Wet gluten	267:276	Wet gluten was added to the PCF and CWF for production of bread, while bread from wheat served as the control.					
32691453	1	71	theme	preheated	182:190	arg1	PCF					220:222	PCF	220:222	PCF	220:222	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	1	71	theme	preheated	182:190	arg1	flour					213:217	preheated high-quality cassava flour	182:217	preheated high-quality cassava flour (PCF)	182:223	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	8	72	theme	100	1217:1219	arg1	%					1220:1220	%	1220:1220	%	1220:1220	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	5	73	dep	2.64	831:834	arg1	to					828:829	to	828:829	to	828:829	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	11	74	theme	composite	1550:1558	arg1	flour					1560:1564	wheat-cassava composite flour	1536:1564	wheat-cassava composite flour with added gluten	1536:1582	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	5	75	theme	L	711:711	arg1	values					714:719	L* values	711:719	L* values	711:719	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	8	76	theme	wheat	1222:1226	arg1	bread					1228:1232	100% wheat bread	1217:1232	100% wheat bread in crust color	1217:1247	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	11	77	from	flour	1669:1673	arg1	sample					1649:1654	sample	1649:1654	sample from cassava flour with added gluten	1649:1691	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	4	78	theme	absorption	545:554	arg1	1.12 mL/g					566:574	1.12 mL/g	566:574	1.12 mL/g	566:574	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	4	78	theme	absorption	545:554	arg1	capacity					556:563	water absorption capacity	539:563	water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g)	539:626	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	10	79	contain	had	1439:1441	arg2	attributes					1456:1465	some quality attributes	1443:1465	some quality attributes comparable to that of wheat bread	1443:1499	The bread produced had some quality attributes comparable to that of wheat bread.					
32691453	10	79	contain	had	1439:1441	arg1	bread					1424:1428	The bread	1420:1428	The bread produced	1420:1437	The bread produced had some quality attributes comparable to that of wheat bread.					
32691453	12	80	theme	gluten	1824:1829	arg1	component					1851:1859	a viable component	1842:1859	a viable component to be used in nonwheat flours for bread making	1842:1906	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	12	80	theme	gluten	1824:1829	arg1	extract					1831:1837	wheat gluten extract	1818:1837	wheat gluten extract	1818:1837	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	11	81	theme	PRACTICAL	1502:1510	arg1	APPLICATION					1512:1522	PRACTICAL APPLICATION	1502:1522	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.	1502:1784	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	11	82	theme	wheat	1722:1726	arg1	bread					1728:1732	wheat bread	1722:1732	wheat bread in crust color, texture, and overall acceptability	1722:1783	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	7	83	theme	other	1113:1117	arg1	samples					1125:1131	other bread samples	1113:1131	other bread samples	1113:1131	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	5	84	theme	significant	640:650	arg1	differences					663:673	significant (P < 0.05) differences	640:673	significant (P < 0.05) differences in color characteristics	640:698	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	5	85	from	differences	663:673	arg1	characteristics					684:698	color characteristics	678:698	color characteristics	678:698	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	1	86	theme	sensory	139:145	arg1	attributes					147:156	the physical, chemical, and sensory attributes	111:156	the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF)	111:264	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	0	87	from	attributes	8:17	arg1	flour					55:59	high-quality cassava flour	34:59	high-quality cassava flour	34:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	0	88	from	flour	55:59	arg1	breads					22:27	breads	22:27	breads from high-quality cassava flour	22:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	0	88	from	flour	55:59	arg1	attributes					8:17	Quality attributes	0:17	Quality attributes of breads from high-quality cassava flour	0:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	11	89	theme	wheat	1599:1603	arg1	bread					1605:1609	wheat bread	1599:1609	wheat bread	1599:1609	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	13	90	theme	wheat	2001:2005	arg1	industry					2014:2021	the wheat starch industry	1997:2021	the wheat starch industry	1997:2021	This could be a basis to further add value to the gluten churned out as a by-product in the wheat starch industry.					
32691453	11	91	theme	cassava	1661:1667	arg1	flour					1669:1673	cassava flour	1661:1673	cassava flour with added gluten	1661:1691	PRACTICAL APPLICATION: Bread from wheat-cassava composite flour with added gluten was similar to wheat bread in specific volume and firmness while sample from cassava flour with added gluten compared favorably well with wheat bread in crust color, texture, and overall acceptability.					
32691453	6	92	theme	similar	873:879	arg1	microstructure					887:900	similar crumb microstructure	873:900	similar crumb microstructure	873:900	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	0	93	theme	breads	22:27	arg1	attributes					8:17	Quality attributes	0:17	Quality attributes of breads from high-quality cassava flour	0:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	5	94	from	similar	762:768	arg1	volume					782:787	specific volume	773:787	specific volume (3.85 to 4.21 mL/g)	773:807	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	5	94	from	similar	762:768	arg1	N					836:836	2.04 to 2.64 N	823:836	2.04 to 2.64 N	823:836	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	5	94	from	similar	762:768	arg1	firmness					813:820	firmness	813:820	firmness (2.04 to 2.64 N)	813:837	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	0	95	theme	high-quality	34:45	arg1	flour					55:59	high-quality cassava flour	34:59	high-quality cassava flour	34:59	Quality attributes of breads from high-quality cassava flour improved with wet gluten.					
32691453	1	96	theme	cassava	205:211	arg1	PCF					220:222	PCF	220:222	PCF	220:222	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	1	96	theme	cassava	205:211	arg1	flour					213:217	preheated high-quality cassava flour	182:217	preheated high-quality cassava flour (PCF)	182:223	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	7	97	theme	crude	1066:1070	arg1	fiber					1072:1076	lower crude fiber	1060:1076	lower crude fiber	1060:1076	Wheat bread had significantly (P < 0.05) higher calorie, crude protein and crude fat, but lower crude fiber, ash, and carbohydrate compared to other bread samples.					
32691453	12	98	theme	making	1901:1906	arg1	bread					1895:1899	bread making	1895:1906	bread making	1895:1906	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	8	99	from	acceptability	1271:1283	arg1	different					1202:1210	different	1202:1210	different	1202:1210	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	8	99	from	acceptability	1271:1283	arg1	color					1243:1247	crust color	1237:1247	crust color	1237:1247	Sensory evaluation showed that bread from PCF was not significantly different from 100% wheat bread in crust color, texture, and overall acceptability but was impaired in flavor.					
32691453	9	100	theme	bread	1351:1355	arg1	feasibility					1336:1346	the feasibility	1332:1346	the feasibility of bread baking from preheated cassava flour with added gluten extract	1332:1417	The study revealed the feasibility of bread baking from preheated cassava flour with added gluten extract.					
32691453	4	101	theme	samples	475:481	arg1	contents					453:460	The moisture contents	440:460	The moisture contents of the flour samples	440:481	The moisture contents of the flour samples were in the range of 12.80 to 14.21%, and PCF exhibited water absorption capacity (1.12 mL/g) comparable to that of wheat flour (WF) (1.10 mL/g).					
32691453	10	102	theme	quality	1448:1454	arg1	attributes					1456:1465	some quality attributes	1443:1465	some quality attributes comparable to that of wheat bread	1443:1499	The bread produced had some quality attributes comparable to that of wheat bread.					
32691453	1	103	with	composite	233:241	arg1	flour					254:258	wheat flour	248:258	wheat flour	248:258	This study investigated the physical, chemical, and sensory attributes of breads produced from preheated high-quality cassava flour (PCF) and its composite with wheat flour (CWF).					
32691453	12	104	theme	nonwheat	1875:1882	arg1	flours					1884:1889	nonwheat flours	1875:1889	nonwheat flours	1875:1889	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	6	105	theme	gas	910:912	arg1	bubbles					914:920	gas bubbles	910:920	gas bubbles in the sample from PCF	910:943	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	5	106	from	volume	782:787	arg1	similar					762:768	similar	762:768	similar	762:768	There were significant (P < 0.05) differences in color characteristics, except in L* values and breads produced from WF and CWF were similar in specific volume (3.85 to 4.21 mL/g) and firmness (2.04 to 2.64 N).					
32691453	12	107	theme	viable	1844:1849	arg1	component					1851:1859	a viable component	1842:1859	a viable component to be used in nonwheat flours for bread making	1842:1906	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	12	107	theme	viable	1844:1849	arg1	extract					1831:1837	wheat gluten extract	1818:1837	wheat gluten extract	1818:1837	Findings from the study present wheat gluten extract as a viable component to be used in nonwheat flours for bread making.					
32691453	9	108	theme	cassava	1379:1385	arg1	flour					1387:1391	preheated cassava flour	1369:1391	preheated cassava flour with added gluten extract	1369:1417	The study revealed the feasibility of bread baking from preheated cassava flour with added gluten extract.					
32691453	9	109	with	flour	1387:1391	arg1	extract					1411:1417	added gluten extract	1398:1417	added gluten extract	1398:1417	The study revealed the feasibility of bread baking from preheated cassava flour with added gluten extract.					
32691453	6	110	from	bubbles	914:920	arg1	PCF					941:943	PCF	941:943	PCF	941:943	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32691453	6	110	from	bubbles	914:920	arg1	sample					929:934	the sample	925:934	the sample from PCF	925:943	Breads from WF and CWF exhibited similar crumb microstructure, though gas bubbles in the sample from PCF appeared less developed.					
32162086	0	0	theme	folic	113:117	arg1	acid					119:122	folic acid	113:122	folic acid	113:122	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	4	1	theme	composite	778:786	arg1	rate					798:801	scan rate	793:801	scan rate	793:801	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	1	theme	composite	778:786	arg1	amount					745:750	amount	745:750	amount of PtNPs-GNPs-MWCNTs-β-CD composite	745:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	1	theme	composite	778:786	arg1	composite					778:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	1	theme	composite	778:786	arg1	pH					741:742	pH	741:742	pH	741:742	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	1	2	theme	carbon	216:221	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	2	theme	carbon	216:221	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	2	theme	carbon	216:221	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	5	3	theme	linear	826:831	arg1	R2 = 0.9942					847:857	R2 = 0.9942	847:857	R2 = 0.9942	847:857	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	5	3	theme	linear	826:831	arg1	relationship					833:844	A good linear relationship	819:844	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1	819:959	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	2	4	theme	ultrasound-assisted	463:481	arg1	method					492:497	an ultrasound-assisted assembly method	460:497	an ultrasound-assisted assembly method	460:497	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	1	5	theme	nanotubes/β-cyclodextrin	223:246	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	5	theme	nanotubes/β-cyclodextrin	223:246	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	5	theme	nanotubes/β-cyclodextrin	223:246	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	7	6	from	sensitivity	1281:1291	arg1	samples					1343:1349	real samples	1338:1349	real samples	1338:1349	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	2	7	theme	PtNPs-GNPs-MWCNTs-β-CD	398:419	arg1	nanocomposite					421:433	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite	394:433	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite	394:433	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	0	8	theme	acid	119:122	arg1	detection					100:108	sensitive electrochemical detection	74:108	sensitive electrochemical detection of folic acid	74:122	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	5	9	located	observed	965:972	arg1	PtNPs-GNPs-MWCNTs-β-CD/GCE					977:1002	PtNPs-GNPs-MWCNTs-β-CD/GCE	977:1002	PtNPs-GNPs-MWCNTs-β-CD/GCE	977:1002	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	5	9	located	observed	965:972	arg2	R2 = 0.9942					847:857	R2 = 0.9942	847:857	R2 = 0.9942	847:857	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	5	9	located	observed	965:972	arg2	relationship					833:844	A good linear relationship	819:844	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1	819:959	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	7	10	theme	real	1338:1341	arg1	samples					1343:1349	real samples	1338:1349	real samples	1338:1349	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	3	11	theme	electrochemical	611:625	arg1	behavior					627:634	The electrochemical behavior	607:634	The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE	607:670	The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE was investigated in detail.					
32162086	5	12	theme	good	821:824	arg1	R2 = 0.9942					847:857	R2 = 0.9942	847:857	R2 = 0.9942	847:857	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	5	12	theme	good	821:824	arg1	relationship					833:844	A good linear relationship	819:844	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1	819:959	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	6	13	theme	detection	1009:1017	arg1	0.48 μmol L-1					1029:1041	0.48 μmol L-1	1029:1041	0.48 μmol L-1 (signal-to-noise ratio = 3)	1029:1069	The detection limit was 0.48 μmol L-1 (signal-to-noise ratio = 3).					
32162086	6	13	theme	detection	1009:1017	arg1	limit					1019:1023	The detection limit	1005:1023	The detection limit	1005:1023	The detection limit was 0.48 μmol L-1 (signal-to-noise ratio = 3).					
32162086	4	14	theme	experimental	709:720	arg1	rate					798:801	scan rate	793:801	scan rate	793:801	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	14	theme	experimental	709:720	arg1	amount					745:750	amount	745:750	amount of PtNPs-GNPs-MWCNTs-β-CD composite	745:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	14	theme	experimental	709:720	arg1	parameters					722:731	Some key experimental parameters	700:731	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate	700:801	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	14	theme	experimental	709:720	arg1	pH					741:742	pH	741:742	pH	741:742	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	14	theme	experimental	709:720	arg1	composite					778:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	5	15	theme	range	936:940	arg1	0.02-0.50 mmol L-1					942:959	the range 0.02-0.50 mmol L-1	932:959	the range 0.02-0.50 mmol L-1	932:959	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	1	16	theme	composite	248:256	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	16	theme	composite	248:256	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	16	theme	composite	248:256	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	2	17	theme	electron	580:587	arg1	microscopy					589:598	transmission electron microscopy	567:598	transmission electron microscopy (TEM)	567:604	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	2	17	theme	electron	580:587	arg1	TEM					601:603	TEM	601:603	TEM	601:603	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	7	18	contain	possessed	1255:1263	arg2	sensitivity					1281:1291	relatively high sensitivity	1265:1291	relatively high sensitivity	1265:1291	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	7	18	contain	possessed	1255:1263	arg1	electrode					1245:1253	the modified electrode	1232:1253	the modified electrode	1232:1253	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	7	18	contain	possessed	1255:1263	arg2	stability					1297:1305	stability	1297:1305	stability	1297:1305	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	7	19	theme	high	1276:1279	arg1	sensitivity					1281:1291	relatively high sensitivity	1265:1291	relatively high sensitivity	1265:1291	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	5	20	theme	cyclic	884:889	arg1	voltammetry					891:901	cyclic voltammetry	884:901	cyclic voltammetry (CV)	884:906	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	5	20	theme	cyclic	884:889	arg1	CV					904:905	CV	904:905	CV	904:905	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	7	21	theme	0.4 mg	1165:1170	arg1	FA					1172:1173	containing 0.4 mg FA	1154:1173	containing 0.4 mg FA per pill	1154:1182	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	3	22	theme	FA	639:640	arg1	behavior					627:634	The electrochemical behavior	607:634	The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE	607:670	The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE was investigated in detail.					
32162086	1	23	theme	PtNPs-GNPs-MWCNTs-β-CD	259:280	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	23	theme	PtNPs-GNPs-MWCNTs-β-CD	259:280	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	23	theme	PtNPs-GNPs-MWCNTs-β-CD	259:280	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	5	24	theme	voltammetry	891:901	arg1	current					873:879	peak current	868:879	peak current of cyclic voltammetry (CV)	868:906	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	5	24	theme	voltammetry	891:901	arg1	concentration					915:927	FA concentration	912:927	FA concentration	912:927	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	7	25	theme	modified	1236:1243	arg1	electrode					1245:1253	the modified electrode	1232:1253	the modified electrode	1232:1253	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	3	26	from	PtNPs-GNPs-MWCNTs-β-CD/GCE	645:670	arg1	behavior					627:634	The electrochemical behavior	607:634	The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE	607:670	The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE was investigated in detail.					
32162086	0	27	theme	glassy	46:51	arg1	electrode					60:68	modified glassy carbon electrode	37:68	modified glassy carbon electrode	37:68	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	4	28	dep	rate	798:801	arg1	scan					793:796	scan	793:796	scan	793:796	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	1	29	theme	modified	283:290	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	29	theme	modified	283:290	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	29	theme	modified	283:290	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	0	30	theme	modified	37:44	arg1	electrode					60:68	modified glassy carbon electrode	37:68	modified glassy carbon electrode	37:68	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	1	31	theme	sensitive	354:362	arg1	detection					364:372	the sensitive detection	350:372	the sensitive detection of folic acid (FA)	350:391	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	7	32	theme	containing	1154:1163	arg1	FA					1172:1173	containing 0.4 mg FA	1154:1173	containing 0.4 mg FA per pill	1154:1182	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	1	33	used	used	341:344	arg2	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	33	used	used	341:344	arg2	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	34	theme	novel	127:131	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	34	theme	novel	127:131	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	2	35	theme	electron	537:544	arg1	SEM					558:560	SEM	558:560	SEM	558:560	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	2	35	theme	electron	537:544	arg1	microscopy					546:555	scanning electron microscopy	528:555	scanning electron microscopy (SEM)	528:561	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	1	36	theme	carbon	292:297	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	36	theme	carbon	292:297	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	36	theme	carbon	292:297	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	7	37	theme	FA	1144:1145	arg1	pills					1147:1151	FA pills	1144:1151	FA pills (containing 0.4 mg FA per pill)	1144:1183	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	5	38	theme	FA	912:913	arg1	concentration					915:927	FA concentration	912:927	FA concentration	912:927	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	7	39	dep	pills	1147:1151	arg1	FA					1172:1173	containing 0.4 mg FA	1154:1173	containing 0.4 mg FA per pill	1154:1182	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	1	40	theme	glass	299:303	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	40	theme	glass	299:303	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	40	theme	glass	299:303	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	0	41	theme	carbon	53:58	arg1	electrode					60:68	modified glassy carbon electrode	37:68	modified glassy carbon electrode	37:68	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	6	42	theme	signal-to-noise	1044:1058	arg1	0.48 μmol L-1					1029:1041	0.48 μmol L-1	1029:1041	0.48 μmol L-1 (signal-to-noise ratio = 3)	1029:1069	The detection limit was 0.48 μmol L-1 (signal-to-noise ratio = 3).					
32162086	6	42	theme	signal-to-noise	1044:1058	arg1	ratio = 3					1060:1068	signal-to-noise ratio = 3	1044:1068	signal-to-noise ratio = 3	1044:1068	The detection limit was 0.48 μmol L-1 (signal-to-noise ratio = 3).					
32162086	5	43	theme	peak	868:871	arg1	current					873:879	peak current	868:879	peak current of cyclic voltammetry (CV)	868:906	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	7	44	from	stability	1297:1305	arg1	samples					1343:1349	real samples	1338:1349	real samples	1338:1349	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	2	45	theme	assembly	483:490	arg1	method					492:497	an ultrasound-assisted assembly method	460:497	an ultrasound-assisted assembly method	460:497	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	0	46	theme	sensitive	74:82	arg1	detection					100:108	sensitive electrochemical detection	74:108	sensitive electrochemical detection of folic acid	74:122	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	1	47	theme	folic	377:381	arg1	FA					389:390	FA	389:390	FA	389:390	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	47	theme	folic	377:381	arg1	acid					383:386	folic acid	377:386	folic acid (FA)	377:391	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	7	48	theme	FA	1138:1139	arg1	determination					1121:1133	the determination	1117:1133	the determination of FA	1117:1139	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	7	49	theme	FA	1332:1333	arg1	determination					1315:1327	the determination	1311:1327	the determination of FA	1311:1333	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	5	50	from	concentration	915:927	arg1	0.02-0.50 mmol L-1					942:959	the range 0.02-0.50 mmol L-1	932:959	the range 0.02-0.50 mmol L-1	932:959	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	1	51	theme	electrochemical	133:147	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	51	theme	electrochemical	133:147	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	2	52	theme	scanning	528:535	arg1	SEM					558:560	SEM	558:560	SEM	558:560	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	2	52	theme	scanning	528:535	arg1	microscopy					546:555	scanning electron microscopy	528:555	scanning electron microscopy (SEM)	528:561	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	1	53	theme	acid	383:386	arg1	detection					364:372	the sensitive detection	350:372	the sensitive detection of folic acid (FA)	350:391	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	4	54	theme	PtNPs-GNPs-MWCNTs-β-CD	755:776	arg1	composite					778:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	1	55	theme	platinum	157:164	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	55	theme	platinum	157:164	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	55	theme	platinum	157:164	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	2	56	theme	transmission	567:578	arg1	microscopy					589:598	transmission electron microscopy	567:598	transmission electron microscopy (TEM)	567:604	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	2	56	theme	transmission	567:578	arg1	TEM					601:603	TEM	601:603	TEM	601:603	The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).					
32162086	7	57	theme	%	1098:1098	arg1	recovery					1074:1081	A recovery	1072:1081	A recovery of 97.55-102.96%	1072:1098	A recovery of 97.55-102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples.					
32162086	1	58	theme	nanoparticles/graphene	166:187	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	58	theme	nanoparticles/graphene	166:187	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	58	theme	nanoparticles/graphene	166:187	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	4	59	theme	key	705:707	arg1	rate					798:801	scan rate	793:801	scan rate	793:801	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	59	theme	key	705:707	arg1	amount					745:750	amount	745:750	amount of PtNPs-GNPs-MWCNTs-β-CD composite	745:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	59	theme	key	705:707	arg1	parameters					722:731	Some key experimental parameters	700:731	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate	700:801	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	59	theme	key	705:707	arg1	pH					741:742	pH	741:742	pH	741:742	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	4	59	theme	key	705:707	arg1	composite					778:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	PtNPs-GNPs-MWCNTs-β-CD composite	755:786	Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized.					
32162086	0	60	theme	electrochemical	84:98	arg1	detection					100:108	sensitive electrochemical detection	74:108	sensitive electrochemical detection of folic acid	74:122	PtNPs-GNPs-MWCNTs-β-CD nanocomposite modified glassy carbon electrode for sensitive electrochemical detection of folic acid.					
32162086	5	61	from	current	873:879	arg1	0.02-0.50 mmol L-1					942:959	the range 0.02-0.50 mmol L-1	932:959	the range 0.02-0.50 mmol L-1	932:959	A good linear relationship (R2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02-0.50 mmol L-1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE.					
32162086	1	62	theme	nanoplatelets/multi-walled	189:214	arg1	sensor					149:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor	125:154	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	62	theme	nanoplatelets/multi-walled	189:214	arg1	GCE					316:318	GCE	316:318	GCE	316:318	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
32162086	1	62	theme	nanoplatelets/multi-walled	189:214	arg1	electrode					305:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode	157:313	platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE)	157:319	A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA).					
33379036	8	0	gly	glycoproteins	1902:1914	arg1	glycoproteins					1902:1914	11 glycoproteins	1899:1914	11 glycoproteins	1899:1914	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	1	1	contain	contains	187:194	arg1	milk					182:185	Human milk	176:185	Human milk	176:185	Human milk contains numerous N-glycoproteins with functions that provide protection to the infant.					
33379036	1	1	contain	contains	187:194	arg2	N-glycoproteins					205:219	numerous N-glycoproteins	196:219	numerous N-glycoproteins with functions that provide protection to the infant	196:272	Human milk contains numerous N-glycoproteins with functions that provide protection to the infant.					
33379036	6	2	theme	glycosylation	1457:1469	arg1	site					1471:1474	glycosylation site	1457:1474	glycosylation site	1457:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	2	3	theme	human	326:330	arg1	glycoproteins					337:349	human milk glycoproteins	326:349	human milk glycoproteins	326:349	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	6	4	theme	fragment	1352:1359	arg1	ions					1361:1364	fragment ions	1352:1364	fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site	1352:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	6	5	theme	sequence	1444:1451	arg1	identification					1394:1407	effective identification	1384:1407	effective identification of the glycan composition, peptide sequence and glycosylation site	1384:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	2	6	theme	role	318:321	arg1	understanding					286:298	Increasing understanding	275:298	Increasing understanding of the functional role of human milk glycoproteins within the infant	275:367	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	5	7	theme	tryptic	988:994	arg1	enrichment					1009:1018	tryptic glycopeptide enrichment	988:1018	tryptic glycopeptide enrichment	988:1018	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	7	8	theme	milk	1599:1602	arg1	profiling					1619:1627	human milk N-glycopeptide profiling	1593:1627	human milk N-glycopeptide profiling	1593:1627	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	4	9	theme	liquid	717:722	arg1	spectrometry					744:755	liquid chromatography/mass spectrometry	717:755	liquid chromatography/mass spectrometry	717:755	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	2	10	theme	glycoproteins	337:349	arg1	role					318:321	the functional role	303:321	the functional role of human milk glycoproteins within the infant	303:367	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	8	11	gly	N-glycoproteins	1722:1736	arg1	N-glycoproteins					1722:1736	precursor N-glycoproteins	1712:1736	precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins)	1712:1780	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	9	12	theme	human	1949:1953	arg1	N-glycoproteins					1960:1974	human milk N-glycoproteins	1949:1974	human milk N-glycoproteins provided by this study	1949:1997	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	6	13	theme	composition	1423:1433	arg1	identification					1394:1407	effective identification	1384:1407	effective identification of the glycan composition, peptide sequence and glycosylation site	1384:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	0	14	theme	milk	154:157	arg1	N-glycoproteins					159:173	human milk N-glycoproteins	148:173	human milk N-glycoproteins	148:173	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	5	15	theme	molecular	933:941	arg1	filtration					958:967	molecular weight cut-off filtration	933:967	molecular weight cut-off filtration	933:967	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	16	theme	non-target	788:797	arg1	molecules					799:807	non-target molecules	788:807	non-target molecules	788:807	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	17	theme	graphitized	1044:1054	arg1	carbon					1056:1061	porous graphitized carbon	1037:1061	porous graphitized carbon	1037:1061	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	8	18	with	approach	1821:1828	arg1	extraction					1846:1855	protein extraction	1838:1855	protein extraction	1838:1855	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	8	18	with	approach	1821:1828	arg1	clean-up					1865:1872	C18 clean-up	1861:1872	C18 clean-up	1861:1872	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	0	19	gly	N-glycoproteins	159:173	arg1	N-glycoproteins					159:173	human milk N-glycoproteins	148:173	human milk N-glycoproteins	148:173	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	8	20	theme	N-glycopeptides	1692:1706	arg1	number					1682:1687	the number	1678:1687	the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins)	1678:1780	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	0	21	theme	enrichment	71:80	arg1	examination					11:21	Systematic examination	0:21	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.	0:174	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	1	22	theme	Human	176:180	arg1	milk					182:185	Human milk	176:185	Human milk	176:185	Human milk contains numerous N-glycoproteins with functions that provide protection to the infant.					
33379036	8	23	theme	precursor	1712:1720	arg1	N-glycoproteins					1722:1736	precursor N-glycoproteins	1712:1736	precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins)	1712:1780	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	0	24	theme	MS/MS	86:90	arg1	techniques					106:115	MS/MS fragmentation techniques	86:115	MS/MS fragmentation techniques	86:115	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	7	25	theme	EThcD-fragmentation	1546:1564	arg1	combination					1497:1507	a combination	1495:1507	a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation	1495:1564	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	7	25	theme	EThcD-fragmentation	1546:1564	arg1	effective					1579:1587	effective	1579:1587	effective	1579:1587	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	5	26	dep	carbon	1056:1061	arg1	SPE					1145:1147	SPE	1145:1147	SPE	1145:1147	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	26	dep	carbon	1056:1061	arg1	extraction					1133:1142	-solid phase extraction	1120:1142	-solid phase extraction (SPE)	1120:1148	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	0	27	theme	techniques	106:115	arg1	examination					11:21	Systematic examination	0:21	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.	0:174	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	9	28	gly	glycoproteins	2062:2074	arg1	glycoproteins					2062:2074	glycoproteins	2062:2074	glycoproteins	2062:2074	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	8	29	gly	246 N-glycopeptides	1739:1757	arg1	glycoproteins					1767:1779	29 glycoproteins	1764:1779	29 glycoproteins	1764:1779	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	8	29	gly	246 N-glycopeptides	1739:1757	arg2	246 N-glycopeptides					1739:1757	246 N-glycopeptides	1739:1757	246 N-glycopeptides from 29 glycoproteins	1739:1779	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	0	30	theme	site-specific	121:133	arg1	profiling					135:143	site-specific profiling	121:143	site-specific profiling of human milk N-glycoproteins	121:173	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	5	31	theme	-solid	1120:1125	arg1	SPE					1145:1147	SPE	1145:1147	SPE	1145:1147	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	31	theme	-solid	1120:1125	arg1	extraction					1133:1142	-solid phase extraction	1120:1142	-solid phase extraction (SPE)	1120:1148	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	7	32	theme	precipitation	1517:1529	arg1	combination					1497:1507	a combination	1495:1507	a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation	1495:1564	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	7	32	theme	precipitation	1517:1529	arg1	effective					1579:1587	effective	1579:1587	effective	1579:1587	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	3	33	theme	glycosylation	503:515	arg1	analysis					517:524	site-specific glycosylation analysis	489:524	site-specific glycosylation analysis across the entire human milk proteome	489:562	However, optimized methods for site-specific glycosylation analysis across the entire human milk proteome are not available.					
33379036	0	34	theme	Systematic	0:9	arg1	examination					11:21	Systematic examination	0:21	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.	0:174	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	5	35	theme	interaction	1079:1089	arg1	HILIC					1114:1118	HILIC	1114:1118	HILIC	1114:1118	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	35	theme	interaction	1079:1089	arg1	chromatography					1098:1111	hydrophilic interaction liquid chromatography	1067:1111	hydrophilic interaction liquid chromatography (HILIC)	1067:1119	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	4	36	theme	techniques	632:641	arg1	analysis					620:627	a systematic analysis	607:627	a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk	607:709	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	3	37	theme	milk	550:553	arg1	proteome					555:562	the entire human milk proteome	533:562	the entire human milk proteome	533:562	However, optimized methods for site-specific glycosylation analysis across the entire human milk proteome are not available.					
33379036	5	38	theme	weight	943:948	arg1	filtration					958:967	molecular weight cut-off filtration	933:967	molecular weight cut-off filtration	933:967	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	3	39	theme	entire	537:542	arg1	proteome					555:562	the entire human milk proteome	533:562	the entire human milk proteome	533:562	However, optimized methods for site-specific glycosylation analysis across the entire human milk proteome are not available.					
33379036	0	40	theme	extraction	34:43	arg1	examination					11:21	Systematic examination	0:21	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.	0:174	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	3	41	theme	site-specific	489:501	arg1	analysis					517:524	site-specific glycosylation analysis	489:524	site-specific glycosylation analysis across the entire human milk proteome	489:562	However, optimized methods for site-specific glycosylation analysis across the entire human milk proteome are not available.					
33379036	7	42	theme	methods	1486:1492	arg1	combination					1497:1507	a combination	1495:1507	a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation	1495:1564	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	7	42	theme	methods	1486:1492	arg1	effective					1579:1587	effective	1579:1587	effective	1579:1587	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	3	43	theme	optimized	467:475	arg1	methods					477:483	optimized methods	467:483	optimized methods for site-specific glycosylation analysis across the entire human milk proteome	467:562	However, optimized methods for site-specific glycosylation analysis across the entire human milk proteome are not available.					
33379036	8	44	theme	extraction	1810:1819	arg1	approach					1821:1828	a more common extraction approach	1796:1828	a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins)	1796:1915	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	5	45	theme	acid	913:916	arg1	precipitation					918:930	trichloroacetic acid precipitation	897:930	trichloroacetic acid precipitation	897:930	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	6	46	theme	collision	1310:1318	arg1	EThcD					1334:1338	EThcD	1334:1338	EThcD	1334:1338	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	6	46	theme	collision	1310:1318	arg1	dissociation					1320:1331	electron-transfer/higher-energy collision dissociation	1278:1331	electron-transfer/higher-energy collision dissociation (EThcD)	1278:1339	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	5	47	from	molecules	799:807	arg1	interference					770:781	interference	770:781	interference from non-target molecules	770:807	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	1	48	with	N-glycoproteins	205:219	arg1	functions					226:234	functions	226:234	functions that provide protection to the infant	226:272	Human milk contains numerous N-glycoproteins with functions that provide protection to the infant.					
33379036	4	49	theme	N-glycans	687:695	arg1	compositions					671:682	compositions	671:682	compositions	671:682	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	4	49	theme	N-glycans	687:695	arg1	sites					661:665	sites	661:665	sites	661:665	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	4	49	theme	N-glycans	687:695	arg1	N-glycans					687:695	N-glycans	687:695	N-glycans	687:695	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	8	50	theme	optimized	1635:1643	arg1	approach					1645:1652	This optimized approach	1630:1652	This optimized approach	1630:1652	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	2	51	theme	glycosylation	434:446	arg1	patterns					448:455	their site-specific glycosylation patterns	414:455	their site-specific glycosylation patterns	414:455	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	0	52	theme	glycopeptide	58:69	arg1	enrichment					71:80	proteolytic glycopeptide enrichment	46:80	proteolytic glycopeptide enrichment	46:80	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	0	53	gly	glycopeptide	58:69	arg2	glycopeptide					58:69	proteolytic glycopeptide enrichment	46:80	proteolytic glycopeptide enrichment	46:80	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	6	54	theme	electron-transfer	1243:1259	arg1	dissociation					1261:1272	electron-transfer dissociation	1243:1272	electron-transfer dissociation	1243:1272	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	4	55	theme	human	700:704	arg1	milk					706:709	human milk	700:709	human milk	700:709	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	2	56	gly	glycoproteins	337:349	arg1	glycoproteins					337:349	human milk glycoproteins	326:349	human milk glycoproteins	326:349	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	1	57	gly	N-glycoproteins	205:219	arg1	N-glycoproteins					205:219	numerous N-glycoproteins	196:219	numerous N-glycoproteins with functions that provide protection to the infant	196:272	Human milk contains numerous N-glycoproteins with functions that provide protection to the infant.					
33379036	6	58	theme	dissociation	1229:1240	arg1	capacity					1193:1200	the capacity	1189:1200	the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site	1189:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	8	59	theme	C18	1861:1863	arg1	clean-up					1865:1872	C18 clean-up	1861:1872	C18 clean-up	1861:1872	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	6	60	theme	site	1471:1474	arg1	identification					1394:1407	effective identification	1384:1407	effective identification of the glycan composition, peptide sequence and glycosylation site	1384:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	2	61	theme	milk	332:335	arg1	glycoproteins					337:349	human milk glycoproteins	326:349	human milk glycoproteins	326:349	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	8	62	dep	N-glycoproteins	1722:1736	arg1	246 N-glycopeptides					1739:1757	246 N-glycopeptides	1739:1757	246 N-glycopeptides from 29 glycoproteins	1739:1779	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	5	63	gly	glycopeptide	996:1007	arg2	glycopeptide					996:1007	tryptic glycopeptide enrichment	988:1018	tryptic glycopeptide enrichment	988:1018	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	8	64	gly	glycoproteins	1767:1779	arg1	glycoproteins					1767:1779	29 glycoproteins	1764:1779	29 glycoproteins	1764:1779	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	1	65	theme	numerous	196:203	arg1	N-glycoproteins					205:219	numerous N-glycoproteins	196:219	numerous N-glycoproteins with functions that provide protection to the infant	196:272	Human milk contains numerous N-glycoproteins with functions that provide protection to the infant.					
33379036	0	66	theme	proteolytic	46:56	arg1	enrichment					71:80	proteolytic glycopeptide enrichment	46:80	proteolytic glycopeptide enrichment	46:80	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	9	67	theme	breast	2087:2092	arg1	infant					2103:2108	the breast milk-fed infant	2083:2108	the breast milk-fed infant	2083:2108	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	6	68	theme	peptide	1436:1442	arg1	sequence					1444:1451	peptide sequence	1436:1451	peptide sequence	1436:1451	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	5	69	theme	glycopeptide	996:1007	arg1	enrichment					1009:1018	tryptic glycopeptide enrichment	988:1018	tryptic glycopeptide enrichment	988:1018	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	9	70	from	advancement	1922:1932	arg1	methods					1937:1943	methods	1937:1943	methods for human milk N-glycoproteins provided by this study	1937:1997	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	7	71	theme	N-glycopeptide	1604:1617	arg1	profiling					1619:1627	human milk N-glycopeptide profiling	1593:1627	human milk N-glycopeptide profiling	1593:1627	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	0	72	theme	human	148:152	arg1	N-glycoproteins					159:173	human milk N-glycoproteins	148:173	human milk N-glycoproteins	148:173	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	9	73	gly	N-glycoproteins	1960:1974	arg1	N-glycoproteins					1960:1974	human milk N-glycoproteins	1949:1974	human milk N-glycoproteins provided by this study	1949:1997	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	5	74	theme	cut-off	950:956	arg1	filtration					958:967	molecular weight cut-off filtration	933:967	molecular weight cut-off filtration	933:967	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	8	75	from	glycoproteins	1767:1779	arg1	246 N-glycopeptides					1739:1757	246 N-glycopeptides	1739:1757	246 N-glycopeptides from 29 glycoproteins	1739:1779	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	7	76	theme	human	1593:1597	arg1	profiling					1619:1627	human milk N-glycopeptide profiling	1593:1627	human milk N-glycopeptide profiling	1593:1627	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	0	77	theme	N-glycoproteins	159:173	arg1	profiling					135:143	site-specific profiling	121:143	site-specific profiling of human milk N-glycoproteins	121:173	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	2	78	theme	Increasing	275:284	arg1	understanding					286:298	Increasing understanding	275:298	Increasing understanding of the functional role of human milk glycoproteins within the infant	275:367	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	8	79	gly	N-glycopeptides	1692:1706	arg2	N-glycopeptides					1692:1706	N-glycopeptides	1692:1706	N-glycopeptides	1692:1706	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	9	80	theme	milk	1955:1958	arg1	N-glycoproteins					1960:1974	human milk N-glycoproteins	1949:1974	human milk N-glycoproteins provided by this study	1949:1997	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	6	81	theme	glycan	1416:1421	arg1	composition					1423:1433	the glycan composition	1412:1433	the glycan composition	1412:1433	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	4	82	theme	chromatography/mass	724:742	arg1	spectrometry					744:755	liquid chromatography/mass spectrometry	717:755	liquid chromatography/mass spectrometry	717:755	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	4	83	dep	sites	661:665	arg1	the					657:659	the	657:659	the	657:659	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	5	84	theme	porous	1037:1042	arg1	carbon					1056:1061	porous graphitized carbon	1037:1061	porous graphitized carbon	1037:1061	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	2	85	theme	functional	307:316	arg1	role					318:321	the functional role	303:321	the functional role of human milk glycoproteins within the infant	303:367	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
33379036	6	86	theme	effective	1384:1392	arg1	identification					1394:1407	effective identification	1384:1407	effective identification of the glycan composition, peptide sequence and glycosylation site	1384:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	5	87	theme	ethanol	867:873	arg1	precipitation					882:894	ethanol (EtOH) precipitation	867:894	ethanol (EtOH) precipitation	867:894	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	8	88	theme	N-glycoproteins	1722:1736	arg1	number					1682:1687	the number	1678:1687	the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins)	1678:1780	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	7	89	theme	HILIC-SPE	1532:1540	arg1	combination					1497:1507	a combination	1495:1507	a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation	1495:1564	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	7	89	theme	HILIC-SPE	1532:1540	arg1	effective					1579:1587	effective	1579:1587	effective	1579:1587	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	0	90	theme	fragmentation	92:104	arg1	techniques					106:115	MS/MS fragmentation techniques	86:115	MS/MS fragmentation techniques	86:115	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	6	91	gly	glycosylation	1457:1469	arg2	site					1471:1474	glycosylation site	1457:1474	glycosylation site	1457:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	9	92	theme	key	2012:2014	arg1	step					2016:2019	a key step	2010:2019	a key step for better understanding the function of glycoproteins within the breast milk-fed infant	2010:2108	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	5	93	theme	protein	837:843	arg1	extraction					845:854	protein extraction	837:854	protein extraction	837:854	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	8	94	from	glycoproteins	1902:1914	arg1	62 N-glycopeptides					1875:1892	62 N-glycopeptides	1875:1892	62 N-glycopeptides from 11 glycoproteins	1875:1914	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	8	95	dep	extraction	1846:1855	arg1	62 N-glycopeptides					1875:1892	62 N-glycopeptides	1875:1892	62 N-glycopeptides from 11 glycoproteins	1875:1914	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	6	96	theme	collision	1219:1227	arg1	dissociation					1229:1240	higher-energy collision dissociation	1205:1240	higher-energy collision dissociation	1205:1240	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	5	97	theme	hydrophilic	1067:1077	arg1	HILIC					1114:1118	HILIC	1114:1118	HILIC	1114:1118	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	97	theme	hydrophilic	1067:1077	arg1	chromatography					1098:1111	hydrophilic interaction liquid chromatography	1067:1111	hydrophilic interaction liquid chromatography (HILIC)	1067:1119	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	0	98	theme	protein	26:32	arg1	extraction					34:43	protein extraction	26:43	protein extraction	26:43	Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins.					
33379036	8	99	theme	common	1803:1808	arg1	approach					1821:1828	a more common extraction approach	1796:1828	a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins)	1796:1915	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	5	100	theme	liquid	1091:1096	arg1	HILIC					1114:1118	HILIC	1114:1118	HILIC	1114:1118	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	100	theme	liquid	1091:1096	arg1	chromatography					1098:1111	hydrophilic interaction liquid chromatography	1067:1111	hydrophilic interaction liquid chromatography (HILIC)	1067:1119	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	9	101	theme	glycoproteins	2062:2074	arg1	function					2050:2057	the function	2046:2057	the function of glycoproteins	2046:2074	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	7	102	theme	EtOH	1512:1515	arg1	precipitation					1517:1529	EtOH precipitation	1512:1529	EtOH precipitation	1512:1529	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	4	103	theme	systematic	609:618	arg1	analysis					620:627	a systematic analysis	607:627	a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk	607:709	Therefore, we performed a systematic analysis of techniques for profiling the sites and compositions of N-glycans in human milk using liquid chromatography/mass spectrometry.					
33379036	3	104	theme	human	544:548	arg1	proteome					555:562	the entire human milk proteome	533:562	the entire human milk proteome	533:562	However, optimized methods for site-specific glycosylation analysis across the entire human milk proteome are not available.					
33379036	5	105	theme	trichloroacetic	897:911	arg1	precipitation					918:930	trichloroacetic acid precipitation	897:930	trichloroacetic acid precipitation	897:930	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	106	theme	acetone	1154:1160	arg1	precipitation					1162:1174	acetone precipitation	1154:1174	acetone precipitation	1154:1174	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	6	107	theme	dissociation	1320:1331	arg1	capacity					1193:1200	the capacity	1189:1200	the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site	1189:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	9	108	theme	milk-fed	2094:2101	arg1	infant					2103:2108	the breast milk-fed infant	2083:2108	the breast milk-fed infant	2083:2108	The advancement in methods for human milk N-glycoproteins provided by this study represents a key step for better understanding the function of glycoproteins within the breast milk-fed infant.					
33379036	8	109	gly	62 N-glycopeptides	1875:1892	arg2	62 N-glycopeptides					1875:1892	62 N-glycopeptides	1875:1892	62 N-glycopeptides from 11 glycoproteins	1875:1914	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	8	109	gly	62 N-glycopeptides	1875:1892	arg1	glycoproteins					1902:1914	11 glycoproteins	1899:1914	11 glycoproteins	1899:1914	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	5	110	theme	phase	1127:1131	arg1	SPE					1145:1147	SPE	1145:1147	SPE	1145:1147	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	5	110	theme	phase	1127:1131	arg1	extraction					1133:1142	-solid phase extraction	1120:1142	-solid phase extraction (SPE)	1120:1148	To decrease interference from non-target molecules, we compared techniques for protein extraction, including ethanol (EtOH) precipitation, trichloroacetic acid precipitation, molecular weight cut-off filtration and techniques for tryptic glycopeptide enrichment, including C18-, porous graphitized carbon and hydrophilic interaction liquid chromatography (HILIC)-solid phase extraction (SPE) and acetone precipitation.					
33379036	6	111	theme	electron-transfer/higher-energy	1278:1308	arg1	EThcD					1334:1338	EThcD	1334:1338	EThcD	1334:1338	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	6	111	theme	electron-transfer/higher-energy	1278:1308	arg1	dissociation					1320:1331	electron-transfer/higher-energy collision dissociation	1278:1331	electron-transfer/higher-energy collision dissociation (EThcD)	1278:1339	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	8	112	theme	protein	1838:1844	arg1	extraction					1846:1855	protein extraction	1838:1855	protein extraction	1838:1855	This optimized approach significantly increased the number of N-glycopeptides and precursor N-glycoproteins (246 N-glycopeptides from 29 glycoproteins) compared with a more common extraction approach with no protein extraction and C18 clean-up (62 N-glycopeptides from 11 glycoproteins).					
33379036	7	113	gly	N-glycopeptide	1604:1617	arg2	N-glycopeptide					1604:1617	human milk N-glycopeptide profiling	1593:1627	human milk N-glycopeptide profiling	1593:1627	Of these methods, a combination of EtOH precipitation, HILIC-SPE and EThcD-fragmentation was the most effective for human milk N-glycopeptide profiling.					
33379036	6	114	theme	dissociation	1261:1272	arg1	capacity					1193:1200	the capacity	1189:1200	the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site	1189:1474	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	6	115	theme	higher-energy	1205:1217	arg1	dissociation					1229:1240	higher-energy collision dissociation	1205:1240	higher-energy collision dissociation	1205:1240	We compared the capacity of higher-energy collision dissociation, electron-transfer dissociation and electron-transfer/higher-energy collision dissociation (EThcD) to produce fragment ions that would enable effective identification of the glycan composition, peptide sequence and glycosylation site.					
33379036	2	116	theme	site-specific	420:432	arg1	patterns					448:455	their site-specific glycosylation patterns	414:455	their site-specific glycosylation patterns	414:455	Increasing understanding of the functional role of human milk glycoproteins within the infant requires toolsets to comprehensively profile their site-specific glycosylation patterns.					
32470591	7	0	theme	nanocomposite	1299:1311	arg1	packaging					1318:1326	the ZnO/CIN/CMC nanocomposite film packaging	1283:1326	the ZnO/CIN/CMC nanocomposite film packaging	1283:1326	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	5	1	theme	excellent	949:957	arg1	capacities					967:976	excellent barrier capacities	949:976	excellent barrier capacities against water and oxygen molecules	949:1011	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	1	2	theme	zinc	269:272	arg1	oxide					274:278	zinc oxide	269:278	zinc oxide	269:278	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	0	3	theme	composite	93:101	arg1	coating					103:109	a carboxymethylcellulose-based composite coating	62:109	a carboxymethylcellulose-based composite coating	62:109	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	4	4	theme	antifungal	726:735	arg1	performance					737:747	antifungal performance	726:747	antifungal performance	726:747	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	1	5	theme	oxide	274:278	arg1	ZnONPs					295:300	ZnONPs	295:300	ZnONPs	295:300	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	1	5	theme	oxide	274:278	arg1	nanoparticles					280:292	plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles	230:292	plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis	230:321	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	3	6	theme	ZnONPs	559:564	arg1	incorporation					566:578	the ZnONPs incorporation	555:578	the ZnONPs incorporation	555:578	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	5	7	theme	anti-Aspergillus	1017:1032	arg1	activity					1040:1047	anti-Aspergillus niger activity	1017:1047	anti-Aspergillus niger activity	1017:1047	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	5	8	theme	niger	1034:1038	arg1	activity					1040:1047	anti-Aspergillus niger activity	1017:1047	anti-Aspergillus niger activity	1017:1047	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	5	9	theme	oxygen	996:1001	arg1	molecules					1003:1011	oxygen molecules	996:1011	oxygen molecules	996:1011	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	7	10	theme	film	1313:1316	arg1	packaging					1318:1326	the ZnO/CIN/CMC nanocomposite film packaging	1283:1326	the ZnO/CIN/CMC nanocomposite film packaging	1283:1326	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	0	11	theme	postharvest	126:136	arg1	quality					138:144	the postharvest quality	122:144	the postharvest quality of cherry tomatoes	122:163	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	6	12	theme	weight	1137:1142	arg1	loss					1144:1147	weight loss	1137:1147	weight loss	1137:1147	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	6	13	dep	loss	1144:1147	arg1	the					1133:1135	the	1133:1135	the	1133:1135	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	4	14	theme	barrier	705:711	arg1	capacity					713:720	water barrier capacity	699:720	water barrier capacity	699:720	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	2	15	theme	antifungal	407:416	arg1	CMC					468:470	pristine CMC	459:470	pristine CMC	459:470	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	15	theme	antifungal	407:416	arg1	activities					418:427	antifungal activities	407:427	antifungal activities	407:427	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	5	16	theme	CMC-based	822:830	arg1	film					842:845	CMC-based composite film	822:845	CMC-based composite film incorporating with both CIN and ZnONPs	822:884	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	4	17	theme	CIN	817:819	arg1	addition					805:812	the addition	801:812	the addition of CIN	801:819	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	6	18	theme	tomatoes	1172:1179	arg1	firmness					1153:1160	firmness	1153:1160	firmness	1153:1160	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	6	18	theme	tomatoes	1172:1179	arg1	loss					1144:1147	weight loss	1137:1147	weight loss	1137:1147	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	1	19	theme	-based	194:199	arg1	packaging					206:214	Carboxymethylcellulose (CMC)-based film packaging	166:214	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis	166:321	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	6	20	dep	effective	1109:1117	arg1	inhibiting					1122:1131	inhibiting	1122:1131	inhibiting the weight loss and firmness of cherry tomatoes	1122:1179	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	6	20	dep	effective	1109:1117	arg1	decreasing					1188:1197	decreasing	1188:1197	decreasing the total acidity content of these fruits after storage	1188:1253	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	6	21	theme	cherry	1165:1170	arg1	tomatoes					1172:1179	cherry tomatoes	1165:1179	cherry tomatoes	1165:1179	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	3	22	theme	nanocomposite	591:603	arg1	film					605:608	a nanocomposite film	589:608	a nanocomposite film with low transparency, good flexibility and high mechanical resistance	589:679	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	1	23	theme	film	201:204	arg1	packaging					206:214	Carboxymethylcellulose (CMC)-based film packaging	166:214	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis	166:321	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	2	24	theme	pristine	459:466	arg1	CIN/CMC					473:479	CIN/CMC	473:479	CIN/CMC	473:479	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	24	theme	pristine	459:466	arg1	CMC					468:470	pristine CMC	459:470	pristine CMC	459:470	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	24	theme	pristine	459:466	arg1	ZnONPs/CIN/CMC					494:507	ZnONPs/CIN/CMC	494:507	ZnONPs/CIN/CMC	494:507	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	24	theme	pristine	459:466	arg1	ZnONPs/CMC					482:491	ZnONPs/CMC	482:491	ZnONPs/CMC	482:491	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	6	25	theme	fruits	1234:1239	arg1	content					1217:1223	the total acidity content	1199:1223	the total acidity content of these fruits	1199:1239	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	2	26	theme	barrier	384:390	arg1	CMC					468:470	pristine CMC	459:470	pristine CMC	459:470	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	26	theme	barrier	384:390	arg1	properties					392:401	the physico-mechanical and barrier properties	357:401	properties	392:401	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	7	27	theme	storage	1455:1461	arg1	period					1463:1468	postharvest storage period	1443:1468	postharvest storage period	1443:1468	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	2	28	theme	films	442:446	arg1	CMC					468:470	pristine CMC	459:470	pristine CMC	459:470	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	28	theme	films	442:446	arg1	properties					392:401	the physico-mechanical and barrier properties	357:401	properties	392:401	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	28	theme	films	442:446	arg1	activities					418:427	antifungal activities	407:427	antifungal activities	407:427	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	0	29	theme	cinnamaldehyde	15:28	arg1	Utilization					0:10	Utilization	0:10	Utilization of cinnamaldehyde and zinc oxide	0:43	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	5	30	theme	barrier	959:965	arg1	capacities					967:976	excellent barrier capacities	949:976	excellent barrier capacities against water and oxygen molecules	949:1011	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	2	31	theme	CMC-based	432:440	arg1	films					442:446	CMC-based films	432:446	CMC-based films	432:446	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	0	32	theme	oxide	39:43	arg1	Utilization					0:10	Utilization	0:10	Utilization of cinnamaldehyde and zinc oxide	0:43	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	1	33	theme	simple	306:311	arg1	synthesis					313:321	simple synthesis	306:321	simple synthesis	306:321	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	0	34	theme	tomatoes	156:163	arg1	quality					138:144	the postharvest quality	122:144	the postharvest quality of cherry tomatoes	122:163	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	4	35	theme	water	699:703	arg1	capacity					713:720	water barrier capacity	699:720	water barrier capacity	699:720	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	1	36	theme	plant-sourced	230:242	arg1	CIN					260:262	CIN	260:262	CIN	260:262	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	1	36	theme	plant-sourced	230:242	arg1	cinnamaldehyde					244:257	plant-sourced cinnamaldehyde	230:257	plant-sourced cinnamaldehyde (CIN)	230:263	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	0	37	theme	zinc	34:37	arg1	oxide					39:43	zinc oxide	34:43	zinc oxide	34:43	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	1	38	theme	synthesis	313:321	arg1	ZnONPs					295:300	ZnONPs	295:300	ZnONPs	295:300	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	1	38	theme	synthesis	313:321	arg1	nanoparticles					280:292	plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles	230:292	plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis	230:321	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	0	39	theme	cherry	149:154	arg1	tomatoes					156:163	cherry tomatoes	149:163	cherry tomatoes	149:163	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	3	40	theme	mechanical	659:668	arg1	resistance					670:679	high mechanical resistance	654:679	high mechanical resistance	654:679	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	1	41	theme	cinnamaldehyde	244:257	arg1	ZnONPs					295:300	ZnONPs	295:300	ZnONPs	295:300	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	1	41	theme	cinnamaldehyde	244:257	arg1	nanoparticles					280:292	plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles	230:292	plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis	230:321	Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work.					
32470591	5	42	theme	satisfactory	906:917	arg1	characterizations					930:946	satisfactory mechanical characterizations	906:946	satisfactory mechanical characterizations	906:946	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	4	43	theme	CMC-based	752:760	arg1	films					762:766	CMC-based films	752:766	CMC-based films	752:766	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	7	44	theme	fruits	1429:1434	arg1	activities					1411:1420	the metabolic activities	1397:1420	physiological the metabolic activities of the fruits	1383:1434	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	3	45	theme	low	615:617	arg1	transparency					619:630	low transparency	615:630	low transparency	615:630	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	7	46	theme	postharvest	1443:1453	arg1	period					1463:1468	postharvest storage period	1443:1468	postharvest storage period	1443:1468	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	6	47	theme	acidity	1209:1215	arg1	content					1217:1223	the total acidity content	1199:1223	the total acidity content of these fruits	1199:1239	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	7	48	theme	tomatoes	1351:1358	arg1	quality					1360:1366	the cherry tomatoes quality	1340:1366	the cherry tomatoes quality	1340:1366	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	5	49	theme	composite	832:840	arg1	film					842:845	CMC-based composite film	822:845	CMC-based composite film incorporating with both CIN and ZnONPs	822:884	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	6	50	theme	total	1203:1207	arg1	content					1217:1223	the total acidity content	1199:1223	the total acidity content of these fruits	1199:1239	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	5	51	theme	mechanical	919:928	arg1	characterizations					930:946	satisfactory mechanical characterizations	906:946	satisfactory mechanical characterizations	906:946	CMC-based composite film incorporating with both CIN and ZnONPs, in turn, exhibited satisfactory mechanical characterizations, excellent barrier capacities against water and oxygen molecules and anti-Aspergillus niger activity.					
32470591	4	52	dep	capacity	713:720	arg1	the					695:697	the	695:697	the	695:697	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	7	53	theme	physiological	1383:1395	arg1	activities					1411:1420	the metabolic activities	1397:1420	physiological the metabolic activities of the fruits	1383:1434	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	3	54	theme	high	654:657	arg1	resistance					670:679	high mechanical resistance	654:679	high mechanical resistance	654:679	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	4	55	theme	films	762:766	arg1	capacity					713:720	water barrier capacity	699:720	water barrier capacity	699:720	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	4	55	theme	films	762:766	arg1	performance					737:747	antifungal performance	726:747	antifungal performance	726:747	Furthermore, the water barrier capacity and antifungal performance of CMC-based films were significantly improved with the addition of CIN.					
32470591	0	56	theme	carboxymethylcellulose-based	64:91	arg1	coating					103:109	a carboxymethylcellulose-based composite coating	62:109	a carboxymethylcellulose-based composite coating	62:109	Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.					
32470591	7	57	theme	metabolic	1401:1409	arg1	activities					1411:1420	the metabolic activities	1397:1420	physiological the metabolic activities of the fruits	1383:1434	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	3	58	theme	good	633:636	arg1	flexibility					638:648	good flexibility	633:648	good flexibility	633:648	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	7	59	theme	cherry	1344:1349	arg1	quality					1360:1366	the cherry tomatoes quality	1340:1366	the cherry tomatoes quality	1340:1366	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32470591	6	60	theme	nanocomposite	1072:1084	arg1	film					1086:1089	ZnO/CIN/CMC nanocomposite film	1060:1089	ZnO/CIN/CMC nanocomposite film	1060:1089	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	6	61	theme	ZnO/CIN/CMC	1060:1070	arg1	film					1086:1089	ZnO/CIN/CMC nanocomposite film	1060:1089	ZnO/CIN/CMC nanocomposite film	1060:1089	Moreover, ZnO/CIN/CMC nanocomposite film was significantly effective at inhibiting the weight loss and firmness of cherry tomatoes and in decreasing the total acidity content of these fruits after storage.					
32470591	3	62	with	film	605:608	arg1	transparency					619:630	low transparency	615:630	low transparency	615:630	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	3	62	with	film	605:608	arg1	flexibility					638:648	good flexibility	633:648	good flexibility	633:648	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	3	62	with	film	605:608	arg1	resistance					670:679	high mechanical resistance	654:679	high mechanical resistance	654:679	The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance.					
32470591	2	63	theme	physico-mechanical	361:378	arg1	CMC					468:470	pristine CMC	459:470	pristine CMC	459:470	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	2	63	theme	physico-mechanical	361:378	arg1	properties					392:401	the physico-mechanical and barrier properties	357:401	properties	392:401	Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated.					
32470591	7	64	theme	ZnO/CIN/CMC	1287:1297	arg1	packaging					1318:1326	the ZnO/CIN/CMC nanocomposite film packaging	1283:1326	the ZnO/CIN/CMC nanocomposite film packaging	1283:1326	Our results suggested that the ZnO/CIN/CMC nanocomposite film packaging can improve the cherry tomatoes quality by suppressing physiological the metabolic activities of the fruits during postharvest storage period.					
32732998	0	0	theme	receptors	74:82	arg1	class					46:50	a unique class	37:50	a unique class of plant carbohydrate receptors	37:82	Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.					
32732998	7	1	theme	microbial	1076:1084	arg1	communities					1086:1096	complex microbial communities	1068:1096	complex microbial communities	1068:1096	We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.					
32732998	5	2	theme	βααβ	865:868	arg1	fold					870:873	a canonical βααβ fold	853:873	a canonical βααβ fold	853:873	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	6	3	theme	receptors	967:975	arg1	class					958:962	a ubiquitous class	945:962	a ubiquitous class of receptors in the plant kingdom	945:996	We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom.					
32732998	0	4	theme	carbohydrate	61:72	arg1	receptors					74:82	plant carbohydrate receptors	55:82	plant carbohydrate receptors	55:82	Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.					
32732998	0	5	from	signatures	11:20	arg1	EPR3					25:28	EPR3	25:28	EPR3	25:28	Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.					
32732998	5	6	contain	has	849:851	arg2	fold					870:873	a canonical βααβ fold	853:873	a canonical βααβ fold	853:873	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	5	6	contain	has	849:851	arg1	LysM3					843:847	LysM3	843:847	LysM3	843:847	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	3	7	theme	receptors	473:481	arg1	understanding					456:468	a deeper understanding	447:468	a deeper understanding of receptors involved in EPS recognition	447:509	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	5	8	contain	have	733:736	arg2	βαβ					754:756	βαβ	754:756	βαβ	754:756	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	5	8	contain	have	733:736	arg2	βαββ					745:748	unique βαββ	738:748	unique βαββ	738:748	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	5	8	contain	have	733:736	arg1	M1					723:724	M1	723:724	M1	723:724	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	5	8	contain	have	733:736	arg1	M2					730:731	M2	730:731	M2	730:731	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	3	9	theme	japonicus	553:561	arg1	ectodomain					607:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	7	10	theme	complex	1068:1074	arg1	communities					1086:1096	complex microbial communities	1068:1096	complex microbial communities	1068:1096	We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.					
32732998	5	11	located	observed	794:801	arg2	βαβ					754:756	βαβ	754:756	βαβ	754:756	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	5	11	located	observed	794:801	arg1	proteins					827:834	carbohydrate binding proteins	806:834	carbohydrate binding proteins	806:834	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	5	11	located	observed	794:801	arg2	βαββ					745:748	unique βαββ	738:748	unique βαββ	738:748	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	6	12	theme	ubiquitous	947:956	arg1	class					958:962	a ubiquitous class	945:962	a ubiquitous class of receptors in the plant kingdom	945:996	We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom.					
32732998	6	13	from	class	958:962	arg1	kingdom					990:996	the plant kingdom	980:996	the plant kingdom	980:996	We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom.					
32732998	3	14	theme	deeper	449:454	arg1	understanding					456:468	a deeper understanding	447:468	a deeper understanding of receptors involved in EPS recognition	447:509	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	3	15	theme	Lotus	547:551	arg1	ectodomain					607:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	5	16	dep	βαβ	754:756	arg1	folds					758:762	folds	758:762	folds	758:762	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	3	17	theme	exopolysaccharide	571:587	arg1	ectodomain					607:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	4	18	theme	carbohydrate-binding	674:693	arg1	M1					704:705	M1	704:705	M1	704:705	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	18	theme	carbohydrate-binding	674:693	arg1	modules					695:701	three putative carbohydrate-binding modules	659:701	three putative carbohydrate-binding modules (M1, M2 and LysM3)	659:720	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	18	theme	carbohydrate-binding	674:693	arg1	LysM3					715:719	LysM3	715:719	LysM3	715:719	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	18	theme	carbohydrate-binding	674:693	arg1	M2					708:709	M2	708:709	M2	708:709	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	0	19	theme	Structural	0:9	arg1	signatures					11:20	Structural signatures	0:20	Structural signatures in EPR3	0:28	Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.					
32732998	1	20	theme	Receptor-mediated	85:101	arg1	perception					103:112	Receptor-mediated perception	85:112	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS)	85:186	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	4	21	theme	putative	665:672	arg1	M1					704:705	M1	704:705	M1	704:705	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	21	theme	putative	665:672	arg1	modules					695:701	three putative carbohydrate-binding modules	659:701	three putative carbohydrate-binding modules (M1, M2 and LysM3)	659:720	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	21	theme	putative	665:672	arg1	LysM3					715:719	LysM3	715:719	LysM3	715:719	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	21	theme	putative	665:672	arg1	M2					708:709	M2	708:709	M2	708:709	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	7	22	theme	receptors	1119:1127	arg1	class					1110:1114	this class	1105:1114	this class of receptors	1105:1127	We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.					
32732998	3	23	theme	Lotus	564:568	arg1	ectodomain					607:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	1	24	theme	non-self	205:212	arg1	recognition					214:224	non-self recognition	205:224	non-self recognition	205:224	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	3	25	theme	receptor	589:596	arg1	ectodomain					607:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	5	26	theme	carbohydrate	806:817	arg1	proteins					827:834	carbohydrate binding proteins	806:834	carbohydrate binding proteins	806:834	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	6	27	theme	structural	920:929	arg1	signature					931:939	a structural signature	918:939	a structural signature for a ubiquitous class of receptors in the plant kingdom	918:996	We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom.					
32732998	6	27	theme	structural	920:929	arg1	configuration					901:913	this configuration	896:913	this configuration	896:913	We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom.					
32732998	1	28	from	patterns	274:281	arg1	plants					298:303	plants	298:303	plants	298:303	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	1	28	from	patterns	274:281	arg1	mammals					286:292	mammals	286:292	mammals	286:292	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	5	29	theme	binding	819:825	arg1	proteins					827:834	carbohydrate binding proteins	806:834	carbohydrate binding proteins	806:834	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	3	30	theme	EPR3	601:604	arg1	ectodomain					607:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	543:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	4	31	theme	built	650:654	arg1	structure					640:648	a compact structure	630:648	a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3)	630:720	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	2	32	theme	molecular	411:419	arg1	composition					421:431	their molecular composition	405:431	their molecular composition	405:431	In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition.					
32732998	1	33	theme	surface-exposed	117:131	arg1	carbohydrates					133:145	surface-exposed carbohydrates	117:145	surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS)	117:186	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	0	34	theme	unique	39:44	arg1	class					46:50	a unique class	37:50	a unique class of plant carbohydrate receptors	37:82	Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.					
32732998	1	35	theme	carbohydrates	133:145	arg1	perception					103:112	Receptor-mediated perception	85:112	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS)	85:186	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	6	36	theme	plant	984:988	arg1	kingdom					990:996	the plant kingdom	980:996	the plant kingdom	980:996	We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom.					
32732998	3	37	theme	EPS	495:497	arg1	recognition					499:509	EPS recognition	495:509	EPS recognition	495:509	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	4	38	dep	modules	695:701	arg1	M1					704:705	M1	704:705	M1	704:705	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	38	dep	modules	695:701	arg1	modules					695:701	three putative carbohydrate-binding modules	659:701	three putative carbohydrate-binding modules (M1, M2 and LysM3)	659:720	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	38	dep	modules	695:701	arg1	LysM3					715:719	LysM3	715:719	LysM3	715:719	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	4	38	dep	modules	695:701	arg1	M2					708:709	M2	708:709	M2	708:709	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	5	39	theme	canonical	855:863	arg1	fold					870:873	a canonical βααβ fold	853:873	a canonical βααβ fold	853:873	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	1	40	theme	microbial	243:251	arg1	patterns					274:281	microbial associated molecular patterns	243:281	microbial associated molecular patterns in mammals and plants	243:303	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	0	41	theme	plant	55:59	arg1	receptors					74:82	plant carbohydrate receptors	55:82	plant carbohydrate receptors	55:82	Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.					
32732998	2	42	with	symbiosis	368:376	arg1	rhizobia					383:390	rhizobia	383:390	rhizobia	383:390	In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition.					
32732998	3	43	theme	ectodomain	607:616	arg1	structure					530:538	the structure	526:538	the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain	526:616	To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain.					
32732998	4	44	theme	compact	632:638	arg1	structure					640:648	a compact structure	630:648	a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3)	630:720	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	1	45	theme	associated	253:262	arg1	patterns					274:281	microbial associated molecular patterns	243:281	microbial associated molecular patterns in mammals and plants	243:303	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32732998	5	46	theme	unique	738:743	arg1	βαββ					745:748	unique βαββ	738:748	unique βαββ	738:748	M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold.					
32732998	4	47	theme	modules	695:701	arg1	built					650:654	built	650:654	built	650:654	EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3).					
32732998	1	48	theme	molecular	264:272	arg1	patterns					274:281	microbial associated molecular patterns	243:281	microbial associated molecular patterns in mammals and plants	243:303	Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants.					
32409064	4	0	from	current	908:914	arg1	range					960:964	the concentration range 0.01-1.00 mM	942:977	the concentration range 0.01-1.00 mM	942:977	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	7	1	theme	sensitive	1518:1526	arg1	recognition					1528:1538	the sensitive recognition	1514:1538	the sensitive recognition of Trp enantiomers in real samples	1514:1573	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	6	2	theme	good	1379:1382	arg1	reproducibility					1384:1398	good reproducibility	1379:1398	good reproducibility	1379:1398	Moreover, the proposed NF/BPNSs-G2-β-CD/GCE showed excellent stability, good reproducibility and anti-interference capability.					
32409064	5	3	theme	hydrophobic-cavity-triggered	1217:1244	arg1	effect					1256:1261	the hydrophobic-cavity-triggered embedding effect	1213:1261	the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect	1153:1261	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	3	4	theme	wave	817:820	arg1	SWV					835:837	SWV	835:837	SWV	835:837	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	4	theme	wave	817:820	arg1	voltammetry					822:832	square wave voltammetry	810:832	square wave voltammetry (SWV)	810:838	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	2	5	theme	protective	501:510	arg1	NF					480:481	NF	480:481	NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability	480:594	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	5	theme	protective	501:510	arg1	film					512:515	a protective film	499:515	a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability	499:594	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	7	6	theme	real	1562:1565	arg1	samples					1567:1573	real samples	1562:1573	real samples	1562:1573	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	5	7	theme	embedding	1246:1254	arg1	effect					1256:1261	the hydrophobic-cavity-triggered embedding effect	1213:1261	the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect	1153:1261	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	5	8	theme	intermolecular	1157:1170	arg1	interactions					1189:1200	the intermolecular hydrogen bonding interactions	1153:1200	the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect	1153:1261	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	2	9	theme	electrode	552:560	arg1	surface					562:568	the electrode surface	548:568	the electrode surface	548:568	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	4	10	theme	Trp	923:925	arg1	enantiomers					927:937	Trp enantiomers	923:937	Trp enantiomers	923:937	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	1	11	theme	enantiomers	366:376	arg1	recognition					334:344	chiral recognition	327:344	chiral recognition of tryptophan (Trp) enantiomers	327:376	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	4	12	theme	1.07 μM	1004:1010	arg1	limits					994:999	detection limits	984:999	detection limits of 1.07 μM and 1.71 μM	984:1022	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	4	13	dep	L-Trp	1028:1032	arg1	ratio					1061:1065	signal-to-noise ratio	1045:1065	signal-to-noise ratio of 3, S/N = 3	1045:1079	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	3	14	theme	square	810:815	arg1	SWV					835:837	SWV	835:837	SWV	835:837	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	14	theme	square	810:815	arg1	voltammetry					822:832	square wave voltammetry	810:832	square wave voltammetry (SWV)	810:838	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	5	15	theme	bonding	1181:1187	arg1	interactions					1189:1200	the intermolecular hydrogen bonding interactions	1153:1200	the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect	1153:1261	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	7	16	theme	designed	1449:1456	arg1	sensor					1465:1470	the designed chiral sensor	1445:1470	the designed chiral sensor	1445:1470	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	1	17	theme	black	173:177	arg1	nanosheets					190:199	black phosphorus nanosheets	173:199	black phosphorus nanosheets (BPNSs)	173:207	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	1	17	theme	black	173:177	arg1	BPNSs					202:206	BPNSs	202:206	BPNSs	202:206	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	4	18	theme	detection	984:992	arg1	limits					994:999	detection limits	984:999	detection limits of 1.07 μM and 1.71 μM	984:1022	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	3	19	theme	peak	717:720	arg1	potential					722:730	the peak potential	713:730	the peak potential (ΔEp = ED - EL)	713:746	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	6	20	theme	excellent	1358:1366	arg1	stability					1368:1376	excellent stability	1358:1376	excellent stability	1358:1376	Moreover, the proposed NF/BPNSs-G2-β-CD/GCE showed excellent stability, good reproducibility and anti-interference capability.					
32409064	7	21	theme	chiral	1458:1463	arg1	sensor					1465:1470	the designed chiral sensor	1445:1470	the designed chiral sensor	1445:1470	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	1	22	theme	phosphorus	179:188	arg1	nanosheets					190:199	black phosphorus nanosheets	173:199	black phosphorus nanosheets (BPNSs)	173:207	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	1	22	theme	phosphorus	179:188	arg1	BPNSs					202:206	BPNSs	202:206	BPNSs	202:206	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	0	23	theme	black	18:22	arg1	phosphorus					24:33	Nafion-stabilized black phosphorus	0:33	Nafion-stabilized black phosphorus	0:33	Nafion-stabilized black phosphorus nanosheets-maltosyl-β-cyclodextrin as a chiral sensor for tryptophan enantiomers.					
32409064	4	24	theme	concentration	946:958	arg1	range					960:964	the concentration range 0.01-1.00 mM	942:977	the concentration range 0.01-1.00 mM	942:977	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	6	25	theme	anti-interference	1404:1420	arg1	capability					1422:1431	anti-interference capability	1404:1431	anti-interference capability	1404:1431	Moreover, the proposed NF/BPNSs-G2-β-CD/GCE showed excellent stability, good reproducibility and anti-interference capability.					
32409064	7	26	from	recognition	1528:1538	arg1	samples					1567:1573	real samples	1562:1573	real samples	1562:1573	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	2	27	theme	glassy	412:417	arg1	BPNSs-G2-β-CD/GCE					437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	27	theme	glassy	412:417	arg1	electrode					426:434	BPNSs-G2-β-CD composite modified glassy carbon electrode	379:434	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE)	379:454	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	0	28	theme	Nafion-stabilized	0:16	arg1	phosphorus					24:33	Nafion-stabilized black phosphorus	0:33	Nafion-stabilized black phosphorus	0:33	Nafion-stabilized black phosphorus nanosheets-maltosyl-β-cyclodextrin as a chiral sensor for tryptophan enantiomers.					
32409064	1	29	theme	novel	282:286	arg1	platform					314:321	novel electrochemical sensoring platform	282:321	novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers	282:376	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	2	30	theme	modified	403:410	arg1	BPNSs-G2-β-CD/GCE					437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	30	theme	modified	403:410	arg1	electrode					426:434	BPNSs-G2-β-CD composite modified glassy carbon electrode	379:434	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE)	379:454	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	4	31	theme	1.71 μM	1016:1022	arg1	limits					994:999	detection limits	984:999	detection limits of 1.07 μM and 1.71 μM	984:1022	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	1	32	theme	electrochemical	288:302	arg1	platform					314:321	novel electrochemical sensoring platform	282:321	novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers	282:376	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	2	33	theme	high	581:584	arg1	stability					586:594	high stability	581:594	high stability	581:594	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	4	34	theme	3	1070:1070	arg1	ratio					1061:1065	signal-to-noise ratio	1045:1065	signal-to-noise ratio of 3, S/N = 3	1045:1079	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	1	35	theme	sensoring	304:312	arg1	platform					314:321	novel electrochemical sensoring platform	282:321	novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers	282:376	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	4	36	theme	peak	903:906	arg1	current					908:914	peak current	903:914	peak current	903:914	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	5	37	theme	chiral	1287:1292	arg1	recognition					1294:1304	the effective chiral recognition	1273:1304	the effective chiral recognition	1273:1304	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	5	38	theme	chiral	1101:1106	arg1	mechanism					1120:1128	The chiral recognition mechanism	1097:1128	The chiral recognition mechanism	1097:1128	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	6	39	theme	proposed	1321:1328	arg1	NF/BPNSs-G2-β-CD/GCE					1330:1349	the proposed NF/BPNSs-G2-β-CD/GCE	1317:1349	the proposed NF/BPNSs-G2-β-CD/GCE	1317:1349	Moreover, the proposed NF/BPNSs-G2-β-CD/GCE showed excellent stability, good reproducibility and anti-interference capability.					
32409064	1	40	theme	6-O-α-maltosyl-β-cyclodextrin	213:241	arg1	BPNSs-G2-β-CD					264:276	BPNSs-G2-β-CD	264:276	BPNSs-G2-β-CD	264:276	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	1	40	theme	6-O-α-maltosyl-β-cyclodextrin	213:241	arg1	composite					253:261	6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite	213:261	6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD)	213:277	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	1	41	theme	chiral	327:332	arg1	recognition					334:344	chiral recognition	327:344	chiral recognition of tryptophan (Trp) enantiomers	327:376	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	4	42	theme	calibration	863:873	arg1	curve					875:879	a linear calibration curve	854:879	a linear calibration curve	854:879	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	3	43	theme	L-Trp	663:667	arg1	1.49					768:771	1.49	768:771	1.49	768:771	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	43	theme	L-Trp	663:667	arg1	difference					694:703	the difference	690:703	the difference between the peak potential (ΔEp = ED - EL)	690:746	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	43	theme	L-Trp	663:667	arg1	ratio					654:658	the oxidation peak current ratio	627:658	the oxidation peak current ratio of L-Trp to D-Trp (IL/ID)	627:684	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	44	theme	optimum	607:613	arg1	conditions					615:624	the optimum conditions	603:624	the optimum conditions	603:624	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	0	45	theme	chiral	75:80	arg1	sensor					82:87	a chiral sensor	73:87	a chiral sensor for tryptophan enantiomers	73:114	Nafion-stabilized black phosphorus nanosheets-maltosyl-β-cyclodextrin as a chiral sensor for tryptophan enantiomers.					
32409064	4	46	theme	linear	856:861	arg1	curve					875:879	a linear calibration curve	854:879	a linear calibration curve	854:879	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	7	47	theme	Trp	1543:1545	arg1	enantiomers					1547:1557	Trp enantiomers	1543:1557	Trp enantiomers	1543:1557	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	7	48	theme	enantiomers	1547:1557	arg1	recognition					1528:1538	the sensitive recognition	1514:1538	the sensitive recognition of Trp enantiomers in real samples	1514:1573	Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.					
32409064	4	49	theme	signal-to-noise	1045:1059	arg1	ratio					1061:1065	signal-to-noise ratio	1045:1065	signal-to-noise ratio of 3, S/N = 3	1045:1079	In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01-1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively.					
32409064	2	50	from	BPNSs-G2-β-CD	531:543	arg1	surface					562:568	the electrode surface	548:568	the electrode surface	548:568	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	3	51	theme	current	646:652	arg1	1.49					768:771	1.49	768:771	1.49	768:771	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	51	theme	current	646:652	arg1	ratio					654:658	the oxidation peak current ratio	627:658	the oxidation peak current ratio of L-Trp to D-Trp (IL/ID)	627:684	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	5	52	theme	recognition	1108:1118	arg1	mechanism					1120:1128	The chiral recognition mechanism	1097:1128	The chiral recognition mechanism	1097:1128	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	3	53	from	NF/BPNSs-G2-β-CD/GCE	786:805	arg1	difference					694:703	the difference	690:703	the difference between the peak potential (ΔEp = ED - EL)	690:746	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	53	from	NF/BPNSs-G2-β-CD/GCE	786:805	arg1	ratio					654:658	the oxidation peak current ratio	627:658	the oxidation peak current ratio of L-Trp to D-Trp (IL/ID)	627:684	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	53	from	NF/BPNSs-G2-β-CD/GCE	786:805	arg1	20 mV					777:781	20 mV	777:781	20 mV	777:781	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	53	from	NF/BPNSs-G2-β-CD/GCE	786:805	arg1	1.49					768:771	1.49	768:771	1.49	768:771	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	5	54	theme	effective	1277:1285	arg1	recognition					1294:1304	the effective chiral recognition	1273:1304	the effective chiral recognition	1273:1304	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	1	55	theme	G2-β-CD	244:250	arg1	BPNSs-G2-β-CD					264:276	BPNSs-G2-β-CD	264:276	BPNSs-G2-β-CD	264:276	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	1	55	theme	G2-β-CD	244:250	arg1	composite					253:261	6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite	213:261	6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD)	213:277	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	0	56	theme	tryptophan	93:102	arg1	enantiomers					104:114	tryptophan enantiomers	93:114	tryptophan enantiomers	93:114	Nafion-stabilized black phosphorus nanosheets-maltosyl-β-cyclodextrin as a chiral sensor for tryptophan enantiomers.					
32409064	3	57	theme	oxidation	631:639	arg1	1.49					768:771	1.49	768:771	1.49	768:771	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	57	theme	oxidation	631:639	arg1	ratio					654:658	the oxidation peak current ratio	627:658	the oxidation peak current ratio of L-Trp to D-Trp (IL/ID)	627:684	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	2	58	theme	composite	393:401	arg1	BPNSs-G2-β-CD/GCE					437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	58	theme	composite	393:401	arg1	electrode					426:434	BPNSs-G2-β-CD composite modified glassy carbon electrode	379:434	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE)	379:454	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	59	theme	carbon	419:424	arg1	BPNSs-G2-β-CD/GCE					437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	59	theme	carbon	419:424	arg1	electrode					426:434	BPNSs-G2-β-CD composite modified glassy carbon electrode	379:434	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE)	379:454	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	5	60	theme	hydrogen	1172:1179	arg1	interactions					1189:1200	the intermolecular hydrogen bonding interactions	1153:1200	the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect	1153:1261	The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition.					
32409064	1	61	theme	tryptophan	349:358	arg1	enantiomers					366:376	tryptophan (Trp) enantiomers	349:376	tryptophan (Trp) enantiomers	349:376	In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers.					
32409064	3	62	theme	peak	641:644	arg1	1.49					768:771	1.49	768:771	1.49	768:771	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	62	theme	peak	641:644	arg1	ratio					654:658	the oxidation peak current ratio	627:658	the oxidation peak current ratio of L-Trp to D-Trp (IL/ID)	627:684	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	2	63	theme	BPNSs-G2-β-CD	379:391	arg1	BPNSs-G2-β-CD/GCE					437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD/GCE	437:453	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	2	63	theme	BPNSs-G2-β-CD	379:391	arg1	electrode					426:434	BPNSs-G2-β-CD composite modified glassy carbon electrode	379:434	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE)	379:454	BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability.					
32409064	3	64	dep	potential	722:730	arg1	EL					744:745	EL	744:745	EL	744:745	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32409064	3	64	dep	potential	722:730	arg1	ΔEp = ED					733:740	ΔEp = ED	733:740	ΔEp = ED	733:740	Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (IL/ID) and the difference between the peak potential (ΔEp = ED - EL) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV).					
32545858	3	0	dep	ultraviolet	725:735	arg1	UV-Vis					738:743	UV-Vis	738:743	UV-Vis	738:743	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	3	1	theme	spectroscopic	785:797	arg1	techniques					799:808	spectroscopic techniques	785:808	spectroscopic techniques	785:808	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	8	2	theme	biological	1622:1631	arg1	environments					1633:1644	the tested biological environments	1611:1644	the tested biological environments	1611:1644	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	0	3	theme	Green	100:104	arg1	Composites					118:127	Green Synthesized Composites	100:127	Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose	100:185	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	0	4	from	Influence	0:8	arg1	Cytotoxicity					84:95	Cytotoxicity	84:95	Cytotoxicity	84:95	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	0	4	from	Influence	0:8	arg1	Activity					71:78	Antibacterial Activity	57:78	Antibacterial Activity	57:78	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	4	5	theme	minimum	857:863	arg1	concentration					876:888	minimum inhibitory concentration	857:888	minimum inhibitory concentration against Enterococcus faecalis	857:918	The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis.					
32545858	1	6	theme	antibacterial	276:288	arg1	activity					290:297	antibacterial activity	276:297	antibacterial activity	276:297	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	8	7	theme	tested	1615:1620	arg1	environments					1633:1644	the tested biological environments	1611:1644	the tested biological environments	1611:1644	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	8	8	theme	aqueous	1513:1519	arg1	dispersion					1521:1530	aqueous dispersion	1513:1530	aqueous dispersion	1513:1530	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	2	9	theme	weight	603:608	arg1	degree					561:566	different degree	551:566	different degree of substitution (DS) and molecular weight (Mw)	551:613	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	5	10	theme	human	973:977	arg1	fibroblast					988:997	human gingival fibroblast	973:997	human gingival fibroblast	973:997	The cytotoxicity of composites was assessed against human gingival fibroblast.					
32545858	0	11	theme	Composites	118:127	arg1	Cytotoxicity					84:95	Cytotoxicity	84:95	Cytotoxicity	84:95	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	0	11	theme	Composites	118:127	arg1	Activity					71:78	Antibacterial Activity	57:78	Antibacterial Activity	57:78	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	5	12	theme	gingival	979:986	arg1	fibroblast					988:997	human gingival fibroblast	973:997	human gingival fibroblast	973:997	The cytotoxicity of composites was assessed against human gingival fibroblast.					
32545858	0	13	theme	Synthesized	106:116	arg1	Composites					118:127	Green Synthesized Composites	100:127	Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose	100:185	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	3	14	dep	microscopy	675:684	arg1	techniques					799:808	spectroscopic techniques	785:808	spectroscopic techniques	785:808	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	2	15	with	CMC	542:544	arg1	degree					561:566	different degree	551:566	different degree of substitution (DS) and molecular weight (Mw)	551:613	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	8	16	theme	cytotoxicity	1593:1604	arg1	activity					1563:1570	their antibacterial activity	1543:1570	their antibacterial activity	1543:1570	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	8	16	theme	cytotoxicity	1593:1604	arg1	induction					1580:1588	the induction	1576:1588	the induction of cytotoxicity	1576:1604	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	2	17	theme	silver	478:483	arg1	ions					485:488	silver ions	478:488	silver ions	478:488	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	8	18	theme	antibacterial	1549:1561	arg1	activity					1563:1570	their antibacterial activity	1543:1570	their antibacterial activity	1543:1570	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	4	19	theme	antibacterial	815:827	arg1	activity					829:836	The antibacterial activity	811:836	The antibacterial activity	811:836	The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis.					
32545858	5	20	theme	composites	941:950	arg1	cytotoxicity					925:936	The cytotoxicity	921:936	The cytotoxicity of composites	921:950	The cytotoxicity of composites was assessed against human gingival fibroblast.					
32545858	7	21	theme	polysaccharide	1312:1325	arg1	solutions					1295:1303	aqueous solutions	1287:1303	aqueous solutions of the polysaccharide	1287:1325	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	6	22	theme	composites	1204:1213	arg1	preparation					1215:1225	composites preparation	1204:1225	composites preparation	1204:1225	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	0	23	dep	Activity	71:78	arg1	the					53:55	the	53:55	the	53:55	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	6	24	theme	AgNPs	1081:1085	arg1	morphology					1067:1076	morphology	1067:1076	morphology	1067:1076	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	6	24	theme	AgNPs	1081:1085	arg1	distribution					1050:1061	particle size distribution	1036:1061	particle size distribution	1036:1061	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	7	25	theme	CMC	1405:1407	arg1	moieties					1393:1400	AgNPs and COO- moieties	1378:1400	moieties	1393:1400	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	2	26	theme	substitution	571:582	arg1	degree					561:566	different degree	551:566	different degree of substitution (DS) and molecular weight (Mw)	551:613	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	3	27	theme	transmission	653:664	arg1	TEM					687:689	TEM	687:689	TEM	687:689	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	3	27	theme	transmission	653:664	arg1	microscopy					675:684	transmission electron microscopy	653:684	transmission electron microscopy (TEM)	653:690	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	1	28	theme	composites	319:328	arg1	cytotoxicity					303:314	cytotoxicity	303:314	cytotoxicity	303:314	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	1	28	theme	composites	319:328	arg1	activity					290:297	antibacterial activity	276:297	antibacterial activity	276:297	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	3	29	theme	electron	666:673	arg1	TEM					687:689	TEM	687:689	TEM	687:689	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	3	29	theme	electron	666:673	arg1	microscopy					675:684	transmission electron microscopy	653:684	transmission electron microscopy (TEM)	653:690	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	7	30	theme	AgNPs	1378:1382	arg1	moieties					1393:1400	AgNPs and COO- moieties	1378:1400	moieties	1393:1400	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	2	31	theme	aqueous	495:501	arg1	solutions					503:511	aqueous solutions	495:511	aqueous solutions of the polysaccharide	495:533	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	3	32	dep	infrared	704:711	arg1	ATR-FTIR					714:721	ATR-FTIR	714:721	ATR-FTIR	714:721	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	0	33	theme	Molecular	30:38	arg1	Structure					40:48	Polysaccharides' Molecular Structure	13:48	Polysaccharides' Molecular Structure	13:48	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	6	34	theme	particle	1036:1043	arg1	distribution					1050:1061	particle size distribution	1036:1061	particle size distribution	1036:1061	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	1	35	from	influence	219:227	arg1	cytotoxicity					303:314	cytotoxicity	303:314	cytotoxicity	303:314	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	1	35	from	influence	219:227	arg1	activity					290:297	antibacterial activity	276:297	antibacterial activity	276:297	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	7	36	theme	aqueous	1287:1293	arg1	solutions					1295:1303	aqueous solutions	1287:1303	aqueous solutions of the polysaccharide	1287:1325	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	2	37	theme	different	551:559	arg1	degree					561:566	different degree	551:566	different degree of substitution (DS) and molecular weight (Mw)	551:613	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	2	38	theme	molecular	593:601	arg1	Mw					611:612	Mw	611:612	Mw	611:612	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	2	38	theme	molecular	593:601	arg1	weight					603:608	molecular weight	593:608	molecular weight (Mw)	593:613	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	0	39	theme	Antibacterial	57:69	arg1	Activity					71:78	Antibacterial Activity	57:78	Antibacterial Activity	57:78	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	7	40	theme	bonds	1366:1370	arg1	formation					1335:1343	the formation	1331:1343	the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC	1331:1407	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	7	40	theme	bonds	1366:1370	arg1	dispersion					1251:1260	the dispersion	1247:1260	the dispersion of silver precursor into aqueous solutions of the polysaccharide	1247:1325	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	7	41	theme	silver	1265:1270	arg1	precursor					1272:1280	silver precursor	1265:1280	silver precursor into aqueous solutions of the polysaccharide	1265:1325	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	0	42	theme	Silver	138:143	arg1	Nanoparticles					145:157	Silver Nanoparticles	138:157	Silver Nanoparticles	138:157	Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose.					
32545858	1	43	theme	silver	339:344	arg1	AgNPs					361:365	AgNPs	361:365	AgNPs	361:365	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	1	43	theme	silver	339:344	arg1	nanoparticles					346:358	silver nanoparticles	339:358	silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC)	339:413	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	4	44	theme	inhibitory	865:874	arg1	concentration					876:888	minimum inhibitory concentration	857:888	minimum inhibitory concentration against Enterococcus faecalis	857:918	The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis.					
32545858	2	45	theme	ions	485:488	arg1	reduction					465:473	the reduction	461:473	the reduction of silver ions	461:488	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	2	46	dep	synthesized	444:454	arg1	green					438:442	green	438:442	green	438:442	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	7	47	theme	precursor	1272:1280	arg1	formation					1335:1343	the formation	1331:1343	the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC	1331:1407	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	7	47	theme	precursor	1272:1280	arg1	dispersion					1251:1260	the dispersion	1247:1260	the dispersion of silver precursor into aqueous solutions of the polysaccharide	1247:1325	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	8	48	theme	coordination	1426:1437	arg1	bonds					1439:1443	these coordination bonds	1420:1443	these coordination bonds	1420:1443	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	4	49	theme	Enterococcus	898:909	arg1	faecalis					911:918	Enterococcus faecalis	898:918	Enterococcus faecalis	898:918	The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis.					
32545858	1	50	dep	activity	290:297	arg1	the					272:274	the	272:274	the	272:274	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	7	51	theme	Ag-O	1348:1351	arg1	bonds					1366:1370	Ag-O coordination bonds	1348:1370	Ag-O coordination bonds among AgNPs and COO- moieties of CMC	1348:1407	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	7	52	theme	COO-	1388:1391	arg1	moieties					1393:1400	AgNPs and COO- moieties	1378:1400	moieties	1393:1400	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	6	53	theme	Experimental	1000:1011	arg1	evidence					1013:1020	Experimental evidence	1000:1020	Experimental evidence	1000:1020	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	7	54	theme	coordination	1353:1364	arg1	bonds					1366:1370	Ag-O coordination bonds	1348:1370	Ag-O coordination bonds among AgNPs and COO- moieties of CMC	1348:1407	This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC.					
32545858	6	55	theme	size	1045:1048	arg1	distribution					1050:1061	particle size distribution	1036:1061	particle size distribution	1036:1061	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	8	56	theme	nanoparticles	1467:1479	arg1	ability					1456:1462	the ability	1452:1462	the ability of nanoparticles to produce and release Ag+ into aqueous dispersion	1452:1530	Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.					
32545858	6	57	theme	CMC	1191:1193	arg1	DS					1178:1179	DS	1178:1179	DS	1178:1179	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	6	57	theme	CMC	1191:1193	arg1	Mw					1185:1186	Mw	1185:1186	Mw	1185:1186	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	6	57	theme	CMC	1191:1193	arg1	reaction					1153:1160	the reaction	1149:1160	the reaction	1149:1160	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	6	58	dep	DS	1178:1179	arg1	the					1174:1176	the	1174:1176	the	1174:1176	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	6	59	theme	precursor	1130:1138	arg1	quantity					1111:1118	the quantity	1107:1118	the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation	1107:1225	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	1	60	theme	molecular	249:257	arg1	structure					259:267	polysaccharides' molecular structure	232:267	polysaccharides' molecular structure	232:267	In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC).					
32545858	2	61	theme	polysaccharide	520:533	arg1	solutions					503:511	aqueous solutions	495:511	aqueous solutions of the polysaccharide	495:533	These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw).					
32545858	6	62	theme	silver	1123:1128	arg1	precursor					1130:1138	silver precursor	1123:1138	silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation	1123:1225	Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation.					
32545858	3	63	theme	X-ray	758:762	arg1	photo-electron					764:777	X-ray photo-electron	758:777	X-ray photo-electron (XPS)	758:783	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32545858	3	63	theme	X-ray	758:762	arg1	XPS					780:782	XPS	780:782	XPS	780:782	The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques.					
32994018	7	0	theme	MSC-seeded	1467:1476	arg1	scaffolds					1493:1501	MSC-seeded PHB/HA/ALG/MSC scaffolds	1467:1501	MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1467:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	1	from	regeneration	541:552	arg1	rats					614:617	rats	614:617	rats	614:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	6	2	theme	marker	1273:1278	arg1	expression					1240:1249	expression	1240:1249	expression of the CD45 phenotype marker	1240:1278	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	6	2	theme	marker	1273:1278	arg1	activity					1227:1234	ALP activity	1223:1234	ALP activity	1223:1234	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	2	3	theme	biodegradable	361:373	arg1	poly					402:405	poly	402:405	poly(3-hydroxybutyrate) (PHB)	402:430	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	3	theme	biodegradable	361:373	arg1	polymer					393:399	a biodegradable and biocompatible polymer	359:399	a biodegradable and biocompatible polymer	359:399	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	4	theme	radial	576:581	arg1	defect					583:588	the critical-sized radial defect	557:588	the critical-sized radial defect of the parietal bone in rats	557:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	7	5	theme	defect	1418:1423	arg1	%					1347:1347	94%	1345:1347	94%	1345:1347	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	5	theme	defect	1418:1423	arg1	%					1355:1355	92%	1353:1355	92%	1353:1355	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	5	theme	defect	1418:1423	arg1	defect					1418:1423	critical-sized calvarial bone defect	1388:1423	critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1388:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	5	theme	defect	1418:1423	arg1	regeneration					1372:1383	regeneration	1372:1383	regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1372:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	6	6	theme	CD45	1258:1261	arg1	marker					1273:1278	the CD45 phenotype marker	1254:1278	the CD45 phenotype marker	1254:1278	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	2	7	theme	osteogenic	294:303	arg1	efficiency					305:314	the osteogenic efficiency	290:314	the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	290:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	7	8	theme	calvarial	1403:1411	arg1	defect					1418:1423	critical-sized calvarial bone defect	1388:1423	critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1388:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	8	9	theme	in	1893:1894	arg1	properties					1913:1922	in vitro osteogenic properties	1893:1922	in vitro osteogenic properties of PHB/HA base-scaffolds	1893:1947	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	3	10	with	PHB	643:645	arg1	shapes					671:676	desired shapes	663:676	desired shapes	663:676	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32994018	7	11	from	vivo	1428:1431	arg1	%					1347:1347	94%	1345:1347	94%	1345:1347	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	11	from	vivo	1428:1431	arg1	%					1355:1355	92%	1353:1355	92%	1353:1355	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	11	from	vivo	1428:1431	arg1	defect					1418:1423	critical-sized calvarial bone defect	1388:1423	critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1388:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	11	from	vivo	1428:1431	arg1	regeneration					1372:1383	regeneration	1372:1383	regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1372:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	12	theme	parietal	597:604	arg1	bone					606:609	the parietal bone	593:609	the parietal bone	593:609	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	7	13	theme	living	1778:1783	arg1	rats					1799:1802	living postoperative rats	1778:1802	living postoperative rats	1778:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	6	14	theme	regular	1161:1167	arg1	medium					1169:1174	a regular medium	1159:1174	a regular medium	1159:1174	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	8	15	theme	osteogenic	1902:1911	arg1	properties					1913:1922	in vitro osteogenic properties	1893:1922	in vitro osteogenic properties of PHB/HA base-scaffolds	1893:1947	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	2	16	theme	scaffolds	326:334	arg1	efficiency					305:314	the osteogenic efficiency	290:314	the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	290:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	4	17	theme	acellular	896:904	arg1	PHB/ALG					906:912	acellular PHB/ALG	896:912	acellular PHB/ALG	896:912	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	7	18	theme	dyes	1770:1773	arg1	administration					1740:1753	intraperitoneal administration	1724:1753	intraperitoneal administration of fluorescent dyes to living postoperative rats	1724:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	3	19	theme	salt	705:708	arg1	technique					719:727	two-stage salt leaching technique	695:727	two-stage salt leaching technique using a mold obtained by three-dimensional printing	695:779	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32994018	0	20	theme	bone	136:139	arg1	defect					141:146	critical-sized bone defect	121:146	critical-sized bone defect	121:146	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect.					
32994018	5	21	theme	pore	1058:1061	arg1	structure					1063:1071	high porosity and irregular interconnected pore structure	1015:1071	high porosity and irregular interconnected pore structure	1015:1071	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	9	22	theme	obtained	1960:1967	arg1	data					1969:1972	the obtained data	1956:1972	the obtained data	1956:1972	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	8	23	theme	base-scaffolds	1934:1947	arg1	microstructure					1874:1887	the MSC-friendly microstructure	1857:1887	the MSC-friendly microstructure	1857:1887	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	8	23	theme	base-scaffolds	1934:1947	arg1	properties					1913:1922	in vitro osteogenic properties	1893:1922	in vitro osteogenic properties of PHB/HA base-scaffolds	1893:1947	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	7	24	theme	acellular	1602:1610	arg1	scaffolds					1623:1631	acellular PHB/HA/ALG scaffolds	1602:1631	acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1602:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	4	25	theme	hydrogel	870:877	arg1	MSCs					890:893	ALG hydrogel containing MSCs	866:893	ALG hydrogel containing MSCs	866:893	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	5	26	theme	porosity	1020:1027	arg1	structure					1063:1071	high porosity and irregular interconnected pore structure	1015:1071	high porosity and irregular interconnected pore structure	1015:1071	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	7	27	theme	administration	1740:1753	arg1	technique					1711:1719	the original technique	1698:1719	the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1698:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	6	28	from	increase	1211:1218	arg1	expression					1240:1249	expression	1240:1249	expression of the CD45 phenotype marker	1240:1278	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	6	28	from	increase	1211:1218	arg1	activity					1227:1234	ALP activity	1223:1234	ALP activity	1223:1234	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	5	29	theme	irregular	1033:1041	arg1	structure					1063:1071	high porosity and irregular interconnected pore structure	1015:1071	high porosity and irregular interconnected pore structure	1015:1071	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	7	30	from	%	1355:1355	arg1	vivo					1428:1431	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	9	31	theme	MSCs	2003:2006	arg1	potential					1990:1998	the potential	1986:1998	the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects	1986:2145	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	3	32	theme	three-dimensional	754:770	arg1	printing					772:779	three-dimensional printing	754:779	three-dimensional printing	754:779	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32994018	0	33	theme	mesenchymal	70:80	arg1	cells					87:91	mesenchymal stem cells	70:91	mesenchymal stem cells	70:91	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect.					
32994018	1	34	theme	calvarial	166:174	arg1	defect					176:181	A critical-sized calvarial defect	149:181	A critical-sized calvarial defect in rats	149:189	A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials.					
32994018	7	35	theme	original	1702:1709	arg1	technique					1711:1719	the original technique	1698:1719	the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1698:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	36	theme	tissue	1562:1567	arg1	amount					1547:1552	the main amount	1538:1552	the main amount of bone tissue	1538:1567	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	36	theme	tissue	1562:1567	arg1	tissue					1562:1567	bone tissue	1557:1567	bone tissue	1557:1567	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	37	theme	ALG	479:481	arg1	hydrogel					484:491	alginate (ALG) hydrogel	469:491	alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	469:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	1	38	from	defect	176:181	arg1	rats					186:189	rats	186:189	rats	186:189	A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials.					
32994018	7	39	from	defect	1418:1423	arg1	vivo					1428:1431	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	39	from	defect	1418:1423	arg1	day					1441:1443	28th day	1436:1443	28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1436:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	9	40	theme	bioactive	2028:2036	arg1	scaffold					2066:2073	the bioactive biopolymer/mineral/hydrogel scaffold	2024:2073	the bioactive biopolymer/mineral/hydrogel scaffold	2024:2073	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	7	41	theme	fluorescent	1669:1679	arg1	microscopy					1681:1690	fluorescent microscopy	1669:1690	fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1669:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	42	theme	main	1542:1545	arg1	amount					1547:1552	the main amount	1538:1552	the main amount of bone tissue	1538:1567	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	42	theme	main	1542:1545	arg1	tissue					1562:1567	bone tissue	1557:1567	bone tissue	1557:1567	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	43	theme	mesenchymal	504:514	arg1	MSCs					528:531	MSCs	528:531	MSCs	528:531	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	43	theme	mesenchymal	504:514	arg1	cells					521:525	mesenchymal stem cells	504:525	mesenchymal stem cells (MSCs)	504:532	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	9	44	from	process	2108:2114	arg1	defects					2139:2145	critical-sized bone defects	2119:2145	critical-sized bone defects	2119:2145	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	7	45	from	%	1347:1347	arg1	vivo					1428:1431	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	46	theme	studies	1330:1336	arg1	data					1285:1288	The data	1281:1288	The data of computed tomography and histological studies	1281:1336	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	9	47	theme	bone	2090:2093	arg1	process					2108:2114	the bone regeneration process	2086:2114	the bone regeneration process in critical-sized bone defects	2086:2145	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	6	48	from	differentiation	1140:1154	arg1	medium					1169:1174	a regular medium	1159:1174	a regular medium	1159:1174	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	2	49	with	composites	345:354	arg1	HA					453:454	HA	453:454	HA	453:454	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	49	with	composites	345:354	arg1	hydroxyapatite					437:450	hydroxyapatite	437:450	hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	437:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	7	50	theme	computed	1293:1300	arg1	tomography					1302:1311	computed tomography	1293:1311	computed tomography	1293:1311	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	51	theme	alginate	469:476	arg1	hydrogel					484:491	alginate (ALG) hydrogel	469:491	alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	469:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	52	from	defect	583:588	arg1	rats					614:617	rats	614:617	rats	614:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	4	53	theme	PHB/HA/ALG/MSC	792:805	arg1	scaffolds					807:815	PHB/HA/ALG/MSC scaffolds	792:815	PHB/HA/ALG/MSC scaffolds seeded with MSCs	792:832	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	0	54	theme	/hydroxyapatite/alginate	23:46	arg1	scaffolds					48:56	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds	0:56	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells	0:91	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect.					
32994018	9	55	theme	critical-sized	2119:2132	arg1	defects					2139:2145	critical-sized bone defects	2119:2145	critical-sized bone defects	2119:2145	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	5	56	theme	high	1015:1018	arg1	structure					1063:1071	high porosity and irregular interconnected pore structure	1015:1071	high porosity and irregular interconnected pore structure	1015:1071	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	6	57	theme	cell	1124:1127	arg1	differentiation					1140:1154	induced cell osteogenic differentiation	1116:1154	induced cell osteogenic differentiation	1116:1154	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	7	58	from	22-28 days	1572:1581	arg1	formation					1525:1533	3.6 times higher formation	1508:1533	3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1508:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	58	from	22-28 days	1572:1581	arg1	comparison					1586:1595	comparison	1586:1595	comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1586:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	59	theme	PHB/HA/ALG/MSC	1478:1491	arg1	scaffolds					1493:1501	MSC-seeded PHB/HA/ALG/MSC scaffolds	1467:1501	MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1467:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	60	theme	polymer	393:399	arg1	composites					345:354	composites	345:354	composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	345:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	60	theme	polymer	393:399	arg1	polymer					393:399	a biodegradable and biocompatible polymer	359:399	a biodegradable and biocompatible polymer	359:399	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	6	61	theme	MSC	1101:1103	arg1	growth					1105:1110	MSC growth	1101:1110	MSC growth	1101:1110	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	8	62	theme	MSC-friendly	1861:1872	arg1	microstructure					1874:1887	the MSC-friendly microstructure	1857:1887	the MSC-friendly microstructure	1857:1887	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	2	63	theme	defect	583:588	arg1	regeneration					541:552	the regeneration	537:552	the regeneration of the critical-sized radial defect of the parietal bone in rats	537:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	6	64	theme	phenotype	1263:1271	arg1	marker					1273:1278	the CD45 phenotype marker	1254:1278	the CD45 phenotype marker	1254:1278	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	7	65	theme	28th	1436:1439	arg1	day					1441:1443	28th day	1436:1443	28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1436:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	66	theme	critical-sized	561:574	arg1	defect					583:588	the critical-sized radial defect	557:588	the critical-sized radial defect of the parietal bone in rats	557:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	7	67	theme	postoperative	1785:1797	arg1	rats					1799:1802	living postoperative rats	1778:1802	living postoperative rats	1778:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	3	68	theme	two-stage	695:703	arg1	technique					719:727	two-stage salt leaching technique	695:727	two-stage salt leaching technique using a mold obtained by three-dimensional printing	695:779	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32994018	7	69	with	scaffolds	1493:1501	arg1	formation					1525:1533	3.6 times higher formation	1508:1533	3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1508:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	70	theme	bone	606:609	arg1	defect					583:588	the critical-sized radial defect	557:588	the critical-sized radial defect of the parietal bone in rats	557:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	7	71	theme	bone	1413:1416	arg1	defect					1418:1423	critical-sized calvarial bone defect	1388:1423	critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1388:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	72	theme	fluorescent	1758:1768	arg1	dyes					1770:1773	fluorescent dyes	1758:1773	fluorescent dyes	1758:1773	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	73	theme	critical-sized	1388:1401	arg1	defect					1418:1423	critical-sized calvarial bone defect	1388:1423	critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1388:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	0	74	theme	defect	141:146	arg1	regeneration					105:116	the regeneration	101:116	the regeneration of critical-sized bone defect	101:146	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect.					
32994018	2	75	theme	hybrid	319:324	arg1	scaffolds					326:334	hybrid scaffolds	319:334	hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	319:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	8	76	theme	in	1818:1819	arg1	results					1826:1832	The obtained in vivo results	1805:1832	The obtained in vivo results	1805:1832	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	7	77	from	day	1441:1443	arg1	defect					1418:1423	critical-sized calvarial bone defect	1388:1423	critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1388:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	78	theme	PHB/HA/ALG	1612:1621	arg1	scaffolds					1623:1631	acellular PHB/HA/ALG scaffolds	1602:1631	acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1602:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	8	79	dep	in	1818:1819	arg1	vivo					1821:1824	vivo	1821:1824	vivo	1821:1824	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	3	80	theme	leaching	710:717	arg1	technique					719:727	two-stage salt leaching technique	695:727	two-stage salt leaching technique using a mold obtained by three-dimensional printing	695:779	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32994018	1	81	theme	osteoinductive	217:230	arg1	properties					232:241	the osteoinductive properties	213:241	the osteoinductive properties of biomaterials	213:257	A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials.					
32994018	6	82	theme	ALP	1223:1225	arg1	activity					1227:1234	ALP activity	1223:1234	ALP activity	1223:1234	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	4	83	theme	ALG	866:868	arg1	MSCs					890:893	ALG hydrogel containing MSCs	866:893	ALG hydrogel containing MSCs	866:893	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	2	84	from	rats	614:617	arg1	regeneration					541:552	the regeneration	537:552	the regeneration of the critical-sized radial defect of the parietal bone in rats	537:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	8	85	theme	obtained	1809:1816	arg1	results					1826:1832	The obtained in vivo results	1805:1832	The obtained in vivo results	1805:1832	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	7	86	from	regeneration	1372:1383	arg1	vivo					1428:1431	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1428:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	4	87	theme	containing	879:888	arg1	MSCs					890:893	ALG hydrogel containing MSCs	866:893	ALG hydrogel containing MSCs	866:893	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	0	88	theme	stem	82:85	arg1	cells					87:91	mesenchymal stem cells	70:91	mesenchymal stem cells	70:91	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect.					
32994018	5	89	theme	interconnected	1043:1056	arg1	structure					1063:1071	high porosity and irregular interconnected pore structure	1015:1071	high porosity and irregular interconnected pore structure	1015:1071	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	1	90	theme	critical-sized	151:164	arg1	defect					176:181	A critical-sized calvarial defect	149:181	A critical-sized calvarial defect in rats	149:189	A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials.					
32994018	7	91	theme	intraperitoneal	1724:1738	arg1	administration					1740:1753	intraperitoneal administration	1724:1753	intraperitoneal administration of fluorescent dyes to living postoperative rats	1724:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	92	theme	bone	1557:1560	arg1	tissue					1562:1567	bone tissue	1557:1567	bone tissue	1557:1567	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	4	93	theme	ALG	944:946	arg1	hydrogel					948:955	empty ALG hydrogel	938:955	empty ALG hydrogel	938:955	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	7	94	theme	amount	1547:1552	arg1	formation					1525:1533	3.6 times higher formation	1508:1533	3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1508:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	8	95	dep	in	1893:1894	arg1	vitro					1896:1900	vitro	1896:1900	vitro	1896:1900	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	7	96	with	comparison	1586:1595	arg1	scaffolds					1623:1631	acellular PHB/HA/ALG scaffolds	1602:1631	acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1602:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	2	97	theme	stem	516:519	arg1	MSCs					528:531	MSCs	528:531	MSCs	528:531	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	97	theme	stem	516:519	arg1	cells					521:525	mesenchymal stem cells	504:525	mesenchymal stem cells (MSCs)	504:532	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	9	98	theme	biopolymer/mineral/hydrogel	2038:2064	arg1	scaffold					2066:2073	the bioactive biopolymer/mineral/hydrogel scaffold	2024:2073	the bioactive biopolymer/mineral/hydrogel scaffold	2024:2073	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	0	99	theme	critical-sized	121:134	arg1	defect					141:146	critical-sized bone defect	121:146	critical-sized bone defect	121:146	Poly(3-hydroxybutyrate)/hydroxyapatite/alginate scaffolds seeded with mesenchymal stem cells enhance the regeneration of critical-sized bone defect.					
32994018	7	100	theme	histological	1317:1328	arg1	studies					1330:1336	histological studies	1317:1336	histological studies	1317:1336	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	4	101	theme	empty	938:942	arg1	hydrogel					948:955	empty ALG hydrogel	938:955	empty ALG hydrogel	938:955	To obtain PHB/HA/ALG/MSC scaffolds seeded with MSCs, the scaffolds were filled with ALG hydrogel containing MSCs; acellular PHB/ALG and PHB/ALG filled with empty ALG hydrogel were prepared for comparison.					
32994018	7	102	theme	tomography	1302:1311	arg1	data					1285:1288	The data	1281:1288	The data of computed tomography and histological studies	1281:1336	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	7	103	theme	first	1655:1659	arg1	time					1661:1664	the first time	1651:1664	the first time	1651:1664	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	9	104	theme	regeneration	2095:2106	arg1	process					2108:2114	the bone regeneration process	2086:2114	the bone regeneration process in critical-sized bone defects	2086:2145	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	5	105	theme	produced	991:998	arg1	scaffolds					1000:1008	The produced scaffolds	987:1008	The produced scaffolds	987:1008	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	8	106	theme	PHB/HA	1927:1932	arg1	base-scaffolds					1934:1947	PHB/HA base-scaffolds	1927:1947	PHB/HA base-scaffolds	1927:1947	The obtained in vivo results can be associated with the MSC-friendly microstructure and in vitro osteogenic properties of PHB/HA base-scaffolds.					
32994018	6	107	theme	PHB/HA	1074:1079	arg1	scaffolds					1081:1089	PHB/HA scaffolds	1074:1089	PHB/HA scaffolds	1074:1089	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	7	108	theme	higher	1518:1523	arg1	formation					1525:1533	3.6 times higher formation	1508:1533	3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1508:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	6	109	theme	osteogenic	1129:1138	arg1	differentiation					1140:1154	induced cell osteogenic differentiation	1116:1154	induced cell osteogenic differentiation	1116:1154	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	9	110	theme	bone	2134:2137	arg1	defects					2139:2145	critical-sized bone defects	2119:2145	critical-sized bone defects	2119:2145	Thus, the obtained data demonstrate the potential of MSCs encapsulated in the bioactive biopolymer/mineral/hydrogel scaffold to improve the bone regeneration process in critical-sized bone defects.					
32994018	6	111	theme	induced	1116:1122	arg1	differentiation					1140:1154	induced cell osteogenic differentiation	1116:1154	induced cell osteogenic differentiation	1116:1154	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	5	112	contain	have	1010:1013	arg1	scaffolds					1000:1008	The produced scaffolds	987:1008	The produced scaffolds	987:1008	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	5	112	contain	have	1010:1013	arg2	structure					1063:1071	high porosity and irregular interconnected pore structure	1015:1071	high porosity and irregular interconnected pore structure	1015:1071	The produced scaffolds have high porosity and irregular interconnected pore structure.					
32994018	2	113	contain	containing	493:502	arg2	cells					521:525	mesenchymal stem cells	504:525	mesenchymal stem cells (MSCs)	504:532	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	113	contain	containing	493:502	arg1	regeneration					541:552	the regeneration	537:552	the regeneration of the critical-sized radial defect of the parietal bone in rats	537:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	113	contain	containing	493:502	arg1	hydrogel					484:491	alginate (ALG) hydrogel	469:491	alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	469:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	113	contain	containing	493:502	arg2	hydrogel					484:491	alginate (ALG) hydrogel	469:491	alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats	469:617	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	113	contain	containing	493:502	arg2	MSCs					528:531	MSCs	528:531	MSCs	528:531	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	1	114	theme	biomaterials	246:257	arg1	properties					232:241	the osteoinductive properties	213:241	the osteoinductive properties of biomaterials	213:257	A critical-sized calvarial defect in rats is employed to reveal the osteoinductive properties of biomaterials.					
32994018	7	115	theme	scaffolds	1493:1501	arg1	implantation					1451:1462	implantation	1451:1462	implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats	1451:1802	The data of computed tomography and histological studies showed 94% and 92%, respectively, regeneration of critical-sized calvarial bone defect in vivo at 28th day after implantation of MSC-seeded PHB/HA/ALG/MSC scaffolds with 3.6 times higher formation of the main amount of bone tissue at 22-28 days in comparison with acellular PHB/HA/ALG scaffolds that was shown at the first time by fluorescent microscopy using the original technique of intraperitoneal administration of fluorescent dyes to living postoperative rats.					
32994018	3	116	theme	desired	663:669	arg1	shapes					671:676	desired shapes	663:676	desired shapes	663:676	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32994018	6	117	from	growth	1105:1110	arg1	medium					1169:1174	a regular medium	1159:1174	a regular medium	1159:1174	PHB/HA scaffolds supported MSC growth and induced cell osteogenic differentiation in a regular medium in vitro that was manifested by an increase in ALP activity and expression of the CD45 phenotype marker.					
32994018	2	118	theme	biocompatible	379:391	arg1	poly					402:405	poly	402:405	poly(3-hydroxybutyrate) (PHB)	402:430	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	2	118	theme	biocompatible	379:391	arg1	polymer					393:399	a biodegradable and biocompatible polymer	359:399	a biodegradable and biocompatible polymer	359:399	In this study, we investigate the osteogenic efficiency of hybrid scaffolds based on composites of a biodegradable and biocompatible polymer, poly(3-hydroxybutyrate) (PHB) with hydroxyapatite (HA) filled with alginate (ALG) hydrogel containing mesenchymal stem cells (MSCs) on the regeneration of the critical-sized radial defect of the parietal bone in rats.					
32994018	3	119	with	PHB/HA	651:656	arg1	shapes					671:676	desired shapes	663:676	desired shapes	663:676	The scaffolds based on PHB and PHB/HA with desired shapes were prepared by two-stage salt leaching technique using a mold obtained by three-dimensional printing.					
32812835	0	0	theme	antibody-dependent	66:83	arg1	activity					98:105	the in vitro antibody-dependent phagocytosis activity	53:105	the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	53:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	6	1	theme	uptake	1254:1259	arg1	activity					1261:1268	aggregated Aβ uptake activity	1240:1268	aggregated Aβ uptake activity mediated by different effector cells	1240:1305	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	7	2	gly	afucosylated	1339:1350	arg1	N-glycans					1352:1360	afucosylated N-glycans	1339:1360	afucosylated N-glycans	1339:1360	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	1	3	theme	terminal	144:151	arg1	sugars					153:158	The terminal sugars	140:158	The terminal sugars of Fc glycans	140:172	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	1	4	theme	therapeutics	250:261	arg1	activities					216:225	the Fc-dependent biological activities	188:225	the Fc-dependent biological activities of monoclonal antibody therapeutics	188:261	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	5	5	gly	afucosylated	899:910	arg1	species					921:927	enriched afucosylated N-glycan species	890:927	enriched afucosylated N-glycan species	890:927	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	2	6	theme	cell-mediated	376:388	arg1	cytotoxicity					390:401	antibody-dependent cell-mediated cytotoxicity	357:401	antibody-dependent cell-mediated cytotoxicity (ADCC)	357:408	Afucosylated N-glycans have been shown to significantly alter binding to FcγRIIIa and affect antibody-dependent cell-mediated cytotoxicity (ADCC).					
32812835	2	6	theme	cell-mediated	376:388	arg1	ADCC					404:407	ADCC	404:407	ADCC	404:407	Afucosylated N-glycans have been shown to significantly alter binding to FcγRIIIa and affect antibody-dependent cell-mediated cytotoxicity (ADCC).					
32812835	0	7	from	Assessment	0:9	arg1	activity					98:105	the in vitro antibody-dependent phagocytosis activity	53:105	the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	53:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	6	8	theme	effector	1292:1299	arg1	cells					1301:1305	different effector cells	1282:1305	different effector cells	1282:1305	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	7	9	theme	aggregated	1458:1467	arg1	Aβ					1469:1470	aggregated Aβ	1458:1470	aggregated Aβ	1458:1470	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	7	10	theme	recombinant	1421:1431	arg1	antibody					1433:1440	a recombinant antibody	1419:1440	a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA	1419:1519	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	0	11	theme	phagocytosis	85:96	arg1	activity					98:105	the in vitro antibody-dependent phagocytosis activity	53:105	the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	53:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	7	12	theme	Fc	1481:1482	arg1	function					1493:1500	Fc effector function	1481:1500	Fc effector function	1481:1500	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	4	13	gly	afucosylated	678:689	arg1	N-glycans					691:699	afucosylated N-glycans	678:699	afucosylated N-glycans	678:699	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	6	14	theme	different	1282:1290	arg1	cells					1301:1305	different effector cells	1282:1305	different effector cells	1282:1305	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	6	15	theme	biantennary	1070:1080	arg1	glycoforms					1111:1120	high mannose afucosylated glycoforms	1085:1120	high mannose afucosylated glycoforms	1085:1120	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	6	15	theme	biantennary	1070:1080	arg1	presence					1050:1057	the presence	1046:1057	the presence of complex biantennary	1046:1080	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	6	16	theme	mannose	1090:1096	arg1	glycoforms					1111:1120	high mannose afucosylated glycoforms	1085:1120	high mannose afucosylated glycoforms	1085:1120	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	5	17	theme	afucosylated	899:910	arg1	species					921:927	enriched afucosylated N-glycan species	890:927	enriched afucosylated N-glycan species	890:927	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	5	18	theme	in	936:937	arg1	bioactivities					945:957	the in vitro bioactivities	932:957	the in vitro bioactivities	932:957	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	4	19	theme	cell	789:792	arg1	target					773:778	target	773:778	target	773:778	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	4	19	theme	cell	789:792	arg1	receptor					802:809	a cell surface receptor	787:809	a cell surface receptor	787:809	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	4	20	theme	afucosylated	678:689	arg1	N-glycans					691:699	afucosylated N-glycans	678:699	afucosylated N-glycans	678:699	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	7	21	dep	in	1387:1388	arg1	vitro					1390:1394	vitro	1390:1394	vitro	1390:1394	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	6	22	gly	fucosylated	1184:1194	arg1	glycoforms					1196:1205	fucosylated glycoforms	1184:1205	fucosylated glycoforms	1184:1205	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	7	23	theme	in	1387:1388	arg1	activity					1407:1414	the in vitro phagocytic activity	1383:1414	the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA	1383:1519	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	6	24	theme	complex	1062:1068	arg1	biantennary					1070:1080	complex biantennary	1062:1080	complex biantennary	1062:1080	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	1	25	theme	Fc	163:164	arg1	glycans					166:172	Fc glycans	163:172	Fc glycans	163:172	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	2	26	gly	Afucosylated	264:275	arg1	N-glycans					277:285	Afucosylated N-glycans	264:285	Afucosylated N-glycans	264:285	Afucosylated N-glycans have been shown to significantly alter binding to FcγRIIIa and affect antibody-dependent cell-mediated cytotoxicity (ADCC).					
32812835	6	27	theme	aggregated	1240:1249	arg1	activity					1261:1268	aggregated Aβ uptake activity	1240:1268	aggregated Aβ uptake activity mediated by different effector cells	1240:1305	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	7	28	theme	effector	1484:1491	arg1	function					1493:1500	Fc effector function	1481:1500	Fc effector function	1481:1500	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	1	29	theme	glycans	166:172	arg1	sugars					153:158	The terminal sugars	140:158	The terminal sugars of Fc glycans	140:172	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	4	30	theme	surface	794:800	arg1	target					773:778	target	773:778	target	773:778	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	4	30	theme	surface	794:800	arg1	receptor					802:809	a cell surface receptor	787:809	a cell surface receptor	787:809	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	6	31	gly	afucosylated	1098:1109	arg1	glycoforms					1111:1120	high mannose afucosylated glycoforms	1085:1120	high mannose afucosylated glycoforms	1085:1120	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	6	31	gly	afucosylated	1098:1109	arg1	biantennary					1070:1080	complex biantennary	1062:1080	complex biantennary	1062:1080	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	4	32	theme	recombinant	746:756	arg1	antibody					758:765	a recombinant antibody	744:765	a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC	744:835	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	0	33	theme	role	18:21	arg1	Assessment					0:9	Assessment	0:9	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.	0:138	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	7	34	theme	phagocytic	1396:1405	arg1	activity					1407:1414	the in vitro phagocytic activity	1383:1414	the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA	1383:1519	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	0	35	dep	in	57:58	arg1	vitro					60:64	vitro	60:64	vitro	60:64	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	6	36	theme	Aβ	1251:1252	arg1	activity					1261:1268	aggregated Aβ uptake activity	1240:1268	aggregated Aβ uptake activity mediated by different effector cells	1240:1305	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	5	37	theme	N-glycan	912:919	arg1	species					921:927	enriched afucosylated N-glycan species	890:927	enriched afucosylated N-glycan species	890:927	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	0	38	theme	antibody	113:120	arg1	activity					98:105	the in vitro antibody-dependent phagocytosis activity	53:105	the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	53:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	5	39	theme	enriched	890:897	arg1	species					921:927	enriched afucosylated N-glycan species	890:927	enriched afucosylated N-glycan species	890:927	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	7	40	theme	antibody	1433:1440	arg1	activity					1407:1414	the in vitro phagocytic activity	1383:1414	the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA	1383:1519	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	6	41	theme	binding	1155:1161	arg1	activity					1163:1170	FcγRIIIa binding activity	1146:1170	FcγRIIIa binding activity	1146:1170	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	0	42	theme	afucosylated	26:37	arg1	glycoforms					39:48	afucosylated glycoforms	26:48	afucosylated glycoforms	26:48	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	4	43	theme	biological	718:727	arg1	activities					729:738	the biological activities	714:738	the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC	714:835	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	4	44	dep	composition	652:662	arg1	the					648:650	the	648:650	the	648:650	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	1	45	theme	Fc-dependent	192:203	arg1	activities					216:225	the Fc-dependent biological activities	188:225	the Fc-dependent biological activities of monoclonal antibody therapeutics	188:261	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	6	46	theme	afucosylated	1098:1109	arg1	glycoforms					1111:1120	high mannose afucosylated glycoforms	1085:1120	high mannose afucosylated glycoforms	1085:1120	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	0	47	theme	Aβ	125:126	arg1	aggregates					128:137	Aβ aggregates	125:137	Aβ aggregates	125:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	6	48	theme	fucosylated	1184:1194	arg1	glycoforms					1196:1205	fucosylated glycoforms	1184:1205	fucosylated glycoforms	1184:1205	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	2	49	theme	Afucosylated	264:275	arg1	N-glycans					277:285	Afucosylated N-glycans	264:285	Afucosylated N-glycans	264:285	Afucosylated N-glycans have been shown to significantly alter binding to FcγRIIIa and affect antibody-dependent cell-mediated cytotoxicity (ADCC).					
32812835	1	50	theme	biological	205:214	arg1	activities					216:225	the Fc-dependent biological activities	188:225	the Fc-dependent biological activities of monoclonal antibody therapeutics	188:261	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	5	51	theme	types	862:866	arg1	impact					842:847	The impact	838:847	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities	838:957	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	5	52	from	impact	842:847	arg1	bioactivities					945:957	the in vitro bioactivities	932:957	the in vitro bioactivities	932:957	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	2	53	theme	antibody-dependent	357:374	arg1	cytotoxicity					390:401	antibody-dependent cell-mediated cytotoxicity	357:401	antibody-dependent cell-mediated cytotoxicity (ADCC)	357:408	Afucosylated N-glycans have been shown to significantly alter binding to FcγRIIIa and affect antibody-dependent cell-mediated cytotoxicity (ADCC).					
32812835	2	53	theme	antibody-dependent	357:374	arg1	ADCC					404:407	ADCC	404:407	ADCC	404:407	Afucosylated N-glycans have been shown to significantly alter binding to FcγRIIIa and affect antibody-dependent cell-mediated cytotoxicity (ADCC).					
32812835	0	54	theme	glycoforms	39:48	arg1	role					18:21	the role	14:21	the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	14:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	5	55	dep	in	936:937	arg1	vitro					939:943	vitro	939:943	vitro	939:943	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	6	56	gly	glycoforms	1111:1120	arg1	biantennary					1070:1080	complex biantennary	1062:1080	complex biantennary	1062:1080	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	6	56	gly	glycoforms	1111:1120	arg1	mannose					1090:1096	high mannose afucosylated glycoforms	1085:1120	high mannose afucosylated glycoforms	1085:1120	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	3	57	theme	appropriate	601:611	arg1	limits					613:618	appropriate limits	601:618	appropriate limits	601:618	Therefore, in order to maintain and ensure safety and efficacy for antibodies whose predominant mechanism of action (MOA) is ADCC, afucosylation is routinely monitored and controlled within appropriate limits.					
32812835	3	58	theme	action	520:525	arg1	mechanism					507:515	predominant mechanism	495:515	predominant mechanism of action (MOA)	495:531	Therefore, in order to maintain and ensure safety and efficacy for antibodies whose predominant mechanism of action (MOA) is ADCC, afucosylation is routinely monitored and controlled within appropriate limits.					
32812835	5	59	theme	amyloid	1014:1020	arg1	Aβ					1028:1029	Aβ	1028:1029	Aβ	1028:1029	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	5	59	theme	amyloid	1014:1020	arg1	beta					1022:1025	amyloid beta	1014:1025	amyloid beta (Aβ)	1014:1030	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	3	60	theme	predominant	495:505	arg1	mechanism					507:515	predominant mechanism	495:515	predominant mechanism of action (MOA)	495:531	Therefore, in order to maintain and ensure safety and efficacy for antibodies whose predominant mechanism of action (MOA) is ADCC, afucosylation is routinely monitored and controlled within appropriate limits.					
32812835	6	61	theme	FcγRIIIa	1146:1153	arg1	activity					1163:1170	FcγRIIIa binding activity	1146:1170	FcγRIIIa binding activity	1146:1170	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	5	62	theme	species	921:927	arg1	types					862:866	different types	852:866	different types	852:866	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	5	62	theme	species	921:927	arg1	levels					880:885	varying levels	872:885	varying levels of enriched afucosylated N-glycan species	872:927	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	0	63	from	activity	98:105	arg1	Assessment					0:9	Assessment	0:9	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.	0:138	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	0	64	gly	afucosylated	26:37	arg1	glycoforms					39:48	afucosylated glycoforms	26:48	afucosylated glycoforms	26:48	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	5	65	theme	levels	880:885	arg1	impact					842:847	The impact	838:847	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities	838:957	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	7	66	theme	afucosylated	1339:1350	arg1	N-glycans					1352:1360	afucosylated N-glycans	1339:1360	afucosylated N-glycans	1339:1360	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	4	67	with	case	822:825	arg1	ADCC					832:835	ADCC	832:835	ADCC	832:835	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	0	68	theme	in	57:58	arg1	activity					98:105	the in vitro antibody-dependent phagocytosis activity	53:105	the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	53:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	7	69	theme	MOA	1517:1519	arg1	part					1505:1508	part	1505:1508	part of its MOA	1505:1519	These experiments suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.					
32812835	5	70	theme	varying	872:878	arg1	levels					880:885	varying levels	872:885	varying levels of enriched afucosylated N-glycan species	872:927	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	5	71	theme	different	852:860	arg1	types					862:866	different types	852:866	different types	852:866	The impact of different types and varying levels of enriched afucosylated N-glycan species on the in vitro bioactivities is assessed for an antibody whose target is aggregated amyloid beta (Aβ).					
32812835	1	72	theme	monoclonal	230:239	arg1	therapeutics					250:261	monoclonal antibody therapeutics	230:261	monoclonal antibody therapeutics	230:261	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32812835	6	73	theme	high	1085:1088	arg1	glycoforms					1111:1120	high mannose afucosylated glycoforms	1085:1120	high mannose afucosylated glycoforms	1085:1120	While either the presence of complex biantennary or high mannose afucosylated glycoforms significantly increased FcγRIIIa binding activity compared to fucosylated glycoforms, they did not similarly increase aggregated Aβ uptake activity mediated by different effector cells.					
32812835	0	74	from	role	18:21	arg1	activity					98:105	the in vitro antibody-dependent phagocytosis activity	53:105	the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates	53:137	Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.					
32812835	4	75	theme	N-glycans	691:699	arg1	levels					668:673	levels	668:673	levels	668:673	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	4	75	theme	N-glycans	691:699	arg1	composition					652:662	composition	652:662	composition	652:662	However, it is unclear how the composition and levels of afucosylated N-glycans can modulate the biological activities for a recombinant antibody whose target is not a cell surface receptor, as is the case with ADCC.					
32812835	1	76	theme	antibody	241:248	arg1	therapeutics					250:261	monoclonal antibody therapeutics	230:261	monoclonal antibody therapeutics	230:261	The terminal sugars of Fc glycans can influence the Fc-dependent biological activities of monoclonal antibody therapeutics.					
32887306	6	0	theme	reference	927:935	arg1	method					937:942	a reference method	925:942	a reference method for the determination of milk adulteration with water	925:996	This procedure once validated on an international scale could become a reference method for the determination of milk adulteration with water.					
32887306	6	1	theme	adulteration	974:985	arg1	determination					952:964	the determination	948:964	the determination of milk adulteration with water	948:996	This procedure once validated on an international scale could become a reference method for the determination of milk adulteration with water.					
32887306	3	2	from	relation	456:463	arg1	enriched					444:451	enriched	444:451	enriched	444:451	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	1	3	theme	constituents	224:235	arg1	characteristics					139:153	the isotopic characteristics	126:153	the isotopic characteristics	126:153	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	4	4	theme	isotopic	591:598	arg1	composition					600:610	the isotopic composition	587:610	the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies	587:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	4	5	theme	δ18Owater	495:503	arg1	higher					517:522	higher	517:522	higher	517:522	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	4	5	theme	δ18Owater	495:503	arg1	values					505:510	The δ18Owater values	491:510	The δ18Owater values	491:510	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	1	6	dep	summarises	115:124	arg1	casein					245:250	casein	245:250	casein	245:250	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	1	6	dep	summarises	115:124	arg1	lactose					257:263	lactose	257:263	lactose	257:263	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	1	6	dep	summarises	115:124	arg1	water					238:242	water	238:242	water	238:242	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	4	7	theme	cow	566:568	arg1	milk					570:573	cow milk	566:573	cow milk	566:573	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	6	8	with	adulteration	974:985	arg1	water					992:996	water	992:996	water	992:996	This procedure once validated on an international scale could become a reference method for the determination of milk adulteration with water.					
32887306	4	9	theme	differences	655:665	arg1	effect					645:650	the effect	641:650	the effect of differences in the animal's thermoregulatory physiologies	641:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	4	9	theme	differences	655:665	arg1	source					630:635	water source	624:635	water source	624:635	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	3	10	theme	animal	468:473	arg1	water					484:488	animal drinking water	468:488	animal drinking water	468:488	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	4	11	theme	water	624:628	arg1	source					630:635	water source	624:635	water source	624:635	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	3	12	from	enriched	444:451	arg1	relation					456:463	relation	456:463	relation to animal drinking water	456:488	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	3	13	theme	stable	365:370	arg1	ratios					380:385	Oxygen stable isotope ratios	358:385	Oxygen stable isotope ratios in milk water	358:399	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	0	14	from	Composition	36:46	arg1	Milks					74:78	Milks	74:78	Milks	74:78	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	0	14	from	Composition	36:46	arg1	Constituents					90:101	Their Constituents	84:101	Their Constituents	84:101	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	3	15	theme	isotope	372:378	arg1	ratios					380:385	Oxygen stable isotope ratios	358:385	Oxygen stable isotope ratios in milk water	358:399	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	4	16	from	effect	645:650	arg1	physiologies					700:711	the animal's thermoregulatory physiologies	670:711	the animal's thermoregulatory physiologies	670:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	5	17	theme	water	823:827	arg1	water					823:827	added water	817:827	added water to milk	817:835	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	5	17	theme	water	823:827	arg1	amounts					800:806	lower amounts	794:806	lower amounts (>7%) of added water to milk	794:835	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	5	17	theme	water	823:827	arg1	%					811:811	>7%	809:811	>7%	809:811	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	0	18	theme	Milk	0:3	arg1	Authentication					5:18	Milk Authentication	0:18	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.	0:102	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	3	19	theme	Oxygen	358:363	arg1	ratios					380:385	Oxygen stable isotope ratios	358:385	Oxygen stable isotope ratios in milk water	358:399	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	0	20	theme	Stable	21:26	arg1	Composition					36:46	Stable Isotope Composition	21:46	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.	0:102	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	4	21	from	differences	655:665	arg1	physiologies					700:711	the animal's thermoregulatory physiologies	670:711	the animal's thermoregulatory physiologies	670:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	3	22	theme	drinking	475:482	arg1	water					484:488	animal drinking water	468:488	animal drinking water	468:488	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	3	23	theme	milk	390:393	arg1	water					395:399	milk water	390:399	milk water	390:399	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	6	24	theme	milk	969:972	arg1	adulteration					974:985	milk adulteration	969:985	milk adulteration with water	969:996	This procedure once validated on an international scale could become a reference method for the determination of milk adulteration with water.					
32887306	5	25	theme	lower	794:798	arg1	water					823:827	added water	817:827	added water to milk	817:835	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	5	25	theme	lower	794:798	arg1	amounts					800:806	lower amounts	794:806	lower amounts (>7%) of added water to milk	794:835	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	5	25	theme	lower	794:798	arg1	%					811:811	>7%	809:811	>7%	809:811	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	1	26	theme	hydrogen	173:180	arg1	isotopes					182:189	hydrogen isotopes	173:189	hydrogen isotopes	173:189	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	0	27	theme	Isotope	28:34	arg1	Composition					36:46	Stable Isotope Composition	21:46	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.	0:102	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	3	28	from	ratios	380:385	arg1	water					395:399	milk water	390:399	milk water	390:399	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	1	29	theme	Slovenian	195:203	arg1	milk					205:208	Slovenian milk	195:208	Slovenian milk	195:208	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	0	30	theme	Hydrogen	51:58	arg1	Composition					36:46	Stable Isotope Composition	21:46	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.	0:102	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	2	31	theme	isotope	297:303	arg1	ratios					305:310	the stable oxygen isotope ratios	279:310	the stable oxygen isotope ratios of cow, sheep, and goat's milk	279:341	In parallel, the stable oxygen isotope ratios of cow, sheep, and goat's milk were compared.					
32887306	1	32	dep	oxygen	162:167	arg1	i.e.					156:159	i.e.	156:159	i.e.	156:159	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	2	33	theme	oxygen	290:295	arg1	ratios					305:310	the stable oxygen isotope ratios	279:310	the stable oxygen isotope ratios of cow, sheep, and goat's milk	279:341	In parallel, the stable oxygen isotope ratios of cow, sheep, and goat's milk were compared.					
32887306	4	34	from	source	630:635	arg1	physiologies					700:711	the animal's thermoregulatory physiologies	670:711	the animal's thermoregulatory physiologies	670:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	4	35	theme	thermoregulatory	683:698	arg1	physiologies					700:711	the animal's thermoregulatory physiologies	670:711	the animal's thermoregulatory physiologies	670:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	2	36	theme	stable	283:288	arg1	ratios					305:310	the stable oxygen isotope ratios	279:310	the stable oxygen isotope ratios of cow, sheep, and goat's milk	279:341	In parallel, the stable oxygen isotope ratios of cow, sheep, and goat's milk were compared.					
32887306	1	37	theme	milk	205:208	arg1	characteristics					139:153	the isotopic characteristics	126:153	the isotopic characteristics	126:153	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	3	38	theme	seasonal	406:413	arg1	variability					415:425	seasonal variability	406:425	seasonal variability	406:425	Oxygen stable isotope ratios in milk water show seasonal variability and are also 18O enriched in relation to animal drinking water.					
32887306	5	39	theme	added	817:821	arg1	water					823:827	added water	817:827	added water to milk	817:835	The relationship between δ18Omilk and δ18Olactose is an indication that even at lower amounts (>7%) of added water to milk can be determined.					
32887306	0	40	theme	Oxygen	64:69	arg1	Composition					36:46	Stable Isotope Composition	21:46	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.	0:102	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	0	41	dep	Authentication	5:18	arg1	Composition					36:46	Stable Isotope Composition	21:46	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.	0:102	Milk Authentication: Stable Isotope Composition of Hydrogen and Oxygen in Milks and Their Constituents.					
32887306	4	42	from	physiologies	700:711	arg1	effect					645:650	the effect	641:650	the effect of differences in the animal's thermoregulatory physiologies	641:711	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	4	42	from	physiologies	700:711	arg1	source					630:635	water source	624:635	water source	624:635	The δ18Owater values were higher in sheep and goat's milk when compared to cow milk, reflecting the isotopic composition of drinking water source and the effect of differences in the animal's thermoregulatory physiologies.					
32887306	1	43	theme	isotopic	130:137	arg1	characteristics					139:153	the isotopic characteristics	126:153	the isotopic characteristics	126:153	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32887306	6	44	theme	international	892:904	arg1	scale					906:910	an international scale	889:910	an international scale	889:910	This procedure once validated on an international scale could become a reference method for the determination of milk adulteration with water.					
32887306	2	45	theme	cow	315:317	arg1	ratios					305:310	the stable oxygen isotope ratios	279:310	the stable oxygen isotope ratios of cow, sheep, and goat's milk	279:341	In parallel, the stable oxygen isotope ratios of cow, sheep, and goat's milk were compared.					
32887306	1	46	theme	major	218:222	arg1	constituents					224:235	its major constituents	214:235	its major constituents	214:235	This paper summarises the isotopic characteristics, i.e., oxygen and hydrogen isotopes, of Slovenian milk and its major constituents: water, casein, and lactose.					
32761170	4	0	theme	45 nm	589:593	arg1	distance					577:584	a distance	575:584	a distance of 45 nm between the inner and outer membranes in T. immobilis	575:647	Cryo-electron tomography data indicated a distance of 45 nm between the inner and outer membranes in T. immobilis.					
32761170	7	1	theme	multiple	1159:1166	arg1	copies					1168:1173	multiple copies	1159:1173	multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales	1159:1269	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	5	2	theme	periplasmic	675:685	arg1	space					687:691	the wide periplasmic space	666:691	the wide periplasmic space	666:691	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	9	3	theme	cell	1444:1447	arg1	envelope					1449:1456	the cell envelope	1440:1456	the cell envelope	1440:1456	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	6	4	theme	wall	921:924	arg1	proteins					926:933	two highly abundant cysteine-rich cell wall proteins	882:933	two highly abundant cysteine-rich cell wall proteins in T. immobilis	882:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	7	5	theme	cell	1132:1135	arg1	proteins					1142:1149	The cell wall proteins	1128:1149	The cell wall proteins	1128:1149	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	6	6	theme	cysteine-rich	902:914	arg1	proteins					926:933	two highly abundant cysteine-rich cell wall proteins	882:933	two highly abundant cysteine-rich cell wall proteins in T. immobilis	882:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	2	7	theme	cell	317:320	arg1	envelopes					322:330	their cell envelopes	311:330	their cell envelopes	311:330	These features raise intriguing questions about the composition of their cell envelopes.					
32761170	1	8	theme	membrane	179:186	arg1	invaginations					188:200	extensive membrane invaginations	169:200	extensive membrane invaginations	169:200	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	8	9	theme	diverging	1280:1288	arg1	taxa					1290:1293	Earlier diverging taxa	1272:1293	Earlier diverging taxa in the Planctomycetes phylum	1272:1322	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	5	10	theme	Planctomycetales	852:867	arg1	bacteria					830:837	bacteria	830:837	bacteria of the order Planctomycetales	830:867	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	10	11	theme	cellular	1655:1662	arg1	features					1664:1671	complex cellular features	1647:1671	complex cellular features	1647:1671	The results have implications for hypotheses about the process whereby complex cellular features evolve in bacteria.					
32761170	8	12	theme	Planctomycetes	1302:1315	arg1	phylum					1317:1322	the Planctomycetes phylum	1298:1322	the Planctomycetes phylum	1298:1322	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	1	13	theme	phylum	118:123	arg1	Bacteria					87:94	Bacteria	87:94	Bacteria of the Planctomycetes phylum	87:123	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	5	14	theme	bioinformatics	698:711	arg1	studies					713:719	our bioinformatics studies	694:719	our bioinformatics studies	694:719	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	5	15	from	proteins	776:783	arg1	systems					806:812	type II secretion systems	788:812	type II secretion systems	788:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	1	16	theme	many	130:133	arg1	features					151:158	many unique cellular features	130:158	many unique cellular features	130:158	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	16	theme	many	130:133	arg1	invaginations					188:200	extensive membrane invaginations	169:200	extensive membrane invaginations	169:200	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	16	theme	many	130:133	arg1	ability					210:216	the ability	206:216	the ability to import macromolecules	206:241	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	17	theme	cellular	142:149	arg1	features					151:158	many unique cellular features	130:158	many unique cellular features	130:158	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	17	theme	cellular	142:149	arg1	invaginations					188:200	extensive membrane invaginations	169:200	extensive membrane invaginations	169:200	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	17	theme	cellular	142:149	arg1	ability					210:216	the ability	206:216	the ability to import macromolecules	206:241	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	0	18	theme	Planctomycetes	64:77	arg1	Phylum					79:84	the Planctomycetes Phylum	60:84	the Planctomycetes Phylum	60:84	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum.					
32761170	9	19	theme	unique	1548:1553	arg1	phenotypes					1564:1573	other unique cellular phenotypes	1542:1573	other unique cellular phenotypes	1542:1573	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	6	20	theme	peptidoglycan	1025:1037	arg1	biosynthesis					1039:1050	peptidoglycan biosynthesis	1025:1050	peptidoglycan biosynthesis	1025:1050	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	7	21	contain	contain	1151:1157	arg1	proteins					1142:1149	The cell wall proteins	1128:1149	The cell wall proteins	1128:1149	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	7	21	contain	contain	1151:1157	arg2	copies					1168:1173	multiple copies	1159:1173	multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales	1159:1269	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	9	22	theme	phenotypes	1564:1573	arg1	emergence					1517:1525	the emergence	1513:1525	the emergence of budding and other unique cellular phenotypes	1513:1573	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	7	23	theme	only	1206:1209	arg1	domain					1211:1216	the only domain	1202:1216	the only domain that is conserved and unique to the Planctomycetales	1202:1269	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	7	23	theme	only	1206:1209	arg1	motif					1186:1190	the YTV motif	1178:1190	the YTV motif	1178:1190	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	0	24	theme	Envelope	36:43	arg1	Remodeling					13:22	Evolutionary Remodeling	0:22	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum	0:84	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum.					
32761170	4	25	from	membranes	623:631	arg1	T. immobilis					636:647	T. immobilis	636:647	T. immobilis	636:647	Cryo-electron tomography data indicated a distance of 45 nm between the inner and outer membranes in T. immobilis.					
32761170	8	26	theme	YTV	1392:1394	arg1	proteins					1406:1413	the YTV cell wall proteins	1388:1413	the YTV cell wall proteins	1388:1413	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	0	27	theme	Evolutionary	0:11	arg1	Remodeling					13:22	Evolutionary Remodeling	0:22	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum	0:84	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum.					
32761170	10	28	contain	have	1588:1591	arg2	implications					1593:1604	implications	1593:1604	implications for hypotheses about the process whereby complex cellular features evolve in bacteria	1593:1690	The results have implications for hypotheses about the process whereby complex cellular features evolve in bacteria.					
32761170	10	28	contain	have	1588:1591	arg1	results					1580:1586	The results	1576:1586	The results	1576:1586	The results have implications for hypotheses about the process whereby complex cellular features evolve in bacteria.					
32761170	8	29	theme	wall	1401:1404	arg1	proteins					1406:1413	the YTV cell wall proteins	1388:1413	the YTV cell wall proteins	1388:1413	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	3	30	theme	envelope	452:459	arg1	proteins					461:468	cell envelope proteins	447:468	cell envelope proteins	447:468	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	6	31	theme	abundant	893:900	arg1	proteins					926:933	two highly abundant cysteine-rich cell wall proteins	882:933	two highly abundant cysteine-rich cell wall proteins in T. immobilis	882:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	1	32	theme	unique	135:140	arg1	features					151:158	many unique cellular features	130:158	many unique cellular features	130:158	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	32	theme	unique	135:140	arg1	invaginations					188:200	extensive membrane invaginations	169:200	extensive membrane invaginations	169:200	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	32	theme	unique	135:140	arg1	ability					210:216	the ability	206:216	the ability to import macromolecules	206:241	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	0	33	theme	Cell	31:34	arg1	Envelope					36:43	the Cell Envelope	27:43	the Cell Envelope	27:43	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum.					
32761170	7	34	theme	YTV	1182:1184	arg1	domain					1211:1216	the only domain	1202:1216	the only domain that is conserved and unique to the Planctomycetales	1202:1269	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	7	34	theme	YTV	1182:1184	arg1	motif					1186:1190	the YTV motif	1178:1190	the YTV motif	1178:1190	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	3	35	dep	composition	418:428	arg1	the					414:416	the	414:416	the	414:416	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	5	36	with	Consistent	650:659	arg1	space					687:691	the wide periplasmic space	666:691	the wide periplasmic space	666:691	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	4	37	theme	Cryo-electron	535:547	arg1	data					560:563	Cryo-electron tomography data	535:563	Cryo-electron tomography data	535:563	Cryo-electron tomography data indicated a distance of 45 nm between the inner and outer membranes in T. immobilis.					
32761170	7	38	theme	wall	1137:1140	arg1	proteins					1142:1149	The cell wall proteins	1128:1149	The cell wall proteins	1128:1149	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	4	39	theme	inner	607:611	arg1	membranes					623:631	the inner and outer membranes	603:631	the inner and outer membranes in T. immobilis	603:647	Cryo-electron tomography data indicated a distance of 45 nm between the inner and outer membranes in T. immobilis.					
32761170	6	40	from	T. immobilis	938:949	arg1	Homologs					870:877	Homologs	870:877	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis	870:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	3	41	theme	cell	447:450	arg1	proteins					461:468	cell envelope proteins	447:468	cell envelope proteins	447:468	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	3	42	from	composition	418:428	arg1	immobilis					484:492	Tuwongella immobilis	473:492	Tuwongella immobilis	473:492	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	3	42	from	composition	418:428	arg1	members					504:510	other members	498:510	other members of the Planctomycetes	498:532	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	4	43	theme	outer	617:621	arg1	membranes					623:631	the inner and outer membranes	603:631	the inner and outer membranes in T. immobilis	603:647	Cryo-electron tomography data indicated a distance of 45 nm between the inner and outer membranes in T. immobilis.					
32761170	1	44	contain	have	125:128	arg2	ability					210:216	the ability	206:216	the ability to import macromolecules	206:241	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	44	contain	have	125:128	arg1	Bacteria					87:94	Bacteria	87:94	Bacteria of the Planctomycetes phylum	87:123	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	44	contain	have	125:128	arg2	features					151:158	many unique cellular features	130:158	many unique cellular features	130:158	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	1	44	contain	have	125:128	arg2	invaginations					188:200	extensive membrane invaginations	169:200	extensive membrane invaginations	169:200	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	3	45	from	evolution	434:442	arg1	immobilis					484:492	Tuwongella immobilis	473:492	Tuwongella immobilis	473:492	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	3	45	from	evolution	434:442	arg1	members					504:510	other members	498:510	other members of the Planctomycetes	498:532	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	3	46	theme	other	498:502	arg1	members					504:510	other members	498:510	other members of the Planctomycetes	498:532	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	6	47	theme	group	1121:1125	arg1	members					1095:1101	many members	1090:1101	many members of this bacterial group	1090:1125	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	5	48	theme	wide	670:673	arg1	space					687:691	the wide periplasmic space	666:691	the wide periplasmic space	666:691	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	6	49	theme	proteins	926:933	arg1	Homologs					870:877	Homologs	870:877	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis	870:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	6	50	from	proteins	926:933	arg1	T. immobilis					938:949	T. immobilis	938:949	T. immobilis	938:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	3	51	theme	Planctomycetes	519:532	arg1	immobilis					484:492	Tuwongella immobilis	473:492	Tuwongella immobilis	473:492	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	3	51	theme	Planctomycetes	519:532	arg1	members					504:510	other members	498:510	other members of the Planctomycetes	498:532	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	9	52	theme	envelope	1449:1456	arg1	remodeling					1426:1435	The major remodeling	1416:1435	The major remodeling of the cell envelope in the ancestor of the Planctomycetales	1416:1496	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	6	53	theme	cell	916:919	arg1	proteins					926:933	two highly abundant cysteine-rich cell wall proteins	882:933	two highly abundant cysteine-rich cell wall proteins in T. immobilis	882:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	2	54	theme	envelopes	322:330	arg1	composition					296:306	the composition	292:306	the composition of their cell envelopes	292:330	These features raise intriguing questions about the composition of their cell envelopes.					
32761170	1	55	theme	extensive	169:177	arg1	invaginations					188:200	extensive membrane invaginations	169:200	extensive membrane invaginations	169:200	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	5	56	theme	order	846:850	arg1	Planctomycetales					852:867	the order Planctomycetales	842:867	the order Planctomycetales	842:867	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	6	57	theme	Planctomycetales	989:1004	arg1	members					974:980	all members	970:980	all members of the Planctomycetales	970:1004	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	8	58	theme	Earlier	1272:1278	arg1	taxa					1290:1293	Earlier diverging taxa	1272:1293	Earlier diverging taxa in the Planctomycetes phylum	1272:1322	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	9	59	theme	Planctomycetales	1481:1496	arg1	ancestor					1465:1472	the ancestor	1461:1472	the ancestor of the Planctomycetales	1461:1496	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	6	60	from	Homologs	870:877	arg1	T. immobilis					938:949	T. immobilis	938:949	T. immobilis	938:949	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	5	61	from	segments	749:756	arg1	systems					806:812	type II secretion systems	788:812	type II secretion systems	788:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	1	62	theme	Planctomycetes	103:116	arg1	phylum					118:123	the Planctomycetes phylum	99:123	the Planctomycetes phylum	99:123	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	8	63	contain	contain	1324:1330	arg1	taxa					1290:1293	Earlier diverging taxa	1272:1293	Earlier diverging taxa in the Planctomycetes phylum	1272:1322	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	8	63	contain	contain	1324:1330	arg2	genes					1332:1336	genes	1332:1336	genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins	1332:1413	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	8	63	contain	contain	1324:1330	arg2	homologs					1376:1383	homologs	1376:1383	genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins	1332:1413	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	2	64	theme	intriguing	265:274	arg1	questions					276:284	intriguing questions	265:284	intriguing questions about the composition of their cell envelopes	265:330	These features raise intriguing questions about the composition of their cell envelopes.					
32761170	5	65	theme	proteins	776:783	arg1	segments					749:756	the periplasmic segments	733:756	the periplasmic segments of outer-membrane proteins in type II secretion systems	733:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	8	66	from	taxa	1290:1293	arg1	phylum					1317:1322	the Planctomycetes phylum	1298:1322	the Planctomycetes phylum	1298:1322	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	9	67	theme	budding	1530:1536	arg1	emergence					1517:1525	the emergence	1513:1525	the emergence of budding and other unique cellular phenotypes	1513:1573	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	6	68	theme	cell	1056:1059	arg1	elongation					1061:1070	cell elongation	1056:1070	cell elongation	1056:1070	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	0	69	theme	Phylum	79:84	arg1	Bacteria					48:55	Bacteria	48:55	Bacteria of the Planctomycetes Phylum	48:84	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum.					
32761170	5	70	theme	type	788:791	arg1	systems					806:812	type II secretion systems	788:812	type II secretion systems	788:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	9	71	theme	other	1542:1546	arg1	phenotypes					1564:1573	other unique cellular phenotypes	1542:1573	other unique cellular phenotypes	1542:1573	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	0	72	from	Remodeling	13:22	arg1	Bacteria					48:55	Bacteria	48:55	Bacteria of the Planctomycetes Phylum	48:84	Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum.					
32761170	8	73	theme	peptidoglycan	1342:1354	arg1	biosynthesis					1356:1367	peptidoglycan biosynthesis	1342:1367	peptidoglycan biosynthesis	1342:1367	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	5	74	theme	periplasmic	737:747	arg1	segments					749:756	the periplasmic segments	733:756	the periplasmic segments of outer-membrane proteins in type II secretion systems	733:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	5	75	from	systems	806:812	arg1	segments					749:756	the periplasmic segments	733:756	the periplasmic segments of outer-membrane proteins in type II secretion systems	733:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	9	76	theme	cellular	1555:1562	arg1	phenotypes					1564:1573	other unique cellular phenotypes	1542:1573	other unique cellular phenotypes	1542:1573	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	6	77	theme	bacterial	1111:1119	arg1	group					1121:1125	this bacterial group	1106:1125	this bacterial group	1106:1125	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	3	78	used	used	356:359	arg2	we					348:349	we	348:349	we	348:349	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	7	79	theme	motif	1186:1190	arg1	copies					1168:1173	multiple copies	1159:1173	multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales	1159:1269	The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales.					
32761170	6	80	theme	many	1090:1093	arg1	members					1095:1101	many members	1090:1101	many members of this bacterial group	1090:1125	Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost in many members of this bacterial group.					
32761170	8	81	theme	cell	1396:1399	arg1	proteins					1406:1413	the YTV cell wall proteins	1388:1413	the YTV cell wall proteins	1388:1413	Earlier diverging taxa in the Planctomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins.					
32761170	3	82	theme	proteins	461:468	arg1	evolution					434:442	evolution	434:442	evolution	434:442	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	3	82	theme	proteins	461:468	arg1	composition					418:428	composition	418:428	composition	418:428	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	5	83	theme	secretion	796:804	arg1	systems					806:812	type II secretion systems	788:812	type II secretion systems	788:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	9	84	from	remodeling	1426:1435	arg1	ancestor					1465:1472	the ancestor	1461:1472	the ancestor of the Planctomycetales	1461:1496	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
32761170	5	85	theme	outer-membrane	761:774	arg1	proteins					776:783	outer-membrane proteins	761:783	outer-membrane proteins in type II secretion systems	761:812	Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer-membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales.					
32761170	4	86	theme	tomography	549:558	arg1	data					560:563	Cryo-electron tomography data	535:563	Cryo-electron tomography data	535:563	Cryo-electron tomography data indicated a distance of 45 nm between the inner and outer membranes in T. immobilis.					
32761170	3	87	theme	Tuwongella	473:482	arg1	immobilis					484:492	Tuwongella immobilis	473:492	Tuwongella immobilis	473:492	In this study, we have used microscopy, phylogenomics, and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes.					
32761170	1	88	theme	import	221:226	arg1	macromolecules					228:241	import macromolecules	221:241	import macromolecules	221:241	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules.					
32761170	10	89	theme	complex	1647:1653	arg1	features					1664:1671	complex cellular features	1647:1671	complex cellular features	1647:1671	The results have implications for hypotheses about the process whereby complex cellular features evolve in bacteria.					
32761170	9	90	theme	major	1420:1424	arg1	remodeling					1426:1435	The major remodeling	1416:1435	The major remodeling of the cell envelope in the ancestor of the Planctomycetales	1416:1496	The major remodeling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes.					
33333098	2	0	theme	@	459:459	arg1	beads					470:474	easily separable TATGO@CS hybrid beads	437:474	easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	437:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	3	1	dep	EDAX	658:661	arg1	FTIR					652:655	SEM, TGA, FTIR, EDAX	642:661	FTIR	652:655	The instrumentations such as SEM, TGA, FTIR, EDAX, XRD and BET studies were executed for adsorbents.					
33333098	3	1	dep	EDAX	658:661	arg1	TGA					647:649	SEM, TGA, FTIR, EDAX	642:661	TGA	647:649	The instrumentations such as SEM, TGA, FTIR, EDAX, XRD and BET studies were executed for adsorbents.					
33333098	3	1	dep	EDAX	658:661	arg1	studies					676:682	studies	676:682	studies	676:682	The instrumentations such as SEM, TGA, FTIR, EDAX, XRD and BET studies were executed for adsorbents.					
33333098	2	2	theme	structural	344:353	arg1	property					355:362	the structural property	340:362	the structural property of TATGO composite	340:381	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	6	3	from	attraction	1118:1127	arg1	NO3-					1132:1135	NO3-	1132:1135	NO3-	1132:1135	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	6	3	from	attraction	1118:1127	arg1	PO43-					1141:1145	PO43-	1141:1145	PO43-	1141:1145	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	2	4	theme	TATGO	454:458	arg1	beads					470:474	easily separable TATGO@CS hybrid beads	437:474	easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	437:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	5	5	dep	parameters	985:994	arg1	parameters					985:994	thermodynamic parameters	971:994	thermodynamic parameters (ΔG°, ΔH° and ΔS°)	971:1013	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	5	dep	parameters	985:994	arg1	ΔS°					1010:1012	ΔS°	1010:1012	ΔS°	1010:1012	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	5	dep	parameters	985:994	arg1	ΔH°					1002:1004	ΔH°	1002:1004	ΔH°	1002:1004	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	5	dep	parameters	985:994	arg1	ΔG°					997:999	ΔG°	997:999	ΔG°	997:999	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	6	theme	adsorption	1022:1031	arg1	effect					818:823	The effect	814:823	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption	814:1031	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	1	7	theme	TATGO	254:258	arg1	composite					260:268	TATGO composite	254:268	TATGO composite	254:268	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	2	8	theme	61.38 mg/g	556:565	arg1	capacities					532:541	the significant NO3- and PO43- adsorption capacities	490:541	the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	490:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	6	9	theme	electrostatic	1104:1116	arg1	mechanism					1059:1067	The removal mechanism	1047:1067	The removal mechanism of TATGO@CS hybrid beads	1047:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	6	9	theme	electrostatic	1104:1116	arg1	attraction					1118:1127	electrostatic attraction	1104:1127	electrostatic attraction on NO3- and PO43-	1104:1145	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	6	10	theme	@	1077:1077	arg1	beads					1088:1092	TATGO@CS hybrid beads	1072:1092	TATGO@CS hybrid beads	1072:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	0	11	theme	nutrients	105:113	arg1	remediation					115:125	nutrients remediation	105:125	nutrients remediation towards water purification	105:152	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	5	12	theme	pseudo-first/second	903:921	arg1	kinetics					893:900	kinetics	893:900	kinetics (pseudo-first/second order and particle/intraparticle diffusion)	893:965	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	12	theme	pseudo-first/second	903:921	arg1	order					923:927	pseudo-first/second order	903:927	pseudo-first/second order	903:927	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	6	13	theme	removal	1051:1057	arg1	mechanism					1059:1067	The removal mechanism	1047:1067	The removal mechanism of TATGO@CS hybrid beads	1047:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	6	13	theme	removal	1051:1057	arg1	attraction					1118:1127	electrostatic attraction	1104:1127	electrostatic attraction on NO3- and PO43-	1104:1145	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	0	14	theme	water	135:139	arg1	purification					141:152	water purification	135:152	water purification	135:152	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	7	15	theme	CS	1191:1192	arg1	beads					1201:1205	TATGO@CS hybrid beads	1185:1205	TATGO@CS hybrid beads	1185:1205	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	4	16	theme	batch	801:805	arg1	scale					807:811	batch scale	801:811	batch scale	801:811	The optimization of the parameters accountable for adsorption process was performed in batch scale.					
33333098	2	17	theme	separable	444:452	arg1	beads					470:474	easily separable TATGO@CS hybrid beads	437:474	easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	437:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	2	18	theme	individual	591:600	arg1	materials					602:610	their individual materials	585:610	their individual materials	585:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	0	19	theme	composite	85:93	arg1	beads					95:99	graphene oxide capped chitosan porous composite beads	47:99	graphene oxide capped chitosan porous composite beads	47:99	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	6	20	theme	beads	1088:1092	arg1	mechanism					1059:1067	The removal mechanism	1047:1067	The removal mechanism of TATGO@CS hybrid beads	1047:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	6	20	theme	beads	1088:1092	arg1	attraction					1118:1127	electrostatic attraction	1104:1127	electrostatic attraction on NO3- and PO43-	1104:1145	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	7	21	theme	@	1190:1190	arg1	beads					1201:1205	TATGO@CS hybrid beads	1185:1205	TATGO@CS hybrid beads	1185:1205	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	2	22	theme	composite	373:381	arg1	property					355:362	the structural property	340:362	the structural property of TATGO composite	340:381	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	6	23	theme	hybrid	1081:1086	arg1	beads					1088:1092	TATGO@CS hybrid beads	1072:1092	TATGO@CS hybrid beads	1072:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	5	24	dep	kinetics	893:900	arg1	kinetics					893:900	kinetics	893:900	kinetics (pseudo-first/second order and particle/intraparticle diffusion)	893:965	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	24	dep	kinetics	893:900	arg1	order					923:927	pseudo-first/second order	903:927	pseudo-first/second order	903:927	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	24	dep	kinetics	893:900	arg1	diffusion					956:964	particle/intraparticle diffusion	933:964	particle/intraparticle diffusion	933:964	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	2	25	theme	TATGO	367:371	arg1	composite					373:381	TATGO composite	367:381	TATGO composite	367:381	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	2	26	theme	PO43-	515:519	arg1	adsorption					521:530	PO43- adsorption	515:530	PO43- adsorption	515:530	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	6	27	theme	CS	1078:1079	arg1	beads					1088:1092	TATGO@CS hybrid beads	1072:1092	TATGO@CS hybrid beads	1072:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	0	28	theme	triaminotriazine	15:30	arg1	Development					0:10	Development	0:10	Development of triaminotriazine	0:30	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	1	29	theme	nutrients	288:296	arg1	retention					315:323	nutrients (NO3- and PO43-) retention	288:323	nutrients (NO3- and PO43-) retention	288:323	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	5	30	dep	isotherms	828:836	arg1	Dubinin-Radushkevich					864:883	Dubinin-Radushkevich	864:883	Dubinin-Radushkevich (D-R)	864:889	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	30	dep	isotherms	828:836	arg1	Freundlich					849:858	Freundlich	849:858	Freundlich	849:858	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	30	dep	isotherms	828:836	arg1	isotherms					828:836	isotherms	828:836	isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R))	828:890	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	30	dep	isotherms	828:836	arg1	Langmuir					839:846	Langmuir	839:846	Langmuir	839:846	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	30	dep	isotherms	828:836	arg1	D-R					886:888	D-R	886:888	D-R	886:888	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	31	theme	kinetics	893:900	arg1	effect					818:823	The effect	814:823	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption	814:1031	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	1	32	theme	grafted	217:223	arg1	oxide					234:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide	155:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite	155:268	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	0	33	theme	oxide	56:60	arg1	chitosan					69:76	graphene oxide capped chitosan	47:76	graphene oxide capped chitosan porous composite beads	47:99	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	2	34	theme	NO3-	506:509	arg1	capacities					532:541	the significant NO3- and PO43- adsorption capacities	490:541	the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	490:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	7	35	theme	beads	1201:1205	arg1	reuse					1176:1180	reuse	1176:1180	reuse	1176:1180	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	7	35	theme	beads	1201:1205	arg1	applicability					1158:1170	field applicability	1152:1170	field applicability	1152:1170	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	5	36	theme	thermodynamic	971:983	arg1	parameters					985:994	thermodynamic parameters	971:994	thermodynamic parameters (ΔG°, ΔH° and ΔS°)	971:1013	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	36	theme	thermodynamic	971:983	arg1	ΔS°					1010:1012	ΔS°	1010:1012	ΔS°	1010:1012	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	36	theme	thermodynamic	971:983	arg1	ΔH°					1002:1004	ΔH°	1002:1004	ΔH°	1002:1004	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	36	theme	thermodynamic	971:983	arg1	ΔG°					997:999	ΔG°	997:999	ΔG°	997:999	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	37	theme	isotherms	828:836	arg1	effect					818:823	The effect	814:823	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption	814:1031	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	1	38	theme	graphene	225:232	arg1	oxide					234:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide	155:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite	155:268	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	0	39	theme	graphene	47:54	arg1	chitosan					69:76	graphene oxide capped chitosan	47:76	graphene oxide capped chitosan porous composite beads	47:99	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	2	40	theme	significant	494:504	arg1	NO3-					506:509	significant NO3-	494:509	significant NO3-	494:509	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	7	41	dep	applicability	1158:1170	arg1	The					1148:1150	The	1148:1150	The	1148:1150	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	4	42	theme	adsorption	765:774	arg1	process					776:782	adsorption process	765:782	adsorption process	765:782	The optimization of the parameters accountable for adsorption process was performed in batch scale.					
33333098	0	43	theme	chitosan	69:76	arg1	beads					95:99	graphene oxide capped chitosan porous composite beads	47:99	graphene oxide capped chitosan porous composite beads	47:99	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	2	44	theme	58.46	546:550	arg1	capacities					532:541	the significant NO3- and PO43- adsorption capacities	490:541	the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	490:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	2	45	theme	CS	460:461	arg1	beads					470:474	easily separable TATGO@CS hybrid beads	437:474	easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	437:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	6	46	theme	TATGO	1072:1076	arg1	beads					1088:1092	TATGO@CS hybrid beads	1072:1092	TATGO@CS hybrid beads	1072:1092	The removal mechanism of TATGO@CS hybrid beads was to be electrostatic attraction on NO3- and PO43-.					
33333098	0	47	theme	capped	62:67	arg1	chitosan					69:76	graphene oxide capped chitosan	47:76	graphene oxide capped chitosan porous composite beads	47:99	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	7	48	theme	hybrid	1194:1199	arg1	beads					1201:1205	TATGO@CS hybrid beads	1185:1205	TATGO@CS hybrid beads	1185:1205	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	4	49	theme	accountable	749:759	arg1	parameters					738:747	the parameters	734:747	the parameters accountable for adsorption process	734:782	The optimization of the parameters accountable for adsorption process was performed in batch scale.					
33333098	5	50	theme	particle/intraparticle	933:954	arg1	kinetics					893:900	kinetics	893:900	kinetics (pseudo-first/second order and particle/intraparticle diffusion)	893:965	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	5	50	theme	particle/intraparticle	933:954	arg1	diffusion					956:964	particle/intraparticle diffusion	933:964	particle/intraparticle diffusion	933:964	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	4	51	theme	parameters	738:747	arg1	optimization					718:729	The optimization	714:729	The optimization of the parameters accountable for adsorption process	714:782	The optimization of the parameters accountable for adsorption process was performed in batch scale.					
33333098	5	52	theme	parameters	985:994	arg1	effect					818:823	The effect	814:823	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption	814:1031	The effect of isotherms (Langmuir, Freundlich and Dubinin-Radushkevich (D-R)), kinetics (pseudo-first/second order and particle/intraparticle diffusion) and thermodynamic parameters (ΔG°, ΔH° and ΔS°) of the adsorption was explored.					
33333098	7	53	theme	TATGO	1185:1189	arg1	beads					1201:1205	TATGO@CS hybrid beads	1185:1205	TATGO@CS hybrid beads	1185:1205	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	2	54	theme	adsorption	521:530	arg1	capacities					532:541	the significant NO3- and PO43- adsorption capacities	490:541	the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	490:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	1	55	dep	oxide	234:238	arg1	GO					241:242	GO	241:242	GO	241:242	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	1	56	theme	porous	159:164	arg1	oxide					234:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide	155:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite	155:268	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	2	57	theme	hybrid	463:468	arg1	beads					470:474	easily separable TATGO@CS hybrid beads	437:474	easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	437:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	7	58	theme	field	1152:1156	arg1	applicability					1158:1170	field applicability	1152:1170	field applicability	1152:1170	The field applicability and reuse of TATGO@CS hybrid beads was also inspected.					
33333098	0	59	theme	porous	78:83	arg1	beads					95:99	graphene oxide capped chitosan porous composite beads	47:99	graphene oxide capped chitosan porous composite beads	47:99	Development of triaminotriazine functionalized graphene oxide capped chitosan porous composite beads for nutrients remediation towards water purification.					
33333098	1	60	dep	nutrients	288:296	arg1	nutrients					288:296	nutrients	288:296	nutrients (NO3- and PO43-) retention	288:323	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	1	60	dep	nutrients	288:296	arg1	PO43-					308:312	PO43-	308:312	PO43-	308:312	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	1	60	dep	nutrients	288:296	arg1	NO3-					299:302	NO3-	299:302	NO3-	299:302	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
33333098	2	61	theme	chitosan	412:419	arg1	use					405:407	the use	401:407	the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	401:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	2	62	contain	possess	482:488	arg2	capacities					532:541	the significant NO3- and PO43- adsorption capacities	490:541	the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	490:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	2	62	contain	possess	482:488	arg1	beads					470:474	easily separable TATGO@CS hybrid beads	437:474	easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials	437:610	Additionally, the structural property of TATGO composite was improved with the use of chitosan (CS) to produce easily separable TATGO@CS hybrid beads which possess the significant NO3- and PO43- adsorption capacities of 58.46 and 61.38 mg/g respectively than their individual materials.					
33333098	1	63	theme	definite	167:174	arg1	oxide					234:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide	155:238	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite	155:268	The porous, definite and nitrogen rich triaminotriazine (TAT) grafted graphene oxide (GO) known as TATGO composite was developed for nutrients (NO3- and PO43-) retention.					
32480237	0	0	theme	calcium	91:97	arg1	competition					63:73	the competition	59:73	the competition of aluminum and calcium for pectin binding	59:116	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	2	1	theme	African	442:448	arg1	grass					450:454	an African grass	439:454	an African grass highly adapted to acidic Al-rich soils	439:493	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	2	1	theme	African	442:448	arg1	decumbens					428:436	Urochloa decumbens	419:436	Urochloa decumbens	419:436	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	1	2	theme	cell	297:300	arg1	composition					307:317	cell wall composition	297:317	cell wall composition	297:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	3	theme	wall	302:305	arg1	composition					307:317	cell wall composition	297:317	cell wall composition	297:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	3	4	from	Variations	496:505	arg1	composition					514:524	composition	514:524	composition	514:524	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	4	from	Variations	496:505	arg1	distribution					530:541	distribution	530:541	distribution	530:541	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	0	5	theme	pectin	103:108	arg1	binding					110:116	pectin binding	103:116	pectin binding	103:116	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	3	6	theme	walls	642:646	arg1	extensibility					569:581	the extensibility	565:581	the extensibility	565:581	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	6	theme	walls	642:646	arg1	porosity					594:601	porosity	594:601	porosity	594:601	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	6	theme	walls	642:646	arg1	properties					617:626	adhesive properties	608:626	adhesive properties	608:626	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	6	theme	walls	642:646	arg1	rigidity					584:591	rigidity	584:591	rigidity	584:591	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	7	dep	160	741:743	arg1	to					731:732	to	731:732	to	731:732	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	8	theme	Al3+	759:762	arg1	pH					707:708	pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM	707:754	pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h	707:770	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	2	9	theme	acidic	474:479	arg1	soils					489:493	acidic Al-rich soils	474:493	acidic Al-rich soils	474:493	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	4	10	theme	soil	836:839	arg1	acidity					841:847	soil acidity	836:847	soil acidity	836:847	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	6	11	theme	DFT	1120:1122	arg1	analyses					1125:1132	Density Functional Theory (DFT) analyses	1093:1132	Density Functional Theory (DFT) analyses	1093:1132	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	6	12	theme	complexes	1239:1247	arg1	extension					1345:1353	hampered cell extension	1331:1353	hampered cell extension	1331:1353	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	6	12	theme	complexes	1239:1247	arg1	formation					1208:1216	the formation	1204:1216	the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls,	1204:1325	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	0	13	from	evidence	50:57	arg1	roots					140:144	Urochloa decumbens roots	121:144	Urochloa decumbens roots	121:144	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	5	14	from	variations	949:958	arg1	composition					970:980	pectin composition	963:980	pectin composition induced both by Al3+ and by H+ in root tissues and zones	963:1037	Immunocytochemical approaches demonstrated variations in pectin composition induced both by Al3+ and by H+ in root tissues and zones.					
32480237	6	15	theme	Theory	1112:1117	arg1	analyses					1125:1132	Density Functional Theory (DFT) analyses	1093:1132	Density Functional Theory (DFT) analyses	1093:1132	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	6	16	theme	Al3+-pectate	1226:1237	arg1	complexes					1239:1247	more Al3+-pectate complexes	1221:1247	more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls,	1221:1325	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	3	17	theme	cell	637:640	arg1	walls					642:646	plant cell walls	631:646	plant cell walls	631:646	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	18	theme	decumbens	686:694	arg1	seedlings					670:678	seedlings	670:678	seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h	670:770	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	6	19	theme	Functional	1101:1110	arg1	analyses					1125:1132	Density Functional Theory (DFT) analyses	1093:1132	Density Functional Theory (DFT) analyses	1093:1132	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	1	20	theme	low	184:186	arg1	consequence					249:259	a consequence	247:259	a consequence of the interaction between Al3+ and cell wall composition	247:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	20	theme	low	184:186	arg1	[Al3+					226:230	abundant soluble aluminum [Al3+]	200:231	abundant soluble aluminum [Al3+]	200:231	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	20	theme	low	184:186	arg1	pH					188:189	low pH	184:189	low pH [H+]	184:194	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	0	21	theme	Functional	32:41	arg1	Theory					43:48	Density Functional Theory	24:48	Density Functional Theory	24:48	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	4	22	from	pH	898:899	arg1	reduction					865:873	effective reduction	855:873	effective reduction of root growth only at pH 3.5	855:903	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	0	23	theme	decumbens	130:138	arg1	roots					140:144	Urochloa decumbens roots	121:144	Urochloa decumbens roots	121:144	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	2	24	theme	Al-rich	481:487	arg1	soils					489:493	acidic Al-rich soils	474:493	acidic Al-rich soils	474:493	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	0	25	theme	Density	24:30	arg1	Theory					43:48	Density Functional Theory	24:48	Density Functional Theory	24:48	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	0	26	theme	Urochloa	121:128	arg1	roots					140:144	Urochloa decumbens roots	121:144	Urochloa decumbens roots	121:144	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	2	27	theme	pectin	376:381	arg1	molecules					383:391	pectin molecules	376:391	pectin molecules	376:391	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	3	28	theme	U.	683:684	arg1	decumbens					686:694	U. decumbens	683:694	U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h	683:770	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	6	29	theme	Density	1093:1099	arg1	analyses					1125:1132	Density Functional Theory (DFT) analyses	1093:1132	Density Functional Theory (DFT) analyses	1093:1132	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	6	30	theme	cell	1340:1343	arg1	extension					1345:1353	hampered cell extension	1331:1353	hampered cell extension	1331:1353	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	2	31	theme	decumbens	428:436	arg1	roots					410:414	roots	410:414	roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils	410:493	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	5	32	theme	root	1016:1019	arg1	tissues					1021:1027	root tissues	1016:1027	root tissues	1016:1027	Immunocytochemical approaches demonstrated variations in pectin composition induced both by Al3+ and by H+ in root tissues and zones.					
32480237	6	33	theme	Ca2+-pectate	1254:1265	arg1	complexes					1267:1275	Ca2+-pectate complexes	1254:1275	Ca2+-pectate complexes	1254:1275	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	5	34	theme	pectin	963:968	arg1	composition					970:980	pectin composition	963:980	pectin composition induced both by Al3+ and by H+ in root tissues and zones	963:1037	Immunocytochemical approaches demonstrated variations in pectin composition induced both by Al3+ and by H+ in root tissues and zones.					
32480237	2	35	theme	Urochloa	419:426	arg1	grass					450:454	an African grass	439:454	an African grass highly adapted to acidic Al-rich soils	439:493	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	2	35	theme	Urochloa	419:426	arg1	decumbens					428:436	Urochloa decumbens	419:436	Urochloa decumbens	419:436	The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils.					
32480237	1	36	theme	interaction	268:278	arg1	consequence					249:259	a consequence	247:259	a consequence of the interaction between Al3+ and cell wall composition	247:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	36	theme	interaction	268:278	arg1	[Al3+					226:230	abundant soluble aluminum [Al3+]	200:231	abundant soluble aluminum [Al3+]	200:231	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	36	theme	interaction	268:278	arg1	pH					188:189	low pH	184:189	low pH [H+]	184:194	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	4	37	theme	Root	773:776	arg1	growth					778:783	Root growth	773:783	Root growth	773:783	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	5	38	theme	Immunocytochemical	906:923	arg1	approaches					925:934	Immunocytochemical approaches	906:934	Immunocytochemical approaches	906:934	Immunocytochemical approaches demonstrated variations in pectin composition induced both by Al3+ and by H+ in root tissues and zones.					
32480237	4	39	theme	root	878:881	arg1	growth					883:888	root growth	878:888	root growth	878:888	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	3	40	theme	pectins	546:552	arg1	composition					514:524	composition	514:524	composition	514:524	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	3	40	theme	pectins	546:552	arg1	distribution					530:541	distribution	530:541	distribution	530:541	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	6	41	theme	hampered	1331:1338	arg1	extension					1345:1353	hampered cell extension	1331:1353	hampered cell extension	1331:1353	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	3	42	theme	plant	631:635	arg1	walls					642:646	plant cell walls	631:646	plant cell walls	631:646	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	1	43	theme	abundant	200:207	arg1	consequence					249:259	a consequence	247:259	a consequence of the interaction between Al3+ and cell wall composition	247:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	43	theme	abundant	200:207	arg1	[Al3+					226:230	abundant soluble aluminum [Al3+]	200:231	abundant soluble aluminum [Al3+]	200:231	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	43	theme	abundant	200:207	arg1	pH					188:189	low pH	184:189	low pH [H+]	184:194	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	3	44	theme	adhesive	608:615	arg1	properties					617:626	adhesive properties	608:626	adhesive properties	608:626	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	1	45	theme	soluble	209:215	arg1	consequence					249:259	a consequence	247:259	a consequence of the interaction between Al3+ and cell wall composition	247:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	45	theme	soluble	209:215	arg1	[Al3+					226:230	abundant soluble aluminum [Al3+]	200:231	abundant soluble aluminum [Al3+]	200:231	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	45	theme	soluble	209:215	arg1	pH					188:189	low pH	184:189	low pH [H+]	184:194	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	0	46	theme	aluminum	78:85	arg1	competition					63:73	the competition	59:73	the competition of aluminum and calcium for pectin binding	59:116	Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots.					
32480237	6	47	theme	higher	1296:1301	arg1	rigidity					1303:1310	higher rigidity	1296:1310	higher rigidity of cell walls	1296:1324	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	1	48	theme	Root	147:150	arg1	growth					152:157	Root growth	147:157	Root growth	147:157	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	6	49	theme	walls	1320:1324	arg1	rigidity					1303:1310	higher rigidity	1296:1310	higher rigidity of cell walls	1296:1324	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	1	50	theme	aluminum	217:224	arg1	consequence					249:259	a consequence	247:259	a consequence of the interaction between Al3+ and cell wall composition	247:317	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	50	theme	aluminum	217:224	arg1	[Al3+					226:230	abundant soluble aluminum [Al3+]	200:231	abundant soluble aluminum [Al3+]	200:231	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	1	50	theme	aluminum	217:224	arg1	pH					188:189	low pH	184:189	low pH [H+]	184:194	Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition.					
32480237	4	51	theme	growth	883:888	arg1	reduction					865:873	effective reduction	855:873	effective reduction of root growth only at pH 3.5	855:903	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	4	52	theme	effective	855:863	arg1	reduction					865:873	effective reduction	855:873	effective reduction of root growth only at pH 3.5	855:903	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	6	53	theme	usual	1053:1057	arg1	linkage					1059:1065	the usual linkage	1049:1065	the usual linkage between Ca2+ and pectins	1049:1090	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
32480237	4	54	with	tolerant	824:831	arg1	reduction					865:873	effective reduction	855:873	effective reduction of root growth only at pH 3.5	855:903	Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5.					
32480237	3	55	dep	composition	514:524	arg1	the					510:512	the	510:512	the	510:512	Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h.					
32480237	6	56	theme	cell	1315:1318	arg1	walls					1320:1324	cell walls	1315:1324	cell walls	1315:1324	Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.					
34800523	7	0	theme	impedimetric	806:817	arg1	change					819:824	impedimetric change	806:824	impedimetric change in response to humidity variation	806:858	The mechanism of sensing is based on impedimetric change in response to humidity variation.					
34800523	10	1	theme	several	1096:1102	arg1	devices					1104:1110	several devices	1096:1110	several devices	1096:1110	The fabricated sensors are very attractive to use in several devices for environmental monitoring and biomedical applications.					
34800523	5	2	theme	13.0 mm	515:521	arg1	diameter					523:530	13.0 mm diameter	515:530	13.0 mm diameter	515:530	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness were prepared using hydraulic press under the pressure of 375 MPa.					
34800523	4	3	theme	mixing	485:490	arg1	technique					493:501	echo-friendly (mechanical mixing) technique	459:501	echo-friendly (mechanical mixing) technique	459:501	Various composition of chitosan-CeO2-CdO nanocomposites were developed using echo-friendly (mechanical mixing) technique.					
34800523	5	4	theme	diameter	523:530	arg1	Pellets					504:510	Pellets	504:510	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness	504:557	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness were prepared using hydraulic press under the pressure of 375 MPa.					
34800523	9	5	theme	Response	952:959	arg1	1 s					990:992	1 s	990:992	1 s	990:992	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	9	5	theme	Response	952:959	arg1	times					974:978	Response and recovery times	952:978	times	974:978	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	5	6	theme	375 MPa	617:623	arg1	pressure					605:612	the pressure	601:612	the pressure of 375 MPa	601:623	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness were prepared using hydraulic press under the pressure of 375 MPa.					
34800523	10	7	theme	biomedical	1145:1154	arg1	applications					1156:1167	biomedical applications	1145:1167	biomedical applications	1145:1167	The fabricated sensors are very attractive to use in several devices for environmental monitoring and biomedical applications.					
34800523	5	8	theme	hydraulic	579:587	arg1	press					589:593	hydraulic press	579:593	hydraulic press	579:593	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness were prepared using hydraulic press under the pressure of 375 MPa.					
34800523	9	9	theme	humidity	1005:1012	arg1	range					1022:1026	the humidity sensing range	1001:1026	the humidity sensing range	1001:1026	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	9	9	theme	humidity	1005:1012	arg1	RH					1039:1040	RH	1039:1040	RH	1039:1040	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	6	10	used	used	643:646	arg2	paste					633:637	Silver paste	626:637	Silver paste	626:637	Silver paste was used to deposit the electrodes; the length of each electrode was 12.0 mm and the gap between two electrodes was 2.0 ± 0.5 mm.					
34800523	8	11	theme	%	947:947	arg1	RH					948:949	-2091.1 kΩ/%RH	936:949	-2091.1 kΩ/%RH	936:949	Fabricated sensors showed high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH.					
34800523	9	12	theme	sensing	1014:1020	arg1	range					1022:1026	the humidity sensing range	1001:1026	the humidity sensing range	1001:1026	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	9	12	theme	sensing	1014:1020	arg1	RH					1039:1040	RH	1039:1040	RH	1039:1040	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	8	13	theme	high	887:890	arg1	sensitivities					892:904	high sensitivities	887:904	high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH	887:949	Fabricated sensors showed high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH.					
34800523	4	14	theme	echo-friendly	459:471	arg1	technique					493:501	echo-friendly (mechanical mixing) technique	459:501	echo-friendly (mechanical mixing) technique	459:501	Various composition of chitosan-CeO2-CdO nanocomposites were developed using echo-friendly (mechanical mixing) technique.					
34800523	10	15	from	use	1089:1091	arg1	devices					1104:1110	several devices	1096:1110	several devices	1096:1110	The fabricated sensors are very attractive to use in several devices for environmental monitoring and biomedical applications.					
34800523	6	16	theme	electrode	694:702	arg1	12.0 mm					708:714	12.0 mm	708:714	12.0 mm	708:714	Silver paste was used to deposit the electrodes; the length of each electrode was 12.0 mm and the gap between two electrodes was 2.0 ± 0.5 mm.					
34800523	6	16	theme	electrode	694:702	arg1	length					679:684	the length	675:684	the length of each electrode	675:702	Silver paste was used to deposit the electrodes; the length of each electrode was 12.0 mm and the gap between two electrodes was 2.0 ± 0.5 mm.					
34800523	10	17	theme	environmental	1116:1128	arg1	monitoring					1130:1139	environmental monitoring	1116:1139	environmental monitoring	1116:1139	The fabricated sensors are very attractive to use in several devices for environmental monitoring and biomedical applications.					
34800523	9	18	dep	95	1036:1037	arg1	to					1033:1034	to	1033:1034	to	1033:1034	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	1	19	theme	impedimetric	213:224	arg1	sensors					235:241	impedimetric humidity sensors	213:241	impedimetric humidity sensors	213:241	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	4	20	theme	mechanical	474:483	arg1	technique					493:501	echo-friendly (mechanical mixing) technique	459:501	echo-friendly (mechanical mixing) technique	459:501	Various composition of chitosan-CeO2-CdO nanocomposites were developed using echo-friendly (mechanical mixing) technique.					
34800523	1	21	theme	chitosan	123:130	arg1	chitosan					123:130	chitosan	123:130	chitosan	123:130	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	1	21	theme	chitosan	123:130	arg1	Nanocomposites					105:118	Nanocomposites	105:118	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	105:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	1	21	theme	chitosan	123:130	arg1	nanopowder					174:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	1	22	theme	humidity	226:233	arg1	sensors					235:241	impedimetric humidity sensors	213:241	impedimetric humidity sensors	213:241	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	9	23	dep	1 s	990:992	arg1	up					984:985	up	984:985	up	984:985	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	7	24	theme	sensing	786:792	arg1	mechanism					773:781	The mechanism	769:781	The mechanism of sensing	769:792	The mechanism of sensing is based on impedimetric change in response to humidity variation.					
34800523	1	25	theme	cerium	136:141	arg1	nanopowder					174:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	2	26	used	used	277:280	arg2	temperature-stirring					252:271	The low temperature-stirring	244:271	The low temperature-stirring	244:271	The low temperature-stirring was used to synthesize CeO2-CdO nanopowder.					
34800523	3	27	theme	Average	317:323	arg1	size					334:337	Average particle size	317:337	Average particle size of synthesized nanopowder	317:363	Average particle size of synthesized nanopowder was 100 ± 20 nm.					
34800523	8	28	theme	kΩ/	944:946	arg1	%					947:947	%	947:947	%	947:947	Fabricated sensors showed high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH.					
34800523	0	29	theme	chitosan-CeO2-CdO	36:52	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan-CeO2-CdO nanocomposite based impedimetric humidity sensors.					
34800523	0	29	theme	chitosan-CeO2-CdO	36:52	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan-CeO2-CdO nanocomposite based impedimetric humidity sensors.					
34800523	3	30	theme	particle	325:332	arg1	size					334:337	Average particle size	317:337	Average particle size of synthesized nanopowder	317:363	Average particle size of synthesized nanopowder was 100 ± 20 nm.					
34800523	10	31	theme	fabricated	1047:1056	arg1	sensors					1058:1064	The fabricated sensors	1043:1064	The fabricated sensors	1043:1064	The fabricated sensors are very attractive to use in several devices for environmental monitoring and biomedical applications.					
34800523	10	31	theme	fabricated	1047:1056	arg1	attractive					1075:1084	attractive	1075:1084	attractive	1075:1084	The fabricated sensors are very attractive to use in several devices for environmental monitoring and biomedical applications.					
34800523	4	32	theme	nanocomposites	423:436	arg1	composition					390:400	Various composition	382:400	Various composition of chitosan-CeO2-CdO nanocomposites	382:436	Various composition of chitosan-CeO2-CdO nanocomposites were developed using echo-friendly (mechanical mixing) technique.					
34800523	0	33	theme	impedimetric	74:85	arg1	sensors					96:102	based impedimetric humidity sensors	68:102	based impedimetric humidity sensors	68:102	Fabrication and characterization of chitosan-CeO2-CdO nanocomposite based impedimetric humidity sensors.					
34800523	4	34	theme	chitosan-CeO2-CdO	405:421	arg1	nanocomposites					423:436	chitosan-CeO2-CdO nanocomposites	405:436	chitosan-CeO2-CdO nanocomposites	405:436	Various composition of chitosan-CeO2-CdO nanocomposites were developed using echo-friendly (mechanical mixing) technique.					
34800523	1	35	theme	oxide‑cadmium	143:155	arg1	nanopowder					174:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	0	36	theme	based	68:72	arg1	sensors					96:102	based impedimetric humidity sensors	68:102	based impedimetric humidity sensors	68:102	Fabrication and characterization of chitosan-CeO2-CdO nanocomposite based impedimetric humidity sensors.					
34800523	9	37	theme	recovery	965:972	arg1	1 s					990:992	1 s	990:992	1 s	990:992	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	9	37	theme	recovery	965:972	arg1	times					974:978	Response and recovery times	952:978	times	974:978	Response and recovery times are up to 1 s, while the humidity sensing range is 5 to 95%RH.					
34800523	2	38	theme	low	248:250	arg1	temperature-stirring					252:271	The low temperature-stirring	244:271	The low temperature-stirring	244:271	The low temperature-stirring was used to synthesize CeO2-CdO nanopowder.					
34800523	1	39	theme	oxide	157:161	arg1	nanopowder					174:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	3	40	theme	synthesized	342:352	arg1	nanopowder					354:363	synthesized nanopowder	342:363	synthesized nanopowder	342:363	Average particle size of synthesized nanopowder was 100 ± 20 nm.					
34800523	5	41	theme	1.0 ± 0.1 mm	536:547	arg1	thickness					549:557	1.0 ± 0.1 mm thickness	536:557	1.0 ± 0.1 mm thickness	536:557	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness were prepared using hydraulic press under the pressure of 375 MPa.					
34800523	3	42	theme	nanopowder	354:363	arg1	size					334:337	Average particle size	317:337	Average particle size of synthesized nanopowder	317:363	Average particle size of synthesized nanopowder was 100 ± 20 nm.					
34800523	4	43	theme	Various	382:388	arg1	composition					390:400	Various composition	382:400	Various composition of chitosan-CeO2-CdO nanocomposites	382:436	Various composition of chitosan-CeO2-CdO nanocomposites were developed using echo-friendly (mechanical mixing) technique.					
34800523	1	44	theme	CeO2-CdO	164:171	arg1	nanopowder					174:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	5	45	theme	thickness	549:557	arg1	Pellets					504:510	Pellets	504:510	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness	504:557	Pellets of 13.0 mm diameter and 1.0 ± 0.1 mm thickness were prepared using hydraulic press under the pressure of 375 MPa.					
34800523	7	46	theme	humidity	841:848	arg1	variation					850:858	humidity variation	841:858	humidity variation	841:858	The mechanism of sensing is based on impedimetric change in response to humidity variation.					
34800523	7	47	from	change	819:824	arg1	response					829:836	response	829:836	response to humidity variation	829:858	The mechanism of sensing is based on impedimetric change in response to humidity variation.					
34800523	8	48	theme	-2091.1	936:942	arg1	%					947:947	%	947:947	%	947:947	Fabricated sensors showed high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH.					
34800523	2	49	theme	CeO2-CdO	296:303	arg1	nanopowder					305:314	CeO2-CdO nanopowder	296:314	CeO2-CdO nanopowder	296:314	The low temperature-stirring was used to synthesize CeO2-CdO nanopowder.					
34800523	8	50	theme	kΩ/	926:928	arg1	%					929:929	-930.0 kΩ/%RH	919:931	-930.0 kΩ/%RH	919:931	Fabricated sensors showed high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH.					
34800523	0	51	theme	humidity	87:94	arg1	sensors					96:102	based impedimetric humidity sensors	68:102	based impedimetric humidity sensors	68:102	Fabrication and characterization of chitosan-CeO2-CdO nanocomposite based impedimetric humidity sensors.					
34800523	8	52	theme	Fabricated	861:870	arg1	sensors					872:878	Fabricated sensors	861:878	Fabricated sensors	861:878	Fabricated sensors showed high sensitivities ranging from -930.0 kΩ/%RH to -2091.1 kΩ/%RH.					
34800523	1	53	theme	nanopowder	174:183	arg1	chitosan					123:130	chitosan	123:130	chitosan	123:130	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	1	53	theme	nanopowder	174:183	arg1	Nanocomposites					105:118	Nanocomposites	105:118	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	105:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	1	53	theme	nanopowder	174:183	arg1	nanopowder					174:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder	136:183	Nanocomposites of chitosan and cerium oxide‑cadmium oxide (CeO2-CdO) nanopowder were developed to fabricate impedimetric humidity sensors.					
34800523	6	54	theme	Silver	626:631	arg1	paste					633:637	Silver paste	626:637	Silver paste	626:637	Silver paste was used to deposit the electrodes; the length of each electrode was 12.0 mm and the gap between two electrodes was 2.0 ± 0.5 mm.					
33798571	3	0	theme	high	328:331	arg1	hydrogels					340:348	superabsorbent high porous hydrogels	313:348	superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid	313:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	5	1	from	hydrogels	929:937	arg1	relation					942:949	relation	942:949	relation with their composition, which endows them tunable properties and versatility	942:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	4	2	from	tests	746:750	arg1	environment					769:779	physiological environment	755:779	physiological environment	755:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	3	3	theme	porous	333:338	arg1	hydrogels					340:348	superabsorbent high porous hydrogels	313:348	superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid	313:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	1	4	theme	bio-applications	163:178	arg1	use					180:182	bio-applications use	163:182	bio-applications use	163:182	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	4	5	theme	resulting	532:540	arg1	hydrogels					542:550	The resulting hydrogels	528:550	The resulting hydrogels obtained with different ratios between alginate and phytic acid	528:614	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	4	6	theme	simultaneous	814:825	arg1	system					839:844	a simultaneous TG/FT-IR/MS system	812:844	a simultaneous TG/FT-IR/MS system	812:844	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	3	7	theme	physical	375:382	arg1	alginate					366:373	sodium alginate physical	359:382	sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid	359:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	1	8	theme	Alginate	62:69	arg1	hydrogels					71:79	Alginate hydrogels	62:79	Alginate hydrogels	62:79	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	1	8	theme	Alginate	62:69	arg1	biomaterials					118:129	extremely versatile and flexible biomaterials	85:129	extremely versatile and flexible biomaterials	85:129	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	4	9	from	measurements	723:734	arg1	environment					769:779	physiological environment	755:779	physiological environment	755:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	4	10	from	microscopy	707:716	arg1	environment					769:779	physiological environment	755:779	physiological environment	755:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	5	11	from	differences	879:889	arg1	properties					911:920	physico-chemical properties	894:920	physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility	894:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	5	12	dep	are	853:855	arg1	put					857:859	put	857:859	are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility	853:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	5	13	theme	physico-chemical	894:909	arg1	properties					911:920	physico-chemical properties	894:920	physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility	894:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	2	14	theme	tissue	278:283	arg1	regeneration					285:296	tissue regeneration	278:296	tissue regeneration	278:296	Their similarity with extracellular matrix is a key factor in their performance for cell and tissue regeneration.					
33798571	3	15	theme	sodium	359:364	arg1	alginate					366:373	sodium alginate physical	359:382	sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid	359:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	3	16	dep	compound	423:430	arg1	acid					446:449	phytic acid	439:449	a natural crosslinker compound namely phytic acid	401:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	4	17	theme	TG/FT-IR/MS	827:837	arg1	system					839:844	a simultaneous TG/FT-IR/MS system	812:844	a simultaneous TG/FT-IR/MS system	812:844	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	5	18	theme	hydrogels	929:937	arg1	properties					911:920	physico-chemical properties	894:920	physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility	894:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	1	19	with	biomaterials	118:129	arg1	potential					149:157	an enormous potential	137:157	an enormous potential for bio-applications use	137:182	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	2	20	from	factor	237:242	arg1	performance					253:263	their performance	247:263	their performance	247:263	Their similarity with extracellular matrix is a key factor in their performance for cell and tissue regeneration.					
33798571	3	21	theme	natural	403:409	arg1	compound					423:430	a natural crosslinker compound	401:430	a natural crosslinker compound namely phytic acid	401:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	4	22	from	analysis	794:801	arg1	environment					769:779	physiological environment	755:779	physiological environment	755:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	1	23	theme	versatile	95:103	arg1	hydrogels					71:79	Alginate hydrogels	62:79	Alginate hydrogels	62:79	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	1	23	theme	versatile	95:103	arg1	biomaterials					118:129	extremely versatile and flexible biomaterials	85:129	extremely versatile and flexible biomaterials	85:129	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	4	24	theme	XRD	719:721	arg1	measurements					723:734	XRD measurements	719:734	XRD measurements	719:734	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	0	25	theme	phytic	23:28	arg1	acid					30:33	phytic acid	23:33	phytic acid for hydrogels preparation	23:59	Alginate enriched with phytic acid for hydrogels preparation.					
33798571	4	26	theme	thermal	786:792	arg1	analysis					794:801	thermal analysis	786:801	thermal analysis	786:801	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	4	27	theme	phytic	604:609	arg1	acid					611:614	phytic acid	604:614	phytic acid	604:614	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	1	28	theme	flexible	109:116	arg1	hydrogels					71:79	Alginate hydrogels	62:79	Alginate hydrogels	62:79	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	1	28	theme	flexible	109:116	arg1	biomaterials					118:129	extremely versatile and flexible biomaterials	85:129	extremely versatile and flexible biomaterials	85:129	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	4	29	theme	swelling	737:744	arg1	tests					746:750	swelling tests	737:750	swelling tests in physiological environment	737:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	4	30	theme	physiological	755:767	arg1	environment					769:779	physiological environment	755:779	physiological environment	755:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	4	31	theme	electron	698:705	arg1	microscopy					707:716	scanning electron microscopy	689:716	scanning electron microscopy	689:716	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	3	32	theme	phytic	439:444	arg1	acid					446:449	phytic acid	439:449	a natural crosslinker compound namely phytic acid	401:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	5	33	from	properties	911:920	arg1	relation					942:949	relation	942:949	relation with their composition, which endows them tunable properties and versatility	942:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	4	34	theme	scanning	689:696	arg1	microscopy					707:716	scanning electron microscopy	689:716	scanning electron microscopy	689:716	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	3	35	theme	crosslinker	411:421	arg1	compound					423:430	a natural crosslinker compound	401:430	a natural crosslinker compound namely phytic acid	401:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	2	36	theme	key	233:235	arg1	factor					237:242	a key factor	231:242	a key factor in their performance for cell and tissue regeneration	231:296	Their similarity with extracellular matrix is a key factor in their performance for cell and tissue regeneration.					
33798571	2	36	theme	key	233:235	arg1	similarity					191:200	Their similarity	185:200	Their similarity with extracellular matrix	185:226	Their similarity with extracellular matrix is a key factor in their performance for cell and tissue regeneration.					
33798571	3	37	theme	specific	507:514	arg1	properties					516:525	their specific properties	501:525	their specific properties	501:525	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	0	38	theme	hydrogels	39:47	arg1	preparation					49:59	hydrogels preparation	39:59	hydrogels preparation	39:59	Alginate enriched with phytic acid for hydrogels preparation.					
33798571	3	39	theme	superabsorbent	313:326	arg1	hydrogels					340:348	superabsorbent high porous hydrogels	313:348	superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid	313:449	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	4	40	dep	spectroscopy	665:676	arg1	technique					678:686	technique	678:686	technique	678:686	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	3	41	theme	properties	516:525	arg1	viewpoint					488:496	the viewpoint	484:496	the viewpoint of their specific properties	484:525	In this study superabsorbent high porous hydrogels based on sodium alginate physical crosslinked with a natural crosslinker compound namely phytic acid were prepared and evaluated from the viewpoint of their specific properties.					
33798571	4	42	theme	different	566:574	arg1	ratios					576:581	different ratios	566:581	different ratios between alginate and phytic acid	566:614	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	2	43	with	similarity	191:200	arg1	matrix					221:226	extracellular matrix	207:226	extracellular matrix	207:226	Their similarity with extracellular matrix is a key factor in their performance for cell and tissue regeneration.					
33798571	2	44	theme	extracellular	207:219	arg1	matrix					221:226	extracellular matrix	207:226	extracellular matrix	207:226	Their similarity with extracellular matrix is a key factor in their performance for cell and tissue regeneration.					
33798571	5	45	dep	them	988:991	arg1	versatility					1016:1026	versatility	1016:1026	versatility	1016:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	5	45	dep	them	988:991	arg1	properties					1001:1010	tunable properties	993:1010	tunable properties	993:1010	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	5	46	theme	tunable	993:999	arg1	properties					1001:1010	tunable properties	993:1010	tunable properties	993:1010	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	4	47	from	spectroscopy	665:676	arg1	environment					769:779	physiological environment	755:779	physiological environment	755:779	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	4	48	dep	Fourier	638:644	arg1	transform					646:654	transform	646:654	transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis	646:801	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
33798571	5	49	from	relation	942:949	arg1	properties					911:920	physico-chemical properties	894:920	physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility	894:1026	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	1	50	theme	enormous	140:147	arg1	potential					149:157	an enormous potential	137:157	an enormous potential for bio-applications use	137:182	Alginate hydrogels are extremely versatile and flexible biomaterials, with an enormous potential for bio-applications use.					
33798571	5	51	with	relation	942:949	arg1	composition					962:972	their composition	956:972	their composition	956:972	There are put into evidence the differences in physico-chemical properties of the hydrogels in relation with their composition, which endows them tunable properties and versatility.					
33798571	4	52	theme	infrared	656:663	arg1	spectroscopy					665:676	infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis	656:801	spectroscopy	665:676	The resulting hydrogels obtained with different ratios between alginate and phytic acid were characterized by Fourier transform infrared spectroscopy technique, scanning electron microscopy, XRD measurements, swelling tests in physiological environment, and thermal analysis by using a simultaneous TG/FT-IR/MS system.					
34822996	5	0	contain	had	1155:1157	arg1	Shrimp					1126:1131	Shrimp	1126:1131	Shrimp in the SYN group	1126:1148	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	5	0	contain	had	1155:1157	arg2	gain					1189:1192	a significantly higher weight gain	1159:1192	a significantly higher weight gain	1159:1192	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	5	0	contain	had	1155:1157	arg2	weight					1210:1215	total final weight	1198:1215	total final weight	1198:1215	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	8	1	from	lgbp	1884:1887	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	1	from	lgbp	1884:1887	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	1	from	lgbp	1884:1887	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	1	from	lgbp	1884:1887	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	1	from	lgbp	1884:1887	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	1	from	lgbp	1884:1887	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	5	2	theme	higher	1175:1180	arg1	gain					1189:1192	a significantly higher weight gain	1159:1192	a significantly higher weight gain	1159:1192	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	0	3	theme	growth	178:183	arg1	performance					185:195	the growth performance and health status	174:213	performance	185:195	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	4	theme	probiotic	404:412	arg1	7-40					438:441	probiotic Lactobacillus plantarum 7-40	404:441	probiotic Lactobacillus plantarum 7-40	404:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	5	5	theme	final	1204:1208	arg1	weight					1210:1215	total final weight	1198:1215	total final weight	1198:1215	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	2	6	theme	probiotic	571:579	arg1	growth					561:566	the growth	557:566	the growth of probiotic	557:579	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	8	7	from	propoii	1894:1900	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	7	from	propoii	1894:1900	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	7	from	propoii	1894:1900	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	7	from	propoii	1894:1900	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	7	from	propoii	1894:1900	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	7	from	propoii	1894:1900	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	8	from	burst	1790:1794	arg1	haemocytes					1868:1877	the haemocytes	1864:1877	the haemocytes	1864:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	9	contain	had	1655:1657	arg1	Shrimps					1630:1636	Shrimps	1630:1636	Shrimps in the SYN group	1630:1653	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	9	contain	had	1655:1657	arg2	mortality					1690:1698	significantly lower cumulative mortality	1659:1698	significantly lower cumulative mortality	1659:1698	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	4	10	theme	higher	1051:1056	arg1	survival					1058:1065	significantly higher survival	1037:1065	significantly higher survival	1037:1065	Shrimp fed the ME, LP, and SYN diets had significantly higher survival than that of shrimp fed with the control diet for 8 weeks.					
34822996	8	11	from	sp	1890:1891	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	11	from	sp	1890:1891	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	11	from	sp	1890:1891	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	11	from	sp	1890:1891	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	11	from	sp	1890:1891	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	11	from	sp	1890:1891	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	0	12	theme	probiotic	107:115	arg1	mushroom					75:82	king oyster mushroom	63:82	king oyster mushroom	63:82	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	13	theme	oyster	357:362	arg1	mushroom					364:371	king oyster mushroom	352:371	king oyster mushroom	352:371	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	6	14	theme	acid	1302:1305	arg1	bacteria					1307:1314	lactic acid bacteria	1295:1314	lactic acid bacteria count	1295:1320	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	5	15	from	Shrimp	1126:1131	arg1	group					1144:1148	the SYN group	1136:1148	the SYN group	1136:1148	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	3	16	theme	basal	788:792	arg1	diet					794:797	a basal diet	786:797	a basal diet supplemented with KOME (5 g kg-1) (ME)	786:836	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	6	17	theme	bacteria	1389:1396	arg1	count					1398:1402	the Vibrio-like bacteria count	1373:1402	the Vibrio-like bacteria count	1373:1402	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	6	17	theme	bacteria	1389:1396	arg1	higher					1422:1427	higher	1422:1427	higher	1422:1427	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	0	18	dep	improve	166:172	arg1	diet					158:161	diet	158:161	diet	158:161	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	19	theme	SYN	1645:1647	arg1	group					1649:1653	the SYN group	1641:1653	the SYN group	1641:1653	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	5	20	dep	control	1240:1246	arg1	the					1236:1238	the	1236:1238	the	1236:1238	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	2	21	theme	V.	632:633	arg1	alginolyticus					635:647	V. alginolyticus	632:647	V. alginolyticus	632:647	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	8	22	from	phenoloxidase	1763:1775	arg1	haemocytes					1868:1877	the haemocytes	1864:1877	the haemocytes	1864:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	23	from	pen3a	1909:1913	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	23	from	pen3a	1909:1913	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	23	from	pen3a	1909:1913	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	23	from	pen3a	1909:1913	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	23	from	pen3a	1909:1913	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	23	from	pen3a	1909:1913	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	24	theme	immune	1735:1740	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	24	theme	immune	1735:1740	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	24	theme	immune	1735:1740	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	24	theme	immune	1735:1740	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	24	theme	immune	1735:1740	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	1	25	theme	composite	310:318	arg1	extract					323:329	a synbiotic composite an extract	298:329	a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	298:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	26	theme	lysozyme	1801:1808	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	6	27	theme	SYN	1354:1356	arg1	group					1358:1362	the SYN group	1350:1362	the SYN group	1350:1362	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	3	28	theme	KOME	817:820	arg1	 					832:832	KOME (5 g kg-1) 	817:832	KOME (5 g kg-1) (ME)	817:836	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	3	28	theme	KOME	817:820	arg1	ME					834:835	ME	834:835	ME	834:835	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	4	29	theme	ME	1011:1012	arg1	LP					1015:1016	the ME, LP, and SYN diets	1007:1031	LP	1015:1016	Shrimp fed the ME, LP, and SYN diets had significantly higher survival than that of shrimp fed with the control diet for 8 weeks.					
34822996	0	30	theme	oyster	68:73	arg1	mushroom					75:82	king oyster mushroom	63:82	king oyster mushroom	63:82	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	31	theme	extract	323:329	arg1	effects					287:293	the effects	283:293	the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp	283:501	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	0	32	theme	shrimp	224:229	arg1	performance					185:195	the growth performance and health status	174:213	performance	185:195	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	0	32	theme	shrimp	224:229	arg1	status					208:213	the growth performance and health status	174:213	status	208:213	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	7	33	theme	health	1480:1485	arg1	assessment					1494:1503	the health status assessment	1476:1503	the health status assessment	1476:1503	For the health status assessment, the disease resistance of shrimp against V. alginolyticus was improved in all treatments compared to the shrimp in control.					
34822996	5	34	theme	other	1252:1256	arg1	treatments					1258:1267	other treatments	1252:1267	other treatments	1252:1267	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	5	35	theme	SYN	1140:1142	arg1	group					1144:1148	the SYN group	1136:1148	the SYN group	1136:1148	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	8	36	theme	respiratory	1778:1788	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	3	37	theme	5 g kg-1	823:830	arg1	 					832:832	KOME (5 g kg-1) 	817:832	KOME (5 g kg-1) (ME)	817:836	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	3	37	theme	5 g kg-1	823:830	arg1	ME					834:835	ME	834:835	ME	834:835	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	9	38	theme	same	2192:2195	arg1	time					2197:2200	the same time	2188:2200	the same time	2188:2200	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	9	39	used	used	2067:2070	arg2	synbiotic					2077:2085	a synbiotic	2075:2085	a synbiotic	2075:2085	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	9	39	used	used	2067:2070	arg2	combination					2025:2035	a combination	2023:2035	a combination of KOME and probiotics	2023:2058	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	8	40	theme	pexn	1847:1850	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	40	theme	pexn	1847:1850	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	40	theme	pexn	1847:1850	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	40	theme	pexn	1847:1850	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	2	41	theme	Vibrio	604:609	arg1	pathogens					611:619	Vibrio pathogens	604:619	Vibrio pathogens	604:619	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	2	41	theme	Vibrio	604:609	arg1	harveyi					678:684	V. harveyi	675:684	V. harveyi	675:684	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	2	41	theme	Vibrio	604:609	arg1	alginolyticus					635:647	V. alginolyticus	632:647	V. alginolyticus	632:647	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	2	41	theme	Vibrio	604:609	arg1	parahaemolyticus					653:668	V. parahaemolyticus	650:668	V. parahaemolyticus	650:668	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	0	42	theme	containing	12:21	arg1	prebiotic					23:31	A synbiotic containing prebiotic	0:31	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum	0:140	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	43	theme	pen4	1856:1859	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	43	theme	pen4	1856:1859	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	43	theme	pen4	1856:1859	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	43	theme	pen4	1856:1859	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	44	from	Shrimps	1630:1636	arg1	group					1649:1653	the SYN group	1641:1653	the SYN group	1641:1653	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	4	45	theme	control	1100:1106	arg1	diet					1108:1111	the control diet	1096:1111	the control diet	1096:1111	Shrimp fed the ME, LP, and SYN diets had significantly higher survival than that of shrimp fed with the control diet for 8 weeks.					
34822996	8	46	from	pexn	1903:1906	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	46	from	pexn	1903:1906	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	46	from	pexn	1903:1906	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	46	from	pexn	1903:1906	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	46	from	pexn	1903:1906	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	46	from	pexn	1903:1906	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	1	47	from	by-product	338:347	arg1	extract					323:329	a synbiotic composite an extract	298:329	a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	298:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	47	from	by-product	338:347	arg1	effects					287:293	the effects	283:293	the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp	283:501	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	9	48	theme	circular	2234:2241	arg1	economy					2243:2249	the circular economy	2230:2249	the circular economy	2230:2249	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	3	49	theme	basal	841:845	arg1	diet					847:850	a basal diet	839:850	a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP)	839:902	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	9	50	theme	infectious	2144:2153	arg1	diseases					2155:2162	infectious diseases	2144:2162	infectious diseases caused by Vibrio	2144:2179	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	5	51	theme	total	1198:1202	arg1	weight					1210:1215	total final weight	1198:1215	total final weight	1198:1215	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	1	52	theme	growth	450:455	arg1	performance					457:467	the growth performance and health status	446:485	performance	457:467	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	53	dep	Pleurotus	374:382	arg1	eryngii					384:390	Pleurotus eryngii (KOME)	374:397	Pleurotus eryngii (KOME)	374:397	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	54	from	gpx	1926:1928	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	54	from	gpx	1926:1928	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	54	from	gpx	1926:1928	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	54	from	gpx	1926:1928	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	54	from	gpx	1926:1928	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	54	from	gpx	1926:1928	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	9	55	theme	KOME	2040:2043	arg1	combination					2025:2035	a combination	2023:2035	a combination of KOME and probiotics	2023:2058	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	9	55	theme	KOME	2040:2043	arg1	synbiotic					2077:2085	a synbiotic	2075:2085	a synbiotic	2075:2085	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	1	56	theme	shrimp	496:501	arg1	performance					457:467	the growth performance and health status	446:485	performance	457:467	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	56	theme	shrimp	496:501	arg1	status					480:485	the growth performance and health status	446:485	status	480:485	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	9	57	theme	probiotics	2049:2058	arg1	combination					2025:2035	a combination	2023:2035	a combination of KOME and probiotics	2023:2058	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	9	57	theme	probiotics	2049:2058	arg1	synbiotic					2077:2085	a synbiotic	2075:2085	a synbiotic	2075:2085	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	8	58	from	increase	1723:1730	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	58	from	increase	1723:1730	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	58	from	increase	1723:1730	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	58	from	increase	1723:1730	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	58	from	increase	1723:1730	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	58	from	increase	1723:1730	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	1	59	from	effects	287:293	arg1	by-product					338:347	a by-product	336:347	a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	336:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	59	from	effects	287:293	arg1	performance					457:467	the growth performance and health status	446:485	performance	457:467	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	59	from	effects	287:293	arg1	status					480:485	the growth performance and health status	446:485	status	480:485	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	0	60	theme	health	201:206	arg1	status					208:213	the growth performance and health status	174:213	status	208:213	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	61	theme	Lactobacillus	414:426	arg1	7-40					438:441	probiotic Lactobacillus plantarum 7-40	404:441	probiotic Lactobacillus plantarum 7-40	404:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	3	62	theme	basal	911:915	arg1	diet					917:920	a basal diet	909:920	a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN)	909:993	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	8	63	theme	lower	1673:1677	arg1	mortality					1690:1698	significantly lower cumulative mortality	1659:1698	significantly lower cumulative mortality	1659:1698	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	1	64	theme	7-40	438:441	arg1	by-product					338:347	a by-product	336:347	a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	336:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	7	65	theme	shrimp	1532:1537	arg1	resistance					1518:1527	the disease resistance	1506:1527	the disease resistance of shrimp against V. alginolyticus	1506:1562	For the health status assessment, the disease resistance of shrimp against V. alginolyticus was improved in all treatments compared to the shrimp in control.					
34822996	6	66	theme	bacteria	1307:1314	arg1	count					1316:1320	lactic acid bacteria count	1295:1320	lactic acid bacteria count	1295:1320	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	4	67	contain	had	1033:1035	arg2	survival					1058:1065	significantly higher survival	1037:1065	significantly higher survival	1037:1065	Shrimp fed the ME, LP, and SYN diets had significantly higher survival than that of shrimp fed with the control diet for 8 weeks.					
34822996	4	67	contain	had	1033:1035	arg1	Shrimp					996:1001	Shrimp	996:1001	Shrimp fed the ME, LP, and SYN diets	996:1031	Shrimp fed the ME, LP, and SYN diets had significantly higher survival than that of shrimp fed with the control diet for 8 weeks.					
34822996	1	68	theme	mushroom	364:371	arg1	by-product					338:347	a by-product	336:347	a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	336:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	9	69	theme	growth	2102:2107	arg1	performance					2109:2119	the growth performance	2098:2119	the growth performance	2098:2119	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	6	70	theme	lactic	1295:1300	arg1	bacteria					1307:1314	lactic acid bacteria	1295:1314	lactic acid bacteria count	1295:1320	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	2	71	theme	V.	650:651	arg1	parahaemolyticus					653:668	V. parahaemolyticus	650:668	V. parahaemolyticus	650:668	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	8	72	theme	significant	1711:1721	arg1	increase					1723:1730	the significant increase	1707:1730	the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes,	1707:1878	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	1	73	theme	Pleurotus	374:382	arg1	by-product					338:347	a by-product	336:347	a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	336:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	6	74	theme	Vibrio-like	1377:1387	arg1	count					1398:1402	the Vibrio-like bacteria count	1373:1402	the Vibrio-like bacteria count	1373:1402	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	6	74	theme	Vibrio-like	1377:1387	arg1	higher					1422:1427	higher	1422:1427	higher	1422:1427	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	6	75	theme	control	1457:1463	arg1	group					1465:1469	the control group	1453:1469	the control group	1453:1469	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	4	76	theme	SYN	1023:1025	arg1	diets					1027:1031	the ME, LP, and SYN diets	1007:1031	diets	1027:1031	Shrimp fed the ME, LP, and SYN diets had significantly higher survival than that of shrimp fed with the control diet for 8 weeks.					
34822996	0	77	theme	white	218:222	arg1	shrimp					224:229	white shrimp	218:229	white shrimp	218:229	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	78	dep	Lactobacillus	414:426	arg1	plantarum					428:436	plantarum	428:436	plantarum	428:436	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	79	theme	health	473:478	arg1	status					480:485	the growth performance and health status	446:485	status	480:485	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	80	theme	shrimp	1956:1961	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	2	81	theme	V.	675:676	arg1	harveyi					678:684	V. harveyi	675:684	V. harveyi	675:684	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	0	82	theme	king	63:66	arg1	mushroom					75:82	king oyster mushroom	63:82	king oyster mushroom	63:82	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	83	from	pen4	1916:1919	arg1	haepatopancreas					1937:1951	the haepatopancreas	1933:1951	the haepatopancreas of shrimp	1933:1961	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	83	from	pen4	1916:1919	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	83	from	pen4	1916:1919	arg1	activity					1810:1817	lysozyme activity	1801:1817	lysozyme activity	1801:1817	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	83	from	pen4	1916:1919	arg1	phenoloxidase					1763:1775	phenoloxidase	1763:1775	phenoloxidase	1763:1775	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	83	from	pen4	1916:1919	arg1	burst					1790:1794	respiratory burst	1778:1794	respiratory burst	1778:1794	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	8	83	from	pen4	1916:1919	arg1	responses					1742:1750	immune responses	1735:1750	immune responses	1735:1750	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	7	84	theme	disease	1510:1516	arg1	resistance					1518:1527	the disease resistance	1506:1527	the disease resistance of shrimp against V. alginolyticus	1506:1562	For the health status assessment, the disease resistance of shrimp against V. alginolyticus was improved in all treatments compared to the shrimp in control.					
34822996	8	85	from	activity	1810:1817	arg1	haemocytes					1868:1877	the haemocytes	1864:1877	the haemocytes	1864:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	3	86	theme	control	727:733	arg1	diet					735:738	a control diet	725:738	a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN)	725:993	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	0	87	theme	mushroom	75:82	arg1	by-product					49:58	a by-product	47:58	a by-product of king oyster mushroom, Pleurotus eryngii and probiotic,	47:116	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	9	88	theme	diseases	2155:2162	arg1	risk					2136:2139	the risk	2132:2139	the risk of infectious diseases caused by Vibrio	2132:2179	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	8	89	theme	cumulative	1679:1688	arg1	mortality					1690:1698	significantly lower cumulative mortality	1659:1698	significantly lower cumulative mortality	1659:1698	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	3	90	theme	probiotic	775:783	arg1	diet					794:797	a basal diet	786:797	a basal diet supplemented with KOME (5 g kg-1) (ME)	786:836	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	0	91	theme	Pleurotus	85:93	arg1	mushroom					75:82	king oyster mushroom	63:82	king oyster mushroom	63:82	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	6	92	theme	ME	1436:1437	arg1	group					1439:1443	the ME group	1432:1443	the ME group	1432:1443	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	7	93	theme	status	1487:1492	arg1	assessment					1494:1503	the health status assessment	1476:1503	the health status assessment	1476:1503	For the health status assessment, the disease resistance of shrimp against V. alginolyticus was improved in all treatments compared to the shrimp in control.					
34822996	3	94	theme	prebiotic	761:769	arg1	diet					794:797	a basal diet	786:797	a basal diet supplemented with KOME (5 g kg-1) (ME)	786:836	Four diets were formulated, including a control diet supplemented without prebiotic and probiotic, a basal diet supplemented with KOME (5 g kg-1) (ME), a basal diet supplemented with probiotic (1 × 108 CFU kg-1) (LP), and a basal diet supplemented with KOME (5 g kg-1) and probiotic (1 × 108 CFU kg-1) (SYN).					
34822996	0	95	dep	Pleurotus	85:93	arg1	eryngii					95:101	eryngii	95:101	eryngii	95:101	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	0	96	theme	synbiotic	2:10	arg1	prebiotic					23:31	A synbiotic containing prebiotic	0:31	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum	0:140	A synbiotic containing prebiotic prepared from a by-product of king oyster mushroom, Pleurotus eryngii and probiotic, Lactobacillus plantarum incorporated in diet to improve the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	1	97	theme	king	352:355	arg1	mushroom					364:371	king oyster mushroom	352:371	king oyster mushroom	352:371	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	2	98	theme	pathogens	611:619	arg1	growth					594:599	the growth	590:599	the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi	590:684	The KOME was able to stimulate the growth of probiotic, but not the growth of Vibrio pathogens, including V. alginolyticus, V. parahaemolyticus, and V. harveyi.					
34822996	5	99	with	comparison	1220:1229	arg1	control					1240:1246	control	1240:1246	control	1240:1246	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	5	99	with	comparison	1220:1229	arg1	treatments					1258:1267	other treatments	1252:1267	other treatments	1252:1267	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	7	100	from	shrimp	1611:1616	arg1	control					1621:1627	control	1621:1627	control	1621:1627	For the health status assessment, the disease resistance of shrimp against V. alginolyticus was improved in all treatments compared to the shrimp in control.					
34822996	5	101	theme	weight	1182:1187	arg1	gain					1189:1192	a significantly higher weight gain	1159:1192	a significantly higher weight gain	1159:1192	Shrimp in the SYN group also had a significantly higher weight gain and total final weight in comparison with the control and other treatments.					
34822996	8	102	theme	gene	1828:1831	arg1	expression					1833:1842	the gene expression	1824:1842	the gene expression of pexn and pen4 in the haemocytes	1824:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	1	103	theme	synbiotic	300:308	arg1	extract					323:329	a synbiotic composite an extract	298:329	a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40	298:441	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
34822996	8	104	from	expression	1833:1842	arg1	haemocytes					1868:1877	the haemocytes	1864:1877	the haemocytes	1864:1877	Shrimps in the SYN group had significantly lower cumulative mortality due to the significant increase in immune responses, including phenoloxidase, respiratory burst, and lysozyme activity, and the gene expression of pexn and pen4 in the haemocytes, and lgbp, sp, propoii, pexn, pen3a, pen4, and gpx in the haepatopancreas of shrimp as compared to the control.					
34822996	6	105	theme	intestinal	1277:1286	arg1	tract					1288:1292	the intestinal tract	1273:1292	the intestinal tract	1273:1292	In the intestinal tract, lactic acid bacteria count was significantly higher in the SYN group, whereas the Vibrio-like bacteria count was significantly higher in the ME group than in the control group.					
34822996	9	106	dep	improve	2090:2096	arg1	contribute					2216:2225	contribute	2216:2225	significantly contribute to the circular economy	2202:2249	Therefore, it is suggested that a combination of KOME and probiotics can be used as a synbiotic to improve the growth performance and reduce the risk of infectious diseases caused by Vibrio and at the same time significantly contribute to the circular economy.					
34822996	1	107	theme	white	490:494	arg1	shrimp					496:501	white shrimp	490:501	white shrimp	490:501	This study aimed to evaluate the effects of a synbiotic composite an extract from a by-product of king oyster mushroom, Pleurotus eryngii (KOME), and probiotic Lactobacillus plantarum 7-40 on the growth performance and health status of white shrimp, Litopenaeus vannamei.					
32422256	4	0	theme	contact	796:802	arg1	time					804:807	contact time	796:807	contact time	796:807	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	13	1	theme	Na+	2232:2234	arg1	ions					2243:2246	Na+ and K+ ions	2232:2246	Na+ and K+ ions	2232:2246	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	8	2	theme	dye	1552:1554	arg1	process					1567:1573	the MG dye adsorption process	1545:1573	the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1545:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	1	3	theme	AAm	258:260	arg1	montmorillonite					233:247	montmorillonite	233:247	montmorillonite (CMC-g-P(AAm)/MMT)	233:266	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	3	theme	AAm	258:260	arg1	/MMT					262:265	CMC-g-P(AAm)/MMT	250:265	CMC-g-P(AAm)/MMT	250:265	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	2	4	theme	MMT	524:526	arg1	nanoparticles					528:540	MMT nanoparticles	524:540	MMT nanoparticles	524:540	The properties and characterization of the adsorbents were investigated using FTIR, SEM, TGA, and XRD analyzes, and the results showed that MMT nanoparticles were successfully distributed in the hydrogel system.					
32422256	9	5	theme	high	1736:1739	arg1	value					1752:1756	a high adsorption value	1734:1756	a high adsorption value	1734:1756	This indicates that the produced composite has a high adsorption value.					
32422256	3	6	theme	different	656:664	arg1	percentages					673:683	different weight percentages	656:683	different weight percentages	656:683	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	6	7	theme	monolayer	1169:1177	arg1	158.1 mg/g					1311:1320	158.1 mg/g	1311:1320	158.1 mg/g	1311:1320	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	6	7	theme	monolayer	1169:1177	arg1	qmax					1200:1203	qmax	1200:1203	qmax	1200:1203	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	6	7	theme	monolayer	1169:1177	arg1	capacity					1190:1197	The maximum monolayer adsorption capacity	1157:1197	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1157:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	4	8	theme	temperature	783:793	arg1	effect					773:778	the effect	769:778	the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye	769:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	3	9	theme	adsorption	701:710	arg1	efficiency					712:721	the maximum adsorption efficiency	689:721	the maximum adsorption efficiency of MG dye	689:731	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	12	10	from	endothermic	2137:2147	arg1	range					2156:2160	the range	2152:2160	the range of 25-50 °C using both adsorbents	2152:2194	Positive ΔH° indicates that the process is endothermic in the range of 25-50 °C using both adsorbents.					
32422256	13	11	theme	adsorption	2251:2260	arg1	efficiency					2262:2271	adsorption efficiency	2251:2271	adsorption efficiency	2251:2271	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	14	12	theme	Adsorption	2408:2417	arg1	decrease					2430:2437	Adsorption efficiency decrease	2408:2437	Adsorption efficiency decrease	2408:2437	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	4	13	theme	pH	854:855	arg1	effect					773:778	the effect	769:778	the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye	769:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	3	14	theme	dye	729:731	arg1	efficiency					712:721	the maximum adsorption efficiency	689:721	the maximum adsorption efficiency of MG dye	689:731	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	5	15	theme	effective	1115:1123	arg1	role					1125:1128	an effective role	1112:1128	an effective role	1112:1128	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	1	16	theme	dye	349:351	arg1	removal					353:359	malachite green (MG) dye removal	328:359	malachite green (MG) dye removal from aqueous solution	328:381	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	7	17	theme	other	1498:1502	arg1	models					1504:1509	other models	1498:1509	other models	1498:1509	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	4	18	theme	concentration	818:830	arg1	effect					773:778	the effect	769:778	the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye	769:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	10	19	theme	adsorption	1804:1813	arg1	processes					1815:1823	the adsorption processes	1800:1823	the adsorption processes in the range of 25-50 °C	1800:1848	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	1	20	theme	graft	159:163	arg1	CMC-g-P					192:198	CMC-g-P	192:198	CMC-g-P(AAm)	192:203	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	20	theme	graft	159:163	arg1	hydrogel					182:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel	129:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm))	129:204	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	7	21	theme	process	1485:1491	arg1	behavior					1469:1476	the kinetic behavior	1457:1476	the kinetic behavior of the process than other models	1457:1509	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	4	22	theme	MG	835:836	arg1	dye					838:840	MG dye	835:840	MG dye	835:840	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	14	23	theme	dye	2562:2564	arg1	molecule					2566:2573	the MG dye molecule	2555:2573	the MG dye molecule	2555:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	3	24	theme	10 wt	751:755	arg1	MMT					758:760	10 wt% MMT	751:760	10 wt% MMT	751:760	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	13	25	theme	ions	2243:2246	arg1	effect					2222:2227	the concentrations effect	2203:2227	the concentrations effect of Na+ and K+ ions on adsorption efficiency	2203:2271	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	5	26	theme	adsorption	962:971	arg1	process					973:979	the adsorption process	958:979	the adsorption process	958:979	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	4	27	theme	MG	889:890	arg1	dye					892:894	MG dye	889:894	MG dye	889:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	6	28	theme	AAm	1280:1282	arg1	/MMT					1284:1287	CMC-g-P(AAm)/MMT	1272:1287	CMC-g-P(AAm)/MMT	1272:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	5	29	theme	good	1032:1035	arg1	agreement					1037:1045	good agreement	1032:1045	good agreement with the Langmuir isotherm model	1032:1078	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	10	30	theme	25-50 °C	1841:1848	arg1	range					1832:1836	the range	1828:1836	the range of 25-50 °C	1828:1848	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	7	31	theme	kinetic	1461:1467	arg1	behavior					1469:1476	the kinetic behavior	1457:1476	the kinetic behavior of the process than other models	1457:1509	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	6	32	theme	CMC-g-P	1272:1278	arg1	/MMT					1284:1287	CMC-g-P(AAm)/MMT	1272:1287	CMC-g-P(AAm)/MMT	1272:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	11	33	theme	nanocomposite	2020:2032	arg1	hydrogels					2034:2042	/MMT nanocomposite hydrogels	2015:2042	/MMT nanocomposite hydrogels	2015:2042	In addition, the enthalpy parameter (ΔH°) was determined for the adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT nanocomposite hydrogels at 39.859 KJ/mol and 74.736 KJ/mol, respectively.					
32422256	5	34	with	agreement	1037:1045	arg1	model					1074:1078	the Langmuir isotherm model	1052:1078	the Langmuir isotherm model	1052:1078	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	13	35	theme	ions	2388:2391	arg1	concentration					2393:2405	ions concentration	2388:2405	ions concentration	2388:2405	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	13	36	theme	concentrations	2207:2220	arg1	effect					2222:2227	the concentrations effect	2203:2227	the concentrations effect of Na+ and K+ ions on adsorption efficiency	2203:2271	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	11	37	dep	CMC-g-P	1986:1992	arg1	hydrogels					2034:2042	/MMT nanocomposite hydrogels	2015:2042	/MMT nanocomposite hydrogels	2015:2042	In addition, the enthalpy parameter (ΔH°) was determined for the adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT nanocomposite hydrogels at 39.859 KJ/mol and 74.736 KJ/mol, respectively.					
32422256	2	38	theme	hydrogel	579:586	arg1	system					588:593	the hydrogel system	575:593	the hydrogel system	575:593	The properties and characterization of the adsorbents were investigated using FTIR, SEM, TGA, and XRD analyzes, and the results showed that MMT nanoparticles were successfully distributed in the hydrogel system.					
32422256	1	39	theme	green	338:342	arg1	removal					353:359	malachite green (MG) dye removal	328:359	malachite green (MG) dye removal from aqueous solution	328:381	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	14	40	theme	adsorbent	2532:2540	arg1	surface					2542:2548	the adsorbent surface	2528:2548	the adsorbent surface with the MG dye molecule	2528:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	10	41	from	processes	1815:1823	arg1	range					1832:1836	the range	1828:1836	the range of 25-50 °C	1828:1848	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	12	42	from	range	2156:2160	arg1	endothermic					2137:2147	endothermic	2137:2147	endothermic	2137:2147	Positive ΔH° indicates that the process is endothermic in the range of 25-50 °C using both adsorbents.					
32422256	12	42	from	range	2156:2160	arg1	process					2126:2132	the process	2122:2132	the process	2122:2132	Positive ΔH° indicates that the process is endothermic in the range of 25-50 °C using both adsorbents.					
32422256	0	43	theme	malachite	11:19	arg1	green					21:25	malachite green	11:25	malachite green	11:25	Removal of malachite green using carboxymethyl cellulose-g-polyacrylamide/montmorillonite nanocomposite hydrogel.					
32422256	1	44	with	nanocomposite	214:226	arg1	montmorillonite					233:247	montmorillonite	233:247	montmorillonite (CMC-g-P(AAm)/MMT)	233:266	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	44	with	nanocomposite	214:226	arg1	/MMT					262:265	CMC-g-P(AAm)/MMT	250:265	CMC-g-P(AAm)/MMT	250:265	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	45	theme	cellulose	143:151	arg1	CMC-g-P					192:198	CMC-g-P	192:198	CMC-g-P(AAm)	192:203	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	45	theme	cellulose	143:151	arg1	hydrogel					182:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel	129:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm))	129:204	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	14	46	theme	electrostatic	2498:2510	arg1	interactions					2512:2523	repulsive electrostatic interactions	2488:2523	repulsive electrostatic interactions	2488:2523	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	3	47	theme	MMT	596:598	arg1	nanoparticles					600:612	MMT nanoparticles	596:612	MMT nanoparticles	596:612	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	5	48	theme	Langmuir	1056:1063	arg1	model					1074:1078	the Langmuir isotherm model	1052:1078	the Langmuir isotherm model	1052:1078	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	7	49	theme	order	1407:1411	arg1	model					1421:1425	the pseudo-second- order kinetic model	1388:1425	the pseudo-second- order kinetic model	1388:1425	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	7	49	theme	order	1407:1411	arg1	capable					1435:1441	capable	1435:1441	capable	1435:1441	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	5	50	theme	Equilibrium	920:930	arg1	investigation					941:953	Equilibrium behavior investigation	920:953	Equilibrium behavior investigation of the adsorption process	920:979	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	13	51	theme	process	2349:2355	arg1	efficiency					2320:2329	the efficiency	2316:2329	the efficiency of the adsorption process	2316:2355	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	1	52	theme	radical	294:300	arg1	method					302:307	the free radical method	285:307	the free radical method	285:307	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	14	53	theme	active	2471:2476	arg1	sites					2478:2482	active sites	2471:2482	active sites	2471:2482	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	8	54	theme	parameter	1524:1532	arg1	values					1534:1539	the α parameter values	1518:1539	the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1518:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	8	54	theme	parameter	1524:1532	arg1	0.6337 mg/g·min					1637:1651	0.6337 mg/g·min	1637:1651	0.6337 mg/g·min	1637:1651	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	7	55	theme	kinetic	1362:1368	arg1	study					1370:1374	the kinetic study	1358:1374	the kinetic study	1358:1374	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	5	56	theme	isotherm	1065:1072	arg1	model					1074:1078	the Langmuir isotherm model	1052:1078	the Langmuir isotherm model	1052:1078	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	10	57	theme	free	1765:1768	arg1	energy					1770:1775	Gibbs free energy	1759:1775	Gibbs free energy (ΔG°)	1759:1781	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	10	57	theme	free	1765:1768	arg1	ΔG°					1778:1780	ΔG°	1778:1780	ΔG°	1778:1780	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	3	58	theme	hydrogel	635:642	arg1	system					644:649	the hydrogel system	631:649	the hydrogel system	631:649	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	8	59	theme	MG	1549:1550	arg1	process					1567:1573	the MG dye adsorption process	1545:1573	the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1545:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	4	60	theme	time	804:807	arg1	effect					773:778	the effect	769:778	the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye	769:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	9	61	theme	adsorption	1741:1750	arg1	value					1752:1756	a high adsorption value	1734:1756	a high adsorption value	1734:1756	This indicates that the produced composite has a high adsorption value.					
32422256	6	62	theme	adsorption	1179:1188	arg1	158.1 mg/g					1311:1320	158.1 mg/g	1311:1320	158.1 mg/g	1311:1320	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	6	62	theme	adsorption	1179:1188	arg1	qmax					1200:1203	qmax	1200:1203	qmax	1200:1203	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	6	62	theme	adsorption	1179:1188	arg1	capacity					1190:1197	The maximum monolayer adsorption capacity	1157:1197	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1157:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	8	63	theme	adsorption	1556:1565	arg1	process					1567:1573	the MG dye adsorption process	1545:1573	the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1545:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	3	64	theme	weight	666:671	arg1	percentages					673:683	different weight percentages	656:683	different weight percentages	656:683	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	6	65	theme	maximum	1161:1167	arg1	158.1 mg/g					1311:1320	158.1 mg/g	1311:1320	158.1 mg/g	1311:1320	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	6	65	theme	maximum	1161:1167	arg1	qmax					1200:1203	qmax	1200:1203	qmax	1200:1203	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	6	65	theme	maximum	1161:1167	arg1	capacity					1190:1197	The maximum monolayer adsorption capacity	1157:1197	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1157:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	14	66	theme	efficiency	2419:2428	arg1	decrease					2430:2437	Adsorption efficiency decrease	2408:2437	Adsorption efficiency decrease	2408:2437	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	4	67	theme	dye	838:840	arg1	temperature					783:793	temperature	783:793	temperature	783:793	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	4	67	theme	dye	838:840	arg1	time					804:807	contact time	796:807	contact time	796:807	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	4	67	theme	dye	838:840	arg1	concentration					818:830	initial concentration	810:830	initial concentration of MG dye	810:840	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	4	67	theme	dye	838:840	arg1	pH					854:855	initial pH	846:855	initial pH	846:855	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	3	68	theme	maximum	693:699	arg1	efficiency					712:721	the maximum adsorption efficiency	689:721	the maximum adsorption efficiency of MG dye	689:731	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	1	69	used	used	320:323	arg2	it					313:314	it	313:314	it	313:314	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	4	70	theme	initial	846:852	arg1	pH					854:855	initial pH	846:855	initial pH	846:855	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	6	71	theme	Langmuir	1227:1234	arg1	model					1245:1249	the Langmuir isotherm model	1223:1249	the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1223:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	5	72	theme	equilibrium	997:1007	arg1	data					1009:1012	the equilibrium data	993:1012	the equilibrium data determined	993:1023	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	8	73	theme	CMC-g-P	1598:1604	arg1	/MMT					1610:1613	CMC-g-P(AAm)/MMT	1598:1613	CMC-g-P(AAm)/MMT	1598:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	1	74	theme	based	153:157	arg1	CMC-g-P					192:198	CMC-g-P	192:198	CMC-g-P(AAm)	192:203	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	74	theme	based	153:157	arg1	hydrogel					182:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel	129:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm))	129:204	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	4	75	theme	initial	810:816	arg1	concentration					818:830	initial concentration	810:830	initial concentration of MG dye	810:840	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	12	76	theme	Positive	2094:2101	arg1	ΔH°					2103:2105	Positive ΔH°	2094:2105	Positive ΔH°	2094:2105	Positive ΔH° indicates that the process is endothermic in the range of 25-50 °C using both adsorbents.					
32422256	3	77	theme	MG	726:727	arg1	dye					729:731	MG dye	726:731	MG dye	726:731	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	13	78	theme	K+	2240:2241	arg1	ions					2243:2246	Na+ and K+ ions	2232:2246	Na+ and K+ ions	2232:2246	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	1	79	theme	aqueous	366:372	arg1	solution					374:381	aqueous solution	366:381	aqueous solution	366:381	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	8	80	theme	AAm	1606:1608	arg1	/MMT					1610:1613	CMC-g-P(AAm)/MMT	1598:1613	CMC-g-P(AAm)/MMT	1598:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	1	81	theme	poly	165:168	arg1	CMC-g-P					192:198	CMC-g-P	192:198	CMC-g-P(AAm)	192:203	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	81	theme	poly	165:168	arg1	hydrogel					182:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel	129:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm))	129:204	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	14	82	theme	MG	2559:2560	arg1	molecule					2566:2573	the MG dye molecule	2555:2573	the MG dye molecule	2555:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	3	83	theme	%	756:756	arg1	MMT					758:760	10 wt% MMT	751:760	10 wt% MMT	751:760	MMT nanoparticles were loaded into the hydrogel system with different weight percentages and the maximum adsorption efficiency of MG dye was determined at 10 wt% MMT.					
32422256	1	84	from	solution	374:381	arg1	removal					353:359	malachite green (MG) dye removal	328:359	malachite green (MG) dye removal from aqueous solution	328:381	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	5	85	theme	process	973:979	arg1	investigation					941:953	Equilibrium behavior investigation	920:953	Equilibrium behavior investigation of the adsorption process	920:979	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	1	86	theme	acrylamide	170:179	arg1	CMC-g-P					192:198	CMC-g-P	192:198	CMC-g-P(AAm)	192:203	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	86	theme	acrylamide	170:179	arg1	hydrogel					182:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel	129:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm))	129:204	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	13	87	from	effect	2222:2227	arg1	efficiency					2262:2271	adsorption efficiency	2251:2271	adsorption efficiency	2251:2271	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	14	88	with	surface	2542:2548	arg1	molecule					2566:2573	the MG dye molecule	2555:2573	the MG dye molecule	2555:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	1	89	with	hydrogel	182:189	arg1	montmorillonite					233:247	montmorillonite	233:247	montmorillonite (CMC-g-P(AAm)/MMT)	233:266	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	89	with	hydrogel	182:189	arg1	/MMT					262:265	CMC-g-P(AAm)/MMT	250:265	CMC-g-P(AAm)/MMT	250:265	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	4	90	theme	dye	892:894	arg1	efficiency					875:884	the adsorption efficiency	860:884	the adsorption efficiency of MG dye	860:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	13	91	theme	concentration	2393:2405	arg1	increase					2376:2383	the increase	2372:2383	the increase of ions concentration	2372:2405	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	1	92	theme	malachite	328:336	arg1	MG					345:346	MG	345:346	MG	345:346	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	92	theme	malachite	328:336	arg1	green					338:342	malachite green	328:342	malachite green (MG) dye removal from aqueous solution	328:381	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	2	93	dep	properties	388:397	arg1	The					384:386	The	384:386	The	384:386	The properties and characterization of the adsorbents were investigated using FTIR, SEM, TGA, and XRD analyzes, and the results showed that MMT nanoparticles were successfully distributed in the hydrogel system.					
32422256	4	94	theme	adsorption	864:873	arg1	efficiency					875:884	the adsorption efficiency	860:884	the adsorption efficiency of MG dye	860:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	14	95	from	surface	2542:2548	arg1	occupation					2457:2466	the occupation	2453:2466	the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule	2453:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	2	96	theme	XRD	482:484	arg1	analyzes					486:493	XRD analyzes	482:493	XRD analyzes	482:493	The properties and characterization of the adsorbents were investigated using FTIR, SEM, TGA, and XRD analyzes, and the results showed that MMT nanoparticles were successfully distributed in the hydrogel system.					
32422256	0	97	theme	green	21:25	arg1	Removal					0:6	Removal	0:6	Removal of malachite green using carboxymethyl cellulose-g-polyacrylamide/montmorillonite	0:88	Removal of malachite green using carboxymethyl cellulose-g-polyacrylamide/montmorillonite nanocomposite hydrogel.					
32422256	5	98	theme	monolayer	1088:1096	arg1	surfaces					1098:1105	the monolayer surfaces	1084:1105	the monolayer surfaces	1084:1105	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	1	99	theme	carboxymethyl	129:141	arg1	CMC-g-P					192:198	CMC-g-P	192:198	CMC-g-P(AAm)	192:203	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	99	theme	carboxymethyl	129:141	arg1	hydrogel					182:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel	129:189	carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm))	129:204	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	14	100	theme	interactions	2512:2523	arg1	occupation					2457:2466	the occupation	2453:2466	the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule	2453:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	11	101	theme	adsorption	1961:1970	arg1	process					1972:1978	the adsorption process	1957:1978	the adsorption process	1957:1978	In addition, the enthalpy parameter (ΔH°) was determined for the adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT nanocomposite hydrogels at 39.859 KJ/mol and 74.736 KJ/mol, respectively.					
32422256	0	102	theme	carboxymethyl	33:45	arg1	cellulose-g-polyacrylamide/montmorillonite					47:88	carboxymethyl cellulose-g-polyacrylamide/montmorillonite	33:88	carboxymethyl cellulose-g-polyacrylamide/montmorillonite	33:88	Removal of malachite green using carboxymethyl cellulose-g-polyacrylamide/montmorillonite nanocomposite hydrogel.					
32422256	14	103	theme	repulsive	2488:2496	arg1	interactions					2512:2523	repulsive electrostatic interactions	2488:2523	repulsive electrostatic interactions	2488:2523	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	2	104	theme	adsorbents	427:436	arg1	characterization					403:418	characterization	403:418	characterization	403:418	The properties and characterization of the adsorbents were investigated using FTIR, SEM, TGA, and XRD analyzes, and the results showed that MMT nanoparticles were successfully distributed in the hydrogel system.					
32422256	2	104	theme	adsorbents	427:436	arg1	properties					388:397	properties	388:397	properties	388:397	The properties and characterization of the adsorbents were investigated using FTIR, SEM, TGA, and XRD analyzes, and the results showed that MMT nanoparticles were successfully distributed in the hydrogel system.					
32422256	11	105	theme	/MMT	2015:2018	arg1	hydrogels					2034:2042	/MMT nanocomposite hydrogels	2015:2042	/MMT nanocomposite hydrogels	2015:2042	In addition, the enthalpy parameter (ΔH°) was determined for the adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT nanocomposite hydrogels at 39.859 KJ/mol and 74.736 KJ/mol, respectively.					
32422256	13	106	theme	adsorption	2338:2347	arg1	process					2349:2355	the adsorption process	2334:2355	the adsorption process	2334:2355	Also, the concentrations effect of Na+ and K+ ions on adsorption efficiency was investigated and it was concluded that the efficiency of the adsorption process decreased with the increase of ions concentration.					
32422256	1	107	theme	free	289:292	arg1	radical					294:300	the free radical	285:300	the free radical method	285:307	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	11	108	theme	enthalpy	1913:1920	arg1	ΔH°					1933:1935	ΔH°	1933:1935	ΔH°	1933:1935	In addition, the enthalpy parameter (ΔH°) was determined for the adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT nanocomposite hydrogels at 39.859 KJ/mol and 74.736 KJ/mol, respectively.					
32422256	11	108	theme	enthalpy	1913:1920	arg1	parameter					1922:1930	the enthalpy parameter	1909:1930	the enthalpy parameter (ΔH°)	1909:1936	In addition, the enthalpy parameter (ΔH°) was determined for the adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT nanocomposite hydrogels at 39.859 KJ/mol and 74.736 KJ/mol, respectively.					
32422256	7	109	theme	kinetic	1413:1419	arg1	model					1421:1425	the pseudo-second- order kinetic model	1388:1425	the pseudo-second- order kinetic model	1388:1425	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	7	109	theme	kinetic	1413:1419	arg1	capable					1435:1441	capable	1435:1441	capable	1435:1441	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	5	110	theme	behavior	932:939	arg1	investigation					941:953	Equilibrium behavior investigation	920:953	Equilibrium behavior investigation of the adsorption process	920:979	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	4	111	from	effect	773:778	arg1	efficiency					875:884	the adsorption efficiency	860:884	the adsorption efficiency of MG dye	860:894	Also, the effect of temperature, contact time, initial concentration of MG dye and initial pH on the adsorption efficiency of MG dye was studied in a batch.					
32422256	14	112	theme	sites	2478:2482	arg1	occupation					2457:2466	the occupation	2453:2466	the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule	2453:2573	Adsorption efficiency decrease can be due to the occupation of active sites and repulsive electrostatic interactions at the adsorbent surface with the MG dye molecule.					
32422256	6	113	theme	isotherm	1236:1243	arg1	model					1245:1249	the Langmuir isotherm model	1223:1249	the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1223:1287	The maximum monolayer adsorption capacity (qmax) determined using the Langmuir isotherm model for CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 158.1 mg/g and 172.4 mg/g, respectively.					
32422256	8	114	theme	α	1522:1522	arg1	values					1534:1539	the α parameter values	1518:1539	the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT	1518:1613	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	8	114	theme	α	1522:1522	arg1	0.6337 mg/g·min					1637:1651	0.6337 mg/g·min	1637:1651	0.6337 mg/g·min	1637:1651	Also, the α parameter values for the MG dye adsorption process using CMC-g-P(AAm) and CMC-g-P(AAm)/MMT were determined to be 0.6337 mg/g·min and 31.04 mg/g·min, respectively.					
32422256	7	115	theme	pseudo-second-	1392:1405	arg1	model					1421:1425	the pseudo-second- order kinetic model	1388:1425	the pseudo-second- order kinetic model	1388:1425	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	7	115	theme	pseudo-second-	1392:1405	arg1	capable					1435:1441	capable	1435:1441	capable	1435:1441	Also, the kinetic study showed that the pseudo-second- order kinetic model is more capable of describing the kinetic behavior of the process than other models.					
32422256	12	116	theme	25-50 °C	2165:2172	arg1	range					2156:2160	the range	2152:2160	the range of 25-50 °C using both adsorbents	2152:2194	Positive ΔH° indicates that the process is endothermic in the range of 25-50 °C using both adsorbents.					
32422256	9	117	contain	has	1730:1732	arg2	value					1752:1756	a high adsorption value	1734:1756	a high adsorption value	1734:1756	This indicates that the produced composite has a high adsorption value.					
32422256	9	117	contain	has	1730:1732	arg1	composite					1720:1728	the produced composite	1707:1728	the produced composite	1707:1728	This indicates that the produced composite has a high adsorption value.					
32422256	5	118	theme	adsorption	1137:1146	arg1	process					1148:1154	the adsorption process	1133:1154	the adsorption process	1133:1154	Equilibrium behavior investigation of the adsorption process showed that the equilibrium data determined are in good agreement with the Langmuir isotherm model and the monolayer surfaces play an effective role in the adsorption process.					
32422256	9	119	theme	produced	1711:1718	arg1	composite					1720:1728	the produced composite	1707:1728	the produced composite	1707:1728	This indicates that the produced composite has a high adsorption value.					
32422256	10	120	theme	Gibbs	1759:1763	arg1	energy					1770:1775	Gibbs free energy	1759:1775	Gibbs free energy (ΔG°)	1759:1781	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	10	120	theme	Gibbs	1759:1763	arg1	ΔG°					1778:1780	ΔG°	1778:1780	ΔG°	1778:1780	Gibbs free energy (ΔG°) was negative for the adsorption processes in the range of 25-50 °C, indicating that the process was spontaneous.					
32422256	1	121	theme	CMC-g-P	250:256	arg1	montmorillonite					233:247	montmorillonite	233:247	montmorillonite (CMC-g-P(AAm)/MMT)	233:266	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
32422256	1	121	theme	CMC-g-P	250:256	arg1	/MMT					262:265	CMC-g-P(AAm)/MMT	250:265	CMC-g-P(AAm)/MMT	250:265	In this study, carboxymethyl cellulose based graft poly(acrylamide) hydrogel (CMC-g-P(AAm)) and its nanocomposite with montmorillonite (CMC-g-P(AAm)/MMT) were produced by the free radical method and it was used to malachite green (MG) dye removal from aqueous solution.					
33182135	8	0	theme	horseradish	1571:1581	arg1	system					1609:1614	the co-immobilized horseradish peroxidase-syringaldehyde system	1552:1614	the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst	1552:1629	This research demonstrated and provided a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst.					
33182135	3	1	theme	co-immobilized	557:570	arg1	system					610:615	the co-immobilized horseradish peroxidase-syringaldehyde system	553:615	the co-immobilized horseradish peroxidase-syringaldehyde system	553:615	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	1	2	theme	environmental	267:279	arg1	friendliness					281:292	environmental friendliness	267:292	environmental friendliness	267:292	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	0	3	theme	horseradish	73:83	arg1	peroxidase-syringaldehyde					85:109	co-immobilized horseradish peroxidase-syringaldehyde	58:109	co-immobilized horseradish peroxidase-syringaldehyde	58:109	A novel approach to efficient degradation of indole using co-immobilized horseradish peroxidase-syringaldehyde as biocatalyst.					
33182135	1	4	from	deal	184:187	arg1	treatment					211:219	water treatment	205:219	water treatment	205:219	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	3	5	theme	horseradish	572:582	arg1	system					610:615	the co-immobilized horseradish peroxidase-syringaldehyde system	553:615	the co-immobilized horseradish peroxidase-syringaldehyde system	553:615	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	5	6	from	25 mg/L	1022:1028	arg1	low					1015:1017	low	1015:1017	low	1015:1017	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	5	6	from	25 mg/L	1022:1028	arg1	concentration					988:1000	the concentration	984:1000	the concentration of indole	984:1010	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	1	7	theme	friendliness	281:292	arg1	advantages					236:245	its advantages	232:245	its advantages of high efficiency, environmental friendliness, and no secondary pollution	232:320	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	6	8	theme	horseradish	1151:1161	arg1	peroxidase					1163:1172	horseradish peroxidase	1151:1172	horseradish peroxidase	1151:1172	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	3	9	theme	excellent	631:639	arg1	performance					651:661	excellent catalytic performance	631:661	excellent catalytic performance	631:661	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	7	10	theme	spherical	1278:1286	arg1	catalyst					1288:1295	the spherical catalyst	1274:1295	the spherical catalyst	1274:1295	Furthermore, while the spherical catalyst was operated in succession and reused for four cycles in 50 mg/L indole solution, the degradation rate remained 91.8% due to its considerable reusability.					
33182135	3	11	theme	catalytic	641:649	arg1	performance					651:661	excellent catalytic performance	631:661	excellent catalytic performance	631:661	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	2	12	theme	first	339:343	arg1	time					345:348	the first time	335:348	the first time	335:348	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	2	13	theme	horseradish	351:361	arg1	peroxidase					363:372	horseradish peroxidase	351:372	horseradish peroxidase	351:372	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	4	14	theme	%	718:718	arg1	rate					707:710	The degradation rate	691:710	The degradation rate of 100%	691:718	The degradation rate of 100% was achieved in the presence of hydrogen peroxide even if the indole concentration was changing from 25 mg/L to 500 mg/L.					
33182135	4	15	theme	hydrogen	752:759	arg1	peroxide					761:768	hydrogen peroxide	752:768	hydrogen peroxide	752:768	The degradation rate of 100% was achieved in the presence of hydrogen peroxide even if the indole concentration was changing from 25 mg/L to 500 mg/L.					
33182135	1	16	theme	attention	192:200	arg1	deal					184:187	a great deal	176:187	a great deal of attention in water treatment	176:219	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	6	17	theme	effective	1058:1066	arg1	co-immobilization					1068:1084	the effective co-immobilization	1054:1084	the effective co-immobilization of the enzyme	1054:1098	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	4	18	theme	degradation	695:705	arg1	rate					707:710	The degradation rate	691:710	The degradation rate of 100%	691:718	The degradation rate of 100% was achieved in the presence of hydrogen peroxide even if the indole concentration was changing from 25 mg/L to 500 mg/L.					
33182135	6	19	theme	catalytic	1129:1137	arg1	activity					1139:1146	the catalytic activity	1125:1146	the catalytic activity of horseradish peroxidase	1125:1172	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	0	20	theme	novel	2:6	arg1	approach					8:15	A novel approach	0:15	A novel approach to efficient degradation of indole	0:50	A novel approach to efficient degradation of indole using co-immobilized horseradish peroxidase-syringaldehyde as biocatalyst.					
33182135	3	21	theme	system	610:615	arg1	biocatalyst					538:548	The resultant biocatalyst	524:548	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system	524:615	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	7	22	from	cycles	1344:1349	arg1	solution					1369:1376	50 mg/L indole solution	1354:1376	50 mg/L indole solution	1354:1376	Furthermore, while the spherical catalyst was operated in succession and reused for four cycles in 50 mg/L indole solution, the degradation rate remained 91.8% due to its considerable reusability.					
33182135	5	23	theme	indole	1005:1010	arg1	low					1015:1017	low	1015:1017	low	1015:1017	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	5	23	theme	indole	1005:1010	arg1	concentration					988:1000	the concentration	984:1000	the concentration of indole	984:1010	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	1	24	theme	water	205:209	arg1	treatment					211:219	water treatment	205:219	water treatment	205:219	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	2	25	theme	glycidyl	488:495	arg1	methacrylate					497:508	glycidyl methacrylate	488:508	glycidyl methacrylate	488:508	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	5	26	theme	identical	885:893	arg1	conditions					911:920	the identical water treatment conditions	881:920	the identical water treatment conditions	881:920	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	1	27	theme	secondary	302:310	arg1	pollution					312:320	no secondary pollution	299:320	no secondary pollution	299:320	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	5	28	used	used	870:873	arg2	enzyme					859:864	only the free enzyme	845:864	only the free enzyme	845:864	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	6	29	theme	syringaldehyde	1214:1227	arg1	action					1204:1209	the synergistic catalytic action	1178:1209	the synergistic catalytic action of syringaldehyde	1178:1227	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	6	29	theme	syringaldehyde	1214:1227	arg1	activity					1139:1146	the catalytic activity	1125:1146	the catalytic activity of horseradish peroxidase	1125:1172	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	5	30	theme	treatment	901:909	arg1	conditions					911:920	the identical water treatment conditions	881:920	the identical water treatment conditions	881:920	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	5	31	theme	water	895:899	arg1	conditions					911:920	the identical water treatment conditions	881:920	the identical water treatment conditions	881:920	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	1	32	theme	pollution	312:320	arg1	advantages					236:245	its advantages	232:245	its advantages of high efficiency, environmental friendliness, and no secondary pollution	232:320	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	1	33	theme	Biocatalytic	127:138	arg1	technology					152:161	Biocatalytic degradation technology	127:161	Biocatalytic degradation technology	127:161	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	2	34	theme	composite	459:467	arg1	beads					469:473	functionalized calcium alginate composite beads	427:473	functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine	427:521	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	5	35	theme	free	854:857	arg1	enzyme					859:864	only the free enzyme	845:864	only the free enzyme	845:864	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	6	36	theme	catalytic	1194:1202	arg1	action					1204:1209	the synergistic catalytic action	1178:1209	the synergistic catalytic action of syringaldehyde	1178:1227	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	7	37	theme	indole	1362:1367	arg1	solution					1369:1376	50 mg/L indole solution	1354:1376	50 mg/L indole solution	1354:1376	Furthermore, while the spherical catalyst was operated in succession and reused for four cycles in 50 mg/L indole solution, the degradation rate remained 91.8% due to its considerable reusability.					
33182135	2	38	theme	alginate	450:457	arg1	beads					469:473	functionalized calcium alginate composite beads	427:473	functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine	427:521	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	0	39	theme	efficient	20:28	arg1	degradation					30:40	efficient degradation	20:40	efficient degradation of indole	20:50	A novel approach to efficient degradation of indole using co-immobilized horseradish peroxidase-syringaldehyde as biocatalyst.					
33182135	5	40	theme	indole	942:947	arg1	degradation					927:937	the degradation	923:937	the degradation of indole	923:947	If only the free enzyme was used under the identical water treatment conditions, the degradation of indole could hardly be observed even when the concentration of indole is low at 25 mg/L.					
33182135	2	41	theme	calcium	442:448	arg1	beads					469:473	functionalized calcium alginate composite beads	427:473	functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine	427:521	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	0	42	theme	indole	45:50	arg1	degradation					30:40	efficient degradation	20:40	efficient degradation of indole	20:50	A novel approach to efficient degradation of indole using co-immobilized horseradish peroxidase-syringaldehyde as biocatalyst.					
33182135	7	43	theme	considerable	1426:1437	arg1	reusability					1439:1449	its considerable reusability	1422:1449	its considerable reusability	1422:1449	Furthermore, while the spherical catalyst was operated in succession and reused for four cycles in 50 mg/L indole solution, the degradation rate remained 91.8% due to its considerable reusability.					
33182135	6	44	theme	synergistic	1182:1192	arg1	action					1204:1209	the synergistic catalytic action	1178:1209	the synergistic catalytic action of syringaldehyde	1178:1227	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	1	45	theme	degradation	140:150	arg1	technology					152:161	Biocatalytic degradation technology	127:161	Biocatalytic degradation technology	127:161	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	2	46	theme	functionalized	427:440	arg1	beads					469:473	functionalized calcium alginate composite beads	427:473	functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine	427:521	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	4	47	theme	indole	782:787	arg1	concentration					789:801	the indole concentration	778:801	the indole concentration	778:801	The degradation rate of 100% was achieved in the presence of hydrogen peroxide even if the indole concentration was changing from 25 mg/L to 500 mg/L.					
33182135	8	48	theme	biocatalytic	1500:1511	arg1	approach					1513:1520	a novel biocatalytic approach	1492:1520	a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst	1492:1629	This research demonstrated and provided a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst.					
33182135	8	49	theme	novel	1494:1498	arg1	approach					1513:1520	a novel biocatalytic approach	1492:1520	a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst	1492:1629	This research demonstrated and provided a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst.					
33182135	4	50	theme	peroxide	761:768	arg1	presence					740:747	the presence	736:747	the presence of hydrogen peroxide	736:768	The degradation rate of 100% was achieved in the presence of hydrogen peroxide even if the indole concentration was changing from 25 mg/L to 500 mg/L.					
33182135	3	51	theme	resultant	528:536	arg1	biocatalyst					538:548	The resultant biocatalyst	524:548	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system	524:615	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	6	52	theme	enzyme	1093:1098	arg1	mediator					1108:1115	the mediator	1104:1115	the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed	1104:1252	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	6	52	theme	enzyme	1093:1098	arg1	co-immobilization					1068:1084	the effective co-immobilization	1054:1084	the effective co-immobilization of the enzyme	1054:1098	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	8	53	theme	co-immobilized	1556:1569	arg1	system					1609:1614	the co-immobilized horseradish peroxidase-syringaldehyde system	1552:1614	the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst	1552:1629	This research demonstrated and provided a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst.					
33182135	2	54	theme	mediator	378:385	arg1	syringaldehyde					387:400	mediator syringaldehyde	378:400	mediator syringaldehyde	378:400	Herein, for the first time, horseradish peroxidase and mediator syringaldehyde were co-immobilized into functionalized calcium alginate composite beads grafted with glycidyl methacrylate and dopamine.					
33182135	7	55	theme	degradation	1383:1393	arg1	rate					1395:1398	the degradation rate	1379:1398	the degradation rate	1379:1398	Furthermore, while the spherical catalyst was operated in succession and reused for four cycles in 50 mg/L indole solution, the degradation rate remained 91.8% due to its considerable reusability.					
33182135	1	56	theme	high	250:253	arg1	efficiency					255:264	high efficiency	250:264	high efficiency	250:264	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	0	57	theme	co-immobilized	58:71	arg1	peroxidase-syringaldehyde					85:109	co-immobilized horseradish peroxidase-syringaldehyde	58:109	co-immobilized horseradish peroxidase-syringaldehyde	58:109	A novel approach to efficient degradation of indole using co-immobilized horseradish peroxidase-syringaldehyde as biocatalyst.					
33182135	6	58	theme	peroxidase	1163:1172	arg1	action					1204:1209	the synergistic catalytic action	1178:1209	the synergistic catalytic action of syringaldehyde	1178:1227	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	6	58	theme	peroxidase	1163:1172	arg1	activity					1139:1146	the catalytic activity	1125:1146	the catalytic activity of horseradish peroxidase	1125:1172	This was attributed to the effective co-immobilization of the enzyme and the mediator so that the catalytic activity of horseradish peroxidase and the synergistic catalytic action of syringaldehyde could be fully developed.					
33182135	3	59	theme	peroxidase-syringaldehyde	584:608	arg1	system					610:615	the co-immobilized horseradish peroxidase-syringaldehyde system	553:615	the co-immobilized horseradish peroxidase-syringaldehyde system	553:615	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	3	60	from	indole	674:679	arg1	water					684:688	water	684:688	water	684:688	The resultant biocatalyst of the co-immobilized horseradish peroxidase-syringaldehyde system has displayed excellent catalytic performance to degrade indole in water.					
33182135	1	61	theme	efficiency	255:264	arg1	advantages					236:245	its advantages	232:245	its advantages of high efficiency, environmental friendliness, and no secondary pollution	232:320	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
33182135	8	62	from	indole	1533:1538	arg1	water					1543:1547	water	1543:1547	water	1543:1547	This research demonstrated and provided a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst.					
33182135	8	63	theme	peroxidase-syringaldehyde	1583:1607	arg1	system					1609:1614	the co-immobilized horseradish peroxidase-syringaldehyde system	1552:1614	the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst	1552:1629	This research demonstrated and provided a novel biocatalytic approach to degrade indole in water by the co-immobilized horseradish peroxidase-syringaldehyde system as biocatalyst.					
33182135	7	64	theme	50 mg/L	1354:1360	arg1	solution					1369:1376	50 mg/L indole solution	1354:1376	50 mg/L indole solution	1354:1376	Furthermore, while the spherical catalyst was operated in succession and reused for four cycles in 50 mg/L indole solution, the degradation rate remained 91.8% due to its considerable reusability.					
33182135	1	65	theme	great	178:182	arg1	deal					184:187	a great deal	176:187	a great deal of attention in water treatment	176:219	Biocatalytic degradation technology has received a great deal of attention in water treatment because of its advantages of high efficiency, environmental friendliness, and no secondary pollution.					
32381549	0	0	theme	Cartilage	97:105	arg1	Development					107:117	Cartilage Development	97:117	Cartilage Development	97:117	Perlecan Knockdown Significantly Alters Extracellular Matrix Composition and Organization During Cartilage Development.					
32381549	3	1	theme	many	877:880	arg1	collagens					882:890	many collagens	877:890	many collagens	877:890	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	2	theme	bulk	848:851	arg1	collagens					882:890	many collagens	877:890	many collagens	877:890	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	2	theme	bulk	848:851	arg1	proteins					857:864	bulk ECM proteins	848:864	bulk ECM proteins	848:864	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	6	3	theme	osteoarthritis	1413:1426	arg1	assembly					1381:1388	functional cartilage assembly	1360:1388	functional cartilage assembly	1360:1388	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	6	3	theme	osteoarthritis	1413:1426	arg1	progression					1398:1408	the progression	1394:1408	the progression of osteoarthritis in SJS	1394:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	1	4	located	found	156:160	arg1	ECM					191:193	ECM	191:193	ECM	191:193	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	1	4	located	found	156:160	arg1	matrix					183:188	the extracellular matrix	165:188	the extracellular matrix (ECM) of cartilage	165:207	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	1	4	located	found	156:160	arg2	Perlecan					120:127	Perlecan	120:127	Perlecan	120:127	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	1	4	located	found	156:160	arg2	proteoglycan					143:154	a critical proteoglycan	132:154	a critical proteoglycan found in the extracellular matrix (ECM) of cartilage	132:207	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	4	5	theme	LC-MS/MS	1012:1019	arg1	analysis					1021:1028	LC-MS/MS analysis	1012:1028	LC-MS/MS analysis	1012:1028	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	6	6	from	progression	1398:1408	arg1	SJS					1431:1433	SJS	1431:1433	SJS	1431:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	4	7	dep	showed	1030:1035	arg1	secreted					1054:1061	secreted	1054:1061	secreted by Hspg2 cartilage in vitro	1054:1089	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	5	8	theme	observed	1254:1261	arg1	decrease					1263:1270	the previously observed decrease	1239:1270	the previously observed decrease in mechanics	1239:1283	In addition, glycosaminoglycan deposition was atypical, which may explain the previously observed decrease in mechanics.					
32381549	3	9	theme	SJS	745:747	arg1	model					736:740	a murine model	727:740	a murine model of SJS	727:747	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	2	10	theme	perlecan	305:312	arg1	deficiency					314:323	perlecan deficiency	305:323	perlecan deficiency	305:323	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	3	11	theme	murine	729:734	arg1	model					736:740	a murine model	727:740	a murine model of SJS	727:747	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	2	12	from	change	382:387	arg1	mechanics					392:400	mechanics	392:400	mechanics	392:400	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	3	13	theme	perlecan	553:560	arg1	knockdown					562:570	perlecan knockdown	553:570	perlecan knockdown	553:570	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	2	14	theme	Schwartz-Jampel	505:519	arg1	SJS					531:533	SJS	531:533	SJS	531:533	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	2	14	theme	Schwartz-Jampel	505:519	arg1	syndrome					521:528	Schwartz-Jampel syndrome	505:528	Schwartz-Jampel syndrome (SJS)	505:534	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	5	15	from	atypical	1211:1218	arg1	addition					1168:1175	addition	1168:1175	addition	1168:1175	In addition, glycosaminoglycan deposition was atypical, which may explain the previously observed decrease in mechanics.					
32381549	0	16	theme	Perlecan	0:7	arg1	Knockdown					9:17	Perlecan Knockdown	0:17	Perlecan Knockdown	0:17	Perlecan Knockdown Significantly Alters Extracellular Matrix Composition and Organization During Cartilage Development.					
32381549	3	17	theme	Perlecan	756:763	arg1	knockdown					765:773	Hspg2 Perlecan knockdown	750:773	Hspg2 Perlecan knockdown	750:773	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	6	18	from	assembly	1381:1388	arg1	SJS					1431:1433	SJS	1431:1433	SJS	1431:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	4	19	theme	synthesized	1135:1145	arg1	ECM					1147:1149	newly synthesized ECM	1129:1149	newly synthesized ECM	1129:1149	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	1	20	theme	cartilage	199:207	arg1	ECM					191:193	ECM	191:193	ECM	191:193	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	1	20	theme	cartilage	199:207	arg1	matrix					183:188	the extracellular matrix	165:188	the extracellular matrix (ECM) of cartilage	165:207	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	4	21	theme	Hspg2	1066:1070	arg1	cartilage					1072:1080	Hspg2 cartilage	1066:1080	Hspg2 cartilage	1066:1080	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	5	22	from	addition	1168:1175	arg1	atypical					1211:1218	atypical	1211:1218	atypical	1211:1218	In addition, glycosaminoglycan deposition was atypical, which may explain the previously observed decrease in mechanics.					
32381549	3	23	theme	material	584:591	arg1	properties					593:602	the material properties	580:602	the material properties of developing cartilage	580:626	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	0	24	theme	Matrix	54:59	arg1	Composition					61:71	Extracellular Matrix Composition	40:71	Extracellular Matrix Composition	40:71	Perlecan Knockdown Significantly Alters Extracellular Matrix Composition and Organization During Cartilage Development.					
32381549	3	25	theme	Hspg2	750:754	arg1	knockdown					765:773	Hspg2 Perlecan knockdown	750:773	Hspg2 Perlecan knockdown	750:773	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	6	26	theme	cartilage	1371:1379	arg1	assembly					1381:1388	functional cartilage assembly	1360:1388	functional cartilage assembly	1360:1388	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	2	27	theme	cartilage	251:259	arg1	biomechanics					261:272	cartilage biomechanics	251:272	cartilage biomechanics	251:272	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	4	28	theme	ECM	1147:1149	arg1	incorporation					1112:1124	the incorporation	1108:1124	the incorporation of newly synthesized ECM	1108:1149	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	2	29	theme	onset	424:428	arg1	osteoarthritis					430:443	the early onset osteoarthritis	414:443	the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS)	414:534	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	0	30	theme	Extracellular	40:52	arg1	Composition					61:71	Extracellular Matrix Composition	40:71	Extracellular Matrix Composition	40:71	Perlecan Knockdown Significantly Alters Extracellular Matrix Composition and Organization During Cartilage Development.					
32381549	3	31	theme	defective	782:790	arg1	formation					812:820	defective pericellular matrix formation	782:820	defective pericellular matrix formation	782:820	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	4	32	theme	collagens	959:967	arg1	modifications					923:935	Post-translational modifications	904:935	Post-translational modifications	904:935	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	4	32	theme	collagens	959:967	arg1	ultrastructure					941:954	ultrastructure	941:954	ultrastructure of collagens	941:967	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	3	33	theme	chromatography-tandem	656:676	arg1	spectrometry					683:694	liquid chromatography-tandem mass spectrometry	649:694	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	649:705	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	33	theme	chromatography-tandem	656:676	arg1	LC-MS/MS					697:704	LC-MS/MS	697:704	LC-MS/MS	697:704	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	2	34	theme	early	418:422	arg1	osteoarthritis					430:443	the early onset osteoarthritis	414:443	the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS)	414:534	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	3	35	theme	pericellular	792:803	arg1	formation					812:820	defective pericellular matrix formation	782:820	defective pericellular matrix formation	782:820	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	4	36	theme	Post-translational	904:921	arg1	modifications					923:935	Post-translational modifications	904:935	Post-translational modifications	904:935	Post-translational modifications and ultrastructure of collagens were not significantly different; however, LC-MS/MS analysis showed more protein was secreted by Hspg2 cartilage in vitro, suggesting that the incorporation of newly synthesized ECM was impaired.					
32381549	3	37	theme	mass	678:681	arg1	spectrometry					683:694	liquid chromatography-tandem mass spectrometry	649:694	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	649:705	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	37	theme	mass	678:681	arg1	LC-MS/MS					697:704	LC-MS/MS	697:704	LC-MS/MS	697:704	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	6	38	from	osteoarthritis	1413:1426	arg1	SJS					1431:1433	SJS	1431:1433	SJS	1431:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	1	39	theme	critical	134:141	arg1	Perlecan					120:127	Perlecan	120:127	Perlecan	120:127	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	1	39	theme	critical	134:141	arg1	proteoglycan					143:154	a critical proteoglycan	132:154	a critical proteoglycan found in the extracellular matrix (ECM) of cartilage	132:207	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	6	40	theme	functional	1360:1369	arg1	assembly					1381:1388	functional cartilage assembly	1360:1388	functional cartilage assembly	1360:1388	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	6	41	theme	perlecan	1348:1355	arg1	influence					1335:1343	the influence	1331:1343	the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS	1331:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	1	42	theme	extracellular	169:181	arg1	ECM					191:193	ECM	191:193	ECM	191:193	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	1	42	theme	extracellular	169:181	arg1	matrix					183:188	the extracellular matrix	165:188	the extracellular matrix (ECM) of cartilage	165:207	Perlecan is a critical proteoglycan found in the extracellular matrix (ECM) of cartilage.					
32381549	6	43	from	influence	1335:1343	arg1	assembly					1381:1388	functional cartilage assembly	1360:1388	functional cartilage assembly	1360:1388	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	6	43	from	influence	1335:1343	arg1	progression					1398:1408	the progression	1394:1408	the progression of osteoarthritis in SJS	1394:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	5	44	from	decrease	1263:1270	arg1	mechanics					1275:1283	mechanics	1275:1283	mechanics	1275:1283	In addition, glycosaminoglycan deposition was atypical, which may explain the previously observed decrease in mechanics.					
32381549	2	45	theme	healthy	213:219	arg1	cartilage					221:229	healthy cartilage	213:229	healthy cartilage	213:229	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	3	46	theme	liquid	649:654	arg1	spectrometry					683:694	liquid chromatography-tandem mass spectrometry	649:694	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	649:705	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	46	theme	liquid	649:654	arg1	LC-MS/MS					697:704	LC-MS/MS	697:704	LC-MS/MS	697:704	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	2	47	theme	ECM	355:357	arg1	stiffness					359:367	ECM stiffness	355:367	ECM stiffness	355:367	In healthy cartilage, perlecan regulates cartilage biomechanics and we previously demonstrated perlecan deficiency leads to reduced cellular and ECM stiffness in vivo This change in mechanics may lead to the early onset osteoarthritis seen in disorders resulting from perlecan knockdown such as Schwartz-Jampel syndrome (SJS).					
32381549	3	48	theme	ECM	853:855	arg1	collagens					882:890	many collagens	877:890	many collagens	877:890	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	48	theme	ECM	853:855	arg1	proteins					857:864	bulk ECM proteins	848:864	bulk ECM proteins	848:864	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	49	theme	proteins	857:864	arg1	abundance					835:843	the abundance	831:843	the abundance of bulk ECM proteins, including many collagens,	831:891	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	3	50	theme	matrix	805:810	arg1	formation					812:820	defective pericellular matrix formation	782:820	defective pericellular matrix formation	782:820	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	6	51	from	SJS	1431:1433	arg1	assembly					1381:1388	functional cartilage assembly	1360:1388	functional cartilage assembly	1360:1388	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	6	51	from	SJS	1431:1433	arg1	progression					1398:1408	the progression	1394:1408	the progression of osteoarthritis in SJS	1394:1433	Overall, these findings provide insight into the influence of perlecan on functional cartilage assembly and the progression of osteoarthritis in SJS.					
32381549	3	52	used	used	632:635	arg2	we					629:630	we	629:630	we	629:630	To identify how perlecan knockdown affects the material properties of developing cartilage, we used imaging and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study the ECM in a murine model of SJS, Hspg2 Perlecan knockdown led to defective pericellular matrix formation, whereas the abundance of bulk ECM proteins, including many collagens, increased.					
32381549	5	53	theme	glycosaminoglycan	1178:1194	arg1	deposition					1196:1205	glycosaminoglycan deposition	1178:1205	glycosaminoglycan deposition	1178:1205	In addition, glycosaminoglycan deposition was atypical, which may explain the previously observed decrease in mechanics.					
34773866	13	0	theme	broiler	2399:2405	arg1	chickens					2407:2414	broiler chickens	2399:2414	broiler chickens	2399:2414	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	9	1	theme	following	1479:1487	arg1	re-feeding					1489:1498	following re-feeding	1479:1498	following re-feeding for 2 h (P < 0.001)	1479:1518	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	6	2	theme	1,4	1079:1081	arg1	-amylase					1083:1090	α-(1,4)-amylase	1076:1090	α-(1,4)-amylase at 14 d and 42 d (P < 0.05)	1076:1118	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	11	3	theme	relative	1796:1803	arg1	abundance					1805:1813	The relative abundance	1792:1813	The relative abundance of Unclassified_Bacillaceae	1792:1841	The relative abundance of Unclassified_Bacillaceae was moderately positively correlated (r > 0.50) while Oscillospira, Ornithobacterium and Unclassified_Barnesiellaceae were moderately negatively correlated (-0.50 < r -0.39) with starch digestibility coefficient.					
34773866	6	4	theme	α-	1076:1077	arg1	-amylase					1083:1090	α-(1,4)-amylase	1076:1090	α-(1,4)-amylase at 14 d and 42 d (P < 0.05)	1076:1118	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	3	5	from	chickens	587:594	arg1	utilisation					526:536	the utilisation	522:536	the utilisation of corn starch and glucose metabolism in broiler chickens	522:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	3	6	theme	exogenous	477:485	arg1	supplementation					503:517	exogenous α-(1,4)-amylase supplementation	477:517	exogenous α-(1,4)-amylase supplementation	477:517	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	1	7	theme	important	134:142	arg1	Starch					115:120	Starch	115:120	Starch	115:120	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	1	7	theme	important	134:142	arg1	source					151:156	the most important energy source	125:156	the most important energy source in corn-based diets for broiler chickens	125:197	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	10	8	theme	digestibility	1753:1765	arg1	r = -0.35					1780:1788	r = -0.35	1780:1788	r = -0.35	1780:1788	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	10	8	theme	digestibility	1753:1765	arg1	coefficient					1767:1777	starch digestibility coefficient	1746:1777	starch digestibility coefficient (r = -0.35)	1746:1789	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	13	9	theme	starch	2389:2394	arg1	utilisation					2374:2384	the utilisation	2370:2384	the utilisation of starch in broiler chickens	2370:2414	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	3	10	theme	supplementation	503:517	arg1	effects					466:472	the effects	462:472	the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens	462:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	5	11	dep	coefficients	874:885	arg1	nutrient					851:858	The growth performance, nutrient digestibility coefficients	827:885	nutrient	851:858	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	0	12	theme	metabolism	83:92	arg1	utilisation					44:54	the utilisation	40:54	the utilisation of corn starch and glucose metabolism in broiler chickens	40:112	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	5	13	theme	cecal	953:957	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	13	theme	cecal	953:957	arg1	composition					970:980	cecal microbiota composition	953:980	cecal microbiota composition	953:980	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	10	14	theme	starch	1586:1591	arg1	coefficient					1607:1617	starch digestibility coefficient	1586:1617	starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91)	1586:1661	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	1	15	theme	corn-based	161:170	arg1	diets					172:176	corn-based diets	161:176	corn-based diets	161:176	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	13	16	from	utilisation	2374:2384	arg1	chickens					2407:2414	broiler chickens	2399:2414	broiler chickens	2399:2414	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	0	17	from	utilisation	44:54	arg1	chickens					105:112	broiler chickens	97:112	broiler chickens	97:112	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	14	18	theme	chickens	2660:2667	arg1	performance					2637:2647	the growth performance	2626:2647	the growth performance of broiler chickens	2626:2667	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	0	19	theme	broiler	97:103	arg1	chickens					105:112	broiler chickens	97:112	broiler chickens	97:112	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	8	20	theme	galactose	1302:1310	arg1	expression					1331:1340	galactose transporter 1 mRNA expression	1302:1340	galactose transporter 1 mRNA expression	1302:1340	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	13	21	theme	1,4	2334:2336	arg1	-amylase					2338:2345	exogenous α-(1,4)-amylase	2321:2345	exogenous α-(1,4)-amylase	2321:2345	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	5	22	theme	glucose	915:921	arg1	transporter					923:933	glucose transporter	915:933	glucose transporter transcription	915:947	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	4	23	theme	α-	791:792	arg1	-amylase					798:805	0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase	759:805	0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase	759:805	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	14	24	theme	growth	2630:2635	arg1	performance					2637:2647	the growth performance	2626:2647	the growth performance of broiler chickens	2626:2667	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	4	25	theme	broiler	644:650	arg1	chicks					652:657	male broiler chicks	639:657	male broiler chicks	639:657	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	4	25	theme	broiler	644:650	arg1	560					608:610	560	608:610	560	608:610	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	6	26	from	d	1098:1098	arg1	-amylase					1083:1090	α-(1,4)-amylase	1076:1090	α-(1,4)-amylase at 14 d and 42 d (P < 0.05)	1076:1118	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	13	27	theme	-amylase	2338:2345	arg1	addition					2309:2316	excessive addition	2299:2316	excessive addition of exogenous α-(1,4)-amylase	2299:2345	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	3	28	theme	glucose	557:563	arg1	metabolism					565:574	corn starch and glucose metabolism	541:574	metabolism	565:574	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	14	29	from	digestibility	2551:2563	arg1	intestine					2578:2586	the small intestine	2568:2586	the small intestine to a varying degree	2568:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	3	30	theme	starch	546:551	arg1	utilisation					526:536	the utilisation	522:536	the utilisation of corn starch and glucose metabolism in broiler chickens	522:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	14	31	theme	U/kg	2442:2445	arg1	-amylase					2454:2461	Supplement more than 600 U/kg α-(1,4)-amylase	2417:2461	Supplement more than 600 U/kg α-(1,4)-amylase	2417:2461	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	4	32	theme	Arbour	621:626	arg1	560					608:610	560	608:610	560	608:610	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	4	32	theme	Arbour	621:626	arg1	Acres					628:632	4-d-old Arbour Acres	613:632	4-d-old Arbour Acres	613:632	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	14	33	from	coefficients	2509:2520	arg1	intestine					2578:2586	the small intestine	2568:2586	the small intestine to a varying degree	2568:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	9	34	theme	α-	1373:1374	arg1	-amylase					1380:1387	Exogenous α-(1,4)-amylase	1363:1387	Exogenous α-(1,4)-amylase	1363:1387	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	0	35	from	Effects	0:6	arg1	utilisation					44:54	the utilisation	40:54	the utilisation of corn starch and glucose metabolism in broiler chickens	40:112	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	12	36	theme	metabolic	2101:2109	arg1	function					2111:2118	metabolic function	2101:2118	metabolic function	2101:2118	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	37	theme	exogenous	2071:2079	arg1	α-amylase					2081:2089	exogenous α-amylase	2071:2089	exogenous α-amylase	2071:2089	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	0	38	theme	corn	59:62	arg1	starch					64:69	corn starch	59:69	corn starch	59:69	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	1	39	theme	feed	295:298	arg1	utilisation					300:310	feed utilisation	295:310	feed utilisation	295:310	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	5	40	theme	growth	831:836	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	40	theme	growth	831:836	arg1	activity					905:912	digestive enzyme activity	888:912	digestive enzyme activity	888:912	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	40	theme	growth	831:836	arg1	transcription					935:947	glucose transporter transcription	915:947	glucose transporter transcription	915:947	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	40	theme	growth	831:836	arg1	composition					970:980	cecal microbiota composition	953:980	cecal microbiota composition	953:980	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	14	41	theme	Supplement	2417:2426	arg1	-amylase					2454:2461	Supplement more than 600 U/kg α-(1,4)-amylase	2417:2461	Supplement more than 600 U/kg α-(1,4)-amylase	2417:2461	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	3	42	from	starch	546:551	arg1	chickens					587:594	broiler chickens	579:594	broiler chickens	579:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	4	43	theme	corn-soybean	718:729	arg1	diet					736:739	a corn-soybean meal diet	716:739	a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase	716:805	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	13	44	theme	α-	2331:2332	arg1	-amylase					2338:2345	exogenous α-(1,4)-amylase	2321:2345	exogenous α-(1,4)-amylase	2321:2345	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	2	45	theme	supplementing	324:336	arg1	way					376:378	a feasible way	365:378	a feasible way to improve the utilisation of dietary starch for poultry	365:435	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	2	45	theme	supplementing	324:336	arg1	amylase					348:354	supplementing exogenous amylase	324:354	supplementing exogenous amylase	324:354	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	1	46	theme	dietary	228:234	arg1	starch					236:241	dietary starch	228:241	dietary starch	228:241	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	14	47	theme	digestibility	2551:2563	arg1	coefficients					2509:2520	the coefficients	2505:2520	the coefficients of starch and organic matter digestibility in the small intestine to a varying degree	2505:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	8	48	theme	α-	1235:1236	arg1	-amylase					1242:1249	exogenous α-(1,4)-amylase	1225:1249	exogenous α-(1,4)-amylase	1225:1249	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	5	49	theme	digestive	888:896	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	49	theme	digestive	888:896	arg1	activity					905:912	digestive enzyme activity	888:912	digestive enzyme activity	888:912	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	2	50	theme	feasible	367:374	arg1	way					376:378	a feasible way	365:378	a feasible way to improve the utilisation of dietary starch for poultry	365:435	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	2	50	theme	feasible	367:374	arg1	amylase					348:354	supplementing exogenous amylase	324:354	supplementing exogenous amylase	324:354	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	0	51	theme	exogenous	11:19	arg1	-amylase					28:35	exogenous α-(1,4)-amylase	11:35	exogenous α-(1,4)-amylase	11:35	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	13	52	theme	excessive	2299:2307	arg1	addition					2309:2316	excessive addition	2299:2316	excessive addition of exogenous α-(1,4)-amylase	2299:2345	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	7	53	theme	jejunal	1167:1173	arg1	content					1175:1181	jejunal content	1167:1181	jejunal content	1167:1181	However, the endogenous α-amylase activity in jejunal content was linearly decreased (P = 0.045).					
34773866	2	54	theme	starch	418:423	arg1	utilisation					395:405	the utilisation	391:405	the utilisation of dietary starch for poultry	391:435	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	14	55	theme	small	2572:2576	arg1	intestine					2578:2586	the small intestine	2568:2586	the small intestine to a varying degree	2568:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	8	56	theme	1,4	1238:1240	arg1	-amylase					1242:1249	exogenous α-(1,4)-amylase	1225:1249	exogenous α-(1,4)-amylase	1225:1249	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	14	57	from	intestine	2578:2586	arg1	coefficients					2509:2520	the coefficients	2505:2520	the coefficients of starch and organic matter digestibility in the small intestine to a varying degree	2505:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	9	58	theme	serum	1427:1431	arg1	concentrations					1433:1446	glucagon serum concentrations	1418:1446	glucagon serum concentrations	1418:1446	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	11	59	theme	digestibility	2029:2041	arg1	coefficient					2043:2053	starch digestibility coefficient	2022:2053	starch digestibility coefficient	2022:2053	The relative abundance of Unclassified_Bacillaceae was moderately positively correlated (r > 0.50) while Oscillospira, Ornithobacterium and Unclassified_Barnesiellaceae were moderately negatively correlated (-0.50 < r -0.39) with starch digestibility coefficient.					
34773866	14	60	dep	transporters	2481:2492	arg1	decreases					2495:2503	decreases	2495:2503	decreases	2495:2503	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	7	61	dep	decreased	1196:1204	arg1	P = 0.045					1207:1215	P = 0.045	1207:1215	P = 0.045	1207:1215	However, the endogenous α-amylase activity in jejunal content was linearly decreased (P = 0.045).					
34773866	6	62	theme	digestibility	1019:1031	arg1	coefficient					1033:1043	Starch digestibility coefficient	1012:1043	Starch digestibility coefficient	1012:1043	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	5	63	theme	enzyme	898:903	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	63	theme	enzyme	898:903	arg1	activity					905:912	digestive enzyme activity	888:912	digestive enzyme activity	888:912	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	1	64	theme	broiler	182:188	arg1	chickens					190:197	broiler chickens	182:197	broiler chickens	182:197	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	5	65	theme	digestibility	860:872	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	65	theme	digestibility	860:872	arg1	activity					905:912	digestive enzyme activity	888:912	digestive enzyme activity	888:912	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	65	theme	digestibility	860:872	arg1	transcription					935:947	glucose transporter transcription	915:947	glucose transporter transcription	915:947	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	65	theme	digestibility	860:872	arg1	composition					970:980	cecal microbiota composition	953:980	cecal microbiota composition	953:980	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	14	66	theme	organic	2536:2542	arg1	digestibility					2551:2563	organic matter digestibility	2536:2563	organic matter digestibility	2536:2563	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	8	67	theme	Na+-dependent	1276:1288	arg1	glucose					1290:1296	ileal Na+-dependent glucose	1270:1296	ileal Na+-dependent glucose	1270:1296	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	10	68	from	d	1650:1650	arg1	coefficient					1607:1617	starch digestibility coefficient	1586:1617	starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91)	1586:1661	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	14	69	theme	starch	2525:2530	arg1	coefficients					2509:2520	the coefficients	2505:2520	the coefficients of starch and organic matter digestibility in the small intestine to a varying degree	2505:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	10	70	theme	feed	1533:1536	arg1	intake					1538:1543	feed intake	1533:1543	feed intake	1533:1543	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	3	71	from	metabolism	565:574	arg1	chickens					587:594	broiler chickens	579:594	broiler chickens	579:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	10	72	theme	relative	1677:1684	arg1	weight					1686:1691	the relative weight	1673:1691	the relative weight of the gizzard	1673:1706	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	3	73	theme	α-	487:488	arg1	supplementation					503:517	exogenous α-(1,4)-amylase supplementation	477:517	exogenous α-(1,4)-amylase supplementation	477:517	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	3	74	from	effects	466:472	arg1	utilisation					526:536	the utilisation	522:536	the utilisation of corn starch and glucose metabolism in broiler chickens	522:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	10	75	theme	gizzard	1700:1706	arg1	weight					1686:1691	the relative weight	1673:1691	the relative weight of the gizzard	1673:1706	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	3	76	theme	-amylase	494:501	arg1	supplementation					503:517	exogenous α-(1,4)-amylase supplementation	477:517	exogenous α-(1,4)-amylase supplementation	477:517	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	1	77	theme	energy	144:149	arg1	Starch					115:120	Starch	115:120	Starch	115:120	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	1	77	theme	energy	144:149	arg1	source					151:156	the most important energy source	125:156	the most important energy source in corn-based diets for broiler chickens	125:197	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	6	78	theme	quadratic	1054:1062	arg1	response					1064:1071	a quadratic response	1052:1071	a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05)	1052:1118	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	10	79	theme	starch	1746:1751	arg1	r = -0.35					1780:1788	r = -0.35	1780:1788	r = -0.35	1780:1788	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	10	79	theme	starch	1746:1751	arg1	coefficient					1767:1777	starch digestibility coefficient	1746:1777	starch digestibility coefficient (r = -0.35)	1746:1789	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	3	80	theme	1,4	490:492	arg1	supplementation					503:517	exogenous α-(1,4)-amylase supplementation	477:517	exogenous α-(1,4)-amylase supplementation	477:517	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	9	81	dep	higher	1399:1404	arg1	concentrations					1433:1446	glucagon serum concentrations	1418:1446	glucagon serum concentrations	1418:1446	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	9	81	dep	higher	1399:1404	arg1	insulin					1406:1412	insulin	1406:1412	insulin	1406:1412	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	7	82	theme	endogenous	1134:1143	arg1	activity					1155:1162	the endogenous α-amylase activity	1130:1162	the endogenous α-amylase activity in jejunal content	1130:1181	However, the endogenous α-amylase activity in jejunal content was linearly decreased (P = 0.045).					
34773866	5	83	theme	microbiota	959:968	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	83	theme	microbiota	959:968	arg1	composition					970:980	cecal microbiota composition	953:980	cecal microbiota composition	953:980	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	14	84	theme	glucose	2473:2479	arg1	transporters					2481:2492	glucose transporters	2473:2492	glucose transporters	2473:2492	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	8	85	theme	transporter	1312:1322	arg1	expression					1331:1340	galactose transporter 1 mRNA expression	1302:1340	galactose transporter 1 mRNA expression	1302:1340	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	14	86	theme	broiler	2652:2658	arg1	chickens					2660:2667	broiler chickens	2652:2667	broiler chickens	2652:2667	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	11	87	dep	correlated	1988:1997	arg1	-0.50 < r -0.39					2000:2014	-0.50 < r -0.39	2000:2014	-0.50 < r -0.39	2000:2014	The relative abundance of Unclassified_Bacillaceae was moderately positively correlated (r > 0.50) while Oscillospira, Ornithobacterium and Unclassified_Barnesiellaceae were moderately negatively correlated (-0.50 < r -0.39) with starch digestibility coefficient.					
34773866	4	88	dep	-amylase	798:805	arg1	1,4					794:796	1,4	794:796	1,4	794:796	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	12	89	dep	jejunum	2242:2248	arg1	the					2238:2240	the	2238:2240	the	2238:2240	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	90	from	impact	2207:2212	arg1	ileum					2254:2258	ileum	2254:2258	ileum	2254:2258	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	90	from	impact	2207:2212	arg1	jejunum					2242:2248	jejunum	2242:2248	jejunum	2242:2248	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	91	theme	influenced	2171:2180	arg1	microbiota					2186:2195	influenced gut microbiota	2171:2195	influenced gut microbiota which may impact starch digestibility in the jejunum and ileum	2171:2258	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	91	theme	influenced	2171:2180	arg1	concentrations					2155:2168	insulin and glucagon concentrations	2134:2168	insulin and glucagon concentrations	2134:2168	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	8	92	theme	mRNA	1326:1329	arg1	expression					1331:1340	galactose transporter 1 mRNA expression	1302:1340	galactose transporter 1 mRNA expression	1302:1340	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	5	93	theme	transporter	923:933	arg1	transcription					935:947	glucose transporter transcription	915:947	glucose transporter transcription	915:947	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	93	theme	transporter	923:933	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	1	94	from	decrease	283:290	arg1	utilisation					300:310	feed utilisation	295:310	feed utilisation	295:310	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	10	95	theme	digestibility	1593:1605	arg1	coefficient					1607:1617	starch digestibility coefficient	1586:1617	starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91)	1586:1661	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	4	96	theme	U/kg	786:789	arg1	-amylase					798:805	0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase	759:805	0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase	759:805	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	4	97	theme	male	639:642	arg1	chicks					652:657	male broiler chicks	639:657	male broiler chicks	639:657	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	4	97	theme	male	639:642	arg1	560					608:610	560	608:610	560	608:610	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	6	98	from	d	1107:1107	arg1	-amylase					1083:1090	α-(1,4)-amylase	1076:1090	α-(1,4)-amylase at 14 d and 42 d (P < 0.05)	1076:1118	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	14	99	theme	matter	2544:2549	arg1	digestibility					2551:2563	organic matter digestibility	2536:2563	organic matter digestibility	2536:2563	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	14	100	from	starch	2525:2530	arg1	intestine					2578:2586	the small intestine	2568:2586	the small intestine to a varying degree	2568:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	3	101	theme	corn	541:544	arg1	starch					546:551	corn starch and glucose metabolism	541:574	starch	546:551	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	1	102	from	source	151:156	arg1	diets					172:176	corn-based diets	161:176	corn-based diets	161:176	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	14	103	theme	α-	2447:2448	arg1	-amylase					2454:2461	Supplement more than 600 U/kg α-(1,4)-amylase	2417:2461	Supplement more than 600 U/kg α-(1,4)-amylase	2417:2461	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	4	104	theme	560	608:610	arg1	total					599:603	A total	597:603	A total of 560, 4-d-old Arbour Acres plus male broiler chicks	597:657	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	9	105	theme	Exogenous	1363:1371	arg1	-amylase					1380:1387	Exogenous α-(1,4)-amylase	1363:1387	Exogenous α-(1,4)-amylase	1363:1387	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	12	106	theme	gut	2182:2184	arg1	microbiota					2186:2195	influenced gut microbiota	2171:2195	influenced gut microbiota which may impact starch digestibility in the jejunum and ileum	2171:2258	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	106	theme	gut	2182:2184	arg1	concentrations					2155:2168	insulin and glucagon concentrations	2134:2168	insulin and glucagon concentrations	2134:2168	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	4	107	theme	4-d-old	613:619	arg1	560					608:610	560	608:610	560	608:610	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	4	107	theme	4-d-old	613:619	arg1	Acres					628:632	4-d-old Arbour Acres	613:632	4-d-old Arbour Acres	613:632	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	0	108	theme	starch	64:69	arg1	utilisation					44:54	the utilisation	40:54	the utilisation of corn starch and glucose metabolism in broiler chickens	40:112	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	11	109	theme	Unclassified_Bacillaceae	1818:1841	arg1	abundance					1805:1813	The relative abundance	1792:1813	The relative abundance of Unclassified_Bacillaceae	1792:1841	The relative abundance of Unclassified_Bacillaceae was moderately positively correlated (r > 0.50) while Oscillospira, Ornithobacterium and Unclassified_Barnesiellaceae were moderately negatively correlated (-0.50 < r -0.39) with starch digestibility coefficient.					
34773866	12	110	theme	insulin	2134:2140	arg1	microbiota					2186:2195	influenced gut microbiota	2171:2195	influenced gut microbiota which may impact starch digestibility in the jejunum and ileum	2171:2258	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	110	theme	insulin	2134:2140	arg1	concentrations					2155:2168	insulin and glucagon concentrations	2134:2168	insulin and glucagon concentrations	2134:2168	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	9	111	theme	1,4	1376:1378	arg1	-amylase					1380:1387	Exogenous α-(1,4)-amylase	1363:1387	Exogenous α-(1,4)-amylase	1363:1387	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	0	112	theme	glucose	75:81	arg1	metabolism					83:92	glucose metabolism	75:92	glucose metabolism	75:92	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	5	113	theme	performance	838:848	arg1	coefficients					874:885	The growth performance, nutrient digestibility coefficients	827:885	coefficients	874:885	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	113	theme	performance	838:848	arg1	activity					905:912	digestive enzyme activity	888:912	digestive enzyme activity	888:912	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	113	theme	performance	838:848	arg1	transcription					935:947	glucose transporter transcription	915:947	glucose transporter transcription	915:947	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	5	113	theme	performance	838:848	arg1	composition					970:980	cecal microbiota composition	953:980	cecal microbiota composition	953:980	The growth performance, nutrient digestibility coefficients, digestive enzyme activity, glucose transporter transcription and cecal microbiota composition were evaluated in this study.					
34773866	12	114	theme	glucagon	2146:2153	arg1	microbiota					2186:2195	influenced gut microbiota	2171:2195	influenced gut microbiota which may impact starch digestibility in the jejunum and ileum	2171:2258	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	12	114	theme	glucagon	2146:2153	arg1	concentrations					2155:2168	insulin and glucagon concentrations	2134:2168	insulin and glucagon concentrations	2134:2168	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	4	115	theme	treatment	691:699	arg1	groups					701:706	five treatment groups	686:706	five treatment groups	686:706	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	2	116	theme	exogenous	338:346	arg1	way					376:378	a feasible way	365:378	a feasible way to improve the utilisation of dietary starch for poultry	365:435	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	2	116	theme	exogenous	338:346	arg1	amylase					348:354	supplementing exogenous amylase	324:354	supplementing exogenous amylase	324:354	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	13	117	theme	study	2281:2285	arg1	results					2265:2271	The results	2261:2271	The results of this study	2261:2285	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	10	118	from	d	1630:1630	arg1	coefficient					1607:1617	starch digestibility coefficient	1586:1617	starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91)	1586:1661	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	14	119	theme	varying	2593:2599	arg1	degree					2601:2606	a varying degree	2591:2606	a varying degree	2591:2606	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	11	120	theme	starch	2022:2027	arg1	coefficient					2043:2053	starch digestibility coefficient	2022:2053	starch digestibility coefficient	2022:2053	The relative abundance of Unclassified_Bacillaceae was moderately positively correlated (r > 0.50) while Oscillospira, Ornithobacterium and Unclassified_Barnesiellaceae were moderately negatively correlated (-0.50 < r -0.39) with starch digestibility coefficient.					
34773866	14	121	dep	-amylase	2454:2461	arg1	1,4					2450:2452	1,4	2450:2452	1,4	2450:2452	Supplement more than 600 U/kg α-(1,4)-amylase inhibited glucose transporters, decreases the coefficients of starch and organic matter digestibility in the small intestine to a varying degree, thereby limiting the growth performance of broiler chickens.					
34773866	12	122	theme	starch	2214:2219	arg1	digestibility					2221:2233	starch digestibility	2214:2233	starch digestibility	2214:2233	In conclusion, exogenous α-amylase modulates metabolic function by increasing insulin and glucagon concentrations, influenced gut microbiota which may impact starch digestibility in the jejunum and ileum.					
34773866	0	123	theme	α-	21:22	arg1	-amylase					28:35	exogenous α-(1,4)-amylase	11:35	exogenous α-(1,4)-amylase	11:35	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	1	124	theme	starch	236:241	arg1	proportion					214:223	some proportion	209:223	some proportion of dietary starch	209:241	Starch is the most important energy source in corn-based diets for broiler chickens, however, some proportion of dietary starch may be partially digested, leading to a decrease in feed utilisation.					
34773866	13	125	theme	exogenous	2321:2329	arg1	-amylase					2338:2345	exogenous α-(1,4)-amylase	2321:2345	exogenous α-(1,4)-amylase	2321:2345	The results of this study showed that excessive addition of exogenous α-(1,4)-amylase was not beneficial for the utilisation of starch in broiler chickens.					
34773866	3	126	theme	broiler	579:585	arg1	chickens					587:594	broiler chickens	579:594	broiler chickens	579:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	8	127	theme	exogenous	1225:1233	arg1	-amylase					1242:1249	exogenous α-(1,4)-amylase	1225:1249	exogenous α-(1,4)-amylase	1225:1249	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
34773866	3	128	from	utilisation	526:536	arg1	chickens					587:594	broiler chickens	579:594	broiler chickens	579:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	0	129	theme	1,4	24:26	arg1	-amylase					28:35	exogenous α-(1,4)-amylase	11:35	exogenous α-(1,4)-amylase	11:35	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	9	130	theme	glucagon	1418:1425	arg1	concentrations					1433:1446	glucagon serum concentrations	1418:1446	glucagon serum concentrations	1418:1446	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	7	131	theme	α-amylase	1145:1153	arg1	activity					1155:1162	the endogenous α-amylase activity	1130:1162	the endogenous α-amylase activity in jejunal content	1130:1181	However, the endogenous α-amylase activity in jejunal content was linearly decreased (P = 0.045).					
34773866	2	132	theme	dietary	410:416	arg1	starch					418:423	dietary starch	410:423	dietary starch	410:423	Therefore, supplementing exogenous amylase might be a feasible way to improve the utilisation of dietary starch for poultry.					
34773866	3	133	theme	metabolism	565:574	arg1	utilisation					526:536	the utilisation	522:536	the utilisation of corn starch and glucose metabolism in broiler chickens	522:594	This study investigated the effects of exogenous α-(1,4)-amylase supplementation on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	4	134	theme	meal	731:734	arg1	diet					736:739	a corn-soybean meal diet	716:739	a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase	716:805	A total of 560, 4-d-old Arbour Acres plus male broiler chicks were randomly divided into five treatment groups and fed a corn-soybean meal diet supplemented with 0, 400, 600, 800 and 1 200 U/kg α-(1,4)-amylase to evaluate growth.					
34773866	0	135	theme	-amylase	28:35	arg1	Effects					0:6	Effects	0:6	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.	0:113	Effects of exogenous α-(1,4)-amylase on the utilisation of corn starch and glucose metabolism in broiler chickens.					
34773866	7	136	from	activity	1155:1162	arg1	content					1175:1181	jejunal content	1167:1181	jejunal content	1167:1181	However, the endogenous α-amylase activity in jejunal content was linearly decreased (P = 0.045).					
34773866	10	137	theme	BW	1521:1522	arg1	gain					1524:1527	BW gain	1521:1527	BW gain	1521:1527	BW gain and feed intake were strongly positively correlated with starch digestibility coefficient at both 14 d (r = 0.87) and 42 d (r = 0.91); whereas, the relative weight of the gizzard was weakly negatively correlated with starch digestibility coefficient (r = -0.35).					
34773866	9	138	theme	fasted	1451:1456	arg1	birds					1458:1462	fasted birds	1451:1462	fasted birds	1451:1462	Exogenous α-(1,4)-amylase generated higher insulin and glucagon serum concentrations in fasted birds (P < 0.05) and following re-feeding for 2 h (P < 0.001).					
34773866	6	139	theme	Starch	1012:1017	arg1	coefficient					1033:1043	Starch digestibility coefficient	1012:1043	Starch digestibility coefficient	1012:1043	Starch digestibility coefficient showed a quadratic response to α-(1,4)-amylase at 14 d and 42 d (P < 0.05).					
34773866	11	140	dep	correlated	1869:1878	arg1	r > 0.50					1881:1888	r > 0.50	1881:1888	r > 0.50	1881:1888	The relative abundance of Unclassified_Bacillaceae was moderately positively correlated (r > 0.50) while Oscillospira, Ornithobacterium and Unclassified_Barnesiellaceae were moderately negatively correlated (-0.50 < r -0.39) with starch digestibility coefficient.					
34773866	8	141	theme	ileal	1270:1274	arg1	glucose					1290:1296	ileal Na+-dependent glucose	1270:1296	ileal Na+-dependent glucose	1270:1296	Also, exogenous α-(1,4)-amylase linearly decreased ileal Na+-dependent glucose and galactose transporter 1 mRNA expression at 42 d (P < 0.001).					
33739906	0	0	theme	ocular	94:99	arg1	inflammation					101:112	ocular inflammation	94:112	ocular inflammation	94:112	Nanodelivery of triamcinolone acetonide with PLGA-chitosan nanoparticles for the treatment of ocular inflammation.					
33739906	3	1	theme	tumour	661:666	arg1	factor					677:682	tumour necrosis factor	661:682	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	3	1	theme	tumour	661:666	arg1	TNF					685:687	TNF	685:687	TNF	685:687	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	3	2	theme	-α	689:690	arg1	cells					702:706	tumour necrosis factor (TNF)-α activated cells	661:706	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	3	3	from	IL	652:653	arg1	cells					702:706	tumour necrosis factor (TNF)-α activated cells	661:706	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	6	4	theme	Substantial	1195:1205	arg1	concentrations					1207:1220	6 h. Substantial concentrations	1190:1220	6 h. Substantial concentrations of TA	1190:1226	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	6	5	theme	TA-loaded	1162:1170	arg1	NPs					1176:1178	TA-loaded PLC NPs	1162:1178	TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA	1162:1226	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	1	6	theme	clinical	250:257	arg1	benefits					259:266	its clinical benefits	246:266	its clinical benefits	246:266	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	5	7	theme	PLC	928:930	arg1	NPs					932:934	TA-loaded PLC NPs	918:934	TA-loaded PLC NPs	918:934	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	6	8	theme	PLC	1172:1174	arg1	NPs					1176:1178	TA-loaded PLC NPs	1162:1178	TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA	1162:1226	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	6	9	theme	Pharmacokinetic	1108:1122	arg1	analysis					1124:1131	Pharmacokinetic analysis	1108:1131	Pharmacokinetic analysis of rabbit eyes	1108:1146	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	7	10	theme	PLC-based	1328:1336	arg1	formulations					1341:1352	PLC-based NP formulations	1328:1352	PLC-based NP formulations	1328:1352	Overall, PLC-based NP formulations offer a new approach for the treatment of ocular inflammatory diseases.					
33739906	5	11	theme	chamber	1089:1095	arg1	fogginess					1097:1105	anterior chamber fogginess	1080:1105	anterior chamber fogginess	1080:1105	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	2	12	theme	diseases	476:483	arg1	treatment					443:451	the treatment	439:451	the treatment of ocular inflammatory diseases	439:483	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	4	13	theme	inflammation	793:804	arg1	signs					780:784	any typical clinical signs	759:784	any typical clinical signs of eye inflammation	759:804	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	7	14	theme	NP	1338:1339	arg1	formulations					1341:1352	PLC-based NP formulations	1328:1352	PLC-based NP formulations	1328:1352	Overall, PLC-based NP formulations offer a new approach for the treatment of ocular inflammatory diseases.					
33739906	7	15	theme	ocular	1396:1401	arg1	diseases					1416:1423	ocular inflammatory diseases	1396:1423	ocular inflammatory diseases	1396:1423	Overall, PLC-based NP formulations offer a new approach for the treatment of ocular inflammatory diseases.					
33739906	2	16	theme	inflammatory	463:474	arg1	diseases					476:483	ocular inflammatory diseases	456:483	ocular inflammatory diseases	456:483	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	7	17	theme	new	1362:1364	arg1	approach					1366:1373	a new approach	1360:1373	a new approach for the treatment of ocular inflammatory diseases	1360:1423	Overall, PLC-based NP formulations offer a new approach for the treatment of ocular inflammatory diseases.					
33739906	4	18	theme	typical	763:769	arg1	signs					780:784	any typical clinical signs	759:784	any typical clinical signs of eye inflammation	759:804	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	2	19	dep	poly	362:365	arg1	l-lactide-co-glycolide					369:390	l-lactide-co-glycolide	369:390	l-lactide-co-glycolide	369:390	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	2	19	dep	poly	362:365	arg1	d					367:367	d	367:367	d	367:367	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	4	20	theme	eye	789:791	arg1	inflammation					793:804	eye inflammation	789:804	eye inflammation	789:804	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	2	21	theme	ocular	456:461	arg1	diseases					476:483	ocular inflammatory diseases	456:483	ocular inflammatory diseases	456:483	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	3	22	theme	necrosis	668:675	arg1	factor					677:682	tumour necrosis factor	661:682	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	3	22	theme	necrosis	668:675	arg1	TNF					685:687	TNF	685:687	TNF	685:687	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	5	23	from	reduction	1067:1075	arg1	fogginess					1097:1105	anterior chamber fogginess	1080:1105	anterior chamber fogginess	1080:1105	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	3	24	theme	PLC	496:498	arg1	NPs					500:502	TA-loaded PLC NPs	486:502	TA-loaded PLC NPs	486:502	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	4	25	theme	free	869:872	arg1	suspension					877:886	free TA suspension	869:886	free TA suspension	869:886	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	3	26	theme	factor	677:682	arg1	cells					702:706	tumour necrosis factor (TNF)-α activated cells	661:706	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	6	27	theme	eyes	1143:1146	arg1	analysis					1124:1131	Pharmacokinetic analysis	1108:1131	Pharmacokinetic analysis of rabbit eyes	1108:1146	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	3	28	theme	activated	692:700	arg1	cells					702:706	tumour necrosis factor (TNF)-α activated cells	661:706	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	5	29	theme	TA	1041:1042	arg1	suspension					1044:1053	free TA suspension	1036:1053	free TA suspension	1036:1053	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	1	30	theme	several	184:190	arg1	disorders					199:207	several ocular disorders	184:207	several ocular disorders	184:207	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	2	31	theme	PLC	410:412	arg1	NPs					430:432	NPs	430:432	NPs	430:432	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	2	31	theme	PLC	410:412	arg1	nanoparticles					415:427	poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles	362:427	poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs)	362:433	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	1	32	theme	commercial	272:281	arg1	compositions					283:294	commercial compositions	272:294	commercial compositions	272:294	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	3	33	from	cells	702:706	arg1	secretion					626:634	the secretion	622:634	the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells	622:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	7	34	theme	inflammatory	1403:1414	arg1	diseases					1416:1423	ocular inflammatory diseases	1396:1423	ocular inflammatory diseases	1396:1423	Overall, PLC-based NP formulations offer a new approach for the treatment of ocular inflammatory diseases.					
33739906	1	35	theme	ocular	192:197	arg1	disorders					199:207	several ocular disorders	184:207	several ocular disorders	184:207	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	0	36	theme	acetonide	30:38	arg1	Nanodelivery					0:11	Nanodelivery	0:11	Nanodelivery of triamcinolone acetonide with PLGA-chitosan	0:57	Nanodelivery of triamcinolone acetonide with PLGA-chitosan nanoparticles for the treatment of ocular inflammation.					
33739906	4	37	theme	PLC	738:740	arg1	NPs					742:744	TA-loaded PLC NPs	728:744	TA-loaded PLC NPs	728:744	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	5	38	theme	TA-loaded	918:926	arg1	NPs					932:934	TA-loaded PLC NPs	918:934	TA-loaded PLC NPs	918:934	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	5	39	theme	greater	948:954	arg1	humour					964:969	a greater aqueous humour	946:969	a greater aqueous humour transparency (%AHT)	946:989	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	1	40	theme	Triamcinolone	115:127	arg1	TA					140:141	TA	140:141	TA	140:141	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	1	40	theme	Triamcinolone	115:127	arg1	acetonide					129:137	Triamcinolone acetonide	115:137	Triamcinolone acetonide (TA)	115:142	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	1	41	theme	disorders	199:207	arg1	treatment					171:179	the treatment	167:179	the treatment of several ocular disorders	167:207	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	2	42	theme	-chitosan	399:407	arg1	NPs					430:432	NPs	430:432	NPs	430:432	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	2	42	theme	-chitosan	399:407	arg1	nanoparticles					415:427	poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles	362:427	poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs)	362:433	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	3	43	theme	TA-loaded	486:494	arg1	NPs					500:502	TA-loaded PLC NPs	486:502	TA-loaded PLC NPs	486:502	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	5	44	theme	%	985:985	arg1	transparency					971:982	a greater aqueous humour transparency	946:982	a greater aqueous humour transparency (%AHT)	946:989	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	5	44	theme	%	985:985	arg1	AHT					986:988	%AHT	985:988	%AHT	985:988	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	3	45	theme	anti-inflammatory	524:540	arg1	activity					542:549	excellent anti-inflammatory activity	514:549	excellent anti-inflammatory activity against human corneal epithelial (HCE) cells	514:594	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	1	46	theme	adverse	302:308	arg1	effects					317:323	adverse ocular effects	302:323	adverse ocular effects	302:323	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	4	47	theme	TA	874:875	arg1	suspension					877:886	free TA suspension	869:886	free TA suspension	869:886	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	3	48	theme	epithelial	573:582	arg1	cells					590:594	human corneal epithelial (HCE) cells	559:594	human corneal epithelial (HCE) cells	559:594	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	7	49	theme	diseases	1416:1423	arg1	treatment					1383:1391	the treatment	1379:1391	the treatment of ocular inflammatory diseases	1379:1423	Overall, PLC-based NP formulations offer a new approach for the treatment of ocular inflammatory diseases.					
33739906	6	50	theme	system	1311:1316	arg1	superiority					1269:1279	the superiority	1265:1279	the superiority of this PLC-based nanocarrier system	1265:1316	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	1	51	theme	ocular	310:315	arg1	effects					317:323	adverse ocular effects	302:323	adverse ocular effects	302:323	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	5	52	theme	anterior	1080:1087	arg1	fogginess					1097:1105	anterior chamber fogginess	1080:1105	anterior chamber fogginess	1080:1105	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	6	53	theme	TA	1225:1226	arg1	concentrations					1207:1220	6 h. Substantial concentrations	1190:1220	6 h. Substantial concentrations of TA	1190:1226	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	4	54	dep	typical	763:769	arg1	clinical					771:778	clinical	771:778	clinical	771:778	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	3	55	from	secretion	626:634	arg1	cells					702:706	tumour necrosis factor (TNF)-α activated cells	661:706	tumour necrosis factor (TNF)-α activated cells	661:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	6	56	theme	nanocarrier	1299:1309	arg1	system					1311:1316	this PLC-based nanocarrier system	1284:1316	this PLC-based nanocarrier system	1284:1316	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	5	57	theme	humour	964:969	arg1	transparency					971:982	a greater aqueous humour transparency	946:982	a greater aqueous humour transparency (%AHT)	946:989	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	5	57	theme	humour	964:969	arg1	AHT					986:988	%AHT	985:988	%AHT	985:988	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	3	58	theme	human	559:563	arg1	cells					590:594	human corneal epithelial (HCE) cells	559:594	human corneal epithelial (HCE) cells	559:594	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	6	59	theme	rabbit	1136:1141	arg1	eyes					1143:1146	rabbit eyes	1136:1146	rabbit eyes	1136:1146	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	6	60	theme	PLC-based	1289:1297	arg1	system					1311:1316	this PLC-based nanocarrier system	1284:1316	this PLC-based nanocarrier system	1284:1316	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	5	61	theme	free	1036:1039	arg1	suspension					1044:1053	free TA suspension	1036:1053	free TA suspension	1036:1053	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	5	62	theme	normal	1014:1019	arg1	saline					1021:1026	normal saline	1014:1026	normal saline (NS)	1014:1031	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	5	62	theme	normal	1014:1019	arg1	NS					1029:1030	NS	1029:1030	NS	1029:1030	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	5	63	theme	aqueous	956:962	arg1	humour					964:969	a greater aqueous humour	946:969	a greater aqueous humour transparency (%AHT)	946:989	TA-loaded PLC NPs exhibited a greater aqueous humour transparency (%AHT), compared with that of normal saline (NS) or free TA suspension, indicating reduction in anterior chamber fogginess.					
33739906	3	64	theme	corneal	565:571	arg1	cells					590:594	human corneal epithelial (HCE) cells	559:594	human corneal epithelial (HCE) cells	559:594	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	4	65	theme	TA-loaded	728:736	arg1	NPs					742:744	TA-loaded PLC NPs	728:744	TA-loaded PLC NPs	728:744	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	6	66	theme	6 h.	1190:1193	arg1	concentrations					1207:1220	6 h. Substantial concentrations	1190:1220	6 h. Substantial concentrations of TA	1190:1226	Pharmacokinetic analysis of rabbit eyes revealed that TA-loaded PLC NPs peaked at 6 h. Substantial concentrations of TA were observed until 24 h, indicating the superiority of this PLC-based nanocarrier system.					
33739906	3	67	theme	IL	652:653	arg1	secretion					626:634	the secretion	622:634	the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells	622:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	0	68	with	Nanodelivery	0:11	arg1	PLGA-chitosan					45:57	PLGA-chitosan	45:57	PLGA-chitosan	45:57	Nanodelivery of triamcinolone acetonide with PLGA-chitosan nanoparticles for the treatment of ocular inflammation.					
33739906	3	69	theme	excellent	514:522	arg1	activity					542:549	excellent anti-inflammatory activity	514:549	excellent anti-inflammatory activity against human corneal epithelial (HCE) cells	514:594	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	1	70	theme	free	218:221	arg1	suspension					228:237	the free drug suspension	214:237	the free drug suspension	214:237	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	4	71	theme	inflammatory	835:846	arg1	signs					848:852	inflammatory signs	835:852	inflammatory signs	835:852	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	4	72	theme	rabbit	714:719	arg1	model					721:725	a rabbit model	712:725	a rabbit model	712:725	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	1	73	theme	drug	223:226	arg1	suspension					228:237	the free drug suspension	214:237	the free drug suspension	214:237	Triamcinolone acetonide (TA) is widely indicated in the treatment of several ocular disorders, but the free drug suspension limits its clinical benefits and commercial compositions cause adverse ocular effects.					
33739906	3	74	theme	interleukin	639:649	arg1	IL					652:653	interleukin (IL)-6	639:656	interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells	639:706	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	3	75	theme	HCE	585:587	arg1	cells					590:594	human corneal epithelial (HCE) cells	559:594	human corneal epithelial (HCE) cells	559:594	TA-loaded PLC NPs exhibited excellent anti-inflammatory activity against human corneal epithelial (HCE) cells and significantly reduced the secretion of interleukin (IL)-6 in tumour necrosis factor (TNF)-α activated cells.					
33739906	2	76	theme	poly	362:365	arg1	NPs					430:432	NPs	430:432	NPs	430:432	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	2	76	theme	poly	362:365	arg1	nanoparticles					415:427	poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles	362:427	poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs)	362:433	In this study, TA was formulated in poly(d,l-lactide-co-glycolide) (PLGA)-chitosan (PLC) nanoparticles (NPs) for the treatment of ocular inflammatory diseases.					
33739906	4	77	theme	single	905:910	arg1	dose					912:915	a single dose	903:915	a single dose	903:915	In a rabbit model, TA-loaded PLC NPs did not show any typical clinical signs of eye inflammation and significantly alleviated inflammatory signs, compared with free TA suspension, at 24 h after a single dose.					
33739906	0	78	theme	triamcinolone	16:28	arg1	acetonide					30:38	triamcinolone acetonide	16:38	triamcinolone acetonide	16:38	Nanodelivery of triamcinolone acetonide with PLGA-chitosan nanoparticles for the treatment of ocular inflammation.					
33739906	0	79	theme	inflammation	101:112	arg1	treatment					81:89	the treatment	77:89	the treatment of ocular inflammation	77:112	Nanodelivery of triamcinolone acetonide with PLGA-chitosan nanoparticles for the treatment of ocular inflammation.					
31999034	2	0	from	species 	191:198	arg1	profile					122:128	The profile	118:128	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	118:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	6	1	with	chromatography	1210:1223	arg1	spectrometry					1235:1246	mass spectrometry	1230:1246	mass spectrometry	1230:1246	However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry.					
31999034	7	2	theme	origin	1358:1363	arg1	composition					1324:1334	the flavanols and proanthocyanidins composition	1288:1334	the flavanols and proanthocyanidins composition of rose hip and their origin	1288:1363	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	2	3	theme	cellulose	336:344	arg1	plates					346:351	high performance thin layer chromatography cellulose plates	293:351	high performance thin layer chromatography cellulose plates	293:351	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	4	dep	species 	191:198	arg1	spinosissima					262:273	spinosissima	262:273	spinosissima	262:273	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	4	dep	species 	191:198	arg1	canina					203:208	canina	203:208	canina	203:208	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	4	dep	species 	191:198	arg1	glutinosa					214:222	R. glutinosa	211:222	R. glutinosa	211:222	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	4	dep	species 	191:198	arg1	multiflora					243:252	multiflora	243:252	multiflora	243:252	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	5	theme	proanthocyanidins	150:166	arg1	profile					122:128	The profile	118:128	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	118:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	3	6	theme	anthocyanins	553:564	arg1	indication					539:548	indication	539:548	indication of anthocyanins	539:564	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	7	7	theme	liquid	1430:1435	arg1	spectrometry					1457:1468	liquid chromatography/mass spectrometry	1430:1468	liquid chromatography/mass spectrometry data	1430:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	2	8	theme	flavan-3-ols	133:144	arg1	profile					122:128	The profile	118:128	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	118:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	4	9	dep	dimers	834:839	arg1	hexosides					853:861	hexosides	853:861	hexosides	853:861	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	4	10	gly	glycosylated	665:676	arg1	flavan-3-ols					678:689	glycosylated flavan-3-ols	665:689	glycosylated flavan-3-ols	665:689	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	2	11	from	profile	122:128	arg1	species 					191:198	five different Rosa species 	171:198	five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	171:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	12	from	flavan-3-ols	133:144	arg1	species 					191:198	five different Rosa species 	171:198	five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	171:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	0	13	from	hip	94:96	arg1	fingerprints					33:44	the chromatographic fingerprints	13:44	the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip	13:96	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	6	14	theme	proanthocyanidin	1140:1155	arg1	trihexoside					1164:1174	proanthocyanidin trimer trihexoside	1140:1174	proanthocyanidin trimer trihexoside	1140:1174	However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry.					
31999034	4	15	theme	glycosylated	665:676	arg1	flavan-3-ols					678:689	glycosylated flavan-3-ols	665:689	glycosylated flavan-3-ols	665:689	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	5	16	theme	flavanols	878:886	arg1	total					866:870	A total	864:870	A total of 15 flavanols and their derivatives	864:908	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	2	17	from	proanthocyanidins	150:166	arg1	species 					191:198	five different Rosa species 	171:198	five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	171:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	4	18	theme	catechin	755:762	arg1	dimers					834:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	2	19	theme	R.	211:212	arg1	canina					203:208	canina	203:208	canina	203:208	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	2	19	theme	R.	211:212	arg1	glutinosa					214:222	R. glutinosa	211:222	R. glutinosa	211:222	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	5	20	theme	trap	989:992	arg1	analyzer					1019:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	4	21	theme	thin	604:607	arg1	chromatography					615:628	thin layer chromatography	604:628	thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	604:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	9	22	theme	hips	1666:1669	arg1	profiles					1639:1646	flavanol and proanthocyanidin profiles	1609:1646	flavanol and proanthocyanidin profiles of different rose hips	1609:1669	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	7	23	theme	hip	1344:1346	arg1	composition					1324:1334	the flavanols and proanthocyanidins composition	1288:1334	the flavanols and proanthocyanidins composition of rose hip and their origin	1288:1363	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	5	24	theme	liquid	955:960	arg1	chromatography					962:975	ultra-high-performance liquid chromatography	932:975	ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer	932:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	4	25	theme	proanthocyanidin	789:804	arg1	dimer					806:810	proanthocyanidin dimer	789:810	proanthocyanidin dimer	789:810	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	0	26	theme	Rosa	99:102	arg1	sp					104:105	Rosa sp	99:105	Rosa sp	99:105	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	9	27	theme	geographical	1685:1696	arg1	origin					1698:1703	the geographical origin	1681:1703	the geographical origin	1681:1703	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	9	28	theme	different	1651:1659	arg1	hips					1666:1669	different rose hips	1651:1669	different rose hips	1651:1669	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	7	29	theme	proanthocyanidins	1306:1322	arg1	composition					1324:1334	the flavanols and proanthocyanidins composition	1288:1334	the flavanols and proanthocyanidins composition of rose hip and their origin	1288:1363	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	7	30	theme	data	1470:1473	arg1	set					1423:1425	the entire set	1412:1425	the entire set of liquid chromatography/mass spectrometry data	1412:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	7	30	theme	data	1470:1473	arg1	data					1470:1473	liquid chromatography/mass spectrometry data	1430:1473	liquid chromatography/mass spectrometry data	1430:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	5	31	with	chromatography	962:975	arg1	analyzer					1019:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	8	32	theme	scores	1503:1508	arg1	plots					1510:1514	Both principal components' scores plots	1476:1514	Both principal components' scores plots	1476:1514	Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier.					
31999034	7	33	theme	flavanols	1292:1300	arg1	composition					1324:1334	the flavanols and proanthocyanidins composition	1288:1334	the flavanols and proanthocyanidins composition of rose hip and their origin	1288:1363	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	5	34	theme	mass	1014:1017	arg1	analyzer					1019:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	7	35	theme	chromatography/mass	1437:1455	arg1	spectrometry					1457:1468	liquid chromatography/mass spectrometry	1430:1468	liquid chromatography/mass spectrometry data	1430:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	4	36	theme	hexoside	779:786	arg1	dimers					834:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	0	37	theme	chromatographic	17:31	arg1	fingerprints					33:44	the chromatographic fingerprints	13:44	the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip	13:96	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	2	38	theme	different Rosa	176:189	arg1	species 					191:198	five different Rosa species 	171:198	five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima)	171:274	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	8	39	theme	Rosa	1528:1531	arg1	spinosissima					1533:1544	Rosa spinosissima	1528:1544	Rosa spinosissima	1528:1544	Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier.					
31999034	8	39	theme	Rosa	1528:1531	arg1	outlier					1572:1578	an outlier	1569:1578	an outlier	1569:1578	Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier.					
31999034	7	40	theme	entire	1416:1421	arg1	set					1423:1425	the entire set	1412:1425	the entire set of liquid chromatography/mass spectrometry data	1412:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	7	40	theme	entire	1416:1421	arg1	data					1470:1473	liquid chromatography/mass spectrometry data	1430:1473	liquid chromatography/mass spectrometry data	1430:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	2	41	theme	chromatography	321:334	arg1	plates					346:351	high performance thin layer chromatography cellulose plates	293:351	high performance thin layer chromatography cellulose plates	293:351	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	6	42	theme	mass	1230:1233	arg1	spectrometry					1235:1246	mass spectrometry	1230:1246	mass spectrometry	1230:1246	However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry.					
31999034	4	43	theme	proanthocyanidin	817:832	arg1	dimers					834:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	2	44	theme	thin	310:313	arg1	chromatography					321:334	high performance thin layer chromatography	293:334	high performance thin layer chromatography cellulose plates	293:351	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	5	45	theme	proanthocyanidin	1064:1079	arg1	trimer					1081:1086	proanthocyanidin trimer	1064:1086	proanthocyanidin trimer	1064:1086	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	4	46	theme	layer	609:613	arg1	chromatography					615:628	thin layer chromatography	604:628	thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	604:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	3	47	theme	red	526:528	arg1	zones					530:534	the only detected flavanol and red zones	495:534	zones	530:534	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	0	48	dep	Establishing	0:11	arg1	sp					104:105	Rosa sp	99:105	Rosa sp	99:105	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	7	49	theme	component	1376:1384	arg1	analysis					1386:1393	principal component analysis	1366:1393	principal component analysis	1366:1393	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	4	50	theme	elution	584:590	arg1	solvent					592:598	the elution solvent	580:598	the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	580:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	4	51	theme	flavan-3-ols	678:689	arg1	analyses					653:660	mass spectrometry analyses	635:660	mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	635:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	2	52	theme	high	293:296	arg1	chromatography					321:334	high performance thin layer chromatography	293:334	high performance thin layer chromatography cellulose plates	293:351	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	5	53	theme	derivatives	898:908	arg1	total					866:870	A total	864:870	A total of 15 flavanols and their derivatives	864:908	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	4	54	theme	proanthocyanidins	695:711	arg1	analyses					653:660	mass spectrometry analyses	635:660	mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	635:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	4	55	theme	spectrometry	640:651	arg1	analyses					653:660	mass spectrometry analyses	635:660	mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	635:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	2	56	theme	layer	315:319	arg1	chromatography					321:334	high performance thin layer chromatography	293:334	high performance thin layer chromatography cellulose plates	293:351	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	9	57	theme	flavanol	1609:1616	arg1	profiles					1639:1646	flavanol and proanthocyanidin profiles	1609:1646	flavanol and proanthocyanidin profiles of different rose hips	1609:1669	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	3	58	from	Differences	354:364	arg1	profiles					399:406	flavanol and proanthocyanidin profiles	369:406	profiles	399:406	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	4	59	with	chromatography	615:628	arg1	analyses					653:660	mass spectrometry analyses	635:660	mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	635:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	9	60	theme	proanthocyanidin	1622:1637	arg1	profiles					1639:1646	flavanol and proanthocyanidin profiles	1609:1646	flavanol and proanthocyanidin profiles of different rose hips	1609:1669	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	5	61	theme	linear	982:987	arg1	analyzer					1019:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	0	62	theme	rose	89:92	arg1	hip					94:96	rose hip	89:96	rose hip	89:96	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	9	63	theme	rose	1661:1664	arg1	hips					1666:1669	different rose hips	1651:1669	different rose hips	1651:1669	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	9	64	dep	cultivar	1724:1731	arg1	the					1720:1722	the	1720:1722	the	1720:1722	Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.					
31999034	7	65	theme	rose	1339:1342	arg1	hip					1344:1346	rose hip	1339:1346	rose hip	1339:1346	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	5	66	theme	ultra-high-performance	932:953	arg1	chromatography					962:975	ultra-high-performance liquid chromatography	932:975	ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer	932:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	7	67	theme	spectrometry	1457:1468	arg1	data					1470:1473	liquid chromatography/mass spectrometry data	1430:1473	liquid chromatography/mass spectrometry data	1430:1473	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	4	68	theme	dimer	806:810	arg1	dimers					834:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers	755:839	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	5	69	theme	quadrupole-Orbitrap	994:1012	arg1	analyzer					1019:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	linear trap quadrupole-Orbitrap mass analyzer	982:1026	A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique.					
31999034	6	70	theme	trimer	1157:1162	arg1	trihexoside					1164:1174	proanthocyanidin trimer trihexoside	1140:1174	proanthocyanidin trimer trihexoside	1140:1174	However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry.					
31999034	3	71	theme	proanthocyanidin	382:397	arg1	profiles					399:406	flavanol and proanthocyanidin profiles	369:406	profiles	399:406	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	4	72	theme	epi	766:768	arg1	hexoside					779:786	(epi)catechin hexoside	765:786	(epi)catechin hexoside	765:786	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	3	73	theme	flavanol	369:376	arg1	profiles					399:406	flavanol and proanthocyanidin profiles	369:406	profiles	399:406	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	4	74	theme	catechin	770:777	arg1	hexoside					779:786	(epi)catechin hexoside	765:786	(epi)catechin hexoside	765:786	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	6	75	theme	thin-layer	1199:1208	arg1	chromatography					1210:1223	thin-layer chromatography	1199:1223	thin-layer chromatography with mass spectrometry	1199:1246	However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry.					
31999034	0	76	theme	flavan-3-ols	49:60	arg1	fingerprints					33:44	the chromatographic fingerprints	13:44	the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip	13:96	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	3	77	theme	extracts	415:422	arg1	profiles					399:406	flavanol and proanthocyanidin profiles	369:406	profiles	399:406	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	4	78	theme	trimers	845:851	arg1	identification					737:750	identification	737:750	identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides	737:861	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	0	79	theme	proanthocyanidins	66:82	arg1	fingerprints					33:44	the chromatographic fingerprints	13:44	the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip	13:96	Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.)					
31999034	3	80	theme	detected	504:511	arg1	flavanol					513:520	the only detected flavanol and red zones	495:534	flavanol	513:520	Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins.					
31999034	8	81	theme	principal	1481:1489	arg1	plots					1510:1514	Both principal components' scores plots	1476:1514	Both principal components' scores plots	1476:1514	Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier.					
31999034	2	82	theme	performance	298:308	arg1	chromatography					321:334	high performance thin layer chromatography	293:334	high performance thin layer chromatography cellulose plates	293:351	The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates.					
31999034	7	83	theme	principal	1366:1374	arg1	analysis					1386:1393	principal component analysis	1366:1393	principal component analysis	1366:1393	To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data.					
31999034	4	84	theme	mass	635:638	arg1	analyses					653:660	mass spectrometry analyses	635:660	mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins	635:711	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
31999034	4	85	theme	dimers	834:839	arg1	identification					737:750	identification	737:750	identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides	737:861	Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides.					
32027899	8	0	theme	reused	939:944	arg1	times					949:953	reused 20 times	939:953	reused 20 times	939:953	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	0	1	theme	dyes	101:104	arg1	degradation					78:88	biocatalytic degradation	65:88	biocatalytic degradation of organic dyes	65:104	Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes.					
32027899	9	2	theme	decolorization	1117:1130	arg1	activity					1132:1139	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity	1064:1139	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase	1064:1162	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	9	3	theme	long-term	1194:1202	arg1	activity					1231:1238	long-term textile dye decolorization activity	1194:1238	long-term textile dye decolorization activity	1194:1238	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	6	4	theme	optimum	659:665	arg1	temperature					667:677	the optimum temperature	655:677	the optimum temperature	655:677	It was observed that immobilized laccase achieved maximum activity at pH 4.0 and the optimum temperature was found to be 50 °C.					
32027899	6	4	theme	optimum	659:665	arg1	50 °C					695:699	50 °C	695:699	50 °C	695:699	It was observed that immobilized laccase achieved maximum activity at pH 4.0 and the optimum temperature was found to be 50 °C.					
32027899	7	5	theme	residual	753:760	arg1	activity					829:836	its initial activity	817:836	its initial activity	817:836	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	5	theme	residual	753:760	arg1	%					804:804	87%	802:804	87%	802:804	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	5	theme	residual	753:760	arg1	activity					762:769	the residual activity	749:769	the residual activity of the immobilized laccase	749:796	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	6	from	+4 °C	719:723	arg1	storage					708:714	storage	708:714	storage at +4 °C and ~25 °C for 4 weeks	708:746	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	8	7	theme	relative	960:967	arg1	activity					1044:1051	its initial activity	1032:1051	its initial activity	1032:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	7	theme	relative	960:967	arg1	%					1027:1027	approximately 50%	1011:1027	approximately 50% of its initial activity	1011:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	7	theme	relative	960:967	arg1	activity					969:976	the relative activity	956:976	the relative activity of immobilized laccase	956:999	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	9	8	theme	dye	1076:1078	arg1	activity					1132:1139	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity	1064:1139	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase	1064:1162	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	7	9	from	~25 °C	729:734	arg1	storage					708:714	storage	708:714	storage at +4 °C and ~25 °C for 4 weeks	708:746	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	9	10	theme	immobilized	1144:1154	arg1	laccase					1156:1162	immobilized laccase	1144:1162	immobilized laccase	1144:1162	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	8	11	from	%	916:916	arg1	180 min					921:927	180 min	921:927	180 min	921:927	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	6	12	theme	immobilized	595:605	arg1	laccase					607:613	immobilized laccase	595:613	immobilized laccase	595:613	It was observed that immobilized laccase achieved maximum activity at pH 4.0 and the optimum temperature was found to be 50 °C.					
32027899	9	13	theme	laccase	1156:1162	arg1	activity					1132:1139	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity	1064:1139	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase	1064:1162	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	5	14	theme	immobilized	519:529	arg1	laccase					531:537	the immobilized laccase	515:537	the immobilized laccase	515:537	The biochemical properties of the immobilized laccase were comprehensively investigated.					
32027899	11	15	theme	magnetic	1372:1379	arg1	composite					1388:1396	this functionalized magnetic hybrid composite	1352:1396	this functionalized magnetic hybrid composite	1352:1396	Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme.					
32027899	3	16	theme	several	398:404	arg1	methods					406:412	several methods	398:412	several methods	398:412	The support was characterized using several methods.					
32027899	8	17	theme	initial	1036:1042	arg1	activity					1044:1051	its initial activity	1032:1051	its initial activity	1032:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	2	18	theme	thiolated	336:344	arg1	TCS					356:358	TCS	356:358	TCS	356:358	Firstly, Fe3O4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS).					
32027899	2	18	theme	thiolated	336:344	arg1	chitosan					346:353	thiolated chitosan	336:353	thiolated chitosan (TCS)	336:359	Firstly, Fe3O4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS).					
32027899	5	19	theme	laccase	531:537	arg1	properties					501:510	The biochemical properties	485:510	The biochemical properties of the immobilized laccase	485:537	The biochemical properties of the immobilized laccase were comprehensively investigated.					
32027899	1	20	theme	enzyme	190:195	arg1	stability					197:205	enzyme stability	190:205	enzyme stability	190:205	In this study, a novel immobilization support for laccase was developed to enhance enzyme stability, efficiency and reusability.					
32027899	8	21	theme	laccase	993:999	arg1	activity					1044:1051	its initial activity	1032:1051	its initial activity	1032:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	21	theme	laccase	993:999	arg1	%					1027:1027	approximately 50%	1011:1027	approximately 50% of its initial activity	1011:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	21	theme	laccase	993:999	arg1	activity					969:976	the relative activity	956:976	the relative activity of immobilized laccase	956:999	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	7	22	theme	activity	829:836	arg1	activity					829:836	its initial activity	817:836	its initial activity	817:836	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	22	theme	activity	829:836	arg1	%					804:804	87%	802:804	87%	802:804	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	22	theme	activity	829:836	arg1	%					812:812	80%	810:812	80% of its initial activity	810:836	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	22	theme	activity	829:836	arg1	activity					762:769	the residual activity	749:769	the residual activity of the immobilized laccase	749:796	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	0	23	theme	hybrid	44:49	arg1	composite					51:59	chitosan-Fe3O4 hybrid composite	29:59	chitosan-Fe3O4 hybrid composite	29:59	Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes.					
32027899	8	24	theme	immobilized	981:991	arg1	laccase					993:999	immobilized laccase	981:999	immobilized laccase	981:999	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	25	theme	immobilized	879:889	arg1	laccase					891:897	immobilized laccase	879:897	immobilized laccase	879:897	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	0	26	theme	chitosan-Fe3O4	29:42	arg1	composite					51:59	chitosan-Fe3O4 hybrid composite	29:59	chitosan-Fe3O4 hybrid composite	29:59	Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes.					
32027899	8	27	theme	activity	1044:1051	arg1	activity					1044:1051	its initial activity	1032:1051	its initial activity	1032:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	27	theme	activity	1044:1051	arg1	activity					969:976	the relative activity	956:976	the relative activity of immobilized laccase	956:999	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	8	27	theme	activity	1044:1051	arg1	%					1027:1027	approximately 50%	1011:1027	approximately 50% of its initial activity	1011:1051	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	2	28	theme	co-precipitation	307:322	arg1	route					324:328	the co-precipitation route	303:328	the co-precipitation route	303:328	Firstly, Fe3O4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS).					
32027899	5	29	theme	biochemical	489:499	arg1	properties					501:510	The biochemical properties	485:510	The biochemical properties of the immobilized laccase	485:537	The biochemical properties of the immobilized laccase were comprehensively investigated.					
32027899	4	30	attach	attached	438:445	arg2	laccase					426:432	laccase	426:432	laccase	426:432	Afterward, laccase was attached to the surface of functional support.					
32027899	4	30	attach	attached	438:445	arg2	Afterward					415:423	Afterward	415:423	Afterward	415:423	Afterward, laccase was attached to the surface of functional support.					
32027899	4	30	attach	attached	438:445	arg1	surface					454:460	the surface	450:460	the surface of functional support	450:482	Afterward, laccase was attached to the surface of functional support.					
32027899	6	31	theme	maximum	624:630	arg1	activity					632:639	maximum activity	624:639	maximum activity	624:639	It was observed that immobilized laccase achieved maximum activity at pH 4.0 and the optimum temperature was found to be 50 °C.					
32027899	2	32	theme	Fe3O4	245:249	arg1	particles					260:268	Fe3O4 magnetic particles	245:268	Fe3O4 magnetic particles	245:268	Firstly, Fe3O4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS).					
32027899	7	33	theme	initial	821:827	arg1	activity					829:836	its initial activity	817:836	its initial activity	817:836	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	4	34	theme	support	476:482	arg1	surface					454:460	the surface	450:460	the surface of functional support	450:482	Afterward, laccase was attached to the surface of functional support.					
32027899	9	35	theme	Reactive	1081:1088	arg1	171					1095:1097	Reactive Blue 171	1081:1097	Reactive Blue 171	1081:1097	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	11	36	theme	hybrid	1381:1386	arg1	composite					1388:1396	this functionalized magnetic hybrid composite	1352:1396	this functionalized magnetic hybrid composite	1352:1396	Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme.					
32027899	4	37	theme	functional	465:474	arg1	support					476:482	functional support	465:482	functional support	465:482	Afterward, laccase was attached to the surface of functional support.					
32027899	9	38	theme	Blue	1090:1093	arg1	171					1095:1097	Reactive Blue 171	1081:1097	Reactive Blue 171	1081:1097	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	0	39	theme	biocatalytic	65:76	arg1	degradation					78:88	biocatalytic degradation	65:88	biocatalytic degradation of organic dyes	65:104	Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes.					
32027899	7	40	theme	laccase	790:796	arg1	activity					829:836	its initial activity	817:836	its initial activity	817:836	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	40	theme	laccase	790:796	arg1	%					804:804	87%	802:804	87%	802:804	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	7	40	theme	laccase	790:796	arg1	activity					762:769	the residual activity	749:769	the residual activity of the immobilized laccase	749:796	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	9	41	theme	textile	1204:1210	arg1	activity					1231:1238	long-term textile dye decolorization activity	1194:1238	long-term textile dye decolorization activity	1194:1238	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	8	42	theme	laccase	891:897	arg1	activity					867:874	the activity	863:874	the activity of immobilized laccase	863:897	At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity.					
32027899	10	43	theme	laccase	1284:1290	arg1	use					1277:1279	the use	1273:1279	the use of laccase in industrial and biotechnological applications	1273:1338	These results are promising for the use of laccase in industrial and biotechnological applications.					
32027899	1	44	theme	novel	124:128	arg1	support					145:151	a novel immobilization support	122:151	a novel immobilization support for laccase	122:163	In this study, a novel immobilization support for laccase was developed to enhance enzyme stability, efficiency and reusability.					
32027899	11	45	used	used	1407:1410	arg2	composite					1388:1396	this functionalized magnetic hybrid composite	1352:1396	this functionalized magnetic hybrid composite	1352:1396	Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme.					
32027899	9	46	theme	dye	1212:1214	arg1	activity					1231:1238	long-term textile dye decolorization activity	1194:1238	long-term textile dye decolorization activity	1194:1238	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	1	47	theme	immobilization	130:143	arg1	support					145:151	a novel immobilization support	122:151	a novel immobilization support for laccase	122:163	In this study, a novel immobilization support for laccase was developed to enhance enzyme stability, efficiency and reusability.					
32027899	9	48	theme	decolorization	1216:1229	arg1	activity					1231:1238	long-term textile dye decolorization activity	1194:1238	long-term textile dye decolorization activity	1194:1238	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	10	49	theme	industrial	1295:1304	arg1	applications					1327:1338	industrial and biotechnological applications	1295:1338	applications	1327:1338	These results are promising for the use of laccase in industrial and biotechnological applications.					
32027899	9	50	dep	dye	1076:1078	arg1	171					1095:1097	Reactive Blue 171	1081:1097	Reactive Blue 171	1081:1097	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	9	50	dep	dye	1076:1078	arg1	Acid					1103:1106	Acid Blue 74	1103:1114	Acid Blue 74	1103:1114	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	9	51	theme	textile	1068:1074	arg1	dye					1076:1078	textile dye	1068:1078	the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase	1064:1162	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	11	52	theme	important	1451:1459	arg1	enzyme					1461:1466	an industrially important enzyme	1435:1466	an industrially important enzyme	1435:1466	Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme.					
32027899	11	52	theme	important	1451:1459	arg1	laccase					1426:1432	laccase	1426:1432	laccase	1426:1432	Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme.					
32027899	7	53	theme	immobilized	778:788	arg1	laccase					790:796	the immobilized laccase	774:796	the immobilized laccase	774:796	After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively.					
32027899	0	54	theme	organic	93:99	arg1	dyes					101:104	organic dyes	93:104	organic dyes	93:104	Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes.					
32027899	10	55	from	use	1277:1279	arg1	applications					1327:1338	industrial and biotechnological applications	1295:1338	applications	1327:1338	These results are promising for the use of laccase in industrial and biotechnological applications.					
32027899	9	56	theme	Blue	1108:1111	arg1	Acid					1103:1106	Acid Blue 74	1103:1114	Acid Blue 74	1103:1114	Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity.					
32027899	11	57	theme	functionalized	1357:1370	arg1	composite					1388:1396	this functionalized magnetic hybrid composite	1352:1396	this functionalized magnetic hybrid composite	1352:1396	Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme.					
32027899	2	58	theme	magnetic	251:258	arg1	particles					260:268	Fe3O4 magnetic particles	245:268	Fe3O4 magnetic particles	245:268	Firstly, Fe3O4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS).					
32027899	10	59	theme	biotechnological	1310:1325	arg1	applications					1327:1338	industrial and biotechnological applications	1295:1338	applications	1327:1338	These results are promising for the use of laccase in industrial and biotechnological applications.					
32178417	6	0	from	°C	970:971	arg1	kg/m2∙h					956:962	18.61 × 10-2 kg/m2∙h	943:962	18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed	943:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	0	from	°C	970:971	arg1	J					937:937	J	937:937	J	937:937	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	0	from	°C	970:971	arg1	flux					932:935	a flux	930:935	a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed	930:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	1	1	theme	acid-co-maleic	254:267	arg1	acid					269:272	styrene sulfonic acid-co-maleic acid	237:272	styrene sulfonic acid-co-maleic acid	237:272	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	1	1	theme	acid-co-maleic	254:267	arg1	poly					232:235	poly	232:235	poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA)	232:286	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	7	2	theme	water	1043:1047	arg1	flux					1022:1025	The total flux	1012:1025	The total flux	1012:1025	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	7	2	theme	water	1043:1047	arg1	flux					1035:1038	the flux	1031:1038	the flux of water	1031:1047	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	6	3	theme	mass	845:848	arg1	%					849:849	40 mass%	842:849	40 mass% of titanium dioxide	842:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	3	theme	mass	845:848	arg1	dioxide					863:869	titanium dioxide	854:869	titanium dioxide	854:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	10	4	theme	activation	1579:1588	arg1	energies					1590:1597	The estimated activation energies	1565:1597	The estimated activation energies for total permeation (EP) and total diffusion (ED) values	1565:1655	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	6	5	from	flux	932:935	arg1	°C					970:971	30 °C	967:971	30 °C for 10 mass% w/w of water in the feed	967:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	7	6	used	used	1139:1142	arg2	membranes					1110:1118	these membranes	1104:1118	these membranes	1104:1118	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	5	7	theme	pervaporation	662:674	arg1	cell					676:679	a pervaporation cell	660:679	a pervaporation cell fitted with each membrane in order to evaluate the extent of the pervaporation dehydration of isopropanol	660:785	Subsequently, we used a pervaporation cell fitted with each membrane in order to evaluate the extent of the pervaporation dehydration of isopropanol.					
32178417	9	8	theme	separation	1512:1521	arg1	ability					1523:1529	a high separation ability	1505:1529	a high separation ability for the water-isopropanol system	1505:1562	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	0	9	theme	Isopropanol	103:113	arg1	Dehydration					88:98	the Dehydration	84:98	the Dehydration of Isopropanol by Pervaporation	84:130	Crosslinked Nanocomposite Sodium Alginate-Based Membranes with Titanium Dioxide for the Dehydration of Isopropanol by Pervaporation.					
32178417	6	10	theme	×	949:949	arg1	kg/m2∙h					956:962	18.61 × 10-2 kg/m2∙h	943:962	18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed	943:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	11	theme	kg/m2∙h	956:962	arg1	J					937:937	J	937:937	J	937:937	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	11	theme	kg/m2∙h	956:962	arg1	flux					932:935	a flux	930:935	a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed	930:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	12	theme	24,092	917:922	arg1	α					911:911	α	911:911	α	911:911	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	12	theme	24,092	917:922	arg1	factor					904:909	the highest separation factor	881:909	the highest separation factor(α) of 24,092	881:922	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	7	13	theme	total	1016:1020	arg1	flux					1022:1025	The total flux	1012:1025	The total flux	1012:1025	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	6	14	theme	10-2	951:954	arg1	kg/m2∙h					956:962	18.61 × 10-2 kg/m2∙h	943:962	18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed	943:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	4	15	theme	water	606:610	arg1	compositions					586:597	different compositions	576:597	different compositions of the water and isopropanol mixtures	576:635	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32178417	2	16	theme	dioxide	393:399	arg1	dioxide					393:399	titanium dioxide	384:399	titanium dioxide with respect to sodium alginate	384:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	2	16	theme	dioxide	393:399	arg1	%					375:375	0, 10, 20, 30 and 40% w/w	355:379	0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate	355:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	9	17	theme	water-isopropanol	1539:1555	arg1	system					1557:1562	the water-isopropanol system	1535:1562	the water-isopropanol system	1535:1562	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	10	18	theme	diffusion	1635:1643	arg1	values					1650:1655	total diffusion (ED) values	1629:1655	total diffusion (ED) values	1629:1655	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	1	19	theme	solution	195:202	arg1	technique					204:212	a solution technique	193:212	a solution technique	193:212	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	0	20	theme	Alginate-Based	33:46	arg1	Membranes					48:56	Nanocomposite Sodium Alginate-Based Membranes	12:56	Nanocomposite Sodium Alginate-Based Membranes	12:56	Crosslinked Nanocomposite Sodium Alginate-Based Membranes with Titanium Dioxide for the Dehydration of Isopropanol by Pervaporation.					
32178417	11	21	theme	enthalpy	1785:1792	arg1	values					1794:1799	the enthalpy values	1781:1799	the enthalpy values for all the membranes	1781:1821	The negative change in the enthalpy values for all the membranes indicates that sorption was mainly dominated by Langmuir's mode of sorption.					
32178417	7	22	theme	azeotropic	1157:1166	arg1	point					1168:1172	the azeotropic point	1153:1172	the azeotropic point of water-isopropanol mixtures	1153:1202	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	6	23	theme	water	993:997	arg1	w/w					986:988	10 mass% w/w	977:988	10 mass% w/w of water in the feed	977:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	2	24	theme	w/w	377:379	arg1	dioxide					393:399	titanium dioxide	384:399	titanium dioxide with respect to sodium alginate	384:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	2	24	theme	w/w	377:379	arg1	%					375:375	0, 10, 20, 30 and 40% w/w	355:379	0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate	355:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	5	25	theme	pervaporation	746:758	arg1	dehydration					760:770	the pervaporation dehydration	742:770	the pervaporation dehydration of isopropanol	742:785	Subsequently, we used a pervaporation cell fitted with each membrane in order to evaluate the extent of the pervaporation dehydration of isopropanol.					
32178417	6	26	theme	separation	893:902	arg1	α					911:911	α	911:911	α	911:911	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	26	theme	separation	893:902	arg1	factor					904:909	the highest separation factor	881:909	the highest separation factor(α) of 24,092	881:922	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	10	27	theme	total	1603:1607	arg1	EP					1621:1622	EP	1621:1622	EP	1621:1622	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	10	27	theme	total	1603:1607	arg1	permeation					1609:1618	total permeation	1603:1618	total permeation (EP)	1603:1623	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	8	28	theme	excellent	1280:1288	arg1	performance					1290:1300	an excellent performance	1277:1300	an excellent performance in the dehydration of isopropanol	1277:1334	The results clearly indicate that these nanocomposite membranes exhibit an excellent performance in the dehydration of isopropanol.					
32178417	2	29	theme	%	375:375	arg1	incorporation					338:350	the incorporation	334:350	the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate	334:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	5	30	used	used	655:658	arg2	we					652:653	we	652:653	we	652:653	Subsequently, we used a pervaporation cell fitted with each membrane in order to evaluate the extent of the pervaporation dehydration of isopropanol.					
32178417	6	31	theme	highest	885:891	arg1	α					911:911	α	911:911	α	911:911	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	31	theme	highest	885:891	arg1	factor					904:909	the highest separation factor	881:909	the highest separation factor(α) of 24,092	881:922	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	5	32	theme	isopropanol	775:785	arg1	dehydration					760:770	the pervaporation dehydration	742:770	the pervaporation dehydration of isopropanol	742:785	Subsequently, we used a pervaporation cell fitted with each membrane in order to evaluate the extent of the pervaporation dehydration of isopropanol.					
32178417	10	33	theme	ED	1646:1647	arg1	values					1650:1655	total diffusion (ED) values	1629:1655	total diffusion (ED) values	1629:1655	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	9	34	contain	have	1500:1503	arg2	ability					1523:1529	a high separation ability	1505:1529	a high separation ability for the water-isopropanol system	1505:1562	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	9	34	contain	have	1500:1503	arg1	membranes					1490:1498	these membranes	1484:1498	these membranes	1484:1498	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	4	35	theme	different	576:584	arg1	compositions					586:597	different compositions	576:597	different compositions of the water and isopropanol mixtures	576:635	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32178417	2	36	theme	sodium	417:422	arg1	alginate					424:431	sodium alginate	417:431	sodium alginate	417:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	2	37	theme	titanium	384:391	arg1	dioxide					393:399	titanium dioxide	384:399	titanium dioxide with respect to sodium alginate	384:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	6	38	contain	containing	831:840	arg1	membranes					821:829	the membranes	817:829	the membranes containing 40 mass% of titanium dioxide	817:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	38	contain	containing	831:840	arg2	%					849:849	40 mass%	842:849	40 mass% of titanium dioxide	842:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	38	contain	containing	831:840	arg2	dioxide					863:869	titanium dioxide	854:869	titanium dioxide	854:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	7	39	theme	water-isopropanol	1177:1193	arg1	mixtures					1195:1202	water-isopropanol mixtures	1177:1202	water-isopropanol mixtures	1177:1202	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	10	40	theme	estimated	1569:1577	arg1	energies					1590:1597	The estimated activation energies	1565:1597	The estimated activation energies for total permeation (EP) and total diffusion (ED) values	1565:1655	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	0	41	theme	Titanium	63:70	arg1	Dioxide					72:78	Titanium Dioxide	63:78	Titanium Dioxide for the Dehydration of Isopropanol by Pervaporation	63:130	Crosslinked Nanocomposite Sodium Alginate-Based Membranes with Titanium Dioxide for the Dehydration of Isopropanol by Pervaporation.					
32178417	6	42	theme	dioxide	863:869	arg1	%					849:849	40 mass%	842:849	40 mass% of titanium dioxide	842:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	42	theme	dioxide	863:869	arg1	dioxide					863:869	titanium dioxide	854:869	titanium dioxide	854:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	11	43	theme	sorption	1890:1897	arg1	mode					1882:1885	Langmuir's mode	1871:1885	Langmuir's mode of sorption	1871:1897	The negative change in the enthalpy values for all the membranes indicates that sorption was mainly dominated by Langmuir's mode of sorption.					
32178417	7	44	theme	mixtures	1195:1202	arg1	point					1168:1172	the azeotropic point	1153:1172	the azeotropic point of water-isopropanol mixtures	1153:1202	The total flux and the flux of water were found to overlap with each other, indicating that these membranes can be effectively used to break the azeotropic point of water-isopropanol mixtures.					
32178417	6	45	from	w/w	986:988	arg1	feed					1006:1009	the feed	1002:1009	the feed	1002:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	6	46	theme	%	984:984	arg1	w/w					986:988	10 mass% w/w	977:988	10 mass% w/w of water in the feed	977:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	2	47	mod	modified	322:329	arg1	membranes					307:315	the membranes	303:315	the membranes	303:315	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	2	47	mod	modified	322:329	arg3	incorporation					338:350	the incorporation	334:350	the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate	334:431	Subsequently, the membranes were modified by the incorporation of 0, 10, 20, 30 and 40% w/w of titanium dioxide with respect to sodium alginate.					
32178417	6	48	theme	titanium	854:861	arg1	dioxide					863:869	titanium dioxide	854:869	titanium dioxide	854:869	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	8	49	theme	nanocomposite	1245:1257	arg1	membranes					1259:1267	these nanocomposite membranes	1239:1267	these nanocomposite membranes	1239:1267	The results clearly indicate that these nanocomposite membranes exhibit an excellent performance in the dehydration of isopropanol.					
32178417	4	50	dep	water	606:610	arg1	mixtures					628:635	mixtures	628:635	mixtures	628:635	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32178417	4	50	dep	water	606:610	arg1	the					602:604	the	602:604	the	602:604	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32178417	9	51	theme	isopropanol	1443:1453	arg1	permeation					1455:1464	the isopropanol permeation	1439:1464	the isopropanol permeation	1439:1464	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	6	52	theme	mass	980:983	arg1	w/w					986:988	10 mass% w/w	977:988	10 mass% w/w of water in the feed	977:1009	Among the membranes studied, the membranes containing 40 mass% of titanium dioxide exhibited the highest separation factor(α) of 24,092, with a flux(J) of 18.61 × 10-2 kg/m2∙h at 30 °C for 10 mass% w/w of water in the feed.					
32178417	10	53	theme	total	1629:1633	arg1	values					1650:1655	total diffusion (ED) values	1629:1655	total diffusion (ED) values	1629:1655	The estimated activation energies for total permeation (EP) and total diffusion (ED) values ranged between 10.60 kJ∙mol-1 and 3.96 kJ∙mol-1, and 10.76 kJ∙mol-1 and 4.29 kJ∙mol-1, respectively.					
32178417	4	54	theme	equilibrium	524:534	arg1	experiment					545:554	An equilibrium swelling experiment	521:554	An equilibrium swelling experiment	521:554	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32178417	8	55	theme	isopropanol	1324:1334	arg1	dehydration					1309:1319	the dehydration	1305:1319	the dehydration of isopropanol	1305:1334	The results clearly indicate that these nanocomposite membranes exhibit an excellent performance in the dehydration of isopropanol.					
32178417	9	56	theme	water	1383:1387	arg1	permeation					1389:1398	the water permeation	1379:1398	the water permeation	1379:1398	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	9	57	theme	energy	1352:1357	arg1	lower					1419:1423	lower	1419:1423	lower	1419:1423	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	9	57	theme	energy	1352:1357	arg1	values					1359:1364	The activation energy values	1337:1364	The activation energy values obtained for the water permeation	1337:1398	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	11	58	from	change	1771:1776	arg1	values					1794:1799	the enthalpy values	1781:1799	the enthalpy values for all the membranes	1781:1821	The negative change in the enthalpy values for all the membranes indicates that sorption was mainly dominated by Langmuir's mode of sorption.					
32178417	9	59	theme	activation	1341:1350	arg1	energy					1352:1357	activation energy	1341:1357	The activation energy values obtained for the water permeation	1337:1398	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	9	60	theme	high	1507:1510	arg1	ability					1523:1529	a high separation ability	1505:1529	a high separation ability for the water-isopropanol system	1505:1562	The activation energy values obtained for the water permeation were significantly lower than those of the isopropanol permeation, underlining that these membranes have a high separation ability for the water-isopropanol system.					
32178417	11	61	theme	negative	1762:1769	arg1	change					1771:1776	The negative change	1758:1776	The negative change in the enthalpy values for all the membranes	1758:1821	The negative change in the enthalpy values for all the membranes indicates that sorption was mainly dominated by Langmuir's mode of sorption.					
32178417	5	62	theme	dehydration	760:770	arg1	extent					732:737	the extent	728:737	the extent of the pervaporation dehydration of isopropanol	728:785	Subsequently, we used a pervaporation cell fitted with each membrane in order to evaluate the extent of the pervaporation dehydration of isopropanol.					
32178417	4	63	theme	isopropanol	616:626	arg1	compositions					586:597	different compositions	576:597	different compositions of the water and isopropanol mixtures	576:635	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32178417	1	64	theme	styrene	237:243	arg1	acid					269:272	styrene sulfonic acid-co-maleic acid	237:272	styrene sulfonic acid-co-maleic acid	237:272	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	1	64	theme	styrene	237:243	arg1	poly					232:235	poly	232:235	poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA)	232:286	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	8	65	from	performance	1290:1300	arg1	dehydration					1309:1319	the dehydration	1305:1319	the dehydration of isopropanol	1305:1334	The results clearly indicate that these nanocomposite membranes exhibit an excellent performance in the dehydration of isopropanol.					
32178417	1	66	theme	sulfonic	245:252	arg1	acid					269:272	styrene sulfonic acid-co-maleic acid	237:272	styrene sulfonic acid-co-maleic acid	237:272	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	1	66	theme	sulfonic	245:252	arg1	poly					232:235	poly	232:235	poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA)	232:286	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	1	67	theme	based	157:161	arg1	membranes					163:171	Sodium alginate (NaAlg) based membranes	133:171	Sodium alginate (NaAlg) based membranes	133:171	Sodium alginate (NaAlg) based membranes were prepared using a solution technique, crosslinked with poly(styrene sulfonic acid-co-maleic acid) (PSSA-co-MA).					
32178417	4	68	theme	swelling	536:543	arg1	experiment					545:554	An equilibrium swelling experiment	521:554	An equilibrium swelling experiment	521:554	An equilibrium swelling experiment was performed using different compositions of the water and isopropanol mixtures.					
32446309	6	0	theme	lower	898:902	arg1	pH					912:913	a lower resting pH	896:913	a lower resting pH	896:913	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	6	1	theme	in	865:866	arg1	measurements					876:887	The in vivo pH measurements	861:887	The in vivo pH measurements	861:887	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	2	2	theme	urea	329:332	arg1	concentrations					334:347	electrolyte and urea concentrations	313:347	concentrations	334:347	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	6	3	theme	carious	974:980	arg1	teeth					982:986	carious teeth	974:986	carious teeth	974:986	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	7	4	theme	human	1102:1106	arg1	saliva					1108:1113	human saliva	1102:1113	human saliva	1102:1113	CONCLUSIONS No large differences were found between the composition of equine saliva and human saliva.					
32446309	1	5	theme	infundibular	226:237	arg1	caries					239:244	infundibular caries	226:244	infundibular caries	226:244	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	0	6	theme	oral	75:78	arg1	biofilm					80:86	the oral biofilm	71:86	the oral biofilm of sound and carious tooth surfaces	71:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	7	7	theme	equine	1084:1089	arg1	saliva					1091:1096	equine saliva	1084:1096	equine saliva	1084:1096	CONCLUSIONS No large differences were found between the composition of equine saliva and human saliva.					
32446309	1	8	theme	caries	239:244	arg1	development					211:221	development	211:221	development of infundibular caries in equine cheek teeth	211:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	5	9	theme	human	731:735	arg1	saliva					737:742	stimulated human saliva	720:742	stimulated human saliva	720:742	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	9	10	theme	same	1259:1262	arg1	pattern					1264:1270	the same pattern	1255:1270	the same pattern	1255:1270	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	7	11	theme	saliva	1091:1096	arg1	composition					1069:1079	the composition	1065:1079	the composition of equine saliva and human saliva	1065:1113	CONCLUSIONS No large differences were found between the composition of equine saliva and human saliva.					
32446309	5	12	theme	horse	745:749	arg1	saliva					751:756	horse saliva	745:756	horse saliva	745:756	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	9	13	theme	acid	1301:1304	arg1	production					1306:1315	acid production	1301:1315	acid production by oral microorganisms	1301:1338	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	6	14	theme	sucrose	951:957	arg1	application					959:969	sucrose application	951:969	sucrose application in carious teeth	951:986	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	7	15	dep	CONCLUSIONS	1013:1023	arg1	found					1051:1055	found	1051:1055	were found between the composition of equine saliva and human saliva	1046:1113	CONCLUSIONS No large differences were found between the composition of equine saliva and human saliva.					
32446309	6	16	theme	pH	873:874	arg1	measurements					876:887	The in vivo pH measurements	861:887	The in vivo pH measurements	861:887	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	6	17	from	application	959:969	arg1	teeth					982:986	carious teeth	974:986	carious teeth	974:986	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	5	18	theme	bicarbonate	828:838	arg1	potassium					805:813	potassium	805:813	potassium	805:813	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	18	theme	bicarbonate	828:838	arg1	amounts					794:800	higher amounts	787:800	higher amounts of potassium, calcium and bicarbonate	787:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	18	theme	bicarbonate	828:838	arg1	bicarbonate					828:838	bicarbonate	828:838	bicarbonate	828:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	18	theme	bicarbonate	828:838	arg1	calcium					816:822	calcium	816:822	calcium	816:822	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	9	19	theme	human	1278:1282	arg1	teeth					1284:1288	human teeth	1278:1288	human teeth	1278:1288	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	7	20	theme	saliva	1108:1113	arg1	composition					1069:1079	the composition	1065:1079	the composition of equine saliva and human saliva	1065:1113	CONCLUSIONS No large differences were found between the composition of equine saliva and human saliva.					
32446309	5	21	theme	potassium	805:813	arg1	potassium					805:813	potassium	805:813	potassium	805:813	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	21	theme	potassium	805:813	arg1	amounts					794:800	higher amounts	787:800	higher amounts of potassium, calcium and bicarbonate	787:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	21	theme	potassium	805:813	arg1	bicarbonate					828:838	bicarbonate	828:838	bicarbonate	828:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	21	theme	potassium	805:813	arg1	calcium					816:822	calcium	816:822	calcium	816:822	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	6	22	theme	resting	904:910	arg1	pH					912:913	a lower resting pH	896:913	a lower resting pH	896:913	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	2	23	theme	sucrose	421:427	arg1	application					429:439	sucrose application	421:439	sucrose application	421:439	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	0	24	from	mastication	32:42	arg1	biofilm					80:86	the oral biofilm	71:86	the oral biofilm of sound and carious tooth surfaces	71:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	7	25	theme	large	1028:1032	arg1	differences					1034:1044	No large differences	1025:1044	No large differences	1025:1044	CONCLUSIONS No large differences were found between the composition of equine saliva and human saliva.					
32446309	2	26	theme	cheek	470:474	arg1	teeth					476:480	vivo in sound and carious cheek teeth	444:480	teeth	476:480	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	3	27	theme	equine	524:529	arg1	concentrations					550:563	the equine saliva electrolyte concentrations	520:563	the equine saliva electrolyte concentrations	520:563	RESULTS Forage type had no effect on the equine saliva electrolyte concentrations, which varied considerably both intra- and inter-individually.					
32446309	0	28	theme	surfaces	115:122	arg1	biofilm					80:86	the oral biofilm	71:86	the oral biofilm of sound and carious tooth surfaces	71:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	0	29	theme	Equine	0:5	arg1	saliva					7:12	Equine saliva	0:12	Equine saliva	0:12	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	1	30	theme	equine	249:254	arg1	teeth					262:266	equine cheek teeth	249:266	equine cheek teeth	249:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	3	31	theme	saliva	531:536	arg1	concentrations					550:563	the equine saliva electrolyte concentrations	520:563	the equine saliva electrolyte concentrations	520:563	RESULTS Forage type had no effect on the equine saliva electrolyte concentrations, which varied considerably both intra- and inter-individually.					
32446309	4	32	theme	increased	648:656	arg1	values					658:663	increased values	648:663	increased values for all electrolytes except bicarbonate	648:703	Chewing resulted in increased values for all electrolytes except bicarbonate.					
32446309	1	33	theme	cheek	256:260	arg1	teeth					262:266	equine cheek teeth	249:266	equine cheek teeth	249:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	5	34	dep	phosphate	850:858	arg1	less					845:848	less	845:848	less	845:848	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	35	theme	higher	787:792	arg1	potassium					805:813	potassium	805:813	potassium	805:813	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	35	theme	higher	787:792	arg1	amounts					794:800	higher amounts	787:800	higher amounts of potassium, calcium and bicarbonate	787:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	35	theme	higher	787:792	arg1	bicarbonate					828:838	bicarbonate	828:838	bicarbonate	828:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	35	theme	higher	787:792	arg1	calcium					816:822	calcium	816:822	calcium	816:822	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	2	36	theme	measured	395:402	arg1	changes					407:413	measured pH changes	395:413	measured pH changes after sucrose application in vivo in sound and carious cheek teeth	395:480	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	2	37	theme	pH	404:405	arg1	changes					407:413	measured pH changes	395:413	measured pH changes after sucrose application in vivo in sound and carious cheek teeth	395:480	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	6	38	theme	pH	937:938	arg1	drop					940:943	a more pronounced pH drop	919:943	a more pronounced pH drop	919:943	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	0	39	theme	sound	91:95	arg1	surfaces					115:122	sound and carious tooth surfaces	91:122	sound and carious tooth surfaces	91:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	1	40	theme	saliva	171:176	arg1	composition					178:188	saliva composition	171:188	saliva composition	171:188	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	2	41	theme	in	449:450	arg1	sound					452:456	vivo in sound and carious cheek teeth	444:480	sound	452:456	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	6	42	theme	pronounced	926:935	arg1	drop					940:943	a more pronounced pH drop	919:943	a more pronounced pH drop	919:943	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	0	43	theme	tooth	109:113	arg1	surfaces					115:122	sound and carious tooth surfaces	91:122	sound and carious tooth surfaces	91:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	0	44	from	changes	60:66	arg1	biofilm					80:86	the oral biofilm	71:86	the oral biofilm of sound and carious tooth surfaces	71:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	0	45	dep	in	49:50	arg1	vivo					52:55	vivo	52:55	vivo	52:55	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	6	46	dep	in	865:866	arg1	vivo					868:871	vivo	868:871	vivo	868:871	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	1	47	theme	composition	178:188	arg1	role					163:166	The role	159:166	The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth	159:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	2	48	theme	vivo	444:447	arg1	sound					452:456	vivo in sound and carious cheek teeth	444:480	sound	452:456	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	3	49	contain	had	503:505	arg1	type					498:501	type	498:501	type	498:501	RESULTS Forage type had no effect on the equine saliva electrolyte concentrations, which varied considerably both intra- and inter-individually.					
32446309	3	49	contain	had	503:505	arg2	effect					510:515	no effect	507:515	no effect	507:515	RESULTS Forage type had no effect on the equine saliva electrolyte concentrations, which varied considerably both intra- and inter-individually.					
32446309	0	50	theme	carious	101:107	arg1	surfaces					115:122	sound and carious tooth surfaces	91:122	sound and carious tooth surfaces	91:122	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	9	51	theme	caries	1202:1207	arg1	process					1209:1215	the caries process	1198:1215	the caries process in equine cheek teeth	1198:1237	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	0	52	theme	in	49:50	arg1	changes					60:66	in vivo pH changes	49:66	in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure	49:145	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	5	53	theme	stimulated	720:729	arg1	saliva					737:742	stimulated human saliva	720:742	stimulated human saliva	720:742	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	8	54	theme	sound	1179:1183	arg1	teeth					1185:1189	sound teeth	1179:1189	sound teeth	1179:1189	A more pronounced acidogenicity was found for the carious than sound teeth.					
32446309	3	55	theme	electrolyte	538:548	arg1	concentrations					550:563	the equine saliva electrolyte concentrations	520:563	the equine saliva electrolyte concentrations	520:563	RESULTS Forage type had no effect on the equine saliva electrolyte concentrations, which varied considerably both intra- and inter-individually.					
32446309	2	56	theme	electrolyte	313:323	arg1	concentrations					334:347	electrolyte and urea concentrations	313:347	concentrations	334:347	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	5	57	dep	saliva	751:756	arg1	mastication					764:774	mastication	764:774	mastication	764:774	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	1	58	from	development	211:221	arg1	teeth					262:266	equine cheek teeth	249:266	equine cheek teeth	249:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	1	59	dep	BACKGROUND	148:157	arg1	understood					281:290	understood	281:290	is not fully understood	268:290	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	9	60	from	process	1209:1215	arg1	teeth					1233:1237	equine cheek teeth	1220:1237	equine cheek teeth	1220:1237	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	9	61	theme	equine	1220:1225	arg1	teeth					1233:1237	equine cheek teeth	1220:1237	equine cheek teeth	1220:1237	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	9	62	from	pattern	1264:1270	arg1	teeth					1284:1288	human teeth	1278:1288	human teeth	1278:1288	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	9	63	theme	oral	1320:1323	arg1	microorganisms					1325:1338	oral microorganisms	1320:1338	oral microorganisms	1320:1338	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	1	64	theme	dietary	194:200	arg1	sugar					202:206	dietary sugar	194:206	dietary sugar	194:206	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	0	65	theme	sucrose	130:136	arg1	exposure					138:145	sucrose exposure	130:145	sucrose exposure	130:145	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	9	66	theme	cheek	1227:1231	arg1	teeth					1233:1237	equine cheek teeth	1220:1237	equine cheek teeth	1220:1237	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	1	67	theme	sugar	202:206	arg1	role					163:166	The role	159:166	The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth	159:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	0	68	theme	pH	57:58	arg1	changes					60:66	in vivo pH changes	49:66	in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure	49:145	Equine saliva components during mastication, and in vivo pH changes in the oral biofilm of sound and carious tooth surfaces after sucrose exposure.					
32446309	1	69	from	role	163:166	arg1	development					211:221	development	211:221	development of infundibular caries in equine cheek teeth	211:266	BACKGROUND The role of saliva composition and dietary sugar in development of infundibular caries in equine cheek teeth is not fully understood.					
32446309	2	70	theme	carious	462:468	arg1	teeth					476:480	vivo in sound and carious cheek teeth	444:480	teeth	476:480	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	2	71	from	changes	407:413	arg1	sound					452:456	vivo in sound and carious cheek teeth	444:480	sound	452:456	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	2	71	from	changes	407:413	arg1	teeth					476:480	vivo in sound and carious cheek teeth	444:480	teeth	476:480	This study analysed electrolyte and urea concentrations in saliva in relation to different forage and measured pH changes after sucrose application in vivo in sound and carious cheek teeth.					
32446309	5	72	theme	calcium	816:822	arg1	potassium					805:813	potassium	805:813	potassium	805:813	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	72	theme	calcium	816:822	arg1	amounts					794:800	higher amounts	787:800	higher amounts of potassium, calcium and bicarbonate	787:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	72	theme	calcium	816:822	arg1	bicarbonate					828:838	bicarbonate	828:838	bicarbonate	828:838	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	5	72	theme	calcium	816:822	arg1	calcium					816:822	calcium	816:822	calcium	816:822	Compared with stimulated human saliva, horse saliva after mastication, contained higher amounts of potassium, calcium and bicarbonate, and less phosphate.					
32446309	6	73	theme	sound	1000:1004	arg1	teeth					1006:1010	sound teeth	1000:1010	sound teeth	1000:1010	The in vivo pH measurements showed a lower resting pH and a more pronounced pH drop after sucrose application in carious teeth compared to sound teeth.					
32446309	9	74	theme	sugar	1346:1350	arg1	consumption					1352:1362	sugar consumption	1346:1362	sugar consumption	1346:1362	Thus, the caries process in equine cheek teeth seems to follow the same pattern as in human teeth, caused by acid production by oral microorganisms after sugar consumption.					
32446309	8	75	theme	pronounced	1123:1132	arg1	acidogenicity					1134:1146	A more pronounced acidogenicity	1116:1146	A more pronounced acidogenicity	1116:1146	A more pronounced acidogenicity was found for the carious than sound teeth.					
32434125	0	0	theme	yeasts	96:101	arg1	inoculation					41:51	simultaneous inoculation	28:51	simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts	28:101	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	1	from	influence	15:23	arg1	volatiles					106:114	volatiles	106:114	volatiles	106:114	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	1	from	influence	15:23	arg1	acids					123:127	amino acids	117:127	amino acids	117:127	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	1	from	influence	15:23	arg1	amines					139:144	biogenic amines	130:144	biogenic amines	130:144	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	1	from	influence	15:23	arg1	profile					158:164	sensory profile	150:164	sensory profile	150:164	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	2	theme	wine	173:176	arg1	volatiles					106:114	volatiles	106:114	volatiles	106:114	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	2	theme	wine	173:176	arg1	amines					139:144	biogenic amines	130:144	biogenic amines	130:144	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	2	theme	wine	173:176	arg1	profile					158:164	sensory profile	150:164	sensory profile	150:164	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	3	with	profile	158:164	arg1	addition					192:199	lysozyme addition	183:199	lysozyme addition	183:199	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	1	4	theme	inoculation	233:243	arg1	timing					223:228	the timing	219:228	the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF))	219:331	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	7	5	theme	trained	1133:1139	arg1	panel					1141:1145	A trained panel	1131:1145	A trained panel	1131:1145	A trained panel did not perceive substantial differences between treatments.					
32434125	0	6	theme	red	169:171	arg1	wine					173:176	red wine	169:176	red wine	169:176	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	2	7	theme	SiO2-alginate	454:466	arg1	hydrogel					468:475	SiO2-alginate hydrogel	454:475	SiO2-alginate hydrogel (Si-ALG)	454:484	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	2	7	theme	SiO2-alginate	454:466	arg1	Si-ALG					478:483	Si-ALG	478:483	Si-ALG	478:483	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	5	8	theme	amine	1012:1016	arg1	composition					1018:1028	the biogenic amine composition	999:1028	the biogenic amine composition	999:1028	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32434125	4	9	theme	volatile	818:825	arg1	terpenes					863:870	terpenes	863:870	terpenes	863:870	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	4	9	theme	volatile	818:825	arg1	esters					852:857	ethyl esters	846:857	ethyl esters	846:857	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	4	9	theme	volatile	818:825	arg1	compounds					827:835	some volatile compounds	813:835	some volatile compounds	813:835	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	3	10	theme	volatile	652:659	arg1	composition					661:671	the volatile composition	648:671	the volatile composition of the wines	648:684	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	0	11	theme	lysozyme	183:190	arg1	addition					192:199	lysozyme addition	183:199	lysozyme addition	183:199	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	1	12	theme	sequential	246:255	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	12	theme	sequential	246:255	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	3	13	from	influence	606:614	arg1	composition					661:671	the volatile composition	648:671	the volatile composition of the wines	648:684	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	3	13	from	influence	606:614	arg1	acid					707:710	the amino acid	697:710	the amino acid	697:710	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	3	13	from	influence	606:614	arg1	content					731:737	biogenic amine content	716:737	biogenic amine content	716:737	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	4	14	theme	compounds	827:835	arg1	contents					801:808	the highest contents	789:808	the highest contents of some volatile compounds, such as ethyl esters and terpenes	789:870	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	4	14	theme	compounds	827:835	arg1	tyramine					900:907	tyramine	900:907	tyramine	900:907	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	4	14	theme	compounds	827:835	arg1	acids					890:894	amino acids	884:894	amino acids	884:894	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	0	15	theme	amino	117:121	arg1	volatiles					106:114	volatiles	106:114	volatiles	106:114	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	15	theme	amino	117:121	arg1	acids					123:127	amino acids	117:127	amino acids	117:127	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	3	16	theme	timing	623:628	arg1	influence					606:614	a significant influence	592:614	a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content	592:737	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	4	17	theme	highest	793:799	arg1	contents					801:808	the highest contents	789:808	the highest contents of some volatile compounds, such as ethyl esters and terpenes	789:870	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	2	18	theme	encapsulation	416:428	arg1	impact					402:407	The impact	398:407	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria	398:560	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	1	19	theme	chemical	340:347	arg1	properties					361:370	the chemical and sensory properties	336:370	the chemical and sensory properties of red wines	336:383	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	2	20	theme	encapsulated	540:551	arg1	bacteria					553:560	encapsulated bacteria	540:560	encapsulated bacteria	540:560	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	3	21	dep	just	686:689	arg1	as					691:692	as	691:692	as	691:692	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	2	22	theme	Oenococcus	433:442	arg1	addition					494:501	the addition	490:501	the addition of lysozyme	490:513	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	2	22	theme	Oenococcus	433:442	arg1	encapsulation					416:428	the encapsulation	412:428	the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG)	412:484	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	3	23	theme	wines	680:684	arg1	composition					661:671	the volatile composition	648:671	the volatile composition of the wines	648:684	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	3	24	theme	amino	701:705	arg1	acid					707:710	the amino acid	697:710	the amino acid	697:710	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	1	25	theme	simultaneous	261:272	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	25	theme	simultaneous	261:272	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	3	26	theme	inoculation	633:643	arg1	timing					623:628	the timing	619:628	the timing of inoculation	619:643	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	1	27	theme	alcoholic	274:282	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	27	theme	alcoholic	274:282	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	6	28	theme	wines	1080:1084	arg1	composition					1061:1071	The chemical composition	1048:1071	The chemical composition of the wines	1048:1084	The chemical composition of the wines was not altered by the presence of lysozyme.					
32434125	1	29	theme	fermentation	284:295	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	29	theme	fermentation	284:295	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	2	30	theme	lysozyme	506:513	arg1	addition					494:501	the addition	490:501	the addition of lysozyme	490:513	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	2	30	theme	lysozyme	506:513	arg1	encapsulation					416:428	the encapsulation	412:428	the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG)	412:484	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	1	31	theme	sensory	353:359	arg1	properties					361:370	the chemical and sensory properties	336:370	the chemical and sensory properties of red wines	336:383	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	0	32	theme	inoculation	41:51	arg1	influence					15:23	the influence	11:23	the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition	11:199	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	6	33	theme	chemical	1052:1059	arg1	composition					1061:1071	The chemical composition	1048:1071	The chemical composition of the wines	1048:1084	The chemical composition of the wines was not altered by the presence of lysozyme.					
32434125	4	34	theme	simultaneous	762:773	arg1	AF/MLF					775:780	simultaneous AF/MLF	762:780	simultaneous AF/MLF	762:780	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	0	35	theme	simultaneous	28:39	arg1	inoculation					41:51	simultaneous inoculation	28:51	simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts	28:101	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	1	36	theme	AF	298:299	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	36	theme	AF	298:299	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	0	37	theme	biogenic	130:137	arg1	volatiles					106:114	volatiles	106:114	volatiles	106:114	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	37	theme	biogenic	130:137	arg1	amines					139:144	biogenic amines	130:144	biogenic amines	130:144	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	3	38	theme	significant	594:604	arg1	influence					606:614	a significant influence	592:614	a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content	592:737	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	5	39	theme	amino	960:964	arg1	profile					971:977	the volatile and amino acid profile	943:977	profile	971:977	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32434125	4	40	theme	amino	884:888	arg1	acids					890:894	amino acids	884:894	amino acids	884:894	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	0	41	theme	SiO2-alginate	56:68	arg1	bacteria					83:90	SiO2-alginate encapsulated bacteria	56:90	SiO2-alginate encapsulated bacteria	56:90	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	3	42	theme	amine	725:729	arg1	content					731:737	biogenic amine content	716:737	biogenic amine content	716:737	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	0	43	theme	sensory	150:156	arg1	volatiles					106:114	volatiles	106:114	volatiles	106:114	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	0	43	theme	sensory	150:156	arg1	profile					158:164	sensory profile	150:164	sensory profile	150:164	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	1	44	theme	red	375:377	arg1	wines					379:383	red wines	375:383	red wines	375:383	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	5	45	theme	acid	966:969	arg1	profile					971:977	the volatile and amino acid profile	943:977	profile	971:977	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32434125	1	46	from	influence	206:214	arg1	properties					361:370	the chemical and sensory properties	336:370	the chemical and sensory properties of red wines	336:383	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	2	47	theme	addition	494:501	arg1	impact					402:407	The impact	398:407	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria	398:560	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	1	48	theme	/malolactic	301:311	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	48	theme	/malolactic	301:311	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	49	theme	wines	379:383	arg1	properties					361:370	the chemical and sensory properties	336:370	the chemical and sensory properties of red wines	336:383	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	5	50	theme	biogenic	1003:1010	arg1	composition					1018:1028	the biogenic amine composition	999:1028	the biogenic amine composition	999:1028	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32434125	0	51	with	amines	139:144	arg1	addition					192:199	lysozyme addition	183:199	lysozyme addition	183:199	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	3	52	theme	biogenic	716:723	arg1	content					731:737	biogenic amine content	716:737	biogenic amine content	716:737	There was a significant influence of the timing of inoculation on the volatile composition of the wines just as on the amino acid and biogenic amine content.					
32434125	4	53	theme	ethyl	846:850	arg1	esters					852:857	ethyl esters	846:857	ethyl esters	846:857	The wines produced by simultaneous AF/MLF showed the highest contents of some volatile compounds, such as ethyl esters and terpenes, as well as amino acids and tyramine.					
32434125	1	54	dep	inoculation	233:243	arg1	MLF					327:329	MLF	327:329	MLF	327:329	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	1	54	dep	inoculation	233:243	arg1	fermentation					313:324	sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation	246:324	fermentation	313:324	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	6	55	theme	lysozyme	1121:1128	arg1	presence					1109:1116	the presence	1105:1116	the presence of lysozyme	1105:1128	The chemical composition of the wines was not altered by the presence of lysozyme.					
32434125	5	56	theme	wines	986:990	arg1	volatile					947:954	the volatile and amino acid profile	943:977	volatile	947:954	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32434125	5	56	theme	wines	986:990	arg1	profile					971:977	the volatile and amino acid profile	943:977	profile	971:977	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32434125	0	57	theme	bacteria	83:90	arg1	inoculation					41:51	simultaneous inoculation	28:51	simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts	28:101	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	7	58	theme	substantial	1164:1174	arg1	differences					1176:1186	substantial differences	1164:1186	substantial differences between treatments	1164:1205	A trained panel did not perceive substantial differences between treatments.					
32434125	1	59	theme	timing	223:228	arg1	influence					206:214	The influence	202:214	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines	202:383	The influence of the timing of inoculation (sequential and simultaneous alcoholic fermentation (AF)/malolactic fermentation (MLF)) on the chemical and sensory properties of red wines was studied.					
32434125	0	60	theme	encapsulated	70:81	arg1	bacteria					83:90	SiO2-alginate encapsulated bacteria	56:90	SiO2-alginate encapsulated bacteria	56:90	Evaluating the influence of simultaneous inoculation of SiO2-alginate encapsulated bacteria and yeasts on volatiles, amino acids, biogenic amines and sensory profile of red wine with lysozyme addition.					
32434125	2	61	from	impact	402:407	arg1	wines					518:522	wines	518:522	wines inoculated with encapsulated bacteria	518:560	The impact of the encapsulation of Oenococcus oeni into SiO2-alginate hydrogel (Si-ALG) and the addition of lysozyme in wines inoculated with encapsulated bacteria were also analysed.					
32434125	5	62	theme	Bacterial	910:918	arg1	encapsulation					920:932	Bacterial encapsulation	910:932	Bacterial encapsulation	910:932	Bacterial encapsulation affected the volatile and amino acid profile of the wines, while the biogenic amine composition was not modified.					
32271330	8	0	theme	energy	1373:1378	arg1	source					1380:1385	an important energy source	1360:1385	an important energy source for exercise	1360:1398	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	8	0	theme	energy	1373:1378	arg1	content					1351:1357	Tissue glycogen content	1335:1357	Tissue glycogen content	1335:1357	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	9	1	theme	microbiota	1539:1548	arg1	composition					1550:1560	the gut microbiota composition	1531:1560	the gut microbiota composition	1531:1560	CONCLUSION Taken together, our results indicate that NCE supplementation alters the gut microbiota composition and aids in overcoming physical fatigue.					
32271330	6	2	theme	forelimb	1018:1025	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	1	3	theme	exercise-related	245:260	arg1	injuries					262:269	exercise-related injuries	245:269	exercise-related injuries	245:269	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	0	4	theme	exercise	88:95	arg1	performance					97:107	exercise performance	88:107	exercise performance	88:107	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	3	5	theme	g	613:613	arg1	day-1					620:624	15.375 g kg-1 day-1	606:624	15.375 g kg-1 day-1 (NCE-5X)	606:633	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	3	5	theme	g	613:613	arg1	NCE-5X					627:632	NCE-5X	627:632	NCE-5X	627:632	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	9	6	theme	physical	1585:1592	arg1	fatigue					1594:1600	physical fatigue	1585:1600	physical fatigue	1585:1600	CONCLUSION Taken together, our results indicate that NCE supplementation alters the gut microbiota composition and aids in overcoming physical fatigue.					
32271330	2	7	dep	METHODS	298:304	arg1	seek					324:327	seek	324:327	seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance	324:456	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	3	8	theme	kg-1	615:618	arg1	day-1					620:624	15.375 g kg-1 day-1	606:624	15.375 g kg-1 day-1 (NCE-5X)	606:633	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	3	8	theme	kg-1	615:618	arg1	NCE-5X					627:632	NCE-5X	627:632	NCE-5X	627:632	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	7	9	theme	exhaustive	1243:1252	arg1	time					1263:1266	a significantly longer exhaustive swimming time	1220:1266	a significantly longer exhaustive swimming time	1220:1266	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	3	10	theme	=	503:503	arg1	n					501:501	n = 8	501:505	n = 8	501:505	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	6	11	theme	urea	1094:1097	arg1	BUN					1109:1111	BUN	1109:1111	BUN	1109:1111	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	11	theme	urea	1094:1097	arg1	nitrogen					1099:1106	blood urea nitrogen	1088:1106	blood urea nitrogen (BUN)	1088:1112	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	12	theme	lactate	1061:1067	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	12	theme	lactate	1061:1067	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	12	theme	lactate	1061:1067	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	7	13	theme	forelimb	1288:1295	arg1	strength					1302:1309	higher relative forelimb grip strength	1272:1309	higher relative forelimb grip strength	1272:1309	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	6	14	theme	ammonia	1070:1076	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	14	theme	ammonia	1070:1076	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	14	theme	ammonia	1070:1076	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	15	theme	blood	1088:1092	arg1	BUN					1109:1111	BUN	1109:1111	BUN	1109:1111	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	15	theme	blood	1088:1092	arg1	nitrogen					1099:1106	blood urea nitrogen	1088:1106	blood urea nitrogen (BUN)	1088:1112	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	2	16	from	changes	344:350	arg1	composition					374:384	the gut microbiota composition	355:384	the gut microbiota composition	355:384	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	5	17	theme	vehicle	881:887	arg1	group					889:893	the vehicle group	877:893	the vehicle group	877:893	RESULTS NCE-5X did not appear to obviously cluster with the vehicle group, although NCE-5X groups showed an increased Firmicutes/Bacteroidetes ratio compared with the vehicle group.					
32271330	5	18	theme	increased	822:830	arg1	ratio					857:861	an increased Firmicutes/Bacteroidetes ratio	819:861	an increased Firmicutes/Bacteroidetes ratio	819:861	RESULTS NCE-5X did not appear to obviously cluster with the vehicle group, although NCE-5X groups showed an increased Firmicutes/Bacteroidetes ratio compared with the vehicle group.					
32271330	3	19	from	3.075	587:591	arg1	weeks					565:569	six weeks	561:569	six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X)	561:633	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	6	20	theme	lactate	1140:1146	arg1	LDH					1163:1165	LDH	1163:1165	LDH	1163:1165	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	20	theme	lactate	1140:1146	arg1	dehydrogenase					1148:1160	lactate dehydrogenase	1140:1160	lactate dehydrogenase (LDH)	1140:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	4	21	from	mice	664:667	arg1	microbiota					644:653	The gut microbiota	636:653	The gut microbiota from the mice	636:667	The gut microbiota from the mice was analyzed using 16S rRNA gene sequencing.					
32271330	3	22	from	0	574:574	arg1	weeks					565:569	six weeks	561:569	six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X)	561:633	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	1	23	theme	potential	167:175	arg1	effect					188:193	the potential beneficial effect	163:193	the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance	163:295	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	6	24	theme	anti-fatigue	909:920	arg1	activity					922:929	anti-fatigue activity	909:929	anti-fatigue activity	909:929	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	3	25	dep	groups	493:498	arg1	n					501:501	n = 8	501:505	n = 8	501:505	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	2	26	theme	NCE	391:393	arg1	supplementation					395:409	NCE supplementation	391:409	NCE supplementation in relation to health and exercise performance	391:456	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	6	27	theme	dehydrogenase	1148:1160	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	27	theme	dehydrogenase	1148:1160	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	27	theme	dehydrogenase	1148:1160	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	5	28	theme	Firmicutes/Bacteroidetes	832:855	arg1	ratio					857:861	an increased Firmicutes/Bacteroidetes ratio	819:861	an increased Firmicutes/Bacteroidetes ratio	819:861	RESULTS NCE-5X did not appear to obviously cluster with the vehicle group, although NCE-5X groups showed an increased Firmicutes/Bacteroidetes ratio compared with the vehicle group.					
32271330	6	29	theme	exhaustive	992:1001	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	5	30	theme	RESULTS	714:720	arg1	NCE-5X					722:727	RESULTS NCE-5X	714:727	RESULTS NCE-5X	714:727	RESULTS NCE-5X did not appear to obviously cluster with the vehicle group, although NCE-5X groups showed an increased Firmicutes/Bacteroidetes ratio compared with the vehicle group.					
32271330	4	31	theme	gut	640:642	arg1	microbiota					644:653	The gut microbiota	636:653	The gut microbiota from the mice	636:667	The gut microbiota from the mice was analyzed using 16S rRNA gene sequencing.					
32271330	7	32	theme	longer	1236:1241	arg1	time					1263:1266	a significantly longer exhaustive swimming time	1220:1266	a significantly longer exhaustive swimming time	1220:1266	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	5	33	theme	vehicle	774:780	arg1	group					782:786	the vehicle group	770:786	the vehicle group	770:786	RESULTS NCE-5X did not appear to obviously cluster with the vehicle group, although NCE-5X groups showed an increased Firmicutes/Bacteroidetes ratio compared with the vehicle group.					
32271330	2	34	theme	exercise	437:444	arg1	performance					446:456	exercise performance	437:456	exercise performance	437:456	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	6	35	theme	swimming	1003:1010	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	8	36	theme	Tissue	1335:1340	arg1	source					1380:1385	an important energy source	1360:1385	an important energy source for exercise	1360:1398	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	8	36	theme	Tissue	1335:1340	arg1	content					1351:1357	Tissue glycogen content	1335:1357	Tissue glycogen content	1335:1357	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	9	37	theme	NCE	1504:1506	arg1	supplementation					1508:1522	NCE supplementation	1504:1522	NCE supplementation	1504:1522	CONCLUSION Taken together, our results indicate that NCE supplementation alters the gut microbiota composition and aids in overcoming physical fatigue.					
32271330	7	38	theme	higher	1272:1277	arg1	strength					1302:1309	higher relative forelimb grip strength	1272:1309	higher relative forelimb grip strength	1272:1309	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	1	39	theme	nano-bubble	198:208	arg1	NCE					228:230	NCE	228:230	NCE	228:230	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	1	39	theme	nano-bubble	198:208	arg1	extract					219:225	nano-bubble curcumin extract	198:225	nano-bubble curcumin extract (NCE)	198:231	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	0	40	theme	nano-bubble	19:29	arg1	extract					40:46	nano-bubble curcumin extract	19:46	nano-bubble curcumin extract	19:46	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	10	41	theme	gut	1633:1635	arg1	microbiome					1637:1646	the gut microbiome	1629:1646	the gut microbiome to modulate the gut system towards improving exercise performance	1629:1712	Curcumin may be acting on the gut microbiome to modulate the gut system towards improving exercise performance.					
32271330	0	42	from	performance	97:107	arg1	mice					112:115	mice	112:115	mice	112:115	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	5	43	theme	NCE-5X	798:803	arg1	groups					805:810	NCE-5X groups	798:810	NCE-5X groups	798:810	RESULTS NCE-5X did not appear to obviously cluster with the vehicle group, although NCE-5X groups showed an increased Firmicutes/Bacteroidetes ratio compared with the vehicle group.					
32271330	1	44	theme	curcumin	210:217	arg1	NCE					228:230	NCE	228:230	NCE	228:230	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	1	44	theme	curcumin	210:217	arg1	extract					219:225	nano-bubble curcumin extract	198:225	nano-bubble curcumin extract (NCE)	198:231	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	3	45	theme	Male	459:462	arg1	mice					468:471	Male ICR mice	459:471	Male ICR mice	459:471	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	10	46	theme	exercise	1693:1700	arg1	performance					1702:1712	exercise performance	1693:1712	exercise performance	1693:1712	Curcumin may be acting on the gut microbiome to modulate the gut system towards improving exercise performance.					
32271330	7	47	theme	vehicle	1320:1326	arg1	group					1328:1332	the vehicle group	1316:1332	the vehicle group	1316:1332	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	1	48	theme	extract	219:225	arg1	effect					188:193	the potential beneficial effect	163:193	the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance	163:295	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	0	49	theme	extract	40:46	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of nano-bubble curcumin extract	0:46	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	7	50	theme	NCE-5X	1199:1204	arg1	groups					1206:1211	The NCE-1X and NCE-5X groups	1184:1211	The NCE-1X and NCE-5X groups	1184:1211	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	0	51	theme	curcumin	31:38	arg1	extract					40:46	nano-bubble curcumin extract	19:46	nano-bubble curcumin extract	19:46	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	3	52	theme	ICR	464:466	arg1	mice					468:471	Male ICR mice	459:471	Male ICR mice	459:471	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	0	53	theme	microbiota	61:70	arg1	composition					72:82	gut microbiota composition	57:82	gut microbiota composition	57:82	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	0	54	from	composition	72:82	arg1	mice					112:115	mice	112:115	mice	112:115	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	6	55	theme	creatine	1115:1122	arg1	CK					1132:1133	CK	1132:1133	CK	1132:1133	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	55	theme	creatine	1115:1122	arg1	kinase					1124:1129	creatine kinase	1115:1129	creatine kinase (CK)	1115:1134	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	0	56	theme	gut	57:59	arg1	composition					72:82	gut microbiota composition	57:82	gut microbiota composition	57:82	Supplementation of nano-bubble curcumin extract improves gut microbiota composition and exercise performance in mice.					
32271330	6	57	theme	kinase	1124:1129	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	57	theme	kinase	1124:1129	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	57	theme	kinase	1124:1129	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	7	58	theme	swimming	1254:1261	arg1	time					1263:1266	a significantly longer exhaustive swimming time	1220:1266	a significantly longer exhaustive swimming time	1220:1266	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	2	59	from	supplementation	395:409	arg1	relation					414:421	relation	414:421	relation to health and exercise performance	414:456	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	8	60	theme	glycogen	1342:1349	arg1	source					1380:1385	an important energy source	1360:1385	an important energy source for exercise	1360:1398	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	8	60	theme	glycogen	1342:1349	arg1	content					1351:1357	Tissue glycogen content	1335:1357	Tissue glycogen content	1335:1357	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	10	61	theme	gut	1664:1666	arg1	system					1668:1673	the gut system	1660:1673	the gut system	1660:1673	Curcumin may be acting on the gut microbiome to modulate the gut system towards improving exercise performance.					
32271330	6	62	theme	nitrogen	1099:1106	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	62	theme	nitrogen	1099:1106	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	62	theme	nitrogen	1099:1106	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	4	63	theme	rRNA	692:695	arg1	sequencing					702:711	16S rRNA gene sequencing	688:711	16S rRNA gene sequencing	688:711	The gut microbiota from the mice was analyzed using 16S rRNA gene sequencing.					
32271330	6	64	theme	exercise	935:942	arg1	performance					944:954	exercise performance	935:954	exercise performance	935:954	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	1	65	theme	previous	134:141	arg1	study					143:147	a previous study	132:147	a previous study	132:147	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	7	66	theme	relative	1279:1286	arg1	strength					1302:1309	higher relative forelimb grip strength	1272:1309	higher relative forelimb grip strength	1272:1309	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	6	67	theme	glucose	1079:1085	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	67	theme	glucose	1079:1085	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	67	theme	glucose	1079:1085	arg1	time					1012:1015	the exhaustive swimming time	988:1015	the exhaustive swimming time	988:1015	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	8	68	theme	important	1363:1371	arg1	source					1380:1385	an important energy source	1360:1385	an important energy source for exercise	1360:1398	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	8	68	theme	important	1363:1371	arg1	content					1351:1357	Tissue glycogen content	1335:1357	Tissue glycogen content	1335:1357	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	4	69	theme	16S	688:690	arg1	sequencing					702:711	16S rRNA gene sequencing	688:711	16S rRNA gene sequencing	688:711	The gut microbiota from the mice was analyzed using 16S rRNA gene sequencing.					
32271330	7	70	theme	grip	1297:1300	arg1	strength					1302:1309	higher relative forelimb grip strength	1272:1309	higher relative forelimb grip strength	1272:1309	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	1	71	dep	BACKGROUND	118:127	arg1	evaluated					153:161	evaluated	153:161	evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance	153:295	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	7	72	theme	NCE-1X	1188:1193	arg1	groups					1206:1211	The NCE-1X and NCE-5X groups	1184:1211	The NCE-1X and NCE-5X groups	1184:1211	The NCE-1X and NCE-5X groups showed a significantly longer exhaustive swimming time and higher relative forelimb grip strength than the vehicle group.					
32271330	6	73	theme	serum	1045:1049	arg1	levels					1051:1056	serum levels	1045:1056	serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH)	1045:1166	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	6	74	theme	grip	1027:1030	arg1	strength					1032:1039	forelimb grip strength	1018:1039	forelimb grip strength	1018:1039	In addition, anti-fatigue activity and exercise performance were evaluated by investigating the exhaustive swimming time, forelimb grip strength and serum levels of lactate, ammonia, glucose, blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) after swimming.					
32271330	8	75	theme	NCE	1430:1432	arg1	supplementation					1434:1448	NCE supplementation	1430:1448	NCE supplementation	1430:1448	Tissue glycogen content, an important energy source for exercise, increased significantly with NCE supplementation.					
32271330	2	76	theme	microbiota	363:372	arg1	composition					374:384	the gut microbiota composition	355:384	the gut microbiota composition	355:384	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	1	77	theme	beneficial	177:186	arg1	effect					188:193	the potential beneficial effect	163:193	the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance	163:295	BACKGROUND In a previous study, we evaluated the potential beneficial effect of nano-bubble curcumin extract (NCE) in reducing exercise-related injuries and improving performance.					
32271330	3	78	from	day-1	620:624	arg1	weeks					565:569	six weeks	561:569	six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X)	561:633	Male ICR mice were divided into 3 groups (n = 8 per group) and orally administered NCE once daily for six weeks at 0 (vehicle), 3.075 (NCE-1X) and 15.375 g kg-1 day-1 (NCE-5X).					
32271330	4	79	theme	gene	697:700	arg1	sequencing					702:711	16S rRNA gene sequencing	688:711	16S rRNA gene sequencing	688:711	The gut microbiota from the mice was analyzed using 16S rRNA gene sequencing.					
32271330	2	80	theme	gut	359:361	arg1	composition					374:384	the gut microbiota composition	355:384	the gut microbiota composition	355:384	METHODS In this study, we seek to investigate changes in the gut microbiota composition upon NCE supplementation in relation to health and exercise performance.					
32271330	9	81	theme	gut	1535:1537	arg1	composition					1550:1560	the gut microbiota composition	1531:1560	the gut microbiota composition	1531:1560	CONCLUSION Taken together, our results indicate that NCE supplementation alters the gut microbiota composition and aids in overcoming physical fatigue.					
33302781	5	0	theme	lipids/COD	1057:1066	arg1	yield					1032:1036	conversion yield	1021:1036	conversion yield of biomass/COD and lipids/COD	1021:1066	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	4	1	from	biomass	776:782	arg1	CEW					818:820	CEW	818:820	CEW	818:820	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	5	2	theme	conversion	1021:1030	arg1	yield					1032:1036	conversion yield	1021:1036	conversion yield of biomass/COD and lipids/COD	1021:1066	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	5	3	from	%	992:992	arg1	terms					1012:1016	terms	1012:1016	terms of conversion yield of biomass/COD and lipids/COD	1012:1066	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	0	4	from	study	12:16	arg1	fermentation					62:73	cellulosic ethanol fermentation	43:73	cellulosic ethanol fermentation	43:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	6	5	dep	obtained	1154:1161	arg1	%					1247:1247	57.64%	1242:1247	57.64%	1242:1247	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	2	6	theme	lipid	525:529	arg1	content					531:537	lipid content	525:537	lipid content	525:537	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	7	dep	yeasts	332:337	arg1	yeasts					332:337	four oleaginous yeasts	316:337	four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme)	316:430	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	7	dep	yeasts	332:337	arg1	cutaneum					353:360	cutaneum	353:360	cutaneum	353:360	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	7	dep	yeasts	332:337	arg1	toruloides					378:387	toruloides	378:387	toruloides	378:387	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	7	dep	yeasts	332:337	arg1	albidus					403:409	Cryptococcus albidus	390:409	Cryptococcus albidus	390:409	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	7	dep	yeasts	332:337	arg1	coremiiforme					418:429	coremiiforme	418:429	coremiiforme	418:429	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	0	8	theme	oleaginous	95:104	arg1	yeasts					106:111	four oleaginous yeasts	90:111	four oleaginous yeasts	90:111	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	3	9	theme	treatment	714:722	arg1	effect					692:697	the effect	688:697	the effect of wastewaters treatment	688:722	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	7	10	from	fermentation	1378:1389	arg1	potential					1297:1305	great potential	1291:1305	great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation	1291:1389	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	7	11	theme	wastewaters	1332:1342	arg1	treatment					1344:1352	wastewaters treatment	1332:1352	wastewaters treatment	1332:1352	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	7	12	contain	has	1287:1289	arg1	cutaneum					1278:1285	T. cutaneum	1275:1285	T. cutaneum	1275:1285	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	7	12	contain	has	1287:1289	arg2	potential					1297:1305	great potential	1291:1305	great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation	1291:1389	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	2	13	theme	production	513:522	arg1	terms					496:500	terms	496:500	terms of biomass production, lipid content and lipid yield	496:553	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	5	14	theme	yield	1032:1036	arg1	terms					1012:1016	terms	1012:1016	terms of conversion yield of biomass/COD and lipids/COD	1012:1066	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	6	15	theme	acid	1193:1196	arg1	content					1198:1204	the highest unsaturated fatty acid content	1163:1204	the highest unsaturated fatty acid content	1163:1204	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	6	15	theme	acid	1193:1196	arg1	content					1211:1217	the content	1207:1217	the content of C18:1 and C18:2	1207:1236	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	4	16	theme	highest	768:774	arg1	20.945					827:832	20.945	827:832	20.945	827:832	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	4	16	theme	highest	768:774	arg1	biomass					776:782	biomass	776:782	biomass	776:782	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	2	17	theme	biomass	505:511	arg1	production					513:522	biomass production	505:522	biomass production	505:522	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	7	18	theme	cellulosic	1359:1368	arg1	fermentation					1378:1389	cellulosic ethanol fermentation	1359:1389	cellulosic ethanol fermentation	1359:1389	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	6	19	theme	fatty	1187:1191	arg1	acid					1193:1196	unsaturated fatty acid	1175:1196	the highest unsaturated fatty acid content	1163:1204	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	5	20	theme	maximum	934:940	arg1	rates					953:957	The maximum conversion rates	930:957	The maximum conversion rates of T. cutaneum	930:972	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	5	20	theme	maximum	934:940	arg1	%					992:992	73.64 and 6.06%	978:992	73.64 and 6.06%	978:992	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	2	21	theme	Cryptococcus	390:401	arg1	yeasts					332:337	four oleaginous yeasts	316:337	four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme)	316:430	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	21	theme	Cryptococcus	390:401	arg1	albidus					403:409	Cryptococcus albidus	390:409	Cryptococcus albidus	390:409	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	3	22	theme	wastewaters	702:712	arg1	treatment					714:722	wastewaters treatment	702:722	wastewaters treatment	702:722	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	6	23	theme	unsaturated	1175:1185	arg1	acid					1193:1196	unsaturated fatty acid	1175:1196	the highest unsaturated fatty acid content	1163:1204	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	6	24	theme	acids	1090:1094	arg1	content					1073:1079	The content	1069:1079	The content of fatty acids	1069:1094	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	0	25	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of lipid production from cellulosic ethanol fermentation	0:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	6	26	theme	highest	1167:1173	arg1	content					1198:1204	the highest unsaturated fatty acid content	1163:1204	the highest unsaturated fatty acid content	1163:1204	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	6	26	theme	highest	1167:1173	arg1	content					1211:1217	the content	1207:1217	the content of C18:1 and C18:2	1207:1236	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	2	27	theme	wastewater	304:313	arg1	utilization					270:280	the utilization	266:280	the utilization of cellulosic ethanol wastewater	266:313	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	3	28	theme	fatty	638:642	arg1	composition					649:659	fatty acid composition	638:659	fatty acid composition	638:659	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	0	29	theme	lipid	21:25	arg1	production					27:36	lipid production	21:36	lipid production from cellulosic ethanol fermentation	21:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	2	30	theme	yield	549:553	arg1	terms					496:500	terms	496:500	terms of biomass production, lipid content and lipid yield	496:553	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	4	31	theme	cutaneum	806:813	arg1	20.945					827:832	20.945	827:832	20.945	827:832	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	4	31	theme	cutaneum	806:813	arg1	biomass					776:782	biomass	776:782	biomass	776:782	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	4	31	theme	cutaneum	806:813	arg1	yield					794:798	lipid yield	788:798	lipid yield	788:798	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	2	32	theme	ethanol	296:302	arg1	wastewater					304:313	cellulosic ethanol wastewater	285:313	cellulosic ethanol wastewater	285:313	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	3	33	theme	thechemical	569:579	arg1	rate					612:615	thechemical oxygen demand (COD) conversion rate	569:615	thechemical oxygen demand (COD) conversion rate	569:615	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	2	34	theme	lipid	543:547	arg1	yield					549:553	lipid yield	543:553	lipid yield	543:553	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	4	35	theme	highest	898:904	arg1	content					912:918	the highest lipid content	894:918	the highest lipid content (17.32%)	894:927	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	4	35	theme	highest	898:904	arg1	%					926:926	17.32%	921:926	17.32%	921:926	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	2	36	theme	cellulosic	285:294	arg1	wastewater					304:313	cellulosic ethanol wastewater	285:313	cellulosic ethanol wastewater	285:313	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	7	37	theme	lipid	1311:1315	arg1	production					1317:1326	lipid production	1311:1326	lipid production	1311:1326	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	6	38	theme	fatty	1084:1088	arg1	acids					1090:1094	fatty acids	1084:1094	fatty acids	1084:1094	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	2	39	theme	lipid	476:480	arg1	production					482:491	lipid production	476:491	lipid production in terms of biomass production, lipid content and lipid yield	476:553	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	4	40	theme	lipid	906:910	arg1	content					912:918	the highest lipid content	894:918	the highest lipid content (17.32%)	894:927	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	4	40	theme	lipid	906:910	arg1	%					926:926	17.32%	921:926	17.32%	921:926	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	2	41	from	production	482:491	arg1	terms					496:500	terms	496:500	terms of biomass production, lipid content and lipid yield	496:553	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	5	42	theme	cutaneum	965:972	arg1	rates					953:957	The maximum conversion rates	930:957	The maximum conversion rates of T. cutaneum	930:972	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	5	42	theme	cutaneum	965:972	arg1	%					992:992	73.64 and 6.06%	978:992	73.64 and 6.06%	978:992	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	1	43	theme	ideal	202:206	arg1	process					214:220	an ideal green process	199:220	an ideal green process	199:220	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	43	theme	ideal	202:206	arg1	treatment					130:138	The biochemical treatment	114:138	The biochemical treatment of cellulosic ethanol wastewater (CEW)	114:177	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	0	44	theme	production	27:36	arg1	study					12:16	Comparative study	0:16	Comparative study of lipid production from cellulosic ethanol fermentation	0:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	2	45	theme	content	531:537	arg1	terms					496:500	terms	496:500	terms of biomass production, lipid content and lipid yield	496:553	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	3	46	theme	COD	618:620	arg1	degradation					622:632	COD degradation	618:632	COD degradation	618:632	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	7	47	theme	ethanol	1370:1376	arg1	fermentation					1378:1389	cellulosic ethanol fermentation	1359:1389	cellulosic ethanol fermentation	1359:1389	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	1	48	theme	green	208:212	arg1	process					214:220	an ideal green process	199:220	an ideal green process	199:220	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	48	theme	green	208:212	arg1	treatment					130:138	The biochemical treatment	114:138	The biochemical treatment of cellulosic ethanol wastewater (CEW)	114:177	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	0	49	theme	ethanol	54:60	arg1	fermentation					62:73	cellulosic ethanol fermentation	43:73	cellulosic ethanol fermentation	43:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	6	50	theme	C18:2	1232:1236	arg1	content					1198:1204	the highest unsaturated fatty acid content	1163:1204	the highest unsaturated fatty acid content	1163:1204	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	6	50	theme	C18:2	1232:1236	arg1	content					1211:1217	the content	1207:1217	the content of C18:1 and C18:2	1207:1236	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	0	51	from	fermentation	62:73	arg1	study					12:16	Comparative study	0:16	Comparative study of lipid production from cellulosic ethanol fermentation	0:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	0	51	from	fermentation	62:73	arg1	production					27:36	lipid production	21:36	lipid production from cellulosic ethanol fermentation	21:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	3	52	theme	oxygen	581:586	arg1	COD					596:598	COD	596:598	COD	596:598	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	3	52	theme	oxygen	581:586	arg1	demand					588:593	oxygen demand	581:593	thechemical oxygen demand (COD) conversion rate	569:615	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	1	53	theme	biochemical	118:128	arg1	process					214:220	an ideal green process	199:220	an ideal green process	199:220	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	53	theme	biochemical	118:128	arg1	treatment					130:138	The biochemical treatment	114:138	The biochemical treatment of cellulosic ethanol wastewater (CEW)	114:177	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	0	54	theme	cellulosic	43:52	arg1	fermentation					62:73	cellulosic ethanol fermentation	43:73	cellulosic ethanol fermentation	43:73	Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts.					
33302781	5	55	theme	biomass/COD	1041:1051	arg1	yield					1032:1036	conversion yield	1021:1036	conversion yield of biomass/COD and lipids/COD	1021:1066	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	3	56	theme	demand	588:593	arg1	rate					612:615	thechemical oxygen demand (COD) conversion rate	569:615	thechemical oxygen demand (COD) conversion rate	569:615	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	6	57	theme	T.	1138:1139	arg1	coremiiforme					1141:1152	T. coremiiforme	1138:1152	T. coremiiforme	1138:1152	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	4	58	theme	lipid	788:792	arg1	yield					794:798	lipid yield	788:798	lipid yield	788:798	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	6	59	theme	C18:1	1222:1226	arg1	content					1198:1204	the highest unsaturated fatty acid content	1163:1204	the highest unsaturated fatty acid content	1163:1204	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	6	59	theme	C18:1	1222:1226	arg1	content					1211:1217	the content	1207:1217	the content of C18:1 and C18:2	1207:1236	The content of fatty acids showed that after six days' fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%.					
33302781	5	60	theme	conversion	942:951	arg1	rates					953:957	The maximum conversion rates	930:957	The maximum conversion rates of T. cutaneum	930:972	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	5	60	theme	conversion	942:951	arg1	%					992:992	73.64 and 6.06%	978:992	73.64 and 6.06%	978:992	The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD.					
33302781	2	61	theme	oleaginous	246:255	arg1	yeastfor					257:264	the best oleaginous yeastfor	237:264	the best oleaginous yeastfor	237:264	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	7	62	theme	great	1291:1295	arg1	potential					1297:1305	great potential	1291:1305	great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation	1291:1389	This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.					
33302781	2	63	theme	oleaginous	321:330	arg1	yeasts					332:337	four oleaginous yeasts	316:337	four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme)	316:430	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	63	theme	oleaginous	321:330	arg1	cutaneum					353:360	cutaneum	353:360	cutaneum	353:360	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	63	theme	oleaginous	321:330	arg1	toruloides					378:387	toruloides	378:387	toruloides	378:387	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	63	theme	oleaginous	321:330	arg1	albidus					403:409	Cryptococcus albidus	390:409	Cryptococcus albidus	390:409	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	2	63	theme	oleaginous	321:330	arg1	coremiiforme					418:429	coremiiforme	418:429	coremiiforme	418:429	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	4	64	theme	fermentation	742:753	arg1	results					755:761	the fermentation results	738:761	the fermentation results	738:761	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	4	65	theme	R.	872:873	arg1	toruloides					875:884	the R. toruloides	868:884	the R. toruloides	868:884	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	3	66	theme	acid	644:647	arg1	composition					649:659	fatty acid composition	638:659	fatty acid composition	638:659	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	2	67	theme	best	241:244	arg1	yeastfor					257:264	the best oleaginous yeastfor	237:264	the best oleaginous yeastfor	237:264	To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield.					
33302781	4	68	from	yield	794:798	arg1	CEW					818:820	CEW	818:820	CEW	818:820	According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%).					
33302781	1	69	theme	cellulosic	143:152	arg1	wastewater					162:171	cellulosic ethanol wastewater	143:171	cellulosic ethanol wastewater (CEW)	143:177	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	69	theme	cellulosic	143:152	arg1	CEW					174:176	CEW	174:176	CEW	174:176	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	70	theme	ethanol	154:160	arg1	wastewater					162:171	cellulosic ethanol wastewater	143:171	cellulosic ethanol wastewater (CEW)	143:177	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	70	theme	ethanol	154:160	arg1	CEW					174:176	CEW	174:176	CEW	174:176	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	3	71	theme	conversion	601:610	arg1	rate					612:615	thechemical oxygen demand (COD) conversion rate	569:615	thechemical oxygen demand (COD) conversion rate	569:615	Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment.					
33302781	1	72	theme	wastewater	162:171	arg1	process					214:220	an ideal green process	199:220	an ideal green process	199:220	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
33302781	1	72	theme	wastewater	162:171	arg1	treatment					130:138	The biochemical treatment	114:138	The biochemical treatment of cellulosic ethanol wastewater (CEW)	114:177	The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process.					
31972199	4	0	with	co-presence	669:679	arg1	weight					739:744	a calculated percentage weight	715:744	a calculated percentage weight of Ag	715:750	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	7	1	theme	pollutant	1045:1053	arg1	p-nitrophenol					1055:1067	anthropogenic pollutant p-nitrophenol	1031:1067	anthropogenic pollutant p-nitrophenol to p-aminophenol	1031:1084	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	11	2	theme	magnetic	1491:1498	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	2	3	theme	fish	324:327	arg1	chitosan					314:321	chitosan	314:321	chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent	314:523	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	3	theme	fish	324:327	arg1	industry-waste					329:342	fish industry-waste	324:342	fish industry-waste	324:342	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	1	4	theme	nano-catalyst	246:258	arg1	nanocomposite					282:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	11	5	theme	Chitosan	1476:1483	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	1	6	theme	Fe3O4	261:265	arg1	nanocomposite					282:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	4	7	theme	EDX	626:628	arg1	spectra					630:636	EDX spectra	626:636	EDX spectra	626:636	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	9	8	theme	ICP-Ms	1135:1140	arg1	data					1142:1145	ICP-Ms data	1135:1145	ICP-Ms data for cumulative silver release	1135:1175	ICP-Ms data for cumulative silver release shows prepared nanocatalyst remains active and stable even after recycled for more than 15 times.					
31972199	8	9	contain	has	1090:1092	arg1	It					1087:1088	It	1087:1088	It	1087:1088	It has a very convenient magnetic separability.					
31972199	8	9	contain	has	1090:1092	arg2	separability					1121:1132	a very convenient magnetic separability	1094:1132	a very convenient magnetic separability	1094:1132	It has a very convenient magnetic separability.					
31972199	8	10	theme	magnetic	1112:1119	arg1	separability					1121:1132	a very convenient magnetic separability	1094:1132	a very convenient magnetic separability	1094:1132	It has a very convenient magnetic separability.					
31972199	5	11	theme	product	788:794	arg1	chitosan					800:807	the product was chitosan	784:807	the product was chitosan doped iron oxide with surfaced silver nanoparticles	784:859	XRD patterns revealed the product was chitosan doped iron oxide with surfaced silver nanoparticles.					
31972199	7	12	theme	effective	979:987	arg1	it					958:959	it	958:959	it	958:959	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	7	12	theme	effective	979:987	arg1	catalyst					1001:1008	an effective solid-phase catalyst	976:1008	an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol	976:1084	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	9	13	theme	silver	1162:1167	arg1	release					1169:1175	cumulative silver release	1151:1175	cumulative silver release	1151:1175	ICP-Ms data for cumulative silver release shows prepared nanocatalyst remains active and stable even after recycled for more than 15 times.					
31972199	3	14	theme	TEM	526:528	arg1	images					530:535	TEM images	526:535	TEM images	526:535	TEM images confirm the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm.					
31972199	1	15	theme	novel	187:191	arg1	approach					193:200	a novel approach	185:200	a novel approach	185:200	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	0	16	theme	anthropogenic	119:131	arg1	pathogens					160:168	anthropogenic pollutant and agricultural pathogens	119:168	anthropogenic pollutant and agricultural pathogens	119:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	5	17	theme	XRD	762:764	arg1	patterns					766:773	XRD patterns	762:773	XRD patterns	762:773	XRD patterns revealed the product was chitosan doped iron oxide with surfaced silver nanoparticles.					
31972199	3	18	theme	average	549:555	arg1	diameter					562:569	the average mean diameter	545:569	the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm	545:611	TEM images confirm the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm.					
31972199	4	19	theme	Ag	684:685	arg1	elements					701:708	Ag, Fe, C, and O elements	684:708	Ag, Fe, C, and O elements	684:708	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	0	20	theme	pathogens	160:168	arg1	reduction					106:114	catalytic reduction	96:114	catalytic reduction of anthropogenic pollutant and agricultural pathogens	96:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	5	21	theme	silver	840:845	arg1	nanoparticles					847:859	surfaced silver nanoparticles	831:859	surfaced silver nanoparticles	831:859	XRD patterns revealed the product was chitosan doped iron oxide with surfaced silver nanoparticles.					
31972199	4	22	theme	ICP-MS	643:648	arg1	data					650:653	ICP-MS data	643:653	ICP-MS data	643:653	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	0	23	theme	Fe3O4	56:60	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens	32:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	23	theme	Fe3O4	56:60	arg1	nanocomposite					77:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	3	24	theme	composed	574:581	arg1	nanocatalyst					583:594	composed nanocatalyst	574:594	composed nanocatalyst about 8 ± 2.2 nm	574:611	TEM images confirm the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm.					
31972199	2	25	theme	total	458:462	arg1	NaBH4					435:439	NaBH4	435:439	NaBH4	435:439	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	25	theme	total	458:462	arg1	solids					472:477	total soluble solids	458:477	total soluble solids (dairy effluent-waste)	458:500	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	25	theme	total	458:462	arg1	effluent-waste					486:499	dairy effluent-waste	480:499	dairy effluent-waste	480:499	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	10	26	theme	Aspergillus	1395:1405	arg1	niger					1407:1411	Aspergillus niger	1395:1411	Aspergillus niger	1395:1411	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	11	27	theme	@	1475:1475	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	0	28	theme	Chitosan	62:69	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens	32:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	28	theme	Chitosan	62:69	arg1	nanocomposite					77:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	5	29	theme	iron	815:818	arg1	oxide					820:824	iron oxide	815:824	iron oxide	815:824	XRD patterns revealed the product was chitosan doped iron oxide with surfaced silver nanoparticles.					
31972199	11	30	theme	nanostructures	1455:1468	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	6	31	theme	elemental	896:904	arg1	state					917:921	elemental electronic state	896:921	elemental electronic state	896:921	XPS and FTIR confirm composition, elemental electronic state, and binding energy.					
31972199	2	32	theme	dairy	480:484	arg1	solids					472:477	total soluble solids	458:477	total soluble solids (dairy effluent-waste)	458:500	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	32	theme	dairy	480:484	arg1	effluent-waste					486:499	dairy effluent-waste	480:499	dairy effluent-waste	480:499	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	10	33	theme	Pyricularia	1418:1428	arg1	sp					1430:1431	Pyricularia sp	1418:1431	Pyricularia sp	1418:1431	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	4	34	theme	elements	701:708	arg1	co-presence					669:679	the co-presence	665:679	the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag	665:750	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	4	35	theme	Fe	688:689	arg1	elements					701:708	Ag, Fe, C, and O elements	684:708	Ag, Fe, C, and O elements	684:708	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	0	36	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.	0:169	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	1	37	theme	@	266:266	arg1	nanocomposite					282:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	4	38	theme	C	692:692	arg1	elements					701:708	Ag, Fe, C, and O elements	684:708	Ag, Fe, C, and O elements	684:708	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	1	39	theme	-AgNP	276:280	arg1	nanocomposite					282:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	2	40	theme	active	379:384	arg1	nanoparticles					393:405	active silver nanoparticles	379:405	active silver nanoparticles on the exterior shell	379:427	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	4	41	theme	percentage	728:737	arg1	weight					739:744	a calculated percentage weight	715:744	a calculated percentage weight of Ag	715:750	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	10	42	theme	antifungal	1294:1303	arg1	activity					1305:1312	an excellent antifungal activity	1281:1312	an excellent antifungal activity	1281:1312	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	2	43	from	nanoparticles	393:405	arg1	shell					423:427	the exterior shell	410:427	the exterior shell	410:427	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	7	44	theme	anthropogenic	1031:1043	arg1	p-nitrophenol					1055:1067	anthropogenic pollutant p-nitrophenol	1031:1067	anthropogenic pollutant p-nitrophenol to p-aminophenol	1031:1084	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	1	45	theme	magnetic	237:244	arg1	nanocomposite					282:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	2	46	with	core	369:372	arg1	nanoparticles					393:405	active silver nanoparticles	379:405	active silver nanoparticles on the exterior shell	379:427	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	11	47	theme	-AgNP	1485:1489	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	8	48	theme	convenient	1101:1110	arg1	separability					1121:1132	a very convenient magnetic separability	1094:1132	a very convenient magnetic separability	1094:1132	It has a very convenient magnetic separability.					
31972199	11	49	dep	catalysis	1553:1561	arg1	applications					1579:1590	applications	1579:1590	applications	1579:1590	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	7	50	theme	solid-phase	989:999	arg1	it					958:959	it	958:959	it	958:959	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	7	50	theme	solid-phase	989:999	arg1	catalyst					1001:1008	an effective solid-phase catalyst	976:1008	an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol	976:1084	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	11	51	theme	greater	1532:1538	arg1	potential					1540:1548	greater potential	1532:1548	greater potential in catalysis and agriculture applications	1532:1590	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	7	52	used	utilized	964:971	arg2	catalyst					1001:1008	an effective solid-phase catalyst	976:1008	an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol	976:1084	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	7	52	used	utilized	964:971	arg2	it					958:959	it	958:959	it	958:959	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	10	53	theme	agricultural	1339:1350	arg1	pathogens					1352:1360	the agricultural pathogens	1335:1360	the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp	1335:1431	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	10	53	theme	agricultural	1339:1350	arg1	sp					1430:1431	Pyricularia sp	1418:1431	Pyricularia sp	1418:1431	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	10	53	theme	agricultural	1339:1350	arg1	coccodes					1385:1392	Colletotrichum coccodes	1370:1392	Colletotrichum coccodes	1370:1392	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	10	53	theme	agricultural	1339:1350	arg1	niger					1407:1411	Aspergillus niger	1395:1411	Aspergillus niger	1395:1411	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	11	54	from	potential	1540:1548	arg1	catalysis					1553:1561	catalysis	1553:1561	catalysis	1553:1561	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	11	54	from	potential	1540:1548	arg1	agriculture					1567:1577	agriculture	1567:1577	agriculture	1567:1577	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	2	55	theme	stabilizing	507:517	arg1	NaBH4					435:439	NaBH4	435:439	NaBH4	435:439	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	55	theme	stabilizing	507:517	arg1	agent					519:523	a stabilizing agent	505:523	a stabilizing agent	505:523	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	1	56	dep	synthesis	219:227	arg1	advance					229:235	advance	229:235	advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	229:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	0	57	theme	pollutant	133:141	arg1	pathogens					160:168	anthropogenic pollutant and agricultural pathogens	119:168	anthropogenic pollutant and agricultural pathogens	119:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	3	58	theme	mean	557:560	arg1	diameter					562:569	the average mean diameter	545:569	the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm	545:611	TEM images confirm the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm.					
31972199	4	59	theme	SEM	614:616	arg1	images					618:623	SEM images	614:623	SEM images	614:623	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	0	60	theme	agricultural	147:158	arg1	pathogens					160:168	anthropogenic pollutant and agricultural pathogens	119:168	anthropogenic pollutant and agricultural pathogens	119:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	9	61	theme	prepared	1183:1190	arg1	nanocatalyst					1192:1203	prepared nanocatalyst	1183:1203	prepared nanocatalyst	1183:1203	ICP-Ms data for cumulative silver release shows prepared nanocatalyst remains active and stable even after recycled for more than 15 times.					
31972199	9	62	theme	cumulative	1151:1160	arg1	release					1169:1175	cumulative silver release	1151:1175	cumulative silver release	1151:1175	ICP-Ms data for cumulative silver release shows prepared nanocatalyst remains active and stable even after recycled for more than 15 times.					
31972199	3	63	theme	nanocatalyst	583:594	arg1	diameter					562:569	the average mean diameter	545:569	the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm	545:611	TEM images confirm the average mean diameter of composed nanocatalyst about 8 ± 2.2 nm.					
31972199	5	64	theme	surfaced	831:838	arg1	nanoparticles					847:859	surfaced silver nanoparticles	831:859	surfaced silver nanoparticles	831:859	XRD patterns revealed the product was chitosan doped iron oxide with surfaced silver nanoparticles.					
31972199	0	65	theme	@	61:61	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens	32:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	65	theme	@	61:61	arg1	nanocomposite					77:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	5	66	theme	was	796:798	arg1	chitosan					800:807	the product was chitosan	784:807	the product was chitosan doped iron oxide with surfaced silver nanoparticles	784:859	XRD patterns revealed the product was chitosan doped iron oxide with surfaced silver nanoparticles.					
31972199	6	67	theme	binding	928:934	arg1	energy					936:941	binding energy	928:941	binding energy	928:941	XPS and FTIR confirm composition, elemental electronic state, and binding energy.					
31972199	2	68	theme	soluble	464:470	arg1	NaBH4					435:439	NaBH4	435:439	NaBH4	435:439	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	68	theme	soluble	464:470	arg1	solids					472:477	total soluble solids	458:477	total soluble solids (dairy effluent-waste)	458:500	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	68	theme	soluble	464:470	arg1	effluent-waste					486:499	dairy effluent-waste	480:499	dairy effluent-waste	480:499	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	11	69	theme	Fe3O4	1470:1474	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	0	70	theme	-AgNP	71:75	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens	32:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	70	theme	-AgNP	71:75	arg1	nanocomposite					77:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	71	from	fabrication	7:17	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens	32:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	71	from	fabrication	7:17	arg1	nanocomposite					77:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	11	72	theme	functionalized	1440:1453	arg1	nanocomposite					1500:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite	1440:1512	Thus, functionalized nanostructures Fe3O4@Chitosan -AgNP magnetic nanocomposite can probably hold greater potential in catalysis and agriculture applications.					
31972199	6	73	theme	electronic	906:915	arg1	state					917:921	elemental electronic state	896:921	elemental electronic state	896:921	XPS and FTIR confirm composition, elemental electronic state, and binding energy.					
31972199	10	74	theme	Colletotrichum	1370:1383	arg1	coccodes					1385:1392	Colletotrichum coccodes	1370:1392	Colletotrichum coccodes	1370:1392	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
31972199	0	75	theme	catalytic	96:104	arg1	reduction					106:114	catalytic reduction	96:114	catalytic reduction of anthropogenic pollutant and agricultural pathogens	96:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	4	76	theme	O	699:699	arg1	elements					701:708	Ag, Fe, C, and O elements	684:708	Ag, Fe, C, and O elements	684:708	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	2	77	theme	magnetic	360:367	arg1	core					369:372	the magnetic core	356:372	the magnetic core with active silver nanoparticles on the exterior shell	356:427	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	2	78	theme	exterior	414:421	arg1	shell					423:427	the exterior shell	410:427	the exterior shell	410:427	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	0	79	theme	silver	22:27	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.	0:169	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	1	80	theme	Chitosan	267:274	arg1	nanocomposite					282:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite	237:294	In this work, a novel approach was developed to synthesis advance magnetic nano-catalyst; Fe3O4@Chitosan -AgNP nanocomposite.					
31972199	2	81	theme	silver	386:391	arg1	nanoparticles					393:405	active silver nanoparticles	379:405	active silver nanoparticles on the exterior shell	379:427	It is made up of chitosan (fish industry-waste) doped onto the magnetic core with active silver nanoparticles on the exterior shell using NaBH4 as reductant and total soluble solids (dairy effluent-waste) as a stabilizing agent.					
31972199	0	82	theme	magnetic	32:39	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens	32:168	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	0	82	theme	magnetic	32:39	arg1	nanocomposite					77:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Fe3O4@Chitosan -AgNP nanocomposite	56:89	Facile fabrication of silver on magnetic nanocomposite (Fe3O4@Chitosan -AgNP nanocomposite) for catalytic reduction of anthropogenic pollutant and agricultural pathogens.					
31972199	7	83	theme	p-nitrophenol	1055:1067	arg1	reduction					1018:1026	the reduction	1014:1026	the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol	1014:1084	Additionally, it is utilized as an effective solid-phase catalyst for the reduction of anthropogenic pollutant p-nitrophenol to p-aminophenol.					
31972199	4	84	theme	Ag	749:750	arg1	weight					739:744	a calculated percentage weight	715:744	a calculated percentage weight of Ag	715:750	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	4	85	theme	calculated	717:726	arg1	weight					739:744	a calculated percentage weight	715:744	a calculated percentage weight of Ag	715:750	SEM images, EDX spectra, and ICP-MS data confirmed the co-presence of Ag, Fe, C, and O elements with a calculated percentage weight of Ag as 8.15%.					
31972199	10	86	theme	excellent	1284:1292	arg1	activity					1305:1312	an excellent antifungal activity	1281:1312	an excellent antifungal activity	1281:1312	Also, an excellent antifungal activity was observed against the agricultural pathogens such as Colletotrichum coccodes, Aspergillus niger, and Pyricularia sp.					
32155607	1	0	dep	poly	309:312	arg1	3HB-co-4HB					357:366	3HB-co-4HB	357:366	3HB-co-4HB	357:366	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	1	0	dep	poly	309:312	arg1	P					355:355	P	355:355	P(3HB-co-4HB)	355:367	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	8	1	theme	10:2	1483:1486	arg1	pullulan					1471:1478	pullulan	1471:1478	pullulan of 10:2	1471:1486	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	8	2	theme	/pullulan	1502:1510	arg1	nanofibers					1522:1531	P(3HB-co-4HB)/pullulan composite nanofibers	1489:1531	P(3HB-co-4HB)/pullulan composite nanofibers	1489:1531	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	3	3	theme	shell-core	698:707	arg1	structure					709:717	a shell-core structure	696:717	a shell-core structure	696:717	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	4	4	theme	P	882:882	arg1	nanofibers					924:933	P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers	882:933	P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers	882:933	Finally, P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers were prepared.					
32155607	7	5	theme	pullulan	1307:1314	arg1	content					1316:1322	the pullulan content	1303:1322	the pullulan content	1303:1322	As the pullulan content increases, the strain and stress of P(3HB-co-4HB)/pullulan nanofibers increase initially and decrease later.					
32155607	3	6	theme	core	861:864	arg1	layer					866:870	the core layer	857:870	the core layer	857:870	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	5	7	theme	crystallization	994:1008	arg1	properties					1010:1019	crystallization properties	994:1019	crystallization properties	994:1019	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	0	8	theme	shell-core	126:135	arg1	structure					137:145	shell-core structure	126:145	shell-core structure	126:145	Preparation and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/pullulan-gelatin electrospun nanofibers with shell-core structure.					
32155607	8	9	theme	uniform	1543:1549	arg1	morphology					1551:1560	a uniform morphology	1541:1560	a uniform morphology with an average diameter of 590 nm and porosity of 70.71%	1541:1618	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	5	10	theme	intermolecular	954:967	arg1	interaction					969:979	The intermolecular interaction	950:979	The intermolecular interaction	950:979	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	9	11	theme	potential	1761:1769	arg1	biomaterials					1787:1798	biomaterials	1787:1798	biomaterials without cytotoxicity	1787:1819	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	9	11	theme	potential	1761:1769	arg1	applications					1771:1782	potential applications	1761:1782	potential applications	1761:1782	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	1	12	theme	/pullulan	369:377	arg1	nanofibers					379:388	poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers	309:388	poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers	309:388	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	7	13	dep	strain	1339:1344	arg1	the					1335:1337	the	1335:1337	the	1335:1337	As the pullulan content increases, the strain and stress of P(3HB-co-4HB)/pullulan nanofibers increase initially and decrease later.					
32155607	3	14	with	nanofibers	680:689	arg1	structure					709:717	a shell-core structure	696:717	a shell-core structure	696:717	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	7	15	theme	/pullulan	1373:1381	arg1	stress					1350:1355	stress	1350:1355	stress	1350:1355	As the pullulan content increases, the strain and stress of P(3HB-co-4HB)/pullulan nanofibers increase initially and decrease later.					
32155607	7	15	theme	/pullulan	1373:1381	arg1	strain					1339:1344	strain	1339:1344	strain	1339:1344	As the pullulan content increases, the strain and stress of P(3HB-co-4HB)/pullulan nanofibers increase initially and decrease later.					
32155607	1	16	theme	nanofibers	379:388	arg1	preparation					294:304	the preparation	290:304	the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers	290:388	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	2	17	theme	composite	631:639	arg1	solution					641:648	the P(3HB-co-4HB)/pullulan composite solution	604:648	the P(3HB-co-4HB)/pullulan composite solution	604:648	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	5	18	theme	mechanical	1022:1031	arg1	properties					1033:1042	mechanical properties	1022:1042	mechanical properties	1022:1042	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	2	19	theme	/pullulan	621:629	arg1	solution					641:648	the P(3HB-co-4HB)/pullulan composite solution	604:648	the P(3HB-co-4HB)/pullulan composite solution	604:648	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	1	20	theme	drug	476:479	arg1	loading					481:487	the drug loading	472:487	the drug loading of composite fibers	472:507	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	6	21	theme	composite	1214:1222	arg1	nanofibers					1224:1233	P(3HB-co-4HB)/pullulan composite nanofibers	1191:1233	P(3HB-co-4HB)/pullulan composite nanofibers	1191:1233	Results show that the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers increases continuously with an increase in the pullulan content.					
32155607	9	22	theme	release	1721:1727	arg1	effect					1729:1734	a release effect	1719:1734	a release effect	1719:1734	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	1	23	theme	cell	208:211	arg1	adhesion					213:220	cell adhesion	208:220	cell adhesion	208:220	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	8	24	theme	average	1570:1576	arg1	diameter					1578:1585	an average diameter	1567:1585	an average diameter of 590 nm	1567:1595	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	morphology					1045:1054	morphology	1045:1054	morphology	1045:1054	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	biocompatibility					1080:1095	biocompatibility	1080:1095	biocompatibility	1080:1095	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	properties					1010:1019	crystallization properties	994:1019	crystallization properties	994:1019	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	release					1067:1073	sustained release	1057:1073	sustained release	1057:1073	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	interaction					969:979	The intermolecular interaction	950:979	The intermolecular interaction	950:979	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	morphology					982:991	morphology	982:991	morphology	982:991	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	5	25	theme	nanofibers	1110:1119	arg1	properties					1033:1042	mechanical properties	1022:1042	mechanical properties	1022:1042	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	6	26	theme	/pullulan	1204:1212	arg1	nanofibers					1224:1233	P(3HB-co-4HB)/pullulan composite nanofibers	1191:1233	P(3HB-co-4HB)/pullulan composite nanofibers	1191:1233	Results show that the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers increases continuously with an increase in the pullulan content.					
32155607	9	27	theme	/pullulan-gelatin/drug	1658:1679	arg1	structure					1692:1700	the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure	1641:1700	the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure	1641:1700	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	3	28	from	solution	845:852	arg1	layer					866:870	the core layer	857:870	the core layer	857:870	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	9	29	contain	has	1757:1759	arg2	applications					1771:1782	potential applications	1761:1782	potential applications	1761:1782	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	9	29	contain	has	1757:1759	arg1	structure					1692:1700	the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure	1641:1700	the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure	1641:1700	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	9	29	contain	has	1757:1759	arg2	biomaterials					1787:1798	biomaterials	1787:1798	biomaterials without cytotoxicity	1787:1819	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	7	30	theme	P	1360:1360	arg1	/pullulan					1373:1381	P(3HB-co-4HB)/pullulan	1360:1381	P(3HB-co-4HB)/pullulan	1360:1381	As the pullulan content increases, the strain and stress of P(3HB-co-4HB)/pullulan nanofibers increase initially and decrease later.					
32155607	7	31	theme	3HB-co-4HB	1362:1371	arg1	/pullulan					1373:1381	P(3HB-co-4HB)/pullulan	1360:1381	P(3HB-co-4HB)/pullulan	1360:1381	As the pullulan content increases, the strain and stress of P(3HB-co-4HB)/pullulan nanofibers increase initially and decrease later.					
32155607	8	32	with	morphology	1551:1560	arg1	diameter					1578:1585	an average diameter	1567:1585	an average diameter of 590 nm	1567:1595	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	8	32	with	morphology	1551:1560	arg1	porosity					1601:1608	porosity	1601:1608	porosity of 70.71%	1601:1618	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	1	33	theme	poly	309:312	arg1	nanofibers					379:388	poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers	309:388	poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers	309:388	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	2	34	theme	emulsifying	546:556	arg1	agent					558:562	the emulsifying agent	542:562	the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution	542:648	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	2	34	theme	emulsifying	546:556	arg1	polyglycoside					516:528	Alkyl polyglycoside	510:528	Alkyl polyglycoside	510:528	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	9	35	theme	shell-core	1681:1690	arg1	structure					1692:1700	the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure	1641:1700	the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure	1641:1700	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	5	36	theme	sustained	1057:1065	arg1	release					1067:1073	sustained release	1057:1073	sustained release	1057:1073	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	8	37	theme	nm	1594:1595	arg1	diameter					1578:1585	an average diameter	1567:1585	an average diameter of 590 nm	1567:1595	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	8	37	theme	nm	1594:1595	arg1	porosity					1601:1608	porosity	1601:1608	porosity of 70.71%	1601:1618	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	2	38	used	used	534:537	arg2	polyglycoside					516:528	Alkyl polyglycoside	510:528	Alkyl polyglycoside	510:528	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	2	38	used	used	534:537	arg2	agent					558:562	the emulsifying agent	542:562	the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution	542:648	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	8	39	theme	mass	1440:1443	arg1	ratio					1445:1449	the mass ratio	1436:1449	the mass ratio of P(3HB-co-4HB) to pullulan of 10:2	1436:1486	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	1	40	theme	composite	492:500	arg1	fibers					502:507	composite fibers	492:507	composite fibers	492:507	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	0	41	theme	electrospun	98:108	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/pullulan-gelatin electrospun nanofibers with shell-core structure.					
32155607	0	41	theme	electrospun	98:108	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/pullulan-gelatin electrospun nanofibers with shell-core structure.					
32155607	4	42	theme	shell-core	913:922	arg1	nanofibers					924:933	P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers	882:933	P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers	882:933	Finally, P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers were prepared.					
32155607	3	43	theme	Drug-loading	651:662	arg1	property					664:671	Drug-loading property	651:671	Drug-loading property of the nanofibers with a shell-core structure	651:717	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	8	44	theme	P	1454:1454	arg1	ratio					1445:1449	the mass ratio	1436:1449	the mass ratio of P(3HB-co-4HB) to pullulan of 10:2	1436:1486	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	1	45	theme	fibers	502:507	arg1	loading					481:487	the drug loading	472:487	the drug loading of composite fibers	472:507	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	4	46	theme	/pullulan-gelatin	895:911	arg1	nanofibers					924:933	P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers	882:933	P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers	882:933	Finally, P(3HB-co-4HB)/pullulan-gelatin shell-core nanofibers were prepared.					
32155607	6	47	theme	pullulan	1282:1289	arg1	content					1291:1297	the pullulan content	1278:1297	the pullulan content	1278:1297	Results show that the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers increases continuously with an increase in the pullulan content.					
32155607	3	48	theme	drug-containing	821:835	arg1	solution					845:852	a stable drug-containing gelatin solution	812:852	a stable drug-containing gelatin solution in the core layer	812:870	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	6	49	theme	nanofibers	1224:1233	arg1	property					1179:1186	the crystallization property	1159:1186	the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers	1159:1233	Results show that the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers increases continuously with an increase in the pullulan content.					
32155607	1	50	theme	P	445:445	arg1	biocompatibility					425:440	the biocompatibility	421:440	the biocompatibility of P(3HB-co-4HB)	421:457	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	1	51	theme	hydrophilic	163:173	arg1	modifier					277:284	a modifier	275:284	a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers	275:388	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	1	51	theme	hydrophilic	163:173	arg1	pullulan					175:182	hydrophilic pullulan	163:182	hydrophilic pullulan	163:182	In this study, hydrophilic pullulan, which is favorable for cell adhesion, proliferation, and differentiation, was selected as a modifier for the preparation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB))/pullulan nanofibers via electrospinning to improve the biocompatibility of P(3HB-co-4HB) and increase the drug loading of composite fibers.					
32155607	5	52	theme	composite	1100:1108	arg1	nanofibers					1110:1119	composite nanofibers	1100:1119	composite nanofibers	1100:1119	The intermolecular interaction, morphology, crystallization properties, mechanical properties, morphology, sustained release, and biocompatibility of composite nanofibers were characterized.					
32155607	3	53	theme	gelatin	837:843	arg1	solution					845:852	a stable drug-containing gelatin solution	812:852	a stable drug-containing gelatin solution in the core layer	812:870	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	2	54	theme	Alkyl	510:514	arg1	agent					558:562	the emulsifying agent	542:562	the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution	542:648	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	2	54	theme	Alkyl	510:514	arg1	polyglycoside					516:528	Alkyl polyglycoside	510:528	Alkyl polyglycoside	510:528	Alkyl polyglycoside was used as the emulsifying agent to promote emulsification and stabilize the P(3HB-co-4HB)/pullulan composite solution.					
32155607	3	55	theme	nanofibers	680:689	arg1	property					664:671	Drug-loading property	651:671	Drug-loading property of the nanofibers with a shell-core structure	651:717	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32155607	9	56	theme	mass	1629:1632	arg1	ratio					1634:1638	this mass ratio	1624:1638	this mass ratio	1624:1638	At this mass ratio, the P(3HB-co-4HB)/pullulan-gelatin/drug shell-core structure, which sustained a release effect for more than 180 h, has potential applications as biomaterials without cytotoxicity.					
32155607	6	57	theme	crystallization	1163:1177	arg1	property					1179:1186	the crystallization property	1159:1186	the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers	1159:1233	Results show that the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers increases continuously with an increase in the pullulan content.					
32155607	0	58	theme	/pullulan-gelatin	80:96	arg1	electrospun					98:108	poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/pullulan-gelatin electrospun	36:108	poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/pullulan-gelatin electrospun	36:108	Preparation and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/pullulan-gelatin electrospun nanofibers with shell-core structure.					
32155607	8	59	theme	composite	1512:1520	arg1	nanofibers					1522:1531	P(3HB-co-4HB)/pullulan composite nanofibers	1489:1531	P(3HB-co-4HB)/pullulan composite nanofibers	1489:1531	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	8	60	theme	%	1618:1618	arg1	diameter					1578:1585	an average diameter	1567:1585	an average diameter of 590 nm	1567:1595	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	8	60	theme	%	1618:1618	arg1	porosity					1601:1608	porosity	1601:1608	porosity of 70.71%	1601:1618	At the mass ratio of P(3HB-co-4HB) to pullulan of 10:2, P(3HB-co-4HB)/pullulan composite nanofibers exhibit a uniform morphology with an average diameter of 590 nm and porosity of 70.71%.					
32155607	6	61	from	increase	1266:1273	arg1	content					1291:1297	the pullulan content	1278:1297	the pullulan content	1278:1297	Results show that the crystallization property of P(3HB-co-4HB)/pullulan composite nanofibers increases continuously with an increase in the pullulan content.					
32155607	3	62	theme	stable	814:819	arg1	solution					845:852	a stable drug-containing gelatin solution	812:852	a stable drug-containing gelatin solution in the core layer	812:870	Drug-loading property of the nanofibers with a shell-core structure is increased because gelatin was not formed into fibers via electrospinning, thereby forming a stable drug-containing gelatin solution in the core layer.					
32863061	4	0	theme	urine	504:508	arg1	fingerprinting					454:467	METHODS Metabolic fingerprinting	436:467	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine	436:508	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	5	1	from	differences	661:671	arg1	pathways					724:731	the galactose and starch and sucrose metabolism pathways	676:731	the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	676:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	1	from	differences	661:671	arg1	urine					848:852	urine	848:852	urine	848:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	8	2	theme	observed	1173:1180	arg1	changes					1182:1188	the observed changes	1169:1188	the observed changes generating new hypothesis	1169:1214	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	3	theme	synthesis	800:808	arg1	alterations					765:775	alterations	765:775	alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine	765:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	3	theme	synthesis	800:808	arg1	differences					661:671	Significant differences	649:671	Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	649:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	7	4	theme	DHM	1036:1038	arg1	feeding					1025:1031	feeding	1025:1031	feeding of DHM	1025:1038	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	2	5	theme	pasteurized	265:275	arg1	DHM					295:297	DHM	295:297	DHM	295:297	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	2	5	theme	pasteurized	265:275	arg1	milk					289:292	pasteurized donor human milk	265:292	pasteurized donor human milk (DHM)	265:298	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	5	6	theme	pyrimidine	814:823	arg1	pathways					836:843	pyrimidine metabolism pathways	814:843	pyrimidine metabolism pathways	814:843	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	0	7	from	interactions	64:75	arg1	infants					88:94	preterm infants	80:94	preterm infants	80:94	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	8	8	attach	present	1237:1243	arg1	HM					1248:1249	HM	1248:1249	HM	1248:1249	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	8	attach	present	1237:1243	arg2	hormones					1228:1235	steroid hormones	1220:1235	steroid hormones	1220:1235	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	9	theme	steroid	784:790	arg1	synthesis					800:808	steroid hormone synthesis	784:808	steroid hormone synthesis	784:808	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	8	10	theme	pyrimidine	1368:1377	arg1	metabolism					1379:1388	the pyrimidine metabolism	1364:1388	the pyrimidine metabolism	1364:1388	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	11	theme	steroid	1220:1226	arg1	hormones					1228:1235	steroid hormones	1220:1235	steroid hormones	1220:1235	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	12	from	alterations	765:775	arg1	pathways					724:731	the galactose and starch and sucrose metabolism pathways	676:731	the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	676:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	12	from	alterations	765:775	arg1	urine					848:852	urine	848:852	urine	848:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	1	13	theme	AIMS	110:113	arg1	milk					121:124	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	1	13	theme	AIMS	110:113	arg1	gold					133:136	the gold	129:136	the gold standard for infant nutrition	129:166	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	4	14	theme	16S	620:622	arg1	sequencing					629:638	16S rRNA sequencing	620:638	16S rRNA sequencing	620:638	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	7	15	contain	has	1040:1042	arg2	impact					1058:1063	a significant impact	1044:1063	a significant impact on the metabolic phenotype and microbiota of preterm infants	1044:1124	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	7	15	contain	has	1040:1042	arg1	feeding					1025:1031	feeding	1025:1031	feeding of DHM	1025:1038	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	0	16	theme	donor	10:14	arg1	milk					22:25	donor human milk	10:25	donor human milk	10:25	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	5	17	theme	pathways	836:843	arg1	alterations					765:775	alterations	765:775	alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine	765:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	17	theme	pathways	836:843	arg1	differences					661:671	Significant differences	649:671	Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	649:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	18	theme	metabolism	713:722	arg1	pathways					724:731	the galactose and starch and sucrose metabolism pathways	676:731	the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	676:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	1	19	theme	standard	138:145	arg1	milk					121:124	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	1	19	theme	standard	138:145	arg1	gold					133:136	the gold	129:136	the gold standard for infant nutrition	129:166	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	0	20	from	Effect	0:5	arg1	interactions					64:75	metabolic interactions	54:75	metabolic interactions	54:75	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	0	20	from	Effect	0:5	arg1	microbiota					39:48	host-gut microbiota	30:48	host-gut microbiota	30:48	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	1	21	theme	BACKGROUND	97:106	arg1	AIMS					110:113	BACKGROUND & AIMS	97:113	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	0	22	theme	preterm	80:86	arg1	infants					88:94	preterm infants	80:94	preterm infants	80:94	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	9	23	theme	long-term	1468:1476	arg1	implications					1478:1489	Short- and long-term implications	1457:1489	Short- and long-term implications of the observed changes for preterm infants	1457:1533	Short- and long-term implications of the observed changes for preterm infants need to be assessed in further studies.					
32863061	6	24	theme	gut-microbiota	921:934	arg1	composition					936:946	the gut-microbiota composition	917:946	the gut-microbiota composition	917:946	Differences in the gut-microbiota composition were also identified.					
32863061	0	25	theme	metabolic	54:62	arg1	interactions					64:75	metabolic interactions	54:75	metabolic interactions	54:75	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	9	26	theme	observed	1498:1505	arg1	changes					1507:1513	the observed changes	1494:1513	the observed changes	1494:1513	Short- and long-term implications of the observed changes for preterm infants need to be assessed in further studies.					
32863061	3	27	theme	metabolic	312:320	arg1	signatures					322:331	metabolic signatures	312:331	metabolic signatures of OMM and DHM	312:346	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	6	28	from	Differences	902:912	arg1	composition					936:946	the gut-microbiota composition	917:946	the gut-microbiota composition	917:946	Differences in the gut-microbiota composition were also identified.					
32863061	8	29	theme	steroid	1303:1309	arg1	pathway					1332:1338	the steroid hormone biosynthesis pathway	1299:1338	the steroid hormone biosynthesis pathway	1299:1338	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	3	30	theme	developing	389:398	arg1	microbiota					400:409	the developing microbiota	385:409	the developing microbiota	385:409	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	7	31	theme	preterm	1110:1116	arg1	infants					1118:1124	preterm infants	1110:1124	preterm infants	1110:1124	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	5	32	theme	feeding	893:899	arg1	type					885:888	the type	881:888	the type of feeding	881:899	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	8	33	theme	biosynthesis	1319:1330	arg1	pathway					1332:1338	the steroid hormone biosynthesis pathway	1299:1338	the steroid hormone biosynthesis pathway	1299:1338	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	0	34	theme	milk	22:25	arg1	Effect					0:5	Effect	0:5	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.	0:95	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	7	35	from	impact	1058:1063	arg1	microbiota					1096:1105	microbiota	1096:1105	microbiota	1096:1105	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	7	35	from	impact	1058:1063	arg1	phenotype					1082:1090	metabolic phenotype	1072:1090	metabolic phenotype	1072:1090	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	0	36	theme	host-gut	30:37	arg1	microbiota					39:48	host-gut microbiota	30:48	host-gut microbiota	30:48	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	2	37	theme	sufficient	221:230	arg1	OMM					251:253	OMM	251:253	OMM	251:253	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	2	37	theme	sufficient	221:230	arg1	milk					245:248	mother's milk	236:248	sufficient own mother's milk (OMM)	221:254	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	2	38	theme	Preterm	169:175	arg1	infants					177:183	Preterm infants	169:183	Preterm infants	169:183	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	1	39	theme	infant	151:156	arg1	nutrition					158:166	infant nutrition	151:166	infant nutrition	151:166	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	9	40	theme	further	1558:1564	arg1	studies					1566:1572	further studies	1558:1572	further studies	1558:1572	Short- and long-term implications of the observed changes for preterm infants need to be assessed in further studies.					
32863061	4	41	theme	chromatography-mass	537:555	arg1	spectrometry					557:568	liquid chromatography-mass spectrometry	530:568	liquid chromatography-mass spectrometry	530:568	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	7	42	theme	significant	1046:1056	arg1	impact					1058:1063	a significant impact	1044:1063	a significant impact on the metabolic phenotype and microbiota of preterm infants	1044:1124	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	8	43	from	activity	1287:1294	arg1	infants					1351:1357	preterm infants	1343:1357	preterm infants	1343:1357	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	44	theme	hormone	792:798	arg1	synthesis					800:808	steroid hormone synthesis	784:808	steroid hormone synthesis	784:808	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	4	45	theme	stool	587:591	arg1	microbiota					593:602	the infant's stool microbiota	574:602	the infant's stool microbiota	574:602	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	2	46	theme	donor	277:281	arg1	DHM					295:297	DHM	295:297	DHM	295:297	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	2	46	theme	donor	277:281	arg1	milk					289:292	pasteurized donor human milk	265:292	pasteurized donor human milk (DHM)	265:298	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	8	47	theme	changes	1182:1188	arg1	origin					1159:1164	the origin	1155:1164	the origin of the observed changes generating new hypothesis	1155:1214	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	48	theme	pathway	1332:1338	arg1	activity					1287:1294	the activity	1283:1294	the activity of the steroid hormone biosynthesis pathway in preterm infants	1283:1357	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	9	49	theme	Short-	1457:1462	arg1	implications					1478:1489	Short- and long-term implications	1457:1489	Short- and long-term implications of the observed changes for preterm infants	1457:1533	Short- and long-term implications of the observed changes for preterm infants need to be assessed in further studies.					
32863061	8	50	theme	new	1201:1203	arg1	hypothesis					1205:1214	new hypothesis	1201:1214	new hypothesis	1201:1214	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	51	theme	Significant	649:659	arg1	differences					661:671	Significant differences	649:671	Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	649:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	7	52	theme	DHM	1000:1002	arg1	composition					985:995	The composition	981:995	The composition of DHM	981:1002	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	1	53	theme	&	108:108	arg1	AIMS					110:113	BACKGROUND & AIMS	97:113	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	4	54	theme	Metabolic	444:452	arg1	fingerprinting					454:467	METHODS Metabolic fingerprinting	436:467	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine	436:508	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	1	55	theme	Human	115:119	arg1	milk					121:124	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk	97:124	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	1	55	theme	Human	115:119	arg1	gold					133:136	the gold	129:136	the gold standard for infant nutrition	129:166	BACKGROUND & AIMS Human milk is the gold standard for infant nutrition.					
32863061	8	56	theme	preterm	1406:1412	arg1	infants					1414:1420	preterm infants	1406:1420	preterm infants	1406:1420	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	57	theme	starch	694:699	arg1	metabolism					713:722	starch and sucrose metabolism	694:722	metabolism	713:722	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	2	58	theme	own	232:234	arg1	OMM					251:253	OMM	251:253	OMM	251:253	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	2	58	theme	own	232:234	arg1	milk					245:248	mother's milk	236:248	sufficient own mother's milk (OMM)	221:254	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	5	59	theme	metabolism	825:834	arg1	pathways					836:843	pyrimidine metabolism pathways	814:843	pyrimidine metabolism pathways	814:843	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	4	60	theme	METHODS	436:442	arg1	fingerprinting					454:467	METHODS Metabolic fingerprinting	436:467	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine	436:508	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	5	61	theme	sucrose	705:711	arg1	metabolism					713:722	starch and sucrose metabolism	694:722	metabolism	713:722	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	62	from	synthesis	800:808	arg1	urine					848:852	urine	848:852	urine	848:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	0	63	from	microbiota	39:48	arg1	infants					88:94	preterm infants	80:94	preterm infants	80:94	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	5	64	dep	RESULTS	641:647	arg1	observed					859:866	observed	859:866	were observed depending on the type of feeding	854:899	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	4	65	theme	rRNA	624:627	arg1	sequencing					629:638	16S rRNA sequencing	620:638	16S rRNA sequencing	620:638	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	5	66	from	urine	848:852	arg1	alterations					765:775	alterations	765:775	alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine	765:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	5	66	from	urine	848:852	arg1	differences					661:671	Significant differences	649:671	Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	649:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	8	67	theme	gut-microbiota	1441:1454	arg1	activity					1429:1436	the activity	1425:1436	the activity of gut-microbiota	1425:1454	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	68	theme	significant	1258:1268	arg1	influence					1270:1278	a significant influence	1256:1278	a significant influence	1256:1278	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	69	theme	galactose	680:688	arg1	pathways					724:731	the galactose and starch and sucrose metabolism pathways	676:731	the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM	676:758	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	3	70	from	signatures	322:331	arg1	interplay					372:380	the interplay	368:380	the interplay of the developing microbiota	368:409	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	3	70	from	signatures	322:331	arg1	metabolism					424:433	infant's metabolism	415:433	infant's metabolism	415:433	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	5	71	from	pathways	836:843	arg1	urine					848:852	urine	848:852	urine	848:852	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	7	72	dep	phenotype	1082:1090	arg1	the					1068:1070	the	1068:1070	the	1068:1070	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	7	73	dep	CONCLUSION	970:979	arg1	has					1040:1042	has	1040:1042	has a significant impact on the metabolic phenotype and microbiota of preterm infants	1040:1124	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	7	73	dep	CONCLUSION	970:979	arg1	differs					1004:1010	differs	1004:1010	differs from OMM	1004:1019	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	7	74	theme	infants	1118:1124	arg1	microbiota					1096:1105	microbiota	1096:1105	microbiota	1096:1105	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	7	74	theme	infants	1118:1124	arg1	phenotype					1082:1090	metabolic phenotype	1072:1090	metabolic phenotype	1072:1090	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	3	75	theme	OMM	336:338	arg1	effect					358:363	their effect	352:363	their effect on the interplay of the developing microbiota and infant's metabolism	352:433	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	3	75	theme	OMM	336:338	arg1	signatures					322:331	metabolic signatures	312:331	metabolic signatures of OMM and DHM	312:346	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	0	76	theme	human	16:20	arg1	milk					22:25	donor human milk	10:25	donor human milk	10:25	Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants.					
32863061	9	77	theme	changes	1507:1513	arg1	implications					1478:1489	Short- and long-term implications	1457:1489	Short- and long-term implications of the observed changes for preterm infants	1457:1533	Short- and long-term implications of the observed changes for preterm infants need to be assessed in further studies.					
32863061	3	78	theme	microbiota	400:409	arg1	interplay					372:380	the interplay	368:380	the interplay of the developing microbiota	368:409	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	3	78	theme	microbiota	400:409	arg1	metabolism					424:433	infant's metabolism	415:433	infant's metabolism	415:433	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	9	79	theme	preterm	1519:1525	arg1	infants					1527:1533	preterm infants	1519:1533	preterm infants	1519:1533	Short- and long-term implications of the observed changes for preterm infants need to be assessed in further studies.					
32863061	4	80	theme	OMM	472:474	arg1	fingerprinting					454:467	METHODS Metabolic fingerprinting	436:467	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine	436:508	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	8	81	theme	hormone	1311:1317	arg1	pathway					1332:1338	the steroid hormone biosynthesis pathway	1299:1338	the steroid hormone biosynthesis pathway	1299:1338	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	7	82	theme	metabolic	1072:1080	arg1	phenotype					1082:1090	metabolic phenotype	1072:1090	metabolic phenotype	1072:1090	CONCLUSION The composition of DHM differs from OMM and feeding of DHM has a significant impact on the metabolic phenotype and microbiota of preterm infants.					
32863061	3	83	theme	DHM	344:346	arg1	effect					358:363	their effect	352:363	their effect on the interplay of the developing microbiota and infant's metabolism	352:433	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	3	83	theme	DHM	344:346	arg1	signatures					322:331	metabolic signatures	312:331	metabolic signatures of OMM and DHM	312:346	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	4	84	theme	DHM	480:482	arg1	fingerprinting					454:467	METHODS Metabolic fingerprinting	436:467	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine	436:508	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	8	85	dep	help	1136:1139	arg1	ii					1360:1361	ii	1360:1361	ii	1360:1361	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	85	dep	help	1136:1139	arg1	modulated					1393:1401	modulated	1393:1401	is modulated in preterm infants by the activity of gut-microbiota	1390:1454	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	85	dep	help	1136:1139	arg1	present					1237:1243	present	1237:1243	present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants	1237:1357	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	8	85	dep	help	1136:1139	arg1	i					1217:1217	i	1217:1217	i	1217:1217	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	5	86	dep	synthesis	800:808	arg1	the					780:782	the	780:782	the	780:782	RESULTS Significant differences in the galactose and starch and sucrose metabolism pathways when comparing OMM and DHM, and alterations of the steroid hormone synthesis and pyrimidine metabolism pathways in urine were observed depending on the type of feeding.					
32863061	8	87	dep	present	1237:1243	arg1	have					1251:1254	have	1251:1254	present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants	1237:1357	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	4	88	theme	liquid	530:535	arg1	spectrometry					557:568	liquid chromatography-mass spectrometry	530:568	liquid chromatography-mass spectrometry	530:568	METHODS Metabolic fingerprinting of OMM and DHM as well as infant's urine was performed using liquid chromatography-mass spectrometry and the infant's stool microbiota was analyzed by 16S rRNA sequencing.					
32863061	8	89	theme	preterm	1343:1349	arg1	infants					1351:1357	preterm infants	1343:1357	preterm infants	1343:1357	Our data help to understand the origin of the observed changes generating new hypothesis: i) steroid hormones present in HM have a significant influence in the activity of the steroid hormone biosynthesis pathway in preterm infants; ii) the pyrimidine metabolism is modulated in preterm infants by the activity of gut-microbiota.					
32863061	3	90	from	effect	358:363	arg1	interplay					372:380	the interplay	368:380	the interplay of the developing microbiota	368:409	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	3	90	from	effect	358:363	arg1	metabolism					424:433	infant's metabolism	415:433	infant's metabolism	415:433	We studied metabolic signatures of OMM and DHM and their effect on the interplay of the developing microbiota and infant's metabolism.					
32863061	2	91	theme	human	283:287	arg1	DHM					295:297	DHM	295:297	DHM	295:297	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
32863061	2	91	theme	human	283:287	arg1	milk					289:292	pasteurized donor human milk	265:292	pasteurized donor human milk (DHM)	265:298	Preterm infants whose mothers are unable to provide sufficient own mother's milk (OMM), receive pasteurized donor human milk (DHM).					
33572312	0	0	theme	Fuel	105:108	arg1	Cells					110:114	Direct Alkaline Ethanol Fuel Cells	81:114	Direct Alkaline Ethanol Fuel Cells	81:114	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	3	1	theme	graphene	664:671	arg1	derivatives					673:683	N-doped graphene derivatives	656:683	N-doped graphene derivatives	656:683	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	1	2	theme	membranes	163:171	arg1	series					139:144	a series	137:144	a series of nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives	137:276	Herein, we prepared a series of nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives.					
33572312	1	3	theme	graphene	257:264	arg1	derivatives					266:276	three compositionally and structurally different N-doped graphene derivatives	200:276	three compositionally and structurally different N-doped graphene derivatives	200:276	Herein, we prepared a series of nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives.					
33572312	0	4	theme	Ethanol	97:103	arg1	Fuel					105:108	Direct Alkaline Ethanol Fuel	81:108	Direct Alkaline Ethanol Fuel Cells	81:114	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	3	5	dep	largest	629:635	arg1	%					642:642	0.07%	638:642	0.07%	638:642	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	4	6	theme	alkaline	886:893	arg1	test					903:906	direct alkaline ethanol test	879:906	direct alkaline ethanol test cells	879:912	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	3	7	theme	tensile	771:777	arg1	properties					779:788	tensile properties	771:788	tensile properties	771:788	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	4	8	theme	direct	879:884	arg1	test					903:906	direct alkaline ethanol test	879:906	direct alkaline ethanol test cells	879:912	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	3	9	theme	derivatives	673:683	arg1	loading					645:651	The largest (0.07%) loading	625:651	The largest (0.07%) loading of N-doped graphene derivatives	625:683	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	2	10	theme	group	609:613	arg1	contents					615:622	group contents	609:622	group contents	609:622	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	11	dep	morphology	533:542	arg1	contents					615:622	group contents	609:622	group contents	609:622	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	6	12	theme	fuel	1149:1152	arg1	cells					1154:1158	direct ethanol fuel cells	1134:1158	direct ethanol fuel cells	1134:1158	= 2.2 mWcm-2), suggesting the potential of the newly proposed membranes for application in direct ethanol fuel cells.					
33572312	4	13	theme	ethanol	895:901	arg1	test					903:906	direct alkaline ethanol test	879:906	direct alkaline ethanol test cells	879:912	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	3	14	theme	N-doped	656:662	arg1	derivatives					673:683	N-doped graphene derivatives	656:683	N-doped graphene derivatives	656:683	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	6	15	theme	ethanol	1141:1147	arg1	cells					1154:1158	direct ethanol fuel cells	1134:1158	direct ethanol fuel cells	1134:1158	= 2.2 mWcm-2), suggesting the potential of the newly proposed membranes for application in direct ethanol fuel cells.					
33572312	4	16	located	found	922:926	arg2	it					915:916	it	915:916	it	915:916	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	4	16	located	found	922:926	arg1	cells					908:912	direct alkaline ethanol test cells	879:912	direct alkaline ethanol test cells	879:912	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	0	17	theme	Efficient	0:8	arg1	Composite					57:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	4	18	theme	test	903:906	arg1	cells					908:912	direct alkaline ethanol test cells	879:912	direct alkaline ethanol test cells	879:912	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	0	19	theme	Chitosan/Nitrogen-Doped	10:32	arg1	Composite					57:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	2	20	dep	nitrogen	549:556	arg1	pyrrolic					570:577	pyrrolic	570:577	pyrrolic	570:577	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	20	dep	nitrogen	549:556	arg1	quaternary					584:593	quaternary	584:593	quaternary	584:593	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	20	dep	nitrogen	549:556	arg1	pyridinic					559:567	pyridinic	559:567	pyridinic	559:567	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	6	21	theme	direct	1134:1139	arg1	cells					1154:1158	direct ethanol fuel cells	1134:1158	direct ethanol fuel cells	1134:1158	= 2.2 mWcm-2), suggesting the potential of the newly proposed membranes for application in direct ethanol fuel cells.					
33572312	2	22	theme	reduced	321:327	arg1	oxides					338:343	N-doped reduced graphene oxides	313:343	N-doped reduced graphene oxides	313:343	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	23	dep	Two-dimensional	279:293	arg1	2D					296:297	2D	296:297	2D	296:297	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	23	dep	Two-dimensional	279:293	arg1	oxides					338:343	N-doped reduced graphene oxides	313:343	N-doped reduced graphene oxides	313:343	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	24	theme	polyenaminone	420:432	arg1	N-pEAO					445:450	N-pEAO	445:450	N-pEAO	445:450	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	24	theme	polyenaminone	420:432	arg1	particles					434:442	3D porous N-doped graphitic polyenaminone particles	392:442	3D porous N-doped graphitic polyenaminone particles (N-pEAO)	392:451	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	25	theme	N-doped	313:319	arg1	oxides					338:343	N-doped reduced graphene oxides	313:343	N-doped reduced graphene oxides	313:343	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	0	26	theme	Graphene	42:49	arg1	Composite					57:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	2	27	theme	porous	395:400	arg1	N-pEAO					445:450	N-pEAO	445:450	N-pEAO	445:450	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	27	theme	porous	395:400	arg1	particles					434:442	3D porous N-doped graphitic polyenaminone particles	392:442	3D porous N-doped graphitic polyenaminone particles (N-pEAO)	392:451	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	28	theme	N-doped	402:408	arg1	N-pEAO					445:450	N-pEAO	445:450	N-pEAO	445:450	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	28	theme	N-doped	402:408	arg1	particles					434:442	3D porous N-doped graphitic polyenaminone particles	392:442	3D porous N-doped graphitic polyenaminone particles (N-pEAO)	392:451	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	5	29	theme	=	970:970	arg1	mWcm-2					976:981	= 3.7 mWcm-2)	970:982	= 3.7 mWcm-2)	970:982	= 3.7 mWcm-2) outperformed the pristine CS membrane significantly (Pmax.					
33572312	2	30	theme	quasi	304:308	arg1	1D					310:311	quasi 1D	304:311	quasi 1D	304:311	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	3	31	theme	ethanol	850:856	arg1	permeability					858:869	the ethanol permeability	846:869	(by almost 10-fold) the ethanol permeability	826:869	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	2	32	theme	3D	392:393	arg1	N-pEAO					445:450	N-pEAO	445:450	N-pEAO	445:450	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	32	theme	3D	392:393	arg1	particles					434:442	3D porous N-doped graphitic polyenaminone particles	392:442	3D porous N-doped graphitic polyenaminone particles (N-pEAO)	392:451	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	0	33	theme	Oxide	51:55	arg1	Composite					57:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	2	34	theme	graphitic	512:520	arg1	structure					522:530	their graphitic structure	506:530	their graphitic structure	506:530	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	35	theme	graphitic	410:418	arg1	N-pEAO					445:450	N-pEAO	445:450	N-pEAO	445:450	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	35	theme	graphitic	410:418	arg1	particles					434:442	3D porous N-doped graphitic polyenaminone particles	392:442	3D porous N-doped graphitic polyenaminone particles (N-pEAO)	392:451	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	3	36	dep	impacted	685:692	arg1	increasing					815:824	increasing	815:824	increasing (by almost 10-fold) the ethanol permeability	815:869	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	3	36	dep	impacted	685:692	arg1	reducing					743:750	reducing	743:750	reducing the crystallinity, tensile properties, and the KOH uptake	743:808	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	2	37	dep	synthesized	459:469	arg1	morphology					533:542	morphology	533:542	morphology	533:542	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	2	37	dep	synthesized	459:469	arg1	nitrogen					549:556	nitrogen	549:556	nitrogen (pyridinic, pyrrolic, and quaternary or graphitic)	549:607	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	6	38	theme	membranes	1105:1113	arg1	potential					1073:1081	the potential	1069:1081	the potential of the newly proposed membranes for application in direct ethanol fuel cells	1069:1158	= 2.2 mWcm-2), suggesting the potential of the newly proposed membranes for application in direct ethanol fuel cells.					
33572312	5	39	theme	pristine	1001:1008	arg1	membrane					1013:1020	the pristine CS membrane	997:1020	the pristine CS membrane	997:1020	= 3.7 mWcm-2) outperformed the pristine CS membrane significantly (Pmax.					
33572312	4	40	theme	CS/N	933:936	arg1	rGONRs					938:943	CS/N rGONRs	933:943	CS/N rGONRs (0.07 %) membrane (Pmax	933:967	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	4	40	theme	CS/N	933:936	arg1	%					951:951	0.07 %	946:951	0.07 %	946:951	Within direct alkaline ethanol test cells, it was found that CS/N rGONRs (0.07 %) membrane (Pmax.					
33572312	3	41	theme	CS	716:717	arg1	membrane					719:726	the CS membrane	712:726	the CS membrane	712:726	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	5	42	theme	CS	1010:1011	arg1	membrane					1013:1020	the pristine CS membrane	997:1020	the pristine CS membrane	997:1020	= 3.7 mWcm-2) outperformed the pristine CS membrane significantly (Pmax.					
33572312	6	43	theme	proposed	1096:1103	arg1	membranes					1105:1113	the newly proposed membranes	1086:1113	the newly proposed membranes	1086:1113	= 2.2 mWcm-2), suggesting the potential of the newly proposed membranes for application in direct ethanol fuel cells.					
33572312	3	44	theme	membrane	719:726	arg1	morphology					698:707	the morphology	694:707	the morphology of the CS membrane	694:726	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	2	45	dep	characterized	475:487	arg1	confirm					498:504	confirm	498:504	characterized fully to confirm their graphitic structure	475:530	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	5	46	dep	=	970:970	arg1	3.7					972:974	3.7	972:974	3.7	972:974	= 3.7 mWcm-2) outperformed the pristine CS membrane significantly (Pmax.					
33572312	2	47	theme	graphene	329:336	arg1	oxides					338:343	N-doped reduced graphene oxides	313:343	N-doped reduced graphene oxides	313:343	Two-dimensional (2D) and quasi 1D N-doped reduced graphene oxides (N-rGO) and nanoribbons (N-rGONRs), as well as 3D porous N-doped graphitic polyenaminone particles (N-pEAO), were synthesized and characterized fully to confirm their graphitic structure, morphology, and nitrogen (pyridinic, pyrrolic, and quaternary or graphitic) group contents.					
33572312	6	48	from	application	1119:1129	arg1	cells					1154:1158	direct ethanol fuel cells	1134:1158	direct ethanol fuel cells	1134:1158	= 2.2 mWcm-2), suggesting the potential of the newly proposed membranes for application in direct ethanol fuel cells.					
33572312	0	49	theme	Alkaline	88:95	arg1	Fuel					105:108	Direct Alkaline Ethanol Fuel	81:108	Direct Alkaline Ethanol Fuel Cells	81:114	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	3	50	theme	largest	629:635	arg1	loading					645:651	The largest (0.07%) loading	625:651	The largest (0.07%) loading of N-doped graphene derivatives	625:683	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	3	51	theme	KOH	799:801	arg1	uptake					803:808	the KOH uptake	795:808	the KOH uptake	795:808	The largest (0.07%) loading of N-doped graphene derivatives impacted the morphology of the CS membrane significantly, reducing the crystallinity, tensile properties, and the KOH uptake, and increasing (by almost 10-fold) the ethanol permeability.					
33572312	1	52	theme	different	239:247	arg1	derivatives					266:276	three compositionally and structurally different N-doped graphene derivatives	200:276	three compositionally and structurally different N-doped graphene derivatives	200:276	Herein, we prepared a series of nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives.					
33572312	0	53	theme	Direct	81:86	arg1	Fuel					105:108	Direct Alkaline Ethanol Fuel	81:108	Direct Alkaline Ethanol Fuel Cells	81:114	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	0	54	theme	Reduced	34:40	arg1	Composite					57:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite	0:65	Efficient Chitosan/Nitrogen-Doped Reduced Graphene Oxide Composite Membranes for Direct Alkaline Ethanol Fuel Cells.					
33572312	1	55	theme	nanocomposite	149:161	arg1	membranes					163:171	nanocomposite membranes	149:171	nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives	149:276	Herein, we prepared a series of nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives.					
33572312	1	56	theme	N-doped	249:255	arg1	derivatives					266:276	three compositionally and structurally different N-doped graphene derivatives	200:276	three compositionally and structurally different N-doped graphene derivatives	200:276	Herein, we prepared a series of nanocomposite membranes based on chitosan (CS) and three compositionally and structurally different N-doped graphene derivatives.					
33743445	2	0	theme	concentrations	349:362	arg1	effect					329:334	The effect	325:334	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %)	325:465	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	6	1	theme	of	1054:1055	arg1	0.099					1043:1047	0.099	1043:1047	0.099	1043:1047	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	1	theme	of	1054:1055	arg1	%					1062:1062	EE% of 57.4 %	1050:1062	EE% of 57.4 %	1050:1062	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	10	2	theme	delivery	1899:1906	arg1	systems					1908:1914	MTZ drug delivery systems	1890:1914	MTZ drug delivery systems	1890:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	8	3	theme	kinetic	1463:1469	arg1	modeling					1471:1478	The kinetic modeling	1459:1478	The kinetic modeling of drug release	1459:1494	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	3	4	theme	MTZ-loaded	508:517	arg1	NPs					522:524	MTZ-loaded CS NPs	508:524	MTZ-loaded CS NPs	508:524	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	8	5	theme	drug	1530:1533	arg1	diffusion					1517:1525	the Fickian diffusion	1505:1525	the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1505:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	1	6	theme	ionic	275:279	arg1	technique					290:298	the ionic gelation technique	271:298	the ionic gelation technique	271:298	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	6	7	theme	EE	1050:1051	arg1	0.099					1043:1047	0.099	1043:1047	0.099	1043:1047	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	7	theme	EE	1050:1051	arg1	%					1062:1062	EE% of 57.4 %	1050:1062	EE% of 57.4 %	1050:1062	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	4	8	theme	drug	616:619	arg1	release					621:627	drug release	616:627	drug release at pH = 7.4	616:639	Furthermore, drug release at pH = 7.4 was examined and the corresponding mechanism was explored.					
33743445	10	9	theme	CS	1770:1771	arg1	NPs					1773:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	9	theme	CS	1770:1771	arg1	form					1871:1874	an acceptable nanoparticle form	1844:1874	an acceptable nanoparticle form for designing MTZ drug delivery systems	1844:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	0	10	theme	Enhanced	0:7	arg1	activity					23:30	Enhanced antibacterial activity	0:30	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles	0:75	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	6	11	theme	0.099	1043:1047	arg1	potential					1074:1082	zeta potential	1069:1082	zeta potential of +46.32 mV	1069:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	11	theme	0.099	1043:1047	arg1	PDI					1036:1038	PDI	1036:1038	PDI of 0.099, EE% of 57.4 %	1036:1062	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	11	theme	0.099	1043:1047	arg1	nm					1032:1033	284 nm	1028:1033	284 nm	1028:1033	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	12	theme	mV	1094:1095	arg1	potential					1074:1082	zeta potential	1069:1082	zeta potential of +46.32 mV	1069:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	12	theme	mV	1094:1095	arg1	PDI					1036:1038	PDI	1036:1038	PDI of 0.099, EE% of 57.4 %	1036:1062	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	12	theme	mV	1094:1095	arg1	nm					1032:1033	284 nm	1028:1033	284 nm	1028:1033	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	8	13	theme	Korsmeyer-Peppas	1548:1563	arg1	model					1565:1569	the Korsmeyer-Peppas model	1544:1569	the Korsmeyer-Peppas model	1544:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	1	14	theme	CS	212:213	arg1	nanoparticles					197:209	the salt-assisted chitosan nanoparticles	170:209	the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ)	170:249	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	1	14	theme	CS	212:213	arg1	NPs					215:217	CS NPs	212:217	CS NPs	212:217	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	6	15	theme	zeta	1069:1072	arg1	potential					1074:1082	zeta potential	1069:1082	zeta potential of +46.32 mV	1069:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	16	theme	PDI	1036:1038	arg1	size					1020:1023	the particle size	1007:1023	the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV	1007:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	0	17	theme	fragilis	145:152	arg1	bacterium					120:128	anaerobic bacterium	110:128	anaerobic bacterium of Bacteroides fragilis	110:152	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	3	18	theme	NPs	522:524	arg1	stability					495:503	the stability	491:503	the stability of MTZ-loaded CS NPs in the absence/presence of NaCl	491:556	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	8	19	theme	in-vitro	1289:1296	arg1	profiles					1311:1318	The in-vitro drug release profiles	1285:1318	The in-vitro drug release profiles	1285:1318	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	7	20	theme	surface	1220:1226	arg1	density					1235:1241	higher surface charge density	1213:1241	higher surface charge density calculated using the Gouy-Chapman theory	1213:1282	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	6	21	theme	optimum	974:980	arg1	NPs					982:984	the optimum NPs	970:984	the optimum NPs	970:984	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	8	22	from	properties	1430:1439	arg1	pH.					1455:1457	high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1450:1569	high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1450:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	8	23	theme	release	1303:1309	arg1	profiles					1311:1318	The in-vitro drug release profiles	1285:1318	The in-vitro drug release profiles	1285:1318	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	6	24	theme	potential	1074:1082	arg1	size					1020:1023	the particle size	1007:1023	the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV	1007:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	25	theme	particle	1011:1018	arg1	size					1020:1023	the particle size	1007:1023	the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV	1007:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	10	26	theme	proper	1807:1812	arg1	concentration					1814:1826	a proper concentration	1805:1826	a proper concentration of NaCl	1805:1834	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	4	27	theme	corresponding	662:674	arg1	mechanism					676:684	the corresponding mechanism	658:684	the corresponding mechanism	658:684	Furthermore, drug release at pH = 7.4 was examined and the corresponding mechanism was explored.					
33743445	3	28	theme	6-month	579:585	arg1	period					595:600	a 6-month storage period	577:600	a 6-month storage period	577:600	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	0	29	theme	nanoparticles	63:75	arg1	activity					23:30	Enhanced antibacterial activity	0:30	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles	0:75	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	5	30	theme	minimum	836:842	arg1	MIC					870:872	MIC	870:872	MIC	870:872	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	5	30	theme	minimum	836:842	arg1	concentration					855:867	the minimum inhibitory concentration	832:867	the minimum inhibitory concentration (MIC)	832:873	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	8	31	theme	drug	1351:1354	arg1	release					1356:1362	a more controlled drug release	1333:1362	a more controlled drug release of MTZ from CS NPs	1333:1381	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	3	32	from	stability	495:503	arg1	absence/presence					533:548	the absence/presence	529:548	the absence/presence of NaCl	529:556	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	5	33	theme	time-kill	713:721	arg1	assay					723:727	the time-kill assay	709:727	the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis	709:804	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	8	34	dep	pH.	1455:1457	arg1	approved					1496:1503	approved	1496:1503	approved	1496:1503	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	1	35	theme	salt-assisted	174:186	arg1	nanoparticles					197:209	the salt-assisted chitosan nanoparticles	170:209	the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ)	170:249	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	1	35	theme	salt-assisted	174:186	arg1	NPs					215:217	CS NPs	212:217	CS NPs	212:217	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	10	36	dep	concentration	1814:1826	arg1	presence					1793:1800	presence	1793:1800	presence	1793:1800	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	36	dep	concentration	1814:1826	arg1	the					1789:1791	the	1789:1791	the	1789:1791	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	37	theme	acceptable	1847:1856	arg1	NPs					1773:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	37	theme	acceptable	1847:1856	arg1	form					1871:1874	an acceptable nanoparticle form	1844:1874	an acceptable nanoparticle form for designing MTZ drug delivery systems	1844:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	0	38	theme	anaerobic	110:118	arg1	bacterium					120:128	anaerobic bacterium	110:128	anaerobic bacterium of Bacteroides fragilis	110:152	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	9	39	theme	antibacterial	1613:1625	arg1	activity					1627:1634	the higher antibacterial activity	1602:1634	the higher antibacterial activity of salt-assisted MZ-loaded CS NPs	1602:1668	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	2	40	theme	zeta	390:393	arg1	potential					395:403	zeta potential	390:403	zeta potential	390:403	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	5	41	theme	salt-assisted	745:757	arg1	CS					770:771	salt-assisted MTZ-loaded CS	745:771	salt-assisted MTZ-loaded CS	745:771	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	1	42	theme	gelation	281:288	arg1	technique					290:298	the ionic gelation technique	271:298	the ionic gelation technique	271:298	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	5	43	theme	bactericidal	887:898	arg1	concentration					900:912	minimum bactericidal concentration	879:912	minimum bactericidal concentration (MBC)	879:918	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	5	43	theme	bactericidal	887:898	arg1	MBC					915:917	MBC	915:917	MBC	915:917	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	7	44	theme	enhancement	1167:1177	arg1	stability					1103:1111	More stability	1098:1111	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time,	1098:1190	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	9	45	theme	MZ-loaded	1653:1661	arg1	NPs					1666:1668	salt-assisted MZ-loaded CS NPs	1639:1668	salt-assisted MZ-loaded CS NPs	1639:1668	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	5	46	theme	CS	770:771	arg1	NPs					773:775	free MTZ and salt-assisted MTZ-loaded CS NPs	732:775	free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis	732:804	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	7	47	theme	Gouy-Chapman	1264:1275	arg1	theory					1277:1282	the Gouy-Chapman theory	1260:1282	the Gouy-Chapman theory	1260:1282	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	7	48	theme	lower	1156:1160	arg1	enhancement					1167:1177	lower size enhancement	1156:1177	lower size enhancement	1156:1177	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	9	49	theme	NPs	1666:1668	arg1	activity					1627:1634	the higher antibacterial activity	1602:1634	the higher antibacterial activity of salt-assisted MZ-loaded CS NPs	1602:1668	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	0	50	theme	uniform	35:41	arg1	nanoparticles					63:75	uniform and stable chitosan nanoparticles	35:75	uniform and stable chitosan nanoparticles	35:75	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	8	51	from	NPs	1379:1381	arg1	release					1356:1362	a more controlled drug release	1333:1362	a more controlled drug release of MTZ from CS NPs	1333:1381	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	6	52	theme	mM	961:962	arg1	NaCl					964:967	20 mM NaCl	958:967	20 mM NaCl	958:967	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	2	53	theme	entrapment	438:447	arg1	efficiency					449:458	entrapment efficiency	438:458	entrapment efficiency (EE %)	438:465	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	53	theme	entrapment	438:447	arg1	%					464:464	EE %	461:464	EE %	461:464	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	54	theme	different	339:347	arg1	concentrations					349:362	different concentrations	339:362	different concentrations of NaCl	339:370	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	5	55	theme	MTZ	737:739	arg1	NPs					773:775	free MTZ and salt-assisted MTZ-loaded CS NPs	732:775	free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis	732:804	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	1	56	theme	NaCl	319:322	arg1	presence					307:314	the presence	303:314	the presence of NaCl	303:322	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	9	57	theme	free	1686:1689	arg1	MTZ					1691:1693	the free MTZ	1682:1693	the free MTZ against B. fragilis bacterium	1682:1723	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	1	58	contain	containing	220:229	arg2	metronidazole					231:243	metronidazole	231:243	metronidazole (MTZ)	231:249	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	1	58	contain	containing	220:229	arg2	MTZ					246:248	MTZ	246:248	MTZ	246:248	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	1	58	contain	containing	220:229	arg1	nanoparticles					197:209	the salt-assisted chitosan nanoparticles	170:209	the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ)	170:249	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	1	58	contain	containing	220:229	arg1	NPs					215:217	CS NPs	212:217	CS NPs	212:217	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	7	59	theme	salt-assisted	1116:1128	arg1	nanoparticles					1130:1142	salt-assisted nanoparticles	1116:1142	salt-assisted nanoparticles	1116:1142	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	8	60	theme	drug	1483:1486	arg1	release					1488:1494	drug release	1483:1494	drug release	1483:1494	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	0	61	theme	chitosan	54:61	arg1	nanoparticles					63:75	uniform and stable chitosan nanoparticles	35:75	uniform and stable chitosan nanoparticles	35:75	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	8	62	theme	CS	1444:1445	arg1	properties					1430:1439	the shrinkage properties	1416:1439	the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1416:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	4	63	from	7.4	637:639	arg1	release					621:627	drug release	616:627	drug release at pH = 7.4	616:639	Furthermore, drug release at pH = 7.4 was examined and the corresponding mechanism was explored.					
33743445	2	64	from	effect	329:334	arg1	PDI					428:430	PDI	428:430	PDI	428:430	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	64	from	effect	329:334	arg1	%					464:464	EE %	461:464	EE %	461:464	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	64	from	effect	329:334	arg1	potential					395:403	zeta potential	390:403	zeta potential	390:403	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	64	from	effect	329:334	arg1	index					421:425	polydispersity index	406:425	polydispersity index (PDI)	406:431	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	64	from	effect	329:334	arg1	efficiency					449:458	entrapment efficiency	438:458	entrapment efficiency (EE %)	438:465	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	64	from	effect	329:334	arg1	size					384:387	particle size	375:387	particle size	375:387	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	8	65	theme	Fickian	1509:1515	arg1	diffusion					1517:1525	the Fickian diffusion	1505:1525	the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1505:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	6	66	theme	%	1052:1052	arg1	0.099					1043:1047	0.099	1043:1047	0.099	1043:1047	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	6	66	theme	%	1052:1052	arg1	%					1062:1062	EE% of 57.4 %	1050:1062	EE% of 57.4 %	1050:1062	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	7	67	theme	nanoparticles	1130:1142	arg1	stability					1103:1111	More stability	1098:1111	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time,	1098:1190	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	1	68	theme	chitosan	188:195	arg1	nanoparticles					197:209	the salt-assisted chitosan nanoparticles	170:209	the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ)	170:249	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	1	68	theme	chitosan	188:195	arg1	NPs					215:217	CS NPs	212:217	CS NPs	212:217	In this paper, the salt-assisted chitosan nanoparticles (CS NPs) containing metronidazole (MTZ) were prepared using the ionic gelation technique in the presence of NaCl.					
33743445	0	69	theme	stable	47:52	arg1	nanoparticles					63:75	uniform and stable chitosan nanoparticles	35:75	uniform and stable chitosan nanoparticles	35:75	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	10	70	theme	MTZ-loaded	1759:1768	arg1	NPs					1773:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	70	theme	MTZ-loaded	1759:1768	arg1	form					1871:1874	an acceptable nanoparticle form	1844:1874	an acceptable nanoparticle form for designing MTZ drug delivery systems	1844:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	3	71	theme	CS	519:520	arg1	NPs					522:524	MTZ-loaded CS NPs	508:524	MTZ-loaded CS NPs	508:524	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	5	72	theme	NPs	773:775	arg1	assay					723:727	the time-kill assay	709:727	the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis	709:804	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	8	73	theme	drug	1298:1301	arg1	profiles					1311:1318	The in-vitro drug release profiles	1285:1318	The in-vitro drug release profiles	1285:1318	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	3	74	theme	NaCl	553:556	arg1	absence/presence					533:548	the absence/presence	529:548	the absence/presence of NaCl	529:556	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	5	75	theme	Bacteroides	785:795	arg1	fragilis					797:804	Bacteroides fragilis	785:804	Bacteroides fragilis	785:804	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	10	76	theme	drug	1894:1897	arg1	systems					1908:1914	MTZ drug delivery systems	1890:1914	MTZ drug delivery systems	1890:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	7	77	theme	charge	1228:1233	arg1	density					1235:1241	higher surface charge density	1213:1241	higher surface charge density calculated using the Gouy-Chapman theory	1213:1282	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	7	78	theme	higher	1213:1218	arg1	density					1235:1241	higher surface charge density	1213:1241	higher surface charge density calculated using the Gouy-Chapman theory	1213:1282	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	8	79	from	pH.	1455:1457	arg1	CS					1444:1445	CS	1444:1445	CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1444:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	8	79	from	pH.	1455:1457	arg1	properties					1430:1439	the shrinkage properties	1416:1439	the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1416:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	6	80	theme	20	958:959	arg1	mM					961:962	mM	961:962	mM	961:962	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	8	81	theme	controlled	1340:1349	arg1	release					1356:1362	a more controlled drug release	1333:1362	a more controlled drug release of MTZ from CS NPs	1333:1381	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	9	82	theme	time-kill	1576:1584	arg1	plots					1586:1590	The time-kill plots	1572:1590	The time-kill plots	1572:1590	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	10	83	theme	NaCl	1831:1834	arg1	concentration					1814:1826	a proper concentration	1805:1826	a proper concentration of NaCl	1805:1834	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	5	84	theme	inhibitory	844:853	arg1	MIC					870:872	MIC	870:872	MIC	870:872	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	5	84	theme	inhibitory	844:853	arg1	concentration					855:867	the minimum inhibitory concentration	832:867	the minimum inhibitory concentration (MIC)	832:873	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	2	85	theme	EE	461:462	arg1	efficiency					449:458	entrapment efficiency	438:458	entrapment efficiency (EE %)	438:465	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	85	theme	EE	461:462	arg1	%					464:464	EE %	461:464	EE %	461:464	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	8	86	theme	MTZ	1367:1369	arg1	release					1356:1362	a more controlled drug release	1333:1362	a more controlled drug release of MTZ from CS NPs	1333:1381	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	10	87	theme	nanoparticle	1858:1869	arg1	NPs					1773:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	87	theme	nanoparticle	1858:1869	arg1	form					1871:1874	an acceptable nanoparticle form	1844:1874	an acceptable nanoparticle form for designing MTZ drug delivery systems	1844:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	9	88	dep	B.	1703:1704	arg1	fragilis					1706:1713	fragilis	1706:1713	fragilis	1706:1713	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	9	89	theme	higher	1606:1611	arg1	activity					1627:1634	the higher antibacterial activity	1602:1634	the higher antibacterial activity of salt-assisted MZ-loaded CS NPs	1602:1668	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	8	90	theme	CS	1376:1377	arg1	NPs					1379:1381	CS NPs	1376:1381	CS NPs	1376:1381	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	0	91	theme	Bacteroides	133:143	arg1	fragilis					145:152	Bacteroides fragilis	133:152	Bacteroides fragilis	133:152	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	0	92	theme	antibacterial	9:21	arg1	activity					23:30	Enhanced antibacterial activity	0:30	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles	0:75	Enhanced antibacterial activity of uniform and stable chitosan nanoparticles containing metronidazole against anaerobic bacterium of Bacteroides fragilis.					
33743445	5	93	theme	minimum	879:885	arg1	concentration					900:912	minimum bactericidal concentration	879:912	minimum bactericidal concentration (MBC)	879:918	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	5	93	theme	minimum	879:885	arg1	MBC					915:917	MBC	915:917	MBC	915:917	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	7	94	theme	size	1162:1165	arg1	enhancement					1167:1177	lower size enhancement	1156:1177	lower size enhancement	1156:1177	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	9	95	theme	salt-assisted	1639:1651	arg1	NPs					1666:1668	salt-assisted MZ-loaded CS NPs	1639:1668	salt-assisted MZ-loaded CS NPs	1639:1668	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	8	96	theme	free	1395:1398	arg1	MTZ					1400:1402	free MTZ	1395:1402	free MTZ	1395:1402	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	2	97	theme	particle	375:382	arg1	size					384:387	particle size	375:387	particle size	375:387	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	5	98	theme	MTZ-loaded	759:768	arg1	CS					770:771	salt-assisted MTZ-loaded CS	745:771	salt-assisted MTZ-loaded CS	745:771	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	9	99	theme	CS	1663:1664	arg1	NPs					1666:1668	salt-assisted MZ-loaded CS NPs	1639:1668	salt-assisted MZ-loaded CS NPs	1639:1668	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	2	100	theme	polydispersity	406:419	arg1	PDI					428:430	PDI	428:430	PDI	428:430	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	2	100	theme	polydispersity	406:419	arg1	index					421:425	polydispersity index	406:425	polydispersity index (PDI)	406:431	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
33743445	6	101	theme	NaCl	964:967	arg1	presence					946:953	the presence	942:953	the presence of 20 mM NaCl	942:967	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	3	102	theme	storage	587:593	arg1	period					595:600	a 6-month storage period	577:600	a 6-month storage period	577:600	Also, the stability of MTZ-loaded CS NPs in the absence/presence of NaCl was evaluated over a 6-month storage period.					
33743445	5	103	theme	free	732:735	arg1	MTZ					737:739	free MTZ	732:739	free MTZ	732:739	Finally, the time-kill assay of free MTZ and salt-assisted MTZ-loaded CS NPs against Bacteroides fragilis was performed by applying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).					
33743445	6	104	theme	nm	1032:1033	arg1	size					1020:1023	the particle size	1007:1023	the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV	1007:1095	It was found that in the presence of 20 mM NaCl, the optimum NPs can be achieved with the particle size of 284 nm, PDI of 0.099, EE% of 57.4 %, and zeta potential of +46.32 mV.					
33743445	8	105	theme	shrinkage	1420:1428	arg1	properties					1430:1439	the shrinkage properties	1416:1439	the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model	1416:1569	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	10	106	from	form	1871:1874	arg1	conclusion					1729:1738	conclusion	1729:1738	conclusion	1729:1738	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	107	theme	MTZ	1890:1892	arg1	systems					1908:1914	MTZ drug delivery systems	1890:1914	MTZ drug delivery systems	1890:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	8	108	theme	release	1488:1494	arg1	modeling					1471:1478	The kinetic modeling	1459:1478	The kinetic modeling of drug release	1459:1494	The in-vitro drug release profiles demonstrated a more controlled drug release of MTZ from CS NPs compared to free MTZ, because of the shrinkage properties of CS at high pH. The kinetic modeling of drug release approved the Fickian diffusion of drug based on the Korsmeyer-Peppas model.					
33743445	7	109	theme	More	1098:1101	arg1	stability					1103:1111	More stability	1098:1111	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time,	1098:1190	More stability of salt-assisted nanoparticles, as well as lower size enhancement versus time, were observed due to higher surface charge density calculated using the Gouy-Chapman theory.					
33743445	9	110	theme	B.	1703:1704	arg1	bacterium					1715:1723	B. fragilis bacterium	1703:1723	B. fragilis bacterium	1703:1723	The time-kill plots confirmed the higher antibacterial activity of salt-assisted MZ-loaded CS NPs compared to the free MTZ against B. fragilis bacterium.					
33743445	10	111	theme	salt-assisted	1745:1757	arg1	NPs					1773:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	the salt-assisted MTZ-loaded CS NPs	1741:1775	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	10	111	theme	salt-assisted	1745:1757	arg1	form					1871:1874	an acceptable nanoparticle form	1844:1874	an acceptable nanoparticle form for designing MTZ drug delivery systems	1844:1914	In conclusion, the salt-assisted MTZ-loaded CS NPs prepared in the presence of a proper concentration of NaCl, can be an acceptable nanoparticle form for designing MTZ drug delivery systems.					
33743445	2	112	theme	NaCl	367:370	arg1	concentrations					349:362	different concentrations	339:362	different concentrations of NaCl	339:370	The effect of different concentrations of NaCl on particle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (EE %) was investigated.					
34294324	0	0	theme	different	95:103	arg1	types					105:109	different types	95:109	different types in comparison	95:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	2	1	theme	cellulose	537:545	arg1	allomorphs					547:556	crystalline cellulose allomorphs I and II	525:565	crystalline cellulose allomorphs I and II	525:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	1	theme	cellulose	537:545	arg1	II					564:565	II	564:565	II	564:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	1	theme	cellulose	537:545	arg1	I					558:558	I	558:558	I	558:558	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	2	with	CNC	516:518	arg1	allomorphs					547:556	crystalline cellulose allomorphs I and II	525:565	crystalline cellulose allomorphs I and II	525:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	2	with	CNC	516:518	arg1	II					564:565	II	564:565	II	564:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	2	with	CNC	516:518	arg1	I					558:558	I	558:558	I	558:558	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	3	theme	crystalline	525:535	arg1	allomorphs					547:556	crystalline cellulose allomorphs I and II	525:565	crystalline cellulose allomorphs I and II	525:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	3	theme	crystalline	525:535	arg1	II					564:565	II	564:565	II	564:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	3	theme	crystalline	525:535	arg1	I					558:558	I	558:558	I	558:558	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	6	4	theme	disc-like	827:835	arg1	particles					837:845	disc-like particles	827:845	disc-like particles	827:845	However, in case of disc-like particles, the kidney mass coefficient noticeably changed.					
34294324	2	5	theme	comparative	380:390	arg1	analysis					407:414	comparative and integrated analysis	380:414	analysis	407:414	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	7	6	theme	cell	966:969	arg1	membrane					971:978	the red cell membrane	958:978	the red cell membrane	958:978	CNC have neither triggered platelet aggregation nor destroyed the red cell membrane.					
34294324	8	7	theme	Intravenous	981:991	arg1	administration					993:1006	Intravenous administration	981:1006	Intravenous administration to rabbits	981:1017	Intravenous administration to rabbits has not affected the plasma clotting time.					
34294324	0	8	theme	types	105:109	arg1	nanocrystals					79:90	acetylated cellulose nanocrystals	58:90	acetylated cellulose nanocrystals of different types in comparison	58:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	1	9	theme	supramolecular	279:292	arg1	structure					294:302	supramolecular structure	279:302	supramolecular structure	279:302	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	2	10	theme	biocompatibility	433:448	arg1	aspects					450:456	the mentioned biocompatibility aspects	419:456	the mentioned biocompatibility aspects of partially acetylated rod-	419:485	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	6	11	theme	mass	859:862	arg1	coefficient					864:874	the kidney mass coefficient	848:874	the kidney mass coefficient	848:874	However, in case of disc-like particles, the kidney mass coefficient noticeably changed.					
34294324	9	12	theme	disc-like	1103:1111	arg1	particles					1113:1121	the disc-like particles	1099:1121	the disc-like particles	1099:1121	Rod-like CNC are more resistant, and the disc-like particles are more susceptible to degradation under the influence of cellulases.					
34294324	9	12	theme	disc-like	1103:1111	arg1	susceptible					1132:1142	susceptible	1132:1142	susceptible	1132:1142	Rod-like CNC are more resistant, and the disc-like particles are more susceptible to degradation under the influence of cellulases.					
34294324	2	13	theme	mentioned	423:431	arg1	aspects					450:456	the mentioned biocompatibility aspects	419:456	the mentioned biocompatibility aspects of partially acetylated rod-	419:485	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	14	theme	disc-lake	492:500	arg1	morphology					502:511	disc-lake morphology	492:511	disc-lake morphology of CNC with crystalline cellulose allomorphs I and II	492:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	5	15	theme	internal	774:781	arg1	organs					783:788	internal organs	774:788	internal organs	774:788	Morphology of internal organs has not changed.					
34294324	4	16	theme	CNC	702:704	arg1	types					686:690	all types	682:690	all types of tested CNC	682:704	The aqueous solution of all types of tested CNC has not been toxic to mice after oral administration.					
34294324	8	17	theme	clotting	1047:1054	arg1	time					1056:1059	the plasma clotting time	1036:1059	the plasma clotting time	1036:1059	Intravenous administration to rabbits has not affected the plasma clotting time.					
34294324	3	18	theme	CNC	611:613	arg1	study					602:606	the study	598:606	the study of CNC obtained from the sulfuric acid solutions	598:655	These data have also included the study of CNC obtained from the sulfuric acid solutions.					
34294324	4	19	theme	tested	695:700	arg1	CNC					702:704	tested CNC	695:704	tested CNC	695:704	The aqueous solution of all types of tested CNC has not been toxic to mice after oral administration.					
34294324	8	20	theme	plasma	1040:1045	arg1	time					1056:1059	the plasma clotting time	1036:1059	the plasma clotting time	1036:1059	Intravenous administration to rabbits has not affected the plasma clotting time.					
34294324	2	21	theme	acetylated	471:480	arg1	rod-					482:485	partially acetylated rod-	461:485	partially acetylated rod-	461:485	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	22	theme	rod-	482:485	arg1	aspects					450:456	the mentioned biocompatibility aspects	419:456	the mentioned biocompatibility aspects of partially acetylated rod-	419:485	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	4	23	theme	aqueous	662:668	arg1	solution					670:677	The aqueous solution	658:677	The aqueous solution of all types of tested CNC	658:704	The aqueous solution of all types of tested CNC has not been toxic to mice after oral administration.					
34294324	7	24	theme	platelet	923:930	arg1	aggregation					932:942	platelet aggregation	923:942	platelet aggregation	923:942	CNC have neither triggered platelet aggregation nor destroyed the red cell membrane.					
34294324	2	25	theme	CNC	516:518	arg1	morphology					502:511	disc-lake morphology	492:511	disc-lake morphology of CNC with crystalline cellulose allomorphs I and II	492:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	25	theme	CNC	516:518	arg1	analysis					407:414	comparative and integrated analysis	380:414	analysis	407:414	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	0	26	theme	acute	40:44	arg1	toxicity					46:53	acute toxicity	40:53	acute toxicity	40:53	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	0	27	from	nanocrystals	79:90	arg1	comparison					114:123	comparison	114:123	comparison	114:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	3	28	theme	sulfuric	633:640	arg1	solutions					647:655	the sulfuric acid solutions	629:655	the sulfuric acid solutions	629:655	These data have also included the study of CNC obtained from the sulfuric acid solutions.					
34294324	2	29	theme	aspects	450:456	arg1	morphology					502:511	disc-lake morphology	492:511	disc-lake morphology of CNC with crystalline cellulose allomorphs I and II	492:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	29	theme	aspects	450:456	arg1	analysis					407:414	comparative and integrated analysis	380:414	analysis	407:414	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	0	30	theme	acetylated	58:67	arg1	nanocrystals					79:90	acetylated cellulose nanocrystals	58:90	acetylated cellulose nanocrystals of different types in comparison	58:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	5	31	theme	organs	783:788	arg1	Morphology					760:769	Morphology	760:769	Morphology of internal organs	760:788	Morphology of internal organs has not changed.					
34294324	1	32	with	relationship	206:217	arg1	toxicity					309:316	toxicity	309:316	toxicity	309:316	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	1	32	with	relationship	206:217	arg1	hemocompatibility					319:335	hemocompatibility	319:335	hemocompatibility	319:335	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	1	32	with	relationship	206:217	arg1	biodegradability					342:357	biodegradability	342:357	biodegradability	342:357	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	6	33	theme	kidney	852:857	arg1	coefficient					864:874	the kidney mass coefficient	848:874	the kidney mass coefficient	848:874	However, in case of disc-like particles, the kidney mass coefficient noticeably changed.					
34294324	7	34	theme	red	962:964	arg1	membrane					971:978	the red cell membrane	958:978	the red cell membrane	958:978	CNC have neither triggered platelet aggregation nor destroyed the red cell membrane.					
34294324	0	35	from	comparison	114:123	arg1	nanocrystals					79:90	acetylated cellulose nanocrystals	58:90	acetylated cellulose nanocrystals of different types in comparison	58:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	1	36	theme	promising	139:147	arg1	CNC					173:175	CNC	173:175	CNC	173:175	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	1	36	theme	promising	139:147	arg1	nanocrystals					159:170	promising cellulose nanocrystals	139:170	promising cellulose nanocrystals (CNC)	139:176	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	2	37	dep	allomorphs	547:556	arg1	allomorphs					547:556	crystalline cellulose allomorphs I and II	525:565	crystalline cellulose allomorphs I and II	525:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	37	dep	allomorphs	547:556	arg1	II					564:565	II	564:565	II	564:565	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	2	37	dep	allomorphs	547:556	arg1	I					558:558	I	558:558	I	558:558	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	1	38	theme	cellulose	149:157	arg1	CNC					173:175	CNC	173:175	CNC	173:175	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	1	38	theme	cellulose	149:157	arg1	nanocrystals					159:170	promising cellulose nanocrystals	139:170	promising cellulose nanocrystals (CNC)	139:176	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	9	39	theme	Rod-like	1062:1069	arg1	CNC					1071:1073	Rod-like CNC	1062:1073	Rod-like CNC	1062:1073	Rod-like CNC are more resistant, and the disc-like particles are more susceptible to degradation under the influence of cellulases.					
34294324	4	40	theme	oral	739:742	arg1	administration					744:757	oral administration	739:757	oral administration	739:757	The aqueous solution of all types of tested CNC has not been toxic to mice after oral administration.					
34294324	0	41	theme	nanocrystals	79:90	arg1	biodegradability					19:34	biodegradability	19:34	biodegradability	19:34	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	0	41	theme	nanocrystals	79:90	arg1	toxicity					46:53	acute toxicity	40:53	acute toxicity	40:53	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	0	41	theme	nanocrystals	79:90	arg1	Hemocompatibility					0:16	Hemocompatibility	0:16	Hemocompatibility	0:16	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	0	42	from	types	105:109	arg1	comparison					114:123	comparison	114:123	comparison	114:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	3	43	theme	acid	642:645	arg1	solutions					647:655	the sulfuric acid solutions	629:655	the sulfuric acid solutions	629:655	These data have also included the study of CNC obtained from the sulfuric acid solutions.					
34294324	9	44	theme	cellulases	1182:1191	arg1	influence					1169:1177	the influence	1165:1177	the influence of cellulases	1165:1191	Rod-like CNC are more resistant, and the disc-like particles are more susceptible to degradation under the influence of cellulases.					
34294324	1	45	theme	nanocrystals	159:170	arg1	Promotion					126:134	Promotion	126:134	Promotion of promising cellulose nanocrystals (CNC)	126:176	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	4	46	theme	types	686:690	arg1	solution					670:677	The aqueous solution	658:677	The aqueous solution of all types of tested CNC	658:704	The aqueous solution of all types of tested CNC has not been toxic to mice after oral administration.					
34294324	1	47	theme	surface	245:251	arg1	composition					262:272	surface chemical composition	245:272	surface chemical composition	245:272	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34294324	0	48	theme	cellulose	69:77	arg1	nanocrystals					79:90	acetylated cellulose nanocrystals	58:90	acetylated cellulose nanocrystals of different types in comparison	58:123	Hemocompatibility, biodegradability and acute toxicity of acetylated cellulose nanocrystals of different types in comparison.					
34294324	2	49	theme	integrated	396:405	arg1	analysis					407:414	comparative and integrated analysis	380:414	analysis	407:414	This paper outlines comparative and integrated analysis of the mentioned biocompatibility aspects of partially acetylated rod-, and disc-lake morphology of CNC with crystalline cellulose allomorphs I and II.					
34294324	1	50	theme	chemical	253:260	arg1	composition					262:272	surface chemical composition	245:272	surface chemical composition	245:272	Promotion of promising cellulose nanocrystals (CNC) is largely dependent on the relationship between their morphology, surface chemical composition, and supramolecular structure with toxicity, hemocompatibility, and biodegradability.					
34822203	6	0	theme	Nanoindentation	997:1011	arg1	testing					1013:1019	Nanoindentation testing	997:1019	Nanoindentation testing	997:1019	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	9	1	theme	rodent	1736:1741	arg1	model					1743:1747	the rodent model	1732:1747	the rodent model	1732:1747	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	6	2	contain	had	1047:1049	arg2	modulus					1073:1079	a smaller compressive modulus	1051:1079	a smaller compressive modulus	1051:1079	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	6	2	contain	had	1047:1049	arg1	group					1041:1045	the old group	1033:1045	the old group	1033:1045	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	6	3	theme	insertion	1088:1096	arg1	site					1098:1101	the insertion site	1084:1101	the insertion site	1084:1101	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	4	4	theme	newborn	751:757	arg1	group					759:763	the newborn group	747:763	the newborn group	747:763	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	3	5	theme	different	473:481	arg1	mice					497:500	mice	497:500	mice (newborn, young, adult, and old)	497:533	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	3	5	theme	different	473:481	arg1	groups					487:492	Four different age groups	468:492	Four different age groups of mice (newborn, young, adult, and old)	468:533	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	5	6	theme	compositional	770:782	arg1	features					801:808	The compositional and histological features	766:808	The compositional and histological features of young, adult, and old groups	766:840	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	6	7	theme	compressive	1061:1071	arg1	modulus					1073:1079	a smaller compressive modulus	1051:1079	a smaller compressive modulus	1051:1079	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	4	8	theme	entheses	576:583	arg1	variations					558:567	Striking variations	549:567	Striking variations of the entheses	549:583	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	5	9	theme	histological	788:799	arg1	features					801:808	The compositional and histological features	766:808	The compositional and histological features of young, adult, and old groups	766:840	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	9	10	theme	young	1590:1594	arg1	period					1601:1606	the young time period	1586:1606	the young time period	1586:1606	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	6	11	theme	other	1122:1126	arg1	groups					1128:1133	other groups	1122:1133	other groups	1122:1133	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	3	12	theme	mice	497:500	arg1	mice					497:500	mice	497:500	mice (newborn, young, adult, and old)	497:533	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	3	12	theme	mice	497:500	arg1	groups					487:492	Four different age groups	468:492	Four different age groups of mice (newborn, young, adult, and old)	468:533	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	8	13	from	differences	1340:1350	arg1	content					1363:1369	protein content	1355:1369	protein content	1355:1369	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	4	14	theme	Striking	549:556	arg1	variations					558:567	Striking variations	549:567	Striking variations of the entheses	549:583	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	5	15	theme	old	986:988	arg1	group					990:994	the old group	982:994	the old group	982:994	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	8	16	theme	newborn	1379:1385	arg1	groups					1397:1402	newborn and young groups	1379:1402	newborn and young groups	1379:1402	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	8	17	theme	young	1391:1395	arg1	groups					1397:1402	newborn and young groups	1379:1402	newborn and young groups	1379:1402	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	4	18	theme	matured	629:635	arg1	groups					637:642	the newborn and other matured groups	607:642	the newborn and other matured groups	607:642	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	5	19	theme	insertion	963:971	arg1	sites					973:977	the insertion sites	959:977	the insertion sites in the old group	959:994	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	5	20	theme	old	831:833	arg1	groups					835:840	young, adult, and old groups	813:840	young, adult, and old groups	813:840	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	9	21	theme	increased	1617:1625	arg1	risk					1627:1630	the increased risk	1613:1630	the increased risk of rotator cuff tendon injuries in the elderly population	1613:1688	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	2	22	from	model	461:465	arg1	alterations					383:393	the histological, molecular, and biomechanical alterations	336:393	the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model	336:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	5	23	from	sites	973:977	arg1	group					990:994	the old group	982:994	the old group	982:994	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	3	24	dep	mice	497:500	arg1	adult					519:523	adult	519:523	adult	519:523	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	3	24	dep	mice	497:500	arg1	old					530:532	old	530:532	old	530:532	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	3	24	dep	mice	497:500	arg1	young					512:516	young	512:516	young	512:516	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	3	24	dep	mice	497:500	arg1	newborn					503:509	newborn	503:509	newborn	503:509	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	9	25	theme	cuff	1643:1646	arg1	injuries					1655:1662	rotator cuff tendon injuries	1635:1662	rotator cuff tendon injuries	1635:1662	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	8	26	theme	young	1428:1432	arg1	groups					1450:1455	young, adult, and old groups	1428:1455	young, adult, and old groups	1428:1455	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	2	27	theme	mouse	455:459	arg1	model					461:465	a mouse model	453:465	a mouse model	453:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	8	28	theme	adult	1435:1439	arg1	groups					1450:1455	young, adult, and old groups	1428:1455	young, adult, and old groups	1428:1455	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	2	29	theme	histological	340:351	arg1	alterations					383:393	the histological, molecular, and biomechanical alterations	336:393	the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model	336:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	2	30	from	enthesis	415:422	arg1	model					461:465	a mouse model	453:465	a mouse model	453:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	1	31	dep	interface	145:153	arg1	area					172:175	area	172:175	the tendon-to-bone interface (i.e., enthesis) area	126:175	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	1	32	theme	Rotator	82:88	arg1	injuries					102:109	Rotator cuff tendon injuries	82:109	Rotator cuff tendon injuries	82:109	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	7	33	theme	failure	1236:1242	arg1	stress					1244:1249	a significantly higher failure stress	1213:1249	a significantly higher failure stress when compared with the young and adult groups	1213:1295	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	4	34	theme	collagen	650:657	arg1	dispersion					708:717	collagen fiber dispersion	693:717	collagen fiber dispersion	693:717	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	4	34	theme	collagen	650:657	arg1	content					659:665	collagen content	650:665	collagen content	650:665	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	4	34	theme	collagen	650:657	arg1	deposition					681:690	proteoglycan deposition	668:690	proteoglycan deposition	668:690	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	1	35	dep	enthesis	162:169	arg1	i.e.					156:159	i.e.	156:159	i.e.	156:159	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	1	36	theme	tendon	95:100	arg1	injuries					102:109	Rotator cuff tendon injuries	82:109	Rotator cuff tendon injuries	82:109	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	8	37	theme	proteomics	1302:1311	arg1	analysis					1313:1320	The proteomics analysis	1298:1320	The proteomics analysis	1298:1320	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	2	38	theme	molecular	354:362	arg1	alterations					383:393	the histological, molecular, and biomechanical alterations	336:393	the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model	336:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	5	39	theme	thinner	932:938	arg1	fibers					949:954	thinner collagen fibers	932:954	thinner collagen fibers	932:954	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	2	40	theme	cuff	410:413	arg1	enthesis					415:422	the rotator cuff enthesis	398:422	the rotator cuff enthesis with maturation and aging in a mouse model	398:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	0	41	theme	rotator	43:49	arg1	tendon-to-bone					56:69	the rotator cuff tendon-to-bone	39:69	the rotator cuff tendon-to-bone	39:69	The effects of maturation and aging on the rotator cuff tendon-to-bone interface.					
34822203	7	42	theme	old	1190:1192	arg1	group					1194:1198	the old group	1186:1198	the old group	1186:1198	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	9	43	theme	old	1715:1717	arg1	age					1719:1721	old age	1715:1721	old age	1715:1721	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	4	44	theme	collagen	693:700	arg1	dispersion					708:717	collagen fiber dispersion	693:717	collagen fiber dispersion	693:717	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	4	44	theme	collagen	693:700	arg1	content					659:665	collagen content	650:665	collagen content	650:665	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	1	45	theme	high	185:188	arg1	prevalence					190:199	a high prevalence	183:199	a high prevalence for the elderly population	183:226	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	5	46	theme	increased	894:902	arg1	deposition					917:926	increased proteoglycan deposition	894:926	increased proteoglycan deposition	894:926	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	1	47	theme	tendon-to-bone	130:143	arg1	enthesis					162:169	enthesis	162:169	enthesis	162:169	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	1	47	theme	tendon-to-bone	130:143	arg1	interface					145:153	the tendon-to-bone interface	126:153	the tendon-to-bone interface (i.e., enthesis) area	126:175	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	8	48	theme	protein	1355:1361	arg1	content					1363:1369	protein content	1355:1369	protein content	1355:1369	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	9	49	theme	composition	1534:1544	arg1	alterations					1506:1516	the significant alterations	1490:1516	the significant alterations of the enthesis composition and structure	1490:1558	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	1	50	theme	elderly	209:215	arg1	population					217:226	the elderly population	205:226	the elderly population	205:226	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	9	51	theme	structure	1550:1558	arg1	alterations					1506:1516	the significant alterations	1490:1516	the significant alterations of the enthesis composition and structure	1490:1558	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	7	52	theme	tensile	1145:1151	arg1	testing					1164:1170	tensile mechanical testing	1145:1170	tensile mechanical testing	1145:1170	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	9	53	theme	significant	1494:1504	arg1	alterations					1506:1516	the significant alterations	1490:1516	the significant alterations of the enthesis composition and structure	1490:1558	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	3	54	theme	age	483:485	arg1	mice					497:500	mice	497:500	mice (newborn, young, adult, and old)	497:533	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	3	54	theme	age	483:485	arg1	groups					487:492	Four different age groups	468:492	Four different age groups of mice (newborn, young, adult, and old)	468:533	Four different age groups of mice (newborn, young, adult, and old) were studied.					
34822203	6	55	theme	smaller	1053:1059	arg1	modulus					1073:1079	a smaller compressive modulus	1051:1079	a smaller compressive modulus	1051:1079	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	9	56	theme	time	1596:1599	arg1	period					1601:1606	the young time period	1586:1606	the young time period	1586:1606	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	9	57	from	age	1719:1721	arg1	model					1743:1747	the rodent model	1732:1747	the rodent model	1732:1747	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	6	58	theme	old	1037:1039	arg1	group					1041:1045	the old group	1033:1045	the old group	1033:1045	Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups.					
34822203	5	59	theme	adult	820:824	arg1	groups					835:840	young, adult, and old groups	813:840	young, adult, and old groups	813:840	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	7	60	theme	adult	1284:1288	arg1	groups					1290:1295	the young and adult groups	1270:1295	the young and adult groups	1270:1295	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	4	61	theme	newborn	611:617	arg1	groups					637:642	the newborn and other matured groups	607:642	the newborn and other matured groups	607:642	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	8	62	theme	minor	1408:1412	arg1	changes					1414:1420	minor changes	1408:1420	minor changes	1408:1420	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	7	63	theme	young	1274:1278	arg1	groups					1290:1295	the young and adult groups	1270:1295	the young and adult groups	1270:1295	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	4	64	theme	other	623:627	arg1	groups					637:642	the newborn and other matured groups	607:642	the newborn and other matured groups	607:642	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	0	65	theme	cuff	51:54	arg1	tendon-to-bone					56:69	the rotator cuff tendon-to-bone	39:69	the rotator cuff tendon-to-bone	39:69	The effects of maturation and aging on the rotator cuff tendon-to-bone interface.					
34822203	2	66	from	alterations	383:393	arg1	model					461:465	a mouse model	453:465	a mouse model	453:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	2	67	with	enthesis	415:422	arg1	aging					444:448	aging	444:448	aging	444:448	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	2	67	with	enthesis	415:422	arg1	maturation					429:438	maturation	429:438	maturation	429:438	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	5	68	theme	young	813:817	arg1	groups					835:840	young, adult, and old groups	813:840	young, adult, and old groups	813:840	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	9	69	theme	rotator	1635:1641	arg1	injuries					1655:1662	rotator cuff tendon injuries	1635:1662	rotator cuff tendon injuries	1635:1662	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	0	70	from	effects	4:10	arg1	tendon-to-bone					56:69	the rotator cuff tendon-to-bone	39:69	the rotator cuff tendon-to-bone	39:69	The effects of maturation and aging on the rotator cuff tendon-to-bone interface.					
34822203	5	71	theme	significant	855:865	arg1	differences					867:877	significant differences	855:877	significant differences	855:877	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	9	72	theme	tendon	1648:1653	arg1	injuries					1655:1662	rotator cuff tendon injuries	1635:1662	rotator cuff tendon injuries	1635:1662	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	9	73	dep	age	1719:1721	arg1	2					1610:1610	2	1610:1610	2	1610:1610	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	4	74	dep	observed	590:597	arg1	with					645:648	with	645:648	with	645:648	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	8	75	theme	old	1446:1448	arg1	groups					1450:1455	young, adult, and old groups	1428:1455	young, adult, and old groups	1428:1455	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	4	76	theme	proteoglycan	668:679	arg1	content					659:665	collagen content	650:665	collagen content	650:665	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	4	76	theme	proteoglycan	668:679	arg1	deposition					681:690	proteoglycan deposition	668:690	proteoglycan deposition	668:690	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	9	77	theme	injuries	1655:1662	arg1	risk					1627:1630	the increased risk	1613:1630	the increased risk of rotator cuff tendon injuries in the elderly population	1613:1688	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	9	78	dep	occur	1560:1564	arg1	1					1487:1487	1	1487:1487	1	1487:1487	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	5	79	theme	proteoglycan	904:915	arg1	deposition					917:926	increased proteoglycan deposition	894:926	increased proteoglycan deposition	894:926	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	5	80	theme	groups	835:840	arg1	features					801:808	The compositional and histological features	766:808	The compositional and histological features of young, adult, and old groups	766:840	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	7	81	theme	higher	1229:1234	arg1	stress					1244:1249	a significantly higher failure stress	1213:1249	a significantly higher failure stress when compared with the young and adult groups	1213:1295	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	9	82	theme	elderly	1671:1677	arg1	population					1679:1688	the elderly population	1667:1688	the elderly population	1667:1688	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	0	83	theme	maturation	15:24	arg1	effects					4:10	The effects	0:10	The effects of maturation and aging on the rotator cuff tendon-to-bone	0:69	The effects of maturation and aging on the rotator cuff tendon-to-bone interface.					
34822203	1	84	theme	underlying	237:246	arg1	reason					248:253	the underlying reason	233:253	the underlying reason for this phenomenon	233:273	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	1	84	theme	underlying	237:246	arg1	unknown					284:290	unknown	284:290	unknown	284:290	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	1	85	theme	cuff	90:93	arg1	injuries					102:109	Rotator cuff tendon injuries	82:109	Rotator cuff tendon injuries	82:109	Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown.					
34822203	2	86	theme	biomechanical	369:381	arg1	alterations					383:393	the histological, molecular, and biomechanical alterations	336:393	the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model	336:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	0	87	theme	aging	30:34	arg1	effects					4:10	The effects	0:10	The effects of maturation and aging on the rotator cuff tendon-to-bone	0:69	The effects of maturation and aging on the rotator cuff tendon-to-bone interface.					
34822203	2	88	theme	enthesis	415:422	arg1	alterations					383:393	the histological, molecular, and biomechanical alterations	336:393	the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model	336:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	5	89	theme	collagen	940:947	arg1	fibers					949:954	thinner collagen fibers	932:954	thinner collagen fibers	932:954	The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group.					
34822203	2	90	theme	rotator	402:408	arg1	enthesis					415:422	the rotator cuff enthesis	398:422	the rotator cuff enthesis with maturation and aging in a mouse model	398:465	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
34822203	8	91	theme	dramatic	1331:1338	arg1	differences					1340:1350	dramatic differences	1331:1350	dramatic differences in protein content between newborn and young groups but minor changes	1331:1420	The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups.					
34822203	9	92	from	risk	1627:1630	arg1	population					1679:1688	the elderly population	1667:1688	the elderly population	1667:1688	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	9	93	theme	enthesis	1525:1532	arg1	composition					1534:1544	the enthesis composition	1521:1544	the enthesis composition	1521:1544	These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.					
34822203	7	94	theme	mechanical	1153:1162	arg1	testing					1164:1170	tensile mechanical testing	1145:1170	tensile mechanical testing	1145:1170	However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups.					
34822203	4	95	theme	fiber	702:706	arg1	dispersion					708:717	collagen fiber dispersion	693:717	collagen fiber dispersion	693:717	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	4	95	theme	fiber	702:706	arg1	content					659:665	collagen content	650:665	collagen content	650:665	Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group.					
34822203	2	96	theme	study	315:319	arg1	objective					297:305	The objective	293:305	The objective of this study	293:319	The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model.					
32112830	0	0	theme	oxide/silver	104:115	arg1	healing					192:198	cutaneous wound healing	176:198	cutaneous wound healing	176:198	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	0	theme	oxide/silver	104:115	arg1	performance					160:170	Antimicrobial performance	146:170	Antimicrobial performance	146:170	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	0	theme	oxide/silver	104:115	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	8	1	theme	tetrazolium	1174:1184	arg1	assay					1194:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	8	1	theme	tetrazolium	1174:1184	arg1	MTT					1201:1203	MTT	1201:1203	MTT	1201:1203	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	10	2	theme	most	1505:1508	arg1	effect					1510:1515	the most effect	1501:1515	the most effect on wound healing	1501:1532	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	7	3	theme	electron	1020:1027	arg1	microscopy					1029:1038	scanning electron microscopy	1011:1038	scanning electron microscopy (SEM)	1011:1044	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	7	3	theme	electron	1020:1027	arg1	SEM					1041:1043	SEM	1041:1043	SEM	1041:1043	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	9	4	theme	Gram-positive	1330:1342	arg1	bacteria					1344:1351	the Gram-negative and Gram-positive bacteria	1308:1351	the Gram-negative and Gram-positive bacteria	1308:1351	The scaffolds were able to hinder both of the Gram-negative and Gram-positive bacteria through direct contact with them.					
32112830	10	5	dep	good	1609:1612	arg1	biomedical					1614:1623	biomedical	1614:1623	biomedical	1614:1623	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	0	6	theme	graphene	95:102	arg1	healing					192:198	cutaneous wound healing	176:198	cutaneous wound healing	176:198	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	6	theme	graphene	95:102	arg1	performance					160:170	Antimicrobial performance	146:170	Antimicrobial performance	146:170	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	6	theme	graphene	95:102	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	9	7	theme	Gram-negative	1312:1324	arg1	bacteria					1344:1351	the Gram-negative and Gram-positive bacteria	1308:1351	the Gram-negative and Gram-positive bacteria	1308:1351	The scaffolds were able to hinder both of the Gram-negative and Gram-positive bacteria through direct contact with them.					
32112830	10	8	from	potential	1625:1633	arg1	healing					1660:1666	wound healing	1654:1666	wound healing	1654:1666	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	8	9	theme	composite	1244:1252	arg1	nanofibers					1254:1263	the composite nanofibers	1240:1263	the composite nanofibers	1240:1263	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	2	10	theme	due	398:400	arg1	mats					393:396	the mats	389:396	the mats due to the strong antibacterial activity of rGO/Ag nanocomposites	389:462	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	10	11	theme	healing	1554:1560	arg1	rate					1562:1565	the healing rate	1550:1565	the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing	1550:1666	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	3	12	theme	oxide	594:598	arg1	sheets					606:611	the reduced graphene oxide (rGO) sheets	573:611	the reduced graphene oxide (rGO) sheets	573:611	In order to prevent the agglomeration of silver nanoparticles, AgNPs, the nanoparticles were decorated onto the reduced graphene oxide (rGO) sheets.					
32112830	4	13	theme	Hummer	685:690	arg1	method					692:697	the improved Hummer method	672:697	the improved Hummer method	672:697	Initially, Graphene oxide, briefly GO, was synthesized by the improved Hummer method.					
32112830	8	14	theme	bromide	1186:1192	arg1	assay					1194:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	8	14	theme	bromide	1186:1192	arg1	MTT					1201:1203	MTT	1201:1203	MTT	1201:1203	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	5	15	theme	graphene	732:739	arg1	oxide					741:745	reduced graphene oxide	724:745	reduced graphene oxide	724:745	Then, nanocomposites of reduced graphene oxide were decorated with Ag and were fabricated via a green and facile hydrothermal method.					
32112830	4	16	theme	Graphene	625:632	arg1	oxide					634:638	Graphene oxide	625:638	Graphene oxide	625:638	Initially, Graphene oxide, briefly GO, was synthesized by the improved Hummer method.					
32112830	4	16	theme	Graphene	625:632	arg1	GO					649:650	GO	649:650	GO	649:650	Initially, Graphene oxide, briefly GO, was synthesized by the improved Hummer method.					
32112830	10	17	theme	good	1609:1612	arg1	potential					1625:1633	the good biomedical potential	1605:1633	the good biomedical potential of nanomaterial in wound healing	1605:1666	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	7	18	theme	transmission	1106:1117	arg1	WVTR					1125:1128	WVTR	1125:1128	WVTR	1125:1128	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	7	18	theme	transmission	1106:1117	arg1	rate					1119:1122	water vapor transmission rate	1094:1122	water vapor transmission rate (WVTR)	1094:1129	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	2	19	theme	oxide/silver	332:343	arg1	nanocomposites					345:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	2	19	theme	oxide/silver	332:343	arg1	rGO/Ag					361:366	rGO/Ag	361:366	rGO/Ag	361:366	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	10	20	dep	In	1387:1388	arg1	vivo					1390:1393	vivo	1390:1393	vivo	1390:1393	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	2	21	theme	rGO/Ag	442:447	arg1	nanocomposites					449:462	rGO/Ag nanocomposites	442:462	rGO/Ag nanocomposites	442:462	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	5	22	theme	hydrothermal	813:824	arg1	method					826:831	a green and facile hydrothermal method	794:831	method	826:831	Then, nanocomposites of reduced graphene oxide were decorated with Ag and were fabricated via a green and facile hydrothermal method.					
32112830	10	23	theme	wound	1520:1524	arg1	healing					1526:1532	wound healing	1520:1532	wound healing	1520:1532	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	2	24	used	used	379:382	arg2	rGO/Ag					361:366	rGO/Ag	361:366	rGO/Ag	361:366	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	2	24	used	used	379:382	arg2	nanocomposites					345:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	0	25	dep	nanocomposites	117:130	arg1	healing					192:198	cutaneous wound healing	176:198	cutaneous wound healing	176:198	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	25	dep	nanocomposites	117:130	arg1	performance					160:170	Antimicrobial performance	146:170	Antimicrobial performance	146:170	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	25	dep	nanocomposites	117:130	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	10	26	theme	In	1387:1388	arg1	studies					1413:1419	In vivo histopathological studies	1387:1419	In vivo histopathological studies	1387:1419	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	4	27	theme	improved	676:683	arg1	method					692:697	the improved Hummer method	672:697	the improved Hummer method	672:697	Initially, Graphene oxide, briefly GO, was synthesized by the improved Hummer method.					
32112830	0	28	theme	biomedical	4:13	arg1	potential					15:23	The biomedical potential	0:23	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats	0:74	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	8	29	theme	-2,5-diphenyl	1160:1172	arg1	assay					1194:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	8	29	theme	-2,5-diphenyl	1160:1172	arg1	MTT					1201:1203	MTT	1201:1203	MTT	1201:1203	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	7	30	theme	water	1094:1098	arg1	WVTR					1125:1128	WVTR	1125:1128	WVTR	1125:1128	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	7	30	theme	water	1094:1098	arg1	rate					1119:1122	water vapor transmission rate	1094:1122	water vapor transmission rate (WVTR)	1094:1129	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	7	31	theme	obtained	970:977	arg1	scaffolds					979:987	The obtained scaffolds	966:987	The obtained scaffolds	966:987	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	3	32	theme	reduced	577:583	arg1	sheets					606:611	the reduced graphene oxide (rGO) sheets	573:611	the reduced graphene oxide (rGO) sheets	573:611	In order to prevent the agglomeration of silver nanoparticles, AgNPs, the nanoparticles were decorated onto the reduced graphene oxide (rGO) sheets.					
32112830	5	33	theme	oxide	741:745	arg1	nanocomposites					706:719	nanocomposites	706:719	nanocomposites of reduced graphene oxide	706:745	Then, nanocomposites of reduced graphene oxide were decorated with Ag and were fabricated via a green and facile hydrothermal method.					
32112830	5	33	theme	oxide	741:745	arg1	oxide					741:745	reduced graphene oxide	724:745	reduced graphene oxide	724:745	Then, nanocomposites of reduced graphene oxide were decorated with Ag and were fabricated via a green and facile hydrothermal method.					
32112830	7	34	theme	vapor	1100:1104	arg1	WVTR					1125:1128	WVTR	1125:1128	WVTR	1125:1128	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	7	34	theme	vapor	1100:1104	arg1	rate					1119:1122	water vapor transmission rate	1094:1122	water vapor transmission rate (WVTR)	1094:1129	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	9	35	with	contact	1368:1374	arg1	them					1381:1384	them	1381:1384	them	1381:1384	The scaffolds were able to hinder both of the Gram-negative and Gram-positive bacteria through direct contact with them.					
32112830	2	36	theme	antibacterial	416:428	arg1	activity					430:437	the strong antibacterial activity	405:437	the strong antibacterial activity of rGO/Ag nanocomposites	405:462	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	10	37	theme	nanomaterial	1638:1649	arg1	potential					1625:1633	the good biomedical potential	1605:1633	the good biomedical potential of nanomaterial in wound healing	1605:1666	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	10	38	theme	rGO/Ag	1462:1467	arg1	nanocomposites					1469:1482	rGO/Ag nanocomposites	1462:1482	rGO/Ag nanocomposites	1462:1482	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	5	39	theme	reduced	724:730	arg1	oxide					741:745	reduced graphene oxide	724:745	reduced graphene oxide	724:745	Then, nanocomposites of reduced graphene oxide were decorated with Ag and were fabricated via a green and facile hydrothermal method.					
32112830	3	40	theme	nanoparticles	513:525	arg1	agglomeration					489:501	the agglomeration	485:501	the agglomeration of silver nanoparticles	485:525	In order to prevent the agglomeration of silver nanoparticles, AgNPs, the nanoparticles were decorated onto the reduced graphene oxide (rGO) sheets.					
32112830	10	41	theme	artificial	1570:1579	arg1	wounds					1581:1586	artificial wounds	1570:1586	artificial wounds	1570:1586	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	0	42	theme	Antimicrobial	146:158	arg1	performance					160:170	Antimicrobial performance	146:170	Antimicrobial performance	146:170	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	42	theme	Antimicrobial	146:158	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	43	theme	acetate/polyurethane	38:57	arg1	mats					71:74	cellulose acetate/polyurethane nanofibrous mats	28:74	cellulose acetate/polyurethane nanofibrous mats	28:74	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	44	theme	cutaneous	176:184	arg1	healing					192:198	cutaneous wound healing	176:198	cutaneous wound healing	176:198	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	44	theme	cutaneous	176:184	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	3	45	theme	graphene	585:592	arg1	oxide					594:598	graphene oxide	585:598	the reduced graphene oxide (rGO) sheets	573:611	In order to prevent the agglomeration of silver nanoparticles, AgNPs, the nanoparticles were decorated onto the reduced graphene oxide (rGO) sheets.					
32112830	3	45	theme	graphene	585:592	arg1	rGO					601:603	rGO	601:603	rGO	601:603	In order to prevent the agglomeration of silver nanoparticles, AgNPs, the nanoparticles were decorated onto the reduced graphene oxide (rGO) sheets.					
32112830	1	46	theme	nanofibrous	216:226	arg1	scaffolds					228:236	nanofibrous scaffolds	216:236	nanofibrous scaffolds	216:236	In this study, nanofibrous scaffolds were prepared from polyurethane and cellulose acetate using electrospinning.					
32112830	0	47	theme	cellulose	28:36	arg1	mats					71:74	cellulose acetate/polyurethane nanofibrous mats	28:74	cellulose acetate/polyurethane nanofibrous mats	28:74	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	10	48	theme	wounds	1581:1586	arg1	rate					1562:1565	the healing rate	1550:1565	the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing	1550:1666	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	2	49	theme	nanocomposites	449:462	arg1	activity					430:437	the strong antibacterial activity	405:437	the strong antibacterial activity of rGO/Ag nanocomposites	405:462	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	0	50	theme	mats	71:74	arg1	potential					15:23	The biomedical potential	0:23	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats	0:74	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	10	51	from	effect	1510:1515	arg1	healing					1526:1532	wound healing	1520:1532	wound healing	1520:1532	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	7	52	theme	tensile	1062:1068	arg1	analysis					1070:1077	tensile analysis	1062:1077	tensile analysis	1062:1077	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	8	53	theme	3-[4,5-Dimethylthiazol-2-yl	1132:1158	arg1	assay					1194:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay	1132:1198	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	8	53	theme	3-[4,5-Dimethylthiazol-2-yl	1132:1158	arg1	MTT					1201:1203	MTT	1201:1203	MTT	1201:1203	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	7	54	theme	contact	1047:1053	arg1	angle					1055:1059	contact angle	1047:1059	contact angle	1047:1059	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	0	55	theme	nanofibrous	59:69	arg1	mats					71:74	cellulose acetate/polyurethane nanofibrous mats	28:74	cellulose acetate/polyurethane nanofibrous mats	28:74	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	56	theme	wound	186:190	arg1	healing					192:198	cutaneous wound healing	176:198	cutaneous wound healing	176:198	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	56	theme	wound	186:190	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	6	57	theme	rGO/Ag	870:875	arg1	them					913:916	them	913:916	them	913:916	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	57	theme	rGO/Ag	870:875	arg1	both					905:908	both	905:908	both	905:908	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	57	theme	rGO/Ag	870:875	arg1	curcumin					893:900	curcumin	893:900	curcumin	893:900	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	57	theme	rGO/Ag	870:875	arg1	nanocomposites					877:890	the scaffold containing rGO/Ag nanocomposites	846:890	the scaffold containing rGO/Ag nanocomposites	846:890	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	58	theme	containing	859:868	arg1	them					913:916	them	913:916	them	913:916	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	58	theme	containing	859:868	arg1	both					905:908	both	905:908	both	905:908	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	58	theme	containing	859:868	arg1	curcumin					893:900	curcumin	893:900	curcumin	893:900	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	58	theme	containing	859:868	arg1	nanocomposites					877:890	the scaffold containing rGO/Ag nanocomposites	846:890	the scaffold containing rGO/Ag nanocomposites	846:890	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	2	59	theme	Reduced	315:321	arg1	nanocomposites					345:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	2	59	theme	Reduced	315:321	arg1	rGO/Ag					361:366	rGO/Ag	361:366	rGO/Ag	361:366	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	2	60	theme	graphene	323:330	arg1	nanocomposites					345:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites	315:358	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	2	60	theme	graphene	323:330	arg1	rGO/Ag					361:366	rGO/Ag	361:366	rGO/Ag	361:366	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	6	61	theme	scaffold	850:857	arg1	them					913:916	them	913:916	them	913:916	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	61	theme	scaffold	850:857	arg1	both					905:908	both	905:908	both	905:908	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	61	theme	scaffold	850:857	arg1	curcumin					893:900	curcumin	893:900	curcumin	893:900	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	6	61	theme	scaffold	850:857	arg1	nanocomposites					877:890	the scaffold containing rGO/Ag nanocomposites	846:890	the scaffold containing rGO/Ag nanocomposites	846:890	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	8	62	theme	nanofibers	1254:1263	arg1	biocompatibility					1220:1235	the biocompatibility	1216:1235	the biocompatibility of the composite nanofibers	1216:1263	3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay, MTT, confirmed the biocompatibility of the composite nanofibers.					
32112830	0	63	theme	reduced	87:93	arg1	healing					192:198	cutaneous wound healing	176:198	cutaneous wound healing	176:198	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	63	theme	reduced	87:93	arg1	performance					160:170	Antimicrobial performance	146:170	Antimicrobial performance	146:170	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	0	63	theme	reduced	87:93	arg1	nanocomposites					117:130	reduced graphene oxide/silver nanocomposites	87:130	reduced graphene oxide/silver nanocomposites	87:130	The biomedical potential of cellulose acetate/polyurethane nanofibrous mats containing reduced graphene oxide/silver nanocomposites and curcumin: Antimicrobial performance and cutaneous wound healing.					
32112830	3	64	theme	silver	506:511	arg1	nanoparticles					513:525	silver nanoparticles	506:525	silver nanoparticles	506:525	In order to prevent the agglomeration of silver nanoparticles, AgNPs, the nanoparticles were decorated onto the reduced graphene oxide (rGO) sheets.					
32112830	10	65	theme	histopathological	1395:1411	arg1	studies					1413:1419	In vivo histopathological studies	1387:1419	In vivo histopathological studies	1387:1419	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	2	66	theme	strong	409:414	arg1	activity					430:437	the strong antibacterial activity	405:437	the strong antibacterial activity of rGO/Ag nanocomposites	405:462	Reduced graphene oxide/silver nanocomposites, rGO/Ag, were also used into the mats due to the strong antibacterial activity of rGO/Ag nanocomposites.					
32112830	10	67	contain	has	1497:1499	arg1	scaffold					1440:1447	the scaffold	1436:1447	the scaffold incorporated rGO/Ag nanocomposites and curcumin	1436:1495	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	10	67	contain	has	1497:1499	arg2	effect					1510:1515	the most effect	1501:1515	the most effect on wound healing	1501:1532	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	1	68	theme	cellulose	274:282	arg1	acetate					284:290	cellulose acetate	274:290	cellulose acetate	274:290	In this study, nanofibrous scaffolds were prepared from polyurethane and cellulose acetate using electrospinning.					
32112830	5	69	theme	facile	806:811	arg1	method					826:831	a green and facile hydrothermal method	794:831	method	826:831	Then, nanocomposites of reduced graphene oxide were decorated with Ag and were fabricated via a green and facile hydrothermal method.					
32112830	6	70	theme	electrospinning	942:956	arg1	method					958:963	the electrospinning method	938:963	the electrospinning method	938:963	Thereafter, the scaffold containing rGO/Ag nanocomposites, curcumin or both of them were prepared using the electrospinning method.					
32112830	10	71	theme	wound	1654:1658	arg1	healing					1660:1666	wound healing	1654:1666	wound healing	1654:1666	In vivo histopathological studies indicated that the scaffold incorporated rGO/Ag nanocomposites and curcumin has the most effect on wound healing and can promote the healing rate of artificial wounds, which indicates the good biomedical potential of nanomaterial in wound healing.					
32112830	7	72	theme	scanning	1011:1018	arg1	microscopy					1029:1038	scanning electron microscopy	1011:1038	scanning electron microscopy (SEM)	1011:1044	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	7	72	theme	scanning	1011:1018	arg1	SEM					1041:1043	SEM	1041:1043	SEM	1041:1043	The obtained scaffolds were characterized by scanning electron microscopy (SEM), contact angle, tensile analysis, porosity, and water vapor transmission rate (WVTR).					
32112830	9	73	theme	direct	1361:1366	arg1	contact					1368:1374	direct contact	1361:1374	direct contact with them	1361:1384	The scaffolds were able to hinder both of the Gram-negative and Gram-positive bacteria through direct contact with them.					
34664584	8	0	theme	immune	1491:1496	arg1	response					1498:1505	immune response	1491:1505	immune response	1491:1505	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	4	1	theme	DSS-induced	714:724	arg1	colitis					732:738	DSS-induced acute colitis	714:738	DSS-induced acute colitis mice	714:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	1	2	theme	disease	275:281	arg1	development					241:251	development	241:251	development	241:251	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	1	2	theme	disease	275:281	arg1	occurrence					226:235	occurrence	226:235	occurrence	226:235	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	5	3	theme	acute	791:795	arg1	colitis					797:803	acute colitis	791:803	acute colitis mice	791:808	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	5	4	theme	colitis	797:803	arg1	mice					805:808	acute colitis mice	791:808	acute colitis mice	791:808	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	7	5	dep	FOS	1160:1162	arg1	treatment					1177:1185	treatment	1177:1185	treatment	1177:1185	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	1	6	theme	gut	185:187	arg1	microbiota					189:198	gut microbiota	185:198	gut microbiota	185:198	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	4	7	from	damage	704:709	arg1	mice					740:743	DSS-induced acute colitis mice	714:743	DSS-induced acute colitis mice	714:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	6	8	theme	tight	1012:1016	arg1	proteins					1027:1034	tight junction proteins	1012:1034	tight junction proteins	1012:1034	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	0	9	theme	barrier	125:131	arg1	impairment					100:109	the impairment	96:109	the impairment of intestinal barrier in DSS-induced acute colitis mice	96:165	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	7	10	theme	cytokines	1116:1124	arg1	expression					1085:1094	The expression	1081:1094	The expression of pro-inflammatory cytokines (IL-6 and TNF-α)	1081:1141	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	3	11	theme	dextran	559:565	arg1	DSS					583:585	DSS	583:585	DSS	583:585	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	3	11	theme	dextran	559:565	arg1	sodium					575:580	dextran sulfate sodium	559:580	dextran sulfate sodium (DSS)	559:586	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	4	12	theme	loss	676:679	arg1	damage					704:709	the body weight loss and colon histological damage	660:709	the body weight loss and colon histological damage in DSS-induced acute colitis mice	660:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	6	13	theme	epithelium	1001:1010	arg1	loss					968:971	DSS-induced loss	956:971	DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa	956:1078	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	0	14	theme	intestinal	114:123	arg1	barrier					125:131	intestinal barrier	114:131	intestinal barrier	114:131	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	8	15	theme	colitis	1528:1534	arg1	mice					1536:1539	DSS-induced acute colitis mice	1510:1539	DSS-induced acute colitis mice	1510:1539	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	8	16	theme	synbiotic	1313:1321	arg1	supplementation					1323:1337	synbiotic supplementation	1313:1337	synbiotic supplementation	1313:1337	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	3	17	theme	sulfate	567:573	arg1	DSS					583:585	DSS	583:585	DSS	583:585	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	3	17	theme	sulfate	567:573	arg1	sodium					575:580	dextran sulfate sodium	559:580	dextran sulfate sodium (DSS)	559:586	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	0	18	theme	DSS-induced	136:146	arg1	colitis					154:160	DSS-induced acute colitis	136:160	DSS-induced acute colitis mice	136:165	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	8	19	theme	acute	1522:1526	arg1	colitis					1528:1534	DSS-induced acute colitis	1510:1534	DSS-induced acute colitis mice	1510:1539	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	5	20	theme	decreased	885:893	arg1	abundance					895:903	decreased abundance	885:903	decreased abundance of Mucispirillum	885:920	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	1	21	theme	microbiota	189:198	arg1	dysbiosis					172:180	The dysbiosis	168:180	The dysbiosis of gut microbiota	168:198	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	1	21	theme	microbiota	189:198	arg1	related					211:217	related	211:217	related	211:217	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	3	22	theme	6-week-old	426:435	arg1	mice					445:448	6-week-old C57BL/J mice	426:448	6-week-old C57BL/J mice	426:448	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	8	23	theme	DSS-induced	1510:1520	arg1	colitis					1528:1534	DSS-induced acute colitis	1510:1534	DSS-induced acute colitis mice	1510:1539	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	4	24	theme	weight	669:674	arg1	loss					676:679	body weight loss	664:679	body weight loss	664:679	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	7	25	dep	cytokines	1116:1124	arg1	TNF-α					1136:1140	TNF-α	1136:1140	TNF-α	1136:1140	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	7	25	dep	cytokines	1116:1124	arg1	IL-6					1127:1130	IL-6	1127:1130	IL-6	1127:1130	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	7	25	dep	cytokines	1116:1124	arg1	cytokines					1116:1124	pro-inflammatory cytokines	1099:1124	pro-inflammatory cytokines (IL-6 and TNF-α)	1099:1141	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	6	26	theme	protein	982:988	arg1	loss					968:971	DSS-induced loss	956:971	DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa	956:1078	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	7	27	theme	Tbx21	1212:1216	arg1	expression					1198:1207	the expression	1194:1207	the expression of Tbx21 and IL-10	1194:1226	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	8	28	theme	inflammation	1358:1369	arg1	potential					1371:1379	the inflammation potential	1354:1379	the inflammation potential	1354:1379	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	6	29	theme	colon	1067:1071	arg1	mucosa					1073:1078	colon mucosa	1067:1078	colon mucosa	1067:1078	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	4	30	theme	colon	685:689	arg1	damage					704:709	the body weight loss and colon histological damage	660:709	the body weight loss and colon histological damage in DSS-induced acute colitis mice	660:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	0	31	theme	Fructooligosaccharide	0:20	arg1	supplementation					22:36	Fructooligosaccharide supplementation	0:36	Fructooligosaccharide supplementation	0:36	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	6	32	theme	junction	1018:1025	arg1	proteins					1027:1034	tight junction proteins	1012:1034	tight junction proteins	1012:1034	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	5	33	theme	abundance	895:903	arg1	characteristic					867:880	a characteristic	865:880	a characteristic of decreased abundance of Mucispirillum	865:920	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	0	34	theme	colitis	154:160	arg1	mice					162:165	DSS-induced acute colitis mice	136:165	DSS-induced acute colitis mice	136:165	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	2	35	theme	flora	321:325	arg1	manipulation					294:305	The manipulation	290:305	The manipulation of intestinal flora through prebiotics or probiotics	290:358	The manipulation of intestinal flora through prebiotics or probiotics is expected to induce and maintain the remission of IBD symptoms.					
34664584	8	36	theme	microbiota	1292:1301	arg1	modulation					1274:1283	the modulation	1270:1283	the modulation of gut microbiota by FOS or synbiotic supplementation	1270:1337	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	5	37	theme	Synbiotic	833:841	arg1	supplementation					843:857	Synbiotic supplementation	833:857	Synbiotic supplementation	833:857	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	0	38	theme	acute	148:152	arg1	colitis					154:160	DSS-induced acute colitis	136:160	DSS-induced acute colitis mice	136:165	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	5	39	from	composition	758:768	arg1	mice					805:808	acute colitis mice	791:808	acute colitis mice	791:808	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	2	40	theme	intestinal	310:319	arg1	flora					321:325	intestinal flora	310:325	intestinal flora	310:325	The manipulation of intestinal flora through prebiotics or probiotics is expected to induce and maintain the remission of IBD symptoms.					
34664584	0	41	theme	immune	66:71	arg1	response					73:80	the pathological immune response	49:80	the pathological immune response	49:80	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	4	42	theme	acute	726:730	arg1	colitis					732:738	DSS-induced acute colitis	714:738	DSS-induced acute colitis mice	714:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	0	43	from	impairment	100:109	arg1	mice					162:165	DSS-induced acute colitis mice	136:165	DSS-induced acute colitis mice	136:165	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	8	44	theme	commensals	1394:1403	arg1	potential					1371:1379	the inflammation potential	1354:1379	the inflammation potential	1354:1379	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	4	45	theme	body	664:667	arg1	loss					676:679	body weight loss	664:679	body weight loss	664:679	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	2	46	theme	IBD	412:414	arg1	symptoms					416:423	IBD symptoms	412:423	IBD symptoms	412:423	The manipulation of intestinal flora through prebiotics or probiotics is expected to induce and maintain the remission of IBD symptoms.					
34664584	6	47	theme	mucus	976:980	arg1	MUC2					991:994	MUC2	991:994	MUC2	991:994	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	6	47	theme	mucus	976:980	arg1	protein					982:988	mucus protein	976:988	mucus protein (MUC2)	976:995	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	0	48	theme	pathological	53:64	arg1	response					73:80	the pathological immune response	49:80	the pathological immune response	49:80	Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice.					
34664584	5	49	theme	altered	750:756	arg1	composition					758:768	The altered composition	746:768	The altered composition of gut microbiota in acute colitis mice	746:808	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	8	50	theme	colonized	1384:1392	arg1	commensals					1394:1403	colonized commensals	1384:1403	colonized commensals	1384:1403	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	2	51	theme	symptoms	416:423	arg1	remission					399:407	the remission	395:407	the remission of IBD symptoms	395:423	The manipulation of intestinal flora through prebiotics or probiotics is expected to induce and maintain the remission of IBD symptoms.					
34664584	6	52	from	loss	968:971	arg1	mucosa					1073:1078	colon mucosa	1067:1078	colon mucosa	1067:1078	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	8	53	theme	barrier	1455:1461	arg1	impairment					1426:1435	the impairment	1422:1435	the impairment of the intestinal barrier	1422:1461	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	4	54	theme	colitis	732:738	arg1	mice					740:743	DSS-induced acute colitis mice	714:743	DSS-induced acute colitis mice	714:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	8	55	theme	response	1498:1505	arg1	regulation					1477:1486	a regulation	1475:1486	a regulation of immune response in DSS-induced acute colitis mice	1475:1539	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	7	56	theme	pro-inflammatory	1099:1114	arg1	TNF-α					1136:1140	TNF-α	1136:1140	TNF-α	1136:1140	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	7	56	theme	pro-inflammatory	1099:1114	arg1	IL-6					1127:1130	IL-6	1127:1130	IL-6	1127:1130	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	7	56	theme	pro-inflammatory	1099:1114	arg1	cytokines					1116:1124	pro-inflammatory cytokines	1099:1124	pro-inflammatory cytokines (IL-6 and TNF-α)	1099:1141	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	3	57	theme	synbiotic	510:518	arg1	weeks					524:528	the synbiotic two weeks	506:528	the synbiotic two weeks before the administration of dextran sulfate sodium (DSS)	506:586	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	6	58	theme	DSS-induced	956:966	arg1	loss					968:971	DSS-induced loss	956:971	DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa	956:1078	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	6	59	dep	protein	982:988	arg1	Claudin1					1054:1061	Claudin1	1054:1061	Claudin1	1054:1061	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	6	59	dep	protein	982:988	arg1	Occluding					1043:1051	Occluding	1043:1051	Occluding	1043:1051	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	6	59	dep	protein	982:988	arg1	ZO-1					1037:1040	ZO-1	1037:1040	ZO-1	1037:1040	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	6	59	dep	protein	982:988	arg1	proteins					1027:1034	tight junction proteins	1012:1034	tight junction proteins	1012:1034	Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa.					
34664584	1	60	dep	occurrence	226:235	arg1	the					222:224	the	222:224	the	222:224	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	8	61	theme	gut	1288:1290	arg1	microbiota					1292:1301	gut microbiota	1288:1301	gut microbiota	1288:1301	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	3	62	theme	sodium	575:580	arg1	administration					541:554	the administration	537:554	the administration of dextran sulfate sodium (DSS)	537:586	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	8	63	theme	intestinal	1444:1453	arg1	barrier					1455:1461	the intestinal barrier	1440:1461	the intestinal barrier	1440:1461	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	5	64	theme	Mucispirillum	908:920	arg1	abundance					895:903	decreased abundance	885:903	decreased abundance of Mucispirillum	885:920	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	8	65	from	regulation	1477:1486	arg1	mice					1536:1539	DSS-induced acute colitis mice	1510:1539	DSS-induced acute colitis mice	1510:1539	The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.					
34664584	4	66	theme	FOS	612:614	arg1	supplementation					593:607	The supplementation	589:607	The supplementation of FOS or synbiotic	589:627	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	5	67	theme	gut	773:775	arg1	microbiota					777:786	gut microbiota	773:786	gut microbiota	773:786	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	1	68	theme	inflammatory	256:267	arg1	IBD					284:286	IBD	284:286	IBD	284:286	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	1	68	theme	inflammatory	256:267	arg1	disease					275:281	inflammatory bowel disease	256:281	inflammatory bowel disease (IBD)	256:287	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	5	69	theme	microbiota	777:786	arg1	composition					758:768	The altered composition	746:768	The altered composition of gut microbiota in acute colitis mice	746:808	The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum.					
34664584	3	70	theme	C57BL/J	437:443	arg1	mice					445:448	6-week-old C57BL/J mice	426:448	6-week-old C57BL/J mice	426:448	6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS).					
34664584	4	71	theme	synbiotic	619:627	arg1	supplementation					593:607	The supplementation	589:607	The supplementation of FOS or synbiotic	589:627	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	4	72	theme	histological	691:702	arg1	damage					704:709	the body weight loss and colon histological damage	660:709	the body weight loss and colon histological damage in DSS-induced acute colitis mice	660:743	The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice.					
34664584	7	73	theme	IL-10	1222:1226	arg1	expression					1198:1207	the expression	1194:1207	the expression of Tbx21 and IL-10	1194:1226	The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased.					
34664584	1	74	theme	bowel	269:273	arg1	IBD					284:286	IBD	284:286	IBD	284:286	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
34664584	1	74	theme	bowel	269:273	arg1	disease					275:281	inflammatory bowel disease	256:281	inflammatory bowel disease (IBD)	256:287	The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD).					
31955579	6	0	theme	motion	1011:1016	arg1	sensor					1018:1023	A skinlike motion sensor	1000:1023	A skinlike motion sensor with transparency	1000:1041	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	5	1	theme	nanoplates	988:997	arg1	addition					963:970	the addition	959:970	the addition of cationic LRH nanoplates	959:997	The multiple functions of the resultant hydrogel are easily realized by just relying on the addition of cationic LRH nanoplates.					
31955579	6	2	with	sensor	1018:1023	arg1	transparency					1030:1041	transparency	1030:1041	transparency	1030:1041	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	6	3	theme	skinlike	1002:1009	arg1	sensor					1018:1023	A skinlike motion sensor	1000:1023	A skinlike motion sensor with transparency	1000:1041	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	8	4	contain	have	1364:1367	arg2	potential					1369:1377	potential	1369:1377	potential for applications	1369:1394	This study reveals that the multifunctional hydrogels have potential for applications in sensing.					
31955579	8	4	contain	have	1364:1367	arg1	hydrogels					1354:1362	the multifunctional hydrogels	1334:1362	the multifunctional hydrogels	1334:1362	This study reveals that the multifunctional hydrogels have potential for applications in sensing.					
31955579	7	5	from	characteristics	1150:1164	arg1	LRH					1206:1208	LRH	1206:1208	LRH	1206:1208	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	0	6	theme	Motion	122:127	arg1	Sensing					129:135	Human Motion Sensing	116:135	Human Motion Sensing	116:135	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	2	7	theme	fluorescence	618:629	arg1	origin					608:613	the origin	604:613	the origin of fluorescence	604:629	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	0	8	theme	Human	116:120	arg1	Sensing					129:135	Human Motion Sensing	116:135	Human Motion Sensing	116:135	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	6	9	used	used	1094:1097	arg2	sensor					1018:1023	A skinlike motion sensor	1000:1023	A skinlike motion sensor with transparency	1000:1041	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	4	10	theme	electromechanical	841:857	arg1	properties					859:868	humidity-dependent electromechanical properties	822:868	humidity-dependent electromechanical properties	822:868	When exposed to different humidity conditions, the hydrogel exhibits humidity-dependent electromechanical properties.					
31955579	1	11	theme	soft	285:288	arg1	robotics					290:297	soft robotics	285:297	soft robotics	285:297	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	4	12	theme	humidity	779:786	arg1	conditions					788:797	different humidity conditions	769:797	different humidity conditions	769:797	When exposed to different humidity conditions, the hydrogel exhibits humidity-dependent electromechanical properties.					
31955579	7	13	theme	tunable	1237:1243	arg1	photoluminescence					1258:1274	highly tunable multicolored photoluminescence	1230:1274	highly tunable multicolored photoluminescence	1230:1274	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	5	14	theme	resultant	901:909	arg1	hydrogel					911:918	the resultant hydrogel	897:918	the resultant hydrogel	897:918	The multiple functions of the resultant hydrogel are easily realized by just relying on the addition of cationic LRH nanoplates.					
31955579	8	15	theme	multifunctional	1338:1352	arg1	hydrogels					1354:1362	the multifunctional hydrogels	1334:1362	the multifunctional hydrogels	1334:1362	This study reveals that the multifunctional hydrogels have potential for applications in sensing.					
31955579	2	16	theme	nanocomposite	413:425	arg1	hydrogel					427:434	a multifunctional nanocomposite hydrogel	395:434	a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence	395:629	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	5	17	theme	multiple	875:882	arg1	functions					884:892	The multiple functions	871:892	The multiple functions of the resultant hydrogel	871:918	The multiple functions of the resultant hydrogel are easily realized by just relying on the addition of cationic LRH nanoplates.					
31955579	0	18	theme	Skin-Inspired	0:12	arg1	Hydrogel					42:49	Skin-Inspired Multifunctional Luminescent Hydrogel	0:49	Skin-Inspired Multifunctional Luminescent Hydrogel	0:49	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	2	19	theme	multifunctional	397:411	arg1	hydrogel					427:434	a multifunctional nanocomposite hydrogel	395:434	a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence	395:629	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	0	20	theme	Multifunctional	14:28	arg1	Hydrogel					42:49	Skin-Inspired Multifunctional Luminescent Hydrogel	0:49	Skin-Inspired Multifunctional Luminescent Hydrogel	0:49	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	7	21	theme	ions	1180:1183	arg1	characteristics					1150:1164	the fluorescence characteristics	1133:1164	the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH	1133:1208	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	2	22	theme	multiple	538:545	arg1	carrier					584:590	a co-cross-linker and ionic carrier	556:590	a co-cross-linker and ionic carrier	556:590	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	22	theme	multiple	538:545	arg1	roles					547:551	multiple roles	538:551	multiple roles	538:551	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	23	theme	effective	318:326	arg1	strategy					360:367	an effective and facile one-step preparation strategy	315:367	an effective and facile one-step preparation strategy	315:367	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	7	24	theme	multicolored	1245:1256	arg1	photoluminescence					1258:1274	highly tunable multicolored photoluminescence	1230:1274	highly tunable multicolored photoluminescence	1230:1274	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	1	25	theme	attention	203:211	arg1	attention					203:211	attention	203:211	attention owing to its application in electronic skins, wearable electronics, and soft robotics	203:297	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	1	25	theme	attention	203:211	arg1	lot					196:198	a lot	194:198	a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics	194:297	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	2	26	theme	rare-earth	494:503	arg1	LRH					516:518	LRH	516:518	LRH	516:518	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	26	theme	rare-earth	494:503	arg1	hydroxide					505:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide	450:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH)	450:519	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	6	27	theme	printed	1070:1076	arg1	hydrogel					1078:1085	the printed hydrogel	1066:1085	the printed hydrogel	1066:1085	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	2	28	theme	polyacrylate/layered	473:492	arg1	LRH					516:518	LRH	516:518	LRH	516:518	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	28	theme	polyacrylate/layered	473:492	arg1	hydroxide					505:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide	450:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH)	450:519	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	5	29	theme	hydrogel	911:918	arg1	functions					884:892	The multiple functions	871:892	The multiple functions of the resultant hydrogel	871:918	The multiple functions of the resultant hydrogel are easily realized by just relying on the addition of cationic LRH nanoplates.					
31955579	7	30	dep	ions	1180:1183	arg1	Eu3+					1186:1189	Eu3+	1186:1189	Eu3+	1186:1189	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	7	30	dep	ions	1180:1183	arg1	Tb3+					1195:1198	Tb3+	1195:1198	Tb3+	1195:1198	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	4	31	theme	humidity-dependent	822:839	arg1	properties					859:868	humidity-dependent electromechanical properties	822:868	humidity-dependent electromechanical properties	822:868	When exposed to different humidity conditions, the hydrogel exhibits humidity-dependent electromechanical properties.					
31955579	2	32	theme	sodium	450:455	arg1	LRH					516:518	LRH	516:518	LRH	516:518	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	32	theme	sodium	450:455	arg1	hydroxide					505:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide	450:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH)	450:519	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	33	theme	alginate/sodium	457:471	arg1	LRH					516:518	LRH	516:518	LRH	516:518	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	33	theme	alginate/sodium	457:471	arg1	hydroxide					505:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide	450:513	sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH)	450:519	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	7	34	theme	fluorescence	1137:1148	arg1	characteristics					1150:1164	the fluorescence characteristics	1133:1164	the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH	1133:1208	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	7	35	theme	lanthanide	1169:1178	arg1	ions					1180:1183	lanthanide ions	1169:1183	lanthanide ions (Eu3+ and Tb3+) from LRH	1169:1208	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	3	36	theme	three-dimensional	693:709	arg1	performance					720:730	excellent three-dimensional printing performance	683:730	excellent three-dimensional printing performance	683:730	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	5	37	theme	cationic	975:982	arg1	nanoplates					988:997	cationic LRH nanoplates	975:997	cationic LRH nanoplates	975:997	The multiple functions of the resultant hydrogel are easily realized by just relying on the addition of cationic LRH nanoplates.					
31955579	0	38	theme	Layered	62:68	arg1	Hydroxide					81:89	Layered Rare-Earth Hydroxide	62:89	Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing	62:135	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	4	39	theme	different	769:777	arg1	conditions					788:797	different humidity conditions	769:797	different humidity conditions	769:797	When exposed to different humidity conditions, the hydrogel exhibits humidity-dependent electromechanical properties.					
31955579	2	40	theme	ionic	578:582	arg1	carrier					584:590	a co-cross-linker and ionic carrier	556:590	a co-cross-linker and ionic carrier	556:590	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	40	theme	ionic	578:582	arg1	roles					547:551	multiple roles	538:551	multiple roles	538:551	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	3	41	theme	printing	711:718	arg1	performance					720:730	excellent three-dimensional printing performance	683:730	excellent three-dimensional printing performance	683:730	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	6	42	dep	fabricated	1046:1055	arg1	based					1057:1061	based	1057:1061	is fabricated based on the printed hydrogel	1043:1085	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	1	43	from	application	226:236	arg1	robotics					290:297	soft robotics	285:297	soft robotics	285:297	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	1	43	from	application	226:236	arg1	skins					252:256	electronic skins	241:256	electronic skins	241:256	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	1	43	from	application	226:236	arg1	electronics					268:278	wearable electronics	259:278	wearable electronics	259:278	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	7	44	from	LRH	1206:1208	arg1	characteristics					1150:1164	the fluorescence characteristics	1133:1164	the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH	1133:1208	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	7	44	from	LRH	1206:1208	arg1	ions					1180:1183	lanthanide ions	1169:1183	lanthanide ions (Eu3+ and Tb3+) from LRH	1169:1208	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	2	45	theme	co-cross-linker	558:572	arg1	carrier					584:590	a co-cross-linker and ionic carrier	556:590	a co-cross-linker and ionic carrier	556:590	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	2	45	theme	co-cross-linker	558:572	arg1	roles					547:551	multiple roles	538:551	multiple roles	538:551	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	3	46	theme	excellent	683:691	arg1	performance					720:730	excellent three-dimensional printing performance	683:730	excellent three-dimensional printing performance	683:730	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	2	47	theme	preparation	348:358	arg1	strategy					360:367	an effective and facile one-step preparation strategy	315:367	an effective and facile one-step preparation strategy	315:367	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	0	48	theme	Rare-Earth	70:79	arg1	Hydroxide					81:89	Layered Rare-Earth Hydroxide	62:89	Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing	62:135	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	3	49	theme	room	735:738	arg1	temperature					740:750	room temperature	735:750	room temperature	735:750	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	7	50	theme	LRH	1293:1295	arg1	constituent					1297:1307	the LRH constituent	1289:1307	the LRH constituent	1289:1307	Owing to the fluorescence characteristics of lanthanide ions (Eu3+ and Tb3+) from LRH, the hydrogel shows highly tunable multicolored photoluminescence by adjusting the LRH constituent.					
31955579	2	51	theme	one-step	339:346	arg1	strategy					360:367	an effective and facile one-step preparation strategy	315:367	an effective and facile one-step preparation strategy	315:367	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	3	52	theme	LRH-based	645:653	arg1	hydrogel					665:672	The obtained LRH-based composite hydrogel	632:672	The obtained LRH-based composite hydrogel	632:672	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	3	53	theme	composite	655:663	arg1	hydrogel					665:672	The obtained LRH-based composite hydrogel	632:672	The obtained LRH-based composite hydrogel	632:672	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	2	54	theme	facile	332:337	arg1	strategy					360:367	an effective and facile one-step preparation strategy	315:367	an effective and facile one-step preparation strategy	315:367	In this study, an effective and facile one-step preparation strategy is developed to fabricate a multifunctional nanocomposite hydrogel consisting of sodium alginate/sodium polyacrylate/layered rare-earth hydroxide (LRH), where LRH plays multiple roles as a co-cross-linker and ionic carrier and is also the origin of fluorescence.					
31955579	1	55	theme	electronic	241:250	arg1	skins					252:256	electronic skins	241:256	electronic skins	241:256	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	0	56	with	Hydroxide	81:89	arg1	Printability					99:110	3D Printability	96:110	3D Printability	96:110	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	1	57	theme	multifunctional	157:171	arg1	hydrogels					173:181	multifunctional hydrogels	157:181	multifunctional hydrogels	157:181	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	6	58	theme	human	1110:1114	arg1	motion					1116:1121	human motion	1110:1121	human motion	1110:1121	A skinlike motion sensor with transparency is fabricated based on the printed hydrogel and is used to monitor human motion.					
31955579	0	59	theme	3D	96:97	arg1	Printability					99:110	3D Printability	96:110	3D Printability	96:110	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	3	60	theme	obtained	636:643	arg1	hydrogel					665:672	The obtained LRH-based composite hydrogel	632:672	The obtained LRH-based composite hydrogel	632:672	The obtained LRH-based composite hydrogel exhibits excellent three-dimensional printing performance at room temperature.					
31955579	1	61	theme	hydrogels	173:181	arg1	development					142:152	The development	138:152	The development of multifunctional hydrogels	138:181	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
31955579	0	62	theme	Luminescent	30:40	arg1	Hydrogel					42:49	Skin-Inspired Multifunctional Luminescent Hydrogel	0:49	Skin-Inspired Multifunctional Luminescent Hydrogel	0:49	Skin-Inspired Multifunctional Luminescent Hydrogel Containing Layered Rare-Earth Hydroxide with 3D Printability for Human Motion Sensing.					
31955579	5	63	theme	LRH	984:986	arg1	nanoplates					988:997	cationic LRH nanoplates	975:997	cationic LRH nanoplates	975:997	The multiple functions of the resultant hydrogel are easily realized by just relying on the addition of cationic LRH nanoplates.					
31955579	1	64	theme	wearable	259:266	arg1	electronics					268:278	wearable electronics	259:278	wearable electronics	259:278	The development of multifunctional hydrogels is gaining a lot of attention owing to its application in electronic skins, wearable electronics, and soft robotics.					
33988217	3	0	from	pH	662:663	arg1	charged					651:657	charged	651:657	charged	651:657	A total of 414 sequences were obtained with 56 duplicates, half of which were positively charged at pH 7.					
33988217	8	1	theme	storage	1295:1301	arg1	G					1312:1312	G'	1312:1313	G'	1312:1313	The values of the storage modulus (G') of 11 dipeptides/ι-C were investigated by a rheometer.					
33988217	8	1	theme	storage	1295:1301	arg1	modulus					1303:1309	the storage modulus	1291:1309	the storage modulus (G') of 11 dipeptides/ι-C	1291:1335	The values of the storage modulus (G') of 11 dipeptides/ι-C were investigated by a rheometer.					
33988217	4	2	theme	cation	678:683	arg1	sequences					685:693	the cation sequences	674:693	the cation sequences	674:693	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	10	3	dep	design	1556:1561	arg1	the					1552:1554	the	1552:1554	the	1552:1554	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	10	4	theme	hydrogels	1593:1601	arg1	development					1567:1577	development	1567:1577	development	1567:1577	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	10	4	theme	hydrogels	1593:1601	arg1	design					1556:1561	design	1556:1561	design	1556:1561	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	10	5	theme	silico-screened	1459:1473	arg1	dipeptides					1475:1484	the in silico-screened dipeptides	1452:1484	the in silico-screened dipeptides from SMGs	1452:1494	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	6	6	theme	ι-C	951:953	arg1	effect					933:938	the obvious synergistic effect	909:938	the obvious synergistic effect of Lys and ι-C	909:953	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	10	7	theme	functional	1582:1591	arg1	hydrogels					1593:1601	functional hydrogels	1582:1601	functional hydrogels	1582:1601	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	10	8	theme	composite	1505:1513	arg1	gels					1515:1518	composite gels	1505:1518	composite gels	1505:1518	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	6	9	dep	Ser-Lys	996:1002	arg1	SK					1005:1006	SK	1005:1006	SK	1005:1006	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	10	10	from	SMGs	1491:1494	arg1	dipeptides					1475:1484	the in silico-screened dipeptides	1452:1484	the in silico-screened dipeptides from SMGs	1452:1494	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	1	11	dep	in	279:280	arg1	silico					282:287	silico	282:287	silico	282:287	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	6	12	theme	Lys	943:945	arg1	effect					933:938	the obvious synergistic effect	909:938	the obvious synergistic effect of Lys and ι-C	909:953	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	13	with	gelation	1189:1196	arg1	ι-C					1203:1205	ι-C	1203:1205	ι-C	1203:1205	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	10	14	theme	in	1456:1457	arg1	dipeptides					1475:1484	the in silico-screened dipeptides	1452:1484	the in silico-screened dipeptides from SMGs	1452:1494	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
33988217	3	15	theme	414	573:575	arg1	sequences					577:585	414 sequences	573:585	414 sequences	573:585	A total of 414 sequences were obtained with 56 duplicates, half of which were positively charged at pH 7.					
33988217	4	16	contain	had	700:702	arg1	171					696:698	171	696:698	171	696:698	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	4	16	contain	had	700:702	arg2	solubility					715:724	good water solubility	704:724	good water solubility	704:724	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	5	17	theme	sequences	828:836	arg1	analysis					789:796	The molecular weight analysis	768:796	The molecular weight analysis of the cationic water-soluble sequences	768:836	The molecular weight analysis of the cationic water-soluble sequences showed that 0.2-0.3 kDa accounted for the highest proportion.					
33988217	5	18	theme	cationic	805:812	arg1	sequences					828:836	the cationic water-soluble sequences	801:836	the cationic water-soluble sequences	801:836	The molecular weight analysis of the cationic water-soluble sequences showed that 0.2-0.3 kDa accounted for the highest proportion.					
33988217	2	19	theme	liquid	429:434	arg1	nanoLC-MS/MS					488:499	nanoLC-MS/MS	488:499	nanoLC-MS/MS	488:499	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	19	theme	liquid	429:434	arg1	spectrometry					474:485	nano liquid chromatography-mass spectrometry/mass spectrometry	424:485	nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS)	424:500	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	0	20	from	scallop	44:50	arg1	dipeptides					28:37	silico-screened cationic dipeptides	3:37	silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan	3:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	4	21	theme	amino	741:745	arg1	Arg					762:764	Arg	762:764	Arg	762:764	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	4	21	theme	amino	741:745	arg1	Lys					754:756	Lys	754:756	Lys	754:756	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	4	21	theme	amino	741:745	arg1	acids					747:751	two amino acids	737:751	two amino acids (Lys and Arg)	737:765	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	5	22	theme	water-soluble	814:826	arg1	sequences					828:836	the cationic water-soluble sequences	801:836	the cationic water-soluble sequences	801:836	The molecular weight analysis of the cationic water-soluble sequences showed that 0.2-0.3 kDa accounted for the highest proportion.					
33988217	2	23	theme	nano	424:427	arg1	nanoLC-MS/MS					488:499	nanoLC-MS/MS	488:499	nanoLC-MS/MS	488:499	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	23	theme	nano	424:427	arg1	spectrometry					474:485	nano liquid chromatography-mass spectrometry/mass spectrometry	424:485	nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS)	424:500	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	4	24	theme	water	709:713	arg1	solubility					715:724	good water solubility	704:724	good water solubility	704:724	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	1	25	theme	male	182:185	arg1	SMGs					195:198	SMGs	195:198	SMGs	195:198	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	1	25	theme	male	182:185	arg1	gonads					187:192	scallop (Patinopecten yessoensis) male gonads	148:192	scallop (Patinopecten yessoensis) male gonads (SMGs)	148:199	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	1	25	theme	male	182:185	arg1	Patinopecten					157:168	Patinopecten	157:168	Patinopecten	157:168	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	9	26	theme	dipeptides/ι-C	1383:1396	arg1	G					1375:1375	The G	1371:1375	The G' of 8 dipeptides/ι-C	1371:1396	The G' of 8 dipeptides/ι-C was higher than 1000 Pa.					
33988217	9	26	theme	dipeptides/ι-C	1383:1396	arg1	higher					1402:1407	higher	1402:1407	higher	1402:1407	The G' of 8 dipeptides/ι-C was higher than 1000 Pa.					
33988217	1	27	dep	Patinopecten	157:168	arg1	yessoensis					170:179	Patinopecten yessoensis	157:179	Patinopecten yessoensis	157:179	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	4	28	theme	good	704:707	arg1	solubility					715:724	good water solubility	704:724	good water solubility	704:724	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	5	29	theme	weight	782:787	arg1	analysis					789:796	The molecular weight analysis	768:796	The molecular weight analysis of the cationic water-soluble sequences	768:836	The molecular weight analysis of the cationic water-soluble sequences showed that 0.2-0.3 kDa accounted for the highest proportion.					
33988217	1	30	from	gonads	187:192	arg1	dipeptides					132:141	some cationic dipeptides	118:141	some cationic dipeptides	118:141	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	6	31	theme	synergistic	921:931	arg1	effect					933:938	the obvious synergistic effect	909:938	the obvious synergistic effect of Lys and ι-C	909:953	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	2	32	theme	electrophoresis	393:407	arg1	analysis					502:509	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis	351:509	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis	351:509	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	33	theme	spectrometry	474:485	arg1	analysis					502:509	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis	351:509	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis	351:509	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	6	34	theme	obvious	913:919	arg1	effect					933:938	the obvious synergistic effect	909:938	the obvious synergistic effect of Lys and ι-C	909:953	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	35	theme	Ser-Lys	996:1002	arg1	TK					1019:1020	TK	1019:1020	TK	1019:1020	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	35	theme	Ser-Lys	996:1002	arg1	Thr-Lys					1010:1016	Thr-Lys	1010:1016	Thr-Lys (TK)	1010:1021	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	2	36	theme	gel	389:391	arg1	SDS-PAGE					410:417	SDS-PAGE	410:417	SDS-PAGE	410:417	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	36	theme	gel	389:391	arg1	electrophoresis					393:407	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	351:407	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	351:418	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	37	theme	spectrometry/mass	456:472	arg1	nanoLC-MS/MS					488:499	nanoLC-MS/MS	488:499	nanoLC-MS/MS	488:499	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	37	theme	spectrometry/mass	456:472	arg1	spectrometry					474:485	nano liquid chromatography-mass spectrometry/mass spectrometry	424:485	nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS)	424:500	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	0	38	from	effect	78:83	arg1	ι-carrageenan					88:100	ι-carrageenan	88:100	ι-carrageenan	88:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	8	39	theme	dipeptides/ι-C	1322:1335	arg1	G					1312:1312	G'	1312:1313	G'	1312:1313	The values of the storage modulus (G') of 11 dipeptides/ι-C were investigated by a rheometer.					
33988217	8	39	theme	dipeptides/ι-C	1322:1335	arg1	modulus					1303:1309	the storage modulus	1291:1309	the storage modulus (G') of 11 dipeptides/ι-C	1291:1335	The values of the storage modulus (G') of 11 dipeptides/ι-C were investigated by a rheometer.					
33988217	2	40	theme	sulfate-polyacrylamide	366:387	arg1	SDS-PAGE					410:417	SDS-PAGE	410:417	SDS-PAGE	410:417	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	40	theme	sulfate-polyacrylamide	366:387	arg1	electrophoresis					393:407	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	351:407	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	351:418	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	41	theme	chromatography-mass	436:454	arg1	nanoLC-MS/MS					488:499	nanoLC-MS/MS	488:499	nanoLC-MS/MS	488:499	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	41	theme	chromatography-mass	436:454	arg1	spectrometry					474:485	nano liquid chromatography-mass spectrometry/mass spectrometry	424:485	nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS)	424:500	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	8	42	theme	modulus	1303:1309	arg1	values					1281:1286	The values	1277:1286	The values of the storage modulus (G') of 11 dipeptides/ι-C	1277:1335	The values of the storage modulus (G') of 11 dipeptides/ι-C were investigated by a rheometer.					
33988217	0	43	theme	gelation	69:76	arg1	effect					78:83	synergistic gelation effect	57:83	synergistic gelation effect on ι-carrageenan	57:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	3	44	theme	sequences	577:585	arg1	total					564:568	A total	562:568	A total of 414 sequences	562:585	A total of 414 sequences were obtained with 56 duplicates, half of which were positively charged at pH 7.					
33988217	2	45	theme	protein	308:314	arg1	sequences					316:324	Fourteen protein sequences	299:324	Fourteen protein sequences of SMGs	299:332	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	2	46	theme	SMGs	329:332	arg1	sequences					316:324	Fourteen protein sequences	299:324	Fourteen protein sequences of SMGs	299:332	Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis and were then hydrolyzed via in silico simulation.					
33988217	1	47	theme	in	279:280	arg1	approach					289:296	the in silico approach	275:296	the in silico approach	275:296	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	7	48	theme	firm	1252:1255	arg1	gels					1257:1260	firm gels	1252:1260	firm gels	1252:1260	It was found that the dipeptides/ι-C formed firm gels except WK/ι-C.					
33988217	3	49	theme	duplicates	609:618	arg1	half					621:624	half	621:624	half	621:624	A total of 414 sequences were obtained with 56 duplicates, half of which were positively charged at pH 7.					
33988217	1	50	theme	cationic	123:130	arg1	dipeptides					132:141	some cationic dipeptides	118:141	some cationic dipeptides	118:141	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	5	51	theme	molecular	772:780	arg1	analysis					789:796	The molecular weight analysis	768:796	The molecular weight analysis of the cationic water-soluble sequences	768:836	The molecular weight analysis of the cationic water-soluble sequences showed that 0.2-0.3 kDa accounted for the highest proportion.					
33988217	1	52	theme	scallop	148:154	arg1	SMGs					195:198	SMGs	195:198	SMGs	195:198	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	1	52	theme	scallop	148:154	arg1	gonads					187:192	scallop (Patinopecten yessoensis) male gonads	148:192	scallop (Patinopecten yessoensis) male gonads (SMGs)	148:199	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	1	52	theme	scallop	148:154	arg1	Patinopecten					157:168	Patinopecten	157:168	Patinopecten	157:168	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	5	53	theme	highest	880:886	arg1	proportion					888:897	the highest proportion	876:897	the highest proportion	876:897	The molecular weight analysis of the cationic water-soluble sequences showed that 0.2-0.3 kDa accounted for the highest proportion.					
33988217	1	54	with	gel	228:230	arg1	ι-carrageenan					237:249	ι-carrageenan	237:249	ι-carrageenan (ι-C)	237:255	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	1	54	with	gel	228:230	arg1	ι-C					252:254	ι-C	252:254	ι-C	252:254	In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.					
33988217	4	55	dep	acids	747:751	arg1	Arg					762:764	Arg	762:764	Arg	762:764	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	4	55	dep	acids	747:751	arg1	Lys					754:756	Lys	754:756	Lys	754:756	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	4	55	dep	acids	747:751	arg1	acids					747:751	two amino acids	737:751	two amino acids (Lys and Arg)	737:765	Among the cation sequences, 171 had good water solubility, including two amino acids (Lys and Arg).					
33988217	6	56	theme	Lys-containing	959:972	arg1	Gly-Lys					1066:1072	Gly-Lys	1066:1072	Gly-Lys (GK)	1066:1077	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Phe-Lys					1122:1128	Phe-Lys	1122:1128	Phe-Lys (FK)	1122:1133	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Met-Lys					1140:1146	Met-Lys	1140:1146	Met-Lys (MK)	1140:1151	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Leu-Lys					1052:1058	Leu-Lys	1052:1058	Leu-Lys (LK)	1052:1063	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Asn-Lys					1108:1114	Asn-Lys	1108:1114	Asn-Lys (NK)	1108:1119	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Ala-Lys					1038:1044	Ala-Lys	1038:1044	Ala-Lys (AK)	1038:1049	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Trp-Lys					1024:1030	Trp-Lys	1024:1030	Trp-Lys (WK)	1024:1035	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	dipeptides					974:983	11 Lys-containing dipeptides	956:983	11 Lys-containing dipeptides	956:983	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Cys-Lys					1094:1100	Cys-Lys	1094:1100	Cys-Lys (CK)	1094:1105	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Val-Lys					1080:1086	Val-Lys	1080:1086	Val-Lys (VK)	1080:1091	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	6	56	theme	Lys-containing	959:972	arg1	Thr-Lys					1010:1016	Thr-Lys	1010:1016	Thr-Lys (TK)	1010:1021	Based on the obvious synergistic effect of Lys and ι-C, 11 Lys-containing dipeptides, including Ser-Lys (SK), Thr-Lys (TK), Trp-Lys (WK), Ala-Lys (AK), Leu-Lys (LK), Gly-Lys (GK), Val-Lys (VK), Cys-Lys (CK), Asn-Lys (NK), Phe-Lys (FK), and Met-Lys (MK), were finally screened out to study gelation with ι-C.					
33988217	0	57	theme	synergistic	57:67	arg1	effect					78:83	synergistic gelation effect	57:83	synergistic gelation effect on ι-carrageenan	57:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	0	58	theme	cationic	19:26	arg1	dipeptides					28:37	silico-screened cationic dipeptides	3:37	silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan	3:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	0	59	with	scallop	44:50	arg1	effect					78:83	synergistic gelation effect	57:83	synergistic gelation effect on ι-carrageenan	57:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	0	60	theme	silico-screened	3:17	arg1	dipeptides					28:37	silico-screened cationic dipeptides	3:37	silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan	3:100	In silico-screened cationic dipeptides from scallop with synergistic gelation effect on ι-carrageenan.					
33988217	10	61	used	used	1543:1546	arg2	ι-C					1525:1527	ι-C	1525:1527	ι-C	1525:1527	These results indicated that the in silico-screened dipeptides from SMGs can form composite gels with ι-C, which can be used for the design and development of functional hydrogels.					
32062390	7	0	theme	several	1081:1087	arg1	processes					1104:1112	several photocatalytic processes	1081:1112	several photocatalytic processes	1081:1112	The findings displayed the probability of reusing the catalyst material for several photocatalytic processes.					
32062390	11	1	theme	@	1450:1450	arg1	material					1470:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	1	theme	@	1450:1450	arg1	catalyst					1496:1503	a catalyst	1494:1503	a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions	1494:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	9	2	theme	degradation	1237:1247	arg1	results					1249:1255	The obtained MB degradation results	1221:1255	The obtained MB degradation results	1221:1255	The obtained MB degradation results were fitted onto a Langmuir-Hinshelwood (L-H) plot.					
32062390	11	3	used	used	1486:1489	arg2	catalyst					1496:1503	a catalyst	1494:1503	a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions	1494:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	3	used	used	1486:1489	arg2	material					1470:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	4	4	theme	graphene	774:781	arg1	structure					789:797	the graphene oxide structure	770:797	the graphene oxide structure	770:797	The structural characterization revealed that the Cu3(btc)2 and chitosan were incorporated into the graphene oxide structure.					
32062390	11	5	theme	btc	1455:1457	arg1	material					1470:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	5	theme	btc	1455:1457	arg1	catalyst					1496:1503	a catalyst	1494:1503	a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions	1494:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	10	6	theme	high	1357:1360	arg1	efficiencies					1374:1385	high degradation efficiencies	1357:1385	high degradation efficiencies	1357:1385	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	11	7	theme	Cu3	1451:1453	arg1	material					1470:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	7	theme	Cu3	1451:1453	arg1	catalyst					1496:1503	a catalyst	1494:1503	a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions	1494:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	8	from	degradation	1561:1571	arg1	solutions					1604:1612	aqueous solutions	1596:1612	aqueous solutions	1596:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	6	9	theme	~357.15 mg/g	991:1002	arg1	capacity					979:986	an adsorption capacity	965:986	an adsorption capacity of ~357.15 mg/g	965:1002	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	3	10	theme	polyfunctional	642:655	arg1	ligands					665:671	polyfunctional organic ligands	642:671	polyfunctional organic ligands	642:671	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	9	11	theme	Langmuir-Hinshelwood	1276:1295	arg1	plot					1303:1306	a Langmuir-Hinshelwood (L-H) plot	1274:1306	a Langmuir-Hinshelwood (L-H) plot	1274:1306	The obtained MB degradation results were fitted onto a Langmuir-Hinshelwood (L-H) plot.					
32062390	8	12	theme	GO-CS	1119:1123	arg1	material					1144:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	10	13	theme	neutral	1390:1396	arg1	conditions					1401:1410	neutral pH conditions	1390:1410	neutral pH conditions	1390:1410	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	0	14	theme	photocatalytic	95:108	arg1	degradation					110:120	photocatalytic degradation	95:120	photocatalytic degradation of methylene blue	95:138	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	9	15	theme	L-H	1298:1300	arg1	plot					1303:1306	a Langmuir-Hinshelwood (L-H) plot	1274:1306	a Langmuir-Hinshelwood (L-H) plot	1274:1306	The obtained MB degradation results were fitted onto a Langmuir-Hinshelwood (L-H) plot.					
32062390	8	16	theme	Cu3	1125:1127	arg1	material					1144:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	1	17	theme	appealing	185:193	arg1	materials					171:179	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	1	17	theme	appealing	185:193	arg1	channels					195:202	appealing channels	185:202	appealing channels for water treatment	185:222	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	8	18	theme	btc	1129:1131	arg1	material					1144:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	11	19	theme	methylene	1576:1584	arg1	degradation					1561:1571	the efficient degradation	1547:1571	the efficient degradation of methylene blue from aqueous solutions	1547:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	0	20	theme	blue	135:138	arg1	methylene					125:133	methylene blue	125:138	methylene blue	125:138	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	5	21	theme	order	907:911	arg1	model					921:925	pseudosecond order kinetic model	894:925	pseudosecond order kinetic model	894:925	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	3	22	theme	permeable	581:589	arg1	MOFs					572:575	MOFs	572:575	MOFs	572:575	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	3	22	theme	permeable	581:589	arg1	compounds					603:611	permeable crystalline compounds	581:611	permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands	581:671	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	10	23	theme	GO-CS	1313:1317	arg1	material					1338:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	8	24	theme	98	1163:1164	arg1	%					1165:1165	%	1165:1165	%	1165:1165	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	0	25	theme	hybrid	55:60	arg1	composite					62:70	hybrid composite	55:70	hybrid composite for the adsorption	55:89	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	0	25	theme	hybrid	55:60	arg1	methylene					125:133	methylene blue	125:138	methylene blue	125:138	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	1	26	theme	graphene	145:152	arg1	oxide					154:158	graphene oxide	145:158	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	2	27	theme	catalyst	442:449	arg1	material					451:458	a catalyst material	440:458	a catalyst material	440:458	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	0	28	from	Immobilization	0:13	arg1	graphene					31:38	graphene	31:38	graphene	31:38	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	4	29	theme	structural	678:687	arg1	characterization					689:704	The structural characterization	674:704	The structural characterization	674:704	The structural characterization revealed that the Cu3(btc)2 and chitosan were incorporated into the graphene oxide structure.					
32062390	2	30	theme	chitosan	398:405	arg1	oxide					421:425	chitosan (CS)/graphene oxide	398:425	chitosan (CS)/graphene oxide (GO)	398:430	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	31	theme	metal	336:340	arg1	MOF					361:363	MOF	361:363	MOF	361:363	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	31	theme	metal	336:340	arg1	framework					350:358	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework	281:358	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF)	281:364	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	5	32	theme	@	829:829	arg1	catalyst					840:847	GO-CS@Cu3(btc)2 catalyst	824:847	GO-CS@Cu3(btc)2 catalyst	824:847	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	6	33	theme	GO-CS	928:932	arg1	btc					938:940	btc	938:940	btc	938:940	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	6	33	theme	GO-CS	928:932	arg1	Cu3					934:936	GO-CS@Cu3	928:936	GO-CS@Cu3(btc)2	928:942	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	10	34	theme	@	1318:1318	arg1	material					1338:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	2	35	theme	diffusive	541:549	arg1	spectra					563:569	UV-vis diffusive reflectance spectra	534:569	UV-vis diffusive reflectance spectra	534:569	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	8	36	theme	UV	1205:1206	arg1	irradiation					1208:1218	UV irradiation	1205:1218	UV irradiation	1205:1218	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	0	37	theme	Cu3	18:20	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of Cu3(btc)2 on graphene	0:38	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	2	38	theme	/graphene	411:419	arg1	oxide					421:425	chitosan (CS)/graphene oxide	398:425	chitosan (CS)/graphene oxide (GO)	398:430	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	39	theme	CS	408:409	arg1	oxide					421:425	chitosan (CS)/graphene oxide	398:425	chitosan (CS)/graphene oxide (GO)	398:430	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	11	40	theme	catalyst	1461:1468	arg1	material					1470:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	40	theme	catalyst	1461:1468	arg1	catalyst					1496:1503	a catalyst	1494:1503	a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions	1494:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	1	41	dep	oxide	154:158	arg1	GO					161:162	GO	161:162	GO	161:162	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	2	42	dep	oxide	421:425	arg1	GO					428:429	GO	428:429	GO	428:429	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	5	43	theme	pseudosecond	894:905	arg1	model					921:925	pseudosecond order kinetic model	894:925	pseudosecond order kinetic model	894:925	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	1	44	theme	water	208:212	arg1	treatment					214:222	water treatment	208:222	water treatment	208:222	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	10	45	theme	catalyst	1329:1336	arg1	material					1338:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	3	46	theme	crystalline	591:601	arg1	MOFs					572:575	MOFs	572:575	MOFs	572:575	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	3	46	theme	crystalline	591:601	arg1	compounds					603:611	permeable crystalline compounds	581:611	permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands	581:671	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	9	47	theme	MB	1234:1235	arg1	results					1249:1255	The obtained MB degradation results	1221:1255	The obtained MB degradation results	1221:1255	The obtained MB degradation results were fitted onto a Langmuir-Hinshelwood (L-H) plot.					
32062390	7	48	theme	photocatalytic	1089:1102	arg1	processes					1104:1112	several photocatalytic processes	1081:1112	several photocatalytic processes	1081:1112	The findings displayed the probability of reusing the catalyst material for several photocatalytic processes.					
32062390	10	49	theme	degradation	1362:1372	arg1	efficiencies					1374:1385	high degradation efficiencies	1357:1385	high degradation efficiencies	1357:1385	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	3	50	theme	metal	627:631	arg1	ions					633:636	metal ions	627:636	metal ions	627:636	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	1	51	theme	oxide	154:158	arg1	materials					171:179	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	1	51	theme	oxide	154:158	arg1	channels					195:202	appealing channels	185:202	appealing channels for water treatment	185:222	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	7	52	theme	catalyst	1059:1066	arg1	material					1068:1075	the catalyst material	1055:1075	the catalyst material for several photocatalytic processes	1055:1112	The findings displayed the probability of reusing the catalyst material for several photocatalytic processes.					
32062390	1	53	from	water	247:251	arg1	separation					231:240	their separation	225:240	their separation from water for recycle	225:263	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	10	54	theme	pH	1398:1399	arg1	conditions					1401:1410	neutral pH conditions	1390:1410	neutral pH conditions	1390:1410	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	3	55	theme	organic	657:663	arg1	ligands					665:671	polyfunctional organic ligands	642:671	polyfunctional organic ligands	642:671	MOFs are permeable crystalline compounds consisting of metal ions and polyfunctional organic ligands.					
32062390	1	56	theme	-based	164:169	arg1	materials					171:179	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials	141:179	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	1	56	theme	-based	164:169	arg1	channels					195:202	appealing channels	185:202	appealing channels for water treatment	185:222	The graphene oxide (GO)-based materials are appealing channels for water treatment, their separation from water for recycle remains a task.					
32062390	6	57	theme	adsorption	968:977	arg1	capacity					979:986	an adsorption capacity	965:986	an adsorption capacity of ~357.15 mg/g	965:1002	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	11	58	theme	efficient	1551:1559	arg1	degradation					1561:1571	the efficient degradation	1547:1571	the efficient degradation of methylene blue from aqueous solutions	1547:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	0	59	theme	methylene	125:133	arg1	composite					62:70	hybrid composite	55:70	hybrid composite for the adsorption	55:89	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	0	59	theme	methylene	125:133	arg1	degradation					110:120	photocatalytic degradation	95:120	photocatalytic degradation of methylene blue	95:138	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	0	59	theme	methylene	125:133	arg1	methylene					125:133	methylene blue	125:138	methylene blue	125:138	Immobilization of Cu3(btc)2 on graphene oxide-chitosan hybrid composite for the adsorption and photocatalytic degradation of methylene blue.					
32062390	6	60	contain	possess	957:963	arg1	btc					938:940	btc	938:940	btc	938:940	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	6	60	contain	possess	957:963	arg1	Cu3					934:936	GO-CS@Cu3	928:936	GO-CS@Cu3(btc)2	928:942	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	6	60	contain	possess	957:963	arg2	capacity					979:986	an adsorption capacity	965:986	an adsorption capacity of ~357.15 mg/g	965:1002	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	8	61	theme	@	1124:1124	arg1	material					1144:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	11	62	theme	aqueous	1596:1602	arg1	solutions					1604:1612	aqueous solutions	1596:1612	aqueous solutions	1596:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	4	63	theme	oxide	783:787	arg1	structure					789:797	the graphene oxide structure	770:797	the graphene oxide structure	770:797	The structural characterization revealed that the Cu3(btc)2 and chitosan were incorporated into the graphene oxide structure.					
32062390	11	64	theme	blue	1586:1589	arg1	methylene					1576:1584	methylene blue	1576:1589	methylene blue from aqueous solutions	1576:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	5	65	theme	kinetic	913:919	arg1	model					921:925	pseudosecond order kinetic model	894:925	pseudosecond order kinetic model	894:925	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	5	66	theme	MB	818:819	arg1	adsorption					804:813	The adsorption	800:813	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst	800:847	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	8	67	theme	catalyst	1135:1142	arg1	material					1144:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material	1115:1151	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	2	68	theme	UV-vis	534:539	arg1	spectra					563:569	UV-vis diffusive reflectance spectra	534:569	UV-vis diffusive reflectance spectra	534:569	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	5	69	theme	btc	834:836	arg1	catalyst					840:847	GO-CS@Cu3(btc)2 catalyst	824:847	GO-CS@Cu3(btc)2 catalyst	824:847	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	8	70	theme	%	1165:1165	arg1	degradation					1167:1177	98% degradation	1163:1177	98% degradation of MB	1163:1183	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	11	71	from	solutions	1604:1612	arg1	degradation					1561:1571	the efficient degradation	1547:1571	the efficient degradation of methylene blue from aqueous solutions	1547:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	71	from	solutions	1604:1612	arg1	methylene					1576:1584	methylene blue	1576:1589	methylene blue from aqueous solutions	1576:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	2	72	theme	organic	342:348	arg1	MOF					361:363	MOF	361:363	MOF	361:363	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	72	theme	organic	342:348	arg1	framework					350:358	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework	281:358	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF)	281:364	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	5	73	theme	GO-CS	824:828	arg1	catalyst					840:847	GO-CS@Cu3(btc)2 catalyst	824:847	GO-CS@Cu3(btc)2 catalyst	824:847	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	6	74	theme	@	933:933	arg1	btc					938:940	btc	938:940	btc	938:940	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	6	74	theme	@	933:933	arg1	Cu3					934:936	GO-CS@Cu3	928:936	GO-CS@Cu3(btc)2	928:942	GO-CS@Cu3(btc)2 was found to possess an adsorption capacity of ~357.15 mg/g.					
32062390	2	75	theme	reflectance	551:561	arg1	spectra					563:569	UV-vis diffusive reflectance spectra	534:569	UV-vis diffusive reflectance spectra	534:569	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	5	76	theme	Cu3	830:832	arg1	catalyst					840:847	GO-CS@Cu3(btc)2 catalyst	824:847	GO-CS@Cu3(btc)2 catalyst	824:847	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	8	77	theme	MB	1182:1183	arg1	degradation					1167:1177	98% degradation	1163:1177	98% degradation of MB	1163:1183	The GO-CS@Cu3(btc)2 catalyst material exhibited 98% degradation of MB within 60 min under UV irradiation.					
32062390	5	78	theme	Langmuir	872:879	arg1	isotherm					881:888	Langmuir isotherm	872:888	Langmuir isotherm	872:888	The adsorption of MB by GO-CS@Cu3(btc)2 catalyst was clearly defined by Langmuir isotherm and pseudosecond order kinetic model.					
32062390	4	79	theme	Cu3	724:726	arg1	btc					728:730	the Cu3(btc)2	720:732	the Cu3(btc)2	720:732	The structural characterization revealed that the Cu3(btc)2 and chitosan were incorporated into the graphene oxide structure.					
32062390	10	80	theme	btc	1323:1325	arg1	material					1338:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	2	81	theme	btc	289:291	arg1	MOF					361:363	MOF	361:363	MOF	361:363	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	81	theme	btc	289:291	arg1	framework					350:358	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework	281:358	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF)	281:364	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	10	82	theme	Cu3	1319:1321	arg1	material					1338:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material	1309:1345	The GO-CS@Cu3(btc)2 catalyst material exhibited high degradation efficiencies at neutral pH conditions.					
32062390	2	83	theme	Cu3	285:287	arg1	btc					289:291	The Cu3(btc)2	281:293	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF)	281:364	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	83	theme	Cu3	285:287	arg1	acid					330:333	btc = benzene-1,3,5-tricarboxylic acid	296:333	btc = benzene-1,3,5-tricarboxylic acid	296:333	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	11	84	theme	GO-CS	1445:1449	arg1	material					1470:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	the GO-CS@Cu3(btc)2 catalyst material	1441:1477	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	11	84	theme	GO-CS	1445:1449	arg1	catalyst					1496:1503	a catalyst	1494:1503	a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions	1494:1612	The results have shown that the GO-CS@Cu3(btc)2 catalyst material can be used as a catalyst for adsorption and as a photocatalyst for the efficient degradation of methylene blue from aqueous solutions.					
32062390	2	85	theme	btc = benzene-1,3,5-tricarboxylic	296:328	arg1	btc					289:291	The Cu3(btc)2	281:293	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF)	281:364	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	2	85	theme	btc = benzene-1,3,5-tricarboxylic	296:328	arg1	acid					330:333	btc = benzene-1,3,5-tricarboxylic acid	296:333	btc = benzene-1,3,5-tricarboxylic acid	296:333	The Cu3(btc)2 (btc = benzene-1,3,5-tricarboxylic acid) metal organic framework (MOF) was covalently immobilized onto chitosan (CS)/graphene oxide (GO) to form a catalyst material, which was subjected to characterization by XRD, FTIR, SEM, TEM, BET, and UV-vis diffusive reflectance spectra.					
32062390	9	86	theme	obtained	1225:1232	arg1	results					1249:1255	The obtained MB degradation results	1221:1255	The obtained MB degradation results	1221:1255	The obtained MB degradation results were fitted onto a Langmuir-Hinshelwood (L-H) plot.					
32155970	7	0	theme	weight	1179:1184	arg1	weight					1179:1184	the initial weight	1167:1184	the initial weight	1167:1184	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	7	0	theme	weight	1179:1184	arg1	%					1154:1154	14%	1152:1154	14%	1152:1154	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	7	0	theme	weight	1179:1184	arg1	%					1162:1162	16%	1160:1162	16%	1160:1162	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	7	1	theme	simulated	1103:1111	arg1	SBF					1125:1127	SBF	1125:1127	SBF	1125:1127	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	7	1	theme	simulated	1103:1111	arg1	fluid					1118:1122	simulated body fluid	1103:1122	simulated body fluid (SBF)	1103:1128	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	2	2	theme	physical	344:351	arg1	properties					381:390	the physical, mechanical, and biological properties	340:390	the physical, mechanical, and biological properties of different materials and devices	340:425	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	9	3	from	days	1431:1434	arg1	rats					1446:1449	Wistar rats	1439:1449	Wistar rats	1439:1449	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	7	4	theme	films	1092:1096	arg1	degradation					1077:1087	The degradation	1073:1087	The degradation of films into simulated body fluid (SBF)	1073:1128	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	3	5	theme	amide-type	523:532	arg1	bond					534:537	an amide-type bond	520:537	an amide-type bond	520:537	In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond.					
32155970	9	6	theme	physical	1572:1579	arg1	blends					1581:1586	the physical blends	1568:1586	the physical blends of ox-CNO formulations	1568:1609	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	2	7	theme	carbon	283:288	arg1	nanotubes					290:298	Graphene and carbon nanotubes	270:298	Graphene and carbon nanotubes	270:298	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	4	8	from	implantation	647:658	arg1	rats					670:673	Wistar rats	663:673	Wistar rats	663:673	These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats.					
32155970	4	9	theme	Wistar	663:668	arg1	rats					670:673	Wistar rats	663:673	Wistar rats	663:673	These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats.					
32155970	9	10	theme	formulations	1598:1609	arg1	blends					1581:1586	the physical blends	1568:1586	the physical blends of ox-CNO formulations	1568:1609	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	4	11	dep	chitosan	574:581	arg1	composites					605:614	composites	605:614	composites	605:614	These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats.					
32155970	11	12	theme	future	1940:1945	arg1	impact					1947:1952	the future impact	1936:1952	the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications	1936:2019	With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.					
32155970	9	13	theme	other	1372:1376	arg1	hand					1378:1381	the other hand	1368:1381	the other hand	1368:1381	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	11	14	theme	CS/PVA/CS-g-CNO	1957:1971	arg1	films					1987:1991	CS/PVA/CS-g-CNO nanocomposite films	1957:1991	CS/PVA/CS-g-CNO nanocomposite films	1957:1991	With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.					
32155970	8	15	theme	hydrogen	1347:1354	arg1	bonding					1356:1362	hydrogen bonding	1347:1362	hydrogen bonding	1347:1362	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	6	16	theme	Scanning	949:956	arg1	DSC					971:973	DSC	971:973	DSC	971:973	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	16	theme	Scanning	949:956	arg1	Calorimetry					958:968	Differential Scanning Calorimetry	936:968	Differential Scanning Calorimetry (DSC)	936:974	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	10	17	theme	hydrolytic	1616:1625	arg1	results					1627:1633	SBF hydrolytic results	1612:1633	SBF hydrolytic results	1612:1633	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	1	18	theme	resistant	137:145	arg1	materials					147:155	biocompatible and resistant materials	119:155	biocompatible and resistant materials such as carbon nanomaterials and biopolymers	119:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	18	theme	resistant	137:145	arg1	biopolymers					190:200	biopolymers	190:200	biopolymers	190:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	18	theme	resistant	137:145	arg1	nanomaterials					172:184	carbon nanomaterials	165:184	carbon nanomaterials	165:184	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	11	19	theme	biomedical	1997:2006	arg1	applications					2008:2019	biomedical applications	1997:2019	biomedical applications	1997:2019	With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.					
32155970	2	20	theme	Graphene	270:277	arg1	nanotubes					290:298	Graphene and carbon nanotubes	270:298	Graphene and carbon nanotubes	270:298	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	8	21	theme	weight	1243:1248	arg1	loss					1250:1253	weight loss	1243:1253	weight loss	1243:1253	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	7	22	theme	initial	1171:1177	arg1	weight					1179:1184	the initial weight	1167:1184	the initial weight	1167:1184	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	10	23	theme	ox-CNO	1721:1726	arg1	content					1728:1734	with a higher ox-CNO content	1707:1734	with a higher ox-CNO content	1707:1734	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	1	24	theme	carbon	165:170	arg1	nanomaterials					172:184	carbon nanomaterials	165:184	carbon nanomaterials	165:184	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	6	25	theme	Diffraction	1001:1011	arg1	Spectroscopy					1013:1024	X-ray Diffraction Spectroscopy	995:1024	X-ray Diffraction Spectroscopy (XRD)	995:1030	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	25	theme	Diffraction	1001:1011	arg1	XRD					1027:1029	XRD	1027:1029	XRD	1027:1029	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	1	26	theme	biocompatible	119:131	arg1	materials					147:155	biocompatible and resistant materials	119:155	biocompatible and resistant materials such as carbon nanomaterials and biopolymers	119:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	26	theme	biocompatible	119:131	arg1	biopolymers					190:200	biopolymers	190:200	biopolymers	190:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	26	theme	biocompatible	119:131	arg1	nanomaterials					172:184	carbon nanomaterials	165:184	carbon nanomaterials	165:184	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	5	27	theme	polyvinyl	722:730	arg1	alcohol					732:738	polyvinyl alcohol	722:738	polyvinyl alcohol	722:738	Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes.					
32155970	9	28	theme	Wistar	1439:1444	arg1	rats					1446:1449	Wistar rats	1439:1449	Wistar rats	1439:1449	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	6	29	theme	Tensile	977:983	arg1	strength					985:992	Tensile strength	977:992	Tensile strength	977:992	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	9	30	dep	in	1384:1385	arg1	vivo					1387:1390	vivo	1387:1390	vivo	1387:1390	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	1	31	from	materials	147:155	arg1	scaffolding					102:112	scaffolding	102:112	scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers	102:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	31	from	materials	147:155	arg1	design					92:97	The design	88:97	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers	88:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	2	32	theme	biological	370:379	arg1	properties					381:390	the physical, mechanical, and biological properties	340:390	the physical, mechanical, and biological properties of different materials and devices	340:425	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	0	33	theme	Nanocomposite	0:12	arg1	Films					14:18	Nanocomposite Films	0:18	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.	0:86	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.					
32155970	1	34	theme	scaffolding	102:112	arg1	design					92:97	The design	88:97	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers	88:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	6	35	theme	Electron	1046:1053	arg1	Microscopy					1055:1064	Scanning Electron Microscopy	1037:1064	Scanning Electron Microscopy (SEM)	1037:1070	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	35	theme	Electron	1046:1053	arg1	SEM					1067:1069	SEM	1067:1069	SEM	1067:1069	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	2	36	theme	devices	419:425	arg1	properties					381:390	the physical, mechanical, and biological properties	340:390	the physical, mechanical, and biological properties of different materials and devices	340:425	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	9	37	theme	normal	1482:1487	arg1	resorption					1489:1498	normal resorption	1482:1498	normal resorption of the materials	1482:1515	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	8	38	theme	due	1312:1314	arg1	CS-g-CNO					1303:1310	CS-g-CNO	1303:1310	CS-g-CNO due to a higher SBF interaction by hydrogen bonding	1303:1362	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	2	39	theme	materials	405:413	arg1	properties					381:390	the physical, mechanical, and biological properties	340:390	the physical, mechanical, and biological properties of different materials and devices	340:425	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	0	40	theme	Carbon	40:45	arg1	Nano-Onions					47:57	Chitosan-Grafted Carbon Nano-Onions	23:57	Chitosan-Grafted Carbon Nano-Onions	23:57	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.					
32155970	6	41	theme	Infrared	872:879	arg1	FTIR					895:898	FTIR	895:898	FTIR	895:898	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	41	theme	Infrared	872:879	arg1	Spectroscopy					881:892	Fourier Transformation Infrared Spectroscopy	849:892	Fourier Transformation Infrared Spectroscopy (FTIR)	849:899	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	5	42	theme	carbon	745:750	arg1	nano-onions					752:762	carbon nano-onions	745:762	carbon nano-onions	745:762	Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes.					
32155970	3	43	theme	carbon	468:473	arg1	nano-onions					475:485	carbon nano-onions	468:485	carbon nano-onions	468:485	In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond.					
32155970	5	44	theme	comparison	787:796	arg1	purposes					798:805	comparison purposes	787:805	comparison purposes	787:805	Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes.					
32155970	6	45	theme	Fourier	849:855	arg1	FTIR					895:898	FTIR	895:898	FTIR	895:898	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	45	theme	Fourier	849:855	arg1	Spectroscopy					881:892	Fourier Transformation Infrared Spectroscopy	849:892	Fourier Transformation Infrared Spectroscopy (FTIR)	849:899	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	9	46	theme	materials	1507:1515	arg1	resorption					1489:1498	normal resorption	1482:1498	normal resorption of the materials	1482:1515	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	8	47	theme	SBF	1328:1330	arg1	interaction					1332:1342	a higher SBF interaction	1319:1342	a higher SBF interaction by hydrogen bonding	1319:1362	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	1	48	theme	high	239:242	arg1	rate					244:247	the high rate	235:247	the high rate of injured patients	235:267	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	10	49	theme	in	1651:1652	arg1	degradation					1659:1669	the in vivo degradation	1647:1669	the in vivo degradation for all samples	1647:1685	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	9	50	theme	lower	1522:1526	arg1	processes					1541:1549	lower inflammation processes	1522:1549	lower inflammation processes	1522:1549	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	7	51	theme	treatment	1203:1211	arg1	days					1195:1198	25 days	1192:1198	25 days of treatment	1192:1211	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	0	52	theme	Biomedical	63:72	arg1	Applications					74:85	Biomedical Applications	63:85	Biomedical Applications	63:85	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.					
32155970	1	53	from	design	92:97	arg1	materials					147:155	biocompatible and resistant materials	119:155	biocompatible and resistant materials such as carbon nanomaterials and biopolymers	119:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	53	from	design	92:97	arg1	biopolymers					190:200	biopolymers	190:200	biopolymers	190:200	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	53	from	design	92:97	arg1	nanomaterials					172:184	carbon nanomaterials	165:184	carbon nanomaterials	165:184	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	1	54	theme	patients	260:267	arg1	rate					244:247	the high rate	235:247	the high rate of injured patients	235:267	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
32155970	6	55	theme	Film	808:811	arg1	characterization					813:828	Film characterization	808:828	Film characterization	808:828	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	56	theme	Thermogravimetric	902:918	arg1	TGA					930:932	TGA	930:932	TGA	930:932	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	56	theme	Thermogravimetric	902:918	arg1	Analysis					920:927	Thermogravimetric Analysis	902:927	Thermogravimetric Analysis (TGA)	902:933	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	10	57	theme	greater	1837:1843	arg1	reabsorption					1845:1856	greater reabsorption	1837:1856	greater reabsorption	1837:1856	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	3	58	with	grafting	456:463	arg1	chitosan					492:499	chitosan	492:499	chitosan (CS-g-CNO) through an amide-type bond	492:537	In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond.					
32155970	3	58	with	grafting	456:463	arg1	CS-g-CNO					502:509	CS-g-CNO	502:509	CS-g-CNO	502:509	In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond.					
32155970	4	59	theme	subdermal	637:645	arg1	implantation					647:658	subdermal implantation	637:658	subdermal implantation in Wistar rats	637:673	These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats.					
32155970	9	60	theme	ox-CNO	1591:1596	arg1	formulations					1598:1609	ox-CNO formulations	1591:1609	ox-CNO formulations	1591:1609	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	5	61	theme	physical	687:694	arg1	mixture					696:702	physical mixture	687:702	physical mixture	687:702	Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes.					
32155970	10	62	theme	SBF	1612:1614	arg1	results					1627:1633	SBF hydrolytic results	1612:1633	SBF hydrolytic results	1612:1633	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	10	63	theme	higher	1714:1719	arg1	content					1728:1734	with a higher ox-CNO content	1707:1734	with a higher ox-CNO content	1707:1734	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	11	64	theme	nanocomposite	1973:1985	arg1	films					1987:1991	CS/PVA/CS-g-CNO nanocomposite films	1957:1991	CS/PVA/CS-g-CNO nanocomposite films	1957:1991	With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.					
32155970	10	65	theme	with	1707:1710	arg1	content					1728:1734	with a higher ox-CNO content	1707:1734	with a higher ox-CNO content	1707:1734	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	9	66	theme	in	1384:1385	arg1	evaluation					1392:1401	in vivo evaluation	1384:1401	in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue	1384:1467	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	6	67	theme	Differential	936:947	arg1	DSC					971:973	DSC	971:973	DSC	971:973	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	67	theme	Differential	936:947	arg1	Calorimetry					958:968	Differential Scanning Calorimetry	936:968	Differential Scanning Calorimetry (DSC)	936:974	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	11	68	theme	films	1987:1991	arg1	impact					1947:1952	the future impact	1936:1952	the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications	1936:2019	With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.					
32155970	8	69	theme	faster	1223:1228	arg1	degradation					1230:1240	a faster degradation	1221:1240	a faster degradation (weight loss and pH changes)	1221:1269	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	9	70	theme	nanocomposites	1406:1419	arg1	evaluation					1392:1401	in vivo evaluation	1384:1401	in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue	1384:1467	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	10	71	dep	in	1651:1652	arg1	vivo					1654:1657	vivo	1654:1657	vivo	1654:1657	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	6	72	theme	X-ray	995:999	arg1	Spectroscopy					1013:1024	X-ray Diffraction Spectroscopy	995:1024	X-ray Diffraction Spectroscopy (XRD)	995:1030	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	72	theme	X-ray	995:999	arg1	XRD					1027:1029	XRD	1027:1029	XRD	1027:1029	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	8	73	theme	pH	1259:1260	arg1	changes					1262:1268	pH changes	1259:1268	pH changes	1259:1268	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	5	74	with	Films	676:680	arg1	mixture					696:702	physical mixture	687:702	physical mixture	687:702	Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes.					
32155970	2	75	used	used	324:327	arg2	nanotubes					290:298	Graphene and carbon nanotubes	270:298	Graphene and carbon nanotubes	270:298	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	9	76	theme	subdermal	1452:1460	arg1	tissue					1462:1467	subdermal tissue	1452:1467	subdermal tissue	1452:1467	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	9	76	theme	subdermal	1452:1460	arg1	days					1431:1434	30 days	1428:1434	30 days in Wistar rats	1428:1449	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	0	77	theme	Chitosan-Grafted	23:38	arg1	Nano-Onions					47:57	Chitosan-Grafted Carbon Nano-Onions	23:57	Chitosan-Grafted Carbon Nano-Onions	23:57	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.					
32155970	6	78	theme	Scanning	1037:1044	arg1	Microscopy					1055:1064	Scanning Electron Microscopy	1037:1064	Scanning Electron Microscopy (SEM)	1037:1070	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	78	theme	Scanning	1037:1044	arg1	SEM					1067:1069	SEM	1067:1069	SEM	1067:1069	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	10	79	theme	material	1767:1774	arg1	stability					1750:1758	the stability	1746:1758	the stability of the material	1746:1774	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	0	80	theme	Nano-Onions	47:57	arg1	Films					14:18	Nanocomposite Films	0:18	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.	0:86	Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications.					
32155970	8	81	theme	CS-g-CNO	1303:1310	arg1	CS-g-CNO					1303:1310	CS-g-CNO	1303:1310	CS-g-CNO due to a higher SBF interaction by hydrogen bonding	1303:1362	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	8	81	theme	CS-g-CNO	1303:1310	arg1	composites					1289:1298	composites	1289:1298	composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding	1289:1362	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	2	82	theme	different	395:403	arg1	materials					405:413	different materials	395:413	different materials	395:413	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	7	83	theme	body	1113:1116	arg1	SBF					1125:1127	SBF	1125:1127	SBF	1125:1127	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	7	83	theme	body	1113:1116	arg1	fluid					1118:1122	simulated body fluid	1103:1122	simulated body fluid (SBF)	1103:1128	The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment.					
32155970	6	84	theme	Transformation	857:870	arg1	FTIR					895:898	FTIR	895:898	FTIR	895:898	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	6	84	theme	Transformation	857:870	arg1	Spectroscopy					881:892	Fourier Transformation Infrared Spectroscopy	849:892	Fourier Transformation Infrared Spectroscopy (FTIR)	849:899	Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM).					
32155970	10	85	theme	degradation	1794:1804	arg1	capacity					1806:1813	its degradation capacity	1790:1813	its degradation capacity	1790:1813	SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO.					
32155970	3	86	theme	nano-onions	475:485	arg1	grafting					456:463	the grafting	452:463	the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond	452:537	In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond.					
32155970	8	87	dep	degradation	1230:1240	arg1	loss					1250:1253	weight loss	1243:1253	weight loss	1243:1253	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	8	87	dep	degradation	1230:1240	arg1	changes					1262:1268	pH changes	1259:1268	pH changes	1259:1268	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	4	88	theme	polyvinyl	587:595	arg1	alcohol					597:603	polyvinyl alcohol	587:603	polyvinyl alcohol	587:603	These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats.					
32155970	8	89	theme	higher	1321:1326	arg1	interaction					1332:1342	a higher SBF interaction	1319:1342	a higher SBF interaction by hydrogen bonding	1319:1362	Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding.					
32155970	9	90	theme	inflammation	1528:1539	arg1	processes					1541:1549	lower inflammation processes	1522:1549	lower inflammation processes	1522:1549	On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations.					
32155970	2	91	theme	mechanical	354:363	arg1	properties					381:390	the physical, mechanical, and biological properties	340:390	the physical, mechanical, and biological properties of different materials and devices	340:425	Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices.					
32155970	1	92	theme	injured	252:258	arg1	patients					260:267	injured patients	252:267	injured patients	252:267	The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients.					
33049863	0	0	dep	acidophilus	108:118	arg1	016					120:122	016	120:122	Lactobacillus acidophilus 016	94:122	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	7	1	theme	viability	1084:1092	arg1	studies					1094:1100	the viability studies	1080:1100	the viability studies	1080:1100	Further, the viability studies confirmed the survival of 71% population during the storage at 35 °C.					
33049863	5	2	theme	L.	843:844	arg1	016					858:860	L. acidophilus 016	843:860	L. acidophilus 016	843:860	L. acidophilus 016 was successfully immobilized onto the BCNF through the adsorption-incubation technique.					
33049863	4	3	theme	tensile	659:665	arg1	strength					667:674	tensile strength	659:674	tensile strength	659:674	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	2	4	theme	solution	469:476	arg1	volume					435:440	an equal volume	426:440	an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning	426:518	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	5	5	theme	adsorption-incubation	917:937	arg1	technique					939:947	the adsorption-incubation technique	913:947	the adsorption-incubation technique	913:947	L. acidophilus 016 was successfully immobilized onto the BCNF through the adsorption-incubation technique.					
33049863	2	6	theme	Bacterial	335:343	arg1	cellulose					345:353	Bacterial cellulose	335:353	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution	335:407	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	2	6	theme	Bacterial	335:343	arg1	%					357:357	5%	356:357	5%	356:357	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	6	7	theme	SEM	950:952	arg1	micrograph					954:963	SEM micrograph	950:963	SEM micrograph	950:963	SEM micrograph revealed that the immobilized bacteria sustained without any damage during the storage for up to 24 days.					
33049863	8	8	theme	various	1248:1254	arg1	applications					1267:1278	various commercial applications	1248:1278	various commercial applications	1248:1278	These observations recommended the possibility of BCNF based probiotics for various commercial applications.					
33049863	7	9	theme	71	1128:1129	arg1	%					1130:1130	%	1130:1130	%	1130:1130	Further, the viability studies confirmed the survival of 71% population during the storage at 35 °C.					
33049863	4	10	theme	thermal	727:733	arg1	analysis					735:742	thermal analysis	727:742	thermal analysis of BCNF	727:750	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	7	11	theme	population	1132:1141	arg1	survival					1116:1123	the survival	1112:1123	the survival of 71% population	1112:1141	Further, the viability studies confirmed the survival of 71% population during the storage at 35 °C.					
33049863	0	12	theme	Cellulose	10:18	arg1	BCNF					32:35	BCNF	32:35	BCNF	32:35	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	0	12	theme	Cellulose	10:18	arg1	Fiber					25:29	Bacterial Cellulose Nano Fiber	0:29	Bacterial Cellulose Nano Fiber (BCNF) as carrier	0:47	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	1	13	theme	carrier	239:245	arg1	ability					226:232	its ability	222:232	its ability	222:232	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	1	13	theme	carrier	239:245	arg1	material					247:254	a carrier material	237:254	a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016	237:332	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	3	14	theme	Fourier-transform	521:537	arg1	spectra					548:554	Fourier-transform infrared spectra	521:554	Fourier-transform infrared spectra of BCNF	521:562	Fourier-transform infrared spectra of BCNF confirmed the absence of TFA used in the dissolution process.					
33049863	7	15	theme	%	1130:1130	arg1	population					1132:1141	71% population	1128:1141	71% population	1128:1141	Further, the viability studies confirmed the survival of 71% population during the storage at 35 °C.					
33049863	0	16	theme	Bacterial	0:8	arg1	BCNF					32:35	BCNF	32:35	BCNF	32:35	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	0	16	theme	Bacterial	0:8	arg1	Fiber					25:29	Bacterial Cellulose Nano Fiber	0:29	Bacterial Cellulose Nano Fiber (BCNF) as carrier	0:47	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	1	17	dep	acidophilus	318:328	arg1	016					330:332	016	330:332	the probiotic bacteria Lactobacillus acidophilus 016	281:332	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	3	18	theme	infrared	539:546	arg1	spectra					548:554	Fourier-transform infrared spectra	521:554	Fourier-transform infrared spectra of BCNF	521:562	Fourier-transform infrared spectra of BCNF confirmed the absence of TFA used in the dissolution process.					
33049863	1	19	theme	bacterial	168:176	arg1	nanofibers					188:197	bacterial cellulose nanofibers	168:197	bacterial cellulose nanofibers (BCNF)	168:204	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	1	19	theme	bacterial	168:176	arg1	BCNF					200:203	BCNF	200:203	BCNF	200:203	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	0	20	theme	acidophilus	108:118	arg1	immobilization					65:78	the immobilization	61:78	the immobilization of probiotic, Lactobacillus acidophilus 016	61:122	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	4	21	theme	pore	708:711	arg1	diameter					713:720	pore diameter	708:720	pore diameter	708:720	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	6	22	dep	24	1062:1063	arg1	to					1059:1060	to	1059:1060	to	1059:1060	SEM micrograph revealed that the immobilized bacteria sustained without any damage during the storage for up to 24 days.					
33049863	1	23	theme	cellulose	178:186	arg1	nanofibers					188:197	bacterial cellulose nanofibers	168:197	bacterial cellulose nanofibers (BCNF)	168:204	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	1	23	theme	cellulose	178:186	arg1	BCNF					200:203	BCNF	200:203	BCNF	200:203	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	0	24	theme	Nano	20:23	arg1	BCNF					32:35	BCNF	32:35	BCNF	32:35	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	0	24	theme	Nano	20:23	arg1	Fiber					25:29	Bacterial Cellulose Nano Fiber	0:29	Bacterial Cellulose Nano Fiber (BCNF) as carrier	0:47	Bacterial Cellulose Nano Fiber (BCNF) as carrier support for the immobilization of probiotic, Lactobacillus acidophilus 016.					
33049863	1	25	dep	bacteria	295:302	arg1	acidophilus					318:328	Lactobacillus acidophilus	304:328	the probiotic bacteria Lactobacillus acidophilus 016	281:332	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	5	26	theme	acidophilus	846:856	arg1	016					858:860	L. acidophilus 016	843:860	L. acidophilus 016	843:860	L. acidophilus 016 was successfully immobilized onto the BCNF through the adsorption-incubation technique.					
33049863	2	27	theme	acid	389:392	arg1	solution					400:407	trifluoroacetic acid (TFA) solution	373:407	trifluoroacetic acid (TFA) solution	373:407	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	2	28	theme	equal	429:433	arg1	volume					435:440	an equal volume	426:440	an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning	426:518	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	3	29	theme	dissolution	605:615	arg1	process					617:623	the dissolution process	601:623	the dissolution process	601:623	Fourier-transform infrared spectra of BCNF confirmed the absence of TFA used in the dissolution process.					
33049863	3	30	theme	BCNF	559:562	arg1	spectra					548:554	Fourier-transform infrared spectra	521:554	Fourier-transform infrared spectra of BCNF	521:562	Fourier-transform infrared spectra of BCNF confirmed the absence of TFA used in the dissolution process.					
33049863	4	31	theme	Mechanical	626:635	arg1	pore-volume					691:701	pore-volume	691:701	pore-volume	691:701	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	31	theme	Mechanical	626:635	arg1	strength					667:674	tensile strength	659:674	tensile strength	659:674	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	31	theme	Mechanical	626:635	arg1	area					685:688	surface area	677:688	surface area	677:688	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	31	theme	Mechanical	626:635	arg1	diameter					713:720	pore diameter	708:720	pore diameter	708:720	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	31	theme	Mechanical	626:635	arg1	properties					637:646	Mechanical properties	626:646	Mechanical properties	626:646	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	2	32	theme	trifluoroacetic	373:387	arg1	TFA					395:397	TFA	395:397	TFA	395:397	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	2	32	theme	trifluoroacetic	373:387	arg1	acid					389:392	trifluoroacetic acid	373:392	trifluoroacetic acid (TFA) solution	373:407	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	8	33	theme	probiotics	1233:1242	arg1	possibility					1207:1217	the possibility	1203:1217	the possibility of BCNF based probiotics for various commercial applications	1203:1278	These observations recommended the possibility of BCNF based probiotics for various commercial applications.					
33049863	8	34	theme	based	1227:1231	arg1	probiotics					1233:1242	BCNF based probiotics	1222:1242	BCNF based probiotics	1222:1242	These observations recommended the possibility of BCNF based probiotics for various commercial applications.					
33049863	4	35	theme	surface	677:683	arg1	area					685:688	surface area	677:688	surface area	677:688	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	36	theme	probiotics	831:840	arg1	delivery					819:826	the delivery	815:826	the delivery of probiotics	815:840	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	1	37	theme	probiotic	285:293	arg1	bacteria					295:302	the probiotic bacteria Lactobacillus acidophilus 016	281:332	the probiotic bacteria Lactobacillus acidophilus 016	281:332	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	7	38	from	°C	1168:1169	arg1	storage					1154:1160	the storage	1150:1160	the storage at 35 °C	1150:1169	Further, the viability studies confirmed the survival of 71% population during the storage at 35 °C.					
33049863	3	39	theme	TFA	589:591	arg1	absence					578:584	the absence	574:584	the absence of TFA used in the dissolution process	574:623	Fourier-transform infrared spectra of BCNF confirmed the absence of TFA used in the dissolution process.					
33049863	8	40	theme	commercial	1256:1265	arg1	applications					1267:1278	various commercial applications	1248:1278	various commercial applications	1248:1278	These observations recommended the possibility of BCNF based probiotics for various commercial applications.					
33049863	2	41	theme	alcohol	455:461	arg1	solution					469:476	polyvinyl alcohol (PVA) solution	445:476	polyvinyl alcohol (PVA) solution	445:476	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	8	42	theme	BCNF	1222:1225	arg1	probiotics					1233:1242	BCNF based probiotics	1222:1242	BCNF based probiotics	1222:1242	These observations recommended the possibility of BCNF based probiotics for various commercial applications.					
33049863	6	43	theme	immobilized	983:993	arg1	bacteria					995:1002	the immobilized bacteria	979:1002	the immobilized bacteria	979:1002	SEM micrograph revealed that the immobilized bacteria sustained without any damage during the storage for up to 24 days.					
33049863	2	44	theme	polyvinyl	445:453	arg1	PVA					464:466	PVA	464:466	PVA	464:466	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	2	44	theme	polyvinyl	445:453	arg1	alcohol					455:461	polyvinyl alcohol	445:461	polyvinyl alcohol (PVA) solution	445:476	Bacterial cellulose (5%) dissolved in trifluoroacetic acid (TFA) solution was amended with an equal volume of polyvinyl alcohol (PVA) solution to produce nanofibers via electrospinning.					
33049863	1	45	theme	bacteria	295:302	arg1	incorporation					264:276	the incorporation	260:276	the incorporation of the probiotic bacteria Lactobacillus acidophilus 016	260:332	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
33049863	4	46	theme	BCNF	747:750	arg1	area					685:688	surface area	677:688	surface area	677:688	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	46	theme	BCNF	747:750	arg1	diameter					713:720	pore diameter	708:720	pore diameter	708:720	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	46	theme	BCNF	747:750	arg1	pore-volume					691:701	pore-volume	691:701	pore-volume	691:701	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	46	theme	BCNF	747:750	arg1	strength					667:674	tensile strength	659:674	tensile strength	659:674	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	46	theme	BCNF	747:750	arg1	analysis					735:742	thermal analysis	727:742	thermal analysis of BCNF	727:750	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	4	46	theme	BCNF	747:750	arg1	properties					637:646	Mechanical properties	626:646	Mechanical properties	626:646	Mechanical properties, including tensile strength, surface area, pore-volume, and pore diameter, and thermal analysis of BCNF revealed that the nanofibers could be incorporated in food for the delivery of probiotics.					
33049863	1	47	theme	present	129:135	arg1	study					137:141	The present study	125:141	The present study	125:141	The present study was conducted to develop bacterial cellulose nanofibers (BCNF) and to evaluate its ability as a carrier material for the incorporation of the probiotic bacteria Lactobacillus acidophilus 016.					
32858980	1	0	theme	spectroscopic	307:319	arg1	approaches					337:346	spectroscopic and microscopic approaches	307:346	spectroscopic and microscopic approaches	307:346	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	7	1	theme	1,3-β-d-glucan	1501:1514	arg1	sample					1524:1529	the 1,3-β-d-glucan polymer sample	1497:1529	the 1,3-β-d-glucan polymer sample gelled at 80 °C	1497:1545	This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.					
32858980	6	2	dep	entrapment	1400:1409	arg1	e.g.					1394:1397	e.g.	1394:1397	e.g.	1394:1397	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	3	theme	Described	1068:1076	arg1	techniques					1078:1087	Described techniques	1068:1087	Described techniques	1068:1087	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	0	4	theme	Curdlan	96:102	arg1	Matrix					104:109	the Curdlan Matrix	92:109	the Curdlan Matrix	92:109	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	1	5	theme	microscopic	325:335	arg1	approaches					337:346	spectroscopic and microscopic approaches	307:346	spectroscopic and microscopic approaches	307:346	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	4	6	theme	matrices	910:917	arg1	wettability					867:877	surface wettability	859:877	surface wettability	859:877	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	4	6	theme	matrices	910:917	arg1	energy					896:901	surface free energy	883:901	surface free energy	883:901	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	5	7	theme	electron	1041:1048	arg1	SEM					1062:1064	SEM	1062:1064	SEM	1062:1064	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	5	7	theme	electron	1041:1048	arg1	microscopy					1050:1059	scanning electron microscopy	1032:1059	scanning electron microscopy (SEM)	1032:1065	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	3	8	theme	surface	692:698	arg1	study					700:704	the surface study	688:704	the surface study	688:704	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	6	9	theme	1,3-β-d-glucan	1238:1251	arg1	case					1218:1221	the case	1214:1221	the case of the studied 1,3-β-d-glucan	1214:1251	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	2	10	theme	functional	530:539	arg1	groups					541:546	functional groups	530:546	functional groups	530:546	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	6	11	theme	polysaccharide	1269:1282	arg1	matrices					1284:1291	the organic polysaccharide matrices	1257:1291	the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1257:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	7	12	theme	different	1564:1572	arg1	structure					1574:1582	a distinctly different structure	1551:1582	a distinctly different structure	1551:1582	This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.					
32858980	3	13	theme	elemental	744:752	arg1	composition					767:777	the elemental and chemical composition	740:777	composition	767:777	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	6	14	theme	organic	1261:1267	arg1	matrices					1284:1291	the organic polysaccharide matrices	1257:1291	the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1257:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	5	15	theme	atomic	998:1003	arg1	AFM					1023:1025	AFM	1023:1025	AFM	1023:1025	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	5	15	theme	atomic	998:1003	arg1	microscopy					1011:1020	atomic force microscopy	998:1020	atomic force microscopy (AFM)	998:1026	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	6	16	dep	processes	1164:1172	arg1	such					1175:1178	such	1175:1178	such	1175:1178	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	0	17	theme	Matrix	104:109	arg1	Properties					78:87	Physicochemical Properties	62:87	Physicochemical Properties	62:87	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	0	17	theme	Matrix	104:109	arg1	Structure					48:56	Molecular Structure	38:56	Molecular Structure	38:56	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	5	18	theme	force	1005:1009	arg1	AFM					1023:1025	AFM	1023:1025	AFM	1023:1025	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	5	18	theme	force	1005:1009	arg1	microscopy					1011:1020	atomic force microscopy	998:1020	atomic force microscopy (AFM)	998:1026	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	2	19	theme	reflection	378:387	arg1	techniques					485:494	well-established techniques	468:494	well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes	468:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	2	19	theme	reflection	378:387	arg1	Fourier					389:395	Attenuated total reflection Fourier	361:395	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy	361:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	6	20	theme	tissue	1454:1459	arg1	regeneration					1461:1472	tissue regeneration	1454:1472	tissue regeneration	1454:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	3	21	theme	chemical	758:765	arg1	composition					767:777	the elemental and chemical composition	740:777	composition	767:777	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	4	22	theme	free	891:894	arg1	energy					896:901	surface free energy	883:901	surface free energy	883:901	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	6	23	theme	studied	1230:1236	arg1	1,3-β-d-glucan					1238:1251	the studied 1,3-β-d-glucan	1226:1251	the studied 1,3-β-d-glucan	1226:1251	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	24	from	temperature	1187:1197	arg1	case					1218:1221	the case	1214:1221	the case of the studied 1,3-β-d-glucan	1214:1251	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	2	25	theme	total	372:376	arg1	techniques					485:494	well-established techniques	468:494	well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes	468:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	2	25	theme	total	372:376	arg1	Fourier					389:395	Attenuated total reflection Fourier	361:395	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy	361:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	1	26	theme	structural	245:254	arg1	arrangements					256:267	the structural arrangements	241:267	the structural arrangements in 1,3-β-d-glucan (curdlan) matrices	241:304	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	27	from	arrangements	256:267	arg1	matrices					297:304	1,3-β-d-glucan (curdlan) matrices	272:304	1,3-β-d-glucan (curdlan) matrices	272:304	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	3	28	with	composition	767:777	arg1	sensitivity					797:807	high surface sensitivity	784:807	high surface sensitivity	784:807	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	2	29	theme	Attenuated	361:370	arg1	techniques					485:494	well-established techniques	468:494	well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes	468:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	2	29	theme	Attenuated	361:370	arg1	Fourier					389:395	Attenuated total reflection Fourier	361:395	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy	361:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	0	30	theme	Temperature	19:29	arg1	Effect					0:5	Effect	0:5	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix	0:109	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	6	31	theme	wide	1355:1358	arg1	range					1360:1364	wide range	1355:1364	wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1355:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	5	32	theme	microscopy	1050:1059	arg1	use					991:993	the use	987:993	the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM)	987:1065	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	6	33	theme	processes	1164:1172	arg1	modification					1122:1133	modification	1122:1133	modification	1122:1133	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	33	theme	processes	1164:1172	arg1	design					1140:1145	design	1140:1145	design	1140:1145	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	33	theme	processes	1164:1172	arg1	optimization					1108:1119	the optimization	1104:1119	the optimization	1104:1119	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	3	34	theme	photoelectron	607:619	arg1	XPS					635:637	XPS	635:637	XPS	635:637	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	3	34	theme	photoelectron	607:619	arg1	spectroscopy					621:632	X-ray photoelectron spectroscopy	601:632	X-ray photoelectron spectroscopy (XPS)	601:638	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	3	34	theme	photoelectron	607:619	arg1	method					669:674	a quantitative analytical method	643:674	a quantitative analytical method	643:674	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	1	35	theme	different	188:196	arg1	temperatures					207:218	different gelation temperatures	188:218	different gelation temperatures (80 °C and 90 °C)	188:236	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	35	theme	different	188:196	arg1	°C					224:225	80 °C and 90 °C	221:235	°C	224:225	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	35	theme	different	188:196	arg1	°C					234:235	80 °C and 90 °C	221:235	°C	234:235	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	0	36	theme	Gelation	10:17	arg1	Temperature					19:29	Gelation Temperature	10:29	Gelation Temperature	10:29	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	0	37	theme	Spectroscopic	112:124	arg1	Analyses					142:149	Spectroscopic and Microscopic Analyses	112:149	Spectroscopic and Microscopic Analyses	112:149	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	3	38	theme	analytical	658:667	arg1	spectroscopy					621:632	X-ray photoelectron spectroscopy	601:632	X-ray photoelectron spectroscopy (XPS)	601:638	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	3	38	theme	analytical	658:667	arg1	method					669:674	a quantitative analytical method	643:674	a quantitative analytical method	643:674	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	6	39	theme	manufacturing	1150:1162	arg1	processes					1164:1172	manufacturing processes	1150:1172	manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1150:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	1	40	theme	gelation	198:205	arg1	temperatures					207:218	different gelation temperatures	188:218	different gelation temperatures (80 °C and 90 °C)	188:236	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	40	theme	gelation	198:205	arg1	°C					224:225	80 °C and 90 °C	221:235	°C	224:225	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	40	theme	gelation	198:205	arg1	°C					234:235	80 °C and 90 °C	221:235	°C	234:235	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	2	41	theme	well-established	468:483	arg1	techniques					485:494	well-established techniques	468:494	well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes	468:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	2	41	theme	well-established	468:483	arg1	Fourier					389:395	Attenuated total reflection Fourier	361:395	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy	361:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	1	42	theme	1,3-β-d-glucan	272:285	arg1	matrices					297:304	1,3-β-d-glucan (curdlan) matrices	272:304	1,3-β-d-glucan (curdlan) matrices	272:304	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	2	43	theme	organic	551:557	arg1	molecules					559:567	organic molecules	551:567	organic molecules based on their vibration modes	551:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	0	44	dep	Structure	48:56	arg1	the					34:36	the	34:36	the	34:36	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	6	45	theme	gelation	1202:1209	arg1	temperature					1187:1197	the temperature	1183:1197	the temperature of gelation in the case of the studied 1,3-β-d-glucan	1183:1251	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	1	46	theme	temperatures	207:218	arg1	effect					178:183	the effect	174:183	the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices	174:304	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	0	47	theme	Microscopic	130:140	arg1	Analyses					142:149	Spectroscopic and Microscopic Analyses	112:149	Spectroscopic and Microscopic Analyses	112:149	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	2	48	dep	transform	397:405	arg1	infrared					407:414	infrared	407:414	transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy	397:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	7	49	contain	has	1547:1549	arg1	sample					1524:1529	the 1,3-β-d-glucan polymer sample	1497:1529	the 1,3-β-d-glucan polymer sample gelled at 80 °C	1497:1545	This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.					
32858980	7	49	contain	has	1547:1549	arg2	structure					1574:1582	a distinctly different structure	1551:1582	a distinctly different structure	1551:1582	This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.					
32858980	6	50	theme	drugs	1414:1418	arg1	production					1423:1432	production	1423:1432	production of biomaterials for tissue regeneration	1423:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	50	theme	drugs	1414:1418	arg1	entrapment					1400:1409	entrapment	1400:1409	entrapment of drugs	1400:1418	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	1	51	theme	curdlan	288:294	arg1	matrices					297:304	1,3-β-d-glucan (curdlan) matrices	272:304	1,3-β-d-glucan (curdlan) matrices	272:304	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	6	52	theme	applications	1380:1391	arg1	characteristics					1335:1349	desired characteristics	1327:1349	desired characteristics	1327:1349	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	52	theme	applications	1380:1391	arg1	range					1360:1364	wide range	1355:1364	wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1355:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	3	53	theme	quantitative	645:656	arg1	spectroscopy					621:632	X-ray photoelectron spectroscopy	601:632	X-ray photoelectron spectroscopy (XPS)	601:638	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	3	53	theme	quantitative	645:656	arg1	method					669:674	a quantitative analytical method	643:674	a quantitative analytical method	643:674	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	4	54	theme	surface	859:865	arg1	wettability					867:877	surface wettability	859:877	surface wettability	859:877	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	4	55	theme	surface	883:889	arg1	energy					896:901	surface free energy	883:901	surface free energy	883:901	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	0	56	theme	Molecular	38:46	arg1	Structure					48:56	Molecular Structure	38:56	Molecular Structure	38:56	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	3	57	theme	high	784:787	arg1	sensitivity					797:807	high surface sensitivity	784:807	high surface sensitivity	784:807	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	2	58	theme	vibration	584:592	arg1	modes					594:598	their vibration modes	578:598	their vibration modes	578:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	2	59	theme	Raman	445:449	arg1	spectroscopy					451:462	Raman spectroscopy	445:462	Raman spectroscopy	445:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	3	60	theme	X-ray	601:605	arg1	XPS					635:637	XPS	635:637	XPS	635:637	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	3	60	theme	X-ray	601:605	arg1	spectroscopy					621:632	X-ray photoelectron spectroscopy	601:632	X-ray photoelectron spectroscopy (XPS)	601:638	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	3	60	theme	X-ray	601:605	arg1	method					669:674	a quantitative analytical method	643:674	a quantitative analytical method	643:674	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	5	61	theme	surface	933:939	arg1	characterization					952:967	the surface topography characterization	929:967	the surface topography characterization	929:967	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	3	62	theme	surface	789:795	arg1	sensitivity					797:807	high surface sensitivity	784:807	high surface sensitivity	784:807	X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity.					
32858980	2	63	from	identification	512:525	arg1	molecules					559:567	organic molecules	551:567	organic molecules based on their vibration modes	551:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	1	64	dep	temperatures	207:218	arg1	temperatures					207:218	different gelation temperatures	188:218	different gelation temperatures (80 °C and 90 °C)	188:236	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	64	dep	temperatures	207:218	arg1	°C					224:225	80 °C and 90 °C	221:235	°C	224:225	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	1	64	dep	temperatures	207:218	arg1	°C					234:235	80 °C and 90 °C	221:235	°C	234:235	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	5	65	theme	scanning	1032:1039	arg1	SEM					1062:1064	SEM	1062:1064	SEM	1062:1064	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	5	65	theme	scanning	1032:1039	arg1	microscopy					1050:1059	scanning electron microscopy	1032:1059	scanning electron microscopy (SEM)	1032:1065	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	5	66	theme	topography	941:950	arg1	characterization					952:967	the surface topography characterization	929:967	the surface topography characterization	929:967	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	6	67	theme	desired	1327:1333	arg1	characteristics					1335:1349	desired characteristics	1327:1349	desired characteristics	1327:1349	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	7	68	theme	polymer	1516:1522	arg1	sample					1524:1529	the 1,3-β-d-glucan polymer sample	1497:1529	the 1,3-β-d-glucan polymer sample gelled at 80 °C	1497:1545	This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.					
32858980	4	69	theme	Contact	810:816	arg1	goniometer					824:833	Contact angle goniometer	810:833	Contact angle goniometer	810:833	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	1	70	from	effect	178:183	arg1	arrangements					256:267	the structural arrangements	241:267	the structural arrangements in 1,3-β-d-glucan (curdlan) matrices	241:304	In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen.					
32858980	4	71	theme	angle	818:822	arg1	goniometer					824:833	Contact angle goniometer	810:833	Contact angle goniometer	810:833	Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices.					
32858980	0	72	theme	Physicochemical	62:76	arg1	Properties					78:87	Physicochemical Properties	62:87	Physicochemical Properties	62:87	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	6	73	theme	biomaterials	1437:1448	arg1	production					1423:1432	production	1423:1432	production of biomaterials for tissue regeneration	1423:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	73	theme	biomaterials	1437:1448	arg1	entrapment					1400:1409	entrapment	1400:1409	entrapment of drugs	1400:1418	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	2	74	dep	Fourier	389:395	arg1	transform					397:405	transform	397:405	transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy	397:462	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	0	75	dep	Analyses	142:149	arg1	Effect					0:5	Effect	0:5	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix	0:109	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	2	76	theme	groups	541:546	arg1	identification					512:525	the identification	508:525	the identification of functional groups in organic molecules based on their vibration modes	508:598	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	5	77	theme	microscopy	1011:1020	arg1	use					991:993	the use	987:993	the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM)	987:1065	In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM).					
32858980	6	78	with	biomaterials	1309:1320	arg1	characteristics					1335:1349	desired characteristics	1327:1349	desired characteristics	1327:1349	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	78	with	biomaterials	1309:1320	arg1	range					1360:1364	wide range	1355:1364	wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1355:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	2	79	theme	ATR	430:432	arg1	spectroscopy					416:427	spectroscopy	416:427	spectroscopy (ATR FT-IR)	416:439	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	2	79	theme	ATR	430:432	arg1	FT-IR					434:438	ATR FT-IR	430:438	ATR FT-IR	430:438	Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes.					
32858980	0	80	from	Effect	0:5	arg1	Properties					78:87	Physicochemical Properties	62:87	Physicochemical Properties	62:87	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	0	80	from	Effect	0:5	arg1	Structure					48:56	Molecular Structure	38:56	Molecular Structure	38:56	Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses.					
32858980	6	81	theme	matrices	1284:1291	arg1	processes					1164:1172	manufacturing processes	1150:1172	manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration	1150:1472	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
32858980	6	82	theme	biomedical	1369:1378	arg1	applications					1380:1391	biomedical applications	1369:1391	biomedical applications	1369:1391	Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration.					
33341839	0	0	theme	soluble	66:72	arg1	sCD14					84:88	sCD14	84:88	sCD14	84:88	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	0	0	theme	soluble	66:72	arg1	receptor					74:81	its soluble receptor	62:81	its soluble receptor (sCD14) in Saudis	62:99	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	0	1	from	levels	16:21	arg1	Saudis					94:99	Saudis	94:99	Saudis	94:99	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	11	2	theme	endotoxaemia	1633:1644	arg1	CONCLUSIONS					1549:1559	CONCLUSIONS	1549:1559	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia	1549:1644	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	11	2	theme	endotoxaemia	1633:1644	arg1	development					1608:1618	development	1608:1618	development of bacterial endotoxaemia	1608:1644	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	11	2	theme	endotoxaemia	1633:1644	arg1	association					1564:1574	An association	1561:1574	An association between dietary composition	1561:1602	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	11	3	dep	CONCLUSIONS	1549:1559	arg1	CONCLUSIONS					1549:1559	CONCLUSIONS	1549:1559	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia	1549:1644	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	11	3	dep	CONCLUSIONS	1549:1559	arg1	development					1608:1618	development	1608:1618	development of bacterial endotoxaemia	1608:1644	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	11	3	dep	CONCLUSIONS	1549:1559	arg1	association					1564:1574	An association	1561:1574	An association between dietary composition	1561:1602	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	7	4	theme	LDL-C	1009:1013	arg1	Means					996:1000	Means	996:1000	Means of TG, LDL-C, and hs-CRP	996:1025	RESULTS Means of TG, LDL-C, and hs-CRP increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both).					
33341839	6	5	used	used	915:918	arg2	Chi-square					860:869	Chi-square	860:869	Chi-square	860:869	Chi-square, and Pearson's correlation coefficient were used to identify association and correlations between studied variables.					
33341839	6	5	used	used	915:918	arg2	coefficient					898:908	Pearson's correlation coefficient	876:908	Pearson's correlation coefficient	876:908	Chi-square, and Pearson's correlation coefficient were used to identify association and correlations between studied variables.					
33341839	7	6	theme	TG	1005:1006	arg1	Means					996:1000	Means	996:1000	Means of TG, LDL-C, and hs-CRP	996:1025	RESULTS Means of TG, LDL-C, and hs-CRP increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both).					
33341839	12	7	theme	endotoxaemia	1701:1712	arg1	adiposity					1728:1736	adiposity	1728:1736	adiposity	1728:1736	However, no association between measures of endotoxaemia and increased adiposity and inflammation was found.					
33341839	12	7	theme	endotoxaemia	1701:1712	arg1	measures					1689:1696	measures	1689:1696	measures of endotoxaemia	1689:1712	However, no association between measures of endotoxaemia and increased adiposity and inflammation was found.					
33341839	12	7	theme	endotoxaemia	1701:1712	arg1	inflammation					1742:1753	inflammation	1742:1753	inflammation	1742:1753	However, no association between measures of endotoxaemia and increased adiposity and inflammation was found.					
33341839	1	8	dep	OBJECTIVE	102:110	arg1	aimed					123:127	aimed	123:127	aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia	123:304	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	7	9	theme	hs-CRP	1020:1025	arg1	Means					996:1000	Means	996:1000	Means of TG, LDL-C, and hs-CRP	996:1025	RESULTS Means of TG, LDL-C, and hs-CRP increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both).					
33341839	3	10	theme	standardized	489:500	arg1	techniques					502:511	standardized techniques	489:511	standardized techniques	489:511	Anthropometric measurements, and vital signs were taken using standardized techniques.					
33341839	11	11	theme	bacterial	1623:1631	arg1	endotoxaemia					1633:1644	bacterial endotoxaemia	1623:1644	bacterial endotoxaemia	1623:1644	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	2	12	dep	METHODS	307:313	arg1	adults					334:339	Apparently healthy adults	315:339	Apparently healthy adults	315:339	METHODS Apparently healthy adults, aged 18-55 years, were recruited from Jeddah population in a cross-sectional design.					
33341839	5	13	theme	estimated	744:752	arg1	variables					754:762	estimated variables	744:762	estimated variables	744:762	Means of estimated variables were compared using t-test or Mann-Whitney U-test for two groups and ANOVA for multiple groups.					
33341839	6	14	theme	studied	969:975	arg1	variables					977:985	studied variables	969:985	studied variables	969:985	Chi-square, and Pearson's correlation coefficient were used to identify association and correlations between studied variables.					
33341839	0	15	theme	lipopolysaccharide	33:50	arg1	levels					16:21	levels	16:21	levels of plasma lipopolysaccharide (LPS)	16:56	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	0	15	theme	lipopolysaccharide	33:50	arg1	sCD14					84:88	sCD14	84:88	sCD14	84:88	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	0	15	theme	lipopolysaccharide	33:50	arg1	receptor					74:81	its soluble receptor	62:81	its soluble receptor (sCD14) in Saudis	62:99	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	4	16	theme	lipoprotein-	580:591	arg1	cholesterol					593:603	high-density lipoprotein- cholesterol	567:603	high-density lipoprotein- cholesterol (HDL-C)	567:611	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	4	16	theme	lipoprotein-	580:591	arg1	HDL-C					606:610	HDL-C	606:610	HDL-C	606:610	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	2	17	theme	Jeddah	380:385	arg1	population					387:396	Jeddah population	380:396	Jeddah population	380:396	METHODS Apparently healthy adults, aged 18-55 years, were recruited from Jeddah population in a cross-sectional design.					
33341839	4	18	theme	Serum	514:518	arg1	glucose					520:526	Serum glucose	514:526	Serum glucose	514:526	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	9	19	theme	bread	1448:1452	arg1	quantity					1436:1443	high quantity	1431:1443	high quantity of bread (p= 0.033) or drinking tea (p = 0.018)	1431:1491	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	8	20	dep	identified	1323:1332	arg1	0.001					1305:1309	0.001	1305:1309	0.001	1305:1309	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	8	20	dep	identified	1323:1332	arg1	p=0.025					1284:1290	p=0.025	1284:1290	p=0.025	1284:1290	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	2	21	theme	healthy	326:332	arg1	adults					334:339	Apparently healthy adults	315:339	Apparently healthy adults	315:339	METHODS Apparently healthy adults, aged 18-55 years, were recruited from Jeddah population in a cross-sectional design.					
33341839	1	22	theme	obesity	222:228	arg1	levels					174:179	serum levels	168:179	serum levels of LPS, sCD14 and hs-CRP	168:204	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	22	theme	obesity	222:228	arg1	markers					211:217	markers	211:217	markers of obesity	211:228	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	22	theme	obesity	222:228	arg1	composition					243:253	dietary composition	235:253	dietary composition	235:253	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	0	23	from	receptor	74:81	arg1	Saudis					94:99	Saudis	94:99	Saudis	94:99	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	8	24	contain	have	1337:1340	arg2	p<0.001					1362:1368	p<0.001	1362:1368	p<0.001	1362:1368	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	8	24	contain	have	1337:1340	arg1	hs-CRP					1216:1221	Mean hs-CRP	1211:1221	Mean hs-CRP	1211:1221	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	8	24	contain	have	1337:1340	arg2	syndrome					1352:1359	metabolic syndrome	1342:1359	metabolic syndrome (p<0.001)	1342:1369	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	5	25	theme	variables	754:762	arg1	Means					735:739	Means	735:739	Means of estimated variables	735:762	Means of estimated variables were compared using t-test or Mann-Whitney U-test for two groups and ANOVA for multiple groups.					
33341839	8	26	theme	metabolic	1342:1350	arg1	p<0.001					1362:1368	p<0.001	1362:1368	p<0.001	1362:1368	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	8	26	theme	metabolic	1342:1350	arg1	syndrome					1352:1359	metabolic syndrome	1342:1359	metabolic syndrome (p<0.001)	1342:1369	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	9	27	theme	tea	1477:1479	arg1	quantity					1436:1443	high quantity	1431:1443	high quantity of bread (p= 0.033) or drinking tea (p = 0.018)	1431:1491	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	9	28	theme	drinking	1468:1475	arg1	tea					1477:1479	drinking tea	1468:1479	drinking tea (p = 0.018)	1468:1491	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	9	28	theme	drinking	1468:1475	arg1	0.018					1486:1490	p = 0.018	1482:1490	p = 0.018	1482:1490	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	4	29	theme	LDL-	654:657	arg1	LDL-C					672:676	LDL-C	672:676	LDL-C	672:676	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	4	29	theme	LDL-	654:657	arg1	cholesterol					659:669	LDL- cholesterol	654:669	LDL- cholesterol (LDL-C)	654:677	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	1	30	theme	serum	168:172	arg1	levels					174:179	serum levels	168:179	serum levels of LPS, sCD14 and hs-CRP	168:204	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	2	31	theme	cross-sectional	403:417	arg1	design					419:424	a cross-sectional design	401:424	a cross-sectional design	401:424	METHODS Apparently healthy adults, aged 18-55 years, were recruited from Jeddah population in a cross-sectional design.					
33341839	1	32	theme	dietary	235:241	arg1	composition					243:253	dietary composition	235:253	dietary composition	235:253	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	9	33	theme	p	1482:1482	arg1	tea					1477:1479	drinking tea	1468:1479	drinking tea (p = 0.018)	1468:1491	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	9	33	theme	p	1482:1482	arg1	0.018					1486:1490	p = 0.018	1482:1490	p = 0.018	1482:1490	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	8	34	theme	abdominal	1265:1273	arg1	obesity					1275:1281	abdominal obesity	1265:1281	abdominal obesity	1265:1281	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	4	35	theme	plasma	705:710	arg1	index					696:700	atherogenic index	684:700	atherogenic index of plasma (AIP)	684:716	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	4	35	theme	plasma	705:710	arg1	LDL-C					672:676	LDL-C	672:676	LDL-C	672:676	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	4	35	theme	plasma	705:710	arg1	cholesterol					659:669	LDL- cholesterol	654:669	LDL- cholesterol (LDL-C)	654:677	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	4	36	theme	high-density	567:578	arg1	cholesterol					593:603	high-density lipoprotein- cholesterol	567:603	high-density lipoprotein- cholesterol (HDL-C)	567:611	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	4	36	theme	high-density	567:578	arg1	HDL-C					606:610	HDL-C	606:610	HDL-C	606:610	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	0	37	theme	plasma	26:31	arg1	lipopolysaccharide					33:50	plasma lipopolysaccharide	26:50	plasma lipopolysaccharide (LPS)	26:56	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	0	37	theme	plasma	26:31	arg1	LPS					53:55	LPS	53:55	LPS	53:55	Diet influences levels of plasma lipopolysaccharide (LPS) and its soluble receptor (sCD14) in Saudis.					
33341839	11	38	theme	dietary	1584:1590	arg1	composition					1592:1602	dietary composition	1584:1602	dietary composition	1584:1602	CONCLUSIONS An association between dietary composition and development of bacterial endotoxaemia was found.					
33341839	9	39	theme	=	1484:1484	arg1	tea					1477:1479	drinking tea	1468:1479	drinking tea (p = 0.018)	1468:1491	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	9	39	theme	=	1484:1484	arg1	0.018					1486:1490	p = 0.018	1482:1490	p = 0.018	1482:1490	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	8	40	theme	Mean	1211:1214	arg1	hs-CRP					1216:1221	Mean hs-CRP	1211:1221	Mean hs-CRP	1211:1221	Mean hs-CRP was significantly higher in subjects with abdominal obesity (p=0.025 for men, and 0.001 for women), identified to have metabolic syndrome (p<0.001).					
33341839	1	41	theme	LPS	184:186	arg1	levels					174:179	serum levels	168:179	serum levels of LPS, sCD14 and hs-CRP	168:204	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	41	theme	LPS	184:186	arg1	markers					211:217	markers	211:217	markers of obesity	211:228	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	41	theme	LPS	184:186	arg1	composition					243:253	dietary composition	235:253	dietary composition	235:253	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	7	42	dep	RESULTS	988:994	arg1	increased					1027:1035	increased	1027:1035	increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both)	1027:1208	RESULTS Means of TG, LDL-C, and hs-CRP increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both).					
33341839	5	43	theme	multiple	843:850	arg1	groups					852:857	multiple groups	843:857	multiple groups	843:857	Means of estimated variables were compared using t-test or Mann-Whitney U-test for two groups and ANOVA for multiple groups.					
33341839	7	44	from	LDL-C	1137:1141	arg1	females					1156:1162	females	1156:1162	females	1156:1162	RESULTS Means of TG, LDL-C, and hs-CRP increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both).					
33341839	7	44	from	LDL-C	1137:1141	arg1	males					1146:1150	males	1146:1150	males	1146:1150	RESULTS Means of TG, LDL-C, and hs-CRP increased significantly in both genders with increasing BMI (p= 0.019, and 0.040 for TG, 0.049, and 0.002 for LDL-C in males and females respectively, and <0.001 for hs-CRP for both).					
33341839	3	45	theme	Anthropometric	427:440	arg1	measurements					442:453	Anthropometric measurements	427:453	Anthropometric measurements	427:453	Anthropometric measurements, and vital signs were taken using standardized techniques.					
33341839	1	46	theme	healthy	258:264	arg1	adults					266:271	healthy adults	258:271	healthy adults residing in Jeddah, Saudi Arabia	258:304	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	5	47	theme	Mann-Whitney	794:805	arg1	U-test					807:812	Mann-Whitney U-test	794:812	Mann-Whitney U-test	794:812	Means of estimated variables were compared using t-test or Mann-Whitney U-test for two groups and ANOVA for multiple groups.					
33341839	1	48	theme	adults	266:271	arg1	association					148:158	the association	144:158	the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia	144:304	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	9	49	theme	Mean	1372:1375	arg1	sCD14					1377:1381	Mean sCD14	1372:1381	Mean sCD14	1372:1381	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	10	50	from	sCD14	1525:1529	arg1	men					1534:1536	men	1534:1536	men (p=0.049)	1534:1546	LPS correlated positively with sCD14 in men (p=0.049).					
33341839	10	50	from	sCD14	1525:1529	arg1	p=0.049					1539:1545	p=0.049	1539:1545	p=0.049	1539:1545	LPS correlated positively with sCD14 in men (p=0.049).					
33341839	1	51	theme	sCD14	189:193	arg1	levels					174:179	serum levels	168:179	serum levels of LPS, sCD14 and hs-CRP	168:204	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	51	theme	sCD14	189:193	arg1	markers					211:217	markers	211:217	markers of obesity	211:228	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	51	theme	sCD14	189:193	arg1	composition					243:253	dietary composition	235:253	dietary composition	235:253	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	2	52	theme	aged	342:345	arg1	adults					334:339	Apparently healthy adults	315:339	Apparently healthy adults	315:339	METHODS Apparently healthy adults, aged 18-55 years, were recruited from Jeddah population in a cross-sectional design.					
33341839	9	53	theme	high	1431:1434	arg1	quantity					1436:1443	high quantity	1431:1443	high quantity of bread (p= 0.033) or drinking tea (p = 0.018)	1431:1491	Mean sCD14 was significantly elevated in females consuming high quantity of bread (p= 0.033) or drinking tea (p = 0.018).					
33341839	3	54	theme	vital	460:464	arg1	signs					466:470	vital signs	460:470	vital signs	460:470	Anthropometric measurements, and vital signs were taken using standardized techniques.					
33341839	6	55	theme	correlation	886:896	arg1	coefficient					898:908	Pearson's correlation coefficient	876:908	Pearson's correlation coefficient	876:908	Chi-square, and Pearson's correlation coefficient were used to identify association and correlations between studied variables.					
33341839	4	56	theme	atherogenic	684:694	arg1	index					696:700	atherogenic index	684:700	atherogenic index of plasma (AIP)	684:716	Serum glucose, cholesterol (TC), triglycerides (TG), high-density lipoprotein- cholesterol (HDL-C), hs-CPR, LPS and sCD14 were assayed, and LDL- cholesterol (LDL-C), and atherogenic index of plasma (AIP) were calculated.					
33341839	12	57	theme	increased	1718:1726	arg1	adiposity					1728:1736	adiposity	1728:1736	adiposity	1728:1736	However, no association between measures of endotoxaemia and increased adiposity and inflammation was found.					
33341839	1	58	theme	hs-CRP	199:204	arg1	levels					174:179	serum levels	168:179	serum levels of LPS, sCD14 and hs-CRP	168:204	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	58	theme	hs-CRP	199:204	arg1	markers					211:217	markers	211:217	markers of obesity	211:228	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
33341839	1	58	theme	hs-CRP	199:204	arg1	composition					243:253	dietary composition	235:253	dietary composition	235:253	OBJECTIVE This study aimed to investigate the association between serum levels of LPS, sCD14 and hs-CRP, and markers of obesity, and dietary composition of healthy adults residing in Jeddah, Saudi Arabia.					
32222519	0	0	theme	Cu	101:102	arg1	recovery					89:96	selective recovery	79:96	selective recovery of Cu(Ⅱ) from wastewater	79:121	Preparation of ion-imprinted montmorillonite nanosheets/chitosan gel beads for selective recovery of Cu(Ⅱ) from wastewater.					
32222519	1	1	theme	nanosheets/chitosan	164:182	arg1	beads					196:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	5	2	theme	NaOH	1120:1123	arg1	solution					1125:1132	NaOH solution	1120:1132	NaOH solution	1120:1132	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	3	3	theme	honeycombed	489:499	arg1	pores					501:505	honeycombed pores	489:505	honeycombed pores	489:505	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	4	4	theme	high	863:866	arg1	performance					868:878	its high performance	859:878	its high performance of cation exchange	859:897	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	2	5	theme	scanning	292:299	arg1	Fourier					328:334	Fourier	328:334	Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	328:417	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	5	theme	scanning	292:299	arg1	microscope					310:319	scanning electron microscope	292:319	scanning electron microscope (SEM)	292:325	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	5	theme	scanning	292:299	arg1	SEM					322:324	SEM	322:324	SEM	322:324	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	6	dep	Fourier	328:334	arg1	transform					336:344	transform	336:344	transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	336:417	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	5	7	theme	MMTNS	1080:1084	arg1	MMTNS					1080:1084	MMTNS	1080:1084	MMTNS	1080:1084	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	5	7	theme	MMTNS	1080:1084	arg1	sites					1071:1075	the adsorption sites	1056:1075	the adsorption sites of MMTNS	1056:1084	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	3	8	from	pores	501:505	arg1	rich					481:484	rich	481:484	rich	481:484	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	6	9	theme	various	1250:1256	arg1	cations					1258:1264	various cations	1250:1264	various cations	1250:1264	More importantly, its high selectivity for Cu2+ provides a feasible way to recover Cu2+ from wastewater containing various cations.					
32222519	6	10	theme	high	1157:1160	arg1	selectivity					1162:1172	its high selectivity	1153:1172	its high selectivity for Cu2+	1153:1181	More importantly, its high selectivity for Cu2+ provides a feasible way to recover Cu2+ from wastewater containing various cations.					
32222519	1	11	theme	IIMNC	185:189	arg1	beads					196:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	4	12	theme	adsorption	834:843	arg1	sites					845:849	active adsorption sites	827:849	active adsorption sites	827:849	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	1	13	theme	gel	192:194	arg1	beads					196:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	4	14	theme	active	827:832	arg1	sites					845:849	active adsorption sites	827:849	active adsorption sites	827:849	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	4	15	theme	copper	646:651	arg1	elimination					631:641	The elimination	627:641	The elimination of copper	627:651	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	3	16	theme	Cu2+	547:550	arg1	removal					536:542	the removal	532:542	the removal of Cu2+	532:550	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	0	17	theme	montmorillonite	29:43	arg1	Preparation					0:10	Preparation	0:10	Preparation of ion-imprinted montmorillonite	0:43	Preparation of ion-imprinted montmorillonite nanosheets/chitosan gel beads for selective recovery of Cu(Ⅱ) from wastewater.					
32222519	4	18	theme	montmorillonite	726:740	arg1	MMTNS					754:758	MMTNS	754:758	MMTNS	754:758	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	4	18	theme	montmorillonite	726:740	arg1	nanosheets					742:751	introduced montmorillonite nanosheets	715:751	introduced montmorillonite nanosheets (MMTNS)	715:759	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	3	19	from	rich	481:484	arg1	pores					501:505	honeycombed pores	489:505	honeycombed pores	489:505	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	2	20	dep	transform	336:344	arg1	infrared					346:353	infrared	346:353	transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	336:417	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	0	21	theme	ion-imprinted	15:27	arg1	montmorillonite					29:43	ion-imprinted montmorillonite	15:43	ion-imprinted montmorillonite	15:43	Preparation of ion-imprinted montmorillonite nanosheets/chitosan gel beads for selective recovery of Cu(Ⅱ) from wastewater.					
32222519	2	22	theme	electron	301:308	arg1	Fourier					328:334	Fourier	328:334	Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)	328:417	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	22	theme	electron	301:308	arg1	microscope					310:319	scanning electron microscope	292:319	scanning electron microscope (SEM)	292:325	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	22	theme	electron	301:308	arg1	SEM					322:324	SEM	322:324	SEM	322:324	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	6	23	contain	containing	1239:1248	arg1	wastewater					1228:1237	wastewater	1228:1237	wastewater containing various cations	1228:1264	More importantly, its high selectivity for Cu2+ provides a feasible way to recover Cu2+ from wastewater containing various cations.					
32222519	6	23	contain	containing	1239:1248	arg2	cations					1258:1264	various cations	1250:1264	various cations	1250:1264	More importantly, its high selectivity for Cu2+ provides a feasible way to recover Cu2+ from wastewater containing various cations.					
32222519	4	24	theme	pseudo-second-order	669:687	arg1	model					689:693	pseudo-second-order model	669:693	pseudo-second-order model	669:693	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	0	25	theme	gel	65:67	arg1	beads					69:73	gel beads	65:73	gel beads	65:73	Preparation of ion-imprinted montmorillonite nanosheets/chitosan gel beads for selective recovery of Cu(Ⅱ) from wastewater.					
32222519	5	26	theme	adsorption	1060:1069	arg1	MMTNS					1080:1084	MMTNS	1080:1084	MMTNS	1080:1084	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	5	26	theme	adsorption	1060:1069	arg1	sites					1071:1075	the adsorption sites	1056:1075	the adsorption sites of MMTNS	1056:1084	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	3	27	theme	synergistic	564:574	arg1	effect					576:581	the synergistic effect	560:581	the synergistic effect of montmorillonite nanosheets	560:611	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	2	28	theme	X-ray	380:384	arg1	spectroscopy					400:411	X-ray photoelectron spectroscopy	380:411	X-ray photoelectron spectroscopy (XPS)	380:417	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	28	theme	X-ray	380:384	arg1	XPS					414:416	XPS	414:416	XPS	414:416	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	1	29	theme	selective	220:228	arg1	adsorption					230:239	selective adsorption	220:239	selective adsorption of Cu2+	220:247	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	4	30	theme	negative	798:805	arg1	charge					807:812	its negative charge	794:812	its negative charge	794:812	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	5	31	theme	Cu2+	988:991	arg1	removal					977:983	the removal	973:983	the removal of Cu2+	973:991	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	4	32	theme	exchange	890:897	arg1	performance					868:878	its high performance	859:878	its high performance of cation exchange	859:897	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	6	33	theme	feasible	1194:1201	arg1	way					1203:1205	a feasible way	1192:1205	a feasible way to recover Cu2+ from wastewater containing various cations	1192:1264	More importantly, its high selectivity for Cu2+ provides a feasible way to recover Cu2+ from wastewater containing various cations.					
32222519	3	34	theme	nanosheets	602:611	arg1	chitosan					617:624	chitosan	617:624	chitosan	617:624	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	3	34	theme	nanosheets	602:611	arg1	effect					576:581	the synergistic effect	560:581	the synergistic effect of montmorillonite nanosheets	560:611	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	4	35	theme	introduced	715:724	arg1	MMTNS					754:758	MMTNS	754:758	MMTNS	754:758	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	4	35	theme	introduced	715:724	arg1	nanosheets					742:751	introduced montmorillonite nanosheets	715:751	introduced montmorillonite nanosheets (MMTNS)	715:759	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	5	36	theme	excellent	931:939	arg1	reusability					941:951	excellent reusability	931:951	excellent reusability	931:951	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	4	37	theme	cation	883:888	arg1	exchange					890:897	cation exchange	883:897	cation exchange	883:897	The elimination of copper was followed by pseudo-second-order model and was enhanced by introduced montmorillonite nanosheets (MMTNS) because MMTNS attracted Cu(Ⅱ) by its negative charge and provided active adsorption sites through its high performance of cation exchange.					
32222519	2	38	theme	gel	260:262	arg1	beads					264:268	The IIMNC gel beads	250:268	The IIMNC gel beads	250:268	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	1	39	theme	novel	128:132	arg1	beads					196:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	5	40	theme	composite	905:913	arg1	gel					915:917	This composite gel	900:917	This composite gel	900:917	This composite gel also showed excellent reusability, performing well in the removal of Cu2+ after undergoing adsorption-desorption in five cycles, because the adsorption sites of MMTNS can be continually reactivated by NaOH solution.					
32222519	2	41	theme	photoelectron	386:398	arg1	spectroscopy					400:411	X-ray photoelectron spectroscopy	380:411	X-ray photoelectron spectroscopy (XPS)	380:417	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	41	theme	photoelectron	386:398	arg1	XPS					414:416	XPS	414:416	XPS	414:416	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	2	42	theme	IIMNC	254:258	arg1	beads					264:268	The IIMNC gel beads	250:268	The IIMNC gel beads	250:268	The IIMNC gel beads were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).					
32222519	1	43	theme	ion-imprinted	134:146	arg1	beads					196:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	1	44	theme	Cu2+	244:247	arg1	adsorption					230:239	selective adsorption	220:239	selective adsorption of Cu2+	220:247	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	0	45	theme	selective	79:87	arg1	recovery					89:96	selective recovery	79:96	selective recovery of Cu(Ⅱ) from wastewater	79:121	Preparation of ion-imprinted montmorillonite nanosheets/chitosan gel beads for selective recovery of Cu(Ⅱ) from wastewater.					
32222519	3	46	theme	montmorillonite	586:600	arg1	nanosheets					602:611	montmorillonite nanosheets	586:611	montmorillonite nanosheets	586:611	The results showed that IIMNC was successfully assembled and rich in honeycombed pores, which performed well in the removal of Cu2+ through the synergistic effect of montmorillonite nanosheets and chitosan.					
32222519	1	47	theme	montmorillonite	148:162	arg1	beads					196:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads	124:200	The novel ion-imprinted montmorillonite nanosheets/chitosan (IIMNC) gel beads were prepared for selective adsorption of Cu2+.					
32222519	0	48	from	wastewater	112:121	arg1	recovery					89:96	selective recovery	79:96	selective recovery of Cu(Ⅱ) from wastewater	79:121	Preparation of ion-imprinted montmorillonite nanosheets/chitosan gel beads for selective recovery of Cu(Ⅱ) from wastewater.					
31935409	2	0	theme	different	472:480	arg1	concentrations					482:495	different concentrations	472:495	different concentrations of HS	472:501	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	1	with	scaffolds	384:392	arg1	HS					423:424	HS	423:424	HS	423:424	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	1	with	scaffolds	384:392	arg1	sulfate					414:420	loaded heparan sulfate	399:420	loaded heparan sulfate (HS)	399:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	4	2	theme	in	668:669	arg1	experiments					676:686	both in vitro and in vivo experiments	650:686	both in vitro and in vivo experiments	650:686	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	1	3	theme	biomedical	168:177	arg1	field					179:183	biomedical field	168:183	biomedical field	168:183	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	0	4	theme	defect	91:96	arg1	repair					98:103	bone defect repair	86:103	bone defect repair	86:103	Heparan sulfate loaded polycaprolactone-hydroxyapatite scaffolds with 3D printing for bone defect repair.					
31935409	5	5	theme	HS	873:874	arg1	concentration					856:868	high concentration	851:868	high concentration of HS	851:874	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	5	6	theme	positive	973:980	arg1	effects					982:988	positive effects	973:988	positive effects	973:988	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	4	7	theme	positive	738:745	arg1	effects					747:753	positive effects	738:753	positive effects in promoting osteoblast maturation and accelerating bone defect repair	738:824	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	2	8	theme	current	293:299	arg1	study					301:305	the current study	289:305	the current study	289:305	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	4	9	theme	low	707:709	arg1	concentration					711:723	low concentration	707:723	low concentration of HS	707:729	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	1	10	theme	next	268:271	arg1	development					273:283	the next development	264:283	the next development	264:283	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	6	11	theme	defect	1253:1258	arg1	repair					1260:1265	bone defect repair	1248:1265	bone defect repair	1248:1265	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	6	12	theme	bone	1188:1191	arg1	defects					1193:1199	biological bone defects	1177:1199	biological bone defects	1177:1199	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	2	13	theme	PCL-HA	369:374	arg1	scaffolds					384:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds	325:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS)	325:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	5	14	from	defects	1008:1014	arg1	organisms					1019:1027	organisms	1019:1027	organisms	1019:1027	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	6	15	theme	PCL-HA-HS	1101:1109	arg1	scaffold					1121:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	4	16	theme	defect	812:817	arg1	repair					819:824	bone defect repair	807:824	bone defect repair	807:824	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	6	17	theme	good	1041:1044	arg1	properties					1057:1066	good structural properties	1041:1066	good structural properties	1041:1066	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	5	18	dep	showed	876:881	arg1	brought					959:965	brought	959:965	brought about positive effects in repairing bone defects in organisms	959:1027	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	5	19	theme	inhibitive	891:900	arg1	effort					902:907	notable inhibitive effort	883:907	notable inhibitive effort	883:907	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	2	20	theme	heparan	406:412	arg1	HS					423:424	HS	423:424	HS	423:424	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	20	theme	heparan	406:412	arg1	sulfate					414:420	loaded heparan sulfate	399:420	loaded heparan sulfate (HS)	399:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	21	theme	HS	500:501	arg1	concentrations					482:495	different concentrations	472:495	different concentrations of HS	472:501	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	22	from	effect	462:467	arg1	healing					510:516	the healing	506:516	the healing of bone defects	506:532	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	4	23	theme	bone	807:810	arg1	repair					819:824	bone defect repair	807:824	bone defect repair	807:824	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	4	24	dep	in	655:656	arg1	vitro					658:662	vitro	658:662	vitro	658:662	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	6	25	theme	structural	1046:1055	arg1	properties					1057:1066	good structural properties	1041:1066	good structural properties	1041:1066	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	2	26	theme	loaded	399:404	arg1	HS					423:424	HS	423:424	HS	423:424	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	26	theme	loaded	399:404	arg1	sulfate					414:420	loaded heparan sulfate	399:420	loaded heparan sulfate (HS)	399:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	6	27	theme	printed	1093:1099	arg1	scaffold					1121:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	0	28	theme	Heparan	0:6	arg1	sulfate					8:14	Heparan sulfate	0:14	Heparan sulfate	0:14	Heparan sulfate loaded polycaprolactone-hydroxyapatite scaffolds with 3D printing for bone defect repair.					
31935409	6	29	theme	effective	1222:1230	arg1	biomaterial					1232:1242	an effective biomaterial	1219:1242	an effective biomaterial for bone defect repair	1219:1265	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	1	30	from	applications	126:137	arg1	field					179:183	biomedical field	168:183	biomedical field	168:183	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	6	31	theme	bone	1248:1251	arg1	repair					1260:1265	bone defect repair	1248:1265	bone defect repair	1248:1265	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	0	32	theme	polycaprolactone-hydroxyapatite	23:53	arg1	scaffolds					55:63	polycaprolactone-hydroxyapatite scaffolds	23:63	polycaprolactone-hydroxyapatite scaffolds	23:63	Heparan sulfate loaded polycaprolactone-hydroxyapatite scaffolds with 3D printing for bone defect repair.					
31935409	6	33	theme	biological	1177:1186	arg1	defects					1193:1199	biological bone defects	1177:1199	biological bone defects	1177:1199	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	5	34	theme	osteoblasts	933:943	arg1	proliferation					916:928	the proliferation	912:928	the proliferation of osteoblasts	912:943	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	2	35	theme	defects	526:532	arg1	healing					510:516	the healing	506:516	the healing of bone defects	506:532	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	1	36	theme	increasing	115:124	arg1	applications					126:137	the increasing applications	111:137	the increasing applications of 3D printing technology in biomedical field	111:183	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	1	37	theme	technology	154:163	arg1	applications					126:137	the increasing applications	111:137	the increasing applications of 3D printing technology in biomedical field	111:183	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	5	38	theme	notable	883:889	arg1	effort					902:907	notable inhibitive effort	883:907	notable inhibitive effort	883:907	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	2	39	theme	bone	521:524	arg1	defects					526:532	bone defects	521:532	bone defects	521:532	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	3	40	theme	good	622:625	arg1	resistance					593:602	sound compression resistance	575:602	sound compression resistance	575:602	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	40	theme	good	622:625	arg1	result					540:545	a result	538:545	a result	538:545	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	40	theme	good	622:625	arg1	porosity					609:616	air porosity	605:616	air porosity	605:616	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	40	theme	good	622:625	arg1	biocompatibility					627:642	good biocompatibility	622:642	good biocompatibility	622:642	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	1	41	dep	composition	190:200	arg1	the					186:188	the	186:188	the	186:188	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	2	42	theme	reparative	451:460	arg1	effect					462:467	the reparative effect	447:467	the reparative effect of different concentrations of HS on the healing of bone defects	447:532	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	3	43	theme	air	605:607	arg1	resistance					593:602	sound compression resistance	575:602	sound compression resistance	575:602	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	43	theme	air	605:607	arg1	result					540:545	a result	538:545	a result	538:545	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	43	theme	air	605:607	arg1	porosity					609:616	air porosity	605:616	air porosity	605:616	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	43	theme	air	605:607	arg1	biocompatibility					627:642	good biocompatibility	622:642	good biocompatibility	622:642	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	4	44	theme	osteoblast	768:777	arg1	maturation					779:788	osteoblast maturation	768:788	osteoblast maturation	768:788	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	1	45	theme	related	222:228	arg1	materials					230:238	the related materials	218:238	the related materials	218:238	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	5	46	theme	high	851:854	arg1	concentration					856:868	high concentration	851:868	high concentration of HS	851:874	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	5	47	with	scaffold	837:844	arg1	concentration					856:868	high concentration	851:868	high concentration of HS	851:874	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	6	48	theme	3D	1090:1091	arg1	scaffold					1121:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	2	49	theme	polycaprolactone-hydroxyapatite	336:366	arg1	scaffolds					384:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds	325:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS)	325:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	0	50	theme	3D	70:71	arg1	printing					73:80	3D printing	70:80	3D printing for bone defect repair	70:103	Heparan sulfate loaded polycaprolactone-hydroxyapatite scaffolds with 3D printing for bone defect repair.					
31935409	5	51	theme	bone	1003:1006	arg1	defects					1008:1014	bone defects	1003:1014	bone defects in organisms	1003:1027	Moreover, scaffold with high concentration of HS showed notable inhibitive effort on the proliferation of osteoblasts, yet it still brought about positive effects in repairing bone defects in organisms.					
31935409	2	52	theme	printed	328:334	arg1	scaffolds					384:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds	325:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS)	325:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	4	53	with	scaffold	693:700	arg1	concentration					711:723	low concentration	707:723	low concentration of HS	707:729	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	6	54	theme	defects	1193:1199	arg1	repairing					1164:1172	the repairing	1160:1172	the repairing of biological bone defects	1160:1199	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	1	55	theme	3D	142:143	arg1	technology					154:163	3D printing technology	142:163	3D printing technology	142:163	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	1	56	theme	materials	230:238	arg1	composition					190:200	composition	190:200	composition	190:200	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	1	56	theme	materials	230:238	arg1	additives					205:213	additives	205:213	additives	205:213	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	4	57	theme	HS	728:729	arg1	concentration					711:723	low concentration	707:723	low concentration of HS	707:729	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	1	58	theme	printing	145:152	arg1	technology					154:163	3D printing technology	142:163	3D printing technology	142:163	With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development.					
31935409	2	59	theme	3D	325:326	arg1	scaffolds					384:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds	325:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS)	325:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	0	60	theme	bone	86:89	arg1	repair					98:103	bone defect repair	86:103	bone defect repair	86:103	Heparan sulfate loaded polycaprolactone-hydroxyapatite scaffolds with 3D printing for bone defect repair.					
31935409	3	61	theme	sound	575:579	arg1	result					540:545	a result	538:545	a result	538:545	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	61	theme	sound	575:579	arg1	resistance					593:602	sound compression resistance	575:602	sound compression resistance	575:602	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	61	theme	sound	575:579	arg1	porosity					609:616	air porosity	605:616	air porosity	605:616	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	61	theme	sound	575:579	arg1	biocompatibility					627:642	good biocompatibility	622:642	good biocompatibility	622:642	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	2	62	theme	porous	377:382	arg1	scaffolds					384:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds	325:392	3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS)	325:425	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	2	63	theme	concentrations	482:495	arg1	effect					462:467	the reparative effect	447:467	the reparative effect of different concentrations of HS on the healing of bone defects	447:532	In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects.					
31935409	6	64	theme	composite	1111:1119	arg1	scaffold					1121:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	3D printed PCL-HA-HS composite scaffold	1090:1128	Thus, with good structural properties and biocompatibility, 3D printed PCL-HA-HS composite scaffold can facilitate and accelerate the repairing of biological bone defects, demonstrating as an effective biomaterial for bone defect repair.					
31935409	3	65	theme	compression	581:591	arg1	result					540:545	a result	538:545	a result	538:545	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	65	theme	compression	581:591	arg1	resistance					593:602	sound compression resistance	575:602	sound compression resistance	575:602	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	65	theme	compression	581:591	arg1	porosity					609:616	air porosity	605:616	air porosity	605:616	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	3	65	theme	compression	581:591	arg1	biocompatibility					627:642	good biocompatibility	622:642	good biocompatibility	622:642	As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility.					
31935409	4	66	theme	in	655:656	arg1	experiments					676:686	both in vitro and in vivo experiments	650:686	both in vitro and in vivo experiments	650:686	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
31935409	4	67	dep	in	668:669	arg1	vivo					671:674	vivo	671:674	vivo	671:674	From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation and accelerating bone defect repair.					
32507183	2	0	theme	nano-assemblies	583:597	arg1	biocompatibility					559:574	the biocompatibility	555:574	the biocompatibility of the nano-assemblies	555:597	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	8	1	theme	dual	1696:1699	arg1	capabilities					1701:1712	photothermal/photoacoustic dual capabilities	1669:1712	photothermal/photoacoustic dual capabilities	1669:1712	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	8	2	theme	photothermal/photoacoustic	1669:1694	arg1	capabilities					1701:1712	photothermal/photoacoustic dual capabilities	1669:1712	photothermal/photoacoustic dual capabilities	1669:1712	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	7	3	theme	photoacoustic	1463:1475	arg1	imaging					1477:1483	real-time photoacoustic imaging	1453:1483	real-time photoacoustic imaging	1453:1483	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	8	4	theme	nano-assemblies	1648:1662	arg1	synthesis					1617:1625	the scalable synthesis	1604:1625	the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility	1604:1742	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	8	5	theme	scalable	1608:1615	arg1	synthesis					1617:1625	the scalable synthesis	1604:1625	the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility	1604:1742	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	6	6	theme	nanoscale	1245:1253	arg1	dimensions					1255:1264	controllable nanoscale dimensions	1232:1264	controllable nanoscale dimensions	1232:1264	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	8	7	with	nano-assemblies	1648:1662	arg1	biocompatibility					1727:1742	enhanced biocompatibility	1718:1742	enhanced biocompatibility	1718:1742	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	8	7	with	nano-assemblies	1648:1662	arg1	capabilities					1701:1712	photothermal/photoacoustic dual capabilities	1669:1712	photothermal/photoacoustic dual capabilities	1669:1712	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	8	8	theme	enhanced	1718:1725	arg1	biocompatibility					1727:1742	enhanced biocompatibility	1718:1742	enhanced biocompatibility	1718:1742	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	3	9	theme	Dex-PPy	813:819	arg1	NAs					821:823	Dex-PPy NAs	813:823	Dex-PPy NAs	813:823	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	3	9	theme	Dex-PPy	813:819	arg1	nano-assemblies					796:810	dextran-polypyrrole nano-assemblies	776:810	dextran-polypyrrole nano-assemblies (Dex-PPy NAs)	776:824	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	4	10	theme	monomers	995:1002	arg1	polymerization					969:982	the polymerization	965:982	the polymerization of pyrrole monomers	965:1002	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	7	11	theme	imaging	1477:1483	arg1	capabilities					1510:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities	1443:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors	1443:1542	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	5	12	theme	resultant	1079:1087	arg1	NAs					1097:1099	The resultant Dex-PPy NAs	1075:1099	The resultant Dex-PPy NAs	1075:1099	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	2	13	contain	have	343:346	arg2	stability					358:366	colloidal stability	348:366	colloidal stability	348:366	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	2	13	contain	have	343:346	arg1	nano-assemblies					321:335	polypyrrole-based nano-assemblies	303:335	polypyrrole-based nano-assemblies which have colloidal stability in biological buffers	303:388	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	4	14	theme	molecular	1057:1065	arg1	chains					1067:1072	dextran molecular chains	1049:1072	dextran molecular chains	1049:1072	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	3	15	theme	ambient	833:839	arg1	conditions					841:850	ambient conditions	833:850	ambient conditions	833:850	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	4	16	theme	pyrrole	987:993	arg1	monomers					995:1002	pyrrole monomers	987:1002	pyrrole monomers	987:1002	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	6	17	theme	in	1320:1321	arg1	studies					1328:1334	both in vitro and in vivo studies	1302:1334	both in vitro and in vivo studies owing to their biocompatible dextran "shell"	1302:1379	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	2	18	theme	biological	371:380	arg1	buffers					382:388	biological buffers	371:388	biological buffers	371:388	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	5	19	contain	have	1101:1104	arg2	efficiency					1132:1141	a photothermal conversion efficiency	1106:1141	a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability	1106:1198	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	5	19	contain	have	1101:1104	arg1	NAs					1097:1099	The resultant Dex-PPy NAs	1075:1099	The resultant Dex-PPy NAs	1075:1099	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	6	20	dep	in	1320:1321	arg1	vivo					1323:1326	vivo	1323:1326	vivo	1323:1326	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	6	21	theme	shell	1374:1378	arg1	"					1379:1379	their biocompatible dextran "shell"	1345:1379	their biocompatible dextran "shell"	1345:1379	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	3	22	theme	"	634:634	arg1	synthesis					647:655	a "polymerization/assembly" two-in-one synthesis	608:655	a "polymerization/assembly" two-in-one synthesis	608:655	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	3	23	from	synthesis	712:720	arg1	media					867:871	aqueous media	859:871	aqueous media	859:871	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	2	24	theme	polypyrrole-based	485:501	arg1	copolymers					503:512	polypyrrole-based copolymers	485:512	polypyrrole-based copolymers	485:512	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	6	25	with	NAs	1223:1225	arg1	dimensions					1255:1264	controllable nanoscale dimensions	1232:1264	controllable nanoscale dimensions	1232:1264	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	3	26	theme	two-in-one	636:645	arg1	synthesis					647:655	a "polymerization/assembly" two-in-one synthesis	608:655	a "polymerization/assembly" two-in-one synthesis	608:655	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	1	27	theme	excellent	229:237	arg1	capabilities					263:274	its excellent photothermal conversion capabilities	225:274	its excellent photothermal conversion capabilities	225:274	Polypyrroles have shown great potential in photoacoustic imaging and photothermal therapy owing to its excellent photothermal conversion capabilities.					
32507183	3	28	theme	polymerization/assembly	611:633	arg1	"					634:634	a "polymerization/assembly"	608:634	a "polymerization/assembly" two-in-one synthesis	608:655	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	6	29	theme	dextran	1365:1371	arg1	"					1379:1379	their biocompatible dextran "shell"	1345:1379	their biocompatible dextran "shell"	1345:1379	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	5	30	theme	conversion	1121:1130	arg1	efficiency					1132:1141	a photothermal conversion efficiency	1106:1141	a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability	1106:1198	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	7	31	theme	malignant	1527:1535	arg1	tumors					1537:1542	malignant tumors	1527:1542	malignant tumors	1527:1542	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	1	32	theme	photothermal	239:250	arg1	capabilities					263:274	its excellent photothermal conversion capabilities	225:274	its excellent photothermal conversion capabilities	225:274	Polypyrroles have shown great potential in photoacoustic imaging and photothermal therapy owing to its excellent photothermal conversion capabilities.					
32507183	0	33	theme	Scalable	0:7	arg1	dextran-polypyrrole					9:27	Scalable dextran-polypyrrole	0:27	Scalable dextran-polypyrrole	0:27	Scalable dextran-polypyrrole nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	3	34	theme	aqueous	859:865	arg1	media					867:871	aqueous media	859:871	aqueous media	859:871	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	6	35	theme	biocompatible	1351:1363	arg1	"					1379:1379	their biocompatible dextran "shell"	1345:1379	their biocompatible dextran "shell"	1345:1379	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	3	36	theme	first	677:681	arg1	time					683:686	the first time	673:686	the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media	673:871	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	1	37	theme	conversion	252:261	arg1	capabilities					263:274	its excellent photothermal conversion capabilities	225:274	its excellent photothermal conversion capabilities	225:274	Polypyrroles have shown great potential in photoacoustic imaging and photothermal therapy owing to its excellent photothermal conversion capabilities.					
32507183	2	38	theme	additional	527:536	arg1	step					538:541	even an additional step	519:541	even an additional step	519:541	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	4	39	theme	tetravalent	908:918	arg1	ions					927:930	tetravalent cerium ions	908:930	tetravalent cerium ions	908:930	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	4	39	theme	tetravalent	908:918	arg1	initiators					935:944	initiators	935:944	initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains	935:1072	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	4	40	theme	radicals	1035:1042	arg1	initiation					1016:1025	the initiation	1012:1025	the initiation of free radicals from dextran molecular chains	1012:1072	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	1	41	theme	great	150:154	arg1	potential					156:164	great potential	150:164	great potential	150:164	Polypyrroles have shown great potential in photoacoustic imaging and photothermal therapy owing to its excellent photothermal conversion capabilities.					
32507183	7	42	theme	photothermal	1489:1500	arg1	therapy					1502:1508	photothermal therapy	1489:1508	photothermal therapy	1489:1508	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	6	43	theme	in	1307:1308	arg1	studies					1328:1334	both in vitro and in vivo studies	1302:1334	both in vitro and in vivo studies owing to their biocompatible dextran "shell"	1302:1379	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	4	44	theme	free	1030:1033	arg1	radicals					1035:1042	free radicals	1030:1042	free radicals	1030:1042	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	5	45	theme	excellent	1175:1183	arg1	photostability					1185:1198	an excellent photostability	1172:1198	an excellent photostability	1172:1198	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	1	46	theme	photothermal	195:206	arg1	therapy					208:214	photothermal therapy	195:214	photothermal therapy	195:214	Polypyrroles have shown great potential in photoacoustic imaging and photothermal therapy owing to its excellent photothermal conversion capabilities.					
32507183	0	47	theme	dual	77:80	arg1	capabilities					82:93	photothermal/photoacoustic dual capabilities	50:93	photothermal/photoacoustic dual capabilities	50:93	Scalable dextran-polypyrrole nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	4	48	from	chains	1067:1072	arg1	initiation					1016:1025	the initiation	1012:1025	the initiation of free radicals from dextran molecular chains	1012:1072	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	4	49	theme	dextran	1049:1055	arg1	chains					1067:1072	dextran molecular chains	1049:1072	dextran molecular chains	1049:1072	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	3	50	theme	polypyrrole-based	741:757	arg1	nano-assemblies					759:773	polypyrrole-based nano-assemblies	741:773	polypyrrole-based nano-assemblies	741:773	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	2	51	theme	polypyrrole-based	303:319	arg1	nano-assemblies					321:335	polypyrrole-based nano-assemblies	303:335	polypyrrole-based nano-assemblies which have colloidal stability in biological buffers	303:388	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	6	52	dep	in	1307:1308	arg1	vitro					1310:1314	vitro	1310:1314	vitro	1310:1314	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	0	53	theme	photothermal/photoacoustic	50:75	arg1	capabilities					82:93	photothermal/photoacoustic dual capabilities	50:93	photothermal/photoacoustic dual capabilities	50:93	Scalable dextran-polypyrrole nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	3	54	theme	new	727:729	arg1	family					731:736	a new family	725:736	a new family of polypyrrole-based nano-assemblies	725:773	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	3	54	theme	new	727:729	arg1	nano-assemblies					796:810	dextran-polypyrrole nano-assemblies	776:810	dextran-polypyrrole nano-assemblies (Dex-PPy NAs)	776:824	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	4	55	theme	cerium	920:925	arg1	ions					927:930	tetravalent cerium ions	908:930	tetravalent cerium ions	908:930	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	4	55	theme	cerium	920:925	arg1	initiators					935:944	initiators	935:944	initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains	935:1072	In addition, the approach employs tetravalent cerium ions as initiators which can initiate the polymerization of pyrrole monomers through the initiation of free radicals from dextran molecular chains.					
32507183	2	56	theme	copolymers	503:512	arg1	self-assembly					468:480	self-assembly	468:480	self-assembly of polypyrrole-based copolymers	468:512	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	3	57	theme	family	731:736	arg1	synthesis					712:720	the one-step synthesis	699:720	the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media	699:871	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	5	58	theme	Dex-PPy	1089:1095	arg1	NAs					1097:1099	The resultant Dex-PPy NAs	1075:1099	The resultant Dex-PPy NAs	1075:1099	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	8	59	theme	polypyrrole-based	1630:1646	arg1	nano-assemblies					1648:1662	polypyrrole-based nano-assemblies	1630:1662	polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility	1630:1742	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	7	60	dep	in	1385:1386	arg1	vivo					1388:1391	vivo	1388:1391	vivo	1388:1391	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	3	61	theme	dextran-polypyrrole	776:794	arg1	NAs					821:823	Dex-PPy NAs	813:823	Dex-PPy NAs	813:823	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	3	61	theme	dextran-polypyrrole	776:794	arg1	family					731:736	a new family	725:736	a new family of polypyrrole-based nano-assemblies	725:773	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	3	61	theme	dextran-polypyrrole	776:794	arg1	nano-assemblies					796:810	dextran-polypyrrole nano-assemblies	776:810	dextran-polypyrrole nano-assemblies (Dex-PPy NAs)	776:824	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	1	62	theme	photoacoustic	169:181	arg1	imaging					183:189	photoacoustic imaging	169:189	photoacoustic imaging	169:189	Polypyrroles have shown great potential in photoacoustic imaging and photothermal therapy owing to its excellent photothermal conversion capabilities.					
32507183	7	63	theme	excellent	1443:1451	arg1	capabilities					1510:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities	1443:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors	1443:1542	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	7	64	theme	Dex-PPy	1426:1432	arg1	NAs					1434:1436	the Dex-PPy NAs	1422:1436	the Dex-PPy NAs	1422:1436	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	2	65	theme	colloidal	348:356	arg1	stability					358:366	colloidal stability	348:366	colloidal stability	348:366	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	7	66	theme	therapy	1502:1508	arg1	capabilities					1510:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities	1443:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors	1443:1542	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	6	67	theme	controllable	1232:1243	arg1	dimensions					1255:1264	controllable nanoscale dimensions	1232:1264	controllable nanoscale dimensions	1232:1264	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	6	68	theme	cytotoxicity	1286:1297	arg1	signs					1277:1281	no signs	1274:1281	no signs of cytotoxicity	1274:1297	More importantly, the NAs with controllable nanoscale dimensions display no signs of cytotoxicity in both in vitro and in vivo studies owing to their biocompatible dextran "shell".					
32507183	3	69	theme	nano-assemblies	759:773	arg1	family					731:736	a new family	725:736	a new family of polypyrrole-based nano-assemblies	725:773	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	3	69	theme	nano-assemblies	759:773	arg1	nano-assemblies					796:810	dextran-polypyrrole nano-assemblies	776:810	dextran-polypyrrole nano-assemblies (Dex-PPy NAs)	776:824	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	5	70	theme	photothermal	1108:1119	arg1	efficiency					1132:1141	a photothermal conversion efficiency	1106:1141	a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability	1106:1198	The resultant Dex-PPy NAs have a photothermal conversion efficiency reaching as high as 41 % and an excellent photostability.					
32507183	2	71	theme	steps	411:415	arg1	number					401:406	a number	399:406	a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step	399:541	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	2	71	theme	steps	411:415	arg1	polymerization					432:445	the polymerization	428:445	the polymerization of pyrrole monomers	428:465	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	7	72	contain	have	1438:1441	arg2	capabilities					1510:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities	1443:1521	excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors	1443:1542	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	7	72	contain	have	1438:1441	arg1	NAs					1434:1436	the Dex-PPy NAs	1422:1436	the Dex-PPy NAs	1422:1436	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	8	73	theme	important	1581:1589	arg1	step					1591:1594	an important step	1578:1594	an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility	1578:1742	Therefore, this study represents an important step towards the scalable synthesis of polypyrrole-based nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	3	74	theme	one-step	703:710	arg1	synthesis					712:720	the one-step synthesis	699:720	the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media	699:871	Herein, a "polymerization/assembly" two-in-one synthesis is proposed for the first time to achieve the one-step synthesis of a new family of polypyrrole-based nano-assemblies, dextran-polypyrrole nano-assemblies (Dex-PPy NAs), under ambient conditions and in aqueous media.					
32507183	2	75	theme	monomers	458:465	arg1	polymerization					432:445	the polymerization	428:445	the polymerization of pyrrole monomers	428:465	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	0	76	theme	enhanced	99:106	arg1	biocompatibility					108:123	enhanced biocompatibility	99:123	enhanced biocompatibility	99:123	Scalable dextran-polypyrrole nano-assemblies with photothermal/photoacoustic dual capabilities and enhanced biocompatibility.					
32507183	7	77	theme	real-time	1453:1461	arg1	imaging					1477:1483	real-time photoacoustic imaging	1453:1483	real-time photoacoustic imaging	1453:1483	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32507183	2	78	theme	nano-assemblies	321:335	arg1	synthesis					290:298	the synthesis	286:298	the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers	286:388	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	2	79	theme	pyrrole	450:456	arg1	monomers					458:465	pyrrole monomers	450:465	pyrrole monomers	450:465	However, the synthesis of polypyrrole-based nano-assemblies which have colloidal stability in biological buffers requires a number of steps, including the polymerization of pyrrole monomers, self-assembly of polypyrrole-based copolymers, and even an additional step to increase the biocompatibility of the nano-assemblies.					
32507183	7	80	theme	in	1385:1386	arg1	study					1393:1397	An in vivo study	1382:1397	An in vivo study	1382:1397	An in vivo study further confirmed that the Dex-PPy NAs have excellent real-time photoacoustic imaging and photothermal therapy capabilities for malignant tumors.					
32330502	0	0	theme	methotrexate	86:97	arg1	composites					99:108	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	composites	99:108	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	6	1	theme	fibrotic	948:955	arg1	tests					924:928	renal function tests	909:928	renal function tests	909:928	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	1	theme	fibrotic	948:955	arg1	markers					957:963	inflammatory and fibrotic markers	931:963	inflammatory and fibrotic markers of renal tissues	931:980	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	5	2	theme	protective	666:675	arg1	role					677:680	their possible protective role	651:680	their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model	651:749	Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model.					
32330502	7	3	theme	nano-fibrillated	1066:1081	arg1	carrier					1093:1099	nano-fibrillated cellulose carrier	1066:1099	nano-fibrillated cellulose carrier	1066:1099	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	7	4	from	carrier	1188:1194	arg1	applications					1215:1226	further medical applications	1199:1226	further medical applications with minimal side effects on kidney tissue in leukemia model	1199:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	4	5	theme	silicon	588:594	arg1	dioxide					596:602	nano silicon dioxide	583:602	nano silicon dioxide	583:602	Methotrexate was chosen to be loaded on nano-fibrillated cellulose and nano silicon dioxide.					
32330502	0	6	theme	dioxide	78:84	arg1	composites					99:108	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	composites	99:108	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	6	7	theme	silicon	855:861	arg1	dioxide					863:869	nano silicon dioxide	850:869	nano silicon dioxide	850:869	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	2	8	theme	rice	359:362	arg1	straw					364:368	rice straw	359:368	rice straw	359:368	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	4	9	theme	nano	583:586	arg1	dioxide					596:602	nano silicon dioxide	583:602	nano silicon dioxide	583:602	Methotrexate was chosen to be loaded on nano-fibrillated cellulose and nano silicon dioxide.					
32330502	7	10	theme	minimal	1233:1239	arg1	effects					1246:1252	minimal side effects	1233:1252	minimal side effects on kidney tissue in leukemia model	1233:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	6	11	theme	inflammatory	931:942	arg1	tests					924:928	renal function tests	909:928	renal function tests	909:928	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	11	theme	inflammatory	931:942	arg1	markers					957:963	inflammatory and fibrotic markers	931:963	inflammatory and fibrotic markers of renal tissues	931:980	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	12	theme	sustained	997:1005	arg1	release					1007:1013	the sustained release	993:1013	the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier	993:1099	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	5	13	theme	methotrexate	610:621	arg1	carriers					623:630	Both methotrexate carriers	605:630	Both methotrexate carriers	605:630	Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model.					
32330502	0	14	theme	composites	99:108	arg1	Application					0:10	Application	0:10	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.	0:154	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	1	15	contain	have	178:181	arg2	got					183:185	got	183:185	got growing interest due to their relative abundance and ability to sustain the release of medicaments	183:284	Cellulose derivatives have got growing interest due to their relative abundance and ability to sustain the release of medicaments.					
32330502	1	15	contain	have	178:181	arg1	derivatives					166:176	Cellulose derivatives	156:176	Cellulose derivatives	156:176	Cellulose derivatives have got growing interest due to their relative abundance and ability to sustain the release of medicaments.					
32330502	5	16	theme	renal	690:694	arg1	fibrosis					696:703	renal fibrosis	690:703	renal fibrosis induced by methotrexate in leukemia rat model	690:749	Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model.					
32330502	7	17	theme	leukemia	1274:1281	arg1	model					1283:1287	leukemia model	1274:1287	leukemia model	1274:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	1	18	theme	medicaments	274:284	arg1	release					263:269	the release	259:269	the release of medicaments	259:284	Cellulose derivatives have got growing interest due to their relative abundance and ability to sustain the release of medicaments.					
32330502	7	19	theme	silicon	1134:1140	arg1	dioxide					1142:1148	nano silicon dioxide	1129:1148	nano silicon dioxide	1129:1148	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	2	20	used	used	374:377	arg2	cellulose					330:338	micro- and nano-fibrillated cellulose	302:338	micro- and nano-fibrillated cellulose	302:338	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	2	20	used	used	374:377	arg2	carriers					387:394	drug carriers	382:394	drug carriers	382:394	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	0	21	theme	renal	118:122	arg1	fibrosis					124:131	renal fibrosis	118:131	renal fibrosis in leukemia rat model	118:153	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	6	22	theme	function	915:922	arg1	tests					924:928	renal function tests	909:928	renal function tests	909:928	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	22	theme	function	915:922	arg1	markers					957:963	inflammatory and fibrotic markers	931:963	inflammatory and fibrotic markers of renal tissues	931:980	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	23	theme	novel	1177:1181	arg1	carrier					1188:1194	an effective novel drug carrier	1164:1194	an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model	1164:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	6	24	theme	nano-fibrillated	820:835	arg1	cellulose					837:845	nano-fibrillated cellulose	820:845	nano-fibrillated cellulose	820:845	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	25	theme	further	1199:1205	arg1	applications					1215:1226	further medical applications	1199:1226	further medical applications with minimal side effects on kidney tissue in leukemia model	1199:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	6	26	theme	renal	909:913	arg1	tests					924:928	renal function tests	909:928	renal function tests	909:928	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	26	theme	renal	909:913	arg1	markers					957:963	inflammatory and fibrotic markers	931:963	inflammatory and fibrotic markers of renal tissues	931:980	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	27	from	tissue	1264:1269	arg1	model					1283:1287	leukemia model	1274:1287	leukemia model	1274:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	7	28	theme	kidney	1257:1262	arg1	tissue					1264:1269	kidney tissue	1257:1269	kidney tissue in leukemia model	1257:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	2	29	theme	nano-fibrillated	313:328	arg1	carriers					387:394	drug carriers	382:394	drug carriers	382:394	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	2	29	theme	nano-fibrillated	313:328	arg1	cellulose					330:338	micro- and nano-fibrillated cellulose	302:338	micro- and nano-fibrillated cellulose	302:338	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	2	30	theme	drug	382:385	arg1	carriers					387:394	drug carriers	382:394	drug carriers	382:394	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	2	30	theme	drug	382:385	arg1	cellulose					330:338	micro- and nano-fibrillated cellulose	302:338	micro- and nano-fibrillated cellulose	302:338	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	7	31	theme	medical	1207:1213	arg1	applications					1215:1226	further medical applications	1199:1226	further medical applications with minimal side effects on kidney tissue in leukemia model	1199:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	4	32	theme	nano-fibrillated	552:567	arg1	cellulose					569:577	nano-fibrillated cellulose	552:577	nano-fibrillated cellulose	552:577	Methotrexate was chosen to be loaded on nano-fibrillated cellulose and nano silicon dioxide.					
32330502	5	33	theme	possible	657:664	arg1	role					677:680	their possible protective role	651:680	their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model	651:749	Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model.					
32330502	7	34	from	effects	1246:1252	arg1	tissue					1264:1269	kidney tissue	1257:1269	kidney tissue in leukemia model	1257:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	0	35	theme	nano	22:25	arg1	methotrexate					49:60	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	methotrexate	49:60	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	0	36	theme	leukemia	136:143	arg1	model					149:153	leukemia rat model	136:153	leukemia rat model	136:153	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	2	37	theme	micro-	302:307	arg1	carriers					387:394	drug carriers	382:394	drug carriers	382:394	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	2	37	theme	micro-	302:307	arg1	cellulose					330:338	micro- and nano-fibrillated cellulose	302:338	micro- and nano-fibrillated cellulose	302:338	In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers.					
32330502	3	38	theme	silicon	452:458	arg1	dioxide					460:466	nano silicon dioxide	447:466	nano silicon dioxide	447:466	Both carriers in addition to another one which is nano silicon dioxide were characterized with various techniques.					
32330502	5	39	theme	leukemia	732:739	arg1	model					745:749	leukemia rat model	732:749	leukemia rat model	732:749	Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model.					
32330502	6	40	contain	have	880:883	arg1	methotrexate					797:808	loading methotrexate	789:808	loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide	789:869	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	40	contain	have	880:883	arg2	role					901:904	an ameliorative role	885:904	an ameliorative role	885:904	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	41	theme	drug	1183:1186	arg1	carrier					1188:1194	an effective novel drug carrier	1164:1194	an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model	1164:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	6	42	from	methotrexate	797:808	arg1	dioxide					863:869	nano silicon dioxide	850:869	nano silicon dioxide	850:869	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	42	from	methotrexate	797:808	arg1	cellulose					837:845	nano-fibrillated cellulose	820:845	nano-fibrillated cellulose	820:845	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	0	43	theme	cellulose	39:47	arg1	methotrexate					49:60	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	methotrexate	49:60	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	5	44	theme	rat	741:743	arg1	model					745:749	leukemia rat model	732:749	leukemia rat model	732:749	Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model.					
32330502	7	45	with	applications	1215:1226	arg1	effects					1246:1252	minimal side effects	1233:1252	minimal side effects on kidney tissue in leukemia model	1233:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	1	46	theme	relative	217:224	arg1	abundance					226:234	their relative abundance	211:234	their relative abundance	211:234	Cellulose derivatives have got growing interest due to their relative abundance and ability to sustain the release of medicaments.					
32330502	0	47	theme	fibrillated	27:37	arg1	methotrexate					49:60	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	methotrexate	49:60	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	0	48	theme	rat	145:147	arg1	model					149:153	leukemia rat model	136:153	leukemia rat model	136:153	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	6	49	theme	ameliorative	888:899	arg1	role					901:904	an ameliorative role	885:904	an ameliorative role	885:904	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	50	theme	loading	789:795	arg1	methotrexate					797:808	loading methotrexate	789:808	loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide	789:869	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	3	51	theme	nano	447:450	arg1	dioxide					460:466	nano silicon dioxide	447:466	nano silicon dioxide	447:466	Both carriers in addition to another one which is nano silicon dioxide were characterized with various techniques.					
32330502	6	52	theme	tissues	974:980	arg1	tests					924:928	renal function tests	909:928	renal function tests	909:928	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	52	theme	tissues	974:980	arg1	markers					957:963	inflammatory and fibrotic markers	931:963	inflammatory and fibrotic markers of renal tissues	931:980	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	53	theme	cellulose	1083:1091	arg1	carrier					1093:1099	nano-fibrillated cellulose carrier	1066:1099	nano-fibrillated cellulose carrier	1066:1099	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	0	54	theme	methotrexate	49:60	arg1	Application					0:10	Application	0:10	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.	0:154	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	6	55	theme	renal	968:972	arg1	tissues					974:980	renal tissues	968:980	renal tissues	968:980	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	0	56	from	fibrosis	124:131	arg1	model					149:153	leukemia rat model	136:153	leukemia rat model	136:153	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	7	57	theme	long	1035:1038	arg1	period					1045:1050	long time period	1035:1050	long time period maintained by nano-fibrillated cellulose carrier	1035:1099	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	3	58	theme	various	492:498	arg1	techniques					500:509	various techniques	492:509	various techniques	492:509	Both carriers in addition to another one which is nano silicon dioxide were characterized with various techniques.					
32330502	7	59	theme	effective	1167:1175	arg1	carrier					1188:1194	an effective novel drug carrier	1164:1194	an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model	1164:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	6	60	theme	nano	850:853	arg1	dioxide					863:869	nano silicon dioxide	850:869	nano silicon dioxide	850:869	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	6	61	theme	study	768:772	arg1	Results					752:758	Results	752:758	Results of this study	752:772	Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues.					
32330502	7	62	theme	methotrexate	1018:1029	arg1	release					1007:1013	the sustained release	993:1013	the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier	993:1099	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	7	63	theme	nano	1129:1132	arg1	dioxide					1142:1148	nano silicon dioxide	1129:1148	nano silicon dioxide	1129:1148	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	0	64	theme	silicon	70:76	arg1	composites					99:108	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	composites	99:108	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	7	65	theme	time	1040:1043	arg1	period					1045:1050	long time period	1035:1050	long time period maintained by nano-fibrillated cellulose carrier	1035:1099	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
32330502	0	66	theme	nano	65:68	arg1	composites					99:108	either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites	15:108	composites	99:108	Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.					
32330502	1	67	theme	Cellulose	156:164	arg1	derivatives					166:176	Cellulose derivatives	156:176	Cellulose derivatives	156:176	Cellulose derivatives have got growing interest due to their relative abundance and ability to sustain the release of medicaments.					
32330502	7	68	theme	side	1241:1244	arg1	effects					1246:1252	minimal side effects	1233:1252	minimal side effects on kidney tissue in leukemia model	1233:1287	Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.					
33151037	7	0	theme	antibiofilm	1101:1111	arg1	properties					1113:1122	excellent antibiofilm properties	1091:1122	excellent antibiofilm properties	1091:1122	What is more, the synergistic effect of CS and Zn2+ make the CS-Zn(II) MNs obtain excellent antibiofilm properties.					
33151037	11	1	theme	promising	1752:1760	arg1	MNs					1744:1746	the CS-Zn(II) MNs	1730:1746	the CS-Zn(II) MNs	1730:1746	These results indicate that the CS-Zn(II) MNs are promising method for bacterial biofilm eradication.					
33151037	11	1	theme	promising	1752:1760	arg1	method					1762:1767	promising method	1752:1767	promising method for bacterial biofilm eradication	1752:1801	These results indicate that the CS-Zn(II) MNs are promising method for bacterial biofilm eradication.					
33151037	9	2	theme	treatment	1532:1540	arg1	time					1542:1545	the treatment time	1528:1545	the treatment time	1528:1545	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	3	3	theme	nitrate	472:478	arg1	MNs					446:448	MNs	446:448	MNs	446:448	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	3	3	theme	nitrate	472:478	arg1	microneedles					432:443	composite microneedles	422:443	composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs)	422:494	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	10	4	contain	possess	1665:1671	arg1	MNs					1661:1663	the CS-Zn(II) MNs	1647:1663	the CS-Zn(II) MNs	1647:1663	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	10	4	contain	possess	1665:1671	arg2	cytocompatibility					1683:1699	brilliant cytocompatibility	1673:1699	brilliant cytocompatibility	1673:1699	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	9	5	theme	antibiofilm	1443:1453	arg1	effect					1455:1460	the antibiofilm effect	1439:1460	the antibiofilm effect of MNs	1439:1467	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	5	6	theme	needle-like	698:708	arg1	structure					710:718	the needle-like structure	694:718	the needle-like structure	694:718	The MNs can pierce the EPS due to the needle-like structure, and can transport directly the CS and Zn2+ into the bacterial biofilm.					
33151037	5	7	dep	CS	752:753	arg1	biofilm					783:789	the bacterial biofilm	769:789	the bacterial biofilm	769:789	The MNs can pierce the EPS due to the needle-like structure, and can transport directly the CS and Zn2+ into the bacterial biofilm.					
33151037	4	8	theme	CS	646:647	arg1	properties					632:641	the antibacterial properties	614:641	the antibacterial properties of CS and Zn2+	614:656	The CS-Zn(II) MNs combined the structure characteristic of MNs with the antibacterial properties of CS and Zn2+ .					
33151037	2	9	theme	polymeric	297:305	arg1	EPS					319:321	EPS	319:321	EPS	319:321	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	2	9	theme	polymeric	297:305	arg1	substances					307:316	extracellular polymeric substances	283:316	extracellular polymeric substances (EPS) produced by the bacteria	283:347	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	8	10	theme	biofilm	1276:1282	arg1	properties					1296:1305	biofilm eradication properties	1276:1305	biofilm eradication properties	1276:1305	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	3	11	theme	bacterial	527:535	arg1	biofilm					537:543	bacterial biofilm	527:543	bacterial biofilm	527:543	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	6	12	theme	MNs	821:823	arg1	structure					808:816	The needle-like structure	792:816	The needle-like structure of MNs	792:823	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	7	13	theme	synergistic	1027:1037	arg1	effect					1039:1044	the synergistic effect	1023:1044	the synergistic effect of CS and Zn2+	1023:1059	What is more, the synergistic effect of CS and Zn2+ make the CS-Zn(II) MNs obtain excellent antibiofilm properties.					
33151037	2	14	theme	extracellular	283:295	arg1	EPS					319:321	EPS	319:321	EPS	319:321	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	2	14	theme	extracellular	283:295	arg1	substances					307:316	extracellular polymeric substances	283:316	extracellular polymeric substances (EPS) produced by the bacteria	283:347	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	4	15	theme	CS-Zn	550:554	arg1	MNs					560:562	The CS-Zn(II) MNs	546:562	The CS-Zn(II) MNs	546:562	The CS-Zn(II) MNs combined the structure characteristic of MNs with the antibacterial properties of CS and Zn2+ .					
33151037	9	16	theme	time	1542:1545	arg1	increase					1516:1523	the increase	1512:1523	the increase of the treatment time	1512:1545	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	9	17	theme	inhibition	1348:1357	arg1	superior					1414:1421	superior	1414:1421	superior	1414:1421	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	9	17	theme	inhibition	1348:1357	arg1	test					1364:1367	The inhibition zone test	1344:1367	The inhibition zone test shown that the antibiofilm effect of MNs	1344:1408	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	8	18	theme	bacterial	1163:1171	arg1	staining					1183:1190	bacterial live/dead staining	1163:1190	bacterial live/dead staining tests	1163:1196	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	5	19	theme	bacterial	773:781	arg1	biofilm					783:789	the bacterial biofilm	769:789	the bacterial biofilm	769:789	The MNs can pierce the EPS due to the needle-like structure, and can transport directly the CS and Zn2+ into the bacterial biofilm.					
33151037	7	20	theme	Zn2+	1056:1059	arg1	effect					1039:1044	the synergistic effect	1023:1044	the synergistic effect of CS and Zn2+	1023:1059	What is more, the synergistic effect of CS and Zn2+ make the CS-Zn(II) MNs obtain excellent antibiofilm properties.					
33151037	6	21	theme	needle-like	946:956	arg1	structure					958:966	needle-like structure	946:966	needle-like structure	946:966	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	1	22	theme	major	178:182	arg1	cause					184:188	a major cause	176:188	a major cause of chronic wounds	176:206	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	22	theme	major	178:182	arg1	biofilm					165:171	bacterial biofilm	155:171	bacterial biofilm	155:171	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	9	23	theme	MNs	1406:1408	arg1	effect					1396:1401	the antibiofilm effect	1380:1401	the antibiofilm effect of MNs	1380:1408	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	6	24	theme	needle-like	796:806	arg1	structure					808:816	The needle-like structure	792:816	The needle-like structure of MNs	792:823	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	4	25	theme	MNs	605:607	arg1	characteristic					587:600	the structure characteristic	573:600	the structure characteristic of MNs	573:607	The CS-Zn(II) MNs combined the structure characteristic of MNs with the antibacterial properties of CS and Zn2+ .					
33151037	1	26	theme	health	97:102	arg1	owing					116:120	health threatening owing	97:120	health threatening owing to the increasing morbidity	97:148	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	26	theme	health	97:102	arg1	wounds					78:83	Chronic wounds	70:83	Chronic wounds	70:83	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	4	27	theme	antibacterial	618:630	arg1	properties					632:641	the antibacterial properties	614:641	the antibacterial properties of CS and Zn2+	614:656	The CS-Zn(II) MNs combined the structure characteristic of MNs with the antibacterial properties of CS and Zn2+ .					
33151037	0	28	theme	Chitosan/zinc	0:12	arg1	nitrate					14:20	Chitosan/zinc nitrate	0:20	Chitosan/zinc nitrate	0:20	Chitosan/zinc nitrate microneedles for bacterial biofilm eradication.					
33151037	8	29	theme	colony	1138:1143	arg1	units					1153:1157	the colony forming units	1134:1157	the colony forming units	1134:1157	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	1	30	theme	threatening	104:114	arg1	owing					116:120	health threatening owing	97:120	health threatening owing to the increasing morbidity	97:148	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	30	theme	threatening	104:114	arg1	wounds					78:83	Chronic wounds	70:83	Chronic wounds	70:83	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	8	31	theme	forming	1145:1151	arg1	units					1153:1157	the colony forming units	1134:1157	the colony forming units	1134:1157	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	11	32	theme	biofilm	1783:1789	arg1	eradication					1791:1801	bacterial biofilm eradication	1773:1801	bacterial biofilm eradication	1773:1801	These results indicate that the CS-Zn(II) MNs are promising method for bacterial biofilm eradication.					
33151037	6	33	theme	drugs	1002:1006	arg1	diffusion					989:997	the diffusion	985:997	the diffusion of drugs	985:1006	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	10	34	theme	bromide	1614:1620	arg1	assays					1628:1633	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays	1557:1633	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays	1557:1633	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	8	35	theme	eradication	1284:1294	arg1	properties					1296:1305	biofilm eradication properties	1276:1305	biofilm eradication properties	1276:1305	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	1	36	theme	chronic	193:199	arg1	wounds					201:206	chronic wounds	193:206	chronic wounds	193:206	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	7	37	theme	CS-Zn	1070:1074	arg1	MNs					1080:1082	the CS-Zn(II) MNs	1066:1082	the CS-Zn(II) MNs	1066:1082	What is more, the synergistic effect of CS and Zn2+ make the CS-Zn(II) MNs obtain excellent antibiofilm properties.					
33151037	6	38	theme	bacterial	882:890	arg1	biofilm					892:898	bacterial biofilm	882:898	bacterial biofilm	882:898	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	2	39	theme	substances	307:316	arg1	barrier					272:278	the barrier	268:278	the barrier of extracellular polymeric substances (EPS) produced by the bacteria	268:347	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	1	40	theme	wounds	201:206	arg1	cause					184:188	a major cause	176:188	a major cause of chronic wounds	176:206	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	40	theme	wounds	201:206	arg1	biofilm					165:171	bacterial biofilm	155:171	bacterial biofilm	155:171	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	40	theme	wounds	201:206	arg1	owing					116:120	health threatening owing	97:120	health threatening owing to the increasing morbidity	97:148	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	40	theme	wounds	201:206	arg1	wounds					78:83	Chronic wounds	70:83	Chronic wounds	70:83	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	0	41	theme	biofilm	49:55	arg1	eradication					57:67	bacterial biofilm eradication	39:67	bacterial biofilm eradication	39:67	Chitosan/zinc nitrate microneedles for bacterial biofilm eradication.					
33151037	1	42	theme	increasing	129:138	arg1	morbidity					140:148	the increasing morbidity	125:148	the increasing morbidity	125:148	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	8	43	dep	100	1255:1257	arg1	to					1252:1253	to	1252:1253	to	1252:1253	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	9	44	theme	MNs	1465:1467	arg1	effect					1455:1460	the antibiofilm effect	1439:1460	the antibiofilm effect of MNs	1439:1467	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	8	45	theme	staining	1183:1190	arg1	tests					1192:1196	bacterial live/dead staining tests	1163:1196	bacterial live/dead staining tests	1163:1196	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	0	46	theme	bacterial	39:47	arg1	eradication					57:67	bacterial biofilm eradication	39:67	bacterial biofilm eradication	39:67	Chitosan/zinc nitrate microneedles for bacterial biofilm eradication.					
33151037	6	47	dep	carrier	870:876	arg1	%					912:912	nearly 14-23%	900:912	nearly 14-23%	900:912	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	2	48	theme	bacterial	226:234	arg1	biofilm					236:242	bacterial biofilm	226:242	bacterial biofilm	226:242	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	8	49	theme	live/dead	1173:1181	arg1	staining					1183:1190	bacterial live/dead staining	1163:1190	bacterial live/dead staining tests	1163:1196	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	7	50	theme	CS	1049:1050	arg1	effect					1039:1044	the synergistic effect	1023:1044	the synergistic effect of CS and Zn2+	1023:1059	What is more, the synergistic effect of CS and Zn2+ make the CS-Zn(II) MNs obtain excellent antibiofilm properties.					
33151037	6	51	theme	drug	865:868	arg1	carrier					870:876	drug carrier	865:876	drug carrier	865:876	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	8	52	theme	MNs	1339:1341	arg1	abilities					1238:1246	the fascinating antibacterial abilities	1208:1246	the fascinating antibacterial abilities (up to 100% inhibition)	1208:1270	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	8	52	theme	MNs	1339:1341	arg1	properties					1296:1305	biofilm eradication properties	1276:1305	biofilm eradication properties	1276:1305	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	8	52	theme	MNs	1339:1341	arg1	inhibition					1260:1269	up to 100% inhibition	1249:1269	up to 100% inhibition	1249:1269	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	9	53	theme	zone	1359:1362	arg1	superior					1414:1421	superior	1414:1421	superior	1414:1421	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	9	53	theme	zone	1359:1362	arg1	test					1364:1367	The inhibition zone test	1344:1367	The inhibition zone test shown that the antibiofilm effect of MNs	1344:1408	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	8	54	theme	antibacterial	1224:1236	arg1	abilities					1238:1246	the fascinating antibacterial abilities	1208:1246	the fascinating antibacterial abilities (up to 100% inhibition)	1208:1270	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	8	54	theme	antibacterial	1224:1236	arg1	inhibition					1260:1269	up to 100% inhibition	1249:1269	up to 100% inhibition	1249:1269	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	9	55	theme	antibiofilm	1384:1394	arg1	effect					1396:1401	the antibiofilm effect	1380:1401	the antibiofilm effect of MNs	1380:1408	The inhibition zone test shown that the antibiofilm effect of MNs was superior to membrane and the antibiofilm effect of MNs was become increasingly obvious along with the increase of the treatment time.					
33151037	2	56	theme	critical	213:220	arg1	eradication					244:254	The critical for bacterial biofilm eradication	209:254	The critical for bacterial biofilm eradication	209:254	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	7	57	theme	excellent	1091:1099	arg1	properties					1113:1122	excellent antibiofilm properties	1091:1122	excellent antibiofilm properties	1091:1122	What is more, the synergistic effect of CS and Zn2+ make the CS-Zn(II) MNs obtain excellent antibiofilm properties.					
33151037	3	58	theme	zinc	467:470	arg1	MNs					491:493	CS-Zn[II] MNs	481:493	CS-Zn[II] MNs	481:493	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	3	58	theme	zinc	467:470	arg1	nitrate					472:478	zinc nitrate	467:478	zinc nitrate (CS-Zn[II] MNs)	467:494	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	10	59	theme	brilliant	1673:1681	arg1	cytocompatibility					1683:1699	brilliant cytocompatibility	1673:1699	brilliant cytocompatibility	1673:1699	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	11	60	theme	CS-Zn	1734:1738	arg1	MNs					1744:1746	the CS-Zn(II) MNs	1730:1746	the CS-Zn(II) MNs	1730:1746	These results indicate that the CS-Zn(II) MNs are promising method for bacterial biofilm eradication.					
33151037	11	60	theme	CS-Zn	1734:1738	arg1	method					1762:1767	promising method	1752:1767	promising method for bacterial biofilm eradication	1752:1801	These results indicate that the CS-Zn(II) MNs are promising method for bacterial biofilm eradication.					
33151037	10	61	theme	CS-Zn	1651:1655	arg1	MNs					1661:1663	the CS-Zn(II) MNs	1647:1663	the CS-Zn(II) MNs	1647:1663	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	8	62	theme	fascinating	1212:1222	arg1	abilities					1238:1246	the fascinating antibacterial abilities	1208:1246	the fascinating antibacterial abilities (up to 100% inhibition)	1208:1270	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	8	62	theme	fascinating	1212:1222	arg1	inhibition					1260:1269	up to 100% inhibition	1249:1269	up to 100% inhibition	1249:1269	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	8	63	theme	%	1258:1258	arg1	abilities					1238:1246	the fascinating antibacterial abilities	1208:1246	the fascinating antibacterial abilities (up to 100% inhibition)	1208:1270	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	8	63	theme	%	1258:1258	arg1	inhibition					1260:1269	up to 100% inhibition	1249:1269	up to 100% inhibition	1249:1269	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	1	64	theme	Chronic	70:76	arg1	owing					116:120	health threatening owing	97:120	health threatening owing to the increasing morbidity	97:148	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	64	theme	Chronic	70:76	arg1	wounds					78:83	Chronic wounds	70:83	Chronic wounds	70:83	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	3	65	theme	chitosan	454:461	arg1	MNs					446:448	MNs	446:448	MNs	446:448	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	3	65	theme	chitosan	454:461	arg1	microneedles					432:443	composite microneedles	422:443	composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs)	422:494	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	3	66	theme	composite	422:430	arg1	MNs					446:448	MNs	446:448	MNs	446:448	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	3	66	theme	composite	422:430	arg1	microneedles					432:443	composite microneedles	422:443	composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs)	422:494	In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm.					
33151037	5	67	theme	due	687:689	arg1	EPS					683:685	the EPS	679:685	the EPS due to the needle-like structure	679:718	The MNs can pierce the EPS due to the needle-like structure, and can transport directly the CS and Zn2+ into the bacterial biofilm.					
33151037	11	68	theme	bacterial	1773:1781	arg1	eradication					1791:1801	bacterial biofilm eradication	1773:1801	bacterial biofilm eradication	1773:1801	These results indicate that the CS-Zn(II) MNs are promising method for bacterial biofilm eradication.					
33151037	4	69	theme	structure	577:585	arg1	characteristic					587:600	the structure characteristic	573:600	the structure characteristic of MNs	573:607	The CS-Zn(II) MNs combined the structure characteristic of MNs with the antibacterial properties of CS and Zn2+ .					
33151037	10	70	theme	-2,5-diphenyltetrazolium	1589:1612	arg1	MTT					1623:1625	MTT	1623:1625	MTT	1623:1625	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	10	70	theme	-2,5-diphenyltetrazolium	1589:1612	arg1	bromide					1614:1620	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	1557:1620	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays	1557:1633	Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility.					
33151037	2	71	theme	drugs	379:383	arg1	diffusion					366:374	the diffusion	362:374	the diffusion of drugs within the biofilm	362:402	The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm.					
33151037	6	72	theme	contact	844:850	arg1	area					852:855	the contact area	840:855	the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane	840:936	The needle-like structure of MNs also increased the contact area between drug carrier and bacterial biofilm nearly 14-23% comparing with membrane without needle-like structure, and facilitated the diffusion of drugs.					
33151037	8	73	theme	CS-Zn	1329:1333	arg1	MNs					1339:1341	the CS-Zn(II) MNs	1325:1341	the CS-Zn(II) MNs	1325:1341	Counting the colony forming units and bacterial live/dead staining tests confirmed the fascinating antibacterial abilities (up to 100% inhibition) and biofilm eradication properties, respectively, of the CS-Zn(II) MNs.					
33151037	1	74	theme	bacterial	155:163	arg1	cause					184:188	a major cause	176:188	a major cause of chronic wounds	176:206	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	1	74	theme	bacterial	155:163	arg1	biofilm					165:171	bacterial biofilm	155:171	bacterial biofilm	155:171	Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds.					
33151037	4	75	theme	Zn2+	653:656	arg1	properties					632:641	the antibacterial properties	614:641	the antibacterial properties of CS and Zn2+	614:656	The CS-Zn(II) MNs combined the structure characteristic of MNs with the antibacterial properties of CS and Zn2+ .					
34804990	0	0	from	Inflammation	41:52	arg1	Rats					92:95	Rats	92:95	Rats	92:95	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	3	1	theme	good	516:519	arg1	effect					533:538	a good therapeutic effect	514:538	a good therapeutic effect	514:538	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	7	2	theme	flora	1378:1382	arg1	pathway					1433:1439	an important pathway	1420:1439	an important pathway	1420:1439	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	7	2	theme	flora	1378:1382	arg1	regulation					1353:1362	the regulation	1349:1362	the regulation of intestinal flora by Pulsatilla chinensis saponins	1349:1415	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	5	3	theme	mesenteric	1044:1053	arg1	characteristics					1071:1085	the mesenteric hemorheological characteristics	1040:1085	the mesenteric hemorheological characteristics	1040:1085	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	3	4	theme	therapeutic	521:531	arg1	effect					533:538	a good therapeutic effect	514:538	a good therapeutic effect	514:538	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	5	5	theme	hemorheological	1055:1069	arg1	characteristics					1071:1085	the mesenteric hemorheological characteristics	1040:1085	the mesenteric hemorheological characteristics	1040:1085	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	6	6	theme	PRS	1300:1302	arg1	effects					1289:1295	the anti-UC effects	1277:1295	the anti-UC effects of PRS	1277:1302	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	7	7	theme	anti-UC	1449:1455	arg1	activity					1457:1464	its anti-UC activity	1445:1464	its anti-UC activity	1445:1464	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	4	8	theme	PRS	820:822	arg1	mechanism					807:815	the mechanism	803:815	the mechanism of PRS	803:822	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	6	9	theme	inflammatory	1208:1219	arg1	response					1221:1228	the inflammatory response	1204:1228	the inflammatory response	1204:1228	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	0	10	dep	Composition	115:125	arg1	the					111:113	the	111:113	the	111:113	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	3	11	theme	ulcerative	543:552	arg1	UC					563:564	UC	563:564	UC	563:564	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	3	11	theme	ulcerative	543:552	arg1	colitis					554:560	ulcerative colitis	543:560	ulcerative colitis (UC)	543:565	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	5	12	theme	fecal	852:856	arg1	samples					868:874	The fecal and colon samples	848:874	The fecal and colon samples	848:874	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	7	13	theme	intestinal	1367:1376	arg1	flora					1378:1382	intestinal flora	1367:1382	intestinal flora	1367:1382	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	0	14	theme	chinensis	11:19	arg1	Saponins					21:28	Pulsatilla chinensis Saponins	0:28	Pulsatilla chinensis Saponins	0:28	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	3	15	contain	has	510:512	arg2	effect					533:538	a good therapeutic effect	514:538	a good therapeutic effect	514:538	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	3	15	contain	has	510:512	arg1	decoction					500:508	Pulsatilla decoction	489:508	Pulsatilla decoction	489:508	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	5	16	theme	colon	862:866	arg1	samples					868:874	The fecal and colon samples	848:874	The fecal and colon samples	848:874	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	5	17	from	changes	916:922	arg1	diversity					947:955	diversity	947:955	diversity	947:955	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	5	17	from	changes	916:922	arg1	composition					931:941	composition	931:941	composition	931:941	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	5	18	dep	composition	931:941	arg1	the					927:929	the	927:929	the	927:929	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	0	19	theme	Pulsatilla	0:9	arg1	Saponins					21:28	Pulsatilla chinensis Saponins	0:28	Pulsatilla chinensis Saponins	0:28	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	1	20	theme	cooling	276:282	arg1	blood					284:288	cooling blood	276:288	cooling blood	276:288	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	4	21	theme	anti-UC	705:711	arg1	activity					713:720	the possible anti-UC activity	692:720	the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model	692:782	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	2	22	theme	pharmacological	437:451	arg1	activities					453:462	these pharmacological activities	431:462	these pharmacological activities	431:462	Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities.					
34804990	6	23	theme	anti-UC	1281:1287	arg1	effects					1289:1295	the anti-UC effects	1277:1295	the anti-UC effects of PRS	1277:1302	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	4	24	theme	PRS	725:727	arg1	activity					713:720	the possible anti-UC activity	692:720	the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model	692:782	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	7	25	theme	same	1312:1315	arg1	time					1317:1320	the same time	1308:1320	the same time	1308:1320	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	7	26	from	decrease	1603:1610	arg1	bacteria					1519:1526	beneficial bacteria	1508:1526	beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014	1508:1596	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	7	26	from	decrease	1603:1610	arg1	Bacteroides					1627:1637	the harmful Bacteroides	1615:1637	the harmful Bacteroides	1615:1637	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	4	27	theme	study	667:671	arg1	purpose					651:657	the purpose	647:657	the purpose of this study	647:671	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	3	28	from	effect	593:598	arg1	UC					610:611	UC	610:611	UC	610:611	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	1	29	theme	stopping	291:298	arg1	dysentery					300:308	stopping dysentery	291:308	stopping dysentery	291:308	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	0	30	theme	Flora	155:159	arg1	Diversity					131:139	Diversity	131:139	Diversity	131:139	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	0	30	theme	Flora	155:159	arg1	Composition					115:125	Composition	115:125	Composition	115:125	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	5	31	theme	pathological	986:997	arg1	sections					1005:1012	pathological colon sections	986:1012	pathological colon sections	986:1012	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	4	32	theme	rat	774:776	arg1	model					778:782	a dextran sulfate sodium (DSS)-induced rat model	735:782	a dextran sulfate sodium (DSS)-induced rat model	735:782	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	1	33	theme	used	211:214	arg1	Regel					191:195	Regel	191:195	Regel	191:195	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	1	33	theme	used	211:214	arg1	medicine					224:231	a commonly used Chinese medicine	200:231	a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects	200:339	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	7	34	theme	Pulsatilla	1387:1396	arg1	saponins					1408:1415	Pulsatilla chinensis saponins	1387:1415	Pulsatilla chinensis saponins	1387:1415	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	0	35	theme	Intestinal	144:153	arg1	Flora					155:159	Intestinal Flora	144:159	Intestinal Flora	144:159	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	5	36	theme	colon	999:1003	arg1	sections					1005:1012	pathological colon sections	986:1012	pathological colon sections	986:1012	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	4	37	theme	-induced	765:772	arg1	model					778:782	a dextran sulfate sodium (DSS)-induced rat model	735:782	a dextran sulfate sodium (DSS)-induced rat model	735:782	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	1	38	theme	Chinese	216:222	arg1	Regel					191:195	Regel	191:195	Regel	191:195	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	1	38	theme	Chinese	216:222	arg1	medicine					224:231	a commonly used Chinese medicine	200:231	a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects	200:339	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	0	39	theme	Ulcerative	70:79	arg1	Colitis					81:87	DSS-Induced Ulcerative Colitis	58:87	DSS-Induced Ulcerative Colitis	58:87	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	0	40	from	Colitis	81:87	arg1	Rats					92:95	Rats	92:95	Rats	92:95	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	7	41	from	increase	1496:1503	arg1	bacteria					1519:1526	beneficial bacteria	1508:1526	beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014	1508:1596	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	7	41	from	increase	1496:1503	arg1	Bacteroides					1627:1637	the harmful Bacteroides	1615:1637	the harmful Bacteroides	1615:1637	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	2	42	theme	chinensis	353:361	arg1	PRS					373:375	PRS	373:375	PRS	373:375	Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities.					
34804990	2	42	theme	chinensis	353:361	arg1	saponins					363:370	Pulsatilla chinensis saponins	342:370	Pulsatilla chinensis saponins (PRS)	342:376	Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities.					
34804990	0	43	theme	DSS-Induced	58:68	arg1	Colitis					81:87	DSS-Induced Ulcerative Colitis	58:87	DSS-Induced Ulcerative Colitis	58:87	Pulsatilla chinensis Saponins Ameliorate Inflammation and DSS-Induced Ulcerative Colitis in Rats by Regulating the Composition and Diversity of Intestinal Flora.					
34804990	7	44	theme	beneficial	1508:1517	arg1	bacteria					1519:1526	beneficial bacteria	1508:1526	beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014	1508:1596	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	3	45	theme	therapeutic	581:591	arg1	effect					593:598	the therapeutic effect	577:598	the therapeutic effect of PRS on UC	577:611	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	2	46	theme	Pulsatilla	342:351	arg1	PRS					373:375	PRS	373:375	PRS	373:375	Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities.					
34804990	2	46	theme	Pulsatilla	342:351	arg1	saponins					363:370	Pulsatilla chinensis saponins	342:370	Pulsatilla chinensis saponins (PRS)	342:376	Pulsatilla chinensis saponins (PRS) have been identified to be responsible for producing these pharmacological activities.					
34804990	7	47	theme	chinensis	1398:1406	arg1	saponins					1408:1415	Pulsatilla chinensis saponins	1387:1415	Pulsatilla chinensis saponins	1387:1415	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	1	48	theme	anti-inflammatory	315:331	arg1	effects					333:339	anti-inflammatory effects	315:339	anti-inflammatory effects	315:339	Pulsatilla chinensis (Bunge) Regel is a commonly used Chinese medicine for clearing away heat and detoxification, cooling blood, stopping dysentery, and anti-inflammatory effects.					
34804990	4	49	theme	UC	844:845	arg1	treatment					831:839	the treatment	827:839	the treatment of UC	827:845	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	5	50	theme	intestinal	964:973	arg1	flora					975:979	the intestinal flora	960:979	the intestinal flora	960:979	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	6	51	theme	blood	1153:1157	arg1	flow					1159:1162	the mesenteric blood flow	1138:1162	the mesenteric blood flow in UC rats	1138:1173	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	6	52	theme	UC	1167:1168	arg1	rats					1170:1173	UC rats	1167:1173	UC rats	1167:1173	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	3	53	theme	Pulsatilla	489:498	arg1	decoction					500:508	Pulsatilla decoction	489:508	Pulsatilla decoction	489:508	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
34804990	6	54	from	flow	1159:1162	arg1	rats					1170:1173	UC rats	1167:1173	UC rats	1167:1173	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	5	55	theme	flora	975:979	arg1	diversity					947:955	diversity	947:955	diversity	947:955	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	5	55	theme	flora	975:979	arg1	composition					931:941	composition	931:941	composition	931:941	The fecal and colon samples were collected from rats to monitor the changes in the composition and diversity of the intestinal flora, and pathological colon sections were also made to examine the mesenteric hemorheological characteristics.					
34804990	6	56	theme	mesenteric	1142:1151	arg1	flow					1159:1162	the mesenteric blood flow	1138:1162	the mesenteric blood flow in UC rats	1138:1173	The results showed that PRS significantly reduced the mesenteric blood flow in UC rats and significantly alleviated the inflammatory response, which indicates that saponins are involved in the anti-UC effects of PRS.					
34804990	7	57	theme	harmful	1619:1625	arg1	Bacteroides					1627:1637	the harmful Bacteroides	1615:1637	the harmful Bacteroides	1615:1637	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	4	58	theme	possible	696:703	arg1	activity					713:720	the possible anti-UC activity	692:720	the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model	692:782	Therefore, the purpose of this study was to investigate the possible anti-UC activity of PRS using a dextran sulfate sodium (DSS)-induced rat model, and further study the mechanism of PRS in the treatment of UC.					
34804990	7	59	theme	important	1423:1431	arg1	pathway					1433:1439	an important pathway	1420:1439	an important pathway	1420:1439	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	7	59	theme	important	1423:1431	arg1	regulation					1353:1362	the regulation	1349:1362	the regulation of intestinal flora by Pulsatilla chinensis saponins	1349:1415	At the same time, it is also suggested that the regulation of intestinal flora by Pulsatilla chinensis saponins is an important pathway for its anti-UC activity, which may be ascribed to the increase in beneficial bacteria like norank_F_Muribaculaceae and norank_F_norank_O_Clostridia_UCG-014, and decrease in the harmful Bacteroides.					
34804990	3	60	theme	PRS	603:605	arg1	effect					593:598	the therapeutic effect	577:598	the therapeutic effect of PRS on UC	577:611	Studies have shown that Pulsatilla decoction has a good therapeutic effect on ulcerative colitis (UC), however, the therapeutic effect of PRS on UC has not been reported.					
32869322	0	0	theme	navy	82:85	arg1	starches					101:108	navy bean and corn starches	82:108	navy bean and corn starches	82:108	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	7	1	theme	starch	1001:1006	arg1	film					1008:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	2	2	theme	seeds	310:314	arg1	component					288:296	the major component	278:296	the major component of dry bean seeds	278:314	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	2	3	theme	edible	340:345	arg1	films					347:351	edible films	340:351	edible films	340:351	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	2	4	theme	bean	305:308	arg1	seeds					310:314	dry bean seeds	301:314	dry bean seeds	301:314	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	7	5	theme	water	1155:1159	arg1	barrier					1167:1173	the water vapor barrier	1151:1173	the water vapor barrier	1151:1173	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	7	6	theme	Navy	991:994	arg1	film					1008:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	4	7	from	g L-1	653:657	arg1	oil					643:645	Sunflower oil	633:645	Sunflower oil at 10 g L-1	633:657	Sunflower oil at 10 g L-1 was dispersed into film-forming solution to design composite films.					
32869322	3	8	dep	chose	404:408	arg1	isolated					448:455	isolated	448:455	isolated its starch component	448:476	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	8	dep	chose	404:408	arg1	compared					553:560	compared	553:560	compared these with the films made using isolated and commercial corn starches	553:630	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	8	dep	chose	404:408	arg1	prepared					479:486	prepared	479:486	prepared edible films with different formulations (35 and 40 g L-1 )	479:546	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	0	9	theme	bean	87:90	arg1	starches					101:108	navy bean and corn starches	82:108	navy bean and corn starches	82:108	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	3	10	theme	corn	618:621	arg1	starches					623:630	commercial corn starches	607:630	commercial corn starches	607:630	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	7	11	theme	bean	996:999	arg1	film					1008:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	3	12	with	films	495:499	arg1	formulations					516:527	different formulations	506:527	different formulations (35 and 40 g L-1 )	506:546	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	7	13	theme	oil	1125:1127	arg1	addition					1103:1110	the addition	1099:1110	the addition of sunflower oil	1099:1127	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	3	14	theme	navy	410:413	arg1	bean					415:418	navy bean	410:418	navy bean	410:418	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	14	theme	navy	410:413	arg1	source					440:445	a model dry bean source	423:445	a model dry bean source	423:445	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	7	15	theme	starch	1081:1086	arg1	films					1088:1092	corn starch films	1076:1092	corn starch films	1076:1092	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	5	16	theme	films	812:816	arg1	microstructure					787:800	microstructure	787:800	microstructure	787:800	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	16	theme	films	812:816	arg1	property					751:758	The water vapor barrier property	727:758	The water vapor barrier property	727:758	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	16	theme	films	812:816	arg1	properties					772:781	mechanical properties	761:781	mechanical properties	761:781	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	7	17	theme	comparable	1062:1071	arg1	properties					1051:1060	physicochemical and mechanical properties	1020:1060	physicochemical and mechanical properties comparable to corn starch films	1020:1092	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	5	18	from	microstructure	787:800	arg1	corn					837:840	corn	837:840	corn	837:840	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	18	from	microstructure	787:800	arg1	bean					828:831	navy bean	823:831	navy bean	823:831	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	6	19	dep	smooth	931:936	arg1	surface					950:956	surface	950:956	surface	950:956	All of the films had smooth and uniform surface and were transparent.					
32869322	7	20	theme	films	1204:1208	arg1	properties					1190:1199	mechanical properties	1179:1199	mechanical properties of films	1179:1208	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	7	20	theme	films	1204:1208	arg1	barrier					1167:1173	the water vapor barrier	1151:1173	the water vapor barrier	1151:1173	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	1	21	theme	great	149:153	arg1	interest					155:162	a great interest	147:162	a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.)	147:237	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	0	22	theme	corn	96:99	arg1	starches					101:108	navy bean and corn starches	82:108	navy bean and corn starches	82:108	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	5	23	theme	starch	805:810	arg1	films					812:816	starch films	805:816	starch films from navy bean and corn	805:840	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	7	24	theme	CONCLUSION	980:989	arg1	film					1008:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film	980:1011	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	1	25	dep	BACKGROUND	111:120	arg1	interest					155:162	a great interest	147:162	a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.)	147:237	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	3	26	theme	commercial	607:616	arg1	starches					623:630	commercial corn starches	607:630	commercial corn starches	607:630	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	5	27	from	bean	828:831	arg1	properties					772:781	mechanical properties	761:781	mechanical properties	761:781	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	27	from	bean	828:831	arg1	films					812:816	starch films	805:816	starch films from navy bean and corn	805:840	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	27	from	bean	828:831	arg1	property					751:758	The water vapor barrier property	727:758	The water vapor barrier property	727:758	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	27	from	bean	828:831	arg1	microstructure					787:800	microstructure	787:800	microstructure	787:800	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	4	28	theme	Sunflower	633:641	arg1	oil					643:645	Sunflower oil	633:645	Sunflower oil at 10 g L-1	633:657	Sunflower oil at 10 g L-1 was dispersed into film-forming solution to design composite films.					
32869322	5	29	from	use	887:889	arg1	packaging					899:907	food packaging	894:907	food packaging	894:907	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	30	theme	mechanical	761:770	arg1	properties					772:781	mechanical properties	761:781	mechanical properties	761:781	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	7	31	theme	corn	1076:1079	arg1	films					1088:1092	corn starch films	1076:1092	corn starch films	1076:1092	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	5	32	from	properties	772:781	arg1	corn					837:840	corn	837:840	corn	837:840	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	32	from	properties	772:781	arg1	bean					828:831	navy bean	823:831	navy bean	823:831	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	3	33	theme	starch	461:466	arg1	component					468:476	its starch component	457:476	its starch component	457:476	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	1	34	theme	new	178:180	arg1	applications					182:193	new applications	178:193	new applications of edible dry beans (Phaseolus vulgaris L.)	178:237	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	0	35	theme	physicochemical	14:28	arg1	properties					45:54	physicochemical and mechanical properties	14:54	physicochemical and mechanical properties of edible films	14:70	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	9	36	theme	Chemical	1349:1356	arg1	Industry					1358:1365	Chemical Industry	1349:1365	Chemical Industry	1349:1365	© 2020 Society of Chemical Industry.					
32869322	8	37	theme	present	1240:1246	arg1	study					1248:1252	the present study	1236:1252	the present study	1236:1252	The findings obtained in the present study demonstrate the potential of using navy bean starch to prepare edible films.					
32869322	2	38	theme	starch	259:264	arg1	utilization					244:254	The utilization	240:254	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films	240:351	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	5	39	theme	water	731:735	arg1	barrier					743:749	The water vapor barrier	727:749	The water vapor barrier property	727:758	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	0	40	theme	properties	45:54	arg1	Comparison					0:9	Comparison	0:9	Comparison of physicochemical and mechanical properties of edible films	0:70	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	7	41	theme	mechanical	1179:1188	arg1	properties					1190:1199	mechanical properties	1179:1199	mechanical properties of films	1179:1208	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	5	42	theme	vapor	737:741	arg1	barrier					743:749	The water vapor barrier	727:749	The water vapor barrier property	727:758	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	4	43	theme	composite	710:718	arg1	films					720:724	design composite films	703:724	design composite films	703:724	Sunflower oil at 10 g L-1 was dispersed into film-forming solution to design composite films.					
32869322	3	44	theme	present	386:392	arg1	study					394:398	the present study	382:398	the present study	382:398	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	0	45	theme	mechanical	34:43	arg1	properties					45:54	physicochemical and mechanical properties	14:54	physicochemical and mechanical properties of edible films	14:70	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	7	46	theme	mechanical	1040:1049	arg1	properties					1051:1060	physicochemical and mechanical properties	1020:1060	physicochemical and mechanical properties comparable to corn starch films	1020:1092	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	3	47	theme	different	506:514	arg1	formulations					516:527	different formulations	506:527	different formulations (35 and 40 g L-1 )	506:546	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	5	48	theme	barrier	743:749	arg1	property					751:758	The water vapor barrier property	727:758	The water vapor barrier property	727:758	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	2	49	theme	films	347:351	arg1	preparation					325:335	the preparation	321:335	the preparation of edible films	321:351	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	0	50	theme	edible	59:64	arg1	films					66:70	edible films	59:70	edible films	59:70	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	1	51	dep	beans	209:213	arg1	vulgaris					226:233	Phaseolus vulgaris	216:233	Phaseolus vulgaris L.	216:236	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	3	52	theme	edible	488:493	arg1	films					495:499	edible films	488:499	edible films with different formulations (35 and 40 g L-1 )	488:546	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	2	53	theme	dry	301:303	arg1	seeds					310:314	dry bean seeds	301:314	dry bean seeds	301:314	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	7	54	theme	physicochemical	1020:1034	arg1	properties					1051:1060	physicochemical and mechanical properties	1020:1060	physicochemical and mechanical properties comparable to corn starch films	1020:1092	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	8	55	theme	bean	1294:1297	arg1	starch					1299:1304	navy bean starch	1289:1304	navy bean starch	1289:1304	The findings obtained in the present study demonstrate the potential of using navy bean starch to prepare edible films.					
32869322	8	56	theme	navy	1289:1292	arg1	starch					1299:1304	navy bean starch	1289:1304	navy bean starch	1289:1304	The findings obtained in the present study demonstrate the potential of using navy bean starch to prepare edible films.					
32869322	3	57	theme	model	425:429	arg1	bean					415:418	navy bean	410:418	navy bean	410:418	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	57	theme	model	425:429	arg1	source					440:445	a model dry bean source	423:445	a model dry bean source	423:445	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	4	58	theme	film-forming	678:689	arg1	solution					691:698	film-forming solution	678:698	film-forming solution to design composite films	678:724	Sunflower oil at 10 g L-1 was dispersed into film-forming solution to design composite films.					
32869322	4	59	theme	design	703:708	arg1	films					720:724	design composite films	703:724	design composite films	703:724	Sunflower oil at 10 g L-1 was dispersed into film-forming solution to design composite films.					
32869322	5	60	theme	navy	823:826	arg1	bean					828:831	navy bean	823:831	navy bean	823:831	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	1	61	theme	dry	205:207	arg1	beans					209:213	edible dry beans	198:213	edible dry beans (Phaseolus vulgaris L.)	198:237	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	7	62	theme	vapor	1161:1165	arg1	barrier					1167:1173	the water vapor barrier	1151:1173	the water vapor barrier	1151:1173	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	1	63	dep	vulgaris	226:233	arg1	L					235:235	L	235:235	Phaseolus vulgaris L.	216:236	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	2	64	theme	major	282:286	arg1	component					288:296	the major component	278:296	the major component of dry bean seeds	278:314	The utilization of starch, comprising the major component of dry bean seeds, for the preparation of edible films has just emerged.					
32869322	1	65	theme	beans	209:213	arg1	applications					182:193	new applications	178:193	new applications of edible dry beans (Phaseolus vulgaris L.)	178:237	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	7	66	theme	sunflower	1115:1123	arg1	oil					1125:1127	sunflower oil	1115:1127	sunflower oil	1115:1127	CONCLUSION Navy bean starch film showed physicochemical and mechanical properties comparable to corn starch films, and the addition of sunflower oil could further improve the water vapor barrier and mechanical properties of films.					
32869322	1	67	theme	edible	198:203	arg1	beans					209:213	edible dry beans	198:213	edible dry beans (Phaseolus vulgaris L.)	198:237	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32869322	8	68	theme	edible	1317:1322	arg1	films					1324:1328	edible films	1317:1328	edible films	1317:1328	The findings obtained in the present study demonstrate the potential of using navy bean starch to prepare edible films.					
32869322	3	69	dep	formulations	516:527	arg1	40 g L-1					537:544	40 g L-1	537:544	40 g L-1	537:544	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	69	dep	formulations	516:527	arg1	35					530:531	35	530:531	35	530:531	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	70	theme	dry	431:433	arg1	bean					415:418	navy bean	410:418	navy bean	410:418	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	70	theme	dry	431:433	arg1	source					440:445	a model dry bean source	423:445	a model dry bean source	423:445	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	5	71	from	corn	837:840	arg1	properties					772:781	mechanical properties	761:781	mechanical properties	761:781	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	71	from	corn	837:840	arg1	films					812:816	starch films	805:816	starch films from navy bean and corn	805:840	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	71	from	corn	837:840	arg1	property					751:758	The water vapor barrier property	727:758	The water vapor barrier property	727:758	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	71	from	corn	837:840	arg1	microstructure					787:800	microstructure	787:800	microstructure	787:800	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	0	72	theme	films	66:70	arg1	properties					45:54	physicochemical and mechanical properties	14:54	physicochemical and mechanical properties of edible films	14:70	Comparison of physicochemical and mechanical properties of edible films made from navy bean and corn starches.					
32869322	5	73	theme	food	894:897	arg1	packaging					899:907	food packaging	894:907	food packaging	894:907	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	74	from	property	751:758	arg1	corn					837:840	corn	837:840	corn	837:840	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	5	74	from	property	751:758	arg1	bean					828:831	navy bean	823:831	navy bean	823:831	The water vapor barrier property, mechanical properties and microstructure of starch films from navy bean and corn were studied to evaluate their potential for use in food packaging.					
32869322	3	75	theme	bean	435:438	arg1	bean					415:418	navy bean	410:418	navy bean	410:418	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	3	75	theme	bean	435:438	arg1	source					440:445	a model dry bean source	423:445	a model dry bean source	423:445	RESULTS In the present study, we chose navy bean as a model dry bean source, isolated its starch component, prepared edible films with different formulations (35 and 40 g L-1 ), and compared these with the films made using isolated and commercial corn starches.					
32869322	1	76	theme	Phaseolus	216:224	arg1	vulgaris					226:233	Phaseolus vulgaris	216:233	Phaseolus vulgaris L.	216:236	BACKGROUND Recently, there has been a great interest in developing new applications of edible dry beans (Phaseolus vulgaris L.).					
32129125	3	0	theme	measured	699:706	arg1	values					722:727	the measured and predicted values	695:727	values	722:727	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	4	1	theme	0.93	843:846	arg1	R2					837:838	an R2	834:838	an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10	834:904	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	5	2	theme	predicted	1008:1016	arg1	values					1018:1023	the measured and predicted values	991:1023	values	1018:1023	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	1	3	theme	study	161:165	arg1	objective					143:151	The objective	139:151	The objective of this study	139:165	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	4	4	theme	size	766:769	arg1	model					771:775	the droplet size model	754:775	the droplet size model	754:775	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	0	5	theme	spraying	75:82	arg1	angle					84:88	spraying angle	75:88	spraying angle	75:88	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	6	6	theme	spraying	1227:1234	arg1	angle					1236:1240	spraying angle	1227:1240	spraying angle	1227:1240	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	6	7	from	size	1218:1221	arg1	process					1249:1255	the process	1245:1255	the process of pharmaceutical fluidized bed granulation	1245:1299	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	1	8	theme	binder	244:249	arg1	atomization					251:261	binder atomization	244:261	binder atomization in pharmaceutical fluidized bed granulation using an empirical model	244:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	0	9	theme	pharmaceutical	97:110	arg1	granulation					126:136	pharmaceutical fluidized bed granulation	97:136	pharmaceutical fluidized bed granulation	97:136	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	5	10	theme	droplet	1032:1038	arg1	size					1040:1043	the droplet size	1028:1043	the droplet size	1028:1043	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	4	11	theme	droplet	758:764	arg1	model					771:775	the droplet size model	754:775	the droplet size model	754:775	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	1	12	theme	atomization	251:261	arg1	process					233:239	the process	229:239	the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model	229:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	2	13	theme	central	501:507	arg1	design					519:524	a response surface central composite design	482:524	a response surface central composite design	482:524	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	2	14	theme	binder	352:357	arg1	viscosity					359:367	the binder viscosity	348:367	the binder viscosity	348:367	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	6	15	theme	study	1169:1173	arg1	significant					1180:1190	significant	1180:1190	significant	1180:1190	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	6	15	theme	study	1169:1173	arg1	results					1146:1152	The results	1142:1152	The results of the present study	1142:1173	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	2	16	theme	surface	493:499	arg1	design					519:524	a response surface central composite design	482:524	a response surface central composite design	482:524	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	5	17	theme	measured	995:1002	arg1	values					1018:1023	the measured and predicted values	991:1023	values	1018:1023	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	2	18	from	effects	337:343	arg1	size					430:433	the droplet size	418:433	the droplet size	418:433	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	2	18	from	effects	337:343	arg1	angle					452:456	the spraying angle	439:456	the spraying angle	439:456	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	6	19	theme	granulation	1289:1299	arg1	process					1249:1255	the process	1245:1255	the process of pharmaceutical fluidized bed granulation	1245:1299	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	0	20	theme	fluidized	112:120	arg1	granulation					126:136	pharmaceutical fluidized bed granulation	97:136	pharmaceutical fluidized bed granulation	97:136	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	1	21	from	granulation	295:305	arg1	process					233:239	the process	229:239	the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model	229:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	2	22	theme	droplet	422:428	arg1	size					430:433	the droplet size	418:433	the droplet size	418:433	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	0	23	theme	bed	122:124	arg1	granulation					126:136	pharmaceutical fluidized bed granulation	97:136	pharmaceutical fluidized bed granulation	97:136	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	0	24	theme	prediction	10:19	arg1	model					21:25	Empirical prediction model	0:25	Empirical prediction model	0:25	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	3	25	theme	predicted	712:720	arg1	values					722:727	the measured and predicted values	695:727	values	722:727	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	5	26	theme	angle	1062:1066	arg1	values					1018:1023	the measured and predicted values	991:1023	values	1018:1023	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	3	27	theme	Prediction	552:561	arg1	models					563:568	Prediction models	552:568	Prediction models for droplet size and spraying angle	552:604	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	5	28	theme	spraying	1053:1060	arg1	angle					1062:1066	the spraying angle	1049:1066	the spraying angle	1049:1066	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	2	29	theme	atomization	374:384	arg1	pressure					386:393	the atomization pressure	370:393	the atomization pressure	370:393	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	0	30	theme	Empirical	0:8	arg1	model					21:25	Empirical prediction model	0:25	Empirical prediction model	0:25	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	1	31	theme	pharmaceutical	266:279	arg1	granulation					295:305	pharmaceutical fluidized bed granulation	266:305	pharmaceutical fluidized bed granulation using an empirical model	266:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	6	32	theme	droplet	1210:1216	arg1	size					1218:1221	droplet size	1210:1221	droplet size	1210:1221	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	1	33	theme	droplet	186:192	arg1	size					194:197	the droplet size	182:197	the droplet size	182:197	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	1	34	theme	fluidized	281:289	arg1	granulation					295:305	pharmaceutical fluidized bed granulation	266:305	pharmaceutical fluidized bed granulation using an empirical model	266:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	1	35	from	atomization	251:261	arg1	granulation					295:305	pharmaceutical fluidized bed granulation	266:305	pharmaceutical fluidized bed granulation using an empirical model	266:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	6	36	from	angle	1236:1240	arg1	process					1249:1255	the process	1245:1255	the process of pharmaceutical fluidized bed granulation	1245:1299	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	2	37	theme	rate	410:413	arg1	effects					337:343	The effects	333:343	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle	333:456	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	1	38	theme	bed	291:293	arg1	granulation					295:305	pharmaceutical fluidized bed granulation	266:305	pharmaceutical fluidized bed granulation using an empirical model	266:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	4	39	theme	mean	872:875	arg1	RMSE					891:894	RMSE	891:894	RMSE	891:894	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	39	theme	mean	872:875	arg1	error					884:888	a root mean square error	865:888	a root mean square error (RMSE)	865:895	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	3	40	theme	stepwise	634:641	arg1	analysis					654:661	stepwise regression analysis	634:661	stepwise regression analysis	634:661	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	2	41	theme	spray	404:408	arg1	rate					410:413	the spray rate	400:413	the spray rate	400:413	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	4	42	theme	0.82	920:923	arg1	RMSE					945:948	an RMSE	942:948	an RMSE of 3.69, respectively	942:970	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	42	theme	0.82	920:923	arg1	R2					914:915	an R2	911:915	an R2 of 0.82 (p < 0.0001)	911:936	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	3	43	theme	regression	643:652	arg1	analysis					654:661	stepwise regression analysis	634:661	stepwise regression analysis	634:661	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	6	44	theme	present	1161:1167	arg1	study					1169:1173	the present study	1157:1173	the present study	1157:1173	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	0	45	theme	process	33:39	arg1	optimization					41:52	process optimization	33:52	process optimization for droplet size and spraying angle	33:88	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	5	46	theme	size	1040:1043	arg1	values					1018:1023	the measured and predicted values	991:1023	values	1018:1023	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	2	47	theme	spraying	443:450	arg1	angle					452:456	the spraying angle	439:456	the spraying angle	439:456	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	1	48	theme	spraying	207:214	arg1	angle					216:220	the spraying angle	203:220	the spraying angle	203:220	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	0	49	theme	droplet	58:64	arg1	size					66:69	droplet size	58:69	droplet size	58:69	Empirical prediction model based process optimization for droplet size and spraying angle during pharmaceutical fluidized bed granulation.					
32129125	2	50	theme	composite	509:517	arg1	design					519:524	a response surface central composite design	482:524	a response surface central composite design	482:524	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	4	51	theme	error	884:888	arg1	R2					837:838	an R2	834:838	an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10	834:904	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	1	52	theme	empirical	316:324	arg1	model					326:330	an empirical model	313:330	an empirical model	313:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	1	53	from	process	233:239	arg1	granulation					295:305	pharmaceutical fluidized bed granulation	266:305	pharmaceutical fluidized bed granulation using an empirical model	266:330	The objective of this study was to predict the droplet size and the spraying angle during the process of binder atomization in pharmaceutical fluidized bed granulation using an empirical model.					
32129125	3	54	theme	droplet	574:580	arg1	size					582:585	droplet size	574:585	droplet size	574:585	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	6	55	dep	size	1218:1221	arg1	the					1206:1208	the	1206:1208	the	1206:1208	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	4	56	theme	10.10	900:904	arg1	R2					837:838	an R2	834:838	an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10	834:904	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	57	theme	angle	794:798	arg1	model					800:804	the spraying angle model	781:804	the spraying angle model	781:804	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	58	theme	root	867:870	arg1	RMSE					891:894	RMSE	891:894	RMSE	891:894	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	58	theme	root	867:870	arg1	error					884:888	a root mean square error	865:888	a root mean square error (RMSE)	865:895	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	2	59	theme	response	484:491	arg1	design					519:524	a response surface central composite design	482:524	a response surface central composite design	482:524	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	2	60	theme	variance	542:549	arg1	analysis					530:537	analysis	530:537	analysis of variance	530:549	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	2	60	theme	variance	542:549	arg1	design					519:524	a response surface central composite design	482:524	a response surface central composite design	482:524	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	6	61	theme	pharmaceutical	1260:1273	arg1	granulation					1289:1299	pharmaceutical fluidized bed granulation	1260:1299	pharmaceutical fluidized bed granulation	1260:1299	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	4	62	theme	square	877:882	arg1	RMSE					891:894	RMSE	891:894	RMSE	891:894	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	62	theme	square	877:882	arg1	error					884:888	a root mean square error	865:888	a root mean square error (RMSE)	865:895	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	3	63	theme	spraying	591:598	arg1	angle					600:604	spraying angle	591:604	spraying angle	591:604	Prediction models for droplet size and spraying angle were then established using stepwise regression analysis and were validated by comparing the measured and predicted values.					
32129125	4	64	dep	established	816:826	arg1	R2					914:915	an R2	911:915	an R2 of 0.82 (p < 0.0001)	911:936	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	64	dep	established	816:826	arg1	RMSE					945:948	an RMSE	942:948	an RMSE of 3.69, respectively	942:970	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	6	65	theme	fluidized	1275:1283	arg1	granulation					1289:1299	pharmaceutical fluidized bed granulation	1260:1299	pharmaceutical fluidized bed granulation	1260:1299	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	2	66	theme	pressure	386:393	arg1	effects					337:343	The effects	333:343	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle	333:456	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	5	67	theme	established	1108:1118	arg1	accurate					1132:1139	accurate	1132:1139	accurate	1132:1139	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	5	67	theme	established	1108:1118	arg1	models					1120:1125	the established models	1104:1125	the established models	1104:1125	The error between the measured and predicted values of the droplet size and the spraying angle were less than 10%, indicating that the established models were accurate.					
32129125	6	68	theme	bed	1285:1287	arg1	granulation					1289:1299	pharmaceutical fluidized bed granulation	1260:1299	pharmaceutical fluidized bed granulation	1260:1299	The results of the present study were significant in predicting the droplet size and spraying angle in the process of pharmaceutical fluidized bed granulation.					
32129125	4	69	theme	spraying	785:792	arg1	model					800:804	the spraying angle model	781:804	the spraying angle model	781:804	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	2	70	theme	viscosity	359:367	arg1	effects					337:343	The effects	333:343	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle	333:456	The effects of the binder viscosity, the atomization pressure, and the spray rate on the droplet size and the spraying angle were investigated using a response surface central composite design and analysis of variance.					
32129125	4	71	theme	3.69	953:956	arg1	RMSE					945:948	an RMSE	942:948	an RMSE of 3.69, respectively	942:970	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
32129125	4	71	theme	3.69	953:956	arg1	R2					914:915	an R2	911:915	an R2 of 0.82 (p < 0.0001)	911:936	The results showed that the droplet size model and the spraying angle model were well established, with an R2 of 0.93 (p < 0.0001) and a root mean square error (RMSE) of 10.10, and an R2 of 0.82 (p < 0.0001) and an RMSE of 3.69, respectively.					
33779853	8	0	theme	scaffold	1445:1452	arg1	construction					1397:1408	the in vitro construction	1384:1408	the in vitro construction of a human-derived WJ-CS composite scaffold	1384:1452	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	2	1	theme	composite	554:562	arg1	scaffolds					564:572	the biomimetic composite scaffolds	539:572	the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS	539:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	2	theme	biomechanical	649:661	arg1	properties					663:672	the biomechanical properties	645:672	the biomechanical properties of both the composite scaffold and the WJ scaffold	645:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	3	3	theme	composite	754:762	arg1	scaffolds					764:772	composite scaffolds	754:772	composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs	754:829	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	8	4	theme	in	1388:1389	arg1	construction					1397:1408	the in vitro construction	1384:1408	the in vitro construction of a human-derived WJ-CS composite scaffold	1384:1452	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	2	5	theme	biomimetic	543:552	arg1	scaffolds					564:572	the biomimetic composite scaffolds	539:572	the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS	539:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	3	6	theme	WJ	798:799	arg1	scaffolds					801:809	WJ scaffolds	798:809	WJ scaffolds	798:809	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	5	7	theme	scaffold	1102:1109	arg1	modulus					1077:1083	the Young's modulus	1065:1083	the Young's modulus of the composite scaffold (WJ-CS)	1065:1117	We found that in vitro, the Young's modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold.					
33779853	0	8	theme	umbilical	83:91	arg1	cells					115:119	human umbilical cord mesenchymal stem cells	77:119	human umbilical cord mesenchymal stem cells	77:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	8	9	theme	WJ-CS	1429:1433	arg1	scaffold					1445:1452	a human-derived WJ-CS composite scaffold	1413:1452	a human-derived WJ-CS composite scaffold	1413:1452	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	8	10	from	rats	1547:1550	arg1	repair					1511:1516	the repair	1507:1516	the repair of knee cartilage defects in rats	1507:1550	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	8	10	from	rats	1547:1550	arg1	better					1555:1560	better	1555:1560	better	1555:1560	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	5	11	theme	WJ	1147:1148	arg1	scaffold					1150:1157	the WJ scaffold	1143:1157	the WJ scaffold	1143:1157	We found that in vitro, the Young's modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold.					
33779853	0	12	from	defects	149:155	arg1	knee					164:167	rat knee	160:167	rat knee	160:167	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	0	13	theme	human	77:81	arg1	cells					115:119	human umbilical cord mesenchymal stem cells	77:119	human umbilical cord mesenchymal stem cells	77:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	1	14	theme	composite	297:305	arg1	scaffold					307:314	the composite scaffold	293:314	the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs)	293:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	2	15	theme	chemical	594:601	arg1	crosslinking					603:614	the physical and chemical crosslinking	577:614	the physical and chemical crosslinking of WJ and CS	577:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	0	16	theme	mesenchymal	98:108	arg1	cells					115:119	human umbilical cord mesenchymal stem cells	77:119	human umbilical cord mesenchymal stem cells	77:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	1	17	theme	scaffold	307:314	arg1	performance					186:196	the performance	182:196	the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS)	182:273	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	17	theme	scaffold	307:314	arg1	effect					283:288	the effect	279:288	the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs)	279:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	2	18	theme	physical	581:588	arg1	crosslinking					603:614	the physical and chemical crosslinking	577:614	the physical and chemical crosslinking of WJ and CS	577:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	1	19	theme	articular	395:403	arg1	defects					415:421	articular cartilage defects	395:421	articular cartilage defects	395:421	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	8	20	from	defects	1536:1542	arg1	rats					1547:1550	rats	1547:1550	rats	1547:1550	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	2	21	theme	scaffold	696:703	arg1	properties					663:672	the biomechanical properties	645:672	the biomechanical properties of both the composite scaffold and the WJ scaffold	645:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	0	22	theme	cord	93:96	arg1	cells					115:119	human umbilical cord mesenchymal stem cells	77:119	human umbilical cord mesenchymal stem cells	77:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	5	23	theme	composite	1092:1100	arg1	WJ-CS					1112:1116	WJ-CS	1112:1116	WJ-CS	1112:1116	We found that in vitro, the Young's modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold.					
33779853	5	23	theme	composite	1092:1100	arg1	scaffold					1102:1109	the composite scaffold	1088:1109	the composite scaffold (WJ-CS)	1088:1117	We found that in vitro, the Young's modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold.					
33779853	1	24	theme	cartilage	405:413	arg1	defects					415:421	articular cartilage defects	395:421	articular cartilage defects	395:421	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	8	25	link	human-derived	1415:1427	arg1	scaffold					1445:1452	a human-derived WJ-CS composite scaffold	1413:1452	a human-derived WJ-CS composite scaffold	1413:1452	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	2	26	theme	composite	686:694	arg1	scaffold					696:703	the composite scaffold	682:703	the composite scaffold	682:703	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	6	27	theme	cartilage	1225:1233	arg1	defects					1235:1241	rat cartilage defects	1221:1241	rat cartilage defects	1221:1241	In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs.					
33779853	0	28	theme	composite	2:10	arg1	scaffold					12:19	A composite scaffold	0:19	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells	0:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	2	29	theme	CS	626:627	arg1	crosslinking					603:614	the physical and chemical crosslinking	577:614	the physical and chemical crosslinking of WJ and CS	577:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	8	30	theme	WJ	1495:1496	arg1	properties					1481:1490	the biomechanical properties	1463:1490	the biomechanical properties of WJ	1463:1496	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	6	31	theme	composite	1173:1181	arg1	scaffold					1183:1190	the composite scaffold	1169:1190	the composite scaffold loaded with hUCMSCs	1169:1210	In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs.					
33779853	7	32	theme	low	1333:1335	arg1	immunogenicity					1337:1350	low immunogenicity	1333:1350	low immunogenicity	1333:1350	Both the scaffold and hUCMSCs showed low immunogenicity.					
33779853	2	33	theme	scaffolds	564:572	arg1	identification					492:505	identification	492:505	identification of the hUCMSCs	492:520	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	33	theme	scaffolds	564:572	arg1	testing					634:640	testing	634:640	testing of the biomechanical properties of both the composite scaffold and the WJ scaffold	634:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	33	theme	scaffolds	564:572	arg1	construction					523:534	construction	523:534	construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS	523:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	8	34	from	repair	1511:1516	arg1	rats					1547:1550	rats	1547:1550	rats	1547:1550	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	6	35	dep	did	1255:1257	arg1	than					1250:1253	than	1250:1253	than	1250:1253	In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs.					
33779853	3	36	theme	cartilage	864:872	arg1	defects					874:880	articular cartilage defects	854:880	articular cartilage defects in the rat knee	854:896	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	8	37	theme	human-derived	1415:1427	arg1	scaffold					1445:1452	a human-derived WJ-CS composite scaffold	1413:1452	a human-derived WJ-CS composite scaffold	1413:1452	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	2	38	theme	WJ	619:620	arg1	crosslinking					603:614	the physical and chemical crosslinking	577:614	the physical and chemical crosslinking of WJ and CS	577:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	39	theme	scaffold	716:723	arg1	properties					663:672	the biomechanical properties	645:672	the biomechanical properties of both the composite scaffold and the WJ scaffold	645:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	4	40	theme	unaided	952:958	arg1	eye					960:962	the unaided eye	948:962	the unaided eye	948:962	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	1	41	theme	chondroitin	250:260	arg1	CS					271:272	CS	271:272	CS	271:272	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	41	theme	chondroitin	250:260	arg1	sulfate					262:268	chondroitin sulfate	250:268	chondroitin sulfate (CS)	250:273	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	0	42	theme	stem	110:113	arg1	cells					115:119	human umbilical cord mesenchymal stem cells	77:119	human umbilical cord mesenchymal stem cells	77:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	1	43	theme	human	328:332	arg1	cells					366:370	human umbilical cord mesenchymal stem cells	328:370	human umbilical cord mesenchymal stem cells (hUCMSCs)	328:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	43	theme	human	328:332	arg1	hUCMSCs					373:379	hUCMSCs	373:379	hUCMSCs	373:379	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	4	44	theme	hUCMSCs	1032:1038	arg1	observations					978:989	histological observations	965:989	histological observations	965:989	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	4	44	theme	hUCMSCs	1032:1038	arg1	immunogenicity					1000:1013	the immunogenicity	996:1013	the immunogenicity of scaffolds and hUCMSCs	996:1038	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	4	44	theme	hUCMSCs	1032:1038	arg1	eye					960:962	the unaided eye	948:962	the unaided eye	948:962	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	2	45	theme	WJ	713:714	arg1	scaffold					716:723	the WJ scaffold	709:723	the WJ scaffold	709:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	8	46	theme	single	1604:1609	arg1	scaffold					1614:1621	the single WJ scaffold	1600:1621	the single WJ scaffold	1600:1621	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	8	47	theme	composite	1435:1443	arg1	scaffold					1445:1452	a human-derived WJ-CS composite scaffold	1413:1452	a human-derived WJ-CS composite scaffold	1413:1452	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	0	48	theme	articular	129:137	arg1	defects					149:155	articular cartilage defects	129:155	articular cartilage defects in rat knee	129:167	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	1	49	theme	umbilical	334:342	arg1	cells					366:370	human umbilical cord mesenchymal stem cells	328:370	human umbilical cord mesenchymal stem cells (hUCMSCs)	328:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	49	theme	umbilical	334:342	arg1	hUCMSCs					373:379	hUCMSCs	373:379	hUCMSCs	373:379	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	8	50	theme	WJ	1611:1612	arg1	scaffold					1614:1621	the single WJ scaffold	1600:1621	the single WJ scaffold	1600:1621	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	3	51	theme	articular	854:862	arg1	defects					874:880	articular cartilage defects	854:880	articular cartilage defects in the rat knee	854:896	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	4	52	theme	repair	915:920	arg1	effects					922:928	their repair effects	909:928	their repair effects	909:928	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	2	53	dep	in	462:463	arg1	vitro					465:469	vitro	465:469	vitro	465:469	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	1	54	theme	cord	344:347	arg1	cells					366:370	human umbilical cord mesenchymal stem cells	328:370	human umbilical cord mesenchymal stem cells (hUCMSCs)	328:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	54	theme	cord	344:347	arg1	hUCMSCs					373:379	hUCMSCs	373:379	hUCMSCs	373:379	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	3	55	theme	rat	889:891	arg1	knee					893:896	the rat knee	885:896	the rat knee	885:896	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	6	56	theme	rat	1221:1223	arg1	defects					1235:1241	rat cartilage defects	1221:1241	rat cartilage defects	1221:1241	In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs.					
33779853	1	57	theme	mesenchymal	349:359	arg1	cells					366:370	human umbilical cord mesenchymal stem cells	328:370	human umbilical cord mesenchymal stem cells (hUCMSCs)	328:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	57	theme	mesenchymal	349:359	arg1	hUCMSCs					373:379	hUCMSCs	373:379	hUCMSCs	373:379	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	8	58	theme	knee	1521:1524	arg1	defects					1536:1542	knee cartilage defects	1521:1542	knee cartilage defects in rats	1521:1550	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	2	59	theme	hUCMSCs	514:520	arg1	identification					492:505	identification	492:505	identification of the hUCMSCs	492:520	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	59	theme	hUCMSCs	514:520	arg1	testing					634:640	testing	634:640	testing of the biomechanical properties of both the composite scaffold and the WJ scaffold	634:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	59	theme	hUCMSCs	514:520	arg1	construction					523:534	construction	523:534	construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS	523:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	0	60	theme	jelly	34:38	arg1	scaffold					12:19	A composite scaffold	0:19	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells	0:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	0	61	theme	cartilage	139:147	arg1	defects					149:155	articular cartilage defects	129:155	articular cartilage defects in rat knee	129:167	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	1	62	theme	stem	361:364	arg1	cells					366:370	human umbilical cord mesenchymal stem cells	328:370	human umbilical cord mesenchymal stem cells (hUCMSCs)	328:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	62	theme	stem	361:364	arg1	hUCMSCs					373:379	hUCMSCs	373:379	hUCMSCs	373:379	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	3	63	from	defects	874:880	arg1	knee					893:896	the rat knee	885:896	the rat knee	885:896	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	3	64	dep	in	733:734	arg1	vivo					736:739	vivo	736:739	vivo	736:739	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	8	65	theme	defects	1536:1542	arg1	repair					1511:1516	the repair	1507:1516	the repair of knee cartilage defects in rats	1507:1550	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	8	65	theme	defects	1536:1542	arg1	better					1555:1560	better	1555:1560	better	1555:1560	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	8	66	theme	biomechanical	1467:1479	arg1	properties					1481:1490	the biomechanical properties	1463:1490	the biomechanical properties of WJ	1463:1496	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	0	67	theme	rat	160:162	arg1	knee					164:167	rat knee	160:167	rat knee	160:167	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	8	68	theme	cartilage	1526:1534	arg1	defects					1536:1542	knee cartilage defects	1521:1542	knee cartilage defects in rats	1521:1550	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	1	69	theme	composite	203:211	arg1	scaffold					213:220	a composite scaffold	201:220	a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS)	201:273	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	6	70	theme	WJ	1263:1264	arg1	scaffold					1266:1273	the WJ scaffold	1259:1273	the WJ scaffold loaded with hUCMSCs	1259:1293	In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs.					
33779853	4	71	theme	scaffolds	1018:1026	arg1	observations					978:989	histological observations	965:989	histological observations	965:989	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	4	71	theme	scaffolds	1018:1026	arg1	immunogenicity					1000:1013	the immunogenicity	996:1013	the immunogenicity of scaffolds and hUCMSCs	996:1038	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	4	71	theme	scaffolds	1018:1026	arg1	eye					960:962	the unaided eye	948:962	the unaided eye	948:962	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	8	72	theme	composite	1571:1579	arg1	scaffold					1581:1588	the composite scaffold	1567:1588	the composite scaffold	1567:1588	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	1	73	theme	scaffold	213:220	arg1	performance					186:196	the performance	182:196	the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS)	182:273	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	1	73	theme	scaffold	213:220	arg1	effect					283:288	the effect	279:288	the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs)	279:380	To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out.					
33779853	0	74	theme	sulphate	56:63	arg1	scaffold					12:19	A composite scaffold	0:19	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells	0:119	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	8	75	dep	in	1388:1389	arg1	vitro					1391:1395	vitro	1391:1395	vitro	1391:1395	These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.					
33779853	0	76	theme	chondroitin	44:54	arg1	sulphate					56:63	chondroitin sulphate	44:63	chondroitin sulphate	44:63	A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee.					
33779853	2	77	theme	properties	663:672	arg1	identification					492:505	identification	492:505	identification of the hUCMSCs	492:520	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	77	theme	properties	663:672	arg1	testing					634:640	testing	634:640	testing of the biomechanical properties of both the composite scaffold and the WJ scaffold	634:723	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	2	77	theme	properties	663:672	arg1	construction					523:534	construction	523:534	construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS	523:627	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33779853	3	78	theme	in	733:734	arg1	experiments					741:751	the in vivo experiments	729:751	the in vivo experiments	729:751	In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee.					
33779853	4	79	theme	histological	965:976	arg1	observations					978:989	histological observations	965:989	histological observations	965:989	Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs.					
33779853	2	80	theme	in	462:463	arg1	experiments					471:481	The in vitro experiments	458:481	The in vitro experiments	458:481	The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold.					
33516853	5	0	theme	Ag	1068:1069	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	0	theme	Ag	1068:1069	arg1	APs					1080:1082	Ag@CS/PMS-4 APs	1068:1082	Ag@CS/PMS-4 APs	1068:1082	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	0	1	theme	antibacterial	69:81	arg1	activity					83:90	high antibacterial activity	64:90	high antibacterial activity	64:90	Synthesis of Ag@chitosan/copolymer with dual-active centers for high antibacterial activity.					
33516853	5	2	theme	@	1070:1070	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	2	theme	@	1070:1070	arg1	APs					1080:1082	Ag@CS/PMS-4 APs	1068:1082	Ag@CS/PMS-4 APs	1068:1082	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	2	3	theme	methacryloylamino	354:370	arg1	chloride					398:405	3-(methacryloylamino) propyltrimethyl ammonium chloride	351:405	3-(methacryloylamino) propyltrimethyl ammonium chloride	351:405	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	3	theme	methacryloylamino	354:370	arg1	poly					346:349	poly	346:349	poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS)	346:428	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	5	4	theme	resulting	964:972	arg1	series					974:979	the resulting series	960:979	the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	960:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	5	theme	PMS	700:702	arg1	APs					704:706	PMS APs	700:706	PMS APs	700:706	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	5	6	theme	APs	1047:1049	arg1	film					1061:1064	Ag@CS/PMS-4 APs composite film	1035:1064	Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	1035:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	6	theme	APs	1047:1049	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	2	7	theme	ammonium	311:318	arg1	groups					320:325	antibacterial quaternary ammonium groups	286:325	antibacterial quaternary ammonium groups	286:325	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	7	theme	ammonium	311:318	arg1	groups					338:343	pendant groups	330:343	pendant groups	330:343	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	8	contain	containing	275:284	arg2	groups					320:325	antibacterial quaternary ammonium groups	286:325	antibacterial quaternary ammonium groups	286:325	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	8	contain	containing	275:284	arg1	poly					346:349	poly	346:349	poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS)	346:428	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	8	contain	containing	275:284	arg2	groups					338:343	pendant groups	330:343	pendant groups	330:343	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	8	contain	containing	275:284	arg1	copolymer					265:273	a novel diblock copolymer	249:273	a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups	249:343	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	4	9	theme	composite	927:935	arg1	materials					937:945	composite materials	927:945	composite materials	927:945	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	10	theme	@	691:691	arg1	chitosan					679:686	silver loaded chitosan	665:686	silver loaded chitosan (Ag@CS)	665:694	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	10	theme	@	691:691	arg1	CS					692:693	Ag@CS	689:693	Ag@CS	689:693	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	2	11	theme	quaternary	300:309	arg1	ammonium					311:318	antibacterial quaternary ammonium	286:318	antibacterial quaternary ammonium groups	286:325	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	5	12	theme	CS/PMS-4	1038:1045	arg1	film					1061:1064	Ag@CS/PMS-4 APs composite film	1035:1064	Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	1035:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	12	theme	CS/PMS-4	1038:1045	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	13	theme	film	1061:1064	arg1	series					974:979	the resulting series	960:979	the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	960:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	14	theme	@	811:811	arg1	APs					821:823	Ag@CS/PMS-4 APs	809:823	Ag@CS/PMS-4 APs	809:823	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	14	theme	@	811:811	arg1	centers					800:806	dual-active centers	788:806	dual-active centers (Ag@CS/PMS-4 APs)	788:824	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	2	15	theme	antibacterial	286:298	arg1	ammonium					311:318	antibacterial quaternary ammonium	286:318	antibacterial quaternary ammonium groups	286:325	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	3	16	theme	different	556:564	arg1	behaviors					575:583	different assembly behaviors	556:583	different assembly behaviors	556:583	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	5	17	theme	composite	1051:1059	arg1	film					1061:1064	Ag@CS/PMS-4 APs composite film	1035:1064	Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	1035:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	17	theme	composite	1051:1059	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	1	18	theme	significant	199:209	arg1	concern					211:217	significant concern	199:217	significant concern of scientists	199:231	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	1	19	from	treatment	112:120	arg1	surfaces					166:173	substrate surfaces	156:173	substrate surfaces	156:173	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	3	20	theme	assembly	566:573	arg1	behaviors					575:583	different assembly behaviors	556:583	different assembly behaviors	556:583	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	0	21	theme	@	15:15	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Ag@	0:15	Synthesis of Ag@chitosan/copolymer with dual-active centers for high antibacterial activity.					
33516853	5	22	theme	excellent	1098:1106	arg1	materials					1169:1177	interface materials	1159:1177	interface materials	1159:1177	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	22	theme	excellent	1098:1106	arg1	properties					1122:1131	excellent antibacterial properties	1098:1131	excellent antibacterial properties	1098:1131	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	23	theme	microorganism	1240:1252	arg1	contamination					1254:1266	microorganism contamination	1240:1266	microorganism contamination	1240:1266	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	2	24	theme	pendant	330:336	arg1	groups					320:325	antibacterial quaternary ammonium groups	286:325	antibacterial quaternary ammonium groups	286:325	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	24	theme	pendant	330:336	arg1	groups					338:343	pendant groups	330:343	pendant groups	330:343	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	1	25	dep	prevention	97:106	arg1	The					93:95	The	93:95	The	93:95	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	0	26	theme	Ag	13:14	arg1	@					15:15	Ag@	13:15	Ag@	13:15	Synthesis of Ag@chitosan/copolymer with dual-active centers for high antibacterial activity.					
33516853	5	27	theme	antibacterial	1108:1120	arg1	materials					1169:1177	interface materials	1159:1177	interface materials	1159:1177	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	27	theme	antibacterial	1108:1120	arg1	properties					1122:1131	excellent antibacterial properties	1098:1131	excellent antibacterial properties	1098:1131	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	28	theme	polymers	876:883	arg1	application					848:858	the application	844:858	the application of carbohydrate polymers	844:883	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	1	29	theme	scientists	222:231	arg1	concern					211:217	significant concern	199:217	significant concern of scientists	199:231	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	4	30	theme	Ag	689:690	arg1	chitosan					679:686	silver loaded chitosan	665:686	silver loaded chitosan (Ag@CS)	665:694	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	30	theme	Ag	689:690	arg1	CS					692:693	Ag@CS	689:693	Ag@CS	689:693	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	5	31	theme	Ag	1035:1036	arg1	film					1061:1064	Ag@CS/PMS-4 APs composite film	1035:1064	Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	1035:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	31	theme	Ag	1035:1036	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	32	theme	PMS	984:986	arg1	particles					988:996	PMS particles	984:996	PMS particles	984:996	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	33	theme	loaded	672:677	arg1	chitosan					679:686	silver loaded chitosan	665:686	silver loaded chitosan (Ag@CS)	665:694	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	33	theme	loaded	672:677	arg1	CS					692:693	Ag@CS	689:693	Ag@CS	689:693	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	5	34	theme	@	1037:1037	arg1	film					1061:1064	Ag@CS/PMS-4 APs composite film	1035:1064	Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	1035:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	34	theme	@	1037:1037	arg1	-F					1084:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	(Ag@CS/PMS-4 APs)-F	1067:1085	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	35	theme	dual-active	788:798	arg1	APs					821:823	Ag@CS/PMS-4 APs	809:823	Ag@CS/PMS-4 APs	809:823	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	35	theme	dual-active	788:798	arg1	centers					800:806	dual-active centers	788:806	dual-active centers (Ag@CS/PMS-4 APs)	788:824	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	2	36	theme	interfacial	450:460	arg1	polymerization					462:475	interfacial polymerization	450:475	interfacial polymerization	450:475	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	5	37	theme	PMS-4	1020:1024	arg1	APs					1026:1028	especially worm-like PMS-4 APs	999:1028	especially worm-like PMS-4 APs	999:1028	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	38	theme	Ag	809:810	arg1	APs					821:823	Ag@CS/PMS-4 APs	809:823	Ag@CS/PMS-4 APs	809:823	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	38	theme	Ag	809:810	arg1	centers					800:806	dual-active centers	788:806	dual-active centers (Ag@CS/PMS-4 APs)	788:824	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	3	39	theme	PMS	484:486	arg1	APs					511:513	APs	511:513	APs	511:513	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	3	39	theme	PMS	484:486	arg1	particles					500:508	PMS anisotropic particles	484:508	PMS anisotropic particles (APs)	484:514	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	0	40	theme	dual-active	40:50	arg1	centers					52:58	dual-active centers	40:58	dual-active centers for high antibacterial activity	40:90	Synthesis of Ag@chitosan/copolymer with dual-active centers for high antibacterial activity.					
33516853	5	41	theme	worm-like	1010:1018	arg1	APs					1026:1028	especially worm-like PMS-4 APs	999:1028	especially worm-like PMS-4 APs	999:1028	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	1	42	theme	substrate	156:164	arg1	surfaces					166:173	substrate surfaces	156:173	substrate surfaces	156:173	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	3	43	theme	anisotropic	488:498	arg1	APs					511:513	APs	511:513	APs	511:513	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	3	43	theme	anisotropic	488:498	arg1	particles					500:508	PMS anisotropic particles	484:508	PMS anisotropic particles (APs)	484:514	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	1	44	from	prevention	97:106	arg1	surfaces					166:173	substrate surfaces	156:173	substrate surfaces	156:173	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	4	45	theme	carbohydrate	863:874	arg1	polymers					876:883	carbohydrate polymers	863:883	carbohydrate polymers	863:883	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	4	46	theme	antibacterial	759:771	arg1	materials					773:781	natural/synthetic polymer antibacterial materials	733:781	natural/synthetic polymer antibacterial materials	733:781	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	3	47	theme	monomers	612:619	arg1	Tol/St					646:651	Tol/St	646:651	Tol/St	646:651	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	3	47	theme	monomers	612:619	arg1	toluene/styrene					629:643	the toluene/styrene	625:643	the toluene/styrene (Tol/St)	625:652	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	3	47	theme	monomers	612:619	arg1	ratios					602:607	the ratios	598:607	the ratios of monomers	598:619	Also, PMS anisotropic particles (APs) could be successfully obtained based on different assembly behaviors by adjusting the ratios of monomers and the toluene/styrene (Tol/St).					
33516853	5	48	theme	particles	988:996	arg1	series					974:979	the resulting series	960:979	the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	960:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	1	49	theme	microorganism	125:137	arg1	contamination					139:151	microorganism contamination	125:151	microorganism contamination	125:151	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	5	50	theme	infectious	1210:1219	arg1	diseases					1221:1228	infectious diseases	1210:1228	infectious diseases caused by microorganism contamination	1210:1266	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	51	theme	natural/synthetic	733:749	arg1	materials					773:781	natural/synthetic polymer antibacterial materials	733:781	natural/synthetic polymer antibacterial materials	733:781	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	5	52	theme	APs	1026:1028	arg1	series					974:979	the resulting series	960:979	the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F)	960:1086	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	53	theme	polymer	751:757	arg1	materials					773:781	natural/synthetic polymer antibacterial materials	733:781	natural/synthetic polymer antibacterial materials	733:781	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	1	54	theme	contamination	139:151	arg1	treatment					112:120	treatment	112:120	treatment	112:120	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	1	54	theme	contamination	139:151	arg1	prevention					97:106	prevention	97:106	prevention	97:106	The prevention and treatment of microorganism contamination on substrate surfaces have recently generated significant concern of scientists.					
33516853	5	55	theme	diseases	1221:1228	arg1	transmission					1194:1205	the transmission	1190:1205	the transmission of infectious diseases caused by microorganism contamination	1190:1266	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	0	56	theme	high	64:67	arg1	activity					83:90	high antibacterial activity	64:90	high antibacterial activity	64:90	Synthesis of Ag@chitosan/copolymer with dual-active centers for high antibacterial activity.					
33516853	2	57	theme	diblock	257:263	arg1	poly					346:349	poly	346:349	poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS)	346:428	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	57	theme	diblock	257:263	arg1	copolymer					265:273	a novel diblock copolymer	249:273	a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups	249:343	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	58	theme	ammonium	389:396	arg1	chloride					398:405	3-(methacryloylamino) propyltrimethyl ammonium chloride	351:405	3-(methacryloylamino) propyltrimethyl ammonium chloride	351:405	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	58	theme	ammonium	389:396	arg1	poly					346:349	poly	346:349	poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS)	346:428	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	5	59	theme	interface	1159:1167	arg1	properties					1122:1131	excellent antibacterial properties	1098:1131	excellent antibacterial properties	1098:1131	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	5	59	theme	interface	1159:1167	arg1	materials					1169:1177	interface materials	1159:1177	interface materials	1159:1177	Remarkably, the resulting series of PMS particles, especially worm-like PMS-4 APs, and Ag@CS/PMS-4 APs composite film ((Ag@CS/PMS-4 APs)-F) exhibited excellent antibacterial properties, which can be employed as interface materials to prevent the transmission of infectious diseases caused by microorganism contamination.					
33516853	4	60	theme	materials	937:945	arg1	activity					915:922	the antibacterial activity	897:922	the antibacterial activity of composite materials	897:945	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33516853	2	61	theme	novel	251:255	arg1	poly					346:349	poly	346:349	poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS)	346:428	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	61	theme	novel	251:255	arg1	copolymer					265:273	a novel diblock copolymer	249:273	a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups	249:343	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	62	theme	propyltrimethyl	373:387	arg1	chloride					398:405	3-(methacryloylamino) propyltrimethyl ammonium chloride	351:405	3-(methacryloylamino) propyltrimethyl ammonium chloride	351:405	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	2	62	theme	propyltrimethyl	373:387	arg1	poly					346:349	poly	346:349	poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS)	346:428	In this paper, a novel diblock copolymer containing antibacterial quaternary ammonium groups as pendant groups, poly(3-(methacryloylamino) propyltrimethyl ammonium chloride)-b-poly(styrene) (PMS), was synthesized by interfacial polymerization.					
33516853	4	63	theme	antibacterial	901:913	arg1	activity					915:922	the antibacterial activity	897:922	the antibacterial activity of composite materials	897:945	Moreover, silver loaded chitosan (Ag@CS) and PMS APs were combined to prepare natural/synthetic polymer antibacterial materials with dual-active centers (Ag@CS/PMS-4 APs), aiming to expand the application of carbohydrate polymers and improve the antibacterial activity of composite materials.					
33376326	8	0	theme	psoriasis	1185:1193	arg1	vulgaris					1195:1202	psoriasis vulgaris	1185:1202	psoriasis vulgaris	1185:1202	The therapeutic effect of ECPN on psoriasis vulgaris was explored by animal experiments and characterizing histomorphology in vivo.					
33376326	5	1	theme	Molecular	670:678	arg1	weight					680:685	Molecular weight	670:685	Molecular weight	670:685	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	3	2	theme	poor	534:537	arg1	permeability					539:550	the poor permeability	530:550	the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris	530:610	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	13	3	theme	sustained-release	1865:1881	arg1	effect					1883:1888	a sustained-release effect	1863:1888	a sustained-release effect	1863:1888	The prepared ECPN have high stability with a sustained-release effect.					
33376326	3	4	from	permeability	539:550	arg1	skin					576:579	skin	576:579	skin affected by psoriasis vulgaris	576:610	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	3	5	with	nanoemulsions	444:456	arg1	EPS					470:472	EPS	470:472	EPS as emulsifiers	470:487	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	11	6	theme	emulsifying	1626:1636	arg1	ability					1638:1644	enhanced emulsifying ability	1617:1644	enhanced emulsifying ability	1617:1644	The EPS can form nanoparticles at pH = 4-6 with enhanced emulsifying ability.					
33376326	2	7	theme	emulsifying	293:303	arg1	activity					305:312	stronger emulsifying activity	284:312	stronger emulsifying activity	284:312	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	1	8	theme	significant	192:202	arg1	activity					216:223	significant emulsifying activity	192:223	significant emulsifying activity	192:223	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	6	9	theme	emulsifying	925:935	arg1	activity					937:944	the emulsifying activity	921:944	the emulsifying activity was studied at different pH. ECPN	921:978	EPS nanoparticles were prepared by adjusting the pH, and the emulsifying activity was studied at different pH. ECPN were prepared by ultrasound and optimized by the response surface method.					
33376326	13	10	theme	high	1843:1846	arg1	stability					1848:1856	high stability	1843:1856	high stability	1843:1856	The prepared ECPN have high stability with a sustained-release effect.					
33376326	7	11	theme	ECPN	1132:1135	arg1	stability					1093:1101	stability	1093:1101	stability	1093:1101	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	7	11	theme	ECPN	1132:1135	arg1	microstructure					1077:1090	microstructure	1077:1090	microstructure	1077:1090	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	7	11	theme	ECPN	1132:1135	arg1	distribution					1063:1074	The size distribution	1054:1074	The size distribution	1054:1074	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	7	11	theme	ECPN	1132:1135	arg1	release					1121:1127	in vitro drug release	1107:1127	in vitro drug release	1107:1127	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	2	12	theme	nanoparticles	329:341	arg1	form					321:324	the form	317:324	the form of nanoparticles	317:341	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	10	13	with	heteropolysaccharides	1395:1415	arg1	network					1442:1448	a three-dimensional network	1422:1448	a three-dimensional network composed of glucose, galactose, glucuronic acid, rhamnose, galacturonic acid and mannose (32.0:34.3:9.7:7.4:10.3:6.3)	1422:1566	EPS produced by the strain were heteropolysaccharides with a three-dimensional network composed of glucose, galactose, glucuronic acid, rhamnose, galacturonic acid and mannose (32.0:34.3:9.7:7.4:10.3:6.3).					
33376326	4	14	theme	FYS	650:652	arg1	strain					623:628	A strain	621:628	METHODS A strain of marine bacterium FYS	613:652	METHODS A strain of marine bacterium FYS was identified.					
33376326	5	15	theme	EPS	737:739	arg1	microstructure					719:732	microstructure	719:732	microstructure	719:732	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	5	15	theme	EPS	737:739	arg1	weight					680:685	Molecular weight	670:685	Molecular weight	670:685	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	5	15	theme	EPS	737:739	arg1	composition					703:713	monosaccharide composition	688:713	monosaccharide composition	688:713	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	4	16	dep	METHODS	613:619	arg1	strain					623:628	A strain	621:628	METHODS A strain of marine bacterium FYS	613:652	METHODS A strain of marine bacterium FYS was identified.					
33376326	14	17	theme	vulgaris	1992:1999	arg1	course					1972:1977	the treatment course	1958:1977	the treatment course of psoriasis vulgaris	1958:1999	Finally, animal experiments showed that ECPN effectively shortened the treatment course of psoriasis vulgaris.					
33376326	12	18	theme	EPS	1694:1696	arg1	nanoparticles					1698:1710	EPS nanoparticles	1694:1710	EPS nanoparticles	1694:1710	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	9	19	theme	phylogenetic	1293:1304	arg1	tree					1306:1309	A phylogenetic tree	1291:1309	A phylogenetic tree	1291:1309	RESULTS A phylogenetic tree revealed that FYS was a Bacillus halodurans strain.					
33376326	2	20	theme	marine	251:256	arg1	FYS					275:277	the marine bacterial strain FYS	247:277	the marine bacterial strain FYS	247:277	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	14	21	theme	treatment	1962:1970	arg1	course					1972:1977	the treatment course	1958:1977	the treatment course of psoriasis vulgaris	1958:1999	Finally, animal experiments showed that ECPN effectively shortened the treatment course of psoriasis vulgaris.					
33376326	12	22	theme	Pickering	1784:1792	arg1	mechanism					1809:1817	a Pickering emulsification mechanism	1782:1817	a Pickering emulsification mechanism	1782:1817	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	12	23	theme	electron	1660:1667	arg1	microscopy					1669:1678	Transmission electron microscopy	1647:1678	Transmission electron microscopy	1647:1678	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	1	24	theme	Many	157:160	arg1	EPS					182:184	EPS	182:184	EPS	182:184	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	1	24	theme	Many	157:160	arg1	exopolysaccharides					162:179	PURPOSE Many exopolysaccharides	149:179	PURPOSE Many exopolysaccharides (EPS)	149:185	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	5	25	theme	high-performance	791:806	arg1	chromatography					815:828	high-performance liquid chromatography	791:828	high-performance liquid chromatography	791:828	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	2	26	theme	strain	268:273	arg1	FYS					275:277	the marine bacterial strain FYS	247:277	the marine bacterial strain FYS	247:277	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	10	27	theme	galacturonic	1509:1520	arg1	acid					1522:1525	galacturonic acid	1509:1525	galacturonic acid	1509:1525	EPS produced by the strain were heteropolysaccharides with a three-dimensional network composed of glucose, galactose, glucuronic acid, rhamnose, galacturonic acid and mannose (32.0:34.3:9.7:7.4:10.3:6.3).					
33376326	15	28	theme	potential	2048:2056	arg1	mechanism					2083:2091	the potential Pickering emulsification mechanism	2044:2091	the potential Pickering emulsification mechanism	2044:2091	CONCLUSION EPS is highly possible to have the potential Pickering emulsification mechanism.					
33376326	6	29	theme	EPS	864:866	arg1	nanoparticles					868:880	EPS nanoparticles	864:880	EPS nanoparticles	864:880	EPS nanoparticles were prepared by adjusting the pH, and the emulsifying activity was studied at different pH. ECPN were prepared by ultrasound and optimized by the response surface method.					
33376326	5	30	theme	gel	760:762	arg1	chromatography					775:788	gel permeation chromatography	760:788	gel permeation chromatography	760:788	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	12	31	theme	oil	1738:1740	arg1	droplets					1742:1749	oil droplets	1738:1749	oil droplets	1738:1749	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	6	32	theme	response	1029:1036	arg1	method					1046:1051	the response surface method	1025:1051	the response surface method	1025:1051	EPS nanoparticles were prepared by adjusting the pH, and the emulsifying activity was studied at different pH. ECPN were prepared by ultrasound and optimized by the response surface method.					
33376326	0	33	theme	Therapeutic	0:10	arg1	Effect					12:17	Therapeutic Effect	0:17	Therapeutic Effect of Calcipotriol Pickering Nanoemulsions Prepared by Exopolysaccharides Produced by Bacillus halotolerans	0:122	Therapeutic Effect of Calcipotriol Pickering Nanoemulsions Prepared by Exopolysaccharides Produced by Bacillus halotolerans FYS Strain on Psoriasis.					
33376326	9	34	dep	Bacillus	1335:1342	arg1	halodurans					1344:1353	halodurans	1344:1353	halodurans	1344:1353	RESULTS A phylogenetic tree revealed that FYS was a Bacillus halodurans strain.					
33376326	5	35	theme	scanning	834:841	arg1	microscopy					852:861	scanning electron microscopy	834:861	scanning electron microscopy	834:861	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	3	36	theme	EPS/CT	427:432	arg1	ECPN					459:462	ECPN	459:462	ECPN	459:462	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	3	36	theme	EPS/CT	427:432	arg1	nanoemulsions					444:456	novel EPS/CT Pickering nanoemulsions	421:456	novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers	421:487	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	1	37	contain	have	187:190	arg1	EPS					182:184	EPS	182:184	EPS	182:184	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	1	37	contain	have	187:190	arg2	activity					216:223	significant emulsifying activity	192:223	significant emulsifying activity	192:223	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	1	37	contain	have	187:190	arg1	exopolysaccharides					162:179	PURPOSE Many exopolysaccharides	149:179	PURPOSE Many exopolysaccharides (EPS)	149:185	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	8	38	theme	therapeutic	1155:1165	arg1	effect					1167:1172	The therapeutic effect	1151:1172	The therapeutic effect of ECPN on psoriasis vulgaris	1151:1202	The therapeutic effect of ECPN on psoriasis vulgaris was explored by animal experiments and characterizing histomorphology in vivo.					
33376326	7	39	dep	in	1107:1108	arg1	vitro					1110:1114	vitro	1110:1114	vitro	1110:1114	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	10	40	theme	three-dimensional	1424:1440	arg1	network					1442:1448	a three-dimensional network	1422:1448	a three-dimensional network composed of glucose, galactose, glucuronic acid, rhamnose, galacturonic acid and mannose (32.0:34.3:9.7:7.4:10.3:6.3)	1422:1566	EPS produced by the strain were heteropolysaccharides with a three-dimensional network composed of glucose, galactose, glucuronic acid, rhamnose, galacturonic acid and mannose (32.0:34.3:9.7:7.4:10.3:6.3).					
33376326	15	41	contain	have	2039:2042	arg2	mechanism					2083:2091	the potential Pickering emulsification mechanism	2044:2091	the potential Pickering emulsification mechanism	2044:2091	CONCLUSION EPS is highly possible to have the potential Pickering emulsification mechanism.					
33376326	15	41	contain	have	2039:2042	arg1	EPS					2013:2015	CONCLUSION EPS	2002:2015	CONCLUSION EPS	2002:2015	CONCLUSION EPS is highly possible to have the potential Pickering emulsification mechanism.					
33376326	15	42	theme	emulsification	2068:2081	arg1	mechanism					2083:2091	the potential Pickering emulsification mechanism	2044:2091	the potential Pickering emulsification mechanism	2044:2091	CONCLUSION EPS is highly possible to have the potential Pickering emulsification mechanism.					
33376326	2	43	contain	have	279:282	arg1	EPS					231:233	Some EPS	226:233	Some EPS produced by the marine bacterial strain FYS	226:277	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	2	43	contain	have	279:282	arg2	activity					305:312	stronger emulsifying activity	284:312	stronger emulsifying activity	284:312	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	3	44	theme	calcipotriol	555:566	arg1	permeability					539:550	the poor permeability	530:550	the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris	530:610	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	10	45	theme	glucuronic	1482:1491	arg1	acid					1493:1496	glucuronic acid	1482:1496	glucuronic acid	1482:1496	EPS produced by the strain were heteropolysaccharides with a three-dimensional network composed of glucose, galactose, glucuronic acid, rhamnose, galacturonic acid and mannose (32.0:34.3:9.7:7.4:10.3:6.3).					
33376326	1	46	theme	emulsifying	204:214	arg1	activity					216:223	significant emulsifying activity	192:223	significant emulsifying activity	192:223	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	8	47	theme	animal	1220:1225	arg1	experiments					1227:1237	animal experiments	1220:1237	animal experiments	1220:1237	The therapeutic effect of ECPN on psoriasis vulgaris was explored by animal experiments and characterizing histomorphology in vivo.					
33376326	17	48	theme	psoriasis	2193:2201	arg1	vulgaris					2203:2210	psoriasis vulgaris	2193:2210	psoriasis vulgaris	2193:2210	ECPN also showed potential for use in the treatment of psoriasis vulgaris.					
33376326	15	49	theme	CONCLUSION	2002:2011	arg1	EPS					2013:2015	CONCLUSION EPS	2002:2015	CONCLUSION EPS	2002:2015	CONCLUSION EPS is highly possible to have the potential Pickering emulsification mechanism.					
33376326	7	50	theme	drug	1116:1119	arg1	release					1121:1127	in vitro drug release	1107:1127	in vitro drug release	1107:1127	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	11	51	theme	enhanced	1617:1624	arg1	ability					1638:1644	enhanced emulsifying ability	1617:1644	enhanced emulsifying ability	1617:1644	The EPS can form nanoparticles at pH = 4-6 with enhanced emulsifying ability.					
33376326	4	52	theme	bacterium	640:648	arg1	FYS					650:652	marine bacterium FYS	633:652	marine bacterium FYS	633:652	METHODS A strain of marine bacterium FYS was identified.					
33376326	7	53	theme	in	1107:1108	arg1	release					1121:1127	in vitro drug release	1107:1127	in vitro drug release	1107:1127	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	2	54	theme	bacterial	258:266	arg1	FYS					275:277	the marine bacterial strain FYS	247:277	the marine bacterial strain FYS	247:277	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	14	55	theme	psoriasis	1982:1990	arg1	vulgaris					1992:1999	psoriasis vulgaris	1982:1999	psoriasis vulgaris	1982:1999	Finally, animal experiments showed that ECPN effectively shortened the treatment course of psoriasis vulgaris.					
33376326	12	56	theme	Transmission	1647:1658	arg1	microscopy					1669:1678	Transmission electron microscopy	1647:1678	Transmission electron microscopy	1647:1678	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	13	57	theme	prepared	1824:1831	arg1	ECPN					1833:1836	The prepared ECPN	1820:1836	The prepared ECPN	1820:1836	The prepared ECPN have high stability with a sustained-release effect.					
33376326	5	58	theme	monosaccharide	688:701	arg1	composition					703:713	monosaccharide composition	688:713	monosaccharide composition	688:713	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	3	59	theme	psoriasis	593:601	arg1	vulgaris					603:610	psoriasis vulgaris	593:610	psoriasis vulgaris	593:610	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	18	60	theme	psoriasis	2303:2311	arg1	treatment					2290:2298	the treatment	2286:2298	the treatment of psoriasis	2286:2311	This study provides new insight into the medical applications of EPS and the treatment of psoriasis.					
33376326	18	60	theme	psoriasis	2303:2311	arg1	applications					2262:2273	the medical applications	2250:2273	the medical applications of EPS	2250:2280	This study provides new insight into the medical applications of EPS and the treatment of psoriasis.					
33376326	2	61	theme	stronger	284:291	arg1	activity					305:312	stronger emulsifying activity	284:312	stronger emulsifying activity	284:312	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33376326	7	62	theme	size	1058:1061	arg1	distribution					1063:1074	The size distribution	1054:1074	The size distribution	1054:1074	The size distribution, microstructure, stability and in vitro drug release of ECPN were studied.					
33376326	1	63	theme	PURPOSE	149:155	arg1	EPS					182:184	EPS	182:184	EPS	182:184	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	1	63	theme	PURPOSE	149:155	arg1	exopolysaccharides					162:179	PURPOSE Many exopolysaccharides	149:179	PURPOSE Many exopolysaccharides (EPS)	149:185	PURPOSE Many exopolysaccharides (EPS) have significant emulsifying activity.					
33376326	17	64	theme	vulgaris	2203:2210	arg1	treatment					2180:2188	the treatment	2176:2188	the treatment of psoriasis vulgaris	2176:2210	ECPN also showed potential for use in the treatment of psoriasis vulgaris.					
33376326	12	65	theme	emulsification	1794:1807	arg1	mechanism					1809:1817	a Pickering emulsification mechanism	1782:1817	a Pickering emulsification mechanism	1782:1817	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	18	66	theme	new	2233:2235	arg1	insight					2237:2243	new insight	2233:2243	new insight into the medical applications of EPS and the treatment of psoriasis	2233:2311	This study provides new insight into the medical applications of EPS and the treatment of psoriasis.					
33376326	18	67	theme	medical	2254:2260	arg1	applications					2262:2273	the medical applications	2250:2273	the medical applications of EPS	2250:2280	This study provides new insight into the medical applications of EPS and the treatment of psoriasis.					
33376326	5	68	theme	liquid	808:813	arg1	chromatography					815:828	high-performance liquid chromatography	791:828	high-performance liquid chromatography	791:828	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	9	69	theme	Bacillus	1335:1342	arg1	strain					1355:1360	a Bacillus halodurans strain	1333:1360	a Bacillus halodurans strain	1333:1360	RESULTS A phylogenetic tree revealed that FYS was a Bacillus halodurans strain.					
33376326	9	69	theme	Bacillus	1335:1342	arg1	FYS					1325:1327	FYS	1325:1327	FYS	1325:1327	RESULTS A phylogenetic tree revealed that FYS was a Bacillus halodurans strain.					
33376326	8	70	from	effect	1167:1172	arg1	vulgaris					1195:1202	psoriasis vulgaris	1185:1202	psoriasis vulgaris	1185:1202	The therapeutic effect of ECPN on psoriasis vulgaris was explored by animal experiments and characterizing histomorphology in vivo.					
33376326	6	71	theme	surface	1038:1044	arg1	method					1046:1051	the response surface method	1025:1051	the response surface method	1025:1051	EPS nanoparticles were prepared by adjusting the pH, and the emulsifying activity was studied at different pH. ECPN were prepared by ultrasound and optimized by the response surface method.					
33376326	15	72	theme	Pickering	2058:2066	arg1	mechanism					2083:2091	the potential Pickering emulsification mechanism	2044:2091	the potential Pickering emulsification mechanism	2044:2091	CONCLUSION EPS is highly possible to have the potential Pickering emulsification mechanism.					
33376326	5	73	theme	permeation	764:773	arg1	chromatography					775:788	gel permeation chromatography	760:788	gel permeation chromatography	760:788	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	13	74	contain	have	1838:1841	arg2	stability					1848:1856	high stability	1843:1856	high stability	1843:1856	The prepared ECPN have high stability with a sustained-release effect.					
33376326	13	74	contain	have	1838:1841	arg1	ECPN					1833:1836	The prepared ECPN	1820:1836	The prepared ECPN	1820:1836	The prepared ECPN have high stability with a sustained-release effect.					
33376326	18	75	theme	EPS	2278:2280	arg1	treatment					2290:2298	the treatment	2286:2298	the treatment of psoriasis	2286:2311	This study provides new insight into the medical applications of EPS and the treatment of psoriasis.					
33376326	18	75	theme	EPS	2278:2280	arg1	applications					2262:2273	the medical applications	2250:2273	the medical applications of EPS	2250:2280	This study provides new insight into the medical applications of EPS and the treatment of psoriasis.					
33376326	16	76	theme	nanoemulsion	2115:2126	arg1	stability					2098:2106	The stability	2094:2106	The stability of the nanoemulsion	2094:2126	The stability of the nanoemulsion was high.					
33376326	16	76	theme	nanoemulsion	2115:2126	arg1	high					2132:2135	high	2132:2135	high	2132:2135	The stability of the nanoemulsion was high.					
33376326	12	77	theme	droplets	1742:1749	arg1	surface					1727:1733	the surface	1723:1733	the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism	1723:1817	Transmission electron microscopy revealed that EPS nanoparticles adhered to the surface of oil droplets to stabilize the emulsions via a Pickering emulsification mechanism.					
33376326	3	78	theme	novel	421:425	arg1	ECPN					459:462	ECPN	459:462	ECPN	459:462	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	3	78	theme	novel	421:425	arg1	nanoemulsions					444:456	novel EPS/CT Pickering nanoemulsions	421:456	novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers	421:487	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	14	79	theme	animal	1900:1905	arg1	experiments					1907:1917	animal experiments	1900:1917	animal experiments	1900:1917	Finally, animal experiments showed that ECPN effectively shortened the treatment course of psoriasis vulgaris.					
33376326	0	80	theme	Nanoemulsions	45:57	arg1	Effect					12:17	Therapeutic Effect	0:17	Therapeutic Effect of Calcipotriol Pickering Nanoemulsions Prepared by Exopolysaccharides Produced by Bacillus halotolerans	0:122	Therapeutic Effect of Calcipotriol Pickering Nanoemulsions Prepared by Exopolysaccharides Produced by Bacillus halotolerans FYS Strain on Psoriasis.					
33376326	4	81	theme	marine	633:638	arg1	FYS					650:652	marine bacterium FYS	633:652	marine bacterium FYS	633:652	METHODS A strain of marine bacterium FYS was identified.					
33376326	5	82	theme	electron	843:850	arg1	microscopy					852:861	scanning electron microscopy	834:861	scanning electron microscopy	834:861	Molecular weight, monosaccharide composition and microstructure of EPS were determined by gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy.					
33376326	3	83	theme	Pickering	434:442	arg1	ECPN					459:462	ECPN	459:462	ECPN	459:462	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	3	83	theme	Pickering	434:442	arg1	nanoemulsions					444:456	novel EPS/CT Pickering nanoemulsions	421:456	novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers	421:487	We prepared novel EPS/CT Pickering nanoemulsions (ECPN) with EPS as emulsifiers and assessed their ability to ameliorate the poor permeability of calcipotriol (CT) in skin affected by psoriasis vulgaris.					
33376326	8	84	theme	ECPN	1177:1180	arg1	effect					1167:1172	The therapeutic effect	1151:1172	The therapeutic effect of ECPN on psoriasis vulgaris	1151:1202	The therapeutic effect of ECPN on psoriasis vulgaris was explored by animal experiments and characterizing histomorphology in vivo.					
33376326	9	85	dep	RESULTS	1283:1289	arg1	revealed					1311:1318	revealed	1311:1318	revealed that FYS was a Bacillus halodurans strain	1311:1360	RESULTS A phylogenetic tree revealed that FYS was a Bacillus halodurans strain.					
33376326	2	86	theme	Pickering	388:396	arg1	emulsions					398:406	Pickering emulsions	388:406	Pickering emulsions	388:406	Some EPS produced by the marine bacterial strain FYS have stronger emulsifying activity in the form of nanoparticles, suggesting that they could potentially form Pickering emulsions.					
33415933	9	0	dep	mutans	1104:1109	arg1	colonies					1167:1174	colonies	1167:1174	colonies	1167:1174	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.					
33415933	3	1	theme	%	544:544	arg1	CNPs					559:562	0%, 1%, 5%, and 10% CNPs	539:562	0%, 1%, 5%, and 10% CNPs	539:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	12	2	theme	5	1635:1635	arg1	%					1636:1636	%	1636:1636	%	1636:1636	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	4	3	theme	Wistar	597:602	arg1	rats					604:607	The Wistar rats	593:607	The Wistar rats	593:607	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	10	4	theme	orthodontic	1316:1326	arg1	primer					1328:1333	orthodontic primer	1316:1333	orthodontic primer	1316:1333	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	2	5	theme	antibacterial	317:329	arg1	effects					331:337	the antibacterial effects	313:337	the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria	313:456	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	10	6	theme	CNPs	1308:1311	arg1	CNPs					1308:1311	CNPs	1308:1311	CNPs	1308:1311	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	10	6	theme	CNPs	1308:1311	arg1	%					1293:1293	10%	1291:1293	10% (p<0.001) of CNPs	1291:1311	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	10	6	theme	CNPs	1308:1311	arg1	%					1261:1261	1%	1260:1261	1% (p=0.005)	1260:1271	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	10	6	theme	CNPs	1308:1311	arg1	%					1275:1275	5%	1274:1275	5% (p<0.001)	1274:1285	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	10	6	theme	CNPs	1308:1311	arg1	p<0.001					1278:1284	p<0.001	1278:1284	p<0.001	1278:1284	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	10	6	theme	CNPs	1308:1311	arg1	p<0.001					1296:1302	p<0.001	1296:1302	p<0.001	1296:1302	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	10	6	theme	CNPs	1308:1311	arg1	p=0.005					1264:1270	p=0.005	1264:1270	p=0.005	1264:1270	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	0	7	theme	Antibacterial	0:12	arg1	Effects					14:20	Antibacterial Effects	0:20	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.	0:147	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	1	8	theme	orthodontic	200:210	arg1	brackets					212:219	orthodontic brackets	200:219	orthodontic brackets	200:219	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	12	9	theme	colony	1614:1619	arg1	lower					1676:1680	lower	1676:1680	lower	1676:1680	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	12	9	theme	colony	1614:1619	arg1	counts					1621:1626	the mean colony counts	1605:1626	the mean colony counts in the 5% and 10% CNP groups	1605:1655	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	4	10	theme	control	657:663	arg1	%					677:677	1%	676:677	1%	676:677	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	10	theme	control	657:663	arg1	groups					641:646	four groups	636:646	four groups (n=7) of control (0% CNPs)	636:673	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	10	theme	control	657:663	arg1	CNPs					691:694	10% CNPs	687:694	10% CNPs	687:694	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	10	theme	control	657:663	arg1	CNPs					669:672	0% CNPs	666:672	0% CNPs	666:672	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	10	theme	control	657:663	arg1	n=7					649:651	n=7	649:651	n=7	649:651	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	10	theme	control	657:663	arg1	%					681:681	5%	680:681	5%	680:681	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	10	theme	control	657:663	arg1	control					657:663	control	657:663	control (0% CNPs)	657:673	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	11	11	theme	10	1501:1502	arg1	%					1503:1503	%	1503:1503	%	1503:1503	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	2	12	theme	primer	354:359	arg1	effects					331:337	the antibacterial effects	313:337	the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria	313:456	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	15	13	theme	bacteria	2121:2128	arg1	count					2101:2105	the colony count	2090:2105	the colony count of cariogenic bacteria in rats	2090:2136	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	5	14	theme	cariogenic	746:755	arg1	bacteria					757:764	cariogenic bacteria	746:764	cariogenic bacteria	746:764	The oral cavities of the rats were infected with cariogenic bacteria.					
33415933	7	15	theme	adhesive	954:961	arg1	mm					970:971	2 × 2 mm	964:971	2 × 2 mm	964:971	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	7	15	theme	adhesive	954:961	arg1	orthodontic					942:952	Transbond XT orthodontic adhesive	929:961	Transbond XT orthodontic adhesive (2 × 2 mm)	929:972	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	1	16	theme	common	240:245	arg1	complication					247:258	a common complication	238:258	a common complication of fixed orth-odontic treatment	238:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	1	16	theme	common	240:245	arg1	accumulation					180:191	INTRODUCTION Microbial biofilm accumulation	149:191	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite	149:233	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	6	17	theme	different	827:835	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of CNPs	827:858	After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds.					
33415933	10	18	theme	mutans	1364:1369	arg1	count					1378:1382	the S. mutans colony count	1357:1382	the S. mutans colony count	1357:1382	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	7	19	theme	XT	939:940	arg1	mm					970:971	2 × 2 mm	964:971	2 × 2 mm	964:971	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	7	19	theme	XT	939:940	arg1	orthodontic					942:952	Transbond XT orthodontic adhesive	929:961	Transbond XT orthodontic adhesive (2 × 2 mm)	929:972	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	6	20	theme	primer	815:820	arg1	drop					799:802	1 drop	797:802	1 drop (10 µL) of primer with different concentrations of CNPs	797:858	After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds.					
33415933	6	20	theme	primer	815:820	arg1	µL					808:809	10 µL	805:809	10 µL	805:809	After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds.					
33415933	12	21	theme	control	1699:1705	arg1	p<0.05					1714:1719	p<0.05	1714:1719	p<0.05	1714:1719	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	12	21	theme	control	1699:1705	arg1	group					1707:1711	the control group	1695:1711	the control group (p<0.05)	1695:1720	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	9	22	theme	mutans	1104:1109	arg1	number					1080:1085	The number	1076:1085	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats	1076:1196	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.					
33415933	13	23	theme	control	1846:1852	arg1	p<0.05					1861:1866	p<0.05	1861:1866	p<0.05	1861:1866	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	13	23	theme	control	1846:1852	arg1	group					1854:1858	the control group	1842:1858	the control group (p<0.05)	1842:1867	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	11	24	theme	colony	1464:1469	arg1	lower					1544:1548	lower	1544:1548	lower	1544:1548	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	11	24	theme	colony	1464:1469	arg1	counts					1471:1476	the mean S. sanguinis colony counts	1442:1476	the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups	1442:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	1	25	theme	Microbial	162:170	arg1	complication					247:258	a common complication	238:258	a common complication of fixed orth-odontic treatment	238:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	1	25	theme	Microbial	162:170	arg1	accumulation					180:191	INTRODUCTION Microbial biofilm accumulation	149:191	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite	149:233	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	0	26	theme	Multispecies	89:100	arg1	Biofilm					102:108	the Multispecies Biofilm	85:108	the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model	85:146	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	6	27	theme	central	881:887	arg1	incisor					889:895	their central incisor	875:895	their central incisor	875:895	After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds.					
33415933	4	28	theme	%	689:689	arg1	groups					641:646	four groups	636:646	four groups (n=7) of control (0% CNPs)	636:673	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	28	theme	%	689:689	arg1	CNPs					691:694	10% CNPs	687:694	10% CNPs	687:694	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	28	theme	%	689:689	arg1	control					657:663	control	657:663	control (0% CNPs)	657:673	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	11	29	theme	5	1485:1485	arg1	%					1486:1486	%	1486:1486	%	1486:1486	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	15	30	theme	%	2037:2037	arg1	CNPs					2039:2042	5% CNPs	2036:2042	5% CNPs	2036:2042	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	3	31	contain	containing	528:537	arg2	CNPs					559:562	0%, 1%, 5%, and 10% CNPs	539:562	0%, 1%, 5%, and 10% CNPs	539:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	3	31	contain	containing	528:537	arg1	primer					521:526	MATERIALS AND METHODS Transbond XT orthodontic primer	474:526	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs	474:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	2	32	theme	bacteria	449:456	arg1	biofilm					427:433	the multispecies biofilm	410:433	the multispecies biofilm of cariogenic bacteria	410:456	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	0	33	theme	Bacteria	124:131	arg1	Biofilm					102:108	the Multispecies Biofilm	85:108	the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model	85:146	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	15	34	dep	CONCLUSIONS	2008:2018	arg1	decreased					2080:2088	decreased	2080:2088	decreased the colony count of cariogenic bacteria in rats	2080:2136	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	11	35	theme	control	1567:1573	arg1	group					1575:1579	the control group	1563:1579	the control group	1563:1579	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	5	36	theme	rats	722:725	arg1	cavities					706:713	The oral cavities	697:713	The oral cavities of the rats	697:725	The oral cavities of the rats were infected with cariogenic bacteria.					
33415933	13	37	theme	10	1791:1792	arg1	%					1793:1793	%	1793:1793	%	1793:1793	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	3	38	theme	orthodontic	509:519	arg1	primer					521:526	MATERIALS AND METHODS Transbond XT orthodontic primer	474:526	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs	474:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	13	39	theme	CNP	1795:1797	arg1	group					1799:1803	the 10% CNP group	1787:1803	the 10% CNP group	1787:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	3	40	theme	Transbond	496:504	arg1	primer					521:526	MATERIALS AND METHODS Transbond XT orthodontic primer	474:526	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs	474:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	10	41	theme	control	1414:1420	arg1	group					1422:1426	the control group	1410:1426	the control group	1410:1426	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	0	42	theme	Primer	37:42	arg1	Nanoparticles					63:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	13	43	theme	colony	1771:1776	arg1	lower					1823:1827	lower	1823:1827	lower	1823:1827	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	13	43	theme	colony	1771:1776	arg1	count					1778:1782	the mean L. acidophilus colony count	1747:1782	the mean L. acidophilus colony count in the 10% CNP group	1747:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	5	44	theme	oral	701:704	arg1	cavities					706:713	The oral cavities	697:713	The oral cavities of the rats	697:725	The oral cavities of the rats were infected with cariogenic bacteria.					
33415933	14	45	with	rats	1881:1884	arg1	adhesive					1898:1905	adhesive	1898:1905	adhesive	1898:1905	At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).					
33415933	3	46	theme	%	540:540	arg1	CNPs					559:562	0%, 1%, 5%, and 10% CNPs	539:562	0%, 1%, 5%, and 10% CNPs	539:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	0	47	theme	Chitosan	54:61	arg1	Nanoparticles					63:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	15	48	theme	cariogenic	2110:2119	arg1	bacteria					2121:2128	cariogenic bacteria	2110:2128	cariogenic bacteria	2110:2128	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	11	49	theme	mean	1446:1449	arg1	lower					1544:1548	lower	1544:1548	lower	1544:1548	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	11	49	theme	mean	1446:1449	arg1	counts					1471:1476	the mean S. sanguinis colony counts	1442:1476	the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups	1442:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	2	50	theme	multispecies	414:425	arg1	biofilm					427:433	the multispecies biofilm	410:433	the multispecies biofilm of cariogenic bacteria	410:456	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	1	51	theme	fixed	263:267	arg1	treatment					282:290	fixed orth-odontic treatment	263:290	fixed orth-odontic treatment	263:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	12	52	theme	10	1642:1643	arg1	%					1644:1644	%	1644:1644	%	1644:1644	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	8	53	theme	primer	1025:1030	arg1	drop					1009:1012	Another drop	1001:1012	Another drop (10 µL) of primer	1001:1030	Another drop (10 µL) of primer was applied and light-cured for 40 seconds.					
33415933	8	53	theme	primer	1025:1030	arg1	µL					1018:1019	10 µL	1015:1019	10 µL	1015:1019	Another drop (10 µL) of primer was applied and light-cured for 40 seconds.					
33415933	14	54	theme	higher	1930:1935	arg1	count					1937:1941	a significantly higher count	1914:1941	a significantly higher count of all three bacteria	1914:1963	At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).					
33415933	1	55	theme	treatment	282:290	arg1	complication					247:258	a common complication	238:258	a common complication of fixed orth-odontic treatment	238:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	1	55	theme	treatment	282:290	arg1	accumulation					180:191	INTRODUCTION Microbial biofilm accumulation	149:191	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite	149:233	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	12	56	theme	CNP	1646:1648	arg1	groups					1650:1655	the 5% and 10% CNP groups	1631:1655	the 5% and 10% CNP groups	1631:1655	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	11	57	dep	S.	1451:1452	arg1	sanguinis					1454:1462	sanguinis	1454:1462	sanguinis	1454:1462	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	9	58	from	number	1080:1085	arg1	saliva					1183:1188	the saliva	1179:1188	the saliva of rats	1179:1196	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.					
33415933	3	59	theme	%	548:548	arg1	CNPs					559:562	0%, 1%, 5%, and 10% CNPs	539:562	0%, 1%, 5%, and 10% CNPs	539:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	12	60	theme	%	1636:1636	arg1	groups					1650:1655	the 5% and 10% CNP groups	1631:1655	the 5% and 10% CNP groups	1631:1655	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	10	61	theme	S.	1361:1362	arg1	count					1378:1382	the S. mutans colony count	1357:1382	the S. mutans colony count	1357:1382	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	7	62	theme	×	966:966	arg1	mm					970:971	2 × 2 mm	964:971	2 × 2 mm	964:971	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	7	62	theme	×	966:966	arg1	orthodontic					942:952	Transbond XT orthodontic adhesive	929:961	Transbond XT orthodontic adhesive (2 × 2 mm)	929:972	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	12	63	theme	mean	1609:1612	arg1	lower					1676:1680	lower	1676:1680	lower	1676:1680	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	12	63	theme	mean	1609:1612	arg1	counts					1621:1626	the mean colony counts	1605:1626	the mean colony counts in the 5% and 10% CNP groups	1605:1655	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	6	64	with	primer	815:820	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of CNPs	827:858	After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds.					
33415933	11	65	theme	%	1503:1503	arg1	groups					1518:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	0	66	theme	Rat	138:140	arg1	Model					142:146	a Rat Model	136:146	a Rat Model	136:146	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	2	67	theme	orthodontic	342:352	arg1	primer					354:359	orthodontic primer	342:359	orthodontic primer containing chitosan nanoparticles (CNPs)	342:400	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	6	68	theme	CNPs	855:858	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of CNPs	827:858	After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds.					
33415933	0	69	from	Biofilm	102:108	arg1	Model					142:146	a Rat Model	136:146	a Rat Model	136:146	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	10	70	theme	colony	1371:1376	arg1	count					1378:1382	the S. mutans colony count	1357:1382	the S. mutans colony count	1357:1382	RESULTS Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group.					
33415933	11	71	theme	CNP	1514:1516	arg1	groups					1518:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	7	72	theme	Transbond	929:937	arg1	mm					970:971	2 × 2 mm	964:971	2 × 2 mm	964:971	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	7	72	theme	Transbond	929:937	arg1	orthodontic					942:952	Transbond XT orthodontic adhesive	929:961	Transbond XT orthodontic adhesive (2 × 2 mm)	929:972	Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer.					
33415933	11	73	from	counts	1471:1476	arg1	groups					1518:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	11	74	dep	%	1486:1486	arg1	p=0.02					1506:1511	p=0.02	1506:1511	p=0.02	1506:1511	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	11	74	dep	%	1486:1486	arg1	p=0.04					1489:1494	p=0.04	1489:1494	p=0.04	1489:1494	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	3	75	theme	%	557:557	arg1	CNPs					559:562	0%, 1%, 5%, and 10% CNPs	539:562	0%, 1%, 5%, and 10% CNPs	539:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	14	76	with	rats	1979:1982	arg1	adhesive					1989:1996	adhesive	1989:1996	adhesive	1989:1996	At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).					
33415933	14	76	with	rats	1979:1982	arg1	p<0.05					1999:2004	p<0.05	1999:2004	p<0.05	1999:2004	At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).					
33415933	1	77	theme	INTRODUCTION	149:160	arg1	complication					247:258	a common complication	238:258	a common complication of fixed orth-odontic treatment	238:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	1	77	theme	INTRODUCTION	149:160	arg1	accumulation					180:191	INTRODUCTION Microbial biofilm accumulation	149:191	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite	149:233	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	9	78	theme	sanguinis	1126:1134	arg1	number					1080:1085	The number	1076:1085	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats	1076:1196	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.					
33415933	1	79	theme	biofilm	172:178	arg1	complication					247:258	a common complication	238:258	a common complication of fixed orth-odontic treatment	238:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	1	79	theme	biofilm	172:178	arg1	accumulation					180:191	INTRODUCTION Microbial biofilm accumulation	149:191	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite	149:233	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
33415933	4	80	theme	%	667:667	arg1	CNPs					669:672	0% CNPs	666:672	0% CNPs	666:672	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	4	80	theme	%	667:667	arg1	control					657:663	control	657:663	control (0% CNPs)	657:673	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	0	81	theme	Cariogenic	113:122	arg1	Bacteria					124:131	Cariogenic Bacteria	113:131	Cariogenic Bacteria	113:131	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	15	82	theme	orthodontic	2047:2057	arg1	primer					2059:2064	orthodontic primer	2047:2064	orthodontic primer	2047:2064	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	13	83	from	count	1778:1782	arg1	group					1799:1803	the 10% CNP group	1787:1803	the 10% CNP group	1787:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	4	84	theme	10	687:688	arg1	%					689:689	%	689:689	%	689:689	The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs.					
33415933	11	85	theme	%	1486:1486	arg1	groups					1518:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	the 5% (p=0.04) and 10% (p=0.02) CNP groups	1481:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	9	86	theme	acidophilus	1155:1165	arg1	number					1080:1085	The number	1076:1085	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats	1076:1196	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.					
33415933	15	87	theme	CNPs	2039:2042	arg1	addition					2024:2031	The addition	2020:2031	The addition of 5% CNPs to orthodontic primer	2020:2064	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	2	88	theme	а	461:461	arg1	model					467:471	а rat model	461:471	а rat model	461:471	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	3	89	theme	METHODS	488:494	arg1	primer					521:526	MATERIALS AND METHODS Transbond XT orthodontic primer	474:526	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs	474:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	15	90	theme	5	2036:2036	arg1	%					2037:2037	%	2037:2037	%	2037:2037	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	15	91	from	count	2101:2105	arg1	rats					2133:2136	rats	2133:2136	rats	2133:2136	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	3	92	theme	MATERIALS	474:482	arg1	primer					521:526	MATERIALS AND METHODS Transbond XT orthodontic primer	474:526	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs	474:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	0	93	theme	Orthodontic	25:35	arg1	Nanoparticles					63:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	13	94	theme	%	1793:1793	arg1	group					1799:1803	the 10% CNP group	1787:1803	the 10% CNP group	1787:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	2	95	theme	rat	463:465	arg1	model					467:471	а rat model	461:471	а rat model	461:471	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	0	96	theme	Harboring	44:52	arg1	Nanoparticles					63:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Orthodontic Primer Harboring Chitosan Nanoparticles	25:75	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	9	97	theme	rats	1193:1196	arg1	saliva					1183:1188	the saliva	1179:1188	the saliva of rats	1179:1196	The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.					
33415933	12	98	from	counts	1621:1626	arg1	groups					1650:1655	the 5% and 10% CNP groups	1631:1655	the 5% and 10% CNP groups	1631:1655	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	13	99	theme	L.	1756:1757	arg1	lower					1823:1827	lower	1823:1827	lower	1823:1827	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	13	99	theme	L.	1756:1757	arg1	count					1778:1782	the mean L. acidophilus colony count	1747:1782	the mean L. acidophilus colony count in the 10% CNP group	1747:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	3	100	theme	XT	506:507	arg1	primer					521:526	MATERIALS AND METHODS Transbond XT orthodontic primer	474:526	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs	474:562	MATERIALS AND METHODS Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared.					
33415933	0	101	theme	Nanoparticles	63:75	arg1	Effects					14:20	Antibacterial Effects	0:20	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.	0:147	Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model.					
33415933	11	102	theme	S.	1451:1452	arg1	lower					1544:1548	lower	1544:1548	lower	1544:1548	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	11	102	theme	S.	1451:1452	arg1	counts					1471:1476	the mean S. sanguinis colony counts	1442:1476	the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups	1442:1523	At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group.					
33415933	14	103	theme	bacteria	1956:1963	arg1	count					1937:1941	a significantly higher count	1914:1941	a significantly higher count of all three bacteria	1914:1963	At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).					
33415933	2	104	contain	containing	361:370	arg2	CNPs					396:399	CNPs	396:399	CNPs	396:399	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	2	104	contain	containing	361:370	arg1	primer					354:359	orthodontic primer	342:359	orthodontic primer containing chitosan nanoparticles (CNPs)	342:400	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	2	104	contain	containing	361:370	arg2	nanoparticles					381:393	chitosan nanoparticles	372:393	chitosan nanoparticles (CNPs)	372:400	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	2	105	theme	chitosan	372:379	arg1	CNPs					396:399	CNPs	396:399	CNPs	396:399	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	2	105	theme	chitosan	372:379	arg1	nanoparticles					381:393	chitosan nanoparticles	372:393	chitosan nanoparticles (CNPs)	372:400	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	15	106	theme	colony	2094:2099	arg1	count					2101:2105	the colony count	2090:2105	the colony count of cariogenic bacteria in rats	2090:2136	CONCLUSIONS The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.					
33415933	13	107	theme	mean	1751:1754	arg1	lower					1823:1827	lower	1823:1827	lower	1823:1827	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	13	107	theme	mean	1751:1754	arg1	count					1778:1782	the mean L. acidophilus colony count	1747:1782	the mean L. acidophilus colony count in the 10% CNP group	1747:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	12	108	theme	%	1644:1644	arg1	groups					1650:1655	the 5% and 10% CNP groups	1631:1655	the 5% and 10% CNP groups	1631:1655	Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05).					
33415933	2	109	theme	cariogenic	438:447	arg1	bacteria					449:456	cariogenic bacteria	438:456	cariogenic bacteria	438:456	This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.					
33415933	13	110	theme	acidophilus	1759:1769	arg1	lower					1823:1827	lower	1823:1827	lower	1823:1827	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	13	110	theme	acidophilus	1759:1769	arg1	count					1778:1782	the mean L. acidophilus colony count	1747:1782	the mean L. acidophilus colony count in the 10% CNP group	1747:1803	At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05).					
33415933	1	111	theme	orth-odontic	269:280	arg1	treatment					282:290	fixed orth-odontic treatment	263:290	fixed orth-odontic treatment	263:290	INTRODUCTION Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment.					
34779470	7	0	theme	adsorption	1174:1183	arg1	capacity					1185:1192	a high adsorption capacity	1167:1192	a high adsorption capacity (388.6 mg g-1)	1167:1207	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	0	theme	adsorption	1174:1183	arg1	g-1					1204:1206	388.6 mg g-1	1195:1206	388.6 mg g-1	1195:1206	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	1	theme	albumin	1111:1117	arg1	properties					1084:1093	the adsorption and release properties	1057:1093	properties	1084:1093	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	2	theme	mg	1201:1202	arg1	capacity					1185:1192	a high adsorption capacity	1167:1192	a high adsorption capacity (388.6 mg g-1)	1167:1207	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	2	theme	mg	1201:1202	arg1	g-1					1204:1206	388.6 mg g-1	1195:1206	388.6 mg g-1	1195:1206	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	5	3	from	burning	803:809	arg1	air					814:816	air	814:816	air	814:816	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	7	4	theme	adsorption	1061:1070	arg1	properties					1084:1093	the adsorption and release properties	1057:1093	properties	1084:1093	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	3	5	theme	hierarchical	491:502	arg1	structure					509:517	a hierarchical pore structure	489:517	a hierarchical pore structure	489:517	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	6	6	theme	porous	988:993	arg1	structure					995:1003	a hierarchically porous structure	971:1003	a hierarchically porous structure	971:1003	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	5	7	theme	cellulose	661:669	arg1	monoliths					671:679	The cellulose monoliths	657:679	The cellulose monoliths	657:679	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	6	8	theme	porous	880:885	arg1	structure					887:895	the hierarchically porous structure	861:895	the hierarchically porous structure of the cellulose monolith template	861:930	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	1	9	theme	bone	166:169	arg1	defects					171:177	bone defects	166:177	bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity	166:289	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	7	10	theme	high	1169:1172	arg1	capacity					1185:1192	a high adsorption capacity	1167:1192	a high adsorption capacity (388.6 mg g-1)	1167:1207	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	10	theme	high	1169:1172	arg1	g-1					1204:1206	388.6 mg g-1	1195:1206	388.6 mg g-1	1195:1206	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	4	11	theme	induced	624:630	arg1	method					649:654	a thermally induced phase separation method	612:654	a thermally induced phase separation method	612:654	The cellulose monolith template was prepared from cellulose acetate using a thermally induced phase separation method.					
34779470	7	12	theme	serum	1105:1109	arg1	BSA					1120:1122	BSA	1120:1122	BSA	1120:1122	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	12	theme	serum	1105:1109	arg1	albumin					1111:1117	bovine serum albumin	1098:1117	bovine serum albumin (BSA)	1098:1123	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	5	13	theme	cellulose	832:840	arg1	monolith					842:849	the cellulose monolith	828:849	the cellulose monolith	828:849	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	5	14	theme	cellulose_HA	729:740	arg1	monolith					752:759	a cellulose_HA composite monolith	727:759	a cellulose_HA composite monolith	727:759	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	2	15	from	structure	338:346	arg1	materials					354:362	HA materials	351:362	HA materials	351:362	However, it is difficult to maintain a porous structure in HA materials because of processing difficulties.					
34779470	1	16	theme	defects	171:177	arg1	treatment					153:161	the clinical treatment	140:161	the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity	140:289	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	5	17	theme	composite	742:750	arg1	monolith					752:759	a cellulose_HA composite monolith	727:759	a cellulose_HA composite monolith	727:759	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	8	18	theme	HA	1270:1271	arg1	monoliths					1273:1281	HA monoliths	1270:1281	HA monoliths	1270:1281	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	3	19	theme	high	523:526	arg1	porosity					528:535	high porosity	523:535	high porosity	523:535	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	8	20	contain	have	1283:1286	arg2	applications					1298:1309	potential applications	1288:1309	potential applications	1288:1309	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	8	20	contain	have	1283:1286	arg1	monoliths					1273:1281	HA monoliths	1270:1281	HA monoliths	1270:1281	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	6	21	theme	obtained	937:944	arg1	monolith					949:956	the obtained HA monolith	933:956	the obtained HA monolith	933:956	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	3	22	with	monolith	475:482	arg1	porosity					528:535	high porosity	523:535	high porosity	523:535	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	3	22	with	monolith	475:482	arg1	structure					509:517	a hierarchical pore structure	489:517	a hierarchical pore structure	489:517	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	3	23	theme	hard	417:420	arg1	method					431:436	a hard template method	415:436	a hard template method	415:436	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	0	24	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of a three-dimensional hydroxyapatite	0:53	Facile synthesis of a three-dimensional hydroxyapatite monolith for protein adsorption.					
34779470	4	25	theme	cellulose	542:550	arg1	template					561:568	The cellulose monolith template	538:568	The cellulose monolith template	538:568	The cellulose monolith template was prepared from cellulose acetate using a thermally induced phase separation method.					
34779470	7	26	theme	release	1076:1082	arg1	properties					1084:1093	the adsorption and release properties	1057:1093	properties	1084:1093	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	3	27	theme	template	422:429	arg1	method					431:436	a hard template method	415:436	a hard template method	415:436	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	8	28	theme	protein	1327:1333	arg1	purification					1335:1346	protein purification	1327:1346	protein purification	1327:1346	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	1	29	theme	excellent	192:200	arg1	biocompatibility					238:253	biocompatibility	238:253	biocompatibility	238:253	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	1	29	theme	excellent	192:200	arg1	properties					218:227	its excellent physicochemical properties	188:227	its excellent physicochemical properties	188:227	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	1	29	theme	excellent	192:200	arg1	bioactivity					256:266	bioactivity	256:266	bioactivity	256:266	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	1	29	theme	excellent	192:200	arg1	osteoconductivity					273:289	osteoconductivity	273:289	osteoconductivity	273:289	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	6	30	theme	HA	946:947	arg1	monolith					949:956	the obtained HA monolith	933:956	the obtained HA monolith	933:956	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	5	31	theme	HA	709:710	arg1	slurry					712:717	the HA slurry	705:717	the HA slurry	705:717	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	2	32	theme	HA	351:352	arg1	materials					354:362	HA materials	351:362	HA materials	351:362	However, it is difficult to maintain a porous structure in HA materials because of processing difficulties.					
34779470	1	33	theme	physicochemical	202:216	arg1	biocompatibility					238:253	biocompatibility	238:253	biocompatibility	238:253	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	1	33	theme	physicochemical	202:216	arg1	properties					218:227	its excellent physicochemical properties	188:227	its excellent physicochemical properties	188:227	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	1	33	theme	physicochemical	202:216	arg1	bioactivity					256:266	bioactivity	256:266	bioactivity	256:266	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	1	33	theme	physicochemical	202:216	arg1	osteoconductivity					273:289	osteoconductivity	273:289	osteoconductivity	273:289	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	0	34	theme	hydroxyapatite	40:53	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of a three-dimensional hydroxyapatite	0:53	Facile synthesis of a three-dimensional hydroxyapatite monolith for protein adsorption.					
34779470	4	35	theme	monolith	552:559	arg1	template					561:568	The cellulose monolith template	538:568	The cellulose monolith template	538:568	The cellulose monolith template was prepared from cellulose acetate using a thermally induced phase separation method.					
34779470	7	36	theme	HA	1151:1152	arg1	monolith					1154:1161	the HA monolith	1147:1161	the HA monolith	1147:1161	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	2	37	theme	processing	375:384	arg1	difficulties					386:397	processing difficulties	375:397	processing difficulties	375:397	However, it is difficult to maintain a porous structure in HA materials because of processing difficulties.					
34779470	7	38	theme	BSA-loaded	1240:1249	arg1	monolith					1254:1261	the BSA-loaded HA monolith	1236:1261	the BSA-loaded HA monolith	1236:1261	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	0	39	theme	three-dimensional	22:38	arg1	hydroxyapatite					40:53	a three-dimensional hydroxyapatite	20:53	a three-dimensional hydroxyapatite	20:53	Facile synthesis of a three-dimensional hydroxyapatite monolith for protein adsorption.					
34779470	8	40	theme	purification	1335:1346	arg1	field					1318:1322	the field	1314:1322	the field of protein purification and biomaterials	1314:1363	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	7	41	contain	had	1163:1165	arg1	monolith					1154:1161	the HA monolith	1147:1161	the HA monolith	1147:1161	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	41	contain	had	1163:1165	arg2	release					1223:1229	sustained release	1213:1229	sustained release from the BSA-loaded HA monolith	1213:1261	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	41	contain	had	1163:1165	arg2	capacity					1185:1192	a high adsorption capacity	1167:1192	a high adsorption capacity (388.6 mg g-1)	1167:1207	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	41	contain	had	1163:1165	arg2	g-1					1204:1206	388.6 mg g-1	1195:1206	388.6 mg g-1	1195:1206	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	4	42	theme	separation	638:647	arg1	method					649:654	a thermally induced phase separation method	612:654	a thermally induced phase separation method	612:654	The cellulose monolith template was prepared from cellulose acetate using a thermally induced phase separation method.					
34779470	7	43	theme	388.6	1195:1199	arg1	mg					1201:1202	mg	1201:1202	mg	1201:1202	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	1	44	theme	promising	114:122	arg1	applications					124:135	promising applications	114:135	promising applications	114:135	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	5	45	theme	HA	788:789	arg1	monolith					791:798	an HA monolith	785:798	an HA monolith	785:798	The cellulose monoliths were then immersed into the HA slurry to form a cellulose_HA composite monolith, which was converted to an HA monolith by burning in air to remove the cellulose monolith.					
34779470	6	46	theme	template	923:930	arg1	structure					887:895	the hierarchically porous structure	861:895	the hierarchically porous structure of the cellulose monolith template	861:930	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	4	47	theme	cellulose	588:596	arg1	acetate					598:604	cellulose acetate	588:604	cellulose acetate using a thermally induced phase separation method	588:654	The cellulose monolith template was prepared from cellulose acetate using a thermally induced phase separation method.					
34779470	8	48	theme	biomaterials	1352:1363	arg1	field					1318:1322	the field	1314:1322	the field of protein purification and biomaterials	1314:1363	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	7	49	from	monolith	1254:1261	arg1	capacity					1185:1192	a high adsorption capacity	1167:1192	a high adsorption capacity (388.6 mg g-1)	1167:1207	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	49	from	monolith	1254:1261	arg1	g-1					1204:1206	388.6 mg g-1	1195:1206	388.6 mg g-1	1195:1206	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	49	from	monolith	1254:1261	arg1	release					1223:1229	sustained release	1213:1229	sustained release from the BSA-loaded HA monolith	1213:1261	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	50	theme	bovine	1098:1103	arg1	BSA					1120:1122	BSA	1120:1122	BSA	1120:1122	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	7	50	theme	bovine	1098:1103	arg1	albumin					1111:1117	bovine serum albumin	1098:1117	bovine serum albumin (BSA)	1098:1123	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	6	51	theme	monolith	914:921	arg1	template					923:930	the cellulose monolith template	900:930	the cellulose monolith template	900:930	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	7	52	theme	HA	1023:1024	arg1	monolith					1026:1033	the HA monolith	1019:1033	the HA monolith	1019:1033	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	1	53	theme	clinical	144:151	arg1	treatment					153:161	the clinical treatment	140:161	the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity	140:289	Hydroxyapatite (HA) shows promising applications in the clinical treatment of bone defects owing to its excellent physicochemical properties, such as biocompatibility, bioactivity, and osteoconductivity.					
34779470	2	54	theme	porous	331:336	arg1	structure					338:346	a porous structure	329:346	a porous structure in HA materials	329:362	However, it is difficult to maintain a porous structure in HA materials because of processing difficulties.					
34779470	8	55	theme	potential	1288:1296	arg1	applications					1298:1309	potential applications	1288:1309	potential applications	1288:1309	Thus, HA monoliths have potential applications in the field of protein purification and biomaterials.					
34779470	3	56	theme	HA	472:473	arg1	monolith					475:482	a porous HA monolith	463:482	a porous HA monolith with a hierarchical pore structure and high porosity	463:535	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	3	57	theme	porous	465:470	arg1	monolith					475:482	a porous HA monolith	463:482	a porous HA monolith with a hierarchical pore structure and high porosity	463:535	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	7	58	theme	HA	1251:1252	arg1	monolith					1254:1261	the BSA-loaded HA monolith	1236:1261	the BSA-loaded HA monolith	1236:1261	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34779470	4	59	theme	phase	632:636	arg1	method					649:654	a thermally induced phase separation method	612:654	a thermally induced phase separation method	612:654	The cellulose monolith template was prepared from cellulose acetate using a thermally induced phase separation method.					
34779470	3	60	theme	pore	504:507	arg1	structure					509:517	a hierarchical pore structure	489:517	a hierarchical pore structure	489:517	In this study, a hard template method was developed to prepare a porous HA monolith with a hierarchical pore structure and high porosity.					
34779470	0	61	theme	protein	68:74	arg1	adsorption					76:85	protein adsorption	68:85	protein adsorption	68:85	Facile synthesis of a three-dimensional hydroxyapatite monolith for protein adsorption.					
34779470	6	62	theme	cellulose	904:912	arg1	template					923:930	the cellulose monolith template	900:930	the cellulose monolith template	900:930	Owing to the hierarchically porous structure of the cellulose monolith template, the obtained HA monolith demonstrated a hierarchically porous structure.					
34779470	7	63	theme	sustained	1213:1221	arg1	release					1223:1229	sustained release	1213:1229	sustained release from the BSA-loaded HA monolith	1213:1261	Furthermore, the HA monolith was explored to study the adsorption and release properties of bovine serum albumin (BSA), which indicated that the HA monolith had a high adsorption capacity (388.6 mg g-1) and sustained release from the BSA-loaded HA monolith.					
34284721	4	0	with	regions	996:1002	arg1	QTL					1009:1011	QTL	1009:1011	QTL identified for FT-MIR predicted milk composition traits	1009:1067	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	8	1	theme	QTL	1959:1961	arg1	number					1949:1954	a larger number	1940:1954	a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits	1940:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	2	2	theme	FT-MIR	385:390	arg1	traits					402:407	FT-MIR predicted traits	385:407	FT-MIR predicted traits	385:407	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	4	3	theme	FT-MIR	943:948	arg1	QTL					961:963	significant FT-MIR wavenumber QTL	931:963	significant FT-MIR wavenumber QTL	931:963	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	3	4	theme	physico-chemical	763:778	arg1	properties					780:789	the physico-chemical properties	759:789	the physico-chemical properties of milk	759:797	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	8	5	theme	putative	1967:1974	arg1	genes					1986:1990	putative causative genes	1967:1990	putative causative genes	1967:1990	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	3	6	theme	individual	580:589	arg1	phenotypes					609:618	895 individual FT-MIR wavenumber phenotypes	576:618	895 individual FT-MIR wavenumber phenotypes	576:618	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	8	7	theme	genes	1986:1990	arg1	number					1949:1954	a larger number	1940:1954	a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits	1940:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	6	8	theme	relevant	1501:1508	arg1	loci					1510:1513	relevant loci	1501:1513	relevant loci	1501:1513	For the candidate causative genes implicated in these analyses, we examined the strength of association between relevant loci and each wavenumber across the mid-infrared spectrum.					
34284721	1	9	theme	inexpensive	213:223	arg1	method					225:230	a high-throughput and inexpensive method	191:230	a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples	191:303	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	4	10	theme	genomic	910:916	arg1	regions					918:924	450 1-Mbp genomic regions	900:924	450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL	900:963	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	3	11	theme	wavenumber	598:607	arg1	phenotypes					609:618	895 individual FT-MIR wavenumber phenotypes	576:618	895 individual FT-MIR wavenumber phenotypes	576:618	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	1	12	theme	milk	247:250	arg1	composition					252:262	milk composition	247:262	milk composition	247:262	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	4	13	theme	genomic	988:994	arg1	regions					996:1002	246 1-Mbp genomic regions	978:1002	246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits	978:1067	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	9	14	theme	Examining	2058:2066	arg1	patterns					2068:2075	Examining patterns	2058:2075	Examining patterns of significance across the mid-infrared spectrum for loci of interest	2058:2145	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	8	15	theme	FT-MIR	2021:2026	arg1	traits					2050:2055	FT-MIR predicted composition traits	2021:2055	FT-MIR predicted composition traits	2021:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	5	16	theme	co-localized	1144:1155	arg1	QTL					1187:1189	expression QTL	1176:1189	expression QTL (eQTL)	1176:1196	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	16	theme	co-localized	1144:1155	arg1	eQTL					1192:1195	eQTL	1192:1195	eQTL	1192:1195	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	8	17	theme	wavenumber	1854:1863	arg1	phenotypes					1865:1874	FT-MIR wavenumber phenotypes	1847:1874	FT-MIR wavenumber phenotypes	1847:1874	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	0	18	from	wavenumbers	77:87	arg1	cattle					110:115	mixed-breed dairy cattle	92:115	mixed-breed dairy cattle	92:115	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	8	19	theme	composition	2038:2048	arg1	traits					2050:2055	FT-MIR predicted composition traits	2021:2055	FT-MIR predicted composition traits	2021:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	3	20	theme	direct	653:658	arg1	phenotypes					660:669	these direct phenotypes	647:669	these direct phenotypes	647:669	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	5	21	theme	potential	1331:1339	arg1	role					1341:1344	a potential role	1329:1344	a potential role	1329:1344	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	22	from	mutation	1295:1302	arg1	gene					1315:1318	the ABO gene	1307:1318	the ABO gene	1307:1318	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	23	theme	annotation	1107:1116	arg1	information					1118:1128	gene annotation information	1102:1128	gene annotation information	1102:1128	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	3	24	theme	phenotypes	660:669	arg1	value					638:642	the value	634:642	the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk	634:797	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	9	25	theme	mid-infrared	2104:2115	arg1	spectrum					2117:2124	the mid-infrared spectrum	2100:2124	the mid-infrared spectrum for loci of interest	2100:2145	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	6	26	theme	association	1481:1491	arg1	strength					1469:1476	the strength	1465:1476	the strength of association between relevant loci	1465:1513	For the candidate causative genes implicated in these analyses, we examined the strength of association between relevant loci and each wavenumber across the mid-infrared spectrum.					
34284721	6	26	theme	association	1481:1491	arg1	wavenumber					1524:1533	each wavenumber	1519:1533	each wavenumber across the mid-infrared spectrum	1519:1566	For the candidate causative genes implicated in these analyses, we examined the strength of association between relevant loci and each wavenumber across the mid-infrared spectrum.					
34284721	4	27	theme	milk	1045:1048	arg1	traits					1062:1067	milk composition traits	1045:1067	milk composition traits	1045:1067	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	0	28	theme	mixed-breed	92:102	arg1	cattle					110:115	mixed-breed dairy cattle	92:115	mixed-breed dairy cattle	92:115	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	8	29	theme	composition	1916:1926	arg1	understanding					1894:1906	our understanding	1890:1906	our understanding of milk composition	1890:1926	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	1	30	theme	high-throughput	193:207	arg1	method					225:230	a high-throughput and inexpensive method	191:230	a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples	191:303	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	5	31	theme	co-segregating	1161:1174	arg1	QTL					1187:1189	expression QTL	1176:1189	expression QTL (eQTL)	1176:1196	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	31	theme	co-segregating	1161:1174	arg1	eQTL					1192:1195	eQTL	1192:1195	eQTL	1192:1195	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	7	32	from	roles	1713:1717	arg1	lactation					1722:1730	lactation	1722:1730	lactation	1722:1730	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	7	33	theme	loci	1645:1648	arg1	groups					1615:1620	groups	1615:1620	groups of genomically-distant loci	1615:1648	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	7	33	theme	loci	1645:1648	arg1	loci					1645:1648	genomically-distant loci	1625:1648	genomically-distant loci	1625:1648	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	2	34	theme	few	426:428	arg1	GWAS					430:433	few GWAS	426:433	few GWAS for individual FT-MIR wavenumbers	426:467	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	6	35	theme	causative	1407:1415	arg1	genes					1417:1421	the candidate causative genes	1393:1421	the candidate causative genes implicated in these analyses	1393:1450	For the candidate causative genes implicated in these analyses, we examined the strength of association between relevant loci and each wavenumber across the mid-infrared spectrum.					
34284721	8	36	theme	larger	1942:1947	arg1	number					1949:1954	a larger number	1940:1954	a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits	1940:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	7	37	theme	chemical	1766:1773	arg1	composition					1775:1785	the chemical composition	1762:1785	the chemical composition of milk	1762:1793	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	0	38	theme	Sequence-based	0:13	arg1	association					27:37	Sequence-based genome-wide association	0:37	Sequence-based genome-wide association study of individual milk	0:62	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	5	39	theme	FT-MIR	1237:1242	arg1	phenotypes					1255:1264	FT-MIR wavenumber phenotypes	1237:1264	FT-MIR wavenumber phenotypes	1237:1264	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	9	40	theme	association	2184:2194	arg1	commonalities					2167:2179	commonalities	2167:2179	commonalities	2167:2179	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	4	41	theme	RESULTS	800:806	arg1	GWAS					817:820	RESULTS Separate GWAS	800:820	RESULTS Separate GWAS	800:820	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	5	42	theme	milk	1358:1361	arg1	profiles					1379:1386	milk oligosaccharide profiles	1358:1386	milk oligosaccharide profiles	1358:1386	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	2	43	theme	FT-MIR	450:455	arg1	wavenumbers					457:467	individual FT-MIR wavenumbers	439:467	individual FT-MIR wavenumbers	439:467	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	0	44	theme	association	27:37	arg1	study					39:43	Sequence-based genome-wide association study	0:43	Sequence-based genome-wide association study of individual milk	0:62	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	1	45	theme	Fourier-transform	129:145	arg1	spectroscopy					169:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	7	46	theme	presumed	1742:1749	arg1	impacts					1751:1757	their presumed impacts	1736:1757	their presumed impacts on the chemical composition of milk	1736:1793	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	3	47	theme	causal	697:702	arg1	genes					704:708	candidate causal genes	687:708	candidate causal genes	687:708	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	4	48	theme	wavenumber	866:875	arg1	phenotypes					877:886	895 individual FT-MIR wavenumber phenotypes	844:886	895 individual FT-MIR wavenumber phenotypes	844:886	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	3	49	theme	whole-genome	484:495	arg1	sequence					497:504	imputed whole-genome sequence	476:504	imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle	476:552	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	5	50	theme	mammary	1077:1083	arg1	data					1093:1096	mammary RNA-seq data	1077:1096	mammary RNA-seq data	1077:1096	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	3	51	theme	Zealand	533:539	arg1	cattle					547:552	38,085 mixed-breed New Zealand dairy cattle	510:552	38,085 mixed-breed New Zealand dairy cattle	510:552	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	0	52	theme	milk	59:62	arg1	study					39:43	Sequence-based genome-wide association study	0:43	Sequence-based genome-wide association study of individual milk	0:62	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	7	53	theme	shared	1583:1588	arg1	patterns					1602:1609	shared association patterns	1583:1609	shared association patterns for groups of genomically-distant loci	1583:1648	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	5	54	theme	data	1093:1096	arg1	Use					1070:1072	Use	1070:1072	Use of mammary RNA-seq data and gene annotation information	1070:1128	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	9	55	theme	physico-chemical	2223:2238	arg1	properties					2240:2249	the physico-chemical properties	2219:2249	the physico-chemical properties of milk constituents	2219:2270	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	3	56	theme	mixed-breed	517:527	arg1	Zealand					533:539	38,085 mixed-breed New Zealand	510:539	38,085 mixed-breed New Zealand dairy cattle	510:552	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	1	57	theme	other	268:272	arg1	traits					280:285	other novel traits	268:285	other novel traits	268:285	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	4	58	theme	individual	848:857	arg1	phenotypes					877:886	895 individual FT-MIR wavenumber phenotypes	844:886	895 individual FT-MIR wavenumber phenotypes	844:886	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	3	59	from	GWAS	568:571	arg1	phenotypes					609:618	895 individual FT-MIR wavenumber phenotypes	576:618	895 individual FT-MIR wavenumber phenotypes	576:618	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	9	60	theme	constituents	2259:2270	arg1	properties					2240:2249	the physico-chemical properties	2219:2249	the physico-chemical properties of milk constituents	2219:2270	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	2	61	theme	predicted	392:400	arg1	traits					402:407	FT-MIR predicted traits	385:407	FT-MIR predicted traits	385:407	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	1	62	theme	milk	292:295	arg1	samples					297:303	milk samples	292:303	milk samples	292:303	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	8	63	theme	causative	1976:1984	arg1	genes					1986:1990	putative causative genes	1967:1990	putative causative genes	1967:1990	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	2	64	theme	many	328:331	arg1	GWAS					366:369	GWAS	366:369	GWAS	366:369	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	2	64	theme	many	328:331	arg1	studies					357:363	many genome-wide association studies	328:363	many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits	328:407	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	4	65	theme	significant	931:941	arg1	QTL					961:963	significant FT-MIR wavenumber QTL	931:963	significant FT-MIR wavenumber QTL	931:963	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	3	66	theme	properties	780:789	arg1	understanding					742:754	our understanding	738:754	our understanding of the physico-chemical properties of milk	738:797	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	4	67	theme	1-Mbp	904:908	arg1	regions					918:924	450 1-Mbp genomic regions	900:924	450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL	900:963	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	6	68	theme	candidate	1397:1405	arg1	genes					1417:1421	the candidate causative genes	1393:1421	the candidate causative genes implicated in these analyses	1393:1450	For the candidate causative genes implicated in these analyses, we examined the strength of association between relevant loci and each wavenumber across the mid-infrared spectrum.					
34284721	3	69	theme	FT-MIR	591:596	arg1	phenotypes					609:618	895 individual FT-MIR wavenumber phenotypes	576:618	895 individual FT-MIR wavenumber phenotypes	576:618	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	3	70	theme	milk	794:797	arg1	properties					780:789	the physico-chemical properties	759:789	the physico-chemical properties of milk	759:797	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	8	71	theme	predicted	2028:2036	arg1	traits					2050:2055	FT-MIR predicted composition traits	2021:2055	FT-MIR predicted composition traits	2021:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	7	72	theme	biological	1702:1711	arg1	roles					1713:1717	their biological roles	1696:1717	their biological roles in lactation	1696:1730	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	4	73	theme	1-Mbp	982:986	arg1	regions					996:1002	246 1-Mbp genomic regions	978:1002	246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits	978:1067	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	7	74	from	impacts	1751:1757	arg1	composition					1775:1785	the chemical composition	1762:1785	the chemical composition of milk	1762:1793	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	8	75	theme	FT-MIR	1847:1852	arg1	phenotypes					1865:1874	FT-MIR wavenumber phenotypes	1847:1874	FT-MIR wavenumber phenotypes	1847:1874	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	1	76	theme	mid-infrared	147:158	arg1	spectroscopy					169:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	4	77	theme	wavenumber	950:959	arg1	QTL					961:963	significant FT-MIR wavenumber QTL	931:963	significant FT-MIR wavenumber QTL	931:963	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	9	78	theme	significance	2080:2091	arg1	patterns					2068:2075	Examining patterns	2058:2075	Examining patterns of significance across the mid-infrared spectrum for loci of interest	2058:2145	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	5	79	theme	gene	1102:1105	arg1	information					1118:1128	gene annotation information	1102:1128	gene annotation information	1102:1128	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	80	contain	has	1325:1327	arg1	mutation					1295:1302	a null mutation	1288:1302	a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles	1288:1386	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	80	contain	has	1325:1327	arg2	role					1341:1344	a potential role	1329:1344	a potential role	1329:1344	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	8	81	theme	phenotypes	1865:1874	arg1	utility					1836:1842	the utility	1832:1842	the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition	1832:1926	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	1	82	theme	FT-MIR	161:166	arg1	spectroscopy					169:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	2	83	theme	association	345:355	arg1	GWAS					366:369	GWAS	366:369	GWAS	366:369	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	2	83	theme	association	345:355	arg1	studies					357:363	many genome-wide association studies	328:363	many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits	328:407	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	7	84	theme	loci	1676:1679	arg1	impacts					1751:1757	their presumed impacts	1736:1757	their presumed impacts on the chemical composition of milk	1736:1793	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	7	84	theme	loci	1676:1679	arg1	clusters					1664:1671	clusters	1664:1671	clusters of loci linked through their biological roles in lactation	1664:1730	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	5	85	theme	information	1118:1128	arg1	Use					1070:1072	Use	1070:1072	Use of mammary RNA-seq data and gene annotation information	1070:1128	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	4	86	with	regions	918:924	arg1	QTL					961:963	significant FT-MIR wavenumber QTL	931:963	significant FT-MIR wavenumber QTL	931:963	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	5	87	theme	null	1290:1293	arg1	mutation					1295:1302	a null mutation	1288:1302	a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles	1288:1386	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	88	theme	ABO	1311:1313	arg1	gene					1315:1318	the ABO gene	1307:1318	the ABO gene	1307:1318	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	0	89	theme	dairy	104:108	arg1	cattle					110:115	mixed-breed dairy cattle	92:115	mixed-breed dairy cattle	92:115	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	8	90	dep	genes	1986:1990	arg1	found					2010:2014	found	2010:2014	found from FT-MIR predicted composition traits	2010:2055	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	8	91	theme	milk	1911:1914	arg1	composition					1916:1926	milk composition	1911:1926	milk composition	1911:1926	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	2	92	theme	genome-wide	333:343	arg1	GWAS					366:369	GWAS	366:369	GWAS	366:369	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	2	92	theme	genome-wide	333:343	arg1	studies					357:363	many genome-wide association studies	328:363	many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits	328:407	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	7	93	theme	genomically-distant	1625:1643	arg1	loci					1645:1648	genomically-distant loci	1625:1648	genomically-distant loci	1625:1648	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	9	94	theme	interest	2138:2145	arg1	loci					2130:2133	loci	2130:2133	loci of interest	2130:2145	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34284721	4	95	theme	Separate	808:815	arg1	GWAS					817:820	RESULTS Separate GWAS	800:820	RESULTS Separate GWAS	800:820	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	5	96	theme	expression	1176:1185	arg1	QTL					1187:1189	expression QTL	1176:1189	expression QTL (eQTL)	1176:1196	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	96	theme	expression	1176:1185	arg1	eQTL					1192:1195	eQTL	1192:1195	eQTL	1192:1195	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	7	97	theme	milk	1790:1793	arg1	composition					1775:1785	the chemical composition	1762:1785	the chemical composition of milk	1762:1793	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	3	98	theme	imputed	476:482	arg1	sequence					497:504	imputed whole-genome sequence	476:504	imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle	476:552	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	0	99	theme	genome-wide	15:25	arg1	association					27:37	Sequence-based genome-wide association	0:37	Sequence-based genome-wide association study of individual milk	0:62	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	8	100	dep	CONCLUSIONS	1796:1806	arg1	demonstrates					1819:1830	demonstrates	1819:1830	demonstrates	1819:1830	CONCLUSIONS This study demonstrates the utility of FT-MIR wavenumber phenotypes for improving our understanding of milk composition, presenting a larger number of QTL and putative causative genes and variants than found from FT-MIR predicted composition traits.					
34284721	5	101	theme	wavenumber	1244:1253	arg1	phenotypes					1255:1264	FT-MIR wavenumber phenotypes	1237:1264	FT-MIR wavenumber phenotypes	1237:1264	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	1	102	theme	BACKGROUND	118:127	arg1	spectroscopy					169:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy	118:180	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	3	103	theme	candidate	687:695	arg1	genes					704:708	candidate causal genes	687:708	candidate causal genes	687:708	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	5	104	theme	oligosaccharide	1363:1377	arg1	profiles					1379:1386	milk oligosaccharide profiles	1358:1386	milk oligosaccharide profiles	1358:1386	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	2	105	theme	individual	439:448	arg1	wavenumbers					457:467	individual FT-MIR wavenumbers	439:467	individual FT-MIR wavenumbers	439:467	While there have been many genome-wide association studies (GWAS) conducted on FT-MIR predicted traits, there have been few GWAS for individual FT-MIR wavenumbers.					
34284721	0	106	theme	individual	48:57	arg1	milk					59:62	individual milk	48:62	individual milk	48:62	Sequence-based genome-wide association study of individual milk mid-infrared wavenumbers in mixed-breed dairy cattle.					
34284721	4	107	theme	composition	1050:1060	arg1	traits					1062:1067	milk composition traits	1045:1067	milk composition traits	1045:1067	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	4	108	theme	FT-MIR	859:864	arg1	phenotypes					877:886	895 individual FT-MIR wavenumber phenotypes	844:886	895 individual FT-MIR wavenumber phenotypes	844:886	RESULTS Separate GWAS conducted for each of 895 individual FT-MIR wavenumber phenotypes, identified 450 1-Mbp genomic regions with significant FT-MIR wavenumber QTL, compared to 246 1-Mbp genomic regions with QTL identified for FT-MIR predicted milk composition traits.					
34284721	5	109	theme	protein-sequence	1206:1221	arg1	mutations					1223:1231	31 protein-sequence mutations	1203:1231	31 protein-sequence mutations for FT-MIR wavenumber phenotypes	1203:1264	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	5	109	theme	protein-sequence	1206:1221	arg1	latter					1271:1276	latter	1271:1276	latter	1271:1276	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	7	110	from	clusters	1664:1671	arg1	composition					1775:1785	the chemical composition	1762:1785	the chemical composition of milk	1762:1793	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	5	111	theme	RNA-seq	1085:1091	arg1	data					1093:1096	mammary RNA-seq data	1077:1096	mammary RNA-seq data	1077:1096	Use of mammary RNA-seq data and gene annotation information identified 38 co-localized and co-segregating expression QTL (eQTL), and 31 protein-sequence mutations for FT-MIR wavenumber phenotypes, the latter including a null mutation in the ABO gene that has a potential role in changing milk oligosaccharide profiles.					
34284721	3	112	theme	dairy	541:545	arg1	cattle					547:552	38,085 mixed-breed New Zealand dairy cattle	510:552	38,085 mixed-breed New Zealand dairy cattle	510:552	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	7	113	theme	association	1590:1600	arg1	patterns					1602:1609	shared association patterns	1583:1609	shared association patterns for groups of genomically-distant loci	1583:1648	This revealed shared association patterns for groups of genomically-distant loci, highlighting clusters of loci linked through their biological roles in lactation and their presumed impacts on the chemical composition of milk.					
34284721	3	114	theme	New	529:531	arg1	Zealand					533:539	38,085 mixed-breed New Zealand	510:539	38,085 mixed-breed New Zealand dairy cattle	510:552	Using imputed whole-genome sequence for 38,085 mixed-breed New Zealand dairy cattle, we conducted GWAS on 895 individual FT-MIR wavenumber phenotypes, and assessed the value of these direct phenotypes for identifying candidate causal genes and variants, and improving our understanding of the physico-chemical properties of milk.					
34284721	6	115	theme	mid-infrared	1546:1557	arg1	spectrum					1559:1566	the mid-infrared spectrum	1542:1566	the mid-infrared spectrum	1542:1566	For the candidate causative genes implicated in these analyses, we examined the strength of association between relevant loci and each wavenumber across the mid-infrared spectrum.					
34284721	1	116	theme	novel	274:278	arg1	traits					280:285	other novel traits	268:285	other novel traits	268:285	BACKGROUND Fourier-transform mid-infrared (FT-MIR) spectroscopy provides a high-throughput and inexpensive method for predicting milk composition and other novel traits from milk samples.					
34284721	9	117	theme	milk	2254:2257	arg1	constituents					2259:2270	milk constituents	2254:2270	milk constituents	2254:2270	Examining patterns of significance across the mid-infrared spectrum for loci of interest further highlighted commonalities of association, which likely reflects the physico-chemical properties of milk constituents.					
34688788	6	0	theme	endoglucanase	1021:1033	arg1	numbers					997:1003	The gene numbers	988:1003	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti	988:1065	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	6	0	theme	endoglucanase	1021:1033	arg1	different					1076:1084	different	1076:1084	different	1076:1084	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	5	1	theme	Xylanibacillus	727:740	arg1	composti					742:749	Xylanibacillus composti	727:749	Xylanibacillus composti	727:749	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	6	2	theme	xylanase	1008:1015	arg1	numbers					997:1003	The gene numbers	988:1003	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti	988:1065	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	6	2	theme	xylanase	1008:1015	arg1	different					1076:1084	different	1076:1084	different	1076:1084	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	3	3	dep	thermophilic	332:343	arg1	xylanolytic					370:380	xylanolytic	370:380	xylanolytic	370:380	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	3	3	dep	thermophilic	332:343	arg1	anaerobic					359:367	anaerobic	359:367	anaerobic	359:367	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	2	4	theme	bioprocesses	314:325	arg1	construction					287:298	the construction	283:298	the construction of applicable bioprocesses	283:325	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	8	5	theme	high	1357:1360	arg1	expression					1362:1371	high expression	1357:1371	high expression of xylan and cellulose degradation genes	1357:1412	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	7	6	theme	Strain	1208:1213	arg1	DA-C8					1215:1219	Strain DA-C8	1208:1219	Strain DA-C8	1208:1219	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	7	7	theme	xylan	1238:1242	arg1	abilities					1270:1278	decreased xylan and corn hull degradation abilities	1228:1278	decreased xylan and corn hull degradation abilities	1228:1278	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	5	8	dep	information	850:860	arg1	properties					896:905	properties	896:905	properties	896:905	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	5	9	theme	identity	784:791	arg1	values					827:832	the average nucleotide identity and digital DNA-DNA hybridization values	761:832	the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties	761:905	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	6	10	theme	composti	1058:1065	arg1	numbers					997:1003	The gene numbers	988:1003	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti	988:1065	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	6	10	theme	composti	1058:1065	arg1	different					1076:1084	different	1076:1084	different	1076:1084	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	3	11	attach	isolated	476:483	arg1	compost					490:496	compost	490:496	compost	490:496	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	3	11	attach	isolated	476:483	arg2	=JCM34211=DSM111723					406:424	=JCM34211=DSM111723	406:424	=JCM34211=DSM111723	406:424	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	3	11	attach	isolated	476:483	arg2	DA-C8					399:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8	328:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation	328:464	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	8	12	theme	degradation	1396:1406	arg1	genes					1408:1412	xylan and cellulose degradation genes	1376:1412	xylan and cellulose degradation genes	1376:1412	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	5	13	theme	digital	797:803	arg1	hybridization					813:825	digital DNA-DNA hybridization	797:825	digital DNA-DNA hybridization	797:825	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	2	14	theme	bacteria	258:265	arg1	properties					219:228	the functional properties	204:228	the functional properties of cellulolytic xylanolytic bacteria	204:265	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	5	15	theme	gene	605:608	arg1	homology					619:626	16S rRNA gene sequence homology	596:626	16S rRNA gene sequence homology	596:626	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	9	16	theme	new	1618:1620	arg1	species					1622:1628	a new species	1616:1628	a new species of Paenibacillus with a unique polysaccharide degradation system	1616:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	9	16	theme	new	1618:1620	arg1	DA-C8					1607:1611	Strain DA-C8	1600:1611	Strain DA-C8	1600:1611	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	4	17	theme	1	532:532	arg1	%					533:533	%	533:533	%	533:533	Strain DA-C8 completely degraded 1% beechwood xylan within 4 days under anaerobic conditions.					
34688788	2	18	theme	cellulolytic	233:244	arg1	bacteria					258:265	cellulolytic xylanolytic bacteria	233:265	cellulolytic xylanolytic bacteria	233:265	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	1	19	theme	anaerobic	138:146	arg1	conditions					148:157	anaerobic conditions	138:157	anaerobic conditions	138:157	strain DA-C8 that exhibits xylan degradation under anaerobic conditions.					
34688788	6	20	theme	strain	1038:1043	arg1	DA-C8					1045:1049	strain DA-C8	1038:1049	strain DA-C8	1038:1049	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	5	21	theme	average	765:771	arg1	identity					784:791	average nucleotide identity	765:791	average nucleotide identity	765:791	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	6	22	theme	higher	1113:1118	arg1	abundance					1120:1128	higher abundance	1113:1128	higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase	1113:1188	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	3	23	theme	bacterial	382:390	arg1	=JCM34211=DSM111723					406:424	=JCM34211=DSM111723	406:424	=JCM34211=DSM111723	406:424	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	3	23	theme	bacterial	382:390	arg1	DA-C8					399:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8	328:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation	328:464	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	4	24	theme	Strain	499:504	arg1	DA-C8					506:510	Strain DA-C8	499:510	Strain DA-C8	499:510	Strain DA-C8 completely degraded 1% beechwood xylan within 4 days under anaerobic conditions.					
34688788	0	25	theme	sp	83:84	arg1	Characterization					0:15	Characterization	0:15	Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp.	0:85	Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp.					
34688788	5	26	theme	16S	596:598	arg1	rRNA					600:603	16S rRNA	596:603	16S rRNA gene sequence homology	596:626	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	9	27	theme	unique	1654:1659	arg1	system					1688:1693	a unique polysaccharide degradation system	1652:1693	a unique polysaccharide degradation system	1652:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	5	28	theme	strain	660:665	arg1	DA-C8					667:671	strain DA-C8	660:671	strain DA-C8	660:671	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	9	29	theme	degradation	1676:1686	arg1	system					1688:1693	a unique polysaccharide degradation system	1652:1693	a unique polysaccharide degradation system	1652:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	4	30	theme	anaerobic	571:579	arg1	conditions					581:590	anaerobic conditions	571:590	anaerobic conditions	571:590	Strain DA-C8 completely degraded 1% beechwood xylan within 4 days under anaerobic conditions.					
34688788	7	31	theme	aerobic	1313:1319	arg1	conditions					1321:1330	aerobic conditions	1313:1330	aerobic conditions	1313:1330	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	4	32	theme	beechwood	535:543	arg1	xylan					545:549	1% beechwood xylan	532:549	1% beechwood xylan	532:549	Strain DA-C8 completely degraded 1% beechwood xylan within 4 days under anaerobic conditions.					
34688788	8	33	theme	aerobic	1477:1483	arg1	conditions					1485:1494	aerobic conditions	1477:1494	aerobic conditions	1477:1494	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	5	34	theme	phylogenetic	632:643	arg1	analysis					650:657	phylogenetic tree analysis	632:657	phylogenetic tree analysis	632:657	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	6	35	theme	β-glucosidase	1176:1188	arg1	abundance					1120:1128	higher abundance	1113:1128	higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase	1113:1188	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	3	36	theme	xylan	448:452	arg1	degradation					454:464	efficient xylan degradation	438:464	efficient xylan degradation	438:464	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	9	37	with	species	1622:1628	arg1	system					1688:1693	a unique polysaccharide degradation system	1652:1693	a unique polysaccharide degradation system	1652:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	5	38	theme	strain	922:927	arg1	DA-C8					929:933	strain DA-C8	922:933	strain DA-C8	922:933	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	7	39	theme	degradation	1258:1268	arg1	abilities					1270:1278	decreased xylan and corn hull degradation abilities	1228:1278	decreased xylan and corn hull degradation abilities	1228:1278	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	5	40	theme	carbon	870:875	arg1	utilization					884:894	the carbon source utilization	866:894	the carbon source utilization	866:894	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	5	41	theme	genome	843:848	arg1	information					850:860	genome information	843:860	genome information	843:860	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	8	42	theme	polysaccharide	1535:1548	arg1	degradation					1550:1560	polysaccharide degradation	1535:1560	polysaccharide degradation	1535:1560	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	7	43	theme	corn	1248:1251	arg1	degradation					1258:1268	corn hull degradation	1248:1268	corn hull degradation	1248:1268	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	2	44	theme	properties	219:228	arg1	study					195:199	study	195:199	study	195:199	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	2	44	theme	properties	219:228	arg1	screening					164:172	screening	164:172	screening	164:172	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	2	44	theme	properties	219:228	arg1	identification					175:188	identification	175:188	identification	175:188	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	0	45	theme	Paenibacillus	69:81	arg1	sp					83:84	a thermophilic facultatively anaerobic bacterium Paenibacillus sp	20:84	a thermophilic facultatively anaerobic bacterium Paenibacillus sp	20:84	Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp.					
34688788	7	46	theme	decreased	1228:1236	arg1	abilities					1270:1278	decreased xylan and corn hull degradation abilities	1228:1278	decreased xylan and corn hull degradation abilities	1228:1278	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	6	47	theme	X.	1195:1196	arg1	composti					1198:1205	X. composti	1195:1205	X. composti	1195:1205	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	2	48	theme	applicable	303:312	arg1	bioprocesses					314:325	applicable bioprocesses	303:325	applicable bioprocesses	303:325	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	0	49	theme	bacterium	59:67	arg1	sp					83:84	a thermophilic facultatively anaerobic bacterium Paenibacillus sp	20:84	a thermophilic facultatively anaerobic bacterium Paenibacillus sp	20:84	Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp.					
34688788	6	50	theme	gene	992:995	arg1	numbers					997:1003	The gene numbers	988:1003	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti	988:1065	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	6	50	theme	gene	992:995	arg1	different					1076:1084	different	1076:1084	different	1076:1084	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	5	51	theme	Paenibacillus	696:708	arg1	cisolokensis					710:721	Paenibacillus cisolokensis	696:721	Paenibacillus cisolokensis	696:721	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	5	52	theme	nucleotide	773:782	arg1	identity					784:791	average nucleotide identity	765:791	average nucleotide identity	765:791	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	8	53	theme	xylan	1376:1380	arg1	genes					1408:1412	xylan and cellulose degradation genes	1376:1412	xylan and cellulose degradation genes	1376:1412	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	9	54	theme	Strain	1600:1605	arg1	species					1622:1628	a new species	1616:1628	a new species of Paenibacillus with a unique polysaccharide degradation system	1616:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	9	54	theme	Strain	1600:1605	arg1	DA-C8					1607:1611	Strain DA-C8	1600:1611	Strain DA-C8	1600:1611	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	6	55	theme	X.	1055:1056	arg1	composti					1058:1065	X. composti	1055:1065	X. composti	1055:1065	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	4	56	theme	%	533:533	arg1	xylan					545:549	1% beechwood xylan	532:549	1% beechwood xylan	532:549	Strain DA-C8 completely degraded 1% beechwood xylan within 4 days under anaerobic conditions.					
34688788	8	57	theme	cellulose	1386:1394	arg1	degradation					1396:1406	cellulose degradation	1386:1406	cellulose degradation	1386:1406	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	5	58	theme	sequence	610:617	arg1	homology					619:626	16S rRNA gene sequence homology	596:626	16S rRNA gene sequence homology	596:626	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	8	59	theme	Quantitative	1333:1344	arg1	PCR					1346:1348	Quantitative PCR	1333:1348	Quantitative PCR	1333:1348	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	6	60	theme	DA-C8	1045:1049	arg1	numbers					997:1003	The gene numbers	988:1003	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti	988:1065	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	6	60	theme	DA-C8	1045:1049	arg1	different					1076:1084	different	1076:1084	different	1076:1084	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	8	61	theme	genes	1408:1412	arg1	expression					1362:1371	high expression	1357:1371	high expression of xylan and cellulose degradation genes	1357:1412	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	9	62	theme	Paenibacillus	1633:1645	arg1	species					1622:1628	a new species	1616:1628	a new species of Paenibacillus with a unique polysaccharide degradation system	1616:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	9	62	theme	Paenibacillus	1633:1645	arg1	DA-C8					1607:1611	Strain DA-C8	1600:1611	Strain DA-C8	1600:1611	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	3	63	theme	thermophilic	332:343	arg1	=JCM34211=DSM111723					406:424	=JCM34211=DSM111723	406:424	=JCM34211=DSM111723	406:424	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	3	63	theme	thermophilic	332:343	arg1	DA-C8					399:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8	328:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation	328:464	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	8	64	theme	anaerobic	1420:1428	arg1	conditions					1430:1439	anaerobic conditions	1420:1439	anaerobic conditions	1420:1439	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	3	65	theme	strain	392:397	arg1	=JCM34211=DSM111723					406:424	=JCM34211=DSM111723	406:424	=JCM34211=DSM111723	406:424	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	3	65	theme	strain	392:397	arg1	DA-C8					399:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8	328:403	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation	328:464	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	5	66	theme	hybridization	813:825	arg1	values					827:832	the average nucleotide identity and digital DNA-DNA hybridization values	761:832	the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties	761:905	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	5	67	theme	rRNA	600:603	arg1	homology					619:626	16S rRNA gene sequence homology	596:626	16S rRNA gene sequence homology	596:626	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	9	68	theme	polysaccharide	1661:1674	arg1	system					1688:1693	a unique polysaccharide degradation system	1652:1693	a unique polysaccharide degradation system	1652:1693	Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system.					
34688788	6	69	theme	strain	1096:1101	arg1	DA-C8					1103:1107	strain DA-C8	1096:1107	strain DA-C8	1096:1107	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	5	70	theme	DNA-DNA	805:811	arg1	hybridization					813:825	digital DNA-DNA hybridization	797:825	digital DNA-DNA hybridization	797:825	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	6	71	contain	had	1109:1111	arg2	abundance					1120:1128	higher abundance	1113:1128	higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase	1113:1188	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	6	71	contain	had	1109:1111	arg1	DA-C8					1103:1107	strain DA-C8	1096:1107	strain DA-C8	1096:1107	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	7	72	theme	xylan	1294:1298	arg1	medium					1300:1305	xylan medium	1294:1305	xylan medium	1294:1305	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	2	73	dep	cellulolytic	233:244	arg1	xylanolytic					246:256	xylanolytic	246:256	xylanolytic	246:256	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	1	74	theme	strain	87:92	arg1	DA-C8					94:98	strain DA-C8	87:98	strain DA-C8 that exhibits xylan degradation under anaerobic conditions	87:157	strain DA-C8 that exhibits xylan degradation under anaerobic conditions.					
34688788	5	75	theme	tree	645:648	arg1	analysis					650:657	phylogenetic tree analysis	632:657	phylogenetic tree analysis	632:657	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	2	76	dep	screening	164:172	arg1	The					160:162	The	160:162	The	160:162	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
34688788	8	77	theme	aeration	1579:1586	arg1	conditions					1588:1597	the aeration conditions	1575:1597	the aeration conditions	1575:1597	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	0	78	theme	thermophilic	22:33	arg1	sp					83:84	a thermophilic facultatively anaerobic bacterium Paenibacillus sp	20:84	a thermophilic facultatively anaerobic bacterium Paenibacillus sp	20:84	Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp.					
34688788	1	79	theme	xylan	114:118	arg1	degradation					120:130	xylan degradation	114:130	xylan degradation	114:130	strain DA-C8 that exhibits xylan degradation under anaerobic conditions.					
34688788	6	80	theme	β-xylosidase	1158:1169	arg1	abundance					1120:1128	higher abundance	1113:1128	higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase	1113:1188	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	3	81	theme	efficient	438:446	arg1	degradation					454:464	efficient xylan degradation	438:464	efficient xylan degradation	438:464	The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost.					
34688788	7	82	theme	hull	1253:1256	arg1	degradation					1258:1268	corn hull degradation	1248:1268	corn hull degradation	1248:1268	Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions.					
34688788	6	83	theme	α-L-arabinofuranosidase	1133:1155	arg1	abundance					1120:1128	higher abundance	1113:1128	higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase	1113:1188	The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti.					
34688788	8	84	theme	strain	1513:1518	arg1	DA-C8					1520:1524	strain DA-C8	1513:1524	strain DA-C8	1513:1524	Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions.					
34688788	5	85	theme	source	877:882	arg1	utilization					884:894	the carbon source utilization	866:894	the carbon source utilization	866:894	By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus.					
34688788	0	86	dep	thermophilic	22:33	arg1	anaerobic					49:57	anaerobic	49:57	anaerobic	49:57	Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp.					
34688788	2	87	theme	functional	208:217	arg1	properties					219:228	the functional properties	204:228	the functional properties of cellulolytic xylanolytic bacteria	204:265	The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses.					
33142626	8	0	theme	AC	994:995	arg1	modification					978:989	the modification	974:989	the modification of AC by chitosan and Fe3O4 nanoparticles	974:1031	The acquired findings revealed that the modification of AC by chitosan and Fe3O4 nanoparticles increased the Cr adsorption.					
33142626	9	1	dep	re-used	1072:1078	arg1	indicates					1121:1129	indicates	1121:1129	indicates that the composites are a promising option for Cr removal	1121:1187	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	2	2	theme	adsorbers	343:351	arg1	properties					329:338	the properties	325:338	the properties of adsorbers to absorb chromium ions	325:375	Various techniques were applied to reveal the properties of adsorbers to absorb chromium ions.					
33142626	1	3	theme	activated	247:255	arg1	carbon					257:262	Moringa oleifera activated carbon	230:262	Moringa oleifera activated carbon (AC)	230:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	1	3	theme	activated	247:255	arg1	AC					265:266	AC	265:266	AC	265:266	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	0	4	theme	carbon	75:80	arg1	behavior					36:43	the chromium removal behavior	15:43	the chromium removal behavior of Moringa oleifera activated carbon	15:80	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	4	5	theme	process	481:487	arg1	behavior					449:456	The kinetic behavior	437:456	The kinetic behavior of the chromium uptake process	437:487	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	1	6	theme	chitosan	149:156	arg1	nanoparticles					177:189	chitosan and Fe3O4 magnetic nanoparticles	149:189	chitosan and Fe3O4 magnetic nanoparticles	149:189	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	1	7	theme	carbon	257:262	arg1	property					218:225	the chromium adsorption property	194:225	the chromium adsorption property of Moringa oleifera activated carbon (AC)	194:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	4	8	theme	uptake	474:479	arg1	process					481:487	the chromium uptake process	461:487	the chromium uptake process	461:487	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	6	9	theme	mg/g	775:778	arg1	capacity					706:713	The maximum single-layer adsorption capacity	670:713	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	670:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	9	theme	mg/g	775:778	arg1	mg/g					801:804	56.78 mg/g, 114.80 mg/g, 121.70 mg/g	769:804	mg/g	801:804	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	4	10	theme	kinetic	441:447	arg1	behavior					449:456	The kinetic behavior	437:456	The kinetic behavior of the chromium uptake process	437:487	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	5	11	theme	thermodynamic	565:577	arg1	investigation					579:591	the thermodynamic investigation	561:591	the thermodynamic investigation	561:591	Based on the thermodynamic investigation, the chromium adsorption using all adsorbers was exothermic and spontaneous.					
33142626	6	12	theme	adsorption	695:704	arg1	capacity					706:713	The maximum single-layer adsorption capacity	670:713	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	670:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	12	theme	adsorption	695:704	arg1	mg/g					801:804	56.78 mg/g, 114.80 mg/g, 121.70 mg/g	769:804	mg/g	801:804	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	8	13	theme	Fe3O4	1013:1017	arg1	nanoparticles					1019:1031	Fe3O4 nanoparticles	1013:1031	Fe3O4 nanoparticles	1013:1031	The acquired findings revealed that the modification of AC by chitosan and Fe3O4 nanoparticles increased the Cr adsorption.					
33142626	1	14	from	effect	139:144	arg1	property					218:225	the chromium adsorption property	194:225	the chromium adsorption property of Moringa oleifera activated carbon (AC)	194:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	6	15	theme	single-layer	682:693	arg1	capacity					706:713	The maximum single-layer adsorption capacity	670:713	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	670:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	15	theme	single-layer	682:693	arg1	mg/g					801:804	56.78 mg/g, 114.80 mg/g, 121.70 mg/g	769:804	mg/g	801:804	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	0	16	theme	iron	98:101	arg1	oxide					103:107	iron oxide	98:107	iron oxide	98:107	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	7	17	theme	Cr-adsorption	891:903	arg1	optimal					916:922	optimal	916:922	optimal	916:922	The isotherm constants quantity highlighted that the Cr-adsorption process is optimal and physical.					
33142626	7	17	theme	Cr-adsorption	891:903	arg1	process					905:911	the Cr-adsorption process	887:911	the Cr-adsorption process	887:911	The isotherm constants quantity highlighted that the Cr-adsorption process is optimal and physical.					
33142626	6	18	theme	maximum	674:680	arg1	capacity					706:713	The maximum single-layer adsorption capacity	670:713	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	670:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	18	theme	maximum	674:680	arg1	mg/g					801:804	56.78 mg/g, 114.80 mg/g, 121.70 mg/g	769:804	mg/g	801:804	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	1	19	theme	Fe3O4	162:166	arg1	nanoparticles					177:189	chitosan and Fe3O4 magnetic nanoparticles	149:189	chitosan and Fe3O4 magnetic nanoparticles	149:189	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	5	20	theme	chromium	598:605	arg1	exothermic					642:651	exothermic	642:651	exothermic	642:651	Based on the thermodynamic investigation, the chromium adsorption using all adsorbers was exothermic and spontaneous.					
33142626	5	20	theme	chromium	598:605	arg1	adsorption					607:616	the chromium adsorption	594:616	the chromium adsorption using all adsorbers	594:636	Based on the thermodynamic investigation, the chromium adsorption using all adsorbers was exothermic and spontaneous.					
33142626	6	21	theme	adsorbers	755:763	arg1	capacity					706:713	The maximum single-layer adsorption capacity	670:713	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	670:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	21	theme	adsorbers	755:763	arg1	mg/g					801:804	56.78 mg/g, 114.80 mg/g, 121.70 mg/g	769:804	mg/g	801:804	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	1	22	theme	magnetic	168:175	arg1	nanoparticles					177:189	chitosan and Fe3O4 magnetic nanoparticles	149:189	chitosan and Fe3O4 magnetic nanoparticles	149:189	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	4	23	theme	kinetic	529:535	arg1	PSOKM					544:548	PSOKM	544:548	PSOKM	544:548	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	4	23	theme	kinetic	529:535	arg1	model					537:541	the pseudo-second-order kinetic model	505:541	the pseudo-second-order kinetic model (PSOKM)	505:549	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	6	24	theme	CS/AC/Fe3O4	743:753	arg1	adsorbers					755:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	2	25	theme	chromium	363:370	arg1	ions					372:375	absorb chromium ions	356:375	absorb chromium ions	356:375	Various techniques were applied to reveal the properties of adsorbers to absorb chromium ions.					
33142626	0	26	theme	chromium	19:26	arg1	behavior					36:43	the chromium removal behavior	15:43	the chromium removal behavior of Moringa oleifera activated carbon	15:80	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	4	27	theme	pseudo-second-order	509:527	arg1	PSOKM					544:548	PSOKM	544:548	PSOKM	544:548	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	4	27	theme	pseudo-second-order	509:527	arg1	model					537:541	the pseudo-second-order kinetic model	505:541	the pseudo-second-order kinetic model (PSOKM)	505:549	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	8	28	theme	Cr	1047:1048	arg1	adsorption					1050:1059	the Cr adsorption	1043:1059	the Cr adsorption	1043:1059	The acquired findings revealed that the modification of AC by chitosan and Fe3O4 nanoparticles increased the Cr adsorption.					
33142626	7	29	dep	highlighted	870:880	arg1	quantity					861:868	quantity	861:868	quantity	861:868	The isotherm constants quantity highlighted that the Cr-adsorption process is optimal and physical.					
33142626	9	30	theme	>90	1108:1110	arg1	%					1112:1112	%	1112:1112	%	1112:1112	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	2	31	theme	Various	283:289	arg1	techniques					291:300	Various techniques	283:300	Various techniques	283:300	Various techniques were applied to reveal the properties of adsorbers to absorb chromium ions.					
33142626	0	32	theme	behavior	36:43	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide	0:107	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	1	33	theme	nanoparticles	177:189	arg1	effect					139:144	The effect	135:144	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC)	135:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	1	34	theme	chromium	198:205	arg1	property					218:225	the chromium adsorption property	194:225	the chromium adsorption property of Moringa oleifera activated carbon (AC)	194:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	0	35	theme	removal	28:34	arg1	behavior					36:43	the chromium removal behavior	15:43	the chromium removal behavior of Moringa oleifera activated carbon	15:80	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	3	36	theme	pollutant	396:404	arg1	majority					380:387	A majority	378:387	A majority of the pollutant	378:404	A majority of the pollutant was removed at pH2 and 50 min.					
33142626	9	37	theme	Cr	1178:1179	arg1	removal					1181:1187	Cr removal	1178:1187	Cr removal	1178:1187	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	1	38	theme	adsorption	207:216	arg1	property					218:225	the chromium adsorption property	194:225	the chromium adsorption property of Moringa oleifera activated carbon (AC)	194:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	0	39	theme	Moringa	48:54	arg1	carbon					75:80	Moringa oleifera activated carbon	48:80	Moringa oleifera activated carbon	48:80	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	6	40	theme	AC/Fe3O4	729:736	arg1	adsorbers					755:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	9	41	theme	promising	1157:1165	arg1	option					1167:1172	a promising option	1155:1172	a promising option for Cr removal	1155:1187	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	9	41	theme	promising	1157:1165	arg1	composites					1140:1149	the composites	1136:1149	the composites	1136:1149	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	6	42	theme	CS/AC	722:726	arg1	adsorbers					755:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	8	43	theme	acquired	942:949	arg1	findings					951:958	The acquired findings	938:958	The acquired findings	938:958	The acquired findings revealed that the modification of AC by chitosan and Fe3O4 nanoparticles increased the Cr adsorption.					
33142626	7	44	theme	isotherm	842:849	arg1	constants					851:859	The isotherm constants	838:859	The isotherm constants	838:859	The isotherm constants quantity highlighted that the Cr-adsorption process is optimal and physical.					
33142626	9	45	dep	5	1086:1086	arg1	to					1083:1084	to	1083:1084	to	1083:1084	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	0	46	theme	activated	65:73	arg1	carbon					75:80	Moringa oleifera activated carbon	48:80	Moringa oleifera activated carbon	48:80	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	6	47	theme	mg/g	788:791	arg1	capacity					706:713	The maximum single-layer adsorption capacity	670:713	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	670:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	47	theme	mg/g	788:791	arg1	mg/g					801:804	56.78 mg/g, 114.80 mg/g, 121.70 mg/g	769:804	mg/g	801:804	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	6	48	theme	AC	718:719	arg1	adsorbers					755:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers	718:763	The maximum single-layer adsorption capacity of AC, CS/AC, AC/Fe3O4, and CS/AC/Fe3O4 adsorbers was 56.78 mg/g, 114.80 mg/g, 121.70 mg/g, and 130.80 mg/g, respectively.					
33142626	2	49	theme	absorb	356:361	arg1	ions					372:375	absorb chromium ions	356:375	absorb chromium ions	356:375	Various techniques were applied to reveal the properties of adsorbers to absorb chromium ions.					
33142626	1	50	theme	Moringa	230:236	arg1	carbon					257:262	Moringa oleifera activated carbon	230:262	Moringa oleifera activated carbon (AC)	230:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	1	50	theme	Moringa	230:236	arg1	AC					265:266	AC	265:266	AC	265:266	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	0	51	theme	oleifera	56:63	arg1	carbon					75:80	Moringa oleifera activated carbon	48:80	Moringa oleifera activated carbon	48:80	Enhancement of the chromium removal behavior of Moringa oleifera activated carbon by chitosan and iron oxide nanoparticles from water.					
33142626	4	52	theme	chromium	465:472	arg1	process					481:487	the chromium uptake process	461:487	the chromium uptake process	461:487	The kinetic behavior of the chromium uptake process was governed by the pseudo-second-order kinetic model (PSOKM).					
33142626	9	53	theme	%	1112:1112	arg1	yield					1102:1106	a yield	1100:1106	a yield >90 %	1100:1112	Adsorbers re-used up to 5 stages with a yield >90 %, which indicates that the composites are a promising option for Cr removal.					
33142626	1	54	theme	oleifera	238:245	arg1	carbon					257:262	Moringa oleifera activated carbon	230:262	Moringa oleifera activated carbon (AC)	230:267	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
33142626	1	54	theme	oleifera	238:245	arg1	AC					265:266	AC	265:266	AC	265:266	The effect of chitosan and Fe3O4 magnetic nanoparticles on the chromium adsorption property of Moringa oleifera activated carbon (AC) was explored.					
32385377	7	0	theme	useful	1267:1272	arg1	information					1274:1284	useful information	1267:1284	useful information for genetic improvement and management strategies in dairy cattle	1267:1350	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	3	1	theme	dependency	399:408	arg1	relationships					410:422	interrelated dependency relationships	386:422	interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N)	386:581	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	0	2	theme	health	89:94	arg1	architecture					67:78	multi-trait genetic architecture	47:78	multi-trait genetic architecture of udder health in dairy cattle	47:110	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	3	3	theme	milk	575:578	arg1	N					580:580	total milk N	569:580	total milk N	569:580	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	7	4	from	improvement	1298:1308	arg1	cattle					1345:1350	dairy cattle	1339:1350	dairy cattle	1339:1350	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	1	5	theme	costly	155:160	arg1	diseases					162:169	costly diseases	155:169	costly diseases	155:169	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	3	6	theme	N	580:580	arg1	%					564:564	%	564:564	% of total milk N	564:580	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	6	theme	N	580:580	arg1	NCN					559:561	NCN	559:561	NCN	559:561	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	6	theme	N	580:580	arg1	N					580:580	total milk N	569:580	total milk N	569:580	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	2	7	theme	inflammation	272:283	arg1	indicators					252:261	indicators	252:261	indicators of udder inflammation	252:283	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	2	7	theme	inflammation	272:283	arg1	changes					202:208	changes	202:208	changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs	202:312	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	7	8	theme	SNP	1244:1246	arg1	effects					1248:1254	SNP effects	1244:1254	SNP effects	1244:1254	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	0	9	theme	dairy	99:103	arg1	cattle					105:110	dairy cattle	99:110	dairy cattle	99:110	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	0	10	from	architecture	67:78	arg1	cattle					105:110	dairy cattle	99:110	dairy cattle	99:110	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	6	11	theme	membrane	1055:1062	arg1	activity					1074:1081	membrane transport activity	1055:1081	membrane transport activity	1055:1081	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	2	12	theme	udder	266:270	arg1	inflammation					272:283	udder inflammation	266:283	udder inflammation	266:283	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	4	13	used	used	691:694	arg2	network					638:644	The phenotypic network	623:644	The phenotypic network inferred via the Hill-Climbing algorithm	623:685	The phenotypic network inferred via the Hill-Climbing algorithm was used to estimate SEM parameters.					
32385377	7	14	theme	effects	1248:1254	arg1	path					1236:1239	the path	1232:1239	the path of SNP effects	1232:1254	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	3	15	theme	structural	326:335	arg1	SEM					356:358	SEM	356:358	SEM	356:358	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	15	theme	structural	326:335	arg1	modeling					346:353	structural equation modeling	326:353	structural equation modeling (SEM)	326:359	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	5	16	theme	significant	791:801	arg1	SNPs					803:806	six significant SNPs	787:806	six significant SNPs for SCS	787:814	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	4	17	theme	SEM	708:710	arg1	parameters					712:721	SEM parameters	708:721	SEM parameters	708:721	The phenotypic network inferred via the Hill-Climbing algorithm was used to estimate SEM parameters.					
32385377	1	18	from	prevalent	141:149	arg1	cattle					180:185	dairy cattle	174:185	dairy cattle	174:185	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	3	19	theme	somatic	493:499	arg1	score					506:510	somatic cell score	493:510	somatic cell score (SCS)	493:516	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	19	theme	somatic	493:499	arg1	SCS					513:515	SCS	513:515	SCS	513:515	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	2	20	from	changes	202:208	arg1	quality					234:240	quality	234:240	quality	234:240	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	2	20	from	changes	202:208	arg1	composition					218:228	milk composition	213:228	milk composition	213:228	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	1	21	theme	diseases	162:169	arg1	prevalent					141:149	prevalent	141:149	prevalent	141:149	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	1	21	theme	diseases	162:169	arg1	diseases					162:169	costly diseases	155:169	costly diseases	155:169	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	1	21	theme	diseases	162:169	arg1	one					125:127	one	125:127	one	125:127	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	3	22	theme	equation	337:344	arg1	SEM					356:358	SEM	356:358	SEM	356:358	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	22	theme	equation	337:344	arg1	modeling					346:353	structural equation modeling	326:353	structural equation modeling (SEM)	326:359	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	7	23	theme	genetic	1290:1296	arg1	improvement					1298:1308	genetic improvement	1290:1308	genetic improvement	1290:1308	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	7	24	from	strategies	1325:1334	arg1	cattle					1345:1350	dairy cattle	1339:1350	dairy cattle	1339:1350	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	7	25	theme	new	1161:1163	arg1	insights					1165:1172	new insights	1161:1172	new insights on the relationships among udder health phenotypes and on the path of SNP effects	1161:1254	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	3	26	theme	cell	501:504	arg1	score					506:510	somatic cell score	493:510	somatic cell score (SCS)	493:516	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	26	theme	cell	501:504	arg1	SCS					513:515	SCS	513:515	SCS	513:515	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	6	27	theme	overrepresented	946:960	arg1	pathways					962:969	overrepresented pathways	946:969	overrepresented pathways	946:969	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	0	28	theme	equation	11:18	arg1	modeling					20:27	Structural equation modeling	0:27	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.	0:111	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	2	29	theme	clinical	299:306	arg1	signs					308:312	clinical signs	299:312	clinical signs	299:312	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	7	30	theme	management	1314:1323	arg1	strategies					1325:1334	management strategies	1314:1334	management strategies	1314:1334	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	1	31	theme	dairy	174:178	arg1	cattle					180:185	dairy cattle	174:185	dairy cattle	174:185	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	0	32	theme	Structural	0:9	arg1	modeling					20:27	Structural equation modeling	0:27	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.	0:111	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	3	33	theme	Swiss	611:615	arg1	cows					617:620	1,158 Brown Swiss cows	599:620	1,158 Brown Swiss cows	599:620	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	7	34	theme	health	1207:1212	arg1	phenotypes					1214:1223	udder health phenotypes	1201:1223	udder health phenotypes	1201:1223	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	5	35	theme	models-GWAS	751:761	arg1	Integration					724:734	Integration	724:734	Integration of multi-trait models-GWAS and SEM-GWAS	724:774	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	3	36	theme	cows	617:620	arg1	cohort					589:594	a cohort	587:594	a cohort of 1,158 Brown Swiss cows	587:620	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	6	37	theme	biological	1027:1036	arg1	knowledge					1038:1046	biological knowledge	1027:1046	biological knowledge	1027:1046	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	3	38	theme	udder	452:456	arg1	health					458:463	udder health	452:463	udder health	452:463	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	5	39	theme	MY	852:853	arg1	contribution					836:847	the contribution	832:847	the contribution of MY and LACT acting as mediator traits to total SNP effects	832:909	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	1	40	dep	prevalent	141:149	arg1	the					132:134	the	132:134	the	132:134	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	3	41	dep	lactose	519:525	arg1	LACT					531:534	LACT	531:534	LACT	531:534	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	41	dep	lactose	519:525	arg1	%					528:528	%	528:528	%	528:528	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	6	42	theme	Functional	912:921	arg1	analyses					923:930	Functional analyses	912:930	Functional analyses	912:930	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	0	43	theme	multi-trait	47:57	arg1	architecture					67:78	multi-trait genetic architecture	47:78	multi-trait genetic architecture of udder health in dairy cattle	47:110	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	3	44	theme	Brown	605:609	arg1	cows					617:620	1,158 Brown Swiss cows	599:620	1,158 Brown Swiss cows	599:620	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	6	45	with	consistent	1011:1020	arg1	knowledge					1038:1046	biological knowledge	1027:1046	biological knowledge	1027:1046	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	3	46	theme	related	441:447	arg1	N					556:556	non-casein N	545:556	non-casein N (NCN, % of total milk N)	545:581	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	46	theme	related	441:447	arg1	phenotypes					430:439	phenotypes	430:439	phenotypes	430:439	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	46	theme	related	441:447	arg1	lactose					519:525	lactose	519:525	lactose (%, LACT)	519:535	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	46	theme	related	441:447	arg1	yield					481:485	milk yield	476:485	milk yield (MY)	476:490	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	46	theme	related	441:447	arg1	pH					538:539	pH	538:539	pH	538:539	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	46	theme	related	441:447	arg1	score					506:510	somatic cell score	493:510	somatic cell score (SCS)	493:516	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	47	theme	interrelated	386:397	arg1	relationships					410:422	interrelated dependency relationships	386:422	interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N)	386:581	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	5	48	theme	multi-trait	739:749	arg1	models-GWAS					751:761	multi-trait models-GWAS	739:761	multi-trait models-GWAS	739:761	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	3	49	theme	non-casein	545:554	arg1	N					556:556	non-casein N	545:556	non-casein N (NCN, % of total milk N)	545:581	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	2	50	theme	milk	213:216	arg1	composition					218:228	milk composition	213:228	milk composition	213:228	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	5	51	theme	total	893:897	arg1	effects					903:909	total SNP effects	893:909	total SNP effects	893:909	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	3	52	theme	milk	476:479	arg1	MY					488:489	MY	488:489	MY	488:489	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	52	theme	milk	476:479	arg1	yield					481:485	milk yield	476:485	milk yield (MY)	476:490	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	0	53	theme	genetic	59:65	arg1	architecture					67:78	multi-trait genetic architecture	47:78	multi-trait genetic architecture of udder health in dairy cattle	47:110	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	5	54	theme	SNP	899:901	arg1	effects					903:909	total SNP effects	893:909	total SNP effects	893:909	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	6	55	dep	consistent	1011:1020	arg1	activity					1074:1081	membrane transport activity	1055:1081	membrane transport activity	1055:1081	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	7	56	theme	udder	1201:1205	arg1	phenotypes					1214:1223	udder health phenotypes	1201:1223	udder health phenotypes	1201:1223	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	3	57	theme	total	569:573	arg1	N					580:580	total milk N	569:580	total milk N	569:580	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	5	58	theme	LACT	859:862	arg1	contribution					836:847	the contribution	832:847	the contribution of MY and LACT acting as mediator traits to total SNP effects	832:909	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	6	59	theme	Wnt	1100:1102	arg1	signaling					1104:1112	Wnt signaling	1100:1112	Wnt signaling	1100:1112	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	2	60	from	indicators	252:261	arg1	absence					288:294	absence	288:294	absence of clinical signs	288:312	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	4	61	theme	phenotypic	627:636	arg1	network					638:644	The phenotypic network	623:644	The phenotypic network inferred via the Hill-Climbing algorithm	623:685	The phenotypic network inferred via the Hill-Climbing algorithm was used to estimate SEM parameters.					
32385377	6	62	theme	transport	1064:1072	arg1	activity					1074:1081	membrane transport activity	1055:1081	membrane transport activity	1055:1081	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	7	63	from	insights	1165:1172	arg1	relationships					1181:1193	the relationships	1177:1193	the relationships among udder health phenotypes	1177:1223	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	7	63	from	insights	1165:1172	arg1	path					1236:1239	the path	1232:1239	the path of SNP effects	1232:1254	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	2	64	theme	signs	308:312	arg1	absence					288:294	absence	288:294	absence of clinical signs	288:312	It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs.					
32385377	3	65	dep	N	556:556	arg1	%					564:564	%	564:564	% of total milk N	564:580	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	65	dep	N	556:556	arg1	NCN					559:561	NCN	559:561	NCN	559:561	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	3	65	dep	N	556:556	arg1	N					580:580	total milk N	569:580	total milk N	569:580	We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows.					
32385377	0	66	theme	udder	83:87	arg1	health					89:94	udder health	83:94	udder health	83:94	Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle.					
32385377	4	67	theme	Hill-Climbing	663:675	arg1	algorithm					677:685	the Hill-Climbing algorithm	659:685	the Hill-Climbing algorithm	659:685	The phenotypic network inferred via the Hill-Climbing algorithm was used to estimate SEM parameters.					
32385377	1	68	from	cattle	180:185	arg1	prevalent					141:149	prevalent	141:149	prevalent	141:149	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	5	69	theme	SEM-GWAS	767:774	arg1	Integration					724:734	Integration	724:734	Integration of multi-trait models-GWAS and SEM-GWAS	724:774	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	6	70	dep	activity	1074:1081	arg1	e.g.					1049:1052	e.g.	1049:1052	e.g.	1049:1052	Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN).					
32385377	7	71	theme	dairy	1339:1343	arg1	cattle					1345:1350	dairy cattle	1339:1350	dairy cattle	1339:1350	In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.					
32385377	5	72	theme	mediator	874:881	arg1	traits					883:888	mediator traits	874:888	mediator traits	874:888	Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects.					
32385377	1	73	theme	prevalent	141:149	arg1	prevalent					141:149	prevalent	141:149	prevalent	141:149	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	1	73	theme	prevalent	141:149	arg1	diseases					162:169	costly diseases	155:169	costly diseases	155:169	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32385377	1	73	theme	prevalent	141:149	arg1	one					125:127	one	125:127	one	125:127	Mastitis is one of the most prevalent and costly diseases in dairy cattle.					
32512098	7	0	theme	ΔH	1030:1031	arg1	values					1020:1025	The negative values	1007:1025	The negative values of ΔH and ΔF accompanying with the removal of both dyes	1007:1081	The negative values of ΔH and ΔF accompanying with the removal of both dyes specified the adsorption process to be exothermic and spontaneous.					
32512098	9	1	theme	non-toxicity	1311:1322	arg1	benefits					1281:1288	The combined benefits	1268:1288	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability	1268:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	8	2	theme	ad-/de-sorption	1244:1258	arg1	cycles					1260:1265	three consecutive ad-/de-sorption cycles	1226:1265	three consecutive ad-/de-sorption cycles	1226:1265	Regeneration study showed that MChs/Ppy could be reused efficaciously up to three consecutive ad-/de-sorption cycles.					
32512098	6	3	theme	maximum	918:924	arg1	capacity					945:952	the maximum monolayer sorption capacity	914:952	the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively	914:1004	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	2	4	theme	XRD	465:467	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	7	5	theme	ΔF	1037:1038	arg1	values					1020:1025	The negative values	1007:1025	The negative values of ΔH and ΔF accompanying with the removal of both dyes	1007:1081	The negative values of ΔH and ΔF accompanying with the removal of both dyes specified the adsorption process to be exothermic and spontaneous.					
32512098	9	6	theme	adsorption	1334:1343	arg1	potential					1345:1353	good adsorption potential	1329:1353	good adsorption potential	1329:1353	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	7	7	theme	adsorption	1097:1106	arg1	process					1108:1114	the adsorption process	1093:1114	the adsorption process	1093:1114	The negative values of ΔH and ΔF accompanying with the removal of both dyes specified the adsorption process to be exothermic and spontaneous.					
32512098	6	8	theme	equilibrium	823:833	arg1	data					835:838	The adsorption equilibrium data	808:838	The adsorption equilibrium data for both the dyes	808:856	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	3	9	theme	MChs/Ppy	498:505	arg1	performance					483:493	The performance	479:493	The performance of MChs/Ppy towards the adsorption of CV and MO	479:541	The performance of MChs/Ppy towards the adsorption of CV and MO was investigated by the batch method.					
32512098	9	10	theme	potential	1345:1353	arg1	benefits					1281:1288	The combined benefits	1268:1288	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability	1268:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	6	11	theme	adsorption	812:821	arg1	data					835:838	The adsorption equilibrium data	808:838	The adsorption equilibrium data for both the dyes	808:856	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	3	12	theme	CV	533:534	arg1	adsorption					519:528	the adsorption	515:528	the adsorption of CV and MO	515:541	The performance of MChs/Ppy towards the adsorption of CV and MO was investigated by the batch method.					
32512098	1	13	theme	cationic	292:299	arg1	violet					309:314	cationic crystal violet and anionic methyl orange dye	292:344	violet	309:314	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	6	14	theme	89.29 mg/g	967:976	arg1	capacity					945:952	the maximum monolayer sorption capacity	914:952	the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively	914:1004	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	9	15	theme	anionic	1376:1382	arg1	dyes					1384:1387	cationic and anionic dyes	1363:1387	cationic and anionic dyes along with easy magnetic separability	1363:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	2	16	theme	TGA	425:427	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	5	17	theme	CV	696:697	arg1	adsorption					682:691	The adsorption	678:691	The adsorption of CV	678:697	The adsorption of CV was well described by pseudo 2nd order, while that of MO was best followed by the pseudo 1st order kinetics.					
32512098	5	18	theme	pseudo	721:726	arg1	order					732:736	pseudo 2nd order	721:736	pseudo 2nd order	721:736	The adsorption of CV was well described by pseudo 2nd order, while that of MO was best followed by the pseudo 1st order kinetics.					
32512098	7	19	theme	dyes	1078:1081	arg1	removal					1062:1068	the removal	1058:1068	the removal of both dyes	1058:1081	The negative values of ΔH and ΔF accompanying with the removal of both dyes specified the adsorption process to be exothermic and spontaneous.					
32512098	1	20	theme	adsorption	181:190	arg1	characteristic					192:205	The adsorption characteristic	177:205	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater	177:360	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	2	21	theme	FTIR	450:453	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	9	22	theme	combined	1272:1279	arg1	benefits					1281:1288	The combined benefits	1268:1288	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability	1268:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	6	23	theme	adsorption	889:898	arg1	isotherm					900:907	Langmuir adsorption isotherm	880:907	Langmuir adsorption isotherm	880:907	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	5	24	theme	1st	788:790	arg1	kinetics					798:805	the pseudo 1st order kinetics	777:805	the pseudo 1st order kinetics	777:805	The adsorption of CV was well described by pseudo 2nd order, while that of MO was best followed by the pseudo 1st order kinetics.					
32512098	0	25	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of polypyrrole	0:30	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	1	26	theme	crystal	301:307	arg1	violet					309:314	cationic crystal violet and anionic methyl orange dye	292:344	violet	309:314	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	9	27	theme	facile	1293:1298	arg1	synthesis					1300:1308	facile synthesis	1293:1308	facile synthesis	1293:1308	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	5	28	theme	order	792:796	arg1	kinetics					798:805	the pseudo 1st order kinetics	777:805	the pseudo 1st order kinetics	777:805	The adsorption of CV was well described by pseudo 2nd order, while that of MO was best followed by the pseudo 1st order kinetics.					
32512098	0	29	theme	polypyrrole	20:30	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of polypyrrole	0:30	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	1	30	theme	violet	309:314	arg1	elimination					277:287	the elimination	273:287	the elimination of cationic crystal violet and anionic methyl orange dye from wastewater	273:360	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	9	31	theme	cationic	1363:1370	arg1	dyes					1384:1387	cationic and anionic dyes	1363:1387	cationic and anionic dyes along with easy magnetic separability	1363:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	8	32	theme	consecutive	1232:1242	arg1	cycles					1260:1265	three consecutive ad-/de-sorption cycles	1226:1265	three consecutive ad-/de-sorption cycles	1226:1265	Regeneration study showed that MChs/Ppy could be reused efficaciously up to three consecutive ad-/de-sorption cycles.					
32512098	1	33	theme	polypyrrole	210:220	arg1	MChs/Ppy					259:266	MChs/Ppy	259:266	MChs/Ppy	259:266	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	1	33	theme	polypyrrole	210:220	arg1	magsorbent					247:256	polypyrrole decorated chitosan-based magsorbent	210:256	polypyrrole decorated chitosan-based magsorbent (MChs/Ppy)	210:267	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	0	34	theme	cationic	130:137	arg1	dyes					151:154	cationic and anionic dyes	130:154	cationic and anionic dyes	130:154	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	6	35	dep	dyes	853:856	arg1	both					844:847	both	844:847	both	844:847	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	3	36	theme	MO	540:541	arg1	adsorption					519:528	the adsorption	515:528	the adsorption of CV and MO	515:541	The performance of MChs/Ppy towards the adsorption of CV and MO was investigated by the batch method.					
32512098	1	37	theme	decorated	222:230	arg1	MChs/Ppy					259:266	MChs/Ppy	259:266	MChs/Ppy	259:266	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	1	37	theme	decorated	222:230	arg1	magsorbent					247:256	polypyrrole decorated chitosan-based magsorbent	210:256	polypyrrole decorated chitosan-based magsorbent (MChs/Ppy)	210:267	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	0	38	theme	chitosan-based	42:55	arg1	Characterizations					69:85	chitosan-based magsorbent: Characterizations	42:85	chitosan-based magsorbent: Characterizations	42:85	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	1	39	theme	anionic	320:326	arg1	dye					342:344	cationic crystal violet and anionic methyl orange dye	292:344	dye	342:344	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	2	40	theme	SEM/EDAX	430:437	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	3	41	theme	batch	567:571	arg1	method					573:578	the batch method	563:578	the batch method	563:578	The performance of MChs/Ppy towards the adsorption of CV and MO was investigated by the batch method.					
32512098	8	42	theme	Regeneration	1150:1161	arg1	study					1163:1167	Regeneration study	1150:1167	Regeneration study	1150:1167	Regeneration study showed that MChs/Ppy could be reused efficaciously up to three consecutive ad-/de-sorption cycles.					
32512098	1	43	theme	chitosan-based	232:245	arg1	MChs/Ppy					259:266	MChs/Ppy	259:266	MChs/Ppy	259:266	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	1	43	theme	chitosan-based	232:245	arg1	magsorbent					247:256	polypyrrole decorated chitosan-based magsorbent	210:256	polypyrrole decorated chitosan-based magsorbent (MChs/Ppy)	210:267	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	5	44	theme	2nd	728:730	arg1	order					732:736	pseudo 2nd order	721:736	pseudo 2nd order	721:736	The adsorption of CV was well described by pseudo 2nd order, while that of MO was best followed by the pseudo 1st order kinetics.					
32512098	1	45	theme	methyl	328:333	arg1	dye					342:344	cationic crystal violet and anionic methyl orange dye	292:344	dye	342:344	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	0	46	theme	anionic	143:149	arg1	dyes					151:154	cationic and anionic dyes	130:154	cationic and anionic dyes	130:154	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	2	47	theme	BET	445:447	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	9	48	theme	good	1329:1332	arg1	potential					1345:1353	good adsorption potential	1329:1353	good adsorption potential	1329:1353	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	1	49	theme	magsorbent	247:256	arg1	characteristic					192:205	The adsorption characteristic	177:205	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater	177:360	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	2	50	theme	VSM	456:458	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	1	51	theme	orange	335:340	arg1	dye					342:344	cationic crystal violet and anionic methyl orange dye	292:344	dye	342:344	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	0	52	theme	aqueous	161:167	arg1	medium					169:174	aqueous medium	161:174	aqueous medium	161:174	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	6	53	theme	Langmuir	880:887	arg1	adsorption					889:898	Langmuir adsorption	880:898	Langmuir adsorption isotherm	880:907	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	9	54	theme	easy	1400:1403	arg1	separability					1414:1425	easy magnetic separability	1400:1425	cationic and anionic dyes along with easy magnetic separability	1363:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	4	55	theme	optimum	658:664	arg1	conditions					666:675	the optimum conditions	654:675	the optimum conditions	654:675	Removal performance was obtained to be 88.11 and 92.89% for CV and MO at the optimum conditions.					
32512098	0	56	theme	magsorbent	57:66	arg1	Characterizations					69:85	chitosan-based magsorbent: Characterizations	42:85	chitosan-based magsorbent: Characterizations	42:85	Facile synthesis of polypyrrole decorated chitosan-based magsorbent: Characterizations, performance, and applications in removing cationic and anionic dyes from aqueous medium.					
32512098	1	57	theme	dye	342:344	arg1	elimination					277:287	the elimination	273:287	the elimination of cationic crystal violet and anionic methyl orange dye from wastewater	273:360	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	7	58	theme	negative	1011:1018	arg1	values					1020:1025	The negative values	1007:1025	The negative values of ΔH and ΔF accompanying with the removal of both dyes	1007:1081	The negative values of ΔH and ΔF accompanying with the removal of both dyes specified the adsorption process to be exothermic and spontaneous.					
32512098	9	59	theme	synthesis	1300:1308	arg1	benefits					1281:1288	The combined benefits	1268:1288	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability	1268:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	4	60	from	conditions	666:675	arg1	%					635:635	88.11 and 92.89%	620:635	88.11 and 92.89% for CV and MO	620:649	Removal performance was obtained to be 88.11 and 92.89% for CV and MO at the optimum conditions.					
32512098	4	61	theme	Removal	581:587	arg1	performance					589:599	Removal performance	581:599	Removal performance	581:599	Removal performance was obtained to be 88.11 and 92.89% for CV and MO at the optimum conditions.					
32512098	8	62	dep	cycles	1260:1265	arg1	up					1220:1221	up	1220:1221	up	1220:1221	Regeneration study showed that MChs/Ppy could be reused efficaciously up to three consecutive ad-/de-sorption cycles.					
32512098	9	63	theme	magnetic	1405:1412	arg1	separability					1414:1425	easy magnetic separability	1400:1425	cationic and anionic dyes along with easy magnetic separability	1363:1425	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	6	64	theme	62.89	957:961	arg1	capacity					945:952	the maximum monolayer sorption capacity	914:952	the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively	914:1004	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	9	65	dep	along	1389:1393	arg1	with					1395:1398	with	1395:1398	with	1395:1398	The combined benefits of facile synthesis, non-toxicity, and good adsorption potential towards cationic and anionic dyes along with easy magnetic separability make MChs/Ppy as a novel adsorbent.					
32512098	2	66	theme	TEM	440:442	arg1	analyses					469:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses	425:476	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	2	67	theme	MChs/Ppy	400:407	arg1	characteristics					381:395	The characteristics	377:395	The characteristics of MChs/Ppy	377:407	The characteristics of MChs/Ppy were studied by TGA, SEM/EDAX, TEM, BET, FTIR, VSM, and XRD analyses.					
32512098	5	68	theme	pseudo	781:786	arg1	kinetics					798:805	the pseudo 1st order kinetics	777:805	the pseudo 1st order kinetics	777:805	The adsorption of CV was well described by pseudo 2nd order, while that of MO was best followed by the pseudo 1st order kinetics.					
32512098	6	69	theme	sorption	936:943	arg1	capacity					945:952	the maximum monolayer sorption capacity	914:952	the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively	914:1004	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32512098	1	70	from	wastewater	351:360	arg1	elimination					277:287	the elimination	273:287	the elimination of cationic crystal violet and anionic methyl orange dye from wastewater	273:360	The adsorption characteristic of polypyrrole decorated chitosan-based magsorbent (MChs/Ppy) for the elimination of cationic crystal violet and anionic methyl orange dye from wastewater were explored.					
32512098	6	71	theme	monolayer	926:934	arg1	capacity					945:952	the maximum monolayer sorption capacity	914:952	the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively	914:1004	The adsorption equilibrium data for both the dyes were fitted well with Langmuir adsorption isotherm with the maximum monolayer sorption capacity of 62.89 and 89.29 mg/g for CV and MO, respectively.					
32516705	0	0	theme	agroindustrial	89:102	arg1	wastes					104:109	different agroindustrial wastes	79:109	different agroindustrial wastes	79:109	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	4	1	theme	%	533:533	arg1	residue					541:547	94% grape residue	531:547	94% grape residue	531:547	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	0	2	theme	different	79:87	arg1	wastes					104:109	different agroindustrial wastes	79:109	different agroindustrial wastes	79:109	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	6	3	theme	fibre	746:750	arg1	contents					713:720	The contents	709:720	The contents of proteins and dietary fibre	709:750	The contents of proteins and dietary fibre were 16.59% and 59.61%, respectively.					
32516705	6	3	theme	fibre	746:750	arg1	%					762:762	16.59%	757:762	16.59%	757:762	The contents of proteins and dietary fibre were 16.59% and 59.61%, respectively.					
32516705	7	4	dep	content	849:855	arg1	the					828:830	the	828:830	the	828:830	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	4	5	theme	94	531:532	arg1	%					533:533	%	533:533	%	533:533	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	5	6	theme	highest	672:678	arg1	value					680:684	the highest value	668:684	the highest value (13.14%) of ß-glucans	668:706	Mushrooms grown in this condition showed the highest value (13.14%) of ß-glucans.					
32516705	5	6	theme	highest	672:678	arg1	%					692:692	13.14%	687:692	13.14%	687:692	Mushrooms grown in this condition showed the highest value (13.14%) of ß-glucans.					
32516705	7	7	theme	291.51 ± 1.83 mg	858:873	arg1	content					849:855	phenolic content	840:855	phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom)	840:894	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	7	theme	291.51 ± 1.83 mg	858:873	arg1	mushroom					886:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	3	8	theme	ß-glucans	439:447	arg1	composition					457:467	the composition	453:467	the composition of S. commune	453:481	The content of ß-glucans and the composition of S. commune were also evaluated.					
32516705	3	8	theme	ß-glucans	439:447	arg1	content					428:434	The content	424:434	The content of ß-glucans	424:447	The content of ß-glucans and the composition of S. commune were also evaluated.					
32516705	2	9	dep	substrates	389:398	arg1	the					385:387	the	385:387	the	385:387	The content of phenolics and antioxidant activity were determined for the substrates and mushrooms produced.					
32516705	6	10	theme	proteins	725:732	arg1	contents					713:720	The contents	709:720	The contents of proteins and dietary fibre	709:750	The contents of proteins and dietary fibre were 16.59% and 59.61%, respectively.					
32516705	6	10	theme	proteins	725:732	arg1	%					762:762	16.59%	757:762	16.59%	757:762	The contents of proteins and dietary fibre were 16.59% and 59.61%, respectively.					
32516705	2	11	theme	phenolics	330:338	arg1	content					319:325	The content	315:325	The content of phenolics and antioxidant activity	315:363	The content of phenolics and antioxidant activity were determined for the substrates and mushrooms produced.					
32516705	2	12	theme	antioxidant	344:354	arg1	activity					356:363	antioxidant activity	344:363	antioxidant activity	344:363	The content of phenolics and antioxidant activity were determined for the substrates and mushrooms produced.					
32516705	4	13	theme	grape	535:539	arg1	residue					541:547	94% grape residue	531:547	94% grape residue	531:547	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	1	14	theme	different	195:203	arg1	media					213:217	different culture media	195:217	different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC)	195:312	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	5	15	theme	ß-glucans	698:706	arg1	value					680:684	the highest value	668:684	the highest value (13.14%) of ß-glucans	668:706	Mushrooms grown in this condition showed the highest value (13.14%) of ß-glucans.					
32516705	5	15	theme	ß-glucans	698:706	arg1	%					692:692	13.14%	687:692	13.14%	687:692	Mushrooms grown in this condition showed the highest value (13.14%) of ß-glucans.					
32516705	7	16	theme	phenolic	840:847	arg1	content					849:855	phenolic content	840:855	phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom)	840:894	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	16	theme	phenolic	840:847	arg1	mushroom					886:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	1	17	theme	culture	205:211	arg1	media					213:217	different culture media	195:217	different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC)	195:312	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	0	18	theme	Chemical	0:7	arg1	features					9:16	Chemical features	0:16	Chemical features	0:16	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	7	19	theme	highest	832:838	arg1	content					849:855	phenolic content	840:855	phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom)	840:894	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	19	theme	highest	832:838	arg1	mushroom					886:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	0	20	theme	antioxidant	22:32	arg1	profile					34:40	antioxidant profile	22:40	antioxidant profile	22:40	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	1	21	theme	cotton	269:274	arg1	cake					276:279	cotton cake	269:279	cotton cake (CC)	269:284	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	21	theme	cotton	269:274	arg1	CC					282:283	CC	282:283	CC	282:283	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	0	22	theme	production	139:148	arg1	byproducts					115:124	byproducts	115:124	byproducts of biodiesel production	115:148	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	0	22	theme	production	139:148	arg1	wastes					104:109	different agroindustrial wastes	79:109	different agroindustrial wastes	79:109	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	7	23	theme	GAE/	875:878	arg1	content					849:855	phenolic content	840:855	phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom)	840:894	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	23	theme	GAE/	875:878	arg1	mushroom					886:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	24	theme	100 g	880:884	arg1	content					849:855	phenolic content	840:855	phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom)	840:894	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	24	theme	100 g	880:884	arg1	mushroom					886:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	291.51 ± 1.83 mg GAE/ 100 g mushroom	858:893	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	4	25	theme	yield	579:583	arg1	values					569:574	the highest values	557:574	the highest values of yield, biological efficiency, and productivity	557:624	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	0	26	theme	biodiesel	129:137	arg1	production					139:148	biodiesel production	129:148	biodiesel production	129:148	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	1	27	theme	pine	230:233	arg1	PS					244:245	PS	244:245	PS	244:245	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	27	theme	pine	230:233	arg1	sawdust					235:241	pine sawdust	230:241	pine sawdust (PS)	230:246	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	4	28	theme	productivity	613:624	arg1	values					569:574	the highest values	557:574	the highest values of yield, biological efficiency, and productivity	557:624	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	8	29	theme	substrate	990:998	arg1	composition					1000:1010	substrate composition	990:1010	substrate composition	990:1010	The results obtained indicate that substrate composition affected the production of S. commune and its chemical composition.					
32516705	4	30	theme	biological	586:595	arg1	efficiency					597:606	biological efficiency	586:606	biological efficiency	586:606	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	8	31	theme	chemical	1058:1065	arg1	composition					1067:1077	its chemical composition	1054:1077	its chemical composition	1054:1077	The results obtained indicate that substrate composition affected the production of S. commune and its chemical composition.					
32516705	7	32	theme	DPPH	935:938	arg1	scavenging					942:951	58.15 ± 0.86 DPPH % scavenging	922:951	58.15 ± 0.86 DPPH % scavenging	922:951	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	32	theme	DPPH	935:938	arg1	activity					912:919	antioxidant activity	900:919	antioxidant activity (58.15 ± 0.86 DPPH % scavenging)	900:952	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	2	33	theme	activity	356:363	arg1	content					319:325	The content	315:325	The content of phenolics and antioxidant activity	315:363	The content of phenolics and antioxidant activity were determined for the substrates and mushrooms produced.					
32516705	7	34	theme	%	940:940	arg1	scavenging					942:951	58.15 ± 0.86 DPPH % scavenging	922:951	58.15 ± 0.86 DPPH % scavenging	922:951	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	34	theme	%	940:940	arg1	activity					912:919	antioxidant activity	900:919	antioxidant activity (58.15 ± 0.86 DPPH % scavenging)	900:952	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	0	35	theme	Schizophyllum	45:57	arg1	commune					59:65	Schizophyllum commune	45:65	Schizophyllum commune	45:65	Chemical features and antioxidant profile by Schizophyllum commune produced on different agroindustrial wastes and byproducts of biodiesel production.					
32516705	1	36	theme	jatropha	290:297	arg1	JC					310:311	JC	310:311	JC	310:311	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	36	theme	jatropha	290:297	arg1	cake					304:307	jatropha seed cake	290:307	jatropha seed cake (JC)	290:312	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	8	37	theme	commune	1042:1048	arg1	composition					1067:1077	its chemical composition	1054:1077	its chemical composition	1054:1077	The results obtained indicate that substrate composition affected the production of S. commune and its chemical composition.					
32516705	8	37	theme	commune	1042:1048	arg1	production					1025:1034	the production	1021:1034	the production of S. commune	1021:1048	The results obtained indicate that substrate composition affected the production of S. commune and its chemical composition.					
32516705	8	38	theme	S.	1039:1040	arg1	commune					1042:1048	S. commune	1039:1048	S. commune	1039:1048	The results obtained indicate that substrate composition affected the production of S. commune and its chemical composition.					
32516705	7	39	theme	antioxidant	900:910	arg1	scavenging					942:951	58.15 ± 0.86 DPPH % scavenging	922:951	58.15 ± 0.86 DPPH % scavenging	922:951	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	7	39	theme	antioxidant	900:910	arg1	activity					912:919	antioxidant activity	900:919	antioxidant activity (58.15 ± 0.86 DPPH % scavenging)	900:952	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	1	40	theme	Schizophyllum	151:163	arg1	commune					165:171	Schizophyllum commune VE_07	151:177	Schizophyllum commune VE_07	151:177	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	7	41	theme	cotton	809:814	arg1	cake					816:819	cotton cake	809:819	cotton cake	809:819	Mushrooms grown in cotton cake showed the highest phenolic content (291.51 ± 1.83 mg GAE/ 100 g mushroom) and antioxidant activity (58.15 ± 0.86 DPPH % scavenging).					
32516705	1	42	contain	containing	219:228	arg2	sawdust					235:241	pine sawdust	230:241	pine sawdust (PS)	230:246	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	residue					255:261	grape residue	249:261	grape residue (GR)	249:266	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	cake					304:307	jatropha seed cake	290:307	jatropha seed cake (JC)	290:312	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	cake					276:279	cotton cake	269:279	cotton cake (CC)	269:284	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	GR					264:265	GR	264:265	GR	264:265	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	JC					310:311	JC	310:311	JC	310:311	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	CC					282:283	CC	282:283	CC	282:283	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg1	media					213:217	different culture media	195:217	different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC)	195:312	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	42	contain	containing	219:228	arg2	PS					244:245	PS	244:245	PS	244:245	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	3	43	theme	S.	472:473	arg1	commune					475:481	S. commune	472:481	S. commune	472:481	The content of ß-glucans and the composition of S. commune were also evaluated.					
32516705	4	44	theme	efficiency	597:606	arg1	values					569:574	the highest values	557:574	the highest values of yield, biological efficiency, and productivity	557:624	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	1	45	theme	seed	299:302	arg1	JC					310:311	JC	310:311	JC	310:311	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	45	theme	seed	299:302	arg1	cake					304:307	jatropha seed cake	290:307	jatropha seed cake (JC)	290:312	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	4	46	theme	highest	561:567	arg1	values					569:574	the highest values	557:574	the highest values of yield, biological efficiency, and productivity	557:624	The medium formulated with 94% grape residue enabled the highest values of yield, biological efficiency, and productivity.					
32516705	3	47	theme	commune	475:481	arg1	composition					457:467	the composition	453:467	the composition of S. commune	453:481	The content of ß-glucans and the composition of S. commune were also evaluated.					
32516705	3	47	theme	commune	475:481	arg1	content					428:434	The content	424:434	The content of ß-glucans	424:447	The content of ß-glucans and the composition of S. commune were also evaluated.					
32516705	1	48	theme	grape	249:253	arg1	GR					264:265	GR	264:265	GR	264:265	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
32516705	1	48	theme	grape	249:253	arg1	residue					255:261	grape residue	249:261	grape residue (GR)	249:266	Schizophyllum commune VE_07 was produced in different culture media containing pine sawdust (PS), grape residue (GR), cotton cake (CC) and jatropha seed cake (JC).					
34237070	7	0	theme	ripeness	1290:1297	arg1	stage					1281:1285	stage	1281:1285	stage of ripeness	1281:1297	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	2	1	theme	assessed	705:712	arg1	stages					714:719	the same assessed stages	696:719	the same assessed stages of ripeness	696:731	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	1	2	theme	assessed	371:378	arg1	ripeness					380:387	assessed ripeness	371:387	assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers	371:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	3	theme	mEG	1508:1510	arg1	approach					1512:1519	the mEG approach	1504:1519	the mEG approach	1504:1519	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	5	4	theme	ripe	1031:1034	arg1	bananas					1036:1042	Slightly ripe and ripe bananas	1013:1042	Slightly ripe and ripe bananas	1013:1042	Slightly ripe and ripe bananas differed by ~1.1 g/100g in the controlled single-lot study but not among retail samples.					
34237070	3	5	dep	g/100g	814:819	arg1	affected					829:836	affected	829:836	affected by ripeness	829:848	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	2	6	theme	different	641:649	arg1	stages					651:656	different stages	641:656	different stages of ripeness	641:668	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	1	7	theme	controlled	394:403	arg1	study					405:409	a controlled study	392:409	a controlled study of a single lot of bananas	392:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	4	8	theme	ripe	973:976	arg1	g/100g					985:990	ripe and ~2 g/100g	973:990	ripe and ~2 g/100g in overripe bananas	973:1010	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	0	9	theme	retail	84:89	arg1	market					91:96	the retail market	80:96	the retail market	80:96	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	8	10	theme	carbohydrate	1566:1577	arg1	protocols					1590:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	1	11	theme	dietary	148:154	arg1	fiber					156:160	dietary fiber	148:160	dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch	148:352	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	2	12	from	lot	633:635	arg1	bananas					611:617	bananas	611:617	bananas from a single lot	611:635	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	8	13	theme	food	1604:1607	arg1	data					1621:1624	food composition data	1604:1624	food composition data	1604:1624	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	4	14	from	g/100g	919:924	arg1	fruit					936:940	unripe fruit	929:940	unripe fruit	929:940	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	7	15	theme	post-harvest	1397:1408	arg1	handling					1410:1417	post-harvest handling	1397:1417	post-harvest handling	1397:1417	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	2	16	theme	single	626:631	arg1	lot					633:635	a single lot	624:635	a single lot	624:635	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	5	17	theme	retail	1117:1122	arg1	samples					1124:1130	retail samples	1117:1130	retail samples	1117:1130	Slightly ripe and ripe bananas differed by ~1.1 g/100g in the controlled single-lot study but not among retail samples.					
34237070	4	18	theme	Mean	851:854	arg1	fiber					856:860	Mean fiber	851:860	Mean fiber assessed with the recently modified method (mEG)	851:909	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	5	19	theme	controlled	1075:1084	arg1	study					1097:1101	the controlled single-lot study	1071:1101	the controlled single-lot study	1071:1101	Slightly ripe and ripe bananas differed by ~1.1 g/100g in the controlled single-lot study but not among retail samples.					
34237070	0	20	theme	different	48:56	arg1	stages					58:63	different stages	48:63	different stages of ripeness in the retail market	48:96	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	1	21	theme	work	116:119	arg1	goal					103:106	The goal	99:106	The goal of this work	99:119	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	0	22	from	sugars	27:32	arg1	bananas					37:43	bananas	37:43	bananas	37:43	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	1	23	theme	same	460:463	arg1	stages					474:479	the same assessed stages	456:479	the same assessed stages of ripeness from bananas purchased in retail stores	456:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	24	theme	stages	1667:1672	arg1	impact					1647:1652	the impact	1643:1652	the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data	1643:1757	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	3	25	theme	traditional	761:771	arg1	method					795:800	the traditional enzymatic-gravimetric method	757:800	the traditional enzymatic-gravimetric method (EG)	757:805	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	3	25	theme	traditional	761:771	arg1	EG					803:804	EG	803:804	EG	803:804	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	4	26	theme	modified	889:896	arg1	mEG					906:908	mEG	906:908	mEG	906:908	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	4	26	theme	modified	889:896	arg1	method					898:903	the recently modified method	876:903	the recently modified method (mEG)	876:909	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	6	27	from	increase	1151:1158	arg1	glucose					1173:1179	glucose	1173:1179	glucose	1173:1179	There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe.					
34237070	6	27	from	increase	1151:1158	arg1	sugar					1191:1195	total sugar	1185:1195	total sugar	1185:1195	There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe.					
34237070	6	27	from	increase	1151:1158	arg1	fructose					1163:1170	fructose	1163:1170	fructose	1163:1170	There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe.					
34237070	2	28	theme	dietary	580:586	arg1	fiber					588:592	dietary fiber	580:592	dietary fiber	580:592	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	0	29	theme	Dietary	0:6	arg1	fiber					8:12	Dietary fiber	0:12	Dietary fiber	0:12	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	8	30	theme	maturity	1677:1684	arg1	stages					1667:1672	different stages	1657:1672	different stages of maturity and ripeness	1657:1697	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	2	31	from	stages	714:719	arg1	stages					651:656	different stages	641:656	different stages of ripeness	641:668	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	1	32	theme	ripeness	484:491	arg1	stages					474:479	the same assessed stages	456:479	the same assessed stages of ripeness from bananas purchased in retail stores	456:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	4	33	theme	~2	982:983	arg1	g/100g					985:990	ripe and ~2 g/100g	973:990	ripe and ~2 g/100g in overripe bananas	973:1010	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	7	34	from	differences	1369:1379	arg1	cultivar					1384:1391	cultivar	1384:1391	cultivar	1384:1391	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	7	34	from	differences	1369:1379	arg1	handling					1410:1417	post-harvest handling	1397:1417	post-harvest handling	1397:1417	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	1	35	theme	lot	423:425	arg1	study					405:409	a controlled study	392:409	a controlled study of a single lot of bananas	392:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	36	theme	modified	264:271	arg1	method					295:300	the more recently accepted modified enzymatic-gravimetric method	237:300	the more recently accepted modified enzymatic-gravimetric method	237:300	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	7	37	theme	carbohydrate	1304:1315	arg1	composition					1317:1327	the carbohydrate composition	1300:1327	the carbohydrate composition in retail bananas	1300:1345	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	1	38	theme	bananas	430:436	arg1	bananas					430:436	bananas	430:436	bananas	430:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	38	theme	bananas	430:436	arg1	lot					423:425	a single lot	414:425	a single lot of bananas	414:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	39	from	study	405:409	arg1	changes					137:143	changes	137:143	changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch	137:352	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	39	from	study	405:409	arg1	function					359:366	a function	357:366	a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers	357:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	2	40	theme	retail	678:683	arg1	samples					685:691	retail samples	678:691	retail samples	678:691	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	1	41	theme	traditional	178:188	arg1	AOAC					303:306	AOAC 2011.25	303:314	AOAC 2011.25	303:314	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	41	theme	traditional	178:188	arg1	AOAC					220:223	AOAC 991.43	220:230	AOAC 991.43	220:230	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	41	theme	traditional	178:188	arg1	method					212:217	the traditional enzymatic-gravimetric method	174:217	the traditional enzymatic-gravimetric method (AOAC 991.43)	174:231	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	42	theme	ripeness	1690:1697	arg1	stages					1667:1672	different stages	1657:1672	different stages of maturity and ripeness	1657:1697	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	7	43	from	composition	1317:1327	arg1	bananas					1339:1345	retail bananas	1332:1345	retail bananas	1332:1345	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	1	44	from	ripeness	380:387	arg1	study					405:409	a controlled study	392:409	a controlled study of a single lot of bananas	392:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	44	from	ripeness	380:387	arg1	stages					474:479	the same assessed stages	456:479	the same assessed stages of ripeness from bananas purchased in retail stores	456:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	44	from	ripeness	380:387	arg1	bananas					445:451	bananas	445:451	bananas	445:451	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	44	from	ripeness	380:387	arg1	suppliers					549:557	different suppliers	539:557	different suppliers	539:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	2	45	theme	same	700:703	arg1	stages					714:719	the same assessed stages	696:719	the same assessed stages of ripeness	696:731	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	8	46	theme	carbohydrate	1702:1713	arg1	intake					1715:1720	carbohydrate intake	1702:1720	carbohydrate intake estimated from food composition data	1702:1757	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	1	47	theme	ripeness	380:387	arg1	changes					137:143	changes	137:143	changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch	137:352	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	47	theme	ripeness	380:387	arg1	function					359:366	a function	357:366	a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers	357:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	48	from	suppliers	549:557	arg1	bananas					445:451	bananas	445:451	bananas	445:451	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	48	from	suppliers	549:557	arg1	ripeness					380:387	assessed ripeness	371:387	assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers	371:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	6	49	theme	total	1185:1189	arg1	sugar					1191:1195	total sugar	1185:1195	total sugar	1185:1195	There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe.					
34237070	8	50	theme	food	1737:1740	arg1	data					1754:1757	food composition data	1737:1757	food composition data	1737:1757	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	8	51	theme	comprehensive	1538:1550	arg1	protocols					1590:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	0	52	from	stages	58:63	arg1	starch					15:20	starch	15:20	starch	15:20	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	0	52	from	stages	58:63	arg1	sugars					27:32	sugars	27:32	sugars	27:32	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	0	52	from	stages	58:63	arg1	fiber					8:12	Dietary fiber	0:12	Dietary fiber	0:12	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	0	52	from	stages	58:63	arg1	market					91:96	the retail market	80:96	the retail market	80:96	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	2	53	theme	ripeness	661:668	arg1	stages					651:656	different stages	641:656	different stages of ripeness	641:668	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	6	54	theme	large	1145:1149	arg1	increase					1151:1158	a large increase	1143:1158	a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe	1143:1267	There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe.					
34237070	5	55	theme	single-lot	1086:1095	arg1	study					1097:1101	the controlled single-lot study	1071:1101	the controlled single-lot study	1071:1101	Slightly ripe and ripe bananas differed by ~1.1 g/100g in the controlled single-lot study but not among retail samples.					
34237070	8	56	theme	sensitive	1556:1564	arg1	protocols					1590:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	1	57	from	bananas	498:504	arg1	ripeness					484:491	ripeness	484:491	ripeness from bananas purchased in retail stores	484:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	57	from	bananas	498:504	arg1	stages					474:479	the same assessed stages	456:479	the same assessed stages of ripeness from bananas purchased in retail stores	456:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	58	theme	analytical	1579:1588	arg1	protocols					1590:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	more comprehensive and sensitive carbohydrate analytical protocols	1533:1598	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	8	59	from	impact	1647:1652	arg1	intake					1715:1720	carbohydrate intake	1702:1720	carbohydrate intake estimated from food composition data	1702:1757	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	8	60	from	study	1438:1442	arg1	Results					1420:1426	Results	1420:1426	Results from this study	1420:1442	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	3	61	theme	Mean	734:737	arg1	fiber					739:743	Mean fiber	734:743	Mean fiber measured by the traditional enzymatic-gravimetric method (EG)	734:805	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	0	62	from	ripeness	68:75	arg1	market					91:96	the retail market	80:96	the retail market	80:96	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	1	63	from	stages	474:479	arg1	bananas					445:451	bananas	445:451	bananas	445:451	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	63	from	stages	474:479	arg1	ripeness					380:387	assessed ripeness	371:387	assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers	371:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	63	from	stages	474:479	arg1	bananas					498:504	bananas	498:504	bananas purchased in retail stores	498:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	64	theme	retail	519:524	arg1	stores					526:531	retail stores	519:531	retail stores	519:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	65	from	function	359:366	arg1	study					405:409	a controlled study	392:409	a controlled study of a single lot of bananas	392:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	65	from	function	359:366	arg1	bananas					445:451	bananas	445:451	bananas	445:451	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	66	theme	composition	1609:1619	arg1	data					1621:1624	food composition data	1604:1624	food composition data	1604:1624	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	0	67	from	starch	15:20	arg1	bananas					37:43	bananas	37:43	bananas	37:43	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	4	68	theme	overripe	995:1002	arg1	bananas					1004:1010	overripe bananas	995:1010	overripe bananas	995:1010	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	8	69	theme	composition	1742:1752	arg1	data					1754:1757	food composition data	1737:1757	food composition data	1737:1757	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	0	70	from	fiber	8:12	arg1	bananas					37:43	bananas	37:43	bananas	37:43	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	1	71	from	changes	137:143	arg1	fiber					156:160	dietary fiber	148:160	dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch	148:352	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	72	theme	different	539:547	arg1	suppliers					549:557	different suppliers	539:557	different suppliers	539:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	0	73	theme	ripeness	68:75	arg1	stages					58:63	different stages	48:63	different stages of ripeness in the retail market	48:96	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	1	74	from	bananas	445:451	arg1	changes					137:143	changes	137:143	changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch	137:352	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	74	from	bananas	445:451	arg1	function					359:366	a function	357:366	a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers	357:557	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	75	theme	assessed	465:472	arg1	stages					474:479	the same assessed stages	456:479	the same assessed stages of ripeness from bananas purchased in retail stores	456:531	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	76	theme	different	1657:1665	arg1	stages					1667:1672	different stages	1657:1672	different stages of maturity and ripeness	1657:1697	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	3	77	theme	enzymatic-gravimetric	773:793	arg1	method					795:800	the traditional enzymatic-gravimetric method	757:800	the traditional enzymatic-gravimetric method (EG)	757:805	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	3	77	theme	enzymatic-gravimetric	773:793	arg1	EG					803:804	EG	803:804	EG	803:804	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	6	78	with	ripe	1218:1221	arg1	differences					1231:1241	no differences	1228:1241	no differences between ripe and overripe	1228:1267	There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe.					
34237070	3	79	theme	~2	811:812	arg1	g/100g					814:819	g/100g	814:819	g/100g	814:819	Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness.					
34237070	5	80	theme	ripe	1022:1025	arg1	bananas					1036:1042	Slightly ripe and ripe bananas	1013:1042	Slightly ripe and ripe bananas	1013:1042	Slightly ripe and ripe bananas differed by ~1.1 g/100g in the controlled single-lot study but not among retail samples.					
34237070	0	81	from	market	91:96	arg1	stages					58:63	different stages	48:63	different stages of ripeness in the retail market	48:96	Dietary fiber, starch, and sugars in bananas at different stages of ripeness in the retail market.					
34237070	1	82	theme	single	416:421	arg1	bananas					430:436	bananas	430:436	bananas	430:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	82	theme	single	416:421	arg1	lot					423:425	a single lot	414:425	a single lot of bananas	414:436	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	83	theme	accepted	255:262	arg1	method					295:300	the more recently accepted modified enzymatic-gravimetric method	237:300	the more recently accepted modified enzymatic-gravimetric method	237:300	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	7	84	theme	retail	1332:1337	arg1	bananas					1339:1345	retail bananas	1332:1345	retail bananas	1332:1345	Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling.					
34237070	1	85	theme	enzymatic-gravimetric	273:293	arg1	method					295:300	the more recently accepted modified enzymatic-gravimetric method	237:300	the more recently accepted modified enzymatic-gravimetric method	237:300	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	8	86	theme	dietary	1484:1490	arg1	fiber					1492:1496	dietary fiber	1484:1496	dietary fiber using the mEG approach	1484:1519	Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.					
34237070	4	87	from	g/100g	985:990	arg1	bananas					1004:1010	overripe bananas	995:1010	overripe bananas	995:1010	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	4	88	theme	unripe	929:934	arg1	fruit					936:940	unripe fruit	929:940	unripe fruit	929:940	Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas.					
34237070	2	89	theme	ripeness	724:731	arg1	stages					714:719	the same assessed stages	696:719	the same assessed stages of ripeness	696:731	Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness.					
34237070	1	90	theme	enzymatic-gravimetric	190:210	arg1	AOAC					303:306	AOAC 2011.25	303:314	AOAC 2011.25	303:314	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	90	theme	enzymatic-gravimetric	190:210	arg1	AOAC					220:223	AOAC 991.43	220:230	AOAC 991.43	220:230	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34237070	1	90	theme	enzymatic-gravimetric	190:210	arg1	method					212:217	the traditional enzymatic-gravimetric method	174:217	the traditional enzymatic-gravimetric method (AOAC 991.43)	174:231	The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers.					
34450144	0	0	theme	dye	82:84	arg1	optimization					117:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	3	1	theme	optimum	484:490	arg1	parameters					507:516	The optimum adsorption key parameters	480:516	The optimum adsorption key parameters	480:516	The optimum adsorption key parameters and their significant interactions were determined by Box-Behnken Design (BBD).					
34450144	7	2	theme	multilayer	1144:1153	arg1	process					1166:1172	a multilayer adsorption process	1142:1172	a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C	1142:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	4	3	with	dose	706:709	arg1	dose					745:748	m-Cs-PVA/FA dose	733:748	m-Cs-PVA/FA dose	733:748	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	4	3	with	dose	706:709	arg1	solution					716:723	solution	716:723	solution pH	716:726	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	1	4	theme	chitosan-polyvinyl	164:181	arg1	m-Cs-PVA/FA					200:210	m-Cs-PVA/FA	200:210	m-Cs-PVA/FA	200:210	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	4	theme	chitosan-polyvinyl	164:181	arg1	ash					195:197	chitosan-polyvinyl alcohol/fly ash	164:197	chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA)	164:211	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	7	5	theme	isotherm	1123:1130	arg1	results					1101:1107	The results	1097:1107	The results of adsorption isotherm	1097:1130	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	3	6	theme	significant	528:538	arg1	interactions					540:551	their significant interactions	522:551	their significant interactions	522:551	The optimum adsorption key parameters and their significant interactions were determined by Box-Behnken Design (BBD).					
34450144	0	7	theme	removal	86:92	arg1	optimization					117:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	8	8	theme	external	1272:1279	arg1	field					1290:1294	An external magnetic field	1269:1294	An external magnetic field	1269:1294	An external magnetic field can be easily applied to recover the adsorbent (m-Cs-PVA/FA).					
34450144	9	9	theme	synthesized	1389:1399	arg1	m-Cs-PVA/FA					1401:1411	the synthesized m-Cs-PVA/FA	1385:1411	the synthesized m-Cs-PVA/FA	1385:1411	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	6	10	theme	RO16	1026:1029	arg1	adsorption					1031:1040	the RO16 adsorption	1022:1040	the RO16 adsorption	1022:1040	The results of adsorption kinetics revealed that the RO16 adsorption was better described by the pseudo-second-order model.					
34450144	0	11	theme	parametric	106:115	arg1	optimization					117:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	4	12	theme	variance	614:621	arg1	analysis					602:609	The analysis	598:609	The analysis of variance (ANOVA)	598:629	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	9	13	theme	preferable	1490:1499	arg1	capacity					1512:1519	preferable adsorption capacity	1490:1519	preferable adsorption capacity	1490:1519	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	1	14	theme	alcohol/fly	183:193	arg1	m-Cs-PVA/FA					200:210	m-Cs-PVA/FA	200:210	m-Cs-PVA/FA	200:210	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	14	theme	alcohol/fly	183:193	arg1	ash					195:197	chitosan-polyvinyl alcohol/fly ash	164:197	chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA)	164:211	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	0	15	theme	Adsorption	95:104	arg1	optimization					117:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	5	16	theme	m-Cs-PVA/FA	871:881	arg1	0.06 g					889:894	0.06 g	889:894	0.06 g	889:894	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	16	theme	m-Cs-PVA/FA	871:881	arg1	dose					883:886	m-Cs-PVA/FA dose	871:886	m-Cs-PVA/FA dose (0.06 g)	871:895	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	7	17	with	model	1206:1210	arg1	capacity					1236:1243	maximum adsorption capacity	1217:1243	maximum adsorption capacity of 123.8 mg/g at 30 °C	1217:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	7	18	theme	123.8 mg/g	1248:1257	arg1	capacity					1236:1243	maximum adsorption capacity	1217:1243	maximum adsorption capacity of 123.8 mg/g at 30 °C	1217:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	4	19	theme	working	755:761	arg1	temperature					763:773	working temperature	755:773	working temperature	755:773	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	6	20	theme	kinetics	999:1006	arg1	results					977:983	The results	973:983	The results of adsorption kinetics	973:1006	The results of adsorption kinetics revealed that the RO16 adsorption was better described by the pseudo-second-order model.					
34450144	1	21	theme	ash	195:197	arg1	blend					155:159	A magnetic biocomposite blend	131:159	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA)	131:211	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	6	22	theme	pseudo-second-order	1070:1088	arg1	model					1090:1094	the pseudo-second-order model	1066:1094	the pseudo-second-order model	1066:1094	The results of adsorption kinetics revealed that the RO16 adsorption was better described by the pseudo-second-order model.					
34450144	6	23	theme	adsorption	988:997	arg1	kinetics					999:1006	adsorption kinetics	988:1006	adsorption kinetics	988:1006	The results of adsorption kinetics revealed that the RO16 adsorption was better described by the pseudo-second-order model.					
34450144	7	24	theme	adsorption	1155:1164	arg1	process					1166:1172	a multilayer adsorption process	1142:1172	a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C	1142:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	4	25	dep	solution	716:723	arg1	pH					725:726	pH	725:726	solution pH	716:726	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	0	26	theme	Fly	0:2	arg1	ash					4:6	Fly ash	0:6	Fly ash	0:6	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	3	27	theme	Box-Behnken	572:582	arg1	BBD					592:594	BBD	592:594	BBD	592:594	The optimum adsorption key parameters and their significant interactions were determined by Box-Behnken Design (BBD).					
34450144	3	27	theme	Box-Behnken	572:582	arg1	Design					584:589	Box-Behnken Design	572:589	Box-Behnken Design (BBD)	572:595	The optimum adsorption key parameters and their significant interactions were determined by Box-Behnken Design (BBD).					
34450144	4	28	theme	significant	645:655	arg1	interactions					657:668	the significant interactions	641:668	the significant interactions	641:668	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	0	29	theme	magnetic	17:24	arg1	blend					53:57	magnetic chitosan-polyvinyl alcohol blend	17:57	magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization	17:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	1	30	theme	polymeric	274:282	arg1	matrix					284:289	the polymeric matrix	270:289	the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA)	270:339	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	4	31	theme	m-Cs-PVA/FA	733:743	arg1	dose					745:748	m-Cs-PVA/FA dose	733:748	m-Cs-PVA/FA dose	733:748	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	2	32	theme	reactive	414:421	arg1	orange					423:428	reactive orange 16	414:431	reactive orange 16	414:431	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	2	32	theme	reactive	414:421	arg1	RO16					434:437	RO16	434:437	RO16	434:437	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	5	33	theme	solution	898:905	arg1	pH					907:908	solution pH	898:908	solution pH (4)	898:912	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	33	theme	solution	898:905	arg1	4					911:911	4	911:911	4	911:911	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	3	34	theme	adsorption	492:501	arg1	parameters					507:516	The optimum adsorption key parameters	480:516	The optimum adsorption key parameters	480:516	The optimum adsorption key parameters and their significant interactions were determined by Box-Behnken Design (BBD).					
34450144	9	35	theme	textile	1473:1479	arg1	dye					1481:1483	textile dye	1473:1483	textile dye with preferable adsorption capacity and separation ability during and after the adsorption process	1473:1582	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	3	36	theme	key	503:505	arg1	parameters					507:516	The optimum adsorption key parameters	480:516	The optimum adsorption key parameters	480:516	The optimum adsorption key parameters and their significant interactions were determined by Box-Behnken Design (BBD).					
34450144	0	37	theme	alcohol	45:51	arg1	blend					53:57	magnetic chitosan-polyvinyl alcohol blend	17:57	magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization	17:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	4	38	with	dose	745:748	arg1	temperature					763:773	working temperature	755:773	working temperature	755:773	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	7	39	theme	adsorption	1225:1234	arg1	capacity					1236:1243	maximum adsorption capacity	1217:1243	maximum adsorption capacity of 123.8 mg/g at 30 °C	1217:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	2	40	dep	dye	409:411	arg1	orange					423:428	reactive orange 16	414:431	reactive orange 16	414:431	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	2	40	dep	dye	409:411	arg1	RO16					434:437	RO16	434:437	RO16	434:437	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	5	41	theme	RO16	843:846	arg1	removal					832:838	the highest removal	820:838	the highest removal of RO16 (%)	820:850	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	0	42	theme	chitosan-polyvinyl	26:43	arg1	blend					53:57	magnetic chitosan-polyvinyl alcohol blend	17:57	magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization	17:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	1	43	theme	magnetic	294:301	arg1	m-Cs-PVA					331:338	m-Cs-PVA	331:338	m-Cs-PVA	331:338	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	43	theme	magnetic	294:301	arg1	alcohol					322:328	magnetic chitosan-polyvinyl alcohol	294:328	magnetic chitosan-polyvinyl alcohol (m-Cs-PVA)	294:339	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	2	44	theme	aquatic	445:451	arg1	environment					453:463	aquatic environment	445:463	aquatic environment	445:463	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	5	45	located	found	856:860	arg1	30 °C					936:940	30 °C	936:940	30 °C	936:940	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	0.06 g					889:894	0.06 g	889:894	0.06 g	889:894	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	17.5 min					962:969	17.5 min	962:969	17.5 min	962:969	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	4					911:911	4	911:911	4	911:911	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	time					956:959	contact time	948:959	contact time (17.5 min)	948:970	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	pH					907:908	solution pH	898:908	solution pH (4)	898:912	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg2	removal					832:838	the highest removal	820:838	the highest removal of RO16 (%)	820:850	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	temperature					923:933	working temperature	915:933	working temperature (30 °C)	915:941	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	45	located	found	856:860	arg1	dose					883:886	m-Cs-PVA/FA dose	871:886	m-Cs-PVA/FA dose (0.06 g)	871:895	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	2	46	theme	textile	401:407	arg1	dye					409:411	textile dye	401:411	textile dye (reactive orange 16, RO16)	401:438	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	9	47	with	dye	1481:1483	arg1	capacity					1512:1519	preferable adsorption capacity	1490:1519	preferable adsorption capacity	1490:1519	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	9	47	with	dye	1481:1483	arg1	ability					1536:1542	separation ability	1525:1542	separation ability	1525:1542	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	1	48	theme	chitosan-polyvinyl	303:320	arg1	m-Cs-PVA					331:338	m-Cs-PVA	331:338	m-Cs-PVA	331:338	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	48	theme	chitosan-polyvinyl	303:320	arg1	alcohol					322:328	magnetic chitosan-polyvinyl alcohol	294:328	magnetic chitosan-polyvinyl alcohol (m-Cs-PVA)	294:339	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	5	49	theme	contact	948:954	arg1	17.5 min					962:969	17.5 min	962:969	17.5 min	962:969	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	49	theme	contact	948:954	arg1	time					956:959	contact time	948:959	contact time (17.5 min)	948:970	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	7	50	from	capacity	1236:1243	arg1	30 °C					1262:1266	30 °C	1262:1266	30 °C	1262:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	5	51	theme	significant	794:804	arg1	interactions					806:817	these significant interactions	788:817	these significant interactions	788:817	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	1	52	theme	alcohol	322:328	arg1	matrix					284:289	the polymeric matrix	270:289	the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA)	270:339	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	7	53	theme	adsorption	1112:1121	arg1	isotherm					1123:1130	adsorption isotherm	1112:1130	adsorption isotherm	1112:1130	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	4	54	theme	m-Cs-PVA/FA	694:704	arg1	dose					706:709	m-Cs-PVA/FA dose	694:709	m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature	694:773	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	9	55	theme	adsorption	1501:1510	arg1	capacity					1512:1519	preferable adsorption capacity	1490:1519	preferable adsorption capacity	1490:1519	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	2	56	attach	remove	394:399	arg2	m-Cs-PVA/FA					363:373	m-Cs-PVA/FA	363:373	m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment	363:463	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	2	56	attach	remove	394:399	arg1	environment					453:463	aquatic environment	445:463	aquatic environment	445:463	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34450144	9	57	theme	adsorption	1565:1574	arg1	process					1576:1582	the adsorption process	1561:1582	the adsorption process	1561:1582	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	7	58	theme	Freundlich	1195:1204	arg1	model					1206:1210	Freundlich model	1195:1210	Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C	1195:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	9	59	theme	separation	1525:1534	arg1	ability					1536:1542	separation ability	1525:1542	separation ability	1525:1542	The results supported that the synthesized m-Cs-PVA/FA presents itself as an effective and promising adsorbent for textile dye with preferable adsorption capacity and separation ability during and after the adsorption process.					
34450144	5	60	theme	working	915:921	arg1	30 °C					936:940	30 °C	936:940	30 °C	936:940	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	5	60	theme	working	915:921	arg1	temperature					923:933	working temperature	915:933	working temperature (30 °C)	915:941	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	7	61	theme	maximum	1217:1223	arg1	capacity					1236:1243	maximum adsorption capacity	1217:1243	maximum adsorption capacity of 123.8 mg/g at 30 °C	1217:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	8	62	theme	magnetic	1281:1288	arg1	field					1290:1294	An external magnetic field	1269:1294	An external magnetic field	1269:1294	An external magnetic field can be easily applied to recover the adsorbent (m-Cs-PVA/FA).					
34450144	1	63	theme	magnetic	133:140	arg1	blend					155:159	A magnetic biocomposite blend	131:159	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA)	131:211	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	64	theme	fly	237:239	arg1	ash					241:243	fly ash	237:243	fly ash (FA) microparticles	237:263	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	64	theme	fly	237:239	arg1	FA					246:247	FA	246:247	FA	246:247	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	0	65	theme	orange	72:77	arg1	optimization					117:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	7	66	from	30 °C	1262:1266	arg1	123.8 mg/g					1248:1257	123.8 mg/g	1248:1257	123.8 mg/g at 30 °C	1248:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	7	66	from	30 °C	1262:1266	arg1	capacity					1236:1243	maximum adsorption capacity	1217:1243	maximum adsorption capacity of 123.8 mg/g at 30 °C	1217:1266	The results of adsorption isotherm indicated a multilayer adsorption process as well described by Freundlich model with maximum adsorption capacity of 123.8 mg/g at 30 °C.					
34450144	1	67	theme	biocomposite	142:153	arg1	blend					155:159	A magnetic biocomposite blend	131:159	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA)	131:211	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	1	68	theme	ash	241:243	arg1	microparticles					250:263	fly ash (FA) microparticles	237:263	fly ash (FA) microparticles	237:263	A magnetic biocomposite blend of chitosan-polyvinyl alcohol/fly ash (m-Cs-PVA/FA) was developed by adding fly ash (FA) microparticles into the polymeric matrix of magnetic chitosan-polyvinyl alcohol (m-Cs-PVA).					
34450144	0	69	theme	reactive	63:70	arg1	optimization					117:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	reactive orange 16 dye removal: Adsorption parametric optimization	63:128	Fly ash modified magnetic chitosan-polyvinyl alcohol blend for reactive orange 16 dye removal: Adsorption parametric optimization.					
34450144	4	70	with	solution	716:723	arg1	temperature					763:773	working temperature	755:773	working temperature	755:773	The analysis of variance (ANOVA) indicates the significant interactions can be observed between m-Cs-PVA/FA dose with solution pH, and m-Cs-PVA/FA dose with working temperature.					
34450144	5	71	theme	highest	824:830	arg1	removal					832:838	the highest removal	820:838	the highest removal of RO16 (%)	820:850	Considering these significant interactions, the highest removal of RO16 (%) was found 90.3% at m-Cs-PVA/FA dose (0.06 g), solution pH (4), working temperature (30 °C), and contact time (17.5 min).					
34450144	2	72	theme	m-Cs-PVA/FA	363:373	arg1	effectiveness					346:358	The effectiveness	342:358	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment	342:463	The effectiveness of m-Cs-PVA/FA as an adsorbent to remove textile dye (reactive orange 16, RO16) from aquatic environment was evaluated.					
34818636	5	0	theme	glass	717:721	arg1	beads					723:727	agar and glass beads	708:727	beads	723:727	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	8	1	theme	Microwave	1105:1113	arg1	maps					1128:1131	Microwave permittivity maps	1105:1131	Microwave permittivity maps	1105:1131	Microwave permittivity maps were obtained from the microwave system and compared to ultrasound images.					
34818636	1	2	theme	combined	151:158	arg1	microwave					160:168	combined microwave	151:168	combined microwave	151:168	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	5	3	theme	beads	723:727	arg1	base					700:703	a base	698:703	a base of agar and glass beads at different proportions	698:752	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	7	4	theme	microwave	1074:1082	arg1	system					1097:1102	a microwave transmission system	1072:1102	a microwave transmission system	1072:1102	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	3	5	theme	ultrasound	485:494	arg1	properties					510:519	the ultrasound and microwave properties	481:519	the ultrasound and microwave properties	481:519	First, 36 recipes were fabricated, and properties were measured to determine the relationship and possible interaction between ingredients with the ultrasound and microwave properties.					
34818636	2	6	theme	fat	324:326	arg1	tissues					328:334	skin and fat tissues	315:334	skin and fat tissues	315:334	Formulations based on coconut oil, canola oil, agar and glass beads were used to mimic skin and fat tissues.					
34818636	8	7	theme	permittivity	1115:1126	arg1	maps					1128:1131	Microwave permittivity maps	1105:1131	Microwave permittivity maps	1105:1131	Microwave permittivity maps were obtained from the microwave system and compared to ultrasound images.					
34818636	4	8	theme	tumour	659:664	arg1	tissues					666:672	tumour tissues	659:672	tumour tissues	659:672	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	7	9	with	phantoms	973:980	arg1	structures					1006:1015	different internal structures	987:1015	different internal structures	987:1015	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	3	10	theme	possible	435:442	arg1	interaction					444:454	possible interaction	435:454	possible interaction	435:454	First, 36 recipes were fabricated, and properties were measured to determine the relationship and possible interaction between ingredients with the ultrasound and microwave properties.					
34818636	9	11	theme	microwave	1286:1294	arg1	imaging					1311:1317	this microwave and ultrasound imaging	1281:1317	imaging	1311:1317	The structure and composition of the phantoms were all confirmed through this microwave and ultrasound imaging.					
34818636	7	12	theme	B-mode	1050:1055	arg1	ultrasound					1057:1066	B-mode ultrasound	1050:1066	B-mode ultrasound	1050:1066	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	1	13	theme	ultrasound	174:183	arg1	imaging					185:191	ultrasound imaging	174:191	ultrasound imaging	174:191	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	5	14	contain	contained	688:696	arg2	base					700:703	a base	698:703	a base of agar and glass beads at different proportions	698:752	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	5	14	contain	contained	688:696	arg1	phantoms					679:686	All phantoms	675:686	All phantoms	675:686	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	3	15	with	ingredients	464:474	arg1	properties					510:519	the ultrasound and microwave properties	481:519	the ultrasound and microwave properties	481:519	First, 36 recipes were fabricated, and properties were measured to determine the relationship and possible interaction between ingredients with the ultrasound and microwave properties.					
34818636	0	16	theme	Breast	0:5	arg1	tissue					7:12	Breast tissue	0:12	Breast tissue	0:12	Breast tissue mimicking phantoms for combined ultrasound and microwave imaging.					
34818636	9	17	theme	ultrasound	1300:1309	arg1	imaging					1311:1317	this microwave and ultrasound imaging	1281:1317	imaging	1311:1317	The structure and composition of the phantoms were all confirmed through this microwave and ultrasound imaging.					
34818636	9	18	theme	phantoms	1245:1252	arg1	composition					1226:1236	composition	1226:1236	composition	1226:1236	The structure and composition of the phantoms were all confirmed through this microwave and ultrasound imaging.					
34818636	9	18	theme	phantoms	1245:1252	arg1	structure					1212:1220	structure	1212:1220	structure	1212:1220	The structure and composition of the phantoms were all confirmed through this microwave and ultrasound imaging.					
34818636	6	19	theme	canola	916:921	arg1	oil					923:925	canola oil	916:925	canola oil for fat	916:933	Tumour and fibroglandular tissues were best mimicked by adding polyvinylpyrrolidone (PVP), while using coconut oil for skin and canola oil for fat produced the best results.					
34818636	1	20	theme	new	93:95	arg1	formulation					97:107	a new formulation	91:107	a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging	91:191	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	8	21	theme	microwave	1156:1164	arg1	system					1166:1171	the microwave system	1152:1171	the microwave system	1152:1171	Microwave permittivity maps were obtained from the microwave system and compared to ultrasound images.					
34818636	2	22	theme	glass	284:288	arg1	beads					290:294	glass beads	284:294	glass beads	284:294	Formulations based on coconut oil, canola oil, agar and glass beads were used to mimic skin and fat tissues.					
34818636	1	23	theme	breast	203:208	arg1	detection					217:225	breast cancer detection	203:225	breast cancer detection	203:225	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	0	24	theme	combined	37:44	arg1	ultrasound					46:55	combined ultrasound	37:55	combined ultrasound	37:55	Breast tissue mimicking phantoms for combined ultrasound and microwave imaging.					
34818636	7	25	theme	internal	997:1004	arg1	structures					1006:1015	different internal structures	987:1015	different internal structures	987:1015	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	1	26	theme	cancer	210:215	arg1	detection					217:225	breast cancer detection	203:225	breast cancer detection	203:225	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	5	27	theme	different	732:740	arg1	proportions					742:752	different proportions	732:752	different proportions	732:752	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	7	28	theme	transmission	1084:1095	arg1	system					1097:1102	a microwave transmission system	1072:1102	a microwave transmission system	1072:1102	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	6	29	theme	coconut	891:897	arg1	oil					899:901	coconut oil	891:901	coconut oil for skin	891:910	Tumour and fibroglandular tissues were best mimicked by adding polyvinylpyrrolidone (PVP), while using coconut oil for skin and canola oil for fat produced the best results.					
34818636	7	30	theme	different	987:995	arg1	structures					1006:1015	different internal structures	987:1015	different internal structures	987:1015	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	6	31	theme	fibroglandular	799:812	arg1	tissues					814:820	Tumour and fibroglandular tissues	788:820	tissues	814:820	Tumour and fibroglandular tissues were best mimicked by adding polyvinylpyrrolidone (PVP), while using coconut oil for skin and canola oil for fat produced the best results.					
34818636	4	32	located	found	601:605	arg2	fibroglandular					639:652	fibroglandular	639:652	fibroglandular	639:652	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	32	located	found	601:605	arg2	tissues					593:599	different tissues	583:599	different tissues	583:599	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	32	located	found	601:605	arg1	breast					610:615	breast	610:615	breast	610:615	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	32	located	found	601:605	arg2	fat					634:636	fat	634:636	fat	634:636	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	32	located	found	601:605	arg2	tissues					666:672	tumour tissues	659:672	tumour tissues	659:672	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	32	located	found	601:605	arg2	skin					628:631	skin	628:631	skin	628:631	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	2	33	theme	skin	315:318	arg1	tissues					328:334	skin and fat tissues	315:334	skin and fat tissues	315:334	Formulations based on coconut oil, canola oil, agar and glass beads were used to mimic skin and fat tissues.					
34818636	7	34	theme	final	967:971	arg1	phantoms					973:980	Five final phantoms	962:980	Five final phantoms with different internal structures	962:1015	Five final phantoms with different internal structures were fabricated and imaged using B-mode ultrasound and a microwave transmission system.					
34818636	2	35	theme	canola	263:268	arg1	oil					270:272	canola oil	263:272	canola oil	263:272	Formulations based on coconut oil, canola oil, agar and glass beads were used to mimic skin and fat tissues.					
34818636	1	36	theme	breast	115:120	arg1	phantom					139:145	a breast tissue-mimicking phantom	113:145	a breast tissue-mimicking phantom	113:145	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	4	37	theme	different	583:591	arg1	fat					634:636	fat	634:636	fat	634:636	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	37	theme	different	583:591	arg1	fibroglandular					639:652	fibroglandular	639:652	fibroglandular	639:652	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	37	theme	different	583:591	arg1	tissues					593:599	different tissues	583:599	different tissues	583:599	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	37	theme	different	583:591	arg1	tissues					666:672	tumour tissues	659:672	tumour tissues	659:672	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	4	37	theme	different	583:591	arg1	skin					628:631	skin	628:631	skin	628:631	Based on these results, the formulae were developed to mimic different tissues found in breast, including skin, fat, fibroglandular, and tumour tissues.					
34818636	6	38	theme	Tumour	788:793	arg1	tissues					814:820	Tumour and fibroglandular tissues	788:820	tissues	814:820	Tumour and fibroglandular tissues were best mimicked by adding polyvinylpyrrolidone (PVP), while using coconut oil for skin and canola oil for fat produced the best results.					
34818636	3	39	dep	relationship	418:429	arg1	the					414:416	the	414:416	the	414:416	First, 36 recipes were fabricated, and properties were measured to determine the relationship and possible interaction between ingredients with the ultrasound and microwave properties.					
34818636	1	40	theme	tissue-mimicking	122:137	arg1	phantom					139:145	a breast tissue-mimicking phantom	113:145	a breast tissue-mimicking phantom	113:145	We present a new formulation for a breast tissue-mimicking phantom for combined microwave and ultrasound imaging to assist breast cancer detection.					
34818636	8	41	theme	ultrasound	1189:1198	arg1	images					1200:1205	ultrasound images	1189:1205	ultrasound images	1189:1205	Microwave permittivity maps were obtained from the microwave system and compared to ultrasound images.					
34818636	9	42	dep	structure	1212:1220	arg1	The					1208:1210	The	1208:1210	The	1208:1210	The structure and composition of the phantoms were all confirmed through this microwave and ultrasound imaging.					
34818636	5	43	theme	agar	708:711	arg1	beads					723:727	agar and glass beads	708:727	beads	723:727	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	2	44	used	used	301:304	arg2	Formulations					228:239	Formulations	228:239	Formulations based on coconut oil, canola oil, agar and glass beads	228:294	Formulations based on coconut oil, canola oil, agar and glass beads were used to mimic skin and fat tissues.					
34818636	0	45	theme	microwave	61:69	arg1	imaging					71:77	microwave imaging	61:77	microwave imaging	61:77	Breast tissue mimicking phantoms for combined ultrasound and microwave imaging.					
34818636	5	46	from	proportions	742:752	arg1	base					700:703	a base	698:703	a base of agar and glass beads at different proportions	698:752	All phantoms contained a base of agar and glass beads at different proportions depending on the tissue mimicked.					
34818636	2	47	theme	coconut	250:256	arg1	oil					258:260	coconut oil	250:260	coconut oil	250:260	Formulations based on coconut oil, canola oil, agar and glass beads were used to mimic skin and fat tissues.					
34818636	3	48	theme	microwave	500:508	arg1	properties					510:519	the ultrasound and microwave properties	481:519	the ultrasound and microwave properties	481:519	First, 36 recipes were fabricated, and properties were measured to determine the relationship and possible interaction between ingredients with the ultrasound and microwave properties.					
34818636	6	49	theme	best	948:951	arg1	results					953:959	the best results	944:959	the best results	944:959	Tumour and fibroglandular tissues were best mimicked by adding polyvinylpyrrolidone (PVP), while using coconut oil for skin and canola oil for fat produced the best results.					
34390125	5	0	from	polymorphisms	696:708	arg1	FTO					796:798	FTO (rs9936385)	796:810	FTO (rs9936385)	796:810	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385) were evaluated by genotyping.					
34390125	5	0	from	polymorphisms	696:708	arg1	ADAMTSL3					771:778	ADAMTSL3 (rs4842924)	771:790	ADAMTSL3 (rs4842924)	771:790	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385) were evaluated by genotyping.					
34390125	5	0	from	polymorphisms	696:708	arg1	VCAN					753:756	VCAN (rs2287926)	753:768	VCAN (rs2287926)	753:768	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385) were evaluated by genotyping.					
34390125	5	0	from	polymorphisms	696:708	arg1	HSD17B11					731:738	HSD17B11 (rs9991501)	731:750	HSD17B11 (rs9991501)	731:750	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385) were evaluated by genotyping.					
34390125	5	0	from	polymorphisms	696:708	arg1	IRS1					713:716	IRS1 (rs2943656)	713:728	IRS1 (rs2943656)	713:728	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385) were evaluated by genotyping.					
34390125	8	1	theme	muscle	1119:1124	arg1	mass					1126:1129	skeletal muscle mass	1110:1129	skeletal muscle mass	1110:1129	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	10	2	theme	diabetes	1649:1656	arg1	patients					1658:1665	diabetes patients	1649:1665	diabetes patients	1649:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	9	3	theme	factors	1360:1366	arg1	contribution					1336:1347	the contribution	1332:1347	the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients	1332:1499	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	1	4	from	composition	270:280	arg1	diabetes					292:299	type 2 diabetes	285:299	type 2 diabetes	285:299	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	8	5	dep	identified	1068:1077	arg1	β = 0.11					1132:1139	β = 0.11	1132:1139	β = 0.11	1132:1139	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	1	6	theme	healthy	166:172	arg1	individuals					174:184	healthy individuals	166:184	healthy individuals	166:184	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	10	7	from	patients	1658:1665	arg1	prediction					1579:1588	prediction	1579:1588	prediction	1579:1588	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	7	from	patients	1658:1665	arg1	prevention					1591:1600	prevention	1591:1600	prevention	1591:1600	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	7	from	patients	1658:1665	arg1	intervention					1607:1618	intervention	1607:1618	intervention	1607:1618	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	8	8	theme	body	1209:1212	arg1	P = 0.0095					1234:1243	P = 0.0095	1234:1243	P = 0.0095	1234:1243	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	8	theme	body	1209:1212	arg1	mass					1218:1221	body fat mass	1209:1221	body fat mass (β = 0.15, P = 0.0095)	1209:1244	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	3	9	theme	type 2	509:514	arg1	diabetes					516:523	type 2 diabetes	509:523	type 2 diabetes	509:523	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	6	10	theme	body	898:901	arg1	composition					903:913	body composition	898:913	body composition	898:913	The contributions of single-nucleotide polymorphisms to body composition were examined, considering known clinical determinants.					
34390125	0	11	from	composition	64:74	arg1	diabetes					86:93	type 2 diabetes	79:93	type 2 diabetes	79:93	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	4	12	theme	bioimpedance	606:617	arg1	analysis					619:626	bioimpedance analysis	606:626	bioimpedance analysis with a commercially available device (InBody770)	606:675	Body composition was measured using bioimpedance analysis with a commercially available device (InBody770).					
34390125	10	13	theme	effective	1553:1561	arg1	methods					1563:1569	effective methods	1553:1569	effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients	1553:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	11	14	dep	Int	1685:1687	arg1	21					1695:1696	21	1695:1696	21	1695:1696	Geriatr Gerontol Int 2021; 21: 932-938.					
34390125	11	14	dep	Int	1685:1687	arg1	932-938					1699:1705	932-938	1699:1705	932-938	1699:1705	Geriatr Gerontol Int 2021; 21: 932-938.					
34390125	3	15	theme	cross-sectional	546:560	arg1	design					562:567	a cross-sectional design	544:567	a cross-sectional design	544:567	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	2	16	with	individuals	403:413	arg1	diabetes					427:434	type 2 diabetes	420:434	type 2 diabetes	420:434	Here, we aimed to clarify the genetic determinants of body composition in individuals with type 2 diabetes.					
34390125	1	17	theme	association	201:211	arg1	studies					213:219	genome-wide association studies	189:219	genome-wide association studies	189:219	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	6	18	theme	polymorphisms	881:893	arg1	contributions					846:858	The contributions	842:858	The contributions of single-nucleotide polymorphisms to body composition	842:913	The contributions of single-nucleotide polymorphisms to body composition were examined, considering known clinical determinants.					
34390125	9	19	theme	body	1422:1425	arg1	composition					1427:1437	body composition	1422:1437	body composition	1422:1437	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	9	20	dep	factors	1360:1366	arg1	ADAMTSL3					1379:1386	ADAMTSL3	1379:1386	ADAMTSL3	1379:1386	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	9	20	dep	factors	1360:1366	arg1	IRS1					1370:1373	IRS1	1370:1373	IRS1	1370:1373	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	9	20	dep	factors	1360:1366	arg1	factors					1360:1366	genetic factors	1352:1366	genetic factors - IRS1 and ADAMTSL3 -	1352:1388	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	7	21	theme	body	984:987	arg1	composition					989:999	body composition	984:999	body composition	984:999	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	7	21	theme	body	984:987	arg1	RESULTS					971:977	RESULTS Sex, body composition and age	971:1007	RESULTS Sex, body composition and age	971:1007	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	1	22	theme	related	121:127	arg1	loci					116:119	AIM Several genetic loci	96:119	AIM Several genetic loci related to lean mass	96:140	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	11	23	theme	Geriatr	1668:1674	arg1	Int					1685:1687	Geriatr Gerontol Int 2021	1668:1692	Geriatr Gerontol Int 2021; 21: 932-938.	1668:1706	Geriatr Gerontol Int 2021; 21: 932-938.					
34390125	1	24	theme	lean	132:135	arg1	mass					137:140	lean mass	132:140	lean mass	132:140	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	8	25	theme	lean	1263:1266	arg1	mass					1268:1271	appendicular lean mass	1250:1271	appendicular lean mass (β = -0.13, P = 0.017)	1250:1294	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	9	26	theme	factors	1464:1470	arg1	independent					1440:1450	independent	1440:1450	independent	1440:1450	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	6	27	theme	known	942:946	arg1	determinants					957:968	known clinical determinants	942:968	known clinical determinants	942:968	The contributions of single-nucleotide polymorphisms to body composition were examined, considering known clinical determinants.					
34390125	0	28	theme	type 2	79:84	arg1	diabetes					86:93	type 2 diabetes	79:93	type 2 diabetes	79:93	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	9	29	dep	CONCLUSIONS	1297:1307	arg1	clarified					1322:1330	clarified	1322:1330	clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients	1322:1499	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	2	30	theme	genetic	359:365	arg1	determinants					367:378	the genetic determinants	355:378	the genetic determinants of body composition in individuals with type 2 diabetes	355:434	Here, we aimed to clarify the genetic determinants of body composition in individuals with type 2 diabetes.					
34390125	9	31	theme	diabetes	1483:1490	arg1	patients					1492:1499	type 2 diabetes patients	1476:1499	type 2 diabetes patients	1476:1499	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	2	32	theme	type 2	420:425	arg1	diabetes					427:434	type 2 diabetes	420:434	type 2 diabetes	420:434	Here, we aimed to clarify the genetic determinants of body composition in individuals with type 2 diabetes.					
34390125	1	33	theme	loci	257:260	arg1	contribution					235:246	the contribution	231:246	the contribution of these loci to body composition in type 2 diabetes	231:299	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	2	34	theme	composition	388:398	arg1	determinants					367:378	the genetic determinants	355:378	the genetic determinants of body composition in individuals with type 2 diabetes	355:434	Here, we aimed to clarify the genetic determinants of body composition in individuals with type 2 diabetes.					
34390125	0	35	theme	genetic	21:27	arg1	loci					29:32	genetic loci	21:32	genetic loci related to lean mass and body composition in type 2 diabetes	21:93	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	1	36	theme	body	265:268	arg1	composition					270:280	body composition	265:280	body composition in type 2 diabetes	265:299	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	1	37	theme	AIM	96:98	arg1	loci					116:119	AIM Several genetic loci	96:119	AIM Several genetic loci related to lean mass	96:140	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	0	38	theme	related	34:40	arg1	loci					29:32	genetic loci	21:32	genetic loci related to lean mass and body composition in type 2 diabetes	21:93	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	4	39	with	analysis	619:626	arg1	InBody770					666:674	InBody770	666:674	InBody770	666:674	Body composition was measured using bioimpedance analysis with a commercially available device (InBody770).					
34390125	4	39	with	analysis	619:626	arg1	device					658:663	a commercially available device	633:663	a commercially available device (InBody770)	633:675	Body composition was measured using bioimpedance analysis with a commercially available device (InBody770).					
34390125	10	40	theme	frailty	1638:1644	arg1	prediction					1579:1588	prediction	1579:1588	prediction	1579:1588	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	40	theme	frailty	1638:1644	arg1	prevention					1591:1600	prevention	1591:1600	prevention	1591:1600	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	40	theme	frailty	1638:1644	arg1	intervention					1607:1618	intervention	1607:1618	intervention	1607:1618	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	0	41	theme	lean	45:48	arg1	mass					50:53	lean mass	45:53	lean mass	45:53	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	10	42	theme	sarcopenia	1623:1632	arg1	prediction					1579:1588	prediction	1579:1588	prediction	1579:1588	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	42	theme	sarcopenia	1623:1632	arg1	prevention					1591:1600	prevention	1591:1600	prevention	1591:1600	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	42	theme	sarcopenia	1623:1632	arg1	intervention					1607:1618	intervention	1607:1618	intervention	1607:1618	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	43	from	intervention	1607:1618	arg1	patients					1658:1665	diabetes patients	1649:1665	diabetes patients	1649:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	4	44	theme	Body	570:573	arg1	composition					575:585	Body composition	570:585	Body composition	570:585	Body composition was measured using bioimpedance analysis with a commercially available device (InBody770).					
34390125	3	45	with	outpatients	469:479	arg1	diabetes					516:523	type 2 diabetes	509:523	type 2 diabetes	509:523	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	3	46	theme	outpatients	469:479	arg1	total					447:451	A total	445:451	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes	437:523	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	10	47	from	sarcopenia	1623:1632	arg1	patients					1658:1665	diabetes patients	1649:1665	diabetes patients	1649:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	9	48	theme	genetic	1352:1358	arg1	ADAMTSL3					1379:1386	ADAMTSL3	1379:1386	ADAMTSL3	1379:1386	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	9	48	theme	genetic	1352:1358	arg1	IRS1					1370:1373	IRS1	1370:1373	IRS1	1370:1373	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	9	48	theme	genetic	1352:1358	arg1	factors					1360:1366	genetic factors	1352:1366	genetic factors - IRS1 and ADAMTSL3 -	1352:1388	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	8	49	theme	skeletal	1110:1117	arg1	mass					1126:1129	skeletal muscle mass	1110:1129	skeletal muscle mass	1110:1129	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	10	50	from	frailty	1638:1644	arg1	patients					1658:1665	diabetes patients	1649:1665	diabetes patients	1649:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	8	51	theme	mass	1126:1129	arg1	predictor					1097:1105	an independent predictor	1082:1105	an independent predictor of skeletal muscle mass	1082:1129	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	51	theme	mass	1126:1129	arg1	IRS1					1049:1052	IRS1 rs2934656	1049:1062	IRS1 rs2934656	1049:1062	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	9	52	from	composition	1427:1437	arg1	patients					1492:1499	type 2 diabetes patients	1476:1499	type 2 diabetes patients	1476:1499	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	7	53	theme	clinical	1028:1035	arg1	RESULTS					971:977	RESULTS Sex, body composition and age	971:1007	RESULTS Sex, body composition and age	971:1007	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	7	53	theme	clinical	1028:1035	arg1	predictors					1037:1046	clinical predictors	1028:1046	clinical predictors	1028:1046	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	8	54	theme	independent	1184:1194	arg1	predictor					1196:1204	an independent predictor	1181:1204	an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017)	1181:1294	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	54	theme	independent	1184:1194	arg1	rs4842924					1167:1175	ADAMTSL3 rs4842924	1158:1175	ADAMTSL3 rs4842924	1158:1175	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	10	55	theme	methods	1563:1569	arg1	establishment					1536:1548	the establishment	1532:1548	the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients	1532:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	5	56	theme	Single-nucleotide	678:694	arg1	polymorphisms					696:708	Single-nucleotide polymorphisms	678:708	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385)	678:810	Single-nucleotide polymorphisms in IRS1 (rs2943656), HSD17B11 (rs9991501), VCAN (rs2287926), ADAMTSL3 (rs4842924) and FTO (rs9936385) were evaluated by genotyping.					
34390125	1	57	theme	genome-wide	189:199	arg1	studies					213:219	genome-wide association studies	189:219	genome-wide association studies	189:219	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	8	58	theme	fat	1214:1216	arg1	P = 0.0095					1234:1243	P = 0.0095	1234:1243	P = 0.0095	1234:1243	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	58	theme	fat	1214:1216	arg1	mass					1218:1221	body fat mass	1209:1221	body fat mass (β = 0.15, P = 0.0095)	1209:1244	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	1	59	theme	Several	100:106	arg1	loci					116:119	AIM Several genetic loci	96:119	AIM Several genetic loci related to lean mass	96:140	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	8	60	theme	ADAMTSL3	1158:1165	arg1	predictor					1196:1204	an independent predictor	1181:1204	an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017)	1181:1294	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	60	theme	ADAMTSL3	1158:1165	arg1	rs4842924					1167:1175	ADAMTSL3 rs4842924	1158:1175	ADAMTSL3 rs4842924	1158:1175	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	6	61	theme	single-nucleotide	863:879	arg1	polymorphisms					881:893	single-nucleotide polymorphisms	863:893	single-nucleotide polymorphisms	863:893	The contributions of single-nucleotide polymorphisms to body composition were examined, considering known clinical determinants.					
34390125	0	62	theme	body	59:62	arg1	composition					64:74	body composition	59:74	body composition	59:74	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	9	63	theme	independent	1440:1450	arg1	composition					1427:1437	body composition	1422:1437	body composition	1422:1437	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	1	64	theme	genetic	108:114	arg1	loci					116:119	AIM Several genetic loci	96:119	AIM Several genetic loci related to lean mass	96:140	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	4	65	theme	available	648:656	arg1	InBody770					666:674	InBody770	666:674	InBody770	666:674	Body composition was measured using bioimpedance analysis with a commercially available device (InBody770).					
34390125	4	65	theme	available	648:656	arg1	device					658:663	a commercially available device	633:663	a commercially available device (InBody770)	633:675	Body composition was measured using bioimpedance analysis with a commercially available device (InBody770).					
34390125	11	66	theme	Gerontol	1676:1683	arg1	Int					1685:1687	Geriatr Gerontol Int 2021	1668:1692	Geriatr Gerontol Int 2021; 21: 932-938.	1668:1706	Geriatr Gerontol Int 2021; 21: 932-938.					
34390125	9	67	theme	clinical	1455:1462	arg1	factors					1464:1470	clinical factors	1455:1470	clinical factors	1455:1470	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	6	68	theme	clinical	948:955	arg1	determinants					957:968	known clinical determinants	942:968	known clinical determinants	942:968	The contributions of single-nucleotide polymorphisms to body composition were examined, considering known clinical determinants.					
34390125	8	69	theme	appendicular	1250:1261	arg1	mass					1268:1271	appendicular lean mass	1250:1271	appendicular lean mass (β = -0.13, P = 0.017)	1250:1294	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	0	70	from	mass	50:53	arg1	diabetes					86:93	type 2 diabetes	79:93	type 2 diabetes	79:93	Associations between genetic loci related to lean mass and body composition in type 2 diabetes.					
34390125	8	71	theme	mass	1268:1271	arg1	predictor					1196:1204	an independent predictor	1181:1204	an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017)	1181:1294	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	71	theme	mass	1268:1271	arg1	rs4842924					1167:1175	ADAMTSL3 rs4842924	1158:1175	ADAMTSL3 rs4842924	1158:1175	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	9	72	theme	type 2	1476:1481	arg1	patients					1492:1499	type 2 diabetes patients	1476:1499	type 2 diabetes patients	1476:1499	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	7	73	dep	RESULTS	971:977	arg1	composition					989:999	body composition	984:999	body composition	984:999	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	7	73	dep	RESULTS	971:977	arg1	RESULTS					971:977	RESULTS Sex, body composition and age	971:1007	RESULTS Sex, body composition and age	971:1007	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	7	73	dep	RESULTS	971:977	arg1	age					1005:1007	age	1005:1007	age	1005:1007	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	7	73	dep	RESULTS	971:977	arg1	Sex					979:981	Sex	979:981	Sex	979:981	RESULTS Sex, body composition and age were identified as clinical predictors.					
34390125	3	74	dep	outpatients	469:479	arg1	men					499:501	106 men	495:501	106 men	495:501	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	3	74	dep	outpatients	469:479	arg1	women					485:489	70 women	482:489	70 women	482:489	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	2	75	from	determinants	367:378	arg1	individuals					403:413	individuals	403:413	individuals with type 2 diabetes	403:434	Here, we aimed to clarify the genetic determinants of body composition in individuals with type 2 diabetes.					
34390125	2	76	theme	body	383:386	arg1	composition					388:398	body composition	383:398	body composition	383:398	Here, we aimed to clarify the genetic determinants of body composition in individuals with type 2 diabetes.					
34390125	8	77	theme	mass	1218:1221	arg1	predictor					1196:1204	an independent predictor	1181:1204	an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017)	1181:1294	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	77	theme	mass	1218:1221	arg1	rs4842924					1167:1175	ADAMTSL3 rs4842924	1158:1175	ADAMTSL3 rs4842924	1158:1175	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	1	78	theme	type 2	285:290	arg1	diabetes					292:299	type 2 diabetes	285:299	type 2 diabetes	285:299	AIM Several genetic loci related to lean mass have been identified in healthy individuals by genome-wide association studies; however, the contribution of these loci to body composition in type 2 diabetes remains to be investigated.					
34390125	9	79	theme	interindividual	1393:1407	arg1	variation					1409:1417	interindividual variation	1393:1417	interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients	1393:1499	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	9	80	from	variation	1409:1417	arg1	composition					1427:1437	body composition	1422:1437	body composition	1422:1437	CONCLUSIONS The findings clarified the contribution of genetic factors - IRS1 and ADAMTSL3 - to interindividual variation in body composition, independent of clinical factors, in type 2 diabetes patients.					
34390125	3	81	theme	Japanese	460:467	arg1	outpatients					469:479	176 Japanese outpatients	456:479	176 Japanese outpatients (70 women and 106 men) with type 2 diabetes	456:523	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	10	82	from	prediction	1579:1588	arg1	patients					1658:1665	diabetes patients	1649:1665	diabetes patients	1649:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	8	83	dep	β = 0.11	1132:1139	arg1	P = 0.026					1142:1150	P = 0.026	1142:1150	P = 0.026	1142:1150	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	84	theme	independent	1085:1095	arg1	predictor					1097:1105	an independent predictor	1082:1105	an independent predictor of skeletal muscle mass	1082:1129	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	84	theme	independent	1085:1095	arg1	IRS1					1049:1052	IRS1 rs2934656	1049:1062	IRS1 rs2934656	1049:1062	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	85	dep	mass	1268:1271	arg1	β = -0.13					1274:1282	β = -0.13	1274:1282	β = -0.13	1274:1282	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	8	85	dep	mass	1268:1271	arg1	P = 0.017					1285:1293	P = 0.017	1285:1293	P = 0.017	1285:1293	IRS1 rs2934656 was identified as an independent predictor of skeletal muscle mass (β = 0.11, P = 0.026), and ADAMTSL3 rs4842924 was an independent predictor of body fat mass (β = 0.15, P = 0.0095) and appendicular lean mass (β = -0.13, P = 0.017).					
34390125	3	86	dep	METHODS	437:443	arg1	total					447:451	A total	445:451	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes	437:523	METHODS A total of 176 Japanese outpatients (70 women and 106 men) with type 2 diabetes were studied using a cross-sectional design.					
34390125	10	87	from	prevention	1591:1600	arg1	patients					1658:1665	diabetes patients	1649:1665	diabetes patients	1649:1665	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34390125	10	88	dep	prediction	1579:1588	arg1	the					1575:1577	the	1575:1577	the	1575:1577	These data will contribute to the establishment of effective methods for the prediction, prevention, and intervention of sarcopenia and frailty in diabetes patients.					
34053305	6	0	theme	alginate	899:906	arg1	composite					908:916	the magnesium calcium phosphate/sodium alginate composite	860:916	the magnesium calcium phosphate/sodium alginate composite	860:916	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	2	1	theme	biological	360:369	arg1	properties					371:380	The physicochemical and biological properties	336:380	The physicochemical and biological properties of the cement in vitro	336:403	The physicochemical and biological properties of the cement in vitro were fully characterized.					
34053305	5	2	theme	Cell	635:638	arg1	experiments					640:650	Cell experiments	635:650	Cell experiments	635:650	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	1	3	theme	proper	263:268	arg1	5-24 min					284:291	5-24 min	284:291	5-24 min	284:291	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	1	3	theme	proper	263:268	arg1	time					278:281	a proper setting time	261:281	a proper setting time (5-24 min)	261:292	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	0	4	theme	high	85:88	arg1	strength					90:97	high strength	85:97	high strength	85:97	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	6	5	theme	phosphate/sodium	882:897	arg1	composite					908:916	the magnesium calcium phosphate/sodium alginate composite	860:916	the magnesium calcium phosphate/sodium alginate composite	860:916	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	1	6	theme	setting	270:276	arg1	5-24 min					284:291	5-24 min	284:291	5-24 min	284:291	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	1	6	theme	setting	270:276	arg1	time					278:281	a proper setting time	261:281	a proper setting time (5-24 min)	261:292	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	0	7	theme	proper	103:108	arg1	time					123:126	proper self-setting time	103:126	proper self-setting time	103:126	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	6	8	theme	calcium	874:880	arg1	composite					908:916	the magnesium calcium phosphate/sodium alginate composite	860:916	the magnesium calcium phosphate/sodium alginate composite	860:916	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	6	9	theme	magnesium	864:872	arg1	composite					908:916	the magnesium calcium phosphate/sodium alginate composite	860:916	the magnesium calcium phosphate/sodium alginate composite	860:916	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	6	10	theme	candidate	970:978	arg1	materials					980:988	potential bone repair candidate materials	948:988	potential bone repair candidate materials	948:988	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	3	11	theme	soaking	491:497	arg1	increase					504:511	the soaking time increase	487:511	the soaking time increase	487:511	The composite cements could gradually degrade in PBS as the soaking time increase, and the weight loss reached 20.74% by the end of 56th day.					
34053305	0	12	with	cement	73:78	arg1	strength					90:97	high strength	85:97	high strength	85:97	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	0	12	with	cement	73:78	arg1	time					123:126	proper self-setting time	103:126	proper self-setting time	103:126	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	3	13	theme	weight	522:527	arg1	loss					529:532	the weight loss	518:532	the weight loss	518:532	The composite cements could gradually degrade in PBS as the soaking time increase, and the weight loss reached 20.74% by the end of 56th day.					
34053305	2	14	theme	cement	389:394	arg1	properties					371:380	The physicochemical and biological properties	336:380	The physicochemical and biological properties of the cement in vitro	336:403	The physicochemical and biological properties of the cement in vitro were fully characterized.					
34053305	1	15	theme	novel	159:163	arg1	composite					209:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	0	16	theme	self-setting	110:121	arg1	time					123:126	proper self-setting time	103:126	proper self-setting time	103:126	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	1	17	theme	magnesium	165:173	arg1	composite					209:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	5	18	theme	bone	777:780	arg1	MSCs					813:816	MSCs	813:816	MSCs	813:816	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	18	theme	bone	777:780	arg1	cells					806:810	the mouse bone marrow mesenchymal stem cells	767:810	the mouse bone marrow mesenchymal stem cells (MSCs)	767:817	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	1	19	theme	calcium	175:181	arg1	composite					209:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	5	20	theme	marrow	782:787	arg1	MSCs					813:816	MSCs	813:816	MSCs	813:816	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	20	theme	marrow	782:787	arg1	cells					806:810	the mouse bone marrow mesenchymal stem cells	767:810	the mouse bone marrow mesenchymal stem cells (MSCs)	767:817	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	6	21	theme	repair	963:968	arg1	materials					980:988	potential bone repair candidate materials	948:988	potential bone repair candidate materials	948:988	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	1	22	theme	phosphate/sodium	183:198	arg1	composite					209:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	0	23	theme	magnesium	19:27	arg1	cement					73:78	a novel magnesium calcium phosphate/sodium alginate composite cement	11:78	a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair	11:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	6	24	theme	bone	958:961	arg1	materials					980:988	potential bone repair candidate materials	948:988	potential bone repair candidate materials	948:988	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	0	25	theme	bone	132:135	arg1	repair					137:142	bone repair	132:142	bone repair	132:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	2	26	from	properties	371:380	arg1	vitro					399:403	vitro	399:403	vitro	399:403	The physicochemical and biological properties of the cement in vitro were fully characterized.					
34053305	1	27	theme	alginate	200:207	arg1	composite					209:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	novel magnesium calcium phosphate/sodium alginate composite	159:217	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	0	28	theme	novel	13:17	arg1	cement					73:78	a novel magnesium calcium phosphate/sodium alginate composite cement	11:78	a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair	11:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	6	29	theme	potential	948:956	arg1	materials					980:988	potential bone repair candidate materials	948:988	potential bone repair candidate materials	948:988	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	4	30	from	deposition	602:611	arg1	SBF					630:632	SBF	630:632	SBF	630:632	The cements could induce the deposition of Ca-P layer in SBF.					
34053305	0	31	theme	phosphate/sodium	37:52	arg1	cement					73:78	a novel magnesium calcium phosphate/sodium alginate composite cement	11:78	a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair	11:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	1	32	theme	high	298:301	arg1	strength					315:322	high compressive strength	298:322	high compressive strength (91.1 MPa)	298:333	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	1	32	theme	high	298:301	arg1	91.1 MPa					325:332	91.1 MPa	325:332	91.1 MPa	325:332	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	6	33	theme	preliminary	826:836	arg1	results					838:844	These preliminary results	820:844	These preliminary results	820:844	These preliminary results indicate that the magnesium calcium phosphate/sodium alginate composite cements could be promising as potential bone repair candidate materials.					
34053305	3	34	theme	day	568:570	arg1	end					556:558	the end	552:558	the end of 56th day	552:570	The composite cements could gradually degrade in PBS as the soaking time increase, and the weight loss reached 20.74% by the end of 56th day.					
34053305	4	35	theme	Ca-P	616:619	arg1	layer					621:625	Ca-P layer	616:625	Ca-P layer	616:625	The cements could induce the deposition of Ca-P layer in SBF.					
34053305	0	36	theme	calcium	29:35	arg1	cement					73:78	a novel magnesium calcium phosphate/sodium alginate composite cement	11:78	a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair	11:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	1	37	theme	compressive	303:313	arg1	strength					315:322	high compressive strength	298:322	high compressive strength (91.1 MPa)	298:333	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	1	37	theme	compressive	303:313	arg1	91.1 MPa					325:332	91.1 MPa	325:332	91.1 MPa	325:332	In this work, novel magnesium calcium phosphate/sodium alginate composite cements were successfully fabricated with a proper setting time (5-24 min) and high compressive strength (91.1 MPa).					
34053305	3	38	dep	composite	435:443	arg1	cements					445:451	cements	445:451	cements	445:451	The composite cements could gradually degrade in PBS as the soaking time increase, and the weight loss reached 20.74% by the end of 56th day.					
34053305	2	39	from	cement	389:394	arg1	vitro					399:403	vitro	399:403	vitro	399:403	The physicochemical and biological properties of the cement in vitro were fully characterized.					
34053305	5	40	theme	mesenchymal	789:799	arg1	MSCs					813:816	MSCs	813:816	MSCs	813:816	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	40	theme	mesenchymal	789:799	arg1	cells					806:810	the mouse bone marrow mesenchymal stem cells	767:810	the mouse bone marrow mesenchymal stem cells (MSCs)	767:817	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	0	41	theme	composite	63:71	arg1	cement					73:78	a novel magnesium calcium phosphate/sodium alginate composite cement	11:78	a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair	11:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	5	42	theme	composite	684:692	arg1	extracts					668:675	the extracts	664:675	the extracts of the composite cements	664:700	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	43	theme	mouse	771:775	arg1	MSCs					813:816	MSCs	813:816	MSCs	813:816	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	43	theme	mouse	771:775	arg1	cells					806:810	the mouse bone marrow mesenchymal stem cells	767:810	the mouse bone marrow mesenchymal stem cells (MSCs)	767:817	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	2	44	theme	physicochemical	340:354	arg1	properties					371:380	The physicochemical and biological properties	336:380	The physicochemical and biological properties of the cement in vitro	336:403	The physicochemical and biological properties of the cement in vitro were fully characterized.					
34053305	0	45	theme	alginate	54:61	arg1	cement					73:78	a novel magnesium calcium phosphate/sodium alginate composite cement	11:78	a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair	11:142	Developing a novel magnesium calcium phosphate/sodium alginate composite cement with high strength and proper self-setting time for bone repair.					
34053305	5	46	dep	proliferation	730:742	arg1	the					726:728	the	726:728	the	726:728	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	2	47	from	vitro	399:403	arg1	properties					371:380	The physicochemical and biological properties	336:380	The physicochemical and biological properties of the cement in vitro	336:403	The physicochemical and biological properties of the cement in vitro were fully characterized.					
34053305	3	48	theme	time	499:502	arg1	increase					504:511	the soaking time increase	487:511	the soaking time increase	487:511	The composite cements could gradually degrade in PBS as the soaking time increase, and the weight loss reached 20.74% by the end of 56th day.					
34053305	5	49	dep	composite	684:692	arg1	cements					694:700	cements	694:700	cements	694:700	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	4	50	dep	The	573:575	arg1	cements					577:583	cements	577:583	cements	577:583	The cements could induce the deposition of Ca-P layer in SBF.					
34053305	5	51	theme	cells	806:810	arg1	differentiation					748:762	differentiation	748:762	differentiation	748:762	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	51	theme	cells	806:810	arg1	proliferation					730:742	proliferation	730:742	proliferation	730:742	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	4	52	theme	layer	621:625	arg1	deposition					602:611	the deposition	598:611	the deposition of Ca-P layer in SBF	598:632	The cements could induce the deposition of Ca-P layer in SBF.					
34053305	5	53	theme	stem	801:804	arg1	MSCs					813:816	MSCs	813:816	MSCs	813:816	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34053305	5	53	theme	stem	801:804	arg1	cells					806:810	the mouse bone marrow mesenchymal stem cells	767:810	the mouse bone marrow mesenchymal stem cells (MSCs)	767:817	Cell experiments proved that the extracts of the composite cements can effectively promote the proliferation and differentiation of the mouse bone marrow mesenchymal stem cells (MSCs).					
34486007	0	0	theme	male	79:82	arg1	mice					84:87	male mice	79:87	male mice	79:87	Effects of long-term consumption of sucralose associated with high-fat diet in male mice.					
34486007	4	1	theme	voracity	606:613	arg1	test					615:618	a voracity test	604:618	a voracity test	604:618	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	4	2	theme	body	497:500	arg1	composition					502:512	final body composition	491:512	final body composition	491:512	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	7	3	from	outcomes	1064:1071	arg1	relation					1076:1083	relation	1076:1083	relation to weight gain	1076:1098	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	6	4	from	improvements	907:918	arg1	steatosis					953:961	hepatic steatosis	945:961	hepatic steatosis	945:961	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	6	4	from	improvements	907:918	arg1	homeostasis					932:942	glycemic homeostasis	923:942	glycemic homeostasis	923:942	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	6	4	from	improvements	907:918	arg1	expenditure					985:995	increased energy expenditure	968:995	increased energy expenditure	968:995	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	7	5	theme	controversial	1158:1170	arg1	data					1172:1175	the controversial data	1154:1175	the controversial data in previous studies	1154:1195	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	2	6	theme	glucose	296:302	arg1	homeostasis					304:314	glucose homeostasis	296:314	glucose homeostasis	296:314	Studies have shown that some NNS can favor weight gain by altering the intestinal microbiota, satiety hormone production, or aspects related to glucose homeostasis.					
34486007	7	7	theme	different	1119:1127	arg1	diets					1129:1133	different diets	1119:1133	different diets	1119:1133	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	7	8	theme	previous	1180:1187	arg1	studies					1189:1195	previous studies	1180:1195	previous studies	1180:1195	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	7	9	theme	sucralose	1023:1031	arg1	consumption					1033:1043	sucralose consumption	1023:1043	sucralose consumption	1023:1043	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	7	10	from	data	1172:1175	arg1	studies					1189:1195	previous studies	1180:1195	previous studies	1180:1195	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	2	11	theme	hormone	254:260	arg1	production					262:271	satiety hormone production	246:271	satiety hormone production	246:271	Studies have shown that some NNS can favor weight gain by altering the intestinal microbiota, satiety hormone production, or aspects related to glucose homeostasis.					
34486007	6	12	theme	sucralose	869:877	arg1	supplementation					879:893	sucralose supplementation	869:893	sucralose supplementation	869:893	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	5	13	from	gain	795:798	arg1	mice					803:806	mice	803:806	mice fed with normal diet	803:827	We observed that sucralose supplementation reduced weight gain in HFD-fed mice but did not change weight gain in mice fed with normal diet.					
34486007	2	14	theme	satiety	246:252	arg1	production					262:271	satiety hormone production	246:271	satiety hormone production	246:271	Studies have shown that some NNS can favor weight gain by altering the intestinal microbiota, satiety hormone production, or aspects related to glucose homeostasis.					
34486007	5	15	theme	normal	817:822	arg1	diet					824:827	normal diet	817:827	normal diet	817:827	We observed that sucralose supplementation reduced weight gain in HFD-fed mice but did not change weight gain in mice fed with normal diet.					
34486007	0	16	theme	sucralose	36:44	arg1	consumption					21:31	long-term consumption	11:31	long-term consumption of sucralose	11:44	Effects of long-term consumption of sucralose associated with high-fat diet in male mice.					
34486007	6	17	theme	glycemic	923:930	arg1	homeostasis					932:942	glycemic homeostasis	923:942	glycemic homeostasis	923:942	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	6	18	theme	energy	978:983	arg1	expenditure					985:995	increased energy expenditure	968:995	increased energy expenditure	968:995	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	5	19	theme	sucralose	707:715	arg1	supplementation					717:731	sucralose supplementation	707:731	sucralose supplementation	707:731	We observed that sucralose supplementation reduced weight gain in HFD-fed mice but did not change weight gain in mice fed with normal diet.					
34486007	3	20	from	effects	352:358	arg1	mice					399:402	mice	399:402	mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks)	399:475	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	4	21	theme	skeletal	642:649	arg1	muscles					651:657	skeletal muscles	642:657	skeletal muscles	642:657	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	6	22	theme	increased	968:976	arg1	expenditure					985:995	increased energy expenditure	968:995	increased energy expenditure	968:995	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	3	23	theme	consumption	384:394	arg1	effects					352:358	the effects	348:358	the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks)	348:475	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	4	24	theme	Weight	478:483	arg1	gain					485:488	Weight gain	478:488	Weight gain	478:488	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	7	25	theme	clinical	1230:1237	arg1	research					1239:1246	future clinical research	1223:1246	future clinical research aimed at clarifying the impact of NNS consumption on human health	1223:1312	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	0	26	theme	consumption	21:31	arg1	Effects					0:6	Effects	0:6	Effects of long-term consumption of sucralose	0:44	Effects of long-term consumption of sucralose associated with high-fat diet in male mice.					
34486007	7	27	theme	human	1301:1305	arg1	health					1307:1312	human health	1301:1312	human health	1301:1312	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	4	28	theme	pancreatic	550:559	arg1	production					569:578	intestinal and pancreatic hormone production	535:578	intestinal and pancreatic hormone production	535:578	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	0	29	theme	long-term	11:19	arg1	consumption					21:31	long-term consumption	11:31	long-term consumption of sucralose	11:44	Effects of long-term consumption of sucralose associated with high-fat diet in male mice.					
34486007	6	30	theme	mice	852:855	arg1	evaluation					834:843	The evaluation	830:843	The evaluation of HFD mice	830:855	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	7	31	theme	future	1223:1228	arg1	research					1239:1246	future clinical research	1223:1246	future clinical research aimed at clarifying the impact of NNS consumption on human health	1223:1312	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	3	32	theme	high-fat	423:430	arg1	HFD					438:440	HFD	438:440	HFD	438:440	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	3	32	theme	high-fat	423:430	arg1	diet					432:435	high-fat diet	423:435	high-fat diet (HFD)	423:441	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	3	33	theme	libitum	366:372	arg1	consumption					384:394	ad libitum sucralose consumption	363:394	ad libitum sucralose consumption	363:394	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	6	34	theme	HFD	848:850	arg1	mice					852:855	HFD mice	848:855	HFD mice	848:855	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	6	35	theme	hepatic	945:951	arg1	steatosis					953:961	hepatic steatosis	945:961	hepatic steatosis	945:961	The evaluation of HFD mice showed that sucralose supplementation resulted in improvements in glycemic homeostasis, hepatic steatosis, and increased energy expenditure.					
34486007	7	36	theme	consumption	1286:1296	arg1	impact					1272:1277	the impact	1268:1277	the impact of NNS consumption on human health	1268:1312	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	5	37	theme	HFD-fed	756:762	arg1	mice					764:767	HFD-fed mice	756:767	HFD-fed mice	756:767	We observed that sucralose supplementation reduced weight gain in HFD-fed mice but did not change weight gain in mice fed with normal diet.					
34486007	3	38	theme	sucralose	374:382	arg1	consumption					384:394	ad libitum sucralose consumption	363:394	ad libitum sucralose consumption	363:394	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	4	39	theme	final	491:495	arg1	composition					502:512	final body composition	491:512	final body composition	491:512	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	4	40	theme	hormone	561:567	arg1	production					569:578	intestinal and pancreatic hormone production	535:578	intestinal and pancreatic hormone production	535:578	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	7	41	theme	different	1054:1062	arg1	outcomes					1064:1071	different outcomes	1054:1071	different outcomes in relation to weight gain	1054:1098	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	5	42	theme	weight	788:793	arg1	gain					795:798	weight gain	788:798	weight gain in mice fed with normal diet	788:827	We observed that sucralose supplementation reduced weight gain in HFD-fed mice but did not change weight gain in mice fed with normal diet.					
34486007	2	43	theme	weight	195:200	arg1	gain					202:205	weight gain	195:205	weight gain	195:205	Studies have shown that some NNS can favor weight gain by altering the intestinal microbiota, satiety hormone production, or aspects related to glucose homeostasis.					
34486007	3	44	theme	ad	363:364	arg1	consumption					384:394	ad libitum sucralose consumption	363:394	ad libitum sucralose consumption	363:394	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	7	45	from	impact	1272:1277	arg1	health					1307:1312	human health	1301:1312	human health	1301:1312	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	7	46	theme	NNS	1282:1284	arg1	consumption					1286:1296	NNS consumption	1282:1296	NNS consumption	1282:1296	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
34486007	1	47	theme	consumed	112:119	arg1	Sucralose					90:98	Sucralose	90:98	Sucralose	90:98	Sucralose is a widely consumed non-nutritive sweetener (NNS).					
34486007	1	47	theme	consumed	112:119	arg1	NNS					146:148	NNS	146:148	NNS	146:148	Sucralose is a widely consumed non-nutritive sweetener (NNS).					
34486007	1	47	theme	consumed	112:119	arg1	sweetener					135:143	a widely consumed non-nutritive sweetener	103:143	a widely consumed non-nutritive sweetener (NNS)	103:149	Sucralose is a widely consumed non-nutritive sweetener (NNS).					
34486007	2	48	theme	intestinal	223:232	arg1	microbiota					234:243	the intestinal microbiota	219:243	the intestinal microbiota	219:243	Studies have shown that some NNS can favor weight gain by altering the intestinal microbiota, satiety hormone production, or aspects related to glucose homeostasis.					
34486007	1	49	theme	non-nutritive	121:133	arg1	Sucralose					90:98	Sucralose	90:98	Sucralose	90:98	Sucralose is a widely consumed non-nutritive sweetener (NNS).					
34486007	1	49	theme	non-nutritive	121:133	arg1	NNS					146:148	NNS	146:148	NNS	146:148	Sucralose is a widely consumed non-nutritive sweetener (NNS).					
34486007	1	49	theme	non-nutritive	121:133	arg1	sweetener					135:143	a widely consumed non-nutritive sweetener	103:143	a widely consumed non-nutritive sweetener (NNS)	103:149	Sucralose is a widely consumed non-nutritive sweetener (NNS).					
34486007	4	50	theme	energy	515:520	arg1	expenditure					522:532	energy expenditure	515:532	energy expenditure	515:532	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	3	51	theme	extended	450:457	arg1	weeks					470:474	16 weeks	467:474	16 weeks	467:474	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	3	51	theme	extended	450:457	arg1	period					459:464	an extended period	447:464	an extended period (16 weeks)	447:475	In this study, we investigated the effects of ad libitum sucralose consumption in mice fed with normal or high-fat diet (HFD) for an extended period (16 weeks).					
34486007	4	52	theme	intestinal	535:544	arg1	production					569:578	intestinal and pancreatic hormone production	535:578	intestinal and pancreatic hormone production	535:578	Weight gain, final body composition, energy expenditure, intestinal and pancreatic hormone production, and endotoxemia during a voracity test, as well as liver and skeletal muscles were evaluated after 16 weeks.					
34486007	0	53	theme	high-fat	62:69	arg1	diet					71:74	high-fat diet	62:74	high-fat diet	62:74	Effects of long-term consumption of sucralose associated with high-fat diet in male mice.					
34486007	5	54	theme	weight	741:746	arg1	gain					748:751	weight gain	741:751	weight gain	741:751	We observed that sucralose supplementation reduced weight gain in HFD-fed mice but did not change weight gain in mice fed with normal diet.					
34486007	7	55	theme	weight	1088:1093	arg1	gain					1095:1098	weight gain	1088:1098	weight gain	1088:1098	Our results suggest that sucralose consumption promotes different outcomes in relation to weight gain when combined with different diets, which may explain the controversial data in previous studies, and can be considered in future clinical research aimed at clarifying the impact of NNS consumption on human health.					
32135230	0	0	theme	external	70:77	arg1	vibration					91:99	external lower punch vibration	70:99	external lower punch vibration	70:99	A novel approach to avoid capping and/or lamination by application of external lower punch vibration.					
32135230	9	1	theme	vibration	1212:1220	arg1	application					1166:1176	the application	1162:1176	the application of externally applied lower punch vibration	1162:1220	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	9	2	theme	tablets	1343:1349	arg1	stability					1313:1321	mechanical stability	1302:1321	mechanical stability of the manufactured tablets	1302:1349	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	8	3	theme	powder	1070:1075	arg1	properties					1077:1086	the physical powder properties	1057:1086	the physical powder properties	1057:1086	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	1	4	theme	common	140:145	arg1	problems					147:154	two common problems	136:154	two common problems	136:154	Capping as well as lamination are two common problems, which affect the resulting product quality of the tablet.					
32135230	1	4	theme	common	140:145	arg1	Capping					102:108	Capping	102:108	Capping as well as lamination	102:130	Capping as well as lamination are two common problems, which affect the resulting product quality of the tablet.					
32135230	1	4	theme	common	140:145	arg1	lamination					121:130	lamination	121:130	Capping as well as lamination	102:130	Capping as well as lamination are two common problems, which affect the resulting product quality of the tablet.					
32135230	4	5	theme	vibration	513:521	arg1	technique					523:531	a novel lower punch vibration technique	493:531	a novel lower punch vibration technique	493:531	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	0	6	theme	punch	85:89	arg1	vibration					91:99	external lower punch vibration	70:99	external lower punch vibration	70:99	A novel approach to avoid capping and/or lamination by application of external lower punch vibration.					
32135230	9	7	dep	capping	1258:1264	arg1	the					1254:1256	the	1254:1256	the	1254:1256	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	9	7	dep	capping	1258:1264	arg1	tendency					1280:1287	tendency	1280:1287	tendency	1280:1287	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	7	8	theme	tablet	911:916	arg1	weight					918:923	their tablet weight	905:923	their tablet weight	905:923	Moreover, the manufactured tablets were characterized regarding their tablet weight, tensile strength, and capping or lamination indices.					
32135230	6	9	theme	surface	827:833	arg1	area					835:838	surface area	827:838	surface area	827:838	The powders were investigated regarding their powder flow, density, particle morphology, and surface area.					
32135230	9	10	theme	pronounced	1231:1240	arg1	decrease					1242:1249	a pronounced decrease	1229:1249	a pronounced decrease of the capping or lamination tendency	1229:1287	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	3	11	theme	urgent	389:394	arg1	need					396:399	an urgent need	386:399	an urgent need for approaches to overcome the occurrences of capping and lamination	386:468	In this context, there is an urgent need for approaches to overcome the occurrences of capping and lamination.					
32135230	0	12	theme	vibration	91:99	arg1	application					55:65	application	55:65	application of external lower punch vibration	55:99	A novel approach to avoid capping and/or lamination by application of external lower punch vibration.					
32135230	4	13	theme	different	592:600	arg1	formulations					609:620	different powder formulations	592:620	different powder formulations	592:620	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	0	14	theme	novel	2:6	arg1	approach					8:15	A novel approach	0:15	A novel approach	0:15	A novel approach to avoid capping and/or lamination by application of external lower punch vibration.					
32135230	9	15	theme	lamination	1269:1278	arg1	decrease					1242:1249	a pronounced decrease	1229:1249	a pronounced decrease of the capping or lamination tendency	1229:1287	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	0	16	dep	external	70:77	arg1	lower					79:83	lower	79:83	lower	79:83	A novel approach to avoid capping and/or lamination by application of external lower punch vibration.					
32135230	5	17	theme	microcrystalline	633:648	arg1	powders					725:731	model powders	719:731	model powders	719:731	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	17	theme	microcrystalline	633:648	arg1	types					660:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	17	theme	microcrystalline	633:648	arg1	API					681:683	an API	678:683	an API (acetaminophen)	678:699	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	4	18	theme	novel	495:499	arg1	technique					523:531	a novel lower punch vibration technique	493:531	a novel lower punch vibration technique	493:531	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	4	19	theme	formulations	609:620	arg1	lamination					569:578	lamination	569:578	lamination	569:578	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	4	19	theme	formulations	609:620	arg1	capping					558:564	capping	558:564	capping	558:564	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	8	20	theme	adjusted	1126:1133	arg1	speed					1142:1146	the adjusted turret speed	1122:1146	the adjusted turret speed	1122:1146	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	9	21	theme	capping	1258:1264	arg1	decrease					1242:1249	a pronounced decrease	1229:1249	a pronounced decrease of the capping or lamination tendency	1229:1287	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	7	22	theme	manufactured	855:866	arg1	tablets					868:874	the manufactured tablets	851:874	the manufactured tablets	851:874	Moreover, the manufactured tablets were characterized regarding their tablet weight, tensile strength, and capping or lamination indices.					
32135230	2	23	theme	tablet	269:274	arg1	manufacturing					276:288	tablet manufacturing	269:288	tablet manufacturing	269:288	Usually, capping and lamination occur during or after tablet manufacturing, and may therefore influence follow-up processes such as the coating.					
32135230	4	24	theme	punch	507:511	arg1	technique					523:531	a novel lower punch vibration technique	493:531	a novel lower punch vibration technique	493:531	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	8	25	theme	capping	1001:1007	arg1	tendency					1023:1030	the capping or lamination tendency	997:1030	tendency	1023:1030	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	6	26	theme	particle	802:809	arg1	morphology					811:820	particle morphology	802:820	particle morphology	802:820	The powders were investigated regarding their powder flow, density, particle morphology, and surface area.					
32135230	2	27	theme	follow-up	319:327	arg1	processes					329:337	follow-up processes	319:337	follow-up processes such as the coating	319:357	Usually, capping and lamination occur during or after tablet manufacturing, and may therefore influence follow-up processes such as the coating.					
32135230	2	27	theme	follow-up	319:327	arg1	coating					351:357	the coating	347:357	the coating	347:357	Usually, capping and lamination occur during or after tablet manufacturing, and may therefore influence follow-up processes such as the coating.					
32135230	1	28	theme	resulting	174:182	arg1	quality					192:198	the resulting product quality	170:198	the resulting product quality of the tablet	170:212	Capping as well as lamination are two common problems, which affect the resulting product quality of the tablet.					
32135230	4	29	theme	lower	501:505	arg1	technique					523:531	a novel lower punch vibration technique	493:531	a novel lower punch vibration technique	493:531	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	1	30	theme	product	184:190	arg1	quality					192:198	the resulting product quality	170:198	the resulting product quality of the tablet	170:212	Capping as well as lamination are two common problems, which affect the resulting product quality of the tablet.					
32135230	4	31	theme	powder	602:607	arg1	formulations					609:620	different powder formulations	592:620	different powder formulations	592:620	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	4	32	theme	present	478:484	arg1	study					486:490	the present study	474:490	the present study	474:490	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	5	33	theme	model	719:723	arg1	powders					725:731	model powders	719:731	model powders	719:731	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	33	theme	model	719:723	arg1	types					660:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	33	theme	model	719:723	arg1	API					681:683	an API	678:683	an API (acetaminophen)	678:699	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	7	34	theme	capping	948:954	arg1	indices					970:976	capping or lamination indices	948:976	capping or lamination indices	948:976	Moreover, the manufactured tablets were characterized regarding their tablet weight, tensile strength, and capping or lamination indices.					
32135230	8	35	theme	formulation	1093:1103	arg1	composition					1105:1115	the formulation composition	1089:1115	the formulation composition	1089:1115	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	5	36	theme	Different	623:631	arg1	powders					725:731	model powders	719:731	model powders	719:731	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	36	theme	Different	623:631	arg1	types					660:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	36	theme	Different	623:631	arg1	API					681:683	an API	678:683	an API (acetaminophen)	678:699	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	7	37	theme	lamination	959:968	arg1	indices					970:976	capping or lamination indices	948:976	capping or lamination indices	948:976	Moreover, the manufactured tablets were characterized regarding their tablet weight, tensile strength, and capping or lamination indices.					
32135230	8	38	theme	lamination	1012:1021	arg1	tendency					1023:1030	the capping or lamination tendency	997:1030	tendency	1023:1030	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	4	39	used	used	537:540	arg2	technique					523:531	a novel lower punch vibration technique	493:531	a novel lower punch vibration technique	493:531	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	4	40	dep	capping	558:564	arg1	tendency					580:587	tendency	580:587	tendency	580:587	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	4	40	dep	capping	558:564	arg1	the					554:556	the	554:556	the	554:556	In the present study, a novel lower punch vibration technique was used to decrease the capping or lamination tendency of different powder formulations.					
32135230	3	41	theme	lamination	459:468	arg1	occurrences					432:442	the occurrences	428:442	the occurrences of capping and lamination	428:468	In this context, there is an urgent need for approaches to overcome the occurrences of capping and lamination.					
32135230	3	42	theme	capping	447:453	arg1	occurrences					432:442	the occurrences	428:442	the occurrences of capping and lamination	428:468	In this context, there is an urgent need for approaches to overcome the occurrences of capping and lamination.					
32135230	9	43	theme	mechanical	1302:1311	arg1	stability					1313:1321	mechanical stability	1302:1321	mechanical stability of the manufactured tablets	1302:1349	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	5	44	theme	cellulose	650:658	arg1	powders					725:731	model powders	719:731	model powders	719:731	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	44	theme	cellulose	650:658	arg1	types					660:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types	623:664	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	5	44	theme	cellulose	650:658	arg1	API					681:683	an API	678:683	an API (acetaminophen)	678:699	Different microcrystalline cellulose types, as well as an API (acetaminophen), were selected as model powders.					
32135230	6	45	theme	powder	780:785	arg1	flow					787:790	their powder flow	774:790	their powder flow	774:790	The powders were investigated regarding their powder flow, density, particle morphology, and surface area.					
32135230	9	46	theme	applied	1192:1198	arg1	vibration					1212:1220	externally applied lower punch vibration	1181:1220	externally applied lower punch vibration	1181:1220	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	7	47	theme	tensile	926:932	arg1	strength					934:941	tensile strength	926:941	tensile strength	926:941	Moreover, the manufactured tablets were characterized regarding their tablet weight, tensile strength, and capping or lamination indices.					
32135230	8	48	theme	turret	1135:1140	arg1	speed					1142:1146	the adjusted turret speed	1122:1146	the adjusted turret speed	1122:1146	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	8	49	theme	physical	1061:1068	arg1	properties					1077:1086	the physical powder properties	1057:1086	the physical powder properties	1057:1086	It was shown that the capping or lamination tendency was strongly affected by the physical powder properties, the formulation composition, and the adjusted turret speed.					
32135230	1	50	theme	tablet	207:212	arg1	quality					192:198	the resulting product quality	170:198	the resulting product quality of the tablet	170:212	Capping as well as lamination are two common problems, which affect the resulting product quality of the tablet.					
32135230	9	51	theme	punch	1206:1210	arg1	vibration					1212:1220	externally applied lower punch vibration	1181:1220	externally applied lower punch vibration	1181:1220	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
32135230	9	52	theme	manufactured	1330:1341	arg1	tablets					1343:1349	the manufactured tablets	1326:1349	the manufactured tablets	1326:1349	In addition, the application of externally applied lower punch vibration led to a pronounced decrease of the capping or lamination tendency and improved mechanical stability of the manufactured tablets.					
34516546	3	0	theme	Various	351:357	arg1	methods					373:379	Various pre-treatment methods	351:379	Various pre-treatment methods	351:379	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	10	1	theme	enzymes	1322:1328	arg1	action					1312:1317	the synergistic action	1296:1317	the synergistic action of enzymes	1296:1328	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	3	2	theme	pre-treatment	359:371	arg1	methods					373:379	Various pre-treatment methods	351:379	Various pre-treatment methods	351:379	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	4	3	theme	computational	558:570	arg1	model					572:576	a computational model	556:576	a computational model that complements the experimental approaches	556:621	In this study, we present a computational model that complements the experimental approaches.					
34516546	1	4	theme	promising	177:185	arg1	alternative					187:197	a promising alternative	175:197	a promising alternative to conventional biofuel production	175:232	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	1	4	theme	promising	177:185	arg1	processing					67:76	The processing	63:76	The processing of agricultural wastes towards extraction of renewable resources	63:141	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	7	5	theme	enzymes	964:970	arg1	cocktails					932:940	different cocktails	922:940	different cocktails of well characterized enzymes	922:970	This substrate is subjected to digestion by different cocktails of well characterized enzymes.					
34516546	11	6	theme	substrate	1544:1552	arg1	composition					1554:1564	the substrate composition	1540:1564	the substrate composition	1540:1564	Importantly, we show that considering the crystallinity of cellulose in addition to the substrate composition is essential to interpret experimental saccharification data.					
34516546	8	7	from	simulated	1006:1014	arg1	silico					1019:1024	silico	1019:1024	silico using a stochastic procedure based on a Gillespie algorithm	1019:1084	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	8	8	theme	Gillespie	1066:1074	arg1	algorithm					1076:1084	a Gillespie algorithm	1064:1084	a Gillespie algorithm	1064:1084	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	10	9	theme	linear	1348:1353	arg1	decrease					1355:1362	the linear decrease	1344:1362	the linear decrease of sugar conversion	1344:1382	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	9	10	theme	saccharification	1228:1243	arg1	courses					1250:1256	experimental saccharification time courses	1215:1256	experimental saccharification time courses for corn stover	1215:1272	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	9	11	theme	runs	1184:1187	arg1	parameters					1155:1164	the parameters	1151:1164	the parameters of the simulation runs	1151:1187	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	9	12	theme	simulation	1173:1182	arg1	runs					1184:1187	the simulation runs	1169:1187	the simulation runs	1169:1187	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	10	13	theme	synergistic	1300:1310	arg1	action					1312:1317	the synergistic action	1296:1317	the synergistic action of enzymes	1296:1328	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	6	14	dep	microfibril	749:759	arg1	composed					761:768	composed	761:768	composed of cellulose	761:781	We model a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin, with various relative abundances and arrangements.					
34516546	6	14	dep	microfibril	749:759	arg1	surrounded					787:796	surrounded	787:796	surrounded by hemicellulose and lignin	787:824	We model a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin, with various relative abundances and arrangements.					
34516546	10	15	theme	lignin	1396:1401	arg1	content					1403:1409	lignin content	1396:1409	lignin content	1396:1409	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	5	16	theme	substrate	679:687	arg1	structure					662:670	the three-dimensional structure	640:670	the three-dimensional structure of the substrate	640:687	We decipher how the three-dimensional structure of the substrate impacts the saccharification dynamics.					
34516546	1	17	theme	agricultural	81:92	arg1	wastes					94:99	agricultural wastes	81:99	agricultural wastes	81:99	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	1	18	theme	conventional	202:213	arg1	production					223:232	conventional biofuel production	202:232	conventional biofuel production	202:232	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	11	19	theme	cellulose	1515:1523	arg1	crystallinity					1498:1510	the crystallinity	1494:1510	the crystallinity of cellulose	1494:1523	Importantly, we show that considering the crystallinity of cellulose in addition to the substrate composition is essential to interpret experimental saccharification data.					
34516546	10	20	theme	substrate	1435:1443	arg1	crystallinity					1414:1426	crystallinity	1414:1426	crystallinity	1414:1426	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	10	20	theme	substrate	1435:1443	arg1	content					1403:1409	lignin content	1396:1409	lignin content	1396:1409	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	1	21	theme	wastes	94:99	arg1	processing					67:76	The processing	63:76	The processing of agricultural wastes towards extraction of renewable resources	63:141	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	1	21	theme	wastes	94:99	arg1	alternative					187:197	a promising alternative	175:197	a promising alternative to conventional biofuel production	175:232	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	8	22	theme	saccharification	977:992	arg1	simulated					1006:1014	simulated	1006:1014	simulated	1006:1014	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	8	22	theme	saccharification	977:992	arg1	dynamics					994:1001	The saccharification dynamics	973:1001	The saccharification dynamics	973:1001	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	5	23	theme	saccharification	701:716	arg1	dynamics					718:725	the saccharification dynamics	697:725	the saccharification dynamics	697:725	We decipher how the three-dimensional structure of the substrate impacts the saccharification dynamics.					
34516546	10	24	theme	conversion	1373:1382	arg1	decrease					1355:1362	the linear decrease	1344:1362	the linear decrease of sugar conversion	1344:1382	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
34516546	3	25	theme	main	469:472	arg1	obstacles					474:482	the main obstacles	465:482	the main obstacles in increasing the enzymatic saccharification	465:527	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	0	26	theme	Stochastic	0:9	arg1	model					11:15	Stochastic model	0:15	Stochastic model of lignocellulosic material	0:43	Stochastic model of lignocellulosic material saccharification.					
34516546	1	27	theme	biofuel	215:221	arg1	production					223:232	conventional biofuel production	202:232	conventional biofuel production	202:232	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	0	28	theme	lignocellulosic	20:34	arg1	material					36:43	lignocellulosic material	20:43	lignocellulosic material	20:43	Stochastic model of lignocellulosic material saccharification.					
34516546	4	29	theme	experimental	599:610	arg1	approaches					612:621	the experimental approaches	595:621	the experimental approaches	595:621	In this study, we present a computational model that complements the experimental approaches.					
34516546	5	30	theme	three-dimensional	644:660	arg1	structure					662:670	the three-dimensional structure	640:670	the three-dimensional structure of the substrate	640:687	We decipher how the three-dimensional structure of the substrate impacts the saccharification dynamics.					
34516546	7	31	theme	different	922:930	arg1	cocktails					932:940	different cocktails	922:940	different cocktails of well characterized enzymes	922:970	This substrate is subjected to digestion by different cocktails of well characterized enzymes.					
34516546	3	32	theme	subsequent	421:430	arg1	modification					432:443	the subsequent modification	417:443	the subsequent modification of the material	417:459	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	9	33	theme	time	1245:1248	arg1	courses					1250:1256	experimental saccharification time courses	1215:1256	experimental saccharification time courses for corn stover	1215:1272	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	1	34	theme	renewable	123:131	arg1	resources					133:141	renewable resources	123:141	renewable resources	123:141	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	6	35	theme	wall	744:747	arg1	microfibril					749:759	a cell wall microfibril	737:759	a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin	737:824	We model a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin, with various relative abundances and arrangements.					
34516546	7	36	theme	characterized	950:962	arg1	enzymes					964:970	well characterized enzymes	945:970	well characterized enzymes	945:970	This substrate is subjected to digestion by different cocktails of well characterized enzymes.					
34516546	6	37	theme	cell	739:742	arg1	microfibril					749:759	a cell wall microfibril	737:759	a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin	737:824	We model a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin, with various relative abundances and arrangements.					
34516546	0	38	theme	material	36:43	arg1	model					11:15	Stochastic model	0:15	Stochastic model of lignocellulosic material	0:43	Stochastic model of lignocellulosic material saccharification.					
34516546	8	39	from	silico	1019:1024	arg1	simulated					1006:1014	simulated	1006:1014	simulated	1006:1014	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	8	39	from	silico	1019:1024	arg1	dynamics					994:1001	The saccharification dynamics	973:1001	The saccharification dynamics	973:1001	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	9	40	theme	experimental	1215:1226	arg1	courses					1250:1256	experimental saccharification time courses	1215:1256	experimental saccharification time courses for corn stover	1215:1272	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	2	41	theme	residues	267:274	arg1	process					298:304	a complex chemical process	279:304	a complex chemical process that is currently time intensive and costly	279:348	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	2	41	theme	residues	267:274	arg1	intensive					329:337	intensive	329:337	intensive	329:337	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	2	41	theme	residues	267:274	arg1	degradation					239:249	The degradation	235:249	The degradation of agricultural residues	235:274	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	6	42	theme	relative	840:847	arg1	abundances					849:858	various relative abundances	832:858	various relative abundances	832:858	We model a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin, with various relative abundances and arrangements.					
34516546	3	43	theme	enzymatic	502:510	arg1	saccharification					512:527	the enzymatic saccharification	498:527	the enzymatic saccharification	498:527	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	2	44	theme	agricultural	254:265	arg1	residues					267:274	agricultural residues	254:274	agricultural residues	254:274	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	6	45	theme	various	832:838	arg1	abundances					849:858	various relative abundances	832:858	various relative abundances	832:858	We model a cell wall microfibril composed of cellulose and surrounded by hemicellulose and lignin, with various relative abundances and arrangements.					
34516546	1	46	theme	resources	133:141	arg1	extraction					109:118	extraction	109:118	extraction of renewable resources	109:141	The processing of agricultural wastes towards extraction of renewable resources is recently being considered as a promising alternative to conventional biofuel production.					
34516546	3	47	theme	material	452:459	arg1	modification					432:443	the subsequent modification	417:443	the subsequent modification of the material	417:459	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	3	47	theme	material	452:459	arg1	obstacles					474:482	the main obstacles	465:482	the main obstacles in increasing the enzymatic saccharification	465:527	Various pre-treatment methods are being investigated to determine the subsequent modification of the material and the main obstacles in increasing the enzymatic saccharification.					
34516546	8	48	theme	stochastic	1034:1043	arg1	procedure					1045:1053	a stochastic procedure	1032:1053	a stochastic procedure based on a Gillespie algorithm	1032:1084	The saccharification dynamics is simulated in silico using a stochastic procedure based on a Gillespie algorithm.					
34516546	4	49	attach	present	548:554	arg1	study					538:542	this study	533:542	this study	533:542	In this study, we present a computational model that complements the experimental approaches.					
34516546	4	49	attach	present	548:554	arg2	we					545:546	we	545:546	we	545:546	In this study, we present a computational model that complements the experimental approaches.					
34516546	9	50	theme	fitting	1118:1124	arg1	procedure					1126:1134	a fitting procedure	1116:1134	a fitting procedure that optimizes the parameters of the simulation runs	1116:1187	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	2	51	theme	chemical	289:296	arg1	degradation					239:249	The degradation	235:249	The degradation of agricultural residues	235:274	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	2	51	theme	chemical	289:296	arg1	intensive					329:337	intensive	329:337	intensive	329:337	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	2	51	theme	chemical	289:296	arg1	process					298:304	a complex chemical process	279:304	a complex chemical process that is currently time intensive and costly	279:348	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	11	52	theme	experimental	1592:1603	arg1	data					1622:1625	experimental saccharification data	1592:1625	experimental saccharification data	1592:1625	Importantly, we show that considering the crystallinity of cellulose in addition to the substrate composition is essential to interpret experimental saccharification data.					
34516546	12	53	theme	xylan	1676:1680	arg1	hypothesis					1662:1671	the hypothesis	1658:1671	the hypothesis of xylan	1658:1680	Finally, our findings support the hypothesis of xylan being partially crystalline.					
34516546	2	54	theme	complex	281:287	arg1	degradation					239:249	The degradation	235:249	The degradation of agricultural residues	235:274	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	2	54	theme	complex	281:287	arg1	intensive					329:337	intensive	329:337	intensive	329:337	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	2	54	theme	complex	281:287	arg1	process					298:304	a complex chemical process	279:304	a complex chemical process that is currently time intensive and costly	279:348	The degradation of agricultural residues is a complex chemical process that is currently time intensive and costly.					
34516546	9	55	theme	corn	1262:1265	arg1	stover					1267:1272	corn stover	1262:1272	corn stover	1262:1272	As we additionally implement a fitting procedure that optimizes the parameters of the simulation runs, we are able to reproduce experimental saccharification time courses for corn stover.					
34516546	11	56	theme	saccharification	1605:1620	arg1	data					1622:1625	experimental saccharification data	1592:1625	experimental saccharification data	1592:1625	Importantly, we show that considering the crystallinity of cellulose in addition to the substrate composition is essential to interpret experimental saccharification data.					
34516546	10	57	theme	sugar	1367:1371	arg1	conversion					1373:1382	sugar conversion	1367:1382	sugar conversion	1367:1382	Our model highlights the synergistic action of enzymes, and confirms the linear decrease of sugar conversion when either lignin content or crystallinity of the substrate increases.					
32083322	6	0	with	analysis	1110:1117	arg1	leucocephala					1143:1154	Leucaena (Leucaena leucocephala	1124:1154	Leucaena (Leucaena leucocephala	1124:1154	The analysis with Leucaena (Leucaena leucocephala Lam.					
32083322	1	1	theme	feedstuff	317:325	arg1	ingredients					327:337	feedstuff ingredients	317:337	feedstuff ingredients	317:337	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	2	2	dep	starch	455:460	arg1	St					463:464	St	463:464	St	463:464	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	9	3	dep	In	1512:1513	arg1	vitro					1515:1519	vitro	1515:1519	vitro	1515:1519	In vitro GP could be used to quantify the components of some forages, although further studies are necessary.					
32083322	1	4	theme	gas	154:156	arg1	technique					174:182	The in vitro gas production (GP) technique	141:182	The in vitro gas production (GP) technique	141:182	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	1	4	theme	gas	154:156	arg1	useful					193:198	useful	193:198	useful	193:198	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	3	5	theme	Cel	705:707	arg1	St					698:699	St	698:699	St	698:699	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	5	theme	Cel	705:707	arg1	glucose					683:689	glucose	683:689	glucose (Glu)	683:695	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	5	theme	Cel	705:707	arg1	0.02					646:649	0.02	646:649	0.02	646:649	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	5	theme	Cel	705:707	arg1	Cel					705:707	Cel	705:707	Cel	705:707	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	5	theme	Cel	705:707	arg1	Glu					692:694	Glu	692:694	Glu	692:694	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	6	6	theme	Leucaena	1134:1141	arg1	leucocephala					1143:1154	Leucaena (Leucaena leucocephala	1124:1154	Leucaena (Leucaena leucocephala	1124:1154	The analysis with Leucaena (Leucaena leucocephala Lam.					
32083322	0	7	theme	in	95:96	arg1	technique					119:127	an in vitro gas production technique	92:127	an in vitro gas production technique	92:127	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	1	8	theme	ingredients	327:337	arg1	components					303:312	the components	299:312	the components of feedstuff ingredients	299:337	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	2	9	dep	RESULTS	340:346	arg1	validated					381:389	validated	381:389	validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP	381:516	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	2	9	dep	RESULTS	340:346	arg1	generated					367:375	generated	367:375	generated	367:375	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	1	10	theme	feedstuffs	246:255	arg1	degradability					229:241	the potential degradability	215:241	the potential degradability of feedstuffs in ruminal environments	215:279	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	9	11	theme	further	1591:1597	arg1	studies					1599:1605	further studies	1591:1605	further studies	1591:1605	In vitro GP could be used to quantify the components of some forages, although further studies are necessary.					
32083322	5	12	theme	low	989:991	arg1	LF48					1017:1020	LF48	1017:1020	LF48; equivalent to Cel	1017:1039	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	12	theme	low	989:991	arg1	fractions					1006:1014	low fermentation fractions	989:1014	low fermentation fractions (LF48; equivalent to Cel)	989:1040	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	1	13	dep	BACKGROUND	130:139	arg1	technique					174:182	The in vitro gas production (GP) technique	141:182	The in vitro gas production (GP) technique	141:182	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	1	13	dep	BACKGROUND	130:139	arg1	useful					193:198	useful	193:198	useful	193:198	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	5	14	from	0.935	827:831	arg1	r2					810:811	r2	810:811	r2 from 0.709 to 0.935	810:831	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	15	theme	glucose	683:689	arg1	St					698:699	St	698:699	St	698:699	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	15	theme	glucose	683:689	arg1	glucose					683:689	glucose	683:689	glucose (Glu)	683:695	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	15	theme	glucose	683:689	arg1	0.02					646:649	0.02	646:649	0.02	646:649	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	15	theme	glucose	683:689	arg1	Cel					705:707	Cel	705:707	Cel	705:707	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	15	theme	glucose	683:689	arg1	Glu					692:694	Glu	692:694	Glu	692:694	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	0	16	theme	gas	104:106	arg1	technique					119:127	an in vitro gas production technique	92:127	an in vitro gas production technique	92:127	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	5	17	theme	rapid	878:882	arg1	RF					908:909	RF	908:909	RF; equivalent to Glu	908:928	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	17	theme	rapid	878:882	arg1	fractions					897:905	rapid fermentation fractions	878:905	rapid fermentation fractions (RF; equivalent to Glu)	878:929	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	0	18	dep	in	95:96	arg1	vitro					98:102	vitro	98:102	vitro	98:102	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	5	19	theme	RF	1050:1051	arg1	models					1064:1069	CS, RF, MF and LF models	1046:1069	CS, RF, MF and LF models	1046:1069	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	1	20	theme	production	158:167	arg1	technique					174:182	The in vitro gas production (GP) technique	141:182	The in vitro gas production (GP) technique	141:182	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	1	20	theme	production	158:167	arg1	useful					193:198	useful	193:198	useful	193:198	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	5	21	theme	fermentation	884:895	arg1	RF					908:909	RF	908:909	RF; equivalent to Glu	908:928	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	21	theme	fermentation	884:895	arg1	fractions					897:905	rapid fermentation fractions	878:905	rapid fermentation fractions (RF; equivalent to Glu)	878:929	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	10	22	theme	Chemical	1640:1647	arg1	Industry					1649:1656	Chemical Industry	1640:1656	Chemical Industry	1640:1656	© 2020 Society of Chemical Industry.					
32083322	8	23	theme	fractions	1297:1305	arg1	GP					1275:1276	The in vitro GP	1262:1276	The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel	1262:1335	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	0	24	theme	non-fibrous	12:22	arg1	carbohydrates					24:36	non-fibrous carbohydrates	12:36	non-fibrous carbohydrates	12:36	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	5	25	theme	medium	932:937	arg1	MF					963:964	MF	963:964	MF; equivalent to St	963:982	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	25	theme	medium	932:937	arg1	fractions					952:960	medium fermentation fractions	932:960	medium fermentation fractions (MF; equivalent to St)	932:983	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	26	theme	models	532:537	arg1	Residuals					519:527	Residuals	519:527	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.	519:721	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	5	27	theme	MF	1054:1055	arg1	models					1064:1069	CS, RF, MF and LF models	1046:1069	CS, RF, MF and LF models	1046:1069	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	1	28	theme	GP	170:171	arg1	technique					174:182	The in vitro gas production (GP) technique	141:182	The in vitro gas production (GP) technique	141:182	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	1	28	theme	GP	170:171	arg1	useful					193:198	useful	193:198	useful	193:198	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	2	29	theme	fiber	448:452	arg1	Hem					482:484	Hem	482:484	Hem	482:484	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	2	29	theme	fiber	448:452	arg1	/hemicellulose					466:479	neutral detergent-soluble fiber, starch (St)/hemicellulose	422:479	/hemicellulose	466:479	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	1	30	theme	ruminal	260:266	arg1	environments					268:279	ruminal environments	260:279	ruminal environments	260:279	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	8	31	theme	ruminant	1496:1503	arg1	diets					1505:1509	ruminant diets	1496:1509	ruminant diets	1496:1509	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	8	32	dep	in	1266:1267	arg1	vitro					1269:1273	vitro	1269:1273	vitro	1269:1273	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	5	33	dep	0.935	827:831	arg1	to					824:825	to	824:825	to	824:825	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	2	34	theme	detergent-soluble	430:446	arg1	Hem					482:484	Hem	482:484	Hem	482:484	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	2	34	theme	detergent-soluble	430:446	arg1	/hemicellulose					466:479	neutral detergent-soluble fiber, starch (St)/hemicellulose	422:479	/hemicellulose	466:479	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	3	35	theme	0.02	646:649	arg1	GP-8					577:580	GP-8	577:580	GP-8	577:580	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	35	theme	0.02	646:649	arg1	h					574:574	GP [0-8 h	566:574	GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	566:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	8	36	theme	chemical	1393:1400	arg1	composition					1402:1412	the chemical composition	1389:1412	the chemical composition	1389:1412	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	2	37	theme	neutral	422:428	arg1	Hem					482:484	Hem	482:484	Hem	482:484	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	2	37	theme	neutral	422:428	arg1	/hemicellulose					466:479	neutral detergent-soluble fiber, starch (St)/hemicellulose	422:479	/hemicellulose	466:479	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	0	38	theme	production	108:117	arg1	technique					119:127	an in vitro gas production technique	92:127	an in vitro gas production technique	92:127	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	6	39	theme	Leucaena	1124:1131	arg1	leucocephala					1143:1154	Leucaena (Leucaena leucocephala	1124:1154	Leucaena (Leucaena leucocephala	1124:1154	The analysis with Leucaena (Leucaena leucocephala Lam.					
32083322	1	40	dep	in	145:146	arg1	vitro					148:152	vitro	148:152	vitro	148:152	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	9	41	theme	forages	1573:1579	arg1	components					1554:1563	the components	1550:1563	the components of some forages	1550:1579	In vitro GP could be used to quantify the components of some forages, although further studies are necessary.					
32083322	0	42	theme	detergent	44:52	arg1	fiber					54:58	acid detergent fiber and cellulose of	39:75	fiber	54:58	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	1	43	from	degradability	229:241	arg1	environments					268:279	ruminal environments	260:279	ruminal environments	260:279	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	8	44	theme	harvest	1366:1372	arg1	time					1374:1377	harvest time	1366:1377	harvest time	1366:1377	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	5	45	theme	CS	1046:1047	arg1	models					1064:1069	CS, RF, MF and LF models	1046:1069	CS, RF, MF and LF models	1046:1069	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	46	dep	h	574:574	arg1	> 24-81 h					624:632	> 24-81 h	624:632	> 24-81 h	624:632	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	46	dep	h	574:574	arg1	GP-81					635:639	GP-81	635:639	GP-81	635:639	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	46	dep	h	574:574	arg1	> 24-48 h					602:610	> 24-48 h	602:610	> 24-48 h	602:610	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	46	dep	h	574:574	arg1	> 8-24 h					584:591	> 8-24 h	584:591	> 8-24 h	584:591	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	46	dep	h	574:574	arg1	GP-48					613:617	GP-48	613:617	GP-48	613:617	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	46	dep	h	574:574	arg1	GP-24					594:598	GP-24	594:598	GP-24	594:598	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	0	47	theme	acid	39:42	arg1	fiber					54:58	acid detergent fiber and cellulose of	39:75	fiber	54:58	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	7	48	theme	star	1173:1176	arg1	grass					1178:1182	star grass	1173:1182	star grass (Cynodon nlemfuensis Vanderyst)	1173:1214	de Wit) and star grass (Cynodon nlemfuensis Vanderyst) consider high-protein ingredients.					
32083322	7	48	theme	star	1173:1176	arg1	Vanderyst					1205:1213	Vanderyst	1205:1213	Vanderyst	1205:1213	de Wit) and star grass (Cynodon nlemfuensis Vanderyst) consider high-protein ingredients.					
32083322	4	49	theme	Glu	768:770	arg1	Cel					780:782	Cel	780:782	Cel	780:782	The incubations were analyzed in mixtures of Glu, St and Cel.					
32083322	4	49	theme	Glu	768:770	arg1	St					773:774	St	773:774	St	773:774	The incubations were analyzed in mixtures of Glu, St and Cel.					
32083322	4	49	theme	Glu	768:770	arg1	mixtures					756:763	mixtures	756:763	mixtures of Glu	756:770	The incubations were analyzed in mixtures of Glu, St and Cel.					
32083322	7	50	theme	high-protein	1225:1236	arg1	ingredients					1238:1248	high-protein ingredients	1225:1248	high-protein ingredients	1225:1248	de Wit) and star grass (Cynodon nlemfuensis Vanderyst) consider high-protein ingredients.					
32083322	8	51	theme	equivalent	1307:1316	arg1	RF					1281:1282	RF	1281:1282	RF	1281:1282	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	5	52	theme	standardized	1075:1086	arg1	residuals < 0.09					1088:1103	standardized residuals < 0.09	1075:1103	standardized residuals < 0.09	1075:1103	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	53	theme	0.08	658:661	arg1	GP-8					577:580	GP-8	577:580	GP-8	577:580	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	53	theme	0.08	658:661	arg1	h					574:574	GP [0-8 h	566:574	GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	566:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	5	54	theme	fermentation	939:950	arg1	MF					963:964	MF	963:964	MF; equivalent to St	963:982	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	54	theme	fermentation	939:950	arg1	fractions					952:960	medium fermentation fractions	932:960	medium fermentation fractions (MF; equivalent to St)	932:983	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	7	55	theme	de	1161:1162	arg1	Wit					1164:1166	de Wit)	1161:1167	de Wit)	1161:1167	de Wit) and star grass (Cynodon nlemfuensis Vanderyst) consider high-protein ingredients.					
32083322	9	56	used	used	1533:1536	arg2	GP					1521:1522	In vitro GP	1512:1522	In vitro GP	1512:1522	In vitro GP could be used to quantify the components of some forages, although further studies are necessary.					
32083322	5	57	theme	equivalent	912:921	arg1	RF					908:909	RF	908:909	RF; equivalent to Glu	908:928	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	57	theme	equivalent	912:921	arg1	fractions					897:905	rapid fermentation fractions	878:905	rapid fermentation fractions (RF; equivalent to Glu)	878:929	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	8	58	theme	MF	1285:1286	arg1	GP					1275:1276	The in vitro GP	1262:1276	The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel	1262:1335	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	2	59	dep	/hemicellulose	466:479	arg1	starch					455:460	starch	455:460	starch	455:460	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	5	60	dep	models	802:807	arg1	r2					810:811	r2	810:811	r2 from 0.709 to 0.935	810:831	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	61	theme	St	698:699	arg1	St					698:699	St	698:699	St	698:699	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	61	theme	St	698:699	arg1	glucose					683:689	glucose	683:689	glucose (Glu)	683:695	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	61	theme	St	698:699	arg1	0.02					646:649	0.02	646:649	0.02	646:649	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	61	theme	St	698:699	arg1	Cel					705:707	Cel	705:707	Cel	705:707	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	61	theme	St	698:699	arg1	Glu					692:694	Glu	692:694	Glu	692:694	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	62	theme	0.04	652:655	arg1	GP-8					577:580	GP-8	577:580	GP-8	577:580	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	62	theme	0.04	652:655	arg1	h					574:574	GP [0-8 h	566:574	GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	566:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	8	63	theme	diets	1505:1509	arg1	design					1486:1491	the design	1482:1491	the design of ruminant diets	1482:1509	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	8	64	theme	in	1266:1267	arg1	GP					1275:1276	The in vitro GP	1262:1276	The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel	1262:1335	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	3	65	theme	h	574:574	arg1	peaks					557:561	the peaks	553:561	the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	553:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	5	66	theme	equivalent	1023:1032	arg1	LF48					1017:1020	LF48	1017:1020	LF48; equivalent to Cel	1017:1039	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	66	theme	equivalent	1023:1032	arg1	fractions					1006:1014	low fermentation fractions	989:1014	low fermentation fractions (LF48; equivalent to Cel)	989:1040	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	8	67	theme	LF48	1292:1295	arg1	fractions					1297:1305	LF48 fractions	1292:1305	LF48 fractions	1292:1305	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	6	68	dep	Lam	1156:1158	arg1	analysis					1110:1117	The analysis	1106:1117	The analysis with Leucaena (Leucaena leucocephala	1106:1154	The analysis with Leucaena (Leucaena leucocephala Lam.					
32083322	5	69	theme	corn	849:852	arg1	CS					862:863	CS	862:863	CS	862:863	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	69	theme	corn	849:852	arg1	stover					854:859	corn stover	849:859	corn stover (CS)	849:864	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	8	70	dep	CONCLUSION	1251:1260	arg1	considered					1464:1473	considered	1464:1473	should be considered during the design of ruminant diets	1454:1509	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	8	70	dep	CONCLUSION	1251:1260	arg1	affected					1341:1348	affected	1341:1348	are affected by maturity and harvest time even when the chemical composition remains similar	1337:1428	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	1	71	theme	in	145:146	arg1	technique					174:182	The in vitro gas production (GP) technique	141:182	The in vitro gas production (GP) technique	141:182	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	1	71	theme	in	145:146	arg1	useful					193:198	useful	193:198	useful	193:198	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32083322	9	72	theme	In	1512:1513	arg1	GP					1521:1522	In vitro GP	1512:1522	In vitro GP	1512:1522	In vitro GP could be used to quantify the components of some forages, although further studies are necessary.					
32083322	3	73	theme	0.20 g	673:678	arg1	GP-8					577:580	GP-8	577:580	GP-8	577:580	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	73	theme	0.20 g	673:678	arg1	h					574:574	GP [0-8 h	566:574	GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	566:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	0	74	theme	of	74:75	arg1	cellulose					64:72	acid detergent fiber and cellulose of	39:75	cellulose	64:72	Quantifying non-fibrous carbohydrates, acid detergent fiber and cellulose of forage through an in vitro gas production technique.					
32083322	5	75	theme	fitting	794:800	arg1	models					802:807	The best fitting models	785:807	The best fitting models (r2 from 0.709 to 0.935)	785:832	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	76	theme	GP	566:567	arg1	GP-8					577:580	GP-8	577:580	GP-8	577:580	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	76	theme	GP	566:567	arg1	h					574:574	GP [0-8 h	566:574	GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	566:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	5	77	theme	LF	1061:1062	arg1	models					1064:1069	CS, RF, MF and LF models	1046:1069	CS, RF, MF and LF models	1046:1069	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	8	78	theme	RF	1281:1282	arg1	GP					1275:1276	The in vitro GP	1262:1276	The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel	1262:1335	CONCLUSION The in vitro GP of RF, MF and LF48 fractions equivalent to Glu, St and Cel are affected by maturity and harvest time even when the chemical composition remains similar, and so RF, MF and LF48 should be considered during the design of ruminant diets.					
32083322	5	79	theme	equivalent	967:976	arg1	MF					963:964	MF	963:964	MF; equivalent to St	963:982	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	79	theme	equivalent	967:976	arg1	fractions					952:960	medium fermentation fractions	932:960	medium fermentation fractions (MF; equivalent to St)	932:983	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	3	80	theme	0.12	664:667	arg1	GP-8					577:580	GP-8	577:580	GP-8	577:580	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	3	80	theme	0.12	664:667	arg1	h					574:574	GP [0-8 h	566:574	GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively	566:720	Residuals of models obtained from the peaks of GP [0-8 h (GP-8), > 8-24 h (GP-24), > 24-48 h (GP-48) and > 24-81 h (GP-81)] of 0.02, 0.04, 0.08, 0.12 and 0.20 g of glucose (Glu), St and Cel respectively.					
32083322	2	81	theme	Linear	348:353	arg1	alternatives					394:405	alternatives	394:405	alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP	394:516	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	2	81	theme	Linear	348:353	arg1	models					355:360	Linear models	348:360	Linear models	348:360	RESULTS Linear models were generated and validated as alternatives of quantifying neutral detergent-soluble fiber, starch (St)/hemicellulose (Hem) and cellulose (Cel) through GP.					
32083322	5	82	theme	fermentation	993:1004	arg1	LF48					1017:1020	LF48	1017:1020	LF48; equivalent to Cel	1017:1039	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	5	82	theme	fermentation	993:1004	arg1	fractions					1006:1014	low fermentation fractions	989:1014	low fermentation fractions (LF48; equivalent to Cel)	989:1040	The best fitting models (r2 from 0.709 to 0.935) were tested on corn stover (CS) to quantify rapid fermentation fractions (RF; equivalent to Glu), medium fermentation fractions (MF; equivalent to St) and low fermentation fractions (LF48; equivalent to Cel); in CS, RF, MF and LF models had standardized residuals < 0.09.					
32083322	1	83	theme	potential	219:227	arg1	degradability					229:241	the potential degradability	215:241	the potential degradability of feedstuffs in ruminal environments	215:279	BACKGROUND The in vitro gas production (GP) technique has been useful for evaluating the potential degradability of feedstuffs in ruminal environments; GP is related to the components of feedstuff ingredients.					
32279736	0	0	from	frameworks	34:43	arg1	basis					52:56	the basis	48:56	the basis of γ-cyclodextrin	48:74	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	5	1	dep	media	828:832	arg1	FaSSIF					843:848	FaSSIF	843:848	FaSSIF	843:848	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	5	1	dep	media	828:832	arg1	FaSSGF					835:840	FaSSGF	835:840	FaSSGF	835:840	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	1	2	from	frameworks	167:176	arg1	basis					185:189	the basis	181:189	the basis of γ-cyclodextrin (γCD-MOF)	181:217	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	6	3	with	formation	909:917	arg1	γ-CD					931:934	γ-CD	931:934	γ-CD	931:934	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	1	4	used	used	284:287	arg2	methotrexate					249:260	methotrexate	249:260	methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant	249:339	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	1	4	used	used	284:287	arg2	agent					305:309	chemotherapy agent	292:309	chemotherapy agent	292:309	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	1	4	used	used	284:287	arg2	system					322:327	immune system	315:327	immune system	315:327	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	1	4	used	used	284:287	arg2	MTX					263:265	MTX	263:265	MTX	263:265	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	6	5	theme	MTX	922:924	arg1	formation					909:917	Inclusion complex formation	891:917	Inclusion complex formation of MTX with γ-CD	891:934	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	3	6	theme	N2	501:502	arg1	adsorption/desorption					504:524	N2 adsorption/desorption	501:524	N2 adsorption/desorption	501:524	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	0	7	theme	in	103:104	arg1	investigation					124:136	in vitro and in vivo investigation	103:136	in vitro and in vivo investigation	103:136	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	0	7	theme	in	103:104	arg1	Design					77:82	Design	77:82	Design	77:82	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	1	8	theme	immune	315:320	arg1	system					322:327	immune system	315:327	immune system	315:327	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	1	8	theme	immune	315:320	arg1	methotrexate					249:260	methotrexate	249:260	methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant	249:339	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	7	9	theme	improved	1103:1110	arg1	parameters					1128:1137	the improved pharmacokinetic parameters	1099:1137	the improved pharmacokinetic parameters of MTX loaded in γCD-MOF	1099:1162	The in vivo experiments showed the improved pharmacokinetic parameters of MTX loaded in γCD-MOF.					
32279736	3	10	theme	MAS	562:564	arg1	NMR					574:576	solid-state 13C MAS CP/TOSS NMR	546:576	solid-state 13C MAS CP/TOSS NMR	546:576	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	6	11	theme	same	983:986	arg1	time					988:991	the same time	979:991	the same time	979:991	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	7	12	theme	pharmacokinetic	1112:1126	arg1	parameters					1128:1137	the improved pharmacokinetic parameters	1099:1137	the improved pharmacokinetic parameters of MTX loaded in γCD-MOF	1099:1162	The in vivo experiments showed the improved pharmacokinetic parameters of MTX loaded in γCD-MOF.					
32279736	3	13	theme	solid-state	546:556	arg1	NMR					574:576	solid-state 13C MAS CP/TOSS NMR	546:576	solid-state 13C MAS CP/TOSS NMR	546:576	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	6	14	theme	complex	901:907	arg1	formation					909:917	Inclusion complex formation	891:917	Inclusion complex formation of MTX with γ-CD	891:934	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	0	15	dep	in	103:104	arg1	vitro					106:110	vitro	106:110	vitro	106:110	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	1	16	theme	metal-organic	153:165	arg1	carriers					236:243	carriers	236:243	carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant	236:339	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	1	16	theme	metal-organic	153:165	arg1	frameworks					167:176	metal-organic frameworks	153:176	metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF)	153:217	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	5	17	theme	MTX	750:752	arg1	dissolution					735:745	The faster dissolution	724:745	The faster dissolution of MTX incorporated in γCD-MOF	724:776	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	6	18	theme	Inclusion	891:899	arg1	formation					909:917	Inclusion complex formation	891:917	Inclusion complex formation of MTX with γ-CD	891:934	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	7	19	theme	MTX	1142:1144	arg1	parameters					1128:1137	the improved pharmacokinetic parameters	1099:1137	the improved pharmacokinetic parameters of MTX loaded in γCD-MOF	1099:1162	The in vivo experiments showed the improved pharmacokinetic parameters of MTX loaded in γCD-MOF.					
32279736	5	20	theme	biorelevant	816:826	arg1	media					828:832	biorelevant media	816:832	biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids	816:888	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	0	21	theme	metal-organic	20:32	arg1	frameworks					34:43	Methotrexate-loaded metal-organic frameworks	0:43	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin	0:74	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	7	22	dep	in	1072:1073	arg1	vivo					1075:1078	vivo	1075:1078	vivo	1075:1078	The in vivo experiments showed the improved pharmacokinetic parameters of MTX loaded in γCD-MOF.					
32279736	3	23	theme	CP/TOSS	566:572	arg1	NMR					574:576	solid-state 13C MAS CP/TOSS NMR	546:576	solid-state 13C MAS CP/TOSS NMR	546:576	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	3	24	theme	FTIR	527:530	arg1	spectroscopy					532:543	FTIR spectroscopy	527:543	FTIR spectroscopy	527:543	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	5	25	theme	gastrointestinal	866:881	arg1	fluids					883:888	the gastrointestinal fluids	862:888	the gastrointestinal fluids	862:888	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	0	26	theme	Methotrexate-loaded	0:18	arg1	frameworks					34:43	Methotrexate-loaded metal-organic frameworks	0:43	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin	0:74	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	2	27	theme	synthesized	346:356	arg1	γCD-MOF					358:364	The synthesized γCD-MOF	342:364	The synthesized γCD-MOF	342:364	The synthesized γCD-MOF was loaded with MTX by impregnation and co-crystallization.					
32279736	0	28	dep	Design	77:82	arg1	frameworks					34:43	Methotrexate-loaded metal-organic frameworks	0:43	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin	0:74	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	4	29	theme	MTX	652:654	arg1	properties					638:647	Pharmaceutically relevant properties	612:647	Pharmaceutically relevant properties of MTX alone and loaded in γCD-MOF	612:682	Pharmaceutically relevant properties of MTX alone and loaded in γCD-MOF were investigated in vitro and in vivo.					
32279736	0	30	theme	in	116:117	arg1	investigation					124:136	in vitro and in vivo investigation	103:136	in vitro and in vivo investigation	103:136	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	0	30	theme	in	116:117	arg1	Design					77:82	Design	77:82	Design	77:82	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	0	31	dep	in	116:117	arg1	vivo					119:122	vivo	119:122	vivo	119:122	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	1	32	dep	agent	305:309	arg1	suppressant					329:339	suppressant	329:339	suppressant	329:339	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	3	33	theme	obtained	430:437	arg1	composites					439:448	The obtained composites	426:448	The obtained composites	426:448	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	3	34	theme	electron	591:598	arg1	microscopy					600:609	scanning electron microscopy	582:609	scanning electron microscopy	582:609	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	1	35	theme	γ-cyclodextrin	194:207	arg1	basis					185:189	the basis	181:189	the basis of γ-cyclodextrin (γCD-MOF)	181:217	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	6	36	theme	dissolution	954:964	arg1	rate					966:969	the drug dissolution rate	945:969	the drug dissolution rate	945:969	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	0	37	theme	γ-cyclodextrin	61:74	arg1	basis					52:56	the basis	48:56	the basis of γ-cyclodextrin	48:74	Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation.					
32279736	5	38	theme	faster	728:733	arg1	dissolution					735:745	The faster dissolution	724:745	The faster dissolution of MTX incorporated in γCD-MOF	724:776	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	6	39	dep	enhances	936:943	arg1	decreases					1003:1011	decreases	1003:1011	slightly decreases the drug permeability through the lipophilic membrane	994:1065	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	3	40	theme	X-ray	482:486	arg1	diffraction					488:498	powder X-ray diffraction	475:498	powder X-ray diffraction	475:498	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	3	41	theme	scanning	582:589	arg1	microscopy					600:609	scanning electron microscopy	582:609	scanning electron microscopy	582:609	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	3	42	theme	13C	558:560	arg1	NMR					574:576	solid-state 13C MAS CP/TOSS NMR	546:576	solid-state 13C MAS CP/TOSS NMR	546:576	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	6	43	theme	drug	1017:1020	arg1	permeability					1022:1033	the drug permeability	1013:1033	the drug permeability	1013:1033	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	7	44	theme	in	1072:1073	arg1	experiments					1080:1090	The in vivo experiments	1068:1090	The in vivo experiments	1068:1090	The in vivo experiments showed the improved pharmacokinetic parameters of MTX loaded in γCD-MOF.					
32279736	3	45	theme	powder	475:480	arg1	diffraction					488:498	powder X-ray diffraction	475:498	powder X-ray diffraction	475:498	The obtained composites were characterized using powder X-ray diffraction, N2 adsorption/desorption, FTIR spectroscopy, solid-state 13C MAS CP/TOSS NMR and scanning electron microscopy.					
32279736	6	46	theme	lipophilic	1047:1056	arg1	membrane					1058:1065	the lipophilic membrane	1043:1065	the lipophilic membrane	1043:1065	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	5	47	theme	blank	798:802	arg1	buffers					804:810	blank buffers	798:810	blank buffers	798:810	The faster dissolution of MTX incorporated in γCD-MOF was demonstrated in blank buffers and biorelevant media (FaSSGF, FaSSIF) simulating the gastrointestinal fluids.					
32279736	1	48	theme	chemotherapy	292:303	arg1	agent					305:309	chemotherapy agent	292:309	chemotherapy agent	292:309	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	1	48	theme	chemotherapy	292:303	arg1	methotrexate					249:260	methotrexate	249:260	methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant	249:339	In this work, metal-organic frameworks on the basis of γ-cyclodextrin (γCD-MOF) were proposed as carriers for methotrexate (MTX) which is widely used as chemotherapy agent and immune system suppressant.					
32279736	6	49	theme	drug	949:952	arg1	rate					966:969	the drug dissolution rate	945:969	the drug dissolution rate	945:969	Inclusion complex formation of MTX with γ-CD enhances the drug dissolution rate and, at the same time, slightly decreases the drug permeability through the lipophilic membrane.					
32279736	4	50	theme	relevant	629:636	arg1	properties					638:647	Pharmaceutically relevant properties	612:647	Pharmaceutically relevant properties of MTX alone and loaded in γCD-MOF	612:682	Pharmaceutically relevant properties of MTX alone and loaded in γCD-MOF were investigated in vitro and in vivo.					
31981225	5	0	theme	oil	781:783	arg1	Basin					785:789	the Campos oil Basin	770:789	the Campos oil Basin	770:789	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	11	1	theme	potential	1423:1431	arg1	use					1445:1447	a potential alternative use	1421:1447	a potential alternative use	1421:1447	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	11	2	theme	potential	1331:1339	arg1	use					1357:1359	potential pharmacological use	1331:1359	potential pharmacological use	1331:1359	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	11	3	theme	pharmacological	1341:1355	arg1	use					1357:1359	potential pharmacological use	1331:1359	potential pharmacological use	1331:1359	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	5	4	theme	Rio	687:689	arg1	state					702:706	Rio de Janeiro state	687:706	Rio de Janeiro state	687:706	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	9	5	theme	anti-inflammatory	1198:1214	arg1	activity					1216:1223	anti-inflammatory activity	1198:1223	anti-inflammatory activity	1198:1223	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	8	6	located	observed	1035:1042	arg1	samples					1051:1057	all samples	1047:1057	all samples tested in in-vivo assays	1047:1082	Anti-inflammatory activity was observed in all samples tested in in-vivo assays, especially in T. coccinea.					
31981225	8	6	located	observed	1035:1042	arg2	activity					1022:1029	Anti-inflammatory activity	1004:1029	Anti-inflammatory activity	1004:1029	Anti-inflammatory activity was observed in all samples tested in in-vivo assays, especially in T. coccinea.					
31981225	8	6	located	observed	1035:1042	arg1	T. coccinea					1099:1109	T. coccinea	1099:1109	T. coccinea	1099:1109	Anti-inflammatory activity was observed in all samples tested in in-vivo assays, especially in T. coccinea.					
31981225	5	7	theme	de	691:692	arg1	state					702:706	Rio de Janeiro state	687:706	Rio de Janeiro state	687:706	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	8	theme	azooxanthellate	633:647	arg1	corals					654:659	these azooxanthellate hard corals	627:659	these azooxanthellate hard corals	627:659	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	4	9	dep	Tubastraea	481:490	arg1	Scleractinia					499:510	Scleractinia	499:510	Scleractinia	499:510	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	4	9	dep	Tubastraea	481:490	arg1	Dendrophylliidae					520:535	Family Dendrophylliidae	513:535	Family Dendrophylliidae	513:535	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	4	9	dep	Tubastraea	481:490	arg1	known					539:543	known	539:543	known as sun corals	539:557	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	5	10	theme	gas	751:753	arg1	platforms					755:763	oil and gas platforms	743:763	platforms	755:763	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	11	theme	Janeiro	694:700	arg1	state					702:706	Rio de Janeiro state	687:706	Rio de Janeiro state	687:706	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	12	theme	hard	649:652	arg1	corals					654:659	these azooxanthellate hard corals	627:659	these azooxanthellate hard corals	627:659	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	13	from	fouling	732:738	arg1	Basin					785:789	the Campos oil Basin	770:789	the Campos oil Basin	770:789	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	14	theme	platforms	755:763	arg1	fouling					732:738	their fouling	726:738	their fouling of oil and gas platforms from the Campos oil Basin	726:789	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	15	theme	corals	654:659	arg1	introduction					611:622	The introduction	607:622	The introduction of these azooxanthellate hard corals into Brazil	607:671	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	11	16	theme	biological	1491:1500	arg1	invasion					1502:1509	this biological invasion	1486:1509	this biological invasion	1486:1509	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	9	17	from	assays	1187:1192	arg1	fraction					1130:1137	The ethyl acetate fraction	1112:1137	The ethyl acetate fraction from this sample	1112:1154	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	9	17	from	assays	1187:1192	arg1	effective					1165:1173	effective	1165:1173	effective	1165:1173	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	3	18	theme	anti-inflammatory	367:383	arg1	ability					385:391	their anti-inflammatory ability	361:391	their anti-inflammatory ability	361:391	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
31981225	0	19	theme	corals	44:49	arg1	potential					18:26	Anti-inflammatory potential	0:26	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)	0:81	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)					
31981225	6	20	theme	invasive	800:807	arg1	species					809:815	The two invasive species	792:815	The two invasive species	792:815	The two invasive species have successfully expanded along the Brazilian coast and threaten endemic species and biodiversity.					
31981225	1	21	dep	use	108:110	arg1	Brazil					88:93	Brazil	88:93	Brazil	88:93	from Brazil: alternative use for management?					
31981225	10	22	theme	cytotoxic	1279:1287	arg1	responses					1289:1297	cytotoxic responses	1279:1297	cytotoxic responses	1279:1297	Depending on the concentration, this fraction showed cytotoxic responses.					
31981225	11	23	dep	CONCLUSIONS	1300:1310	arg1	have					1326:1329	have	1326:1329	have potential pharmacological use	1326:1359	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	7	24	theme	aplysinopsin	968:979	arg1	analogues					981:989	aplysinopsin analogues	968:989	aplysinopsin analogues (alkaloids)	968:1001	The HPLC-MS and GC-MS data suggest the presence of aplysinopsin analogues (alkaloids).					
31981225	7	24	theme	aplysinopsin	968:979	arg1	alkaloids					992:1000	alkaloids	992:1000	alkaloids	992:1000	The HPLC-MS and GC-MS data suggest the presence of aplysinopsin analogues (alkaloids).					
31981225	4	25	theme	Family	513:518	arg1	Scleractinia					499:510	Scleractinia	499:510	Scleractinia	499:510	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	4	25	theme	Family	513:518	arg1	Dendrophylliidae					520:535	Family Dendrophylliidae	513:535	Family Dendrophylliidae	513:535	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	9	26	theme	ethyl	1116:1120	arg1	fraction					1130:1137	The ethyl acetate fraction	1112:1137	The ethyl acetate fraction from this sample	1112:1154	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	9	26	theme	ethyl	1116:1120	arg1	effective					1165:1173	effective	1165:1173	effective	1165:1173	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	0	27	theme	Anti-inflammatory	0:16	arg1	potential					18:26	Anti-inflammatory potential	0:26	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)	0:81	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)					
31981225	2	28	dep	OBJECTIVES	128:137	arg1	was					153:155	was	153:155	was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	153:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	9	29	theme	acetate	1122:1128	arg1	fraction					1130:1137	The ethyl acetate fraction	1112:1137	The ethyl acetate fraction from this sample	1112:1154	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	9	29	theme	acetate	1122:1128	arg1	effective					1165:1173	effective	1165:1173	effective	1165:1173	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	6	30	theme	endemic	883:889	arg1	species					891:897	endemic species	883:897	endemic species	883:897	The two invasive species have successfully expanded along the Brazilian coast and threaten endemic species and biodiversity.					
31981225	0	31	theme	invasive	31:38	arg1	corals					44:49	invasive sun corals	31:49	invasive sun corals	31:49	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)					
31981225	3	32	theme	Methanolic	285:294	arg1	extracts					296:303	Methanolic extracts	285:303	Methanolic extracts	285:303	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
31981225	7	33	theme	analogues	981:989	arg1	presence					956:963	the presence	952:963	the presence of aplysinopsin analogues (alkaloids)	952:1001	The HPLC-MS and GC-MS data suggest the presence of aplysinopsin analogues (alkaloids).					
31981225	2	34	theme	Tubastraea	230:239	arg1	species					222:228	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	34	theme	Tubastraea	230:239	arg1	coccinea					241:248	Tubastraea coccinea	230:248	Tubastraea coccinea	230:248	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	11	35	contain	have	1326:1329	arg1	species					1318:1324	These species	1312:1324	These species	1312:1324	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	11	35	contain	have	1326:1329	arg2	use					1357:1359	potential pharmacological use	1331:1359	potential pharmacological use	1331:1359	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	11	36	theme	invasive	1384:1391	arg1	nature					1393:1398	their invasive nature	1378:1398	their invasive nature	1378:1398	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	11	37	theme	invasion	1502:1509	arg1	management					1472:1481	the management	1468:1481	the management of this biological invasion	1468:1509	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	9	38	from	effective	1165:1173	arg1	assays					1187:1192	in-vitro assays	1178:1192	in-vitro assays for anti-inflammatory activity	1178:1223	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	3	39	theme	chemical	439:446	arg1	analysis					448:455	chemical analysis	439:455	chemical analysis	439:455	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
31981225	4	40	theme	pharmacological	584:598	arg1	value					600:604	pharmacological value	584:604	pharmacological value	584:604	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	4	41	theme	genus	475:479	arg1	Tubastraea					481:490	The genus Tubastraea	471:490	The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals)	471:558	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	0	42	dep	Scleractinia	52:63	arg1	Tubastraea					66:75	Tubastraea	66:75	Tubastraea	66:75	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)					
31981225	9	43	from	sample	1149:1154	arg1	fraction					1130:1137	The ethyl acetate fraction	1112:1137	The ethyl acetate fraction from this sample	1112:1154	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	9	43	from	sample	1149:1154	arg1	effective					1165:1173	effective	1165:1173	effective	1165:1173	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	0	44	theme	sun	40:42	arg1	corals					44:49	invasive sun corals	31:49	invasive sun corals	31:49	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)					
31981225	5	45	theme	oil	743:745	arg1	platforms					755:763	oil and gas platforms	743:763	platforms	755:763	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	2	46	theme	species	222:228	arg1	potential					190:198	the anti-inflammatory potential	168:198	the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	168:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	47	dep	species	222:228	arg1	species					222:228	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	47	dep	species	222:228	arg1	coccinea					241:248	Tubastraea coccinea	230:248	Tubastraea coccinea	230:248	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	47	dep	species	222:228	arg1	tagusensis					265:274	Tubastraea tagusensis	254:274	Tubastraea tagusensis	254:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	4	48	theme	sun	548:550	arg1	corals					552:557	sun corals	548:557	sun corals	548:557	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	5	49	theme	Campos	774:779	arg1	Basin					785:789	the Campos oil Basin	770:789	the Campos oil Basin	770:789	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	2	50	theme	coral	216:220	arg1	species					222:228	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	50	theme	coral	216:220	arg1	coccinea					241:248	Tubastraea coccinea	230:248	Tubastraea coccinea	230:248	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	50	theme	coral	216:220	arg1	tagusensis					265:274	Tubastraea tagusensis	254:274	Tubastraea tagusensis	254:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	1	51	theme	alternative	96:106	arg1	use					108:110	alternative use	96:110	alternative use for management	96:125	from Brazil: alternative use for management?					
31981225	6	52	theme	Brazilian	854:862	arg1	coast					864:868	the Brazilian coast	850:868	the Brazilian coast	850:868	The two invasive species have successfully expanded along the Brazilian coast and threaten endemic species and biodiversity.					
31981225	3	53	theme	synthesized	320:330	arg1	compounds					332:340	synthesized compounds	320:340	synthesized compounds	320:340	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
31981225	4	54	theme	KEY	458:460	arg1	FINDINGS					462:469	KEY FINDINGS	458:469	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.	458:605	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	2	55	theme	invasive	207:214	arg1	species					222:228	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	55	theme	invasive	207:214	arg1	coccinea					241:248	Tubastraea coccinea	230:248	Tubastraea coccinea	230:248	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	55	theme	invasive	207:214	arg1	tagusensis					265:274	Tubastraea tagusensis	254:274	Tubastraea tagusensis	254:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	8	56	theme	in-vivo	1069:1075	arg1	assays					1077:1082	in-vivo assays	1069:1082	in-vivo assays	1069:1082	Anti-inflammatory activity was observed in all samples tested in in-vivo assays, especially in T. coccinea.					
31981225	7	57	theme	GC-MS	933:937	arg1	data					939:942	The HPLC-MS and GC-MS data	917:942	The HPLC-MS and GC-MS data	917:942	The HPLC-MS and GC-MS data suggest the presence of aplysinopsin analogues (alkaloids).					
31981225	11	58	theme	alternative	1433:1443	arg1	use					1445:1447	a potential alternative use	1421:1447	a potential alternative use	1421:1447	CONCLUSIONS These species have potential pharmacological use, and considering their invasive nature, this study presents a potential alternative use, which may enhance the management of this biological invasion.					
31981225	5	59	from	Basin	785:789	arg1	platforms					755:763	oil and gas platforms	743:763	platforms	755:763	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	5	59	from	Basin	785:789	arg1	fouling					732:738	their fouling	726:738	their fouling of oil and gas platforms from the Campos oil Basin	726:789	The introduction of these azooxanthellate hard corals into Brazil, initially in Rio de Janeiro state, occurred through their fouling of oil and gas platforms from the Campos oil Basin.					
31981225	8	60	theme	Anti-inflammatory	1004:1020	arg1	activity					1022:1029	Anti-inflammatory activity	1004:1029	Anti-inflammatory activity	1004:1029	Anti-inflammatory activity was observed in all samples tested in in-vivo assays, especially in T. coccinea.					
31981225	4	61	with	compounds	569:577	arg1	value					600:604	pharmacological value	584:604	pharmacological value	584:604	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	2	62	theme	Tubastraea	254:263	arg1	species					222:228	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	203:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	2	62	theme	Tubastraea	254:263	arg1	tagusensis					265:274	Tubastraea tagusensis	254:274	Tubastraea tagusensis	254:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	7	63	theme	HPLC-MS	921:927	arg1	data					939:942	The HPLC-MS and GC-MS data	917:942	The HPLC-MS and GC-MS data	917:942	The HPLC-MS and GC-MS data suggest the presence of aplysinopsin analogues (alkaloids).					
31981225	4	64	dep	FINDINGS	462:469	arg1	presents					560:567	presents	560:567	presents compounds with pharmacological value	560:604	KEY FINDINGS The genus Tubastraea (Order Scleractinia, Family Dendrophylliidae) (known as sun corals) presents compounds with pharmacological value.					
31981225	2	65	theme	anti-inflammatory	172:188	arg1	potential					190:198	the anti-inflammatory potential	168:198	the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis	168:274	OBJECTIVES The objective was to analyse the anti-inflammatory potential of the invasive coral species Tubastraea coccinea and Tubastraea tagusensis.					
31981225	0	66	dep	potential	18:26	arg1	Scleractinia					52:63	Scleractinia	52:63	Scleractinia	52:63	Anti-inflammatory potential of invasive sun corals (Scleractinia: Tubastraea spp.)					
31981225	9	67	theme	in-vitro	1178:1185	arg1	assays					1187:1192	in-vitro assays	1178:1192	in-vitro assays for anti-inflammatory activity	1178:1223	The ethyl acetate fraction from this sample was more effective in in-vitro assays for anti-inflammatory activity.					
31981225	3	68	dep	METHODS	277:283	arg1	fractions					306:314	fractions	306:314	fractions	306:314	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
31981225	3	68	dep	METHODS	277:283	arg1	compounds					332:340	synthesized compounds	320:340	synthesized compounds	320:340	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
31981225	3	68	dep	METHODS	277:283	arg1	extracts					296:303	Methanolic extracts	285:303	Methanolic extracts	285:303	METHODS Methanolic extracts, fractions and synthesized compounds were evaluated for their anti-inflammatory ability, and their composition was elucidated through chemical analysis.					
33075342	3	0	theme	infrared	439:446	arg1	spectroscopy					448:459	infrared spectroscopy	439:459	infrared spectroscopy	439:459	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	9	1	theme	reinforcing	1549:1559	arg1	agents					1561:1566	reinforcing agents	1549:1566	reinforcing agents	1549:1566	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	3	2	theme	chain	659:663	arg1	groups					665:670	surfactant end chain groups	644:670	surfactant end chain groups	644:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	6	3	theme	lower	1068:1072	arg1	crystallinity					1074:1086	lower crystallinity	1068:1086	lower crystallinity	1068:1086	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	5	4	theme	milling	998:1004	arg1	process					1006:1012	the milling process	994:1012	the milling process	994:1012	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	4	5	theme	electron	736:743	arg1	microscopy					745:754	transmission electron microscopy	723:754	transmission electron microscopy	723:754	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	3	6	theme	cellulosic	518:527	arg1	structure					529:537	a cellulosic structure	516:537	a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups	516:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	5	7	theme	in	908:909	arg1	modified-NC					916:926	the in situ modified-NC	904:926	the in situ modified-NC	904:926	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	8	8	dep	in	1435:1436	arg1	situ					1438:1441	situ	1438:1441	situ	1438:1441	The NC-12S showed smaller particle sizes, high electrostatic, and thermal stability and indicated that 12 h is adequate to prepare modified nanocellulose via in situ modification.					
33075342	5	9	theme	nanocrystal	937:947	arg1	shape					949:953	a nanocrystal shape	935:953	a nanocrystal shape	935:953	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	4	10	theme	transmission	723:734	arg1	microscopy					745:754	transmission electron microscopy	723:754	transmission electron microscopy	723:754	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	8	11	theme	particle	1303:1310	arg1	sizes					1312:1316	smaller particle sizes	1295:1316	smaller particle sizes	1295:1316	The NC-12S showed smaller particle sizes, high electrostatic, and thermal stability and indicated that 12 h is adequate to prepare modified nanocellulose via in situ modification.					
33075342	7	12	theme	superior	1249:1256	arg1	stability					1266:1274	superior thermal stability	1249:1274	superior thermal stability	1249:1274	The modified-NC showed slightly superior thermal stability.					
33075342	6	13	dep	chains	1158:1163	arg1	influence					1179:1187	influence	1179:1187	influence	1179:1187	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	6	13	dep	chains	1158:1163	arg1	addition					1166:1173	addition	1166:1173	addition	1166:1173	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	6	13	dep	chains	1158:1163	arg1	chains					1158:1163	the surfactant chains	1143:1163	the surfactant chains' addition and influence	1143:1187	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	3	14	theme	photoelectron	471:483	arg1	spectroscopy					485:496	X-ray photoelectron spectroscopy	465:496	X-ray photoelectron spectroscopy	465:496	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	5	15	dep	in	908:909	arg1	situ					911:914	situ	911:914	situ	911:914	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	8	16	theme	smaller	1295:1301	arg1	sizes					1312:1316	smaller particle sizes	1295:1316	smaller particle sizes	1295:1316	The NC-12S showed smaller particle sizes, high electrostatic, and thermal stability and indicated that 12 h is adequate to prepare modified nanocellulose via in situ modification.					
33075342	2	17	theme	surface	317:323	arg1	composition					325:335	surface composition	317:335	surface composition	317:335	NCs were characterized for their chemical structure, surface composition, dimension and stability, morphology, crystalline structure, and thermal stability.					
33075342	2	18	theme	crystalline	375:385	arg1	structure					387:395	crystalline structure	375:395	crystalline structure	375:395	NCs were characterized for their chemical structure, surface composition, dimension and stability, morphology, crystalline structure, and thermal stability.					
33075342	6	19	theme	surfactant	1147:1156	arg1	influence					1179:1187	influence	1179:1187	influence	1179:1187	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	6	19	theme	surfactant	1147:1156	arg1	addition					1166:1173	addition	1166:1173	addition	1166:1173	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	6	19	theme	surfactant	1147:1156	arg1	chains					1158:1163	the surfactant chains	1143:1163	the surfactant chains' addition and influence	1143:1187	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	4	20	theme	electrostatic	830:842	arg1	stability					844:852	good electrostatic stability	825:852	good electrostatic stability	825:852	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	7	21	theme	thermal	1258:1264	arg1	stability					1266:1274	superior thermal stability	1249:1274	superior thermal stability	1249:1274	The modified-NC showed slightly superior thermal stability.					
33075342	1	22	theme	present	83:89	arg1	research					91:98	the present research	79:98	the present research	79:98	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	4	23	theme	good	825:828	arg1	stability					844:852	good electrostatic stability	825:852	good electrostatic stability	825:852	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	1	24	used	used	119:122	arg2	straw					109:113	soybean straw	101:113	soybean straw	101:113	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	1	25	theme	ball	160:163	arg1	mill					165:168	a ball mill	158:168	a ball mill	158:168	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	9	26	dep	coatings	1507:1514	arg1	agents					1561:1566	reinforcing agents	1549:1566	reinforcing agents	1549:1566	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	4	27	theme	Zeta	703:706	arg1	potential					708:716	Zeta potential	703:716	Zeta potential	703:716	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	1	28	dep	prepare	127:133	arg1	in-situ					219:225	in-situ	219:225	in-situ	219:225	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	1	28	dep	prepare	127:133	arg1	times					192:196	different milling times	174:196	different milling times (6, 9, and 12 h)	174:213	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	1	28	dep	prepare	127:133	arg1	modified					227:234	modified	227:234	modified with an anionic surfactant	227:261	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	3	29	dep	spectroscopy	448:459	arg1	results					498:504	results	498:504	results	498:504	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	3	30	dep	Fourier	421:427	arg1	transform					429:437	transform	429:437	transform infrared spectroscopy and X-ray photoelectron spectroscopy results	429:504	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	1	31	theme	soybean	101:107	arg1	straw					109:113	soybean straw	101:113	soybean straw	101:113	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	6	32	theme	crystal	1092:1098	arg1	size					1100:1103	crystal size	1092:1103	crystal size	1092:1103	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	2	33	theme	thermal	402:408	arg1	stability					410:418	thermal stability	402:418	thermal stability	402:418	NCs were characterized for their chemical structure, surface composition, dimension and stability, morphology, crystalline structure, and thermal stability.					
33075342	6	34	theme	unmodified	1110:1119	arg1	nanocelluloses					1121:1134	unmodified nanocelluloses	1110:1134	unmodified nanocelluloses	1110:1134	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	3	35	theme	nanocellulose	616:628	arg1	hydroxyls					630:638	nanocellulose hydroxyls	616:638	nanocellulose hydroxyls	616:638	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	4	36	theme	in	775:776	arg1	samples					792:798	the in situ modified samples	771:798	the in situ modified samples	771:798	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	9	37	theme	prepared	1461:1468	arg1	coatings					1507:1514	coatings	1507:1514	coatings	1507:1514	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	9	37	theme	prepared	1461:1468	arg1	emulsifiers					1517:1527	emulsifiers	1517:1527	emulsifiers	1517:1527	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	9	37	theme	prepared	1461:1468	arg1	samples					1470:1476	The prepared samples	1457:1476	The prepared samples	1457:1476	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	9	37	theme	prepared	1461:1468	arg1	nanocomposites					1534:1547	nanocomposites	1534:1547	nanocomposites	1534:1547	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	0	38	theme	modified	33:40	arg1	nanocellulose					42:54	modified nanocellulose	33:54	modified nanocellulose	33:54	Innovative process for obtaining modified nanocellulose from soybean straw.					
33075342	3	39	theme	electronic	585:594	arg1	attractions					596:606	the electronic attractions	581:606	the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups	581:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	3	40	theme	physical	553:560	arg1	interaction					562:572	a physical interaction	551:572	a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups	551:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	1	41	theme	different	174:182	arg1	times					192:196	different milling times	174:196	different milling times (6, 9, and 12 h)	174:213	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	0	42	theme	soybean	61:67	arg1	straw					69:73	soybean straw	61:73	soybean straw	61:73	Innovative process for obtaining modified nanocellulose from soybean straw.					
33075342	8	43	theme	thermal	1343:1349	arg1	stability					1351:1359	thermal stability	1343:1359	thermal stability	1343:1359	The NC-12S showed smaller particle sizes, high electrostatic, and thermal stability and indicated that 12 h is adequate to prepare modified nanocellulose via in situ modification.					
33075342	1	44	dep	times	192:196	arg1	9					202:202	9	202:202	9	202:202	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	1	44	dep	times	192:196	arg1	12 h					209:212	12 h	209:212	12 h	209:212	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	1	44	dep	times	192:196	arg1	6					199:199	6	199:199	6	199:199	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	4	45	theme	smaller	807:813	arg1	sizes					815:819	smaller sizes	807:819	smaller sizes	807:819	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	4	46	theme	light	685:689	arg1	scattering					691:700	The dynamic light scattering	673:700	The dynamic light scattering	673:700	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	8	47	theme	modified	1408:1415	arg1	nanocellulose					1417:1429	modified nanocellulose	1408:1429	modified nanocellulose	1408:1429	The NC-12S showed smaller particle sizes, high electrostatic, and thermal stability and indicated that 12 h is adequate to prepare modified nanocellulose via in situ modification.					
33075342	3	48	theme	surfactant	644:653	arg1	groups					665:670	surfactant end chain groups	644:670	surfactant end chain groups	644:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	5	49	theme	cellulose	1018:1026	arg1	reduction					1034:1042	cellulose scale reduction	1018:1042	cellulose scale reduction	1018:1042	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	4	50	theme	dynamic	677:683	arg1	scattering					691:700	The dynamic light scattering	673:700	The dynamic light scattering	673:700	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	1	51	theme	anionic	244:250	arg1	surfactant					252:261	an anionic surfactant	241:261	an anionic surfactant	241:261	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	3	52	theme	end	655:657	arg1	groups					665:670	surfactant end chain groups	644:670	surfactant end chain groups	644:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	6	53	theme	milling	1200:1206	arg1	process					1208:1214	the milling process	1196:1214	the milling process	1196:1214	The modified-NC showed lower crystallinity and crystal size than unmodified nanocelluloses due to the surfactant chains' addition and influence during the milling process.					
33075342	4	54	theme	modified	783:790	arg1	samples					792:798	the in situ modified samples	771:798	the in situ modified samples	771:798	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	1	55	theme	milling	184:190	arg1	times					192:196	different milling times	174:196	different milling times (6, 9, and 12 h)	174:213	In the present research, soybean straw was used to prepare nanocellulose (NC) via a ball mill, in different milling times (6, 9, and 12 h) and in-situ modified with an anionic surfactant.					
33075342	5	56	theme	scale	1028:1032	arg1	reduction					1034:1042	cellulose scale reduction	1018:1042	cellulose scale reduction	1018:1042	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	8	57	theme	in	1435:1436	arg1	modification					1443:1454	in situ modification	1435:1454	in situ modification	1435:1454	The NC-12S showed smaller particle sizes, high electrostatic, and thermal stability and indicated that 12 h is adequate to prepare modified nanocellulose via in situ modification.					
33075342	5	58	theme	ball	870:873	arg1	mill					875:878	ball mill	870:878	ball mill	870:878	Besides, while ball mill resulted in nanofibers, the in situ modified-NC showed a nanocrystal shape, indicating that the surfactant alters the milling process and cellulose scale reduction.					
33075342	9	59	used	used	1499:1502	arg2	nanocomposites					1534:1547	nanocomposites	1534:1547	nanocomposites	1534:1547	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	9	59	used	used	1499:1502	arg2	emulsifiers					1517:1527	emulsifiers	1517:1527	emulsifiers	1517:1527	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	9	59	used	used	1499:1502	arg2	samples					1470:1476	The prepared samples	1457:1476	The prepared samples	1457:1476	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	9	59	used	used	1499:1502	arg2	coatings					1507:1514	coatings	1507:1514	coatings	1507:1514	The prepared samples could be potentially used as coatings, emulsifiers, and nanocomposites reinforcing agents.					
33075342	2	60	theme	chemical	297:304	arg1	structure					306:314	their chemical structure	291:314	their chemical structure	291:314	NCs were characterized for their chemical structure, surface composition, dimension and stability, morphology, crystalline structure, and thermal stability.					
33075342	4	61	dep	in	775:776	arg1	situ					778:781	situ	778:781	situ	778:781	The dynamic light scattering, Zeta potential, and transmission electron microscopy indicated that the in situ modified samples showed smaller sizes and good electrostatic stability.					
33075342	3	62	theme	due	574:576	arg1	interaction					562:572	a physical interaction	551:572	a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups	551:670	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
33075342	3	63	theme	X-ray	465:469	arg1	spectroscopy					485:496	X-ray photoelectron spectroscopy	465:496	X-ray photoelectron spectroscopy	465:496	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results indicated a cellulosic structure for NCs and a physical interaction due to the electronic attractions between nanocellulose hydroxyls and surfactant end chain groups.					
32931233	7	0	theme	promising	1319:1327	arg1	platform					1345:1352	a promising nanotherapeutic platform	1317:1352	a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis	1317:1469	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	7	0	theme	promising	1319:1327	arg1	CeO2					1309:1312	the core/shell-structured Au@CeO2	1280:1312	the core/shell-structured Au@CeO2	1280:1312	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	4	1	theme	surface	754:760	arg1	light					787:791	the localized surface plasma resonance-induced light	740:791	the localized surface plasma resonance-induced light focusing	740:800	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	7	2	theme	nanotherapeutic	1329:1343	arg1	platform					1345:1352	a promising nanotherapeutic platform	1317:1352	a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis	1317:1469	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	7	2	theme	nanotherapeutic	1329:1343	arg1	CeO2					1309:1312	the core/shell-structured Au@CeO2	1280:1312	the core/shell-structured Au@CeO2	1280:1312	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	2	3	theme	novel	375:379	arg1	improvement					280:290	The radical improvement	268:290	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply	268:368	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	2	3	theme	novel	375:379	arg1	direction					393:401	a novel therapeutic direction	373:401	a novel therapeutic direction for RA	373:408	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	3	4	from	therapy	615:621	arg1	model					641:645	a mouse model	633:645	a mouse model	633:645	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	6	5	theme	RA-induced	1093:1102	arg1	lesions					1104:1110	RA-induced lesions	1093:1110	RA-induced lesions	1093:1110	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	7	6	theme	pathogenesis	1458:1469	arg1	perspective					1443:1453	the perspective	1439:1453	the perspective of pathogenesis	1439:1469	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	4	7	theme	laser	653:657	arg1	irradiation					659:669	laser irradiation	653:669	laser irradiation	653:669	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	5	8	dep	accelerate	993:1002	arg1	relieves					1060:1067	relieves	1060:1067	relieves hypoxia	1060:1075	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	2	9	theme	infiltration	313:324	arg1	direction					393:401	a novel therapeutic direction	373:401	a novel therapeutic direction for RA	373:408	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	2	9	theme	infiltration	313:324	arg1	improvement					280:290	The radical improvement	268:290	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply	268:368	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	2	10	dep	novel	375:379	arg1	therapeutic					381:391	therapeutic	381:391	therapeutic	381:391	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	2	11	theme	oxygen	356:361	arg1	supply					363:368	oxygen supply	356:368	oxygen supply	356:368	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	5	12	theme	elevated	807:814	arg1	temperature					816:826	The elevated temperature	803:826	The elevated temperature	803:826	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	6	13	theme	heat	1254:1257	arg1	cooperation					1239:1249	the cooperation	1235:1249	the cooperation of heat and oxygen	1235:1268	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	5	14	theme	much	1041:1044	arg1	oxygen					1046:1051	much oxygen	1041:1051	much oxygen	1041:1051	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	5	15	theme	catalase-like	958:970	arg1	activity					972:979	the catalase-like activity	954:979	the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia	954:1075	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	2	16	theme	cell	308:311	arg1	infiltration					313:324	inflammatory cell infiltration	295:324	inflammatory cell infiltration	295:324	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	5	17	theme	H2O2	1025:1028	arg1	decomposition					1008:1020	the decomposition	1004:1020	the decomposition of H2O2	1004:1028	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	1	18	theme	progressive	194:204	arg1	dysfunction					212:222	progressive joint dysfunction	194:222	progressive joint dysfunction	194:222	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	18	theme	progressive	194:204	arg1	disability					230:239	disability	230:239	disability	230:239	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	18	theme	progressive	194:204	arg1	death					245:249	death	245:249	death	245:249	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	3	19	theme	near-infrared-absorbing	421:443	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	19	theme	near-infrared-absorbing	421:443	arg1	composite					465:473	a near-infrared-absorbing metal/semiconductor composite	419:473	a near-infrared-absorbing metal/semiconductor composite	419:473	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	2	20	theme	inflammatory	295:306	arg1	infiltration					313:324	inflammatory cell infiltration	295:324	inflammatory cell infiltration	295:324	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	1	21	theme	joint	206:210	arg1	dysfunction					212:222	progressive joint dysfunction	194:222	progressive joint dysfunction	194:222	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	21	theme	joint	206:210	arg1	disability					230:239	disability	230:239	disability	230:239	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	21	theme	joint	206:210	arg1	death					245:249	death	245:249	death	245:249	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	0	22	theme	Plasmon-Boosted	14:28	arg1	Enrichment					42:51	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment	0:51	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.	0:123	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.					
32931233	3	23	theme	topical	566:572	arg1	therapy					615:621	topical photothermal/oxygen-enriched combination therapy	566:621	topical photothermal/oxygen-enriched combination therapy for RA in a mouse model	566:645	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	6	24	theme	factors	1196:1202	arg1	expression					1134:1143	the expression	1130:1143	the expression of proinflammatory cytokines and hypoxia-inducible factors	1130:1202	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	4	25	theme	CeO2	706:709	arg1	conversion					689:698	the photothermal conversion	672:698	the photothermal conversion of Au@CeO2	672:709	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	1	26	theme	Rheumatoid	125:134	arg1	arthritis					136:144	Rheumatoid arthritis	125:144	Rheumatoid arthritis (RA)	125:149	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	26	theme	Rheumatoid	125:134	arg1	disease					168:174	an autoimmune disease	154:174	an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases	154:265	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	26	theme	Rheumatoid	125:134	arg1	RA					147:148	RA	147:148	RA	147:148	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	7	27	theme	core/shell-structured	1284:1304	arg1	platform					1345:1352	a promising nanotherapeutic platform	1317:1352	a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis	1317:1469	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	7	27	theme	core/shell-structured	1284:1304	arg1	CeO2					1309:1312	the core/shell-structured Au@CeO2	1280:1312	the core/shell-structured Au@CeO2	1280:1312	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	0	28	theme	Near-Infrared	0:12	arg1	Enrichment					42:51	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment	0:51	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.	0:123	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.					
32931233	2	29	theme	disorder	344:351	arg1	direction					393:401	a novel therapeutic direction	373:401	a novel therapeutic direction for RA	373:408	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	2	29	theme	disorder	344:351	arg1	improvement					280:290	The radical improvement	268:290	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply	268:368	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	3	30	theme	polyethylene	476:487	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	30	theme	polyethylene	476:487	arg1	CeO2					541:544	Au@CeO2	538:544	Au@CeO2	538:544	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	30	theme	polyethylene	476:487	arg1	composite					465:473	a near-infrared-absorbing metal/semiconductor composite	419:473	a near-infrared-absorbing metal/semiconductor composite	419:473	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	5	31	theme	diseased	913:920	arg1	joints					922:927	diseased joints	913:927	diseased joints	913:927	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	6	32	theme	hypoxia-inducible	1178:1194	arg1	factors					1196:1202	hypoxia-inducible factors	1178:1202	hypoxia-inducible factors	1178:1202	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	2	33	theme	resulting	334:342	arg1	disorder					344:351	the resulting disorder	330:351	the resulting disorder	330:351	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	6	34	theme	proinflammatory	1148:1162	arg1	cytokines					1164:1172	proinflammatory cytokines	1148:1172	proinflammatory cytokines	1148:1172	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	0	35	theme	Heat/Oxygen	30:40	arg1	Enrichment					42:51	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment	0:51	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.	0:123	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.					
32931233	3	36	theme	photothermal/oxygen-enriched	574:601	arg1	therapy					615:621	topical photothermal/oxygen-enriched combination therapy	566:621	topical photothermal/oxygen-enriched combination therapy for RA in a mouse model	566:645	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	4	37	theme	localized	744:752	arg1	light					787:791	the localized surface plasma resonance-induced light	740:791	the localized surface plasma resonance-induced light focusing	740:800	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	3	38	theme	Au	538:539	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	38	theme	Au	538:539	arg1	CeO2					541:544	Au@CeO2	538:544	Au@CeO2	538:544	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	39	theme	metal/semiconductor	445:463	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	39	theme	metal/semiconductor	445:463	arg1	composite					465:473	a near-infrared-absorbing metal/semiconductor composite	419:473	a near-infrared-absorbing metal/semiconductor composite	419:473	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	4	40	theme	@	705:705	arg1	CeO2					706:709	Au@CeO2	703:709	Au@CeO2	703:709	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	2	41	from	improvement	280:290	arg1	supply					363:368	oxygen supply	356:368	oxygen supply	356:368	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	3	42	theme	gold	524:527	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	42	theme	gold	524:527	arg1	CeO2					541:544	Au@CeO2	538:544	Au@CeO2	538:544	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	42	theme	gold	524:527	arg1	composite					465:473	a near-infrared-absorbing metal/semiconductor composite	419:473	a near-infrared-absorbing metal/semiconductor composite	419:473	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	43	theme	glycol-modified	489:503	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	43	theme	glycol-modified	489:503	arg1	CeO2					541:544	Au@CeO2	538:544	Au@CeO2	538:544	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	43	theme	glycol-modified	489:503	arg1	composite					465:473	a near-infrared-absorbing metal/semiconductor composite	419:473	a near-infrared-absorbing metal/semiconductor composite	419:473	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	5	44	theme	hyperproliferative	863:880	arg1	cells					895:899	hyperproliferative inflammatory cells	863:899	hyperproliferative inflammatory cells gathered in diseased joints	863:927	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	4	45	theme	Au	703:704	arg1	CeO2					706:709	Au@CeO2	703:709	Au@CeO2	703:709	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	5	46	theme	inflammatory	882:893	arg1	cells					895:899	hyperproliferative inflammatory cells	863:899	hyperproliferative inflammatory cells gathered in diseased joints	863:927	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	4	47	theme	resonance-induced	769:785	arg1	light					787:791	the localized surface plasma resonance-induced light	740:791	the localized surface plasma resonance-induced light focusing	740:800	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	0	48	theme	Rheumatoid	67:76	arg1	Arthritis					78:86	Rheumatoid Arthritis	67:86	Rheumatoid Arthritis with Metal/Semiconductor Composites	67:122	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.					
32931233	4	49	theme	plasma	762:767	arg1	light					787:791	the localized surface plasma resonance-induced light	740:791	the localized surface plasma resonance-induced light focusing	740:800	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	7	50	theme	light-driven	1376:1387	arg1	enrichment					1401:1410	light-driven heat/oxygen enrichment	1376:1410	light-driven heat/oxygen enrichment	1376:1410	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	3	51	theme	@	540:540	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	51	theme	@	540:540	arg1	CeO2					541:544	Au@CeO2	538:544	Au@CeO2	538:544	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	6	52	theme	oxygen	1263:1268	arg1	cooperation					1239:1249	the cooperation	1235:1249	the cooperation of heat and oxygen	1235:1268	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	3	53	theme	combination	603:613	arg1	therapy					615:621	topical photothermal/oxygen-enriched combination therapy	566:621	topical photothermal/oxygen-enriched combination therapy for RA in a mouse model	566:645	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	6	54	theme	cytokines	1164:1172	arg1	expression					1134:1143	the expression	1130:1143	the expression of proinflammatory cytokines and hypoxia-inducible factors	1130:1202	Significantly, RA-induced lesions are improved, and the expression of proinflammatory cytokines and hypoxia-inducible factors is effectively repressed under the cooperation of heat and oxygen.					
32931233	0	55	with	Arthritis	78:86	arg1	Composites					113:122	Metal/Semiconductor Composites	93:122	Metal/Semiconductor Composites	93:122	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.					
32931233	1	56	theme	autoimmune	157:166	arg1	arthritis					136:144	Rheumatoid arthritis	125:144	Rheumatoid arthritis (RA)	125:149	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	56	theme	autoimmune	157:166	arg1	disease					168:174	an autoimmune disease	154:174	an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases	154:265	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	1	57	theme	severe	254:259	arg1	cases					261:265	severe cases	254:265	severe cases	254:265	Rheumatoid arthritis (RA) is an autoimmune disease that often causes progressive joint dysfunction, even disability and death in severe cases.					
32931233	0	58	theme	Metal/Semiconductor	93:111	arg1	Composites					113:122	Metal/Semiconductor Composites	93:122	Metal/Semiconductor Composites	93:122	Near-Infrared Plasmon-Boosted Heat/Oxygen Enrichment for Reversing Rheumatoid Arthritis with Metal/Semiconductor Composites.					
32931233	2	59	theme	radical	272:278	arg1	direction					393:401	a novel therapeutic direction	373:401	a novel therapeutic direction for RA	373:408	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	2	59	theme	radical	272:278	arg1	improvement					280:290	The radical improvement	268:290	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply	268:368	The radical improvement of inflammatory cell infiltration and the resulting disorder in oxygen supply is a novel therapeutic direction for RA.					
32931233	5	60	theme	ceria	984:988	arg1	activity					972:979	the catalase-like activity	954:979	the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia	954:1075	The elevated temperature can not only remarkably obliterate hyperproliferative inflammatory cells gathered in diseased joints but also vastly increase the catalase-like activity of ceria to accelerate the decomposition of H2O2 to produce much oxygen, which relieves hypoxia.					
32931233	3	61	theme	ceria-shell-coated	505:522	arg1	nanorod					529:535	polyethylene glycol-modified ceria-shell-coated gold nanorod	476:535	polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2)	476:545	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	61	theme	ceria-shell-coated	505:522	arg1	CeO2					541:544	Au@CeO2	538:544	Au@CeO2	538:544	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	3	61	theme	ceria-shell-coated	505:522	arg1	composite					465:473	a near-infrared-absorbing metal/semiconductor composite	419:473	a near-infrared-absorbing metal/semiconductor composite	419:473	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	4	62	theme	photothermal	676:687	arg1	conversion					689:698	the photothermal conversion	672:698	the photothermal conversion of Au@CeO2	672:709	Upon laser irradiation, the photothermal conversion of Au@CeO2 is exponentially enhanced by the localized surface plasma resonance-induced light focusing.					
32931233	7	63	theme	@	1308:1308	arg1	platform					1345:1352	a promising nanotherapeutic platform	1317:1352	a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis	1317:1469	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	7	63	theme	@	1308:1308	arg1	CeO2					1309:1312	the core/shell-structured Au@CeO2	1280:1312	the core/shell-structured Au@CeO2	1280:1312	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	7	64	theme	heat/oxygen	1389:1399	arg1	enrichment					1401:1410	light-driven heat/oxygen enrichment	1376:1410	light-driven heat/oxygen enrichment	1376:1410	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	3	65	theme	mouse	635:639	arg1	model					641:645	a mouse model	633:645	a mouse model	633:645	Herein, a near-infrared-absorbing metal/semiconductor composite, polyethylene glycol-modified ceria-shell-coated gold nanorod (Au@CeO2), is fabricated for topical photothermal/oxygen-enriched combination therapy for RA in a mouse model.					
32931233	7	66	theme	Au	1306:1307	arg1	platform					1345:1352	a promising nanotherapeutic platform	1317:1352	a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis	1317:1469	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32931233	7	66	theme	Au	1306:1307	arg1	CeO2					1309:1312	the core/shell-structured Au@CeO2	1280:1312	the core/shell-structured Au@CeO2	1280:1312	Overall, the core/shell-structured Au@CeO2 is a promising nanotherapeutic platform that can well realize light-driven heat/oxygen enrichment to completely cure RA from the perspective of pathogenesis.					
32707243	1	0	theme	paclitaxel	462:471	arg1	release					428:434	controlled release	417:434	controlled release of temozolomide (TMZ) and paclitaxel (PTX)	417:477	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	1	theme	temozolomide	439:450	arg1	release					428:434	controlled release	417:434	controlled release of temozolomide (TMZ) and paclitaxel (PTX)	417:477	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	8	2	theme	anticancer	1731:1740	arg1	agents					1742:1747	anticancer agents	1731:1747	anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1731:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	5	3	theme	5 wt	1231:1234	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					1237:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	1215:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min	1215:1283	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	7	4	theme	MG	1531:1532	arg1	cells					1534:1538	U-87 MG cells	1526:1538	U-87 MG cells	1526:1538	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	2	5	theme	X-ray	654:658	arg1	diffraction					660:670	X-ray diffraction	654:670	X-ray diffraction (XRD)	654:676	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	2	5	theme	X-ray	654:658	arg1	XRD					673:675	XRD	673:675	XRD	673:675	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	7	6	theme	optimum	1481:1487	arg1	carrier					1501:1507	the optimum nanofibrous carrier	1477:1507	the optimum nanofibrous carrier for apoptosis of U-87 MG cells	1477:1538	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	5	7	theme	loaded-CS-g-PAA-PTX-TMZ/PU	1237:1262	arg1	fibers					1264:1269	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	1215:1269	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min	1215:1283	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	2	8	theme	electron	795:802	arg1	SEM					816:818	SEM	816:818	SEM	816:818	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	2	8	theme	electron	795:802	arg1	microscopy					804:813	scanning electron microscopy	786:813	scanning electron microscopy (SEM)	786:819	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	5	9	theme	MIL-53	1224:1229	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					1237:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	1215:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min	1215:1283	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	1	10	theme	MG	492:493	arg1	cells					508:512	U-87 MG glioblastoma cells	487:512	U-87 MG glioblastoma cells	487:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	11	theme	controlled	417:426	arg1	release					428:434	controlled release	417:434	controlled release of temozolomide (TMZ) and paclitaxel (PTX)	417:477	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	2	12	theme	MIL-53	589:594	arg1	NMOFs					596:600	The synthesized magnetic MIL-53 NMOFs	564:600	The synthesized magnetic MIL-53 NMOFs	564:600	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	0	13	theme	temozolomide	155:166	arg1	co-delivery					140:150	co-delivery	140:150	co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	140:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	8	14	theme	MG	1789:1790	arg1	cells					1805:1809	U-87 MG glioblastoma cells	1784:1809	U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1784:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	0	15	theme	paclitaxel	172:181	arg1	co-delivery					140:150	co-delivery	140:150	co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	140:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	8	16	theme	cells	1805:1809	arg1	cells					1805:1809	U-87 MG glioblastoma cells	1784:1809	U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1784:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	16	theme	cells	1805:1809	arg1	%					1779:1779	49.6%	1775:1779	49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1775:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	3	17	theme	5 wt	885:888	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					891:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	869:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	869:923	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	6	18	theme	non-Fickian	1330:1340	arg1	diffusion					1342:1350	the non-Fickian diffusion	1326:1350	the non-Fickian diffusion of Korsmeyer-Peppas equation	1326:1379	The release data were best described by the non-Fickian diffusion of Korsmeyer-Peppas equation.					
32707243	3	19	theme	magnetic	869:876	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					891:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	869:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	869:923	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	8	20	theme	chemotherapy/hyperthermia	1828:1852	arg1	therapy					1866:1872	chemotherapy/hyperthermia combination therapy	1828:1872	chemotherapy/hyperthermia combination therapy	1828:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	4	21	from	nanofibers	1086:1095	arg1	rate					1052:1055	the release rate	1040:1055	the release rate of TMZ and PTX from magnetic nanofibers	1040:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	4	22	theme	hyperthermia	1024:1035	arg1	effect					1014:1019	The effect	1010:1019	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers	1010:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	0	23	theme	glioblastoma	191:202	arg1	cells					211:215	glioblastoma cancer cells	191:215	glioblastoma cancer cells	191:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	0	24	theme	organic	86:92	arg1	nanofibers					125:134	MIL-53 metal organic framework composite core-shell nanofibers	73:134	MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	73:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	3	25	theme	loaded-CS-g-PAA-PTX-TMZ/PU	891:916	arg1	fibers					918:923	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	869:923	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	869:923	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	2	26	theme	synthesized	568:578	arg1	NMOFs					596:600	The synthesized magnetic MIL-53 NMOFs	564:600	The synthesized magnetic MIL-53 NMOFs	564:600	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	3	27	theme	PTX	843:845	arg1	profiles					855:862	The TMZ and PTX release profiles	831:862	profiles	855:862	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	0	28	theme	composite	104:112	arg1	nanofibers					125:134	MIL-53 metal organic framework composite core-shell nanofibers	73:134	MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	73:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	5	29	theme	magnetic	1130:1137	arg1	field					1139:1143	An alternating magnetic field	1115:1143	An alternating magnetic field	1115:1143	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	3	30	from	fibers	918:923	arg1	TMZ					835:837	The TMZ and PTX release profiles	831:862	TMZ	835:837	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	3	30	from	fibers	918:923	arg1	profiles					855:862	The TMZ and PTX release profiles	831:862	profiles	855:862	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	7	31	theme	cell	1386:1389	arg1	viability					1391:1399	The cell viability	1382:1399	The cell viability	1382:1399	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	4	32	theme	TMZ	1060:1062	arg1	rate					1052:1055	the release rate	1040:1055	the release rate of TMZ and PTX from magnetic nanofibers	1040:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	4	33	theme	PTX	1068:1070	arg1	rate					1052:1055	the release rate	1040:1055	the release rate of TMZ and PTX from magnetic nanofibers	1040:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	8	34	theme	synthesized	1590:1600	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	34	theme	synthesized	1590:1600	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	0	35	theme	poly	15:18	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of poly(acrylic acid)	0:32	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	8	36	theme	MIL-53	1611:1616	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	36	theme	MIL-53	1611:1616	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	2	37	dep	infrared	729:736	arg1	FTIR					739:742	FTIR	739:742	FTIR	739:742	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	0	38	theme	acrylic	20:26	arg1	poly					15:18	poly	15:18	poly(acrylic acid)	15:32	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	0	38	theme	acrylic	20:26	arg1	acid					28:31	acrylic acid	20:31	acrylic acid	20:31	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	1	39	theme	poly	329:332	arg1	nanofibers					402:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers	329:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells	329:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	3	40	theme	43 °C	1003:1007	arg1	temperatures					980:991	temperatures	980:991	temperatures of 37 and 43 °C	980:1007	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	8	41	theme	loaded-	1624:1630	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	41	theme	loaded-	1624:1630	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	1	42	theme	acrylic	334:340	arg1	poly					329:332	poly	329:332	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells	329:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	42	theme	acrylic	334:340	arg1	acid					342:345	acrylic acid	334:345	acrylic acid	334:345	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	3	43	theme	37	996:997	arg1	temperatures					980:991	temperatures	980:991	temperatures of 37 and 43 °C	980:1007	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	1	44	theme	combined	547:554	arg1	method					556:561	chemotherapy/hyperthermia combined method	521:561	chemotherapy/hyperthermia combined method	521:561	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	45	theme	magnetic	244:251	arg1	particles					288:296	the magnetic MIL-53 nanometal organic framework particles	240:296	the magnetic MIL-53 nanometal organic framework particles (NMOFs)	240:304	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	45	theme	magnetic	244:251	arg1	NMOFs					299:303	NMOFs	299:303	NMOFs	299:303	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	8	46	theme	shell	1663:1667	arg1	rate					1674:1677	shell flow rate	1663:1677	shell flow rate: 0.8 mLh-1	1663:1688	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	4	47	from	effect	1014:1019	arg1	rate					1052:1055	the release rate	1040:1055	the release rate of TMZ and PTX from magnetic nanofibers	1040:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	7	48	theme	cells	1534:1538	arg1	apoptosis					1513:1521	apoptosis	1513:1521	apoptosis of U-87 MG cells	1513:1538	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	4	49	theme	magnetic	1077:1084	arg1	nanofibers					1086:1095	magnetic nanofibers	1077:1095	magnetic nanofibers	1077:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	8	50	theme	targeted	1710:1717	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	50	theme	targeted	1710:1717	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	1	51	theme	framework	278:286	arg1	particles					288:296	the magnetic MIL-53 nanometal organic framework particles	240:296	the magnetic MIL-53 nanometal organic framework particles (NMOFs)	240:304	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	51	theme	framework	278:286	arg1	NMOFs					299:303	NMOFs	299:303	NMOFs	299:303	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	5	52	theme	mild	1162:1165	arg1	43 °C					1181:1185	43 °C	1181:1185	43 °C	1181:1185	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	5	52	theme	mild	1162:1165	arg1	hyperthermia					1167:1178	the mild hyperthermia	1158:1178	the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min	1158:1283	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	7	53	theme	U-87	1526:1529	arg1	cells					1534:1538	U-87 MG cells	1526:1538	U-87 MG cells	1526:1538	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	5	54	theme	%	1235:1235	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					1237:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	1215:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min	1215:1283	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	7	55	theme	nanofibrous	1489:1499	arg1	carrier					1501:1507	the optimum nanofibrous carrier	1477:1507	the optimum nanofibrous carrier for apoptosis of U-87 MG cells	1477:1538	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	8	56	theme	agents	1742:1747	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	56	theme	agents	1742:1747	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	7	57	dep	Bcl-2	1420:1424	arg1	levels					1442:1447	expression levels	1431:1447	expression levels	1431:1447	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	2	58	theme	scanning	786:793	arg1	SEM					816:818	SEM	816:818	SEM	816:818	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	2	58	theme	scanning	786:793	arg1	microscopy					804:813	scanning electron microscopy	786:813	scanning electron microscopy (SEM)	786:819	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	6	59	theme	release	1290:1296	arg1	data					1298:1301	The release data	1286:1301	The release data	1286:1301	The release data were best described by the non-Fickian diffusion of Korsmeyer-Peppas equation.					
32707243	1	60	theme	U-87	487:490	arg1	cells					508:512	U-87 MG glioblastoma cells	487:512	U-87 MG glioblastoma cells	487:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	8	61	theme	maximum	1754:1760	arg1	apoptosis					1762:1770	maximum apoptosis	1754:1770	maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1754:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	1	62	theme	glioblastoma	495:506	arg1	cells					508:512	U-87 MG glioblastoma cells	487:512	U-87 MG glioblastoma cells	487:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	63	theme	present	225:231	arg1	study					233:237	the present study	221:237	the present study	221:237	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	8	64	dep	nanofibers	1651:1660	arg1	rate					1674:1677	shell flow rate	1663:1677	shell flow rate: 0.8 mLh-1	1663:1688	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	65	theme	%	1779:1779	arg1	apoptosis					1762:1770	maximum apoptosis	1754:1770	maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1754:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	2	66	theme	magnetic	580:587	arg1	NMOFs					596:600	The synthesized magnetic MIL-53 NMOFs	564:600	The synthesized magnetic MIL-53 NMOFs	564:600	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	7	67	theme	expression	1431:1440	arg1	levels					1442:1447	expression levels	1431:1447	expression levels	1431:1447	The cell viability, flowcytometry and Bcl-2, Bax expression levels were investigated to obtain the optimum nanofibrous carrier for apoptosis of U-87 MG cells in vitro.					
32707243	2	68	theme	vibrating-sample	746:761	arg1	VSM					777:779	VSM	777:779	VSM	777:779	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	2	68	theme	vibrating-sample	746:761	arg1	magnetometer					763:774	vibrating-sample magnetometer	746:774	vibrating-sample magnetometer (VSM)	746:780	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	6	69	theme	Korsmeyer-Peppas	1355:1370	arg1	equation					1372:1379	Korsmeyer-Peppas equation	1355:1379	Korsmeyer-Peppas equation	1355:1379	The release data were best described by the non-Fickian diffusion of Korsmeyer-Peppas equation.					
32707243	5	70	theme	alternating	1118:1128	arg1	field					1139:1143	An alternating magnetic field	1115:1143	An alternating magnetic field	1115:1143	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	8	71	theme	U-87	1784:1787	arg1	cells					1805:1809	U-87 MG glioblastoma cells	1784:1809	U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1784:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	2	72	dep	diffraction	660:670	arg1	analysis					821:828	analysis	821:828	analysis	821:828	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	0	73	theme	cancer	204:209	arg1	cells					211:215	glioblastoma cancer cells	191:215	glioblastoma cancer cells	191:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	8	74	theme	glioblastoma	1792:1803	arg1	cells					1805:1809	U-87 MG glioblastoma cells	1784:1809	U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1784:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	3	75	theme	%	889:889	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					891:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	869:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	869:923	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	2	76	theme	NMOF-loaded	606:616	arg1	nanofibers					618:627	NMOF-loaded nanofibers	606:627	NMOF-loaded nanofibers	606:627	The synthesized magnetic MIL-53 NMOFs and NMOF-loaded nanofibers were characterized using X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Fourier transformed infrared (FTIR), vibrating-sample magnetometer (VSM) and scanning electron microscopy (SEM) analysis.					
32707243	8	77	used	used	1700:1703	arg2	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	77	used	used	1700:1703	arg2	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	0	78	theme	metal	80:84	arg1	nanofibers					125:134	MIL-53 metal organic framework composite core-shell nanofibers	73:134	MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	73:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	3	79	theme	MIL-53	878:883	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					891:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	869:916	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers	869:923	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	0	80	theme	framework	94:102	arg1	nanofibers					125:134	MIL-53 metal organic framework composite core-shell nanofibers	73:134	MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	73:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	1	81	theme	grafted-chitosan/polyurethane	348:376	arg1	nanofibers					402:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers	329:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells	329:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	8	82	theme	obtained	1554:1561	arg1	results					1563:1569	The obtained results	1550:1569	The obtained results	1550:1569	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	0	83	theme	core-shell	114:123	arg1	nanofibers					125:134	MIL-53 metal organic framework composite core-shell nanofibers	73:134	MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells	73:215	Fabrication of poly(acrylic acid) grafted-chitosan/polyurethane/magnetic MIL-53 metal organic framework composite core-shell nanofibers for co-delivery of temozolomide and paclitaxel against glioblastoma cancer cells.					
32707243	1	84	theme	PA-g-CS/PU	379:388	arg1	nanofibers					402:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers	329:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells	329:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	8	85	theme	combination	1854:1864	arg1	therapy					1866:1872	chemotherapy/hyperthermia combination therapy	1828:1872	chemotherapy/hyperthermia combination therapy	1828:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	86	with	agents	1742:1747	arg1	apoptosis					1762:1770	maximum apoptosis	1754:1770	maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1754:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	3	87	theme	release	847:853	arg1	profiles					855:862	The TMZ and PTX release profiles	831:862	profiles	855:862	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	1	88	theme	core-shell	391:400	arg1	nanofibers					402:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers	329:411	poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells	329:512	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	3	89	theme	physiological	960:972	arg1	pH					974:975	physiological pH	960:975	physiological pH	960:975	The TMZ and PTX release profiles from magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers were investigated under acidic and physiological pH at temperatures of 37 and 43 °C.					
32707243	8	90	dep	rate	1674:1677	arg1	mLh-1					1684:1688	0.8 mLh-1	1680:1688	shell flow rate: 0.8 mLh-1	1663:1688	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	91	theme	magnetic	1602:1609	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	91	theme	magnetic	1602:1609	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	92	theme	NMOFs	1618:1622	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	92	theme	NMOFs	1618:1622	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	5	93	theme	magnetic	1215:1222	arg1	loaded-CS-g-PAA-PTX-TMZ/PU					1237:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU	1215:1262	magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min	1215:1283	An alternating magnetic field could induce the mild hyperthermia (43 °C) for the cells treated with magnetic MIL-53 5 wt% loaded-CS-g-PAA-PTX-TMZ/PU fibers during 10 min.					
32707243	8	94	theme	PA-g-CS/PU/TMZ-PTX	1632:1649	arg1	delivery					1719:1726	a targeted delivery	1708:1726	a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy	1708:1872	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	8	94	theme	PA-g-CS/PU/TMZ-PTX	1632:1649	arg1	nanofibers					1651:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers	1586:1660	the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1)	1586:1689	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	1	95	theme	nanometal	260:268	arg1	particles					288:296	the magnetic MIL-53 nanometal organic framework particles	240:296	the magnetic MIL-53 nanometal organic framework particles (NMOFs)	240:304	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	95	theme	nanometal	260:268	arg1	NMOFs					299:303	NMOFs	299:303	NMOFs	299:303	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	4	96	theme	release	1044:1050	arg1	rate					1052:1055	the release rate	1040:1055	the release rate of TMZ and PTX from magnetic nanofibers	1040:1095	The effect of hyperthermia on the release rate of TMZ and PTX from magnetic nanofibers was investigated.					
32707243	1	97	theme	chemotherapy/hyperthermia	521:545	arg1	method					556:561	chemotherapy/hyperthermia combined method	521:561	chemotherapy/hyperthermia combined method	521:561	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	6	98	theme	equation	1372:1379	arg1	diffusion					1342:1350	the non-Fickian diffusion	1326:1350	the non-Fickian diffusion of Korsmeyer-Peppas equation	1326:1379	The release data were best described by the non-Fickian diffusion of Korsmeyer-Peppas equation.					
32707243	8	99	theme	flow	1669:1672	arg1	rate					1674:1677	shell flow rate	1663:1677	shell flow rate: 0.8 mLh-1	1663:1688	The obtained results indicated that the synthesized magnetic MIL-53 NMOFs loaded- PA-g-CS/PU/TMZ-PTX nanofibers (shell flow rate: 0.8 mLh-1) could be used as a targeted delivery of anticancer agents with maximum apoptosis of 49.6% of U-87 MG glioblastoma cells under AMF during chemotherapy/hyperthermia combination therapy.					
32707243	1	100	theme	MIL-53	253:258	arg1	particles					288:296	the magnetic MIL-53 nanometal organic framework particles	240:296	the magnetic MIL-53 nanometal organic framework particles (NMOFs)	240:304	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	100	theme	MIL-53	253:258	arg1	NMOFs					299:303	NMOFs	299:303	NMOFs	299:303	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	101	theme	organic	270:276	arg1	particles					288:296	the magnetic MIL-53 nanometal organic framework particles	240:296	the magnetic MIL-53 nanometal organic framework particles (NMOFs)	240:304	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32707243	1	101	theme	organic	270:276	arg1	NMOFs					299:303	NMOFs	299:303	NMOFs	299:303	In the present study, the magnetic MIL-53 nanometal organic framework particles (NMOFs) were incorporated into poly(acrylic acid) grafted-chitosan/polyurethane (PA-g-CS/PU) core-shell nanofibers for controlled release of temozolomide (TMZ) and paclitaxel (PTX) against U-87 MG glioblastoma cells during chemotherapy/hyperthermia combined method.					
32048330	5	0	theme	efficacy	532:539	arg1	profiles					552:559	the efficacy and safety profiles	528:559	the efficacy and safety profiles of UFH	528:566	AIM OF THE STUDY To assess the efficacy and safety profiles of UFH compared with bivalirudin during ECMO support.					
32048330	10	1	theme	time	1179:1182	arg1	aPTT					1223:1226	aPTT	1223:1226	aPTT	1223:1226	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	1	theme	time	1179:1182	arg1	time					1217:1220	time activated partial thromboplastin time	1179:1220	time activated partial thromboplastin time (aPTT) was within the therapeutic range	1179:1260	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	1	2	theme	hematological	188:200	arg1	complications					202:214	hematological complications	188:214	hematological complications	188:214	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	8	3	theme	thrombotic	836:845	arg1	episodes					872:879	composite thrombotic, bleeding, and mortality episodes	826:879	composite thrombotic, bleeding, and mortality episodes	826:879	We analyzed their 7-day rate of composite thrombotic, bleeding, and mortality episodes while on anticoagulation.					
32048330	9	4	dep	%	1139:1139	arg1	P = .58					1151:1157	P = .58	1151:1157	P = .58	1151:1157	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	11	5	theme	mortality	1442:1450	arg1	events					1452:1457	mortality events	1442:1457	mortality events	1442:1457	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	5	6	theme	ECMO	601:604	arg1	support					606:612	ECMO support	601:612	ECMO support	601:612	AIM OF THE STUDY To assess the efficacy and safety profiles of UFH compared with bivalirudin during ECMO support.					
32048330	9	7	dep	%	1043:1043	arg1	P = .24					1054:1060	P = .24	1054:1060	P = .24	1054:1060	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	6	8	dep	METHODS	615:621	arg1	retrospectively					626:640	retrospectively	626:640	retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018	626:734	METHODS We retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018.					
32048330	1	9	theme	BACKGROUND	80:89	arg1	ECMO					128:131	ECMO	128:131	ECMO	128:131	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	1	9	theme	BACKGROUND	80:89	arg1	oxygenation					115:125	BACKGROUND Extracorporeal membrane oxygenation	80:125	BACKGROUND Extracorporeal membrane oxygenation (ECMO)	80:132	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	8	10	theme	bleeding	848:855	arg1	episodes					872:879	composite thrombotic, bleeding, and mortality episodes	826:879	composite thrombotic, bleeding, and mortality episodes	826:879	We analyzed their 7-day rate of composite thrombotic, bleeding, and mortality episodes while on anticoagulation.					
32048330	2	11	from	antithrombotic	262:275	arg1	ECMO					280:283	ECMO	280:283	ECMO	280:283	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	9	12	theme	statistical	929:939	arg1	differences					941:951	no statistical differences	926:951	no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	926:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	13	dep	%	1000:1000	arg1	P = 0.60					1012:1019	P = 0.60	1012:1019	P = 0.60	1012:1019	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	1	14	theme	Extracorporeal	91:104	arg1	ECMO					128:131	ECMO	128:131	ECMO	128:131	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	1	14	theme	Extracorporeal	91:104	arg1	oxygenation					115:125	BACKGROUND Extracorporeal membrane oxygenation	80:125	BACKGROUND Extracorporeal membrane oxygenation (ECMO)	80:132	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	6	15	theme	January	703:709	arg1	2013					711:714	1 January 2013	701:714	1 January 2013	701:714	METHODS We retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018.					
32048330	5	16	theme	UFH	564:566	arg1	profiles					552:559	the efficacy and safety profiles	528:559	the efficacy and safety profiles of UFH	528:566	AIM OF THE STUDY To assess the efficacy and safety profiles of UFH compared with bivalirudin during ECMO support.					
32048330	1	17	theme	membrane	106:113	arg1	ECMO					128:131	ECMO	128:131	ECMO	128:131	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	1	17	theme	membrane	106:113	arg1	oxygenation					115:125	BACKGROUND Extracorporeal membrane oxygenation	80:125	BACKGROUND Extracorporeal membrane oxygenation (ECMO)	80:132	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	9	18	theme	bleeding	1029:1036	arg1	rate					966:969	the 7-day rate	956:969	the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	956:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	12	19	from	range	1584:1588	arg1	comparison					1593:1602	comparison	1593:1602	comparison to UFH	1593:1609	However, it was observed that bivalirudin consistently maintained aPTT within the therapeutic range in comparison to UFH.					
32048330	3	20	theme	heparin-induced	381:395	arg1	thrombocytopenia					397:412	heparin-induced thrombocytopenia	381:412	heparin-induced thrombocytopenia	381:412	However, it bears the risk for heparin-induced thrombocytopenia.					
32048330	10	21	theme	partial	1194:1200	arg1	aPTT					1223:1226	aPTT	1223:1226	aPTT	1223:1226	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	21	theme	partial	1194:1200	arg1	time					1217:1220	time activated partial thromboplastin time	1179:1220	time activated partial thromboplastin time (aPTT) was within the therapeutic range	1179:1260	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	11	22	dep	CONCLUSIONS	1316:1326	arg1	suggests					1339:1346	suggests	1339:1346	suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO	1339:1487	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	5	23	theme	STUDY	512:516	arg1	AIM					501:503	AIM	501:503	AIM OF THE STUDY To assess the efficacy and safety profiles of UFH	501:566	AIM OF THE STUDY To assess the efficacy and safety profiles of UFH compared with bivalirudin during ECMO support.					
32048330	10	24	theme	therapeutic	1244:1254	arg1	range					1256:1260	the therapeutic range	1240:1260	the therapeutic range	1240:1260	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	9	25	theme	mortality	1124:1132	arg1	rate					966:969	the 7-day rate	956:969	the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	956:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	8	26	theme	episodes	872:879	arg1	rate					818:821	their 7-day rate	806:821	their 7-day rate of composite thrombotic, bleeding, and mortality episodes	806:879	We analyzed their 7-day rate of composite thrombotic, bleeding, and mortality episodes while on anticoagulation.					
32048330	9	27	from	differences	941:951	arg1	rate					966:969	the 7-day rate	956:969	the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	956:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	28	theme	7-day	960:964	arg1	rate					966:969	the 7-day rate	956:969	the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	956:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	29	dep	RESULTS	907:913	arg1	were					921:924	were	921:924	were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	921:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	2	30	from	ECMO	280:283	arg1	antithrombotic					262:275	antithrombotic	262:275	antithrombotic	262:275	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	10	31	theme	time	1217:1220	arg1	percentage					1165:1174	The percentage	1161:1174	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range	1161:1260	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	31	theme	time	1217:1220	arg1	higher					1266:1271	higher	1266:1271	higher	1266:1271	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	6	32	from	2013	711:714	arg1	ECMO					691:694	ECMO	691:694	ECMO from 1 January 2013 to 1 September 2018	691:734	METHODS We retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018.					
32048330	11	33	from	rates	1401:1405	arg1	patients					1462:1469	patients	1462:1469	patients supported by ECMO	1462:1487	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	0	34	theme	extracorporeal	43:56	arg1	oxygenation					67:77	extracorporeal membrane oxygenation	43:77	extracorporeal membrane oxygenation	43:77	Heparin vs bivalirudin anticoagulation for extracorporeal membrane oxygenation.					
32048330	8	35	theme	7-day	812:816	arg1	rate					818:821	their 7-day rate	806:821	their 7-day rate of composite thrombotic, bleeding, and mortality episodes	806:879	We analyzed their 7-day rate of composite thrombotic, bleeding, and mortality episodes while on anticoagulation.					
32048330	2	36	theme	Unfractionated	217:230	arg1	UFH					241:243	UFH	241:243	UFH	241:243	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	2	36	theme	Unfractionated	217:230	arg1	heparin					232:238	Unfractionated heparin	217:238	Unfractionated heparin (UFH)	217:244	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	2	36	theme	Unfractionated	217:230	arg1	mainstay					253:260	the mainstay	249:260	the mainstay antithrombotic in ECMO	249:283	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	9	37	theme	composite	974:982	arg1	thrombosis					984:993	composite thrombosis	974:993	composite thrombosis (33.3% vs 26.3%; P = 0.60)	974:1020	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	37	theme	composite	974:982	arg1	%					1000:1000	33.3%	996:1000	33.3%	996:1000	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	37	theme	composite	974:982	arg1	%					1009:1009	26.3%	1005:1009	26.3%	1005:1009	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	10	38	dep	higher	1266:1271	arg1	%					1293:1293	50%	1291:1293	50%	1291:1293	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	38	dep	higher	1266:1271	arg1	%					1302:1302	85.7%	1298:1302	85.7%	1298:1302	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	2	39	theme	antithrombotic	262:275	arg1	heparin					232:238	Unfractionated heparin	217:238	Unfractionated heparin (UFH)	217:244	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	2	39	theme	antithrombotic	262:275	arg1	mainstay					253:260	the mainstay	249:260	the mainstay antithrombotic in ECMO	249:283	Unfractionated heparin (UFH) is the mainstay antithrombotic in ECMO and depends on antithrombin III (AT III) to exhibit its actions.					
32048330	10	40	theme	activated	1184:1192	arg1	aPTT					1223:1226	aPTT	1223:1226	aPTT	1223:1226	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	40	theme	activated	1184:1192	arg1	time					1217:1220	time activated partial thromboplastin time	1179:1220	time activated partial thromboplastin time (aPTT) was within the therapeutic range	1179:1260	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	11	41	theme	thrombosis	1410:1419	arg1	rates					1401:1405	similar rates	1393:1405	similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO	1393:1487	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	9	42	theme	thrombosis	984:993	arg1	rate					966:969	the 7-day rate	956:969	the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58)	956:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	43	theme	in-hospital	1112:1122	arg1	%					1148:1148	36.8%	1144:1148	36.8%	1144:1148	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	43	theme	in-hospital	1112:1122	arg1	mortality					1124:1132	in-hospital mortality	1112:1132	in-hospital mortality (45.5% vs 36.8%; P = .58)	1112:1158	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	43	theme	in-hospital	1112:1122	arg1	%					1139:1139	45.5%	1135:1139	45.5%	1135:1139	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	5	44	theme	safety	545:550	arg1	profiles					552:559	the efficacy and safety profiles	528:559	the efficacy and safety profiles of UFH	528:566	AIM OF THE STUDY To assess the efficacy and safety profiles of UFH compared with bivalirudin during ECMO support.					
32048330	12	45	theme	therapeutic	1572:1582	arg1	range					1584:1588	the therapeutic range	1568:1588	the therapeutic range in comparison to UFH	1568:1609	However, it was observed that bivalirudin consistently maintained aPTT within the therapeutic range in comparison to UFH.					
32048330	0	46	theme	membrane	58:65	arg1	oxygenation					67:77	extracorporeal membrane oxygenation	43:77	extracorporeal membrane oxygenation	43:77	Heparin vs bivalirudin anticoagulation for extracorporeal membrane oxygenation.					
32048330	4	47	theme	thrombin	439:446	arg1	inhibitor					448:456	a direct thrombin inhibitor	430:456	a direct thrombin inhibitor	430:456	Bivalirudin is a direct thrombin inhibitor and is inherently not dependent on AT III.					
32048330	4	47	theme	thrombin	439:446	arg1	Bivalirudin					415:425	Bivalirudin	415:425	Bivalirudin	415:425	Bivalirudin is a direct thrombin inhibitor and is inherently not dependent on AT III.					
32048330	11	48	theme	similar	1393:1399	arg1	rates					1401:1405	similar rates	1393:1405	similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO	1393:1487	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	1	49	theme	hemostatic	142:151	arg1	alterations					153:163	hemostatic alterations	142:163	hemostatic alterations that may contribute to hematological complications	142:214	BACKGROUND Extracorporeal membrane oxygenation (ECMO) induces hemostatic alterations that may contribute to hematological complications.					
32048330	9	50	dep	%	1087:1087	arg1	P = .37					1099:1105	P = .37	1099:1105	P = .37	1099:1105	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	4	51	theme	direct	432:437	arg1	inhibitor					448:456	a direct thrombin inhibitor	430:456	a direct thrombin inhibitor	430:456	Bivalirudin is a direct thrombin inhibitor and is inherently not dependent on AT III.					
32048330	4	51	theme	direct	432:437	arg1	Bivalirudin					415:425	Bivalirudin	415:425	Bivalirudin	415:425	Bivalirudin is a direct thrombin inhibitor and is inherently not dependent on AT III.					
32048330	3	52	contain	bears	362:366	arg1	it					359:360	it	359:360	it	359:360	However, it bears the risk for heparin-induced thrombocytopenia.					
32048330	3	52	contain	bears	362:366	arg2	risk					372:375	the risk	368:375	the risk for heparin-induced thrombocytopenia	368:412	However, it bears the risk for heparin-induced thrombocytopenia.					
32048330	6	53	dep	retrospectively	626:640	arg1	reviewed					642:649	reviewed	642:649	retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018	626:734	METHODS We retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018.					
32048330	11	54	theme	bleeding	1428:1435	arg1	rates					1401:1405	similar rates	1393:1405	similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO	1393:1487	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	8	55	theme	mortality	862:870	arg1	episodes					872:879	composite thrombotic, bleeding, and mortality episodes	826:879	composite thrombotic, bleeding, and mortality episodes	826:879	We analyzed their 7-day rate of composite thrombotic, bleeding, and mortality episodes while on anticoagulation.					
32048330	6	56	theme	adult	654:658	arg1	patients					660:667	52 adult patients	651:667	52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018	651:734	METHODS We retrospectively reviewed 52 adult patients who were supported by ECMO from 1 January 2013 to 1 September 2018.					
32048330	9	57	theme	30-day	1064:1069	arg1	bleeding					1029:1036	bleeding	1029:1036	bleeding	1029:1036	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	9	57	theme	30-day	1064:1069	arg1	mortality					1071:1079	30-day mortality	1064:1079	30-day mortality	1064:1079	RESULTS There were no statistical differences in the 7-day rate of composite thrombosis (33.3% vs 26.3%; P = 0.60), major bleeding (18.2% vs 5.3%; P = .24), 30-day mortality, (42.4% vs 26.3%; P = .37), or in-hospital mortality (45.5% vs 36.8%; P = .58).					
32048330	11	58	theme	events	1452:1457	arg1	rates					1401:1405	similar rates	1393:1405	similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO	1393:1487	CONCLUSIONS This study suggests that UFH and bivalirudin are associated with similar rates of thrombosis, major bleeding, and mortality events in patients supported by ECMO.					
32048330	10	59	theme	thromboplastin	1202:1215	arg1	aPTT					1223:1226	aPTT	1223:1226	aPTT	1223:1226	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	59	theme	thromboplastin	1202:1215	arg1	time					1217:1220	time activated partial thromboplastin time	1179:1220	time activated partial thromboplastin time (aPTT) was within the therapeutic range	1179:1260	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
32048330	10	60	dep	%	1293:1293	arg1	P = .007					1305:1312	P = .007	1305:1312	P = .007	1305:1312	The percentage of time activated partial thromboplastin time (aPTT) was within the therapeutic range was higher with bivalirudin (50% vs 85.7%; P = .007).					
34209115	2	0	theme	Young	417:421	arg1	modulus					425:431	Young's modulus	417:431	Young's modulus	417:431	The mechanical performances (Young's modulus and compressive strength) increased with the filler addition.					
34209115	1	1	theme	new	300:302	arg1	formulations					314:325	new composite formulations	300:325	new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole	300:385	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	0	2	theme	Drug	120:123	arg1	Systems					134:140	Drug Delivery Systems	120:140	Drug Delivery Systems	120:140	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	1	3	dep	polysaccharides	147:161	arg1	polysaccharides					147:161	Two polysaccharides	143:161	Two polysaccharides (cellulose and chitosan)	143:186	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	1	3	dep	polysaccharides	147:161	arg1	chitosan					178:185	chitosan	178:185	chitosan	178:185	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	1	3	dep	polysaccharides	147:161	arg1	cellulose					164:172	cellulose	164:172	cellulose	164:172	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	1	4	theme	composite	304:312	arg1	formulations					314:325	new composite formulations	300:325	new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole	300:385	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	1	5	theme	formulations	314:325	arg1	obtainment					286:295	the obtainment	282:295	the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole	282:385	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	3	6	theme	mucoadhesion	585:596	arg1	parameters					598:607	mucoadhesion parameters	585:607	mucoadhesion parameters	585:607	The nature of the filler used in the studied formulations influenced both bioadhesion and mucoadhesion parameters.					
34209115	5	7	theme	ATCC	932:935	arg1	25922					937:941	Escherichia coli ATCC 25922	915:941	Escherichia coli ATCC 25922	915:941	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	5	8	theme	Escherichia	915:925	arg1	25922					937:941	Escherichia coli ATCC 25922	915:941	Escherichia coli ATCC 25922	915:941	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	0	9	theme	Delivery	125:132	arg1	Systems					134:140	Drug Delivery Systems	120:140	Drug Delivery Systems	120:140	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	4	10	theme	in	717:718	arg1	rate					726:729	the in vitro rate	713:729	the in vitro rate	713:729	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	5	11	theme	aureus	877:882	arg1	25923					889:893	Staphylococcus aureus ATCC 25923	862:893	Staphylococcus aureus ATCC 25923	862:893	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	2	12	theme	compressive	437:447	arg1	strength					449:456	compressive strength	437:456	compressive strength	437:456	The mechanical performances (Young's modulus and compressive strength) increased with the filler addition.					
34209115	5	13	theme	ATCC	884:887	arg1	25923					889:893	Staphylococcus aureus ATCC 25923	862:893	Staphylococcus aureus ATCC 25923	862:893	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	5	14	dep	positive	852:859	arg1	25923					889:893	Staphylococcus aureus ATCC 25923	862:893	Staphylococcus aureus ATCC 25923	862:893	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	4	15	theme	Korsmeyer-Peppas	782:797	arg1	model					799:803	the Korsmeyer-Peppas model	778:803	the Korsmeyer-Peppas model	778:803	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	2	16	dep	performances	403:414	arg1	strength					449:456	compressive strength	437:456	compressive strength	437:456	The mechanical performances (Young's modulus and compressive strength) increased with the filler addition.					
34209115	2	16	dep	performances	403:414	arg1	modulus					425:431	Young's modulus	417:431	Young's modulus	417:431	The mechanical performances (Young's modulus and compressive strength) increased with the filler addition.					
34209115	2	17	theme	filler	478:483	arg1	addition					485:492	the filler addition	474:492	the filler addition	474:492	The mechanical performances (Young's modulus and compressive strength) increased with the filler addition.					
34209115	3	18	theme	filler	513:518	arg1	nature					499:504	The nature	495:504	The nature of the filler used in the studied formulations	495:551	The nature of the filler used in the studied formulations influenced both bioadhesion and mucoadhesion parameters.					
34209115	0	19	theme	Cellulose	42:50	arg1	Composites					52:61	Novel Cellulose Composites	36:61	Novel Cellulose Composites	36:61	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	5	20	theme	negative	905:912	arg1	bacteria					944:951	Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria	847:951	Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria	847:951	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	0	21	theme	Novel	36:40	arg1	Composites					52:61	Novel Cellulose Composites	36:61	Novel Cellulose Composites	36:61	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	1	22	theme	ferrite-lignin	348:361	arg1	hybrid					363:368	ferrite-lignin hybrid	348:368	ferrite-lignin hybrid	348:368	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	5	23	theme	positive	852:859	arg1	bacteria					944:951	Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria	847:951	Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria	847:951	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	5	24	theme	Staphylococcus	862:875	arg1	25923					889:893	Staphylococcus aureus ATCC 25923	862:893	Staphylococcus aureus ATCC 25923	862:893	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	5	25	dep	Escherichia	915:925	arg1	coli					927:930	coli	927:930	coli	927:930	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	3	26	theme	studied	532:538	arg1	formulations					540:551	the studied formulations	528:551	the studied formulations	528:551	The nature of the filler used in the studied formulations influenced both bioadhesion and mucoadhesion parameters.					
34209115	0	27	theme	Composites	52:61	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	0	27	theme	Composites	52:61	arg1	Development					0:10	Development	0:10	Development	0:10	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	2	28	theme	mechanical	392:401	arg1	performances					403:414	The mechanical performances	388:414	The mechanical performances (Young's modulus and compressive strength)	388:457	The mechanical performances (Young's modulus and compressive strength) increased with the filler addition.					
34209115	5	29	dep	negative	905:912	arg1	25922					937:941	Escherichia coli ATCC 25922	915:941	Escherichia coli ATCC 25922	915:941	All materials exhibited activity against Gram positive (Staphylococcus aureus ATCC 25923) and Gram negative (Escherichia coli ATCC 25922) bacteria.					
34209115	4	30	theme	hybrid	675:680	arg1	incorporation					632:644	the incorporation	628:644	the incorporation of lignin and ferrite-lignin hybrid into the matrix	628:696	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	4	31	theme	ferrite-lignin	660:673	arg1	hybrid					675:680	lignin and ferrite-lignin hybrid	649:680	hybrid	675:680	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	0	32	theme	1-Ethyl-3-methylimidazolium	75:101	arg1	Chloride					103:110	1-Ethyl-3-methylimidazolium Chloride	75:110	1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems	75:140	Development and Characterization of Novel Cellulose Composites Obtained in 1-Ethyl-3-methylimidazolium Chloride Used as Drug Delivery Systems.					
34209115	1	33	theme	1-ethyl-3-methylimidazolium	218:244	arg1	chloride					246:253	1-ethyl-3-methylimidazolium chloride	218:253	1-ethyl-3-methylimidazolium chloride	218:253	Two polysaccharides (cellulose and chitosan) and polyurethane dissolved in 1-ethyl-3-methylimidazolium chloride represented the matrix for the obtainment of new composite formulations comprised of lignin, ferrite-lignin hybrid and ketoconazole.					
34209115	4	34	theme	release	747:753	arg1	rate					726:729	the in vitro rate	713:729	the in vitro rate	713:729	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	4	35	theme	lignin	649:654	arg1	hybrid					675:680	lignin and ferrite-lignin hybrid	649:680	hybrid	675:680	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	4	36	dep	in	717:718	arg1	vitro					720:724	vitro	720:724	vitro	720:724	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34209115	4	37	theme	ketoconazole	734:745	arg1	release					747:753	ketoconazole release	734:753	ketoconazole release	734:753	It was found that the incorporation of lignin and ferrite-lignin hybrid into the matrix has influenced the in vitro rate of ketoconazole release, which is described by the Korsmeyer-Peppas model.					
34627321	8	0	theme	Antibacterial	953:965	arg1	effects					967:973	Antibacterial effects	953:973	Antibacterial effects against E. faecalis biofilms	953:1002	Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples.					
34627321	3	1	theme	antibacterial	484:496	arg1	applications					498:509	antibacterial applications	484:509	antibacterial applications	484:509	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	5	2	theme	paste	666:670	arg1	properties					647:656	The antibacterial properties	629:656	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs)	629:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	10	3	contain	had	1200:1202	arg1	CPC-chitosan-GO					1179:1193	CPC-chitosan-GO	1179:1193	CPC-chitosan-GO	1179:1193	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	10	3	contain	had	1200:1202	arg2	activity					1226:1233	greater antibacterial activity	1204:1233	greater antibacterial activity	1204:1233	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	9	4	theme	hDPSC	1115:1119	arg1	support					1121:1127	excellent hDPSC support	1105:1127	excellent hDPSC support	1105:1127	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	2	5	theme	bone	277:280	arg1	scaffold					295:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	5	6	theme	antibacterial	633:645	arg1	properties					647:656	The antibacterial properties	629:656	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs)	629:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	7	7	dep	hDPSC	875:879	arg1	hDPSC					875:879	The hDPSC growth and viability	871:900	The hDPSC growth and viability on scaffolds	871:913	The hDPSC growth and viability on scaffolds were investigated by live/dead assay.					
34627321	7	7	dep	hDPSC	875:879	arg1	viability					892:900	viability	892:900	viability	892:900	The hDPSC growth and viability on scaffolds were investigated by live/dead assay.					
34627321	7	7	dep	hDPSC	875:879	arg1	growth					881:886	growth	881:886	growth	881:886	The hDPSC growth and viability on scaffolds were investigated by live/dead assay.					
34627321	11	8	dep	E.	1403:1404	arg1	faecalis					1406:1413	faecalis	1406:1413	faecalis	1406:1413	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	8	9	theme	E.	983:984	arg1	biofilms					995:1002	E. faecalis biofilms	983:1002	E. faecalis biofilms	983:1002	Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples.					
34627321	11	10	theme	CPC-chitosan-GO	1348:1362	arg1	paste					1364:1368	The injectable CPC-chitosan-GO paste	1333:1368	The injectable CPC-chitosan-GO paste	1333:1368	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	10	11	theme	antibacterial	1212:1224	arg1	activity					1226:1233	greater antibacterial activity	1204:1233	greater antibacterial activity	1204:1233	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	4	12	contain	containing	567:576	arg1	paste					561:565	an injectable CPC-chitosan paste	534:565	an injectable CPC-chitosan paste containing GO	534:579	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	4	12	contain	containing	567:576	arg2	GO					578:579	GO	578:579	GO	578:579	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	1	13	theme	Enterococcus	137:148	arg1	faecalis					150:157	BACKGROUND Enterococcus faecalis	126:157	BACKGROUND Enterococcus faecalis (E. faecalis)	126:171	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	1	13	theme	Enterococcus	137:148	arg1	species					195:201	the most recovered species	176:201	the most recovered species from the root canals	176:222	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	11	14	theme	in	1505:1506	arg1	shaping					1497:1503	shaping	1497:1503	shaping in situ	1497:1511	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	2	15	theme	Calcium	259:265	arg1	scaffold					295:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	11	16	contain	had	1370:1372	arg1	paste					1364:1368	The injectable CPC-chitosan-GO paste	1333:1368	The injectable CPC-chitosan-GO paste	1333:1368	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	11	16	contain	had	1370:1372	arg2	effects					1381:1387	strong effects	1374:1387	strong effects	1374:1387	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	5	17	theme	stem	746:749	arg1	hDPSCs					758:763	hDPSCs	758:763	hDPSCs	758:763	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	5	17	theme	stem	746:749	arg1	cells					751:755	human dental pulp stem cells	728:755	human dental pulp stem cells (hDPSCs)	728:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	11	18	theme	endodontic	1527:1536	arg1	therapy					1538:1544	promising endodontic therapy	1517:1544	promising endodontic therapy	1517:1544	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	9	19	contain	had	1101:1103	arg1	disks					1095:1099	The antibacterial CPC-chitosan-GO disks	1061:1099	The antibacterial CPC-chitosan-GO disks	1061:1099	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	9	19	contain	had	1101:1103	arg2	support					1121:1127	excellent hDPSC support	1105:1127	excellent hDPSC support	1105:1127	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	8	20	theme	detin	1032:1036	arg1	samples					1044:1050	clinical detin block samples	1023:1050	clinical detin block samples	1023:1050	Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples.					
34627321	1	21	dep	faecalis	150:157	arg1	faecalis					163:170	E. faecalis	160:170	E. faecalis	160:170	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	9	22	theme	CPC-chitosan-GO	1079:1093	arg1	disks					1095:1099	The antibacterial CPC-chitosan-GO disks	1061:1099	The antibacterial CPC-chitosan-GO disks	1061:1099	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	0	23	theme	pulp	109:112	arg1	cells					119:123	dental pulp stem cells	102:123	dental pulp stem cells	102:123	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	11	24	dep	in	1505:1506	arg1	situ					1508:1511	situ	1508:1511	situ	1508:1511	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	7	25	from	hDPSC	875:879	arg1	scaffolds					905:913	scaffolds	905:913	scaffolds	905:913	The hDPSC growth and viability on scaffolds were investigated by live/dead assay.					
34627321	2	26	theme	sealer	382:387	arg1	kind					363:366	a kind	361:366	a kind of root canal sealer	361:387	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	5	27	theme	dental	734:739	arg1	hDPSCs					758:763	hDPSCs	758:763	hDPSCs	758:763	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	5	27	theme	dental	734:739	arg1	cells					751:755	human dental pulp stem cells	728:755	human dental pulp stem cells (hDPSCs)	728:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	3	28	dep	oxide	399:403	arg1	GO					406:407	GO	406:407	GO	406:407	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	10	29	theme	block	1296:1300	arg1	p < 0.05					1310:1317	p < 0.05	1310:1317	p < 0.05	1310:1317	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	10	29	theme	block	1296:1300	arg1	models					1302:1307	detin block models	1290:1307	detin block models (p < 0.05)	1290:1318	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	2	30	theme	root	371:374	arg1	sealer					382:387	root canal sealer	371:387	root canal sealer	371:387	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	0	31	theme	Novel	0:4	arg1	cement					43:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	0	32	theme	oxide-calcium	19:31	arg1	cement					43:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	4	33	theme	injectable	537:546	arg1	paste					561:565	an injectable CPC-chitosan paste	534:565	an injectable CPC-chitosan paste containing GO	534:579	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	0	34	dep	Enterococcus	59:70	arg1	faecalis					72:79	faecalis	72:79	faecalis	72:79	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	11	35	dep	CONCLUSIONS	1321:1331	arg1	had					1370:1372	had	1370:1372	had	1370:1372	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	9	36	theme	live	1153:1156	arg1	cells					1158:1162	live cells	1153:1162	live cells	1153:1162	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	2	37	theme	endodontic	337:346	arg1	treatment					348:356	endodontic treatment	337:356	endodontic treatment as a kind of root canal sealer	337:387	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	0	38	theme	Enterococcus	59:70	arg1	biofilm					81:87	Enterococcus faecalis biofilm	59:87	Enterococcus faecalis biofilm	59:87	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	6	39	theme	GO	832:833	arg1	scaffold					846:853	GO injectable scaffold	832:853	GO injectable scaffold	832:853	METHODS CPC-chitosan composite with or without GO injectable scaffold was fabricated.					
34627321	4	40	theme	present	519:525	arg1	study					527:531	the present study	515:531	the present study	515:531	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	10	41	theme	greater	1204:1210	arg1	activity					1226:1233	greater antibacterial activity	1204:1233	greater antibacterial activity	1204:1233	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	10	42	theme	detin	1290:1294	arg1	p < 0.05					1310:1317	p < 0.05	1310:1317	p < 0.05	1310:1317	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	10	42	theme	detin	1290:1294	arg1	models					1302:1307	detin block models	1290:1307	detin block models (p < 0.05)	1290:1318	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	1	43	theme	root	237:240	arg1	treatment					248:256	failed root canal treatment	230:256	failed root canal treatment	230:256	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	4	44	theme	promising	599:607	arg1	therapy					620:626	promising endodontic therapy	599:626	promising endodontic therapy	599:626	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	11	45	theme	E.	1403:1404	arg1	inhibition					1392:1401	inhibition	1392:1401	inhibition E. faecalis	1392:1413	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	2	46	from	applications	321:332	arg1	treatment					348:356	endodontic treatment	337:356	endodontic treatment as a kind of root canal sealer	337:387	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	6	47	theme	CPC-chitosan	793:804	arg1	composite					806:814	CPC-chitosan composite	793:814	CPC-chitosan composite	793:814	METHODS CPC-chitosan composite with or without GO injectable scaffold was fabricated.					
34627321	2	48	theme	CPC	290:292	arg1	scaffold					295:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	11	49	dep	had	1370:1372	arg1	fill					1446:1449	fill	1446:1449	fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy	1446:1544	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	3	50	theme	promising	455:463	arg1	nano-materials					465:478	promising nano-materials	455:478	promising nano-materials for antibacterial applications	455:509	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	2	51	theme	cement	282:287	arg1	scaffold					295:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	6	52	with	METHODS	785:791	arg1	composite					806:814	CPC-chitosan composite	793:814	CPC-chitosan composite	793:814	METHODS CPC-chitosan composite with or without GO injectable scaffold was fabricated.					
34627321	1	53	theme	recovered	185:193	arg1	faecalis					150:157	BACKGROUND Enterococcus faecalis	126:157	BACKGROUND Enterococcus faecalis (E. faecalis)	126:171	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	1	53	theme	recovered	185:193	arg1	species					195:201	the most recovered species	176:201	the most recovered species from the root canals	176:222	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	8	54	theme	clinical	1023:1030	arg1	samples					1044:1050	clinical detin block samples	1023:1050	clinical detin block samples	1023:1050	Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples.					
34627321	11	55	theme	injectable	1337:1346	arg1	paste					1364:1368	The injectable CPC-chitosan-GO paste	1333:1368	The injectable CPC-chitosan-GO paste	1333:1368	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	9	56	dep	RESULTS	1053:1059	arg1	had					1101:1103	had	1101:1103	had excellent hDPSC support with the percentages of live cells at around 90%	1101:1176	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	1	57	theme	root	212:215	arg1	canals					217:222	the root canals	208:222	the root canals	208:222	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	11	58	dep	fill	1446:1449	arg1	could					1440:1444	could	1440:1444	could	1440:1444	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	11	59	theme	strong	1374:1379	arg1	effects					1381:1387	strong effects	1374:1387	strong effects	1374:1387	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	1	60	from	canals	217:222	arg1	faecalis					150:157	BACKGROUND Enterococcus faecalis	126:157	BACKGROUND Enterococcus faecalis (E. faecalis)	126:171	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	1	60	from	canals	217:222	arg1	species					195:201	the most recovered species	176:201	the most recovered species from the root canals	176:222	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	1	61	theme	BACKGROUND	126:135	arg1	faecalis					150:157	BACKGROUND Enterococcus faecalis	126:157	BACKGROUND Enterococcus faecalis (E. faecalis)	126:171	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	1	61	theme	BACKGROUND	126:135	arg1	species					195:201	the most recovered species	176:201	the most recovered species from the root canals	176:222	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	5	62	theme	faecalis	683:690	arg1	biofilms					692:699	E. faecalis biofilms	680:699	E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs)	680:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	2	63	theme	phosphate	267:275	arg1	scaffold					295:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold	259:302	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	5	64	theme	pulp	741:744	arg1	hDPSCs					758:763	hDPSCs	758:763	hDPSCs	758:763	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	5	64	theme	pulp	741:744	arg1	cells					751:755	human dental pulp stem cells	728:755	human dental pulp stem cells (hDPSCs)	728:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	10	65	theme	CPC-chitosan	1263:1274	arg1	control					1276:1282	CPC-chitosan control	1263:1282	CPC-chitosan control using detin block models (p < 0.05)	1263:1318	CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05).					
34627321	11	66	theme	promising	1517:1525	arg1	therapy					1538:1544	promising endodontic therapy	1517:1544	promising endodontic therapy	1517:1544	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	0	67	theme	dental	102:107	arg1	cells					119:123	dental pulp stem cells	102:123	dental pulp stem cells	102:123	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	8	68	theme	block	1038:1042	arg1	samples					1044:1050	clinical detin block samples	1023:1050	clinical detin block samples	1023:1050	Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples.					
34627321	9	69	theme	antibacterial	1065:1077	arg1	disks					1095:1099	The antibacterial CPC-chitosan-GO disks	1061:1099	The antibacterial CPC-chitosan-GO disks	1061:1099	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	0	70	theme	stem	114:117	arg1	cells					119:123	dental pulp stem cells	102:123	dental pulp stem cells	102:123	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	5	71	theme	human	728:732	arg1	hDPSCs					758:763	hDPSCs	758:763	hDPSCs	758:763	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	5	71	theme	human	728:732	arg1	cells					751:755	human dental pulp stem cells	728:755	human dental pulp stem cells (hDPSCs)	728:764	The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated.					
34627321	9	72	theme	excellent	1105:1113	arg1	support					1121:1127	excellent hDPSC support	1105:1127	excellent hDPSC support	1105:1127	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	2	73	theme	canal	376:380	arg1	sealer					382:387	root canal sealer	371:387	root canal sealer	371:387	Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer.					
34627321	0	74	theme	nanographene	6:17	arg1	cement					43:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	7	75	theme	live/dead	936:944	arg1	assay					946:950	live/dead assay	936:950	live/dead assay	936:950	The hDPSC growth and viability on scaffolds were investigated by live/dead assay.					
34627321	0	76	theme	phosphate	33:41	arg1	cement					43:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement	0:48	Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells.					
34627321	4	77	theme	CPC-chitosan	548:559	arg1	paste					561:565	an injectable CPC-chitosan paste	534:565	an injectable CPC-chitosan paste containing GO	534:579	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	8	78	dep	E.	983:984	arg1	faecalis					986:993	faecalis	986:993	faecalis	986:993	Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples.					
34627321	6	79	theme	injectable	835:844	arg1	scaffold					846:853	GO injectable scaffold	832:853	GO injectable scaffold	832:853	METHODS CPC-chitosan composite with or without GO injectable scaffold was fabricated.					
34627321	3	80	theme	Graphene	390:397	arg1	kind					447:450	a kind	445:450	a kind of promising nano-materials for antibacterial applications	445:509	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	3	80	theme	Graphene	390:397	arg1	oxide					399:403	Graphene oxide	390:403	Graphene oxide (GO)	390:408	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	9	81	theme	cells	1158:1162	arg1	percentages					1138:1148	the percentages	1134:1148	the percentages of live cells	1134:1162	RESULTS The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%.					
34627321	3	82	theme	nano-materials	465:478	arg1	kind					447:450	a kind	445:450	a kind of promising nano-materials for antibacterial applications	445:509	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	3	82	theme	nano-materials	465:478	arg1	oxide					399:403	Graphene oxide	390:403	Graphene oxide (GO)	390:408	Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications.					
34627321	1	83	theme	failed	230:235	arg1	treatment					248:256	failed root canal treatment	230:256	failed root canal treatment	230:256	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
34627321	4	84	theme	endodontic	609:618	arg1	therapy					620:626	promising endodontic therapy	599:626	promising endodontic therapy	599:626	In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy.					
34627321	11	85	theme	hDPSC	1419:1423	arg1	support					1425:1431	hDPSC support	1419:1431	hDPSC support	1419:1431	CONCLUSIONS The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.					
34627321	1	86	theme	canal	242:246	arg1	treatment					248:256	failed root canal treatment	230:256	failed root canal treatment	230:256	BACKGROUND Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment.					
32853381	5	0	theme	Rivaroxaban	820:830	arg1	users					841:845	Rivaroxaban and LMWH users	820:845	Rivaroxaban and LMWH users	820:845	Rivaroxaban and LMWH users were 1:1 propensity score matched.					
32853381	10	1	dep	HR	1402:1403	arg1	0.71					1406:1409	0.71	1406:1409	0.71	1406:1409	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	1	dep	HR	1402:1403	arg1	0.41-1.22					1420:1428	0.41-1.22	1420:1428	0.41-1.22	1420:1428	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	1	dep	HR	1402:1403	arg1	CI					1416:1417	95% CI	1412:1417	95% CI	1412:1417	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	2	theme	LMWH	1524:1527	arg1	risk					1371:1374	risk	1371:1374	risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH	1371:1527	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	3	theme	outcome	1393:1399	arg1	risk					1371:1374	risk	1371:1374	risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH	1371:1527	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	1	4	theme	low-molecular-weight	213:232	arg1	LMWH					243:246	LMWH	243:246	LMWH	243:246	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	1	4	theme	low-molecular-weight	213:232	arg1	heparin					234:240	low-molecular-weight heparin	213:240	low-molecular-weight heparin (LMWH)	213:247	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	11	5	theme	similar	1639:1645	arg1	results					1647:1653	similar results	1639:1653	similar results	1639:1653	On-treatment analysis at 12 months showed similar results.					
32853381	0	6	theme	cancer-associated	86:102	arg1	thromboembolism					104:118	cancer-associated thromboembolism	86:118	cancer-associated thromboembolism	86:118	Effectiveness and safety of rivaroxaban compared with low-molecular-weight heparin in cancer-associated thromboembolism.					
32853381	12	7	theme	reasonable	1677:1686	arg1	alternative					1688:1698	a reasonable alternative	1675:1698	a reasonable alternative to LMWH for patients with CAT without gastrointestinal or genitourinary cancer	1675:1777	Rivaroxaban may be a reasonable alternative to LMWH for patients with CAT without gastrointestinal or genitourinary cancer.					
32853381	12	7	theme	reasonable	1677:1686	arg1	Rivaroxaban					1656:1666	Rivaroxaban	1656:1666	Rivaroxaban	1656:1666	Rivaroxaban may be a reasonable alternative to LMWH for patients with CAT without gastrointestinal or genitourinary cancer.					
32853381	10	8	theme	bleeding	1438:1445	arg1	risk					1371:1374	risk	1371:1374	risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH	1371:1527	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	9	theme	%	1414:1414	arg1	CI					1416:1417	95% CI	1412:1417	95% CI	1412:1417	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	9	10	theme	529	1284:1286	arg1	patients					1301:1308	529 rivaroxaban- and 529 LMWH-treated patients	1263:1308	529 rivaroxaban- and 529 LMWH-treated patients with CAT	1263:1317	We included 529 rivaroxaban- and 529 LMWH-treated patients with CAT.					
32853381	10	11	theme	%	1460:1460	arg1	CI					1462:1463	95% CI	1458:1463	95% CI	1458:1463	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	12	12	with	patients	1712:1719	arg1	CAT					1726:1728	CAT	1726:1728	CAT	1726:1728	Rivaroxaban may be a reasonable alternative to LMWH for patients with CAT without gastrointestinal or genitourinary cancer.					
32853381	8	13	used	used	1177:1180	arg2	models					1123:1128	Proportional hazards models	1102:1128	Proportional hazards models for the subdistribution of competing risk	1102:1170	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	3	14	theme	outpatient	713:722	arg1	anticoagulation					724:738	outpatient anticoagulation	713:738	outpatient anticoagulation	713:738	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	8	15	theme	Proportional	1102:1113	arg1	models					1123:1128	Proportional hazards models	1102:1128	Proportional hazards models for the subdistribution of competing risk	1102:1170	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	4	16	theme	genitourinary	783:795	arg1	cancers					797:803	luminal gastrointestinal or genitourinary cancers	755:803	luminal gastrointestinal or genitourinary cancers	755:803	Patients with luminal gastrointestinal or genitourinary cancers were excluded.					
32853381	8	17	with	ratios	1201:1206	arg1	CIs					1245:1247	CIs	1245:1247	CIs	1245:1247	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	8	17	with	ratios	1201:1206	arg1	intervals					1234:1242	95% confidence intervals	1219:1242	95% confidence intervals (CIs)	1219:1248	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	2	18	dep	effectiveness	344:356	arg1	the					340:342	the	340:342	the	340:342	This study was undertaken to evaluate the effectiveness and safety of rivaroxaban vs LMWH for treatment of CAT.					
32853381	3	19	theme	active	536:541	arg1	breast					543:548	active breast	536:548	active breast	536:548	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	8	20	theme	%	1221:1221	arg1	CIs					1245:1247	CIs	1245:1247	CIs	1245:1247	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	8	20	theme	%	1221:1221	arg1	intervals					1234:1242	95% confidence intervals	1219:1242	95% confidence intervals (CIs)	1219:1248	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	12	21	theme	genitourinary	1758:1770	arg1	cancer					1772:1777	gastrointestinal or genitourinary cancer	1738:1777	gastrointestinal or genitourinary cancer	1738:1777	Rivaroxaban may be a reasonable alternative to LMWH for patients with CAT without gastrointestinal or genitourinary cancer.					
32853381	5	22	theme	1:1	852:854	arg1	score					867:871	1:1 propensity score	852:871	1:1 propensity score	852:871	Rivaroxaban and LMWH users were 1:1 propensity score matched.					
32853381	5	23	theme	LMWH	836:839	arg1	users					841:845	Rivaroxaban and LMWH users	820:845	Rivaroxaban and LMWH users	820:845	Rivaroxaban and LMWH users were 1:1 propensity score matched.					
32853381	10	24	theme	95	1458:1459	arg1	%					1460:1460	%	1460:1460	%	1460:1460	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	3	25	theme	pancreatic	569:578	arg1	cancer					580:585	pancreatic cancer	569:585	pancreatic cancer	569:585	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	10	26	theme	recurrent	1545:1553	arg1	thrombosis					1555:1564	recurrent thrombosis	1545:1564	recurrent thrombosis	1545:1564	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	5	27	theme	propensity	856:865	arg1	score					867:871	1:1 propensity score	852:871	1:1 propensity score	852:871	Rivaroxaban and LMWH users were 1:1 propensity score matched.					
32853381	10	28	theme	mortality	1481:1489	arg1	risk					1371:1374	risk	1371:1374	risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH	1371:1527	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	2	29	theme	rivaroxaban	372:382	arg1	safety					362:367	safety	362:367	safety	362:367	This study was undertaken to evaluate the effectiveness and safety of rivaroxaban vs LMWH for treatment of CAT.					
32853381	2	29	theme	rivaroxaban	372:382	arg1	effectiveness					344:356	effectiveness	344:356	effectiveness	344:356	This study was undertaken to evaluate the effectiveness and safety of rivaroxaban vs LMWH for treatment of CAT.					
32853381	9	30	with	patients	1301:1308	arg1	CAT					1315:1317	CAT	1315:1317	CAT	1315:1317	We included 529 rivaroxaban- and 529 LMWH-treated patients with CAT.					
32853381	3	31	theme	Epidemiology	437:448	arg1	data					482:485	US Surveillance, Epidemiology and End Results-Medicare-linked data	420:485	US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016	420:508	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	6	32	theme	intent-to-treat	1018:1032	arg1	approach					1034:1041	an intent-to-treat approach	1015:1041	an intent-to-treat approach	1015:1041	Outcomes included the composite of recurrent thrombosis or major bleeding, each outcome separately, and mortality at 6 months, using an intent-to-treat approach.					
32853381	4	33	theme	luminal	755:761	arg1	cancers					797:803	luminal gastrointestinal or genitourinary cancers	755:803	luminal gastrointestinal or genitourinary cancers	755:803	Patients with luminal gastrointestinal or genitourinary cancers were excluded.					
32853381	9	34	theme	rivaroxaban-	1267:1278	arg1	patients					1301:1308	529 rivaroxaban- and 529 LMWH-treated patients	1263:1308	529 rivaroxaban- and 529 LMWH-treated patients with CAT	1263:1317	We included 529 rivaroxaban- and 529 LMWH-treated patients with CAT.					
32853381	3	35	theme	US	420:421	arg1	Surveillance					423:434	US Surveillance	420:434	US Surveillance	420:434	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	8	36	theme	95	1219:1220	arg1	%					1221:1221	%	1221:1221	%	1221:1221	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	11	37	from	months	1625:1630	arg1	analysis					1610:1617	On-treatment analysis	1597:1617	On-treatment analysis at 12 months	1597:1630	On-treatment analysis at 12 months showed similar results.					
32853381	10	38	theme	0.87	1496:1499	arg1	HR					1492:1493	HR	1492:1493	HR	1492:1493	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	38	theme	0.87	1496:1499	arg1	mortality					1481:1489	mortality	1481:1489	mortality (HR, 0.87; 95% CI, 0.70-1.07)	1481:1519	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	12	39	theme	gastrointestinal	1738:1753	arg1	cancer					1772:1777	gastrointestinal or genitourinary cancer	1738:1777	gastrointestinal or genitourinary cancer	1738:1777	Rivaroxaban may be a reasonable alternative to LMWH for patients with CAT without gastrointestinal or genitourinary cancer.					
32853381	3	40	theme	Surveillance	423:434	arg1	data					482:485	US Surveillance, Epidemiology and End Results-Medicare-linked data	420:485	US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016	420:508	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	4	41	with	Patients	741:748	arg1	cancers					797:803	luminal gastrointestinal or genitourinary cancers	755:803	luminal gastrointestinal or genitourinary cancers	755:803	Patients with luminal gastrointestinal or genitourinary cancers were excluded.					
32853381	1	42	theme	differing	140:148	arg1	recommendations					150:164	differing recommendations	140:164	differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT)	140:299	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	3	43	theme	Results-Medicare-linked	458:480	arg1	data					482:485	US Surveillance, Epidemiology and End Results-Medicare-linked data	420:485	US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016	420:508	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	8	44	theme	risk	1167:1170	arg1	subdistribution					1138:1152	the subdistribution	1134:1152	the subdistribution of competing risk	1134:1170	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	6	45	theme	thrombosis	927:936	arg1	mortality					986:994	mortality	986:994	mortality	986:994	Outcomes included the composite of recurrent thrombosis or major bleeding, each outcome separately, and mortality at 6 months, using an intent-to-treat approach.					
32853381	6	45	theme	thrombosis	927:936	arg1	composite					904:912	the composite	900:912	the composite of recurrent thrombosis	900:936	Outcomes included the composite of recurrent thrombosis or major bleeding, each outcome separately, and mortality at 6 months, using an intent-to-treat approach.					
32853381	6	45	theme	thrombosis	927:936	arg1	thrombosis					927:936	recurrent thrombosis	917:936	recurrent thrombosis	917:936	Outcomes included the composite of recurrent thrombosis or major bleeding, each outcome separately, and mortality at 6 months, using an intent-to-treat approach.					
32853381	6	45	theme	thrombosis	927:936	arg1	outcome					962:968	each outcome	957:968	each outcome separately	957:979	Outcomes included the composite of recurrent thrombosis or major bleeding, each outcome separately, and mortality at 6 months, using an intent-to-treat approach.					
32853381	10	46	dep	HR	1567:1568	arg1	%					1579:1579	95% CI	1577:1582	95% CI	1577:1582	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	46	dep	HR	1567:1568	arg1	0.15-0.95					1585:1593	0.15-0.95	1585:1593	0.15-0.95	1585:1593	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	46	dep	HR	1567:1568	arg1	0.37					1571:1574	0.37	1571:1574	0.37	1571:1574	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	1	47	theme	cancer-associated	266:282	arg1	CAT					296:298	CAT	296:298	CAT	296:298	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	1	47	theme	cancer-associated	266:282	arg1	thrombosis					284:293	cancer-associated thrombosis	266:293	cancer-associated thrombosis (CAT)	266:299	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	0	48	theme	rivaroxaban	28:38	arg1	safety					18:23	safety	18:23	safety	18:23	Effectiveness and safety of rivaroxaban compared with low-molecular-weight heparin in cancer-associated thromboembolism.					
32853381	0	48	theme	rivaroxaban	28:38	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness	0:12	Effectiveness and safety of rivaroxaban compared with low-molecular-weight heparin in cancer-associated thromboembolism.					
32853381	3	49	with	adults	524:529	arg1	breast					543:548	active breast	536:548	active breast	536:548	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	3	49	with	adults	524:529	arg1	cancer					580:585	pancreatic cancer	569:585	pancreatic cancer	569:585	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	3	49	with	adults	524:529	arg1	ovarian					557:563	ovarian	557:563	ovarian	557:563	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	3	49	with	adults	524:529	arg1	lung					551:554	lung	551:554	lung	551:554	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	6	50	theme	recurrent	917:925	arg1	thrombosis					927:936	recurrent thrombosis	917:936	recurrent thrombosis	917:936	Outcomes included the composite of recurrent thrombosis or major bleeding, each outcome separately, and mortality at 6 months, using an intent-to-treat approach.					
32853381	10	51	theme	composite	1383:1391	arg1	HR					1402:1403	HR	1402:1403	HR	1402:1403	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	51	theme	composite	1383:1391	arg1	outcome					1393:1399	the composite outcome	1379:1399	the composite outcome (HR, 0.71; 95% CI, 0.41-1.22)	1379:1429	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	1	52	theme	thrombosis	284:293	arg1	treatment					253:261	treatment	253:261	treatment of cancer-associated thrombosis (CAT)	253:299	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	0	53	theme	low-molecular-weight	54:73	arg1	heparin					75:81	low-molecular-weight heparin	54:81	low-molecular-weight heparin in cancer-associated thromboembolism	54:118	Effectiveness and safety of rivaroxaban compared with low-molecular-weight heparin in cancer-associated thromboembolism.					
32853381	0	54	from	heparin	75:81	arg1	thromboembolism					104:118	cancer-associated thromboembolism	86:118	cancer-associated thromboembolism	86:118	Effectiveness and safety of rivaroxaban compared with low-molecular-weight heparin in cancer-associated thromboembolism.					
32853381	3	55	link	Results-Medicare-linked	458:480	arg1	data					482:485	US Surveillance, Epidemiology and End Results-Medicare-linked data	420:485	US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016	420:508	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	3	56	theme	End	454:456	arg1	data					482:485	US Surveillance, Epidemiology and End Results-Medicare-linked data	420:485	US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016	420:508	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	10	57	dep	HR	1448:1449	arg1	CI					1462:1463	95% CI	1458:1463	95% CI	1458:1463	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	57	dep	HR	1448:1449	arg1	0.50-2.01					1466:1474	0.50-2.01	1466:1474	0.50-2.01	1466:1474	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	9	58	theme	LMWH-treated	1288:1299	arg1	patients					1301:1308	529 rivaroxaban- and 529 LMWH-treated patients	1263:1308	529 rivaroxaban- and 529 LMWH-treated patients with CAT	1263:1317	We included 529 rivaroxaban- and 529 LMWH-treated patients with CAT.					
32853381	8	59	theme	hazards	1115:1121	arg1	models					1123:1128	Proportional hazards models	1102:1128	Proportional hazards models for the subdistribution of competing risk	1102:1170	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	4	60	theme	gastrointestinal	763:778	arg1	cancers					797:803	luminal gastrointestinal or genitourinary cancers	755:803	luminal gastrointestinal or genitourinary cancers	755:803	Patients with luminal gastrointestinal or genitourinary cancers were excluded.					
32853381	10	61	dep	reduced	1537:1543	arg1	HR					1567:1568	HR	1567:1568	HR	1567:1568	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	3	62	theme	emergency	640:648	arg1	department					650:659	the emergency department	636:659	the emergency department	636:659	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	1	63	theme	direct-acting	176:188	arg1	anticoagulants					195:208	direct-acting oral anticoagulants	176:208	direct-acting oral anticoagulants	176:208	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	10	64	dep	HR	1492:1493	arg1	0.70-1.07					1510:1518	0.70-1.07	1510:1518	0.70-1.07	1510:1518	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	10	64	dep	HR	1492:1493	arg1	%					1504:1504	95% CI	1502:1507	95% CI	1502:1507	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	8	65	theme	confidence	1223:1232	arg1	CIs					1245:1247	CIs	1245:1247	CIs	1245:1247	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	8	65	theme	confidence	1223:1232	arg1	intervals					1234:1242	95% confidence intervals	1219:1242	95% confidence intervals (CIs)	1219:1248	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	7	66	theme	On-treatment	1044:1055	arg1	analysis					1057:1064	On-treatment analysis	1044:1064	On-treatment analysis after 12 months	1044:1080	On-treatment analysis after 12 months was also performed.					
32853381	1	67	theme	oral	190:193	arg1	anticoagulants					195:208	direct-acting oral anticoagulants	176:208	direct-acting oral anticoagulants	176:208	Guidelines provide differing recommendations regarding direct-acting oral anticoagulants vs low-molecular-weight heparin (LMWH) for treatment of cancer-associated thrombosis (CAT).					
32853381	2	68	theme	LMWH	387:390	arg1	safety					362:367	safety	362:367	safety	362:367	This study was undertaken to evaluate the effectiveness and safety of rivaroxaban vs LMWH for treatment of CAT.					
32853381	2	68	theme	LMWH	387:390	arg1	effectiveness					344:356	effectiveness	344:356	effectiveness	344:356	This study was undertaken to evaluate the effectiveness and safety of rivaroxaban vs LMWH for treatment of CAT.					
32853381	3	69	from	2013	492:495	arg1	data					482:485	US Surveillance, Epidemiology and End Results-Medicare-linked data	420:485	US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016	420:508	Using US Surveillance, Epidemiology and End Results-Medicare-linked data from 2013 through 2016, we evaluated adults with active breast, lung, ovarian, or pancreatic cancer, who were admitted to the hospital or treated in the emergency department for CAT and were prescribed rivaroxaban or LMWH for outpatient anticoagulation.					
32853381	2	70	theme	CAT	409:411	arg1	treatment					396:404	treatment	396:404	treatment of CAT	396:411	This study was undertaken to evaluate the effectiveness and safety of rivaroxaban vs LMWH for treatment of CAT.					
32853381	11	71	theme	On-treatment	1597:1608	arg1	analysis					1610:1617	On-treatment analysis	1597:1617	On-treatment analysis at 12 months	1597:1630	On-treatment analysis at 12 months showed similar results.					
32853381	8	72	theme	hazard	1194:1199	arg1	HRs					1209:1211	HRs	1209:1211	HRs	1209:1211	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	8	72	theme	hazard	1194:1199	arg1	ratios					1201:1206	hazard ratios	1194:1206	hazard ratios (HRs) with 95% confidence intervals (CIs)	1194:1248	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
32853381	10	73	from	differences	1356:1366	arg1	risk					1371:1374	risk	1371:1374	risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH	1371:1527	Rivaroxaban was not associated with differences in risk of the composite outcome (HR, 0.71; 95% CI, 0.41-1.22), major bleeding (HR, 1.01; 95% CI, 0.50-2.01), or mortality (HR, 0.87; 95% CI, 0.70-1.07) vs LMWH, but it reduced recurrent thrombosis (HR, 0.37; 95% CI, 0.15-0.95).					
32853381	8	74	theme	competing	1157:1165	arg1	risk					1167:1170	competing risk	1157:1170	competing risk	1157:1170	Proportional hazards models for the subdistribution of competing risk were used to estimate hazard ratios (HRs) with 95% confidence intervals (CIs).					
34038799	2	0	from	effect	441:446	arg1	terrestris					494:503	Tribulus terrestris L.	485:506	Tribulus terrestris L.	485:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	6	1	theme	ethanol	1175:1181	arg1	concentration					1183:1195	ethanol concentration	1175:1195	ethanol concentration 30%	1175:1199	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	7	2	theme	pulmonary	1543:1551	arg1	edema					1553:1557	pulmonary edema	1543:1557	pulmonary edema	1543:1557	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	9	3	theme	Tribulus	1879:1886	arg1	L.					1899:1900	Tribulus terrestris L.	1879:1900	Tribulus terrestris L.	1879:1900	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	1	4	theme	ETHNOPHARMACOLOGICAL	181:200	arg1	L.					232:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	7	5	theme	stronger	1469:1476	arg1	effect					1496:1501	a stronger anti-inflammatory effect	1467:1501	a stronger anti-inflammatory effect	1467:1501	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	6	6	theme	response	1316:1323	arg1	method					1333:1338	response surface method	1316:1338	response surface method	1316:1338	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	3	7	theme	extraction	595:604	arg1	process					606:612	the extraction process	591:612	the extraction process	591:612	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	8	8	theme	activation	1788:1797	arg1	inhibition					1770:1779	the inhibition	1766:1779	the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway	1766:1840	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	1	9	theme	Tribulus	212:219	arg1	L.					232:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	1	10	from	Zygophyllaceae	265:278	arg1	plant					254:258	an annual herb plant	239:258	an annual herb plant from Zygophyllaceae	239:278	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	4	11	from	L.	890:891	arg1	optimization					809:820	optimization	809:820	optimization of extraction technology of total saponins from Tribulus terrestris L. leaves	809:898	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	4	11	from	L.	890:891	arg1	saponins					856:863	total saponins	850:863	total saponins from Tribulus terrestris L. leaves	850:898	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	4	12	theme	response	767:774	arg1	methods					784:790	The ultrasonic extraction and response surface methods	737:790	methods	784:790	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	6	13	theme	%	1369:1369	arg1	rate					1360:1363	the extracting rate	1345:1363	the extracting rate 5.49%	1345:1369	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	5	14	theme	total	990:994	arg1	saponins					996:1003	total saponins	990:1003	total saponins	990:1003	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	15	theme	extraction	1269:1278	arg1	temperature					1280:1290	extraction temperature 46 °C	1269:1296	extraction temperature 46 °C	1269:1296	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	3	16	theme	anti-inflammatory	633:649	arg1	action					651:656	anti-inflammatory action	633:656	anti-inflammatory action	633:656	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	2	17	from	mechanism	452:460	arg1	terrestris					494:503	Tribulus terrestris L.	485:506	Tribulus terrestris L.	485:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	0	18	theme	HPLC-DAD-ESI-MSn	90:105	arg1	profiling					107:115	their HPLC-DAD-ESI-MSn profiling	84:115	their HPLC-DAD-ESI-MSn profiling	84:115	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	2	19	dep	terrestris	494:503	arg1	L.					505:506	Tribulus terrestris L.	485:506	Tribulus terrestris L.	485:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	4	20	from	optimization	809:820	arg1	L.					890:891	L.	890:891	L.	890:891	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	5	21	theme	induced	1039:1045	arg1	264.7 cells					1051:1061	lipopolysaccharide induced RAW 264.7 cells	1020:1061	lipopolysaccharide induced RAW 264.7 cells	1020:1061	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	4	22	theme	extraction	825:834	arg1	technology					836:845	extraction technology	825:845	extraction technology	825:845	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	6	23	theme	LC-MSn	1415:1420	arg1	method					1422:1427	LC-MSn method	1415:1427	LC-MSn method	1415:1427	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	4	24	dep	MATERIALS	715:723	arg1	extraction					752:761	The ultrasonic extraction and response surface methods	737:790	extraction	752:761	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	4	24	dep	MATERIALS	715:723	arg1	methods					784:790	The ultrasonic extraction and response surface methods	737:790	methods	784:790	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	6	25	theme	fraction	1155:1162	arg1	ratio					1226:1230	ratio	1226:1230	ratio of solvent to material 35:1 ml/g	1226:1263	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	25	theme	fraction	1155:1162	arg1	concentration					1183:1195	ethanol concentration	1175:1195	ethanol concentration 30%	1175:1199	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	25	theme	fraction	1155:1162	arg1	55 min					1218:1223	extraction time 55 min	1202:1223	extraction time 55 min	1202:1223	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	25	theme	fraction	1155:1162	arg1	temperature					1280:1290	extraction temperature 46 °C	1269:1296	extraction temperature 46 °C	1269:1296	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	25	theme	fraction	1155:1162	arg1	RESULTS					1098:1104	RESULTS The ultrasonic extraction parameters	1098:1141	RESULTS The ultrasonic extraction parameters	1098:1141	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	2	26	theme	saponins	471:478	arg1	process					391:397	the extraction process	376:397	the extraction process	376:397	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	26	theme	saponins	471:478	arg1	compositions					409:420	chemical compositions	400:420	chemical compositions	400:420	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	26	theme	saponins	471:478	arg1	effect					441:446	anti-inflammatory effect	423:446	anti-inflammatory effect	423:446	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	26	theme	saponins	471:478	arg1	mechanism					452:460	mechanism	452:460	mechanism	452:460	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	27	from	terrestris	494:503	arg1	saponins					471:478	total saponins	465:478	total saponins from Tribulus terrestris L.	465:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	27	from	terrestris	494:503	arg1	process					391:397	the extraction process	376:397	the extraction process	376:397	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	27	from	terrestris	494:503	arg1	compositions					409:420	chemical compositions	400:420	chemical compositions	400:420	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	27	from	terrestris	494:503	arg1	effect					441:446	anti-inflammatory effect	423:446	anti-inflammatory effect	423:446	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	2	27	from	terrestris	494:503	arg1	mechanism					452:460	mechanism	452:460	mechanism	452:460	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	1	28	theme	many	290:293	arg1	activities					306:315	many biological activities	290:315	many biological activities	290:315	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	3	29	dep	AIM	534:536	arg1	evaluated					581:589	evaluated	581:589	evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L.	581:696	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	9	30	theme	terrestris	1888:1897	arg1	L.					1899:1900	Tribulus terrestris L.	1879:1900	Tribulus terrestris L.	1879:1900	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	0	31	theme	ultrasound	16:25	arg1	extraction					27:36	ultrasound extraction	16:36	ultrasound extraction	16:36	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	7	32	theme	lung	1612:1615	arg1	tissues					1617:1623	lung tissues	1612:1623	lung tissues	1612:1623	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	6	33	theme	extraction	1121:1130	arg1	parameters					1132:1141	The ultrasonic extraction parameters	1106:1141	RESULTS The ultrasonic extraction parameters	1098:1141	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	3	34	theme	terrestris	684:693	arg1	L.					695:696	Tribulus terrestris L.	675:696	Tribulus terrestris L.	675:696	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	2	35	theme	anti-inflammatory	423:439	arg1	effect					441:446	anti-inflammatory effect	423:446	anti-inflammatory effect	423:446	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	7	36	theme	264.7 cells	1596:1606	arg1	morphology					1578:1587	morphology	1578:1587	morphology of RAW 264.7 cells and lung tissues	1578:1623	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	0	37	dep	terrestris	50:59	arg1	L.					61:62	Tribulus terrestris L.	41:62	Tribulus terrestris L.	41:62	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	2	38	theme	Tribulus	485:492	arg1	terrestris					494:503	Tribulus terrestris L.	485:506	Tribulus terrestris L.	485:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	0	39	theme	terrestris	50:59	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasound extraction of Tribulus terrestris L.	0:62	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	1	40	theme	main	326:329	arg1	saponins					357:364	saponins	357:364	saponins	357:364	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	1	40	theme	main	326:329	arg1	constituents					340:351	its main chemical constituents	322:351	its main chemical constituents	322:351	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	2	41	from	compositions	409:420	arg1	terrestris					494:503	Tribulus terrestris L.	485:506	Tribulus terrestris L.	485:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	6	42	theme	extraction	1202:1211	arg1	55 min					1218:1223	extraction time 55 min	1202:1223	extraction time 55 min	1202:1223	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	8	43	theme	total	1701:1705	arg1	extract					1716:1722	total saponins extract	1701:1722	total saponins extract	1701:1722	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	5	44	theme	acute	1067:1071	arg1	injury					1078:1083	acute lung injury	1067:1083	acute lung injury	1067:1083	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	45	theme	%	1199:1199	arg1	concentration					1183:1195	ethanol concentration	1175:1195	ethanol concentration 30%	1175:1199	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	9	46	theme	anti-inflammatory	1938:1954	arg1	saponins					1909:1916	saponins	1909:1916	saponins	1909:1916	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	9	46	theme	anti-inflammatory	1938:1954	arg1	drug					1956:1959	an important anti-inflammatory drug	1925:1959	an important anti-inflammatory drug in clinic	1925:1969	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	4	47	theme	saponins	856:863	arg1	optimization					809:820	optimization	809:820	optimization of extraction technology of total saponins from Tribulus terrestris L. leaves	809:898	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	2	48	theme	extraction	380:389	arg1	process					391:397	the extraction process	376:397	the extraction process	376:397	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	1	49	theme	herb	249:252	arg1	plant					254:258	an annual herb plant	239:258	an annual herb plant from Zygophyllaceae	239:278	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	5	50	theme	injury	1078:1083	arg1	models					1090:1095	lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models	1020:1095	lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models	1020:1095	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	51	theme	5.49	1365:1368	arg1	%					1369:1369	%	1369:1369	%	1369:1369	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	52	theme	extracting	1349:1358	arg1	rate					1360:1363	the extracting rate	1345:1363	the extracting rate 5.49%	1345:1369	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	3	53	theme	present	555:561	arg1	study					563:567	The present study	551:567	The present study	551:567	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	8	54	theme	anti-inflammatory	1738:1754	arg1	action					1756:1761	the anti-inflammatory action	1734:1761	the anti-inflammatory action	1734:1761	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	6	55	theme	material	1246:1253	arg1	35:1 ml/g					1255:1263	material 35:1 ml/g	1246:1263	material 35:1 ml/g	1246:1263	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	3	56	theme	STUDY	545:549	arg1	AIM					534:536	AIM	534:536	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L.	534:696	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	7	57	theme	phagocytic	1519:1528	arg1	activity					1530:1537	the phagocytic activity	1515:1537	the phagocytic activity	1515:1537	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	6	58	theme	solvent	1235:1241	arg1	concentration					1183:1195	ethanol concentration	1175:1195	ethanol concentration 30%	1175:1199	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	58	theme	solvent	1235:1241	arg1	temperature					1280:1290	extraction temperature 46 °C	1269:1296	extraction temperature 46 °C	1269:1296	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	58	theme	solvent	1235:1241	arg1	55 min					1218:1223	extraction time 55 min	1202:1223	extraction time 55 min	1202:1223	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	58	theme	solvent	1235:1241	arg1	ratio					1226:1230	ratio	1226:1230	ratio of solvent to material 35:1 ml/g	1226:1263	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	6	59	theme	surface	1325:1331	arg1	method					1333:1338	response surface method	1316:1338	response surface method	1316:1338	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	1	60	theme	RELEVANCE	202:210	arg1	L.					232:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	7	61	theme	anti-inflammatory	1478:1494	arg1	effect					1496:1501	a stronger anti-inflammatory effect	1467:1501	a stronger anti-inflammatory effect	1467:1501	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	1	62	theme	terrestris	221:230	arg1	L.					232:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L.	181:233	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	9	63	from	drug	1956:1959	arg1	clinic					1964:1969	clinic	1964:1969	clinic	1964:1969	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	3	64	theme	major	615:619	arg1	components					621:630	major components	615:630	major components	615:630	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	8	65	theme	TLR4-TRAF6-NF-κB	1806:1821	arg1	pathway					1834:1840	the TLR4-TRAF6-NF-κB signalling pathway	1802:1840	the TLR4-TRAF6-NF-κB signalling pathway	1802:1840	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	5	66	theme	anti-inflammatory	960:976	arg1	activity					978:985	The anti-inflammatory activity	956:985	The anti-inflammatory activity of total saponins	956:1003	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	7	67	theme	TNF-α	1663:1667	arg1	content					1645:1651	the content	1641:1651	the content of NO and TNF-α	1641:1667	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	4	68	theme	LC-MSn	941:946	arg1	method					948:953	LC-MSn method	941:953	LC-MSn method	941:953	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	7	69	theme	saponins	1440:1447	arg1	fraction					1449:1456	saponins fraction	1440:1456	saponins fraction	1440:1456	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	4	70	theme	ultrasonic	741:750	arg1	extraction					752:761	The ultrasonic extraction and response surface methods	737:790	extraction	752:761	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	9	71	dep	CONCLUSION	1843:1852	arg1	imply					1868:1872	imply	1868:1872	imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic	1868:1969	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	8	72	theme	pathway	1834:1840	arg1	activation					1788:1797	the activation	1784:1797	the activation of the TLR4-TRAF6-NF-κB signalling pathway	1784:1840	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	7	73	theme	NO	1656:1657	arg1	content					1645:1651	the content	1641:1651	the content of NO and TNF-α	1641:1667	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	4	74	dep	L.	890:891	arg1	leaves					893:898	leaves	893:898	leaves	893:898	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	0	75	theme	anti-inflammatory	118:134	arg1	activity					136:143	anti-inflammatory activity	118:143	anti-inflammatory activity	118:143	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	0	75	theme	anti-inflammatory	118:134	arg1	saponins					71:78	saponins	71:78	saponins	71:78	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	5	76	theme	RAW	1047:1049	arg1	264.7 cells					1051:1061	lipopolysaccharide induced RAW 264.7 cells	1020:1061	lipopolysaccharide induced RAW 264.7 cells	1020:1061	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	77	dep	RESULTS	1098:1104	arg1	parameters					1132:1141	The ultrasonic extraction parameters	1106:1141	RESULTS The ultrasonic extraction parameters	1098:1141	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	2	78	theme	chemical	400:407	arg1	compositions					409:420	chemical compositions	400:420	chemical compositions	400:420	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	5	79	theme	saponins	996:1003	arg1	activity					978:985	The anti-inflammatory activity	956:985	The anti-inflammatory activity of total saponins	956:1003	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	80	theme	saponins	1146:1153	arg1	fraction					1155:1162	saponins fraction	1146:1162	saponins fraction	1146:1162	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	2	81	theme	total	465:469	arg1	saponins					471:478	total saponins	465:478	total saponins from Tribulus terrestris L.	465:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	4	82	theme	surface	776:782	arg1	methods					784:790	The ultrasonic extraction and response surface methods	737:790	methods	784:790	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	0	83	theme	extraction	27:36	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasound extraction of Tribulus terrestris L.	0:62	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	1	84	theme	biological	295:304	arg1	activities					306:315	many biological activities	290:315	many biological activities	290:315	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	3	85	theme	Tribulus	675:682	arg1	L.					695:696	Tribulus terrestris L.	675:696	Tribulus terrestris L.	675:696	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	0	86	theme	Tribulus	41:48	arg1	terrestris					50:59	Tribulus terrestris L.	41:62	Tribulus terrestris L.	41:62	Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo.					
34038799	7	87	theme	tissues	1617:1623	arg1	morphology					1578:1587	morphology	1578:1587	morphology of RAW 264.7 cells and lung tissues	1578:1623	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	5	88	theme	mice	1085:1088	arg1	models					1090:1095	lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models	1020:1095	lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models	1020:1095	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	89	theme	ultrasonic	1110:1119	arg1	parameters					1132:1141	The ultrasonic extraction parameters	1106:1141	RESULTS The ultrasonic extraction parameters	1098:1141	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	2	90	from	process	391:397	arg1	terrestris					494:503	Tribulus terrestris L.	485:506	Tribulus terrestris L.	485:506	However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.					
34038799	6	91	theme	time	1213:1216	arg1	55 min					1218:1223	extraction time 55 min	1202:1223	extraction time 55 min	1202:1223	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	8	92	theme	saponins	1707:1714	arg1	extract					1716:1722	total saponins extract	1701:1722	total saponins extract	1701:1722	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	1	93	theme	chemical	331:338	arg1	saponins					357:364	saponins	357:364	saponins	357:364	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	1	93	theme	chemical	331:338	arg1	constituents					340:351	its main chemical constituents	322:351	its main chemical constituents	322:351	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	8	94	theme	signalling	1823:1832	arg1	pathway					1834:1840	the TLR4-TRAF6-NF-κB signalling pathway	1802:1840	the TLR4-TRAF6-NF-κB signalling pathway	1802:1840	Moreover, it was revealed that total saponins extract can exert the anti-inflammatory action by the inhibition of the activation of the TLR4-TRAF6-NF-κB signalling pathway.					
34038799	7	95	theme	RAW	1592:1594	arg1	264.7 cells					1596:1606	RAW 264.7 cells	1592:1606	RAW 264.7 cells	1592:1606	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	9	96	theme	important	1928:1936	arg1	saponins					1909:1916	saponins	1909:1916	saponins	1909:1916	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	9	96	theme	important	1928:1936	arg1	drug					1956:1959	an important anti-inflammatory drug	1925:1959	an important anti-inflammatory drug in clinic	1925:1969	CONCLUSION These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.					
34038799	5	97	theme	264.7 cells	1051:1061	arg1	models					1090:1095	lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models	1020:1095	lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models	1020:1095	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	3	98	theme	L.	695:696	arg1	process					606:612	the extraction process	591:612	the extraction process	591:612	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	3	98	theme	L.	695:696	arg1	components					621:630	major components	615:630	major components	615:630	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	3	98	theme	L.	695:696	arg1	action					651:656	anti-inflammatory action	633:656	anti-inflammatory action	633:656	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	3	98	theme	L.	695:696	arg1	mechanism					662:670	mechanism	662:670	mechanism	662:670	AIM OF THE STUDY The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.					
34038799	4	99	theme	technology	836:845	arg1	optimization					809:820	optimization	809:820	optimization of extraction technology of total saponins from Tribulus terrestris L. leaves	809:898	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
34038799	1	100	theme	annual	242:247	arg1	plant					254:258	an annual herb plant	239:258	an annual herb plant from Zygophyllaceae	239:278	ETHNOPHARMACOLOGICAL RELEVANCE Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins.					
34038799	7	101	theme	morphology	1578:1587	arg1	activity					1530:1537	the phagocytic activity	1515:1537	the phagocytic activity	1515:1537	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	7	101	theme	morphology	1578:1587	arg1	edema					1553:1557	pulmonary edema	1543:1557	pulmonary edema	1543:1557	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	7	101	theme	morphology	1578:1587	arg1	protection					1564:1573	protection	1564:1573	protection of morphology of RAW 264.7 cells and lung tissues	1564:1623	Moreover, saponins fraction can play a stronger anti-inflammatory effect by reducing the phagocytic activity and pulmonary edema, and protection of morphology of RAW 264.7 cells and lung tissues, and decreasing the content of NO and TNF-α.					
34038799	5	102	theme	lung	1073:1076	arg1	injury					1078:1083	acute lung injury	1067:1083	acute lung injury	1067:1083	The anti-inflammatory activity of total saponins was studied by lipopolysaccharide induced RAW 264.7 cells and acute lung injury mice models.					
34038799	6	103	theme	30	1197:1198	arg1	%					1199:1199	%	1199:1199	%	1199:1199	RESULTS The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MSn method.					
34038799	4	104	theme	total	850:854	arg1	saponins					856:863	total saponins	850:863	total saponins from Tribulus terrestris L. leaves	850:898	MATERIALS AND METHODS The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MSn method.					
33091471	5	0	theme	thermodynamic	778:790	arg1	parameters					792:801	thermodynamic parameters	778:801	thermodynamic parameters	778:801	The estimation of thermodynamic parameters showed that there was an increase in the entropy change, and that the bioadsorption process is endothermic and spontaneous.					
33091471	5	1	theme	entropy	844:850	arg1	change					852:857	the entropy change	840:857	the entropy change	840:857	The estimation of thermodynamic parameters showed that there was an increase in the entropy change, and that the bioadsorption process is endothermic and spontaneous.					
33091471	1	2	theme	present	163:169	arg1	protein					154:160	a soluble globular protein	135:160	a soluble globular protein	135:160	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	1	2	theme	present	163:169	arg1	Sericin					124:130	Sericin	124:130	Sericin	124:130	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	5	3	theme	parameters	792:801	arg1	estimation					764:773	The estimation	760:773	The estimation of thermodynamic parameters	760:801	The estimation of thermodynamic parameters showed that there was an increase in the entropy change, and that the bioadsorption process is endothermic and spontaneous.					
33091471	6	4	theme	Characterization	927:942	arg1	analyzes					944:951	Characterization analyzes	927:951	Characterization analyzes	927:951	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	7	5	theme	first	1148:1152	arg1	cycle					1167:1171	the first regeneration cycle	1144:1171	the first regeneration cycle	1144:1171	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	1	6	from	present	163:169	arg1	cocoons					195:201	Bombyx mori silkworm cocoons	174:201	Bombyx mori silkworm cocoons	174:201	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	4	7	theme	Equilibrium	692:702	arg1	data					704:707	55 °C. Equilibrium data	685:707	55 °C. Equilibrium data	685:707	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	3	8	from	bioadsorption	515:527	arg1	medium					574:579	aqueous medium	566:579	aqueous medium	566:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	6	9	theme	spherical	1027:1035	arg1	shape					1037:1041	spherical shape	1027:1041	spherical shape	1027:1041	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	8	10	theme	synthetic	1379:1387	arg1	effluent					1389:1396	synthetic effluent	1379:1396	synthetic effluent containing different toxic metal ions	1379:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	2	11	theme	other	292:296	arg1	agent					359:363	alginate polysaccharide and crosslinking agent poly(vinyl alcohol)	318:383	agent	359:363	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	2	11	theme	other	292:296	arg1	substances					298:307	other substances	292:307	other substances	292:307	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	8	12	theme	metal	1425:1429	arg1	ions					1431:1434	different toxic metal ions	1409:1434	different toxic metal ions	1409:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	9	13	theme	SAPVA	1465:1469	arg1	bioabsorbent					1509:1520	an effective bioabsorbent	1496:1520	an effective bioabsorbent	1496:1520	These results show that the SAPVA particles can be used as an effective bioabsorbent to remove and recover ytterbium from wastewater.					
33091471	9	13	theme	SAPVA	1465:1469	arg1	particles					1471:1479	the SAPVA particles	1461:1479	the SAPVA particles	1461:1479	These results show that the SAPVA particles can be used as an effective bioabsorbent to remove and recover ytterbium from wastewater.					
33091471	0	14	theme	regeneration	102:113	arg1	studies					115:121	regeneration studies	102:121	regeneration studies	102:121	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	2	15	dep	agent	359:363	arg1	poly					365:368	poly	365:368	poly(vinyl alcohol)	365:383	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	2	15	dep	agent	359:363	arg1	alcohol					376:382	vinyl alcohol	370:382	vinyl alcohol	370:382	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	1	16	theme	globular	145:152	arg1	protein					154:160	a soluble globular protein	135:160	a soluble globular protein	135:160	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	1	16	theme	globular	145:152	arg1	Sericin					124:130	Sericin	124:130	Sericin	124:130	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	7	17	theme	ions	1207:1210	arg1	ions					1207:1210	captured ytterbium ions	1188:1210	captured ytterbium ions	1188:1210	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	7	17	theme	ions	1207:1210	arg1	amount					1178:1183	the amount	1174:1183	the amount of captured ytterbium ions	1174:1210	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	3	18	theme	vinyl	482:486	arg1	poly					477:480	poly	477:480	poly(vinyl alcohol) (SAPVA)	477:503	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	3	18	theme	vinyl	482:486	arg1	alcohol					488:494	vinyl alcohol	482:494	vinyl alcohol	482:494	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	0	19	link	Crosslinked	0:10	arg1	particles					29:37	Crosslinked alginate/sericin particles	0:37	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.	0:122	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	19	link	Crosslinked	0:10	arg1	thermodynamic					84:96	thermodynamic	84:96	thermodynamic	84:96	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	19	link	Crosslinked	0:10	arg1	Equilibrium					71:81	Equilibrium	71:81	Equilibrium	71:81	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	5	20	theme	bioadsorption	873:885	arg1	endothermic					898:908	endothermic	898:908	endothermic	898:908	The estimation of thermodynamic parameters showed that there was an increase in the entropy change, and that the bioadsorption process is endothermic and spontaneous.					
33091471	5	20	theme	bioadsorption	873:885	arg1	process					887:893	the bioadsorption process	869:893	the bioadsorption process	869:893	The estimation of thermodynamic parameters showed that there was an increase in the entropy change, and that the bioadsorption process is endothermic and spontaneous.					
33091471	1	21	theme	Bombyx	174:179	arg1	mori					181:184	Bombyx mori	174:184	Bombyx mori silkworm cocoons	174:201	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	3	22	theme	batch	509:513	arg1	bioadsorption					515:527	batch bioadsorption	509:527	batch bioadsorption of rare-earth element ytterbium from aqueous medium	509:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	8	23	contain	containing	1398:1407	arg1	effluent					1389:1396	synthetic effluent	1379:1396	synthetic effluent containing different toxic metal ions	1379:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	8	23	contain	containing	1398:1407	arg2	ions					1431:1434	different toxic metal ions	1409:1434	different toxic metal ions	1409:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	1	24	theme	mori	181:184	arg1	cocoons					195:201	Bombyx mori silkworm cocoons	174:201	Bombyx mori silkworm cocoons	174:201	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	0	25	theme	alginate/sericin	12:27	arg1	particles					29:37	Crosslinked alginate/sericin particles	0:37	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.	0:122	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	25	theme	alginate/sericin	12:27	arg1	thermodynamic					84:96	thermodynamic	84:96	thermodynamic	84:96	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	25	theme	alginate/sericin	12:27	arg1	Equilibrium					71:81	Equilibrium	71:81	Equilibrium	71:81	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	1	26	attach	present	163:169	arg2	Sericin					124:130	Sericin	124:130	Sericin	124:130	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	1	26	attach	present	163:169	arg1	cocoons					195:201	Bombyx mori silkworm cocoons	174:201	Bombyx mori silkworm cocoons	174:201	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	1	26	attach	present	163:169	arg2	protein					154:160	a soluble globular protein	135:160	a soluble globular protein	135:160	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	3	27	theme	ytterbium	551:559	arg1	bioadsorption					515:527	batch bioadsorption	509:527	batch bioadsorption of rare-earth element ytterbium from aqueous medium	509:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	7	28	theme	captured	1188:1195	arg1	ions					1207:1210	captured ytterbium ions	1188:1210	captured ytterbium ions	1188:1210	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	1	29	theme	silkworm	186:193	arg1	cocoons					195:201	Bombyx mori silkworm cocoons	174:201	Bombyx mori silkworm cocoons	174:201	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	0	30	theme	Crosslinked	0:10	arg1	particles					29:37	Crosslinked alginate/sericin particles	0:37	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.	0:122	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	30	theme	Crosslinked	0:10	arg1	thermodynamic					84:96	thermodynamic	84:96	thermodynamic	84:96	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	30	theme	Crosslinked	0:10	arg1	Equilibrium					71:81	Equilibrium	71:81	Equilibrium	71:81	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	4	31	theme	Langmuir	718:725	arg1	models					752:757	both Langmuir and Dubinin-Radushkevich models	713:757	models	752:757	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	6	32	theme	low	1090:1092	arg1	area					1102:1105	low surface area	1090:1105	low surface area	1090:1105	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	4	33	theme	Dubinin-Radushkevich	731:750	arg1	models					752:757	both Langmuir and Dubinin-Radushkevich models	713:757	models	752:757	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	3	34	theme	alginate	418:425	arg1	use					411:413	the use	407:413	the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium	407:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	4	35	theme	equilibrium	586:596	arg1	study					598:602	The equilibrium study	582:602	The equilibrium study	582:602	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	6	36	theme	ytterbium	995:1003	arg1	bioadsorption					1005:1017	ytterbium bioadsorption	995:1017	ytterbium bioadsorption	995:1017	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	7	37	theme	calcium	1261:1267	arg1	ions					1269:1272	calcium ions	1261:1272	calcium ions	1261:1272	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	3	38	theme	rare-earth	532:541	arg1	ytterbium					551:559	rare-earth element ytterbium	532:559	rare-earth element ytterbium from aqueous medium	532:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	3	39	from	medium	574:579	arg1	bioadsorption					515:527	batch bioadsorption	509:527	batch bioadsorption of rare-earth element ytterbium from aqueous medium	509:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	3	39	from	medium	574:579	arg1	ytterbium					551:559	rare-earth element ytterbium	532:559	rare-earth element ytterbium from aqueous medium	532:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	4	40	theme	maximum	620:626	arg1	capacity					642:649	the maximum bioadsorption capacity	616:649	the maximum bioadsorption capacity for ytterbium	616:663	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	4	40	theme	maximum	620:626	arg1	0.642 mmol/g					669:680	0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models	669:757	0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models	669:757	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	8	41	theme	different	1409:1417	arg1	ions					1431:1434	different toxic metal ions	1409:1434	different toxic metal ions	1409:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	3	42	theme	element	543:549	arg1	ytterbium					551:559	rare-earth element ytterbium	532:559	rare-earth element ytterbium from aqueous medium	532:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	8	43	theme	toxic	1419:1423	arg1	ions					1431:1434	different toxic metal ions	1409:1434	different toxic metal ions	1409:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	4	44	theme	55 °C.	685:690	arg1	data					704:707	55 °C. Equilibrium data	685:707	55 °C. Equilibrium data	685:707	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	7	45	theme	SAPVA	1302:1306	arg1	particles					1308:1316	SAPVA particles	1302:1316	SAPVA particles	1302:1316	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	8	46	dep	ytterbium	1364:1372	arg1	effluent					1389:1396	synthetic effluent	1379:1396	synthetic effluent containing different toxic metal ions	1379:1434	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	6	47	theme	surface	1094:1100	arg1	area					1102:1105	low surface area	1090:1105	low surface area	1090:1105	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	3	48	theme	particles	439:447	arg1	use					411:413	the use	407:413	the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium	407:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	8	49	theme	Bioadsorbent	1319:1330	arg1	particles					1332:1340	Bioadsorbent particles	1319:1340	Bioadsorbent particles	1319:1340	Bioadsorbent particles separated selectively ytterbium from synthetic effluent containing different toxic metal ions.					
33091471	4	50	theme	bioadsorption	628:640	arg1	capacity					642:649	the maximum bioadsorption capacity	616:649	the maximum bioadsorption capacity for ytterbium	616:663	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	4	50	theme	bioadsorption	628:640	arg1	0.642 mmol/g					669:680	0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models	669:757	0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models	669:757	The equilibrium study showed that the maximum bioadsorption capacity for ytterbium was 0.642 mmol/g at 55 °C. Equilibrium data fit both Langmuir and Dubinin-Radushkevich models.					
33091471	7	51	theme	regeneration	1154:1165	arg1	cycle					1167:1171	the first regeneration cycle	1144:1171	the first regeneration cycle	1144:1171	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	6	52	theme	amorphous	1069:1077	arg1	structure					1079:1087	amorphous structure	1069:1087	amorphous structure	1069:1087	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	3	53	theme	aqueous	566:572	arg1	medium					574:579	aqueous medium	566:579	aqueous medium	566:579	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	9	54	used	used	1488:1491	arg2	particles					1471:1479	the SAPVA particles	1461:1479	the SAPVA particles	1461:1479	These results show that the SAPVA particles can be used as an effective bioabsorbent to remove and recover ytterbium from wastewater.					
33091471	9	54	used	used	1488:1491	arg2	bioabsorbent					1509:1520	an effective bioabsorbent	1496:1520	an effective bioabsorbent	1496:1520	These results show that the SAPVA particles can be used as an effective bioabsorbent to remove and recover ytterbium from wastewater.					
33091471	2	55	theme	crosslinking	346:357	arg1	agent					359:363	alginate polysaccharide and crosslinking agent poly(vinyl alcohol)	318:383	agent	359:363	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	0	56	theme	ytterbium	60:68	arg1	particles					29:37	Crosslinked alginate/sericin particles	0:37	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.	0:122	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	56	theme	ytterbium	60:68	arg1	thermodynamic					84:96	thermodynamic	84:96	thermodynamic	84:96	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	56	theme	ytterbium	60:68	arg1	Equilibrium					71:81	Equilibrium	71:81	Equilibrium	71:81	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	6	57	theme	SAPVA	967:971	arg1	particles					973:981	SAPVA particles	967:981	SAPVA particles	967:981	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	7	58	dep	showed	1212:1217	arg1	released					1290:1297	released	1290:1297	showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles	1212:1316	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	9	59	theme	effective	1499:1507	arg1	bioabsorbent					1509:1520	an effective bioabsorbent	1496:1520	an effective bioabsorbent	1496:1520	These results show that the SAPVA particles can be used as an effective bioabsorbent to remove and recover ytterbium from wastewater.					
33091471	9	59	theme	effective	1499:1507	arg1	particles					1471:1479	the SAPVA particles	1461:1479	the SAPVA particles	1461:1479	These results show that the SAPVA particles can be used as an effective bioabsorbent to remove and recover ytterbium from wastewater.					
33091471	1	60	theme	soluble	137:143	arg1	protein					154:160	a soluble globular protein	135:160	a soluble globular protein	135:160	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	1	60	theme	soluble	137:143	arg1	Sericin					124:130	Sericin	124:130	Sericin	124:130	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	0	61	dep	particles	29:37	arg1	thermodynamic					84:96	thermodynamic	84:96	thermodynamic	84:96	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	61	dep	particles	29:37	arg1	studies					115:121	regeneration studies	102:121	regeneration studies	102:121	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	61	dep	particles	29:37	arg1	Equilibrium					71:81	Equilibrium	71:81	Equilibrium	71:81	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	0	61	dep	particles	29:37	arg1	particles					29:37	Crosslinked alginate/sericin particles	0:37	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.	0:122	Crosslinked alginate/sericin particles for bioadsorption of ytterbium: Equilibrium, thermodynamic and regeneration studies.					
33091471	2	62	theme	alginate	318:325	arg1	agent					359:363	alginate polysaccharide and crosslinking agent poly(vinyl alcohol)	318:383	agent	359:363	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	2	63	theme	polysaccharide	327:340	arg1	agent					359:363	alginate polysaccharide and crosslinking agent poly(vinyl alcohol)	318:383	agent	359:363	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	6	64	theme	low	1124:1126	arg1	porosity					1128:1135	low porosity	1124:1135	low porosity	1124:1135	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	1	65	from	cocoons	195:201	arg1	present					163:169	present	163:169	present	163:169	Sericin is a soluble globular protein, present in Bombyx mori silkworm cocoons.					
33091471	2	66	theme	vinyl	370:374	arg1	poly					365:368	poly	365:368	poly(vinyl alcohol)	365:383	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	2	66	theme	vinyl	370:374	arg1	alcohol					376:382	vinyl alcohol	370:382	vinyl alcohol	370:382	Sericin's properties can be improved to expand its application by producing blends with other substances, such as alginate polysaccharide and crosslinking agent poly(vinyl alcohol).					
33091471	3	67	theme	sericin	431:437	arg1	particles					439:447	sericin particles	431:447	sericin particles	431:447	This study evaluates the use of alginate and sericin particles chemically crosslinked with poly(vinyl alcohol) (SAPVA) for batch bioadsorption of rare-earth element ytterbium from aqueous medium.					
33091471	6	68	theme	homogeneous	1044:1054	arg1	composition					1056:1066	homogeneous composition	1044:1066	homogeneous composition	1044:1066	Characterization analyzes revealed that SAPVA particles, even after ytterbium bioadsorption, showed spherical shape, homogeneous composition, amorphous structure, low surface area, macropores, and low porosity.					
33091471	5	69	from	increase	828:835	arg1	change					852:857	the entropy change	840:857	the entropy change	840:857	The estimation of thermodynamic parameters showed that there was an increase in the entropy change, and that the bioadsorption process is endothermic and spontaneous.					
33091471	7	70	theme	ytterbium	1197:1205	arg1	ions					1207:1210	captured ytterbium ions	1188:1210	captured ytterbium ions	1188:1210	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
33091471	7	71	theme	slight	1221:1226	arg1	increase					1228:1235	a slight increase	1219:1235	a slight increase (about 0.01 mmol/g)	1219:1255	After the first regeneration cycle, the amount of captured ytterbium ions showed a slight increase (about 0.01 mmol/g) and calcium ions were completely released by SAPVA particles.					
32071263	1	0	theme	gut	128:130	arg1	microbiota					132:141	the human gut microbiota	118:141	the human gut microbiota	118:141	Dietary fibers can be utilized to shape the human gut microbiota.					
32071263	0	1	theme	Gut	62:64	arg1	Microbiota					66:75	Gut Microbiota	62:75	Gut Microbiota	62:75	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	0	2	from	View	4:7	arg1	Selection					26:34	Dietary Fiber Selection	12:34	Dietary Fiber Selection for Predictable Shifts in Gut Microbiota	12:75	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	6	3	theme	independent	838:848	arg1	shifts					831:836	predictable shifts	819:836	predictable shifts independent of the background microbial composition	819:888	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	3	4	theme	divergent	323:331	arg1	responses					339:347	divergent fiber responses	323:347	divergent fiber responses	323:347	Thus, divergent fiber responses are observed across individuals harboring distinct microbial communities.					
32071263	5	5	theme	narrow	654:659	arg1	bacteria					670:677	bacteria	670:677	bacteria within the gut	670:692	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	5	5	theme	narrow	654:659	arg1	group					661:665	only a narrow group	647:665	only a narrow group of bacteria within the gut	647:692	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	3	6	theme	fiber	333:337	arg1	responses					339:347	divergent fiber responses	323:347	divergent fiber responses	323:347	Thus, divergent fiber responses are observed across individuals harboring distinct microbial communities.					
32071263	6	7	theme	specific	785:792	arg1	microbes					794:801	specific microbes	785:801	specific microbes	785:801	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	2	8	theme	competition	236:246	arg1	outcomes					157:164	the outcomes	153:164	the outcomes from most dietary fibers currently used as prebiotics	153:218	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	2	8	theme	competition	236:246	arg1	result					226:231	a result	224:231	a result of competition between microbes with overlapping abilities to utilize these fibers	224:314	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	2	9	from	fibers	184:189	arg1	outcomes					157:164	the outcomes	153:164	the outcomes from most dietary fibers currently used as prebiotics	153:218	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	2	9	from	fibers	184:189	arg1	result					226:231	a result	224:231	a result of competition between microbes with overlapping abilities to utilize these fibers	224:314	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	6	10	theme	composition	878:888	arg1	independent					838:848	independent	838:848	independent	838:848	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	6	11	theme	microbial	868:876	arg1	composition					878:888	the background microbial composition	853:888	the background microbial composition	853:888	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	5	12	theme	chemical	575:582	arg1	characteristics					597:611	chemical and physical characteristics	575:611	chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate	575:733	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	0	13	theme	New	0:2	arg1	View					4:7	New View	0:7	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.	0:76	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	1	14	theme	Dietary	78:84	arg1	fibers					86:91	Dietary fibers	78:91	Dietary fibers	78:91	Dietary fibers can be utilized to shape the human gut microbiota.					
32071263	0	15	theme	Dietary	12:18	arg1	Selection					26:34	Dietary Fiber Selection	12:34	Dietary Fiber Selection for Predictable Shifts in Gut Microbiota	12:75	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	5	16	theme	physical	588:595	arg1	characteristics					597:611	chemical and physical characteristics	575:611	chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate	575:733	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	2	17	theme	overlapping	270:280	arg1	abilities					282:290	overlapping abilities to utilize these fibers	270:314	overlapping abilities to utilize these fibers	270:314	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	6	18	theme	background	857:866	arg1	composition					878:888	the background microbial composition	853:888	the background microbial composition	853:888	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	3	19	theme	distinct	391:398	arg1	communities					410:420	distinct microbial communities	391:420	distinct microbial communities	391:420	Thus, divergent fiber responses are observed across individuals harboring distinct microbial communities.					
32071263	0	20	theme	Fiber	20:24	arg1	Selection					26:34	Dietary Fiber Selection	12:34	Dietary Fiber Selection for Predictable Shifts in Gut Microbiota	12:75	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	3	21	theme	microbial	400:408	arg1	communities					410:420	distinct microbial communities	391:420	distinct microbial communities	391:420	Thus, divergent fiber responses are observed across individuals harboring distinct microbial communities.					
32071263	4	22	theme	dietary	445:451	arg1	fibers					453:458	dietary fibers	445:458	dietary fibers	445:458	Here, we propose that dietary fibers can be classified hierarchically according to their specificity toward gut microbes.					
32071263	6	23	theme	such	747:750	arg1	fibers					752:757	such fibers	747:757	such fibers	747:757	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	0	24	theme	Predictable	40:50	arg1	Shifts					52:57	Predictable Shifts	40:57	Predictable Shifts in Gut Microbiota	40:75	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	0	25	from	Shifts	52:57	arg1	Microbiota					66:75	Gut Microbiota	62:75	Gut Microbiota	62:75	New View on Dietary Fiber Selection for Predictable Shifts in Gut Microbiota.					
32071263	6	26	theme	fibers	752:757	arg1	use					740:742	The use	736:742	The use of such fibers as prebiotics targeted to specific microbes	736:801	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	5	27	theme	bacteria	670:677	arg1	bacteria					670:677	bacteria	670:677	bacteria within the gut	670:692	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	5	27	theme	bacteria	670:677	arg1	group					661:665	only a narrow group	647:665	only a narrow group of bacteria within the gut	647:692	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	2	28	theme	dietary	176:182	arg1	fibers					184:189	most dietary fibers	171:189	most dietary fibers currently used as prebiotics	171:218	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	6	29	theme	predictable	819:829	arg1	shifts					831:836	predictable shifts	819:836	predictable shifts independent of the background microbial composition	819:888	The use of such fibers as prebiotics targeted to specific microbes would result in predictable shifts independent of the background microbial composition.					
32071263	5	30	theme	specific	552:559	arg1	fibers					561:566	Highly specific fibers	545:566	Highly specific fibers	545:566	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	4	31	theme	gut	531:533	arg1	microbes					535:542	gut microbes	531:542	gut microbes	531:542	Here, we propose that dietary fibers can be classified hierarchically according to their specificity toward gut microbes.					
32071263	2	32	theme	most	171:174	arg1	fibers					184:189	most dietary fibers	171:189	most dietary fibers currently used as prebiotics	171:218	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	1	33	used	utilized	100:107	arg2	fibers					86:91	Dietary fibers	78:91	Dietary fibers	78:91	Dietary fibers can be utilized to shape the human gut microbiota.					
32071263	2	34	with	competition	236:246	arg1	abilities					282:290	overlapping abilities to utilize these fibers	270:314	overlapping abilities to utilize these fibers	270:314	However, the outcomes from most dietary fibers currently used as prebiotics are a result of competition between microbes with overlapping abilities to utilize these fibers.					
32071263	5	35	used	utilized	635:642	arg2	them					624:627	them	624:627	them	624:627	Highly specific fibers harbor chemical and physical characteristics that allow them to be utilized by only a narrow group of bacteria within the gut, reducing competition for that substrate.					
32071263	1	36	theme	human	122:126	arg1	microbiota					132:141	the human gut microbiota	118:141	the human gut microbiota	118:141	Dietary fibers can be utilized to shape the human gut microbiota.					
32446893	5	0	from	vein	934:937	arg1	models					955:960	rat models	951:960	rat models	951:960	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	8	1	from	reintroduction	1308:1321	arg1	fields					1344:1349	hemostatic fields	1333:1349	hemostatic fields	1333:1349	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	5	2	with	sponge	803:808	arg1	effects					830:836	low exothermic effects	815:836	low exothermic effects	815:836	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	0	3	with	sponge	58:63	arg1	healing					97:103	wound healing	91:103	wound healing	91:103	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	3	4	theme	antibacterial	446:458	arg1	performances					479:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	4	5	theme	whole	685:689	arg1	system					703:708	whole coagulation system	685:708	whole coagulation system activation	685:719	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	1	6	from	hemostats	158:166	arg1	silicates					179:187	natural silicates	171:187	natural silicates	171:187	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	8	7	theme	safe-effective	1358:1371	arg1	avenue					1373:1378	a safe-effective avenue	1356:1378	a safe-effective avenue for clays to control bleeding	1356:1408	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	7	8	theme	cytotoxic	1164:1172	arg1	hemostat					1233:1240	a little hemolytic and skin irritant hemostat	1196:1240	a little hemolytic and skin irritant hemostat	1196:1240	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	7	8	theme	cytotoxic	1164:1172	arg1	sponge					1174:1179	the low cytotoxic sponge	1156:1179	the low cytotoxic sponge	1156:1179	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	5	9	theme	blood	897:901	arg1	loss					903:906	nearly 95% blood loss	886:906	nearly 95% blood loss	886:906	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	5	10	theme	PM	800:801	arg1	sponge					803:808	PM sponge	800:808	PM sponge with low exothermic effects	800:836	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	3	11	theme	hemorrhage	517:526	arg1	control					528:534	massive hemorrhage control	509:534	massive hemorrhage control	509:534	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	6	12	theme	antibacterial	1050:1062	arg1	activity					1064:1071	antibacterial activity	1050:1071	antibacterial activity to Escherichia coli	1050:1091	Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing.					
32446893	6	13	theme	adhesion	1002:1009	arg1	~47 kPa					1012:1018	the intensive tissue adhesion (~47 kPa)	981:1019	the intensive tissue adhesion (~47 kPa)	981:1019	Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing.					
32446893	3	14	theme	adhesion	436:443	arg1	performances					479:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	4	15	theme	cells	653:657	arg1	aggregation					659:669	cells aggregation	653:669	cells aggregation	653:669	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	3	16	theme	healing	471:477	arg1	performances					479:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	1	17	theme	natural	171:177	arg1	silicates					179:187	natural silicates	171:187	natural silicates	171:187	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	0	18	theme	rapid	69:73	arg1	hemostasis					75:84	rapid hemostasis	69:84	rapid hemostasis	69:84	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	8	19	theme	MMT	1326:1328	arg1	reintroduction					1308:1321	reintroduction	1308:1321	reintroduction of MMT in hemostatic fields	1308:1349	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	6	20	theme	PM	1022:1023	arg1	sponge					1025:1030	PM sponge	1022:1030	PM sponge	1022:1030	Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing.					
32446893	0	21	theme	Efficient	0:8	arg1	sponge					58:63	Efficient antibacterial dextran-montmorillonite composite sponge	0:63	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.	0:104	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	4	22	theme	MMT	594:596	arg1	synergism					573:581	perfect synergism	565:581	perfect synergism of PDA and MMT	565:596	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	0	23	theme	antibacterial	10:22	arg1	sponge					58:63	Efficient antibacterial dextran-montmorillonite composite sponge	0:63	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.	0:104	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	3	24	theme	wound	465:469	arg1	healing					471:477	wound healing	465:477	wound healing	465:477	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	6	25	theme	wound	1128:1132	arg1	healing					1134:1140	wound healing	1128:1140	wound healing	1128:1140	Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing.					
32446893	1	26	theme	Montmorillonite	106:120	arg1	powder					128:133	Montmorillonite (MMT) powder	106:133	Montmorillonite (MMT) powder	106:133	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	1	26	theme	Montmorillonite	106:120	arg1	hemostats					158:166	as the most effective hemostats	136:166	as the most effective hemostats in natural silicates	136:187	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	4	27	theme	PM	603:604	arg1	sponge					606:611	the PM sponge	599:611	the PM sponge	599:611	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	3	28	theme	poly	358:361	arg1	PM					408:409	PM	408:409	PM	408:409	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	28	theme	poly	358:361	arg1	sponge					400:405	poly aldehyde dextran (PDA)/MMT composite sponge	358:405	poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances	358:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	5	29	dep	artery	923:928	arg1	incision					939:946	incision	939:946	incision	939:946	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	5	29	dep	artery	923:928	arg1	the					911:913	the	911:913	the	911:913	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	5	30	from	artery	923:928	arg1	models					955:960	rat models	951:960	rat models	951:960	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	3	31	theme	/MMT	385:388	arg1	PM					408:409	PM	408:409	PM	408:409	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	31	theme	/MMT	385:388	arg1	sponge					400:405	poly aldehyde dextran (PDA)/MMT composite sponge	358:405	poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances	358:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	0	32	theme	composite	48:56	arg1	sponge					58:63	Efficient antibacterial dextran-montmorillonite composite sponge	0:63	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.	0:104	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	1	33	theme	MMT	123:125	arg1	powder					128:133	Montmorillonite (MMT) powder	106:133	Montmorillonite (MMT) powder	106:133	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	1	33	theme	MMT	123:125	arg1	hemostats					158:166	as the most effective hemostats	136:166	as the most effective hemostats in natural silicates	136:187	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	5	34	dep	loss	903:906	arg1	%					895:895	%	895:895	%	895:895	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	0	35	theme	dextran-montmorillonite	24:46	arg1	sponge					58:63	Efficient antibacterial dextran-montmorillonite composite sponge	0:63	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.	0:104	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	4	36	theme	coagulation	691:701	arg1	system					703:708	whole coagulation system	685:708	whole coagulation system activation	685:719	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	3	37	theme	massive	509:515	arg1	control					528:534	massive hemorrhage control	509:534	massive hemorrhage control	509:534	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	4	38	theme	synergism	573:581	arg1	degree					555:560	the high degree	546:560	the high degree of perfect synergism of PDA and MMT	546:596	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	7	39	theme	low	1160:1162	arg1	hemostat					1233:1240	a little hemolytic and skin irritant hemostat	1196:1240	a little hemolytic and skin irritant hemostat	1196:1240	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	7	39	theme	low	1160:1162	arg1	sponge					1174:1179	the low cytotoxic sponge	1156:1179	the low cytotoxic sponge	1156:1179	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	5	40	dep	%	895:895	arg1	95					893:894	95	893:894	95	893:894	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	8	41	theme	PM	1267:1268	arg1	sponge					1270:1275	the biocompatible PM sponge	1249:1275	the biocompatible PM sponge	1249:1275	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	1	42	theme	commercial	208:217	arg1	application					219:229	commercial application	208:229	commercial application due to its embolic effect	208:255	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	3	43	theme	composite	390:398	arg1	PM					408:409	PM	408:409	PM	408:409	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	43	theme	composite	390:398	arg1	sponge					400:405	poly aldehyde dextran (PDA)/MMT composite sponge	358:405	poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances	358:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	44	theme	PDA	381:383	arg1	PM					408:409	PM	408:409	PM	408:409	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	44	theme	PDA	381:383	arg1	sponge					400:405	poly aldehyde dextran (PDA)/MMT composite sponge	358:405	poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances	358:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	7	45	theme	little	1198:1203	arg1	hemostat					1233:1240	a little hemolytic and skin irritant hemostat	1196:1240	a little hemolytic and skin irritant hemostat	1196:1240	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	7	45	theme	little	1198:1203	arg1	sponge					1174:1179	the low cytotoxic sponge	1156:1179	the low cytotoxic sponge	1156:1179	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	5	46	theme	femoral	915:921	arg1	artery					923:928	femoral artery	915:928	femoral artery	915:928	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	4	47	theme	PDA	586:588	arg1	synergism					573:581	perfect synergism	565:581	perfect synergism of PDA and MMT	565:596	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	3	48	theme	aldehyde	363:370	arg1	PM					408:409	PM	408:409	PM	408:409	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	48	theme	aldehyde	363:370	arg1	sponge					400:405	poly aldehyde dextran (PDA)/MMT composite sponge	358:405	poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances	358:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	6	49	theme	intensive	985:993	arg1	~47 kPa					1012:1018	the intensive tissue adhesion (~47 kPa)	981:1019	the intensive tissue adhesion (~47 kPa)	981:1019	Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing.					
32446893	2	50	theme	MMT	318:320	arg1	leakage					307:313	the leakage	303:313	the leakage of MMT	303:320	Until now, it's still a challenge to control the leakage of MMT and avoid its side-effects.					
32446893	3	51	theme	dextran	372:378	arg1	PM					408:409	PM	408:409	PM	408:409	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	3	51	theme	dextran	372:378	arg1	sponge					400:405	poly aldehyde dextran (PDA)/MMT composite sponge	358:405	poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances	358:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	8	52	theme	hemostatic	1333:1342	arg1	fields					1344:1349	hemostatic fields	1333:1349	hemostatic fields	1333:1349	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	4	53	theme	clotting	745:752	arg1	time					754:757	clotting time	745:757	clotting time	745:757	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	7	54	theme	hemolytic	1205:1213	arg1	hemostat					1233:1240	a little hemolytic and skin irritant hemostat	1196:1240	a little hemolytic and skin irritant hemostat	1196:1240	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	7	54	theme	hemolytic	1205:1213	arg1	sponge					1174:1179	the low cytotoxic sponge	1156:1179	the low cytotoxic sponge	1156:1179	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	5	55	theme	exothermic	819:828	arg1	effects					830:836	low exothermic effects	815:836	low exothermic effects	815:836	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	7	56	theme	irritant	1224:1231	arg1	hemostat					1233:1240	a little hemolytic and skin irritant hemostat	1196:1240	a little hemolytic and skin irritant hemostat	1196:1240	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	7	56	theme	irritant	1224:1231	arg1	sponge					1174:1179	the low cytotoxic sponge	1156:1179	the low cytotoxic sponge	1156:1179	Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat.					
32446893	8	57	theme	biocompatible	1253:1265	arg1	sponge					1270:1275	the biocompatible PM sponge	1249:1275	the biocompatible PM sponge	1249:1275	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	3	58	theme	commendable	417:427	arg1	adhesion					436:443	commendable tissue adhesion	417:443	commendable tissue adhesion	417:443	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	5	59	theme	limited	861:867	arg1	time					869:872	limited time	861:872	limited time	861:872	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	3	60	with	sponge	400:405	arg1	performances					479:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	commendable tissue adhesion, antibacterial, and wound healing performances	417:490	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	5	61	theme	low	815:817	arg1	effects					830:836	low exothermic effects	815:836	low exothermic effects	815:836	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	4	62	theme	system	703:708	arg1	activation					710:719	whole coagulation system activation	685:719	whole coagulation system activation	685:719	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	3	63	theme	tissue	429:434	arg1	adhesion					436:443	commendable tissue adhesion	417:443	commendable tissue adhesion	417:443	Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control.					
32446893	5	64	from	hemostasis	847:856	arg1	time					869:872	limited time	861:872	limited time	861:872	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	4	65	theme	high	550:553	arg1	degree					555:560	the high degree	546:560	the high degree of perfect synergism of PDA and MMT	546:596	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	0	66	theme	wound	91:95	arg1	healing					97:103	wound healing	91:103	wound healing	91:103	Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.					
32446893	5	67	theme	rat	951:953	arg1	models					955:960	rat models	951:960	rat models	951:960	Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models.					
32446893	6	68	theme	tissue	995:1000	arg1	~47 kPa					1012:1018	the intensive tissue adhesion (~47 kPa)	981:1019	the intensive tissue adhesion (~47 kPa)	981:1019	Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing.					
32446893	4	69	dep	<10 s	773:777	arg1	to					770:771	to	770:771	to	770:771	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	4	70	theme	perfect	565:571	arg1	synergism					573:581	perfect synergism	565:581	perfect synergism of PDA and MMT	565:596	Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro.					
32446893	8	71	theme	new	1291:1293	arg1	strategy					1295:1302	a new strategy	1289:1302	a new strategy for reintroduction of MMT in hemostatic fields	1289:1349	Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.					
32446893	1	72	theme	effective	148:156	arg1	powder					128:133	Montmorillonite (MMT) powder	106:133	Montmorillonite (MMT) powder	106:133	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	1	72	theme	effective	148:156	arg1	hemostats					158:166	as the most effective hemostats	136:166	as the most effective hemostats in natural silicates	136:187	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
32446893	1	73	theme	embolic	242:248	arg1	effect					250:255	its embolic effect	238:255	its embolic effect	238:255	Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect.					
34509956	0	0	theme	emulsions	89:97	arg1	stability					42:50	the emulsification and oxidative stability	9:50	the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	9:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	2	1	used	used	380:383	arg2	pectin					362:367	pectin	362:367	pectin (PEC)	362:373	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	2	1	used	used	380:383	arg2	OVA					353:355	OVA	353:355	OVA	353:355	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	2	1	used	used	380:383	arg2	ovalbumin					342:350	small and uniform (su) ovalbumin	319:350	small and uniform (su) ovalbumin (OVA)	319:356	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	2	1	used	used	380:383	arg2	PEC					370:372	PEC	370:372	PEC	370:372	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	4	2	theme	interfacial	744:754	arg1	properties					799:808	The interfacial, rheological, emulsifying, and antioxidant properties	740:808	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions	740:839	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	2	theme	interfacial	744:754	arg1	excellent					846:854	excellent	846:854	excellent	846:854	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	2	3	theme	small	319:323	arg1	OVA					353:355	OVA	353:355	OVA	353:355	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	2	3	theme	small	319:323	arg1	ovalbumin					342:350	small and uniform (su) ovalbumin	319:350	small and uniform (su) ovalbumin (OVA)	319:356	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	6	4	theme	PSO	1187:1189	arg1	applications					1163:1174	the food-related applications	1146:1174	the food-related applications of OVA and PSO	1146:1189	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	0	5	theme	oil	85:87	arg1	emulsions					89:97	ovalbumin-pectin-pumpkin seed oil emulsions	55:97	ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	55:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	5	6	theme	freeze-thaw	1004:1014	arg1	cycle					1016:1020	a freeze-thaw cycle	1002:1020	a freeze-thaw cycle	1002:1020	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	6	7	theme	food-related	1150:1161	arg1	applications					1163:1174	the food-related applications	1146:1174	the food-related applications of OVA and PSO	1146:1189	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	0	8	theme	ovalbumin	105:113	arg1	solution					115:122	ovalbumin solution	105:122	ovalbumin solution prepared by ultrasound	105:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	0	9	from	Study	0:4	arg1	stability					42:50	the emulsification and oxidative stability	9:50	the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	9:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	4	10	theme	rheological	757:767	arg1	properties					799:808	The interfacial, rheological, emulsifying, and antioxidant properties	740:808	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions	740:839	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	10	theme	rheological	757:767	arg1	excellent					846:854	excellent	846:854	excellent	846:854	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	3	11	theme	interfacial	695:705	arg1	film					707:710	a more stable interfacial film	681:710	a more stable interfacial film on the surface of droplets	681:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	2	12	theme	emulsion	420:427	arg1	form					409:412	the form	405:412	the form of an emulsion	405:427	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	5	13	theme	emulsions	976:984	arg1	stability					945:953	the physical stability	932:953	the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment	932:1040	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	3	14	from	formation	668:676	arg1	surface					719:725	the surface	715:725	the surface of droplets	715:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	3	15	theme	film	707:710	arg1	formation					668:676	the formation	664:676	the formation of a more stable interfacial film on the surface of droplets	664:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	2	16	dep	uniform	329:335	arg1	su					338:339	su	338:339	su	338:339	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	4	17	theme	antioxidant	787:797	arg1	properties					799:808	The interfacial, rheological, emulsifying, and antioxidant properties	740:808	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions	740:839	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	17	theme	antioxidant	787:797	arg1	excellent					846:854	excellent	846:854	excellent	846:854	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	5	18	theme	linolenic	1082:1090	arg1	acid					1092:1095	linolenic acid	1082:1095	linolenic acid	1082:1095	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	5	19	theme	salt	989:992	arg1	stress					994:999	salt stress	989:999	salt stress	989:999	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	3	20	theme	stable	688:693	arg1	film					707:710	a more stable interfacial film	681:710	a more stable interfacial film on the surface of droplets	681:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	3	21	with	solution	572:579	arg1	size					597:600	a droplet size	587:600	a droplet size of 230.13 ± 14.10 nm	587:621	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	4	22	theme	suOVA	906:910	arg1	solution					912:919	PEC and suOVA solution	898:919	PEC and suOVA solution	898:919	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	3	23	theme	230.13 ± 14.10 nm	605:621	arg1	size					597:600	a droplet size	587:600	a droplet size of 230.13 ± 14.10 nm	587:621	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	6	24	theme	self-assembly	1258:1270	arg1	exploration					1239:1249	further exploration	1231:1249	further exploration of the self-assembly of composite emulsions by small and uniform proteins	1231:1323	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	3	25	from	surface	719:725	arg1	formation					668:676	the formation	664:676	the formation of a more stable interfacial film on the surface of droplets	664:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	6	26	theme	composite	1275:1283	arg1	emulsions					1285:1293	composite emulsions	1275:1293	composite emulsions	1275:1293	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	3	27	with	emulsion	468:475	arg1	size					492:495	a droplet size	482:495	a droplet size of 9.82 ± 0.05 μm	482:513	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	1	28	theme	various	256:262	arg1	diseases					272:279	various chronic diseases	256:279	various chronic diseases	256:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	5	29	theme	physical	936:943	arg1	stability					945:953	the physical stability	932:953	the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment	932:1040	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	0	30	theme	emulsification	13:26	arg1	stability					42:50	the emulsification and oxidative stability	9:50	the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	9:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	1	31	theme	chronic	264:270	arg1	diseases					272:279	various chronic diseases	256:279	various chronic diseases	256:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	32	with	compound	192:199	arg1	value					223:227	high nutritional value	206:227	high nutritional value used for the prevention of various chronic diseases	206:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	3	33	theme	suOVA-PEC-PSO	454:466	arg1	emulsion					468:475	suOVA-PEC-PSO emulsion	454:475	suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm	454:513	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	6	34	theme	small	1298:1302	arg1	proteins					1316:1323	small and uniform proteins	1298:1323	small and uniform proteins	1298:1323	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	1	35	theme	diseases	272:279	arg1	prevention					242:251	the prevention	238:251	the prevention of various chronic diseases	238:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	3	36	from	film	707:710	arg1	surface					719:725	the surface	715:725	the surface of droplets	715:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	1	37	theme	valuable	183:190	arg1	compound					192:199	a valuable compound	181:199	a valuable compound with high nutritional value used for the prevention of various chronic diseases	181:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	37	theme	valuable	183:190	arg1	PSO					166:168	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	0	38	theme	oxidative	32:40	arg1	stability					42:50	the emulsification and oxidative stability	9:50	the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	9:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	3	39	theme	300 W	637:641	arg1	ultrasound					643:652	300 W ultrasound	637:652	300 W ultrasound	637:652	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	5	40	theme	acid	1092:1095	arg1	oxidation					1069:1077	the oxidation	1065:1077	the oxidation of linolenic acid	1065:1095	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	4	41	theme	synergistic	870:880	arg1	effects					882:888	the synergistic effects	866:888	the synergistic effects between PEC and suOVA solution	866:919	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	6	42	theme	emulsions	1285:1293	arg1	self-assembly					1258:1270	the self-assembly	1254:1270	the self-assembly of composite emulsions	1254:1293	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	3	43	theme	9.82 ± 0.05 μm	500:513	arg1	size					492:495	a droplet size	482:495	a droplet size of 9.82 ± 0.05 μm	482:513	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	6	44	theme	promising	1206:1214	arg1	foundation					1216:1225	a promising foundation	1204:1225	a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins	1204:1323	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	4	45	theme	PEC	898:900	arg1	solution					912:919	PEC and suOVA solution	898:919	PEC and suOVA solution	898:919	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	46	theme	emulsions	831:839	arg1	properties					799:808	The interfacial, rheological, emulsifying, and antioxidant properties	740:808	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions	740:839	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	46	theme	emulsions	831:839	arg1	excellent					846:854	excellent	846:854	excellent	846:854	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	47	theme	emulsifying	770:780	arg1	properties					799:808	The interfacial, rheological, emulsifying, and antioxidant properties	740:808	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions	740:839	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	4	47	theme	emulsifying	770:780	arg1	excellent					846:854	excellent	846:854	excellent	846:854	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
34509956	5	48	theme	suOVA-PEC-PSO	962:974	arg1	emulsions					976:984	the suOVA-PEC-PSO emulsions	958:984	the suOVA-PEC-PSO emulsions	958:984	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	3	49	theme	droplet	484:490	arg1	size					492:495	a droplet size	482:495	a droplet size of 9.82 ± 0.05 μm	482:513	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	6	50	theme	uniform	1308:1314	arg1	proteins					1316:1323	small and uniform proteins	1298:1323	small and uniform proteins	1298:1323	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	5	51	theme	heat	1027:1030	arg1	treatment					1032:1040	heat treatment	1027:1040	heat treatment	1027:1040	Moreover, the physical stability of the suOVA-PEC-PSO emulsions to salt stress, a freeze-thaw cycle, and heat treatment was also increased and the oxidation of linolenic acid was notably delayed.					
34509956	6	52	theme	further	1231:1237	arg1	exploration					1239:1249	further exploration	1231:1249	further exploration of the self-assembly of composite emulsions by small and uniform proteins	1231:1323	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	3	53	theme	droplets	730:737	arg1	surface					719:725	the surface	715:725	the surface of droplets	715:737	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	6	54	theme	OVA	1179:1181	arg1	applications					1163:1174	the food-related applications	1146:1174	the food-related applications of OVA and PSO	1146:1189	These results have extended the food-related applications of OVA and PSO, and provide a promising foundation for further exploration of the self-assembly of composite emulsions by small and uniform proteins.					
34509956	1	55	theme	seed	156:159	arg1	compound					192:199	a valuable compound	181:199	a valuable compound with high nutritional value used for the prevention of various chronic diseases	181:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	55	theme	seed	156:159	arg1	PSO					166:168	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	56	theme	high	206:209	arg1	value					223:227	high nutritional value	206:227	high nutritional value used for the prevention of various chronic diseases	206:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	57	theme	Pumpkin	148:154	arg1	compound					192:199	a valuable compound	181:199	a valuable compound with high nutritional value used for the prevention of various chronic diseases	181:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	57	theme	Pumpkin	148:154	arg1	PSO					166:168	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	58	theme	nutritional	211:221	arg1	value					223:227	high nutritional value	206:227	high nutritional value used for the prevention of various chronic diseases	206:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	0	59	theme	seed	80:83	arg1	emulsions					89:97	ovalbumin-pectin-pumpkin seed oil emulsions	55:97	ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	55:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	3	60	theme	suOVA	566:570	arg1	solution					572:579	suOVA solution	566:579	suOVA solution (with a droplet size of 230.13 ± 14.10 nm)	566:622	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	2	61	theme	uniform	329:335	arg1	OVA					353:355	OVA	353:355	OVA	353:355	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	2	61	theme	uniform	329:335	arg1	ovalbumin					342:350	small and uniform (su) ovalbumin	319:350	small and uniform (su) ovalbumin (OVA)	319:356	In this work, small and uniform (su) ovalbumin (OVA) and pectin (PEC) were used to stabilize PSO in the form of an emulsion.					
34509956	0	62	theme	ovalbumin-pectin-pumpkin	55:78	arg1	emulsions					89:97	ovalbumin-pectin-pumpkin seed oil emulsions	55:97	ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound	55:145	Study on the emulsification and oxidative stability of ovalbumin-pectin-pumpkin seed oil emulsions using ovalbumin solution prepared by ultrasound.					
34509956	3	63	theme	droplet	589:595	arg1	size					597:600	a droplet size	587:600	a droplet size of 230.13 ± 14.10 nm	587:621	The results showed that suOVA-PEC-PSO emulsion with a droplet size of 9.82 ± 0.05 μm was successfully self-assembled from PSO, PEC, and suOVA solution (with a droplet size of 230.13 ± 14.10 nm) treated with 300 W ultrasound, owing to the formation of a more stable interfacial film on the surface of droplets.					
34509956	1	64	theme	oil	161:163	arg1	compound					192:199	a valuable compound	181:199	a valuable compound with high nutritional value used for the prevention of various chronic diseases	181:279	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	1	64	theme	oil	161:163	arg1	PSO					166:168	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO)	148:169	Pumpkin seed oil (PSO), which is a valuable compound with high nutritional value used for the prevention of various chronic diseases, is prone to oxidation.					
34509956	4	65	theme	suOVA-PES-PSO	817:829	arg1	emulsions					831:839	the suOVA-PES-PSO emulsions	813:839	the suOVA-PES-PSO emulsions	813:839	The interfacial, rheological, emulsifying, and antioxidant properties of the suOVA-PES-PSO emulsions were excellent, owing to the synergistic effects between PEC and suOVA solution.					
32057859	6	0	dep	%	1054:1054	arg1	to					1047:1048	to	1047:1048	to	1047:1048	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	7	1	theme	radical	1139:1145	arg1	ability					1158:1164	ABTS radical scavenging ability	1134:1164	ABTS radical scavenging ability	1134:1164	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	9	2	theme	antioxidant	1373:1383	arg1	activity					1403:1410	good antioxidant and antimicrobial activity	1368:1410	good antioxidant and antimicrobial activity	1368:1410	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	1	3	theme	present	156:162	arg1	study					164:168	the present study	152:168	the present study	152:168	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	4	theme	Chinese	239:245	arg1	CRE					267:269	CRE	267:269	CRE	267:269	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	4	theme	Chinese	239:245	arg1	extract					258:264	Chinese chive root extract	239:264	Chinese chive root extract (CRE)	239:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	0	5	theme	root	100:103	arg1	extract					105:111	Chinese chive (Allium tuberosum) root extract	67:111	Chinese chive (Allium tuberosum) root extract	67:111	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	3	6	theme	potential	602:610	arg1	interaction					612:622	potential interaction	602:622	potential interaction	602:622	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	7	7	dep	57.38	1230:1234	arg1	to					1227:1228	to	1227:1228	to	1227:1228	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	5	8	dep	resulted	809:816	arg1	whereas					885:891	whereas	885:891	whereas	885:891	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	1	9	theme	chive	247:251	arg1	CRE					267:269	CRE	267:269	CRE	267:269	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	9	theme	chive	247:251	arg1	extract					258:264	Chinese chive root extract	239:264	Chinese chive root extract (CRE)	239:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	8	10	theme	CS-CRE5	1252:1258	arg1	film					1260:1263	CS-CRE5 film	1252:1263	CS-CRE5 film	1252:1263	CS-CRE5 film showed the highest biodegradability of 47.36%.					
32057859	9	11	theme	packaging	1472:1480	arg1	material					1482:1489	bio-composite food packaging material	1453:1489	bio-composite food packaging material	1453:1489	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	9	11	theme	packaging	1472:1480	arg1	it					1428:1429	it	1428:1429	it	1428:1429	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	7	12	theme	scavenging	1147:1156	arg1	ability					1158:1164	ABTS radical scavenging ability	1134:1164	ABTS radical scavenging ability	1134:1164	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	2	13	dep	concentrations	320:333	arg1	%					346:346	1, 3 and 5%	336:346	1, 3 and 5%	336:346	CRE at different concentrations (1, 3 and 5% in w/w) were incorporated into the film-forming solution.					
32057859	1	14	theme	root	253:256	arg1	CRE					267:269	CRE	267:269	CRE	267:269	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	14	theme	root	253:256	arg1	extract					258:264	Chinese chive root extract	239:264	Chinese chive root extract (CRE)	239:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	3	15	theme	prepared	649:656	arg1	films					658:662	prepared films	649:662	prepared films	649:662	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	6	16	theme	vapor	989:993	arg1	permeability					995:1006	water vapor permeability	983:1006	water vapor permeability	983:1006	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	1	17	theme	study	164:168	arg1	objective					139:147	The objective	135:147	The objective of the present study	135:168	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	3	18	dep	structure	591:599	arg1	the					587:589	the	587:589	the	587:589	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	19	theme	thermal	628:634	arg1	stability					636:644	thermal stability	628:644	thermal stability	628:644	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	9	20	theme	antimicrobial	1389:1401	arg1	activity					1403:1410	good antioxidant and antimicrobial activity	1368:1410	good antioxidant and antimicrobial activity	1368:1410	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	4	21	theme	higher	693:698	arg1	concentration					708:720	higher extract concentration	693:720	higher extract concentration	693:720	It was revealed by SEM that higher extract concentration triggered the formation of agglomerates within the films.					
32057859	0	22	theme	biodegradable	15:27	arg1	film					51:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	2	23	from	concentrations	320:333	arg1	CRE					303:305	CRE	303:305	CRE at different concentrations (1, 3 and 5% in w/w)	303:354	CRE at different concentrations (1, 3 and 5% in w/w) were incorporated into the film-forming solution.					
32057859	5	24	theme	films	856:860	arg1	properties					838:847	tensile properties	830:847	decrease tensile properties of the films from 28.9 to 15.4 MPa	821:882	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	5	25	theme	CRE	797:799	arg1	Incorporation					780:792	Incorporation	780:792	Incorporation of CRE into CS	780:807	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	0	26	theme	Chitosan-based	0:13	arg1	film					51:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	0	27	dep	Allium	82:87	arg1	tuberosum					89:97	Allium tuberosum	82:97	Allium tuberosum	82:97	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	9	28	theme	bio-composite	1453:1465	arg1	material					1482:1489	bio-composite food packaging material	1453:1489	bio-composite food packaging material	1453:1489	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	9	28	theme	bio-composite	1453:1465	arg1	it					1428:1429	it	1428:1429	it	1428:1429	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	5	29	dep	0.113 mm	931:938	arg1	to					928:929	to	928:929	to	928:929	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	0	30	theme	food	36:39	arg1	film					51:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	5	31	from	properties	838:847	arg1	28.9					867:870	28.9	867:870	28.9	867:870	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	3	32	theme	X-ray	491:495	arg1	diffraction					497:507	X-ray diffraction	491:507	X-ray diffraction (XRD)	491:513	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	32	theme	X-ray	491:495	arg1	XRD					510:512	XRD	510:512	XRD	510:512	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	8	33	theme	highest	1276:1282	arg1	biodegradability					1284:1299	the highest biodegradability	1272:1299	the highest biodegradability of 47.36%	1272:1309	CS-CRE5 film showed the highest biodegradability of 47.36%.					
32057859	3	34	theme	Scanning	406:413	arg1	SEM					436:438	SEM	436:438	SEM	436:438	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	34	theme	Scanning	406:413	arg1	microscopy					424:433	Scanning electron microscopy	406:433	Scanning electron microscopy (SEM)	406:439	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	0	35	theme	active	29:34	arg1	film					51:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	3	36	theme	films	658:662	arg1	interaction					612:622	potential interaction	602:622	potential interaction	602:622	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	36	theme	films	658:662	arg1	stability					636:644	thermal stability	628:644	thermal stability	628:644	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	36	theme	films	658:662	arg1	structure					591:599	structure	591:599	structure	591:599	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	0	37	theme	food	117:120	arg1	application					122:132	food application	117:132	food application	117:132	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	3	38	theme	Fourier	442:448	arg1	FT-IR					483:487	FT-IR	483:487	FT-IR	483:487	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	38	theme	Fourier	442:448	arg1	spectroscopy					469:480	Fourier transform-infrared spectroscopy	442:480	Fourier transform-infrared spectroscopy (FT-IR)	442:488	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	39	theme	electron	415:422	arg1	SEM					436:438	SEM	436:438	SEM	436:438	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	39	theme	electron	415:422	arg1	microscopy					424:433	Scanning electron microscopy	406:433	Scanning electron microscopy (SEM)	406:439	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	40	theme	thermal	519:525	arg1	behavior					527:534	thermal behavior	519:534	thermal behavior analysis (DSC & TGA)	519:555	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	0	41	theme	packaging	41:49	arg1	film					51:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film	0:54	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	9	42	theme	CRE	1346:1348	arg1	addition					1334:1341	addition	1334:1341	addition of CRE into CS	1334:1356	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	1	43	dep	based	199:203	arg1	CS					195:196	CS	195:196	CS	195:196	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	43	dep	based	199:203	arg1	chitosan					185:192	chitosan	185:192	chitosan (CS)	185:197	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	3	44	theme	behavior	527:534	arg1	TGA					552:554	DSC & TGA	546:554	DSC & TGA	546:554	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	44	theme	behavior	527:534	arg1	analysis					536:543	thermal behavior analysis	519:543	thermal behavior analysis (DSC & TGA)	519:555	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	6	45	theme	water	945:949	arg1	solubility					951:960	The water solubility	941:960	The water solubility	941:960	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	6	46	dep	7.81 × 10-11 g·m-1s-1Pa-1	1084:1108	arg1	to					1081:1082	to	1081:1082	to	1081:1082	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	1	47	theme	solution	278:285	arg1	method					295:300	solution casting method	278:300	solution casting method	278:300	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	7	48	theme	ABTS	1134:1137	arg1	ability					1158:1164	ABTS radical scavenging ability	1134:1164	ABTS radical scavenging ability	1134:1164	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	4	49	theme	agglomerates	749:760	arg1	formation					736:744	the formation	732:744	the formation of agglomerates	732:760	It was revealed by SEM that higher extract concentration triggered the formation of agglomerates within the films.					
32057859	5	50	dep	decrease	821:828	arg1	properties					838:847	tensile properties	830:847	decrease tensile properties of the films from 28.9 to 15.4 MPa	821:882	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	2	51	theme	different	310:318	arg1	concentrations					320:333	different concentrations	310:333	different concentrations (1, 3 and 5% in w/w)	310:354	CRE at different concentrations (1, 3 and 5% in w/w) were incorporated into the film-forming solution.					
32057859	8	52	theme	%	1309:1309	arg1	biodegradability					1284:1299	the highest biodegradability	1272:1299	the highest biodegradability of 47.36%	1272:1309	CS-CRE5 film showed the highest biodegradability of 47.36%.					
32057859	1	53	theme	casting	287:293	arg1	method					295:300	solution casting method	278:300	solution casting method	278:300	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	2	54	from	%	346:346	arg1	w/w					351:353	w/w	351:353	w/w	351:353	CRE at different concentrations (1, 3 and 5% in w/w) were incorporated into the film-forming solution.					
32057859	9	55	theme	good	1368:1371	arg1	activity					1403:1410	good antioxidant and antimicrobial activity	1368:1410	good antioxidant and antimicrobial activity	1368:1410	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	5	56	from	28.9	867:870	arg1	properties					838:847	tensile properties	830:847	decrease tensile properties of the films from 28.9 to 15.4 MPa	821:882	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	5	56	from	28.9	867:870	arg1	films					856:860	the films	852:860	the films from 28.9 to 15.4 MPa	852:882	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	7	57	dep	47.05	1210:1214	arg1	to					1207:1208	to	1207:1208	to	1207:1208	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	9	58	theme	food	1495:1498	arg1	industry					1500:1507	food industry	1495:1507	food industry	1495:1507	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	4	59	theme	extract	700:706	arg1	concentration					708:720	higher extract concentration	693:720	higher extract concentration	693:720	It was revealed by SEM that higher extract concentration triggered the formation of agglomerates within the films.					
32057859	3	60	theme	DSC	546:548	arg1	TGA					552:554	DSC & TGA	546:554	DSC & TGA	546:554	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	60	theme	DSC	546:548	arg1	analysis					536:543	thermal behavior analysis	519:543	thermal behavior analysis (DSC & TGA)	519:555	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	7	61	theme	CS-CRE	1169:1174	arg1	films					1176:1180	CS-CRE films	1169:1180	CS-CRE films	1169:1180	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	9	62	theme	food	1467:1470	arg1	material					1482:1489	bio-composite food packaging material	1453:1489	bio-composite food packaging material	1453:1489	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	9	62	theme	food	1467:1470	arg1	it					1428:1429	it	1428:1429	it	1428:1429	The films prepared by addition of CRE into CS exhibited good antioxidant and antimicrobial activity indicating that it could be developed as bio-composite food packaging material for food industry.					
32057859	1	63	theme	based	199:203	arg1	films					222:226	chitosan (CS) based novel functional films	185:226	chitosan (CS) based novel functional films containing Chinese chive root extract (CRE)	185:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	6	64	dep	%	1069:1069	arg1	to					1062:1063	to	1062:1063	to	1062:1063	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	5	65	theme	tensile	830:836	arg1	properties					838:847	tensile properties	830:847	decrease tensile properties of the films from 28.9 to 15.4 MPa	821:882	Incorporation of CRE into CS resulted in decrease tensile properties of the films from 28.9 to 15.4 MPa, whereas thickness was increased from 0.076 to 0.113 mm.					
32057859	3	66	theme	&	550:550	arg1	TGA					552:554	DSC & TGA	546:554	DSC & TGA	546:554	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	66	theme	&	550:550	arg1	analysis					536:543	thermal behavior analysis	519:543	thermal behavior analysis (DSC & TGA)	519:555	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	7	67	theme	films	1176:1180	arg1	ability					1158:1164	ABTS radical scavenging ability	1134:1164	ABTS radical scavenging ability	1134:1164	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	7	67	theme	films	1176:1180	arg1	DPPH					1125:1128	DPPH	1125:1128	DPPH	1125:1128	DPPH and ABTS radical scavenging ability of CS-CRE films were increased from 6.95 to 47.05% and 11.98 to 57.38%, respectively.					
32057859	1	68	theme	novel	205:209	arg1	films					222:226	chitosan (CS) based novel functional films	185:226	chitosan (CS) based novel functional films containing Chinese chive root extract (CRE)	185:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	6	69	theme	swelling	963:970	arg1	degree					972:977	swelling degree	963:977	swelling degree	963:977	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	3	70	theme	transform-infrared	450:467	arg1	FT-IR					483:487	FT-IR	483:487	FT-IR	483:487	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	3	70	theme	transform-infrared	450:467	arg1	spectroscopy					469:480	Fourier transform-infrared spectroscopy	442:480	Fourier transform-infrared spectroscopy (FT-IR)	442:488	Scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal behavior analysis (DSC & TGA) were performed to investigate the structure, potential interaction and thermal stability of prepared films.					
32057859	6	71	theme	water	983:987	arg1	permeability					995:1006	water vapor permeability	983:1006	water vapor permeability	983:1006	The water solubility, swelling degree and water vapor permeability were significantly decreased from 31.6 to 18.7%, 57.4 to 40.5% and 15.67 to 7.81 × 10-11 g·m-1s-1Pa-1, respectively.					
32057859	1	72	theme	functional	211:220	arg1	films					222:226	chitosan (CS) based novel functional films	185:226	chitosan (CS) based novel functional films containing Chinese chive root extract (CRE)	185:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	0	73	theme	chive	75:79	arg1	extract					105:111	Chinese chive (Allium tuberosum) root extract	67:111	Chinese chive (Allium tuberosum) root extract	67:111	Chitosan-based biodegradable active food packaging film containing Chinese chive (Allium tuberosum) root extract for food application.					
32057859	1	74	contain	containing	228:237	arg2	extract					258:264	Chinese chive root extract	239:264	Chinese chive root extract (CRE)	239:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	74	contain	containing	228:237	arg1	films					222:226	chitosan (CS) based novel functional films	185:226	chitosan (CS) based novel functional films containing Chinese chive root extract (CRE)	185:270	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	1	74	contain	containing	228:237	arg2	CRE					267:269	CRE	267:269	CRE	267:269	The objective of the present study was to develop chitosan (CS) based novel functional films containing Chinese chive root extract (CRE) using solution casting method.					
32057859	2	75	theme	film-forming	383:394	arg1	solution					396:403	the film-forming solution	379:403	the film-forming solution	379:403	CRE at different concentrations (1, 3 and 5% in w/w) were incorporated into the film-forming solution.					
32488610	3	0	theme	conditions	500:509	arg1	effects					480:486	The effects	476:486	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His	476:552	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	0	1	theme	Nanocrystalline	87:101	arg1	Cellulose					103:111	Magnetic Nanocrystalline Cellulose	78:111	Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents	78:137	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	1	2	theme	magnetic	169:176	arg1	MNCC					208:211	PA@MNCC	205:211	PA@MNCC	205:211	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	1	2	theme	magnetic	169:176	arg1	cellulose					194:202	magnetic nanocrystalline cellulose	169:202	magnetic nanocrystalline cellulose (PA@MNCC)	169:212	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	0	3	theme	Deep	116:119	arg1	Solvents					130:137	Deep Eutectic Solvents	116:137	Deep Eutectic Solvents	116:137	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	3	4	theme	catalytic	534:542	arg1	Z-Ala-His					544:552	papain catalytic Z-Ala-His	527:552	papain catalytic Z-Ala-His	527:552	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	1	5	theme	efficient	260:268	arg1	Papain					140:145	Papain	140:145	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC)	140:212	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	1	5	theme	efficient	260:268	arg1	biocatalyst					270:280	an efficient biocatalyst	257:280	an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide	257:362	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	1	6	theme	dipeptide	354:362	arg1	synthesis					290:298	the synthesis	286:298	the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide	286:362	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	3	7	theme	Z-Ala-His	544:552	arg1	yield					518:522	the yield	514:522	the yield of papain catalytic Z-Ala-His	514:552	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	5	8	theme	@	786:786	arg1	composite					792:800	the PA@MNCC composite	780:800	the PA@MNCC composite	780:800	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	1	9	theme	nanocrystalline	178:192	arg1	MNCC					208:211	PA@MNCC	205:211	PA@MNCC	205:211	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	1	9	theme	nanocrystalline	178:192	arg1	cellulose					194:202	magnetic nanocrystalline cellulose	169:202	magnetic nanocrystalline cellulose (PA@MNCC)	169:212	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	0	10	theme	Eutectic	121:128	arg1	Solvents					130:137	Deep Eutectic Solvents	116:137	Deep Eutectic Solvents	116:137	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	5	11	from	dipeptide	858:866	arg1	DESs					871:874	DESs	871:874	DESs	871:874	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	3	12	theme	papain	527:532	arg1	Z-Ala-His					544:552	papain catalytic Z-Ala-His	527:552	papain catalytic Z-Ala-His	527:552	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	5	13	theme	MNCC	787:790	arg1	composite					792:800	the PA@MNCC composite	780:800	the PA@MNCC composite	780:800	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	3	14	from	effects	480:486	arg1	yield					518:522	the yield	514:522	the yield of papain catalytic Z-Ala-His	514:552	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	5	15	from	biosynthesis	842:853	arg1	DESs					871:874	DESs	871:874	DESs	871:874	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	4	16	theme	MNCC	686:689	arg1	composite					691:699	this novel PA@MNCC composite	672:699	this novel PA@MNCC composite	672:699	Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces.					
32488610	2	17	theme	eutectic	382:389	arg1	DESs					401:404	DESs	401:404	DESs	401:404	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	2	17	theme	eutectic	382:389	arg1	solvents					391:398	deep eutectic solvents	377:398	deep eutectic solvents (DESs)	377:405	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	2	17	theme	eutectic	382:389	arg1	media					419:423	reaction media	410:423	reaction media	410:423	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	0	18	theme	Dipeptide	33:41	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of Alanyl-Histidine Dipeptide	0:41	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	1	19	theme	@	207:207	arg1	MNCC					208:211	PA@MNCC	205:211	PA@MNCC	205:211	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	1	19	theme	@	207:207	arg1	cellulose					194:202	magnetic nanocrystalline cellulose	169:202	magnetic nanocrystalline cellulose (PA@MNCC)	169:212	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	0	20	theme	Alanyl-Histidine	16:31	arg1	Dipeptide					33:41	Alanyl-Histidine Dipeptide	16:41	Alanyl-Histidine Dipeptide	16:41	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	1	21	dep	-alanyl-histidine	324:340	arg1	Z-Ala-His					343:351	Z-Ala-His	343:351	Z-Ala-His	343:351	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	0	22	from	Cellulose	103:111	arg1	Solvents					130:137	Deep Eutectic Solvents	116:137	Deep Eutectic Solvents	116:137	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	5	23	theme	efficient	832:840	arg1	biosynthesis					842:853	efficient biosynthesis	832:853	efficient biosynthesis of dipeptide in DESs	832:874	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	2	24	theme	reaction	410:417	arg1	solvents					391:398	deep eutectic solvents	377:398	deep eutectic solvents (DESs)	377:405	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	2	24	theme	reaction	410:417	arg1	media					419:423	reaction media	410:423	reaction media	410:423	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	3	25	theme	free	642:645	arg1	papain					647:652	free papain	642:652	free papain (63.3%)	642:660	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	3	25	theme	free	642:645	arg1	%					659:659	63.3%	655:659	63.3%	655:659	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	1	26	theme	PA	205:206	arg1	MNCC					208:211	PA@MNCC	205:211	PA@MNCC	205:211	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	1	26	theme	PA	205:206	arg1	cellulose					194:202	magnetic nanocrystalline cellulose	169:202	magnetic nanocrystalline cellulose (PA@MNCC)	169:212	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	2	27	theme	dipeptide	465:473	arg1	synthesis					438:446	the synthesis	434:446	the synthesis of the Z-Ala-His dipeptide	434:473	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	5	28	theme	PA	784:785	arg1	composite					792:800	the PA@MNCC composite	780:800	the PA@MNCC composite	780:800	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	2	29	theme	Z-Ala-His	455:463	arg1	dipeptide					465:473	the Z-Ala-His dipeptide	451:473	the Z-Ala-His dipeptide	451:473	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	5	30	theme	great	812:816	arg1	potential					818:826	great potential	812:826	great potential for efficient biosynthesis of dipeptide in DESs	812:874	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	4	31	theme	novel	677:681	arg1	composite					691:699	this novel PA@MNCC composite	672:699	this novel PA@MNCC composite	672:699	Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces.					
32488610	3	32	theme	highest	596:602	arg1	yield					604:608	the highest yield	592:608	the highest yield	592:608	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	3	32	theme	highest	596:602	arg1	higher					630:635	higher	630:635	higher	630:635	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	3	33	theme	reaction	491:498	arg1	conditions					500:509	reaction conditions	491:509	reaction conditions	491:509	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	4	34	theme	@	685:685	arg1	composite					691:699	this novel PA@MNCC composite	672:699	this novel PA@MNCC composite	672:699	Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces.					
32488610	4	35	theme	reaction	733:740	arg1	system					742:747	the reaction system	729:747	the reaction system	729:747	Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces.					
32488610	4	36	theme	PA	683:684	arg1	composite					691:699	this novel PA@MNCC composite	672:699	this novel PA@MNCC composite	672:699	Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces.					
32488610	5	37	from	DESs	871:874	arg1	biosynthesis					842:853	efficient biosynthesis	832:853	efficient biosynthesis of dipeptide in DESs	832:874	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	5	38	theme	dipeptide	858:866	arg1	biosynthesis					842:853	efficient biosynthesis	832:853	efficient biosynthesis of dipeptide in DESs	832:874	In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.					
32488610	0	39	theme	Magnetic	78:85	arg1	Cellulose					103:111	Magnetic Nanocrystalline Cellulose	78:111	Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents	78:137	Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.					
32488610	4	40	theme	magnetic	752:759	arg1	forces					761:766	magnetic forces	752:766	magnetic forces	752:766	Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces.					
32488610	2	41	theme	deep	377:380	arg1	DESs					401:404	DESs	401:404	DESs	401:404	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	2	41	theme	deep	377:380	arg1	solvents					391:398	deep eutectic solvents	377:398	deep eutectic solvents (DESs)	377:405	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	2	41	theme	deep	377:380	arg1	media					419:423	reaction media	410:423	reaction media	410:423	Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide.					
32488610	1	42	theme	-alanyl-histidine	324:340	arg1	dipeptide					354:362	N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide	303:362	N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide	303:362	Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide.					
32488610	3	43	theme	%	617:617	arg1	yield					604:608	the highest yield	592:608	the highest yield	592:608	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32488610	3	43	theme	%	617:617	arg1	higher					630:635	higher	630:635	higher	630:635	The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%).					
32311206	0	0	theme	Fruit	90:94	arg1	Tissues					96:102	Fruit Tissues	90:102	Fruit Tissues	90:102	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	2	1	theme	citrus	347:352	arg1	growth					360:365	citrus fruit growth	347:365	citrus fruit growth	347:365	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	5	2	theme	hesperidin	680:689	arg1	contents					668:675	the contents	664:675	the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin	664:732	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	5	3	theme	eriodictyol	701:711	arg1	contents					668:675	the contents	664:675	the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin	664:732	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	6	4	theme	citrus	916:921	arg1	peel					923:926	citrus peel	916:926	citrus peel	916:926	The antioxidant activities in citrus peel and pulp were found to be significantly correlated with some flavonoids.					
32311206	4	5	from	pulp	555:558	arg1	amounts					512:518	The amounts	508:518	The amounts of main flavonoids in the peel and pulp	508:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	4	5	from	pulp	555:558	arg1	flavonoids					528:537	main flavonoids	523:537	main flavonoids in the peel and pulp	523:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	2	6	from	evolution	306:314	arg1	flavonoids					287:296	flavonoids	287:296	flavonoids	287:296	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	5	7	theme	fruits	812:817	arg1	growth					786:791	growth	786:791	growth	786:791	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	5	7	theme	fruits	812:817	arg1	development					797:807	development	797:807	development	797:807	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	7	8	theme	guiding	1023:1029	arg1	values					1031:1036	guiding values	1023:1036	guiding values	1023:1036	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	4	9	from	peel	546:549	arg1	amounts					512:518	The amounts	508:518	The amounts of main flavonoids in the peel and pulp	508:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	4	9	from	peel	546:549	arg1	flavonoids					528:537	main flavonoids	523:537	main flavonoids in the peel and pulp	523:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	7	10	theme	citrus	1078:1083	arg1	by-products					1091:1101	citrus fruit by-products	1078:1101	citrus fruit by-products	1078:1101	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	3	11	theme	30 days	499:505	arg1	intervals					486:494	intervals	486:494	intervals of 30 days	486:505	In this study, fruits of five citrus cultivars cultivated in China were sampled at 60-210 days post-anthesis (DPA) at intervals of 30 days.					
32311206	3	12	theme	citrus	398:403	arg1	cultivars					405:413	five citrus cultivars	393:413	five citrus cultivars cultivated in China	393:433	In this study, fruits of five citrus cultivars cultivated in China were sampled at 60-210 days post-anthesis (DPA) at intervals of 30 days.					
32311206	0	13	theme	Developmental	108:120	arg1	Stages					122:127	Developmental Stages	108:127	Developmental Stages	108:127	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	4	14	from	amounts	512:518	arg1	pulp					555:558	pulp	555:558	pulp	555:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	4	14	from	amounts	512:518	arg1	peel					546:549	peel	546:549	peel	546:549	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	7	15	theme	by-products	1091:1101	arg1	utilization					1063:1073	utilization	1063:1073	utilization	1063:1073	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	7	15	theme	by-products	1091:1101	arg1	production					1048:1057	citrus production	1041:1057	citrus production	1041:1057	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	1	16	theme	active	178:183	arg1	compounds					185:193	biologically active compounds	165:193	biologically active compounds	165:193	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	7	17	theme	fruit	1085:1089	arg1	by-products					1091:1101	citrus fruit by-products	1078:1101	citrus fruit by-products	1078:1101	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	6	18	theme	antioxidant	890:900	arg1	activities					902:911	The antioxidant activities	886:911	The antioxidant activities in citrus peel and pulp	886:935	The antioxidant activities in citrus peel and pulp were found to be significantly correlated with some flavonoids.					
32311206	1	19	theme	compounds	185:193	arg1	number					155:160	A large number	147:160	A large number of biologically active compounds	147:193	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	19	theme	compounds	185:193	arg1	present					199:205	present	199:205	present	199:205	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	4	20	from	flavonoids	528:537	arg1	pulp					555:558	pulp	555:558	pulp	555:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	4	20	from	flavonoids	528:537	arg1	peel					546:549	peel	546:549	peel	546:549	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	4	21	theme	main	523:526	arg1	flavonoids					528:537	main flavonoids	523:537	main flavonoids in the peel and pulp	523:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	3	22	theme	cultivars	405:413	arg1	fruits					383:388	fruits	383:388	fruits of five citrus cultivars cultivated in China	383:433	In this study, fruits of five citrus cultivars cultivated in China were sampled at 60-210 days post-anthesis (DPA) at intervals of 30 days.					
32311206	0	23	theme	Composition	24:34	arg1	Variations					0:9	Variations	0:9	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.	0:145	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	0	24	theme	Citrus	132:137	arg1	Fruits					139:144	Citrus Fruits	132:144	Citrus Fruits	132:144	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	5	25	dep	growth	786:791	arg1	the					782:784	the	782:784	the	782:784	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	0	26	theme	Flavonoid	14:22	arg1	Composition					24:34	Flavonoid Composition	14:34	Flavonoid Composition	14:34	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	0	27	theme	Antioxidant	40:50	arg1	Properties					52:61	Antioxidant Properties	40:61	Antioxidant Properties	40:61	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	4	28	theme	flavonoids	528:537	arg1	amounts					512:518	The amounts	508:518	The amounts of main flavonoids in the peel and pulp	508:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	4	28	theme	flavonoids	528:537	arg1	flavonoids					528:537	main flavonoids	523:537	main flavonoids in the peel and pulp	523:558	The amounts of main flavonoids in the peel and pulp were analyzed by HPLC and their activities were studied by DPPH, ABTS and FRAP.					
32311206	1	29	theme	ripe	210:213	arg1	fruits					222:227	ripe citrus fruits	210:227	ripe citrus fruits	210:227	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	5	30	theme	rutin	714:718	arg1	contents					668:675	the contents	664:675	the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin	664:732	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	0	31	theme	Fruits	139:144	arg1	Cultivars					79:87	Different Cultivars	69:87	Different Cultivars	69:87	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	0	31	theme	Fruits	139:144	arg1	Tissues					96:102	Fruit Tissues	90:102	Fruit Tissues	90:102	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	0	31	theme	Fruits	139:144	arg1	Stages					122:127	Developmental Stages	108:127	Developmental Stages	108:127	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	5	32	theme	opposite	827:834	arg1	tendency					836:843	an opposite tendency	824:843	an opposite tendency	824:843	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	1	33	theme	citrus	215:220	arg1	fruits					222:227	ripe citrus fruits	210:227	ripe citrus fruits	210:227	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	7	34	theme	citrus	1041:1046	arg1	production					1048:1057	citrus production	1041:1057	citrus production	1041:1057	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	5	35	theme	nobiletin	724:732	arg1	contents					668:675	the contents	664:675	the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin	664:732	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	5	36	theme	diosmin	692:698	arg1	contents					668:675	the contents	664:675	the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin	664:732	The results showed that the contents of hesperidin, diosmin, eriodictyol, rutin and nobiletin increased before 90 DPA and then decreased with the growth and development of fruits, but an opposite tendency was observed for naringin and narirutin.					
32311206	2	37	theme	activity	331:338	arg1	evolution					306:314	the evolution	302:314	the evolution of antioxidant activity during citrus fruit growth	302:365	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	2	37	theme	activity	331:338	arg1	changes					276:282	the changes	272:282	the changes in flavonoids	272:296	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	0	38	theme	Properties	52:61	arg1	Variations					0:9	Variations	0:9	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.	0:145	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	7	39	from	values	1031:1036	arg1	utilization					1063:1073	utilization	1063:1073	utilization	1063:1073	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	7	39	from	values	1031:1036	arg1	production					1048:1057	citrus production	1041:1057	citrus production	1041:1057	The results may be of guiding values in citrus production and utilization of citrus fruit by-products.					
32311206	1	40	located	present	199:205	arg1	fruits					222:227	ripe citrus fruits	210:227	ripe citrus fruits	210:227	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	40	located	present	199:205	arg2	number					155:160	A large number	147:160	A large number of biologically active compounds	147:193	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	40	located	present	199:205	arg2	present					199:205	present	199:205	present	199:205	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	41	from	present	199:205	arg1	fruits					222:227	ripe citrus fruits	210:227	ripe citrus fruits	210:227	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	2	42	theme	antioxidant	319:329	arg1	activity					331:338	antioxidant activity	319:338	antioxidant activity	319:338	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	1	43	from	fruits	222:227	arg1	number					155:160	A large number	147:160	A large number of biologically active compounds	147:193	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	43	from	fruits	222:227	arg1	present					199:205	present	199:205	present	199:205	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	44	attach	present	199:205	arg1	fruits					222:227	ripe citrus fruits	210:227	ripe citrus fruits	210:227	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	44	attach	present	199:205	arg2	number					155:160	A large number	147:160	A large number of biologically active compounds	147:193	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	44	attach	present	199:205	arg2	present					199:205	present	199:205	present	199:205	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	3	45	theme	60-210 days	451:461	arg1	DPA					478:480	DPA	478:480	DPA	478:480	In this study, fruits of five citrus cultivars cultivated in China were sampled at 60-210 days post-anthesis (DPA) at intervals of 30 days.					
32311206	3	45	theme	60-210 days	451:461	arg1	post-anthesis					463:475	60-210 days post-anthesis	451:475	60-210 days post-anthesis (DPA)	451:481	In this study, fruits of five citrus cultivars cultivated in China were sampled at 60-210 days post-anthesis (DPA) at intervals of 30 days.					
32311206	6	46	from	activities	902:911	arg1	pulp					932:935	pulp	932:935	pulp	932:935	The antioxidant activities in citrus peel and pulp were found to be significantly correlated with some flavonoids.					
32311206	6	46	from	activities	902:911	arg1	peel					923:926	citrus peel	916:926	citrus peel	916:926	The antioxidant activities in citrus peel and pulp were found to be significantly correlated with some flavonoids.					
32311206	1	47	theme	large	149:153	arg1	number					155:160	A large number	147:160	A large number of biologically active compounds	147:193	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	1	47	theme	large	149:153	arg1	present					199:205	present	199:205	present	199:205	A large number of biologically active compounds are present in ripe citrus fruits.					
32311206	2	48	from	changes	276:282	arg1	flavonoids					287:296	flavonoids	287:296	flavonoids	287:296	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	0	49	theme	Different	69:77	arg1	Cultivars					79:87	Different Cultivars	69:87	Different Cultivars	69:87	Variations of Flavonoid Composition and Antioxidant Properties among Different Cultivars, Fruit Tissues and Developmental Stages of Citrus Fruits.					
32311206	2	50	theme	few	239:241	arg1	studies					243:249	few studies	239:249	few studies	239:249	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
32311206	2	51	theme	fruit	354:358	arg1	growth					360:365	citrus fruit growth	347:365	citrus fruit growth	347:365	However, few studies have been focused on the changes in flavonoids and the evolution of antioxidant activity during citrus fruit growth.					
33289771	5	0	theme	sensor	773:778	arg1	behaviors					748:756	ECL behaviors	744:756	ECL behaviors	744:756	Electrochemical and ECL behaviors of the MIP-ECL sensor were evaluated.					
33289771	5	0	theme	sensor	773:778	arg1	Electrochemical					724:738	Electrochemical	724:738	Electrochemical	724:738	Electrochemical and ECL behaviors of the MIP-ECL sensor were evaluated.					
33289771	4	1	theme	Ru	712:713	arg1	32+					719:721	Ru(bpy)32+	712:721	Ru(bpy)32+	712:721	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	11	2	theme	human	1559:1563	arg1	serum					1565:1569	human serum and urine samples	1559:1587	serum	1565:1569	Ultimately, this sensor was utilized successfully for the analysis of TRI in human serum and urine samples without any special pretreatment.					
33289771	3	3	theme	facile	501:506	arg1	process					521:527	a facile sonochemical process	499:527	a facile sonochemical process	499:527	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO) was synthesized through a facile sonochemical process.					
33289771	10	4	theme	precise	1425:1431	arg1	detection					1433:1441	precise detection	1425:1441	precise detection of TRI in complex biological matrices	1425:1479	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	4	5	theme	electrode	586:594	arg1	surface					572:578	the surface	568:578	the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+	568:721	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	8	6	theme	pM	1166:1167	arg1	range					1149:1153	the range	1145:1153	the range of 0.2-100 pM with a 0.995 correlation coefficient	1145:1204	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	10	7	theme	<	1369:1369	arg1	%					1374:1374	RSDs < 3.4%	1364:1374	RSDs < 3.4%	1364:1374	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	7	theme	<	1369:1369	arg1	urine					1357:1361	human urine	1351:1361	human urine (RSDs < 3.4%)	1351:1375	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	3	8	theme	sonochemical	508:519	arg1	process					521:527	a facile sonochemical process	499:527	a facile sonochemical process	499:527	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO) was synthesized through a facile sonochemical process.					
33289771	8	9	theme	0.995	1176:1180	arg1	coefficient					1194:1204	a 0.995 correlation coefficient	1174:1204	a 0.995 correlation coefficient	1174:1204	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	10	10	theme	RSDs	1364:1367	arg1	%					1374:1374	RSDs < 3.4%	1364:1374	RSDs < 3.4%	1364:1374	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	10	theme	RSDs	1364:1367	arg1	urine					1357:1361	human urine	1351:1361	human urine (RSDs < 3.4%)	1351:1375	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	8	11	with	pM	1166:1167	arg1	coefficient					1194:1204	a 0.995 correlation coefficient	1174:1204	a 0.995 correlation coefficient	1174:1204	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	0	12	theme	ultratrace	67:76	arg1	determination					78:90	ultratrace determination	67:90	ultratrace determination of trimipramine	67:106	A novel MIP-ECL sensor based on RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine.					
33289771	10	13	theme	human	1307:1311	arg1	%					1330:1330	RSDs < 4.1%	1320:1330	RSDs < 4.1%	1320:1330	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	13	theme	human	1307:1311	arg1	serum					1313:1317	human serum	1307:1317	human serum (RSDs < 4.1%)	1307:1331	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	6	14	theme	ECL	813:815	arg1	intensity					817:825	the ECL intensity	809:825	the ECL intensity	809:825	Accordingly, the ECL intensity was significantly enhanced via TRI molecule adsorption on the MIP composite film.					
33289771	4	15	theme	bpy	715:717	arg1	32+					719:721	Ru(bpy)32+	712:721	Ru(bpy)32+	712:721	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	8	16	from	linear	1135:1140	arg1	range					1149:1153	the range	1145:1153	the range of 0.2-100 pM with a 0.995 correlation coefficient	1145:1204	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	0	17	theme	trimipramine	95:106	arg1	determination					78:90	ultratrace determination	67:90	ultratrace determination of trimipramine	67:106	A novel MIP-ECL sensor based on RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine.					
33289771	4	18	used	utilized	545:552	arg2	CeO2NP-RGO					530:539	CeO2NP-RGO	530:539	CeO2NP-RGO	530:539	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	10	19	theme	TRI	1446:1448	arg1	detection					1433:1441	precise detection	1425:1441	precise detection of TRI in complex biological matrices	1425:1479	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	3	20	theme	graphene	426:433	arg1	oxide					435:439	reduced graphene oxide	418:439	reduced graphene oxide decorated with ceria (CeO2NP-RGO)	418:473	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO) was synthesized through a facile sonochemical process.					
33289771	8	21	theme	correlation	1182:1192	arg1	coefficient					1194:1204	a 0.995 correlation coefficient	1174:1204	a 0.995 correlation coefficient	1174:1204	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	6	22	theme	composite	893:901	arg1	film					903:906	the MIP composite film	885:906	the MIP composite film	885:906	Accordingly, the ECL intensity was significantly enhanced via TRI molecule adsorption on the MIP composite film.					
33289771	8	23	from	range	1149:1153	arg1	linear					1135:1140	linear	1135:1140	linear	1135:1140	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	8	23	from	range	1149:1153	arg1	response					1100:1107	The assays response	1089:1107	The assays response for TRI concentration	1089:1129	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	6	24	theme	molecule	862:869	arg1	adsorption					871:880	TRI molecule adsorption	858:880	TRI molecule adsorption	858:880	Accordingly, the ECL intensity was significantly enhanced via TRI molecule adsorption on the MIP composite film.					
33289771	1	25	theme	-electrochemiluminescence	152:176	arg1	sensor					188:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor	109:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	109:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	0	26	theme	novel	2:6	arg1	sensor					16:21	A novel MIP-ECL sensor	0:21	A novel MIP-ECL sensor	0:21	A novel MIP-ECL sensor based on RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine.					
33289771	6	27	theme	MIP	889:891	arg1	film					903:906	the MIP composite film	885:906	the MIP composite film	885:906	Accordingly, the ECL intensity was significantly enhanced via TRI molecule adsorption on the MIP composite film.					
33289771	10	28	theme	human	1351:1355	arg1	%					1374:1374	RSDs < 3.4%	1364:1374	RSDs < 3.4%	1364:1374	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	28	theme	human	1351:1355	arg1	urine					1357:1361	human urine	1351:1361	human urine (RSDs < 3.4%)	1351:1375	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	2	29	theme	polymerization	375:388	arg1	process					390:396	the precipitation polymerization process	357:396	the precipitation polymerization process	357:396	TRI-MIP was synthesized via the precipitation polymerization process.					
33289771	10	30	used	used	1416:1419	arg2	sensor					1402:1407	the MIP-ECL sensor	1390:1407	the MIP-ECL sensor	1390:1407	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	8	31	theme	assays	1093:1098	arg1	linear					1135:1140	linear	1135:1140	linear	1135:1140	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	8	31	theme	assays	1093:1098	arg1	response					1100:1107	The assays response	1089:1107	The assays response for TRI concentration	1089:1129	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	3	32	theme	oxide	435:439	arg1	nanocomposite					401:413	A nanocomposite	399:413	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO)	399:473	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO) was synthesized through a facile sonochemical process.					
33289771	3	32	theme	oxide	435:439	arg1	oxide					435:439	reduced graphene oxide	418:439	reduced graphene oxide decorated with ceria (CeO2NP-RGO)	418:473	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO) was synthesized through a facile sonochemical process.					
33289771	1	33	theme	ultrasensitive	262:275	arg1	detection					296:304	the ultrasensitive and ultraselective detection	258:304	the ultrasensitive and ultraselective detection of trimipramine (TRI)	258:326	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	8	34	theme	TRI	1113:1115	arg1	concentration					1117:1129	TRI concentration	1113:1129	TRI concentration	1113:1129	The assays response for TRI concentration was linear in the range of 0.2-100 pM with a 0.995 correlation coefficient.					
33289771	7	35	theme	high	950:953	arg1	sensitivity					955:965	high sensitivity	950:965	high sensitivity	950:965	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	11	36	used	utilized	1510:1517	arg2	sensor					1499:1504	this sensor	1494:1504	this sensor	1494:1504	Ultimately, this sensor was utilized successfully for the analysis of TRI in human serum and urine samples without any special pretreatment.					
33289771	0	37	theme	MIP-ECL	8:14	arg1	sensor					16:21	A novel MIP-ECL sensor	0:21	A novel MIP-ECL sensor	0:21	A novel MIP-ECL sensor based on RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine.					
33289771	1	38	theme	MIP	148:150	arg1	sensor					188:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor	109:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	109:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	4	39	theme	electrical	635:644	arg1	conductivity					646:657	an excellent electrical conductivity	622:657	an excellent electrical conductivity	622:657	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	10	40	theme	biological	1461:1470	arg1	matrices					1472:1479	complex biological matrices	1453:1479	complex biological matrices	1453:1479	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	1	41	theme	ultraselective	281:294	arg1	detection					296:304	the ultrasensitive and ultraselective detection	258:304	the ultrasensitive and ultraselective detection of trimipramine (TRI)	258:326	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	10	42	theme	MIP-ECL	1394:1400	arg1	sensor					1402:1407	the MIP-ECL sensor	1390:1407	the MIP-ECL sensor	1390:1407	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	43	from	detection	1433:1441	arg1	matrices					1472:1479	complex biological matrices	1453:1479	complex biological matrices	1453:1479	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	2	44	theme	precipitation	361:373	arg1	process					390:396	the precipitation polymerization process	357:396	the precipitation polymerization process	357:396	TRI-MIP was synthesized via the precipitation polymerization process.					
33289771	4	45	theme	enhanced	660:667	arg1	electrochemical					669:683	enhanced electrochemical	660:683	enhanced electrochemical	660:683	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	3	46	theme	reduced	418:424	arg1	oxide					435:439	reduced graphene oxide	418:439	reduced graphene oxide decorated with ceria (CeO2NP-RGO)	418:473	A nanocomposite of reduced graphene oxide decorated with ceria (CeO2NP-RGO) was synthesized through a facile sonochemical process.					
33289771	11	47	theme	urine	1575:1579	arg1	samples					1581:1587	human serum and urine samples	1559:1587	samples	1581:1587	Ultimately, this sensor was utilized successfully for the analysis of TRI in human serum and urine samples without any special pretreatment.					
33289771	4	48	theme	excellent	625:633	arg1	conductivity					646:657	an excellent electrical conductivity	622:657	an excellent electrical conductivity	622:657	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	10	49	theme	complex	1453:1459	arg1	matrices					1472:1479	complex biological matrices	1453:1479	complex biological matrices	1453:1479	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	7	50	theme	optimal	1069:1075	arg1	conditions					1077:1086	the applied optimal conditions	1057:1086	the applied optimal conditions	1057:1086	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	5	51	theme	MIP-ECL	765:771	arg1	sensor					773:778	the MIP-ECL sensor	761:778	the MIP-ECL sensor	761:778	Electrochemical and ECL behaviors of the MIP-ECL sensor were evaluated.					
33289771	1	52	theme	MIP-ECL	179:185	arg1	sensor					188:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor	109:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	109:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	4	53	theme	32+	719:721	arg1	conductivity					646:657	an excellent electrical conductivity	622:657	an excellent electrical conductivity	622:657	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	4	53	theme	32+	719:721	arg1	electrochemical					669:683	enhanced electrochemical	660:683	enhanced electrochemical	660:683	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	4	53	theme	32+	719:721	arg1	characteristics					693:707	ECL characteristics	689:707	ECL characteristics	689:707	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	0	54	theme	RGO-CeO2NP/Ru	32:44	arg1	32+-chitosan					50:61	RGO-CeO2NP/Ru(bpy)32+-chitosan	32:61	RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine	32:106	A novel MIP-ECL sensor based on RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine.					
33289771	1	55	theme	trimipramine	309:320	arg1	detection					296:304	the ultrasensitive and ultraselective detection	258:304	the ultrasensitive and ultraselective detection of trimipramine (TRI)	258:326	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	6	56	theme	TRI	858:860	arg1	adsorption					871:880	TRI molecule adsorption	858:880	TRI molecule adsorption	858:880	Accordingly, the ECL intensity was significantly enhanced via TRI molecule adsorption on the MIP composite film.					
33289771	4	57	theme	ECL	689:691	arg1	characteristics					693:707	ECL characteristics	689:707	ECL characteristics	689:707	CeO2NP-RGO was utilized for modifying the surface of an electrode which consequently led to an excellent electrical conductivity, enhanced electrochemical and ECL characteristics of Ru(bpy)32+.					
33289771	7	58	theme	MIP-ECL	922:928	arg1	sensor					930:935	The prepared MIP-ECL sensor	909:935	The prepared MIP-ECL sensor	909:935	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	9	59	theme	detection	1220:1228	arg1	limit					1211:1215	The limit	1207:1215	The limit of detection (LOD)	1207:1234	The limit of detection (LOD) was as small as 0.025 pM (S/N = 3).					
33289771	9	59	theme	detection	1220:1228	arg1	small					1243:1247	small	1243:1247	small	1243:1247	The limit of detection (LOD) was as small as 0.025 pM (S/N = 3).					
33289771	7	60	theme	TRI	1033:1035	arg1	determination					1037:1049	TRI determination	1033:1049	TRI determination	1033:1049	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	1	61	theme	novel	111:115	arg1	sensor					188:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor	109:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	109:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	10	62	theme	RSDs	1320:1323	arg1	%					1330:1330	RSDs < 4.1%	1320:1330	RSDs < 4.1%	1320:1330	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	62	theme	RSDs	1320:1323	arg1	serum					1313:1317	human serum	1307:1317	human serum (RSDs < 4.1%)	1307:1331	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	1	63	theme	CeO2NP-RGO/Ru	204:216	arg1	32+-MIP-chitosan					222:237	CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	204:237	CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	204:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	7	64	theme	applied	1061:1067	arg1	conditions					1077:1086	the applied optimal conditions	1057:1086	the applied optimal conditions	1057:1086	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	11	65	theme	special	1601:1607	arg1	pretreatment					1609:1620	any special pretreatment	1597:1620	any special pretreatment	1597:1620	Ultimately, this sensor was utilized successfully for the analysis of TRI in human serum and urine samples without any special pretreatment.					
33289771	11	66	theme	TRI	1552:1554	arg1	analysis					1540:1547	the analysis	1536:1547	the analysis of TRI	1536:1554	Ultimately, this sensor was utilized successfully for the analysis of TRI in human serum and urine samples without any special pretreatment.					
33289771	10	67	theme	<	1325:1325	arg1	%					1330:1330	RSDs < 4.1%	1320:1330	RSDs < 4.1%	1320:1330	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	10	67	theme	<	1325:1325	arg1	serum					1313:1317	human serum	1307:1317	human serum (RSDs < 4.1%)	1307:1331	The recoveries between 91-107% for human serum (RSDs < 4.1%) and 94-104.6% for human urine (RSDs < 3.4%) approve that the MIP-ECL sensor can be used for precise detection of TRI in complex biological matrices.					
33289771	1	68	theme	imprinted	129:137	arg1	sensor					188:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor	109:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	109:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	7	69	theme	prepared	913:920	arg1	sensor					930:935	The prepared MIP-ECL sensor	909:935	The prepared MIP-ECL sensor	909:935	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	1	70	theme	bpy	218:220	arg1	32+-MIP-chitosan					222:237	CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	204:237	CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	204:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	0	71	theme	bpy	46:48	arg1	32+-chitosan					50:61	RGO-CeO2NP/Ru(bpy)32+-chitosan	32:61	RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine	32:106	A novel MIP-ECL sensor based on RGO-CeO2NP/Ru(bpy)32+-chitosan for ultratrace determination of trimipramine.					
33289771	9	72	theme	=	1266:1266	arg1	pM					1258:1259	0.025 pM	1252:1259	0.025 pM (S/N = 3)	1252:1269	The limit of detection (LOD) was as small as 0.025 pM (S/N = 3).					
33289771	9	72	theme	=	1266:1266	arg1	S/N					1262:1264	S/N = 3	1262:1268	S/N = 3	1262:1268	The limit of detection (LOD) was as small as 0.025 pM (S/N = 3).					
33289771	7	73	theme	good	994:997	arg1	reproducibility					999:1013	reproducibility	999:1013	reproducibility	999:1013	The prepared MIP-ECL sensor demonstrated high sensitivity and selectivity as well as good reproducibility and stability for TRI determination under the applied optimal conditions.					
33289771	1	74	theme	polymer	139:145	arg1	sensor					188:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor	109:193	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan	109:237	A novel molecularly imprinted polymer (MIP)-electrochemiluminescence (MIP-ECL) sensor based on CeO2NP-RGO/Ru(bpy)32+-MIP-chitosan was introduced for the ultrasensitive and ultraselective detection of trimipramine (TRI).					
33289771	5	75	theme	ECL	744:746	arg1	behaviors					748:756	ECL behaviors	744:756	ECL behaviors	744:756	Electrochemical and ECL behaviors of the MIP-ECL sensor were evaluated.					
33112614	2	0	theme	volume	597:602	arg1	shrinkage					604:612	volume shrinkage	597:612	volume shrinkage	597:612	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	8	1	theme	ultrasensitive	1665:1678	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	2	2	theme	reaction	584:591	arg1	treatment					614:622	the controlled silver mirror reaction and volume shrinkage treatment	555:622	the controlled silver mirror reaction and volume shrinkage treatment	555:622	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	4	3	theme	SERS	1049:1052	arg1	sensitivity					1054:1064	the SERS sensitivity	1045:1064	the SERS sensitivity	1045:1064	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	1	4	theme	Ag-NPs	282:287	arg1	BNC					289:291	Ag-NPs@BNC	282:291	Ag-NPs@BNC	282:291	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	8	5	theme	Ag-NPs	1692:1697	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	1	6	theme	@	288:288	arg1	BNC					289:291	Ag-NPs@BNC	282:291	Ag-NPs@BNC	282:291	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	2	7	theme	controlled	559:568	arg1	reaction					584:591	the controlled silver mirror reaction	555:591	the controlled silver mirror reaction	555:591	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	3	8	theme	unique	736:741	arg1	spots					750:754	these unique 3D hot spots	730:754	these unique 3D hot spots	730:754	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	6	9	theme	pollutants	1487:1496	arg1	detection					1460:1468	the rapid and quantitative detection	1433:1468	the rapid and quantitative detection of toxic organic pollutants	1433:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	6	10	theme	toxic	1473:1477	arg1	pollutants					1487:1496	toxic organic pollutants	1473:1496	toxic organic pollutants	1473:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	1	11	theme	hybrid	199:204	arg1	substrate					372:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate	297:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	11	theme	hybrid	199:204	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	11	theme	hybrid	199:204	arg1	composites					206:215	the hybrid composites	195:215	the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	195:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	2	12	dep	reaction	584:591	arg1	mirror					577:582	mirror	577:582	mirror	577:582	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	2	13	theme	networked	531:539	arg1	fibers					545:550	the networked BNC fibers	527:550	the networked BNC fibers	527:550	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	1	14	dep	nanocellulose	256:268	arg1	denoted					271:277	denoted	271:277	denoted as Ag-NPs@BNC	271:291	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	8	15	from	substrate	1749:1757	arg1	identification					1772:1785	the rapid identification	1762:1785	the rapid identification of various organic molecules	1762:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	1	16	theme	ultrahigh	387:395	arg1	sensitivity					402:412	ultrahigh SERS sensitivity	387:412	ultrahigh SERS sensitivity	387:412	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	7	17	theme	varied	1597:1602	arg1	conditions					1614:1623	varied detection conditions	1597:1623	varied detection conditions	1597:1623	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	2	18	theme	silver	570:575	arg1	reaction					584:591	the controlled silver mirror reaction	555:591	the controlled silver mirror reaction	555:591	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	3	19	theme	hot	746:748	arg1	spots					750:754	these unique 3D hot spots	730:754	these unique 3D hot spots	730:754	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	8	20	theme	versatile	1734:1742	arg1	substrate					1749:1757	a versatile SERS substrate	1732:1757	a versatile SERS substrate in the rapid identification of various organic molecules	1732:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	8	20	theme	versatile	1734:1742	arg1	potential					1719:1727	potential	1719:1727	potential	1719:1727	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	3	21	theme	spectral	837:844	arg1	reproducibility					846:860	good spectral reproducibility	832:860	good spectral reproducibility	832:860	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	4	22	theme	good	902:905	arg1	permeability					907:918	good permeability	902:918	good permeability	902:918	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	3	23	theme	@	780:780	arg1	substrates					785:794	the as-presented Ag-NPs@BNC substrates	757:794	the as-presented Ag-NPs@BNC substrates	757:794	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	1	24	theme	excellent	415:423	arg1	reproducibility					432:446	excellent signal reproducibility	415:446	excellent signal reproducibility	415:446	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	4	25	theme	target	973:978	arg1	molecules					980:988	the target molecules	969:988	the target molecules in the highly active hot spot areas	969:1024	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	3	26	theme	as-presented	761:772	arg1	substrates					785:794	the as-presented Ag-NPs@BNC substrates	757:794	the as-presented Ag-NPs@BNC substrates	757:794	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	6	27	theme	linear	1331:1336	arg1	response					1338:1345	The good linear response	1322:1345	The good linear response of the intensity and the logarithmic concentration	1322:1396	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	2	28	with	Ag-NPs	480:485	arg1	density					497:503	high density	492:503	high density	492:503	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	2	29	theme	high	492:495	arg1	density					497:503	high density	492:503	high density	492:503	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	2	30	theme	hybrid	698:703	arg1	substrate					705:713	the 3D networked hybrid substrate	681:713	the 3D networked hybrid substrate	681:713	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	2	31	from	"	676:676	arg1	substrate					705:713	the 3D networked hybrid substrate	681:713	the 3D networked hybrid substrate	681:713	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	3	32	theme	ultrahigh	806:814	arg1	sensitivity					816:826	ultrahigh sensitivity	806:826	ultrahigh sensitivity	806:826	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	0	33	theme	Surface-Enhanced	69:84	arg1	Scattering					92:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering	44:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	1	34	theme	surface-enhanced	331:346	arg1	SERS					366:369	SERS	366:369	SERS	366:369	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	34	theme	surface-enhanced	331:346	arg1	scattering					354:363	surface-enhanced Raman scattering	331:363	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	35	theme	simple	156:161	arg1	approach					173:180	a well-designed, low-cost, and simple synthetic approach	125:180	a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	125:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	7	36	theme	BNC	1535:1537	arg1	substrate					1539:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	5	37	theme	SERS	1240:1243	arg1	substrates					1245:1254	SERS substrates	1240:1254	SERS substrates	1240:1254	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	0	38	theme	Scattering	92:101	arg1	Substrate					103:111	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	1	39	theme	scattering	354:363	arg1	substrate					372:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate	297:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	39	theme	scattering	354:363	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	39	theme	scattering	354:363	arg1	composites					206:215	the hybrid composites	195:215	the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	195:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	8	40	theme	various	1790:1796	arg1	molecules					1806:1814	various organic molecules	1790:1814	various organic molecules	1790:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	7	41	theme	Ag-NPs	1528:1533	arg1	substrate					1539:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	8	42	theme	molecules	1806:1814	arg1	identification					1772:1785	the rapid identification	1762:1785	the rapid identification of various organic molecules	1762:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	2	43	theme	spots	671:675	arg1	"					676:676	"hot spots"	666:676	"hot spots" in the 3D networked hybrid substrate	666:713	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	5	44	theme	10-9	1300:1303	arg1	M					1305:1305	M	1305:1305	M	1305:1305	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	6	45	theme	concentration	1384:1396	arg1	response					1338:1345	The good linear response	1322:1345	The good linear response of the intensity and the logarithmic concentration	1322:1396	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	5	46	theme	organic	1133:1139	arg1	thiram					1183:1188	thiram	1183:1188	thiram	1183:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	46	theme	organic	1133:1139	arg1	pollutants					1141:1150	toxic organic pollutants	1127:1150	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	46	theme	organic	1133:1139	arg1	2-naphthalenethiol					1160:1177	2-naphthalenethiol	1160:1177	2-naphthalenethiol	1160:1177	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	46	theme	organic	1133:1139	arg1	molecules					1093:1101	dye molecules	1089:1101	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	46	theme	organic	1133:1139	arg1	result					1072:1077	a result	1070:1077	a result	1070:1077	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	47	theme	1.6	1279:1281	arg1	×					1283:1283	×	1283:1283	×	1283:1283	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	0	48	theme	Bacterial	17:25	arg1	Nanocellulose					27:39	@Bacterial Nanocellulose	16:39	@Bacterial Nanocellulose	16:39	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	1	49	with	substrate	372:380	arg1	reproducibility					432:446	excellent signal reproducibility	415:446	excellent signal reproducibility	415:446	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	49	with	substrate	372:380	arg1	stability					453:461	stability	453:461	stability	453:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	49	with	substrate	372:380	arg1	sensitivity					402:412	ultrahigh SERS sensitivity	387:412	ultrahigh SERS sensitivity	387:412	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	5	50	theme	10-8	1285:1288	arg1	sensitivities					1262:1274	sensitivities	1262:1274	sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M	1262:1305	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	6	51	theme	intensity	1354:1362	arg1	response					1338:1345	The good linear response	1322:1345	The good linear response of the intensity and the logarithmic concentration	1322:1396	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	4	52	theme	hot	1011:1013	arg1	spot					1015:1018	the highly active hot spot	993:1018	the highly active hot spot areas	993:1024	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	0	53	theme	3D	46:47	arg1	Scattering					92:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering	44:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	6	54	theme	quantitative	1447:1458	arg1	detection					1460:1468	the rapid and quantitative detection	1433:1468	the rapid and quantitative detection of toxic organic pollutants	1433:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	1	55	theme	Ag	220:221	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	6	56	theme	rapid	1437:1441	arg1	detection					1460:1468	the rapid and quantitative detection	1433:1468	the rapid and quantitative detection of toxic organic pollutants	1433:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	1	57	theme	bacterial	246:254	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	6	58	theme	good	1326:1329	arg1	response					1338:1345	The good linear response	1322:1345	The good linear response of the intensity and the logarithmic concentration	1322:1396	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	6	59	theme	promising	1407:1415	arg1	applications					1417:1428	promising applications	1407:1428	promising applications in the rapid and quantitative detection of toxic organic pollutants	1407:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	1	60	theme	synthetic	163:171	arg1	approach					173:180	a well-designed, low-cost, and simple synthetic approach	125:180	a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	125:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	8	61	theme	stable	1685:1690	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	5	62	theme	rhodamine	1104:1112	arg1	thiram					1183:1188	thiram	1183:1188	thiram	1183:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	62	theme	rhodamine	1104:1112	arg1	2-naphthalenethiol					1160:1177	2-naphthalenethiol	1160:1177	2-naphthalenethiol	1160:1177	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	62	theme	rhodamine	1104:1112	arg1	molecules					1093:1101	dye molecules	1089:1101	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	62	theme	rhodamine	1104:1112	arg1	6G					1114:1115	rhodamine 6G	1104:1115	rhodamine 6G	1104:1115	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	2	63	theme	shrinkage	604:612	arg1	treatment					614:622	the controlled silver mirror reaction and volume shrinkage treatment	555:622	the controlled silver mirror reaction and volume shrinkage treatment	555:622	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	3	64	theme	3D	743:744	arg1	spots					750:754	these unique 3D hot spots	730:754	these unique 3D hot spots	730:754	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	6	65	theme	organic	1479:1485	arg1	pollutants					1487:1496	toxic organic pollutants	1473:1496	toxic organic pollutants	1473:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	7	66	theme	detection	1604:1612	arg1	conditions					1614:1623	varied detection conditions	1597:1623	varied detection conditions	1597:1623	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	2	67	theme	BNC	541:543	arg1	fibers					545:550	the networked BNC fibers	527:550	the networked BNC fibers	527:550	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	8	68	theme	BNC	1699:1701	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	4	69	theme	adsorption	924:933	arg1	performances					935:946	adsorption performances	924:946	adsorption performances	924:946	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	2	70	theme	networked	688:696	arg1	substrate					705:713	the 3D networked hybrid substrate	681:713	the 3D networked hybrid substrate	681:713	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	1	71	theme	3D	318:319	arg1	substrate					372:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate	297:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	71	theme	3D	318:319	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	71	theme	3D	318:319	arg1	composites					206:215	the hybrid composites	195:215	the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	195:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	72	theme	SERS	397:400	arg1	sensitivity					402:412	ultrahigh SERS sensitivity	387:412	ultrahigh SERS sensitivity	387:412	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	4	73	theme	hydrophilic	877:887	arg1	BNC					889:891	the hydrophilic BNC	873:891	the hydrophilic BNC	873:891	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	1	74	theme	well-designed	127:139	arg1	approach					173:180	a well-designed, low-cost, and simple synthetic approach	125:180	a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	125:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	7	75	theme	good	1562:1565	arg1	stability					1567:1575	good stability	1562:1575	good stability	1562:1575	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	8	76	theme	@	1698:1698	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	3	77	theme	BNC	781:783	arg1	substrates					785:794	the as-presented Ag-NPs@BNC substrates	757:794	the as-presented Ag-NPs@BNC substrates	757:794	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	0	78	theme	Robust	62:67	arg1	Scattering					92:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering	44:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	1	79	theme	signal	425:430	arg1	reproducibility					432:446	excellent signal reproducibility	415:446	excellent signal reproducibility	415:446	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	8	80	theme	SERS	1744:1747	arg1	substrate					1749:1757	a versatile SERS substrate	1732:1757	a versatile SERS substrate in the rapid identification of various organic molecules	1732:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	8	80	theme	SERS	1744:1747	arg1	potential					1719:1727	potential	1719:1727	potential	1719:1727	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	1	81	theme	low-cost	142:149	arg1	approach					173:180	a well-designed, low-cost, and simple synthetic approach	125:180	a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	125:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	3	82	theme	good	832:835	arg1	reproducibility					846:860	good spectral reproducibility	832:860	good spectral reproducibility	832:860	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	5	83	theme	hybrid	1219:1224	arg1	substrates					1226:1235	the hybrid substrates	1215:1235	the hybrid substrates as SERS substrates	1215:1254	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	3	84	theme	Ag-NPs	774:779	arg1	substrates					785:794	the as-presented Ag-NPs@BNC substrates	757:794	the as-presented Ag-NPs@BNC substrates	757:794	Attributed to these unique 3D hot spots, the as-presented Ag-NPs@BNC substrates exhibited ultrahigh sensitivity and good spectral reproducibility.					
33112614	0	85	theme	Raman	86:90	arg1	Scattering					92:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering	44:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	1	86	theme	flexible	322:329	arg1	substrate					372:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate	297:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	86	theme	flexible	322:329	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	86	theme	flexible	322:329	arg1	composites					206:215	the hybrid composites	195:215	the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	195:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	2	87	dep	created	631:637	arg1	distributed					649:659	distributed	649:659	created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate	631:713	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	7	88	theme	@	1534:1534	arg1	substrate					1539:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	1	89	theme	Raman	348:352	arg1	SERS					366:369	SERS	366:369	SERS	366:369	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	89	theme	Raman	348:352	arg1	scattering					354:363	surface-enhanced Raman scattering	331:363	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	8	90	theme	rapid	1766:1770	arg1	identification					1772:1785	the rapid identification	1762:1785	the rapid identification of various organic molecules	1762:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	7	91	theme	self-supported	1513:1526	arg1	substrate					1539:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	this self-supported Ag-NPs@BNC substrate	1508:1547	Besides, this self-supported Ag-NPs@BNC substrate demonstrated good stability and flexibility for varied detection conditions.					
33112614	1	92	theme	nanoparticle-decorated	223:244	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	5	93	theme	×	1298:1298	arg1	sensitivities					1262:1274	sensitivities	1262:1274	sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M	1262:1305	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	0	94	theme	@	16:16	arg1	Nanocellulose					27:39	@Bacterial Nanocellulose	16:39	@Bacterial Nanocellulose	16:39	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	5	95	theme	dye	1089:1091	arg1	thiram					1183:1188	thiram	1183:1188	thiram	1183:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	95	theme	dye	1089:1091	arg1	6G					1114:1115	rhodamine 6G	1104:1115	rhodamine 6G	1104:1115	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	95	theme	dye	1089:1091	arg1	pollutants					1141:1150	toxic organic pollutants	1127:1150	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	95	theme	dye	1089:1091	arg1	2-naphthalenethiol					1160:1177	2-naphthalenethiol	1160:1177	2-naphthalenethiol	1160:1177	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	95	theme	dye	1089:1091	arg1	molecules					1093:1101	dye molecules	1089:1101	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	95	theme	dye	1089:1091	arg1	result					1072:1077	a result	1070:1077	a result	1070:1077	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	8	96	theme	organic	1798:1804	arg1	molecules					1806:1814	various organic molecules	1790:1814	various organic molecules	1790:1814	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	4	97	theme	spot	1015:1018	arg1	areas					1020:1024	the highly active hot spot areas	993:1024	the highly active hot spot areas	993:1024	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	2	98	theme	hot	667:669	arg1	"					676:676	"hot spots"	666:676	"hot spots" in the 3D networked hybrid substrate	666:713	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	6	99	theme	logarithmic	1372:1382	arg1	concentration					1384:1396	the logarithmic concentration	1368:1396	the logarithmic concentration	1368:1396	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	5	100	theme	×	1283:1283	arg1	10-8					1285:1288	1.6 × 10-8	1279:1288	1.6 × 10-8	1279:1288	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	8	101	theme	3D	1641:1642	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	2	102	theme	homogeneous	468:478	arg1	Ag-NPs					480:485	The homogeneous Ag-NPs	464:485	The homogeneous Ag-NPs with high density	464:503	The homogeneous Ag-NPs with high density were in situ grown on the networked BNC fibers by the controlled silver mirror reaction and volume shrinkage treatment, which created uniformly distributed SERS "hot spots" in the 3D networked hybrid substrate.					
33112614	0	103	theme	Flexible	49:56	arg1	Scattering					92:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering	44:101	a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate	44:111	Ag-Nanoparticles@Bacterial Nanocellulose as a 3D Flexible and Robust Surface-Enhanced Raman Scattering Substrate.					
33112614	1	104	theme	three-dimensional	299:315	arg1	substrate					372:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate	297:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	104	theme	three-dimensional	299:315	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	104	theme	three-dimensional	299:315	arg1	composites					206:215	the hybrid composites	195:215	the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	195:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	4	105	theme	active	1004:1009	arg1	spot					1015:1018	the highly active hot spot	993:1018	the highly active hot spot areas	993:1024	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	5	106	theme	toxic	1127:1131	arg1	thiram					1183:1188	thiram	1183:1188	thiram	1183:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	106	theme	toxic	1127:1131	arg1	pollutants					1141:1150	toxic organic pollutants	1127:1150	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	106	theme	toxic	1127:1131	arg1	2-naphthalenethiol					1160:1177	2-naphthalenethiol	1160:1177	2-naphthalenethiol	1160:1177	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	106	theme	toxic	1127:1131	arg1	molecules					1093:1101	dye molecules	1089:1101	not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram	1080:1188	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	5	106	theme	toxic	1127:1131	arg1	result					1072:1077	a result	1070:1077	a result	1070:1077	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	6	107	from	applications	1417:1428	arg1	detection					1460:1468	the rapid and quantitative detection	1433:1468	the rapid and quantitative detection of toxic organic pollutants	1433:1496	The good linear response of the intensity and the logarithmic concentration revealed promising applications in the rapid and quantitative detection of toxic organic pollutants.					
33112614	5	108	theme	M	1305:1305	arg1	×					1298:1298	3.8 ×	1294:1298	3.8 × 10-9 M	1294:1305	As a result, not only dye molecules (rhodamine 6G) but also toxic organic pollutants such as 2-naphthalenethiol and thiram have been detected using the hybrid substrates as SERS substrates, with sensitivities of 1.6 × 10-8 and 3.8 × 10-9 M, respectively.					
33112614	8	109	theme	networked	1644:1652	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	4	110	from	molecules	980:988	arg1	areas					1020:1024	the highly active hot spot areas	993:1024	the highly active hot spot areas	993:1024	Moreover, the hydrophilic BNC exhibits good permeability and adsorption performances, which could capture the target molecules in the highly active hot spot areas to further improve the SERS sensitivity.					
33112614	8	111	theme	flexible	1655:1662	arg1	substrate					1703:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate	1637:1711	Therefore, the 3D networked, flexible, ultrasensitive, and stable Ag-NPs@BNC substrate shows potential as a versatile SERS substrate in the rapid identification of various organic molecules.					
33112614	1	112	theme	nanocellulose	256:268	arg1	substrate					372:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate	297:380	a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability	297:461	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	112	theme	nanocellulose	256:268	arg1	nanocellulose					256:268	Ag nanoparticle-decorated bacterial nanocellulose	220:268	Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	220:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
33112614	1	112	theme	nanocellulose	256:268	arg1	composites					206:215	the hybrid composites	195:215	the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC)	195:292	We present a well-designed, low-cost, and simple synthetic approach to realizing the hybrid composites of Ag nanoparticle-decorated bacterial nanocellulose (denoted as Ag-NPs@BNC) as a three-dimensional (3D) flexible surface-enhanced Raman scattering (SERS) substrate with ultrahigh SERS sensitivity, excellent signal reproducibility, and stability.					
34878812	6	0	dep	mechanism	804:812	arg1	behind					814:819	behind	814:819	behind their activity	814:834	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	7	1	dep	epimerase	971:979	arg1	the					954:956	the	954:956	the	954:956	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	13	2	theme	valuable	2143:2150	arg1	enzymes					2152:2158	valuable enzymes	2143:2158	valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries	2143:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	13	2	theme	valuable	2143:2150	arg1	epimerases					2117:2126	alginate epimerases	2108:2126	alginate epimerases	2108:2126	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	13	2	theme	valuable	2143:2150	arg1	lyases					2132:2137	lyases	2132:2137	lyases	2132:2137	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	8	3	theme	sequence	1047:1054	arg1	analyses					1056:1063	sequence analyses	1047:1063	sequence analyses	1047:1063	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	10	4	theme	important	1575:1583	arg1	R148					1549:1552	R148	1549:1552	R148	1549:1552	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	4	theme	important	1575:1583	arg1	residue					1585:1591	an important residue	1572:1591	an important residue for the lyase activity	1572:1614	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	6	5	theme	responsible	864:874	arg1	unknown					893:899	unknown	893:899	unknown	893:899	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	6	5	theme	responsible	864:874	arg1	residues					855:862	the amino acid residues	840:862	the amino acid residues responsible for it	840:881	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	7	6	theme	mechanistic	917:927	arg1	determinants					929:940	mechanistic determinants	917:940	mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii	917:1035	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	6	7	theme	acid	850:853	arg1	unknown					893:899	unknown	893:899	unknown	893:899	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	6	7	theme	acid	850:853	arg1	residues					855:862	the amino acid residues	840:862	the amino acid residues responsible for it	840:881	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	1	8	theme	alginates	193:201	arg1	structure					154:162	structure	154:162	structure	154:162	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	1	8	theme	alginates	193:201	arg1	properties					179:188	functional properties	168:188	functional properties	168:188	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	10	9	theme	lyase	1601:1605	arg1	activity					1607:1614	the lyase activity	1597:1614	the lyase activity	1597:1614	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	11	10	theme	reaction	1779:1786	arg1	mechanism					1788:1796	a unified catalytic reaction mechanism	1759:1796	a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid	1759:1920	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	12	11	theme	algal	1976:1980	arg1	constituents					1982:1993	algal constituents	1976:1993	algal constituents	1976:1993	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	8	12	theme	AlgE7	1077:1081	arg1	variants					1083:1090	AlgE7 variants	1077:1090	AlgE7 variants	1077:1090	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	11	13	theme	unified	1761:1767	arg1	mechanism					1788:1796	a unified catalytic reaction mechanism	1759:1796	a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid	1759:1920	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	5	14	theme	mannuronan	750:759	arg1	C-5-epimerases					761:774	mannuronan C-5-epimerases	750:774	mannuronan C-5-epimerases without lyase activity	750:797	These dualistic enzymes share high sequence identity with mannuronan C-5-epimerases without lyase activity.					
34878812	9	15	theme	lyase	1301:1305	arg1	activity					1307:1314	the lyase activity	1297:1314	the lyase activity of AlgE7	1297:1323	Our results show that calcium promotes lyase activity, whereas NaCl reduces the lyase activity of AlgE7.					
34878812	10	16	theme	mutant	1631:1636	arg1	R148G					1638:1642	the point mutant R148G	1621:1642	the point mutant R148G	1621:1642	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	12	17	theme	algal	2076:2080	arg1	biomass					2082:2088	algal biomass	2076:2088	algal biomass	2076:2088	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	5	18	theme	high	722:725	arg1	identity					736:743	high sequence identity	722:743	high sequence identity	722:743	These dualistic enzymes share high sequence identity with mannuronan C-5-epimerases without lyase activity.					
34878812	13	19	theme	food	2289:2292	arg1	industries					2318:2327	food, medicine, and material industries	2289:2327	food, medicine, and material industries	2289:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	13	20	from	applications	2273:2284	arg1	industries					2318:2327	food, medicine, and material industries	2289:2327	food, medicine, and material industries	2289:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	11	21	theme	present	1733:1739	arg1	study					1741:1745	the present study	1729:1745	the present study	1729:1745	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	13	22	theme	medicine	2295:2302	arg1	industries					2318:2327	food, medicine, and material industries	2289:2327	food, medicine, and material industries	2289:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	14	23	theme	polymers	2589:2596	arg1	tailoring					2567:2575	the enzymatic tailoring	2553:2575	the enzymatic tailoring of alginate polymers	2553:2596	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	7	24	from	vinelandii	1026:1035	arg1	epimerase					971:979	bifunctional epimerase	958:979	bifunctional epimerase	958:979	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	7	24	from	vinelandii	1026:1035	arg1	activity					991:998	lyase activity	985:998	lyase activity	985:998	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	12	25	from	strategies	2009:2018	arg1	development					2027:2037	the development	2023:2037	the development of a sustainable bioeconomy based on algal biomass	2023:2088	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	0	26	theme	Azotobacter	76:86	arg1	vinelandii					88:97	the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7	59:147	the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7	59:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	10	27	from	study	1525:1529	arg1	M					1508:1508	M	1508:1508	M	1508:1508	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	12	28	dep	IMPORTANCE	1923:1932	arg1	valorization					1946:1957	Postharvest valorization	1934:1957	Postharvest valorization	1934:1957	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	10	29	theme	AlgE7	1448:1452	arg1	|M||XM|					1471:1474	|M||XM|	1471:1474	|M||XM|	1471:1474	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	29	theme	AlgE7	1448:1452	arg1	AlgE7					1448:1452	AlgE7	1448:1452	AlgE7	1448:1452	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	29	theme	AlgE7	1448:1452	arg1	sites					1439:1443	the preferred cleavage sites	1416:1443	the preferred cleavage sites of AlgE7	1416:1452	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	0	30	theme	Mannuronan	99:108	arg1	C-5-Epimerase					110:122	Mannuronan C-5-Epimerase	99:122	Mannuronan C-5-Epimerase	99:122	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	14	31	from	changes	2445:2451	arg1	conditions					2465:2474	reaction conditions	2456:2474	reaction conditions	2456:2474	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	11	32	theme	Y149	1885:1888	arg1	functions					1890:1898	Y149 functions	1885:1898	Y149 functions	1885:1898	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	11	32	theme	Y149	1885:1888	arg1	H154					1844:1847	H154	1844:1847	H154	1844:1847	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	7	33	from	epimerase	971:979	arg1	vinelandii					1026:1035	Azotobacter vinelandii	1014:1035	Azotobacter vinelandii	1014:1035	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	10	34	theme	polymannuronan	1343:1356	arg1	substrates					1404:1413	defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates	1335:1413	defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates	1335:1413	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	0	35	theme	Mechanistic	0:10	arg1	Basis					12:16	Mechanistic Basis	0:16	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.	0:148	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	13	36	from	enzymes	2152:2158	arg1	respect					2099:2105	this respect	2094:2105	this respect	2094:2105	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	1	37	dep	structure	154:162	arg1	The					150:152	The	150:152	The	150:152	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	10	38	theme	alginate	1386:1393	arg1	substrates					1404:1413	defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates	1335:1413	defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates	1335:1413	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	11	39	theme	catalytic	1907:1915	arg1	H154					1844:1847	H154	1844:1847	H154	1844:1847	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	11	39	theme	catalytic	1907:1915	arg1	acid					1917:1920	the catalytic acid	1903:1920	the catalytic acid	1903:1920	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	8	40	theme	product	1146:1152	arg1	characterization					1154:1169	product characterization	1146:1169	product characterization	1146:1169	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	14	41	theme	lyases	2543:2548	arg1	applications					2502:2513	further applications	2494:2513	further applications of bacterial epimerases and lyases	2494:2548	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	12	42	theme	sustainable	2044:2054	arg1	bioeconomy					2056:2065	a sustainable bioeconomy	2042:2065	a sustainable bioeconomy based on algal biomass	2042:2088	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	4	43	theme	epimerization	600:612	arg1	reactions					624:632	the epimerization and lyase reactions	596:632	the epimerization and lyase reactions	596:632	The reaction mechanisms for the epimerization and lyase reactions are similar, and some enzymes can perform both reactions.					
34878812	3	44	theme	alginate	480:487	arg1	lyases					489:494	alginate lyases	480:494	alginate lyases	480:494	The molecular weight is affected by alginate lyases, which catalyze a β-elimination mechanism that cleaves alginate chains.					
34878812	14	45	theme	epimerases	2528:2537	arg1	applications					2502:2513	further applications	2494:2513	further applications of bacterial epimerases and lyases	2494:2548	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	10	46	theme	mutants	1540:1546	arg1	study					1525:1529	the study	1521:1529	the study of AlgE7 mutants	1521:1546	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	3	47	theme	β-elimination	514:526	arg1	mechanism					528:536	a β-elimination mechanism	512:536	a β-elimination mechanism that cleaves alginate chains	512:565	The molecular weight is affected by alginate lyases, which catalyze a β-elimination mechanism that cleaves alginate chains.					
34878812	8	48	theme	magnetic	1182:1189	arg1	resonance					1191:1199	nuclear magnetic resonance	1174:1199	nuclear magnetic resonance (NMR) spectroscopy	1174:1218	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	8	48	theme	magnetic	1182:1189	arg1	NMR					1202:1204	NMR	1202:1204	NMR	1202:1204	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	4	49	theme	reaction	572:579	arg1	similar					638:644	similar	638:644	similar	638:644	The reaction mechanisms for the epimerization and lyase reactions are similar, and some enzymes can perform both reactions.					
34878812	4	49	theme	reaction	572:579	arg1	mechanisms					581:590	The reaction mechanisms	568:590	The reaction mechanisms for the epimerization and lyase reactions	568:632	The reaction mechanisms for the epimerization and lyase reactions are similar, and some enzymes can perform both reactions.					
34878812	11	50	theme	lyase	1821:1825	arg1	activities					1827:1836	lyase activities	1821:1836	lyase activities	1821:1836	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	14	51	theme	further	2494:2500	arg1	applications					2502:2513	further applications	2494:2513	further applications of bacterial epimerases and lyases	2494:2548	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	10	52	theme	cleavage	1430:1437	arg1	|M||XM|					1471:1474	|M||XM|	1471:1474	|M||XM|	1471:1474	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	52	theme	cleavage	1430:1437	arg1	AlgE7					1448:1452	AlgE7	1448:1452	AlgE7	1448:1452	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	52	theme	cleavage	1430:1437	arg1	sites					1439:1443	the preferred cleavage sites	1416:1443	the preferred cleavage sites of AlgE7	1416:1452	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	3	53	theme	alginate	551:558	arg1	chains					560:565	alginate chains	551:565	alginate chains	551:565	The molecular weight is affected by alginate lyases, which catalyze a β-elimination mechanism that cleaves alginate chains.					
34878812	1	54	theme	weight	257:262	arg1	distribution					264:275	molecular weight distribution	247:275	molecular weight distribution	247:275	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	2	55	theme	residues	397:404	arg1	epimerization					356:368	the epimerization	352:368	the epimerization of β-d-mannuronic acid (M) residues	352:404	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	7	56	theme	bifunctional	958:969	arg1	epimerase					971:979	bifunctional epimerase	958:979	bifunctional epimerase	958:979	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	4	57	theme	lyase	618:622	arg1	reactions					624:632	the epimerization and lyase reactions	596:632	the epimerization and lyase reactions	596:632	The reaction mechanisms for the epimerization and lyase reactions are similar, and some enzymes can perform both reactions.					
34878812	5	58	theme	dualistic	698:706	arg1	enzymes					708:714	These dualistic enzymes	692:714	These dualistic enzymes	692:714	These dualistic enzymes share high sequence identity with mannuronan C-5-epimerases without lyase activity.					
34878812	9	59	theme	lyase	1260:1264	arg1	activity					1266:1273	lyase activity	1260:1273	lyase activity	1260:1273	Our results show that calcium promotes lyase activity, whereas NaCl reduces the lyase activity of AlgE7.					
34878812	12	60	theme	constituents	1982:1993	arg1	upgrading					1963:1971	upgrading	1963:1971	upgrading	1963:1971	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	12	60	theme	constituents	1982:1993	arg1	strategies					2009:2018	promising strategies	1999:2018	promising strategies in the development of a sustainable bioeconomy based on algal biomass	1999:2088	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	12	60	theme	constituents	1982:1993	arg1	IMPORTANCE					1923:1932	IMPORTANCE Postharvest valorization and upgrading	1923:1971	IMPORTANCE	1923:1932	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	10	61	with	enzyme	1659:1664	arg1	activity					1686:1693	only epimerase activity	1671:1693	only epimerase activity	1671:1693	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	13	62	theme	numerous	2264:2271	arg1	applications					2273:2284	numerous applications	2264:2284	numerous applications in food, medicine, and material industries	2264:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	14	63	theme	catalytic	2373:2381	arg1	mechanism					2383:2391	the catalytic mechanism	2369:2391	the catalytic mechanism	2369:2391	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	2	64	theme	monomer	318:324	arg1	composition					326:336	the monomer composition	314:336	the monomer composition	314:336	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	12	65	theme	promising	1999:2007	arg1	upgrading					1963:1971	upgrading	1963:1971	upgrading	1963:1971	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	12	65	theme	promising	1999:2007	arg1	strategies					2009:2018	promising strategies	1999:2018	promising strategies in the development of a sustainable bioeconomy based on algal biomass	1999:2088	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	12	65	theme	promising	1999:2007	arg1	IMPORTANCE					1923:1932	IMPORTANCE Postharvest valorization and upgrading	1923:1971	IMPORTANCE	1923:1932	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	6	66	theme	amino	844:848	arg1	unknown					893:899	unknown	893:899	unknown	893:899	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	6	66	theme	amino	844:848	arg1	residues					855:862	the amino acid residues	840:862	the amino acid residues responsible for it	840:881	The mechanism behind their activity and the amino acid residues responsible for it are still unknown.					
34878812	8	67	theme	variants	1083:1090	arg1	range					1068:1072	a range	1066:1072	a range of AlgE7 variants	1066:1090	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	14	68	theme	better	2345:2350	arg1	understanding					2352:2364	a better understanding	2343:2364	a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions	2343:2474	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	14	69	theme	reaction	2456:2463	arg1	conditions					2465:2474	reaction conditions	2456:2474	reaction conditions	2456:2474	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	10	70	theme	point	1625:1629	arg1	R148G					1638:1642	the point mutant R148G	1621:1642	the point mutant R148G	1621:1642	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	11	71	theme	catalytic	1769:1777	arg1	mechanism					1788:1796	a unified catalytic reaction mechanism	1759:1796	a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid	1759:1920	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	1	72	theme	monomer	223:229	arg1	composition					231:241	the monomer composition	219:241	the monomer composition	219:241	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	14	73	theme	enzymatic	2557:2565	arg1	tailoring					2567:2575	the enzymatic tailoring	2553:2575	the enzymatic tailoring of alginate polymers	2553:2596	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	9	74	theme	AlgE7	1319:1323	arg1	activity					1307:1314	the lyase activity	1297:1314	the lyase activity of AlgE7	1297:1323	Our results show that calcium promotes lyase activity, whereas NaCl reduces the lyase activity of AlgE7.					
34878812	12	75	theme	Postharvest	1934:1944	arg1	valorization					1946:1957	Postharvest valorization	1934:1957	Postharvest valorization	1934:1957	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	5	76	theme	sequence	727:734	arg1	identity					736:743	high sequence identity	722:743	high sequence identity	722:743	These dualistic enzymes share high sequence identity with mannuronan C-5-epimerases without lyase activity.					
34878812	2	77	theme	acid	388:391	arg1	residues					397:404	β-d-mannuronic acid (M) residues	373:404	β-d-mannuronic acid (M) residues	373:404	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	0	78	theme	Bifunctional	63:74	arg1	vinelandii					88:97	the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7	59:147	the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7	59:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	2	79	theme	Mannuronan	278:287	arg1	C-5-epimerases					289:302	Mannuronan C-5-epimerases	278:302	Mannuronan C-5-epimerases	278:302	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	14	80	theme	alginate	2580:2587	arg1	polymers					2589:2596	alginate polymers	2580:2596	alginate polymers	2580:2596	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	10	81	theme	defined	1335:1341	arg1	substrates					1404:1413	defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates	1335:1413	defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates	1335:1413	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	0	82	theme	vinelandii	88:97	arg1	Activities					45:54	the Dual Activities	36:54	the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7	36:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	1	83	theme	functional	168:177	arg1	properties					179:188	functional properties	168:188	functional properties	168:188	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	14	84	theme	mechanism	2383:2391	arg1	understanding					2352:2364	a better understanding	2343:2364	a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions	2343:2474	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	0	85	theme	Lyase	137:141	arg1	AlgE7					143:147	Alginate Lyase AlgE7	128:147	Alginate Lyase AlgE7	128:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	12	86	theme	bioeconomy	2056:2065	arg1	development					2027:2037	the development	2023:2037	the development of a sustainable bioeconomy based on algal biomass	2023:2088	IMPORTANCE Postharvest valorization and upgrading of algal constituents are promising strategies in the development of a sustainable bioeconomy based on algal biomass.					
34878812	13	87	theme	alginate	2108:2115	arg1	enzymes					2152:2158	valuable enzymes	2143:2158	valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries	2143:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	13	87	theme	alginate	2108:2115	arg1	epimerases					2117:2126	alginate epimerases	2108:2126	alginate epimerases	2108:2126	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	13	87	theme	alginate	2108:2115	arg1	lyases					2132:2137	lyases	2132:2137	lyases	2132:2137	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	5	88	theme	lyase	784:788	arg1	activity					790:797	lyase activity	784:797	lyase activity	784:797	These dualistic enzymes share high sequence identity with mannuronan C-5-epimerases without lyase activity.					
34878812	2	89	theme	β-d-mannuronic	373:386	arg1	M					394:394	M	394:394	M	394:394	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	2	89	theme	β-d-mannuronic	373:386	arg1	acid					388:391	β-d-mannuronic acid	373:391	β-d-mannuronic acid (M) residues	373:404	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	0	90	theme	Alginate	128:135	arg1	AlgE7					143:147	Alginate Lyase AlgE7	128:147	Alginate Lyase AlgE7	128:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	8	91	theme	activity	1126:1133	arg1	assays					1135:1140	activity assays	1126:1140	activity assays	1126:1140	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	2	92	theme	G	431:431	arg1	residues					434:441	α-l-guluronic acid (G) residues	411:441	α-l-guluronic acid (G) residues	411:441	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	7	93	theme	Azotobacter	1014:1024	arg1	vinelandii					1026:1035	Azotobacter vinelandii	1014:1035	Azotobacter vinelandii	1014:1035	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	0	94	dep	vinelandii	88:97	arg1	C-5-Epimerase					110:122	Mannuronan C-5-Epimerase	99:122	Mannuronan C-5-Epimerase	99:122	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	0	94	dep	vinelandii	88:97	arg1	AlgE7					143:147	Alginate Lyase AlgE7	128:147	Alginate Lyase AlgE7	128:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	2	95	theme	acid	425:428	arg1	residues					434:441	α-l-guluronic acid (G) residues	411:441	α-l-guluronic acid (G) residues	411:441	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	7	96	theme	AlgE7	1003:1007	arg1	epimerase					971:979	bifunctional epimerase	958:979	bifunctional epimerase	958:979	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	7	96	theme	AlgE7	1003:1007	arg1	activity					991:998	lyase activity	985:998	lyase activity	985:998	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	7	97	from	activity	991:998	arg1	vinelandii					1026:1035	Azotobacter vinelandii	1014:1035	Azotobacter vinelandii	1014:1035	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	10	98	theme	polyalternating	1370:1384	arg1	polyMG					1396:1401	polyMG	1396:1401	polyMG	1396:1401	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	98	theme	polyalternating	1370:1384	arg1	alginate					1386:1393	polyalternating alginate	1370:1393	polyalternating alginate (polyMG)	1370:1402	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	2	99	theme	α-l-guluronic	411:423	arg1	residues					434:441	α-l-guluronic acid (G) residues	411:441	α-l-guluronic acid (G) residues	411:441	Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues.					
34878812	0	100	theme	Dual	40:43	arg1	Activities					45:54	the Dual Activities	36:54	the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7	36:147	Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.					
34878812	8	101	theme	nuclear	1174:1180	arg1	resonance					1191:1199	nuclear magnetic resonance	1174:1199	nuclear magnetic resonance (NMR) spectroscopy	1174:1218	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	8	101	theme	nuclear	1174:1180	arg1	NMR					1202:1204	NMR	1202:1204	NMR	1202:1204	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	13	102	theme	material	2309:2316	arg1	industries					2318:2327	food, medicine, and material industries	2289:2327	food, medicine, and material industries	2289:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	3	103	theme	molecular	448:456	arg1	weight					458:463	The molecular weight	444:463	The molecular weight	444:463	The molecular weight is affected by alginate lyases, which catalyze a β-elimination mechanism that cleaves alginate chains.					
34878812	13	104	theme	functional	2178:2187	arg1	properties					2189:2198	the functional properties	2174:2198	the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries	2174:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	14	105	theme	altered	2434:2440	arg1	understanding					2352:2364	a better understanding	2343:2364	a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions	2343:2474	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	13	106	theme	alginate	2203:2210	arg1	properties					2189:2198	the functional properties	2174:2198	the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries	2174:2327	In this respect, alginate epimerases and lyases are valuable enzymes for tailoring the functional properties of alginate, a polysaccharide extracted from brown seaweed with numerous applications in food, medicine, and material industries.					
34878812	8	107	theme	resonance	1191:1199	arg1	spectroscopy					1207:1218	nuclear magnetic resonance (NMR) spectroscopy	1174:1218	nuclear magnetic resonance (NMR) spectroscopy	1174:1218	Based on sequence analyses, a range of AlgE7 variants were constructed and subjected to activity assays and product characterization by nuclear magnetic resonance (NMR) spectroscopy.					
34878812	14	108	theme	bacterial	2518:2526	arg1	epimerases					2528:2537	bacterial epimerases	2518:2537	bacterial epimerases	2518:2537	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	10	109	theme	AlgE7	1534:1538	arg1	mutants					1540:1546	AlgE7 mutants	1534:1546	AlgE7 mutants	1534:1546	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	7	110	theme	lyase	985:989	arg1	activity					991:998	lyase activity	985:998	lyase activity	985:998	We investigate mechanistic determinants involved in the bifunctional epimerase and lyase activity of AlgE7 from Azotobacter vinelandii.					
34878812	14	111	theme	enzyme	2412:2417	arg1	actions					2419:2425	the two enzyme actions	2404:2425	the two enzyme actions	2404:2425	By providing a better understanding of the catalytic mechanism and of how the two enzyme actions can be altered by changes in reaction conditions, this study opens further applications of bacterial epimerases and lyases in the enzymatic tailoring of alginate polymers.					
34878812	10	112	theme	epimerase	1676:1684	arg1	activity					1686:1693	only epimerase activity	1671:1693	only epimerase activity	1671:1693	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	1	113	theme	molecular	247:255	arg1	distribution					264:275	molecular weight distribution	247:275	molecular weight distribution	247:275	The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution.					
34878812	10	114	theme	preferred	1420:1428	arg1	|M||XM|					1471:1474	|M||XM|	1471:1474	|M||XM|	1471:1474	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	114	theme	preferred	1420:1428	arg1	AlgE7					1448:1452	AlgE7	1448:1452	AlgE7	1448:1452	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	10	114	theme	preferred	1420:1428	arg1	sites					1439:1443	the preferred cleavage sites	1416:1443	the preferred cleavage sites of AlgE7	1416:1452	|By using defined polymannuronan (polyM) and polyalternating alginate (polyMG) substrates, the preferred cleavage sites of AlgE7 were found to be M||XM and G||XM, where X can be either M or G. From the study of AlgE7 mutants, R148 was identified as an important residue for the lyase activity, and the point mutant R148G resulted in an enzyme with only epimerase activity.|					
34878812	11	115	theme	catalytic	1866:1874	arg1	base					1876:1879	the catalytic base	1862:1879	the catalytic base	1862:1879	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
34878812	11	115	theme	catalytic	1866:1874	arg1	H154					1844:1847	H154	1844:1847	H154	1844:1847	Based on the results obtained in the present study, we suggest a unified catalytic reaction mechanism for both epimerase and lyase activities where H154 functions as the catalytic base and Y149 functions as the catalytic acid.					
33671522	7	0	theme	IL-6	1424:1427	arg1	production					1429:1438	IL-6 production	1424:1438	IL-6 production	1424:1438	NGE did not show any effects on inflammatory secretion except inhibited IL-6 production at a high dose.					
33671522	4	1	theme	HPG	865:867	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of TGE, NGE, HPG, and LPG	827:876	The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS).					
33671522	2	2	theme	different	302:310	arg1	NZ					325:326	NZ	325:326	NZ	325:326	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	2	2	theme	different	302:310	arg1	Zealand					316:322	different New Zealand	302:322	different New Zealand (NZ)-grown ginseng fractions	302:351	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	7	3	theme	inflammatory	1384:1395	arg1	secretion					1397:1405	inflammatory secretion	1384:1405	inflammatory secretion	1384:1405	NGE did not show any effects on inflammatory secretion except inhibited IL-6 production at a high dose.					
33671522	4	4	theme	TGE	855:857	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of TGE, NGE, HPG, and LPG	827:876	The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS).					
33671522	4	5	theme	NGE	860:862	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of TGE, NGE, HPG, and LPG	827:876	The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS).					
33671522	3	6	theme	fraction	591:598	arg1	HPG					609:611	HPG	609:611	HPG	609:611	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	6	theme	fraction	591:598	arg1	extract					600:606	high-polar ginsenoside fraction extract	568:606	high-polar ginsenoside fraction extract (HPG)	568:612	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	9	7	theme	µg/mL	1606:1610	arg1	LPG					1612:1614	100 µg/mL LPG	1602:1614	100 µg/mL LPG	1602:1614	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	3	8	theme	high-polar	568:577	arg1	HPG					609:611	HPG	609:611	HPG	609:611	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	8	theme	high-polar	568:577	arg1	extract					600:606	high-polar ginsenoside fraction extract	568:606	high-polar ginsenoside fraction extract (HPG)	568:612	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	1	9	theme	inflammation-related	234:253	arg1	diseases					255:262	inflammation-related diseases	234:262	inflammation-related diseases	234:262	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	3	10	theme	ginsenoside	756:766	arg1	standards					778:786	19 ginsenoside reference standards	753:786	19 ginsenoside reference standards	753:786	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	1	11	theme	Pro-inflammatory	108:123	arg1	cytokines					125:133	Pro-inflammatory cytokines	108:133	Pro-inflammatory cytokines	108:133	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	1	11	theme	Pro-inflammatory	108:123	arg1	cytokines					157:165	anti-inflammatory cytokines	139:165	anti-inflammatory cytokines	139:165	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	1	11	theme	Pro-inflammatory	108:123	arg1	mediators					181:189	important mediators	171:189	important mediators that regulate the inflammatory response in inflammation-related diseases	171:262	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	10	12	theme	non-saponin	1965:1975	arg1	fractions					1977:1985	non-saponin fractions	1965:1985	ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions	1892:1985	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	5	13	theme	interleukin-1	1024:1036	arg1	IL-1β					1044:1048	IL-1β	1044:1048	IL-1β	1044:1048	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	13	theme	interleukin-1	1024:1036	arg1	beta					1038:1041	interleukin-1 beta	1024:1041	interleukin-1 beta (IL-1β)	1024:1049	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	3	14	theme	ginsenoside	630:640	arg1	LPG					660:662	LPG	660:662	LPG	660:662	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	14	theme	ginsenoside	630:640	arg1	extract					651:657	less-polar ginsenoside fraction extract	619:657	less-polar ginsenoside fraction extract (LPG)	619:663	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	6	15	theme	LPS-induced	1312:1322	arg1	productions					1339:1349	LPS-induced TNF-α and IL-6 productions	1312:1349	LPS-induced TNF-α and IL-6 productions	1312:1349	TGE at 400 µg/mL significantly inhibited LPS-induced TNF-α and IL-6 productions.					
33671522	9	16	theme	cytokines	1684:1692	arg1	production					1653:1662	the production	1649:1662	the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6	1649:1715	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	1	17	theme	important	171:179	arg1	cytokines					125:133	Pro-inflammatory cytokines	108:133	Pro-inflammatory cytokines	108:133	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	1	17	theme	important	171:179	arg1	cytokines					157:165	anti-inflammatory cytokines	139:165	anti-inflammatory cytokines	139:165	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	1	17	theme	important	171:179	arg1	mediators					181:189	important mediators	171:189	important mediators that regulate the inflammatory response in inflammation-related diseases	171:262	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	5	18	link	enzyme-linked	1228:1240	arg1	ELISA					1263:1267	ELISA	1263:1267	ELISA	1263:1267	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	18	link	enzyme-linked	1228:1240	arg1	assay					1256:1260	enzyme-linked immunosorbent assay	1228:1260	enzyme-linked immunosorbent assay (ELISA)	1228:1268	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	0	19	theme	Human	79:83	arg1	Cells					101:105	Human Monocytic THP-1 Cells	79:105	Human Monocytic THP-1 Cells	79:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	2	20	theme	THP-1	443:447	arg1	cells					449:453	human monocytic THP-1 cells	427:453	human monocytic THP-1 cells	427:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	3	21	theme	NZ-grown	461:468	arg1	extract					600:606	high-polar ginsenoside fraction extract	568:606	high-polar ginsenoside fraction extract (HPG)	568:612	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	21	theme	NZ-grown	461:468	arg1	extract					553:559	non-ginsenoside fraction extract	528:559	non-ginsenoside fraction extract (NGE)	528:565	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	21	theme	NZ-grown	461:468	arg1	extract					651:657	less-polar ginsenoside fraction extract	619:657	less-polar ginsenoside fraction extract (LPG)	619:663	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	21	theme	NZ-grown	461:468	arg1	fractions					478:486	Four NZ-grown ginseng fractions	456:486	Four NZ-grown ginseng fractions	456:486	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	21	theme	NZ-grown	461:468	arg1	extract					513:519	total ginseng extract	499:519	total ginseng extract (TGE)	499:525	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	9	22	dep	inhibited	1639:1647	arg1	enhanced					1738:1745	enhanced	1738:1745	enhanced the production of anti-inflammatory cytokine IL-10	1738:1796	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	6	23	from	µg/mL	1282:1286	arg1	TGE					1271:1273	TGE	1271:1273	TGE at 400 µg/mL	1271:1286	TGE at 400 µg/mL significantly inhibited LPS-induced TNF-α and IL-6 productions.					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	interleukin-8					1074:1086	interleukin-8	1074:1086	interleukin-8 (IL-8)	1074:1093	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	beta-1					1192:1197	transforming growth factor beta-1	1165:1197	transforming growth factor beta-1 (TGF-β1)	1165:1206	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	cytokines					1118:1126	anti-inflammatory cytokines	1100:1126	anti-inflammatory cytokines	1100:1126	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	interleukin-6					1052:1064	interleukin-6	1052:1064	interleukin-6 (IL-6)	1052:1071	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	beta					1038:1041	interleukin-1 beta	1024:1041	interleukin-1 beta (IL-1β)	1024:1049	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	factor-alpha					1002:1013	tumor necrosis factor-alpha	987:1013	tumor necrosis factor-alpha (TNF-α)	987:1021	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	interleukin-10					1137:1150	interleukin-10	1137:1150	interleukin-10 (IL-10)	1137:1158	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	24	theme	pro-inflammatory	949:964	arg1	cytokines					966:974	pro-inflammatory cytokines	949:974	pro-inflammatory cytokines	949:974	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	0	25	theme	Cytokine	54:61	arg1	Production					63:72	Cytokine Production	54:72	Cytokine Production from Human Monocytic THP-1 Cells	54:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	2	26	theme	anti-inflammatory	396:412	arg1	cytokines					414:422	pro-inflammatory and anti-inflammatory cytokines	375:422	pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells	375:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	5	27	theme	enzyme-linked	1228:1240	arg1	ELISA					1263:1267	ELISA	1263:1267	ELISA	1263:1267	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	27	theme	enzyme-linked	1228:1240	arg1	assay					1256:1260	enzyme-linked immunosorbent assay	1228:1260	enzyme-linked immunosorbent assay (ELISA)	1228:1268	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	2	28	theme	monocytic	433:441	arg1	cells					449:453	human monocytic THP-1 cells	427:453	human monocytic THP-1 cells	427:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	0	29	theme	Zealand	19:25	arg1	Fractions					41:49	New Zealand Grown Ginseng Fractions	15:49	New Zealand Grown Ginseng Fractions	15:49	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	0	30	theme	Ginseng	33:39	arg1	Fractions					41:49	New Zealand Grown Ginseng Fractions	15:49	New Zealand Grown Ginseng Fractions	15:49	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	4	31	theme	THP-1	793:797	arg1	cells					799:803	The THP-1 cells	789:803	The THP-1 cells	789:803	The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS).					
33671522	2	32	theme	ginseng	335:341	arg1	fractions					343:351	different New Zealand (NZ)-grown ginseng fractions	302:351	different New Zealand (NZ)-grown ginseng fractions	302:351	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	9	33	theme	cytokine	1783:1790	arg1	IL-10					1792:1796	anti-inflammatory cytokine IL-10	1765:1796	anti-inflammatory cytokine IL-10	1765:1796	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	2	34	theme	human	427:431	arg1	cells					449:453	human monocytic THP-1 cells	427:453	human monocytic THP-1 cells	427:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	3	35	theme	non-ginsenoside	528:542	arg1	NGE					562:564	NGE	562:564	NGE	562:564	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	35	theme	non-ginsenoside	528:542	arg1	extract					553:559	non-ginsenoside fraction extract	528:559	non-ginsenoside fraction extract (NGE)	528:565	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	5	36	theme	transforming	1165:1176	arg1	TGF-β1					1200:1205	TGF-β1	1200:1205	TGF-β1	1200:1205	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	36	theme	transforming	1165:1176	arg1	beta-1					1192:1197	transforming growth factor beta-1	1165:1197	transforming growth factor beta-1 (TGF-β1)	1165:1206	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	0	37	theme	Fractions	41:49	arg1	Effects					4:10	The Effects	0:10	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells	0:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	5	38	theme	tumor	987:991	arg1	factor-alpha					1002:1013	tumor necrosis factor-alpha	987:1013	tumor necrosis factor-alpha (TNF-α)	987:1021	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	38	theme	tumor	987:991	arg1	TNF-α					1016:1020	TNF-α	1016:1020	TNF-α	1016:1020	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	2	39	from	cells	449:453	arg1	productions					360:370	the productions	356:370	the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells	356:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	8	40	theme	cytokine	1541:1548	arg1	productions					1575:1585	pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions	1524:1585	pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions	1524:1585	Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions.					
33671522	10	41	theme	anti-inflammatory	1826:1842	arg1	effects					1844:1850	anti-inflammatory effects	1826:1850	anti-inflammatory effects	1826:1850	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	2	42	theme	New	312:314	arg1	NZ					325:326	NZ	325:326	NZ	325:326	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	2	42	theme	New	312:314	arg1	Zealand					316:322	different New Zealand	302:322	different New Zealand (NZ)-grown ginseng fractions	302:351	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	2	43	from	productions	360:370	arg1	cells					449:453	human monocytic THP-1 cells	427:453	human monocytic THP-1 cells	427:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	3	44	theme	extracts	716:723	arg1	compositions					700:711	the ginsenoside compositions	684:711	the ginsenoside compositions of extracts	684:723	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	45	theme	ginsenoside	579:589	arg1	HPG					609:611	HPG	609:611	HPG	609:611	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	45	theme	ginsenoside	579:589	arg1	extract					600:606	high-polar ginsenoside fraction extract	568:606	high-polar ginsenoside fraction extract (HPG)	568:612	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	7	46	from	effects	1373:1379	arg1	secretion					1397:1405	inflammatory secretion	1384:1405	inflammatory secretion	1384:1405	NGE did not show any effects on inflammatory secretion except inhibited IL-6 production at a high dose.					
33671522	10	47	theme	ginsenoside	1892:1902	arg1	fractions					1904:1912	ginsenoside fractions	1892:1912	ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions	1892:1985	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	10	47	theme	ginsenoside	1892:1902	arg1	ginsenosides					1939:1950	particularly less-polar ginsenosides	1915:1950	particularly less-polar ginsenosides	1915:1950	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	9	48	dep	cytokines	1684:1692	arg1	cytokines					1684:1692	pro-inflammatory cytokines TNF-α, IL-1β, and IL-6	1667:1715	pro-inflammatory cytokines TNF-α, IL-1β, and IL-6	1667:1715	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	9	48	dep	cytokines	1684:1692	arg1	IL-1β					1701:1705	IL-1β	1701:1705	IL-1β	1701:1705	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	9	48	dep	cytokines	1684:1692	arg1	IL-6					1712:1715	IL-6	1712:1715	IL-6	1712:1715	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	9	48	dep	cytokines	1684:1692	arg1	TNF-α					1694:1698	TNF-α	1694:1698	TNF-α	1694:1698	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	1	49	theme	inflammatory	209:220	arg1	response					222:229	the inflammatory response	205:229	the inflammatory response in inflammation-related diseases	205:262	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	2	50	theme	pro-inflammatory	375:390	arg1	cytokines					414:422	pro-inflammatory and anti-inflammatory cytokines	375:422	pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells	375:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	10	51	theme	less-polar	1928:1937	arg1	fractions					1904:1912	ginsenoside fractions	1892:1912	ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions	1892:1985	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	10	51	theme	less-polar	1928:1937	arg1	ginsenosides					1939:1950	particularly less-polar ginsenosides	1915:1950	particularly less-polar ginsenosides	1915:1950	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	4	52	theme	LPG	874:876	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of TGE, NGE, HPG, and LPG	827:876	The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS).					
33671522	0	53	from	Cells	101:105	arg1	Production					63:72	Cytokine Production	54:72	Cytokine Production from Human Monocytic THP-1 Cells	54:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	3	54	theme	reference	768:776	arg1	standards					778:786	19 ginsenoside reference standards	753:786	19 ginsenoside reference standards	753:786	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	55	theme	less-polar	619:628	arg1	LPG					660:662	LPG	660:662	LPG	660:662	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	55	theme	less-polar	619:628	arg1	extract					651:657	less-polar ginsenoside fraction extract	619:657	less-polar ginsenoside fraction extract (LPG)	619:663	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	1	56	from	response	222:229	arg1	diseases					255:262	inflammation-related diseases	234:262	inflammation-related diseases	234:262	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	3	57	theme	ginseng	505:511	arg1	TGE					522:524	TGE	522:524	TGE	522:524	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	57	theme	ginseng	505:511	arg1	extract					513:519	total ginseng extract	499:519	total ginseng extract (TGE)	499:525	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	6	58	theme	TNF-α	1324:1328	arg1	productions					1339:1349	LPS-induced TNF-α and IL-6 productions	1312:1349	LPS-induced TNF-α and IL-6 productions	1312:1349	TGE at 400 µg/mL significantly inhibited LPS-induced TNF-α and IL-6 productions.					
33671522	1	59	theme	anti-inflammatory	139:155	arg1	cytokines					125:133	Pro-inflammatory cytokines	108:133	Pro-inflammatory cytokines	108:133	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	1	59	theme	anti-inflammatory	139:155	arg1	cytokines					157:165	anti-inflammatory cytokines	139:165	anti-inflammatory cytokines	139:165	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	1	59	theme	anti-inflammatory	139:155	arg1	mediators					181:189	important mediators	171:189	important mediators that regulate the inflammatory response in inflammation-related diseases	171:262	Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases.					
33671522	9	60	theme	pro-inflammatory	1667:1682	arg1	cytokines					1684:1692	pro-inflammatory cytokines TNF-α, IL-1β, and IL-6	1667:1715	pro-inflammatory cytokines TNF-α, IL-1β, and IL-6	1667:1715	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	9	60	theme	pro-inflammatory	1667:1682	arg1	IL-1β					1701:1705	IL-1β	1701:1705	IL-1β	1701:1705	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	9	60	theme	pro-inflammatory	1667:1682	arg1	IL-6					1712:1715	IL-6	1712:1715	IL-6	1712:1715	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	9	60	theme	pro-inflammatory	1667:1682	arg1	TNF-α					1694:1698	TNF-α	1694:1698	TNF-α	1694:1698	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	5	61	theme	factor	1185:1190	arg1	TGF-β1					1200:1205	TGF-β1	1200:1205	TGF-β1	1200:1205	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	61	theme	factor	1185:1190	arg1	beta-1					1192:1197	transforming growth factor beta-1	1165:1197	transforming growth factor beta-1 (TGF-β1)	1165:1206	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	2	62	theme	study	281:285	arg1	aim					269:271	The aim	265:271	The aim of this study	265:285	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	0	63	from	Effects	4:10	arg1	Production					63:72	Cytokine Production	54:72	Cytokine Production from Human Monocytic THP-1 Cells	54:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	0	64	theme	Monocytic	85:93	arg1	Cells					101:105	Human Monocytic THP-1 Cells	79:105	Human Monocytic THP-1 Cells	79:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	2	65	theme	Zealand	316:322	arg1	fractions					343:351	different New Zealand (NZ)-grown ginseng fractions	302:351	different New Zealand (NZ)-grown ginseng fractions	302:351	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	8	66	dep	cytokine	1541:1548	arg1	IL-1β					1558:1562	IL-1β	1558:1562	IL-1β	1558:1562	Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions.					
33671522	8	66	dep	cytokine	1541:1548	arg1	IL-6					1569:1572	IL-6	1569:1572	IL-6	1569:1572	Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions.					
33671522	8	66	dep	cytokine	1541:1548	arg1	TNF-α					1551:1555	TNF-α	1551:1555	TNF-α	1551:1555	Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions.					
33671522	2	67	theme	cytokines	414:422	arg1	productions					360:370	the productions	356:370	the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells	356:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	0	68	theme	New	15:17	arg1	Zealand					19:25	New Zealand	15:25	New Zealand Grown Ginseng Fractions	15:49	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	8	69	theme	stronger	1485:1492	arg1	effect					1494:1499	a stronger effect	1483:1499	a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions	1483:1585	Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions.					
33671522	5	70	theme	immunosorbent	1242:1254	arg1	ELISA					1263:1267	ELISA	1263:1267	ELISA	1263:1267	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	70	theme	immunosorbent	1242:1254	arg1	assay					1256:1260	enzyme-linked immunosorbent assay	1228:1260	enzyme-linked immunosorbent assay (ELISA)	1228:1268	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	3	71	theme	total	499:503	arg1	TGE					522:524	TGE	522:524	TGE	522:524	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	71	theme	total	499:503	arg1	extract					513:519	total ginseng extract	499:519	total ginseng extract (TGE)	499:525	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	0	72	theme	Grown	27:31	arg1	Fractions					41:49	New Zealand Grown Ginseng Fractions	15:49	New Zealand Grown Ginseng Fractions	15:49	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	5	73	theme	necrosis	993:1000	arg1	factor-alpha					1002:1013	tumor necrosis factor-alpha	987:1013	tumor necrosis factor-alpha (TNF-α)	987:1021	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	73	theme	necrosis	993:1000	arg1	TNF-α					1016:1020	TNF-α	1016:1020	TNF-α	1016:1020	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	3	74	theme	fraction	642:649	arg1	LPG					660:662	LPG	660:662	LPG	660:662	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	74	theme	fraction	642:649	arg1	extract					651:657	less-polar ginsenoside fraction extract	619:657	less-polar ginsenoside fraction extract (LPG)	619:663	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	0	75	theme	THP-1	95:99	arg1	Cells					101:105	Human Monocytic THP-1 Cells	79:105	Human Monocytic THP-1 Cells	79:105	The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.					
33671522	6	76	theme	IL-6	1334:1337	arg1	productions					1339:1349	LPS-induced TNF-α and IL-6 productions	1312:1349	LPS-induced TNF-α and IL-6 productions	1312:1349	TGE at 400 µg/mL significantly inhibited LPS-induced TNF-α and IL-6 productions.					
33671522	4	77	theme	different	827:835	arg1	concentrations					837:850	different concentrations	827:850	different concentrations of TGE, NGE, HPG, and LPG	827:876	The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS).					
33671522	5	78	theme	anti-inflammatory	1100:1116	arg1	cytokines					1118:1126	anti-inflammatory cytokines	1100:1126	anti-inflammatory cytokines	1100:1126	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	7	79	theme	high	1445:1448	arg1	dose					1450:1453	a high dose	1443:1453	a high dose	1443:1453	NGE did not show any effects on inflammatory secretion except inhibited IL-6 production at a high dose.					
33671522	5	80	theme	cytokines	966:974	arg1	levels					939:944	The levels	935:944	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1),	935:1207	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	2	81	from	cytokines	414:422	arg1	cells					449:453	human monocytic THP-1 cells	427:453	human monocytic THP-1 cells	427:453	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	9	82	theme	anti-inflammatory	1765:1781	arg1	IL-10					1792:1796	anti-inflammatory cytokine IL-10	1765:1796	anti-inflammatory cytokine IL-10	1765:1796	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	8	83	theme	pro-inflammatory	1524:1539	arg1	cytokine					1541:1548	pro-inflammatory cytokine	1524:1548	pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions	1524:1585	Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions.					
33671522	10	84	theme	NZ-grown	1799:1806	arg1	ginseng					1808:1814	NZ-grown ginseng	1799:1814	NZ-grown ginseng	1799:1814	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	2	85	theme	-grown	328:333	arg1	fractions					343:351	different New Zealand (NZ)-grown ginseng fractions	302:351	different New Zealand (NZ)-grown ginseng fractions	302:351	The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells.					
33671522	3	86	theme	ginseng	470:476	arg1	extract					600:606	high-polar ginsenoside fraction extract	568:606	high-polar ginsenoside fraction extract (HPG)	568:612	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	86	theme	ginseng	470:476	arg1	extract					553:559	non-ginsenoside fraction extract	528:559	non-ginsenoside fraction extract (NGE)	528:565	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	86	theme	ginseng	470:476	arg1	extract					651:657	less-polar ginsenoside fraction extract	619:657	less-polar ginsenoside fraction extract (LPG)	619:663	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	86	theme	ginseng	470:476	arg1	fractions					478:486	Four NZ-grown ginseng fractions	456:486	Four NZ-grown ginseng fractions	456:486	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	86	theme	ginseng	470:476	arg1	extract					513:519	total ginseng extract	499:519	total ginseng extract (TGE)	499:525	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	9	87	theme	IL-10	1792:1796	arg1	production					1751:1760	the production	1747:1760	the production of anti-inflammatory cytokine IL-10	1747:1796	Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10.					
33671522	10	88	dep	exhibited	1816:1824	arg1	attributed					1878:1887	attributed	1878:1887	is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions	1868:1985	NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.					
33671522	3	89	theme	ginsenoside	688:698	arg1	compositions					700:711	the ginsenoside compositions	684:711	the ginsenoside compositions of extracts	684:723	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	90	theme	fraction	544:551	arg1	NGE					562:564	NGE	562:564	NGE	562:564	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	3	90	theme	fraction	544:551	arg1	extract					553:559	non-ginsenoside fraction extract	528:559	non-ginsenoside fraction extract (NGE)	528:565	Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards.					
33671522	5	91	theme	growth	1178:1183	arg1	TGF-β1					1200:1205	TGF-β1	1200:1205	TGF-β1	1200:1205	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33671522	5	91	theme	growth	1178:1183	arg1	beta-1					1192:1197	transforming growth factor beta-1	1165:1197	transforming growth factor beta-1 (TGF-β1)	1165:1206	The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA).					
33862078	8	0	theme	antimicrobial	1531:1543	arg1	activity					1545:1552	The antimicrobial activity	1527:1552	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli)	1527:1668	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli) was investigated.					
33862078	6	1	theme	irradiated	1214:1223	arg1	%					1201:1201	80/20%	1196:1201	80/20%	1196:1201	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	6	1	theme	irradiated	1214:1223	arg1	PVA/PLST					1186:1193	PVA/PLST	1186:1193	PVA/PLST (80/20%)	1186:1202	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	7	2	theme	irradiation	1388:1398	arg1	dose					1400:1403	the irradiation dose	1384:1403	the irradiation dose	1384:1403	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	6	3	theme	CRA	1148:1150	arg1	value					1153:1157	crease recovery angle (CRA) value	1125:1157	crease recovery angle (CRA) value of 147.6	1125:1166	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	4	4	theme	mechanical	793:802	arg1	properties					842:851	tensile mechanical, crease recovery and water absorption properties	785:851	properties	842:851	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	4	theme	mechanical	793:802	arg1	recovery					812:819	crease recovery	805:819	crease recovery	805:819	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	4	theme	mechanical	793:802	arg1	absorption					831:840	water absorption	825:840	water absorption	825:840	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	6	5	theme	angle	1141:1145	arg1	value					1153:1157	crease recovery angle (CRA) value	1125:1157	crease recovery angle (CRA) value of 147.6	1125:1166	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	3	6	theme	cotton/polyester	528:543	arg1	fabrics					545:551	Cotton and cotton/polyester fabrics	517:551	fabrics	545:551	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	1	7	theme	copper/chitosan	195:209	arg1	nanocomposites					211:224	copper/chitosan nanocomposites	195:224	copper/chitosan nanocomposites (Cu/CS)	195:232	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	1	7	theme	copper/chitosan	195:209	arg1	stabilizer					296:305	stabilizer	296:305	stabilizer	296:305	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	1	7	theme	copper/chitosan	195:209	arg1	CuSO4·5H2O					311:320	CuSO4·5H2O	311:320	CuSO4·5H2O	311:320	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	1	7	theme	copper/chitosan	195:209	arg1	Cu/CS					227:231	Cu/CS	227:231	Cu/CS	227:231	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	4	8	theme	crease	805:810	arg1	properties					842:851	tensile mechanical, crease recovery and water absorption properties	785:851	properties	842:851	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	8	theme	crease	805:810	arg1	recovery					812:819	crease recovery	805:819	crease recovery	805:819	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	7	9	theme	cotton	1324:1329	arg1	fabrics					1331:1337	cotton fabrics	1324:1337	cotton fabrics	1324:1337	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	9	10	theme	zone	1826:1829	arg1	50/50					1772:1776	50/50	1772:1776	50/50	1772:1776	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	9	10	theme	zone	1826:1829	arg1	PVA/PLST					1788:1795	20/80 PVA/PLST	1782:1795	20/80 PVA/PLST of 14 and 14.5 mm inhibition zone	1782:1829	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	3	11	theme	plasticized	707:717	arg1	PLST					727:730	PLST	727:730	PLST	727:730	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	3	11	theme	plasticized	707:717	arg1	starch					719:724	plasticized starch	707:724	plasticized starch (PLST)	707:731	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	7	12	with	strength	1427:1434	arg1	formulations					1477:1488	the different formulations	1463:1488	the different formulations	1463:1488	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	9	13	theme	cotton/polyester	1840:1855	arg1	fabric					1857:1862	cotton/polyester fabric	1840:1862	cotton/polyester fabric	1840:1862	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	6	14	theme	CRA	1247:1249	arg1	value					1251:1255	CRA value	1247:1255	CRA value of 125.0 for cotton fabrics treated under the same conditions	1247:1317	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	1	15	from	solution	262:269	arg1	presence					278:285	the presence	274:285	the presence of CS	274:291	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	7	16	theme	untreated	1509:1517	arg1	fabric					1519:1524	the untreated fabric	1505:1524	the untreated fabric	1505:1524	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	6	17	theme	147.6	1162:1166	arg1	value					1153:1157	crease recovery angle (CRA) value	1125:1157	crease recovery angle (CRA) value of 147.6	1125:1166	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	8	18	dep	bacteria	1591:1598	arg1	aurous					1616:1621	Staphylococcus aurous	1601:1621	Staphylococcus aurous	1601:1621	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli) was investigated.					
33862078	7	19	theme	tensile	1344:1350	arg1	strength					1352:1359	the tensile strength	1340:1359	the tensile strength	1340:1359	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	9	20	theme	mm	1881:1882	arg1	zone					1895:1898	6 and 5 mm inhibition zone	1873:1898	6 and 5 mm inhibition zone against gram-negative bacteria	1873:1929	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	6	21	theme	same	1303:1306	arg1	conditions					1308:1317	the same conditions	1299:1317	the same conditions	1299:1317	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	4	22	dep	properties	842:851	arg1	properties					842:851	tensile mechanical, crease recovery and water absorption properties	785:851	properties	842:851	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	22	dep	properties	842:851	arg1	recovery					812:819	crease recovery	805:819	crease recovery	805:819	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	22	dep	properties	842:851	arg1	absorption					831:840	water absorption	825:840	water absorption	825:840	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	2	23	dep	Fourier	448:454	arg1	Transform					456:464	Transform	456:464	Transform infrared (FT-IR) spectroscopy	456:494	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	7	24	theme	different	1467:1475	arg1	formulations					1477:1488	the different formulations	1463:1488	the different formulations	1463:1488	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	9	25	theme	gram-negative	1908:1920	arg1	bacteria					1922:1929	gram-negative bacteria	1908:1929	gram-negative bacteria	1908:1929	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	9	26	theme	gram-positive	1699:1711	arg1	bacteria					1713:1720	gram-positive bacteria	1699:1720	gram-positive bacteria	1699:1720	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	0	27	theme	polymer	136:142	arg1	blends					144:149	loaded polymer blends	129:149	loaded polymer blends	129:149	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	5	28	theme	PVA	1063:1065	arg1	ratio					1054:1058	the ratio	1050:1058	the ratio of PVA	1050:1065	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	9	29	theme	cotton	1722:1727	arg1	fabric					1729:1734	cotton fabric	1722:1734	cotton fabric	1722:1734	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	0	30	theme	Antibacterial	0:12	arg1	functionalization					14:30	Antibacterial functionalization	0:30	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan	0:112	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	1	31	theme	CS	290:291	arg1	presence					278:285	the presence	274:285	the presence of CS	274:291	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	5	32	theme	higher	903:908	arg1	%					928:928	%	928:928	%	928:928	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	5	32	theme	higher	903:908	arg1	absorption					916:925	higher water absorption	903:925	higher water absorption (%)	903:929	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	9	33	theme	highest	1747:1753	arg1	impact					1755:1760	the highest impact	1743:1760	the highest impact	1743:1760	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	2	34	theme	Dynamic	416:422	arg1	DLS					442:444	DLS	442:444	DLS	442:444	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	2	34	theme	Dynamic	416:422	arg1	scattering					430:439	Dynamic light scattering	416:439	Dynamic light scattering (DLS)	416:445	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	0	35	theme	hybrid	80:85	arg1	coating					87:93	hybrid coating	80:93	hybrid coating of copper/chitosan	80:112	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	0	36	theme	fabrics	63:69	arg1	functionalization					14:30	Antibacterial functionalization	0:30	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan	0:112	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	5	37	theme	water	995:999	arg1	absorption					1001:1010	water absorption	995:1010	water absorption	995:1010	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	4	38	theme	grafted	738:744	arg1	fabrics					746:752	The grafted fabrics	734:752	The grafted fabrics	734:752	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	2	39	theme	NPs	343:345	arg1	formation					347:355	The Cu/CS NPs formation	333:355	The Cu/CS NPs formation	333:355	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	2	40	theme	electron	389:396	arg1	TEM					410:412	TEM	410:412	TEM	410:412	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	2	40	theme	electron	389:396	arg1	microscopy					398:407	transmission electron microscopy	376:407	transmission electron microscopy (TEM)	376:413	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	1	41	theme	aqueous	254:260	arg1	solution					262:269	an aqueous solution	251:269	an aqueous solution in the presence of CS	251:291	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	3	42	theme	vinyl	682:686	arg1	poly					677:680	poly	677:680	poly(vinyl alcohol) (PVA)	677:701	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	3	42	theme	vinyl	682:686	arg1	alcohol					688:694	vinyl alcohol	682:694	vinyl alcohol	682:694	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	3	43	theme	poly	677:680	arg1	blends					667:672	polymer blends	659:672	polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST)	659:731	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	6	44	theme	crease	1125:1130	arg1	value					1153:1157	crease recovery angle (CRA) value	1125:1157	crease recovery angle (CRA) value of 147.6	1125:1166	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	4	45	theme	water	825:829	arg1	properties					842:851	tensile mechanical, crease recovery and water absorption properties	785:851	properties	842:851	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	45	theme	water	825:829	arg1	absorption					831:840	water absorption	825:840	water absorption	825:840	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	46	theme	tensile	785:791	arg1	properties					842:851	tensile mechanical, crease recovery and water absorption properties	785:851	properties	842:851	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	46	theme	tensile	785:791	arg1	recovery					812:819	crease recovery	805:819	crease recovery	805:819	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	4	46	theme	tensile	785:791	arg1	absorption					831:840	water absorption	825:840	water absorption	825:840	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	6	47	theme	recovery	1132:1139	arg1	value					1153:1157	crease recovery angle (CRA) value	1125:1157	crease recovery angle (CRA) value of 147.6	1125:1166	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	9	48	theme	inhibition	1815:1824	arg1	zone					1826:1829	14 and 14.5 mm inhibition zone	1800:1829	14 and 14.5 mm inhibition zone	1800:1829	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	8	49	theme	gram-negative	1628:1640	arg1	bacteria					1642:1649	gram-negative bacteria	1628:1649	gram-negative bacteria (Escherichia coli)	1628:1668	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli) was investigated.					
33862078	3	50	theme	starch	719:724	arg1	blends					667:672	polymer blends	659:672	polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST)	659:731	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	6	51	theme	125.0	1260:1264	arg1	value					1251:1255	CRA value	1247:1255	CRA value of 125.0 for cotton fabrics treated under the same conditions	1247:1317	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	6	52	theme	Cotton/polyester	1091:1106	arg1	fabrics					1108:1114	Cotton/polyester fabrics	1091:1114	Cotton/polyester fabrics	1091:1114	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	9	53	dep	zone	1826:1829	arg1	mm					1812:1813	mm	1812:1813	mm	1812:1813	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	0	54	theme	gamma	155:159	arg1	irradiation					161:171	gamma irradiation	155:171	gamma irradiation	155:171	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	4	55	theme	properties	842:851	arg1	terms					776:780	terms	776:780	terms of tensile mechanical, crease recovery and water absorption properties	776:851	The grafted fabrics were characterized in terms of tensile mechanical, crease recovery and water absorption properties.					
33862078	9	56	theme	6	1873:1873	arg1	mm					1881:1882	mm	1881:1882	mm	1881:1882	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	3	57	from	gamma-radiation	558:572	arg1	nanocomposites					615:628	nanocomposites	615:628	nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST)	615:731	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	9	58	dep	mm	1812:1813	arg1	14.5					1807:1810	14.5	1807:1810	14.5	1807:1810	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	9	58	dep	mm	1812:1813	arg1	14					1800:1801	14	1800:1801	14	1800:1801	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	5	59	theme	cotton	878:883	arg1	fabrics					885:891	cotton fabrics	878:891	cotton fabrics	878:891	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	9	60	theme	5	1879:1879	arg1	zone					1895:1898	6 and 5 mm inhibition zone	1873:1898	6 and 5 mm inhibition zone against gram-negative bacteria	1873:1929	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	3	61	theme	%	609:609	arg1	pickup					596:601	pickup	596:601	pickup of 100%	596:609	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	1	62	dep	stabilizer	296:305	arg1	precursor					322:330	precursor	322:330	precursor	322:330	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	9	63	theme	inhibition	1884:1893	arg1	zone					1895:1898	6 and 5 mm inhibition zone	1873:1898	6 and 5 mm inhibition zone against gram-negative bacteria	1873:1929	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	0	64	theme	copper/chitosan	98:112	arg1	coating					87:93	hybrid coating	80:93	hybrid coating of copper/chitosan	80:112	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	5	65	theme	PVA/PLST	1074:1081	arg1	blends					1083:1088	the PVA/PLST blends	1070:1088	the PVA/PLST blends	1070:1088	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	1	66	theme	present	181:187	arg1	work					189:192	the present work	177:192	the present work	177:192	In the present work, copper/chitosan nanocomposites (Cu/CS) were prepared in an aqueous solution in the presence of CS as stabilizer and CuSO4·5H2O precursor.					
33862078	2	67	theme	FT-IR	476:480	arg1	spectroscopy					483:494	infrared (FT-IR) spectroscopy	466:494	infrared (FT-IR) spectroscopy	466:494	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	0	68	theme	loaded	129:134	arg1	blends					144:149	loaded polymer blends	129:149	loaded polymer blends	129:149	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	7	69	theme	fabric	1451:1456	arg1	higher					1493:1498	higher	1493:1498	higher	1493:1498	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	7	69	theme	fabric	1451:1456	arg1	strength					1427:1434	the tensile strength	1415:1434	the tensile strength of the treated fabric with the different formulations	1415:1488	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	6	70	theme	cotton	1270:1275	arg1	fabrics					1277:1283	cotton fabrics	1270:1283	cotton fabrics treated under the same conditions	1270:1317	Cotton/polyester fabrics displays crease recovery angle (CRA) value of 147.6 upon treated with PVA/PLST (80/20%) and gamma irradiated to 30 kGy compared to CRA value of 125.0 for cotton fabrics treated under the same conditions.					
33862078	2	71	theme	XRD	500:502	arg1	diffraction					504:514	XRD diffraction	500:514	XRD diffraction	500:514	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	8	72	dep	bacteria	1642:1649	arg1	coli					1664:1667	Escherichia coli	1652:1667	Escherichia coli	1652:1667	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli) was investigated.					
33862078	9	73	theme	20/80	1945:1949	arg1	PVA/PLST					1951:1958	20/80 PVA/PLST	1945:1958	20/80 PVA/PLST	1945:1958	In case of gram-positive bacteria cotton fabric showed the highest impact, for both 50/50 and 20/80 PVA/PLST of 14 and 14.5 mm inhibition zone, whilst, cotton/polyester fabric recorded 6 and 5 mm inhibition zone against gram-negative bacteria for 50/50 and 20/80 PVA/PLST, respectively.					
33862078	0	74	theme	cotton	35:40	arg1	fabrics					63:69	cotton and cotton/polyester fabrics	35:69	cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan	35:112	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	5	75	theme	water	910:914	arg1	%					928:928	%	928:928	%	928:928	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	5	75	theme	water	910:914	arg1	absorption					916:925	higher water absorption	903:925	higher water absorption (%)	903:929	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	3	76	theme	Cu/CS	639:643	arg1	NPs					645:647	Cu/CS NPs	639:647	Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST)	639:731	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	2	77	theme	light	424:428	arg1	DLS					442:444	DLS	442:444	DLS	442:444	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	2	77	theme	light	424:428	arg1	scattering					430:439	Dynamic light scattering	416:439	Dynamic light scattering (DLS)	416:445	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	0	78	theme	cotton/polyester	46:61	arg1	fabrics					63:69	cotton and cotton/polyester fabrics	35:69	cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan	35:112	Antibacterial functionalization of cotton and cotton/polyester fabrics applying hybrid coating of copper/chitosan nanocomposites loaded polymer blends via gamma irradiation.					
33862078	7	79	theme	treated	1443:1449	arg1	fabric					1451:1456	the treated fabric	1439:1456	the treated fabric	1439:1456	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	5	80	theme	PVA/PLST	969:976	arg1	compositions					978:989	all PVA/PLST compositions	965:989	all PVA/PLST compositions	965:989	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	2	81	theme	infrared	466:473	arg1	spectroscopy					483:494	infrared (FT-IR) spectroscopy	466:494	infrared (FT-IR) spectroscopy	466:494	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	2	82	theme	transmission	376:387	arg1	TEM					410:412	TEM	410:412	TEM	410:412	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	2	82	theme	transmission	376:387	arg1	microscopy					398:407	transmission electron microscopy	376:407	transmission electron microscopy (TEM)	376:413	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	3	83	theme	polymer	659:665	arg1	blends					667:672	polymer blends	659:672	polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST)	659:731	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
33862078	8	84	theme	fabrics	1561:1567	arg1	activity					1545:1552	The antimicrobial activity	1527:1552	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli)	1527:1668	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli) was investigated.					
33862078	7	85	theme	tensile	1419:1425	arg1	higher					1493:1498	higher	1493:1498	higher	1493:1498	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	7	85	theme	tensile	1419:1425	arg1	strength					1427:1434	the tensile strength	1415:1434	the tensile strength of the treated fabric with the different formulations	1415:1488	For cotton fabrics, the tensile strength was largely depends on the irradiation dose, in which the tensile strength of the treated fabric with the different formulations is higher than the untreated fabric.					
33862078	5	86	theme	cotton/polyester	936:951	arg1	fabrics					953:959	cotton/polyester fabrics	936:959	cotton/polyester fabrics for all PVA/PLST compositions	936:989	The results showed that cotton fabrics displayed higher water absorption (%) than cotton/polyester fabrics for all PVA/PLST compositions and water absorption was found to decrease with increasing the ratio of PVA in the PVA/PLST blends.					
33862078	2	87	theme	Cu/CS	337:341	arg1	formation					347:355	The Cu/CS NPs formation	333:355	The Cu/CS NPs formation	333:355	The Cu/CS NPs formation was proved through transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform infrared (FT-IR) spectroscopy and XRD diffraction.					
33862078	8	88	theme	gram-positive	1577:1589	arg1	bacteria					1591:1598	gram-positive bacteria	1577:1598	gram-positive bacteria (Staphylococcus aurous)	1577:1622	The antimicrobial activity of the fabrics against gram-positive bacteria (Staphylococcus aurous) and gram-negative bacteria (Escherichia coli) was investigated.					
33862078	3	89	theme	Cotton	517:522	arg1	fabrics					545:551	Cotton and cotton/polyester fabrics	517:551	fabrics	545:551	Cotton and cotton/polyester fabrics were gamma-radiation grafted by padding to pickup of 100%, in nanocomposites based on Cu/CS NPs loaded in polymer blends of poly(vinyl alcohol) (PVA) and plasticized starch (PLST).					
32144305	2	0	from	faeces	409:414	arg1	infants					477:483	healthy, breastfed infants	458:483	healthy, breastfed infants	458:483	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	4	1	theme	delivery	802:809	arg1	mode					794:797	mode	794:797	mode of delivery and breastmilk LNFP III concentration at two weeks	794:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	2	from	composition	443:453	arg1	infants					477:483	healthy, breastfed infants	458:483	healthy, breastfed infants	458:483	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	2	3	theme	selected	343:350	arg1	oligosaccharides					363:378	selected human milk oligosaccharides	343:378	selected human milk oligosaccharides	343:378	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	0	4	theme	breastfed	85:93	arg1	infants					95:101	healthy breastfed infants	77:101	healthy breastfed infants at two, six, and twelve weeks of age	77:138	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	0	5	from	association	4:14	arg1	weeks					127:131	two, six, and twelve weeks	106:131	two, six, and twelve weeks of age	106:138	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	0	5	from	association	4:14	arg1	infants					95:101	healthy breastfed infants	77:101	healthy breastfed infants at two, six, and twelve weeks of age	77:138	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	1	6	theme	Several	141:147	arg1	factors					149:155	Several factors	141:155	Several factors	141:155	Several factors affect gut microbiota development in early life, among which breastfeeding plays a key role.					
32144305	5	7	theme	OTUs	1115:1118	arg1	abundance					1102:1110	relative abundance	1093:1110	relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs,	1093:1195	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	5	7	theme	OTUs	1115:1118	arg1	levels					1052:1057	levels	1052:1057	levels of individual breastmilk HMOs	1052:1087	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	3	8	contain	had	537:539	arg2	effect					555:560	a significant effect	541:560	a significant effect	541:560	Lactation duration had a significant effect on breastmilk HMO content, which decreased with time, except for 3-fucosyllactose (3FL) and Lacto-N-fucopentaose III (LNFP III).					
32144305	3	8	contain	had	537:539	arg1	duration					528:535	Lactation duration	518:535	Lactation duration	518:535	Lactation duration had a significant effect on breastmilk HMO content, which decreased with time, except for 3-fucosyllactose (3FL) and Lacto-N-fucopentaose III (LNFP III).					
32144305	4	9	theme	breastmilk	815:824	arg1	concentration					835:847	breastmilk LNFP III concentration	815:847	breastmilk LNFP III concentration at two weeks	815:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	1	10	theme	key	240:242	arg1	role					244:247	a key role	238:247	a key role	238:247	Several factors affect gut microbiota development in early life, among which breastfeeding plays a key role.					
32144305	4	11	theme	infant	868:873	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	12	from	mode	794:797	arg1	weeks					856:860	two weeks	852:860	two weeks	852:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	5	13	theme	relative	1093:1100	arg1	abundance					1102:1110	relative abundance	1093:1110	relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs,	1093:1195	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	4	14	theme	concentration	835:847	arg1	mode					794:797	mode	794:797	mode of delivery and breastmilk LNFP III concentration at two weeks	794:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	5	15	theme	individual	1062:1071	arg1	HMOs					1084:1087	individual breastmilk HMOs	1062:1087	individual breastmilk HMOs	1062:1087	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	2	16	theme	two	488:490	arg1	weeks					504:508	two, six and 12 weeks	488:508	weeks	504:508	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	5	17	theme	Bifidobacterium	1175:1189	arg1	OTUs					1191:1194	the most predominant Bifidobacterium OTUs	1154:1194	the most predominant Bifidobacterium OTUs	1154:1194	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	4	18	theme	microbiota	709:718	arg1	composition					720:730	microbiota composition	709:730	microbiota composition	709:730	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	19	theme	faecal	425:430	arg1	composition					443:453	the faecal microbiota composition	421:453	the faecal microbiota composition in healthy, breastfed infants	421:483	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	5	20	theme	HMOs	1084:1087	arg1	abundance					1102:1110	relative abundance	1093:1110	relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs,	1093:1195	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	5	20	theme	HMOs	1084:1087	arg1	levels					1052:1057	levels	1052:1057	levels of individual breastmilk HMOs	1052:1087	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	5	21	theme	breastmilk	1073:1082	arg1	HMOs					1084:1087	individual breastmilk HMOs	1062:1087	individual breastmilk HMOs	1062:1087	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	4	22	theme	delivery	880:887	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	23	theme	healthy	458:464	arg1	infants					477:483	healthy, breastfed infants	458:483	healthy, breastfed infants	458:483	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	5	24	located	found	1120:1124	arg1	OTUs					1191:1194	the most predominant Bifidobacterium OTUs	1154:1194	the most predominant Bifidobacterium OTUs	1154:1194	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	5	24	located	found	1120:1124	arg1	faeces					1136:1141	infant faeces	1129:1141	infant faeces	1129:1141	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	5	24	located	found	1120:1124	arg2	OTUs					1115:1118	OTUs	1115:1118	OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs,	1115:1195	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	6	25	theme	faecal	1232:1237	arg1	concentration					1239:1251	The faecal concentration	1228:1251	The faecal concentration of HMOs	1228:1259	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	3	26	theme	Lactation	518:526	arg1	duration					528:535	Lactation duration	518:535	Lactation duration	518:535	Lactation duration had a significant effect on breastmilk HMO content, which decreased with time, except for 3-fucosyllactose (3FL) and Lacto-N-fucopentaose III (LNFP III).					
32144305	4	27	theme	LNFP	826:829	arg1	concentration					835:847	breastmilk LNFP III concentration	815:847	breastmilk LNFP III concentration at two weeks	815:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	1	28	theme	gut	164:166	arg1	development					179:189	gut microbiota development	164:189	gut microbiota development	164:189	Several factors affect gut microbiota development in early life, among which breastfeeding plays a key role.					
32144305	6	29	dep	Escherichia-Shigella	1404:1423	arg1	Parabacteroides					1387:1401	Parabacteroides	1387:1401	Parabacteroides	1387:1401	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	4	30	theme	sex	973:975	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	30	theme	sex	973:975	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	30	theme	sex	973:975	arg1	concentrations					899:912	concentrations	899:912	concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age	899:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	31	theme	oligosaccharides	363:378	arg1	concentrations					325:338	concentrations	325:338	concentrations of selected human milk oligosaccharides	325:378	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	0	32	theme	breastmilk	24:33	arg1	oligosaccharides					35:50	breastmilk oligosaccharides	24:50	breastmilk oligosaccharides	24:50	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	4	33	theme	infant	966:971	arg1	LNH					998:1000	LNH	998:1000	LNH	998:1000	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	33	theme	infant	966:971	arg1	sex					973:975	infant sex	966:975	infant sex	966:975	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	34	theme	infant	402:407	arg1	faeces					409:414	infant faeces	402:414	infant faeces	402:414	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	2	35	theme	mother-infant	265:277	arg1	pairs					279:283	24 mother-infant pairs	262:283	24 mother-infant pairs	262:283	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	6	36	theme	taxa	1579:1582	arg1	importance					1559:1568	the likely importance	1548:1568	the likely importance of these taxa for HMO metabolism in vivo	1548:1609	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	6	37	theme	HMO	1588:1590	arg1	metabolism					1592:1601	HMO metabolism	1588:1601	HMO metabolism in vivo	1588:1609	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	4	38	from	sex	875:877	arg1	milk					943:946	milk	943:946	milk	943:946	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	38	from	sex	875:877	arg1	weeks					955:959	six weeks	951:959	six weeks	951:959	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	38	from	sex	875:877	arg1	milk					1006:1009	milk	1006:1009	milk	1006:1009	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	38	from	sex	875:877	arg1	weeks					1017:1021	12 weeks	1014:1021	12 weeks of age	1014:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	39	theme	12	501:502	arg1	weeks					504:508	two, six and 12 weeks	488:508	weeks	504:508	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	4	40	from	concentrations	899:912	arg1	milk					943:946	milk	943:946	milk	943:946	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	40	from	concentrations	899:912	arg1	weeks					955:959	six weeks	951:959	six weeks	951:959	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	40	from	concentrations	899:912	arg1	milk					1006:1009	milk	1006:1009	milk	1006:1009	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	40	from	concentrations	899:912	arg1	weeks					1017:1021	12 weeks	1014:1021	12 weeks of age	1014:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	3	41	theme	significant	543:553	arg1	effect					555:560	a significant effect	541:560	a significant effect	541:560	Lactation duration had a significant effect on breastmilk HMO content, which decreased with time, except for 3-fucosyllactose (3FL) and Lacto-N-fucopentaose III (LNFP III).					
32144305	1	42	theme	microbiota	168:177	arg1	development					179:189	gut microbiota development	164:189	gut microbiota development	164:189	Several factors affect gut microbiota development in early life, among which breastfeeding plays a key role.					
32144305	1	43	theme	early	194:198	arg1	life					200:203	early life	194:203	early life	194:203	Several factors affect gut microbiota development in early life, among which breastfeeding plays a key role.					
32144305	6	44	theme	HMOs	1256:1259	arg1	concentration					1239:1251	The faecal concentration	1228:1251	The faecal concentration of HMOs	1228:1259	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	4	45	from	sialyllactose	919:931	arg1	milk					943:946	milk	943:946	milk	943:946	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	45	from	sialyllactose	919:931	arg1	milk					1006:1009	milk	1006:1009	milk	1006:1009	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	46	from	weeks	856:860	arg1	concentration					835:847	breastmilk LNFP III concentration	815:847	breastmilk LNFP III concentration at two weeks	815:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	46	from	weeks	856:860	arg1	delivery					802:809	delivery	802:809	delivery	802:809	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	46	from	weeks	856:860	arg1	mode					794:797	mode	794:797	mode of delivery and breastmilk LNFP III concentration at two weeks	794:860	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	47	from	sex	973:975	arg1	milk					943:946	milk	943:946	milk	943:946	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	47	from	sex	973:975	arg1	milk					1006:1009	milk	1006:1009	milk	1006:1009	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	48	theme	age	1026:1028	arg1	weeks					1017:1021	12 weeks	1014:1021	12 weeks of age	1014:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	49	theme	infant	759:764	arg1	age					766:768	infant age	759:768	infant age	759:768	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	50	theme	microbiota	432:441	arg1	composition					443:453	the faecal microbiota composition	421:453	the faecal microbiota composition in healthy, breastfed infants	421:483	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	0	51	theme	age	136:138	arg1	weeks					127:131	two, six, and twelve weeks	106:131	two, six, and twelve weeks of age	106:138	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	6	52	theme	OTUs	1351:1354	arg1	abundance					1338:1346	relative abundance	1329:1346	relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis	1329:1534	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	0	53	theme	faecal	56:61	arg1	microbiota					63:72	faecal microbiota	56:72	faecal microbiota	56:72	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	2	54	dep	healthy	458:464	arg1	breastfed					467:475	breastfed	467:475	breastfed	467:475	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	6	55	theme	likely	1552:1557	arg1	importance					1559:1568	the likely importance	1548:1568	the likely importance of these taxa for HMO metabolism in vivo	1548:1609	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	1	56	from	development	179:189	arg1	life					200:203	early life	194:203	early life	194:203	Several factors affect gut microbiota development in early life, among which breastfeeding plays a key role.					
32144305	5	57	theme	predominant	1163:1173	arg1	OTUs					1191:1194	the most predominant Bifidobacterium OTUs	1154:1194	the most predominant Bifidobacterium OTUs	1154:1194	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	4	58	from	milk	1006:1009	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	58	from	milk	1006:1009	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	58	from	milk	1006:1009	arg1	concentrations					899:912	concentrations	899:912	concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age	899:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	LNH					998:1000	LNH	998:1000	LNH	998:1000	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	sialyllactose					919:931	3'sialyllactose	917:931	3'sialyllactose (3'SL) in milk at six weeks	917:959	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	sex					973:975	infant sex	966:975	infant sex	966:975	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	concentrations					899:912	concentrations	899:912	concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age	899:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	Lacto-N-hexaose					981:995	Lacto-N-hexaose	981:995	Lacto-N-hexaose	981:995	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	59	from	weeks	1017:1021	arg1	SL					936:937	3'SL	934:937	3'SL	934:937	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	3	60	theme	HMO	576:578	arg1	content					580:586	breastmilk HMO content	565:586	breastmilk HMO content	565:586	Lactation duration had a significant effect on breastmilk HMO content, which decreased with time, except for 3-fucosyllactose (3FL) and Lacto-N-fucopentaose III (LNFP III).					
32144305	2	61	dep	investigate	288:298	arg1	HMOs					381:384	HMOs	381:384	HMOs	381:384	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	5	62	theme	infant	1129:1134	arg1	OTUs					1191:1194	the most predominant Bifidobacterium OTUs	1154:1194	the most predominant Bifidobacterium OTUs	1154:1194	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	5	62	theme	infant	1129:1134	arg1	faeces					1136:1141	infant faeces	1129:1141	infant faeces	1129:1141	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	0	63	from	weeks	127:131	arg1	infants					95:101	healthy breastfed infants	77:101	healthy breastfed infants at two, six, and twelve weeks of age	77:138	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	0	63	from	weeks	127:131	arg1	association					4:14	The association	0:14	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.	0:139	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	2	64	theme	age	513:515	arg1	weeks					504:508	two, six and 12 weeks	488:508	weeks	504:508	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	2	65	from	breastmilk	390:399	arg1	infants					477:483	healthy, breastfed infants	458:483	healthy, breastfed infants	458:483	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	4	66	from	milk	943:946	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	66	from	milk	943:946	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	66	from	milk	943:946	arg1	concentrations					899:912	concentrations	899:912	concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age	899:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	6	67	theme	relative	1329:1336	arg1	abundance					1338:1346	relative abundance	1329:1346	relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis	1329:1534	The faecal concentration of HMOs decreased with age and were strongly and negatively correlated with relative abundance of OTUs within genera Bifidobacterium, Parabacteroides, Escherichia-Shigella, Bacteroides, Actinomyces, Veillonella, Lachnospiraceae Incertae Sedis, and Erysipelotrichaceae Incertae Sedis, indicating the likely importance of these taxa for HMO metabolism in vivo.					
32144305	4	68	from	weeks	955:959	arg1	LNH					998:1000	LNH	998:1000	LNH	998:1000	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	sialyllactose					919:931	3'sialyllactose	917:931	3'sialyllactose (3'SL) in milk at six weeks	917:959	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	sex					973:975	infant sex	966:975	infant sex	966:975	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	concentrations					899:912	concentrations	899:912	concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age	899:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	Lacto-N-hexaose					981:995	Lacto-N-hexaose	981:995	Lacto-N-hexaose	981:995	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	68	from	weeks	955:959	arg1	SL					936:937	3'SL	934:937	3'SL	934:937	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	0	69	theme	healthy	77:83	arg1	infants					95:101	healthy breastfed infants	77:101	healthy breastfed infants at two, six, and twelve weeks of age	77:138	The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.					
32144305	4	70	from	Lacto-N-hexaose	981:995	arg1	milk					943:946	milk	943:946	milk	943:946	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	70	from	Lacto-N-hexaose	981:995	arg1	milk					1006:1009	milk	1006:1009	milk	1006:1009	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	2	71	theme	milk	358:361	arg1	oligosaccharides					363:378	selected human milk oligosaccharides	343:378	selected human milk oligosaccharides	343:378	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	5	72	with	weak	1202:1205	arg1	age					1223:1225	age	1223:1225	age	1223:1225	Correlations between levels of individual breastmilk HMOs and relative abundance of OTUs found in infant faeces, including the most predominant Bifidobacterium OTUs, were weak and varied with age.					
32144305	4	73	theme	sialyllactose	919:931	arg1	sex					875:877	infant sex	868:877	infant sex	868:877	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	73	theme	sialyllactose	919:931	arg1	mode					889:892	delivery mode	880:892	delivery mode	880:892	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	73	theme	sialyllactose	919:931	arg1	concentrations					899:912	concentrations	899:912	concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age	899:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	3	74	theme	breastmilk	565:574	arg1	content					580:586	breastmilk HMO content	565:586	breastmilk HMO content	565:586	Lactation duration had a significant effect on breastmilk HMO content, which decreased with time, except for 3-fucosyllactose (3FL) and Lacto-N-fucopentaose III (LNFP III).					
32144305	2	75	theme	human	352:356	arg1	oligosaccharides					363:378	selected human milk oligosaccharides	343:378	selected human milk oligosaccharides	343:378	We followed 24 mother-infant pairs to investigate the associations between concentrations of selected human milk oligosaccharides (HMOs) in breastmilk, infant faeces, and the faecal microbiota composition in healthy, breastfed infants at two, six and 12 weeks of age.					
32144305	4	76	from	mode	889:892	arg1	milk					943:946	milk	943:946	milk	943:946	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	76	from	mode	889:892	arg1	weeks					955:959	six weeks	951:959	six weeks	951:959	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	76	from	mode	889:892	arg1	milk					1006:1009	milk	1006:1009	milk	1006:1009	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32144305	4	76	from	mode	889:892	arg1	weeks					1017:1021	12 weeks	1014:1021	12 weeks of age	1014:1028	We confirmed that microbiota composition was strongly influenced by infant age and was associated with mode of delivery and breastmilk LNFP III concentration at two weeks, with infant sex, delivery mode, and concentrations of 3'sialyllactose (3'SL) in milk at six weeks, and infant sex and Lacto-N-hexaose (LNH) in milk at 12 weeks of age.					
32058669	5	0	theme	aseptic	1337:1343	arg1	environment					1359:1369	an appropriate moist aseptic wound healing environment	1316:1369	an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1316:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	4	1	theme	healing	994:1000	arg1	rate					1002:1005	and the wound healing rate	980:1005	rate	1002:1005	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	2	theme	healing	1351:1357	arg1	environment					1359:1369	an appropriate moist aseptic wound healing environment	1316:1369	an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1316:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	6	3	theme	promising	1481:1489	arg1	applications					1491:1502	promising applications	1481:1502	promising applications	1481:1502	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	2	4	theme	improved	569:576	arg1	activity					592:599	improved antibacterial activity	569:599	improved antibacterial activity	569:599	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	5	theme	novel	255:259	arg1	kind					261:264	a novel kind	253:264	a novel kind of promising wound dressing	253:292	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	4	6	theme	PVA/CS/mGO	820:829	arg1	film					841:844	PVA/CS/mGO composite film	820:844	PVA/CS/mGO composite film with 0.5 wt% mGO sheets	820:868	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	7	theme	appropriate	1319:1329	arg1	environment					1359:1369	an appropriate moist aseptic wound healing environment	1316:1369	an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1316:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	6	8	theme	PVA/CS/mGO	1456:1465	arg1	film					1467:1470	this novel composite PVA/CS/mGO film	1435:1470	this novel composite PVA/CS/mGO film	1435:1470	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	2	9	theme	water	511:515	arg1	WVTR					542:545	WVTR	542:545	WVTR	542:545	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	9	theme	water	511:515	arg1	rate					536:539	higher water vapor transmission rate	504:539	higher water vapor transmission rate (WVTR)	504:546	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	3	10	theme	In	682:683	arg1	experiments					690:700	In vivo experiments	682:700	In vivo experiments	682:700	In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization.					
32058669	2	11	theme	wound	279:283	arg1	dressing					285:292	promising wound dressing	269:292	promising wound dressing	269:292	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	6	12	theme	novel	1440:1444	arg1	film					1467:1470	this novel composite PVA/CS/mGO film	1435:1470	this novel composite PVA/CS/mGO film	1435:1470	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	4	13	theme	wound	888:892	arg1	healing					894:900	the best wound healing	879:900	the best wound healing properties	879:911	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	3	14	theme	PVA/CS/mGO	720:729	arg1	film					741:744	the PVA/CS/mGO composite film	716:744	the PVA/CS/mGO composite film	716:744	In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization.					
32058669	4	15	theme	%	857:857	arg1	sheets					863:868	0.5 wt% mGO sheets	851:868	0.5 wt% mGO sheets	851:868	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	2	16	theme	PVA/CS	646:651	arg1	film					653:656	the PVA/CS film	642:656	the PVA/CS film crosslinked by genipin	642:679	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	4	17	theme	PVA/CS	1112:1117	arg1	dressing					1119:1126	the pure PVA/CS dressing	1103:1126	the pure PVA/CS dressing	1103:1126	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	2	18	theme	transmission	523:534	arg1	WVTR					542:545	WVTR	542:545	WVTR	542:545	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	18	theme	transmission	523:534	arg1	rate					536:539	higher water vapor transmission rate	504:539	higher water vapor transmission rate (WVTR)	504:546	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	5	19	theme	properties	1187:1196	arg1	result					1207:1212	a result	1205:1212	a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1205:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	5	19	theme	properties	1187:1196	arg1	mechanism					1144:1152	The underlying mechanism	1129:1152	The underlying mechanism of the accelerated wound healing properties	1129:1196	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	1	20	theme	exudate	130:136	arg1	prerequisites					176:188	prerequisites	176:188	prerequisites for wound dressing	176:207	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	1	20	theme	exudate	130:136	arg1	prevention					142:151	prevention	142:151	prevention of wound infection	142:170	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	1	20	theme	exudate	130:136	arg1	absorption					110:119	Rapid absorption	104:119	Rapid absorption of wound exudate	104:136	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	4	21	with	film	841:844	arg1	sheets					863:868	0.5 wt% mGO sheets	851:868	0.5 wt% mGO sheets	851:868	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	0	22	theme	Oxide	53:57	arg1	Biocomposite					59:70	Graphene Oxide Biocomposite	44:70	Graphene Oxide Biocomposite for Wound Dressing Application	44:101	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	3	23	dep	In	682:683	arg1	vivo					685:688	vivo	685:688	vivo	685:688	In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization.					
32058669	2	24	theme	vapor	517:521	arg1	WVTR					542:545	WVTR	542:545	WVTR	542:545	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	24	theme	vapor	517:521	arg1	rate					536:539	higher water vapor transmission rate	504:539	higher water vapor transmission rate (WVTR)	504:546	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	1	25	theme	wound	156:160	arg1	infection					162:170	wound infection	156:170	wound infection	156:170	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	2	26	theme	mechanical	481:490	arg1	properties					492:501	the required mechanical properties	468:501	the dressing the required mechanical properties	455:501	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	5	27	theme	wound	1173:1177	arg1	healing					1179:1185	the accelerated wound healing	1157:1185	the accelerated wound healing properties	1157:1196	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	0	28	theme	Dressing	82:89	arg1	Application					91:101	Wound Dressing Application	76:101	Wound Dressing Application	76:101	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	2	29	theme	antibacterial	578:590	arg1	activity					592:599	improved antibacterial activity	569:599	improved antibacterial activity	569:599	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	5	30	theme	antibacterial	1230:1242	arg1	ability					1244:1250	the improved antibacterial ability	1217:1250	the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1217:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	2	31	dep	incorporating	310:322	arg1	conferring					444:453	conferring	444:453	conferring the dressing the required mechanical properties	444:501	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	0	32	theme	Novel	0:4	arg1	Poly					6:9	Novel Poly	0:9	Novel Poly(vinyl alcohol)	0:24	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	0	32	theme	Novel	0:4	arg1	alcohol					17:23	vinyl alcohol	11:23	vinyl alcohol	11:23	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	2	33	theme	vinyl	403:407	arg1	matrix					436:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	34	theme	more	606:609	arg1	proliferation					616:628	more cell proliferation	606:628	more cell proliferation	606:628	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	35	theme	poly	398:401	arg1	matrix					436:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	36	dep	dressing	459:466	arg1	properties					492:501	the required mechanical properties	468:501	the dressing the required mechanical properties	455:501	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	1	37	theme	Rapid	104:108	arg1	prerequisites					176:188	prerequisites	176:188	prerequisites for wound dressing	176:207	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	1	37	theme	Rapid	104:108	arg1	prevention					142:151	prevention	142:151	prevention of wound infection	142:170	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	1	37	theme	Rapid	104:108	arg1	absorption					110:119	Rapid absorption	104:119	Rapid absorption of wound exudate	104:136	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	4	38	theme	higher	939:944	arg1	rate					960:963	the 50% higher antibacterial rate	931:963	the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing	931:1126	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	39	theme	wound	1292:1296	arg1	area					1298:1301	the wound area	1288:1301	the wound area	1288:1301	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	2	40	theme	guanidine	342:350	arg1	mGO					384:386	mGO	384:386	mGO	384:386	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	40	theme	guanidine	342:350	arg1	oxide					377:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide	324:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO)	324:387	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	1	41	theme	wound	223:227	arg1	healing					229:235	wound healing	223:235	wound healing	223:235	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	5	42	theme	pathogenic	1265:1274	arg1	bacteria					1276:1283	pathogenic bacteria	1265:1283	pathogenic bacteria on the wound area	1265:1301	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	2	43	theme	-modified	358:366	arg1	mGO					384:386	mGO	384:386	mGO	384:386	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	43	theme	-modified	358:366	arg1	oxide					377:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide	324:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO)	324:387	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	5	44	theme	underlying	1133:1142	arg1	result					1207:1212	a result	1205:1212	a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1205:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	5	44	theme	underlying	1133:1142	arg1	mechanism					1144:1152	The underlying mechanism	1129:1152	The underlying mechanism of the accelerated wound healing properties	1129:1196	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	4	45	theme	wound	988:992	arg1	healing					994:1000	wound healing	988:1000	wound healing	988:1000	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	2	46	theme	PHMG	353:356	arg1	mGO					384:386	mGO	384:386	mGO	384:386	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	46	theme	PHMG	353:356	arg1	oxide					377:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide	324:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO)	324:387	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	4	47	theme	composite	831:839	arg1	film					841:844	PVA/CS/mGO composite film	820:844	PVA/CS/mGO composite film with 0.5 wt% mGO sheets	820:868	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	48	theme	wound	1345:1349	arg1	healing					1351:1357	wound healing	1345:1357	an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1316:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	2	49	theme	promising	269:277	arg1	dressing					285:292	promising wound dressing	269:292	promising wound dressing	269:292	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	1	50	theme	infection	162:170	arg1	prerequisites					176:188	prerequisites	176:188	prerequisites for wound dressing	176:207	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	1	50	theme	infection	162:170	arg1	prevention					142:151	prevention	142:151	prevention of wound infection	142:170	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	1	50	theme	infection	162:170	arg1	absorption					110:119	Rapid absorption	104:119	Rapid absorption of wound exudate	104:136	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	2	51	theme	/chitosan	417:425	arg1	matrix					436:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	52	theme	swelling	554:561	arg1	time					563:566	less swelling time	549:566	less swelling time	549:566	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	5	53	theme	moist	1331:1335	arg1	environment					1359:1369	an appropriate moist aseptic wound healing environment	1316:1369	an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1316:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	2	54	theme	dressing	285:292	arg1	kind					261:264	a novel kind	253:264	a novel kind of promising wound dressing	253:292	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	6	55	theme	composite	1446:1454	arg1	film					1467:1470	this novel composite PVA/CS/mGO film	1435:1470	this novel composite PVA/CS/mGO film	1435:1470	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	1	56	theme	wound	194:198	arg1	dressing					200:207	wound dressing	194:207	wound dressing	194:207	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	2	57	theme	higher	504:509	arg1	WVTR					542:545	WVTR	542:545	WVTR	542:545	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	57	theme	higher	504:509	arg1	rate					536:539	higher water vapor transmission rate	504:539	higher water vapor transmission rate (WVTR)	504:546	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	4	58	theme	best	883:886	arg1	healing					894:900	the best wound healing	879:900	the best wound healing properties	879:911	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	3	59	theme	composite	731:739	arg1	film					741:744	the PVA/CS/mGO composite film	716:744	the PVA/CS/mGO composite film	716:744	In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization.					
32058669	4	60	theme	wt	855:856	arg1	sheets					863:868	0.5 wt% mGO sheets	851:868	0.5 wt% mGO sheets	851:868	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	61	theme	healing	1179:1185	arg1	properties					1187:1196	the accelerated wound healing properties	1157:1196	the accelerated wound healing properties	1157:1196	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	1	62	theme	wound	124:128	arg1	exudate					130:136	wound exudate	124:136	wound exudate	124:136	Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing.					
32058669	4	63	theme	mGO	859:861	arg1	sheets					863:868	0.5 wt% mGO sheets	851:868	0.5 wt% mGO sheets	851:868	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	4	64	theme	control	1069:1075	arg1	group					1077:1081	the control group	1065:1081	the control group	1065:1081	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	3	65	theme	wound	761:765	arg1	healing					767:773	wound healing	761:773	wound healing	761:773	In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization.					
32058669	6	66	theme	wound	1507:1511	arg1	dressing					1513:1520	wound dressing	1507:1520	wound dressing	1507:1520	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	3	67	theme	re-epithelialization	798:817	arg1	enhancement					779:789	enhancement	779:789	enhancement of the re-epithelialization	779:817	In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization.					
32058669	2	68	theme	required	472:479	arg1	properties					492:501	the required mechanical properties	468:501	the dressing the required mechanical properties	455:501	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	0	69	theme	Wound	76:80	arg1	Application					91:101	Wound Dressing Application	76:101	Wound Dressing Application	76:101	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	5	70	theme	accelerated	1161:1171	arg1	healing					1179:1185	the accelerated wound healing	1157:1185	the accelerated wound healing properties	1157:1196	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	6	71	contain	have	1476:1479	arg2	applications					1491:1502	promising applications	1481:1502	promising applications	1481:1502	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	6	71	contain	have	1476:1479	arg1	film					1467:1470	this novel composite PVA/CS/mGO film	1435:1470	this novel composite PVA/CS/mGO film	1435:1470	These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.					
32058669	4	72	theme	healing	894:900	arg1	properties					902:911	the best wound healing properties	879:911	the best wound healing properties	879:911	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	73	theme	improved	1221:1228	arg1	ability					1244:1250	the improved antibacterial ability	1217:1250	the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1217:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	5	74	from	bacteria	1276:1283	arg1	area					1298:1301	the wound area	1288:1301	the wound area	1288:1301	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	4	75	theme	pure	1107:1110	arg1	dressing					1119:1126	the pure PVA/CS dressing	1103:1126	the pure PVA/CS dressing	1103:1126	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	5	76	theme	ability	1244:1250	arg1	result					1207:1212	a result	1205:1212	a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization	1205:1404	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	5	76	theme	ability	1244:1250	arg1	mechanism					1144:1152	The underlying mechanism	1129:1152	The underlying mechanism of the accelerated wound healing properties	1129:1196	The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization.					
32058669	2	77	theme	alcohol	409:415	arg1	matrix					436:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	78	theme	cell	611:614	arg1	proliferation					616:628	more cell proliferation	606:628	more cell proliferation	606:628	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	79	theme	graphene	368:375	arg1	mGO					384:386	mGO	384:386	mGO	384:386	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	79	theme	graphene	368:375	arg1	oxide					377:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide	324:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO)	324:387	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	0	80	theme	vinyl	11:15	arg1	Poly					6:9	Novel Poly	0:9	Novel Poly(vinyl alcohol)	0:24	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	0	80	theme	vinyl	11:15	arg1	alcohol					17:23	vinyl alcohol	11:23	vinyl alcohol	11:23	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	2	81	theme	PVA/CS	428:433	arg1	matrix					436:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	the poly(vinyl alcohol)/chitosan (PVA/CS) matrix	394:441	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	0	82	theme	Graphene	44:51	arg1	Biocomposite					59:70	Graphene Oxide Biocomposite	44:70	Graphene Oxide Biocomposite for Wound Dressing Application	44:101	Novel Poly(vinyl alcohol)/Chitosan/Modified Graphene Oxide Biocomposite for Wound Dressing Application.					
32058669	4	83	theme	antibacterial	946:958	arg1	rate					960:963	the 50% higher antibacterial rate	931:963	the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing	931:1126	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32058669	2	84	theme	polyhexamethylene	324:340	arg1	mGO					384:386	mGO	384:386	mGO	384:386	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	2	84	theme	polyhexamethylene	324:340	arg1	oxide					377:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide	324:381	polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO)	324:387	In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin.					
32058669	4	85	theme	mouse	1014:1018	arg1	rate					1002:1005	and the wound healing rate	980:1005	rate	1002:1005	PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing.					
32688314	5	0	theme	emu/g	900:904	arg1	magnetization					877:889	a saturation magnetization	864:889	a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property	864:942	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	7	1	theme	Pb	1124:1125	arg1	removal					1131:1137	Pb(II) removal	1124:1137	Pb(II) removal	1124:1137	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	8	2	theme	kinetic	1440:1446	arg1	model					1448:1452	intraparticle diffusion kinetic model	1416:1452	intraparticle diffusion kinetic model	1416:1452	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	3	3	theme	EDAC/NHS	529:536	arg1	activation					538:547	EDAC/NHS activation	529:547	EDAC/NHS activation	529:547	Subsequently, they were covalently bonded to chitosan via carbodiimide chemistry using EDAC/NHS activation.					
32688314	4	4	theme	chemical	613:620	arg1	conjugation					622:632	its chemical conjugation	609:632	its chemical conjugation	609:632	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	7	5	theme	optimum	1084:1090	arg1	conditions					1092:1101	The optimum conditions	1080:1101	The optimum conditions to achieve 92.15% of Pb(II) removal	1080:1137	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	4	6	theme	bond	718:721	arg1	detachment					693:702	the ultimate chitosan detachment	671:702	the ultimate chitosan detachment of a physical bond	671:721	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	10	7	theme	treatment	1672:1680	arg1	technology					1682:1691	wastewater treatment technology	1661:1691	wastewater treatment technology	1661:1691	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	0	8	theme	magnetite	83:91	arg1	nano-biocomposite					93:109	chitosan-conjugated magnetite nano-biocomposite	63:109	chitosan-conjugated magnetite nano-biocomposite using response surface methodology	63:144	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	6	9	theme	design	1009:1014	arg1	framework					1022:1030	a central composite design (CCD) framework	989:1030	a central composite design (CCD) framework	989:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	0	10	theme	chitosan-conjugated	63:81	arg1	nano-biocomposite					93:109	chitosan-conjugated magnetite nano-biocomposite	63:109	chitosan-conjugated magnetite nano-biocomposite using response surface methodology	63:144	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	7	11	theme	1.04 g/L	1212:1219	arg1	time					1235:1238	a contact time	1225:1238	a contact time of 59.92 min	1225:1251	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	7	11	theme	1.04 g/L	1212:1219	arg1	concentration					1195:1207	the nano-adsorbent concentration	1176:1207	the nano-adsorbent concentration of 1.04 g/L	1176:1219	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	3	12	theme	carbodiimide	500:511	arg1	chemistry					513:521	carbodiimide chemistry	500:521	carbodiimide chemistry using EDAC/NHS activation	500:547	Subsequently, they were covalently bonded to chitosan via carbodiimide chemistry using EDAC/NHS activation.					
32688314	10	13	theme	synthetic	1578:1586	arg1	nano-adsorbent					1624:1637	an ideal nano-adsorbent	1615:1637	an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology	1615:1691	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	10	13	theme	synthetic	1578:1586	arg1	nano-biocomposite					1588:1604	the proposed synthetic nano-biocomposite	1565:1604	the proposed synthetic nano-biocomposite	1565:1604	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	4	14	theme	waste	803:807	arg1	production					809:818	no secondary waste production	790:818	no secondary waste production	790:818	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	10	15	theme	Pb	1643:1644	arg1	removal					1650:1656	Pb(II) removal	1643:1656	Pb(II) removal	1643:1656	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	4	16	theme	chitosan	684:691	arg1	detachment					693:702	the ultimate chitosan detachment	671:702	the ultimate chitosan detachment of a physical bond	671:721	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	6	17	theme	composite	999:1007	arg1	framework					1022:1030	a central composite design (CCD) framework	989:1030	a central composite design (CCD) framework	989:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	5	18	theme	superparamagnetic	917:933	arg1	property					935:942	a superparamagnetic property	915:942	a superparamagnetic property	915:942	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	6	19	theme	CCD	1017:1019	arg1	framework					1022:1030	a central composite design (CCD) framework	989:1030	a central composite design (CCD) framework	989:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	9	20	theme	adsorption	1483:1492	arg1	192.308 mg/g					1524:1535	192.308 mg/g	1524:1535	192.308 mg/g	1524:1535	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	9	20	theme	adsorption	1483:1492	arg1	capacity					1494:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	4	21	theme	secondary	793:801	arg1	production					809:818	no secondary waste production	790:818	no secondary waste production	790:818	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	1	22	theme	nano-biocomposite	209:225	arg1	performance					183:193	the performance	179:193	the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions	179:308	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	4	23	theme	ultimate	675:682	arg1	detachment					693:702	the ultimate chitosan detachment	671:702	the ultimate chitosan detachment of a physical bond	671:721	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	4	24	with	separation	774:783	arg1	production					809:818	no secondary waste production	790:818	no secondary waste production	790:818	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	7	25	theme	nano-adsorbent	1180:1193	arg1	concentration					1195:1207	the nano-adsorbent concentration	1176:1207	the nano-adsorbent concentration of 1.04 g/L	1176:1219	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	6	26	with	methodology	966:976	arg1	framework					1022:1030	a central composite design (CCD) framework	989:1030	a central composite design (CCD) framework	989:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	10	27	theme	proposed	1569:1576	arg1	nano-adsorbent					1624:1637	an ideal nano-adsorbent	1615:1637	an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology	1615:1691	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	10	27	theme	proposed	1569:1576	arg1	nano-biocomposite					1588:1604	the proposed synthetic nano-biocomposite	1565:1604	the proposed synthetic nano-biocomposite	1565:1604	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	0	28	theme	Process	0:6	arg1	optimization					8:19	Process optimization	0:19	Process optimization	0:19	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	6	29	theme	central	991:997	arg1	framework					1022:1030	a central composite design (CCD) framework	989:1030	a central composite design (CCD) framework	989:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	6	30	used	used	1036:1039	arg2	RSM					979:981	RSM	979:981	RSM	979:981	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	6	30	used	used	1036:1039	arg2	methodology					966:976	The response surface methodology	945:976	The response surface methodology (RSM) with a central composite design (CCD) framework	945:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	5	31	theme	saturation	866:875	arg1	magnetization					877:889	a saturation magnetization	864:889	a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property	864:942	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	8	32	theme	isotherm	1329:1336	arg1	model					1338:1342	the Langmuir adsorption isotherm model	1305:1342	the Langmuir adsorption isotherm model	1305:1342	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	8	33	theme	adsorption	1318:1327	arg1	model					1338:1342	the Langmuir adsorption isotherm model	1305:1342	the Langmuir adsorption isotherm model	1305:1342	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	4	34	theme	detachment	693:702	arg1	weakness					659:666	the weakness	655:666	the weakness of the ultimate chitosan detachment of a physical bond	655:721	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	7	35	theme	removal	1131:1137	arg1	removal					1131:1137	Pb(II) removal	1124:1137	Pb(II) removal	1124:1137	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	7	35	theme	removal	1131:1137	arg1	%					1119:1119	92.15%	1114:1119	92.15% of Pb(II) removal	1114:1137	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	8	36	theme	isotherm	1269:1276	arg1	data					1278:1281	Our adsorption isotherm data	1254:1281	Our adsorption isotherm data	1254:1281	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	0	37	theme	surface	126:132	arg1	methodology					134:144	response surface methodology	117:144	response surface methodology	117:144	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	5	38	theme	∼10 nm	851:856	arg1	diameter					839:846	a diameter	837:846	a diameter of ∼10 nm	837:856	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	0	39	theme	response	117:124	arg1	methodology					134:144	response surface methodology	117:144	response surface methodology	117:144	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	8	40	theme	diffusion	1430:1438	arg1	model					1448:1452	intraparticle diffusion kinetic model	1416:1452	intraparticle diffusion kinetic model	1416:1452	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	4	41	theme	magnetic	765:772	arg1	separation					774:783	magnetic separation	765:783	magnetic separation with no secondary waste production	765:818	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	1	42	theme	magnetic	200:207	arg1	nanoparticle					258:269	chitosan conjugated magnetite nanoparticle	228:269	chitosan conjugated magnetite nanoparticle (CH-MNP)	228:278	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	42	theme	magnetic	200:207	arg1	nano-biocomposite					209:225	a magnetic nano-biocomposite	198:225	a magnetic nano-biocomposite	198:225	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	0	43	theme	Pb	37:38	arg1	ions					44:47	Pb(II) ions	37:47	Pb(II) ions	37:47	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	4	44	theme	nano-biocomposite	588:604	arg1	advantages					569:578	the notable advantages	557:578	the notable advantages of this nano-biocomposite	557:604	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	0	45	from	adsorption	49:58	arg1	nano-biocomposite					93:109	chitosan-conjugated magnetite nano-biocomposite	63:109	chitosan-conjugated magnetite nano-biocomposite using response surface methodology	63:144	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	8	46	theme	equilibrium	1353:1363	arg1	data					1365:1368	the equilibrium data	1349:1368	the equilibrium data	1349:1368	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	5	47	theme	76.01	894:898	arg1	emu/g					900:904	76.01 emu/g	894:904	76.01 emu/g ensuring a superparamagnetic property	894:942	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	2	48	theme	citric	429:434	arg1	acid					436:439	citric acid	429:439	citric acid	429:439	The magnetite nanoparticles were synthesized through a controlled co-precipitation technique and were stabilized with citric acid.					
32688314	4	49	theme	physical	709:716	arg1	bond					718:721	a physical bond	707:721	a physical bond	707:721	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	1	50	theme	lead	300:303	arg1	ions					305:308	lead ions	300:308	lead ions	300:308	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	7	51	with	pH	1161:1162	arg1	time					1235:1238	a contact time	1225:1238	a contact time of 59.92 min	1225:1251	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	7	51	with	pH	1161:1162	arg1	concentration					1195:1207	the nano-adsorbent concentration	1176:1207	the nano-adsorbent concentration of 1.04 g/L	1176:1219	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	6	52	theme	surface	958:964	arg1	RSM					979:981	RSM	979:981	RSM	979:981	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	6	52	theme	surface	958:964	arg1	methodology					966:976	The response surface methodology	945:976	The response surface methodology (RSM) with a central composite design (CCD) framework	945:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	1	53	theme	ions	305:308	arg1	removal					289:295	the removal	285:295	the removal of lead ions	285:308	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	5	54	contain	had	833:835	arg1	CH-MNPs					825:831	The CH-MNPs	821:831	The CH-MNPs	821:831	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	5	54	contain	had	833:835	arg2	diameter					839:846	a diameter	837:846	a diameter of ∼10 nm	837:856	The CH-MNPs had a diameter of ∼10 nm, with a saturation magnetization of 76.01 emu/g ensuring a superparamagnetic property.					
32688314	9	55	theme	maximum	1459:1465	arg1	192.308 mg/g					1524:1535	192.308 mg/g	1524:1535	192.308 mg/g	1524:1535	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	9	55	theme	maximum	1459:1465	arg1	capacity					1494:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	10	56	from	nano-adsorbent	1624:1637	arg1	technology					1682:1691	wastewater treatment technology	1661:1691	wastewater treatment technology	1661:1691	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	9	57	theme	Pb	1476:1477	arg1	192.308 mg/g					1524:1535	192.308 mg/g	1524:1535	192.308 mg/g	1524:1535	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	9	57	theme	Pb	1476:1477	arg1	capacity					1494:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	4	58	theme	advantages	569:578	arg1	advantages					569:578	the notable advantages	557:578	the notable advantages of this nano-biocomposite	557:604	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	4	58	theme	advantages	569:578	arg1	One					550:552	One	550:552	One	550:552	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	7	59	theme	59.92 min	1243:1251	arg1	time					1235:1238	a contact time	1225:1238	a contact time of 59.92 min	1225:1251	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	7	59	theme	59.92 min	1243:1251	arg1	concentration					1195:1207	the nano-adsorbent concentration	1176:1207	the nano-adsorbent concentration of 1.04 g/L	1176:1219	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	8	60	theme	intraparticle	1416:1428	arg1	model					1448:1452	intraparticle diffusion kinetic model	1416:1452	intraparticle diffusion kinetic model	1416:1452	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	4	61	theme	notable	561:567	arg1	advantages					569:578	the notable advantages	557:578	the notable advantages of this nano-biocomposite	557:604	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	10	62	theme	ideal	1618:1622	arg1	nano-adsorbent					1624:1637	an ideal nano-adsorbent	1615:1637	an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology	1615:1691	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	10	62	theme	ideal	1618:1622	arg1	nano-biocomposite					1588:1604	the proposed synthetic nano-biocomposite	1565:1604	the proposed synthetic nano-biocomposite	1565:1604	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	8	63	theme	pseudo-second-order	1383:1401	arg1	kinetics					1403:1410	the pseudo-second-order kinetics	1379:1410	the pseudo-second-order kinetics	1379:1410	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	4	64	theme	encouraging	739:749	arg1	candidate					751:759	an encouraging candidate	736:759	an encouraging candidate for magnetic separation with no secondary waste production	736:818	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	10	65	theme	wastewater	1661:1670	arg1	technology					1682:1691	wastewater treatment technology	1661:1691	wastewater treatment technology	1661:1691	These results suggest that the proposed synthetic nano-biocomposite is quite an ideal nano-adsorbent for Pb(II) removal in wastewater treatment technology.					
32688314	1	66	theme	chitosan	228:235	arg1	CH-MNP					272:277	CH-MNP	272:277	CH-MNP	272:277	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	66	theme	chitosan	228:235	arg1	nanoparticle					258:269	chitosan conjugated magnetite nanoparticle	228:269	chitosan conjugated magnetite nanoparticle (CH-MNP)	228:278	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	66	theme	chitosan	228:235	arg1	nano-biocomposite					209:225	a magnetic nano-biocomposite	198:225	a magnetic nano-biocomposite	198:225	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	8	67	theme	Langmuir	1309:1316	arg1	adsorption					1318:1327	Langmuir adsorption	1309:1327	the Langmuir adsorption isotherm model	1305:1342	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	4	68	contain	have	650:653	arg1	conjugation					622:632	its chemical conjugation	609:632	its chemical conjugation	609:632	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	4	68	contain	have	650:653	arg2	weakness					659:666	the weakness	655:666	the weakness of the ultimate chitosan detachment of a physical bond	655:721	One of the notable advantages of this nano-biocomposite is its chemical conjugation, which does not have the weakness of the ultimate chitosan detachment of a physical bond and makes it an encouraging candidate for magnetic separation with no secondary waste production.					
32688314	2	69	theme	magnetite	315:323	arg1	nanoparticles					325:337	The magnetite nanoparticles	311:337	The magnetite nanoparticles	311:337	The magnetite nanoparticles were synthesized through a controlled co-precipitation technique and were stabilized with citric acid.					
32688314	1	70	theme	conjugated	237:246	arg1	CH-MNP					272:277	CH-MNP	272:277	CH-MNP	272:277	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	70	theme	conjugated	237:246	arg1	nanoparticle					258:269	chitosan conjugated magnetite nanoparticle	228:269	chitosan conjugated magnetite nanoparticle (CH-MNP)	228:278	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	70	theme	conjugated	237:246	arg1	nano-biocomposite					209:225	a magnetic nano-biocomposite	198:225	a magnetic nano-biocomposite	198:225	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	0	71	theme	ions	44:47	arg1	modeling					25:32	modeling	25:32	modeling	25:32	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	0	71	theme	ions	44:47	arg1	optimization					8:19	Process optimization	0:19	Process optimization	0:19	Process optimization and modeling of Pb(II) ions adsorption on chitosan-conjugated magnetite nano-biocomposite using response surface methodology.					
32688314	9	72	theme	Langmuir	1467:1474	arg1	192.308 mg/g					1524:1535	192.308 mg/g	1524:1535	192.308 mg/g	1524:1535	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	9	72	theme	Langmuir	1467:1474	arg1	capacity					1494:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity	1455:1501	The maximum Langmuir Pb(II) adsorption capacity was calculated to be 192.308 mg/g.					
32688314	7	73	dep	conditions	1092:1101	arg1	achieve					1106:1112	achieve	1106:1112	to achieve 92.15% of Pb(II) removal	1103:1137	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	6	74	theme	adsorption	1060:1069	arg1	process					1071:1077	the adsorption process	1056:1077	the adsorption process	1056:1077	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	2	75	theme	co-precipitation	377:392	arg1	technique					394:402	a controlled co-precipitation technique	364:402	a controlled co-precipitation technique	364:402	The magnetite nanoparticles were synthesized through a controlled co-precipitation technique and were stabilized with citric acid.					
32688314	8	76	theme	adsorption	1258:1267	arg1	data					1278:1281	Our adsorption isotherm data	1254:1281	Our adsorption isotherm data	1254:1281	Our adsorption isotherm data were fitted well with the Langmuir adsorption isotherm model, and the equilibrium data followed the pseudo-second-order kinetics and intraparticle diffusion kinetic model.					
32688314	1	77	theme	magnetite	248:256	arg1	CH-MNP					272:277	CH-MNP	272:277	CH-MNP	272:277	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	77	theme	magnetite	248:256	arg1	nanoparticle					258:269	chitosan conjugated magnetite nanoparticle	228:269	chitosan conjugated magnetite nanoparticle (CH-MNP)	228:278	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	1	77	theme	magnetite	248:256	arg1	nano-biocomposite					209:225	a magnetic nano-biocomposite	198:225	a magnetic nano-biocomposite	198:225	This study aimed to investigate the performance of a magnetic nano-biocomposite, chitosan conjugated magnetite nanoparticle (CH-MNP), for the removal of lead ions.					
32688314	6	78	theme	response	949:956	arg1	RSM					979:981	RSM	979:981	RSM	979:981	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	6	78	theme	response	949:956	arg1	methodology					966:976	The response surface methodology	945:976	The response surface methodology (RSM) with a central composite design (CCD) framework	945:1030	The response surface methodology (RSM) with a central composite design (CCD) framework was used for optimizing the adsorption process.					
32688314	7	79	theme	contact	1227:1233	arg1	time					1235:1238	a contact time	1225:1238	a contact time of 59.92 min	1225:1251	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
32688314	2	80	theme	controlled	366:375	arg1	technique					394:402	a controlled co-precipitation technique	364:402	a controlled co-precipitation technique	364:402	The magnetite nanoparticles were synthesized through a controlled co-precipitation technique and were stabilized with citric acid.					
32688314	7	81	theme	6.1	1167:1169	arg1	pH					1161:1162	a pH	1159:1162	a pH of 6.1	1159:1169	The optimum conditions to achieve 92.15% of Pb(II) removal were found to be at a pH of 6.1 with the nano-adsorbent concentration of 1.04 g/L and a contact time of 59.92 min.					
33774759	10	0	theme	genome	1387:1392	arg1	analysis					1394:1401	genome analysis	1387:1401	genome analysis	1387:1401	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	9	1	theme	M1531T	1119:1124	arg1	lipids					1274:1279	four unidentified lipids	1256:1279	four unidentified lipids	1256:1279	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	1	theme	M1531T	1119:1124	arg1	phosphatidylethanolamine					1167:1190	phosphatidylethanolamine	1167:1190	phosphatidylethanolamine	1167:1190	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	1	theme	M1531T	1119:1124	arg1	aminolipid					1241:1250	one unidentified aminolipid	1224:1250	one unidentified aminolipid	1224:1250	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	1	theme	M1531T	1119:1124	arg1	phosphatidylglycerol					1145:1164	phosphatidylglycerol	1145:1164	phosphatidylglycerol	1145:1164	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	1	theme	M1531T	1119:1124	arg1	phospholipid					1210:1221	one unidentified phospholipid	1193:1221	one unidentified phospholipid	1193:1221	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	1	theme	M1531T	1119:1124	arg1	lipids					1102:1107	The polar lipids	1092:1107	The polar lipids of strain M1531T	1092:1124	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	8	2	contain	had	915:917	arg2	quinone					953:959	the predominant isoprenoid quinone	926:959	the predominant isoprenoid quinone	926:959	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	2	contain	had	915:917	arg2	Q-8					919:921	Q-8	919:921	Q-8	919:921	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	2	contain	had	915:917	arg1	M1531T					908:913	Strain M1531T	901:913	Strain M1531T	901:913	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	7	3	theme	related	879:885	arg1	species					892:898	the closely related type species	867:898	the closely related type species	867:898	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	9	4	theme	unidentified	1228:1239	arg1	aminolipid					1241:1250	one unidentified aminolipid	1224:1250	one unidentified aminolipid	1224:1250	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	4	theme	unidentified	1228:1239	arg1	lipids					1102:1107	The polar lipids	1092:1107	The polar lipids of strain M1531T	1092:1124	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	10	5	theme	Thalassotalea	1491:1503	arg1	sp					1514:1515	the name Thalassotalea algicola sp	1482:1515	the name Thalassotalea algicola sp	1482:1515	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	2	6	theme	anaerobic	107:115	arg1	bacterium					117:125	A facultatively anaerobic bacterium	91:125	A facultatively anaerobic bacterium	91:125	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	2	6	theme	anaerobic	107:115	arg1	M1531T					135:140	strain M1531T	128:140	strain M1531T	128:140	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	5	7	theme	close	539:543	arg1	relative					545:552	close relative	539:552	close relative with Thalassotalea euphylliae KCTC 42743T	539:594	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	6	8	theme	%	690:690	arg1	genes					716:720	four protein-coding genes	696:720	four protein-coding genes related to the degradation of alginate	696:759	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	6	8	theme	%	690:690	arg1	size					641:644	a genome size	632:644	a genome size of 4,061,950 bp	632:660	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	6	8	theme	%	690:690	arg1	content					671:677	a G + C content	663:677	a G + C content of 39.1 mol%	663:690	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	10	9	theme	phenotypic	1314:1323	arg1	characterization					1341:1356	the phenotypic, chemotaxonomic characterization	1310:1356	the phenotypic, chemotaxonomic characterization	1310:1356	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	7	10	theme	type	887:890	arg1	species					892:898	the closely related type species	867:898	the closely related type species	867:898	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	9	11	theme	polar	1096:1100	arg1	lipids					1274:1279	four unidentified lipids	1256:1279	four unidentified lipids	1256:1279	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	11	theme	polar	1096:1100	arg1	phosphatidylethanolamine					1167:1190	phosphatidylethanolamine	1167:1190	phosphatidylethanolamine	1167:1190	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	11	theme	polar	1096:1100	arg1	aminolipid					1241:1250	one unidentified aminolipid	1224:1250	one unidentified aminolipid	1224:1250	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	11	theme	polar	1096:1100	arg1	phosphatidylglycerol					1145:1164	phosphatidylglycerol	1145:1164	phosphatidylglycerol	1145:1164	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	11	theme	polar	1096:1100	arg1	phospholipid					1210:1221	one unidentified phospholipid	1193:1221	one unidentified phospholipid	1193:1221	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	11	theme	polar	1096:1100	arg1	lipids					1102:1107	The polar lipids	1092:1107	The polar lipids of strain M1531T	1092:1124	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	5	12	theme	16S	498:500	arg1	sequence					512:519	the 16S rRNA gene sequence	494:519	the 16S rRNA gene sequence	494:519	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	7	13	with	%	860:860	arg1	species					892:898	the closely related type species	867:898	the closely related type species	867:898	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	3	14	theme	single	303:308	arg1	flagellum					316:324	a single polar flagellum	301:324	a single polar flagellum	301:324	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	5	15	theme	rRNA	502:505	arg1	sequence					512:519	the 16S rRNA gene sequence	494:519	the 16S rRNA gene sequence	494:519	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	3	16	dep	Gram-stain-negative	249:267	arg1	motile					282:287	motile	282:287	motile	282:287	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	3	16	dep	Gram-stain-negative	249:267	arg1	rod-shaped					270:279	rod-shaped	270:279	rod-shaped	270:279	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	4	17	theme	%	407:407	arg1	NaCl					415:418	2% (w/v) NaCl	406:418	2% (w/v) NaCl	406:418	Optimum growth occurred at 30 °C, with 2% (w/v) NaCl and at pH 6.5-7.0.					
33774759	5	18	theme	sequence	512:519	arg1	analysis					482:489	phylogenetic analysis	469:489	phylogenetic analysis of the 16S rRNA gene sequence	469:519	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	5	19	theme	gene	507:510	arg1	sequence					512:519	the 16S rRNA gene sequence	494:519	the 16S rRNA gene sequence	494:519	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	5	20	dep	result	459:464	arg1	the					442:444	the	442:444	the	442:444	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	5	20	dep	result	459:464	arg1	basis					446:450	basis	446:450	basis	446:450	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	9	21	theme	unidentified	1261:1272	arg1	lipids					1274:1279	four unidentified lipids	1256:1279	four unidentified lipids	1256:1279	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	21	theme	unidentified	1261:1272	arg1	lipids					1102:1107	The polar lipids	1092:1107	The polar lipids of strain M1531T	1092:1124	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	10	22	theme	strain	1404:1409	arg1	M1531T					1411:1416	strain M1531T	1404:1416	strain M1531T	1404:1416	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	6	23	theme	alginate	752:759	arg1	degradation					737:747	the degradation	733:747	the degradation of alginate	733:759	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	8	24	dep	Features	982:989	arg1	3					991:991	3	991:991	3	991:991	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	24	dep	Features	982:989	arg1	ω6c					1010:1012	ω6c	1010:1012	ω6c	1010:1012	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	6	25	theme	G + C	665:669	arg1	content					671:677	a G + C content	663:677	a G + C content of 39.1 mol%	663:690	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	10	26	theme	name	1486:1489	arg1	sp					1514:1515	the name Thalassotalea algicola sp	1482:1515	the name Thalassotalea algicola sp	1482:1515	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	0	27	theme	algicola	14:21	arg1	sp					23:24	Thalassotalea algicola sp	0:24	Thalassotalea algicola sp.	0:25	Thalassotalea algicola sp.					
33774759	2	28	theme	strain	128:133	arg1	bacterium					117:125	A facultatively anaerobic bacterium	91:125	A facultatively anaerobic bacterium	91:125	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	2	28	theme	strain	128:133	arg1	M1531T					135:140	strain M1531T	128:140	strain M1531T	128:140	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	5	29	theme	stain	522:526	arg1	M1531T					528:533	stain M1531T	522:533	stain M1531T	522:533	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	5	30	theme	phylogenetic	469:480	arg1	analysis					482:489	phylogenetic analysis	469:489	phylogenetic analysis of the 16S rRNA gene sequence	469:519	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	1	31	theme	red	81:83	arg1	alga					85:88	a red alga	79:88	a red alga	79:88	nov., an alginate-utilizing bacterium isolated from a red alga.					
33774759	2	32	from	water	194:198	arg1	China					211:215	China	211:215	China	211:215	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	0	33	theme	Thalassotalea	0:12	arg1	sp					23:24	Thalassotalea algicola sp	0:24	Thalassotalea algicola sp.	0:25	Thalassotalea algicola sp.					
33774759	8	34	theme	predominant	930:940	arg1	quinone					953:959	the predominant isoprenoid quinone	926:959	the predominant isoprenoid quinone	926:959	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	34	theme	predominant	930:940	arg1	Q-8					919:921	Q-8	919:921	Q-8	919:921	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	35	theme	Strain	901:906	arg1	M1531T					908:913	Strain M1531T	901:913	Strain M1531T	901:913	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	5	36	theme	analysis	482:489	arg1	result					459:464	the result	455:464	the result of phylogenetic analysis of the 16S rRNA gene sequence	455:519	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	3	37	theme	polar	310:314	arg1	flagellum					316:324	a single polar flagellum	301:324	a single polar flagellum	301:324	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	7	38	theme	ANI	815:817	arg1	value					819:823	ANI value	815:823	ANI value	815:823	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	6	39	theme	4,061,950 bp	649:660	arg1	genes					716:720	four protein-coding genes	696:720	four protein-coding genes related to the degradation of alginate	696:759	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	6	39	theme	4,061,950 bp	649:660	arg1	size					641:644	a genome size	632:644	a genome size of 4,061,950 bp	632:660	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	6	39	theme	4,061,950 bp	649:660	arg1	content					671:677	a G + C content	663:677	a G + C content of 39.1 mol%	663:690	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	8	40	contain	possessed	965:973	arg2	acids					1085:1089	the major fatty acids	1069:1089	the major fatty acids	1069:1089	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	40	contain	possessed	965:973	arg2	Features					1033:1040	Summed Features	1026:1040	Summed Features	1026:1040	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	40	contain	possessed	965:973	arg2	Features					982:989	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	40	contain	possessed	965:973	arg2	C16:0					1016:1020	C16:0	1016:1020	C16:0	1016:1020	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	40	contain	possessed	965:973	arg1	M1531T					908:913	Strain M1531T	901:913	Strain M1531T	901:913	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	10	41	theme	novel	1431:1435	arg1	specie					1437:1442	a novel specie	1429:1442	a novel specie	1429:1442	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	10	42	theme	properties	1372:1381	arg1	results					1299:1305	the results	1295:1305	the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis	1295:1401	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	3	43	theme	novel	231:235	arg1	strain					237:242	the novel strain	227:242	the novel strain	227:242	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	2	44	theme	red	163:165	arg1	Porphyra					173:180	Porphyra	173:180	Porphyra	173:180	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	2	44	theme	red	163:165	arg1	alga					167:170	a red alga	161:170	a red alga (Porphyra)	161:181	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	12	45	theme	= MCCC	1563:1568	arg1	72865T					1586:1591	= MCCC 1H00400T = KCTC 72865T	1563:1591	= MCCC 1H00400T = KCTC 72865T	1563:1591	The type strain is M1531T (= MCCC 1H00400T = KCTC 72865T).					
33774759	12	45	theme	= MCCC	1563:1568	arg1	M1531T					1555:1560	M1531T	1555:1560	M1531T (= MCCC 1H00400T = KCTC 72865T)	1555:1592	The type strain is M1531T (= MCCC 1H00400T = KCTC 72865T).					
33774759	4	46	dep	%	407:407	arg1	w/v					410:412	w/v	410:412	w/v	410:412	Optimum growth occurred at 30 °C, with 2% (w/v) NaCl and at pH 6.5-7.0.					
33774759	8	47	theme	isoprenoid	942:951	arg1	quinone					953:959	the predominant isoprenoid quinone	926:959	the predominant isoprenoid quinone	926:959	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	47	theme	isoprenoid	942:951	arg1	Q-8					919:921	Q-8	919:921	Q-8	919:921	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	48	theme	fatty	1079:1083	arg1	Features					982:989	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	48	theme	fatty	1079:1083	arg1	acids					1085:1089	the major fatty acids	1069:1089	the major fatty acids	1069:1089	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	49	dep	C16:0	1016:1020	arg1	8					1042:1042	8	1042:1042	8	1042:1042	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	2	50	attach	isolated	147:154	arg2	M1531T					135:140	strain M1531T	128:140	strain M1531T	128:140	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	2	50	attach	isolated	147:154	arg1	Porphyra					173:180	Porphyra	173:180	Porphyra	173:180	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	2	50	attach	isolated	147:154	arg1	alga					167:170	a red alga	161:170	a red alga (Porphyra)	161:181	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	2	50	attach	isolated	147:154	arg2	bacterium					117:125	A facultatively anaerobic bacterium	91:125	A facultatively anaerobic bacterium	91:125	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	8	51	theme	Summed	1026:1031	arg1	Features					982:989	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	51	theme	Summed	1026:1031	arg1	Features					1033:1040	Summed Features	1026:1040	Summed Features	1026:1040	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	4	52	theme	2	406:406	arg1	%					407:407	%	407:407	%	407:407	Optimum growth occurred at 30 °C, with 2% (w/v) NaCl and at pH 6.5-7.0.					
33774759	5	53	dep	had	535:537	arg1	%					601:601	96.9%	597:601	96.9%	597:601	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	8	54	theme	major	1073:1077	arg1	Features					982:989	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	54	theme	major	1073:1077	arg1	acids					1085:1089	the major fatty acids	1069:1089	the major fatty acids	1069:1089	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	10	55	theme	Thalassotalea	1457:1469	arg1	specie					1437:1442	a novel specie	1429:1442	a novel specie	1429:1442	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	10	56	dep	phenotypic	1314:1323	arg1	chemotaxonomic					1326:1339	chemotaxonomic	1326:1339	chemotaxonomic	1326:1339	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	5	57	theme	Thalassotalea	559:571	arg1	KCTC					584:587	Thalassotalea euphylliae KCTC 42743T	559:594	Thalassotalea euphylliae KCTC 42743T	559:594	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	6	58	theme	related	722:728	arg1	genes					716:720	four protein-coding genes	696:720	four protein-coding genes related to the degradation of alginate	696:759	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	2	59	theme	coastal	186:192	arg1	water					194:198	coastal water	186:198	coastal water in Weihai, China	186:215	A facultatively anaerobic bacterium, strain M1531T, was isolated from a red alga (Porphyra) at coastal water in Weihai, China.					
33774759	9	60	theme	strain	1112:1117	arg1	M1531T					1119:1124	strain M1531T	1112:1124	strain M1531T	1112:1124	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	10	61	theme	phylogenetic	1359:1370	arg1	properties					1372:1381	phylogenetic properties	1359:1381	phylogenetic properties	1359:1381	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	10	62	theme	genus	1451:1455	arg1	Thalassotalea					1457:1469	the genus Thalassotalea	1447:1469	the genus Thalassotalea	1447:1469	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	5	63	theme	euphylliae	573:582	arg1	KCTC					584:587	Thalassotalea euphylliae KCTC 42743T	559:594	Thalassotalea euphylliae KCTC 42743T	559:594	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	6	64	theme	protein-coding	701:714	arg1	genes					716:720	four protein-coding genes	696:720	four protein-coding genes related to the degradation of alginate	696:759	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	6	65	theme	genome	634:639	arg1	size					641:644	a genome size	632:644	a genome size of 4,061,950 bp	632:660	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	7	66	theme	strain	794:799	arg1	M1531T					801:806	strain M1531T	794:806	strain M1531T	794:806	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	3	67	theme	strain	237:242	arg1	Cells					218:222	Cells	218:222	Cells of the novel strain	218:242	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	4	68	theme	Optimum	367:373	arg1	growth					375:380	Optimum growth	367:380	Optimum growth	367:380	Optimum growth occurred at 30 °C, with 2% (w/v) NaCl and at pH 6.5-7.0.					
33774759	6	69	theme	39.1 mol	682:689	arg1	%					690:690	39.1 mol%	682:690	39.1 mol%	682:690	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	12	70	theme	type	1540:1543	arg1	strain					1545:1550	The type strain	1536:1550	The type strain	1536:1550	The type strain is M1531T (= MCCC 1H00400T = KCTC 72865T).					
33774759	12	70	theme	type	1540:1543	arg1	M1531T					1555:1560	M1531T	1555:1560	M1531T (= MCCC 1H00400T = KCTC 72865T)	1555:1592	The type strain is M1531T (= MCCC 1H00400T = KCTC 72865T).					
33774759	9	71	theme	unidentified	1197:1208	arg1	phospholipid					1210:1221	one unidentified phospholipid	1193:1221	one unidentified phospholipid	1193:1221	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	9	71	theme	unidentified	1197:1208	arg1	lipids					1102:1107	The polar lipids	1092:1107	The polar lipids of strain M1531T	1092:1124	The polar lipids of strain M1531T were identified as phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and four unidentified lipids.					
33774759	10	72	theme	characterization	1341:1356	arg1	results					1299:1305	the results	1295:1305	the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis	1295:1401	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	7	73	theme	dDDH	839:842	arg1	%					836:836	95-96%	831:836	95-96%	831:836	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	7	73	theme	dDDH	839:842	arg1	value					844:848	dDDH value	839:848	dDDH value	839:848	According to the data obtained, strain M1531T shared ANI value below 95-96%, dDDH value below 23.8% with the closely related type species.					
33774759	12	74	theme	1H00400T = KCTC	1570:1584	arg1	72865T					1586:1591	= MCCC 1H00400T = KCTC 72865T	1563:1591	= MCCC 1H00400T = KCTC 72865T	1563:1591	The type strain is M1531T (= MCCC 1H00400T = KCTC 72865T).					
33774759	12	74	theme	1H00400T = KCTC	1570:1584	arg1	M1531T					1555:1560	M1531T	1555:1560	M1531T (= MCCC 1H00400T = KCTC 72865T)	1555:1592	The type strain is M1531T (= MCCC 1H00400T = KCTC 72865T).					
33774759	8	75	theme	Summed	975:980	arg1	Features					982:989	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Summed Features 3 (C16:1 ω7c/C16:1 ω6c)	975:1013	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	75	theme	Summed	975:980	arg1	C16:0					1016:1020	C16:0	1016:1020	C16:0	1016:1020	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	75	theme	Summed	975:980	arg1	acids					1085:1089	the major fatty acids	1069:1089	the major fatty acids	1069:1089	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	8	75	theme	Summed	975:980	arg1	Features					1033:1040	Summed Features	1026:1040	Summed Features	1026:1040	Strain M1531T had Q-8 as the predominant isoprenoid quinone and possessed Summed Features 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and Summed Features 8 (C18:1 ω7c/C18:1 ω6c) as the major fatty acids.					
33774759	10	76	theme	algicola	1505:1512	arg1	sp					1514:1515	the name Thalassotalea algicola sp	1482:1515	the name Thalassotalea algicola sp	1482:1515	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	5	77	with	relative	545:552	arg1	KCTC					584:587	Thalassotalea euphylliae KCTC 42743T	559:594	Thalassotalea euphylliae KCTC 42743T	559:594	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	10	78	theme	analysis	1394:1401	arg1	results					1299:1305	the results	1295:1305	the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis	1295:1401	According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain M1531T represents a novel specie of the genus Thalassotalea, for which the name Thalassotalea algicola sp.					
33774759	3	79	from	0.6-0.8 × 2.0-3.0 µm	337:356	arg1	size					361:364	size	361:364	size	361:364	Cells of the novel strain were Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum and around 0.6-0.8 × 2.0-3.0 µm in size.					
33774759	1	80	theme	alginate-utilizing	36:53	arg1	bacterium					55:63	an alginate-utilizing bacterium	33:63	an alginate-utilizing bacterium	33:63	nov., an alginate-utilizing bacterium isolated from a red alga.					
33774759	1	80	theme	alginate-utilizing	36:53	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., an alginate-utilizing bacterium isolated from a red alga.					
33774759	6	81	theme	Genome	605:610	arg1	sequencing					612:621	Genome sequencing	605:621	Genome sequencing	605:621	Genome sequencing revealed a genome size of 4,061,950 bp, a G + C content of 39.1 mol% and four protein-coding genes related to the degradation of alginate.					
33774759	5	82	contain	had	535:537	arg1	M1531T					528:533	stain M1531T	522:533	stain M1531T	522:533	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
33774759	5	82	contain	had	535:537	arg2	relative					545:552	close relative	539:552	close relative with Thalassotalea euphylliae KCTC 42743T	539:594	On the basis of the result of phylogenetic analysis of the 16S rRNA gene sequence, stain M1531T had close relative with Thalassotalea euphylliae KCTC 42743T (96.9%).					
34072653	4	0	theme	divalent	684:691	arg1	cations					693:699	divalent cations	684:699	divalent cations	684:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	5	1	dep	biopolymers	800:810	arg1	ALG					820:822	ALG	820:822	ALG	820:822	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	5	1	dep	biopolymers	800:810	arg1	SPI					812:814	SPI	812:814	SPI	812:814	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	5	1	dep	biopolymers	800:810	arg1	biopolymers					800:810	Both biopolymers SPI and ALG	795:822	Both biopolymers SPI and ALG	795:822	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	7	2	theme	matrices	1224:1231	arg1	properties					1189:1198	the mechanical properties	1174:1198	the mechanical properties of the resulting porous matrices	1174:1231	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	4	3	from	structure	669:677	arg1	present					644:650	present	644:650	present	644:650	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	1	4	theme	applications	262:273	arg1	range					253:257	a wide range	246:257	a wide range of applications	246:273	Composite materials based on proteins and carbohydrates normally offer improved water solubility, biodegradability, and biocompatibility, which make them attractive for a wide range of applications.					
34072653	8	5	theme	thanks	1360:1365	arg1	solubility					1349:1358	solubility	1349:1358	solubility thanks to the gelation of alginate	1349:1393	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	7	6	theme	different	1088:1096	arg1	solutions					1104:1112	different CaCl2 solutions	1088:1112	different CaCl2 solutions	1088:1112	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	5	7	theme	injection	864:872	arg1	moulding					874:881	injection moulding	864:881	injection moulding using glycerol (Gly) as a plasticizer	864:919	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	5	8	used	used	829:832	arg2	biopolymers					800:810	Both biopolymers SPI and ALG	795:822	Both biopolymers SPI and ALG	795:822	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	5	8	used	used	829:832	arg2	SPI					812:814	SPI	812:814	SPI	812:814	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	5	8	used	used	829:832	arg2	ALG					820:822	ALG	820:822	ALG	820:822	Both biopolymers SPI and ALG were used to produce composites through injection moulding using glycerol (Gly) as a plasticizer.					
34072653	7	9	theme	mechanical	1178:1187	arg1	properties					1189:1198	the mechanical properties	1174:1198	the mechanical properties of the resulting porous matrices	1174:1231	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	7	10	theme	CaCl2	1098:1102	arg1	solutions					1104:1112	different CaCl2 solutions	1088:1112	different CaCl2 solutions	1088:1112	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	2	11	from	useful	347:352	arg1	fields					357:362	fields	357:362	fields such as agriculture	357:382	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	2	11	from	useful	347:352	arg1	agriculture					372:382	agriculture	372:382	agriculture	372:382	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	7	12	theme	porous	1217:1222	arg1	matrices					1224:1231	the resulting porous matrices	1203:1231	the resulting porous matrices	1203:1231	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	1	13	theme	improved	148:155	arg1	solubility					163:172	improved water solubility	148:172	improved water solubility	148:172	Composite materials based on proteins and carbohydrates normally offer improved water solubility, biodegradability, and biocompatibility, which make them attractive for a wide range of applications.					
34072653	1	14	theme	Composite	77:85	arg1	materials					87:95	Composite materials	77:95	Composite materials based on proteins and carbohydrates	77:131	Composite materials based on proteins and carbohydrates normally offer improved water solubility, biodegradability, and biocompatibility, which make them attractive for a wide range of applications.					
34072653	4	15	theme	present	644:650	arg1	acid					639:642	guluronic acid	629:642	guluronic acid present in its molecular structure with divalent cations	629:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	15	theme	present	644:650	arg1	Ca2+					715:718	Ca2+	715:718	particularly Ca2+	702:718	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	16	with	structure	669:677	arg1	cations					693:699	divalent cations	684:699	divalent cations	684:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	2	17	from	fields	357:362	arg1	useful					347:352	useful	347:352	useful	347:352	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	2	18	theme	protein	280:286	arg1	SPI					297:299	SPI	297:299	SPI	297:299	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	2	18	theme	protein	280:286	arg1	isolate					288:294	Soy protein isolate	276:294	Soy protein isolate (SPI)	276:300	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	4	19	from	present	644:650	arg1	structure					669:677	its molecular structure	655:677	its molecular structure with divalent cations	655:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	3	20	theme	Alginate	385:392	arg1	polysaccharides					425:439	linear anionic polysaccharides	410:439	linear anionic polysaccharides obtained at a low cost from brown algae	410:479	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	3	20	theme	Alginate	385:392	arg1	ALG					401:403	ALG	401:403	ALG	401:403	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	3	20	theme	Alginate	385:392	arg1	salts					394:398	Alginate salts	385:398	Alginate salts (ALG)	385:404	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	2	21	theme	superabsorbent	312:325	arg1	properties					327:336	superabsorbent properties	312:336	superabsorbent properties that are useful in fields such as agriculture	312:382	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	4	22	from	acid	639:642	arg1	ions					619:622	ions	619:622	ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+,	619:719	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	22	from	acid	639:642	arg1	exchange					607:614	the exchange	603:614	the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+,	603:719	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	3	23	theme	linear	410:415	arg1	polysaccharides					425:439	linear anionic polysaccharides	410:439	linear anionic polysaccharides obtained at a low cost from brown algae	410:479	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	3	23	theme	linear	410:415	arg1	salts					394:398	Alginate salts	385:398	Alginate salts (ALG)	385:404	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	3	24	from	algae	475:479	arg1	cost					459:462	a low cost	453:462	a low cost from brown algae	453:479	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	3	25	theme	anionic	417:423	arg1	polysaccharides					425:439	linear anionic polysaccharides	410:439	linear anionic polysaccharides obtained at a low cost from brown algae	410:479	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	3	25	theme	anionic	417:423	arg1	salts					394:398	Alginate salts	385:398	Alginate salts (ALG)	385:404	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	0	26	theme	Absorption	19:28	arg1	Properties					30:39	Water Absorption Properties	13:39	Water Absorption Properties	13:39	Rheology and Water Absorption Properties of Alginate-Soy Protein Composites.					
34072653	3	27	theme	low	455:457	arg1	cost					459:462	a low cost	453:462	a low cost from brown algae	453:479	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	0	28	theme	Water	13:17	arg1	Properties					30:39	Water Absorption Properties	13:39	Water Absorption Properties	13:39	Rheology and Water Absorption Properties of Alginate-Soy Protein Composites.					
34072653	6	29	theme	biopolymer/plasticizer	932:953	arg1	ratios					955:960	Different biopolymer/plasticizer ratios	922:960	Different biopolymer/plasticizer ratios	922:960	Different biopolymer/plasticizer ratios were employed, and the SPI/ALG ratio within the biopolymer fraction was also varied.					
34072653	3	30	theme	medical	545:551	arg1	applications					553:564	medical applications	545:564	medical applications	545:564	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	8	31	theme	main	1238:1241	arg1	goal					1243:1246	The main goal	1234:1246	The main goal of the present work	1234:1266	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	8	31	theme	main	1238:1241	arg1	development					1276:1286	development	1276:1286	development	1276:1286	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	6	32	theme	Different	922:930	arg1	ratios					955:960	Different biopolymer/plasticizer ratios	922:960	Different biopolymer/plasticizer ratios	922:960	Different biopolymer/plasticizer ratios were employed, and the SPI/ALG ratio within the biopolymer fraction was also varied.					
34072653	4	33	attach	present	644:650	arg2	Ca2+					715:718	Ca2+	715:718	particularly Ca2+	702:718	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	33	attach	present	644:650	arg2	acid					639:642	guluronic acid	629:642	guluronic acid present in its molecular structure with divalent cations	629:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	33	attach	present	644:650	arg1	structure					669:677	its molecular structure	655:677	its molecular structure with divalent cations	655:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	34	theme	guluronic	629:637	arg1	acid					639:642	guluronic acid	629:642	guluronic acid present in its molecular structure with divalent cations	629:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	34	theme	guluronic	629:637	arg1	Ca2+					715:718	Ca2+	715:718	particularly Ca2+	702:718	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	7	35	theme	soluble	1139:1145	arg1	loss					1154:1157	soluble matter loss	1139:1157	soluble matter loss	1139:1157	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	6	36	theme	biopolymer	1010:1019	arg1	fraction					1021:1028	the biopolymer fraction	1006:1028	the biopolymer fraction	1006:1028	Different biopolymer/plasticizer ratios were employed, and the SPI/ALG ratio within the biopolymer fraction was also varied.					
34072653	0	37	theme	Protein	57:63	arg1	Composites					65:74	Alginate-Soy Protein Composites	44:74	Alginate-Soy Protein Composites	44:74	Rheology and Water Absorption Properties of Alginate-Soy Protein Composites.					
34072653	8	38	theme	porous	1318:1323	arg1	matrices					1325:1332	green porous matrices	1312:1332	green porous matrices	1312:1332	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	7	39	theme	resulting	1207:1215	arg1	matrices					1224:1231	the resulting porous matrices	1203:1231	the resulting porous matrices	1203:1231	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	8	40	theme	alginate	1386:1393	arg1	gelation					1374:1381	the gelation	1370:1381	the gelation of alginate	1370:1393	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	8	41	theme	work	1263:1266	arg1	goal					1243:1246	The main goal	1234:1246	The main goal of the present work	1234:1266	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	8	41	theme	work	1263:1266	arg1	development					1276:1286	development	1276:1286	development	1276:1286	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	0	42	theme	Alginate-Soy	44:55	arg1	Composites					65:74	Alginate-Soy Protein Composites	44:74	Alginate-Soy Protein Composites	44:74	Rheology and Water Absorption Properties of Alginate-Soy Protein Composites.					
34072653	8	43	dep	development	1276:1286	arg1	the					1272:1274	the	1272:1274	the	1272:1274	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	8	44	theme	present	1255:1261	arg1	work					1263:1266	the present work	1251:1266	the present work	1251:1266	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	2	45	theme	Soy	276:278	arg1	SPI					297:299	SPI	297:299	SPI	297:299	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	2	45	theme	Soy	276:278	arg1	isolate					288:294	Soy protein isolate	276:294	Soy protein isolate (SPI)	276:300	Soy protein isolate (SPI) has shown superabsorbent properties that are useful in fields such as agriculture.					
34072653	8	46	theme	matrices	1325:1332	arg1	goal					1243:1246	The main goal	1234:1246	The main goal of the present work	1234:1266	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	8	46	theme	matrices	1325:1332	arg1	characterization					1292:1307	characterization	1292:1307	characterization	1292:1307	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	8	46	theme	matrices	1325:1332	arg1	development					1276:1286	development	1276:1286	development	1276:1286	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	7	47	theme	loss	1154:1157	arg1	amount					1129:1134	the amount	1125:1134	the amount of soluble matter loss	1125:1157	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	7	47	theme	loss	1154:1157	arg1	loss					1154:1157	soluble matter loss	1139:1157	soluble matter loss	1139:1157	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	3	48	theme	brown	469:473	arg1	algae					475:479	brown algae	469:479	brown algae	469:479	Alginate salts (ALG) are linear anionic polysaccharides obtained at a low cost from brown algae, displaying a good enough biocompatibility to be considered for medical applications.					
34072653	8	49	theme	green	1312:1316	arg1	matrices					1325:1332	green porous matrices	1312:1332	green porous matrices	1312:1332	The main goal of the present work was the development and characterization of green porous matrices with inhibited solubility thanks to the gelation of alginate.					
34072653	4	50	from	exchange	607:614	arg1	acid					639:642	guluronic acid	629:642	guluronic acid present in its molecular structure with divalent cations	629:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	50	from	exchange	607:614	arg1	Ca2+					715:718	Ca2+	715:718	particularly Ca2+	702:718	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	4	51	theme	ions	619:622	arg1	exchange					607:614	the exchange	603:614	the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+,	603:719	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	1	52	theme	water	157:161	arg1	solubility					163:172	improved water solubility	148:172	improved water solubility	148:172	Composite materials based on proteins and carbohydrates normally offer improved water solubility, biodegradability, and biocompatibility, which make them attractive for a wide range of applications.					
34072653	1	53	theme	wide	248:251	arg1	range					253:257	a wide range	246:257	a wide range of applications	246:273	Composite materials based on proteins and carbohydrates normally offer improved water solubility, biodegradability, and biocompatibility, which make them attractive for a wide range of applications.					
34072653	0	54	theme	Composites	65:74	arg1	Rheology					0:7	Rheology	0:7	Rheology	0:7	Rheology and Water Absorption Properties of Alginate-Soy Protein Composites.					
34072653	0	54	theme	Composites	65:74	arg1	Properties					30:39	Water Absorption Properties	13:39	Water Absorption Properties	13:39	Rheology and Water Absorption Properties of Alginate-Soy Protein Composites.					
34072653	7	55	theme	matter	1147:1152	arg1	loss					1154:1157	soluble matter loss	1139:1157	soluble matter loss	1139:1157	Furthermore, composites were immersed in different CaCl2 solutions to inhibit the amount of soluble matter loss and to enhance the mechanical properties of the resulting porous matrices.					
34072653	4	56	theme	molecular	659:667	arg1	structure					669:677	its molecular structure	655:677	its molecular structure with divalent cations	655:699	As alginates are quite hydrophilic, the exchange of ions from guluronic acid present in its molecular structure with divalent cations, particularly Ca2+, may induce its gelation, which would inhibit its solubilization in water.					
34072653	6	57	theme	SPI/ALG	985:991	arg1	ratio					993:997	the SPI/ALG ratio	981:997	the SPI/ALG ratio within the biopolymer fraction	981:1028	Different biopolymer/plasticizer ratios were employed, and the SPI/ALG ratio within the biopolymer fraction was also varied.					
34817703	7	0	theme	chemical	1186:1193	arg1	composition					1195:1205	chemical composition	1186:1205	chemical composition	1186:1205	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	6	1	from	anode	1129:1133	arg1	reaction					1038:1045	the reaction	1034:1045	the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	1034:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	2	2	theme	antibacterial	407:419	arg1	functions					421:429	wide-spectrum antibacterial functions	393:429	wide-spectrum antibacterial functions	393:429	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	6	3	theme	signal	988:993	arg1	release					1006:1012	an electrical signal controlled release	974:1012	an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	974:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	12	4	theme	simple	1910:1915	arg1	platform					1917:1924	a general and simple platform	1896:1924	a general and simple platform to design alginate films with versatile functions	1896:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	11	5	theme	wound	1827:1831	arg1	treatment					1833:1841	infected wound treatment	1818:1841	infected wound treatment	1818:1841	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	5	6	theme	metal	760:764	arg1	ions					766:769	metal ions	760:769	metal ions	760:769	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	8	7	theme	In	1325:1326	arg1	tests					1334:1338	In vitro tests	1325:1338	In vitro tests	1325:1338	In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities.					
34817703	6	8	theme	films	892:896	arg1	fabrication					834:844	rapid fabrication	828:844	rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	828:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	1	9	with	contamination	125:137	arg1	bacteria					161:168	bacteria especially methicillin-resistant Staphylococcus aureus (MRSA)	161:230	bacteria especially methicillin-resistant Staphylococcus aureus (MRSA)	161:230	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	7	10	dep	structure	1172:1180	arg1	the					1159:1161	the	1159:1161	the	1159:1161	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	6	11	theme	alginate	869:876	arg1	Cu2+-Alg					899:906	Cu2+-Alg	899:906	Cu2+-Alg	899:906	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	11	theme	alginate	869:876	arg1	films					892:896	copper cross-linked alginate antibacterial films	849:896	copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	849:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	12	theme	insoluble	1050:1058	arg1	OH					1069:1070	OH	1069:1070	OH	1069:1070	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	12	theme	insoluble	1050:1058	arg1	Cu2					1065:1067	insoluble salt Cu2	1050:1067	insoluble salt Cu2(OH)2CO3	1050:1075	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	12	13	theme	general	1898:1904	arg1	platform					1917:1924	a general and simple platform	1896:1924	a general and simple platform to design alginate films with versatile functions	1896:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	1	14	theme	major	253:257	arg1	factor					259:264	the major factor	249:264	the major factor interferencing normal wound healing	249:300	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	1	14	theme	major	253:257	arg1	contamination					125:137	The contamination	121:137	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA)	121:230	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	4	15	theme	trace	513:517	arg1	elements					519:526	the trace elements	509:526	the trace elements in human body with inherent antibacterial activity	509:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	8	16	theme	content-dependent	1370:1386	arg1	activities					1401:1410	Cu2+ content-dependent bactericidal activities	1365:1410	Cu2+ content-dependent bactericidal activities	1365:1410	In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities.					
34817703	5	17	theme	copper-alginate	637:651	arg1	matrix					667:672	a structure-controlled copper-alginate antibacterial matrix	614:672	a structure-controlled copper-alginate antibacterial matrix	614:672	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	1	18	theme	wound	150:154	arg1	factor					259:264	the major factor	249:264	the major factor interferencing normal wound healing	249:300	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	1	18	theme	wound	150:154	arg1	contamination					125:137	The contamination	121:137	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA)	121:230	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	6	19	theme	copper	1017:1022	arg1	ions					1024:1027	copper ions	1017:1027	copper ions	1017:1027	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	5	20	theme	Traditional	580:590	arg1	methods					592:598	Traditional methods	580:598	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix	580:672	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	0	21	theme	controllable	15:26	arg1	electro-fabrication					28:46	Continuous and controllable electro-fabrication	0:46	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds	0:108	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	6	22	theme	2CO3	1072:1075	arg1	reaction					1038:1045	the reaction	1034:1045	the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	1034:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	4	23	theme	inherent	547:554	arg1	activity					570:577	inherent antibacterial activity	547:577	inherent antibacterial activity	547:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	6	24	from	reaction	1038:1045	arg1	anode					1129:1133	anode	1129:1133	anode	1129:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	24	from	reaction	1038:1045	arg1	release					1006:1012	an electrical signal controlled release	974:1012	an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	974:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	8	25	dep	film	1356:1359	arg1	has					1361:1363	has	1361:1363	has Cu2+ content-dependent bactericidal activities	1361:1410	In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities.					
34817703	6	26	theme	Cu2	1065:1067	arg1	2CO3					1072:1075	insoluble salt Cu2(OH)2CO3	1050:1075	insoluble salt Cu2(OH)2CO3	1050:1075	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	10	27	theme	Cu2+-Alg	1564:1571	arg1	film					1573:1576	Cu2+-Alg film	1564:1576	Cu2+-Alg film	1564:1576	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	0	28	theme	Continuous	0:9	arg1	electro-fabrication					28:46	Continuous and controllable electro-fabrication	0:46	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds	0:108	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	7	29	theme	films	1240:1244	arg1	structure					1172:1180	physical structure	1163:1180	physical structure	1163:1180	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	7	29	theme	films	1240:1244	arg1	composition					1195:1205	chemical composition	1186:1205	chemical composition	1186:1205	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	2	30	with	dressings	378:386	arg1	functions					421:429	wide-spectrum antibacterial functions	393:429	wide-spectrum antibacterial functions	393:429	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	6	31	theme	water	1107:1111	arg1	electrolysis					1113:1124	water electrolysis	1107:1124	water electrolysis	1107:1124	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	3	32	theme	wound	475:479	arg1	treatment					481:489	wound treatment	475:489	wound treatment	475:489	Alginate hydrogel is a common dressing for wound treatment.					
34817703	7	33	theme	electrodeposited	1214:1229	arg1	films					1240:1244	the electrodeposited Cu2+-Alg films	1210:1244	the electrodeposited Cu2+-Alg films	1210:1244	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	6	34	theme	protons	1095:1101	arg1	reaction					1038:1045	the reaction	1034:1045	the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	1034:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	10	35	theme	inflammatory	1642:1653	arg1	responses					1655:1663	host inflammatory responses	1637:1663	host inflammatory responses	1637:1663	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	2	36	theme	wide-spectrum	393:405	arg1	functions					421:429	wide-spectrum antibacterial functions	393:429	wide-spectrum antibacterial functions	393:429	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	0	37	theme	copper-alginate	65:79	arg1	dressing					81:88	antimicrobial copper-alginate dressing	51:88	antimicrobial copper-alginate dressing	51:88	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	6	38	theme	copper	939:944	arg1	content					946:952	copper content	939:952	copper content	939:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	39	from	protons	1095:1101	arg1	anode					1129:1133	anode	1129:1133	anode	1129:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	40	theme	rapid	828:832	arg1	fabrication					834:844	rapid fabrication	828:844	rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	828:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	9	41	theme	Cu2+-Alg	1484:1491	arg1	fabrication					1493:1503	Cu2+-Alg fabrication	1484:1503	Cu2+-Alg fabrication	1484:1503	Film's cytocompatibility is well controlled by the imposed charges for Cu2+-Alg fabrication.					
34817703	11	42	theme	controllable	1718:1729	arg1	strategy					1731:1738	a convenient and controllable strategy	1701:1738	a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment	1701:1841	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	11	43	theme	antibacterial	1762:1774	arg1	dressings					1776:1784	alginate antibacterial dressings	1753:1784	alginate antibacterial dressings	1753:1784	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	1	44	theme	normal	281:286	arg1	healing					294:300	normal wound healing	281:300	normal wound healing	281:300	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	12	45	theme	versatile	1956:1964	arg1	functions					1966:1974	versatile functions	1956:1974	versatile functions	1956:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	6	46	theme	controlled	995:1004	arg1	release					1006:1012	an electrical signal controlled release	974:1012	an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	974:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	10	47	theme	wound	1524:1528	arg1	model					1530:1534	The MRSA infected wound model	1506:1534	The MRSA infected wound model in vivo	1506:1542	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	7	48	theme	physical	1163:1170	arg1	structure					1172:1180	physical structure	1163:1180	physical structure	1163:1180	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	6	49	link	cross-linked	856:867	arg1	Cu2+-Alg					899:906	Cu2+-Alg	899:906	Cu2+-Alg	899:906	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	49	link	cross-linked	856:867	arg1	films					892:896	copper cross-linked alginate antibacterial films	849:896	copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	849:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	50	theme	electrical	977:986	arg1	release					1006:1012	an electrical signal controlled release	974:1012	an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	974:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	12	51	theme	design	1929:1934	arg1	films					1945:1949	design alginate films	1929:1949	design alginate films with versatile functions	1929:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	12	52	dep	electrodeposition	1875:1891	arg1	platform					1917:1924	a general and simple platform	1896:1924	a general and simple platform to design alginate films with versatile functions	1896:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	5	53	theme	fast	708:711	arg1	gelation					730:737	the fast and uncontrolled gelation	704:737	the fast and uncontrolled gelation between alginate and metal ions	704:769	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	2	54	theme	great	322:326	arg1	challenges					328:337	great challenges	322:337	great challenges	322:337	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	11	55	theme	infected	1818:1825	arg1	treatment					1833:1841	infected wound treatment	1818:1841	infected wound treatment	1818:1841	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	5	56	theme	uncontrolled	717:728	arg1	gelation					730:737	the fast and uncontrolled gelation	704:737	the fast and uncontrolled gelation between alginate and metal ions	704:769	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	6	57	theme	antibacterial	878:890	arg1	Cu2+-Alg					899:906	Cu2+-Alg	899:906	Cu2+-Alg	899:906	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	57	theme	antibacterial	878:890	arg1	films					892:896	copper cross-linked alginate antibacterial films	849:896	copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	849:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	2	58	theme	effective	362:370	arg1	dressings					378:386	safe and effective wound dressings	353:386	safe and effective wound dressings with wide-spectrum antibacterial functions	353:429	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	6	59	theme	salt	1060:1063	arg1	OH					1069:1070	OH	1069:1070	OH	1069:1070	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	59	theme	salt	1060:1063	arg1	Cu2					1065:1067	insoluble salt Cu2	1050:1067	insoluble salt Cu2(OH)2CO3	1050:1075	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	10	60	theme	infected	1515:1522	arg1	model					1530:1534	The MRSA infected wound model	1506:1534	The MRSA infected wound model in vivo	1506:1542	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	4	61	from	elements	519:526	arg1	body					537:540	human body	531:540	human body with inherent antibacterial activity	531:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	6	62	theme	cross-linked	856:867	arg1	Cu2+-Alg					899:906	Cu2+-Alg	899:906	Cu2+-Alg	899:906	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	62	theme	cross-linked	856:867	arg1	films					892:896	copper cross-linked alginate antibacterial films	849:896	copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	849:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	2	63	theme	safe	353:356	arg1	dressings					378:386	safe and effective wound dressings	353:386	safe and effective wound dressings with wide-spectrum antibacterial functions	353:429	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	12	64	with	films	1945:1949	arg1	functions					1966:1974	versatile functions	1956:1974	versatile functions	1956:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	4	65	theme	elements	519:526	arg1	one					502:504	one	502:504	one	502:504	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	4	65	theme	elements	519:526	arg1	elements					519:526	the trace elements	509:526	the trace elements in human body with inherent antibacterial activity	509:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	6	66	with	films	892:896	arg1	structure					925:933	controlled structure	914:933	controlled structure	914:933	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	66	with	films	892:896	arg1	content					946:952	copper content	939:952	copper content	939:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	5	67	theme	structure-controlled	616:635	arg1	matrix					667:672	a structure-controlled copper-alginate antibacterial matrix	614:672	a structure-controlled copper-alginate antibacterial matrix	614:672	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	1	68	theme	chronic	142:148	arg1	wound					150:154	chronic wound	142:154	chronic wound	142:154	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	6	69	theme	ions	1024:1027	arg1	release					1006:1012	an electrical signal controlled release	974:1012	an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode	974:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	8	70	theme	Cu2+	1365:1368	arg1	activities					1401:1410	Cu2+ content-dependent bactericidal activities	1365:1410	Cu2+ content-dependent bactericidal activities	1365:1410	In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities.					
34817703	10	71	theme	MRSA	1510:1513	arg1	model					1530:1534	The MRSA infected wound model	1506:1534	The MRSA infected wound model in vivo	1506:1542	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	5	72	theme	antibacterial	653:665	arg1	matrix					667:672	a structure-controlled copper-alginate antibacterial matrix	614:672	a structure-controlled copper-alginate antibacterial matrix	614:672	Traditional methods for preparing a structure-controlled copper-alginate antibacterial matrix are difficult however, due to the fast and uncontrolled gelation between alginate and metal ions.					
34817703	6	73	theme	generated	1085:1093	arg1	protons					1095:1101	the generated protons	1081:1101	the generated protons via water electrolysis on anode	1081:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	8	74	theme	bactericidal	1388:1399	arg1	activities					1401:1410	Cu2+ content-dependent bactericidal activities	1365:1410	Cu2+ content-dependent bactericidal activities	1365:1410	In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities.					
34817703	0	75	theme	infected	94:101	arg1	wounds					103:108	infected wounds	94:108	infected wounds	94:108	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	4	76	theme	antibacterial	556:568	arg1	activity					570:577	inherent antibacterial activity	547:577	inherent antibacterial activity	547:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	7	77	theme	imposed	1283:1289	arg1	charges					1291:1297	the imposed charges	1279:1297	the imposed charges	1279:1297	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	6	78	from	2CO3	1072:1075	arg1	anode					1129:1133	anode	1129:1133	anode	1129:1133	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	3	79	theme	Alginate	432:439	arg1	hydrogel					441:448	Alginate hydrogel	432:448	Alginate hydrogel	432:448	Alginate hydrogel is a common dressing for wound treatment.					
34817703	3	79	theme	Alginate	432:439	arg1	dressing					462:469	a common dressing	453:469	a common dressing for wound treatment	453:489	Alginate hydrogel is a common dressing for wound treatment.					
34817703	4	80	theme	human	531:535	arg1	body					537:540	human body	531:540	human body with inherent antibacterial activity	531:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	1	81	dep	bacteria	161:168	arg1	MRSA					226:229	MRSA	226:229	MRSA	226:229	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	1	81	dep	bacteria	161:168	arg1	aureus					218:223	Staphylococcus aureus	203:223	bacteria especially methicillin-resistant Staphylococcus aureus (MRSA)	161:230	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
34817703	0	82	dep	treatment	110:118	arg1	electro-fabrication					28:46	Continuous and controllable electro-fabrication	0:46	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds	0:108	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	3	83	theme	common	455:460	arg1	hydrogel					441:448	Alginate hydrogel	432:448	Alginate hydrogel	432:448	Alginate hydrogel is a common dressing for wound treatment.					
34817703	3	83	theme	common	455:460	arg1	dressing					462:469	a common dressing	453:469	a common dressing for wound treatment	453:489	Alginate hydrogel is a common dressing for wound treatment.					
34817703	7	84	theme	Cu2+-Alg	1231:1238	arg1	films					1240:1244	the electrodeposited Cu2+-Alg films	1210:1244	the electrodeposited Cu2+-Alg films	1210:1244	The results prove that the physical structure and chemical composition of the electrodeposited Cu2+-Alg films can be continuously modulated by the imposed charges during electrodeposition.					
34817703	8	85	dep	In	1325:1326	arg1	vitro					1328:1332	vitro	1328:1332	vitro	1328:1332	In vitro tests demonstrate the film has Cu2+ content-dependent bactericidal activities.					
34817703	10	86	theme	bacterial	1604:1612	arg1	infection					1614:1622	bacterial infection	1604:1622	bacterial infection	1604:1622	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	0	87	theme	antimicrobial	51:63	arg1	dressing					81:88	antimicrobial copper-alginate dressing	51:88	antimicrobial copper-alginate dressing	51:88	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	6	88	theme	copper	849:854	arg1	Cu2+-Alg					899:906	Cu2+-Alg	899:906	Cu2+-Alg	899:906	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	6	88	theme	copper	849:854	arg1	films					892:896	copper cross-linked alginate antibacterial films	849:896	copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content	849:952	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	0	89	theme	dressing	81:88	arg1	electro-fabrication					28:46	Continuous and controllable electro-fabrication	0:46	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds	0:108	Continuous and controllable electro-fabrication of antimicrobial copper-alginate dressing for infected wounds treatment.					
34817703	9	90	theme	imposed	1464:1470	arg1	charges					1472:1478	the imposed charges	1460:1478	the imposed charges for Cu2+-Alg fabrication	1460:1503	Film's cytocompatibility is well controlled by the imposed charges for Cu2+-Alg fabrication.					
34817703	10	91	theme	host	1637:1640	arg1	responses					1655:1663	host inflammatory responses	1637:1663	host inflammatory responses	1637:1663	The MRSA infected wound model in vivo also indicates that Cu2+-Alg film can effectively eliminate bacterial infection and suppress host inflammatory responses.					
34817703	11	92	theme	convenient	1703:1712	arg1	strategy					1731:1738	a convenient and controllable strategy	1701:1738	a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment	1701:1841	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	11	93	theme	alginate	1753:1760	arg1	dressings					1776:1784	alginate antibacterial dressings	1753:1784	alginate antibacterial dressings	1753:1784	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	2	94	theme	wound	372:376	arg1	dressings					378:386	safe and effective wound dressings	353:386	safe and effective wound dressings with wide-spectrum antibacterial functions	353:429	There still remain great challenges in developing safe and effective wound dressings with wide-spectrum antibacterial functions.					
34817703	6	95	theme	controlled	914:923	arg1	structure					925:933	controlled structure	914:933	controlled structure	914:933	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	12	96	theme	alginate	1936:1943	arg1	films					1945:1949	design alginate films	1929:1949	design alginate films with versatile functions	1929:1974	More broadly, our work reveals electrodeposition is a general and simple platform to design alginate films with versatile functions.					
34817703	11	97	theme	potential	1791:1799	arg1	applications					1801:1812	potential applications	1791:1812	potential applications for infected wound treatment	1791:1841	We believe this study demonstrates a convenient and controllable strategy to fabricate alginate antibacterial dressings with potential applications for infected wound treatment.					
34817703	6	98	theme	electrodeposition	799:815	arg1	method					817:822	an electrodeposition method	796:822	an electrodeposition method	796:822	In this work, we report an electrodeposition method for rapid fabrication of copper cross-linked alginate antibacterial films (Cu2+-Alg) with controlled structure and copper content, which is relied on an electrical signal controlled release of copper ions from the reaction of insoluble salt Cu2(OH)2CO3 and the generated protons via water electrolysis on anode.					
34817703	4	99	with	body	537:540	arg1	activity					570:577	inherent antibacterial activity	547:577	inherent antibacterial activity	547:577	Copper is one of the trace elements in human body with inherent antibacterial activity.					
34817703	1	100	theme	wound	288:292	arg1	healing					294:300	normal wound healing	281:300	normal wound healing	281:300	The contamination of chronic wound with bacteria especially methicillin-resistant Staphylococcus aureus (MRSA) is considered as the major factor interferencing normal wound healing.					
33361571	2	0	theme	extracellular	327:339	arg1	matrix					341:346	the extracellular matrix	323:346	the extracellular matrix (ECM)	323:352	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	2	0	theme	extracellular	327:339	arg1	ECM					349:351	ECM	349:351	ECM	349:351	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	2	1	theme	similar	262:268	arg1	structure					270:278	the similar structure	258:278	the similar structure	258:278	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	4	2	theme	mechanical	616:625	arg1	properties					627:636	mechanical properties	616:636	mechanical properties	616:636	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	3	3	theme	surface	503:509	arg1	area					511:514	large specific surface area	488:514	large specific surface area	488:514	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	8	4	from	surface	1380:1386	arg1	structure					1438:1446	a porous honeycomb structure	1419:1446	a porous honeycomb structure	1419:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	5	5	theme	simulated	836:844	arg1	1.5SBF					858:863	1.5SBF	858:863	1.5SBF	858:863	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	5	5	theme	simulated	836:844	arg1	fluid					851:855	1.5× simulated body fluid	831:855	1.5× simulated body fluid (1.5SBF)	831:864	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	5	6	theme	surface	1047:1053	arg1	modification					1017:1028	a prior chemical modification	1000:1028	a prior chemical modification of the substrate surface	1000:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	1	7	from	stability	208:216	arg1	fluid					232:236	human body fluid	221:236	human body fluid	221:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	2	8	with	Chitosan	239:246	arg1	structure					270:278	the similar structure	258:278	the similar structure	258:278	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	8	9	theme	fibrous	1398:1404	arg1	scaffolds					1406:1414	PA6/CS fibrous scaffolds	1391:1414	PA6/CS fibrous scaffolds in a porous honeycomb structure	1391:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	5	10	theme	prior	1002:1006	arg1	modification					1017:1028	a prior chemical modification	1000:1028	a prior chemical modification of the substrate surface	1000:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	3	11	theme	biological	548:557	arg1	function					559:566	biological function	548:566	biological function	548:566	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	1	12	theme	synthetic	121:129	arg1	polymer					131:137	a synthetic polymer	119:137	a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid	119:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	1	12	theme	synthetic	121:129	arg1	Polyamide-6					98:108	Polyamide-6	98:108	Polyamide-6 (PA6)	98:114	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	9	13	theme	MC3T3-E1	1659:1666	arg1	cell					1668:1671	MC3T3-E1 cell attachment and proliferation	1659:1700	cell	1668:1671	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	4	14	theme	PA6/CS	699:704	arg1	scaffolds					706:714	electrospun PA6/CS scaffolds	687:714	electrospun PA6/CS scaffolds	687:714	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	5	15	theme	modification	1017:1028	arg1	need					992:995	the need	988:995	the need of a prior chemical modification of the substrate surface	988:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	8	16	theme	porous	1421:1426	arg1	structure					1438:1446	a porous honeycomb structure	1419:1446	a porous honeycomb structure	1419:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	7	17	theme	±	1290:1290	arg1	MPa					1297:1299	95.52 ± 6.78 MPa	1284:1299	95.52 ± 6.78 MPa	1284:1299	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	7	18	theme	tensile	1192:1198	arg1	strength					1200:1207	tensile strength	1192:1207	tensile strength	1192:1207	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	0	19	theme	polyamide-6/chitosan	19:38	arg1	scaffold					40:47	electrospun polyamide-6/chitosan scaffold	7:47	electrospun polyamide-6/chitosan scaffold	7:47	Coated electrospun polyamide-6/chitosan scaffold with hydroxyapatite for bone tissue engineering.					
33361571	4	20	theme	tissue	667:672	arg1	engineering					674:684	bone tissue engineering	662:684	bone tissue engineering	662:684	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	5	21	theme	hydroxyapatite	885:898	arg1	layer					905:909	the hydroxyapatite (HA) layer	881:909	the hydroxyapatite (HA) layer	881:909	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	9	22	dep	cell	1668:1671	arg1	attachment					1673:1682	attachment	1673:1682	attachment	1673:1682	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	5	23	from	immersion	818:826	arg1	1.5SBF					858:863	1.5SBF	858:863	1.5SBF	858:863	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	5	23	from	immersion	818:826	arg1	fluid					851:855	1.5× simulated body fluid	831:855	1.5× simulated body fluid (1.5SBF)	831:864	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	3	24	contain	have	444:447	arg2	porosity					475:482	high porosity	470:482	high porosity	470:482	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	3	24	contain	have	444:447	arg2	structure					459:467	nanoscale structure	449:467	nanoscale structure	449:467	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	3	24	contain	have	444:447	arg2	area					511:514	large specific surface area	488:514	large specific surface area	488:514	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	3	24	contain	have	444:447	arg1	fiber					438:442	Electrospun fiber	426:442	Electrospun fiber	426:442	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	8	25	theme	mineral	1468:1474	arg1	%					1488:1488	about 40%	1480:1488	about 40%	1480:1488	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	8	25	theme	mineral	1468:1474	arg1	content					1457:1463	the content	1453:1463	the content of mineral	1453:1474	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	3	26	theme	Electrospun	426:436	arg1	fiber					438:442	Electrospun fiber	426:442	Electrospun fiber	426:442	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	8	27	from	structure	1438:1446	arg1	surface					1380:1386	the surface	1376:1386	the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure	1376:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	9	28	theme	cell	1504:1507	arg1	study					1517:1521	cell culture study	1504:1521	cell culture study	1504:1521	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	2	29	theme	suitable	366:373	arg1	rate					390:393	a more suitable biodegradation rate	359:393	a more suitable biodegradation rate for the formation of new-bone	359:423	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	8	30	theme	HA	1338:1339	arg1	particles					1341:1349	HA particles	1338:1349	HA particles	1338:1349	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	9	31	contain	had	1605:1607	arg1	non-cytotoxic					1586:1598	non-cytotoxic	1586:1598	non-cytotoxic	1586:1598	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	9	31	contain	had	1605:1607	arg2	biocompatibility					1616:1631	a good biocompatibility	1609:1631	a good biocompatibility	1609:1631	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	9	31	contain	had	1605:1607	arg1	scaffolds					1571:1579	the mineralized PA6/CS composite scaffolds	1538:1579	the mineralized PA6/CS composite scaffolds	1538:1579	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	9	31	contain	had	1605:1607	arg2	ability					1640:1646	an ability	1637:1646	an ability to promote MC3T3-E1 cell attachment and proliferation	1637:1700	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	5	32	theme	PA6/CS	779:784	arg1	scaffolds					786:794	The mineralized PA6/CS scaffolds	763:794	The mineralized PA6/CS scaffolds	763:794	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	0	33	theme	tissue	78:83	arg1	engineering					85:95	bone tissue engineering	73:95	bone tissue engineering	73:95	Coated electrospun polyamide-6/chitosan scaffold with hydroxyapatite for bone tissue engineering.					
33361571	6	34	theme	fibrous	1099:1105	arg1	scaffolds					1107:1115	electrospun PA6/CS fibrous scaffolds	1080:1115	electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm	1080:1150	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	3	35	theme	natural	575:581	arg1	ECM					583:585	the natural ECM	571:585	the natural ECM	571:585	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	1	36	theme	human	221:225	arg1	fluid					232:236	human body fluid	221:236	human body fluid	221:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	5	37	theme	reaction	960:967	arg1	conditions					969:978	very mild reaction conditions	950:978	very mild reaction conditions without the need of a prior chemical modification of the substrate surface	950:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	7	38	theme	fibrous	1233:1239	arg1	scaffolds					1241:1249	10PA6/CS fibrous scaffolds	1224:1249	10PA6/CS fibrous scaffolds	1224:1249	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	1	39	contain	has	194:196	arg1	polymer					131:137	a synthetic polymer	119:137	a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid	119:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	1	39	contain	has	194:196	arg2	stability					208:216	excellent stability	198:216	excellent stability in human body fluid	198:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	1	39	contain	has	194:196	arg1	Polyamide-6					98:108	Polyamide-6	98:108	Polyamide-6 (PA6)	98:114	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	7	40	dep	±	1269:1269	arg1	to					1260:1261	to	1260:1261	to	1260:1261	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	3	41	theme	high	470:473	arg1	porosity					475:482	high porosity	470:482	high porosity	470:482	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	6	42	theme	nm	1149:1150	arg1	range					1133:1137	the diameter range	1120:1137	the diameter range of 60-260 nm	1120:1150	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	3	43	theme	specific	494:501	arg1	area					511:514	large specific surface area	488:514	large specific surface area	488:514	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	1	44	contain	bears	144:148	arg1	polymer					131:137	a synthetic polymer	119:137	a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid	119:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	1	44	contain	bears	144:148	arg2	resemblance					150:160	resemblance	150:160	resemblance to collagen	150:172	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	1	44	contain	bears	144:148	arg1	Polyamide-6					98:108	Polyamide-6	98:108	Polyamide-6 (PA6)	98:114	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	1	44	contain	bears	144:148	arg3	backbone					181:188	its backbone	177:188	its backbone	177:188	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	9	45	theme	good	1611:1614	arg1	biocompatibility					1616:1631	a good biocompatibility	1609:1631	a good biocompatibility	1609:1631	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	6	46	theme	diameter	1124:1131	arg1	range					1133:1137	the diameter range	1120:1137	the diameter range of 60-260 nm	1120:1150	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	4	47	theme	properties	627:636	arg1	requirements					600:611	the requirements	596:611	the requirements of mechanical properties and biocompatibility of bone tissue engineering	596:684	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	5	48	theme	1.5×	831:834	arg1	1.5SBF					858:863	1.5SBF	858:863	1.5SBF	858:863	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	5	48	theme	1.5×	831:834	arg1	fluid					851:855	1.5× simulated body fluid	831:855	1.5× simulated body fluid (1.5SBF)	831:864	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	8	49	theme	PA6/CS	1391:1396	arg1	scaffolds					1406:1414	PA6/CS fibrous scaffolds	1391:1414	PA6/CS fibrous scaffolds in a porous honeycomb structure	1391:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	3	50	dep	structure	534:542	arg1	the					530:532	the	530:532	the	530:532	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	5	51	theme	substrate	1037:1045	arg1	surface					1047:1053	the substrate surface	1033:1053	the substrate surface	1033:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	5	52	theme	body	846:849	arg1	1.5SBF					858:863	1.5SBF	858:863	1.5SBF	858:863	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	5	52	theme	body	846:849	arg1	fluid					851:855	1.5× simulated body fluid	831:855	1.5× simulated body fluid (1.5SBF)	831:864	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	8	53	theme	scaffolds	1406:1414	arg1	surface					1380:1386	the surface	1376:1386	the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure	1376:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	6	54	theme	ECM	1183:1185	arg1	nanostructure					1162:1174	the nanostructure	1158:1174	the nanostructure of the ECM	1158:1185	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	4	55	theme	engineering	674:684	arg1	biocompatibility					642:657	biocompatibility	642:657	biocompatibility of bone tissue engineering	642:684	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	4	55	theme	engineering	674:684	arg1	properties					627:636	mechanical properties	616:636	mechanical properties	616:636	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	5	56	theme	chemical	1008:1015	arg1	modification					1017:1028	a prior chemical modification	1000:1028	a prior chemical modification of the substrate surface	1000:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	3	57	theme	ECM	583:585	arg1	function					559:566	biological function	548:566	biological function	548:566	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	3	57	theme	ECM	583:585	arg1	structure					534:542	structure	534:542	structure	534:542	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	8	58	theme	honeycomb	1428:1436	arg1	structure					1438:1446	a porous honeycomb structure	1419:1446	a porous honeycomb structure	1419:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	5	59	theme	thickness	928:936	arg1	range					938:942	the thickness range	924:942	the thickness range	924:942	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	4	60	theme	electrospun	687:697	arg1	scaffolds					706:714	electrospun PA6/CS scaffolds	687:714	electrospun PA6/CS scaffolds	687:714	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	6	61	theme	PA6/CS	1092:1097	arg1	scaffolds					1107:1115	electrospun PA6/CS fibrous scaffolds	1080:1115	electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm	1080:1150	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	8	62	from	scaffolds	1406:1414	arg1	structure					1438:1446	a porous honeycomb structure	1419:1446	a porous honeycomb structure	1419:1446	After mineralization, HA particles uniformly distributed on the surface of PA6/CS fibrous scaffolds in a porous honeycomb structure, and the content of mineral was about 40%.					
33361571	4	63	theme	biocompatibility	642:657	arg1	requirements					600:611	the requirements	596:611	the requirements of mechanical properties and biocompatibility of bone tissue engineering	596:684	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	4	64	theme	bone	662:665	arg1	engineering					674:684	bone tissue engineering	662:684	bone tissue engineering	662:684	To meet the requirements of mechanical properties and biocompatibility of bone tissue engineering, electrospun PA6/CS scaffolds were fabricated by electrospinning technology.					
33361571	9	65	theme	mineralized	1542:1552	arg1	non-cytotoxic					1586:1598	non-cytotoxic	1586:1598	non-cytotoxic	1586:1598	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	9	65	theme	mineralized	1542:1552	arg1	scaffolds					1571:1579	the mineralized PA6/CS composite scaffolds	1538:1579	the mineralized PA6/CS composite scaffolds	1538:1579	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	3	66	dep	have	444:447	arg1	simulate					521:528	simulate	521:528	can simulate the structure and biological function of the natural ECM	517:585	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	6	67	theme	electrospun	1080:1090	arg1	scaffolds					1107:1115	electrospun PA6/CS fibrous scaffolds	1080:1115	electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm	1080:1150	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	1	68	theme	excellent	198:206	arg1	stability					208:216	excellent stability	198:216	excellent stability in human body fluid	198:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	0	69	theme	electrospun	7:17	arg1	scaffold					40:47	electrospun polyamide-6/chitosan scaffold	7:47	electrospun polyamide-6/chitosan scaffold	7:47	Coated electrospun polyamide-6/chitosan scaffold with hydroxyapatite for bone tissue engineering.					
33361571	7	70	dep	strength	1200:1207	arg1	The					1188:1190	The	1188:1190	The	1188:1190	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	7	71	theme	2.31	1271:1274	arg1	±					1269:1269	±	1269:1269	±	1269:1269	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	7	72	theme	12.67	1263:1267	arg1	±					1269:1269	±	1269:1269	±	1269:1269	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	2	73	theme	biodegradation	375:388	arg1	rate					390:393	a more suitable biodegradation rate	359:393	a more suitable biodegradation rate for the formation of new-bone	359:423	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	5	74	theme	mineralized	767:777	arg1	scaffolds					786:794	The mineralized PA6/CS scaffolds	763:794	The mineralized PA6/CS scaffolds	763:794	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	9	75	theme	culture	1509:1515	arg1	study					1517:1521	cell culture study	1504:1521	cell culture study	1504:1521	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	0	76	theme	bone	73:76	arg1	engineering					85:95	bone tissue engineering	73:95	bone tissue engineering	73:95	Coated electrospun polyamide-6/chitosan scaffold with hydroxyapatite for bone tissue engineering.					
33361571	7	77	theme	scaffolds	1241:1249	arg1	modulus					1213:1219	modulus	1213:1219	modulus	1213:1219	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	7	77	theme	scaffolds	1241:1249	arg1	strength					1200:1207	tensile strength	1192:1207	tensile strength	1192:1207	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	2	78	theme	new-bone	416:423	arg1	formation					403:411	the formation	399:411	the formation of new-bone	399:423	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	6	79	from	scaffolds	1107:1115	arg1	range					1133:1137	the diameter range	1120:1137	the diameter range of 60-260 nm	1120:1150	The results showed that electrospun PA6/CS fibrous scaffolds in the diameter range of 60-260 nm mimic the nanostructure of the ECM.					
33361571	2	80	contain	has	355:357	arg2	rate					390:393	a more suitable biodegradation rate	359:393	a more suitable biodegradation rate for the formation of new-bone	359:423	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	2	80	contain	has	355:357	arg1	CS					249:250	CS	249:250	CS	249:250	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	2	80	contain	has	355:357	arg1	Chitosan					239:246	Chitosan	239:246	Chitosan (CS)	239:251	Chitosan (CS) with the similar structure to that of the polysaccharides existing in the extracellular matrix (ECM), has a more suitable biodegradation rate for the formation of new-bone.					
33361571	9	81	theme	composite	1561:1569	arg1	non-cytotoxic					1586:1598	non-cytotoxic	1586:1598	non-cytotoxic	1586:1598	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	9	81	theme	composite	1561:1569	arg1	scaffolds					1571:1579	the mineralized PA6/CS composite scaffolds	1538:1579	the mineralized PA6/CS composite scaffolds	1538:1579	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	5	82	theme	mild	955:958	arg1	conditions					969:978	very mild reaction conditions	950:978	very mild reaction conditions without the need of a prior chemical modification of the substrate surface	950:1053	The mineralized PA6/CS scaffolds were obtained through immersion in 1.5× simulated body fluid (1.5SBF), which allowed the hydroxyapatite (HA) layer to grow into the thickness range under very mild reaction conditions without the need of a prior chemical modification of the substrate surface.					
33361571	7	83	theme	10PA6/CS	1224:1231	arg1	scaffolds					1241:1249	10PA6/CS fibrous scaffolds	1224:1249	10PA6/CS fibrous scaffolds	1224:1249	The tensile strength and modulus of 10PA6/CS fibrous scaffolds reach up to 12.67 ± 2.31 MPa and 95.52 ± 6.78 MPa, respectively.					
33361571	3	84	theme	nanoscale	449:457	arg1	structure					459:467	nanoscale structure	449:467	nanoscale structure	449:467	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
33361571	1	85	theme	body	227:230	arg1	fluid					232:236	human body fluid	221:236	human body fluid	221:236	Polyamide-6 (PA6) is a synthetic polymer that bears resemblance to collagen in its backbone and has excellent stability in human body fluid.					
33361571	9	86	theme	PA6/CS	1554:1559	arg1	non-cytotoxic					1586:1598	non-cytotoxic	1586:1598	non-cytotoxic	1586:1598	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	9	86	theme	PA6/CS	1554:1559	arg1	scaffolds					1571:1579	the mineralized PA6/CS composite scaffolds	1538:1579	the mineralized PA6/CS composite scaffolds	1538:1579	In addition, cell culture study indicated that the mineralized PA6/CS composite scaffolds were non-cytotoxic, and had a good biocompatibility and an ability to promote MC3T3-E1 cell attachment and proliferation.					
33361571	3	87	theme	large	488:492	arg1	area					511:514	large specific surface area	488:514	large specific surface area	488:514	Electrospun fiber have nanoscale structure, high porosity and large specific surface area, can simulate the structure and biological function of the natural ECM.					
34626727	0	0	theme	clove	100:104	arg1	oil					116:118	nanoencapsulated clove essential oil	83:118	nanoencapsulated clove essential oil	83:118	Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.					
34626727	7	1	theme	CEO	1070:1072	arg1	property					1058:1065	a controlled-release property	1037:1065	a controlled-release property of CEO	1037:1072	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	2	2	theme	sodium	347:352	arg1	NaCas					365:369	NaCas	365:369	NaCas	365:369	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	2	2	theme	sodium	347:352	arg1	caseinate					354:362	sodium caseinate	347:362	sodium caseinate (NaCas)	347:370	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	6	3	theme	intermolecular	924:937	arg1	bonds					948:952	the intermolecular hydrogen bonds	920:952	the intermolecular hydrogen bonds	920:952	The infrared spectroscopy verified that the intermolecular hydrogen bonds exist between chitosan and C/ZN nanoparticles.					
34626727	0	4	theme	nanoencapsulated	83:98	arg1	oil					116:118	nanoencapsulated clove essential oil	83:118	nanoencapsulated clove essential oil	83:118	Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.					
34626727	2	5	contain	containing	265:274	arg2	nanoparticles					309:321	clove essential oil (CEO)-loaded nanoparticles	276:321	clove essential oil (CEO)-loaded nanoparticles	276:321	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	2	5	contain	containing	265:274	arg1	emulsion					256:263	a Pickering emulsion	244:263	a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas)	244:370	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	7	6	theme	antibacterial	1141:1153	arg1	property					1155:1162	the improved antibacterial property	1128:1162	the improved antibacterial property	1128:1162	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	5	7	dep	strength	772:779	arg1	the					760:762	the	760:762	the	760:762	Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively.					
34626727	4	8	dep	levels	657:662	arg1	%					668:668	0.2%	665:668	0.2%	665:668	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	4	8	dep	levels	657:662	arg1	%					674:674	0.4%	671:674	0.4%	671:674	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	4	8	dep	levels	657:662	arg1	%					683:683	0.6%	680:683	0.6%	680:683	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	3	9	theme	zeta	514:517	arg1	potential					519:527	zeta potential	514:527	zeta potential	514:527	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	0	10	theme	essential	106:114	arg1	oil					116:118	nanoencapsulated clove essential oil	83:118	nanoencapsulated clove essential oil	83:118	Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.					
34626727	3	11	contain	had	449:451	arg2	index					498:502	proper polydispersity index	476:502	proper polydispersity index (PDI)	476:508	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	11	contain	had	449:451	arg1	nanoparticles					435:447	the CEO-loaded zein-NaCas (C/ZN) nanoparticles	402:447	the CEO-loaded zein-NaCas (C/ZN) nanoparticles	402:447	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	11	contain	had	449:451	arg2	size					470:473	smaller particle size	453:473	smaller particle size	453:473	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	11	contain	had	449:451	arg2	PDI					505:507	PDI	505:507	PDI	505:507	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	11	contain	had	449:451	arg2	potential					519:527	zeta potential	514:527	zeta potential	514:527	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	11	contain	had	449:451	arg2	efficiency					561:570	higher encapsulation efficiency	540:570	higher encapsulation efficiency	540:570	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	2	12	theme	CEO	297:299	arg1	nanoparticles					309:321	clove essential oil (CEO)-loaded nanoparticles	276:321	clove essential oil (CEO)-loaded nanoparticles	276:321	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	9	13	theme	antibacterial	1464:1476	arg1	films					1478:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	8	14	with	film	1415:1418	arg1	nanoparticles					1430:1442	C/ZN nanoparticles	1425:1442	C/ZN nanoparticles	1425:1442	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	6	15	theme	C/ZN	981:984	arg1	nanoparticles					986:998	C/ZN nanoparticles	981:998	C/ZN nanoparticles	981:998	The infrared spectroscopy verified that the intermolecular hydrogen bonds exist between chitosan and C/ZN nanoparticles.					
34626727	8	16	dep	resistance	1306:1315	arg1	the					1296:1298	the	1296:1298	the	1296:1298	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	7	17	theme	improved	1132:1139	arg1	property					1155:1162	the improved antibacterial property	1128:1162	the improved antibacterial property	1128:1162	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	7	18	theme	controlled-release	1039:1056	arg1	property					1058:1065	a controlled-release property	1037:1065	a controlled-release property of CEO	1037:1072	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	2	19	theme	oil	292:294	arg1	nanoparticles					309:321	clove essential oil (CEO)-loaded nanoparticles	276:321	clove essential oil (CEO)-loaded nanoparticles	276:321	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	1	20	theme	physical	188:195	arg1	properties					212:221	physical and functional properties	188:221	physical and functional properties	188:221	The study aimed to obtain chitosan composite films with gratifying physical and functional properties.					
34626727	9	21	theme	great	1489:1493	arg1	potential					1495:1503	great potential	1489:1503	great potential	1489:1503	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	2	22	theme	1:2	328:330	arg1	zein					338:341	1:2 (w/w) zein	328:341	1:2 (w/w) zein	328:341	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	4	23	theme	chitosan	634:641	arg1	film					643:646	chitosan film	634:646	chitosan film at three levels (0.2%, 0.4% and 0.6%)	634:684	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	5	24	theme	break	785:789	arg1	elongation					791:800	break elongation	785:800	break elongation	785:800	Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively.					
34626727	1	25	theme	functional	201:210	arg1	properties					212:221	physical and functional properties	188:221	physical and functional properties	188:221	The study aimed to obtain chitosan composite films with gratifying physical and functional properties.					
34626727	7	26	theme	chitosan	1096:1103	arg1	films					1115:1119	the chitosan composite films	1092:1119	the chitosan composite films	1092:1119	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	6	27	theme	infrared	884:891	arg1	spectroscopy					893:904	The infrared spectroscopy	880:904	The infrared spectroscopy	880:904	The infrared spectroscopy verified that the intermolecular hydrogen bonds exist between chitosan and C/ZN nanoparticles.					
34626727	7	28	theme	chitosan	1005:1012	arg1	films					1024:1028	The chitosan composite films	1001:1028	The chitosan composite films	1001:1028	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	8	29	theme	light	1318:1322	arg1	blocking					1324:1331	light blocking	1318:1331	light blocking	1318:1331	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	3	30	theme	higher	540:545	arg1	efficiency					561:570	higher encapsulation efficiency	540:570	higher encapsulation efficiency	540:570	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	4	31	theme	acquired	583:590	arg1	nanoparticles					597:609	the acquired C/ZN nanoparticles	579:609	the acquired C/ZN nanoparticles	579:609	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	7	32	theme	composite	1105:1113	arg1	films					1115:1119	the chitosan composite films	1092:1119	the chitosan composite films	1092:1119	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	7	33	theme	composite	1014:1022	arg1	films					1024:1028	The chitosan composite films	1001:1028	The chitosan composite films	1001:1028	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	2	34	theme	-loaded	301:307	arg1	nanoparticles					309:321	clove essential oil (CEO)-loaded nanoparticles	276:321	clove essential oil (CEO)-loaded nanoparticles	276:321	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	3	35	theme	proper	476:481	arg1	PDI					505:507	PDI	505:507	PDI	505:507	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	35	theme	proper	476:481	arg1	index					498:502	proper polydispersity index	476:502	proper polydispersity index (PDI)	476:508	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	0	36	theme	packaging	46:54	arg1	films					56:60	chitosan-based packaging films	31:60	chitosan-based packaging films	31:60	Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.					
34626727	9	37	theme	current	1449:1455	arg1	films					1478:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	5	38	theme	tensile	764:770	arg1	strength					772:779	tensile strength	764:779	tensile strength	764:779	Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively.					
34626727	2	39	theme	Pickering	246:254	arg1	emulsion					256:263	a Pickering emulsion	244:263	a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas)	244:370	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	3	40	theme	zein-NaCas	417:426	arg1	nanoparticles					435:447	the CEO-loaded zein-NaCas (C/ZN) nanoparticles	402:447	the CEO-loaded zein-NaCas (C/ZN) nanoparticles	402:447	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	0	41	theme	chitosan-based	31:44	arg1	films					56:60	chitosan-based packaging films	31:60	chitosan-based packaging films	31:60	Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.					
34626727	8	42	theme	film	1415:1418	arg1	resistance					1306:1315	water resistance	1300:1315	water resistance	1300:1315	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	8	42	theme	film	1415:1418	arg1	blocking					1324:1331	light blocking	1318:1331	light blocking	1318:1331	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	8	42	theme	film	1415:1418	arg1	strength					1345:1352	mechanical strength	1334:1352	mechanical strength	1334:1352	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	8	42	theme	film	1415:1418	arg1	properties					1392:1401	antibacterial properties	1378:1401	antibacterial properties	1378:1401	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	3	43	theme	encapsulation	547:559	arg1	efficiency					561:570	higher encapsulation efficiency	540:570	higher encapsulation efficiency	540:570	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	4	44	theme	C/ZN	592:595	arg1	nanoparticles					597:609	the acquired C/ZN nanoparticles	579:609	the acquired C/ZN nanoparticles	579:609	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	5	45	theme	chitosan	805:812	arg1	films					814:818	chitosan films	805:818	chitosan films	805:818	Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively.					
34626727	8	46	theme	chitosan	1406:1413	arg1	film					1415:1418	chitosan film	1406:1418	chitosan film with C/ZN nanoparticles	1406:1442	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	8	47	theme	water	1300:1304	arg1	resistance					1306:1315	water resistance	1300:1315	water resistance	1300:1315	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	3	48	theme	smaller	453:459	arg1	size					470:473	smaller particle size	453:473	smaller particle size	453:473	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	0	49	theme	films	56:60	arg1	functionality					14:26	the functionality	10:26	the functionality of chitosan-based packaging films	10:60	Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.					
34626727	8	50	theme	C/ZN	1425:1428	arg1	nanoparticles					1430:1442	C/ZN nanoparticles	1425:1442	C/ZN nanoparticles	1425:1442	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	8	51	theme	mechanical	1334:1343	arg1	strength					1345:1352	mechanical strength	1334:1352	mechanical strength	1334:1352	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	3	52	theme	particle	461:468	arg1	size					470:473	smaller particle size	453:473	smaller particle size	453:473	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	9	53	contain	have	1484:1487	arg2	potential					1495:1503	great potential	1489:1503	great potential	1489:1503	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	9	53	contain	have	1484:1487	arg1	films					1478:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	4	54	theme	water	700:704	arg1	permeability					712:723	the water vapor permeability	696:723	the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa	696:751	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	2	55	theme	essential	282:290	arg1	nanoparticles					309:321	clove essential oil (CEO)-loaded nanoparticles	276:321	clove essential oil (CEO)-loaded nanoparticles	276:321	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	2	56	theme	w/w	333:335	arg1	zein					338:341	1:2 (w/w) zein	328:341	1:2 (w/w) zein	328:341	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	9	57	theme	food	1547:1550	arg1	system					1561:1566	food delivery system	1547:1566	food delivery system	1547:1566	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	9	58	theme	food	1525:1528	arg1	preservation					1530:1541	food preservation	1525:1541	food preservation	1525:1541	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	2	59	theme	clove	276:280	arg1	nanoparticles					309:321	clove essential oil (CEO)-loaded nanoparticles	276:321	clove essential oil (CEO)-loaded nanoparticles	276:321	First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas).					
34626727	3	60	theme	polydispersity	483:496	arg1	PDI					505:507	PDI	505:507	PDI	505:507	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	60	theme	polydispersity	483:496	arg1	index					498:502	proper polydispersity index	476:502	proper polydispersity index (PDI)	476:508	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	9	61	theme	edible	1457:1462	arg1	films					1478:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films	1445:1482	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	7	62	theme	larger	1176:1181	arg1	zones					1194:1198	larger inhibition zones	1176:1198	larger inhibition zones	1176:1198	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	5	63	theme	films	814:818	arg1	strength					772:779	tensile strength	764:779	tensile strength	764:779	Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively.					
34626727	5	63	theme	films	814:818	arg1	elongation					791:800	break elongation	785:800	break elongation	785:800	Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively.					
34626727	8	64	theme	antibacterial	1378:1390	arg1	properties					1392:1401	antibacterial properties	1378:1401	antibacterial properties	1378:1401	In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles.					
34626727	1	65	theme	chitosan	147:154	arg1	films					166:170	chitosan composite films	147:170	chitosan composite films	147:170	The study aimed to obtain chitosan composite films with gratifying physical and functional properties.					
34626727	3	66	theme	CEO-loaded	406:415	arg1	C/ZN					429:432	C/ZN	429:432	C/ZN	429:432	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	3	66	theme	CEO-loaded	406:415	arg1	zein-NaCas					417:426	CEO-loaded zein-NaCas	406:426	the CEO-loaded zein-NaCas (C/ZN) nanoparticles	402:447	We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency.					
34626727	9	67	theme	delivery	1552:1559	arg1	system					1561:1566	food delivery system	1547:1566	food delivery system	1547:1566	The current edible antibacterial films have great potential on applications for food preservation and food delivery system.					
34626727	7	68	theme	inhibition	1183:1192	arg1	zones					1194:1198	larger inhibition zones	1176:1198	larger inhibition zones	1176:1198	The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm).					
34626727	4	69	theme	vapor	706:710	arg1	permeability					712:723	the water vapor permeability	696:723	the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa	696:751	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	1	70	theme	composite	156:164	arg1	films					166:170	chitosan composite films	147:170	chitosan composite films	147:170	The study aimed to obtain chitosan composite films with gratifying physical and functional properties.					
34626727	4	71	from	levels	657:662	arg1	film					643:646	chitosan film	634:646	chitosan film at three levels (0.2%, 0.4% and 0.6%)	634:684	Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10-6 g·s-1·m-1·Pa.					
34626727	6	72	theme	hydrogen	939:946	arg1	bonds					948:952	the intermolecular hydrogen bonds	920:952	the intermolecular hydrogen bonds	920:952	The infrared spectroscopy verified that the intermolecular hydrogen bonds exist between chitosan and C/ZN nanoparticles.					
33080108	21	0	dep	CONCLUSION	2833:2842	arg1	increase					2931:2938	increase	2931:2938	increase the ALP activity and calcium content	2931:2975	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	21	0	dep	CONCLUSION	2833:2842	arg1	enhance					2899:2905	enhance	2899:2905	enhance osteogenic efficacy	2899:2925	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	4	1	theme	chitosan	593:600	arg1	nanospheres					602:612	chitosan nanospheres	593:612	chitosan nanospheres	593:612	In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity.					
33080108	20	2	theme	microspheres/CHA	2705:2720	arg1	group					2732:2736	the rhBMP-2 microspheres/CHA composite group	2693:2736	the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg)	2693:2779	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	2	3	theme	new	387:389	arg1	methods					391:397	new methods	387:397	new methods	387:397	Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed.					
33080108	15	4	located	observed	1962:1969	arg2	surface					1934:1940	a smooth and unwrinkled surface	1910:1940	a smooth and unwrinkled surface of the spheres	1910:1955	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	15	4	located	observed	1962:1969	arg1	microspheres					2000:2011	all chitosan (CS)/rhBMP-2 microspheres	1974:2011	all chitosan (CS)/rhBMP-2 microspheres	1974:2011	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	15	4	located	observed	1962:1969	arg2	regularity					1895:1904	Spherical regularity	1885:1904	Spherical regularity	1885:1904	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	9	5	theme	0.5	1387:1389	arg1	mg					1391:1392	mg	1391:1392	mg	1391:1392	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	6	6	dep	-loaded	894:900	arg1	CHA					842:844	the CHA	838:844	the CHA composite with recombinant human BMP-2	838:883	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	10	7	theme	scanning	1517:1524	arg1	microscope					1535:1544	a scanning electron microscope	1515:1544	a scanning electron microscope	1515:1544	The microsphere morphology was scanned and analyzed using a scanning electron microscope.					
33080108	21	8	theme	calcium	2961:2967	arg1	content					2969:2975	calcium content	2961:2975	calcium content	2961:2975	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	18	9	located	found	2316:2320	arg2	osteogenesis					2299:2310	No ectopic osteogenesis	2288:2310	No ectopic osteogenesis	2288:2310	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	18	9	located	found	2316:2320	arg1	canal					2339:2343	the vertebral canal	2325:2343	the vertebral canal	2325:2343	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	18	9	located	found	2316:2320	arg1	tissues					2361:2367	other muscle tissues	2348:2367	other muscle tissues	2348:2367	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	6	10	theme	human	873:877	arg1	BMP-2					879:883	recombinant human BMP-2	861:883	recombinant human BMP-2	861:883	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	15	11	theme	Spherical	1885:1893	arg1	regularity					1895:1904	Spherical regularity	1885:1904	Spherical regularity	1885:1904	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	2	12	theme	spinal	306:311	arg1	fusion					313:318	spinal fusion	306:318	spinal fusion	306:318	Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed.					
33080108	19	13	theme	composite	2438:2446	arg1	group					2448:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	11	14	dep	tomography	1562:1571	arg1	examination					1573:1583	examination	1573:1583	examination	1573:1583	Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery.					
33080108	11	14	dep	tomography	1562:1571	arg1	reconstruction					1607:1620	three-dimensional reconstruction	1589:1620	three-dimensional reconstruction	1589:1620	Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery.					
33080108	15	15	theme	unwrinkled	1923:1932	arg1	surface					1934:1940	a smooth and unwrinkled surface	1910:1940	a smooth and unwrinkled surface of the spheres	1910:1955	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	3	16	from	osteogenesis	510:521	arg1	fusion					533:538	spinal fusion	526:538	spinal fusion	526:538	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	19	17	theme	implantation	2387:2398	arg1	4 weeks					2376:2382	4 weeks	2376:2382	4 weeks of implantation	2376:2398	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	1	18	theme	surgical	207:214	arg1	interventions					216:228	the most common surgical interventions	191:228	the most common surgical interventions for spine reconstruction	191:253	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	6	19	dep	no	787:788	arg1	to					780:781	to	780:781	to	780:781	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	15	20	theme	smooth	1912:1917	arg1	surface					1934:1940	a smooth and unwrinkled surface	1910:1940	a smooth and unwrinkled surface of the spheres	1910:1955	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	5	21	from	implants	743:750	arg1	fusion					760:765	bone fusion	755:765	bone fusion	755:765	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	17	22	theme	rhBMP-2/CHA	2259:2269	arg1	group					2281:2285	the rhBMP-2/CHA composite group	2255:2285	the rhBMP-2/CHA composite group	2255:2285	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	2	23	dep	efforts	268:274	arg1	promote					279:285	promote	279:285	to promote osteogenesis after spinal fusion	276:318	Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed.					
33080108	14	24	dep	RESULTS	1803:1809	arg1	composite					1848:1856	The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1811:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1803:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite was successfully prepared.					
33080108	7	25	theme	spinal	1047:1052	arg1	fusion					1054:1059	spinal fusion	1047:1059	spinal fusion	1047:1059	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	20	26	theme	calcium	2674:2680	arg1	content					2682:2688	calcium content	2674:2688	calcium content	2674:2688	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	13	27	theme	calcium	1772:1778	arg1	content					1780:1786	calcium content	1772:1786	calcium content	1772:1786	Both alkaline phosphatase (ALP) and calcium content were measured.					
33080108	8	28	dep	METHODS	1062:1068	arg1	microspheres					1089:1100	The rhBMP-2-loaded microspheres	1070:1100	The rhBMP-2-loaded microspheres	1070:1100	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	7	29	theme	CHA	975:977	arg1	implant					979:985	the CHA implant	971:985	the CHA implant with rhBMP-2-loaded chitosan nanospheres	971:1026	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	9	30	theme	negative	1433:1440	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	30	theme	negative	1433:1440	arg1	group					1450:1454	the negative control group	1429:1454	the negative control group	1429:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	6	31	theme	chitosan	902:909	arg1	nanospheres					911:921	the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres	838:921	the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres	838:921	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	18	32	theme	other	2348:2352	arg1	tissues					2361:2367	other muscle tissues	2348:2367	other muscle tissues	2348:2367	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	16	33	located	found	2070:2074	arg2	behaviors					2055:2063	abnormal behaviors	2046:2063	abnormal behaviors	2046:2063	No side effects, infections, or abnormal behaviors were found in the animals.					
33080108	16	33	located	found	2070:2074	arg2	infections					2031:2040	infections	2031:2040	infections	2031:2040	No side effects, infections, or abnormal behaviors were found in the animals.					
33080108	16	33	located	found	2070:2074	arg2	effects					2022:2028	No side effects	2014:2028	No side effects	2014:2028	No side effects, infections, or abnormal behaviors were found in the animals.					
33080108	16	33	located	found	2070:2074	arg1	animals					2083:2089	the animals	2079:2089	the animals	2079:2089	No side effects, infections, or abnormal behaviors were found in the animals.					
33080108	21	34	theme	CS	2878:2879	arg1	nanospheres					2881:2891	rhBMP-2-loaded CS nanospheres	2863:2891	rhBMP-2-loaded CS nanospheres	2863:2891	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	21	35	with	composite	2848:2856	arg1	nanospheres					2881:2891	rhBMP-2-loaded CS nanospheres	2863:2891	rhBMP-2-loaded CS nanospheres	2863:2891	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	19	36	theme	bone	2527:2530	arg1	cells					2532:2536	bone cells	2527:2536	bone cells	2527:2536	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	1	37	theme	Spinal	167:172	arg1	fusion					174:179	OBJECTIVE Spinal fusion	157:179	OBJECTIVE Spinal fusion	157:179	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	1	38	theme	OBJECTIVE	157:165	arg1	fusion					174:179	OBJECTIVE Spinal fusion	157:179	OBJECTIVE Spinal fusion	157:179	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	22	39	theme	new	3065:3067	arg1	insight					3069:3075	new insight	3065:3075	new insight into the role of BMP-2 in osteogenesis	3065:3114	These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.					
33080108	17	40	from	implant	2190:2196	arg1	group					2281:2285	the rhBMP-2/CHA composite group	2255:2285	the rhBMP-2/CHA composite group	2255:2285	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	17	40	from	implant	2190:2196	arg1	group					2245:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	4	41	from	effects	632:638	arg1	activity					663:670	osteogenic activity	652:670	osteogenic activity	652:670	In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity.					
33080108	9	42	theme	rhBMP-2	1252:1258	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	42	theme	rhBMP-2	1252:1258	arg1	group					1287:1291	the rhBMP-2 microspheres/CHA composite group	1248:1291	the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2)	1248:1319	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	14	43	theme	rhBMP-2/CHA	1835:1845	arg1	composite					1848:1856	The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1811:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1803:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite was successfully prepared.					
33080108	12	44	theme	eosin	1680:1684	arg1	staining					1686:1693	Hematoxylin and eosin staining	1664:1693	Hematoxylin and eosin staining	1664:1693	Hematoxylin and eosin staining was conducted for histological analysis.					
33080108	22	45	theme	spinal	3041:3046	arg1	fusion					3048:3053	spinal fusion	3041:3053	spinal fusion	3041:3053	These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.					
33080108	0	46	theme	Protein-2-Loaded	90:105	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	9	47	theme	composite	1277:1285	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	47	theme	composite	1277:1285	arg1	group					1287:1291	the rhBMP-2 microspheres/CHA composite group	1248:1291	the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2)	1248:1319	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	14	48	theme	CHA	1830:1832	arg1	composite					1848:1856	The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1811:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1803:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite was successfully prepared.					
33080108	7	49	with	implant	979:985	arg1	nanospheres					1016:1026	rhBMP-2-loaded chitosan nanospheres	992:1026	rhBMP-2-loaded chitosan nanospheres	992:1026	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	17	50	theme	rhBMP-2	2210:2216	arg1	group					2245:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	17	51	theme	bone	2149:2152	arg1	fusion					2154:2159	bone fusion	2149:2159	bone fusion	2149:2159	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	5	52	theme	used	738:741	arg1	implants					743:750	the most frequently used implants	718:750	the most frequently used implants in bone fusion	718:765	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	19	53	theme	bone	2610:2613	arg1	formation					2622:2630	obvious bone tissue formation	2602:2630	obvious bone tissue formation	2602:2630	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	17	54	theme	composite	2235:2243	arg1	group					2245:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	20	55	from	activity	2661:2668	arg1	group					2732:2736	the rhBMP-2 microspheres/CHA composite group	2693:2736	the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg)	2693:2779	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	4	56	theme	nanospheres	602:612	arg1	delivery					581:588	the delivery	577:588	the delivery of chitosan nanospheres	577:612	In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity.					
33080108	9	57	theme	0.5	1305:1307	arg1	mg					1309:1310	mg	1309:1310	mg	1309:1310	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	12	58	theme	Hematoxylin	1664:1674	arg1	staining					1686:1693	Hematoxylin and eosin staining	1664:1693	Hematoxylin and eosin staining	1664:1693	Hematoxylin and eosin staining was conducted for histological analysis.					
33080108	22	59	theme	BMP-2	3094:3098	arg1	role					3086:3089	the role	3082:3089	the role of BMP-2 in osteogenesis	3082:3114	These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.					
33080108	0	60	theme	Morphogenetic	76:88	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	20	61	theme	ALP	2657:2659	arg1	activity					2661:2668	ALP activity	2657:2668	ALP activity	2657:2668	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	14	62	theme	rhBMP-2-loaded	1815:1828	arg1	composite					1848:1856	The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1811:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite	1803:1856	RESULTS The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite was successfully prepared.					
33080108	0	63	theme	Coralline	15:23	arg1	Implant					40:46	Coralline Hydroxyapatite Implant	15:46	Coralline Hydroxyapatite Implant	15:46	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	11	64	dep	4 weeks	1637:1643	arg1	surgery					1655:1661	the surgery	1651:1661	the surgery	1651:1661	Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery.					
33080108	6	65	theme	composite	846:854	arg1	CHA					842:844	the CHA	838:844	the CHA composite with recombinant human BMP-2	838:883	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	18	66	theme	ectopic	2291:2297	arg1	osteogenesis					2299:2310	No ectopic osteogenesis	2288:2310	No ectopic osteogenesis	2288:2310	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	15	67	theme	/rhBMP-2	1991:1998	arg1	microspheres					2000:2011	all chitosan (CS)/rhBMP-2 microspheres	1974:2011	all chitosan (CS)/rhBMP-2 microspheres	1974:2011	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	21	68	theme	ALP	2944:2946	arg1	activity					2948:2955	the ALP activity	2940:2955	the ALP activity	2940:2955	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	3	69	theme	reported	470:477	arg1	factor					479:484	a widely reported factor	461:484	a widely reported factor that can facilitate the osteogenesis in spinal fusion	461:538	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	3	69	theme	reported	470:477	arg1	protein-2					440:448	The bone morphogenetic protein-2	417:448	The bone morphogenetic protein-2 (BMP-2)	417:456	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	0	70	theme	Implant	40:46	arg1	Preparation					0:10	Preparation	0:10	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.	0:155	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	9	71	theme	rhBMP-2-loaded	1326:1339	arg1	group					1369:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group	1322:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2)	1322:1401	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	71	theme	rhBMP-2-loaded	1326:1339	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	8	72	theme	rhBMP-2-loaded	1074:1087	arg1	microspheres					1089:1100	The rhBMP-2-loaded microspheres	1070:1100	The rhBMP-2-loaded microspheres	1070:1100	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	3	73	theme	morphogenetic	426:438	arg1	factor					479:484	a widely reported factor	461:484	a widely reported factor that can facilitate the osteogenesis in spinal fusion	461:538	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	3	73	theme	morphogenetic	426:438	arg1	protein-2					440:448	The bone morphogenetic protein-2	417:448	The bone morphogenetic protein-2 (BMP-2)	417:456	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	3	73	theme	morphogenetic	426:438	arg1	BMP-2					451:455	BMP-2	451:455	BMP-2	451:455	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	4	74	theme	previous	544:551	arg1	research					553:560	previous research	544:560	previous research	544:560	In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity.					
33080108	0	75	theme	Bone	71:74	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	0	76	theme	Recombinant	53:63	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	19	77	theme	microspheres/CHA	2421:2436	arg1	group					2448:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	15	78	theme	chitosan	1978:1985	arg1	microspheres					2000:2011	all chitosan (CS)/rhBMP-2 microspheres	1974:2011	all chitosan (CS)/rhBMP-2 microspheres	1974:2011	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	17	79	theme	new	2126:2128	arg1	formation					2135:2143	obvious new bone formation	2118:2143	obvious new bone formation	2118:2143	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	0	80	with	Preparation	0:10	arg1	Efficacy					147:154	Its Osteogenic Efficacy	132:154	Its Osteogenic Efficacy	132:154	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	0	80	with	Preparation	0:10	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	2	81	theme	clinical	364:371	arg1	challenge					373:381	a clinical challenge	362:381	a clinical challenge	362:381	Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed.					
33080108	19	82	theme	composite	2474:2482	arg1	group					2484:2488	the rhBMP-2/CHA composite group	2458:2488	the rhBMP-2/CHA composite group	2458:2488	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	8	83	theme	rhBMP-2	1121:1127	arg1	composite					1110:1118	CHA composite	1106:1118	CHA composite (rhBMP-2 microspheres/CHA)	1106:1145	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	8	83	theme	rhBMP-2	1121:1127	arg1	microspheres/CHA					1129:1144	rhBMP-2 microspheres/CHA	1121:1144	rhBMP-2 microspheres/CHA	1121:1144	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	6	84	theme	-loaded	894:900	arg1	nanospheres					911:921	the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres	838:921	the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres	838:921	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	7	85	from	osteogenesis	1031:1042	arg1	fusion					1054:1059	spinal fusion	1047:1059	spinal fusion	1047:1059	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	20	86	theme	composite	2722:2730	arg1	group					2732:2736	the rhBMP-2 microspheres/CHA composite group	2693:2736	the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg)	2693:2779	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	3	87	theme	spinal	526:531	arg1	fusion					533:538	spinal fusion	526:538	spinal fusion	526:538	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	19	88	theme	trabecular	2556:2565	arg1	bone					2567:2570	trabecular bone	2556:2570	trabecular bone	2556:2570	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	10	89	theme	electron	1526:1533	arg1	microscope					1535:1544	a scanning electron microscope	1515:1544	a scanning electron microscope	1515:1544	The microsphere morphology was scanned and analyzed using a scanning electron microscope.					
33080108	5	90	theme	coralline	677:685	arg1	implants					743:750	the most frequently used implants	718:750	the most frequently used implants in bone fusion	718:765	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	5	90	theme	coralline	677:685	arg1	CHA					703:705	CHA	703:705	CHA	703:705	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	5	90	theme	coralline	677:685	arg1	one					711:713	one	711:713	one	711:713	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	5	90	theme	coralline	677:685	arg1	hydroxyapatite					687:700	The coralline hydroxyapatite	673:700	The coralline hydroxyapatite (CHA)	673:706	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	9	91	theme	mg	1391:1392	arg1	rhBMP-2					1394:1400	0.5 mg rhBMP-2	1387:1400	0.5 mg rhBMP-2	1387:1400	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	20	92	theme	other	2819:2823	arg1	groups					2825:2830	all other groups	2815:2830	all other groups	2815:2830	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	9	93	dep	groups	1240:1245	arg1	group					1369:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group	1322:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2)	1322:1401	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	93	dep	groups	1240:1245	arg1	group					1418:1422	the blank CHA group	1404:1422	the blank CHA group	1404:1422	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	93	dep	groups	1240:1245	arg1	group					1287:1291	the rhBMP-2 microspheres/CHA composite group	1248:1291	the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2)	1248:1319	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	93	dep	groups	1240:1245	arg1	group					1450:1454	the negative control group	1429:1454	the negative control group	1429:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	93	dep	groups	1240:1245	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	6	94	theme	recombinant	861:871	arg1	BMP-2					879:883	recombinant human BMP-2	861:883	recombinant human BMP-2	861:883	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	8	95	used	used	1170:1173	arg2	METHODS					1062:1068	METHODS	1062:1068	METHODS	1062:1068	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	18	96	theme	muscle	2354:2359	arg1	tissues					2361:2367	other muscle tissues	2348:2367	other muscle tissues	2348:2367	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	7	97	from	effects	960:966	arg1	osteogenesis					1031:1042	osteogenesis	1031:1042	osteogenesis in spinal fusion	1031:1059	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	20	98	theme	rhBMP-2	2697:2703	arg1	group					2732:2736	the rhBMP-2 microspheres/CHA composite group	2693:2736	the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg)	2693:2779	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	1	99	theme	common	200:205	arg1	interventions					216:228	the most common surgical interventions	191:228	the most common surgical interventions for spine reconstruction	191:253	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	9	100	theme	SD	1209:1210	arg1	rats					1212:1215	All SD rats	1205:1215	All SD rats	1205:1215	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	2	101	theme	spinal	340:345	arg1	fusion					347:352	spinal fusion	340:352	spinal fusion	340:352	Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed.					
33080108	1	102	theme	interventions	216:228	arg1	one					184:186	one	184:186	one	184:186	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	1	102	theme	interventions	216:228	arg1	interventions					216:228	the most common surgical interventions	191:228	the most common surgical interventions for spine reconstruction	191:253	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	22	103	from	role	3086:3089	arg1	osteogenesis					3103:3114	osteogenesis	3103:3114	osteogenesis	3103:3114	These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.					
33080108	4	104	theme	BMP-2	643:647	arg1	effects					632:638	the effects	628:638	the effects of BMP-2 on osteogenic activity	628:670	In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity.					
33080108	9	105	theme	blank	1408:1412	arg1	group					1418:1422	the blank CHA group	1404:1422	the blank CHA group	1404:1422	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	105	theme	blank	1408:1412	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	1	106	theme	spine	234:238	arg1	reconstruction					240:253	spine reconstruction	234:253	spine reconstruction	234:253	OBJECTIVE Spinal fusion is one of the most common surgical interventions for spine reconstruction.					
33080108	8	107	theme	rats	1199:1202	arg1	implantation					1179:1190	implantation	1179:1190	implantation of the rats	1179:1202	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	17	108	theme	composite	2271:2279	arg1	group					2281:2285	the rhBMP-2/CHA composite group	2255:2285	the rhBMP-2/CHA composite group	2255:2285	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	4	109	theme	osteogenic	652:661	arg1	activity					663:670	osteogenic activity	652:670	osteogenic activity	652:670	In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity.					
33080108	13	110	theme	alkaline	1741:1748	arg1	ALP					1763:1765	ALP	1763:1765	ALP	1763:1765	Both alkaline phosphatase (ALP) and calcium content were measured.					
33080108	13	110	theme	alkaline	1741:1748	arg1	phosphatase					1750:1760	alkaline phosphatase	1741:1760	alkaline phosphatase (ALP)	1741:1766	Both alkaline phosphatase (ALP) and calcium content were measured.					
33080108	7	111	theme	rhBMP-2-loaded	992:1005	arg1	nanospheres					1016:1026	rhBMP-2-loaded chitosan nanospheres	992:1026	rhBMP-2-loaded chitosan nanospheres	992:1026	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	6	112	theme	nanospheres	911:921	arg1	efficacy					826:833	the osteogenic efficacy	811:833	the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres	811:921	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	19	113	theme	osteoid	2491:2497	arg1	tissues					2499:2505	osteoid tissues	2491:2505	osteoid tissues	2491:2505	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	9	114	contain	containing	1376:1385	arg1	group					1369:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group	1322:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2)	1322:1401	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	114	contain	containing	1376:1385	arg2	rhBMP-2					1394:1400	0.5 mg rhBMP-2	1387:1400	0.5 mg rhBMP-2	1387:1400	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	114	contain	containing	1376:1385	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	7	115	theme	chitosan	1007:1014	arg1	nanospheres					1016:1026	rhBMP-2-loaded chitosan nanospheres	992:1026	rhBMP-2-loaded chitosan nanospheres	992:1026	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	19	116	theme	bone	2539:2542	arg1	marrow					2544:2549	bone marrow	2539:2549	bone marrow	2539:2549	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	7	117	theme	implant	979:985	arg1	effects					960:966	the effects	956:966	the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion	956:1059	This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion.					
33080108	9	118	theme	control	1442:1448	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	118	theme	control	1442:1448	arg1	group					1450:1454	the negative control group	1429:1454	the negative control group	1429:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	5	119	theme	bone	755:758	arg1	fusion					760:765	bone fusion	755:765	bone fusion	755:765	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	9	120	theme	CHA	1414:1416	arg1	group					1418:1422	the blank CHA group	1404:1422	the blank CHA group	1404:1422	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	120	theme	CHA	1414:1416	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	12	121	theme	histological	1713:1724	arg1	analysis					1726:1733	histological analysis	1713:1733	histological analysis	1713:1733	Hematoxylin and eosin staining was conducted for histological analysis.					
33080108	18	122	theme	vertebral	2329:2337	arg1	canal					2339:2343	the vertebral canal	2325:2343	the vertebral canal	2325:2343	No ectopic osteogenesis was found in the vertebral canal or other muscle tissues.					
33080108	0	123	theme	Chitosan	107:114	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	9	124	theme	microspheres/CHA	1260:1275	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	124	theme	microspheres/CHA	1260:1275	arg1	group					1287:1291	the rhBMP-2 microspheres/CHA composite group	1248:1291	the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2)	1248:1319	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	22	125	from	method	3014:3019	arg1	fusion					3048:3053	spinal fusion	3041:3053	spinal fusion	3041:3053	These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.					
33080108	20	126	from	content	2682:2688	arg1	group					2732:2736	the rhBMP-2 microspheres/CHA composite group	2693:2736	the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg)	2693:2779	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	19	127	theme	obvious	2602:2608	arg1	formation					2622:2630	obvious bone tissue formation	2602:2630	obvious bone tissue formation	2602:2630	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	17	128	theme	microspheres/CHA	2218:2233	arg1	group					2245:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	the rhBMP-2 microspheres/CHA composite group	2206:2249	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	21	129	theme	rhBMP-2-loaded	2863:2876	arg1	nanospheres					2881:2891	rhBMP-2-loaded CS nanospheres	2863:2891	rhBMP-2-loaded CS nanospheres	2863:2891	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	0	130	theme	Osteogenic	136:145	arg1	Efficacy					147:154	Its Osteogenic Efficacy	132:154	Its Osteogenic Efficacy	132:154	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	19	131	theme	mature	2584:2589	arg1	sclerotin					2591:2599	mature sclerotin	2584:2599	mature sclerotin	2584:2599	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	22	132	theme	novel	3008:3012	arg1	method					3014:3019	a novel method	3006:3019	a novel method for osteogenesis in spinal fusion	3006:3053	These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.					
33080108	15	133	theme	spheres	1949:1955	arg1	regularity					1895:1904	Spherical regularity	1885:1904	Spherical regularity	1885:1904	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	15	133	theme	spheres	1949:1955	arg1	surface					1934:1940	a smooth and unwrinkled surface	1910:1940	a smooth and unwrinkled surface of the spheres	1910:1955	Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres.					
33080108	5	134	theme	implants	743:750	arg1	implants					743:750	the most frequently used implants	718:750	the most frequently used implants in bone fusion	718:765	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	5	134	theme	implants	743:750	arg1	one					711:713	one	711:713	one	711:713	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	5	134	theme	implants	743:750	arg1	hydroxyapatite					687:700	The coralline hydroxyapatite	673:700	The coralline hydroxyapatite (CHA)	673:706	The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion.					
33080108	9	135	theme	mg	1309:1310	arg1	rhBMP-2					1312:1318	0.5 mg rhBMP-2	1305:1318	0.5 mg rhBMP-2	1305:1318	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	6	136	with	composite	846:854	arg1	BMP-2					879:883	recombinant human BMP-2	861:883	recombinant human BMP-2	861:883	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	3	137	theme	bone	421:424	arg1	factor					479:484	a widely reported factor	461:484	a widely reported factor that can facilitate the osteogenesis in spinal fusion	461:538	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	3	137	theme	bone	421:424	arg1	protein-2					440:448	The bone morphogenetic protein-2	417:448	The bone morphogenetic protein-2 (BMP-2)	417:456	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	3	137	theme	bone	421:424	arg1	BMP-2					451:455	BMP-2	451:455	BMP-2	451:455	The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion.					
33080108	19	138	located	found	2516:2520	arg1	group					2484:2488	the rhBMP-2/CHA composite group	2458:2488	the rhBMP-2/CHA composite group	2458:2488	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	19	138	located	found	2516:2520	arg1	group					2448:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	19	138	located	found	2516:2520	arg2	tissues					2499:2505	osteoid tissues	2491:2505	osteoid tissues	2491:2505	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	0	139	theme	Hydroxyapatite	25:38	arg1	Implant					40:46	Coralline Hydroxyapatite Implant	15:46	Coralline Hydroxyapatite Implant	15:46	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	19	140	theme	rhBMP-2	2413:2419	arg1	group					2448:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	the rhBMP-2 microspheres/CHA composite group	2409:2452	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	19	141	theme	tissue	2615:2620	arg1	formation					2622:2630	obvious bone tissue formation	2602:2630	obvious bone tissue formation	2602:2630	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
33080108	16	142	theme	side	2017:2020	arg1	effects					2022:2028	No side effects	2014:2028	No side effects	2014:2028	No side effects, infections, or abnormal behaviors were found in the animals.					
33080108	9	143	contain	containing	1294:1303	arg2	rhBMP-2					1312:1318	0.5 mg rhBMP-2	1305:1318	0.5 mg rhBMP-2	1305:1318	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	143	contain	containing	1294:1303	arg1	group					1287:1291	the rhBMP-2 microspheres/CHA composite group	1248:1291	the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2)	1248:1319	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	143	contain	containing	1294:1303	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	20	144	dep	group	2732:2736	arg1	kat/g					2751:2755	6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg	2739:2778	kat/g	2751:2755	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	20	144	dep	group	2732:2736	arg1	μg/mg					2774:2778	6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg	2739:2778	μg/mg	2774:2778	Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups.					
33080108	17	145	theme	surgery	2109:2115	arg1	4 weeks					2098:2104	4 weeks	2098:2104	4 weeks of surgery	2098:2115	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	11	146	theme	Micro-computed	1547:1560	arg1	tomography					1562:1571	Micro-computed tomography examination and three-dimensional reconstruction	1547:1620	Micro-computed tomography examination and three-dimensional reconstruction	1547:1620	Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery.					
33080108	0	147	theme	Human	65:69	arg1	Nanospheres					116:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres	53:126	Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein-2-Loaded Chitosan Nanospheres and Its Osteogenic Efficacy.					
33080108	9	148	theme	CHA	1341:1343	arg1	group					1369:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group	1322:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2)	1322:1401	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	148	theme	CHA	1341:1343	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	8	149	theme	CHA	1106:1108	arg1	composite					1110:1118	CHA composite	1106:1118	CHA composite (rhBMP-2 microspheres/CHA)	1106:1145	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	8	149	theme	CHA	1106:1108	arg1	microspheres/CHA					1129:1144	rhBMP-2 microspheres/CHA	1121:1144	rhBMP-2 microspheres/CHA	1121:1144	METHODS The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats.					
33080108	10	150	theme	microsphere	1461:1471	arg1	morphology					1473:1482	The microsphere morphology	1457:1482	The microsphere morphology	1457:1482	The microsphere morphology was scanned and analyzed using a scanning electron microscope.					
33080108	21	151	theme	osteogenic	2907:2916	arg1	efficacy					2918:2925	osteogenic efficacy	2907:2925	osteogenic efficacy	2907:2925	CONCLUSION The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content.					
33080108	17	152	theme	obvious	2118:2124	arg1	formation					2135:2143	obvious new bone formation	2118:2143	obvious new bone formation	2118:2143	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	9	153	theme	rhBMP-2/CHA	1346:1356	arg1	group					1369:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group	1322:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2)	1322:1401	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	153	theme	rhBMP-2/CHA	1346:1356	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	6	154	theme	osteogenic	815:824	arg1	efficacy					826:833	the osteogenic efficacy	811:833	the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres	811:921	However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres.					
33080108	16	155	theme	abnormal	2046:2053	arg1	behaviors					2055:2063	abnormal behaviors	2046:2063	abnormal behaviors	2046:2063	No side effects, infections, or abnormal behaviors were found in the animals.					
33080108	17	156	theme	bone	2130:2133	arg1	formation					2135:2143	obvious new bone formation	2118:2143	obvious new bone formation	2118:2143	After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group.					
33080108	11	157	theme	three-dimensional	1589:1605	arg1	reconstruction					1607:1620	three-dimensional reconstruction	1589:1620	three-dimensional reconstruction	1589:1620	Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery.					
33080108	9	158	theme	composite	1359:1367	arg1	group					1369:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group	1322:1373	the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2)	1322:1401	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	9	158	theme	composite	1359:1367	arg1	groups					1240:1245	four groups	1235:1245	four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group	1235:1454	All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group.					
33080108	19	159	theme	rhBMP-2/CHA	2462:2472	arg1	group					2484:2488	the rhBMP-2/CHA composite group	2458:2488	the rhBMP-2/CHA composite group	2458:2488	After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen.					
34726686	0	0	theme	damaged	158:164	arg1	tissue					176:181	damaged cartilage tissue	158:181	damaged cartilage tissue	158:181	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	8	1	theme	full	1762:1765	arg1	closure					1767:1773	a full closure	1760:1773	a full closure of lesions on the cartilage tissue	1760:1808	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	1	2	theme	articular	273:281	arg1	lesions					293:299	articular cartilage lesions	273:299	articular cartilage lesions	273:299	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	3	3	from	located	851:857	arg1	shell					866:870	the shell	862:870	the shell	862:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	4	4	theme	two-stage	1001:1009	arg1	release					1026:1032	a two-stage rapid and slow release	999:1032	a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site	999:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	3	5	theme	lactic-co-glycolic	724:741	arg1	poly					719:722	porous poly	712:722	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell	712:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	5	theme	lactic-co-glycolic	724:741	arg1	acid					743:746	lactic-co-glycolic acid	724:746	lactic-co-glycolic acid	724:746	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	1	6	theme	Injectable	184:193	arg1	hydrogels					206:214	Injectable cell-based hydrogels	184:214	Injectable cell-based hydrogels	184:214	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	3	7	theme	magnetic	756:763	arg1	PPMMs					780:784	PPMMs	780:784	PPMMs	780:784	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	7	theme	magnetic	756:763	arg1	microcapsules					765:777	magnetic microcapsules	756:777	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell	712:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	8	8	theme	cartilage	1793:1801	arg1	tissue					1803:1808	the cartilage tissue	1789:1808	the cartilage tissue	1789:1808	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	7	9	theme	deep	1563:1566	arg1	tissue					1573:1578	the deep zone tissue	1559:1578	the deep zone tissue	1559:1578	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	5	10	from	receptors	1208:1216	arg1	chains					1236:1241	the HA polymer chains	1221:1241	the HA polymer chains of LbL-PPMMs toward the surface of the damaged site	1221:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	4	11	theme	GSH	1037:1039	arg1	release					1026:1032	a two-stage rapid and slow release	999:1032	a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site	999:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	0	12	theme	chondrocyte	120:130	arg1	array					132:136	in situ chondrocyte array	112:136	in situ chondrocyte array	112:136	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	1	13	theme	interest	399:406	arg1	interest					399:406	interest	399:406	interest	399:406	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	1	13	theme	interest	399:406	arg1	site					391:394	the site	387:394	the site of interest to repair the cartilage tissue	387:437	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	6	14	theme	smooth	1444:1449	arg1	surface					1451:1457	a more even and smooth surface	1428:1457	a more even and smooth surface than other groups	1428:1475	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	1	15	theme	surgical	222:229	arg1	operation					231:239	surgical operation	222:239	surgical operation	222:239	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	4	16	theme	layer-by-layer	913:926	arg1	assembly					934:941	layer-by-layer (LbL) assembly	913:941	layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs)	913:985	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	9	17	theme	promising	1862:1870	arg1	potential					1872:1880	the highly promising potential	1851:1880	the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair	1851:1948	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	1	18	theme	cartilage	422:430	arg1	tissue					432:437	the cartilage tissue	418:437	the cartilage tissue	418:437	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	7	19	theme	cartilage	1648:1656	arg1	tissue					1658:1663	normal cartilage tissue	1641:1663	normal cartilage tissue	1641:1663	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	0	20	theme	cartilage	166:174	arg1	tissue					176:181	damaged cartilage tissue	158:181	damaged cartilage tissue	158:181	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	8	21	theme	navigation	1689:1698	arg1	behavior					1700:1707	cell navigation behavior	1684:1707	cell navigation behavior	1684:1707	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	4	22	theme	hyaluronic	946:955	arg1	HA					963:964	HA	963:964	HA	963:964	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	4	22	theme	hyaluronic	946:955	arg1	acid					957:960	hyaluronic acid	946:960	hyaluronic acid (HA)	946:965	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	4	23	theme	hydrogel	1084:1091	arg1	self-healing					1057:1068	the self-healing	1053:1068	the self-healing of the HA-pAA hydrogel at the injured site	1053:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	5	24	theme	HA	1225:1226	arg1	chains					1236:1241	the HA polymer chains	1221:1241	the HA polymer chains of LbL-PPMMs toward the surface of the damaged site	1221:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	5	25	theme	embedded	1144:1151	arg1	chondrocytes					1131:1142	the chondrocytes	1127:1142	the chondrocytes embedded in the HA-pAA hydrogel	1127:1174	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	7	26	theme	cell	1621:1624	arg1	arrangement					1626:1636	the cell arrangement	1617:1636	the cell arrangement in normal cartilage tissue	1617:1663	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	9	27	theme	system	1916:1921	arg1	potential					1872:1880	the highly promising potential	1851:1880	the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair	1851:1948	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	8	28	from	hydrogel	1750:1757	arg1	behavior					1700:1707	cell navigation behavior	1684:1707	cell navigation behavior	1684:1707	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	8	28	from	hydrogel	1750:1757	arg1	release					1717:1723	GSH release	1713:1723	GSH release	1713:1723	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	3	29	theme	iron	821:824	arg1	oxide					826:830	iron oxide	821:830	iron oxide	821:830	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	0	30	theme	glutathione	78:88	arg1	release					90:96	controlled glutathione release	67:96	controlled glutathione release	67:96	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	3	31	theme	located	851:857	arg1	IO					847:848	IO	847:848	IO	847:848	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	31	theme	located	851:857	arg1	nanoparticles					832:844	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles	712:844	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell	712:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	4	32	theme	LbL	929:931	arg1	assembly					934:941	layer-by-layer (LbL) assembly	913:941	layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs)	913:985	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	9	33	theme	cartilage	1926:1934	arg1	repair					1943:1948	cartilage tissue repair	1926:1948	cartilage tissue repair	1926:1948	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	5	34	theme	CD44	1203:1206	arg1	receptors					1208:1216	CD44 receptors	1203:1216	CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site	1203:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	0	35	dep	in	112:113	arg1	situ					115:118	situ	115:118	situ	115:118	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	0	36	theme	in	112:113	arg1	array					132:136	in situ chondrocyte array	112:136	in situ chondrocyte array	112:136	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	7	37	theme	columnar	1590:1597	arg1	array					1599:1603	a columnar array	1588:1603	a columnar array	1588:1603	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	6	38	theme	chondrocytes/LbL-PPMMs/HA-pAA	1359:1387	arg1	system					1349:1354	The composite hydrogel system	1326:1354	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA	1326:1387	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	0	39	theme	injectable	8:17	arg1	hydrogel					29:36	A smart injectable composite hydrogel	0:36	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.	0:182	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	3	40	dep	poly	719:722	arg1	PPMMs					780:784	PPMMs	780:784	PPMMs	780:784	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	40	dep	poly	719:722	arg1	microcapsules					765:777	magnetic microcapsules	756:777	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell	712:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	7	41	theme	zone	1568:1571	arg1	tissue					1573:1578	the deep zone tissue	1559:1578	the deep zone tissue	1559:1578	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	2	42	theme	acid	497:500	arg1	hydrogel					511:518	an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel	455:518	an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel	455:518	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	5	43	theme	LbL-PPMMs	1246:1254	arg1	chains					1236:1241	the HA polymer chains	1221:1241	the HA polymer chains of LbL-PPMMs toward the surface of the damaged site	1221:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	6	44	theme	composite	1330:1338	arg1	system					1349:1354	The composite hydrogel system	1326:1354	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA	1326:1387	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	2	45	theme	hyaluronic	469:478	arg1	HA-pAA					503:508	HA-pAA	503:508	HA-pAA	503:508	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	2	45	theme	hyaluronic	469:478	arg1	acid					497:500	hyaluronic acid-polyacrylic acid	469:500	an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel	455:518	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	5	46	theme	internal	1301:1308	arg1	force					1319:1323	an internal magnetic force	1298:1323	an internal magnetic force	1298:1323	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	0	47	theme	magnetic	43:50	arg1	navigation					52:61	magnetic navigation	43:61	magnetic navigation	43:61	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	4	48	from	site	1108:1111	arg1	self-healing					1057:1068	the self-healing	1053:1068	the self-healing of the HA-pAA hydrogel at the injured site	1053:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	4	49	theme	slow	1021:1024	arg1	release					1026:1032	a two-stage rapid and slow release	999:1032	a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site	999:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	9	50	theme	LbL-PPMM/HA-pAA	1900:1914	arg1	system					1916:1921	the injectable LbL-PPMM/HA-pAA system	1885:1921	the injectable LbL-PPMM/HA-pAA system	1885:1921	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	7	51	from	arrangement	1626:1636	arg1	tissue					1658:1663	normal cartilage tissue	1641:1663	normal cartilage tissue	1641:1663	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	5	52	theme	damaged	1282:1288	arg1	site					1290:1293	the damaged site	1278:1293	the damaged site	1278:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	1	53	theme	invasive	256:263	arg1	way					265:267	a minimally invasive way	244:267	a minimally invasive way for articular cartilage lesions	244:299	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	4	54	theme	GSH	971:973	arg1	assembly					934:941	layer-by-layer (LbL) assembly	913:941	layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs)	913:985	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	9	55	theme	injectable	1889:1898	arg1	system					1916:1921	the injectable LbL-PPMM/HA-pAA system	1885:1921	the injectable LbL-PPMM/HA-pAA system	1885:1921	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	0	56	with	hydrogel	29:36	arg1	release					90:96	controlled glutathione release	67:96	controlled glutathione release	67:96	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	0	56	with	hydrogel	29:36	arg1	navigation					52:61	magnetic navigation	43:61	magnetic navigation	43:61	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	6	57	theme	damaged	1405:1411	arg1	cartilage					1413:1421	the damaged cartilage	1401:1421	the damaged cartilage with a more even and smooth surface than other groups in a rabbit model	1401:1493	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	4	58	theme	rapid	1011:1015	arg1	release					1026:1032	a two-stage rapid and slow release	999:1032	a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site	999:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	3	59	theme	porous	712:717	arg1	poly					719:722	porous poly	712:722	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell	712:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	59	theme	porous	712:717	arg1	acid					743:746	lactic-co-glycolic acid	724:746	lactic-co-glycolic acid	724:746	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	59	theme	porous	712:717	arg1	PLGA					750:753	PLGA	750:753	PLGA	750:753	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	8	60	theme	lesions	1778:1784	arg1	closure					1767:1773	a full closure	1760:1773	a full closure of lesions on the cartilage tissue	1760:1808	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	1	61	theme	cartilage	283:291	arg1	lesions					293:299	articular cartilage lesions	273:299	articular cartilage lesions	273:299	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	1	62	from	chondrocytes	309:320	arg1	hydrogels					340:348	the injectable hydrogels	325:348	the injectable hydrogels	325:348	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	3	63	from	shell	866:870	arg1	located					851:857	located	851:857	located	851:857	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	4	64	theme	HA-pAA	1077:1082	arg1	hydrogel					1084:1091	the HA-pAA hydrogel	1073:1091	the HA-pAA hydrogel	1073:1091	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	6	65	with	cartilage	1413:1421	arg1	surface					1451:1457	a more even and smooth surface	1428:1457	a more even and smooth surface than other groups	1428:1475	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	6	66	theme	other	1464:1468	arg1	groups					1470:1475	other groups	1464:1475	other groups	1464:1475	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	1	67	theme	cell-based	195:204	arg1	hydrogels					206:214	Injectable cell-based hydrogels	184:214	Injectable cell-based hydrogels	184:214	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	5	68	from	embedded	1144:1151	arg1	hydrogel					1167:1174	the HA-pAA hydrogel	1156:1174	the HA-pAA hydrogel	1156:1174	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	4	69	theme	GSH-loaded	891:900	arg1	PPMMs					902:906	The GSH-loaded PPMMs	887:906	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs)	887:985	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	2	70	theme	hyaluronic	548:557	arg1	HA-CD					578:582	HA-CD	578:582	HA-CD	578:582	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	2	70	theme	hyaluronic	548:557	arg1	acid-cyclodextrin					559:575	hyaluronic acid-cyclodextrin	548:575	hyaluronic acid-cyclodextrin (HA-CD)	548:583	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	5	71	from	hydrogel	1167:1174	arg1	embedded					1144:1151	embedded	1144:1151	embedded	1144:1151	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	6	72	theme	implantation	1512:1523	arg1	weeks					1503:1507	8 weeks	1501:1507	8 weeks of implantation	1501:1523	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	2	73	theme	polyacrylic	589:599	arg1	pAA-Fc					617:622	pAA-Fc	617:622	pAA-Fc	617:622	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	2	73	theme	polyacrylic	589:599	arg1	acid-ferrocene					601:614	polyacrylic acid-ferrocene	589:614	polyacrylic acid-ferrocene (pAA-Fc)	589:623	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	0	74	theme	controlled	67:76	arg1	release					90:96	controlled glutathione release	67:96	controlled glutathione release	67:96	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	8	75	theme	cell	1684:1687	arg1	behavior					1700:1707	cell navigation behavior	1684:1707	cell navigation behavior	1684:1707	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	7	76	theme	normal	1641:1646	arg1	tissue					1658:1663	normal cartilage tissue	1641:1663	normal cartilage tissue	1641:1663	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	4	77	with	PPMMs	902:906	arg1	assembly					934:941	layer-by-layer (LbL) assembly	913:941	layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs)	913:985	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	4	78	theme	injured	1100:1106	arg1	site					1108:1111	the injured site	1096:1111	the injured site	1096:1111	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	6	79	theme	rabbit	1482:1487	arg1	model					1489:1493	a rabbit model	1480:1493	a rabbit model	1480:1493	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	4	80	theme	acid	957:960	arg1	assembly					934:941	layer-by-layer (LbL) assembly	913:941	layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs)	913:985	The GSH-loaded PPMMs with layer-by-layer (LbL) assembly of hyaluronic acid (HA) and GSH (LbL-PPMMs) can provide a two-stage rapid and slow release of GSH to modulate the self-healing of the HA-pAA hydrogel at the injured site.					
34726686	8	81	theme	GSH	1713:1715	arg1	release					1717:1723	GSH release	1713:1723	GSH release	1713:1723	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	9	82	theme	tissue	1936:1941	arg1	repair					1943:1948	cartilage tissue repair	1926:1948	cartilage tissue repair	1926:1948	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	7	83	from	chondrocytes	1543:1554	arg1	tissue					1573:1578	the deep zone tissue	1559:1578	the deep zone tissue	1559:1578	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	7	84	theme	similar	1606:1612	arg1	array					1599:1603	a columnar array	1588:1603	a columnar array	1588:1603	In addition, the chondrocytes in the deep zone tissue exhibit a columnar array, similar to the cell arrangement in normal cartilage tissue.					
34726686	0	85	theme	smart	2:6	arg1	hydrogel					29:36	A smart injectable composite hydrogel	0:36	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.	0:182	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	8	86	theme	LbL-PPMM/HA-pAA	1734:1748	arg1	hydrogel					1750:1757	the LbL-PPMM/HA-pAA hydrogel	1730:1757	the LbL-PPMM/HA-pAA hydrogel	1730:1757	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	5	87	theme	polymer	1228:1234	arg1	chains					1236:1241	the HA polymer chains	1221:1241	the HA polymer chains of LbL-PPMMs toward the surface of the damaged site	1221:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	0	88	theme	composite	19:27	arg1	hydrogel					29:36	A smart injectable composite hydrogel	0:36	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.	0:182	A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue.					
34726686	3	89	with	microcapsules	765:777	arg1	GSH					805:807	GSH	805:807	GSH	805:807	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	89	with	microcapsules	765:777	arg1	glutathione					792:802	glutathione	792:802	glutathione (GSH) loaded	792:815	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	89	with	microcapsules	765:777	arg1	oxide					826:830	iron oxide	821:830	iron oxide	821:830	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	9	90	from	potential	1872:1880	arg1	repair					1943:1948	cartilage tissue repair	1926:1948	cartilage tissue repair	1926:1948	Our results demonstrate the highly promising potential of the injectable LbL-PPMM/HA-pAA system in cartilage tissue repair.					
34726686	8	91	from	closure	1767:1773	arg1	tissue					1803:1808	the cartilage tissue	1789:1808	the cartilage tissue	1789:1808	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	6	92	theme	hydrogel	1340:1347	arg1	system					1349:1354	The composite hydrogel system	1326:1354	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA	1326:1387	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	2	93	theme	acid-polyacrylic	480:495	arg1	HA-pAA					503:508	HA-pAA	503:508	HA-pAA	503:508	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	2	93	theme	acid-polyacrylic	480:495	arg1	acid					497:500	hyaluronic acid-polyacrylic acid	469:500	an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel	455:518	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	1	94	theme	injectable	329:338	arg1	hydrogels					340:348	the injectable hydrogels	325:348	the injectable hydrogels	325:348	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
34726686	2	95	theme	injectable	458:467	arg1	hydrogel					511:518	an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel	455:518	an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel	455:518	In this study, an injectable hyaluronic acid-polyacrylic acid (HA-pAA) hydrogel was first synthesized using hyaluronic acid-cyclodextrin (HA-CD) and polyacrylic acid-ferrocene (pAA-Fc) to provide cell-delivery and self-healing.					
34726686	5	96	theme	magnetic	1310:1317	arg1	force					1319:1323	an internal magnetic force	1298:1323	an internal magnetic force	1298:1323	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	6	97	theme	even	1435:1438	arg1	surface					1451:1457	a more even and smooth surface	1428:1457	a more even and smooth surface than other groups	1428:1475	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	8	98	dep	behavior	1700:1707	arg1	the					1680:1682	the	1680:1682	the	1680:1682	Together with the cell navigation behavior and GSH release from the LbL-PPMM/HA-pAA hydrogel, a full closure of lesions on the cartilage tissue can be achieved.					
34726686	3	99	theme	cell	683:686	arg1	fixation					688:695	the cell fixation	679:695	the cell fixation	679:695	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	5	100	theme	HA-pAA	1160:1165	arg1	hydrogel					1167:1174	the HA-pAA hydrogel	1156:1174	the HA-pAA hydrogel	1156:1174	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	6	101	from	cartilage	1413:1421	arg1	model					1489:1493	a rabbit model	1480:1493	a rabbit model	1480:1493	The composite hydrogel system of chondrocytes/LbL-PPMMs/HA-pAA can provide the damaged cartilage with a more even and smooth surface than other groups in a rabbit model after 8 weeks of implantation.					
34726686	3	102	theme	poly	719:722	arg1	IO					847:848	IO	847:848	IO	847:848	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	3	102	theme	poly	719:722	arg1	nanoparticles					832:844	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles	712:844	porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell	712:870	To promote the cell fixation and alignment, porous poly(lactic-co-glycolic acid) (PLGA) magnetic microcapsules (PPMMs) with glutathione (GSH) loaded and iron oxide nanoparticles (IO) located in the shell were designed.					
34726686	5	103	theme	site	1290:1293	arg1	surface					1267:1273	the surface	1263:1273	the surface of the damaged site	1263:1293	Furthermore, the chondrocytes embedded in the HA-pAA hydrogel could be delivered through CD44 receptors on the HA polymer chains of LbL-PPMMs toward the surface of the damaged site by an internal magnetic force.					
34726686	1	104	from	site	391:394	arg1	fixed					378:382	fixed	378:382	fixed	378:382	Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site of interest to repair the cartilage tissue.					
32238136	0	0	theme	Extracts	86:93	arg1	Determination					0:12	Determination	0:12	Determination of Chemical Composition	0:36	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	0	0	theme	Extracts	86:93	arg1	Effect					63:68	α-amylase Inhibitory Effect	42:68	α-amylase Inhibitory Effect of New Propolis Extracts	42:93	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	7	1	theme	Total	784:788	arg1	content					799:805	Total phenolic content	784:805	Total phenolic content	784:805	Total phenolic content and antioxidant activity of the extracts were measured.					
32238136	11	2	theme	same	1264:1267	arg1	compounds					1278:1286	the same phenolic compounds	1260:1286	the same phenolic compounds	1260:1286	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	6	3	theme	METHODS	702:708	arg1	composition					719:729	METHODS Chemical composition	702:729	METHODS Chemical composition of extracts	702:741	METHODS Chemical composition of extracts was determined by using GC-MS equipment.					
32238136	3	4	theme	extraction	493:502	arg1	process					482:488	the process	478:488	the process of extraction	478:502	Propolis can be extracted and the solubility of propolis differs depending on the solvent used in the process of extraction.					
32238136	11	5	theme	volatile	1317:1324	arg1	extract					1330:1336	volatile oil extract	1317:1336	volatile oil extract	1317:1336	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	4	6	theme	propolis	570:577	arg1	extract					579:585	the propolis extract	566:585	the propolis extract	566:585	Solvents used for propolis extraction have a great impact on the propolis extract and should be nontoxic.					
32238136	0	7	theme	Propolis	77:84	arg1	Extracts					86:93	New Propolis Extracts	73:93	New Propolis Extracts	73:93	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	5	8	theme	raw	636:638	arg1	propolis					640:647	raw propolis	636:647	raw propolis	636:647	OBJECTIVE In this study, raw propolis was extracted by peppermint and clove volatile oils.					
32238136	2	9	theme	antiviral	346:354	arg1	effects					371:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	12	10	theme	reference	1437:1445	arg1	acarbose					1458:1465	acarbose	1458:1465	acarbose	1458:1465	Both of these extracts showed better α-amylase ınhibitory activity than a reference inhibitor, acarbose.					
32238136	12	10	theme	reference	1437:1445	arg1	inhibitor					1447:1455	a reference inhibitor	1435:1455	a reference inhibitor	1435:1455	Both of these extracts showed better α-amylase ınhibitory activity than a reference inhibitor, acarbose.					
32238136	11	11	theme	phenolic	1269:1276	arg1	compounds					1278:1286	the same phenolic compounds	1260:1286	the same phenolic compounds	1260:1286	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	13	12	theme	volatile	1541:1548	arg1	oils					1550:1553	volatile oils	1541:1553	volatile oils	1541:1553	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	9	13	theme	propolis	1046:1053	arg1	extraction					1032:1041	the extraction	1028:1041	the extraction of propolis	1028:1053	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	10	14	theme	clove	1179:1183	arg1	extracts					1213:1220	clove and peppermint oil propolis extracts	1179:1220	clove and peppermint oil propolis extracts	1179:1220	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	15	theme	phenolic	1095:1102	arg1	content					1104:1110	The total phenolic content	1085:1110	The total phenolic content of these extracts	1085:1128	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	15	theme	phenolic	1095:1102	arg1	GAE/mL					1168:1173	40.80 mg GAE/mL	1159:1173	40.80 mg GAE/mL	1159:1173	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	15	theme	phenolic	1095:1102	arg1	175.12					1148:1153	175.12	1148:1153	175.12	1148:1153	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	13	16	theme	complementary	1574:1586	arg1	extract					1515:1521	propolis extract	1506:1521	propolis extract obtained by using volatile oils	1506:1553	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	13	16	theme	complementary	1574:1586	arg1	agent					1588:1592	a complementary agent	1572:1592	a complementary agent in the treatment of type 2 diabetes mellitus	1572:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	4	17	contain	have	543:546	arg2	impact					556:561	a great impact	548:561	a great impact	548:561	Solvents used for propolis extraction have a great impact on the propolis extract and should be nontoxic.					
32238136	4	17	contain	have	543:546	arg1	Solvents					505:512	Solvents	505:512	Solvents used for propolis extraction	505:541	Solvents used for propolis extraction have a great impact on the propolis extract and should be nontoxic.					
32238136	8	18	theme	inhibitory	873:882	arg1	activity					884:891	α-amylase inhibitory activity	863:891	α-amylase inhibitory activity of the extracts	863:907	α-amylase inhibitory activity of the extracts was carried out as well.					
32238136	8	18	theme	inhibitory	873:882	arg1	well					928:931	well	928:931	well	928:931	α-amylase inhibitory activity of the extracts was carried out as well.					
32238136	10	19	theme	total	1089:1093	arg1	content					1104:1110	The total phenolic content	1085:1110	The total phenolic content of these extracts	1085:1128	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	19	theme	total	1089:1093	arg1	GAE/mL					1168:1173	40.80 mg GAE/mL	1159:1173	40.80 mg GAE/mL	1159:1173	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	19	theme	total	1089:1093	arg1	175.12					1148:1153	175.12	1148:1153	175.12	1148:1153	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	9	20	theme	peppermint	1060:1069	arg1	oil					1080:1082	peppermint volatile oil	1060:1082	peppermint volatile oil	1060:1082	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	8	21	theme	α-amylase	863:871	arg1	activity					884:891	α-amylase inhibitory activity	863:891	α-amylase inhibitory activity of the extracts	863:907	α-amylase inhibitory activity of the extracts was carried out as well.					
32238136	8	21	theme	α-amylase	863:871	arg1	well					928:931	well	928:931	well	928:931	α-amylase inhibitory activity of the extracts was carried out as well.					
32238136	11	22	contain	contained	1250:1258	arg2	compounds					1278:1286	the same phenolic compounds	1260:1286	the same phenolic compounds	1260:1286	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	11	22	contain	contained	1250:1258	arg1	extracts					1241:1248	All extracts	1237:1248	All extracts	1237:1248	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	10	23	theme	mg	1165:1166	arg1	content					1104:1110	The total phenolic content	1085:1110	The total phenolic content of these extracts	1085:1128	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	23	theme	mg	1165:1166	arg1	GAE/mL					1168:1173	40.80 mg GAE/mL	1159:1173	40.80 mg GAE/mL	1159:1173	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	24	theme	peppermint	1189:1198	arg1	extracts					1213:1220	clove and peppermint oil propolis extracts	1179:1220	clove and peppermint oil propolis extracts	1179:1220	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	9	25	from	extraction	1032:1041	arg1	effective					1015:1023	effective	1015:1023	effective	1015:1023	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	7	26	theme	extracts	839:846	arg1	content					799:805	Total phenolic content	784:805	Total phenolic content	784:805	Total phenolic content and antioxidant activity of the extracts were measured.					
32238136	7	26	theme	extracts	839:846	arg1	activity					823:830	antioxidant activity	811:830	antioxidant activity	811:830	Total phenolic content and antioxidant activity of the extracts were measured.					
32238136	7	27	theme	phenolic	790:797	arg1	content					799:805	Total phenolic content	784:805	Total phenolic content	784:805	Total phenolic content and antioxidant activity of the extracts were measured.					
32238136	10	28	theme	extracts	1121:1128	arg1	content					1104:1110	The total phenolic content	1085:1110	The total phenolic content of these extracts	1085:1128	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	28	theme	extracts	1121:1128	arg1	GAE/mL					1168:1173	40.80 mg GAE/mL	1159:1173	40.80 mg GAE/mL	1159:1173	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	28	theme	extracts	1121:1128	arg1	175.12					1148:1153	175.12	1148:1153	175.12	1148:1153	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	9	29	from	effective	1015:1023	arg1	extraction					1032:1041	the extraction	1028:1041	the extraction of propolis	1028:1053	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	12	30	theme	better	1393:1398	arg1	activity					1421:1428	better α-amylase ınhibitory activity	1393:1428	better α-amylase ınhibitory activity	1393:1428	Both of these extracts showed better α-amylase ınhibitory activity than a reference inhibitor, acarbose.					
32238136	9	31	theme	present	962:968	arg1	study					970:974	the present study	958:974	the present study	958:974	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	4	32	theme	great	550:554	arg1	impact					556:561	a great impact	548:561	a great impact	548:561	Solvents used for propolis extraction have a great impact on the propolis extract and should be nontoxic.					
32238136	6	33	theme	GC-MS	767:771	arg1	equipment					773:781	GC-MS equipment	767:781	GC-MS equipment	767:781	METHODS Chemical composition of extracts was determined by using GC-MS equipment.					
32238136	1	34	theme	BACKGROUND	96:105	arg1	mixture					130:136	a resinous mixture	119:136	a resinous mixture collected by honeybees from tree buds and exudates of the plants	119:201	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	1	34	theme	BACKGROUND	96:105	arg1	Propolis					107:114	BACKGROUND Propolis	96:114	BACKGROUND Propolis	96:114	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	8	35	theme	extracts	900:907	arg1	activity					884:891	α-amylase inhibitory activity	863:891	α-amylase inhibitory activity of the extracts	863:907	α-amylase inhibitory activity of the extracts was carried out as well.					
32238136	8	35	theme	extracts	900:907	arg1	well					928:931	well	928:931	well	928:931	α-amylase inhibitory activity of the extracts was carried out as well.					
32238136	3	36	theme	propolis	428:435	arg1	solubility					414:423	the solubility	410:423	the solubility of propolis	410:435	Propolis can be extracted and the solubility of propolis differs depending on the solvent used in the process of extraction.					
32238136	0	37	theme	Composition	26:36	arg1	Determination					0:12	Determination	0:12	Determination of Chemical Composition	0:36	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	0	37	theme	Composition	26:36	arg1	Effect					63:68	α-amylase Inhibitory Effect	42:68	α-amylase Inhibitory Effect of New Propolis Extracts	42:93	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	5	38	theme	clove	681:685	arg1	oils					696:699	peppermint and clove volatile oils	666:699	peppermint and clove volatile oils	666:699	OBJECTIVE In this study, raw propolis was extracted by peppermint and clove volatile oils.					
32238136	11	39	theme	ethanol	1346:1352	arg1	extract					1354:1360	ethanol extract	1346:1360	ethanol extract	1346:1360	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	0	40	theme	Chemical	17:24	arg1	Composition					26:36	Chemical Composition	17:36	Chemical Composition	17:36	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	5	41	from	OBJECTIVE	611:619	arg1	study					629:633	this study	624:633	this study	624:633	OBJECTIVE In this study, raw propolis was extracted by peppermint and clove volatile oils.					
32238136	5	42	theme	volatile	687:694	arg1	oils					696:699	peppermint and clove volatile oils	666:699	peppermint and clove volatile oils	666:699	OBJECTIVE In this study, raw propolis was extracted by peppermint and clove volatile oils.					
32238136	13	43	theme	diabetes	1621:1628	arg1	mellitus					1630:1637	type 2 diabetes mellitus	1614:1637	type 2 diabetes mellitus	1614:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	1	44	theme	tree	166:169	arg1	buds					171:174	tree buds	166:174	tree buds	166:174	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	7	45	theme	antioxidant	811:821	arg1	activity					823:830	antioxidant activity	811:830	antioxidant activity	811:830	Total phenolic content and antioxidant activity of the extracts were measured.					
32238136	1	46	theme	plants	196:201	arg1	exudates					180:187	exudates	180:187	exudates	180:187	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	1	46	theme	plants	196:201	arg1	buds					171:174	tree buds	166:174	tree buds	166:174	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	0	47	theme	α-amylase	42:50	arg1	Effect					63:68	α-amylase Inhibitory Effect	42:68	α-amylase Inhibitory Effect of New Propolis Extracts	42:93	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	2	48	theme	anticancer	334:343	arg1	effects					371:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	13	49	theme	mellitus	1630:1637	arg1	treatment					1601:1609	the treatment	1597:1609	the treatment of type 2 diabetes mellitus	1597:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	12	50	theme	α-amylase	1400:1408	arg1	activity					1421:1428	better α-amylase ınhibitory activity	1393:1428	better α-amylase ınhibitory activity	1393:1428	Both of these extracts showed better α-amylase ınhibitory activity than a reference inhibitor, acarbose.					
32238136	9	51	theme	clove	988:992	arg1	oil					1003:1005	clove volatile oil	988:1005	clove volatile oil	988:1005	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	4	52	theme	propolis	523:530	arg1	extraction					532:541	propolis extraction	523:541	propolis extraction	523:541	Solvents used for propolis extraction have a great impact on the propolis extract and should be nontoxic.					
32238136	9	53	theme	study	970:974	arg1	findings					946:953	The findings	942:953	The findings of the present study	942:974	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	9	54	theme	volatile	994:1001	arg1	oil					1003:1005	clove volatile oil	988:1005	clove volatile oil	988:1005	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	13	55	dep	CONCLUSION	1468:1477	arg1	concluded					1491:1499	concluded	1491:1499	could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus	1482:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	2	56	theme	aromatic	222:229	arg1	acids					231:235	aromatic acids	222:235	aromatic acids	222:235	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	9	57	dep	RESULTS	934:940	arg1	showed					976:981	showed	976:981	showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil	976:1082	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	0	58	theme	Inhibitory	52:61	arg1	Effect					63:68	α-amylase Inhibitory Effect	42:68	α-amylase Inhibitory Effect of New Propolis Extracts	42:93	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	13	59	theme	type	1614:1617	arg1	mellitus					1630:1637	type 2 diabetes mellitus	1614:1637	type 2 diabetes mellitus	1614:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	1	60	theme	resinous	121:128	arg1	mixture					130:136	a resinous mixture	119:136	a resinous mixture collected by honeybees from tree buds and exudates of the plants	119:201	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	1	60	theme	resinous	121:128	arg1	Propolis					107:114	BACKGROUND Propolis	96:114	BACKGROUND Propolis	96:114	BACKGROUND Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants.					
32238136	5	61	theme	peppermint	666:675	arg1	oils					696:699	peppermint and clove volatile oils	666:699	peppermint and clove volatile oils	666:699	OBJECTIVE In this study, raw propolis was extracted by peppermint and clove volatile oils.					
32238136	2	62	theme	phenolic	259:266	arg1	compounds					268:276	phenolic compounds	259:276	phenolic compounds	259:276	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	6	63	theme	extracts	734:741	arg1	composition					719:729	METHODS Chemical composition	702:729	METHODS Chemical composition of extracts	702:741	METHODS Chemical composition of extracts was determined by using GC-MS equipment.					
32238136	12	64	theme	ınhibitory	1410:1419	arg1	activity					1421:1428	better α-amylase ınhibitory activity	1393:1428	better α-amylase ınhibitory activity	1393:1428	Both of these extracts showed better α-amylase ınhibitory activity than a reference inhibitor, acarbose.					
32238136	2	65	contain	contains	213:220	arg2	compounds					268:276	phenolic compounds	259:276	phenolic compounds	259:276	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	2	65	contain	contains	213:220	arg2	acids					249:253	diterpenic acids	238:253	diterpenic acids	238:253	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	2	65	contain	contains	213:220	arg2	acids					231:235	aromatic acids	222:235	aromatic acids	222:235	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	2	65	contain	contains	213:220	arg1	Propolis					204:211	Propolis	204:211	Propolis	204:211	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	13	66	used	used	1564:1567	arg2	agent					1588:1592	a complementary agent	1572:1592	a complementary agent in the treatment of type 2 diabetes mellitus	1572:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	13	66	used	used	1564:1567	arg2	extract					1515:1521	propolis extract	1506:1521	propolis extract obtained by using volatile oils	1506:1553	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	13	67	theme	propolis	1506:1513	arg1	extract					1515:1521	propolis extract	1506:1521	propolis extract obtained by using volatile oils	1506:1553	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	13	67	theme	propolis	1506:1513	arg1	agent					1588:1592	a complementary agent	1572:1592	a complementary agent in the treatment of type 2 diabetes mellitus	1572:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
32238136	2	68	theme	antitumor	323:331	arg1	effects					371:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	10	69	theme	propolis	1204:1211	arg1	extracts					1213:1220	clove and peppermint oil propolis extracts	1179:1220	clove and peppermint oil propolis extracts	1179:1220	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	9	70	theme	volatile	1071:1078	arg1	oil					1080:1082	peppermint volatile oil	1060:1082	peppermint volatile oil	1060:1082	RESULTS The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil.					
32238136	0	71	theme	New	73:75	arg1	Extracts					86:93	New Propolis Extracts	73:93	New Propolis Extracts	73:93	Determination of Chemical Composition and α-amylase Inhibitory Effect of New Propolis Extracts.					
32238136	10	72	theme	oil	1200:1202	arg1	extracts					1213:1220	clove and peppermint oil propolis extracts	1179:1220	clove and peppermint oil propolis extracts	1179:1220	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	10	73	theme	40.80	1159:1163	arg1	mg					1165:1166	mg	1165:1166	mg	1165:1166	The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively.					
32238136	11	74	theme	oil	1326:1328	arg1	extract					1330:1336	volatile oil extract	1317:1336	volatile oil extract	1317:1336	All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract.					
32238136	2	75	theme	diterpenic	238:247	arg1	acids					249:253	diterpenic acids	238:253	diterpenic acids	238:253	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	6	76	theme	Chemical	710:717	arg1	composition					719:729	METHODS Chemical composition	702:729	METHODS Chemical composition of extracts	702:741	METHODS Chemical composition of extracts was determined by using GC-MS equipment.					
32238136	2	77	theme	antifungal	360:369	arg1	effects					371:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	its antitumor, anticancer, antiviral and antifungal effects	319:377	Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects.					
32238136	13	78	from	agent	1588:1592	arg1	treatment					1601:1609	the treatment	1597:1609	the treatment of type 2 diabetes mellitus	1597:1637	CONCLUSION It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.					
33137389	10	0	contain	have	1494:1497	arg1	CMEOs					1459:1463	CMEOs	1459:1463	CMEOs	1459:1463	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	10	0	contain	have	1494:1497	arg1	compounds					1479:1487	promising compounds	1469:1487	promising compounds that have antibiofilm activity against C. albicans	1469:1538	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	10	0	contain	have	1494:1497	arg2	activity					1511:1518	antibiofilm activity	1499:1518	antibiofilm activity against C. albicans	1499:1538	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	5	1	theme	physical	1007:1014	arg1	modifications					1016:1028	any morphological and physical modifications	985:1028	any morphological and physical modifications after EO incorporation	985:1051	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	1	2	theme	EO	231:232	arg1	profile					243:249	the essential oil (EO) release profile	212:249	the essential oil (EO) release profile	212:249	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	2	3	theme	spray-drying	420:431	arg1	method					433:438	the spray-drying method	416:438	the spray-drying method	416:438	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	4	theme	release	559:565	arg1	profile					567:573	release profile	559:573	release profile	559:573	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	7	5	dep	In	1081:1082	arg1	vitro					1084:1088	vitro	1084:1088	vitro	1084:1088	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	5	6	theme	regular	922:928	arg1	distribution					930:941	regular distribution	922:941	regular distribution	922:941	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	1	7	dep	produce	118:124	arg1	evaluate					203:210	evaluate	203:210	evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity	203:286	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	9	8	theme	microparticles	1391:1404	arg1	efficiency					1377:1386	the efficiency	1373:1386	the efficiency of microparticles against Candida biofilm	1373:1428	The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm.					
33137389	1	9	theme	release	235:241	arg1	profile					243:249	the essential oil (EO) release profile	212:249	the essential oil (EO) release profile	212:249	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	2	10	theme	morphological	473:485	arg1	parameters					507:516	CMEO morphological and physicochemical parameters	468:516	CMEO morphological and physicochemical parameters	468:516	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	10	theme	morphological	473:485	arg1	EE					551:552	EE	551:552	EE	551:552	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	3	11	theme	biomass	690:696	arg1	measurement					708:718	biomass formation measurement	690:718	biomass formation measurement	690:718	The planktonic activity was quantified by broth microdilution, and the activity against biofilm was quantified by biomass formation measurement.					
33137389	4	12	theme	main	839:842	arg1	citral					855:860	citral	855:860	citral (83.17%)	855:869	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	12	theme	main	839:842	arg1	citronellol					875:885	citronellol	875:885	citronellol (24.53%)	875:894	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	12	theme	main	839:842	arg1	components					844:853	the main components	835:853	the main components citral (83.17%) and citronellol (24.53%)	835:894	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	10	13	theme	promising	1469:1477	arg1	CMEOs					1459:1463	CMEOs	1459:1463	CMEOs	1459:1463	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	10	13	theme	promising	1469:1477	arg1	compounds					1479:1487	promising compounds	1469:1487	promising compounds that have antibiofilm activity against C. albicans	1469:1538	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	5	14	dep	15 μm	969:973	arg1	to					966:967	to	966:967	to	966:967	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	2	15	theme	CMEO	468:471	arg1	parameters					507:516	CMEO morphological and physicochemical parameters	468:516	CMEO morphological and physicochemical parameters	468:516	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	15	theme	CMEO	468:471	arg1	EE					551:552	EE	551:552	EE	551:552	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	3	16	theme	formation	698:706	arg1	measurement					708:718	biomass formation measurement	690:718	biomass formation measurement	690:718	The planktonic activity was quantified by broth microdilution, and the activity against biofilm was quantified by biomass formation measurement.					
33137389	6	17	dep	39	1076:1077	arg1	to					1073:1074	to	1073:1074	to	1073:1074	EE% ranged from 12 to 39%.					
33137389	8	18	theme	minimum	1209:1215	arg1	concentration					1228:1240	minimum inhibitory concentration	1209:1240	The minimum inhibitory concentration (MIC) values for CMEOs	1205:1263	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	8	18	theme	minimum	1209:1215	arg1	MIC					1243:1245	MIC	1243:1245	MIC	1243:1245	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	10	19	theme	antibiofilm	1499:1509	arg1	activity					1511:1518	antibiofilm activity	1499:1518	antibiofilm activity against C. albicans	1499:1538	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	7	20	theme	release	1090:1096	arg1	tests					1098:1102	In vitro release tests	1081:1102	In vitro release tests	1081:1102	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	7	21	theme	In	1081:1082	arg1	tests					1098:1102	In vitro release tests	1081:1102	In vitro release tests	1081:1102	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	1	22	theme	essential	179:187	arg1	oils					189:192	essential oils	179:192	essential oils (CMEOs)	179:200	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	1	22	theme	essential	179:187	arg1	CMEOs					195:199	CMEOs	195:199	CMEOs	195:199	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	5	23	theme	EO	1036:1037	arg1	incorporation					1039:1051	EO incorporation	1036:1051	EO incorporation	1036:1051	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	8	24	theme	concentration	1228:1240	arg1	lower					1270:1274	lower	1270:1274	lower	1270:1274	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	8	24	theme	concentration	1228:1240	arg1	values					1248:1253	The minimum inhibitory concentration (MIC) values	1205:1253	The minimum inhibitory concentration (MIC) values for CMEOs	1205:1263	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	9	25	theme	higher	1318:1323	arg1	percentage					1349:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm.					
33137389	0	26	theme	Essential	0:8	arg1	oils					10:13	Essential oils	0:13	Essential oils	0:13	Essential oils encapsulated in chitosan microparticles against Candida albicans biofilms.					
33137389	7	27	from	%	1159:1159	arg1	PBS					1172:1174	PBS	1172:1174	PBS	1172:1174	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	7	27	from	%	1159:1159	arg1	media					1184:1188	HCl media	1180:1188	HCl media	1180:1188	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	8	28	theme	inhibitory	1217:1226	arg1	concentration					1228:1240	minimum inhibitory concentration	1209:1240	The minimum inhibitory concentration (MIC) values for CMEOs	1205:1263	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	8	28	theme	inhibitory	1217:1226	arg1	MIC					1243:1245	MIC	1243:1245	MIC	1243:1245	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	2	29	theme	essential	346:354	arg1	LEO					361:363	LEO	361:363	LEO	361:363	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	29	theme	essential	346:354	arg1	oil					356:358	lemongrass essential oil	335:358	lemongrass essential oil (LEO)	335:364	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	30	theme	EO	522:523	arg1	efficiency					539:548	EO encapsulation efficiency	522:548	EO encapsulation efficiency	522:548	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	4	31	theme	LEO	725:727	arg1	compositions					737:748	The LEO and GEO compositions	721:748	compositions	737:748	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	2	32	theme	essential	379:387	arg1	GEO					394:396	GEO	394:396	GEO	394:396	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	32	theme	essential	379:387	arg1	oil					389:391	geranium essential oil	370:391	geranium essential oil (GEO)	370:397	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	7	33	dep	EO	1121:1122	arg1	%					1159:1159	33%	1157:1159	33%	1157:1159	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	7	33	dep	EO	1121:1122	arg1	%					1167:1167	55%	1165:1167	55% in PBS and HCl media	1165:1188	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	1	34	theme	study	105:109	arg1	aim					94:96	The aim	90:96	The aim of the study	90:109	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	2	35	theme	geranium	370:377	arg1	GEO					394:396	GEO	394:396	GEO	394:396	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	35	theme	geranium	370:377	arg1	oil					389:391	geranium essential oil	370:391	geranium essential oil (GEO)	370:397	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	4	36	dep	components	844:853	arg1	citral					855:860	citral	855:860	citral (83.17%)	855:869	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	36	dep	components	844:853	arg1	%					868:868	83.17%	863:868	83.17%	863:868	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	36	dep	components	844:853	arg1	citronellol					875:885	citronellol	875:885	citronellol (24.53%)	875:894	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	36	dep	components	844:853	arg1	%					893:893	24.53%	888:893	24.53%	888:893	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	36	dep	components	844:853	arg1	components					844:853	the main components	835:853	the main components citral (83.17%) and citronellol (24.53%)	835:894	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	9	37	theme	Candida	1414:1420	arg1	biofilm					1422:1428	Candida biofilm	1414:1428	Candida biofilm	1414:1428	The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm.					
33137389	3	38	theme	broth	618:622	arg1	microdilution					624:636	broth microdilution	618:636	broth microdilution	618:636	The planktonic activity was quantified by broth microdilution, and the activity against biofilm was quantified by biomass formation measurement.					
33137389	5	39	theme	spherical	947:955	arg1	shape					957:961	spherical shape	947:961	spherical shape (1 to 15 μm)	947:974	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	5	39	theme	spherical	947:955	arg1	15 μm					969:973	15 μm	969:973	15 μm	969:973	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	7	40	theme	HCl	1180:1182	arg1	media					1184:1188	HCl media	1180:1188	HCl media	1180:1188	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	0	41	theme	chitosan	31:38	arg1	microparticles					40:53	chitosan microparticles	31:53	chitosan microparticles	31:53	Essential oils encapsulated in chitosan microparticles against Candida albicans biofilms.					
33137389	1	42	theme	anti-Candida	266:277	arg1	activity					279:286	the CMEOs' anti-Candida activity	255:286	the CMEOs' anti-Candida activity	255:286	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	2	43	theme	physicochemical	491:505	arg1	parameters					507:516	CMEO morphological and physicochemical parameters	468:516	CMEO morphological and physicochemical parameters	468:516	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	43	theme	physicochemical	491:505	arg1	EE					551:552	EE	551:552	EE	551:552	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	5	44	theme	morphological	989:1001	arg1	modifications					1016:1028	any morphological and physical modifications	985:1028	any morphological and physical modifications after EO incorporation	985:1051	The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 μm), without any morphological and physical modifications after EO incorporation.					
33137389	10	45	theme	C.	1528:1529	arg1	albicans					1531:1538	C. albicans	1528:1538	C. albicans	1528:1538	These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans.					
33137389	0	46	theme	Candida	63:69	arg1	albicans					71:78	Candida albicans	63:78	Candida albicans biofilms	63:87	Essential oils encapsulated in chitosan microparticles against Candida albicans biofilms.					
33137389	8	47	theme	pure	1293:1296	arg1	P < 0.05					1303:1310	P < 0.05	1303:1310	P < 0.05	1303:1310	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	8	47	theme	pure	1293:1296	arg1	EOs					1298:1300	pure EOs	1293:1300	pure EOs (P < 0.05)	1293:1311	The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05).					
33137389	7	48	from	%	1167:1167	arg1	PBS					1172:1174	PBS	1172:1174	PBS	1172:1174	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	7	48	from	%	1167:1167	arg1	media					1184:1188	HCl media	1180:1188	HCl media	1180:1188	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	9	49	theme	biofilm	1330:1336	arg1	percentage					1349:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm.					
33137389	4	50	theme	mass	800:803	arg1	GC/MS					819:823	GC/MS	819:823	GC/MS	819:823	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	4	50	theme	mass	800:803	arg1	spectrometry					805:816	mass spectrometry	800:816	mass spectrometry (GC/MS)	800:824	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	6	51	theme	EE	1054:1055	arg1	%					1056:1056	EE%	1054:1056	EE%	1054:1056	EE% ranged from 12 to 39%.					
33137389	3	52	theme	planktonic	580:589	arg1	activity					591:598	The planktonic activity	576:598	The planktonic activity	576:598	The planktonic activity was quantified by broth microdilution, and the activity against biofilm was quantified by biomass formation measurement.					
33137389	9	53	theme	CMEO	1325:1328	arg1	percentage					1349:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm.					
33137389	2	54	theme	encapsulation	525:537	arg1	efficiency					539:548	EO encapsulation efficiency	522:548	EO encapsulation efficiency	522:548	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	4	55	theme	GEO	733:735	arg1	compositions					737:748	The LEO and GEO compositions	721:748	compositions	737:748	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	2	56	theme	chitosan	293:300	arg1	CMs					318:320	CMs	318:320	CMs	318:320	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	56	theme	chitosan	293:300	arg1	microparticles					302:315	The chitosan microparticles	289:315	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO)	289:397	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	1	57	theme	essential	216:224	arg1	profile					243:249	the essential oil (EO) release profile	212:249	the essential oil (EO) release profile	212:249	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	7	58	theme	release	1124:1130	arg1	rates					1132:1136	release rates	1124:1136	release rates	1124:1136	In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively.					
33137389	9	59	theme	inhibition	1338:1347	arg1	percentage					1349:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage	1314:1358	The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm.					
33137389	2	60	theme	lemongrass	335:344	arg1	LEO					361:363	LEO	361:363	LEO	361:363	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	2	60	theme	lemongrass	335:344	arg1	oil					356:358	lemongrass essential oil	335:358	lemongrass essential oil (LEO)	335:364	The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile.					
33137389	1	61	theme	oil	226:228	arg1	profile					243:249	the essential oil (EO) release profile	212:249	the essential oil (EO) release profile	212:249	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
33137389	0	62	theme	albicans	71:78	arg1	biofilms					80:87	Candida albicans biofilms	63:87	Candida albicans biofilms	63:87	Essential oils encapsulated in chitosan microparticles against Candida albicans biofilms.					
33137389	4	63	theme	gas	767:769	arg1	chromatography					771:784	gas chromatography	767:784	gas chromatography combined with mass spectrometry (GC/MS)	767:824	The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%).					
33137389	1	64	theme	chitosan	143:150	arg1	microparticles					152:165	chitosan microparticles	143:165	chitosan microparticles loaded with essential oils (CMEOs)	143:200	The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity.					
32454108	3	0	theme	Electron	470:477	arg1	Microscopy					479:488	Transmission Electron Microscopy	457:488	Transmission Electron Microscopy (TEM)	457:494	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	0	theme	Electron	470:477	arg1	TEM					491:493	TEM	491:493	TEM	491:493	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	8	1	theme	potential	1345:1353	arg1	usage					1355:1359	potential usage	1345:1359	potential usage in nanocomposites for biomedical and food packaging application	1345:1423	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	4	2	theme	X-ray	627:631	arg1	XRD					649:651	XRD	649:651	XRD	649:651	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	4	2	theme	X-ray	627:631	arg1	diffractometer					633:646	X-ray diffractometer	627:646	X-ray diffractometer (XRD)	627:652	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	1	3	theme	fibre	243:247	arg1	waste					230:234	the waste	226:234	the waste bamboo fibre	226:247	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	3	4	theme	Atomic	500:505	arg1	AFM					525:527	AFM	525:527	AFM	525:527	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	4	theme	Atomic	500:505	arg1	Microscopy					513:522	Atomic Force Microscopy	500:522	Atomic Force Microscopy (AFM)	500:528	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	5	theme	extracted	365:373	arg1	's					378:379	The extracted CNC's	361:379	The extracted CNC's	361:379	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	7	6	theme	Acid	1105:1108	arg1	hydrolysis					1110:1119	Acid hydrolysis	1105:1119	Acid hydrolysis for 45 min	1105:1130	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	7	7	theme	higher	1146:1151	arg1	degree					1153:1158	higher degree	1146:1158	higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively	1146:1235	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	3	8	theme	Force	507:511	arg1	AFM					525:527	AFM	525:527	AFM	525:527	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	8	theme	Force	507:511	arg1	Microscopy					513:522	Atomic Force Microscopy	500:522	Atomic Force Microscopy (AFM)	500:528	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	4	9	theme	energy	535:540	arg1	EDX					561:563	EDX	561:563	EDX	561:563	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	4	9	theme	energy	535:540	arg1	X-rays					553:558	The energy Dispersive X-rays	531:558	The energy Dispersive X-rays (EDX)	531:564	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	6	10	theme	needle	1045:1050	arg1	CNC					1059:1061	needle shaped CNC's	1045:1063	needle shaped CNC's	1045:1063	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	6	11	theme	carbon	1000:1005	arg1	content					1007:1013	higher carbon content	993:1013	higher carbon content	993:1013	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	8	12	theme	packaging	1403:1411	arg1	application					1413:1423	biomedical and food packaging application	1383:1423	biomedical and food packaging application	1383:1423	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	5	13	dep	Infrared	744:751	arg1	FTIR					754:757	FTIR	754:757	FTIR	754:757	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	6	14	theme	higher	993:998	arg1	content					1007:1013	higher carbon content	993:1013	higher carbon content	993:1013	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	8	15	theme	food	1398:1401	arg1	application					1413:1423	biomedical and food packaging application	1383:1423	biomedical and food packaging application	1383:1423	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	6	16	with	analysis	979:986	arg1	content					1007:1013	higher carbon content	993:1013	higher carbon content	993:1013	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	2	17	from	isolation	325:333	arg1	fibre					354:358	fibre	354:358	fibre	354:358	In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre.					
32454108	8	18	theme	higher	1247:1252	arg1	quality					1254:1260	higher quality	1247:1260	higher quality of CNC's obtained as a result of efficient and modified techniques	1247:1327	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	8	19	theme	biomedical	1383:1392	arg1	application					1413:1423	biomedical and food packaging application	1383:1423	biomedical and food packaging application	1383:1423	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	4	20	theme	CNC	617:619	arg1	's					620:621	the prepared CNC's	604:621	the prepared CNC's	604:621	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	0	21	theme	Morphological	0:12	arg1	analysis					36:43	Morphological, chemical and thermal analysis	0:43	Morphological, chemical and thermal analysis of cellulose nanocrystals	0:69	Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.					
32454108	6	22	theme	morphological	896:908	arg1	investigations					910:923	their morphological investigations	890:923	their morphological investigations	890:923	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	4	23	theme	prepared	608:615	arg1	's					620:621	the prepared CNC's	604:621	the prepared CNC's	604:621	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	5	24	theme	thermal	771:777	arg1	analysis					779:786	their thermal analysis	765:786	their thermal analysis	765:786	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	6	25	theme	SEM	975:977	arg1	analysis					979:986	SEM analysis	975:986	SEM analysis with higher carbon content	975:1013	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	0	26	theme	chemical	15:22	arg1	analysis					36:43	Morphological, chemical and thermal analysis	0:43	Morphological, chemical and thermal analysis of cellulose nanocrystals	0:69	Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.					
32454108	8	27	theme	modified	1309:1316	arg1	techniques					1318:1327	efficient and modified techniques	1295:1327	efficient and modified techniques	1295:1327	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	6	28	theme	shaped	1052:1057	arg1	CNC					1059:1061	needle shaped CNC's	1045:1063	needle shaped CNC's	1045:1063	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	7	29	theme	CNC	1197:1199	arg1	crystallinity					1163:1175	crystallinity	1163:1175	crystallinity	1163:1175	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	7	29	theme	CNC	1197:1199	arg1	yield					1188:1192	higher yield	1181:1192	higher yield of CNC's about 86.96% and 22% respectively	1181:1235	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	5	30	theme	Differential	841:852	arg1	DSC					876:878	DSC	876:878	DSC	876:878	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	5	30	theme	Differential	841:852	arg1	calorimetry					863:873	Differential scanning calorimetry	841:873	Differential scanning calorimetry (DSC)	841:879	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	1	31	theme	alternative	194:204	arg1	extraction					127:136	the extraction	123:136	the extraction of cellulose nanocrystals (CNC) from bamboo fibre	123:186	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	1	31	theme	alternative	194:204	arg1	approach					206:213	an alternative approach	191:213	an alternative approach to utilize the waste bamboo fibre	191:247	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	8	32	theme	's	1268:1269	arg1	quality					1254:1260	higher quality	1247:1260	higher quality of CNC's obtained as a result of efficient and modified techniques	1247:1327	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	5	33	theme	Thermogravimetric	804:820	arg1	TGA					832:834	TGA	832:834	TGA	832:834	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	5	33	theme	Thermogravimetric	804:820	arg1	Analysis					822:829	Thermogravimetric Analysis	804:829	Thermogravimetric Analysis (TGA)	804:835	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	3	34	theme	Scanning	421:428	arg1	SEM					451:453	SEM	451:453	SEM	451:453	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	34	theme	Scanning	421:428	arg1	Microscopy					439:448	Scanning Electron Microscopy	421:448	Scanning Electron Microscopy (SEM)	421:454	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	0	35	theme	thermal	28:34	arg1	analysis					36:43	Morphological, chemical and thermal analysis	0:43	Morphological, chemical and thermal analysis of cellulose nanocrystals	0:69	Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.					
32454108	8	36	theme	CNC	1265:1267	arg1	's					1268:1269	CNC's	1265:1269	CNC's obtained as a result of efficient and modified techniques	1265:1327	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	2	37	from	fibre	354:358	arg1	CNC					338:340	CNC	338:340	CNC from bamboo fibre	338:358	In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre.					
32454108	2	37	from	fibre	354:358	arg1	isolation					325:333	efficient isolation	315:333	efficient isolation of CNC from bamboo fibre	315:358	In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre.					
32454108	3	38	theme	Electron	430:437	arg1	SEM					451:453	SEM	451:453	SEM	451:453	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	38	theme	Electron	430:437	arg1	Microscopy					439:448	Scanning Electron Microscopy	421:448	Scanning Electron Microscopy (SEM)	421:454	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	0	39	theme	cellulose	48:56	arg1	nanocrystals					58:69	cellulose nanocrystals	48:69	cellulose nanocrystals	48:69	Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.					
32454108	8	40	theme	techniques	1318:1327	arg1	result					1285:1290	a result	1283:1290	a result of efficient and modified techniques	1283:1327	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	2	41	theme	efficient	315:323	arg1	isolation					325:333	efficient isolation	315:333	efficient isolation of CNC from bamboo fibre	315:358	In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre.					
32454108	3	42	theme	CNC	375:377	arg1	's					378:379	The extracted CNC's	361:379	The extracted CNC's	361:379	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	4	43	theme	Dispersive	542:551	arg1	EDX					561:563	EDX	561:563	EDX	561:563	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	4	43	theme	Dispersive	542:551	arg1	X-rays					553:558	The energy Dispersive X-rays	531:558	The energy Dispersive X-rays (EDX)	531:564	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	1	44	theme	nanocrystals	151:162	arg1	extraction					127:136	the extraction	123:136	the extraction of cellulose nanocrystals (CNC) from bamboo fibre	123:186	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	1	44	theme	nanocrystals	151:162	arg1	approach					206:213	an alternative approach	191:213	an alternative approach to utilize the waste bamboo fibre	191:247	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	7	45	theme	higher	1181:1186	arg1	yield					1188:1192	higher yield	1181:1192	higher yield of CNC's about 86.96% and 22% respectively	1181:1235	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	3	46	theme	Transmission	457:468	arg1	Microscopy					479:488	Transmission Electron Microscopy	457:488	Transmission Electron Microscopy (TEM)	457:494	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	3	46	theme	Transmission	457:468	arg1	TEM					491:493	TEM	491:493	TEM	491:493	The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).					
32454108	4	47	theme	's	620:621	arg1	composition					589:599	the elemental composition	575:599	the elemental composition of the prepared CNC's	575:621	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	5	48	theme	scanning	854:861	arg1	DSC					876:878	DSC	876:878	DSC	876:878	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	5	48	theme	scanning	854:861	arg1	calorimetry					863:873	Differential scanning calorimetry	841:873	Differential scanning calorimetry (DSC)	841:879	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	7	49	theme	yield	1188:1192	arg1	degree					1153:1158	higher degree	1146:1158	higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively	1146:1235	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	2	50	theme	acid	295:298	arg1	hydrolysis					300:309	acid hydrolysis	295:309	acid hydrolysis	295:309	In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre.					
32454108	7	51	theme	crystallinity	1163:1175	arg1	degree					1153:1158	higher degree	1146:1158	higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively	1146:1235	Acid hydrolysis for 45 min resulted into higher degree of crystallinity and higher yield of CNC's about 86.96% and 22% respectively.					
32454108	5	52	theme	physiochemical	689:702	arg1	analysis					704:711	The physiochemical analysis	685:711	The physiochemical analysis	685:711	The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC).					
32454108	4	53	theme	elemental	579:587	arg1	composition					589:599	the elemental composition	575:599	the elemental composition of the prepared CNC's	575:621	The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity.					
32454108	8	54	theme	efficient	1295:1303	arg1	techniques					1318:1327	efficient and modified techniques	1295:1327	efficient and modified techniques	1295:1327	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32454108	1	55	from	fibre	182:186	arg1	extraction					127:136	the extraction	123:136	the extraction of cellulose nanocrystals (CNC) from bamboo fibre	123:186	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	1	55	from	fibre	182:186	arg1	approach					206:213	an alternative approach	191:213	an alternative approach to utilize the waste bamboo fibre	191:247	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	1	56	theme	cellulose	141:149	arg1	nanocrystals					151:162	cellulose nanocrystals	141:162	cellulose nanocrystals (CNC)	141:168	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	1	56	theme	cellulose	141:149	arg1	CNC					165:167	CNC	165:167	CNC	165:167	This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre.					
32454108	0	57	theme	nanocrystals	58:69	arg1	analysis					36:43	Morphological, chemical and thermal analysis	0:43	Morphological, chemical and thermal analysis of cellulose nanocrystals	0:69	Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.					
32454108	6	58	theme	AFM	1092:1094	arg1	studies					1096:1102	AFM studies	1092:1102	AFM studies	1092:1102	As from their morphological investigations, rod like structures of CNC's were observed under SEM analysis with higher carbon content as demonstrated by EDX, while needle shaped CNC's were observed from TEM and AFM studies.					
32454108	2	59	theme	CNC	338:340	arg1	isolation					325:333	efficient isolation	315:333	efficient isolation of CNC from bamboo fibre	315:358	In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre.					
32454108	8	60	from	usage	1355:1359	arg1	nanocomposites					1364:1377	nanocomposites	1364:1377	nanocomposites	1364:1377	Owing to higher quality of CNC's obtained as a result of efficient and modified techniques, these can find potential usage in nanocomposites for biomedical and food packaging application.					
32881164	7	0	link	human-derived	1239:1251	arg1	model					1272:1276	a human-derived consortium biofilm model	1237:1276	a human-derived consortium biofilm model	1237:1276	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	3	1	theme	lac	465:467	arg1	operon					469:474	the lac operon	461:474	the lac operon	461:474	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	8	2	theme	biofilms	1427:1434	arg1	composition					1382:1392	composition	1382:1392	composition	1382:1392	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	8	2	theme	biofilms	1427:1434	arg1	potential					1409:1417	pathogenic potential	1398:1417	pathogenic potential	1398:1417	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	0	3	from	behaviors	14:22	arg1	metabolism					35:44	lactose metabolism	27:44	lactose metabolism by Streptococcus mutans	27:68	Subpopulation behaviors in lactose metabolism by Streptococcus mutans.					
32881164	3	4	theme	operon	469:474	arg1	induction					448:456	induction	448:456	induction of the lac operon	448:474	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	4	5	from	activity	651:658	arg1	GS-5					663:666	GS-5	663:666	GS-5	663:666	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	5	6	theme	potential	940:948	arg1	indicative					922:931	indicative	922:931	indicative	922:931	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	7	7	theme	biofilm	1264:1270	arg1	model					1272:1276	a human-derived consortium biofilm model	1237:1276	a human-derived consortium biofilm model	1237:1276	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	8	8	theme	bet-hedging	1301:1311	arg1	behaviors					1313:1321	bet-hedging behaviors	1301:1321	bet-hedging behaviors in carbohydrate metabolism	1301:1348	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	4	9	theme	more	713:716	arg1	glucose					718:724	substantially more glucose	699:724	substantially more glucose	699:724	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	1	10	theme	glucose	248:254	arg1	glucose					248:254	glucose	248:254	glucose	248:254	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	1	10	theme	glucose	248:254	arg1	amounts					237:243	substantial amounts	225:243	substantial amounts of glucose	225:254	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	5	11	with	strains	853:859	arg1	operon					880:885	an intact lac operon	866:885	an intact lac operon	866:885	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	7	12	theme	high	1199:1202	arg1	degree					1204:1209	a high degree	1197:1209	a high degree of competitive fitness	1197:1232	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	7	13	theme	GS-5	1145:1148	arg1	cheaters					1133:1140	Futher, obligate cheaters	1116:1140	cheaters	1133:1140	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	5	14	theme	intact	869:874	arg1	operon					880:885	an intact lac operon	866:885	an intact lac operon	866:885	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	6	15	theme	engineered	994:1003	arg1	strains					1040:1046	engineered obligate cheating and non-cheating strains	994:1046	engineered obligate cheating and non-cheating strains	994:1046	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	5	16	theme	indicative	922:931	arg1	cultures					912:919	the cultures	908:919	the cultures	908:919	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	5	17	theme	sole	830:833	arg1	carbohydrate					835:846	the sole carbohydrate	826:846	the sole carbohydrate	826:846	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	3	18	theme	gfp	406:408	arg1	fusion					419:424	a gfp reporter fusion	404:424	a gfp reporter fusion	404:424	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	1	19	dep	carbohydrate	129:140	arg1	fructose					154:161	fructose	154:161	fructose	154:161	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	1	19	dep	carbohydrate	129:140	arg1	glucose					143:149	glucose	143:149	glucose	143:149	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	3	20	theme	reporter	410:417	arg1	fusion					419:424	a gfp reporter fusion	404:424	a gfp reporter fusion	404:424	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	8	21	theme	pathogenic	1398:1407	arg1	potential					1409:1417	pathogenic potential	1398:1417	pathogenic potential	1398:1417	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	7	22	theme	human-derived	1239:1251	arg1	model					1272:1276	a human-derived consortium biofilm model	1237:1276	a human-derived consortium biofilm model	1237:1276	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	2	23	theme	mutans	293:298	arg1	strains					300:306	S. mutans strains UA159 and GS-5	290:321	strains	300:306	Here, S. mutans strains UA159 and GS-5 were examined for stochastic behaviors in transcription of the lac operon.					
32881164	4	24	theme	Lower	633:637	arg1	activity					651:658	Lower glucokinase activity	633:658	Lower glucokinase activity in GS-5	633:666	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	5	25	theme	unable	785:790	arg1	Mutants					777:783	Mutants	777:783	Mutants unable to use lactose	777:805	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	2	26	theme	S.	290:291	arg1	strains					300:306	S. mutans strains UA159 and GS-5	290:321	strains	300:306	Here, S. mutans strains UA159 and GS-5 were examined for stochastic behaviors in transcription of the lac operon.					
32881164	6	27	theme	cheating	1014:1021	arg1	strains					1040:1046	engineered obligate cheating and non-cheating strains	994:1046	engineered obligate cheating and non-cheating strains	994:1046	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	0	28	theme	lactose	27:33	arg1	metabolism					35:44	lactose metabolism	27:44	lactose metabolism by Streptococcus mutans	27:68	Subpopulation behaviors in lactose metabolism by Streptococcus mutans.					
32881164	6	29	theme	non-cheating	1027:1038	arg1	strains					1040:1046	engineered obligate cheating and non-cheating strains	994:1046	engineered obligate cheating and non-cheating strains	994:1046	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	4	30	theme	glucose	718:724	arg1	release					688:694	release	688:694	release of substantially more glucose than UA159 and significantly lower lac expression	688:774	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	1	31	theme	growth	190:195	arg1	initiation					176:185	initiation	176:185	initiation of growth	176:195	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	7	32	theme	obligate	1124:1131	arg1	cheaters					1133:1140	Futher, obligate cheaters	1116:1140	cheaters	1133:1140	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	3	33	theme	population	509:518	arg1	fraction					493:500	only a fraction	486:500	only a fraction of the population	486:518	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	2	34	theme	operon	390:395	arg1	transcription					365:377	transcription	365:377	transcription of the lac operon	365:395	Here, S. mutans strains UA159 and GS-5 were examined for stochastic behaviors in transcription of the lac operon.					
32881164	7	35	theme	consortium	1253:1262	arg1	model					1272:1276	a human-derived consortium biofilm model	1237:1276	a human-derived consortium biofilm model	1237:1276	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	5	36	from	cultures	912:919	arg1	present					897:903	present	897:903	present	897:903	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	2	37	theme	lac	386:388	arg1	operon					390:395	the lac operon	382:395	the lac operon	382:395	Here, S. mutans strains UA159 and GS-5 were examined for stochastic behaviors in transcription of the lac operon.					
32881164	6	38	theme	strains	1040:1046	arg1	set					987:989	a set	985:989	a set of engineered obligate cheating and non-cheating strains	985:1046	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	6	38	theme	strains	1040:1046	arg1	strains					1040:1046	engineered obligate cheating and non-cheating strains	994:1046	engineered obligate cheating and non-cheating strains	994:1046	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	5	39	attach	present	897:903	arg1	cultures					912:919	the cultures	908:919	the cultures	908:919	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	5	39	attach	present	897:903	arg2	strains					853:859	strains	853:859	strains with an intact lac operon	853:885	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	7	40	theme	Futher	1116:1121	arg1	cheaters					1133:1140	Futher, obligate cheaters	1116:1140	cheaters	1133:1140	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	3	41	theme	carbohydrate	544:555	arg1	source					557:562	carbohydrate source	544:562	carbohydrate source	544:562	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	1	42	theme	several	206:212	arg1	hours					214:218	several hours	206:218	several hours	206:218	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	5	43	located	present	897:903	arg2	strains					853:859	strains	853:859	strains with an intact lac operon	853:885	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	5	43	located	present	897:903	arg1	cultures					912:919	the cultures	908:919	the cultures	908:919	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	8	44	dep	composition	1382:1392	arg1	the					1378:1380	the	1378:1380	the	1378:1380	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	3	45	theme	prior	526:530	arg1	exposure					532:539	prior exposure	526:539	prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response	526:630	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	2	46	from	behaviors	352:360	arg1	transcription					365:377	transcription	365:377	transcription of the lac operon	365:395	Here, S. mutans strains UA159 and GS-5 were examined for stochastic behaviors in transcription of the lac operon.					
32881164	1	47	theme	preferred	119:127	arg1	carbohydrate					129:140	a preferred carbohydrate	117:140	a preferred carbohydrate (glucose or fructose) to lactose	117:173	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	0	48	theme	Streptococcus	49:61	arg1	mutans					63:68	Streptococcus mutans	49:68	Streptococcus mutans	49:68	Subpopulation behaviors in lactose metabolism by Streptococcus mutans.					
32881164	5	49	theme	lac	876:878	arg1	operon					880:885	an intact lac operon	866:885	an intact lac operon	866:885	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	4	50	theme	lac	761:763	arg1	expression					765:774	significantly lower lac expression	741:774	significantly lower lac expression	741:774	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	7	51	theme	competitive	1214:1224	arg1	fitness					1226:1232	competitive fitness	1214:1232	competitive fitness	1214:1232	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	8	52	from	behaviors	1313:1321	arg1	metabolism					1339:1348	carbohydrate metabolism	1326:1348	carbohydrate metabolism	1326:1348	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	3	53	theme	sub-population	608:621	arg1	response					623:630	the sub-population response	604:630	the sub-population response	604:630	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	4	54	theme	lower	755:759	arg1	expression					765:774	significantly lower lac expression	741:774	significantly lower lac expression	741:774	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	7	55	theme	fitness	1226:1232	arg1	degree					1204:1209	a high degree	1197:1209	a high degree of competitive fitness	1197:1232	Futher, obligate cheaters of GS-5 competed well with the non-cheaters and showed a high degree of competitive fitness in a human-derived consortium biofilm model.					
32881164	8	56	theme	carbohydrate	1326:1337	arg1	metabolism					1339:1348	carbohydrate metabolism	1326:1348	carbohydrate metabolism	1326:1348	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	3	57	theme	response	623:630	arg1	magniture					591:599	the magniture	587:599	the magniture of the sub-population response	587:630	Using a gfp reporter fusion, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with prior exposure to carbohydrate source and strain influencing the magniture of the sub-population response.					
32881164	8	58	theme	oral	1422:1425	arg1	biofilms					1427:1434	oral biofilms	1422:1434	oral biofilms	1422:1434	The results show that bet-hedging behaviors in carbohydrate metabolism may substantially influence the composition and pathogenic potential of oral biofilms.					
32881164	6	59	theme	obligate	1005:1012	arg1	strains					1040:1046	engineered obligate cheating and non-cheating strains	994:1046	engineered obligate cheating and non-cheating strains	994:1046	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	2	60	theme	stochastic	341:350	arg1	behaviors					352:360	stochastic behaviors	341:360	stochastic behaviors in transcription of the lac operon	341:395	Here, S. mutans strains UA159 and GS-5 were examined for stochastic behaviors in transcription of the lac operon.					
32881164	5	61	from	present	897:903	arg1	cultures					912:919	the cultures	908:919	the cultures	908:919	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	6	62	theme	heterogeneous	1090:1102	arg1	population					1104:1113	a heterogeneous population	1088:1113	a heterogeneous population	1088:1113	Utilizing a set of engineered obligate cheating and non-cheating strains, we confirmed that cheating can sustain a heterogeneous population.					
32881164	5	63	theme	population	954:963	arg1	cheating					965:972	population cheating	954:972	population cheating	954:972	Mutants unable to use lactose grew on lactose as the sole carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of the potential for population cheating.					
32881164	4	64	theme	glucokinase	639:649	arg1	activity					651:658	Lower glucokinase activity	633:658	Lower glucokinase activity in GS-5	633:666	Lower glucokinase activity in GS-5 was associated with release of substantially more glucose than UA159 and significantly lower lac expression.					
32881164	1	65	theme	substantial	225:235	arg1	glucose					248:254	glucose	248:254	glucose	248:254	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
32881164	1	65	theme	substantial	225:235	arg1	amounts					237:243	substantial amounts	225:243	substantial amounts of glucose	225:254	When Streptococcus mutans is transferred from a preferred carbohydrate (glucose or fructose) to lactose, initiation of growth can take several hours, and substantial amounts of glucose are released during growth.					
34237369	0	0	theme	alginate-kaolin	83:97	arg1	beads					99:103	sodium alginate-kaolin beads	76:103	sodium alginate-kaolin beads	76:103	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	2	1	theme	dye	471:473	arg1	time					498:501	contact time	490:501	contact time	490:501	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	1	theme	dye	471:473	arg1	temperature					526:536	temperature	526:536	temperature	526:536	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	1	theme	dye	471:473	arg1	pH					504:505	pH	504:505	pH	504:505	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	1	theme	dye	471:473	arg1	speed					549:553	agitation speed	539:553	agitation speed	539:553	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	1	theme	dye	471:473	arg1	concentration					475:487	initial dye concentration	463:487	initial dye concentration	463:487	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	1	theme	dye	471:473	arg1	dosage					518:523	adsorbent dosage	508:523	adsorbent dosage	508:523	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	2	theme	removal	563:569	arg1	efficiency					571:580	dye removal efficiency	559:580	dye removal efficiency	559:580	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	1	3	theme	water	155:159	arg1	bodies					161:166	discharges to water bodies	141:166	discharges to water bodies	141:166	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	0	4	theme	sodium	76:81	arg1	beads					99:103	sodium alginate-kaolin beads	76:103	sodium alginate-kaolin beads	76:103	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	0	5	from	modeling	12:19	arg1	adsorption					62:71	methylene blue adsorption	47:71	methylene blue adsorption	47:71	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	2	6	theme	dye	559:561	arg1	efficiency					571:580	dye removal efficiency	559:580	dye removal efficiency	559:580	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	6	7	theme	useful	1045:1050	arg1	model					1022:1026	The developed model	1008:1026	The developed model	1008:1026	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	6	7	theme	useful	1045:1050	arg1	tool					1052:1055	a useful tool	1043:1055	a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions	1043:1165	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	1	8	theme	dye	260:262	arg1	removal					264:270	dye removal	260:270	dye removal	260:270	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	5	9	theme	removal	957:963	arg1	efficiency					965:974	dye removal efficiency	953:974	dye removal efficiency with R2 = 0.97 and RMSE = 3.59	953:1005	The ANN model is best in predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59.					
34237369	4	10	theme	genetic	860:866	arg1	programming					868:878	genetic programming	860:878	genetic programming	860:878	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	8	11	theme	188.7 mg.g-1	1399:1410	arg1	capacity					1387:1394	a maximum adsorption capacity	1366:1394	a maximum adsorption capacity of 188.7 mg.g-1	1366:1410	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	8	11	theme	188.7 mg.g-1	1399:1410	arg1	efficiency					1341:1350	removal efficiency	1333:1350	removal efficiency of 99.56%	1333:1360	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	2	12	dep	parameters	451:460	arg1	time					498:501	contact time	490:501	contact time	490:501	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	12	dep	parameters	451:460	arg1	temperature					526:536	temperature	526:536	temperature	526:536	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	12	dep	parameters	451:460	arg1	pH					504:505	pH	504:505	pH	504:505	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	12	dep	parameters	451:460	arg1	speed					549:553	agitation speed	539:553	agitation speed	539:553	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	12	dep	parameters	451:460	arg1	concentration					475:487	initial dye concentration	463:487	initial dye concentration	463:487	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	12	dep	parameters	451:460	arg1	dosage					518:523	adsorbent dosage	508:523	adsorbent dosage	508:523	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	13	theme	blue	334:337	arg1	dye					319:321	the basic dye	309:321	the basic dye	309:321	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	6	14	theme	basic	1133:1137	arg1	dyes					1139:1142	basic dyes	1133:1142	basic dyes from aqueous solutions	1133:1165	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	2	15	theme	sodium	368:373	arg1	beads					391:395	low-cost sodium alginate-kaolin beads	359:395	low-cost sodium alginate-kaolin beads	359:395	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	16	theme	contact	490:496	arg1	concentration					475:487	initial dye concentration	463:487	initial dye concentration	463:487	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	16	theme	contact	490:496	arg1	time					498:501	contact time	490:501	contact time	490:501	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	17	theme	initial	463:469	arg1	time					498:501	contact time	490:501	contact time	490:501	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	17	theme	initial	463:469	arg1	temperature					526:536	temperature	526:536	temperature	526:536	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	17	theme	initial	463:469	arg1	pH					504:505	pH	504:505	pH	504:505	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	17	theme	initial	463:469	arg1	speed					549:553	agitation speed	539:553	agitation speed	539:553	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	17	theme	initial	463:469	arg1	concentration					475:487	initial dye concentration	463:487	initial dye concentration	463:487	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	17	theme	initial	463:469	arg1	dosage					518:523	adsorbent dosage	508:523	adsorbent dosage	508:523	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	1	18	from	color	132:136	arg1	bodies					161:166	discharges to water bodies	141:166	discharges to water bodies	141:166	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	2	19	theme	low-cost	359:366	arg1	beads					391:395	low-cost sodium alginate-kaolin beads	359:395	low-cost sodium alginate-kaolin beads	359:395	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	0	20	from	study	38:42	arg1	adsorption					62:71	methylene blue adsorption	47:71	methylene blue adsorption	47:71	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	4	21	theme	intelligent	827:837	arg1	techniques					848:857	two powerful intelligent modeling techniques	814:857	two powerful intelligent modeling techniques	814:857	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	4	21	theme	intelligent	827:837	arg1	ANN					911:913	ANN	911:913	ANN	911:913	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	5	22	with	efficiency	965:974	arg1	RMSE = 3.59					995:1005	RMSE = 3.59	995:1005	RMSE = 3.59	995:1005	The ANN model is best in predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59.					
34237369	5	22	with	efficiency	965:974	arg1	R2 = 0.97					981:989	R2 = 0.97	981:989	R2 = 0.97	981:989	The ANN model is best in predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59.					
34237369	0	23	theme	Intelligent	0:10	arg1	modeling					12:19	Intelligent modeling	0:19	Intelligent modeling	0:19	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	4	24	theme	powerful	818:825	arg1	techniques					848:857	two powerful intelligent modeling techniques	814:857	two powerful intelligent modeling techniques	814:857	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	4	24	theme	powerful	818:825	arg1	ANN					911:913	ANN	911:913	ANN	911:913	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	1	25	from	regulations	117:127	arg1	color					132:136	color	132:136	color in discharges to water bodies	132:166	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	1	26	dep	bodies	161:166	arg1	to					152:153	to	152:153	to	152:153	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	4	27	theme	modeling	839:846	arg1	techniques					848:857	two powerful intelligent modeling techniques	814:857	two powerful intelligent modeling techniques	814:857	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	4	27	theme	modeling	839:846	arg1	ANN					911:913	ANN	911:913	ANN	911:913	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	0	28	theme	experimental	25:36	arg1	study					38:42	experimental study	25:42	experimental study	25:42	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	5	29	theme	ANN	921:923	arg1	model					925:929	The ANN model	917:929	The ANN model	917:929	The ANN model is best in predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59.					
34237369	5	29	theme	ANN	921:923	arg1	predicting					942:951	predicting	942:951	predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59	942:1005	The ANN model is best in predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59.					
34237369	8	30	theme	removal	1333:1339	arg1	efficiency					1341:1350	removal efficiency	1333:1350	removal efficiency of 99.56%	1333:1360	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	3	31	theme	individual	607:616	arg1	components					618:627	individual components	607:627	individual components	607:627	The composite beads and individual components were characterized by a number of analytical techniques.					
34237369	1	32	theme	tighter	109:115	arg1	regulations					117:127	tighter regulations	109:127	tighter regulations on color in discharges to water bodies	109:166	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	7	33	theme	Freundlich	1249:1258	arg1	isotherm					1260:1267	the Freundlich isotherm	1245:1267	the Freundlich isotherm	1245:1267	Adsorption followed a pseudo-second order kinetics and was best described by the Freundlich isotherm.					
34237369	2	34	theme	adsorbent	508:516	arg1	dosage					518:523	adsorbent dosage	508:523	adsorbent dosage	508:523	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	34	theme	adsorbent	508:516	arg1	concentration					475:487	initial dye concentration	463:487	initial dye concentration	463:487	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	4	35	theme	regression	789:798	arg1	analysis					800:807	regression analysis	789:807	regression analysis	789:807	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	6	36	theme	developed	1012:1020	arg1	model					1022:1026	The developed model	1008:1026	The developed model	1008:1026	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	6	36	theme	developed	1012:1020	arg1	tool					1052:1055	a useful tool	1043:1055	a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions	1043:1165	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	2	37	theme	parameters	451:460	arg1	effect					431:436	the effect	427:436	the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency	427:580	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	8	38	dep	fourfold	1425:1432	arg1	than					1420:1423	than	1420:1423	than	1420:1423	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	8	38	dep	fourfold	1425:1432	arg1	more					1415:1418	more	1415:1418	more	1415:1418	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	8	39	theme	fourfold	1425:1432	arg1	increase					1434:1441	a more than fourfold increase	1413:1441	a more than fourfold increase over kaolin alone	1413:1459	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	2	40	theme	alginate-kaolin	375:389	arg1	beads					391:395	low-cost sodium alginate-kaolin beads	359:395	low-cost sodium alginate-kaolin beads	359:395	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	0	41	theme	blue	57:60	arg1	adsorption					62:71	methylene blue adsorption	47:71	methylene blue adsorption	47:71	Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads.					
34237369	7	42	theme	pseudo-second	1190:1202	arg1	kinetics					1210:1217	a pseudo-second order kinetics	1188:1217	a pseudo-second order kinetics	1188:1217	Adsorption followed a pseudo-second order kinetics and was best described by the Freundlich isotherm.					
34237369	7	43	theme	order	1204:1208	arg1	kinetics					1210:1217	a pseudo-second order kinetics	1188:1217	a pseudo-second order kinetics	1188:1217	Adsorption followed a pseudo-second order kinetics and was best described by the Freundlich isotherm.					
34237369	8	44	theme	adsorption	1376:1385	arg1	capacity					1387:1394	a maximum adsorption capacity	1366:1394	a maximum adsorption capacity of 188.7 mg.g-1	1366:1410	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	6	45	used	used	1035:1038	arg2	tool					1052:1055	a useful tool	1043:1055	a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions	1043:1165	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	6	45	used	used	1035:1038	arg2	model					1022:1026	The developed model	1008:1026	The developed model	1008:1026	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	2	46	theme	agitation	539:547	arg1	concentration					475:487	initial dye concentration	463:487	initial dye concentration	463:487	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	2	46	theme	agitation	539:547	arg1	speed					549:553	agitation speed	539:553	agitation speed	539:553	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	8	47	theme	maximum	1368:1374	arg1	capacity					1387:1394	a maximum adsorption capacity	1366:1394	a maximum adsorption capacity of 188.7 mg.g-1	1366:1410	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	2	48	theme	dye	319:321	arg1	removal					298:304	the removal	294:304	the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads	294:395	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	6	49	theme	aqueous	1149:1155	arg1	solutions					1157:1165	aqueous solutions	1149:1165	aqueous solutions	1149:1165	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	4	50	theme	neural	895:900	arg1	network					902:908	artificial neural network	884:908	artificial neural network	884:908	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	5	51	theme	dye	953:955	arg1	efficiency					965:974	dye removal efficiency	953:974	dye removal efficiency with R2 = 0.97 and RMSE = 3.59	953:1005	The ANN model is best in predicting dye removal efficiency with R2 = 0.97 and RMSE = 3.59.					
34237369	2	52	theme	basic	313:317	arg1	dye					319:321	the basic dye	309:321	the basic dye	309:321	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	8	53	theme	%	1360:1360	arg1	capacity					1387:1394	a maximum adsorption capacity	1366:1394	a maximum adsorption capacity of 188.7 mg.g-1	1366:1410	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	8	53	theme	%	1360:1360	arg1	efficiency					1341:1350	removal efficiency	1333:1350	removal efficiency of 99.56%	1333:1360	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	4	54	theme	artificial	884:893	arg1	network					902:908	artificial neural network	884:908	artificial neural network	884:908	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	3	55	theme	composite	587:595	arg1	beads					597:601	The composite beads	583:601	The composite beads	583:601	The composite beads and individual components were characterized by a number of analytical techniques.					
34237369	6	56	theme	treatment	1069:1077	arg1	processes					1079:1087	treatment processes	1069:1087	treatment processes	1069:1087	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
34237369	8	57	theme	sodium	1305:1310	arg1	alginate					1312:1319	sodium alginate	1305:1319	sodium alginate	1305:1319	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	1	58	theme	reliable	222:229	arg1	technologies					243:254	reliable inexpensive technologies	222:254	reliable inexpensive technologies	222:254	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	2	59	from	effect	431:436	arg1	efficiency					571:580	dye removal efficiency	559:580	dye removal efficiency	559:580	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	4	60	theme	parameters	772:781	arg1	adsorption					730:739	the adsorption	726:739	the adsorption	726:739	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	4	60	theme	parameters	772:781	arg1	function					746:753	a function	744:753	a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN)	744:914	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	1	61	theme	discharges	141:150	arg1	bodies					161:166	discharges to water bodies	141:166	discharges to water bodies	141:166	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	1	62	theme	inexpensive	231:241	arg1	technologies					243:254	reliable inexpensive technologies	222:254	reliable inexpensive technologies	222:254	As tighter regulations on color in discharges to water bodies are more widely implemented worldwide, the demand for reliable inexpensive technologies for dye removal grows.					
34237369	2	63	theme	operating	441:449	arg1	parameters					451:460	operating parameters	441:460	operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed)	441:554	In this study, the removal of the basic dye, methylene blue, by adsorption onto low-cost sodium alginate-kaolin beads was investigated to determine the effect of operating parameters (initial dye concentration, contact time, pH, adsorbent dosage, temperature, agitation speed) on dye removal efficiency.					
34237369	3	64	theme	analytical	663:672	arg1	techniques					674:683	analytical techniques	663:683	analytical techniques	663:683	The composite beads and individual components were characterized by a number of analytical techniques.					
34237369	4	65	theme	operating	762:770	arg1	parameters					772:781	the operating parameters	758:781	the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN)	758:914	Three models were developed to describe the adsorption as a function of the operating parameters using regression analysis, and two powerful intelligent modeling techniques, genetic programming and artificial neural network (ANN).					
34237369	3	66	theme	techniques	674:683	arg1	number					653:658	a number	651:658	a number of analytical techniques	651:683	The composite beads and individual components were characterized by a number of analytical techniques.					
34237369	8	67	theme	kaolin	1288:1293	arg1	powder					1295:1300	the kaolin powder	1284:1300	the kaolin powder	1284:1300	Encapsulating the kaolin powder in sodium alginate resulted in removal efficiency of 99.56% and a maximum adsorption capacity of 188.7 mg.g-1, a more than fourfold increase over kaolin alone.					
34237369	6	68	from	solutions	1157:1165	arg1	dyes					1139:1142	basic dyes	1133:1142	basic dyes from aqueous solutions	1133:1165	The developed model can be used as a useful tool to optimize treatment processes using the promising adsorbent, to eliminate basic dyes from aqueous solutions.					
32141282	0	0	theme	Antimicrobial	80:92	arg1	Application					94:104	Antimicrobial Application	80:104	Antimicrobial Application	80:104	Polyanionic Composite Membranes Based on Bacterial Cellulose and Amino Acid for Antimicrobial Application.					
32141282	8	1	theme	obtained	1170:1177	arg1	membranes					1179:1187	The obtained membranes	1166:1187	The obtained membranes	1166:1187	The obtained membranes provided a novel promising candidate for wound dressing materials.					
32141282	3	2	theme	BC	478:479	arg1	liquids					501:507	BC and polymeric ionic liquids	478:507	BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity	478:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	7	3	theme	BC/PIL	1148:1153	arg1	membranes					1155:1163	BC/PIL membranes	1148:1163	BC/PIL membranes	1148:1163	The anionic PILs played important roles in the antimicrobial activity of BC/PIL membranes.					
32141282	3	4	dep	liquids	501:507	arg1	BC/PILs					510:516	BC/PILs	510:516	BC/PILs	510:516	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	3	4	dep	liquids	501:507	arg1	PILs					539:542	PILs	539:542	PILs formed by choline and different amino acids	539:586	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	6	5	theme	antimicrobial	991:1003	arg1	activity					1005:1012	good biocompatibility and high antimicrobial activity	960:1012	good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus	960:1072	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	3	6	theme	amino	576:580	arg1	acids					582:586	different amino acids	566:586	different amino acids	566:586	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	7	7	theme	antimicrobial	1122:1134	arg1	activity					1136:1143	the antimicrobial activity	1118:1143	the antimicrobial activity of BC/PIL membranes	1118:1163	The anionic PILs played important roles in the antimicrobial activity of BC/PIL membranes.					
32141282	6	8	theme	high	986:989	arg1	activity					1005:1012	good biocompatibility and high antimicrobial activity	960:1012	good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus	960:1072	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	2	9	theme	dressing	270:277	arg1	materials					279:287	novel wound dressing materials	258:287	novel wound dressing materials	258:287	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	9	theme	dressing	270:277	arg1	deal					237:240	a great deal	229:240	a great deal of attention	229:253	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	4	10	theme	physicochemical	660:674	arg1	properties					694:703	properties	694:703	properties	694:703	The physicochemical and antimicrobial properties and biocompatibility of these membranes were systematically investigated.					
32141282	8	11	theme	novel	1200:1204	arg1	candidate					1216:1224	a novel promising candidate	1198:1224	a novel promising candidate for wound dressing materials	1198:1253	The obtained membranes provided a novel promising candidate for wound dressing materials.					
32141282	1	12	theme	dressing	119:126	arg1	materials					128:136	Ideal wound dressing materials	107:136	Ideal wound dressing materials	107:136	Ideal wound dressing materials should be active components in the healing process.					
32141282	1	13	from	components	155:164	arg1	process					181:187	the healing process	169:187	the healing process	169:187	Ideal wound dressing materials should be active components in the healing process.					
32141282	2	14	theme	wound	264:268	arg1	materials					279:287	novel wound dressing materials	258:287	novel wound dressing materials	258:287	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	14	theme	wound	264:268	arg1	deal					237:240	a great deal	229:240	a great deal of attention	229:253	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	3	15	theme	novel	405:409	arg1	materials					426:434	novel wound dressing materials	405:434	novel wound dressing materials	405:434	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	1	16	theme	healing	173:179	arg1	process					181:187	the healing process	169:187	the healing process	169:187	Ideal wound dressing materials should be active components in the healing process.					
32141282	3	17	with	liquids	501:507	arg1	activity					608:615	antimicrobial activity	594:615	antimicrobial activity	594:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	2	18	theme	novel	258:262	arg1	materials					279:287	novel wound dressing materials	258:287	novel wound dressing materials	258:287	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	18	theme	novel	258:262	arg1	deal					237:240	a great deal	229:240	a great deal of attention	229:253	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	0	19	theme	Composite	12:20	arg1	Membranes					22:30	Polyanionic Composite Membranes	0:30	Polyanionic Composite Membranes	0:30	Polyanionic Composite Membranes Based on Bacterial Cellulose and Amino Acid for Antimicrobial Application.					
32141282	3	20	theme	ionic	495:499	arg1	liquids					501:507	BC and polymeric ionic liquids	478:507	BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity	478:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	7	21	theme	important	1099:1107	arg1	roles					1109:1113	important roles	1099:1113	important roles	1099:1113	The anionic PILs played important roles in the antimicrobial activity of BC/PIL membranes.					
32141282	5	22	dep	concentration	884:896	arg1	the					880:882	the	880:882	the	880:882	The results indicated that BC/PIL membranes with excellent properties could be obtained by adjusting the concentration and type of PILs.					
32141282	3	23	theme	ex	640:641	arg1	method					648:653	an ex situ method	637:653	an ex situ method	637:653	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	0	24	theme	Polyanionic	0:10	arg1	Membranes					22:30	Polyanionic Composite Membranes	0:30	Polyanionic Composite Membranes	0:30	Polyanionic Composite Membranes Based on Bacterial Cellulose and Amino Acid for Antimicrobial Application.					
32141282	6	25	theme	biocompatibility	965:980	arg1	activity					1005:1012	good biocompatibility and high antimicrobial activity	960:1012	good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus	960:1072	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	4	26	theme	antimicrobial	680:692	arg1	properties					694:703	properties	694:703	properties	694:703	The physicochemical and antimicrobial properties and biocompatibility of these membranes were systematically investigated.					
32141282	3	27	theme	wound	411:415	arg1	materials					426:434	novel wound dressing materials	405:434	novel wound dressing materials	405:434	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	8	28	theme	wound	1230:1234	arg1	materials					1245:1253	wound dressing materials	1230:1253	wound dressing materials	1230:1253	The obtained membranes provided a novel promising candidate for wound dressing materials.					
32141282	3	29	dep	ex	640:641	arg1	situ					643:646	situ	643:646	situ	643:646	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	1	30	theme	Ideal	107:111	arg1	materials					128:136	Ideal wound dressing materials	107:136	Ideal wound dressing materials	107:136	Ideal wound dressing materials should be active components in the healing process.					
32141282	3	31	theme	practical	358:366	arg1	values					380:385	the practical application values	354:385	the practical application values of BC	354:391	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	2	32	theme	Bacterial	190:198	arg1	BC					211:212	BC	211:212	BC	211:212	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	32	theme	Bacterial	190:198	arg1	cellulose					200:208	Bacterial cellulose	190:208	Bacterial cellulose (BC)	190:213	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	1	33	theme	wound	113:117	arg1	materials					128:136	Ideal wound dressing materials	107:136	Ideal wound dressing materials	107:136	Ideal wound dressing materials should be active components in the healing process.					
32141282	3	34	theme	polymeric	485:493	arg1	liquids					501:507	BC and polymeric ionic liquids	478:507	BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity	478:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	0	35	theme	Bacterial	41:49	arg1	Cellulose					51:59	Bacterial Cellulose	41:59	Bacterial Cellulose	41:59	Polyanionic Composite Membranes Based on Bacterial Cellulose and Amino Acid for Antimicrobial Application.					
32141282	6	36	theme	membranes	940:948	arg1	kinds					924:928	Several kinds	916:928	Several kinds of BC/PIL membranes	916:948	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	5	37	with	membranes	813:821	arg1	properties					838:847	excellent properties	828:847	excellent properties	828:847	The results indicated that BC/PIL membranes with excellent properties could be obtained by adjusting the concentration and type of PILs.					
32141282	3	38	theme	antimicrobial	594:606	arg1	activity					608:615	antimicrobial activity	594:615	antimicrobial activity	594:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	2	39	theme	great	231:235	arg1	materials					279:287	novel wound dressing materials	258:287	novel wound dressing materials	258:287	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	39	theme	great	231:235	arg1	deal					237:240	a great deal	229:240	a great deal of attention	229:253	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	5	40	theme	excellent	828:836	arg1	properties					838:847	excellent properties	828:847	excellent properties	828:847	The results indicated that BC/PIL membranes with excellent properties could be obtained by adjusting the concentration and type of PILs.					
32141282	3	41	theme	dressing	417:424	arg1	materials					426:434	novel wound dressing materials	405:434	novel wound dressing materials	405:434	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	6	42	theme	BC/PIL	933:938	arg1	membranes					940:948	BC/PIL membranes	933:948	BC/PIL membranes	933:948	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	7	43	theme	anionic	1079:1085	arg1	PILs					1087:1090	The anionic PILs	1075:1090	The anionic PILs	1075:1090	The anionic PILs played important roles in the antimicrobial activity of BC/PIL membranes.					
32141282	8	44	theme	promising	1206:1214	arg1	candidate					1216:1224	a novel promising candidate	1198:1224	a novel promising candidate for wound dressing materials	1198:1253	The obtained membranes provided a novel promising candidate for wound dressing materials.					
32141282	7	45	theme	membranes	1155:1163	arg1	activity					1136:1143	the antimicrobial activity	1118:1143	the antimicrobial activity of BC/PIL membranes	1118:1163	The anionic PILs played important roles in the antimicrobial activity of BC/PIL membranes.					
32141282	8	46	theme	dressing	1236:1243	arg1	materials					1245:1253	wound dressing materials	1230:1253	wound dressing materials	1230:1253	The obtained membranes provided a novel promising candidate for wound dressing materials.					
32141282	6	47	theme	good	960:963	arg1	activity					1005:1012	good biocompatibility and high antimicrobial activity	960:1012	good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus	960:1072	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	2	48	theme	antimicrobial	319:331	arg1	activity					333:340	no intrinsic antimicrobial activity	306:340	no intrinsic antimicrobial activity	306:340	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	3	49	theme	BC	390:391	arg1	values					380:385	the practical application values	354:385	the practical application values of BC	354:391	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	2	50	theme	intrinsic	309:317	arg1	activity					333:340	no intrinsic antimicrobial activity	306:340	no intrinsic antimicrobial activity	306:340	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	6	51	theme	Several	916:922	arg1	kinds					924:928	Several kinds	916:928	Several kinds of BC/PIL membranes	916:948	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	3	52	theme	different	566:574	arg1	acids					582:586	different amino acids	566:586	different amino acids	566:586	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	3	53	theme	composite	449:457	arg1	membranes					459:467	composite membranes	449:467	composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity	449:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	6	54	theme	Gram-negative	1040:1052	arg1	bacteria					1054:1061	Gram-positive and Gram-negative bacteria	1022:1061	Gram-positive and Gram-negative bacteria	1022:1061	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	4	55	theme	membranes	735:743	arg1	biocompatibility					709:724	biocompatibility	709:724	biocompatibility	709:724	The physicochemical and antimicrobial properties and biocompatibility of these membranes were systematically investigated.					
32141282	4	55	theme	membranes	735:743	arg1	properties					694:703	properties	694:703	properties	694:703	The physicochemical and antimicrobial properties and biocompatibility of these membranes were systematically investigated.					
32141282	2	56	theme	attention	245:253	arg1	materials					279:287	novel wound dressing materials	258:287	novel wound dressing materials	258:287	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	56	theme	attention	245:253	arg1	deal					237:240	a great deal	229:240	a great deal of attention	229:253	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	3	57	theme	membranes	459:467	arg1	series					439:444	a series	437:444	a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity	437:615	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
32141282	5	58	theme	PILs	910:913	arg1	concentration					884:896	concentration	884:896	concentration	884:896	The results indicated that BC/PIL membranes with excellent properties could be obtained by adjusting the concentration and type of PILs.					
32141282	5	58	theme	PILs	910:913	arg1	type					902:905	type	902:905	type	902:905	The results indicated that BC/PIL membranes with excellent properties could be obtained by adjusting the concentration and type of PILs.					
32141282	1	59	theme	active	148:153	arg1	components					155:164	active components	148:164	active components	148:164	Ideal wound dressing materials should be active components in the healing process.					
32141282	6	60	theme	Gram-positive	1022:1034	arg1	bacteria					1054:1061	Gram-positive and Gram-negative bacteria	1022:1061	Gram-positive and Gram-negative bacteria	1022:1061	Several kinds of BC/PIL membranes exhibited good biocompatibility and high antimicrobial activity against Gram-positive and Gram-negative bacteria and fungus.					
32141282	0	61	theme	Amino	65:69	arg1	Acid					71:74	Amino Acid	65:74	Amino Acid	65:74	Polyanionic Composite Membranes Based on Bacterial Cellulose and Amino Acid for Antimicrobial Application.					
32141282	2	62	contain	has	302:304	arg2	activity					333:340	no intrinsic antimicrobial activity	306:340	no intrinsic antimicrobial activity	306:340	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	2	62	contain	has	302:304	arg1	it					299:300	it	299:300	it	299:300	Bacterial cellulose (BC) has attracted a great deal of attention as novel wound dressing materials; however, it has no intrinsic antimicrobial activity.					
32141282	5	63	theme	BC/PIL	806:811	arg1	membranes					813:821	BC/PIL membranes	806:821	BC/PIL membranes with excellent properties	806:847	The results indicated that BC/PIL membranes with excellent properties could be obtained by adjusting the concentration and type of PILs.					
32141282	3	64	theme	application	368:378	arg1	values					380:385	the practical application values	354:385	the practical application values of BC	354:391	To explore the practical application values of BC and develop novel wound dressing materials, a series of composite membranes based on BC and polymeric ionic liquids (BC/PILs, composed of BC, and PILs formed by choline and different amino acids) with antimicrobial activity were synthesized by an ex situ method.					
34058958	0	0	theme	microbial	95:103	arg1	colonization					105:116	microbial colonization	95:116	microbial colonization	95:116	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	1	1	theme	diseases	355:362	arg1	risk					209:212	an increased risk	196:212	an increased risk of multiple adverse outcomes in offspring	196:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	1	theme	diseases	355:362	arg1	diseases					355:362	the serious gastrointestinal diseases	326:362	the serious gastrointestinal diseases in neonates	326:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	1	theme	diseases	355:362	arg1	one					319:321	one	319:321	one	319:321	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	6	2	theme	intestinal	1037:1046	arg1	development					1056:1066	intestinal mucosal development	1037:1066	intestinal mucosal development	1037:1066	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	1	3	theme	necrotizing	277:287	arg1	risk					209:212	an increased risk	196:212	an increased risk of multiple adverse outcomes in offspring	196:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	3	theme	necrotizing	277:287	arg1	enterocolitis					289:301	neonatal necrotizing enterocolitis	268:301	neonatal necrotizing enterocolitis (NEC)	268:307	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	3	theme	necrotizing	277:287	arg1	NEC					304:306	NEC	304:306	NEC	304:306	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	8	4	theme	LPS	1220:1222	arg1	exposure					1224:1231	prenatal LPS exposure	1211:1231	prenatal LPS exposure	1211:1231	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	5	from	changes	1258:1264	arg1	microbiome					1284:1293	the intestinal microbiome	1269:1293	the intestinal microbiome of the offspring	1269:1310	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	9	6	theme	LPS-induced	1477:1487	arg1	inflammation					1498:1509	LPS-induced maternal inflammation	1477:1509	LPS-induced maternal inflammation	1477:1509	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	6	7	from	offspring	926:934	arg1	tissue					896:901	Intestinal tissue and feces samples	885:919	tissue	896:901	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	6	7	from	offspring	926:934	arg1	samples					913:919	Intestinal tissue and feces samples	885:919	samples	913:919	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	8	8	theme	intestinal	1273:1282	arg1	microbiome					1284:1293	the intestinal microbiome	1269:1293	the intestinal microbiome of the offspring	1269:1310	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	9	9	theme	intestinal	1619:1628	arg1	microbiome					1630:1639	the intestinal microbiome	1615:1639	the intestinal microbiome	1615:1639	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	6	10	theme	mucosal	1048:1054	arg1	development					1056:1066	intestinal mucosal development	1037:1066	intestinal mucosal development	1037:1066	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	9	11	from	injury	1530:1535	arg1	offspring					1540:1548	offspring	1540:1548	offspring	1540:1548	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	9	12	dep	CONCLUSIONS	1433:1443	arg1	suggest					1464:1470	suggest	1464:1470	suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome	1464:1639	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	9	13	theme	microbiome	1630:1639	arg1	composition					1600:1610	the composition	1596:1610	the composition of the intestinal microbiome	1596:1639	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	7	14	dep	RESULTS	1069:1075	arg1	observed					1161:1168	observed	1161:1168	was observed on day 0 and 3 after birth	1157:1195	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	4	15	theme	intraperitoneal	654:668	arg1	injections					670:679	intraperitoneal injections	654:679	intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline	654:772	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	0	16	theme	neonatal	125:132	arg1	period					134:139	the neonatal period	121:139	the neonatal period	121:139	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	3	17	theme	intestinal	580:589	arg1	microbiome					591:600	the intestinal microbiome	576:600	the intestinal microbiome	576:600	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	4	18	theme	bacterial	743:751	arg1	endotoxin					753:761	bacterial endotoxin	743:761	bacterial endotoxin	743:761	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	3	19	theme	maternal	478:485	arg1	inflammation					487:498	bacterial endotoxin-induced maternal inflammation	450:498	bacterial endotoxin-induced maternal inflammation	450:498	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	0	20	theme	fetal	59:63	arg1	injury					76:81	fetal intestinal injury	59:81	fetal intestinal injury	59:81	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	9	21	from	changes	1585:1591	arg1	composition					1600:1610	the composition	1596:1610	the composition of the intestinal microbiome	1596:1639	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	3	22	theme	microbiome	591:600	arg1	composition					561:571	the composition	557:571	the composition of the intestinal microbiome	557:600	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	1	23	theme	increased	199:207	arg1	risk					209:212	an increased risk	196:212	an increased risk of multiple adverse outcomes in offspring	196:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	23	theme	increased	199:207	arg1	enterocolitis					289:301	neonatal necrotizing enterocolitis	268:301	neonatal necrotizing enterocolitis (NEC)	268:307	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	23	theme	increased	199:207	arg1	diseases					355:362	the serious gastrointestinal diseases	326:362	the serious gastrointestinal diseases in neonates	326:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	23	theme	increased	199:207	arg1	one					319:321	one	319:321	one	319:321	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	0	24	theme	endotoxin-induced	10:26	arg1	inflammation					37:48	Bacterial endotoxin-induced maternal inflammation	0:48	Bacterial endotoxin-induced maternal inflammation	0:48	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	6	25	theme	intestinal	1003:1012	arg1	colonization					1020:1031	intestinal flora colonization	1003:1031	intestinal flora colonization	1003:1031	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	8	26	from	decrease	1387:1394	arg1	7 days					1413:1418	7 days	1413:1418	7 days after birth	1413:1430	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	26	from	decrease	1387:1394	arg1	Escherichia-Shigella					1359:1378	Escherichia-Shigella	1359:1378	Escherichia-Shigella	1359:1378	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	26	from	decrease	1387:1394	arg1	Proteobacteria					1343:1356	Proteobacteria	1343:1356	Proteobacteria (Escherichia-Shigella)	1343:1379	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	26	from	decrease	1387:1394	arg1	Firmicutes					1399:1408	Firmicutes	1399:1408	Firmicutes	1399:1408	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	27	theme	offspring	1302:1310	arg1	microbiome					1284:1293	the intestinal microbiome	1269:1293	the intestinal microbiome of the offspring	1269:1310	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	3	28	theme	bacterial	450:458	arg1	inflammation					487:498	bacterial endotoxin-induced maternal inflammation	450:498	bacterial endotoxin-induced maternal inflammation	450:498	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	0	29	theme	Bacterial	0:8	arg1	inflammation					37:48	Bacterial endotoxin-induced maternal inflammation	0:48	Bacterial endotoxin-induced maternal inflammation	0:48	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	2	30	theme	underlying	390:399	arg1	mechanism					401:409	the underlying mechanism	386:409	the underlying mechanism	386:409	However, the underlying mechanism remains undefined.					
34058958	4	31	theme	Pregnant	611:618	arg1	rats					635:638	Pregnant Sprague Dawley rats	611:638	Pregnant Sprague Dawley rats	611:638	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	7	32	theme	LPS	1144:1146	arg1	exposure					1148:1155	prenatal LPS exposure	1135:1155	prenatal LPS exposure	1135:1155	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	1	33	theme	multiple	217:224	arg1	outcomes					234:241	multiple adverse outcomes	217:241	multiple adverse outcomes in offspring	217:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	0	34	theme	maternal	28:35	arg1	inflammation					37:48	Bacterial endotoxin-induced maternal inflammation	0:48	Bacterial endotoxin-induced maternal inflammation	0:48	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	6	35	theme	flora	1014:1018	arg1	colonization					1020:1031	intestinal flora colonization	1003:1031	intestinal flora colonization	1003:1031	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	8	36	from	increase	1331:1338	arg1	7 days					1413:1418	7 days	1413:1418	7 days after birth	1413:1430	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	36	from	increase	1331:1338	arg1	Escherichia-Shigella					1359:1378	Escherichia-Shigella	1359:1378	Escherichia-Shigella	1359:1378	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	36	from	increase	1331:1338	arg1	Proteobacteria					1343:1356	Proteobacteria	1343:1356	Proteobacteria (Escherichia-Shigella)	1343:1379	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	36	from	increase	1331:1338	arg1	Firmicutes					1399:1408	Firmicutes	1399:1408	Firmicutes	1399:1408	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	37	theme	prenatal	1211:1218	arg1	exposure					1224:1231	prenatal LPS exposure	1211:1231	prenatal LPS exposure	1211:1231	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	1	38	theme	adverse	226:232	arg1	outcomes					234:241	multiple adverse outcomes	217:241	multiple adverse outcomes in offspring	217:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	4	39	theme	Dawley	628:633	arg1	rats					635:638	Pregnant Sprague Dawley rats	611:638	Pregnant Sprague Dawley rats	611:638	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	6	40	theme	feces	907:911	arg1	samples					913:919	Intestinal tissue and feces samples	885:919	samples	913:919	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	3	41	theme	endotoxin-induced	460:476	arg1	inflammation					487:498	bacterial endotoxin-induced maternal inflammation	450:498	bacterial endotoxin-induced maternal inflammation	450:498	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	1	42	theme	outcomes	234:241	arg1	risk					209:212	an increased risk	196:212	an increased risk of multiple adverse outcomes in offspring	196:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	42	theme	outcomes	234:241	arg1	enterocolitis					289:301	neonatal necrotizing enterocolitis	268:301	neonatal necrotizing enterocolitis (NEC)	268:307	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	42	theme	outcomes	234:241	arg1	diseases					355:362	the serious gastrointestinal diseases	326:362	the serious gastrointestinal diseases in neonates	326:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	42	theme	outcomes	234:241	arg1	one					319:321	one	319:321	one	319:321	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	7	43	theme	prenatal	1135:1142	arg1	exposure					1148:1155	prenatal LPS exposure	1135:1155	prenatal LPS exposure	1135:1155	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	1	44	from	outcomes	234:241	arg1	offspring					246:254	offspring	246:254	offspring	246:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	3	45	theme	intestinal	507:516	arg1	injury					518:523	intestinal injury	507:523	intestinal injury in offspring	507:536	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	0	46	theme	intestinal	65:74	arg1	injury					76:81	fetal intestinal injury	59:81	fetal intestinal injury	59:81	Bacterial endotoxin-induced maternal inflammation leads to fetal intestinal injury and affects microbial colonization in the neonatal period.					
34058958	6	47	theme	inflammation	987:998	arg1	effects					967:973	the effects	963:973	the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development	963:1066	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	7	48	theme	offspring	1114:1122	arg1	injury					1100:1105	Significant intestinal injury	1077:1105	Significant intestinal injury of the offspring induced by prenatal LPS exposure	1077:1155	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	1	49	from	diseases	355:362	arg1	neonates					367:374	neonates	367:374	neonates	367:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	6	50	theme	maternal	978:985	arg1	inflammation					987:998	maternal inflammation	978:998	maternal inflammation	978:998	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	4	51	dep	METHODS	603:609	arg1	received					645:652	received	645:652	were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation	640:796	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	6	52	theme	Intestinal	885:894	arg1	tissue					896:901	Intestinal tissue and feces samples	885:919	tissue	896:901	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	3	53	from	injury	518:523	arg1	offspring					528:536	offspring	528:536	offspring	528:536	We hypothesize that bacterial endotoxin-induced maternal inflammation causes intestinal injury in offspring, thereby affecting the composition of the intestinal microbiome.					
34058958	9	54	theme	NEC-like	1576:1583	arg1	changes					1585:1591	NEC-like changes	1576:1591	NEC-like changes in the composition of the intestinal microbiome	1576:1639	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	1	55	theme	serious	330:336	arg1	diseases					355:362	the serious gastrointestinal diseases	326:362	the serious gastrointestinal diseases in neonates	326:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	56	from	offspring	246:254	arg1	risk					209:212	an increased risk	196:212	an increased risk of multiple adverse outcomes in offspring	196:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	56	from	offspring	246:254	arg1	enterocolitis					289:301	neonatal necrotizing enterocolitis	268:301	neonatal necrotizing enterocolitis (NEC)	268:307	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	56	from	offspring	246:254	arg1	diseases					355:362	the serious gastrointestinal diseases	326:362	the serious gastrointestinal diseases in neonates	326:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	56	from	offspring	246:254	arg1	one					319:321	one	319:321	one	319:321	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	4	57	dep	lipopolysaccharide	696:713	arg1	same					735:738	same	735:738	same	735:738	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	4	57	dep	lipopolysaccharide	696:713	arg1	LPS					716:718	LPS	716:718	LPS	716:718	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	4	58	with	injections	670:679	arg1	saline					767:772	saline	767:772	saline	767:772	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	4	58	with	injections	670:679	arg1	lipopolysaccharide					696:713	700 μg/kg lipopolysaccharide	686:713	700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin)	686:762	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	7	59	theme	Significant	1077:1087	arg1	injury					1100:1105	Significant intestinal injury	1077:1105	Significant intestinal injury of the offspring induced by prenatal LPS exposure	1077:1155	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	1	60	theme	BACKGROUND	142:151	arg1	infection					167:175	BACKGROUND Intraamniotic infection	142:175	BACKGROUND Intraamniotic infection	142:175	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	8	61	from	7 days	1413:1418	arg1	decrease					1387:1394	a decrease	1385:1394	a decrease in Firmicutes at 7 days after birth	1385:1430	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	8	61	from	7 days	1413:1418	arg1	increase					1331:1338	a significant increase	1317:1338	a significant increase in Proteobacteria (Escherichia-Shigella)	1317:1379	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	4	62	theme	700 μg/kg	686:694	arg1	lipopolysaccharide					696:713	700 μg/kg lipopolysaccharide	686:713	700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin)	686:762	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	7	63	theme	intestinal	1089:1098	arg1	injury					1100:1105	Significant intestinal injury	1077:1105	Significant intestinal injury of the offspring induced by prenatal LPS exposure	1077:1155	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	1	64	from	risk	209:212	arg1	offspring					246:254	offspring	246:254	offspring	246:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	65	theme	Intraamniotic	153:165	arg1	infection					167:175	BACKGROUND Intraamniotic infection	142:175	BACKGROUND Intraamniotic infection	142:175	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	7	66	located	observed	1161:1168	arg2	injury					1100:1105	Significant intestinal injury	1077:1105	Significant intestinal injury of the offspring induced by prenatal LPS exposure	1077:1155	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	7	66	located	observed	1161:1168	arg1	day					1173:1175	day 0 and 3	1173:1183	day	1173:1175	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	7	66	located	observed	1161:1168	arg1	3					1183:1183	3	1183:1183	3	1183:1183	RESULTS Significant intestinal injury of the offspring induced by prenatal LPS exposure was observed on day 0 and 3 after birth.					
34058958	4	67	theme	gestation	788:796	arg1	15 days					777:783	15 days	777:783	15 days of gestation	777:796	METHODS Pregnant Sprague Dawley rats were received intraperitoneal injections with 700 μg/kg lipopolysaccharide (LPS, which was the same as bacterial endotoxin) or saline at 15 days of gestation.					
34058958	8	68	theme	significant	1319:1329	arg1	increase					1331:1338	a significant increase	1317:1338	a significant increase in Proteobacteria (Escherichia-Shigella)	1317:1379	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	9	69	theme	intestinal	1519:1528	arg1	injury					1530:1535	intestinal injury	1519:1535	intestinal injury in offspring	1519:1548	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	9	70	theme	maternal	1489:1496	arg1	inflammation					1498:1509	LPS-induced maternal inflammation	1477:1509	LPS-induced maternal inflammation	1477:1509	CONCLUSIONS Thus, our findings suggest that LPS-induced maternal inflammation induces intestinal injury in offspring and subsequently leads to NEC-like changes in the composition of the intestinal microbiome.					
34058958	1	71	theme	gastrointestinal	338:353	arg1	diseases					355:362	the serious gastrointestinal diseases	326:362	the serious gastrointestinal diseases in neonates	326:374	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	8	72	theme	significant	1246:1256	arg1	changes					1258:1264	significant changes	1246:1264	significant changes in the intestinal microbiome of the offspring	1246:1310	In addition, prenatal LPS exposure also induced significant changes in the intestinal microbiome of the offspring with a significant increase in Proteobacteria (Escherichia-Shigella) and a decrease in Firmicutes at 7 days after birth.					
34058958	6	73	from	effects	967:973	arg1	development					1056:1066	intestinal mucosal development	1037:1066	intestinal mucosal development	1037:1066	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	6	73	from	effects	967:973	arg1	colonization					1020:1031	intestinal flora colonization	1003:1031	intestinal flora colonization	1003:1031	Intestinal tissue and feces samples from offspring were collected to evaluate the effects of maternal inflammation on intestinal flora colonization and intestinal mucosal development.					
34058958	1	74	theme	neonatal	268:275	arg1	risk					209:212	an increased risk	196:212	an increased risk of multiple adverse outcomes in offspring	196:254	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	74	theme	neonatal	268:275	arg1	enterocolitis					289:301	neonatal necrotizing enterocolitis	268:301	neonatal necrotizing enterocolitis (NEC)	268:307	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34058958	1	74	theme	neonatal	268:275	arg1	NEC					304:306	NEC	304:306	NEC	304:306	BACKGROUND Intraamniotic infection is associated with an increased risk of multiple adverse outcomes in offspring, especially neonatal necrotizing enterocolitis (NEC), which is one of the serious gastrointestinal diseases in neonates.					
34724615	0	0	theme	Oxidized	82:89	arg1	Nanocellulose					101:113	Oxidized Bacterial Nanocellulose	82:113	Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration	82:148	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	1	1	theme	donor	304:308	arg1	nerve					310:314	a donor nerve and secondary injuries	302:337	nerve	310:314	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	1	2	from	standard	183:190	arg1	treatment					208:216	the clinical treatment	195:216	the clinical treatment	195:216	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	2	3	dep	the	468:470	arg1	substitutes					472:482	substitutes	472:482	substitutes	472:482	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	1	4	theme	nerve	310:314	arg1	availability					286:297	the availability	282:297	the availability of a donor nerve and secondary injuries	282:337	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	4	5	theme	antibacterial	830:842	arg1	activity					844:851	antibacterial activity	830:851	antibacterial activity	830:851	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	3	6	theme	bacterial	631:639	arg1	cellulose					641:649	bacterial cellulose	631:649	situ in an oxidized bacterial cellulose (OBC) conduit	611:663	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	6	theme	bacterial	631:639	arg1	OBC					652:654	OBC	652:654	OBC	652:654	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	4	7	theme	NGF	797:799	arg1	nanocomposite					811:823	A novel NGF@CSNPs/OBC nanocomposite	789:823	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure	789:896	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	0	8	theme	Bacterial	91:99	arg1	Nanocellulose					101:113	Oxidized Bacterial Nanocellulose	82:113	Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration	82:148	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	1	9	theme	secondary	320:328	arg1	injuries					330:337	a donor nerve and secondary injuries	302:337	injuries	330:337	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	4	10	theme	novel	791:795	arg1	nanocomposite					811:823	A novel NGF@CSNPs/OBC nanocomposite	789:823	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure	789:896	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	6	11	theme	rats	1115:1118	arg1	defect					1105:1110	the sciatic nerve defect	1087:1110	the sciatic nerve defect of rats	1087:1118	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	5	12	dep	adhesion	990:997	arg1	the					986:988	the	986:988	the	986:988	In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells.					
34724615	0	13	theme	Rat	119:121	arg1	Regeneration					137:148	Rat Sciatic Nerve Regeneration	119:148	Rat Sciatic Nerve Regeneration	119:148	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	1	14	theme	injuries	330:337	arg1	availability					286:297	the availability	282:297	the availability of a donor nerve and secondary injuries	282:337	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	7	15	theme	regenerated	1274:1284	arg1	nerve					1286:1290	the regenerated nerve	1270:1290	the regenerated nerve	1270:1290	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	4	16	theme	porous	876:881	arg1	microstructure					883:896	porous microstructure	876:896	porous microstructure	876:896	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	2	17	theme	nerves	445:450	arg1	regeneration					421:432	the regeneration	417:432	the regeneration of injured nerves	417:450	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	1	18	theme	standard	183:190	arg1	gold					178:181	the gold	174:181	the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries	174:337	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	1	18	theme	standard	183:190	arg1	Autograft					151:159	Autograft	151:159	Autograft	151:159	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	6	19	theme	nerve	1099:1103	arg1	defect					1105:1110	the sciatic nerve defect	1087:1110	the sciatic nerve defect of rats	1087:1118	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	8	20	theme	novel	1459:1463	arg1	NGC					1465:1467	a novel NGC	1457:1467	a novel NGC	1457:1467	In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.					
34724615	3	21	theme	growth	521:526	arg1	NGF					536:538	NGF	536:538	NGF	536:538	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	21	theme	growth	521:526	arg1	factor					528:533	nerve growth factor	515:533	nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs)	515:583	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	8	22	theme	great	1478:1482	arg1	potential					1484:1492	great potential	1478:1492	great potential	1478:1492	In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.					
34724615	0	23	theme	In	0:1	arg1	Fabrication					8:18	In Situ Fabrication	0:18	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan	0:63	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	3	24	theme	gel	679:681	arg1	method					683:688	the ion gel method	671:688	the ion gel method	671:688	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	6	25	theme	defect	1160:1165	arg1	repair					1134:1139	a successful repair	1121:1139	a successful repair of the 10 mm nerve defect	1121:1165	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	3	26	theme	factor	528:533	arg1	nanoparticles					563:575	nerve growth factor (NGF) encapsulated chitosan nanoparticles	515:575	nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs)	515:583	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	26	theme	factor	528:533	arg1	CSNPs					578:582	CSNPs	578:582	CSNPs	578:582	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	2	27	theme	guidance	346:353	arg1	NGC					365:367	NGC	365:367	NGC	365:367	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	2	27	theme	guidance	346:353	arg1	conduits					355:362	Nerve guidance conduits	340:362	Nerve guidance conduits (NGC)	340:368	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	6	28	theme	successful	1123:1132	arg1	repair					1134:1139	a successful repair	1121:1139	a successful repair of the 10 mm nerve defect	1121:1165	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	0	29	theme	Nerve	131:135	arg1	Regeneration					137:148	Rat Sciatic Nerve Regeneration	119:148	Rat Sciatic Nerve Regeneration	119:148	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	7	30	theme	nerve	1286:1290	arg1	histology					1232:1240	histology	1232:1240	histology	1232:1240	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	7	30	theme	nerve	1286:1290	arg1	morphology					1220:1229	morphology	1220:1229	morphology	1220:1229	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	7	30	theme	nerve	1286:1290	arg1	recovery					1258:1265	functional recovery	1247:1265	functional recovery	1247:1265	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	7	30	theme	nerve	1286:1290	arg1	diameter					1210:1217	diameter	1210:1217	diameter	1210:1217	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	4	31	with	nanocomposite	811:823	arg1	activity					844:851	antibacterial activity	830:851	antibacterial activity	830:851	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	4	31	with	nanocomposite	811:823	arg1	microstructure					883:896	porous microstructure	876:896	porous microstructure	876:896	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	4	31	with	nanocomposite	811:823	arg1	biodegradability					854:869	biodegradability	854:869	biodegradability	854:869	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	1	32	theme	clinical	199:206	arg1	treatment					208:216	the clinical treatment	195:216	the clinical treatment	195:216	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	6	33	theme	sciatic	1091:1097	arg1	defect					1105:1110	the sciatic nerve defect	1087:1110	the sciatic nerve defect of rats	1087:1118	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	7	34	dep	regeneration	1375:1386	arg1	the					1371:1373	the	1371:1373	the	1371:1373	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	0	35	theme	Sciatic	123:129	arg1	Regeneration					137:148	Rat Sciatic Nerve Regeneration	119:148	Rat Sciatic Nerve Regeneration	119:148	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	3	36	theme	nerve	515:519	arg1	NGF					536:538	NGF	536:538	NGF	536:538	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	36	theme	nerve	515:519	arg1	factor					528:533	nerve growth factor	515:533	nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs)	515:583	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	8	37	theme	@	1443:1443	arg1	CSNPs/OBC					1444:1452	the NGF@CSNPs/OBC	1436:1452	the NGF@CSNPs/OBC as a novel NGC	1436:1467	In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.					
34724615	0	38	theme	Growth	29:34	arg1	Factor					36:41	Nerve Growth Factor	23:41	Nerve Growth Factor Encapsulated Chitosan	23:63	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	3	39	theme	ion	675:677	arg1	method					683:688	the ion gel method	671:688	the ion gel method	671:688	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	6	40	theme	mm	1151:1152	arg1	defect					1160:1165	the 10 mm nerve defect	1144:1165	the 10 mm nerve defect	1144:1165	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	5	41	theme	Schwann	1020:1026	arg1	cells					1028:1032	Schwann cells	1020:1032	Schwann cells	1020:1032	In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells.					
34724615	6	42	theme	nerve	1154:1158	arg1	defect					1160:1165	the 10 mm nerve defect	1144:1165	the 10 mm nerve defect	1144:1165	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	5	43	dep	In	926:927	arg1	vitro					929:933	vitro	929:933	vitro	929:933	In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells.					
34724615	0	44	theme	Nerve	23:27	arg1	Factor					36:41	Nerve Growth Factor	23:41	Nerve Growth Factor Encapsulated Chitosan	23:63	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	2	45	theme	injured	437:443	arg1	nerves					445:450	injured nerves	437:450	injured nerves	437:450	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	5	46	theme	cells	1028:1032	arg1	proliferation					1003:1015	proliferation	1003:1015	proliferation	1003:1015	In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells.					
34724615	5	46	theme	cells	1028:1032	arg1	adhesion					990:997	adhesion	990:997	adhesion	990:997	In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells.					
34724615	3	47	theme	encapsulated	541:552	arg1	nanoparticles					563:575	nerve growth factor (NGF) encapsulated chitosan nanoparticles	515:575	nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs)	515:583	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	47	theme	encapsulated	541:552	arg1	CSNPs					578:582	CSNPs	578:582	CSNPs	578:582	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	7	48	from	week	1198:1201	arg1	comparable					1296:1305	comparable	1296:1305	comparable	1296:1305	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	1	49	theme	peripheral	221:230	arg1	PNI					246:248	PNI	246:248	PNI	246:248	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	1	49	theme	peripheral	221:230	arg1	injury					238:243	peripheral nerve injury	221:243	peripheral nerve injury (PNI)	221:249	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	0	50	theme	Encapsulated	43:54	arg1	Chitosan					56:63	Nerve Growth Factor Encapsulated Chitosan	23:63	Nerve Growth Factor Encapsulated Chitosan	23:63	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	4	51	theme	CSNPs/OBC	801:809	arg1	nanocomposite					811:823	A novel NGF@CSNPs/OBC nanocomposite	789:823	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure	789:896	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	6	52	theme	10	1148:1149	arg1	mm					1151:1152	mm	1151:1152	mm	1151:1152	When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks.					
34724615	0	53	theme	Factor	36:41	arg1	Chitosan					56:63	Nerve Growth Factor Encapsulated Chitosan	23:63	Nerve Growth Factor Encapsulated Chitosan	23:63	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	3	54	dep	conduit	657:663	arg1	in					616:617	situ in an oxidized bacterial cellulose (OBC) conduit	611:663	situ in an oxidized bacterial cellulose (OBC) conduit	611:663	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	4	55	theme	@	800:800	arg1	nanocomposite					811:823	A novel NGF@CSNPs/OBC nanocomposite	789:823	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure	789:896	A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed.					
34724615	8	56	theme	NGF	1440:1442	arg1	CSNPs/OBC					1444:1452	the NGF@CSNPs/OBC	1436:1452	the NGF@CSNPs/OBC as a novel NGC	1436:1467	In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.					
34724615	2	57	dep	microenvironment	389:404	arg1	promote					409:415	promote	409:415	to promote the regeneration of injured nerves	406:450	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	7	58	dep	diameter	1210:1217	arg1	the					1206:1208	the	1206:1208	the	1206:1208	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	7	59	theme	nerve	1417:1421	arg1	regeneration					1375:1386	regeneration	1375:1386	regeneration	1375:1386	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	7	59	theme	nerve	1417:1421	arg1	recovery					1401:1408	function recovery	1392:1408	function recovery	1392:1408	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	3	60	theme	oxidized	622:629	arg1	conduit					657:663	an oxidized bacterial cellulose (OBC) conduit	619:663	situ in an oxidized bacterial cellulose (OBC) conduit	611:663	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	5	61	theme	In	926:927	arg1	experiments					935:945	In vitro experiments	926:945	In vitro experiments	926:945	In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells.					
34724615	1	62	from	treatment	208:216	arg1	standard					183:190	standard	183:190	standard	183:190	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	3	63	theme	NGF	784:786	arg1	release					773:779	a sustainable release	759:779	a sustainable release of NGF	759:786	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	1	64	theme	nerve	232:236	arg1	PNI					246:248	PNI	246:248	PNI	246:248	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	1	64	theme	nerve	232:236	arg1	injury					238:243	peripheral nerve injury	221:243	peripheral nerve injury (PNI)	221:249	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	7	65	theme	function	1392:1399	arg1	recovery					1401:1408	function recovery	1392:1408	function recovery	1392:1408	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	7	66	theme	functional	1247:1256	arg1	recovery					1258:1265	functional recovery	1247:1265	functional recovery	1247:1265	At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve.					
34724615	3	67	theme	sustainable	761:771	arg1	release					773:779	a sustainable release	759:779	a sustainable release of NGF	759:786	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	1	68	theme	injury	238:243	arg1	treatment					208:216	the clinical treatment	195:216	the clinical treatment	195:216	Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries.					
34724615	3	69	theme	situ	611:614	arg1	conduit					657:663	an oxidized bacterial cellulose (OBC) conduit	619:663	situ in an oxidized bacterial cellulose (OBC) conduit	611:663	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	70	theme	chitosan	554:561	arg1	nanoparticles					563:575	nerve growth factor (NGF) encapsulated chitosan nanoparticles	515:575	nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs)	515:583	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	70	theme	chitosan	554:561	arg1	CSNPs					578:582	CSNPs	578:582	CSNPs	578:582	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	2	71	theme	suitable	380:387	arg1	microenvironment					389:404	a suitable microenvironment	378:404	a suitable microenvironment	378:404	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	2	71	theme	suitable	380:387	arg1	the					468:470	the	468:470	the	468:470	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	2	72	theme	Nerve	340:344	arg1	NGC					365:367	NGC	365:367	NGC	365:367	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	2	72	theme	Nerve	340:344	arg1	conduits					355:362	Nerve guidance conduits	340:362	Nerve guidance conduits (NGC)	340:368	Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts.					
34724615	8	73	theme	PNI	1514:1516	arg1	treatment					1501:1509	the treatment	1497:1509	the treatment of PNI	1497:1516	In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.					
34724615	0	74	theme	Chitosan	56:63	arg1	Fabrication					8:18	In Situ Fabrication	0:18	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan	0:63	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	3	75	theme	cellulose	641:649	arg1	conduit					657:663	an oxidized bacterial cellulose (OBC) conduit	619:663	situ in an oxidized bacterial cellulose (OBC) conduit	611:663	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	0	76	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration.					
34724615	3	77	theme	solution	725:732	arg1	introduction					700:711	the introduction	696:711	the introduction of a CS/NGF solution	696:732	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
34724615	3	78	theme	CS/NGF	718:723	arg1	solution					725:732	a CS/NGF solution	716:732	a CS/NGF solution	716:732	In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF.					
33560509	10	0	theme	treated	1660:1666	arg1	P					1676:1676	P ˂ 0.05	1676:1683	P ˂ 0.05	1676:1683	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	10	0	theme	treated	1660:1666	arg1	groups					1668:1673	all treated groups	1656:1673	all treated groups (P ˂ 0.05)	1656:1684	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	0	1	theme	Nile	172:175	arg1	tilapia					177:183	Nile tilapia	172:183	Nile tilapia	172:183	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	13	2	theme	fish	2033:2036	arg1	groups					2038:2043	fish groups	2033:2043	fish groups fed diet with a combination of ZEO and ChNPs	2033:2088	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	4	3	theme	growth	808:813	arg1	P					827:827	P ˂ 0.05	827:834	P ˂ 0.05	827:834	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	4	3	theme	growth	808:813	arg1	parameters					815:824	all growth parameters	804:824	all growth parameters (P ˂ 0.05)	804:835	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	14	4	located	observed	2162:2169	arg1	treatment					2199:2207	fish group with combined treatment	2174:2207	fish group with combined treatment with ChNPs and ZEO	2174:2226	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	14	4	located	observed	2162:2169	arg2	protection of					2111:2123	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	3	5	dep	IMID	728:731	arg1	LC50					744:747	½ of 96 h LC50	734:747	½ of 96 h LC50 = 0.0545 μg L-1	734:763	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	1	6	theme	effects	263:269	arg1	evaluation					234:243	the evaluation	230:243	the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	230:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	2	7	theme	other	517:521	arg1	groups					523:528	the other groups	513:528	the other groups	513:528	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	8	theme	g	602:602	arg1	ZEO					587:589	ZEO	587:589	ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40)	587:626	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	8	theme	g	602:602	arg1	kg-1					604:607	20 and 40 g kg-1	592:607	20 and 40 g kg-1	592:607	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	12	9	from	fish	1850:1853	arg1	group					1866:1870	the CTR group	1858:1870	the CTR group	1858:1870	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	13	10	theme	ChNPs	2084:2088	arg1	combination					2061:2071	a combination	2059:2071	a combination of ZEO and ChNPs	2059:2088	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	15	11	theme	Nile	2283:2286	arg1	diets					2296:2300	Nile tilapia diets	2283:2300	Nile tilapia diets	2283:2300	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	15	12	theme	toxic	2349:2353	arg1	impacts					2355:2361	the toxic impacts	2345:2361	the toxic impacts of IMID	2345:2369	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	4	13	theme	significant	857:867	arg1	effects					869:875	no significant effects	854:875	no significant effects	854:875	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	15	14	theme	tilapia	2288:2294	arg1	diets					2296:2300	Nile tilapia diets	2283:2300	Nile tilapia diets	2283:2300	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	14	15	theme	splenic	2141:2147	arg1	tissues					2149:2155	the hepatic and splenic tissues	2125:2155	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	6	16	theme	ZEO	1020:1022	arg1	impacts					1002:1008	No significant impacts	987:1008	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05)	987:1056	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	8	17	theme	Fish	1338:1341	arg1	diets					1347:1351	Fish fed diets	1338:1351	Fish fed diets with ChNPs and/or ZEO	1338:1373	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	14	18	theme	hepatic	2129:2135	arg1	tissues					2149:2155	the hepatic and splenic tissues	2125:2155	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	6	19	theme	ChNPs	1013:1017	arg1	impacts					1002:1008	No significant impacts	987:1008	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05)	987:1056	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	2	20	theme	g	572:572	arg1	kg-1					574:577	5 g kg-1	570:577	5 g kg-1	570:577	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	20	theme	g	572:572	arg1	ChNPs					563:567	ChNPs	563:567	ChNPs (5 g kg-1)	563:578	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	1	21	from	evaluation	234:243	arg1	toxicity					364:371	imidacloprid (IMID)-induced toxicity	336:371	imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	336:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	22	theme	Nile	376:379	arg1	tilapia					381:387	Nile tilapia	376:387	Nile tilapia (Oreochromis niloticus)	376:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	22	theme	Nile	376:379	arg1	niloticus					402:410	Oreochromis niloticus	390:410	Oreochromis niloticus	390:410	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	9	23	theme	glucose	1526:1532	arg1	levels					1559:1564	the lowest glucose and alkaline phosphatase levels	1515:1564	the lowest glucose and alkaline phosphatase levels	1515:1564	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	2	24	theme	5	570:570	arg1	g					572:572	g	572:572	g	572:572	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	12	25	dep	malondialdehyde	1888:1902	arg1	levels					1928:1933	levels	1928:1933	levels	1928:1933	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	12	25	dep	malondialdehyde	1888:1902	arg1	the					1876:1878	the	1876:1878	the	1876:1878	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	7	26	theme	combined	1317:1324	arg1	treatments					1326:1335	all combined treatments	1313:1335	all combined treatments	1313:1335	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	7	27	theme	ZEO20	1212:1216	arg1	groups					1218:1223	ChNP and ZEO20 groups	1203:1223	ChNP and ZEO20 groups	1203:1223	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	12	28	theme	other	1838:1842	arg1	hand					1844:1847	the other hand	1834:1847	the other hand	1834:1847	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	9	29	theme	alkaline	1538:1545	arg1	phosphatase					1547:1557	alkaline phosphatase	1538:1557	alkaline phosphatase	1538:1557	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	1	30	theme	nanoparticle	313:324	arg1	effects					263:269	the mitigating effects	248:269	the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	248:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	8	31	theme	significant	1379:1389	arg1	increments					1391:1400	significant increments	1379:1400	significant increments in the MCV values (P ˂ 0.05)	1379:1429	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	7	32	theme	ChNP	1203:1206	arg1	groups					1218:1223	ChNP and ZEO20 groups	1203:1223	ChNP and ZEO20 groups	1203:1223	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	1	33	theme	zeolites	282:289	arg1	effects					263:269	the mitigating effects	248:269	the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	248:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	10	34	theme	transferase	1605:1615	arg1	levels					1617:1622	Serum aspartate transferase levels	1589:1622	Serum aspartate transferase levels	1589:1622	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	6	35	theme	P	1048:1048	arg1	interaction					1035:1045	their interaction	1029:1045	their interaction (P > 0.05)	1029:1056	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	6	35	theme	P	1048:1048	arg1	>					1050:1050	P > 0.05	1048:1055	P > 0.05	1048:1055	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	7	36	theme	ZEO40	1297:1301	arg1	group					1303:1307	the ZEO40 group	1293:1307	the ZEO40 group	1293:1307	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	11	37	theme	nitro	1789:1793	arg1	tetrazolium					1800:1810	nitro blue tetrazolium	1789:1810	nitro blue tetrazolium	1789:1810	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	10	38	theme	Serum	1589:1593	arg1	levels					1617:1622	Serum aspartate transferase levels	1589:1622	Serum aspartate transferase levels	1589:1622	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	6	39	theme	interaction	1035:1045	arg1	impacts					1002:1008	No significant impacts	987:1008	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05)	987:1056	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	8	40	theme	MCV	1409:1411	arg1	P					1421:1421	P ˂ 0.05	1421:1428	P ˂ 0.05	1421:1428	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	8	40	theme	MCV	1409:1411	arg1	values					1413:1418	the MCV values	1405:1418	the MCV values (P ˂ 0.05)	1405:1429	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	2	41	dep	ZEO	587:589	arg1	ZEO40					621:625	ZEO40	621:625	ZEO40	621:625	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	41	dep	ZEO	587:589	arg1	ZEO20					611:615	ZEO20	611:615	ZEO20	611:615	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	7	42	theme	˂	1192:1192	arg1	P					1190:1190	P ˂ 0.05	1190:1197	P ˂ 0.05	1190:1197	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	11	43	theme	lysozyme	1776:1783	arg1	P					1820:1820	P ˂ 0.05	1820:1827	P ˂ 0.05	1820:1827	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	11	43	theme	lysozyme	1776:1783	arg1	values					1812:1817	the highest lysozyme and nitro blue tetrazolium values	1764:1817	the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05)	1764:1828	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	14	44	dep	protection of	2111:2123	arg1	tissues					2149:2155	the hepatic and splenic tissues	2125:2155	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	3	45	theme	last	648:651	arg1	days					656:659	the last 14 days	644:659	the last 14 days of the experiment	644:677	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	0	46	theme	haemato-biochemical	94:112	arg1	parameters					114:123	haemato-biochemical parameters	94:123	haemato-biochemical parameters	94:123	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	4	47	theme	Dietary	767:773	arg1	ZEO20					775:779	Dietary ZEO20	767:779	Dietary ZEO20	767:779	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	12	48	theme	other	1951:1955	arg1	groups					1957:1962	the other groups	1947:1962	the other groups	1947:1962	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	6	49	theme	dry	1088:1090	arg1	matter					1092:1097	dry matter	1088:1097	dry matter	1088:1097	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	8	50	contain	had	1375:1377	arg2	increments					1391:1400	significant increments	1379:1400	significant increments in the MCV values (P ˂ 0.05)	1379:1429	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	8	50	contain	had	1375:1377	arg1	diets					1347:1351	Fish fed diets	1338:1351	Fish fed diets with ChNPs and/or ZEO	1338:1373	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	8	51	theme	˂	1423:1423	arg1	P					1421:1421	P ˂ 0.05	1421:1428	P ˂ 0.05	1421:1428	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	8	51	theme	˂	1423:1423	arg1	values					1413:1418	the MCV values	1405:1418	the MCV values (P ˂ 0.05)	1405:1429	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	6	52	theme	moisture	1078:1085	arg1	percentages					1108:1118	the moisture, dry matter, and ash percentages	1074:1118	the moisture, dry matter, and ash percentages	1074:1118	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	5	53	theme	fish	903:906	arg1	body					908:911	the fish body	899:911	the fish body	899:911	The crude protein of the fish body was significantly increased in all groups compared to the CTR (P ˂ 0.05).					
33560509	2	54	theme	0.01	428:431	arg1	±					426:426	±	426:426	±	426:426	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	10	55	theme	˂	1678:1678	arg1	P					1676:1676	P ˂ 0.05	1676:1683	P ˂ 0.05	1676:1683	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	10	55	theme	˂	1678:1678	arg1	groups					1668:1673	all treated groups	1656:1673	all treated groups (P ˂ 0.05)	1656:1684	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	15	56	theme	leading	2316:2322	arg1	application					2248:2258	the application	2244:2258	the application of ZEO and/or ChNPs in Nile tilapia diets	2244:2300	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	15	56	theme	leading	2316:2322	arg1	approach					2324:2331	a leading approach	2314:2331	a leading approach to mitigate the toxic impacts of IMID	2314:2369	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	0	57	theme	clinoptilolite	23:36	arg1	nanoparticles					51:63	dietary clinoptilolite and chitosan nanoparticles	15:63	dietary clinoptilolite and chitosan nanoparticles	15:63	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	2	58	dep	groups	460:465	arg1	one					468:470	one	468:470	one	468:470	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	59	theme	18.03	420:424	arg1	±					426:426	±	426:426	±	426:426	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	12	60	theme	nitric	1915:1920	arg1	oxide					1922:1926	lowest nitric oxide	1908:1926	lowest nitric oxide	1908:1926	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	9	61	with	combination	1490:1500	arg1	ZEO					1507:1509	ZEO	1507:1509	ZEO	1507:1509	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	0	62	theme	chitosan	42:49	arg1	nanoparticles					51:63	dietary clinoptilolite and chitosan nanoparticles	15:63	dietary clinoptilolite and chitosan nanoparticles	15:63	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	5	63	theme	crude	882:886	arg1	protein					888:894	The crude protein	878:894	The crude protein of the fish body	878:911	The crude protein of the fish body was significantly increased in all groups compared to the CTR (P ˂ 0.05).					
33560509	7	64	theme	hematocrit	1142:1151	arg1	values					1153:1158	hematocrit values	1142:1158	hematocrit values	1142:1158	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	3	65	theme	IMID	728:731	arg1	dose					720:723	a sub-lethal dose	707:723	a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1)	707:764	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	12	66	theme	highest	1880:1886	arg1	malondialdehyde					1888:1902	malondialdehyde	1888:1902	malondialdehyde	1888:1902	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	7	67	dep	decreased	1179:1187	arg1	P					1190:1190	P ˂ 0.05	1190:1197	P ˂ 0.05	1190:1197	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	14	68	with	treatment	2199:2207	arg1	ZEO					2224:2226	ZEO	2224:2226	ZEO	2224:2226	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	14	68	with	treatment	2199:2207	arg1	ChNPs					2214:2218	ChNPs	2214:2218	ChNPs	2214:2218	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	0	69	theme	tilapia	177:183	arg1	responses					133:141	immune responses	126:141	immune responses	126:141	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	69	theme	tilapia	177:183	arg1	status					162:167	antioxidative status	148:167	antioxidative status	148:167	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	69	theme	tilapia	177:183	arg1	growth					68:73	growth	68:73	growth	68:73	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	69	theme	tilapia	177:183	arg1	composition					81:91	body composition	76:91	body composition	76:91	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	69	theme	tilapia	177:183	arg1	parameters					114:123	haemato-biochemical parameters	94:123	haemato-biochemical parameters	94:123	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	6	70	theme	ash	1104:1106	arg1	percentages					1108:1118	the moisture, dry matter, and ash percentages	1074:1118	the moisture, dry matter, and ash percentages	1074:1118	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	12	71	theme	CTR	1862:1864	arg1	group					1866:1870	the CTR group	1858:1870	the CTR group	1858:1870	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	15	72	theme	ChNPs	2274:2278	arg1	application					2248:2258	the application	2244:2258	the application of ZEO and/or ChNPs in Nile tilapia diets	2244:2300	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	15	72	theme	ChNPs	2274:2278	arg1	approach					2324:2331	a leading approach	2314:2331	a leading approach to mitigate the toxic impacts of IMID	2314:2369	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	1	73	theme	mitigating	252:261	arg1	effects					263:269	the mitigating effects	248:269	the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	248:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	15	74	theme	ZEO	2263:2265	arg1	application					2248:2258	the application	2244:2258	the application of ZEO and/or ChNPs in Nile tilapia diets	2244:2300	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	15	74	theme	ZEO	2263:2265	arg1	approach					2324:2331	a leading approach	2314:2331	a leading approach to mitigate the toxic impacts of IMID	2314:2369	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	9	75	dep	glucose	1526:1532	arg1	the					1515:1517	the	1515:1517	the	1515:1517	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	13	76	theme	ZEO	2076:2078	arg1	combination					2061:2071	a combination	2059:2071	a combination of ZEO and ChNPs	2059:2088	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	15	77	theme	IMID	2366:2369	arg1	impacts					2355:2361	the toxic impacts	2345:2361	the toxic impacts of IMID	2345:2369	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	11	78	theme	tetrazolium	1800:1810	arg1	P					1820:1820	P ˂ 0.05	1820:1827	P ˂ 0.05	1820:1827	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	11	78	theme	tetrazolium	1800:1810	arg1	values					1812:1817	the highest lysozyme and nitro blue tetrazolium values	1764:1817	the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05)	1764:1828	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	8	79	with	diets	1347:1351	arg1	ZEO					1371:1373	ZEO	1371:1373	ZEO	1371:1373	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	8	79	with	diets	1347:1351	arg1	ChNPs					1358:1362	ChNPs	1358:1362	ChNPs	1358:1362	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	0	80	theme	immune	126:131	arg1	responses					133:141	immune responses	126:141	immune responses	126:141	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	3	81	dep	h	742:742	arg1	96					739:740	96	739:740	96	739:740	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	11	82	theme	˂	1822:1822	arg1	P					1820:1820	P ˂ 0.05	1820:1827	P ˂ 0.05	1820:1827	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	11	82	theme	˂	1822:1822	arg1	values					1812:1817	the highest lysozyme and nitro blue tetrazolium values	1764:1817	the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05)	1764:1828	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	9	83	contain	had	1511:1513	arg2	levels					1559:1564	the lowest glucose and alkaline phosphatase levels	1515:1564	the lowest glucose and alkaline phosphatase levels	1515:1564	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	9	83	contain	had	1511:1513	arg1	fish					1442:1445	fish	1442:1445	fish fed diets supplemented with ChNPs or their combination with ZEO	1442:1509	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	1	84	theme	-induced	355:362	arg1	toxicity					364:371	imidacloprid (IMID)-induced toxicity	336:371	imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	336:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	3	85	dep	=	749:749	arg1	L-1					761:763	0.0545 μg L-1	751:763	0.0545 μg L-1	751:763	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	0	86	theme	antioxidative	148:160	arg1	status					162:167	antioxidative status	148:167	antioxidative status	148:167	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	8	87	theme	fed	1343:1345	arg1	diets					1347:1351	Fish fed diets	1338:1351	Fish fed diets with ChNPs and/or ZEO	1338:1373	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	14	88	theme	complete	2102:2109	arg1	protection of					2111:2123	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	13	89	theme	lowest	1984:1989	arg1	residues					1996:2003	the lowest IMID residues	1980:2003	the lowest IMID residues in fish	1980:2011	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	9	90	theme	lowest	1519:1524	arg1	glucose					1526:1532	glucose	1526:1532	glucose	1526:1532	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	5	91	theme	˂	978:978	arg1	P					976:976	P ˂ 0.05	976:983	P ˂ 0.05	976:983	The crude protein of the fish body was significantly increased in all groups compared to the CTR (P ˂ 0.05).					
33560509	5	91	theme	˂	978:978	arg1	CTR					971:973	the CTR	967:973	the CTR (P ˂ 0.05)	967:984	The crude protein of the fish body was significantly increased in all groups compared to the CTR (P ˂ 0.05).					
33560509	6	92	theme	significant	990:1000	arg1	impacts					1002:1008	No significant impacts	987:1008	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05)	987:1056	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	14	93	theme	Partial	2091:2097	arg1	protection of					2111:2123	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues	2091:2155	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	2	94	theme	basal	481:485	arg1	CTR					503:505	CTR	503:505	CTR	503:505	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	94	theme	basal	481:485	arg1	diet					487:490	a basal diet	479:490	a basal diet (control) (CTR)	479:506	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	2	94	theme	basal	481:485	arg1	control					493:499	control	493:499	control	493:499	Fish (18.03 ± 0.01 g) were allocated into six groups; one fed on a basal diet (control) (CTR), and the other groups were fed diets supplemented with ChNPs (5 g kg-1) and/or ZEO (20 and 40 g kg-1) (ZEO20 and ZEO40) for 60 days.					
33560509	7	95	dep	increased	1269:1277	arg1	P					1280:1280	P ˂ 0.05	1280:1287	P ˂ 0.05	1280:1287	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	8	96	from	increments	1391:1400	arg1	P					1421:1421	P ˂ 0.05	1421:1428	P ˂ 0.05	1421:1428	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	8	96	from	increments	1391:1400	arg1	values					1413:1418	the MCV values	1405:1418	the MCV values (P ˂ 0.05)	1405:1429	Fish fed diets with ChNPs and/or ZEO had significant increments in the MCV values (P ˂ 0.05).					
33560509	1	97	theme	Oreochromis	390:400	arg1	tilapia					381:387	Nile tilapia	376:387	Nile tilapia (Oreochromis niloticus)	376:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	97	theme	Oreochromis	390:400	arg1	niloticus					402:410	Oreochromis niloticus	390:410	Oreochromis niloticus	390:410	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	11	98	theme	highest	1768:1774	arg1	lysozyme					1776:1783	the highest lysozyme	1764:1783	the highest lysozyme	1764:1783	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	9	99	theme	phosphatase	1547:1557	arg1	levels					1559:1564	the lowest glucose and alkaline phosphatase levels	1515:1564	the lowest glucose and alkaline phosphatase levels	1515:1564	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	0	100	theme	body	76:79	arg1	composition					81:91	body composition	76:91	body composition	76:91	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	13	101	from	residues	1996:2003	arg1	fish					2008:2011	fish	2008:2011	fish	2008:2011	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	1	102	from	effects	263:269	arg1	toxicity					364:371	imidacloprid (IMID)-induced toxicity	336:371	imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	336:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	0	103	from	effects	4:10	arg1	responses					133:141	immune responses	126:141	immune responses	126:141	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	103	from	effects	4:10	arg1	status					162:167	antioxidative status	148:167	antioxidative status	148:167	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	103	from	effects	4:10	arg1	growth					68:73	growth	68:73	growth	68:73	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	103	from	effects	4:10	arg1	composition					81:91	body composition	76:91	body composition	76:91	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	0	103	from	effects	4:10	arg1	parameters					114:123	haemato-biochemical parameters	94:123	haemato-biochemical parameters	94:123	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	12	104	contain	had	1872:1874	arg1	fish					1850:1853	fish	1850:1853	fish in the CTR group	1850:1870	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	12	104	contain	had	1872:1874	arg2	malondialdehyde					1888:1902	malondialdehyde	1888:1902	malondialdehyde	1888:1902	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	12	104	contain	had	1872:1874	arg2	oxide					1922:1926	lowest nitric oxide	1908:1926	lowest nitric oxide	1908:1926	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
33560509	10	105	theme	aspartate	1595:1603	arg1	levels					1617:1622	Serum aspartate transferase levels	1589:1622	Serum aspartate transferase levels	1589:1622	Serum aspartate transferase levels were significantly decreased in all treated groups (P ˂ 0.05) compared to the CTR.					
33560509	1	106	from	toxicity	364:371	arg1	tilapia					381:387	Nile tilapia	376:387	Nile tilapia (Oreochromis niloticus)	376:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	106	from	toxicity	364:371	arg1	niloticus					402:410	Oreochromis niloticus	390:410	Oreochromis niloticus	390:410	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	106	from	toxicity	364:371	arg1	evaluation					234:243	the evaluation	230:243	the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus)	230:411	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	3	107	theme	experiment	668:677	arg1	days					656:659	the last 14 days	644:659	the last 14 days of the experiment	644:677	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	11	108	theme	blue	1795:1798	arg1	tetrazolium					1800:1810	nitro blue tetrazolium	1789:1810	nitro blue tetrazolium	1789:1810	ChNPs alone or combined with ZEO significantly exhibited the highest lysozyme and nitro blue tetrazolium values (P ˂ 0.05).					
33560509	3	109	dep	LC50	744:747	arg1	h					742:742	h	742:742	h	742:742	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	13	110	theme	IMID	1991:1994	arg1	residues					1996:2003	the lowest IMID residues	1980:2003	the lowest IMID residues in fish	1980:2011	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	6	111	theme	matter	1092:1097	arg1	percentages					1108:1118	the moisture, dry matter, and ash percentages	1074:1118	the moisture, dry matter, and ash percentages	1074:1118	No significant impacts of ChNPs, ZEO, and their interaction (P > 0.05) were noticed on the moisture, dry matter, and ash percentages.					
33560509	15	112	from	application	2248:2258	arg1	diets					2296:2300	Nile tilapia diets	2283:2300	Nile tilapia diets	2283:2300	In conclusion, the application of ZEO and/or ChNPs in Nile tilapia diets looks to be a leading approach to mitigate the toxic impacts of IMID.					
33560509	0	113	theme	dietary	15:21	arg1	nanoparticles					51:63	dietary clinoptilolite and chitosan nanoparticles	15:63	dietary clinoptilolite and chitosan nanoparticles	15:63	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	1	114	theme	dietary	274:280	arg1	zeolites					282:289	dietary zeolites	274:289	dietary zeolites (ZEO)	274:295	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	114	theme	dietary	274:280	arg1	ZEO					292:294	ZEO	292:294	ZEO	292:294	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	7	115	theme	˂	1282:1282	arg1	P					1280:1280	P ˂ 0.05	1280:1287	P ˂ 0.05	1280:1287	Compared to the CTR, hematocrit values were significantly decreased (P ˂ 0.05) in ChNP and ZEO20 groups; meanwhile, their levels were significantly increased (P ˂ 0.05) in the ZEO40 group and all combined treatments.					
33560509	5	116	theme	body	908:911	arg1	protein					888:894	The crude protein	878:894	The crude protein of the fish body	878:911	The crude protein of the fish body was significantly increased in all groups compared to the CTR (P ˂ 0.05).					
33560509	3	117	theme	sub-lethal	709:718	arg1	dose					720:723	a sub-lethal dose	707:723	a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1)	707:764	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	0	118	theme	nanoparticles	51:63	arg1	effects					4:10	The effects	0:10	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia	0:183	The effects of dietary clinoptilolite and chitosan nanoparticles on growth, body composition, haemato-biochemical parameters, immune responses, and antioxidative status of Nile tilapia exposed to imidacloprid.					
33560509	13	119	dep	flesh were	2013:2022	arg1	found					2024:2028	found	2024:2028	flesh were found in fish groups fed diet with a combination of ZEO and ChNPs	2013:2088	Interestingly, the lowest IMID residues in fish flesh were found in fish groups fed diet with a combination of ZEO and ChNPs.					
33560509	9	120	with	ChNPs	1475:1479	arg1	ZEO					1507:1509	ZEO	1507:1509	ZEO	1507:1509	Moreover, fish fed diets supplemented with ChNPs or their combination with ZEO had the lowest glucose and alkaline phosphatase levels compared with the CTR.					
33560509	1	121	theme	chitosan	304:311	arg1	ChNP					327:330	ChNP	327:330	ChNP	327:330	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	1	121	theme	chitosan	304:311	arg1	nanoparticle					313:324	chitosan nanoparticle	304:324	chitosan nanoparticle (ChNP)	304:331	This study aimed at the evaluation of the mitigating effects of dietary zeolites (ZEO) and/or chitosan nanoparticle (ChNP) on imidacloprid (IMID)-induced toxicity in Nile tilapia (Oreochromis niloticus).					
33560509	4	122	contain	had	850:852	arg2	effects					869:875	no significant effects	854:875	no significant effects	854:875	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	4	122	contain	had	850:852	arg1	ChNPs					844:848	ChNPs	844:848	ChNPs	844:848	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	14	123	theme	with combined	2185:2197	arg1	treatment					2199:2207	fish group with combined treatment	2174:2207	fish group with combined treatment with ChNPs and ZEO	2174:2226	Partial or complete protection of the hepatic and splenic tissues were observed in fish group with combined treatment with ChNPs and ZEO.					
33560509	3	124	theme	=	749:749	arg1	LC50					744:747	½ of 96 h LC50	734:747	½ of 96 h LC50 = 0.0545 μg L-1	734:763	In the last 14 days of the experiment, all groups were exposed to a sub-lethal dose of IMID (½ of 96 h LC50 = 0.0545 μg L-1).					
33560509	4	125	theme	˂	829:829	arg1	P					827:827	P ˂ 0.05	827:834	P ˂ 0.05	827:834	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	4	125	theme	˂	829:829	arg1	parameters					815:824	all growth parameters	804:824	all growth parameters (P ˂ 0.05)	804:835	Dietary ZEO20 significantly improved all growth parameters (P ˂ 0.05), while ChNPs had no significant effects.					
33560509	12	126	theme	lowest	1908:1913	arg1	oxide					1922:1926	lowest nitric oxide	1908:1926	lowest nitric oxide	1908:1926	On the other hand, fish in the CTR group had the highest malondialdehyde and lowest nitric oxide levels compared to the other groups.					
34346569	0	0	theme	wound	114:118	arg1	healing					120:126	wound healing	114:126	wound healing	114:126	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	6	1	theme	nanocomposite	1463:1475	arg1	strength					1447:1454	good strength	1442:1454	good strength of the nanocomposite	1442:1475	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	6	2	theme	PVA/BC/g-C3	1369:1379	arg1	nanofibers					1407:1416	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1369:1416	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1369:1416	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	5	3	theme	cell	977:980	arg1	proliferation					982:994	cell proliferation	977:994	cell proliferation	977:994	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	4	theme	N4	1132:1133	arg1	composite					1158:1166	the BC/PVA/g-C3 N4 /nettles/trachyspermum composite	1116:1166	the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation	1116:1277	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	5	theme	proliferation	982:994	arg1	results					996:1002	the toxicity, migration, and cell proliferation results	948:1002	the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts	948:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	6	from	fibroblasts	1044:1054	arg1	results					996:1002	the toxicity, migration, and cell proliferation results	948:1002	the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts	948:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	1	7	theme	high	272:275	arg1	porosity					277:284	high porosity	272:284	high porosity	272:284	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	7	8	theme	wound	1552:1556	arg1	healing					1558:1564	wound healing	1552:1564	wound healing	1552:1564	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	3	9	theme	N4	597:598	arg1	solution					600:607	g-C3 N4 solution	592:607	g-C3 N4 solution	592:607	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	5	10	theme	3T3	1040:1042	arg1	fibroblasts					1044:1054	NIH 3T3 fibroblasts	1036:1054	NIH 3T3 fibroblasts	1036:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	11	theme	toxic	1184:1188	arg1	effect					1190:1195	no toxic effect	1181:1195	no toxic effect	1181:1195	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	7	12	theme	produced	1510:1517	arg1	nanofibers					1519:1528	the produced nanofibers	1506:1528	the produced nanofibers	1506:1528	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	5	13	theme	MTT	1077:1079	arg1	assays					1093:1098	MTT and scratch assays	1077:1098	assays	1093:1098	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	14	theme	synthesized	1011:1021	arg1	materials					1023:1031	the synthesized materials	1007:1031	the synthesized materials on NIH 3T3 fibroblasts	1007:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	6	15	theme	good	1442:1445	arg1	strength					1447:1454	good strength	1442:1454	good strength of the nanocomposite	1442:1475	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	6	16	theme	strength	1338:1345	arg1	test					1347:1350	a tensile strength test	1328:1350	a tensile strength test	1328:1350	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	4	17	theme	gram-positive	847:859	arg1	bacteria					879:886	gram-positive and gram-negative bacteria	847:886	gram-positive and gram-negative bacteria using the minimum inhibitory concentration method	847:936	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	1	18	theme	attention	185:193	arg1	deal					177:180	a great deal	169:180	a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility	169:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	7	19	used	used	1535:1538	arg2	nanofibers					1519:1528	the produced nanofibers	1506:1528	the produced nanofibers	1506:1528	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	6	20	theme	tensile	1330:1336	arg1	test					1347:1350	a tensile strength test	1328:1350	a tensile strength test	1328:1350	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	7	21	theme	healing	1695:1701	arg1	percentage					1703:1712	the wound healing percentage	1685:1712	the wound healing percentage	1685:1712	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	0	22	theme	PVA	0:2	arg1	nanofiber					10:18	PVA based nanofiber	0:18	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum	0:100	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	8	23	theme	N4	1771:1772	arg1	nanofibers					1797:1806	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1759:1806	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1759:1806	Therefore, this study shows that PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.					
34346569	2	24	theme	cellulose/graphitic	393:411	arg1	nanocomposite					450:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	0	25	theme	based	4:8	arg1	nanofiber					10:18	PVA based nanofiber	0:18	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum	0:100	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	7	26	theme	wound	1689:1693	arg1	healing					1695:1701	the wound healing	1685:1701	the wound healing percentage	1685:1712	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	0	27	contain	containing	20:29	arg1	nanofiber					10:18	PVA based nanofiber	0:18	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum	0:100	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	0	27	contain	containing	20:29	arg2	cellulose					31:39	cellulose	31:39	cellulose modified with graphitic carbon nitride/nettles/trachyspermum	31:100	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	5	28	theme	materials	1023:1031	arg1	results					996:1002	the toxicity, migration, and cell proliferation results	948:1002	the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts	948:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	8	29	theme	PVA/BC/g-C3	1759:1769	arg1	nanofibers					1797:1806	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1759:1806	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1759:1806	Therefore, this study shows that PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.					
34346569	2	30	theme	bacterial	383:391	arg1	nanocomposite					450:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	2	31	theme	N4	493:494	arg1	nanofiber					519:527	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber	481:527	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber	481:527	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	1	32	theme	good	287:290	arg1	uptake					298:303	good fluid uptake	287:303	good fluid uptake	287:303	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	5	33	from	results	996:1002	arg1	fibroblasts					1044:1054	NIH 3T3 fibroblasts	1036:1054	NIH 3T3 fibroblasts	1036:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	34	theme	BC/PVA/g-C3	1120:1130	arg1	composite					1158:1166	the BC/PVA/g-C3 N4 /nettles/trachyspermum composite	1116:1166	the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation	1116:1277	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	1	35	theme	medical	203:209	arg1	applications					211:222	its medical applications	199:222	its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility	199:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	2	36	theme	PVA/BC/g-C3	481:491	arg1	nanofiber					519:527	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber	481:527	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber	481:527	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	1	37	theme	fluid	292:296	arg1	uptake					298:303	good fluid uptake	287:303	good fluid uptake	287:303	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	5	38	dep	toxicity	952:959	arg1	the					948:950	the	948:950	the	948:950	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	4	39	theme	materials	822:830	arg1	properties					792:801	the antibacterial properties	774:801	the antibacterial properties of the synthesized materials	774:830	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	8	40	theme	/nettles/trachyspermum	1774:1795	arg1	nanofibers					1797:1806	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1759:1806	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1759:1806	Therefore, this study shows that PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.					
34346569	3	41	dep	transform	668:676	arg1	infrared					678:685	infrared	678:685	transform infrared	668:685	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	7	42	theme	vivo	1494:1497	arg1	assay					1499:1503	vivo assay	1494:1503	vivo assay	1494:1503	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	3	43	theme	Zeta	707:710	arg1	Potential					712:720	Zeta Potential	707:720	Zeta Potential	707:720	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	4	44	theme	synthesized	810:820	arg1	materials					822:830	the synthesized materials	806:830	the synthesized materials	806:830	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	1	45	theme	due	224:226	arg1	applications					211:222	its medical applications	199:222	its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility	199:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	2	46	theme	/nettles/trachyspermum	496:517	arg1	nanofiber					519:527	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber	481:527	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber	481:527	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	4	47	theme	minimum	898:904	arg1	method					931:936	the minimum inhibitory concentration method	894:936	the minimum inhibitory concentration method	894:936	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	3	48	dep	Fourier	660:666	arg1	analyzes					758:765	analyzes	758:765	analyzes	758:765	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	3	48	dep	Fourier	660:666	arg1	transform					668:676	transform	668:676	transform infrared	668:685	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	2	49	theme	carbon	413:418	arg1	nanocomposite					450:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	1	50	theme	good	306:309	arg1	strength					311:318	good strength	306:318	good strength	306:318	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	5	51	dep	composite	1158:1166	arg1	contributed					1215:1225	contributed	1215:1225	not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation	1168:1277	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	5	51	dep	composite	1158:1166	arg1	had					1177:1179	had	1177:1179	not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation	1168:1277	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	6	52	theme	mechanical	1305:1314	arg1	properties					1316:1325	the mechanical properties	1301:1325	the mechanical properties	1301:1325	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	2	53	theme	nitride/nettles/trachyspermum	420:448	arg1	nanocomposite					450:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite	383:462	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	0	54	theme	graphitic	55:63	arg1	nitride/nettles/trachyspermum					72:100	graphitic carbon nitride/nettles/trachyspermum	55:100	graphitic carbon nitride/nettles/trachyspermum	55:100	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	5	55	theme	cell	1230:1233	arg1	survival					1235:1242	cell survival	1230:1242	cell survival	1230:1242	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	0	56	theme	carbon	65:70	arg1	nitride/nettles/trachyspermum					72:100	graphitic carbon nitride/nettles/trachyspermum	55:100	graphitic carbon nitride/nettles/trachyspermum	55:100	PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing.					
34346569	4	57	theme	concentration	917:929	arg1	method					931:936	the minimum inhibitory concentration method	894:936	the minimum inhibitory concentration method	894:936	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	8	58	theme	bacterial	1828:1836	arg1	growth					1838:1843	bacterial growth	1828:1843	bacterial growth	1828:1843	Therefore, this study shows that PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.					
34346569	1	59	theme	bacterial	136:144	arg1	cellulose					146:154	bacterial cellulose	136:154	bacterial cellulose	136:154	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	5	60	theme	NIH	1036:1038	arg1	fibroblasts					1044:1054	NIH 3T3 fibroblasts	1036:1054	NIH 3T3 fibroblasts	1036:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	6	61	theme	/nettles/trachyspermum	1384:1405	arg1	nanofibers					1407:1416	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1369:1416	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1369:1416	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	3	62	theme	scanning	727:734	arg1	microscope					747:756	scanning electronic microscope	727:756	scanning electronic microscope	727:756	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	4	63	theme	inhibitory	906:915	arg1	method					931:936	the minimum inhibitory concentration method	894:936	the minimum inhibitory concentration method	894:936	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	5	64	theme	cell	1245:1248	arg1	migration					1250:1258	cell migration	1245:1258	cell migration	1245:1258	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	6	65	theme	N4	1381:1382	arg1	nanofibers					1407:1416	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1369:1416	PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers	1369:1416	To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite.					
34346569	3	66	theme	electronic	736:745	arg1	microscope					747:756	scanning electronic microscope	727:756	scanning electronic microscope	727:756	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	4	67	theme	gram-negative	865:877	arg1	bacteria					879:886	gram-positive and gram-negative bacteria	847:886	gram-positive and gram-negative bacteria using the minimum inhibitory concentration method	847:936	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	5	68	theme	migration	962:970	arg1	results					996:1002	the toxicity, migration, and cell proliferation results	948:1002	the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts	948:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	1	69	theme	unique	235:240	arg1	properties					253:262	its unique structural properties	231:262	its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility	231:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	69	theme	unique	235:240	arg1	strength					311:318	good strength	306:318	good strength	306:318	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	69	theme	unique	235:240	arg1	biocompatibility					325:340	biocompatibility	325:340	biocompatibility	325:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	69	theme	unique	235:240	arg1	porosity					277:284	high porosity	272:284	high porosity	272:284	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	69	theme	unique	235:240	arg1	uptake					298:303	good fluid uptake	287:303	good fluid uptake	287:303	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	3	70	theme	g-C3	592:595	arg1	solution					600:607	g-C3 N4 solution	592:607	g-C3 N4 solution	592:607	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	5	71	theme	/nettles/trachyspermum	1135:1156	arg1	composite					1158:1166	the BC/PVA/g-C3 N4 /nettles/trachyspermum composite	1116:1166	the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation	1116:1277	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	3	72	theme	X-ray	688:692	arg1	diffraction					694:704	X-ray diffraction	688:704	X-ray diffraction	688:704	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	4	73	theme	antibacterial	778:790	arg1	properties					792:801	the antibacterial properties	774:801	the antibacterial properties of the synthesized materials	774:830	Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method.					
34346569	5	74	from	materials	1023:1031	arg1	fibroblasts					1044:1054	NIH 3T3 fibroblasts	1036:1054	NIH 3T3 fibroblasts	1036:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	1	75	theme	structural	242:251	arg1	properties					253:262	its unique structural properties	231:262	its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility	231:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	75	theme	structural	242:251	arg1	strength					311:318	good strength	306:318	good strength	306:318	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	75	theme	structural	242:251	arg1	biocompatibility					325:340	biocompatibility	325:340	biocompatibility	325:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	75	theme	structural	242:251	arg1	porosity					277:284	high porosity	272:284	high porosity	272:284	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	1	75	theme	structural	242:251	arg1	uptake					298:303	good fluid uptake	287:303	good fluid uptake	287:303	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	5	76	theme	scratch	1085:1091	arg1	assays					1093:1098	MTT and scratch assays	1077:1098	assays	1093:1098	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	8	77	theme	wound	1860:1864	arg1	healing					1866:1872	wound healing	1860:1872	wound healing	1860:1872	Therefore, this study shows that PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.					
34346569	3	78	theme	g-C3	580:583	arg1	N4					585:586	The g-C3 N4	576:586	The g-C3 N4	576:586	The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes.					
34346569	5	79	theme	toxicity	952:959	arg1	results					996:1002	the toxicity, migration, and cell proliferation results	948:1002	the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts	948:1054	Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done.					
34346569	7	80	theme	wound	1640:1644	arg1	healing					1646:1652	wound healing	1640:1652	the wound healing process so that after 14 days, the wound healing percentage showed 95%	1636:1723	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
34346569	2	81	theme	wound	560:564	arg1	dressing					566:573	a wound dressing	558:573	a wound dressing	558:573	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	2	81	theme	wound	560:564	arg1	method					548:553	method	548:553	method	548:553	This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing.					
34346569	1	82	theme	great	171:175	arg1	deal					177:180	a great deal	169:180	a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility	169:340	Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility.					
34346569	7	83	theme	healing	1646:1652	arg1	process					1654:1660	the wound healing process	1636:1660	the wound healing process so that after 14 days, the wound healing percentage showed 95%	1636:1723	In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%.					
33838793	8	0	theme	food	1140:1143	arg1	treatment					1145:1153	food treatment	1140:1153	food treatment	1140:1153	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	3	1	theme	grafting	514:521	arg1	reaction					523:530	grafting reaction	514:530	grafting reaction	514:530	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	8	2	theme	bio-based	1054:1062	arg1	beads					1074:1078	the bio-based composite beads	1050:1078	the bio-based composite beads	1050:1078	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	6	3	theme	cellulose	799:807	arg1	beads					809:813	The grafted cellulose beads	787:813	The grafted cellulose beads	787:813	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	5	4	theme	cellulose	720:728	arg1	I					730:730	cellulose I	720:730	cellulose I to cellulose II	720:746	The crystalline form of cellulose transformed from cellulose I to cellulose II after being dissolved and regenerated.					
33838793	6	5	theme	grafted	791:797	arg1	beads					809:813	The grafted cellulose beads	787:813	The grafted cellulose beads	787:813	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	3	6	theme	formed	559:564	arg1	groups					575:580	newly formed carboxyl groups	553:580	newly formed carboxyl groups of cellulose and amino of EPL	553:610	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	6	theme	formed	559:564	arg1	amino					599:603	amino	599:603	amino	599:603	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	6	theme	formed	559:564	arg1	cellulose					585:593	cellulose	585:593	cellulose	585:593	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	2	7	dep	mediated	364:371	arg1	2					316:316	2	316:316	2	316:316	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	2	7	dep	mediated	364:371	arg1	6-tetramethylpiperidine-1-oxyl					325:354	6-tetramethylpiperidine-1-oxyl	325:354	6-tetramethylpiperidine-1-oxyl	325:354	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	1	8	theme	ecological	182:191	arg1	environment					193:203	ecological environment	182:203	ecological environment	182:203	In recent years, harmful microorganisms in water pose great harm to ecological environment and human health.					
33838793	8	9	theme	composite	1064:1072	arg1	beads					1074:1078	the bio-based composite beads	1050:1078	the bio-based composite beads	1050:1078	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	2	10	theme	cellulose	282:290	arg1	beads					292:296	epsilon-poly-l-lysine (EPL) grafted cellulose beads	246:296	epsilon-poly-l-lysine (EPL) grafted cellulose beads	246:296	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	2	11	theme	carbodiimide	387:398	arg1	reaction					423:430	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction	316:430	reaction	423:430	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	8	12	theme	water	1117:1121	arg1	purification					1123:1134	water purification	1117:1134	water purification	1117:1134	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	1	13	theme	recent	117:122	arg1	years					124:128	recent years	117:128	recent years	117:128	In recent years, harmful microorganisms in water pose great harm to ecological environment and human health.					
33838793	1	14	theme	human	209:213	arg1	health					215:220	human health	209:220	human health	209:220	In recent years, harmful microorganisms in water pose great harm to ecological environment and human health.					
33838793	1	15	from	microorganisms	139:152	arg1	water					157:161	water	157:161	water	157:161	In recent years, harmful microorganisms in water pose great harm to ecological environment and human health.					
33838793	3	16	theme	carboxyl	566:573	arg1	groups					575:580	newly formed carboxyl groups	553:580	newly formed carboxyl groups of cellulose and amino of EPL	553:610	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	16	theme	carboxyl	566:573	arg1	amino					599:603	amino	599:603	amino	599:603	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	16	theme	carboxyl	566:573	arg1	cellulose					585:593	cellulose	585:593	cellulose	585:593	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	0	17	theme	antibacterial	21:33	arg1	properties					35:44	antibacterial properties	21:44	antibacterial properties	21:44	Characterization and antibacterial properties of epsilon-poly- l-lysine grafted multi-functional cellulose beads.					
33838793	8	18	theme	potential	1091:1099	arg1	applications					1101:1112	potential applications	1091:1112	potential applications	1091:1112	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	0	19	theme	epsilon-poly-	49:61	arg1	l-lysine					63:70	epsilon-poly- l-lysine	49:70	epsilon-poly- l-lysine	49:70	Characterization and antibacterial properties of epsilon-poly- l-lysine grafted multi-functional cellulose beads.					
33838793	5	20	theme	cellulose	693:701	arg1	form					685:688	The crystalline form	669:688	The crystalline form of cellulose	669:701	The crystalline form of cellulose transformed from cellulose I to cellulose II after being dissolved and regenerated.					
33838793	3	21	theme	cellulose	458:466	arg1	C6					452:453	C6	452:453	C6 of cellulose	452:466	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	2	22	theme	epsilon-poly-l-lysine	246:266	arg1	beads					292:296	epsilon-poly-l-lysine (EPL) grafted cellulose beads	246:296	epsilon-poly-l-lysine (EPL) grafted cellulose beads	246:296	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	3	23	theme	cellulose	585:593	arg1	groups					575:580	newly formed carboxyl groups	553:580	newly formed carboxyl groups of cellulose and amino of EPL	553:610	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	23	theme	cellulose	585:593	arg1	amino					599:603	amino	599:603	amino	599:603	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	23	theme	cellulose	585:593	arg1	cellulose					585:593	cellulose	585:593	cellulose	585:593	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	2	24	theme	mediated	400:407	arg1	reaction					423:430	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction	316:430	reaction	423:430	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	6	25	dep	Gram-negative	860:872	arg1	Escherichia					874:884	Escherichia	874:884	Escherichia	874:884	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	6	25	dep	Gram-negative	860:872	arg1	Gram-positive					892:904	Gram-positive	892:904	Gram-positive	892:904	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	3	26	theme	Hydroxyl	433:440	arg1	groups					442:447	Hydroxyl groups	433:447	Hydroxyl groups on C6 of cellulose	433:466	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	27	theme	EPL	608:610	arg1	amino					599:603	amino	599:603	amino	599:603	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	27	theme	EPL	608:610	arg1	cellulose					585:593	cellulose	585:593	cellulose	585:593	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	2	28	theme	cross-linking	409:421	arg1	reaction					423:430	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction	316:430	reaction	423:430	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	1	29	theme	harmful	131:137	arg1	microorganisms					139:152	harmful microorganisms	131:152	harmful microorganisms in water	131:161	In recent years, harmful microorganisms in water pose great harm to ecological environment and human health.					
33838793	0	30	theme	l-lysine	63:70	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antibacterial properties of epsilon-poly- l-lysine grafted multi-functional cellulose beads.					
33838793	0	30	theme	l-lysine	63:70	arg1	properties					35:44	antibacterial properties	21:44	antibacterial properties	21:44	Characterization and antibacterial properties of epsilon-poly- l-lysine grafted multi-functional cellulose beads.					
33838793	8	31	theme	purification	1123:1134	arg1	fields					1155:1160	water purification and food treatment fields	1117:1160	water purification and food treatment fields	1117:1160	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	3	32	theme	amino	599:603	arg1	groups					575:580	newly formed carboxyl groups	553:580	newly formed carboxyl groups of cellulose and amino of EPL	553:610	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	32	theme	amino	599:603	arg1	amino					599:603	amino	599:603	amino	599:603	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	3	32	theme	amino	599:603	arg1	cellulose					585:593	cellulose	585:593	cellulose	585:593	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	6	33	dep	Escherichia	874:884	arg1	coli					886:889	coli	886:889	coli	886:889	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	8	34	theme	treatment	1145:1153	arg1	fields					1155:1160	water purification and food treatment fields	1117:1160	water purification and food treatment fields	1117:1160	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	6	35	theme	antibacterial	827:839	arg1	activities					841:850	good antibacterial activities	822:850	good antibacterial activities	822:850	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	2	36	theme	grafted	274:280	arg1	beads					292:296	epsilon-poly-l-lysine (EPL) grafted cellulose beads	246:296	epsilon-poly-l-lysine (EPL) grafted cellulose beads	246:296	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	3	37	theme	carboxyl	485:492	arg1	groups					494:499	carboxyl groups	485:499	carboxyl groups	485:499	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	5	38	theme	crystalline	673:683	arg1	form					685:688	The crystalline form	669:688	The crystalline form of cellulose	669:701	The crystalline form of cellulose transformed from cellulose I to cellulose II after being dissolved and regenerated.					
33838793	6	39	theme	good	822:825	arg1	activities					841:850	good antibacterial activities	822:850	good antibacterial activities	822:850	The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h.					
33838793	3	40	from	groups	442:447	arg1	C6					452:453	C6	452:453	C6 of cellulose	452:466	Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL.					
33838793	0	41	theme	cellulose	97:105	arg1	beads					107:111	multi-functional cellulose beads	80:111	multi-functional cellulose beads	80:111	Characterization and antibacterial properties of epsilon-poly- l-lysine grafted multi-functional cellulose beads.					
33838793	2	42	theme	mediated	364:371	arg1	oxidation					373:381	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction	316:430	oxidation	373:381	To solve this problem, epsilon-poly-l-lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction.					
33838793	8	43	contain	have	1086:1089	arg1	beads					1074:1078	the bio-based composite beads	1050:1078	the bio-based composite beads	1050:1078	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	8	43	contain	have	1086:1089	arg2	applications					1101:1112	potential applications	1091:1112	potential applications	1091:1112	It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.					
33838793	0	44	theme	multi-functional	80:95	arg1	beads					107:111	multi-functional cellulose beads	80:111	multi-functional cellulose beads	80:111	Characterization and antibacterial properties of epsilon-poly- l-lysine grafted multi-functional cellulose beads.					
33838793	1	45	theme	great	168:172	arg1	harm					174:177	great harm	168:177	great harm	168:177	In recent years, harmful microorganisms in water pose great harm to ecological environment and human health.					
32600732	2	0	theme	mechanical	486:495	arg1	stability					497:505	mechanical stability	486:505	mechanical stability	486:505	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	1	1	contain	have	151:154	arg2	stability					172:180	good mechanical stability	156:180	good mechanical stability	156:180	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	1	1	contain	have	151:154	arg1	grafts					144:149	traditional synthetic vascular grafts	113:149	traditional synthetic vascular grafts	113:149	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	7	2	theme	potential	1235:1243	arg1	use					1245:1247	potential use	1235:1247	potential use in vascular repair	1235:1266	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	6	3	theme	important	1061:1069	arg1	factors					1071:1077	important factors	1061:1077	important factors involved in vascular remodelling and repair	1061:1121	It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair.					
32600732	2	4	theme	low	529:531	arg1	ratio					542:546	low swelling ratio	529:546	low swelling ratio	529:546	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	0	5	from	Research	0:7	arg1	alginate-polyacrylamide					12:34	alginate-polyacrylamide	12:34	alginate-polyacrylamide	12:34	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	6	6	from	HUVECs	1042:1047	arg1	secretion					1005:1013	the secretion	1001:1013	the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair	1001:1121	It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair.					
32600732	5	7	theme	ECs	941:943	arg1	migration					928:936	the migration	924:936	the migration of ECs to the surface of the hydrogel	924:974	What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel.					
32600732	4	8	theme	platelet	775:782	arg1	activation					761:770	activation	761:770	activation	761:770	Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis.					
32600732	4	8	theme	platelet	775:782	arg1	aggregation					745:755	aggregation	745:755	aggregation	745:755	Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis.					
32600732	4	8	theme	platelet	775:782	arg1	adhesion					735:742	adhesion	735:742	adhesion	735:742	Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis.					
32600732	3	9	contain	had	591:593	arg2	resistance					626:635	resistance	626:635	resistance to enzymatic degradation	626:660	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	3	9	contain	had	591:593	arg2	ability					685:691	anti-calcification ability	666:691	anti-calcification ability	666:691	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	3	9	contain	had	591:593	arg1	gel					587:589	the composite gel	573:589	the composite gel	573:589	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	3	9	contain	had	591:593	arg2	strength					616:623	excellent mechanical strength	595:623	excellent mechanical strength	595:623	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	5	10	theme	hydrogel	967:974	arg1	surface					952:958	the surface	948:958	the surface of the hydrogel	948:974	What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel.					
32600732	2	11	theme	high	508:511	arg1	bioactivity					513:523	high bioactivity	508:523	high bioactivity	508:523	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	4	12	theme	hemolysis	788:796	arg1	activation					761:770	activation	761:770	activation	761:770	Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis.					
32600732	4	12	theme	hemolysis	788:796	arg1	aggregation					745:755	aggregation	745:755	aggregation	745:755	Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis.					
32600732	4	12	theme	hemolysis	788:796	arg1	adhesion					735:742	adhesion	735:742	adhesion	735:742	Also, it could significantly inhibit the adhesion, aggregation and activation of platelet and hemolysis.					
32600732	2	13	theme	amnion	389:394	arg1	gel					396:398	acellular amnion gel and polyacrylamide-alginate gel	379:430	gel	396:398	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	2	14	theme	composite	445:453	arg1	hydrogel					455:462	a composite hydrogel	443:462	a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio	443:546	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	5	15	dep	adhesion	869:876	arg1	the					865:867	the	865:867	the	865:867	What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel.					
32600732	0	16	dep	material	94:101	arg1	substitute					83:92	substitute	83:92	substitute	83:92	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	2	17	theme	acellular	379:387	arg1	gel					396:398	acellular amnion gel and polyacrylamide-alginate gel	379:430	gel	396:398	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	5	18	theme	composite	818:826	arg1	hydrogel					828:835	this composite hydrogel	813:835	this composite hydrogel	813:835	What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel.					
32600732	5	19	theme	ECs	899:901	arg1	proliferation					882:894	proliferation	882:894	proliferation	882:894	What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel.					
32600732	5	19	theme	ECs	899:901	arg1	adhesion					869:876	adhesion	869:876	adhesion	869:876	What is more, this composite hydrogel could significantly promote the adhesion and proliferation of ECs, as well as inducing the migration of ECs to the surface of the hydrogel.					
32600732	1	20	theme	traditional	113:123	arg1	grafts					144:149	traditional synthetic vascular grafts	113:149	traditional synthetic vascular grafts	113:149	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	6	21	theme	seeded	1035:1040	arg1	HUVECs					1042:1047	seeded HUVECs	1035:1047	seeded HUVECs	1035:1047	It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair.					
32600732	6	22	theme	vascular	1091:1098	arg1	remodelling					1100:1110	vascular remodelling	1091:1110	vascular remodelling	1091:1110	It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair.					
32600732	1	23	theme	synthetic	125:133	arg1	grafts					144:149	traditional synthetic vascular grafts	113:149	traditional synthetic vascular grafts	113:149	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	1	24	theme	low	306:308	arg1	rate					324:327	low cell adhesion rate	306:327	low cell adhesion rate	306:327	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	6	25	theme	PGI2	1025:1028	arg1	secretion					1005:1013	the secretion	1001:1013	the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair	1001:1121	It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair.					
32600732	2	26	theme	polyacrylamide-alginate	404:426	arg1	gel					428:430	acellular amnion gel and polyacrylamide-alginate gel	379:430	gel	428:430	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	0	27	theme	amnion	45:50	arg1	hydrogel					52:59	amnion hydrogel	45:59	amnion hydrogel	45:59	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	0	27	theme	amnion	45:50	arg1	material					94:101	a potential vascular substitute material	62:101	a potential vascular substitute material	62:101	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	1	28	theme	cell	310:313	arg1	rate					324:327	low cell adhesion rate	306:327	low cell adhesion rate	306:327	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	2	29	theme	swelling	533:540	arg1	ratio					542:546	low swelling ratio	529:546	low swelling ratio	529:546	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	3	30	theme	excellent	595:603	arg1	strength					616:623	excellent mechanical strength	595:623	excellent mechanical strength	595:623	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	1	31	theme	adhesion	315:322	arg1	rate					324:327	low cell adhesion rate	306:327	low cell adhesion rate	306:327	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	3	32	theme	enzymatic	640:648	arg1	degradation					650:660	enzymatic degradation	640:660	enzymatic degradation	640:660	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	3	33	theme	composite	577:585	arg1	gel					587:589	the composite gel	573:589	the composite gel	573:589	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	1	34	theme	vascular	135:142	arg1	grafts					144:149	traditional synthetic vascular grafts	113:149	traditional synthetic vascular grafts	113:149	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	7	35	theme	excellent	1193:1201	arg1	hydrogel					1177:1184	prepared AlgSr/PAM-AM hydrogel	1155:1184	prepared AlgSr/PAM-AM hydrogel	1155:1184	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	35	theme	excellent	1193:1201	arg1	biomaterial					1203:1213	an excellent biomaterial	1190:1213	an excellent biomaterial with properties for potential use in vascular repair	1190:1266	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	36	theme	vascular	1252:1259	arg1	repair					1261:1266	vascular repair	1252:1266	vascular repair	1252:1266	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	37	with	biomaterial	1203:1213	arg1	properties					1220:1229	properties	1220:1229	properties	1220:1229	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	38	from	use	1245:1247	arg1	repair					1261:1266	vascular repair	1252:1266	vascular repair	1252:1266	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	6	39	theme	NO	1018:1019	arg1	secretion					1005:1013	the secretion	1001:1013	the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair	1001:1121	It could also stimulate the secretion of NO and PGI2 from seeded HUVECs, which were important factors involved in vascular remodelling and repair.					
32600732	3	40	theme	anti-calcification	666:683	arg1	ability					685:691	anti-calcification ability	666:691	anti-calcification ability	666:691	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	2	41	theme	high	469:472	arg1	elasticity					474:483	high elasticity	469:483	high elasticity	469:483	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	1	42	theme	good	156:159	arg1	stability					172:180	good mechanical stability	156:180	good mechanical stability	156:180	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	0	43	theme	vascular	74:81	arg1	hydrogel					52:59	amnion hydrogel	45:59	amnion hydrogel	45:59	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	0	43	theme	vascular	74:81	arg1	material					94:101	a potential vascular substitute material	62:101	a potential vascular substitute material	62:101	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	3	44	theme	mechanical	605:614	arg1	strength					616:623	excellent mechanical strength	595:623	excellent mechanical strength	595:623	The results showed that the composite gel had excellent mechanical strength, resistance to enzymatic degradation and anti-calcification ability.					
32600732	2	45	with	hydrogel	455:462	arg1	stability					497:505	mechanical stability	486:505	mechanical stability	486:505	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	2	45	with	hydrogel	455:462	arg1	bioactivity					513:523	high bioactivity	508:523	high bioactivity	508:523	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	2	45	with	hydrogel	455:462	arg1	ratio					542:546	low swelling ratio	529:546	low swelling ratio	529:546	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	2	45	with	hydrogel	455:462	arg1	elasticity					474:483	high elasticity	469:483	high elasticity	469:483	In order to address these problems, by combining acellular amnion gel and polyacrylamide-alginate gel, we gained a composite hydrogel with high elasticity, mechanical stability, high bioactivity and low swelling ratio.					
32600732	1	46	theme	mechanical	161:170	arg1	stability					172:180	good mechanical stability	156:180	good mechanical stability	156:180	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
32600732	0	47	theme	potential	64:72	arg1	hydrogel					52:59	amnion hydrogel	45:59	amnion hydrogel	45:59	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	0	47	theme	potential	64:72	arg1	material					94:101	a potential vascular substitute material	62:101	a potential vascular substitute material	62:101	Research on alginate-polyacrylamide enhanced amnion hydrogel, a potential vascular substitute material.					
32600732	7	48	theme	prepared	1155:1162	arg1	hydrogel					1177:1184	prepared AlgSr/PAM-AM hydrogel	1155:1184	prepared AlgSr/PAM-AM hydrogel	1155:1184	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	48	theme	prepared	1155:1162	arg1	biomaterial					1203:1213	an excellent biomaterial	1190:1213	an excellent biomaterial with properties for potential use in vascular repair	1190:1266	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	49	theme	AlgSr/PAM-AM	1164:1175	arg1	hydrogel					1177:1184	prepared AlgSr/PAM-AM hydrogel	1155:1184	prepared AlgSr/PAM-AM hydrogel	1155:1184	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	7	49	theme	AlgSr/PAM-AM	1164:1175	arg1	biomaterial					1203:1213	an excellent biomaterial	1190:1213	an excellent biomaterial with properties for potential use in vascular repair	1190:1266	All the results indicated that prepared AlgSr/PAM-AM hydrogel was an excellent biomaterial with properties for potential use in vascular repair.					
32600732	1	50	theme	transplantation	251:265	arg1	beginning					238:246	the beginning	234:246	the beginning of transplantation due to the material's procoagulant and low cell adhesion rate	234:327	Although traditional synthetic vascular grafts have good mechanical stability, stenosis and even thrombus can be easily caused at the beginning of transplantation due to the material's procoagulant and low cell adhesion rate.					
33023477	12	0	theme	high-starch	1779:1789	arg1	content					1791:1797	high-starch content	1779:1797	high-starch content	1779:1797	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	11	1	theme	starch	1602:1607	arg1	biosynthesis					1609:1620	starch biosynthesis	1602:1620	starch biosynthesis in lotus seed	1602:1634	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	9	2	theme	expression	1156:1165	arg1	patterns					1167:1174	varying expression patterns	1148:1174	varying expression patterns	1148:1174	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	10	3	theme	starch	1330:1335	arg1	number					1320:1325	a number	1318:1325	a number of starch and sucrose metabolism pathway related genes	1318:1380	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	10	4	theme	related	1368:1374	arg1	genes					1376:1380	pathway related genes	1360:1380	pathway related genes	1360:1380	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	12	5	theme	new	1754:1756	arg1	varieties					1764:1772	new lotus varieties	1754:1772	new lotus varieties with high-starch content	1754:1797	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	8	6	theme	Selective	970:978	arg1	analysis					989:996	Selective pressure analysis	970:996	Selective pressure analysis	970:996	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	3	7	from	biosynthesis	474:485	arg1	plants					490:495	plants	490:495	plants	490:495	ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus.					
33023477	1	8	theme	amylose	174:180	arg1	proportion					160:169	a high proportion	153:169	a high proportion	153:169	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	12	9	theme	theoretical	1723:1733	arg1	basis					1735:1739	theoretical basis	1723:1739	theoretical basis for breeding new lotus varieties with high-starch content	1723:1797	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	5	10	theme	significant	795:805	arg1	starch					817:822	significant different starch	795:822	significant different starch content	795:830	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	1	11	theme	lotus	196:200	arg1	property					219:226	lotus seed a promising property	196:226	lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food	196:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	10	12	from	activity	1442:1449	arg1	seed					1479:1482	seed	1479:1482	seed	1479:1482	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	0	13	from	roles	64:68	arg1	biosynthesis					80:91	starch biosynthesis	73:91	starch biosynthesis	73:91	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	0	13	from	roles	64:68	arg1	seed					102:105	lotus seed	96:105	lotus seed	96:105	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	1	14	from	property	219:226	arg1	development					235:245	the development	231:245	the development of hypoglycemic and low-glycemic index functional food	231:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	12	15	theme	lotus	1692:1696	arg1	genes					1705:1709	lotus AGPase genes	1692:1709	lotus AGPase genes	1692:1709	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	12	16	from	understanding	1675:1687	arg1	genes					1705:1709	lotus AGPase genes	1692:1709	lotus AGPase genes	1692:1709	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	5	17	theme	Comparative	671:681	arg1	analysis					697:704	Comparative transcriptome analysis	671:704	Comparative transcriptome analysis	671:704	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	10	18	theme	AGPase	1435:1440	arg1	activity					1442:1449	increased AGPase activity	1425:1449	increased AGPase activity	1425:1449	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	0	19	theme	starch	73:78	arg1	biosynthesis					80:91	starch biosynthesis	73:91	starch biosynthesis	73:91	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	2	20	theme	seed-lotus	369:378	arg1	breeding					380:387	seed-lotus breeding	369:387	seed-lotus breeding	369:387	Currently, improving starch content is one of the major goals for seed-lotus breeding.					
33023477	10	21	dep	seed	1479:1482	arg1	lotus					1473:1477	lotus seed	1473:1482	lotus seed	1473:1482	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	2	22	theme	goals	359:363	arg1	one					342:344	one	342:344	one	342:344	Currently, improving starch content is one of the major goals for seed-lotus breeding.					
33023477	2	22	theme	goals	359:363	arg1	goals					359:363	the major goals	349:363	the major goals for seed-lotus breeding	349:387	Currently, improving starch content is one of the major goals for seed-lotus breeding.					
33023477	0	23	theme	Comprehensive	0:12	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of AGPase genes	0:37	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	1	24	theme	hypoglycemic	250:261	arg1	food					297:300	hypoglycemic and low-glycemic index functional food	250:300	hypoglycemic and low-glycemic index functional food	250:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	8	25	theme	vital	1044:1048	arg1	selection					1023:1031	purifying selection	1013:1031	purifying selection	1013:1031	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	8	25	theme	vital	1044:1048	arg1	force					1050:1054	a vital force	1042:1054	a vital force in the evolution of AGPase genes	1042:1087	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	5	26	theme	different	807:815	arg1	starch					817:822	significant different starch	795:822	significant different starch content	795:830	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	1	27	theme	low-glycemic	267:278	arg1	food					297:300	hypoglycemic and low-glycemic index functional food	250:300	hypoglycemic and low-glycemic index functional food	250:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	3	28	theme	ADP-glucose	390:400	arg1	AGPase					421:426	AGPase	421:426	AGPase	421:426	ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus.					
33023477	3	28	theme	ADP-glucose	390:400	arg1	pyrophosphorylase					402:418	ADP-glucose pyrophosphorylase	390:418	ADP-glucose pyrophosphorylase (AGPase)	390:427	ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus.					
33023477	9	29	theme	Expression	1090:1099	arg1	analysis					1101:1108	Expression analysis	1090:1108	Expression analysis	1090:1108	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	0	30	theme	genes	33:37	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of AGPase genes	0:37	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	11	31	dep	seed	1631:1634	arg1	lotus					1625:1629	lotus seed	1625:1634	lotus seed	1625:1634	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	6	32	theme	AGPase	848:853	arg1	genes					855:859	Seven putative AGPase genes	833:859	Seven putative AGPase genes	833:859	Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.)					
33023477	1	33	theme	functional	286:295	arg1	food					297:300	hypoglycemic and low-glycemic index functional food	250:300	hypoglycemic and low-glycemic index functional food	250:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	8	34	theme	AGPase	1076:1081	arg1	genes					1083:1087	AGPase genes	1076:1087	AGPase genes	1076:1087	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	9	35	theme	lotus	1124:1128	arg1	genes					1137:1141	lotus AGPase genes	1124:1141	lotus AGPase genes	1124:1141	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	2	36	theme	improving	314:322	arg1	content					331:337	improving starch content	314:337	improving starch content	314:337	Currently, improving starch content is one of the major goals for seed-lotus breeding.					
33023477	1	37	theme	seed	202:205	arg1	property					219:226	lotus seed a promising property	196:226	lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food	196:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	6	38	dep	identified	866:875	arg1	Gaertn					915:920	Gaertn	915:920	Gaertn	915:920	Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.)					
33023477	4	39	with	seed	613:616	arg1	starch					642:647	starch	642:647	starch	642:647	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	10	40	theme	sucrose	1341:1347	arg1	metabolism					1349:1358	sucrose metabolism	1341:1358	sucrose metabolism	1341:1358	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	1	41	theme	promising	209:217	arg1	property					219:226	lotus seed a promising property	196:226	lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food	196:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	9	42	contain	have	1143:1146	arg1	genes					1137:1141	lotus AGPase genes	1124:1141	lotus AGPase genes	1124:1141	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	9	42	contain	have	1143:1146	arg2	patterns					1167:1174	varying expression patterns	1148:1174	varying expression patterns	1148:1174	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	9	43	theme	varying	1148:1154	arg1	patterns					1167:1174	varying expression patterns	1148:1174	varying expression patterns	1148:1174	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	6	44	theme	lotus	884:888	arg1	genome					890:895	the lotus genome	880:895	the lotus genome	880:895	Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.)					
33023477	12	45	theme	lotus	1758:1762	arg1	varieties					1764:1772	new lotus varieties	1754:1772	new lotus varieties with high-starch content	1754:1797	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	1	46	contain	contains	144:151	arg1	Starch					119:124	BACKGROUND Starch	108:124	BACKGROUND Starch in the lotus seed	108:142	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	1	46	contain	contains	144:151	arg2	proportion					160:169	a high proportion	153:169	a high proportion	153:169	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	10	47	theme	pathway	1360:1366	arg1	genes					1376:1380	pathway related genes	1360:1380	pathway related genes	1360:1380	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	10	48	dep	starch	1330:1335	arg1	genes					1376:1380	pathway related genes	1360:1380	pathway related genes	1360:1380	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	1	49	theme	high	155:158	arg1	proportion					160:169	a high proportion	153:169	a high proportion	153:169	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	8	50	theme	pressure	980:987	arg1	analysis					989:996	Selective pressure analysis	970:996	Selective pressure analysis	970:996	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	5	51	theme	transcriptome	683:695	arg1	analysis					697:704	Comparative transcriptome analysis	671:704	Comparative transcriptome analysis	671:704	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	5	52	theme	starch	817:822	arg1	content					824:830	significant different starch content	795:830	significant different starch content	795:830	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	5	53	dep	varieties	768:776	arg1	JX					786:787	JX	786:787	JX	786:787	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	5	53	dep	varieties	768:776	arg1	CA					779:780	CA	779:780	CA	779:780	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	5	53	dep	varieties	768:776	arg1	varieties					768:776	two varieties	764:776	two varieties (CA and JX) with significant different starch content	764:830	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	4	54	theme	seed	613:616	arg1	compositions					591:602	the nutritional compositions	575:602	the nutritional compositions of lotus seed among 30 varieties with starch	575:647	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	4	54	theme	seed	613:616	arg1	component					660:668	a major component	652:668	a major component	652:668	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	8	55	from	force	1050:1054	arg1	evolution					1063:1071	the evolution	1059:1071	the evolution of AGPase genes	1059:1087	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	5	56	theme	AGPase	718:723	arg1	genes					725:729	AGPase genes	718:729	AGPase genes	718:729	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	4	57	dep	RESULTS	555:561	arg1	describe					566:573	describe	566:573	describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component	566:668	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	1	58	theme	BACKGROUND	108:117	arg1	Starch					119:124	BACKGROUND Starch	108:124	BACKGROUND Starch in the lotus seed	108:142	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	10	59	from	content	1462:1468	arg1	seed					1479:1482	seed	1479:1482	seed	1479:1482	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	5	60	with	varieties	768:776	arg1	content					824:830	significant different starch content	795:830	significant different starch content	795:830	Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content.					
33023477	1	61	theme	lotus	133:137	arg1	seed					139:142	the lotus seed	129:142	the lotus seed	129:142	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	12	62	theme	AGPase	1698:1703	arg1	genes					1705:1709	lotus AGPase genes	1692:1709	lotus AGPase genes	1692:1709	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	4	63	theme	major	654:658	arg1	compositions					591:602	the nutritional compositions	575:602	the nutritional compositions of lotus seed among 30 varieties with starch	575:647	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	4	63	theme	major	654:658	arg1	component					660:668	a major component	652:668	a major component	652:668	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	11	64	theme	AGPase	1503:1508	arg1	genes					1584:1588	the key genes	1576:1588	the key genes involved in starch biosynthesis in lotus seed	1576:1634	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	11	64	theme	AGPase	1503:1508	arg1	genes					1510:1514	CONCLUSIONS Seven AGPase genes	1485:1514	CONCLUSIONS Seven AGPase genes	1485:1514	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	8	65	theme	purifying	1013:1021	arg1	selection					1023:1031	purifying selection	1013:1031	purifying selection	1013:1031	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	8	65	theme	purifying	1013:1021	arg1	force					1050:1054	a vital force	1042:1054	a vital force in the evolution of AGPase genes	1042:1087	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	10	66	theme	increased	1425:1433	arg1	activity					1442:1449	increased AGPase activity	1425:1449	increased AGPase activity	1425:1449	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	11	67	theme	CONCLUSIONS	1485:1495	arg1	genes					1584:1588	the key genes	1576:1588	the key genes involved in starch biosynthesis in lotus seed	1576:1634	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	11	67	theme	CONCLUSIONS	1485:1495	arg1	genes					1510:1514	CONCLUSIONS Seven AGPase genes	1485:1514	CONCLUSIONS Seven AGPase genes	1485:1514	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	0	68	theme	lotus	96:100	arg1	seed					102:105	lotus seed	96:105	lotus seed	96:105	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	9	69	theme	AGPase	1130:1135	arg1	genes					1137:1141	lotus AGPase genes	1124:1141	lotus AGPase genes	1124:1141	Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome.					
33023477	10	70	theme	starch	1455:1460	arg1	content					1462:1468	starch content	1455:1468	starch content	1455:1468	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	4	71	theme	nutritional	579:589	arg1	compositions					591:602	the nutritional compositions	575:602	the nutritional compositions of lotus seed among 30 varieties with starch	575:647	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	4	71	theme	nutritional	579:589	arg1	component					660:668	a major component	652:668	a major component	652:668	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	2	72	theme	major	353:357	arg1	goals					359:363	the major goals	349:363	the major goals for seed-lotus breeding	349:387	Currently, improving starch content is one of the major goals for seed-lotus breeding.					
33023477	0	73	theme	AGPase	26:31	arg1	genes					33:37	AGPase genes	26:37	AGPase genes	26:37	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	1	74	from	Starch	119:124	arg1	seed					139:142	the lotus seed	129:142	the lotus seed	129:142	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	1	75	theme	index	280:284	arg1	food					297:300	hypoglycemic and low-glycemic index functional food	250:300	hypoglycemic and low-glycemic index functional food	250:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	11	76	from	biosynthesis	1609:1620	arg1	seed					1631:1634	seed	1631:1634	seed	1631:1634	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	3	77	theme	essential	438:446	arg1	role					448:451	an essential role	435:451	an essential role	435:451	ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus.					
33023477	0	78	theme	potential	54:62	arg1	roles					64:68	their potential roles	48:68	their potential roles in starch biosynthesis in lotus seed	48:105	Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.					
33023477	1	79	theme	food	297:300	arg1	development					235:245	the development	231:245	the development of hypoglycemic and low-glycemic index functional food	231:300	BACKGROUND Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food.					
33023477	8	80	theme	genes	1083:1087	arg1	evolution					1063:1071	the evolution	1059:1071	the evolution of AGPase genes	1059:1087	Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes.					
33023477	6	81	theme	putative	839:846	arg1	genes					855:859	Seven putative AGPase genes	833:859	Seven putative AGPase genes	833:859	Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.)					
33023477	2	82	theme	starch	324:329	arg1	content					331:337	improving starch content	314:337	improving starch content	314:337	Currently, improving starch content is one of the major goals for seed-lotus breeding.					
33023477	10	83	theme	metabolism	1349:1358	arg1	number					1320:1325	a number	1318:1325	a number of starch and sucrose metabolism pathway related genes	1318:1380	NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed.					
33023477	3	84	theme	starch	467:472	arg1	biosynthesis					474:485	starch biosynthesis	467:485	starch biosynthesis in plants	467:495	ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus.					
33023477	12	85	with	varieties	1764:1772	arg1	content					1791:1797	high-starch content	1779:1797	high-starch content	1779:1797	These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.					
33023477	4	86	theme	lotus	607:611	arg1	seed					613:616	lotus seed	607:616	lotus seed among 30 varieties with starch	607:647	RESULTS We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component.					
33023477	11	87	theme	key	1580:1582	arg1	genes					1584:1588	the key genes	1576:1588	the key genes involved in starch biosynthesis in lotus seed	1576:1634	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
33023477	11	87	theme	key	1580:1582	arg1	genes					1510:1514	CONCLUSIONS Seven AGPase genes	1485:1514	CONCLUSIONS Seven AGPase genes	1485:1514	CONCLUSIONS Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed.					
32829858	1	0	theme	critical	164:171	arg1	problems					173:180	the most common but critical problems	144:180	the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria	144:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	1	1	theme	bacteria	255:262	arg1	resistance					241:250	the general antibiotic resistance	218:250	the general antibiotic resistance of bacteria	218:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	4	2	theme	Scanning	572:579	arg1	microscopy					590:599	Scanning electron microscopy	572:599	Scanning electron microscopy	572:599	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	1	3	theme	problems	173:180	arg1	one					137:139	one	137:139	one	137:139	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	1	3	theme	problems	173:180	arg1	infection					124:132	The bacterial infection	110:132	The bacterial infection	110:132	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	1	3	theme	problems	173:180	arg1	problems					173:180	the most common but critical problems	144:180	the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria	144:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	4	4	theme	freeze-dried	659:670	arg1	aerogel					681:687	freeze-dried chitosan aerogel	659:687	freeze-dried chitosan aerogel	659:687	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	7	5	contain	have	1133:1136	arg2	potential					1144:1152	great potential	1138:1152	great potential	1138:1152	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	7	5	contain	have	1133:1136	arg1	CS/NMNSs					1124:1131	the CS/NMNSs	1120:1131	the CS/NMNSs	1120:1131	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	1	6	from	problems	173:180	arg1	process					203:209	the wound healing process	185:209	the wound healing process due to the general antibiotic resistance of bacteria	185:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	7	7	from	agent	1200:1204	arg1	application					1216:1226	future application	1209:1226	future application	1209:1226	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	3	8	dep	embedded	405:412	arg1	nanosheets					456:465	amino-functionalized molybdenum disulfide nanosheets	414:465	amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs)	414:491	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	3	9	theme	physical	553:560	arg1	assembly					562:569	physical assembly	553:569	physical assembly	553:569	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	4	10	dep	microscopy	590:599	arg1	characterizations					601:617	characterizations	601:617	characterizations	601:617	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	3	11	theme	amino	530:534	arg1	modification					536:547	amino modification	530:547	amino modification	530:547	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	6	12	theme	elimination	994:1004	arg1	capacity					1006:1013	efficient bacterial elimination capacity	974:1013	efficient bacterial elimination capacity	974:1013	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	4	13	theme	sponge-like	724:734	arg1	structure					736:744	extremely regular sponge-like structure	706:744	extremely regular sponge-like structure	706:744	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	4	14	theme	favorable	766:774	arg1	property					785:792	favorable swelling property	766:792	favorable swelling property	766:792	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	5	15	theme	ideal	828:832	arg1	aerogel					802:808	The CS aerogel	795:808	The CS aerogel	795:808	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	5	15	theme	ideal	828:832	arg1	agent					855:859	an ideal bacterial adsorption agent	825:859	an ideal bacterial adsorption agent ascribed to its inherent positive charge	825:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	6	16	theme	bacterial	984:992	arg1	capacity					1006:1013	efficient bacterial elimination capacity	974:1013	efficient bacterial elimination capacity	974:1013	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	4	17	theme	chitosan	672:679	arg1	aerogel					681:687	freeze-dried chitosan aerogel	659:687	freeze-dried chitosan aerogel	659:687	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	3	18	theme	embedded	405:412	arg1	aerogel					397:403	a chitosan-based aerogel	380:403	a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs)	380:491	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	6	19	theme	aerogel	1047:1053	arg1	ability					1027:1033	capture ability	1019:1033	capture ability of chitosan aerogel	1019:1053	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	0	20	theme	bifunctional	15:26	arg1	aerogel					38:44	Chitosan-based bifunctional composite aerogel	0:44	Chitosan-based bifunctional composite aerogel	0:44	Chitosan-based bifunctional composite aerogel combining absorption and phototherapy for bacteria elimination.					
32829858	6	21	theme	efficient	974:982	arg1	capacity					1006:1013	efficient bacterial elimination capacity	974:1013	efficient bacterial elimination capacity	974:1013	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	7	22	theme	future	1209:1214	arg1	application					1216:1226	future application	1209:1226	future application	1209:1226	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	5	23	theme	bacterial	834:842	arg1	aerogel					802:808	The CS aerogel	795:808	The CS aerogel	795:808	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	5	23	theme	bacterial	834:842	arg1	agent					855:859	an ideal bacterial adsorption agent	825:859	an ideal bacterial adsorption agent ascribed to its inherent positive charge	825:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	1	24	theme	wound	189:193	arg1	healing					195:201	wound healing	189:201	the wound healing process due to the general antibiotic resistance of bacteria	185:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	0	25	theme	Chitosan-based	0:13	arg1	aerogel					38:44	Chitosan-based bifunctional composite aerogel	0:44	Chitosan-based bifunctional composite aerogel	0:44	Chitosan-based bifunctional composite aerogel combining absorption and phototherapy for bacteria elimination.					
32829858	3	26	dep	nanosheets	456:465	arg1	abbreviated					468:478	abbreviated	468:478	abbreviated to CS/NMNSs	468:490	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	1	27	theme	bacterial	114:122	arg1	one					137:139	one	137:139	one	137:139	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	1	27	theme	bacterial	114:122	arg1	infection					124:132	The bacterial infection	110:132	The bacterial infection	110:132	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	1	27	theme	bacterial	114:122	arg1	problems					173:180	the most common but critical problems	144:180	the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria	144:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	1	28	theme	healing	195:201	arg1	process					203:209	the wound healing process	185:209	the wound healing process due to the general antibiotic resistance of bacteria	185:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	4	29	theme	swelling	623:630	arg1	experiments					632:642	swelling experiments	623:642	swelling experiments	623:642	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	5	30	theme	CS	799:800	arg1	aerogel					802:808	The CS aerogel	795:808	The CS aerogel	795:808	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	5	30	theme	CS	799:800	arg1	agent					855:859	an ideal bacterial adsorption agent	825:859	an ideal bacterial adsorption agent ascribed to its inherent positive charge	825:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	5	31	theme	inherent	877:884	arg1	charge					895:900	its inherent positive charge	873:900	its inherent positive charge	873:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	0	32	theme	composite	28:36	arg1	aerogel					38:44	Chitosan-based bifunctional composite aerogel	0:44	Chitosan-based bifunctional composite aerogel	0:44	Chitosan-based bifunctional composite aerogel combining absorption and phototherapy for bacteria elimination.					
32829858	6	33	theme	induced	1073:1079	arg1	sterilization					1094:1106	infrared induced photothermal sterilization	1064:1106	infrared induced photothermal sterilization	1064:1106	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	1	34	theme	due	211:213	arg1	process					203:209	the wound healing process	185:209	the wound healing process due to the general antibiotic resistance of bacteria	185:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	3	35	theme	disulfide	446:454	arg1	nanosheets					456:465	amino-functionalized molybdenum disulfide nanosheets	414:465	amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs)	414:491	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	6	36	theme	infrared	1064:1071	arg1	sterilization					1094:1106	infrared induced photothermal sterilization	1064:1106	infrared induced photothermal sterilization	1064:1106	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	7	37	theme	photothermal	1173:1184	arg1	agent					1200:1204	a photothermal antibacterial agent	1171:1204	a photothermal antibacterial agent in future application	1171:1226	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	4	38	theme	high	747:750	arg1	porosity					752:759	high porosity	747:759	high porosity	747:759	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	7	39	theme	antibacterial	1186:1198	arg1	agent					1200:1204	a photothermal antibacterial agent	1171:1204	a photothermal antibacterial agent in future application	1171:1226	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	7	40	theme	great	1138:1142	arg1	potential					1144:1152	great potential	1138:1152	great potential	1138:1152	Therefore, the CS/NMNSs have great potential in developing as a photothermal antibacterial agent in future application.					
32829858	3	41	theme	amino-functionalized	414:433	arg1	nanosheets					456:465	amino-functionalized molybdenum disulfide nanosheets	414:465	amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs)	414:491	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	4	42	theme	electron	581:588	arg1	microscopy					590:599	Scanning electron microscopy	572:599	Scanning electron microscopy	572:599	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	5	43	theme	positive	886:893	arg1	charge					895:900	its inherent positive charge	873:900	its inherent positive charge	873:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	6	44	theme	photothermal	1081:1092	arg1	sterilization					1094:1106	infrared induced photothermal sterilization	1064:1106	infrared induced photothermal sterilization	1064:1106	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	2	45	theme	sterilization	348:360	arg1	strategy					362:369	an advanced and efficient sterilization strategy	322:369	an advanced and efficient sterilization strategy	322:369	Hence it is increasingly necessary and urgent to develop an advanced and efficient sterilization strategy.					
32829858	3	46	theme	molybdenum	435:444	arg1	nanosheets					456:465	amino-functionalized molybdenum disulfide nanosheets	414:465	amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs)	414:491	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	5	47	used	used	817:820	arg2	aerogel					802:808	The CS aerogel	795:808	The CS aerogel	795:808	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	5	47	used	used	817:820	arg2	agent					855:859	an ideal bacterial adsorption agent	825:859	an ideal bacterial adsorption agent ascribed to its inherent positive charge	825:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	6	48	theme	capture	1019:1025	arg1	ability					1027:1033	capture ability	1019:1033	capture ability of chitosan aerogel	1019:1053	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	2	49	theme	efficient	338:346	arg1	strategy					362:369	an advanced and efficient sterilization strategy	322:369	an advanced and efficient sterilization strategy	322:369	Hence it is increasingly necessary and urgent to develop an advanced and efficient sterilization strategy.					
32829858	6	50	theme	chitosan	1038:1045	arg1	aerogel					1047:1053	chitosan aerogel	1038:1053	chitosan aerogel	1038:1053	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	6	51	theme	studies	931:937	arg1	result					907:912	The result	903:912	The result of antibacterial studies	903:937	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	1	52	theme	general	222:228	arg1	resistance					241:250	the general antibiotic resistance	218:250	the general antibiotic resistance of bacteria	218:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	6	53	theme	antibacterial	917:929	arg1	studies					931:937	antibacterial studies	917:937	antibacterial studies	917:937	The result of antibacterial studies showed that the CS/NMNSs exhibited efficient bacterial elimination capacity via capture ability of chitosan aerogel and near infrared induced photothermal sterilization.					
32829858	2	54	theme	advanced	325:332	arg1	strategy					362:369	an advanced and efficient sterilization strategy	322:369	an advanced and efficient sterilization strategy	322:369	Hence it is increasingly necessary and urgent to develop an advanced and efficient sterilization strategy.					
32829858	1	55	theme	antibiotic	230:239	arg1	resistance					241:250	the general antibiotic resistance	218:250	the general antibiotic resistance of bacteria	218:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	0	56	theme	bacteria	88:95	arg1	elimination					97:107	bacteria elimination	88:107	bacteria elimination	88:107	Chitosan-based bifunctional composite aerogel combining absorption and phototherapy for bacteria elimination.					
32829858	1	57	theme	common	153:158	arg1	problems					173:180	the most common but critical problems	144:180	the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria	144:262	The bacterial infection is one of the most common but critical problems in the wound healing process due to the general antibiotic resistance of bacteria.					
32829858	4	58	theme	regular	716:722	arg1	structure					736:744	extremely regular sponge-like structure	706:744	extremely regular sponge-like structure	706:744	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
32829858	5	59	theme	adsorption	844:853	arg1	aerogel					802:808	The CS aerogel	795:808	The CS aerogel	795:808	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	5	59	theme	adsorption	844:853	arg1	agent					855:859	an ideal bacterial adsorption agent	825:859	an ideal bacterial adsorption agent ascribed to its inherent positive charge	825:900	The CS aerogel can be used as an ideal bacterial adsorption agent ascribed to its inherent positive charge.					
32829858	3	60	theme	chitosan-based	382:395	arg1	aerogel					397:403	a chitosan-based aerogel	380:403	a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs)	380:491	Herein, a chitosan-based aerogel embedded amino-functionalized molybdenum disulfide nanosheets (abbreviated to CS/NMNSs) was successfully constructed through amino modification and physical assembly.					
32829858	4	61	theme	swelling	776:783	arg1	property					785:792	favorable swelling property	766:792	favorable swelling property	766:792	Scanning electron microscopy characterizations and swelling experiments indicated that freeze-dried chitosan aerogel is provided with extremely regular sponge-like structure, high porosity, and favorable swelling property.					
34050560	4	0	theme	metronidazole	977:989	arg1	0.5 g/L					924:930	0.5 g/L	924:930	0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water	924:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	8	1	theme	Muc2	1614:1617	arg1	synthesis					1601:1609	the synthesis	1597:1609	the synthesis of Muc2	1597:1617	However, it exhibited no significant effect on the synthesis of Muc2.					
34050560	6	2	theme	composition	1236:1246	arg1	independent					1213:1223	independent	1213:1223	independent	1213:1223	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	2	3	theme	high-protein	331:342	arg1	diets					344:348	high-protein diets	331:348	high-protein diets from different dietary proteins (casein, whey protein, soy protein)	331:416	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	5	4	theme	mucus	1136:1140	arg1	layer					1142:1146	mucus layer	1136:1146	mucus layer	1136:1146	Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.					
34050560	4	5	theme	dietary	800:806	arg1	interventions					808:820	different dietary interventions	790:820	different dietary interventions	790:820	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	7	6	theme	thetaiotaomicron	1409:1424	arg1	composition					1334:1344	the composition	1330:1344	the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria	1330:1459	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	4	7	theme	ampicillin	995:1004	arg1	0.5 g/L					924:930	0.5 g/L	924:930	0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water	924:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	10	8	theme	CONCLUSION	1773:1782	arg1	diet					1797:1800	CONCLUSION High-protein diet	1773:1800	CONCLUSION High-protein diet	1773:1800	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	4	9	theme	mucus	758:762	arg1	layer					764:768	colonic mucus layer	750:768	colonic mucus layer	750:768	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	2	10	from	effect	321:326	arg1	microbiota					460:469	microbiota	460:469	microbiota	460:469	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	10	from	effect	321:326	arg1	colitis					434:440	experimental colitis	421:440	experimental colitis	421:440	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	10	from	effect	321:326	arg1	function					486:493	function	486:493	function	486:493	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	10	from	effect	321:326	arg1	proteins					373:380	different dietary proteins	355:380	different dietary proteins (casein, whey protein, soy protein)	355:416	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	10	from	effect	321:326	arg1	structure					472:480	structure	472:480	structure	472:480	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	7	11	theme	mucin-degrading	1436:1450	arg1	bacteria					1452:1459	total mucin-degrading bacteria	1430:1459	total mucin-degrading bacteria	1430:1459	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	9	12	theme	bacteria	1690:1697	arg1	abundance					1661:1669	the abundance	1657:1669	the abundance of mucin-degrading bacteria and sialidase concentration	1657:1725	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	5	13	theme	sialidase	1099:1107	arg1	synthesis					1064:1072	the synthesis	1060:1072	the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer	1060:1146	Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.					
34050560	6	14	theme	acute	1187:1191	arg1	colitis					1205:1211	acute DSS-induced colitis	1187:1211	acute DSS-induced colitis independent of protein composition	1187:1246	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	10	15	theme	microbial	1809:1817	arg1	composition					1819:1829	microbial composition	1809:1829	microbial composition	1809:1829	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	1	16	theme	inflammatory	190:201	arg1	diseases					209:216	inflammatory bowel diseases	190:216	inflammatory bowel diseases (IBDs)	190:223	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	1	16	theme	inflammatory	190:201	arg1	IBDs					219:222	IBDs	219:222	IBDs	219:222	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	7	17	from	bacteria	1452:1459	arg1	layer					1379:1383	the colonic mucus layer	1361:1383	the colonic mucus layer	1361:1383	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	9	18	theme	sialidase	1703:1711	arg1	concentration					1713:1725	sialidase concentration	1703:1725	sialidase concentration	1703:1725	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	7	19	theme	colonic	1365:1371	arg1	layer					1379:1383	the colonic mucus layer	1361:1383	the colonic mucus layer	1361:1383	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	3	20	theme	Female	527:532	arg1	mice					541:544	METHODS Female BALB/c mice	519:544	METHODS Female BALB/c mice	519:544	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	2	21	theme	mucus	506:510	arg1	layer					512:516	colonic mucus layer	498:516	colonic mucus layer	498:516	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	22	from	impact	450:455	arg1	microbiota					460:469	microbiota	460:469	microbiota	460:469	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	22	from	impact	450:455	arg1	colitis					434:440	experimental colitis	421:440	experimental colitis	421:440	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	22	from	impact	450:455	arg1	function					486:493	function	486:493	function	486:493	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	22	from	impact	450:455	arg1	proteins					373:380	different dietary proteins	355:380	different dietary proteins (casein, whey protein, soy protein)	355:416	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	22	from	impact	450:455	arg1	structure					472:480	structure	472:480	structure	472:480	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	3	23	theme	standard	558:565	arg1	diet					567:570	standard diet	558:570	standard diet	558:570	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	6	24	theme	High-protein	1157:1168	arg1	diets					1170:1174	High-protein diets	1157:1174	RESULTS High-protein diets	1149:1174	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	4	25	dep	intervention	910:921	arg1	0.5 g/L					924:930	0.5 g/L	924:930	0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water	924:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	1	26	theme	BACKGROUND	122:131	arg1	diet					162:165	BACKGROUND AND AIM Westernized high-fat diet	122:165	diet	162:165	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	1	27	theme	AIM	137:139	arg1	diet					162:165	BACKGROUND AND AIM Westernized high-fat diet	122:165	diet	162:165	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	7	28	from	layer	1379:1383	arg1	composition					1334:1344	the composition	1330:1344	the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria	1330:1459	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	4	29	theme	intervention	910:921	arg1	effect					874:879	the reversal effect	861:879	the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water)	861:1023	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	4	30	theme	sulfate	968:974	arg1	ampicillin					995:1004	ampicillin	995:1004	ampicillin	995:1004	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	4	30	theme	sulfate	968:974	arg1	metronidazole					977:989	metronidazole	977:989	metronidazole	977:989	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	4	30	theme	sulfate	968:974	arg1	1 g/L					950:954	1 g/L	950:954	1 g/L	950:954	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	4	30	theme	sulfate	968:974	arg1	vancomycin					935:944	vancomycin	935:944	vancomycin	935:944	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	1	31	theme	high-fat	153:160	arg1	diet					162:165	BACKGROUND AND AIM Westernized high-fat diet	122:165	diet	162:165	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	0	32	theme	mucus	73:77	arg1	layer					79:83	thinner mucus layer	65:83	thinner mucus layer	65:83	Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis.					
34050560	7	33	from	thetaiotaomicron	1409:1424	arg1	layer					1379:1383	the colonic mucus layer	1361:1383	the colonic mucus layer	1361:1383	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	4	34	theme	effect	874:879	arg1	identification					843:856	the identification	839:856	the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water)	839:1023	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	5	35	theme	Further	1026:1032	arg1	analysis					1034:1041	Further analysis	1026:1041	Further analysis	1026:1041	Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.					
34050560	4	36	theme	broad-spectrum	884:897	arg1	intervention					910:921	broad-spectrum antibiotic intervention	884:921	broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water)	884:1023	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	0	37	theme	experimental	100:111	arg1	colitis					113:119	experimental colitis	100:119	experimental colitis	100:119	Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis.					
34050560	4	38	theme	neomycin	959:966	arg1	sulfate					968:974	neomycin sulfate	959:974	neomycin sulfate	959:974	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	9	39	theme	layer	1766:1770	arg1	thickness					1747:1755	the thickness	1743:1755	the thickness of mucus layer	1743:1770	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	1	40	theme	role	269:272	arg1	understanding					248:260	insufficient understanding	235:260	insufficient understanding of the role of high-protein diet	235:293	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	4	41	from	susceptibility	662:675	arg1	mice					728:731	mice	728:731	mice	728:731	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	1	42	theme	high-protein	277:288	arg1	diet					290:293	high-protein diet	277:293	high-protein diet	277:293	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	4	43	theme	vancomycin	935:944	arg1	0.5 g/L					924:930	0.5 g/L	924:930	0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water	924:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	2	44	theme	whey	391:394	arg1	casein					383:388	casein	383:388	casein	383:388	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	44	theme	whey	391:394	arg1	protein					396:402	whey protein	391:402	whey protein	391:402	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	0	45	theme	high	38:41	arg1	diet					51:54	high protein diet	38:54	high protein diet	38:54	Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis.					
34050560	5	46	theme	mucin	1077:1081	arg1	synthesis					1064:1072	the synthesis	1060:1072	the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer	1060:1146	Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.					
34050560	2	47	from	proteins	373:380	arg1	effect					321:326	the effect	317:326	the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis	317:440	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	47	from	proteins	373:380	arg1	diets					344:348	high-protein diets	331:348	high-protein diets from different dietary proteins (casein, whey protein, soy protein)	331:416	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	47	from	proteins	373:380	arg1	impact					450:455	its impact	446:455	its impact on microbiota, structure and function of colonic mucus layer	446:516	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	4	48	theme	1 g/L	950:954	arg1	0.5 g/L					924:930	0.5 g/L	924:930	0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water	924:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	5	49	theme	microbiota	1084:1093	arg1	synthesis					1064:1072	the synthesis	1060:1072	the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer	1060:1146	Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.					
34050560	9	50	theme	mucin-degrading	1674:1688	arg1	bacteria					1690:1697	mucin-degrading bacteria	1674:1697	mucin-degrading bacteria	1674:1697	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	10	51	theme	mucus	1856:1860	arg1	layer					1862:1866	colonic mucus layer	1848:1866	colonic mucus layer	1848:1866	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	10	52	theme	colitis	1917:1923	arg1	aggravation					1884:1894	the aggravation	1880:1894	the aggravation of acute DSS-induced colitis	1880:1923	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	2	53	dep	proteins	373:380	arg1	casein					383:388	casein	383:388	casein	383:388	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	53	dep	proteins	373:380	arg1	protein					396:402	whey protein	391:402	whey protein	391:402	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	53	dep	proteins	373:380	arg1	protein					409:415	soy protein	405:415	soy protein	405:415	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	4	54	from	0.5 g/L	924:930	arg1	water					1018:1022	drinking water	1009:1022	drinking water	1009:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	7	55	theme	sialidase	1484:1492	arg1	concentration					1494:1506	sialidase concentration	1484:1506	sialidase concentration	1484:1506	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	3	56	theme	whey	602:605	arg1	diet					615:618	high whey protein diet	597:618	high whey protein diet	597:618	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	2	57	theme	different	355:363	arg1	proteins					373:380	different dietary proteins	355:380	different dietary proteins (casein, whey protein, soy protein)	355:416	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	9	58	theme	Broad-spectrum	1620:1633	arg1	antibiotics					1635:1645	Broad-spectrum antibiotics	1620:1645	Broad-spectrum antibiotics	1620:1645	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	3	59	theme	protein	632:638	arg1	diet					640:643	high soy protein diet	623:643	high soy protein diet	623:643	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	2	60	theme	diets	344:348	arg1	effect					321:326	the effect	317:326	the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis	317:440	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	60	theme	diets	344:348	arg1	impact					450:455	its impact	446:455	its impact on microbiota, structure and function of colonic mucus layer	446:516	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	1	61	theme	insufficient	235:246	arg1	understanding					248:260	insufficient understanding	235:260	insufficient understanding of the role of high-protein diet	235:293	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	3	62	theme	high	623:626	arg1	diet					640:643	high soy protein diet	623:643	high soy protein diet	623:643	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	6	63	theme	protein	1228:1234	arg1	composition					1236:1246	protein composition	1228:1246	protein composition	1228:1246	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	10	64	theme	High-protein	1784:1795	arg1	diet					1797:1800	CONCLUSION High-protein diet	1773:1800	CONCLUSION High-protein diet	1773:1800	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	5	65	theme	layer	1142:1146	arg1	degradation					1121:1131	degradation	1121:1131	degradation of mucus layer	1121:1146	Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.					
34050560	7	66	theme	bacteria	1452:1459	arg1	composition					1334:1344	the composition	1330:1344	the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria	1330:1459	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	4	67	theme	different	790:798	arg1	interventions					808:820	different dietary interventions	790:820	different dietary interventions	790:820	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	6	68	theme	independent	1213:1223	arg1	colitis					1205:1211	acute DSS-induced colitis	1187:1211	acute DSS-induced colitis independent of protein composition	1187:1246	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	7	69	theme	total	1430:1434	arg1	bacteria					1452:1459	total mucin-degrading bacteria	1430:1459	total mucin-degrading bacteria	1430:1459	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	4	70	theme	colonic	750:756	arg1	layer					764:768	colonic mucus layer	750:768	colonic mucus layer	750:768	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	7	71	theme	Bacteroides	1397:1407	arg1	thetaiotaomicron					1409:1424	Bacteroides thetaiotaomicron	1397:1424	Bacteroides thetaiotaomicron	1397:1424	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	6	72	theme	DSS-induced	1193:1203	arg1	colitis					1205:1211	acute DSS-induced colitis	1187:1211	acute DSS-induced colitis independent of protein composition	1187:1246	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	3	73	theme	BALB/c	534:539	arg1	mice					541:544	METHODS Female BALB/c mice	519:544	METHODS Female BALB/c mice	519:544	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	4	74	theme	layer	764:768	arg1	colitis					717:723	dextran sulfate sodium (DSS)-induced colitis	680:723	dextran sulfate sodium (DSS)-induced colitis in mice	680:731	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	4	74	theme	layer	764:768	arg1	thickness					737:745	thickness	737:745	thickness of colonic mucus layer	737:768	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	3	75	theme	METHODS	519:525	arg1	mice					541:544	METHODS Female BALB/c mice	519:544	METHODS Female BALB/c mice	519:544	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	1	76	theme	bowel	203:207	arg1	diseases					209:216	inflammatory bowel diseases	190:216	inflammatory bowel diseases (IBDs)	190:223	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	1	76	theme	bowel	203:207	arg1	IBDs					219:222	IBDs	219:222	IBDs	219:222	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	2	77	theme	layer	512:516	arg1	effect					321:326	the effect	317:326	the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis	317:440	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	77	theme	layer	512:516	arg1	impact					450:455	its impact	446:455	its impact on microbiota, structure and function of colonic mucus layer	446:516	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	7	78	theme	mucus	1373:1377	arg1	layer					1379:1383	the colonic mucus layer	1361:1383	the colonic mucus layer	1361:1383	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	10	79	theme	colonic	1848:1854	arg1	layer					1862:1866	colonic mucus layer	1848:1866	colonic mucus layer	1848:1866	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	2	80	theme	colonic	498:504	arg1	layer					512:516	colonic mucus layer	498:516	colonic mucus layer	498:516	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	4	81	from	thickness	737:745	arg1	mice					728:731	mice	728:731	mice	728:731	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	7	82	theme	bacteria	1349:1356	arg1	composition					1334:1344	the composition	1330:1344	the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria	1330:1459	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	6	83	theme	broad-spectrum	1255:1268	arg1	antibiotics					1270:1280	broad-spectrum antibiotics	1255:1280	broad-spectrum antibiotics	1255:1280	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	4	84	theme	-induced	708:715	arg1	colitis					717:723	dextran sulfate sodium (DSS)-induced colitis	680:723	dextran sulfate sodium (DSS)-induced colitis in mice	680:731	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	3	85	theme	high-casein	573:583	arg1	HCD					591:593	HCD	591:593	HCD	591:593	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	3	85	theme	high-casein	573:583	arg1	diet					585:588	high-casein diet	573:588	high-casein diet (HCD)	573:594	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	4	86	from	mice	728:731	arg1	susceptibility					662:675	The susceptibility	658:675	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer	658:768	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	9	87	theme	mucus	1760:1764	arg1	layer					1766:1770	mucus layer	1760:1770	mucus layer	1760:1770	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	0	88	theme	thinner	65:71	arg1	layer					79:83	thinner mucus layer	65:83	thinner mucus layer	65:83	Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis.					
34050560	10	89	theme	layer	1862:1866	arg1	thickness					1835:1843	thickness	1835:1843	thickness	1835:1843	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	10	89	theme	layer	1862:1866	arg1	composition					1819:1829	microbial composition	1809:1829	microbial composition	1809:1829	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	4	90	from	colitis	717:723	arg1	mice					728:731	mice	728:731	mice	728:731	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	1	91	theme	Westernized	141:151	arg1	diet					162:165	BACKGROUND AND AIM Westernized high-fat diet	122:165	diet	162:165	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	4	92	theme	antibiotic	899:908	arg1	intervention					910:921	broad-spectrum antibiotic intervention	884:921	broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water)	884:1023	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	6	93	dep	RESULTS	1149:1155	arg1	diets					1170:1174	High-protein diets	1157:1174	RESULTS High-protein diets	1149:1174	RESULTS High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect.					
34050560	9	94	theme	concentration	1713:1725	arg1	abundance					1661:1669	the abundance	1657:1669	the abundance of mucin-degrading bacteria and sialidase concentration	1657:1725	Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.					
34050560	10	95	theme	DSS-induced	1905:1915	arg1	colitis					1917:1923	acute DSS-induced colitis	1899:1923	acute DSS-induced colitis	1899:1923	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	7	96	theme	layer	1543:1547	arg1	thickness					1524:1532	the thickness	1520:1532	the thickness of mucus layer	1520:1547	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	4	97	theme	reversal	865:872	arg1	effect					874:879	the reversal effect	861:879	the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water)	861:1023	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	0	98	theme	mucin-degrading	10:24	arg1	bacteria					26:33	mucin-degrading bacteria	10:33	mucin-degrading bacteria	10:33	Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis.					
34050560	7	99	from	composition	1334:1344	arg1	layer					1379:1383	the colonic mucus layer	1361:1383	the colonic mucus layer	1361:1383	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	10	100	theme	acute	1899:1903	arg1	colitis					1917:1923	acute DSS-induced colitis	1899:1923	acute DSS-induced colitis	1899:1923	CONCLUSION High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.					
34050560	2	101	theme	dietary	365:371	arg1	proteins					373:380	different dietary proteins	355:380	different dietary proteins (casein, whey protein, soy protein)	355:416	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	7	102	from	bacteria	1349:1356	arg1	layer					1379:1383	the colonic mucus layer	1361:1383	the colonic mucus layer	1361:1383	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	2	103	theme	soy	405:407	arg1	casein					383:388	casein	383:388	casein	383:388	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	2	103	theme	soy	405:407	arg1	protein					409:415	soy protein	405:415	soy protein	405:415	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	0	104	theme	protein	43:49	arg1	diet					51:54	high protein diet	38:54	high protein diet	38:54	Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis.					
34050560	1	105	theme	diet	290:293	arg1	role					269:272	the role	265:272	the role of high-protein diet	265:293	BACKGROUND AND AIM Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet.					
34050560	7	106	theme	mucus	1537:1541	arg1	layer					1543:1547	mucus layer	1537:1547	mucus layer	1537:1547	HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer.					
34050560	2	107	theme	experimental	421:432	arg1	colitis					434:440	experimental colitis	421:440	experimental colitis	421:440	We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.					
34050560	8	108	theme	significant	1575:1585	arg1	effect					1587:1592	no significant effect	1572:1592	no significant effect	1572:1592	However, it exhibited no significant effect on the synthesis of Muc2.					
34050560	3	109	theme	protein	607:613	arg1	diet					615:618	high whey protein diet	597:618	high whey protein diet	597:618	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	4	110	theme	thickness	737:745	arg1	susceptibility					662:675	The susceptibility	658:675	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer	658:768	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	4	111	theme	drinking	1009:1016	arg1	water					1018:1022	drinking water	1009:1022	drinking water	1009:1022	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
34050560	3	112	theme	high	597:600	arg1	diet					615:618	high whey protein diet	597:618	high whey protein diet	597:618	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	3	113	theme	soy	628:630	arg1	diet					640:643	high soy protein diet	623:643	high soy protein diet	623:643	METHODS Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks.					
34050560	4	114	theme	colitis	717:723	arg1	susceptibility					662:675	The susceptibility	658:675	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer	658:768	The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water).					
32439439	5	0	theme	infrared	726:733	arg1	FT-IR					749:753	FT-IR	749:753	FT-IR	749:753	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	5	0	theme	infrared	726:733	arg1	spectroscopy					735:746	Fourier infrared spectroscopy	718:746	Fourier infrared spectroscopy (FT-IR)	718:754	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	4	1	theme	crosslinking	658:669	arg1	method					671:676	emulsion crosslinking method	649:676	emulsion crosslinking method	649:676	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	7	2	theme	crop	1193:1196	arg1	growth					1198:1203	crop growth	1193:1203	crop growth	1193:1203	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	4	3	theme	emulsion	649:656	arg1	method					671:676	emulsion crosslinking method	649:676	emulsion crosslinking method	649:676	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	4	4	theme	fulvic	523:528	arg1	FA					536:537	FA	536:537	FA	536:537	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	4	4	theme	fulvic	523:528	arg1	acid					530:533	fulvic acid	523:533	fulvic acid (FA)	523:538	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	7	5	theme	metal	1170:1174	arg1	ions					1176:1179	free heavy metal ions	1159:1179	free heavy metal ions	1159:1179	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	8	6	theme	utilization	1306:1316	arg1	rate					1318:1321	the utilization rate	1302:1321	the utilization rate of fertilizer	1302:1335	The load of fertilizer in the microsphere can effectively prolong the release cycle and improve the utilization rate of fertilizer.					
32439439	6	7	theme	thermogravimetric	953:969	arg1	TGA					981:983	TGA	981:983	TGA	981:983	The thermal stability of the microspheres was measured by thermogravimetric analysis (TGA).					
32439439	6	7	theme	thermogravimetric	953:969	arg1	analysis					971:978	thermogravimetric analysis	953:978	thermogravimetric analysis (TGA)	953:984	The thermal stability of the microspheres was measured by thermogravimetric analysis (TGA).					
32439439	7	8	theme	heavy	1164:1168	arg1	ions					1176:1179	free heavy metal ions	1159:1179	free heavy metal ions	1159:1179	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	1	9	from	elements	165:172	arg1	soil					177:180	soil	177:180	soil	177:180	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	8	10	theme	fertilizer	1326:1335	arg1	rate					1318:1321	the utilization rate	1302:1321	the utilization rate of fertilizer	1302:1335	The load of fertilizer in the microsphere can effectively prolong the release cycle and improve the utilization rate of fertilizer.					
32439439	4	11	theme	core-shell	620:629	arg1	structure					631:639	core-shell structure	620:639	core-shell structure	620:639	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	3	12	theme	Soil	334:337	arg1	conditioners					339:350	Soil conditioners	334:350	Soil conditioners prepared from sustainable macromolecules	334:391	Soil conditioners prepared from sustainable macromolecules can reduce or eliminate these negative effects.					
32439439	5	13	theme	GL/CS	872:876	arg1	materials					884:892	GL/CS shell materials	872:892	GL/CS shell materials	872:892	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	7	14	theme	water-retention	1118:1132	arg1	time					1134:1137	the water-retention time	1114:1137	the water-retention time of the soil	1114:1149	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	1	15	theme	Traditional	102:112	arg1	drugs					147:151	drugs	147:151	drugs	147:151	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	1	15	theme	Traditional	102:112	arg1	conditioners					119:130	Traditional soil conditioners	102:130	Traditional soil conditioners	102:130	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	4	16	theme	conditioner	590:600	arg1	microspheres					602:613	GL/CS/MAP-FA soil conditioner microspheres	572:613	GL/CS/MAP-FA soil conditioner microspheres with core-shell structure	572:639	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	1	17	theme	soil	114:117	arg1	drugs					147:151	drugs	147:151	drugs	147:151	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	1	17	theme	soil	114:117	arg1	conditioners					119:130	Traditional soil conditioners	102:130	Traditional soil conditioners	102:130	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	3	18	theme	negative	423:430	arg1	effects					432:438	these negative effects	417:438	these negative effects	417:438	Soil conditioners prepared from sustainable macromolecules can reduce or eliminate these negative effects.					
32439439	0	19	theme	Preparation	0:10	arg1	properties					28:37	Preparation and application properties	0:37	Preparation and application properties of sustainable	0:52	Preparation and application properties of sustainable gelatin/chitosan soil conditioner microspheres.					
32439439	6	20	theme	thermal	899:905	arg1	stability					907:915	The thermal stability	895:915	The thermal stability of the microspheres	895:935	The thermal stability of the microspheres was measured by thermogravimetric analysis (TGA).					
32439439	5	21	theme	good	844:847	arg1	performance					857:867	the good coating performance	840:867	the good coating performance of GL/CS shell materials	840:892	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	7	22	theme	soil	1146:1149	arg1	time					1134:1137	the water-retention time	1114:1137	the water-retention time of the soil	1114:1149	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	2	23	used	used	328:331	arg2	they					319:322	they	319:322	they	319:322	There are some negative effects such as low efficiency, hard to degrade and harmful to the environment when they are used.					
32439439	3	24	theme	sustainable	366:376	arg1	macromolecules					378:391	sustainable macromolecules	366:391	sustainable macromolecules	366:391	Soil conditioners prepared from sustainable macromolecules can reduce or eliminate these negative effects.					
32439439	1	25	used	used	139:142	arg2	conditioners					119:130	Traditional soil conditioners	102:130	Traditional soil conditioners	102:130	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	1	25	used	used	139:142	arg2	drugs					147:151	drugs	147:151	drugs	147:151	Traditional soil conditioners can be used as drugs to regulate elements in soil due to overuse or pollution.					
32439439	0	26	theme	sustainable	42:52	arg1	properties					28:37	Preparation and application properties	0:37	Preparation and application properties of sustainable	0:52	Preparation and application properties of sustainable gelatin/chitosan soil conditioner microspheres.					
32439439	6	27	theme	microspheres	924:935	arg1	stability					907:915	The thermal stability	895:915	The thermal stability of the microspheres	895:935	The thermal stability of the microspheres was measured by thermogravimetric analysis (TGA).					
32439439	5	28	theme	Scanning	679:686	arg1	SEM					709:711	SEM	709:711	SEM	709:711	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	5	28	theme	Scanning	679:686	arg1	microscopy					697:706	Scanning electron microscopy	679:706	Scanning electron microscopy (SEM)	679:712	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	7	29	theme	free	1159:1162	arg1	ions					1176:1179	free heavy metal ions	1159:1179	free heavy metal ions	1159:1179	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	4	30	theme	soil	585:588	arg1	microspheres					602:613	GL/CS/MAP-FA soil conditioner microspheres	572:613	GL/CS/MAP-FA soil conditioner microspheres with core-shell structure	572:639	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	4	31	theme	magnesium	484:492	arg1	MAP					514:516	MAP	514:516	MAP	514:516	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	4	31	theme	magnesium	484:492	arg1	phosphate					503:511	magnesium ammonium phosphate	484:511	magnesium ammonium phosphate (MAP)	484:517	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	5	32	theme	electron	688:695	arg1	SEM					709:711	SEM	709:711	SEM	709:711	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	5	32	theme	electron	688:695	arg1	microscopy					697:706	Scanning electron microscopy	679:706	Scanning electron microscopy (SEM)	679:712	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	4	33	with	microspheres	602:613	arg1	structure					631:639	core-shell structure	620:639	core-shell structure	620:639	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	4	34	theme	GL/CS/MAP-FA	572:583	arg1	microspheres					602:613	GL/CS/MAP-FA soil conditioner microspheres	572:613	GL/CS/MAP-FA soil conditioner microspheres with core-shell structure	572:639	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	0	35	theme	soil	71:74	arg1	microspheres					88:99	soil conditioner microspheres	71:99	soil conditioner microspheres	71:99	Preparation and application properties of sustainable gelatin/chitosan soil conditioner microspheres.					
32439439	7	36	theme	crop	1020:1023	arg1	cultivation					1025:1035	crop cultivation	1020:1035	crop cultivation	1020:1035	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	5	37	theme	Fourier	718:724	arg1	FT-IR					749:753	FT-IR	749:753	FT-IR	749:753	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	5	37	theme	Fourier	718:724	arg1	spectroscopy					735:746	Fourier infrared spectroscopy	718:746	Fourier infrared spectroscopy (FT-IR)	718:754	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	2	38	theme	negative	226:233	arg1	effects					235:241	some negative effects	221:241	some negative effects	221:241	There are some negative effects such as low efficiency, hard to degrade and harmful to the environment when they are used.					
32439439	2	38	theme	negative	226:233	arg1	efficiency					255:264	low efficiency	251:264	low efficiency	251:264	There are some negative effects such as low efficiency, hard to degrade and harmful to the environment when they are used.					
32439439	5	39	theme	shell	878:882	arg1	materials					884:892	GL/CS shell materials	872:892	GL/CS shell materials	872:892	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	5	40	theme	analysis	756:763	arg1	results					765:771	analysis results	756:771	analysis results	756:771	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	8	41	from	load	1210:1213	arg1	microsphere					1236:1246	the microsphere	1232:1246	the microsphere	1232:1246	The load of fertilizer in the microsphere can effectively prolong the release cycle and improve the utilization rate of fertilizer.					
32439439	5	42	theme	materials	884:892	arg1	performance					857:867	the good coating performance	840:867	the good coating performance of GL/CS shell materials	840:892	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	4	43	theme	ammonium	494:501	arg1	MAP					514:516	MAP	514:516	MAP	514:516	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	4	43	theme	ammonium	494:501	arg1	phosphate					503:511	magnesium ammonium phosphate	484:511	magnesium ammonium phosphate (MAP)	484:517	In this work, gelatin (GL), chitosan (CS), magnesium ammonium phosphate (MAP) and fulvic acid (FA) were combined and prepared into GL/CS/MAP-FA soil conditioner microspheres with core-shell structure through emulsion crosslinking method.					
32439439	5	44	dep	microscopy	697:706	arg1	results					765:771	analysis results	756:771	analysis results	756:771	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	8	45	theme	fertilizer	1218:1227	arg1	load					1210:1213	The load	1206:1213	The load of fertilizer in the microsphere	1206:1246	The load of fertilizer in the microsphere can effectively prolong the release cycle and improve the utilization rate of fertilizer.					
32439439	8	46	theme	release	1276:1282	arg1	cycle					1284:1288	the release cycle	1272:1288	the release cycle	1272:1288	The load of fertilizer in the microsphere can effectively prolong the release cycle and improve the utilization rate of fertilizer.					
32439439	5	47	theme	surface	787:793	arg1	composition					795:805	the surface composition	783:805	the surface composition of the microspheres	783:825	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	7	48	theme	good	1074:1077	arg1	biocompatibility					1079:1094	good biocompatibility	1074:1094	good biocompatibility	1074:1094	Water-retention, degradation and crop cultivation showed that these microspheres owned good biocompatibility, which can extend the water-retention time of the soil, settle free heavy metal ions and promote crop growth.					
32439439	5	49	theme	microspheres	814:825	arg1	composition					795:805	the surface composition	783:805	the surface composition of the microspheres	783:825	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	2	50	theme	low	251:253	arg1	efficiency					255:264	low efficiency	251:264	low efficiency	251:264	There are some negative effects such as low efficiency, hard to degrade and harmful to the environment when they are used.					
32439439	5	51	theme	coating	849:855	arg1	performance					857:867	the good coating performance	840:867	the good coating performance of GL/CS shell materials	840:892	Scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) analysis results clarified the surface composition of the microspheres and revealed the good coating performance of GL/CS shell materials.					
32439439	0	52	theme	conditioner	76:86	arg1	microspheres					88:99	soil conditioner microspheres	71:99	soil conditioner microspheres	71:99	Preparation and application properties of sustainable gelatin/chitosan soil conditioner microspheres.					
32439439	0	53	theme	application	16:26	arg1	properties					28:37	Preparation and application properties	0:37	Preparation and application properties of sustainable	0:52	Preparation and application properties of sustainable gelatin/chitosan soil conditioner microspheres.					
32148852	0	0	theme	factor	108:113	arg1	β-1					115:117	transforming growth factor β-1	88:117	transforming growth factor β-1	88:117	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	5	1	theme	loop-to-helix	1113:1125	arg1	transition					1127:1136	a loop-to-helix transition	1111:1136	a loop-to-helix transition in the core jelly-roll fold	1111:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	8	2	theme	glycan	1569:1574	arg1	modification					1576:1587	glycan modification	1569:1587	glycan modification	1569:1587	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	0	3	theme	growth	101:106	arg1	factor					108:113	transforming growth factor	88:113	transforming growth factor β-1	88:117	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	4	4	theme	extended	835:842	arg1	conformation					844:855	a more open and extended conformation	819:855	a more open and extended conformation	819:855	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	8	5	dep	folding	1508:1514	arg1	the					1496:1498	the	1496:1498	the	1496:1498	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	4	6	theme	structure	708:716	arg1	Comparison					682:691	Comparison	682:691	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex	682:796	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	2	7	theme	endogenous	334:343	arg1	sequestration					389:401	sequestration	389:401	sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1	389:516	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	7	theme	endogenous	334:343	arg1	mechanism					365:373	The primary endogenous activity regulatory mechanism	322:373	The primary endogenous activity regulatory mechanism for TGFβ-1	322:384	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	9	8	theme	experiments	1662:1672	arg1	combination					1594:1604	The combination	1590:1604	The combination of crystallography, solution scattering and biochemical experiments reported here	1590:1686	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	5	9	theme	large-scale	939:949	arg1	change					966:971	a large-scale conformational change	937:971	a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold	937:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	9	10	theme	scattering	1635:1644	arg1	combination					1594:1604	The combination	1590:1604	The combination of crystallography, solution scattering and biochemical experiments reported here	1590:1686	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	1	11	theme	attractive	265:274	arg1	target					276:281	an attractive target	262:281	an attractive target for the treatment of several diseases	262:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	9	12	theme	crystallography	1609:1623	arg1	combination					1594:1604	The combination	1590:1604	The combination of crystallography, solution scattering and biochemical experiments reported here	1590:1686	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	6	13	theme	conformational	1172:1185	arg1	change					1187:1192	This conformational change	1167:1192	This conformational change	1167:1192	This conformational change does not appear to include a repositioning of the integrin-binding motif as previously proposed.					
32148852	5	14	theme	conformational	951:964	arg1	change					966:971	a large-scale conformational change	937:971	a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold	937:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	7	15	from	orientations	1369:1380	arg1	solution					1399:1406	solution	1399:1406	solution	1399:1406	X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution.					
32148852	1	16	theme	growth	158:163	arg1	protein					210:216	a secreted signalling protein	188:216	a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases	188:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	1	16	theme	growth	158:163	arg1	factor					165:170	Transforming growth factor β-1	145:174	Transforming growth factor β-1 (TGFβ-1)	145:183	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	1	16	theme	growth	158:163	arg1	TGFβ-1					177:182	TGFβ-1	177:182	TGFβ-1	177:182	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	0	17	theme	unbound	129:135	arg1	states					137:142	unbound states	129:142	unbound states	129:142	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	4	18	theme	LAP	729:731	arg1	structure					708:716	the crystal structure	696:716	the crystal structure of unbound LAP (solved here to 3.5 Å resolution)	696:765	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	9	19	theme	sequestration	1730:1742	arg1	mechanism					1713:1721	the mechanism	1709:1721	the mechanism of LAP sequestration of TGFβ-1	1709:1752	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	6	20	theme	motif	1261:1265	arg1	repositioning					1223:1235	a repositioning	1221:1235	a repositioning of the integrin-binding motif	1221:1265	This conformational change does not appear to include a repositioning of the integrin-binding motif as previously proposed.					
32148852	7	21	from	ensembles	1386:1394	arg1	solution					1399:1406	solution	1399:1406	solution	1399:1406	X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution.					
32148852	7	22	theme	possible	1360:1367	arg1	orientations					1369:1380	possible orientations	1360:1380	possible orientations	1360:1380	X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution.					
32148852	0	23	from	switching	40:48	arg1	peptide					72:78	latency-associated peptide	53:78	latency-associated peptide	53:78	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	6	24	theme	integrin-binding	1244:1259	arg1	motif					1261:1265	the integrin-binding motif	1240:1265	the integrin-binding motif	1240:1265	This conformational change does not appear to include a repositioning of the integrin-binding motif as previously proposed.					
32148852	5	25	theme	binding	914:920	arg1	TGFβ-1					922:927	binding TGFβ-1	914:927	binding TGFβ-1	914:927	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	9	26	theme	TGFβ-1	1747:1752	arg1	sequestration					1730:1742	LAP sequestration	1726:1742	LAP sequestration of TGFβ-1	1726:1752	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	0	27	theme	Structural	0:9	arg1	insights					11:18	Structural insights	0:18	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1	0:117	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	4	28	theme	open	826:829	arg1	conformation					844:855	a more open and extended conformation	819:855	a more open and extended conformation	819:855	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	5	29	theme	TGFβ-1	922:927	arg1	mechanism					901:909	a mechanism	899:909	a mechanism of binding TGFβ-1	899:927	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	7	30	theme	scattering-based	1297:1312	arg1	modelling					1314:1322	X-ray scattering-based modelling	1291:1322	X-ray scattering-based modelling	1291:1322	X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution.					
32148852	1	31	theme	secreted	190:197	arg1	protein					210:216	a secreted signalling protein	188:216	a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases	188:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	1	31	theme	secreted	190:197	arg1	factor					165:170	Transforming growth factor β-1	145:174	Transforming growth factor β-1 (TGFβ-1)	145:183	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	0	32	theme	conformational	25:38	arg1	switching					40:48	conformational switching	25:48	conformational switching in latency-associated peptide between transforming growth factor β-1	25:117	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	8	33	theme	native	1418:1423	arg1	LAP					1425:1427	native LAP	1418:1427	native LAP	1418:1427	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	1	34	theme	signalling	199:208	arg1	protein					210:216	a secreted signalling protein	188:216	a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases	188:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	1	34	theme	signalling	199:208	arg1	factor					165:170	Transforming growth factor β-1	145:174	Transforming growth factor β-1 (TGFβ-1)	145:183	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	4	35	theme	3.5 Å	749:753	arg1	resolution					755:764	3.5 Å resolution	749:764	3.5 Å resolution	749:764	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	5	36	from	transition	1127:1136	arg1	fold					1161:1164	the core jelly-roll fold	1141:1164	the core jelly-roll fold	1141:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	4	37	theme	unbound	721:727	arg1	LAP					729:731	unbound LAP	721:731	unbound LAP	721:731	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	9	38	theme	fundamental	1765:1775	arg1	importance					1777:1786	fundamental importance	1765:1786	fundamental importance	1765:1786	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	4	39	with	Comparison	682:691	arg1	that					772:775	that	772:775	that	772:775	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	5	40	theme	inter-monomer	1006:1018	arg1	interface					1020:1028	the inter-monomer interface	1002:1028	the inter-monomer interface	1002:1028	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	2	41	theme	latency-associated	423:440	arg1	LAP					451:453	LAP	451:453	LAP	451:453	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	41	theme	latency-associated	423:440	arg1	pro-peptide					410:420	its pro-peptide	406:420	its pro-peptide	406:420	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	41	theme	latency-associated	423:440	arg1	peptide					442:448	latency-associated peptide	423:448	latency-associated peptide (LAP)	423:454	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	42	theme	receptor	484:491	arg1	binding					493:499	receptor binding	484:499	receptor binding	484:499	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	1	43	theme	several	304:310	arg1	diseases					312:319	several diseases	304:319	several diseases	304:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	8	44	theme	overall	1500:1506	arg1	folding					1508:1514	folding	1508:1514	folding	1508:1514	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	5	45	theme	interface	1020:1028	arg1	contraction					987:997	contraction	987:997	contraction of the inter-monomer interface	987:1028	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	5	45	theme	interface	1020:1028	arg1	caging					1034:1039	caging	1034:1039	caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold	1034:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	1	46	theme	diseases	312:319	arg1	treatment					291:299	the treatment	287:299	the treatment of several diseases	287:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	9	47	theme	biochemical	1650:1660	arg1	experiments					1662:1672	biochemical experiments	1650:1672	biochemical experiments reported here	1650:1686	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	3	48	theme	binding	646:652	arg1	mechanism					633:641	the mechanism	629:641	the mechanism of binding	629:652	As such, recombinant LAP is promising as a protein-based therapeutic for modulating TGFβ-1 activity; however, the mechanism of binding is incompletely understood.					
32148852	8	49	theme	unbound	1535:1541	arg1	LAP					1543:1545	unbound LAP	1535:1545	unbound LAP	1535:1545	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	2	50	theme	regulatory	354:363	arg1	sequestration					389:401	sequestration	389:401	sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1	389:516	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	50	theme	regulatory	354:363	arg1	mechanism					365:373	The primary endogenous activity regulatory mechanism	322:373	The primary endogenous activity regulatory mechanism for TGFβ-1	322:384	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	0	51	theme	latency-associated	53:70	arg1	peptide					72:78	latency-associated peptide	53:78	latency-associated peptide	53:78	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	5	52	theme	jelly-roll	1150:1159	arg1	fold					1161:1164	the core jelly-roll fold	1141:1164	the core jelly-roll fold	1141:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	9	53	theme	therapeutic	1792:1802	arg1	development					1804:1814	therapeutic development	1792:1814	therapeutic development	1792:1814	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	3	54	theme	TGFβ-1	603:608	arg1	activity					610:617	TGFβ-1 activity	603:617	TGFβ-1 activity	603:617	As such, recombinant LAP is promising as a protein-based therapeutic for modulating TGFβ-1 activity; however, the mechanism of binding is incompletely understood.					
32148852	9	55	theme	LAP	1726:1728	arg1	sequestration					1730:1742	LAP sequestration	1726:1742	LAP sequestration of TGFβ-1	1726:1752	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	2	56	theme	activity	345:352	arg1	sequestration					389:401	sequestration	389:401	sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1	389:516	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	56	theme	activity	345:352	arg1	mechanism					365:373	The primary endogenous activity regulatory mechanism	322:373	The primary endogenous activity regulatory mechanism for TGFβ-1	322:384	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	8	57	theme	scattering	1463:1472	arg1	experiments					1474:1484	solution scattering experiments	1454:1484	solution scattering experiments	1454:1484	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	8	58	theme	solution	1454:1461	arg1	experiments					1474:1484	solution scattering experiments	1454:1484	solution scattering experiments	1454:1484	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	4	59	theme	bound	784:788	arg1	complex					790:796	the bound complex	780:796	the bound complex	780:796	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	8	60	gly	glycosylated	1440:1451	arg1	LAP					1425:1427	native LAP	1418:1427	native LAP	1418:1427	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	1	61	theme	Transforming	145:156	arg1	protein					210:216	a secreted signalling protein	188:216	a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases	188:319	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	1	61	theme	Transforming	145:156	arg1	factor					165:170	Transforming growth factor β-1	145:174	Transforming growth factor β-1 (TGFβ-1)	145:183	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	1	61	theme	Transforming	145:156	arg1	TGFβ-1					177:182	TGFβ-1	177:182	TGFβ-1	177:182	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	9	62	theme	solution	1626:1633	arg1	scattering					1635:1644	solution scattering	1626:1644	solution scattering	1626:1644	The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.					
32148852	1	63	theme	many	231:234	arg1	processes					245:253	many cellular processes	231:253	many cellular processes	231:253	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	2	64	theme	primary	326:332	arg1	sequestration					389:401	sequestration	389:401	sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1	389:516	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	2	64	theme	primary	326:332	arg1	mechanism					365:373	The primary endogenous activity regulatory mechanism	322:373	The primary endogenous activity regulatory mechanism for TGFβ-1	322:384	The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1.					
32148852	1	65	theme	cellular	236:243	arg1	processes					245:253	many cellular processes	231:253	many cellular processes	231:253	Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases.					
32148852	0	66	theme	transforming	88:99	arg1	factor					108:113	transforming growth factor	88:113	transforming growth factor β-1	88:117	Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states.					
32148852	5	67	theme	straight-jacket	1049:1063	arg1	domain					1066:1071	the 'straight-jacket' domain	1044:1071	the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold	1044:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
32148852	4	68	theme	crystal	700:706	arg1	structure					708:716	the crystal structure	696:716	the crystal structure of unbound LAP (solved here to 3.5 Å resolution)	696:765	Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1.					
32148852	8	69	theme	LAP	1543:1545	arg1	flexibility					1520:1530	flexibility	1520:1530	flexibility	1520:1530	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	8	69	theme	LAP	1543:1545	arg1	folding					1508:1514	folding	1508:1514	folding	1508:1514	Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification.					
32148852	7	70	theme	X-ray	1291:1295	arg1	modelling					1314:1322	X-ray scattering-based modelling	1291:1322	X-ray scattering-based modelling	1291:1322	X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution.					
32148852	3	71	theme	recombinant	528:538	arg1	LAP					540:542	recombinant LAP	528:542	recombinant LAP	528:542	As such, recombinant LAP is promising as a protein-based therapeutic for modulating TGFβ-1 activity; however, the mechanism of binding is incompletely understood.					
32148852	5	72	theme	core	1145:1148	arg1	fold					1161:1164	the core jelly-roll fold	1141:1164	the core jelly-roll fold	1141:1164	Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the 'straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold.					
33244234	2	0	from	resistance	212:221	arg1	cancer					232:237	cancer	232:237	cancer	232:237	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	1	1	with	patients	180:187	arg1	cancer					194:199	cancer	194:199	cancer	194:199	INTRODUCTION Chemoresistance leads to chemotherapy failure in patients with cancer.					
33244234	8	2	theme	reverse	1206:1212	arg1	resistance					1219:1228	reverse drug resistance	1206:1228	reverse drug resistance in cancer	1206:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	6	3	contain	had	911:913	arg2	consumption					930:940	higher glucose consumption	915:940	higher glucose consumption with higher ATPase expression	915:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	6	3	contain	had	911:913	arg2	dependence					993:1002	stronger glucose dependence	976:1002	stronger glucose dependence	976:1002	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	6	3	contain	had	911:913	arg1	cells					905:909	RESULTS Chemoresistant cells	882:909	RESULTS Chemoresistant cells	882:909	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	4	4	from	supply	546:551	arg1	environment					569:579	the cellular environment	556:579	the cellular environment	556:579	Herein, we aimed to reverse resistance by reducing the glucose supply in the cellular environment.					
33244234	6	5	theme	RESULTS	882:888	arg1	cells					905:909	RESULTS Chemoresistant cells	882:909	RESULTS Chemoresistant cells	882:909	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	8	6	from	resistance	1219:1228	arg1	cancer					1233:1238	cancer	1233:1238	cancer	1233:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	5	7	theme	chemoresistant	762:775	arg1	cells					777:781	chemoresistant cells	762:781	chemoresistant cells	762:781	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	3	8	from	cells	431:435	arg1	cancer					440:445	cancer	440:445	cancer	440:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	8	9	theme	CONCLUSION	1145:1154	arg1	therapy					1167:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	8	9	theme	CONCLUSION	1145:1154	arg1	method					1196:1201	a new method	1190:1201	a new method to reverse drug resistance in cancer	1190:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	5	10	dep	METHODS	582:588	arg1	applied					645:651	applied	645:651	applied	645:651	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	0	11	theme	Dextran	95:101	arg1	Nanoparticles					103:115	Dextran Nanoparticles	95:115	Dextran Nanoparticles	95:115	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles.					
33244234	6	12	theme	stronger	976:983	arg1	dependence					993:1002	stronger glucose dependence	976:1002	stronger glucose dependence	976:1002	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	1	13	from	failure	169:175	arg1	patients					180:187	patients	180:187	patients with cancer	180:199	INTRODUCTION Chemoresistance leads to chemotherapy failure in patients with cancer.					
33244234	6	14	with	dependence	993:1002	arg1	expression					961:970	higher ATPase expression	947:970	higher ATPase expression	947:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	7	15	theme	cancer	1048:1053	arg1	cells					1055:1059	cancer cells	1048:1059	cancer cells cultured in a low-glucose condition	1048:1095	Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.					
33244234	2	16	theme	P-glycoprotein	282:295	arg1	expression					268:277	the high expression	259:277	the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease	259:356	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	6	17	with	consumption	930:940	arg1	expression					961:970	higher ATPase expression	947:970	higher ATPase expression	947:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	2	18	gly	P-glycoprotein	282:295	arg1	P-glycoprotein					282:295	P-glycoprotein	282:295	P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease	282:356	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	0	19	theme	Cancer	34:39	arg1	Chemoresistance					41:55	Cancer Chemoresistance	34:55	Cancer Chemoresistance	34:55	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles.					
33244234	5	20	theme	low-glucose	859:869	arg1	condition					871:879	a low-glucose condition	857:879	a low-glucose condition	857:879	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	6	21	theme	ATPase	954:959	arg1	expression					961:970	higher ATPase expression	947:970	higher ATPase expression	947:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	5	22	theme	chemoresistant/chemosensitive	790:818	arg1	mixture					825:831	the chemoresistant/chemosensitive cell mixture	786:831	the chemoresistant/chemosensitive cell mixture	786:831	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	3	23	theme	migration	393:401	arg1	abilities					403:411	the stronger invasion and migration abilities	367:411	the stronger invasion and migration abilities of chemoresistant cells in cancer	367:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	6	24	theme	chemosensitive	1016:1029	arg1	cells					1031:1035	chemosensitive cells	1016:1035	chemosensitive cells	1016:1035	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	3	25	theme	stronger	371:378	arg1	invasion					380:387	stronger invasion	371:387	stronger invasion	371:387	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	5	26	theme	starvation	592:601	arg1	approach					603:610	A starvation approach	590:610	A starvation approach in reversing chemoresistance	590:639	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	2	27	theme	MDR1	312:315	arg1	gene					317:320	the MDR1 gene	308:320	the MDR1 gene	308:320	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	2	27	theme	MDR1	312:315	arg1	protease					349:356	an ATP-dependent protease	332:356	an ATP-dependent protease	332:356	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	3	28	theme	invasion	380:387	arg1	abilities					403:411	the stronger invasion and migration abilities	367:411	the stronger invasion and migration abilities of chemoresistant cells in cancer	367:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	6	29	theme	higher	947:952	arg1	expression					961:970	higher ATPase expression	947:970	higher ATPase expression	947:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	1	30	theme	INTRODUCTION	118:129	arg1	Chemoresistance					131:145	INTRODUCTION Chemoresistance	118:145	INTRODUCTION Chemoresistance	118:145	INTRODUCTION Chemoresistance leads to chemotherapy failure in patients with cancer.					
33244234	3	31	theme	chemoresistant	416:429	arg1	cells					431:435	chemoresistant cells	416:435	chemoresistant cells in cancer	416:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	4	32	theme	cellular	560:567	arg1	environment					569:579	the cellular environment	556:579	the cellular environment	556:579	Herein, we aimed to reverse resistance by reducing the glucose supply in the cellular environment.					
33244234	8	33	theme	new	1192:1194	arg1	therapy					1167:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	8	33	theme	new	1192:1194	arg1	method					1196:1201	a new method	1190:1201	a new method to reverse drug resistance in cancer	1190:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	2	34	theme	Multidrug	202:210	arg1	MDR					224:226	MDR	224:226	MDR	224:226	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	2	34	theme	Multidrug	202:210	arg1	resistance					212:221	Multidrug resistance	202:221	Multidrug resistance (MDR) in cancer	202:237	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	3	35	theme	cells	431:435	arg1	abilities					403:411	the stronger invasion and migration abilities	367:411	the stronger invasion and migration abilities of chemoresistant cells in cancer	367:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	8	36	used	used	1182:1185	arg2	therapy					1167:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	8	36	used	used	1182:1185	arg2	method					1196:1201	a new method	1190:1201	a new method to reverse drug resistance in cancer	1190:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	3	37	theme	ATP	466:468	arg1	consumption					470:480	more ATP consumption	461:480	more ATP consumption	461:480	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	5	38	theme	fluorescent	694:704	arg1	nanoparticles					720:732	fluorescent dextran-based nanoparticles	694:732	fluorescent dextran-based nanoparticles	694:732	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	6	39	theme	glucose	922:928	arg1	consumption					930:940	higher glucose consumption	915:940	higher glucose consumption with higher ATPase expression	915:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	8	40	theme	drug	1214:1217	arg1	resistance					1219:1228	reverse drug resistance	1206:1228	reverse drug resistance in cancer	1206:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	2	41	theme	ATP-dependent	335:347	arg1	gene					317:320	the MDR1 gene	308:320	the MDR1 gene	308:320	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	2	41	theme	ATP-dependent	335:347	arg1	protease					349:356	an ATP-dependent protease	332:356	an ATP-dependent protease	332:356	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	6	42	theme	glucose	985:991	arg1	dependence					993:1002	stronger glucose dependence	976:1002	stronger glucose dependence	976:1002	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	5	43	theme	dextran-based	706:718	arg1	nanoparticles					720:732	fluorescent dextran-based nanoparticles	694:732	fluorescent dextran-based nanoparticles	694:732	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	6	44	theme	higher	915:920	arg1	consumption					930:940	higher glucose consumption	915:940	higher glucose consumption with higher ATPase expression	915:970	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	8	45	theme	Starvation	1156:1165	arg1	therapy					1167:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy	1145:1173	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	8	45	theme	Starvation	1156:1165	arg1	method					1196:1201	a new method	1190:1201	a new method to reverse drug resistance in cancer	1190:1238	CONCLUSION Starvation therapy can be used as a new method to reverse drug resistance in cancer.					
33244234	4	46	theme	glucose	538:544	arg1	supply					546:551	the glucose supply	534:551	the glucose supply in the cellular environment	534:579	Herein, we aimed to reverse resistance by reducing the glucose supply in the cellular environment.					
33244234	7	47	theme	chemoresistant	1123:1136	arg1	cells					1138:1142	chemoresistant cells	1123:1142	chemoresistant cells	1123:1142	Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.					
33244234	7	48	theme	cells	1138:1142	arg1	proportion					1109:1118	the proportion	1105:1118	the proportion of chemoresistant cells	1105:1142	Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.					
33244234	2	49	theme	high	263:266	arg1	expression					268:277	the high expression	259:277	the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease	259:356	Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease.					
33244234	7	50	theme	low-glucose	1075:1085	arg1	condition					1087:1095	a low-glucose condition	1073:1095	a low-glucose condition	1073:1095	Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.					
33244234	1	51	theme	chemotherapy	156:167	arg1	failure					169:175	chemotherapy failure	156:175	chemotherapy failure in patients with cancer	156:199	INTRODUCTION Chemoresistance leads to chemotherapy failure in patients with cancer.					
33244234	0	52	theme	Drug-Resistance	66:80	arg1	Detection					82:90	Drug-Resistance Detection	66:90	Drug-Resistance Detection by Dextran Nanoparticles	66:115	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles.					
33244234	6	53	theme	Chemoresistant	890:903	arg1	cells					905:909	RESULTS Chemoresistant cells	882:909	RESULTS Chemoresistant cells	882:909	RESULTS Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells.					
33244234	3	54	from	cancer	440:445	arg1	abilities					403:411	the stronger invasion and migration abilities	367:411	the stronger invasion and migration abilities of chemoresistant cells in cancer	367:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	5	55	theme	cell	820:823	arg1	mixture					825:831	the chemoresistant/chemosensitive cell mixture	786:831	the chemoresistant/chemosensitive cell mixture	786:831	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
33244234	3	56	from	abilities	403:411	arg1	cancer					440:445	cancer	440:445	cancer	440:445	Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption.					
33244234	0	57	theme	Starvation	10:19	arg1	Effect					0:5	Effect	0:5	Effect of Starvation in Reversing Cancer Chemoresistance	0:55	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles.					
33244234	5	58	theme	cells	777:781	arg1	proportion					748:757	the proportion	744:757	the proportion of chemoresistant cells	744:781	METHODS A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.					
34464639	6	0	theme	oxygen	855:860	arg1	property					870:877	good oxygen barrier property	850:877	good oxygen barrier property	850:877	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	6	1	theme	good	850:853	arg1	property					870:877	good oxygen barrier property	850:877	good oxygen barrier property	850:877	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	1	2	theme	hard	148:151	arg1	material					161:168	hard capsule material	148:168	hard capsule material	148:168	A strategy for preparation of self-reinforced starch films for use as hard capsule material is introduced.					
34464639	5	3	theme	films	699:703	arg1	toughness					665:673	toughness	665:673	toughness	665:673	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	5	3	theme	films	699:703	arg1	strain					654:659	strain	654:659	strain	654:659	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	2	4	used	used	272:275	arg2	HCPS					249:252	HCPS	249:252	HCPS	249:252	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	4	used	used	272:275	arg2	starch					241:246	the hydroxypropylated-crosslinked potato starch	200:246	the hydroxypropylated-crosslinked potato starch (HCPS)	200:253	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	1	5	theme	films	131:135	arg1	preparation					93:103	preparation	93:103	preparation of self-reinforced starch films	93:135	A strategy for preparation of self-reinforced starch films for use as hard capsule material is introduced.					
34464639	6	6	contain	had	846:848	arg1	film					836:839	the self-reinforced starch film	809:839	the self-reinforced starch film	809:839	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	6	6	contain	had	846:848	arg2	property					870:877	good oxygen barrier property	850:877	good oxygen barrier property	850:877	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	1	7	theme	capsule	153:159	arg1	material					161:168	hard capsule material	148:168	hard capsule material	148:168	A strategy for preparation of self-reinforced starch films for use as hard capsule material is introduced.					
34464639	5	8	dep	strain	654:659	arg1	The					650:652	The	650:652	The	650:652	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	6	9	theme	starch	829:834	arg1	film					836:839	the self-reinforced starch film	809:839	the self-reinforced starch film	809:839	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	4	10	theme	matrix/particle	551:565	arg1	interface					567:575	the matrix/particle interface	547:575	the matrix/particle interface	547:575	It was found that the matrix/particle interface was greatly improved after the hydroxypropylation of crosslinked starch.					
34464639	7	11	theme	hard	1095:1098	arg1	capsules					1100:1107	hard capsules	1095:1107	hard capsules	1095:1107	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	6	12	theme	self-reinforced	813:827	arg1	film					836:839	the self-reinforced starch film	809:839	the self-reinforced starch film	809:839	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	6	13	from	5.09 × 10-12 cm3·cm/cm2·s·cmHg	928:957	arg1	OPC					920:922	OPC	920:922	OPC	920:922	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	6	13	from	5.09 × 10-12 cm3·cm/cm2·s·cmHg	928:957	arg1	coefficient					907:917	its oxygen permeating coefficient	885:917	its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH)	885:966	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	2	14	theme	hydroxypropylated-hydrolyzed	294:321	arg1	HHPS					338:341	HHPS	338:341	HHPS	338:341	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	14	theme	hydroxypropylated-hydrolyzed	294:321	arg1	starch					330:335	the hydroxypropylated-hydrolyzed potato starch	290:335	the hydroxypropylated-hydrolyzed potato starch (HHPS) films	290:348	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	7	15	theme	fragmentation	973:985	arg1	rate					987:990	The fragmentation rate	969:990	The fragmentation rate of starch capsules	969:1009	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	3	16	theme	starch	375:380	arg1	samples					382:388	starch samples	375:388	starch samples	375:388	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	0	17	theme	starch	31:36	arg1	films					38:42	self-reinforced starch films	15:42	self-reinforced starch films for use as hard	15:58	Preparation of self-reinforced starch films for use as hard capsule material.					
34464639	4	18	theme	crosslinked	630:640	arg1	starch					642:647	crosslinked starch	630:647	crosslinked starch	630:647	It was found that the matrix/particle interface was greatly improved after the hydroxypropylation of crosslinked starch.					
34464639	3	19	theme	starch	412:417	arg1	films					419:423	starch films	412:423	starch films	412:423	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	20	theme	paste	355:359	arg1	properties					361:370	The paste properties	351:370	The paste properties of starch samples	351:388	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	5	21	theme	10 wt	759:763	arg1	%					764:764	%	764:764	%	764:764	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	7	22	theme	starch	995:1000	arg1	capsules					1002:1009	starch capsules	995:1009	starch capsules	995:1009	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	5	23	theme	particles	771:779	arg1	addition					747:754	addition	747:754	addition of 10 wt% HCPS particles	747:779	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	0	24	theme	self-reinforced	15:29	arg1	films					38:42	self-reinforced starch films	15:42	self-reinforced starch films for use as hard	15:58	Preparation of self-reinforced starch films for use as hard capsule material.					
34464639	6	25	theme	permeating	896:905	arg1	OPC					920:922	OPC	920:922	OPC	920:922	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	6	25	theme	permeating	896:905	arg1	coefficient					907:917	its oxygen permeating coefficient	885:917	its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH)	885:966	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	7	26	theme	capsules	1002:1009	arg1	rate					987:990	The fragmentation rate	969:990	The fragmentation rate of starch capsules	969:1009	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	3	27	theme	rapid	448:452	arg1	RVA					470:472	RVA	470:472	RVA	470:472	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	27	theme	rapid	448:452	arg1	analyzer					460:467	a rapid visco analyzer	446:467	a rapid visco analyzer (RVA)	446:473	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	6	28	theme	oxygen	889:894	arg1	OPC					920:922	OPC	920:922	OPC	920:922	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	6	28	theme	oxygen	889:894	arg1	coefficient					907:917	its oxygen permeating coefficient	885:917	its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH)	885:966	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	5	29	theme	starch	682:687	arg1	films					699:703	the starch composite films	678:703	the starch composite films	678:703	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	7	30	theme	capsules	1100:1107	arg1	preparation					1080:1090	the preparation	1076:1090	the preparation of hard capsules	1076:1107	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	2	31	theme	potato	234:239	arg1	HCPS					249:252	HCPS	249:252	HCPS	249:252	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	31	theme	potato	234:239	arg1	starch					241:246	the hydroxypropylated-crosslinked potato starch	200:246	the hydroxypropylated-crosslinked potato starch (HCPS)	200:253	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	4	32	theme	starch	642:647	arg1	hydroxypropylation					608:625	the hydroxypropylation	604:625	the hydroxypropylation of crosslinked starch	604:647	It was found that the matrix/particle interface was greatly improved after the hydroxypropylation of crosslinked starch.					
34464639	3	33	theme	films	419:423	arg1	properties					361:370	The paste properties	351:370	The paste properties of starch samples	351:388	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	33	theme	films	419:423	arg1	morphology					398:407	the morphology	394:407	the morphology of starch films	394:423	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	0	34	theme	films	38:42	arg1	Preparation					0:10	Preparation	0:10	Preparation of self-reinforced starch films for use as hard	0:58	Preparation of self-reinforced starch films for use as hard capsule material.					
34464639	2	35	theme	hydroxypropylated-crosslinked	204:232	arg1	HCPS					249:252	HCPS	249:252	HCPS	249:252	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	35	theme	hydroxypropylated-crosslinked	204:232	arg1	starch					241:246	the hydroxypropylated-crosslinked potato starch	200:246	the hydroxypropylated-crosslinked potato starch (HCPS)	200:253	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	5	36	theme	%	764:764	arg1	particles					771:779	10 wt% HCPS particles	759:779	10 wt% HCPS particles	759:779	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	3	37	theme	samples	382:388	arg1	properties					361:370	The paste properties	351:370	The paste properties of starch samples	351:388	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	37	theme	samples	382:388	arg1	morphology					398:407	the morphology	394:407	the morphology of starch films	394:423	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	4	38	link	crosslinked	630:640	arg1	starch					642:647	crosslinked starch	630:647	crosslinked starch	630:647	It was found that the matrix/particle interface was greatly improved after the hydroxypropylation of crosslinked starch.					
34464639	5	39	theme	composite	689:697	arg1	films					699:703	the starch composite films	678:703	the starch composite films	678:703	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	3	40	theme	visco	454:458	arg1	RVA					470:472	RVA	470:472	RVA	470:472	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	40	theme	visco	454:458	arg1	analyzer					460:467	a rapid visco analyzer	446:467	a rapid visco analyzer (RVA)	446:473	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	1	41	theme	self-reinforced	108:122	arg1	films					131:135	self-reinforced starch films	108:135	self-reinforced starch films	108:135	A strategy for preparation of self-reinforced starch films for use as hard capsule material is introduced.					
34464639	1	42	theme	starch	124:129	arg1	films					131:135	self-reinforced starch films	108:135	self-reinforced starch films	108:135	A strategy for preparation of self-reinforced starch films for use as hard capsule material is introduced.					
34464639	2	43	theme	potato	323:328	arg1	HHPS					338:341	HHPS	338:341	HHPS	338:341	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	43	theme	potato	323:328	arg1	starch					330:335	the hydroxypropylated-hydrolyzed potato starch	290:335	the hydroxypropylated-hydrolyzed potato starch (HHPS) films	290:348	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	44	theme	starch	330:335	arg1	films					344:348	the hydroxypropylated-hydrolyzed potato starch (HHPS) films	290:348	the hydroxypropylated-hydrolyzed potato starch (HHPS) films	290:348	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	3	45	theme	scanning	479:486	arg1	SEM					509:511	SEM	509:511	SEM	509:511	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	45	theme	scanning	479:486	arg1	microscope					497:506	scanning electron microscope	479:506	scanning electron microscope (SEM)	479:512	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	7	46	theme	alternative	1051:1061	arg1	it					1042:1043	it	1042:1043	it	1042:1043	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	7	46	theme	alternative	1051:1061	arg1	material					1063:1070	an alternative material	1048:1070	an alternative material for the preparation of hard capsules	1048:1107	The fragmentation rate of starch capsules has also decreased, indicating it is an alternative material for the preparation of hard capsules.					
34464639	6	47	theme	barrier	862:868	arg1	property					870:877	good oxygen barrier property	850:877	good oxygen barrier property	850:877	In addition, the self-reinforced starch film also had good oxygen barrier property, with its oxygen permeating coefficient (OPC) at 5.09 × 10-12 cm3·cm/cm2·s·cmHg (50% RH).					
34464639	5	48	theme	HCPS	766:769	arg1	particles					771:779	10 wt% HCPS particles	759:779	10 wt% HCPS particles	759:779	The strain and toughness of the starch composite films were increased by about 30% and 50% after addition of 10 wt% HCPS particles, respectively.					
34464639	3	49	theme	electron	488:495	arg1	SEM					509:511	SEM	509:511	SEM	509:511	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	3	49	theme	electron	488:495	arg1	microscope					497:506	scanning electron microscope	479:506	scanning electron microscope (SEM)	479:512	The paste properties of starch samples and the morphology of starch films were investigated by a rapid visco analyzer (RVA) and scanning electron microscope (SEM), respectively.					
34464639	2	50	link	hydroxypropylated-crosslinked	204:232	arg1	HCPS					249:252	HCPS	249:252	HCPS	249:252	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34464639	2	50	link	hydroxypropylated-crosslinked	204:232	arg1	starch					241:246	the hydroxypropylated-crosslinked potato starch	200:246	the hydroxypropylated-crosslinked potato starch (HCPS)	200:253	In this study, the hydroxypropylated-crosslinked potato starch (HCPS) was prepared and used to reinforce the hydroxypropylated-hydrolyzed potato starch (HHPS) films.					
34360944	10	0	from	endothelia	1585:1594	arg1	setting					1550:1556	the setting	1546:1556	the setting of paracellular studies in endothelia and epithelia	1546:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	7	1	from	nanoscale	1043:1051	arg1	microscopy					1000:1009	single molecule localization microscopy	971:1009	single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale	971:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	1	from	nanoscale	1043:1051	arg1	CLDN5					1030:1034	CLDN5	1030:1034	CLDN5	1030:1034	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	1	from	nanoscale	1043:1051	arg1	SMLM					1012:1015	SMLM	1012:1015	SMLM	1012:1015	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	1	from	nanoscale	1043:1051	arg1	ZO-1					1021:1024	ZO-1	1021:1024	ZO-1	1021:1024	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	9	2	theme	non-random	1401:1410	arg1	clustering					1422:1431	non-random increased clustering	1401:1431	non-random increased clustering of CLDN5	1401:1440	At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5.					
34360944	6	3	theme	immunofluorescence	900:917	arg1	imaging					919:925	automated immunofluorescence imaging	890:925	automated immunofluorescence imaging	890:925	Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging.					
34360944	10	4	theme	studies	1574:1580	arg1	setting					1550:1556	the setting	1546:1556	the setting of paracellular studies in endothelia and epithelia	1546:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	5	5	theme	10	679:680	arg1	kDa					682:684	kDa	682:684	kDa	682:684	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	3	6	theme	monolayer	547:555	arg1	setting					510:516	the setting	506:516	the setting of a single endothelial cell monolayer	506:555	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	1	7	theme	processes	269:277	arg1	maintenance					240:250	the maintenance	236:250	the maintenance of physiological processes	236:277	Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes.					
34360944	8	8	theme	AlaGln	1173:1178	arg1	application					1158:1168	the application	1154:1168	the application of AlaGln	1154:1178	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	5	9	theme	electrical	647:656	arg1	resistance					658:667	Transendothelial electrical resistance	630:667	Transendothelial electrical resistance (TER)	630:673	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	5	9	theme	electrical	647:656	arg1	TER					670:672	TER	670:672	TER	670:672	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	3	10	theme	endothelial	530:540	arg1	monolayer					547:555	a single endothelial cell monolayer	521:555	a single endothelial cell monolayer	521:555	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	9	11	theme	CLDN5	1436:1440	arg1	clustering					1422:1431	non-random increased clustering	1401:1431	non-random increased clustering of CLDN5	1401:1440	At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5.					
34360944	0	12	theme	Cell	143:146	arg1	Monolayer					148:156	a Single Endothelial Cell Monolayer	122:156	a Single Endothelial Cell Monolayer	122:156	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	7	13	from	microscopy	1000:1009	arg1	nanoscale					1043:1051	nanoscale	1043:1051	nanoscale	1043:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	6	14	theme	Single	787:792	arg1	monolayers					794:803	Single monolayers	787:803	Single monolayers	787:803	Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging.					
34360944	7	15	theme	clustering	1065:1074	arg1	analysis					1076:1083	spatial clustering analysis	1057:1083	spatial clustering analysis	1057:1083	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	1	16	theme	epithelial	193:202	arg1	function					212:219	epithelial barrier function	193:219	epithelial barrier function	193:219	Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes.					
34360944	0	17	theme	Single	124:129	arg1	Monolayer					148:156	a Single Endothelial Cell Monolayer	122:156	a Single Endothelial Cell Monolayer	122:156	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	8	18	from	increase	1246:1253	arg1	ZO-1					1262:1265	ZO-1	1262:1265	ZO-1	1262:1265	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	8	18	from	increase	1246:1253	arg1	abundance					1277:1285	CLDN5 abundance	1271:1285	CLDN5 abundance	1271:1285	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	10	19	from	epithelia	1600:1608	arg1	setting					1550:1556	the setting	1546:1556	the setting of paracellular studies in endothelia and epithelia	1546:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	5	20	theme	FITC	686:689	arg1	dextran					691:697	10 kDa FITC dextran	679:697	10 kDa FITC dextran flux	679:702	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	7	21	theme	CLDN5	1030:1034	arg1	microscopy					1000:1009	single molecule localization microscopy	971:1009	single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale	971:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	21	theme	CLDN5	1030:1034	arg1	SMLM					1012:1015	SMLM	1012:1015	SMLM	1012:1015	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	3	22	theme	cell	542:545	arg1	monolayer					547:555	a single endothelial cell monolayer	521:555	a single endothelial cell monolayer	521:555	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	1	23	theme	physiological	255:267	arg1	processes					269:277	physiological processes	255:277	physiological processes	255:277	Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes.					
34360944	7	24	theme	molecule	978:985	arg1	microscopy					1000:1009	single molecule localization microscopy	971:1009	single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale	971:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	24	theme	molecule	978:985	arg1	SMLM					1012:1015	SMLM	1012:1015	SMLM	1012:1015	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	10	25	from	monolayer	1506:1514	arg1	data					1487:1490	multiple data	1478:1490	multiple data from a single monolayer	1478:1514	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	0	26	theme	Junction	81:88	arg1	Composition					90:100	Tight Junction Composition	75:100	Tight Junction Composition	75:100	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	2	27	theme	barrier	284:290	arg1	permeability					305:316	The barrier paracellular permeability	280:316	The barrier paracellular permeability	280:316	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	8	28	from	abundance	1277:1285	arg1	interface					1304:1312	the cell-cell interface	1290:1312	the cell-cell interface	1290:1312	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	8	29	dep	ZO-1	1262:1265	arg1	the					1258:1260	the	1258:1260	the	1258:1260	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	7	30	used	used	962:965	arg2	monolayers					946:955	the same monolayers	937:955	the same monolayers	937:955	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	10	31	theme	multiple	1478:1485	arg1	data					1487:1490	multiple data	1478:1490	multiple data from a single monolayer	1478:1514	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	3	32	theme	experimental	432:443	arg1	workflow					445:452	an experimental workflow	429:452	an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer	429:555	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	7	33	theme	same	941:944	arg1	monolayers					946:955	the same monolayers	937:955	the same monolayers	937:955	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	10	34	theme	single	1499:1504	arg1	monolayer					1506:1514	a single monolayer	1497:1514	a single monolayer	1497:1514	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	3	35	theme	several	466:472	arg1	layers					474:479	several layers	466:479	several layers of physiological data	466:501	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	4	36	theme	umbilical	564:572	arg1	cells					591:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells were grown on Transwell filters.					
34360944	10	37	from	setting	1550:1556	arg1	epithelia					1600:1608	epithelia	1600:1608	epithelia	1600:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	10	37	from	setting	1550:1556	arg1	endothelia					1585:1594	endothelia	1585:1594	endothelia	1585:1594	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	10	38	theme	wide	1524:1527	arg1	applicability					1529:1541	wide applicability	1524:1541	wide applicability in the setting of paracellular studies in endothelia and epithelia	1524:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	6	39	used	used	881:884	arg2	monolayers					794:803	Single monolayers	787:803	Single monolayers	787:803	Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging.					
34360944	3	40	theme	data	498:501	arg1	layers					474:479	several layers	466:479	several layers of physiological data	466:501	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	5	41	theme	paracellular	755:766	arg1	Alanyl-Glutamine					724:739	Alanyl-Glutamine	724:739	Alanyl-Glutamine (AlaGln)	724:748	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	5	41	theme	paracellular	755:766	arg1	modulator					776:784	a paracellular barrier modulator	753:784	a paracellular barrier modulator	753:784	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	9	42	theme	abundance	1366:1374	arg1	data					1376:1379	the functional and protein abundance data	1339:1379	data	1376:1379	At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5.					
34360944	0	43	dep	Workflow	16:23	arg1	Proof					159:163	Proof	159:163	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.	0:175	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	2	44	theme	tight	390:394	arg1	TJ					407:408	TJ	407:408	TJ	407:408	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	2	44	theme	tight	390:394	arg1	junctions					396:404	the cell-to-cell tight junctions	373:404	the cell-to-cell tight junctions (TJ)	373:409	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	8	45	theme	dextran	1125:1131	arg1	flux					1133:1136	the paracellular dextran flux	1108:1136	the paracellular dextran flux	1108:1136	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	5	46	theme	barrier	768:774	arg1	Alanyl-Glutamine					724:739	Alanyl-Glutamine	724:739	Alanyl-Glutamine (AlaGln)	724:748	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	5	46	theme	barrier	768:774	arg1	modulator					776:784	a paracellular barrier modulator	753:784	a paracellular barrier modulator	753:784	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	0	47	theme	Concept	168:174	arg1	Proof					159:163	Proof	159:163	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.	0:175	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	4	48	theme	endothelial	579:589	arg1	cells					591:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells were grown on Transwell filters.					
34360944	6	49	theme	Zonula	829:834	arg1	ZO-1					849:852	ZO-1	849:852	ZO-1	849:852	Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging.					
34360944	6	49	theme	Zonula	829:834	arg1	Occludens-1					836:846	Zonula Occludens-1	829:846	Zonula Occludens-1 (ZO-1)	829:853	Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging.					
34360944	2	50	theme	paracellular	292:303	arg1	permeability					305:316	The barrier paracellular permeability	280:316	The barrier paracellular permeability	280:316	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	9	51	theme	increased	1412:1420	arg1	clustering					1422:1431	non-random increased clustering	1401:1431	non-random increased clustering of CLDN5	1401:1440	At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5.					
34360944	6	52	theme	automated	890:898	arg1	imaging					919:925	automated immunofluorescence imaging	890:925	automated immunofluorescence imaging	890:925	Single monolayers were immunolabelled for Zonula Occludens-1 (ZO-1) and Claudin-5 (CLDN5) and used for automated immunofluorescence imaging.					
34360944	10	53	theme	paracellular	1561:1572	arg1	studies					1574:1580	paracellular studies	1561:1580	paracellular studies in endothelia and epithelia	1561:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	5	54	theme	Transendothelial	630:645	arg1	resistance					658:667	Transendothelial electrical resistance	630:667	Transendothelial electrical resistance (TER)	630:673	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	5	54	theme	Transendothelial	630:645	arg1	TER					670:672	TER	670:672	TER	670:672	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	3	55	theme	single	523:528	arg1	monolayer					547:555	a single endothelial cell monolayer	521:555	a single endothelial cell monolayer	521:555	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	7	56	theme	spatial	1057:1063	arg1	analysis					1076:1083	spatial clustering analysis	1057:1083	spatial clustering analysis	1057:1083	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	8	57	theme	functional	1190:1199	arg1	changes					1201:1207	these functional changes	1184:1207	these functional changes of the monolayer	1184:1224	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	0	58	theme	Endothelial	131:141	arg1	Monolayer					148:156	a Single Endothelial Cell Monolayer	122:156	a Single Endothelial Cell Monolayer	122:156	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	1	59	theme	barrier	204:210	arg1	function					212:219	epithelial barrier function	193:219	epithelial barrier function	193:219	Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes.					
34360944	5	60	theme	kDa	682:684	arg1	dextran					691:697	10 kDa FITC dextran	679:697	10 kDa FITC dextran flux	679:702	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	7	61	theme	ZO-1	1021:1024	arg1	microscopy					1000:1009	single molecule localization microscopy	971:1009	single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale	971:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	61	theme	ZO-1	1021:1024	arg1	SMLM					1012:1015	SMLM	1012:1015	SMLM	1012:1015	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	8	62	theme	monolayer	1216:1224	arg1	changes					1201:1207	these functional changes	1184:1207	these functional changes of the monolayer	1184:1224	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	8	63	theme	CLDN5	1271:1275	arg1	abundance					1277:1285	CLDN5 abundance	1271:1285	CLDN5 abundance	1271:1285	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	5	64	theme	dextran	691:697	arg1	flux					699:702	10 kDa FITC dextran flux	679:702	10 kDa FITC dextran flux	679:702	Transendothelial electrical resistance (TER) and 10 kDa FITC dextran flux were measured using Alanyl-Glutamine (AlaGln) as a paracellular barrier modulator.					
34360944	8	65	from	ZO-1	1262:1265	arg1	interface					1304:1312	the cell-cell interface	1290:1312	the cell-cell interface	1290:1312	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	8	66	theme	cell-cell	1294:1302	arg1	interface					1304:1312	the cell-cell interface	1290:1312	the cell-cell interface	1290:1312	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	0	67	theme	Tight	75:79	arg1	Composition					90:100	Tight Junction Composition	75:100	Tight Junction Composition	75:100	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	7	68	theme	localization	987:998	arg1	microscopy					1000:1009	single molecule localization microscopy	971:1009	single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale	971:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	68	theme	localization	987:998	arg1	SMLM					1012:1015	SMLM	1012:1015	SMLM	1012:1015	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	10	69	contain	has	1520:1522	arg1	workflow					1460:1467	Our experimental workflow	1443:1467	Our experimental workflow	1443:1467	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	10	69	contain	has	1520:1522	arg2	applicability					1529:1541	wide applicability	1524:1541	wide applicability in the setting of paracellular studies in endothelia and epithelia	1524:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	7	70	theme	single	971:976	arg1	microscopy					1000:1009	single molecule localization microscopy	971:1009	single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale	971:1051	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	7	70	theme	single	971:976	arg1	SMLM					1012:1015	SMLM	1012:1015	SMLM	1012:1015	Finally, the same monolayers were used for single molecule localization microscopy (SMLM) of ZO-1 and CLDN5 at the nanoscale for spatial clustering analysis.					
34360944	0	71	theme	Experimental	3:14	arg1	Workflow					16:23	An Experimental Workflow	0:23	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.	0:175	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	4	72	theme	Human	558:562	arg1	cells					591:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells were grown on Transwell filters.					
34360944	10	73	theme	experimental	1447:1458	arg1	workflow					1460:1467	Our experimental workflow	1443:1467	Our experimental workflow	1443:1467	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	2	74	dep	composition	333:343	arg1	the					329:331	the	329:331	the	329:331	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	2	75	theme	junctions	396:404	arg1	composition					333:343	composition	333:343	composition	333:343	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	2	75	theme	junctions	396:404	arg1	distribution					357:368	spatial distribution	349:368	spatial distribution	349:368	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	9	76	theme	nanoscale	1322:1330	arg1	level					1332:1336	the nanoscale level	1318:1336	the nanoscale level	1318:1336	At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5.					
34360944	4	77	theme	vein	574:577	arg1	cells					591:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells	558:595	Human umbilical vein endothelial cells were grown on Transwell filters.					
34360944	0	78	theme	Barrier	38:44	arg1	Integrity					46:54	Barrier Integrity	38:54	Barrier Integrity	38:54	An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept.					
34360944	10	79	from	applicability	1529:1541	arg1	setting					1550:1556	the setting	1546:1556	the setting of paracellular studies in endothelia and epithelia	1546:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	2	80	theme	spatial	349:355	arg1	distribution					357:368	spatial distribution	349:368	spatial distribution	349:368	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	9	81	theme	protein	1358:1364	arg1	data					1376:1379	the functional and protein abundance data	1339:1379	data	1376:1379	At the nanoscale level, the functional and protein abundance data were accompanied by non-random increased clustering of CLDN5.					
34360944	8	82	theme	paracellular	1112:1123	arg1	flux					1133:1136	the paracellular dextran flux	1108:1136	the paracellular dextran flux	1108:1136	The TER increased and the paracellular dextran flux decreased after the application of AlaGln and these functional changes of the monolayer were mediated by an increase in the ZO-1 and CLDN5 abundance in the cell-cell interface.					
34360944	4	83	theme	Transwell	611:619	arg1	filters					621:627	Transwell filters	611:627	Transwell filters	611:627	Human umbilical vein endothelial cells were grown on Transwell filters.					
34360944	3	84	theme	physiological	484:496	arg1	data					498:501	physiological data	484:501	physiological data	484:501	Here, we provide an experimental workflow that yields several layers of physiological data in the setting of a single endothelial cell monolayer.					
34360944	10	85	from	studies	1574:1580	arg1	epithelia					1600:1608	epithelia	1600:1608	epithelia	1600:1608	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	10	85	from	studies	1574:1580	arg1	endothelia					1585:1594	endothelia	1585:1594	endothelia	1585:1594	Our experimental workflow provides multiple data from a single monolayer and has wide applicability in the setting of paracellular studies in endothelia and epithelia.					
34360944	2	86	theme	cell-to-cell	377:388	arg1	TJ					407:408	TJ	407:408	TJ	407:408	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34360944	2	86	theme	cell-to-cell	377:388	arg1	junctions					396:404	the cell-to-cell tight junctions	373:404	the cell-to-cell tight junctions (TJ)	373:409	The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ).					
34980349	0	0	theme	cellulose	91:99	arg1	nanocrystals					101:112	cellulose nanocrystals	91:112	cellulose nanocrystals	91:112	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	3	1	theme	s	717:717	arg1	composition					720:730	the monomer(s) composition	705:730	the monomer(s) composition within the aqueous phase	705:755	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	1	2	theme	cellulose	197:205	arg1	CNCs					221:224	CNCs	221:224	CNCs	221:224	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	1	2	theme	cellulose	197:205	arg1	nanocrystals					207:218	cellulose nanocrystals	197:218	cellulose nanocrystals (CNCs)	197:225	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	0	3	from	Elaboration	0:10	arg1	polymerization					55:68	Pickering double emulsion polymerization	29:68	Pickering double emulsion polymerization	29:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	2	4	theme	oil	394:396	arg1	phase					398:402	oil phase	394:402	oil phase	394:402	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	1	5	theme	double	125:130	arg1	O/W/O					162:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	0	6	from	polymerization	55:68	arg1	capsules					15:22	capsules	15:22	capsules from Pickering double emulsion polymerization	15:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	0	6	from	polymerization	55:68	arg1	Elaboration					0:10	Elaboration	0:10	Elaboration of capsules from Pickering double emulsion polymerization	0:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	6	7	theme	%	1051:1051	arg1	efficiency					1029:1038	an encapsulation efficiency	1012:1038	an encapsulation efficiency of about 90%	1012:1051	Both hydrophobic and hydrophilic model dyes were encapsulated, with an encapsulation efficiency of about 90%.					
34980349	2	8	theme	stabilizing	542:552	arg1	particles					554:562	stabilizing particles	542:562	stabilizing particles	542:562	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	3	9	theme	Formulation	565:575	arg1	parameters					577:586	Formulation parameters	565:586	Formulation parameters	565:586	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	5	10	theme	obtained	828:835	arg1	vessels					935:941	promising encapsulation vessels	911:941	promising encapsulation vessels	911:941	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	5	10	theme	obtained	828:835	arg1	objects					837:843	The obtained objects	824:843	The obtained objects	824:843	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	3	11	theme	aqueous	743:749	arg1	phase					751:755	the aqueous phase	739:755	the aqueous phase	739:755	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	6	12	theme	hydrophobic	949:959	arg1	dyes					983:986	Both hydrophobic and hydrophilic model dyes	944:986	Both hydrophobic and hydrophilic model dyes	944:986	Both hydrophobic and hydrophilic model dyes were encapsulated, with an encapsulation efficiency of about 90%.					
34980349	2	13	theme	glycol	428:433	arg1	methacrylate					435:446	hydroxyl oligoethylene glycol methacrylate	405:446	hydroxyl oligoethylene glycol methacrylate (OEGMA)	405:454	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	13	theme	glycol	428:433	arg1	components					347:356	only bio-friendly components	329:356	only bio-friendly components	329:356	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	13	theme	glycol	428:433	arg1	OEGMA					449:453	OEGMA	449:453	OEGMA	449:453	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	14	theme	glycol	487:492	arg1	TEGDA					506:510	TEGDA	506:510	TEGDA	506:510	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	14	theme	glycol	487:492	arg1	diacrylate					494:503	tetraethylene glycol diacrylate	473:503	tetraethylene glycol diacrylate (TEGDA) as cross-linker	473:527	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	6	15	theme	encapsulation	1015:1027	arg1	efficiency					1029:1038	an encapsulation efficiency	1012:1038	an encapsulation efficiency of about 90%	1012:1051	Both hydrophobic and hydrophilic model dyes were encapsulated, with an encapsulation efficiency of about 90%.					
34980349	3	16	theme	monomer	709:715	arg1	composition					720:730	the monomer(s) composition	705:730	the monomer(s) composition within the aqueous phase	705:755	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	2	17	theme	oligoethylene	414:426	arg1	methacrylate					435:446	hydroxyl oligoethylene glycol methacrylate	405:446	hydroxyl oligoethylene glycol methacrylate (OEGMA)	405:454	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	17	theme	oligoethylene	414:426	arg1	components					347:356	only bio-friendly components	329:356	only bio-friendly components	329:356	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	17	theme	oligoethylene	414:426	arg1	OEGMA					449:453	OEGMA	449:453	OEGMA	449:453	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	18	theme	tetraethylene	473:485	arg1	TEGDA					506:510	TEGDA	506:510	TEGDA	506:510	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	18	theme	tetraethylene	473:485	arg1	diacrylate					494:503	tetraethylene glycol diacrylate	473:503	tetraethylene glycol diacrylate (TEGDA) as cross-linker	473:527	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	1	19	theme	Pickering	115:123	arg1	O/W/O					162:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	0	20	theme	capsules	15:22	arg1	Elaboration					0:10	Elaboration	0:10	Elaboration of capsules from Pickering double emulsion polymerization	0:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	4	21	theme	matrix	807:812	arg1	capsules					814:821	matrix capsules	807:821	matrix capsules	807:821	The latter was further polymerized to synthesize matrix capsules.					
34980349	2	22	theme	bio-friendly	334:345	arg1	methacrylate					435:446	hydroxyl oligoethylene glycol methacrylate	405:446	hydroxyl oligoethylene glycol methacrylate (OEGMA)	405:454	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	22	theme	bio-friendly	334:345	arg1	components					347:356	only bio-friendly components	329:356	only bio-friendly components	329:356	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	22	theme	bio-friendly	334:345	arg1	CNCs					534:537	CNCs	534:537	CNCs as stabilizing particles	534:562	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	5	23	theme	promising	911:919	arg1	vessels					935:941	promising encapsulation vessels	911:941	promising encapsulation vessels	911:941	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	5	23	theme	promising	911:919	arg1	objects					837:843	The obtained objects	824:843	The obtained objects	824:843	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	0	24	theme	double	39:44	arg1	polymerization					55:68	Pickering double emulsion polymerization	29:68	Pickering double emulsion polymerization	29:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	0	25	theme	Pickering	29:37	arg1	polymerization					55:68	Pickering double emulsion polymerization	29:68	Pickering double emulsion polymerization	29:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	3	26	theme	double	673:678	arg1	drops					689:693	the double emulsion drops	669:693	the double emulsion drops	669:693	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	3	27	theme	inner	638:642	arg1	droplets					653:660	inner emulsion droplets	638:660	inner emulsion droplets within the double emulsion drops	638:693	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	5	28	theme	good	852:855	arg1	resistance					857:866	good resistance	852:866	good resistance to the vacuum	852:880	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	3	29	theme	emulsion	680:687	arg1	drops					689:693	the double emulsion drops	669:693	the double emulsion drops	669:693	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	1	30	theme	oil-in-water-in-oil	132:150	arg1	O/W/O					162:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	0	31	theme	emulsion	46:53	arg1	polymerization					55:68	Pickering double emulsion polymerization	29:68	Pickering double emulsion polymerization	29:68	Elaboration of capsules from Pickering double emulsion polymerization stabilized solely by cellulose nanocrystals.					
34980349	1	32	dep	using	184:188	arg1	modified					239:246	modified	239:246	were modified by introducing surface brominated functions	234:290	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	1	33	theme	emulsions	152:160	arg1	O/W/O					162:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O	115:166	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	6	34	theme	model	977:981	arg1	dyes					983:986	Both hydrophobic and hydrophilic model dyes	944:986	Both hydrophobic and hydrophilic model dyes	944:986	Both hydrophobic and hydrophilic model dyes were encapsulated, with an encapsulation efficiency of about 90%.					
34980349	5	35	used	used	903:906	arg2	objects					837:843	The obtained objects	824:843	The obtained objects	824:843	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	5	35	used	used	903:906	arg2	vessels					935:941	promising encapsulation vessels	911:941	promising encapsulation vessels	911:941	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	3	36	theme	emulsion	644:651	arg1	droplets					653:660	inner emulsion droplets	638:660	inner emulsion droplets within the double emulsion drops	638:693	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	2	37	theme	hydroxyl	405:412	arg1	methacrylate					435:446	hydroxyl oligoethylene glycol methacrylate	405:446	hydroxyl oligoethylene glycol methacrylate (OEGMA)	405:454	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	37	theme	hydroxyl	405:412	arg1	components					347:356	only bio-friendly components	329:356	only bio-friendly components	329:356	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	2	37	theme	hydroxyl	405:412	arg1	OEGMA					449:453	OEGMA	449:453	OEGMA	449:453	The emulsions were formulated using only bio-friendly components, among which isopropyl myristate as oil phase, hydroxyl oligoethylene glycol methacrylate (OEGMA) as macromonomer, tetraethylene glycol diacrylate (TEGDA) as cross-linker, and CNCs as stabilizing particles.					
34980349	1	38	theme	surface	263:269	arg1	functions					282:290	surface brominated functions	263:290	surface brominated functions	263:290	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34980349	6	39	theme	hydrophilic	965:975	arg1	dyes					983:986	Both hydrophobic and hydrophilic model dyes	944:986	Both hydrophobic and hydrophilic model dyes	944:986	Both hydrophobic and hydrophilic model dyes were encapsulated, with an encapsulation efficiency of about 90%.					
34980349	3	40	theme	droplets	653:660	arg1	fraction					626:633	the fraction	622:633	the fraction of inner emulsion droplets within the double emulsion drops	622:693	Formulation parameters could be tuned easily to modulate the fraction of inner emulsion droplets within the double emulsion drops or change the monomer(s) composition within the aqueous phase.					
34980349	5	41	theme	encapsulation	921:933	arg1	vessels					935:941	promising encapsulation vessels	911:941	promising encapsulation vessels	911:941	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	5	41	theme	encapsulation	921:933	arg1	objects					837:843	The obtained objects	824:843	The obtained objects	824:843	The obtained objects showed good resistance to the vacuum and were efficiently used as promising encapsulation vessels.					
34980349	1	42	theme	brominated	271:280	arg1	functions					282:290	surface brominated functions	263:290	surface brominated functions	263:290	Pickering double oil-in-water-in-oil emulsions O/W/O were stabilized using solely cellulose nanocrystals (CNCs), which were modified by introducing surface brominated functions.					
34743940	3	0	theme	bone	539:542	arg1	regeneration					544:555	bone regeneration	539:555	bone regeneration	539:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	6	1	theme	fibroin	668:674	arg1	hydrogel					689:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel were characterized.					
34743940	12	2	theme	scaffolding	1440:1450	arg1	material					1452:1459	dental scaffolding material	1433:1459	dental scaffolding material	1433:1459	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	12	3	theme	hydrogel	1421:1428	arg1	use					1383:1385	the potential use	1369:1385	the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material	1369:1459	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	13	4	dep	in	1488:1489	arg1	vivo					1491:1494	vivo	1491:1494	vivo	1491:1494	Further studies including in vivo examinations are needed prior to its clinical application.					
34743940	11	5	theme	Increased	1248:1256	arg1	activity					1262:1269	Increased ALP activity	1248:1269	Increased ALP activity	1248:1269	Increased ALP activity was observed in MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel.					
34743940	2	6	theme	important	319:327	arg1	role					329:332	an important role	316:332	an important role	316:332	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	6	7	theme	HA	664:665	arg1	hydrogel					689:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel were characterized.					
34743940	8	8	theme	degradation	879:889	arg1	peak					891:894	a degradation peak	877:894	a degradation peak of approximately 250 °C. Hydrogel	877:928	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	12	9	theme	potential	1373:1381	arg1	use					1383:1385	the potential use	1369:1385	the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material	1369:1459	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	0	10	theme	Injectable	0:9	arg1	eggshell-derived					11:26	Injectable eggshell-derived	0:26	Injectable eggshell-derived	0:26	Injectable eggshell-derived hydroxyapatite-incorporated fibroin-alginate composite hydrogel for bone tissue engineering.					
34743940	13	11	theme	Further	1462:1468	arg1	examinations					1496:1507	in vivo examinations	1488:1507	in vivo examinations	1488:1507	Further studies including in vivo examinations are needed prior to its clinical application.					
34743940	13	11	theme	Further	1462:1468	arg1	studies					1470:1476	Further studies	1462:1476	Further studies including in vivo examinations	1462:1507	Further studies including in vivo examinations are needed prior to its clinical application.					
34743940	12	12	theme	fibroin-alginate	1404:1419	arg1	hydrogel					1421:1428	injectable HA fibroin-alginate hydrogel	1390:1428	injectable HA fibroin-alginate hydrogel	1390:1428	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	8	13	theme	Hydrogel	921:928	arg1	peak					891:894	a degradation peak	877:894	a degradation peak of approximately 250 °C. Hydrogel	877:928	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	11	14	theme	ALP	1258:1260	arg1	activity					1262:1269	Increased ALP activity	1248:1269	Increased ALP activity	1248:1269	Increased ALP activity was observed in MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel.					
34743940	12	15	theme	dental	1433:1438	arg1	material					1452:1459	dental scaffolding material	1433:1459	dental scaffolding material	1433:1459	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	3	16	theme	injectable	477:486	arg1	scaffold					526:533	scaffold	526:533	scaffold for bone regeneration	526:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	16	theme	injectable	477:486	arg1	hydrogel					488:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	10	17	theme	cytotoxicity	1172:1183	arg1	test					1185:1188	Primary cytotoxicity test	1164:1188	Primary cytotoxicity test	1164:1188	Primary cytotoxicity test indicated non-toxic potential of the fabricated hydrogel.					
34743940	1	18	theme	Tissue	121:126	arg1	approach					155:162	a promising approach	143:162	a promising approach to repair and regenerate damaged or lost tissues or organs	143:221	Tissue engineering is a promising approach to repair and regenerate damaged or lost tissues or organs.					
34743940	1	18	theme	Tissue	121:126	arg1	engineering					128:138	Tissue engineering	121:138	Tissue engineering	121:138	Tissue engineering is a promising approach to repair and regenerate damaged or lost tissues or organs.					
34743940	8	19	theme	swelling	957:964	arg1	ratio					966:970	a swelling ratio	955:970	a swelling ratio of around 300% at 24 h. Hydrogel	955:1003	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	8	19	theme	swelling	957:964	arg1	time					1021:1024	time	1021:1024	time passed and almost completely degraded at day 7	1021:1071	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	10	20	theme	hydrogel	1238:1245	arg1	potential					1210:1218	non-toxic potential	1200:1218	non-toxic potential of the fabricated hydrogel	1200:1245	Primary cytotoxicity test indicated non-toxic potential of the fabricated hydrogel.					
34743940	6	21	theme	synthesized	652:662	arg1	hydrogel					689:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel were characterized.					
34743940	10	22	theme	Primary	1164:1170	arg1	test					1185:1188	Primary cytotoxicity test	1164:1188	Primary cytotoxicity test	1164:1188	Primary cytotoxicity test indicated non-toxic potential of the fabricated hydrogel.					
34743940	3	23	theme	fibroin-alginate	450:465	arg1	scaffold					526:533	scaffold	526:533	scaffold for bone regeneration	526:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	23	theme	fibroin-alginate	450:465	arg1	hydrogel					488:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	24	theme	hydroxyapatite	417:430	arg1	scaffold					526:533	scaffold	526:533	scaffold for bone regeneration	526:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	24	theme	hydroxyapatite	417:430	arg1	hydrogel					488:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	5	25	theme	Bombyx	628:633	arg1	mori					635:638	Bombyx mori	628:638	Bombyx mori cocoon	628:645	Fibroin was extracted from Bombyx mori cocoon.					
34743940	3	26	theme	composite	467:475	arg1	scaffold					526:533	scaffold	526:533	scaffold for bone regeneration	526:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	26	theme	composite	467:475	arg1	hydrogel					488:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	0	27	theme	fibroin-alginate	56:71	arg1	hydrogel					83:90	fibroin-alginate composite hydrogel	56:90	fibroin-alginate composite hydrogel for bone tissue engineering	56:118	Injectable eggshell-derived hydroxyapatite-incorporated fibroin-alginate composite hydrogel for bone tissue engineering.					
34743940	13	28	theme	in	1488:1489	arg1	examinations					1496:1507	in vivo examinations	1488:1507	in vivo examinations	1488:1507	Further studies including in vivo examinations are needed prior to its clinical application.					
34743940	5	29	theme	mori	635:638	arg1	cocoon					640:645	Bombyx mori cocoon	628:645	Bombyx mori cocoon	628:645	Fibroin was extracted from Bombyx mori cocoon.					
34743940	13	30	theme	clinical	1533:1540	arg1	application					1542:1552	its clinical application	1529:1552	its clinical application	1529:1552	Further studies including in vivo examinations are needed prior to its clinical application.					
34743940	7	31	theme	pore	774:777	arg1	size					779:782	decreased pore size	764:782	decreased pore size	764:782	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	1	32	theme	promising	145:153	arg1	approach					155:162	a promising approach	143:162	a promising approach to repair and regenerate damaged or lost tissues or organs	143:221	Tissue engineering is a promising approach to repair and regenerate damaged or lost tissues or organs.					
34743940	1	32	theme	promising	145:153	arg1	engineering					128:138	Tissue engineering	121:138	Tissue engineering	121:138	Tissue engineering is a promising approach to repair and regenerate damaged or lost tissues or organs.					
34743940	7	33	contain	had	760:762	arg2	size					779:782	decreased pore size	764:782	decreased pore size	764:782	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	7	33	contain	had	760:762	arg1	hydrogel					751:758	HA-incorporated fibroin-alginate hydrogel	718:758	HA-incorporated fibroin-alginate hydrogel	718:758	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	7	33	contain	had	760:762	arg2	porosity					788:795	porosity	788:795	porosity	788:795	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	11	34	located	observed	1275:1282	arg1	MC3T3-E1					1287:1294	MC3T3-E1	1287:1294	MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel	1287:1348	Increased ALP activity was observed in MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel.					
34743940	11	34	located	observed	1275:1282	arg2	activity					1262:1269	Increased ALP activity	1248:1269	Increased ALP activity	1248:1269	Increased ALP activity was observed in MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel.					
34743940	10	35	theme	non-toxic	1200:1208	arg1	potential					1210:1218	non-toxic potential	1200:1218	non-toxic potential of the fabricated hydrogel	1200:1245	Primary cytotoxicity test indicated non-toxic potential of the fabricated hydrogel.					
34743940	11	36	theme	HA-incorporated	1308:1322	arg1	hydrogel					1341:1348	HA-incorporated fibroin-alginate hydrogel	1308:1348	HA-incorporated fibroin-alginate hydrogel	1308:1348	Increased ALP activity was observed in MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel.					
34743940	8	37	from	Hydrogel	996:1003	arg1	ratio					966:970	a swelling ratio	955:970	a swelling ratio of around 300% at 24 h. Hydrogel	955:1003	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	8	37	from	Hydrogel	996:1003	arg1	time					1021:1024	time	1021:1024	time passed and almost completely degraded at day 7	1021:1071	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	2	38	theme	resorbed	264:271	arg1	bone					282:285	the resorbed alveolar bone	260:285	the resorbed alveolar bone	260:285	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	9	39	theme	Young	1090:1094	arg1	modulus					1098:1104	Its compressive Young's modulus	1074:1104	Its compressive Young's modulus	1074:1104	Its compressive Young's modulus was approximately 0.04 ± 0.02 N/mm2 to 0.10 ± 0.02 N/mm2.					
34743940	12	40	theme	HA	1401:1402	arg1	hydrogel					1421:1428	injectable HA fibroin-alginate hydrogel	1390:1428	injectable HA fibroin-alginate hydrogel	1390:1428	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	0	41	theme	composite	73:81	arg1	hydrogel					83:90	fibroin-alginate composite hydrogel	56:90	fibroin-alginate composite hydrogel for bone tissue engineering	56:118	Injectable eggshell-derived hydroxyapatite-incorporated fibroin-alginate composite hydrogel for bone tissue engineering.					
34743940	3	42	theme	-incorporated	436:448	arg1	scaffold					526:533	scaffold	526:533	scaffold for bone regeneration	526:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	42	theme	-incorporated	436:448	arg1	hydrogel					488:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	43	used	used	518:521	arg2	hydrogel					488:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel	413:495	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	3	43	used	used	518:521	arg2	scaffold					526:533	scaffold	526:533	scaffold for bone regeneration	526:555	The hydroxyapatite (HA)-incorporated fibroin-alginate composite injectable hydrogel was fabricated to be used as scaffold for bone regeneration.					
34743940	8	44	dep	water	943:947	arg1	degraded					1009:1016	degraded	1009:1016	was degraded as time passed and almost completely degraded at day 7	1005:1071	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	12	45	theme	injectable	1390:1399	arg1	hydrogel					1421:1428	injectable HA fibroin-alginate hydrogel	1390:1428	injectable HA fibroin-alginate hydrogel	1390:1428	Results suggested the potential use of injectable HA fibroin-alginate hydrogel as dental scaffolding material.					
34743940	0	46	theme	bone	96:99	arg1	engineering					108:118	bone tissue engineering	96:118	bone tissue engineering	96:118	Injectable eggshell-derived hydroxyapatite-incorporated fibroin-alginate composite hydrogel for bone tissue engineering.					
34743940	7	47	theme	decreased	764:772	arg1	size					779:782	decreased pore size	764:782	decreased pore size	764:782	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	8	48	theme	%	985:985	arg1	ratio					966:970	a swelling ratio	955:970	a swelling ratio of around 300% at 24 h. Hydrogel	955:1003	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	8	48	theme	%	985:985	arg1	time					1021:1024	time	1021:1024	time passed and almost completely degraded at day 7	1021:1071	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	11	49	theme	fibroin-alginate	1324:1339	arg1	hydrogel					1341:1348	HA-incorporated fibroin-alginate hydrogel	1308:1348	HA-incorporated fibroin-alginate hydrogel	1308:1348	Increased ALP activity was observed in MC3T3-E1 cultured in HA-incorporated fibroin-alginate hydrogel.					
34743940	4	50	theme	eggshell	582:589	arg1	biowaste					591:598	eggshell biowaste	582:598	eggshell biowaste	582:598	HA was synthesized from eggshell biowaste.					
34743940	7	51	theme	fibroin-alginate	734:749	arg1	hydrogel					751:758	HA-incorporated fibroin-alginate hydrogel	718:758	HA-incorporated fibroin-alginate hydrogel	718:758	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	2	52	theme	substitution	359:370	arg1	success					341:347	the success	337:347	the success of dental substitution	337:370	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	8	53	dep	time	1021:1024	arg1	passed					1026:1031	passed	1026:1031	passed	1026:1031	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	8	53	dep	time	1021:1024	arg1	degraded					1055:1062	degraded	1055:1062	almost completely degraded at day 7	1037:1071	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	10	54	theme	fabricated	1227:1236	arg1	hydrogel					1238:1245	the fabricated hydrogel	1223:1245	the fabricated hydrogel	1223:1245	Primary cytotoxicity test indicated non-toxic potential of the fabricated hydrogel.					
34743940	2	55	theme	tooth	293:297	arg1	extraction					299:308	tooth extraction	293:308	tooth extraction	293:308	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	8	56	theme	24 h.	990:994	arg1	Hydrogel					996:1003	24 h. Hydrogel	990:1003	24 h. Hydrogel	990:1003	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	2	57	theme	dental	352:357	arg1	substitution					359:370	dental substitution	352:370	dental substitution	352:370	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	2	58	theme	dental	227:232	arg1	aspect					234:239	dental aspect	227:239	dental aspect	227:239	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	1	59	dep	repair	167:172	arg1	damaged					189:195	damaged	189:195	damaged	189:195	Tissue engineering is a promising approach to repair and regenerate damaged or lost tissues or organs.					
34743940	1	59	dep	repair	167:172	arg1	lost					200:203	lost	200:203	lost tissues or organs	200:221	Tissue engineering is a promising approach to repair and regenerate damaged or lost tissues or organs.					
34743940	9	60	theme	compressive	1078:1088	arg1	modulus					1098:1104	Its compressive Young's modulus	1074:1104	Its compressive Young's modulus	1074:1104	Its compressive Young's modulus was approximately 0.04 ± 0.02 N/mm2 to 0.10 ± 0.02 N/mm2.					
34743940	8	61	theme	Thermal	835:841	arg1	analysis					843:850	Thermal analysis	835:850	Thermal analysis	835:850	Thermal analysis showed that hydrogel had a degradation peak of approximately 250 °C. Hydrogel could absorb water, with a swelling ratio of around 300% at 24 h. Hydrogel was degraded as time passed and almost completely degraded at day 7.					
34743940	7	62	theme	HA-incorporated	718:732	arg1	hydrogel					751:758	HA-incorporated fibroin-alginate hydrogel	718:758	HA-incorporated fibroin-alginate hydrogel	718:758	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	2	63	theme	bone	282:285	arg1	reconstruction					242:255	reconstruction	242:255	reconstruction of the resorbed alveolar bone after tooth extraction	242:308	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	2	64	theme	implant	394:400	arg1	treatment					402:410	dental implant treatment	387:410	dental implant treatment	387:410	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	6	65	theme	alginate	680:687	arg1	hydrogel					689:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel	648:696	The synthesized HA, fibroin and alginate hydrogel were characterized.					
34743940	7	66	theme	pure	811:814	arg1	hydrogel					825:832	pure alginate hydrogel	811:832	pure alginate hydrogel	811:832	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
34743940	0	67	theme	tissue	101:106	arg1	engineering					108:118	bone tissue engineering	96:118	bone tissue engineering	96:118	Injectable eggshell-derived hydroxyapatite-incorporated fibroin-alginate composite hydrogel for bone tissue engineering.					
34743940	2	68	theme	alveolar	273:280	arg1	bone					282:285	the resorbed alveolar bone	260:285	the resorbed alveolar bone	260:285	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	2	69	theme	dental	387:392	arg1	treatment					402:410	dental implant treatment	387:410	dental implant treatment	387:410	In dental aspect, reconstruction of the resorbed alveolar bone after tooth extraction plays an important role in the success of dental substitution, especially in dental implant treatment.					
34743940	7	70	theme	alginate	816:823	arg1	hydrogel					825:832	pure alginate hydrogel	811:832	pure alginate hydrogel	811:832	HA-incorporated fibroin-alginate hydrogel had decreased pore size and porosity compared with pure alginate hydrogel.					
32942850	1	0	theme	last-line	203:211	arg1	polymyxins					234:243	polymyxins	234:243	polymyxins	234:243	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	1	0	theme	last-line	203:211	arg1	antibiotics					213:223	last-line antibiotics	203:223	last-line antibiotics	203:223	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	1	1	theme	new	284:286	arg1	antimicrobials					288:301	new antimicrobials	284:301	new antimicrobials	284:301	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	5	2	with	treatment	1032:1040	arg1	cubosomes					1047:1055	cubosomes	1047:1055	cubosomes	1047:1055	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	1	3	theme	antimicrobials	288:301	arg1	discovery					271:279	discovery	271:279	discovery of new antimicrobials	271:301	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	7	4	theme	LPS-deficient	1331:1343	arg1	pathogens					1359:1367	LPS-deficient Gram-negative pathogens	1331:1367	LPS-deficient Gram-negative pathogens	1331:1367	These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens.					
32942850	2	5	theme	significant	352:362	arg1	importance					364:373	significant importance	352:373	significant importance	352:373	Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade.					
32942850	6	6	from	membranes	1227:1235	arg1	support					1248:1254	a solid support	1240:1254	a solid support	1240:1254	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	7	7	theme	Gram-negative	1345:1357	arg1	pathogens					1359:1367	LPS-deficient Gram-negative pathogens	1331:1367	LPS-deficient Gram-negative pathogens	1331:1367	These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens.					
32942850	5	8	from	changes	933:939	arg1	membranes					955:963	biomimetic membranes	944:963	biomimetic membranes	944:963	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	6	9	theme	neutron	1127:1133	arg1	NR					1149:1150	NR	1149:1150	NR	1149:1150	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	6	9	theme	neutron	1127:1133	arg1	reflectivity					1135:1146	neutron reflectivity	1127:1146	neutron reflectivity (NR)	1127:1151	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	4	10	theme	A.	831:832	arg1	strains					844:850	polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains	775:850	polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains	775:850	Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains.					
32942850	5	11	theme	Small-angle	853:863	arg1	SANS					885:888	SANS	885:888	SANS	885:888	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	5	11	theme	Small-angle	853:863	arg1	scattering					873:882	Small-angle neutron scattering	853:882	Small-angle neutron scattering (SANS)	853:889	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	2	12	theme	MDR	416:418	arg1	emergence					403:411	the catastrophic emergence	386:411	the catastrophic emergence of MDR	386:418	Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade.					
32942850	6	13	theme	bacterial	1217:1225	arg1	membranes					1227:1235	model bacterial membranes	1211:1235	model bacterial membranes on a solid support	1211:1254	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	5	14	theme	neutron	865:871	arg1	SANS					885:888	SANS	885:888	SANS	885:888	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	5	14	theme	neutron	865:871	arg1	scattering					873:882	Small-angle neutron scattering	853:882	Small-angle neutron scattering (SANS)	853:889	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	3	15	theme	time-kill	589:597	arg1	assays					599:604	time-kill assays	589:604	time-kill assays against Gram-negative bacteria	589:635	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	6	16	theme	model	1211:1215	arg1	membranes					1227:1235	model bacterial membranes	1211:1235	model bacterial membranes on a solid support	1211:1254	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	0	17	theme	Cubosome	18:25	arg1	Formulation					27:37	Phytantriol-Based Cubosome Formulation	0:37	Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria.	0:118	Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria.					
32942850	6	18	used	used	1157:1160	arg2	NR					1149:1150	NR	1149:1150	NR	1149:1150	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	6	18	used	used	1157:1160	arg2	reflectivity					1135:1146	neutron reflectivity	1127:1146	neutron reflectivity (NR)	1127:1151	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	7	19	theme	new	1304:1306	arg1	cubosomes					1283:1291	cubosomes	1283:1291	cubosomes	1283:1291	These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens.					
32942850	7	19	theme	new	1304:1306	arg1	strategy					1308:1315	a new strategy	1302:1315	a new strategy for combating LPS-deficient Gram-negative pathogens	1302:1367	These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens.					
32942850	4	20	theme	polymyxin-resistant	775:793	arg1	strains					844:850	polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains	775:850	polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains	775:850	Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains.					
32942850	0	21	theme	Phytantriol-Based	0:16	arg1	Formulation					27:37	Phytantriol-Based Cubosome Formulation	0:37	Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria.	0:118	Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria.					
32942850	1	22	theme	multidrug-resistant	133:151	arg1	infections					169:178	multidrug-resistant (MDR) bacterial infections	133:178	multidrug-resistant (MDR) bacterial infections	133:178	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	1	22	theme	multidrug-resistant	133:151	arg1	MDR					154:156	MDR	154:156	MDR	154:156	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	6	23	theme	cubosomes	1196:1204	arg1	interaction					1181:1191	the interaction	1177:1191	the interaction of cubosomes with model bacterial membranes on a solid support	1177:1254	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	5	24	theme	structural	922:931	arg1	changes					933:939	the structural changes	918:939	the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes	918:1055	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	3	25	theme	Gram-negative	614:626	arg1	bacteria					628:635	Gram-negative bacteria	614:635	Gram-negative bacteria	614:635	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	4	26	theme	Phytantriol-based	714:730	arg1	cubosomes					732:740	Phytantriol-based cubosomes	714:740	Phytantriol-based cubosomes	714:740	Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains.					
32942850	4	27	dep	A.	831:832	arg1	baumannii					834:842	baumannii	834:842	baumannii	834:842	Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains.					
32942850	6	28	theme	membrane-cubosome	1098:1114	arg1	interface					1116:1124	the membrane-cubosome interface	1094:1124	the membrane-cubosome interface	1094:1124	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	5	29	theme	LPS-deficient	1005:1017	arg1	strains					1019:1025	these LPS-deficient strains	999:1025	these LPS-deficient strains	999:1025	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	3	30	theme	inhibitory	554:563	arg1	MIC					580:582	MIC	580:582	MIC	580:582	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	3	30	theme	inhibitory	554:563	arg1	concentration					565:577	minimum inhibitory concentration	546:577	minimum inhibitory concentration (MIC)	546:583	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	1	31	theme	urgent	255:260	arg1	need					262:265	the urgent need	251:265	the urgent need for discovery of new antimicrobials	251:301	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	0	32	theme	Lipopolysaccharide-Deficient	67:94	arg1	Bacteria					110:117	Lipopolysaccharide-Deficient Gram-Negative Bacteria	67:117	Lipopolysaccharide-Deficient Gram-Negative Bacteria	67:117	Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria.					
32942850	6	33	theme	solid	1242:1246	arg1	support					1248:1254	a solid support	1240:1254	a solid support	1240:1254	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	3	34	theme	minimum	546:552	arg1	MIC					580:582	MIC	580:582	MIC	580:582	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	3	34	theme	minimum	546:552	arg1	concentration					565:577	minimum inhibitory concentration	546:577	minimum inhibitory concentration (MIC)	546:583	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	3	35	theme	phytantriol-based	457:473	arg1	nanoparticles					475:487	phytantriol-based nanoparticles	457:487	phytantriol-based nanoparticles	457:487	In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.					
32942850	1	36	with	polymyxins	234:243	arg1	need					262:265	the urgent need	251:265	the urgent need for discovery of new antimicrobials	251:301	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	1	37	theme	bacterial	159:167	arg1	infections					169:178	multidrug-resistant (MDR) bacterial infections	133:178	multidrug-resistant (MDR) bacterial infections	133:178	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	1	37	theme	bacterial	159:167	arg1	MDR					154:156	MDR	154:156	MDR	154:156	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	4	38	dep	polymyxin-resistant	775:793	arg1	-deficient					820:829	-deficient	820:829	-deficient	820:829	Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains.					
32942850	2	39	theme	Nanotechnology-based	304:323	arg1	antimicrobials					325:338	Nanotechnology-based antimicrobials	304:338	Nanotechnology-based antimicrobials	304:338	Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade.					
32942850	1	40	theme	infections	169:178	arg1	Treatment					120:128	Treatment	120:128	Treatment of multidrug-resistant (MDR) bacterial infections	120:178	Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials.					
32942850	2	41	theme	catastrophic	390:401	arg1	emergence					403:411	the catastrophic emergence	386:411	the catastrophic emergence of MDR	386:418	Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade.					
32942850	6	42	with	interaction	1181:1191	arg1	membranes					1227:1235	model bacterial membranes	1211:1235	model bacterial membranes on a solid support	1211:1254	Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support.					
32942850	5	43	theme	biomimetic	944:953	arg1	membranes					955:963	biomimetic membranes	944:963	biomimetic membranes	944:963	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	5	44	theme	strains	1019:1025	arg1	composition					984:994	the composition	980:994	the composition of these LPS-deficient strains upon treatment with cubosomes	980:1055	Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes.					
32942850	0	45	theme	Gram-Negative	96:108	arg1	Bacteria					110:117	Lipopolysaccharide-Deficient Gram-Negative Bacteria	67:117	Lipopolysaccharide-Deficient Gram-Negative Bacteria	67:117	Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria.					
32942850	2	46	theme	past	429:432	arg1	decade					434:439	the past decade	425:439	the past decade	425:439	Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade.					
33351585	0	0	theme	Thermal	99:105	arg1	Actuators					107:115	Thermal Actuators	99:115	Thermal Actuators	99:115	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	5	1	theme	excellent	900:908	arg1	detection					923:931	excellent human motion detection	900:931	excellent human motion detection	900:931	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	4	2	theme	stable	828:833	arg1	conductivity					835:846	stable conductivity	828:846	stable conductivity	828:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	1	3	theme	thermoresponsive	182:197	arg1	hydrogels					199:207	thermoresponsive hydrogels	182:207	thermoresponsive hydrogels	182:207	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	4	4	theme	multistimulus-responsive	669:692	arg1	system					723:728	a multistimulus-responsive and multifunctional hydrogel system	667:728	a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity	667:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	4	theme	multistimulus-responsive	669:692	arg1	acrylic-acrylamide					760:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	5	5	theme	human	910:914	arg1	detection					923:931	excellent human motion detection	900:931	excellent human motion detection	900:931	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	6	6	theme	conductive	1064:1073	arg1	hydrogel					1075:1082	a conductive hydrogel	1062:1082	a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1062:1152	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	6	7	theme	actuators	1235:1243	arg1	bilayer					1195:1201	the prepared bilayer	1182:1201	the prepared bilayer	1182:1201	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	6	7	theme	actuators	1235:1243	arg1	kinds					1226:1230	two kinds	1222:1230	two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli	1222:1332	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	8	8	theme	prepared	1550:1557	arg1	system					1568:1573	the prepared hydrogel system	1546:1573	the prepared hydrogel system	1546:1573	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	7	9	theme	thermochromic	1362:1374	arg1	properties					1376:1385	the different thermochromic properties	1348:1385	the different thermochromic properties of each layer in the bilayer hydrogel	1348:1423	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	4	10	theme	multifunctional	698:712	arg1	system					723:728	a multistimulus-responsive and multifunctional hydrogel system	667:728	a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity	667:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	10	theme	multifunctional	698:712	arg1	acrylic-acrylamide					760:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	5	11	theme	prepared	866:873	arg1	hydrogel					875:882	The prepared hydrogel	862:882	The prepared hydrogel	862:882	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	2	12	from	studies	380:386	arg1	hydrogels					411:419	stimulus-responsive hydrogels	391:419	stimulus-responsive hydrogels	391:419	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	2	13	theme	stimulus-responsive	391:409	arg1	hydrogels					411:419	stimulus-responsive hydrogels	391:419	stimulus-responsive hydrogels	391:419	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	1	14	theme	wearable	309:316	arg1	devices					318:324	wearable devices	309:324	wearable devices	309:324	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	5	15	theme	signal	951:956	arg1	response					958:965	physiological signal response	937:965	physiological signal response	937:965	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	6	16	theme	thermal	1318:1324	arg1	stimuli					1326:1332	different thermal stimuli	1308:1332	different thermal stimuli	1308:1332	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	5	17	theme	environmental	1012:1024	arg1	changes					1038:1044	environmental temperature changes	1012:1044	environmental temperature changes	1012:1044	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	8	18	from	material	1610:1617	arg1	robotics					1694:1701	soft robotics	1689:1701	soft robotics	1689:1701	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	18	from	material	1610:1617	arg1	info-window					1672:1682	smart info-window	1666:1682	smart info-window	1666:1682	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	18	from	material	1610:1617	arg1	skin					1660:1663	ionic skin	1654:1663	ionic skin	1654:1663	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	18	from	material	1610:1617	arg1	applications					1632:1643	different applications	1622:1643	different applications	1622:1643	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	7	19	with	endowed	1425:1431	arg1	feature					1478:1484	a thermoresponsive "smart" feature	1451:1484	a thermoresponsive "smart" feature	1451:1484	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	6	20	theme	different	1308:1316	arg1	stimuli					1326:1332	different thermal stimuli	1308:1332	different thermal stimuli	1308:1332	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	4	21	theme	good	785:788	arg1	elasticity					790:799	good elasticity	785:799	good elasticity	785:799	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	2	22	theme	multistimulus-responsive	508:531	arg1	properties					550:559	multistimulus-responsive or multifunction properties	508:559	properties	550:559	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	8	23	theme	smart	1666:1670	arg1	info-window					1672:1682	smart info-window	1666:1682	smart info-window	1666:1682	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	4	24	theme	superior	802:809	arg1	flexibility					811:821	superior flexibility	802:821	superior flexibility	802:821	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	0	25	theme	Multiple-Stimuli-Responsive	0:26	arg1	Hydrogel					59:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel	0:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.	0:116	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	6	26	theme	shrinking	1292:1300	arg1	degrees					1268:1274	the different degrees	1254:1274	the different degrees of swelling and shrinking	1254:1300	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	5	27	theme	temperature	1026:1036	arg1	changes					1038:1044	environmental temperature changes	1012:1044	environmental temperature changes	1012:1044	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	0	28	theme	Conductive	42:51	arg1	Hydrogel					59:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel	0:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.	0:116	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	5	29	contain	possessed	976:984	arg1	hydrogel					875:882	The prepared hydrogel	862:882	The prepared hydrogel	862:882	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	5	29	contain	possessed	976:984	arg2	ability					990:996	the ability to respond to environmental temperature changes	986:1044	the ability to respond to environmental temperature changes	986:1044	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	1	30	theme	Stimulus-responsive	118:136	arg1	hydrogels					199:207	thermoresponsive hydrogels	182:207	thermoresponsive hydrogels	182:207	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	1	30	theme	Stimulus-responsive	118:136	arg1	hydrogels					168:176	conductive hydrogels	157:176	conductive hydrogels	157:176	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	1	30	theme	Stimulus-responsive	118:136	arg1	hydrogels					138:146	Stimulus-responsive hydrogels	118:146	Stimulus-responsive hydrogels	118:146	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	7	31	theme	thermoresponsive	1453:1468	arg1	feature					1478:1484	a thermoresponsive "smart" feature	1451:1484	a thermoresponsive "smart" feature	1451:1484	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	0	32	theme	Cellulose	32:40	arg1	Hydrogel					59:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel	0:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.	0:116	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	1	33	theme	artificial	330:339	arg1	muscles					341:347	artificial muscles	330:347	artificial muscles	330:347	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	6	34	theme	swelling	1279:1286	arg1	degrees					1268:1274	the different degrees	1254:1274	the different degrees of swelling and shrinking	1254:1300	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	4	35	theme	cellulose/poly	745:758	arg1	system					723:728	a multistimulus-responsive and multifunctional hydrogel system	667:728	a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity	667:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	35	theme	cellulose/poly	745:758	arg1	acrylic-acrylamide					760:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	5	36	theme	motion	916:921	arg1	detection					923:931	excellent human motion detection	900:931	excellent human motion detection	900:931	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	8	37	theme	soft	1689:1692	arg1	robotics					1694:1701	soft robotics	1689:1701	soft robotics	1689:1701	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	6	38	theme	prepared	1186:1193	arg1	bilayer					1195:1201	the prepared bilayer	1182:1201	the prepared bilayer	1182:1201	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	6	38	theme	prepared	1186:1193	arg1	kinds					1226:1230	two kinds	1222:1230	two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli	1222:1332	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	7	39	theme	"	1476:1476	arg1	feature					1478:1484	a thermoresponsive "smart" feature	1451:1484	a thermoresponsive "smart" feature	1451:1484	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	2	40	theme	stimulus-responsive	457:475	arg1	property					477:484	their single stimulus-responsive property	444:484	their single stimulus-responsive property	444:484	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	0	41	theme	Ionic	53:57	arg1	Hydrogel					59:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel	0:66	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.	0:116	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	8	42	theme	smart	1604:1608	arg1	prospects					1589:1597	excellent prospects	1579:1597	excellent prospects	1579:1597	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	42	theme	smart	1604:1608	arg1	material					1610:1617	a smart material	1602:1617	a smart material in different applications, such as ionic skin, smart info-window, and soft robotics	1602:1701	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	6	43	theme	bilayer	1164:1170	arg1	hydrogel					1172:1179	a bilayer hydrogel	1162:1179	a bilayer hydrogel	1162:1179	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	2	44	theme	single	450:455	arg1	property					477:484	their single stimulus-responsive property	444:484	their single stimulus-responsive property	444:484	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	0	45	theme	Smart	72:76	arg1	Devices					87:93	Smart Wearable Devices	72:93	Smart Wearable Devices	72:93	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	6	46	theme	poly	1108:1111	arg1	hydrogel					1145:1152	a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1089:1152	a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1089:1152	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	0	47	theme	Wearable	78:85	arg1	Devices					87:93	Smart Wearable Devices	72:93	Smart Wearable Devices	72:93	Multiple-Stimuli-Responsive and Cellulose Conductive Ionic Hydrogel for Smart Wearable Devices and Thermal Actuators.					
33351585	4	48	with	system	723:728	arg1	elasticity					790:799	good elasticity	785:799	good elasticity	785:799	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	48	with	system	723:728	arg1	flexibility					811:821	superior flexibility	802:821	superior flexibility	802:821	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	48	with	system	723:728	arg1	conductivity					835:846	stable conductivity	828:846	stable conductivity	828:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	5	49	theme	physiological	937:949	arg1	response					958:965	physiological signal response	937:965	physiological signal response	937:965	The prepared hydrogel not only showed excellent human motion detection and physiological signal response but also possessed the ability to respond to environmental temperature changes.					
33351585	6	50	theme	thermoresponsive	1091:1106	arg1	hydrogel					1145:1152	a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1089:1152	a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1089:1152	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	7	51	theme	different	1352:1360	arg1	properties					1376:1385	the different thermochromic properties	1348:1385	the different thermochromic properties of each layer in the bilayer hydrogel	1348:1423	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	2	52	theme	existing	371:378	arg1	studies					380:386	the existing studies	367:386	the existing studies on stimulus-responsive hydrogels	367:419	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	7	53	theme	bilayer	1408:1414	arg1	hydrogel					1416:1423	the bilayer hydrogel	1404:1423	the bilayer hydrogel	1404:1423	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	7	54	from	properties	1376:1385	arg1	hydrogel					1416:1423	the bilayer hydrogel	1404:1423	the bilayer hydrogel	1404:1423	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	6	55	theme	different	1258:1266	arg1	degrees					1268:1274	the different degrees	1254:1274	the different degrees of swelling and shrinking	1254:1300	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	1	56	theme	promising	260:268	arg1	candidates					270:279	promising candidates	260:279	promising candidates for smart materials such as wearable devices and artificial muscles	260:347	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	8	57	theme	hydrogel	1559:1566	arg1	system					1568:1573	the prepared hydrogel system	1546:1573	the prepared hydrogel system	1546:1573	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	1	58	theme	conductive	157:166	arg1	hydrogels					168:176	conductive hydrogels	157:176	conductive hydrogels	157:176	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	7	59	theme	smart	1471:1475	arg1	feature					1478:1484	a thermoresponsive "smart" feature	1451:1484	a thermoresponsive "smart" feature	1451:1484	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	4	60	theme	hydrogel	714:721	arg1	system					723:728	a multistimulus-responsive and multifunctional hydrogel system	667:728	a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity	667:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	60	theme	hydrogel	714:721	arg1	acrylic-acrylamide					760:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	8	61	theme	ionic	1654:1658	arg1	skin					1660:1663	ionic skin	1654:1663	ionic skin	1654:1663	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	62	theme	different	1622:1630	arg1	robotics					1694:1701	soft robotics	1689:1701	soft robotics	1689:1701	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	62	theme	different	1622:1630	arg1	info-window					1672:1682	smart info-window	1666:1682	smart info-window	1666:1682	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	62	theme	different	1622:1630	arg1	skin					1660:1663	ionic skin	1654:1663	ionic skin	1654:1663	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	62	theme	different	1622:1630	arg1	applications					1632:1643	different applications	1622:1643	different applications	1622:1643	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	63	theme	excellent	1579:1587	arg1	prospects					1589:1597	excellent prospects	1579:1597	excellent prospects	1579:1597	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	63	theme	excellent	1579:1587	arg1	material					1610:1617	a smart material	1602:1617	a smart material in different applications, such as ionic skin, smart info-window, and soft robotics	1602:1701	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	6	64	with	hydrogel	1075:1082	arg1	hydrogel					1145:1152	a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1089:1152	a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel	1089:1152	By integrating a conductive hydrogel with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) hydrogel to form a bilayer hydrogel, the prepared bilayer also functioned as two kinds of actuators owing to the different degrees of swelling and shrinking under different thermal stimuli.					
33351585	8	65	contain	has	1575:1577	arg1	system					1568:1573	the prepared hydrogel system	1546:1573	the prepared hydrogel system	1546:1573	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	65	contain	has	1575:1577	arg2	material					1610:1617	a smart material	1602:1617	a smart material in different applications, such as ionic skin, smart info-window, and soft robotics	1602:1701	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	8	65	contain	has	1575:1577	arg2	prospects					1589:1597	excellent prospects	1579:1597	excellent prospects	1579:1597	Therefore, the prepared hydrogel system has excellent prospects as a smart material in different applications, such as ionic skin, smart info-window, and soft robotics.					
33351585	4	66	theme	carboxymethyl	731:743	arg1	system					723:728	a multistimulus-responsive and multifunctional hydrogel system	667:728	a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity	667:846	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	4	66	theme	carboxymethyl	731:743	arg1	acrylic-acrylamide					760:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	carboxymethyl cellulose/poly acrylic-acrylamide	731:777	In this work, a multistimulus-responsive and multifunctional hydrogel system (carboxymethyl cellulose/poly acrylic-acrylamide) with good elasticity, superior flexibility, and stable conductivity was prepared.					
33351585	2	67	theme	multifunction	536:548	arg1	properties					550:559	multistimulus-responsive or multifunction properties	508:559	properties	550:559	However, most of the existing studies on stimulus-responsive hydrogels have mainly focused on their single stimulus-responsive property and have not explored multistimulus-responsive or multifunction properties.					
33351585	3	68	theme	prepared	615:622	arg1	incompatible					639:650	incompatible	639:650	incompatible	639:650	Although some works involved multifunctionality, the prepared hydrogels were incompatible.					
33351585	3	68	theme	prepared	615:622	arg1	hydrogels					624:632	the prepared hydrogels	611:632	the prepared hydrogels	611:632	Although some works involved multifunctionality, the prepared hydrogels were incompatible.					
33351585	7	69	theme	layer	1395:1399	arg1	properties					1376:1385	the different thermochromic properties	1348:1385	the different thermochromic properties of each layer in the bilayer hydrogel	1348:1423	Furthermore, the different thermochromic properties of each layer in the bilayer hydrogel endowed the hydrogel with a thermoresponsive "smart" feature, the ability to display and conceal information.					
33351585	1	70	theme	smart	285:289	arg1	devices					318:324	wearable devices	309:324	wearable devices	309:324	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	1	70	theme	smart	285:289	arg1	muscles					341:347	artificial muscles	330:347	artificial muscles	330:347	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
33351585	1	70	theme	smart	285:289	arg1	materials					291:299	smart materials	285:299	smart materials such as wearable devices and artificial muscles	285:347	Stimulus-responsive hydrogels, such as conductive hydrogels and thermoresponsive hydrogels, have been explored extensively and are considered promising candidates for smart materials such as wearable devices and artificial muscles.					
34482977	7	0	theme	starch	1218:1223	arg1	level					1204:1208	each level	1199:1208	each level of corn starch infused into the abomasum	1199:1249	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	4	1	theme	starch/d	750:757	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	1	theme	starch/d	750:757	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	1	theme	starch/d	750:757	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	1	theme	starch/d	750:757	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	1	theme	starch/d	750:757	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	2	theme	starch/d	859:866	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	2	theme	starch/d	859:866	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	2	theme	starch/d	859:866	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	2	theme	starch/d	859:866	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	2	theme	starch/d	859:866	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	12	3	theme	BHB	2200:2202	arg1	concentrations					2182:2195	increased concentrations	2172:2195	increased concentrations of BHB in milk and plasma	2172:2221	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	2	4	theme	infusion	535:542	arg1	d					510:510	5 d	508:510	5 d of continuous abomasal infusion treatments followed by 2 d of rest	508:577	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	9	5	theme	hindgut	1578:1584	arg1	acidosis					1586:1593	This induced hindgut acidosis	1565:1593	This induced hindgut acidosis	1565:1593	This induced hindgut acidosis was associated with decreased digestibility of nutrients, except for crude fat and NDF, which were not affected.					
34482977	8	6	theme	acidic	1546:1551	arg1	conditions					1553:1562	the more acidic conditions	1537:1562	the more acidic conditions	1537:1562	No systemic inflammatory response was observed and the permeability of the intestine or hindgut epithelium was not affected by the more acidic conditions.					
34482977	13	7	theme	compensated	2316:2326	arg1	acidosis					2338:2345	Induced compensated metabolic acidosis	2308:2345	Induced compensated metabolic acidosis	2308:2345	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	5	8	theme	%	967:967	arg1	concentrate					969:979	27.6% concentrate	963:979	27.6% concentrate (on a dry matter basis)	963:1003	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	11	9	dep	CO2	2000:2002	arg1	i.e.					1972:1975	i.e.	1972:1975	i.e.	1972:1975	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	10	10	theme	energy	1784:1789	arg1	balance					1791:1797	energy balance	1784:1797	energy balance	1784:1797	Induced hindgut acidosis did not affect milk production and composition and energy balance, but increased milk N efficiency.					
34482977	3	11	theme	corn	665:668	arg1	starch					670:675	corn starch	665:675	corn starch	665:675	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	12	12	from	plasma	2216:2221	arg1	concentrations					2182:2195	increased concentrations	2172:2195	increased concentrations of BHB in milk and plasma	2172:2221	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	10	13	theme	N	1819:1819	arg1	efficiency					1821:1830	milk N efficiency	1814:1830	milk N efficiency	1814:1830	Induced hindgut acidosis did not affect milk production and composition and energy balance, but increased milk N efficiency.					
34482977	0	14	theme	metabolic	125:133	arg1	acidosis					135:142	metabolic acidosis	125:142	metabolic acidosis	125:142	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	1	15	theme	dairy	378:382	arg1	cows					384:387	early-lactation dairy cows	362:387	early-lactation dairy cows	362:387	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	8	16	theme	inflammatory	1422:1433	arg1	response					1435:1442	No systemic inflammatory response	1410:1442	No systemic inflammatory response	1410:1442	No systemic inflammatory response was observed and the permeability of the intestine or hindgut epithelium was not affected by the more acidic conditions.					
34482977	1	17	theme	metabolic	201:209	arg1	acidosis					211:218	metabolic acidosis	201:218	metabolic acidosis	201:218	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	2	18	dep	infusion	535:542	arg1	treatments					544:553	treatments	544:553	continuous abomasal infusion treatments followed by 2 d of rest	515:577	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	7	19	theme	corn	1309:1312	arg1	starch/d					1314:1321	corn starch/d	1309:1321	corn starch/d	1309:1321	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	9	20	theme	induced	1570:1576	arg1	acidosis					1586:1593	This induced hindgut acidosis	1565:1593	This induced hindgut acidosis	1565:1593	This induced hindgut acidosis was associated with decreased digestibility of nutrients, except for crude fat and NDF, which were not affected.					
34482977	9	21	theme	nutrients	1642:1650	arg1	digestibility					1625:1637	decreased digestibility	1615:1637	decreased digestibility of nutrients	1615:1650	This induced hindgut acidosis was associated with decreased digestibility of nutrients, except for crude fat and NDF, which were not affected.					
34482977	5	22	from	concentrate	969:979	arg1	basis					998:1002	a dry matter basis	985:1002	a dry matter basis	985:1002	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	2	23	theme	square	407:412	arg1	design					414:419	a 6 × 6 Latin square design	393:419	a 6 × 6 Latin square design	393:419	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	12	24	from	BHB	2200:2202	arg1	milk					2207:2210	milk	2207:2210	milk	2207:2210	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	12	24	from	BHB	2200:2202	arg1	plasma					2216:2221	plasma	2216:2221	plasma	2216:2221	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	2	25	theme	continuous	515:524	arg1	infusion					535:542	continuous abomasal infusion treatments	515:553	continuous abomasal infusion treatments followed by 2 d of rest	515:577	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	12	26	theme	critical	2257:2264	arg1	values					2276:2281	the critical threshold values	2253:2281	the critical threshold values for subclinical ketosis	2253:2305	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	4	27	theme	starch/d	775:782	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	27	theme	starch/d	775:782	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	27	theme	starch/d	775:782	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	27	theme	starch/d	775:782	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	27	theme	starch/d	775:782	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	9	28	theme	crude	1664:1668	arg1	fat					1670:1672	crude fat	1664:1672	crude fat	1664:1672	This induced hindgut acidosis was associated with decreased digestibility of nutrients, except for crude fat and NDF, which were not affected.					
34482977	12	29	theme	increased	2172:2180	arg1	concentrations					2182:2195	increased concentrations	2172:2195	increased concentrations of BHB in milk and plasma	2172:2221	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	11	30	theme	urinary	2057:2063	arg1	pH					2065:2066	decreased urinary pH	2047:2066	decreased urinary pH	2047:2066	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	12	31	from	milk	2207:2210	arg1	concentrations					2182:2195	increased concentrations	2172:2195	increased concentrations of BHB in milk and plasma	2172:2221	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	4	32	theme	starch/d	815:822	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	32	theme	starch/d	815:822	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	32	theme	starch/d	815:822	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	32	theme	starch/d	815:822	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	32	theme	starch/d	815:822	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	12	33	theme	BHB	2156:2158	arg1	infusion					2144:2151	Abomasal infusion	2135:2151	Abomasal infusion of BHB	2135:2158	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	5	34	theme	ad	1023:1024	arg1	intake					1034:1039	ad libitum intake	1023:1039	ad libitum intake of individual cows	1023:1058	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	3	35	theme	BHB	693:695	arg1	levels					655:660	3 levels	653:660	3 levels of corn starch	653:675	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	3	35	theme	BHB	693:695	arg1	levels					683:688	2 levels	681:688	2 levels of BHB	681:695	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	0	36	theme	early-lactation	58:72	arg1	cows					98:101	early-lactation Holstein-Friesian dairy cows	58:101	early-lactation Holstein-Friesian dairy cows	58:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	5	37	theme	35.0	920:923	arg1	%					924:924	%	924:924	%	924:924	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	11	38	theme	Abomasal	1833:1840	arg1	infusion					1842:1849	Abomasal infusion	1833:1849	Abomasal infusion of BHB	1833:1856	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	13	39	theme	milk	2441:2444	arg1	production					2446:2455	milk production	2441:2455	milk production	2441:2455	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	7	40	theme	starch	1393:1398	arg1	infusion					1400:1407	corn starch infusion	1388:1407	corn starch infusion	1388:1407	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	11	41	theme	compensated	1872:1882	arg1	acidosis					1894:1901	a compensated metabolic acidosis	1870:1901	a compensated metabolic acidosis	1870:1901	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	0	42	theme	dairy	92:96	arg1	cows					98:101	early-lactation Holstein-Friesian dairy cows	58:101	early-lactation Holstein-Friesian dairy cows	58:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	5	43	theme	grass	926:930	arg1	silage					932:937	35.0% grass silage	920:937	35.0% grass silage	920:937	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	13	44	theme	body	2416:2419	arg1	fat					2421:2423	body fat	2416:2423	body fat	2416:2423	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	1	45	theme	study	168:172	arg1	objectives					149:158	The objectives	145:158	The objectives of this study	145:172	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	10	46	theme	Induced	1708:1714	arg1	acidosis					1724:1731	Induced hindgut acidosis	1708:1731	Induced hindgut acidosis	1708:1731	Induced hindgut acidosis did not affect milk production and composition and energy balance, but increased milk N efficiency.					
34482977	5	47	theme	mixed	893:897	arg1	ration					899:904	A total mixed ration	885:904	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis)	885:1003	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	8	48	theme	epithelium	1506:1515	arg1	permeability					1465:1476	the permeability	1461:1476	the permeability of the intestine or hindgut epithelium	1461:1515	No systemic inflammatory response was observed and the permeability of the intestine or hindgut epithelium was not affected by the more acidic conditions.					
34482977	0	49	from	β-hydroxybutyrate	37:53	arg1	cows					98:101	early-lactation Holstein-Friesian dairy cows	58:101	early-lactation Holstein-Friesian dairy cows	58:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	2	50	theme	rest	574:577	arg1	d					569:569	2 d	567:569	2 d of rest	567:577	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	2	51	theme	dairy	459:463	arg1	cows					465:468	6 rumen-fistulated Holstein-Friesian dairy cows	422:468	6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk)	422:488	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	7	52	theme	hindgut	1354:1360	arg1	acidosis					1362:1369	hindgut acidosis	1354:1369	hindgut acidosis	1354:1369	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	0	53	theme	starch	26:31	arg1	infusion					9:16	Abomasal infusion	0:16	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows	0:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	0	54	from	starch	26:31	arg1	cows					98:101	early-lactation Holstein-Friesian dairy cows	58:101	early-lactation Holstein-Friesian dairy cows	58:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	2	55	theme	±	474:474	arg1	d					479:479	66 ± 18 d	471:479	66 ± 18 d in milk	471:487	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	0	56	theme	Abomasal	0:7	arg1	infusion					9:16	Abomasal infusion	0:16	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows	0:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	4	57	theme	corn	854:857	arg1	starch/d					859:866	corn starch/d	854:866	corn starch/d	854:866	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	5	58	theme	intake	1034:1039	arg1	%					1018:1018	90%	1016:1018	90% of ad libitum intake of individual cows	1016:1058	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	5	58	theme	intake	1034:1039	arg1	intake					1034:1039	ad libitum intake	1023:1039	ad libitum intake of individual cows	1023:1058	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	6	59	theme	N	1164:1164	arg1	balance					1166:1172	N balance	1164:1172	N balance	1164:1172	The experiment was conducted in climate respiration chambers to facilitate determination of energy and N balance.					
34482977	4	60	theme	BHB/d	834:838	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	60	theme	BHB/d	834:838	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	60	theme	BHB/d	834:838	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	60	theme	BHB/d	834:838	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	60	theme	BHB/d	834:838	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	3	61	theme	abomasal	584:591	arg1	treatments					602:611	The abomasal infusion treatments	580:611	The abomasal infusion treatments	580:611	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	5	62	theme	individual	1044:1053	arg1	cows					1055:1058	individual cows	1044:1058	individual cows	1044:1058	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	6	63	theme	energy	1153:1158	arg1	determination					1136:1148	determination	1136:1148	determination of energy and N balance	1136:1172	The experiment was conducted in climate respiration chambers to facilitate determination of energy and N balance.					
34482977	5	64	from	silage	932:937	arg1	basis					998:1002	a dry matter basis	985:1002	a dry matter basis	985:1002	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	12	65	from	concentrations	2182:2195	arg1	milk					2207:2210	milk	2207:2210	milk	2207:2210	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	12	65	from	concentrations	2182:2195	arg1	plasma					2216:2221	plasma	2216:2221	plasma	2216:2221	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	2	66	theme	rumen-fistulated	424:439	arg1	cows					465:468	6 rumen-fistulated Holstein-Friesian dairy cows	422:468	6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk)	422:488	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	1	67	from	effects	321:327	arg1	cows					384:387	early-lactation dairy cows	362:387	early-lactation dairy cows	362:387	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	5	68	theme	%	944:944	arg1	silage					951:956	37.4% corn silage	940:956	37.4% corn silage	940:956	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	2	69	dep	cows	465:468	arg1	d					479:479	66 ± 18 d	471:479	66 ± 18 d in milk	471:487	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	10	70	theme	milk	1748:1751	arg1	production					1753:1762	milk production	1748:1762	milk production	1748:1762	Induced hindgut acidosis did not affect milk production and composition and energy balance, but increased milk N efficiency.					
34482977	13	71	theme	infused	2374:2380	arg1	BHB					2382:2384	abomasally infused BHB	2363:2384	abomasally infused BHB	2363:2384	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	1	72	theme	abomasal	224:231	arg1	infusion					233:240	abomasal infusion	224:240	abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively	224:297	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	6	73	theme	balance	1166:1172	arg1	determination					1136:1148	determination	1136:1148	determination of energy and N balance	1136:1172	The experiment was conducted in climate respiration chambers to facilitate determination of energy and N balance.					
34482977	11	74	dep	status	1964:1969	arg1	excess					2020:2025	base excess	2015:2025	base excess	2015:2025	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	11	74	dep	status	1964:1969	arg1	HCO3					2005:2008	HCO3	2005:2008	HCO3	2005:2008	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	11	74	dep	status	1964:1969	arg1	tendency					2034:2041	a tendency	2032:2041	a tendency for decreased urinary pH	2032:2066	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	11	74	dep	status	1964:1969	arg1	CO2					2000:2002	decreased blood total CO2	1978:2002	decreased blood total CO2	1978:2002	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	5	75	theme	matter	991:996	arg1	basis					998:1002	a dry matter basis	985:1002	a dry matter basis	985:1002	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	11	76	theme	BHB	1854:1856	arg1	infusion					1842:1849	Abomasal infusion	1833:1849	Abomasal infusion of BHB	1833:1856	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	7	77	theme	corn	1213:1216	arg1	starch					1218:1223	corn starch	1213:1223	corn starch infused into the abomasum	1213:1249	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	3	78	theme	factorial	630:638	arg1	design					640:645	a 3 × 2 factorial design	622:645	a 3 × 2 factorial design	622:645	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	1	79	theme	starch	250:255	arg1	infusion					233:240	abomasal infusion	224:240	abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively	224:297	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	4	80	theme	corn	745:748	arg1	starch/d					750:757	corn starch/d	745:757	corn starch/d	745:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	81	theme	mol	874:876	arg1	BHB/d					878:882	8.0 mol BHB/d	870:882	8.0 mol BHB/d	870:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	3	82	theme	×	626:626	arg1	design					640:645	a 3 × 2 factorial design	622:645	a 3 × 2 factorial design	622:645	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	1	83	theme	β-hydroxybutyrate	261:277	arg1	infusion					233:240	abomasal infusion	224:240	abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively	224:297	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	2	84	theme	abomasal	526:533	arg1	infusion					535:542	continuous abomasal infusion treatments	515:553	continuous abomasal infusion treatments followed by 2 d of rest	515:577	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	7	85	theme	Fecal	1175:1179	arg1	pH					1181:1182	Fecal pH	1175:1182	Fecal pH	1175:1182	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	11	86	theme	clear	1933:1937	arg1	disturbance					1939:1949	a clear disturbance	1931:1949	a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH)	1931:2067	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	13	87	theme	Induced	2308:2314	arg1	acidosis					2338:2345	Induced compensated metabolic acidosis	2308:2345	Induced compensated metabolic acidosis	2308:2345	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	13	88	theme	metabolic	2328:2336	arg1	acidosis					2338:2345	Induced compensated metabolic acidosis	2308:2345	Induced compensated metabolic acidosis	2308:2345	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	0	89	theme	β-hydroxybutyrate	37:53	arg1	infusion					9:16	Abomasal infusion	0:16	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows	0:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	12	90	theme	Abomasal	2135:2142	arg1	infusion					2144:2151	Abomasal infusion	2135:2151	Abomasal infusion of BHB	2135:2158	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	6	91	theme	respiration	1101:1111	arg1	chambers					1113:1120	climate respiration chambers	1093:1120	climate respiration chambers	1093:1120	The experiment was conducted in climate respiration chambers to facilitate determination of energy and N balance.					
34482977	5	92	from	silage	951:956	arg1	basis					998:1002	a dry matter basis	985:1002	a dry matter basis	985:1002	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	7	93	theme	starch/d	1314:1321	arg1	kg					1303:1304	6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg	1259:1304	6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d	1259:1321	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	9	94	theme	decreased	1615:1623	arg1	digestibility					1625:1637	decreased digestibility	1615:1637	decreased digestibility of nutrients	1615:1650	This induced hindgut acidosis was associated with decreased digestibility of nutrients, except for crude fat and NDF, which were not affected.					
34482977	3	95	theme	starch	670:675	arg1	levels					655:660	3 levels	653:660	3 levels of corn starch	653:675	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	3	95	theme	starch	670:675	arg1	levels					683:688	2 levels	681:688	2 levels of BHB	681:695	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	0	96	from	cows	98:101	arg1	infusion					9:16	Abomasal infusion	0:16	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows	0:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	10	97	theme	milk	1814:1817	arg1	efficiency					1821:1830	milk N efficiency	1814:1830	milk N efficiency	1814:1830	Induced hindgut acidosis did not affect milk production and composition and energy balance, but increased milk N efficiency.					
34482977	8	98	theme	systemic	1413:1420	arg1	response					1435:1442	No systemic inflammatory response	1410:1442	No systemic inflammatory response	1410:1442	No systemic inflammatory response was observed and the permeability of the intestine or hindgut epithelium was not affected by the more acidic conditions.					
34482977	4	99	dep	water	717:721	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	99	dep	water	717:721	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	99	dep	water	717:721	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	99	dep	water	717:721	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	99	dep	water	717:721	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	11	100	theme	blood	1988:1992	arg1	CO2					2000:2002	decreased blood total CO2	1978:2002	decreased blood total CO2	1978:2002	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	4	101	theme	mol	789:791	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	12	102	theme	threshold	2266:2274	arg1	values					2276:2281	the critical threshold values	2253:2281	the critical threshold values for subclinical ketosis	2253:2305	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	5	103	theme	total	887:891	arg1	ration					899:904	A total mixed ration	885:904	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis)	885:1003	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	12	104	theme	subclinical	2287:2297	arg1	ketosis					2299:2305	subclinical ketosis	2287:2305	subclinical ketosis	2287:2305	Abomasal infusion of BHB resulted in increased concentrations of BHB in milk and plasma, but both remained well below the critical threshold values for subclinical ketosis.					
34482977	2	105	from	d	479:479	arg1	milk					484:487	milk	484:487	milk	484:487	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	11	106	theme	decreased	2047:2055	arg1	pH					2065:2066	decreased urinary pH	2047:2066	decreased urinary pH	2047:2066	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	13	107	theme	inflammatory	2476:2487	arg1	response					2489:2496	inflammatory response	2476:2496	inflammatory response	2476:2496	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	4	108	theme	corn	770:773	arg1	starch/d					775:782	corn starch/d	770:782	corn starch/d	770:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	11	109	theme	blood	2078:2082	arg1	pH					2084:2085	blood pH	2078:2085	blood pH	2078:2085	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	0	110	theme	Holstein-Friesian	74:90	arg1	cows					98:101	early-lactation Holstein-Friesian dairy cows	58:101	early-lactation Holstein-Friesian dairy cows	58:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	11	111	theme	total	1994:1998	arg1	CO2					2000:2002	decreased blood total CO2	1978:2002	decreased blood total CO2	1978:2002	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	4	112	theme	corn	810:813	arg1	starch/d					815:822	corn starch/d	810:822	corn starch/d	810:822	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	5	113	theme	%	924:924	arg1	silage					932:937	35.0% grass silage	920:937	35.0% grass silage	920:937	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	1	114	theme	physiological	338:350	arg1	states					352:357	these physiological states	332:357	these physiological states	332:357	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	11	115	theme	metabolic	1884:1892	arg1	acidosis					1894:1901	a compensated metabolic acidosis	1870:1901	a compensated metabolic acidosis	1870:1901	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	0	116	from	infusion	9:16	arg1	cows					98:101	early-lactation Holstein-Friesian dairy cows	58:101	early-lactation Holstein-Friesian dairy cows	58:101	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	10	117	theme	hindgut	1716:1722	arg1	acidosis					1724:1731	Induced hindgut acidosis	1708:1731	Induced hindgut acidosis	1708:1731	Induced hindgut acidosis did not affect milk production and composition and energy balance, but increased milk N efficiency.					
34482977	6	118	theme	climate	1093:1099	arg1	chambers					1113:1120	climate respiration chambers	1093:1120	climate respiration chambers	1093:1120	The experiment was conducted in climate respiration chambers to facilitate determination of energy and N balance.					
34482977	8	119	theme	intestine	1485:1493	arg1	permeability					1465:1476	the permeability	1461:1476	the permeability of the intestine or hindgut epithelium	1461:1515	No systemic inflammatory response was observed and the permeability of the intestine or hindgut epithelium was not affected by the more acidic conditions.					
34482977	7	120	theme	corn	1388:1391	arg1	infusion					1400:1407	corn starch infusion	1388:1407	corn starch infusion	1388:1407	Fecal pH decreased with each level of corn starch infused into the abomasum and was 6.49, 6.00, and 5.15 with 0.0, 1.5, and 3.0 kg of corn starch/d, respectively, suggesting that hindgut acidosis was induced with corn starch infusion.					
34482977	11	121	theme	acid-base	1954:1962	arg1	status					1964:1969	acid-base status	1954:1969	acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH)	1954:2067	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	5	122	theme	cows	1055:1058	arg1	intake					1034:1039	ad libitum intake	1023:1039	ad libitum intake of individual cows	1023:1058	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	1	123	theme	states	352:357	arg1	effects					321:327	the effects	317:327	the effects of these physiological states in early-lactation dairy cows	317:387	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	8	124	theme	hindgut	1498:1504	arg1	epithelium					1506:1515	hindgut epithelium	1498:1515	hindgut epithelium	1498:1515	No systemic inflammatory response was observed and the permeability of the intestine or hindgut epithelium was not affected by the more acidic conditions.					
34482977	11	125	theme	normal	2121:2126	arg1	range					2128:2132	a physiologically normal range	2103:2132	a physiologically normal range	2103:2132	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	11	126	dep	resulted	1858:1865	arg1	whereas					2070:2076	whereas	2070:2076	whereas	2070:2076	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	2	127	theme	Holstein-Friesian	441:457	arg1	cows					465:468	6 rumen-fistulated Holstein-Friesian dairy cows	422:468	6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk)	422:488	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	1	128	theme	early-lactation	362:376	arg1	cows					384:387	early-lactation dairy cows	362:387	early-lactation dairy cows	362:387	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34482977	5	129	theme	libitum	1026:1032	arg1	intake					1034:1039	ad libitum intake	1023:1039	ad libitum intake of individual cows	1023:1058	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	0	130	theme	corn	21:24	arg1	starch					26:31	corn starch	21:31	corn starch	21:31	Abomasal infusion of corn starch and β-hydroxybutyrate in early-lactation Holstein-Friesian dairy cows to induce hindgut and metabolic acidosis.					
34482977	11	131	theme	base	2015:2018	arg1	excess					2020:2025	base excess	2015:2025	base excess	2015:2025	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	2	132	theme	Latin	401:405	arg1	design					414:419	a 6 × 6 Latin square design	393:419	a 6 × 6 Latin square design	393:419	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	11	133	theme	status	1964:1969	arg1	disturbance					1939:1949	a clear disturbance	1931:1949	a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH)	1931:2067	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	2	134	theme	×	397:397	arg1	design					414:419	a 6 × 6 Latin square design	393:419	a 6 × 6 Latin square design	393:419	In a 6 × 6 Latin square design, 6 rumen-fistulated Holstein-Friesian dairy cows (66 ± 18 d in milk) were subjected to 5 d of continuous abomasal infusion treatments followed by 2 d of rest.					
34482977	5	135	theme	27.6	963:966	arg1	%					967:967	%	967:967	%	967:967	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	4	136	theme	mol	830:832	arg1	BHB/d					834:838	8.0 mol BHB/d	826:838	8.0 mol BHB/d	826:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	5	137	theme	37.4	940:943	arg1	%					944:944	%	944:944	%	944:944	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	13	138	theme	intestinal	2513:2522	arg1	permeability					2524:2535	intestinal permeability	2513:2535	intestinal permeability	2513:2535	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	5	139	theme	corn	946:949	arg1	silage					951:956	37.4% corn silage	940:956	37.4% corn silage	940:956	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	4	140	theme	BHB/d	878:882	arg1	kg					739:740	1.5 kg	735:740	1.5 kg of corn starch/d	735:757	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	140	theme	BHB/d	878:882	arg1	kg					804:805	1.5 kg	800:805	1.5 kg of corn starch/d + 8.0 mol BHB/d	800:838	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	140	theme	BHB/d	878:882	arg1	kg					764:765	3.0 kg	760:765	3.0 kg of corn starch/d	760:782	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	140	theme	BHB/d	878:882	arg1	kg					848:849	3.0 kg	844:849	3.0 kg of corn starch/d + 8.0 mol BHB/d	844:882	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	4	140	theme	BHB/d	878:882	arg1	BHB/d					793:797	8.0 mol BHB/d	785:797	8.0 mol BHB/d	785:797	The infusions were water as control, 1.5 kg of corn starch/d, 3.0 kg of corn starch/d, 8.0 mol BHB/d, 1.5 kg of corn starch/d + 8.0 mol BHB/d, or 3.0 kg of corn starch/d + 8.0 mol BHB/d.					
34482977	3	141	theme	infusion	593:600	arg1	treatments					602:611	The abomasal infusion treatments	580:611	The abomasal infusion treatments	580:611	The abomasal infusion treatments followed a 3 × 2 factorial design, with 3 levels of corn starch and 2 levels of BHB.					
34482977	13	142	theme	BHB	2382:2384	arg1	result					2353:2358	a result	2351:2358	a result of abomasally infused BHB	2351:2384	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	13	142	theme	BHB	2382:2384	arg1	energy					2397:2402	energy	2397:2402	energy retained as body fat	2397:2423	Induced compensated metabolic acidosis, as a result of abomasally infused BHB, increased energy retained as body fat, did not affect milk production and composition or inflammatory response, but increased intestinal permeability.					
34482977	5	143	theme	dry	987:989	arg1	basis					998:1002	a dry matter basis	985:1002	a dry matter basis	985:1002	A total mixed ration consisting of 35.0% grass silage, 37.4% corn silage, and 27.6% concentrate (on a dry matter basis) was fed at 90% of ad libitum intake of individual cows.					
34482977	11	144	theme	decreased	1978:1986	arg1	CO2					2000:2002	decreased blood total CO2	1978:2002	decreased blood total CO2	1978:2002	Abomasal infusion of BHB resulted in a compensated metabolic acidosis, which was characterized by a clear disturbance of acid-base status (i.e., decreased blood total CO2, HCO3, and base excess, and a tendency for decreased urinary pH), whereas blood pH remained within a physiologically normal range.					
34482977	1	145	theme	corn	245:248	arg1	starch					250:255	corn starch	245:255	corn starch	245:255	The objectives of this study were to induce hindgut and metabolic acidosis via abomasal infusion of corn starch and β-hydroxybutyrate (BHB), respectively, and to determine the effects of these physiological states in early-lactation dairy cows.					
34399281	3	0	theme	early	519:523	arg1	stages					525:530	early stages	519:530	early stages of cancer	519:540	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	10	1	from	presence	1457:1464	arg1	type					1511:1514	this tumor type	1500:1514	this tumor type	1500:1514	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	6	2	from	areas	947:951	arg1	CMT					963:965	CMT	963:965	CMT	963:965	VCAN was highly expressed in stroma adjacent to invasive areas in SC and CMT.					
34399281	6	2	from	areas	947:951	arg1	SC					956:957	SC	956:957	SC	956:957	VCAN was highly expressed in stroma adjacent to invasive areas in SC and CMT.					
34399281	9	3	theme	CD44	1217:1220	arg1	expression					1231:1240	increased CD44 and EGFR expression	1207:1240	increased CD44 and EGFR expression	1207:1240	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	3	4	theme	malignant	430:438	arg1	cells					451:455	malignant epithelial cells	430:455	malignant epithelial cells	430:455	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	5	from	responsible	464:474	arg1	addition					352:359	addition	352:359	addition	352:359	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	6	from	addition	352:359	arg1	responsible					464:474	responsible	464:474	responsible	464:474	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	9	7	from	areas	1264:1268	arg1	SC					1273:1274	SC	1273:1274	SC compared to CMT	1273:1290	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	5	8	theme	anti-HER-2	842:851	arg1	antibodies					853:862	anti-HER-2 antibodies	842:862	anti-HER-2 antibodies	842:862	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	3	9	theme	epithelial	440:449	arg1	cells					451:455	malignant epithelial cells	430:455	malignant epithelial cells	430:455	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	6	10	theme	adjacent	926:933	arg1	stroma					919:924	stroma	919:924	stroma adjacent to invasive areas in SC and CMT	919:965	VCAN was highly expressed in stroma adjacent to invasive areas in SC and CMT.					
34399281	0	11	theme	myoepithelial	82:94	arg1	proliferation					96:108	myoepithelial proliferation	82:108	myoepithelial proliferation	82:108	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.					
34399281	0	12	from	Expression	0:9	arg1	carcinomas					55:64	canine mammary carcinomas	40:64	canine mammary carcinomas with or without myoepithelial proliferation	40:108	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.					
34399281	7	13	theme	adjacent	1033:1040	arg1	stroma					1026:1031	stroma	1026:1031	stroma adjacent to in situ	1026:1051	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	2	14	from	carcinoma	288:296	arg1	CMT					315:317	CMT	315:317	CMT	315:317	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	14	from	carcinoma	288:296	arg1	tumors					307:312	mixed tumors	301:312	mixed tumors (CMT)	301:318	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	15	theme	mixed	266:270	arg1	carcinoma					288:296	carcinoma	288:296	carcinoma in mixed tumors (CMT) of the canine mammary gland	288:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	15	theme	mixed	266:270	arg1	tumors					272:277	malignant mixed tumors	256:277	malignant mixed tumors	256:277	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	3	16	from	proliferation	480:492	arg1	stages					525:530	early stages	519:530	early stages of cancer	519:540	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	5	17	from	anti-EGFR	828:836	arg1	cases					870:874	32 cases	867:874	32 cases of SC or CMT	867:887	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	2	18	theme	malignant	256:264	arg1	carcinoma					288:296	carcinoma	288:296	carcinoma in mixed tumors (CMT) of the canine mammary gland	288:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	18	theme	malignant	256:264	arg1	tumors					272:277	malignant mixed tumors	256:277	malignant mixed tumors	256:277	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	4	19	from	receptors	614:622	arg1	areas					648:652	in situ and invasive areas	627:652	in situ and invasive areas of simple carcinomas (SC) and CMT	627:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	7	20	from	expression	1004:1013	arg1	areas					1069:1073	invasive areas	1060:1073	invasive areas	1060:1073	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	7	20	from	expression	1004:1013	arg1	stroma					1026:1031	stroma	1026:1031	stroma adjacent to in situ	1026:1051	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	8	21	theme	invasive	1178:1185	arg1	areas					1187:1191	invasive areas	1178:1191	invasive areas	1178:1191	In CMT, EGFR and HER-2 expressions were higher in situ compared to invasive areas.					
34399281	4	22	dep	in	627:628	arg1	situ					630:633	situ	630:633	situ	630:633	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	10	23	theme	stromal	1532:1538	arg1	composition					1540:1550	stromal composition	1532:1550	stromal composition	1532:1550	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	1	24	theme	complex	152:158	arg1	role					160:163	a complex role	150:163	a complex role	150:163	The proteoglycan versican (VCAN) plays a complex role in cancer.					
34399281	9	25	theme	increased	1207:1215	arg1	expression					1231:1240	increased CD44 and EGFR expression	1207:1240	increased CD44 and EGFR expression	1207:1240	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	7	26	theme	invasive	1060:1067	arg1	areas					1069:1073	invasive areas	1060:1073	invasive areas	1060:1073	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	3	27	theme	surface	383:389	arg1	CD44					422:425	CD44	422:425	CD44	422:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	27	theme	surface	383:389	arg1	receptors					396:404	surface cell receptors EGFR, HER-2 and CD44	383:425	surface cell receptors EGFR, HER-2 and CD44	383:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	27	theme	surface	383:389	arg1	HER-2					412:416	HER-2	412:416	HER-2	412:416	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	27	theme	surface	383:389	arg1	EGFR					406:409	EGFR	406:409	EGFR	406:409	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	7	28	theme	corresponding	1083:1095	arg1	areas					1097:1101	corresponding areas	1083:1101	corresponding areas in SCs	1083:1108	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	7	29	from	areas	1097:1101	arg1	SCs					1106:1108	SCs	1106:1108	SCs	1106:1108	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	4	30	theme	proteoglycan	593:604	arg1	expression					574:583	the expression	570:583	the expression of this proteoglycan	570:604	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	4	30	theme	proteoglycan	593:604	arg1	receptors					614:622	its receptors	610:622	its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT	610:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	5	31	theme	SC	879:880	arg1	cases					870:874	32 cases	867:874	32 cases of SC or CMT	867:887	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	2	32	theme	canine	327:332	arg1	gland					342:346	the canine mammary gland	323:346	the canine mammary gland	323:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	10	33	theme	myoepithelial	1469:1481	arg1	proliferation					1483:1495	myoepithelial proliferation	1469:1495	myoepithelial proliferation	1469:1495	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	7	34	attach	presented	973:981	arg2	CMTs					968:971	CMTs	968:971	CMTs	968:971	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	7	34	attach	presented	973:981	arg1	areas					1097:1101	corresponding areas	1083:1101	corresponding areas in SCs	1083:1108	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	7	35	theme	VCAN	1018:1021	arg1	expression					1004:1013	comparatively higher expression	983:1013	comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas	983:1073	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	4	36	theme	carcinomas	664:673	arg1	areas					648:652	in situ and invasive areas	627:652	in situ and invasive areas of simple carcinomas (SC) and CMT	627:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	4	37	theme	CMT	684:686	arg1	areas					648:652	in situ and invasive areas	627:652	in situ and invasive areas of simple carcinomas (SC) and CMT	627:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	10	38	theme	versican	1321:1328	arg1	expression					1330:1339	versican expression	1321:1339	versican expression	1321:1339	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	7	39	dep	in	1045:1046	arg1	situ					1048:1051	situ	1048:1051	situ	1048:1051	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	9	40	theme	EGFR	1226:1229	arg1	expression					1231:1240	increased CD44 and EGFR expression	1207:1240	increased CD44 and EGFR expression	1207:1240	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	7	41	theme	higher	997:1002	arg1	expression					1004:1013	comparatively higher expression	983:1013	comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas	983:1073	CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs.					
34399281	5	42	theme	CMT	885:887	arg1	cases					870:874	32 cases	867:874	32 cases of SC or CMT	867:887	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	0	43	theme	VCAN	14:17	arg1	Expression					0:9	Expression	0:9	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.	0:109	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.					
34399281	3	44	theme	cellular	498:505	arg1	motility					507:514	cellular motility	498:514	cellular motility	498:514	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	4	45	from	expression	574:583	arg1	areas					648:652	in situ and invasive areas	627:652	in situ and invasive areas of simple carcinomas (SC) and CMT	627:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	10	46	from	increase	1569:1576	arg1	expression					1585:1594	the expression	1581:1594	the expression of versican	1581:1606	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	0	47	theme	receptors	27:35	arg1	Expression					0:9	Expression	0:9	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.	0:109	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.					
34399281	2	48	theme	mixed	301:305	arg1	CMT					315:317	CMT	315:317	CMT	315:317	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	48	theme	mixed	301:305	arg1	tumors					307:312	mixed tumors	301:312	mixed tumors (CMT)	301:318	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	4	49	theme	histological	746:757	arg1	types					759:763	these histological types	740:763	these histological types	740:763	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	2	50	from	invasion	228:235	arg1	carcinoma					288:296	carcinoma	288:296	carcinoma in mixed tumors (CMT) of the canine mammary gland	288:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	50	from	invasion	228:235	arg1	tumors					272:277	malignant mixed tumors	256:277	malignant mixed tumors	256:277	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	3	51	theme	cell	391:394	arg1	CD44					422:425	CD44	422:425	CD44	422:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	51	theme	cell	391:394	arg1	receptors					396:404	surface cell receptors EGFR, HER-2 and CD44	383:425	surface cell receptors EGFR, HER-2 and CD44	383:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	51	theme	cell	391:394	arg1	HER-2					412:416	HER-2	412:416	HER-2	412:416	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	51	theme	cell	391:394	arg1	EGFR					406:409	EGFR	406:409	EGFR	406:409	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	2	52	theme	gland	342:346	arg1	carcinoma					288:296	carcinoma	288:296	carcinoma in mixed tumors (CMT) of the canine mammary gland	288:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	10	53	theme	tumor	1505:1509	arg1	type					1511:1514	this tumor type	1500:1514	this tumor type	1500:1514	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	3	54	theme	cancer	535:540	arg1	stages					525:530	early stages	519:530	early stages of cancer	519:540	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	0	55	theme	canine	40:45	arg1	carcinomas					55:64	canine mammary carcinomas	40:64	canine mammary carcinomas with or without myoepithelial proliferation	40:108	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.					
34399281	10	56	theme	higher	1406:1411	arg1	levels					1413:1418	higher levels	1406:1418	higher levels	1406:1418	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	2	57	theme	molecule	199:206	arg1	expression					180:189	The expression	176:189	The expression of this molecule	176:206	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	57	theme	molecule	199:206	arg1	related					217:223	related	217:223	related	217:223	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	10	58	theme	versican	1599:1606	arg1	expression					1585:1594	the expression	1581:1594	the expression of versican	1581:1606	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	8	59	theme	EGFR	1119:1122	arg1	expressions					1134:1144	EGFR and HER-2 expressions	1119:1144	EGFR and HER-2 expressions	1119:1144	In CMT, EGFR and HER-2 expressions were higher in situ compared to invasive areas.					
34399281	9	60	theme	invasive	1255:1262	arg1	areas					1264:1268	invasive areas	1255:1268	invasive areas in SC compared to CMT	1255:1290	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	3	61	with	interaction	366:376	arg1	CD44					422:425	CD44	422:425	CD44	422:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	61	with	interaction	366:376	arg1	receptors					396:404	surface cell receptors EGFR, HER-2 and CD44	383:425	surface cell receptors EGFR, HER-2 and CD44	383:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	61	with	interaction	366:376	arg1	HER-2					412:416	HER-2	412:416	HER-2	412:416	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	61	with	interaction	366:376	arg1	EGFR					406:409	EGFR	406:409	EGFR	406:409	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	1	62	theme	proteoglycan	115:126	arg1	versican					128:135	The proteoglycan versican	111:135	The proteoglycan versican (VCAN)	111:142	The proteoglycan versican (VCAN) plays a complex role in cancer.					
34399281	1	62	theme	proteoglycan	115:126	arg1	VCAN					138:141	VCAN	138:141	VCAN	138:141	The proteoglycan versican (VCAN) plays a complex role in cancer.					
34399281	5	63	from	antibodies	853:862	arg1	cases					870:874	32 cases	867:874	32 cases of SC or CMT	867:887	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	4	64	theme	simple	657:662	arg1	SC					676:677	SC	676:677	SC	676:677	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	4	64	theme	simple	657:662	arg1	carcinomas					664:673	simple carcinomas	657:673	simple carcinomas (SC)	657:678	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	2	65	from	progression	241:251	arg1	carcinoma					288:296	carcinoma	288:296	carcinoma in mixed tumors (CMT) of the canine mammary gland	288:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	2	65	from	progression	241:251	arg1	tumors					272:277	malignant mixed tumors	256:277	malignant mixed tumors	256:277	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	9	66	located	found	1246:1250	arg1	areas					1264:1268	invasive areas	1255:1268	invasive areas in SC compared to CMT	1255:1290	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	9	66	located	found	1246:1250	arg1	contrast					1197:1204	contrast	1197:1204	contrast	1197:1204	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	9	66	located	found	1246:1250	arg2	expression					1231:1240	increased CD44 and EGFR expression	1207:1240	increased CD44 and EGFR expression	1207:1240	In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT.					
34399281	3	67	dep	receptors	396:404	arg1	CD44					422:425	CD44	422:425	CD44	422:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	67	dep	receptors	396:404	arg1	receptors					396:404	surface cell receptors EGFR, HER-2 and CD44	383:425	surface cell receptors EGFR, HER-2 and CD44	383:425	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	67	dep	receptors	396:404	arg1	HER-2					412:416	HER-2	412:416	HER-2	412:416	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	3	67	dep	receptors	396:404	arg1	EGFR					406:409	EGFR	406:409	EGFR	406:409	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	5	68	from	anti-CD44	817:825	arg1	cases					870:874	32 cases	867:874	32 cases of SC or CMT	867:887	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	3	69	from	motility	507:514	arg1	stages					525:530	early stages	519:530	early stages of cancer	519:540	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	10	70	from	invasiveness	1370:1381	arg1	SC					1386:1387	SC	1386:1387	SC	1386:1387	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	10	70	from	invasiveness	1370:1381	arg1	CMT					1393:1395	CMT	1393:1395	CMT	1393:1395	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	10	71	attach	presence	1457:1464	arg2	proliferation					1483:1495	myoepithelial proliferation	1469:1495	myoepithelial proliferation	1469:1495	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	10	71	attach	presence	1457:1464	arg1	type					1511:1514	this tumor type	1500:1514	this tumor type	1500:1514	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	3	72	from	interaction	366:376	arg1	cells					451:455	malignant epithelial cells	430:455	malignant epithelial cells	430:455	In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer.					
34399281	4	73	theme	in	627:628	arg1	areas					648:652	in situ and invasive areas	627:652	in situ and invasive areas of simple carcinomas (SC) and CMT	627:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
34399281	2	74	theme	mammary	334:340	arg1	gland					342:346	the canine mammary gland	323:346	the canine mammary gland	323:346	The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland.					
34399281	0	75	theme	mammary	47:53	arg1	carcinomas					55:64	canine mammary carcinomas	40:64	canine mammary carcinomas with or without myoepithelial proliferation	40:108	Expression of VCAN and its receptors in canine mammary carcinomas with or without myoepithelial proliferation.					
34399281	8	76	theme	HER-2	1128:1132	arg1	expressions					1134:1144	EGFR and HER-2 expressions	1119:1144	EGFR and HER-2 expressions	1119:1144	In CMT, EGFR and HER-2 expressions were higher in situ compared to invasive areas.					
34399281	6	77	theme	invasive	938:945	arg1	areas					947:951	invasive areas	938:951	invasive areas in SC and CMT	938:965	VCAN was highly expressed in stroma adjacent to invasive areas in SC and CMT.					
34399281	5	78	from	anti-VCAN	806:814	arg1	cases					870:874	32 cases	867:874	32 cases of SC or CMT	867:887	Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT.					
34399281	10	79	theme	proliferation	1483:1495	arg1	presence					1457:1464	the presence	1453:1464	the presence of myoepithelial proliferation in this tumor type	1453:1514	These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.					
34399281	4	80	theme	invasive	639:646	arg1	areas					648:652	in situ and invasive areas	627:652	in situ and invasive areas of simple carcinomas (SC) and CMT	627:686	We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types.					
33737185	7	0	theme	aspects	1298:1304	arg1	effectiveness					1270:1282	The effectiveness	1266:1282	The effectiveness of electrical aspects for CNF films	1266:1318	The effectiveness of electrical aspects for CNF films are well correlated with the crystallinity and thermal properties, associated with it's composition of different coconut's part.					
33737185	5	1	theme	films	1068:1072	arg1	properties					1050:1059	the modulus elasticity, piezoelectric and dielectric properties	997:1059	the modulus elasticity, piezoelectric and dielectric properties of the films	997:1072	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	4	2	link	husk-derived	796:807	arg1	CNF					809:811	coconut husk-derived CNF	788:811	coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width	788:876	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	0	3	theme	mechanical	83:92	arg1	properties					109:118	Physicochemical, mechanical and electrical properties	66:118	Physicochemical, mechanical and electrical properties	66:118	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	3	4	theme	thermal	733:739	arg1	properties					741:750	thermal properties	733:750	thermal properties	733:750	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	1	5	theme	cellulose	168:176	arg1	CNF					191:193	CNF	191:193	CNF	191:193	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	1	5	theme	cellulose	168:176	arg1	nanofibrils					178:188	cellulose nanofibrils	168:188	cellulose nanofibrils (CNF) film	168:199	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	4	6	theme	husk-derived	796:807	arg1	CNF					809:811	coconut husk-derived CNF	788:811	coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width	788:876	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	2	7	theme	CNF	556:558	arg1	preparation					560:570	CNF preparation	556:570	CNF preparation	556:570	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	3	8	theme	properties	741:750	arg1	terms					633:637	terms	633:637	terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties	633:750	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	3	9	theme	Physicochemical	573:587	arg1	properties					589:598	Physicochemical properties	573:598	Physicochemical properties of prepared CNFs	573:615	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	0	10	theme	electrical	98:107	arg1	properties					109:118	Physicochemical, mechanical and electrical properties	66:118	Physicochemical, mechanical and electrical properties	66:118	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	7	11	dep	crystallinity	1349:1361	arg1	the					1345:1347	the	1345:1347	the	1345:1347	The effectiveness of electrical aspects for CNF films are well correlated with the crystallinity and thermal properties, associated with it's composition of different coconut's part.					
33737185	5	12	theme	casting	971:977	arg1	method					979:984	solvent casting method	963:984	solvent casting method	963:984	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	6	13	theme	husk-derived	1196:1207	arg1	8.39 GPa					1229:1236	8.39 GPa	1229:1236	8.39 GPa	1229:1236	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	6	13	theme	husk-derived	1196:1207	arg1	film					1213:1216	the coconut husk-derived CNF film	1184:1216	the coconut husk-derived CNF film	1184:1216	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	2	14	theme	purified	362:369	arg1	cellulose					371:379	The chemically purified cellulose	347:379	The chemically purified cellulose	347:379	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	0	15	attach	derived	35:41	arg1	residues					56:63	coconut residues	48:63	coconut residues	48:63	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	0	15	attach	derived	35:41	arg2	films					29:33	TEMPO-oxidized nanocellulose films	0:33	TEMPO-oxidized nanocellulose films derived from coconut residues	0:63	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	0	16	dep	properties	109:118	arg1	films					29:33	TEMPO-oxidized nanocellulose films	0:33	TEMPO-oxidized nanocellulose films derived from coconut residues	0:63	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	7	17	theme	different	1423:1431	arg1	part					1443:1446	different coconut's part	1423:1446	different coconut's part	1423:1446	The effectiveness of electrical aspects for CNF films are well correlated with the crystallinity and thermal properties, associated with it's composition of different coconut's part.					
33737185	1	18	theme	nanofibrils	178:188	arg1	film					196:199	cellulose nanofibrils (CNF) film	168:199	cellulose nanofibrils (CNF) film	168:199	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	2	19	theme	-mediated	532:540	arg1	oxidation					542:550	2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation	480:550	2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation	480:550	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	5	20	theme	elasticity	1009:1018	arg1	properties					1050:1059	the modulus elasticity, piezoelectric and dielectric properties	997:1059	the modulus elasticity, piezoelectric and dielectric properties of the films	997:1072	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	6	21	theme	modulus	1171:1177	arg1	value					1154:1158	a higher value	1145:1158	a higher value of elastic modulus	1145:1177	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	0	22	theme	nanocellulose	15:27	arg1	films					29:33	TEMPO-oxidized nanocellulose films	0:33	TEMPO-oxidized nanocellulose films derived from coconut residues	0:63	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	6	23	theme	elastic	1163:1169	arg1	modulus					1171:1177	elastic modulus	1163:1177	elastic modulus	1163:1177	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	3	24	theme	CNFs	612:615	arg1	properties					589:598	Physicochemical properties	573:598	Physicochemical properties of prepared CNFs	573:615	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	6	25	theme	shell-derived	1115:1127	arg1	film					1133:1136	coconut shell-derived CNF film	1107:1136	coconut shell-derived CNF film	1107:1136	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	0	26	theme	TEMPO-oxidized	0:13	arg1	films					29:33	TEMPO-oxidized nanocellulose films	0:33	TEMPO-oxidized nanocellulose films derived from coconut residues	0:63	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	4	27	with	characteristics	844:858	arg1	width					872:876	fibres width	865:876	fibres width	865:876	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	4	28	theme	coconut	788:794	arg1	CNF					809:811	coconut husk-derived CNF	788:811	coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width	788:876	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	2	29	theme	different	399:407	arg1	parts					409:413	different parts	399:413	different parts of coconut (coconut shell and its husk)	399:453	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	1	30	theme	bio-based	279:287	arg1	membranes					300:308	bio-based conductive membranes	279:308	bio-based conductive membranes that assembled into supercapacitors	279:344	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	5	31	theme	piezoelectric	1021:1033	arg1	properties					1050:1059	the modulus elasticity, piezoelectric and dielectric properties	997:1059	the modulus elasticity, piezoelectric and dielectric properties of the films	997:1072	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	1	32	theme	film	196:199	arg1	development					153:163	the development	149:163	the development of cellulose nanofibrils (CNF) film	149:199	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	1	33	theme	conductive	289:298	arg1	membranes					300:308	bio-based conductive membranes	279:308	bio-based conductive membranes that assembled into supercapacitors	279:344	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	4	34	link	shell-derived	766:778	arg1	CNF					780:782	coconut shell-derived CNF	758:782	coconut shell-derived CNF	758:782	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	3	35	theme	functionalities	692:706	arg1	terms					633:637	terms	633:637	terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties	633:750	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	3	36	theme	prepared	603:610	arg1	CNFs					612:615	prepared CNFs	603:615	prepared CNFs	603:615	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	3	37	dep	characteristics	651:665	arg1	crystallinity					669:681	& crystallinity	667:681	& crystallinity	667:681	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	0	38	theme	coconut	48:54	arg1	residues					56:63	coconut residues	48:63	coconut residues	48:63	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	7	39	theme	thermal	1367:1373	arg1	properties					1375:1384	thermal properties	1367:1384	thermal properties	1367:1384	The effectiveness of electrical aspects for CNF films are well correlated with the crystallinity and thermal properties, associated with it's composition of different coconut's part.					
33737185	6	40	link	husk-derived	1196:1207	arg1	8.39 GPa					1229:1236	8.39 GPa	1229:1236	8.39 GPa	1229:1236	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	6	40	link	husk-derived	1196:1207	arg1	film					1213:1216	the coconut husk-derived CNF film	1184:1216	the coconut husk-derived CNF film	1184:1216	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	6	41	theme	higher	1147:1152	arg1	value					1154:1158	a higher value	1145:1158	a higher value of elastic modulus	1145:1177	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	2	42	theme	coconut	427:433	arg1	shell					435:439	coconut shell	427:439	coconut shell	427:439	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	2	43	theme	coconut	418:424	arg1	parts					409:413	different parts	399:413	different parts of coconut (coconut shell and its husk)	399:453	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	6	44	theme	Mechanical	1075:1084	arg1	study					1086:1090	Mechanical study	1075:1090	Mechanical study	1075:1090	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	5	45	theme	CNF	928:930	arg1	films					932:936	CNF films	928:936	CNF films	928:936	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	6	46	theme	CNF	1129:1131	arg1	film					1133:1136	coconut shell-derived CNF film	1107:1136	coconut shell-derived CNF film	1107:1136	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	3	47	theme	surface	684:690	arg1	functionalities					692:706	surface functionalities	684:706	surface functionalities	684:706	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	1	48	theme	present	125:131	arg1	work					133:136	The present work	121:136	The present work	121:136	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	7	49	theme	electrical	1287:1296	arg1	aspects					1298:1304	electrical aspects	1287:1304	electrical aspects for CNF films	1287:1318	The effectiveness of electrical aspects for CNF films are well correlated with the crystallinity and thermal properties, associated with it's composition of different coconut's part.					
33737185	4	50	theme	coconut	758:764	arg1	CNF					780:782	coconut shell-derived CNF	758:782	coconut shell-derived CNF	758:782	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	4	51	theme	shell-derived	766:778	arg1	CNF					780:782	coconut shell-derived CNF	758:782	coconut shell-derived CNF	758:782	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	4	52	theme	fibres	865:870	arg1	width					872:876	fibres width	865:876	fibres width	865:876	Both coconut shell-derived CNF and coconut husk-derived CNF fulfilled with nanocellulose's characteristics with fibres width ranged of 70-120 nm and 150-330 nm, respectively.					
33737185	3	53	theme	&	667:667	arg1	crystallinity					669:681	& crystallinity	667:681	& crystallinity	667:681	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	6	54	theme	coconut	1107:1113	arg1	film					1133:1136	coconut shell-derived CNF film	1107:1136	coconut shell-derived CNF film	1107:1136	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	6	55	theme	CNF	1209:1211	arg1	8.39 GPa					1229:1236	8.39 GPa	1229:1236	8.39 GPa	1229:1236	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	6	55	theme	CNF	1209:1211	arg1	film					1213:1216	the coconut husk-derived CNF film	1184:1216	the coconut husk-derived CNF film	1184:1216	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	3	56	theme	chemical	642:649	arg1	characteristics					651:665	chemical characteristics	642:665	chemical characteristics	642:665	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	1	57	theme	sustainable	219:229	arg1	resources					239:247	sustainable biomass resources	219:247	sustainable biomass resources	219:247	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	3	58	theme	surface	709:715	arg1	morphology					717:726	surface morphology	709:726	surface morphology	709:726	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	5	59	theme	dielectric	1039:1048	arg1	properties					1050:1059	the modulus elasticity, piezoelectric and dielectric properties	997:1059	the modulus elasticity, piezoelectric and dielectric properties of the films	997:1072	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	5	60	theme	modulus	1001:1007	arg1	properties					1050:1059	the modulus elasticity, piezoelectric and dielectric properties	997:1059	the modulus elasticity, piezoelectric and dielectric properties of the films	997:1072	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	1	61	theme	biomass	231:237	arg1	resources					239:247	sustainable biomass resources	219:247	sustainable biomass resources	219:247	The present work focuses on the development of cellulose nanofibrils (CNF) film that derived from sustainable biomass resources, which potentially to work as bio-based conductive membranes that assembled into supercapacitors.					
33737185	7	62	theme	CNF	1310:1312	arg1	films					1314:1318	CNF films	1310:1318	CNF films	1310:1318	The effectiveness of electrical aspects for CNF films are well correlated with the crystallinity and thermal properties, associated with it's composition of different coconut's part.					
33737185	3	63	theme	morphology	717:726	arg1	terms					633:637	terms	633:637	terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties	633:750	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	2	64	dep	coconut	418:424	arg1	shell					435:439	coconut shell	427:439	coconut shell	427:439	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	2	64	dep	coconut	418:424	arg1	husk					449:452	its husk	445:452	its husk	445:452	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	6	65	theme	coconut	1188:1194	arg1	8.39 GPa					1229:1236	8.39 GPa	1229:1236	8.39 GPa	1229:1236	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	6	65	theme	coconut	1188:1194	arg1	film					1213:1216	the coconut husk-derived CNF film	1184:1216	the coconut husk-derived CNF film	1184:1216	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
33737185	0	66	theme	Physicochemical	66:80	arg1	properties					109:118	Physicochemical, mechanical and electrical properties	66:118	Physicochemical, mechanical and electrical properties	66:118	TEMPO-oxidized nanocellulose films derived from coconut residues: Physicochemical, mechanical and electrical properties.					
33737185	2	67	attach	isolated	385:392	arg2	cellulose					371:379	The chemically purified cellulose	347:379	The chemically purified cellulose	347:379	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	2	67	attach	isolated	385:392	arg1	parts					409:413	different parts	399:413	different parts of coconut (coconut shell and its husk)	399:453	The chemically purified cellulose was isolated from different parts of coconut (coconut shell and its husk) and further subjected to 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation for CNF preparation.					
33737185	3	68	theme	characteristics	651:665	arg1	terms					633:637	terms	633:637	terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties	633:750	Physicochemical properties of prepared CNFs were studied in terms of chemical characteristics & crystallinity, surface functionalities, surface morphology, and thermal properties.					
33737185	5	69	theme	solvent	963:969	arg1	method					979:984	solvent casting method	963:984	solvent casting method	963:984	CNF films were further prepared by solvent casting method to measure the modulus elasticity, piezoelectric and dielectric properties of the films.					
33737185	6	70	link	shell-derived	1115:1127	arg1	film					1133:1136	coconut shell-derived CNF film	1107:1136	coconut shell-derived CNF film	1107:1136	Mechanical study indicated that coconut shell-derived CNF film showed a higher value of elastic modulus than the coconut husk-derived CNF film, which was 8.39 GPa and 5.36 GPa, respectively.					
34146688	0	0	theme	Homarus	82:88	arg1	performance					126:136	performance	126:136	performance	126:136	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	0	theme	Homarus	82:88	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	0	theme	Homarus	82:88	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	7	1	theme	feed	1214:1217	arg1	intake					1219:1224	feed intake	1214:1224	feed intake	1214:1224	Regardless the dietary treatment, feed intake, cephalothorax protein and glucose, and abdominal glycogen and glucose levels decreased at colder temperature.					
34146688	1	2	theme	rearing	147:153	arg1	temperatures					155:166	Optimal rearing temperatures	139:166	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture	139:219	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	4	3	theme	temperature	854:864	arg1	group					866:870	each temperature group	849:870	each temperature group	849:870	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	8	4	theme	HC-fed	1503:1508	arg1	lobsters					1510:1517	HC-fed lobsters	1503:1517	HC-fed lobsters	1503:1517	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	2	5	theme	energy	555:560	arg1	metabolism					562:571	energy metabolism	555:571	energy metabolism	555:571	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	5	6	theme	temperature	1040:1050	arg1	group					1052:1056	each temperature group	1035:1056	each temperature group	1035:1056	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	8	7	from	reduction	1435:1443	arg1	glycogen					1462:1469	cephalothorax glycogen	1448:1469	cephalothorax glycogen	1448:1469	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	9	8	theme	feed	1702:1705	arg1	efficiency					1707:1716	feed efficiency	1702:1716	feed efficiency	1702:1716	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	5	9	used	used	1005:1008	arg2	diet					1023:1026	a control diet	1013:1026	a control diet within each temperature group	1013:1056	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	5	9	used	used	1005:1008	arg2	AK					997:998	AK	997:998	AK	997:998	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	5	9	used	used	1005:1008	arg2	krill					990:994	Antarctic krill	980:994	Antarctic krill (AK)	980:999	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	1	10	theme	aquatic	258:264	arg1	ecosystems					266:275	their aquatic ecosystems	252:275	their aquatic ecosystems	252:275	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	2	11	theme	juvenile	535:542	arg1	responses					573:581	juvenile growth and energy metabolism responses	535:581	juvenile growth and energy metabolism responses to temperature variation	535:606	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	0	12	from	Interactions	0:11	arg1	performance					126:136	performance	126:136	performance	126:136	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	12	from	Interactions	0:11	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	12	from	Interactions	0:11	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	6	13	theme	protein	1112:1118	arg1	concentrations					1120:1133	protein concentrations	1112:1133	protein concentrations	1112:1133	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	14	from	lactate	1099:1105	arg1	group					1158:1162	each group	1153:1162	each group	1153:1162	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	4	15	theme	experimental	897:908	arg1	diets					910:914	two experimental diets	893:914	two experimental diets	893:914	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	6	16	from	glycogen	1080:1087	arg1	group					1158:1162	each group	1153:1162	each group	1153:1162	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	8	17	from	lobsters	1510:1517	arg1	severe					1493:1498	severe	1493:1498	severe	1493:1498	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	8	17	from	lobsters	1510:1517	arg1	effect					1341:1346	The effect	1337:1346	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein)	1337:1482	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	8	18	from	protein	1475:1481	arg1	glycogen					1462:1469	cephalothorax glycogen	1448:1469	cephalothorax glycogen	1448:1469	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	6	19	from	glucose	1090:1096	arg1	group					1158:1162	each group	1153:1162	each group	1153:1162	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	20	from	growth	1072:1077	arg1	group					1158:1162	each group	1153:1162	each group	1153:1162	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	2	21	theme	dietary	404:410	arg1	composition					424:434	the dietary nutritional composition	400:434	the dietary nutritional composition	400:434	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	2	21	theme	dietary	404:410	arg1	important					439:447	important	439:447	important	439:447	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	0	22	theme	L.	100:101	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	22	theme	L.	100:101	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	1	23	theme	re-stocking	375:385	arg1	programs					387:394	re-stocking programs	375:394	re-stocking programs	375:394	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	8	24	dep	SGR	1380:1382	arg1	rate					1397:1400	rate declines	1397:1409	rate declines	1397:1409	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	7	25	theme	colder	1317:1322	arg1	temperature					1324:1334	colder temperature	1317:1334	colder temperature	1317:1334	Regardless the dietary treatment, feed intake, cephalothorax protein and glucose, and abdominal glycogen and glucose levels decreased at colder temperature.					
34146688	1	26	theme	European	172:179	arg1	gammarus					197:204	European lobster Homarus gammarus	172:204	European lobster Homarus gammarus in aquaculture	172:219	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	1	27	theme	programs	387:394	arg1	success					364:370	the success	360:370	the success of re-stocking programs	360:394	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	6	28	theme	gammarus	1141:1148	arg1	lactate					1099:1105	lactate	1099:1105	lactate	1099:1105	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	28	theme	gammarus	1141:1148	arg1	growth					1072:1077	growth	1072:1077	growth	1072:1077	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	28	theme	gammarus	1141:1148	arg1	glycogen					1080:1087	glycogen	1080:1087	glycogen	1080:1087	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	28	theme	gammarus	1141:1148	arg1	concentrations					1120:1133	protein concentrations	1112:1133	protein concentrations	1112:1133	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	28	theme	gammarus	1141:1148	arg1	intake					1064:1069	Feed intake	1059:1069	Feed intake	1059:1069	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	6	28	theme	gammarus	1141:1148	arg1	glucose					1090:1096	glucose	1090:1096	glucose	1090:1096	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	2	29	theme	temperature	586:596	arg1	variation					598:606	temperature variation	586:606	temperature variation	586:606	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	1	30	theme	lobster	181:187	arg1	gammarus					197:204	European lobster Homarus gammarus	172:204	European lobster Homarus gammarus in aquaculture	172:219	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	9	31	theme	H.	1584:1585	arg1	gammarus					1587:1594	juvenile H. gammarus	1575:1594	juvenile H. gammarus	1575:1594	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	1	32	theme	acclimating	281:291	arg1	juveniles					293:301	acclimating juveniles	281:301	acclimating juveniles to the prevailing temperatures before release	281:347	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	6	33	theme	H.	1138:1139	arg1	gammarus					1141:1148	H. gammarus	1138:1148	H. gammarus	1138:1148	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	8	34	from	severe	1493:1498	arg1	lobsters					1510:1517	HC-fed lobsters	1503:1517	HC-fed lobsters	1503:1517	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	1	35	theme	Homarus	189:195	arg1	gammarus					197:204	European lobster Homarus gammarus	172:204	European lobster Homarus gammarus in aquaculture	172:219	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	2	36	theme	optimal	453:459	arg1	performance					461:471	optimal performance	453:471	optimal performance of H. gammarus	453:486	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	8	37	dep	growth	1372:1377	arg1	moulting					1388:1395	moulting	1388:1395	moulting	1388:1395	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	8	37	dep	growth	1372:1377	arg1	SGR					1380:1382	SGR	1380:1382	SGR	1380:1382	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	1	38	from	ecosystems	266:275	arg1	prevalent					239:247	prevalent	239:247	prevalent	239:247	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	9	39	theme	lower	1554:1558	arg1	temperature					1560:1570	lower temperature	1554:1570	lower temperature	1554:1570	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	6	40	theme	Feed	1059:1062	arg1	intake					1064:1069	Feed intake	1059:1069	Feed intake	1059:1069	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	7	41	theme	dietary	1195:1201	arg1	treatment					1203:1211	Regardless the dietary treatment	1180:1211	Regardless the dietary treatment	1180:1211	Regardless the dietary treatment, feed intake, cephalothorax protein and glucose, and abdominal glycogen and glucose levels decreased at colder temperature.					
34146688	0	42	theme	temperature	16:26	arg1	Interactions					0:11	Interactions	0:11	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.	0:137	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	9	43	from	impact	1544:1549	arg1	gammarus					1587:1594	juvenile H. gammarus	1575:1594	juvenile H. gammarus	1575:1594	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	0	44	theme	energy	104:109	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	44	theme	energy	104:109	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	3	45	theme	trial	653:657	arg1	start					659:663	the trial start	649:663	the trial start	649:663	Prior to the trial start, the juveniles were divided into two groups.					
34146688	7	46	theme	glucose	1289:1295	arg1	levels					1297:1302	abdominal glycogen and glucose levels	1266:1302	levels	1297:1302	Regardless the dietary treatment, feed intake, cephalothorax protein and glucose, and abdominal glycogen and glucose levels decreased at colder temperature.					
34146688	2	47	theme	gammarus	479:486	arg1	performance					461:471	optimal performance	453:471	optimal performance of H. gammarus	453:486	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	4	48	dep	assigned	877:884	arg1	protein-rich					947:958	protein-rich	947:958	protein-rich	947:958	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	4	48	dep	assigned	877:884	arg1	HC					938:939	HC	938:939	HC	938:939	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	4	48	dep	assigned	877:884	arg1	HP					961:962	HP	961:962	HP	961:962	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	4	48	dep	assigned	877:884	arg1	carbohydrate-rich					919:935	carbohydrate-rich	919:935	carbohydrate-rich	919:935	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	0	49	theme	composition	40:50	arg1	Interactions					0:11	Interactions	0:11	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.	0:137	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	8	50	theme	energy	1416:1421	arg1	metabolism					1423:1432	energy metabolism	1416:1432	energy metabolism (reduction on cephalothorax glycogen and protein)	1416:1482	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	9	51	theme	temperature	1560:1570	arg1	impact					1544:1549	the impact	1540:1549	the impact of lower temperature on juvenile H. gammarus	1540:1594	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	7	52	theme	abdominal	1266:1274	arg1	glycogen					1276:1283	abdominal glycogen and glucose levels	1266:1302	glycogen	1276:1283	Regardless the dietary treatment, feed intake, cephalothorax protein and glucose, and abdominal glycogen and glucose levels decreased at colder temperature.					
34146688	6	53	from	intake	1064:1069	arg1	group					1158:1162	each group	1153:1162	each group	1153:1162	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	0	54	theme	dietary	32:38	arg1	composition					40:50	dietary composition	32:50	dietary composition	32:50	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	1	55	theme	prevailing	310:319	arg1	temperatures					321:332	the prevailing temperatures	306:332	the prevailing temperatures	306:332	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	2	56	theme	growth	544:549	arg1	responses					573:581	juvenile growth and energy metabolism responses	535:581	juvenile growth and energy metabolism responses to temperature variation	535:606	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	8	57	theme	lower	1351:1355	arg1	temperature					1357:1367	lower temperature	1351:1367	lower temperature	1351:1367	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	9	58	theme	optimized	1662:1670	arg1	diets					1672:1676	optimized diets	1662:1676	optimized diets not only for growth and feed efficiency but also for resilience to environmental variation	1662:1767	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	2	59	theme	nutritional	412:422	arg1	composition					424:434	the dietary nutritional composition	400:434	the dietary nutritional composition	400:434	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	2	59	theme	nutritional	412:422	arg1	important					439:447	important	439:447	important	439:447	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	0	60	theme	juvenile	55:62	arg1	performance					126:136	performance	126:136	performance	126:136	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	60	theme	juvenile	55:62	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	60	theme	juvenile	55:62	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	4	61	theme	diets	910:914	arg1	one					886:888	one	886:888	one	886:888	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	4	61	theme	diets	910:914	arg1	diets					910:914	two experimental diets	893:914	two experimental diets	893:914	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	1	62	from	prevalent	239:247	arg1	ecosystems					266:275	their aquatic ecosystems	252:275	their aquatic ecosystems	252:275	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	4	63	theme	24-day	806:811	arg1	period					813:818	a 24-day period	804:818	a 24-day period	804:818	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	5	64	theme	control	1015:1021	arg1	krill					990:994	Antarctic krill	980:994	Antarctic krill (AK)	980:999	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	5	64	theme	control	1015:1021	arg1	diet					1023:1026	a control diet	1013:1026	a control diet within each temperature group	1013:1056	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	2	65	theme	H.	476:477	arg1	gammarus					479:486	H. gammarus	476:486	H. gammarus	476:486	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	2	66	theme	metabolism	562:571	arg1	responses					573:581	juvenile growth and energy metabolism responses	535:581	juvenile growth and energy metabolism responses to temperature variation	535:606	As the dietary nutritional composition is important for optimal performance of H. gammarus, in this study we aimed to investigate whether juvenile growth and energy metabolism responses to temperature variation could be modulated by the diet.					
34146688	8	67	theme	temperature	1357:1367	arg1	severe					1493:1498	severe	1493:1498	severe	1493:1498	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	8	67	theme	temperature	1357:1367	arg1	effect					1341:1346	The effect	1337:1346	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein)	1337:1482	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	1	68	from	gammarus	197:204	arg1	aquaculture					209:219	aquaculture	209:219	aquaculture	209:219	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	8	69	dep	rate	1397:1400	arg1	declines					1402:1409	declines	1402:1409	declines	1402:1409	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	5	70	theme	Antarctic	980:988	arg1	krill					990:994	Antarctic krill	980:994	Antarctic krill (AK)	980:999	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	5	70	theme	Antarctic	980:988	arg1	diet					1023:1026	a control diet	1013:1026	a control diet within each temperature group	1013:1056	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	5	70	theme	Antarctic	980:988	arg1	AK					997:998	AK	997:998	AK	997:998	Antarctic krill (AK) was used as a control diet within each temperature group.					
34146688	8	71	from	effect	1341:1346	arg1	metabolism					1423:1432	energy metabolism	1416:1432	energy metabolism (reduction on cephalothorax glycogen and protein)	1416:1482	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	8	71	from	effect	1341:1346	arg1	growth					1372:1377	growth	1372:1377	growth (SGR and moulting rate declines)	1372:1410	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	4	72	dep	carbohydrate-rich	919:935	arg1	feed					974:977	extruded feed	965:977	extruded feed	965:977	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	0	73	theme	lobster	73:79	arg1	performance					126:136	performance	126:136	performance	126:136	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	73	theme	lobster	73:79	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	73	theme	lobster	73:79	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	8	74	dep	metabolism	1423:1432	arg1	reduction					1435:1443	reduction	1435:1443	reduction on cephalothorax glycogen	1435:1469	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	8	74	dep	metabolism	1423:1432	arg1	protein					1475:1481	protein	1475:1481	protein	1475:1481	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34146688	7	75	theme	cephalothorax	1227:1239	arg1	protein					1241:1247	cephalothorax protein	1227:1247	cephalothorax protein	1227:1247	Regardless the dietary treatment, feed intake, cephalothorax protein and glucose, and abdominal glycogen and glucose levels decreased at colder temperature.					
34146688	9	76	theme	environmental	1745:1757	arg1	variation					1759:1767	environmental variation	1745:1767	environmental variation	1745:1767	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	6	77	from	concentrations	1120:1133	arg1	group					1158:1162	each group	1153:1162	each group	1153:1162	Feed intake, growth, glycogen, glucose, lactate, and protein concentrations of H. gammarus in each group were evaluated.					
34146688	0	78	theme	European	64:71	arg1	performance					126:136	performance	126:136	performance	126:136	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	78	theme	European	64:71	arg1	metabolism					111:120	L.) energy metabolism	100:120	L.) energy metabolism	100:120	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	0	78	theme	European	64:71	arg1	gammarus					90:97	juvenile European lobster (Homarus gammarus	55:97	juvenile European lobster (Homarus gammarus	55:97	Interactions of temperature and dietary composition on juvenile European lobster (Homarus gammarus, L.) energy metabolism and performance.					
34146688	4	79	theme	extruded	965:972	arg1	feed					974:977	extruded feed	965:977	extruded feed	965:977	One was maintained at 19 °C and the other gradually adapted to 13 °C. From this point and for a 24-day period, juveniles (~ 100 mg) within each temperature group were assigned one of two experimental diets: a carbohydrate-rich (HC) or a protein-rich (HP) extruded feed.					
34146688	1	80	theme	Optimal	139:145	arg1	temperatures					155:166	Optimal rearing temperatures	139:166	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture	139:219	Optimal rearing temperatures for European lobster Homarus gammarus in aquaculture differ from those prevalent in their aquatic ecosystems and acclimating juveniles to the prevailing temperatures before release may aid in the success of re-stocking programs.					
34146688	9	81	theme	juvenile	1575:1582	arg1	gammarus					1587:1594	juvenile H. gammarus	1575:1594	juvenile H. gammarus	1575:1594	Results showed that the impact of lower temperature on juvenile H. gammarus can be modulated by diet highlighting the importance of designing optimized diets not only for growth and feed efficiency but also for resilience to environmental variation.					
34146688	8	82	theme	cephalothorax	1448:1460	arg1	glycogen					1462:1469	cephalothorax glycogen	1448:1469	cephalothorax glycogen	1448:1469	The effect of lower temperature on growth (SGR and moulting rate declines) and energy metabolism (reduction on cephalothorax glycogen and protein) was more severe in HC-fed lobsters.					
34763016	3	0	from	damage	385:390	arg1	tissue					436:441	colon tissue	430:441	colon tissue	430:441	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	8	1	dep	induced	1211:1217	arg1	could					1205:1209	could	1205:1209	could	1205:1209	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	8	2	theme	microbiota	1130:1139	arg1	experiment					1157:1166	the gut microbiota transplantation experiment	1122:1166	the gut microbiota transplantation experiment	1122:1166	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	2	3	from	colitis	268:274	arg1	mice					279:282	mice	279:282	mice	279:282	In this study, we examined the effects of exposure to tebuconazole on colitis in mice and explored its underlying mechanism.					
34763016	0	4	theme	gut	72:74	arg1	microbiota					76:85	gut microbiota	72:85	gut microbiota	72:85	A common fungicide tebuconazole promotes colitis in mice via regulating gut microbiota.					
34763016	8	5	from	inflammation	1227:1238	arg1	mice					1243:1246	mice	1243:1246	mice	1243:1246	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	1	6	theme	natural	146:152	arg1	environment					154:164	the natural environment	142:164	the natural environment	142:164	As a common fungicide, tebuconazole are ubiquitous in the natural environment and poses many potential risks.					
34763016	10	7	theme	inflammatory	1490:1501	arg1	disease					1509:1515	inflammatory bowel disease	1490:1515	inflammatory bowel disease caused by pesticide intake	1490:1542	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	7	8	theme	significant	974:984	arg1	changes					986:992	significant changes	974:992	significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism	974:1090	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	3	9	theme	function-related	533:548	arg1	genes					550:554	barrier function-related genes	525:554	barrier function-related genes	525:554	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	10	10	theme	key	1475:1477	arg1	microbiota					1459:1468	the gut microbiota	1451:1468	the gut microbiota	1451:1468	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	10	10	theme	key	1475:1477	arg1	trigger					1479:1485	a key trigger	1473:1485	a key trigger of inflammatory bowel disease caused by pesticide intake	1473:1542	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	1	11	from	ubiquitous	128:137	arg1	environment					154:164	the natural environment	142:164	the natural environment	142:164	As a common fungicide, tebuconazole are ubiquitous in the natural environment and poses many potential risks.					
34763016	3	12	theme	genes	550:554	arg1	expression					511:520	the expression	507:520	the expression of barrier function-related genes	507:554	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	4	13	from	colitis	672:678	arg1	mice					683:686	mice	683:686	mice	683:686	Similarly, exposure to tebuconazole could also exacerbate DSS-induced colitis in mice.					
34763016	4	14	theme	DSS-induced	660:670	arg1	colitis					672:678	DSS-induced colitis	660:678	DSS-induced colitis in mice	660:686	Similarly, exposure to tebuconazole could also exacerbate DSS-induced colitis in mice.					
34763016	0	15	from	colitis	41:47	arg1	mice					52:55	mice	52:55	mice	52:55	A common fungicide tebuconazole promotes colitis in mice via regulating gut microbiota.					
34763016	10	16	theme	pesticide	1527:1535	arg1	intake					1537:1542	pesticide intake	1527:1542	pesticide intake	1527:1542	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	3	17	theme	barrier	525:531	arg1	genes					550:554	barrier function-related genes	525:554	barrier function-related genes	525:554	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	3	18	theme	colon	430:434	arg1	tissue					436:441	colon tissue	430:441	colon tissue	430:441	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	0	19	theme	common	2:7	arg1	tebuconazole					19:30	A common fungicide tebuconazole	0:30	A common fungicide tebuconazole	0:30	A common fungicide tebuconazole promotes colitis in mice via regulating gut microbiota.					
34763016	10	20	theme	disease	1509:1515	arg1	microbiota					1459:1468	the gut microbiota	1451:1468	the gut microbiota	1451:1468	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	10	20	theme	disease	1509:1515	arg1	trigger					1479:1485	a key trigger	1473:1485	a key trigger of inflammatory bowel disease caused by pesticide intake	1473:1542	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	7	21	theme	serum	956:960	arg1	disorders					939:947	metabolic profiles disorders	920:947	metabolic profiles disorders of the serum	920:960	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	3	22	theme	cell	409:412	arg1	infiltration					414:425	inflammatory cell infiltration	396:425	inflammatory cell infiltration	396:425	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	8	23	theme	colonic	1219:1225	arg1	inflammation					1227:1238	colonic inflammation	1219:1238	colonic inflammation in mice	1219:1246	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	10	24	theme	bowel	1503:1507	arg1	disease					1509:1515	inflammatory bowel disease	1490:1515	inflammatory bowel disease caused by pesticide intake	1490:1542	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	1	25	theme	many	176:179	arg1	risks					191:195	many potential risks	176:195	many potential risks	176:195	As a common fungicide, tebuconazole are ubiquitous in the natural environment and poses many potential risks.					
34763016	9	26	from	colitis	1357:1363	arg1	mice					1368:1371	mice	1368:1371	mice	1368:1371	Taken together, these results indicated that tebuconazole could induce colitis in mice via regulating gut microbiota.					
34763016	9	27	theme	gut	1388:1390	arg1	microbiota					1392:1401	gut microbiota	1388:1401	gut microbiota	1388:1401	Taken together, these results indicated that tebuconazole could induce colitis in mice via regulating gut microbiota.					
34763016	10	28	theme	gut	1455:1457	arg1	microbiota					1459:1468	the gut microbiota	1451:1468	the gut microbiota	1451:1468	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	10	28	theme	gut	1455:1457	arg1	trigger					1479:1485	a key trigger	1473:1485	a key trigger of inflammatory bowel disease caused by pesticide intake	1473:1542	Our findings strongly support the concept that the gut microbiota is a key trigger of inflammatory bowel disease caused by pesticide intake.					
34763016	3	29	from	inflammation	580:591	arg1	mice					596:599	mice	596:599	mice	596:599	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	1	30	theme	potential	181:189	arg1	risks					191:195	many potential risks	176:195	many potential risks	176:195	As a common fungicide, tebuconazole are ubiquitous in the natural environment and poses many potential risks.					
34763016	0	31	theme	fungicide	9:17	arg1	tebuconazole					19:30	A common fungicide tebuconazole	0:30	A common fungicide tebuconazole	0:30	A common fungicide tebuconazole promotes colitis in mice via regulating gut microbiota.					
34763016	6	32	theme	mice	879:882	arg1	Akkermansia					864:874	Akkermansia	864:874	Akkermansia of mice	864:882	In particular, tebuconazole significantly increases the relative abundance of Akkermansia of mice.					
34763016	1	33	theme	common	93:98	arg1	fungicide					100:108	a common fungicide	91:108	a common fungicide	91:108	As a common fungicide, tebuconazole are ubiquitous in the natural environment and poses many potential risks.					
34763016	5	34	theme	gut	770:772	arg1	microbiota					774:783	the gut microbiota	766:783	the gut microbiota	766:783	In addition, we found that tebuconazole also could change the composition of the gut microbiota.					
34763016	2	35	theme	underlying	301:310	arg1	mechanism					312:320	its underlying mechanism	297:320	its underlying mechanism	297:320	In this study, we examined the effects of exposure to tebuconazole on colitis in mice and explored its underlying mechanism.					
34763016	7	36	theme	acid	1076:1079	arg1	metabolism					1081:1090	amino acid metabolism	1070:1090	amino acid metabolism	1070:1090	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	2	37	from	effects	229:235	arg1	colitis					268:274	colitis	268:274	colitis in mice	268:282	In this study, we examined the effects of exposure to tebuconazole on colitis in mice and explored its underlying mechanism.					
34763016	3	38	theme	inflammatory	396:407	arg1	infiltration					414:425	inflammatory cell infiltration	396:425	inflammatory cell infiltration	396:425	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	5	39	theme	microbiota	774:783	arg1	composition					751:761	the composition	747:761	the composition of the gut microbiota	747:783	In addition, we found that tebuconazole also could change the composition of the gut microbiota.					
34763016	6	40	theme	Akkermansia	864:874	arg1	abundance					851:859	the relative abundance	838:859	the relative abundance of Akkermansia of mice	838:882	In particular, tebuconazole significantly increases the relative abundance of Akkermansia of mice.					
34763016	7	41	theme	metabolites	1022:1032	arg1	contents					1010:1017	the relative contents	997:1017	the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism	997:1090	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	3	42	theme	structural	374:383	arg1	damage					385:390	structural damage	374:390	structural damage	374:390	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	8	43	theme	gut	1126:1128	arg1	experiment					1157:1166	the gut microbiota transplantation experiment	1122:1166	the gut microbiota transplantation experiment	1122:1166	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	3	44	theme	colonic	572:578	arg1	inflammation					580:591	the colonic inflammation	568:591	the colonic inflammation in mice	568:599	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	8	45	theme	transplantation	1141:1155	arg1	experiment					1157:1166	the gut microbiota transplantation experiment	1122:1166	the gut microbiota transplantation experiment	1122:1166	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	3	46	theme	inflammation-related	471:490	arg1	genes					492:496	inflammation-related genes	471:496	inflammation-related genes	471:496	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	8	47	theme	gut	1253:1255	arg1	manner					1278:1283	a gut microbiota-dependent manner	1251:1283	a gut microbiota-dependent manner	1251:1283	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	7	48	theme	amino	1070:1074	arg1	metabolism					1081:1090	amino acid metabolism	1070:1090	amino acid metabolism	1070:1090	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	3	49	theme	genes	492:496	arg1	expression					457:466	the expression	453:466	the expression of inflammation-related genes	453:496	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	7	50	theme	metabolic	920:928	arg1	disorders					939:947	metabolic profiles disorders	920:947	metabolic profiles disorders of the serum	920:960	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	7	51	theme	relative	1001:1008	arg1	contents					1010:1017	the relative contents	997:1017	the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism	997:1090	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	7	52	theme	glycolipid	1044:1053	arg1	metabolism					1055:1064	glycolipid metabolism	1044:1064	glycolipid metabolism	1044:1064	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	6	53	theme	relative	842:849	arg1	abundance					851:859	the relative abundance	838:859	the relative abundance of Akkermansia of mice	838:882	In particular, tebuconazole significantly increases the relative abundance of Akkermansia of mice.					
34763016	7	54	theme	profiles	930:937	arg1	disorders					939:947	metabolic profiles disorders	920:947	metabolic profiles disorders of the serum	920:960	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	2	55	theme	exposure	240:247	arg1	effects					229:235	the effects	225:235	the effects of exposure to tebuconazole on colitis in mice	225:282	In this study, we examined the effects of exposure to tebuconazole on colitis in mice and explored its underlying mechanism.					
34763016	8	56	theme	microbiota-dependent	1257:1276	arg1	manner					1278:1283	a gut microbiota-dependent manner	1251:1283	a gut microbiota-dependent manner	1251:1283	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	3	57	from	infiltration	414:425	arg1	tissue					436:441	colon tissue	430:441	colon tissue	430:441	Specifically, exposure to tebuconazole could cause structural damage and inflammatory cell infiltration in colon tissue, activate the expression of inflammation-related genes, disrupt the expression of barrier function-related genes, and induce the colonic inflammation in mice.					
34763016	7	58	from	changes	986:992	arg1	contents					1010:1017	the relative contents	997:1017	the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism	997:1090	Moreover, tebuconazole resulted in metabolic profiles disorders of the serum, leading to significant changes in the relative contents of metabolites involving glycolipid metabolism and amino acid metabolism.					
34763016	8	59	theme	experiment	1157:1166	arg1	results					1111:1117	the results	1107:1117	the results of the gut microbiota transplantation experiment	1107:1166	Particularly, the results of the gut microbiota transplantation experiment showed that exposure to tebuconazole could induced colonic inflammation in mice in a gut microbiota-dependent manner.					
34763016	1	60	from	environment	154:164	arg1	ubiquitous					128:137	ubiquitous	128:137	ubiquitous	128:137	As a common fungicide, tebuconazole are ubiquitous in the natural environment and poses many potential risks.					
33586859	5	0	theme	ligand	755:760	arg1	binding					762:768	ligand binding	755:768	ligand binding	755:768	CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS.					
33586859	6	1	theme	serine	974:979	arg1	residue					981:987	the serine residue	970:987	the serine residue	970:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	6	2	theme	secretory	887:895	arg1	pathway					897:903	the secretory pathway	883:903	the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue	883:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	1	3	theme	molecular	156:164	arg1	composition					166:176	the molecular composition	152:176	the molecular composition of synapses	152:188	Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses.					
33586859	2	4	theme	distinct	308:315	arg1	ligands					317:323	distinct ligands	308:323	distinct ligands	308:323	Diversification of neurexins in numerous isoforms is believed to confer synapse-specific properties by engaging with distinct ligands.					
33586859	4	5	theme	secretory	576:584	arg1	pathway					586:592	the secretory pathway	572:592	the secretory pathway	572:592	Here, we observe that CA10, a ligand to neurexin in the secretory pathway, regulates neurexin-HS formation.					
33586859	2	6	from	Diversification	191:205	arg1	isoforms					232:239	numerous isoforms	223:239	numerous isoforms	223:239	Diversification of neurexins in numerous isoforms is believed to confer synapse-specific properties by engaging with distinct ligands.					
33586859	5	7	theme	non-HS	657:662	arg1	neurexin					664:671	non-HS neurexin	657:671	non-HS neurexin	657:671	CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS.					
33586859	7	8	from	residues	1020:1027	arg1	side					1039:1042	either side	1032:1042	either side of the serine	1032:1056	NMR reveals that CA10 engages residues on either side of the serine that can be HS-modified, suggesting that CA10 sterically blocks xylosyltransferase access in Golgi.					
33586859	6	9	theme	residue	981:987	arg1	xylosylation					954:965	xylosylation	954:965	the primary step of HS biosynthesis: xylosylation of the serine residue	917:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	3	10	theme	neurexin	351:358	arg1	molecules					360:368	neurexin molecules	351:368	neurexin molecules	351:368	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	7	11	from	access	1141:1146	arg1	Golgi					1151:1155	Golgi	1151:1155	Golgi	1151:1155	NMR reveals that CA10 engages residues on either side of the serine that can be HS-modified, suggesting that CA10 sterically blocks xylosyltransferase access in Golgi.					
33586859	3	12	theme	ligand	431:436	arg1	binding					438:444	ligand binding	431:444	ligand binding	431:444	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	3	13	theme	molecules	360:368	arg1	subset					341:346	a subset	339:346	a subset of neurexin molecules	339:368	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	6	14	theme	direct	861:866	arg1	interaction					868:878	a direct interaction	859:878	a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue	859:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	4	15	from	ligand	550:555	arg1	pathway					586:592	the secretory pathway	572:592	the secretory pathway	572:592	Here, we observe that CA10, a ligand to neurexin in the secretory pathway, regulates neurexin-HS formation.					
33586859	5	16	theme	HS	729:730	arg1	addition					732:739	HS addition	729:739	HS addition	729:739	CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS.					
33586859	1	17	theme	synapses	181:188	arg1	composition					166:176	the molecular composition	152:176	the molecular composition of synapses	152:188	Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses.					
33586859	3	18	theme	HS	395:396	arg1	glycosaminoglycan					399:415	a heparan sulfate (HS) glycosaminoglycan	376:415	a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled	376:504	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	7	19	theme	serine	1051:1056	arg1	side					1039:1042	either side	1032:1042	either side of the serine	1032:1056	NMR reveals that CA10 engages residues on either side of the serine that can be HS-modified, suggesting that CA10 sterically blocks xylosyltransferase access in Golgi.					
33586859	8	20	theme	HS	1214:1215	arg1	regulation					1200:1209	the regulation	1196:1209	the regulation of HS on neurexins	1196:1228	These results suggest a mechanism for the regulation of HS on neurexins and exemplify a new mechanism to regulate site-specific glycosylations.					
33586859	3	21	theme	heparan	378:384	arg1	glycosaminoglycan					399:415	a heparan sulfate (HS) glycosaminoglycan	376:415	a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled	376:504	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	6	22	theme	biosynthesis	940:951	arg1	step					929:932	the primary step	917:932	the primary step of HS biosynthesis: xylosylation of the serine residue	917:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	3	23	theme	post-translational	460:477	arg1	modification					479:490	this post-translational modification	455:490	this post-translational modification	455:490	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	3	24	theme	sulfate	386:392	arg1	glycosaminoglycan					399:415	a heparan sulfate (HS) glycosaminoglycan	376:415	a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled	376:504	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	6	25	theme	HS	937:938	arg1	biosynthesis					940:951	HS biosynthesis	937:951	HS biosynthesis	937:951	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	8	26	theme	new	1246:1248	arg1	mechanism					1250:1258	a new mechanism	1244:1258	a new mechanism to regulate site-specific glycosylations	1244:1299	These results suggest a mechanism for the regulation of HS on neurexins and exemplify a new mechanism to regulate site-specific glycosylations.					
33586859	0	27	theme	sulfate	32:38	arg1	addition					40:47	neurexin heparan sulfate addition	15:47	neurexin heparan sulfate addition	15:47	CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.					
33586859	5	28	theme	synapse	774:780	arg1	formation					782:790	synapse formation	774:790	synapse formation	774:790	CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS.					
33586859	0	29	from	binding	62:68	arg1	pathway					87:93	the secretory pathway	73:93	the secretory pathway	73:93	CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.					
33586859	0	30	theme	heparan	24:30	arg1	sulfate					32:38	neurexin heparan sulfate	15:38	neurexin heparan sulfate addition	15:47	CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.					
33586859	6	31	from	interaction	868:878	arg1	pathway					897:903	the secretory pathway	883:903	the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue	883:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	6	32	theme	primary	921:927	arg1	step					929:932	the primary step	917:932	the primary step of HS biosynthesis: xylosylation of the serine residue	917:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	2	33	theme	numerous	223:230	arg1	isoforms					232:239	numerous isoforms	223:239	numerous isoforms	223:239	Diversification of neurexins in numerous isoforms is believed to confer synapse-specific properties by engaging with distinct ligands.					
33586859	1	34	theme	presynaptic	110:120	arg1	molecules					131:139	presynaptic adhesion molecules	110:139	presynaptic adhesion molecules that shape the molecular composition of synapses	110:188	Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses.					
33586859	1	34	theme	presynaptic	110:120	arg1	Neurexins					96:104	Neurexins	96:104	Neurexins	96:104	Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses.					
33586859	5	35	located	found	648:652	arg1	neurexin					664:671	non-HS neurexin	657:671	non-HS neurexin	657:671	CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS.					
33586859	5	35	located	found	648:652	arg2	CA10					628:631	CA10	628:631	CA10	628:631	CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS.					
33586859	7	36	theme	xylosyltransferase	1122:1139	arg1	access					1141:1146	xylosyltransferase access	1122:1146	xylosyltransferase access in Golgi	1122:1155	NMR reveals that CA10 engages residues on either side of the serine that can be HS-modified, suggesting that CA10 sterically blocks xylosyltransferase access in Golgi.					
33586859	3	37	dep	carry	370:374	arg1	known					513:517	known	513:517	carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known	370:517	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	2	38	theme	neurexins	210:218	arg1	Diversification					191:205	Diversification	191:205	Diversification of neurexins in numerous isoforms	191:239	Diversification of neurexins in numerous isoforms is believed to confer synapse-specific properties by engaging with distinct ligands.					
33586859	3	39	contain	carry	370:374	arg1	subset					341:346	a subset	339:346	a subset of neurexin molecules	339:368	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	3	39	contain	carry	370:374	arg2	glycosaminoglycan					399:415	a heparan sulfate (HS) glycosaminoglycan	376:415	a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled	376:504	For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this post-translational modification is controlled is not known.					
33586859	1	40	theme	adhesion	122:129	arg1	molecules					131:139	presynaptic adhesion molecules	110:139	presynaptic adhesion molecules that shape the molecular composition of synapses	110:188	Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses.					
33586859	1	40	theme	adhesion	122:129	arg1	Neurexins					96:104	Neurexins	96:104	Neurexins	96:104	Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses.					
33586859	8	41	from	regulation	1200:1209	arg1	neurexins					1220:1228	neurexins	1220:1228	neurexins	1220:1228	These results suggest a mechanism for the regulation of HS on neurexins and exemplify a new mechanism to regulate site-specific glycosylations.					
33586859	2	42	theme	synapse-specific	263:278	arg1	properties					280:289	synapse-specific properties	263:289	synapse-specific properties	263:289	Diversification of neurexins in numerous isoforms is believed to confer synapse-specific properties by engaging with distinct ligands.					
33586859	6	43	dep	step	929:932	arg1	xylosylation					954:965	xylosylation	954:965	the primary step of HS biosynthesis: xylosylation of the serine residue	917:987	This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue.					
33586859	4	44	theme	neurexin-HS	605:615	arg1	formation					617:625	neurexin-HS formation	605:625	neurexin-HS formation	605:625	Here, we observe that CA10, a ligand to neurexin in the secretory pathway, regulates neurexin-HS formation.					
33586859	0	45	theme	direct	55:60	arg1	binding					62:68	a direct binding	53:68	a direct binding in the secretory pathway	53:93	CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.					
33586859	0	46	theme	neurexin	15:22	arg1	sulfate					32:38	neurexin heparan sulfate	15:38	neurexin heparan sulfate addition	15:47	CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.					
33586859	8	47	theme	site-specific	1272:1284	arg1	glycosylations					1286:1299	site-specific glycosylations	1272:1299	site-specific glycosylations	1272:1299	These results suggest a mechanism for the regulation of HS on neurexins and exemplify a new mechanism to regulate site-specific glycosylations.					
33586859	0	48	theme	secretory	77:85	arg1	pathway					87:93	the secretory pathway	73:93	the secretory pathway	73:93	CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.					
32919540	0	0	theme	fibers	91:96	arg1	profile					57:63	the digestive viscosity profile	33:63	the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer	33:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	1	1	theme	effects	330:336	arg1	predictive					297:306	predictive	297:306	predictive	297:306	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	1	1	theme	effects	330:336	arg1	method					158:163	A standard method	147:163	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer	147:258	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	3	2	theme	cooked	507:512	arg1	products					521:528	twelve uncooked and cooked cereal products	487:528	twelve uncooked and cooked cereal products	487:528	Digestograms of twelve uncooked and cooked cereal products were generated and data quality was assessed.					
32919540	0	3	theme	dietary	83:89	arg1	fibers					91:96	cereal soluble dietary fibers	68:96	cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer	68:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	3	4	theme	cereal	514:519	arg1	products					521:528	twelve uncooked and cooked cereal products	487:528	twelve uncooked and cooked cereal products	487:528	Digestograms of twelve uncooked and cooked cereal products were generated and data quality was assessed.					
32919540	1	5	theme	Rapid	240:244	arg1	ViscoAnalyzer					246:258	a Rapid ViscoAnalyzer	238:258	a Rapid ViscoAnalyzer	238:258	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	0	6	theme	in	104:105	arg1	digestion					113:121	in vitro digestion	104:121	in vitro digestion in Rapid ViscoAnalyzer	104:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	1	7	theme	cereal	341:346	arg1	foods					348:352	cereal foods	341:352	cereal foods	341:352	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	6	8	theme	fibers	1181:1186	arg1	effects					1154:1160	the biological effects	1139:1160	the biological effects of soluble dietary fibers in cereal products and similar products	1139:1226	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	3	9	theme	data	549:552	arg1	quality					554:560	data quality	549:560	data quality	549:560	Digestograms of twelve uncooked and cooked cereal products were generated and data quality was assessed.					
32919540	5	10	theme	decreasing	946:955	arg1	η1					920:921	η1	920:921	η1	920:921	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	10	theme	decreasing	946:955	arg1	viscosity					957:965	the decreasing viscosity	942:965	the decreasing viscosity of digestible polymeric nutrients	942:999	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	1	11	theme	foods	348:352	arg1	effects					330:336	some physiological effects	311:336	some physiological effects of cereal foods	311:352	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	6	12	theme	cereal	1191:1196	arg1	products					1198:1205	cereal products	1191:1205	cereal products	1191:1205	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	5	13	theme	digestograms	859:870	arg1	interpretation					837:850	interpretation	837:850	interpretation of the digestograms	837:870	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	14	theme	products	910:917	arg1	composition					891:901	the composition	887:901	the composition of the products	887:917	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	0	15	dep	in	104:105	arg1	vitro					107:111	vitro	107:111	vitro	107:111	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	2	16	theme	mathematical	384:395	arg1	model					397:401	a simple mathematical model	375:401	a simple mathematical model to analyze quantitatively the digestograms obtained by that method	375:468	This paper proposes a simple mathematical model to analyze quantitatively the digestograms obtained by that method.					
32919540	4	17	dep	[Formula	673:680	arg1	see					683:685	see	683:685	see text	683:690	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	2	18	theme	simple	377:382	arg1	model					397:401	a simple mathematical model	375:401	a simple mathematical model to analyze quantitatively the digestograms obtained by that method	375:468	This paper proposes a simple mathematical model to analyze quantitatively the digestograms obtained by that method.					
32919540	6	19	theme	dietary	1173:1179	arg1	fibers					1181:1186	soluble dietary fibers	1165:1186	soluble dietary fibers	1165:1186	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	0	20	theme	Quantitative	0:11	arg1	characterization					13:28	Quantitative characterization	0:28	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.	0:145	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	5	21	theme	dietary	1068:1074	arg1	fibers					1076:1081	viscous dietary fibers	1060:1081	viscous dietary fibers	1060:1081	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	6	22	theme	soluble	1165:1171	arg1	fibers					1181:1186	soluble dietary fibers	1165:1186	soluble dietary fibers	1165:1186	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	5	23	theme	experimental	807:818	arg1	data					820:823	experimental data	807:823	experimental data	807:823	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	0	24	theme	Rapid	126:130	arg1	ViscoAnalyzer					132:144	Rapid ViscoAnalyzer	126:144	Rapid ViscoAnalyzer	126:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	6	25	from	effects	1154:1160	arg1	products					1219:1226	similar products	1211:1226	similar products	1211:1226	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	6	25	from	effects	1154:1160	arg1	products					1198:1205	cereal products	1191:1205	cereal products	1191:1205	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	4	26	theme	Experimental	576:587	arg1	data					589:592	Experimental data	576:592	Experimental data	576:592	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	4	27	theme	viscosity	613:621	arg1	ηmodel=η1+η2					629:640	a viscosity model ηmodel=η1+η2	611:640	a viscosity model ηmodel=η1+η2	611:640	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	5	28	theme	fibers	1076:1081	arg1	η2					1009:1010	η2	1009:1010	η2	1009:1010	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	28	theme	fibers	1076:1081	arg1	development					1045:1055	the viscosity development	1031:1055	the viscosity development of viscous dietary fibers	1031:1081	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	4	29	theme	viscosity	734:742	arg1	increase					744:751	viscosity increase	734:751	viscosity increase	734:751	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	1	30	theme	cereal	192:197	arg1	products					199:206	cereal products	192:206	cereal products	192:206	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	0	31	theme	viscosity	47:55	arg1	profile					57:63	the digestive viscosity profile	33:63	the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer	33:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	1	32	theme	products	199:206	arg1	η					186:186	η	186:186	η	186:186	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	1	32	theme	products	199:206	arg1	viscosity					175:183	viscosity	175:183	viscosity (η) of cereal products	175:206	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	0	33	theme	digestive	37:45	arg1	profile					57:63	the digestive viscosity profile	33:63	the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer	33:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	1	34	dep	in	216:217	arg1	vitro					219:223	vitro	219:223	vitro	219:223	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	4	35	dep	[Formula	649:656	arg1	see					659:661	see	659:661	see text	659:666	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	5	36	theme	good	787:790	arg1	agreement					792:800	very good agreement	782:800	very good agreement	782:800	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	1	37	from	digestion	225:233	arg1	ViscoAnalyzer					246:258	a Rapid ViscoAnalyzer	238:258	a Rapid ViscoAnalyzer	238:258	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	3	38	theme	uncooked	494:501	arg1	products					521:528	twelve uncooked and cooked cereal products	487:528	twelve uncooked and cooked cereal products	487:528	Digestograms of twelve uncooked and cooked cereal products were generated and data quality was assessed.					
32919540	0	39	theme	profile	57:63	arg1	characterization					13:28	Quantitative characterization	0:28	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.	0:145	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	0	40	from	digestion	113:121	arg1	ViscoAnalyzer					132:144	Rapid ViscoAnalyzer	126:144	Rapid ViscoAnalyzer	126:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	5	41	theme	viscosity	1035:1043	arg1	η2					1009:1010	η2	1009:1010	η2	1009:1010	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	41	theme	viscosity	1035:1043	arg1	development					1045:1055	the viscosity development	1031:1055	the viscosity development of viscous dietary fibers	1031:1081	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	42	theme	nutrients	991:999	arg1	η1					920:921	η1	920:921	η1	920:921	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	42	theme	nutrients	991:999	arg1	viscosity					957:965	the decreasing viscosity	942:965	the decreasing viscosity of digestible polymeric nutrients	942:999	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	4	43	theme	viscosity	711:719	arg1	decrease					721:728	viscosity decrease	711:728	viscosity decrease	711:728	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	1	44	theme	in	216:217	arg1	digestion					225:233	in vitro digestion	216:233	in vitro digestion in a Rapid ViscoAnalyzer	216:258	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	5	45	theme	polymeric	981:989	arg1	nutrients					991:999	digestible polymeric nutrients	970:999	digestible polymeric nutrients	970:999	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	5	46	theme	viscous	1060:1066	arg1	fibers					1076:1081	viscous dietary fibers	1060:1081	viscous dietary fibers	1060:1081	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	4	47	dep	decrease	721:728	arg1	components					753:762	components	753:762	components	753:762	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
32919540	6	48	theme	biological	1143:1152	arg1	effects					1154:1160	the biological effects	1139:1160	the biological effects of soluble dietary fibers in cereal products and similar products	1139:1226	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	0	49	theme	soluble	75:81	arg1	fibers					91:96	cereal soluble dietary fibers	68:96	cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer	68:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	5	50	theme	digestible	970:979	arg1	nutrients					991:999	digestible polymeric nutrients	970:999	digestible polymeric nutrients	970:999	The model showed very good agreement with experimental data and enabled interpretation of the digestograms in relation to the composition of the products: η1 was interpreted as the decreasing viscosity of digestible polymeric nutrients whereas η2 was interpreted as the viscosity development of viscous dietary fibers.					
32919540	6	51	theme	similar	1211:1217	arg1	products					1219:1226	similar products	1211:1226	similar products	1211:1226	This model may be useful to investigate quantitatively the biological effects of soluble dietary fibers in cereal products and similar products.					
32919540	3	52	theme	products	521:528	arg1	Digestograms					471:482	Digestograms	471:482	Digestograms of twelve uncooked and cooked cereal products	471:528	Digestograms of twelve uncooked and cooked cereal products were generated and data quality was assessed.					
32919540	0	53	theme	cereal	68:73	arg1	fibers					91:96	cereal soluble dietary fibers	68:96	cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer	68:144	Quantitative characterization of the digestive viscosity profile of cereal soluble dietary fibers using in vitro digestion in Rapid ViscoAnalyzer.					
32919540	1	54	theme	physiological	316:328	arg1	effects					330:336	some physiological effects	311:336	some physiological effects of cereal foods	311:352	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	1	55	theme	standard	149:156	arg1	predictive					297:306	predictive	297:306	predictive	297:306	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	1	55	theme	standard	149:156	arg1	method					158:163	A standard method	147:163	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer	147:258	A standard method measuring viscosity (η) of cereal products through in vitro digestion in a Rapid ViscoAnalyzer has been developed previously and is predictive of some physiological effects of cereal foods.					
32919540	4	56	theme	model	623:627	arg1	ηmodel=η1+η2					629:640	a viscosity model ηmodel=η1+η2	611:640	a viscosity model ηmodel=η1+η2	611:640	Experimental data were fitted with a viscosity model ηmodel=η1+η2, where [Formula: see text] and [Formula: see text] were respectively viscosity decrease and viscosity increase components.					
34175336	1	0	theme	honeycomb-like	365:378	arg1	DP					408:409	DP	408:409	DP	408:409	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	1	0	theme	honeycomb-like	365:378	arg1	micro-framework					391:405	wood-based honeycomb-like celluloses micro-framework	354:405	wood-based honeycomb-like celluloses micro-framework (DP)	354:410	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	2	1	theme	heat	585:588	arg1	conduction					590:599	heat conduction	585:599	heat conduction	585:599	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	8	2	theme	actual	1416:1421	arg1	storage					1435:1441	actual latent heat storage	1416:1441	actual latent heat storage	1416:1441	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	2	theme	actual	1416:1421	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	5	3	theme	physical	848:855	arg1	interactions					857:868	physical interactions	848:868	physical interactions between the components of FPCMs	848:900	FTIR and XRD analysis show that there is only a combination of physical interactions between the components of FPCMs.					
34175336	1	4	theme	electrical	284:293	arg1	response					295:302	thermal and electrical response	272:302	thermal and electrical response	272:302	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	0	5	theme	thermal	106:112	arg1	response					129:136	thermal and electrical response	106:136	thermal and electrical response	106:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	1	6	theme	celluloses	380:389	arg1	DP					408:409	DP	408:409	DP	408:409	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	1	6	theme	celluloses	380:389	arg1	micro-framework					391:405	wood-based honeycomb-like celluloses micro-framework	354:405	wood-based honeycomb-like celluloses micro-framework (DP)	354:410	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	5	7	theme	interactions	857:868	arg1	combination					833:843	only a combination	826:843	only a combination of physical interactions between the components of FPCMs	826:900	FTIR and XRD analysis show that there is only a combination of physical interactions between the components of FPCMs.					
34175336	8	8	theme	sand	1588:1591	arg1	response					1604:1611	thermal storage sand electrical response	1572:1611	thermal storage sand electrical response	1572:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	2	9	theme	skeleton	514:521	arg1	structure					523:531	a skeleton structure	512:531	a skeleton structure to seal E7U3 and E7T3	512:553	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	2	9	theme	skeleton	514:521	arg1	DP					501:502	DP	501:502	DP	501:502	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	2	9	theme	skeleton	514:521	arg1	pathways					572:579	more pathways	567:579	more pathways for heat conduction	567:599	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	1	10	dep	DP-E7U3-CNT	312:322	arg1	DP-E7T3-CNT					325:335	DP-E7T3-CNT	325:335	DP-E7T3-CNT	325:335	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	8	11	theme	storage	1580:1586	arg1	response					1604:1611	thermal storage sand electrical response	1572:1611	thermal storage sand electrical response	1572:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	12	theme	light-heat	1378:1387	arg1	storage					1435:1441	actual latent heat storage	1416:1441	actual latent heat storage	1416:1441	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	12	theme	light-heat	1378:1387	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	12	theme	light-heat	1378:1387	arg1	performance					1488:1498	thermal and electrical response performance	1456:1498	thermal and electrical response performance	1456:1498	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	12	theme	light-heat	1378:1387	arg1	release					1447:1453	release	1447:1453	release	1447:1453	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	0	13	theme	electrical	118:127	arg1	response					129:136	thermal and electrical response	106:136	thermal and electrical response	106:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	2	14	theme	more	567:570	arg1	structure					523:531	a skeleton structure	512:531	a skeleton structure to seal E7U3 and E7T3	512:553	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	2	14	theme	more	567:570	arg1	DP					501:502	DP	501:502	DP	501:502	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	2	14	theme	more	567:570	arg1	pathways					572:579	more pathways	567:579	more pathways for heat conduction	567:599	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	8	15	theme	response	1479:1486	arg1	performance					1488:1498	thermal and electrical response performance	1456:1498	thermal and electrical response performance	1456:1498	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	15	theme	response	1479:1486	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	0	16	with	materials	91:99	arg1	response					129:136	thermal and electrical response	106:136	thermal and electrical response	106:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	6	17	theme	thermal	1116:1122	arg1	conductivity					1124:1135	thermal conductivity	1116:1135	thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K))	1116:1168	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	18	theme	DP-E7U3-1.5CNT	966:979	arg1	nanotubes					1033:1041	DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes	966:1041	DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%)	966:1051	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	18	theme	DP-E7U3-1.5CNT	966:979	arg1	%					1050:1050	1.5 wt%	1044:1050	1.5 wt%	1044:1050	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	8	19	with	materials	1557:1565	arg1	response					1604:1611	thermal storage sand electrical response	1572:1611	thermal storage sand electrical response	1572:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	7	20	theme	high	1218:1221	arg1	retention					1235:1243	high latent heat retention	1218:1243	high latent heat retention	1218:1243	Both DP-E7U3-1.5CNT and DP-E7T3-1.5CNT exhibit high latent heat retention and thermal stability after 100 heating-cooling cycles.					
34175336	5	21	theme	FPCMs	896:900	arg1	components					882:891	the components	878:891	the components of FPCMs	878:900	FTIR and XRD analysis show that there is only a combination of physical interactions between the components of FPCMs.					
34175336	3	22	theme	thermal	657:663	arg1	conductivity					665:676	thermal conductivity	657:676	thermal conductivity	657:676	The CNT acts as an extended surface to further improve thermal conductivity.					
34175336	6	23	theme	analysis	939:946	arg1	results					948:954	thermal conductivity analysis results	918:954	thermal conductivity analysis results	918:954	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	8	24	dep	make	1507:1510	arg1	has					1515:1517	has	1515:1517	make it has great potential to be multifunctional materials with thermal storage sand electrical response	1507:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	4	25	theme	pore	717:720	arg1	structure					722:730	the honeycomb-like pore structure	698:730	the honeycomb-like pore structure of DP	698:736	FE-SEM showed that the honeycomb-like pore structure of DP was completely filled with E7U3, E7T3 and CNT.					
34175336	7	26	theme	thermal	1249:1255	arg1	stability					1257:1265	thermal stability	1249:1265	thermal stability	1249:1265	Both DP-E7U3-1.5CNT and DP-E7T3-1.5CNT exhibit high latent heat retention and thermal stability after 100 heating-cooling cycles.					
34175336	0	27	theme	cellulose	27:35	arg1	materials					91:99	wood-based cellulose micro-framework composite form-stable multifunctional materials	16:99	wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response	16:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	1	28	theme	DP-E7U3-CNT	312:322	arg1	FPCMs					305:309	FPCMs	305:309	FPCMs: DP-E7U3-CNT, DP-E7T3-CNT	305:335	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	6	29	dep	conductivity	1124:1135	arg1	0.9363 W/					1154:1162	0.9363 W/	1154:1162	0.9363 W/(m·K)	1154:1167	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	29	dep	conductivity	1124:1135	arg1	0.9832 W/					1138:1146	0.9832 W/(m·K)	1138:1151	0.9832 W/(m·K)	1138:1151	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	4	30	theme	DP	735:736	arg1	structure					722:730	the honeycomb-like pore structure	698:730	the honeycomb-like pore structure of DP	698:736	FE-SEM showed that the honeycomb-like pore structure of DP was completely filled with E7U3, E7T3 and CNT.					
34175336	8	31	theme	thermal	1456:1462	arg1	performance					1488:1498	thermal and electrical response performance	1456:1498	thermal and electrical response performance	1456:1498	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	31	theme	thermal	1456:1462	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	5	32	theme	XRD	794:796	arg1	analysis					798:805	XRD analysis	794:805	XRD analysis	794:805	FTIR and XRD analysis show that there is only a combination of physical interactions between the components of FPCMs.					
34175336	0	33	theme	wood-based	16:25	arg1	materials					91:99	wood-based cellulose micro-framework composite form-stable multifunctional materials	16:99	wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response	16:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	6	34	theme	thermal	918:924	arg1	results					948:954	thermal conductivity analysis results	918:954	thermal conductivity analysis results	918:954	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	2	35	theme	seal	536:539	arg1	E7U3					541:544	E7U3	541:544	E7U3	541:544	In FPCMs, DP acts as a skeleton structure to seal E7U3 and E7T3 and provide more pathways for heat conduction.					
34175336	1	36	theme	carbon	413:418	arg1	CNT					431:433	CNT	431:433	CNT	431:433	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	1	36	theme	carbon	413:418	arg1	nanotubes					420:428	carbon nanotubes	413:428	carbon nanotubes (CNT)	413:434	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	8	37	theme	great	1519:1523	arg1	potential					1525:1533	great potential	1519:1533	great potential to be multifunctional materials with thermal storage sand electrical response	1519:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	0	38	theme	composite	53:61	arg1	materials					91:99	wood-based cellulose micro-framework composite form-stable multifunctional materials	16:99	wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response	16:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	7	39	theme	latent	1223:1228	arg1	retention					1235:1243	high latent heat retention	1218:1243	high latent heat retention	1218:1243	Both DP-E7U3-1.5CNT and DP-E7T3-1.5CNT exhibit high latent heat retention and thermal stability after 100 heating-cooling cycles.					
34175336	3	40	theme	extended	621:628	arg1	surface					630:636	an extended surface	618:636	an extended surface	618:636	The CNT acts as an extended surface to further improve thermal conductivity.					
34175336	3	40	theme	extended	621:628	arg1	CNT					606:608	The CNT	602:608	The CNT	602:608	The CNT acts as an extended surface to further improve thermal conductivity.					
34175336	6	41	with	DP-E7T3-1.5CNT	985:998	arg1	fraction					1014:1021	the mass fraction	1005:1021	the mass fraction of carbon	1005:1031	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	8	42	from	performance	1363:1373	arg1	storage					1435:1441	actual latent heat storage	1416:1441	actual latent heat storage	1416:1441	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	42	from	performance	1363:1373	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	42	from	performance	1363:1373	arg1	performance					1488:1498	thermal and electrical response performance	1456:1498	thermal and electrical response performance	1456:1498	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	42	from	performance	1363:1373	arg1	release					1447:1453	release	1447:1453	release	1447:1453	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	1	43	theme	form-stable	229:239	arg1	materials					257:265	form-stable multifunctional materials	229:265	form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT)	229:336	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	0	44	theme	micro-framework	37:51	arg1	materials					91:99	wood-based cellulose micro-framework composite form-stable multifunctional materials	16:99	wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response	16:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	1	45	dep	materials	257:265	arg1	FPCMs					305:309	FPCMs	305:309	FPCMs: DP-E7U3-CNT, DP-E7T3-CNT	305:335	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	6	46	theme	DP-E7T3-1.5CNT	985:998	arg1	nanotubes					1033:1041	DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes	966:1041	DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%)	966:1051	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	46	theme	DP-E7T3-1.5CNT	985:998	arg1	%					1050:1050	1.5 wt%	1044:1050	1.5 wt%	1044:1050	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	1	47	theme	multifunctional	241:255	arg1	materials					257:265	form-stable multifunctional materials	229:265	form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT)	229:336	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	0	48	theme	multifunctional	75:89	arg1	materials					91:99	wood-based cellulose micro-framework composite form-stable multifunctional materials	16:99	wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response	16:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	6	49	theme	DSC	903:905	arg1	curves					907:912	DSC curves	903:912	DSC curves	903:912	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	50	theme	conductivity	926:937	arg1	results					948:954	thermal conductivity analysis results	918:954	thermal conductivity analysis results	918:954	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	8	51	theme	excellent	1353:1361	arg1	performance					1363:1373	excellent performance	1353:1373	excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response	1353:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	0	52	theme	form-stable	63:73	arg1	materials					91:99	wood-based cellulose micro-framework composite form-stable multifunctional materials	16:99	wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response	16:136	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	8	53	theme	multifunctional	1541:1555	arg1	materials					1557:1565	multifunctional materials	1541:1565	multifunctional materials with thermal storage sand electrical response	1541:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	0	54	theme	-carbon	182:188	arg1	nanotubes					190:198	erythritol-urea (thiourea)-carbon nanotubes	156:198	erythritol-urea (thiourea)-carbon nanotubes	156:198	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	8	55	theme	heat	1430:1433	arg1	storage					1435:1441	actual latent heat storage	1416:1441	actual latent heat storage	1416:1441	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	55	theme	heat	1430:1433	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	56	theme	latent	1423:1428	arg1	storage					1435:1441	actual latent heat storage	1416:1441	actual latent heat storage	1416:1441	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	56	theme	latent	1423:1428	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	7	57	theme	heating-cooling	1277:1291	arg1	cycles					1293:1298	100 heating-cooling cycles	1273:1298	100 heating-cooling cycles	1273:1298	Both DP-E7U3-1.5CNT and DP-E7T3-1.5CNT exhibit high latent heat retention and thermal stability after 100 heating-cooling cycles.					
34175336	6	58	contain	have	1053:1056	arg2	values					1082:1087	the highest latent heat values	1058:1087	the highest latent heat values (230.3 J/g, 272.2 J/g)	1058:1110	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	58	contain	have	1053:1056	arg2	conductivity					1124:1135	thermal conductivity	1116:1135	thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K))	1116:1168	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	58	contain	have	1053:1056	arg1	nanotubes					1033:1041	DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes	966:1041	DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%)	966:1051	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	58	contain	have	1053:1056	arg1	%					1050:1050	1.5 wt%	1044:1050	1.5 wt%	1044:1050	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	4	59	theme	honeycomb-like	702:715	arg1	structure					722:730	the honeycomb-like pore structure	698:730	the honeycomb-like pore structure of DP	698:736	FE-SEM showed that the honeycomb-like pore structure of DP was completely filled with E7U3, E7T3 and CNT.					
34175336	1	60	theme	materials	257:265	arg1	kinds					220:224	two kinds	216:224	two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT)	216:336	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	6	61	theme	latent	1070:1075	arg1	values					1082:1087	the highest latent heat values	1058:1087	the highest latent heat values (230.3 J/g, 272.2 J/g)	1058:1110	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	62	theme	heat	1077:1080	arg1	values					1082:1087	the highest latent heat values	1058:1087	the highest latent heat values (230.3 J/g, 272.2 J/g)	1058:1110	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	8	63	theme	electrical	1468:1477	arg1	performance					1488:1498	thermal and electrical response performance	1456:1498	thermal and electrical response performance	1456:1498	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	63	theme	electrical	1468:1477	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	6	64	theme	mass	1009:1012	arg1	fraction					1014:1021	the mass fraction	1005:1021	the mass fraction of carbon	1005:1031	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	8	65	theme	energy	1389:1394	arg1	storage					1435:1441	actual latent heat storage	1416:1441	actual latent heat storage	1416:1441	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	65	theme	energy	1389:1394	arg1	conversion-storage					1396:1413	light-heat energy conversion-storage	1378:1413	light-heat energy conversion-storage	1378:1413	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	65	theme	energy	1389:1394	arg1	performance					1488:1498	thermal and electrical response performance	1456:1498	thermal and electrical response performance	1456:1498	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	65	theme	energy	1389:1394	arg1	release					1447:1453	release	1447:1453	release	1447:1453	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	6	66	theme	carbon	1026:1031	arg1	fraction					1014:1021	the mass fraction	1005:1021	the mass fraction of carbon	1005:1031	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	67	theme	highest	1062:1068	arg1	values					1082:1087	the highest latent heat values	1058:1087	the highest latent heat values (230.3 J/g, 272.2 J/g)	1058:1110	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	7	68	theme	heat	1230:1233	arg1	retention					1235:1243	high latent heat retention	1218:1243	high latent heat retention	1218:1243	Both DP-E7U3-1.5CNT and DP-E7T3-1.5CNT exhibit high latent heat retention and thermal stability after 100 heating-cooling cycles.					
34175336	1	69	theme	thermal	272:278	arg1	response					295:302	thermal and electrical response	272:302	thermal and electrical response	272:302	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	0	70	theme	materials	91:99	arg1	Construction					0:11	Construction	0:11	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.	0:199	Construction of wood-based cellulose micro-framework composite form-stable multifunctional materials with thermal and electrical response via incorporating erythritol-urea (thiourea)-carbon nanotubes.					
34175336	1	71	theme	wood-based	354:363	arg1	DP					408:409	DP	408:409	DP	408:409	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	1	71	theme	wood-based	354:363	arg1	micro-framework					391:405	wood-based honeycomb-like celluloses micro-framework	354:405	wood-based honeycomb-like celluloses micro-framework (DP)	354:410	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	6	72	dep	values	1082:1087	arg1	272.2 J/g					1101:1109	272.2 J/g	1101:1109	272.2 J/g	1101:1109	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	6	72	dep	values	1082:1087	arg1	230.3 J/g					1090:1098	230.3 J/g	1090:1098	230.3 J/g	1090:1098	DSC curves and thermal conductivity analysis results show that DP-E7U3-1.5CNT and DP-E7T3-1.5CNT with the mass fraction of carbon nanotubes (1.5 wt%) have the highest latent heat values (230.3 J/g, 272.2 J/g) and thermal conductivity (0.9832 W/(m·K), 0.9363 W/(m·K)).					
34175336	1	73	with	materials	257:265	arg1	response					295:302	thermal and electrical response	272:302	thermal and electrical response	272:302	In this study, two kinds of form-stable multifunctional materials with thermal and electrical response (FPCMs: DP-E7U3-CNT, DP-E7T3-CNT) are composed of wood-based honeycomb-like celluloses micro-framework (DP), carbon nanotubes (CNT), erythritol-urea (E7U3) or erythritol-thiourea (E7T3).					
34175336	8	74	theme	electrical	1593:1602	arg1	response					1604:1611	thermal storage sand electrical response	1572:1611	thermal storage sand electrical response	1572:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
34175336	8	75	theme	thermal	1572:1578	arg1	response					1604:1611	thermal storage sand electrical response	1572:1611	thermal storage sand electrical response	1572:1611	In addition, DP-E7U3-1.5CNT and DP-E7T3-1.5CNT show excellent performance in light-heat energy conversion-storage, actual latent heat storage and release, thermal and electrical response performance, which make it has great potential to be multifunctional materials with thermal storage sand electrical response.					
32369957	4	0	theme	gut	1123:1125	arg1	composition					1138:1148	gut microbiota composition	1123:1148	gut microbiota composition	1123:1148	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	2	1	theme	intervention	528:539	arg1	effect					512:517	the effect	508:517	the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice	508:603	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	4	2	dep	reduction	1057:1065	arg1	p					1068:1068	p < 0.001	1068:1076	p < 0.001	1068:1076	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	6	3	theme	foodborne	1442:1450	arg1	pathogen					1452:1459	foodborne pathogen	1442:1459	foodborne pathogen	1442:1459	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	2	4	theme	2'-FL	522:526	arg1	intervention					528:539	2'-FL intervention	522:539	2'-FL intervention	522:539	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	2	5	theme	protective	578:587	arg1	effects					589:595	its protective effects	574:595	its protective effects on mice	574:603	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	2	6	from	model	418:422	arg1	mice					476:479	healthy adult mice	462:479	healthy adult mice	462:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	5	7	theme	bacteria	1364:1371	arg1	colonization					1340:1351	the colonization	1336:1351	the colonization of harmful bacteria	1336:1371	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	4	8	theme	composition	1138:1148	arg1	modulation					1109:1118	the modulation	1105:1118	the modulation of gut microbiota composition	1105:1148	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	3	9	theme	intestinal	745:754	arg1	inflammation					756:767	intestinal inflammation	745:767	intestinal inflammation	745:767	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	2	10	theme	infection	449:457	arg1	model					418:422	a model	416:422	a model of Escherichia coli O157 infection in healthy adult mice	416:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	3	11	theme	intestinal	839:848	arg1	function					858:865	intestinal barrier function	839:865	intestinal barrier function	839:865	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	4	12	dep	E.	1019:1020	arg1	coli					1022:1025	coli	1022:1025	coli	1022:1025	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	13	theme	beneficial	874:883	arg1	effects					885:891	These beneficial effects	868:891	These beneficial effects	868:891	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	3	14	theme	<	715:715	arg1	%					710:710	more than 90%	698:710	more than 90% (p < 0.001)	698:722	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	3	14	theme	<	715:715	arg1	p					713:713	p < 0.001	713:721	p < 0.001	713:721	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	2	15	theme	O157	444:447	arg1	infection					449:457	Escherichia coli O157 infection	427:457	Escherichia coli O157 infection in healthy adult mice	427:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	3	16	theme	E.	651:652	arg1	colonization					664:675	E. coli O157 colonization	651:675	E. coli O157 colonization	651:675	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	5	17	theme	fundamental	1258:1268	arg1	means					1270:1274	the fundamental means	1254:1274	the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria	1254:1371	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	6	18	theme	2'-FL	1415:1419	arg1	infections					1461:1470	2'-FL in the prevention of foodborne pathogen infections	1415:1470	2'-FL in the prevention of foodborne pathogen infections	1415:1470	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	6	19	from	prevention	1428:1437	arg1	2'-FL					1415:1419	2'-FL	1415:1419	2'-FL	1415:1419	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	3	20	theme	2'-FL	630:634	arg1	intake					636:641	2'-FL intake	630:641	2'-FL intake	630:641	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	2	21	theme	coli	547:550	arg1	O157					552:555	E. coli O157 colonization and its protective effects on mice	544:603	O157	552:555	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	4	22	theme	adhesion	1037:1044	arg1	inhibition					1005:1014	inhibition	1005:1014	inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	1005:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	22	theme	adhesion	1037:1044	arg1	MUC2					955:958	MUC2	955:958	MUC2 (increased by more than 20%, p < 0.001)	955:998	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	2	23	from	O157	552:555	arg1	mice					600:603	mice	600:603	mice	600:603	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	0	24	theme	Protective	4:13	arg1	Effects					15:21	The Protective Effects	0:21	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection	0:73	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	2	25	theme	E.	544:545	arg1	O157					552:555	E. coli O157 colonization and its protective effects on mice	544:603	O157	552:555	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	4	26	theme	cell	1032:1035	arg1	reduction					1057:1065	about 30% reduction	1047:1065	about 30% reduction	1047:1065	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	26	theme	cell	1032:1035	arg1	adhesion					1037:1044	E. coli O157 cell adhesion	1019:1044	E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	1019:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	27	theme	<	991:991	arg1	p					989:989	p < 0.001	989:997	p < 0.001	989:997	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	27	theme	<	991:991	arg1	%					986:986	more than 20%	974:986	more than 20%	974:986	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	3	28	dep	E.	651:652	arg1	coli					654:657	coli	654:657	coli	654:657	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	6	29	from	2'-FL	1415:1419	arg1	prevention					1428:1437	the prevention	1424:1437	the prevention of foodborne pathogen	1424:1459	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	6	30	theme	pathogen	1452:1459	arg1	prevention					1428:1437	the prevention	1424:1437	the prevention of foodborne pathogen	1424:1459	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	5	31	theme	probiotic	1223:1231	arg1	Akkermansia					1198:1208	Akkermansia	1198:1208	Akkermansia	1198:1208	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	5	31	theme	probiotic	1223:1231	arg1	potential					1213:1221	a potential probiotic	1211:1231	a potential probiotic	1211:1231	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	4	32	theme	<	1070:1070	arg1	p					1068:1068	p < 0.001	1068:1076	p < 0.001	1068:1076	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	3	33	theme	fatty	813:817	arg1	acids					819:823	fecal short-chain fatty acids	795:823	fecal short-chain fatty acids	795:823	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	1	34	theme	richest	172:178	arg1	colonization					270:281	the colonization	266:281	the colonization of harmful microbiota	266:303	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	1	34	theme	richest	172:178	arg1	component					180:188	the richest component	168:188	the richest component in human milk oligosaccharides (HMOs)	168:226	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	2	35	dep	O157	552:555	arg1	colonization					557:568	colonization	557:568	colonization	557:568	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	2	36	from	effect	512:517	arg1	effects					589:595	its protective effects	574:595	its protective effects on mice	574:603	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	2	36	from	effect	512:517	arg1	O157					552:555	E. coli O157 colonization and its protective effects on mice	544:603	O157	552:555	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	0	37	theme	Microbiota	113:122	arg1	Regulation					95:104	the Regulation	91:104	the Regulation of Gut Microbiota	91:122	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	0	37	theme	Microbiota	113:122	arg1	Inhibition					132:141	the Inhibition	128:141	the Inhibition of Pathogen Adhesion	128:162	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	4	38	dep	MUC2	955:958	arg1	increased					961:969	increased	961:969	increased by more than 20%, p < 0.001	961:997	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	3	39	theme	acids	819:823	arg1	content					784:790	the content	780:790	the content of fecal short-chain fatty acids	780:823	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	4	40	theme	increased	916:924	arg1	expression					926:935	the increased expression	912:935	the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	912:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	0	41	theme	Gut	109:111	arg1	Microbiota					113:122	Gut Microbiota	109:122	Gut Microbiota	109:122	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	1	42	theme	infection	340:348	arg1	risk					332:335	the risk	328:335	the risk of infection	328:348	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	3	43	theme	fecal	795:799	arg1	acids					819:823	fecal short-chain fatty acids	795:823	fecal short-chain fatty acids	795:823	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	6	44	from	use	1408:1410	arg1	human					1475:1479	human	1475:1479	human	1475:1479	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	3	45	theme	O157	659:662	arg1	colonization					664:675	E. coli O157 colonization	651:675	E. coli O157 colonization	651:675	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	2	46	theme	adult	470:474	arg1	mice					476:479	healthy adult mice	462:479	healthy adult mice	462:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	3	47	theme	short-chain	801:811	arg1	acids					819:823	fecal short-chain fatty acids	795:823	fecal short-chain fatty acids	795:823	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	1	48	theme	human	193:197	arg1	HMOs					222:225	HMOs	222:225	HMOs	222:225	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	1	48	theme	human	193:197	arg1	oligosaccharides					204:219	human milk oligosaccharides	193:219	human milk oligosaccharides (HMOs)	193:226	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	0	49	theme	2'-Fucosyllactose	26:42	arg1	Effects					15:21	The Protective Effects	0:21	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection	0:73	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	2	50	theme	healthy	462:468	arg1	mice					476:479	healthy adult mice	462:479	healthy adult mice	462:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	3	51	theme	mice	680:683	arg1	intestine					685:693	mice intestine	680:693	mice intestine	680:693	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	0	52	dep	E.	52:53	arg1	Coli					55:58	Coli	55:58	Coli	55:58	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	4	53	theme	E.	1019:1020	arg1	reduction					1057:1065	about 30% reduction	1047:1065	about 30% reduction	1047:1065	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	53	theme	E.	1019:1020	arg1	adhesion					1037:1044	E. coli O157 cell adhesion	1019:1044	E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	1019:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	1	54	theme	milk	199:202	arg1	HMOs					222:225	HMOs	222:225	HMOs	222:225	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	1	54	theme	milk	199:202	arg1	oligosaccharides					204:219	human milk oligosaccharides	193:219	human milk oligosaccharides (HMOs)	193:226	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	2	55	dep	Escherichia	427:437	arg1	coli					439:442	coli	439:442	coli	439:442	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	0	56	theme	E.	52:53	arg1	Infection					65:73	E. Coli O157 Infection	52:73	E. Coli O157 Infection	52:73	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	1	57	theme	harmful	286:292	arg1	microbiota					294:303	harmful microbiota	286:303	harmful microbiota	286:303	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	5	58	theme	mucin	1318:1322	arg1	expression					1304:1313	the expression	1300:1313	the expression of mucin	1300:1322	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	4	59	theme	%	1055:1055	arg1	reduction					1057:1065	about 30% reduction	1047:1065	about 30% reduction	1047:1065	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	59	theme	%	1055:1055	arg1	adhesion					1037:1044	E. coli O157 cell adhesion	1019:1044	E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	1019:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	1	60	from	component	180:188	arg1	HMOs					222:225	HMOs	222:225	HMOs	222:225	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	1	60	from	component	180:188	arg1	oligosaccharides					204:219	human milk oligosaccharides	193:219	human milk oligosaccharides (HMOs)	193:226	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	1	61	theme	microbiota	294:303	arg1	component					180:188	the richest component	168:188	the richest component in human milk oligosaccharides (HMOs)	168:226	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	1	61	theme	microbiota	294:303	arg1	colonization					270:281	the colonization	266:281	the colonization of harmful microbiota	266:303	As the richest component in human milk oligosaccharides (HMOs), 2'-fucosyllactose (2'-FL) can reduce the colonization of harmful microbiota in vivo, thus lowering the risk of infection; however, the mechanism for this is still unclear.					
32369957	0	62	theme	Adhesion	155:162	arg1	Regulation					95:104	the Regulation	91:104	the Regulation of Gut Microbiota	91:122	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	0	62	theme	Adhesion	155:162	arg1	Inhibition					132:141	the Inhibition	128:141	the Inhibition of Pathogen Adhesion	128:162	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	5	63	theme	Akkermansia	1198:1208	arg1	abundance					1185:1193	the abundance	1181:1193	the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria	1181:1371	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	5	64	theme	harmful	1356:1362	arg1	bacteria					1364:1371	harmful bacteria	1356:1371	harmful bacteria	1356:1371	2'-FL significantly increased the abundance of Akkermansia, a potential probiotic, which may represent the fundamental means by which 2'-FL enhances the expression of mucin and reduces the colonization of harmful bacteria.					
32369957	4	65	theme	O157	1027:1030	arg1	reduction					1057:1065	about 30% reduction	1047:1065	about 30% reduction	1047:1065	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	4	65	theme	O157	1027:1030	arg1	adhesion					1037:1044	E. coli O157 cell adhesion	1019:1044	E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	1019:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	0	66	theme	Pathogen	146:153	arg1	Adhesion					155:162	Pathogen Adhesion	146:162	Pathogen Adhesion	146:162	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	6	67	theme	infections	1461:1470	arg1	use					1408:1410	the use	1404:1410	the use of 2'-FL in the prevention of foodborne pathogen infections in human	1404:1479	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	6	68	theme	current	1378:1384	arg1	study					1386:1390	The current study	1374:1390	The current study	1374:1390	The current study may support the use of 2'-FL in the prevention of foodborne pathogen infections in human.					
32369957	2	69	theme	Escherichia	427:437	arg1	O157					444:447	Escherichia coli O157	427:447	Escherichia coli O157 infection in healthy adult mice	427:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	2	70	from	infection	449:457	arg1	mice					476:479	healthy adult mice	462:479	healthy adult mice	462:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	0	71	theme	O157	60:63	arg1	Infection					65:73	E. Coli O157 Infection	52:73	E. Coli O157 Infection	52:73	The Protective Effects of 2'-Fucosyllactose against E. Coli O157 Infection Are Mediated by the Regulation of Gut Microbiota and the Inhibition of Pathogen Adhesion.					
32369957	4	72	theme	microbiota	1127:1136	arg1	composition					1138:1148	gut microbiota composition	1123:1148	gut microbiota composition	1123:1148	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	3	73	theme	barrier	850:856	arg1	function					858:865	intestinal barrier function	839:865	intestinal barrier function	839:865	The results showed that 2'-FL intake reduced E. coli O157 colonization in mice intestine by more than 90% (p < 0.001), and it also reduced intestinal inflammation, increased the content of fecal short-chain fatty acids, and enhanced intestinal barrier function.					
32369957	2	74	from	effects	589:595	arg1	mice					600:603	mice	600:603	mice	600:603	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
32369957	4	75	theme	mucins	940:945	arg1	expression					926:935	the increased expression	912:935	the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001)	912:1077	These beneficial effects were attributed to the increased expression of mucins such as MUC2 (increased by more than 20%, p < 0.001), and inhibition of E. coli O157 cell adhesion (about 30% reduction, p < 0.001), and were associated with the modulation of gut microbiota composition.					
32369957	2	76	from	mice	476:479	arg1	model					418:422	a model	416:422	a model of Escherichia coli O157 infection in healthy adult mice	416:479	In this study, a model of Escherichia coli O157 infection in healthy adult mice was established to explore the effect of 2'-FL intervention on E. coli O157 colonization and its protective effects on mice.					
33348539	4	0	theme	χ	860:860	arg1	contribution					844:855	the contribution	840:855	the contribution of χ	840:860	Cellulose dissolution increased as a function of increasing both variables; the contribution of χ was larger than that of T in some cases.					
33348539	4	0	theme	χ	860:860	arg1	larger					866:871	larger	866:871	larger	866:871	Cellulose dissolution increased as a function of increasing both variables; the contribution of χ was larger than that of T in some cases.					
33348539	4	1	from	T	886:886	arg1	cases					896:900	some cases	891:900	some cases	891:900	Cellulose dissolution increased as a function of increasing both variables; the contribution of χ was larger than that of T in some cases.					
33348539	0	2	theme	Solvent	173:179	arg1	Composition					147:157	Composition	147:157	Composition	147:157	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	0	2	theme	Solvent	173:179	arg1	Temperature					131:141	Temperature	131:141	Temperature	131:141	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	2	3	theme	mole	644:647	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	10	4	theme	statistical	1656:1666	arg1	model					1668:1672	the statistical model	1652:1672	the statistical model	1652:1672	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	2	5	theme	cellulose	574:582	arg1	fraction					545:552	the mass fraction	536:552	the mass fraction (in %) of dissolved cellulose (MCC-m%)	536:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	8	6	theme	dissolution	1396:1406	arg1	efficiency					1408:1417	cellulose dissolution efficiency	1386:1417	cellulose dissolution efficiency	1386:1417	These results show that there is ground for using medium empirical polarity to assess cellulose dissolution efficiency.					
33348539	0	7	from	Dissolution	10:20	arg1	Mixtures					25:32	Mixtures	25:32	Mixtures of Ionic Liquids and Dimethyl Sulfoxide	25:72	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	0	8	theme	Binary	166:171	arg1	Solvent					173:179	the Binary Solvent	162:179	the Binary Solvent	162:179	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	1	9	theme	undec-7-ene-8-ium	425:441	arg1	acetate					443:449	1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate	401:449	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	8	10	theme	cellulose	1386:1394	arg1	efficiency					1408:1417	cellulose dissolution efficiency	1386:1417	cellulose dissolution efficiency	1386:1417	These results show that there is ground for using medium empirical polarity to assess cellulose dissolution efficiency.					
33348539	2	11	theme	mass	540:543	arg1	fraction					545:552	the mass fraction	536:552	the mass fraction (in %) of dissolved cellulose (MCC-m%)	536:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	1	12	theme	allylbenzyldimethylammonium	316:342	arg1	acetate					344:350	allylbenzyldimethylammonium acetate	316:350	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	2	13	theme	MCC-m	585:589	arg1	%					590:590	MCC-m%	585:590	MCC-m%	585:590	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	13	theme	MCC-m	585:589	arg1	cellulose					574:582	dissolved cellulose	564:582	dissolved cellulose (MCC-m%)	564:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	10	14	theme	approach	1700:1707	arg1	labor					1761:1765	labor	1761:1765	labor	1761:1765	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	14	theme	approach	1700:1707	arg1	material					1772:1779	material	1772:1779	material	1772:1779	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	14	theme	approach	1700:1707	arg1	time					1755:1758	saving time	1748:1758	saving time	1748:1758	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	14	theme	approach	1700:1707	arg1	usefulness					1682:1691	the usefulness	1678:1691	the usefulness of our approach to predict cellulose dissolution	1678:1740	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	14	theme	approach	1700:1707	arg1	robustness					1638:1647	the robustness	1634:1647	the robustness of the statistical model	1634:1672	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	1	15	theme	binary	248:253	arg1	mixtures					255:262	binary mixtures	248:262	binary mixtures of dimethyl sulfoxide (DMSO)	248:291	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	0	16	theme	Quantitative	77:88	arg1	Assessment					90:99	A Quantitative Assessment	75:99	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.	0:180	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	6	17	dep	liquid	1203:1208	arg1	IL					1211:1212	IL	1211:1212	IL	1211:1212	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	18	theme	2,6-dichloro-4-	1056:1070	arg1	phenolate					1103:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate	1031:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB)	1031:1116	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	18	theme	2,6-dichloro-4-	1056:1070	arg1	WB					1114:1115	WB	1114:1115	WB	1114:1115	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	9	19	theme	statistical	1510:1520	arg1	model					1522:1526	the statistical model	1506:1526	the statistical model	1506:1526	We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally.					
33348539	6	20	theme	ionic	1197:1201	arg1	mixtures					1220:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	7	21	theme	fixed	1269:1273	arg1	T					1266:1266	T	1266:1266	T (fixed χ)	1266:1276	The E(WB) correlated perfectly with T (fixed χ) and with χ (fixed T).					
33348539	7	21	theme	fixed	1269:1273	arg1	χ					1275:1275	fixed χ	1269:1275	fixed χ	1269:1275	The E(WB) correlated perfectly with T (fixed χ) and with χ (fixed T).					
33348539	6	22	theme	polarity	1147:1154	arg1	WB					1158:1159	WB	1158:1159	WB	1158:1159	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	22	theme	polarity	1147:1154	arg1	E					1156:1156	the empirical polarity E	1133:1156	the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures	1133:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	1	23	theme	tetramethylguanidinium	452:473	arg1	acetate					475:481	tetramethylguanidinium acetate	452:481	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	6	24	theme	probe	1050:1054	arg1	phenolate					1103:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate	1031:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB)	1031:1116	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	24	theme	probe	1050:1054	arg1	WB					1114:1115	WB	1114:1115	WB	1114:1115	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	2	25	theme	=	669:669	arg1	χ					667:667	χ = 0.4	667:673	χ = 0.4	667:673	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	5	26	theme	dissolution	986:996	arg1	efficiency					998:1007	the cellulose dissolution efficiency	972:1007	the cellulose dissolution efficiency of the solvent	972:1022	Solvent empirical polarity was qualitatively employed to rationalize the cellulose dissolution efficiency of the solvent.					
33348539	2	27	from	fraction	545:552	arg1	%					558:558	%	558:558	%	558:558	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	28	theme	χ	667:667	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	28	theme	χ	667:667	arg1	dependence					522:531	the dependence	518:531	the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%)	518:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	6	29	dep	cellobiose	1165:1174	arg1	model					1179:1183	a model	1177:1183	a model for MCC	1177:1191	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	5	30	theme	cellulose	976:984	arg1	efficiency					998:1007	the cellulose dissolution efficiency	972:1007	the cellulose dissolution efficiency of the solvent	972:1022	Solvent empirical polarity was qualitatively employed to rationalize the cellulose dissolution efficiency of the solvent.					
33348539	9	31	dep	those	1479:1483	arg1	determined					1532:1541	determined	1532:1541	determined the corresponding MCC-m% experimentally	1532:1581	We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally.					
33348539	9	31	dep	those	1479:1483	arg1	employed					1485:1492	employed	1485:1492	employed to generate the statistical model	1485:1526	We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally.					
33348539	3	32	from	dependence	731:740	arg1	T					755:755	T	755:755	T	755:755	We derived equations that quantified the dependence of MCC-m% on T and χ.					
33348539	3	32	from	dependence	731:740	arg1	χ					761:761	χ	761:761	χ	761:761	We derived equations that quantified the dependence of MCC-m% on T and χ.					
33348539	10	33	theme	cellulose	1720:1728	arg1	dissolution					1730:1740	cellulose dissolution	1720:1740	cellulose dissolution	1720:1740	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	0	34	theme	Cellulose	0:8	arg1	Dissolution					10:20	Cellulose Dissolution	0:20	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.	0:180	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	10	35	theme	model	1668:1672	arg1	labor					1761:1765	labor	1761:1765	labor	1761:1765	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	35	theme	model	1668:1672	arg1	material					1772:1779	material	1772:1779	material	1772:1779	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	35	theme	model	1668:1672	arg1	time					1755:1758	saving time	1748:1758	saving time	1748:1758	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	35	theme	model	1668:1672	arg1	usefulness					1682:1691	the usefulness	1678:1691	the usefulness of our approach to predict cellulose dissolution	1678:1740	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	10	35	theme	model	1668:1672	arg1	robustness					1638:1647	the robustness	1634:1647	the robustness of the statistical model	1634:1672	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	1	36	theme	dimethyl	267:274	arg1	sulfoxide					276:284	dimethyl sulfoxide	267:284	dimethyl sulfoxide (DMSO)	267:291	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	36	theme	dimethyl	267:274	arg1	DMSO					287:290	DMSO	287:290	DMSO	287:290	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	0	37	theme	Importance	117:126	arg1	Assessment					90:99	A Quantitative Assessment	75:99	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.	0:180	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	2	38	from	fraction	649:656	arg1	%					558:558	%	558:558	%	558:558	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	38	from	fraction	649:656	arg1	temperature					600:610	the temperature	596:610	the temperature	596:610	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	1	39	theme	sulfoxide	276:284	arg1	liquids					307:313	the ionic liquids	297:313	the ionic liquids	297:313	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	39	theme	sulfoxide	276:284	arg1	mixtures					255:262	binary mixtures	248:262	binary mixtures of dimethyl sulfoxide (DMSO)	248:291	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	0	40	theme	Relative	108:115	arg1	Importance					117:126	the Relative Importance	104:126	the Relative Importance of Temperature and Composition of the Binary Solvent	104:179	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	9	41	theme	%	1449:1449	arg1	values					1434:1439	values	1434:1439	values of MCC-m%	1434:1449	We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally.					
33348539	6	42	from	E	1156:1156	arg1	mixtures					1220:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	2	43	theme	0.6	676:678	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	43	theme	0.6	676:678	arg1	dependence					522:531	the dependence	518:531	the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%)	518:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	0	44	theme	Ionic	37:41	arg1	Liquids					43:49	Ionic Liquids	37:49	Ionic Liquids	37:49	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	0	45	theme	Temperature	131:141	arg1	Importance					117:126	the Relative Importance	104:126	the Relative Importance of Temperature and Composition of the Binary Solvent	104:179	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	6	46	theme	empirical	1137:1145	arg1	WB					1158:1159	WB	1158:1159	WB	1158:1159	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	46	theme	empirical	1137:1145	arg1	E					1156:1156	the empirical polarity E	1133:1156	the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures	1133:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	5	47	theme	solvent	1016:1022	arg1	efficiency					998:1007	the cellulose dissolution efficiency	972:1007	the cellulose dissolution efficiency of the solvent	972:1022	Solvent empirical polarity was qualitatively employed to rationalize the cellulose dissolution efficiency of the solvent.					
33348539	2	48	theme	fraction	545:552	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	48	theme	fraction	545:552	arg1	dependence					522:531	the dependence	518:531	the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%)	518:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	1	49	theme	-3-methylimidazolium	371:390	arg1	2-methoxyethyl					356:369	2-methoxyethyl	356:369	2-methoxyethyl	356:369	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	49	theme	-3-methylimidazolium	371:390	arg1	acetate					392:398	1-(2-methoxyethyl)-3-methylimidazolium acetate	353:398	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	10	50	theme	excellent	1588:1596	arg1	agreement					1598:1606	The excellent agreement	1584:1606	The excellent agreement between both values	1584:1626	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	1	51	from	dissolution	197:207	arg1	liquids					307:313	the ionic liquids	297:313	the ionic liquids	297:313	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	51	from	dissolution	197:207	arg1	mixtures					255:262	binary mixtures	248:262	binary mixtures of dimethyl sulfoxide (DMSO)	248:291	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	9	52	theme	corresponding	1547:1559	arg1	%					1566:1566	the corresponding MCC-m%	1543:1566	the corresponding MCC-m%	1543:1566	We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally.					
33348539	0	53	theme	Composition	147:157	arg1	Importance					117:126	the Relative Importance	104:126	the Relative Importance of Temperature and Composition of the Binary Solvent	104:179	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	6	54	theme	-DMSO	1214:1218	arg1	mixtures					1220:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	8	55	theme	empirical	1357:1365	arg1	polarity					1367:1374	empirical polarity	1357:1374	empirical polarity	1357:1374	These results show that there is ground for using medium empirical polarity to assess cellulose dissolution efficiency.					
33348539	0	56	theme	Liquids	43:49	arg1	Mixtures					25:32	Mixtures	25:32	Mixtures of Ionic Liquids and Dimethyl Sulfoxide	25:72	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	4	57	theme	Cellulose	764:772	arg1	dissolution					774:784	Cellulose dissolution	764:784	Cellulose dissolution	764:784	Cellulose dissolution increased as a function of increasing both variables; the contribution of χ was larger than that of T in some cases.					
33348539	2	58	theme	dissolved	564:572	arg1	%					590:590	MCC-m%	585:590	MCC-m%	585:590	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	58	theme	dissolved	564:572	arg1	cellulose					574:582	dissolved cellulose	564:582	dissolved cellulose (MCC-m%)	564:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	7	59	theme	fixed	1290:1294	arg1	χ					1287:1287	χ	1287:1287	χ (fixed T)	1287:1297	The E(WB) correlated perfectly with T (fixed χ) and with χ (fixed T).					
33348539	7	59	theme	fixed	1290:1294	arg1	T					1296:1296	fixed T	1290:1296	fixed T	1290:1296	The E(WB) correlated perfectly with T (fixed χ) and with χ (fixed T).					
33348539	2	60	from	dependence	522:531	arg1	%					558:558	%	558:558	%	558:558	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	60	from	dependence	522:531	arg1	temperature					600:610	the temperature	596:610	the temperature	596:610	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	9	61	theme	other	1468:1472	arg1	conditions					1457:1466	conditions	1457:1466	conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally	1457:1581	We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally.					
33348539	6	62	theme	cellobiose	1165:1174	arg1	WB					1158:1159	WB	1158:1159	WB	1158:1159	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	62	theme	cellobiose	1165:1174	arg1	E					1156:1156	the empirical polarity E	1133:1156	the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures	1133:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	1	63	theme	1,8-diazabicyclo	401:416	arg1	acetate					443:449	1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate	401:449	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	64	dep	dissolution	197:207	arg1	2-methoxyethyl					356:369	2-methoxyethyl	356:369	2-methoxyethyl	356:369	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	64	dep	dissolution	197:207	arg1	acetate					392:398	1-(2-methoxyethyl)-3-methylimidazolium acetate	353:398	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	64	dep	dissolution	197:207	arg1	acetate					475:481	tetramethylguanidinium acetate	452:481	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	64	dep	dissolution	197:207	arg1	acetate					443:449	1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate	401:449	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	64	dep	dissolution	197:207	arg1	acetate					344:350	allylbenzyldimethylammonium acetate	316:350	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	3	65	theme	%	750:750	arg1	dependence					731:740	the dependence	727:740	the dependence of MCC-m% on T and χ	727:761	We derived equations that quantified the dependence of MCC-m% on T and χ.					
33348539	1	66	theme	microcrystalline	212:227	arg1	MCC					240:242	MCC	240:242	MCC	240:242	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	1	66	theme	microcrystalline	212:227	arg1	cellulose					229:237	microcrystalline cellulose	212:237	microcrystalline cellulose (MCC)	212:243	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	6	67	theme	solvatochromic	1035:1048	arg1	phenolate					1103:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate	1031:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB)	1031:1116	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	67	theme	solvatochromic	1035:1048	arg1	WB					1114:1115	WB	1114:1115	WB	1114:1115	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	5	68	theme	Solvent	903:909	arg1	polarity					921:928	Solvent empirical polarity	903:928	Solvent empirical polarity	903:928	Solvent empirical polarity was qualitatively employed to rationalize the cellulose dissolution efficiency of the solvent.					
33348539	2	69	theme	DMSO	661:664	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	69	theme	DMSO	661:664	arg1	dependence					522:531	the dependence	518:531	the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%)	518:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	1	70	theme	cellulose	229:237	arg1	dissolution					197:207	the dissolution	193:207	the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate	193:481	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	0	71	theme	Sulfoxide	64:72	arg1	Mixtures					25:32	Mixtures	25:32	Mixtures of Ionic Liquids and Dimethyl Sulfoxide	25:72	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	5	72	theme	empirical	911:919	arg1	polarity					921:928	Solvent empirical polarity	903:928	Solvent empirical polarity	903:928	Solvent empirical polarity was qualitatively employed to rationalize the cellulose dissolution efficiency of the solvent.					
33348539	0	73	dep	Dissolution	10:20	arg1	Assessment					90:99	A Quantitative Assessment	75:99	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.	0:180	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	2	74	theme	0.8	685:687	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	74	theme	0.8	685:687	arg1	dependence					522:531	the dependence	518:531	the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%)	518:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	75	from	%	558:558	arg1	fraction					649:656	the mole fraction	640:656	the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8	640:687	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	2	75	from	%	558:558	arg1	dependence					522:531	the dependence	518:531	the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%)	518:591	Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χ = 0.4, 0.6, and 0.8.					
33348539	0	76	theme	Dimethyl	55:62	arg1	Sulfoxide					64:72	Dimethyl Sulfoxide	55:72	Dimethyl Sulfoxide	55:72	Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent.					
33348539	1	77	theme	ionic	301:305	arg1	liquids					307:313	the ionic liquids	297:313	the ionic liquids	297:313	We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate.					
33348539	6	78	theme	2,4,6-triphenylpyridinium-1-yl	1072:1101	arg1	phenolate					1103:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate	1031:1111	the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB)	1031:1116	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	6	78	theme	2,4,6-triphenylpyridinium-1-yl	1072:1101	arg1	WB					1114:1115	WB	1114:1115	WB	1114:1115	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
33348539	10	79	theme	saving	1748:1753	arg1	time					1755:1758	saving time	1748:1758	saving time	1748:1758	The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.					
33348539	6	80	theme	liquid	1203:1208	arg1	mixtures					1220:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	ionic liquid (IL)-DMSO mixtures	1197:1227	Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity E(WB) of cellobiose (a model for MCC) in ionic liquid (IL)-DMSO mixtures.					
34863972	4	0	theme	Fc	739:740	arg1	affinity					751:758	Fc receptor affinity	739:758	Fc receptor affinity	739:758	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	2	1	theme	charge	337:342	arg1	variants					344:351	size and charge variants	328:351	size and charge variants	328:351	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	0	2	theme	Adalimumab	74:83	arg1	Biosimilar					60:69	a Biosimilar	58:69	a Biosimilar of Adalimumab (Humira)	58:92	Physicochemical and Functional Characterization of HS016, a Biosimilar of Adalimumab (Humira).					
34863972	0	2	theme	Adalimumab	74:83	arg1	Physicochemical					0:14	Physicochemical	0:14	Physicochemical	0:14	Physicochemical and Functional Characterization of HS016, a Biosimilar of Adalimumab (Humira).					
34863972	5	3	theme	biological	931:940	arg1	activity					942:949	biological activity	931:949	biological activity	931:949	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	1	4	theme	physical	235:242	arg1	chemistry					244:252	physical chemistry	235:252	physical chemistry	235:252	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	5	5	theme	adalimumab	964:973	arg1	similar					980:986	similar	980:986	similar	980:986	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	5	theme	adalimumab	964:973	arg1	structures					887:896	The primary and higher order structures	858:896	The primary and higher order structures	858:896	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	5	theme	adalimumab	964:973	arg1	properties					915:924	physicochemical properties	899:924	physicochemical properties	899:924	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	5	theme	adalimumab	964:973	arg1	activity					942:949	biological activity	931:949	biological activity	931:949	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	4	6	theme	receptor	742:749	arg1	affinity					751:758	Fc receptor affinity	739:758	Fc receptor affinity	739:758	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	2	7	theme	order	310:314	arg1	structures					316:325	The primary and higher order structures	287:325	The primary and higher order structures	287:325	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	4	8	theme	binding	721:727	arg1	activity					729:736	antigen binding activity	713:736	antigen binding activity	713:736	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	4	9	theme	antibody-dependent	761:778	arg1	cytotoxicity					794:805	antibody-dependent cell-mediated cytotoxicity	761:805	antibody-dependent cell-mediated cytotoxicity	761:805	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	5	10	theme	HS016	954:958	arg1	similar					980:986	similar	980:986	similar	980:986	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	10	theme	HS016	954:958	arg1	structures					887:896	The primary and higher order structures	858:896	The primary and higher order structures	858:896	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	10	theme	HS016	954:958	arg1	properties					915:924	physicochemical properties	899:924	physicochemical properties	899:924	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	10	theme	HS016	954:958	arg1	activity					942:949	biological activity	931:949	biological activity	931:949	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	1	11	theme	reference	148:156	arg1	adalimumab					166:175	the reference product adalimumab	144:175	the reference product adalimumab (Humira)	144:184	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	1	11	theme	reference	148:156	arg1	Humira					178:183	Humira	178:183	Humira	178:183	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	4	12	theme	complemented-dependent	810:831	arg1	cytotoxicity					833:844	complemented-dependent cytotoxicity	810:844	complemented-dependent cytotoxicity	810:844	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	3	13	theme	relative	520:527	arg1	levels					529:534	the relative levels	516:534	the relative levels of methionine oxidation	516:558	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	1	14	theme	product	158:164	arg1	adalimumab					166:175	the reference product adalimumab	144:175	the reference product adalimumab (Humira)	144:184	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	1	14	theme	product	158:164	arg1	Humira					178:183	Humira	178:183	Humira	178:183	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	2	15	theme	stability	461:469	arg1	samples					471:477	unstressed and stability samples	446:477	unstressed and stability samples	446:477	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	16	gly	glycosylation	354:366	arg1	antibodies					407:416	both antibodies	402:416	both antibodies	402:416	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	17	theme	higher	303:308	arg1	structures					316:325	The primary and higher order structures	287:325	The primary and higher order structures	287:325	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	1	18	theme	adalimumab	166:175	arg1	characterization					99:114	The characterization	95:114	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities	95:211	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	1	19	theme	functional	258:267	arg1	tests					280:284	functional similarity tests	258:284	functional similarity tests	258:284	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	5	20	theme	order	881:885	arg1	similar					980:986	similar	980:986	similar	980:986	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	20	theme	order	881:885	arg1	structures					887:896	The primary and higher order structures	858:896	The primary and higher order structures	858:896	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	3	21	theme	low	561:563	arg1	components					582:591	low molecular weight components	561:591	low molecular weight components	561:591	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	2	22	theme	glycosylation	354:366	arg1	profiles					368:375	glycosylation profiles	354:375	glycosylation profiles	354:375	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	23	theme	antibodies	407:416	arg1	potency					391:397	in vitro potency	382:397	in vitro potency	382:397	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	23	theme	antibodies	407:416	arg1	variants					344:351	size and charge variants	328:351	size and charge variants	328:351	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	23	theme	antibodies	407:416	arg1	structures					316:325	The primary and higher order structures	287:325	The primary and higher order structures	287:325	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	23	theme	antibodies	407:416	arg1	profiles					368:375	glycosylation profiles	354:375	glycosylation profiles	354:375	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	1	24	theme	similarity	269:278	arg1	tests					280:284	functional similarity tests	258:284	functional similarity tests	258:284	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	2	25	theme	primary	291:297	arg1	structures					316:325	The primary and higher order structures	287:325	The primary and higher order structures	287:325	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	0	26	theme	Functional	20:29	arg1	Characterization					31:46	Functional Characterization	20:46	Functional Characterization	20:46	Physicochemical and Functional Characterization of HS016, a Biosimilar of Adalimumab (Humira).					
34863972	3	27	theme	oxidation	550:558	arg1	levels					529:534	the relative levels	516:534	the relative levels of methionine oxidation	516:558	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	27	theme	oxidation	550:558	arg1	components					582:591	low molecular weight components	561:591	low molecular weight components	561:591	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	27	theme	oxidation	550:558	arg1	variant					610:616	terminal lysine variant	594:616	terminal lysine variant	594:616	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	27	theme	oxidation	550:558	arg1	mannoses					624:631	high mannoses	619:631	high mannoses	619:631	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	27	theme	oxidation	550:558	arg1	glycans					652:658	galactosylated glycans	637:658	galactosylated glycans	637:658	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	5	28	theme	primary	862:868	arg1	similar					980:986	similar	980:986	similar	980:986	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	28	theme	primary	862:868	arg1	structures					887:896	The primary and higher order structures	858:896	The primary and higher order structures	858:896	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	0	29	theme	HS016	51:55	arg1	Physicochemical					0:14	Physicochemical	0:14	Physicochemical	0:14	Physicochemical and Functional Characterization of HS016, a Biosimilar of Adalimumab (Humira).					
34863972	0	29	theme	HS016	51:55	arg1	Characterization					31:46	Functional Characterization	20:46	Functional Characterization	20:46	Physicochemical and Functional Characterization of HS016, a Biosimilar of Adalimumab (Humira).					
34863972	0	29	theme	HS016	51:55	arg1	Biosimilar					60:69	a Biosimilar	58:69	a Biosimilar of Adalimumab (Humira)	58:92	Physicochemical and Functional Characterization of HS016, a Biosimilar of Adalimumab (Humira).					
34863972	3	30	theme	galactosylated	637:650	arg1	glycans					652:658	galactosylated glycans	637:658	galactosylated glycans	637:658	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	5	31	theme	physicochemical	899:913	arg1	properties					915:924	physicochemical properties	899:924	physicochemical properties	899:924	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	2	32	theme	unstressed	446:455	arg1	samples					471:477	unstressed and stability samples	446:477	unstressed and stability samples	446:477	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	3	33	theme	high	619:622	arg1	mannoses					624:631	high mannoses	619:631	high mannoses	619:631	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	2	34	dep	in	382:383	arg1	vitro					385:389	vitro	385:389	vitro	385:389	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	35	theme	in	382:383	arg1	potency					391:397	in vitro potency	382:397	in vitro potency	382:397	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	2	36	dep	samples	471:477	arg1	both					437:440	both	437:440	both	437:440	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	3	37	theme	molecular	565:573	arg1	components					582:591	low molecular weight components	561:591	low molecular weight components	561:591	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	38	located	observed	504:511	arg1	components					582:591	low molecular weight components	561:591	low molecular weight components	561:591	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	38	located	observed	504:511	arg1	mannoses					624:631	high mannoses	619:631	high mannoses	619:631	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	38	located	observed	504:511	arg2	differences					487:497	Slight differences	480:497	Slight differences	480:497	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	38	located	observed	504:511	arg1	variant					610:616	terminal lysine variant	594:616	terminal lysine variant	594:616	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	38	located	observed	504:511	arg1	glycans					652:658	galactosylated glycans	637:658	galactosylated glycans	637:658	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	38	located	observed	504:511	arg1	levels					529:534	the relative levels	516:534	the relative levels of methionine oxidation	516:558	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	2	39	theme	size	328:331	arg1	variants					344:351	size and charge variants	328:351	size and charge variants	328:351	The primary and higher order structures, size and charge variants, glycosylation profiles, and in vitro potency of both antibodies were characterized both for unstressed and stability samples.					
34863972	3	40	theme	weight	575:580	arg1	components					582:591	low molecular weight components	561:591	low molecular weight components	561:591	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	3	41	theme	Slight	480:485	arg1	differences					487:497	Slight differences	480:497	Slight differences	480:497	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	4	42	theme	antigen	713:719	arg1	activity					729:736	antigen binding activity	713:736	antigen binding activity	713:736	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	3	43	theme	methionine	539:548	arg1	oxidation					550:558	methionine oxidation	539:558	methionine oxidation	539:558	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	4	44	theme	cell-mediated	780:792	arg1	cytotoxicity					794:805	antibody-dependent cell-mediated cytotoxicity	761:805	antibody-dependent cell-mediated cytotoxicity	761:805	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	1	45	theme	biosimilar	121:130	arg1	HS016					137:141	a biosimilar drug HS016	119:141	a biosimilar drug HS016	119:141	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	4	46	from	differences	698:708	arg1	affinity					751:758	Fc receptor affinity	739:758	Fc receptor affinity	739:758	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	4	46	from	differences	698:708	arg1	cytotoxicity					794:805	antibody-dependent cell-mediated cytotoxicity	761:805	antibody-dependent cell-mediated cytotoxicity	761:805	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	4	46	from	differences	698:708	arg1	cytotoxicity					833:844	complemented-dependent cytotoxicity	810:844	complemented-dependent cytotoxicity	810:844	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	4	46	from	differences	698:708	arg1	activity					729:736	antigen binding activity	713:736	antigen binding activity	713:736	However, no differences in antigen binding activity, Fc receptor affinity, antibody-dependent cell-mediated cytotoxicity or complemented-dependent cytotoxicity were found.					
34863972	1	47	theme	biosimilarities	197:211	arg1	characterization					99:114	The characterization	95:114	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities	95:211	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	5	48	theme	higher	874:879	arg1	similar					980:986	similar	980:986	similar	980:986	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	5	48	theme	higher	874:879	arg1	structures					887:896	The primary and higher order structures	858:896	The primary and higher order structures	858:896	The primary and higher order structures, physicochemical properties, and biological activity of HS016 and adalimumab were similar.					
34863972	3	49	theme	terminal	594:601	arg1	variant					610:616	terminal lysine variant	594:616	terminal lysine variant	594:616	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	1	50	theme	drug	132:135	arg1	HS016					137:141	a biosimilar drug HS016	119:141	a biosimilar drug HS016	119:141	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
34863972	3	51	theme	lysine	603:608	arg1	variant					610:616	terminal lysine variant	594:616	terminal lysine variant	594:616	Slight differences were observed in the relative levels of methionine oxidation, low molecular weight components, terminal lysine variant, high mannoses and galactosylated glycans between HS016 and Humira.					
34863972	1	52	theme	HS016	137:141	arg1	characterization					99:114	The characterization	95:114	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities	95:211	The characterization of a biosimilar drug HS016, the reference product adalimumab (Humira), and their biosimilarities were determined using physical chemistry and functional similarity tests.					
32100612	0	0	theme	bone	111:114	arg1	regeneration					116:127	bone regeneration	111:127	bone regeneration	111:127	Cellulose nanocrystals reinforced gelatin/bioactive glass nanocomposite scaffolds for potential application in bone regeneration.					
32100612	1	1	theme	physico-mechanical	200:217	arg1	properties					219:228	physico-mechanical properties	200:228	physico-mechanical properties of natural bone	200:244	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	1	2	theme	bone	298:301	arg1	regeneration					310:321	bone tissue regeneration	298:321	bone tissue regeneration	298:321	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	4	3	theme	freeze-drying	777:789	arg1	technique					791:799	freeze-drying technique	777:799	freeze-drying technique	777:799	The porous composite BG-Gel-CNC was simultaneously constructed by in-situ composite method and freeze-drying technique.					
32100612	1	4	theme	tissue	303:308	arg1	regeneration					310:321	bone tissue regeneration	298:321	bone tissue regeneration	298:321	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	7	5	theme	bone	1233:1236	arg1	regeneration					1245:1256	bone tissue regeneration	1233:1256	bone tissue regeneration	1233:1256	These findings therefore suggested their potential application to function as effective scaffold materials in bone tissue regeneration.					
32100612	1	6	theme	regeneration	310:321	arg1	development					283:293	the development	279:293	the development of bone tissue regeneration	279:321	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	7	7	theme	tissue	1238:1243	arg1	regeneration					1245:1256	bone tissue regeneration	1233:1256	bone tissue regeneration	1233:1256	These findings therefore suggested their potential application to function as effective scaffold materials in bone tissue regeneration.					
32100612	3	8	theme	mechanical	561:570	arg1	properties					572:581	favorable biocompatibility and impressive mechanical properties	519:581	favorable biocompatibility and impressive mechanical properties	519:581	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	1	9	theme	three-dimensional	168:184	arg1	structure					186:194	precise porous three-dimensional structure	153:194	precise porous three-dimensional structure	153:194	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	5	10	theme	wettability	938:948	arg1	conducive					960:968	conducive	960:968	conducive	960:968	The results manifested that the scaffolds incorporated with CNC showed a desirable compressive strength compared to the control, better wettability, which is conducive to better adhesion, growth and proliferation of cells.					
32100612	5	10	theme	wettability	938:948	arg1	control					922:928	the control	918:928	the control	918:928	The results manifested that the scaffolds incorporated with CNC showed a desirable compressive strength compared to the control, better wettability, which is conducive to better adhesion, growth and proliferation of cells.					
32100612	3	11	theme	biocompatibility	529:544	arg1	properties					572:581	favorable biocompatibility and impressive mechanical properties	519:581	favorable biocompatibility and impressive mechanical properties	519:581	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	2	12	theme	serious	433:439	arg1	attention					441:449	serious attention	433:449	serious attention in its fabrication	433:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	5	13	theme	desirable	875:883	arg1	strength					897:904	a desirable compressive strength	873:904	a desirable compressive strength	873:904	The results manifested that the scaffolds incorporated with CNC showed a desirable compressive strength compared to the control, better wettability, which is conducive to better adhesion, growth and proliferation of cells.					
32100612	2	14	theme	attention	441:449	arg1	strength					366:373	mechanical strength	355:373	mechanical strength of scaffold materials for bone regeneration	355:417	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	2	14	theme	attention	441:449	arg1	subject					422:428	a subject	420:428	a subject of serious attention in its fabrication	420:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	7	15	theme	effective	1201:1209	arg1	materials					1220:1228	effective scaffold materials	1201:1228	effective scaffold materials in bone tissue regeneration	1201:1256	These findings therefore suggested their potential application to function as effective scaffold materials in bone tissue regeneration.					
32100612	7	16	theme	potential	1164:1172	arg1	application					1174:1184	their potential application to function as effective scaffold materials in bone tissue regeneration	1158:1256	their potential application to function as effective scaffold materials in bone tissue regeneration	1158:1256	These findings therefore suggested their potential application to function as effective scaffold materials in bone tissue regeneration.					
32100612	1	17	theme	bone	241:244	arg1	structure					186:194	precise porous three-dimensional structure	153:194	precise porous three-dimensional structure	153:194	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	1	17	theme	bone	241:244	arg1	properties					219:228	physico-mechanical properties	200:228	physico-mechanical properties of natural bone	200:244	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	0	18	theme	Cellulose	0:8	arg1	nanocrystals					10:21	Cellulose nanocrystals	0:21	Cellulose nanocrystals reinforced	0:32	Cellulose nanocrystals reinforced gelatin/bioactive glass nanocomposite scaffolds for potential application in bone regeneration.					
32100612	7	19	theme	scaffold	1211:1218	arg1	materials					1220:1228	effective scaffold materials	1201:1228	effective scaffold materials in bone tissue regeneration	1201:1256	These findings therefore suggested their potential application to function as effective scaffold materials in bone tissue regeneration.					
32100612	2	20	from	subject	422:428	arg1	fabrication					458:468	its fabrication	454:468	its fabrication	454:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	3	21	theme	gelatin/bioactive	641:657	arg1	system					674:679	gelatin/bioactive glass (BG-Gel) system	641:679	gelatin/bioactive glass (BG-Gel) system	641:679	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	3	22	theme	cellulose	478:486	arg1	nanocrystals					488:499	cellulose nanocrystals	478:499	cellulose nanocrystals (CNC)	478:505	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	3	22	theme	cellulose	478:486	arg1	CNC					502:504	CNC	502:504	CNC	502:504	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	6	23	theme	pore	1060:1063	arg1	connectivity					1065:1076	pore connectivity	1060:1076	pore connectivity	1060:1076	In addition, appropriate porosity, pore connectivity and biocompatibility were also demonstrated.					
32100612	2	24	theme	mechanical	355:364	arg1	strength					366:373	mechanical strength	355:373	mechanical strength of scaffold materials for bone regeneration	355:417	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	2	24	theme	mechanical	355:364	arg1	subject					422:428	a subject	420:428	a subject of serious attention in its fabrication	420:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	7	25	from	materials	1220:1228	arg1	regeneration					1245:1256	bone tissue regeneration	1233:1256	bone tissue regeneration	1233:1256	These findings therefore suggested their potential application to function as effective scaffold materials in bone tissue regeneration.					
32100612	4	26	theme	composite	693:701	arg1	BG-Gel-CNC					703:712	The porous composite BG-Gel-CNC	682:712	The porous composite BG-Gel-CNC	682:712	The porous composite BG-Gel-CNC was simultaneously constructed by in-situ composite method and freeze-drying technique.					
32100612	0	27	theme	nanocomposite	58:70	arg1	scaffolds					72:80	glass nanocomposite scaffolds	52:80	glass nanocomposite scaffolds	52:80	Cellulose nanocrystals reinforced gelatin/bioactive glass nanocomposite scaffolds for potential application in bone regeneration.					
32100612	3	28	theme	impressive	550:559	arg1	properties					572:581	favorable biocompatibility and impressive mechanical properties	519:581	favorable biocompatibility and impressive mechanical properties	519:581	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	3	29	theme	BG-Gel	666:671	arg1	system					674:679	gelatin/bioactive glass (BG-Gel) system	641:679	gelatin/bioactive glass (BG-Gel) system	641:679	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	4	30	theme	in-situ	748:754	arg1	method					766:771	in-situ composite method	748:771	in-situ composite method	748:771	The porous composite BG-Gel-CNC was simultaneously constructed by in-situ composite method and freeze-drying technique.					
32100612	1	31	theme	vital	260:264	arg1	direction					266:274	a vital direction	258:274	a vital direction in the development of bone tissue regeneration	258:321	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	0	32	theme	glass	52:56	arg1	scaffolds					72:80	glass nanocomposite scaffolds	52:80	glass nanocomposite scaffolds	52:80	Cellulose nanocrystals reinforced gelatin/bioactive glass nanocomposite scaffolds for potential application in bone regeneration.					
32100612	6	33	theme	appropriate	1038:1048	arg1	porosity					1050:1057	appropriate porosity	1038:1057	appropriate porosity	1038:1057	In addition, appropriate porosity, pore connectivity and biocompatibility were also demonstrated.					
32100612	5	34	theme	compressive	885:895	arg1	strength					897:904	a desirable compressive strength	873:904	a desirable compressive strength	873:904	The results manifested that the scaffolds incorporated with CNC showed a desirable compressive strength compared to the control, better wettability, which is conducive to better adhesion, growth and proliferation of cells.					
32100612	0	35	from	application	96:106	arg1	regeneration					116:127	bone regeneration	111:127	bone regeneration	111:127	Cellulose nanocrystals reinforced gelatin/bioactive glass nanocomposite scaffolds for potential application in bone regeneration.					
32100612	5	36	dep	better	973:978	arg1	adhesion					980:987	adhesion	980:987	adhesion	980:987	The results manifested that the scaffolds incorporated with CNC showed a desirable compressive strength compared to the control, better wettability, which is conducive to better adhesion, growth and proliferation of cells.					
32100612	3	37	contain	possessing	508:517	arg1	nanocrystals					488:499	cellulose nanocrystals	478:499	cellulose nanocrystals (CNC)	478:505	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	3	37	contain	possessing	508:517	arg2	properties					572:581	favorable biocompatibility and impressive mechanical properties	519:581	favorable biocompatibility and impressive mechanical properties	519:581	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	3	37	contain	possessing	508:517	arg1	CNC					502:504	CNC	502:504	CNC	502:504	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	4	38	theme	composite	756:764	arg1	method					766:771	in-situ composite method	748:771	in-situ composite method	748:771	The porous composite BG-Gel-CNC was simultaneously constructed by in-situ composite method and freeze-drying technique.					
32100612	3	39	theme	glass	659:663	arg1	system					674:679	gelatin/bioactive glass (BG-Gel) system	641:679	gelatin/bioactive glass (BG-Gel) system	641:679	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	1	40	from	direction	266:274	arg1	development					283:293	the development	279:293	the development of bone tissue regeneration	279:321	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	2	41	theme	bone	401:404	arg1	regeneration					406:417	bone regeneration	401:417	bone regeneration	401:417	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	3	42	theme	favorable	519:527	arg1	properties					572:581	favorable biocompatibility and impressive mechanical properties	519:581	favorable biocompatibility and impressive mechanical properties	519:581	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	1	43	theme	precise	153:159	arg1	structure					186:194	precise porous three-dimensional structure	153:194	precise porous three-dimensional structure	153:194	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	2	44	from	attention	441:449	arg1	fabrication					458:468	its fabrication	454:468	its fabrication	454:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	1	45	theme	porous	161:166	arg1	structure					186:194	precise porous three-dimensional structure	153:194	precise porous three-dimensional structure	153:194	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	3	46	theme	nanocomposite	614:626	arg1	scaffolds					628:636	the nanocomposite scaffolds	610:636	the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system	610:679	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	2	47	from	fabrication	458:468	arg1	strength					366:373	mechanical strength	355:373	mechanical strength of scaffold materials for bone regeneration	355:417	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	2	47	from	fabrication	458:468	arg1	subject					422:428	a subject	420:428	a subject of serious attention in its fabrication	420:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	2	48	theme	scaffold	378:385	arg1	materials					387:395	scaffold materials	378:395	scaffold materials for bone regeneration	378:417	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	2	49	theme	materials	387:395	arg1	strength					366:373	mechanical strength	355:373	mechanical strength of scaffold materials for bone regeneration	355:417	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	2	49	theme	materials	387:395	arg1	subject					422:428	a subject	420:428	a subject of serious attention in its fabrication	420:468	This work focused on enhancing mechanical strength of scaffold materials for bone regeneration, a subject of serious attention in its fabrication.					
32100612	3	50	theme	system	674:679	arg1	scaffolds					628:636	the nanocomposite scaffolds	610:636	the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system	610:679	Hence, cellulose nanocrystals (CNC), possessing favorable biocompatibility and impressive mechanical properties, was selected to reinforce the nanocomposite scaffolds of gelatin/bioactive glass (BG-Gel) system.					
32100612	1	51	theme	natural	233:239	arg1	bone					241:244	natural bone	233:244	natural bone	233:244	Simulating components, precise porous three-dimensional structure and physico-mechanical properties of natural bone have become a vital direction in the development of bone tissue regeneration.					
32100612	4	52	theme	porous	686:691	arg1	BG-Gel-CNC					703:712	The porous composite BG-Gel-CNC	682:712	The porous composite BG-Gel-CNC	682:712	The porous composite BG-Gel-CNC was simultaneously constructed by in-situ composite method and freeze-drying technique.					
32100612	0	53	theme	potential	86:94	arg1	application					96:106	potential application	86:106	potential application in bone regeneration	86:127	Cellulose nanocrystals reinforced gelatin/bioactive glass nanocomposite scaffolds for potential application in bone regeneration.					
34489976	8	0	theme	signaling	1619:1627	arg1	pathways					1629:1636	cytokine signaling pathways	1610:1636	cytokine signaling pathways	1610:1636	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	8	1	theme	promoter	1561:1568	arg1	regions					1570:1576	promoter regions	1561:1576	promoter regions that regulate genes involved in cytokine signaling pathways	1561:1636	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	5	2	dep	control	940:946	arg1	macaques					963:970	female macaques	956:970	female macaques	956:970	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	1	3	theme	viral	240:244	arg1	infection					246:254	bacterial and viral infection	226:254	bacterial and viral infection	226:254	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	0	4	theme	Non-Human	76:84	arg1	Primates					86:93	Non-Human Primates	76:93	Non-Human Primates	76:93	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	8	5	theme	higher	1537:1542	arg1	accessibility					1544:1556	higher accessibility	1537:1556	higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways	1537:1636	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	8	6	theme	subsets	1434:1440	arg1	presence					1413:1420	The increased presence	1399:1420	The increased presence of monocyte subsets skewed towards inflammatory phenotypes	1399:1479	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	5	7	theme	CHD	914:916	arg1	effects					903:909	the effects	899:909	the effects of CHD on blood monocytes	899:935	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	8	8	theme	inflammatory	1457:1468	arg1	phenotypes					1470:1479	inflammatory phenotypes	1457:1479	inflammatory phenotypes	1457:1479	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	0	9	theme	Chronic	105:111	arg1	Drinking					121:128	Chronic Alcohol Drinking	105:128	Chronic Alcohol Drinking	105:128	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	4	10	theme	self-administration	785:803	arg1	model					758:762	a translational rhesus macaque model	727:762	a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity	727:869	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	4	11	used	utilized	718:725	arg2	we					715:716	we	715:716	we	715:716	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	5	12	theme	female	956:961	arg1	macaques					963:970	female macaques	956:970	female macaques	956:970	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	4	13	theme	translational	729:741	arg1	model					758:762	a translational rhesus macaque model	727:762	a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity	727:869	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	7	14	theme	monocyte	1258:1265	arg1	subsets					1267:1273	classical monocyte subsets	1248:1273	classical monocyte subsets	1248:1273	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	7	15	theme	classical	1248:1256	arg1	subsets					1267:1273	classical monocyte subsets	1248:1273	classical monocyte subsets	1248:1273	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	9	16	theme	hyper-inflammatory	1793:1810	arg1	response					1812:1819	a more hyper-inflammatory response	1786:1819	a more hyper-inflammatory response to LPS	1786:1826	Collectively, data presented in this manuscript demonstrate that CHD shifts classical monocyte subset composition and primes the monocytes towards a more hyper-inflammatory response to LPS, but compromised pathogen response.					
34489976	9	17	theme	pathogen	1845:1852	arg1	response					1854:1861	compromised pathogen response	1833:1861	compromised pathogen response	1833:1861	Collectively, data presented in this manuscript demonstrate that CHD shifts classical monocyte subset composition and primes the monocytes towards a more hyper-inflammatory response to LPS, but compromised pathogen response.					
34489976	8	18	theme	increased	1403:1411	arg1	presence					1413:1420	The increased presence	1399:1420	The increased presence of monocyte subsets skewed towards inflammatory phenotypes	1399:1479	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	9	19	theme	classical	1715:1723	arg1	composition					1741:1751	classical monocyte subset composition	1715:1751	classical monocyte subset composition	1715:1751	Collectively, data presented in this manuscript demonstrate that CHD shifts classical monocyte subset composition and primes the monocytes towards a more hyper-inflammatory response to LPS, but compromised pathogen response.					
34489976	5	20	from	effects	903:909	arg1	monocytes					927:935	blood monocytes	921:935	blood monocytes	921:935	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	4	21	theme	ethanol	777:783	arg1	self-administration					785:803	voluntary ethanol self-administration	767:803	voluntary ethanol self-administration	767:803	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	1	22	theme	alcohol	163:169	arg1	CHD					155:157	CHD	155:157	CHD	155:157	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	22	theme	alcohol	163:169	arg1	drinking					145:152	Chronic heavy drinking	131:152	Chronic heavy drinking (CHD) of alcohol	131:169	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	22	theme	alcohol	163:169	arg1	factor					187:192	a known risk factor	174:192	a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing	174:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	0	23	theme	Alcohol	113:119	arg1	Drinking					121:128	Chronic Alcohol Drinking	105:128	Chronic Alcohol Drinking	105:128	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	8	24	theme	regions	1570:1576	arg1	accessibility					1544:1556	higher accessibility	1537:1556	higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways	1537:1636	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	9	25	theme	monocyte	1725:1732	arg1	composition					1741:1751	classical monocyte subset composition	1715:1751	classical monocyte subset composition	1715:1751	Collectively, data presented in this manuscript demonstrate that CHD shifts classical monocyte subset composition and primes the monocytes towards a more hyper-inflammatory response to LPS, but compromised pathogen response.					
34489976	3	26	dep	ability	497:503	arg1	study					508:512	study	508:512	to study CHD	505:516	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	6	27	theme	ex	1102:1103	arg1	stimulation					1114:1124	ex vivo LPS stimulation	1102:1124	ex vivo LPS stimulation	1102:1124	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	7	28	theme	inflammation	1344:1355	arg1	markers					1313:1319	markers	1313:1319	markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway)	1313:1380	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	5	29	theme	daily	991:995	arg1	consumption					1005:1015	daily ethanol consumption	991:1015	daily ethanol consumption	991:1015	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	7	30	theme	significant	1226:1236	arg1	shifts					1238:1243	significant shifts	1226:1243	significant shifts	1226:1243	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	9	31	theme	subset	1734:1739	arg1	composition					1741:1751	classical monocyte subset composition	1715:1751	classical monocyte subset composition	1715:1751	Collectively, data presented in this manuscript demonstrate that CHD shifts classical monocyte subset composition and primes the monocytes towards a more hyper-inflammatory response to LPS, but compromised pathogen response.					
34489976	3	32	theme	drinking	559:566	arg1	patterns					568:575	human drinking patterns	553:575	human drinking patterns	553:575	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	6	33	theme	hyper-inflammatory	1071:1088	arg1	response					1090:1097	a hyper-inflammatory response	1069:1097	a hyper-inflammatory response to ex vivo LPS stimulation	1069:1124	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	0	34	theme	Transcriptional	0:14	arg1	Reprogramming					44:56	Transcriptional, Epigenetic, and Functional Reprogramming	0:56	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates	0:93	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	8	35	theme	epigenetic	1501:1510	arg1	analysis					1512:1519	epigenetic analysis	1501:1519	epigenetic analysis	1501:1519	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	3	36	theme	patterns	568:575	arg1	comorbidities					601:613	comorbidities	601:613	comorbidities	601:613	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	3	36	theme	patterns	568:575	arg1	complexities					537:548	the complexities	533:548	the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders	533:682	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	3	36	theme	patterns	568:575	arg1	factors					636:642	confounding risk factors	619:642	confounding risk factors	619:642	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	1	37	theme	known	176:180	arg1	factor					187:192	a known risk factor	174:192	a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing	174:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	37	theme	known	176:180	arg1	drinking					145:152	Chronic heavy drinking	131:152	Chronic heavy drinking (CHD) of alcohol	131:169	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	9	38	theme	compromised	1833:1843	arg1	response					1854:1861	compromised pathogen response	1833:1861	compromised pathogen response	1833:1861	Collectively, data presented in this manuscript demonstrate that CHD shifts classical monocyte subset composition and primes the monocytes towards a more hyper-inflammatory response to LPS, but compromised pathogen response.					
34489976	1	39	theme	impaired	267:274	arg1	healing					282:288	impaired wound healing	267:288	increased susceptibility to bacterial and viral infection as well as impaired wound healing	198:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	6	40	from	macaques	1050:1057	arg1	monocytes					1024:1032	monocytes	1024:1032	monocytes from CHD female macaques	1024:1057	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	4	41	theme	macaque	750:756	arg1	model					758:762	a translational rhesus macaque model	727:762	a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity	727:869	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	7	42	theme	CHD	1385:1387	arg1	macaques					1389:1396	CHD macaques	1385:1396	CHD macaques	1385:1396	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	1	43	theme	risk	182:185	arg1	factor					187:192	a known risk factor	174:192	a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing	174:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	43	theme	risk	182:185	arg1	drinking					145:152	Chronic heavy drinking	131:152	Chronic heavy drinking (CHD) of alcohol	131:169	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	0	44	theme	Epigenetic	17:26	arg1	Reprogramming					44:56	Transcriptional, Epigenetic, and Functional Reprogramming	0:56	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates	0:93	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	1	45	theme	wound	276:280	arg1	healing					282:288	impaired wound healing	267:288	increased susceptibility to bacterial and viral infection as well as impaired wound healing	198:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	0	46	from	Primates	86:93	arg1	Reprogramming					44:56	Transcriptional, Epigenetic, and Functional Reprogramming	0:56	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates	0:93	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	3	47	theme	human	553:557	arg1	patterns					568:575	human drinking patterns	553:575	human drinking patterns	553:575	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	8	48	theme	skewed	1442:1447	arg1	subsets					1434:1440	monocyte subsets	1425:1440	monocyte subsets skewed towards inflammatory phenotypes	1425:1479	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	0	49	theme	Functional	33:42	arg1	Reprogramming					44:56	Transcriptional, Epigenetic, and Functional Reprogramming	0:56	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates	0:93	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	5	50	theme	blood	921:925	arg1	monocytes					927:935	blood monocytes	921:935	blood monocytes	921:935	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	7	51	theme	scRNA-Seq	1176:1184	arg1	analysis					1186:1193	scRNA-Seq analysis	1176:1193	scRNA-Seq analysis of purified monocytes	1176:1215	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	4	52	theme	drinking	838:845	arg1	patterns					847:854	human drinking patterns	832:854	human drinking patterns	832:854	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	3	53	with	patients	648:655	arg1	disorders					674:682	alcohol use disorders	662:682	alcohol use disorders	662:682	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	6	54	theme	female	1043:1048	arg1	macaques					1050:1057	CHD female macaques	1039:1057	CHD female macaques	1039:1057	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	2	55	theme	myeloid	398:404	arg1	macrophages					435:445	macrophages	435:445	macrophages	435:445	Evidence suggests that these defects are mediated by a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages, but the mechanisms remain poorly understood.					
34489976	2	55	theme	myeloid	398:404	arg1	cells					406:410	myeloid cells	398:410	myeloid cells	398:410	Evidence suggests that these defects are mediated by a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages, but the mechanisms remain poorly understood.					
34489976	2	55	theme	myeloid	398:404	arg1	monocytes					421:429	monocytes	421:429	monocytes	421:429	Evidence suggests that these defects are mediated by a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages, but the mechanisms remain poorly understood.					
34489976	8	56	theme	cytokine	1610:1617	arg1	pathways					1629:1636	cytokine signaling pathways	1610:1636	cytokine signaling pathways	1610:1636	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	3	57	theme	risk	631:634	arg1	factors					636:642	confounding risk factors	619:642	confounding risk factors	619:642	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	4	58	theme	human	832:836	arg1	patterns					847:854	human drinking patterns	832:854	human drinking patterns	832:854	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	1	59	theme	increased	198:206	arg1	susceptibility					208:221	increased susceptibility	198:221	increased susceptibility to bacterial and viral infection as well as impaired wound healing	198:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	7	60	theme	purified	1198:1205	arg1	monocytes					1207:1215	purified monocytes	1198:1215	purified monocytes	1198:1215	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	6	61	theme	CHD	1039:1041	arg1	macaques					1050:1057	CHD female macaques	1039:1057	CHD female macaques	1039:1057	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	7	62	theme	cells	1296:1300	arg1	accumulation					1280:1291	accumulation	1280:1291	accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques	1280:1396	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	3	63	theme	use	670:672	arg1	disorders					674:682	alcohol use disorders	662:682	alcohol use disorders	662:682	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	3	64	theme	behavior	581:588	arg1	comorbidities					601:613	comorbidities	601:613	comorbidities	601:613	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	3	64	theme	behavior	581:588	arg1	complexities					537:548	the complexities	533:548	the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders	533:682	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	3	64	theme	behavior	581:588	arg1	factors					636:642	confounding risk factors	619:642	confounding risk factors	619:642	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	1	65	theme	Chronic	131:137	arg1	CHD					155:157	CHD	155:157	CHD	155:157	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	65	theme	Chronic	131:137	arg1	drinking					145:152	Chronic heavy drinking	131:152	Chronic heavy drinking (CHD) of alcohol	131:169	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	65	theme	Chronic	131:137	arg1	factor					187:192	a known risk factor	174:192	a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing	174:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	7	66	theme	NFkB	1358:1361	arg1	pathway					1373:1379	NFkB signaling pathway	1358:1379	NFkB signaling pathway	1358:1379	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	7	66	theme	NFkB	1358:1361	arg1	inflammation					1344:1355	inflammation	1344:1355	inflammation (NFkB signaling pathway)	1344:1380	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	8	67	theme	monocyte	1425:1432	arg1	subsets					1434:1440	monocyte subsets	1425:1440	monocyte subsets skewed towards inflammatory phenotypes	1425:1479	The increased presence of monocyte subsets skewed towards inflammatory phenotypes was complemented by epigenetic analysis, which revealed higher accessibility of promoter regions that regulate genes involved in cytokine signaling pathways.					
34489976	7	68	theme	signaling	1363:1371	arg1	pathway					1373:1379	NFkB signaling pathway	1358:1379	NFkB signaling pathway	1358:1379	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	7	68	theme	signaling	1363:1371	arg1	inflammation					1344:1355	inflammation	1344:1355	inflammation (NFkB signaling pathway)	1344:1380	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	6	69	dep	ex	1102:1103	arg1	vivo					1105:1108	vivo	1105:1108	vivo	1105:1108	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	1	70	theme	heavy	139:143	arg1	CHD					155:157	CHD	155:157	CHD	155:157	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	70	theme	heavy	139:143	arg1	drinking					145:152	Chronic heavy drinking	131:152	Chronic heavy drinking (CHD) of alcohol	131:169	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	1	70	theme	heavy	139:143	arg1	factor					187:192	a known risk factor	174:192	a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing	174:288	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	4	71	theme	voluntary	767:775	arg1	self-administration					785:803	voluntary ethanol self-administration	767:803	voluntary ethanol self-administration	767:803	To overcome these challenges, we utilized a translational rhesus macaque model of voluntary ethanol self-administration that closely recapitulates human drinking patterns and chronicity.					
34489976	3	72	theme	confounding	619:629	arg1	factors					636:642	confounding risk factors	619:642	confounding risk factors	619:642	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	6	73	theme	LPS	1110:1112	arg1	stimulation					1114:1124	ex vivo LPS stimulation	1102:1124	ex vivo LPS stimulation	1102:1124	While monocytes from CHD female macaques generated a hyper-inflammatory response to ex vivo LPS stimulation, their response to E. coli was dampened.					
34489976	3	74	theme	alcohol	662:668	arg1	disorders					674:682	alcohol use disorders	662:682	alcohol use disorders	662:682	Our ability to study CHD is impacted by the complexities of human drinking patterns and behavior as well as comorbidities and confounding risk factors for patients with alcohol use disorders.					
34489976	5	75	theme	ethanol	997:1003	arg1	consumption					1005:1015	daily ethanol consumption	991:1015	daily ethanol consumption	991:1015	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	7	76	theme	hypoxia	1324:1330	arg1	markers					1313:1319	markers	1313:1319	markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway)	1313:1380	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
34489976	2	77	theme	inflammatory	359:370	arg1	response					372:379	a dysregulated inflammatory response	344:379	a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages	344:445	Evidence suggests that these defects are mediated by a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages, but the mechanisms remain poorly understood.					
34489976	1	78	theme	bacterial	226:234	arg1	infection					246:254	bacterial and viral infection	226:254	bacterial and viral infection	226:254	Chronic heavy drinking (CHD) of alcohol is a known risk factor for increased susceptibility to bacterial and viral infection as well as impaired wound healing.					
34489976	0	79	theme	Monocytes	61:69	arg1	Reprogramming					44:56	Transcriptional, Epigenetic, and Functional Reprogramming	0:56	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates	0:93	Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking.					
34489976	5	80	theme	consumption	1005:1015	arg1	months					981:986	12 months	978:986	12 months of daily ethanol consumption	978:1015	In this study, we examined the effects of CHD on blood monocytes in control and CHD female macaques after 12 months of daily ethanol consumption.					
34489976	2	81	theme	dysregulated	346:357	arg1	response					372:379	a dysregulated inflammatory response	344:379	a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages	344:445	Evidence suggests that these defects are mediated by a dysregulated inflammatory response originating from myeloid cells, notably monocytes and macrophages, but the mechanisms remain poorly understood.					
34489976	7	82	theme	monocytes	1207:1215	arg1	analysis					1186:1193	scRNA-Seq analysis	1176:1193	scRNA-Seq analysis of purified monocytes	1176:1215	In depth scRNA-Seq analysis of purified monocytes revealed significant shifts in classical monocyte subsets with accumulation of cells expressing markers of hypoxia (HIF1A) and inflammation (NFkB signaling pathway) in CHD macaques.					
33753088	6	0	theme	experimental	808:819	arg1	mice					821:824	experimental mice	808:824	experimental mice	808:824	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	12	1	theme	reduced	1493:1499	arg1	biodiversity					1501:1512	reduced biodiversity	1493:1512	reduced biodiversity	1493:1512	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	7	2	theme	flora	850:854	arg1	composition					831:841	The composition	827:841	The composition of gut flora	827:854	The composition of gut flora was evaluated by 16s rDNA sequencing.					
33753088	6	3	theme	primary	780:786	arg1	cells					788:792	primary cells	780:792	primary cells isolated from experimental mice	780:824	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	12	4	theme	Firmicutes	1458:1467	arg1	ratio					1483:1487	enhanced Firmicutes:Bacteriodetes ratio	1449:1487	enhanced Firmicutes:Bacteriodetes ratio	1449:1487	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	0	5	theme	systemic	120:127	arg1	inflammation					129:140	systemic inflammation	120:140	systemic inflammation	120:140	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	10	6	theme	decreased	1052:1060	arg1	mass					1067:1070	decreased bone mass	1052:1070	decreased bone mass	1052:1070	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	14	7	theme	HFD	1946:1948	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	7	theme	HFD	1946:1948	arg1	cytokines					2012:2020	inflammatory cytokines	1999:2020	inflammatory cytokines	1999:2020	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	7	theme	HFD	1946:1948	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	0	8	from	dysbiosis	76:84	arg1	mice					145:148	mice	145:148	mice	145:148	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	12	9	theme	microbiota	1413:1422	arg1	dysbiosis					1424:1432	microbiota dysbiosis	1413:1432	microbiota dysbiosis	1413:1432	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	3	10	theme	flora	428:432	arg1	improvement					402:412	The improvement	398:412	The improvement of intestinal flora by prebiotics	398:446	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	3	10	theme	flora	428:432	arg1	strategy					464:471	a promising strategy	452:471	a promising strategy for ameliorating obesity-related bone loss	452:514	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	13	11	theme	microbiota	1767:1776	arg1	dysbiosis					1778:1786	microbiota dysbiosis	1767:1786	microbiota dysbiosis	1767:1786	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	14	12	theme	flora	1937:1941	arg1	biodiversity					1886:1897	biodiversity	1886:1897	biodiversity	1886:1897	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	12	theme	flora	1937:1941	arg1	concentrations					1908:1921	SCFA concentrations	1903:1921	SCFA concentrations of intestinal flora	1903:1941	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	12	13	theme	inflammatory	1637:1648	arg1	IL6					1677:1679	IL6	1677:1679	IL6	1677:1679	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	13	theme	inflammatory	1637:1648	arg1	cytokines					1650:1658	inflammatory cytokines	1637:1658	inflammatory cytokines (including TNFα, IL6, and IL17)	1637:1690	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	13	theme	inflammatory	1637:1648	arg1	TNFα					1671:1674	TNFα	1671:1674	TNFα	1671:1674	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	13	theme	inflammatory	1637:1648	arg1	IL17					1686:1689	IL17	1686:1689	IL17	1686:1689	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	0	14	from	permeability	103:114	arg1	mice					145:148	mice	145:148	mice	145:148	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	14	15	dep	end	2030:2032	arg1	protect					2034:2040	protect	2034:2040	protect against HFD induced osteopenia	2034:2071	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	16	theme	SCFA	1903:1906	arg1	concentrations					1908:1921	SCFA concentrations	1903:1921	SCFA concentrations of intestinal flora	1903:1941	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	17	from	biodiversity	1886:1897	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	17	from	biodiversity	1886:1897	arg1	cytokines					2012:2020	inflammatory cytokines	1999:2020	inflammatory cytokines	1999:2020	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	17	from	biodiversity	1886:1897	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	13	18	theme	decreased	1741:1749	arg1	mass					1756:1759	decreased bone mass	1741:1759	decreased bone mass	1741:1759	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	4	19	theme	METHODS	517:523	arg1	model					533:537	METHODS Obesity model	517:537	METHODS Obesity model	517:537	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	0	20	theme	high	87:90	arg1	permeability					103:114	high intestinal permeability	87:114	high intestinal permeability	87:114	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	11	21	theme	HFD-induced	1232:1242	arg1	loss					1249:1252	HFD-induced bone loss	1232:1252	HFD-induced bone loss	1232:1252	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	13	22	theme	Long-term	1720:1728	arg1	HFD					1730:1732	CONCLUSION Long-term HFD	1709:1732	CONCLUSION Long-term HFD	1709:1732	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	2	23	theme	loss	392:395	arg1	pathogenesis					340:351	the pathogenesis	336:351	the pathogenesis of excessive fat accumulation and bone loss	336:395	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	10	24	theme	decreased	1107:1115	arg1	osteogenesis					1117:1128	decreased osteogenesis	1107:1128	decreased osteogenesis	1107:1128	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	14	25	theme	HFD	2050:2052	arg1	osteopenia					2062:2071	HFD induced osteopenia	2050:2071	HFD induced osteopenia	2050:2071	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	3	26	theme	intestinal	417:426	arg1	flora					428:432	intestinal flora	417:432	intestinal flora	417:432	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	3	27	theme	promising	454:462	arg1	improvement					402:412	The improvement	398:412	The improvement of intestinal flora by prebiotics	398:446	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	3	27	theme	promising	454:462	arg1	strategy					464:471	a promising strategy	452:471	a promising strategy for ameliorating obesity-related bone loss	452:514	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	0	28	theme	high-fat	19:26	arg1	diet					28:31	high-fat diet	19:31	high-fat diet induced bone loss	19:49	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	8	29	theme	junction	919:926	arg1	proteins					928:935	intestinal junction proteins	908:935	intestinal junction proteins	908:935	Expression of intestinal junction proteins was assessed by qPCR and immunohistochemistry.					
33753088	11	30	theme	adipocytes	1314:1323	arg1	differentiation					1282:1296	the imbalanced differentiation	1267:1296	the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts	1267:1340	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	12	31	dep	Firmicutes	1458:1467	arg1	Bacteriodetes					1469:1481	Bacteriodetes	1469:1481	Bacteriodetes	1469:1481	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	13	32	theme	CONCLUSION	1709:1718	arg1	HFD					1730:1732	CONCLUSION Long-term HFD	1709:1732	CONCLUSION Long-term HFD	1709:1732	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	12	33	theme	proteins	1564:1571	arg1	expression					1530:1539	expression	1530:1539	expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A)	1530:1620	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	14	34	theme	inflammatory	1999:2010	arg1	cytokines					2012:2020	inflammatory cytokines	1999:2020	inflammatory cytokines	1999:2020	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	34	theme	inflammatory	1999:2010	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	11	35	theme	osteoblasts	1301:1311	arg1	differentiation					1282:1296	the imbalanced differentiation	1267:1296	the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts	1267:1340	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	1	36	contain	have	217:220	arg1	BACKGROUND					151:160	BACKGROUND Obesity and osteoporosis	151:185	BACKGROUND Obesity and osteoporosis	151:185	BACKGROUND Obesity and osteoporosis frequently coexist, and might have a causal relationship.					
33753088	1	36	contain	have	217:220	arg2	relationship					231:242	a causal relationship	222:242	a causal relationship	222:242	BACKGROUND Obesity and osteoporosis frequently coexist, and might have a causal relationship.					
33753088	6	37	theme	osteoclastic	734:745	arg1	differentiation					747:761	Osteoblastic, adipocytic, and osteoclastic differentiation	704:761	Osteoblastic, adipocytic, and osteoclastic differentiation	704:761	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	1	38	theme	causal	224:229	arg1	relationship					231:242	a causal relationship	222:242	a causal relationship	222:242	BACKGROUND Obesity and osteoporosis frequently coexist, and might have a causal relationship.					
33753088	11	39	theme	FOS/GOS	1189:1195	arg1	treatment					1197:1205	FOS/GOS treatment	1189:1205	FOS/GOS treatment	1189:1205	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	2	40	theme	fat	366:368	arg1	accumulation					370:381	excessive fat accumulation	356:381	excessive fat accumulation	356:381	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	6	41	attach	isolated	794:801	arg1	mice					821:824	experimental mice	808:824	experimental mice	808:824	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	6	41	attach	isolated	794:801	arg2	cells					788:792	primary cells	780:792	primary cells isolated from experimental mice	780:824	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	14	42	theme	high	1973:1976	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	42	theme	high	1973:1976	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	9	43	theme	Cytokine	984:991	arg1	levels					993:998	Cytokine levels	984:998	Cytokine levels	984:998	Cytokine levels were measured by qPCR.					
33753088	12	44	theme	intestinal	1544:1553	arg1	ZO-1					1605:1608	ZO-1	1605:1608	ZO-1	1605:1608	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	44	theme	intestinal	1544:1553	arg1	JAM-A					1615:1619	JAM-A	1615:1619	JAM-A	1615:1619	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	44	theme	intestinal	1544:1553	arg1	Claudin15					1594:1602	Claudin15	1594:1602	Claudin15	1594:1602	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	44	theme	intestinal	1544:1553	arg1	proteins					1564:1571	intestinal junction proteins	1544:1571	intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A)	1544:1620	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	44	theme	intestinal	1544:1553	arg1	Claudin1					1584:1591	Claudin1	1584:1591	Claudin1	1584:1591	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	4	45	theme	fat	581:583	arg1	HFD					591:593	HFD	591:593	HFD	591:593	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	4	45	theme	fat	581:583	arg1	diet					585:588	high fat diet	576:588	high fat diet (HFD) for 16 weeks	576:607	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	13	46	theme	systemic	1804:1811	arg1	inflammation					1813:1824	systemic inflammation	1804:1824	systemic inflammation	1804:1824	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	2	47	theme	bone	292:295	arg1	metabolism					297:306	bone metabolism	292:306	bone metabolism	292:306	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	7	48	theme	rDNA	877:880	arg1	sequencing					882:891	rDNA sequencing	877:891	rDNA sequencing	877:891	The composition of gut flora was evaluated by 16s rDNA sequencing.					
33753088	3	49	theme	obesity-related	490:504	arg1	loss					511:514	obesity-related bone loss	490:514	obesity-related bone loss	490:514	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	4	50	with	mice	566:569	arg1	HFD					591:593	HFD	591:593	HFD	591:593	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	4	50	with	mice	566:569	arg1	diet					585:588	high fat diet	576:588	high fat diet (HFD) for 16 weeks	576:607	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	14	51	theme	reverse	1965:1971	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	51	theme	reverse	1965:1971	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	13	52	theme	leaky	1789:1793	arg1	gut					1795:1797	leaky gut	1789:1797	leaky gut	1789:1797	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	7	53	theme	gut	846:848	arg1	flora					850:854	gut flora	846:854	gut flora	846:854	The composition of gut flora was evaluated by 16s rDNA sequencing.					
33753088	14	54	theme	fed	1950:1952	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	54	theme	fed	1950:1952	arg1	cytokines					2012:2020	inflammatory cytokines	1999:2020	inflammatory cytokines	1999:2020	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	54	theme	fed	1950:1952	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	12	55	theme	enhanced	1449:1456	arg1	ratio					1483:1487	enhanced Firmicutes:Bacteriodetes ratio	1449:1487	enhanced Firmicutes:Bacteriodetes ratio	1449:1487	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	2	56	theme	Gut	245:247	arg1	microbiota					249:258	Gut microbiota	245:258	Gut microbiota	245:258	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	10	57	theme	Long-term	1031:1039	arg1	HFD					1041:1043	Long-term HFD	1031:1043	Long-term HFD	1031:1043	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	14	58	theme	intestinal	1926:1935	arg1	flora					1937:1941	intestinal flora	1926:1941	intestinal flora	1926:1941	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	0	59	theme	microbiota	65:74	arg1	dysbiosis					76:84	microbiota dysbiosis	65:84	microbiota dysbiosis	65:84	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	10	60	theme	bone	1062:1065	arg1	mass					1067:1070	decreased bone mass	1052:1070	decreased bone mass	1052:1070	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	12	61	theme	FOS/GOS	1378:1384	arg1	administration					1386:1399	FOS/GOS administration	1378:1399	FOS/GOS administration	1378:1399	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	4	62	theme	Obesity	525:531	arg1	model					533:537	METHODS Obesity model	517:537	METHODS Obesity model	517:537	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	13	63	theme	bone	1751:1754	arg1	mass					1756:1759	decreased bone mass	1741:1759	decreased bone mass	1741:1759	CONCLUSION Long-term HFD led to decreased bone mass, with microbiota dysbiosis, leaky gut, and systemic inflammation.					
33753088	0	64	theme	intestinal	92:101	arg1	permeability					103:114	high intestinal permeability	87:114	high intestinal permeability	87:114	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	11	65	theme	osteoclasts	1330:1340	arg1	differentiation					1282:1296	the imbalanced differentiation	1267:1296	the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts	1267:1340	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	6	66	theme	Osteoblastic	704:715	arg1	differentiation					747:761	Osteoblastic, adipocytic, and osteoclastic differentiation	704:761	Osteoblastic, adipocytic, and osteoclastic differentiation	704:761	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	14	67	theme	induced	2054:2060	arg1	osteopenia					2062:2071	HFD induced osteopenia	2050:2071	HFD induced osteopenia	2050:2071	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	12	68	theme	HFD-fed	1695:1701	arg1	mice					1703:1706	HFD-fed mice	1695:1706	HFD-fed mice	1695:1706	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	2	69	theme	bone	387:390	arg1	loss					392:395	bone loss	387:395	bone loss	387:395	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	10	70	dep	RESULTS	1023:1029	arg1	caused					1045:1050	caused	1045:1050	caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis	1045:1186	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	2	71	theme	accumulation	370:381	arg1	pathogenesis					340:351	the pathogenesis	336:351	the pathogenesis of excessive fat accumulation and bone loss	336:395	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	14	72	theme	FOS/GOS	1849:1855	arg1	administration					1831:1844	The administration	1827:1844	The administration of FOS/GOS	1827:1855	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	11	73	theme	bone	1244:1247	arg1	loss					1249:1252	HFD-induced bone loss	1232:1252	HFD-induced bone loss	1232:1252	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	4	74	theme	high	576:579	arg1	HFD					591:593	HFD	591:593	HFD	591:593	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	4	74	theme	high	576:579	arg1	diet					585:588	high fat diet	576:588	high fat diet (HFD) for 16 weeks	576:607	METHODS Obesity model was established by feeding mice with high fat diet (HFD) for 16 weeks.					
33753088	1	75	dep	BACKGROUND	151:160	arg1	osteoporosis					174:185	osteoporosis	174:185	osteoporosis	174:185	BACKGROUND Obesity and osteoporosis frequently coexist, and might have a causal relationship.					
33753088	1	75	dep	BACKGROUND	151:160	arg1	Obesity					162:168	Obesity	162:168	Obesity	162:168	BACKGROUND Obesity and osteoporosis frequently coexist, and might have a causal relationship.					
33753088	0	76	theme	bone	41:44	arg1	loss					46:49	bone loss	41:49	bone loss	41:49	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	10	77	theme	increased	1131:1139	arg1	osteoclastogenesis					1141:1158	increased osteoclastogenesis	1131:1158	increased osteoclastogenesis	1131:1158	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	8	78	theme	intestinal	908:917	arg1	proteins					928:935	intestinal junction proteins	908:935	intestinal junction proteins	908:935	Expression of intestinal junction proteins was assessed by qPCR and immunohistochemistry.					
33753088	11	79	theme	imbalanced	1271:1280	arg1	differentiation					1282:1296	the imbalanced differentiation	1267:1296	the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts	1267:1340	FOS/GOS treatment significantly alleviated HFD-induced bone loss and reversed the imbalanced differentiation of osteoblasts, adipocytes, and osteoclasts.					
33753088	12	80	dep	ameliorated	1401:1411	arg1	manifested					1435:1444	manifested	1435:1444	manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity	1435:1512	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	8	81	theme	proteins	928:935	arg1	Expression					894:903	Expression	894:903	Expression of intestinal junction proteins	894:935	Expression of intestinal junction proteins was assessed by qPCR and immunohistochemistry.					
33753088	3	82	theme	bone	506:509	arg1	loss					511:514	obesity-related bone loss	490:514	obesity-related bone loss	490:514	The improvement of intestinal flora by prebiotics was a promising strategy for ameliorating obesity-related bone loss.					
33753088	14	83	from	concentrations	1908:1921	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	83	from	concentrations	1908:1921	arg1	cytokines					2012:2020	inflammatory cytokines	1999:2020	inflammatory cytokines	1999:2020	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	83	from	concentrations	1908:1921	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	10	84	theme	excessive	1165:1173	arg1	adipogenesis					1175:1186	excessive adipogenesis	1165:1186	excessive adipogenesis	1165:1186	RESULTS Long-term HFD caused decreased bone mass in mice, which was associated with decreased osteogenesis, increased osteoclastogenesis, and excessive adipogenesis.					
33753088	12	85	theme	junction	1555:1562	arg1	ZO-1					1605:1608	ZO-1	1605:1608	ZO-1	1605:1608	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	85	theme	junction	1555:1562	arg1	JAM-A					1615:1619	JAM-A	1615:1619	JAM-A	1615:1619	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	85	theme	junction	1555:1562	arg1	Claudin15					1594:1602	Claudin15	1594:1602	Claudin15	1594:1602	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	85	theme	junction	1555:1562	arg1	proteins					1564:1571	intestinal junction proteins	1544:1571	intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A)	1544:1620	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	12	85	theme	junction	1555:1562	arg1	Claudin1					1584:1591	Claudin1	1584:1591	Claudin1	1584:1591	In addition, our study showed that FOS/GOS administration ameliorated microbiota dysbiosis (manifested as enhanced Firmicutes:Bacteriodetes ratio and reduced biodiversity), downregulated expression of intestinal junction proteins (including Claudin1, Claudin15, ZO-1, and JAM-A), and increased inflammatory cytokines (including TNFα, IL6, and IL17) in HFD-fed mice.					
33753088	2	86	theme	important	318:326	arg1	role					328:331	an important role	315:331	an important role	315:331	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
33753088	14	87	theme	gut	1978:1980	arg1	permeability					1982:1993	reverse high gut permeability	1965:1993	reverse high gut permeability	1965:1993	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	14	87	theme	gut	1978:1980	arg1	mice					1954:1957	HFD fed mice	1946:1957	HFD fed mice	1946:1957	The administration of FOS/GOS could significantly increase biodiversity and SCFA concentrations of intestinal flora in HFD fed mice, then reverse high gut permeability and inflammatory cytokines, in the end protect against HFD induced osteopenia.					
33753088	0	88	from	inflammation	129:140	arg1	mice					145:148	mice	145:148	mice	145:148	FOS/GOS attenuates high-fat diet induced bone loss via reversing microbiota dysbiosis, high intestinal permeability and systemic inflammation in mice.					
33753088	6	89	theme	adipocytic	718:727	arg1	differentiation					747:761	Osteoblastic, adipocytic, and osteoclastic differentiation	704:761	Osteoblastic, adipocytic, and osteoclastic differentiation	704:761	Osteoblastic, adipocytic, and osteoclastic differentiation was performed on primary cells isolated from experimental mice.					
33753088	2	90	theme	excessive	356:364	arg1	accumulation					370:381	excessive fat accumulation	356:381	excessive fat accumulation	356:381	Gut microbiota, associated with both lipid and bone metabolism, plays an important role in the pathogenesis of excessive fat accumulation and bone loss.					
32069798	5	0	theme	component	790:798	arg1	polysaccharides					808:822	the component anionic polysaccharides	786:822	the component anionic polysaccharides	786:822	It was revealed that the component anionic polysaccharides affected the film's properties such as the swelling behavior and mechanical characteristics.					
32069798	6	1	dep	cells	988:992	arg1	integrin					995:1002	integrin	995:1002	integrin	995:1002	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	1	dep	cells	988:992	arg1	CD44					1011:1014	CD44	1011:1014	CD44	1011:1014	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	11	2	theme	polysaccharide	1794:1807	arg1	rates					1821:1825	the anionic polysaccharide composition rates	1782:1825	the anionic polysaccharide composition rates	1782:1825	These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.					
32069798	2	3	theme	composite	393:401	arg1	films					403:407	polysaccharide composite films	378:407	polysaccharide composite films	378:407	This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components.					
32069798	5	4	theme	anionic	800:806	arg1	polysaccharides					808:822	the component anionic polysaccharides	786:822	the component anionic polysaccharides	786:822	It was revealed that the component anionic polysaccharides affected the film's properties such as the swelling behavior and mechanical characteristics.					
32069798	2	5	theme	polysaccharide	378:391	arg1	films					403:407	polysaccharide composite films	378:407	polysaccharide composite films	378:407	This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components.					
32069798	6	6	dep	integrin	943:950	arg1	lack					965:968	lack	965:968	lack	965:968	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	6	dep	integrin	943:950	arg1	high					953:956	high	953:956	high	953:956	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	6	dep	integrin	943:950	arg1	weak					973:976	weak	973:976	weak	973:976	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	7	dep	integrin	995:1002	arg1	high					1017:1020	high	1017:1020	high	1017:1020	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	7	dep	integrin	995:1002	arg1	high					1005:1008	high	1005:1008	high	1005:1008	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	7	8	theme	A549	1077:1080	arg1	cells					1082:1086	Both NIH3T3 and A549 cells	1061:1086	cells	1082:1086	Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin.					
32069798	8	9	theme	/CHI	1278:1281	arg1	films					1283:1287	hyaluronic acid (HYA)/CHI films	1257:1287	hyaluronic acid (HYA)/CHI films	1257:1287	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	1	10	theme	natural	120:126	arg1	polysaccharides					128:142	natural polysaccharides	120:142	natural polysaccharides	120:142	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	8	11	theme	NIH3T3	1298:1303	arg1	cells					1305:1309	NIH3T3 cells	1298:1309	NIH3T3 cells	1298:1309	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	10	12	theme	polysaccharides	1641:1655	arg1	polysaccharide					1601:1614	one cationic polysaccharide	1588:1614	one cationic polysaccharide	1588:1614	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	10	12	theme	polysaccharides	1641:1655	arg1	kinds					1624:1628	two kinds	1620:1628	two kinds of anionic polysaccharides	1620:1655	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	10	13	dep	films	1538:1542	arg1	polysaccharide					1601:1614	one cationic polysaccharide	1588:1614	one cationic polysaccharide	1588:1614	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	10	13	dep	films	1538:1542	arg1	kinds					1624:1628	two kinds	1620:1628	two kinds of anionic polysaccharides	1620:1655	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	10	14	theme	anionic	1633:1639	arg1	polysaccharides					1641:1655	anionic polysaccharides	1633:1655	anionic polysaccharides	1633:1655	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	1	15	theme	specific	283:290	arg1	biodegradability					255:270	biodegradability	255:270	biodegradability	255:270	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	15	theme	specific	283:290	arg1	functions					303:311	their specific biological functions	277:311	their specific biological functions based on their chemical structures	277:346	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	15	theme	specific	283:290	arg1	biocompatibility					234:249	high biocompatibility	229:249	high biocompatibility	229:249	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	2	16	with	complexes	440:448	arg1	particles					468:476	their particles	462:476	their particles used as building components	462:504	This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components.					
32069798	2	17	theme	polyion	432:438	arg1	PICs					451:454	PICs	451:454	PICs	451:454	This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components.					
32069798	2	17	theme	polyion	432:438	arg1	complexes					440:448	polyion complexes	432:448	polyion complexes (PICs) with their particles used as building components	432:504	This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components.					
32069798	6	18	theme	cells	988:992	arg1	Adhesion					917:924	Adhesion	917:924	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films	917:1044	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	19	theme	NIH3T3	929:934	arg1	cells					936:940	NIH3T3 cells	929:940	NIH3T3 cells (integrin: high, CD44: lack or weak)	929:977	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	7	20	theme	HEP/CHI	1117:1123	arg1	film					1126:1129	heparin/chitosan (HEP/CHI) film	1099:1129	heparin/chitosan (HEP/CHI) film	1099:1129	Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin.					
32069798	9	21	theme	anionic	1425:1431	arg1	polysaccharides					1433:1447	anionic polysaccharides	1425:1447	anionic polysaccharides	1425:1447	These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films.					
32069798	6	22	theme	A549	983:986	arg1	cells					988:992	A549 cells	983:992	A549 cells (integrin: high, CD44: high)	983:1021	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	3	23	theme	solutions	599:607	arg1	centrifugation					555:568	centrifugation	555:568	centrifugation	555:568	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	3	23	theme	solutions	599:607	arg1	re-dispersion					575:587	re-dispersion	575:587	re-dispersion	575:587	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	3	23	theme	solutions	599:607	arg1	mixing					547:552	mixing	547:552	mixing	547:552	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	6	24	theme	cells	936:940	arg1	Adhesion					917:924	Adhesion	917:924	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films	917:1044	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	8	25	contain	have	1344:1347	arg2	affinity					1349:1356	affinity	1349:1356	affinity for CD44	1349:1365	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	8	25	contain	have	1344:1347	arg1	HYA					1340:1342	HYA	1340:1342	HYA	1340:1342	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	8	25	contain	have	1344:1347	arg1	CS					1333:1334	CS	1333:1334	CS	1333:1334	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	1	26	theme	biological	292:301	arg1	biodegradability					255:270	biodegradability	255:270	biodegradability	255:270	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	26	theme	biological	292:301	arg1	functions					303:311	their specific biological functions	277:311	their specific biological functions based on their chemical structures	277:346	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	26	theme	biological	292:301	arg1	biocompatibility					234:249	high biocompatibility	229:249	high biocompatibility	229:249	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	4	27	theme	water	748:752	arg1	insoluble					754:762	water insoluble	748:762	water insoluble	748:762	These films were homogenous and water insoluble.					
32069798	5	28	theme	mechanical	889:898	arg1	characteristics					900:914	mechanical characteristics	889:914	mechanical characteristics	889:914	It was revealed that the component anionic polysaccharides affected the film's properties such as the swelling behavior and mechanical characteristics.					
32069798	9	29	theme	composite	1487:1495	arg1	films					1497:1501	the composite films	1483:1501	the composite films	1483:1501	These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films.					
32069798	1	30	theme	excellent	199:207	arg1	biodegradability					255:270	biodegradability	255:270	biodegradability	255:270	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	30	theme	excellent	199:207	arg1	biocompatibility					234:249	high biocompatibility	229:249	high biocompatibility	229:249	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	30	theme	excellent	199:207	arg1	properties					209:218	their excellent properties	193:218	their excellent properties	193:218	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	1	31	theme	functions	303:311	arg1	fact					185:188	the fact	181:188	the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures	181:346	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	9	32	theme	films	1497:1501	arg1	surface					1472:1478	the surface	1468:1478	the surface of the composite films	1468:1501	These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films.					
32069798	1	33	theme	properties	209:218	arg1	fact					185:188	the fact	181:188	the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures	181:346	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	3	34	theme	dilute	592:597	arg1	solutions					599:607	dilute solutions	592:607	dilute solutions	592:607	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	5	35	theme	swelling	867:874	arg1	behavior					876:883	the swelling behavior	863:883	the swelling behavior	863:883	It was revealed that the component anionic polysaccharides affected the film's properties such as the swelling behavior and mechanical characteristics.					
32069798	0	36	theme	Composite	51:59	arg1	Films					61:65	Polysaccharide Composite Films	36:65	Polysaccharide Composite Films	36:65	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	9	37	theme	biological	1401:1410	arg1	functions					1412:1420	the biological functions	1397:1420	the biological functions of anionic polysaccharides	1397:1447	These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films.					
32069798	10	38	theme	cationic	1592:1599	arg1	polysaccharide					1601:1614	one cationic polysaccharide	1588:1614	one cationic polysaccharide	1588:1614	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	0	39	from	Particles	88:96	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	0	39	from	Particles	88:96	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	3	40	theme	anionic	625:631	arg1	polysaccharides					633:647	cationic and anionic polysaccharides	612:647	cationic and anionic polysaccharides	612:647	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	6	41	theme	composite	1030:1038	arg1	films					1040:1044	the composite films	1026:1044	the composite films	1026:1044	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	7	42	theme	heparin/chitosan	1099:1114	arg1	film					1126:1129	heparin/chitosan (HEP/CHI) film	1099:1129	heparin/chitosan (HEP/CHI) film	1099:1129	Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin.					
32069798	0	43	theme	Polysaccharide	36:49	arg1	Films					61:65	Polysaccharide Composite Films	36:65	Polysaccharide Composite Films	36:65	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	11	44	theme	films	1700:1704	arg1	properties					1686:1695	the properties	1682:1695	the properties of films composed of three kinds of polysaccharides	1682:1747	These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.					
32069798	11	44	theme	films	1700:1704	arg1	controllable					1756:1767	controllable	1756:1767	controllable	1756:1767	These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.					
32069798	8	45	theme	hyaluronic	1257:1266	arg1	acid					1268:1271	hyaluronic acid	1257:1271	hyaluronic acid (HYA)/CHI films	1257:1287	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	8	45	theme	hyaluronic	1257:1266	arg1	HYA					1274:1276	HYA	1274:1276	HYA	1274:1276	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	3	46	theme	polysaccharides	633:647	arg1	centrifugation					555:568	centrifugation	555:568	centrifugation	555:568	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	3	46	theme	polysaccharides	633:647	arg1	re-dispersion					575:587	re-dispersion	575:587	re-dispersion	575:587	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	3	46	theme	polysaccharides	633:647	arg1	mixing					547:552	mixing	547:552	mixing	547:552	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	8	47	theme	sulfate	1236:1242	arg1	/CHI					1248:1251	chondroitin sulfate (CS)/CHI	1224:1251	chondroitin sulfate (CS)/CHI	1224:1251	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	2	48	theme	building	486:493	arg1	components					495:504	building components	486:504	building components	486:504	This study demonstrates that polysaccharide composite films can be fabricated from polyion complexes (PICs) with their particles used as building components.					
32069798	1	49	theme	chemical	328:335	arg1	structures					337:346	their chemical structures	322:346	their chemical structures	322:346	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	11	50	theme	composition	1809:1819	arg1	rates					1821:1825	the anionic polysaccharide composition rates	1782:1825	the anionic polysaccharide composition rates	1782:1825	These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.					
32069798	3	51	theme	cationic	612:619	arg1	polysaccharides					633:647	cationic and anionic polysaccharides	612:647	cationic and anionic polysaccharides	612:647	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	3	52	theme	PIC	521:523	arg1	particles					525:533	PIC particles	521:533	PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides	521:647	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	0	53	theme	Films	61:65	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	0	53	theme	Films	61:65	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	3	54	theme	particles	525:533	arg1	Dispersion					507:516	Dispersion	507:516	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides	507:647	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	7	55	contain	has	1143:1145	arg1	HEP					1139:1141	HEP	1139:1141	HEP	1139:1141	Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin.					
32069798	7	55	contain	has	1143:1145	arg2	affinity					1150:1157	an affinity	1147:1157	an affinity for integrin	1147:1170	Both NIH3T3 and A549 cells adhered to heparin/chitosan (HEP/CHI) film because HEP has an affinity for integrin through fibronectin.					
32069798	11	56	theme	polysaccharides	1733:1747	arg1	kinds					1724:1728	three kinds	1718:1728	three kinds of polysaccharides	1718:1747	These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.					
32069798	3	57	theme	thick	709:713	arg1	films					683:687	films	683:687	films several micrometers thick	683:713	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	11	58	theme	anionic	1786:1792	arg1	rates					1821:1825	the anionic polysaccharide composition rates	1782:1825	the anionic polysaccharide composition rates	1782:1825	These results show that the properties of films composed of three kinds of polysaccharides may be controllable depending on the anionic polysaccharide composition rates.					
32069798	9	59	theme	polysaccharides	1433:1447	arg1	functions					1412:1420	the biological functions	1397:1420	the biological functions of anionic polysaccharides	1397:1447	These results indicated that the biological functions of anionic polysaccharides were maintained on the surface of the composite films.					
32069798	1	60	theme	high	229:232	arg1	biocompatibility					234:249	high biocompatibility	229:249	high biocompatibility	229:249	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	10	61	theme	polysaccharides	1571:1585	arg1	kinds					1562:1566	three kinds	1556:1566	three kinds of polysaccharides	1556:1585	It was also possible to fabricate films composed of three kinds of polysaccharides: one cationic polysaccharide and two kinds of anionic polysaccharides.					
32069798	1	62	theme	much	159:162	arg1	attention					164:172	much attention	159:172	much attention	159:172	Biomaterials made of natural polysaccharides have attracted much attention due to the fact of their excellent properties, such as high biocompatibility and biodegradability, and their specific biological functions based on their chemical structures.					
32069798	0	63	theme	Complex	80:86	arg1	Particles					88:96	Polyion Complex Particles	72:96	Polyion Complex Particles	72:96	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	3	64	theme	several	689:695	arg1	micrometers					697:707	several micrometers	689:707	several micrometers	689:707	Dispersion of PIC particles prepared by mixing, centrifugation, and re-dispersion of dilute solutions of cationic and anionic polysaccharides were cast, dried, and formed into films several micrometers thick.					
32069798	8	65	theme	chondroitin	1224:1234	arg1	CS					1245:1246	CS	1245:1246	CS	1245:1246	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	8	65	theme	chondroitin	1224:1234	arg1	sulfate					1236:1242	chondroitin sulfate	1224:1242	chondroitin sulfate (CS)/CHI	1224:1251	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	8	66	theme	acid	1268:1271	arg1	films					1283:1287	hyaluronic acid (HYA)/CHI films	1257:1287	hyaluronic acid (HYA)/CHI films	1257:1287	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	0	67	theme	Polyion	72:78	arg1	Particles					88:96	Polyion Complex Particles	72:96	Polyion Complex Particles	72:96	Fabrication and Characterization of Polysaccharide Composite Films from Polyion Complex Particles.					
32069798	8	68	theme	A549	1202:1205	arg1	cells					1207:1211	A549 cells	1202:1211	A549 cells	1202:1211	However, A549 cells adhered to chondroitin sulfate (CS)/CHI and hyaluronic acid (HYA)/CHI films, whereas NIH3T3 cells did not, because both CS and HYA have affinity for CD44.					
32069798	6	69	dep	cells	936:940	arg1	CD44					959:962	CD44	959:962	CD44	959:962	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
32069798	6	69	dep	cells	936:940	arg1	integrin					943:950	integrin	943:950	integrin	943:950	Adhesion of NIH3T3 cells (integrin: high, CD44: lack or weak) and A549 cells (integrin: high, CD44: high) to the composite films were examined.					
33573977	14	0	theme	dietary	2190:2196	arg1	SBO					2198:2200	dietary SBO	2190:2200	dietary SBO	2190:2200	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	11	1	theme	Deep	1652:1655	arg1	sequencing					1657:1666	Deep sequencing	1652:1666	Deep sequencing of 16S rRNA	1652:1678	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	4	2	theme	basal	807:811	arg1	diet					813:816	basal diet	807:816	basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively)	807:915	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	6	3	theme	rRNA	1066:1069	arg1	sequencing					1071:1080	16S rRNA sequencing	1062:1080	16S rRNA sequencing	1062:1080	Caecum content was collected for the caecal microbiota analysis through 16S rRNA sequencing.					
33573977	7	4	theme	NC-fed	1270:1275	arg1	broilers					1277:1284	the NC-fed broilers	1266:1284	the NC-fed broilers	1266:1284	Results showed that on day 42, the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO and PC was significantly decreased (P < 0.01) compared with that in the NC-fed broilers.					
33573977	14	5	theme	odour-causing	2252:2264	arg1	compounds					2266:2274	odour-causing compounds	2252:2274	odour-causing compounds	2252:2274	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	6	6	theme	microbiota	1034:1043	arg1	analysis					1045:1052	the caecal microbiota analysis	1023:1052	the caecal microbiota analysis	1023:1052	Caecum content was collected for the caecal microbiota analysis through 16S rRNA sequencing.					
33573977	3	7	theme	broiler	553:559	arg1	chickens					561:568	One-day-old broiler chickens	541:568	One-day-old broiler chickens	541:568	One-day-old broiler chickens were subjected to a 42-day experiment involving 6 treatments with 6 replicates per treatment (10 birds/cage).					
33573977	13	8	theme	Rikenella	2129:2137	arg1	abundance					2116:2124	the abundance	2112:2124	the abundance of Rikenella	2112:2137	By contrast, the CHL supplementation demonstrated a strong tendency to enrich Ruminococcus and to reduce the abundance of Rikenella.					
33573977	2	9	theme	broiler	523:529	arg1	chickens					531:538	broiler chickens	523:538	broiler chickens	523:538	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	10	10	theme	acetate	1578:1584	arg1	P = 0.003					1601:1609	P = 0.003	1601:1609	P = 0.003	1601:1609	Similarly, acetate concentration (P = 0.003) was increased in birds fed with 3.5SBO.					
33573977	10	10	theme	acetate	1578:1584	arg1	concentration					1586:1598	acetate concentration	1578:1598	acetate concentration (P = 0.003)	1578:1610	Similarly, acetate concentration (P = 0.003) was increased in birds fed with 3.5SBO.					
33573977	4	11	dep	PC	765:766	arg1	diet					775:778	basal diet	769:778	basal diet supplemented with CHL	769:800	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	8	12	theme	excreta	1291:1297	arg1	concentration					1307:1319	The excreta skatole concentration	1287:1319	The excreta skatole concentration (P < 0.001)	1287:1331	The excreta skatole concentration (P < 0.001) and pH (P < 0.05) were also decreased by SBO and CHL, and they were lowest in birds fed with 3.5SBO.					
33573977	8	12	theme	excreta	1291:1297	arg1	P < 0.001					1322:1330	P < 0.001	1322:1330	P < 0.001	1322:1330	The excreta skatole concentration (P < 0.001) and pH (P < 0.05) were also decreased by SBO and CHL, and they were lowest in birds fed with 3.5SBO.					
33573977	2	13	with	chlortetracycline	415:431	arg1	amounts					361:367	different amounts	351:367	different amounts of dietary soybean oligosaccharides (SBO)	351:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	13	with	chlortetracycline	415:431	arg1	SBO					406:408	SBO	406:408	SBO	406:408	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	13	with	chlortetracycline	415:431	arg1	oligosaccharides					388:403	dietary soybean oligosaccharides	372:403	dietary soybean oligosaccharides (SBO)	372:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	7	14	dep	decreased	1223:1231	arg1	P < 0.01					1234:1241	P < 0.01	1234:1241	P < 0.01	1234:1241	Results showed that on day 42, the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO and PC was significantly decreased (P < 0.01) compared with that in the NC-fed broilers.					
33573977	11	15	theme	microbial	1724:1732	arg1	digesta					1734:1740	the caecal microbial digesta	1713:1740	the caecal microbial digesta	1713:1740	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	1	16	theme	odour	124:128	arg1	emissions					130:138	odour emissions	124:138	odour emissions	124:138	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	8	17	theme	skatole	1299:1305	arg1	concentration					1307:1319	The excreta skatole concentration	1287:1319	The excreta skatole concentration (P < 0.001)	1287:1331	The excreta skatole concentration (P < 0.001) and pH (P < 0.05) were also decreased by SBO and CHL, and they were lowest in birds fed with 3.5SBO.					
33573977	8	17	theme	skatole	1299:1305	arg1	P < 0.001					1322:1330	P < 0.001	1322:1330	P < 0.001	1322:1330	The excreta skatole concentration (P < 0.001) and pH (P < 0.05) were also decreased by SBO and CHL, and they were lowest in birds fed with 3.5SBO.					
33573977	9	18	theme	other	1542:1546	arg1	P < 0.001					1555:1563	P < 0.001	1555:1563	P < 0.001	1555:1563	The formate concentrations in birds fed with 3.5SBO and 5.0SBO were higher than those in birds fed with the other diets (P < 0.001).					
33573977	9	18	theme	other	1542:1546	arg1	diets					1548:1552	the other diets	1538:1552	the other diets (P < 0.001)	1538:1564	The formate concentrations in birds fed with 3.5SBO and 5.0SBO were higher than those in birds fed with the other diets (P < 0.001).					
33573977	3	19	theme	42-day	590:595	arg1	experiment					597:606	a 42-day experiment	588:606	a 42-day experiment involving 6 treatments with 6 replicates per treatment (10 birds/cage)	588:677	One-day-old broiler chickens were subjected to a 42-day experiment involving 6 treatments with 6 replicates per treatment (10 birds/cage).					
33573977	0	20	theme	caecal	80:85	arg1	microbiota					87:96	the caecal microbiota	76:96	the caecal microbiota in broilers	76:108	Soybean oligosaccharides attenuate odour compounds in excreta by modulating the caecal microbiota in broilers.					
33573977	14	21	from	composition	2302:2312	arg1	chickens					2346:2353	broiler chickens	2338:2353	broiler chickens	2338:2353	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	5	22	theme	compounds	971:979	arg1	analysis					953:960	the analysis	949:960	the analysis of odour compounds	949:979	Fresh excreta were sampled for the analysis of odour compounds by HPLC.					
33573977	7	23	from	PC	1202:1203	arg1	broilers					1154:1161	the broilers	1150:1161	the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO	1150:1196	Results showed that on day 42, the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO and PC was significantly decreased (P < 0.01) compared with that in the NC-fed broilers.					
33573977	4	24	theme	basal	734:738	arg1	diet					740:743	basal diet	734:743	basal diet	734:743	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	2	25	theme	soybean	380:386	arg1	SBO					406:408	SBO	406:408	SBO	406:408	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	25	theme	soybean	380:386	arg1	oligosaccharides					388:403	dietary soybean oligosaccharides	372:403	dietary soybean oligosaccharides (SBO)	372:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	11	26	theme	16S	1671:1673	arg1	rRNA					1675:1678	16S rRNA	1671:1678	16S rRNA	1671:1678	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	0	27	theme	Soybean	0:6	arg1	oligosaccharides					8:23	Soybean oligosaccharides	0:23	Soybean oligosaccharides	0:23	Soybean oligosaccharides attenuate odour compounds in excreta by modulating the caecal microbiota in broilers.					
33573977	4	28	dep	were	695:698	arg1	NC					730:731	NC	730:731	NC	730:731	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	28	dep	were	695:698	arg1	diet					813:816	basal diet	807:816	basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively)	807:915	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	28	dep	were	695:698	arg1	control					721:727	negative control	712:727	negative control (NC; basal diet)	712:744	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	28	dep	were	695:698	arg1	PC					765:766	PC	765:766	PC	765:766	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	28	dep	were	695:698	arg1	control					756:762	positive control	747:762	positive control (PC; basal diet supplemented with CHL)	747:801	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	2	29	theme	chlortetracycline	415:431	arg1	effects					319:325	the effects	315:325	the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens	315:538	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	14	30	with	supplementation	2169:2183	arg1	SBO					2198:2200	dietary SBO	2190:2200	dietary SBO	2190:2200	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	1	31	theme	environment	266:276	arg1	benefit					251:257	the benefit	247:257	the benefit of the environment	247:276	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	13	32	theme	CHL	2024:2026	arg1	supplementation					2028:2042	the CHL supplementation	2020:2042	the CHL supplementation	2020:2042	By contrast, the CHL supplementation demonstrated a strong tendency to enrich Ruminococcus and to reduce the abundance of Rikenella.					
33573977	2	33	theme	major	446:450	arg1	compounds					466:474	the major odour-causing compounds	442:474	the major odour-causing compounds in the excreta	442:489	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	34	with	supplementation	330:344	arg1	amounts					361:367	different amounts	351:367	different amounts of dietary soybean oligosaccharides (SBO)	351:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	34	with	supplementation	330:344	arg1	SBO					406:408	SBO	406:408	SBO	406:408	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	34	with	supplementation	330:344	arg1	oligosaccharides					388:403	dietary soybean oligosaccharides	372:403	dietary soybean oligosaccharides (SBO)	372:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	5	35	theme	Fresh	918:922	arg1	excreta					924:930	Fresh excreta	918:930	Fresh excreta	918:930	Fresh excreta were sampled for the analysis of odour compounds by HPLC.					
33573977	1	36	dep	quality	189:195	arg1	the					185:187	the	185:187	the	185:187	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	1	37	from	Abatement	111:119	arg1	production					151:160	poultry production	143:160	poultry production	143:160	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	1	38	theme	poultry	215:221	arg1	industry					223:230	the poultry industry	211:230	the poultry industry	211:230	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	14	39	theme	caecal	2317:2322	arg1	microbiota					2324:2333	caecal microbiota	2317:2333	caecal microbiota	2317:2333	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	6	40	theme	Caecum	990:995	arg1	content					997:1003	Caecum content	990:1003	Caecum content	990:1003	Caecum content was collected for the caecal microbiota analysis through 16S rRNA sequencing.					
33573977	4	41	theme	positive	747:754	arg1	PC					765:766	PC	765:766	PC	765:766	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	41	theme	positive	747:754	arg1	control					756:762	positive control	747:762	positive control (PC; basal diet supplemented with CHL)	747:801	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	2	42	theme	different	351:359	arg1	amounts					361:367	different amounts	351:367	different amounts of dietary soybean oligosaccharides (SBO)	351:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	42	theme	different	351:359	arg1	SBO					406:408	SBO	406:408	SBO	406:408	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	42	theme	different	351:359	arg1	oligosaccharides					388:403	dietary soybean oligosaccharides	372:403	dietary soybean oligosaccharides (SBO)	372:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	12	43	dep	Bilophilaand	1900:1911	arg1	Escherichia					1913:1923	Bacteroides, Bilophilaand Escherichia	1887:1923	Escherichia	1913:1923	The SBO supplementation decreased the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta.					
33573977	7	44	theme	indole	1126:1131	arg1	concentration					1133:1145	the excreta indole concentration	1114:1145	the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO	1114:1196	Results showed that on day 42, the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO and PC was significantly decreased (P < 0.01) compared with that in the NC-fed broilers.					
33573977	2	45	theme	supplementation	330:344	arg1	effects					319:325	the effects	315:325	the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens	315:538	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	3	46	theme	One-day-old	541:551	arg1	chickens					561:568	One-day-old broiler chickens	541:568	One-day-old broiler chickens	541:568	One-day-old broiler chickens were subjected to a 42-day experiment involving 6 treatments with 6 replicates per treatment (10 birds/cage).					
33573977	2	47	from	effects	319:325	arg1	microbiota					509:518	the caecal microbiota	498:518	the caecal microbiota in broiler chickens	498:538	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	47	from	effects	319:325	arg1	compounds					466:474	the major odour-causing compounds	442:474	the major odour-causing compounds in the excreta	442:489	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	6	48	theme	16S	1062:1064	arg1	sequencing					1071:1080	16S rRNA sequencing	1062:1080	16S rRNA sequencing	1062:1080	Caecum content was collected for the caecal microbiota analysis through 16S rRNA sequencing.					
33573977	14	49	theme	compounds	2266:2274	arg1	concentration					2235:2247	the concentration	2231:2247	the concentration of odour-causing compounds	2231:2274	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	4	50	theme	basal	769:773	arg1	diet					775:778	basal diet	769:778	basal diet supplemented with CHL	769:800	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	7	51	from	concentration	1133:1145	arg1	broilers					1154:1161	the broilers	1150:1161	the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO	1150:1196	Results showed that on day 42, the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO and PC was significantly decreased (P < 0.01) compared with that in the NC-fed broilers.					
33573977	12	52	theme	Bilophilaand	1900:1911	arg1	abundance					1874:1882	the abundance	1870:1882	the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta	1870:2004	The SBO supplementation decreased the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta.					
33573977	4	53	dep	diet	813:816	arg1	5.0SBO					895:900	5.0SBO	895:900	5.0SBO	895:900	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	53	dep	diet	813:816	arg1	3.5SBO					884:889	3.5SBO	884:889	3.5SBO	884:889	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	53	dep	diet	813:816	arg1	2.0SBO					876:881	2.0SBO	876:881	2.0SBO	876:881	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	11	54	theme	CHL	1801:1803	arg1	supplementation					1805:1819	the CHL supplementation	1797:1819	the CHL supplementation	1797:1819	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	6	55	theme	caecal	1027:1032	arg1	analysis					1045:1052	the caecal microbiota analysis	1023:1052	the caecal microbiota analysis	1023:1052	Caecum content was collected for the caecal microbiota analysis through 16S rRNA sequencing.					
33573977	2	56	from	compounds	466:474	arg1	excreta					483:489	the excreta	479:489	the excreta	479:489	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	11	57	theme	digesta	1734:1740	arg1	composition					1698:1708	the composition	1694:1708	the composition of the caecal microbial digesta	1694:1740	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	2	58	theme	caecal	502:507	arg1	microbiota					509:518	the caecal microbiota	498:518	the caecal microbiota in broiler chickens	498:538	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	5	59	theme	odour	965:969	arg1	compounds					971:979	odour compounds	965:979	odour compounds	965:979	Fresh excreta were sampled for the analysis of odour compounds by HPLC.					
33573977	11	60	theme	caecal	1717:1722	arg1	digesta					1734:1740	the caecal microbial digesta	1713:1740	the caecal microbial digesta	1713:1740	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	13	61	theme	strong	2059:2064	arg1	tendency					2066:2073	a strong tendency	2057:2073	a strong tendency to enrich Ruminococcus and to reduce the abundance of Rikenella	2057:2137	By contrast, the CHL supplementation demonstrated a strong tendency to enrich Ruminococcus and to reduce the abundance of Rikenella.					
33573977	12	62	theme	SBO	1840:1842	arg1	supplementation					1844:1858	The SBO supplementation	1836:1858	The SBO supplementation	1836:1858	The SBO supplementation decreased the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta.					
33573977	1	63	from	safety	201:206	arg1	industry					223:230	the poultry industry	211:230	the poultry industry	211:230	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	1	64	theme	emissions	130:138	arg1	Abatement					111:119	Abatement	111:119	Abatement of odour emissions in poultry production	111:160	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	1	65	from	quality	189:195	arg1	industry					223:230	the poultry industry	211:230	the poultry industry	211:230	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	1	66	theme	poultry	143:149	arg1	production					151:160	poultry production	143:160	poultry production	143:160	Abatement of odour emissions in poultry production is important to ensure the quality and safety in the poultry industry as well as for the benefit of the environment.					
33573977	4	67	dep	NC	730:731	arg1	diet					740:743	basal diet	734:743	basal diet	734:743	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	2	68	theme	odour-causing	452:464	arg1	compounds					466:474	the major odour-causing compounds	442:474	the major odour-causing compounds in the excreta	442:489	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	0	69	from	microbiota	87:96	arg1	broilers					101:108	broilers	101:108	broilers	101:108	Soybean oligosaccharides attenuate odour compounds in excreta by modulating the caecal microbiota in broilers.					
33573977	9	70	from	concentrations	1446:1459	arg1	birds					1464:1468	birds	1464:1468	birds fed with 3.5SBO and 5.0SBO	1464:1495	The formate concentrations in birds fed with 3.5SBO and 5.0SBO were higher than those in birds fed with the other diets (P < 0.001).					
33573977	13	71	dep	tendency	2066:2073	arg1	reduce					2105:2110	reduce	2105:2110	to reduce the abundance of Rikenella	2102:2137	By contrast, the CHL supplementation demonstrated a strong tendency to enrich Ruminococcus and to reduce the abundance of Rikenella.					
33573977	13	71	dep	tendency	2066:2073	arg1	enrich					2078:2083	enrich	2078:2083	to enrich Ruminococcus	2075:2096	By contrast, the CHL supplementation demonstrated a strong tendency to enrich Ruminococcus and to reduce the abundance of Rikenella.					
33573977	2	72	theme	dietary	372:378	arg1	SBO					406:408	SBO	406:408	SBO	406:408	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	72	theme	dietary	372:378	arg1	oligosaccharides					388:403	dietary soybean oligosaccharides	372:403	dietary soybean oligosaccharides (SBO)	372:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	11	73	theme	rRNA	1675:1678	arg1	sequencing					1657:1666	Deep sequencing	1652:1666	Deep sequencing of 16S rRNA	1652:1678	Deep sequencing of 16S rRNA revealed that the composition of the caecal microbial digesta was slightly or significantly changed by the SBO or by the CHL supplementation, respectively.					
33573977	0	74	theme	odour	35:39	arg1	compounds					41:49	odour compounds	35:49	odour compounds	35:49	Soybean oligosaccharides attenuate odour compounds in excreta by modulating the caecal microbiota in broilers.					
33573977	4	75	theme	negative	712:719	arg1	NC					730:731	NC	730:731	NC	730:731	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	4	75	theme	negative	712:719	arg1	control					721:727	negative control	712:727	negative control (NC; basal diet)	712:744	The treatments were as follows: negative control (NC; basal diet), positive control (PC; basal diet supplemented with CHL) and basal diet supplemented with 0.5, 2.0, 3.5 and 5.0 g/kg SBO (0.5SBO, 2.0SBO, 3.5SBO and 5.0SBO, respectively).					
33573977	12	76	theme	skatole	1957:1963	arg1	concentrations					1976:1989	the skatole and indole concentrations	1953:1989	the skatole and indole concentrations in the excreta	1953:2004	The SBO supplementation decreased the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta.					
33573977	2	77	theme	oligosaccharides	388:403	arg1	amounts					361:367	different amounts	351:367	different amounts of dietary soybean oligosaccharides (SBO)	351:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	77	theme	oligosaccharides	388:403	arg1	SBO					406:408	SBO	406:408	SBO	406:408	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	2	77	theme	oligosaccharides	388:403	arg1	oligosaccharides					388:403	dietary soybean oligosaccharides	372:403	dietary soybean oligosaccharides (SBO)	372:409	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	14	78	theme	broiler	2338:2344	arg1	chickens					2346:2353	broiler chickens	2338:2353	broiler chickens	2338:2353	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	12	79	theme	indole	1969:1974	arg1	concentrations					1976:1989	the skatole and indole concentrations	1953:1989	the skatole and indole concentrations in the excreta	1953:2004	The SBO supplementation decreased the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta.					
33573977	12	80	from	concentrations	1976:1989	arg1	excreta					1998:2004	the excreta	1994:2004	the excreta	1994:2004	The SBO supplementation decreased the abundance of Bacteroides, Bilophilaand Escherichia, which were associated with the skatole and indole concentrations in the excreta.					
33573977	14	81	theme	microbiota	2324:2333	arg1	composition					2302:2312	the composition	2298:2312	the composition of caecal microbiota in broiler chickens	2298:2353	These results indicated that supplementation with dietary SBO is beneficial in attenuating the concentration of odour-causing compounds and that it modulates the composition of caecal microbiota in broiler chickens.					
33573977	2	82	from	microbiota	509:518	arg1	chickens					531:538	broiler chickens	523:538	broiler chickens	523:538	This study was conducted to compare the effects of supplementation with different amounts of dietary soybean oligosaccharides (SBO) and chlortetracycline (CHL) on the major odour-causing compounds in the excreta and on the caecal microbiota in broiler chickens.					
33573977	9	83	theme	formate	1438:1444	arg1	higher					1502:1507	higher	1502:1507	higher	1502:1507	The formate concentrations in birds fed with 3.5SBO and 5.0SBO were higher than those in birds fed with the other diets (P < 0.001).					
33573977	9	83	theme	formate	1438:1444	arg1	concentrations					1446:1459	The formate concentrations	1434:1459	The formate concentrations in birds fed with 3.5SBO and 5.0SBO	1434:1495	The formate concentrations in birds fed with 3.5SBO and 5.0SBO were higher than those in birds fed with the other diets (P < 0.001).					
33573977	7	84	theme	excreta	1118:1124	arg1	concentration					1133:1145	the excreta indole concentration	1114:1145	the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO	1114:1196	Results showed that on day 42, the excreta indole concentration in the broilers fed with 2.0SBO, 3.5SBO and 5.0SBO and PC was significantly decreased (P < 0.01) compared with that in the NC-fed broilers.					
33573977	3	85	dep	6	636:636	arg1	replicates					638:647	replicates	638:647	replicates	638:647	One-day-old broiler chickens were subjected to a 42-day experiment involving 6 treatments with 6 replicates per treatment (10 birds/cage).					
32283737	8	0	theme	migration	1345:1353	arg1	limit					1355:1359	overall migration limit	1337:1359	overall migration limit	1337:1359	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	8	0	theme	migration	1345:1353	arg1	OML					1332:1334	the OML	1328:1334	the OML (overall migration limit)	1328:1360	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	1	1	theme	chitosan-extract	226:241	arg1	dispersions					256:266	chitosan-extract nanoparticle dispersions	226:266	chitosan-extract nanoparticle dispersions	226:266	Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions.					
32283737	0	2	theme	Colloidal	80:88	arg1	Formulations					90:101	Polyphenol Colloidal Formulations	69:101	Polyphenol Colloidal Formulations	69:101	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.					
32283737	5	3	theme	barrier	648:654	arg1	parameter					704:712	the most important parameter	685:712	the most important parameter influencing antimicrobial and antioxidant activity	685:763	Moreover, barrier properties were examined, as the most important parameter influencing antimicrobial and antioxidant activity.					
32283737	5	3	theme	barrier	648:654	arg1	properties					656:665	barrier properties	648:665	barrier properties	648:665	Moreover, barrier properties were examined, as the most important parameter influencing antimicrobial and antioxidant activity.					
32283737	7	4	theme	great	1065:1069	arg1	properties					1081:1090	great bioactive properties	1065:1090	great bioactive properties	1065:1090	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	1	5	theme	nanoparticle	243:254	arg1	dispersions					256:266	chitosan-extract nanoparticle dispersions	226:266	chitosan-extract nanoparticle dispersions	226:266	Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions.					
32283737	6	6	theme	direct	909:914	arg1	correlation					916:926	any direct correlation	905:926	any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property	905:1044	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	6	7	theme	surface	827:833	arg1	parameters					835:844	different surface parameters	817:844	different surface parameters	817:844	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	2	8	theme	macromolecular	283:296	arg1	chitosan					298:305	macromolecular chitosan	283:305	macromolecular chitosan	283:305	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	9	9	theme	surface	1432:1438	arg1	properties					1440:1449	surface properties	1432:1449	surface properties of foils	1432:1458	Furthermore, surface properties of foils influence to some extent the desired bioactivity.					
32283737	7	10	theme	additive	1119:1126	arg1	effect					1128:1133	the additive effect	1115:1133	the additive effect of antimicrobial chitosan and antioxidative plant extracts	1115:1192	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	6	11	theme	different	817:825	arg1	parameters					835:844	different surface parameters	817:844	different surface parameters	817:844	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	3	12	theme	view	537:540	arg1	point					528:532	a bioactive point	516:532	a bioactive point of view	516:540	Functionalized foils were analyzed from a bioactive point of view, i.e., regarding antimicrobial and antioxidant activity.					
32283737	5	13	theme	antioxidant	744:754	arg1	activity					756:763	antioxidant activity	744:763	antioxidant activity	744:763	Moreover, barrier properties were examined, as the most important parameter influencing antimicrobial and antioxidant activity.					
32283737	2	14	theme	first	335:339	arg1	layer					469:473	an upper layer	460:473	an upper layer	460:473	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	2	14	theme	first	335:339	arg1	layer					341:345	a first layer	333:345	a first layer	333:345	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	8	15	theme	chitosan	1287:1294	arg1	migration					1258:1266	the migration	1254:1266	the migration of polyphenols and chitosan from the foil surface	1254:1316	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	3	16	theme	bioactive	518:526	arg1	point					528:532	a bioactive point	516:532	a bioactive point of view	516:540	Functionalized foils were analyzed from a bioactive point of view, i.e., regarding antimicrobial and antioxidant activity.					
32283737	2	17	theme	various	378:384	arg1	extracts					386:393	various extracts	378:393	various extracts encapsulated into chitosan nanoparticles	378:434	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	9	18	theme	foils	1454:1458	arg1	properties					1440:1449	surface properties	1432:1449	surface properties of foils	1432:1458	Furthermore, surface properties of foils influence to some extent the desired bioactivity.					
32283737	8	19	theme	oxygen	1205:1210	arg1	permeability					1212:1223	oxygen permeability	1205:1223	oxygen permeability	1205:1223	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	0	20	theme	Bioactive	0:8	arg1	Characterization					10:25	Bioactive Characterization	0:25	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.	0:102	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.					
32283737	1	21	theme	Polypropylene	104:116	arg1	foils					145:149	Polypropylene (PP) and polyethylene (PE) foils	104:149	Polypropylene (PP) and polyethylene (PE) foils	104:149	Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions.					
32283737	1	22	theme	ultraviolet	176:186	arg1	/ozone					192:197	ultraviolet (UV)/ozone	176:197	ultraviolet (UV)/ozone	176:197	Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions.					
32283737	0	23	theme	Packaging	30:38	arg1	Foils					40:44	Packaging Foils	30:44	Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations	30:101	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.					
32283737	6	24	theme	contact	983:989	arg1	angle					991:995	contact angle	983:995	contact angle	983:995	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	7	25	theme	bioactive	1071:1079	arg1	properties					1081:1090	great bioactive properties	1065:1090	great bioactive properties	1065:1090	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	8	26	from	surface	1310:1316	arg1	migration					1258:1266	the migration	1254:1266	the migration of polyphenols and chitosan from the foil surface	1254:1316	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	8	27	theme	overall	1337:1343	arg1	limit					1355:1359	overall migration limit	1337:1359	overall migration limit	1337:1359	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	8	27	theme	overall	1337:1343	arg1	OML					1332:1334	the OML	1328:1334	the OML (overall migration limit)	1328:1360	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	8	28	theme	industry	1396:1403	arg1	applications					1405:1416	food industry applications	1391:1416	food industry applications	1391:1416	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	2	29	theme	chitosan	413:420	arg1	nanoparticles					422:434	chitosan nanoparticles	413:434	chitosan nanoparticles	413:434	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	7	30	theme	extracts	1185:1192	arg1	effect					1128:1133	the additive effect	1115:1133	the additive effect of antimicrobial chitosan and antioxidative plant extracts	1115:1192	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	5	31	theme	important	694:702	arg1	properties					656:665	barrier properties	648:665	barrier properties	648:665	Moreover, barrier properties were examined, as the most important parameter influencing antimicrobial and antioxidant activity.					
32283737	5	31	theme	important	694:702	arg1	parameter					704:712	the most important parameter	685:712	the most important parameter influencing antimicrobial and antioxidant activity	685:763	Moreover, barrier properties were examined, as the most important parameter influencing antimicrobial and antioxidant activity.					
32283737	2	32	theme	chitosan	298:305	arg1	solution					271:278	A solution	269:278	A solution of macromolecular chitosan	269:305	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	1	33	theme	UV	189:190	arg1	/ozone					192:197	ultraviolet (UV)/ozone	176:197	ultraviolet (UV)/ozone	176:197	Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions.					
32283737	2	34	theme	upper	463:467	arg1	layer					469:473	an upper layer	460:473	an upper layer	460:473	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	2	34	theme	upper	463:467	arg1	layer					341:345	a first layer	333:345	a first layer	333:345	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	3	35	theme	antioxidant	577:587	arg1	activity					589:596	antioxidant activity	577:596	antioxidant activity	577:596	Functionalized foils were analyzed from a bioactive point of view, i.e., regarding antimicrobial and antioxidant activity.					
32283737	8	36	theme	polyphenols	1271:1281	arg1	migration					1258:1266	the migration	1254:1266	the migration of polyphenols and chitosan from the foil surface	1254:1316	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	8	37	theme	foil	1305:1308	arg1	surface					1310:1316	the foil surface	1301:1316	the foil surface	1301:1316	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	0	38	theme	Foils	40:44	arg1	Characterization					10:25	Bioactive Characterization	0:25	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.	0:102	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.					
32283737	6	39	theme	surface	967:973	arg1	charge					975:980	surface charge	967:980	surface charge	967:980	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	7	40	theme	antioxidative	1165:1177	arg1	extracts					1185:1192	antioxidative plant extracts	1165:1192	antioxidative plant extracts	1165:1192	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	7	41	theme	plant	1179:1183	arg1	extracts					1185:1192	antioxidative plant extracts	1165:1192	antioxidative plant extracts	1165:1192	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	2	42	theme	extracts	386:393	arg1	deposition					364:373	the deposition	360:373	the deposition of various extracts encapsulated into chitosan nanoparticles	360:434	A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer.					
32283737	7	43	theme	antimicrobial	1138:1150	arg1	chitosan					1152:1159	antimicrobial chitosan	1138:1159	antimicrobial chitosan	1138:1159	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	3	44	theme	Functionalized	476:489	arg1	foils					491:495	Functionalized foils	476:495	Functionalized foils	476:495	Functionalized foils were analyzed from a bioactive point of view, i.e., regarding antimicrobial and antioxidant activity.					
32283737	7	45	theme	chitosan	1152:1159	arg1	effect					1128:1133	the additive effect	1115:1133	the additive effect of antimicrobial chitosan and antioxidative plant extracts	1115:1192	It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts.					
32283737	6	46	theme	elemental	944:952	arg1	composition					954:964	surface elemental composition	936:964	surface elemental composition	936:964	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	8	47	theme	food	1391:1394	arg1	applications					1405:1416	food industry applications	1391:1416	food industry applications	1391:1416	Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications.					
32283737	1	48	theme	polyethylene	127:138	arg1	foils					145:149	Polypropylene (PP) and polyethylene (PE) foils	104:149	Polypropylene (PP) and polyethylene (PE) foils	104:149	Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions.					
32283737	4	49	theme	Desorption	599:608	arg1	kinetics					610:617	Desorption kinetics	599:617	Desorption kinetics	599:617	Desorption kinetics were also studied.					
32283737	6	50	theme	bioactive	1027:1035	arg1	property					1037:1044	a specific bioactive property	1016:1044	a specific bioactive property	1016:1044	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	0	51	theme	Polyphenol	69:78	arg1	Formulations					90:101	Polyphenol Colloidal Formulations	69:101	Polyphenol Colloidal Formulations	69:101	Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations.					
32283737	9	52	theme	desired	1489:1495	arg1	bioactivity					1497:1507	the desired bioactivity	1485:1507	the desired bioactivity	1485:1507	Furthermore, surface properties of foils influence to some extent the desired bioactivity.					
32283737	6	53	theme	specific	1018:1025	arg1	property					1037:1044	a specific bioactive property	1016:1044	a specific bioactive property	1016:1044	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
32283737	6	54	theme	surface	936:942	arg1	composition					954:964	surface elemental composition	936:964	surface elemental composition	936:964	Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property.					
31880918	6	0	theme	heterogeneous	1185:1197	arg1	catalysts					1199:1207	heterogeneous catalysts	1185:1207	heterogeneous catalysts for organic reactions	1185:1229	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	8	1	theme	other	1455:1459	arg1	materials					1477:1485	other hybrid chitosan materials	1455:1485	other hybrid chitosan materials	1455:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	8	2	theme	materials	1477:1485	arg1	materials					1477:1485	other hybrid chitosan materials	1455:1485	other hybrid chitosan materials	1455:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	8	2	theme	materials	1477:1485	arg1	variety					1444:1450	a wide variety	1437:1450	a wide variety of other hybrid chitosan materials	1437:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	3	3	theme	controlled	496:505	arg1	sizes					507:511	controlled sizes	496:511	controlled sizes	496:511	Interestingly, when the hydrogel is prepared in the presence of HAuCl4, GNPs with controlled sizes between 15 and 50 nm are obtained, which are homogeneously distributed within the plasmonic hydrogels (GNPs-CS-Sq).					
31880918	8	4	theme	chitosan	1468:1475	arg1	materials					1477:1485	other hybrid chitosan materials	1455:1485	other hybrid chitosan materials	1455:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	5	5	theme	bioinspired	924:934	arg1	reactor					936:942	a bioinspired reactor	922:942	a bioinspired reactor	922:942	Accordingly, the hydrogel acts as a bioinspired reactor and permits to gain certain control on the size of GNPs by adjusting the concentration of chitosan and HAuCl4.					
31880918	5	5	theme	bioinspired	924:934	arg1	hydrogel					905:912	the hydrogel	901:912	the hydrogel	901:912	Accordingly, the hydrogel acts as a bioinspired reactor and permits to gain certain control on the size of GNPs by adjusting the concentration of chitosan and HAuCl4.					
31880918	6	6	theme	GNPs-CS-Sq	1117:1126	arg1	hydrogels					1128:1136	the GNPs-CS-Sq hydrogels	1113:1136	the GNPs-CS-Sq hydrogels	1113:1136	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	5	7	theme	HAuCl4	1047:1052	arg1	concentration					1017:1029	the concentration	1013:1029	the concentration of chitosan and HAuCl4	1013:1052	Accordingly, the hydrogel acts as a bioinspired reactor and permits to gain certain control on the size of GNPs by adjusting the concentration of chitosan and HAuCl4.					
31880918	3	8	dep	HAuCl4	478:483	arg1	the					462:464	the	462:464	the	462:464	Interestingly, when the hydrogel is prepared in the presence of HAuCl4, GNPs with controlled sizes between 15 and 50 nm are obtained, which are homogeneously distributed within the plasmonic hydrogels (GNPs-CS-Sq).					
31880918	3	8	dep	HAuCl4	478:483	arg1	presence					466:473	presence	466:473	presence	466:473	Interestingly, when the hydrogel is prepared in the presence of HAuCl4, GNPs with controlled sizes between 15 and 50 nm are obtained, which are homogeneously distributed within the plasmonic hydrogels (GNPs-CS-Sq).					
31880918	4	9	theme	growth	728:733	arg1	process					735:741	the growth process	724:741	the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs	724:885	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	6	10	theme	intrinsic	1067:1075	arg1	properties					1099:1108	the intrinsic and tunable plasmonic properties	1063:1108	the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels	1063:1136	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	8	11	theme	useful	1487:1492	arg1	applications					1573:1584	the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications	1400:1584	the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications	1400:1584	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	6	12	theme	organic	1213:1219	arg1	reactions					1221:1229	organic reactions	1213:1229	organic reactions	1213:1229	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	4	13	theme	GNPs	746:749	arg1	process					735:741	the growth process	724:741	the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs	724:885	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	14	theme	supramolecular	647:660	arg1	nature					662:667	the supramolecular nature	643:667	the supramolecular nature	643:667	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	14	theme	supramolecular	647:660	arg1	key					716:718	key	716:718	key	716:718	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	7	15	theme	new	1286:1288	arg1	hydrogels					1290:1298	the new hydrogels	1282:1298	the new hydrogels	1282:1298	Furthermore, cell viability studies indicate that the new hydrogels exhibit suitable biocompatibility.					
31880918	6	16	theme	hydrogels	1128:1136	arg1	properties					1099:1108	the intrinsic and tunable plasmonic properties	1063:1108	the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels	1063:1136	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	8	17	theme	cell	1521:1524	arg1	culture					1526:1532	cell culture	1521:1532	cell culture	1521:1532	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	4	18	theme	hydrogel	821:828	arg1	network					830:836	the chitosan hydrogel network	808:836	the chitosan hydrogel network	808:836	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	2	19	theme	noncovalent	310:320	arg1	interactions					322:333	noncovalent interactions	310:333	noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan	310:411	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
31880918	2	20	theme	acid	383:386	arg1	derivatives					388:398	the in situ formed ionic squaric acid derivatives	350:398	the in situ formed ionic squaric acid derivatives	350:398	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
31880918	3	21	with	GNPs	486:489	arg1	sizes					507:511	controlled sizes	496:511	controlled sizes	496:511	Interestingly, when the hydrogel is prepared in the presence of HAuCl4, GNPs with controlled sizes between 15 and 50 nm are obtained, which are homogeneously distributed within the plasmonic hydrogels (GNPs-CS-Sq).					
31880918	8	22	theme	wide	1439:1442	arg1	materials					1477:1485	other hybrid chitosan materials	1455:1485	other hybrid chitosan materials	1455:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	8	22	theme	wide	1439:1442	arg1	variety					1444:1450	a wide variety	1437:1450	a wide variety of other hybrid chitosan materials	1437:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	2	23	theme	squaric	375:381	arg1	derivatives					388:398	the in situ formed ionic squaric acid derivatives	350:398	the in situ formed ionic squaric acid derivatives	350:398	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
31880918	0	24	theme	Chitosan-Squarate	25:41	arg1	Development					0:10	Development	0:10	Development of Plasmonic Chitosan-Squarate	0:41	Development of Plasmonic Chitosan-Squarate Hydrogels via Bioinspired Nanoparticle Growth.					
31880918	8	25	theme	potential	1404:1412	arg1	applications					1573:1584	the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications	1400:1584	the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications	1400:1584	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	4	26	theme	reducing	788:795	arg1	GNPs					882:885	the GNPs	878:885	the GNPs	878:885	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	26	theme	reducing	788:795	arg1	points					858:863	nucleation points	847:863	nucleation points	847:863	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	26	theme	reducing	788:795	arg1	derivatives					769:779	the squaric derivatives	757:779	the squaric derivatives	757:779	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	26	theme	reducing	788:795	arg1	agents					797:802	reducing agents	788:802	reducing agents	788:802	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	1	27	theme	bioinspired	107:117	arg1	growth					119:124	the bioinspired growth	103:124	the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels	103:180	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	1	28	theme	hybrid	203:208	arg1	materials					210:218	plasmonic hybrid materials	193:218	plasmonic hybrid materials	193:218	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	0	29	theme	Plasmonic	15:23	arg1	Chitosan-Squarate					25:41	Plasmonic Chitosan-Squarate	15:41	Plasmonic Chitosan-Squarate	15:41	Development of Plasmonic Chitosan-Squarate Hydrogels via Bioinspired Nanoparticle Growth.					
31880918	1	30	theme	biocompatible	158:170	arg1	hydrogels					172:180	biocompatible hydrogels	158:180	biocompatible hydrogels	158:180	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	8	31	contain	has	1396:1398	arg1	method					1354:1359	the proposed method	1341:1359	the proposed method for obtaining GNPs-CS-Sq hydrogels	1341:1394	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	8	31	contain	has	1396:1398	arg2	applications					1573:1584	the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications	1400:1584	the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications	1400:1584	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	6	32	theme	tunable	1081:1087	arg1	properties					1099:1108	the intrinsic and tunable plasmonic properties	1063:1108	the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels	1063:1136	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	1	33	theme	plasmonic	193:201	arg1	materials					210:218	plasmonic hybrid materials	193:218	plasmonic hybrid materials	193:218	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	7	34	theme	cell	1245:1248	arg1	studies					1260:1266	cell viability studies	1245:1266	cell viability studies	1245:1266	Furthermore, cell viability studies indicate that the new hydrogels exhibit suitable biocompatibility.					
31880918	8	35	theme	tissue	1535:1540	arg1	engineering					1542:1552	tissue engineering	1535:1552	tissue engineering	1535:1552	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	5	36	theme	chitosan	1034:1041	arg1	concentration					1017:1029	the concentration	1013:1029	the concentration of chitosan and HAuCl4	1013:1052	Accordingly, the hydrogel acts as a bioinspired reactor and permits to gain certain control on the size of GNPs by adjusting the concentration of chitosan and HAuCl4.					
31880918	7	37	theme	viability	1250:1258	arg1	studies					1260:1266	cell viability studies	1245:1266	cell viability studies	1245:1266	Furthermore, cell viability studies indicate that the new hydrogels exhibit suitable biocompatibility.					
31880918	0	38	theme	Nanoparticle	69:80	arg1	Growth					82:87	Bioinspired Nanoparticle Growth	57:87	Bioinspired Nanoparticle Growth	57:87	Development of Plasmonic Chitosan-Squarate Hydrogels via Bioinspired Nanoparticle Growth.					
31880918	8	39	theme	GNPs-CS-Sq	1375:1384	arg1	hydrogels					1386:1394	GNPs-CS-Sq hydrogels	1375:1394	GNPs-CS-Sq hydrogels	1375:1394	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	4	40	theme	chitosan	812:819	arg1	network					830:836	the chitosan hydrogel network	808:836	the chitosan hydrogel network	808:836	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	6	41	theme	plasmonic	1089:1097	arg1	properties					1099:1108	the intrinsic and tunable plasmonic properties	1063:1108	the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels	1063:1136	Besides the intrinsic and tunable plasmonic properties of the GNPs-CS-Sq hydrogels, it was found that the gels could be useful as heterogeneous catalysts for organic reactions.					
31880918	0	42	theme	Bioinspired	57:67	arg1	Growth					82:87	Bioinspired Nanoparticle Growth	57:87	Bioinspired Nanoparticle Growth	57:87	Development of Plasmonic Chitosan-Squarate Hydrogels via Bioinspired Nanoparticle Growth.					
31880918	5	43	theme	GNPs	995:998	arg1	size					987:990	the size	983:990	the size of GNPs	983:998	Accordingly, the hydrogel acts as a bioinspired reactor and permits to gain certain control on the size of GNPs by adjusting the concentration of chitosan and HAuCl4.					
31880918	2	44	theme	new	225:227	arg1	CS-Sq					239:243	CS-Sq	239:243	CS-Sq	239:243	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
31880918	2	44	theme	new	225:227	arg1	hydrogel					229:236	The new hydrogel	221:236	The new hydrogel (CS-Sq)	221:244	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
31880918	1	45	theme	gold	129:132	arg1	nanoparticles					134:146	gold nanoparticles	129:146	gold nanoparticles (GNPs)	129:153	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	1	45	theme	gold	129:132	arg1	GNPs					149:152	GNPs	149:152	GNPs	149:152	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	4	46	theme	squaric	761:767	arg1	GNPs					882:885	the GNPs	878:885	the GNPs	878:885	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	46	theme	squaric	761:767	arg1	derivatives					769:779	the squaric derivatives	757:779	the squaric derivatives	757:779	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	46	theme	squaric	761:767	arg1	points					858:863	nucleation points	847:863	nucleation points	847:863	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	46	theme	squaric	761:767	arg1	agents					797:802	reducing agents	788:802	reducing agents	788:802	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	7	47	theme	suitable	1308:1315	arg1	biocompatibility					1317:1332	suitable biocompatibility	1308:1332	suitable biocompatibility	1308:1332	Furthermore, cell viability studies indicate that the new hydrogels exhibit suitable biocompatibility.					
31880918	1	48	theme	nanoparticles	134:146	arg1	growth					119:124	the bioinspired growth	103:124	the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels	103:180	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	1	49	from	growth	119:124	arg1	hydrogels					172:180	biocompatible hydrogels	158:180	biocompatible hydrogels	158:180	We report on the bioinspired growth of gold nanoparticles (GNPs) in biocompatible hydrogels to develop plasmonic hybrid materials.					
31880918	8	50	theme	drug	1559:1562	arg1	delivery					1564:1571	drug delivery	1559:1571	drug delivery	1559:1571	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	4	51	theme	CS-Sq	696:700	arg1	hydrogels					702:710	the CS-Sq hydrogels	692:710	the CS-Sq hydrogels	692:710	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	52	theme	nucleation	847:856	arg1	GNPs					882:885	the GNPs	878:885	the GNPs	878:885	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	52	theme	nucleation	847:856	arg1	agents					797:802	reducing agents	788:802	reducing agents	788:802	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	52	theme	nucleation	847:856	arg1	derivatives					769:779	the squaric derivatives	757:779	the squaric derivatives	757:779	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	52	theme	nucleation	847:856	arg1	points					858:863	nucleation points	847:863	nucleation points	847:863	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	53	theme	hydrogels	702:710	arg1	nature					662:667	the supramolecular nature	643:667	the supramolecular nature	643:667	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	53	theme	hydrogels	702:710	arg1	composition					677:687	the composition	673:687	the composition of the CS-Sq hydrogels	673:710	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	4	53	theme	hydrogels	702:710	arg1	key					716:718	key	716:718	key	716:718	We found that the supramolecular nature and the composition of the CS-Sq hydrogels are key for the growth process of GNPs where the squaric derivatives act as reducing agents and the chitosan hydrogel network provides nucleation points and supports the GNPs.					
31880918	8	54	theme	variety	1444:1450	arg1	development					1422:1432	the development	1418:1432	the development of a wide variety of other hybrid chitosan materials	1418:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	5	55	theme	certain	964:970	arg1	control					972:978	certain control	964:978	certain control	964:978	Accordingly, the hydrogel acts as a bioinspired reactor and permits to gain certain control on the size of GNPs by adjusting the concentration of chitosan and HAuCl4.					
31880918	2	56	theme	ionic	369:373	arg1	derivatives					388:398	the in situ formed ionic squaric acid derivatives	350:398	the in situ formed ionic squaric acid derivatives	350:398	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
31880918	8	57	theme	proposed	1345:1352	arg1	method					1354:1359	the proposed method	1341:1359	the proposed method for obtaining GNPs-CS-Sq hydrogels	1341:1394	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	8	58	theme	hybrid	1461:1466	arg1	materials					1477:1485	other hybrid chitosan materials	1455:1485	other hybrid chitosan materials	1455:1485	Thus, the proposed method for obtaining GNPs-CS-Sq hydrogels has the potential for the development of a wide variety of other hybrid chitosan materials useful for catalysis, biosensing, cell culture, tissue engineering, and drug delivery applications.					
31880918	3	59	theme	plasmonic	595:603	arg1	GNPs-CS-Sq					616:625	GNPs-CS-Sq	616:625	GNPs-CS-Sq	616:625	Interestingly, when the hydrogel is prepared in the presence of HAuCl4, GNPs with controlled sizes between 15 and 50 nm are obtained, which are homogeneously distributed within the plasmonic hydrogels (GNPs-CS-Sq).					
31880918	3	59	theme	plasmonic	595:603	arg1	hydrogels					605:613	the plasmonic hydrogels	591:613	the plasmonic hydrogels (GNPs-CS-Sq)	591:626	Interestingly, when the hydrogel is prepared in the presence of HAuCl4, GNPs with controlled sizes between 15 and 50 nm are obtained, which are homogeneously distributed within the plasmonic hydrogels (GNPs-CS-Sq).					
31880918	2	60	theme	formed	362:367	arg1	derivatives					388:398	the in situ formed ionic squaric acid derivatives	350:398	the in situ formed ionic squaric acid derivatives	350:398	The new hydrogel (CS-Sq) is prepared from chitosan and diethylsquarate and is formed via noncovalent interactions rising between the in situ formed ionic squaric acid derivatives and chitosan.					
33599092	0	0	from	changes	14:20	arg1	progression					84:94	colorectal cancer progression	66:94	colorectal cancer progression	66:94	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	3	1	with	patients	508:515	arg1	controls					577:584	50 age- and sex-matched healthy controls	545:584	50 age- and sex-matched healthy controls	545:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	1	2	theme	IgG	172:174	arg1	subclass					176:183	different IgG subclass	162:183	different IgG subclass	162:183	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	3	3	from	patients	531:538	arg1	samples					485:491	EXPERIMENTAL DESIGN Serum samples	459:491	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls	459:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	7	4	theme	biantennary	1219:1229	arg1	glycan					1243:1248	the biantennary fucosylated glycan	1215:1248	the biantennary fucosylated glycan	1215:1248	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	3	5	from	patients	508:515	arg1	samples					485:491	EXPERIMENTAL DESIGN Serum samples	459:491	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls	459:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	2	6	theme	glycosylation	358:370	arg1	changes					325:331	the changes	321:331	the changes of subclass-specific IgG glycosylation in CRC	321:377	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	5	7	theme	MS	768:769	arg1	data					771:774	The exported MS data	755:774	The exported MS data	755:774	The exported MS data was automatically and rapidly processed by the self-developed MATLAB code.					
33599092	6	8	theme	IgG2	987:990	arg1	agalactosylation					959:974	remarkably increased agalactosylation	938:974	remarkably increased agalactosylation	938:974	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	6	8	theme	IgG2	987:990	arg1	galactosylation					918:932	significantly decreased galactosylation	894:932	significantly decreased galactosylation	894:932	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	2	9	from	changes	325:331	arg1	CRC					375:377	CRC	375:377	CRC	375:377	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	2	10	theme	analytical	292:301	arg1	method					303:308	a simple and high-throughput analytical method	263:308	a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease	263:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	3	11	theme	sex-matched	557:567	arg1	controls					577:584	50 age- and sex-matched healthy controls	545:584	50 age- and sex-matched healthy controls	545:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	6	12	theme	cancer	1074:1079	arg1	patients					1081:1088	cancer patients	1074:1088	cancer patients	1074:1088	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	0	13	theme	high-throughput	99:113	arg1	assay					115:119	high-throughput assay	99:119	high-throughput assay	99:119	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	3	14	theme	age-	548:551	arg1	controls					577:584	50 age- and sex-matched healthy controls	545:584	50 age- and sex-matched healthy controls	545:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	7	15	theme	IgG2	1320:1323	arg1	galactose					1289:1297	two galactose	1285:1297	two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups	1285:1390	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	7	15	theme	IgG2	1320:1323	arg1	H5N4F1					1300:1305	H5N4F1	1300:1305	H5N4F1	1300:1305	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	2	16	theme	high-throughput	276:290	arg1	method					303:308	a simple and high-throughput analytical method	263:308	a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease	263:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	6	17	theme	IgG1	979:982	arg1	agalactosylation					959:974	remarkably increased agalactosylation	938:974	remarkably increased agalactosylation	938:974	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	6	17	theme	IgG1	979:982	arg1	galactosylation					918:932	significantly decreased galactosylation	894:932	significantly decreased galactosylation	894:932	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	5	18	dep	MATLAB	838:843	arg1	code					845:848	code	845:848	code	845:848	The exported MS data was automatically and rapidly processed by the self-developed MATLAB code.					
33599092	4	19	theme	IgG	649:651	arg1	glycosylation					653:665	Subclass-specific IgG glycosylation	631:665	Subclass-specific IgG glycosylation	631:665	Subclass-specific IgG glycosylation was profiled by MALDI-MS followed by the structural identification through MALDI-MS/MS.					
33599092	8	20	theme	simple	1440:1445	arg1	procedures					1467:1476	the simple and high-throughput procedures	1436:1476	the simple and high-throughput procedures	1436:1476	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	7	21	gly	glycopeptides	1192:1204	arg2	glycopeptides					1192:1204	individual glycopeptides	1181:1204	individual glycopeptides	1181:1204	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	3	22	with	patients	531:538	arg1	controls					577:584	50 age- and sex-matched healthy controls	545:584	50 age- and sex-matched healthy controls	545:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	2	23	theme	simple	265:270	arg1	method					303:308	a simple and high-throughput analytical method	263:308	a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease	263:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	1	24	theme	colorectal	188:197	arg1	cancer					199:204	colorectal cancer	188:204	colorectal cancer (CRC)	188:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	1	24	theme	colorectal	188:197	arg1	CRC					207:209	CRC	207:209	CRC	207:209	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	2	25	theme	disease	450:456	arg1	detection					432:440	early detection	426:440	early detection of this disease	426:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	3	26	theme	benign	524:529	arg1	patients					531:538	22 benign patients	521:538	22 benign patients	521:538	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	1	27	from	glycosylation	145:157	arg1	cancer					199:204	colorectal cancer	188:204	colorectal cancer (CRC)	188:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	1	27	from	glycosylation	145:157	arg1	CRC					207:209	CRC	207:209	CRC	207:209	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	3	28	theme	healthy	569:575	arg1	controls					577:584	50 age- and sex-matched healthy controls	545:584	50 age- and sex-matched healthy controls	545:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	6	29	from	galactosylation	918:932	arg1	transformation					1009:1022	the malignant transformation	995:1022	the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls	995:1109	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	8	30	theme	CRC	1569:1571	arg1	pathology					1556:1564	the premature pathology	1542:1564	the premature pathology of CRC	1542:1571	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	7	31	theme	individual	1181:1190	arg1	glycopeptides					1192:1204	individual glycopeptides	1181:1204	individual glycopeptides	1181:1204	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	6	32	dep	RESULTS	851:857	arg1	suggested					880:888	suggested	880:888	suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls	880:1109	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	8	33	theme	IgG	1521:1523	arg1	glycopeptides					1525:1537	IgG glycopeptides	1521:1537	IgG glycopeptides	1521:1537	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	7	34	gly	fucosylated	1231:1241	arg1	glycan					1243:1248	the biantennary fucosylated glycan	1215:1248	the biantennary fucosylated glycan	1215:1248	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	7	35	theme	glycopeptides	1192:1204	arg1	exploration					1166:1176	the exploration	1162:1176	the exploration of individual glycopeptides	1162:1204	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	2	36	theme	early	426:430	arg1	detection					432:440	early detection	426:440	early detection of this disease	426:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	8	37	theme	high-throughput	1451:1465	arg1	procedures					1467:1476	the simple and high-throughput procedures	1436:1476	the simple and high-throughput procedures	1436:1476	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	6	38	theme	increased	949:957	arg1	agalactosylation					959:974	remarkably increased agalactosylation	938:974	remarkably increased agalactosylation	938:974	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	1	39	theme	subclass	176:183	arg1	glycosylation					145:157	glycosylation	145:157	glycosylation of different IgG subclass in colorectal cancer (CRC)	145:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	8	40	from	role	1513:1516	arg1	pathology					1556:1564	the premature pathology	1542:1564	the premature pathology of CRC	1542:1571	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	3	41	theme	Serum	479:483	arg1	samples					485:491	EXPERIMENTAL DESIGN Serum samples	459:491	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls	459:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	0	42	theme	subclass-specific	29:45	arg1	N-glycosylation					47:61	IgG subclass-specific N-glycosylation	25:61	IgG subclass-specific N-glycosylation	25:61	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	2	43	dep	method	303:308	arg1	find					387:390	find	387:390	to find the specific glyco-biomarkers for early detection of this disease	384:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	2	43	dep	method	303:308	arg1	explore					313:319	explore	313:319	to explore the changes of subclass-specific IgG glycosylation in CRC	310:377	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	1	44	gly	glycosylation	145:157	arg1	cancer					199:204	colorectal cancer	188:204	colorectal cancer (CRC)	188:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	1	44	gly	glycosylation	145:157	arg1	CRC					207:209	CRC	207:209	CRC	207:209	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	1	44	gly	glycosylation	145:157	arg1	subclass					176:183	different IgG subclass	162:183	different IgG subclass	162:183	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	8	45	theme	glycopeptides	1525:1537	arg1	role					1513:1516	the important role	1499:1516	the important role of IgG glycopeptides in the premature pathology of CRC	1499:1571	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	5	46	theme	exported	759:766	arg1	data					771:774	The exported MS data	755:774	The exported MS data	755:774	The exported MS data was automatically and rapidly processed by the self-developed MATLAB code.					
33599092	6	47	from	agalactosylation	959:974	arg1	transformation					1009:1022	the malignant transformation	995:1022	the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls	995:1109	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	0	48	theme	IgG	25:27	arg1	N-glycosylation					47:61	IgG subclass-specific N-glycosylation	25:61	IgG subclass-specific N-glycosylation	25:61	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	5	49	theme	self-developed	823:836	arg1	MATLAB					838:843	the self-developed MATLAB	819:843	the self-developed MATLAB code	819:848	The exported MS data was automatically and rapidly processed by the self-developed MATLAB code.					
33599092	8	50	theme	premature	1546:1554	arg1	pathology					1556:1564	the premature pathology	1542:1564	the premature pathology of CRC	1542:1571	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	2	51	theme	specific	396:403	arg1	glyco-biomarkers					405:420	the specific glyco-biomarkers	392:420	the specific glyco-biomarkers for early detection of this disease	392:456	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	7	52	theme	fucosylated	1231:1241	arg1	glycan					1243:1248	the biantennary fucosylated glycan	1215:1248	the biantennary fucosylated glycan	1215:1248	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	6	53	theme	CRC	1027:1029	arg1	transformation					1009:1022	the malignant transformation	995:1022	the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls	995:1109	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	7	54	theme	glycan	1127:1132	arg1	features					1134:1141	glycan features	1127:1141	glycan features	1127:1141	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	7	55	theme	IgG1	1311:1314	arg1	galactose					1289:1297	two galactose	1285:1297	two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups	1285:1390	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	7	55	theme	IgG1	1311:1314	arg1	H5N4F1					1300:1305	H5N4F1	1300:1305	H5N4F1	1300:1305	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	2	56	theme	IgG	354:356	arg1	glycosylation					358:370	subclass-specific IgG glycosylation	336:370	subclass-specific IgG glycosylation	336:370	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	0	57	theme	N-glycosylation	47:61	arg1	changes					14:20	the changes	10:20	the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression	10:94	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	7	58	theme	features	1134:1141	arg1	changes					1116:1122	The changes	1112:1122	The changes of glycan features	1112:1141	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	6	59	theme	decreased	908:916	arg1	galactosylation					918:932	significantly decreased galactosylation	894:932	significantly decreased galactosylation	894:932	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	1	60	theme	different	162:170	arg1	subclass					176:183	different IgG subclass	162:183	different IgG subclass	162:183	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	2	61	theme	subclass-specific	336:352	arg1	glycosylation					358:370	subclass-specific IgG glycosylation	336:370	subclass-specific IgG glycosylation	336:370	The authors aimed to use a simple and high-throughput analytical method to explore the changes of subclass-specific IgG glycosylation in CRC, and to find the specific glyco-biomarkers for early detection of this disease.					
33599092	6	62	dep	galactosylation	918:932	arg1	the					890:892	the	890:892	the	890:892	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	7	63	dep	IgG1	1311:1314	arg1	distinguish					1331:1341	distinguish	1331:1341	could distinguish cancer group from both benign and control groups	1325:1390	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	7	64	dep	benign	1366:1371	arg1	groups					1385:1390	groups	1385:1390	groups	1385:1390	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	6	65	theme	Statistical	859:869	arg1	analysis					871:878	Statistical analysis	859:878	Statistical analysis	859:878	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	7	66	theme	cancer	1343:1348	arg1	group					1350:1354	cancer group	1343:1354	cancer group	1343:1354	The changes of glycan features were elucidated by the exploration of individual glycopeptides, showing the biantennary fucosylated glycan without galactose (H3N4F1) or with two galactose (H5N4F1) of IgG1 and IgG2 could distinguish cancer group from both benign and control groups.					
33599092	1	67	from	PURPOSE	122:128	arg1	cancer					199:204	colorectal cancer	188:204	colorectal cancer (CRC)	188:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	1	67	from	PURPOSE	122:128	arg1	CRC					207:209	CRC	207:209	CRC	207:209	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	8	68	gly	glycopeptides	1525:1537	arg2	glycopeptides					1525:1537	IgG glycopeptides	1521:1537	IgG glycopeptides	1521:1537	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	3	69	theme	50	545:546	arg1	age-					548:551	age-	548:551	age-	548:551	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	1	70	from	cancer	199:204	arg1	PURPOSE					122:128	PURPOSE	122:128	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC)	122:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	1	71	theme	glycosylation	145:157	arg1	PURPOSE					122:128	PURPOSE	122:128	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC)	122:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	8	72	theme	CLINICAL	1409:1416	arg1	RELEVANCE					1418:1426	CLINICAL RELEVANCE	1409:1426	CLINICAL RELEVANCE Through the simple and high-throughput procedures	1409:1476	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	0	73	theme	cancer	77:82	arg1	progression					84:94	colorectal cancer progression	66:94	colorectal cancer progression	66:94	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	3	74	theme	EXPERIMENTAL	459:470	arg1	samples					485:491	EXPERIMENTAL DESIGN Serum samples	459:491	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls	459:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	3	75	theme	independent	610:620	arg1	cohorts					622:628	two independent cohorts	606:628	two independent cohorts	606:628	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	3	76	theme	DESIGN	472:477	arg1	samples					485:491	EXPERIMENTAL DESIGN Serum samples	459:491	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls	459:584	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	4	77	theme	structural	708:717	arg1	identification					719:732	the structural identification	704:732	the structural identification	704:732	Subclass-specific IgG glycosylation was profiled by MALDI-MS followed by the structural identification through MALDI-MS/MS.					
33599092	4	78	theme	Subclass-specific	631:647	arg1	glycosylation					653:665	Subclass-specific IgG glycosylation	631:665	Subclass-specific IgG glycosylation	631:665	Subclass-specific IgG glycosylation was profiled by MALDI-MS followed by the structural identification through MALDI-MS/MS.					
33599092	0	79	theme	colorectal	66:75	arg1	progression					84:94	colorectal cancer progression	66:94	colorectal cancer progression	66:94	Revealing the changes of IgG subclass-specific N-glycosylation in colorectal cancer progression by high-throughput assay.					
33599092	6	80	theme	malignant	999:1007	arg1	transformation					1009:1022	the malignant transformation	995:1022	the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls	995:1109	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
33599092	8	81	theme	important	1503:1511	arg1	role					1513:1516	the important role	1499:1516	the important role of IgG glycopeptides in the premature pathology of CRC	1499:1571	CONCLUSIONS AND CLINICAL RELEVANCE Through the simple and high-throughput procedures, this study revealed the important role of IgG glycopeptides in the premature pathology of CRC.					
33599092	1	82	dep	PURPOSE	122:128	arg1	changes					134:140	The changes	130:140	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC)	122:210	PURPOSE The changes of glycosylation of different IgG subclass in colorectal cancer (CRC) were rarely investigated.					
33599092	3	83	theme	cancer	501:506	arg1	patients					508:515	71 cancer patients	498:515	71 cancer patients	498:515	EXPERIMENTAL DESIGN Serum samples from 71 cancer patients and 22 benign patients with 50 age- and sex-matched healthy controls were collected from two independent cohorts.					
33599092	6	84	theme	healthy	1094:1100	arg1	controls					1102:1109	healthy controls	1094:1109	healthy controls	1094:1109	RESULTS Statistical analysis suggested the significantly decreased galactosylation and remarkably increased agalactosylation of IgG1 or IgG2 in the malignant transformation of CRC, which enables the differentiation between cancer patients and healthy controls.					
32829853	1	0	theme	TBBPA	334:338	arg1	degradation					340:350	TBBPA degradation	334:350	TBBPA degradation	334:350	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	7	1	theme	optimal	1025:1031	arg1	conditions					1033:1042	optimal conditions	1025:1042	optimal conditions	1025:1042	Almost 100 % of TBBPA was degraded within 30 min under optimal conditions, and the rate constant was determined to be 0.764 min-1.					
32829853	8	2	theme	degradation	1118:1128	arg1	%					1168:1168	60 %	1165:1168	60 %	1165:1168	Furthermore, the degradation rate of the composite membrane was 60 % after 4 cycles.					
32829853	8	2	theme	degradation	1118:1128	arg1	rate					1130:1133	the degradation rate	1114:1133	the degradation rate of the composite membrane	1114:1159	Furthermore, the degradation rate of the composite membrane was 60 % after 4 cycles.					
32829853	0	3	from	synthesis	7:15	arg1	degradation					90:100	the degradation	86:100	the degradation of tetrabromobisphenol A	86:125	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	1	4	theme	sulfate	145:151	arg1	process					180:186	a sulfate radical-advanced oxidation process	143:186	a sulfate radical-advanced oxidation process (SR-AOP)	143:195	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	4	theme	sulfate	145:151	arg1	SR-AOP					189:194	SR-AOP	189:194	SR-AOP	189:194	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	5	theme	metal-organic	242:254	arg1	Co/Fe					236:240	a bimetallic Co/Fe	223:240	a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF)	223:314	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	5	theme	metal-organic	242:254	arg1	catalyst					321:328	a catalyst	319:328	a catalyst for TBBPA degradation	319:350	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	6	6	theme	degradation	897:907	arg1	intermediates					909:921	the TBBPA degradation intermediates	887:921	the TBBPA degradation intermediates	887:921	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	6	theme	degradation	897:907	arg1	pathway					961:967	TBBPA degradation pathway	943:967	TBBPA degradation pathway	943:967	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	6	theme	degradation	897:907	arg1	recyclability					924:936	recyclability	924:936	recyclability	924:936	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	7	theme	factors	807:813	arg1	properties					756:765	The properties	752:765	The properties of the composite materials and the main factors that influenced TBBPA degradation	752:847	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	1	8	theme	frameworks/cellulose	256:275	arg1	membrane					287:294	frameworks/cellulose nanofiber membrane	256:294	frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF)	256:314	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	8	theme	frameworks/cellulose	256:275	arg1	bi-MOFs/CNF					303:313	Co/Fe bi-MOFs/CNF	297:313	Co/Fe bi-MOFs/CNF	297:313	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	6	9	theme	TBBPA	891:895	arg1	intermediates					909:921	the TBBPA degradation intermediates	887:921	the TBBPA degradation intermediates	887:921	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	9	theme	TBBPA	891:895	arg1	pathway					961:967	TBBPA degradation pathway	943:967	TBBPA degradation pathway	943:967	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	9	theme	TBBPA	891:895	arg1	recyclability					924:936	recyclability	924:936	recyclability	924:936	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	10	theme	main	802:805	arg1	factors					807:813	the main factors	798:813	the main factors	798:813	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	3	11	theme	facile	528:533	arg1	method					548:553	a facile solvothermal method	526:553	a facile solvothermal method	526:553	Co/Fe bi-MOFs/CNF was prepared by a facile solvothermal method and vacuum filtration.					
32829853	1	12	theme	nanofiber	277:285	arg1	membrane					287:294	frameworks/cellulose nanofiber membrane	256:294	frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF)	256:314	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	12	theme	nanofiber	277:285	arg1	bi-MOFs/CNF					303:313	Co/Fe bi-MOFs/CNF	297:313	Co/Fe bi-MOFs/CNF	297:313	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	0	13	theme	tetrabromobisphenol	105:123	arg1	A					125:125	tetrabromobisphenol A	105:125	tetrabromobisphenol A	105:125	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	8	14	theme	membrane	1152:1159	arg1	%					1168:1168	60 %	1165:1168	60 %	1165:1168	Furthermore, the degradation rate of the composite membrane was 60 % after 4 cycles.					
32829853	8	14	theme	membrane	1152:1159	arg1	rate					1130:1133	the degradation rate	1114:1133	the degradation rate of the composite membrane	1114:1159	Furthermore, the degradation rate of the composite membrane was 60 % after 4 cycles.					
32829853	1	15	theme	radical-advanced	153:168	arg1	process					180:186	a sulfate radical-advanced oxidation process	143:186	a sulfate radical-advanced oxidation process (SR-AOP)	143:195	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	15	theme	radical-advanced	153:168	arg1	SR-AOP					189:194	SR-AOP	189:194	SR-AOP	189:194	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	8	16	theme	composite	1142:1150	arg1	membrane					1152:1159	the composite membrane	1138:1159	the composite membrane	1138:1159	Furthermore, the degradation rate of the composite membrane was 60 % after 4 cycles.					
32829853	2	17	theme	hydroxyl	381:388	arg1	OH·					400:402	OH·	400:402	OH·	400:402	Sulfate radicals (SO4-) and hydroxyl radicals (OH·) were generated through the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF.					
32829853	2	17	theme	hydroxyl	381:388	arg1	radicals					390:397	hydroxyl radicals	381:397	hydroxyl radicals (OH·)	381:403	Sulfate radicals (SO4-) and hydroxyl radicals (OH·) were generated through the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF.					
32829853	6	18	theme	materials	784:792	arg1	properties					756:765	The properties	752:765	The properties of the composite materials and the main factors that influenced TBBPA degradation	752:847	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	4	19	theme	MOFs	660:663	arg1	agglomeration					636:648	the agglomeration	632:648	the agglomeration of loaded MOFs	632:663	CNF acted as a natural substrates material to relieve the agglomeration of loaded MOFs.					
32829853	0	20	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite	0:61	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	6	21	theme	composite	774:782	arg1	materials					784:792	the composite materials	770:792	the composite materials	770:792	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	3	22	theme	vacuum	559:564	arg1	filtration					566:575	vacuum filtration	559:575	vacuum filtration	559:575	Co/Fe bi-MOFs/CNF was prepared by a facile solvothermal method and vacuum filtration.					
32829853	4	23	theme	loaded	653:658	arg1	MOFs					660:663	loaded MOFs	653:663	loaded MOFs	653:663	CNF acted as a natural substrates material to relieve the agglomeration of loaded MOFs.					
32829853	7	24	theme	rate	1053:1056	arg1	min-1					1094:1098	0.764 min-1	1088:1098	0.764 min-1	1088:1098	Almost 100 % of TBBPA was degraded within 30 min under optimal conditions, and the rate constant was determined to be 0.764 min-1.					
32829853	7	24	theme	rate	1053:1056	arg1	constant					1058:1065	the rate constant	1049:1065	the rate constant	1049:1065	Almost 100 % of TBBPA was degraded within 30 min under optimal conditions, and the rate constant was determined to be 0.764 min-1.					
32829853	3	25	theme	solvothermal	535:546	arg1	method					548:553	a facile solvothermal method	526:553	a facile solvothermal method	526:553	Co/Fe bi-MOFs/CNF was prepared by a facile solvothermal method and vacuum filtration.					
32829853	1	26	theme	Co/Fe	297:301	arg1	membrane					287:294	frameworks/cellulose nanofiber membrane	256:294	frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF)	256:314	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	26	theme	Co/Fe	297:301	arg1	bi-MOFs/CNF					303:313	Co/Fe bi-MOFs/CNF	297:313	Co/Fe bi-MOFs/CNF	297:313	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	0	27	theme	A	125:125	arg1	degradation					90:100	the degradation	86:100	the degradation of tetrabromobisphenol A	86:125	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	2	28	theme	Sulfate	353:359	arg1	radicals					361:368	Sulfate radicals	353:368	Sulfate radicals (SO4-)	353:375	Sulfate radicals (SO4-) and hydroxyl radicals (OH·) were generated through the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF.					
32829853	2	28	theme	Sulfate	353:359	arg1	SO4-					371:374	SO4-	371:374	SO4-	371:374	Sulfate radicals (SO4-) and hydroxyl radicals (OH·) were generated through the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF.					
32829853	2	29	theme	peroxymonosulfate	446:462	arg1	activation					432:441	the activation	428:441	the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF	428:489	Sulfate radicals (SO4-) and hydroxyl radicals (OH·) were generated through the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF.					
32829853	4	30	theme	material	612:619	arg1	CNF					578:580	CNF	578:580	CNF	578:580	CNF acted as a natural substrates material to relieve the agglomeration of loaded MOFs.					
32829853	4	30	theme	material	612:619	arg1	substrates					601:610	a natural substrates	591:610	a natural substrates material to relieve the agglomeration of loaded MOFs	591:663	CNF acted as a natural substrates material to relieve the agglomeration of loaded MOFs.					
32829853	0	31	theme	bi-MOFs/CNF	28:38	arg1	nanocomposite					49:61	a Co/Fe bi-MOFs/CNF membrane nanocomposite	20:61	a Co/Fe bi-MOFs/CNF membrane nanocomposite	20:61	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	0	32	from	application	71:81	arg1	degradation					90:100	the degradation	86:100	the degradation of tetrabromobisphenol A	86:125	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	0	33	theme	Co/Fe	22:26	arg1	nanocomposite					49:61	a Co/Fe bi-MOFs/CNF membrane nanocomposite	20:61	a Co/Fe bi-MOFs/CNF membrane nanocomposite	20:61	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	6	34	theme	degradation	949:959	arg1	intermediates					909:921	the TBBPA degradation intermediates	887:921	the TBBPA degradation intermediates	887:921	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	34	theme	degradation	949:959	arg1	pathway					961:967	TBBPA degradation pathway	943:967	TBBPA degradation pathway	943:967	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	0	35	theme	nanocomposite	49:61	arg1	application					71:81	its application	67:81	its application in the degradation of tetrabromobisphenol A	67:125	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	0	35	theme	nanocomposite	49:61	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite	0:61	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	6	36	theme	TBBPA	943:947	arg1	intermediates					909:921	the TBBPA degradation intermediates	887:921	the TBBPA degradation intermediates	887:921	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	6	36	theme	TBBPA	943:947	arg1	pathway					961:967	TBBPA degradation pathway	943:967	TBBPA degradation pathway	943:967	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	0	37	theme	membrane	40:47	arg1	nanocomposite					49:61	a Co/Fe bi-MOFs/CNF membrane nanocomposite	20:61	a Co/Fe bi-MOFs/CNF membrane nanocomposite	20:61	Facile synthesis of a Co/Fe bi-MOFs/CNF membrane nanocomposite and its application in the degradation of tetrabromobisphenol A.					
32829853	3	38	theme	Co/Fe	492:496	arg1	bi-MOFs/CNF					498:508	Co/Fe bi-MOFs/CNF	492:508	Co/Fe bi-MOFs/CNF	492:508	Co/Fe bi-MOFs/CNF was prepared by a facile solvothermal method and vacuum filtration.					
32829853	5	39	theme	reaction	733:740	arg1	solution					742:749	the reaction solution	729:749	the reaction solution	729:749	Additionally the composite membranes was easily separated from the reaction solution.					
32829853	5	40	theme	composite	683:691	arg1	membranes					693:701	the composite membranes	679:701	the composite membranes	679:701	Additionally the composite membranes was easily separated from the reaction solution.					
32829853	6	41	theme	TBBPA	831:835	arg1	degradation					837:847	TBBPA degradation	831:847	TBBPA degradation	831:847	The properties of the composite materials and the main factors that influenced TBBPA degradation were elucidated in detail, along with the TBBPA degradation intermediates, recyclability, and TBBPA degradation pathway.					
32829853	7	42	theme	TBBPA	986:990	arg1	TBBPA					986:990	TBBPA	986:990	TBBPA	986:990	Almost 100 % of TBBPA was degraded within 30 min under optimal conditions, and the rate constant was determined to be 0.764 min-1.					
32829853	7	42	theme	TBBPA	986:990	arg1	%					981:981	Almost 100 %	970:981	Almost 100 % of TBBPA	970:990	Almost 100 % of TBBPA was degraded within 30 min under optimal conditions, and the rate constant was determined to be 0.764 min-1.					
32829853	1	43	dep	metal-organic	242:254	arg1	membrane					287:294	frameworks/cellulose nanofiber membrane	256:294	frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF)	256:314	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	43	dep	metal-organic	242:254	arg1	bi-MOFs/CNF					303:313	Co/Fe bi-MOFs/CNF	297:313	Co/Fe bi-MOFs/CNF	297:313	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	44	theme	oxidation	170:178	arg1	process					180:186	a sulfate radical-advanced oxidation process	143:186	a sulfate radical-advanced oxidation process (SR-AOP)	143:195	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	44	theme	oxidation	170:178	arg1	SR-AOP					189:194	SR-AOP	189:194	SR-AOP	189:194	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	2	45	theme	Co/Fe	473:477	arg1	bi-MOFs/CNF					479:489	Co/Fe bi-MOFs/CNF	473:489	Co/Fe bi-MOFs/CNF	473:489	Sulfate radicals (SO4-) and hydroxyl radicals (OH·) were generated through the activation of peroxymonosulfate (PMS) by Co/Fe bi-MOFs/CNF.					
32829853	4	46	theme	natural	593:599	arg1	CNF					578:580	CNF	578:580	CNF	578:580	CNF acted as a natural substrates material to relieve the agglomeration of loaded MOFs.					
32829853	4	46	theme	natural	593:599	arg1	substrates					601:610	a natural substrates	591:610	a natural substrates material to relieve the agglomeration of loaded MOFs	591:663	CNF acted as a natural substrates material to relieve the agglomeration of loaded MOFs.					
32829853	1	47	theme	bimetallic	225:234	arg1	Co/Fe					236:240	a bimetallic Co/Fe	223:240	a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF)	223:314	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32829853	1	47	theme	bimetallic	225:234	arg1	catalyst					321:328	a catalyst	319:328	a catalyst for TBBPA degradation	319:350	In this study, a sulfate radical-advanced oxidation process (SR-AOP) was proposed by utilizing a bimetallic Co/Fe metal-organic frameworks/cellulose nanofiber membrane (Co/Fe bi-MOFs/CNF) as a catalyst for TBBPA degradation.					
32567068	2	0	theme	dispersive	587:596	arg1	spectroscopy					604:615	energy dispersive X-ray spectroscopy	580:615	energy dispersive X-ray spectroscopy	580:615	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	6	1	theme	bacterial	1281:1289	arg1	surface					1291:1297	bacterial surface	1281:1297	bacterial surface	1281:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	8	2	theme	high	1709:1712	arg1	weight					1724:1729	high molecular weight	1709:1729	high molecular weight total protein	1709:1743	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	1	3	theme	casting	259:265	arg1	method					267:272	a casting method	257:272	a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers	257:340	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	7	4	theme	weight	1489:1494	arg1	synthesis					1461:1469	the synthesis	1457:1469	the synthesis of high molecular weight total protein	1457:1508	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	6	5	theme	charge	1271:1276	arg1	NH3+					1243:1246	NH3+	1243:1246	NH3+ of CS and the negative charge on bacterial surface	1243:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	8	6	theme	weight	1724:1729	arg1	protein					1737:1743	high molecular weight total protein	1709:1743	high molecular weight total protein	1709:1743	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	7	7	theme	high	1474:1477	arg1	weight					1489:1494	high molecular weight	1474:1494	high molecular weight total protein	1474:1508	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	7	8	theme	ion	1426:1428	arg1	interaction					1430:1440	the earlier-mentioned ion interaction	1404:1440	the earlier-mentioned ion interaction	1404:1440	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	9	9	theme	antimicrobial	1828:1840	arg1	materials					1842:1850	antimicrobial materials	1828:1850	antimicrobial materials	1828:1850	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	9	9	theme	antimicrobial	1828:1840	arg1	coatings					1815:1822	Ag/ZnO-CS composite coatings	1795:1822	Ag/ZnO-CS composite coatings	1795:1822	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	8	10	theme	protein	1737:1743	arg1	synthesis					1696:1704	the synthesis	1692:1704	the synthesis of high molecular weight total protein and low molecular weight membrane protein	1692:1785	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	6	11	theme	cell	1160:1163	arg1	membrane					1165:1172	the bacterial cell membrane	1146:1172	the bacterial cell membrane	1146:1172	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	6	12	theme	CS	1251:1252	arg1	NH3+					1243:1246	NH3+	1243:1246	NH3+ of CS and the negative charge on bacterial surface	1243:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	8	13	theme	low	1749:1751	arg1	weight					1763:1768	low molecular weight	1749:1768	low molecular weight	1749:1768	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	1	14	theme	chitosan	204:211	arg1	coatings					218:225	the chitosan (CS) coatings	200:225	the chitosan (CS) coatings	200:225	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	2	15	theme	electron	556:563	arg1	microscopy					565:574	transmission electron microscopy	543:574	transmission electron microscopy	543:574	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	3	16	theme	antibacterial	757:769	arg1	activities					771:780	the antibacterial activities	753:780	the antibacterial activities	753:780	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	5	17	theme	0.5	1086:1088	arg1	%					1089:1089	%	1089:1089	%	1089:1089	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	8	18	theme	weight	1763:1768	arg1	synthesis					1696:1704	the synthesis	1692:1704	the synthesis of high molecular weight total protein and low molecular weight membrane protein	1692:1785	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	3	19	theme	CS	803:804	arg1	coatings					806:813	the CS coatings	799:813	the CS coatings	799:813	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	6	20	from	CS	1251:1252	arg1	surface					1291:1297	bacterial surface	1281:1297	bacterial surface	1281:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	5	21	theme	Ag/ZnO-CS	1091:1099	arg1	coating					1101:1107	0.5% Ag/ZnO-CS coating	1086:1107	0.5% Ag/ZnO-CS coating	1086:1107	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	4	22	theme	CS	927:928	arg1	coatings					930:937	the CS coatings	923:937	the CS coatings	923:937	RESULTS The results show that ZnO and Ag/ZnO microspheres are dispersed homogeneously in the CS coatings.					
32567068	1	23	theme	oxide	284:288	arg1	microspheres					316:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres	279:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers	279:340	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	5	24	dep	properties	1002:1011	arg1	the					987:989	the	987:989	the	987:989	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	7	25	theme	cell	1357:1360	arg1	membrane					1362:1369	the bacterial cell membrane	1343:1369	the bacterial cell membrane	1343:1369	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	6	26	theme	electrostatic	1209:1221	arg1	interaction					1223:1233	the electrostatic interaction	1205:1233	the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface	1205:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	3	27	theme	dominant	641:648	arg1	aeruginosa					729:738	Pseudomonas aeruginosa	717:738	Pseudomonas aeruginosa	717:738	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	27	theme	dominant	641:648	arg1	objects					744:750	objects	744:750	objects	744:750	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	27	theme	dominant	641:648	arg1	bacteria					659:666	the dominant spoilage bacteria	637:666	the dominant spoilage bacteria of aquatic products	637:686	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	27	theme	dominant	641:648	arg1	putrefaciens					700:711	putrefaciens	700:711	putrefaciens	700:711	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	1	28	theme	physicochemical	142:156	arg1	properties					176:185	the physicochemical and antibacterial properties	138:185	the physicochemical and antibacterial properties of coatings	138:197	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	2	29	theme	infrared	490:497	arg1	spectroscopy					499:510	Fourier-transform infrared spectroscopy	472:510	Fourier-transform infrared spectroscopy	472:510	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	6	30	from	NH3+	1243:1246	arg1	surface					1291:1297	bacterial surface	1281:1297	bacterial surface	1281:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	0	31	theme	chitosan	65:72	arg1	coatings					74:81	the chitosan coatings	61:81	the chitosan coatings	61:81	Preparation, characterization and antibacterial mechanism of the chitosan coatings modified by Ag/ZnO microspheres.					
32567068	1	32	theme	antibacterial	162:174	arg1	properties					176:185	the physicochemical and antibacterial properties	138:185	the physicochemical and antibacterial properties of coatings	138:197	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	1	33	theme	/ZnO	311:314	arg1	microspheres					316:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres	279:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers	279:340	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	2	34	theme	electron	522:529	arg1	microscopy					531:540	scanning electron microscopy	513:540	scanning electron microscopy	513:540	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	0	35	theme	Ag/ZnO	95:100	arg1	microspheres					102:113	Ag/ZnO microspheres	95:113	Ag/ZnO microspheres	95:113	Preparation, characterization and antibacterial mechanism of the chitosan coatings modified by Ag/ZnO microspheres.					
32567068	5	36	theme	mechanical	991:1000	arg1	properties					1002:1011	mechanical properties	991:1011	mechanical properties	991:1011	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	9	37	theme	composite	1805:1813	arg1	materials					1842:1850	antimicrobial materials	1828:1850	antimicrobial materials	1828:1850	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	9	37	theme	composite	1805:1813	arg1	coatings					1815:1822	Ag/ZnO-CS composite coatings	1795:1822	Ag/ZnO-CS composite coatings	1795:1822	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	9	38	theme	food	1856:1859	arg1	materials					1874:1882	food preservative materials	1856:1882	food preservative materials	1856:1882	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	2	39	theme	coatings	422:429	arg1	micromorphology					371:385	micromorphology	371:385	micromorphology of ZnO, Ag/ZnO microspheres and CS coatings	371:429	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	2	39	theme	coatings	422:429	arg1	structures					356:365	The chemical structures	343:365	The chemical structures	343:365	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	8	40	theme	composite	1547:1555	arg1	membrane					1585:1592	the bacterial cell membrane	1566:1592	the bacterial cell membrane	1566:1592	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	8	40	theme	composite	1547:1555	arg1	coating					1557:1563	Ag/ZnO-CS composite coating	1537:1563	Ag/ZnO-CS composite coating	1537:1563	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	1	41	theme	zinc	279:282	arg1	Ag					308:309	Ag	308:309	Ag	308:309	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	1	41	theme	zinc	279:282	arg1	ZnO					291:293	ZnO	291:293	ZnO	291:293	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	1	41	theme	zinc	279:282	arg1	oxide					284:288	zinc oxide	279:288	zinc oxide (ZnO)	279:294	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	7	42	theme	composite	1324:1332	arg1	coating					1334:1340	ZnO-CS composite coating	1317:1340	ZnO-CS composite coating	1317:1340	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	2	43	theme	Ag/ZnO	395:400	arg1	microspheres					402:413	ZnO, Ag/ZnO microspheres	390:413	ZnO, Ag/ZnO microspheres	390:413	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	4	44	dep	ZnO	864:866	arg1	microspheres					879:890	microspheres	879:890	microspheres	879:890	RESULTS The results show that ZnO and Ag/ZnO microspheres are dispersed homogeneously in the CS coatings.					
32567068	2	45	theme	X-ray	453:457	arg1	diffraction					459:469	X-ray diffraction	453:469	X-ray diffraction	453:469	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	8	46	theme	bacterial	1570:1578	arg1	membrane					1585:1592	the bacterial cell membrane	1566:1592	the bacterial cell membrane	1566:1592	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	8	46	theme	bacterial	1570:1578	arg1	coating					1557:1563	Ag/ZnO-CS composite coating	1537:1563	Ag/ZnO-CS composite coating	1537:1563	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	3	47	theme	aquatic	671:677	arg1	products					679:686	aquatic products	671:686	aquatic products	671:686	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	0	48	theme	antibacterial	34:46	arg1	mechanism					48:56	antibacterial mechanism	34:56	antibacterial mechanism	34:56	Preparation, characterization and antibacterial mechanism of the chitosan coatings modified by Ag/ZnO microspheres.					
32567068	5	49	theme	coatings	1051:1058	arg1	properties					1002:1011	mechanical properties	991:1011	mechanical properties	991:1011	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	5	49	theme	coatings	1051:1058	arg1	abilities					1031:1039	antibacterial abilities	1017:1039	antibacterial abilities	1017:1039	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	1	50	with	method	267:272	arg1	microspheres					316:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres	279:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers	279:340	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	1	51	theme	silver	300:305	arg1	microspheres					316:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres	279:327	zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers	279:340	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	6	52	theme	CS	1134:1135	arg1	coating					1137:1143	pure CS coating	1129:1143	pure CS coating	1129:1143	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	9	53	theme	great	1889:1893	arg1	application					1905:1915	great potential application	1889:1915	great potential application	1889:1915	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	8	54	theme	ion	1664:1666	arg1	interaction					1668:1678	ion interaction	1664:1678	ion interaction	1664:1678	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	6	55	from	surface	1291:1297	arg1	NH3+					1243:1246	NH3+	1243:1246	NH3+ of CS and the negative charge on bacterial surface	1243:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	7	56	theme	total	1496:1500	arg1	protein					1502:1508	total protein	1496:1508	high molecular weight total protein	1474:1508	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	7	57	theme	molecular	1479:1487	arg1	weight					1489:1494	high molecular weight	1474:1494	high molecular weight total protein	1474:1508	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	10	58	theme	Chemical	1936:1943	arg1	Industry					1945:1952	Chemical Industry	1936:1952	Chemical Industry	1936:1952	© 2020 Society of Chemical Industry.					
32567068	9	59	with	materials	1874:1882	arg1	application					1905:1915	great potential application	1889:1915	great potential application	1889:1915	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	2	60	theme	X-ray	598:602	arg1	spectroscopy					604:615	energy dispersive X-ray spectroscopy	580:615	energy dispersive X-ray spectroscopy	580:615	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	6	61	theme	negative	1262:1269	arg1	charge					1271:1276	the negative charge	1258:1276	the negative charge on bacterial surface	1258:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	8	62	theme	molecular	1714:1722	arg1	weight					1724:1729	high molecular weight	1709:1729	high molecular weight total protein	1709:1743	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	1	63	theme	coatings	190:197	arg1	properties					176:185	the physicochemical and antibacterial properties	138:185	the physicochemical and antibacterial properties of coatings	138:197	BACKGROUND To improve the physicochemical and antibacterial properties of coatings, the chitosan (CS) coatings were respectively prepared by a casting method with zinc oxide (ZnO) and silver (Ag)/ZnO microspheres as modifiers.					
32567068	9	64	with	materials	1842:1850	arg1	application					1905:1915	great potential application	1889:1915	great potential application	1889:1915	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	8	65	theme	total	1731:1735	arg1	protein					1737:1743	high molecular weight total protein	1709:1743	high molecular weight total protein	1709:1743	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	7	66	theme	earlier-mentioned	1408:1424	arg1	interaction					1430:1440	the earlier-mentioned ion interaction	1404:1440	the earlier-mentioned ion interaction	1404:1440	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	6	67	theme	bacterial	1150:1158	arg1	membrane					1165:1172	the bacterial cell membrane	1146:1172	the bacterial cell membrane	1146:1172	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	2	68	theme	transmission	543:554	arg1	microscopy					565:574	transmission electron microscopy	543:574	transmission electron microscopy	543:574	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	5	69	theme	%	1089:1089	arg1	coating					1101:1107	0.5% Ag/ZnO-CS coating	1086:1107	0.5% Ag/ZnO-CS coating	1086:1107	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	8	70	theme	molecular	1753:1761	arg1	weight					1763:1768	low molecular weight	1749:1768	low molecular weight	1749:1768	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	3	71	theme	coatings	806:813	arg1	mechanism					786:794	mechanism	786:794	mechanism	786:794	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	71	theme	coatings	806:813	arg1	activities					771:780	the antibacterial activities	753:780	the antibacterial activities	753:780	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	8	72	theme	membrane	1770:1777	arg1	protein					1779:1785	membrane protein	1770:1785	membrane protein	1770:1785	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	2	73	theme	energy	580:585	arg1	spectroscopy					604:615	energy dispersive X-ray spectroscopy	580:615	energy dispersive X-ray spectroscopy	580:615	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	0	74	theme	coatings	74:81	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and antibacterial mechanism of the chitosan coatings modified by Ag/ZnO microspheres.					
32567068	0	74	theme	coatings	74:81	arg1	mechanism					48:56	antibacterial mechanism	34:56	antibacterial mechanism	34:56	Preparation, characterization and antibacterial mechanism of the chitosan coatings modified by Ag/ZnO microspheres.					
32567068	0	74	theme	coatings	74:81	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and antibacterial mechanism of the chitosan coatings modified by Ag/ZnO microspheres.					
32567068	2	75	theme	Fourier-transform	472:488	arg1	spectroscopy					499:510	Fourier-transform infrared spectroscopy	472:510	Fourier-transform infrared spectroscopy	472:510	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	7	76	theme	bacterial	1347:1355	arg1	membrane					1362:1369	the bacterial cell membrane	1343:1369	the bacterial cell membrane	1343:1369	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	9	77	theme	Ag/ZnO-CS	1795:1803	arg1	materials					1842:1850	antimicrobial materials	1828:1850	antimicrobial materials	1828:1850	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	9	77	theme	Ag/ZnO-CS	1795:1803	arg1	coatings					1815:1822	Ag/ZnO-CS composite coatings	1795:1822	Ag/ZnO-CS composite coatings	1795:1822	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	2	78	theme	scanning	513:520	arg1	microscopy					531:540	scanning electron microscopy	513:540	scanning electron microscopy	513:540	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	8	79	theme	Ag/ZnO-CS	1537:1545	arg1	membrane					1585:1592	the bacterial cell membrane	1566:1592	the bacterial cell membrane	1566:1592	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	8	79	theme	Ag/ZnO-CS	1537:1545	arg1	coating					1557:1563	Ag/ZnO-CS composite coating	1537:1563	Ag/ZnO-CS composite coating	1537:1563	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	5	80	dep	ZnO	958:960	arg1	microspheres					973:984	microspheres	973:984	microspheres	973:984	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	8	81	dep	protein	1737:1743	arg1	protein					1779:1785	membrane protein	1770:1785	membrane protein	1770:1785	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	7	82	theme	ZnO-CS	1317:1322	arg1	coating					1334:1340	ZnO-CS composite coating	1317:1340	ZnO-CS composite coating	1317:1340	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	2	83	theme	CS	419:420	arg1	coatings					422:429	CS coatings	419:429	CS coatings	419:429	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	2	84	theme	chemical	347:354	arg1	structures					356:365	The chemical structures	343:365	The chemical structures	343:365	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	7	85	dep	weight	1489:1494	arg1	protein					1502:1508	total protein	1496:1508	high molecular weight total protein	1474:1508	After treated by ZnO-CS composite coating, the bacterial cell membrane is destroyed badly on account of the earlier-mentioned ion interaction and disturbing the synthesis of high molecular weight total protein.					
32567068	2	86	theme	microspheres	402:413	arg1	micromorphology					371:385	micromorphology	371:385	micromorphology of ZnO, Ag/ZnO microspheres and CS coatings	371:429	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	2	86	theme	microspheres	402:413	arg1	structures					356:365	The chemical structures	343:365	The chemical structures	343:365	The chemical structures and micromorphology of ZnO, Ag/ZnO microspheres and CS coatings were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy.					
32567068	8	87	theme	cell	1580:1583	arg1	membrane					1585:1592	the bacterial cell membrane	1566:1592	the bacterial cell membrane	1566:1592	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	8	87	theme	cell	1580:1583	arg1	coating					1557:1563	Ag/ZnO-CS composite coating	1537:1563	Ag/ZnO-CS composite coating	1537:1563	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	5	88	theme	CS	1048:1049	arg1	coatings					1051:1058	the CS coatings	1044:1058	the CS coatings	1044:1058	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	3	89	theme	spoilage	650:657	arg1	aeruginosa					729:738	Pseudomonas aeruginosa	717:738	Pseudomonas aeruginosa	717:738	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	89	theme	spoilage	650:657	arg1	objects					744:750	objects	744:750	objects	744:750	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	89	theme	spoilage	650:657	arg1	bacteria					659:666	the dominant spoilage bacteria	637:666	the dominant spoilage bacteria of aquatic products	637:686	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	89	theme	spoilage	650:657	arg1	putrefaciens					700:711	putrefaciens	700:711	putrefaciens	700:711	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	4	90	dep	RESULTS	834:840	arg1	show					854:857	show	854:857	show that ZnO and Ag/ZnO microspheres are dispersed homogeneously in the CS coatings	854:937	RESULTS The results show that ZnO and Ag/ZnO microspheres are dispersed homogeneously in the CS coatings.					
32567068	5	91	theme	antibacterial	1017:1029	arg1	abilities					1031:1039	antibacterial abilities	1017:1039	antibacterial abilities	1017:1039	After modified by ZnO and Ag/ZnO microspheres, the mechanical properties and antibacterial abilities of the CS coatings are improved, and that of 0.5% Ag/ZnO-CS coating is the optimal.					
32567068	6	92	theme	pure	1129:1132	arg1	coating					1137:1143	pure CS coating	1129:1143	pure CS coating	1129:1143	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32567068	9	93	theme	potential	1895:1903	arg1	application					1905:1915	great potential application	1889:1915	great potential application	1889:1915	Hence, Ag/ZnO-CS composite coatings are antimicrobial materials and food preservative materials with great potential application.					
32567068	8	94	theme	cell	1619:1622	arg1	contents					1624:1631	cell contents	1619:1631	cell contents	1619:1631	CONCLUSION With regard to Ag/ZnO-CS composite coating, the bacterial cell membrane is damaged seriously and cell contents are completely released due to ion interaction, disturbing the synthesis of high molecular weight total protein and low molecular weight membrane protein.					
32567068	3	95	theme	products	679:686	arg1	aeruginosa					729:738	Pseudomonas aeruginosa	717:738	Pseudomonas aeruginosa	717:738	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	95	theme	products	679:686	arg1	objects					744:750	objects	744:750	objects	744:750	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	95	theme	products	679:686	arg1	bacteria					659:666	the dominant spoilage bacteria	637:666	the dominant spoilage bacteria of aquatic products	637:686	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	3	95	theme	products	679:686	arg1	putrefaciens					700:711	putrefaciens	700:711	putrefaciens	700:711	Furthermore, using the dominant spoilage bacteria of aquatic products, Shewanella putrefaciens and Pseudomonas aeruginosa, as objects, the antibacterial activities and mechanism of the CS coatings were investigated.					
32567068	6	96	from	charge	1271:1276	arg1	surface					1291:1297	bacterial surface	1281:1297	bacterial surface	1281:1297	For pure CS coating, the bacterial cell membrane is damaged slightly because of the electrostatic interaction between NH3+ of CS and the negative charge on bacterial surface.					
32844867	3	0	theme	transmission	704:715	arg1	microscopy					726:735	field emission transmission electron microscopy	689:735	field emission transmission electron microscopy (FEG-TEM)	689:745	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	0	theme	transmission	704:715	arg1	FEG-TEM					738:744	FEG-TEM	738:744	FEG-TEM	738:744	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	1	1	theme	electrochemical	171:185	arg1	sensor					187:192	an electrochemical sensor	168:192	an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin	168:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	0	2	theme	efficient	96:104	arg1	platform					106:113	an efficient platform	93:113	an efficient platform for electrochemical sensing	93:141	Tin disulfide-graphene oxide-β-cyclodextrin mediated electro-oxidation of melatonin hormone: an efficient platform for electrochemical sensing.					
32844867	1	3	theme	ternary	327:333	arg1	nanocomposite					335:347	a ternary nanocomposite	325:347	a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin	325:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	4	4	theme	electrode	796:804	arg1	performance					768:778	The electrochemical performance	748:778	The electrochemical performance of the modified electrode	748:804	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	1	5	theme	graphene	270:277	arg1	oxide					279:283	graphene oxide	270:283	graphene oxide (GO)	270:288	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	0	6	theme	electrochemical	119:133	arg1	sensing					135:141	electrochemical sensing	119:141	electrochemical sensing	119:141	Tin disulfide-graphene oxide-β-cyclodextrin mediated electro-oxidation of melatonin hormone: an efficient platform for electrochemical sensing.					
32844867	3	7	theme	field	689:693	arg1	microscopy					726:735	field emission transmission electron microscopy	689:735	field emission transmission electron microscopy (FEG-TEM)	689:745	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	7	theme	field	689:693	arg1	FEG-TEM					738:744	FEG-TEM	738:744	FEG-TEM	738:744	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	1	8	theme	melatonin	432:440	arg1	detection					419:427	the electrochemical detection	399:427	the electrochemical detection of melatonin	399:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	5	9	theme	1	1002:1002	arg1	nM					1004:1005	nM	1004:1005	nM	1004:1005	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	2	10	theme	ternary	513:519	arg1	composite					521:529	the ternary composite	509:529	the ternary composite	509:529	Hydrothermally synthesized SnS2 was mixed with GO/β-CD to prepare the ternary composite via an ultra-sonication process.					
32844867	4	11	theme	modified	787:794	arg1	electrode					796:804	the modified electrode	783:804	the modified electrode	783:804	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	6	12	theme	Immuno-Sorbent	1222:1235	arg1	ELISA					1244:1248	ELISA	1244:1248	ELISA	1244:1248	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	6	12	theme	Immuno-Sorbent	1222:1235	arg1	Assay					1237:1241	the Enzyme-Linked Immuno-Sorbent Assay	1204:1241	the Enzyme-Linked Immuno-Sorbent Assay (ELISA)	1204:1249	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	0	13	theme	melatonin	74:82	arg1	hormone					84:90	melatonin hormone	74:90	melatonin hormone	74:90	Tin disulfide-graphene oxide-β-cyclodextrin mediated electro-oxidation of melatonin hormone: an efficient platform for electrochemical sensing.					
32844867	3	14	theme	electron	717:724	arg1	microscopy					726:735	field emission transmission electron microscopy	689:735	field emission transmission electron microscopy (FEG-TEM)	689:745	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	14	theme	electron	717:724	arg1	FEG-TEM					738:744	FEG-TEM	738:744	FEG-TEM	738:744	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	0	15	theme	disulfide-graphene	4:21	arg1	oxide-β-cyclodextrin					23:42	Tin disulfide-graphene oxide-β-cyclodextrin	0:42	Tin disulfide-graphene oxide-β-cyclodextrin	0:42	Tin disulfide-graphene oxide-β-cyclodextrin mediated electro-oxidation of melatonin hormone: an efficient platform for electrochemical sensing.					
32844867	1	16	theme	screen-printed	364:377	arg1	SPE					390:392	SPE	390:392	SPE	390:392	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	16	theme	screen-printed	364:377	arg1	electrode					379:387	a screen-printed electrode	362:387	a screen-printed electrode (SPE) for the electrochemical detection of melatonin	362:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	4	17	theme	impedance	870:878	arg1	EIS					894:896	EIS	894:896	EIS	894:896	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	4	17	theme	impedance	870:878	arg1	spectroscopy					880:891	electrochemical impedance spectroscopy	854:891	electrochemical impedance spectroscopy (EIS)	854:897	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	1	18	theme	integrated	203:212	arg1	oxide					279:283	graphene oxide	270:283	graphene oxide (GO)	270:288	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	18	theme	integrated	203:212	arg1	nanoflakes					258:267	tin disulfide (SnS2) nanoflakes	237:267	tin disulfide (SnS2) nanoflakes	237:267	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	18	theme	integrated	203:212	arg1	β-CD					311:314	β-CD	311:314	β-CD	311:314	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	18	theme	integrated	203:212	arg1	β-cyclodextrin					295:308	β-cyclodextrin	295:308	β-cyclodextrin	295:308	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	18	theme	integrated	203:212	arg1	materials					217:225	integrated 2D materials	203:225	integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin	203:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	0	19	theme	Tin	0:2	arg1	oxide-β-cyclodextrin					23:42	Tin disulfide-graphene oxide-β-cyclodextrin	0:42	Tin disulfide-graphene oxide-β-cyclodextrin	0:42	Tin disulfide-graphene oxide-β-cyclodextrin mediated electro-oxidation of melatonin hormone: an efficient platform for electrochemical sensing.					
32844867	4	20	theme	electrochemical	854:868	arg1	EIS					894:896	EIS	894:896	EIS	894:896	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	4	20	theme	electrochemical	854:868	arg1	spectroscopy					880:891	electrochemical impedance spectroscopy	854:891	electrochemical impedance spectroscopy (EIS)	854:897	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	5	21	theme	lowest	1026:1031	arg1	limit					1043:1047	the lowest detection limit	1022:1047	the lowest detection limit of 0.17 nM	1022:1058	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	1	22	theme	2D	214:215	arg1	oxide					279:283	graphene oxide	270:283	graphene oxide (GO)	270:288	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	22	theme	2D	214:215	arg1	nanoflakes					258:267	tin disulfide (SnS2) nanoflakes	237:267	tin disulfide (SnS2) nanoflakes	237:267	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	22	theme	2D	214:215	arg1	β-CD					311:314	β-CD	311:314	β-CD	311:314	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	22	theme	2D	214:215	arg1	β-cyclodextrin					295:308	β-cyclodextrin	295:308	β-cyclodextrin	295:308	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	1	22	theme	2D	214:215	arg1	materials					217:225	integrated 2D materials	203:225	integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin	203:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	4	23	theme	electrochemical	752:766	arg1	performance					768:778	The electrochemical performance	748:778	The electrochemical performance of the modified electrode	748:804	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	6	24	theme	Enzyme-Linked	1208:1220	arg1	ELISA					1244:1248	ELISA	1244:1248	ELISA	1244:1248	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	6	24	theme	Enzyme-Linked	1208:1220	arg1	Assay					1237:1241	the Enzyme-Linked Immuno-Sorbent Assay	1204:1241	the Enzyme-Linked Immuno-Sorbent Assay (ELISA)	1204:1249	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	5	25	dep	nM	1004:1005	arg1	to					1007:1008	to	1007:1008	to	1007:1008	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	6	26	theme	commercial	1131:1140	arg1	drugs					1142:1146	commercial drugs	1131:1146	commercial drugs	1131:1146	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	5	27	theme	linearity	981:989	arg1	range					991:995	a linearity range	979:995	a linearity range from 1 nM to 100 μM	979:1015	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	3	28	theme	electron	655:662	arg1	FE-SEM					676:681	FE-SEM	676:681	FE-SEM	676:681	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	28	theme	electron	655:662	arg1	microscopy					664:673	field emission scanning electron microscopy	631:673	field emission scanning electron microscopy (FE-SEM)	631:682	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	6	29	theme	melatonin	1118:1126	arg1	detection					1105:1113	the detection	1101:1113	the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA)	1101:1249	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	2	30	theme	ultra-sonication	538:553	arg1	process					555:561	an ultra-sonication process	535:561	an ultra-sonication process	535:561	Hydrothermally synthesized SnS2 was mixed with GO/β-CD to prepare the ternary composite via an ultra-sonication process.					
32844867	5	31	theme	nM	1057:1058	arg1	limit					1043:1047	the lowest detection limit	1022:1047	the lowest detection limit of 0.17 nM	1022:1058	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	5	32	theme	100	1010:1012	arg1	nM					1004:1005	nM	1004:1005	nM	1004:1005	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	5	33	from	μM	1014:1015	arg1	range					991:995	a linearity range	979:995	a linearity range from 1 nM to 100 μM	979:1015	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	1	34	theme	tin	237:239	arg1	nanoflakes					258:267	tin disulfide (SnS2) nanoflakes	237:267	tin disulfide (SnS2) nanoflakes	237:267	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	3	35	theme	emission	637:644	arg1	FE-SEM					676:681	FE-SEM	676:681	FE-SEM	676:681	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	35	theme	emission	637:644	arg1	microscopy					664:673	field emission scanning electron microscopy	631:673	field emission scanning electron microscopy (FE-SEM)	631:682	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	4	36	theme	cyclic	829:834	arg1	voltammetry					836:846	cyclic voltammetry	829:846	cyclic voltammetry (CV)	829:851	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	4	36	theme	cyclic	829:834	arg1	CV					849:850	CV	849:850	CV	849:850	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	1	37	theme	disulfide	241:249	arg1	nanoflakes					258:267	tin disulfide (SnS2) nanoflakes	237:267	tin disulfide (SnS2) nanoflakes	237:267	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	0	38	theme	hormone	84:90	arg1	electro-oxidation					53:69	electro-oxidation	53:69	electro-oxidation of melatonin hormone	53:90	Tin disulfide-graphene oxide-β-cyclodextrin mediated electro-oxidation of melatonin hormone: an efficient platform for electrochemical sensing.					
32844867	3	39	theme	scanning	646:653	arg1	FE-SEM					676:681	FE-SEM	676:681	FE-SEM	676:681	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	39	theme	scanning	646:653	arg1	microscopy					664:673	field emission scanning electron microscopy	631:673	field emission scanning electron microscopy (FE-SEM)	631:682	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	2	40	theme	synthesized	458:468	arg1	SnS2					470:473	Hydrothermally synthesized SnS2	443:473	Hydrothermally synthesized SnS2	443:473	Hydrothermally synthesized SnS2 was mixed with GO/β-CD to prepare the ternary composite via an ultra-sonication process.					
32844867	1	41	dep	oxide	279:283	arg1	GO					286:287	GO	286:287	GO	286:287	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	3	42	theme	X-ray	606:610	arg1	diffraction					612:622	X-ray diffraction	606:622	X-ray diffraction (XRD)	606:628	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	42	theme	X-ray	606:610	arg1	XRD					625:627	XRD	625:627	XRD	625:627	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	4	43	theme	pulse	917:921	arg1	DPV					936:938	DPV	936:938	DPV	936:938	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	4	43	theme	pulse	917:921	arg1	voltammetry					923:933	differential pulse voltammetry	904:933	differential pulse voltammetry (DPV)	904:939	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	5	44	theme	detection	1033:1041	arg1	limit					1043:1047	the lowest detection limit	1022:1047	the lowest detection limit of 0.17 nM	1022:1058	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	6	45	theme	consistent	1181:1190	arg1	result					1192:1197	a consistent result	1179:1197	a consistent result	1179:1197	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	6	46	from	detection	1105:1113	arg1	drugs					1142:1146	commercial drugs	1131:1146	commercial drugs	1131:1146	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	6	46	from	detection	1105:1113	arg1	saliva					1158:1163	human saliva	1152:1163	human saliva	1152:1163	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	5	47	theme	electrochemical	946:960	arg1	sensor					962:967	The electrochemical sensor	942:967	The electrochemical sensor	942:967	The electrochemical sensor exhibited a linearity range from 1 nM to 100 μM with the lowest detection limit of 0.17 nM.					
32844867	4	48	theme	differential	904:915	arg1	DPV					936:938	DPV	936:938	DPV	936:938	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	4	48	theme	differential	904:915	arg1	voltammetry					923:933	differential pulse voltammetry	904:933	differential pulse voltammetry (DPV)	904:939	The electrochemical performance of the modified electrode was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).					
32844867	1	49	theme	electrochemical	403:417	arg1	detection					419:427	the electrochemical detection	399:427	the electrochemical detection of melatonin	399:440	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	3	50	theme	field	631:635	arg1	FE-SEM					676:681	FE-SEM	676:681	FE-SEM	676:681	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	50	theme	field	631:635	arg1	microscopy					664:673	field emission scanning electron microscopy	631:673	field emission scanning electron microscopy (FE-SEM)	631:682	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	1	51	theme	SnS2	252:255	arg1	nanoflakes					258:267	tin disulfide (SnS2) nanoflakes	237:267	tin disulfide (SnS2) nanoflakes	237:267	Here, we have developed an electrochemical sensor based on integrated 2D materials including tin disulfide (SnS2) nanoflakes, graphene oxide (GO), and β-cyclodextrin (β-CD) forming a ternary nanocomposite decorated on a screen-printed electrode (SPE) for the electrochemical detection of melatonin.					
32844867	6	52	theme	human	1152:1156	arg1	saliva					1158:1163	human saliva	1152:1163	human saliva	1152:1163	The sensor was successfully applied for the detection of melatonin in commercial drugs and human saliva, which showed a consistent result with the Enzyme-Linked Immuno-Sorbent Assay (ELISA).					
32844867	3	53	theme	emission	695:702	arg1	microscopy					726:735	field emission transmission electron microscopy	689:735	field emission transmission electron microscopy (FEG-TEM)	689:745	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32844867	3	53	theme	emission	695:702	arg1	FEG-TEM					738:744	FEG-TEM	738:744	FEG-TEM	738:744	The nanocomposite was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and field emission transmission electron microscopy (FEG-TEM).					
32217095	4	0	theme	product	912:918	arg1	properties					884:893	the spectroscopic properties	866:893	the spectroscopic properties of substrate and product	866:918	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	2	1	theme	proteins	382:389	arg1	presence					363:370	the presence	359:370	the presence of plasma proteins, amino acids, as well as other components of plasma	359:441	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	1	2	theme	blood	173:177	arg1	plasma					179:184	blood plasma	173:184	blood plasma	173:184	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	2	3	theme	plasma	375:380	arg1	proteins					382:389	plasma proteins	375:389	plasma proteins	375:389	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	3	4	theme	activity	534:541	arg1	measurement					509:519	continuous real-time measurement	488:519	continuous real-time measurement of catalytic activity of l-asparaginase	488:559	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	2	5	theme	Existing	315:322	arg1	methods					324:330	Existing methods	315:330	Existing methods	315:330	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	1	6	theme	enzymes	280:286	arg1	parameters					261:270	the catalytic parameters	247:270	the catalytic parameters	247:270	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	6	theme	enzymes	280:286	arg1	l-asparaginase					299:312	l-asparaginase	299:312	l-asparaginase	299:312	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	0	7	theme	blood	108:112	arg1	plasma					114:119	blood plasma	108:119	blood plasma	108:119	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
32217095	3	8	theme	real-time	499:507	arg1	measurement					509:519	continuous real-time measurement	488:519	continuous real-time measurement of catalytic activity of l-asparaginase	488:559	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	2	9	theme	components	422:431	arg1	presence					363:370	the presence	359:370	the presence of plasma proteins, amino acids, as well as other components of plasma	359:441	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	3	10	theme	l-asparaginase	546:559	arg1	activity					534:541	catalytic activity	524:541	catalytic activity of l-asparaginase	524:559	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	4	11	theme	spectroscopic	870:882	arg1	properties					884:893	the spectroscopic properties	866:893	the spectroscopic properties of substrate and product	866:918	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	0	12	theme	L-asparaginase	11:24	arg1	assay					35:39	FTIR-based L-asparaginase activity assay	0:39	FTIR-based L-asparaginase activity assay	0:39	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
32217095	4	13	theme	substrate	898:906	arg1	properties					884:893	the spectroscopic properties	866:893	the spectroscopic properties of substrate and product	866:918	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	3	14	theme	PEG-chitosan	579:590	arg1	form					603:606	PEG-chitosan conjugated form	579:606	PEG-chitosan conjugated form	579:606	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	0	15	theme	FTIR-based	0:9	arg1	assay					35:39	FTIR-based L-asparaginase activity assay	0:39	FTIR-based L-asparaginase activity assay	0:39	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
32217095	3	16	theme	conjugated	592:601	arg1	form					603:606	PEG-chitosan conjugated form	579:606	PEG-chitosan conjugated form	579:606	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	2	17	theme	acids	398:402	arg1	presence					363:370	the presence	359:370	the presence of plasma proteins, amino acids, as well as other components of plasma	359:441	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	4	18	with	measurement	940:950	arg1	absorption					957:966	absorption	957:966	absorption	957:966	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	4	18	with	measurement	940:950	arg1	spectroscopy					984:995	fluorescence spectroscopy	971:995	fluorescence spectroscopy	971:995	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	3	19	theme	FTIR	462:465	arg1	spectroscopy					467:478	FTIR spectroscopy	462:478	FTIR spectroscopy	462:478	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	2	20	theme	plasma	436:441	arg1	proteins					382:389	plasma proteins	375:389	plasma proteins	375:389	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	2	20	theme	plasma	436:441	arg1	acids					398:402	amino acids	392:402	amino acids	392:402	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	2	20	theme	plasma	436:441	arg1	components					422:431	other components	416:431	other components of plasma	416:441	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	3	21	theme	non-transparent	658:672	arg1	systems					718:724	colloidal systems	708:724	colloidal systems	708:724	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	21	theme	non-transparent	658:672	arg1	plasma					735:740	blood plasma	729:740	blood plasma	729:740	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	21	theme	non-transparent	658:672	arg1	systems					689:695	heterogeneous non-transparent multicomponent systems	644:695	heterogeneous non-transparent multicomponent systems	644:695	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	1	22	theme	Complex	122:128	arg1	micelles					161:168	micelles	161:168	micelles	161:168	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	22	theme	Complex	122:128	arg1	plasma					179:184	blood plasma	173:184	blood plasma	173:184	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	22	theme	Complex	122:128	arg1	systems					144:150	Complex heterogeneous systems	122:150	Complex heterogeneous systems	122:150	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	23	theme	challenging	212:222	arg1	environment					224:234	a particularly challenging environment	197:234	a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase	197:312	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	0	24	theme	continuous	49:58	arg1	measurements					60:71	continuous measurements	49:71	continuous measurements	49:71	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
32217095	3	25	theme	sample	762:767	arg1	preparation					769:779	no sample preparation	759:779	no sample preparation	759:779	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	26	theme	multicomponent	674:687	arg1	systems					718:724	colloidal systems	708:724	colloidal systems	708:724	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	26	theme	multicomponent	674:687	arg1	plasma					735:740	blood plasma	729:740	blood plasma	729:740	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	26	theme	multicomponent	674:687	arg1	systems					689:695	heterogeneous non-transparent multicomponent systems	644:695	heterogeneous non-transparent multicomponent systems	644:695	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	2	27	theme	other	416:420	arg1	components					422:431	other components	416:431	other components of plasma	416:441	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	4	28	theme	fluorescence	971:982	arg1	spectroscopy					984:995	fluorescence spectroscopy	971:995	fluorescence spectroscopy	971:995	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	3	29	theme	aqueous	612:618	arg1	solutions					620:628	aqueous solutions	612:628	aqueous solutions	612:628	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	4	30	theme	direct	933:938	arg1	measurement					940:950	direct measurement	933:950	direct measurement with absorption or fluorescence spectroscopy	933:995	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	3	31	theme	heterogeneous	644:656	arg1	systems					718:724	colloidal systems	708:724	colloidal systems	708:724	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	31	theme	heterogeneous	644:656	arg1	plasma					735:740	blood plasma	729:740	blood plasma	729:740	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	31	theme	heterogeneous	644:656	arg1	systems					689:695	heterogeneous non-transparent multicomponent systems	644:695	heterogeneous non-transparent multicomponent systems	644:695	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	1	32	theme	heterogeneous	130:142	arg1	micelles					161:168	micelles	161:168	micelles	161:168	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	32	theme	heterogeneous	130:142	arg1	plasma					179:184	blood plasma	173:184	blood plasma	173:184	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	32	theme	heterogeneous	130:142	arg1	systems					144:150	Complex heterogeneous systems	122:150	Complex heterogeneous systems	122:150	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	3	33	theme	catalytic	524:532	arg1	activity					534:541	catalytic activity	524:541	catalytic activity of l-asparaginase	524:559	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	3	34	theme	colloidal	708:716	arg1	systems					718:724	colloidal systems	708:724	colloidal systems	708:724	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	4	35	theme	enzymatic	840:848	arg1	reactions					850:858	other enzymatic reactions	834:858	other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy	834:995	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	3	36	theme	continuous	488:497	arg1	measurement					509:519	continuous real-time measurement	488:519	continuous real-time measurement of catalytic activity of l-asparaginase	488:559	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	0	37	theme	dense	86:90	arg1	media					92:96	optically dense media	76:96	optically dense media including blood plasma	76:119	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
32217095	0	37	theme	dense	86:90	arg1	plasma					114:119	blood plasma	108:119	blood plasma	108:119	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
32217095	4	38	theme	other	834:838	arg1	reactions					850:858	other enzymatic reactions	834:858	other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy	834:995	The approach developed is potentially applicable to other enzymatic reactions where the spectroscopic properties of substrate and product do not allow direct measurement with absorption or fluorescence spectroscopy.					
32217095	2	39	theme	amino	392:396	arg1	acids					398:402	amino acids	392:402	amino acids	392:402	Existing methods are strongly interfered by the presence of plasma proteins, amino acids, as well as other components of plasma.					
32217095	1	40	theme	catalytic	251:259	arg1	parameters					261:270	the catalytic parameters	247:270	the catalytic parameters	247:270	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	1	40	theme	catalytic	251:259	arg1	l-asparaginase					299:312	l-asparaginase	299:312	l-asparaginase	299:312	Complex heterogeneous systems, such as micelles or blood plasma, represent a particularly challenging environment to measure the catalytic parameters of some enzymes, including l-asparaginase.					
32217095	3	41	theme	blood	729:733	arg1	plasma					735:740	blood plasma	729:740	blood plasma	729:740	Here we show that FTIR spectroscopy enables continuous real-time measurement of catalytic activity of l-asparaginase, in native and in PEG-chitosan conjugated form, in aqueous solutions as well as in heterogeneous non-transparent multicomponent systems, including colloidal systems or blood plasma, with minimal or no sample preparation.					
32217095	0	42	theme	activity	26:33	arg1	assay					35:39	FTIR-based L-asparaginase activity assay	0:39	FTIR-based L-asparaginase activity assay	0:39	FTIR-based L-asparaginase activity assay enables continuous measurements in optically dense media including blood plasma.					
35006788	6	0	theme	efficient	1024:1032	arg1	healing					1040:1046	a highly efficient wound healing	1015:1046	a highly efficient wound healing dressing	1015:1055	Therefore, this study develops a highly efficient wound healing dressing, which is expected to be used directly in clinics.					
35006788	3	1	theme	water	479:483	arg1	vapor					485:489	water vapor	479:489	water vapor	479:489	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	4	2	theme	microbial	713:721	arg1	infection					723:731	microbial infection	713:731	microbial infection	713:731	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	2	3	theme	traditional	157:167	arg1	dressing					175:182	a traditional wound dressing	155:182	a traditional wound dressing	155:182	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	1	4	theme	Adequate	75:82	arg1	treatment					84:92	Adequate treatment	75:92	Adequate treatment of skin wounds	75:107	Adequate treatment of skin wounds is vital to health.					
35006788	3	5	theme	moderate	449:456	arg1	rate					471:474	a moderate transmission rate	447:474	a moderate transmission rate of water vapor	447:489	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	5	6	theme	±	877:877	arg1	%					883:883	93.03 ± 1.08%	871:883	93.03 ± 1.08%	871:883	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	3	7	theme	excellent	492:500	arg1	performance					512:522	excellent toughness performance	492:522	excellent toughness performance	492:522	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	8	theme	nanoporous	335:344	arg1	NPG					356:358	NPG	356:358	NPG	356:358	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	8	theme	nanoporous	335:344	arg1	graphene					346:353	nanoporous graphene	335:353	nanoporous graphene (NPG)	335:359	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	9	theme	toughness	502:510	arg1	performance					512:522	excellent toughness performance	492:522	excellent toughness performance	492:522	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	10	theme	composite	412:420	arg1	membrane					422:429	a composite membrane	410:429	a composite membrane	410:429	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	0	11	theme	Nanoporous	2:11	arg1	Membrane					37:44	A Nanoporous Graphene/Nitrocellulose Membrane	0:44	A Nanoporous Graphene/Nitrocellulose Membrane	0:44	A Nanoporous Graphene/Nitrocellulose Membrane Beneficial to Wound Healing.					
35006788	1	12	theme	skin	97:100	arg1	wounds					102:107	skin wounds	97:107	skin wounds	97:107	Adequate treatment of skin wounds is vital to health.					
35006788	6	13	used	used	1082:1085	arg2	dressing					1048:1055	a highly efficient wound healing dressing	1015:1055	a highly efficient wound healing dressing	1015:1055	Therefore, this study develops a highly efficient wound healing dressing, which is expected to be used directly in clinics.					
35006788	1	14	theme	wounds	102:107	arg1	treatment					84:92	Adequate treatment	75:92	Adequate treatment of skin wounds	75:107	Adequate treatment of skin wounds is vital to health.					
35006788	5	15	theme	wound	838:842	arg1	closure					844:850	wound closure	838:850	the wound closure percentage	834:861	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	5	16	theme	recovery	943:950	arg1	degree					921:926	the degree	917:926	the degree of skin tissue recovery	917:950	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	0	17	theme	Graphene/Nitrocellulose	13:35	arg1	Membrane					37:44	A Nanoporous Graphene/Nitrocellulose Membrane	0:44	A Nanoporous Graphene/Nitrocellulose Membrane	0:44	A Nanoporous Graphene/Nitrocellulose Membrane Beneficial to Wound Healing.					
35006788	3	18	theme	good	529:532	arg1	biocompatibility					534:549	good biocompatibility	529:549	good biocompatibility	529:549	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	4	19	theme	antibacterial	609:621	arg1	property					623:630	an excellent broad-spectrum antibacterial property	581:630	an excellent broad-spectrum antibacterial property	581:630	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	5	20	theme	tissue	936:941	arg1	recovery					943:950	skin tissue recovery	931:950	skin tissue recovery	931:950	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	3	21	used	used	364:367	arg2	NPG					356:358	NPG	356:358	NPG	356:358	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	21	used	used	364:367	arg2	graphene					346:353	nanoporous graphene	335:353	nanoporous graphene (NPG)	335:359	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	2	22	theme	limited	226:232	arg1	permeability					238:249	its limited air permeability	222:249	its limited air permeability	222:249	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	5	23	theme	nanoporous	789:798	arg1	membrane					824:831	the nanoporous graphene/nitrocellulose membrane	785:831	the nanoporous graphene/nitrocellulose membrane	785:831	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	5	24	theme	graphene/nitrocellulose	800:822	arg1	membrane					824:831	the nanoporous graphene/nitrocellulose membrane	785:831	the nanoporous graphene/nitrocellulose membrane	785:831	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	5	25	theme	closure	844:850	arg1	percentage					852:861	the wound closure percentage	834:861	the wound closure percentage	834:861	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	2	26	theme	wound	169:173	arg1	dressing					175:182	a traditional wound dressing	155:182	a traditional wound dressing	155:182	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	6	27	theme	healing	1040:1046	arg1	dressing					1048:1055	a highly efficient wound healing dressing	1015:1055	a highly efficient wound healing dressing	1015:1055	Therefore, this study develops a highly efficient wound healing dressing, which is expected to be used directly in clinics.					
35006788	3	28	theme	transmission	458:469	arg1	rate					471:474	a moderate transmission rate	447:474	a moderate transmission rate of water vapor	447:489	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	0	29	theme	Wound	60:64	arg1	Healing					66:72	Wound Healing	60:72	Wound Healing	60:72	A Nanoporous Graphene/Nitrocellulose Membrane Beneficial to Wound Healing.					
35006788	4	30	dep	%	636:636	arg1	%					660:660	>90%	657:660	>90%	657:660	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	4	30	dep	%	636:636	arg1	aureus					678:683	Staphylococcus aureus	663:683	Staphylococcus aureus	663:683	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	4	30	dep	%	636:636	arg1	coli					651:654	Escherichia coli	639:654	Escherichia coli	639:654	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	2	31	theme	wound	203:207	arg1	healing					209:215	wound healing	203:215	wound healing	203:215	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	2	32	theme	poor	255:258	arg1	sterilization					260:272	poor sterilization	255:272	poor sterilization	255:272	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	4	33	theme	infection	723:731	arg1	risk					705:708	the risk	701:708	the risk of microbial infection for the body after trauma	701:757	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	2	34	theme	actual	312:317	arg1	efficacy					319:326	the actual efficacy	308:326	the actual efficacy	308:326	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	2	35	used	used	194:197	arg2	bandage					144:150	Nitrocellulose bandage	129:150	Nitrocellulose bandage as a traditional wound dressing	129:182	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	4	36	dep	shows	575:579	arg1	%					636:636	>98%	633:636	>98%	633:636	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	5	37	theme	skin	931:934	arg1	recovery					943:950	skin tissue recovery	931:950	skin tissue recovery	931:950	Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly.					
35006788	3	38	theme	vapor	485:489	arg1	performance					512:522	excellent toughness performance	492:522	excellent toughness performance	492:522	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	38	theme	vapor	485:489	arg1	rate					471:474	a moderate transmission rate	447:474	a moderate transmission rate of water vapor	447:489	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	3	38	theme	vapor	485:489	arg1	biocompatibility					534:549	good biocompatibility	529:549	good biocompatibility	529:549	Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility.					
35006788	4	39	theme	broad-spectrum	594:607	arg1	property					623:630	an excellent broad-spectrum antibacterial property	581:630	an excellent broad-spectrum antibacterial property	581:630	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
35006788	2	40	theme	Nitrocellulose	129:142	arg1	bandage					144:150	Nitrocellulose bandage	129:150	Nitrocellulose bandage as a traditional wound dressing	129:182	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	2	41	theme	air	234:236	arg1	permeability					238:249	its limited air permeability	222:249	its limited air permeability	222:249	Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy.					
35006788	6	42	theme	wound	1034:1038	arg1	healing					1040:1046	a highly efficient wound healing	1015:1046	a highly efficient wound healing dressing	1015:1055	Therefore, this study develops a highly efficient wound healing dressing, which is expected to be used directly in clinics.					
35006788	4	43	theme	excellent	584:592	arg1	property					623:630	an excellent broad-spectrum antibacterial property	581:630	an excellent broad-spectrum antibacterial property	581:630	Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma.					
32122483	0	0	theme	cellulose	70:78	arg1	films					101:105	carboxymethyl cellulose based food packaging films	56:105	carboxymethyl cellulose based food packaging films	56:105	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	1	1	theme	based	138:142	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	4	2	from	%	617:617	arg1	%					578:578	77.51-52.91 %	566:578	77.51-52.91 %	566:578	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	4	2	from	%	617:617	arg1	degree					590:595	swelling degree	581:595	swelling degree from 55.74 to 40.37 %	581:617	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	4	2	from	%	617:617	arg1	permeability					636:647	water vapor permeability	624:647	water vapor permeability	624:647	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	0	3	theme	carboxymethyl	56:68	arg1	films					101:105	carboxymethyl cellulose based food packaging films	56:105	carboxymethyl cellulose based food packaging films	56:105	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	1	4	theme	novel	144:148	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	0	5	theme	food	86:89	arg1	films					101:105	carboxymethyl cellulose based food packaging films	56:105	carboxymethyl cellulose based food packaging films	56:105	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	4	6	theme	water	544:548	arg1	solubility					550:559	water solubility	544:559	water solubility	544:559	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	0	7	theme	based	80:84	arg1	films					101:105	carboxymethyl cellulose based food packaging films	56:105	carboxymethyl cellulose based food packaging films	56:105	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	4	8	theme	vapor	630:634	arg1	permeability					636:647	water vapor permeability	624:647	water vapor permeability	624:647	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	7	9	theme	food	1071:1074	arg1	industry					1076:1083	the food industry	1067:1083	the food industry	1067:1083	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	4	10	dep	1.17	662:665	arg1	to					659:660	to	659:660	to	659:660	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	5	11	theme	films	752:756	arg1	ability					733:739	ABTS radical scavenging ability	709:739	ABTS radical scavenging ability	709:739	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	5	11	theme	films	752:756	arg1	DPPH					700:703	DPPH	700:703	DPPH	700:703	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	4	12	dep	40.37	611:615	arg1	to					608:609	to	608:609	to	608:609	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	1	13	theme	functional	150:159	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	0	14	theme	packaging	91:99	arg1	films					101:105	carboxymethyl cellulose based food packaging films	56:105	carboxymethyl cellulose based food packaging films	56:105	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	1	15	from	%	252:252	arg1	w/w					257:259	w/w	257:259	w/w	257:259	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	4	16	from	1.17	662:665	arg1	gm-1s-1 Pa-1					673:684	10-10 gm-1s-1 Pa-1	667:684	10-10 gm-1s-1 Pa-1	667:684	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	4	17	from	degree	590:595	arg1	gm-1s-1 Pa-1					673:684	10-10 gm-1s-1 Pa-1	667:684	10-10 gm-1s-1 Pa-1	667:684	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	2	18	theme	extract	327:333	arg1	concentration					335:347	higher extract concentration	320:347	higher extract concentration	320:347	It was revealed by SEM that higher extract concentration triggered the formation of agglomerates within the film.					
32122483	5	19	theme	radical	714:720	arg1	ability					733:739	ABTS radical scavenging ability	709:739	ABTS radical scavenging ability	709:739	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	4	20	from	decrease	532:539	arg1	solubility					550:559	water solubility	544:559	water solubility	544:559	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	7	21	theme	antimicrobial	959:971	arg1	activity					973:980	good antioxidant and antimicrobial activity	938:980	good antioxidant and antimicrobial activity	938:980	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	2	22	theme	higher	320:325	arg1	concentration					335:347	higher extract concentration	320:347	higher extract concentration	320:347	It was revealed by SEM that higher extract concentration triggered the formation of agglomerates within the film.					
32122483	5	23	theme	scavenging	722:731	arg1	ability					733:739	ABTS radical scavenging ability	709:739	ABTS radical scavenging ability	709:739	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	6	24	theme	%	870:870	arg1	biodegradability					844:859	the highest biodegradability	832:859	the highest biodegradability of 58.14 %	832:870	CMC-CRE5 film showed the highest biodegradability of 58.14 %.					
32122483	1	25	theme	Chinese	178:184	arg1	CRE					207:209	CRE	207:209	CRE	207:209	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	1	25	theme	Chinese	178:184	arg1	extract					198:204	Chinese chives root extract	178:204	Chinese chives root extract (CRE)	178:210	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	0	26	theme	chives	18:23	arg1	Effect					0:5	Effect	0:5	Effect of Chinese chives (Allium tuberosum)	0:42	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	7	27	theme	antioxidant	943:953	arg1	activity					973:980	good antioxidant and antimicrobial activity	938:980	good antioxidant and antimicrobial activity	938:980	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	4	28	from	%	578:578	arg1	gm-1s-1 Pa-1					673:684	10-10 gm-1s-1 Pa-1	667:684	10-10 gm-1s-1 Pa-1	667:684	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	1	29	theme	chives	186:191	arg1	CRE					207:209	CRE	207:209	CRE	207:209	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	1	29	theme	chives	186:191	arg1	extract					198:204	Chinese chives root extract	178:204	Chinese chives root extract (CRE)	178:210	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	0	30	theme	Chinese	10:16	arg1	Allium					26:31	Allium	26:31	Allium	26:31	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	0	30	theme	Chinese	10:16	arg1	chives					18:23	Chinese chives	10:23	Chinese chives (Allium tuberosum)	10:42	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	1	31	theme	root	193:196	arg1	CRE					207:209	CRE	207:209	CRE	207:209	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	1	31	theme	root	193:196	arg1	extract					198:204	Chinese chives root extract	178:204	Chinese chives root extract (CRE)	178:210	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	7	32	theme	bio-composite	1025:1037	arg1	material					1054:1061	a bio-composite food packaging material	1023:1061	a bio-composite food packaging material for the food industry	1023:1083	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	7	32	theme	bio-composite	1025:1037	arg1	it					998:999	it	998:999	it	998:999	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	1	33	contain	containing	167:176	arg2	CRE					207:209	CRE	207:209	CRE	207:209	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	1	33	contain	containing	167:176	arg2	extract					198:204	Chinese chives root extract	178:204	Chinese chives root extract (CRE)	178:210	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	1	33	contain	containing	167:176	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	7	34	theme	food	1039:1042	arg1	material					1054:1061	a bio-composite food packaging material	1023:1061	a bio-composite food packaging material for the food industry	1023:1083	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	7	34	theme	food	1039:1042	arg1	it					998:999	it	998:999	it	998:999	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	6	35	theme	highest	836:842	arg1	biodegradability					844:859	the highest biodegradability	832:859	the highest biodegradability of 58.14 %	832:870	CMC-CRE5 film showed the highest biodegradability of 58.14 %.					
32122483	7	36	theme	good	938:941	arg1	activity					973:980	good antioxidant and antimicrobial activity	938:980	good antioxidant and antimicrobial activity	938:980	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	4	37	dep	84 μm	519:523	arg1	to					516:517	to	516:517	to	516:517	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	5	38	dep	58	782:783	arg1	to					779:780	to	779:780	to	779:780	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	3	39	theme	Tensile	406:412	arg1	strength					414:421	Tensile strength	406:421	Tensile strength of the films	406:434	Tensile strength of the films was decreased from 30.91 to 16.48 MPa.					
32122483	0	40	dep	Allium	26:31	arg1	tuberosum					33:41	Allium tuberosum	26:41	Allium tuberosum	26:41	Effect of Chinese chives (Allium tuberosum) addition to carboxymethyl cellulose based food packaging films.					
32122483	4	41	theme	water	624:628	arg1	permeability					636:647	water vapor permeability	624:647	water vapor permeability	624:647	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	5	42	theme	ABTS	709:712	arg1	ability					733:739	ABTS radical scavenging ability	709:739	ABTS radical scavenging ability	709:739	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	4	43	theme	films	488:492	arg1	Thickness					475:483	Thickness	475:483	Thickness of films	475:492	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	1	44	theme	Carboxymethyl	108:120	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	4	45	from	permeability	636:647	arg1	gm-1s-1 Pa-1					673:684	10-10 gm-1s-1 Pa-1	667:684	10-10 gm-1s-1 Pa-1	667:684	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	7	46	theme	packaging	1044:1052	arg1	material					1054:1061	a bio-composite food packaging material	1023:1061	a bio-composite food packaging material for the food industry	1023:1083	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	7	46	theme	packaging	1044:1052	arg1	it					998:999	it	998:999	it	998:999	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	7	47	theme	CRE	910:912	arg1	addition					898:905	the addition	894:905	the addition of CRE into CMC	894:921	The film prepared by the addition of CRE into CMC also exhibited good antioxidant and antimicrobial activity indicating that it could be developed as a bio-composite food packaging material for the food industry.					
32122483	1	48	theme	cellulose	122:130	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	1	49	dep	concentrations	225:238	arg1	%					252:252	1, 3 and 5 %	241:252	1, 3 and 5 %	241:252	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	3	50	theme	films	430:434	arg1	strength					414:421	Tensile strength	406:421	Tensile strength of the films	406:434	Tensile strength of the films was decreased from 30.91 to 16.48 MPa.					
32122483	5	51	theme	CMC-CRE	744:750	arg1	films					752:756	CMC-CRE films	744:756	CMC-CRE films	744:756	DPPH and ABTS radical scavenging ability of CMC-CRE films was increased from 0 to 58 % and 82 %, respectively.					
32122483	1	52	theme	CMC	133:135	arg1	films					161:165	Carboxymethyl cellulose (CMC) based novel functional films	108:165	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w)	108:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
32122483	4	53	theme	swelling	581:588	arg1	degree					590:595	swelling degree	581:595	swelling degree from 55.74 to 40.37 %	581:617	Thickness of films was increased from 43 to 84 μm, while decrease in water solubility from 77.51-52.91 %, swelling degree from 55.74 to 40.37 %, and water vapor permeability from 5.76 to 1.17 10-10 gm-1s-1 Pa-1 was observed.					
32122483	6	54	theme	CMC-CRE5	811:818	arg1	film					820:823	CMC-CRE5 film	811:823	CMC-CRE5 film	811:823	CMC-CRE5 film showed the highest biodegradability of 58.14 %.					
32122483	2	55	theme	agglomerates	376:387	arg1	formation					363:371	the formation	359:371	the formation of agglomerates	359:387	It was revealed by SEM that higher extract concentration triggered the formation of agglomerates within the film.					
32122483	1	56	theme	different	215:223	arg1	concentrations					225:238	different concentrations	215:238	different concentrations (1, 3 and 5 % in w/w)	215:260	Carboxymethyl cellulose (CMC) based novel functional films containing Chinese chives root extract (CRE) at different concentrations (1, 3 and 5 % in w/w) were successfully fabricated.					
33091776	9	0	theme	146	1465:1467	arg1	capacity					1453:1460	a capacity	1451:1460	a capacity of 146 and 193 mg/g respectively	1451:1493	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	1	theme	193 mg/g	1473:1480	arg1	capacity					1453:1460	a capacity	1451:1460	a capacity of 146 and 193 mg/g respectively	1451:1493	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	7	2	from	aerogel	1114:1120	arg1	desorption					1094:1103	Pb2+ desorption	1089:1103	Pb2+ desorption from the aerogel	1089:1120	A third series of experiments explored Pb2+ desorption from the aerogel at low pH and in highly acidic conditions.					
33091776	9	3	theme	toxic	1406:1410	arg1	Cu2+					1441:1444	Cu2+	1441:1444	Cu2+	1441:1444	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	3	theme	toxic	1406:1410	arg1	salts					1418:1422	other toxic metal salts	1400:1422	other toxic metal salts such as La3+ and Cu2+	1400:1444	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	3	theme	toxic	1406:1410	arg1	La3+					1432:1435	La3+	1432:1435	La3+	1432:1435	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	3	4	theme	materials	496:504	arg1	sol-gel					471:477	a sol-gel	469:477	a sol-gel of the composite materials followed by a freeze drying method	469:539	Aerogels were made by a sol-gel of the composite materials followed by a freeze drying method.					
33091776	3	5	theme	freeze	520:525	arg1	method					534:539	a freeze drying method	518:539	a freeze drying method	518:539	Aerogels were made by a sol-gel of the composite materials followed by a freeze drying method.					
33091776	5	6	theme	starting	792:799	arg1	concentration					801:813	a starting concentration 0.48 mM	790:821	a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls	790:893	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	5	6	theme	starting	792:799	arg1	100 mg/l					824:831	100 mg/l	824:831	100 mg/l	824:831	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	4	7	theme	50 mm	573:577	arg1	disks					588:592	50 mm diameter disks	573:592	50 mm diameter disks	573:592	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	1	8	from	impacts	260:266	arg1	health					277:282	human health	271:282	human health	271:282	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	1	9	theme	toxic	192:196	arg1	metals					198:203	toxic metals	192:203	toxic metals that exceed the recommended international limits	192:252	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	8	10	theme	mean	1294:1297	arg1	capacity					1318:1325	an ultra-high mean maximum adsorption capacity	1280:1325	an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5	1280:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	5	11	theme	adsorption	767:776	arg1	method					778:783	a batch adsorption method	759:783	a batch adsorption method	759:783	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	8	12	theme	adsorption	1307:1316	arg1	capacity					1318:1325	an ultra-high mean maximum adsorption capacity	1280:1325	an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5	1280:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	5	13	with	n =	875:877	arg1	controls					886:893	controls	886:893	controls	886:893	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	11	14	theme	proton	1801:1806	arg1	tunnelling					1808:1817	proton tunnelling	1801:1817	proton tunnelling	1801:1817	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	6	15	theme	experiments	915:925	arg1	series					905:910	A second series	896:910	A second series of experiments	896:925	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	8	16	theme	filter	1177:1182	arg1	system					1184:1189	This simple filter system	1165:1189	This simple filter system	1165:1189	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	0	17	theme	lead	54:57	arg1	removal					43:49	the removal	39:49	the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade	39:126	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	4	18	theme	open	629:632	arg1	network					641:647	an open porous network	626:647	an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration	626:717	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	0	19	theme	drinking	64:71	arg1	water					73:77	drinking water	64:77	drinking water using a novel nano-enhanced ion exchange cascade	64:126	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	1	20	theme	developing	146:155	arg1	countries					157:165	developing countries	146:165	developing countries	146:165	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	6	21	dep	valencies	992:1000	arg1	Na+					1003:1005	Na+	1003:1005	Na+	1003:1005	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	6	21	dep	valencies	992:1000	arg1	La3+					1020:1023	La3+	1020:1023	La3+	1020:1023	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	6	21	dep	valencies	992:1000	arg1	Cu2+					1014:1017	Cu2+	1014:1017	Cu2+	1014:1017	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	6	21	dep	valencies	992:1000	arg1	Ca2+					1008:1011	Ca2+	1008:1011	Ca2+	1008:1011	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	7	22	theme	experiments	1068:1078	arg1	series					1058:1063	A third series	1050:1063	A third series of experiments	1050:1078	A third series of experiments explored Pb2+ desorption from the aerogel at low pH and in highly acidic conditions.					
33091776	5	23	from	concentration	801:813	arg1	water					855:859	ultrapure water	845:859	ultrapure water over 240 min, n = 4 with controls	845:893	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	0	24	dep	novel	87:91	arg1	nano-enhanced					93:105	nano-enhanced	93:105	nano-enhanced	93:105	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	8	25	theme	batch	1203:1207	arg1	methodology					1220:1230	a batch adsorption methodology	1201:1230	a batch adsorption methodology	1201:1230	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	2	26	theme	graphene	365:372	arg1	oxide					374:378	graphene oxide	365:378	graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water	365:444	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	0	27	theme	novel	87:91	arg1	cascade					120:126	a novel nano-enhanced ion exchange cascade	85:126	a novel nano-enhanced ion exchange cascade	85:126	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	9	28	theme	other	1400:1404	arg1	Cu2+					1441:1444	Cu2+	1441:1444	Cu2+	1441:1444	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	28	theme	other	1400:1404	arg1	salts					1418:1422	other toxic metal salts	1400:1422	other toxic metal salts such as La3+ and Cu2+	1400:1444	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	28	theme	other	1400:1404	arg1	La3+					1432:1435	La3+	1432:1435	La3+	1432:1435	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	0	29	theme	Graphene	0:7	arg1	biopolymer					15:24	Graphene oxide biopolymer	0:24	Graphene oxide biopolymer	0:24	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	9	30	theme	metal	1412:1416	arg1	Cu2+					1441:1444	Cu2+	1441:1444	Cu2+	1441:1444	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	30	theme	metal	1412:1416	arg1	salts					1418:1422	other toxic metal salts	1400:1422	other toxic metal salts such as La3+ and Cu2+	1400:1444	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	9	30	theme	metal	1412:1416	arg1	La3+					1432:1435	La3+	1432:1435	La3+	1432:1435	The aerogel can also adsorb other toxic metal salts such as La3+ and Cu2+ with a capacity of 146 and 193 mg/g respectively.					
33091776	6	31	theme	competing	964:972	arg1	ions					974:977	different competing ions	954:977	different competing ions	954:977	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	11	32	theme	primary	1723:1729	arg1	mechanism					1731:1739	the primary mechanism	1719:1739	the primary mechanism involved is ion exchange	1719:1764	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	2	33	theme	potable	432:438	arg1	water					440:444	potable water	432:444	potable water	432:444	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	1	34	contain	contains	173:180	arg1	water					137:141	Potable water	129:141	Potable water in developing countries	129:165	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	1	34	contain	contains	173:180	arg2	levels					182:187	levels	182:187	levels of toxic metals that exceed the recommended international limits	182:252	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	11	35	theme	alginate	1648:1655	arg1	aerogels					1657:1664	GO alginate aerogels	1645:1664	GO alginate aerogels	1645:1664	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	2	36	dep	oxide	374:378	arg1	GO					381:382	GO	381:382	GO	381:382	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	2	37	theme	study	308:312	arg1	aim					289:291	The aim	285:291	The aim of the present study	285:312	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	4	38	dep	deep	600:603	arg1	disks					588:592	50 mm diameter disks	573:592	50 mm diameter disks	573:592	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	8	39	theme	ultra-high	1283:1292	arg1	capacity					1318:1325	an ultra-high mean maximum adsorption capacity	1280:1325	an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5	1280:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	6	40	from	valencies	992:1000	arg1	adsorption					940:949	the adsorption	936:949	the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+)	936:1024	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	2	41	theme	cost	335:338	arg1	aerogel					340:346	a low cost aerogel	329:346	a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water	329:444	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	1	42	theme	recommended	221:231	arg1	limits					247:252	the recommended international limits	217:252	the recommended international limits	217:252	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	7	43	theme	low	1125:1127	arg1	pH					1129:1130	low pH	1125:1130	low pH	1125:1130	A third series of experiments explored Pb2+ desorption from the aerogel at low pH and in highly acidic conditions.					
33091776	3	44	theme	composite	486:494	arg1	materials					496:504	the composite materials	482:504	the composite materials followed by a freeze drying method	482:539	Aerogels were made by a sol-gel of the composite materials followed by a freeze drying method.					
33091776	11	45	theme	Pb2+	1896:1899	arg1	remediation					1901:1911	Pb2+ remediation	1896:1911	Pb2+ remediation	1896:1911	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	6	46	theme	equivalent	1032:1041	arg1	media					1043:1047	an equivalent media	1029:1047	an equivalent media	1029:1047	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	10	47	theme	Pb2+	1570:1573	arg1	%					1561:1561	98%	1559:1561	98% of the Pb2+	1559:1573	Furthermore, the aerogel structure can be acid washed removing 98% of the Pb2+ from the structure within three minutes.					
33091776	10	47	theme	Pb2+	1570:1573	arg1	Pb2+					1570:1573	the Pb2+	1566:1573	the Pb2+	1566:1573	Furthermore, the aerogel structure can be acid washed removing 98% of the Pb2+ from the structure within three minutes.					
33091776	7	48	theme	Pb2+	1089:1092	arg1	desorption					1094:1103	Pb2+ desorption	1089:1103	Pb2+ desorption from the aerogel	1089:1120	A third series of experiments explored Pb2+ desorption from the aerogel at low pH and in highly acidic conditions.					
33091776	0	49	theme	exchange	111:118	arg1	cascade					120:126	a novel nano-enhanced ion exchange cascade	85:126	a novel nano-enhanced ion exchange cascade	85:126	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	11	50	theme	contributing	1828:1839	arg1	factor					1841:1846	a contributing factor	1826:1846	a contributing factor	1826:1846	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	11	50	theme	contributing	1828:1839	arg1	phenomenon					1782:1791	other phenomenon	1776:1791	other phenomenon such as proton tunnelling	1776:1817	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	11	51	theme	ultra-high	1855:1864	arg1	efficiency					1866:1875	the ultra-high efficiency	1851:1875	the ultra-high efficiency of the aerogel for Pb2+ remediation	1851:1911	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	8	52	theme	maximum	1299:1305	arg1	capacity					1318:1325	an ultra-high mean maximum adsorption capacity	1280:1325	an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5	1280:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	11	53	theme	ion	1753:1755	arg1	exchange					1757:1764	ion exchange	1753:1764	ion exchange	1753:1764	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	5	54	theme	batch	761:765	arg1	method					778:783	a batch adsorption method	759:783	a batch adsorption method	759:783	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	4	55	theme	diameter	579:586	arg1	disks					588:592	50 mm diameter disks	573:592	50 mm diameter disks	573:592	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	3	56	theme	drying	527:532	arg1	method					534:539	a freeze drying method	518:539	a freeze drying method	518:539	Aerogels were made by a sol-gel of the composite materials followed by a freeze drying method.					
33091776	11	57	theme	aerogel	1884:1890	arg1	efficiency					1866:1875	the ultra-high efficiency	1851:1875	the ultra-high efficiency of the aerogel for Pb2+ remediation	1851:1911	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	8	58	theme	504 mg/g	1330:1337	arg1	capacity					1318:1325	an ultra-high mean maximum adsorption capacity	1280:1325	an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5	1280:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	4	59	theme	porous	634:639	arg1	network					641:647	an open porous network	626:647	an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration	626:717	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	11	60	theme	other	1776:1780	arg1	factor					1841:1846	a contributing factor	1826:1846	a contributing factor	1826:1846	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	11	60	theme	other	1776:1780	arg1	phenomenon					1782:1791	other phenomenon	1776:1791	other phenomenon such as proton tunnelling	1776:1817	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	5	61	theme	Pb2+	837:840	arg1	concentration					801:813	a starting concentration 0.48 mM	790:821	a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls	790:893	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	5	61	theme	Pb2+	837:840	arg1	100 mg/l					824:831	100 mg/l	824:831	100 mg/l	824:831	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	8	62	theme	Pb2+	1342:1345	arg1	504 mg/g					1330:1337	504 mg/g	1330:1337	504 mg/g of Pb2+ within 240 mins at pH 5	1330:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	1	63	theme	Potable	129:135	arg1	water					137:141	Potable water	129:141	Potable water in developing countries	129:165	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	8	64	theme	simple	1170:1175	arg1	system					1184:1189	This simple filter system	1165:1189	This simple filter system	1165:1189	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	6	65	theme	second	898:903	arg1	series					905:910	A second series	896:910	A second series of experiments	896:925	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	0	66	theme	ion	107:109	arg1	cascade					120:126	a novel nano-enhanced ion exchange cascade	85:126	a novel nano-enhanced ion exchange cascade	85:126	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	0	67	from	water	73:77	arg1	removal					43:49	the removal	39:49	the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade	39:126	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	2	68	theme	present	300:306	arg1	study					308:312	the present study	296:312	the present study	296:312	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	7	69	theme	third	1052:1056	arg1	series					1058:1063	A third series	1050:1063	A third series of experiments	1050:1078	A third series of experiments explored Pb2+ desorption from the aerogel at low pH and in highly acidic conditions.					
33091776	1	70	from	water	137:141	arg1	countries					157:165	developing countries	146:165	developing countries	146:165	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	6	71	theme	different	982:990	arg1	valencies					992:1000	different valencies	982:1000	different valencies (Na+, Ca2+, Cu2+, La3+)	982:1024	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	0	72	theme	oxide	9:13	arg1	biopolymer					15:24	Graphene oxide biopolymer	0:24	Graphene oxide biopolymer	0:24	Graphene oxide biopolymer aerogels for the removal of lead from drinking water using a novel nano-enhanced ion exchange cascade.					
33091776	5	73	theme	ultrapure	845:853	arg1	water					855:859	ultrapure water	845:859	ultrapure water over 240 min, n = 4 with controls	845:893	Firstly, the study was conducted using a batch adsorption method from a starting concentration 0.48 mM (100 mg/l) of Pb2+ in ultrapure water over 240 min, n = 4 with controls.					
33091776	6	74	theme	ions	974:977	arg1	adsorption					940:949	the adsorption	936:949	the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+)	936:1024	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	8	75	theme	adsorption	1209:1218	arg1	methodology					1220:1230	a batch adsorption methodology	1201:1230	a batch adsorption methodology	1201:1230	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	1	76	theme	human	271:275	arg1	health					277:282	human health	271:282	human health	271:282	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	6	77	theme	different	954:962	arg1	ions					974:977	different competing ions	954:977	different competing ions	954:977	A second series of experiments compared the adsorption of different competing ions at different valencies (Na+, Ca2+, Cu2+, La3+) in an equivalent media.					
33091776	4	78	theme	micrometres	662:672	arg1	network					641:647	an open porous network	626:647	an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration	626:717	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	1	79	theme	metals	198:203	arg1	levels					182:187	levels	182:187	levels of toxic metals that exceed the recommended international limits	182:252	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
33091776	7	80	theme	acidic	1146:1151	arg1	conditions					1153:1162	highly acidic conditions	1139:1162	highly acidic conditions	1139:1162	A third series of experiments explored Pb2+ desorption from the aerogel at low pH and in highly acidic conditions.					
33091776	8	81	theme	high	1244:1247	arg1	affinity					1249:1256	a high affinity	1242:1256	a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5	1242:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	2	82	theme	low	331:333	arg1	aerogel					340:346	a low cost aerogel	329:346	a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water	329:444	The aim of the present study was to develop a low cost aerogel synthesised from graphene oxide (GO) cross-linked with alginate to remove Pb2+ from potable water.					
33091776	4	83	dep	150	658:660	arg1	to					655:656	to	655:656	to	655:656	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	11	84	dep	aerogels	1657:1664	arg1	GO					1645:1646	GO	1645:1646	GO	1645:1646	Overall, the data shows that GO alginate aerogels are highly effective at removing Pb2+ from water and the primary mechanism involved is ion exchange, although other phenomenon such as proton tunnelling may be a contributing factor to the ultra-high efficiency of the aerogel for Pb2+ remediation.					
33091776	10	85	theme	aerogel	1513:1519	arg1	structure					1521:1529	the aerogel structure	1509:1529	the aerogel structure	1509:1529	Furthermore, the aerogel structure can be acid washed removing 98% of the Pb2+ from the structure within three minutes.					
33091776	10	85	theme	aerogel	1513:1519	arg1	acid					1538:1541	acid	1538:1541	acid washed removing 98% of the Pb2+ from the structure within three minutes	1538:1613	Furthermore, the aerogel structure can be acid washed removing 98% of the Pb2+ from the structure within three minutes.					
33091776	4	86	theme	aerogels	559:566	arg1	shape					546:550	The shape	542:550	The shape of the aerogels	542:566	The shape of the aerogels were 50 mm diameter disks, 5 mm deep and characterised by an open porous network of 50 to 150 micrometres which are mechanically robust upon hydration.					
33091776	8	87	from	pH	1366:1367	arg1	240 mins					1354:1361	240 mins	1354:1361	240 mins at pH 5	1354:1369	This simple filter system, based on a batch adsorption methodology expresses a high affinity for Pb2+ resulting in an ultra-high mean maximum adsorption capacity of 504 mg/g of Pb2+ within 240 mins at pH 5.					
33091776	1	88	theme	international	233:245	arg1	limits					247:252	the recommended international limits	217:252	the recommended international limits	217:252	Potable water in developing countries often contains levels of toxic metals that exceed the recommended international limits, with impacts on human health.					
32661565	2	0	theme	2.0	513:515	arg1	%					516:516	%	516:516	%	516:516	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	4	1	theme	cells	712:716	arg1	271.48					723:728	271.48	723:728	271.48	723:728	The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively.					
32661565	4	1	theme	cells	712:716	arg1	half-lives					676:685	The half-lives	672:685	The half-lives of composite immobilized cells	672:716	The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively.					
32661565	6	2	theme	3-cyanopyridine	996:1010	arg1	batches					985:991	14 batches	982:991	14 batches of 3-cyanopyridine with feeding concentration of 250 mM	982:1047	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	4	3	theme	immobilized	700:710	arg1	cells					712:716	composite immobilized cells	690:716	composite immobilized cells	690:716	The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively.					
32661565	1	4	theme	immobilized	257:267	arg1	cells					269:273	sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells	214:273	sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells	214:273	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	1	5	dep	putida	290:295	arg1	mut-D3					297:302	mut-D3	297:302	Pseudomonas putida mut-D3 harboring nitrilase	278:322	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	3	6	theme	optimal	545:551	arg1	pH					553:554	optimal pH	545:554	optimal pH	545:554	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	1	7	theme	cells	269:273	arg1	requirements					198:209	industrial application requirements	175:209	industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase	175:322	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	0	8	theme	silica	99:104	arg1	addition					87:94	addition	87:94	addition of silica and calcium carbonate	87:126	Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.					
32661565	6	9	theme	SA-PVA	1101:1106	arg1	cells					1120:1124	the SA-PVA immobilized cells	1097:1124	the SA-PVA immobilized cells	1097:1124	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	3	10	dep	pH	553:554	arg1	The					541:543	The	541:543	The	541:543	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	2	11	theme	0.6	529:531	arg1	%					532:532	%	532:532	%	532:532	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	4	12	theme	immobilized	822:832	arg1	cells					834:838	SA-PVA immobilized cells	815:838	SA-PVA immobilized cells	815:838	The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively.					
32661565	0	13	theme	calcium	110:116	arg1	carbonate					118:126	calcium carbonate	110:126	calcium carbonate	110:126	Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.					
32661565	4	14	theme	SA-PVA	815:820	arg1	cells					834:838	SA-PVA immobilized cells	815:838	SA-PVA immobilized cells	815:838	The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively.					
32661565	0	15	theme	carbonate	118:126	arg1	addition					87:94	addition	87:94	addition of silica and calcium carbonate	87:126	Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.					
32661565	2	16	theme	inorganic	472:480	arg1	materials					482:490	inorganic materials	472:490	inorganic materials	472:490	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	6	17	theme	immobilized	950:960	arg1	cells					962:966	The composite immobilized cells	936:966	The composite immobilized cells	936:966	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	3	18	theme	SA-PVA	576:581	arg1	cells					595:599	SA-PVA immobilized cells	576:599	SA-PVA immobilized cells	576:599	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	0	19	theme	cells	57:61	arg1	performance					27:37	the biocatalytic performance	10:37	the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate	10:126	Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.					
32661565	1	20	theme	industrial	175:184	arg1	requirements					198:209	industrial application requirements	175:209	industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase	175:322	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	1	21	theme	putida	290:295	arg1	requirements					198:209	industrial application requirements	175:209	industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase	175:322	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	1	22	theme	application	186:196	arg1	requirements					198:209	industrial application requirements	175:209	industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase	175:322	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	0	23	theme	biocatalytic	14:25	arg1	performance					27:37	the biocatalytic performance	10:37	the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate	10:126	Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.					
32661565	5	24	theme	composite	884:892	arg1	cells					906:910	the composite immobilized cells	880:910	the composite immobilized cells	880:910	The storage stability of the composite immobilized cells was slightly increased.					
32661565	4	25	theme	composite	690:698	arg1	cells					712:716	composite immobilized cells	690:716	composite immobilized cells	690:716	The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively.					
32661565	0	26	theme	co-immobilized	42:55	arg1	cells					57:61	co-immobilized cells	42:61	co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate	42:126	Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.					
32661565	6	27	theme	nicotinic	1148:1156	arg1	acid					1158:1161	346 g L-1 nicotinic acid	1138:1161	346 g L-1 nicotinic acid	1138:1161	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	1	28	theme	mechanical	399:408	arg1	strength					410:417	mechanical strength	399:417	mechanical strength	399:417	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	5	29	theme	storage	859:865	arg1	stability					867:875	The storage stability	855:875	The storage stability of the composite immobilized cells	855:910	The storage stability of the composite immobilized cells was slightly increased.					
32661565	6	30	theme	composite	940:948	arg1	cells					962:966	The composite immobilized cells	936:966	The composite immobilized cells	936:966	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	3	31	theme	composite	605:613	arg1	cells					627:631	composite immobilized cells	605:631	composite immobilized cells	605:631	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	6	32	theme	250 mM	1042:1047	arg1	concentration					1025:1037	feeding concentration	1017:1037	feeding concentration of 250 mM	1017:1047	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	3	33	theme	immobilized	583:593	arg1	cells					595:599	SA-PVA immobilized cells	576:599	SA-PVA immobilized cells	576:599	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	5	34	theme	immobilized	894:904	arg1	cells					906:910	the composite immobilized cells	880:910	the composite immobilized cells	880:910	The storage stability of the composite immobilized cells was slightly increased.					
32661565	2	35	theme	materials	482:490	arg1	silica					518:523	2.0% silica	513:523	2.0% silica	513:523	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	2	35	theme	materials	482:490	arg1	concentrations					454:467	The concentrations	450:467	The concentrations of inorganic materials	450:490	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	1	36	theme	inorganic	325:333	arg1	materials					335:343	inorganic materials	325:343	inorganic materials	325:343	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	1	37	theme	mass	423:426	arg1	performance					437:447	mass transfer performance	423:447	mass transfer performance	423:447	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	5	38	theme	cells	906:910	arg1	stability					867:875	The storage stability	855:875	The storage stability of the composite immobilized cells	855:910	The storage stability of the composite immobilized cells was slightly increased.					
32661565	1	39	theme	transfer	428:435	arg1	performance					437:447	mass transfer performance	423:447	mass transfer performance	423:447	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	6	40	with	batches	985:991	arg1	concentration					1025:1037	feeding concentration	1017:1037	feeding concentration of 250 mM	1017:1047	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	6	41	theme	346 g L-1	1138:1146	arg1	acid					1158:1161	346 g L-1 nicotinic acid	1138:1161	346 g L-1 nicotinic acid	1138:1161	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	3	42	dep	8.0	643:645	arg1	both					638:641	both	638:641	both	638:641	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	6	43	theme	nicotinic	1075:1083	arg1	acid					1085:1088	418 g ·L-1 nicotinic acid	1064:1088	418 g ·L-1 nicotinic acid	1064:1088	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	1	44	theme	nicotinic	140:148	arg1	NA					156:157	NA	156:157	NA	156:157	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	1	44	theme	nicotinic	140:148	arg1	acid					150:153	nicotinic acid	140:153	nicotinic acid (NA) yield	140:164	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	3	45	theme	immobilized	615:625	arg1	cells					627:631	composite immobilized cells	605:631	composite immobilized cells	605:631	The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively.					
32661565	6	46	theme	418 g ·L-1	1064:1073	arg1	acid					1085:1088	418 g ·L-1 nicotinic acid	1064:1088	418 g ·L-1 nicotinic acid	1064:1088	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	1	47	theme	acid	150:153	arg1	yield					160:164	nicotinic acid (NA) yield	140:164	nicotinic acid (NA) yield	140:164	To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance.					
32661565	6	48	theme	feeding	1017:1023	arg1	concentration					1025:1037	feeding concentration	1017:1037	feeding concentration of 250 mM	1017:1047	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	2	49	theme	%	516:516	arg1	silica					518:523	2.0% silica	513:523	2.0% silica	513:523	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	2	49	theme	%	516:516	arg1	concentrations					454:467	The concentrations	450:467	The concentrations of inorganic materials	450:490	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32661565	6	50	theme	immobilized	1108:1118	arg1	cells					1120:1124	the SA-PVA immobilized cells	1097:1124	the SA-PVA immobilized cells	1097:1124	The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.					
32661565	2	51	theme	%	532:532	arg1	CaCO3					534:538	0.6% CaCO3	529:538	0.6% CaCO3	529:538	The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3.					
32795573	7	0	theme	granule	1120:1126	arg1	volume					1128:1133	the granule volume	1116:1133	the granule volume	1116:1133	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	6	1	theme	Modified	699:706	arg1	starches					708:715	Modified starches	699:715	Modified starches	699:715	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	3	2	theme	reaction	499:506	arg1	mixture					508:514	reaction mixture	499:514	reaction mixture	499:514	The influence of the composition of reaction mixture on RE was investigated.					
32795573	5	3	theme	CaO	635:637	arg1	starches					648:655	CaO cationic starches	635:655	CaO cationic starches with maximum DS of 0.28	635:679	Meanwhile, without addition of CaO cationic starches with maximum DS of 0.28 were synthesized.					
32795573	8	4	with	CS	1150:1151	arg1	solubility					1181:1190	cold water solubility	1170:1190	cold water solubility	1170:1190	Consequently, CS with high DS and cold water solubility were obtained.					
32795573	8	4	with	CS	1150:1151	arg1	DS					1163:1164	high DS	1158:1164	high DS	1158:1164	Consequently, CS with high DS and cold water solubility were obtained.					
32795573	3	5	theme	mixture	508:514	arg1	composition					484:494	the composition	480:494	the composition of reaction mixture	480:514	The influence of the composition of reaction mixture on RE was investigated.					
32795573	6	6	dep	Fourier	748:754	arg1	means					739:743	means	739:743	means	739:743	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	6	6	dep	Fourier	748:754	arg1	transform					756:764	transform	756:764	transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies	756:923	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	4	7	theme	0.84	562:565	arg1	DS					556:557	highest DS	548:557	highest DS of 0.84	548:565	CS with highest DS of 0.84 were obtained by using CaO additive.					
32795573	8	8	theme	high	1158:1161	arg1	DS					1163:1164	high DS	1158:1164	high DS	1158:1164	Consequently, CS with high DS and cold water solubility were obtained.					
32795573	5	9	theme	cationic	639:646	arg1	starches					648:655	CaO cationic starches	635:655	CaO cationic starches with maximum DS of 0.28	635:679	Meanwhile, without addition of CaO cationic starches with maximum DS of 0.28 were synthesized.					
32795573	7	10	theme	reaction	972:979	arg1	mixture					981:987	reaction mixture	972:987	reaction mixture	972:987	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	2	11	dep	0.84	379:382	arg1	to					376:377	to	376:377	to	376:377	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	4	12	with	CS	540:541	arg1	DS					556:557	highest DS	548:557	highest DS of 0.84	548:565	CS with highest DS of 0.84 were obtained by using CaO additive.					
32795573	6	13	theme	solubility	906:915	arg1	studies					917:923	solubility studies	906:923	solubility studies	906:923	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	5	14	theme	starches	648:655	arg1	addition					623:630	addition	623:630	addition of CaO cationic starches with maximum DS of 0.28	623:679	Meanwhile, without addition of CaO cationic starches with maximum DS of 0.28 were synthesized.					
32795573	6	15	theme	diffraction	792:802	arg1	spectroscopy					804:815	X-ray powder diffraction spectroscopy	779:815	X-ray powder diffraction spectroscopy	779:815	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	6	16	theme	powder	785:790	arg1	diffraction					792:802	X-ray powder diffraction	779:802	X-ray powder diffraction spectroscopy	779:815	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	6	17	dep	transform	756:764	arg1	infrared					766:773	infrared	766:773	transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies	756:923	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	1	18	theme	potato	200:205	arg1	starch					207:212	potato starch	200:212	potato starch	200:212	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	6	19	theme	X-ray	779:783	arg1	diffraction					792:802	X-ray powder diffraction	779:802	X-ray powder diffraction spectroscopy	779:815	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	2	20	theme	semidry	440:446	arg1	cationization					448:460	semidry cationization	440:460	semidry cationization	440:460	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	1	21	theme	Cationic	119:126	arg1	starches					128:135	Cationic starches	119:135	Cationic starches (CS) with preserved microgranular structure	119:179	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	1	21	theme	Cationic	119:126	arg1	CS					138:139	CS	138:139	CS	138:139	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	7	22	theme	granules	1046:1053	arg1	layers					1032:1037	the deeper layers	1021:1037	the deeper layers of the granules	1021:1053	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	2	23	theme	%	397:397	arg1	%					406:406	47.1% to 99.6%	393:406	47.1% to 99.6%	393:406	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	8	24	theme	cold	1170:1173	arg1	solubility					1181:1190	cold water solubility	1170:1190	cold water solubility	1170:1190	Consequently, CS with high DS and cold water solubility were obtained.					
32795573	4	25	theme	highest	548:554	arg1	DS					556:557	highest DS	548:557	highest DS of 0.84	548:565	CS with highest DS of 0.84 were obtained by using CaO additive.					
32795573	5	26	theme	0.28	676:679	arg1	DS					670:671	maximum DS	662:671	maximum DS of 0.28	662:679	Meanwhile, without addition of CaO cationic starches with maximum DS of 0.28 were synthesized.					
32795573	7	27	theme	CaO	963:965	arg1	addition					951:958	addition	951:958	addition of CaO into reaction mixture	951:987	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	2	28	theme	efficiency	342:351	arg1	degree					301:306	degree	301:306	degree of substitution (DS) and reaction efficiency (RE)	301:356	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	1	29	theme	3-chloro-2-hydroxypropyltrimethyl	220:252	arg1	chloride					263:270	3-chloro-2-hydroxypropyltrimethyl ammonium chloride	220:270	3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent	220:290	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	1	30	with	starches	128:135	arg1	structure					171:179	preserved microgranular structure	147:179	preserved microgranular structure	147:179	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	2	31	with	CS	293:294	arg1	degree					301:306	degree	301:306	degree of substitution (DS) and reaction efficiency (RE)	301:356	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	6	32	theme	Brabender	874:882	arg1	viscoamilography					884:899	Brabender viscoamilography	874:899	Brabender viscoamilography	874:899	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	2	33	theme	reaction	333:340	arg1	efficiency					342:351	reaction efficiency	333:351	reaction efficiency (RE)	333:356	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	2	33	theme	reaction	333:340	arg1	RE					354:355	RE	354:355	RE	354:355	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	1	34	theme	ammonium	254:261	arg1	chloride					263:270	3-chloro-2-hydroxypropyltrimethyl ammonium chloride	220:270	3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent	220:290	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	0	35	theme	starches	37:44	arg1	properties					14:23	properties	14:23	properties	14:23	Structure and properties of cationic starches synthesized by using 3-chloro-2-hydroxypropyltrimethylammonium chloride.					
32795573	0	35	theme	starches	37:44	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and properties of cationic starches synthesized by using 3-chloro-2-hydroxypropyltrimethylammonium chloride.					
32795573	0	36	theme	cationic	28:35	arg1	starches					37:44	cationic starches	28:44	cationic starches	28:44	Structure and properties of cationic starches synthesized by using 3-chloro-2-hydroxypropyltrimethylammonium chloride.					
32795573	7	37	theme	attached	1059:1066	arg1	groups					1077:1082	attached cationic groups	1059:1082	attached cationic groups	1059:1082	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	2	38	dep	%	406:406	arg1	to					399:400	to	399:400	to	399:400	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	8	39	theme	water	1175:1179	arg1	solubility					1181:1190	cold water solubility	1170:1190	cold water solubility	1170:1190	Consequently, CS with high DS and cold water solubility were obtained.					
32795573	7	40	theme	cationic	1068:1075	arg1	groups					1077:1082	attached cationic groups	1059:1082	attached cationic groups	1059:1082	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	6	41	theme	histological	848:859	arg1	examination					861:871	histological examination	848:871	histological examination	848:871	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	7	42	theme	deeper	1025:1030	arg1	layers					1032:1037	the deeper layers	1021:1037	the deeper layers of the granules	1021:1053	It was confirmed that by addition of CaO into reaction mixture, the cationization proceeded in the deeper layers of the granules and attached cationic groups were more evenly distributed in the granule volume.					
32795573	3	43	theme	composition	484:494	arg1	influence					467:475	The influence	463:475	The influence of the composition of reaction mixture on RE	463:520	The influence of the composition of reaction mixture on RE was investigated.					
32795573	5	44	theme	maximum	662:668	arg1	DS					670:671	maximum DS	662:671	maximum DS of 0.28	662:679	Meanwhile, without addition of CaO cationic starches with maximum DS of 0.28 were synthesized.					
32795573	4	45	dep	additive	594:601	arg1	CaO					590:592	CaO	590:592	CaO	590:592	CS with highest DS of 0.84 were obtained by using CaO additive.					
32795573	2	46	theme	substitution	311:322	arg1	degree					301:306	degree	301:306	degree of substitution (DS) and reaction efficiency (RE)	301:356	CS with degree of substitution (DS) and reaction efficiency (RE) ranging from 0.05 to 0.84 and from 47.1% to 99.6%, respectively, were obtained by semidry cationization.					
32795573	3	47	from	influence	467:475	arg1	RE					519:520	RE	519:520	RE	519:520	The influence of the composition of reaction mixture on RE was investigated.					
32795573	1	48	theme	preserved	147:155	arg1	structure					171:179	preserved microgranular structure	147:179	preserved microgranular structure	147:179	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	1	49	theme	cationic	275:282	arg1	reagent					284:290	cationic reagent	275:290	cationic reagent	275:290	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	6	50	theme	electron	827:834	arg1	microscopy					836:845	scanning electron microscopy	818:845	scanning electron microscopy	818:845	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	1	51	theme	microgranular	157:169	arg1	structure					171:179	preserved microgranular structure	147:179	preserved microgranular structure	147:179	Cationic starches (CS) with preserved microgranular structure were prepared from potato starch, with 3-chloro-2-hydroxypropyltrimethyl ammonium chloride as cationic reagent.					
32795573	0	52	theme	3-chloro-2-hydroxypropyltrimethylammonium	67:107	arg1	chloride					109:116	3-chloro-2-hydroxypropyltrimethylammonium chloride	67:116	3-chloro-2-hydroxypropyltrimethylammonium chloride	67:116	Structure and properties of cationic starches synthesized by using 3-chloro-2-hydroxypropyltrimethylammonium chloride.					
32795573	6	53	theme	scanning	818:825	arg1	microscopy					836:845	scanning electron microscopy	818:845	scanning electron microscopy	818:845	Modified starches were characterized by means of Fourier transform infrared and X-ray powder diffraction spectroscopy, scanning electron microscopy, histological examination, Brabender viscoamilography, and solubility studies.					
32795573	5	54	with	starches	648:655	arg1	DS					670:671	maximum DS	662:671	maximum DS of 0.28	662:679	Meanwhile, without addition of CaO cationic starches with maximum DS of 0.28 were synthesized.					
34884718	8	0	theme	virus	1403:1407	arg1	protein					1365:1371	GP160-the envelope protein	1346:1371	GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1346:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	4	1	theme	β-blocks	695:702	arg1	distribution					670:681	the blockwise distribution	656:681	the blockwise distribution of the long β-blocks along the polysaccharide chain	656:733	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	7	2	theme	envelope	1156:1163	arg1	proteins					1165:1172	the envelope proteins	1152:1172	the envelope proteins of three different viruses	1152:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	1	3	theme	hybrid	136:141	arg1	κ/β-CRG					160:166	κ/β-CRG	160:166	κ/β-CRG	160:166	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	1	3	theme	hybrid	136:141	arg1	κ/β-carrageenan					143:157	the hybrid κ/β-carrageenan	132:157	the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus	132:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	9	4	theme	antiviral	1512:1520	arg1	activity					1522:1529	significantly higher antiviral activity	1491:1529	significantly higher antiviral activity	1491:1529	The CRG oligosaccharides displayed significantly higher antiviral activity.					
34884718	0	5	theme	Potential	67:75	arg1	Inhibitor					77:85	A Novel Potential Inhibitor	59:85	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.	0:95	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.					
34884718	1	6	theme	κ/β-carrageenan	143:157	arg1	structure					119:127	the structure	115:127	the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus	115:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	8	7	theme	transduction	1281:1292	arg1	efficiency					1294:1303	the transduction efficiency	1277:1303	the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1277:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	0	8	theme	HIV-1	90:94	arg1	Inhibitor					77:85	A Novel Potential Inhibitor	59:85	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.	0:95	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.					
34884718	4	9	theme	ι-blocks	783:790	arg1	inclusion					745:753	the inclusion	741:753	the inclusion of κ/β, μ/ν-blocks and some ι-blocks	741:790	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	3	10	dep	composition	395:405	arg1	The					391:393	The	391:393	The	391:393	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	2	11	theme	chemical	281:288	arg1	depolymerization					304:319	the chemical and enzymatic depolymerization	277:319	the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction	277:388	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	3	12	theme	MALDI-TOFMS	511:521	arg1	method					533:538	the negative ion tandem MALDI-TOFMS and ESIMS method	487:538	the negative ion tandem MALDI-TOFMS and ESIMS method	487:538	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	2	13	with	depolymerization	304:319	arg1	κ-carrageenase					337:350	κ-carrageenase	337:350	κ-carrageenase	337:350	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	2	13	with	depolymerization	304:319	arg1	fraction					381:388	the enzyme-resistant fraction	360:388	its the enzyme-resistant fraction	356:388	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	7	14	theme	particles	1125:1133	arg1	efficiency					1078:1087	the transduction efficiency	1061:1087	the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses	1061:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	8	15	theme	envelope	1356:1363	arg1	protein					1365:1371	GP160-the envelope protein	1346:1371	GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1346:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	8	16	theme	Jurkat	1442:1447	arg1	cells					1449:1453	T-lymphocyte Jurkat cells	1429:1453	T-lymphocyte Jurkat cells	1429:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	8	17	theme	lentiviral	1308:1317	arg1	particles					1319:1327	lentiviral particles	1308:1327	lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1308:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	3	18	theme	κ/β-	451:454	arg1	CRG					456:458	κ/β- CRG	451:458	κ/β- CRG	451:458	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	8	19	theme	immunodeficiency	1386:1401	arg1	virus					1403:1407	the human immunodeficiency virus HIV-1-when	1376:1418	the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1376:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	5	20	theme	desulfated	810:819	arg1	κ/β-CRG					821:827	the desulfated κ/β-CRG	806:827	the desulfated κ/β-CRG	806:827	Furthermore, the desulfated κ/β-CRG was shown to contain of -G-D- repeating units up to 3.5 kDa.					
34884718	3	21	theme	CRG	456:458	arg1	units					442:446	the repetition units	427:446	the repetition units of κ/β- CRG	427:458	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	7	22	theme	replication-defective	1092:1112	arg1	particles					1125:1133	replication-defective lentiviral particles	1092:1133	replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses	1092:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	4	23	theme	blockwise	660:668	arg1	distribution					670:681	the blockwise distribution	656:681	the blockwise distribution of the long β-blocks along the polysaccharide chain	656:733	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	5	24	dep	kDa	885:887	arg1	up					875:876	up	875:876	up	875:876	Furthermore, the desulfated κ/β-CRG was shown to contain of -G-D- repeating units up to 3.5 kDa.					
34884718	3	25	theme	units	442:446	arg1	composition					395:405	composition	395:405	composition	395:405	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	3	25	theme	units	442:446	arg1	distribution					411:422	distribution	411:422	distribution	411:422	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	0	26	theme	New	0:2	arg1	Insights					4:11	New Insights	0:11	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.	0:95	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.					
34884718	6	27	theme	viruses	971:977	arg1	replication					948:958	the replication	944:958	the replication of several viruses	944:977	Previous studies have demonstrated that CRGs suppress the replication of several viruses.					
34884718	7	28	theme	lentiviral	1114:1123	arg1	particles					1125:1133	replication-defective lentiviral particles	1092:1133	replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses	1092:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	5	29	theme	-G-D-	853:857	arg1	units					869:873	-G-D- repeating units	853:873	-G-D- repeating units up to 3.5 kDa	853:887	Furthermore, the desulfated κ/β-CRG was shown to contain of -G-D- repeating units up to 3.5 kDa.					
34884718	5	30	theme	repeating	859:867	arg1	units					869:873	-G-D- repeating units	853:873	-G-D- repeating units up to 3.5 kDa	853:887	Furthermore, the desulfated κ/β-CRG was shown to contain of -G-D- repeating units up to 3.5 kDa.					
34884718	4	31	theme	μ/ν-blocks	763:772	arg1	inclusion					745:753	the inclusion	741:753	the inclusion of κ/β, μ/ν-blocks and some ι-blocks	741:790	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	2	32	theme	Carrageenan	226:236	arg1	oligosaccharides					238:253	Carrageenan oligosaccharides	226:253	Carrageenan oligosaccharides	226:253	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	2	33	theme	enzymatic	294:302	arg1	depolymerization					304:319	the chemical and enzymatic depolymerization	277:319	the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction	277:388	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	1	34	theme	New	97:99	arg1	insights					101:108	New insights	97:108	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus	97:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	7	35	theme	viruses	1193:1199	arg1	proteins					1165:1172	the envelope proteins	1152:1172	the envelope proteins of three different viruses	1152:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	1	36	theme	red	176:178	arg1	alga					180:183	the red alga	172:183	the red alga Tichocarpus crinitus	172:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	7	37	theme	different	1183:1191	arg1	viruses					1193:1199	three different viruses	1177:1199	three different viruses	1177:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	4	38	theme	polysaccharide	714:727	arg1	chain					729:733	the polysaccharide chain	710:733	the polysaccharide chain	710:733	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	8	39	theme	GP160-the	1346:1354	arg1	protein					1365:1371	GP160-the envelope protein	1346:1371	GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1346:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	3	40	theme	hybrid	594:599	arg1	structure					601:609	the hybrid structure	590:609	the hybrid structure of this polysaccharide	590:632	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	1	41	theme	alga	180:183	arg1	κ/β-CRG					160:166	κ/β-CRG	160:166	κ/β-CRG	160:166	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	1	41	theme	alga	180:183	arg1	κ/β-carrageenan					143:157	the hybrid κ/β-carrageenan	132:157	the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus	132:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	3	42	theme	repetition	431:440	arg1	units					442:446	the repetition units	427:446	the repetition units of κ/β- CRG	427:458	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	7	43	theme	transduction	1065:1076	arg1	efficiency					1078:1087	the transduction efficiency	1061:1087	the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses	1061:1199	Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses.					
34884718	8	44	theme	human	1380:1384	arg1	virus					1403:1407	the human immunodeficiency virus HIV-1-when	1376:1418	the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1376:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	1	45	theme	Tichocarpus	185:195	arg1	alga					180:183	the red alga	172:183	the red alga Tichocarpus crinitus	172:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	8	46	theme	particles	1319:1327	arg1	efficiency					1294:1303	the transduction efficiency	1277:1303	the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells	1277:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	6	47	theme	several	963:969	arg1	viruses					971:977	several viruses	963:977	several viruses	963:977	Previous studies have demonstrated that CRGs suppress the replication of several viruses.					
34884718	3	48	theme	ion	500:502	arg1	MALDI-TOFMS					511:521	negative ion tandem MALDI-TOFMS	491:521	negative ion tandem MALDI-TOFMS	491:521	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	0	49	theme	Kappa/Beta-Carrageenan	35:56	arg1	Structure					22:30	the Structure	18:30	the Structure of Kappa/Beta-Carrageenan	18:56	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.					
34884718	3	50	theme	tandem	504:509	arg1	MALDI-TOFMS					511:521	negative ion tandem MALDI-TOFMS	491:521	negative ion tandem MALDI-TOFMS	491:521	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	9	51	theme	CRG	1460:1462	arg1	oligosaccharides					1464:1479	The CRG oligosaccharides	1456:1479	The CRG oligosaccharides	1456:1479	The CRG oligosaccharides displayed significantly higher antiviral activity.					
34884718	3	52	theme	polysaccharide	619:632	arg1	structure					601:609	the hybrid structure	590:609	the hybrid structure of this polysaccharide	590:632	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	4	53	theme	long	690:693	arg1	β-blocks					695:702	the long β-blocks	686:702	the long β-blocks	686:702	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	4	54	theme	κ/β	758:760	arg1	inclusion					745:753	the inclusion	741:753	the inclusion of κ/β, μ/ν-blocks and some ι-blocks	741:790	An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks.					
34884718	8	55	theme	T-lymphocyte	1429:1440	arg1	cells					1449:1453	T-lymphocyte Jurkat cells	1429:1453	T-lymphocyte Jurkat cells	1429:1453	We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells.					
34884718	1	56	dep	Tichocarpus	185:195	arg1	crinitus					197:204	crinitus	197:204	crinitus	197:204	New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained.					
34884718	2	57	theme	κ/β-CRG	324:330	arg1	depolymerization					304:319	the chemical and enzymatic depolymerization	277:319	the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction	277:388	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	0	58	theme	Novel	61:65	arg1	Inhibitor					77:85	A Novel Potential Inhibitor	59:85	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.	0:95	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.					
34884718	0	59	dep	Insights	4:11	arg1	Inhibitor					77:85	A Novel Potential Inhibitor	59:85	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.	0:95	New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.					
34884718	3	60	theme	ESIMS	527:531	arg1	method					533:538	the negative ion tandem MALDI-TOFMS and ESIMS method	487:538	the negative ion tandem MALDI-TOFMS and ESIMS method	487:538	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	3	61	theme	negative	491:498	arg1	MALDI-TOFMS					511:521	negative ion tandem MALDI-TOFMS	491:521	negative ion tandem MALDI-TOFMS	491:521	The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide.					
34884718	6	62	theme	Previous	890:897	arg1	studies					899:905	Previous studies	890:905	Previous studies	890:905	Previous studies have demonstrated that CRGs suppress the replication of several viruses.					
34884718	2	63	theme	enzyme-resistant	364:379	arg1	fraction					381:388	the enzyme-resistant fraction	360:388	its the enzyme-resistant fraction	356:388	Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction.					
34884718	9	64	theme	higher	1505:1510	arg1	activity					1522:1529	significantly higher antiviral activity	1491:1529	significantly higher antiviral activity	1491:1529	The CRG oligosaccharides displayed significantly higher antiviral activity.					
33277271	4	0	theme	mucin	1047:1051	arg1	addition					1035:1042	addition	1035:1042	addition of mucin	1035:1051	As such, the effect of A.mucinihpila administration was investigated with and without addition of mucin.					
33277271	8	1	theme	endpoints	1635:1643	arg1	assessment					1596:1605	the assessment	1592:1605	the assessment of physiologically relevant endpoints	1592:1643	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	9	2	theme	different	1908:1916	arg1	donors					1918:1923	four different donors	1903:1923	four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1903:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	5	3	dep	4	1290:1290	arg1	-					1288:1288	-	1288:1288	-	1288:1288	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	12	4	theme	higher	2514:2519	arg1	acetate					2521:2527	significantly higher acetate	2500:2527	significantly higher acetate	2500:2527	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	3	5	theme	A.	685:686	arg1	behavior					700:707	A. muciniphila behavior	685:707	A. muciniphila behavior	685:707	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	8	6	theme	mechanistic	1726:1736	arg1	characterization					1738:1753	the in-depth mechanistic characterization	1713:1753	the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome	1713:1807	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	1	7	theme	IMPORTANCE	162:171	arg1	Research					181:188	IMPORTANCE SECTION Research	162:188	IMPORTANCE SECTION Research	162:188	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	12	8	theme	muciniphila	2760:2770	arg1	supplementation					2738:2752	the supplementation	2734:2752	the supplementation of A. muciniphila together with mucin	2734:2790	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	9	9	theme	validated	1930:1938	arg1	model					1961:1965	a validated, controlled in vitro model	1928:1965	a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1928:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	9	10	from	effect	1835:1840	arg1	community					1888:1896	the endogenous community	1873:1896	the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1873:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	11	11	theme	A.	2303:2304	arg1	supplementation					2318:2332	A. muciniphila supplementation	2303:2332	A. muciniphila supplementation	2303:2332	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	8	12	theme	effects	1770:1776	arg1	characterization					1738:1753	the in-depth mechanistic characterization	1713:1753	the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome	1713:1807	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	10	13	theme	colon	2121:2125	arg1	environment					2127:2137	the colon environment	2117:2137	the colon environment	2117:2137	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	6	14	theme	biotherapeutic	1418:1431	arg1	agent					1433:1437	a biotherapeutic agent	1416:1437	a biotherapeutic agent	1416:1437	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	6	14	theme	biotherapeutic	1418:1431	arg1	It					1342:1343	It	1342:1343	It	1342:1343	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	1	15	theme	biomarkers	213:222	arg1	identification					195:208	identification	195:208	identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health	195:316	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	3	16	theme	probiotic	731:739	arg1	administration					741:754	its probiotic administration	727:754	its probiotic administration	727:754	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	9	17	theme	gut	1974:1976	arg1	ecosystem					1988:1996	the gut microbial ecosystem	1970:1996	the gut microbial ecosystem (SHIME)	1970:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	9	17	theme	gut	1974:1976	arg1	SHIME					1999:2003	SHIME	1999:2003	SHIME	1999:2003	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	0	18	theme	m	148:148	arg1	uciniphila					150:159	Akkermansia m uciniphila	136:159	Akkermansia m uciniphila	136:159	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	8	19	theme	microbiome	1798:1807	arg1	level					1785:1789	the level	1781:1789	the level of the microbiome	1781:1807	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	9	20	from	donors	1918:1923	arg1	model					1961:1965	a validated, controlled in vitro model	1928:1965	a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1928:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	9	20	from	donors	1918:1923	arg1	community					1888:1896	the endogenous community	1873:1896	the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1873:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	9	21	theme	ecosystem	1988:1996	arg1	model					1961:1965	a validated, controlled in vitro model	1928:1965	a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1928:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	3	22	theme	microbial	763:771	arg1	study					788:792	the microbial ecosystem.This study	759:792	the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	759:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	0	23	theme	Important	38:46	arg1	Mucin					0:4	Mucin	0:4	Mucin as a Functional Niche	0:26	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	0	23	theme	Important	38:46	arg1	Driver					48:53	a More Important Driver	31:53	a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila	31:159	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	3	24	theme	complex	595:601	arg1	ecosystem					613:621	the complex microbial ecosystem	591:621	the complex microbial ecosystem in the colon	591:634	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	3	25	theme	endogenous	868:877	arg1	community					879:887	the endogenous community	864:887	the endogenous community while also taking into account its nutritional specificity	864:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	4	26	theme	A.mucinihpila	972:984	arg1	administration					986:999	A.mucinihpila administration	972:999	A.mucinihpila administration	972:999	As such, the effect of A.mucinihpila administration was investigated with and without addition of mucin.					
33277271	1	27	theme	Akkermansia	327:337	arg1	muciniphila					339:349	Akkermansia muciniphila	327:349	Akkermansia muciniphila	327:349	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	1	28	dep	composition	275:285	arg1	improve					303:309	improve	303:309	to improve health	300:316	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	1	28	dep	composition	275:285	arg1	the					260:262	the	260:262	the	260:262	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	12	29	theme	several	2606:2612	arg1	Lachnospiraceae					2693:2707	Lachnospiraceae	2693:2707	Lachnospiraceae	2693:2707	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	29	theme	several	2606:2612	arg1	species					2614:2620	several species	2606:2620	several species	2606:2620	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	29	theme	several	2606:2612	arg1	Ruminococcus					2649:2660	Ruminococcus	2649:2660	Ruminococcus	2649:2660	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	29	theme	several	2606:2612	arg1	muciniphila					2636:2646	muciniphila	2636:2646	muciniphila	2636:2646	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	29	theme	several	2606:2612	arg1	XIVa					2683:2686	Clostridium cluster XIVa	2663:2686	Clostridium cluster XIVa	2663:2686	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	9	30	dep	in	1952:1953	arg1	vitro					1955:1959	vitro	1955:1959	vitro	1955:1959	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	7	31	theme	human	1547:1551	arg1	trials					1566:1571	human intervention trials	1547:1571	human intervention trials	1547:1571	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	10	32	theme	endogenous	2192:2201	arg1	community					2203:2211	the endogenous community	2188:2211	the endogenous community	2188:2211	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	0	33	theme	Microbiota	71:80	arg1	Composition					82:92	in Vitro Gut Microbiota Composition	58:92	in Vitro Gut Microbiota Composition	58:92	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	5	34	theme	mucin	1101:1105	arg1	presence					1107:1114	mucin presence	1101:1114	mucin presence	1101:1114	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	12	35	theme	mucin	2838:2842	arg1	effect					2828:2833	the observed prebiotic-like effect	2800:2833	the observed prebiotic-like effect of mucin in inducing compositional changes	2800:2876	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	3	36	from	ecosystem	613:621	arg1	colon					630:634	the colon	626:634	the colon	626:634	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	1	37	theme	microbiota	264:273	arg1	composition					275:285	microbiota composition	264:285	microbiota composition	264:285	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	3	38	theme	nutritional	924:934	arg1	specificity					936:946	its nutritional specificity	920:946	its nutritional specificity	920:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	2	39	theme	interesting	419:429	arg1	degrader					380:387	a mucin degrader	372:387	a mucin degrader	372:387	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	2	39	theme	interesting	419:429	arg1	niche.					479:484	the interesting but not-fully described host-glycan degradation niche.	415:484	the interesting but not-fully described host-glycan degradation niche.	415:484	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	10	40	from	action	2097:2102	arg1	environment					2127:2137	the colon environment	2117:2137	the colon environment	2117:2137	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	10	40	from	action	2097:2102	arg1	interplay					2144:2152	the interplay	2140:2152	the interplay between mucin	2140:2166	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	10	40	from	action	2097:2102	arg1	community					2203:2211	the endogenous community	2188:2211	the endogenous community	2188:2211	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	10	40	from	action	2097:2102	arg1	muciniphila					2172:2182	muciniphila	2172:2182	muciniphila	2172:2182	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	7	41	theme	mouse	1530:1534	arg1	models					1536:1541	in vivo mouse models	1522:1541	in vivo mouse models	1522:1541	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	12	42	theme	observed	2804:2811	arg1	effect					2828:2833	the observed prebiotic-like effect	2800:2833	the observed prebiotic-like effect of mucin in inducing compositional changes	2800:2876	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	3	43	dep	A.	685:686	arg1	muciniphila					688:698	muciniphila	688:698	muciniphila	688:698	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	2	44	theme	degradation	467:477	arg1	degrader					380:387	a mucin degrader	372:387	a mucin degrader	372:387	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	2	44	theme	degradation	467:477	arg1	niche.					479:484	the interesting but not-fully described host-glycan degradation niche.	415:484	the interesting but not-fully described host-glycan degradation niche.	415:484	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	7	45	theme	in	1522:1523	arg1	models					1536:1541	in vivo mouse models	1522:1541	in vivo mouse models	1522:1541	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	2	46	theme	host-glycan	455:465	arg1	degrader					380:387	a mucin degrader	372:387	a mucin degrader	372:387	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	2	46	theme	host-glycan	455:465	arg1	niche.					479:484	the interesting but not-fully described host-glycan degradation niche.	415:484	the interesting but not-fully described host-glycan degradation niche.	415:484	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	5	47	theme	probiotic	1150:1158	arg1	supplementation					1160:1174	the probiotic supplementation	1146:1174	the probiotic supplementation	1146:1174	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	9	48	theme	treatment	1860:1868	arg1	effect					1835:1840	the effect	1831:1840	the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1831:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	11	49	dep	composition	2270:2280	arg1	the					2246:2248	the	2246:2248	the	2246:2248	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	7	50	dep	in	1522:1523	arg1	vivo					1525:1528	vivo	1525:1528	vivo	1525:1528	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	12	51	dep	muciniphila	2636:2646	arg1	revealed					2720:2727	revealed	2720:2727	revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes	2720:2876	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	8	52	theme	fecal	1662:1666	arg1	samples					1668:1674	fecal samples	1662:1674	fecal samples	1662:1674	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	5	53	theme	mucin	1255:1259	arg1	%					1291:1291	1 - 4%	1286:1291	1 - 4%	1286:1291	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	5	53	theme	mucin	1255:1259	arg1	bacterium					1275:1283	an abundantly present commensal mucin degrading gut bacterium	1223:1283	an abundantly present commensal mucin degrading gut bacterium (1 - 4%)	1223:1292	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	9	54	theme	A.	1845:1846	arg1	treatment					1860:1868	A. muciniphila treatment	1845:1868	A. muciniphila treatment	1845:1868	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	7	55	theme	ongoing	1486:1492	arg1	research					1494:1501	Preliminary and ongoing research	1470:1501	Preliminary and ongoing research	1470:1501	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	12	56	theme	butyrate	2545:2552	arg1	production					2554:2563	butyrate production	2545:2563	butyrate production	2545:2563	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	6	57	theme	health	1370:1375	arg1	biomarker					1377:1385	a health biomarker	1368:1385	a health biomarker	1368:1385	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	6	57	theme	health	1370:1375	arg1	It					1342:1343	It	1342:1343	It	1342:1343	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	11	58	dep	A.	2303:2304	arg1	muciniphila					2306:2316	muciniphila	2306:2316	muciniphila	2306:2316	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	3	59	theme	research	499:506	arg1	Plenty					489:494	Plenty	489:494	Plenty of research concerning A. muciniphila	489:532	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	6	60	theme	probiotic	1459:1467	arg1	generation					1448:1457	next generation probiotic	1443:1467	next generation probiotic	1443:1467	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	6	60	theme	probiotic	1459:1467	arg1	It					1342:1343	It	1342:1343	It	1342:1343	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	7	61	theme	Preliminary	1470:1480	arg1	research					1494:1501	Preliminary and ongoing research	1470:1501	Preliminary and ongoing research	1470:1501	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	8	62	theme	relevant	1626:1633	arg1	endpoints					1635:1643	physiologically relevant endpoints	1610:1643	physiologically relevant endpoints	1610:1643	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	3	63	theme	mucins	665:670	arg1	role					657:660	the potential role	643:660	the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	643:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	5	64	with	supplementation	1160:1174	arg1	muciniphila					1208:1218	muciniphila	1208:1218	muciniphila	1208:1218	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	6	65	theme	next	1443:1446	arg1	generation					1448:1457	next generation probiotic	1443:1467	next generation probiotic	1443:1467	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	6	65	theme	next	1443:1446	arg1	It					1342:1343	It	1342:1343	It	1342:1343	It has been positioned as a health biomarker and is currently explored as a biotherapeutic agent and next generation probiotic.					
33277271	11	66	theme	community	2260:2268	arg1	composition					2270:2280	microbial community composition	2250:2280	microbial community composition	2250:2280	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	11	67	theme	microbial	2250:2258	arg1	composition					2270:2280	microbial community composition	2250:2280	microbial community composition	2250:2280	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	9	68	dep	A.	1845:1846	arg1	muciniphila					1848:1858	muciniphila	1848:1858	muciniphila	1848:1858	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	12	69	theme	prebiotic-like	2813:2826	arg1	effect					2828:2833	the observed prebiotic-like effect	2800:2833	the observed prebiotic-like effect of mucin in inducing compositional changes	2800:2876	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	10	70	theme	mucins	2107:2112	arg1	action					2097:2102	the prebiotic-like action	2078:2102	the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community	2078:2211	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	8	71	from	level	1785:1789	arg1	characterization					1738:1753	the in-depth mechanistic characterization	1713:1753	the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome	1713:1807	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	8	72	theme	in-depth	1717:1724	arg1	characterization					1738:1753	the in-depth mechanistic characterization	1713:1753	the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome	1713:1807	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	1	73	theme	SECTION	173:179	arg1	Research					181:188	IMPORTANCE SECTION Research	162:188	IMPORTANCE SECTION Research	162:188	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	3	74	from	behavior	700:707	arg1	study					788:792	the microbial ecosystem.This study	759:792	the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	759:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	0	75	theme	Functionality	98:110	arg1	Mucin					0:4	Mucin	0:4	Mucin as a Functional Niche	0:26	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	0	75	theme	Functionality	98:110	arg1	Driver					48:53	a More Important Driver	31:53	a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila	31:159	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	1	76	dep	health	232:237	arg1	modulate					251:258	modulate	251:258	to modulate the microbiota composition and activity to improve health	248:316	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	9	77	theme	in	1952:1953	arg1	model					1961:1965	a validated, controlled in vitro model	1928:1965	a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1928:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	8	78	theme	Akkermansia	1758:1768	arg1	effects					1770:1776	Akkermansia effects	1758:1776	Akkermansia effects	1758:1776	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	5	79	dep	present	1237:1243	arg1	commensal					1245:1253	commensal	1245:1253	commensal	1245:1253	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	11	80	theme	gut	2449:2451	arg1	microbiota					2453:2462	the gut microbiota	2445:2462	the gut microbiota	2445:2462	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	0	81	theme	Akkermansia	136:146	arg1	uciniphila					150:159	Akkermansia m uciniphila	136:159	Akkermansia m uciniphila	136:159	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	9	82	dep	validated	1930:1938	arg1	controlled					1941:1950	controlled	1941:1950	controlled	1941:1950	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	3	83	theme	administration	741:754	arg1	behavior					700:707	A. muciniphila behavior	685:707	A. muciniphila behavior	685:707	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	3	83	theme	administration	741:754	arg1	impact					717:722	the impact	713:722	the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	713:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	0	84	theme	uciniphila	150:159	arg1	Supplementation					117:131	Supplementation	117:131	Supplementation of Akkermansia m uciniphila	117:159	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	3	85	theme	microbial	603:611	arg1	ecosystem					613:621	the complex microbial ecosystem	591:621	the complex microbial ecosystem in the colon	591:634	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	3	86	theme	administration	846:859	arg1	impact					821:826	the impact	817:826	the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	817:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	5	87	dep	muciniphila	1208:1218	arg1	%					1291:1291	1 - 4%	1286:1291	1 - 4%	1286:1291	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	5	87	dep	muciniphila	1208:1218	arg1	bacterium					1275:1283	an abundantly present commensal mucin degrading gut bacterium	1223:1283	an abundantly present commensal mucin degrading gut bacterium (1 - 4%)	1223:1292	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	9	88	theme	microbial	1978:1986	arg1	ecosystem					1988:1996	the gut microbial ecosystem	1970:1996	the gut microbial ecosystem (SHIME)	1970:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	9	88	theme	microbial	1978:1986	arg1	SHIME					1999:2003	SHIME	1999:2003	SHIME	1999:2003	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	3	89	theme	A.	831:832	arg1	administration					846:859	A. muciniphila administration	831:859	A. muciniphila administration	831:859	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	5	90	theme	present	1237:1243	arg1	%					1291:1291	1 - 4%	1286:1291	1 - 4%	1286:1291	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	5	90	theme	present	1237:1243	arg1	bacterium					1275:1283	an abundantly present commensal mucin degrading gut bacterium	1223:1283	an abundantly present commensal mucin degrading gut bacterium (1 - 4%)	1223:1292	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	5	91	theme	gut	1271:1273	arg1	%					1291:1291	1 - 4%	1286:1291	1 - 4%	1286:1291	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	5	91	theme	gut	1271:1273	arg1	bacterium					1275:1283	an abundantly present commensal mucin degrading gut bacterium	1223:1283	an abundantly present commensal mucin degrading gut bacterium (1 - 4%)	1223:1292	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	3	92	theme	ecosystem.This	773:786	arg1	study					788:792	the microbial ecosystem.This study	759:792	the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	759:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	0	93	theme	in	58:59	arg1	Composition					82:92	in Vitro Gut Microbiota Composition	58:92	in Vitro Gut Microbiota Composition	58:92	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	7	94	theme	intervention	1553:1564	arg1	trials					1566:1571	human intervention trials	1547:1571	human intervention trials	1547:1571	Preliminary and ongoing research is mostly based on in vivo mouse models and human intervention trials.					
33277271	12	95	theme	species	2614:2620	arg1	production					2554:2563	butyrate production	2545:2563	butyrate production	2545:2563	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	95	theme	species	2614:2620	arg1	acetate					2521:2527	significantly higher acetate	2500:2527	significantly higher acetate	2500:2527	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	95	theme	species	2614:2620	arg1	increase					2594:2601	the increase	2590:2601	the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes	2590:2876	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	12	95	theme	species	2614:2620	arg1	propionate					2530:2539	propionate	2530:2539	propionate	2530:2539	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	10	96	theme	nutritional	2031:2041	arg1	specificity					2043:2053	the nutritional specificity	2027:2053	the nutritional specificity of A. muciniphila	2027:2071	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	0	97	theme	Gut	67:69	arg1	Composition					82:92	in Vitro Gut Microbiota Composition	58:92	in Vitro Gut Microbiota Composition	58:92	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	3	98	dep	A.	831:832	arg1	muciniphila					834:844	muciniphila	834:844	muciniphila	834:844	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	12	99	theme	compositional	2856:2868	arg1	changes					2870:2876	compositional changes	2856:2876	compositional changes	2856:2876	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	11	100	theme	mucin	2370:2374	arg1	addition					2376:2383	mucin addition	2370:2383	mucin addition	2370:2383	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	5	101	theme	healthy	1321:1327	arg1	individuals					1329:1339	healthy individuals	1321:1339	healthy individuals	1321:1339	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	0	102	theme	Composition	82:92	arg1	Mucin					0:4	Mucin	0:4	Mucin as a Functional Niche	0:26	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	0	102	theme	Composition	82:92	arg1	Driver					48:53	a More Important Driver	31:53	a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila	31:159	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	3	103	from	impact	717:722	arg1	study					788:792	the microbial ecosystem.This study	759:792	the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	759:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	11	104	theme	metabolic	2424:2432	arg1	changes					2434:2440	compositional and metabolic changes	2406:2440	compositional and metabolic changes in the gut microbiota	2406:2462	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	2	105	theme	mucin	374:378	arg1	degrader					380:387	a mucin degrader	372:387	a mucin degrader	372:387	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	2	105	theme	mucin	374:378	arg1	niche.					479:484	the interesting but not-fully described host-glycan degradation niche.	415:484	the interesting but not-fully described host-glycan degradation niche.	415:484	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	5	106	theme	presence	1107:1114	arg1	importance					1087:1096	the importance	1083:1096	the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals	1083:1339	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	0	107	dep	in	58:59	arg1	Vitro					61:65	Vitro	61:65	Vitro	61:65	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	12	108	theme	cluster	2675:2681	arg1	XIVa					2683:2686	Clostridium cluster XIVa	2663:2686	Clostridium cluster XIVa	2663:2686	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	11	109	theme	compositional	2406:2418	arg1	changes					2434:2440	compositional and metabolic changes	2406:2440	compositional and metabolic changes in the gut microbiota	2406:2462	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	0	110	theme	Functional	11:20	arg1	Niche					22:26	a Functional Niche	9:26	a Functional Niche	9:26	Mucin as a Functional Niche is a More Important Driver of in Vitro Gut Microbiota Composition and Functionality than Supplementation of Akkermansia m uciniphila.					
33277271	3	111	dep	community	879:887	arg1	taking					900:905	taking	900:905	taking into account its nutritional specificity	900:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	12	112	theme	mucin	2473:2477	arg1	addition					2479:2486	mucin addition	2473:2486	mucin addition	2473:2486	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	3	113	from	behavior	579:586	arg1	ecosystem					613:621	the complex microbial ecosystem	591:621	the complex microbial ecosystem in the colon	591:634	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	11	114	theme	supplementation	2318:2332	arg1	functionality					2286:2298	functionality	2286:2298	functionality	2286:2298	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	11	114	theme	supplementation	2318:2332	arg1	composition					2270:2280	microbial community composition	2250:2280	microbial community composition	2250:2280	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	4	115	theme	administration	986:999	arg1	such					952:955	such	952:955	such	952:955	As such, the effect of A.mucinihpila administration was investigated with and without addition of mucin.					
33277271	4	115	theme	administration	986:999	arg1	effect					962:967	the effect	958:967	the effect of A.mucinihpila administration	958:999	As such, the effect of A.mucinihpila administration was investigated with and without addition of mucin.					
33277271	3	116	from	impact	821:826	arg1	community					879:887	the endogenous community	864:887	the endogenous community while also taking into account its nutritional specificity	864:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	11	117	from	effects	2235:2241	arg1	functionality					2286:2298	functionality	2286:2298	functionality	2286:2298	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	11	117	from	effects	2235:2241	arg1	composition					2270:2280	microbial community composition	2250:2280	microbial community composition	2250:2280	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	2	118	theme	described	445:453	arg1	degrader					380:387	a mucin degrader	372:387	a mucin degrader	372:387	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	2	118	theme	described	445:453	arg1	niche.					479:484	the interesting but not-fully described host-glycan degradation niche.	415:484	the interesting but not-fully described host-glycan degradation niche.	415:484	As a mucin degrader, A. muciniphila colonizes the interesting but not-fully described host-glycan degradation niche., .					
33277271	3	119	theme	potential	647:655	arg1	role					657:660	the potential role	643:660	the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity	643:946	Plenty of research concerning A. muciniphila has been done, but little is known about its behavior in the complex microbial ecosystem in the colon, about the potential role of mucins to influence A. muciniphila behavior and the impact of its probiotic administration on the microbial ecosystem.This study aimed at investigating the impact of A. muciniphila administration on the endogenous community while also taking into account its nutritional specificity.					
33277271	5	120	theme	supplementation	1160:1174	arg1	efficiency					1132:1141	the efficiency	1128:1141	the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals	1128:1339	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	10	121	theme	muciniphila	2061:2071	arg1	specificity					2043:2053	the nutritional specificity	2027:2053	the nutritional specificity of A. muciniphila	2027:2071	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	12	122	theme	Clostridium	2663:2673	arg1	XIVa					2683:2686	Clostridium cluster XIVa	2663:2686	Clostridium cluster XIVa	2663:2686	Indeed, mucin addition resulted in significantly higher acetate, propionate and butyrate production for all four donors, and the increase of several species, including A. muciniphila, Ruminococcus, Clostridium cluster XIVa, and Lachnospiraceae This study revealed that the supplementation of A. muciniphila together with mucin limited the observed prebiotic-like effect of mucin in inducing compositional changes.					
33277271	11	123	from	changes	2434:2440	arg1	microbiota					2453:2462	the gut microbiota	2445:2462	the gut microbiota	2445:2462	The effects on the microbial community composition and functionality of A. muciniphila supplementation without mucin were limited, whereas mucin addition successfully induced compositional and metabolic changes in the gut microbiota.					
33277271	8	124	theme	samples	1668:1674	arg1	analysis					1650:1657	the analysis	1646:1657	the analysis of fecal samples	1646:1674	While these allow the assessment of physiologically relevant endpoints, the analysis of fecal samples presents limitations with respect to the in-depth mechanistic characterization of Akkermansia effects at the level of the microbiome.					
33277271	1	125	theme	gut	228:230	arg1	health					232:237	gut health	228:237	gut health	228:237	IMPORTANCE SECTION Research into identification of biomarkers for gut health and ways to modulate the microbiota composition and activity to improve health, has put Akkermansia muciniphila in the spotlight.					
33277271	9	126	theme	endogenous	1877:1886	arg1	community					1888:1896	the endogenous community	1873:1896	the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME)	1873:2004	We aimed to evaluate the effect of A. muciniphila treatment on the endogenous community from four different donors in a validated, controlled in vitro model of the gut microbial ecosystem (SHIME).					
33277271	10	127	theme	prebiotic-like	2082:2095	arg1	action					2097:2102	the prebiotic-like action	2078:2102	the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community	2078:2211	Taking into account the nutritional specificity of A. muciniphila, and the prebiotic-like action of mucins in the colon environment, the interplay between mucin, A. muciniphila and the endogenous community was investigated.					
33277271	5	128	theme	degrading	1261:1269	arg1	%					1291:1291	1 - 4%	1286:1291	1 - 4%	1286:1291	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33277271	5	128	theme	degrading	1261:1269	arg1	bacterium					1275:1283	an abundantly present commensal mucin degrading gut bacterium	1223:1283	an abundantly present commensal mucin degrading gut bacterium (1 - 4%)	1223:1292	This allowed us to elucidate the importance of mucin presence to modulate the efficiency of the probiotic supplementation with A. muciniphila Akkermansia muciniphila is an abundantly present commensal mucin degrading gut bacterium (1 - 4%) , widely distributed among healthy individuals.					
33685703	5	0	dep	P3	1141:1142	arg1	d					1151:1151	57-84 d	1145:1151	P3; 57-84 d	1141:1151	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	10	1	theme	feeding	1705:1711	arg1	behavior					1713:1720	feeding behavior	1705:1720	feeding behavior	1705:1720	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	11	2	theme	matter	1949:1954	arg1	kg					1939:1940	kg	1939:1940	kg	1939:1940	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	11	2	theme	matter	1949:1954	arg1	matter					1949:1954	dry matter	1945:1954	dry matter	1945:1954	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	4	3	dep	MR	818:819	arg1	HL					822:823	HL	822:823	HL; 17% fat; 44% lactose	822:845	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	12	4	theme	HL	2065:2066	arg1	calves					2068:2073	HL calves	2065:2073	HL calves	2065:2073	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	5	5	dep	postweaning	1128:1138	arg1	P3					1141:1142	P3	1141:1142	P3; 57-84 d	1141:1151	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	2	6	theme	voluntary	497:505	arg1	intake					512:517	voluntary feed intake	497:517	voluntary feed intake	497:517	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	4	7	theme	44	835:836	arg1	%					837:837	%	837:837	%	837:837	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	2	8	from	MR	491:492	arg1	effects					439:445	the effects	435:445	the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior	435:559	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	3	9	theme	age	611:613	arg1	kg					628:629	46.4 ± 0.77 kg	616:629	46.4 ± 0.77 kg of body weight	616:644	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	3	9	theme	age	611:613	arg1	d					606:606	2.1 ± 0.16 d	595:606	2.1 ± 0.16 d of age	595:613	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	3	9	theme	age	611:613	arg1	calves					587:592	Thirty-two male Holstein calves	562:592	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW)	562:649	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	14	10	theme	acids	2521:2525	arg1	levels					2527:2532	Plasma cholesterol and nonesterified fatty acids levels	2478:2532	Plasma cholesterol and nonesterified fatty acids levels	2478:2532	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	1	11	theme	diet	378:381	arg1	density					335:341	a lower energy density	320:341	a lower energy density	320:341	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	1	11	theme	diet	378:381	arg1	ratio					365:369	energy-to-protein ratio	347:369	energy-to-protein ratio	347:369	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	7	12	theme	age	1320:1322	arg1	d					1315:1315	14.2 ± 0.5 d	1304:1315	14.2 ± 0.5 d of age	1304:1322	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	13	13	theme	metabolizable	2382:2394	arg1	intake					2403:2408	metabolizable energy intake	2382:2408	metabolizable energy intake	2382:2408	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	9	14	theme	complete	1596:1603	arg1	withdrawal					1610:1619	complete milk withdrawal	1596:1619	complete milk withdrawal by 57 d	1596:1627	During P2, calves were gradually weaned until complete milk withdrawal by 57 d and then monitored until 84 d (P3).					
33685703	1	15	theme	whole	146:150	arg1	milk					152:155	Holstein whole milk	137:155	Holstein whole milk	137:155	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	2	16	theme	growth	520:525	arg1	performance					527:537	growth performance	520:537	growth performance	520:537	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	4	17	theme	23	871:872	arg1	%					873:873	%	873:873	%	873:873	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	3	18	theme	0.77	623:626	arg1	±					621:621	±	621:621	±	621:621	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	4	19	dep	HL	822:823	arg1	fat					830:832	17% fat	826:832	HL; 17% fat; 44% lactose	822:845	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	4	19	dep	HL	822:823	arg1	lactose					839:845	44% lactose	835:845	HL; 17% fat; 44% lactose	822:845	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	0	20	from	intake	83:88	arg1	calves					115:120	dairy calves	109:120	dairy calves	109:120	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	8	21	dep	libitum	1470:1476	arg1	feed					1490:1493	starter feed	1482:1493	starter feed	1482:1493	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	8	21	dep	libitum	1470:1476	arg1	straw					1510:1514	chopped wheat straw	1496:1514	chopped wheat straw	1496:1514	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	8	21	dep	libitum	1470:1476	arg1	water					1521:1525	water	1521:1525	water	1521:1525	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	8	21	dep	libitum	1470:1476	arg1	MR					1478:1479	MR	1478:1479	MR	1478:1479	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	2	22	theme	lactose	471:477	arg1	effects					439:445	the effects	435:445	the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior	435:559	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	3	23	theme	46.4	616:619	arg1	±					621:621	±	621:621	±	621:621	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	1	24	theme	milk	169:172	arg1	MR					185:186	MR	185:186	MR	185:186	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	1	24	theme	milk	169:172	arg1	replacers					174:182	commercial milk replacers	158:182	commercial milk replacers (MR) for calves	158:198	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	0	25	from	replacer	55:62	arg1	Effect					0:5	Effect	0:5	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.	0:121	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	3	26	theme	weight	639:644	arg1	kg					628:629	46.4 ± 0.77 kg	616:629	46.4 ± 0.77 kg of body weight	616:644	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	3	26	theme	weight	639:644	arg1	d					606:606	2.1 ± 0.16 d	595:606	2.1 ± 0.16 d of age	595:613	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	1	27	theme	lower	322:326	arg1	density					335:341	a lower energy density	320:341	a lower energy density	320:341	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	10	28	theme	body	1770:1773	arg1	measurements					1775:1786	body measurements	1770:1786	body measurements	1770:1786	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	11	29	theme	=	1907:1907	arg1	HL					1904:1905	HL	1904:1905	HL = 1.32 ± 0.04	1904:1919	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	5	30	from	difference	971:980	arg1	density					1006:1012	metabolizable energy density	985:1012	metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d)	985:1152	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	16	31	theme	significant	2760:2770	arg1	effects					2772:2778	significant effects	2760:2778	significant effects on solid feed intake and overall growth	2760:2818	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	4	32	dep	treatments	791:800	arg1	MR					818:819	a high-lactose MR	803:819	a high-lactose MR (HL; 17% fat; 44% lactose)	803:846	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	4	32	dep	treatments	791:800	arg1	MR					863:864	a high-fat MR	852:864	a high-fat MR (HF; 23% fat; 37% lactose)	852:891	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	4	32	dep	treatments	791:800	arg1	treatments					791:800	2 treatments	789:800	2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose)	789:891	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	14	33	theme	diet	2583:2586	arg1	consequence					2564:2574	a consequence	2562:2574	a consequence of the diet	2562:2586	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	14	34	theme	fatty	2515:2519	arg1	acids					2521:2525	nonesterified fatty acids	2501:2525	nonesterified fatty acids	2501:2525	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	3	35	theme	per	690:692	arg1	block					694:698	2 calves per block	681:698	2 calves per block based on arrival date and serum IgG	681:734	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	13	36	theme	±	2297:2297	arg1	g/d					2304:2306	352.1 ± 11.2 vs. 319.6 ± 11.6 g/d	2274:2306	g/d	2304:2306	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	6	37	theme	MR	1235:1236	arg1	libitum					1241:1247	their respective MR ad libitum	1218:1247	their respective MR ad libitum through teat buckets	1218:1268	For the first 2 wk of P1, calves were individually housed, fed their respective MR ad libitum through teat buckets, and provided access to water.					
33685703	14	38	theme	nonesterified	2501:2513	arg1	acids					2521:2525	nonesterified fatty acids	2501:2525	nonesterified fatty acids	2501:2525	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	1	39	theme	energy-to-protein	347:363	arg1	ratio					365:369	energy-to-protein ratio	347:369	energy-to-protein ratio	347:369	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	11	40	dep	=	1907:1907	arg1	±					1914:1914	1.32 ± 0.04	1909:1919	1.32 ± 0.04	1909:1919	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	10	41	theme	biweekly	1793:1800	arg1	samples					1808:1814	biweekly blood samples	1793:1814	biweekly blood samples	1793:1814	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	13	42	theme	protein-to-energy	2414:2430	arg1	ratio					2432:2436	protein-to-energy ratio	2414:2436	protein-to-energy ratio	2414:2436	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	5	43	theme	%	969:969	arg1	difference					971:980	a 6% difference	966:980	a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d)	966:1152	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	14	44	theme	Plasma	2478:2483	arg1	cholesterol					2485:2495	Plasma cholesterol	2478:2495	Plasma cholesterol	2478:2495	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	4	45	theme	37	880:881	arg1	%					882:882	%	882:882	%	882:882	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	6	46	theme	respective	1224:1233	arg1	libitum					1241:1247	their respective MR ad libitum	1218:1247	their respective MR ad libitum through teat buckets	1218:1268	For the first 2 wk of P1, calves were individually housed, fed their respective MR ad libitum through teat buckets, and provided access to water.					
33685703	13	47	theme	HL	2253:2254	arg1	calves					2256:2261	HL calves	2253:2261	HL calves	2253:2261	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	12	48	theme	milk	2137:2140	arg1	feeder					2142:2147	the automatic milk feeder	2123:2147	the automatic milk feeder than HF calves	2123:2162	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	0	49	from	Effect	0:5	arg1	replacer					55:62	milk replacer	50:62	milk replacer	50:62	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	0	49	from	Effect	0:5	arg1	performance					94:104	performance	94:104	performance in dairy calves	94:120	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	0	49	from	Effect	0:5	arg1	intake					83:88	ad libitum feed intake	67:88	ad libitum feed intake	67:88	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	0	50	theme	ad	67:68	arg1	intake					83:88	ad libitum feed intake	67:88	ad libitum feed intake	67:88	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	11	51	dep	=	1925:1925	arg1	HL					1904:1905	HL	1904:1905	HL = 1.32 ± 0.04	1904:1919	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	0	52	theme	feed	78:81	arg1	intake					83:88	ad libitum feed intake	67:88	ad libitum feed intake	67:88	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	7	53	theme	study	1425:1429	arg1	remainder					1408:1416	the remainder	1404:1416	the remainder of the study	1404:1429	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	3	54	dep	arrival	709:715	arg1	date					717:720	date	717:720	date	717:720	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	13	55	theme	energy	2396:2401	arg1	intake					2403:2408	metabolizable energy intake	2382:2408	metabolizable energy intake	2382:2408	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	4	56	theme	%	828:828	arg1	fat					830:832	17% fat	826:832	HL; 17% fat; 44% lactose	822:845	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	16	57	theme	solid	2783:2787	arg1	intake					2794:2799	solid feed intake	2783:2799	solid feed intake	2783:2799	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	0	58	theme	partial	10:16	arg1	exchange					18:25	partial exchange	10:25	partial exchange of lactose with fat in milk replacer	10:62	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	13	59	theme	protein	2223:2229	arg1	intake					2231:2236	Crude protein intake	2217:2236	Crude protein intake	2217:2236	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	5	60	dep	MR.	1030:1032	arg1	divided					1053:1059	divided	1053:1059	was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d)	1049:1152	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	4	61	theme	high-lactose	805:816	arg1	MR					818:819	a high-lactose MR	803:819	a high-lactose MR (HL; 17% fat; 44% lactose)	803:846	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	4	61	theme	high-lactose	805:816	arg1	treatments					791:800	2 treatments	789:800	2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose)	789:891	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	12	62	contain	have	2092:2095	arg2	visits					2113:2118	more unrewarded visits	2097:2118	more unrewarded visits to the automatic milk feeder than HF calves	2097:2162	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	12	62	contain	have	2092:2095	arg1	calves					2068:2073	HL calves	2065:2073	HL calves	2065:2073	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	5	63	theme	energy	999:1004	arg1	density					1006:1012	metabolizable energy density	985:1012	metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d)	985:1152	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	8	64	theme	chopped	1496:1502	arg1	straw					1510:1514	chopped wheat straw	1496:1514	chopped wheat straw	1496:1514	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	3	65	dep	d	606:606	arg1	BW					647:648	BW	647:648	BW	647:648	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	12	66	theme	HF	2154:2155	arg1	calves					2157:2162	HF calves	2154:2162	HF calves	2154:2162	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	1	67	theme	fat	260:262	arg1	levels					250:255	low levels	246:255	low levels of fat	246:262	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	1	67	theme	fat	260:262	arg1	levels					224:229	relatively high levels	208:229	relatively high levels of lactose	208:240	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	13	68	theme	higher	2338:2343	arg1	intake					2345:2350	the higher intake	2334:2350	the higher intake of MR during that period	2334:2375	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	11	69	theme	starter	1980:1986	arg1	intake					1988:1993	total starter intake	1974:1993	total starter intake	1974:1993	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	16	70	theme	overall	2805:2811	arg1	growth					2813:2818	overall growth	2805:2818	overall growth	2805:2818	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	16	71	from	effects	2772:2778	arg1	intake					2794:2799	solid feed intake	2783:2799	solid feed intake	2783:2799	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	16	71	from	effects	2772:2778	arg1	growth					2813:2818	overall growth	2805:2818	overall growth	2805:2818	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	7	72	from	d	1315:1315	arg1	blocks/pen					1353:1362	4 blocks/pen	1351:1362	4 blocks/pen	1351:1362	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	7	72	from	d	1315:1315	arg1	calves					1367:1372	8 calves	1365:1372	8 calves	1365:1372	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	3	73	theme	male	573:576	arg1	d					606:606	2.1 ± 0.16 d	595:606	2.1 ± 0.16 d of age	595:613	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	3	73	theme	male	573:576	arg1	calves					587:592	Thirty-two male Holstein calves	562:592	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW)	562:649	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	2	74	theme	feed	507:510	arg1	intake					512:517	voluntary feed intake	497:517	voluntary feed intake	497:517	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	15	75	theme	final	2603:2607	arg1	d					2616:2616	84 d	2613:2616	84 d	2613:2616	Nevertheless, final BW (84 d) did not differ between treatments.					
33685703	15	75	theme	final	2603:2607	arg1	BW					2609:2610	final BW	2603:2610	final BW (84 d)	2603:2617	Nevertheless, final BW (84 d) did not differ between treatments.					
33685703	4	76	theme	%	837:837	arg1	lactose					839:845	44% lactose	835:845	HL; 17% fat; 44% lactose	822:845	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	1	77	theme	high	219:222	arg1	levels					224:229	relatively high levels	208:229	relatively high levels of lactose	208:240	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	10	78	theme	daily	1687:1691	arg1	intakes					1693:1699	daily intakes	1687:1699	daily intakes	1687:1699	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	10	78	theme	daily	1687:1691	arg1	visits					1747:1752	rewarded and unrewarded visits	1723:1752	rewarded and unrewarded visits	1723:1752	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	11	79	theme	dry	1945:1947	arg1	matter					1949:1954	dry matter	1945:1954	dry matter	1945:1954	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	1	80	theme	Holstein	137:144	arg1	milk					152:155	Holstein whole milk	137:155	Holstein whole milk	137:155	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	10	81	theme	rewarded	1723:1730	arg1	visits					1747:1752	rewarded and unrewarded visits	1723:1752	rewarded and unrewarded visits	1723:1752	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	10	81	theme	rewarded	1723:1730	arg1	intakes					1693:1699	daily intakes	1687:1699	daily intakes	1687:1699	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	7	82	theme	0.5	1311:1313	arg1	±					1309:1309	±	1309:1309	±	1309:1309	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	4	83	theme	%	873:873	arg1	fat					875:877	23% fat	871:877	HF; 23% fat; 37% lactose	867:890	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	9	84	theme	milk	1605:1608	arg1	withdrawal					1610:1619	complete milk withdrawal	1596:1619	complete milk withdrawal by 57 d	1596:1627	During P2, calves were gradually weaned until complete milk withdrawal by 57 d and then monitored until 84 d (P3).					
33685703	7	85	theme	14.2	1304:1307	arg1	±					1309:1309	±	1309:1309	±	1309:1309	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	4	86	theme	high-fat	854:861	arg1	MR					863:864	a high-fat MR	852:864	a high-fat MR (HF; 23% fat; 37% lactose)	852:891	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	4	86	theme	high-fat	854:861	arg1	treatments					791:800	2 treatments	789:800	2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose)	789:891	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	16	87	theme	energy	2736:2741	arg1	density					2743:2749	its energy density	2732:2749	its energy density	2732:2749	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	1	88	theme	commercial	158:167	arg1	MR					185:186	MR	185:186	MR	185:186	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	1	88	theme	commercial	158:167	arg1	replacers					174:182	commercial milk replacers	158:182	commercial milk replacers (MR) for calves	158:198	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	2	89	theme	exchanging	460:469	arg1	lactose					471:477	partially exchanging lactose	450:477	partially exchanging lactose with fat in MR	450:492	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	0	90	theme	dairy	109:113	arg1	calves					115:120	dairy calves	109:120	dairy calves	109:120	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	5	91	dep	P2	1110:1111	arg1	d					1120:1120	36-56 d	1114:1120	P2; 36-56 d	1110:1120	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	10	92	theme	unrewarded	1736:1745	arg1	visits					1747:1752	rewarded and unrewarded visits	1723:1752	rewarded and unrewarded visits	1723:1752	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	10	92	theme	unrewarded	1736:1745	arg1	intakes					1693:1699	daily intakes	1687:1699	daily intakes	1687:1699	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	16	93	theme	ad	2674:2675	arg1	libitum					2677:2683	ad libitum	2674:2683	ad libitum	2674:2683	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	8	94	theme	ad	1467:1468	arg1	libitum					1470:1476	ad libitum MR, starter feed, chopped wheat straw, and water	1467:1525	ad libitum MR, starter feed, chopped wheat straw, and water	1467:1525	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	1	95	theme	energy	328:333	arg1	density					335:341	a lower energy density	320:341	a lower energy density	320:341	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	4	96	dep	MR	863:864	arg1	HF					867:868	HF	867:868	HF; 23% fat; 37% lactose	867:890	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	5	97	dep	weaning	1101:1107	arg1	P2					1110:1111	P2	1110:1111	P2; 36-56 d	1110:1120	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	11	98	dep	%	1901:1901	arg1	=					1925:1925	=	1925:1925	=	1925:1925	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	6	99	theme	ad	1238:1239	arg1	libitum					1241:1247	their respective MR ad libitum	1218:1247	their respective MR ad libitum through teat buckets	1218:1268	For the first 2 wk of P1, calves were individually housed, fed their respective MR ad libitum through teat buckets, and provided access to water.					
33685703	11	100	theme	fat	1828:1830	arg1	content					1832:1838	fat content	1828:1838	fat content	1828:1838	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	11	101	theme	MR	2015:2016	arg1	composition					2018:2028	MR composition	2015:2028	MR composition	2015:2028	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	3	102	theme	body	634:637	arg1	weight					639:644	body weight	634:644	body weight	634:644	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	8	103	theme	group	1439:1443	arg1	pens					1445:1448	the group pens	1435:1448	the group pens	1435:1448	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	6	104	theme	first	1163:1167	arg1	wk					1171:1172	the first 2 wk	1159:1172	the first 2 wk of P1	1159:1178	For the first 2 wk of P1, calves were individually housed, fed their respective MR ad libitum through teat buckets, and provided access to water.					
33685703	3	105	theme	block	694:698	arg1	blocks					671:676	16 blocks	668:676	16 blocks of 2 calves per block based on arrival date and serum IgG	668:734	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	10	106	theme	weekly	1756:1761	arg1	BW					1763:1764	weekly BW	1756:1764	weekly BW	1756:1764	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	2	107	from	effects	439:445	arg1	behavior					552:559	feeding behavior	544:559	feeding behavior	544:559	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	2	107	from	effects	439:445	arg1	intake					512:517	voluntary feed intake	497:517	voluntary feed intake	497:517	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	2	107	from	effects	439:445	arg1	performance					527:537	growth performance	520:537	growth performance	520:537	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	2	107	from	effects	439:445	arg1	MR					491:492	MR	491:492	MR	491:492	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	11	108	theme	lactose	1858:1864	arg1	expense					1847:1853	the expense	1843:1853	the expense of lactose	1843:1864	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	5	109	theme	6	968:968	arg1	%					969:969	%	969:969	%	969:969	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	0	110	theme	milk	50:53	arg1	replacer					55:62	milk replacer	50:62	milk replacer	50:62	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	14	111	theme	cholesterol	2485:2495	arg1	levels					2527:2532	Plasma cholesterol and nonesterified fatty acids levels	2478:2532	Plasma cholesterol and nonesterified fatty acids levels	2478:2532	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	3	112	theme	calves	683:688	arg1	block					694:698	2 calves per block	681:698	2 calves per block based on arrival date and serum IgG	681:734	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	2	113	theme	study	412:416	arg1	objective					394:402	the objective	390:402	the objective of this study	390:416	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	0	114	from	exchange	18:25	arg1	replacer					55:62	milk replacer	50:62	milk replacer	50:62	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	4	115	theme	%	882:882	arg1	lactose					884:890	37% lactose	880:890	HF; 23% fat; 37% lactose	867:890	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	5	116	theme	weight	939:944	arg1	basis					946:950	weight basis	939:950	weight basis	939:950	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	1	117	theme	protein	269:275	arg1	levels					277:282	protein levels	269:282	protein levels	269:282	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	4	118	dep	HF	867:868	arg1	fat					875:877	23% fat	871:877	HF; 23% fat; 37% lactose	867:890	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	4	118	dep	HF	867:868	arg1	lactose					884:890	37% lactose	880:890	HF; 23% fat; 37% lactose	867:890	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	5	119	dep	P1	1088:1089	arg1	d					1097:1097	0-35 d	1092:1097	P1; 0-35 d	1088:1097	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	11	120	theme	MR	1876:1877	arg1	intake					1879:1884	MR intake	1876:1884	MR intake	1876:1884	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	16	121	theme	MR	2720:2721	arg1	intake					2710:2715	their intake	2704:2715	their intake of MR	2704:2721	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	0	122	theme	libitum	70:76	arg1	intake					83:88	ad libitum feed intake	67:88	ad libitum feed intake	67:88	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	5	123	dep	preweaning	1076:1085	arg1	P1					1088:1089	P1	1088:1089	P1; 0-35 d	1088:1097	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	12	124	theme	automatic	2127:2135	arg1	feeder					2142:2147	the automatic milk feeder	2123:2147	the automatic milk feeder than HF calves	2123:2162	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	14	125	theme	HF	2549:2550	arg1	calves					2552:2557	HF calves	2549:2557	HF calves	2549:2557	Plasma cholesterol and nonesterified fatty acids levels were higher in HF calves as a consequence of the diet.					
33685703	2	126	theme	feeding	544:550	arg1	behavior					552:559	feeding behavior	544:559	feeding behavior	544:559	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	5	127	dep	phases	1068:1073	arg1	postweaning					1128:1138	postweaning	1128:1138	postweaning	1128:1138	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	5	127	dep	phases	1068:1073	arg1	preweaning					1076:1085	preweaning	1076:1085	preweaning	1076:1085	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	5	127	dep	phases	1068:1073	arg1	weaning					1101:1107	weaning	1101:1107	weaning	1101:1107	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	10	128	theme	blood	1802:1806	arg1	samples					1808:1814	biweekly blood samples	1793:1814	biweekly blood samples	1793:1814	Measurements included daily intakes and feeding behavior (rewarded and unrewarded visits), weekly BW and body measurements, and biweekly blood samples.					
33685703	8	129	theme	starter	1482:1488	arg1	feed					1490:1493	starter feed	1482:1493	starter feed	1482:1493	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	3	130	theme	serum	726:730	arg1	IgG					732:734	serum IgG	726:734	serum IgG	726:734	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	8	131	theme	automated	1531:1539	arg1	feeders					1541:1547	automated feeders	1531:1547	automated feeders	1531:1547	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	12	132	theme	unrewarded	2102:2111	arg1	visits					2113:2118	more unrewarded visits	2097:2118	more unrewarded visits to the automatic milk feeder than HF calves	2097:2162	Once MR was restricted during P2, HL calves were reported to have more unrewarded visits to the automatic milk feeder than HF calves (11.9 ± 0.95 vs. 8.4 ± 1.03 visits/d, respectively).					
33685703	6	133	theme	teat	1257:1260	arg1	buckets					1262:1268	teat buckets	1257:1268	teat buckets	1257:1268	For the first 2 wk of P1, calves were individually housed, fed their respective MR ad libitum through teat buckets, and provided access to water.					
33685703	0	134	theme	exchange	18:25	arg1	Effect					0:5	Effect	0:5	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.	0:121	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	1	135	theme	lactose	234:240	arg1	levels					250:255	low levels	246:255	low levels of fat	246:262	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	1	135	theme	lactose	234:240	arg1	levels					224:229	relatively high levels	208:229	relatively high levels of lactose	208:240	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	6	136	theme	P1	1177:1178	arg1	wk					1171:1172	the first 2 wk	1159:1172	the first 2 wk of P1	1159:1178	For the first 2 wk of P1, calves were individually housed, fed their respective MR ad libitum through teat buckets, and provided access to water.					
33685703	13	137	theme	MR	2355:2356	arg1	intake					2345:2350	the higher intake	2334:2350	the higher intake of MR during that period	2334:2375	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	4	138	theme	17	826:827	arg1	%					828:828	%	828:828	%	828:828	Within each block, calves were randomly assigned to 2 treatments: a high-lactose MR (HL; 17% fat; 44% lactose), or a high-fat MR (HF; 23% fat; 37% lactose).					
33685703	5	139	theme	metabolizable	985:997	arg1	density					1006:1012	metabolizable energy density	985:1012	metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d)	985:1152	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	0	140	theme	lactose	30:36	arg1	exchange					18:25	partial exchange	10:25	partial exchange of lactose with fat in milk replacer	10:62	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	1	141	theme	low	246:248	arg1	levels					250:255	low levels	246:255	low levels of fat	246:262	Compared with Holstein whole milk, commercial milk replacers (MR) for calves deliver relatively high levels of lactose and low levels of fat, and protein levels are rather comparable, resulting in a lower energy density and energy-to-protein ratio of the diet.					
33685703	7	142	theme	group	1389:1393	arg1	pens					1395:1398	group pens	1389:1398	group pens	1389:1398	At 14.2 ± 0.5 d of age, calves were group-housed (4 blocks/pen, 8 calves) and housed in group pens for the remainder of the study.					
33685703	13	143	dep	P1	2270:2271	arg1	g/d					2304:2306	352.1 ± 11.2 vs. 319.6 ± 11.6 g/d	2274:2306	g/d	2304:2306	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	13	143	dep	P1	2270:2271	arg1	±					2280:2280	352.1 ± 11.2 vs. 319.6 ± 11.6 g/d	2274:2306	±	2280:2280	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	0	144	from	performance	94:104	arg1	calves					115:120	dairy calves	109:120	dairy calves	109:120	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	0	145	with	exchange	18:25	arg1	fat					43:45	fat	43:45	fat	43:45	Effect of partial exchange of lactose with fat in milk replacer on ad libitum feed intake and performance in dairy calves.					
33685703	8	146	theme	wheat	1504:1508	arg1	straw					1510:1514	chopped wheat straw	1496:1514	chopped wheat straw	1496:1514	In the group pens, calves were fed ad libitum MR, starter feed, chopped wheat straw, and water via automated feeders.					
33685703	11	147	theme	total	1974:1978	arg1	intake					1988:1993	total starter intake	1974:1993	total starter intake	1974:1993	Increasing fat content at the expense of lactose decreased MR intake during P1 by 15% (HL = 1.32 ± 0.04; HF = 1.17 ± 0.04 kg of dry matter per day), whereas total starter intake was not affected by MR composition.					
33685703	3	148	theme	Holstein	578:585	arg1	d					606:606	2.1 ± 0.16 d	595:606	2.1 ± 0.16 d of age	595:613	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	3	148	theme	Holstein	578:585	arg1	calves					587:592	Thirty-two male Holstein calves	562:592	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW)	562:649	Thirty-two male Holstein calves (2.1 ± 0.16 d of age, 46.4 ± 0.77 kg of body weight; BW) were assigned to 16 blocks of 2 calves per block based on arrival date and serum IgG.					
33685703	16	149	theme	feed	2789:2792	arg1	intake					2794:2799	solid feed intake	2783:2799	solid feed intake	2783:2799	Overall, calves fed ad libitum seemed to regulate their intake of MR based on its energy density, without significant effects on solid feed intake and overall growth.					
33685703	5	150	theme	MR.	1030:1032	arg1	kilogram					1018:1025	kilogram	1018:1025	kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d)	1018:1152	Lactose was exchanged by fat on a weight per weight basis, resulting in a 6% difference in metabolizable energy density per kilogram of MR. The experiment was divided into 3 phases: preweaning (P1; 0-35 d), weaning (P2; 36-56 d), and postweaning (P3; 57-84 d).					
33685703	2	151	with	lactose	471:477	arg1	fat					484:486	fat	484:486	fat	484:486	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33685703	13	152	theme	Crude	2217:2221	arg1	intake					2231:2236	Crude protein intake	2217:2236	Crude protein intake	2217:2236	Crude protein intake was higher for HL calves during P1 (352.1 ± 11.2 vs. 319.6 ± 11.6 g/d), which was attributed to the higher intake of MR during that period, and metabolizable energy intake and protein-to-energy ratio remained comparable between treatments.					
33685703	2	153	from	lactose	471:477	arg1	MR					491:492	MR	491:492	MR	491:492	Thus, the objective of this study was to determine the effects of partially exchanging lactose with fat in MR on voluntary feed intake, growth performance, and feeding behavior.					
33436252	0	0	theme	thermal	62:68	arg1	kinetics					83:90	thermal inactivation kinetics	62:90	thermal inactivation kinetics of barley malt α-amylase and β-amylase	62:129	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	1	1	from	key	156:158	arg1	processes					182:190	several industrial processes	163:190	several industrial processes	163:190	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	1	1	from	key	156:158	arg1	brewing					203:209	brewing	203:209	brewing	203:209	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	5	2	theme	Thermal	706:712	arg1	kinetics					727:734	Thermal inactivation kinetics	706:734	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system	706:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	1	3	theme	starch	146:151	arg1	Hydrolysis					132:141	Hydrolysis	132:141	Hydrolysis of starch	132:151	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	4	4	with	°C	654:655	arg1	heating					685:691	intermediate heating	672:691	intermediate heating by 1 °C/min	672:703	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	5	5	from	variety	813:819	arg1	system					834:839	a diluted system	824:839	a diluted system	824:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	4	6	theme	wort	569:572	arg1	composition					574:584	the resulting wort composition	555:584	the resulting wort composition after mashing	555:598	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	0	7	theme	inactivation	70:81	arg1	kinetics					83:90	thermal inactivation kinetics	62:90	thermal inactivation kinetics of barley malt α-amylase and β-amylase	62:129	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	2	8	theme	amylases	260:267	arg1	activity					222:229	activity	222:229	activity	222:229	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	2	8	theme	amylases	260:267	arg1	kinetics					248:255	inactivation kinetics	235:255	inactivation kinetics	235:255	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	2	8	theme	amylases	260:267	arg1	prerequisite					323:334	a prerequisite	321:334	a prerequisite for rational optimisation of this process	321:376	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	4	9	theme	resulting	559:567	arg1	composition					574:584	the resulting wort composition	555:584	the resulting wort composition after mashing	555:598	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	0	10	theme	barley	95:100	arg1	α-amylase					107:115	barley malt α-amylase	95:115	barley malt α-amylase	95:115	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	5	11	from	β-amylases	769:778	arg1	system					834:839	a diluted system	824:839	a diluted system	824:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	5	12	from	system	834:839	arg1	α-					762:763	α-	762:763	α-	762:763	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	5	12	from	system	834:839	arg1	β-amylases					769:778	β-amylases	769:778	β-amylases	769:778	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	3	13	theme	malt	499:502	arg1	varieties					504:512	six barley and malt varieties	484:512	varieties	504:512	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	2	14	dep	activity	222:229	arg1	the					218:220	the	218:220	the	218:220	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	5	15	theme	lower	881:885	arg1	temperatures					887:898	lower temperatures	881:898	lower temperatures than expected	881:912	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	6	16	theme	β-amylase	1047:1055	arg1	stability					1057:1065	β-amylase stability	1047:1065	β-amylase stability	1047:1065	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	2	17	theme	process	370:376	arg1	optimisation					349:360	rational optimisation	340:360	rational optimisation of this process	340:376	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	5	18	from	α-	762:763	arg1	system					834:839	a diluted system	824:839	a diluted system	824:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	4	19	theme	intermediate	672:683	arg1	heating					685:691	intermediate heating	672:691	intermediate heating by 1 °C/min	672:703	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	0	20	theme	Starch	0:5	arg1	hydrolysis					7:16	Starch hydrolysis	0:16	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.	0:130	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	6	21	theme	kinetic	928:934	arg1	parameters					936:945	The obtained kinetic parameters	915:945	The obtained kinetic parameters	915:945	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	5	22	theme	malt	808:811	arg1	variety					813:819	an industrially relevant malt variety	783:819	an industrially relevant malt variety in a diluted system	783:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	6	23	theme	formed	1119:1124	arg1	components					1097:1106	components	1097:1106	components present or formed in the mash	1097:1136	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	1	24	theme	several	163:169	arg1	processes					182:190	several industrial processes	163:190	several industrial processes	163:190	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	1	24	theme	several	163:169	arg1	brewing					203:209	brewing	203:209	brewing	203:209	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	6	25	theme	obtained	919:926	arg1	parameters					936:945	The obtained kinetic parameters	915:945	The obtained kinetic parameters	915:945	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	0	26	theme	α-amylase	107:115	arg1	kinetics					83:90	thermal inactivation kinetics	62:90	thermal inactivation kinetics of barley malt α-amylase and β-amylase	62:129	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	0	26	theme	α-amylase	107:115	arg1	study					36:40	A study	34:40	A study of the activity	34:56	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	6	27	theme	present	1108:1114	arg1	components					1097:1106	components	1097:1106	components present or formed in the mash	1097:1136	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	2	28	theme	malt	287:290	arg1	mashing					292:298	barley malt mashing	280:298	barley malt mashing	280:298	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	1	29	theme	industrial	171:180	arg1	processes					182:190	several industrial processes	163:190	several industrial processes	163:190	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	1	29	theme	industrial	171:180	arg1	brewing					203:209	brewing	203:209	brewing	203:209	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	0	30	theme	malt	102:105	arg1	α-amylase					107:115	barley malt α-amylase	95:115	barley malt α-amylase	95:115	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	3	31	located	observed	405:412	arg1	activity					421:428	the activity	417:428	the activity of α- and β-amylases	417:449	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	3	31	located	observed	405:412	arg2	differences					388:398	Varietal differences	379:398	Varietal differences	379:398	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	3	31	located	observed	405:412	arg2	function					456:463	a function	454:463	a function of temperature for six barley and malt varieties	454:512	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	2	32	theme	barley	280:285	arg1	mashing					292:298	barley malt mashing	280:298	barley malt mashing	280:298	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	5	33	theme	diluted	826:832	arg1	system					834:839	a diluted system	824:839	a diluted system	824:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	5	34	theme	inactivation	714:725	arg1	kinetics					727:734	Thermal inactivation kinetics	706:734	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system	706:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	0	35	theme	β-amylase	121:129	arg1	kinetics					83:90	thermal inactivation kinetics	62:90	thermal inactivation kinetics of barley malt α-amylase and β-amylase	62:129	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	0	35	theme	β-amylase	121:129	arg1	study					36:40	A study	34:40	A study of the activity	34:56	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	4	36	theme	min	637:639	arg1	phases					624:629	three isothermal phases	607:629	three isothermal phases of 30 min	607:639	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	3	37	theme	α-	433:434	arg1	activity					421:428	the activity	417:428	the activity of α- and β-amylases	417:449	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	5	38	theme	kinetics	727:734	arg1	parameters					736:745	Thermal inactivation kinetics parameters	706:745	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system	706:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	6	39	theme	real	1006:1009	arg1	conditions					1019:1028	real mashing conditions	1006:1028	real mashing conditions	1006:1028	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	4	40	theme	isothermal	613:622	arg1	phases					624:629	three isothermal phases	607:629	three isothermal phases of 30 min	607:639	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	3	41	theme	Varietal	379:386	arg1	differences					388:398	Varietal differences	379:398	Varietal differences	379:398	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	3	41	theme	Varietal	379:386	arg1	function					456:463	a function	454:463	a function of temperature for six barley and malt varieties	454:512	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	5	42	dep	temperatures	887:898	arg1	expected					905:912	expected	905:912	expected	905:912	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	3	43	theme	temperature	468:478	arg1	differences					388:398	Varietal differences	379:398	Varietal differences	379:398	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	3	43	theme	temperature	468:478	arg1	function					456:463	a function	454:463	a function of temperature for six barley and malt varieties	454:512	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	2	44	theme	inactivation	235:246	arg1	activity					222:229	activity	222:229	activity	222:229	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	2	44	theme	inactivation	235:246	arg1	kinetics					248:255	inactivation kinetics	235:255	inactivation kinetics	235:255	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	2	44	theme	inactivation	235:246	arg1	prerequisite					323:334	a prerequisite	321:334	a prerequisite for rational optimisation of this process	321:376	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
33436252	1	45	from	processes	182:190	arg1	key					156:158	key	156:158	key	156:158	Hydrolysis of starch is key in several industrial processes, including brewing.					
33436252	6	46	theme	β-amylase	980:988	arg1	inactivation					990:1001	β-amylase inactivation	980:1001	β-amylase inactivation	980:1001	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	6	47	theme	mashing	1011:1017	arg1	conditions					1019:1028	real mashing conditions	1006:1028	real mashing conditions	1006:1028	The obtained kinetic parameters could predict α-amylase, but not β-amylase inactivation in real mashing conditions, suggesting that β-amylase stability is enhanced during mashing by components present or formed in the mash.					
33436252	0	48	theme	activity	49:56	arg1	kinetics					83:90	thermal inactivation kinetics	62:90	thermal inactivation kinetics of barley malt α-amylase and β-amylase	62:129	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	0	48	theme	activity	49:56	arg1	study					36:40	A study	34:40	A study of the activity	34:56	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	5	49	theme	relevant	799:806	arg1	variety					813:819	an industrially relevant malt variety	783:819	an industrially relevant malt variety in a diluted system	783:839	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	0	50	dep	hydrolysis	7:16	arg1	kinetics					83:90	thermal inactivation kinetics	62:90	thermal inactivation kinetics of barley malt α-amylase and β-amylase	62:129	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	0	50	dep	hydrolysis	7:16	arg1	study					36:40	A study	34:40	A study of the activity	34:56	Starch hydrolysis during mashing: A study of the activity and thermal inactivation kinetics of barley malt α-amylase and β-amylase.					
33436252	3	51	theme	β-amylases	440:449	arg1	activity					421:428	the activity	417:428	the activity of α- and β-amylases	417:449	Varietal differences were observed in the activity of α- and β-amylases as a function of temperature for six barley and malt varieties.					
33436252	4	52	theme	°C	647:648	arg1	°C					654:655	45 °C, 62 °C and 72 °C	644:665	°C	654:655	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	5	53	theme	variety	813:819	arg1	α-					762:763	α-	762:763	α-	762:763	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	5	53	theme	variety	813:819	arg1	β-amylases					769:778	β-amylases	769:778	β-amylases	769:778	Thermal inactivation kinetics parameters determined for α- and β-amylases of an industrially relevant malt variety in a diluted system showed that enzymes were inactivated at lower temperatures than expected.					
33436252	4	54	with	°C	664:665	arg1	heating					685:691	intermediate heating	672:691	intermediate heating by 1 °C/min	672:703	These differences were not reflected in the resulting wort composition after mashing, using three isothermal phases of 30 min at 45 °C, 62 °C and 72 °C with intermediate heating by 1 °C/min.					
33436252	2	55	theme	rational	340:347	arg1	optimisation					349:360	rational optimisation	340:360	rational optimisation of this process	340:376	Here, the activity and inactivation kinetics of amylases throughout barley malt mashing are investigated, as a prerequisite for rational optimisation of this process.					
34564432	5	0	theme	major	597:601	arg1	isofom					603:608	a major isofom	595:608	a major isofom	595:608	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	5	0	theme	major	597:601	arg1	Man-Tf					585:590	Man-Tf	585:590	Man-Tf	585:590	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	2	1	theme	cerebrospinal	325:337	arg1	CSF					346:348	CSF	346:348	CSF	346:348	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	2	1	theme	cerebrospinal	325:337	arg1	fluid					339:343	cerebrospinal fluid	325:343	cerebrospinal fluid (CSF)	325:349	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	7	2	theme	mild	823:826	arg1	impairment					838:847	mild cognitive impairment	823:847	mild cognitive impairment	823:847	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	9	3	theme	%	1269:1269	arg1	sensitivities					1271:1283	84% sensitivities	1267:1283	84% sensitivities	1267:1283	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	4	4	theme	unique	484:489	arg1	mannosylated-glycan					491:509	a unique mannosylated-glycan	482:509	a unique mannosylated-glycan	482:509	We found that a unique mannosylated-glycan is carried by a Tf isoform in CSF (Man-Tf).					
34564432	6	5	theme	neurological	736:747	arg1	diseases					749:756	neurological diseases	736:756	neurological diseases	736:756	Man-Tf levels were analyzed in CSF of patients with neurological diseases.					
34564432	2	6	from	disease	309:315	arg1	CSF					346:348	CSF	346:348	CSF	346:348	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	2	6	from	disease	309:315	arg1	fluid					339:343	cerebrospinal fluid	325:343	cerebrospinal fluid (CSF)	325:349	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	8	7	theme	hippocampal	1067:1077	arg1	neurons					1079:1085	hippocampal neurons	1067:1085	hippocampal neurons of AD	1067:1091	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	5	8	theme	cerebral	559:566	arg1	cortex					568:573	The cerebral cortex	555:573	The cerebral cortex	555:573	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	5	9	contain	contained	575:583	arg2	isofom					603:608	a major isofom	595:608	a major isofom	595:608	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	5	9	contain	contained	575:583	arg2	Man-Tf					585:590	Man-Tf	585:590	Man-Tf	585:590	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	5	9	contain	contained	575:583	arg1	cortex					568:573	The cerebral cortex	555:573	The cerebral cortex	555:573	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	8	10	theme	AD	1090:1091	arg1	neurons					1079:1085	hippocampal neurons	1067:1085	hippocampal neurons of AD	1067:1091	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	8	11	with	Consistent	1000:1009	arg1	observation					1020:1030	the observation	1016:1030	the observation	1016:1030	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	9	12	theme	%	1291:1291	arg1	specificities					1293:1305	90% specificities	1289:1305	90% specificities	1289:1305	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	9	13	theme	90	1289:1290	arg1	%					1291:1291	%	1291:1291	%	1291:1291	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	4	14	from	isoform	530:536	arg1	Man-Tf					546:551	Man-Tf	546:551	Man-Tf	546:551	We found that a unique mannosylated-glycan is carried by a Tf isoform in CSF (Man-Tf).					
34564432	4	14	from	isoform	530:536	arg1	CSF					541:543	CSF	541:543	CSF (Man-Tf)	541:552	We found that a unique mannosylated-glycan is carried by a Tf isoform in CSF (Man-Tf).					
34564432	7	15	theme	Man-Tf	777:782	arg1	Concentrations					759:772	Concentrations	759:772	Concentrations of Man-Tf	759:782	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	3	16	theme	ultra-performance	397:413	arg1	chromatography					422:435	ultra-performance liquid chromatography	397:435	ultra-performance liquid chromatography followed by mass spectrometry	397:465	Glycan structures of CSF Tf were analyzed by ultra-performance liquid chromatography followed by mass spectrometry.					
34564432	6	17	with	patients	722:729	arg1	diseases					749:756	neurological diseases	736:756	neurological diseases	736:756	Man-Tf levels were analyzed in CSF of patients with neurological diseases.					
34564432	9	18	theme	p-tau	1204:1208	arg1	levels					1194:1199	levels	1194:1199	levels of p-tau x Man-Tf	1194:1217	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	7	19	theme	other	870:874	arg1	diseases					889:896	other neurological diseases	870:896	other neurological diseases	870:896	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	1	20	theme	biomarker	177:185	arg1	identification					187:200	biomarker identification	177:200	biomarker identification in various diseases	177:220	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	3	21	theme	Glycan	352:357	arg1	structures					359:368	Glycan structures	352:368	Glycan structures of CSF Tf	352:378	Glycan structures of CSF Tf were analyzed by ultra-performance liquid chromatography followed by mass spectrometry.					
34564432	4	22	theme	Tf	527:528	arg1	isoform					530:536	a Tf isoform	525:536	a Tf isoform in CSF (Man-Tf)	525:552	We found that a unique mannosylated-glycan is carried by a Tf isoform in CSF (Man-Tf).					
34564432	8	23	theme	Man-Tf	1142:1147	arg1	p-tau					1132:1136	a combined p-tau	1121:1136	a combined p-tau	1121:1136	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	8	23	theme	Man-Tf	1142:1147	arg1	measure					1149:1155	Man-Tf measure	1142:1155	Man-Tf measure	1142:1155	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	8	23	theme	Man-Tf	1142:1147	arg1	biomarker					1168:1176	a biomarker	1166:1176	a biomarker for AD	1166:1183	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	7	24	theme	neurological	876:887	arg1	diseases					889:896	other neurological diseases	870:896	other neurological diseases	870:896	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	7	25	theme	AD	989:990	arg1	phosphorylated-tau					944:961	phosphorylated-tau	944:961	phosphorylated-tau (p-tau)	944:969	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	7	25	theme	AD	989:990	arg1	marker					992:997	a representative AD marker	972:997	a representative AD marker	972:997	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	9	26	theme	%	1343:1343	arg1	specificities					1345:1357	89% specificities	1341:1357	89% specificities for AD	1341:1364	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	2	27	from	glycan-biomarkers	255:271	arg1	Tf					289:290	Tf	289:290	Tf	289:290	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	2	27	from	glycan-biomarkers	255:271	arg1	transferrin					276:286	transferrin	276:286	transferrin (Tf)	276:291	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	1	28	theme	various	205:211	arg1	diseases					213:220	various diseases	205:220	various diseases	205:220	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	3	29	theme	CSF	373:375	arg1	Tf					377:378	CSF Tf	373:378	CSF Tf	373:378	Glycan structures of CSF Tf were analyzed by ultra-performance liquid chromatography followed by mass spectrometry.					
34564432	9	30	theme	high	1226:1229	arg1	accuracy					1242:1249	high diagnostic accuracy	1226:1249	high diagnostic accuracy for MCI and AD	1226:1264	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	9	31	theme	%	1321:1321	arg1	sensitivities					1323:1335	MCI and 94% sensitivities	1311:1335	MCI and 94% sensitivities	1311:1335	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	2	32	theme	study	235:239	arg1	Aim					223:225	Aim	223:225	Aim of this study	223:239	Aim of this study is to explore glycan-biomarkers on transferrin (Tf) for Alzheimer's disease (AD) in cerebrospinal fluid (CSF).					
34564432	9	33	theme	MCI	1311:1313	arg1	sensitivities					1323:1335	MCI and 94% sensitivities	1311:1335	MCI and 94% sensitivities	1311:1335	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	3	34	theme	liquid	415:420	arg1	chromatography					422:435	ultra-performance liquid chromatography	397:435	ultra-performance liquid chromatography followed by mass spectrometry	397:465	Glycan structures of CSF Tf were analyzed by ultra-performance liquid chromatography followed by mass spectrometry.					
34564432	3	35	theme	Tf	377:378	arg1	structures					359:368	Glycan structures	352:368	Glycan structures of CSF Tf	352:378	Glycan structures of CSF Tf were analyzed by ultra-performance liquid chromatography followed by mass spectrometry.					
34564432	9	36	theme	diagnostic	1231:1240	arg1	accuracy					1242:1249	high diagnostic accuracy	1226:1249	high diagnostic accuracy for MCI and AD	1226:1264	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	7	37	theme	representative	974:987	arg1	phosphorylated-tau					944:961	phosphorylated-tau	944:961	phosphorylated-tau (p-tau)	944:969	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	7	37	theme	representative	974:987	arg1	marker					992:997	a representative AD marker	972:997	a representative AD marker	972:997	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	10	38	theme	new	1390:1392	arg1	biomarker					1394:1402	a new biomarker	1388:1402	a new biomarker for AD	1388:1409	Thus Man-Tf could be a new biomarker for AD.					
34564432	10	38	theme	new	1390:1392	arg1	Man-Tf					1372:1377	Man-Tf	1372:1377	Man-Tf	1372:1377	Thus Man-Tf could be a new biomarker for AD.					
34564432	0	39	theme	Novel	45:49	arg1	Biomarker					51:59	a Novel Biomarker	43:59	a Novel Biomarker for Alzheimer's Disease	43:83	Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimer's Disease.					
34564432	0	39	theme	Novel	45:49	arg1	Transferrin					0:10	Transferrin	0:10	Transferrin Biosynthesized in the Brain	0:38	Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimer's Disease.					
34564432	6	40	theme	patients	722:729	arg1	CSF					715:717	CSF	715:717	CSF of patients with neurological diseases	715:756	Man-Tf levels were analyzed in CSF of patients with neurological diseases.					
34564432	9	41	theme	Man-Tf	1212:1217	arg1	levels					1194:1199	levels	1194:1199	levels of p-tau x Man-Tf	1194:1217	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	6	42	theme	Man-Tf	684:689	arg1	levels					691:696	Man-Tf levels	684:696	Man-Tf levels	684:696	Man-Tf levels were analyzed in CSF of patients with neurological diseases.					
34564432	5	43	theme	CSF	627:629	arg1	Man-Tf					631:636	CSF Man-Tf	627:636	CSF Man-Tf	627:636	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	9	44	theme	89	1341:1342	arg1	%					1343:1343	%	1343:1343	%	1343:1343	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
34564432	1	45	theme	type-specific	110:122	arg1	Glycosylation					86:98	Glycosylation	86:98	Glycosylation	86:98	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	1	45	theme	type-specific	110:122	arg1	modification					143:154	a cell type-specific post-translational modification	103:154	a cell type-specific post-translational modification that can be used for biomarker identification in various diseases	103:220	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	5	46	attach	derived	659:665	arg1	cortex					676:681	the cortex	672:681	the cortex	672:681	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	5	46	attach	derived	659:665	arg2	Man-Tf					631:636	CSF Man-Tf	627:636	CSF Man-Tf	627:636	The cerebral cortex contained Man-Tf as a major isofom, suggesting that CSF Man-Tf is, at least partly, derived from the cortex.					
34564432	1	47	theme	post-translational	124:141	arg1	Glycosylation					86:98	Glycosylation	86:98	Glycosylation	86:98	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	1	47	theme	post-translational	124:141	arg1	modification					143:154	a cell type-specific post-translational modification	103:154	a cell type-specific post-translational modification that can be used for biomarker identification in various diseases	103:220	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	7	48	theme	cognitive	828:836	arg1	impairment					838:847	mild cognitive impairment	823:847	mild cognitive impairment	823:847	Concentrations of Man-Tf were significantly increased in AD and mild cognitive impairment (MCI) comparing with other neurological diseases, and the levels correlated well with those of phosphorylated-tau (p-tau), a representative AD marker.					
34564432	3	49	theme	mass	449:452	arg1	spectrometry					454:465	mass spectrometry	449:465	mass spectrometry	449:465	Glycan structures of CSF Tf were analyzed by ultra-performance liquid chromatography followed by mass spectrometry.					
34564432	1	50	from	identification	187:200	arg1	diseases					213:220	various diseases	205:220	various diseases	205:220	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	8	51	theme	combined	1123:1130	arg1	p-tau					1132:1136	a combined p-tau	1121:1136	a combined p-tau	1121:1136	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	8	51	theme	combined	1123:1130	arg1	measure					1149:1155	Man-Tf measure	1142:1155	Man-Tf measure	1142:1155	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	8	51	theme	combined	1123:1130	arg1	biomarker					1168:1176	a biomarker	1166:1176	a biomarker for AD	1166:1183	Consistent with the observation, p-tau and Tf were co-expressed in hippocampal neurons of AD, leading to the notion that a combined p-tau and Man-Tf measure could be a biomarker for AD.					
34564432	1	52	used	used	168:171	arg2	Glycosylation					86:98	Glycosylation	86:98	Glycosylation	86:98	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	1	52	used	used	168:171	arg2	modification					143:154	a cell type-specific post-translational modification	103:154	a cell type-specific post-translational modification that can be used for biomarker identification in various diseases	103:220	Glycosylation is a cell type-specific post-translational modification that can be used for biomarker identification in various diseases.					
34564432	9	53	theme	84	1267:1268	arg1	%					1269:1269	%	1269:1269	%	1269:1269	Indeed, levels of p-tau x Man-Tf showed high diagnostic accuracy for MCI and AD; 84% sensitivities and 90% specificities for MCI and 94% sensitivities and 89% specificities for AD.					
31825043	0	0	theme	sodium-treated	87:100	arg1	mice					102:105	dextran sulfate sodium-treated mice	71:105	dextran sulfate sodium-treated mice	71:105	Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice.					
31825043	5	1	theme	Bifidobacterium	924:938	arg1	proportion					910:919	the proportion	906:919	the proportion of Bifidobacterium in colitic mice	906:954	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	0	2	theme	sulfate	79:85	arg1	mice					102:105	dextran sulfate sodium-treated mice	71:105	dextran sulfate sodium-treated mice	71:105	Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice.					
31825043	6	3	theme	cytokines	1131:1139	arg1	expression					1100:1109	the expression	1096:1109	the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice	1096:1232	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	1	4	theme	important	172:180	arg1	role					182:185	an important role	169:185	an important role	169:185	Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of colitis and that its composition could be modulated by exposure to dietary components.					
31825043	8	5	theme	mouse	1542:1546	arg1	model					1548:1552	a colitis mouse model	1532:1552	a colitis mouse model	1532:1552	Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.					
31825043	5	6	dep	genera	839:844	arg1	Sutterella					866:875	Sutterella	866:875	Sutterella	866:875	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	5	6	dep	genera	839:844	arg1	Dorea					859:863	Dorea	859:863	Dorea	859:863	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	6	7	from	expression	1100:1109	arg1	colon					1208:1212	the colon	1204:1212	the colon of DSS-treated mice	1204:1232	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	0	8	from	microbiota	57:66	arg1	mice					102:105	dextran sulfate sodium-treated mice	71:105	dextran sulfate sodium-treated mice	71:105	Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice.					
31825043	5	9	from	proportion	910:919	arg1	mice					951:954	colitic mice	943:954	colitic mice	943:954	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	TNF-α					1195:1199	TNF-α	1195:1199	TNF-α	1195:1199	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	IL-2					1149:1152	IL-2	1149:1152	IL-2	1149:1152	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	IFN-γ					1155:1159	IFN-γ	1155:1159	IFN-γ	1155:1159	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	IL-6					1177:1180	IL-6	1177:1180	IL-6	1177:1180	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	KC/GRO					1183:1188	KC/GRO	1183:1188	KC/GRO	1183:1188	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	cytokines					1131:1139	pro-inflammatory cytokines	1114:1139	pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α	1114:1199	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	GM-CSF					1162:1167	GM-CSF	1162:1167	GM-CSF	1162:1167	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	6	10	theme	pro-inflammatory	1114:1129	arg1	IL-1β					1170:1174	IL-1β	1170:1174	IL-1β	1170:1174	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	3	11	theme	gut	482:484	arg1	composition					497:507	gut microbiota composition	482:507	gut microbiota composition	482:507	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	4	12	theme	community	729:737	arg1	diversity					739:747	microbial community diversity	719:747	microbial community diversity in DSS-treated mice	719:767	Our results supported our hypothesis that dietary resveratrol altered the microbial composition and restored microbial community diversity in DSS-treated mice.					
31825043	7	13	theme	relative	1313:1320	arg1	levels					1322:1327	the relative levels	1309:1327	the relative levels of these pro-inflammatory cytokines	1309:1363	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	7	14	theme	cytokines	1355:1363	arg1	alterations					1369:1379	alterations	1369:1379	alterations of the gut microbiota	1369:1401	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	7	14	theme	cytokines	1355:1363	arg1	levels					1322:1327	the relative levels	1309:1327	the relative levels of these pro-inflammatory cytokines	1309:1363	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	2	15	theme	components	394:403	arg1	administration					368:381	administration	368:381	administration of dietary components	368:403	Thus, it may be possible to ameliorate the severity of colitis through administration of dietary components.					
31825043	3	16	dep	composition	497:507	arg1	the					478:480	the	478:480	the	478:480	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	2	17	theme	dietary	386:392	arg1	components					394:403	dietary components	386:403	dietary components	386:403	Thus, it may be possible to ameliorate the severity of colitis through administration of dietary components.					
31825043	5	18	theme	genera	839:844	arg1	abundance					822:830	the abundance	818:830	the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila	818:889	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	3	19	theme	inflammatory	527:538	arg1	status					540:545	the resulting inflammatory status	513:545	the resulting inflammatory status	513:545	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	6	20	theme	weight	1005:1010	arg1	loss					1012:1015	mouse body weight loss	994:1015	mouse body weight loss	994:1015	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	0	21	theme	Dietary	0:6	arg1	resveratrol					8:18	Dietary resveratrol	0:18	Dietary resveratrol	0:18	Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice.					
31825043	1	22	theme	dietary	277:283	arg1	components					285:294	dietary components	277:294	dietary components	277:294	Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of colitis and that its composition could be modulated by exposure to dietary components.					
31825043	6	23	theme	mice	1229:1232	arg1	colon					1208:1212	the colon	1204:1212	the colon of DSS-treated mice	1204:1232	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	4	24	theme	DSS-treated	752:762	arg1	mice					764:767	DSS-treated mice	752:767	DSS-treated mice	752:767	Our results supported our hypothesis that dietary resveratrol altered the microbial composition and restored microbial community diversity in DSS-treated mice.					
31825043	6	25	theme	body	1000:1003	arg1	loss					1012:1015	mouse body weight loss	994:1015	mouse body weight loss	994:1015	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	7	26	theme	gut	1388:1390	arg1	microbiota					1392:1401	the gut microbiota	1384:1401	the gut microbiota	1384:1401	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	1	27	theme	Accumulating	108:119	arg1	evidence					121:128	Accumulating evidence	108:128	Accumulating evidence	108:128	Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of colitis and that its composition could be modulated by exposure to dietary components.					
31825043	6	28	theme	DSS-treated	1217:1227	arg1	mice					1229:1232	DSS-treated mice	1217:1232	DSS-treated mice	1217:1232	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	3	29	theme	resveratrol	463:473	arg1	effects					432:438	the effects	428:438	the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model	428:607	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	6	30	theme	mouse	994:998	arg1	loss					1012:1015	mouse body weight loss	994:1015	mouse body weight loss	994:1015	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	1	31	theme	colitis	210:216	arg1	pathogenesis					194:205	the pathogenesis	190:205	the pathogenesis of colitis	190:216	Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of colitis and that its composition could be modulated by exposure to dietary components.					
31825043	7	32	theme	significant	1276:1286	arg1	correlations					1288:1299	significant correlations	1276:1299	significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota	1276:1401	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	8	33	theme	inflammatory	1469:1480	arg1	status					1482:1487	the inflammatory status	1465:1487	the inflammatory status	1465:1487	Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.					
31825043	3	34	theme	colitis	589:595	arg1	model					603:607	a dextran sodium sulfate (DSS)-induced colitis mouse model	550:607	a dextran sodium sulfate (DSS)-induced colitis mouse model	550:607	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	6	35	theme	tissue	1063:1068	arg1	damage					1070:1075	tissue damage	1063:1075	tissue damage	1063:1075	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	3	36	theme	mouse	597:601	arg1	model					603:607	a dextran sodium sulfate (DSS)-induced colitis mouse model	550:607	a dextran sodium sulfate (DSS)-induced colitis mouse model	550:607	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	3	37	theme	administered	450:461	arg1	resveratrol					463:473	orally administered resveratrol	443:473	orally administered resveratrol	443:473	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	8	38	theme	microbiota	1508:1517	arg1	dysbiosis					1519:1527	gut microbiota dysbiosis	1504:1527	gut microbiota dysbiosis	1504:1527	Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.					
31825043	3	39	theme	microbiota	486:495	arg1	composition					497:507	gut microbiota composition	482:507	gut microbiota composition	482:507	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	0	40	theme	gut	53:55	arg1	microbiota					57:66	gut microbiota	53:66	gut microbiota in dextran sulfate sodium-treated mice	53:105	Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice.					
31825043	7	41	theme	microbiota	1392:1401	arg1	alterations					1369:1379	alterations	1369:1379	alterations of the gut microbiota	1369:1401	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	7	41	theme	microbiota	1392:1401	arg1	levels					1322:1327	the relative levels	1309:1327	the relative levels of these pro-inflammatory cytokines	1309:1363	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	3	42	theme	-induced	580:587	arg1	model					603:607	a dextran sodium sulfate (DSS)-induced colitis mouse model	550:607	a dextran sodium sulfate (DSS)-induced colitis mouse model	550:607	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	7	43	theme	pro-inflammatory	1338:1353	arg1	cytokines					1355:1363	these pro-inflammatory cytokines	1332:1363	these pro-inflammatory cytokines	1332:1363	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	4	44	theme	microbial	719:727	arg1	diversity					739:747	microbial community diversity	719:747	microbial community diversity in DSS-treated mice	719:767	Our results supported our hypothesis that dietary resveratrol altered the microbial composition and restored microbial community diversity in DSS-treated mice.					
31825043	8	45	theme	gut	1504:1506	arg1	dysbiosis					1519:1527	gut microbiota dysbiosis	1504:1527	gut microbiota dysbiosis	1504:1527	Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.					
31825043	4	46	theme	microbial	684:692	arg1	composition					694:704	the microbial composition	680:704	the microbial composition	680:704	Our results supported our hypothesis that dietary resveratrol altered the microbial composition and restored microbial community diversity in DSS-treated mice.					
31825043	7	47	theme	correlation	1245:1255	arg1	analysis					1257:1264	Pearson's correlation analysis	1235:1264	Pearson's correlation analysis	1235:1264	Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota.					
31825043	4	48	from	diversity	739:747	arg1	mice					764:767	DSS-treated mice	752:767	DSS-treated mice	752:767	Our results supported our hypothesis that dietary resveratrol altered the microbial composition and restored microbial community diversity in DSS-treated mice.					
31825043	5	49	theme	colitic	943:949	arg1	mice					951:954	colitic mice	943:954	colitic mice	943:954	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	8	50	theme	dietary	1434:1440	arg1	resveratrol					1442:1452	dietary resveratrol	1434:1452	dietary resveratrol	1434:1452	Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.					
31825043	1	51	theme	gut	148:150	arg1	microbiota					152:161	the gut microbiota	144:161	the gut microbiota	144:161	Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of colitis and that its composition could be modulated by exposure to dietary components.					
31825043	0	52	theme	dextran	71:77	arg1	sulfate					79:85	dextran sulfate	71:85	dextran sulfate sodium-treated mice	71:105	Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice.					
31825043	5	53	theme	Bilophila	881:889	arg1	abundance					822:830	the abundance	818:830	the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila	818:889	Specifically, resveratrol effectively decreased the abundance of the genera Akkermansia, Dorea, Sutterella and Bilophila, and increased the proportion of Bifidobacterium in colitic mice.					
31825043	3	54	theme	model	603:607	arg1	status					540:545	the resulting inflammatory status	513:545	the resulting inflammatory status	513:545	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	3	54	theme	model	603:607	arg1	composition					497:507	gut microbiota composition	482:507	gut microbiota composition	482:507	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	3	55	theme	resulting	517:525	arg1	status					540:545	the resulting inflammatory status	513:545	the resulting inflammatory status	513:545	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	4	56	theme	dietary	652:658	arg1	resveratrol					660:670	dietary resveratrol	652:670	dietary resveratrol	652:670	Our results supported our hypothesis that dietary resveratrol altered the microbial composition and restored microbial community diversity in DSS-treated mice.					
31825043	6	57	theme	activity	1037:1044	arg1	index					1046:1050	the disease activity index	1025:1050	the disease activity index	1025:1050	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
31825043	8	58	theme	colitis	1534:1540	arg1	model					1548:1552	a colitis mouse model	1532:1552	a colitis mouse model	1532:1552	Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.					
31825043	3	59	from	effects	432:438	arg1	status					540:545	the resulting inflammatory status	513:545	the resulting inflammatory status	513:545	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	3	59	from	effects	432:438	arg1	composition					497:507	gut microbiota composition	482:507	gut microbiota composition	482:507	Herein, we determined the effects of orally administered resveratrol on the gut microbiota composition and the resulting inflammatory status of a dextran sodium sulfate (DSS)-induced colitis mouse model.					
31825043	2	60	theme	colitis	352:358	arg1	severity					340:347	the severity	336:347	the severity of colitis	336:358	Thus, it may be possible to ameliorate the severity of colitis through administration of dietary components.					
31825043	6	61	theme	disease	1029:1035	arg1	index					1046:1050	the disease activity index	1025:1050	the disease activity index	1025:1050	Resveratrol was also able to prevent mouse body weight loss, reduce the disease activity index, attenuate tissue damage, and down-regulate the expression of pro-inflammatory cytokines such as IL-2, IFN-γ, GM-CSF, IL-1β, IL-6, KC/GRO, and TNF-α in the colon of DSS-treated mice.					
33422802	7	0	theme	total	1090:1094	arg1	DVT					1096:1098	total DVT	1090:1098	total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01)	1090:1136	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	8	1	from	difference	1227:1236	arg1	RR					1557:1558	RR	1557:1558	RR	1557:1558	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	1	from	difference	1227:1236	arg1	RR					1489:1490	RR	1489:1490	RR	1489:1490	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	1	from	difference	1227:1236	arg1	mortality					1546:1554	bleeding-related mortality	1529:1554	bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77)	1529:1591	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	1	from	difference	1227:1236	arg1	mortality					1418:1426	all-cause mortality	1408:1426	all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83)	1408:1463	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	1	from	difference	1227:1236	arg1	mortality					1478:1486	VTE-related mortality	1466:1486	VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64)	1466:1523	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	1	from	difference	1227:1236	arg1	RR					1429:1430	RR	1429:1430	RR	1429:1430	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	1	from	difference	1227:1236	arg1	incidence					1265:1273	the incidence	1261:1273	the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52)	1261:1405	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	7	2	dep	DVT	1096:1098	arg1	RR					1101:1102	RR	1101:1102	RR	1101:1102	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	5	3	theme	Cochrane	512:519	arg1	Collaboration					521:533	the Cochrane Collaboration	508:533	the Cochrane Collaboration Central Register	508:550	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	9	4	dep	associated	1682:1691	arg1	compared					1744:1751	compared	1744:1751	compared to LMWH	1744:1759	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	6	5	with	criteria	757:764	arg1	age					806:808	a mean age	799:808	a mean age of 68 years	799:820	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	8	6	dep	RR	1371:1372	arg1	P=0.52					1399:1404	P=0.52	1399:1404	P=0.52	1399:1404	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	6	dep	RR	1371:1372	arg1	%					1382:1382	95% CI: 0.92-1.17	1380:1396	RR 1.04; 95% CI: 0.92-1.17; P=0.52	1371:1404	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	6	7	theme	68 years	813:820	arg1	age					806:808	a mean age	799:808	a mean age of 68 years	799:820	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	7	8	theme	mortality	1038:1046	arg1	events					950:955	the composite events	936:955	the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42)	936:1185	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	4	9	dep	efficacy	380:387	arg1	the					376:378	the	376:378	the	376:378	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	8	10	dep	bleeding	1284:1291	arg1	relevant					1342:1349	relevant	1342:1349	relevant	1342:1349	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	10	dep	bleeding	1284:1291	arg1	RR					1294:1295	RR 0.99	1294:1300	RR 0.99; 95% CI: 0.77-1.27; P=0.92	1294:1327	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	7	11	dep	%	1113:1113	arg1	P<0.01					1130:1135	P<0.01	1130:1135	0.59; 95% CI: 0.48-0.73; P<0.01	1105:1135	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	8	12	theme	VTE-related	1466:1476	arg1	RR					1489:1490	RR	1489:1490	RR	1489:1490	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	12	theme	VTE-related	1466:1476	arg1	mortality					1478:1486	VTE-related mortality	1466:1486	VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64)	1466:1523	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	7	13	theme	VTE-related	1026:1036	arg1	mortality					1038:1046	VTE-related mortality	1026:1046	VTE-related mortality	1026:1046	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	6	14	theme	mean	801:804	arg1	age					806:808	a mean age	799:808	a mean age of 68 years	799:820	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	4	15	from	safety	393:398	arg1	surgery					458:464	orthopedic surgery	447:464	orthopedic surgery	447:464	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	7	16	theme	composite	940:948	arg1	events					950:955	the composite events	936:955	the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42)	936:1185	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	2	17	theme	Low	158:160	arg1	heparin					179:185	Low molecular weight heparin	158:185	Low molecular weight heparin (LMWH)	158:192	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	17	theme	Low	158:160	arg1	standard					207:214	standard	207:214	standard	207:214	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	17	theme	Low	158:160	arg1	LMWH					188:191	LMWH	188:191	LMWH	188:191	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	7	18	theme	vein	974:977	arg1	DVT					991:993	DVT	991:993	DVT	991:993	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	18	theme	vein	974:977	arg1	thrombosis					979:988	proximal deep vein thrombosis	960:988	proximal deep vein thrombosis (DVT)	960:994	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	18	theme	vein	974:977	arg1	RR					1049:1050	RR	1049:1050	RR	1049:1050	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	19	theme	major	914:918	arg1	VTE					920:922	major VTE	914:922	major VTE	914:922	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	1	20	theme	BACKGROUND	69:78	arg1	complication					122:133	a serious complication	112:133	a serious complication of orthopedic surgery	112:155	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	1	20	theme	BACKGROUND	69:78	arg1	VTE					104:106	VTE	104:106	VTE	104:106	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	1	20	theme	BACKGROUND	69:78	arg1	thromboembolism					87:101	BACKGROUND Venous thromboembolism	69:101	BACKGROUND Venous thromboembolism (VTE)	69:107	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	2	21	from	standard	207:214	arg1	population					255:264	this population	250:264	this population	250:264	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	8	22	theme	all-cause	1408:1416	arg1	mortality					1418:1426	all-cause mortality	1408:1426	all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83)	1408:1463	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	22	theme	all-cause	1408:1416	arg1	RR					1429:1430	RR	1429:1430	RR	1429:1430	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	3	23	used	used	334:337	arg2	alternatives					342:353	alternatives	342:353	alternatives	342:353	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	3	23	used	used	334:337	arg2	DOACs					304:308	DOACs	304:308	DOACs	304:308	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	3	23	used	used	334:337	arg2	anticoagulants					288:301	direct oral anticoagulants	276:301	direct oral anticoagulants (DOACs)	276:309	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	7	24	theme	VTE	920:922	arg1	reduction					901:909	a significant reduction	887:909	a significant reduction of major VTE	887:922	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	1	25	theme	Venous	80:85	arg1	complication					122:133	a serious complication	112:133	a serious complication of orthopedic surgery	112:155	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	1	25	theme	Venous	80:85	arg1	VTE					104:106	VTE	104:106	VTE	104:106	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	1	25	theme	Venous	80:85	arg1	thromboembolism					87:101	BACKGROUND Venous thromboembolism	69:101	BACKGROUND Venous thromboembolism (VTE)	69:107	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	7	26	theme	PE	1147:1148	arg1	events					950:955	the composite events	936:955	the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42)	936:1185	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	5	27	theme	randomized	610:619	arg1	RCTs					640:643	RCTs	640:643	RCTs	640:643	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	27	theme	randomized	610:619	arg1	trials					632:637	randomized controlled trials	610:637	randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery	610:715	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	28	theme	Controlled	555:564	arg1	Trials					566:571	Controlled Trials	555:571	Controlled Trials	555:571	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	7	29	theme	thrombosis	979:988	arg1	events					950:955	the composite events	936:955	the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42)	936:1185	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	30	theme	DVT	1096:1098	arg1	events					950:955	the composite events	936:955	the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42)	936:1185	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	5	31	theme	controlled	621:630	arg1	RCTs					640:643	RCTs	640:643	RCTs	640:643	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	31	theme	controlled	621:630	arg1	trials					632:637	randomized controlled trials	610:637	randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery	610:715	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	32	theme	Trials	566:571	arg1	MEDLINE					487:493	MEDLINE	487:493	MEDLINE	487:493	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	32	theme	Trials	566:571	arg1	Register					543:550	the Cochrane Collaboration Central Register	508:550	the Cochrane Collaboration Central Register	508:550	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	32	theme	Trials	566:571	arg1	Embase					496:501	Embase	496:501	Embase	496:501	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	9	33	theme	DVT	1739:1741	arg1	reduction					1712:1720	a significant reduction	1698:1720	a significant reduction of major VTE and DVT	1698:1741	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	9	34	theme	major	1725:1729	arg1	VTE					1731:1733	major VTE	1725:1733	major VTE	1725:1733	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	9	35	theme	significant	1700:1710	arg1	reduction					1712:1720	a significant reduction	1698:1720	a significant reduction of major VTE and DVT	1698:1741	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	7	36	dep	RR	1101:1102	arg1	%					1113:1113	0.59; 95% CI: 0.48-0.73	1105:1127	0.59; 95% CI: 0.48-0.73; P<0.01	1105:1135	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	8	37	theme	%	1305:1305	arg1	CI					1307:1308	95% CI	1303:1308	RR 0.99; 95% CI: 0.77-1.27; P=0.92	1294:1327	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	7	38	theme	embolism	1007:1014	arg1	events					950:955	the composite events	936:955	the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42)	936:1185	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	39	theme	significant	889:899	arg1	reduction					901:909	a significant reduction	887:909	a significant reduction of major VTE	887:922	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	9	40	theme	orthopedic	1629:1638	arg1	surgery					1640:1646	orthopedic surgery	1629:1646	orthopedic surgery	1629:1646	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	2	41	theme	care	219:222	arg1	heparin					179:185	Low molecular weight heparin	158:185	Low molecular weight heparin (LMWH)	158:192	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	41	theme	care	219:222	arg1	standard					207:214	standard	207:214	standard	207:214	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	7	42	dep	RR	1151:1152	arg1	P=0.42					1179:1184	P=0.42	1179:1184	P=0.42	1179:1184	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	42	dep	RR	1151:1152	arg1	%					1162:1162	95% CI: 0.49-1.34	1160:1176	RR 0.81; 95% CI: 0.49-1.34; P=0.42	1151:1184	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	0	43	theme	orthopedic	23:32	arg1	surgery					34:40	orthopedic surgery	23:40	orthopedic surgery	23:40	Thromboprophylaxis for orthopedic surgery; An updated meta-analysis.					
33422802	4	44	from	efficacy	380:387	arg1	surgery					458:464	orthopedic surgery	447:464	orthopedic surgery	447:464	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	8	45	dep	RR	1489:1490	arg1	P=0.64					1517:1522	P=0.64	1517:1522	P=0.64	1517:1522	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	45	dep	RR	1489:1490	arg1	%					1500:1500	95% CI: 0.40-1.74	1498:1514	RR 0.84; 95% CI: 0.40-1.74; P=0.64	1489:1522	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	5	46	from	inception	578:586	arg1	MEDLINE					487:493	MEDLINE	487:493	MEDLINE	487:493	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	46	from	inception	578:586	arg1	Register					543:550	the Cochrane Collaboration Central Register	508:550	the Cochrane Collaboration Central Register	508:550	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	46	from	inception	578:586	arg1	Embase					496:501	Embase	496:501	Embase	496:501	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	5	47	theme	orthopedic	698:707	arg1	surgery					709:715	orthopedic surgery	698:715	orthopedic surgery	698:715	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	8	48	theme	significant	1215:1225	arg1	difference					1227:1236	no statistically significant difference	1198:1236	no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77)	1198:1591	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	0	49	dep	meta-analysis	54:66	arg1	Thromboprophylaxis					0:17	Thromboprophylaxis	0:17	Thromboprophylaxis for orthopedic surgery	0:40	Thromboprophylaxis for orthopedic surgery; An updated meta-analysis.					
33422802	8	50	dep	RR	1557:1558	arg1	P=0.77					1585:1590	P=0.77	1585:1590	P=0.77	1585:1590	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	50	dep	RR	1557:1558	arg1	%					1568:1568	95% CI: 0.30-5.18	1566:1582	RR 1.24; 95% CI: 0.30-5.18; P=0.77	1557:1590	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	7	51	theme	pulmonary	997:1005	arg1	PE					1017:1018	PE	1017:1018	PE	1017:1018	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	51	theme	pulmonary	997:1005	arg1	embolism					1007:1014	pulmonary embolism	997:1014	pulmonary embolism (PE)	997:1019	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	4	52	theme	orthopedic	447:456	arg1	surgery					458:464	orthopedic surgery	447:464	orthopedic surgery	447:464	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	8	53	theme	bleeding-related	1529:1544	arg1	RR					1557:1558	RR	1557:1558	RR	1557:1558	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	53	theme	bleeding-related	1529:1544	arg1	mortality					1546:1554	bleeding-related mortality	1529:1554	bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77)	1529:1591	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	5	54	theme	Collaboration	521:533	arg1	Register					543:550	the Cochrane Collaboration Central Register	508:550	the Cochrane Collaboration Central Register	508:550	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	9	55	dep	CONCLUSION	1594:1603	arg1	patients					1609:1616	patients	1609:1616	patients undergoing orthopedic surgery	1609:1646	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	2	56	theme	molecular	162:170	arg1	heparin					179:185	Low molecular weight heparin	158:185	Low molecular weight heparin (LMWH)	158:192	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	56	theme	molecular	162:170	arg1	standard					207:214	standard	207:214	standard	207:214	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	56	theme	molecular	162:170	arg1	LMWH					188:191	LMWH	188:191	LMWH	188:191	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	5	57	theme	Central	535:541	arg1	Register					543:550	the Cochrane Collaboration Central Register	508:550	the Cochrane Collaboration Central Register	508:550	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	0	58	theme	updated	46:52	arg1	meta-analysis					54:66	An updated meta-analysis	43:66	An updated meta-analysis	43:66	Thromboprophylaxis for orthopedic surgery; An updated meta-analysis.					
33422802	4	59	theme	DOACs	403:407	arg1	efficacy					380:387	efficacy	380:387	efficacy	380:387	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	4	59	theme	DOACs	403:407	arg1	safety					393:398	safety	393:398	safety	393:398	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	6	60	theme	inclusion	747:755	arg1	patients					784:791	40,438 patients	777:791	40,438 patients	777:791	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	6	60	theme	inclusion	747:755	arg1	criteria					757:764	met inclusion criteria	743:764	met inclusion criteria	743:764	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	1	61	theme	serious	114:120	arg1	complication					122:133	a serious complication	112:133	a serious complication of orthopedic surgery	112:155	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	1	61	theme	serious	114:120	arg1	thromboembolism					87:101	BACKGROUND Venous thromboembolism	69:101	BACKGROUND Venous thromboembolism (VTE)	69:107	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	8	62	dep	RR	1429:1430	arg1	P=0.83					1457:1462	P=0.83	1457:1462	P=0.83	1457:1462	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	62	dep	RR	1429:1430	arg1	%					1440:1440	95% CI: 0.64-1.76	1438:1454	RR 1.06; 95% CI: 0.64-1.76; P=0.83	1429:1462	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	3	63	theme	direct	276:281	arg1	DOACs					304:308	DOACs	304:308	DOACs	304:308	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	3	63	theme	direct	276:281	arg1	anticoagulants					288:301	direct oral anticoagulants	276:301	direct oral anticoagulants (DOACs)	276:309	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	3	63	theme	direct	276:281	arg1	alternatives					342:353	alternatives	342:353	alternatives	342:353	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	10	64	theme	Safety	1762:1767	arg1	outcomes					1769:1776	Safety outcomes	1762:1776	Safety outcomes	1762:1776	Safety outcomes were not significantly different between both treatment groups.					
33422802	6	65	theme	met	743:745	arg1	patients					784:791	40,438 patients	777:791	40,438 patients	777:791	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	6	65	theme	met	743:745	arg1	criteria					757:764	met inclusion criteria	743:764	met inclusion criteria	743:764	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	2	66	from	population	255:264	arg1	heparin					179:185	Low molecular weight heparin	158:185	Low molecular weight heparin (LMWH)	158:192	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	66	from	population	255:264	arg1	standard					207:214	standard	207:214	standard	207:214	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	5	67	from	LMWH	667:670	arg1	surgery					709:715	orthopedic surgery	698:715	orthopedic surgery	698:715	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	3	68	theme	oral	283:286	arg1	DOACs					304:308	DOACs	304:308	DOACs	304:308	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	3	68	theme	oral	283:286	arg1	anticoagulants					288:301	direct oral anticoagulants	276:301	direct oral anticoagulants (DOACs)	276:309	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	3	68	theme	oral	283:286	arg1	alternatives					342:353	alternatives	342:353	alternatives	342:353	However, direct oral anticoagulants (DOACs) are increasingly being used as alternatives.					
33422802	4	69	theme	LMWH	416:419	arg1	efficacy					380:387	efficacy	380:387	efficacy	380:387	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	4	69	theme	LMWH	416:419	arg1	safety					393:398	safety	393:398	safety	393:398	OBJECTIVE To assess the efficacy and safety of DOACs versus LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	7	70	theme	deep	969:972	arg1	DVT					991:993	DVT	991:993	DVT	991:993	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	70	theme	deep	969:972	arg1	thrombosis					979:988	proximal deep vein thrombosis	960:988	proximal deep vein thrombosis (DVT)	960:994	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	70	theme	deep	969:972	arg1	RR					1049:1050	RR	1049:1050	RR	1049:1050	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	5	71	dep	METHODS	467:473	arg1	searched					478:485	searched	478:485	searched	478:485	METHODS We searched MEDLINE, Embase, and the Cochrane Collaboration Central Register of Controlled Trials from inception until April 2020, for randomized controlled trials (RCTs) comparing DOACs with LMWH for thromboprophylaxis in orthopedic surgery.					
33422802	6	72	dep	RCTs	738:741	arg1	patients					784:791	40,438 patients	777:791	40,438 patients	777:791	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	6	72	dep	RCTs	738:741	arg1	criteria					757:764	met inclusion criteria	743:764	met inclusion criteria	743:764	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	10	73	theme	treatment	1824:1832	arg1	groups					1834:1839	both treatment groups	1819:1839	both treatment groups	1819:1839	Safety outcomes were not significantly different between both treatment groups.					
33422802	9	74	with	thromboprophylaxis	1649:1666	arg1	DOACs					1673:1677	DOACs	1673:1677	DOACs	1673:1677	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	7	75	theme	proximal	960:967	arg1	DVT					991:993	DVT	991:993	DVT	991:993	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	75	theme	proximal	960:967	arg1	thrombosis					979:988	proximal deep vein thrombosis	960:988	proximal deep vein thrombosis (DVT)	960:994	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	75	theme	proximal	960:967	arg1	RR					1049:1050	RR	1049:1050	RR	1049:1050	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	2	76	theme	weight	172:177	arg1	heparin					179:185	Low molecular weight heparin	158:185	Low molecular weight heparin (LMWH)	158:192	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	76	theme	weight	172:177	arg1	standard					207:214	standard	207:214	standard	207:214	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	2	76	theme	weight	172:177	arg1	LMWH					188:191	LMWH	188:191	LMWH	188:191	Low molecular weight heparin (LMWH) has been the standard of care for thromboprophylaxis in this population.					
33422802	6	77	theme	Twenty-five	726:736	arg1	RCTs					738:741	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50%	718:828	RCTs	738:741	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	1	78	theme	orthopedic	138:147	arg1	surgery					149:155	orthopedic surgery	138:155	orthopedic surgery	138:155	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	8	79	theme	bleeding	1361:1368	arg1	RR					1557:1558	RR	1557:1558	RR	1557:1558	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	79	theme	bleeding	1361:1368	arg1	RR					1489:1490	RR	1489:1490	RR	1489:1490	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	79	theme	bleeding	1361:1368	arg1	mortality					1546:1554	bleeding-related mortality	1529:1554	bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77)	1529:1591	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	79	theme	bleeding	1361:1368	arg1	mortality					1418:1426	all-cause mortality	1408:1426	all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83)	1408:1463	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	79	theme	bleeding	1361:1368	arg1	mortality					1478:1486	VTE-related mortality	1466:1486	VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64)	1466:1523	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	79	theme	bleeding	1361:1368	arg1	RR					1429:1430	RR	1429:1430	RR	1429:1430	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	79	theme	bleeding	1361:1368	arg1	incidence					1265:1273	the incidence	1261:1273	the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52)	1261:1405	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	9	80	theme	VTE	1731:1733	arg1	reduction					1712:1720	a significant reduction	1698:1720	a significant reduction of major VTE and DVT	1698:1741	CONCLUSION For patients undergoing orthopedic surgery, thromboprophylaxis with DOACs is associated with a significant reduction of major VTE and DVT, compared to LMWH.					
33422802	7	81	dep	RR	1049:1050	arg1	P<0.01					1077:1082	P<0.01	1077:1082	RR 0.33; 95% CI: 0.20-0.53; P<0.01	1049:1082	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	7	81	dep	RR	1049:1050	arg1	%					1060:1060	95% CI: 0.20-0.53	1058:1074	RR 0.33; 95% CI: 0.20-0.53; P<0.01	1049:1082	Compared to LMWH, DOACs were associated with a significant reduction of major VTE; defined as the composite events of proximal deep vein thrombosis (DVT), pulmonary embolism (PE), and VTE-related mortality (RR 0.33; 95% CI: 0.20-0.53; P<0.01), and total DVT (RR: 0.59; 95% CI: 0.48-0.73; P<0.01), but not PE (RR 0.81; 95% CI: 0.49-1.34; P=0.42).					
33422802	6	82	theme	RESULTS	718:724	arg1	RCTs					738:741	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50%	718:828	RCTs	738:741	RESULTS Twenty-five RCTs met inclusion criteria, including 40,438 patients, with a mean age of 68 years and 50% were males.					
33422802	1	83	theme	surgery	149:155	arg1	complication					122:133	a serious complication	112:133	a serious complication of orthopedic surgery	112:155	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	1	83	theme	surgery	149:155	arg1	thromboembolism					87:101	BACKGROUND Venous thromboembolism	69:101	BACKGROUND Venous thromboembolism (VTE)	69:107	BACKGROUND Venous thromboembolism (VTE) is a serious complication of orthopedic surgery.					
33422802	8	84	dep	RR	1294:1295	arg1	P=0.92					1322:1327	P=0.92	1322:1327	P=0.92	1322:1327	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	84	dep	RR	1294:1295	arg1	0.77-1.27					1311:1319	0.77-1.27	1311:1319	0.77-1.27	1311:1319	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33422802	8	84	dep	RR	1294:1295	arg1	CI					1307:1308	95% CI	1303:1308	RR 0.99; 95% CI: 0.77-1.27; P=0.92	1294:1327	There was no statistically significant difference between both groups on the incidence of major bleeding (RR 0.99; 95% CI: 0.77-1.27; P=0.92), clinically relevant non-major bleeding (RR 1.04; 95% CI: 0.92-1.17; P=0.52), all-cause mortality (RR 1.06; 95% CI: 0.64-1.76; P=0.83), VTE-related mortality (RR 0.84; 95% CI: 0.40-1.74; P=0.64) and bleeding-related mortality (RR 1.24; 95% CI: 0.30-5.18; P=0.77).					
33483019	0	0	theme	conductive	108:117	arg1	membranes					119:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes	53:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells	53:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	2	1	theme	QCS/PVA	604:610	arg1	matrix					618:623	QCS/PVA blend matrix	604:623	QCS/PVA blend matrix	604:623	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	3	2	theme	LDH	717:719	arg1	nanosheets					721:730	LDH nanosheets	717:730	LDH nanosheets	717:730	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	5	3	theme	ionic	1085:1089	arg1	conductivity					1091:1102	residual ionic conductivity	1076:1102	residual ionic conductivity of as high as 92 % after immersion	1076:1137	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	3	4	theme	LDH	822:824	arg1	nanosheets					826:835	LDH nanosheets	822:835	LDH nanosheets	822:835	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	0	5	theme	anion	102:106	arg1	membranes					119:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes	53:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells	53:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	5	6	theme	composite	1051:1059	arg1	membranes					1061:1069	the composite membranes	1047:1069	the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content	1047:1276	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	7	theme	ionic	182:186	arg1	conductivity					188:199	ionic conductivity	182:199	ionic conductivity	182:199	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	3	8	theme	nanosheets	826:835	arg1	alkali					785:790	alkali	785:790	alkali	785:790	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	8	theme	nanosheets	826:835	arg1	conductivity					771:782	impressive ion conductivity	756:782	impressive ion conductivity	756:782	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	8	theme	nanosheets	826:835	arg1	ability					811:817	fuel resistant ability	796:817	fuel resistant ability	796:817	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	1	9	theme	polyvinyl	344:352	arg1	PVA					363:365	PVA	363:365	PVA	363:365	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	9	theme	polyvinyl	344:352	arg1	alcohol					354:360	polyvinyl alcohol	344:360	polyvinyl alcohol (PVA)	344:366	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	5	10	theme	peak	1290:1293	arg1	density					1301:1307	a peak power density	1288:1307	a peak power density of 73 mW cm-2	1288:1321	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	11	theme	quaternized	272:282	arg1	membranes					254:262	anion exchange membranes	239:262	anion exchange membranes (AEMs)	239:269	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	11	theme	quaternized	272:282	arg1	QCS					294:296	QCS	294:296	QCS	294:296	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	11	theme	quaternized	272:282	arg1	chitosan					284:291	quaternized chitosan	272:291	quaternized chitosan (QCS)	272:297	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	4	12	theme	cm-1	938:941	arg1	higher					961:966	higher	961:966	higher	961:966	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	4	12	theme	cm-1	938:941	arg1	conductivity					914:925	the maximum OH- conductivity	898:925	the maximum OH- conductivity	898:925	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	3	13	theme	nanosheets	721:730	arg1	sites					708:712	the active sites	697:712	the active sites of LDH nanosheets	697:730	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	13	theme	nanosheets	721:730	arg1	nanosheets					721:730	LDH nanosheets	717:730	LDH nanosheets	717:730	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	4	14	theme	composite	869:877	arg1	membrane					879:886	The composite membrane	865:886	The composite membrane	865:886	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	5	15	theme	LDHs	1265:1268	arg1	content					1270:1276	6% LDHs content	1262:1276	6% LDHs content	1262:1276	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	2	16	dep	three-dimensional	419:435	arg1	3D					438:439	3D	438:439	3D	438:439	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	17	theme	composite	638:646	arg1	AEMs					648:651	composite AEMs	638:651	composite AEMs	638:651	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	0	18	theme	fuel	133:136	arg1	cells					138:142	fuel cells	133:142	fuel cells	133:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	3	19	theme	resistant	801:809	arg1	ability					811:817	fuel resistant ability	796:817	fuel resistant ability	796:817	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	4	20	theme	mS	935:936	arg1	cm-1					938:941	25.7 mS cm-1	930:941	25.7 mS cm-1	930:941	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	0	21	theme	nanoflower-like	3:17	arg1	hydroxide					34:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	2	22	theme	double	475:480	arg1	LDHs					494:497	LDHs	494:497	LDHs	494:497	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	22	theme	double	475:480	arg1	hydroxides					482:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides	419:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs)	419:498	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	5	23	theme	power	1295:1299	arg1	density					1301:1307	a peak power density	1288:1307	a peak power density of 73 mW cm-2	1288:1321	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	24	theme	mechanical	202:211	arg1	stability					226:234	mechanical and chemical stability	202:234	mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS)	202:297	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	0	25	theme	3D	0:1	arg1	hydroxide					34:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	5	26	theme	2	1144:1144	arg1	M					1146:1146	M	1146:1146	M	1146:1146	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	2	27	theme	blend	612:616	arg1	matrix					618:623	QCS/PVA blend matrix	604:623	QCS/PVA blend matrix	604:623	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	4	28	theme	pristine	985:992	arg1	membrane					994:1001	the pristine membrane	981:1001	the pristine membrane	981:1001	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	5	29	theme	mW	1384:1385	arg1	cm-2					1387:1390	only 40 mW cm-2	1376:1390	only 40 mW cm-2	1376:1390	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	30	theme	6	1262:1262	arg1	%					1263:1263	%	1263:1263	%	1263:1263	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	0	31	theme	double	27:32	arg1	hydroxide					34:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	5	32	theme	M	1146:1146	arg1	KOH					1148:1150	a 2 M KOH	1142:1150	a 2 M KOH for 100 h. Due to the decreased methanol permeability	1142:1204	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	2	33	theme	solvothermal	551:562	arg1	method					564:569	one-pot ethylene glycol-assisted solvothermal method	518:569	one-pot ethylene glycol-assisted solvothermal method	518:569	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	5	34	theme	pristine	1345:1352	arg1	membrane					1354:1361	the pristine membrane	1341:1361	the pristine membrane	1341:1361	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	35	theme	%	1263:1263	arg1	content					1270:1276	6% LDHs content	1262:1276	6% LDHs content	1262:1276	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	36	theme	chemical	217:224	arg1	stability					226:234	mechanical and chemical stability	202:234	mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS)	202:297	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	0	37	theme	layered	19:25	arg1	hydroxide					34:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide	0:42	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	2	38	theme	glycol-assisted	535:549	arg1	method					564:569	one-pot ethylene glycol-assisted solvothermal method	518:569	one-pot ethylene glycol-assisted solvothermal method	518:569	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	4	39	theme	25.7	930:933	arg1	mS					935:936	mS	935:936	mS	935:936	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	2	40	theme	hierarchical	442:453	arg1	LDHs					494:497	LDHs	494:497	LDHs	494:497	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	40	theme	hierarchical	442:453	arg1	hydroxides					482:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides	419:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs)	419:498	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	41	theme	layered	467:473	arg1	LDHs					494:497	LDHs	494:497	LDHs	494:497	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	41	theme	layered	467:473	arg1	hydroxides					482:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides	419:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs)	419:498	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	42	theme	ethylene	526:533	arg1	method					564:569	one-pot ethylene glycol-assisted solvothermal method	518:569	one-pot ethylene glycol-assisted solvothermal method	518:569	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	43	theme	flower-like	455:465	arg1	LDHs					494:497	LDHs	494:497	LDHs	494:497	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	43	theme	flower-like	455:465	arg1	hydroxides					482:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides	419:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs)	419:498	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	44	theme	one-pot	518:524	arg1	method					564:569	one-pot ethylene glycol-assisted solvothermal method	518:569	one-pot ethylene glycol-assisted solvothermal method	518:569	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	1	45	theme	mechanical	379:388	arg1	strength					390:397	mechanical strength	379:397	mechanical strength of QCS	379:404	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	2	46	theme	three-dimensional	419:435	arg1	LDHs					494:497	LDHs	494:497	LDHs	494:497	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	2	46	theme	three-dimensional	419:435	arg1	hydroxides					482:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides	419:491	three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs)	419:498	Afterwards, three-dimensional (3D) hierarchical flower-like layered double hydroxides (LDHs) were prepared via one-pot ethylene glycol-assisted solvothermal method, and then were incorporated into QCS/PVA blend matrix to fabricate composite AEMs.					
33483019	5	47	theme	cm-2	1318:1321	arg1	density					1301:1307	a peak power density	1288:1307	a peak power density of 73 mW cm-2	1288:1321	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	48	theme	anion	239:243	arg1	AEMs					265:268	AEMs	265:268	AEMs	265:268	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	48	theme	anion	239:243	arg1	membranes					254:262	anion exchange membranes	239:262	anion exchange membranes (AEMs)	239:269	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	48	theme	anion	239:243	arg1	chitosan					284:291	quaternized chitosan	272:291	quaternized chitosan (QCS)	272:297	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	0	49	theme	chitosan/polyvinyl	65:82	arg1	membranes					119:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes	53:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells	53:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	5	50	theme	residual	1076:1083	arg1	conductivity					1091:1102	residual ionic conductivity	1076:1102	residual ionic conductivity of as high as 92 % after immersion	1076:1137	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	51	theme	Alkaline	1004:1011	arg1	stability					1013:1021	Alkaline stability	1004:1021	Alkaline stability measurement	1004:1033	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	0	52	theme	quaternized	53:63	arg1	membranes					119:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes	53:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells	53:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	3	53	theme	impressive	756:765	arg1	conductivity					771:782	impressive ion conductivity	756:782	impressive ion conductivity	756:782	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	54	theme	hierarchical	673:684	arg1	structure					686:694	3D hierarchical structure	670:694	3D hierarchical structure	670:694	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	5	55	theme	stability	1013:1021	arg1	measurement					1023:1033	Alkaline stability measurement	1004:1033	Alkaline stability measurement	1004:1033	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	3	56	used	utilized	855:862	arg2	alkali					785:790	alkali	785:790	alkali	785:790	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	56	used	utilized	855:862	arg2	conductivity					771:782	impressive ion conductivity	756:782	impressive ion conductivity	756:782	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	56	used	utilized	855:862	arg2	ability					811:817	fuel resistant ability	796:817	fuel resistant ability	796:817	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	57	theme	ion	767:769	arg1	conductivity					771:782	impressive ion conductivity	756:782	impressive ion conductivity	756:782	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	4	58	theme	OH-	910:912	arg1	higher					961:966	higher	961:966	higher	961:966	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	4	58	theme	OH-	910:912	arg1	conductivity					914:925	the maximum OH- conductivity	898:925	the maximum OH- conductivity	898:925	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	1	59	theme	QCS	402:404	arg1	strength					390:397	mechanical strength	379:397	mechanical strength of QCS	379:404	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	5	60	theme	mW	1315:1316	arg1	cm-2					1318:1321	73 mW cm-2	1312:1321	73 mW cm-2	1312:1321	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	61	theme	composite	1238:1246	arg1	conductivity					1220:1231	conductivity	1220:1231	conductivity	1220:1231	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	61	theme	composite	1238:1246	arg1	membrane					1248:1255	the composite membrane	1234:1255	the composite membrane	1234:1255	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	62	theme	exchange	245:252	arg1	AEMs					265:268	AEMs	265:268	AEMs	265:268	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	62	theme	exchange	245:252	arg1	membranes					254:262	anion exchange membranes	239:262	anion exchange membranes (AEMs)	239:269	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	1	62	theme	exchange	245:252	arg1	chitosan					284:291	quaternized chitosan	272:291	quaternized chitosan (QCS)	272:297	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	4	63	theme	maximum	902:908	arg1	higher					961:966	higher	961:966	higher	961:966	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	4	63	theme	maximum	902:908	arg1	conductivity					914:925	the maximum OH- conductivity	898:925	the maximum OH- conductivity	898:925	The composite membrane displayed the maximum OH- conductivity of 25.7 mS cm-1, which was 48.6 % higher than that of the pristine membrane.					
33483019	1	64	theme	membranes	254:262	arg1	stability					226:234	mechanical and chemical stability	202:234	mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS)	202:297	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	0	65	theme	composite	92:100	arg1	membranes					119:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes	53:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells	53:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	3	66	theme	3D	670:671	arg1	structure					686:694	3D hierarchical structure	670:694	3D hierarchical structure	670:694	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	5	67	theme	%	1121:1121	arg1	conductivity					1091:1102	residual ionic conductivity	1076:1102	residual ionic conductivity of as high as 92 % after immersion	1076:1137	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	1	68	theme	trade-off	158:166	arg1	problem					168:174	the trade-off problem	154:174	the trade-off problem among ionic conductivity	154:199	To solve the trade-off problem among ionic conductivity, mechanical and chemical stability of anion exchange membranes (AEMs), quaternized chitosan (QCS) was first prepared and then was blended with polyvinyl alcohol (PVA) to improve mechanical strength of QCS.					
33483019	5	69	theme	methanol	1184:1191	arg1	permeability					1193:1204	the decreased methanol permeability	1170:1204	the decreased methanol permeability	1170:1204	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	0	70	theme	alcohol	84:90	arg1	membranes					119:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes	53:127	quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells	53:142	3D nanoflower-like layered double hydroxide modified quaternized chitosan/polyvinyl alcohol composite anion conductive membranes for fuel cells.					
33483019	3	71	theme	active	701:706	arg1	sites					708:712	the active sites	697:712	the active sites of LDH nanosheets	697:730	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	3	71	theme	active	701:706	arg1	nanosheets					721:730	LDH nanosheets	717:730	LDH nanosheets	717:730	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	5	72	theme	decreased	1174:1182	arg1	permeability					1193:1204	the decreased methanol permeability	1170:1204	the decreased methanol permeability	1170:1204	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	73	theme	73	1312:1313	arg1	mW					1315:1316	mW	1315:1316	mW	1315:1316	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	3	74	theme	fuel	796:799	arg1	ability					811:817	fuel resistant ability	796:817	fuel resistant ability	796:817	By constructing 3D hierarchical structure, the active sites of LDH nanosheets are fully exposed, thus impressive ion conductivity, alkali and fuel resistant ability of LDH nanosheets can be rationally utilized.					
33483019	5	75	dep	membranes	1061:1069	arg1	kept					1071:1074	kept	1071:1074	kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability	1071:1204	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
33483019	5	75	dep	membranes	1061:1069	arg1	increased					1210:1218	increased	1210:1218	increased conductivity, the composite membrane with 6% LDHs content	1210:1276	Alkaline stability measurement proved that the composite membranes kept residual ionic conductivity of as high as 92 % after immersion in a 2 M KOH for 100 h. Due to the decreased methanol permeability and increased conductivity, the composite membrane with 6% LDHs content exhibited a peak power density of 73 mW cm-2 at 60 °C, whereas the pristine membrane demonstrated only 40 mW cm-2.					
32484938	10	0	theme	landraces	1769:1777	arg1	genome					1746:1751	genome	1746:1751	genome of these barley landraces to evolve some very specific traits	1746:1813	The landraces analyzed in this study have remained intact for the last 900 years, which gives opportunity to genome of these barley landraces to evolve some very specific traits.					
32484938	3	1	theme	barley	691:696	arg1	landraces					698:706	the whitish or lightly pigmented barley landraces	658:706	the whitish or lightly pigmented barley landraces	658:706	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	4	2	theme	barley	824:829	arg1	type					816:819	type	816:819	type of barley (landrace or commercial) and number of rows	816:873	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	7	3	theme	origin	1360:1365	arg1	island					1350:1355	the island	1346:1355	the island of origin	1346:1365	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	8	4	dep	human	1441:1445	arg1	feed					1458:1461	feed	1458:1461	feed	1458:1461	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	4	5	theme	number	860:865	arg1	type					816:819	type	816:819	type of barley (landrace or commercial) and number of rows	816:873	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	6	6	theme	barley	1191:1196	arg1	grains					1198:1203	the barley grains	1187:1203	the barley grains	1187:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	3	7	theme	six-row	529:535	arg1	landraces					544:552	the six-row barley landraces	525:552	the six-row barley landraces with strongly pigmented grains	525:583	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	6	8	theme	mean	1107:1110	arg1	values					1112:1117	the mean values	1103:1117	the mean values in all the chemical parameters according to the island of origin of the barley grains	1103:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	4	9	used	used	763:766	arg2	analysis					749:756	linear discriminant analysis	729:756	linear discriminant analysis	729:756	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	4	9	used	used	763:766	arg2	analysis					716:723	Factor analysis	709:723	Factor analysis	709:723	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	2	10	theme	fiber	489:493	arg1	concentrations					495:508	higher mean ash and fiber concentrations	469:508	concentrations	495:508	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	3	11	dep	lower	589:593	arg1	manganese					604:612	manganese	604:612	manganese	604:612	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	3	11	dep	lower	589:593	arg1	zinc					595:598	zinc	595:598	zinc	595:598	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	9	12	from	protein	1553:1559	arg1	rich					1537:1540	rich	1537:1540	rich	1537:1540	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	6	13	theme	origin	1177:1182	arg1	island					1167:1172	the island	1163:1172	the island of origin of the barley grains	1163:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	9	14	from	starch	1545:1550	arg1	rich					1537:1540	rich	1537:1540	rich	1537:1540	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	11	15	theme	new	1932:1934	arg1	quality					1936:1942	new quality	1932:1942	new quality	1932:1942	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	7	16	theme	barley	1302:1307	arg1	landraces					1309:1317	all the barley landraces	1294:1317	all the barley landraces with six rows according to the island of origin	1294:1365	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	3	17	theme	lower	589:593	arg1	concentrations					638:651	lower zinc and manganese and higher protein mean concentrations	589:651	lower zinc and manganese and higher protein mean concentrations	589:651	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	11	18	theme	breeding	1947:1954	arg1	programs					1956:1963	breeding programs	1947:1963	breeding programs	1947:1963	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	1	19	theme	total	124:128	arg1	phenolics					130:138	total phenolics	124:138	total phenolics	124:138	The proximate composition, total phenolics, antioxidant activity, minerals, and trace elements were determined in 42 barley landraces in order to highlight their nutritional potential and promote their cultivation.					
32484938	2	20	theme	six-row	441:447	arg1	ones					449:452	six-row ones	441:452	six-row ones that presented higher mean ash and fiber concentrations	441:508	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	0	21	from	composition	13:23	arg1	Islands					88:94	the Canary Islands	77:94	the Canary Islands	77:94	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	11	22	theme	quality	1936:1942	arg1	source					1922:1927	a source	1920:1927	a source of new quality in breeding programs	1920:1963	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	7	23	with	landraces	1309:1317	arg1	rows					1328:1331	six rows	1324:1331	six rows according to the island of origin	1324:1365	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	11	24	theme	landraces	1868:1876	arg1	characterization					1836:1851	The physicochemical characterization	1816:1851	The physicochemical characterization of these local landraces carried out by us	1816:1894	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	11	24	theme	landraces	1868:1876	arg1	useful					1910:1915	useful	1910:1915	useful	1910:1915	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	4	25	theme	discriminant	736:747	arg1	analysis					749:756	linear discriminant analysis	729:756	linear discriminant analysis	729:756	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	11	26	from	source	1922:1927	arg1	programs					1956:1963	breeding programs	1947:1963	breeding programs	1947:1963	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	3	27	with	landraces	544:552	arg1	grains					578:583	strongly pigmented grains	559:583	strongly pigmented grains	559:583	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	5	28	theme	phenolic	1044:1051	arg1	antioxidants					1053:1064	phenolic antioxidants	1044:1064	phenolic antioxidants	1044:1064	In general, all the parameters varied considerably among the barley landraces analyzed, but some barley landraces could be emphasized according to fiber, minerals, and phenolic antioxidants.					
32484938	2	29	theme	higher	469:474	arg1	ash					481:483	higher mean ash and fiber concentrations	469:508	ash	481:483	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	2	30	theme	starch	369:374	arg1	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	0	31	theme	Canary	81:86	arg1	Islands					88:94	the Canary Islands	77:94	the Canary Islands	77:94	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	4	32	theme	Factor	709:714	arg1	analysis					716:723	Factor analysis	709:723	Factor analysis	709:723	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	2	33	theme	compounds	401:409	arg1	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	3	34	theme	pigmented	568:576	arg1	grains					578:583	strongly pigmented grains	559:583	strongly pigmented grains	559:583	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	5	35	theme	barley	973:978	arg1	landraces					980:988	some barley landraces	968:988	some barley landraces	968:988	In general, all the parameters varied considerably among the barley landraces analyzed, but some barley landraces could be emphasized according to fiber, minerals, and phenolic antioxidants.					
32484938	2	36	theme	total	386:390	arg1	compounds					401:409	total phenolic compounds	386:409	total phenolic compounds	386:409	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	0	37	dep	vulgare	50:56	arg1	L.					58:59	Hordeum vulgare L.	42:59	Hordeum vulgare L.	42:59	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	6	38	from	values	1112:1117	arg1	parameters					1139:1148	all the chemical parameters	1122:1148	all the chemical parameters according to the island of origin of the barley grains	1122:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	2	39	theme	protein	377:383	arg1	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	0	40	theme	barley	28:33	arg1	grain					35:39	barley grain	28:39	barley grain (Hordeum vulgare L.) landraces from the Canary Islands	28:94	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	0	40	theme	barley	28:33	arg1	vulgare					50:56	Hordeum vulgare L.	42:59	Hordeum vulgare L.	42:59	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	2	41	theme	barley	320:325	arg1	landraces					327:335	Two-row barley landraces	312:335	Two-row barley landraces	312:335	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	9	42	theme	view	1521:1524	arg1	point					1512:1516	the nutritional point	1496:1516	the nutritional point of view	1496:1524	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	7	43	theme	discriminant	1232:1243	arg1	analysis					1245:1252	linear discriminant analysis	1225:1252	linear discriminant analysis	1225:1252	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	6	44	from	differences	1088:1098	arg1	values					1112:1117	the mean values	1103:1117	the mean values in all the chemical parameters according to the island of origin of the barley grains	1103:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	3	45	theme	protein	625:631	arg1	concentrations					638:651	lower zinc and manganese and higher protein mean concentrations	589:651	lower zinc and manganese and higher protein mean concentrations	589:651	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	2	46	theme	higher	343:348	arg1	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	3	47	contain	had	585:587	arg2	concentrations					638:651	lower zinc and manganese and higher protein mean concentrations	589:651	lower zinc and manganese and higher protein mean concentrations	589:651	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	3	47	contain	had	585:587	arg1	landraces					544:552	the six-row barley landraces	525:552	the six-row barley landraces with strongly pigmented grains	525:583	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	1	48	theme	barley	214:219	arg1	landraces					221:229	42 barley landraces	211:229	42 barley landraces in order to highlight their nutritional potential and promote their cultivation	211:309	The proximate composition, total phenolics, antioxidant activity, minerals, and trace elements were determined in 42 barley landraces in order to highlight their nutritional potential and promote their cultivation.					
32484938	10	49	theme	barley	1762:1767	arg1	landraces					1769:1777	these barley landraces	1756:1777	these barley landraces	1756:1777	The landraces analyzed in this study have remained intact for the last 900 years, which gives opportunity to genome of these barley landraces to evolve some very specific traits.					
32484938	6	50	theme	chemical	1130:1137	arg1	parameters					1139:1148	all the chemical parameters	1122:1148	all the chemical parameters according to the island of origin of the barley grains	1122:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	10	51	theme	specific	1799:1806	arg1	traits					1808:1813	some very specific traits	1789:1813	some very specific traits	1789:1813	The landraces analyzed in this study have remained intact for the last 900 years, which gives opportunity to genome of these barley landraces to evolve some very specific traits.					
32484938	1	52	theme	antioxidant	141:151	arg1	activity					153:160	antioxidant activity	141:160	antioxidant activity	141:160	The proximate composition, total phenolics, antioxidant activity, minerals, and trace elements were determined in 42 barley landraces in order to highlight their nutritional potential and promote their cultivation.					
32484938	9	53	theme	dietary	1562:1568	arg1	fiber					1570:1574	dietary fiber	1562:1574	dietary fiber	1562:1574	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	3	54	theme	pigmented	681:689	arg1	landraces					698:706	the whitish or lightly pigmented barley landraces	658:706	the whitish or lightly pigmented barley landraces	658:706	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	6	55	theme	grains	1198:1203	arg1	origin					1177:1182	origin	1177:1182	origin of the barley grains	1177:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	9	56	from	fiber	1570:1574	arg1	rich					1537:1540	rich	1537:1540	rich	1537:1540	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	11	57	theme	physicochemical	1820:1834	arg1	characterization					1836:1851	The physicochemical characterization	1816:1851	The physicochemical characterization of these local landraces carried out by us	1816:1894	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	11	57	theme	physicochemical	1820:1834	arg1	useful					1910:1915	useful	1910:1915	useful	1910:1915	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	1	58	theme	trace	177:181	arg1	elements					183:190	trace elements	177:190	trace elements	177:190	The proximate composition, total phenolics, antioxidant activity, minerals, and trace elements were determined in 42 barley landraces in order to highlight their nutritional potential and promote their cultivation.					
32484938	3	59	theme	barley	537:542	arg1	landraces					544:552	the six-row barley landraces	525:552	the six-row barley landraces with strongly pigmented grains	525:583	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	8	60	dep	APPLICATION	1378:1388	arg1	cereal					1413:1418	cereal	1413:1418	cereal	1413:1418	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	8	60	dep	APPLICATION	1378:1388	arg1	Barley					1391:1396	Barley	1391:1396	Barley	1391:1396	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	8	60	dep	APPLICATION	1378:1388	arg1	biodiesel					1480:1488	biodiesel	1480:1488	biodiesel	1480:1488	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	9	61	theme	antioxidant	1601:1611	arg1	compounds					1613:1621	antioxidant compounds	1601:1621	antioxidant compounds	1601:1621	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	0	62	from	Islands	88:94	arg1	composition					13:23	The chemical composition	0:23	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.	0:95	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	0	62	from	Islands	88:94	arg1	landraces					62:70	barley grain (Hordeum vulgare L.) landraces	28:70	barley grain (Hordeum vulgare L.) landraces from the Canary Islands	28:94	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	11	63	theme	local	1862:1866	arg1	landraces					1868:1876	these local landraces	1856:1876	these local landraces carried out by us	1856:1894	The physicochemical characterization of these local landraces carried out by us could be very useful as a source of new quality in breeding programs.					
32484938	1	64	theme	proximate	101:109	arg1	composition					111:121	The proximate composition	97:121	The proximate composition	97:121	The proximate composition, total phenolics, antioxidant activity, minerals, and trace elements were determined in 42 barley landraces in order to highlight their nutritional potential and promote their cultivation.					
32484938	4	65	theme	rows	870:873	arg1	number					860:865	number	860:865	number of rows	860:873	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	4	65	theme	rows	870:873	arg1	barley					824:829	barley	824:829	barley (landrace or commercial)	824:854	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	10	66	theme	last	1703:1706	arg1	years					1712:1716	the last 900 years	1699:1716	the last 900 years	1699:1716	The landraces analyzed in this study have remained intact for the last 900 years, which gives opportunity to genome of these barley landraces to evolve some very specific traits.					
32484938	8	67	used	used	1432:1435	arg2	cereal					1413:1418	cereal	1413:1418	cereal	1413:1418	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	8	67	used	used	1432:1435	arg2	biodiesel					1480:1488	biodiesel	1480:1488	biodiesel	1480:1488	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	8	67	used	used	1432:1435	arg2	Barley					1391:1396	Barley	1391:1396	Barley	1391:1396	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32484938	0	68	theme	landraces	62:70	arg1	composition					13:23	The chemical composition	0:23	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.	0:95	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	9	69	from	minerals	1580:1587	arg1	rich					1537:1540	rich	1537:1540	rich	1537:1540	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	2	70	theme	mean	476:479	arg1	ash					481:483	higher mean ash and fiber concentrations	469:508	ash	481:483	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	4	71	theme	linear	729:734	arg1	analysis					749:756	linear discriminant analysis	729:756	linear discriminant analysis	729:756	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	2	72	contain	had	337:339	arg2	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	2	72	contain	had	337:339	arg1	landraces					327:335	Two-row barley landraces	312:335	Two-row barley landraces	312:335	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	5	73	theme	barley	937:942	arg1	landraces					944:952	the barley landraces	933:952	the barley landraces analyzed	933:961	In general, all the parameters varied considerably among the barley landraces analyzed, but some barley landraces could be emphasized according to fiber, minerals, and phenolic antioxidants.					
32484938	9	74	from	rich	1537:1540	arg1	minerals					1580:1587	minerals	1580:1587	minerals	1580:1587	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	9	74	from	rich	1537:1540	arg1	protein					1553:1559	protein	1553:1559	protein	1553:1559	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	9	74	from	rich	1537:1540	arg1	fiber					1570:1574	dietary fiber	1562:1574	dietary fiber	1562:1574	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	9	74	from	rich	1537:1540	arg1	starch					1545:1550	starch	1545:1550	starch	1545:1550	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	0	75	theme	chemical	4:11	arg1	composition					13:23	The chemical composition	0:23	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.	0:95	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	7	76	theme	useful	1265:1270	arg1	tool					1272:1275	a useful tool	1263:1275	a useful tool to differentiate all the barley landraces with six rows according to the island of origin	1263:1365	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	7	76	theme	useful	1265:1270	arg1	application					1210:1220	The application	1206:1220	The application of linear discriminant analysis	1206:1252	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	2	77	theme	average	350:356	arg1	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	4	78	dep	barley	824:829	arg1	commercial					844:853	commercial	844:853	commercial	844:853	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	4	78	dep	barley	824:829	arg1	landrace					832:839	landrace	832:839	landrace	832:839	Factor analysis and linear discriminant analysis were used to correctly differentiate samples according to type of barley (landrace or commercial) and number of rows.					
32484938	2	79	theme	phenolic	392:399	arg1	compounds					401:409	total phenolic compounds	386:409	total phenolic compounds	386:409	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	0	80	theme	grain	35:39	arg1	landraces					62:70	barley grain (Hordeum vulgare L.) landraces	28:70	barley grain (Hordeum vulgare L.) landraces from the Canary Islands	28:94	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	1	81	theme	nutritional	259:269	arg1	potential					271:279	their nutritional potential	253:279	their nutritional potential	253:279	The proximate composition, total phenolics, antioxidant activity, minerals, and trace elements were determined in 42 barley landraces in order to highlight their nutritional potential and promote their cultivation.					
32484938	9	82	theme	nutritional	1500:1510	arg1	point					1512:1516	the nutritional point	1496:1516	the nutritional point of view	1496:1524	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	9	83	from	point	1512:1516	arg1	rich					1537:1540	rich	1537:1540	rich	1537:1540	From the nutritional point of view, barley is rich in starch, protein, dietary fiber and minerals, as well as antioxidant compounds and vitamins.					
32484938	7	84	theme	analysis	1245:1252	arg1	tool					1272:1275	a useful tool	1263:1275	a useful tool to differentiate all the barley landraces with six rows according to the island of origin	1263:1365	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	7	84	theme	analysis	1245:1252	arg1	application					1210:1220	The application	1206:1220	The application of linear discriminant analysis	1206:1252	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	0	85	theme	Hordeum	42:48	arg1	grain					35:39	barley grain	28:39	barley grain (Hordeum vulgare L.) landraces from the Canary Islands	28:94	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	0	85	theme	Hordeum	42:48	arg1	vulgare					50:56	Hordeum vulgare L.	42:59	Hordeum vulgare L.	42:59	The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands.					
32484938	7	86	theme	linear	1225:1230	arg1	analysis					1245:1252	linear discriminant analysis	1225:1252	linear discriminant analysis	1225:1252	The application of linear discriminant analysis was also a useful tool to differentiate all the barley landraces with six rows according to the island of origin.					
32484938	2	87	theme	iron	416:419	arg1	content					358:364	a higher average content	341:364	a higher average content of starch, protein, total phenolic compounds, and iron	341:419	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	3	88	theme	mean	633:636	arg1	concentrations					638:651	lower zinc and manganese and higher protein mean concentrations	589:651	lower zinc and manganese and higher protein mean concentrations	589:651	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	2	89	theme	Two-row	312:318	arg1	landraces					327:335	Two-row barley landraces	312:335	Two-row barley landraces	312:335	Two-row barley landraces had a higher average content of starch, protein, total phenolic compounds, and iron, when compared with six-row ones that presented higher mean ash and fiber concentrations.					
32484938	6	90	theme	important	1078:1086	arg1	differences					1088:1098	important differences	1078:1098	important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains	1078:1203	There were important differences in the mean values in all the chemical parameters according to the island of origin of the barley grains.					
32484938	3	91	theme	higher	618:623	arg1	concentrations					638:651	lower zinc and manganese and higher protein mean concentrations	589:651	lower zinc and manganese and higher protein mean concentrations	589:651	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	3	92	theme	whitish	662:668	arg1	landraces					698:706	the whitish or lightly pigmented barley landraces	658:706	the whitish or lightly pigmented barley landraces	658:706	Additionally, the six-row barley landraces with strongly pigmented grains had lower zinc and manganese and higher protein mean concentrations than the whitish or lightly pigmented barley landraces.					
32484938	8	93	theme	PRACTICAL	1368:1376	arg1	APPLICATION					1378:1388	PRACTICAL APPLICATION	1368:1388	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.	1368:1489	PRACTICAL APPLICATION: Barley is a versatile cereal that can be used for human and animal feed, brewing, and as biodiesel.					
32387606	4	0	theme	fabricated	704:713	arg1	hydrogel					715:722	the fabricated hydrogel	700:722	the fabricated hydrogel	700:722	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	1	1	theme	biodegradable	155:167	arg1	scaffold					169:176	a biodegradable scaffold	153:176	a biodegradable scaffold	153:176	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	1	2	theme	growth	239:244	arg1	factors					246:252	growth factors	239:252	growth factors	239:252	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	0	3	theme	composite	88:96	arg1	hydrogel					98:105	non-biodegradable bacterial nanocellulose composite hydrogel	46:105	non-biodegradable bacterial nanocellulose composite hydrogel on wound healing	46:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	5	4	theme	wound	877:881	arg1	healing					883:889	faster wound healing	870:889	faster wound healing	870:889	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	3	5	theme	BNC/AA	531:536	arg1	hydrogel					539:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	6	theme	HDF	653:655	arg1	wound					598:602	wound	598:602	wound	598:602	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	6	theme	HDF	653:655	arg1	effects					630:636	the resulting healing effects	608:636	the resulting healing effects	608:636	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	0	7	theme	wound	110:114	arg1	healing					116:122	wound healing	110:122	wound healing	110:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	3	8	theme	human	560:564	arg1	HDF					586:588	HDF	586:588	HDF	586:588	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	8	theme	human	560:564	arg1	fibroblasts					573:583	human dermal fibroblasts	560:583	human dermal fibroblasts (HDF)	560:589	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	6	9	theme	skin	1118:1121	arg1	engineering					1130:1140	skin tissue engineering	1118:1140	skin tissue engineering	1118:1140	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	5	10	theme	mature	945:950	arg1	microstructure					970:983	more mature newly formed skin microstructure	940:983	more mature newly formed skin microstructure	940:983	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	3	11	theme	dermal	566:571	arg1	HDF					586:588	HDF	586:588	HDF	586:588	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	11	theme	dermal	566:571	arg1	fibroblasts					573:583	human dermal fibroblasts	560:583	human dermal fibroblasts (HDF)	560:589	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	6	12	theme	new	1049:1051	arg1	perspective					1053:1063	a new perspective	1047:1063	a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level	1047:1207	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	5	13	theme	protein	847:853	arg1	study					855:859	The gene and protein study	834:859	study	855:859	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	5	14	theme	gene	838:841	arg1	study					855:859	The gene and protein study	834:859	study	855:859	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	6	15	theme	non-biodegradable	1087:1103	arg1	materials					1105:1113	non-biodegradable materials	1087:1113	non-biodegradable materials	1087:1113	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	5	16	theme	formed	958:963	arg1	microstructure					970:983	more mature newly formed skin microstructure	940:983	more mature newly formed skin microstructure	940:983	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	5	17	theme	HDF	912:914	arg1	group					916:920	HDF group	912:920	HDF group	912:920	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	3	18	from	effects	630:636	arg1	mice					668:671	athymic mice	660:671	athymic mice	660:671	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	19	dep	wound	598:602	arg1	the					594:596	the	594:596	the	594:596	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	1	20	theme	extracellular	259:271	arg1	matrix					273:278	extracellular matrix	259:278	extracellular matrix	259:278	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	4	21	theme	wound	770:774	arg1	site					776:779	the wound site	766:779	the wound site within 24 h	766:791	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	5	22	dep	group	916:920	arg1	the					894:896	the	894:896	the	894:896	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	5	22	dep	group	916:920	arg1	hydrogel					898:905	hydrogel	898:905	hydrogel	898:905	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	0	23	from	hydrogel	98:105	arg1	healing					116:122	wound healing	110:122	wound healing	110:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	4	24	theme	HDF	757:759	arg1	HDF					757:759	HDF	757:759	HDF	757:759	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	4	24	theme	HDF	757:759	arg1	%					752:752	>50%	749:752	>50% of HDF	749:759	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	3	25	from	wound	598:602	arg1	mice					668:671	athymic mice	660:671	athymic mice	660:671	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	0	26	theme	fibroblast	32:41	arg1	delivery					13:20	delivery	13:20	delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing	13:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	3	27	theme	acid	525:528	arg1	hydrogel					539:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	6	28	theme	materials	1105:1113	arg1	role					1079:1082	the role	1075:1082	the role of non-biodegradable materials in skin tissue engineering	1075:1140	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	4	29	theme	HDF	811:813	arg1	evidence					799:806	evidence	799:806	evidence of HDF detected on day 7	799:831	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	6	30	theme	exogenous	1162:1170	arg1	cells					1172:1176	exogenous cells	1162:1176	exogenous cells	1162:1176	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	2	31	theme	scaffold	403:410	arg1	role					377:380	the role	373:380	the role of non-biodegradable scaffold	373:410	However, the role of non-biodegradable scaffold remains unexplored.					
32387606	0	32	theme	dermal	25:30	arg1	fibroblast					32:41	dermal fibroblast	25:41	dermal fibroblast	25:41	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	6	33	theme	molecular	1193:1201	arg1	level					1203:1207	the molecular level	1189:1207	the molecular level	1189:1207	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	2	34	theme	non-biodegradable	385:401	arg1	scaffold					403:410	non-biodegradable scaffold	385:410	non-biodegradable scaffold	385:410	However, the role of non-biodegradable scaffold remains unexplored.					
32387606	0	35	theme	non-biodegradable	46:62	arg1	hydrogel					98:105	non-biodegradable bacterial nanocellulose composite hydrogel	46:105	non-biodegradable bacterial nanocellulose composite hydrogel on wound healing	46:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	1	36	theme	regenerated	291:301	arg1	tissue					303:308	the regenerated tissue	287:308	the regenerated tissue	287:308	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	3	37	theme	transferred	641:651	arg1	HDF					653:655	transferred HDF	641:655	transferred HDF	641:655	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	38	theme	nanocellulose/acrylic	503:523	arg1	hydrogel					539:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	3	39	theme	non-biodegradable	475:491	arg1	hydrogel					539:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	5	40	theme	visible	1006:1012	arg1	differences					1014:1024	no visible differences	1003:1024	no visible differences	1003:1024	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	1	41	theme	skin	128:131	arg1	engineering					140:150	skin tissue engineering	128:150	skin tissue engineering	128:150	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	3	42	theme	resulting	612:620	arg1	effects					630:636	the resulting healing effects	608:636	the resulting healing effects	608:636	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	5	43	theme	skin	965:968	arg1	microstructure					970:983	more mature newly formed skin microstructure	940:983	more mature newly formed skin microstructure	940:983	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	3	44	theme	healing	622:628	arg1	effects					630:636	the resulting healing effects	608:636	the resulting healing effects	608:636	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	1	45	theme	tissue	133:138	arg1	engineering					140:150	skin tissue engineering	128:150	skin tissue engineering	128:150	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	6	46	theme	tissue	1123:1128	arg1	engineering					1130:1140	skin tissue engineering	1118:1140	skin tissue engineering	1118:1140	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	3	47	theme	athymic	660:666	arg1	mice					668:671	athymic mice	660:671	athymic mice	660:671	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	1	48	used	used	189:192	arg2	scaffold					169:176	a biodegradable scaffold	153:176	a biodegradable scaffold	153:176	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	3	49	theme	bacterial	493:501	arg1	hydrogel					539:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel	473:546	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32387606	1	50	theme	own	224:226	arg1	cytokines					228:236	its own cytokines	220:236	its own cytokines	220:236	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	0	51	theme	nanocellulose	74:86	arg1	hydrogel					98:105	non-biodegradable bacterial nanocellulose composite hydrogel	46:105	non-biodegradable bacterial nanocellulose composite hydrogel on wound healing	46:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	6	52	theme	cells	1172:1176	arg1	presence					1150:1157	the presence	1146:1157	the presence of exogenous cells	1146:1176	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	5	53	theme	faster	870:875	arg1	healing					883:889	faster wound healing	870:889	faster wound healing	870:889	The gene and protein study unveiled faster wound healing in the hydrogel with HDF group and characterized more mature newly formed skin microstructure on day 7, despite no visible differences.					
32387606	4	54	located	detected	815:822	arg1	day					827:829	day 7	827:831	day 7	827:831	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	4	54	located	detected	815:822	arg2	HDF					811:813	HDF	811:813	HDF detected on day 7	811:831	Results demonstrated that the fabricated hydrogel successfully transferred >50% of HDF onto the wound site within 24 h, with evidence of HDF detected on day 7.					
32387606	0	55	theme	bacterial	64:72	arg1	hydrogel					98:105	non-biodegradable bacterial nanocellulose composite hydrogel	46:105	non-biodegradable bacterial nanocellulose composite hydrogel on wound healing	46:122	Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing.					
32387606	1	56	dep	grow	206:209	arg1	produce					212:218	produce	212:218	produce its own cytokines, growth factors, and extracellular matrix	212:278	In skin tissue engineering, a biodegradable scaffold is usually used where cells grow, produce its own cytokines, growth factors, and extracellular matrix, until the regenerated tissue gradually replaces the scaffold upon its degradation.					
32387606	6	57	from	role	1079:1082	arg1	engineering					1130:1140	skin tissue engineering	1118:1140	skin tissue engineering	1118:1140	These findings give a new perspective regarding the role of non-biodegradable materials in skin tissue engineering, in the presence of exogenous cells, mainly at the molecular level.					
32387606	3	58	theme	hydrogel	539:546	arg1	potential					460:468	the potential	456:468	the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice	456:671	This study investigates the potential of a non-biodegradable bacterial nanocellulose/acrylic acid (BNC/AA) hydrogel to transfer human dermal fibroblasts (HDF) to the wound and the resulting healing effects of transferred HDF in athymic mice.					
32933697	5	0	theme	reported	993:1000	arg1	probes					1014:1019	other reported fluorescent probes	987:1019	other reported fluorescent probes	987:1019	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	4	1	theme	inner	802:806	arg1	IFE					823:825	IFE	823:825	IFE	823:825	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	4	1	theme	inner	802:806	arg1	effect					815:820	the inner filter effect	798:820	the inner filter effect (IFE)	798:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	0	2	theme	cell	73:76	arg1	imaging					78:84	live cell imaging	68:84	live cell imaging	68:84	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	5	3	theme	fluorescent	1002:1012	arg1	probes					1014:1019	other reported fluorescent probes	987:1019	other reported fluorescent probes	987:1019	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	0	4	dep	Cr	113:114	arg1	VI					116:117	VI	116:117	VI	116:117	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	0	5	theme	sensitive	90:98	arg1	detection					100:108	sensitive detection	90:108	sensitive detection	90:108	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	6	6	theme	practical	1078:1086	arg1	detection					1088:1096	practical detection	1078:1096	practical detection of Cr(VI) and AA	1078:1113	In addition, the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA was also studied.					
32933697	5	7	theme	linear	907:912	arg1	range					914:918	linear range	907:918	linear range	907:918	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	4	8	theme	selective	736:744	arg1	detection					746:754	sensitive and selective detection	722:754	sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE)	722:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	4	9	theme	detection	622:630	arg1	experiments					632:642	Fluorescence detection experiments	609:642	Fluorescence detection experiments	609:642	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	1	10	theme	big	221:223	arg1	challenge					225:233	a big challenge	219:233	a big challenge	219:233	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	3	11	theme	low	529:531	arg1	dispersibility					457:470	high aqueous dispersibility	444:470	high aqueous dispersibility	444:470	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	11	theme	low	529:531	arg1	cytotoxicity					533:544	low cytotoxicity	529:544	low cytotoxicity	529:544	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	4	12	theme	filter	808:813	arg1	IFE					823:825	IFE	823:825	IFE	823:825	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	4	12	theme	filter	808:813	arg1	effect					815:820	the inner filter effect	798:820	the inner filter effect (IFE)	798:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	3	13	theme	stable	505:510	arg1	dispersibility					457:470	high aqueous dispersibility	444:470	high aqueous dispersibility	444:470	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	13	theme	stable	505:510	arg1	fluorescence					512:523	stable fluorescence	505:523	stable fluorescence	505:523	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	14	theme	tunable	487:493	arg1	emission					495:502	tunable emission	487:502	tunable emission	487:502	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	14	theme	tunable	487:493	arg1	dispersibility					457:470	high aqueous dispersibility	444:470	high aqueous dispersibility	444:470	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	15	theme	CMC	383:385	arg1	macromolecule					387:399	CMC macromolecule	383:399	CMC macromolecule	383:399	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	16	theme	unique	347:352	arg1	composition					354:364	unique composition	347:364	unique composition	347:364	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	6	17	theme	AA	1112:1113	arg1	detection					1088:1096	practical detection	1078:1096	practical detection of Cr(VI) and AA	1078:1113	In addition, the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA was also studied.					
32933697	3	18	theme	macromolecule	387:399	arg1	structure					370:378	structure	370:378	structure	370:378	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	18	theme	macromolecule	387:399	arg1	composition					354:364	unique composition	347:364	unique composition	347:364	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	19	dep	composition	354:364	arg1	the					343:345	the	343:345	the	343:345	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	2	20	theme	cellulose	277:285	arg1	utilization					248:258	the utilization	244:258	the utilization of carboxymethyl cellulose (CMC) to synthesis of SQDs	244:312	Herein, the utilization of carboxymethyl cellulose (CMC) to synthesis of SQDs is reported.					
32933697	0	21	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.	0:137	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	2	22	theme	carboxymethyl	263:275	arg1	CMC					288:290	CMC	288:290	CMC	288:290	Herein, the utilization of carboxymethyl cellulose (CMC) to synthesis of SQDs is reported.					
32933697	2	22	theme	carboxymethyl	263:275	arg1	cellulose					277:285	carboxymethyl cellulose	263:285	carboxymethyl cellulose (CMC)	263:291	Herein, the utilization of carboxymethyl cellulose (CMC) to synthesis of SQDs is reported.					
32933697	4	23	dep	Cr	759:760	arg1	VI					762:763	VI	762:763	VI	762:763	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	0	24	theme	carboxymethyl	20:32	arg1	dots					59:62	carboxymethyl cellulose sulfur quantum dots	20:62	carboxymethyl cellulose sulfur quantum dots	20:62	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	4	25	theme	sensitive	722:730	arg1	detection					746:754	sensitive and selective detection	722:754	sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE)	722:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	1	26	theme	stable	165:170	arg1	SQDs					205:208	SQDs	205:208	SQDs	205:208	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	1	26	theme	stable	165:170	arg1	dots					199:202	stable fluorescent sulfur quantum dots	165:202	stable fluorescent sulfur quantum dots (SQDs)	165:209	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	4	27	theme	on-off-on	702:710	arg1	CMC-SQDs					663:670	the CMC-SQDs	659:670	the CMC-SQDs	659:670	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	4	27	theme	on-off-on	702:710	arg1	switch					712:717	a fluorescence on-off-on switch	687:717	a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE)	687:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	6	28	theme	fluorescent	1053:1063	arg1	CMC-SQDs					1065:1072	fluorescent CMC-SQDs	1053:1072	fluorescent CMC-SQDs	1053:1072	In addition, the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA was also studied.					
32933697	0	29	theme	Cr	113:114	arg1	imaging					78:84	live cell imaging	68:84	live cell imaging	68:84	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	0	29	theme	Cr	113:114	arg1	detection					100:108	sensitive detection	90:108	sensitive detection	90:108	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	5	30	theme	μM	941:942	arg1	range					914:918	linear range	907:918	linear range	907:918	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	5	31	dep	Cr	860:861	arg1	VI					863:864	VI	863:864	VI	863:864	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	1	32	theme	fluorescent	172:182	arg1	SQDs					205:208	SQDs	205:208	SQDs	205:208	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	1	32	theme	fluorescent	172:182	arg1	dots					199:202	stable fluorescent sulfur quantum dots	165:202	stable fluorescent sulfur quantum dots (SQDs)	165:209	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	0	33	theme	sulfur	44:49	arg1	dots					59:62	carboxymethyl cellulose sulfur quantum dots	20:62	carboxymethyl cellulose sulfur quantum dots	20:62	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	6	34	theme	Cr	1101:1102	arg1	detection					1088:1096	practical detection	1078:1096	practical detection of Cr(VI) and AA	1078:1113	In addition, the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA was also studied.					
32933697	4	35	theme	ascorbic	770:777	arg1	AA					785:786	AA	785:786	AA	785:786	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	4	35	theme	ascorbic	770:777	arg1	acid					779:782	ascorbic acid	770:782	ascorbic acid (AA)	770:787	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	1	36	theme	sulfur	184:189	arg1	SQDs					205:208	SQDs	205:208	SQDs	205:208	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	1	36	theme	sulfur	184:189	arg1	dots					199:202	stable fluorescent sulfur quantum dots	165:202	stable fluorescent sulfur quantum dots (SQDs)	165:209	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	0	37	theme	cellulose	34:42	arg1	dots					59:62	carboxymethyl cellulose sulfur quantum dots	20:62	carboxymethyl cellulose sulfur quantum dots	20:62	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	1	38	theme	quantum	191:197	arg1	SQDs					205:208	SQDs	205:208	SQDs	205:208	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	1	38	theme	quantum	191:197	arg1	dots					199:202	stable fluorescent sulfur quantum dots	165:202	stable fluorescent sulfur quantum dots (SQDs)	165:209	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	0	39	theme	dots	59:62	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.	0:137	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	0	40	theme	ascorbic	124:131	arg1	acid					133:136	ascorbic acid	124:136	ascorbic acid	124:136	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	3	41	theme	fluorescent	590:600	arg1	probe					602:606	a fluorescent probe	588:606	a fluorescent probe	588:606	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	1	42	dep	Nowadays	139:146	arg1	synthesis					152:160	the synthesis	148:160	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs)	139:209	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	3	43	theme	high	444:447	arg1	fluorescence					512:523	stable fluorescence	505:523	stable fluorescence	505:523	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	43	theme	high	444:447	arg1	emission					495:502	tunable emission	487:502	tunable emission	487:502	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	43	theme	high	444:447	arg1	dispersibility					457:470	high aqueous dispersibility	444:470	high aqueous dispersibility	444:470	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	43	theme	high	444:447	arg1	cytotoxicity					533:544	low cytotoxicity	529:544	low cytotoxicity	529:544	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	0	44	theme	quantum	51:57	arg1	dots					59:62	carboxymethyl cellulose sulfur quantum dots	20:62	carboxymethyl cellulose sulfur quantum dots	20:62	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	4	45	theme	acid	779:782	arg1	detection					746:754	sensitive and selective detection	722:754	sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE)	722:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	3	46	theme	aqueous	449:455	arg1	fluorescence					512:523	stable fluorescence	505:523	stable fluorescence	505:523	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	46	theme	aqueous	449:455	arg1	emission					495:502	tunable emission	487:502	tunable emission	487:502	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	46	theme	aqueous	449:455	arg1	dispersibility					457:470	high aqueous dispersibility	444:470	high aqueous dispersibility	444:470	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	3	46	theme	aqueous	449:455	arg1	cytotoxicity					533:544	low cytotoxicity	529:544	low cytotoxicity	529:544	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	4	47	theme	fluorescence	689:700	arg1	CMC-SQDs					663:670	the CMC-SQDs	659:670	the CMC-SQDs	659:670	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	4	47	theme	fluorescence	689:700	arg1	switch					712:717	a fluorescence on-off-on switch	687:717	a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE)	687:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	1	48	theme	dots	199:202	arg1	synthesis					152:160	the synthesis	148:160	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs)	139:209	Nowadays the synthesis of stable fluorescent sulfur quantum dots (SQDs) remains a big challenge.					
32933697	0	49	theme	acid	133:136	arg1	imaging					78:84	live cell imaging	68:84	live cell imaging	68:84	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	0	49	theme	acid	133:136	arg1	detection					100:108	sensitive detection	90:108	sensitive detection	90:108	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	4	50	theme	Cr	759:760	arg1	detection					746:754	sensitive and selective detection	722:754	sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE)	722:826	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	5	51	theme	detection	842:850	arg1	limit					833:837	The limit	829:837	The limit of detection towards Cr(VI) and AA	829:872	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	0	52	theme	live	68:71	arg1	imaging					78:84	live cell imaging	68:84	live cell imaging	68:84	Facile synthesis of carboxymethyl cellulose sulfur quantum dots for live cell imaging and sensitive detection of Cr(VI) and ascorbic acid.					
32933697	3	53	theme	resulted	406:413	arg1	CMC-SQDs					415:422	the resulted CMC-SQDs	402:422	the resulted CMC-SQDs simultaneously	402:437	Benefiting from the unique composition and structure of CMC macromolecule, the resulted CMC-SQDs simultaneously show high aqueous dispersibility and stability, tunable emission, stable fluorescence and low cytotoxicity, which make them promising for working as a fluorescent probe.					
32933697	6	54	dep	Cr	1101:1102	arg1	VI					1104:1105	VI	1104:1105	VI	1104:1105	In addition, the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA was also studied.					
32933697	4	55	theme	Fluorescence	609:620	arg1	experiments					632:642	Fluorescence detection experiments	609:642	Fluorescence detection experiments	609:642	Fluorescence detection experiments suggested that the CMC-SQDs could serve as a fluorescence on-off-on switch to sensitive and selective detection of Cr(VI) and ascorbic acid (AA) based on the inner filter effect (IFE).					
32933697	5	56	theme	other	987:991	arg1	probes					1014:1019	other reported fluorescent probes	987:1019	other reported fluorescent probes	987:1019	The limit of detection towards Cr(VI) and AA can reach 0.024 and 0.18 μM with linear range of 0.5-225 and 1-300 μM, respectively, which compares favorably to other reported fluorescent probes.					
32933697	6	57	theme	CMC-SQDs	1065:1072	arg1	employment					1039:1048	the employment	1035:1048	the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA	1035:1113	In addition, the employment of fluorescent CMC-SQDs for practical detection of Cr(VI) and AA was also studied.					
32933697	2	58	theme	SQDs	309:312	arg1	synthesis					296:304	synthesis	296:304	synthesis of SQDs	296:312	Herein, the utilization of carboxymethyl cellulose (CMC) to synthesis of SQDs is reported.					
32178470	0	0	theme	Gut	127:129	arg1	Composition					142:152	Gut Microbiota Composition	127:152	Gut Microbiota Composition in ICR Mice	127:164	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	9	1	theme	rDNA	1360:1363	arg1	sequencing					1365:1374	the 16S rDNA sequencing	1352:1374	the 16S rDNA sequencing analysis on an IlluminaMiSeq platform	1352:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	8	2	dep	cytokines	1295:1303	arg1	IL-2					1306:1309	IL-2	1306:1309	IL-2	1306:1309	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	2	dep	cytokines	1295:1303	arg1	cytokines					1295:1303	immune-related cytokines	1280:1303	immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α)	1280:1334	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	2	dep	cytokines	1295:1303	arg1	IFN-γ					1318:1322	IFN-γ	1318:1322	IFN-γ	1318:1322	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	2	dep	cytokines	1295:1303	arg1	TNF-α					1329:1333	TNF-α	1329:1333	TNF-α	1329:1333	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	2	dep	cytokines	1295:1303	arg1	IL-6					1312:1315	IL-6	1312:1315	IL-6	1312:1315	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	10	3	theme	increased	1601:1609	arg1	SCP					1597:1599	SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae	1597:1688	SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas	1597:1730	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	9	4	from	results	1341:1347	arg1	platform					1405:1412	an IlluminaMiSeq platform	1388:1412	an IlluminaMiSeq platform	1388:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	6	5	theme	pectin	993:998	arg1	hydrolysates					1000:1011	citrus pectin hydrolysates	986:1011	citrus pectin hydrolysates	986:1011	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	10	6	theme	taxa	1541:1544	arg1	abundance					1520:1528	the relative abundance	1507:1528	the relative abundance of several taxa	1507:1544	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	7	7	theme	immunoregulatory	1057:1072	arg1	properties					1085:1094	immunoregulatory functional properties	1057:1094	immunoregulatory functional properties	1057:1094	Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin.					
32178470	4	8	theme	ICR	680:682	arg1	mice					684:687	immunosuppressed ICR mice	663:687	immunosuppressed ICR mice	663:687	In this research, immunosuppressed ICR mice were administrated with SCP at dosages of 300/600/1200 mg/kg.					
32178470	6	9	theme	citrus	986:991	arg1	hydrolysates					1000:1011	citrus pectin hydrolysates	986:1011	citrus pectin hydrolysates	986:1011	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	5	10	theme	oral	756:759	arg1	gavage					761:766	oral gavage	756:766	oral gavage	756:766	bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined.					
32178470	6	11	theme	water	891:895	arg1	treatment					897:905	subcritical water treatment	879:905	subcritical water treatment	879:905	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	10	12	dep	SCP	1597:1599	arg1	Bacteroidaceae					1655:1668	Bacteroidaceae	1655:1668	Bacteroidaceae	1655:1668	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	10	12	dep	SCP	1597:1599	arg1	Prevotellaceae					1675:1688	Prevotellaceae	1675:1688	Prevotellaceae	1675:1688	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	10	12	dep	SCP	1597:1599	arg1	Ruminococcaceae					1638:1652	Ruminococcaceae	1638:1652	Ruminococcaceae	1638:1652	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	10	12	dep	SCP	1597:1599	arg1	e.g.					1591:1594	e.g.	1591:1594	e.g.	1591:1594	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	9	13	theme	analysis	1376:1383	arg1	results					1341:1347	The results	1337:1347	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform	1337:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	0	14	theme	Microbiota	131:140	arg1	Composition					142:152	Gut Microbiota Composition	127:152	Gut Microbiota Composition in ICR Mice	127:164	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	6	15	theme	subcritical	879:889	arg1	treatment					897:905	subcritical water treatment	879:905	subcritical water treatment	879:905	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	0	16	theme	ICR	157:159	arg1	Mice					161:164	ICR Mice	157:164	ICR Mice	157:164	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	6	17	theme	acid	978:981	arg1	content					954:960	the content	950:960	the content of galacturonic acid in citrus pectin hydrolysates	950:1011	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	8	18	theme	immune-related	1280:1293	arg1	IL-2					1306:1309	IL-2	1306:1309	IL-2	1306:1309	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	18	theme	immune-related	1280:1293	arg1	cytokines					1295:1303	immune-related cytokines	1280:1303	immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α)	1280:1334	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	18	theme	immune-related	1280:1293	arg1	IFN-γ					1318:1322	IFN-γ	1318:1322	IFN-γ	1318:1322	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	18	theme	immune-related	1280:1293	arg1	TNF-α					1329:1333	TNF-α	1329:1333	TNF-α	1329:1333	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	8	18	theme	immune-related	1280:1293	arg1	IL-6					1312:1315	IL-6	1312:1315	IL-6	1312:1315	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	3	19	theme	mouse	632:636	arg1	model					638:642	a cyclophosphamide-induced immunosuppressed mouse model	588:642	a cyclophosphamide-induced immunosuppressed mouse model	588:642	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	3	20	theme	SCP	495:497	arg1	effects					484:490	the effects	480:490	the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis	480:562	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	8	21	dep	organs	1181:1186	arg1	recovery					1201:1208	accelerated recovery	1189:1208	accelerated recovery of immune organ indexes	1189:1232	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	6	22	theme	galacturonic	965:976	arg1	acid					978:981	galacturonic acid	965:981	galacturonic acid	965:981	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	10	23	theme	normal	1576:1581	arg1	status					1583:1588	normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus)	1576:1749	normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus)	1576:1749	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	9	24	from	platform	1405:1412	arg1	results					1341:1347	The results	1337:1347	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform	1337:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	1	25	theme	smaller	223:229	arg1	molecules					231:239	smaller molecules	223:239	smaller molecules	223:239	Subcritical water can effectively hydrolyze pectin into smaller molecules while still maintaining its functional regions.					
32178470	10	26	theme	pathogenic	1707:1716	arg1	Brevundimonas					1718:1730	pathogenic Brevundimonas	1707:1730	pathogenic Brevundimonas	1707:1730	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	10	27	theme	beneficial	1611:1620	arg1	SCP					1597:1599	SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae	1597:1688	SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas	1597:1730	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	8	28	theme	immune	1174:1179	arg1	organs					1181:1186	immune organs	1174:1186	immune organs (accelerated recovery of immune organ indexes)	1174:1233	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	10	29	theme	relative	1511:1518	arg1	abundance					1520:1528	the relative abundance	1507:1528	the relative abundance of several taxa	1507:1544	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	2	30	theme	subcritical	381:391	arg1	pectin					417:422	subcritical water-hydrolyzed citrus pectin	381:422	subcritical water-hydrolyzed citrus pectin (SCP)	381:428	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	2	30	theme	subcritical	381:391	arg1	SCP					425:427	SCP	425:427	SCP	425:427	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	8	31	theme	cytokines	1295:1303	arg1	expression					1266:1275	the expression	1262:1275	the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α)	1262:1334	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	9	32	theme	16S	1356:1358	arg1	sequencing					1365:1374	the 16S rDNA sequencing	1352:1374	the 16S rDNA sequencing analysis on an IlluminaMiSeq platform	1352:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	9	33	from	analysis	1376:1383	arg1	platform					1405:1412	an IlluminaMiSeq platform	1388:1412	an IlluminaMiSeq platform	1388:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	2	34	theme	immune	437:442	arg1	response					444:451	the immune response	433:451	the immune response	433:451	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	0	35	theme	Water-Hydrolyzed	27:42	arg1	Pectin					51:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	11	36	theme	study	1772:1776	arg1	results					1756:1762	The results	1752:1762	The results of this study	1752:1776	The results of this study suggest an innovative application of citrus pectin as an immunomodulator.					
32178470	10	37	theme	several	1533:1539	arg1	taxa					1541:1544	several taxa	1533:1544	several taxa	1533:1544	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	0	38	theme	Subcritical	15:25	arg1	Pectin					51:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	4	39	theme	immunosuppressed	663:678	arg1	mice					684:687	immunosuppressed ICR mice	663:687	immunosuppressed ICR mice	663:687	In this research, immunosuppressed ICR mice were administrated with SCP at dosages of 300/600/1200 mg/kg.					
32178470	5	40	theme	immune	786:791	arg1	indexes					799:805	immune organ indexes	786:805	immune organ indexes	786:805	bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined.					
32178470	2	41	theme	possible	361:368	arg1	effects					370:376	the possible effects	357:376	the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response	357:451	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	11	42	theme	pectin	1822:1827	arg1	application					1800:1810	an innovative application	1786:1810	an innovative application of citrus pectin as an immunomodulator	1786:1849	The results of this study suggest an innovative application of citrus pectin as an immunomodulator.					
32178470	3	43	theme	intestinal	533:542	arg1	dysbiosis					554:562	intestinal microbial dysbiosis	533:562	intestinal microbial dysbiosis	533:562	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	7	44	with	treatment	1029:1037	arg1	SCP					1044:1046	SCP	1044:1046	SCP	1044:1046	Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin.					
32178470	5	45	theme	organ	793:797	arg1	indexes					799:805	immune organ indexes	786:805	immune organ indexes	786:805	bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined.					
32178470	11	46	theme	citrus	1815:1820	arg1	pectin					1822:1827	citrus pectin	1815:1827	citrus pectin	1815:1827	The results of this study suggest an innovative application of citrus pectin as an immunomodulator.					
32178470	3	47	theme	immunomodulatory	502:517	arg1	functions					519:527	immunomodulatory functions	502:527	immunomodulatory functions	502:527	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	0	48	theme	Citrus	44:49	arg1	Pectin					51:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	9	49	theme	Cy-induced	1441:1450	arg1	dysbiosis					1456:1464	Cy-induced gut dysbiosis	1441:1464	Cy-induced gut dysbiosis	1441:1464	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	6	50	from	content	954:960	arg1	hydrolysates					1000:1011	citrus pectin hydrolysates	986:1011	citrus pectin hydrolysates	986:1011	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	11	51	theme	innovative	1789:1798	arg1	application					1800:1810	an innovative application	1786:1810	an innovative application of citrus pectin as an immunomodulator	1786:1849	The results of this study suggest an innovative application of citrus pectin as an immunomodulator.					
32178470	2	52	theme	Pectic	289:294	arg1	heteropolysaccharide					296:315	Pectic heteropolysaccharide	289:315	Pectic heteropolysaccharide	289:315	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	7	53	theme	original	1123:1130	arg1	pectin					1139:1144	the original citrus pectin	1119:1144	the original citrus pectin	1119:1144	Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin.					
32178470	1	54	theme	functional	269:278	arg1	regions					280:286	its functional regions	265:286	its functional regions	265:286	Subcritical water can effectively hydrolyze pectin into smaller molecules while still maintaining its functional regions.					
32178470	0	55	theme	Cyclophosphamide-Induced	70:93	arg1	Immunosuppression					95:111	Cyclophosphamide-Induced Immunosuppression	70:111	Cyclophosphamide-Induced Immunosuppression	70:111	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	9	56	theme	gut	1452:1454	arg1	dysbiosis					1456:1464	Cy-induced gut dysbiosis	1441:1464	Cy-induced gut dysbiosis	1441:1464	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	8	57	theme	immune	1213:1218	arg1	indexes					1226:1232	immune organ indexes	1213:1232	immune organ indexes	1213:1232	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	7	58	theme	citrus	1132:1137	arg1	pectin					1139:1144	the original citrus pectin	1119:1144	the original citrus pectin	1119:1144	Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin.					
32178470	8	59	theme	accelerated	1189:1199	arg1	recovery					1201:1208	accelerated recovery	1189:1208	accelerated recovery of immune organ indexes	1189:1232	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	10	60	theme	Cy-dependent	1483:1494	arg1	changes					1496:1502	Cy-dependent changes	1483:1502	Cy-dependent changes in the relative abundance of several taxa	1483:1544	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	3	61	theme	microbial	544:552	arg1	dysbiosis					554:562	intestinal microbial dysbiosis	533:562	intestinal microbial dysbiosis	533:562	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	2	62	theme	pectin	417:422	arg1	effects					370:376	the possible effects	357:376	the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response	357:451	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	9	63	theme	IlluminaMiSeq	1391:1403	arg1	platform					1405:1412	an IlluminaMiSeq platform	1388:1412	an IlluminaMiSeq platform	1388:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	5	64	theme	body	773:776	arg1	weight					778:783	body weight	773:783	body weight	773:783	bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined.					
32178470	6	65	theme	molecular	921:929	arg1	mass					931:934	the molecular mass	917:934	the molecular mass	917:934	The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates.					
32178470	8	66	theme	indexes	1226:1232	arg1	recovery					1201:1208	accelerated recovery	1189:1208	accelerated recovery of immune organ indexes	1189:1232	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	0	67	with	Treatment	0:8	arg1	Pectin					51:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Subcritical Water-Hydrolyzed Citrus Pectin	15:56	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	1	68	theme	Subcritical	167:177	arg1	water					179:183	Subcritical water	167:183	Subcritical water	167:183	Subcritical water can effectively hydrolyze pectin into smaller molecules while still maintaining its functional regions.					
32178470	8	69	theme	organ	1220:1224	arg1	indexes					1226:1232	immune organ indexes	1213:1232	immune organ indexes	1213:1232	For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α).					
32178470	0	70	from	Composition	142:152	arg1	Mice					161:164	ICR Mice	157:164	ICR Mice	157:164	Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice.					
32178470	2	71	theme	immune	329:334	arg1	regulation					336:345	immune regulation	329:345	immune regulation	329:345	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	3	72	from	effects	484:490	arg1	functions					519:527	immunomodulatory functions	502:527	immunomodulatory functions	502:527	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	3	72	from	effects	484:490	arg1	dysbiosis					554:562	intestinal microbial dysbiosis	533:562	intestinal microbial dysbiosis	533:562	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	2	73	theme	water-hydrolyzed	393:408	arg1	pectin					417:422	subcritical water-hydrolyzed citrus pectin	381:422	subcritical water-hydrolyzed citrus pectin (SCP)	381:428	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	2	73	theme	water-hydrolyzed	393:408	arg1	SCP					425:427	SCP	425:427	SCP	425:427	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	2	74	theme	citrus	410:415	arg1	pectin					417:422	subcritical water-hydrolyzed citrus pectin	381:422	subcritical water-hydrolyzed citrus pectin (SCP)	381:428	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	2	74	theme	citrus	410:415	arg1	SCP					425:427	SCP	425:427	SCP	425:427	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
32178470	5	75	theme	gut	823:825	arg1	microbiota					827:836	gut microbiota	823:836	gut microbiota	823:836	bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined.					
32178470	4	76	theme	mg/kg	744:748	arg1	dosages					720:726	dosages	720:726	dosages of 300/600/1200 mg/kg	720:748	In this research, immunosuppressed ICR mice were administrated with SCP at dosages of 300/600/1200 mg/kg.					
32178470	10	77	from	changes	1496:1502	arg1	abundance					1520:1528	the relative abundance	1507:1528	the relative abundance of several taxa	1507:1544	SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus).					
32178470	3	78	theme	cyclophosphamide-induced	590:613	arg1	model					638:642	a cyclophosphamide-induced immunosuppressed mouse model	588:642	a cyclophosphamide-induced immunosuppressed mouse model	588:642	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	9	79	theme	sequencing	1365:1374	arg1	analysis					1376:1383	the 16S rDNA sequencing analysis	1352:1383	the 16S rDNA sequencing analysis on an IlluminaMiSeq platform	1352:1412	The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis.					
32178470	7	80	theme	functional	1074:1083	arg1	properties					1085:1094	immunoregulatory functional properties	1057:1094	immunoregulatory functional properties	1057:1094	Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin.					
32178470	3	81	theme	immunosuppressed	615:630	arg1	model					638:642	a cyclophosphamide-induced immunosuppressed mouse model	588:642	a cyclophosphamide-induced immunosuppressed mouse model	588:642	Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model.					
32178470	2	82	from	effects	370:376	arg1	response					444:451	the immune response	433:451	the immune response	433:451	Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear.					
33583778	8	0	theme	porous	1078:1083	arg1	structure					1085:1093	discrete porous structure	1069:1093	discrete porous structure	1069:1093	The non-loaded hydrogel showed discrete porous structure whereas loaded hydrogels were fibrous and smooth.					
33583778	1	1	theme	Metformin	144:152	arg1	purpose					108:114	The purpose	104:114	The purpose of present study	104:131	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	1	1	theme	Metformin	144:152	arg1	HCl					154:156	load Metformin HCl	139:156	load Metformin HCl into pH-sensitive hydrogels	139:184	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	6	2	theme	bonding	876:882	arg1	Formation					856:864	Formation	856:864	Formation of strong bonding between pectin and AA	856:904	Formation of strong bonding between pectin and AA was supported by FTIR.					
33583778	10	3	theme	concentrations	1335:1348	arg1	increase					1309:1316	the increase	1305:1316	the increase of AA and pectin concentrations	1305:1348	The diffusion coefficient decreases with the increase of AA and pectin concentrations.					
33583778	3	4	theme	dynamic/equilibrium	587:605	arg1	swelling					607:614	dynamic/equilibrium swelling	587:614	dynamic/equilibrium swelling	587:614	Hydrogels were characterized for dynamic/equilibrium swelling, sol-gel fraction analysis, diffusion coefficient and percentage porosity.					
33583778	3	5	theme	diffusion	644:652	arg1	coefficient					654:664	diffusion coefficient	644:664	diffusion coefficient	644:664	Hydrogels were characterized for dynamic/equilibrium swelling, sol-gel fraction analysis, diffusion coefficient and percentage porosity.					
33583778	10	6	theme	pectin	1328:1333	arg1	concentrations					1335:1348	pectin concentrations	1328:1348	pectin concentrations	1328:1348	The diffusion coefficient decreases with the increase of AA and pectin concentrations.					
33583778	2	7	theme	occurring	279:287	arg1	pectin					304:309	naturally occurring polysaccharide pectin	269:309	naturally occurring polysaccharide pectin	269:309	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	9	8	theme	pH	1248:1249	arg1	solutions					1235:1243	the solutions	1231:1243	the solutions of pH 1.2 and 5.5	1231:1261	Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5.					
33583778	0	9	theme	metformin	93:101	arg1	delivery					81:88	controlled delivery	70:88	controlled delivery of metformin	70:101	Cross-linked pH-sensitive pectin and acrylic acid based hydrogels for controlled delivery of metformin.					
33583778	9	10	theme	higher	1162:1167	arg1	swelling					1169:1176	higher swelling	1162:1176	higher swelling in the solutions of pH 6.5 and 7.5	1162:1211	Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5.					
33583778	2	11	from	45°C	452:455	arg1	polymerization					434:447	polymerization	434:447	polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs	434:551	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	5	12	theme	drug	807:810	arg1	data					820:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data was applied to kinetic models.					
33583778	0	13	link	Cross-linked	0:11	arg1	pectin					26:31	Cross-linked pH-sensitive pectin	0:31	Cross-linked pH-sensitive pectin	0:31	Cross-linked pH-sensitive pectin and acrylic acid based hydrogels for controlled delivery of metformin.					
33583778	11	14	theme	EGDMA	1387:1391	arg1	porosity					1407:1414	the EGDMA concentration porosity	1383:1414	the EGDMA concentration porosity decreases	1383:1424	It was observed upon increasing the EGDMA concentration porosity decreases.					
33583778	3	15	theme	percentage	670:679	arg1	porosity					681:688	percentage porosity	670:688	percentage porosity	670:688	Hydrogels were characterized for dynamic/equilibrium swelling, sol-gel fraction analysis, diffusion coefficient and percentage porosity.					
33583778	7	16	theme	peaks	950:954	arg1	intensity					933:941	The intensity	929:941	The intensity of XRD peaks	929:954	The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance.					
33583778	1	17	theme	pH-sensitive	163:174	arg1	hydrogels					176:184	pH-sensitive hydrogels	163:184	pH-sensitive hydrogels	163:184	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	0	18	theme	pH-sensitive	13:24	arg1	pectin					26:31	Cross-linked pH-sensitive pectin	0:31	Cross-linked pH-sensitive pectin	0:31	Cross-linked pH-sensitive pectin and acrylic acid based hydrogels for controlled delivery of metformin.					
33583778	8	19	theme	loaded	1103:1108	arg1	hydrogels					1110:1118	loaded hydrogels	1103:1118	loaded hydrogels	1103:1118	The non-loaded hydrogel showed discrete porous structure whereas loaded hydrogels were fibrous and smooth.					
33583778	2	20	theme	monomer	315:321	arg1	AA					337:338	AA	337:338	AA	337:338	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	20	theme	monomer	315:321	arg1	acid					331:334	monomer acrylic acid	315:334	monomer acrylic acid (AA)	315:339	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	0	21	theme	Cross-linked	0:11	arg1	pectin					26:31	Cross-linked pH-sensitive pectin	0:31	Cross-linked pH-sensitive pectin	0:31	Cross-linked pH-sensitive pectin and acrylic acid based hydrogels for controlled delivery of metformin.					
33583778	2	22	from	65˚C	537:540	arg1	polymerization					434:447	polymerization	434:447	polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs	434:551	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	23	from	55°C	487:490	arg1	polymerization					434:447	polymerization	434:447	polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs	434:551	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	5	24	theme	kinetic	840:846	arg1	models					848:853	kinetic models	840:853	kinetic models	840:853	Experimental in-vitro drug release data was applied to kinetic models.					
33583778	7	25	theme	drug	1022:1025	arg1	substance					1027:1035	active drug substance	1015:1035	active drug substance	1015:1035	The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance.					
33583778	10	26	theme	AA	1321:1322	arg1	increase					1309:1316	the increase	1305:1316	the increase of AA and pectin concentrations	1305:1348	The diffusion coefficient decreases with the increase of AA and pectin concentrations.					
33583778	2	27	theme	controlled	408:417	arg1	conditions					419:428	controlled conditions	408:428	controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs	408:551	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	5	28	theme	Experimental	785:796	arg1	data					820:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data was applied to kinetic models.					
33583778	12	29	theme	hydrogels	1472:1480	arg1	compositions					1456:1467	all compositions	1452:1467	all compositions of hydrogels	1452:1480	The release of drug from all compositions of hydrogels took place through non-Fickian diffusion mechanism.					
33583778	2	30	from	50°C	469:472	arg1	polymerization					434:447	polymerization	434:447	polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs	434:551	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	31	theme	polysaccharide	289:302	arg1	pectin					304:309	naturally occurring polysaccharide pectin	269:309	naturally occurring polysaccharide pectin	269:309	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	11	32	theme	concentration	1393:1405	arg1	porosity					1407:1414	the EGDMA concentration porosity	1383:1414	the EGDMA concentration porosity decreases	1383:1424	It was observed upon increasing the EGDMA concentration porosity decreases.					
33583778	1	33	theme	sustained	194:202	arg1	release					204:210	sustained release	194:210	sustained release	194:210	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	3	34	theme	sol-gel	617:623	arg1	analysis					634:641	sol-gel fraction analysis	617:641	sol-gel fraction analysis	617:641	Hydrogels were characterized for dynamic/equilibrium swelling, sol-gel fraction analysis, diffusion coefficient and percentage porosity.					
33583778	5	35	theme	release	812:818	arg1	data					820:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data was applied to kinetic models.					
33583778	0	36	theme	acrylic	37:43	arg1	acid					45:48	acrylic acid	37:48	acrylic acid	37:48	Cross-linked pH-sensitive pectin and acrylic acid based hydrogels for controlled delivery of metformin.					
33583778	7	37	theme	active	1015:1020	arg1	substance					1027:1035	active drug substance	1015:1035	active drug substance	1015:1035	The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance.					
33583778	8	38	theme	discrete	1069:1076	arg1	structure					1085:1093	discrete porous structure	1069:1093	discrete porous structure	1069:1093	The non-loaded hydrogel showed discrete porous structure whereas loaded hydrogels were fibrous and smooth.					
33583778	9	39	theme	7.5	1209:1211	arg1	solutions					1185:1193	the solutions	1181:1193	the solutions of pH 6.5 and 7.5	1181:1211	Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5.					
33583778	7	40	theme	non-loaded	971:980	arg1	hydrogels					993:1001	non-loaded and loaded hydrogels	971:1001	non-loaded and loaded hydrogels	971:1001	The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance.					
33583778	6	41	theme	strong	869:874	arg1	bonding					876:882	strong bonding	869:882	strong bonding	869:882	Formation of strong bonding between pectin and AA was supported by FTIR.					
33583778	3	42	theme	fraction	625:632	arg1	analysis					634:641	sol-gel fraction analysis	617:641	sol-gel fraction analysis	617:641	Hydrogels were characterized for dynamic/equilibrium swelling, sol-gel fraction analysis, diffusion coefficient and percentage porosity.					
33583778	1	43	theme	load	139:142	arg1	purpose					108:114	The purpose	104:114	The purpose of present study	104:131	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	1	43	theme	load	139:142	arg1	HCl					154:156	load Metformin HCl	139:156	load Metformin HCl into pH-sensitive hydrogels	139:184	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	10	44	theme	diffusion	1268:1276	arg1	coefficient					1278:1288	The diffusion coefficient	1264:1288	The diffusion coefficient	1264:1288	The diffusion coefficient decreases with the increase of AA and pectin concentrations.					
33583778	1	45	theme	present	119:125	arg1	study					127:131	present study	119:131	present study	119:131	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	9	46	theme	5.5	1259:1261	arg1	solutions					1235:1243	the solutions	1231:1243	the solutions of pH 1.2 and 5.5	1231:1261	Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5.					
33583778	9	47	from	swelling	1169:1176	arg1	solutions					1185:1193	the solutions	1181:1193	the solutions of pH 6.5 and 7.5	1181:1211	Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5.					
33583778	1	48	theme	study	127:131	arg1	HCl					154:156	load Metformin HCl	139:156	load Metformin HCl into pH-sensitive hydrogels	139:184	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	1	48	theme	study	127:131	arg1	purpose					108:114	The purpose	104:114	The purpose of present study	104:131	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	12	49	theme	diffusion	1513:1521	arg1	mechanism					1523:1531	non-Fickian diffusion mechanism	1501:1531	non-Fickian diffusion mechanism	1501:1531	The release of drug from all compositions of hydrogels took place through non-Fickian diffusion mechanism.					
33583778	7	50	theme	loaded	986:991	arg1	hydrogels					993:1001	non-loaded and loaded hydrogels	971:1001	non-loaded and loaded hydrogels	971:1001	The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance.					
33583778	12	51	theme	drug	1442:1445	arg1	release					1431:1437	The release	1427:1437	The release of drug from all compositions of hydrogels	1427:1480	The release of drug from all compositions of hydrogels took place through non-Fickian diffusion mechanism.					
33583778	12	52	from	compositions	1456:1467	arg1	release					1431:1437	The release	1427:1437	The release of drug from all compositions of hydrogels	1427:1480	The release of drug from all compositions of hydrogels took place through non-Fickian diffusion mechanism.					
33583778	7	53	theme	XRD	946:948	arg1	peaks					950:954	XRD peaks	946:954	XRD peaks	946:954	The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance.					
33583778	2	54	theme	acrylic	323:329	arg1	AA					337:338	AA	337:338	AA	337:338	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	54	theme	acrylic	323:329	arg1	acid					331:334	monomer acrylic acid	315:334	monomer acrylic acid (AA)	315:339	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	12	55	theme	non-Fickian	1501:1511	arg1	mechanism					1523:1531	non-Fickian diffusion mechanism	1501:1531	non-Fickian diffusion mechanism	1501:1531	The release of drug from all compositions of hydrogels took place through non-Fickian diffusion mechanism.					
33583778	0	56	theme	controlled	70:79	arg1	delivery					81:88	controlled delivery	70:88	controlled delivery of metformin	70:101	Cross-linked pH-sensitive pectin and acrylic acid based hydrogels for controlled delivery of metformin.					
33583778	1	57	contain	have	189:192	arg2	release					204:210	sustained release	194:210	sustained release	194:210	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	1	57	contain	have	189:192	arg1	HCl					154:156	load Metformin HCl	139:156	load Metformin HCl into pH-sensitive hydrogels	139:184	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	1	57	contain	have	189:192	arg1	purpose					108:114	The purpose	104:114	The purpose of present study	104:131	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
33583778	4	58	theme	surface	752:758	arg1	morphology					760:769	surface morphology	752:769	surface morphology	752:769	Hydrogels were tested by FTIR, XRD and SEM for structure and surface morphology respectively.					
33583778	5	59	theme	in-vitro	798:805	arg1	data					820:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data	785:823	Experimental in-vitro drug release data was applied to kinetic models.					
33583778	2	60	theme	glycol	356:361	arg1	EGDMA					379:383	EGDMA	379:383	EGDMA	379:383	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	60	theme	glycol	356:361	arg1	dimethacrylate					363:376	ethylene glycol dimethacrylate	347:376	ethylene glycol dimethacrylate (EGDMA)	347:384	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	61	from	60°C	507:510	arg1	polymerization					434:447	polymerization	434:447	polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs	434:551	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	9	62	theme	pH	1198:1199	arg1	solutions					1185:1193	the solutions	1181:1193	the solutions of pH 6.5 and 7.5	1181:1211	Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5.					
33583778	11	63	dep	porosity	1407:1414	arg1	decreases					1416:1424	decreases	1416:1424	decreases	1416:1424	It was observed upon increasing the EGDMA concentration porosity decreases.					
33583778	2	64	theme	ethylene	347:354	arg1	EGDMA					379:383	EGDMA	379:383	EGDMA	379:383	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	2	64	theme	ethylene	347:354	arg1	dimethacrylate					363:376	ethylene glycol dimethacrylate	347:376	ethylene glycol dimethacrylate (EGDMA)	347:384	The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs.					
33583778	8	65	theme	non-loaded	1042:1051	arg1	hydrogel					1053:1060	The non-loaded hydrogel	1038:1060	The non-loaded hydrogel	1038:1060	The non-loaded hydrogel showed discrete porous structure whereas loaded hydrogels were fibrous and smooth.					
33583778	1	66	theme	time	229:232	arg1	period					219:224	a period	217:224	a period of time	217:232	The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time.					
32684519	2	0	theme	adsorbent	497:505	arg1	dosing					507:512	adsorbent dosing	497:512	adsorbent dosing	497:512	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	3	1	theme	chemical	738:745	arg1	process					747:753	the chemical process	734:753	the chemical process dominated by the molecular layer	734:786	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	9	2	theme	solution	1603:1610	arg1	sample					1612:1617	the adsorbed solution sample	1590:1617	the adsorbed solution sample	1590:1617	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	6	3	theme	excellent	1273:1281	arg1	responsiveness					1292:1305	excellent magnetic responsiveness	1273:1305	excellent magnetic responsiveness	1273:1305	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	3	4	dep	suggested	694:702	arg1	limited					723:729	limited	723:729	suggested the adsorption was limited by the chemical process dominated by the molecular layer	694:786	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	2	5	theme	adsorption	557:566	arg1	performance					568:578	adsorption performance	557:578	adsorption performance	557:578	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	1	6	theme	toxic	345:349	arg1	L-cysteine					371:380	L-cysteine	371:380	L-cysteine	371:380	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	1	6	theme	toxic	345:349	arg1	alginate					393:400	sodium alginate	386:400	sodium alginate	386:400	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	1	6	theme	toxic	345:349	arg1	materials					351:359	two non-biologically toxic materials	324:359	two non-biologically toxic materials including L-cysteine and sodium alginate	324:400	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	7	7	theme	amino	1412:1416	arg1	ions					1437:1440	amino, carboxyl and lead ions	1412:1440	amino, carboxyl and lead ions	1412:1440	It also has been found that the adsorption mechanism mainly included ion exchange and chelation between amino, carboxyl and lead ions.					
32684519	2	8	theme	contact	541:547	arg1	time					549:552	contact time	541:552	contact time	541:552	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	2	9	from	influences	453:462	arg1	performance					568:578	adsorption performance	557:578	adsorption performance	557:578	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	6	10	theme	adsorption	1252:1261	arg1	sites					1263:1267	sufficient adsorption sites	1241:1267	sufficient adsorption sites	1241:1267	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	6	11	theme	contact	1203:1209	arg1	area					1211:1214	a large contact area	1195:1214	a large contact area	1195:1214	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	4	12	theme	Langmuir	798:805	arg1	model					816:820	Langmuir isotherm model	798:820	Langmuir isotherm model	798:820	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	1	13	theme	lead	265:268	arg1	ions					270:273	lead ions	265:273	lead ions in the solution	265:289	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	6	14	theme	sufficient	1241:1250	arg1	sites					1263:1267	sufficient adsorption sites	1241:1267	sufficient adsorption sites	1241:1267	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	9	15	theme	iron	1563:1566	arg1	0.34 mg/L					1656:1664	0.34 mg/L	1656:1664	0.34 mg/L	1656:1664	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	9	15	theme	iron	1563:1566	arg1	concentration					1573:1585	The average iron ions concentration	1551:1585	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution	1551:1649	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	8	16	theme	98.04	1498:1502	arg1	%					1503:1503	%	1503:1503	%	1503:1503	After 5 cycles, the adsorption capacity decreased from 98.04% to 87.40% and still maintained at high level.					
32684519	5	17	theme	Various	944:950	arg1	VSM					995:997	VSM	995:997	VSM	995:997	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	17	theme	Various	944:950	arg1	potential					1018:1026	Zeta potential	1013:1026	Zeta potential	1013:1026	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	17	theme	Various	944:950	arg1	SEM-EDS					980:986	SEM-EDS	980:986	SEM-EDS	980:986	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	17	theme	Various	944:950	arg1	TGA					1000:1002	TGA	1000:1002	TGA	1000:1002	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	17	theme	Various	944:950	arg1	XPS					1005:1007	XPS	1005:1007	XPS	1005:1007	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	17	theme	Various	944:950	arg1	methods					963:969	Various analytical methods	944:969	Various analytical methods	944:969	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	17	theme	Various	944:950	arg1	FTIR					989:992	FTIR	989:992	FTIR	989:992	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	6	18	theme	Characterization	1139:1154	arg1	results					1156:1162	Characterization results	1139:1162	Characterization results	1139:1162	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	1	19	theme	environmental	161:173	arg1	MSAL					214:217	environmental friendly magnetic composite adsorbent (MSAL)	161:218	environmental friendly magnetic composite adsorbent (MSAL)	161:218	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	2	20	theme	different	467:475	arg1	parameters					477:486	different parameters	467:486	different parameters like pH, adsorbent dosing, initial concentration and contact time	467:552	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	7	21	theme	lead	1432:1435	arg1	ions					1437:1440	amino, carboxyl and lead ions	1412:1440	amino, carboxyl and lead ions	1412:1440	It also has been found that the adsorption mechanism mainly included ion exchange and chelation between amino, carboxyl and lead ions.					
32684519	9	22	theme	regeneration	1629:1640	arg1	solution					1642:1649	the regeneration solution	1625:1649	the regeneration solution	1625:1649	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	4	23	theme	isotherm	807:814	arg1	model					816:820	Langmuir isotherm model	798:820	Langmuir isotherm model	798:820	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	9	24	from	concentration	1573:1585	arg1	solution					1642:1649	the regeneration solution	1625:1649	the regeneration solution	1625:1649	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	9	24	from	concentration	1573:1585	arg1	sample					1612:1617	the adsorbed solution sample	1590:1617	the adsorbed solution sample	1590:1617	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	1	25	theme	friendly	175:182	arg1	MSAL					214:217	environmental friendly magnetic composite adsorbent (MSAL)	161:218	environmental friendly magnetic composite adsorbent (MSAL)	161:218	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	0	26	theme	environmental-friendly	3:24	arg1	bio-adsorbent					35:47	An environmental-friendly magnetic bio-adsorbent	0:47	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.	0:144	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	1	27	theme	sodium	386:391	arg1	alginate					393:400	sodium alginate	386:400	sodium alginate	386:400	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	6	28	theme	thermal	1222:1228	arg1	stability					1230:1238	good thermal stability	1217:1238	good thermal stability	1217:1238	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	8	29	dep	%	1503:1503	arg1	to					1505:1506	to	1505:1506	to	1505:1506	After 5 cycles, the adsorption capacity decreased from 98.04% to 87.40% and still maintained at high level.					
32684519	1	30	theme	magnetic	184:191	arg1	MSAL					214:217	environmental friendly magnetic composite adsorbent (MSAL)	161:218	environmental friendly magnetic composite adsorbent (MSAL)	161:218	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	7	31	theme	carboxyl	1419:1426	arg1	ions					1437:1440	amino, carboxyl and lead ions	1412:1440	amino, carboxyl and lead ions	1412:1440	It also has been found that the adsorption mechanism mainly included ion exchange and chelation between amino, carboxyl and lead ions.					
32684519	6	32	theme	good	1217:1220	arg1	stability					1230:1238	good thermal stability	1217:1238	good thermal stability	1217:1238	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	3	33	theme	sorption	596:603	arg1	process					605:611	sorption process	596:611	sorption process	596:611	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	1	34	theme	composite	193:201	arg1	MSAL					214:217	environmental friendly magnetic composite adsorbent (MSAL)	161:218	environmental friendly magnetic composite adsorbent (MSAL)	161:218	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	4	35	theme	Pb	835:836	arg1	capacity					852:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	35	theme	Pb	835:836	arg1	330 mg/g					871:878	330 mg/g	871:878	330 mg/g	871:878	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	0	36	theme	adsorption	122:131	arg1	performance					133:143	its adsorption performance	118:143	its adsorption performance	118:143	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	9	37	theme	ions	1568:1571	arg1	0.34 mg/L					1656:1664	0.34 mg/L	1656:1664	0.34 mg/L	1656:1664	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	9	37	theme	ions	1568:1571	arg1	concentration					1573:1585	The average iron ions concentration	1551:1585	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution	1551:1649	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	5	38	theme	analytical	952:961	arg1	VSM					995:997	VSM	995:997	VSM	995:997	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	38	theme	analytical	952:961	arg1	potential					1018:1026	Zeta potential	1013:1026	Zeta potential	1013:1026	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	38	theme	analytical	952:961	arg1	SEM-EDS					980:986	SEM-EDS	980:986	SEM-EDS	980:986	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	38	theme	analytical	952:961	arg1	TGA					1000:1002	TGA	1000:1002	TGA	1000:1002	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	38	theme	analytical	952:961	arg1	XPS					1005:1007	XPS	1005:1007	XPS	1005:1007	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	38	theme	analytical	952:961	arg1	methods					963:969	Various analytical methods	944:969	Various analytical methods	944:969	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	5	38	theme	analytical	952:961	arg1	FTIR					989:992	FTIR	989:992	FTIR	989:992	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	1	39	theme	adsorbent	203:211	arg1	MSAL					214:217	environmental friendly magnetic composite adsorbent (MSAL)	161:218	environmental friendly magnetic composite adsorbent (MSAL)	161:218	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	0	40	theme	magnetic	26:33	arg1	bio-adsorbent					35:47	An environmental-friendly magnetic bio-adsorbent	0:47	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.	0:144	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	3	41	theme	molecular	772:780	arg1	layer					782:786	the molecular layer	768:786	the molecular layer	768:786	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	1	42	from	ions	270:273	arg1	solution					282:289	the solution	278:289	the solution	278:289	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	7	43	theme	adsorption	1340:1349	arg1	mechanism					1351:1359	the adsorption mechanism	1336:1359	the adsorption mechanism	1336:1359	It also has been found that the adsorption mechanism mainly included ion exchange and chelation between amino, carboxyl and lead ions.					
32684519	0	44	theme	high-efficiency	53:67	arg1	removal					75:81	high-efficiency Pb(Ⅱ) removal	53:81	high-efficiency Pb(Ⅱ) removal	53:81	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	4	45	theme	adsorbents	932:941	arg1	amount					911:916	a large amount	903:916	a large amount of other lead adsorbents	903:941	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	45	theme	adsorbents	932:941	arg1	adsorbents					932:941	other lead adsorbents	921:941	other lead adsorbents	921:941	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	46	theme	Ⅱ	838:838	arg1	capacity					852:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	46	theme	Ⅱ	838:838	arg1	330 mg/g					871:878	330 mg/g	871:878	330 mg/g	871:878	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	8	47	theme	high	1539:1542	arg1	level					1544:1548	high level	1539:1548	high level	1539:1548	After 5 cycles, the adsorption capacity decreased from 98.04% to 87.40% and still maintained at high level.					
32684519	4	48	theme	lead	927:930	arg1	adsorbents					932:941	other lead adsorbents	921:941	other lead adsorbents	921:941	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	3	49	theme	isotherm	672:679	arg1	model					681:685	Langmuir isotherm model	663:685	Langmuir isotherm model	663:685	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	3	50	theme	pseudo-second-order	625:643	arg1	model					653:657	pseudo-second-order kinetic model	625:657	pseudo-second-order kinetic model	625:657	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	2	51	theme	initial	515:521	arg1	concentration					523:535	initial concentration	515:535	initial concentration	515:535	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	2	52	theme	parameters	477:486	arg1	influences					453:462	the influences	449:462	the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance	449:578	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	9	53	theme	average	1555:1561	arg1	0.34 mg/L					1656:1664	0.34 mg/L	1656:1664	0.34 mg/L	1656:1664	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	9	53	theme	average	1555:1561	arg1	concentration					1573:1585	The average iron ions concentration	1551:1585	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution	1551:1649	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	4	54	theme	adsorption	841:850	arg1	capacity					852:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	54	theme	adsorption	841:850	arg1	330 mg/g					871:878	330 mg/g	871:878	330 mg/g	871:878	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	8	55	theme	87.40	1508:1512	arg1	%					1503:1503	%	1503:1503	%	1503:1503	After 5 cycles, the adsorption capacity decreased from 98.04% to 87.40% and still maintained at high level.					
32684519	0	56	theme	Pb	69:70	arg1	removal					75:81	high-efficiency Pb(Ⅱ) removal	53:81	high-efficiency Pb(Ⅱ) removal	53:81	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	5	57	theme	adsorption	1117:1126	arg1	mechanism					1128:1136	the adsorption mechanism	1113:1136	the adsorption mechanism	1113:1136	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	4	58	theme	other	921:925	arg1	adsorbents					932:941	other lead adsorbents	921:941	other lead adsorbents	921:941	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	6	59	theme	large	1197:1201	arg1	area					1211:1214	a large contact area	1195:1214	a large contact area	1195:1214	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	9	60	theme	adsorbed	1594:1601	arg1	sample					1612:1617	the adsorbed solution sample	1590:1617	the adsorbed solution sample	1590:1617	The average iron ions concentration in the adsorbed solution sample or in the regeneration solution were 0.34 mg/L and 0.15 mg/L.					
32684519	6	61	theme	magnetic	1283:1290	arg1	responsiveness					1292:1305	excellent magnetic responsiveness	1273:1305	excellent magnetic responsiveness	1273:1305	Characterization results found this adsorbent exhibited a large contact area, good thermal stability, sufficient adsorption sites and excellent magnetic responsiveness.					
32684519	5	62	theme	Zeta	1013:1016	arg1	potential					1018:1026	Zeta potential	1013:1026	Zeta potential	1013:1026	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	0	63	dep	bio-adsorbent	35:47	arg1	performance					133:143	its adsorption performance	118:143	its adsorption performance	118:143	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	0	63	dep	bio-adsorbent	35:47	arg1	characterization					97:112	characterization	97:112	characterization	97:112	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	0	63	dep	bio-adsorbent	35:47	arg1	Preparation					84:94	Preparation	84:94	Preparation	84:94	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	7	64	theme	ion	1377:1379	arg1	exchange					1381:1388	ion exchange	1377:1388	ion exchange	1377:1388	It also has been found that the adsorption mechanism mainly included ion exchange and chelation between amino, carboxyl and lead ions.					
32684519	4	65	theme	large	905:909	arg1	amount					911:916	a large amount	903:916	a large amount of other lead adsorbents	903:941	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	65	theme	large	905:909	arg1	adsorbents					932:941	other lead adsorbents	921:941	other lead adsorbents	921:941	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	3	66	theme	Langmuir	663:670	arg1	model					681:685	Langmuir isotherm model	663:685	Langmuir isotherm model	663:685	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	8	67	theme	adsorption	1463:1472	arg1	capacity					1474:1481	the adsorption capacity	1459:1481	the adsorption capacity	1459:1481	After 5 cycles, the adsorption capacity decreased from 98.04% to 87.40% and still maintained at high level.					
32684519	3	68	theme	kinetic	645:651	arg1	model					653:657	pseudo-second-order kinetic model	625:657	pseudo-second-order kinetic model	625:657	Results showed sorption process followed by pseudo-second-order kinetic model and Langmuir isotherm model, which suggested the adsorption was limited by the chemical process dominated by the molecular layer.					
32684519	10	69	theme	above	1694:1698	arg1	results					1700:1706	all above results	1690:1706	all above results	1690:1706	Overall, all above results imply that MSAL is a promising reusable adsorbent for removing Pb(Ⅱ) in solution.					
32684519	2	70	theme	Batch	403:407	arg1	experiments					409:419	Batch experiments	403:419	Batch experiments	403:419	Batch experiments were carried out to discuss the influences of different parameters like pH, adsorbent dosing, initial concentration and contact time on adsorption performance.					
32684519	1	71	theme	excellent	231:239	arg1	capacity					252:259	excellent adsorption capacity	231:259	excellent adsorption capacity for lead ions in the solution	231:289	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
32684519	0	72	theme	Ⅱ	72:72	arg1	removal					75:81	high-efficiency Pb(Ⅱ) removal	53:81	high-efficiency Pb(Ⅱ) removal	53:81	An environmental-friendly magnetic bio-adsorbent for high-efficiency Pb(Ⅱ) removal: Preparation, characterization and its adsorption performance.					
32684519	5	73	theme	adsorbent	1082:1090	arg1	performance					1062:1072	the performance	1058:1072	the performance of this adsorbent as well as exploring the adsorption mechanism	1058:1136	Various analytical methods, such as SEM-EDS, FTIR, VSM, TGA, XPS and Zeta potential, were applied to characterize the performance of this adsorbent as well as exploring the adsorption mechanism.					
32684519	4	74	theme	maximum	827:833	arg1	capacity					852:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	the maximum Pb(Ⅱ) adsorption capacity	823:859	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	4	74	theme	maximum	827:833	arg1	330 mg/g					871:878	330 mg/g	871:878	330 mg/g	871:878	Based on Langmuir isotherm model, the maximum Pb(Ⅱ) adsorption capacity was about 330 mg/g, which was better than a large amount of other lead adsorbents.					
32684519	1	75	theme	adsorption	241:250	arg1	capacity					252:259	excellent adsorption capacity	231:259	excellent adsorption capacity for lead ions in the solution	231:289	In this paper, environmental friendly magnetic composite adsorbent (MSAL), exhibited excellent adsorption capacity for lead ions in the solution, was successfully prepared using two non-biologically toxic materials including L-cysteine and sodium alginate.					
34212486	0	0	theme	micellar	87:94	arg1	chromatography					103:116	mixed micellar liquid chromatography	81:116	mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	81:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	3	1	theme	M	555:555	arg1	dodecylsulfate					564:577	0.08 M sodium dodecylsulfate	550:577	0.08 M sodium dodecylsulfate	550:577	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	0	2	theme	mixed	81:85	arg1	chromatography					103:116	mixed micellar liquid chromatography	81:116	mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	81:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	0	3	from	analysis	13:20	arg1	medicines					65:73	traditional Chinese medicines	45:73	traditional Chinese medicines	45:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	1	4	from	rich	365:368	arg1	isoflavones					373:383	isoflavones	373:383	isoflavones	373:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	8	5	from	existence	1322:1330	arg1	phase					1385:1389	the mobile phase	1374:1389	the mobile phase	1374:1389	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	3	6	theme	sodium	557:562	arg1	dodecylsulfate					564:577	0.08 M sodium dodecylsulfate	550:577	0.08 M sodium dodecylsulfate	550:577	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	8	7	theme	chromatographic	1452:1466	arg1	efficiency					1468:1477	the increased chromatographic efficiency	1438:1477	the increased chromatographic efficiency	1438:1477	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	8	8	theme	increased	1442:1450	arg1	efficiency					1468:1477	the increased chromatographic efficiency	1438:1477	the increased chromatographic efficiency	1438:1477	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	1	9	theme	active	294:299	arg1	ingredient					301:310	an active ingredient	291:310	an active ingredient for quality control of traditional Chinese medicines rich in isoflavones	291:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	0	10	theme	liquid	96:101	arg1	chromatography					103:116	mixed micellar liquid chromatography	81:116	mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	81:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	4	11	theme	column	881:886	arg1	temperature					888:898	column temperature	881:898	column temperature	881:898	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	3	12	dep	87:13	616:620	arg1	v/v					623:625	v/v	623:625	v/v	623:625	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	0	13	theme	sodium	129:134	arg1	amphiphiles					181:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	4	14	dep	type	841:844	arg1	the					837:839	the	837:839	the	837:839	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	3	15	theme	6.05	583:586	arg1	mM					588:589	mM	588:589	mM	588:589	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	8	16	theme	vesicles	1362:1369	arg1	existence					1322:1330	the existence	1318:1330	the existence of supramolecular amphiphilic vesicles in the mobile phase	1318:1389	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	1	17	from	isoflavones	373:383	arg1	rich					365:368	rich	365:368	rich	365:368	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	8	18	theme	mobile	1378:1383	arg1	phase					1385:1389	the mobile phase	1374:1389	the mobile phase	1374:1389	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	2	19	theme	micellar	488:495	arg1	chromatography					504:517	mixed micellar liquid chromatography	482:517	mixed micellar liquid chromatography	482:517	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	1	20	theme	phytoestrogens	219:232	arg1	one					208:210	one	208:210	one	208:210	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	1	20	theme	phytoestrogens	219:232	arg1	phytoestrogens					219:232	the phytoestrogens	215:232	the phytoestrogens	215:232	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	2	21	theme	liquid	497:502	arg1	chromatography					504:517	mixed micellar liquid chromatography	482:517	mixed micellar liquid chromatography	482:517	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	0	22	from	isoflavones	30:40	arg1	medicines					65:73	traditional Chinese medicines	45:73	traditional Chinese medicines	45:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	4	23	theme	solvents	867:874	arg1	ratio					850:854	ratio	850:854	ratio	850:854	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	23	theme	solvents	867:874	arg1	pH					783:784	the pH	779:784	the pH	779:784	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	23	theme	solvents	867:874	arg1	composition					791:801	composition	791:801	composition	791:801	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	23	theme	solvents	867:874	arg1	type					841:844	type	841:844	type	841:844	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	23	theme	solvents	867:874	arg1	concentration					807:819	concentration	807:819	concentration	807:819	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	23	theme	solvents	867:874	arg1	temperature					888:898	column temperature	881:898	column temperature	881:898	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	5	24	theme	Human	1033:1037	arg1	guidelines					1043:1052	Human Use guidelines	1033:1052	Human Use guidelines	1033:1052	The method was validated according to the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines.					
34212486	8	25	theme	transmission	1394:1405	arg1	microscopy					1416:1425	transmission electron microscopy to explain the increased chromatographic efficiency	1394:1477	transmission electron microscopy to explain the increased chromatographic efficiency	1394:1477	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	2	26	theme	Chinese	433:439	arg1	medicines					441:449	traditional Chinese medicines	421:449	traditional Chinese medicines	421:449	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	0	27	theme	Quantitative	0:11	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of nine isoflavones in traditional Chinese medicines	0:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	4	28	theme	organic	859:865	arg1	solvents					867:874	organic solvents	859:874	organic solvents	859:874	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	29	theme	chromatographic	742:756	arg1	temperature					888:898	column temperature	881:898	column temperature	881:898	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	29	theme	chromatographic	742:756	arg1	type					841:844	type	841:844	type	841:844	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	29	theme	chromatographic	742:756	arg1	conditions					758:767	the chromatographic conditions	738:767	the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature	738:898	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	29	theme	chromatographic	742:756	arg1	pH					783:784	the pH	779:784	the pH	779:784	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	29	theme	chromatographic	742:756	arg1	composition					791:801	composition	791:801	composition	791:801	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	2	30	theme	traditional	421:431	arg1	medicines					441:449	traditional Chinese medicines	421:449	traditional Chinese medicines	421:449	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	2	31	theme	mixed	482:486	arg1	chromatography					504:517	mixed micellar liquid chromatography	482:517	mixed micellar liquid chromatography	482:517	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	1	32	theme	quality	316:322	arg1	control					324:330	quality control	316:330	quality control of traditional Chinese medicines rich in isoflavones	316:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	0	33	theme	supramolecular	166:179	arg1	amphiphiles					181:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	8	34	theme	amphiphilic	1350:1360	arg1	vesicles					1362:1369	supramolecular amphiphilic vesicles	1335:1369	supramolecular amphiphilic vesicles	1335:1369	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	1	35	theme	estrogenic	244:253	arg1	effects					255:261	estrogenic effects	244:261	estrogenic effects	244:261	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	0	36	theme	dodecylsulfate/β-cyclodextrin	136:164	arg1	amphiphiles					181:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	1	37	contain	have	239:242	arg2	effects					255:261	estrogenic effects	244:261	estrogenic effects	244:261	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	1	37	contain	have	239:242	arg1	one					208:210	one	208:210	one	208:210	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	1	37	contain	have	239:242	arg1	phytoestrogens					219:232	the phytoestrogens	215:232	the phytoestrogens	215:232	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	8	38	theme	electron	1407:1414	arg1	microscopy					1416:1425	transmission electron microscopy to explain the increased chromatographic efficiency	1394:1477	transmission electron microscopy to explain the increased chromatographic efficiency	1394:1477	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	3	39	theme	mobile	524:529	arg1	phase					531:535	The mobile phase	520:535	The mobile phase	520:535	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	8	40	theme	supramolecular	1335:1348	arg1	vesicles					1362:1369	supramolecular amphiphilic vesicles	1335:1369	supramolecular amphiphilic vesicles	1335:1369	Finally, we verified the existence of supramolecular amphiphilic vesicles in the mobile phase by transmission electron microscopy to explain the increased chromatographic efficiency.					
34212486	3	41	from	pH	631:632	arg1	87:13					616:620	87:13	616:620	87:13	616:620	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	3	41	from	pH	631:632	arg1	methanol					606:613	methanol	606:613	methanol (87:13, v/v) at pH 3	606:634	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	0	42	theme	isoflavones	30:40	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of nine isoflavones in traditional Chinese medicines	0:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	1	43	theme	traditional	335:345	arg1	medicines					355:363	traditional Chinese medicines	335:363	traditional Chinese medicines rich in isoflavones	335:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	0	44	contain	containing	118:127	arg1	chromatography					103:116	mixed micellar liquid chromatography	81:116	mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	81:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	0	44	contain	containing	118:127	arg2	amphiphiles					181:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles	129:191	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	0	45	theme	Chinese	57:63	arg1	medicines					65:73	traditional Chinese medicines	45:73	traditional Chinese medicines	45:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	1	46	theme	Chinese	347:353	arg1	medicines					355:363	traditional Chinese medicines	335:363	traditional Chinese medicines rich in isoflavones	335:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	0	47	from	medicines	65:73	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of nine isoflavones in traditional Chinese medicines	0:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	5	48	theme	Technical	986:994	arg1	Requirements					996:1007	Technical Requirements	986:1007	Technical Requirements for Pharmaceuticals for Human Use guidelines	986:1052	The method was validated according to the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines.					
34212486	0	49	theme	traditional	45:55	arg1	medicines					65:73	traditional Chinese medicines	45:73	traditional Chinese medicines	45:73	Quantitative analysis of nine isoflavones in traditional Chinese medicines using mixed micellar liquid chromatography containing sodium dodecylsulfate/β-cyclodextrin supramolecular amphiphiles.					
34212486	1	50	theme	medicines	355:363	arg1	control					324:330	quality control	316:330	quality control of traditional Chinese medicines rich in isoflavones	316:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	6	51	theme	liquid	1089:1094	arg1	chromatography					1096:1109	micellar liquid chromatography	1080:1109	micellar liquid chromatography	1080:1109	There is no report using micellar liquid chromatography to detect isoflavones, and the optimized method has been successfully applied to quantify isoflavones in red clover and Radix Puerariae.					
34212486	3	52	theme	0.08	550:553	arg1	M					555:555	M	555:555	M	555:555	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	1	53	theme	rich	365:368	arg1	medicines					355:363	traditional Chinese medicines	335:363	traditional Chinese medicines rich in isoflavones	335:383	Isoflavone is one of the phytoestrogens that have estrogenic effects, so it is usually served as an active ingredient for quality control of traditional Chinese medicines rich in isoflavones.					
34212486	3	54	theme	mM	588:589	arg1	β-cyclodextrin					591:604	6.05 mM β-cyclodextrin	583:604	6.05 mM β-cyclodextrin	583:604	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	5	55	theme	Requirements	996:1007	arg1	Harmonization					969:981	Harmonization	969:981	Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines	969:1052	The method was validated according to the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines.					
34212486	6	56	theme	optimized	1142:1150	arg1	method					1152:1157	the optimized method	1138:1157	the optimized method	1138:1157	There is no report using micellar liquid chromatography to detect isoflavones, and the optimized method has been successfully applied to quantify isoflavones in red clover and Radix Puerariae.					
34212486	3	57	theme	C18	683:685	arg1	column					687:692	a C18 column	681:692	a C18 column	681:692	The mobile phase consisted of 0.08 M sodium dodecylsulfate and 6.05 mM β-cyclodextrin:methanol (87:13, v/v) at pH 3 and eluted isocratically at 1 mL/min through a C18 column.					
34212486	4	58	theme	surfactants	824:834	arg1	ratio					850:854	ratio	850:854	ratio	850:854	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	58	theme	surfactants	824:834	arg1	pH					783:784	the pH	779:784	the pH	779:784	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	58	theme	surfactants	824:834	arg1	composition					791:801	composition	791:801	composition	791:801	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	58	theme	surfactants	824:834	arg1	type					841:844	type	841:844	type	841:844	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	58	theme	surfactants	824:834	arg1	concentration					807:819	concentration	807:819	concentration	807:819	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	4	58	theme	surfactants	824:834	arg1	temperature					888:898	column temperature	881:898	column temperature	881:898	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	6	59	theme	micellar	1080:1087	arg1	chromatography					1096:1109	micellar liquid chromatography	1080:1109	micellar liquid chromatography	1080:1109	There is no report using micellar liquid chromatography to detect isoflavones, and the optimized method has been successfully applied to quantify isoflavones in red clover and Radix Puerariae.					
34212486	4	60	dep	composition	791:801	arg1	the					787:789	the	787:789	the	787:789	In this study, we systematically optimized the chromatographic conditions including the pH, the composition and concentration of surfactants, the type and ratio of organic solvents, and column temperature.					
34212486	2	61	located	found	412:416	arg1	medicines					441:449	traditional Chinese medicines	421:449	traditional Chinese medicines	421:449	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	2	61	located	found	412:416	arg2	isoflavones					391:401	Nine isoflavones	386:401	Nine isoflavones commonly found in traditional Chinese medicines	386:449	Nine isoflavones commonly found in traditional Chinese medicines were separated in 30 min using mixed micellar liquid chromatography.					
34212486	5	62	theme	Use	1039:1041	arg1	guidelines					1043:1052	Human Use guidelines	1033:1052	Human Use guidelines	1033:1052	The method was validated according to the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines.					
33093930	6	0	theme	structure	911:919	arg1	software					932:939	the Privateer carbohydrate structure validation software	884:939	the Privateer carbohydrate structure validation software	884:939	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	6	1	gly	glycoprotein	1055:1066	arg1	glycoprotein					1055:1066	3D glycoprotein structures	1052:1077	3D glycoprotein structures	1052:1077	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	6	2	theme	carbohydrate	898:909	arg1	software					932:939	the Privateer carbohydrate structure validation software	884:939	the Privateer carbohydrate structure validation software	884:939	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	3	3	theme	same	366:369	arg1	time					371:374	the same time	362:374	the same time	362:374	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	6	4	theme	Privateer	888:896	arg1	software					932:939	the Privateer carbohydrate structure validation software	884:939	the Privateer carbohydrate structure validation software	884:939	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	2	5	theme	prolific	286:293	arg1	microscopy					347:356	cryo-electron microscopy	333:356	cryo-electron microscopy	333:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	5	theme	prolific	286:293	arg1	techniques					295:304	the two most prolific techniques	273:304	the two most prolific techniques: X-ray crystallography and cryo-electron microscopy	273:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	5	theme	prolific	286:293	arg1	crystallography					313:327	X-ray crystallography	307:327	X-ray crystallography	307:327	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	1	6	gly	heterogeneity	86:98	arg1	glycans					128:134	glycans	128:134	glycans in glycoproteins	128:151	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	1	7	from	glycoproteins	139:151	arg1	complexity					114:123	complexity	114:123	complexity	114:123	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	1	7	from	glycoproteins	139:151	arg1	mobility					101:108	mobility	101:108	mobility	101:108	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	1	7	from	glycoproteins	139:151	arg1	heterogeneity					86:98	The heterogeneity	82:98	The heterogeneity	82:98	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	6	8	theme	glycomics	983:991	arg1	projects					993:1000	glycomics projects	983:1000	glycomics projects	983:1000	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	3	9	from	advances	377:384	arg1	spectrometry					394:405	mass spectrometry	389:405	mass spectrometry	389:405	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	6	10	from	projects	993:1000	arg1	results					970:976	results	970:976	results from glycomics projects	970:1000	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	3	11	theme	structure	518:526	arg1	methods					537:543	structure solution methods	518:543	structure solution methods	518:543	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	1	12	from	glycans	128:134	arg1	glycoproteins					139:151	glycoproteins	139:151	glycoproteins	139:151	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	3	13	theme	solution	528:535	arg1	methods					537:543	structure solution methods	518:543	structure solution methods	518:543	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	3	14	theme	deeper	436:441	arg1	insights					443:450	deeper insights	436:450	deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds	436:631	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	0	15	theme	glycomics	11:19	arg1	data					21:24	glycomics data	11:24	glycomics data	11:24	Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.					
33093930	5	16	contain	have	735:738	arg2	results					747:753	stored results	740:753	stored results	740:753	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	5	16	contain	have	735:738	arg1	initiatives					723:733	several large scale glycomics initiatives	693:733	several large scale glycomics initiatives	693:733	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	3	17	theme	glycan	562:567	arg1	composition					569:579	the full-length glycan composition	546:579	precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition	455:579	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	6	18	theme	glycoprotein	1055:1066	arg1	structures					1068:1077	3D glycoprotein structures	1052:1077	3D glycoprotein structures	1052:1077	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	3	19	theme	mass	389:392	arg1	spectrometry					394:405	mass spectrometry	389:405	mass spectrometry	389:405	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	1	20	from	complexity	114:123	arg1	glycoproteins					139:151	glycoproteins	139:151	glycoproteins	139:151	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	2	21	theme	X-ray	307:311	arg1	techniques					295:304	the two most prolific techniques	273:304	the two most prolific techniques: X-ray crystallography and cryo-electron microscopy	273:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	21	theme	X-ray	307:311	arg1	crystallography					313:327	X-ray crystallography	307:327	X-ray crystallography	307:327	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	6	22	theme	3D	1052:1053	arg1	structures					1068:1077	3D glycoprotein structures	1052:1077	3D glycoprotein structures	1052:1077	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	5	23	theme	large	701:705	arg1	initiatives					723:733	several large scale glycomics initiatives	693:733	several large scale glycomics initiatives	693:733	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	1	24	theme	significant	186:196	arg1	challenges					198:207	significant challenges	186:207	significant challenges in structural biology	186:229	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	5	25	theme	API	826:828	arg1	interfaces					830:839	API interfaces	826:839	API interfaces	826:839	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	5	26	theme	scale	707:711	arg1	initiatives					723:733	several large scale glycomics initiatives	693:733	several large scale glycomics initiatives	693:733	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	3	27	theme	full-length	550:560	arg1	composition					569:579	the full-length glycan composition	546:579	precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition	455:579	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	0	28	theme	3D	42:43	arg1	structure					45:53	glycoprotein 3D structure	29:53	glycoprotein 3D structure validation with Privateer	29:79	Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.					
33093930	5	29	theme	glycomics	713:721	arg1	initiatives					723:733	several large scale glycomics initiatives	693:733	several large scale glycomics initiatives	693:733	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	3	30	theme	linkage	592:598	arg1	details					600:606	linkage details	592:606	linkage details for the glycosidic bonds	592:631	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	0	31	theme	glycoprotein	29:40	arg1	structure					45:53	glycoprotein 3D structure	29:53	glycoprotein 3D structure validation with Privateer	29:79	Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.					
33093930	3	32	from	insights	443:450	arg1	information					469:479	precisely the information	455:479	precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition	455:579	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	3	32	from	insights	443:450	arg1	difficult					494:502	difficult	494:502	difficult	494:502	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	1	33	theme	structural	212:221	arg1	biology					223:229	structural biology	212:229	structural biology	212:229	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	6	34	theme	present	849:855	arg1	work					857:860	the present work	845:860	the present work	845:860	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	1	35	gly	glycoproteins	139:151	arg1	glycoproteins					139:151	glycoproteins	139:151	glycoproteins	139:151	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	2	36	dep	techniques	295:304	arg1	microscopy					347:356	cryo-electron microscopy	333:356	cryo-electron microscopy	333:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	36	dep	techniques	295:304	arg1	techniques					295:304	the two most prolific techniques	273:304	the two most prolific techniques: X-ray crystallography and cryo-electron microscopy	273:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	36	dep	techniques	295:304	arg1	crystallography					313:327	X-ray crystallography	307:327	X-ray crystallography	307:327	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	1	37	from	mobility	101:108	arg1	glycoproteins					139:151	glycoproteins	139:151	glycoproteins	139:151	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	3	38	theme	glycosidic	616:625	arg1	bonds					627:631	the glycosidic bonds	612:631	the glycosidic bonds	612:631	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	0	39	gly	glycoprotein	29:40	arg1	glycoprotein					29:40	glycoprotein 3D structure	29:53	glycoprotein 3D structure validation with Privateer	29:79	Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.					
33093930	1	40	from	challenges	198:207	arg1	biology					223:229	structural biology	212:229	structural biology	212:229	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	1	41	theme	glycans	128:134	arg1	complexity					114:123	complexity	114:123	complexity	114:123	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	1	41	theme	glycans	128:134	arg1	mobility					101:108	mobility	101:108	mobility	101:108	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	1	41	theme	glycans	128:134	arg1	heterogeneity					86:98	The heterogeneity	82:98	The heterogeneity	82:98	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	5	42	theme	several	693:699	arg1	initiatives					723:733	several large scale glycomics initiatives	693:733	several large scale glycomics initiatives	693:733	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	3	43	dep	information	469:479	arg1	composition					569:579	the full-length glycan composition	546:579	precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition	455:579	At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds.					
33093930	1	44	from	heterogeneity	86:98	arg1	glycoproteins					139:151	glycoproteins	139:151	glycoproteins	139:151	The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology.					
33093930	2	45	theme	cryo-electron	333:345	arg1	microscopy					347:356	cryo-electron microscopy	333:356	cryo-electron microscopy	333:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	45	theme	cryo-electron	333:345	arg1	techniques					295:304	the two most prolific techniques	273:304	the two most prolific techniques: X-ray crystallography and cryo-electron microscopy	273:356	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	2	46	theme	unique	254:259	arg1	problems					261:268	unique problems	254:268	unique problems	254:268	These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy.					
33093930	0	47	with	structure	45:53	arg1	Privateer					71:79	Privateer	71:79	Privateer	71:79	Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.					
33093930	6	48	theme	structures	1068:1077	arg1	validation					1038:1047	the validation	1034:1047	the validation of 3D glycoprotein structures	1034:1077	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	5	49	theme	available	767:775	arg1	databases					777:785	publicly available databases	758:785	publicly available databases	758:785	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
33093930	6	50	theme	validation	921:930	arg1	software					932:939	the Privateer carbohydrate structure validation software	884:939	the Privateer carbohydrate structure validation software	884:939	In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.					
33093930	5	51	theme	stored	740:745	arg1	results					747:753	stored results	740:753	stored results	740:753	Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces.					
34212492	1	0	theme	metabolite	293:302	arg1	contents					304:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	6	1	theme	Hacıhaliloğlu	1124:1136	arg1	cultivar					1138:1145	the Hacıhaliloğlu cultivar	1120:1145	the Hacıhaliloğlu cultivar	1120:1145	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	6	2	theme	change	998:1003	arg1	patterns					986:993	distinct patterns	977:993	distinct patterns of change	977:1003	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	0	3	theme	Apricot	96:102	arg1	Cultivars					126:134	Two Common Apricot (Prunus armeniaca L.) Cultivars	85:134	Two Common Apricot (Prunus armeniaca L.) Cultivars	85:134	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	0	4	from	Changes	0:6	arg1	Properties					25:34	Compositional Properties	11:34	Compositional Properties	11:34	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	6	5	from	increase	1022:1029	arg1	content					1045:1051	β-carotene content	1034:1051	β-carotene content of Kabaaşı cultivar	1034:1071	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	4	6	theme	phenolic	740:747	arg1	compound					749:756	the only phenolic compound	731:756	the only phenolic compound which increased in concentration during fruit development	731:814	p-Coumaric acid was the only phenolic compound which increased in concentration during fruit development regardless of the cultivar.					
34212492	4	6	theme	phenolic	740:747	arg1	acid					722:725	p-Coumaric acid	711:725	p-Coumaric acid	711:725	p-Coumaric acid was the only phenolic compound which increased in concentration during fruit development regardless of the cultivar.					
34212492	1	7	dep	cultivars	376:384	arg1	Kabaaşı					405:411	Kabaaşı	405:411	Kabaaşı	405:411	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	7	dep	cultivars	376:384	arg1	Hacıhaliloğlu					387:399	Hacıhaliloğlu	387:399	Hacıhaliloğlu	387:399	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	7	dep	cultivars	376:384	arg1	cultivars					376:384	two important apricot cultivars	354:384	two important apricot cultivars (Hacıhaliloğlu and Kabaaşı)	354:412	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	0	8	theme	Common	89:94	arg1	Cultivars					126:134	Two Common Apricot (Prunus armeniaca L.) Cultivars	85:134	Two Common Apricot (Prunus armeniaca L.) Cultivars	85:134	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	1	9	theme	fruit	320:324	arg1	development					326:336	fruit development	320:336	fruit development	320:336	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	10	theme	organic	240:246	arg1	acids					248:252	organic acids	240:252	organic acids	240:252	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	0	11	theme	Prunus	105:110	arg1	Apricot					96:102	Apricot	96:102	Two Common Apricot (Prunus armeniaca L.) Cultivars	85:134	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	0	11	theme	Prunus	105:110	arg1	L.					122:123	Prunus armeniaca L.	105:123	Prunus armeniaca L.	105:123	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	2	12	from	changes	419:425	arg1	properties					448:457	the compositional properties	430:457	the compositional properties of two apricot cultivars	430:482	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	6	13	theme	cultivar	1064:1071	arg1	content					1045:1051	β-carotene content	1034:1051	β-carotene content of Kabaaşı cultivar	1034:1071	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	2	14	theme	cultivars	474:482	arg1	properties					448:457	the compositional properties	430:457	the compositional properties of two apricot cultivars	430:482	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	2	15	theme	compositional	434:446	arg1	properties					448:457	the compositional properties	430:457	the compositional properties of two apricot cultivars	430:482	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	6	16	theme	significant	1164:1174	arg1	change					1176:1181	any significant change	1160:1181	any significant change	1160:1181	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	1	17	theme	present	158:164	arg1	study					166:170	the present study	154:170	the present study	154:170	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	18	theme	study	166:170	arg1	objective					141:149	The objective	137:149	The objective of the present study	137:170	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	19	from	ripening	342:349	arg1	Kabaaşı					405:411	Kabaaşı	405:411	Kabaaşı	405:411	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	19	from	ripening	342:349	arg1	Hacıhaliloğlu					387:399	Hacıhaliloğlu	387:399	Hacıhaliloğlu	387:399	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	19	from	ripening	342:349	arg1	cultivars					376:384	two important apricot cultivars	354:384	two important apricot cultivars (Hacıhaliloğlu and Kabaaşı)	354:412	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	6	20	theme	distinct	977:984	arg1	patterns					986:993	distinct patterns	977:993	distinct patterns of change	977:1003	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	4	21	theme	p-Coumaric	711:720	arg1	compound					749:756	the only phenolic compound	731:756	the only phenolic compound which increased in concentration during fruit development	731:814	p-Coumaric acid was the only phenolic compound which increased in concentration during fruit development regardless of the cultivar.					
34212492	4	21	theme	p-Coumaric	711:720	arg1	acid					722:725	p-Coumaric acid	711:725	p-Coumaric acid	711:725	p-Coumaric acid was the only phenolic compound which increased in concentration during fruit development regardless of the cultivar.					
34212492	3	22	theme	organic	625:631	arg1	acids					633:637	organic acids	625:637	organic acids	625:637	During fruit development, the contents of organic acids and phenolics decreased whereas that of sucrose and sorbitol increased.					
34212492	2	23	from	119 days	559:566	arg1	intervals					538:546	one-week intervals	529:546	one-week intervals from 56 to 119 days	529:566	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	2	24	theme	one-week	529:536	arg1	intervals					538:546	one-week intervals	529:546	one-week intervals from 56 to 119 days	529:566	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	6	25	dep	observed	1077:1084	arg1	such					1005:1008	such	1005:1008	such	1005:1008	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	1	26	from	development	326:336	arg1	Kabaaşı					405:411	Kabaaşı	405:411	Kabaaşı	405:411	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	26	from	development	326:336	arg1	Hacıhaliloğlu					387:399	Hacıhaliloğlu	387:399	Hacıhaliloğlu	387:399	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	26	from	development	326:336	arg1	cultivars					376:384	two important apricot cultivars	354:384	two important apricot cultivars (Hacıhaliloğlu and Kabaaşı)	354:412	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	6	27	theme	cultivars	960:968	arg1	content					945:951	The β-carotene content	930:951	The β-carotene content of the cultivars	930:968	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	0	28	theme	armeniaca	112:120	arg1	Apricot					96:102	Apricot	96:102	Two Common Apricot (Prunus armeniaca L.) Cultivars	85:134	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	0	28	theme	armeniaca	112:120	arg1	L.					122:123	Prunus armeniaca L.	105:123	Prunus armeniaca L.	105:123	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	0	29	theme	Fruit	43:47	arg1	Development					49:59	Fruit Development	43:59	Fruit Development	43:59	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	1	30	theme	secondary	259:267	arg1	contents					304:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	0	31	theme	Cultivars	126:134	arg1	Development					49:59	Fruit Development	43:59	Fruit Development	43:59	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	0	31	theme	Cultivars	126:134	arg1	Ripening					73:80	On-Tree Ripening	65:80	On-Tree Ripening	65:80	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	1	32	dep	primary	220:226	arg1	sugars					229:234	sugars	229:234	sugars	229:234	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	32	dep	primary	220:226	arg1	acids					248:252	organic acids	240:252	organic acids	240:252	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	32	dep	primary	220:226	arg1	β-carotene					281:290	β-carotene	281:290	β-carotene	281:290	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	32	dep	primary	220:226	arg1	phenolics					270:278	phenolics	270:278	phenolics	270:278	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	5	33	theme	other	863:867	arg1	compounds					878:886	the other phenolic compounds	859:886	the other phenolic compounds	859:886	The content of the other phenolic compounds decreased in a cultivar-dependent manner.					
34212492	6	34	theme	β-carotene	1034:1043	arg1	content					1045:1051	β-carotene content	1034:1051	β-carotene content of Kabaaşı cultivar	1034:1071	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	2	35	dep	119 days	559:566	arg1	to					556:557	to	556:557	to	556:557	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	2	36	theme	fruit	506:510	arg1	development					512:522	fruit development	506:522	fruit development	506:522	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	1	37	theme	important	358:366	arg1	Kabaaşı					405:411	Kabaaşı	405:411	Kabaaşı	405:411	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	37	theme	important	358:366	arg1	Hacıhaliloğlu					387:399	Hacıhaliloğlu	387:399	Hacıhaliloğlu	387:399	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	37	theme	important	358:366	arg1	cultivars					376:384	two important apricot cultivars	354:384	two important apricot cultivars (Hacıhaliloğlu and Kabaaşı)	354:412	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	4	38	theme	fruit	798:802	arg1	development					804:814	fruit development	798:814	fruit development	798:814	p-Coumaric acid was the only phenolic compound which increased in concentration during fruit development regardless of the cultivar.					
34212492	1	39	theme	apricot	368:374	arg1	Kabaaşı					405:411	Kabaaşı	405:411	Kabaaşı	405:411	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	39	theme	apricot	368:374	arg1	Hacıhaliloğlu					387:399	Hacıhaliloğlu	387:399	Hacıhaliloğlu	387:399	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	39	theme	apricot	368:374	arg1	cultivars					376:384	two important apricot cultivars	354:384	two important apricot cultivars (Hacıhaliloğlu and Kabaaşı)	354:412	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	1	40	from	variations	195:204	arg1	contents					304:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	6	41	theme	cultivar	1138:1145	arg1	content					1109:1115	the β-carotene content	1094:1115	the β-carotene content of the Hacıhaliloğlu cultivar	1094:1145	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	6	42	theme	β-carotene	934:943	arg1	content					945:951	The β-carotene content	930:951	The β-carotene content of the cultivars	930:968	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	6	43	theme	Kabaaşı	1056:1062	arg1	cultivar					1064:1071	Kabaaşı cultivar	1056:1071	Kabaaşı cultivar	1056:1071	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	3	44	theme	phenolics	643:651	arg1	contents					613:620	the contents	609:620	the contents of organic acids and phenolics	609:651	During fruit development, the contents of organic acids and phenolics decreased whereas that of sucrose and sorbitol increased.					
34212492	5	45	theme	cultivar-dependent	903:920	arg1	manner					922:927	a cultivar-dependent manner	901:927	a cultivar-dependent manner	901:927	The content of the other phenolic compounds decreased in a cultivar-dependent manner.					
34212492	3	46	theme	fruit	590:594	arg1	development					596:606	fruit development	590:606	fruit development	590:606	During fruit development, the contents of organic acids and phenolics decreased whereas that of sucrose and sorbitol increased.					
34212492	3	47	theme	acids	633:637	arg1	contents					613:620	the contents	609:620	the contents of organic acids and phenolics	609:651	During fruit development, the contents of organic acids and phenolics decreased whereas that of sucrose and sorbitol increased.					
34212492	6	48	theme	fruit	1190:1194	arg1	development					1196:1206	fruit development	1190:1206	fruit development	1190:1206	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	6	49	theme	β-carotene	1098:1107	arg1	content					1109:1115	the β-carotene content	1094:1115	the β-carotene content of the Hacıhaliloğlu cultivar	1094:1145	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	6	50	theme	fold	1017:1020	arg1	increase					1022:1029	3 fold increase	1015:1029	3 fold increase in β-carotene content of Kabaaşı cultivar	1015:1071	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	2	51	theme	apricot	466:472	arg1	cultivars					474:482	two apricot cultivars	462:482	two apricot cultivars	462:482	The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one-week intervals from 56 to 119 days after blossom.					
34212492	6	52	dep	showed	970:975	arg1	observed					1077:1084	observed	1077:1084	was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development	1073:1206	The β-carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β-carotene content of Kabaaşı cultivar was observed whereas the β-carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.					
34212492	5	53	theme	phenolic	869:876	arg1	compounds					878:886	the other phenolic compounds	859:886	the other phenolic compounds	859:886	The content of the other phenolic compounds decreased in a cultivar-dependent manner.					
34212492	1	54	theme	major	214:218	arg1	contents					304:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
34212492	0	55	theme	On-Tree	65:71	arg1	Ripening					73:80	On-Tree Ripening	65:80	On-Tree Ripening	65:80	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	0	56	theme	Compositional	11:23	arg1	Properties					25:34	Compositional Properties	11:34	Compositional Properties	11:34	Changes in Compositional Properties during Fruit Development and On-Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars.					
34212492	5	57	theme	compounds	878:886	arg1	content					848:854	The content	844:854	The content of the other phenolic compounds	844:886	The content of the other phenolic compounds decreased in a cultivar-dependent manner.					
34212492	1	58	theme	primary	220:226	arg1	contents					304:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents	209:311	The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β-carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı).					
33233096	2	0	with	Treatment	381:389	arg1	L-theanine					396:405	L-theanine	396:405	L-theanine	396:405	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	8	1	theme	rRNA	1634:1637	arg1	sequencing					1652:1661	16S rRNA phylogenetic sequencing	1630:1661	16S rRNA phylogenetic sequencing	1630:1661	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	9	2	from	disruption	1936:1945	arg1	mice					1950:1953	mice	1950:1953	mice with DSS-induced colitis	1950:1978	Overall, our findings demonstrated that L-theanine inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis.					
33233096	0	3	theme	intestinal	174:183	arg1	disruption					193:202	intestinal barrier disruption	174:202	intestinal barrier disruption	174:202	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	7	4	theme	fatty	1486:1490	arg1	acids					1492:1496	short-chain fatty acids	1474:1496	short-chain fatty acids	1474:1496	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	6	5	theme	cyclooxygenase-2	1212:1227	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	5	theme	cyclooxygenase-2	1212:1227	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	0	6	theme	disruption	193:202	arg1	attenuation					159:169	attenuation	159:169	attenuation of intestinal barrier disruption	159:202	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	0	6	theme	disruption	193:202	arg1	inhibition					118:127	the inhibition	114:127	the inhibition of inflammatory responses	114:153	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	1	7	theme	mucosa	286:291	arg1	injury					293:298	the colonic mucosa injury	274:298	the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	274:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	7	8	from	expression	1530:1539	arg1	tissue					1513:1518	the colonic tissue	1501:1518	the colonic tissue	1501:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	8	from	expression	1530:1539	arg1	colitis					1608:1614	DSS-induced colitis	1596:1614	DSS-induced colitis	1596:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	9	theme	contents	1424:1431	arg1	production					1460:1469	the production	1456:1469	the production of short-chain fatty acids in the colonic tissue	1456:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	9	theme	contents	1424:1431	arg1	expression					1530:1539	gene expression	1525:1539	gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis	1525:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	9	theme	contents	1424:1431	arg1	reduction					1405:1413	the reduction	1401:1413	the reduction of serum contents of diamine oxidase	1401:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	3	10	theme	DSS	783:785	arg1	group					787:791	the DSS group	779:791	the DSS group	779:791	L-Theanine administration also inhibited DSS-induced changes in the colonic tissue that included myeloperoxidase by 4.5-fold and malondialdehyde by 2.3-fold in comparison to the DSS group.					
33233096	6	11	theme	nitric	1189:1194	arg1	synthase					1202:1209	inducible nitric oxide synthase	1179:1209	inducible nitric oxide synthase	1179:1209	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	8	12	theme	significant	1738:1748	arg1	difference					1750:1759	no significant difference	1735:1759	no significant difference	1735:1759	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	6	13	theme	TLR-9	1276:1280	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	13	theme	TLR-9	1276:1280	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	7	14	from	production	1460:1469	arg1	tissue					1513:1518	the colonic tissue	1501:1518	the colonic tissue	1501:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	14	from	production	1460:1469	arg1	colitis					1608:1614	DSS-induced colitis	1596:1614	DSS-induced colitis	1596:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	2	15	theme	colitis	596:602	arg1	features					572:579	the histopathological features	550:579	the histopathological features of DSS-induced colitis	550:602	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	2	15	theme	colitis	596:602	arg1	damage					515:520	the inflammation-associated pathological damage	474:520	the inflammation-associated pathological damage in colon length	474:536	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	3	16	theme	L-Theanine	605:614	arg1	administration					616:629	L-Theanine administration	605:629	L-Theanine administration	605:629	L-Theanine administration also inhibited DSS-induced changes in the colonic tissue that included myeloperoxidase by 4.5-fold and malondialdehyde by 2.3-fold in comparison to the DSS group.					
33233096	10	17	theme	supplementation	2062:2076	arg1	effects					2040:2046	the effects	2036:2046	the effects of L-theanine supplementation on oxidative and inflammatory responses in humans	2036:2126	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	0	18	theme	responses	145:153	arg1	attenuation					159:169	attenuation	159:169	attenuation of intestinal barrier disruption	159:202	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	0	18	theme	responses	145:153	arg1	inhibition					118:127	the inhibition	114:127	the inhibition of inflammatory responses	114:153	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	3	19	theme	DSS-induced	646:656	arg1	changes					658:664	DSS-induced changes	646:664	DSS-induced changes in the colonic tissue that included myeloperoxidase	646:716	L-Theanine administration also inhibited DSS-induced changes in the colonic tissue that included myeloperoxidase by 4.5-fold and malondialdehyde by 2.3-fold in comparison to the DSS group.					
33233096	6	20	theme	receptor	1240:1247	arg1	TLR					1250:1252	receptor (TLR)-2	1240:1255	receptor (TLR)-2	1240:1255	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	10	21	theme	inflammatory	2095:2106	arg1	responses					2108:2116	oxidative and inflammatory responses	2081:2116	oxidative and inflammatory responses in humans	2081:2126	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	7	22	theme	diamine	1436:1442	arg1	oxidase					1444:1450	diamine oxidase	1436:1450	diamine oxidase	1436:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	23	theme	DSS-induced	1596:1606	arg1	colitis					1608:1614	DSS-induced colitis	1596:1614	DSS-induced colitis	1596:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	6	24	theme	toll	1230:1233	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	24	theme	toll	1230:1233	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	10	25	theme	oxidative	2081:2089	arg1	responses					2108:2116	oxidative and inflammatory responses	2081:2116	oxidative and inflammatory responses in humans	2081:2126	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	3	26	theme	colonic	673:679	arg1	tissue					681:686	the colonic tissue	669:686	the colonic tissue	669:686	L-Theanine administration also inhibited DSS-induced changes in the colonic tissue that included myeloperoxidase by 4.5-fold and malondialdehyde by 2.3-fold in comparison to the DSS group.					
33233096	7	27	theme	claudin-1	1583:1591	arg1	production					1460:1469	the production	1456:1469	the production of short-chain fatty acids in the colonic tissue	1456:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	27	theme	claudin-1	1583:1591	arg1	expression					1530:1539	gene expression	1525:1539	gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis	1525:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	27	theme	claudin-1	1583:1591	arg1	reduction					1405:1413	the reduction	1401:1413	the reduction of serum contents of diamine oxidase	1401:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	2	28	theme	histopathological	554:570	arg1	features					572:579	the histopathological features	550:579	the histopathological features of DSS-induced colitis	550:602	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	5	29	theme	tumor	994:998	arg1	factor-α					1009:1016	tumor necrosis factor-α	994:1016	tumor necrosis factor-α	994:1016	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	7	30	theme	barrier	1552:1558	arg1	production					1460:1469	the production	1456:1469	the production of short-chain fatty acids in the colonic tissue	1456:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	30	theme	barrier	1552:1558	arg1	expression					1530:1539	gene expression	1525:1539	gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis	1525:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	30	theme	barrier	1552:1558	arg1	reduction					1405:1413	the reduction	1401:1413	the reduction of serum contents of diamine oxidase	1401:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	10	31	from	effects	2040:2046	arg1	responses					2108:2116	oxidative and inflammatory responses	2081:2116	oxidative and inflammatory responses in humans	2081:2126	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	2	32	from	damage	515:520	arg1	length					531:536	colon length	525:536	colon length	525:536	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	0	33	theme	Ameliorative	0:11	arg1	effects					13:19	Ameliorative effects	0:19	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice	0:92	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	5	34	dep	cytokines	931:939	arg1	expression					941:950	expression	941:950	expression	941:950	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	2	35	theme	activity	443:450	arg1	index					452:456	the disease activity index	431:456	the disease activity index	431:456	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	1	36	theme	C57BL/6J	303:310	arg1	mice					317:320	C57BL/6J male mice	303:320	C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	303:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	6	37	theme	synthase	1202:1209	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	37	theme	synthase	1202:1209	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	2	38	theme	pathological	502:513	arg1	damage					515:520	the inflammation-associated pathological damage	474:520	the inflammation-associated pathological damage in colon length	474:536	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	0	39	theme	sulfate	46:52	arg1	sodium					54:59	dextran sulfate sodium	38:59	dextran sulfate sodium induced colitis in C57BL/6J mice	38:92	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	10	40	from	responses	2108:2116	arg1	humans					2121:2126	humans	2121:2126	humans	2121:2126	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	4	41	theme	lipopolysaccharides	836:854	arg1	level					856:860	lipopolysaccharides level	836:860	lipopolysaccharides level	836:860	In addition, GSH was increased by 85% and lipopolysaccharides level was decreased by 55% in comparison to the DSS group.					
33233096	6	42	theme	TLR-4	1258:1262	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	42	theme	TLR-4	1258:1262	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	9	43	theme	intestinal	1917:1926	arg1	disruption					1936:1945	intestinal barrier disruption	1917:1945	intestinal barrier disruption in mice with DSS-induced colitis	1917:1978	Overall, our findings demonstrated that L-theanine inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis.					
33233096	2	44	from	features	572:579	arg1	length					531:536	colon length	525:536	colon length	525:536	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	6	45	from	increase	1103:1110	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	45	from	increase	1103:1110	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	1	46	theme	L-theanine	244:253	arg1	supplementation					255:269	L-theanine supplementation	244:269	L-theanine supplementation	244:269	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	7	47	theme	acids	1492:1496	arg1	production					1460:1469	the production	1456:1469	the production of short-chain fatty acids in the colonic tissue	1456:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	47	theme	acids	1492:1496	arg1	expression					1530:1539	gene expression	1525:1539	gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis	1525:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	47	theme	acids	1492:1496	arg1	reduction					1405:1413	the reduction	1401:1413	the reduction of serum contents of diamine oxidase	1401:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	6	48	theme	serum	1115:1119	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	0	49	theme	barrier	185:191	arg1	disruption					193:202	intestinal barrier disruption	174:202	intestinal barrier disruption	174:202	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	8	50	theme	microbial	1683:1691	arg1	composition					1703:1713	microbial community composition	1683:1713	microbial community composition induced by DSS	1683:1728	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	7	51	theme	short-chain	1474:1484	arg1	acids					1492:1496	short-chain fatty acids	1474:1496	short-chain fatty acids	1474:1496	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	4	52	theme	DSS	904:906	arg1	group					908:912	the DSS group	900:912	the DSS group	900:912	In addition, GSH was increased by 85% and lipopolysaccharides level was decreased by 55% in comparison to the DSS group.					
33233096	8	53	theme	16S	1630:1632	arg1	sequencing					1652:1661	16S rRNA phylogenetic sequencing	1630:1661	16S rRNA phylogenetic sequencing	1630:1661	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	1	54	theme	colonic	278:284	arg1	mucosa					286:291	the colonic mucosa	274:291	the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	274:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	7	55	theme	oxidase	1444:1450	arg1	contents					1424:1431	serum contents	1418:1431	serum contents of diamine oxidase	1418:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	9	56	theme	DSS-induced	1960:1970	arg1	colitis					1972:1978	DSS-induced colitis	1960:1978	DSS-induced colitis	1960:1978	Overall, our findings demonstrated that L-theanine inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis.					
33233096	8	57	theme	phylogenetic	1639:1650	arg1	sequencing					1652:1661	16S rRNA phylogenetic sequencing	1630:1661	16S rRNA phylogenetic sequencing	1630:1661	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	6	58	theme	TLR-6	1265:1269	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	58	theme	TLR-6	1265:1269	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	59	theme	oxide	1196:1200	arg1	synthase					1202:1209	inducible nitric oxide synthase	1179:1209	inducible nitric oxide synthase	1179:1209	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	10	60	theme	clinical	1989:1996	arg1	trials					1998:2003	Further clinical trials	1981:2003	Further clinical trials	1981:2003	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	1	61	from	injury	293:298	arg1	mice					317:320	C57BL/6J male mice	303:320	C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	303:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	6	62	theme	inducible	1179:1187	arg1	synthase					1202:1209	inducible nitric oxide synthase	1179:1209	inducible nitric oxide synthase	1179:1209	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	63	theme	L-theanine	1334:1343	arg1	administration					1345:1358	L-theanine administration	1334:1358	L-theanine administration	1334:1358	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	5	64	from	levels	1048:1053	arg1	expression					941:950	expression	941:950	expression	941:950	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	7	65	theme	serum	1418:1422	arg1	contents					1424:1431	serum contents	1418:1431	serum contents of diamine oxidase	1418:1450	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	2	66	theme	DSS-induced	584:594	arg1	colitis					596:602	DSS-induced colitis	584:602	DSS-induced colitis	584:602	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	0	67	theme	inflammatory	132:143	arg1	responses					145:153	inflammatory responses	132:153	inflammatory responses	132:153	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	5	68	theme	Proinflammatory	915:929	arg1	cytokines					931:939	Proinflammatory cytokines	915:939	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels	915:1053	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	10	69	theme	L-theanine	2051:2060	arg1	supplementation					2062:2076	L-theanine supplementation	2051:2076	L-theanine supplementation	2051:2076	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	5	70	theme	interleukin	963:973	arg1	IL					976:977	interleukin (IL)-1β	963:981	interleukin (IL)-1β	963:981	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	1	71	from	effects	233:239	arg1	injury					293:298	the colonic mucosa injury	274:298	the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	274:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	7	72	dep	barrier	1552:1558	arg1	occludens-1					1567:1577	zonula occludens-1	1560:1577	zonula occludens-1	1560:1577	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	8	73	theme	L-theanine	1784:1793	arg1	supplementation					1795:1809	L-theanine supplementation	1784:1809	L-theanine supplementation	1784:1809	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	7	74	from	reduction	1405:1413	arg1	tissue					1513:1518	the colonic tissue	1501:1518	the colonic tissue	1501:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	7	74	from	reduction	1405:1413	arg1	colitis					1608:1614	DSS-induced colitis	1596:1614	DSS-induced colitis	1596:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	0	75	theme	C57BL/6J	80:87	arg1	mice					89:92	C57BL/6J mice	80:92	C57BL/6J mice	80:92	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	7	76	theme	zonula	1560:1565	arg1	occludens-1					1567:1577	zonula occludens-1	1560:1577	zonula occludens-1	1560:1577	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	2	77	theme	colon	525:529	arg1	length					531:536	colon length	525:536	colon length	525:536	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	0	78	from	effects	13:19	arg1	sodium					54:59	dextran sulfate sodium	38:59	dextran sulfate sodium induced colitis in C57BL/6J mice	38:92	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	7	79	theme	mucosal	1544:1550	arg1	barrier					1552:1558	mucosal barrier zonula occludens-1 and claudin-1	1544:1591	barrier	1552:1558	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	8	80	from	shift	1674:1678	arg1	composition					1703:1713	microbial community composition	1683:1713	microbial community composition induced by DSS	1683:1728	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	1	81	theme	-induced	363:370	arg1	colitis					372:378	dextran sulfate sodium (DSS)-induced colitis	335:378	dextran sulfate sodium (DSS)-induced colitis	335:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	5	82	theme	necrosis	1000:1007	arg1	factor-α					1009:1016	tumor necrosis factor-α	994:1016	tumor necrosis factor-α	994:1016	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	0	83	theme	L-theanine	24:33	arg1	effects					13:19	Ameliorative effects	0:19	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice	0:92	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	3	84	from	changes	658:664	arg1	tissue					681:686	the colonic tissue	669:686	the colonic tissue	669:686	L-Theanine administration also inhibited DSS-induced changes in the colonic tissue that included myeloperoxidase by 4.5-fold and malondialdehyde by 2.3-fold in comparison to the DSS group.					
33233096	2	85	theme	disease	435:441	arg1	index					452:456	the disease activity index	431:456	the disease activity index	431:456	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	0	86	theme	dextran	38:44	arg1	sodium					54:59	dextran sulfate sodium	38:59	dextran sulfate sodium induced colitis in C57BL/6J mice	38:92	Ameliorative effects of L-theanine on dextran sulfate sodium induced colitis in C57BL/6J mice are associated with the inhibition of inflammatory responses and attenuation of intestinal barrier disruption.					
33233096	1	87	theme	male	312:315	arg1	mice					317:320	C57BL/6J male mice	303:320	C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	303:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
33233096	5	88	theme	protein	1031:1037	arg1	levels					1048:1053	the both protein and mRNA levels	1022:1053	the both protein and mRNA levels	1022:1053	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	9	89	theme	intestinal	1872:1881	arg1	inflammation					1883:1894	intestinal inflammation	1872:1894	intestinal inflammation	1872:1894	Overall, our findings demonstrated that L-theanine inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis.					
33233096	3	90	dep	group	787:791	arg1	comparison					765:774	comparison	765:774	comparison	765:774	L-Theanine administration also inhibited DSS-induced changes in the colonic tissue that included myeloperoxidase by 4.5-fold and malondialdehyde by 2.3-fold in comparison to the DSS group.					
33233096	7	91	theme	gene	1525:1528	arg1	expression					1530:1539	gene expression	1525:1539	gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis	1525:1614	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	5	92	theme	mRNA	1043:1046	arg1	levels					1048:1053	the both protein and mRNA levels	1022:1053	the both protein and mRNA levels	1022:1053	Proinflammatory cytokines expression, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, at the both protein and mRNA levels were also decreased significantly.					
33233096	6	93	theme	colonic	1156:1162	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	2	94	theme	inflammation-associated	478:500	arg1	damage					515:520	the inflammation-associated pathological damage	474:520	the inflammation-associated pathological damage in colon length	474:536	Treatment with L-theanine significantly decreased the disease activity index and ameliorated the inflammation-associated pathological damage in colon length, as well as the histopathological features of DSS-induced colitis.					
33233096	10	95	theme	Further	1981:1987	arg1	trials					1998:2003	Further clinical trials	1981:2003	Further clinical trials	1981:2003	Further clinical trials should be considered to assess the effects of L-theanine supplementation on oxidative and inflammatory responses in humans.					
33233096	6	96	theme	lipopolysaccharides	1132:1150	arg1	expressions					1164:1174	colonic expressions	1156:1174	colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS	1156:1295	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	6	96	theme	lipopolysaccharides	1132:1150	arg1	content					1121:1127	serum content	1115:1127	serum content of lipopolysaccharides	1115:1150	Notably, the increase in serum content of lipopolysaccharides and colonic expressions of inducible nitric oxide synthase, cyclooxygenase-2, toll like receptor (TLR)-2, TLR-4, TLR-6, and TLR-9 induced by DSS were also significantly inhibited by L-theanine administration.					
33233096	7	97	theme	colonic	1505:1511	arg1	tissue					1513:1518	the colonic tissue	1501:1518	the colonic tissue	1501:1518	In addition, L-theanine also attenuated the reduction of serum contents of diamine oxidase and the production of short-chain fatty acids in the colonic tissue, and gene expression of mucosal barrier zonula occludens-1 and claudin-1 in DSS-induced colitis.					
33233096	9	98	theme	barrier	1928:1934	arg1	disruption					1936:1945	intestinal barrier disruption	1917:1945	intestinal barrier disruption in mice with DSS-induced colitis	1917:1978	Overall, our findings demonstrated that L-theanine inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis.					
33233096	9	99	with	mice	1950:1953	arg1	colitis					1972:1978	DSS-induced colitis	1960:1978	DSS-induced colitis	1960:1978	Overall, our findings demonstrated that L-theanine inhibits intestinal inflammation and protects against intestinal barrier disruption in mice with DSS-induced colitis.					
33233096	8	100	theme	community	1693:1701	arg1	composition					1703:1713	microbial community composition	1683:1713	microbial community composition induced by DSS	1683:1728	Furthermore, 16S rRNA phylogenetic sequencing revealed a shift in microbial community composition induced by DSS, but no significant difference was observed following L-theanine supplementation.					
33233096	1	101	theme	supplementation	255:269	arg1	effects					233:239	the effects	229:239	the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis	229:378	This study investigated the effects of L-theanine supplementation on the colonic mucosa injury in C57BL/6J male mice treated with dextran sulfate sodium (DSS)-induced colitis.					
34883361	2	0	theme	cell	515:518	arg1	physiology					520:529	normal microbial cell physiology	498:529	normal microbial cell physiology	498:529	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	1	1	theme	numerous	331:338	arg1	pathogens					361:369	numerous fungal and bacterial pathogens	331:369	numerous fungal and bacterial pathogens	331:369	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	2	2	theme	dispersal	634:642	arg1	agents					644:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	3	3	dep	detection	1077:1085	arg1	the					1073:1075	the	1073:1075	the	1073:1075	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	2	4	theme	microbial	505:513	arg1	physiology					520:529	normal microbial cell physiology	498:529	normal microbial cell physiology	498:529	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	1	5	theme	fungal	340:345	arg1	pathogens					361:369	numerous fungal and bacterial pathogens	331:369	numerous fungal and bacterial pathogens	331:369	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	1	6	from	formation	318:326	arg1	pathogens					361:369	numerous fungal and bacterial pathogens	331:369	numerous fungal and bacterial pathogens	331:369	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	3	7	theme	carbamate	1187:1195	arg1	aglycone					1197:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	8	theme	activity	1133:1140	arg1	detection					1077:1085	detection	1077:1085	detection	1077:1085	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	8	theme	activity	1133:1140	arg1	characterization					1091:1106	characterization	1091:1106	characterization	1091:1106	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	9	theme	glycosyl	715:722	arg1	enzymes					734:740	such glycosyl hydrolase enzymes	710:740	such glycosyl hydrolase enzymes	710:740	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	1	10	theme	critical	247:254	arg1	role					256:259	a critical role	245:259	a critical role	245:259	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	3	11	theme	hydrolase	724:732	arg1	enzymes					734:740	such glycosyl hydrolase enzymes	710:740	such glycosyl hydrolase enzymes	710:740	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	5	12	theme	Escherichia	1594:1604	arg1	lysates					1616:1622	Escherichia coli cell lysates	1594:1622	Escherichia coli cell lysates	1594:1622	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	1	13	theme	bacterial	351:359	arg1	pathogens					361:369	numerous fungal and bacterial pathogens	331:369	numerous fungal and bacterial pathogens	331:369	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	3	14	theme	hexosaminidase	1111:1124	arg1	activity					1133:1140	hexosaminidase enzyme activity	1111:1140	hexosaminidase enzyme activity	1111:1140	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	15	theme	fluorescent	1040:1050	arg1	analog					1062:1067	a new multifunctional fluorescent substrate analog	1018:1067	a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1018:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	4	16	theme	hexosaminidase	1387:1400	arg1	activity					1409:1416	hexosaminidase enzyme activity	1387:1416	hexosaminidase enzyme activity	1387:1416	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	3	17	theme	enzyme	1126:1131	arg1	activity					1133:1140	hexosaminidase enzyme activity	1111:1140	hexosaminidase enzyme activity	1111:1140	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	18	theme	substrate	1052:1060	arg1	analog					1062:1067	a new multifunctional fluorescent substrate analog	1018:1067	a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1018:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	5	19	theme	high-throughput	1654:1668	arg1	assay					1670:1674	a high-throughput assay to screen for DspB inhibitors	1652:1704	a high-throughput assay to screen for DspB inhibitors	1652:1704	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	5	20	theme	DspB	1690:1693	arg1	inhibitors					1695:1704	DspB inhibitors	1690:1704	DspB inhibitors	1690:1704	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	3	21	theme	coumarin	1172:1179	arg1	aglycone					1197:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	1	22	theme	Microbial	107:115	arg1	chitin					183:188	chitin	183:188	chitin	183:188	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	1	22	theme	Microbial	107:115	arg1	peptidoglycan					191:203	peptidoglycan	191:203	peptidoglycan	191:203	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	1	22	theme	Microbial	107:115	arg1	-GlcNAc					223:229	poly-β-(1 → 6)-GlcNAc	209:229	poly-β-(1 → 6)-GlcNAc (dPNAG)	209:237	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	1	22	theme	Microbial	107:115	arg1	polysaccharides					117:131	Microbial polysaccharides	107:131	Microbial polysaccharides	107:131	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	4	23	theme	fluorescent	1447:1457	arg1	measurements					1459:1470	fluorescent measurements	1447:1470	fluorescent measurements	1447:1470	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	0	24	theme	fluorescent	16:26	arg1	probes					28:33	Multifunctional fluorescent probes	0:33	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity	0:104	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity.					
34883361	5	25	theme	assay	1670:1674	arg1	development					1637:1647	the development	1633:1647	the development of a high-throughput assay to screen for DspB inhibitors	1633:1704	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	5	26	theme	probe	1545:1549	arg1	utility					1529:1535	the utility	1525:1535	the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors	1525:1704	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	0	27	theme	Multifunctional	0:14	arg1	probes					28:33	Multifunctional fluorescent probes	0:33	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity	0:104	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity.					
34883361	5	28	theme	recombinant	1565:1575	arg1	activity					1582:1589	recombinant DspB activity	1565:1589	recombinant DspB activity in Escherichia coli cell lysates	1565:1622	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	3	29	theme	7-amino-4-methyl	1155:1170	arg1	aglycone					1197:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	2	30	theme	hydrolase	381:389	arg1	enzymes					391:397	Glycosyl hydrolase enzymes	372:397	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides	372:472	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	2	30	theme	hydrolase	381:389	arg1	biocatalysts					560:571	biocatalysts	560:571	biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents	560:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	2	31	theme	containing	447:456	arg1	polysaccharides					458:472	these β-GlcNAc containing polysaccharides	432:472	these β-GlcNAc containing polysaccharides	432:472	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	1	32	theme	cell	276:279	arg1	integrity					281:289	cell integrity	276:289	cell integrity	276:289	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	4	33	theme	family	1286:1291	arg1	endo-chitinase					1296:1309	family 19 endo-chitinase	1286:1309	family 19 endo-chitinase	1286:1309	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	3	34	theme	analog	1062:1067	arg1	development					1003:1013	the development	999:1013	the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	999:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	2	35	theme	β-GlcNAc	438:445	arg1	polysaccharides					458:472	these β-GlcNAc containing polysaccharides	432:472	these β-GlcNAc containing polysaccharides	432:472	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	4	36	theme	fluorescent	1487:1497	arg1	detection					1499:1507	ratiometric fluorescent detection	1475:1507	ratiometric fluorescent detection	1475:1507	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	2	37	theme	polysaccharides	458:472	arg1	degradation					417:427	the degradation	413:427	the degradation of these β-GlcNAc containing polysaccharides	413:472	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	5	38	from	activity	1582:1589	arg1	lysates					1616:1622	Escherichia coli cell lysates	1594:1622	Escherichia coli cell lysates	1594:1622	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	5	39	theme	DspB	1577:1580	arg1	activity					1582:1589	recombinant DspB activity	1565:1589	recombinant DspB activity in Escherichia coli cell lysates	1565:1622	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	2	40	theme	normal	498:503	arg1	physiology					520:529	normal microbial cell physiology	498:529	normal microbial cell physiology	498:529	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	4	41	theme	ratiometric	1475:1485	arg1	detection					1499:1507	ratiometric fluorescent detection	1475:1507	ratiometric fluorescent detection	1475:1507	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	1	42	dep	play	240:243	arg1	facilitating					297:308	facilitating	297:308	facilitating biofilm formation in numerous fungal and bacterial pathogens	297:369	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	1	42	dep	play	240:243	arg1	maintaining					264:274	maintaining	264:274	maintaining cell integrity	264:289	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	4	43	dep	wavelength	1436:1445	arg1	measurements					1459:1470	fluorescent measurements	1447:1470	fluorescent measurements	1447:1470	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	2	44	theme	biofilm	626:632	arg1	agents					644:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	0	45	theme	high-throughput	39:53	arg1	characterization					55:70	high-throughput characterization	39:70	high-throughput characterization of hexosaminidase enzyme activity	39:104	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity.					
34883361	3	46	dep	pH.	978:980	arg1	present					991:997	present	991:997	present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	991:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	47	theme	AMC	1182:1184	arg1	aglycone					1197:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	4	48	theme	activity	1409:1416	arg1	detection					1374:1382	detection	1374:1382	detection of hexosaminidase enzyme activity	1374:1416	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	48	theme	activity	1409:1416	arg1	substrate					1243:1251	a substrate	1241:1251	a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB)	1241:1360	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	48	theme	activity	1409:1416	arg1	probe					1212:1216	This probe	1207:1216	This probe	1207:1216	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	5	49	dep	Escherichia	1594:1604	arg1	coli					1606:1609	coli	1606:1609	coli	1606:1609	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	0	50	theme	hexosaminidase	75:88	arg1	activity					97:104	hexosaminidase enzyme activity	75:104	hexosaminidase enzyme activity	75:104	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity.					
34883361	4	51	theme	single	1429:1434	arg1	wavelength					1436:1445	either single wavelength fluorescent measurements or ratiometric fluorescent detection	1422:1507	wavelength	1436:1445	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	2	52	theme	anti-bacterial	607:620	arg1	agents					644:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	3	53	dep	Assays	652:657	arg1	characterize					681:692	characterize	681:692	characterize the activity of such glycosyl hydrolase enzymes	681:740	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	53	dep	Assays	652:657	arg1	detect					670:675	detect	670:675	detect	670:675	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	5	54	theme	cell	1611:1614	arg1	lysates					1616:1622	Escherichia coli cell lysates	1594:1622	Escherichia coli cell lysates	1594:1622	We demonstrate the utility of this probe to screen for recombinant DspB activity in Escherichia coli cell lysates, and for the development of a high-throughput assay to screen for DspB inhibitors.					
34883361	2	55	theme	important	479:487	arg1	roles					489:493	important roles	479:493	important roles	479:493	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	1	56	theme	biofilm	310:316	arg1	formation					318:326	biofilm formation	310:326	biofilm formation in numerous fungal and bacterial pathogens	310:369	Microbial polysaccharides composed of N-acetylglucosamine (GlcNAc), such as chitin, peptidoglycan and poly-β-(1 → 6)-GlcNAc (dPNAG), play a critical role in maintaining cell integrity or in facilitating biofilm formation in numerous fungal and bacterial pathogens.					
34883361	4	57	theme	enzyme	1402:1407	arg1	activity					1409:1416	hexosaminidase enzyme activity	1387:1416	hexosaminidase enzyme activity	1387:1416	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	58	theme	hydrolase	1326:1334	arg1	DspB					1356:1359	DspB	1356:1359	DspB	1356:1359	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	58	theme	hydrolase	1326:1334	arg1	B					1353:1353	the dPNAG hydrolase enzyme Dispersin B	1316:1353	the dPNAG hydrolase enzyme Dispersin B (DspB)	1316:1360	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	59	theme	dPNAG	1320:1324	arg1	DspB					1356:1359	DspB	1356:1359	DspB	1356:1359	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	59	theme	dPNAG	1320:1324	arg1	B					1353:1353	the dPNAG hydrolase enzyme Dispersin B	1316:1353	the dPNAG hydrolase enzyme Dispersin B (DspB)	1316:1360	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	60	theme	exo-acting	1257:1266	arg1	β-hexosaminidase					1268:1283	exo-acting β-hexosaminidase	1257:1283	exo-acting β-hexosaminidase	1257:1283	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	2	61	with	biocatalysts	560:571	arg1	applications					578:589	applications	578:589	applications as anti-fungal, anti-bacterial, or biofilm dispersal agents	578:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	3	62	theme	available	810:818	arg1	substrates					910:919	substrates	910:919	substrates	910:919	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	62	theme	available	810:818	arg1	probes					820:825	currently available probes	800:825	currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc)	800:876	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	62	theme	available	810:818	arg1	4-methylumbelliferyl-β-GlcNAc					835:863	4-methylumbelliferyl-β-GlcNAc	835:863	4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc)	835:876	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	2	63	theme	anti-fungal	594:604	arg1	agents					644:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	anti-fungal, anti-bacterial, or biofilm dispersal agents	594:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	3	64	theme	such	710:713	arg1	enzymes					734:740	such glycosyl hydrolase enzymes	710:740	such glycosyl hydrolase enzymes	710:740	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	65	contain	containing	1142:1151	arg1	detection					1077:1085	detection	1077:1085	detection	1077:1085	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	65	contain	containing	1142:1151	arg2	aglycone					1197:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1153:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	4	66	theme	Dispersin	1343:1351	arg1	DspB					1356:1359	DspB	1356:1359	DspB	1356:1359	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	66	theme	Dispersin	1343:1351	arg1	B					1353:1353	the dPNAG hydrolase enzyme Dispersin B	1316:1353	the dPNAG hydrolase enzyme Dispersin B (DspB)	1316:1360	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	3	67	theme	new	1020:1022	arg1	analog					1062:1067	a new multifunctional fluorescent substrate analog	1018:1067	a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1018:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	0	68	theme	activity	97:104	arg1	characterization					55:70	high-throughput characterization	39:70	high-throughput characterization of hexosaminidase enzyme activity	39:104	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity.					
34883361	4	69	theme	enzyme	1336:1341	arg1	DspB					1356:1359	DspB	1356:1359	DspB	1356:1359	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	4	69	theme	enzyme	1336:1341	arg1	B					1353:1353	the dPNAG hydrolase enzyme Dispersin B	1316:1353	the dPNAG hydrolase enzyme Dispersin B (DspB)	1316:1360	This probe is widely tolerated as a substrate for exo-acting β-hexosaminidase, family 19 endo-chitinase, and the dPNAG hydrolase enzyme Dispersin B (DspB) and enables detection of hexosaminidase enzyme activity via either single wavelength fluorescent measurements or ratiometric fluorescent detection.					
34883361	3	70	theme	multifunctional	1024:1038	arg1	analog					1062:1067	a new multifunctional fluorescent substrate analog	1018:1067	a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	1018:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	2	71	theme	Glycosyl	372:379	arg1	enzymes					391:397	Glycosyl hydrolase enzymes	372:397	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides	372:472	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	2	71	theme	Glycosyl	372:379	arg1	biocatalysts					560:571	biocatalysts	560:571	biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents	560:649	Glycosyl hydrolase enzymes that catalyze the degradation of these β-GlcNAc containing polysaccharides play important roles in normal microbial cell physiology and can also be exploited as biocatalysts with applications as anti-fungal, anti-bacterial, or biofilm dispersal agents.					
34883361	0	72	theme	enzyme	90:95	arg1	activity					97:104	hexosaminidase enzyme activity	75:104	hexosaminidase enzyme activity	75:104	Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity.					
34883361	3	73	theme	enzymes	734:740	arg1	activity					698:705	the activity	694:705	the activity of such glycosyl hydrolase enzymes	694:740	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	74	from	changes	967:973	arg1	pH.					978:980	pH.	978:980	pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone	978:1204	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
34883361	3	75	theme	fluorescent	932:942	arg1	signal					944:949	their fluorescent signal	926:949	their fluorescent signal	926:949	Assays to rapidly detect and characterize the activity of such glycosyl hydrolase enzymes can facilitate their development as biocatalyst, however, currently available probes such as 4-methylumbelliferyl-β-GlcNAc (4MU-GlcNAc) are not universally accepted as substrates, and their fluorescent signal is sensitive to changes in pH. Here, we present the development of a new multifunctional fluorescent substrate analog for the detection and characterization of hexosaminidase enzyme activity containing a 7-amino-4-methyl coumarin (AMC) carbamate aglycone.					
32331239	0	0	theme	Fly	100:102	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	3	1	theme	k-carrageenan	352:364	arg1	polymer					366:372	k-carrageenan polymer	352:372	k-carrageenan polymer	352:372	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	5	2	theme	silver	714:719	arg1	nanoparticles					721:733	biologically synthesized silver nanoparticles	689:733	biologically synthesized silver nanoparticles	689:733	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	6	3	dep	fourier	1081:1087	arg1	transform					1089:1097	transform	1089:1097	transform infrared	1089:1106	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	1	4	theme	microbes	197:204	arg1	distribution					151:162	The wide distribution	142:162	The wide distribution of infections-related pathogenic microbes	142:204	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	1	4	theme	microbes	197:204	arg1	related					216:222	related	216:222	related	216:222	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	8	5	from	systems	1453:1459	arg1	homes					1464:1468	homes	1464:1468	homes	1464:1468	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	0	6	theme	Bottle	93:98	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	4	7	theme	bottle	575:580	arg1	fly					582:584	green bottle fly	569:584	green bottle fly	569:584	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	5	8	theme	nanoparticles	721:733	arg1	investigation					672:684	The qualitative investigation	656:684	The qualitative investigation of biologically synthesized silver nanoparticles	656:733	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	6	9	theme	tensile	1160:1166	arg1	strength					1168:1175	tensile strength	1160:1175	tensile strength	1160:1175	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	0	10	theme	Silver	120:125	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	4	11	theme	green	569:573	arg1	fly					582:584	green bottle fly	569:584	green bottle fly	569:584	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	3	12	used	used	378:381	arg2	polymer					366:372	k-carrageenan polymer	352:372	k-carrageenan polymer	352:372	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	8	13	theme	extra	1419:1423	arg1	stage					1425:1429	an extra stage	1416:1429	an extra stage in water purification systems in homes or even at water treatment plants	1416:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	4	14	theme	silver	634:639	arg1	nanoparticles					641:653	silver nanoparticles	634:653	silver nanoparticles	634:653	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	0	15	theme	Bio-Synthesized	104:118	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	7	16	theme	antimicrobial	1182:1194	arg1	activity					1196:1203	The antimicrobial activity	1178:1203	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens	1178:1299	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective.					
32331239	7	16	theme	antimicrobial	1182:1194	arg1	effective					1312:1320	effective	1312:1320	effective	1312:1320	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective.					
32331239	6	17	theme	polymeric	1030:1038	arg1	substrate					1040:1048	the polymeric substrate	1026:1048	the polymeric substrate	1026:1048	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	5	18	theme	nanoparticles	813:825	arg1	size					805:808	the size	801:808	the size of nanoparticles	801:825	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	8	19	theme	probable	1350:1357	arg1	exploitation					1359:1370	the probable exploitation	1346:1370	the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants	1346:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	4	20	used	used	590:593	arg2	agent					607:611	the main agent	598:611	the main agent for the synthesis of silver nanoparticles	598:653	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	4	20	used	used	590:593	arg2	bio-extract					542:552	The bio-extract	538:552	The bio-extract of the pupa of green bottle fly	538:584	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	5	21	theme	qualitative	660:670	arg1	investigation					672:684	The qualitative investigation	656:684	The qualitative investigation of biologically synthesized silver nanoparticles	656:733	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	5	22	theme	transmission	906:917	arg1	TEM					940:942	TEM	940:942	TEM	940:942	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	5	22	theme	transmission	906:917	arg1	microscopy					928:937	transmission electron microscopy	906:937	transmission electron microscopy (TEM)	906:943	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	6	23	dep	transform	1089:1097	arg1	infrared					1099:1106	infrared	1099:1106	transform infrared	1089:1106	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	3	24	theme	strong	465:470	arg1	activity					486:493	strong antimicrobial activity	465:493	strong antimicrobial activity against six pathogenic bacteria and yeast	465:535	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	5	25	theme	scanning	870:877	arg1	SEM					900:902	SEM	900:902	SEM	900:902	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	5	25	theme	scanning	870:877	arg1	microscopy					888:897	scanning electron microscopy	870:897	scanning electron microscopy (SEM)	870:903	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	3	26	theme	pathogenic	507:516	arg1	bacteria					518:525	six pathogenic bacteria	503:525	six pathogenic bacteria	503:525	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	3	27	theme	silver	432:437	arg1	nanoparticles					439:451	the proper/unreleased silver nanoparticles	410:451	the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast	410:535	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	6	28	theme	silver	1000:1005	arg1	nanoparticles					1007:1019	silver nanoparticles	1000:1019	silver nanoparticles	1000:1019	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	8	29	theme	purification	1440:1451	arg1	systems					1453:1459	water purification systems	1434:1459	water purification systems in homes or even at water treatment plants	1434:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	7	30	theme	nanoparticles	1229:1241	arg1	activity					1196:1203	The antimicrobial activity	1178:1203	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens	1178:1299	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective.					
32331239	7	30	theme	nanoparticles	1229:1241	arg1	effective					1312:1320	effective	1312:1320	effective	1312:1320	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective.					
32331239	6	31	theme	thermogravimetric	1117:1133	arg1	TGA					1145:1147	TGA	1145:1147	TGA	1145:1147	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	6	31	theme	thermogravimetric	1117:1133	arg1	analysis					1135:1142	thermogravimetric analysis	1117:1142	thermogravimetric analysis (TGA)	1117:1148	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	0	32	theme	Potential	0:8	arg1	Decontamination					10:24	Potential Decontamination	0:24	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.	0:140	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	4	33	theme	main	602:605	arg1	agent					607:611	the main agent	598:611	the main agent for the synthesis of silver nanoparticles	598:653	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	4	33	theme	main	602:605	arg1	bio-extract					542:552	The bio-extract	538:552	The bio-extract of the pupa of green bottle fly	538:584	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	5	34	theme	synthesized	702:712	arg1	nanoparticles					721:733	biologically synthesized silver nanoparticles	689:733	biologically synthesized silver nanoparticles	689:733	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	8	35	theme	water	1434:1438	arg1	systems					1453:1459	water purification systems	1434:1459	water purification systems in homes or even at water treatment plants	1434:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	0	36	theme	Drinking	29:36	arg1	Pathogens					44:52	Drinking Water Pathogens	29:52	Drinking Water Pathogens	29:52	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	5	37	theme	nm	850:851	arg1	range					834:838	range	834:838	range of 30-100 nm	834:851	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	8	38	from	plants	1497:1502	arg1	stage					1425:1429	an extra stage	1416:1429	an extra stage in water purification systems in homes or even at water treatment plants	1416:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	8	38	from	plants	1497:1502	arg1	systems					1453:1459	water purification systems	1434:1459	water purification systems in homes or even at water treatment plants	1434:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	3	39	theme	proper/unreleased	414:430	arg1	nanoparticles					439:451	the proper/unreleased silver nanoparticles	410:451	the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast	410:535	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	6	40	theme	nanoparticles	1007:1019	arg1	integration					985:995	The proper integration	974:995	The proper integration of silver nanoparticles into the polymeric substrate	974:1048	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	8	41	theme	polymeric/nanoparticles	1379:1401	arg1	exploitation					1359:1370	the probable exploitation	1346:1370	the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants	1346:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	0	42	theme	Pathogens	44:52	arg1	Decontamination					10:24	Potential Decontamination	0:24	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.	0:140	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	5	43	theme	size	959:962	arg1	analyzer					964:971	particle size analyzer	950:971	particle size analyzer	950:971	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	1	44	theme	food	248:251	arg1	contamination					231:243	the contamination	227:243	the contamination of food and/or drinking water	227:273	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	0	45	theme	Water	38:42	arg1	Pathogens					44:52	Drinking Water Pathogens	29:52	Drinking Water Pathogens	29:52	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	3	46	theme	antimicrobial	472:484	arg1	activity					486:493	strong antimicrobial activity	465:493	strong antimicrobial activity against six pathogenic bacteria and yeast	465:535	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	5	47	theme	electron	879:886	arg1	SEM					900:902	SEM	900:902	SEM	900:902	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	5	47	theme	electron	879:886	arg1	microscopy					888:897	scanning electron microscopy	870:897	scanning electron microscopy (SEM)	870:903	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	0	48	theme	k-Carrageenan	62:74	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	4	49	theme	fly	582:584	arg1	pupa					561:564	the pupa	557:564	the pupa of green bottle fly	557:584	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	4	50	theme	pupa	561:564	arg1	agent					607:611	the main agent	598:611	the main agent for the synthesis of silver nanoparticles	598:653	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	4	50	theme	pupa	561:564	arg1	bio-extract					542:552	The bio-extract	538:552	The bio-extract of the pupa of green bottle fly	538:584	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	7	51	theme	yeast	1285:1289	arg1	pathogens					1291:1299	yeast pathogens	1285:1299	yeast pathogens	1285:1299	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective.					
32331239	5	52	theme	particle	950:957	arg1	analyzer					964:971	particle size analyzer	950:971	particle size analyzer	950:971	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	6	53	theme	proper	978:983	arg1	integration					985:995	The proper integration	974:995	The proper integration of silver nanoparticles into the polymeric substrate	974:1048	The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength.					
32331239	5	54	theme	UV-Vis	756:761	arg1	analysis					782:789	UV-Vis spectrophotometric analysis	756:789	UV-Vis spectrophotometric analysis	756:789	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	7	55	theme	k-carrageenan/silver	1208:1227	arg1	nanoparticles					1229:1241	k-carrageenan/silver nanoparticles	1208:1241	k-carrageenan/silver nanoparticles	1208:1241	The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective.					
32331239	1	56	theme	wide	146:149	arg1	distribution					151:162	The wide distribution	142:162	The wide distribution of infections-related pathogenic microbes	142:204	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	1	56	theme	wide	146:149	arg1	related					216:222	related	216:222	related	216:222	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	5	57	theme	spectrophotometric	763:780	arg1	analysis					782:789	UV-Vis spectrophotometric analysis	756:789	UV-Vis spectrophotometric analysis	756:789	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	8	58	theme	treatment	1487:1495	arg1	plants					1497:1502	water treatment plants	1481:1502	water treatment plants	1481:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	1	59	dep	food	248:251	arg1	water					269:273	water	269:273	water	269:273	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	8	60	from	stage	1425:1429	arg1	plants					1497:1502	water treatment plants	1481:1502	water treatment plants	1481:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	8	60	from	stage	1425:1429	arg1	systems					1453:1459	water purification systems	1434:1459	water purification systems in homes or even at water treatment plants	1434:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	1	61	theme	drinking	260:267	arg1	contamination					231:243	the contamination	227:243	the contamination of food and/or drinking water	227:273	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	3	62	theme	current	337:343	arg1	study					345:349	the current study	333:349	the current study	333:349	In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast.					
32331239	8	63	theme	water	1481:1485	arg1	plants					1497:1502	water treatment plants	1481:1502	water treatment plants	1481:1502	These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.					
32331239	4	64	theme	nanoparticles	641:653	arg1	synthesis					621:629	the synthesis	617:629	the synthesis of silver nanoparticles	617:653	The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles.					
32331239	0	65	theme	Green	87:91	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	2	66	theme	applied	288:294	arg1	treatments					296:305	The current applied treatments	276:305	The current applied treatments	276:305	The current applied treatments face some limitations.					
32331239	1	67	theme	infections-related	167:184	arg1	microbes					197:204	infections-related pathogenic microbes	167:204	infections-related pathogenic microbes	167:204	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
32331239	5	68	theme	electron	919:926	arg1	TEM					940:942	TEM	940:942	TEM	940:942	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	5	68	theme	electron	919:926	arg1	microscopy					928:937	transmission electron microscopy	906:937	transmission electron microscopy (TEM)	906:943	The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30-100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer.					
32331239	0	69	theme	Integrated	76:85	arg1	Nanoparticles					127:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles	62:139	Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles.					
32331239	2	70	theme	current	280:286	arg1	treatments					296:305	The current applied treatments	276:305	The current applied treatments	276:305	The current applied treatments face some limitations.					
32331239	1	71	theme	pathogenic	186:195	arg1	microbes					197:204	infections-related pathogenic microbes	167:204	infections-related pathogenic microbes	167:204	The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water.					
33049884	0	0	theme	hydrogen	100:107	arg1	evolution					109:117	enhanced photocatalytic hydrogen evolution	76:117	enhanced photocatalytic hydrogen evolution	76:117	Crystalline CdS/MoS2 shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.					
33049884	8	1	theme	synthesis	1180:1188	arg1	method					1190:1195	the associated synthesis method	1165:1195	the associated synthesis method for PHE	1165:1203	These results show the high potential of b-CdS/MoS2 and the associated synthesis method for PHE.					
33049884	0	2	theme	photocatalytic	85:98	arg1	evolution					109:117	enhanced photocatalytic hydrogen evolution	76:117	enhanced photocatalytic hydrogen evolution	76:117	Crystalline CdS/MoS2 shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.					
33049884	6	3	theme	MoS2	932:935	arg1	evolution					905:913	The photocatalytic H2 evolution	883:913	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2	883:935	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	3	theme	MoS2	932:935	arg1	g-1 h-1					952:958	63.59 mmol g-1 h-1	941:958	63.59 mmol g-1 h-1	941:958	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	3	theme	MoS2	932:935	arg1	PHE					916:918	PHE	916:918	PHE	916:918	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	2	4	with	photocatalysts	318:331	arg1	heterojunctions					338:352	heterojunctions	338:352	heterojunctions preventing fast charge recombination	338:389	For this purpose, higher efficiency is achieved by photocatalysts with heterojunctions preventing fast charge recombination.					
33049884	7	5	theme	higher	1006:1011	arg1	times					973:977	61.1 times	968:977	61.1 times	968:977	It was 61.1 times, 397 times, and 10.2 times higher than PHE with b-CdS, CdS fabricated without BC scaffold, and b-CdS doped with Pt, respectively.					
33049884	3	6	theme	Most	392:395	arg1	processes					397:405	Most processes	392:405	Most processes for the synthesis of high-performance heterojunction photocatalysts	392:473	Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms.					
33049884	4	7	theme	b	541:541	arg1	particles					553:561	berry-shaped (b)-CdS/MoS2 particles	527:561	berry-shaped (b)-CdS/MoS2 particles	527:561	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	6	8	theme	H2	902:903	arg1	evolution					905:913	The photocatalytic H2 evolution	883:913	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2	883:935	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	8	theme	H2	902:903	arg1	g-1 h-1					952:958	63.59 mmol g-1 h-1	941:958	63.59 mmol g-1 h-1	941:958	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	8	theme	H2	902:903	arg1	PHE					916:918	PHE	916:918	PHE	916:918	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	5	9	theme	semiconductor	828:840	arg1	contacts					842:849	intimate semiconductor contacts	819:849	intimate semiconductor contacts beneficial for charge transfer	819:880	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
33049884	6	10	theme	photocatalytic	887:900	arg1	evolution					905:913	The photocatalytic H2 evolution	883:913	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2	883:935	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	10	theme	photocatalytic	887:900	arg1	g-1 h-1					952:958	63.59 mmol g-1 h-1	941:958	63.59 mmol g-1 h-1	941:958	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	10	theme	photocatalytic	887:900	arg1	PHE					916:918	PHE	916:918	PHE	916:918	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	0	11	theme	Crystalline	0:10	arg1	CdS/MoS2					12:19	Crystalline CdS/MoS2	0:19	Crystalline CdS/MoS2	0:19	Crystalline CdS/MoS2 shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.					
33049884	1	12	theme	promising	195:203	arg1	approach					205:212	a promising approach	193:212	a promising approach for the production of easy-to-store chemical energy	193:264	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	1	12	theme	promising	195:203	arg1	conversion					124:133	The conversion	120:133	The conversion of sunlight into H2 by noble-metal-free photocatalysts	120:188	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	3	13	theme	high-performance	428:443	arg1	photocatalysts					460:473	high-performance heterojunction photocatalysts	428:473	high-performance heterojunction photocatalysts	428:473	Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms.					
33049884	4	14	theme	-CdS/MoS2	543:551	arg1	particles					553:561	berry-shaped (b)-CdS/MoS2 particles	527:561	berry-shaped (b)-CdS/MoS2 particles	527:561	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	3	15	theme	heterojunction	445:458	arg1	photocatalysts					460:473	high-performance heterojunction photocatalysts	428:473	high-performance heterojunction photocatalysts	428:473	Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms.					
33049884	4	16	theme	bacterial	629:637	arg1	cellulose					639:647	non-toxic bacterial cellulose	619:647	non-toxic bacterial cellulose	619:647	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	3	17	theme	living	503:508	arg1	organisms					510:518	living organisms	503:518	living organisms	503:518	Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms.					
33049884	4	18	theme	non-toxic	619:627	arg1	cellulose					639:647	non-toxic bacterial cellulose	619:647	non-toxic bacterial cellulose	619:647	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	4	19	theme	spatial	706:712	arg1	arrangement					714:724	enhanced spatial arrangement	697:724	enhanced spatial arrangement	697:724	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	0	20	theme	bacterial	43:51	arg1	scaffold					63:70	a bacterial cellulose scaffold	41:70	a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution	41:117	Crystalline CdS/MoS2 shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.					
33049884	6	21	theme	63.59 mmol	941:950	arg1	evolution					905:913	The photocatalytic H2 evolution	883:913	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2	883:935	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	6	21	theme	63.59 mmol	941:950	arg1	g-1 h-1					952:958	63.59 mmol g-1 h-1	941:958	63.59 mmol g-1 h-1	941:958	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	5	22	theme	suitable	800:807	arg1	shape					809:813	suitable shape	800:813	suitable shape	800:813	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
33049884	3	23	theme	harmful	492:498	arg1	solvents					483:490	solvents	483:490	solvents harmful to living organisms	483:518	Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms.					
33049884	4	24	theme	hydrothermal	592:603	arg1	process					605:611	a hydrothermal process	590:611	a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement	590:724	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	5	25	theme	charge	866:871	arg1	transfer					873:880	charge transfer	866:880	charge transfer	866:880	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
33049884	8	26	theme	associated	1169:1178	arg1	method					1190:1195	the associated synthesis method	1165:1195	the associated synthesis method for PHE	1165:1203	These results show the high potential of b-CdS/MoS2 and the associated synthesis method for PHE.					
33049884	1	27	theme	easy-to-store	236:248	arg1	energy					259:264	easy-to-store chemical energy	236:264	easy-to-store chemical energy	236:264	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	3	28	theme	photocatalysts	460:473	arg1	synthesis					415:423	the synthesis	411:423	the synthesis of high-performance heterojunction photocatalysts	411:473	Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms.					
33049884	4	29	theme	berry-shaped	527:538	arg1	particles					553:561	berry-shaped (b)-CdS/MoS2 particles	527:561	berry-shaped (b)-CdS/MoS2 particles	527:561	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	4	30	with	nanostructures	677:690	arg1	arrangement					714:724	enhanced spatial arrangement	697:724	enhanced spatial arrangement	697:724	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	8	31	theme	b-CdS/MoS2	1150:1159	arg1	potential					1137:1145	the high potential	1128:1145	the high potential of b-CdS/MoS2	1128:1159	These results show the high potential of b-CdS/MoS2 and the associated synthesis method for PHE.					
33049884	8	31	theme	b-CdS/MoS2	1150:1159	arg1	method					1190:1195	the associated synthesis method	1165:1195	the associated synthesis method for PHE	1165:1203	These results show the high potential of b-CdS/MoS2 and the associated synthesis method for PHE.					
33049884	0	32	theme	cellulose	53:61	arg1	scaffold					63:70	a bacterial cellulose scaffold	41:70	a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution	41:117	Crystalline CdS/MoS2 shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.					
33049884	7	33	with	PHE	1018:1020	arg1	CdS					1034:1036	CdS	1034:1036	CdS fabricated without BC scaffold	1034:1067	It was 61.1 times, 397 times, and 10.2 times higher than PHE with b-CdS, CdS fabricated without BC scaffold, and b-CdS doped with Pt, respectively.					
33049884	7	33	with	PHE	1018:1020	arg1	b-CdS					1027:1031	b-CdS	1027:1031	b-CdS	1027:1031	It was 61.1 times, 397 times, and 10.2 times higher than PHE with b-CdS, CdS fabricated without BC scaffold, and b-CdS doped with Pt, respectively.					
33049884	7	33	with	PHE	1018:1020	arg1	b-CdS					1074:1078	b-CdS	1074:1078	b-CdS doped with Pt, respectively	1074:1106	It was 61.1 times, 397 times, and 10.2 times higher than PHE with b-CdS, CdS fabricated without BC scaffold, and b-CdS doped with Pt, respectively.					
33049884	8	34	theme	high	1132:1135	arg1	potential					1137:1145	the high potential	1128:1145	the high potential of b-CdS/MoS2	1128:1159	These results show the high potential of b-CdS/MoS2 and the associated synthesis method for PHE.					
33049884	6	35	theme	%	931:931	arg1	MoS2					932:935	b-CdS/1%MoS2	924:935	b-CdS/1%MoS2	924:935	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	1	36	theme	sunlight	138:145	arg1	approach					205:212	a promising approach	193:212	a promising approach for the production of easy-to-store chemical energy	193:264	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	1	36	theme	sunlight	138:145	arg1	conversion					124:133	The conversion	120:133	The conversion of sunlight into H2 by noble-metal-free photocatalysts	120:188	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	4	37	used	used	653:656	arg2	cellulose					639:647	non-toxic bacterial cellulose	619:647	non-toxic bacterial cellulose	619:647	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	6	38	theme	b-CdS/1	924:930	arg1	%					931:931	%	931:931	%	931:931	The photocatalytic H2 evolution (PHE) of b-CdS/1%MoS2 was 63.59 mmol g-1 h-1.					
33049884	2	39	theme	higher	285:290	arg1	efficiency					292:301	higher efficiency	285:301	higher efficiency	285:301	For this purpose, higher efficiency is achieved by photocatalysts with heterojunctions preventing fast charge recombination.					
33049884	7	40	theme	BC	1057:1058	arg1	scaffold					1060:1067	BC scaffold	1057:1067	BC scaffold	1057:1067	It was 61.1 times, 397 times, and 10.2 times higher than PHE with b-CdS, CdS fabricated without BC scaffold, and b-CdS doped with Pt, respectively.					
33049884	2	41	theme	charge	370:375	arg1	recombination					377:389	fast charge recombination	365:389	fast charge recombination	365:389	For this purpose, higher efficiency is achieved by photocatalysts with heterojunctions preventing fast charge recombination.					
33049884	1	42	theme	chemical	250:257	arg1	energy					259:264	easy-to-store chemical energy	236:264	easy-to-store chemical energy	236:264	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	2	43	theme	fast	365:368	arg1	recombination					377:389	fast charge recombination	365:389	fast charge recombination	365:389	For this purpose, higher efficiency is achieved by photocatalysts with heterojunctions preventing fast charge recombination.					
33049884	1	44	theme	energy	259:264	arg1	production					222:231	the production	218:231	the production of easy-to-store chemical energy	218:264	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	0	45	theme	enhanced	76:83	arg1	evolution					109:117	enhanced photocatalytic hydrogen evolution	76:117	enhanced photocatalytic hydrogen evolution	76:117	Crystalline CdS/MoS2 shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.					
33049884	5	46	with	composite	785:793	arg1	shape					809:813	suitable shape	800:813	suitable shape	800:813	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
33049884	5	46	with	composite	785:793	arg1	contacts					842:849	intimate semiconductor contacts	819:849	intimate semiconductor contacts beneficial for charge transfer	819:880	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
33049884	4	47	theme	enhanced	697:704	arg1	arrangement					714:724	enhanced spatial arrangement	697:724	enhanced spatial arrangement	697:724	Here, berry-shaped (b)-CdS/MoS2 particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement.					
33049884	5	48	theme	beneficial	851:860	arg1	contacts					842:849	intimate semiconductor contacts	819:849	intimate semiconductor contacts beneficial for charge transfer	819:880	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
33049884	1	49	theme	noble-metal-free	158:173	arg1	photocatalysts					175:188	noble-metal-free photocatalysts	158:188	noble-metal-free photocatalysts	158:188	The conversion of sunlight into H2 by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy.					
33049884	5	50	theme	intimate	819:826	arg1	contacts					842:849	intimate semiconductor contacts	819:849	intimate semiconductor contacts beneficial for charge transfer	819:880	Subsequently, MoS2 was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer.					
34083602	7	0	theme	GH	1300:1301	arg1	activity					1303:1310	Mt GH activity	1297:1310	Mt GH activity	1297:1310	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	4	1	theme	commercial	752:761	arg1	mixtures					770:777	crude commercial enzyme mixtures	746:777	crude commercial enzyme mixtures	746:777	Here we integrate these two assays into a single multiplexed assay against both classes of enzymes and use it to characterize crude commercial enzyme mixtures.					
34083602	7	2	theme	oxygen	1322:1327	arg1	conditions					1329:1338	low oxygen conditions	1318:1338	low oxygen conditions	1318:1338	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	8	3	theme	biomass	1512:1518	arg1	pretreatments					1520:1532	biomass pretreatments	1512:1532	biomass pretreatments	1512:1532	Ultimately, this assay can help to discover mixtures of enzymes that could be incorporated into biomass pretreatments to deconstruct diverse components of lignocellulosic biomass.					
34083602	8	4	theme	biomass	1587:1593	arg1	components					1557:1566	diverse components	1549:1566	diverse components of lignocellulosic biomass	1549:1593	Ultimately, this assay can help to discover mixtures of enzymes that could be incorporated into biomass pretreatments to deconstruct diverse components of lignocellulosic biomass.					
34083602	5	5	dep	enzymes	924:930	arg1	Ab					962:963	Ab	962:963	Ab	962:963	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	5	dep	enzymes	924:930	arg1	bisporus					952:959	bisporus	952:959	bisporus	952:959	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	5	dep	enzymes	924:930	arg1	Mt					997:998	Mt	997:998	Mt	997:998	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	5	dep	enzymes	924:930	arg1	thermophila					984:994	Myceliopthora thermophila	970:994	Myceliopthora thermophila (Mt)	970:999	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	4	6	theme	enzyme	763:768	arg1	mixtures					770:777	crude commercial enzyme mixtures	746:777	crude commercial enzyme mixtures	746:777	Here we integrate these two assays into a single multiplexed assay against both classes of enzymes and use it to characterize crude commercial enzyme mixtures.					
34083602	5	7	theme	multiplexed	799:809	arg1	platform					811:818	our multiplexed platform	795:818	our multiplexed platform based on nanostructure-initiator mass spectrometry	795:869	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	6	8	dep	determined	1096:1105	arg1	Using					1066:1070	Using	1066:1070	Using time-series analysis	1066:1091	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	9	from	Ab	1131:1132	arg1	laccase					1118:1124	crude laccase	1112:1124	crude laccase from Ab	1112:1132	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	8	10	theme	lignocellulosic	1571:1585	arg1	biomass					1587:1593	lignocellulosic biomass	1571:1593	lignocellulosic biomass	1571:1593	Ultimately, this assay can help to discover mixtures of enzymes that could be incorporated into biomass pretreatments to deconstruct diverse components of lignocellulosic biomass.					
34083602	7	11	theme	common	1394:1399	arg1	inhibitor					1404:1412	common GH inhibitor	1394:1412	common GH inhibitor	1394:1412	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	7	11	theme	common	1394:1399	arg1	vanillin					1384:1391	vanillin	1384:1391	vanillin (common GH inhibitor)	1384:1413	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	5	12	theme	aromatic	1045:1052	arg1	substrates					1054:1063	aromatic substrates	1045:1063	aromatic substrates	1045:1063	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	4	13	theme	single	662:667	arg1	assay					681:685	a single multiplexed assay	660:685	a single multiplexed assay	660:685	Here we integrate these two assays into a single multiplexed assay against both classes of enzymes and use it to characterize crude commercial enzyme mixtures.					
34083602	0	14	theme	glycosyl	98:105	arg1	hydrolase					107:115	glycosyl hydrolase and lignin modifying enzyme activities	98:154	hydrolase	107:115	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	6	15	contain	has	1134:1136	arg1	laccase					1118:1124	crude laccase	1112:1124	crude laccase from Ab	1112:1132	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	15	contain	has	1134:1136	arg2	activity					1152:1159	the higher GH activity	1138:1159	the higher GH activity	1138:1159	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	16	theme	crude	1112:1116	arg1	laccase					1118:1124	crude laccase	1112:1124	crude laccase from Ab	1112:1132	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	0	17	theme	lignin	121:126	arg1	activities					145:154	glycosyl hydrolase and lignin modifying enzyme activities	98:154	activities	145:154	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	6	18	theme	higher	1142:1147	arg1	activity					1152:1159	the higher GH activity	1138:1159	the higher GH activity	1138:1159	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	3	19	theme	modifying	601:609	arg1	enzymes					611:617	lignin modifying enzymes	594:617	lignin modifying enzymes	594:617	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	2	20	theme	cocktails	429:437	arg1	characterization					384:399	characterization	384:399	characterization	384:399	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	2	20	theme	cocktails	429:437	arg1	development					368:378	development	368:378	development	368:378	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	5	21	from	mixtures	904:911	arg1	fungi					937:941	fungi	937:941	fungi	937:941	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	22	from	activity	1011:1018	arg1	carbohydrate					1028:1039	carbohydrate	1028:1039	carbohydrate	1028:1039	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	22	from	activity	1011:1018	arg1	substrates					1054:1063	aromatic substrates	1045:1063	aromatic substrates	1045:1063	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	8	23	theme	diverse	1549:1555	arg1	components					1557:1566	diverse components	1549:1566	diverse components of lignocellulosic biomass	1549:1593	Ultimately, this assay can help to discover mixtures of enzymes that could be incorporated into biomass pretreatments to deconstruct diverse components of lignocellulosic biomass.					
34083602	7	24	theme	significant	1272:1282	arg1	reduction					1284:1292	a significant reduction	1270:1292	a significant reduction in Mt GH activity	1270:1310	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	0	25	theme	multiplexed	2:12	arg1	assay					63:67	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay	0:67	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.	0:155	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	1	26	theme	major	202:206	arg1	cellulose					221:229	cellulose	221:229	cellulose	221:229	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	1	26	theme	major	202:206	arg1	biopolymers					208:218	three major biopolymers	196:218	three major biopolymers: cellulose, hemicellulose and lignin	196:255	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	1	26	theme	major	202:206	arg1	lignin					250:255	lignin	250:255	lignin	250:255	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	1	26	theme	major	202:206	arg1	hemicellulose					232:244	hemicellulose	232:244	hemicellulose	232:244	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	6	27	theme	time-series	1072:1082	arg1	analysis					1084:1091	time-series analysis	1072:1091	time-series analysis	1072:1091	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	2	28	theme	enzymes/enzyme	414:427	arg1	cocktails					429:437	efficient enzymes/enzyme cocktails	404:437	efficient enzymes/enzyme cocktails	404:437	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	0	29	theme	mass	38:41	arg1	NIMS					57:60	NIMS	57:60	NIMS	57:60	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	0	29	theme	mass	38:41	arg1	spectrometry					43:54	nanostructure-initiator mass spectrometry	14:54	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.	0:155	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	0	30	theme	enzyme	138:143	arg1	activities					145:154	glycosyl hydrolase and lignin modifying enzyme activities	98:154	activities	145:154	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	8	31	theme	enzymes	1472:1478	arg1	mixtures					1460:1467	mixtures	1460:1467	mixtures of enzymes that could be incorporated into biomass pretreatments to deconstruct diverse components of lignocellulosic biomass	1460:1593	Ultimately, this assay can help to discover mixtures of enzymes that could be incorporated into biomass pretreatments to deconstruct diverse components of lignocellulosic biomass.					
34083602	2	32	dep	development	368:378	arg1	the					364:366	the	364:366	the	364:366	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	6	33	theme	GH	1149:1150	arg1	activity					1152:1159	the higher GH activity	1138:1159	the higher GH activity	1138:1159	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	0	34	theme	nanostructure-initiator	14:36	arg1	NIMS					57:60	NIMS	57:60	NIMS	57:60	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	0	34	theme	nanostructure-initiator	14:36	arg1	spectrometry					43:54	nanostructure-initiator mass spectrometry	14:54	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.	0:155	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	0	35	theme	modifying	128:136	arg1	activities					145:154	glycosyl hydrolase and lignin modifying enzyme activities	98:154	activities	145:154	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	2	36	theme	lignin	320:325	arg1	deconstruction					327:340	lignin deconstruction	320:340	lignin deconstruction	320:340	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	3	37	theme	glycosyl	544:551	arg1	hydrolase					553:561	glycosyl hydrolase	544:561	glycosyl hydrolase	544:561	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	7	38	from	reduction	1284:1292	arg1	activity					1303:1310	Mt GH activity	1297:1310	Mt GH activity	1297:1310	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	2	39	theme	Analytical	258:267	arg1	tools					269:273	Analytical tools	258:273	Analytical tools capable of quickly detecting both glycan and lignin deconstruction	258:340	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	3	40	theme	spectrometry-based	498:515	arg1	assays					517:522	nanostructure-initiator mass spectrometry-based assays	469:522	nanostructure-initiator mass spectrometry-based assays	469:522	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	0	41	theme	spectrometry	43:54	arg1	assay					63:67	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay	0:67	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.	0:155	A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities.					
34083602	3	42	theme	nanostructure-initiator	469:491	arg1	assays					517:522	nanostructure-initiator mass spectrometry-based assays	469:522	nanostructure-initiator mass spectrometry-based assays	469:522	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	1	43	dep	biopolymers	208:218	arg1	cellulose					221:229	cellulose	221:229	cellulose	221:229	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	1	43	dep	biopolymers	208:218	arg1	biopolymers					208:218	three major biopolymers	196:218	three major biopolymers: cellulose, hemicellulose and lignin	196:255	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	1	43	dep	biopolymers	208:218	arg1	lignin					250:255	lignin	250:255	lignin	250:255	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	1	43	dep	biopolymers	208:218	arg1	hemicellulose					232:244	hemicellulose	232:244	hemicellulose	232:244	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	4	44	theme	enzymes	711:717	arg1	classes					700:706	both classes	695:706	both classes of enzymes	695:717	Here we integrate these two assays into a single multiplexed assay against both classes of enzymes and use it to characterize crude commercial enzyme mixtures.					
34083602	7	45	theme	low	1318:1320	arg1	conditions					1329:1338	low oxygen conditions	1318:1338	low oxygen conditions	1318:1338	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	5	46	theme	crude	898:902	arg1	mixtures					904:911	crude mixtures	898:911	crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt)	898:999	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	47	theme	laccase	916:922	arg1	enzymes					924:930	laccase enzymes	916:930	laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt)	916:999	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	7	48	theme	Mt	1297:1298	arg1	activity					1303:1310	Mt GH activity	1297:1310	Mt GH activity	1297:1310	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	7	49	theme	Inhibitor	1245:1253	arg1	studies					1255:1261	Inhibitor studies	1245:1261	Inhibitor studies	1245:1261	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	1	50	theme	Lignocellulosic	157:171	arg1	biomass					173:179	Lignocellulosic biomass	157:179	Lignocellulosic biomass	157:179	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin.					
34083602	5	51	from	fungi	937:941	arg1	mixtures					904:911	crude mixtures	898:911	crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt)	898:999	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	5	51	from	fungi	937:941	arg1	enzymes					924:930	laccase enzymes	916:930	laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt)	916:999	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	3	52	theme	hydrolase	553:561	arg1	analysis					532:539	the analysis	528:539	the analysis of glycosyl hydrolase	528:561	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	3	52	theme	hydrolase	553:561	arg1	assay					584:588	an assay	581:588	an assay for lignin modifying enzymes	581:617	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	6	53	theme	model	1229:1233	arg1	compound					1235:1242	our lignin model compound	1218:1242	our lignin model compound	1218:1242	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	54	theme	higher	1194:1199	arg1	activity					1201:1208	the higher activity	1190:1208	the higher activity against our lignin model compound	1190:1242	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	55	contain	has	1186:1188	arg2	activity					1201:1208	the higher activity	1190:1208	the higher activity against our lignin model compound	1190:1242	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	55	contain	has	1186:1188	arg1	laccase					1170:1176	laccase	1170:1176	laccase from Mt	1170:1184	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	6	56	from	Mt	1183:1184	arg1	laccase					1170:1176	laccase	1170:1176	laccase from Mt	1170:1184	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	4	57	theme	crude	746:750	arg1	mixtures					770:777	crude commercial enzyme mixtures	746:777	crude commercial enzyme mixtures	746:777	Here we integrate these two assays into a single multiplexed assay against both classes of enzymes and use it to characterize crude commercial enzyme mixtures.					
34083602	7	58	theme	GH	1401:1402	arg1	inhibitor					1404:1412	common GH inhibitor	1394:1412	common GH inhibitor	1394:1412	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	7	58	theme	GH	1401:1402	arg1	vanillin					1384:1391	vanillin	1384:1391	vanillin (common GH inhibitor)	1384:1413	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	4	59	theme	multiplexed	669:679	arg1	assay					681:685	a single multiplexed assay	660:685	a single multiplexed assay	660:685	Here we integrate these two assays into a single multiplexed assay against both classes of enzymes and use it to characterize crude commercial enzyme mixtures.					
34083602	5	60	theme	nanostructure-initiator	829:851	arg1	spectrometry					858:869	nanostructure-initiator mass spectrometry	829:869	nanostructure-initiator mass spectrometry	829:869	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	7	61	theme	vanillin	1384:1391	arg1	presence					1372:1379	the presence	1368:1379	the presence of vanillin (common GH inhibitor)	1368:1413	Inhibitor studies showed a significant reduction in Mt GH activity under low oxygen conditions and increased activities in the presence of vanillin (common GH inhibitor).					
34083602	2	62	theme	capable	275:281	arg1	tools					269:273	Analytical tools	258:273	Analytical tools capable of quickly detecting both glycan and lignin deconstruction	258:340	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	2	63	theme	efficient	404:412	arg1	cocktails					429:437	efficient enzymes/enzyme cocktails	404:437	efficient enzymes/enzyme cocktails	404:437	Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails.					
34083602	5	64	theme	mass	853:856	arg1	spectrometry					858:869	nanostructure-initiator mass spectrometry	829:869	nanostructure-initiator mass spectrometry	829:869	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	6	65	theme	lignin	1222:1227	arg1	compound					1235:1242	our lignin model compound	1218:1242	our lignin model compound	1218:1242	Using time-series analysis we determined that crude laccase from Ab has the higher GH activity and that laccase from Mt has the higher activity against our lignin model compound.					
34083602	5	66	theme	enzymes	924:930	arg1	mixtures					904:911	crude mixtures	898:911	crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt)	898:999	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34083602	3	67	theme	lignin	594:599	arg1	enzymes					611:617	lignin modifying enzymes	594:617	lignin modifying enzymes	594:617	Previously we have described nanostructure-initiator mass spectrometry-based assays for the analysis of glycosyl hydrolase and most recently an assay for lignin modifying enzymes.					
34083602	5	68	theme	platform	811:818	arg1	Application					780:790	Application	780:790	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry	780:869	Application of our multiplexed platform based on nanostructure-initiator mass spectrometry enabled us to characterize crude mixtures of laccase enzymes from fungi Agaricus bisporus (Ab) and Myceliopthora thermophila (Mt) revealing activity on both carbohydrate and aromatic substrates.					
34390404	7	0	theme	bioink	1460:1465	arg1	formulations					1467:1478	novel bioink formulations	1454:1478	novel bioink formulations	1454:1478	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	6	1	theme	β-TCP	1007:1011	arg1	scaffolds					1013:1021	The sintered, macroporous β-TCP scaffolds	981:1021	scaffolds	1013:1021	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	3	2	theme	printed	494:500	arg1	structure					502:510	the printed structure	490:510	the printed structure	490:510	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	0	3	theme	phosphate	69:77	arg1	scaffolds					79:87	calcium phosphate scaffolds	61:87	calcium phosphate scaffolds	61:87	Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds.					
34390404	3	4	dep	Post-sintering	394:407	arg1	β-TCP					422:426	β-TCP	422:426	β-TCP	422:426	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	3	4	dep	Post-sintering	394:407	arg1	inks					432:435	CMC inks	428:435	CMC inks	428:435	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	3	5	theme	structure	502:510	arg1	size					463:466	uniform grain size	449:466	uniform grain size	449:466	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	3	5	theme	structure	502:510	arg1	shrinkage					477:485	uniform shrinkage	469:485	uniform shrinkage of the printed structure	469:510	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	7	6	theme	manufacturing	1511:1523	arg1	process					1525:1531	a versatile manufacturing process	1499:1531	a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications	1499:1617	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	7	theme	%	690:690	arg1	CMC					692:694	3% CMC	689:694	3% CMC	689:694	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	8	theme	696 ± 108 MPa	952:964	arg1	strength					901:908	compressive strength	889:908	compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa	889:964	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	9	theme	13.1 ± 2.51 MPa	913:927	arg1	strength					901:908	compressive strength	889:908	compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa	889:964	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	4	10	theme	inks	593:596	arg1	properties					575:584	The mechanical properties	560:584	The mechanical properties of the inks	560:596	The mechanical properties of the inks improved with increasing β-TCP content.					
34390404	5	11	dep	gelatin	671:677	arg1	β-TCP					679:683	β-TCP	679:683	β-TCP	679:683	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	11	dep	gelatin	671:677	arg1	CMC					692:694	3% CMC	689:694	3% CMC	689:694	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	12	theme	CMC	656:658	arg1	ink					660:662	β-TCP:CMC ink	650:662	β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength	650:729	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	6	13	theme	phosphate	1149:1157	arg1	scaffolds					1167:1175	calcium phosphate ceramic scaffolds	1141:1175	calcium phosphate ceramic scaffolds manufactured using alternative methods	1141:1214	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	6	13	theme	phosphate	1149:1157	arg1	macroporous					1128:1138	macroporous	1128:1138	macroporous	1128:1138	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	7	14	theme	broad	1538:1542	arg1	applicability					1544:1556	broad applicability	1538:1556	broad applicability in producing substrates suitable for biomedical applications	1538:1617	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	15	dep	ink	660:662	arg1	gelatin					671:677	25:75 gelatin	665:677	25:75 gelatin:β-TCP and 3% CMC	665:694	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	6	16	theme	pore	1059:1062	arg1	size					1064:1067	pore size	1059:1067	pore size	1059:1067	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	5	17	theme	elastic	933:939	arg1	modulus					941:947	elastic modulus	933:947	elastic modulus	933:947	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	1	18	theme	printing-based	178:191	arg1	manufacturing					193:205	direct-ink 3D printing-based manufacturing	164:205	direct-ink 3D printing-based manufacturing of macroporous scaffolds	164:230	Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds.					
34390404	1	19	theme	direct-ink	164:173	arg1	manufacturing					193:205	direct-ink 3D printing-based manufacturing	164:205	direct-ink 3D printing-based manufacturing of macroporous scaffolds	164:230	Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds.					
34390404	5	20	theme	print	746:750	arg1	architectures					760:772	3D print several architectures	743:772	3D print several architectures of macroporous scaffolds	743:797	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	6	21	theme	mechanical	1082:1091	arg1	strength					1093:1100	mechanical strength	1082:1100	mechanical strength	1082:1100	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	5	22	theme	modulus	941:947	arg1	strength					901:908	compressive strength	889:908	compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa	889:964	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	1	23	theme	3D	175:176	arg1	manufacturing					193:205	direct-ink 3D printing-based manufacturing	164:205	direct-ink 3D printing-based manufacturing of macroporous scaffolds	164:230	Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds.					
34390404	5	24	theme	several	752:758	arg1	architectures					760:772	3D print several architectures	743:772	3D print several architectures of macroporous scaffolds	743:797	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	3	25	theme	CMC	428:430	arg1	inks					432:435	CMC inks	428:435	CMC inks	428:435	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	7	26	theme	3D	1410:1411	arg1	printing					1413:1420	3D printing	1410:1420	3D printing	1410:1420	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	27	theme	compressive	889:899	arg1	strength					901:908	compressive strength	889:908	compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa	889:964	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	7	28	theme	high	1221:1224	arg1	porosity					1241:1248	The high interconnected porosity	1217:1248	The high interconnected porosity (45-60%)	1217:1257	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	7	28	theme	high	1221:1224	arg1	%					1256:1256	45-60%	1251:1256	45-60%	1251:1256	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	29	theme	β-TCP	650:654	arg1	ink					660:662	β-TCP:CMC ink	650:662	β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength	650:729	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	6	30	theme	high	1041:1044	arg1	porosity					1046:1053	high porosity	1041:1053	high porosity	1041:1053	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	5	31	dep	gelatin	642:648	arg1	used					735:738	used	735:738	was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved)	731:978	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	6	32	theme	macroporous	995:1005	arg1	scaffolds					1013:1021	The sintered, macroporous β-TCP scaffolds	981:1021	scaffolds	1013:1021	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	7	33	theme	suitable	1582:1589	arg1	substrates					1571:1580	substrates	1571:1580	substrates suitable for biomedical applications	1571:1617	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	34	theme	scaffolds	789:797	arg1	architectures					760:772	3D print several architectures	743:772	3D print several architectures of macroporous scaffolds	743:797	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	0	35	theme	bioinks	15:21	arg1	Development					0:10	Development	0:10	Development	0:10	Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds.					
34390404	4	36	theme	mechanical	564:573	arg1	properties					575:584	The mechanical properties	560:584	The mechanical properties of the inks	560:596	The mechanical properties of the inks improved with increasing β-TCP content.					
34390404	6	37	theme	calcium	1141:1147	arg1	scaffolds					1167:1175	calcium phosphate ceramic scaffolds	1141:1175	calcium phosphate ceramic scaffolds manufactured using alternative methods	1141:1214	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	6	37	theme	calcium	1141:1147	arg1	macroporous					1128:1138	macroporous	1128:1138	macroporous	1128:1138	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	6	38	theme	ceramic	1159:1165	arg1	scaffolds					1167:1175	calcium phosphate ceramic scaffolds	1141:1175	calcium phosphate ceramic scaffolds manufactured using alternative methods	1141:1214	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	6	38	theme	ceramic	1159:1165	arg1	macroporous					1128:1138	macroporous	1128:1138	macroporous	1128:1138	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	5	39	theme	3	689:689	arg1	%					690:690	%	690:690	%	690:690	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	1	40	theme	macroporous	210:220	arg1	scaffolds					222:230	macroporous scaffolds	210:230	macroporous scaffolds	210:230	Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds.					
34390404	7	41	theme	fluid	1263:1267	arg1	conductance					1269:1279	fluid conductance	1263:1279	fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg)	1263:1323	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	1	42	theme	scaffolds	222:230	arg1	manufacturing					193:205	direct-ink 3D printing-based manufacturing	164:205	direct-ink 3D printing-based manufacturing of macroporous scaffolds	164:230	Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds.					
34390404	2	43	theme	gelatin	248:254	arg1	compositions					266:277	the gelatin:β-TCP ink compositions	244:277	the gelatin:β-TCP ink compositions	244:277	Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability.					
34390404	7	44	with	process	1525:1531	arg1	applicability					1544:1556	broad applicability	1538:1556	broad applicability in producing substrates suitable for biomedical applications	1538:1617	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	3	45	theme	uniform	449:455	arg1	size					463:466	uniform grain size	449:466	uniform grain size	449:466	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	3	45	theme	uniform	449:455	arg1	shrinkage					477:485	uniform shrinkage	469:485	uniform shrinkage of the printed structure	469:510	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	5	46	theme	print	814:818	arg1	diameter					831:838	the print nozzle tip diameter	810:838	the print nozzle tip diameter	810:838	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	47	used	used	735:738	arg2	ink					660:662	β-TCP:CMC ink	650:662	β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength	650:729	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	3	48	theme	grain	457:461	arg1	size					463:466	uniform grain size	449:466	uniform grain size	449:466	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	3	48	theme	grain	457:461	arg1	shrinkage					477:485	uniform shrinkage	469:485	uniform shrinkage of the printed structure	469:510	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	5	49	theme	3D	743:744	arg1	architectures					760:772	3D print several architectures	743:772	3D print several architectures of macroporous scaffolds	743:797	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	50	theme	tip	827:829	arg1	diameter					831:838	the print nozzle tip diameter	810:838	the print nozzle tip diameter	810:838	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	7	51	theme	versatile	1501:1509	arg1	process					1525:1531	a versatile manufacturing process	1499:1531	a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications	1499:1617	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	2	52	theme	β-TCP	348:352	arg1	content					354:360	the β-TCP content	344:360	the β-TCP content	344:360	Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability.					
34390404	0	53	theme	microporous	39:49	arg1	printing					30:37	3D printing microporous	27:49	3D printing microporous	27:49	Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds.					
34390404	7	54	theme	biomedical	1595:1604	arg1	applications					1606:1617	biomedical applications	1595:1617	biomedical applications	1595:1617	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	7	55	theme	novel	1454:1458	arg1	formulations					1467:1478	novel bioink formulations	1454:1478	novel bioink formulations	1454:1478	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	56	theme	3D	868:869	arg1	process					880:886	the 3D printing process	864:886	the 3D printing process	864:886	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	7	57	theme	β-TCP	1332:1336	arg1	scaffolds					1338:1346	the β-TCP scaffolds	1328:1346	the β-TCP scaffolds	1328:1346	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	7	58	dep	ability	1364:1370	arg1	tune					1382:1385	tune	1382:1385	to finely tune the architecture using 3D printing	1372:1420	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	6	59	theme	alternative	1196:1206	arg1	methods					1208:1214	alternative methods	1196:1214	alternative methods	1196:1214	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	2	60	theme	compositions	266:277	arg1	Binding					233:239	Binding	233:239	Binding of the gelatin:β-TCP ink compositions	233:277	Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability.					
34390404	1	61	theme	-based	123:128	arg1	bioinks					130:136	Beta-tricalcium phosphate (β-TCP)-based bioinks	90:136	Beta-tricalcium phosphate (β-TCP)-based bioinks	90:136	Beta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds.					
34390404	6	62	theme	sintered	985:992	arg1	scaffolds					1013:1021	The sintered, macroporous β-TCP scaffolds	981:1021	scaffolds	1013:1021	The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods.					
34390404	5	63	theme	printing	871:878	arg1	process					880:886	the 3D printing process	864:886	the 3D printing process	864:886	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	7	64	theme	formulations	1467:1478	arg1	development					1439:1449	the development	1435:1449	the development of novel bioink formulations	1435:1478	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	2	65	theme	ink	262:264	arg1	compositions					266:277	the gelatin:β-TCP ink compositions	244:277	the gelatin:β-TCP ink compositions	244:277	Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability.					
34390404	3	66	theme	uniform	469:475	arg1	size					463:466	uniform grain size	449:466	uniform grain size	449:466	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	3	66	theme	uniform	469:475	arg1	shrinkage					477:485	uniform shrinkage	469:485	uniform shrinkage of the printed structure	469:510	Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic.					
34390404	0	67	theme	calcium	61:67	arg1	scaffolds					79:87	calcium phosphate scaffolds	61:87	calcium phosphate scaffolds	61:87	Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds.					
34390404	2	68	theme	β-TCP	256:260	arg1	compositions					266:277	the gelatin:β-TCP ink compositions	244:277	the gelatin:β-TCP ink compositions	244:277	Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability.					
34390404	5	69	theme	nozzle	820:825	arg1	diameter					831:838	the print nozzle tip diameter	810:838	the print nozzle tip diameter	810:838	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	4	70	theme	β-TCP	623:627	arg1	content					629:635	β-TCP content	623:635	β-TCP content	623:635	The mechanical properties of the inks improved with increasing β-TCP content.					
34390404	5	71	theme	mechanical	711:720	arg1	strength					722:729	mechanical strength	711:729	mechanical strength	711:729	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	7	72	theme	scaffolds	1338:1346	arg1	conductance					1269:1279	fluid conductance	1263:1279	fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg)	1263:1323	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	7	72	theme	scaffolds	1338:1346	arg1	porosity					1241:1248	The high interconnected porosity	1217:1248	The high interconnected porosity (45-60%)	1217:1257	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	7	72	theme	scaffolds	1338:1346	arg1	%					1256:1256	45-60%	1251:1256	45-60%	1251:1256	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	5	73	theme	macroporous	777:787	arg1	scaffolds					789:797	macroporous scaffolds	777:797	macroporous scaffolds	777:797	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	5	74	theme	pore	844:847	arg1	spacing					849:855	pore spacing	844:855	pore spacing	844:855	The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved).					
34390404	7	75	theme	interconnected	1226:1239	arg1	porosity					1241:1248	The high interconnected porosity	1217:1248	The high interconnected porosity (45-60%)	1217:1257	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
34390404	7	75	theme	interconnected	1226:1239	arg1	%					1256:1256	45-60%	1251:1256	45-60%	1251:1256	The high interconnected porosity (45-60%) and fluid conductance (between 1.04 ×10-9 and 2.27 × 10-9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.					
33731303	6	0	theme	CuS	980:982	arg1	nanoparticles					984:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	2	1	theme	@	554:554	arg1	CuS					555:557	HMSNs-CS-DOX@CuS	542:557	HMSNs-CS-DOX@CuS	542:557	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	6	2	theme	photothermal	1040:1051	arg1	%					1074:1074	η = 36.4 %	1065:1074	η = 36.4 %	1065:1074	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	2	theme	photothermal	1040:1051	arg1	properties					1053:1062	potent photothermal properties	1033:1062	potent photothermal properties (η = 36.4 %)	1033:1075	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	16	3	dep	in	2373:2374	arg1	vivo					2376:2379	vivo	2376:2379	vivo	2376:2379	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	12	4	theme	Combination	1852:1862	arg1	therapies					1864:1872	Combination therapies	1852:1872	Combination therapies	1852:1872	Combination therapies, which can ameliorate these issues, are attracting significant attention.					
33731303	2	5	theme	hollow	317:322	arg1	surface					458:464	surface	458:464	surface	458:464	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	5	theme	hollow	317:322	arg1	material					392:399	a base carrier material	377:399	a base carrier material	377:399	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	5	theme	hollow	317:322	arg1	HMSNs					357:361	HMSNs	357:361	HMSNs	357:361	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	5	theme	hollow	317:322	arg1	nanoparticles					342:354	hollow mesoporous silica nanoparticles	317:354	hollow mesoporous silica nanoparticles (HMSNs)	317:362	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	6	6	theme	±	1023:1023	arg1	nm					1028:1029	150 ± 13 nm	1019:1029	150 ± 13 nm	1019:1029	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	6	theme	±	1023:1023	arg1	size					1013:1016	a uniform size	1003:1016	a uniform size (150 ± 13 nm)	1003:1030	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	0	7	theme	Breast Cancer	126:138	arg1	Treatment					113:121	the Treatment	109:121	the Treatment of Breast Cancer	109:138	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	16	8	dep	in	2360:2361	arg1	vitro					2363:2367	vitro	2363:2367	vitro	2363:2367	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	16	9	theme	effective	2447:2455	arg1	therapy					2499:2505	highly effective synergistic chemotherapeutic-photothermal therapy	2440:2505	highly effective synergistic chemotherapeutic-photothermal therapy	2440:2505	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	9	10	theme	animals	1565:1571	arg1	lifetimes					1552:1560	the lifetimes	1548:1560	the lifetimes of animals	1548:1571	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	7	11	contain	has	1232:1234	arg2	biocompatibility					1241:1256	good biocompatibility	1236:1256	good biocompatibility	1236:1256	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	7	11	contain	has	1232:1234	arg1	material					1223:1230	the material	1219:1230	the material	1219:1230	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	3	12	theme	DOX	683:685	arg1	release					672:678	a burst release	664:678	a burst release of DOX into the systemic circulation	664:715	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	6	13	theme	DOX	1149:1151	arg1	release					1153:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	11	14	theme	unavoidable	1819:1829	arg1	toxicity					1842:1849	unavoidable off-target toxicity	1819:1849	unavoidable off-target toxicity	1819:1849	STATEMENT OF SIGNIFICANCE: Conventional cancer chemotherapy is accompanied by unavoidable off-target toxicity.					
33731303	16	15	theme	chemotherapeutic-photothermal	2469:2497	arg1	therapy					2499:2505	highly effective synergistic chemotherapeutic-photothermal therapy	2440:2505	highly effective synergistic chemotherapeutic-photothermal therapy	2440:2505	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	7	16	theme	experimental	1183:1194	arg1	results					1196:1202	In vitro and in vivo experimental results	1162:1202	In vitro and in vivo experimental results	1162:1202	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	6	17	theme	=	1067:1067	arg1	%					1074:1074	η = 36.4 %	1065:1074	η = 36.4 %	1065:1074	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	17	theme	=	1067:1067	arg1	properties					1053:1062	potent photothermal properties	1033:1062	potent photothermal properties (η = 36.4 %)	1033:1075	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	18	theme	laser	1121:1125	arg1	release					1153:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	5	19	theme	potent	936:941	arg1	platform					953:960	a potent chemo/PTT platform	934:960	a potent chemo/PTT platform	934:960	This, coupled with the PTT properties of CuS, results in a potent chemo/PTT platform.					
33731303	16	20	theme	in	2373:2374	arg1	evaluation					2381:2390	a systematic in vitro and in vivo evaluation	2347:2390	a systematic in vitro and in vivo evaluation	2347:2390	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	9	21	theme	breast	1585:1590	arg1	model					1599:1603	a murine breast cancer model	1576:1603	a murine breast cancer model	1576:1603	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	6	22	contain	have	998:1001	arg2	release					1153:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	22	contain	have	998:1001	arg2	nm					1028:1029	150 ± 13 nm	1019:1029	150 ± 13 nm	1019:1029	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	22	contain	have	998:1001	arg1	nanoparticles					984:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	22	contain	have	998:1001	arg2	properties					1053:1062	potent photothermal properties	1033:1062	potent photothermal properties (η = 36.4 %)	1033:1075	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	22	contain	have	998:1001	arg2	size					1013:1016	a uniform size	1003:1016	a uniform size (150 ± 13 nm)	1003:1030	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	22	contain	have	998:1001	arg2	%					1074:1074	η = 36.4 %	1065:1074	η = 36.4 %	1065:1074	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	7	23	theme	in	1175:1176	arg1	results					1196:1202	In vitro and in vivo experimental results	1162:1202	In vitro and in vivo experimental results	1162:1202	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	7	24	dep	In	1162:1163	arg1	vitro					1165:1169	vitro	1165:1169	vitro	1165:1169	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	10	25	theme	further	1657:1663	arg1	investigation					1665:1677	further investigation	1657:1677	further investigation	1657:1677	The system developed in this work therefore merits further investigation as a potential nanotheranostic platform for cancer treatment.					
33731303	1	26	theme	chemotherapy	160:171	arg1	combination					145:155	The combination	141:155	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation	141:228	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	8	27	theme	CuS	1318:1320	arg1	nanodots					1322:1329	the CuS nanodots	1314:1329	the CuS nanodots	1314:1329	Moreover, the CuS nanodots permit simultaneous thermal/photoacoustic dual-modality imaging.					
33731303	4	28	theme	reducing	803:810	arg1	microenvironment					812:827	the reducing microenvironment	799:827	the reducing microenvironment of the tumor	799:840	S-S bonds connect the CuS dots to the HMSNs; these are selectively cleaved under the reducing microenvironment of the tumor, permitting targeted drug release.					
33731303	0	29	theme	Theranostic	86:96	arg1	Agents					98:103	Theranostic Agents	86:103	Theranostic Agents for the Treatment of Breast Cancer	86:138	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	13	30	theme	anticancer	1958:1967	arg1	DOX					1987:1989	DOX	1987:1989	DOX	1987:1989	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	13	30	theme	anticancer	1958:1967	arg1	doxorubicin					1974:1984	the anticancer drug doxorubicin	1954:1984	the anticancer drug doxorubicin (DOX)	1954:1990	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	15	31	theme	infrared	2201:2208	arg1	irradiation					2222:2232	near infrared laser (NIR) irradiation	2196:2232	near infrared laser (NIR) irradiation	2196:2232	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	1	32	theme	photothermal	177:188	arg1	PTT					199:201	PTT	199:201	PTT	199:201	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	1	32	theme	photothermal	177:188	arg1	therapy					190:196	photothermal therapy	177:196	photothermal therapy (PTT)	177:202	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	4	33	theme	targeted	854:861	arg1	release					868:874	targeted drug release	854:874	targeted drug release	854:874	S-S bonds connect the CuS dots to the HMSNs; these are selectively cleaved under the reducing microenvironment of the tumor, permitting targeted drug release.					
33731303	16	34	theme	@	2418:2418	arg1	platform					2423:2430	the HMSNs-CS-DOX@CuS platform	2402:2430	the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties	2402:2570	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	8	35	theme	thermal/photoacoustic	1351:1371	arg1	imaging					1387:1393	simultaneous thermal/photoacoustic dual-modality imaging	1338:1393	simultaneous thermal/photoacoustic dual-modality imaging	1338:1393	Moreover, the CuS nanodots permit simultaneous thermal/photoacoustic dual-modality imaging.					
33731303	17	36	theme	simultaneous	2650:2661	arg1	imaging					2690:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	9	37	theme	@	1423:1423	arg1	CuS					1424:1426	HMSNs-CS-DOX@CuS	1411:1426	HMSNs-CS-DOX@CuS	1411:1426	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	17	38	theme	NIR-enhanced	2612:2623	arg1	therapy					2637:2643	NIR-enhanced synergistic therapy	2612:2643	NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging	2612:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	2	39	theme	base	379:382	arg1	material					392:399	a base carrier material	377:399	a base carrier material	377:399	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	39	theme	base	379:382	arg1	nanoparticles					342:354	hollow mesoporous silica nanoparticles	317:354	hollow mesoporous silica nanoparticles (HMSNs)	317:362	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	0	40	theme	Hollow	0:5	arg1	Silica					18:23	Hollow Mesoporous Silica	0:23	Hollow Mesoporous Silica	0:23	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	15	41	theme	laser	2210:2214	arg1	irradiation					2222:2232	near infrared laser (NIR) irradiation	2196:2232	near infrared laser (NIR) irradiation	2196:2232	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	9	42	from	apoptosis	1465:1473	arg1	cells					1485:1489	cancer cells	1478:1489	cancer cells	1478:1489	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	7	43	theme	In	1162:1163	arg1	results					1196:1202	In vitro and in vivo experimental results	1162:1202	In vitro and in vivo experimental results	1162:1202	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	2	44	theme	drug	430:433	arg1	doxorubicin					435:445	the anti-cancer drug doxorubicin	414:445	the anti-cancer drug doxorubicin (DOX)	414:451	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	44	theme	drug	430:433	arg1	DOX					448:450	DOX	448:450	DOX	448:450	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	15	45	from	surface	2263:2269	arg1	located					2248:2254	located	2248:2254	located	2248:2254	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	11	46	theme	cancer	1781:1786	arg1	chemotherapy					1788:1799	Conventional cancer chemotherapy	1768:1799	Conventional cancer chemotherapy	1768:1799	STATEMENT OF SIGNIFICANCE: Conventional cancer chemotherapy is accompanied by unavoidable off-target toxicity.					
33731303	13	47	theme	hollow	2057:2062	arg1	HMSNs					2097:2101	HMSNs	2097:2101	HMSNs	2097:2101	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	13	47	theme	hollow	2057:2062	arg1	nanoparticles					2082:2094	hollow mesoporous silica nanoparticles	2057:2094	chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs)	2034:2102	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	9	48	theme	extensive	1455:1463	arg1	apoptosis					1465:1473	extensive apoptosis	1455:1473	extensive apoptosis in cancer cells	1455:1489	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	17	49	theme	dual	2685:2688	arg1	imaging					2690:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	10	50	theme	potential	1684:1692	arg1	platform					1710:1717	a potential nanotheranostic platform	1682:1717	a potential nanotheranostic platform for cancer treatment	1682:1738	The system developed in this work therefore merits further investigation as a potential nanotheranostic platform for cancer treatment.					
33731303	0	51	theme	Chitosan-Copper	48:62	arg1	Composites					72:81	Chitosan-Copper Sulfide Composites	48:81	Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer	48:138	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	13	52	theme	silica	2075:2080	arg1	HMSNs					2097:2101	HMSNs	2097:2101	HMSNs	2097:2101	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	13	52	theme	silica	2075:2080	arg1	nanoparticles					2082:2094	hollow mesoporous silica nanoparticles	2057:2094	chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs)	2034:2102	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	16	53	theme	in	2360:2361	arg1	evaluation					2381:2390	a systematic in vitro and in vivo evaluation	2347:2390	a systematic in vitro and in vivo evaluation	2347:2390	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	5	54	theme	CuS	918:920	arg1	properties					904:913	the PTT properties	896:913	the PTT properties of CuS	896:920	This, coupled with the PTT properties of CuS, results in a potent chemo/PTT platform.					
33731303	16	55	theme	effective	2516:2524	arg1	properties					2561:2570	effective thermal/photoacoustic dual-imaging properties	2516:2570	effective thermal/photoacoustic dual-imaging properties	2516:2570	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	10	56	theme	cancer	1723:1728	arg1	treatment					1730:1738	cancer treatment	1723:1738	cancer treatment	1723:1738	The system developed in this work therefore merits further investigation as a potential nanotheranostic platform for cancer treatment.					
33731303	1	57	theme	increasing	244:253	arg1	attention					255:263	increasing attention	244:263	increasing attention	244:263	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	1	57	theme	increasing	244:253	arg1	strategy					270:277	a strategy	268:277	a strategy for enhancing cancer treatment	268:308	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	16	58	theme	dual-imaging	2548:2559	arg1	properties					2561:2570	effective thermal/photoacoustic dual-imaging properties	2516:2570	effective thermal/photoacoustic dual-imaging properties	2516:2570	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	15	59	theme	NIR	2217:2219	arg1	irradiation					2222:2232	near infrared laser (NIR) irradiation	2196:2232	near infrared laser (NIR) irradiation	2196:2232	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	17	60	with	therapy	2637:2643	arg1	imaging					2690:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	13	61	theme	central	2016:2022	arg1	cavity					2024:2029	the central cavity	2012:2029	the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs)	2012:2102	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	6	62	theme	@	979:979	arg1	nanoparticles					984:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	3	63	theme	surface	625:631	arg1	pores					633:637	the surface pores	621:637	the surface pores of the HMSNs	621:650	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	6	64	dep	infrared	1106:1113	arg1	NIR					1116:1118	NIR	1116:1118	NIR	1116:1118	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	7	65	theme	cancer	1290:1295	arg1	cells					1297:1301	cancer cells	1290:1301	cancer cells	1290:1301	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	2	66	theme	HMSNs-CS-DOX	542:553	arg1	CuS					555:557	HMSNs-CS-DOX@CuS	542:557	HMSNs-CS-DOX@CuS	542:557	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	6	67	theme	potent	1033:1038	arg1	%					1074:1074	η = 36.4 %	1065:1074	η = 36.4 %	1065:1074	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	67	theme	potent	1033:1038	arg1	properties					1053:1062	potent photothermal properties	1033:1062	potent photothermal properties (η = 36.4 %)	1033:1075	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	68	theme	near	1101:1104	arg1	release					1153:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	1	69	theme	therapy	190:196	arg1	combination					145:155	The combination	141:155	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation	141:228	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	17	70	theme	new	2597:2599	arg1	avenue					2601:2606	a new avenue	2595:2606	a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging	2595:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	3	71	theme	HMSNs	646:650	arg1	pores					633:637	the surface pores	621:637	the surface pores of the HMSNs	621:650	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	6	72	theme	tumor-targeted	1082:1095	arg1	release					1153:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	2	73	theme	mesoporous	324:333	arg1	surface					458:464	surface	458:464	surface	458:464	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	73	theme	mesoporous	324:333	arg1	material					392:399	a base carrier material	377:399	a base carrier material	377:399	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	73	theme	mesoporous	324:333	arg1	HMSNs					357:361	HMSNs	357:361	HMSNs	357:361	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	73	theme	mesoporous	324:333	arg1	nanoparticles					342:354	hollow mesoporous silica nanoparticles	317:354	hollow mesoporous silica nanoparticles (HMSNs)	317:362	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	7	74	dep	in	1175:1176	arg1	vivo					1178:1181	vivo	1178:1181	vivo	1178:1181	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	7	75	theme	good	1236:1239	arg1	biocompatibility					1241:1256	good biocompatibility	1236:1256	good biocompatibility	1236:1256	In vitro and in vivo experimental results confirmed that the material has good biocompatibility, but is effectively taken up by cancer cells.					
33731303	3	76	theme	burst	666:670	arg1	release					672:678	a burst release	664:678	a burst release of DOX into the systemic circulation	664:715	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	11	77	theme	off-target	1831:1840	arg1	toxicity					1842:1849	unavoidable off-target toxicity	1819:1849	unavoidable off-target toxicity	1819:1849	STATEMENT OF SIGNIFICANCE: Conventional cancer chemotherapy is accompanied by unavoidable off-target toxicity.					
33731303	1	78	theme	single	211:216	arg1	formulation					218:228	a single formulation	209:228	a single formulation	209:228	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	16	79	theme	synergistic	2457:2467	arg1	therapy					2499:2505	highly effective synergistic chemotherapeutic-photothermal therapy	2440:2505	highly effective synergistic chemotherapeutic-photothermal therapy	2440:2505	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	4	80	theme	tumor	836:840	arg1	microenvironment					812:827	the reducing microenvironment	799:827	the reducing microenvironment of the tumor	799:840	S-S bonds connect the CuS dots to the HMSNs; these are selectively cleaved under the reducing microenvironment of the tumor, permitting targeted drug release.					
33731303	9	81	with	Treatment	1396:1404	arg1	CuS					1424:1426	HMSNs-CS-DOX@CuS	1411:1426	HMSNs-CS-DOX@CuS	1411:1426	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	9	81	with	Treatment	1396:1404	arg1	irradiation					1436:1446	NIR irradiation	1432:1446	NIR irradiation	1432:1446	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	3	82	theme	systemic	696:703	arg1	circulation					705:715	the systemic circulation	692:715	the systemic circulation	692:715	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	3	83	theme	CuS	585:587	arg1	dots					589:592	the CuS dots	581:592	the CuS dots	581:592	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	3	83	theme	CuS	585:587	arg1	gatekeepers					601:611	gatekeepers	601:611	gatekeepers	601:611	In this formulation, the CuS dots act as gatekeepers to seal the surface pores of the HMSNs, preventing a burst release of DOX into the systemic circulation.					
33731303	6	84	theme	irradiation-triggered	1127:1147	arg1	release					1153:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release	1082:1159	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	9	85	theme	cancer	1478:1483	arg1	cells					1485:1489	cancer cells	1478:1489	cancer cells	1478:1489	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	15	86	theme	drug	2320:2323	arg1	release					2325:2331	drug release	2320:2331	drug release	2320:2331	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	9	87	theme	murine	1578:1583	arg1	model					1599:1603	a murine breast cancer model	1576:1603	a murine breast cancer model	1576:1603	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	6	88	theme	η	1065:1065	arg1	%					1074:1074	η = 36.4 %	1065:1074	η = 36.4 %	1065:1074	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	88	theme	η	1065:1065	arg1	properties					1053:1062	potent photothermal properties	1033:1062	potent photothermal properties (η = 36.4 %)	1033:1075	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	16	89	theme	thermal/photoacoustic	2526:2546	arg1	properties					2561:2570	effective thermal/photoacoustic dual-imaging properties	2516:2570	effective thermal/photoacoustic dual-imaging properties	2516:2570	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	5	90	theme	chemo/PTT	943:951	arg1	platform					953:960	a potent chemo/PTT platform	934:960	a potent chemo/PTT platform	934:960	This, coupled with the PTT properties of CuS, results in a potent chemo/PTT platform.					
33731303	9	91	theme	cancer	1592:1597	arg1	model					1599:1603	a murine breast cancer model	1576:1603	a murine breast cancer model	1576:1603	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	12	92	theme	significant	1925:1935	arg1	attention					1937:1945	significant attention	1925:1945	significant attention	1925:1945	Combination therapies, which can ameliorate these issues, are attracting significant attention.					
33731303	16	93	theme	HMSNs-CS-DOX	2406:2417	arg1	platform					2423:2430	the HMSNs-CS-DOX@CuS platform	2402:2430	the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties	2402:2570	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	8	94	theme	simultaneous	1338:1349	arg1	imaging					1387:1393	simultaneous thermal/photoacoustic dual-modality imaging	1338:1393	simultaneous thermal/photoacoustic dual-modality imaging	1338:1393	Moreover, the CuS nanodots permit simultaneous thermal/photoacoustic dual-modality imaging.					
33731303	14	95	theme	tumor	2156:2160	arg1	microenvironment					2162:2177	the tumor microenvironment	2152:2177	the tumor microenvironment	2152:2177	The prepared system can target drug release to the tumor microenvironment.					
33731303	13	96	theme	drug	1969:1972	arg1	DOX					1987:1989	DOX	1987:1989	DOX	1987:1989	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	13	96	theme	drug	1969:1972	arg1	doxorubicin					1974:1984	the anticancer drug doxorubicin	1954:1984	the anticancer drug doxorubicin (DOX)	1954:1990	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	16	97	theme	CuS	2419:2421	arg1	platform					2423:2430	the HMSNs-CS-DOX@CuS platform	2402:2430	the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties	2402:2570	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	8	98	theme	dual-modality	1373:1385	arg1	imaging					1387:1393	simultaneous thermal/photoacoustic dual-modality imaging	1338:1393	simultaneous thermal/photoacoustic dual-modality imaging	1338:1393	Moreover, the CuS nanodots permit simultaneous thermal/photoacoustic dual-modality imaging.					
33731303	2	99	used	used	369:372	arg2	HMSNs					357:361	HMSNs	357:361	HMSNs	357:361	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	99	used	used	369:372	arg2	material					392:399	a base carrier material	377:399	a base carrier material	377:399	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	99	used	used	369:372	arg2	nanoparticles					342:354	hollow mesoporous silica nanoparticles	317:354	hollow mesoporous silica nanoparticles (HMSNs)	317:362	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	99	used	used	369:372	arg2	surface					458:464	surface	458:464	surface	458:464	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	9	100	theme	HMSNs-CS-DOX	1411:1422	arg1	CuS					1424:1426	HMSNs-CS-DOX@CuS	1411:1426	HMSNs-CS-DOX@CuS	1411:1426	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	6	101	theme	HMSNs-CS-DOX	967:978	arg1	nanoparticles					984:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles	963:996	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	4	102	theme	drug	863:866	arg1	release					868:874	targeted drug release	854:874	targeted drug release	854:874	S-S bonds connect the CuS dots to the HMSNs; these are selectively cleaved under the reducing microenvironment of the tumor, permitting targeted drug release.					
33731303	14	103	theme	drug	2136:2139	arg1	release					2141:2147	drug release	2136:2147	drug release	2136:2147	The prepared system can target drug release to the tumor microenvironment.					
33731303	2	104	theme	carrier	384:390	arg1	material					392:399	a base carrier material	377:399	a base carrier material	377:399	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	104	theme	carrier	384:390	arg1	nanoparticles					342:354	hollow mesoporous silica nanoparticles	317:354	hollow mesoporous silica nanoparticles (HMSNs)	317:362	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	0	105	theme	Mesoporous	7:16	arg1	Silica					18:23	Hollow Mesoporous Silica	0:23	Hollow Mesoporous Silica	0:23	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	15	106	theme	located	2248:2254	arg1	pores					2271:2275	located at the surface pores of the HMSNs generate energy	2248:2304	located at the surface pores of the HMSNs generate energy	2248:2304	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	17	107	theme	synergistic	2625:2635	arg1	therapy					2637:2643	NIR-enhanced synergistic therapy	2612:2643	NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging	2612:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	11	108	theme	SIGNIFICANCE	1754:1765	arg1	STATEMENT					1741:1749	STATEMENT	1741:1749	STATEMENT OF SIGNIFICANCE	1741:1765	STATEMENT OF SIGNIFICANCE: Conventional cancer chemotherapy is accompanied by unavoidable off-target toxicity.					
33731303	1	109	theme	cancer	293:298	arg1	treatment					300:308	cancer treatment	293:308	cancer treatment	293:308	The combination of chemotherapy and photothermal therapy (PTT) into a single formulation has attracted increasing attention as a strategy for enhancing cancer treatment.					
33731303	9	110	theme	NIR	1432:1434	arg1	irradiation					1436:1446	NIR irradiation	1432:1446	NIR irradiation	1432:1446	Treatment with HMSNs-CS-DOX@CuS and NIR irradiation caused extensive apoptosis in cancer cells both in vitro and in vivo, and could dramatically extend the lifetimes of animals in a murine breast cancer model.					
33731303	2	111	theme	anti-cancer	418:428	arg1	doxorubicin					435:445	the anti-cancer drug doxorubicin	414:445	the anti-cancer drug doxorubicin (DOX)	414:451	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	111	theme	anti-cancer	418:428	arg1	DOX					448:450	DOX	448:450	DOX	448:450	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	14	112	theme	prepared	2109:2116	arg1	system					2118:2123	The prepared system	2105:2123	The prepared system	2105:2123	The prepared system can target drug release to the tumor microenvironment.					
33731303	17	113	theme	thermal/photoacoustic	2663:2683	arg1	imaging					2690:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	simultaneous thermal/photoacoustic dual imaging	2650:2696	This work may open up a new avenue for NIR-enhanced synergistic therapy with simultaneous thermal/photoacoustic dual imaging.					
33731303	10	114	theme	nanotheranostic	1694:1708	arg1	platform					1710:1717	a potential nanotheranostic platform	1682:1717	a potential nanotheranostic platform for cancer treatment	1682:1738	The system developed in this work therefore merits further investigation as a potential nanotheranostic platform for cancer treatment.					
33731303	0	115	theme	Sulfide	64:70	arg1	Composites					72:81	Chitosan-Copper Sulfide Composites	48:81	Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer	48:138	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	13	116	theme	mesoporous	2064:2073	arg1	HMSNs					2097:2101	HMSNs	2097:2101	HMSNs	2097:2101	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	13	116	theme	mesoporous	2064:2073	arg1	nanoparticles					2082:2094	hollow mesoporous silica nanoparticles	2057:2094	chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs)	2034:2102	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	2	117	theme	copper	504:509	arg1	CuS					520:522	CuS	520:522	CuS	520:522	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	117	theme	copper	504:509	arg1	sulfide					511:517	copper sulfide	504:517	copper sulfide (CuS)	504:523	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	4	118	theme	CuS	740:742	arg1	dots					744:747	the CuS dots	736:747	the CuS dots	736:747	S-S bonds connect the CuS dots to the HMSNs; these are selectively cleaved under the reducing microenvironment of the tumor, permitting targeted drug release.					
33731303	13	119	theme	nanoparticles	2082:2094	arg1	cavity					2024:2029	the central cavity	2012:2029	the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs)	2012:2102	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	2	120	dep	chitosan	486:493	arg1	nanodots					525:532	nanodots	525:532	nanodots	525:532	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	16	121	theme	systematic	2349:2358	arg1	evaluation					2381:2390	a systematic in vitro and in vivo evaluation	2347:2390	a systematic in vitro and in vivo evaluation	2347:2390	In addition, a systematic in vitro and in vivo evaluation confirmed the HMSNs-CS-DOX@CuS platform to give highly effective synergistic chemotherapeutic-photothermal therapy and have effective thermal/photoacoustic dual-imaging properties.					
33731303	0	122	dep	Nanoparticles	25:37	arg1	Gated					39:43	Gated	39:43	Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer	25:138	Hollow Mesoporous Silica Nanoparticles Gated by Chitosan-Copper Sulfide Composites as Theranostic Agents for the Treatment of Breast Cancer.					
33731303	6	123	theme	uniform	1005:1011	arg1	nm					1028:1029	150 ± 13 nm	1019:1029	150 ± 13 nm	1019:1029	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	6	123	theme	uniform	1005:1011	arg1	size					1013:1016	a uniform size	1003:1016	a uniform size (150 ± 13 nm)	1003:1030	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	2	124	theme	silica	335:340	arg1	surface					458:464	surface	458:464	surface	458:464	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	124	theme	silica	335:340	arg1	material					392:399	a base carrier material	377:399	a base carrier material	377:399	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	124	theme	silica	335:340	arg1	HMSNs					357:361	HMSNs	357:361	HMSNs	357:361	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	2	124	theme	silica	335:340	arg1	nanoparticles					342:354	hollow mesoporous silica nanoparticles	317:354	hollow mesoporous silica nanoparticles (HMSNs)	317:362	Here, hollow mesoporous silica nanoparticles (HMSNs) were used as a base carrier material, loaded with the anti-cancer drug doxorubicin (DOX), and surface functionalized with chitosan (CS) and copper sulfide (CuS) nanodots to give HMSNs-CS-DOX@CuS.					
33731303	15	125	theme	near	2196:2199	arg1	irradiation					2222:2232	near infrared laser (NIR) irradiation	2196:2232	near infrared laser (NIR) irradiation	2196:2232	When exposed to near infrared laser (NIR) irradiation, CuS nanodots located at the surface pores of the HMSNs generate energy, accelerating drug release.					
33731303	6	126	dep	tumor-targeted	1082:1095	arg1	infrared					1106:1113	infrared	1106:1113	infrared	1106:1113	The HMSNs-CS-DOX@CuS nanoparticles have a uniform size (150 ± 13 nm), potent photothermal properties (η = 36.4 %), and tumor-targeted and near infrared (NIR) laser irradiation-triggered DOX release.					
33731303	5	127	theme	PTT	900:902	arg1	properties					904:913	the PTT properties	896:913	the PTT properties of CuS	896:920	This, coupled with the PTT properties of CuS, results in a potent chemo/PTT platform.					
33731303	11	128	theme	Conventional	1768:1779	arg1	chemotherapy					1788:1799	Conventional cancer chemotherapy	1768:1799	Conventional cancer chemotherapy	1768:1799	STATEMENT OF SIGNIFICANCE: Conventional cancer chemotherapy is accompanied by unavoidable off-target toxicity.					
33731303	4	129	theme	S-S	718:720	arg1	bonds					722:726	S-S bonds	718:726	S-S bonds	718:726	S-S bonds connect the CuS dots to the HMSNs; these are selectively cleaved under the reducing microenvironment of the tumor, permitting targeted drug release.					
33731303	13	130	theme	-modified	2047:2055	arg1	HMSNs					2097:2101	HMSNs	2097:2101	HMSNs	2097:2101	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33731303	13	130	theme	-modified	2047:2055	arg1	nanoparticles					2082:2094	hollow mesoporous silica nanoparticles	2057:2094	chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs)	2034:2102	Here, the anticancer drug doxorubicin (DOX) was encapsulated in the central cavity of chitosan (CS)-modified hollow mesoporous silica nanoparticles (HMSNs).					
33814020	0	0	theme	microRNA	81:88	arg1	levels					90:95	select maternal milk microRNA levels	60:95	select maternal milk microRNA levels	60:95	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	3	1	theme	HFS	667:669	arg1	HFS-WM					684:689	HFS-WM	684:689	HFS-WM	684:689	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	3	1	theme	HFS	667:669	arg1	group					677:681	the HFS + OFS group	663:681	group	677:681	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	11	2	theme	milk	1494:1497	arg1	miRNA					1499:1503	milk protein and multiple milk miRNA and offspring body composition	1468:1534	miRNA	1499:1503	Correlations between milk protein and multiple milk miRNA and offspring body composition and gut microbiota differed by sex.					
33814020	10	3	from	Enterobacteriaceae	1388:1405	arg1	offspring					1417:1425	female offspring	1410:1425	female offspring	1410:1425	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	0	4	theme	milk	76:79	arg1	levels					90:95	select maternal milk microRNA levels	60:95	select maternal milk microRNA levels	60:95	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	3	5	theme	weight-matched	645:658	arg1	diet					640:643	HFS diet	636:643	HFS diet weight-matched to the HFS + OFS group (HFS-WM)	636:690	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	2	6	with	rats	485:488	arg1	microbiota					547:556	gut microbiota	543:556	gut microbiota	543:556	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	2	6	with	rats	485:488	arg1	composition					527:537	offspring body composition	512:537	offspring body composition	512:537	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	8	7	theme	male	1205:1208	arg1	offspring					1210:1218	male offspring	1205:1218	male offspring	1205:1218	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	9	8	theme	maternal	1291:1298	arg1	fat					1305:1307	maternal body fat	1291:1307	maternal body fat	1291:1307	Milk protein content was inversely associated with maternal body fat and body weight.					
33814020	0	9	from	Impact	0:5	arg1	correlation					101:111	correlation	101:111	correlation with offspring outcomes	101:135	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	0	9	from	Impact	0:5	arg1	levels					90:95	select maternal milk microRNA levels	60:95	select maternal milk microRNA levels	60:95	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	12	10	theme	maternal	1786:1793	arg1	diets					1795:1799	these maternal diets	1780:1799	these maternal diets	1780:1799	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	8	11	from	fat	1168:1170	arg1	offspring					1210:1218	male offspring	1205:1218	male offspring	1205:1218	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	12	12	theme	miRNA	1679:1683	arg1	levels					1685:1690	the protein and miRNA levels	1663:1690	levels	1685:1690	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	9	13	theme	body	1300:1303	arg1	fat					1305:1307	maternal body fat	1291:1307	maternal body fat	1291:1307	Milk protein content was inversely associated with maternal body fat and body weight.					
33814020	2	14	theme	body	522:525	arg1	composition					527:537	offspring body composition	512:537	offspring body composition	512:537	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	12	15	theme	diets	1795:1799	arg1	influence					1767:1775	the influence	1763:1775	the influence of these maternal diets on offspring body composition	1763:1829	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	2	16	with	associations	494:505	arg1	microbiota					547:556	gut microbiota	543:556	gut microbiota	543:556	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	2	16	with	associations	494:505	arg1	composition					527:537	offspring body composition	512:537	offspring body composition	512:537	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	10	17	theme	body	1375:1378	arg1	fat					1380:1382	maternal body fat	1366:1382	maternal body fat	1366:1382	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	2	18	theme	offspring	512:520	arg1	composition					527:537	offspring body composition	512:537	offspring body composition	512:537	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	11	19	theme	gut	1540:1542	arg1	microbiota					1544:1553	gut microbiota	1540:1553	gut microbiota	1540:1553	Correlations between milk protein and multiple milk miRNA and offspring body composition and gut microbiota differed by sex.					
33814020	1	20	theme	maternal	174:181	arg1	diet					201:204	diet	201:204	diet	201:204	Breast milk composition varies with maternal factors including diet and confers health benefits to the neonate; however, the mechanisms mediating this protection remain incompletely understood.					
33814020	1	20	theme	maternal	174:181	arg1	factors					183:189	maternal factors	174:189	maternal factors including diet	174:204	Breast milk composition varies with maternal factors including diet and confers health benefits to the neonate; however, the mechanisms mediating this protection remain incompletely understood.					
33814020	2	21	from	composition	470:480	arg1	associations					494:505	associations	494:505	associations with offspring body composition and gut microbiota	494:556	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	2	21	from	composition	470:480	arg1	rats					485:488	rats	485:488	rats	485:488	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	7	22	dep	miRNA	971:975	arg1	miR-200a					997:1004	miR-200a	997:1004	miR-200a	997:1004	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	7	22	dep	miRNA	971:975	arg1	miR-103					1028:1034	miR-103	1028:1034	miR-103	1028:1034	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	7	22	dep	miRNA	971:975	arg1	miRNA					971:975	Six miRNA	967:975	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103)	967:1035	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	7	22	dep	miRNA	971:975	arg1	miR-222					978:984	miR-222	978:984	miR-222	978:984	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	7	22	dep	miRNA	971:975	arg1	miR-26a					1007:1013	miR-26a	1007:1013	miR-26a	1007:1013	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	7	22	dep	miRNA	971:975	arg1	miR-27a					1016:1022	miR-27a	1016:1022	miR-27a	1016:1022	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	7	22	dep	miRNA	971:975	arg1	miR-203a					987:994	miR-203a	987:994	miR-203a	987:994	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	2	23	from	effects	363:369	arg1	composition					470:480	milk composition	465:480	milk composition in rats and associations with offspring body composition and gut microbiota	465:556	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	2	24	theme	high-fat/sucrose	399:414	arg1	diet					422:425	a maternal high-fat/sucrose (HFS) diet	388:425	a maternal high-fat/sucrose (HFS) diet	388:425	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	3	25	theme	OFS	673:675	arg1	HFS-WM					684:689	HFS-WM	684:689	HFS-WM	684:689	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	3	25	theme	OFS	673:675	arg1	group					677:681	the HFS + OFS group	663:681	group	677:681	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	7	26	theme	maternal	1088:1095	arg1	diet					1097:1100	maternal diet	1088:1100	maternal diet	1088:1100	Six miRNA (miR-222, miR-203a, miR-200a, miR-26a, miR-27a and miR-103) were differentially expressed in milk according to maternal diet.					
33814020	0	27	with	correlation	101:111	arg1	outcomes					128:135	offspring outcomes	118:135	offspring outcomes	118:135	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	5	28	theme	miRNA	845:849	arg1	levels					852:857	microRNA (miRNA) levels	835:857	microRNA (miRNA) levels	835:857	Milk was collected at weaning and analysed for protein, leptin and microRNA (miRNA) levels.					
33814020	2	29	theme	maternal	390:397	arg1	diet					422:425	a maternal high-fat/sucrose (HFS) diet	388:425	a maternal high-fat/sucrose (HFS) diet	388:425	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	10	30	theme	maternal	1366:1373	arg1	fat					1380:1382	maternal body fat	1366:1382	maternal body fat	1366:1382	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	6	31	theme	HFS	877:879	arg1	dams					881:884	HFS dams	877:884	HFS dams	877:884	Milk produced by HFS dams contained less protein than milk from lean controls which was normalised by OFS.					
33814020	8	32	theme	leptin	1108:1113	arg1	content					1115:1121	Milk leptin content	1103:1121	Milk leptin content	1103:1121	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	12	33	theme	prebiotic	1627:1635	arg1	supplementation					1637:1651	prebiotic supplementation	1627:1651	prebiotic supplementation	1627:1651	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	6	34	contain	contained	886:894	arg2	protein					901:907	less protein	896:907	less protein	896:907	Milk produced by HFS dams contained less protein than milk from lean controls which was normalised by OFS.					
33814020	6	34	contain	contained	886:894	arg1	Milk					860:863	Milk	860:863	Milk produced by HFS dams	860:884	Milk produced by HFS dams contained less protein than milk from lean controls which was normalised by OFS.					
33814020	11	35	theme	offspring	1509:1517	arg1	composition					1524:1534	milk protein and multiple milk miRNA and offspring body composition	1468:1534	composition	1524:1534	Correlations between milk protein and multiple milk miRNA and offspring body composition and gut microbiota differed by sex.					
33814020	0	36	theme	obesity	19:25	arg1	Impact					0:5	Impact	0:5	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.	0:136	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	0	37	with	levels	90:95	arg1	outcomes					128:135	offspring outcomes	118:135	offspring outcomes	118:135	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	3	38	theme	Obese	559:563	arg1	dams					580:583	Obese Sprague-Dawley dams	559:583	Obese Sprague-Dawley dams	559:583	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	0	39	theme	maternal	10:17	arg1	obesity					19:25	maternal obesity	10:25	maternal obesity	10:25	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	0	40	theme	offspring	118:126	arg1	outcomes					128:135	offspring outcomes	118:135	offspring outcomes	118:135	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	3	41	theme	%	624:624	arg1	HFS					605:607	HFS	605:607	HFS	605:607	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	3	41	theme	%	624:624	arg1	wt/wt					626:630	10 % wt/wt	621:630	10 % wt/wt	621:630	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	3	42	theme	Sprague-Dawley	565:578	arg1	dams					580:583	Obese Sprague-Dawley dams	559:583	Obese Sprague-Dawley dams	559:583	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	12	43	from	levels	1685:1690	arg1	milk					1702:1705	breast milk	1695:1705	breast milk in rats	1695:1713	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	0	44	theme	prebiotic	31:39	arg1	supplementation					41:55	prebiotic supplementation	31:55	prebiotic supplementation	31:55	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	12	45	from	milk	1702:1705	arg1	rats					1710:1713	rats	1710:1713	rats	1710:1713	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	2	46	theme	gut	543:545	arg1	microbiota					547:556	gut microbiota	543:556	gut microbiota	543:556	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	8	47	theme	maternal	1154:1161	arg1	fat					1168:1170	maternal body fat	1154:1170	maternal body fat	1154:1170	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	1	48	theme	health	218:223	arg1	benefits					225:232	health benefits	218:232	health benefits	218:232	Breast milk composition varies with maternal factors including diet and confers health benefits to the neonate; however, the mechanisms mediating this protection remain incompletely understood.					
33814020	2	49	theme	milk	465:468	arg1	composition					470:480	milk composition	465:480	milk composition in rats and associations with offspring body composition and gut microbiota	465:556	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	9	50	theme	protein	1245:1251	arg1	content					1253:1259	Milk protein content	1240:1259	Milk protein content	1240:1259	Milk protein content was inversely associated with maternal body fat and body weight.					
33814020	10	51	from	weeks	1433:1437	arg1	Enterobacteriaceae					1388:1405	Enterobacteriaceae	1388:1405	Enterobacteriaceae	1388:1405	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	10	51	from	weeks	1433:1437	arg1	fat					1380:1382	maternal body fat	1366:1382	maternal body fat	1366:1382	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	4	52	theme	HFS	748:750	arg1	diet					752:755	a HFS diet	746:755	a HFS diet	746:755	Pups were weaned onto a HFS diet on day 21.					
33814020	12	53	theme	breast	1695:1700	arg1	milk					1702:1705	breast milk	1695:1705	breast milk in rats	1695:1713	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	2	54	theme	HFS	417:419	arg1	diet					422:425	a maternal high-fat/sucrose (HFS) diet	388:425	a maternal high-fat/sucrose (HFS) diet	388:425	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	12	55	theme	body	1814:1817	arg1	composition					1819:1829	body composition	1814:1829	body composition	1814:1829	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	9	56	theme	body	1313:1316	arg1	weight					1318:1323	body weight	1313:1323	body weight	1313:1323	Milk protein content was inversely associated with maternal body fat and body weight.					
33814020	10	57	theme	female	1410:1415	arg1	offspring					1417:1425	female offspring	1410:1425	female offspring	1410:1425	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	8	58	theme	age	1235:1237	arg1	weeks					1226:1230	24 weeks	1223:1230	24 weeks of age	1223:1237	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	11	59	theme	body	1519:1522	arg1	composition					1524:1534	milk protein and multiple milk miRNA and offspring body composition	1468:1534	composition	1524:1534	Correlations between milk protein and multiple milk miRNA and offspring body composition and gut microbiota differed by sex.					
33814020	0	60	theme	supplementation	41:55	arg1	Impact					0:5	Impact	0:5	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.	0:136	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	9	61	theme	Milk	1240:1243	arg1	content					1253:1259	Milk protein content	1240:1259	Milk protein content	1240:1259	Milk protein content was inversely associated with maternal body fat and body weight.					
33814020	12	62	theme	obesogenic	1606:1615	arg1	diets					1617:1621	obesogenic diets	1606:1621	obesogenic diets	1606:1621	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	3	63	theme	HFS	636:638	arg1	diet					640:643	HFS diet	636:643	HFS diet weight-matched to the HFS + OFS group (HFS-WM)	636:690	Obese Sprague-Dawley dams consumed a control, HFS, HFS + OFS (10 % wt/wt) or HFS diet weight-matched to the HFS + OFS group (HFS-WM) during pregnancy and lactation.					
33814020	8	64	theme	body	1163:1166	arg1	fat					1168:1170	maternal body fat	1154:1170	maternal body fat	1154:1170	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	8	65	from	Enterobacteriaceae	1183:1200	arg1	offspring					1210:1218	male offspring	1205:1218	male offspring	1205:1218	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	12	66	theme	milk	1725:1728	arg1	components					1730:1739	these milk components	1719:1739	these milk components	1719:1739	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	6	67	from	controls	929:936	arg1	milk					914:917	milk	914:917	milk from lean controls which was normalised by OFS	914:964	Milk produced by HFS dams contained less protein than milk from lean controls which was normalised by OFS.					
33814020	11	68	theme	milk	1468:1471	arg1	protein					1473:1479	milk protein and multiple milk miRNA and offspring body composition	1468:1534	protein	1473:1479	Correlations between milk protein and multiple milk miRNA and offspring body composition and gut microbiota differed by sex.					
33814020	5	69	theme	microRNA	835:842	arg1	levels					852:857	microRNA (miRNA) levels	835:857	microRNA (miRNA) levels	835:857	Milk was collected at weaning and analysed for protein, leptin and microRNA (miRNA) levels.					
33814020	8	70	from	weeks	1226:1230	arg1	fat					1168:1170	maternal body fat	1154:1170	maternal body fat	1154:1170	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	8	70	from	weeks	1226:1230	arg1	Enterobacteriaceae					1183:1200	faecal Enterobacteriaceae	1176:1200	faecal Enterobacteriaceae	1176:1200	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	6	71	theme	lean	924:927	arg1	controls					929:936	lean controls	924:936	lean controls which was normalised by OFS	924:964	Milk produced by HFS dams contained less protein than milk from lean controls which was normalised by OFS.					
33814020	10	72	theme	age	1442:1444	arg1	weeks					1433:1437	24 weeks	1430:1437	24 weeks of age	1430:1444	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	1	73	theme	Breast	138:143	arg1	composition					150:160	Breast milk composition	138:160	Breast milk composition	138:160	Breast milk composition varies with maternal factors including diet and confers health benefits to the neonate; however, the mechanisms mediating this protection remain incompletely understood.					
33814020	8	74	theme	faecal	1176:1181	arg1	Enterobacteriaceae					1183:1200	faecal Enterobacteriaceae	1176:1200	faecal Enterobacteriaceae	1176:1200	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	0	75	theme	maternal	67:74	arg1	levels					90:95	select maternal milk microRNA levels	60:95	select maternal milk microRNA levels	60:95	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	12	76	theme	protein	1667:1673	arg1	levels					1685:1690	the protein and miRNA levels	1663:1690	levels	1685:1690	Overall, our results suggest that obesogenic diets and prebiotic supplementation can alter the protein and miRNA levels in breast milk in rats and these milk components may explain, in part, the influence of these maternal diets on offspring body composition.					
33814020	1	77	theme	milk	145:148	arg1	composition					150:160	Breast milk composition	138:160	Breast milk composition	138:160	Breast milk composition varies with maternal factors including diet and confers health benefits to the neonate; however, the mechanisms mediating this protection remain incompletely understood.					
33814020	8	78	theme	Milk	1103:1106	arg1	content					1115:1121	Milk leptin content	1103:1121	Milk leptin content	1103:1121	Milk leptin content was positively correlated with maternal body fat and faecal Enterobacteriaceae in male offspring at 24 weeks of age.					
33814020	10	79	from	fat	1380:1382	arg1	offspring					1417:1425	female offspring	1410:1425	female offspring	1410:1425	miR-200a was positively associated with maternal body fat and Enterobacteriaceae in female offspring at 24 weeks of age.					
33814020	0	80	theme	select	60:65	arg1	levels					90:95	select maternal milk microRNA levels	60:95	select maternal milk microRNA levels	60:95	Impact of maternal obesity and prebiotic supplementation on select maternal milk microRNA levels and correlation with offspring outcomes.					
33814020	2	81	theme	prebiotic	432:440	arg1	OFS					457:459	OFS	457:459	OFS	457:459	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	2	81	theme	prebiotic	432:440	arg1	oligofructose					442:454	prebiotic oligofructose	432:454	prebiotic oligofructose (OFS)	432:460	Our aim was to investigate the effects of supplementing a maternal high-fat/sucrose (HFS) diet with prebiotic oligofructose (OFS) on milk composition in rats and associations with offspring body composition and gut microbiota.					
33814020	11	82	theme	multiple	1485:1492	arg1	miRNA					1499:1503	milk protein and multiple milk miRNA and offspring body composition	1468:1534	miRNA	1499:1503	Correlations between milk protein and multiple milk miRNA and offspring body composition and gut microbiota differed by sex.					
33838351	12	0	from	role	1492:1495	arg1	medicine					1532:1539	regenerative medicine	1519:1539	regenerative medicine	1519:1539	CONCLUSION The results demonstrated the potential role of these particles in regenerative medicine to improve angiogenesis.					
33838351	12	1	theme	potential	1482:1490	arg1	role					1492:1495	the potential role	1478:1495	the potential role of these particles in regenerative medicine	1478:1539	CONCLUSION The results demonstrated the potential role of these particles in regenerative medicine to improve angiogenesis.					
33838351	4	2	theme	alginate	616:623	arg1	oxidation					596:604	the oxidation	592:604	the oxidation of sodium alginate	592:623	METHODS The particles were prepared by the oxidation of sodium alginate.					
33838351	5	3	theme	collagen-alginate	654:670	arg1	hydrogel					672:679	collagen-alginate hydrogel	654:679	collagen-alginate hydrogel	654:679	Then, they were embedded in collagen-alginate hydrogel.					
33838351	2	4	theme	factors	287:293	arg1	delivery					268:275	The delivery	264:275	The delivery of growth factors with nanocarriers	264:311	The delivery of growth factors with nanocarriers can eliminate these problems.					
33838351	7	5	theme	VEGF	861:864	arg1	evaluation					847:856	In vitro evaluation	838:856	In vitro evaluation of VEGF and bFGF Release Kinetics	838:890	In vitro evaluation of VEGF and bFGF Release Kinetics was done.					
33838351	11	6	theme	=	1406:1406	arg1	group					1397:1401	the control group	1385:1401	the control group (p = 0.010)	1385:1413	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	11	6	theme	=	1406:1406	arg1	0.010					1408:1412	p = 0.010	1404:1412	p = 0.010	1404:1412	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	10	7	from	Points	1237:1242	arg1	assay					1282:1286	the chick chorioallantoic membrane assay	1247:1286	the chick chorioallantoic membrane assay	1247:1286	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	7	8	theme	bFGF	870:873	arg1	evaluation					847:856	In vitro evaluation	838:856	In vitro evaluation of VEGF and bFGF Release Kinetics	838:890	In vitro evaluation of VEGF and bFGF Release Kinetics was done.					
33838351	9	9	theme	umbilical	1062:1070	arg1	cells					1089:1093	human umbilical vein endothelial cells	1056:1093	the human umbilical vein endothelial cells viability	1052:1103	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	9	10	theme	endothelial	1077:1087	arg1	cells					1089:1093	human umbilical vein endothelial cells	1056:1093	the human umbilical vein endothelial cells viability	1052:1103	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	2	11	with	factors	287:293	arg1	nanocarriers					300:311	nanocarriers	300:311	nanocarriers	300:311	The delivery of growth factors with nanocarriers can eliminate these problems.					
33838351	3	12	theme	present	350:356	arg1	study					358:362	the present study	346:362	the present study	346:362	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	0	13	theme	acellular	66:74	arg1	hydrogel					104:111	acellular collagen-alginate composite hydrogel	66:111	acellular collagen-alginate composite hydrogel to promote angiogenesis	66:135	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	5	14	from	embedded	642:649	arg1	hydrogel					672:679	collagen-alginate hydrogel	654:679	collagen-alginate hydrogel	654:679	Then, they were embedded in collagen-alginate hydrogel.					
33838351	11	15	theme	VEGF	1362:1365	arg1	group					1367:1371	the VEGF group	1358:1371	the VEGF group	1358:1371	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	0	16	theme	composite	94:102	arg1	hydrogel					104:111	acellular collagen-alginate composite hydrogel	66:111	acellular collagen-alginate composite hydrogel to promote angiogenesis	66:135	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	1	17	theme	tissue	178:183	arg1	engineering					185:195	tissue engineering	178:195	tissue engineering	178:195	BACKGROUND The use of growth factors in tissue engineering is often challenging due to their instability and short half-life.					
33838351	0	18	from	Incorporation	0:12	arg1	hydrogel					104:111	acellular collagen-alginate composite hydrogel	66:111	acellular collagen-alginate composite hydrogel to promote angiogenesis	66:135	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	10	19	theme	VEGF-loaded	1181:1191	arg1	particles					1193:1201	VEGF-loaded particles	1181:1201	VEGF-loaded particles	1181:1201	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	1	20	dep	BACKGROUND	138:147	arg1	challenging					206:216	challenging	206:216	is often challenging due to their instability and short half-life	197:261	BACKGROUND The use of growth factors in tissue engineering is often challenging due to their instability and short half-life.					
33838351	9	21	theme	tested	1146:1151	arg1	constructs					1153:1162	the tested constructs	1142:1162	the tested constructs	1142:1162	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	3	22	from	particle	397:404	arg1	platform					456:463	acellular collagen-alginate composite hydrogel platform	409:463	acellular collagen-alginate composite hydrogel platform for the immobilization	409:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	1	23	from	use	153:155	arg1	engineering					185:195	tissue engineering	178:195	tissue engineering	178:195	BACKGROUND The use of growth factors in tissue engineering is often challenging due to their instability and short half-life.					
33838351	6	24	theme	vein	742:745	arg1	cells					759:763	the human umbilical vein endothelial cells	722:763	the human umbilical vein endothelial cells	722:763	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	5	25	from	hydrogel	672:679	arg1	embedded					642:649	embedded	642:649	embedded	642:649	Then, they were embedded in collagen-alginate hydrogel.					
33838351	3	26	theme	alginate	382:389	arg1	particle					397:404	an alginate oxide particle	379:404	an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization	379:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	11	27	theme	Total	1305:1309	arg1	Points					1321:1326	Total Branching Points	1305:1326	Total Branching Points	1305:1326	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	6	28	theme	human	726:730	arg1	cells					759:763	the human umbilical vein endothelial cells	722:763	the human umbilical vein endothelial cells	722:763	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	0	29	theme	VEGF-and	17:24	arg1	particles					53:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	3	30	theme	composite	437:445	arg1	platform					456:463	acellular collagen-alginate composite hydrogel platform	409:463	acellular collagen-alginate composite hydrogel platform for the immobilization	409:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	0	31	theme	alginate	38:45	arg1	particles					53:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	4	32	dep	METHODS	553:559	arg1	particles					565:573	The particles	561:573	METHODS The particles	553:573	METHODS The particles were prepared by the oxidation of sodium alginate.					
33838351	8	33	theme	chick	969:973	arg1	assay					1000:1004	the chick chorioallantoic membrane assay	965:1004	the chick chorioallantoic membrane assay	965:1004	Moreover, the function of the constructs was confirmed through the chick chorioallantoic membrane assay.					
33838351	3	34	theme	acellular	409:417	arg1	platform					456:463	acellular collagen-alginate composite hydrogel platform	409:463	acellular collagen-alginate composite hydrogel platform for the immobilization	409:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	7	35	theme	In	838:839	arg1	evaluation					847:856	In vitro evaluation	838:856	In vitro evaluation of VEGF and bFGF Release Kinetics	838:890	In vitro evaluation of VEGF and bFGF Release Kinetics was done.					
33838351	6	36	theme	scanning	808:815	arg1	microscopy					826:835	scanning electron microscopy	808:835	scanning electron microscopy	808:835	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	3	37	theme	bFGF	523:526	arg1	particle					397:404	an alginate oxide particle	379:404	an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization	379:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	3	37	theme	bFGF	523:526	arg1	release					503:509	controlled release	492:509	controlled release of VEGF and bFGF	492:526	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	0	38	theme	particles	53:61	arg1	Incorporation					0:12	Incorporation	0:12	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.	0:136	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	10	39	theme	Branching	1227:1235	arg1	Points					1237:1242	the Total Branching Points	1217:1242	the Total Branching Points in the chick chorioallantoic membrane assay	1217:1286	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	8	40	theme	membrane	991:998	arg1	assay					1000:1004	the chick chorioallantoic membrane assay	965:1004	the chick chorioallantoic membrane assay	965:1004	Moreover, the function of the constructs was confirmed through the chick chorioallantoic membrane assay.					
33838351	3	41	theme	VEGF	514:517	arg1	particle					397:404	an alginate oxide particle	379:404	an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization	379:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	3	41	theme	VEGF	514:517	arg1	release					503:509	controlled release	492:509	controlled release of VEGF and bFGF	492:526	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	10	42	theme	membrane	1273:1280	arg1	assay					1282:1286	the chick chorioallantoic membrane assay	1247:1286	the chick chorioallantoic membrane assay	1247:1286	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	1	43	theme	factors	167:173	arg1	use					153:155	The use	149:155	The use of growth factors in tissue engineering	149:195	BACKGROUND The use of growth factors in tissue engineering is often challenging due to their instability and short half-life.					
33838351	11	44	theme	p	1430:1430	arg1	group					1423:1427	FGF group	1419:1427	FGF group (p = 0.023)	1419:1439	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	11	44	theme	p	1430:1430	arg1	0.023					1434:1438	p = 0.023	1430:1438	p = 0.023	1430:1438	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	12	45	dep	CONCLUSION	1442:1451	arg1	demonstrated					1465:1476	demonstrated	1465:1476	demonstrated the potential role of these particles in regenerative medicine to improve angiogenesis	1465:1563	CONCLUSION The results demonstrated the potential role of these particles in regenerative medicine to improve angiogenesis.					
33838351	10	46	theme	chick	1251:1255	arg1	assay					1282:1286	the chick chorioallantoic membrane assay	1247:1286	the chick chorioallantoic membrane assay	1247:1286	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	11	47	theme	p	1404:1404	arg1	group					1397:1401	the control group	1385:1401	the control group (p = 0.010)	1385:1413	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	11	47	theme	p	1404:1404	arg1	0.010					1408:1412	p = 0.010	1404:1412	p = 0.010	1404:1412	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	7	48	dep	VEGF	861:864	arg1	Kinetics					883:890	Release Kinetics	875:890	Release Kinetics	875:890	In vitro evaluation of VEGF and bFGF Release Kinetics was done.					
33838351	4	49	theme	sodium	609:614	arg1	alginate					616:623	sodium alginate	609:623	sodium alginate	609:623	METHODS The particles were prepared by the oxidation of sodium alginate.					
33838351	7	50	dep	In	838:839	arg1	vitro					841:845	vitro	841:845	vitro	841:845	In vitro evaluation of VEGF and bFGF Release Kinetics was done.					
33838351	9	51	theme	engineered	1019:1028	arg1	constructs					1030:1039	The engineered constructs	1015:1039	The engineered constructs	1015:1039	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	9	52	theme	human	1056:1060	arg1	cells					1089:1093	human umbilical vein endothelial cells	1056:1093	the human umbilical vein endothelial cells viability	1052:1103	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	7	53	theme	Release	875:881	arg1	Kinetics					883:890	Release Kinetics	875:890	Release Kinetics	875:890	In vitro evaluation of VEGF and bFGF Release Kinetics was done.					
33838351	9	54	theme	vein	1072:1075	arg1	cells					1089:1093	human umbilical vein endothelial cells	1056:1093	the human umbilical vein endothelial cells viability	1052:1103	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	9	55	theme	cells	1089:1093	arg1	viability					1095:1103	the human umbilical vein endothelial cells viability	1052:1103	the human umbilical vein endothelial cells viability	1052:1103	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	2	56	theme	growth	280:285	arg1	factors					287:293	growth factors	280:293	growth factors with nanocarriers	280:311	The delivery of growth factors with nanocarriers can eliminate these problems.					
33838351	0	57	theme	collagen-alginate	76:92	arg1	hydrogel					104:111	acellular collagen-alginate composite hydrogel	66:111	acellular collagen-alginate composite hydrogel to promote angiogenesis	66:135	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	1	58	theme	growth	160:165	arg1	factors					167:173	growth factors	160:173	growth factors	160:173	BACKGROUND The use of growth factors in tissue engineering is often challenging due to their instability and short half-life.					
33838351	6	59	with	Cytocompatibility	682:698	arg1	cells					759:763	the human umbilical vein endothelial cells	722:763	the human umbilical vein endothelial cells	722:763	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	11	60	theme	control	1389:1395	arg1	group					1397:1401	the control group	1385:1401	the control group (p = 0.010)	1385:1413	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	11	60	theme	control	1389:1395	arg1	0.010					1408:1412	p = 0.010	1404:1412	p = 0.010	1404:1412	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	1	61	theme	short	247:251	arg1	half-life					253:261	short half-life	247:261	short half-life	247:261	BACKGROUND The use of growth factors in tissue engineering is often challenging due to their instability and short half-life.					
33838351	6	62	theme	endothelial	747:757	arg1	cells					759:763	the human umbilical vein endothelial cells	722:763	the human umbilical vein endothelial cells	722:763	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	6	63	theme	construct	707:715	arg1	Cytocompatibility					682:698	Cytocompatibility	682:698	Cytocompatibility of the construct with the human umbilical vein endothelial cells	682:763	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	0	64	dep	hydrogel	104:111	arg1	promote					116:122	promote	116:122	to promote angiogenesis	113:135	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	9	65	theme	constructs	1153:1162	arg1	non-toxicity					1126:1137	the non-toxicity	1122:1137	the non-toxicity of the tested constructs	1122:1162	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	6	66	theme	umbilical	732:740	arg1	cells					759:763	the human umbilical vein endothelial cells	722:763	the human umbilical vein endothelial cells	722:763	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	0	67	theme	bFGF-loaded	26:36	arg1	particles					53:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	8	68	theme	constructs	932:941	arg1	function					916:923	the function	912:923	the function of the constructs	912:941	Moreover, the function of the constructs was confirmed through the chick chorioallantoic membrane assay.					
33838351	3	69	theme	oxide	391:395	arg1	particle					397:404	an alginate oxide particle	379:404	an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization	379:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	11	70	theme	Branching	1311:1319	arg1	Points					1321:1326	Total Branching Points	1305:1326	Total Branching Points	1305:1326	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	0	71	theme	oxide	47:51	arg1	particles					53:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	VEGF-and bFGF-loaded alginate oxide particles	17:61	Incorporation of VEGF-and bFGF-loaded alginate oxide particles in acellular collagen-alginate composite hydrogel to promote angiogenesis.					
33838351	10	72	theme	particles	1193:1201	arg1	presence					1169:1176	The presence	1165:1176	The presence of VEGF-loaded particles	1165:1201	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	3	73	theme	hydrogel	447:454	arg1	platform					456:463	acellular collagen-alginate composite hydrogel platform	409:463	acellular collagen-alginate composite hydrogel platform for the immobilization	409:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	11	74	theme	FGF	1419:1421	arg1	group					1423:1427	FGF group	1419:1427	FGF group (p = 0.023)	1419:1439	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	11	74	theme	FGF	1419:1421	arg1	0.023					1434:1438	p = 0.023	1430:1438	p = 0.023	1430:1438	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	6	75	theme	electron	817:824	arg1	microscopy					826:835	scanning electron microscopy	808:835	scanning electron microscopy	808:835	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	3	76	theme	controlled	492:501	arg1	release					503:509	controlled release	492:509	controlled release of VEGF and bFGF	492:526	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	9	77	dep	RESULTS	1007:1013	arg1	maintained					1041:1050	maintained	1041:1050	maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs	1041:1162	RESULTS The engineered constructs maintained the human umbilical vein endothelial cells viability, which indicates the non-toxicity of the tested constructs.					
33838351	3	78	theme	collagen-alginate	419:435	arg1	platform					456:463	acellular collagen-alginate composite hydrogel platform	409:463	acellular collagen-alginate composite hydrogel platform for the immobilization	409:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	3	79	from	release	503:509	arg1	platform					456:463	acellular collagen-alginate composite hydrogel platform	409:463	acellular collagen-alginate composite hydrogel platform for the immobilization	409:486	In the present study, we introduced an alginate oxide particle in acellular collagen-alginate composite hydrogel platform for the immobilization and controlled release of VEGF and bFGF to promote angiogenesis.					
33838351	10	80	theme	Total	1221:1225	arg1	Points					1237:1242	the Total Branching Points	1217:1242	the Total Branching Points in the chick chorioallantoic membrane assay	1217:1286	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	12	81	theme	particles	1506:1514	arg1	role					1492:1495	the potential role	1478:1495	the potential role of these particles in regenerative medicine	1478:1539	CONCLUSION The results demonstrated the potential role of these particles in regenerative medicine to improve angiogenesis.					
33838351	8	82	theme	chorioallantoic	975:989	arg1	assay					1000:1004	the chick chorioallantoic membrane assay	965:1004	the chick chorioallantoic membrane assay	965:1004	Moreover, the function of the constructs was confirmed through the chick chorioallantoic membrane assay.					
33838351	6	83	theme	live/dead	788:796	arg1	assay					798:802	a live/dead assay	786:802	a live/dead assay	786:802	Cytocompatibility of the construct with the human umbilical vein endothelial cells was analyzed through a live/dead assay and scanning electron microscopy.					
33838351	10	84	theme	chorioallantoic	1257:1271	arg1	assay					1282:1286	the chick chorioallantoic membrane assay	1247:1286	the chick chorioallantoic membrane assay	1247:1286	The presence of VEGF-loaded particles could improve the Total Branching Points in the chick chorioallantoic membrane assay.					
33838351	12	85	theme	regenerative	1519:1530	arg1	medicine					1532:1539	regenerative medicine	1519:1539	regenerative medicine	1519:1539	CONCLUSION The results demonstrated the potential role of these particles in regenerative medicine to improve angiogenesis.					
33838351	11	86	theme	=	1432:1432	arg1	group					1423:1427	FGF group	1419:1427	FGF group (p = 0.023)	1419:1439	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
33838351	11	86	theme	=	1432:1432	arg1	0.023					1434:1438	p = 0.023	1430:1438	p = 0.023	1430:1438	In this regard, Total Branching Points was significantly improved in the VEGF group compared to the control group (p = 0.010) and FGF group (p = 0.023).					
32871299	3	0	from	albumin	624:630	arg1	surfaces					641:648	their surfaces	635:648	their surfaces	635:648	Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together.					
32871299	2	1	theme	excellent	547:555	arg1	species					462:468	a guest species	454:468	a guest species in ZIF-8	454:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	1	theme	excellent	547:555	arg1	biocompatibility					557:572	excellent biocompatibility	547:572	excellent biocompatibility	547:572	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	6	2	theme	high	1051:1054	arg1	concentrations					1062:1075	the high local concentrations	1047:1075	the high local concentrations of the fluorescent probe	1047:1100	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	6	3	theme	quenching	1156:1164	arg1	rate					1166:1169	the quenching rate	1152:1169	the quenching rate	1152:1169	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	6	4	theme	quenching	1110:1118	arg1	agent					1120:1124	the quenching agent	1106:1124	the quenching agent in AuNCs/β-Gal/GOx@ZIF-8	1106:1149	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	0	5	theme	Au	72:73	arg1	nanoclusters					75:86	Au nanoclusters	72:86	Au nanoclusters	72:86	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	1	6	theme	AuNCs/β-Gal/GOx	316:330	arg1	ZIF-8					332:336	AuNCs/β-Gal/GOx@ZIF-8	316:336	AuNCs/β-Gal/GOx@ZIF-8	316:336	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	1	6	theme	AuNCs/β-Gal/GOx	316:330	arg1	oxidase					301:307	glucose oxidase	293:307	glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	293:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	2	7	theme	simple	529:534	arg1	species					462:468	a guest species	454:468	a guest species in ZIF-8	454:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	7	theme	simple	529:534	arg1	synthesis					536:544	simple synthesis	529:544	simple synthesis	529:544	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	6	8	theme	@	1144:1144	arg1	ZIF-8					1145:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	1	9	theme	@	331:331	arg1	ZIF-8					332:336	AuNCs/β-Gal/GOx@ZIF-8	316:336	AuNCs/β-Gal/GOx@ZIF-8	316:336	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	1	9	theme	@	331:331	arg1	oxidase					301:307	glucose oxidase	293:307	glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	293:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	6	10	theme	AuNCs/β-Gal/GOx	1129:1143	arg1	ZIF-8					1145:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	2	11	theme	high	578:581	arg1	species					462:468	a guest species	454:468	a guest species in ZIF-8	454:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	11	theme	high	578:581	arg1	stability					583:591	high stability	578:591	high stability	578:591	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	0	12	theme	simple	2:7	arg1	sensor					23:28	A simple and sensitive sensor	0:28	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes	0:98	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	6	13	theme	free	1201:1204	arg1	AuNCs					1206:1210	free AuNCs	1201:1210	free AuNCs	1201:1210	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	5	14	theme	Fluorescence	881:892	arg1	images					905:910	Fluorescence microscope images	881:910	Fluorescence microscope images	881:910	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	5	15	theme	microscope	894:903	arg1	images					905:910	Fluorescence microscope images	881:910	Fluorescence microscope images	881:910	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	4	16	with	structure	854:862	arg1	ZIF-8					874:878	pure ZIF-8	869:878	pure ZIF-8	869:878	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	1	17	theme	zeolite	168:174	arg1	frameworks					186:195	zeolite imidazole frameworks	168:195	zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	168:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	0	18	theme	sensitive	13:21	arg1	sensor					23:28	A simple and sensitive sensor	0:28	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes	0:98	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	0	19	theme	metal-organic	116:128	arg1	frameworks					130:139	metal-organic frameworks	116:139	metal-organic frameworks	116:139	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	5	20	from	presence	1002:1009	arg1	composite					1027:1035	the composite	1023:1035	the composite	1023:1035	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	1	21	theme	imidazole	176:184	arg1	frameworks					186:195	zeolite imidazole frameworks	168:195	zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	168:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	2	22	theme	other	386:390	arg1	nano-materials					404:417	other fluorescent nano-materials	386:417	other fluorescent nano-materials	386:417	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	3	23	theme	coating	685:691	arg1	formation					666:674	the formation	662:674	the formation of ZIF-8 coating	662:691	Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together.					
32871299	2	24	theme	small	509:513	arg1	<1 nm					521:525	<1 nm	521:525	<1 nm	521:525	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	24	theme	small	509:513	arg1	size					515:518	their extremely small size	493:518	their extremely small size (<1 nm)	493:526	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	24	theme	small	509:513	arg1	species					462:468	a guest species	454:468	a guest species in ZIF-8	454:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	6	25	from	agent	1120:1124	arg1	ZIF-8					1145:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	1	26	theme	frameworks	186:195	arg1	kind					160:163	one kind	156:163	one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	156:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	3	27	dep	promote	654:660	arg1	co-encapsulated					711:725	co-encapsulated	711:725	were co-encapsulated in ZIF-8 with the enzymes together	706:760	Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together.					
32871299	0	28	from	reactions	59:67	arg1	nanoclusters					75:86	Au nanoclusters	72:86	Au nanoclusters	72:86	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	0	28	from	reactions	59:67	arg1	enzymes					92:98	enzymes	92:98	enzymes	92:98	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	2	29	theme	distinct	431:438	arg1	species					462:468	a guest species	454:468	a guest species in ZIF-8	454:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	29	theme	distinct	431:438	arg1	advantages					440:449	distinct advantages	431:449	distinct advantages	431:449	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	4	30	dep	composite	810:818	arg1	possesses					820:828	possesses	820:828	possesses the similar crystalline structure with pure ZIF-8	820:878	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	2	31	from	species	462:468	arg1	ZIF-8					473:477	ZIF-8	473:477	ZIF-8	473:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	32	theme	fluorescent	392:402	arg1	nano-materials					404:417	other fluorescent nano-materials	386:417	other fluorescent nano-materials	386:417	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	1	33	theme	bovine	208:213	arg1	albumin					221:227	bovine serum albumin	208:227	bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	208:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	4	34	theme	similar	834:840	arg1	structure					854:862	the similar crystalline structure	830:862	the similar crystalline structure with pure ZIF-8	830:878	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	5	35	theme	AuNCs	1014:1018	arg1	presence					1002:1009	the presence	998:1009	the presence of AuNCs in the composite	998:1035	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	4	36	theme	diffraction	769:779	arg1	analysis					787:794	X-ray diffraction (XRD) analysis	763:794	X-ray diffraction (XRD) analysis	763:794	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	6	37	theme	probe	1096:1100	arg1	agent					1120:1124	the quenching agent	1106:1124	the quenching agent in AuNCs/β-Gal/GOx@ZIF-8	1106:1149	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	6	37	theme	probe	1096:1100	arg1	concentrations					1062:1075	the high local concentrations	1047:1075	the high local concentrations of the fluorescent probe	1047:1100	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	1	38	theme	glucose	293:299	arg1	ZIF-8					332:336	AuNCs/β-Gal/GOx@ZIF-8	316:336	AuNCs/β-Gal/GOx@ZIF-8	316:336	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	1	38	theme	glucose	293:299	arg1	GOx					310:312	GOx	310:312	GOx	310:312	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	1	38	theme	glucose	293:299	arg1	oxidase					301:307	glucose oxidase	293:307	glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	293:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	4	39	theme	pure	869:872	arg1	ZIF-8					874:878	pure ZIF-8	869:878	pure ZIF-8	869:878	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	4	40	theme	X-ray	763:767	arg1	XRD					782:784	XRD	782:784	XRD	782:784	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	4	40	theme	X-ray	763:767	arg1	diffraction					769:779	X-ray diffraction	763:779	X-ray diffraction (XRD) analysis	763:794	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	6	41	theme	fluorescent	1084:1094	arg1	probe					1096:1100	the fluorescent probe	1080:1100	the fluorescent probe	1080:1100	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	1	42	contain	containing	197:206	arg1	frameworks					186:195	zeolite imidazole frameworks	168:195	zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	168:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	1	42	contain	containing	197:206	arg2	albumin					221:227	bovine serum albumin	208:227	bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	208:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	5	43	dep	Fourier	913:919	arg1	transform					921:929	transform	921:929	transform infrared spectra	921:946	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	1	44	theme	serum	215:219	arg1	albumin					221:227	bovine serum albumin	208:227	bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8)	208:337	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	4	45	theme	crystalline	842:852	arg1	structure					854:862	the similar crystalline structure	830:862	the similar crystalline structure with pure ZIF-8	830:878	X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8.					
32871299	3	46	theme	bovine	611:616	arg1	albumin					624:630	the bovine serum albumin	607:630	the bovine serum albumin on their surfaces	607:648	Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together.					
32871299	5	47	theme	energy	952:957	arg1	spectroscopy					976:987	energy dispersive X-ray spectroscopy	952:987	energy dispersive X-ray spectroscopy	952:987	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	5	48	dep	transform	921:929	arg1	infrared					931:938	infrared	931:938	transform infrared spectra	921:946	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	0	49	theme	cascade	51:57	arg1	reactions					59:67	cascade reactions	51:67	cascade reactions in Au nanoclusters and enzymes	51:98	A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks.					
32871299	3	50	theme	serum	618:622	arg1	albumin					624:630	the bovine serum albumin	607:630	the bovine serum albumin on their surfaces	607:648	Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together.					
32871299	5	51	theme	dispersive	959:968	arg1	spectroscopy					976:987	energy dispersive X-ray spectroscopy	952:987	energy dispersive X-ray spectroscopy	952:987	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	5	52	attach	presence	1002:1009	arg1	composite					1027:1035	the composite	1023:1035	the composite	1023:1035	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	5	52	attach	presence	1002:1009	arg2	AuNCs					1014:1018	AuNCs	1014:1018	AuNCs	1014:1018	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	2	53	theme	guest	456:460	arg1	biocompatibility					557:572	excellent biocompatibility	547:572	excellent biocompatibility	547:572	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	53	theme	guest	456:460	arg1	species					462:468	a guest species	454:468	a guest species in ZIF-8	454:477	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	53	theme	guest	456:460	arg1	stability					583:591	high stability	578:591	high stability	578:591	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	53	theme	guest	456:460	arg1	size					515:518	their extremely small size	493:518	their extremely small size (<1 nm)	493:526	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	53	theme	guest	456:460	arg1	synthesis					536:544	simple synthesis	529:544	simple synthesis	529:544	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	2	53	theme	guest	456:460	arg1	advantages					440:449	distinct advantages	431:449	distinct advantages	431:449	Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability.					
32871299	5	54	theme	X-ray	970:974	arg1	spectroscopy					976:987	energy dispersive X-ray spectroscopy	952:987	energy dispersive X-ray spectroscopy	952:987	Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite.					
32871299	6	55	from	concentrations	1062:1075	arg1	ZIF-8					1145:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	AuNCs/β-Gal/GOx@ZIF-8	1129:1149	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	6	56	theme	local	1056:1060	arg1	concentrations					1062:1075	the high local concentrations	1047:1075	the high local concentrations of the fluorescent probe	1047:1100	Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.					
32871299	1	57	theme	Au	240:241	arg1	AuNCs					257:261	AuNCs	257:261	AuNCs	257:261	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
32871299	1	57	theme	Au	240:241	arg1	nanoclusters					243:254	Au nanoclusters	240:254	Au nanoclusters (AuNCs)	240:262	In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose.					
34073944	4	0	theme	7/3	773:775	arg1	ratio					764:768	a de-SSP/casein ratio	748:768	a de-SSP/casein ratio of 7/3	748:775	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	8	1	theme	HPLC	1293:1296	arg1	profile					1298:1304	its HPLC profile	1289:1304	its HPLC profile	1289:1304	The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra.					
34073944	9	2	theme	anti-NO	1475:1481	arg1	effects					1483:1489	novel anti-NO effects	1469:1489	novel anti-NO effects	1469:1489	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	0	3	theme	Prodigiosin	73:83	arg1	Production					49:58	the Production	45:58	the Production of Bioactive Prodigiosin	45:83	Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.					
34073944	6	4	theme	cultivation	1073:1083	arg1	time					1085:1088	a short cultivation time	1065:1088	a short cultivation time	1065:1088	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	2	5	theme	PG	325:326	arg1	preparation					310:320	the cost-effective preparation	291:320	the cost-effective preparation of PG	291:326	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	6	6	from	production	1016:1025	arg1	time					1085:1088	a short cultivation time	1065:1088	a short cultivation time	1065:1088	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	6	7	theme	PG	1013:1014	arg1	production					1016:1025	cost-effective, large-scale (5 L/pilot) PG production	973:1025	cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time	973:1088	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	6	8	theme	short	1067:1071	arg1	time					1085:1088	a short cultivation time	1065:1088	a short cultivation time	1065:1088	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	9	9	dep	effective	1398:1406	arg1	anticancer					1408:1417	anticancer	1408:1417	anticancer	1408:1417	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	2	10	theme	wastes	273:278	arg1	bioprocessing					239:251	the bioprocessing	235:251	the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG	235:326	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	7	11	theme	novel	1162:1166	arg1	combination					1095:1105	The combination	1091:1105	The combination of 0.02% K2SO4 and 0.05% K2HPO4	1091:1137	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	7	11	theme	novel	1162:1166	arg1	composition					1173:1183	a novel salt composition	1160:1183	a novel salt composition that significantly enhanced PG yield	1160:1220	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	4	12	theme	scale-up	538:545	arg1	production					547:556	PG scale-up production	535:556	PG scale-up production	535:556	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	7	13	theme	salt	1168:1171	arg1	combination					1095:1105	The combination	1091:1105	The combination of 0.02% K2SO4 and 0.05% K2HPO4	1091:1137	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	7	13	theme	salt	1168:1171	arg1	composition					1173:1183	a novel salt composition	1160:1183	a novel salt composition that significantly enhanced PG yield	1160:1220	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	7	14	theme	PG	1213:1214	arg1	yield					1216:1220	PG yield	1213:1220	PG yield	1213:1220	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	2	15	theme	chitinous	263:271	arg1	MCWs					281:284	MCWs	281:284	MCWs	281:284	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	2	15	theme	chitinous	263:271	arg1	wastes					273:278	marine chitinous wastes	256:278	marine chitinous wastes (MCWs)	256:285	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	4	16	theme	PG	535:536	arg1	production					547:556	PG scale-up production	535:556	PG scale-up production	535:556	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	4	17	theme	initial	818:824	arg1	pH					826:827	an initial pH	815:827	an initial pH of 6-7	815:834	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	2	18	theme	marine	256:261	arg1	MCWs					281:284	MCWs	281:284	MCWs	281:284	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	2	18	theme	marine	256:261	arg1	wastes					273:278	marine chitinous wastes	256:278	marine chitinous wastes (MCWs)	256:285	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	6	19	dep	cost-effective	973:986	arg1	large-scale					989:999	large-scale	989:999	large-scale	989:999	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	7	20	theme	K2SO4	1116:1120	arg1	combination					1095:1105	The combination	1091:1105	The combination of 0.02% K2SO4 and 0.05% K2HPO4	1091:1137	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	7	20	theme	K2SO4	1116:1120	arg1	composition					1173:1183	a novel salt composition	1160:1183	a novel salt composition that significantly enhanced PG yield	1160:1220	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	6	21	theme	wastes	962:967	arg1	utilization					940:950	the utilization	936:950	the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time	936:1088	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	3	22	theme	bacterial	468:476	arg1	fermentation					478:489	bacterial fermentation	468:489	bacterial fermentation using Serratia marcescens strains	468:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	23	theme	demineralized	342:354	arg1	powders					369:375	demineralized shrimp shell powders	342:375	demineralized shrimp shell powders (de-SSP)	342:384	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	23	theme	demineralized	342:354	arg1	de-SSP					378:383	de-SSP	378:383	de-SSP	378:383	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	23	theme	demineralized	342:354	arg1	source					415:420	a potential source	403:420	a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains	403:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	4	24	theme	6-7	832:834	arg1	pH					826:827	an initial pH	815:827	an initial pH of 6-7	815:834	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	6	25	theme	cost-effective	973:986	arg1	production					1016:1025	cost-effective, large-scale (5 L/pilot) PG production	973:1025	cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time	973:1088	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	1	26	theme	numerous	162:169	arg1	applications					182:193	its numerous beneficial applications	158:193	its numerous beneficial applications	158:193	Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications.					
34073944	3	27	theme	shrimp	356:361	arg1	powders					369:375	demineralized shrimp shell powders	342:375	demineralized shrimp shell powders (de-SSP)	342:384	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	27	theme	shrimp	356:361	arg1	de-SSP					378:383	de-SSP	378:383	de-SSP	378:383	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	27	theme	shrimp	356:361	arg1	source					415:420	a potential source	403:420	a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains	403:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	4	28	theme	de-SSP/casein	750:762	arg1	ratio					764:768	a de-SSP/casein ratio	748:768	a de-SSP/casein ratio of 7/3	748:775	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	1	29	theme	beneficial	171:180	arg1	applications					182:193	its numerous beneficial applications	158:193	its numerous beneficial applications	158:193	Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications.					
34073944	0	30	theme	Chitinous	24:32	arg1	Wastes					34:39	Marine Chitinous Wastes	17:39	Marine Chitinous Wastes	17:39	Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.					
34073944	4	31	theme	0.05	796:799	arg1	%					800:800	%	800:800	%	800:800	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	3	32	theme	Serratia	497:504	arg1	strains					517:523	Serratia marcescens strains	497:523	Serratia marcescens strains	497:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	33	theme	potential	405:413	arg1	powders					369:375	demineralized shrimp shell powders	342:375	demineralized shrimp shell powders (de-SSP)	342:384	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	33	theme	potential	405:413	arg1	source					415:420	a potential source	403:420	a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains	403:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	0	34	theme	Marine	17:22	arg1	Wastes					34:39	Marine Chitinous Wastes	17:39	Marine Chitinous Wastes	17:39	Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.					
34073944	9	35	dep	tested	1358:1363	arg1	moderated					1431:1439	moderated	1431:1439	moderated antioxidant activities, and novel anti-NO effects	1431:1489	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	4	36	theme	novel-designed	686:699	arg1	broth					709:713	a novel-designed culture broth	684:713	a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7	684:834	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	6	37	theme	shrimp	955:960	arg1	wastes					962:967	shrimp wastes	955:967	shrimp wastes	955:967	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	3	38	dep	Serratia	497:504	arg1	marcescens					506:515	marcescens	506:515	marcescens	506:515	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	9	39	theme	antioxidant	1441:1451	arg1	activities					1453:1462	antioxidant activities	1441:1462	antioxidant activities	1441:1462	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	4	40	dep	%	733:733	arg1	sources					739:745	C/N sources	735:745	1.60% C/N sources (a de-SSP/casein ratio of 7/3)	729:776	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	9	41	theme	purified	1337:1344	arg1	PG					1346:1347	The purified PG	1333:1347	The purified PG	1333:1347	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	7	42	theme	%	1114:1114	arg1	K2SO4					1116:1120	0.02% K2SO4	1110:1120	0.02% K2SO4	1110:1120	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	4	43	theme	bioreactor	585:594	arg1	system					596:601	a 15 L bioreactor system	578:601	a 15 L bioreactor system	578:601	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	0	44	theme	Wastes	34:39	arg1	Bioprocessing					0:12	Bioprocessing	0:12	Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.	0:84	Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.					
34073944	4	45	theme	%	800:800	arg1	K2HPO4					802:807	0.05% K2HPO4	796:807	0.05% K2HPO4	796:807	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	8	46	theme	red	1227:1229	arg1	PG					1270:1271	PG	1270:1271	PG	1270:1271	The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra.					
34073944	8	46	theme	red	1227:1229	arg1	compound					1231:1238	The red compound	1223:1238	The red compound	1223:1238	The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra.					
34073944	2	47	theme	study	212:216	arg1	aim					200:202	The aim	196:202	The aim of this study	196:216	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	4	48	theme	L	583:583	arg1	system					596:601	a 15 L bioreactor system	578:601	a 15 L bioreactor system	578:601	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	3	49	theme	shell	363:367	arg1	powders					369:375	demineralized shrimp shell powders	342:375	demineralized shrimp shell powders (de-SSP)	342:384	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	49	theme	shell	363:367	arg1	de-SSP					378:383	de-SSP	378:383	de-SSP	378:383	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	49	theme	shell	363:367	arg1	source					415:420	a potential source	403:420	a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains	403:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	8	50	theme	UV/vis	1317:1322	arg1	spectra					1324:1330	UV/vis spectra	1317:1330	UV/vis spectra	1317:1330	The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra.					
34073944	4	51	theme	%	783:783	arg1	K2SO4					785:789	0.02% K2SO4	779:789	0.02% K2SO4	779:789	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	6	52	theme	high	1032:1035	arg1	productivity					1037:1048	high productivity	1032:1048	high productivity (6200 mg/L)	1032:1060	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	6	52	theme	high	1032:1035	arg1	mg/L					1056:1059	6200 mg/L	1051:1059	6200 mg/L	1051:1059	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	9	53	theme	novel	1469:1473	arg1	effects					1483:1489	novel anti-NO effects	1469:1489	novel anti-NO effects	1469:1489	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	4	54	theme	0.02	779:782	arg1	%					783:783	%	783:783	%	783:783	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	4	55	theme	C/N	735:737	arg1	sources					739:745	C/N sources	735:745	1.60% C/N sources (a de-SSP/casein ratio of 7/3)	729:776	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	9	56	theme	effective	1398:1406	arg1	activities					1419:1428	effective anticancer activities	1398:1428	effective anticancer activities	1398:1428	The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.					
34073944	7	57	theme	K2HPO4	1132:1137	arg1	combination					1095:1105	The combination	1091:1105	The combination of 0.02% K2SO4 and 0.05% K2HPO4	1091:1137	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	7	57	theme	K2HPO4	1132:1137	arg1	composition					1173:1183	a novel salt composition	1160:1183	a novel salt composition that significantly enhanced PG yield	1160:1220	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	4	58	theme	broth	709:713	arg1	L					679:679	5 L	677:679	5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7	677:834	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	4	59	theme	highest	612:618	arg1	yield					620:624	the highest yield	608:624	the highest yield (6200 mg/L)	608:636	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	4	59	theme	highest	612:618	arg1	mg/L					632:635	6200 mg/L	627:635	6200 mg/L	627:635	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	2	60	theme	cost-effective	295:308	arg1	preparation					310:320	the cost-effective preparation	291:320	the cost-effective preparation of PG	291:326	The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG.					
34073944	1	61	theme	microbial	96:104	arg1	PG					119:120	PG	119:120	PG	119:120	Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications.					
34073944	1	61	theme	microbial	96:104	arg1	prodigiosin					106:116	microbial prodigiosin	96:116	microbial prodigiosin (PG)	96:121	Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications.					
34073944	6	62	dep	large-scale	989:999	arg1	L/pilot					1004:1010	5 L/pilot	1002:1010	5 L/pilot	1002:1010	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	4	63	theme	culture	701:707	arg1	broth					709:713	a novel-designed culture broth	684:713	a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7	684:834	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	3	64	theme	carbon/nitrogen	425:439	arg1	powders					369:375	demineralized shrimp shell powders	342:375	demineralized shrimp shell powders (de-SSP)	342:384	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	3	64	theme	carbon/nitrogen	425:439	arg1	source					415:420	a potential source	403:420	a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains	403:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	7	65	theme	0.02	1110:1113	arg1	%					1114:1114	%	1114:1114	%	1114:1114	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	0	66	theme	Bioactive	63:71	arg1	Prodigiosin					73:83	Bioactive Prodigiosin	63:83	Bioactive Prodigiosin	63:83	Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.					
34073944	7	67	theme	0.05	1126:1129	arg1	%					1130:1130	%	1130:1130	%	1130:1130	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	4	68	dep	sources	739:745	arg1	ratio					764:768	a de-SSP/casein ratio	748:768	a de-SSP/casein ratio of 7/3	748:775	Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6-7.					
34073944	3	69	theme	PG	451:452	arg1	production					454:463	PG production	451:463	PG production by bacterial fermentation using Serratia marcescens strains	451:523	Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains.					
34073944	6	70	with	production	1016:1025	arg1	productivity					1037:1048	high productivity	1032:1048	high productivity (6200 mg/L)	1032:1060	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	6	70	with	production	1016:1025	arg1	mg/L					1056:1059	6200 mg/L	1051:1059	6200 mg/L	1051:1059	This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time.					
34073944	7	71	theme	%	1130:1130	arg1	K2HPO4					1132:1137	0.05% K2HPO4	1126:1137	0.05% K2HPO4	1126:1137	The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield.					
34073944	1	72	theme	much	136:139	arg1	attention					141:149	much attention	136:149	much attention	136:149	Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications.					
34052276	4	0	theme	linear	773:778	arg1	manners					780:786	approximately linear manners	759:786	approximately linear manners	759:786	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
34052276	7	1	theme	promising	1221:1229	arg1	potential					1231:1239	promising potential	1221:1239	promising potential	1221:1239	The obtained results suggest that these composite gels have promising potential in bone repair and regeneration.					
34052276	6	2	theme	osteogenic	1140:1149	arg1	capacity					1151:1158	certain osteogenic capacity	1132:1158	certain osteogenic capacity	1132:1158	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	1	3	theme	porous	225:230	arg1	architecture					232:243	radially porous architecture	216:243	radially porous architecture	216:243	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	6	4	from	detection	992:1000	arg1	constructs					1081:1090	the cell-gel constructs	1068:1090	the cell-gel constructs	1068:1090	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	2	5	from	pH	533:534	arg1	thermo-responsive					482:498	thermo-responsive	482:498	thermo-responsive	482:498	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	2	5	from	pH	533:534	arg1	gels					460:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	0	6	theme	tissue	138:143	arg1	engineering					145:155	bone tissue engineering	133:155	bone tissue engineering	133:155	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	6	7	theme	cell-gel	1072:1079	arg1	constructs					1081:1090	the cell-gel constructs	1068:1090	the cell-gel constructs	1068:1090	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	0	8	theme	bone	133:136	arg1	engineering					145:155	bone tissue engineering	133:155	bone tissue engineering	133:155	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	6	9	contain	had	1128:1130	arg2	capacity					1151:1158	certain osteogenic capacity	1132:1158	certain osteogenic capacity	1132:1158	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	6	9	contain	had	1128:1130	arg1	gels					1123:1126	these composite gels	1107:1126	these composite gels	1107:1126	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	6	10	theme	phosphatase	1014:1024	arg1	activity					1026:1033	alkaline phosphatase activity	1005:1033	alkaline phosphatase activity	1005:1033	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	4	11	theme	few	794:796	arg1	weeks					798:802	a few weeks	792:802	a few weeks	792:802	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
34052276	5	12	theme	cells	918:922	arg1	growth					892:897	the growth	888:897	the growth of seeded MC3T3-E1 cells	888:922	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	5	12	theme	cells	918:922	arg1	deposition					933:942	the deposition	929:942	the deposition of matrix components	929:963	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	4	13	from	range	750:754	arg1	manners					780:786	approximately linear manners	759:786	approximately linear manners	759:786	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
34052276	4	14	theme	dose	745:748	arg1	range					750:754	an effective dose range	732:754	an effective dose range in approximately linear manners	732:786	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
34052276	5	15	theme	cell-gel	820:827	arg1	constructs					829:838	the cell-gel constructs	816:838	the cell-gel constructs	816:838	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	1	16	with	nanoparticles	197:209	arg1	architecture					232:243	radially porous architecture	216:243	radially porous architecture	216:243	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	3	17	theme	elastic	625:631	arg1	characteristics					633:647	mechanically strong and elastic characteristics	601:647	mechanically strong and elastic characteristics	601:647	They were also detected to have mechanically strong and elastic characteristics.					
34052276	4	18	theme	effective	735:743	arg1	range					750:754	an effective dose range	732:754	an effective dose range in approximately linear manners	732:786	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
34052276	2	19	with	thermo-responsive	482:498	arg1	injectability					554:566	well-defined injectability	541:566	well-defined injectability	541:566	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	0	20	theme	Thermo-responsive	0:16	arg1	chitosan/silk					18:30	Thermo-responsive chitosan/silk	0:30	Thermo-responsive chitosan/silk	0:30	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	2	21	theme	composite	450:458	arg1	thermo-responsive					482:498	thermo-responsive	482:498	thermo-responsive	482:498	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	2	21	theme	composite	450:458	arg1	gels					460:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	0	22	theme	mesoporous	61:70	arg1	hydrogels					79:87	mesoporous silica hydrogels	61:87	mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering	61:155	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	6	23	theme	mineralization	1050:1063	arg1	detection					992:1000	the detection	988:1000	the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs	988:1090	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	7	24	theme	obtained	1165:1172	arg1	results					1174:1180	The obtained results	1161:1180	The obtained results	1161:1180	The obtained results suggest that these composite gels have promising potential in bone repair and regeneration.					
34052276	5	25	theme	MC3T3-E1	909:916	arg1	cells					918:922	seeded MC3T3-E1 cells	902:922	seeded MC3T3-E1 cells	902:922	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	2	26	theme	physiological	503:515	arg1	temperature					517:527	physiological temperature	503:527	physiological temperature	503:527	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	6	27	theme	matrix	1043:1048	arg1	mineralization					1050:1063	the matrix mineralization	1039:1063	the matrix mineralization	1039:1063	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	5	28	theme	components	954:963	arg1	growth					892:897	the growth	888:897	the growth of seeded MC3T3-E1 cells	888:922	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	5	28	theme	components	954:963	arg1	deposition					933:942	the deposition	929:942	the deposition of matrix components	929:963	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	3	29	theme	mechanically	601:612	arg1	characteristics					633:647	mechanically strong and elastic characteristics	601:647	mechanically strong and elastic characteristics	601:647	They were also detected to have mechanically strong and elastic characteristics.					
34052276	5	30	theme	composite	858:866	arg1	gels					868:871	the composite gels	854:871	the composite gels	854:871	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	1	31	used	used	287:290	arg2	they					277:280	they	277:280	they	277:280	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	5	32	theme	matrix	947:952	arg1	components					954:963	matrix components	947:963	matrix components	947:963	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	6	33	theme	certain	1132:1138	arg1	capacity					1151:1158	certain osteogenic capacity	1132:1158	certain osteogenic capacity	1132:1158	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	0	34	theme	silica	72:77	arg1	hydrogels					79:87	mesoporous silica hydrogels	61:87	mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering	61:155	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	1	35	theme	crosslinked	364:374	arg1	hydrogel					386:393	covalently crosslinked composite hydrogel	353:393	covalently crosslinked composite hydrogel using genipin as a crosslinker	353:424	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	5	36	from	Studies	805:811	arg1	constructs					829:838	the cell-gel constructs	816:838	the cell-gel constructs	816:838	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	4	37	theme	Si	714:715	arg1	ions					717:720	bioactive Si ions	704:720	bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks	704:802	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
34052276	0	38	theme	strong	94:99	arg1	characteristics					113:127	strong and elastic characteristics	94:127	strong and elastic characteristics	94:127	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	1	39	theme	composite	376:384	arg1	hydrogel					386:393	covalently crosslinked composite hydrogel	353:393	covalently crosslinked composite hydrogel using genipin as a crosslinker	353:424	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	1	40	theme	Amino-functionalized	158:177	arg1	nanoparticles					197:209	Amino-functionalized mesoporous silica nanoparticles	158:209	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture	158:243	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	7	41	theme	bone	1244:1247	arg1	repair					1249:1254	bone repair	1244:1254	bone repair	1244:1254	The obtained results suggest that these composite gels have promising potential in bone repair and regeneration.					
34052276	1	42	theme	hydrogel	386:393	arg1	type					345:348	a type	343:348	a type of covalently crosslinked composite hydrogel using genipin as a crosslinker	343:424	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	3	43	contain	have	596:599	arg1	They					569:572	They	569:572	They	569:572	They were also detected to have mechanically strong and elastic characteristics.					
34052276	3	43	contain	have	596:599	arg2	characteristics					633:647	mechanically strong and elastic characteristics	601:647	mechanically strong and elastic characteristics	601:647	They were also detected to have mechanically strong and elastic characteristics.					
34052276	7	44	theme	composite	1201:1209	arg1	gels					1211:1214	these composite gels	1195:1214	these composite gels	1195:1214	The obtained results suggest that these composite gels have promising potential in bone repair and regeneration.					
34052276	1	45	link	crosslinked	364:374	arg1	hydrogel					386:393	covalently crosslinked composite hydrogel	353:393	covalently crosslinked composite hydrogel using genipin as a crosslinker	353:424	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	1	46	theme	mesoporous	179:188	arg1	nanoparticles					197:209	Amino-functionalized mesoporous silica nanoparticles	158:209	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture	158:243	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	6	47	theme	alkaline	1005:1012	arg1	phosphatase					1014:1024	alkaline phosphatase	1005:1024	alkaline phosphatase activity	1005:1033	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	1	48	theme	silica	190:195	arg1	nanoparticles					197:209	Amino-functionalized mesoporous silica nanoparticles	158:209	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture	158:243	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	6	49	theme	composite	1113:1121	arg1	gels					1123:1126	these composite gels	1107:1126	these composite gels	1107:1126	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	0	50	theme	elastic	105:111	arg1	characteristics					113:127	strong and elastic characteristics	94:127	strong and elastic characteristics	94:127	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	7	51	contain	have	1216:1219	arg2	potential					1231:1239	promising potential	1221:1239	promising potential	1221:1239	The obtained results suggest that these composite gels have promising potential in bone repair and regeneration.					
34052276	7	51	contain	have	1216:1219	arg1	gels					1211:1214	these composite gels	1195:1214	these composite gels	1195:1214	The obtained results suggest that these composite gels have promising potential in bone repair and regeneration.					
34052276	2	52	theme	achieved	441:448	arg1	thermo-responsive					482:498	thermo-responsive	482:498	thermo-responsive	482:498	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	2	52	theme	achieved	441:448	arg1	gels					460:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	1	53	theme	silk	306:309	arg1	fibroin					311:317	silk fibroin	306:317	silk fibroin	306:317	Amino-functionalized mesoporous silica nanoparticles with radially porous architecture were optimally synthesized, and they were used together with silk fibroin and chitosan to produce a type of covalently crosslinked composite hydrogel using genipin as a crosslinker.					
34052276	2	54	theme	well-defined	541:552	arg1	injectability					554:566	well-defined injectability	541:566	well-defined injectability	541:566	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	2	55	from	temperature	517:527	arg1	thermo-responsive					482:498	thermo-responsive	482:498	thermo-responsive	482:498	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	2	55	from	temperature	517:527	arg1	gels					460:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels	427:463	The optimally achieved composite gels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability.					
34052276	5	56	theme	seeded	902:907	arg1	cells					918:922	seeded MC3T3-E1 cells	902:922	seeded MC3T3-E1 cells	902:922	Studies on the cell-gel constructs revealed that the composite gels well supported the growth of seeded MC3T3-E1 cells, and the deposition of matrix components.					
34052276	0	57	with	hydrogels	79:87	arg1	characteristics					113:127	strong and elastic characteristics	94:127	strong and elastic characteristics	94:127	Thermo-responsive chitosan/silk fibroin/amino-functionalized mesoporous silica hydrogels with strong and elastic characteristics for bone tissue engineering.					
34052276	3	58	theme	strong	614:619	arg1	characteristics					633:647	mechanically strong and elastic characteristics	601:647	mechanically strong and elastic characteristics	601:647	They were also detected to have mechanically strong and elastic characteristics.					
34052276	6	59	theme	activity	1026:1033	arg1	detection					992:1000	the detection	988:1000	the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs	988:1090	Results obtained from the detection of alkaline phosphatase activity and the matrix mineralization in the cell-gel constructs confirmed that these composite gels had certain osteogenic capacity.					
34052276	4	60	theme	bioactive	704:712	arg1	ions					717:720	bioactive Si ions	704:720	bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks	704:802	In addition, these gels showed the ability to release bioactive Si ions suited to an effective dose range in approximately linear manners for a few weeks.					
32138617	8	0	theme	decellularization	1022:1038	arg1	treatment					1060:1068	The combined decellularization protocol comprising treatment	1009:1068	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100	1009:1103	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	4	1	theme	Triton	523:528	arg1	X-100					530:534	Triton X-100	523:534	Triton X-100 (TX100)	523:542	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	1	theme	Triton	523:528	arg1	TX100					537:541	TX100	537:541	TX100	537:541	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	5	2	theme	decellularization	676:692	arg1	protocols					694:702	five combined decellularization protocols	662:702	five combined decellularization protocols based on the results of phase I	662:734	In decellularization phase II, we systematically compared five combined decellularization protocols based on the results of phase I to identify the optimal method.					
32138617	5	3	theme	phase	728:732	arg1	results					717:723	the results	713:723	the results of phase I	713:734	In decellularization phase II, we systematically compared five combined decellularization protocols based on the results of phase I to identify the optimal method.					
32138617	0	4	theme	mechanical	77:86	arg1	properties					103:112	mechanical and structural properties	77:112	mechanical and structural properties	77:112	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	9	5	theme	heparinized	1236:1246	arg1	scaffolds					1263:1271	the heparinized decellularized scaffolds	1232:1271	the heparinized decellularized scaffolds	1232:1271	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	8	6	with	treatment	1060:1068	arg1	SDS					1080:1082	0.5% SDS	1075:1082	0.5% SDS	1075:1082	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	4	7	theme	sodium	494:499	arg1	SDS					517:519	SDS	517:519	SDS	517:519	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	7	theme	sodium	494:499	arg1	dodecylsulfate					501:514	sodium dodecylsulfate	494:514	sodium dodecylsulfate (SDS)	494:520	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	7	8	theme	optimal	907:913	arg1	scaffolds					930:938	optimal decellularized scaffolds	907:938	optimal decellularized scaffolds	907:938	We further immobilized heparin to optimal decellularized scaffolds and determined its anticoagulant activity and mechanical properties.					
32138617	3	9	theme	matrix	402:407	arg1	composition					415:425	the extracellular matrix (ECM) composition	384:425	the extracellular matrix (ECM) composition	384:425	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	0	10	from	Characterization	0:15	arg1	properties					103:112	mechanical and structural properties	77:112	mechanical and structural properties	77:112	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	8	11	theme	comprising	1049:1058	arg1	treatment					1060:1068	The combined decellularization protocol comprising treatment	1009:1068	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100	1009:1103	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	7	12	theme	decellularized	915:928	arg1	scaffolds					930:938	optimal decellularized scaffolds	907:938	optimal decellularized scaffolds	907:938	We further immobilized heparin to optimal decellularized scaffolds and determined its anticoagulant activity and mechanical properties.					
32138617	0	13	theme	structural	92:101	arg1	properties					103:112	mechanical and structural properties	77:112	mechanical and structural properties	77:112	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	10	14	theme	promising	1442:1450	arg1	direction					1452:1460	a promising direction	1440:1460	a promising direction for small-diameter vascular grafts	1440:1495	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	8	15	theme	0.5	1075:1077	arg1	%					1078:1078	%	1078:1078	%	1078:1078	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	9	16	contain	had	1282:1284	arg2	activity					1310:1317	sustained anticoagulant activity	1286:1317	sustained anticoagulant activity	1286:1317	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	9	16	contain	had	1282:1284	arg1	scaffolds					1263:1271	the heparinized decellularized scaffolds	1232:1271	the heparinized decellularized scaffolds	1232:1271	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	8	17	theme	%	1078:1078	arg1	SDS					1080:1082	0.5% SDS	1075:1082	0.5% SDS	1075:1082	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	6	18	theme	mechanical	823:832	arg1	properties					834:843	mechanical properties	823:843	mechanical properties	823:843	These protocols tested cell removal, ECM preservation, mechanical properties, and residual cytotoxicity.					
32138617	3	19	theme	extracellular	388:400	arg1	ECM					410:412	ECM	410:412	ECM	410:412	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	3	19	theme	extracellular	388:400	arg1	matrix					402:407	extracellular matrix	388:407	the extracellular matrix (ECM) composition	384:425	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	3	20	theme	decellularization	276:292	arg1	phases					294:299	two decellularization phases	272:299	two decellularization phases	272:299	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	1	21	theme	small-diameter	191:204	arg1	vessels					212:218	small-diameter blood vessels	191:218	small-diameter blood vessels	191:218	Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels.					
32138617	9	22	theme	native	1362:1367	arg1	vessels					1369:1375	native vessels	1362:1375	native vessels	1362:1375	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	8	23	theme	cellular	1133:1140	arg1	contents					1142:1149	the cellular contents	1129:1149	the cellular contents	1129:1149	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	1	24	from	approach	148:155	arg1	engineering					167:177	tissue engineering	160:177	tissue engineering	160:177	Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels.					
32138617	1	25	theme	blood	206:210	arg1	vessels					212:218	small-diameter blood vessels	191:218	small-diameter blood vessels	191:218	Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels.					
32138617	8	26	theme	combined	1013:1020	arg1	treatment					1060:1068	The combined decellularization protocol comprising treatment	1009:1068	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100	1009:1103	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	5	27	theme	combined	667:674	arg1	protocols					694:702	five combined decellularization protocols	662:702	five combined decellularization protocols based on the results of phase I	662:734	In decellularization phase II, we systematically compared five combined decellularization protocols based on the results of phase I to identify the optimal method.					
32138617	6	28	theme	ECM	805:807	arg1	preservation					809:820	ECM preservation	805:820	ECM preservation	805:820	These protocols tested cell removal, ECM preservation, mechanical properties, and residual cytotoxicity.					
32138617	10	29	dep	represent	1430:1438	arg1	although					1498:1505	although	1498:1505	although	1498:1505	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	0	30	theme	heparinized	22:32	arg1	scaffold					49:56	a heparinized decellularized scaffold	20:56	a heparinized decellularized scaffold	20:56	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	4	31	theme	different	559:567	arg1	concentrations					569:582	different concentrations	559:582	different concentrations	559:582	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	5	32	theme	decellularization	607:623	arg1	phase					625:629	decellularization phase II	607:632	decellularization phase II	607:632	In decellularization phase II, we systematically compared five combined decellularization protocols based on the results of phase I to identify the optimal method.					
32138617	9	33	theme	decellularized	1248:1261	arg1	scaffolds					1263:1271	the heparinized decellularized scaffolds	1232:1271	the heparinized decellularized scaffolds	1232:1271	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	9	34	theme	anticoagulant	1296:1308	arg1	activity					1310:1317	sustained anticoagulant activity	1286:1317	sustained anticoagulant activity	1286:1317	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	4	35	theme	exposure	588:595	arg1	times					597:601	exposure times	588:601	exposure times	588:601	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	9	36	theme	similar	1329:1335	arg1	properties					1348:1357	similar mechanical properties	1329:1357	similar mechanical properties	1329:1357	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	10	37	theme	heparinized	1393:1403	arg1	scaffolds					1420:1428	heparinized decellularized scaffolds	1393:1428	heparinized decellularized scaffolds	1393:1428	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	0	38	theme	scaffold	49:56	arg1	Characterization					0:15	Characterization	0:15	Characterization of a heparinized decellularized scaffold	0:56	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	0	38	theme	scaffold	49:56	arg1	effects					66:72	its effects	62:72	its effects on mechanical and structural properties	62:112	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	10	39	theme	in vivo	1515:1521	arg1	studies					1523:1529	further in vivo studies	1507:1529	further in vivo studies	1507:1529	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	0	40	theme	decellularized	34:47	arg1	scaffold					49:56	a heparinized decellularized scaffold	20:56	a heparinized decellularized scaffold	20:56	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	4	41	from	concentrations	569:582	arg1	trypsin					548:554	trypsin	548:554	trypsin at different concentrations and exposure times	548:601	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	41	from	concentrations	569:582	arg1	X-100					530:534	Triton X-100	523:534	Triton X-100 (TX100)	523:542	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	41	from	concentrations	569:582	arg1	TX100					537:541	TX100	537:541	TX100	537:541	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	41	from	concentrations	569:582	arg1	SDS					517:519	SDS	517:519	SDS	517:519	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	41	from	concentrations	569:582	arg1	dodecylsulfate					501:514	sodium dodecylsulfate	494:514	sodium dodecylsulfate (SDS)	494:520	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	42	from	times	597:601	arg1	trypsin					548:554	trypsin	548:554	trypsin at different concentrations and exposure times	548:601	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	42	from	times	597:601	arg1	X-100					530:534	Triton X-100	523:534	Triton X-100 (TX100)	523:542	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	42	from	times	597:601	arg1	TX100					537:541	TX100	537:541	TX100	537:541	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	42	from	times	597:601	arg1	SDS					517:519	SDS	517:519	SDS	517:519	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	4	42	from	times	597:601	arg1	dodecylsulfate					501:514	sodium dodecylsulfate	494:514	sodium dodecylsulfate (SDS)	494:520	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	3	43	theme	mechanical	431:440	arg1	properties					442:451	mechanical properties	431:451	mechanical properties	431:451	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	5	44	theme	optimal	752:758	arg1	method					760:765	the optimal method	748:765	the optimal method	748:765	In decellularization phase II, we systematically compared five combined decellularization protocols based on the results of phase I to identify the optimal method.					
32138617	0	45	from	effects	66:72	arg1	properties					103:112	mechanical and structural properties	77:112	mechanical and structural properties	77:112	Characterization of a heparinized decellularized scaffold and its effects on mechanical and structural properties.					
32138617	6	46	theme	cell	791:794	arg1	removal					796:802	cell removal	791:802	cell removal	791:802	These protocols tested cell removal, ECM preservation, mechanical properties, and residual cytotoxicity.					
32138617	8	47	theme	mechanical	1168:1177	arg1	properties					1179:1188	the mechanical properties	1164:1188	the mechanical properties	1164:1188	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	9	48	theme	mechanical	1337:1346	arg1	properties					1348:1357	similar mechanical properties	1329:1357	similar mechanical properties	1329:1357	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	7	49	theme	anticoagulant	959:971	arg1	activity					973:980	its anticoagulant activity	955:980	its anticoagulant activity	955:980	We further immobilized heparin to optimal decellularized scaffolds and determined its anticoagulant activity and mechanical properties.					
32138617	7	50	theme	mechanical	986:995	arg1	properties					997:1006	mechanical properties	986:1006	mechanical properties	986:1006	We further immobilized heparin to optimal decellularized scaffolds and determined its anticoagulant activity and mechanical properties.					
32138617	3	51	theme	optimal	315:321	arg1	scaffold					338:345	an optimal decellularized scaffold	312:345	an optimal decellularized scaffold	312:345	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	4	52	theme	decellularization	457:473	arg1	phase					475:479	decellularization phase I	457:481	decellularization phase I	457:481	In decellularization phase I, we tested sodium dodecylsulfate (SDS), Triton X-100 (TX100) and trypsin at different concentrations and exposure times.					
32138617	10	53	theme	further	1507:1513	arg1	studies					1523:1529	further in vivo studies	1507:1529	further in vivo studies	1507:1529	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	1	54	theme	promising	138:146	arg1	approach					148:155	a promising approach	136:155	a promising approach in tissue engineering to generate small-diameter blood vessels	136:218	Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels.					
32138617	1	54	theme	promising	138:146	arg1	Decellularization					115:131	Decellularization	115:131	Decellularization	115:131	Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels.					
32138617	10	55	theme	small-diameter	1466:1479	arg1	grafts					1490:1495	small-diameter vascular grafts	1466:1495	small-diameter vascular grafts	1466:1495	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	3	56	theme	decellularized	323:336	arg1	scaffold					338:345	an optimal decellularized scaffold	312:345	an optimal decellularized scaffold	312:345	We performed two decellularization phases to develop an optimal decellularized scaffold and analyze the relationship between the extracellular matrix (ECM) composition and mechanical properties.					
32138617	8	57	theme	ECM	1194:1196	arg1	architecture					1198:1209	ECM architecture	1194:1209	ECM architecture	1194:1209	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	10	58	theme	decellularized	1405:1418	arg1	scaffolds					1420:1428	heparinized decellularized scaffolds	1393:1428	heparinized decellularized scaffolds	1393:1428	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	6	59	theme	residual	850:857	arg1	cytotoxicity					859:870	residual cytotoxicity	850:870	residual cytotoxicity	850:870	These protocols tested cell removal, ECM preservation, mechanical properties, and residual cytotoxicity.					
32138617	10	60	theme	vascular	1481:1488	arg1	grafts					1490:1495	small-diameter vascular grafts	1466:1495	small-diameter vascular grafts	1466:1495	In conclusion, heparinized decellularized scaffolds represent a promising direction for small-diameter vascular grafts, although further in vivo studies are needed.					
32138617	9	61	theme	sustained	1286:1294	arg1	activity					1310:1317	sustained anticoagulant activity	1286:1317	sustained anticoagulant activity	1286:1317	In addition, the heparinized decellularized scaffolds not only had sustained anticoagulant activity, but also similar mechanical properties to native vessels.					
32138617	8	62	theme	protocol	1040:1047	arg1	treatment					1060:1068	The combined decellularization protocol comprising treatment	1009:1068	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100	1009:1103	The combined decellularization protocol comprising treatment with 0.5% SDS followed by 1% TX100 could completely remove the cellular contents and preserve the mechanical properties and ECM architecture better.					
32138617	1	63	theme	tissue	160:165	arg1	engineering					167:177	tissue engineering	160:177	tissue engineering	160:177	Decellularization is a promising approach in tissue engineering to generate small-diameter blood vessels.					
32718655	2	0	dep	nanoparticles	308:320	arg1	fibers					343:348	-coated pulp fibers	330:348	-coated pulp fibers	330:348	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	7	1	theme	intrinsic	945:953	arg1	capability					969:978	the intrinsic antibacterial capability	941:978	the intrinsic antibacterial capability of Ag NPs	941:988	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	4	2	theme	separation	538:547	arg1	efficiency					549:558	This outstanding separation efficiency	521:558	This outstanding separation efficiency	521:558	This outstanding separation efficiency was maintained over 20 separation cycles.					
32718655	8	3	theme	composite	1090:1098	arg1	paper					1100:1104	the composite paper	1086:1104	the composite paper that was mainly derived from sustainable pulp fibers	1086:1157	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	6	4	theme	bacteria-contaminated	861:881	arg1	emulsions					883:891	bacteria-contaminated emulsions	861:891	bacteria-contaminated emulsions due to a physical barrier effect	861:924	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	2	5	theme	cellulose	354:362	arg1	nanofibrils					364:374	cellulose nanofibrils	354:374	cellulose nanofibrils (CNFs)	354:381	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	2	5	theme	cellulose	354:362	arg1	CNFs					377:380	CNFs	377:380	CNFs	377:380	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	4	6	theme	outstanding	526:536	arg1	efficiency					549:558	This outstanding separation efficiency	521:558	This outstanding separation efficiency	521:558	This outstanding separation efficiency was maintained over 20 separation cycles.					
32718655	5	7	theme	satisfactory	634:645	arg1	performance					658:668	satisfactory separation performance	634:668	satisfactory separation performance for water-in-oil emulsions	634:695	The composite paper also showed satisfactory separation performance for water-in-oil emulsions after modification to render the surface hydrophobic.					
32718655	5	8	theme	separation	647:656	arg1	performance					658:668	satisfactory separation performance	634:668	satisfactory separation performance for water-in-oil emulsions	634:695	The composite paper also showed satisfactory separation performance for water-in-oil emulsions after modification to render the surface hydrophobic.					
32718655	2	9	theme	Ag	305:306	arg1	nanoparticles					308:320	Ag nanoparticles	305:320	Ag nanoparticles (Ag NPs)	305:329	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	2	9	theme	Ag	305:306	arg1	NPs					326:328	Ag NPs	323:328	Ag NPs	323:328	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	6	10	theme	composite	773:781	arg1	pore					795:798	the composite a nanoscale pore	769:798	the composite a nanoscale pore	769:798	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	2	11	theme	-coated	330:336	arg1	fibers					343:348	-coated pulp fibers	330:348	-coated pulp fibers	330:348	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	5	12	theme	water-in-oil	674:685	arg1	emulsions					687:695	water-in-oil emulsions	674:695	water-in-oil emulsions	674:695	The composite paper also showed satisfactory separation performance for water-in-oil emulsions after modification to render the surface hydrophobic.					
32718655	1	13	theme	Bio-based	98:106	arg1	membranes					108:116	Bio-based membranes	98:116	Bio-based membranes for emulsion separation	98:140	Bio-based membranes for emulsion separation are sustainable, low-cost, renewable, biodegradable, and nontoxic.					
32718655	8	14	theme	sustainable	1135:1145	arg1	fibers					1152:1157	sustainable pulp fibers	1135:1157	sustainable pulp fibers	1135:1157	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	5	15	theme	composite	606:614	arg1	paper					616:620	The composite paper	602:620	The composite paper	602:620	The composite paper also showed satisfactory separation performance for water-in-oil emulsions after modification to render the surface hydrophobic.					
32718655	3	16	theme	obtained	388:395	arg1	superhydrophilic					429:444	superhydrophilic	429:444	superhydrophilic	429:444	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	3	16	theme	obtained	388:395	arg1	paper					419:423	The obtained Ag-Pulp/CNF composite paper	384:423	The obtained Ag-Pulp/CNF composite paper	384:423	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	4	17	theme	separation	583:592	arg1	cycles					594:599	20 separation cycles	580:599	20 separation cycles	580:599	This outstanding separation efficiency was maintained over 20 separation cycles.					
32718655	7	18	theme	NPs	986:988	arg1	capability					969:978	the intrinsic antibacterial capability	941:978	the intrinsic antibacterial capability of Ag NPs	941:988	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	0	19	theme	sandwich-like	30:42	arg1	paper					66:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	Sustainable and antibacterial sandwich-like Ag-Pulp/CNF composite paper for oil/water separation.					
32718655	2	20	theme	Ag	323:324	arg1	nanoparticles					308:320	Ag nanoparticles	305:320	Ag nanoparticles (Ag NPs)	305:329	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	2	20	theme	Ag	323:324	arg1	NPs					326:328	Ag NPs	323:328	Ag NPs	323:328	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	7	21	theme	strong	1021:1026	arg1	performance					1042:1052	strong antibacterial performance	1021:1052	strong antibacterial performance	1021:1052	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	3	22	theme	separation	463:472	arg1	efficiency					474:483	a 96 % separation efficiency	456:483	a 96 % separation efficiency for various oil-in-water emulsions	456:518	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	0	23	theme	antibacterial	16:28	arg1	paper					66:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	Sustainable and antibacterial sandwich-like Ag-Pulp/CNF composite paper for oil/water separation.					
32718655	8	24	theme	pulp	1147:1150	arg1	fibers					1152:1157	sustainable pulp fibers	1135:1157	sustainable pulp fibers	1135:1157	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	8	25	attach	derived	1122:1128	arg1	fibers					1152:1157	sustainable pulp fibers	1135:1157	sustainable pulp fibers	1135:1157	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	8	25	attach	derived	1122:1128	arg2	paper					1100:1104	the composite paper	1086:1104	the composite paper that was mainly derived from sustainable pulp fibers	1086:1157	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	0	26	theme	composite	56:64	arg1	paper					66:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	Sustainable and antibacterial sandwich-like Ag-Pulp/CNF composite paper for oil/water separation.					
32718655	8	27	theme	potential	1169:1177	arg1	performance					1179:1189	potential performance	1169:1189	potential performance for oil/water separation and water purification	1169:1237	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	3	28	theme	96	458:459	arg1	%					461:461	%	461:461	%	461:461	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	0	29	theme	Ag-Pulp/CNF	44:54	arg1	paper					66:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	antibacterial sandwich-like Ag-Pulp/CNF composite paper	16:70	Sustainable and antibacterial sandwich-like Ag-Pulp/CNF composite paper for oil/water separation.					
32718655	6	30	theme	barrier	911:917	arg1	effect					919:924	a physical barrier effect	900:924	a physical barrier effect	900:924	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	3	31	theme	%	461:461	arg1	efficiency					474:483	a 96 % separation efficiency	456:483	a 96 % separation efficiency for various oil-in-water emulsions	456:518	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	3	32	theme	Ag-Pulp/CNF	397:407	arg1	superhydrophilic					429:444	superhydrophilic	429:444	superhydrophilic	429:444	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	3	32	theme	Ag-Pulp/CNF	397:407	arg1	paper					419:423	The obtained Ag-Pulp/CNF composite paper	384:423	The obtained Ag-Pulp/CNF composite paper	384:423	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	6	33	theme	physical	902:909	arg1	barrier					911:917	a physical barrier	900:917	a physical barrier effect	900:924	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	2	34	theme	composite	248:256	arg1	paper					258:262	an antibacterial sandwich-like composite paper	217:262	an antibacterial sandwich-like composite paper	217:262	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	3	35	theme	oil-in-water	497:508	arg1	emulsions					510:518	various oil-in-water emulsions	489:518	various oil-in-water emulsions	489:518	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	3	36	theme	composite	409:417	arg1	superhydrophilic					429:444	superhydrophilic	429:444	superhydrophilic	429:444	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	3	36	theme	composite	409:417	arg1	paper					419:423	The obtained Ag-Pulp/CNF composite paper	384:423	The obtained Ag-Pulp/CNF composite paper	384:423	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	7	37	theme	composite	995:1003	arg1	paper					1005:1009	the composite paper	991:1009	the composite paper	991:1009	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	5	38	theme	hydrophobic	738:748	arg1	surface					730:736	the surface	726:736	the surface hydrophobic	726:748	The composite paper also showed satisfactory separation performance for water-in-oil emulsions after modification to render the surface hydrophobic.					
32718655	2	39	theme	antibacterial	220:232	arg1	paper					258:262	an antibacterial sandwich-like composite paper	217:262	an antibacterial sandwich-like composite paper	217:262	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	2	40	theme	sandwich-like	234:246	arg1	paper					258:262	an antibacterial sandwich-like composite paper	217:262	an antibacterial sandwich-like composite paper	217:262	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	2	41	theme	facile	284:289	arg1	process					291:297	a facile process	282:297	a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs)	282:381	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	7	42	theme	antibacterial	1028:1040	arg1	performance					1042:1052	strong antibacterial performance	1021:1052	strong antibacterial performance	1021:1052	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	2	43	theme	pulp	338:341	arg1	fibers					343:348	-coated pulp fibers	330:348	-coated pulp fibers	330:348	Herein, an antibacterial sandwich-like composite paper was fabricated by a facile process using Ag nanoparticles (Ag NPs)-coated pulp fibers and cellulose nanofibrils (CNFs).					
32718655	1	44	theme	emulsion	122:129	arg1	separation					131:140	emulsion separation	122:140	emulsion separation	122:140	Bio-based membranes for emulsion separation are sustainable, low-cost, renewable, biodegradable, and nontoxic.					
32718655	8	45	theme	oil/water	1195:1203	arg1	separation					1205:1214	oil/water separation	1195:1214	oil/water separation	1195:1214	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	6	46	theme	due	893:895	arg1	emulsions					883:891	bacteria-contaminated emulsions	861:891	bacteria-contaminated emulsions due to a physical barrier effect	861:924	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	3	47	theme	various	489:495	arg1	emulsions					510:518	various oil-in-water emulsions	489:518	various oil-in-water emulsions	489:518	The obtained Ag-Pulp/CNF composite paper was superhydrophilic, allowing a 96 % separation efficiency for various oil-in-water emulsions.					
32718655	7	48	theme	antibacterial	955:967	arg1	capability					969:978	the intrinsic antibacterial capability	941:978	the intrinsic antibacterial capability of Ag NPs	941:988	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	8	49	theme	water	1220:1224	arg1	purification					1226:1237	water purification	1220:1237	water purification	1220:1237	Thus, the composite paper that was mainly derived from sustainable pulp fibers exhibited potential performance for oil/water separation and water purification.					
32718655	7	50	theme	Ag	983:984	arg1	NPs					986:988	Ag NPs	983:988	Ag NPs	983:988	Attributed to the intrinsic antibacterial capability of Ag NPs, the composite paper exhibited strong antibacterial performance against Escherichia coli.					
32718655	6	51	theme	bacteria	832:839	arg1	bacteria					832:839	bacteria	832:839	bacteria	832:839	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	6	51	theme	bacteria	832:839	arg1	%					827:827	nearly 100 %	816:827	nearly 100 % of bacteria	816:839	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32718655	0	52	theme	oil/water	76:84	arg1	separation					86:95	oil/water separation	76:95	oil/water separation	76:95	Sustainable and antibacterial sandwich-like Ag-Pulp/CNF composite paper for oil/water separation.					
32718655	6	53	theme	nanoscale	785:793	arg1	pore					795:798	the composite a nanoscale pore	769:798	the composite a nanoscale pore	769:798	The CNFs provided the composite a nanoscale pore, which rejected nearly 100 % of bacteria when used to filter bacteria-contaminated emulsions due to a physical barrier effect.					
32901954	6	0	theme	polymerized	1659:1669	arg1	protein					1676:1682	polymerized whey protein	1659:1682	polymerized whey protein	1659:1682	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	6	0	theme	polymerized	1659:1669	arg1	agent					1707:1711	a gelation agent	1696:1711	a gelation agent	1696:1711	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	3	1	from	composition	692:702	arg1	pH					716:717	pH	716:717	pH	716:717	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	2	theme	compact	1401:1407	arg1	structure					1424:1432	a compact and denser gel structure	1399:1432	a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1399:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	6	3	theme	almond	1736:1741	arg1	alternatives					1750:1761	symbiotic almond yogurt alternatives	1726:1761	symbiotic almond yogurt alternatives	1726:1761	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	1	4	theme	yogurt-like	191:201	arg1	product					203:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	3	5	from	viscosity	720:728	arg1	pH					716:717	pH	716:717	pH	716:717	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	5	6	theme	yogurt	1601:1606	arg1	alternative					1608:1618	symbiotic almond yogurt alternative	1584:1618	symbiotic almond yogurt alternative	1584:1618	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	3	7	theme	probiotic	735:743	arg1	survivability					745:757	probiotic survivability	735:757	probiotic survivability	735:757	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	8	theme	%	1447:1447	arg1	PWP					1449:1451	0.6% PWP	1444:1451	0.6% PWP	1444:1451	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	1	9	dep	YF-L02	268:273	arg1	thermophilus					290:301	Streptococcus thermophilus	276:301	a plant-based starter YF-L02 (Streptococcus thermophilus	246:301	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	4	10	theme	samples	1018:1024	arg1	differences					851:861	the differences	847:861	the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples	847:1024	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	3	11	from	changes	705:711	arg1	pH					716:717	pH	716:717	pH	716:717	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	0	12	theme	gelation	150:157	arg1	agent					159:163	a gelation agent	148:163	a gelation agent	148:163	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	0	12	theme	gelation	150:157	arg1	protein					137:143	polymerized whey protein	120:143	polymerized whey protein	120:143	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	3	13	theme	with/without	608:619	arg1	groups					601:606	Two groups	597:606	Two groups with/without calcium citrate and vitamin D2	597:650	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	3	13	theme	with/without	608:619	arg1	citrate					629:635	with/without calcium citrate	608:635	with/without calcium citrate	608:635	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	14	dep	decreased	1026:1034	arg1	1					821:821	1	821:821	1	821:821	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	2	15	theme	alternative	584:594	arg1	formula					555:561	the formula	551:561	the formula of the almond yogurt alternative	551:594	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	4	16	theme	first	1275:1279	arg1	group					1313:1317	the control group	1301:1317	the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1301:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	16	theme	first	1275:1279	arg1	weeks					1283:1287	the first 4 weeks	1271:1287	the first 4 weeks	1271:1287	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	17	theme	xanthan	1496:1502	arg1	gum					1504:1506	0.05% xanthan gum	1490:1506	0.05% xanthan gum	1490:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	2	18	theme	almond	570:575	arg1	alternative					584:594	the almond yogurt alternative	566:594	the almond yogurt alternative	566:594	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	6	19	theme	texture	1788:1794	arg1	and probiotic survivability					1796:1822	comparable physical texture and probiotic survivability	1768:1822	comparable physical texture and probiotic survivability to dairy yogurt during storage	1768:1853	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	4	20	theme	0.3	1474:1476	arg1	%					1477:1477	%	1477:1477	%	1477:1477	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	2	21	theme	%	518:518	arg1	gum					528:530	0.05% xanthan gum	514:530	0.05% xanthan gum	514:530	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	6	22	theme	comparable	1768:1777	arg1	and probiotic survivability					1796:1822	comparable physical texture and probiotic survivability	1768:1822	comparable physical texture and probiotic survivability to dairy yogurt during storage	1768:1853	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	0	23	theme	symbiotic	78:86	arg1	almond					88:93	symbiotic almond	78:93	symbiotic almond	78:93	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	4	24	theme	pectin	1479:1484	arg1	presence					1462:1469	the presence	1458:1469	the presence of 0.3% pectin and 0.05% xanthan gum	1458:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	25	theme	fortified	941:949	arg1	samples					951:957	the fortified samples	937:957	the fortified samples	937:957	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	25	theme	fortified	941:949	arg1	significant					968:978	significant	968:978	significant	968:978	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	25	theme	fortified	941:949	arg1	>					983:983	P > 0.05	981:988	P > 0.05	981:988	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	25	theme	fortified	941:949	arg1	2					993:993	2	993:993	2	993:993	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	7	26	theme	foods	1929:1933	arg1	development					1892:1902	the development	1888:1902	the development of plant-based fermented foods	1888:1933	This technology may be used for the development of plant-based fermented foods.					
32901954	4	27	theme	control	1305:1311	arg1	group					1313:1317	the control group	1301:1317	the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1301:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	27	theme	control	1305:1311	arg1	weeks					1283:1287	the first 4 weeks	1271:1287	the first 4 weeks	1271:1287	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	1	28	theme	symbiotic	174:182	arg1	product					203:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	3	29	theme	vitamin	641:647	arg1	groups					601:606	Two groups	597:606	Two groups with/without calcium citrate and vitamin D2	597:650	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	3	29	theme	vitamin	641:647	arg1	D2					649:650	vitamin D2	641:650	vitamin D2	641:650	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	3	30	from	°C	779:780	arg1	storage					766:772	storage	766:772	storage at 4 °C for 10 weeks	766:793	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	31	theme	electron	1368:1375	arg1	microscopy					1377:1386	scanning electron microscopy	1359:1386	scanning electron microscopy	1359:1386	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	0	32	theme	whey	132:135	arg1	agent					159:163	a gelation agent	148:163	a gelation agent	148:163	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	0	32	theme	whey	132:135	arg1	protein					137:143	polymerized whey protein	120:143	polymerized whey protein	120:143	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	0	33	dep	using	114:118	arg1	alternative					102:112	alternative	102:112	alternative	102:112	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	4	34	theme	P	981:981	arg1	samples					951:957	the fortified samples	937:957	the fortified samples	937:957	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	34	theme	P	981:981	arg1	pH					870:871	the pH	866:871	the pH	866:871	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	34	theme	P	981:981	arg1	>					983:983	P > 0.05	981:988	P > 0.05	981:988	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	34	theme	P	981:981	arg1	significant					968:978	significant	968:978	significant	968:978	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	34	theme	P	981:981	arg1	2					993:993	2	993:993	2	993:993	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	0	35	theme	Physicochemical	0:14	arg1	properties					36:45	Physicochemical and microstructural properties	0:45	Physicochemical and microstructural properties	0:45	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	1	36	theme	Lactobacillus	304:316	arg1	subsp					330:334	Lactobacillus delbrueckii subsp	304:334	Lactobacillus delbrueckii subsp	304:334	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	1	36	theme	Lactobacillus	304:316	arg1	YF-L02					268:273	a plant-based starter YF-L02	246:273	a plant-based starter YF-L02 (Streptococcus thermophilus	246:301	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	6	37	used	used	1688:1691	arg2	protein					1676:1682	polymerized whey protein	1659:1682	polymerized whey protein	1659:1682	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	6	37	used	used	1688:1691	arg2	agent					1707:1711	a gelation agent	1696:1711	a gelation agent	1696:1711	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	2	38	theme	whey	477:480	arg1	PWP					491:493	PWP	491:493	PWP	491:493	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	2	38	theme	whey	477:480	arg1	protein					482:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein	337:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP)	337:494	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	0	39	theme	microstructural	20:34	arg1	properties					36:45	Physicochemical and microstructural properties	0:45	Physicochemical and microstructural properties	0:45	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	6	40	theme	PRACTICAL	1621:1629	arg1	APPLICATION					1631:1641	PRACTICAL APPLICATION	1621:1641	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.	1621:1854	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	2	41	theme	%	463:463	arg1	PWP					491:493	PWP	491:493	PWP	491:493	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	2	41	theme	%	463:463	arg1	protein					482:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein	337:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP)	337:494	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	2	42	theme	0.3	497:499	arg1	%					500:500	%	500:500	%	500:500	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	4	43	theme	gel	1420:1422	arg1	structure					1424:1432	a compact and denser gel structure	1399:1432	a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1399:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	6	44	dep	APPLICATION	1631:1641	arg1	used					1688:1691	used	1688:1691	was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage	1684:1853	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	4	45	theme	animalis	1146:1153	arg1	populations					1111:1121	populations	1111:1121	the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1107:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	5	46	theme	proper	1539:1544	arg1	agent					1555:1559	a proper gelation agent	1537:1559	a proper gelation agent for the formulation of symbiotic almond yogurt alternative	1537:1618	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	5	46	theme	proper	1539:1544	arg1	PWP					1524:1526	PWP	1524:1526	PWP	1524:1526	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	1	47	theme	starter	260:266	arg1	subsp					330:334	Lactobacillus delbrueckii subsp	304:334	Lactobacillus delbrueckii subsp	304:334	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	1	47	theme	starter	260:266	arg1	YF-L02					268:273	a plant-based starter YF-L02	246:273	a plant-based starter YF-L02 (Streptococcus thermophilus	246:301	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	7	48	theme	fermented	1919:1927	arg1	foods					1929:1933	plant-based fermented foods	1907:1933	plant-based fermented foods	1907:1933	This technology may be used for the development of plant-based fermented foods.					
32901954	6	49	theme	whey	1671:1674	arg1	protein					1676:1682	polymerized whey protein	1659:1682	polymerized whey protein	1659:1682	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	6	49	theme	whey	1671:1674	arg1	agent					1707:1711	a gelation agent	1696:1711	a gelation agent	1696:1711	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	4	50	dep	significant	968:978	arg1	pH					870:871	the pH	866:871	the pH	866:871	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	50	dep	significant	968:978	arg1	samples					951:957	the fortified samples	937:957	the fortified samples	937:957	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	50	dep	significant	968:978	arg1	2					993:993	2	993:993	2	993:993	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	50	dep	significant	968:978	arg1	>					983:983	P > 0.05	981:988	P > 0.05	981:988	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	50	dep	significant	968:978	arg1	significant					968:978	significant	968:978	significant	968:978	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	51	theme	weeks	832:836	arg1	storage					838:844	10 weeks storage	829:844	10 weeks storage	829:844	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	5	52	from	agent	1555:1559	arg1	conclusion					1512:1521	conclusion	1512:1521	conclusion	1512:1521	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	4	53	theme	acidophilus	1229:1239	arg1	population					1212:1221	the population	1208:1221	the population of L. acidophilus	1208:1239	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	54	theme	0.6	1444:1446	arg1	%					1447:1447	%	1447:1447	%	1447:1447	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	7	55	used	used	1879:1882	arg2	technology					1861:1870	This technology	1856:1870	This technology	1856:1870	This technology may be used for the development of plant-based fermented foods.					
32901954	4	56	dep	increased	1077:1085	arg1	populations					1111:1121	populations	1111:1121	the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1107:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	56	dep	increased	1077:1085	arg1	3					1104:1104	3	1104:1104	3	1104:1104	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	1	57	theme	almond	184:189	arg1	product					203:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	6	58	theme	symbiotic	1726:1734	arg1	alternatives					1750:1761	symbiotic almond yogurt alternatives	1726:1761	symbiotic almond yogurt alternatives	1726:1761	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	5	59	theme	almond	1594:1599	arg1	alternative					1608:1618	symbiotic almond yogurt alternative	1584:1618	symbiotic almond yogurt alternative	1584:1618	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	3	60	dep	groups	601:606	arg1	groups					601:606	Two groups	597:606	Two groups with/without calcium citrate and vitamin D2	597:650	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	3	60	dep	groups	601:606	arg1	citrate					629:635	with/without calcium citrate	608:635	with/without calcium citrate	608:635	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	3	60	dep	groups	601:606	arg1	D2					649:650	vitamin D2	641:650	vitamin D2	641:650	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	61	theme	yogurt	1011:1016	arg1	samples					1018:1024	both yogurt samples	1006:1024	both yogurt samples	1006:1024	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	5	62	theme	alternative	1608:1618	arg1	formulation					1569:1579	the formulation	1565:1579	the formulation of symbiotic almond yogurt alternative	1565:1618	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	6	63	theme	gelation	1698:1705	arg1	protein					1676:1682	polymerized whey protein	1659:1682	polymerized whey protein	1659:1682	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	6	63	theme	gelation	1698:1705	arg1	agent					1707:1711	a gelation agent	1696:1711	a gelation agent	1696:1711	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	4	64	theme	%	1494:1494	arg1	gum					1504:1506	0.05% xanthan gum	1490:1506	0.05% xanthan gum	1490:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	65	dep	examined	1347:1354	arg1	4					1321:1321	4	1321:1321	4	1321:1321	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	66	theme	gum	1504:1506	arg1	presence					1462:1469	the presence	1458:1469	the presence of 0.3% pectin and 0.05% xanthan gum	1458:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	significant					968:978	significant	968:978	significant	968:978	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	2					993:993	2	993:993	2	993:993	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	pH					870:871	the pH	866:871	the pH	866:871	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	>					983:983	P > 0.05	981:988	P > 0.05	981:988	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	the					996:998	the	996:998	the	996:998	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	samples					951:957	the fortified samples	937:957	the fortified samples	937:957	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	67	dep	samples	1018:1024	arg1	pH					1000:1001	pH	1000:1001	pH	1000:1001	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	2	68	theme	yogurt	577:582	arg1	alternative					584:594	the almond yogurt alternative	566:594	the almond yogurt alternative	566:594	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	4	69	theme	%	1477:1477	arg1	pectin					1479:1484	0.3% pectin	1474:1484	0.3% pectin	1474:1484	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	6	70	theme	physical	1779:1786	arg1	and probiotic survivability					1796:1822	comparable physical texture and probiotic survivability	1768:1822	comparable physical texture and probiotic survivability to dairy yogurt during storage	1768:1853	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	0	71	theme	almond	88:93	arg1	properties					36:45	Physicochemical and microstructural properties	0:45	Physicochemical and microstructural properties	0:45	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	0	71	theme	almond	88:93	arg1	survivability					61:73	probiotic survivability	51:73	probiotic survivability of symbiotic almond	51:93	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	3	72	theme	calcium	621:627	arg1	groups					601:606	Two groups	597:606	Two groups with/without calcium citrate and vitamin D2	597:650	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	3	72	theme	calcium	621:627	arg1	citrate					629:635	with/without calcium citrate	608:635	with/without calcium citrate	608:635	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	2	73	theme	0.05	514:517	arg1	%					518:518	%	518:518	%	518:518	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	1	74	theme	plain	168:172	arg1	product					203:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product	166:209	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	4	75	theme	paracasei	1129:1137	arg1	populations					1111:1121	populations	1111:1121	the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1107:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	6	76	theme	yogurt	1743:1748	arg1	alternatives					1750:1761	symbiotic almond yogurt alternatives	1726:1761	symbiotic almond yogurt alternatives	1726:1761	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	0	77	theme	polymerized	120:130	arg1	agent					159:163	a gelation agent	148:163	a gelation agent	148:163	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	0	77	theme	polymerized	120:130	arg1	protein					137:143	polymerized whey protein	120:143	polymerized whey protein	120:143	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	2	78	theme	bulgaricus	337:346	arg1	PWP					491:493	PWP	491:493	PWP	491:493	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	2	78	theme	bulgaricus	337:346	arg1	protein					482:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein	337:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP)	337:494	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	4	79	theme	probiotic	889:897	arg1	survivability					899:911	probiotic survivability	889:911	probiotic survivability	889:911	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	2	80	theme	xanthan	520:526	arg1	gum					528:530	0.05% xanthan gum	514:530	0.05% xanthan gum	514:530	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	3	81	from	survivability	745:757	arg1	pH					716:717	pH	716:717	pH	716:717	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	82	theme	scanning	1359:1366	arg1	microscopy					1377:1386	scanning electron microscopy	1359:1386	scanning electron microscopy	1359:1386	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	1	83	theme	delbrueckii	318:328	arg1	subsp					330:334	Lactobacillus delbrueckii subsp	304:334	Lactobacillus delbrueckii subsp	304:334	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	1	83	theme	delbrueckii	318:328	arg1	YF-L02					268:273	a plant-based starter YF-L02	246:273	a plant-based starter YF-L02 (Streptococcus thermophilus	246:301	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	6	84	theme	dairy	1827:1831	arg1	yogurt					1833:1838	dairy yogurt	1827:1838	dairy yogurt	1827:1838	PRACTICAL APPLICATION: In this study, polymerized whey protein was used as a gelation agent to formulate symbiotic almond yogurt alternatives with comparable physical texture and probiotic survivability to dairy yogurt during storage.					
32901954	2	85	theme	polymerized	465:475	arg1	PWP					491:493	PWP	491:493	PWP	491:493	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	2	85	theme	polymerized	465:475	arg1	protein					482:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein	337:488	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP)	337:494	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	0	86	theme	probiotic	51:59	arg1	survivability					61:73	probiotic survivability	51:73	probiotic survivability of symbiotic almond	51:93	Physicochemical and microstructural properties and probiotic survivability of symbiotic almond yogurt alternative using polymerized whey protein as a gelation agent.					
32901954	4	87	dep	group	1313:1317	arg1	examined					1347:1354	examined	1347:1354	examined	1347:1354	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	2	88	theme	%	500:500	arg1	pectin					502:507	0.3% pectin	497:507	0.3% pectin	497:507	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	3	89	theme	chemical	683:690	arg1	composition					692:702	chemical composition	683:702	chemical composition	683:702	Two groups with/without calcium citrate and vitamin D2 were prepared and analyzed for chemical composition, changes in pH, viscosity, and probiotic survivability during storage at 4 °C for 10 weeks.					
32901954	4	90	theme	denser	1413:1418	arg1	structure					1424:1432	a compact and denser gel structure	1399:1432	a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum	1399:1506	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	4	91	theme	0.05	1490:1493	arg1	%					1494:1494	%	1494:1494	%	1494:1494	The results showed that (1) over 10 weeks storage, the differences in the pH, viscosity, and probiotic survivability between the control and the fortified samples were not significant (P > 0.05); (2) the pH of both yogurt samples decreased 0.2 units while their viscosity slightly increased during storage; (3) the populations of L. paracasei and B. animalis remained above 106 cfu/g during the storage, whereas the population of L. acidophilus decreased dramatically during the first 4 weeks, especially the control group; (4) the microstructure was examined by scanning electron microscopy, revealing a compact and denser gel structure formed by 0.6% PWP with the presence of 0.3% pectin and 0.05% xanthan gum.					
32901954	5	92	theme	symbiotic	1584:1592	arg1	alternative					1608:1618	symbiotic almond yogurt alternative	1584:1618	symbiotic almond yogurt alternative	1584:1618	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	1	93	theme	plant-based	248:258	arg1	subsp					330:334	Lactobacillus delbrueckii subsp	304:334	Lactobacillus delbrueckii subsp	304:334	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	1	93	theme	plant-based	248:258	arg1	YF-L02					268:273	a plant-based starter YF-L02	246:273	a plant-based starter YF-L02 (Streptococcus thermophilus	246:301	A plain symbiotic almond yogurt-like product was formulated and developed using a plant-based starter YF-L02 (Streptococcus thermophilus, Lactobacillus delbrueckii subsp.					
32901954	7	94	theme	plant-based	1907:1917	arg1	foods					1929:1933	plant-based fermented foods	1907:1933	plant-based fermented foods	1907:1933	This technology may be used for the development of plant-based fermented foods.					
32901954	2	95	theme	inulin	452:457	arg1	%					463:463	inulin; 0.6%	452:463	inulin; 0.6%	452:463	bulgaricus supplemented with Lactobacillus acidophilus, Lactobacillus paracasei, and Bifidobacterium animalis) and inulin; 0.6% polymerized whey protein (PWP), 0.3% pectin, and 0.05% xanthan gum were optimized for the formula of the almond yogurt alternative.					
32901954	5	96	theme	gelation	1546:1553	arg1	agent					1555:1559	a proper gelation agent	1537:1559	a proper gelation agent for the formulation of symbiotic almond yogurt alternative	1537:1618	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
32901954	5	96	theme	gelation	1546:1553	arg1	PWP					1524:1526	PWP	1524:1526	PWP	1524:1526	In conclusion, PWP might be a proper gelation agent for the formulation of symbiotic almond yogurt alternative.					
33421637	2	0	theme	pooled	574:579	arg1	sera					587:590	pooled human sera	574:590	the pooled human sera fractionation by SMME	570:612	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	1	1	dep	polymer	307:313	arg1	such					316:319	such	316:319	such	316:319	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	3	2	theme	fixation	648:655	arg1	method					657:662	a fixation method	646:662	a fixation method for the lectin blotting of SMME-separated glycoproteins	646:718	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	6	3	theme	simple	1256:1261	arg1	fractionation					1275:1287	simple, rapid sera fractionation	1256:1287	simple, rapid sera fractionation	1256:1287	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	5	4	from	γ-bands	1112:1118	arg1	glycans					1085:1091	N-linked and O-linked glycans	1063:1091	N-linked and O-linked glycans from the separated γ-bands	1063:1118	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	1	5	theme	separated	422:430	arg1	bands					432:436	the separated bands	418:436	the separated bands	418:436	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	5	6	theme	α2,8-sialylated	1174:1188	arg1	O-glycans					1190:1198	α2,8-sialylated O-glycans	1174:1198	α2,8-sialylated O-glycans contained in mouse sera glycoproteins	1174:1236	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	3	7	theme	lectin	672:677	arg1	blotting					679:686	the lectin blotting	668:686	the lectin blotting of SMME-separated glycoproteins	668:718	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	8	8	theme	fixation	1683:1690	arg1	method					1692:1697	a fixation method	1681:1697	a fixation method	1681:1697	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	4	9	theme	protein	823:829	arg1	samples					831:837	protein samples	823:837	protein samples	823:837	It showed that the amount of protein samples required for SMME were reduced more than 4-fold than that of the process of SDS-PAGE.					
33421637	5	10	from	patterns	988:995	arg1	mice					1040:1043	Fut8+/+ and Fut8-/- mice	1020:1043	Fut8+/+ and Fut8-/- mice	1020:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	1	11	theme	matrix	198:203	arg1	SMME					222:225	SMME	222:225	SMME	222:225	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	1	11	theme	matrix	198:203	arg1	electrophoresis					205:219	molecular matrix electrophoresis	188:219	molecular matrix electrophoresis (SMME)	188:226	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	7	12	theme	mouse	1611:1615	arg1	serum					1617:1621	mouse serum	1611:1621	mouse serum separated by SMME	1611:1639	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	2	13	theme	sera	512:515	arg1	fractionation					517:529	mouse sera fractionation	506:529	mouse sera fractionation	506:529	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	4	14	theme	samples	831:837	arg1	amount					813:818	the amount	809:818	the amount of protein samples required for SMME	809:855	It showed that the amount of protein samples required for SMME were reduced more than 4-fold than that of the process of SDS-PAGE.					
33421637	4	14	theme	samples	831:837	arg1	samples					831:837	protein samples	823:837	protein samples	823:837	It showed that the amount of protein samples required for SMME were reduced more than 4-fold than that of the process of SDS-PAGE.					
33421637	3	15	theme	glycoproteins	706:718	arg1	blotting					679:686	the lectin blotting	668:686	the lectin blotting of SMME-separated glycoproteins	668:718	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	7	16	theme	fraction	1599:1606	arg1	components					1580:1589	the major protein components	1562:1589	the major protein components of each fraction in mouse serum separated by SMME	1562:1639	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	6	17	theme	glycan	1290:1295	arg1	differences					1307:1317	glycan profiling differences	1290:1317	glycan profiling differences between the bands of two samples	1290:1350	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	8	18	theme	SMME-separated	1722:1735	arg1	glycoproteins					1737:1749	SMME-separated glycoproteins	1722:1749	SMME-separated glycoproteins	1722:1749	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	3	19	theme	SMME	737:740	arg1	membranes					742:750	the SMME membranes	733:750	the SMME membranes	733:750	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	8	20	theme	glycan	1904:1909	arg1	differences					1921:1931	glycan profiling differences	1904:1931	glycan profiling differences between two samples in both research and clinical settings	1904:1990	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	5	21	theme	serum	1000:1004	arg1	proteins					1006:1013	serum proteins	1000:1013	serum proteins from Fut8+/+ and Fut8-/- mice	1000:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	7	22	theme	protein	1572:1578	arg1	components					1580:1589	the major protein components	1562:1589	the major protein components of each fraction in mouse serum separated by SMME	1562:1639	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	1	23	theme	glycan	399:404	arg1	analysis					406:413	glycan analysis	399:413	glycan analysis of the separated bands	399:436	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	1	24	theme	novel	142:146	arg1	technique					159:167	a novel separation technique	140:167	a novel separation technique	140:167	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	5	25	theme	O-linked	1076:1083	arg1	glycans					1085:1091	N-linked and O-linked glycans	1063:1091	N-linked and O-linked glycans from the separated γ-bands	1063:1118	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	8	26	gly	glycoproteins	1737:1749	arg1	glycoproteins					1737:1749	SMME-separated glycoproteins	1722:1749	SMME-separated glycoproteins	1722:1749	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	0	27	theme	molecular	81:89	arg1	electrophoresis					98:112	supported molecular matrix electrophoresis	71:112	supported molecular matrix electrophoresis	71:112	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	8	28	theme	glycosylation	1783:1795	arg1	patterns					1797:1804	glycosylation patterns	1783:1804	glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice	1783:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	8	29	theme	research	1961:1968	arg1	settings					1983:1990	both research and clinical settings	1956:1990	settings	1983:1990	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	5	30	link	N-linked	1063:1070	arg1	glycans					1085:1091	N-linked and O-linked glycans	1063:1091	N-linked and O-linked glycans from the separated γ-bands	1063:1118	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	4	31	theme	SDS-PAGE	915:922	arg1	process					904:910	the process	900:910	the process of SDS-PAGE	900:922	It showed that the amount of protein samples required for SMME were reduced more than 4-fold than that of the process of SDS-PAGE.					
33421637	8	32	theme	clinical	1974:1981	arg1	settings					1983:1990	both research and clinical settings	1956:1990	settings	1983:1990	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	5	33	theme	N-linked	1063:1070	arg1	glycans					1085:1091	N-linked and O-linked glycans	1063:1091	N-linked and O-linked glycans from the separated γ-bands	1063:1118	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	0	34	theme	serum	38:42	arg1	glycoproteins					44:56	mouse serum glycoproteins	32:56	mouse serum glycoproteins	32:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	8	35	theme	glycoproteins	1815:1827	arg1	patterns					1797:1804	glycosylation patterns	1783:1804	glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice	1783:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	1	36	with	compatible	383:392	arg1	analysis					406:413	glycan analysis	399:413	glycan analysis of the separated bands	399:436	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	5	37	gly	glycosylation	974:986	arg1	proteins					1006:1013	serum proteins	1000:1013	serum proteins from Fut8+/+ and Fut8-/- mice	1000:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	3	38	gly	glycoproteins	706:718	arg1	glycoproteins					706:718	SMME-separated glycoproteins	691:718	SMME-separated glycoproteins	691:718	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	0	39	theme	supported	71:79	arg1	electrophoresis					98:112	supported molecular matrix electrophoresis	71:112	supported molecular matrix electrophoresis	71:112	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	0	40	theme	Rapid	0:4	arg1	analysis					20:27	Rapid glycosylation analysis	0:27	Rapid glycosylation analysis of mouse serum glycoproteins	0:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	8	41	theme	Fut8+/+	1834:1840	arg1	mice					1854:1857	Fut8+/+ and Fut8-/- mice	1834:1857	Fut8+/+ and Fut8-/- mice	1834:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	8	42	from	mice	1854:1857	arg1	patterns					1797:1804	glycosylation patterns	1783:1804	glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice	1783:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	8	42	from	mice	1854:1857	arg1	glycoproteins					1815:1827	serum glycoproteins	1809:1827	serum glycoproteins from Fut8+/+ and Fut8-/- mice	1809:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	5	43	link	O-linked	1076:1083	arg1	glycans					1085:1091	N-linked and O-linked glycans	1063:1091	N-linked and O-linked glycans from the separated γ-bands	1063:1118	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	0	44	theme	mouse	32:36	arg1	glycoproteins					44:56	mouse serum glycoproteins	32:56	mouse serum glycoproteins	32:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	1	45	theme	hydrophilic	295:305	arg1	polymer					307:313	a hydrophilic polymer	293:313	a hydrophilic polymer (such as polyvinyl alcohol)	293:341	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	7	46	theme	SMME	1495:1498	arg1	application					1480:1490	the first application	1470:1490	the first application of SMME	1470:1498	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	0	47	gly	glycosylation	6:18	arg1	glycoproteins					44:56	mouse serum glycoproteins	32:56	mouse serum glycoproteins	32:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	5	48	theme	separated	1102:1110	arg1	γ-bands					1112:1118	the separated γ-bands	1098:1118	the separated γ-bands	1098:1118	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	0	49	theme	glycoproteins	44:56	arg1	analysis					20:27	Rapid glycosylation analysis	0:27	Rapid glycosylation analysis of mouse serum glycoproteins	0:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	2	50	from	fractionation	592:604	arg1	differences					553:563	their differences	547:563	their differences from the pooled human sera fractionation by SMME	547:612	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	5	51	theme	glycosylation	974:986	arg1	patterns					988:995	glycosylation patterns	974:995	glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice	974:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	6	52	dep	responsible	1405:1415	arg1	mechanism					1390:1398	the underlying mechanism	1375:1398	the underlying mechanism that responsible for related diseases	1375:1436	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	1	53	contain	has	350:352	arg1	it					347:348	it	347:348	it	347:348	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	1	53	contain	has	350:352	arg2	compatible					383:392	compatible	383:392	compatible	383:392	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	1	53	contain	has	350:352	arg2	characteristics					358:372	the characteristics	354:372	the characteristics that are compatible with glycan analysis of the separated bands	354:436	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	8	54	theme	Fut8-/-	1846:1852	arg1	mice					1854:1857	Fut8+/+ and Fut8-/- mice	1834:1857	Fut8+/+ and Fut8-/- mice	1834:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	2	55	theme	sera	587:590	arg1	fractionation					592:604	the pooled human sera fractionation	570:604	the pooled human sera fractionation by SMME	570:612	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	8	56	from	differences	1921:1931	arg1	settings					1983:1990	both research and clinical settings	1956:1990	settings	1983:1990	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	6	57	theme	related	1421:1427	arg1	diseases					1429:1436	related diseases	1421:1436	related diseases	1421:1436	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	1	58	theme	PVDF	257:260	arg1	membrane					262:269	a PVDF membrane	255:269	a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol)	255:341	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	3	59	theme	SMME-separated	691:704	arg1	glycoproteins					706:718	SMME-separated glycoproteins	691:718	SMME-separated glycoproteins	691:718	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	1	60	theme	bands	432:436	arg1	analysis					406:413	glycan analysis	399:413	glycan analysis of the separated bands	399:436	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	6	61	dep	simple	1256:1261	arg1	rapid					1264:1268	rapid	1264:1268	rapid	1264:1268	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	6	62	theme	samples	1344:1350	arg1	bands					1331:1335	the bands	1327:1335	the bands of two samples	1327:1350	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	1	63	theme	molecular	188:196	arg1	SMME					222:225	SMME	222:225	SMME	222:225	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	1	63	theme	molecular	188:196	arg1	electrophoresis					205:219	molecular matrix electrophoresis	188:219	molecular matrix electrophoresis (SMME)	188:226	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	2	64	theme	human	581:585	arg1	sera					587:590	pooled human sera	574:590	the pooled human sera fractionation by SMME	570:612	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	2	65	theme	mouse	506:510	arg1	fractionation					517:529	mouse sera fractionation	506:529	mouse sera fractionation	506:529	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	6	66	theme	profiling	1297:1305	arg1	differences					1307:1317	glycan profiling differences	1290:1317	glycan profiling differences between the bands of two samples	1290:1350	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	5	67	theme	Fut8-/-	1032:1038	arg1	mice					1040:1043	Fut8+/+ and Fut8-/- mice	1020:1043	Fut8+/+ and Fut8-/- mice	1020:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	8	68	theme	lectin	1703:1708	arg1	blotting					1710:1717	lectin blotting	1703:1717	lectin blotting of SMME-separated glycoproteins	1703:1749	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	2	69	theme	SMME	498:501	arg1	application					483:493	the application	479:493	the application of SMME to mouse sera fractionation	479:529	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	3	70	theme	acetone	757:763	arg1	solvent					765:771	acetone solvent	757:771	acetone solvent followed by heating	757:791	Furthermore, we have developed a fixation method for the lectin blotting of SMME-separated glycoproteins by immersing the SMME membranes into acetone solvent followed by heating.					
33421637	8	71	theme	profiling	1911:1919	arg1	differences					1921:1931	glycan profiling differences	1904:1931	glycan profiling differences between two samples in both research and clinical settings	1904:1990	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	7	72	theme	major	1566:1570	arg1	components					1580:1589	the major protein components	1562:1589	the major protein components of each fraction in mouse serum separated by SMME	1562:1639	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	8	73	from	patterns	1797:1804	arg1	mice					1854:1857	Fut8+/+ and Fut8-/- mice	1834:1857	Fut8+/+ and Fut8-/- mice	1834:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	6	74	theme	sera	1270:1273	arg1	fractionation					1275:1287	simple, rapid sera fractionation	1256:1287	simple, rapid sera fractionation	1256:1287	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	5	75	theme	proteins	1006:1013	arg1	patterns					988:995	glycosylation patterns	974:995	glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice	974:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	8	76	theme	glycoproteins	1737:1749	arg1	blotting					1710:1717	lectin blotting	1703:1717	lectin blotting of SMME-separated glycoproteins	1703:1749	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	6	77	theme	new	1358:1360	arg1	insight					1362:1368	a new insight	1356:1368	a new insight into the underlying mechanism that responsible for related diseases	1356:1436	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	5	78	theme	Fut8+/+	1020:1026	arg1	mice					1040:1043	Fut8+/+ and Fut8-/- mice	1020:1043	Fut8+/+ and Fut8-/- mice	1020:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	1	79	theme	separation	148:157	arg1	technique					159:167	a novel separation technique	140:167	a novel separation technique	140:167	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	2	80	theme	first	461:465	arg1	instance					467:474	the first instance	457:474	the first instance of the application of SMME to mouse sera fractionation	457:529	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	5	81	theme	sera	1219:1222	arg1	glycoproteins					1224:1236	mouse sera glycoproteins	1213:1236	mouse sera glycoproteins	1213:1236	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	0	82	theme	matrix	91:96	arg1	electrophoresis					98:112	supported molecular matrix electrophoresis	71:112	supported molecular matrix electrophoresis	71:112	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	1	83	theme	polyvinyl	324:332	arg1	alcohol					334:340	polyvinyl alcohol	324:340	polyvinyl alcohol	324:340	Previously, we developed a novel separation technique, namely, supported molecular matrix electrophoresis (SMME), which separates mucins on a PVDF membrane that impregnated with a hydrophilic polymer (such as polyvinyl alcohol), so it has the characteristics that are compatible with glycan analysis of the separated bands.					
33421637	5	84	from	mice	1040:1043	arg1	patterns					988:995	glycosylation patterns	974:995	glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice	974:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	5	84	from	mice	1040:1043	arg1	proteins					1006:1013	serum proteins	1000:1013	serum proteins from Fut8+/+ and Fut8-/- mice	1000:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	7	85	from	components	1580:1589	arg1	serum					1617:1621	mouse serum	1611:1621	mouse serum separated by SMME	1611:1639	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	7	86	theme	serum	1519:1523	arg1	proteins					1525:1532	mouse serum proteins	1513:1532	mouse serum proteins	1513:1532	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	2	87	theme	application	483:493	arg1	instance					467:474	the first instance	457:474	the first instance of the application of SMME to mouse sera fractionation	457:529	Here, we describe the first instance of the application of SMME to mouse sera fractionation and demonstrate their differences from the pooled human sera fractionation by SMME.					
33421637	8	88	theme	patterns	1797:1804	arg1	detection					1770:1778	the detection	1766:1778	the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice	1766:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	0	89	theme	glycosylation	6:18	arg1	analysis					20:27	Rapid glycosylation analysis	0:27	Rapid glycosylation analysis of mouse serum glycoproteins	0:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	8	90	theme	serum	1809:1813	arg1	glycoproteins					1815:1827	serum glycoproteins	1809:1827	serum glycoproteins from Fut8+/+ and Fut8-/- mice	1809:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	8	91	gly	glycosylation	1783:1795	arg1	glycoproteins					1815:1827	serum glycoproteins	1809:1827	serum glycoproteins from Fut8+/+ and Fut8-/- mice	1809:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	5	92	gly	α2,8-sialylated	1174:1188	arg1	O-glycans					1190:1198	α2,8-sialylated O-glycans	1174:1198	α2,8-sialylated O-glycans contained in mouse sera glycoproteins	1174:1236	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	6	93	theme	underlying	1379:1388	arg1	mechanism					1390:1398	the underlying mechanism	1375:1398	the underlying mechanism that responsible for related diseases	1375:1436	SMME can provide simple, rapid sera fractionation, glycan profiling differences between the bands of two samples and a new insight into the underlying mechanism that responsible for related diseases.					
33421637	7	94	theme	mouse	1513:1517	arg1	proteins					1525:1532	mouse serum proteins	1513:1532	mouse serum proteins	1513:1532	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	8	95	gly	glycoproteins	1815:1827	arg1	glycoproteins					1815:1827	serum glycoproteins	1809:1827	serum glycoproteins from Fut8+/+ and Fut8-/- mice	1809:1857	Furthermore, we successfully developed a fixation method for lectin blotting of SMME-separated glycoproteins and applied to the detection of glycosylation patterns of serum glycoproteins from Fut8+/+ and Fut8-/- mice, also, the method is promising for detecting glycan profiling differences between two samples in both research and clinical settings.					
33421637	5	96	theme	patterns	988:995	arg1	detection					961:969	the detection	957:969	the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice	957:1043	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	5	97	gly	glycoproteins	1224:1236	arg1	glycoproteins					1224:1236	mouse sera glycoproteins	1213:1236	mouse sera glycoproteins	1213:1236	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	5	98	theme	mass	1123:1126	arg1	spectrometry					1128:1139	mass spectrometry	1123:1139	mass spectrometry	1123:1139	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33421637	7	99	theme	first	1474:1478	arg1	application					1480:1490	the first application	1470:1490	the first application of SMME	1470:1498	SIGNIFICANCE: We describe that the first application of SMME can separate mouse serum proteins into six bands and identify the major protein components of each fraction in mouse serum separated by SMME.					
33421637	0	100	gly	glycoproteins	44:56	arg1	glycoproteins					44:56	mouse serum glycoproteins	32:56	mouse serum glycoproteins	32:56	Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis.					
33421637	5	101	theme	mouse	1213:1217	arg1	glycoproteins					1224:1236	mouse sera glycoproteins	1213:1236	mouse sera glycoproteins	1213:1236	We applied these techniques for the detection of glycosylation patterns of serum proteins from Fut8+/+ and Fut8-/- mice, further analyzed N-linked and O-linked glycans from the separated γ-bands by mass spectrometry, and demonstrated that there are α2,8-sialylated O-glycans contained in mouse sera glycoproteins.					
33755305	12	0	theme	protocol	1791:1798	arg1	development					1750:1760	development	1750:1760	development of a time and cost effective protocol for novel iPSF ECM production	1750:1828	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	10	1	theme	collagen	1509:1516	arg1	quantity					1518:1525	collagen quantity	1509:1525	collagen quantity	1509:1525	Interestingly, Carrageenan did not affect collagen quantity, but it significantly increased glycosaminoglycan deposition.					
33755305	8	2	with	combination	1208:1218	arg1	acid-over					1234:1242	ascorbic acid-over	1225:1242	ascorbic acid-over	1225:1242	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	2	3	theme	chronic	336:342	arg1	wounds					344:349	chronic wounds	336:349	chronic wounds	336:349	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	1	4	theme	crucial	254:260	arg1	role					262:265	a crucial role	252:265	a crucial role	252:265	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	1	5	theme	key	194:196	arg1	component					198:206	a key component	192:206	a key component of all tissues, including skin	192:237	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	1	5	theme	key	194:196	arg1	matrix					182:187	Extracellular matrix	168:187	Extracellular matrix	168:187	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	8	6	theme	ascorbic	1225:1232	arg1	acid-over					1234:1242	ascorbic acid-over	1225:1242	ascorbic acid-over	1225:1242	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	9	7	theme	matrix	1425:1430	arg1	composition					1432:1442	matrix composition	1425:1442	matrix composition	1425:1442	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	8	8	theme	molecular	1162:1170	arg1	crowders					1172:1179	two molecular crowders	1158:1179	two molecular crowders	1158:1179	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	4	9	theme	rich	612:615	arg1	source					595:600	a novel source	587:600	a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing	587:715	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	4	10	from	rich	612:615	arg1	components					637:646	pro-regenerative components	620:646	pro-regenerative components	620:646	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	5	11	theme	culturing	810:818	arg1	times					820:824	long cell culturing times	800:824	long cell culturing times which increases cost	800:845	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	6	12	theme	boosting	914:921	arg1	deposition					930:939	boosting matrix deposition	914:939	boosting matrix deposition	914:939	The aim of this work is to optimize the iPSF matrix production by boosting matrix deposition, without affecting its composition.					
33755305	5	13	dep	in	727:728	arg1	vitro					730:734	vitro	730:734	vitro	730:734	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	4	14	theme	pro-regenerative	620:635	arg1	components					637:646	pro-regenerative components	620:646	pro-regenerative components	620:646	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	0	15	theme	wound	153:157	arg1	healing					159:165	wound healing	153:165	wound healing	153:165	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	7	16	theme	good	979:982	arg1	technique					994:1002	A good candidate technique	977:1002	A good candidate technique to achieve this goal	977:1023	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	7	16	theme	good	979:982	arg1	crowding					1043:1050	macromolecular crowding	1028:1050	macromolecular crowding	1028:1050	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	8	17	theme	time	1266:1269	arg1	period					1256:1261	a prolonged period	1244:1261	a prolonged period of time	1244:1269	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	6	18	theme	iPSF	888:891	arg1	production					900:909	the iPSF matrix production	884:909	the iPSF matrix production by boosting matrix deposition	884:939	The aim of this work is to optimize the iPSF matrix production by boosting matrix deposition, without affecting its composition.					
33755305	4	19	theme	wound	703:707	arg1	healing					709:715	wound healing	703:715	wound healing	703:715	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	8	20	with	Ficoll	1182:1187	arg1	acid-over					1234:1242	ascorbic acid-over	1225:1242	ascorbic acid-over	1225:1242	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	9	21	with	combination	1282:1292	arg1	acid					1308:1311	ascorbic acid	1299:1311	ascorbic acid	1299:1311	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	0	22	theme	pluripotent	67:77	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	5	23	theme	long	800:803	arg1	times					820:824	long cell culturing times	800:824	long cell culturing times which increases cost	800:845	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	1	24	theme	Extracellular	168:180	arg1	component					198:206	a key component	192:206	a key component of all tissues, including skin	192:237	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	1	24	theme	Extracellular	168:180	arg1	matrix					182:187	Extracellular matrix	168:187	Extracellular matrix	168:187	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	8	25	with	crowders	1172:1179	arg1	acid-over					1234:1242	ascorbic acid-over	1225:1242	ascorbic acid-over	1225:1242	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	4	26	theme	scaffold	671:678	arg1	fabrication					680:690	scaffold fabrication	671:690	scaffold fabrication	671:690	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	0	27	theme	cell-derived	84:95	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	0	28	from	Optimization	0:11	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	5	29	theme	matrix	750:755	arg1	production					736:745	in vitro production	727:745	in vitro production of matrix by cells for scaffold fabrication	727:789	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	0	30	theme	stem	79:82	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	0	31	theme	extracellular	16:28	arg1	production					37:46	extracellular matrix production	16:46	extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts	16:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	4	32	theme	iPS	560:562	arg1	iPSF					571:574	iPSF	571:574	iPSF	571:574	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	4	32	theme	iPS	560:562	arg1	cells					564:568	iPS cells	560:568	iPS cells (iPSF)	560:575	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	2	33	theme	present	398:404	arg1	inflammation					365:376	inflammation	365:376	inflammation	365:376	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	3	34	theme	tissue	440:445	arg1	approaches					459:468	Many tissue engineering approaches	435:468	Many tissue engineering approaches for wound healing	435:486	Many tissue engineering approaches for wound healing provide a scaffold to mimic the native matrix.					
33755305	0	35	theme	production	37:46	arg1	Optimization					0:11	Optimization	0:11	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.	0:166	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	1	36	theme	wound	292:296	arg1	healing					298:304	wound healing	292:304	wound healing	292:304	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	12	37	theme	iPSF	1810:1813	arg1	production					1819:1828	novel iPSF ECM production	1804:1828	novel iPSF ECM production	1804:1828	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	11	38	from	matrix	1650:1655	arg1	scaffolds					1625:1633	scaffolds	1625:1633	scaffolds from harvested matrix	1625:1655	Finally, we successfully fabricated scaffolds from harvested matrix and confirmed their ability for cell growth and viability.					
33755305	0	39	theme	human	53:57	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	9	40	theme	matrix	1355:1360	arg1	weight					1366:1371	matrix dry weight	1355:1371	matrix dry weight	1355:1371	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	3	41	theme	wound	474:478	arg1	healing					480:486	wound healing	474:486	wound healing	474:486	Many tissue engineering approaches for wound healing provide a scaffold to mimic the native matrix.					
33755305	11	42	dep	cell	1689:1692	arg1	viability					1705:1713	viability	1705:1713	viability	1705:1713	Finally, we successfully fabricated scaffolds from harvested matrix and confirmed their ability for cell growth and viability.					
33755305	11	42	dep	cell	1689:1692	arg1	growth					1694:1699	growth	1694:1699	growth	1694:1699	Finally, we successfully fabricated scaffolds from harvested matrix and confirmed their ability for cell growth and viability.					
33755305	12	43	theme	engineering	1841:1851	arg1	scaffolds					1853:1861	tissue engineering scaffolds	1834:1861	tissue engineering scaffolds	1834:1861	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	2	44	from	present	398:404	arg1	wounds					427:432	these non-healing wounds	409:432	these non-healing wounds	409:432	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	0	45	theme	induced	59:65	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	0	46	from	application	138:148	arg1	healing					159:165	wound healing	153:165	wound healing	153:165	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	10	47	theme	glycosaminoglycan	1559:1575	arg1	deposition					1577:1586	glycosaminoglycan deposition	1559:1586	glycosaminoglycan deposition	1559:1586	Interestingly, Carrageenan did not affect collagen quantity, but it significantly increased glycosaminoglycan deposition.					
33755305	11	48	theme	harvested	1640:1648	arg1	matrix					1650:1655	harvested matrix	1640:1655	harvested matrix	1640:1655	Finally, we successfully fabricated scaffolds from harvested matrix and confirmed their ability for cell growth and viability.					
33755305	4	49	attach	derived	547:553	arg2	Fibroblasts					535:545	Fibroblasts	535:545	Fibroblasts derived from iPS cells (iPSF)	535:575	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	4	49	attach	derived	547:553	arg1	iPSF					571:574	iPSF	571:574	iPSF	571:574	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	4	49	attach	derived	547:553	arg1	cells					564:568	iPS cells	560:568	iPS cells (iPSF)	560:575	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	6	50	theme	work	864:867	arg1	aim					852:854	The aim	848:854	The aim of this work	848:867	The aim of this work is to optimize the iPSF matrix production by boosting matrix deposition, without affecting its composition.					
33755305	12	51	theme	novel	1804:1808	arg1	production					1819:1828	novel iPSF ECM production	1804:1828	novel iPSF ECM production	1804:1828	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	3	52	theme	native	520:525	arg1	matrix					527:532	the native matrix	516:532	the native matrix	516:532	Many tissue engineering approaches for wound healing provide a scaffold to mimic the native matrix.					
33755305	8	53	theme	Carrageenan-in	1193:1206	arg1	combination					1208:1218	Carrageenan-in combination	1193:1218	Carrageenan-in combination with ascorbic acid-over	1193:1242	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	4	54	from	components	637:646	arg1	rich					612:615	rich	612:615	rich	612:615	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	7	55	theme	macromolecular	1028:1041	arg1	technique					994:1002	A good candidate technique	977:1002	A good candidate technique to achieve this goal	977:1023	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	7	55	theme	macromolecular	1028:1041	arg1	crowding					1043:1050	macromolecular crowding	1028:1050	macromolecular crowding	1028:1050	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	5	56	theme	cell	805:808	arg1	times					820:824	long cell culturing times	800:824	long cell culturing times which increases cost	800:845	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	6	57	theme	matrix	923:928	arg1	deposition					930:939	boosting matrix deposition	914:939	boosting matrix deposition	914:939	The aim of this work is to optimize the iPSF matrix production by boosting matrix deposition, without affecting its composition.					
33755305	0	58	from	fibroblasts	97:107	arg1	Optimization					0:11	Optimization	0:11	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.	0:166	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	0	58	from	fibroblasts	97:107	arg1	production					37:46	extracellular matrix production	16:46	extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts	16:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	4	59	theme	matrix	605:610	arg1	source					595:600	a novel source	587:600	a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing	587:715	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	9	60	from	Ficoll	1272:1277	arg1	combination					1282:1292	combination	1282:1292	combination with ascorbic acid	1282:1311	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	8	61	theme	prolonged	1246:1254	arg1	period					1256:1261	a prolonged period	1244:1261	a prolonged period of time	1244:1269	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	7	62	theme	candidate	984:992	arg1	technique					994:1002	A good candidate technique	977:1002	A good candidate technique to achieve this goal	977:1023	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	7	62	theme	candidate	984:992	arg1	crowding					1043:1050	macromolecular crowding	1028:1050	macromolecular crowding	1028:1050	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	6	63	theme	matrix	893:898	arg1	production					900:909	the iPSF matrix production	884:909	the iPSF matrix production by boosting matrix deposition	884:939	The aim of this work is to optimize the iPSF matrix production by boosting matrix deposition, without affecting its composition.					
33755305	5	64	theme	in	727:728	arg1	production					736:745	in vitro production	727:745	in vitro production of matrix by cells for scaffold fabrication	727:789	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	2	65	from	wounds	427:432	arg1	present					398:404	present	398:404	present	398:404	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	0	66	theme	scaffold	113:120	arg1	fabrication					122:132	scaffold fabrication	113:132	scaffold fabrication for application in wound healing	113:165	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	12	67	theme	effective	1781:1789	arg1	protocol					1791:1798	a time and cost effective protocol	1765:1798	a time and cost effective protocol for novel iPSF ECM production	1765:1828	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	1	68	theme	tissues	215:221	arg1	component					198:206	a key component	192:206	a key component of all tissues, including skin	192:237	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	1	68	theme	tissues	215:221	arg1	matrix					182:187	Extracellular matrix	168:187	Extracellular matrix	168:187	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	12	69	dep	effective	1781:1789	arg1	time					1767:1770	time	1767:1770	time	1767:1770	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	3	70	theme	Many	435:438	arg1	approaches					459:468	Many tissue engineering approaches	435:468	Many tissue engineering approaches for wound healing	435:486	Many tissue engineering approaches for wound healing provide a scaffold to mimic the native matrix.					
33755305	5	71	theme	scaffold	770:777	arg1	fabrication					779:789	scaffold fabrication	770:789	scaffold fabrication	770:789	However, in vitro production of matrix by cells for scaffold fabrication requires long cell culturing times which increases cost.					
33755305	9	72	theme	ascorbic	1299:1306	arg1	acid					1308:1311	ascorbic acid	1299:1311	ascorbic acid	1299:1311	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	2	73	theme	chronic	357:363	arg1	inflammation					365:376	inflammation	365:376	inflammation	365:376	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	0	74	theme	matrix	30:35	arg1	production					37:46	extracellular matrix production	16:46	extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts	16:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	1	75	theme	complex	274:280	arg1	events					282:287	the complex events	270:287	the complex events of wound healing	270:304	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	4	76	used	used	662:665	arg2	components					637:646	pro-regenerative components	620:646	pro-regenerative components	620:646	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	2	77	theme	non-healing	415:425	arg1	wounds					427:432	these non-healing wounds	409:432	these non-healing wounds	409:432	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	7	78	theme	mature	1110:1115	arg1	collagen					1117:1124	mature collagen	1110:1124	mature collagen	1110:1124	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	9	79	theme	collagen	1331:1338	arg1	deposition					1340:1349	collagen deposition	1331:1349	collagen deposition	1331:1349	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	8	80	dep	crowders	1172:1179	arg1	period					1256:1261	a prolonged period	1244:1261	a prolonged period of time	1244:1269	We tested two molecular crowders, Ficoll and Carrageenan-in combination with ascorbic acid-over a prolonged period of time.					
33755305	3	81	theme	engineering	447:457	arg1	approaches					459:468	Many tissue engineering approaches	435:468	Many tissue engineering approaches for wound healing	435:486	Many tissue engineering approaches for wound healing provide a scaffold to mimic the native matrix.					
33755305	7	82	theme	procollagen	1093:1103	arg1	conversion					1079:1088	conversion	1079:1088	conversion of procollagen into mature collagen and its accumulation	1079:1145	A good candidate technique to achieve this goal is macromolecular crowding, which is known to promote conversion of procollagen into mature collagen and its accumulation.					
33755305	1	83	theme	healing	298:304	arg1	events					282:287	the complex events	270:287	the complex events of wound healing	270:304	Extracellular matrix is a key component of all tissues, including skin and it plays a crucial role in the complex events of wound healing.					
33755305	12	84	theme	tissue	1834:1839	arg1	engineering					1841:1851	tissue engineering	1834:1851	tissue engineering scaffolds	1834:1861	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	2	85	attach	present	398:404	arg1	wounds					427:432	these non-healing wounds	409:432	these non-healing wounds	409:432	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	2	85	attach	present	398:404	arg2	inflammation					365:376	inflammation	365:376	inflammation	365:376	These events are impaired in chronic wounds, with chronic inflammation and infection often present in these non-healing wounds.					
33755305	0	86	link	cell-derived	84:95	arg1	fibroblasts					97:107	human induced pluripotent stem cell-derived fibroblasts	53:107	human induced pluripotent stem cell-derived fibroblasts	53:107	Optimization of extracellular matrix production from human induced pluripotent stem cell-derived fibroblasts for scaffold fabrication for application in wound healing.					
33755305	9	87	theme	dry	1362:1364	arg1	weight					1366:1371	matrix dry weight	1355:1371	matrix dry weight	1355:1371	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
33755305	12	88	theme	ECM	1815:1817	arg1	production					1819:1828	novel iPSF ECM production	1804:1828	novel iPSF ECM production	1804:1828	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	4	89	theme	novel	589:593	arg1	source					595:600	a novel source	587:600	a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing	587:715	Fibroblasts derived from iPS cells (iPSF) represent a novel source of matrix rich in pro-regenerative components, which can be used for scaffold fabrication to improve wound healing.					
33755305	12	90	theme	cost	1776:1779	arg1	protocol					1791:1798	a time and cost effective protocol	1765:1798	a time and cost effective protocol for novel iPSF ECM production	1765:1828	This work lays the foundation for development of a time and cost effective protocol for novel iPSF ECM production for tissue engineering scaffolds.					
33755305	9	91	theme	ascorbic	1385:1392	arg1	acid					1394:1397	ascorbic acid	1385:1397	ascorbic acid alone	1385:1403	Ficoll in combination with ascorbic acid notably increased collagen deposition and matrix dry weight compared to ascorbic acid alone, and did not affect matrix composition as measured by RT-PCR.					
32636343	9	0	theme	GPI-anchored	1627:1638	arg1	PdBG2					1640:1644	GPI-anchored PdBG2	1627:1644	GPI-anchored PdBG2	1627:1644	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	6	1	theme	modified	1107:1114	arg1	contents					1119:1126	genetically modified SL contents	1095:1126	genetically modified SL contents using two independent SL pathway mutants	1095:1167	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	2	2	theme	intercellular	375:387	arg1	trafficking					389:399	the intercellular trafficking	371:399	the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins	371:458	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	6	3	theme	several	1051:1057	arg1	inhibitors					1080:1089	several potential SL pathway inhibitors	1051:1089	several potential SL pathway inhibitors	1051:1089	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	8	4	theme	PdBG2	1422:1426	arg1	protein					1428:1434	the GPI-anchored PdBG2 protein	1405:1434	the GPI-anchored PdBG2 protein	1405:1434	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	9	5	theme	PD	1572:1573	arg1	homeostasis					1583:1593	PD callose homeostasis	1572:1593	PD callose homeostasis	1572:1593	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	3	6	attach	presence	512:519	arg1	PD					535:536	PD	535:536	PD	535:536	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	3	6	attach	presence	512:519	arg2	callose					524:530	callose	524:530	callose	524:530	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	4	7	theme	protein	735:741	arg1	trafficking					743:753	membrane protein trafficking	726:753	membrane protein trafficking	726:753	SLs are implicated in signaling and membrane protein trafficking; however, the underlying processes linking SL composition to the control of symplasmic apertures remain unknown.					
32636343	9	8	theme	lipid	1530:1534	arg1	rafts					1536:1540	SL-enriched lipid rafts	1518:1540	SL-enriched lipid rafts	1518:1540	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	6	9	theme	pathway	1153:1159	arg1	mutants					1161:1167	two independent SL pathway mutants	1134:1167	two independent SL pathway mutants	1134:1167	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	5	10	theme	symplasmic	977:986	arg1	apertures					988:996	symplasmic apertures	977:996	symplasmic apertures in Arabidopsis (Arabidopsis thaliana)	977:1034	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	1	11	contain	contain	204:210	arg1	membranes					128:136	Plasma membranes	121:136	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD)	121:202	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	1	11	contain	contain	204:210	arg2	rafts					227:231	abundant lipid rafts	212:231	abundant lipid rafts	212:231	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	5	12	theme	Arabidopsis	1014:1024	arg1	thaliana					1026:1033	Arabidopsis (Arabidopsis thaliana)	1001:1034	Arabidopsis (Arabidopsis thaliana)	1001:1034	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	10	13	theme	proteins	1788:1795	arg1	trafficking					1735:1745	intracellular trafficking	1721:1745	intracellular trafficking	1721:1745	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	10	13	theme	proteins	1788:1795	arg1	targeting					1759:1767	precise targeting	1751:1767	precise targeting	1751:1767	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	3	14	theme	callose	582:588	arg1	accumulation					590:601	callose accumulation	582:601	callose accumulation	582:601	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	2	15	theme	lipid	347:351	arg1	integrity					358:366	lipid raft integrity	347:366	lipid raft integrity	347:366	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	10	16	theme	molecular	1692:1700	arg1	linkage					1702:1708	the molecular linkage	1688:1708	the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs	1688:1822	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	5	17	from	apertures	988:996	arg1	thaliana					1026:1033	Arabidopsis (Arabidopsis thaliana)	1001:1034	Arabidopsis (Arabidopsis thaliana)	1001:1034	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	2	18	theme	sterols	304:310	arg1	Reduction					291:299	Reduction	291:299	Reduction of sterols	291:310	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	10	19	theme	glucosyl	1811:1818	arg1	SLs					1820:1822	glucosyl SLs	1811:1822	glucosyl SLs	1811:1822	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	1	20	theme	abundant	212:219	arg1	rafts					227:231	abundant lipid rafts	212:231	abundant lipid rafts	212:231	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	7	21	theme	callose	1195:1201	arg1	deposition					1203:1212	callose deposition	1195:1212	callose deposition	1195:1212	We were able to modulate callose deposition to control symplasmic connectivity through perturbations of SL metabolism.					
32636343	8	22	from	Alteration	1289:1298	arg1	glucosylhydroxyceramides					1303:1326	glucosylhydroxyceramides	1303:1326	glucosylhydroxyceramides	1303:1326	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	9	23	theme	SL-enriched	1518:1528	arg1	rafts					1536:1540	SL-enriched lipid rafts	1518:1540	SL-enriched lipid rafts	1518:1540	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	9	24	theme	PdBG2	1640:1644	arg1	targeting					1614:1622	the targeting	1610:1622	the targeting of GPI-anchored PdBG2	1610:1644	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	1	25	theme	Plasma	121:126	arg1	membranes					128:136	Plasma membranes	121:136	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD)	121:202	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	6	26	theme	SL	1150:1151	arg1	mutants					1161:1167	two independent SL pathway mutants	1134:1167	two independent SL pathway mutants	1134:1167	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	1	27	theme	lipid	221:225	arg1	rafts					227:231	abundant lipid rafts	212:231	abundant lipid rafts	212:231	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	2	28	theme	callose	487:493	arg1	enhancement					495:505	callose enhancement	487:505	callose enhancement	487:505	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	6	29	theme	independent	1138:1148	arg1	mutants					1161:1167	two independent SL pathway mutants	1134:1167	two independent SL pathway mutants	1134:1167	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	3	30	theme	callose	607:613	arg1	degradation					615:625	callose degradation	607:625	callose degradation	607:625	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	8	31	theme	related	1331:1337	arg1	composition					1342:1352	related SL composition	1331:1352	related SL composition	1331:1352	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	8	32	from	composition	1342:1352	arg1	glucosylhydroxyceramides					1303:1326	glucosylhydroxyceramides	1303:1326	glucosylhydroxyceramides	1303:1326	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	3	33	theme	degradation	615:625	arg1	regulation					568:577	the regulation	564:577	the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively	564:687	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	8	34	theme	SL	1339:1340	arg1	composition					1342:1352	related SL composition	1331:1352	related SL composition	1331:1352	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	7	35	theme	metabolism	1277:1286	arg1	perturbations					1257:1269	perturbations	1257:1269	perturbations of SL metabolism	1257:1286	We were able to modulate callose deposition to control symplasmic connectivity through perturbations of SL metabolism.					
32636343	8	36	theme	GPI-anchored	1409:1420	arg1	protein					1428:1434	the GPI-anchored PdBG2 protein	1405:1434	the GPI-anchored PdBG2 protein	1405:1434	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	3	37	from	PD	535:536	arg1	presence					512:519	The presence	508:519	The presence of callose at PD	508:536	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	3	38	theme	accumulation	590:601	arg1	regulation					568:577	the regulation	564:577	the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively	564:687	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	9	39	theme	symplasmic	1547:1556	arg1	channeling					1558:1567	symplasmic channeling	1547:1567	symplasmic channeling to PD callose homeostasis	1547:1593	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	6	40	theme	SL	1116:1117	arg1	contents					1119:1126	genetically modified SL contents	1095:1126	genetically modified SL contents using two independent SL pathway mutants	1095:1167	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	4	41	theme	underlying	769:778	arg1	processes					780:788	the underlying processes	765:788	the underlying processes linking SL composition to the control of symplasmic apertures	765:850	SLs are implicated in signaling and membrane protein trafficking; however, the underlying processes linking SL composition to the control of symplasmic apertures remain unknown.					
32636343	5	42	theme	Arabidopsis	1001:1011	arg1	thaliana					1026:1033	Arabidopsis (Arabidopsis thaliana)	1001:1034	Arabidopsis (Arabidopsis thaliana)	1001:1034	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	2	43	theme	proteins	451:458	arg1	trafficking					389:399	the intercellular trafficking	371:399	the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins	371:458	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	0	44	theme	Plasmodesmata	74:86	arg1	Proteins					88:95	Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins	36:95	Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins	36:95	Sphingolipids Modulate Secretion of Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins and Callose Deposition.					
32636343	5	45	theme	SLs	888:890	arg1	SLs					888:890	SLs	888:890	SLs in plants	888:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	5	45	theme	SLs	888:890	arg1	variety					877:883	The wide variety	868:883	The wide variety of SLs in plants	868:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	5	46	theme	SL	935:936	arg1	molecules					938:946	SL molecules	935:946	SL molecules	935:946	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	2	47	theme	PD	448:449	arg1	proteins					451:458	glycosylphosphatidylinositol (GPI)-anchored PD proteins	404:458	glycosylphosphatidylinositol (GPI)-anchored PD proteins	404:458	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	0	48	theme	Glycosylphosphatidylinositol-Anchored	36:72	arg1	Proteins					88:95	Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins	36:95	Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins	36:95	Sphingolipids Modulate Secretion of Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins and Callose Deposition.					
32636343	10	49	theme	linkage	1702:1708	arg1	understanding					1671:1683	our understanding	1667:1683	our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs	1667:1822	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	8	50	theme	secretory	1381:1389	arg1	machinery					1391:1399	the secretory machinery	1377:1399	the secretory machinery for the GPI-anchored PdBG2 protein	1377:1434	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	5	51	from	SLs	888:890	arg1	plants					895:900	plants	895:900	plants	895:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	2	52	theme	-anchored	438:446	arg1	proteins					451:458	glycosylphosphatidylinositol (GPI)-anchored PD proteins	404:458	glycosylphosphatidylinositol (GPI)-anchored PD proteins	404:458	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	7	53	theme	SL	1274:1275	arg1	metabolism					1277:1286	SL metabolism	1274:1286	SL metabolism	1274:1286	We were able to modulate callose deposition to control symplasmic connectivity through perturbations of SL metabolism.					
32636343	10	54	theme	intracellular	1721:1733	arg1	trafficking					1735:1745	intracellular trafficking	1721:1745	intracellular trafficking	1721:1745	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	8	55	theme	callose	1473:1479	arg1	overaccumulation					1453:1468	an overaccumulation	1450:1468	an overaccumulation of callose	1450:1479	Alteration in glucosylhydroxyceramides or related SL composition particularly disturbed the secretory machinery for the GPI-anchored PdBG2 protein, resulting in an overaccumulation of callose.					
32636343	1	56	theme	symplasmic	158:167	arg1	nanochannels					169:180	the symplasmic nanochannels	154:180	the symplasmic nanochannels of plasmodesmata (PD)	154:202	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	0	57	theme	Proteins	88:95	arg1	Secretion					23:31	Secretion	23:31	Secretion of Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins	23:95	Sphingolipids Modulate Secretion of Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins and Callose Deposition.					
32636343	0	57	theme	Proteins	88:95	arg1	Deposition					109:118	Callose Deposition	101:118	Callose Deposition	101:118	Sphingolipids Modulate Secretion of Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins and Callose Deposition.					
32636343	3	58	theme	callose	630:636	arg1	synthases					638:646	callose synthases	630:646	callose synthases	630:646	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	3	58	theme	callose	630:636	arg1	BGs					670:672	BGs	670:672	BGs	670:672	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	5	59	from	variety	877:883	arg1	plants					895:900	plants	895:900	plants	895:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	10	60	theme	PD	1785:1786	arg1	proteins					1788:1795	GPI-anchored PD proteins	1772:1795	GPI-anchored PD proteins incorporating glucosyl SLs	1772:1822	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	4	61	theme	symplasmic	831:840	arg1	apertures					842:850	symplasmic apertures	831:850	symplasmic apertures	831:850	SLs are implicated in signaling and membrane protein trafficking; however, the underlying processes linking SL composition to the control of symplasmic apertures remain unknown.					
32636343	2	62	theme	raft	353:356	arg1	integrity					358:366	lipid raft integrity	347:366	lipid raft integrity	347:366	Reduction of sterols has highlighted the role played by lipid raft integrity in the intercellular trafficking of glycosylphosphatidylinositol (GPI)-anchored PD proteins, particularly in affecting callose enhancement.					
32636343	4	63	theme	SL	798:799	arg1	composition					801:811	SL composition	798:811	SL composition	798:811	SLs are implicated in signaling and membrane protein trafficking; however, the underlying processes linking SL composition to the control of symplasmic apertures remain unknown.					
32636343	5	64	theme	wide	872:875	arg1	SLs					888:890	SLs	888:890	SLs in plants	888:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	5	64	theme	wide	872:875	arg1	variety					877:883	The wide variety	868:883	The wide variety of SLs in plants	868:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	1	65	theme	plasmodesmata	185:197	arg1	nanochannels					169:180	the symplasmic nanochannels	154:180	the symplasmic nanochannels of plasmodesmata (PD)	154:202	Plasma membranes encapsulated in the symplasmic nanochannels of plasmodesmata (PD) contain abundant lipid rafts, which are enriched with sphingolipids (SLs) and sterols.					
32636343	6	66	theme	pathway	1072:1078	arg1	inhibitors					1080:1089	several potential SL pathway inhibitors	1051:1089	several potential SL pathway inhibitors	1051:1089	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	10	67	theme	precise	1751:1757	arg1	targeting					1759:1767	precise targeting	1751:1767	precise targeting	1751:1767	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	9	68	theme	callose	1575:1581	arg1	homeostasis					1583:1593	PD callose homeostasis	1572:1593	PD callose homeostasis	1572:1593	Moreover, our results revealed that SL-enriched lipid rafts link symplasmic channeling to PD callose homeostasis by controlling the targeting of GPI-anchored PdBG2.					
32636343	4	69	theme	membrane	726:733	arg1	trafficking					743:753	membrane protein trafficking	726:753	membrane protein trafficking	726:753	SLs are implicated in signaling and membrane protein trafficking; however, the underlying processes linking SL composition to the control of symplasmic apertures remain unknown.					
32636343	3	70	theme	callose	524:530	arg1	presence					512:519	The presence	508:519	The presence of callose at PD	508:536	The presence of callose at PD is strongly attributed to the regulation of callose accumulation and callose degradation by callose synthases and β-1,3-glucanases (BGs), respectively.					
32636343	6	71	theme	SL	1069:1070	arg1	inhibitors					1080:1089	several potential SL pathway inhibitors	1051:1089	several potential SL pathway inhibitors	1051:1089	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
32636343	0	72	theme	Callose	101:107	arg1	Deposition					109:118	Callose Deposition	101:118	Callose Deposition	101:118	Sphingolipids Modulate Secretion of Glycosylphosphatidylinositol-Anchored Plasmodesmata Proteins and Callose Deposition.					
32636343	4	73	theme	apertures	842:850	arg1	control					820:826	the control	816:826	the control of symplasmic apertures	816:850	SLs are implicated in signaling and membrane protein trafficking; however, the underlying processes linking SL composition to the control of symplasmic apertures remain unknown.					
32636343	5	74	from	plants	895:900	arg1	SLs					888:890	SLs	888:890	SLs in plants	888:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	5	74	from	plants	895:900	arg1	variety					877:883	The wide variety	868:883	The wide variety of SLs in plants	868:900	The wide variety of SLs in plants prompted us to investigate which SL molecules are important for regulating symplasmic apertures in Arabidopsis (Arabidopsis thaliana).					
32636343	7	75	theme	symplasmic	1225:1234	arg1	connectivity					1236:1247	symplasmic connectivity	1225:1247	symplasmic connectivity	1225:1247	We were able to modulate callose deposition to control symplasmic connectivity through perturbations of SL metabolism.					
32636343	10	76	theme	GPI-anchored	1772:1783	arg1	proteins					1788:1795	GPI-anchored PD proteins	1772:1795	GPI-anchored PD proteins incorporating glucosyl SLs	1772:1822	This study elevates our understanding of the molecular linkage underlying intracellular trafficking and precise targeting of GPI-anchored PD proteins incorporating glucosyl SLs.					
32636343	6	77	theme	potential	1059:1067	arg1	inhibitors					1080:1089	several potential SL pathway inhibitors	1051:1089	several potential SL pathway inhibitors	1051:1089	We introduced several potential SL pathway inhibitors and genetically modified SL contents using two independent SL pathway mutants.					
34324907	10	0	theme	wider	1462:1466	arg1	application					1468:1478	wider application	1462:1478	wider application of this biopolymer	1462:1497	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	10	1	theme	improving	1360:1368	arg1	yields					1374:1379	improving BNC yields	1360:1379	improving BNC yields	1360:1379	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	0	2	theme	properties	83:92	arg1	differentiation					64:78	differentiation	64:78	differentiation	64:78	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	0	2	theme	properties	83:92	arg1	yield					54:58	yield	54:58	yield	54:58	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	3	3	from	increase	497:504	arg1	yield					521:525	the yield	517:525	the yield	517:525	Optimization resulted in an increase of 470% in the yield, compared to the Schramm-Hestrin medium.					
34324907	8	4	from	decrease	1068:1075	arg1	crystallinity					1080:1092	crystallinity	1080:1092	crystallinity	1080:1092	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	0	5	theme	cellulose	107:115	arg1	properties					83:92	properties	83:92	properties of bacterial cellulose	83:115	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	9	6	theme	young	1223:1227	arg1	modulus					1229:1235	young modulus	1223:1235	young modulus	1223:1235	The stress, strain, young modulus and toughness were improved 2.8-4.2 times, 1-1.9 times, 2.4-3.5 times and 2.5-6.8 times, respectively.					
34324907	4	7	theme	growth	576:581	arg1	generation					631:640	by-products generation	619:640	by-products generation	619:640	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	4	7	theme	growth	576:581	arg1	profiles					583:590	Culture growth profiles	568:590	Culture growth profiles	568:590	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	4	7	theme	growth	576:581	arg1	consumption					603:613	substrate consumption	593:613	substrate consumption	593:613	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	0	8	theme	bacterial	97:105	arg1	cellulose					107:115	bacterial cellulose	97:115	bacterial cellulose	97:115	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	10	9	theme	new	1344:1346	arg1	approach					1348:1355	The new approach	1340:1355	The new approach to improving BNC yields and properties presented here	1340:1409	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	3	10	dep	resulted	482:489	arg1	compared					528:535	compared	528:535	compared to the Schramm-Hestrin medium	528:565	Optimization resulted in an increase of 470% in the yield, compared to the Schramm-Hestrin medium.					
34324907	4	11	theme	Culture	568:574	arg1	generation					631:640	by-products generation	619:640	by-products generation	619:640	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	4	11	theme	Culture	568:574	arg1	profiles					583:590	Culture growth profiles	568:590	Culture growth profiles	568:590	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	4	11	theme	Culture	568:574	arg1	consumption					603:613	substrate consumption	593:613	substrate consumption	593:613	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	0	12	theme	metabolic	120:128	arg1	enhancers					130:138	metabolic enhancers	120:138	metabolic enhancers	120:138	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	2	13	dep	yield	428:432	arg1	the					424:426	the	424:426	the	424:426	Both ethanol and lactic acid, when added separately or jointly, affected the yield and properties of the biomaterial.					
34324907	8	14	theme	Iα	1178:1179	arg1	ratio					1159:1163	the ratio	1155:1163	the ratio of cellulose Iα	1155:1179	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	8	14	theme	Iα	1178:1179	arg1	unchanged					1192:1200	unchanged	1192:1200	unchanged	1192:1200	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	6	15	theme	BNC	863:865	arg1	crystallinity					891:903	crystallinity	891:903	crystallinity	891:903	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	6	15	theme	BNC	863:865	arg1	strength					941:948	tensile strength	933:948	tensile strength	933:948	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	6	15	theme	BNC	863:865	arg1	structure					880:888	the structure	876:888	the structure	876:888	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	6	15	theme	BNC	863:865	arg1	capacity					920:927	water holding capacity	906:927	water holding capacity	906:927	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	6	15	theme	BNC	863:865	arg1	properties					849:858	The properties	845:858	The properties	845:858	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	5	16	theme	gluconic	789:796	arg1	acid					798:801	gluconic acid	789:801	gluconic acid	789:801	The growth rate was increased for cultures supplemented with lactic acid and both lactic acid and ethanol, while the production of gluconic acid was diminished for all modified cultures.					
34324907	8	17	theme	cellulose	1168:1176	arg1	Iα					1178:1179	cellulose Iα	1168:1179	cellulose Iα	1168:1179	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	0	18	theme	surface	9:15	arg1	improvement					35:45	Response surface methodology-based improvement	0:45	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.	0:139	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	7	19	theme	wider	1044:1048	arg1	fibers					1050:1055	wider fibers	1044:1055	wider fibers	1044:1055	BNC produced in optimal conditions is more porous and characterized by wider fibers.					
34324907	9	20	theme	improved	1256:1263	arg1	times					1273:1277	2.8-4.2 times	1265:1277	2.8-4.2 times	1265:1277	The stress, strain, young modulus and toughness were improved 2.8-4.2 times, 1-1.9 times, 2.4-3.5 times and 2.5-6.8 times, respectively.					
34324907	9	20	theme	improved	1256:1263	arg1	stress					1207:1212	stress	1207:1212	stress	1207:1212	The stress, strain, young modulus and toughness were improved 2.8-4.2 times, 1-1.9 times, 2.4-3.5 times and 2.5-6.8 times, respectively.					
34324907	1	21	theme	ethanol	182:188	arg1	effect					172:177	the effect	168:177	the effect of ethanol and lactic acid on the production of bacterial cellulose	168:245	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	0	22	theme	Response	0:7	arg1	improvement					35:45	Response surface methodology-based improvement	0:45	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.	0:139	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	1	23	theme	optimal	266:272	arg1	composition					274:284	the optimal composition	262:284	the optimal composition of a co-supplemented culture using response surface methodology	262:348	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	10	24	theme	BNC	1370:1372	arg1	yields					1374:1379	improving BNC yields	1360:1379	improving BNC yields	1360:1379	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	3	25	theme	Schramm-Hestrin	544:558	arg1	medium					560:565	the Schramm-Hestrin medium	540:565	the Schramm-Hestrin medium	540:565	Optimization resulted in an increase of 470% in the yield, compared to the Schramm-Hestrin medium.					
34324907	5	26	theme	lactic	719:724	arg1	acid					726:729	lactic acid	719:729	lactic acid	719:729	The growth rate was increased for cultures supplemented with lactic acid and both lactic acid and ethanol, while the production of gluconic acid was diminished for all modified cultures.					
34324907	5	27	theme	acid	798:801	arg1	production					775:784	the production	771:784	the production of gluconic acid	771:801	The growth rate was increased for cultures supplemented with lactic acid and both lactic acid and ethanol, while the production of gluconic acid was diminished for all modified cultures.					
34324907	6	28	theme	tensile	933:939	arg1	strength					941:948	tensile strength	933:948	tensile strength	933:948	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	8	29	theme	additives	1144:1152	arg1	addition					1102:1109	the addition	1098:1109	the addition of ethanol, lactic acid and both additives	1098:1152	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	1	30	theme	lactic	194:199	arg1	acid					201:204	lactic acid	194:204	lactic acid	194:204	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	0	31	theme	methodology-based	17:33	arg1	improvement					35:45	Response surface methodology-based improvement	0:45	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.	0:139	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	1	32	theme	acid	201:204	arg1	effect					172:177	the effect	168:177	the effect of ethanol and lactic acid on the production of bacterial cellulose	168:245	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	10	33	theme	biopolymer	1488:1497	arg1	application					1468:1478	wider application	1462:1478	wider application of this biopolymer	1462:1497	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	10	33	theme	biopolymer	1488:1497	arg1	production					1447:1456	more economical production	1431:1456	more economical production	1431:1456	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	2	34	theme	biomaterial	456:466	arg1	properties					438:447	properties	438:447	properties	438:447	Both ethanol and lactic acid, when added separately or jointly, affected the yield and properties of the biomaterial.					
34324907	2	34	theme	biomaterial	456:466	arg1	yield					428:432	yield	428:432	yield	428:432	Both ethanol and lactic acid, when added separately or jointly, affected the yield and properties of the biomaterial.					
34324907	1	35	theme	co-supplemented	291:305	arg1	culture					307:313	a co-supplemented culture	289:313	a co-supplemented culture using response surface methodology	289:348	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	0	36	dep	yield	54:58	arg1	the					50:52	the	50:52	the	50:52	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	5	37	theme	modified	826:833	arg1	cultures					835:842	all modified cultures	822:842	all modified cultures	822:842	The growth rate was increased for cultures supplemented with lactic acid and both lactic acid and ethanol, while the production of gluconic acid was diminished for all modified cultures.					
34324907	1	38	theme	culture	307:313	arg1	composition					274:284	the optimal composition	262:284	the optimal composition of a co-supplemented culture using response surface methodology	262:348	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	5	39	theme	growth	662:667	arg1	rate					669:672	The growth rate	658:672	The growth rate	658:672	The growth rate was increased for cultures supplemented with lactic acid and both lactic acid and ethanol, while the production of gluconic acid was diminished for all modified cultures.					
34324907	7	40	theme	optimal	989:995	arg1	conditions					997:1006	optimal conditions	989:1006	optimal conditions	989:1006	BNC produced in optimal conditions is more porous and characterized by wider fibers.					
34324907	0	41	theme	yield	54:58	arg1	improvement					35:45	Response surface methodology-based improvement	0:45	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.	0:139	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	10	42	theme	economical	1436:1445	arg1	production					1447:1456	more economical production	1431:1456	more economical production	1431:1456	The new approach to improving BNC yields and properties presented here could contribute to more economical production and wider application of this biopolymer.					
34324907	1	43	theme	response	321:328	arg1	methodology					338:348	response surface methodology	321:348	response surface methodology	321:348	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	6	44	theme	holding	912:918	arg1	capacity					920:927	water holding capacity	906:927	water holding capacity	906:927	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	8	45	theme	ethanol	1114:1120	arg1	addition					1102:1109	the addition	1098:1109	the addition of ethanol, lactic acid and both additives	1098:1152	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	8	46	theme	lactic	1123:1128	arg1	acid					1130:1133	lactic acid	1123:1133	lactic acid	1123:1133	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	8	47	theme	acid	1130:1133	arg1	addition					1102:1109	the addition	1098:1109	the addition of ethanol, lactic acid and both additives	1098:1152	Despite a decrease in crystallinity, by the addition of ethanol, lactic acid and both additives, the ratio of cellulose Iα was almost unchanged.					
34324907	3	48	theme	%	512:512	arg1	increase					497:504	an increase	494:504	an increase of 470% in the yield	494:525	Optimization resulted in an increase of 470% in the yield, compared to the Schramm-Hestrin medium.					
34324907	5	49	theme	lactic	740:745	arg1	acid					747:750	lactic acid	740:750	lactic acid	740:750	The growth rate was increased for cultures supplemented with lactic acid and both lactic acid and ethanol, while the production of gluconic acid was diminished for all modified cultures.					
34324907	4	50	theme	by-products	619:629	arg1	generation					631:640	by-products generation	619:640	by-products generation	619:640	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	4	50	theme	by-products	619:629	arg1	profiles					583:590	Culture growth profiles	568:590	Culture growth profiles	568:590	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	1	51	theme	bacterial	227:235	arg1	cellulose					237:245	bacterial cellulose	227:245	bacterial cellulose	227:245	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	0	52	theme	differentiation	64:78	arg1	improvement					35:45	Response surface methodology-based improvement	0:45	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.	0:139	Response surface methodology-based improvement of the yield and differentiation of properties of bacterial cellulose by metabolic enhancers.					
34324907	1	53	theme	surface	330:336	arg1	methodology					338:348	response surface methodology	321:348	response surface methodology	321:348	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	4	54	theme	substrate	593:601	arg1	profiles					583:590	Culture growth profiles	568:590	Culture growth profiles	568:590	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	4	54	theme	substrate	593:601	arg1	consumption					603:613	substrate consumption	593:613	substrate consumption	593:613	Culture growth profiles, substrate consumption and by-products generation, were examined.					
34324907	1	55	from	effect	172:177	arg1	production					213:222	the production	209:222	the production of bacterial cellulose	209:245	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34324907	2	56	theme	lactic	368:373	arg1	acid					375:378	lactic acid	368:378	lactic acid	368:378	Both ethanol and lactic acid, when added separately or jointly, affected the yield and properties of the biomaterial.					
34324907	6	57	theme	water	906:910	arg1	capacity					920:927	water holding capacity	906:927	water holding capacity	906:927	The properties of BNC, such as the structure, crystallinity, water holding capacity and tensile strength, were also determined.					
34324907	1	58	theme	cellulose	237:245	arg1	production					213:222	the production	209:222	the production of bacterial cellulose	209:245	This study aims to examine the effect of ethanol and lactic acid on the production of bacterial cellulose, and determine the optimal composition of a co-supplemented culture using response surface methodology.					
34364607	7	0	from	PPy/Ag	1467:1472	arg1	fabric					1503:1508	alginate fabric	1494:1508	alginate fabric	1494:1508	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	3	1	theme	10 h.	638:642	arg1	time					630:633	the reaction time	617:633	the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively	617:955	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	2	theme	Raman	884:888	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	2	theme	Raman	884:888	arg1	spectroscopy					890:901	Raman spectroscopy	884:901	Raman spectroscopy	884:901	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	1	3	theme	polypyrrole/silver	153:170	arg1	composite					181:189	the polypyrrole/silver (PPy/Ag) composite	149:189	the polypyrrole/silver (PPy/Ag) composite	149:189	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	3	4	theme	X-ray	806:810	arg1	XRD					838:840	XRD	838:840	XRD	838:840	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	4	theme	X-ray	806:810	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	4	theme	X-ray	806:810	arg1	spectroscopy					824:835	X-ray diffraction spectroscopy	806:835	X-ray diffraction spectroscopy (XRD)	806:841	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	4	theme	X-ray	806:810	arg1	TGA					938:940	TGA	938:940	TGA	938:940	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	1	5	theme	oxidative	250:258	arg1	polymerization					260:273	chemical oxidative polymerization	241:273	chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant	241:376	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	2	6	theme	time	439:442	arg1	effects					383:389	The effects	379:389	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite	379:506	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	1	7	theme	sodium	335:340	arg1	SDS					359:361	SDS	359:361	SDS	359:361	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	1	7	theme	sodium	335:340	arg1	sulfate					350:356	sodium dodecyl sulfate	335:356	sodium dodecyl sulfate (SDS)	335:362	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	7	8	dep	it	1518:1519	arg1	substrate					1539:1547	a good functional substrate	1521:1547	it a good functional substrate which could be applied in many fields	1518:1585	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	7	9	theme	functional	1528:1537	arg1	substrate					1539:1547	a good functional substrate	1521:1547	it a good functional substrate which could be applied in many fields	1518:1585	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	7	10	theme	alginate	1494:1501	arg1	fabric					1503:1508	alginate fabric	1494:1508	alginate fabric	1494:1508	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	1	11	theme	dodecyl	342:348	arg1	SDS					359:361	SDS	359:361	SDS	359:361	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	1	11	theme	dodecyl	342:348	arg1	sulfate					350:356	sodium dodecyl sulfate	335:356	sodium dodecyl sulfate (SDS)	335:362	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	5	12	theme	electrical	1181:1190	arg1	conductivity					1192:1203	the electrical conductivity	1177:1203	the electrical conductivity of alginate fabric	1177:1222	It was found that the electrical conductivity of alginate fabric could be intensively increased after PPy/Ag coating.					
34364607	0	13	theme	UV	97:98	arg1	resistance					100:109	UV resistance	97:109	UV resistance	97:109	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	0	13	theme	UV	97:98	arg1	fabrics					65:71	alginate fabrics	56:71	alginate fabrics with high conductivity	56:94	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	3	14	theme	reaction	621:628	arg1	time					630:633	the reaction time	617:633	the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively	617:955	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	15	theme	fabrics	1031:1037	arg1	performance					1007:1017	the performance	1003:1017	the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability	1003:1140	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	1	16	theme	pyrrole	278:284	arg1	monomer					291:297	pyrrole (Py) monomer	278:297	pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant	278:376	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	6	17	theme	anti-ultraviolet	1291:1306	arg1	capability					1308:1317	the anti-ultraviolet capability	1287:1317	the anti-ultraviolet capability	1287:1317	Meantime, the anti-ultraviolet capability and hydrophobicity could be largely improved by PPy/Ag coating especially under high Py dosage.					
34364607	3	18	theme	thermogravimetry	911:926	arg1	analysis					928:935	the thermogravimetry analysis	907:935	the thermogravimetry analysis	907:935	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	18	theme	thermogravimetry	911:926	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	19	theme	coating	992:998	arg1	influences					971:980	the influences	967:980	the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability	967:1140	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	1	20	theme	PPy/Ag	173:178	arg1	composite					181:189	the polypyrrole/silver (PPy/Ag) composite	149:189	the polypyrrole/silver (PPy/Ag) composite	149:189	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	2	21	theme	silver	414:419	arg1	nitrate					421:427	silver nitrate	414:427	silver nitrate	414:427	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	2	22	theme	Py	408:409	arg1	ratio					399:403	mole ratio	394:403	mole ratio of Py to silver nitrate	394:427	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	2	22	theme	Py	408:409	arg1	concentration					455:467	dopant concentration	448:467	dopant concentration	448:467	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	2	22	theme	Py	408:409	arg1	time					439:442	reaction time	430:442	reaction time	430:442	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	3	23	theme	diffraction	812:822	arg1	XRD					838:840	XRD	838:840	XRD	838:840	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	23	theme	diffraction	812:822	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	23	theme	diffraction	812:822	arg1	spectroscopy					824:835	X-ray diffraction spectroscopy	806:835	X-ray diffraction spectroscopy (XRD)	806:841	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	23	theme	diffraction	812:822	arg1	TGA					938:940	TGA	938:940	TGA	938:940	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	6	24	theme	Py	1404:1405	arg1	dosage					1407:1412	high Py dosage	1399:1412	high Py dosage	1399:1412	Meantime, the anti-ultraviolet capability and hydrophobicity could be largely improved by PPy/Ag coating especially under high Py dosage.					
34364607	2	25	theme	concentration	455:467	arg1	effects					383:389	The effects	379:389	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite	379:506	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	0	26	theme	In-situ	0:6	arg1	synthesis					8:16	In-situ synthesis	0:16	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.	0:129	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	3	27	theme	X-ray	844:848	arg1	XPS					878:880	XPS	878:880	XPS	878:880	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	27	theme	X-ray	844:848	arg1	spectroscopy					864:875	X-ray photoelectron spectroscopy	844:875	X-ray photoelectron spectroscopy (XPS)	844:881	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	27	theme	X-ray	844:848	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	0	28	theme	polypyrrole/silver	21:38	arg1	synthesis					8:16	In-situ synthesis	0:16	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.	0:129	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	1	29	theme	Py	287:288	arg1	monomer					291:297	pyrrole (Py) monomer	278:297	pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant	278:376	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	3	30	theme	photoelectron	850:862	arg1	XPS					878:880	XPS	878:880	XPS	878:880	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	30	theme	photoelectron	850:862	arg1	spectroscopy					864:875	X-ray photoelectron spectroscopy	844:875	X-ray photoelectron spectroscopy (XPS)	844:881	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	30	theme	photoelectron	850:862	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	2	31	theme	mole	394:397	arg1	ratio					399:403	mole ratio	394:403	mole ratio of Py to silver nitrate	394:427	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	2	32	theme	ratio	399:403	arg1	effects					383:389	The effects	379:389	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite	379:506	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	7	33	theme	many	1575:1578	arg1	fields					1580:1585	many fields	1575:1585	many fields	1575:1585	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	6	34	theme	PPy/Ag	1367:1372	arg1	coating					1374:1380	PPy/Ag coating	1367:1380	PPy/Ag coating	1367:1380	Meantime, the anti-ultraviolet capability and hydrophobicity could be largely improved by PPy/Ag coating especially under high Py dosage.					
34364607	5	35	theme	PPy/Ag	1261:1266	arg1	coating					1268:1274	PPy/Ag coating	1261:1274	PPy/Ag coating	1261:1274	It was found that the electrical conductivity of alginate fabric could be intensively increased after PPy/Ag coating.					
34364607	2	36	theme	reaction	430:437	arg1	time					439:442	reaction time	430:442	reaction time	430:442	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	1	37	theme	monomer	291:297	arg1	polymerization					260:273	chemical oxidative polymerization	241:273	chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant	241:376	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	2	38	theme	composite	498:506	arg1	preparation					476:486	the preparation	472:486	the preparation of PPy/Ag composite	472:506	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	3	39	theme	molar	549:553	arg1	1:1.5					589:593	1:1.5	589:593	1:1.5	589:593	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	39	theme	molar	549:553	arg1	ratio					555:559	the optimal molar ratio	537:559	the optimal molar ratio of Py to silver nitrate	537:583	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	40	theme	PPy/Ag	985:990	arg1	coating					992:998	PPy/Ag coating	985:998	PPy/Ag coating	985:998	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	3	41	dep	time	630:633	arg1	analyzed					719:726	analyzed	719:726	were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively	714:955	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	42	theme	alginate	1022:1029	arg1	hydrophilicity					1074:1087	hydrophilicity	1074:1087	hydrophilicity	1074:1087	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	4	42	theme	alginate	1022:1029	arg1	fabrics					1031:1037	alginate fabrics	1022:1037	alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability	1022:1140	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	4	42	theme	alginate	1022:1029	arg1	conductivity					1060:1071	electrical conductivity	1049:1071	electrical conductivity	1049:1071	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	4	42	theme	alginate	1022:1029	arg1	capability					1131:1140	anti-ultraviolet capability	1114:1140	anti-ultraviolet capability	1114:1140	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	4	42	theme	alginate	1022:1029	arg1	property					1101:1108	antistatic property	1090:1108	antistatic property	1090:1108	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	2	43	theme	PPy/Ag	491:496	arg1	composite					498:506	PPy/Ag composite	491:506	PPy/Ag composite	491:506	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	7	44	theme	direct	1484:1489	arg1	PPy/Ag					1467:1472	PPy/Ag composite direct	1467:1489	PPy/Ag composite direct on alginate fabric	1467:1508	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	3	45	theme	composite	704:712	arg1	properties					673:682	properties	673:682	properties	673:682	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	45	theme	composite	704:712	arg1	microstructure					654:667	the microstructure	650:667	the microstructure	650:667	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	1	46	theme	silver	305:310	arg1	dopant					371:376	the dopant	367:376	the dopant	367:376	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	1	46	theme	silver	305:310	arg1	nitrate					312:318	silver nitrate	305:318	silver nitrate	305:318	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	3	47	theme	resultant	687:695	arg1	composite					704:712	resultant PPy/Ag composite	687:712	resultant PPy/Ag composite	687:712	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	48	theme	antistatic	1090:1099	arg1	property					1101:1108	antistatic property	1090:1108	antistatic property	1090:1108	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	6	49	theme	high	1399:1402	arg1	dosage					1407:1412	high Py dosage	1399:1412	high Py dosage	1399:1412	Meantime, the anti-ultraviolet capability and hydrophobicity could be largely improved by PPy/Ag coating especially under high Py dosage.					
34364607	0	50	theme	alginate	56:63	arg1	resistance					100:109	UV resistance	97:109	UV resistance	97:109	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	0	50	theme	alginate	56:63	arg1	hydrophobicity					115:128	hydrophobicity	115:128	hydrophobicity	115:128	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	0	50	theme	alginate	56:63	arg1	fabrics					65:71	alginate fabrics	56:71	alginate fabrics with high conductivity	56:94	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	3	51	theme	PPy/Ag	697:702	arg1	composite					704:712	resultant PPy/Ag composite	687:712	resultant PPy/Ag composite	687:712	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	52	theme	optimal	541:547	arg1	1:1.5					589:593	1:1.5	589:593	1:1.5	589:593	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	52	theme	optimal	541:547	arg1	ratio					555:559	the optimal molar ratio	537:559	the optimal molar ratio of Py to silver nitrate	537:583	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	53	theme	anti-ultraviolet	1114:1129	arg1	capability					1131:1140	anti-ultraviolet capability	1114:1140	anti-ultraviolet capability	1114:1140	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	5	54	theme	alginate	1208:1215	arg1	fabric					1217:1222	alginate fabric	1208:1222	alginate fabric	1208:1222	It was found that the electrical conductivity of alginate fabric could be intensively increased after PPy/Ag coating.					
34364607	3	55	theme	Py	564:565	arg1	1:1.5					589:593	1:1.5	589:593	1:1.5	589:593	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	55	theme	Py	564:565	arg1	ratio					555:559	the optimal molar ratio	537:559	the optimal molar ratio of Py to silver nitrate	537:583	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	2	56	from	effects	383:389	arg1	preparation					476:486	the preparation	472:486	the preparation of PPy/Ag composite	472:506	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	7	57	theme	composite	1474:1482	arg1	PPy/Ag					1467:1472	PPy/Ag composite direct	1467:1489	PPy/Ag composite direct on alginate fabric	1467:1508	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	5	58	theme	fabric	1217:1222	arg1	conductivity					1192:1203	the electrical conductivity	1177:1203	the electrical conductivity of alginate fabric	1177:1222	It was found that the electrical conductivity of alginate fabric could be intensively increased after PPy/Ag coating.					
34364607	1	59	theme	alginate	221:228	arg1	fabrics					230:236	alginate fabrics	221:236	alginate fabrics	221:236	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	3	60	theme	0.02 M	600:605	arg1	SDS					607:609	0.02 M SDS	600:609	0.02 M SDS	600:609	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	61	theme	silver	570:575	arg1	nitrate					577:583	silver nitrate	570:583	silver nitrate	570:583	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	0	62	with	fabrics	65:71	arg1	conductivity					83:94	high conductivity	78:94	high conductivity	78:94	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	4	63	from	influences	971:980	arg1	performance					1007:1017	the performance	1003:1017	the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability	1003:1140	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	3	64	theme	Fourier	767:773	arg1	spectrometer					784:795	Fourier infrared spectrometer	767:795	Fourier infrared spectrometer (FT-IR)	767:803	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	64	theme	Fourier	767:773	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	64	theme	Fourier	767:773	arg1	FT-IR					798:802	FT-IR	798:802	FT-IR	798:802	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	4	65	theme	electrical	1049:1058	arg1	conductivity					1060:1071	electrical conductivity	1049:1071	electrical conductivity	1049:1071	Finally, the influences of PPy/Ag coating on the performance of alginate fabrics including electrical conductivity, hydrophilicity, antistatic property and anti-ultraviolet capability were determined.					
34364607	0	66	theme	high	78:81	arg1	conductivity					83:94	high conductivity	78:94	high conductivity	78:94	In-situ synthesis of polypyrrole/silver for fabricating alginate fabrics with high conductivity, UV resistance and hydrophobicity.					
34364607	1	67	dep	first	195:199	arg1	in-situ					201:207	in-situ	201:207	in-situ	201:207	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
34364607	3	68	theme	infrared	775:782	arg1	spectrometer					784:795	Fourier infrared spectrometer	767:795	Fourier infrared spectrometer (FT-IR)	767:803	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	68	theme	infrared	775:782	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	68	theme	infrared	775:782	arg1	FT-IR					798:802	FT-IR	798:802	FT-IR	798:802	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	7	69	theme	good	1523:1526	arg1	substrate					1539:1547	a good functional substrate	1521:1547	it a good functional substrate which could be applied in many fields	1518:1585	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	2	70	theme	dopant	448:453	arg1	concentration					455:467	dopant concentration	448:467	dopant concentration	448:467	The effects of mole ratio of Py to silver nitrate, reaction time and dopant concentration on the preparation of PPy/Ag composite were optimized.					
34364607	7	71	theme	simple	1439:1444	arg1	method					1446:1451	a simple method	1437:1451	a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields	1437:1585	This paper introduced a simple method for preparing PPy/Ag composite direct on alginate fabric to make it a good functional substrate which could be applied in many fields.					
34364607	3	72	theme	scanning	731:738	arg1	analysis					928:935	the thermogravimetry analysis	907:935	the thermogravimetry analysis	907:935	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	72	theme	scanning	731:738	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	72	theme	scanning	731:738	arg1	spectrometer					784:795	Fourier infrared spectrometer	767:795	Fourier infrared spectrometer (FT-IR)	767:803	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	72	theme	scanning	731:738	arg1	spectroscopy					890:901	Raman spectroscopy	884:901	Raman spectroscopy	884:901	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	72	theme	scanning	731:738	arg1	spectroscopy					824:835	X-ray diffraction spectroscopy	806:835	X-ray diffraction spectroscopy (XRD)	806:841	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	72	theme	scanning	731:738	arg1	SEM					761:763	SEM	761:763	SEM	761:763	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	72	theme	scanning	731:738	arg1	spectroscopy					864:875	X-ray photoelectron spectroscopy	844:875	X-ray photoelectron spectroscopy (XPS)	844:881	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	analysis					928:935	the thermogravimetry analysis	907:935	the thermogravimetry analysis	907:935	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	microscope					749:758	scanning electron microscope	731:758	scanning electron microscope (SEM)	731:764	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	spectrometer					784:795	Fourier infrared spectrometer	767:795	Fourier infrared spectrometer (FT-IR)	767:803	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	spectroscopy					890:901	Raman spectroscopy	884:901	Raman spectroscopy	884:901	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	spectroscopy					824:835	X-ray diffraction spectroscopy	806:835	X-ray diffraction spectroscopy (XRD)	806:841	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	SEM					761:763	SEM	761:763	SEM	761:763	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	3	73	theme	electron	740:747	arg1	spectroscopy					864:875	X-ray photoelectron spectroscopy	844:875	X-ray photoelectron spectroscopy (XPS)	844:881	It was found the optimal molar ratio of Py to silver nitrate was 1:1.5 with 0.02 M SDS under the reaction time of 10 h. Then, the microstructure and properties of resultant PPy/Ag composite were analyzed by scanning electron microscope (SEM), Fourier infrared spectrometer (FT-IR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and the thermogravimetry analysis (TGA), respectively.					
34364607	1	74	theme	chemical	241:248	arg1	polymerization					260:273	chemical oxidative polymerization	241:273	chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant	241:376	In this research, the polypyrrole/silver (PPy/Ag) composite was first in-situ prepared on alginate fabrics by chemical oxidative polymerization of pyrrole (Py) monomer using silver nitrate as oxidant and sodium dodecyl sulfate (SDS) as the dopant.					
32307161	7	0	theme	slight	961:966	arg1	recovery					968:975	a slight recovery	959:975	a slight recovery	959:975	Urea content decreased during the study, showing a slight recovery at 4 wk.					
32307161	12	1	theme	mature	1551:1556	arg1	milk					1558:1561	mature milk	1551:1561	mature milk	1551:1561	In the case of deer, milk should be considered transitional from 24 to 48 h after parturition, and samples collected after 2 wk can be considered mature milk.					
32307161	9	2	theme	h	1146:1146	arg1	postpartum					1148:1157	5 h postpartum	1144:1157	5 h postpartum	1144:1157	The IgG content was higher before 5 h postpartum than at 24 h postpartum.					
32307161	6	3	theme	dry	820:822	arg1	matter					824:829	dry matter	820:829	dry matter	820:829	Similarly, dry matter dropped during the first 24 h and then remained constant throughout the study.					
32307161	2	4	theme	first	416:420	arg1	day					422:424	the first day	412:424	the first day after parturition	412:442	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	2	5	theme	48	473:474	arg1	h					476:476	h	476:476	h	476:476	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	1	6	theme	somatic	132:138	arg1	count					145:149	somatic cell count	132:149	somatic cell count	132:149	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	10	7	theme	lactation	1275:1283	arg1	wk					1269:1270	4 wk	1267:1270	4 wk of lactation	1267:1283	After 5 h, the level of IgG decreased progressively until it reached 0.18 mg/mL at 4 wk of lactation.					
32307161	1	8	dep	deer	206:209	arg1	elaphus					219:225	Cervus elaphus	212:225	Cervus elaphus	212:225	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	5	9	from	beginning	748:756	arg1	low					737:739	low	737:739	low	737:739	Conversely, lactose concentration was low in the beginning (<5 h), increasing gradually throughout the study.					
32307161	3	10	theme	study	526:530	arg1	period					532:537	the study period	522:537	the study period	522:537	Fat content did not vary during the study period.					
32307161	4	11	theme	protein	555:561	arg1	contents					574:581	total protein and casein contents	549:581	total protein and casein contents	549:581	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	1	12	theme	different	271:279	arg1	times					281:285	different times	271:285	different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk)	271:338	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	2	13	theme	intermediate	450:461	arg1	values					463:468	intermediate values	450:468	intermediate values at 48 h postpartum	450:487	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	1	14	theme	cell	140:143	arg1	count					145:149	somatic cell count	132:149	somatic cell count	132:149	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	2	15	theme	production	345:354	arg1	level					356:360	The production level	341:360	The production level	341:360	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	2	15	theme	production	345:354	arg1	higher					366:371	higher	366:371	higher	366:371	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	1	16	from	changes	99:105	arg1	composition					119:129	chemical composition	110:129	chemical composition	110:129	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	16	from	changes	99:105	arg1	IgG					174:176	IgG	174:176	IgG	174:176	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	16	from	changes	99:105	arg1	colostrum					228:236	red deer (Cervus elaphus) colostrum	202:236	red deer (Cervus elaphus) colostrum	202:236	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	16	from	changes	99:105	arg1	count					145:149	somatic cell count	132:149	somatic cell count	132:149	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	16	from	changes	99:105	arg1	G					171:171	immunoglobulin G	156:171	immunoglobulin G (IgG)	156:177	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	4	17	theme	casein	567:572	arg1	contents					574:581	total protein and casein contents	549:581	total protein and casein contents	549:581	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	9	18	theme	h	1170:1170	arg1	postpartum					1172:1181	24 h postpartum	1167:1181	24 h postpartum	1167:1181	The IgG content was higher before 5 h postpartum than at 24 h postpartum.					
32307161	8	19	theme	cell	994:997	arg1	count					999:1003	Somatic cell count	986:1003	Somatic cell count	986:1003	Somatic cell count was higher during the first hours after parturition and gradually decreased throughout the study period.					
32307161	0	20	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Red deer (Cervus elaphus)	0:45	Short communication: Red deer (Cervus elaphus) colostrum during its transition to milk.					
32307161	1	21	theme	red	202:204	arg1	deer					206:209	red deer	202:209	red deer (Cervus elaphus) colostrum	202:236	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	12	22	dep	48	1476:1477	arg1	to					1473:1474	to	1473:1474	to	1473:1474	In the case of deer, milk should be considered transitional from 24 to 48 h after parturition, and samples collected after 2 wk can be considered mature milk.					
32307161	8	23	theme	Somatic	986:992	arg1	count					999:1003	Somatic cell count	986:1003	Somatic cell count	986:1003	Somatic cell count was higher during the first hours after parturition and gradually decreased throughout the study period.					
32307161	0	24	theme	Red	21:23	arg1	deer					25:28	Red deer	21:28	Short communication: Red deer (Cervus elaphus)	0:45	Short communication: Red deer (Cervus elaphus) colostrum during its transition to milk.					
32307161	1	25	theme	deer	206:209	arg1	colostrum					228:236	red deer (Cervus elaphus) colostrum	202:236	red deer (Cervus elaphus) colostrum	202:236	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	26	from	content	191:197	arg1	composition					119:129	chemical composition	110:129	chemical composition	110:129	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	26	from	content	191:197	arg1	IgG					174:176	IgG	174:176	IgG	174:176	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	26	from	content	191:197	arg1	colostrum					228:236	red deer (Cervus elaphus) colostrum	202:236	red deer (Cervus elaphus) colostrum	202:236	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	26	from	content	191:197	arg1	count					145:149	somatic cell count	132:149	somatic cell count	132:149	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	26	from	content	191:197	arg1	G					171:171	immunoglobulin G	156:171	immunoglobulin G (IgG)	156:177	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	2	27	theme	h	476:476	arg1	postpartum					478:487	48 h postpartum	473:487	48 h postpartum	473:487	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	4	28	theme	total	549:553	arg1	contents					574:581	total protein and casein contents	549:581	total protein and casein contents	549:581	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	12	29	theme	deer	1420:1423	arg1	case					1412:1415	the case	1408:1415	the case of deer	1408:1423	In the case of deer, milk should be considered transitional from 24 to 48 h after parturition, and samples collected after 2 wk can be considered mature milk.					
32307161	1	30	theme	immunoglobulin	156:169	arg1	G					171:171	immunoglobulin G	156:171	immunoglobulin G (IgG)	156:177	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	30	theme	immunoglobulin	156:169	arg1	IgG					174:176	IgG	174:176	IgG	174:176	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	11	31	theme	IgM	1320:1322	arg1	content					1324:1330	IgM content	1320:1330	IgM content	1320:1330	We observed a similar pattern for IgM content, but it decreased more quickly than IgG and was not detected after 2 wk.					
32307161	6	32	theme	first	850:854	arg1	h					859:859	the first 24 h	846:859	the first 24 h	846:859	Similarly, dry matter dropped during the first 24 h and then remained constant throughout the study.					
32307161	2	33	from	postpartum	478:487	arg1	values					463:468	intermediate values	450:468	intermediate values at 48 h postpartum	450:487	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	4	34	theme	initial	613:619	arg1	h					623:623	the initial 5 h	609:623	the initial 5 h after parturition	609:641	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	7	35	theme	Urea	910:913	arg1	content					915:921	Urea content	910:921	Urea content	910:921	Urea content decreased during the study, showing a slight recovery at 4 wk.					
32307161	1	36	theme	M	183:183	arg1	content					191:197	M (IgM) content	183:197	M (IgM) content in red deer (Cervus elaphus) colostrum	183:236	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	4	37	from	h	623:623	arg1	high					601:604	high	601:604	high	601:604	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	10	38	theme	IgG	1208:1210	arg1	level					1199:1203	the level	1195:1203	the level of IgG	1195:1210	After 5 h, the level of IgG decreased progressively until it reached 0.18 mg/mL at 4 wk of lactation.					
32307161	9	39	theme	24	1167:1168	arg1	h					1170:1170	h	1170:1170	h	1170:1170	The IgG content was higher before 5 h postpartum than at 24 h postpartum.					
32307161	8	40	theme	first	1027:1031	arg1	hours					1033:1037	the first hours	1023:1037	the first hours after parturition	1023:1055	Somatic cell count was higher during the first hours after parturition and gradually decreased throughout the study period.					
32307161	9	41	theme	IgG	1114:1116	arg1	higher					1130:1135	higher	1130:1135	higher	1130:1135	The IgG content was higher before 5 h postpartum than at 24 h postpartum.					
32307161	9	41	theme	IgG	1114:1116	arg1	content					1118:1124	The IgG content	1110:1124	The IgG content	1110:1124	The IgG content was higher before 5 h postpartum than at 24 h postpartum.					
32307161	2	42	theme	lactation	390:398	arg1	wk					384:385	2 and 4 wk	376:385	2 and 4 wk of lactation	376:398	The production level was higher at 2 and 4 wk of lactation than during the first day after parturition, with intermediate values at 48 h postpartum.					
32307161	0	43	dep	deer	25:28	arg1	elaphus					38:44	Cervus elaphus	31:44	Cervus elaphus	31:44	Short communication: Red deer (Cervus elaphus) colostrum during its transition to milk.					
32307161	5	44	from	low	737:739	arg1	beginning					748:756	the beginning	744:756	the beginning (<5 h)	744:763	Conversely, lactose concentration was low in the beginning (<5 h), increasing gradually throughout the study.					
32307161	5	44	from	low	737:739	arg1	h					762:762	<5 h	759:762	<5 h	759:762	Conversely, lactose concentration was low in the beginning (<5 h), increasing gradually throughout the study.					
32307161	12	45	from	h	1479:1479	arg1	transitional					1452:1463	transitional	1452:1463	transitional	1452:1463	In the case of deer, milk should be considered transitional from 24 to 48 h after parturition, and samples collected after 2 wk can be considered mature milk.					
32307161	4	46	theme	postpartum	687:696	arg1	h					685:685	24 h postpartum	682:696	24 h postpartum	682:696	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	3	47	theme	Fat	490:492	arg1	content					494:500	Fat content	490:500	Fat content	490:500	Fat content did not vary during the study period.					
32307161	8	48	theme	study	1096:1100	arg1	period					1102:1107	the study period	1092:1107	the study period	1092:1107	Somatic cell count was higher during the first hours after parturition and gradually decreased throughout the study period.					
32307161	5	49	theme	lactose	711:717	arg1	concentration					719:731	lactose concentration	711:731	lactose concentration	711:731	Conversely, lactose concentration was low in the beginning (<5 h), increasing gradually throughout the study.					
32307161	1	50	dep	h	309:309	arg1	wk					336:337	4 wk	334:337	4 wk	334:337	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	50	dep	h	309:309	arg1	h					315:315	24 h	312:315	24 h	312:315	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	50	dep	h	309:309	arg1	h					321:321	48 h	318:321	48 h	318:321	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	50	dep	h	309:309	arg1	wk					326:327	2 wk	324:327	2 wk	324:327	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	0	51	dep	communication	6:18	arg1	deer					25:28	Red deer	21:28	Short communication: Red deer (Cervus elaphus)	0:45	Short communication: Red deer (Cervus elaphus) colostrum during its transition to milk.					
32307161	4	52	from	high	601:604	arg1	h					623:623	the initial 5 h	609:623	the initial 5 h after parturition	609:641	However, total protein and casein contents were particularly high in the initial 5 h after parturition, decreasing to approximately 50% after 24 h postpartum.					
32307161	1	53	theme	chemical	110:117	arg1	composition					119:129	chemical composition	110:129	chemical composition	110:129	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	1	54	dep	parturition	293:303	arg1	h					309:309	<5 h	306:309	<5 h	306:309	We studied changes in chemical composition, somatic cell count, and immunoglobulin G (IgG) and M (IgM) content in red deer (Cervus elaphus) colostrum during the transition to milk at different times after parturition (<5 h, 24 h, 48 h, 2 wk, and 4 wk).					
32307161	11	55	theme	similar	1300:1306	arg1	pattern					1308:1314	a similar pattern	1298:1314	a similar pattern for IgM content	1298:1330	We observed a similar pattern for IgM content, but it decreased more quickly than IgG and was not detected after 2 wk.					
33091995	8	0	theme	cheese	1030:1035	arg1	better					1078:1083	better	1078:1083	better	1078:1083	Compared with the initial properties of cheese, the characteristics of cheese packaged with CS-PC films were obviously better than those of the control groups.					
33091995	8	0	theme	cheese	1030:1035	arg1	characteristics					1011:1025	the characteristics	1007:1025	the characteristics of cheese packaged with CS-PC films	1007:1061	Compared with the initial properties of cheese, the characteristics of cheese packaged with CS-PC films were obviously better than those of the control groups.					
33091995	0	1	with	films	35:39	arg1	activity					63:70	high antioxidant activity	46:70	high antioxidant activity	46:70	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	0	1	with	films	35:39	arg1	responsivity					79:90	pH responsivity	76:90	pH responsivity	76:90	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	8	2	theme	control	1103:1109	arg1	groups					1111:1116	the control groups	1099:1116	the control groups	1099:1116	Compared with the initial properties of cheese, the characteristics of cheese packaged with CS-PC films were obviously better than those of the control groups.					
33091995	0	3	theme	pH	76:77	arg1	responsivity					79:90	pH responsivity	76:90	pH responsivity	76:90	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	5	4	theme	antioxidant	609:619	arg1	activity					621:628	good antioxidant activity	604:628	good antioxidant activity	604:628	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	0	5	theme	cheese	96:101	arg1	packaging					103:111	cheese packaging	96:111	cheese packaging	96:111	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	3	6	theme	CS-control	373:382	arg1	film					384:387	CS-control film	373:387	CS-control film	373:387	Compared with CS-control film, CS-PC films exhibited lower solubility and higher tensile strength.					
33091995	2	7	theme	antioxidant	275:285	arg1	properties					287:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	7	theme	antioxidant	275:285	arg1	activity					313:320	antibacterial activity	299:320	antibacterial activity	299:320	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	7	theme	antioxidant	275:285	arg1	responsivity					329:340	pH responsivity	326:340	pH responsivity	326:340	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	6	8	theme	pH	728:729	arg1	responsivity					731:742	pH responsivity	728:742	pH responsivity	728:742	pH responsivity was represented by the outstanding changes in color, which were visible to the naked eye.					
33091995	6	9	from	changes	779:785	arg1	color					790:794	color	790:794	color	790:794	pH responsivity was represented by the outstanding changes in color, which were visible to the naked eye.					
33091995	5	10	dep	times	693:697	arg1	higher					699:704	higher	699:704	higher	699:704	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	1	11	with	films	145:149	arg1	ratios					185:190	different mass ratios	170:190	different mass ratios	170:190	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	7	12	theme	antioxidant	864:874	arg1	activity					876:883	high antioxidant activity	859:883	high antioxidant activity	859:883	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	1	13	theme	different	170:178	arg1	ratios					185:190	different mass ratios	170:190	different mass ratios	170:190	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	3	14	theme	lower	412:416	arg1	solubility					418:427	lower solubility	412:427	lower solubility	412:427	Compared with CS-control film, CS-PC films exhibited lower solubility and higher tensile strength.					
33091995	8	15	theme	CS-PC	1051:1055	arg1	films					1057:1061	CS-PC films	1051:1061	CS-PC films	1051:1061	Compared with the initial properties of cheese, the characteristics of cheese packaged with CS-PC films were obviously better than those of the control groups.					
33091995	5	16	theme	CS-PC	582:586	arg1	films					588:592	CS-PC films	582:592	CS-PC films	582:592	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	1	17	theme	mass	180:183	arg1	ratios					185:190	different mass ratios	170:190	different mass ratios	170:190	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	3	18	theme	CS-PC	390:394	arg1	films					396:400	CS-PC films	390:400	CS-PC films	390:400	Compared with CS-control film, CS-PC films exhibited lower solubility and higher tensile strength.					
33091995	5	19	theme	ABTS+	653:657	arg1	rates					670:674	ABTS+ scavenging rates	653:674	ABTS+ scavenging rates	653:674	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	8	20	theme	cheese	999:1004	arg1	properties					985:994	the initial properties	973:994	the initial properties of cheese	973:1004	Compared with the initial properties of cheese, the characteristics of cheese packaged with CS-PC films were obviously better than those of the control groups.					
33091995	3	21	theme	higher	433:438	arg1	strength					448:455	higher tensile strength	433:455	higher tensile strength	433:455	Compared with CS-control film, CS-PC films exhibited lower solubility and higher tensile strength.					
33091995	0	22	theme	Chitosan	0:7	arg1	films					35:39	Chitosan and procyanidin composite films	0:39	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.	0:112	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	5	23	theme	scavenging	659:668	arg1	rates					670:674	ABTS+ scavenging rates	653:674	ABTS+ scavenging rates	653:674	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	7	24	theme	Food	834:837	arg1	film					849:852	Food packaging film	834:852	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity	834:930	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	3	25	theme	tensile	440:446	arg1	strength					448:455	higher tensile strength	433:455	higher tensile strength	433:455	Compared with CS-control film, CS-PC films exhibited lower solubility and higher tensile strength.					
33091995	0	26	theme	composite	25:33	arg1	films					35:39	Chitosan and procyanidin composite films	0:39	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.	0:112	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	5	27	theme	good	604:607	arg1	activity					621:628	good antioxidant activity	604:628	good antioxidant activity	604:628	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	2	28	theme	antibacterial	299:311	arg1	properties					287:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	28	theme	antibacterial	299:311	arg1	activity					313:320	antibacterial activity	299:320	antibacterial activity	299:320	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	6	29	theme	naked	823:827	arg1	eye					829:831	the naked eye	819:831	the naked eye	819:831	pH responsivity was represented by the outstanding changes in color, which were visible to the naked eye.					
33091995	0	30	theme	procyanidin	13:23	arg1	films					35:39	Chitosan and procyanidin composite films	0:39	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.	0:112	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	4	31	theme	antimicrobial	462:474	arg1	properties					476:485	The antimicrobial properties	458:485	The antimicrobial properties against Escherichia coli and Aspergillus niger	458:532	The antimicrobial properties against Escherichia coli and Aspergillus niger were improved by 20.0% and 30.4%, respectively.					
33091995	8	32	theme	initial	977:983	arg1	properties					985:994	the initial properties	973:994	the initial properties of cheese	973:1004	Compared with the initial properties of cheese, the characteristics of cheese packaged with CS-PC films were obviously better than those of the control groups.					
33091995	2	33	theme	structural	240:249	arg1	properties					287:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	33	theme	structural	240:249	arg1	activity					313:320	antibacterial activity	299:320	antibacterial activity	299:320	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	33	theme	structural	240:249	arg1	responsivity					329:340	pH responsivity	326:340	pH responsivity	326:340	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	1	34	theme	solution	209:216	arg1	method					226:231	solution casting method	209:231	solution casting method	209:231	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	7	35	theme	high	859:862	arg1	activity					876:883	high antioxidant activity	859:883	high antioxidant activity	859:883	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	1	36	theme	casting	218:224	arg1	method					226:231	solution casting method	209:231	solution casting method	209:231	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	0	37	theme	antioxidant	51:61	arg1	activity					63:70	high antioxidant activity	46:70	high antioxidant activity	46:70	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	7	38	theme	packaging	839:847	arg1	film					849:852	Food packaging film	834:852	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity	834:930	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	5	39	theme	2.45	688:691	arg1	times					693:697	times	693:697	times	693:697	CS-PC films indicated good antioxidant activity through their DPPH and ABTS+ scavenging rates, which were 2.45 times higher than CS-control film.					
33091995	1	40	theme	Chitosan-procyanidin	114:133	arg1	films					145:149	Chitosan-procyanidin composite films	114:149	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios	114:190	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	1	40	theme	Chitosan-procyanidin	114:133	arg1	films					158:162	CS-PC films	152:162	CS-PC films	152:162	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	0	41	theme	high	46:49	arg1	activity					63:70	high antioxidant activity	46:70	high antioxidant activity	46:70	Chitosan and procyanidin composite films with high antioxidant activity and pH responsivity for cheese packaging.					
33091995	7	42	with	film	849:852	arg1	activity					876:883	high antioxidant activity	859:883	high antioxidant activity	859:883	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	7	42	with	film	849:852	arg1	properties					901:910	bacteriostatic properties	886:910	bacteriostatic properties	886:910	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	7	42	with	film	849:852	arg1	responsivity					919:930	pH responsivity	916:930	pH responsivity	916:930	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	6	43	theme	outstanding	767:777	arg1	changes					779:785	the outstanding changes	763:785	the outstanding changes in color, which were visible to the naked eye	763:831	pH responsivity was represented by the outstanding changes in color, which were visible to the naked eye.					
33091995	1	44	theme	composite	135:143	arg1	films					145:149	Chitosan-procyanidin composite films	114:149	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios	114:190	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	1	44	theme	composite	135:143	arg1	films					158:162	CS-PC films	152:162	CS-PC films	152:162	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	2	45	theme	physical	261:268	arg1	properties					287:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	45	theme	physical	261:268	arg1	activity					313:320	antibacterial activity	299:320	antibacterial activity	299:320	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	45	theme	physical	261:268	arg1	responsivity					329:340	pH responsivity	326:340	pH responsivity	326:340	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	46	theme	pH	326:327	arg1	properties					287:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	46	theme	pH	326:327	arg1	responsivity					329:340	pH responsivity	326:340	pH responsivity	326:340	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	47	theme	thermal	252:258	arg1	properties					287:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties	234:296	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	47	theme	thermal	252:258	arg1	activity					313:320	antibacterial activity	299:320	antibacterial activity	299:320	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	2	47	theme	thermal	252:258	arg1	responsivity					329:340	pH responsivity	326:340	pH responsivity	326:340	Their structural, thermal, physical, and antioxidant properties, antibacterial activity and pH responsivity were determined.					
33091995	1	48	theme	CS-PC	152:156	arg1	films					145:149	Chitosan-procyanidin composite films	114:149	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios	114:190	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	1	48	theme	CS-PC	152:156	arg1	films					158:162	CS-PC films	152:162	CS-PC films	152:162	Chitosan-procyanidin composite films (CS-PC films) with different mass ratios were prepared by solution casting method.					
33091995	7	49	theme	pH	916:917	arg1	responsivity					919:930	pH responsivity	916:930	pH responsivity	916:930	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33091995	7	50	theme	bacteriostatic	886:899	arg1	properties					901:910	bacteriostatic properties	886:910	bacteriostatic properties	886:910	Food packaging film with high antioxidant activity, bacteriostatic properties and pH responsivity was prepared by CS and PC.					
33245436	0	0	theme	thin	109:112	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	10	1	theme	sorbent	1436:1442	arg1	stability					1419:1427	The good stability	1410:1427	The good stability of the sorbent	1410:1442	The good stability of the sorbent enabled up to eight cycles of reuse.Graphical abstract.					
33245436	2	2	theme	polypyrrole	412:422	arg1	film					429:432	polypyrrole thin film	412:432	polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles	412:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	10	3	dep	eight	1458:1462	arg1	to					1455:1456	to	1455:1456	to	1455:1456	The good stability of the sorbent enabled up to eight cycles of reuse.Graphical abstract.					
33245436	0	4	theme	polypyrrole	97:107	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	9	5	theme	lower	1395:1399	arg1	range					1370:1374	the range	1366:1374	the range 84 to 99% and RSDs lower than 8%	1366:1407	This method exhibited good relative recoveries in the range 84 to 99% and RSDs lower than 8%.					
33245436	8	6	dep	range	1287:1291	arg1	to					1299:1300	to	1299:1300	to	1299:1300	Limits of detection were in the range 0.010 to 0.025 μg L-1.					
33245436	6	7	theme	developed	920:928	arg1	sorbent					930:936	The developed sorbent	916:936	The developed sorbent	916:936	The developed sorbent was coupled with GC-MS/MS to determine organophosphorus pesticides in fruit juices and vegetable.					
33245436	9	8	dep	%	1384:1384	arg1	to					1379:1380	to	1379:1380	to	1379:1380	This method exhibited good relative recoveries in the range 84 to 99% and RSDs lower than 8%.					
33245436	0	9	theme	porous	126:131	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	3	10	theme	calcium	635:641	arg1	carbonate					643:651	incorporated calcium carbonate	622:651	incorporated calcium carbonate	622:651	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	7	11	theme	linear	1098:1103	arg1	range					1105:1109	good linear range	1093:1109	good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1	1093:1190	Under optimized condition, the developed method provided good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1 for mevinphos, dimethoate, and parathion methyl, respectively.					
33245436	0	12	with	determination	175:187	arg1	GC-MS/MS					194:201	GC-MS/MS	194:201	GC-MS/MS	194:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	0	13	theme	film-coated	114:124	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	6	14	from	pesticides	994:1003	arg1	fruit					1008:1012	fruit juices	1008:1019	fruit juices	1008:1019	The developed sorbent was coupled with GC-MS/MS to determine organophosphorus pesticides in fruit juices and vegetable.					
33245436	6	14	from	pesticides	994:1003	arg1	vegetable					1025:1033	vegetable	1025:1033	vegetable	1025:1033	The developed sorbent was coupled with GC-MS/MS to determine organophosphorus pesticides in fruit juices and vegetable.					
33245436	1	15	theme	solvent-assisted	288:303	arg1	preconcentration					305:320	the solvent-assisted preconcentration	284:320	the solvent-assisted preconcentration of organophosphorus pesticides	284:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	0	16	theme	magnetic	143:150	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	3	17	theme	dioxide	585:591	arg1	bubbles					593:599	carbon dioxide bubbles	578:599	carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid	578:674	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	0	18	theme	composite	133:141	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	2	19	with	beads	475:479	arg1	nanoparticles					505:517	embedded magnetite nanoparticles	486:517	embedded magnetite nanoparticles	486:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	7	20	theme	parathion	1223:1231	arg1	methyl					1233:1238	parathion methyl	1223:1238	parathion methyl	1223:1238	Under optimized condition, the developed method provided good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1 for mevinphos, dimethoate, and parathion methyl, respectively.					
33245436	2	21	theme	magnetite	495:503	arg1	nanoparticles					505:517	embedded magnetite nanoparticles	486:517	embedded magnetite nanoparticles	486:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	1	22	theme	porous	206:211	arg1	phase					274:278	a solid phase	266:278	a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides	266:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	1	22	theme	porous	206:211	arg1	sorbent					232:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	0	23	theme	dispersive	17:26	arg1	extraction					47:56	Solvent-assisted dispersive liquid-solid phase extraction	0:56	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.	0:202	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	3	24	theme	carbon	578:583	arg1	bubbles					593:599	carbon dioxide bubbles	578:599	carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid	578:674	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	6	25	dep	fruit	1008:1012	arg1	juices					1014:1019	juices	1014:1019	juices	1014:1019	The developed sorbent was coupled with GC-MS/MS to determine organophosphorus pesticides in fruit juices and vegetable.					
33245436	7	26	theme	developed	1067:1075	arg1	method					1077:1082	the developed method	1063:1082	the developed method	1063:1082	Under optimized condition, the developed method provided good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1 for mevinphos, dimethoate, and parathion methyl, respectively.					
33245436	2	27	theme	porous	371:376	arg1	sorbent					388:394	The hierarchical porous composite sorbent	354:394	The hierarchical porous composite sorbent	354:394	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	3	28	with	reaction	610:617	arg1	acid					671:674	hydrochloric acid	658:674	hydrochloric acid	658:674	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	1	29	theme	composite	213:221	arg1	phase					274:278	a solid phase	266:278	a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides	266:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	1	29	theme	composite	213:221	arg1	sorbent					232:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	0	30	theme	Solvent-assisted	0:15	arg1	extraction					47:56	Solvent-assisted dispersive liquid-solid phase extraction	0:56	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.	0:202	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	10	31	theme	abstract	1490:1497	arg1	cycles					1464:1469	up to eight cycles	1452:1469	up to eight cycles of reuse.Graphical abstract	1452:1497	The good stability of the sorbent enabled up to eight cycles of reuse.Graphical abstract.					
33245436	9	32	theme	good	1338:1341	arg1	recoveries					1352:1361	good relative recoveries	1338:1361	good relative recoveries	1338:1361	This method exhibited good relative recoveries in the range 84 to 99% and RSDs lower than 8%.					
33245436	2	33	theme	hierarchical	358:369	arg1	sorbent					388:394	The hierarchical porous composite sorbent	354:394	The hierarchical porous composite sorbent	354:394	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	1	34	theme	magnetic	223:230	arg1	phase					274:278	a solid phase	266:278	a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides	266:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	1	34	theme	magnetic	223:230	arg1	sorbent					232:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	0	35	theme	phase	41:45	arg1	extraction					47:56	Solvent-assisted dispersive liquid-solid phase extraction	0:56	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.	0:202	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	0	36	theme	prior	160:164	arg1	sorbent					152:158	a polypyrrole thin film-coated porous composite magnetic sorbent	95:158	a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	95:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	9	37	theme	relative	1343:1350	arg1	recoveries					1352:1361	good relative recoveries	1338:1361	good relative recoveries	1338:1361	This method exhibited good relative recoveries in the range 84 to 99% and RSDs lower than 8%.					
33245436	7	38	theme	good	1093:1096	arg1	range					1105:1109	good linear range	1093:1109	good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1	1093:1190	Under optimized condition, the developed method provided good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1 for mevinphos, dimethoate, and parathion methyl, respectively.					
33245436	2	39	theme	beads	475:479	arg1	surface					448:454	the surface	444:454	the surface of porous alginate beads with embedded magnetite nanoparticles	444:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	0	40	theme	liquid-solid	28:39	arg1	extraction					47:56	Solvent-assisted dispersive liquid-solid phase extraction	0:56	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.	0:202	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	1	41	theme	organophosphorus	325:340	arg1	pesticides					342:351	organophosphorus pesticides	325:351	organophosphorus pesticides	325:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	6	42	theme	organophosphorus	977:992	arg1	pesticides					994:1003	organophosphorus pesticides	977:1003	organophosphorus pesticides in fruit juices and vegetable	977:1033	The developed sorbent was coupled with GC-MS/MS to determine organophosphorus pesticides in fruit juices and vegetable.					
33245436	2	43	theme	alginate	466:473	arg1	beads					475:479	porous alginate beads	459:479	porous alginate beads with embedded magnetite nanoparticles	459:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	1	44	theme	pesticides	342:351	arg1	preconcentration					305:320	the solvent-assisted preconcentration	284:320	the solvent-assisted preconcentration of organophosphorus pesticides	284:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	3	45	theme	hydrochloric	658:669	arg1	acid					671:674	hydrochloric acid	658:674	hydrochloric acid	658:674	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	2	46	theme	porous	459:464	arg1	beads					475:479	porous alginate beads	459:479	porous alginate beads with embedded magnetite nanoparticles	459:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	8	47	theme	detection	1265:1273	arg1	Limits					1255:1260	Limits	1255:1260	Limits of detection	1255:1273	Limits of detection were in the range 0.010 to 0.025 μg L-1.					
33245436	2	48	theme	embedded	486:493	arg1	nanoparticles					505:517	embedded magnetite nanoparticles	486:517	embedded magnetite nanoparticles	486:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	5	49	theme	extraction	836:845	arg1	conditions					847:856	sorbent fabrication and extraction conditions	812:856	conditions	847:856	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	0	50	theme	pesticides	78:87	arg1	extraction					47:56	Solvent-assisted dispersive liquid-solid phase extraction	0:56	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.	0:202	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	5	51	theme	sorbent	812:818	arg1	fabrication					820:830	sorbent fabrication and extraction conditions	812:856	fabrication	820:830	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	5	52	theme	extraction	892:901	arg1	performance					903:913	the best extraction performance	883:913	the best extraction performance	883:913	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	10	53	theme	reuse.Graphical	1474:1488	arg1	abstract					1490:1497	reuse.Graphical abstract	1474:1497	reuse.Graphical abstract	1474:1497	The good stability of the sorbent enabled up to eight cycles of reuse.Graphical abstract.					
33245436	3	54	theme	carbonate	643:651	arg1	reaction					610:617	the reaction	606:617	the reaction of incorporated calcium carbonate with hydrochloric acid	606:674	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	3	55	theme	hydrogel	546:553	arg1	beads					555:559	the alginate hydrogel beads	533:559	the alginate hydrogel beads	533:559	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	0	56	theme	organophosphorus	61:76	arg1	pesticides					78:87	organophosphorus pesticides	61:87	organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS	61:201	Solvent-assisted dispersive liquid-solid phase extraction of organophosphorus pesticides using a polypyrrole thin film-coated porous composite magnetic sorbent prior to their determination with GC-MS/MS.					
33245436	5	57	theme	fabricated	754:763	arg1	sorbent					782:788	The fabricated porous composite sorbent	750:788	The fabricated porous composite sorbent	750:788	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	3	58	from	pores	524:528	arg1	beads					555:559	the alginate hydrogel beads	533:559	the alginate hydrogel beads	533:559	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	3	59	from	reaction	610:617	arg1	bubbles					593:599	carbon dioxide bubbles	578:599	carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid	578:674	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	3	60	theme	incorporated	622:633	arg1	carbonate					643:651	incorporated calcium carbonate	622:651	incorporated calcium carbonate	622:651	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	5	61	theme	porous	765:770	arg1	sorbent					782:788	The fabricated porous composite sorbent	750:788	The fabricated porous composite sorbent	750:788	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	10	62	theme	good	1414:1417	arg1	stability					1419:1427	The good stability	1410:1427	The good stability of the sorbent	1410:1442	The good stability of the sorbent enabled up to eight cycles of reuse.Graphical abstract.					
33245436	7	63	theme	Under optimized	1036:1050	arg1	condition					1052:1060	Under optimized condition	1036:1060	Under optimized condition	1036:1060	Under optimized condition, the developed method provided good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1 for mevinphos, dimethoate, and parathion methyl, respectively.					
33245436	5	64	theme	composite	772:780	arg1	sorbent					782:788	The fabricated porous composite sorbent	750:788	The fabricated porous composite sorbent	750:788	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	7	65	theme	0.03-200 μg L-1	1114:1128	arg1	range					1105:1109	good linear range	1093:1109	good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1	1093:1190	Under optimized condition, the developed method provided good linear range of 0.03-200 μg L-1 for dichlorvos, malathion, and fenthion, and 0.075-200 μg L-1 for mevinphos, dimethoate, and parathion methyl, respectively.					
33245436	2	66	theme	composite	378:386	arg1	sorbent					388:394	The hierarchical porous composite sorbent	354:394	The hierarchical porous composite sorbent	354:394	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	1	67	used	used	258:261	arg2	sorbent					232:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	1	67	used	used	258:261	arg2	phase					274:278	a solid phase	266:278	a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides	266:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	3	68	theme	alginate	537:544	arg1	beads					555:559	the alginate hydrogel beads	533:559	the alginate hydrogel beads	533:559	The pores in the alginate hydrogel beads were produced by carbon dioxide bubbles from the reaction of incorporated calcium carbonate with hydrochloric acid.					
33245436	5	69	theme	best	887:890	arg1	performance					903:913	the best extraction performance	883:913	the best extraction performance	883:913	The fabricated porous composite sorbent was characterized and sorbent fabrication and extraction conditions were optimized to obtain the best extraction performance.					
33245436	4	70	theme	porous	681:686	arg1	network					688:694	The porous network	677:694	The porous network	677:694	The porous network was filled with dichloromethane to assist extraction.					
33245436	2	71	theme	thin	424:427	arg1	film					429:432	polypyrrole thin film	412:432	polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles	412:517	The hierarchical porous composite sorbent was composed of polypyrrole thin film coated on the surface of porous alginate beads with embedded magnetite nanoparticles.					
33245436	1	72	theme	solid	268:272	arg1	sorbent					232:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent	204:238	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33245436	1	72	theme	solid	268:272	arg1	phase					274:278	a solid phase	266:278	a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides	266:351	A porous composite magnetic sorbent was developed and used as a solid phase for the solvent-assisted preconcentration of organophosphorus pesticides.					
33407116	6	0	dep	rosette	1249:1255	arg1	leaves					1257:1262	leaves	1257:1262	leaves	1257:1262	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	8	1	theme	reagent	1638:1644	arg1	effect					1614:1619	the growth inhibitory effect	1592:1619	the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs	1592:1706	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	2	2	theme	plant	370:374	arg1	growth					376:381	various plant growth and development processes	362:407	growth	376:381	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	7	3	theme	galt	1488:1491	arg1	mutants					1493:1499	all the higher-order galt mutants	1467:1499	all the higher-order galt mutants	1467:1499	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	4	4	theme	biological	868:877	arg1	functions					879:887	their biological functions	862:887	their biological functions	862:887	Due to gene redundancy among the GALTs, single or double galt genetic knockout mutants are often not sufficient to fully reveal their biological functions.					
33407116	6	5	theme	galt2	1166:1170	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	5	theme	galt2	1166:1170	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	11	6	theme	mutant	2258:2263	arg1	lines					2265:2269	T-DNA mutant lines	2252:2269	T-DNA mutant lines	2252:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	10	7	theme	gal6	1949:1952	arg1	mutants					1954:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	12	8	from	AGPs	2454:2457	arg1	plants					2462:2467	plants	2462:2467	plants	2462:2467	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	10	9	theme	galt4	1937:1941	arg1	mutants					1954:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	6	10	theme	galt4	1149:1153	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	10	theme	galt4	1149:1153	arg1	galt6					1155:1159	galt3 galt4 galt6	1143:1159	galt3 galt4 galt6	1143:1159	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	11	11	theme	simpler	2119:2125	arg1	way					2138:2140	a simpler and faster way	2117:2140	a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines	2117:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	6	12	theme	glycosylated	1228:1239	arg1	AGPs					1241:1244	significantly less glycosylated AGPs	1209:1244	significantly less glycosylated AGPs in rosette leaves	1209:1262	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	1	13	theme	proteins	247:254	arg1	Arabinogalactan-proteins					180:203	BACKGROUND Arabinogalactan-proteins	169:203	BACKGROUND Arabinogalactan-proteins (AGPs)	169:210	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	1	13	theme	proteins	247:254	arg1	class					218:222	a class	216:222	a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs)	216:336	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	11	14	theme	gene	2073:2076	arg1	approach					2099:2106	Our CRISPR/Cas9 gene editing/multiplexing approach	2057:2106	Our CRISPR/Cas9 gene editing/multiplexing approach	2057:2106	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	6	15	theme	wild-type	1315:1323	arg1	organs					1325:1330	the corresponding wild-type organs	1297:1330	the corresponding wild-type organs	1297:1330	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	11	16	theme	lines	2265:2269	arg1	crossing					2240:2247	conventional genetic crossing	2219:2247	conventional genetic crossing of T-DNA mutant lines	2219:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	12	17	theme	biological	2419:2428	arg1	functions					2430:2438	the various biological functions	2407:2438	the various biological functions attributed to AGPs in plants	2407:2467	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	9	18	theme	galt2	1726:1730	arg1	mutant					1755:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	10	19	contain	had	1962:1964	arg1	mutants					1954:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	10	19	contain	had	1962:1964	arg2	defects					1966:1972	defects	1966:1972	defects in the female gametophyte which were responsible for reduced seed set	1966:2042	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	10	20	theme	female	1981:1986	arg1	gametophyte					1988:1998	the female gametophyte	1977:1998	the female gametophyte which were responsible for reduced seed set	1977:2042	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	10	20	theme	female	1981:1986	arg1	responsible					2011:2021	responsible	2011:2021	responsible	2011:2021	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	1	21	theme	BACKGROUND	169:178	arg1	Arabinogalactan-proteins					180:203	BACKGROUND Arabinogalactan-proteins	169:203	BACKGROUND Arabinogalactan-proteins (AGPs)	169:210	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	1	21	theme	BACKGROUND	169:178	arg1	class					218:222	a class	216:222	a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs)	216:336	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	1	21	theme	BACKGROUND	169:178	arg1	AGPs					206:209	AGPs	206:209	AGPs	206:209	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	9	22	theme	galt4	1738:1742	arg1	mutant					1755:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	7	23	theme	composition	1348:1358	arg1	analysis					1360:1367	Monosaccharide composition analysis	1333:1367	Monosaccharide composition analysis of AGPs isolated from rosette leaves	1333:1404	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	10	24	theme	reduced	2027:2033	arg1	set					2040:2042	reduced seed set	2027:2042	reduced seed set	2027:2042	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	5	25	theme	gene	1063:1066	arg1	GALT2-6					1076:1082	GALT2-6	1076:1082	GALT2-6	1076:1082	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	5	25	theme	gene	1063:1066	arg1	family					1068:1073	the GALT gene family	1054:1073	the GALT gene family (GALT2-6)	1054:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	2	26	theme	male	515:518	arg1	development					543:553	male and female gametophyte development	515:553	male and female gametophyte development	515:553	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	6	27	theme	galt4	1178:1182	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	27	theme	galt4	1178:1182	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	0	28	theme	family	88:93	arg1	editing					29:35	CRISPR-Cas9 multiplex genome editing	0:35	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family	0:93	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	12	29	dep	mutants	2290:2296	arg1	characterized					2311:2323	characterized	2311:2323	characterized in this study	2311:2337	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	12	29	dep	mutants	2290:2296	arg1	produced					2298:2305	produced	2298:2305	produced	2298:2305	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	7	30	from	decreases	1427:1435	arg1	arabinose					1440:1448	arabinose	1440:1448	arabinose	1440:1448	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	7	30	from	decreases	1427:1435	arg1	galactose					1454:1462	galactose	1454:1462	galactose	1454:1462	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	7	30	from	decreases	1427:1435	arg1	mutants					1493:1499	all the higher-order galt mutants	1467:1499	all the higher-order galt mutants	1467:1499	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	11	31	theme	T-DNA	2252:2256	arg1	lines					2265:2269	T-DNA mutant lines	2252:2269	T-DNA mutant lines	2252:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	8	32	theme	Phenotypic	1502:1511	arg1	analyses					1513:1520	Phenotypic analyses	1502:1520	Phenotypic analyses	1502:1520	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	0	33	theme	arabinogalactan-protein	102:124	arg1	glycosylation					126:138	arabinogalactan-protein glycosylation	102:138	arabinogalactan-protein glycosylation	102:138	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	12	34	theme	sugar	2385:2389	arg1	decorations					2391:2401	sugar decorations	2385:2401	sugar decorations	2385:2401	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	11	35	dep	CONCLUSIONS	2045:2055	arg1	provides					2108:2115	provides	2108:2115	provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines	2108:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	9	36	theme	overall	1780:1786	arg1	growth					1788:1793	reduced overall growth	1772:1793	reduced overall growth	1772:1793	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	12	37	theme	galt	2285:2288	arg1	mutants					2290:2296	Higher-order galt mutants	2272:2296	Higher-order galt mutants produced and characterized in this study	2272:2337	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	10	38	from	defects	1966:1972	arg1	gametophyte					1988:1998	the female gametophyte	1977:1998	the female gametophyte which were responsible for reduced seed set	1977:2042	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	10	38	from	defects	1966:1972	arg1	responsible					2011:2021	responsible	2011:2021	responsible	2011:2021	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	5	39	theme	editing/multiplexing	961:980	arg1	technology					982:991	CRISPR-Cas9 gene editing/multiplexing technology	944:991	CRISPR-Cas9 gene editing/multiplexing technology	944:991	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	2	40	theme	female	524:529	arg1	development					543:553	male and female gametophyte development	515:553	male and female gametophyte development	515:553	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	0	41	gly	glycosylation	126:138	arg1	Arabidopsis					156:166	Arabidopsis	156:166	Arabidopsis	156:166	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	9	42	theme	root	1805:1808	arg1	growth					1810:1815	impaired root growth	1796:1815	impaired root growth	1796:1815	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	9	43	theme	gal6	1750:1753	arg1	mutant					1755:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	5	44	dep	RESULTS	890:896	arg1	report					907:912	report	907:912	report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6)	907:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	0	45	theme	CRISPR-Cas9	0:10	arg1	editing					29:35	CRISPR-Cas9 multiplex genome editing	0:35	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family	0:93	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	11	46	theme	conventional	2219:2230	arg1	crossing					2240:2247	conventional genetic crossing	2219:2247	conventional genetic crossing of T-DNA mutant lines	2219:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	11	47	theme	functional	2179:2188	arg1	characterization					2190:2205	functional characterization	2179:2205	functional characterization compared to conventional genetic crossing of T-DNA mutant lines	2179:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	4	48	theme	gene	741:744	arg1	redundancy					746:755	gene redundancy	741:755	gene redundancy among the GALTs	741:771	Due to gene redundancy among the GALTs, single or double galt genetic knockout mutants are often not sufficient to fully reveal their biological functions.					
33407116	0	49	theme	genome	22:27	arg1	editing					29:35	CRISPR-Cas9 multiplex genome editing	0:35	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family	0:93	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	5	50	theme	CRISPR-Cas9	944:954	arg1	technology					982:991	CRISPR-Cas9 gene editing/multiplexing technology	944:991	CRISPR-Cas9 gene editing/multiplexing technology	944:991	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	8	51	theme	genes	1565:1569	arg1	mutation					1536:1543	mutation	1536:1543	mutation of two or more GALT genes	1536:1569	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	4	52	theme	knockout	804:811	arg1	mutants					813:819	single or double galt genetic knockout mutants	774:819	single or double galt genetic knockout mutants	774:819	Due to gene redundancy among the GALTs, single or double galt genetic knockout mutants are often not sufficient to fully reveal their biological functions.					
33407116	2	53	theme	somatic	435:441	arg1	embryogenesis					443:455	somatic embryogenesis	435:455	somatic embryogenesis	435:455	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	6	54	from	siliques	1276:1283	arg1	rosette					1249:1255	rosette leaves	1249:1262	rosette leaves	1249:1262	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	2	55	theme	salt	480:483	arg1	tolerance					485:493	salt tolerance	480:493	salt tolerance	480:493	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	56	theme	various	362:368	arg1	growth					376:381	various plant growth and development processes	362:407	growth	376:381	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	0	57	theme	hydroxyproline-O-galactosyltransferase	44:81	arg1	family					88:93	the hydroxyproline-O-galactosyltransferase gene family	40:93	the hydroxyproline-O-galactosyltransferase gene family	40:93	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	6	58	theme	galt3	1172:1176	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	58	theme	galt3	1172:1176	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	10	59	theme	Reciprocal	1875:1884	arg1	experiments					1895:1905	Reciprocal crossing experiments	1875:1905	Reciprocal crossing experiments	1875:1905	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	6	60	dep	mutants	1121:1127	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	60	dep	mutants	1121:1127	arg1	galt5					1136:1140	galt2 galt5	1130:1140	galt2 galt5	1130:1140	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	60	dep	mutants	1121:1127	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	60	dep	mutants	1121:1127	arg1	galt6					1155:1159	galt3 galt4 galt6	1143:1159	galt3 galt4 galt6	1143:1159	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	5	61	theme	higher-order	1005:1016	arg1	mutants					1027:1033	higher-order knockout mutants	1005:1033	higher-order knockout mutants of five members of the GALT gene family (GALT2-6)	1005:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	6	62	theme	galt	1116:1119	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	62	theme	galt	1116:1119	arg1	galt5					1136:1140	galt2 galt5	1130:1140	galt2 galt5	1130:1140	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	62	theme	galt	1116:1119	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	62	theme	galt	1116:1119	arg1	galt6					1155:1159	galt3 galt4 galt6	1143:1159	galt3 galt4 galt6	1143:1159	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	9	63	theme	reduced	1857:1863	arg1	set					1870:1872	reduced seed set	1857:1872	reduced seed set	1857:1872	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	8	64	theme	growth	1596:1601	arg1	effect					1614:1619	the growth inhibitory effect	1592:1619	the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs	1592:1706	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	4	65	theme	double	784:789	arg1	mutants					813:819	single or double galt genetic knockout mutants	774:819	single or double galt genetic knockout mutants	774:819	Due to gene redundancy among the GALTs, single or double galt genetic knockout mutants are often not sufficient to fully reveal their biological functions.					
33407116	2	66	theme	development	387:397	arg1	root					458:461	root	458:461	root	458:461	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	expansion					424:432	cell expansion	419:432	cell expansion	419:432	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	development					543:553	male and female gametophyte development	515:553	male and female gametophyte development	515:553	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	signaling					504:512	hormone signaling	496:512	hormone signaling	496:512	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	embryogenesis					443:455	somatic embryogenesis	435:455	somatic embryogenesis	435:455	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	defense					560:566	defense	560:566	defense	560:566	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	processes					399:407	various plant growth and development processes	362:407	processes	399:407	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	2	66	theme	development	387:397	arg1	tolerance					485:493	salt tolerance	480:493	salt tolerance	480:493	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	9	67	theme	shorter	1835:1841	arg1	siliques					1843:1850	shorter siliques	1835:1850	shorter siliques	1835:1850	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	10	68	theme	galt3	1931:1935	arg1	mutants					1954:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	6	69	from	AGPs	1241:1244	arg1	rosette					1249:1255	rosette leaves	1249:1262	rosette leaves	1249:1262	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	7	70	theme	higher-order	1475:1486	arg1	mutants					1493:1499	all the higher-order galt mutants	1467:1499	all the higher-order galt mutants	1467:1499	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	6	71	theme	AGPs	1086:1089	arg1	analysis					1091:1098	AGPs analysis	1086:1098	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1086:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	8	72	theme	β-D-Gal-Yariv	1624:1636	arg1	reagent					1638:1644	β-D-Gal-Yariv reagent	1624:1644	β-D-Gal-Yariv reagent	1624:1644	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	3	73	theme	first	699:703	arg1	sugar					705:709	the first sugar	695:709	the first sugar	695:709	To date, eight Hyp-O-galactosyltransferases (GALT2-6, HPGT1-3) have been identified; these enzymes are responsible for adding the first sugar, galactose, onto AGPs.					
33407116	3	73	theme	first	699:703	arg1	galactose					712:720	galactose	712:720	galactose	712:720	To date, eight Hyp-O-galactosyltransferases (GALT2-6, HPGT1-3) have been identified; these enzymes are responsible for adding the first sugar, galactose, onto AGPs.					
33407116	11	74	theme	faster	2131:2136	arg1	way					2138:2140	a simpler and faster way	2117:2140	a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines	2117:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	10	75	theme	galt5	1943:1947	arg1	mutants					1954:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	3	76	dep	Hyp-O-galactosyltransferases	584:611	arg1	GALT2-6					614:620	GALT2-6	614:620	GALT2-6	614:620	To date, eight Hyp-O-galactosyltransferases (GALT2-6, HPGT1-3) have been identified; these enzymes are responsible for adding the first sugar, galactose, onto AGPs.					
33407116	3	76	dep	Hyp-O-galactosyltransferases	584:611	arg1	HPGT1-3					623:629	HPGT1-3	623:629	HPGT1-3	623:629	To date, eight Hyp-O-galactosyltransferases (GALT2-6, HPGT1-3) have been identified; these enzymes are responsible for adding the first sugar, galactose, onto AGPs.					
33407116	11	77	theme	genetic	2232:2238	arg1	crossing					2240:2247	conventional genetic crossing	2219:2247	conventional genetic crossing of T-DNA mutant lines	2219:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	9	78	theme	reduced	1772:1778	arg1	growth					1788:1793	reduced overall growth	1772:1793	reduced overall growth	1772:1793	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	1	79	theme	hydroxyproline-rich	227:245	arg1	HRGPs					257:261	HRGPs	257:261	HRGPs	257:261	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	1	79	theme	hydroxyproline-rich	227:245	arg1	proteins					247:254	hydroxyproline-rich proteins	227:254	hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs)	227:336	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	11	80	theme	higher-order	2154:2165	arg1	mutants					2167:2173	higher-order mutants	2154:2173	higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines	2154:2269	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	6	81	theme	galt3	1143:1147	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	81	theme	galt3	1143:1147	arg1	galt6					1155:1159	galt3 galt4 galt6	1143:1159	galt3 galt4 galt6	1143:1159	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	7	82	theme	significant	1415:1425	arg1	decreases					1427:1435	significant decreases	1415:1435	significant decreases in arabinose and galactose in all the higher-order galt mutants	1415:1499	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	11	83	theme	editing/multiplexing	2078:2097	arg1	approach					2099:2106	Our CRISPR/Cas9 gene editing/multiplexing approach	2057:2106	Our CRISPR/Cas9 gene editing/multiplexing approach	2057:2106	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	1	84	dep	glycosylated	281:292	arg1	%					299:299	> 90%	295:299	> 90%	295:299	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	6	85	theme	corresponding	1301:1313	arg1	organs					1325:1330	the corresponding wild-type organs	1297:1330	the corresponding wild-type organs	1297:1330	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	7	86	attach	isolated	1377:1384	arg1	rosette					1391:1397	rosette leaves	1391:1404	rosette leaves	1391:1404	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	7	86	attach	isolated	1377:1384	arg2	analysis					1360:1367	Monosaccharide composition analysis	1333:1367	Monosaccharide composition analysis of AGPs isolated from rosette leaves	1333:1404	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	12	87	theme	various	2411:2417	arg1	functions					2430:2438	the various biological functions	2407:2438	the various biological functions attributed to AGPs in plants	2407:2467	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	9	88	theme	galt3	1732:1736	arg1	mutant					1755:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	7	89	theme	AGPs	1372:1375	arg1	analysis					1360:1367	Monosaccharide composition analysis	1333:1367	Monosaccharide composition analysis of AGPs isolated from rosette leaves	1333:1404	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	5	90	theme	GALT	1058:1061	arg1	GALT2-6					1076:1082	GALT2-6	1076:1082	GALT2-6	1076:1082	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	5	90	theme	GALT	1058:1061	arg1	family					1068:1073	the GALT gene family	1054:1073	the GALT gene family (GALT2-6)	1054:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	6	91	gly	glycosylated	1228:1239	arg1	AGPs					1241:1244	significantly less glycosylated AGPs	1209:1244	significantly less glycosylated AGPs in rosette leaves	1209:1262	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	9	92	theme	galt5	1744:1748	arg1	mutant					1755:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	the galt2 galt3 galt4 galt5 gal6 mutant	1722:1760	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	0	93	from	function	144:151	arg1	Arabidopsis					156:166	Arabidopsis	156:166	Arabidopsis	156:166	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	6	94	theme	galt5	1184:1188	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	94	theme	galt5	1184:1188	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	4	95	theme	single	774:779	arg1	mutants					813:819	single or double galt genetic knockout mutants	774:819	single or double galt genetic knockout mutants	774:819	Due to gene redundancy among the GALTs, single or double galt genetic knockout mutants are often not sufficient to fully reveal their biological functions.					
33407116	5	96	theme	family	1068:1073	arg1	members					1043:1049	five members	1038:1049	five members of the GALT gene family (GALT2-6)	1038:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	7	97	dep	rosette	1391:1397	arg1	leaves					1399:1404	leaves	1399:1404	leaves	1399:1404	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	7	98	theme	Monosaccharide	1333:1346	arg1	analysis					1360:1367	Monosaccharide composition analysis	1333:1367	Monosaccharide composition analysis of AGPs isolated from rosette leaves	1333:1404	Monosaccharide composition analysis of AGPs isolated from rosette leaves revealed significant decreases in arabinose and galactose in all the higher-order galt mutants.					
33407116	2	99	theme	hormone	496:502	arg1	signaling					504:512	hormone signaling	496:512	hormone signaling	496:512	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	1	100	dep	type	307:310	arg1	arabinogalactans					315:330	arabinogalactans	315:330	type II arabinogalactans (AGs)	307:336	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	1	100	dep	type	307:310	arg1	AGs					333:335	AGs	333:335	AGs	333:335	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	0	101	from	glycosylation	126:138	arg1	Arabidopsis					156:166	Arabidopsis	156:166	Arabidopsis	156:166	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	5	102	theme	gene	956:959	arg1	technology					982:991	CRISPR-Cas9 gene editing/multiplexing technology	944:991	CRISPR-Cas9 gene editing/multiplexing technology	944:991	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	2	103	theme	gametophyte	531:541	arg1	development					543:553	male and female gametophyte development	515:553	male and female gametophyte development	515:553	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	12	104	theme	Higher-order	2272:2283	arg1	mutants					2290:2296	Higher-order galt mutants	2272:2296	Higher-order galt mutants produced and characterized in this study	2272:2337	Higher-order galt mutants produced and characterized in this study provide insight into the relationship between sugar decorations and the various biological functions attributed to AGPs in plants.					
33407116	10	105	theme	seed	2035:2038	arg1	set					2040:2042	reduced seed set	2027:2042	reduced seed set	2027:2042	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	1	106	gly	glycosylated	281:292	arg1	HRGPs					257:261	HRGPs	257:261	HRGPs	257:261	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	1	106	gly	glycosylated	281:292	arg1	proteins					247:254	hydroxyproline-rich proteins	227:254	hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs)	227:336	BACKGROUND Arabinogalactan-proteins (AGPs) are a class of hydroxyproline-rich proteins (HRGPs) that are heavily glycosylated (> 90%) with type II arabinogalactans (AGs).					
33407116	5	107	theme	technology	982:991	arg1	application					929:939	the successful application	914:939	the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6)	914:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	9	108	theme	impaired	1796:1803	arg1	growth					1810:1815	impaired root growth	1796:1815	impaired root growth	1796:1815	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	2	109	theme	cell	419:422	arg1	expansion					424:432	cell expansion	419:432	cell expansion	419:432	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	0	110	theme	multiplex	12:20	arg1	editing					29:35	CRISPR-Cas9 multiplex genome editing	0:35	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family	0:93	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	5	111	theme	successful	918:927	arg1	application					929:939	the successful application	914:939	the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6)	914:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	2	112	theme	stem	467:470	arg1	growth					472:477	stem growth	467:477	stem growth	467:477	AGPs are implicated in various plant growth and development processes including cell expansion, somatic embryogenesis, root and stem growth, salt tolerance, hormone signaling, male and female gametophyte development, and defense.					
33407116	8	113	theme	GALT	1560:1563	arg1	genes					1565:1569	two or more GALT genes	1548:1569	two or more GALT genes	1548:1569	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	9	114	theme	abnormal	1818:1825	arg1	pollen					1827:1832	abnormal pollen	1818:1832	abnormal pollen	1818:1832	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	8	115	from	backbones	1690:1698	arg1	AGPs					1703:1706	AGPs	1703:1706	AGPs	1703:1706	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	4	116	theme	galt genetic	791:802	arg1	mutants					813:819	single or double galt genetic knockout mutants	774:819	single or double galt genetic knockout mutants	774:819	Due to gene redundancy among the GALTs, single or double galt genetic knockout mutants are often not sufficient to fully reveal their biological functions.					
33407116	0	117	theme	gene	83:86	arg1	family					88:93	the hydroxyproline-O-galactosyltransferase gene family	40:93	the hydroxyproline-O-galactosyltransferase gene family	40:93	CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.					
33407116	5	118	theme	members	1043:1049	arg1	mutants					1027:1033	higher-order knockout mutants	1005:1033	higher-order knockout mutants of five members of the GALT gene family (GALT2-6)	1005:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	6	119	theme	galt2	1130:1134	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	119	theme	galt2	1130:1134	arg1	galt5					1136:1140	galt2 galt5	1130:1140	galt2 galt5	1130:1140	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	11	120	theme	CRISPR/Cas9	2061:2071	arg1	approach					2099:2106	Our CRISPR/Cas9 gene editing/multiplexing approach	2057:2106	Our CRISPR/Cas9 gene editing/multiplexing approach	2057:2106	CONCLUSIONS Our CRISPR/Cas9 gene editing/multiplexing approach provides a simpler and faster way to generate higher-order mutants for functional characterization compared to conventional genetic crossing of T-DNA mutant lines.					
33407116	10	121	theme	crossing	1886:1893	arg1	experiments					1895:1905	Reciprocal crossing experiments	1875:1905	Reciprocal crossing experiments	1875:1905	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	6	122	theme	mutants	1121:1127	arg1	analysis					1091:1098	AGPs analysis	1086:1098	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1086:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	8	123	theme	inhibitory	1603:1612	arg1	effect					1614:1619	the growth inhibitory effect	1592:1619	the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs	1592:1706	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	8	124	theme	β-1,3-galactan	1675:1688	arg1	backbones					1690:1698	β-1,3-galactan backbones	1675:1698	β-1,3-galactan backbones on AGPs	1675:1706	Phenotypic analyses revealed that mutation of two or more GALT genes was able to overcome the growth inhibitory effect of β-D-Gal-Yariv reagent, which specifically binds to β-1,3-galactan backbones on AGPs.					
33407116	5	125	theme	knockout	1018:1025	arg1	mutants					1027:1033	higher-order knockout mutants	1005:1033	higher-order knockout mutants of five members of the GALT gene family (GALT2-6)	1005:1083	RESULTS Here, we report the successful application of CRISPR-Cas9 gene editing/multiplexing technology to generate higher-order knockout mutants of five members of the GALT gene family (GALT2-6).					
33407116	6	126	theme	higher-order	1103:1114	arg1	mutants					1121:1127	higher-order galt mutants	1103:1127	higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6)	1103:1194	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	126	theme	higher-order	1103:1114	arg1	galt5					1136:1140	galt2 galt5	1130:1140	galt2 galt5	1130:1140	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	126	theme	higher-order	1103:1114	arg1	gal6					1190:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	galt2 galt3 galt4 galt5 gal6	1166:1193	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	6	126	theme	higher-order	1103:1114	arg1	galt6					1155:1159	galt3 galt4 galt6	1143:1159	galt3 galt4 galt6	1143:1159	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
33407116	9	127	theme	seed	1865:1868	arg1	set					1870:1872	reduced seed set	1857:1872	reduced seed set	1857:1872	In addition, the galt2 galt3 galt4 galt5 gal6 mutant exhibited reduced overall growth, impaired root growth, abnormal pollen, shorter siliques, and reduced seed set.					
33407116	10	128	theme	galt2	1925:1929	arg1	mutants					1954:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	galt2 galt3 galt4 galt5 gal6 mutants	1925:1960	Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects in the female gametophyte which were responsible for reduced seed set.					
33407116	6	129	from	stems	1265:1269	arg1	rosette					1249:1255	rosette leaves	1249:1262	rosette leaves	1249:1262	AGPs analysis of higher-order galt mutants (galt2 galt5, galt3 galt4 galt6, and galt2 galt3 galt4 galt5 gal6) demonstrated significantly less glycosylated AGPs in rosette leaves, stems, and siliques compared to the corresponding wild-type organs.					
31841999	8	0	theme	rat	1155:1157	arg1	cells					1168:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	4	1	theme	hydrogel	571:578	arg1	neurocompatibility					509:526	the neurocompatibility	505:526	the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture	505:619	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	10	2	theme	functional	1695:1704	arg1	activity					1716:1723	the functional metabolic activity	1691:1723	the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity	1691:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	1	3	theme	brain	228:232	arg1	engineering					241:251	brain tissue engineering	228:251	brain tissue engineering	228:251	Over the last decade, a number of hydrogels attracted great attention in the area of brain tissue engineering.					
31841999	4	4	theme	primary	585:591	arg1	culture					613:619	primary rat cortical neuron culture	585:619	primary rat cortical neuron culture	585:619	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	5	5	theme	molding	653:659	arg1	technique					661:669	a molding technique	651:669	a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959	651:831	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	10	6	theme	cortical	1741:1748	arg1	cells					1750:1754	neonatal rat cortical cells	1728:1754	neonatal rat cortical cells	1728:1754	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	1	7	theme	engineering	241:251	arg1	area					220:223	the area	216:223	the area of brain tissue engineering	216:251	Over the last decade, a number of hydrogels attracted great attention in the area of brain tissue engineering.					
31841999	6	8	theme	cell	867:870	arg1	viability					872:880	cell viability	867:880	cell viability	867:880	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	10	9	theme	neonatal	1728:1735	arg1	cells					1750:1754	neonatal rat cortical cells	1728:1754	neonatal rat cortical cells	1728:1754	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	6	10	theme	hydrogel	855:862	arg1	influence					838:846	The influence	834:846	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity	834:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	2	11	theme	hydrophilic	284:294	arg1	polymers					296:303	hydrophilic polymers	284:303	hydrophilic polymers forming 3D network in water	284:331	The hydrogels are composed of hydrophilic polymers forming 3D network in water.					
31841999	8	12	theme	hydrogel	1205:1212	arg1	surface					1214:1220	the hydrogel surface	1201:1220	the hydrogel surface	1201:1220	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	6	13	theme	consumption	953:963	arg1	rate					965:968	oxygen consumption rate	946:968	oxygen consumption rate	946:968	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	10	14	from	activity	1716:1723	arg1	response					1759:1766	response	1759:1766	response to glutamate excitotoxicity	1759:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	8	15	theme	mitochondrial	1275:1287	arg1	μM					1360:1361	100 μM	1356:1361	100 μM	1356:1361	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	8	15	theme	mitochondrial	1275:1287	arg1	function					1289:1296	mitochondrial function	1275:1296	mitochondrial function in both standard conditions	1275:1324	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	5	16	theme	photocurable	686:697	arg1	composition					699:709	photocurable composition	686:709	photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959	686:831	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	4	17	with	neurocompatibility	509:526	arg1	culture					613:619	primary rat cortical neuron culture	585:619	primary rat cortical neuron culture	585:619	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	1	18	theme	hydrogels	177:185	arg1	number					167:172	a number	165:172	a number of hydrogels	165:185	Over the last decade, a number of hydrogels attracted great attention in the area of brain tissue engineering.					
31841999	0	19	theme	feasibility	119:129	arg1	evaluation					131:140	in vitro feasibility evaluation	110:140	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.	0:141	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	8	20	from	homeostasis	1259:1269	arg1	conditions					1315:1324	both standard conditions	1301:1324	both standard conditions	1301:1324	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	1	21	theme	great	197:201	arg1	attention					203:211	great attention	197:211	great attention	197:211	Over the last decade, a number of hydrogels attracted great attention in the area of brain tissue engineering.					
31841999	6	22	theme	mitochondrial	918:930	arg1	potential					932:940	mitochondrial potential	918:940	mitochondrial potential	918:940	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	8	23	theme	standard	1306:1313	arg1	conditions					1315:1324	both standard conditions	1301:1324	both standard conditions	1301:1324	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	6	24	theme	primary	1017:1023	arg1	cultures					1032:1039	primary neuron cultures	1017:1039	primary neuron cultures obtained from a neonatal rat cortex	1017:1075	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	10	25	from	response	1759:1766	arg1	realization					1676:1686	realization	1676:1686	realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity	1676:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	0	26	theme	tissue	61:66	arg1	regeneration					68:79	nervous tissue regeneration	53:79	nervous tissue regeneration	53:79	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	1	27	theme	last	152:155	arg1	decade					157:162	the last decade	148:162	the last decade	148:162	Over the last decade, a number of hydrogels attracted great attention in the area of brain tissue engineering.					
31841999	9	28	dep	chitosan-g-oligo	1501:1516	arg1	L-lactide					1520:1528	L-lactide	1520:1528	L-lactide	1520:1528	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	9	28	dep	chitosan-g-oligo	1501:1516	arg1	L					1518:1518	L	1518:1518	L	1518:1518	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	9	29	theme	hydrogel	1541:1548	arg1	application					1486:1496	the application	1482:1496	the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury	1482:1595	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	9	30	theme	tissue	1554:1559	arg1	engineering					1561:1571	tissue engineering	1554:1571	tissue engineering therapy	1554:1579	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	3	31	theme	structural	362:371	arg1	restoration					388:398	structural and functional restoration	362:398	structural and functional restoration of damaged brain tissues	362:423	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	8	32	theme	control	1379:1385	arg1	cells					1387:1391	the control cells	1375:1391	the control cells cultured without the hydrogel	1375:1421	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	3	33	theme	brain	411:415	arg1	tissues					417:423	damaged brain tissues	403:423	damaged brain tissues	403:423	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	0	34	theme	Chitosan-g-oligo	0:15	arg1	hydrogel					40:47	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel	0:47	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.	0:141	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	8	35	from	excitotoxicity	1340:1353	arg1	conditions					1315:1324	both standard conditions	1301:1324	both standard conditions	1301:1324	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	6	36	theme	neonatal	1057:1064	arg1	cortex					1070:1075	a neonatal rat cortex	1055:1075	a neonatal rat cortex	1055:1075	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	0	37	dep	hydrogel	40:47	arg1	evaluation					131:140	in vitro feasibility evaluation	110:140	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.	0:141	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	9	38	theme	brain	1584:1588	arg1	injury					1590:1595	brain injury	1584:1595	brain injury	1584:1595	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	6	39	from	rate	965:968	arg1	excitotoxicity					983:996	glutamate excitotoxicity	973:996	glutamate excitotoxicity	973:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	3	40	theme	mechanical	438:447	arg1	support					449:455	mechanical support	438:455	mechanical support	438:455	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	5	41	theme	poly	766:769	arg1	diacrylate					788:797	poly(ethylene glycol) diacrylate	766:797	poly(ethylene glycol) diacrylate	766:797	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	3	42	theme	functional	377:386	arg1	restoration					388:398	structural and functional restoration	362:398	structural and functional restoration of damaged brain tissues	362:423	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	5	43	theme	ethylene	771:778	arg1	poly					766:769	poly	766:769	poly(ethylene glycol) diacrylate	766:797	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	5	43	theme	ethylene	771:778	arg1	glycol					780:785	ethylene glycol	771:785	ethylene glycol	771:785	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	9	44	theme	in	1603:1604	arg1	model					1612:1616	an in vitro model	1600:1616	an in vitro model	1600:1616	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	4	45	theme	chitosan-g-oligo	531:546	arg1	hydrogel					571:578	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	531:578	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	531:578	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	6	46	from	potential	932:940	arg1	excitotoxicity					983:996	glutamate excitotoxicity	973:996	glutamate excitotoxicity	973:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	2	47	theme	3D	313:314	arg1	network					316:322	3D network	313:322	3D network	313:322	The hydrogels are composed of hydrophilic polymers forming 3D network in water.					
31841999	4	48	theme	rat	593:595	arg1	culture					613:619	primary rat cortical neuron culture	585:619	primary rat cortical neuron culture	585:619	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	6	49	from	phenotype	883:891	arg1	excitotoxicity					983:996	glutamate excitotoxicity	973:996	glutamate excitotoxicity	973:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	5	50	from	technique	661:669	arg1	base					678:681	the base	674:681	the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959	674:831	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	6	51	theme	calcium	897:903	arg1	homeostasis					905:915	calcium homeostasis	897:915	calcium homeostasis	897:915	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	0	52	dep	Chitosan-g-oligo	0:15	arg1	L					17:17	L	17:17	L	17:17	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	0	52	dep	Chitosan-g-oligo	0:15	arg1	L-lactide					19:27	L-lactide	19:27	L-lactide	19:27	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	4	53	theme	neuron	606:611	arg1	culture					613:619	primary rat cortical neuron culture	585:619	primary rat cortical neuron culture	585:619	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	10	54	theme	metabolic	1706:1714	arg1	activity					1716:1723	the functional metabolic activity	1691:1723	the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity	1691:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	8	55	theme	neonatal	1146:1153	arg1	cells					1168:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	10	56	from	realization	1676:1686	arg1	response					1759:1766	response	1759:1766	response to glutamate excitotoxicity	1759:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	4	57	theme	copolymer	561:569	arg1	hydrogel					571:578	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	531:578	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	531:578	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	10	58	theme	cells	1750:1754	arg1	activity					1716:1723	the functional metabolic activity	1691:1723	the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity	1691:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	1	59	theme	tissue	234:239	arg1	engineering					241:251	brain tissue engineering	228:251	brain tissue engineering	228:251	Over the last decade, a number of hydrogels attracted great attention in the area of brain tissue engineering.					
31841999	8	60	theme	cortical	1159:1166	arg1	cells					1168:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	6	61	from	viability	872:880	arg1	excitotoxicity					983:996	glutamate excitotoxicity	973:996	glutamate excitotoxicity	973:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	10	62	theme	rat	1737:1739	arg1	cells					1750:1754	neonatal rat cortical cells	1728:1754	neonatal rat cortical cells	1728:1754	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	0	63	theme	copolymer	30:38	arg1	hydrogel					40:47	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel	0:47	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.	0:141	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	9	64	dep	in	1603:1604	arg1	vitro					1606:1610	vitro	1606:1610	vitro	1606:1610	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	6	65	from	influence	838:846	arg1	viability					872:880	cell viability	867:880	cell viability	867:880	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	6	65	from	influence	838:846	arg1	potential					932:940	mitochondrial potential	918:940	mitochondrial potential	918:940	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	6	65	from	influence	838:846	arg1	phenotype					883:891	phenotype	883:891	phenotype	883:891	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	6	65	from	influence	838:846	arg1	rate					965:968	oxygen consumption rate	946:968	oxygen consumption rate	946:968	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	10	66	theme	glutamate	1771:1779	arg1	excitotoxicity					1781:1794	glutamate excitotoxicity	1771:1794	glutamate excitotoxicity	1771:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
31841999	8	67	theme	calcium	1251:1257	arg1	homeostasis					1259:1269	calcium homeostasis	1251:1269	calcium homeostasis	1251:1269	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	5	68	theme	photosensitizer	803:817	arg1	Irgacure					819:826	photosensitizer Irgacure 2959	803:831	photosensitizer Irgacure 2959	803:831	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	6	69	theme	oxygen	946:951	arg1	rate					965:968	oxygen consumption rate	946:968	oxygen consumption rate	946:968	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	0	70	dep	in	110:111	arg1	vitro					113:117	vitro	113:117	vitro	113:117	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	5	71	theme	composition	699:709	arg1	base					678:681	the base	674:681	the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959	674:831	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	9	72	theme	application	1486:1496	arg1	feasibility					1467:1477	the feasibility	1463:1477	the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model	1463:1616	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	8	73	from	phenotype	1240:1248	arg1	conditions					1315:1324	both standard conditions	1301:1324	both standard conditions	1301:1324	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	9	74	theme	chitosan-g-oligo	1501:1516	arg1	hydrogel					1541:1548	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	1501:1548	chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury	1501:1595	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	6	75	theme	neuron	1025:1030	arg1	cultures					1032:1039	primary neuron cultures	1017:1039	primary neuron cultures obtained from a neonatal rat cortex	1017:1075	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	5	76	dep	chitosan-g-oligo	725:740	arg1	L-lactide					744:752	L-lactide	744:752	L-lactide	744:752	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	5	76	dep	chitosan-g-oligo	725:740	arg1	L					742:742	L	742:742	L	742:742	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	5	76	dep	chitosan-g-oligo	725:740	arg1	diacrylate					788:797	poly(ethylene glycol) diacrylate	766:797	poly(ethylene glycol) diacrylate	766:797	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	5	76	dep	chitosan-g-oligo	725:740	arg1	Irgacure					819:826	photosensitizer Irgacure 2959	803:831	photosensitizer Irgacure 2959	803:831	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	5	76	dep	chitosan-g-oligo	725:740	arg1	copolymer					755:763	copolymer	755:763	copolymer	755:763	The hydrogel was produced by a molding technique on the base of photocurable composition consisting of chitosan-g-oligo(L,L-lactide) copolymer, poly(ethylene glycol) diacrylate and photosensitizer Irgacure 2959.					
31841999	3	77	theme	cell	472:475	arg1	fate					477:480	cell fate	472:480	cell fate	472:480	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	0	78	theme	nervous	53:59	arg1	regeneration					68:79	nervous tissue regeneration	53:79	nervous tissue regeneration	53:79	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	8	79	from	function	1289:1296	arg1	conditions					1315:1324	both standard conditions	1301:1324	both standard conditions	1301:1324	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	8	80	theme	glutamate	1330:1338	arg1	excitotoxicity					1340:1353	glutamate excitotoxicity	1330:1353	glutamate excitotoxicity	1330:1353	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	4	81	dep	chitosan-g-oligo	531:546	arg1	L-lactide					550:558	L-lactide	550:558	L-lactide	550:558	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	4	81	dep	chitosan-g-oligo	531:546	arg1	L					548:548	L	548:548	L	548:548	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	0	82	theme	in	110:111	arg1	evaluation					131:140	in vitro feasibility evaluation	110:140	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.	0:141	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	0	83	theme	glutamate	84:92	arg1	excitotoxicity					94:107	glutamate excitotoxicity	84:107	glutamate excitotoxicity	84:107	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	9	84	theme	copolymer	1531:1539	arg1	hydrogel					1541:1548	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	1501:1548	chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury	1501:1595	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	6	85	theme	glutamate	973:981	arg1	excitotoxicity					983:996	glutamate excitotoxicity	973:996	glutamate excitotoxicity	973:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	0	86	from	hydrogel	40:47	arg1	excitotoxicity					94:107	glutamate excitotoxicity	84:107	glutamate excitotoxicity	84:107	Chitosan-g-oligo(L,L-lactide) copolymer hydrogel for nervous tissue regeneration in glutamate excitotoxicity: in vitro feasibility evaluation.					
31841999	8	87	theme	similar	1364:1370	arg1	phenotype					1240:1248	the phenotype	1236:1248	the phenotype	1236:1248	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	9	88	theme	engineering	1561:1571	arg1	therapy					1573:1579	tissue engineering therapy	1554:1579	tissue engineering therapy	1554:1579	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	6	89	theme	rat	1066:1068	arg1	cortex					1070:1075	a neonatal rat cortex	1055:1075	a neonatal rat cortex	1055:1075	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	3	90	theme	tissues	417:423	arg1	restoration					388:398	structural and functional restoration	362:398	structural and functional restoration of damaged brain tissues	362:423	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	9	91	theme	injury	1590:1595	arg1	hydrogel					1541:1548	chitosan-g-oligo(L,L-lactide) copolymer hydrogel	1501:1548	chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury	1501:1595	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	3	92	theme	damaged	403:409	arg1	tissues					417:423	damaged brain tissues	403:423	damaged brain tissues	403:423	Their function is promoting structural and functional restoration of damaged brain tissues by providing mechanical support and navigating cell fate.					
31841999	6	93	from	homeostasis	905:915	arg1	excitotoxicity					983:996	glutamate excitotoxicity	973:996	glutamate excitotoxicity	973:996	The influence of the hydrogel on cell viability, phenotype and calcium homeostasis, mitochondrial potential and oxygen consumption rate in glutamate excitotoxicity was analyzed using primary neuron cultures obtained from a neonatal rat cortex.					
31841999	9	94	from	feasibility	1467:1477	arg1	model					1612:1616	an in vitro model	1600:1616	an in vitro model	1600:1616	To conclude, in this study we assessed the feasibility of the application of chitosan-g-oligo(L,L-lactide) copolymer hydrogel for tissue engineering therapy of brain injury in an in vitro model.					
31841999	4	95	theme	cortical	597:604	arg1	culture					613:619	primary rat cortical neuron culture	585:619	primary rat cortical neuron culture	585:619	This paper reports on the neurocompatibility of chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture.					
31841999	8	96	theme	Dissociated	1134:1144	arg1	cells					1168:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells	1134:1172	Dissociated neonatal rat cortical cells were actively attaching to the hydrogel surface and exhibited the phenotype, calcium homeostasis and mitochondrial function in both standard conditions and glutamate excitotoxicity (100 μM) similar to the control cells cultured without the hydrogel.					
31841999	10	97	theme	activity	1716:1723	arg1	realization					1676:1686	realization	1676:1686	realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity	1676:1794	The results support that the hydrogel is able to sustain realization of the functional metabolic activity of neonatal rat cortical cells in response to glutamate excitotoxicity.					
33502752	6	0	used	used	901:904	arg2	GM					867:868	The GM	863:868	The GM obtained through extraction	863:896	The GM obtained through extraction is used to study its enzyme inhibitory property associated with hyperglycaemia.					
33502752	6	1	theme	enzyme	919:924	arg1	property					937:944	its enzyme inhibitory property	915:944	its enzyme inhibitory property associated with hyperglycaemia	915:975	The GM obtained through extraction is used to study its enzyme inhibitory property associated with hyperglycaemia.					
33502752	5	2	dep	Fourier	654:660	arg1	transform					662:670	transform	662:670	transform infrared	662:679	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	5	3	theme	scanning	695:702	arg1	calorimetry					704:714	differential scanning calorimetry	682:714	differential scanning calorimetry	682:714	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	7	4	theme	GM	986:987	arg1	yield					989:993	RESULTS GM yield	978:993	RESULTS GM yield extracted from the husk	978:1017	RESULTS GM yield extracted from the husk is highly significant compared to other groups.					
33502752	2	5	theme	seed	346:349	arg1	GM					351:352	fenugreek seed GM	336:352	fenugreek seed GM	336:352	In this study, selective extraction is adapted to extract fenugreek seed GM to improvise the yield recovery.					
33502752	11	6	theme	resources	1497:1505	arg1	use					1490:1492	minimal use	1482:1492	minimal use of resources	1482:1505	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	11	6	theme	resources	1497:1505	arg1	compound					1472:1479	the bioactive compound	1458:1479	the bioactive compound	1458:1479	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	5	7	theme	scanning	717:724	arg1	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy	717:744	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	11	8	theme	industrial	1521:1530	arg1	viability					1532:1540	industrial viability	1521:1540	industrial viability	1521:1540	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	13	9	theme	enzyme	1604:1609	arg1	property					1622:1629	Its enzyme inhibitory property	1600:1629	Its enzyme inhibitory property	1600:1629	Its enzyme inhibitory property makes GM a valuable compound in diabetic prevention/treatment.					
33502752	5	10	theme	electron	726:733	arg1	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy	717:744	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	4	11	theme	Comparative	464:474	arg1	studies					476:482	Comparative studies	464:482	Comparative studies	464:482	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	8	12	theme	GM	1085:1086	arg1	yield					1088:1092	pure GM yield	1080:1092	pure GM yield	1080:1092	Crude GM and pure GM yield was 2 and 3.25 times higher than that obtained through whole seed samples.					
33502752	13	13	theme	inhibitory	1611:1620	arg1	property					1622:1629	Its enzyme inhibitory property	1600:1629	Its enzyme inhibitory property	1600:1629	Its enzyme inhibitory property makes GM a valuable compound in diabetic prevention/treatment.					
33502752	0	14	theme	enzyme	102:107	arg1	potential					120:128	its enzyme inhibitory potential	98:128	its enzyme inhibitory potential associated with hyperglycaemia	98:159	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia.					
33502752	7	15	theme	RESULTS	978:984	arg1	yield					989:993	RESULTS GM yield	978:993	RESULTS GM yield extracted from the husk	978:1017	RESULTS GM yield extracted from the husk is highly significant compared to other groups.					
33502752	2	16	theme	yield	371:375	arg1	recovery					377:384	the yield recovery	367:384	the yield recovery	367:384	In this study, selective extraction is adapted to extract fenugreek seed GM to improvise the yield recovery.					
33502752	5	17	dep	transform	662:670	arg1	infrared					672:679	infrared	672:679	transform infrared	662:679	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	0	18	from	fenugreek	43:51	arg1	extraction					10:19	Selective extraction	0:19	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.)	0:82	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia.					
33502752	8	19	theme	Crude	1067:1071	arg1	GM					1073:1074	Crude GM	1067:1074	Crude GM	1067:1074	Crude GM and pure GM yield was 2 and 3.25 times higher than that obtained through whole seed samples.					
33502752	9	20	theme	pure	1197:1200	arg1	GM					1202:1203	the pure GM	1193:1203	the pure GM	1193:1203	The characterization of the pure GM is on a par with the existing reports.					
33502752	1	21	theme	host	189:192	arg1	compounds					212:220	host various bioactive compounds	189:220	host various bioactive compounds	189:220	BACKGROUND Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre.					
33502752	5	22	theme	optical	778:784	arg1	density					786:792	optical density	778:792	optical density	778:792	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	8	23	theme	pure	1080:1083	arg1	yield					1088:1092	pure GM yield	1080:1092	pure GM yield	1080:1092	Crude GM and pure GM yield was 2 and 3.25 times higher than that obtained through whole seed samples.					
33502752	9	24	theme	GM	1202:1203	arg1	characterization					1173:1188	The characterization	1169:1188	The characterization of the pure GM	1169:1203	The characterization of the pure GM is on a par with the existing reports.					
33502752	1	25	dep	BACKGROUND	162:171	arg1	seeds					183:187	Fenugreek seeds	173:187	Fenugreek seeds	173:187	BACKGROUND Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre.					
33502752	1	25	dep	BACKGROUND	162:171	arg1	compounds					212:220	host various bioactive compounds	189:220	host various bioactive compounds	189:220	BACKGROUND Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre.					
33502752	10	26	theme	purified	1248:1255	arg1	GM					1257:1258	The purified GM	1244:1258	The purified GM	1244:1258	The purified GM inhibited α-amylase and α-glucosidase enzymes in vitro, with an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 μg mL-1 , respectively.					
33502752	1	27	theme	various	194:200	arg1	compounds					212:220	host various bioactive compounds	189:220	host various bioactive compounds	189:220	BACKGROUND Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre.					
33502752	0	28	theme	Selective	0:8	arg1	extraction					10:19	Selective extraction	0:19	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.)	0:82	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia.					
33502752	11	29	theme	CONCLUSION	1387:1396	arg1	extraction					1408:1417	CONCLUSION Selective extraction	1387:1417	CONCLUSION Selective extraction	1387:1417	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	1	30	theme	bioactive	202:210	arg1	compounds					212:220	host various bioactive compounds	189:220	host various bioactive compounds	189:220	BACKGROUND Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre.					
33502752	0	31	theme	galactomannan	24:36	arg1	extraction					10:19	Selective extraction	0:19	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.)	0:82	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia.					
33502752	4	32	theme	GM	535:536	arg1	yield					538:542	the crude and pure GM yield	516:542	the crude and pure GM yield between different groups such as the whole seed, and the classified fractions	516:620	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	5	33	theme	differential	682:693	arg1	calorimetry					704:714	differential scanning calorimetry	682:714	differential scanning calorimetry	682:714	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	10	34	theme	67.17 ± 5.15 μg mL-1	1350:1369	arg1	IC50					1324:1327	an IC50	1321:1327	an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 μg mL-1	1321:1369	The purified GM inhibited α-amylase and α-glucosidase enzymes in vitro, with an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 μg mL-1 , respectively.					
33502752	2	35	theme	selective	293:301	arg1	extraction					303:312	selective extraction	293:312	selective extraction	293:312	In this study, selective extraction is adapted to extract fenugreek seed GM to improvise the yield recovery.					
33502752	11	36	theme	compound	1472:1479	arg1	recovery					1446:1453	the recovery	1442:1453	the recovery of the bioactive compound, minimal use of resources	1442:1505	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	4	37	theme	classified	601:610	arg1	fractions					612:620	the classified fractions	597:620	the classified fractions	597:620	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	10	38	theme	21.08 ± 0.085	1332:1344	arg1	IC50					1324:1327	an IC50	1321:1327	an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 μg mL-1	1321:1369	The purified GM inhibited α-amylase and α-glucosidase enzymes in vitro, with an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 μg mL-1 , respectively.					
33502752	4	39	theme	different	552:560	arg1	fractions					612:620	the classified fractions	597:620	the classified fractions	597:620	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	4	39	theme	different	552:560	arg1	seed					587:590	seed	587:590	seed	587:590	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	4	39	theme	different	552:560	arg1	groups					562:567	different groups	552:567	different groups such as the whole seed, and the classified fractions	552:620	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	0	40	theme	inhibitory	109:118	arg1	potential					120:128	its enzyme inhibitory potential	98:128	its enzyme inhibitory potential associated with hyperglycaemia	98:159	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia.					
33502752	13	41	theme	diabetic	1663:1670	arg1	prevention/treatment					1672:1691	diabetic prevention/treatment	1663:1691	diabetic prevention/treatment	1663:1691	Its enzyme inhibitory property makes GM a valuable compound in diabetic prevention/treatment.					
33502752	1	42	theme	Fenugreek	173:181	arg1	seeds					183:187	Fenugreek seeds	173:187	Fenugreek seeds	173:187	BACKGROUND Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre.					
33502752	10	43	theme	α-glucosidase	1284:1296	arg1	enzymes					1298:1304	α-glucosidase enzymes	1284:1304	α-glucosidase enzymes	1284:1304	The purified GM inhibited α-amylase and α-glucosidase enzymes in vitro, with an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 μg mL-1 , respectively.					
33502752	5	44	theme	magnetic	843:850	arg1	resonance					852:860	nuclear magnetic resonance	835:860	nuclear magnetic resonance	835:860	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	0	45	dep	Trigonella	54:63	arg1	L.					80:81	Trigonella foenum-graecum L.	54:81	Trigonella foenum-graecum L.	54:81	Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia.					
33502752	9	46	with	par	1213:1215	arg1	reports					1235:1241	the existing reports	1222:1241	the existing reports	1222:1241	The characterization of the pure GM is on a par with the existing reports.					
33502752	4	47	theme	crude	520:524	arg1	yield					538:542	the crude and pure GM yield	516:542	the crude and pure GM yield between different groups such as the whole seed, and the classified fractions	516:620	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	11	48	theme	Selective	1398:1406	arg1	extraction					1408:1417	CONCLUSION Selective extraction	1387:1417	CONCLUSION Selective extraction	1387:1417	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	8	49	theme	seed	1155:1158	arg1	samples					1160:1166	whole seed samples	1149:1166	whole seed samples	1149:1166	Crude GM and pure GM yield was 2 and 3.25 times higher than that obtained through whole seed samples.					
33502752	14	50	theme	Chemical	1712:1719	arg1	Industry					1721:1728	Chemical Industry	1712:1728	Chemical Industry	1712:1728	© 2021 Society of Chemical Industry.					
33502752	2	51	theme	fenugreek	336:344	arg1	GM					351:352	fenugreek seed GM	336:352	fenugreek seed GM	336:352	In this study, selective extraction is adapted to extract fenugreek seed GM to improvise the yield recovery.					
33502752	4	52	theme	pure	530:533	arg1	yield					538:542	the crude and pure GM yield	516:542	the crude and pure GM yield between different groups such as the whole seed, and the classified fractions	516:620	Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions.					
33502752	9	53	theme	existing	1226:1233	arg1	reports					1235:1241	the existing reports	1222:1241	the existing reports	1222:1241	The characterization of the pure GM is on a par with the existing reports.					
33502752	11	54	theme	minimal	1482:1488	arg1	use					1490:1492	minimal use	1482:1492	minimal use of resources	1482:1505	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	11	54	theme	minimal	1482:1488	arg1	compound					1472:1479	the bioactive compound	1458:1479	the bioactive compound	1458:1479	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	13	55	theme	valuable	1642:1649	arg1	compound					1651:1658	a valuable compound	1640:1658	a valuable compound in diabetic prevention/treatment	1640:1691	Its enzyme inhibitory property makes GM a valuable compound in diabetic prevention/treatment.					
33502752	11	56	from	enhancement	1427:1437	arg1	recovery					1446:1453	the recovery	1442:1453	the recovery of the bioactive compound, minimal use of resources	1442:1505	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	7	57	theme	other	1053:1057	arg1	groups					1059:1064	other groups	1053:1064	other groups	1053:1064	RESULTS GM yield extracted from the husk is highly significant compared to other groups.					
33502752	13	58	from	compound	1651:1658	arg1	prevention/treatment					1672:1691	diabetic prevention/treatment	1663:1691	diabetic prevention/treatment	1663:1691	Its enzyme inhibitory property makes GM a valuable compound in diabetic prevention/treatment.					
33502752	5	59	theme	monosaccharide	747:760	arg1	composition					762:772	monosaccharide composition	747:772	monosaccharide composition	747:772	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	5	60	theme	nuclear	835:841	arg1	resonance					852:860	nuclear magnetic resonance	835:860	nuclear magnetic resonance	835:860	Characterization is done using Fourier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance.					
33502752	11	61	theme	bioactive	1462:1470	arg1	use					1490:1492	minimal use	1482:1492	minimal use of resources	1482:1505	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	11	61	theme	bioactive	1462:1470	arg1	compound					1472:1479	the bioactive compound	1458:1479	the bioactive compound	1458:1479	CONCLUSION Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability.					
33502752	6	62	theme	inhibitory	926:935	arg1	property					937:944	its enzyme inhibitory property	915:944	its enzyme inhibitory property associated with hyperglycaemia	915:975	The GM obtained through extraction is used to study its enzyme inhibitory property associated with hyperglycaemia.					
33502752	12	63	theme	compound	1567:1574	arg1	Characterization					1543:1558	Characterization	1543:1558	Characterization of the compound	1543:1574	Characterization of the compound confirms the structure.					
33502752	8	64	theme	whole	1149:1153	arg1	samples					1160:1166	whole seed samples	1149:1166	whole seed samples	1149:1166	Crude GM and pure GM yield was 2 and 3.25 times higher than that obtained through whole seed samples.					
34959989	7	0	theme	prostaglandin	1059:1071	arg1	E2					1078:1079	prostaglandin (PG) E2	1059:1079	prostaglandin (PG) E2 from arachidonic acid	1059:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	3	1	theme	oil	445:447	arg1	OOE					459:461	OOE	459:461	OOE	459:461	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	1	theme	oil	445:447	arg1	extracts					449:456	olive oil extracts	439:456	olive oil extracts (OOE)	439:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	2	theme	inflammatory	467:478	arg1	mediators					480:488	inflammatory mediators	467:488	inflammatory mediators	467:488	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	8	3	theme	phospholipid	1205:1216	arg1	composition					1221:1231	the phospholipid FA composition	1201:1231	the phospholipid FA composition	1201:1231	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	3	4	from	oil	421:423	arg1	acids					399:403	major fatty acids	387:403	major fatty acids (FA) from olive oil with those of olive oil extracts (OOE)	387:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	4	from	oil	421:423	arg1	effects					376:382	the effects	372:382	the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages	372:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	4	from	oil	421:423	arg1	FA					406:407	FA	406:407	FA	406:407	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	5	5	theme	synthase	829:836	arg1	expression					792:801	the LPS-induced expression	776:801	the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA	776:890	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	5	6	theme	mRNA	887:890	arg1	expression					792:801	the LPS-induced expression	776:801	the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA	776:890	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	1	7	theme	oleic	192:196	arg1	OA					204:205	OA	204:205	OA	204:205	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	7	theme	oleic	192:196	arg1	acid					198:201	oleic acid	192:201	oleic acid (OA)	192:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	0	8	from	Response	73:80	arg1	Macrophages					101:111	Murine RAW264.7 Macrophages	85:111	Murine RAW264.7 Macrophages	85:111	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	4	9	theme	different	590:598	arg1	OOE					600:602	different OOE	590:602	different OOE	590:602	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	7	10	theme	nitric	996:1001	arg1	oxide					1003:1007	nitric oxide	996:1007	nitric oxide	996:1007	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	5	11	theme	nitric	816:821	arg1	synthase					829:836	inducible nitric oxide synthase	806:836	inducible nitric oxide synthase (iNos)	806:843	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	5	11	theme	nitric	816:821	arg1	iNos					839:842	iNos	839:842	iNos	839:842	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	7	12	with	treatment	1133:1141	arg1	OA					1155:1156	OA	1155:1156	OA	1155:1156	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	7	12	with	treatment	1133:1141	arg1	OOE					1148:1150	OOE	1148:1150	OOE	1148:1150	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	4	13	theme	-stimulated	648:658	arg1	macrophages					676:686	lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	624:686	lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	624:686	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	0	14	theme	Prostaglandin	139:151	arg1	E2					153:154	Prostaglandin E2	139:154	Prostaglandin E2	139:154	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	3	15	theme	cellular	513:520	arg1	composition					535:545	the cellular phospholipid composition	509:545	the cellular phospholipid composition in murine macrophages	509:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	4	16	theme	RAW264.7	667:674	arg1	macrophages					676:686	lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	624:686	lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	624:686	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	9	17	theme	acid	1425:1428	arg1	release					1402:1408	the release	1398:1408	the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA	1398:1481	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	9	17	theme	acid	1425:1428	arg1	incorporation					1499:1511	competitive incorporation	1487:1511	competitive incorporation of fatty acids into membrane phospholipids	1487:1554	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	8	18	theme	arachidonic	1238:1248	arg1	acid					1250:1253	arachidonic acid	1238:1253	arachidonic acid	1238:1253	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	0	19	theme	LPS-Induced	48:58	arg1	Response					73:80	the LPS-Induced Inflammatory Response	44:80	the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages	44:111	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	3	20	theme	murine	550:555	arg1	macrophages					557:567	murine macrophages	550:567	murine macrophages	550:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	9	21	theme	hereinafter	1434:1444	arg1	PGE2					1446:1449	hereinafter PGE2	1434:1449	hereinafter PGE2	1434:1449	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	6	22	theme	iNos	941:944	arg1	expression					954:963	iNos protein expression	941:963	iNos protein expression	941:963	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	5	23	theme	Olive	728:732	arg1	extracts					738:745	Olive oil extracts	728:745	Olive oil extracts	728:745	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	3	24	from	effects	376:382	arg1	oil					421:423	olive oil	415:423	olive oil with those of olive oil extracts (OOE)	415:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	24	from	effects	376:382	arg1	alterations					494:504	alterations	494:504	alterations in the cellular phospholipid composition in murine macrophages	494:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	24	from	effects	376:382	arg1	mediators					480:488	inflammatory mediators	467:488	inflammatory mediators	467:488	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	1	25	theme	Olive	157:161	arg1	oil					163:165	Olive oil	157:165	Olive oil	157:165	Olive oil contains high amounts of oleic acid (OA).					
34959989	0	26	theme	Murine	85:90	arg1	Macrophages					101:111	Murine RAW264.7 Macrophages	85:111	Murine RAW264.7 Macrophages	85:111	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	2	27	theme	inflammatory	251:262	arg1	processes					264:272	inflammatory processes	251:272	inflammatory processes	251:272	Although OA has been described to inhibit inflammatory processes, the effects of olive oil on cellular mechanisms remain poorly understood.					
34959989	9	28	theme	due	1459:1461	arg1	acid					1425:1428	arachidonic acid	1413:1428	arachidonic acid	1413:1428	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	8	29	theme	FA	1218:1219	arg1	composition					1221:1231	the phospholipid FA composition	1201:1231	the phospholipid FA composition	1201:1231	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	3	30	theme	acids	399:403	arg1	effects					376:382	the effects	372:382	the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages	372:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	8	31	theme	elevated	1278:1285	arg1	availability					1287:1298	an elevated availability	1275:1298	an elevated availability of arachidonic acid	1275:1318	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	3	32	theme	major	387:391	arg1	acids					399:403	major fatty acids	387:403	major fatty acids (FA) from olive oil with those of olive oil extracts (OOE)	387:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	32	theme	major	387:391	arg1	FA					406:407	FA	406:407	FA	406:407	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	0	33	theme	Olive	0:4	arg1	Oil					6:8	Olive Oil	0:8	Olive Oil Extracts	0:17	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	9	34	theme	OA	1480:1481	arg1	elongation					1466:1475	elongation	1466:1475	elongation of OA	1466:1481	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	5	35	theme	LPS-induced	780:790	arg1	expression					792:801	the LPS-induced expression	776:801	the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA	776:890	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	9	36	theme	competitive	1487:1497	arg1	incorporation					1499:1511	competitive incorporation	1487:1511	competitive incorporation of fatty acids into membrane phospholipids	1487:1554	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	8	37	theme	acid	1315:1318	arg1	availability					1287:1298	an elevated availability	1275:1298	an elevated availability of arachidonic acid	1275:1318	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	3	38	from	composition	535:545	arg1	macrophages					557:567	murine macrophages	550:567	murine macrophages	550:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	0	39	theme	Oleic	23:27	arg1	Acid					29:32	Oleic Acid	23:32	Oleic Acid	23:32	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	7	40	theme	E2	1078:1079	arg1	formation					1046:1054	the formation	1042:1054	the formation of prostaglandin (PG) E2 from arachidonic acid	1042:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	7	41	from	formation	1046:1054	arg1	acid					1098:1101	arachidonic acid	1086:1101	arachidonic acid	1086:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	2	42	theme	cellular	303:310	arg1	mechanisms					312:321	cellular mechanisms	303:321	cellular mechanisms	303:321	Although OA has been described to inhibit inflammatory processes, the effects of olive oil on cellular mechanisms remain poorly understood.					
34959989	7	43	theme	PG	1074:1075	arg1	E2					1078:1079	prostaglandin (PG) E2	1059:1079	prostaglandin (PG) E2 from arachidonic acid	1059:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	9	44	theme	acids	1522:1526	arg1	release					1402:1408	the release	1398:1408	the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA	1398:1481	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	9	44	theme	acids	1522:1526	arg1	incorporation					1499:1511	competitive incorporation	1487:1511	competitive incorporation of fatty acids into membrane phospholipids	1487:1554	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	2	45	theme	oil	296:298	arg1	effects					279:285	the effects	275:285	the effects of olive oil on cellular mechanisms	275:321	Although OA has been described to inhibit inflammatory processes, the effects of olive oil on cellular mechanisms remain poorly understood.					
34959989	9	46	theme	membrane	1533:1540	arg1	phospholipids					1542:1554	membrane phospholipids	1533:1554	membrane phospholipids	1533:1554	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	9	47	theme	anti-inflammatory	1350:1366	arg1	effects					1368:1374	anti-inflammatory effects	1350:1374	anti-inflammatory effects	1350:1374	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	3	48	theme	olive	439:443	arg1	OOE					459:461	OOE	459:461	OOE	459:461	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	48	theme	olive	439:443	arg1	extracts					449:456	olive oil extracts	439:456	olive oil extracts (OOE)	439:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	9	49	theme	PGE2	1446:1449	arg1	release					1402:1408	the release	1398:1408	the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA	1398:1481	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	9	49	theme	PGE2	1446:1449	arg1	incorporation					1499:1511	competitive incorporation	1487:1511	competitive incorporation of fatty acids into membrane phospholipids	1487:1554	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	3	50	with	oil	421:423	arg1	those					430:434	those	430:434	those	430:434	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	5	51	theme	oxide	823:827	arg1	synthase					829:836	inducible nitric oxide synthase	806:836	inducible nitric oxide synthase (iNos)	806:843	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	5	51	theme	oxide	823:827	arg1	iNos					839:842	iNos	839:842	iNos	839:842	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	1	52	theme	high	176:179	arg1	OA					204:205	OA	204:205	OA	204:205	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	52	theme	high	176:179	arg1	amounts					181:187	high amounts	176:187	high amounts of oleic acid (OA)	176:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	52	theme	high	176:179	arg1	acid					198:201	oleic acid	192:201	oleic acid (OA)	192:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	6	53	theme	significant	908:918	arg1	decrease					920:927	a significant decrease	906:927	a significant decrease in Cox2 and iNos protein expression	906:963	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	4	54	theme	FA	605:606	arg1	compositions					608:619	FA compositions	605:619	FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	605:686	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	7	55	theme	oxide	1003:1007	arg1	formation					983:991	The formation	979:991	The formation of nitric oxide	979:1007	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	3	56	theme	phospholipid	522:533	arg1	composition					535:545	the cellular phospholipid composition	509:545	the cellular phospholipid composition in murine macrophages	509:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	5	57	theme	inducible	806:814	arg1	synthase					829:836	inducible nitric oxide synthase	806:836	inducible nitric oxide synthase (iNos)	806:843	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	5	57	theme	inducible	806:814	arg1	iNos					839:842	iNos	839:842	iNos	839:842	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	0	58	theme	E2	153:154	arg1	Release					128:134	the Release	124:134	the Release of Prostaglandin E2	124:154	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	3	59	from	mediators	480:488	arg1	composition					535:545	the cellular phospholipid composition	509:545	the cellular phospholipid composition in murine macrophages	509:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	9	60	theme	arachidonic	1413:1423	arg1	acid					1425:1428	arachidonic acid	1413:1428	arachidonic acid	1413:1428	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	4	61	theme	murine	660:665	arg1	macrophages					676:686	lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	624:686	lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	624:686	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	3	62	from	alterations	494:504	arg1	composition					535:545	the cellular phospholipid composition	509:545	the cellular phospholipid composition in murine macrophages	509:567	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	0	63	theme	Inflammatory	60:71	arg1	Response					73:80	the LPS-Induced Inflammatory Response	44:80	the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages	44:111	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	8	64	from	shift	1192:1196	arg1	composition					1221:1231	the phospholipid FA composition	1201:1231	the phospholipid FA composition	1201:1231	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	6	65	theme	protein	946:952	arg1	expression					954:963	iNos protein expression	941:963	iNos protein expression	941:963	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	5	66	theme	cyclooxygenase	846:859	arg1	expression					792:801	the LPS-induced expression	776:801	the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA	776:890	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	6	67	located	observed	969:976	arg2	decrease					920:927	a significant decrease	906:927	a significant decrease in Cox2 and iNos protein expression	906:963	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	6	67	located	observed	969:976	arg1	addition					896:903	addition	896:903	addition	896:903	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	0	68	theme	RAW264.7	92:99	arg1	Macrophages					101:111	Murine RAW264.7 Macrophages	85:111	Murine RAW264.7 Macrophages	85:111	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	2	69	from	effects	279:285	arg1	mechanisms					312:321	cellular mechanisms	303:321	cellular mechanisms	303:321	Although OA has been described to inhibit inflammatory processes, the effects of olive oil on cellular mechanisms remain poorly understood.					
34959989	4	70	theme	gas	708:710	arg1	chromatography					712:725	gas chromatography	708:725	gas chromatography	708:725	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	7	71	from	acid	1098:1101	arg1	formation					1046:1054	the formation	1042:1054	the formation of prostaglandin (PG) E2 from arachidonic acid	1042:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	7	71	from	acid	1098:1101	arg1	E2					1078:1079	prostaglandin (PG) E2	1059:1079	prostaglandin (PG) E2 from arachidonic acid	1059:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
34959989	6	72	from	decrease	920:927	arg1	Cox2					932:935	Cox2	932:935	Cox2	932:935	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	6	72	from	decrease	920:927	arg1	expression					954:963	iNos protein expression	941:963	iNos protein expression	941:963	In addition, a significant decrease in Cox2 and iNos protein expression was observed.					
34959989	0	73	theme	Oil	6:8	arg1	Extracts					10:17	Olive Oil Extracts	0:17	Olive Oil Extracts	0:17	Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2.					
34959989	8	74	from	acid	1250:1253	arg1	shift					1192:1196	a shift	1190:1196	a shift in the phospholipid FA composition from arachidonic acid to OA	1190:1259	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	5	75	theme	interleukin-6	873:885	arg1	mRNA					887:890	interleukin-6 mRNA	873:890	interleukin-6 mRNA	873:890	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	4	76	theme	macrophages	676:686	arg1	compositions					608:619	FA compositions	605:619	FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages	605:686	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	3	77	theme	fatty	393:397	arg1	acids					399:403	major fatty acids	387:403	major fatty acids (FA) from olive oil with those of olive oil extracts (OOE)	387:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	77	theme	fatty	393:397	arg1	FA					406:407	FA	406:407	FA	406:407	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	3	78	theme	olive	415:419	arg1	oil					421:423	olive oil	415:423	olive oil with those of olive oil extracts (OOE)	415:462	Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages.					
34959989	5	79	theme	oil	734:736	arg1	extracts					738:745	Olive oil extracts	728:745	Olive oil extracts	728:745	Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA.					
34959989	1	80	theme	acid	198:201	arg1	OA					204:205	OA	204:205	OA	204:205	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	80	theme	acid	198:201	arg1	amounts					181:187	high amounts	176:187	high amounts of oleic acid (OA)	176:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	80	theme	acid	198:201	arg1	acid					198:201	oleic acid	192:201	oleic acid (OA)	192:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	81	contain	contains	167:174	arg2	OA					204:205	OA	204:205	OA	204:205	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	81	contain	contains	167:174	arg1	oil					163:165	Olive oil	157:165	Olive oil	157:165	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	81	contain	contains	167:174	arg2	acid					198:201	oleic acid	192:201	oleic acid (OA)	192:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	1	81	contain	contains	167:174	arg2	amounts					181:187	high amounts	176:187	high amounts of oleic acid (OA)	176:206	Olive oil contains high amounts of oleic acid (OA).					
34959989	8	82	theme	arachidonic	1303:1313	arg1	acid					1315:1318	arachidonic acid	1303:1318	arachidonic acid	1303:1318	The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid.					
34959989	9	83	theme	fatty	1516:1520	arg1	acids					1522:1526	fatty acids	1516:1526	fatty acids	1516:1526	Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE2, likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids.					
34959989	4	84	with	treatment	575:583	arg1	OOE					600:602	different OOE	590:602	different OOE	590:602	Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography.					
34959989	2	85	theme	olive	290:294	arg1	oil					296:298	olive oil	290:298	olive oil	290:298	Although OA has been described to inhibit inflammatory processes, the effects of olive oil on cellular mechanisms remain poorly understood.					
34959989	7	86	theme	arachidonic	1086:1096	arg1	acid					1098:1101	arachidonic acid	1086:1101	arachidonic acid	1086:1101	The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E2 from arachidonic acid significantly increased after treatment with OOE or OA.					
33349214	10	0	theme	hybrid	1517:1522	arg1	spheres					1534:1540	amoxicillin loaded hybrid composite spheres	1498:1540	amoxicillin loaded hybrid composite spheres (22-29 mm)	1498:1551	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	10	0	theme	hybrid	1517:1522	arg1	mm					1549:1550	22-29 mm	1543:1550	22-29 mm	1543:1550	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	12	1	theme	potential	1714:1722	arg1	cytotoxicity					1724:1735	no potential cytotoxicity	1711:1735	no potential cytotoxicity with hybrid composite spheres	1711:1765	The MTT assay indicated no potential cytotoxicity with hybrid composite spheres.					
33349214	3	2	theme	Antimicrobial-susceptibility	582:609	arg1	assay					611:615	METHODS Antimicrobial-susceptibility assay	574:615	METHODS Antimicrobial-susceptibility assay on MRSA	574:623	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	12	3	theme	MTT	1691:1693	arg1	assay					1695:1699	The MTT assay	1687:1699	The MTT assay	1687:1699	The MTT assay indicated no potential cytotoxicity with hybrid composite spheres.					
33349214	7	4	theme	Ag	1082:1083	arg1	nanoparticles					1085:1097	Ag nanoparticles	1082:1097	Ag nanoparticles	1082:1097	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	2	5	theme	Synergistic	330:340	arg1	activity					352:359	OBJECTIVE Synergistic anti-MRSA activity	320:359	OBJECTIVE Synergistic anti-MRSA activity	320:359	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	3	6	theme	agar	660:663	arg1	techniques					674:683	agar dilution techniques	660:683	agar dilution techniques	660:683	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	8	7	dep	nanoparticles	1214:1226	arg1	formation					1241:1249	formation	1241:1249	formation	1241:1249	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	8	7	dep	nanoparticles	1214:1226	arg1	presence					1228:1235	presence	1228:1235	presence	1228:1235	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	8	7	dep	nanoparticles	1214:1226	arg1	nanoparticles					1214:1226	Ag nanoparticles presence and formation	1211:1249	Ag nanoparticles presence and formation	1211:1249	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	13	8	from	effect	1795:1800	arg1	study					1832:1836	the current study	1820:1836	the current study	1820:1836	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	1	9	theme	Incoherent	156:165	arg1	use					167:169	BACKGROUND Incoherent use	145:169	BACKGROUND Incoherent use of antibiotics	145:184	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	7	10	theme	spheres	1070:1076	arg1	diameter					1049:1056	The mean diameter	1040:1056	The mean diameter of chitosan spheres and Ag nanoparticles	1040:1097	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	7	10	theme	spheres	1070:1076	arg1	mm					1106:1107	02 mm	1103:1107	02 mm	1103:1107	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	2	11	theme	various	532:538	arg1	characterizations					540:556	various characterizations	532:556	various characterizations	532:556	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	0	12	theme	Material	65:72	arg1	Spheres					84:90	Hybrid Material Composite Spheres	58:90	Hybrid Material Composite Spheres	58:90	Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.					
33349214	0	13	theme	Biological	14:23	arg1	Evaluation					25:34	Biological Evaluation	14:34	Biological Evaluation	14:34	Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.					
33349214	1	14	from	resistance	201:210	arg1	MRSA					215:218	MRSA	215:218	MRSA	215:218	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	6	15	dep	128	1028:1030	arg1	to					1025:1026	to	1025:1026	to	1025:1026	MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1.					
33349214	4	16	theme	in	755:756	arg1	studies					781:787	The in vitro and cytotoxicity studies	751:787	The in vitro and cytotoxicity studies	751:787	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	6	17	dep	L-1	1035:1037	arg1	mg					1032:1033	mg	1032:1033	mg	1032:1033	MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1.					
33349214	4	18	theme	abrasion	816:823	arg1	model					831:835	the skin abrasion mouse model	807:835	the skin abrasion mouse model	807:835	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	13	19	theme	enhanced	1883:1890	arg1	activity					1902:1909	enhanced anti-MRSA activity	1883:1909	enhanced anti-MRSA activity	1883:1909	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	13	20	theme	promising	1849:1857	arg1	formulation					1866:1876	a promising hybrid formulation	1847:1876	a promising hybrid formulation with enhanced anti-MRSA activity	1847:1909	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	1	21	theme	hospital	301:308	arg1	settings					310:317	the community and hospital settings	283:317	settings	310:317	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	10	22	theme	anti-MRSA	1474:1482	arg1	activity					1484:1491	augmented anti-MRSA activity	1464:1491	augmented anti-MRSA activity	1464:1491	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	10	23	dep	in	1442:1443	arg1	vitro					1445:1449	vitro	1445:1449	vitro	1445:1449	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	1	24	theme	antibiotics	174:184	arg1	use					167:169	BACKGROUND Incoherent use	145:169	BACKGROUND Incoherent use of antibiotics	145:184	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	2	25	theme	synthesis	510:518	arg1	method					520:525	one-step synthesis method	501:525	one-step synthesis method	501:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	11	26	theme	composite	1656:1664	arg1	formulation					1674:1684	loaded hybrid composite spheres formulation	1642:1684	loaded hybrid composite spheres formulation	1642:1684	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	13	27	theme	current	1824:1830	arg1	study					1832:1836	the current study	1820:1836	the current study	1820:1836	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	2	28	theme	nanoparticles	449:461	arg1	spheres					422:428	hybrid material composite spheres	396:428	hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method	396:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	9	29	from	peaks	1395:1399	arg1	formulation					1425:1435	the formulation	1421:1435	the formulation	1421:1435	No substantial interface among drug, nanoparticles, and polymer was found in XRD, and IR showed characteristic peaks of all compounds in the formulation.					
33349214	3	30	used	used	716:719	arg2	diffusion					702:710	agar well diffusion	692:710	agar well diffusion	692:710	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	10	31	theme	loaded	1510:1515	arg1	spheres					1534:1540	amoxicillin loaded hybrid composite spheres	1498:1540	amoxicillin loaded hybrid composite spheres (22-29 mm)	1498:1551	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	10	31	theme	loaded	1510:1515	arg1	mm					1549:1550	22-29 mm	1543:1550	22-29 mm	1543:1550	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	11	32	theme	loaded	1642:1647	arg1	formulation					1674:1684	loaded hybrid composite spheres formulation	1642:1684	loaded hybrid composite spheres formulation	1642:1684	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	1	33	with	multidrug-resistant	230:248	arg1	rate					262:265	a high rate	255:265	a high rate of virulence	255:278	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	13	34	dep	CONCLUSION	1768:1777	arg1	predicts					1838:1845	predicts	1838:1845	predicts a promising hybrid formulation with enhanced anti-MRSA activity	1838:1909	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	3	35	theme	composite	732:740	arg1	spheres					742:748	hybrid composite spheres	725:748	hybrid composite spheres	725:748	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	4	36	theme	MTT	841:843	arg1	assay					845:849	MTT assay	841:849	MTT assay	841:849	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	11	37	theme	significant	1556:1566	arg1	reduction					1568:1576	A significant reduction	1554:1576	A significant reduction in microbial burden (~6.5 log10 CFU mL-1)	1554:1618	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	1	38	theme	high	257:260	arg1	rate					262:265	a high rate	255:265	a high rate of virulence	255:278	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	2	39	theme	composite	412:420	arg1	spheres					422:428	hybrid material composite spheres	396:428	hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method	396:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	8	40	theme	spheres	1149:1155	arg1	Morphology					1135:1144	Morphology	1135:1144	Morphology of spheres	1135:1155	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	8	41	from	SEM	1202:1204	arg1	uneven					1161:1166	uneven	1161:1166	uneven	1161:1166	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	10	42	theme	composite	1524:1532	arg1	spheres					1534:1540	amoxicillin loaded hybrid composite spheres	1498:1540	amoxicillin loaded hybrid composite spheres (22-29 mm)	1498:1551	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	10	42	theme	composite	1524:1532	arg1	mm					1549:1550	22-29 mm	1543:1550	22-29 mm	1543:1550	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	2	43	theme	hybrid	396:401	arg1	spheres					422:428	hybrid material composite spheres	396:428	hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method	396:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	9	44	theme	characteristic	1380:1393	arg1	peaks					1395:1399	characteristic peaks	1380:1399	characteristic peaks of all compounds in the formulation	1380:1435	No substantial interface among drug, nanoparticles, and polymer was found in XRD, and IR showed characteristic peaks of all compounds in the formulation.					
33349214	3	45	theme	well	697:700	arg1	diffusion					702:710	agar well diffusion	692:710	agar well diffusion	692:710	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	11	46	theme	CFU	1610:1612	arg1	mL-1					1614:1617	~6.5 log10 CFU mL-1	1599:1617	~6.5 log10 CFU mL-1	1599:1617	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	11	46	theme	CFU	1610:1612	arg1	burden					1591:1596	microbial burden	1581:1596	microbial burden (~6.5 log10 CFU mL-1)	1581:1618	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	2	47	theme	OBJECTIVE	320:328	arg1	activity					352:359	OBJECTIVE Synergistic anti-MRSA activity	320:359	OBJECTIVE Synergistic anti-MRSA activity	320:359	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	1	48	theme	community	287:295	arg1	settings					310:317	the community and hospital settings	283:317	settings	310:317	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	12	49	theme	hybrid	1742:1747	arg1	spheres					1759:1765	hybrid composite spheres	1742:1765	hybrid composite spheres	1742:1765	The MTT assay indicated no potential cytotoxicity with hybrid composite spheres.					
33349214	3	50	theme	METHODS	574:580	arg1	assay					611:615	METHODS Antimicrobial-susceptibility assay	574:615	METHODS Antimicrobial-susceptibility assay on MRSA	574:623	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	9	51	theme	compounds	1408:1416	arg1	peaks					1395:1399	characteristic peaks	1380:1399	characteristic peaks of all compounds in the formulation	1380:1435	No substantial interface among drug, nanoparticles, and polymer was found in XRD, and IR showed characteristic peaks of all compounds in the formulation.					
33349214	2	52	theme	anti-MRSA	342:350	arg1	activity					352:359	OBJECTIVE Synergistic anti-MRSA activity	320:359	OBJECTIVE Synergistic anti-MRSA activity	320:359	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	7	53	theme	nanoparticles	1085:1097	arg1	diameter					1049:1056	The mean diameter	1040:1056	The mean diameter of chitosan spheres and Ag nanoparticles	1040:1097	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	7	53	theme	nanoparticles	1085:1097	arg1	mm					1106:1107	02 mm	1103:1107	02 mm	1103:1107	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	11	54	from	reduction	1568:1576	arg1	mL-1					1614:1617	~6.5 log10 CFU mL-1	1599:1617	~6.5 log10 CFU mL-1	1599:1617	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	11	54	from	reduction	1568:1576	arg1	burden					1591:1596	microbial burden	1581:1596	microbial burden (~6.5 log10 CFU mL-1)	1581:1618	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	3	55	theme	disc	641:644	arg1	diffusion					646:654	disc diffusion	641:654	disc diffusion	641:654	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	1	56	theme	BACKGROUND	145:154	arg1	use					167:169	BACKGROUND Incoherent use	145:169	BACKGROUND Incoherent use of antibiotics	145:184	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
33349214	8	57	theme	Ag	1211:1212	arg1	formation					1241:1249	formation	1241:1249	formation	1241:1249	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	8	57	theme	Ag	1211:1212	arg1	presence					1228:1235	presence	1228:1235	presence	1228:1235	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	8	57	theme	Ag	1211:1212	arg1	nanoparticles					1214:1226	Ag nanoparticles presence and formation	1211:1249	Ag nanoparticles presence and formation	1211:1249	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	6	58	theme	high	985:988	arg1	resistance					990:999	high resistance	985:999	high resistance	985:999	MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1.					
33349214	13	59	theme	anti-MRSA	1892:1900	arg1	activity					1902:1909	enhanced anti-MRSA activity	1883:1909	enhanced anti-MRSA activity	1883:1909	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	7	60	theme	chitosan	1061:1068	arg1	spheres					1070:1076	chitosan spheres	1061:1076	chitosan spheres	1061:1076	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	7	61	theme	mean	1044:1047	arg1	diameter					1049:1056	The mean diameter	1040:1056	The mean diameter of chitosan spheres and Ag nanoparticles	1040:1097	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	7	61	theme	mean	1044:1047	arg1	mm					1106:1107	02 mm	1103:1107	02 mm	1103:1107	The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm, respectively.					
33349214	12	62	with	cytotoxicity	1724:1735	arg1	spheres					1759:1765	hybrid composite spheres	1742:1765	hybrid composite spheres	1742:1765	The MTT assay indicated no potential cytotoxicity with hybrid composite spheres.					
33349214	9	63	located	found	1352:1356	arg2	interface					1299:1307	No substantial interface	1284:1307	No substantial interface among drug, nanoparticles, and polymer	1284:1346	No substantial interface among drug, nanoparticles, and polymer was found in XRD, and IR showed characteristic peaks of all compounds in the formulation.					
33349214	9	63	located	found	1352:1356	arg1	XRD					1361:1363	XRD	1361:1363	XRD	1361:1363	No substantial interface among drug, nanoparticles, and polymer was found in XRD, and IR showed characteristic peaks of all compounds in the formulation.					
33349214	4	64	theme	cytotoxicity	768:779	arg1	studies					781:787	The in vitro and cytotoxicity studies	751:787	The in vitro and cytotoxicity studies	751:787	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	0	65	theme	Composite	74:82	arg1	Spheres					84:90	Hybrid Material Composite Spheres	58:90	Hybrid Material Composite Spheres	58:90	Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.					
33349214	3	66	theme	dilution	665:672	arg1	techniques					674:683	agar dilution techniques	660:683	agar dilution techniques	660:683	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	5	67	theme	tested	923:928	arg1	antibiotics					930:940	the tested antibiotics	919:940	the tested antibiotics	919:940	RESULTS All isolates showed resistance to the tested antibiotics except vancomycin.					
33349214	6	68	dep	mg	1032:1033	arg1	128					1028:1030	128	1028:1030	128	1028:1030	MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1.					
33349214	13	69	theme	hybrid	1859:1864	arg1	formulation					1866:1876	a promising hybrid formulation	1847:1876	a promising hybrid formulation with enhanced anti-MRSA activity	1847:1909	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	3	70	from	assay	611:615	arg1	MRSA					620:623	MRSA	620:623	MRSA	620:623	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	13	71	with	formulation	1866:1876	arg1	activity					1902:1909	enhanced anti-MRSA activity	1883:1909	enhanced anti-MRSA activity	1883:1909	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	9	72	theme	substantial	1287:1297	arg1	interface					1299:1307	No substantial interface	1284:1307	No substantial interface among drug, nanoparticles, and polymer	1284:1346	No substantial interface among drug, nanoparticles, and polymer was found in XRD, and IR showed characteristic peaks of all compounds in the formulation.					
33349214	4	73	dep	in	755:756	arg1	vitro					758:762	vitro	758:762	vitro	758:762	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	4	74	theme	skin	811:814	arg1	model					831:835	the skin abrasion mouse model	807:835	the skin abrasion mouse model	807:835	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	10	75	theme	in	1442:1443	arg1	assay					1451:1455	The in vitro assay	1438:1455	The in vitro assay	1438:1455	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	4	76	theme	mouse	825:829	arg1	model					831:835	the skin abrasion mouse model	807:835	the skin abrasion mouse model	807:835	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	2	77	theme	amoxicillin	433:443	arg1	spheres					422:428	hybrid material composite spheres	396:428	hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method	396:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	6	78	from	L-1	1035:1037	arg1	amoxicillin					1006:1016	amoxicillin	1006:1016	amoxicillin from 4 to 128 mg L-1	1006:1037	MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1.					
33349214	13	79	theme	Amoxycillin	1805:1815	arg1	effect					1795:1800	The synergistic effect	1779:1800	The synergistic effect of Amoxycillin in the current study	1779:1836	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	2	80	theme	chitosan	468:475	arg1	spheres					422:428	hybrid material composite spheres	396:428	hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method	396:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	10	81	theme	augmented	1464:1472	arg1	activity					1484:1491	augmented anti-MRSA activity	1464:1491	augmented anti-MRSA activity	1464:1491	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	11	82	theme	hybrid	1649:1654	arg1	formulation					1674:1684	loaded hybrid composite spheres formulation	1642:1684	loaded hybrid composite spheres formulation	1642:1684	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	0	83	theme	Amoxicillin	39:49	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.					
33349214	0	83	theme	Amoxicillin	39:49	arg1	Evaluation					25:34	Biological Evaluation	14:34	Biological Evaluation	14:34	Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.					
33349214	8	84	from	uneven	1161:1166	arg1	SEM					1202:1204	SEM	1202:1204	SEM	1202:1204	Morphology of spheres was uneven, varied, porous, and irregular in SEM, and Ag nanoparticles presence and formation was also seen in the micrograph.					
33349214	2	85	theme	one-step	501:508	arg1	method					520:525	one-step synthesis method	501:525	one-step synthesis method	501:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	3	86	theme	agar	692:695	arg1	diffusion					702:710	agar well diffusion	692:710	agar well diffusion	692:710	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	4	87	theme	RD	854:855	arg1	cell					857:860	RD cell	854:860	RD cell	854:860	The in vitro and cytotoxicity studies were conducted on the skin abrasion mouse model and MTT assay on RD cell, respectively.					
33349214	2	88	theme	Ag	446:447	arg1	nanoparticles					449:461	Ag nanoparticles	446:461	Ag nanoparticles	446:461	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	0	89	theme	Hybrid	58:63	arg1	Spheres					84:90	Hybrid Material Composite Spheres	58:90	Hybrid Material Composite Spheres	58:90	Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus.					
33349214	10	90	theme	amoxicillin	1498:1508	arg1	spheres					1534:1540	amoxicillin loaded hybrid composite spheres	1498:1540	amoxicillin loaded hybrid composite spheres (22-29 mm)	1498:1551	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	10	90	theme	amoxicillin	1498:1508	arg1	mm					1549:1550	22-29 mm	1543:1550	22-29 mm	1543:1550	The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm).					
33349214	3	91	theme	hybrid	725:730	arg1	spheres					742:748	hybrid composite spheres	725:748	hybrid composite spheres	725:748	METHODS Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques, while agar well diffusion was used for hybrid composite spheres.					
33349214	11	92	theme	spheres	1666:1672	arg1	formulation					1674:1684	loaded hybrid composite spheres formulation	1642:1684	loaded hybrid composite spheres formulation	1642:1684	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	12	93	theme	composite	1749:1757	arg1	spheres					1759:1765	hybrid composite spheres	1742:1765	hybrid composite spheres	1742:1765	The MTT assay indicated no potential cytotoxicity with hybrid composite spheres.					
33349214	13	94	theme	synergistic	1783:1793	arg1	effect					1795:1800	The synergistic effect	1779:1800	The synergistic effect of Amoxycillin in the current study	1779:1836	CONCLUSION The synergistic effect of Amoxycillin in the current study predicts a promising hybrid formulation with enhanced anti-MRSA activity.					
33349214	5	95	dep	RESULTS	877:883	arg1	isolates					889:896	All isolates	885:896	RESULTS All isolates	877:896	RESULTS All isolates showed resistance to the tested antibiotics except vancomycin.					
33349214	11	96	theme	microbial	1581:1589	arg1	mL-1					1614:1617	~6.5 log10 CFU mL-1	1599:1617	~6.5 log10 CFU mL-1	1599:1617	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	11	96	theme	microbial	1581:1589	arg1	burden					1591:1596	microbial burden	1581:1596	microbial burden (~6.5 log10 CFU mL-1)	1581:1618	A significant reduction in microbial burden (~6.5 log10 CFU mL-1) was seen in vivo with loaded hybrid composite spheres formulation.					
33349214	2	97	theme	material	403:410	arg1	spheres					422:428	hybrid material composite spheres	396:428	hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method	396:525	OBJECTIVE Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles, and chitosan, which were prepared by one-step synthesis method, and various characterizations were performed.					
33349214	1	98	theme	virulence	270:278	arg1	rate					262:265	a high rate	255:265	a high rate of virulence	255:278	BACKGROUND Incoherent use of antibiotics has led toward resistance in MRSA, becoming multidrug-resistant with a high rate of virulence in the community and hospital settings.					
34582906	4	0	theme	CuSO4	873:877	arg1	concentration					879:891	CuSO4 concentration	873:891	CuSO4 concentration	873:891	SEM clarified that the particle size of Cu2O gradually decreased and the shape changed from cubic to irregular with the increase of CuSO4 concentration.					
34582906	0	1	from	deposition	8:17	arg1	scaffolds					68:76	electrospun chitosan nanofibrous scaffolds	35:76	electrospun chitosan nanofibrous scaffolds	35:76	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	0	1	from	deposition	8:17	arg1	properties					102:111	their antimicrobial properties	82:111	their antimicrobial properties	82:111	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	4	2	theme	particle	764:771	arg1	size					773:776	the particle size	760:776	the particle size of Cu2O	760:784	SEM clarified that the particle size of Cu2O gradually decreased and the shape changed from cubic to irregular with the increase of CuSO4 concentration.					
34582906	5	3	theme	antibacterial	1024:1036	arg1	activity					1038:1045	antibacterial activity	1024:1045	antibacterial activity	1024:1045	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	1	4	theme	antimicrobial	147:159	arg1	effect					161:166	a synergistic antimicrobial effect	133:166	a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers	133:237	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	5	5	theme	nanofibrous	1168:1178	arg1	scaffolds					1180:1188	the CS nanofibrous scaffolds	1161:1188	the CS nanofibrous scaffolds	1161:1188	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	5	5	theme	nanofibrous	1168:1178	arg1	meanwhile					1191:1199	meanwhile	1191:1199	meanwhile	1191:1199	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	3	6	theme	nanofibrous	718:728	arg1	scaffolds					730:738	the CS nanofibrous scaffolds	711:738	the CS nanofibrous scaffolds	711:738	And by the measurements of XRD, XPS and FTIR-ATR, the results showed that Cu2O NPs were successfully deposited on the CS nanofibrous scaffolds.					
34582906	1	7	theme	subsequent	312:321	arg1	steps					323:327	two subsequent steps	308:327	two subsequent steps of chelation and reduction	308:354	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	1	8	theme	nanofibers	228:237	arg1	effect					161:166	a synergistic antimicrobial effect	133:166	a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers	133:237	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	0	9	theme	antimicrobial	88:100	arg1	properties					102:111	their antimicrobial properties	82:111	their antimicrobial properties	82:111	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	2	10	theme	group	449:453	arg1	coordination					427:438	the coordination	423:438	the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+	423:501	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	6	11	contain	have	1306:1309	arg2	application					1317:1327	broad application prospects	1311:1337	broad application prospects	1311:1337	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	6	11	contain	have	1306:1309	arg1	kind					1245:1248	This kind	1240:1248	This kind of nanofibrous scaffolds deposited with nano Cu2O	1240:1298	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	6	12	theme	nano	1290:1293	arg1	Cu2O					1295:1298	nano Cu2O	1290:1298	nano Cu2O	1290:1298	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	3	13	theme	XPS	629:631	arg1	measurements					608:619	the measurements	604:619	the measurements of XRD, XPS and FTIR-ATR	604:644	And by the measurements of XRD, XPS and FTIR-ATR, the results showed that Cu2O NPs were successfully deposited on the CS nanofibrous scaffolds.					
34582906	2	14	theme	Vitamin	560:566	arg1	C					568:568	Vitamin C	560:568	Vitamin C under alkaline conditions	560:594	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	4	15	theme	concentration	879:891	arg1	increase					861:868	the increase	857:868	the increase of CuSO4 concentration	857:891	SEM clarified that the particle size of Cu2O gradually decreased and the shape changed from cubic to irregular with the increase of CuSO4 concentration.					
34582906	3	16	theme	CS	715:716	arg1	scaffolds					730:738	the CS nanofibrous scaffolds	711:738	the CS nanofibrous scaffolds	711:738	And by the measurements of XRD, XPS and FTIR-ATR, the results showed that Cu2O NPs were successfully deposited on the CS nanofibrous scaffolds.					
34582906	2	17	theme	group	475:479	arg1	coordination					427:438	the coordination	423:438	the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+	423:501	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	1	18	theme	chelation	332:340	arg1	steps					323:327	two subsequent steps	308:327	two subsequent steps of chelation and reduction	308:354	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	2	19	theme	hydroxyl	466:473	arg1	group					475:479	hydroxyl group	466:479	hydroxyl group (-OH)	466:485	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	2	19	theme	hydroxyl	466:473	arg1	-OH					482:484	-OH	482:484	-OH	482:484	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	3	20	theme	XRD	624:626	arg1	measurements					608:619	the measurements	604:619	the measurements of XRD, XPS and FTIR-ATR	604:644	And by the measurements of XRD, XPS and FTIR-ATR, the results showed that Cu2O NPs were successfully deposited on the CS nanofibrous scaffolds.					
34582906	2	21	theme	nano	547:550	arg1	Cu2O					552:555	nano Cu2O	547:555	nano Cu2O	547:555	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	6	22	theme	biomaterials	1369:1380	arg1	field					1346:1350	the field	1342:1350	the field of antibacterial biomaterials	1342:1380	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	1	23	theme	cuprous	171:177	arg1	oxide					179:183	cuprous oxide	171:183	cuprous oxide nanoparticles (Cu2O NPs)	171:208	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	1	24	theme	reduction	346:354	arg1	steps					323:327	two subsequent steps	308:327	two subsequent steps of chelation and reduction	308:354	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	6	25	dep	application	1317:1327	arg1	prospects					1329:1337	prospects	1329:1337	prospects	1329:1337	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	2	26	from	coordination	427:438	arg1	CS					490:491	CS	490:491	CS with Cu2+	490:501	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	1	27	theme	oxide	179:183	arg1	nanoparticles					185:197	cuprous oxide nanoparticles	171:197	cuprous oxide nanoparticles (Cu2O NPs)	171:208	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	1	27	theme	oxide	179:183	arg1	NPs					205:207	Cu2O NPs	200:207	Cu2O NPs	200:207	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	0	28	theme	In	0:1	arg1	deposition					8:17	In situ deposition	0:17	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.	0:112	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	1	29	theme	nano	244:247	arg1	scaffolds					269:277	the nano Cu2O/CS nanofibrous scaffolds	240:277	the nano Cu2O/CS nanofibrous scaffolds	240:277	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	5	30	theme	good	1217:1220	arg1	biocompatibility					1222:1237	good biocompatibility	1217:1237	good biocompatibility	1217:1237	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	1	31	theme	nanoparticles	185:197	arg1	effect					161:166	a synergistic antimicrobial effect	133:166	a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers	133:237	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	6	32	theme	antibacterial	1355:1367	arg1	biomaterials					1369:1380	antibacterial biomaterials	1355:1380	antibacterial biomaterials	1355:1380	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	1	33	theme	Cu2O/CS	249:255	arg1	scaffolds					269:277	the nano Cu2O/CS nanofibrous scaffolds	240:277	the nano Cu2O/CS nanofibrous scaffolds	240:277	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	6	34	theme	scaffolds	1265:1273	arg1	kind					1245:1248	This kind	1240:1248	This kind of nanofibrous scaffolds deposited with nano Cu2O	1240:1298	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	2	35	theme	alkaline	576:583	arg1	conditions					585:594	alkaline conditions	576:594	alkaline conditions	576:594	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	5	36	theme	CuSO4	903:907	arg1	concentration					909:921	the CuSO4 concentration	899:921	the CuSO4 concentration of 0.02 and 0.04 mol·L-1	899:946	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	2	37	theme	CS	390:391	arg1	scaffolds					405:413	CS nanofibrous scaffolds	390:413	CS nanofibrous scaffolds	390:413	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	2	38	theme	nanofibrous	393:403	arg1	scaffolds					405:413	CS nanofibrous scaffolds	390:413	CS nanofibrous scaffolds	390:413	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	1	39	theme	nanofibrous	257:267	arg1	scaffolds					269:277	the nano Cu2O/CS nanofibrous scaffolds	240:277	the nano Cu2O/CS nanofibrous scaffolds	240:277	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	6	40	theme	nanofibrous	1253:1263	arg1	scaffolds					1265:1273	nanofibrous scaffolds	1253:1273	nanofibrous scaffolds deposited with nano Cu2O	1253:1298	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	5	41	theme	Cu2O/CS	953:959	arg1	scaffolds					973:981	the Cu2O/CS nanofibrous scaffolds	949:981	the Cu2O/CS nanofibrous scaffolds	949:981	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	1	42	theme	Cu2O	200:203	arg1	nanoparticles					185:197	cuprous oxide nanoparticles	171:197	cuprous oxide nanoparticles (Cu2O NPs)	171:208	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	1	42	theme	Cu2O	200:203	arg1	NPs					205:207	Cu2O NPs	200:207	Cu2O NPs	200:207	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	0	43	theme	Cu2O	27:30	arg1	deposition					8:17	In situ deposition	0:17	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.	0:112	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	5	44	theme	nanofibrous	961:971	arg1	scaffolds					973:981	the Cu2O/CS nanofibrous scaffolds	949:981	the Cu2O/CS nanofibrous scaffolds	949:981	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	5	45	contain	possessed	1207:1215	arg1	they					1202:1205	they	1202:1205	they	1202:1205	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	5	45	contain	possessed	1207:1215	arg2	biocompatibility					1222:1237	good biocompatibility	1217:1237	good biocompatibility	1217:1237	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	0	46	theme	nano	22:25	arg1	Cu2O					27:30	nano Cu2O	22:30	nano Cu2O	22:30	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	3	47	theme	Cu2O	671:674	arg1	NPs					676:678	Cu2O NPs	671:678	Cu2O NPs	671:678	And by the measurements of XRD, XPS and FTIR-ATR, the results showed that Cu2O NPs were successfully deposited on the CS nanofibrous scaffolds.					
34582906	2	48	theme	amino	443:447	arg1	-NH2					456:459	-NH2	456:459	-NH2	456:459	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	2	48	theme	amino	443:447	arg1	group					449:453	amino group	443:453	amino group (-NH2)	443:460	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	0	49	theme	electrospun	35:45	arg1	scaffolds					68:76	electrospun chitosan nanofibrous scaffolds	35:76	electrospun chitosan nanofibrous scaffolds	35:76	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	5	50	theme	outstanding	993:1003	arg1	hydrophilicity					1005:1018	outstanding hydrophilicity	993:1018	outstanding hydrophilicity	993:1018	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	6	51	theme	broad	1311:1315	arg1	application					1317:1327	broad application prospects	1311:1337	broad application prospects	1311:1337	This kind of nanofibrous scaffolds deposited with nano Cu2O would have broad application prospects in the field of antibacterial biomaterials.					
34582906	5	52	dep	coli	1081:1084	arg1	coli					1090:1093	E. coli	1087:1093	E. coli	1087:1093	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	4	53	dep	cubic	833:837	arg1	irregular					842:850	irregular	842:850	irregular	842:850	SEM clarified that the particle size of Cu2O gradually decreased and the shape changed from cubic to irregular with the increase of CuSO4 concentration.					
34582906	4	53	dep	cubic	833:837	arg1	to					839:840	to	839:840	to	839:840	SEM clarified that the particle size of Cu2O gradually decreased and the shape changed from cubic to irregular with the increase of CuSO4 concentration.					
34582906	4	54	theme	Cu2O	781:784	arg1	size					773:776	the particle size	760:776	the particle size of Cu2O	760:784	SEM clarified that the particle size of Cu2O gradually decreased and the shape changed from cubic to irregular with the increase of CuSO4 concentration.					
34582906	5	55	theme	0.02	926:929	arg1	concentration					909:921	the CuSO4 concentration	899:921	the CuSO4 concentration of 0.02 and 0.04 mol·L-1	899:946	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	2	56	theme	chelated	517:524	arg1	Cu2+					526:529	the chelated Cu2+	513:529	the chelated Cu2+	513:529	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	5	57	theme	0.04 mol·L-1	935:946	arg1	concentration					909:921	the CuSO4 concentration	899:921	the CuSO4 concentration of 0.02 and 0.04 mol·L-1	899:946	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	3	58	theme	FTIR-ATR	637:644	arg1	measurements					608:619	the measurements	604:619	the measurements of XRD, XPS and FTIR-ATR	604:644	And by the measurements of XRD, XPS and FTIR-ATR, the results showed that Cu2O NPs were successfully deposited on the CS nanofibrous scaffolds.					
34582906	5	59	dep	aureus	1129:1134	arg1	aureus					1140:1145	S. aureus	1137:1145	S. aureus	1137:1145	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	1	60	theme	chitosan	214:221	arg1	nanofibers					228:237	chitosan (CS) nanofibers	214:237	chitosan (CS) nanofibers	214:237	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	0	61	theme	nanofibrous	56:66	arg1	scaffolds					68:76	electrospun chitosan nanofibrous scaffolds	35:76	electrospun chitosan nanofibrous scaffolds	35:76	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	2	62	with	CS	490:491	arg1	Cu2+					498:501	Cu2+	498:501	Cu2+	498:501	The Cu2+ were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH2) and hydroxyl group (-OH) on CS with Cu2+, and then the chelated Cu2+ were reduced to nano Cu2O by Vitamin C under alkaline conditions.					
34582906	0	63	theme	chitosan	47:54	arg1	scaffolds					68:76	electrospun chitosan nanofibrous scaffolds	35:76	electrospun chitosan nanofibrous scaffolds	35:76	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	0	64	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.					
34582906	1	65	theme	synergistic	135:145	arg1	effect					161:166	a synergistic antimicrobial effect	133:166	a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers	133:237	In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (Cu2O NPs) and chitosan (CS) nanofibers, the nano Cu2O/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction.					
34582906	5	66	theme	CS	1165:1166	arg1	scaffolds					1180:1188	the CS nanofibrous scaffolds	1161:1188	the CS nanofibrous scaffolds	1161:1188	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
34582906	5	66	theme	CS	1165:1166	arg1	meanwhile					1191:1199	meanwhile	1191:1199	meanwhile	1191:1199	With the CuSO4 concentration of 0.02 and 0.04 mol·L-1, the Cu2O/CS nanofibrous scaffolds presented outstanding hydrophilicity and antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) comparing to the CS nanofibrous scaffolds, meanwhile, they possessed good biocompatibility.					
32519334	0	0	theme	sensory	64:70	arg1	properties					72:81	physical, chemical, and sensory properties	40:81	physical, chemical, and sensory properties of oat flour and banana cake	40:110	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	5	1	with	"	778:778	arg1	acceptance					800:809	good sensorial acceptance	785:809	good sensorial acceptance	785:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	4	2	theme	sensorial	666:674	arg1	acceptance					676:685	the sensorial acceptance	662:685	the sensorial acceptance of the product	662:700	The firmness of the cake increased with SB concentration, but this fact did not interfere in the sensorial acceptance of the product.					
32519334	1	3	theme	addition	193:200	arg1	effect					160:165	the effect	156:165	the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes	156:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	1	4	theme	banana	269:274	arg1	cakes					276:280	the oat flour and banana cakes	251:280	cakes	276:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	2	5	dep	high	406:409	arg1	"					428:428	"	428:428	" respectively	428:441	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	6	theme	fiber	394:398	arg1	"					399:399	source of fiber"	384:399	source of fiber"	384:399	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	1	7	theme	cakes	276:280	arg1	properties					237:246	chemical, physical, and sensory properties	205:246	chemical, physical, and sensory properties of the oat flour and banana cakes	205:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	5	8	dep	reuse	845:849	arg1	sugar					902:906	the sugar	898:906	the sugar	898:906	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	1	9	theme	chemical	205:212	arg1	properties					237:246	chemical, physical, and sensory properties	205:246	chemical, physical, and sensory properties of the oat flour and banana cakes	205:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	2	10	theme	of	391:392	arg1	"					399:399	source of fiber"	384:399	source of fiber"	384:399	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	5	11	theme	available	888:896	arg1	residue					873:879	an agro-industrial residue	854:879	an agro-industrial residue widely available	854:896	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	12	theme	high	760:763	arg1	content					771:777	high fiber content	760:777	"high fiber content" with good sensorial acceptance	759:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	2	13	from	content	420:426	arg1	high					406:409	high	406:409	high	406:409	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	14	theme	source	384:389	arg1	"					399:399	source of fiber"	384:399	source of fiber"	384:399	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	4	15	theme	cake	589:592	arg1	firmness					573:580	The firmness	569:580	The firmness of the cake	569:592	The firmness of the cake increased with SB concentration, but this fact did not interfere in the sensorial acceptance of the product.					
32519334	1	16	theme	work	135:138	arg1	objective					117:125	The objective	113:125	The objective of this work	113:138	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	0	17	theme	oat	86:88	arg1	flour					90:94	oat flour	86:94	oat flour	86:94	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	5	18	theme	alcohol	912:918	arg1	industry					920:927	alcohol industry	912:927	alcohol industry	912:927	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	19	theme	content	771:777	arg1	"					778:778	"high fiber content"	759:778	"high fiber content" with good sensorial acceptance	759:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	0	20	theme	flour	90:94	arg1	properties					72:81	physical, chemical, and sensory properties	40:81	physical, chemical, and sensory properties of oat flour and banana cake	40:110	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	2	21	theme	solids	335:340	arg1	solids					335:340	6 g/100 g solids	325:340	6 g/100 g solids (BC3 and BC6)	325:354	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	21	theme	solids	335:340	arg1	BC6					351:353	BC6	351:353	BC6	351:353	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	21	theme	solids	335:340	arg1	amount					307:312	the amount	303:312	the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively	303:441	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	21	theme	solids	335:340	arg1	g					319:319	3 g	317:319	3 g	317:319	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	21	theme	solids	335:340	arg1	BC3					343:345	BC3	343:345	BC3	343:345	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	1	22	theme	physical	215:222	arg1	properties					237:246	chemical, physical, and sensory properties	205:246	chemical, physical, and sensory properties of the oat flour and banana cakes	205:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	2	23	dep	solids	335:340	arg1	solids					335:340	6 g/100 g solids	325:340	6 g/100 g solids (BC3 and BC6)	325:354	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	23	dep	solids	335:340	arg1	BC6					351:353	BC6	351:353	BC6	351:353	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	23	dep	solids	335:340	arg1	BC3					343:345	BC3	343:345	BC3	343:345	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	5	24	theme	banana	735:740	arg1	cakes					742:746	oat flour and banana cakes	721:746	cakes	742:746	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	25	contain	containing	748:757	arg2	"					778:778	"high fiber content"	759:778	"high fiber content" with good sensorial acceptance	759:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	25	contain	containing	748:757	arg1	flour					725:729	oat flour and banana cakes	721:746	flour	725:729	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	26	theme	agro-industrial	857:871	arg1	residue					873:879	an agro-industrial residue	854:879	an agro-industrial residue widely available	854:896	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	2	27	theme	g/100 g	327:333	arg1	solids					335:340	6 g/100 g solids	325:340	6 g/100 g solids (BC3 and BC6)	325:354	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	27	theme	g/100 g	327:333	arg1	BC6					351:353	BC6	351:353	BC6	351:353	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	27	theme	g/100 g	327:333	arg1	BC3					343:345	BC3	343:345	BC3	343:345	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	0	28	theme	banana	100:105	arg1	cake					107:110	banana cake	100:110	banana cake	100:110	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	5	29	theme	cakes	742:746	arg1	possible					815:822	possible	815:822	possible	815:822	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	29	theme	cakes	742:746	arg1	production					707:716	The production	703:716	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance	703:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	0	30	theme	sugarcane	10:18	arg1	Effect					0:5	Effect	0:5	Effect of sugarcane	0:18	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	5	31	theme	fiber	765:769	arg1	content					771:777	high fiber content	760:777	"high fiber content" with good sensorial acceptance	759:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	2	32	theme	g	319:319	arg1	solids					335:340	6 g/100 g solids	325:340	6 g/100 g solids (BC3 and BC6)	325:354	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	32	theme	g	319:319	arg1	BC6					351:353	BC6	351:353	BC6	351:353	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	32	theme	g	319:319	arg1	amount					307:312	the amount	303:312	the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively	303:441	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	32	theme	g	319:319	arg1	g					319:319	3 g	317:319	3 g	317:319	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	2	32	theme	g	319:319	arg1	BC3					343:345	BC3	343:345	BC3	343:345	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	1	33	theme	sensory	229:235	arg1	properties					237:246	chemical, physical, and sensory properties	205:246	chemical, physical, and sensory properties of the oat flour and banana cakes	205:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	3	34	theme	chemical	481:488	arg1	composition					490:500	the chemical composition	477:500	the chemical composition of the cakes	477:513	The addition of SB did not alter the chemical composition of the cakes except the dietary fiber which was higher in the BC6.					
32519334	5	35	theme	sensorial	790:798	arg1	acceptance					800:809	good sensorial acceptance	785:809	good sensorial acceptance	785:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	0	36	theme	cake	107:110	arg1	properties					72:81	physical, chemical, and sensory properties	40:81	physical, chemical, and sensory properties of oat flour and banana cake	40:110	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	5	37	theme	residue	873:879	arg1	reuse					845:849	the reuse	841:849	the reuse of an agro-industrial residue widely available	841:896	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	37	theme	residue	873:879	arg1	industry					920:927	alcohol industry	912:927	alcohol industry	912:927	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	1	38	theme	sugarcane	170:178	arg1	SB					189:190	SB	189:190	SB	189:190	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	1	38	theme	sugarcane	170:178	arg1	bagasse					180:186	sugarcane bagasse	170:186	sugarcane bagasse (SB) addition	170:200	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	4	39	theme	product	694:700	arg1	acceptance					676:685	the sensorial acceptance	662:685	the sensorial acceptance of the product	662:700	The firmness of the cake increased with SB concentration, but this fact did not interfere in the sensorial acceptance of the product.					
32519334	0	40	theme	physical	40:47	arg1	properties					72:81	physical, chemical, and sensory properties	40:81	physical, chemical, and sensory properties of oat flour and banana cake	40:110	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	3	41	theme	dietary	526:532	arg1	fiber					534:538	the dietary fiber	522:538	the dietary fiber which was higher in the BC6	522:566	The addition of SB did not alter the chemical composition of the cakes except the dietary fiber which was higher in the BC6.					
32519334	3	41	theme	dietary	526:532	arg1	higher					550:555	higher	550:555	higher	550:555	The addition of SB did not alter the chemical composition of the cakes except the dietary fiber which was higher in the BC6.					
32519334	1	42	theme	oat	255:257	arg1	flour					259:263	the oat flour and banana cakes	251:280	flour	259:263	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	2	43	theme	fiber	414:418	arg1	content					420:426	fiber content	414:426	fiber content	414:426	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	3	44	theme	cakes	509:513	arg1	composition					490:500	the chemical composition	477:500	the chemical composition of the cakes	477:513	The addition of SB did not alter the chemical composition of the cakes except the dietary fiber which was higher in the BC6.					
32519334	3	45	theme	SB	460:461	arg1	addition					448:455	The addition	444:455	The addition of SB	444:461	The addition of SB did not alter the chemical composition of the cakes except the dietary fiber which was higher in the BC6.					
32519334	1	46	from	effect	160:165	arg1	properties					237:246	chemical, physical, and sensory properties	205:246	chemical, physical, and sensory properties of the oat flour and banana cakes	205:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	5	47	theme	oat	721:723	arg1	flour					725:729	oat flour and banana cakes	721:746	flour	725:729	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	1	48	theme	bagasse	180:186	arg1	addition					193:200	sugarcane bagasse (SB) addition	170:200	sugarcane bagasse (SB) addition	170:200	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	5	49	theme	flour	725:729	arg1	possible					815:822	possible	815:822	possible	815:822	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	49	theme	flour	725:729	arg1	production					707:716	The production	703:716	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance	703:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	5	50	theme	good	785:788	arg1	acceptance					800:809	good sensorial acceptance	785:809	good sensorial acceptance	785:809	The production of oat flour and banana cakes containing "high fiber content" with good sensorial acceptance was possible and demonstrated the reuse of an agro-industrial residue widely available the sugar and alcohol industry.					
32519334	2	51	from	high	406:409	arg1	content					420:426	fiber content	414:426	fiber content	414:426	The SB was added in the amount of 3 g and 6 g/100 g solids (BC3 and BC6) to obtain cakes considered "source of fiber" and "high in fiber content," respectively.					
32519334	0	52	theme	chemical	50:57	arg1	properties					72:81	physical, chemical, and sensory properties	40:81	physical, chemical, and sensory properties of oat flour and banana cake	40:110	Effect of sugarcane bagasse addition on physical, chemical, and sensory properties of oat flour and banana cake.					
32519334	1	53	theme	flour	259:263	arg1	properties					237:246	chemical, physical, and sensory properties	205:246	chemical, physical, and sensory properties of the oat flour and banana cakes	205:280	The objective of this work was to evaluate the effect of sugarcane bagasse (SB) addition on chemical, physical, and sensory properties of the oat flour and banana cakes.					
32519334	4	54	theme	SB	609:610	arg1	concentration					612:624	SB concentration	609:624	SB concentration	609:624	The firmness of the cake increased with SB concentration, but this fact did not interfere in the sensorial acceptance of the product.					
34681325	4	0	theme	structural	669:678	arg1	characteristics					680:694	structural characteristics	669:694	structural characteristics	669:694	The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different.					
34681325	6	1	theme	in	955:956	arg1	capabilities					977:988	in vitro hypoglycemic capabilities	955:988	the strongest in vitro hypoglycemic capabilities	941:988	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	6	2	dep	in	955:956	arg1	vitro					958:962	vitro	958:962	vitro	958:962	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	7	3	theme	acid	1073:1076	arg1	content					1078:1084	uronic acid content	1066:1084	uronic acid content	1066:1084	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	6	4	theme	strongest	945:953	arg1	capabilities					977:988	in vitro hypoglycemic capabilities	955:988	the strongest in vitro hypoglycemic capabilities	941:988	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	4	5	theme	DTPSs	734:738	arg1	properties					712:721	biological properties	701:721	biological properties	701:721	The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different.					
34681325	4	5	theme	DTPSs	734:738	arg1	different					745:753	different	745:753	different	745:753	The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different.					
34681325	4	5	theme	DTPSs	734:738	arg1	compositions					655:666	the compositions	651:666	the compositions	651:666	The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different.					
34681325	4	5	theme	DTPSs	734:738	arg1	characteristics					680:694	structural characteristics	669:694	structural characteristics	669:694	The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different.					
34681325	1	6	dep	structure	132:140	arg1	The					128:130	The	128:130	The	128:130	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	0	7	theme	Hypoglycemic	103:114	arg1	Properties					116:125	Hypoglycemic Properties	103:125	Hypoglycemic Properties	103:125	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	7	8	theme	hypoglycemic	1138:1149	arg1	activities					1151:1160	their antioxidant and hypoglycemic activities	1116:1160	their antioxidant and hypoglycemic activities	1116:1160	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	8	9	theme	functional	1240:1249	arg1	foods					1251:1255	functional foods	1240:1255	functional foods	1240:1255	Our results indicated that DTPS-4 and DTPS-6 could be further developed into functional foods or additives, respectively.					
34681325	1	10	theme	tea	171:173	arg1	polysaccharides					175:189	tea polysaccharides	171:189	tea polysaccharides	171:189	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	7	11	theme	uronic	1066:1071	arg1	content					1078:1084	uronic acid content	1066:1084	uronic acid content	1066:1084	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	1	12	theme	polysaccharides	175:189	arg1	structure					132:140	structure	132:140	structure	132:140	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	1	12	theme	polysaccharides	175:189	arg1	activity					159:166	hypoglycemic activity	146:166	hypoglycemic activity	146:166	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	7	13	theme	molecular	1045:1053	arg1	related					1105:1111	related	1105:1111	related	1105:1111	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	7	13	theme	molecular	1045:1053	arg1	weight					1055:1060	the molecular weight	1041:1060	the molecular weight	1041:1060	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	7	14	theme	correlation	1006:1016	arg1	analysis					1018:1025	a correlation analysis	1004:1025	a correlation analysis	1004:1025	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	3	15	theme	polysaccharide	421:434	arg1	fractions					436:444	Six polysaccharide fractions	417:444	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6)	417:504	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	15	theme	polysaccharide	421:434	arg1	DTPS-6					498:503	DTPS-6	498:503	DTPS-6	498:503	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	15	theme	polysaccharide	421:434	arg1	DTPS-3					470:475	DTPS-3	470:475	DTPS-3	470:475	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	15	theme	polysaccharide	421:434	arg1	DTPS-4					478:483	DTPS-4	478:483	DTPS-4	478:483	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	15	theme	polysaccharide	421:434	arg1	DTPS-1					454:459	DTPS-1	454:459	DTPS-1	454:459	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	15	theme	polysaccharide	421:434	arg1	DTPS-2					462:467	DTPS-2	462:467	DTPS-2	462:467	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	15	theme	polysaccharide	421:434	arg1	DTPS-5					486:491	DTPS-5	486:491	DTPS-5	486:491	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	1	16	theme	hypoglycemic	277:288	arg1	activity					290:297	hypoglycemic activity	277:297	hypoglycemic activity	277:297	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	6	17	theme	in	891:892	arg1	capabilities					912:923	the optimum in vitro antioxidant capabilities	879:923	the optimum in vitro antioxidant capabilities	879:923	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	5	18	theme	stable	836:841	arg1	structure					843:851	a more stable structure	829:851	a more stable structure	829:851	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	5	19	theme	looser	787:792	arg1	morphology					794:803	looser morphology	787:803	looser morphology	787:803	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	3	20	attach	isolated	511:518	arg2	DTPS-3					470:475	DTPS-3	470:475	DTPS-3	470:475	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg2	DTPS-1					454:459	DTPS-1	454:459	DTPS-1	454:459	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg1	CDTPS					525:529	CDTPS	525:529	CDTPS	525:529	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg2	DTPS-4					478:483	DTPS-4	478:483	DTPS-4	478:483	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg2	DTPS-2					462:467	DTPS-2	462:467	DTPS-2	462:467	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg2	DTPS-5					486:491	DTPS-5	486:491	DTPS-5	486:491	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg2	DTPS-6					498:503	DTPS-6	498:503	DTPS-6	498:503	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	20	attach	isolated	511:518	arg2	fractions					436:444	Six polysaccharide fractions	417:444	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6)	417:504	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	0	21	theme	Polysaccharides	16:30	arg1	Valorization					0:11	Valorization	0:11	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.	0:126	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	5	22	theme	faster	806:811	arg1	solubility					813:822	faster solubility	806:822	faster solubility	806:822	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	0	23	theme	Dark	46:49	arg1	Tea					51:53	Dark Tea	46:53	Dark Tea	46:53	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	1	24	theme	dark	302:305	arg1	polysaccharides					311:325	dark tea polysaccharides	302:325	dark tea polysaccharides	302:325	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	6	25	dep	in	891:892	arg1	vitro					894:898	vitro	894:898	vitro	894:898	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	3	26	theme	structural	559:568	arg1	properties					586:595	their physicochemical, structural, and biological properties	536:595	their physicochemical, structural, and biological properties	536:595	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	2	27	theme	Fuzhuan	399:405	arg1	tea					412:414	Fuzhuan dark tea	399:414	Fuzhuan dark tea	399:414	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	1	28	theme	tea	307:309	arg1	polysaccharides					311:325	dark tea polysaccharides	302:325	dark tea polysaccharides	302:325	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	7	29	theme	antioxidant	1122:1132	arg1	activities					1151:1160	their antioxidant and hypoglycemic activities	1116:1160	their antioxidant and hypoglycemic activities	1116:1160	In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities.					
34681325	5	30	contain	had	783:785	arg2	morphology					794:803	looser morphology	787:803	looser morphology	787:803	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	5	30	contain	had	783:785	arg2	structure					843:851	a more stable structure	829:851	a more stable structure	829:851	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	5	30	contain	had	783:785	arg1	DTPS-6					776:781	DTPS-6	776:781	DTPS-6	776:781	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	5	30	contain	had	783:785	arg1	DTPS-4					765:770	DTPS-4	765:770	DTPS-4	765:770	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	5	30	contain	had	783:785	arg1	Therein					756:762	Therein	756:762	Therein	756:762	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	5	30	contain	had	783:785	arg2	solubility					813:822	faster solubility	806:822	faster solubility	806:822	Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure.					
34681325	3	31	dep	fractions	436:444	arg1	fractions					436:444	Six polysaccharide fractions	417:444	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6)	417:504	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	31	dep	fractions	436:444	arg1	DTPS-6					498:503	DTPS-6	498:503	DTPS-6	498:503	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	31	dep	fractions	436:444	arg1	DTPS-3					470:475	DTPS-3	470:475	DTPS-3	470:475	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	31	dep	fractions	436:444	arg1	DTPS-4					478:483	DTPS-4	478:483	DTPS-4	478:483	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	31	dep	fractions	436:444	arg1	DTPS-1					454:459	DTPS-1	454:459	DTPS-1	454:459	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	31	dep	fractions	436:444	arg1	DTPS-2					462:467	DTPS-2	462:467	DTPS-2	462:467	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	3	31	dep	fractions	436:444	arg1	DTPS-5					486:491	DTPS-5	486:491	DTPS-5	486:491	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	2	32	theme	dark	407:410	arg1	tea					412:414	Fuzhuan dark tea	399:414	Fuzhuan dark tea	399:414	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	1	33	theme	polysaccharides	311:325	arg1	characterization					256:271	characterization	256:271	characterization	256:271	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	1	33	theme	polysaccharides	311:325	arg1	activity					290:297	hypoglycemic activity	277:297	hypoglycemic activity	277:297	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	3	34	theme	physicochemical	542:556	arg1	properties					586:595	their physicochemical, structural, and biological properties	536:595	their physicochemical, structural, and biological properties	536:595	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	1	35	from	reports	241:247	arg1	characterization					256:271	characterization	256:271	characterization	256:271	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	1	35	from	reports	241:247	arg1	activity					290:297	hypoglycemic activity	277:297	hypoglycemic activity	277:297	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	1	36	dep	characterization	256:271	arg1	the					252:254	the	252:254	the	252:254	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	6	37	contain	had	937:939	arg2	capabilities					977:988	in vitro hypoglycemic capabilities	955:988	the strongest in vitro hypoglycemic capabilities	941:988	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	6	37	contain	had	937:939	arg1	DTPS-6					930:935	DTPS-6	930:935	DTPS-6	930:935	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	2	38	theme	tea	343:345	arg1	CDTPS					363:367	CDTPS	363:367	CDTPS	363:367	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	2	38	theme	tea	343:345	arg1	polysaccharide					347:360	The crude dark tea polysaccharide	328:360	The crude dark tea polysaccharide (CDTPS)	328:368	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	3	39	theme	biological	575:584	arg1	properties					586:595	their physicochemical, structural, and biological properties	536:595	their physicochemical, structural, and biological properties	536:595	Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed.					
34681325	2	40	theme	dark	338:341	arg1	CDTPS					363:367	CDTPS	363:367	CDTPS	363:367	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	2	40	theme	dark	338:341	arg1	polysaccharide					347:360	The crude dark tea polysaccharide	328:360	The crude dark tea polysaccharide (CDTPS)	328:368	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	2	41	theme	crude	332:336	arg1	CDTPS					363:367	CDTPS	363:367	CDTPS	363:367	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	2	41	theme	crude	332:336	arg1	polysaccharide					347:360	The crude dark tea polysaccharide	328:360	The crude dark tea polysaccharide (CDTPS)	328:368	The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea.					
34681325	4	42	theme	biological	701:710	arg1	properties					712:721	biological properties	701:721	biological properties	701:721	The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different.					
34681325	6	43	contain	had	875:877	arg2	capabilities					912:923	the optimum in vitro antioxidant capabilities	879:923	the optimum in vitro antioxidant capabilities	879:923	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	6	43	contain	had	875:877	arg1	DTPS-4					868:873	DTPS-4	868:873	DTPS-4	868:873	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	1	44	theme	few	237:239	arg1	reports					241:247	few reports	237:247	few reports on the characterization and hypoglycemic activity of dark tea polysaccharides	237:325	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	6	45	theme	optimum	883:889	arg1	capabilities					912:923	the optimum in vitro antioxidant capabilities	879:923	the optimum in vitro antioxidant capabilities	879:923	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	0	46	dep	Valorization	0:11	arg1	Physicochemical					69:83	Physicochemical	69:83	Physicochemical	69:83	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	0	46	dep	Valorization	0:11	arg1	Properties					116:125	Hypoglycemic Properties	103:125	Hypoglycemic Properties	103:125	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	0	46	dep	Valorization	0:11	arg1	Preparation					56:66	Preparation	56:66	Preparation	56:66	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	0	46	dep	Valorization	0:11	arg1	Antioxidant					86:96	Antioxidant	86:96	Antioxidant	86:96	Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.					
34681325	1	47	theme	hypoglycemic	146:157	arg1	activity					159:166	hypoglycemic activity	146:166	hypoglycemic activity	146:166	The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides.					
34681325	6	48	theme	antioxidant	900:910	arg1	capabilities					912:923	the optimum in vitro antioxidant capabilities	879:923	the optimum in vitro antioxidant capabilities	879:923	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
34681325	6	49	theme	hypoglycemic	964:975	arg1	capabilities					977:988	in vitro hypoglycemic capabilities	955:988	the strongest in vitro hypoglycemic capabilities	941:988	Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities.					
33937931	6	0	with	solution	1087:1094	arg1	concentrations					1154:1167	different concentrations	1144:1167	different concentrations of glutaraldehyde	1144:1185	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	9	1	theme	β-galactosidase	1889:1903	arg1	Immobilization					1871:1884	operational costs.Key Points• Immobilization	1841:1884	operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1841:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	8	2	theme	enzyme	1619:1624	arg1	cycles					1605:1610	cycles	1605:1610	cycles of the enzyme immobilized	1605:1636	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	5	3	theme	central	662:668	arg1	design					691:696	A central composite rotational design	660:696	A central composite rotational design (CCRD)	660:703	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	3	theme	central	662:668	arg1	CCRD					699:702	CCRD	699:702	CCRD	699:702	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	3	4	theme	continuous	532:541	arg1	processes					543:551	continuous processes	532:551	continuous processes	532:551	Immobilization is a viable strategy for enzyme retention inside a reactor, allowing its reuse and application in continuous processes.					
33937931	7	5	theme	immobilized	1393:1403	arg1	enzyme					1405:1410	the immobilized enzyme	1389:1410	the immobilized enzyme	1389:1410	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	9	6	theme	reactor•	1942:1949	arg1	Influence					1951:1959	batch reactor• Influence	1936:1959	batch reactor• Influence of buffer pH and ionic concentration	1936:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	5	7	theme	ionic	838:842	arg1	strength					844:851	ionic strength	838:851	ionic strength	838:851	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	9	8	theme	pH	1971:1972	arg1	Influence					1951:1959	batch reactor• Influence	1936:1959	batch reactor• Influence of buffer pH and ionic concentration	1936:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	4	9	theme	exchange	644:651	arg1	resin					653:657	ion exchange resin	640:657	ion exchange resin	640:657	Here, we studied the immobilization of β-galactosidase from Bacillus licheniformis in ion exchange resin.					
33937931	9	10	from	Influence	1951:1959	arg1	Immobilization					1871:1884	operational costs.Key Points• Immobilization	1841:1884	operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1841:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	11	theme	ionic	1978:1982	arg1	concentration					1984:1996	ionic concentration	1978:1996	ionic concentration	1978:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	5	12	theme	rotational	680:689	arg1	design					691:696	A central composite rotational design	660:696	A central composite rotational design (CCRD)	660:703	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	12	theme	rotational	680:689	arg1	CCRD					699:702	CCRD	699:702	CCRD	699:702	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	1	13	theme	food	105:108	arg1	industry					110:117	The food industry	101:117	The food industry	101:117	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	5	14	from	process	749:755	arg1	relation					760:767	relation	760:767	relation to three immobilization solution variables	760:810	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	1	15	theme	wide	135:138	arg1	range					140:144	a wide range	133:144	a wide range of products with reduced lactose to allow people with intolerance to consume dairy products	133:236	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	9	16	from	licheniformis	1919:1931	arg1	β-galactosidase					1889:1903	β-galactosidase	1889:1903	β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1889:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	16	from	licheniformis	1919:1931	arg1	Immobilization					1871:1884	operational costs.Key Points• Immobilization	1841:1884	operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1841:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	17	theme	enzyme	2010:2015	arg1	activity					2017:2024	enzyme activity	2010:2024	enzyme activity	2010:2024	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	7	18	theme	stabilization	1192:1204	arg1	step					1206:1209	The stabilization step	1188:1209	The stabilization step	1188:1209	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	8	19	theme	enzyme	1489:1494	arg1	activity					1461:1468	the initial activity	1449:1468	the initial activity of the immobilized enzyme	1449:1494	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	3	20	from	application	517:527	arg1	processes					543:551	continuous processes	532:551	continuous processes	532:551	Immobilization is a viable strategy for enzyme retention inside a reactor, allowing its reuse and application in continuous processes.					
33937931	0	21	theme	dairy	85:89	arg1	industry					91:98	the dairy industry	81:98	the dairy industry	81:98	Immobilization of β-galactosidase from Bacillus licheniformis for application in the dairy industry.					
33937931	9	22	dep	allows	1797:1802	arg1	offered					2002:2008	offered	2002:2008	offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability	2002:2096	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	22	dep	allows	1797:1802	arg1	decreasing					1830:1839	decreasing	1830:1839	decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1830:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	23	theme	dairy	1775:1779	arg1	products					1781:1788	lactose-free dairy products	1762:1788	lactose-free dairy products	1762:1788	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	8	24	theme	higher	1497:1502	arg1	L-1					1534:1536	3.5 g L-1	1528:1536	3.5 g L-1	1528:1536	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	8	24	theme	higher	1497:1502	arg1	concentrations					1512:1525	higher reagent concentrations	1497:1525	higher reagent concentrations (3.5 g L-1)	1497:1537	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	5	25	theme	mM	947:948	arg1	strength					956:963	40 mM ionic strength	944:963	40 mM ionic strength	944:963	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	9	26	theme	glutaraldehyde	2058:2071	arg1	Influence					2045:2053	immobilization• Influence	2029:2053	immobilization• Influence of glutaraldehyde on operational stability	2029:2096	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	5	27	theme	immobilization	734:747	arg1	process					749:755	the immobilization process	730:755	the immobilization process in relation to three immobilization solution variables	730:810	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	9	28	theme	operational	2076:2086	arg1	stability					2088:2096	operational stability	2076:2096	operational stability	2076:2096	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	5	29	theme	offered	813:819	arg1	activity					828:835	offered enzyme activity	813:835	offered enzyme activity	813:835	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	7	30	from	influence	1360:1368	arg1	activity					1377:1384	the activity	1373:1384	the activity of the immobilized enzyme	1373:1410	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	6	31	theme	additional	1030:1039	arg1	stabilization					1041:1053	additional stabilization	1030:1053	additional stabilization	1030:1053	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	6	32	theme	different	1144:1152	arg1	concentrations					1154:1167	different concentrations	1144:1167	different concentrations of glutaraldehyde	1144:1185	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	0	33	theme	β-galactosidase	18:32	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of β-galactosidase from Bacillus licheniformis for application in the dairy industry.	0:99	Immobilization of β-galactosidase from Bacillus licheniformis for application in the dairy industry.					
33937931	3	34	theme	viable	439:444	arg1	strategy					446:453	a viable strategy	437:453	a viable strategy for enzyme retention inside a reactor	437:491	Immobilization is a viable strategy for enzyme retention inside a reactor, allowing its reuse and application in continuous processes.					
33937931	3	34	theme	viable	439:444	arg1	Immobilization					419:432	Immobilization	419:432	Immobilization	419:432	Immobilization is a viable strategy for enzyme retention inside a reactor, allowing its reuse and application in continuous processes.					
33937931	5	35	theme	immobilization	778:791	arg1	variables					802:810	three immobilization solution variables	772:810	three immobilization solution variables	772:810	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	7	36	theme	immobilized	1252:1262	arg1	enzyme					1264:1269	the immobilized enzyme	1248:1269	the immobilized enzyme	1248:1269	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	9	37	theme	operational	1841:1851	arg1	Immobilization					1871:1884	operational costs.Key Points• Immobilization	1841:1884	operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1841:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	8	38	theme	immobilized	1626:1636	arg1	enzyme					1619:1624	the enzyme immobilized	1615:1636	the enzyme immobilized	1615:1636	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	9	39	theme	Points•	1863:1869	arg1	Immobilization					1871:1884	operational costs.Key Points• Immobilization	1841:1884	operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1841:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	7	40	with	reticulation	1280:1291	arg1	concentrations					1308:1321	different concentrations	1298:1321	different concentrations of glutaraldehyde	1298:1339	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	9	41	from	Influence	2045:2053	arg1	stability					2088:2096	operational stability	2076:2096	operational stability	2076:2096	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	8	42	theme	cycles	1605:1610	arg1	number					1595:1600	the number	1591:1600	the number of cycles of the enzyme immobilized	1591:1636	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	2	43	theme	food	298:301	arg1	industries					330:339	the food, pharma, and biotechnology industries	294:339	the food, pharma, and biotechnology industries	294:339	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	2	44	contain	has	264:266	arg1	β-galactosidase					248:262	β-galactosidase	248:262	β-galactosidase	248:262	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	2	44	contain	has	264:266	arg2	applications					278:289	extensive applications	268:289	extensive applications	268:289	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	3	45	from	reuse	507:511	arg1	processes					543:551	continuous processes	532:551	continuous processes	532:551	Immobilization is a viable strategy for enzyme retention inside a reactor, allowing its reuse and application in continuous processes.					
33937931	1	46	theme	reduced	163:169	arg1	lactose					171:177	reduced lactose	163:177	reduced lactose to allow people with intolerance to consume dairy products	163:236	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	9	47	from	β-galactosidase	1889:1903	arg1	Influence					1951:1959	batch reactor• Influence	1936:1959	batch reactor• Influence of buffer pH and ionic concentration	1936:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	48	theme	batch	1936:1940	arg1	Influence					1951:1959	batch reactor• Influence	1936:1959	batch reactor• Influence of buffer pH and ionic concentration	1936:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	49	from	licheniformis	1673:1685	arg1	β-galactosidase					1643:1657	The β-galactosidase	1639:1657	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568	1639:1713	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	49	from	licheniformis	1673:1685	arg1	technique					1730:1738	a promising technique	1718:1738	a promising technique to produce reduced	1718:1757	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	7	50	theme	enzyme	1405:1410	arg1	activity					1377:1384	the activity	1373:1384	the activity of the immobilized enzyme	1373:1410	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	2	51	theme	pharma	304:309	arg1	industries					330:339	the food, pharma, and biotechnology industries	294:339	the food, pharma, and biotechnology industries	294:339	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	9	52	from	Immobilization	1871:1884	arg1	licheniformis					1919:1931	Bacillus licheniformis	1910:1931	Bacillus licheniformis	1910:1931	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	52	from	Immobilization	1871:1884	arg1	Influence					1951:1959	batch reactor• Influence	1936:1959	batch reactor• Influence of buffer pH and ionic concentration	1936:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	6	53	with	crosslinking	1126:1137	arg1	concentrations					1154:1167	different concentrations	1144:1167	different concentrations of glutaraldehyde	1144:1185	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	9	54	theme	buffer	1964:1969	arg1	pH					1971:1972	buffer pH	1964:1972	buffer pH	1964:1972	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	55	dep	produce	1743:1749	arg1	reduced					1751:1757	reduced	1751:1757	to produce reduced	1740:1757	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	8	56	theme	initial	1453:1459	arg1	activity					1461:1468	the initial activity	1449:1468	the initial activity of the immobilized enzyme	1449:1494	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	4	57	theme	ion	640:642	arg1	resin					653:657	ion exchange resin	640:657	ion exchange resin	640:657	Here, we studied the immobilization of β-galactosidase from Bacillus licheniformis in ion exchange resin.					
33937931	5	58	theme	composite	670:678	arg1	design					691:696	A central composite rotational design	660:696	A central composite rotational design (CCRD)	660:703	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	58	theme	composite	670:678	arg1	CCRD					699:702	CCRD	699:702	CCRD	699:702	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	9	59	theme	promising	1720:1728	arg1	β-galactosidase					1643:1657	The β-galactosidase	1639:1657	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568	1639:1713	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	59	theme	promising	1720:1728	arg1	technique					1730:1738	a promising technique	1718:1738	a promising technique to produce reduced	1718:1757	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	6	60	theme	glutaraldehyde	1172:1185	arg1	concentrations					1154:1167	different concentrations	1144:1167	different concentrations of glutaraldehyde	1144:1185	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	9	61	theme	concentration	1984:1996	arg1	Influence					1951:1959	batch reactor• Influence	1936:1959	batch reactor• Influence of buffer pH and ionic concentration	1936:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	2	62	theme	extensive	268:276	arg1	applications					278:289	extensive applications	268:289	extensive applications	268:289	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	5	63	theme	enzyme	918:923	arg1	activity					925:932	enzyme activity	918:932	enzyme activity of 953 U	918:941	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	8	64	theme	immobilized	1477:1487	arg1	enzyme					1489:1494	the immobilized enzyme	1473:1494	the immobilized enzyme	1473:1494	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	7	65	theme	significant	1348:1358	arg1	influence					1360:1368	significant influence	1348:1368	significant influence on the activity of the immobilized enzyme	1348:1410	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	7	66	theme	glutaraldehyde	1326:1339	arg1	concentrations					1308:1321	different concentrations	1298:1321	different concentrations of glutaraldehyde	1298:1339	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	1	67	with	products	149:156	arg1	lactose					171:177	reduced lactose	163:177	reduced lactose to allow people with intolerance to consume dairy products	163:236	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	1	68	theme	products	149:156	arg1	range					140:144	a wide range	133:144	a wide range of products with reduced lactose to allow people with intolerance to consume dairy products	133:236	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	9	69	theme	lactose-free	1762:1773	arg1	products					1781:1788	lactose-free dairy products	1762:1788	lactose-free dairy products	1762:1788	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	8	70	theme	reagent	1504:1510	arg1	L-1					1534:1536	3.5 g L-1	1528:1536	3.5 g L-1	1528:1536	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	8	70	theme	reagent	1504:1510	arg1	concentrations					1512:1525	higher reagent concentrations	1497:1525	higher reagent concentrations (3.5 g L-1)	1497:1537	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	9	71	theme	immobilization•	2029:2043	arg1	Influence					2045:2053	immobilization• Influence	2029:2053	immobilization• Influence of glutaraldehyde on operational stability	2029:2096	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	9	72	dep	technique	1730:1738	arg1	produce					1743:1749	produce	1743:1749	to produce reduced	1740:1757	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	5	73	theme	40	944:945	arg1	mM					947:948	mM	947:948	mM	947:948	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	74	theme	ionic	950:954	arg1	strength					956:963	40 mM ionic strength	944:963	40 mM ionic strength	944:963	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	7	75	theme	different	1298:1306	arg1	concentrations					1308:1321	different concentrations	1298:1321	different concentrations of glutaraldehyde	1298:1339	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	0	76	from	licheniformis	48:60	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of β-galactosidase from Bacillus licheniformis for application in the dairy industry.	0:99	Immobilization of β-galactosidase from Bacillus licheniformis for application in the dairy industry.					
33937931	6	77	from	°C	1109:1110	arg1	solution					1087:1094	a buffer solution	1078:1094	a buffer solution pH 9.0 at 25 °C for 24 h	1078:1119	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	6	77	from	°C	1109:1110	arg1	crosslinking					1126:1137	crosslinking	1126:1137	crosslinking with different concentrations of glutaraldehyde	1126:1185	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	5	78	theme	enzyme	821:826	arg1	activity					828:835	offered enzyme activity	813:835	offered enzyme activity	813:835	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	79	theme	U	941:941	arg1	pH					970:971	pH 4.0	970:975	pH 4.0	970:975	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	79	theme	U	941:941	arg1	strength					956:963	40 mM ionic strength	944:963	40 mM ionic strength	944:963	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	5	79	theme	U	941:941	arg1	activity					925:932	enzyme activity	918:932	enzyme activity of 953 U	918:941	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	3	80	theme	enzyme	459:464	arg1	retention					466:474	enzyme retention	459:474	enzyme retention inside a reactor	459:491	Immobilization is a viable strategy for enzyme retention inside a reactor, allowing its reuse and application in continuous processes.					
33937931	1	81	with	people	188:193	arg1	intolerance					200:210	intolerance	200:210	intolerance	200:210	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	7	82	theme	enzyme	1264:1269	arg1	activity					1236:1243	the activity	1232:1243	the activity of the immobilized enzyme	1232:1269	The stabilization step drastically impacted the activity of the immobilized enzyme, and the reticulation with different concentrations of glutaraldehyde showed significant influence on the activity of the immobilized enzyme.					
33937931	9	83	theme	biocatalyst	1817:1827	arg1	reuse					1804:1808	reuse	1804:1808	reuse of the biocatalyst	1804:1827	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	5	84	theme	solution	793:800	arg1	variables					802:810	three immobilization solution variables	772:810	three immobilization solution variables	772:810	A central composite rotational design (CCRD) was proposed to evaluate the immobilization process in relation to three immobilization solution variables: offered enzyme activity, ionic strength, and pH. The conditions that maximized the response were offered enzyme activity of 953 U, 40 mM ionic strength, and pH 4.0.					
33937931	8	85	theme	g	1532:1532	arg1	L-1					1534:1536	3.5 g L-1	1528:1536	3.5 g L-1	1528:1536	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	8	85	theme	g	1532:1532	arg1	concentrations					1512:1525	higher reagent concentrations	1497:1525	higher reagent concentrations (3.5 g L-1)	1497:1537	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	8	86	theme	related	1580:1586	arg1	stability					1570:1578	stability	1570:1578	stability related to the number of cycles of the enzyme immobilized	1570:1636	In spite of substantially affecting the initial activity of the immobilized enzyme, higher reagent concentrations (3.5 g L-1) were effective for maintaining stability related to the number of cycles of the enzyme immobilized.					
33937931	9	87	theme	costs.Key	1853:1861	arg1	Immobilization					1871:1884	operational costs.Key Points• Immobilization	1841:1884	operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration	1841:1996	The β-galactosidase from Bacillus licheniformis immobilized in Duolite A568 is a promising technique to produce reduced or lactose-free dairy products, as it allows reuse of the biocatalyst, decreasing operational costs.Key Points• Immobilization of β-galactosidase from Bacillus licheniformis in batch reactor• Influence of buffer pH and ionic concentration and offered enzyme activity on immobilization• Influence of glutaraldehyde on operational stability.					
33937931	4	88	theme	β-galactosidase	593:607	arg1	immobilization					575:588	the immobilization	571:588	the immobilization of β-galactosidase	571:607	Here, we studied the immobilization of β-galactosidase from Bacillus licheniformis in ion exchange resin.					
33937931	6	89	dep	solution	1087:1094	arg1	pH					1096:1097	pH 9.0	1096:1101	a buffer solution pH 9.0 at 25 °C for 24 h	1078:1119	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	2	90	theme	biotechnology	316:328	arg1	industries					330:339	the food, pharma, and biotechnology industries	294:339	the food, pharma, and biotechnology industries	294:339	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	1	91	theme	dairy	223:227	arg1	products					229:236	dairy products	223:236	dairy products	223:236	The food industry has developed a wide range of products with reduced lactose to allow people with intolerance to consume dairy products.					
33937931	6	92	theme	buffer	1080:1085	arg1	solution					1087:1094	a buffer solution	1078:1094	a buffer solution pH 9.0 at 25 °C for 24 h	1078:1119	Subsequently, experiments were performed to provide additional stabilization for biocatalyst, using a buffer solution pH 9.0 at 25 °C for 24 h, and crosslinking with different concentrations of glutaraldehyde.					
33937931	2	93	theme	high-cost	358:366	arg1	enzymes					346:352	the enzymes	342:352	the enzymes	342:352	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
33937931	2	93	theme	high-cost	358:366	arg1	catalysts					368:376	high-cost catalysts	358:376	high-cost catalysts	358:376	Although β-galactosidase has extensive applications in the food, pharma, and biotechnology industries, the enzymes are high-cost catalysts, and their use makes the process costly.					
32931715	2	0	theme	FA	340:341	arg1	profiles					343:350	FA profiles	340:350	FA profiles	340:350	However, the regulatory mechanisms ultimately resulting in FA profiles vary among animals and remain largely unknown.					
32931715	3	1	from	tissues	514:520	arg1	data					470:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data	420:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	420:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	1	from	tissues	514:520	arg1	samples					482:488	188 samples	478:488	188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	478:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	6	2	from	functions	1151:1159	arg1	tissues					1169:1175	four tissues	1164:1175	four tissues	1164:1175	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	8	3	from	FAs	1509:1511	arg1	cattle					1521:1526	beef cattle	1516:1526	beef cattle	1516:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	3	4	theme	regulatory	621:630	arg1	mechanisms					632:641	the molecular regulatory mechanisms	607:641	the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle	607:697	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	6	5	from	genes	1114:1118	arg1	tissues					1169:1175	four tissues	1164:1175	four tissues	1164:1175	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	8	6	theme	AS	1397:1398	arg1	events					1400:1405	AS events	1397:1405	AS events based on comprehensive RNA-Seq analysis	1397:1445	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	1	7	theme	urgent	229:234	arg1	tasks					236:240	the urgent tasks	225:240	the urgent tasks required to address consumer concerns	225:278	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	0	8	theme	regulatory	93:102	arg1	signatures					104:113	the regulatory signatures	89:113	the regulatory signatures of fatty acid composition in cattle	89:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	3	9	from	data	470:473	arg1	backfat					549:555	backfat	549:555	backfat	549:555	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	9	from	data	470:473	arg1	liver					530:534	liver	530:534	liver	530:534	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	9	from	data	470:473	arg1	rumen					523:527	rumen	523:527	rumen	523:527	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	9	from	data	470:473	arg1	tissues					514:520	four key metabolic tissues	495:520	four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	495:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	9	from	data	470:473	arg1	muscle					537:542	muscle	537:542	muscle	537:542	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	4	10	theme	regulatory	805:814	arg1	mechanisms					816:825	the transcriptional regulatory mechanisms	785:825	the transcriptional regulatory mechanisms in four tissues	785:841	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	5	11	theme	AS	892:893	arg1	events					895:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	5	12	theme	rumen	919:923	arg1	tissue					925:930	rumen tissue	919:930	rumen tissue	919:930	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	1	13	theme	tasks	236:240	arg1	one					218:220	one	218:220	one	218:220	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	1	13	theme	tasks	236:240	arg1	tasks					236:240	the urgent tasks	225:240	the urgent tasks required to address consumer concerns	225:278	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	4	14	theme	transcriptional	789:803	arg1	mechanisms					816:825	the transcriptional regulatory mechanisms	785:825	the transcriptional regulatory mechanisms in four tissues	785:841	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	3	15	theme	samples	482:488	arg1	data					470:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data	420:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	420:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	0	16	from	cattle	144:149	arg1	signatures					104:113	the regulatory signatures	89:113	the regulatory signatures of fatty acid composition in cattle	89:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	3	17	from	FAs	591:593	arg1	backfat					598:604	backfat	598:604	backfat	598:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	18	theme	FA	676:677	arg1	formation					679:687	FA formation	676:687	FA formation in cattle	676:697	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	19	from	formation	679:687	arg1	cattle					692:697	cattle	692:697	cattle	692:697	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	20	theme	metabolic	504:512	arg1	backfat					549:555	backfat	549:555	backfat	549:555	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	20	theme	metabolic	504:512	arg1	liver					530:534	liver	530:534	liver	530:534	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	20	theme	metabolic	504:512	arg1	rumen					523:527	rumen	523:527	rumen	523:527	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	20	theme	metabolic	504:512	arg1	tissues					514:520	four key metabolic tissues	495:520	four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	495:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	20	theme	metabolic	504:512	arg1	muscle					537:542	muscle	537:542	muscle	537:542	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	1	21	theme	healthy/unhealthy	167:183	arg1	acid					191:194	healthy/unhealthy fatty acid	167:194	the healthy/unhealthy fatty acid (FA) ratio in meat	163:213	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	1	21	theme	healthy/unhealthy	167:183	arg1	FA					197:198	FA	197:198	FA	197:198	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	3	22	theme	tissues	652:658	arg1	mechanisms					632:641	the molecular regulatory mechanisms	607:641	the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle	607:697	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	8	23	theme	mechanism	1496:1504	arg1	understanding					1464:1476	our understanding	1460:1476	our understanding of the regulatory mechanism of FAs in beef cattle	1460:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	1	24	theme	fatty	185:189	arg1	acid					191:194	healthy/unhealthy fatty acid	167:194	the healthy/unhealthy fatty acid (FA) ratio in meat	163:213	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	1	24	theme	fatty	185:189	arg1	FA					197:198	FA	197:198	FA	197:198	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	8	25	theme	comprehensive	1416:1428	arg1	analysis					1438:1445	comprehensive RNA-Seq analysis	1416:1445	comprehensive RNA-Seq analysis	1416:1445	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	8	26	theme	regulatory	1485:1494	arg1	mechanism					1496:1504	the regulatory mechanism	1481:1504	the regulatory mechanism of FAs in beef cattle	1481:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	3	27	from	backfat	598:604	arg1	contents					576:583	the contents	572:583	the contents of 49 FAs in backfat	572:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	28	from	contents	576:583	arg1	backfat					598:604	backfat	598:604	backfat	598:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	1	29	theme	acid	191:194	arg1	ratio					201:205	the healthy/unhealthy fatty acid (FA) ratio	163:205	the healthy/unhealthy fatty acid (FA) ratio in meat	163:213	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	0	30	theme	Gene	0:3	arg1	co-expression					5:17	Gene co-expression	0:17	Gene co-expression	0:17	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	7	31	theme	low	1317:1319	arg1	ratios					1342:1347	high and low healthy/unhealthy FA ratios	1308:1347	high and low healthy/unhealthy FA ratios	1308:1347	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	5	32	theme	conserved	871:879	arg1	events					895:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	7	33	theme	healthy/unhealthy	1321:1337	arg1	ratios					1342:1347	high and low healthy/unhealthy FA ratios	1308:1347	high and low healthy/unhealthy FA ratios	1308:1347	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	0	34	theme	alternative	23:33	arg1	splicing					35:42	alternative splicing	23:42	alternative splicing analysis	23:51	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	8	35	from	cattle	1521:1526	arg1	mechanism					1496:1504	the regulatory mechanism	1481:1504	the regulatory mechanism of FAs in beef cattle	1481:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	0	36	theme	acid	124:127	arg1	composition					129:139	fatty acid composition	118:139	fatty acid composition in cattle	118:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	6	37	theme	co-expressed	1121:1132	arg1	genes					1134:1138	co-expressed genes	1121:1138	co-expressed genes	1121:1138	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	6	38	theme	related	1067:1073	arg1	ratio					1061:1065	the healthy/unhealthy FA ratio	1036:1065	the healthy/unhealthy FA ratio related AS events	1036:1083	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	6	38	theme	related	1067:1073	arg1	addition					1026:1033	addition	1026:1033	addition	1026:1033	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	3	39	theme	high-quality	428:439	arg1	data					470:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data	420:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	420:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	5	40	located	detected	907:914	arg1	tissue					925:930	rumen tissue	919:930	rumen tissue	919:930	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	5	40	located	detected	907:914	arg2	events					895:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	0	41	theme	fatty	118:122	arg1	composition					129:139	fatty acid composition	118:139	fatty acid composition in cattle	118:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	3	42	theme	FAs	591:593	arg1	contents					576:583	the contents	572:583	the contents of 49 FAs in backfat	572:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	5	43	theme	absent	885:890	arg1	events					895:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events	860:900	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	6	44	dep	expressed	1099:1107	arg1	DE					1110:1111	DE	1110:1111	DE	1110:1111	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	3	45	theme	RNA-Seq-based	441:453	arg1	data					470:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data	420:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	420:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	7	46	theme	key	1198:1200	arg1	genes					1202:1206	Eight key genes	1192:1206	Eight key genes	1192:1206	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	6	47	theme	healthy/unhealthy	1040:1056	arg1	ratio					1061:1065	the healthy/unhealthy FA ratio	1036:1065	the healthy/unhealthy FA ratio related AS events	1036:1083	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	6	47	theme	healthy/unhealthy	1040:1056	arg1	addition					1026:1033	addition	1026:1033	addition	1026:1033	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	4	48	theme	splicing	756:763	arg1	mechanisms					816:825	the transcriptional regulatory mechanisms	785:825	the transcriptional regulatory mechanisms in four tissues	785:841	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	4	48	theme	splicing	756:763	arg1	one					778:780	one	778:780	one	778:780	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	4	48	theme	splicing	756:763	arg1	events					770:775	the alternative splicing (AS) events	740:775	the alternative splicing (AS) events	740:775	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	4	49	dep	splicing	756:763	arg1	AS					766:767	AS	766:767	AS	766:767	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	0	50	theme	splicing	35:42	arg1	analysis					44:51	alternative splicing analysis	23:51	alternative splicing analysis	23:51	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	0	51	theme	composition	129:139	arg1	signatures					104:113	the regulatory signatures	89:113	the regulatory signatures of fatty acid composition in cattle	89:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	5	52	theme	other	1002:1006	arg1	tissues					1014:1020	the other three tissues	998:1020	the other three tissues	998:1020	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	4	53	theme	alternative	744:754	arg1	splicing					756:763	alternative splicing	744:763	the alternative splicing (AS) events	740:775	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	1	54	from	ratio	201:205	arg1	meat					210:213	meat	210:213	meat	210:213	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	3	55	theme	Tb	425:426	arg1	data					470:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data	420:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	420:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	0	56	theme	key	56:58	arg1	tissues					70:76	key metabolic tissues	56:76	key metabolic tissues	56:76	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	5	57	theme	functional	961:970	arg1	differences					972:982	its functional differences	957:982	its functional differences	957:982	The highly conserved and absent AS events were detected in rumen tissue, which may contribute to its functional differences compared with the other three tissues.					
32931715	7	58	theme	FA	1339:1340	arg1	ratios					1342:1347	high and low healthy/unhealthy FA ratios	1308:1347	high and low healthy/unhealthy FA ratios	1308:1347	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	6	59	theme	FA	1058:1059	arg1	ratio					1061:1065	the healthy/unhealthy FA ratio	1036:1065	the healthy/unhealthy FA ratio related AS events	1036:1083	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	6	59	theme	FA	1058:1059	arg1	addition					1026:1033	addition	1026:1033	addition	1026:1033	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	8	60	theme	RNA-Seq	1430:1436	arg1	analysis					1438:1445	comprehensive RNA-Seq analysis	1416:1445	comprehensive RNA-Seq analysis	1416:1445	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	6	61	theme	expressed	1099:1107	arg1	genes					1114:1118	differential expressed (DE) genes	1086:1118	differential expressed (DE) genes	1086:1118	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	4	62	theme	mechanisms	816:825	arg1	mechanisms					816:825	the transcriptional regulatory mechanisms	785:825	the transcriptional regulatory mechanisms in four tissues	785:841	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	4	62	theme	mechanisms	816:825	arg1	one					778:780	one	778:780	one	778:780	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	4	62	theme	mechanisms	816:825	arg1	events					770:775	the alternative splicing (AS) events	740:775	the alternative splicing (AS) events	740:775	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	7	63	with	animals	1295:1301	arg1	ratios					1342:1347	high and low healthy/unhealthy FA ratios	1308:1347	high and low healthy/unhealthy FA ratios	1308:1347	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	3	64	theme	molecular	611:619	arg1	mechanisms					632:641	the molecular regulatory mechanisms	607:641	the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle	607:697	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	7	65	theme	integrated	1233:1242	arg1	analysis					1244:1251	the integrated analysis	1229:1251	the integrated analysis of DE	1229:1257	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	3	66	dep	tissues	514:520	arg1	backfat					549:555	backfat	549:555	backfat	549:555	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	66	dep	tissues	514:520	arg1	liver					530:534	liver	530:534	liver	530:534	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	66	dep	tissues	514:520	arg1	rumen					523:527	rumen	523:527	rumen	523:527	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	66	dep	tissues	514:520	arg1	tissues					514:520	four key metabolic tissues	495:520	four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	495:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	66	dep	tissues	514:520	arg1	muscle					537:542	muscle	537:542	muscle	537:542	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	6	67	from	genes	1134:1138	arg1	tissues					1169:1175	four tissues	1164:1175	four tissues	1164:1175	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	8	68	theme	beef	1516:1519	arg1	cattle					1521:1526	beef cattle	1516:1526	beef cattle	1516:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	0	69	from	signatures	104:113	arg1	cattle					144:149	cattle	144:149	cattle	144:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	7	70	theme	DE	1256:1257	arg1	analysis					1244:1251	the integrated analysis	1229:1251	the integrated analysis of DE	1229:1257	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	7	71	theme	high	1308:1311	arg1	ratios					1342:1347	high and low healthy/unhealthy FA ratios	1308:1347	high and low healthy/unhealthy FA ratios	1308:1347	Eight key genes were identified from the integrated analysis of DE, co-expressed, and AS genes between animals with high and low healthy/unhealthy FA ratios.					
32931715	3	72	theme	transcriptomic	455:468	arg1	data					470:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data	420:473	~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	420:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	8	73	from	mechanism	1496:1504	arg1	cattle					1521:1526	beef cattle	1516:1526	beef cattle	1516:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	8	74	theme	applicable	1373:1382	arg1	pipeline					1384:1391	an applicable pipeline	1370:1391	an applicable pipeline for AS events based on comprehensive RNA-Seq analysis	1370:1445	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
32931715	4	75	theme	large	725:729	arg1	dataset					731:737	this large dataset	720:737	this large dataset	720:737	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	2	76	theme	regulatory	294:303	arg1	mechanisms					305:314	the regulatory mechanisms	290:314	the regulatory mechanisms ultimately resulting in FA profiles	290:350	However, the regulatory mechanisms ultimately resulting in FA profiles vary among animals and remain largely unknown.					
32931715	0	77	from	composition	129:139	arg1	cattle					144:149	cattle	144:149	cattle	144:149	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	0	78	theme	tissues	70:76	arg1	analysis					44:51	alternative splicing analysis	23:51	alternative splicing analysis	23:51	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	0	78	theme	tissues	70:76	arg1	co-expression					5:17	Gene co-expression	0:17	Gene co-expression	0:17	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	4	79	from	mechanisms	816:825	arg1	tissues					835:841	four tissues	830:841	four tissues	830:841	Using this large dataset, the alternative splicing (AS) events, one of the transcriptional regulatory mechanisms in four tissues were identified.					
32931715	6	80	theme	differential	1086:1097	arg1	genes					1114:1118	differential expressed (DE) genes	1086:1118	differential expressed (DE) genes	1086:1118	In addition, the healthy/unhealthy FA ratio related AS events, differential expressed (DE) genes, co-expressed genes, and their functions in four tissues were analysed.					
32931715	0	81	theme	metabolic	60:68	arg1	tissues					70:76	key metabolic tissues	56:76	key metabolic tissues	56:76	Gene co-expression and alternative splicing analysis of key metabolic tissues to unravel the regulatory signatures of fatty acid composition in cattle.					
32931715	1	82	dep	consumer	262:269	arg1	concerns					271:278	concerns	271:278	concerns	271:278	Increasing the healthy/unhealthy fatty acid (FA) ratio in meat is one of the urgent tasks required to address consumer concerns.					
32931715	3	83	theme	key	500:502	arg1	backfat					549:555	backfat	549:555	backfat	549:555	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	83	theme	key	500:502	arg1	liver					530:534	liver	530:534	liver	530:534	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	83	theme	key	500:502	arg1	rumen					523:527	rumen	523:527	rumen	523:527	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	83	theme	key	500:502	arg1	tissues					514:520	four key metabolic tissues	495:520	four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat	495:604	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	3	83	theme	key	500:502	arg1	muscle					537:542	muscle	537:542	muscle	537:542	In this study, using ~1.2 Tb high-quality RNA-Seq-based transcriptomic data of 188 samples from four key metabolic tissues (rumen, liver, muscle, and backfat) together with the contents of 49 FAs in backfat, the molecular regulatory mechanisms of these tissues contributing to FA formation in cattle were explored.					
32931715	8	84	theme	FAs	1509:1511	arg1	mechanism					1496:1504	the regulatory mechanism	1481:1504	the regulatory mechanism of FAs in beef cattle	1481:1526	This study provides an applicable pipeline for AS events based on comprehensive RNA-Seq analysis and improves our understanding of the regulatory mechanism of FAs in beef cattle.					
34321544	10	0	from	plants	1060:1065	arg1	Seeds					1037:1041	Seeds	1037:1041	Seeds from the treated plants	1037:1065	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	11	1	theme	fatty	1270:1274	arg1	composition					1281:1291	fatty acid composition	1270:1291	fatty acid composition	1270:1291	In addition, they featured changes in their amino acid profile and fatty acid composition.					
34321544	8	2	theme	biometric	971:979	arg1	traits					981:986	biometric traits	971:986	biometric traits	971:986	The extracts modified soybean plant physiology, as manifested by changes in biometric traits.					
34321544	10	3	contain	had	1067:1069	arg2	contents					1078:1085	higher contents	1071:1085	higher contents of micro- and macroelements	1071:1113	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	10	3	contain	had	1067:1069	arg1	Seeds					1037:1041	Seeds	1037:1041	Seeds from the treated plants	1037:1065	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	10	3	contain	had	1067:1069	arg2	concentrations					1133:1146	total concentrations	1127:1146	total concentrations of lipids	1127:1156	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	6	4	contain	had	659:661	arg2	contents					668:675	high contents	663:675	high contents of polyphenolic compounds and rich micro- and macroelemental composition	663:748	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	6	4	contain	had	659:661	arg1	extracts					650:657	Lovage extracts	643:657	Lovage extracts	643:657	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	10	5	theme	micro-	1090:1095	arg1	concentrations					1133:1146	total concentrations	1127:1146	total concentrations of lipids	1127:1156	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	10	5	theme	micro-	1090:1095	arg1	contents					1078:1085	higher contents	1071:1085	higher contents of micro- and macroelements	1071:1113	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	11	6	theme	acid	1276:1279	arg1	composition					1281:1291	fatty acid composition	1270:1291	fatty acid composition	1270:1291	In addition, they featured changes in their amino acid profile and fatty acid composition.					
34321544	3	7	theme	biochemical	499:509	arg1	indicators					511:520	biochemical indicators	499:520	biochemical indicators	499:520	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	10	8	from	decrease	1173:1180	arg1	content					1193:1199	protein content	1185:1199	protein content	1185:1199	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	0	9	theme	biostimulant	89:100	arg1	application					61:71	the application	57:71	the application of allelopathic biostimulant from Levisticum officinale Koch	57:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	3	10	from	roots	404:408	arg1	cultivation					421:431	soybean cultivation	413:431	soybean cultivation	413:431	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	5	11	theme	foliar	604:609	arg1	application					611:621	foliar application	604:621	foliar application	604:621	The extracts were administered via foliar application and soil treatment.					
34321544	1	12	theme	natural	256:262	arg1	preparations					264:275	natural preparations	256:275	natural preparations	256:275	The interest expressed by the agriculture in the category of innovative biostimulants is due to the intensive search for natural preparations.					
34321544	0	13	theme	allelopathic	76:87	arg1	biostimulant					89:100	allelopathic biostimulant	76:100	allelopathic biostimulant from Levisticum officinale Koch	76:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	13	14	from	officinale	1442:1451	arg1	biostimulants					1412:1424	The natural biostimulants	1400:1424	The natural biostimulants from Levisticum officinale	1400:1451	The natural biostimulants from Levisticum officinale may become a valuable tool in the sustainable agriculture.					
34321544	6	15	theme	macroelemental	723:736	arg1	composition					738:748	macroelemental composition	723:748	macroelemental composition	723:748	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	11	16	from	changes	1230:1236	arg1	profile					1258:1264	their amino acid profile	1241:1264	their amino acid profile	1241:1264	In addition, they featured changes in their amino acid profile and fatty acid composition.					
34321544	11	16	from	changes	1230:1236	arg1	composition					1281:1291	fatty acid composition	1270:1291	fatty acid composition	1270:1291	In addition, they featured changes in their amino acid profile and fatty acid composition.					
34321544	0	17	theme	Levisticum	107:116	arg1	Koch					129:132	Levisticum officinale Koch	107:132	Levisticum officinale Koch	107:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	5	18	theme	soil	627:630	arg1	treatment					632:640	soil treatment	627:640	soil treatment	627:640	The extracts were administered via foliar application and soil treatment.					
34321544	3	19	theme	morphological	455:467	arg1	observations					469:480	morphological observations	455:480	morphological observations	455:480	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	10	20	theme	slight	1166:1171	arg1	decrease					1173:1180	a slight decrease	1164:1180	a slight decrease in protein content	1164:1199	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	2	21	theme	Levisticum	376:385	arg1	Koch					398:401	Levisticum officinale Koch	376:401	Levisticum officinale Koch	376:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	10	22	theme	protein	1185:1191	arg1	content					1193:1199	protein content	1185:1199	protein content	1185:1199	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	6	23	theme	micro-	712:717	arg1	contents					668:675	high contents	663:675	high contents of polyphenolic compounds and rich micro- and macroelemental composition	663:748	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	13	24	theme	Levisticum	1431:1440	arg1	officinale					1442:1451	Levisticum officinale	1431:1451	Levisticum officinale	1431:1451	The natural biostimulants from Levisticum officinale may become a valuable tool in the sustainable agriculture.					
34321544	10	25	theme	lipids	1151:1156	arg1	concentrations					1133:1146	total concentrations	1127:1146	total concentrations of lipids	1127:1156	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	10	25	theme	lipids	1151:1156	arg1	contents					1078:1085	higher contents	1071:1085	higher contents of micro- and macroelements	1071:1113	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	12	26	theme	saponins	1390:1397	arg1	concentrations					1356:1369	increased concentrations	1346:1369	increased concentrations of isoflavones and saponins	1346:1397	The application of allelopathic biostimulant caused increased concentrations of isoflavones and saponins.					
34321544	6	27	theme	rich	707:710	arg1	micro-					712:717	rich micro-	707:717	rich micro-	707:717	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	2	28	theme	extracts	362:369	arg1	use					342:344	the use	338:344	the use of allelopathic extracts from Levisticum officinale Koch	338:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	7	29	theme	abscisic	831:838	arg1	acid					840:843	the abscisic acid	827:843	the abscisic acid	827:843	The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found.					
34321544	13	30	theme	natural	1404:1410	arg1	biostimulants					1412:1424	The natural biostimulants	1400:1424	The natural biostimulants from Levisticum officinale	1400:1451	The natural biostimulants from Levisticum officinale may become a valuable tool in the sustainable agriculture.					
34321544	8	31	theme	soybean	917:923	arg1	physiology					931:940	soybean plant physiology	917:940	soybean plant physiology	917:940	The extracts modified soybean plant physiology, as manifested by changes in biometric traits.					
34321544	2	32	theme	allelopathic	349:360	arg1	extracts					362:369	allelopathic extracts	349:369	allelopathic extracts from Levisticum officinale Koch	349:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	4	33	theme	extraction	545:554	arg1	method					527:532	Hot method	523:532	Hot method of aqueous extraction	523:554	Hot method of aqueous extraction was applied.					
34321544	2	34	from	use	342:344	arg1	Koch					398:401	Levisticum officinale Koch	376:401	Levisticum officinale Koch	376:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	6	35	theme	compounds	693:701	arg1	contents					668:675	high contents	663:675	high contents of polyphenolic compounds and rich micro- and macroelemental composition	663:748	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	13	36	theme	sustainable	1487:1497	arg1	agriculture					1499:1509	the sustainable agriculture	1483:1509	the sustainable agriculture	1483:1509	The natural biostimulants from Levisticum officinale may become a valuable tool in the sustainable agriculture.					
34321544	0	37	theme	multi-level	15:25	arg1	response					27:34	the multi-level response	11:34	the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch	11:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	0	38	theme	officinale	118:127	arg1	Koch					129:132	Levisticum officinale Koch	107:132	Levisticum officinale Koch	107:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	10	39	theme	treated	1052:1058	arg1	plants					1060:1065	the treated plants	1048:1065	the treated plants	1048:1065	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	8	40	theme	plant	925:929	arg1	physiology					931:940	soybean plant physiology	917:940	soybean plant physiology	917:940	The extracts modified soybean plant physiology, as manifested by changes in biometric traits.					
34321544	6	41	theme	polyphenolic	680:691	arg1	compounds					693:701	polyphenolic compounds	680:701	polyphenolic compounds	680:701	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	1	42	theme	innovative	196:205	arg1	biostimulants					207:219	innovative biostimulants	196:219	innovative biostimulants	196:219	The interest expressed by the agriculture in the category of innovative biostimulants is due to the intensive search for natural preparations.					
34321544	0	43	theme	Glycine	39:45	arg1	L.					51:52	Glycine max L.	39:52	Glycine max L.	39:52	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	10	44	theme	total	1127:1131	arg1	concentrations					1133:1146	total concentrations	1127:1146	total concentrations of lipids	1127:1156	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	0	45	from	application	61:71	arg1	Koch					129:132	Levisticum officinale Koch	107:132	Levisticum officinale Koch	107:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	3	46	theme	soybean	413:419	arg1	cultivation					421:431	soybean cultivation	413:431	soybean cultivation	413:431	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	1	47	theme	biostimulants	207:219	arg1	category					184:191	the category	180:191	the category of innovative biostimulants	180:219	The interest expressed by the agriculture in the category of innovative biostimulants is due to the intensive search for natural preparations.					
34321544	9	48	theme	increased	1020:1028	arg1	yield					1030:1034	increased yield	1020:1034	increased yield	1020:1034	Plants responded positively by increased yield.					
34321544	7	49	theme	indole-3-acetic	802:816	arg1	acid					818:821	indole-3-acetic acid	802:821	indole-3-acetic acid	802:821	The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found.					
34321544	12	50	theme	increased	1346:1354	arg1	concentrations					1356:1369	increased concentrations	1346:1369	increased concentrations of isoflavones and saponins	1346:1397	The application of allelopathic biostimulant caused increased concentrations of isoflavones and saponins.					
34321544	2	51	theme	officinale	387:396	arg1	Koch					398:401	Levisticum officinale Koch	376:401	Levisticum officinale Koch	376:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	0	52	theme	L.	51:52	arg1	response					27:34	the multi-level response	11:34	the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch	11:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	4	53	theme	Hot	523:525	arg1	method					527:532	Hot method	523:532	Hot method of aqueous extraction	523:554	Hot method of aqueous extraction was applied.					
34321544	11	54	theme	amino	1247:1251	arg1	profile					1258:1264	their amino acid profile	1241:1264	their amino acid profile	1241:1264	In addition, they featured changes in their amino acid profile and fatty acid composition.					
34321544	12	55	theme	isoflavones	1374:1384	arg1	concentrations					1356:1369	increased concentrations	1346:1369	increased concentrations of isoflavones and saponins	1346:1397	The application of allelopathic biostimulant caused increased concentrations of isoflavones and saponins.					
34321544	13	56	theme	valuable	1466:1473	arg1	tool					1475:1478	a valuable tool	1464:1478	a valuable tool	1464:1478	The natural biostimulants from Levisticum officinale may become a valuable tool in the sustainable agriculture.					
34321544	0	57	theme	max	47:49	arg1	L.					51:52	Glycine max L.	39:52	Glycine max L.	39:52	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	4	58	theme	aqueous	537:543	arg1	extraction					545:554	aqueous extraction	537:554	aqueous extraction	537:554	Hot method of aqueous extraction was applied.					
34321544	11	59	theme	acid	1253:1256	arg1	profile					1258:1264	their amino acid profile	1241:1264	their amino acid profile	1241:1264	In addition, they featured changes in their amino acid profile and fatty acid composition.					
34321544	2	60	theme	complex	318:324	arg1	approach					326:333	a complex approach	316:333	a complex approach to the use of allelopathic extracts from Levisticum officinale Koch	316:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	12	61	theme	biostimulant	1326:1337	arg1	application					1298:1308	The application	1294:1308	The application of allelopathic biostimulant	1294:1337	The application of allelopathic biostimulant caused increased concentrations of isoflavones and saponins.					
34321544	10	62	theme	higher	1071:1076	arg1	contents					1078:1085	higher contents	1071:1085	higher contents of micro- and macroelements	1071:1113	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	12	63	theme	allelopathic	1313:1324	arg1	biostimulant					1326:1337	allelopathic biostimulant	1313:1337	allelopathic biostimulant	1313:1337	The application of allelopathic biostimulant caused increased concentrations of isoflavones and saponins.					
34321544	3	64	theme	observations	469:480	arg1	analyses					443:450	analyses	443:450	analyses of morphological observations	443:480	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	3	64	theme	observations	469:480	arg1	analyses					487:494	analyses	487:494	analyses of biochemical indicators	487:520	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	6	65	theme	high	663:666	arg1	contents					668:675	high contents	663:675	high contents of polyphenolic compounds and rich micro- and macroelemental composition	663:748	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	2	66	from	Koch	398:401	arg1	use					342:344	the use	338:344	the use of allelopathic extracts from Levisticum officinale Koch	338:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	2	66	from	Koch	398:401	arg1	extracts					362:369	allelopathic extracts	349:369	allelopathic extracts from Levisticum officinale Koch	349:401	Our study is the first ever to report a complex approach to the use of allelopathic extracts from Levisticum officinale Koch.					
34321544	7	67	theme	gibberellic	781:791	arg1	acid					793:796	gibberellic acid	781:796	gibberellic acid	781:796	The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found.					
34321544	8	68	from	changes	960:966	arg1	traits					981:986	biometric traits	971:986	biometric traits	971:986	The extracts modified soybean plant physiology, as manifested by changes in biometric traits.					
34321544	3	69	theme	indicators	511:520	arg1	analyses					443:450	analyses	443:450	analyses of morphological observations	443:480	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	3	69	theme	indicators	511:520	arg1	analyses					487:494	analyses	487:494	analyses of biochemical indicators	487:520	roots in soybean cultivation, includes analyses of morphological observations, and analyses of biochemical indicators.					
34321544	0	70	from	Koch	129:132	arg1	application					61:71	the application	57:71	the application of allelopathic biostimulant from Levisticum officinale Koch	57:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	0	70	from	Koch	129:132	arg1	biostimulant					89:100	allelopathic biostimulant	76:100	allelopathic biostimulant from Levisticum officinale Koch	76:132	Uncovering the multi-level response of Glycine max L. to the application of allelopathic biostimulant from Levisticum officinale Koch.					
34321544	10	71	theme	macroelements	1101:1113	arg1	concentrations					1133:1146	total concentrations	1127:1146	total concentrations of lipids	1127:1156	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	10	71	theme	macroelements	1101:1113	arg1	contents					1078:1085	higher contents	1071:1085	higher contents of micro- and macroelements	1071:1113	Seeds from the treated plants had higher contents of micro- and macroelements, as well as total concentrations of lipids (with a slight decrease in protein content).					
34321544	7	72	contain	contain	773:779	arg2	acid					793:796	gibberellic acid	781:796	gibberellic acid	781:796	The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found.					
34321544	7	72	contain	contain	773:779	arg1	infusions					755:763	The infusions	751:763	The infusions	751:763	The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found.					
34321544	7	72	contain	contain	773:779	arg2	acid					818:821	indole-3-acetic acid	802:821	indole-3-acetic acid	802:821	The infusions did not contain gibberellic acid and indole-3-acetic acid but the abscisic acid and saccharose, glucose, and fructose were found.					
34321544	1	73	from	agriculture	165:175	arg1	category					184:191	the category	180:191	the category of innovative biostimulants	180:219	The interest expressed by the agriculture in the category of innovative biostimulants is due to the intensive search for natural preparations.					
34321544	6	74	theme	Lovage	643:648	arg1	extracts					650:657	Lovage extracts	643:657	Lovage extracts	643:657	Lovage extracts had high contents of polyphenolic compounds and rich micro- and macroelemental composition.					
34321544	1	75	theme	intensive	235:243	arg1	search					245:250	the intensive search	231:250	the intensive search for natural preparations	231:275	The interest expressed by the agriculture in the category of innovative biostimulants is due to the intensive search for natural preparations.					
35098226	0	0	theme	BO62316	79:85	arg1	Haptens					39:45	the Tetrasaccharide Haptens	19:45	the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316	19:85	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	8	1	theme	high	1227:1230	arg1	efficacy					1232:1239	their high efficacy to induce robust T-cell-dependent immune responses	1221:1290	their high efficacy to induce robust T-cell-dependent immune responses	1221:1290	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	8	2	theme	glycoconjugates	1188:1202	arg1	studies					1163:1169	Preliminary immunological studies	1137:1169	Preliminary immunological studies of the resultant glycoconjugates in mice	1137:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	1	3	theme	high	249:252	arg1	rate					261:264	a high lethal rate	247:264	a high lethal rate exceeding 50%	247:278	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	2	4	theme	clinical	398:405	arg1	infections					407:416	the clinical infections	394:416	the clinical infections	394:416	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	4	5	theme	CPSs	730:733	arg1	units					688:692	units	688:692	units of V. vulnificus MO6-24 and BO62316 CPSs	688:733	This article describes the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs.					
35098226	1	6	theme	lethal	254:259	arg1	rate					261:264	a high lethal rate	247:264	a high lethal rate exceeding 50%	247:278	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	0	7	theme	Immunological	91:103	arg1	Evaluation					105:114	Immunological Evaluation	91:114	Immunological Evaluation of Their Protein Conjugates	91:142	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	2	8	theme	V.	281:282	arg1	MO6-24					295:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	8	theme	V.	281:282	arg1	strains					343:349	two predominant virulent strains	318:349	two predominant virulent strains associated with approximately one-third of the clinical infections	318:416	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	8	theme	V.	281:282	arg1	BO62316					306:312	BO62316	306:312	BO62316	306:312	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	4	9	theme	first	621:625	arg1	synthesis					633:641	the first total synthesis	617:641	the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs	617:733	This article describes the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs.					
35098226	5	10	theme	synthesis	758:766	arg1	feature					742:748	A key feature	736:748	A key feature of this synthesis	736:766	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	5	10	theme	synthesis	758:766	arg1	assembly					776:783	the assembly	772:783	the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor	772:872	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	6	11	from	α-d-GalAN	964:972	arg1	disaccharides					986:998	disaccharides	986:998	not only disaccharides but also tri- and tetrasaccharides	977:1033	A modified TEMPO/BAIB oxidation protocol was developed to directly convert α-d-GalN into α-d-GalAN in not only disaccharides but also tri- and tetrasaccharides.					
35098226	6	11	from	α-d-GalAN	964:972	arg1	tri-					1009:1012	tri-	1009:1012	tri-	1009:1012	A modified TEMPO/BAIB oxidation protocol was developed to directly convert α-d-GalN into α-d-GalAN in not only disaccharides but also tri- and tetrasaccharides.					
35098226	3	12	theme	effective	559:567	arg1	vaccines					584:591	effective glycoconjugate vaccines	559:591	effective glycoconjugate vaccines	559:591	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	8	13	theme	weak	1355:1358	arg1	cross-reactivity					1360:1375	weak cross-reactivity	1355:1375	weak cross-reactivity with the other synthetic tetrasaccharide	1355:1416	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	8	14	theme	robust	1251:1256	arg1	responses					1282:1290	robust T-cell-dependent immune responses	1251:1290	robust T-cell-dependent immune responses	1251:1290	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	8	15	theme	other	1386:1390	arg1	tetrasaccharide					1402:1416	the other synthetic tetrasaccharide	1382:1416	the other synthetic tetrasaccharide	1382:1416	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	4	16	theme	challenging	650:660	arg1	tetrasaccharide					662:676	the challenging tetrasaccharide	646:676	the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs	646:733	This article describes the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs.					
35098226	1	17	theme	human	168:172	arg1	vulnificus					152:161	Vibrio vulnificus	145:161	Vibrio vulnificus	145:161	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	1	17	theme	human	168:172	arg1	pathogen					174:181	a human pathogen	166:181	a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%	166:278	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	8	18	theme	IgG	1301:1303	arg1	antibodies					1305:1314	the IgG antibodies	1297:1314	the IgG antibodies elicited by each glycoconjugate	1297:1346	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	5	19	theme	skeleton	808:815	arg1	feature					742:748	A key feature	736:748	A key feature of this synthesis	736:766	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	5	19	theme	skeleton	808:815	arg1	assembly					776:783	the assembly	772:783	the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor	772:872	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	8	20	from	mice	1207:1210	arg1	studies					1163:1169	Preliminary immunological studies	1137:1169	Preliminary immunological studies of the resultant glycoconjugates in mice	1137:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	8	21	theme	T-cell-dependent	1258:1273	arg1	responses					1282:1290	robust T-cell-dependent immune responses	1251:1290	robust T-cell-dependent immune responses	1251:1290	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	8	22	theme	immune	1275:1280	arg1	responses					1282:1290	robust T-cell-dependent immune responses	1251:1290	robust T-cell-dependent immune responses	1251:1290	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	5	23	theme	tetrasaccharide	792:806	arg1	skeleton					808:815	the tetrasaccharide skeleton	788:815	the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor	788:872	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	8	24	with	cross-reactivity	1360:1375	arg1	tetrasaccharide					1402:1416	the other synthetic tetrasaccharide	1382:1416	the other synthetic tetrasaccharide	1382:1416	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	0	25	theme	Total	0:4	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316	0:85	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	7	26	theme	bifunctional	1116:1127	arg1	linker					1129:1134	a bifunctional linker	1114:1134	a bifunctional linker	1114:1134	The synthetic haptens were covalently coupled with CRM197 carrier protein via a bifunctional linker.					
35098226	4	27	theme	vulnificus	700:709	arg1	units					688:692	units	688:692	units of V. vulnificus MO6-24 and BO62316 CPSs	688:733	This article describes the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs.					
35098226	0	28	theme	Protein	125:131	arg1	Conjugates					133:142	Their Protein Conjugates	119:142	Their Protein Conjugates	119:142	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	3	29	theme	glycoconjugate	569:582	arg1	vaccines					584:591	effective glycoconjugate vaccines	559:591	effective glycoconjugate vaccines	559:591	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	3	30	theme	vulnificus	461:470	arg1	CPSs					449:452	CPSs	449:452	CPSs	449:452	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	3	30	theme	vulnificus	461:470	arg1	polysaccharides					432:446	The capsular polysaccharides	419:446	The capsular polysaccharides (CPSs) of V. vulnificus	419:470	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	5	31	theme	glycosyl	859:866	arg1	donor					868:872	the glycosyl donor	855:872	the glycosyl donor	855:872	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	5	31	theme	glycosyl	859:866	arg1	trisaccharide					838:850	a 3,4-branched trisaccharide	823:850	a 3,4-branched trisaccharide	823:850	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	5	32	theme	key	738:740	arg1	feature					742:748	A key feature	736:748	A key feature of this synthesis	736:766	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	5	32	theme	key	738:740	arg1	assembly					776:783	the assembly	772:783	the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor	772:872	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	2	33	theme	vulnificus	284:293	arg1	MO6-24					295:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	33	theme	vulnificus	284:293	arg1	strains					343:349	two predominant virulent strains	318:349	two predominant virulent strains associated with approximately one-third of the clinical infections	318:416	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	33	theme	vulnificus	284:293	arg1	BO62316					306:312	BO62316	306:312	BO62316	306:312	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	1	34	theme	fatal	198:202	arg1	septicemia					204:213	fatal septicemia and necrotizing infections	198:240	septicemia	204:213	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	0	35	theme	Haptens	39:45	arg1	Evaluation					105:114	Immunological Evaluation	91:114	Immunological Evaluation of Their Protein Conjugates	91:142	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	0	35	theme	Haptens	39:45	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316	0:85	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	3	36	theme	unique	504:509	arg1	sugars					511:516	several structurally unique sugars	483:516	several structurally unique sugars	483:516	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	8	37	theme	Preliminary	1137:1147	arg1	studies					1163:1169	Preliminary immunological studies	1137:1169	Preliminary immunological studies of the resultant glycoconjugates in mice	1137:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	0	38	theme	Tetrasaccharide	23:37	arg1	Haptens					39:45	the Tetrasaccharide Haptens	19:45	the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316	19:85	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	0	39	theme	Conjugates	133:142	arg1	Evaluation					105:114	Immunological Evaluation	91:114	Immunological Evaluation of Their Protein Conjugates	91:142	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	0	39	theme	Conjugates	133:142	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316	0:85	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	6	40	theme	oxidation	897:905	arg1	protocol					907:914	A modified TEMPO/BAIB oxidation protocol	875:914	A modified TEMPO/BAIB oxidation protocol	875:914	A modified TEMPO/BAIB oxidation protocol was developed to directly convert α-d-GalN into α-d-GalAN in not only disaccharides but also tri- and tetrasaccharides.					
35098226	2	41	theme	infections	407:416	arg1	one-third					381:389	one-third	381:389	one-third	381:389	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	41	theme	infections	407:416	arg1	infections					407:416	the clinical infections	394:416	the clinical infections	394:416	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	0	42	theme	Vibrio	50:55	arg1	vulnificus					57:66	Vibrio vulnificus MO6-24 and BO62316	50:85	vulnificus	57:66	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	8	43	theme	resultant	1178:1186	arg1	glycoconjugates					1188:1202	the resultant glycoconjugates	1174:1202	the resultant glycoconjugates in mice	1174:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	6	44	theme	TEMPO/BAIB	886:895	arg1	protocol					907:914	A modified TEMPO/BAIB oxidation protocol	875:914	A modified TEMPO/BAIB oxidation protocol	875:914	A modified TEMPO/BAIB oxidation protocol was developed to directly convert α-d-GalN into α-d-GalAN in not only disaccharides but also tri- and tetrasaccharides.					
35098226	2	45	theme	virulent	334:341	arg1	MO6-24					295:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	45	theme	virulent	334:341	arg1	strains					343:349	two predominant virulent strains	318:349	two predominant virulent strains associated with approximately one-third of the clinical infections	318:416	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	45	theme	virulent	334:341	arg1	BO62316					306:312	BO62316	306:312	BO62316	306:312	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	5	46	theme	3,4-branched	825:836	arg1	donor					868:872	the glycosyl donor	855:872	the glycosyl donor	855:872	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	5	46	theme	3,4-branched	825:836	arg1	trisaccharide					838:850	a 3,4-branched trisaccharide	823:850	a 3,4-branched trisaccharide	823:850	A key feature of this synthesis was the assembly of the tetrasaccharide skeleton using a 3,4-branched trisaccharide as the glycosyl donor.					
35098226	8	47	theme	immunological	1149:1161	arg1	studies					1163:1169	Preliminary immunological studies	1137:1169	Preliminary immunological studies of the resultant glycoconjugates in mice	1137:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	6	48	theme	modified	877:884	arg1	protocol					907:914	A modified TEMPO/BAIB oxidation protocol	875:914	A modified TEMPO/BAIB oxidation protocol	875:914	A modified TEMPO/BAIB oxidation protocol was developed to directly convert α-d-GalN into α-d-GalAN in not only disaccharides but also tri- and tetrasaccharides.					
35098226	2	49	theme	predominant	322:332	arg1	MO6-24					295:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24	281:300	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	49	theme	predominant	322:332	arg1	strains					343:349	two predominant virulent strains	318:349	two predominant virulent strains associated with approximately one-third of the clinical infections	318:416	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	2	49	theme	predominant	322:332	arg1	BO62316					306:312	BO62316	306:312	BO62316	306:312	V. vulnificus MO6-24 and BO62316 are two predominant virulent strains associated with approximately one-third of the clinical infections.					
35098226	7	50	theme	CRM197	1087:1092	arg1	protein					1102:1108	CRM197 carrier protein	1087:1108	CRM197 carrier protein	1087:1108	The synthetic haptens were covalently coupled with CRM197 carrier protein via a bifunctional linker.					
35098226	8	51	from	studies	1163:1169	arg1	mice					1207:1210	mice	1207:1210	mice	1207:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	1	52	theme	necrotizing	219:229	arg1	infections					231:240	fatal septicemia and necrotizing infections	198:240	infections	231:240	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	7	53	theme	carrier	1094:1100	arg1	protein					1102:1108	CRM197 carrier protein	1087:1108	CRM197 carrier protein	1087:1108	The synthetic haptens were covalently coupled with CRM197 carrier protein via a bifunctional linker.					
35098226	7	54	theme	synthetic	1040:1048	arg1	haptens					1050:1056	The synthetic haptens	1036:1056	The synthetic haptens	1036:1056	The synthetic haptens were covalently coupled with CRM197 carrier protein via a bifunctional linker.					
35098226	3	55	theme	several	483:489	arg1	sugars					511:516	several structurally unique sugars	483:516	several structurally unique sugars	483:516	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	4	56	theme	total	627:631	arg1	synthesis					633:641	the first total synthesis	617:641	the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs	617:733	This article describes the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs.					
35098226	3	57	theme	V.	458:459	arg1	vulnificus					461:470	V. vulnificus	458:470	V. vulnificus	458:470	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	4	58	theme	tetrasaccharide	662:676	arg1	synthesis					633:641	the first total synthesis	617:641	the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs	617:733	This article describes the first total synthesis of the challenging tetrasaccharide repeating units of V. vulnificus MO6-24 and BO62316 CPSs.					
35098226	1	59	theme	Vibrio	145:150	arg1	vulnificus					152:161	Vibrio vulnificus	145:161	Vibrio vulnificus	145:161	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	1	59	theme	Vibrio	145:150	arg1	pathogen					174:181	a human pathogen	166:181	a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%	166:278	Vibrio vulnificus is a human pathogen that can cause fatal septicemia and necrotizing infections with a high lethal rate exceeding 50%.					
35098226	8	60	theme	synthetic	1392:1400	arg1	tetrasaccharide					1402:1416	the other synthetic tetrasaccharide	1382:1416	the other synthetic tetrasaccharide	1382:1416	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	0	61	theme	vulnificus	57:66	arg1	Haptens					39:45	the Tetrasaccharide Haptens	19:45	the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316	19:85	Total Synthesis of the Tetrasaccharide Haptens of Vibrio vulnificus MO6-24 and BO62316 and Immunological Evaluation of Their Protein Conjugates.					
35098226	3	62	theme	capsular	423:430	arg1	CPSs					449:452	CPSs	449:452	CPSs	449:452	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	3	62	theme	capsular	423:430	arg1	polysaccharides					432:446	The capsular polysaccharides	419:446	The capsular polysaccharides (CPSs) of V. vulnificus	419:470	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
35098226	8	63	from	glycoconjugates	1188:1202	arg1	mice					1207:1210	mice	1207:1210	mice	1207:1210	Preliminary immunological studies of the resultant glycoconjugates in mice revealed their high efficacy to induce robust T-cell-dependent immune responses, and the IgG antibodies elicited by each glycoconjugate showed weak cross-reactivity with the other synthetic tetrasaccharide.					
35098226	3	64	theme	excellent	526:534	arg1	targets					536:542	excellent targets	526:542	excellent targets for developing effective glycoconjugate vaccines	526:591	The capsular polysaccharides (CPSs) of V. vulnificus consist of several structurally unique sugars and are excellent targets for developing effective glycoconjugate vaccines.					
32580171	0	0	theme	bone	112:115	arg1	repair					124:129	bone defect repair	112:129	bone defect repair in osteoporosis rats	112:150	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	6	1	theme	in	881:882	arg1	time					902:905	the in vivo and in vitro degradation time	865:905	the in vivo and in vitro degradation time of CS-SrCSH microspheres	865:930	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	6	1	theme	in	881:882	arg1	longer					949:954	longer	949:954	longer	949:954	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	7	2	theme	CS-SrCSH-based	1067:1080	arg1	cement					1082:1087	CS-SrCSH-based cement	1067:1087	CS-SrCSH-based cement	1067:1087	Furthermore, CS-SrCSH-based cement is used to repair critical-sized OVX rat tibial defects.					
32580171	1	3	theme	-doped	167:172	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	6	4	theme	in	869:870	arg1	time					902:905	the in vivo and in vitro degradation time	865:905	the in vivo and in vitro degradation time of CS-SrCSH microspheres	865:930	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	6	4	theme	in	869:870	arg1	longer					949:954	longer	949:954	longer	949:954	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	1	5	theme	osteoporotic	264:275	arg1	repair					282:287	osteoporotic bone repair	264:287	osteoporotic bone repair	264:287	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	8	6	theme	in	1150:1151	arg1	results					1158:1164	The in vivo results	1146:1164	The in vivo results	1146:1164	The in vivo results reveal that CS-SrCSH exhibits a long-term capability for osteogenesis, angiogenesis and bone metabolism inhibition.					
32580171	1	7	theme	reduction	352:360	arg1	stimulation					316:326	the stimulation	312:326	the stimulation of bone formation and a reduction in bone resorption	312:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	1	8	from	formation	336:344	arg1	resorption					370:379	bone resorption	365:379	bone resorption	365:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	1	9	theme	calcium	174:180	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	1	10	theme	bone	277:280	arg1	repair					282:287	osteoporotic bone repair	264:287	osteoporotic bone repair	264:287	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	6	11	theme	release	1038:1044	arg1	effect					1046:1051	a sustained release effect	1026:1051	a sustained release effect	1026:1051	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	1	12	from	stimulation	316:326	arg1	resorption					370:379	bone resorption	365:379	bone resorption	365:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	0	13	theme	defect	117:122	arg1	repair					124:129	bone defect repair	112:129	bone defect repair in osteoporosis rats	112:150	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	1	14	theme	bone	365:368	arg1	resorption					370:379	bone resorption	365:379	bone resorption	365:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	8	15	dep	osteogenesis	1223:1234	arg1	inhibition					1270:1279	metabolism inhibition	1259:1279	metabolism inhibition	1259:1279	The in vivo results reveal that CS-SrCSH exhibits a long-term capability for osteogenesis, angiogenesis and bone metabolism inhibition.					
32580171	3	16	theme	Sr2+	544:547	arg1	utilization					529:539	the utilization	525:539	the utilization of Sr2+	525:547	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	5	17	theme	particle	832:839	arg1	size					841:844	uniform particle size	824:844	uniform particle size	824:844	It was observed that CS-SrCSH microspheres have uniform particle size.					
32580171	0	18	theme	osteoporosis	134:145	arg1	rats					147:150	osteoporosis rats	134:150	osteoporosis rats	134:150	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	6	19	theme	Sr2+	1000:1003	arg1	rate					992:995	the release rate	980:995	the release rate of Sr2+	980:1003	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	6	19	theme	Sr2+	1000:1003	arg1	stable					1008:1013	stable	1008:1013	stable	1008:1013	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	1	20	theme	sulfate	182:188	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	6	21	dep	in	881:882	arg1	vitro					884:888	vitro	884:888	vitro	884:888	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	1	22	from	reduction	352:360	arg1	resorption					370:379	bone resorption	365:379	bone resorption	365:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	2	23	theme	clinical	449:456	arg1	value					458:462	its clinical value	445:462	its clinical value	445:462	However, the rapid degradation and absorption of SrCSH affects its clinical value.					
32580171	6	24	theme	sustained	1028:1036	arg1	effect					1046:1051	a sustained release effect	1026:1051	a sustained release effect	1026:1051	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	1	25	theme	hemihydrate	190:200	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	7	26	theme	rat	1126:1128	arg1	defects					1137:1143	critical-sized OVX rat tibial defects	1107:1143	critical-sized OVX rat tibial defects	1107:1143	Furthermore, CS-SrCSH-based cement is used to repair critical-sized OVX rat tibial defects.					
32580171	9	27	dep	CS-SrCSH-based	1329:1342	arg1	cements					1344:1350	cements	1344:1350	cements	1344:1350	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	5	28	contain	have	819:822	arg2	size					841:844	uniform particle size	824:844	uniform particle size	824:844	It was observed that CS-SrCSH microspheres have uniform particle size.					
32580171	5	28	contain	have	819:822	arg1	microspheres					806:817	CS-SrCSH microspheres	797:817	CS-SrCSH microspheres	797:817	It was observed that CS-SrCSH microspheres have uniform particle size.					
32580171	8	29	theme	long-term	1198:1206	arg1	capability					1208:1217	a long-term capability	1196:1217	a long-term capability for osteogenesis, angiogenesis and bone metabolism inhibition	1196:1279	The in vivo results reveal that CS-SrCSH exhibits a long-term capability for osteogenesis, angiogenesis and bone metabolism inhibition.					
32580171	0	30	theme	Controlled	0:9	arg1	degradation					11:21	Controlled degradation	0:21	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	0:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	6	31	theme	degradation	890:900	arg1	time					902:905	the in vivo and in vitro degradation time	865:905	the in vivo and in vitro degradation time of CS-SrCSH microspheres	865:930	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	6	31	theme	degradation	890:900	arg1	longer					949:954	longer	949:954	longer	949:954	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	1	32	theme	SrCSH	203:207	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	0	33	theme	chitosan-coated	26:40	arg1	cement					96:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	9	34	from	repair	1395:1400	arg1	bone					1450:1453	the osteoporotic bone	1433:1453	the osteoporotic bone	1433:1453	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	9	35	theme	bone	1405:1408	arg1	defects					1410:1416	bone defects	1405:1416	bone defects	1405:1416	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	2	36	theme	SrCSH	431:435	arg1	absorption					417:426	absorption	417:426	absorption	417:426	However, the rapid degradation and absorption of SrCSH affects its clinical value.					
32580171	2	36	theme	SrCSH	431:435	arg1	degradation					401:411	rapid degradation	395:411	rapid degradation	395:411	However, the rapid degradation and absorption of SrCSH affects its clinical value.					
32580171	1	37	from	resorption	370:379	arg1	stimulation					316:326	the stimulation	312:326	the stimulation of bone formation and a reduction in bone resorption	312:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	9	38	theme	controllable	1301:1312	arg1	degradation					1314:1324	the controllable degradation	1297:1324	the controllable degradation of CS-SrCSH-based cements described here	1297:1365	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	9	38	theme	controllable	1301:1312	arg1	beneficial					1376:1385	beneficial	1376:1385	beneficial	1376:1385	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	3	39	theme	electrostatic	617:629	arg1	interaction					631:641	electrostatic interaction	617:641	electrostatic interaction between CS and SrCSH	617:662	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	3	40	theme	SrCSH	507:511	arg1	time					499:502	the degradation time	483:502	the degradation time of SrCSH	483:511	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	1	41	theme	bioactive	210:218	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	0	42	theme	calcium	58:64	arg1	cement					96:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	6	43	theme	microspheres	919:930	arg1	time					902:905	the in vivo and in vitro degradation time	865:905	the in vivo and in vitro degradation time of CS-SrCSH microspheres	865:930	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	6	43	theme	microspheres	919:930	arg1	longer					949:954	longer	949:954	longer	949:954	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	7	44	theme	tibial	1130:1135	arg1	defects					1137:1143	critical-sized OVX rat tibial defects	1107:1143	critical-sized OVX rat tibial defects	1107:1143	Furthermore, CS-SrCSH-based cement is used to repair critical-sized OVX rat tibial defects.					
32580171	3	45	theme	SrCSH	571:575	arg1	CS-SrCSH					591:598	CS-SrCSH	591:598	CS-SrCSH	591:598	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	3	45	theme	SrCSH	571:575	arg1	microspheres					577:588	chitosan (CS)-coated SrCSH microspheres	550:588	chitosan (CS)-coated SrCSH microspheres (CS-SrCSH)	550:599	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	8	46	dep	in	1150:1151	arg1	vivo					1153:1156	vivo	1153:1156	vivo	1153:1156	The in vivo results reveal that CS-SrCSH exhibits a long-term capability for osteogenesis, angiogenesis and bone metabolism inhibition.					
32580171	0	47	theme	strontium-doped	42:56	arg1	cement					96:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	6	48	theme	CS-SrCSH	910:917	arg1	microspheres					919:930	CS-SrCSH microspheres	910:930	CS-SrCSH microspheres	910:930	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	7	49	theme	critical-sized	1107:1120	arg1	defects					1137:1143	critical-sized OVX rat tibial defects	1107:1143	critical-sized OVX rat tibial defects	1107:1143	Furthermore, CS-SrCSH-based cement is used to repair critical-sized OVX rat tibial defects.					
32580171	5	50	theme	uniform	824:830	arg1	size					841:844	uniform particle size	824:844	uniform particle size	824:844	It was observed that CS-SrCSH microspheres have uniform particle size.					
32580171	0	51	theme	hemihydrate	74:84	arg1	cement					96:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	9	52	theme	defects	1410:1416	arg1	repair					1395:1400	the repair	1391:1400	the repair of bone defects	1391:1416	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	7	53	used	used	1092:1095	arg2	cement					1082:1087	CS-SrCSH-based cement	1067:1087	CS-SrCSH-based cement	1067:1087	Furthermore, CS-SrCSH-based cement is used to repair critical-sized OVX rat tibial defects.					
32580171	9	54	theme	osteoporotic	1437:1448	arg1	bone					1450:1453	the osteoporotic bone	1433:1453	the osteoporotic bone	1433:1453	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	3	55	theme	degradation	487:497	arg1	time					499:502	the degradation time	483:502	the degradation time of SrCSH	483:511	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	0	56	theme	sulfate	66:72	arg1	cement					96:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	1	57	theme	bone	331:334	arg1	formation					336:344	bone formation	331:344	bone formation	331:344	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	9	58	from	beneficial	1376:1385	arg1	conclusion					1285:1294	conclusion	1285:1294	conclusion	1285:1294	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	3	59	theme	-coated	563:569	arg1	CS-SrCSH					591:598	CS-SrCSH	591:598	CS-SrCSH	591:598	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	3	59	theme	-coated	563:569	arg1	microspheres					577:588	chitosan (CS)-coated SrCSH microspheres	550:588	chitosan (CS)-coated SrCSH microspheres (CS-SrCSH)	550:599	In order to delay the degradation time of SrCSH and improve the utilization of Sr2+, chitosan (CS)-coated SrCSH microspheres (CS-SrCSH) are prepared by electrostatic interaction between CS and SrCSH.					
32580171	1	60	theme	formation	336:344	arg1	stimulation					316:326	the stimulation	312:326	the stimulation of bone formation and a reduction in bone resorption	312:379	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	1	61	theme	Strontium	153:161	arg1	materials					220:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials	153:228	Strontium (Sr)-doped calcium sulfate hemihydrate (SrCSH) bioactive materials have been demonstrated to promote osteoporotic bone repair, being associated with the stimulation of bone formation and a reduction in bone resorption.					
32580171	4	62	theme	diffraction	671:681	arg1	analysis					683:690	X-ray diffraction analysis	665:690	X-ray diffraction analysis	665:690	X-ray diffraction analysis verifies that SrCSH coated by CS does not alter the phase composition of the SrCSH.					
32580171	6	63	theme	release	984:990	arg1	rate					992:995	the release rate	980:995	the release rate of Sr2+	980:1003	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	6	63	theme	release	984:990	arg1	stable					1008:1013	stable	1008:1013	stable	1008:1013	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	4	64	theme	phase	744:748	arg1	composition					750:760	the phase composition	740:760	the phase composition of the SrCSH	740:773	X-ray diffraction analysis verifies that SrCSH coated by CS does not alter the phase composition of the SrCSH.					
32580171	0	65	from	repair	124:129	arg1	rats					147:150	osteoporosis rats	134:150	osteoporosis rats	134:150	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	9	66	from	conclusion	1285:1294	arg1	beneficial					1376:1385	beneficial	1376:1385	beneficial	1376:1385	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	9	66	from	conclusion	1285:1294	arg1	degradation					1314:1324	the controllable degradation	1297:1324	the controllable degradation of CS-SrCSH-based cements described here	1297:1365	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	8	67	theme	metabolism	1259:1268	arg1	inhibition					1270:1279	metabolism inhibition	1259:1279	metabolism inhibition	1259:1279	The in vivo results reveal that CS-SrCSH exhibits a long-term capability for osteogenesis, angiogenesis and bone metabolism inhibition.					
32580171	0	68	theme	cement	96:101	arg1	degradation					11:21	Controlled degradation	0:21	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	0:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	2	69	dep	degradation	401:411	arg1	the					391:393	the	391:393	the	391:393	However, the rapid degradation and absorption of SrCSH affects its clinical value.					
32580171	5	70	theme	CS-SrCSH	797:804	arg1	microspheres					806:817	CS-SrCSH microspheres	797:817	CS-SrCSH microspheres	797:817	It was observed that CS-SrCSH microspheres have uniform particle size.					
32580171	4	71	theme	X-ray	665:669	arg1	analysis					683:690	X-ray diffraction analysis	665:690	X-ray diffraction analysis	665:690	X-ray diffraction analysis verifies that SrCSH coated by CS does not alter the phase composition of the SrCSH.					
32580171	2	72	theme	rapid	395:399	arg1	degradation					401:411	rapid degradation	395:411	rapid degradation	395:411	However, the rapid degradation and absorption of SrCSH affects its clinical value.					
32580171	6	73	dep	in	869:870	arg1	vivo					872:875	vivo	872:875	vivo	872:875	More importantly, the in vivo and in vitro degradation time of CS-SrCSH microspheres is significantly longer than that of SrCSH, and the release rate of Sr2+ is stable, achieving a sustained release effect.					
32580171	0	74	theme	composite	86:94	arg1	cement					96:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement	26:101	Controlled degradation of chitosan-coated strontium-doped calcium sulfate hemihydrate composite cement promotes bone defect repair in osteoporosis rats.					
32580171	4	75	theme	SrCSH	769:773	arg1	composition					750:760	the phase composition	740:760	the phase composition of the SrCSH	740:773	X-ray diffraction analysis verifies that SrCSH coated by CS does not alter the phase composition of the SrCSH.					
32580171	7	76	theme	OVX	1122:1124	arg1	defects					1137:1143	critical-sized OVX rat tibial defects	1107:1143	critical-sized OVX rat tibial defects	1107:1143	Furthermore, CS-SrCSH-based cement is used to repair critical-sized OVX rat tibial defects.					
32580171	9	77	theme	CS-SrCSH-based	1329:1342	arg1	degradation					1314:1324	the controllable degradation	1297:1324	the controllable degradation of CS-SrCSH-based cements described here	1297:1365	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32580171	9	77	theme	CS-SrCSH-based	1329:1342	arg1	beneficial					1376:1385	beneficial	1376:1385	beneficial	1376:1385	In conclusion, the controllable degradation of CS-SrCSH-based cements described here could be beneficial for the repair of bone defects, especially in the osteoporotic bone.					
32979942	5	0	theme	glycans	689:695	arg1	states					668:673	overall processing states	649:673	overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans	649:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	5	1	from	spikes	591:596	arg1	Compositions					538:549	Compositions	538:549	Compositions of the N-linked glycans from the native spikes	538:596	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	3	2	theme	authentic	313:321	arg1	virus					334:338	the authentic SARS-CoV-2 virus	309:338	the authentic SARS-CoV-2 virus	309:338	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	4	3	theme	average	505:511	arg1	resolutions					513:523	average resolutions	505:523	average resolutions of 8.7-11 Å	505:535	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	5	4	theme	overall	649:655	arg1	states					668:673	overall processing states	649:673	overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans	649:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	6	5	theme	ribonucleoproteins	792:809	arg1	conformation					772:783	The native conformation	761:783	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies	761:850	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed.					
32979942	4	6	theme	S	438:438	arg1	proteins					440:447	the S proteins	434:447	the S proteins in pre- and postfusion conformations	434:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	5	7	theme	processing	657:666	arg1	states					668:673	overall processing states	649:673	overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans	649:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	2	8	theme	structural	151:160	arg1	elucidation					162:172	the structural elucidation	147:172	the structural elucidation of SARS-CoV-2 proteins	147:195	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	5	9	theme	recombinant	727:737	arg1	glycans					752:758	the recombinant glycoprotein glycans	723:758	the recombinant glycoprotein glycans	723:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	4	10	theme	pre-	452:455	arg1	conformations					472:484	pre- and postfusion conformations	452:484	pre- and postfusion conformations	452:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	4	11	from	conformations	472:484	arg1	structures					420:429	Native structures	413:429	Native structures of the S proteins in pre- and postfusion conformations	413:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	1	12	theme	enveloped	65:73	arg1	virus					75:79	an enveloped virus	62:79	an enveloped virus responsible for the COVID-19 pandemic	62:117	SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic.					
32979942	1	12	theme	enveloped	65:73	arg1	SARS-CoV-2					48:57	SARS-CoV-2	48:57	SARS-CoV-2	48:57	SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic.					
32979942	7	13	from	RNA	1043:1045	arg1	lumen					1070:1074	the ∼80-nm-diameter lumen	1050:1074	the ∼80-nm-diameter lumen	1050:1074	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	5	14	theme	similar	704:710	arg1	glycans					689:695	the native glycans	678:695	the native glycans highly similar to that of the recombinant glycoprotein glycans	678:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	3	15	theme	cryoelectron	346:357	arg1	cryo-ET					371:377	cryo-ET	371:377	cryo-ET	371:377	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	3	15	theme	cryoelectron	346:357	arg1	tomography					359:368	cryoelectron tomography	346:368	cryoelectron tomography (cryo-ET)	346:378	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	5	16	theme	glycans	567:573	arg1	Compositions					538:549	Compositions	538:549	Compositions of the N-linked glycans from the native spikes	538:596	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	0	17	theme	Molecular	0:8	arg1	Architecture					10:21	Molecular Architecture	0:21	Molecular Architecture of the SARS-CoV-2 Virus	0:45	Molecular Architecture of the SARS-CoV-2 Virus.					
32979942	4	18	theme	postfusion	461:470	arg1	conformations					472:484	pre- and postfusion conformations	452:484	pre- and postfusion conformations	452:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	1	19	theme	responsible	81:91	arg1	virus					75:79	an enveloped virus	62:79	an enveloped virus responsible for the COVID-19 pandemic	62:117	SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic.					
32979942	1	19	theme	responsible	81:91	arg1	SARS-CoV-2					48:57	SARS-CoV-2	48:57	SARS-CoV-2	48:57	SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic.					
32979942	7	20	theme	exceptional	953:963	arg1	detail					965:970	exceptional detail	953:970	exceptional detail	953:970	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	3	21	theme	subtomogram	384:394	arg1	STA					407:409	STA	407:409	STA	407:409	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	3	21	theme	subtomogram	384:394	arg1	averaging					396:404	subtomogram averaging	384:404	subtomogram averaging (STA)	384:410	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	7	22	theme	virus	944:948	arg1	architecture					913:924	the architecture	909:924	the architecture of the SARS-CoV-2 virus	909:948	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	5	23	theme	mass	615:618	arg1	spectrometry					620:631	mass spectrometry	615:631	mass spectrometry	615:631	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	5	24	theme	native	584:589	arg1	spikes					591:596	the native spikes	580:596	the native spikes	580:596	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	5	25	gly	glycoprotein	739:750	arg1	glycoprotein					739:750	the recombinant glycoprotein glycans	723:758	the recombinant glycoprotein glycans	723:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	4	26	from	proteins	440:447	arg1	conformations					472:484	pre- and postfusion conformations	452:484	pre- and postfusion conformations	452:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	2	27	theme	proteins	188:195	arg1	elucidation					162:172	the structural elucidation	147:172	the structural elucidation of SARS-CoV-2 proteins	147:195	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	1	28	theme	COVID-19	101:108	arg1	pandemic					110:117	the COVID-19 pandemic	97:117	the COVID-19 pandemic	97:117	SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic.					
32979942	0	29	theme	Virus	41:45	arg1	Architecture					10:21	Molecular Architecture	0:21	Molecular Architecture of the SARS-CoV-2 Virus	0:45	Molecular Architecture of the SARS-CoV-2 Virus.					
32979942	2	30	theme	SARS-CoV-2	177:186	arg1	proteins					188:195	SARS-CoV-2 proteins	177:195	SARS-CoV-2 proteins	177:195	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	2	31	theme	virus	238:242	arg1	architecture					211:222	the detailed architecture	198:222	the detailed architecture of the intact virus	198:242	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	0	32	theme	SARS-CoV-2	30:39	arg1	Virus					41:45	the SARS-CoV-2 Virus	26:45	the SARS-CoV-2 Virus	26:45	Molecular Architecture of the SARS-CoV-2 Virus.					
32979942	2	33	from	advances	135:142	arg1	elucidation					162:172	the structural elucidation	147:172	the structural elucidation of SARS-CoV-2 proteins	147:195	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	2	34	theme	intact	231:236	arg1	virus					238:242	the intact virus	227:242	the intact virus	227:242	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	6	35	theme	native	765:770	arg1	conformation					772:783	The native conformation	761:783	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies	761:850	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed.					
32979942	5	36	theme	glycoprotein	739:750	arg1	glycans					752:758	the recombinant glycoprotein glycans	723:758	the recombinant glycoprotein glycans	723:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	5	37	link	N-linked	558:565	arg1	glycans					567:573	the N-linked glycans	554:573	the N-linked glycans	554:573	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	7	38	theme	∼80-nm-diameter	1054:1068	arg1	lumen					1070:1074	the ∼80-nm-diameter lumen	1050:1074	the ∼80-nm-diameter lumen	1050:1074	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	4	39	theme	proteins	440:447	arg1	structures					420:429	Native structures	413:429	Native structures of the S proteins in pre- and postfusion conformations	413:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	4	40	from	structures	420:429	arg1	conformations					472:484	pre- and postfusion conformations	452:484	pre- and postfusion conformations	452:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	4	41	theme	Native	413:418	arg1	structures					420:429	Native structures	413:429	Native structures of the S proteins in pre- and postfusion conformations	413:484	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	6	42	theme	assemblies	841:850	arg1	conformation					772:783	The native conformation	761:783	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies	761:850	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed.					
32979942	4	43	theme	8.7-11 Å	528:535	arg1	resolutions					513:523	average resolutions	505:523	average resolutions of 8.7-11 Å	505:535	Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å.					
32979942	5	44	theme	native	682:687	arg1	glycans					689:695	the native glycans	678:695	the native glycans highly similar to that of the recombinant glycoprotein glycans	678:758	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	2	45	theme	detailed	202:209	arg1	architecture					211:222	the detailed architecture	198:222	the detailed architecture of the intact virus	198:242	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
32979942	5	46	theme	N-linked	558:565	arg1	glycans					567:573	the N-linked glycans	554:573	the N-linked glycans	554:573	Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans.					
32979942	7	47	theme	SARS-CoV-2	933:942	arg1	virus					944:948	the SARS-CoV-2 virus	929:948	the SARS-CoV-2 virus	929:948	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	3	48	theme	molecular	287:295	arg1	assembly					297:304	the molecular assembly	283:304	the molecular assembly of the authentic SARS-CoV-2 virus	283:338	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	6	49	theme	higher-order	828:839	arg1	assemblies					841:850	their higher-order assemblies	822:850	their higher-order assemblies	822:850	The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed.					
32979942	7	50	theme	single-segmented	1026:1041	arg1	RNA					1043:1045	its ∼30-kb-long single-segmented RNA	1010:1045	its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen	1010:1074	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	3	51	theme	SARS-CoV-2	323:332	arg1	virus					334:338	the authentic SARS-CoV-2 virus	309:338	the authentic SARS-CoV-2 virus	309:338	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	7	52	theme	∼30-kb-long	1014:1024	arg1	RNA					1043:1045	its ∼30-kb-long single-segmented RNA	1010:1045	its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen	1010:1074	Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.					
32979942	3	53	theme	virus	334:338	arg1	assembly					297:304	the molecular assembly	283:304	the molecular assembly of the authentic SARS-CoV-2 virus	283:338	Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA).					
32979942	2	54	theme	recent	128:133	arg1	advances					135:142	recent advances	128:142	recent advances in the structural elucidation of SARS-CoV-2 proteins	128:195	Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled.					
34884890	0	0	theme	Plantago	78:85	arg1	Exudates					66:73	Phloem Exudates	59:73	Phloem Exudates of Plantago major and Poa annua	59:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	7	1	from	plants	1044:1049	arg1	proportions					997:1007	proportions	997:1007	proportions of some amino acids in mycorrhizal plants	997:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	7	2	theme	acids	1023:1027	arg1	proportions					997:1007	proportions	997:1007	proportions of some amino acids in mycorrhizal plants	997:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	1	3	theme	Arbuscular	134:143	arg1	AM					157:158	AM	157:158	AM	157:158	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	1	3	theme	Arbuscular	134:143	arg1	mycorrhiza					145:154	Arbuscular mycorrhiza	134:154	Arbuscular mycorrhiza (AM)	134:159	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	1	4	theme	mycorrhizal	210:220	arg1	fungi					222:226	arbuscular mycorrhizal fungi	199:226	arbuscular mycorrhizal fungi (AMF)	199:232	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	1	4	theme	mycorrhizal	210:220	arg1	AMF					229:231	AMF	229:231	AMF	229:231	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	7	5	from	changes	1061:1067	arg1	patterns					1110:1117	phloem upload and resource allocation patterns	1072:1117	phloem upload and resource allocation patterns within the plants	1072:1135	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	7	6	from	shifts	987:992	arg1	proportions					997:1007	proportions	997:1007	proportions of some amino acids in mycorrhizal plants	997:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	9	7	theme	subsequent	1321:1330	arg1	generation					1332:1341	a subsequent generation	1319:1341	a subsequent generation	1319:1341	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	3	8	theme	phloem	436:441	arg1	exudates					443:450	phloem exudates	436:450	phloem exudates of the plant species Plantago major and Poa annua	436:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	6	9	theme	metabolic	898:906	arg1	effects					908:914	metabolic effects	898:914	metabolic effects of AM	898:920	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	7	10	theme	higher	953:958	arg1	proportions					960:970	Slightly higher proportions	944:970	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants	944:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	0	11	from	Effects	0:6	arg1	Metabolites					44:54	Primary Metabolites	36:54	Primary Metabolites in Phloem Exudates of Plantago major and Poa annua	36:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	0	11	from	Effects	0:6	arg1	Aphid					127:131	a Generalist Aphid	114:131	a Generalist Aphid	114:131	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	9	12	dep	affected	1217:1224	arg1	whereas					1262:1268	whereas	1262:1268	whereas	1262:1268	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	3	13	theme	species	465:471	arg1	exudates					443:450	phloem exudates	436:450	phloem exudates of the plant species Plantago major and Poa annua	436:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	7	14	from	sucrose	975:981	arg1	proportions					997:1007	proportions	997:1007	proportions of some amino acids in mycorrhizal plants	997:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	0	15	theme	major	87:91	arg1	Plantago					78:85	Plantago major	78:91	Plantago major	78:91	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	7	16	theme	amino	1017:1021	arg1	acids					1023:1027	some amino acids	1012:1027	some amino acids in mycorrhizal plants	1012:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	4	17	theme	phloem	600:605	arg1	exudates					607:614	leaf phloem exudates	595:614	leaf phloem exudates	595:614	Plants were grown without or with a generalist AMF species, leaf phloem exudates were collected, and primary metabolites were measured.					
34884890	9	18	theme	P.	1307:1308	arg1	annua					1310:1314	P. annua	1307:1314	P. annua	1307:1314	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	0	19	theme	annua	101:105	arg1	Exudates					66:73	Phloem Exudates	59:73	Phloem Exudates of Plantago major and Poa annua	59:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	0	20	from	Metabolites	44:54	arg1	Exudates					66:73	Phloem Exudates	59:73	Phloem Exudates of Plantago major and Poa annua	59:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	4	21	theme	primary	636:642	arg1	metabolites					644:654	primary metabolites	636:654	primary metabolites	636:654	Plants were grown without or with a generalist AMF species, leaf phloem exudates were collected, and primary metabolites were measured.					
34884890	4	22	theme	AMF	582:584	arg1	species					586:592	a generalist AMF species	569:592	a generalist AMF species	569:592	Plants were grown without or with a generalist AMF species, leaf phloem exudates were collected, and primary metabolites were measured.					
34884890	3	23	dep	species	465:471	arg1	species					465:471	the plant species Plantago major and Poa annua	455:500	the plant species Plantago major and Poa annua	455:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	23	dep	species	465:471	arg1	Plantago					473:480	Plantago major	473:486	Plantago major	473:486	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	23	dep	species	465:471	arg1	annua					496:500	Poa annua	492:500	Poa annua	492:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	0	24	theme	Poa	97:99	arg1	annua					101:105	Poa annua	97:105	Poa annua	97:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	7	25	from	proportions	960:970	arg1	proportions					997:1007	proportions	997:1007	proportions of some amino acids in mycorrhizal plants	997:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	9	26	located	found	1298:1302	arg1	annua					1310:1314	P. annua	1307:1314	P. annua	1307:1314	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	9	26	located	found	1298:1302	arg1	generation					1332:1341	a subsequent generation	1319:1341	a subsequent generation	1319:1341	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	9	26	located	found	1298:1302	arg2	effects					1279:1285	positive effects	1270:1285	positive effects of AM	1270:1291	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	3	27	theme	plant	459:463	arg1	species					465:471	the plant species Plantago major and Poa annua	455:500	the plant species Plantago major and Poa annua	455:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	27	theme	plant	459:463	arg1	Plantago					473:480	Plantago major	473:486	Plantago major	473:486	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	27	theme	plant	459:463	arg1	annua					496:500	Poa annua	492:500	Poa annua	492:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	7	28	theme	resource	1090:1097	arg1	allocation					1099:1108	resource allocation	1090:1108	resource allocation	1090:1108	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	5	29	theme	persicae	707:714	arg1	performance					689:699	the performance	685:699	the performance of M. persicae on control and mycorrhizal plants of both species	685:764	Additionally, the performance of M. persicae on control and mycorrhizal plants of both species was assessed.					
34884890	9	30	dep	major	1255:1259	arg1	P.					1252:1253	P. major	1252:1259	P. major	1252:1259	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	7	31	theme	shifts	987:992	arg1	proportions					960:970	Slightly higher proportions	944:970	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants	944:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	2	32	from	Effects	294:300	arg1	composition					325:335	the metabolic composition	311:335	the metabolic composition of plant phloem sap	311:355	Effects of AM on the metabolic composition of plant phloem sap may affect aphids.					
34884890	3	33	theme	Poa	492:494	arg1	species					465:471	the plant species Plantago major and Poa annua	455:500	the plant species Plantago major and Poa annua	455:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	33	theme	Poa	492:494	arg1	annua					496:500	Poa annua	492:500	Poa annua	492:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	6	34	theme	plant	790:794	arg1	species					796:802	the plant species	786:802	the plant species	786:802	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	2	35	theme	metabolic	315:323	arg1	composition					325:335	the metabolic composition	311:335	the metabolic composition of plant phloem sap	311:355	Effects of AM on the metabolic composition of plant phloem sap may affect aphids.					
34884890	6	36	theme	phloem	881:886	arg1	exudates					888:895	their phloem exudates	875:895	their phloem exudates	875:895	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	4	37	theme	generalist	571:580	arg1	species					586:592	a generalist AMF species	569:592	a generalist AMF species	569:592	Plants were grown without or with a generalist AMF species, leaf phloem exudates were collected, and primary metabolites were measured.					
34884890	0	38	theme	Mycorrhiza	22:31	arg1	Effects					0:6	Effects	0:6	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.	0:132	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	10	39	theme	other	1352:1356	arg1	factors					1358:1364	other factors	1352:1364	other factors	1352:1364	Next to other factors, the metabolic composition of the phloem exudates may partly explain these findings.					
34884890	7	40	theme	sucrose	975:981	arg1	proportions					960:970	Slightly higher proportions	944:970	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants	944:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	3	41	from	metabolites	421:431	arg1	exudates					443:450	phloem exudates	436:450	phloem exudates of the plant species Plantago major and Poa annua	436:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	42	theme	major	482:486	arg1	species					465:471	the plant species Plantago major and Poa annua	455:500	the plant species Plantago major and Poa annua	455:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	42	theme	major	482:486	arg1	Plantago					473:480	Plantago major	473:486	Plantago major	473:486	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	5	43	theme	mycorrhizal	731:741	arg1	plants					743:748	control and mycorrhizal plants	719:748	control and mycorrhizal plants of both species	719:764	Additionally, the performance of M. persicae on control and mycorrhizal plants of both species was assessed.					
34884890	0	44	theme	Arbuscular	11:20	arg1	Mycorrhiza					22:31	Arbuscular Mycorrhiza	11:31	Arbuscular Mycorrhiza	11:31	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	7	45	from	proportions	997:1007	arg1	plants					1044:1049	mycorrhizal plants	1032:1049	mycorrhizal plants	1032:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	7	45	from	proportions	997:1007	arg1	proportions					960:970	Slightly higher proportions	944:970	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants	944:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	1	46	with	interaction	172:182	arg1	fungi					222:226	arbuscular mycorrhizal fungi	199:226	arbuscular mycorrhizal fungi (AMF)	199:232	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	1	46	with	interaction	172:182	arg1	AMF					229:231	AMF	229:231	AMF	229:231	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	4	47	theme	leaf	595:598	arg1	exudates					607:614	leaf phloem exudates	595:614	leaf phloem exudates	595:614	Plants were grown without or with a generalist AMF species, leaf phloem exudates were collected, and primary metabolites were measured.					
34884890	2	48	theme	sap	353:355	arg1	composition					325:335	the metabolic composition	311:335	the metabolic composition of plant phloem sap	311:355	Effects of AM on the metabolic composition of plant phloem sap may affect aphids.					
34884890	0	49	theme	Primary	36:42	arg1	Metabolites					44:54	Primary Metabolites	36:54	Primary Metabolites in Phloem Exudates of Plantago major and Poa annua	36:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	6	50	theme	metabolites	860:870	arg1	proportions					837:847	the relative proportions	824:847	the relative proportions of primary metabolites in their phloem exudates	824:895	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	0	51	theme	Generalist	116:125	arg1	Aphid					127:131	a Generalist Aphid	114:131	a Generalist Aphid	114:131	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	8	52	theme	higher	1154:1159	arg1	performance					1161:1171	a higher performance	1152:1171	a higher performance on P. annua	1152:1183	Aphids showed a higher performance on P. annua than on P. major.					
34884890	2	53	theme	phloem	346:351	arg1	sap					353:355	plant phloem sap	340:355	plant phloem sap	340:355	Effects of AM on the metabolic composition of plant phloem sap may affect aphids.					
34884890	5	54	theme	species	758:764	arg1	plants					743:748	control and mycorrhizal plants	719:748	control and mycorrhizal plants of both species	719:764	Additionally, the performance of M. persicae on control and mycorrhizal plants of both species was assessed.					
34884890	1	55	theme	plant	252:256	arg1	growth					258:263	plant growth	252:263	plant growth	252:263	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	9	56	from	survival	1230:1237	arg1	major					1255:1259	major	1255:1259	major	1255:1259	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	5	57	theme	control	719:725	arg1	plants					743:748	control and mycorrhizal plants	719:748	control and mycorrhizal plants of both species	719:764	Additionally, the performance of M. persicae on control and mycorrhizal plants of both species was assessed.					
34884890	8	58	from	performance	1161:1171	arg1	annua					1179:1183	P. annua	1176:1183	P. annua	1176:1183	Aphids showed a higher performance on P. annua than on P. major.					
34884890	2	59	theme	plant	340:344	arg1	sap					353:355	plant phloem sap	340:355	plant phloem sap	340:355	Effects of AM on the metabolic composition of plant phloem sap may affect aphids.					
34884890	7	60	theme	mycorrhizal	1032:1042	arg1	plants					1044:1049	mycorrhizal plants	1032:1049	mycorrhizal plants	1032:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	6	61	theme	primary	852:858	arg1	metabolites					860:870	primary metabolites	852:870	primary metabolites	852:870	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	6	62	from	proportions	837:847	arg1	exudates					888:895	their phloem exudates	875:895	their phloem exudates	875:895	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	3	63	theme	primary	413:419	arg1	metabolites					421:431	primary metabolites	413:431	primary metabolites in phloem exudates of the plant species Plantago major and Poa annua	413:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	5	64	from	performance	689:699	arg1	plants					743:748	control and mycorrhizal plants	719:748	control and mycorrhizal plants of both species	719:764	Additionally, the performance of M. persicae on control and mycorrhizal plants of both species was assessed.					
34884890	7	65	from	acids	1023:1027	arg1	plants					1044:1049	mycorrhizal plants	1032:1049	mycorrhizal plants	1032:1049	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	7	66	theme	allocation	1099:1108	arg1	patterns					1110:1117	phloem upload and resource allocation patterns	1072:1117	phloem upload and resource allocation patterns within the plants	1072:1135	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	10	67	theme	exudates	1407:1414	arg1	composition					1381:1391	the metabolic composition	1367:1391	the metabolic composition of the phloem exudates	1367:1414	Next to other factors, the metabolic composition of the phloem exudates may partly explain these findings.					
34884890	3	68	from	impacts	396:402	arg1	aphid					513:517	the aphid	509:517	the aphid Myzus persicae	509:532	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	3	68	from	impacts	396:402	arg1	metabolites					421:431	primary metabolites	413:431	primary metabolites in phloem exudates of the plant species Plantago major and Poa annua	413:500	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	6	69	theme	relative	828:835	arg1	proportions					837:847	the relative proportions	824:847	the relative proportions of primary metabolites in their phloem exudates	824:895	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	9	70	theme	aphids	1242:1247	arg1	survival					1230:1237	the survival	1226:1237	the survival of aphids on P. major	1226:1259	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	3	71	theme	AM	407:408	arg1	impacts					396:402	the impacts	392:402	the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae	392:532	We investigated the impacts of AM on primary metabolites in phloem exudates of the plant species Plantago major and Poa annua and on the aphid Myzus persicae.					
34884890	9	72	theme	positive	1270:1277	arg1	effects					1279:1285	positive effects	1270:1285	positive effects of AM	1270:1291	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	10	73	theme	metabolic	1371:1379	arg1	composition					1381:1391	the metabolic composition	1367:1391	the metabolic composition of the phloem exudates	1367:1414	Next to other factors, the metabolic composition of the phloem exudates may partly explain these findings.					
34884890	7	74	theme	upload	1079:1084	arg1	patterns					1110:1117	phloem upload and resource allocation patterns	1072:1117	phloem upload and resource allocation patterns within the plants	1072:1135	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	8	75	dep	major	1196:1200	arg1	P.					1193:1194	P. major	1193:1200	P. major	1193:1200	Aphids showed a higher performance on P. annua than on P. major.					
34884890	1	76	theme	plants	187:192	arg1	interaction					172:182	the interaction	168:182	the interaction	168:182	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	8	77	theme	P.	1176:1177	arg1	annua					1179:1183	P. annua	1176:1183	P. annua	1176:1183	Aphids showed a higher performance on P. annua than on P. major.					
34884890	10	78	theme	phloem	1400:1405	arg1	exudates					1407:1414	the phloem exudates	1396:1414	the phloem exudates	1396:1414	Next to other factors, the metabolic composition of the phloem exudates may partly explain these findings.					
34884890	6	79	theme	AM	919:920	arg1	effects					908:914	metabolic effects	898:914	metabolic effects of AM	898:920	While the plant species differed largely in the relative proportions of primary metabolites in their phloem exudates, metabolic effects of AM were less pronounced.					
34884890	0	80	theme	Phloem	59:64	arg1	Exudates					66:73	Phloem Exudates	59:73	Phloem Exudates of Plantago major and Poa annua	59:105	Effects of Arbuscular Mycorrhiza on Primary Metabolites in Phloem Exudates of Plantago major and Poa annua and on a Generalist Aphid.					
34884890	7	81	theme	phloem	1072:1077	arg1	upload					1079:1084	phloem upload	1072:1084	phloem upload	1072:1084	Slightly higher proportions of sucrose and shifts in proportions of some amino acids in mycorrhizal plants indicated changes in phloem upload and resource allocation patterns within the plants.					
34884890	2	82	theme	AM	305:306	arg1	Effects					294:300	Effects	294:300	Effects of AM on the metabolic composition of plant phloem sap	294:355	Effects of AM on the metabolic composition of plant phloem sap may affect aphids.					
34884890	9	83	theme	AM	1290:1291	arg1	effects					1279:1285	positive effects	1270:1285	positive effects of AM	1270:1291	AM negatively affected the survival of aphids on P. major, whereas positive effects of AM were found on P. annua in a subsequent generation.					
34884890	1	84	theme	arbuscular	199:208	arg1	fungi					222:226	arbuscular mycorrhizal fungi	199:226	arbuscular mycorrhizal fungi (AMF)	199:232	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34884890	1	84	theme	arbuscular	199:208	arg1	AMF					229:231	AMF	229:231	AMF	229:231	Arbuscular mycorrhiza (AM), i.e., the interaction of plants with arbuscular mycorrhizal fungi (AMF), often influences plant growth, physiology, and metabolism.					
34087583	14	0	theme	greater	2484:2490	arg1	retention					2502:2510	markedly greater ubiquinol retention	2475:2510	markedly greater ubiquinol retention	2475:2510	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	10	1	theme	complexed	1757:1765	arg1	ubiquinol					1767:1775	complexed ubiquinol	1757:1775	complexed ubiquinol	1757:1775	Furthermore, complexed ubiquinol was more effective at lowering ROS, and at far lower concentrations, compared to free ubiquinol.					
34087583	14	2	theme	ubiquinol	2492:2500	arg1	retention					2502:2510	markedly greater ubiquinol retention	2475:2510	markedly greater ubiquinol retention	2475:2510	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	16	3	theme	eye	3032:3034	arg1	drops					3036:3040	topical eye drops	3024:3040	topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	3024:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	2	4	theme	reactive	310:317	arg1	ROS					335:337	ROS	335:337	ROS	335:337	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	2	4	theme	reactive	310:317	arg1	species					326:332	reactive oxygen species	310:332	accumulated reactive oxygen species (ROS) inside cells	298:351	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	13	5	theme	porcine	2254:2260	arg1	corneas					2262:2268	porcine corneas	2254:2268	porcine corneas	2254:2268	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	1	6	theme	increased	123:131	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	6	theme	increased	123:131	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	6	theme	increased	123:131	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	6	theme	increased	123:131	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	7	7	contain	has	1174:1176	arg2	affinity					1200:1207	the strongest binding affinity	1178:1207	the strongest binding affinity with ubiquinol compared to α- or β-CD	1178:1245	Docking studies showed that γ-CD has the strongest binding affinity with ubiquinol compared to α- or β-CD.					
34087583	7	7	contain	has	1174:1176	arg1	γ-CD					1169:1172	γ-CD	1169:1172	γ-CD	1169:1172	Docking studies showed that γ-CD has the strongest binding affinity with ubiquinol compared to α- or β-CD.					
34087583	3	8	theme	major	527:531	arg1	reason					533:538	a major reason	525:538	a major reason behind cell death prior to surgery and decreased graft survival after transplantation	525:624	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	3	8	theme	major	527:531	arg1	stress					364:369	Oxidative stress	354:369	Oxidative stress	354:369	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	15	9	theme	higher	2732:2737	arg1	amounts					2739:2745	higher amounts	2732:2745	higher amounts of ubiquinol	2732:2758	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	15	9	theme	higher	2732:2737	arg1	ubiquinol					2750:2758	ubiquinol	2750:2758	ubiquinol	2750:2758	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	1	10	theme	donor	185:189	arg1	tissues					191:197	donor tissues	185:197	donor tissues	185:197	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	15	11	from	days	2662:2665	arg1	Optisol-GS®					2670:2680	Optisol-GS®	2670:2680	Optisol-GS® per standard industry practices	2670:2712	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	16	12	theme	products	2954:2961	arg1	variety					2929:2935	a variety	2927:2935	a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	2927:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	16	12	theme	products	2954:2961	arg1	products					2954:2961	aqueous-phase products	2940:2961	aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	2940:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	2	13	theme	species	326:332	arg1	cells					347:351	accumulated reactive oxygen species (ROS) inside cells	298:351	accumulated reactive oxygen species (ROS) inside cells	298:351	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	16	14	from	composition	2885:2895	arg1	summary					2827:2833	summary	2827:2833	summary	2827:2833	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	8	15	theme	Refresh®	1483:1490	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	3	16	theme	graft	589:593	arg1	survival					595:602	decreased graft survival	579:602	decreased graft survival	579:602	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	9	17	theme	ROS	1527:1529	arg1	activity					1542:1549	Greater ROS scavenging activity	1519:1549	Greater ROS scavenging activity	1519:1549	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	8	18	theme	tear	1503:1506	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	4	19	theme	coenzyme	703:710	arg1	Q10					712:714	coenzyme Q10	703:714	coenzyme Q10	703:714	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	16	20	theme	donor	2992:2996	arg1	media					3014:3018	donor corneal storage media	2992:3018	donor corneal storage media	2992:3018	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	10	21	theme	lower	1824:1828	arg1	concentrations					1830:1843	far lower concentrations	1820:1843	far lower concentrations	1820:1843	Furthermore, complexed ubiquinol was more effective at lowering ROS, and at far lower concentrations, compared to free ubiquinol.					
34087583	1	22	theme	cell	133:136	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	22	theme	cell	133:136	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	22	theme	cell	133:136	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	22	theme	cell	133:136	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	16	23	theme	storage	3006:3012	arg1	media					3014:3018	donor corneal storage media	2992:3018	donor corneal storage media	2992:3018	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	13	24	theme	lipophilic	2186:2195	arg1	coumarin-6					2202:2211	coumarin-6	2202:2211	coumarin-6	2202:2211	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	13	24	theme	lipophilic	2186:2195	arg1	dye					2197:2199	a lipophilic dye	2184:2199	a lipophilic dye (coumarin-6)	2184:2212	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	5	25	theme	aqueous-based	925:937	arg1	formulation					939:949	an aqueous-based formulation	922:949	an aqueous-based formulation of this molecule	922:966	However, ubiquinol is highly lipophilic, underscoring the need for an aqueous-based formulation of this molecule.					
34087583	8	26	theme	different	1336:1344	arg1	medium					1412:1417	Optisol-GS® corneal storage medium	1384:1417	Optisol-GS® corneal storage medium	1384:1417	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	26	theme	different	1336:1344	arg1	products					1365:1372	different aqueous ophthalmic products	1336:1372	different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops	1336:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	26	theme	different	1336:1344	arg1	solution					1434:1441	balanced salt solution	1420:1441	balanced salt solution for intraocular irrigation	1420:1468	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	26	theme	different	1336:1344	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	15	27	theme	free	2808:2811	arg1	ubiquinol					2813:2821	free ubiquinol	2808:2821	free ubiquinol	2808:2821	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	13	28	theme	inclusion	2154:2162	arg1	complex					2164:2170	a topically applied inclusion complex	2134:2170	a topically applied inclusion complex of γ-CD	2134:2178	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	13	29	theme	corneal	2331:2337	arg1	endothelium					2339:2349	the corneal endothelium	2327:2349	the corneal endothelium	2327:2349	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	6	30	theme	stable	1012:1017	arg1	formulation					1019:1029	a highly dispersible and stable formulation	987:1029	a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products	987:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	8	31	theme	ophthalmic	1354:1363	arg1	medium					1412:1417	Optisol-GS® corneal storage medium	1384:1417	Optisol-GS® corneal storage medium	1384:1417	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	31	theme	ophthalmic	1354:1363	arg1	products					1365:1372	different aqueous ophthalmic products	1336:1372	different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops	1336:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	31	theme	ophthalmic	1354:1363	arg1	solution					1434:1441	balanced salt solution	1420:1441	balanced salt solution for intraocular irrigation	1420:1468	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	31	theme	ophthalmic	1354:1363	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	14	32	theme	applied	2384:2390	arg1	complex					2407:2413	topically applied ubiquinol/γ-CD complex	2374:2413	topically applied ubiquinol/γ-CD complex	2374:2413	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	6	33	theme	dispersible	996:1006	arg1	formulation					1019:1029	a highly dispersible and stable formulation	987:1029	a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products	987:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	15	34	theme	corneal	2776:2782	arg1	endothelium					2784:2794	the corneal endothelium	2772:2794	the corneal endothelium	2772:2794	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	15	35	theme	human	2626:2630	arg1	corneas					2638:2644	human donor corneas	2626:2644	human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices	2626:2712	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	9	36	theme	high	1582:1585	arg1	metabolism					1593:1602	high basal metabolism	1582:1602	high basal metabolism	1582:1602	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	8	37	theme	free	1318:1321	arg1	ubiquinol					1323:1331	free ubiquinol	1318:1331	free ubiquinol	1318:1331	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	38	theme	corneal	1396:1402	arg1	medium					1412:1417	Optisol-GS® corneal storage medium	1384:1417	Optisol-GS® corneal storage medium	1384:1417	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	14	39	theme	same	2362:2365	arg1	model					2367:2371	the same model	2358:2371	the same model	2358:2371	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	1	40	theme	impaired	86:93	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	40	theme	impaired	86:93	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	40	theme	impaired	86:93	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	40	theme	impaired	86:93	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	3	41	theme	organs	394:399	arg1	cells					385:389	the cells	381:389	the cells of organs and tissues preserved in hypothermic conditions before surgery	381:462	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	4	42	theme	powerful	722:729	arg1	antioxidant					731:741	a powerful antioxidant	720:741	a powerful antioxidant	720:741	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	7	43	theme	strongest	1182:1190	arg1	affinity					1200:1207	the strongest binding affinity	1178:1207	the strongest binding affinity with ubiquinol compared to α- or β-CD	1178:1245	Docking studies showed that γ-CD has the strongest binding affinity with ubiquinol compared to α- or β-CD.					
34087583	15	44	theme	complex	2600:2606	arg1	penetrance					2571:2580	the penetrance	2567:2580	the penetrance of ubiquinol/γ-CD complex	2567:2606	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	9	45	theme	ROS	1608:1610	arg1	A549					1624:1627	A549	1624:1627	A549	1624:1627	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	9	45	theme	ROS	1608:1610	arg1	generation					1612:1621	ROS generation	1608:1621	ROS generation (A549)	1608:1628	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	6	46	theme	gamma	1069:1073	arg1	cyclodextrin					1075:1086	gamma cyclodextrin	1069:1086	gamma cyclodextrin (γ-CD)	1069:1093	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	6	46	theme	gamma	1069:1073	arg1	γ-CD					1089:1092	γ-CD	1089:1092	γ-CD	1089:1092	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	8	47	theme	balanced	1420:1427	arg1	solution					1434:1441	balanced salt solution	1420:1441	balanced salt solution for intraocular irrigation	1420:1468	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	16	48	theme	endothelial	3078:3088	arg1	cells					3090:3094	corneal endothelial cells	3070:3094	corneal endothelial cells	3070:3094	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	6	49	theme	ubiquinol	1055:1063	arg1	complex					1044:1050	a complex	1042:1050	a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products	1042:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	4	50	theme	donor	822:826	arg1	cells					848:852	human donor corneal endothelial cells	816:852	human donor corneal endothelial cells	816:852	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	0	51	theme	corneal	37:43	arg1	function					45:52	corneal function	37:52	corneal function	37:52	Solubilized ubiquinol for preserving corneal function.					
34087583	3	52	theme	tissues	405:411	arg1	cells					385:389	the cells	381:389	the cells of organs and tissues preserved in hypothermic conditions before surgery	381:462	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	3	53	dep	stress	364:369	arg1	case					475:478	the case	471:478	the case for cornea transplantation	471:505	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	8	54	theme	intraocular	1447:1457	arg1	irrigation					1459:1468	intraocular irrigation	1447:1468	intraocular irrigation	1447:1468	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	13	55	theme	vertical	2107:2114	arg1	system					2126:2131	a vertical diffusion system	2105:2131	a vertical diffusion system	2105:2131	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	13	56	theme	γ-CD	2295:2298	arg1	vehicle					2300:2306	the γ-CD vehicle	2291:2306	the γ-CD vehicle	2291:2306	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	14	57	theme	donor	2456:2460	arg1	corneas					2462:2468	human donor corneas	2450:2468	human donor corneas with markedly greater ubiquinol retention	2450:2510	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	4	58	theme	human	816:820	arg1	cells					848:852	human donor corneal endothelial cells	816:852	human donor corneal endothelial cells	816:852	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	11	59	theme	Complexed	1874:1882	arg1	ubiquinol					1884:1892	Complexed ubiquinol	1874:1892	Complexed ubiquinol	1874:1892	Complexed ubiquinol inhibited lipid peroxidation and protected HCEC-B4G12 cells against erastin-induced ferroptosis.					
34087583	15	60	theme	industry	2695:2702	arg1	practices					2704:2712	standard industry practices	2686:2712	standard industry practices	2686:2712	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	9	61	theme	human	1648:1652	arg1	cells					1674:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	6	62	theme	aqueous-phase	1106:1118	arg1	products					1131:1138	aqueous-phase ophthalmic products	1106:1138	aqueous-phase ophthalmic products	1106:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	8	63	theme	topical	1475:1481	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	9	64	with	treatment	1686:1694	arg1	ubiquinol/γ-CD					1701:1714	ubiquinol/γ-CD	1701:1714	ubiquinol/γ-CD compared to free ubiquinol	1701:1741	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	12	65	located	detected	2028:2035	arg2	evidence					1994:2001	No evidence	1991:2001	No evidence of cellular toxicity	1991:2022	No evidence of cellular toxicity was detected in HCEC-B4G12 cells after treatment with complexed ubiquinol.					
34087583	12	65	located	detected	2028:2035	arg1	HCEC-B4G12 cells					2040:2055	HCEC-B4G12 cells	2040:2055	HCEC-B4G12 cells	2040:2055	No evidence of cellular toxicity was detected in HCEC-B4G12 cells after treatment with complexed ubiquinol.					
34087583	9	66	theme	endothelial	1662:1672	arg1	cells					1674:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	16	67	theme	topical	3024:3030	arg1	drops					3036:3040	topical eye drops	3024:3040	topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	3024:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	6	68	from	use	1099:1101	arg1	products					1131:1138	aqueous-phase ophthalmic products	1106:1138	aqueous-phase ophthalmic products	1106:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	13	69	theme	transcorneal	2227:2238	arg1	penetrance					2240:2249	transcorneal penetrance	2227:2249	transcorneal penetrance in porcine corneas	2227:2268	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	4	70	theme	endothelial	836:846	arg1	cells					848:852	human donor corneal endothelial cells	816:852	human donor corneal endothelial cells	816:852	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	2	71	theme	oxygen	319:324	arg1	ROS					335:337	ROS	335:337	ROS	335:337	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	2	71	theme	oxygen	319:324	arg1	species					326:332	reactive oxygen species	310:332	accumulated reactive oxygen species (ROS) inside cells	298:351	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	12	72	theme	complexed	2078:2086	arg1	ubiquinol					2088:2096	complexed ubiquinol	2078:2096	complexed ubiquinol	2078:2096	No evidence of cellular toxicity was detected in HCEC-B4G12 cells after treatment with complexed ubiquinol.					
34087583	15	73	dep	demonstrated	2719:2730	arg1	retained					2760:2767	retained	2760:2767	demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol	2719:2821	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	14	74	theme	corneas	2462:2468	arg1	thickness					2437:2445	the entire thickness	2426:2445	the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium	2426:2529	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	2	75	theme	accumulated	298:308	arg1	cells					347:351	accumulated reactive oxygen species (ROS) inside cells	298:351	accumulated reactive oxygen species (ROS) inside cells	298:351	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	15	76	theme	ubiquinol	2750:2758	arg1	amounts					2739:2745	higher amounts	2732:2745	higher amounts of ubiquinol	2732:2758	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	15	76	theme	ubiquinol	2750:2758	arg1	ubiquinol					2750:2758	ubiquinol	2750:2758	ubiquinol	2750:2758	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	14	77	theme	human	2450:2454	arg1	corneas					2462:2468	human donor corneas	2450:2468	human donor corneas with markedly greater ubiquinol retention	2450:2510	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	9	78	theme	free	1728:1731	arg1	ubiquinol					1733:1741	free ubiquinol	1728:1741	free ubiquinol	1728:1741	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	14	79	from	thickness	2437:2445	arg1	endothelium					2519:2529	the endothelium	2515:2529	the endothelium	2515:2529	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	8	80	theme	Complexed	1248:1256	arg1	ubiquinol					1258:1266	Complexed ubiquinol	1248:1266	Complexed ubiquinol	1248:1266	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	16	81	theme	ophthalmic	2967:2976	arg1	use					2978:2980	ophthalmic use	2967:2980	ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	2967:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	3	82	theme	cell	547:550	arg1	death					552:556	cell death	547:556	cell death prior to surgery	547:573	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	1	83	theme	hypothermic	206:216	arg1	preservation					218:229	hypothermic preservation	206:229	hypothermic preservation prior to transplantation	206:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	8	84	theme	artificial	1492:1501	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	4	85	theme	active	688:693	arg1	form					695:698	the reduced and active form	672:698	ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant -	660:743	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	3	86	theme	decreased	579:587	arg1	survival					595:602	decreased graft survival	579:602	decreased graft survival	579:602	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	9	87	theme	Greater	1519:1525	arg1	activity					1542:1549	Greater ROS scavenging activity	1519:1549	Greater ROS scavenging activity	1519:1549	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	12	88	theme	toxicity	2015:2022	arg1	evidence					1994:2001	No evidence	1991:2001	No evidence of cellular toxicity	1991:2022	No evidence of cellular toxicity was detected in HCEC-B4G12 cells after treatment with complexed ubiquinol.					
34087583	8	89	theme	eye	1508:1510	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	7	90	with	affinity	1200:1207	arg1	ubiquinol					1214:1222	ubiquinol	1214:1222	ubiquinol	1214:1222	Docking studies showed that γ-CD has the strongest binding affinity with ubiquinol compared to α- or β-CD.					
34087583	8	91	theme	higher	1289:1294	arg1	stability					1296:1304	significantly higher stability	1275:1304	significantly higher stability	1275:1304	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	16	92	theme	corneal	2998:3004	arg1	media					3014:3018	donor corneal storage media	2992:3018	donor corneal storage media	2992:3018	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	15	93	theme	ubiquinol/γ-CD	2585:2598	arg1	complex					2600:2606	ubiquinol/γ-CD complex	2585:2606	ubiquinol/γ-CD complex	2585:2606	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	9	94	theme	scavenging	1531:1540	arg1	activity					1542:1549	Greater ROS scavenging activity	1519:1549	Greater ROS scavenging activity	1519:1549	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	14	95	theme	entire	2430:2435	arg1	thickness					2437:2445	the entire thickness	2426:2445	the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium	2426:2529	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	3	96	dep	reason	533:538	arg1	behind					540:545	behind	540:545	behind cell death prior to surgery and decreased graft survival after transplantation	540:624	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	16	97	theme	stable	2878:2883	arg1	composition					2885:2895	a highly stable composition	2869:2895	a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	2869:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	16	97	theme	stable	2878:2883	arg1	ubiquinol					2836:2844	ubiquinol	2836:2844	ubiquinol complexed with γ-CD	2836:2864	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	4	98	theme	Q10	712:714	arg1	form					695:698	the reduced and active form	672:698	ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant -	660:743	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	10	99	theme	free	1858:1861	arg1	ubiquinol					1863:1871	free ubiquinol	1858:1871	free ubiquinol	1858:1871	Furthermore, complexed ubiquinol was more effective at lowering ROS, and at far lower concentrations, compared to free ubiquinol.					
34087583	4	100	from	apoptosis	803:811	arg1	cells					848:852	human donor corneal endothelial cells	816:852	human donor corneal endothelial cells	816:852	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	1	101	theme	major	148:152	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	101	theme	major	148:152	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	101	theme	major	148:152	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	101	theme	major	148:152	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	3	102	theme	Oxidative	354:362	arg1	reason					533:538	a major reason	525:538	a major reason behind cell death prior to surgery and decreased graft survival after transplantation	525:624	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	3	102	theme	Oxidative	354:362	arg1	stress					364:369	Oxidative stress	354:369	Oxidative stress	354:369	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	4	103	theme	reduced	676:682	arg1	form					695:698	the reduced and active form	672:698	ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant -	660:743	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	14	104	theme	ubiquinol/γ-CD	2392:2405	arg1	complex					2407:2413	topically applied ubiquinol/γ-CD complex	2374:2413	topically applied ubiquinol/γ-CD complex	2374:2413	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	12	105	with	treatment	2063:2071	arg1	ubiquinol					2088:2096	complexed ubiquinol	2078:2096	complexed ubiquinol	2078:2096	No evidence of cellular toxicity was detected in HCEC-B4G12 cells after treatment with complexed ubiquinol.					
34087583	9	106	theme	cell	1566:1569	arg1	model					1571:1575	a cell model	1564:1575	a cell model with high basal metabolism and ROS generation (A549)	1564:1628	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	9	107	with	model	1571:1575	arg1	A549					1624:1627	A549	1624:1627	A549	1624:1627	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	9	107	with	model	1571:1575	arg1	metabolism					1593:1602	high basal metabolism	1582:1602	high basal metabolism	1582:1602	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	9	107	with	model	1571:1575	arg1	generation					1612:1621	ROS generation	1608:1621	ROS generation (A549)	1608:1628	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	8	108	theme	aqueous	1346:1352	arg1	medium					1412:1417	Optisol-GS® corneal storage medium	1384:1417	Optisol-GS® corneal storage medium	1384:1417	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	108	theme	aqueous	1346:1352	arg1	products					1365:1372	different aqueous ophthalmic products	1336:1372	different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops	1336:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	108	theme	aqueous	1346:1352	arg1	solution					1434:1441	balanced salt solution	1420:1441	balanced salt solution for intraocular irrigation	1420:1468	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	8	108	theme	aqueous	1346:1352	arg1	drops					1512:1516	topical Refresh® artificial tear eye drops	1475:1516	topical Refresh® artificial tear eye drops	1475:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	13	109	theme	γ-CD	2175:2178	arg1	complex					2164:2170	a topically applied inclusion complex	2134:2170	a topically applied inclusion complex of γ-CD	2134:2178	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	13	109	theme	γ-CD	2175:2178	arg1	dye					2197:2199	a lipophilic dye	2184:2199	a lipophilic dye (coumarin-6)	2184:2212	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	13	109	theme	γ-CD	2175:2178	arg1	coumarin-6					2202:2211	coumarin-6	2202:2211	coumarin-6	2202:2211	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	12	110	theme	cellular	2006:2013	arg1	toxicity					2015:2022	cellular toxicity	2006:2022	cellular toxicity	2006:2022	No evidence of cellular toxicity was detected in HCEC-B4G12 cells after treatment with complexed ubiquinol.					
34087583	1	111	theme	cellular	65:72	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	111	theme	cellular	65:72	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	111	theme	cellular	65:72	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	111	theme	cellular	65:72	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	16	112	theme	aqueous-phase	2940:2952	arg1	products					2954:2961	aqueous-phase products	2940:2961	aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage	2940:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	2	113	theme	inside	340:345	arg1	cells					347:351	accumulated reactive oxygen species (ROS) inside cells	298:351	accumulated reactive oxygen species (ROS) inside cells	298:351	These problems are thought to arise from accumulated reactive oxygen species (ROS) inside cells.					
34087583	7	114	theme	binding	1192:1198	arg1	affinity					1200:1207	the strongest binding affinity	1178:1207	the strongest binding affinity with ubiquinol compared to α- or β-CD	1178:1245	Docking studies showed that γ-CD has the strongest binding affinity with ubiquinol compared to α- or β-CD.					
34087583	15	115	theme	donor	2632:2636	arg1	corneas					2638:2644	human donor corneas	2626:2644	human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices	2626:2712	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	9	116	theme	basal	1587:1591	arg1	metabolism					1593:1602	high basal metabolism	1582:1602	high basal metabolism	1582:1602	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	4	117	theme	mitochondrial	768:780	arg1	function					782:789	mitochondrial function	768:789	mitochondrial function	768:789	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	16	118	theme	corneal	3070:3076	arg1	cells					3090:3094	corneal endothelial cells	3070:3094	corneal endothelial cells	3070:3094	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	13	119	theme	applied	2146:2152	arg1	complex					2164:2170	a topically applied inclusion complex	2134:2170	a topically applied inclusion complex of γ-CD	2134:2178	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	1	120	theme	mitochondrial	95:107	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	120	theme	mitochondrial	95:107	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	120	theme	mitochondrial	95:107	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	120	theme	mitochondrial	95:107	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	6	121	theme	cyclodextrin	1075:1086	arg1	complex					1044:1050	a complex	1042:1050	a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products	1042:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	8	122	theme	storage	1404:1410	arg1	medium					1412:1417	Optisol-GS® corneal storage medium	1384:1417	Optisol-GS® corneal storage medium	1384:1417	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	11	123	theme	erastin-induced	1962:1976	arg1	ferroptosis					1978:1988	erastin-induced ferroptosis	1962:1988	erastin-induced ferroptosis	1962:1988	Complexed ubiquinol inhibited lipid peroxidation and protected HCEC-B4G12 cells against erastin-induced ferroptosis.					
34087583	4	124	dep	ubiquinol	660:668	arg1	form					695:698	the reduced and active form	672:698	ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant -	660:743	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	5	125	theme	molecule	959:966	arg1	formulation					939:949	an aqueous-based formulation	922:949	an aqueous-based formulation of this molecule	922:966	However, ubiquinol is highly lipophilic, underscoring the need for an aqueous-based formulation of this molecule.					
34087583	8	126	dep	showed	1268:1273	arg1	compared					1306:1313	compared	1306:1313	showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops	1268:1516	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	14	127	with	corneas	2462:2468	arg1	retention					2502:2510	markedly greater ubiquinol retention	2475:2510	markedly greater ubiquinol retention	2475:2510	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	4	128	theme	antioxidant	731:741	arg1	form					695:698	the reduced and active form	672:698	ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant -	660:743	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	16	129	theme	oxidative	3104:3112	arg1	damage					3114:3119	oxidative damage	3104:3119	oxidative damage	3104:3119	In summary, ubiquinol complexed with γ-CD is a highly stable composition that can be incorporated into a variety of aqueous-phase products for ophthalmic use including donor corneal storage media and topical eye drops to scavenge ROS and protect corneal endothelial cells against oxidative damage.					
34087583	1	130	theme	prior	231:235	arg1	preservation					218:229	hypothermic preservation	206:229	hypothermic preservation prior to transplantation	206:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	3	131	theme	hypothermic	426:436	arg1	conditions					438:447	hypothermic conditions	426:447	hypothermic conditions	426:447	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	13	132	from	penetrance	2240:2249	arg1	corneas					2262:2268	porcine corneas	2254:2268	porcine corneas	2254:2268	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	8	133	theme	salt	1429:1432	arg1	solution					1434:1441	balanced salt solution	1420:1441	balanced salt solution for intraocular irrigation	1420:1468	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	14	134	theme	free	2543:2546	arg1	ubiquinol					2548:2556	free ubiquinol	2543:2556	free ubiquinol	2543:2556	Using the same model, topically applied ubiquinol/γ-CD complex penetrated the entire thickness of human donor corneas with markedly greater ubiquinol retention in the endothelium compared to free ubiquinol.					
34087583	7	135	theme	Docking	1141:1147	arg1	studies					1149:1155	Docking studies	1141:1155	Docking studies	1141:1155	Docking studies showed that γ-CD has the strongest binding affinity with ubiquinol compared to α- or β-CD.					
34087583	13	136	from	capacity	2278:2285	arg1	corneas					2262:2268	porcine corneas	2254:2268	porcine corneas	2254:2268	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	4	137	theme	corneal	828:834	arg1	cells					848:852	human donor corneal endothelial cells	816:852	human donor corneal endothelial cells	816:852	We have recently discovered that ubiquinol - the reduced and active form of coenzyme Q10 and a powerful antioxidant - significantly enhances mitochondrial function and reduces apoptosis in human donor corneal endothelial cells.					
34087583	13	138	theme	diffusion	2116:2124	arg1	system					2126:2131	a vertical diffusion system	2105:2131	a vertical diffusion system	2105:2131	Using a vertical diffusion system, a topically applied inclusion complex of γ-CD and a lipophilic dye (coumarin-6) demonstrated transcorneal penetrance in porcine corneas and the capacity for the γ-CD vehicle to deliver drug to the corneal endothelium.					
34087583	11	139	theme	lipid	1904:1908	arg1	peroxidation					1910:1921	lipid peroxidation	1904:1921	lipid peroxidation	1904:1921	Complexed ubiquinol inhibited lipid peroxidation and protected HCEC-B4G12 cells against erastin-induced ferroptosis.					
34087583	15	140	theme	standard	2686:2693	arg1	practices					2704:2712	standard industry practices	2686:2712	standard industry practices	2686:2712	Lastly, the penetrance of ubiquinol/γ-CD complex was assayed using human donor corneas preserved for 7 days in Optisol-GS® per standard industry practices, and demonstrated higher amounts of ubiquinol retained in the corneal endothelium compared to free ubiquinol.					
34087583	1	141	theme	Defective	55:63	arg1	death					138:142	increased cell death	123:142	increased cell death	123:142	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	141	theme	Defective	55:63	arg1	problems					154:161	major problems	148:161	major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation	148:254	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	141	theme	Defective	55:63	arg1	metabolism					74:83	Defective cellular metabolism	55:83	Defective cellular metabolism	55:83	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	1	141	theme	Defective	55:63	arg1	function					109:116	impaired mitochondrial function	86:116	impaired mitochondrial function	86:116	Defective cellular metabolism, impaired mitochondrial function, and increased cell death are major problems that adversely affect donor tissues during hypothermic preservation prior to transplantation.					
34087583	9	142	theme	HCEC-B4G12	1637:1646	arg1	cells					1674:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34087583	6	143	theme	ophthalmic	1120:1129	arg1	products					1131:1138	aqueous-phase ophthalmic products	1106:1138	aqueous-phase ophthalmic products	1106:1138	Herein, we report a highly dispersible and stable formulation comprising a complex of ubiquinol and gamma cyclodextrin (γ-CD) for use in aqueous-phase ophthalmic products.					
34087583	8	144	theme	Optisol-GS®	1384:1394	arg1	medium					1412:1417	Optisol-GS® corneal storage medium	1384:1417	Optisol-GS® corneal storage medium	1384:1417	Complexed ubiquinol showed significantly higher stability compared to free ubiquinol in different aqueous ophthalmic products including Optisol-GS® corneal storage medium, balanced salt solution for intraocular irrigation, and topical Refresh® artificial tear eye drops.					
34087583	3	145	theme	cornea	484:489	arg1	transplantation					491:505	cornea transplantation	484:505	cornea transplantation	484:505	Oxidative stress acting on the cells of organs and tissues preserved in hypothermic conditions before surgery, as is the case for cornea transplantation, is thought to be a major reason behind cell death prior to surgery and decreased graft survival after transplantation.					
34087583	9	146	theme	corneal	1654:1660	arg1	cells					1674:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	HCEC-B4G12 human corneal endothelial cells	1637:1678	Greater ROS scavenging activity was noted in a cell model with high basal metabolism and ROS generation (A549) and in HCEC-B4G12 human corneal endothelial cells after treatment with ubiquinol/γ-CD compared to free ubiquinol.					
34980380	0	0	theme	acute	70:74	arg1	colitis					76:82	acute colitis	70:82	acute colitis	70:82	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	6	1	theme	fatty	909:913	arg1	acids					915:919	fecal short-chain fatty acids	891:919	fecal short-chain fatty acids	891:919	In addition, MYP supplementation enriched the contents of fecal short-chain fatty acids.					
34980380	1	2	theme	Inflammatory	150:161	arg1	disease					169:175	Inflammatory bowel disease	150:175	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	6	3	theme	short-chain	897:907	arg1	acids					915:919	fecal short-chain fatty acids	891:919	fecal short-chain fatty acids	891:919	In addition, MYP supplementation enriched the contents of fecal short-chain fatty acids.					
34980380	1	4	theme	bowel	163:167	arg1	disease					169:175	Inflammatory bowel disease	150:175	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	2	5	theme	study	264:268	arg1	objection					246:254	The objection	242:254	The objection of this study	242:268	The objection of this study is to investigate whether the major yolk protein (MYP) could aid in the remission of colitis.					
34980380	7	6	theme	acids	1086:1090	arg1	level					1092:1096	short-chain fatty acids level	1068:1096	short-chain fatty acids level	1068:1096	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	6	7	theme	fecal	891:895	arg1	acids					915:919	fecal short-chain fatty acids	891:919	fecal short-chain fatty acids	891:919	In addition, MYP supplementation enriched the contents of fecal short-chain fatty acids.					
34980380	7	8	theme	MYP	941:943	arg1	alleviation					926:936	The alleviation	922:936	The alleviation of MYP on the colitis	922:958	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	4	9	theme	MYP	568:570	arg1	group					572:576	the MYP group	564:576	the MYP group	564:576	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	7	10	theme	short-chain	1068:1078	arg1	acids					1086:1090	short-chain fatty acids	1068:1090	short-chain fatty acids level	1068:1096	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	1	11	theme	disease	169:175	arg1	individuals					187:197	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	5	12	theme	MYP	738:740	arg1	supplementation					719:733	supplementation	719:733	supplementation of MYP	719:740	The results also showed that supplementation of MYP improved the shift in microbial community composition of mice with colitis induced by DSS.					
34980380	0	13	from	cucumber	28:35	arg1	protein					11:17	Major yolk protein	0:17	Major yolk protein from sea cucumber (Stichopus japonicus)	0:57	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	7	14	theme	fatty	1080:1084	arg1	acids					1086:1090	short-chain fatty acids	1068:1090	short-chain fatty acids level	1068:1096	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	1	15	theme	afflicted	177:185	arg1	individuals					187:197	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	0	16	theme	yolk	6:9	arg1	protein					11:17	Major yolk protein	0:17	Major yolk protein from sea cucumber (Stichopus japonicus)	0:57	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	3	17	from	function	368:375	arg1	colitis					393:399	acute colitis	387:399	acute colitis	387:399	The function of MYP on acute colitis was assessed through a dextran sulfate sodium -induced colitis mice model.					
34980380	4	18	theme	model	663:667	arg1	group					683:687	the model and treatment group	659:687	group	683:687	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	0	19	theme	Major	0:4	arg1	protein					11:17	Major yolk protein	0:17	Major yolk protein from sea cucumber (Stichopus japonicus)	0:57	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	7	20	from	increase	1056:1063	arg1	level					1092:1096	short-chain fatty acids level	1068:1096	short-chain fatty acids level	1068:1096	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	2	21	theme	colitis	355:361	arg1	remission					342:350	the remission	338:350	the remission of colitis	338:361	The objection of this study is to investigate whether the major yolk protein (MYP) could aid in the remission of colitis.					
34980380	8	22	theme	oral	1164:1167	arg1	candidate					1192:1200	a potential candidate	1180:1200	a potential candidate for the attenuating of acute colitis	1180:1237	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	8	22	theme	oral	1164:1167	arg1	MYP					1169:1171	oral MYP	1164:1171	oral MYP	1164:1171	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	4	23	theme	anti-inflammatory	509:525	arg1	cytokines					527:535	the anti-inflammatory cytokines	505:535	the anti-inflammatory cytokines	505:535	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	0	24	theme	dysbiosis	112:120	arg1	regulation					88:97	regulation	88:97	regulation of microbial dysbiosis	88:120	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	0	24	theme	dysbiosis	112:120	arg1	responses					139:147	inflammatory responses	126:147	inflammatory responses	126:147	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	2	25	theme	yolk	306:309	arg1	MYP					320:322	MYP	320:322	MYP	320:322	The objection of this study is to investigate whether the major yolk protein (MYP) could aid in the remission of colitis.					
34980380	2	25	theme	yolk	306:309	arg1	protein					311:317	major yolk protein	300:317	the major yolk protein (MYP)	296:323	The objection of this study is to investigate whether the major yolk protein (MYP) could aid in the remission of colitis.					
34980380	1	26	theme	most	203:206	arg1	medications					208:218	most medications	203:218	most medications	203:218	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	0	27	theme	microbial	102:110	arg1	dysbiosis					112:120	microbial dysbiosis	102:120	microbial dysbiosis	102:120	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	2	28	theme	major	300:304	arg1	MYP					320:322	MYP	320:322	MYP	320:322	The objection of this study is to investigate whether the major yolk protein (MYP) could aid in the remission of colitis.					
34980380	2	28	theme	major	300:304	arg1	protein					311:317	major yolk protein	300:317	the major yolk protein (MYP)	296:323	The objection of this study is to investigate whether the major yolk protein (MYP) could aid in the remission of colitis.					
34980380	5	29	theme	microbial	764:772	arg1	composition					784:794	microbial community composition	764:794	microbial community composition of mice with colitis induced by DSS	764:830	The results also showed that supplementation of MYP improved the shift in microbial community composition of mice with colitis induced by DSS.					
34980380	0	30	theme	inflammatory	126:137	arg1	responses					139:147	inflammatory responses	126:147	inflammatory responses	126:147	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	6	31	theme	MYP	846:848	arg1	supplementation					850:864	MYP supplementation	846:864	MYP supplementation	846:864	In addition, MYP supplementation enriched the contents of fecal short-chain fatty acids.					
34980380	8	32	theme	colitis	1231:1237	arg1	attenuating					1210:1220	the attenuating	1206:1220	the attenuating of acute colitis	1206:1237	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	0	33	theme	sea	24:26	arg1	cucumber					28:35	sea cucumber	24:35	sea cucumber	24:35	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	3	34	theme	acute	387:391	arg1	colitis					393:399	acute colitis	387:399	acute colitis	387:399	The function of MYP on acute colitis was assessed through a dextran sulfate sodium -induced colitis mice model.					
34980380	7	35	theme	dysbiosis	995:1003	arg1	state					1005:1009	the dysbiosis state	991:1009	the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level	991:1096	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	1	36	theme	adverse	225:231	arg1	effects					233:239	adverse effects	225:239	adverse effects	225:239	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	7	37	theme	anti-inflammatory	1110:1126	arg1	IL-10					1148:1152	IL-10	1148:1152	IL-10	1148:1152	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	7	37	theme	anti-inflammatory	1110:1126	arg1	IL-4					1139:1142	IL-4	1139:1142	IL-4	1139:1142	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	7	37	theme	anti-inflammatory	1110:1126	arg1	cytokines					1128:1136	anti-inflammatory cytokines	1110:1136	anti-inflammatory cytokines (IL-4 and IL-10)	1110:1153	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	4	38	theme	model	492:496	arg1	group					498:502	the model group	488:502	the model group	488:502	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	7	39	theme	microbiota	1022:1031	arg1	state					1005:1009	the dysbiosis state	991:1009	the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level	991:1096	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	3	40	theme	mice	464:467	arg1	model					469:473	a dextran sulfate sodium -induced colitis mice model	422:473	a dextran sulfate sodium -induced colitis mice model	422:473	The function of MYP on acute colitis was assessed through a dextran sulfate sodium -induced colitis mice model.					
34980380	3	41	theme	MYP	380:382	arg1	function					368:375	The function	364:375	The function of MYP on acute colitis	364:399	The function of MYP on acute colitis was assessed through a dextran sulfate sodium -induced colitis mice model.					
34980380	5	42	from	shift	755:759	arg1	composition					784:794	microbial community composition	764:794	microbial community composition of mice with colitis induced by DSS	764:830	The results also showed that supplementation of MYP improved the shift in microbial community composition of mice with colitis induced by DSS.					
34980380	7	43	dep	cytokines	1128:1136	arg1	IL-10					1148:1152	IL-10	1148:1152	IL-10	1148:1152	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	7	43	dep	cytokines	1128:1136	arg1	IL-4					1139:1142	IL-4	1139:1142	IL-4	1139:1142	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	7	43	dep	cytokines	1128:1136	arg1	cytokines					1128:1136	anti-inflammatory cytokines	1110:1136	anti-inflammatory cytokines (IL-4 and IL-10)	1110:1153	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	8	44	from	candidate	1192:1200	arg1	sum					1159:1161	sum	1159:1161	sum	1159:1161	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	1	45	contain	have	220:223	arg1	individuals					187:197	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals	150:197	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	1	45	contain	have	220:223	arg2	effects					233:239	adverse effects	225:239	adverse effects	225:239	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	1	45	contain	have	220:223	arg1	medications					208:218	most medications	203:218	most medications	203:218	Inflammatory bowel disease afflicted individuals and most medications have adverse effects.					
34980380	3	46	theme	-induced	447:454	arg1	model					469:473	a dextran sulfate sodium -induced colitis mice model	422:473	a dextran sulfate sodium -induced colitis mice model	422:473	The function of MYP on acute colitis was assessed through a dextran sulfate sodium -induced colitis mice model.					
34980380	5	47	with	mice	799:802	arg1	colitis					809:815	colitis	809:815	colitis induced by DSS	809:830	The results also showed that supplementation of MYP improved the shift in microbial community composition of mice with colitis induced by DSS.					
34980380	7	48	theme	colonic	1014:1020	arg1	microbiota					1022:1031	colonic microbiota	1014:1031	colonic microbiota	1014:1031	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	0	49	dep	protein	11:17	arg1	japonicus					48:56	Stichopus japonicus	38:56	Stichopus japonicus	38:56	Major yolk protein from sea cucumber (Stichopus japonicus) attenuates acute colitis via regulation of microbial dysbiosis and inflammatory responses.					
34980380	3	50	theme	colitis	456:462	arg1	model					469:473	a dextran sulfate sodium -induced colitis mice model	422:473	a dextran sulfate sodium -induced colitis mice model	422:473	The function of MYP on acute colitis was assessed through a dextran sulfate sodium -induced colitis mice model.					
34980380	7	51	from	alleviation	926:936	arg1	colitis					952:958	the colitis	948:958	the colitis	948:958	The alleviation of MYP on the colitis was probably related to repair the dysbiosis state of colonic microbiota, which thus induced an increase in short-chain fatty acids level and secrete anti-inflammatory cytokines (IL-4 and IL-10).					
34980380	8	52	theme	acute	1225:1229	arg1	colitis					1231:1237	acute colitis	1225:1237	acute colitis	1225:1237	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	4	53	theme	treatment	673:681	arg1	group					683:687	the model and treatment group	659:687	group	683:687	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	8	54	theme	potential	1182:1190	arg1	candidate					1192:1200	a potential candidate	1180:1200	a potential candidate for the attenuating of acute colitis	1180:1237	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	8	54	theme	potential	1182:1190	arg1	MYP					1169:1171	oral MYP	1164:1171	oral MYP	1164:1171	In sum, oral MYP may be a potential candidate for the attenuating of acute colitis.					
34980380	4	55	theme	pro-inflammatory	591:606	arg1	cytokines					608:616	the pro-inflammatory cytokines	587:616	the pro-inflammatory cytokines	587:616	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	4	55	theme	pro-inflammatory	591:606	arg1	different					641:649	different	641:649	different	641:649	Compared to the model group, the anti-inflammatory cytokines increased significantly in the MYP group, whereas the pro-inflammatory cytokines were not significantly different between the model and treatment group.					
34980380	6	56	theme	acids	915:919	arg1	contents					879:886	the contents	875:886	the contents of fecal short-chain fatty acids	875:919	In addition, MYP supplementation enriched the contents of fecal short-chain fatty acids.					
34980380	5	57	theme	community	774:782	arg1	composition					784:794	microbial community composition	764:794	microbial community composition of mice with colitis induced by DSS	764:830	The results also showed that supplementation of MYP improved the shift in microbial community composition of mice with colitis induced by DSS.					
34980380	5	58	theme	mice	799:802	arg1	composition					784:794	microbial community composition	764:794	microbial community composition of mice with colitis induced by DSS	764:830	The results also showed that supplementation of MYP improved the shift in microbial community composition of mice with colitis induced by DSS.					
33252172	5	0	theme	PVA/pectin	711:720	arg1	films					722:726	PVA/pectin films	711:726	PVA/pectin films	711:726	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	6	1	theme	PVA/pectin	877:886	arg1	films					888:892	PVA/pectin films	877:892	PVA/pectin films	877:892	After both ultraviolet and plasma treatment, the hydrophilicity of PVA/pectin films increased.					
33252172	9	2	theme	fibroblast-like	1323:1337	arg1	cells					1339:1343	fibroblast-like cells	1323:1343	fibroblast-like cells	1323:1343	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	1	3	theme	dressings	140:148	arg1	design					118:123	The design	114:123	The design of novel wound dressings for chronic wound treatment	114:176	The design of novel wound dressings for chronic wound treatment is still of great importance.					
33252172	11	4	with	films	1496:1500	arg1	MSCs					1515:1518	adhered MSCs	1507:1518	adhered MSCs	1507:1518	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	5	5	theme	films	722:726	arg1	surface					700:706	the surface	696:706	the surface of PVA/pectin films	696:726	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	2	6	theme	polymer	315:321	arg1	film					323:326	a flexible polymer film	304:326	a flexible polymer film	304:326	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	0	7	theme	stem	91:94	arg1	cells					96:100	mesenchymal stem cells	79:100	mesenchymal stem cells	79:100	Polyvinyl alcohol and pectin blended films: Preparation, characterization, and mesenchymal stem cells attachment.					
33252172	10	8	theme	MSCs	1435:1438	arg1	adhesion					1440:1447	MSCs adhesion	1435:1447	MSCs adhesion	1435:1447	The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion.					
33252172	8	9	theme	cell	1164:1167	arg1	behavior					1169:1176	cell behavior	1164:1176	cell behavior	1164:1176	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33252172	7	10	theme	additional	928:937	arg1	crosslinking					939:950	additional crosslinking	928:950	additional crosslinking of PVA/pectin films with glutaraldehyde	928:990	It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure.					
33252172	3	11	theme	casting	477:483	arg1	method					485:490	solution casting method	468:490	solution casting method	468:490	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	4	12	theme	formed	541:546	arg1	films					559:563	the formed PVA/pectin films	537:563	the formed PVA/pectin films	537:563	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	0	13	theme	mesenchymal	79:89	arg1	cells					96:100	mesenchymal stem cells	79:100	mesenchymal stem cells	79:100	Polyvinyl alcohol and pectin blended films: Preparation, characterization, and mesenchymal stem cells attachment.					
33252172	9	14	theme	monolayer	1302:1310	arg1	culture					1312:1318	the monolayer culture	1298:1318	them the monolayer culture of fibroblast-like cells	1293:1343	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	11	15	theme	potential	1541:1549	arg1	application					1551:1561	potential application	1541:1561	potential application	1541:1561	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	2	16	theme	stem	270:273	arg1	MSCs					282:285	MSCs	282:285	MSCs	282:285	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	2	16	theme	stem	270:273	arg1	cells					275:279	mesenchymal stem cells	258:279	mesenchymal stem cells (MSCs)	258:286	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	11	17	theme	novel	1576:1580	arg1	dressings					1596:1604	novel complex wound dressings	1576:1604	novel complex wound dressings	1576:1604	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	3	18	theme	different	420:428	arg1	ratio					440:444	different component ratio	420:444	different component ratio	420:444	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	3	19	with	pectin	408:413	arg1	ratio					440:444	different component ratio	420:444	different component ratio	420:444	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	5	20	theme	plasma	793:798	arg1	exposure					800:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	2	21	theme	mesenchymal	258:268	arg1	MSCs					282:285	MSCs	282:285	MSCs	282:285	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	2	21	theme	mesenchymal	258:268	arg1	cells					275:279	mesenchymal stem cells	258:279	mesenchymal stem cells (MSCs)	258:286	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	1	22	theme	chronic	154:160	arg1	treatment					168:176	chronic wound treatment	154:176	chronic wound treatment	154:176	The design of novel wound dressings for chronic wound treatment is still of great importance.					
33252172	8	23	theme	composition	1149:1159	arg1	effects					1113:1119	the effects	1109:1119	the effects of film characteristics and composition on cell behavior	1109:1176	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33252172	10	24	theme	adhesion	1440:1447	arg1	enhancement					1420:1430	enhancement	1420:1430	enhancement of MSCs adhesion	1420:1447	The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion.					
33252172	3	25	theme	solution	468:475	arg1	method					485:490	solution casting method	468:490	solution casting method	468:490	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	10	26	with	modification	1361:1372	arg1	collagen					1399:1406	collagen	1399:1406	collagen	1399:1406	The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion.					
33252172	1	27	theme	wound	162:166	arg1	treatment					168:176	chronic wound treatment	154:176	chronic wound treatment	154:176	The design of novel wound dressings for chronic wound treatment is still of great importance.					
33252172	5	28	theme	ultraviolet	749:759	arg1	exposure					800:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	6	29	theme	plasma	837:842	arg1	treatment					844:852	plasma treatment	837:852	plasma treatment	837:852	After both ultraviolet and plasma treatment, the hydrophilicity of PVA/pectin films increased.					
33252172	9	30	dep	them	1293:1296	arg1	culture					1312:1318	the monolayer culture	1298:1318	them the monolayer culture of fibroblast-like cells	1293:1343	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	8	31	theme	PVA/pectin	1078:1087	arg1	samples					1089:1095	modified PVA/pectin samples	1069:1095	modified PVA/pectin samples	1069:1095	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33252172	0	32	theme	Polyvinyl	0:8	arg1	alcohol					10:16	Polyvinyl alcohol	0:16	Polyvinyl alcohol	0:16	Polyvinyl alcohol and pectin blended films: Preparation, characterization, and mesenchymal stem cells attachment.					
33252172	9	33	theme	cells	1339:1343	arg1	culture					1312:1318	the monolayer culture	1298:1318	them the monolayer culture of fibroblast-like cells	1293:1343	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	11	34	theme	complex	1582:1588	arg1	dressings					1596:1604	novel complex wound dressings	1576:1604	novel complex wound dressings	1576:1604	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	8	35	theme	film	1124:1127	arg1	characteristics					1129:1143	film characteristics	1124:1143	film characteristics	1124:1143	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33252172	5	36	theme	dielectric	764:773	arg1	exposure					800:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	11	37	theme	PVA/pectin	1485:1494	arg1	films					1496:1500	the obtained PVA/pectin films	1472:1500	the obtained PVA/pectin films with adhered MSCs	1472:1518	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	11	37	theme	PVA/pectin	1485:1494	arg1	part					1568:1571	a part	1566:1571	a part of novel complex wound dressings	1566:1604	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	5	38	theme	discharge	783:791	arg1	exposure					800:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	5	39	theme	barrier	775:781	arg1	exposure					800:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	4	40	theme	Physicochemical	507:521	arg1	properties					523:532	Physicochemical properties	507:532	Physicochemical properties	507:532	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	4	40	theme	Physicochemical	507:521	arg1	morphology					582:591	their morphology	576:591	their morphology	576:591	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	4	41	theme	mechanical	628:637	arg1	characteristics					639:653	mechanical characteristics	628:653	mechanical characteristics	628:653	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	4	41	theme	mechanical	628:637	arg1	morphology					582:591	their morphology	576:591	their morphology	576:591	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	3	42	theme	blended	357:363	arg1	films					365:369	blended films	357:369	blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio	357:444	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	8	43	theme	modified	1069:1076	arg1	samples					1089:1095	modified PVA/pectin samples	1069:1095	modified PVA/pectin samples	1069:1095	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33252172	7	44	with	crosslinking	939:950	arg1	glutaraldehyde					977:990	glutaraldehyde	977:990	glutaraldehyde	977:990	It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure.					
33252172	1	45	theme	great	190:194	arg1	importance					196:205	great importance	190:205	great importance	190:205	The design of novel wound dressings for chronic wound treatment is still of great importance.					
33252172	3	46	theme	polyvinyl	380:388	arg1	PVA					399:401	PVA	399:401	PVA	399:401	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	3	46	theme	polyvinyl	380:388	arg1	alcohol					390:396	polyvinyl alcohol	380:396	polyvinyl alcohol (PVA)	380:402	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	5	47	mod	modified	737:744	arg3	exposure					800:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	ultraviolet or dielectric barrier discharge plasma exposure	749:807	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	5	47	mod	modified	737:744	arg1	surface					700:706	the surface	696:706	the surface of PVA/pectin films	696:726	We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure.					
33252172	8	48	from	effects	1113:1119	arg1	behavior					1169:1176	cell behavior	1164:1176	cell behavior	1164:1176	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33252172	10	49	theme	additional	1350:1359	arg1	modification					1361:1372	The additional modification	1346:1372	The additional modification of PVA/pectin films with collagen	1346:1406	The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion.					
33252172	0	50	dep	films	37:41	arg1	attachment					102:111	attachment	102:111	attachment	102:111	Polyvinyl alcohol and pectin blended films: Preparation, characterization, and mesenchymal stem cells attachment.					
33252172	10	51	theme	films	1388:1392	arg1	modification					1361:1372	The additional modification	1346:1372	The additional modification of PVA/pectin films with collagen	1346:1406	The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion.					
33252172	2	52	theme	flexible	306:313	arg1	film					323:326	a flexible polymer film	304:326	a flexible polymer film	304:326	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	7	53	theme	structure	1027:1035	arg1	reinforcement					1004:1016	reinforcement	1004:1016	reinforcement of their structure	1004:1035	It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure.					
33252172	9	54	theme	glutaraldehyde-crosslinked	1235:1260	arg1	films					1273:1277	glutaraldehyde-crosslinked PVA/pectin films	1235:1277	glutaraldehyde-crosslinked PVA/pectin films	1235:1277	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	11	55	theme	wound	1590:1594	arg1	dressings					1596:1604	novel complex wound dressings	1576:1604	novel complex wound dressings	1576:1604	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	3	56	theme	component	430:438	arg1	ratio					440:444	different component ratio	420:444	different component ratio	420:444	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	11	57	theme	adhered	1507:1513	arg1	MSCs					1515:1518	adhered MSCs	1507:1518	adhered MSCs	1507:1518	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	9	58	theme	PVA/pectin	1262:1271	arg1	films					1273:1277	glutaraldehyde-crosslinked PVA/pectin films	1235:1277	glutaraldehyde-crosslinked PVA/pectin films	1235:1277	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	11	59	theme	dressings	1596:1604	arg1	films					1496:1500	the obtained PVA/pectin films	1472:1500	the obtained PVA/pectin films with adhered MSCs	1472:1518	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	11	59	theme	dressings	1596:1604	arg1	part					1568:1571	a part	1566:1571	a part of novel complex wound dressings	1566:1604	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	4	60	theme	films	559:563	arg1	properties					523:532	Physicochemical properties	507:532	Physicochemical properties	507:532	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	4	60	theme	films	559:563	arg1	morphology					582:591	their morphology	576:591	their morphology	576:591	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	3	61	with	alcohol	390:396	arg1	ratio					440:444	different component ratio	420:444	different component ratio	420:444	In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.					
33252172	2	62	theme	cells	275:279	arg1	application					243:253	application	243:253	application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing	243:339	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	7	63	theme	PVA/pectin	955:964	arg1	films					966:970	PVA/pectin films	955:970	PVA/pectin films	955:970	It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure.					
33252172	9	64	link	glutaraldehyde-crosslinked	1235:1260	arg1	films					1273:1277	glutaraldehyde-crosslinked PVA/pectin films	1235:1277	glutaraldehyde-crosslinked PVA/pectin films	1235:1277	It has been determined that MSCs effectively adhered to glutaraldehyde-crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast-like cells.					
33252172	4	65	theme	PVA/pectin	548:557	arg1	films					559:563	the formed PVA/pectin films	537:563	the formed PVA/pectin films	537:563	Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied.					
33252172	10	66	theme	PVA/pectin	1377:1386	arg1	films					1388:1392	PVA/pectin films	1377:1392	PVA/pectin films	1377:1392	The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion.					
33252172	2	67	theme	approaches	229:238	arg1	approaches					229:238	the promising approaches	215:238	the promising approaches	215:238	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	2	67	theme	approaches	229:238	arg1	One					208:210	One	208:210	One	208:210	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	7	68	theme	films	966:970	arg1	crosslinking					939:950	additional crosslinking	928:950	additional crosslinking of PVA/pectin films with glutaraldehyde	928:990	It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure.					
33252172	1	69	theme	novel	128:132	arg1	dressings					140:148	novel wound dressings	128:148	novel wound dressings	128:148	The design of novel wound dressings for chronic wound treatment is still of great importance.					
33252172	11	70	theme	obtained	1476:1483	arg1	films					1496:1500	the obtained PVA/pectin films	1472:1500	the obtained PVA/pectin films with adhered MSCs	1472:1518	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	11	70	theme	obtained	1476:1483	arg1	part					1568:1571	a part	1566:1571	a part of novel complex wound dressings	1566:1604	Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.					
33252172	6	71	theme	films	888:892	arg1	hydrophilicity					859:872	the hydrophilicity	855:872	the hydrophilicity of PVA/pectin films	855:892	After both ultraviolet and plasma treatment, the hydrophilicity of PVA/pectin films increased.					
33252172	2	72	theme	promising	219:227	arg1	approaches					229:238	the promising approaches	215:238	the promising approaches	215:238	One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing.					
33252172	1	73	theme	wound	134:138	arg1	dressings					140:148	novel wound dressings	128:148	novel wound dressings	128:148	The design of novel wound dressings for chronic wound treatment is still of great importance.					
33252172	8	74	theme	characteristics	1129:1143	arg1	effects					1113:1119	the effects	1109:1119	the effects of film characteristics and composition on cell behavior	1109:1176	MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior.					
33405347	0	0	theme	Cell	115:118	arg1	Application					129:139	Cell Delivery Application	115:139	Cell Delivery Application	115:139	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	2	1	dep	%	474:474	arg1	w/v					476:478	w/v	476:478	0.1% w/v	471:478	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	5	2	theme	cultivation	1065:1075	arg1	21 d					1057:1060	21 d	1057:1060	21 d of cultivation	1057:1075	The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation.					
33405347	1	3	theme	bioactive	182:190	arg1	microparticles					203:216	45S5 bioactive glass (BG) microparticles	177:216	45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	177:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	3	theme	bioactive	182:190	arg1	size					233:236	mean particle size ≈ 2 µm	219:243	mean particle size ≈ 2 µm	219:243	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	4	4	theme	degradation	854:864	arg1	rate					866:869	the degradation rate	850:869	the degradation rate	850:869	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	5	5	theme	cultivation	1009:1019	arg1	7 d					1002:1004	the first 7 d	992:1004	the first 7 d of cultivation	992:1019	The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation.					
33405347	1	6	theme	glass	192:196	arg1	microparticles					203:216	45S5 bioactive glass (BG) microparticles	177:216	45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	177:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	6	theme	glass	192:196	arg1	size					233:236	mean particle size ≈ 2 µm	219:243	mean particle size ≈ 2 µm	219:243	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	8	7	theme	promising	1334:1342	arg1	potential					1344:1352	promising potential	1334:1352	promising potential for cell delivery in bone regeneration applications	1334:1404	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	4	8	theme	materials	907:915	arg1	bioactivity					888:898	the bioactivity	884:898	the bioactivity of the materials	884:915	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	7	9	theme	cells	1251:1255	arg1	activity					1239:1246	the osteogenic activity	1224:1246	the osteogenic activity of cells incorporated in hydrogel capsules	1224:1289	The results show that 45S5 BG can positively regulate the osteogenic activity of cells incorporated in hydrogel capsules.					
33405347	3	10	theme	average	546:552	arg1	diameter					554:561	average diameter	546:561	average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively	546:792	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	1	11	theme	physicochemical	269:283	arg1	properties					285:294	physicochemical properties	269:294	physicochemical properties	269:294	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	0	12	theme	Delivery	120:127	arg1	Application					129:139	Cell Delivery Application	115:139	Cell Delivery Application	115:139	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	6	13	contain	containing	1114:1123	arg1	samples					1106:1112	samples	1106:1112	samples containing BG	1106:1126	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	6	13	contain	containing	1114:1123	arg2	BG					1125:1126	BG	1125:1126	BG	1125:1126	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	3	14	theme	%	761:761	arg1	w/v					763:765	0.1% w/v	758:765	0.1% w/v	758:765	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	6	15	theme	cell	1088:1091	arg1	viability					1093:1101	The early cell viability	1078:1101	The early cell viability in samples containing BG	1078:1126	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	6	15	theme	cell	1088:1091	arg1	lower					1131:1135	lower	1131:1135	lower	1131:1135	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	2	16	contain	containing	460:469	arg1	samples					452:458	the samples	448:458	the samples containing 0.1% w/v and 0.5% w/v BG, respectively	448:508	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	2	16	contain	containing	460:469	arg2	%					474:474	0.1%	471:474	0.1% w/v	471:478	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	2	16	contain	containing	460:469	arg2	%					487:487	0.5%	484:487	0.5% w/v BG	484:494	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	5	17	theme	MG-63	935:939	arg1	cells					941:945	MG-63 cells	935:945	MG-63 cells encapsulated in all samples	935:973	The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation.					
33405347	7	18	theme	hydrogel	1273:1280	arg1	capsules					1282:1289	hydrogel capsules	1273:1289	hydrogel capsules	1273:1289	The results show that 45S5 BG can positively regulate the osteogenic activity of cells incorporated in hydrogel capsules.					
33405347	5	19	theme	cells	941:945	arg1	viability					922:930	The viability	918:930	The viability of MG-63 cells encapsulated in all samples	918:973	The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation.					
33405347	1	20	theme	BG	199:200	arg1	microparticles					203:216	45S5 bioactive glass (BG) microparticles	177:216	45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	177:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	20	theme	BG	199:200	arg1	size					233:236	mean particle size ≈ 2 µm	219:243	mean particle size ≈ 2 µm	219:243	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	4	21	from	presence	799:806	arg1	capsules					831:838	the capsules	827:838	the capsules	827:838	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	4	22	theme	particles	814:822	arg1	presence					799:806	The presence	795:806	The presence of BG particles in the capsules	795:838	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	4	23	theme	BG	811:812	arg1	particles					814:822	BG particles	811:822	BG particles	811:822	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	1	24	from	effect	146:151	arg1	fabrication					253:263	fabrication	253:263	fabrication	253:263	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	24	from	effect	146:151	arg1	properties					285:294	physicochemical properties	269:294	physicochemical properties	269:294	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	3	25	contain	containing	723:732	arg2	BG					734:735	BG	734:735	BG	734:735	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	3	25	contain	containing	723:732	arg1	samples					715:721	the samples	711:721	the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively	711:792	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	7	26	theme	osteogenic	1228:1237	arg1	activity					1239:1246	the osteogenic activity	1224:1246	the osteogenic activity of cells incorporated in hydrogel capsules	1224:1289	The results show that 45S5 BG can positively regulate the osteogenic activity of cells incorporated in hydrogel capsules.					
33405347	1	27	theme	microparticles	203:216	arg1	incorporation					160:172	the incorporation	156:172	the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	156:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	28	theme	alginate	299:306	arg1	capsules					336:343	alginate dialdehyde-gelatin hydrogel capsules	299:343	alginate dialdehyde-gelatin hydrogel capsules	299:343	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	5	29	theme	first	996:1000	arg1	7 d					1002:1004	the first 7 d	992:1004	the first 7 d of cultivation	992:1019	The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation.					
33405347	6	30	from	viability	1093:1101	arg1	samples					1106:1112	samples	1106:1112	samples containing BG	1106:1126	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	1	31	theme	dialdehyde-gelatin	308:325	arg1	capsules					336:343	alginate dialdehyde-gelatin hydrogel capsules	299:343	alginate dialdehyde-gelatin hydrogel capsules	299:343	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	8	32	theme	composite	1307:1315	arg1	capsules					1317:1324	The fabricated composite capsules	1292:1324	The fabricated composite capsules	1292:1324	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	2	33	theme	particles	381:389	arg1	addition					366:373	The addition	362:373	The addition of BG particles	362:389	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	1	34	theme	mean	219:222	arg1	microparticles					203:216	45S5 bioactive glass (BG) microparticles	177:216	45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	177:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	34	theme	mean	219:222	arg1	size					233:236	mean particle size ≈ 2 µm	219:243	mean particle size ≈ 2 µm	219:243	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	0	35	theme	Dialdehyde-Gelatin	48:65	arg1	Microcapsules					67:79	Alginate Dialdehyde-Gelatin Microcapsules	39:79	Alginate Dialdehyde-Gelatin Microcapsules	39:79	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	1	36	theme	hydrogel	327:334	arg1	capsules					336:343	alginate dialdehyde-gelatin hydrogel capsules	299:343	alginate dialdehyde-gelatin hydrogel capsules	299:343	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	7	37	theme	45S5	1192:1195	arg1	BG					1197:1198	45S5 BG	1192:1198	45S5 BG	1192:1198	The results show that 45S5 BG can positively regulate the osteogenic activity of cells incorporated in hydrogel capsules.					
33405347	8	38	theme	cell	1358:1361	arg1	delivery					1363:1370	cell delivery	1358:1370	cell delivery	1358:1370	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	2	39	theme	gelation	414:421	arg1	time					423:426	the hydrogel gelation time	401:426	the hydrogel gelation time	401:426	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	1	40	theme	particle	224:231	arg1	microparticles					203:216	45S5 bioactive glass (BG) microparticles	177:216	45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	177:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	40	theme	particle	224:231	arg1	size					233:236	mean particle size ≈ 2 µm	219:243	mean particle size ≈ 2 µm	219:243	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	0	41	theme	Alginate	39:46	arg1	Microcapsules					67:79	Alginate Dialdehyde-Gelatin Microcapsules	39:79	Alginate Dialdehyde-Gelatin Microcapsules	39:79	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	1	42	theme	capsules	336:343	arg1	fabrication					253:263	fabrication	253:263	fabrication	253:263	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	42	theme	capsules	336:343	arg1	properties					285:294	physicochemical properties	269:294	physicochemical properties	269:294	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	5	43	theme	same	1039:1042	arg1	level					1044:1048	the same level	1035:1048	the same level	1035:1048	The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation.					
33405347	3	44	theme	w/v	763:765	arg1	concentrations					740:753	concentrations	740:753	concentrations of 0.1% w/v and 0.5% w/v, respectively	740:792	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	2	45	dep	%	487:487	arg1	BG					493:494	w/v BG	489:494	0.5% w/v BG	484:494	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	2	46	theme	w/v	489:491	arg1	BG					493:494	w/v BG	489:494	0.5% w/v BG	484:494	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	3	47	theme	hydrogel	566:573	arg1	capsules					575:582	hydrogel capsules	566:582	hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively	566:792	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	2	48	theme	BG	378:379	arg1	particles					381:389	BG particles	378:389	BG particles	378:389	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	0	49	theme	Microcapsules	67:79	arg1	Characterization					19:34	Characterization	19:34	Characterization	19:34	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	0	49	theme	Microcapsules	67:79	arg1	Biofabrication					0:13	Biofabrication	0:13	Biofabrication	0:13	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	3	50	theme	capsules	575:582	arg1	diameter					554:561	average diameter	546:561	average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively	546:792	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	6	51	theme	early	1082:1086	arg1	viability					1093:1101	The early cell viability	1078:1101	The early cell viability in samples containing BG	1078:1126	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	6	51	theme	early	1082:1086	arg1	lower					1131:1135	lower	1131:1135	lower	1131:1135	The early cell viability in samples containing BG is lower than that in samples without BG.					
33405347	3	52	theme	0.1	758:760	arg1	%					761:761	%	761:761	%	761:761	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	8	53	theme	regeneration	1380:1391	arg1	applications					1393:1404	bone regeneration applications	1375:1404	bone regeneration applications	1375:1404	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	3	54	theme	extrusion	615:623	arg1	technique					625:633	a pressure-driven extrusion technique	597:633	a pressure-driven extrusion technique	597:633	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	3	55	theme	%	774:774	arg1	w/v					776:778	0.5% w/v	771:778	0.5% w/v	771:778	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	8	56	theme	fabricated	1296:1305	arg1	capsules					1317:1324	The fabricated composite capsules	1292:1324	The fabricated composite capsules	1292:1324	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	1	57	dep	fabrication	253:263	arg1	the					249:251	the	249:251	the	249:251	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	8	58	theme	bone	1375:1378	arg1	applications					1393:1404	bone regeneration applications	1375:1404	bone regeneration applications	1375:1404	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	1	59	theme	incorporation	160:172	arg1	effect					146:151	The effect	142:151	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules	142:343	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	3	60	theme	w/v	776:778	arg1	concentrations					740:753	concentrations	740:753	concentrations of 0.1% w/v and 0.5% w/v, respectively	740:792	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	8	61	from	potential	1344:1352	arg1	applications					1393:1404	bone regeneration applications	1375:1404	bone regeneration applications	1375:1404	The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.					
33405347	2	62	theme	hydrogel	405:412	arg1	time					423:426	the hydrogel gelation time	401:426	the hydrogel gelation time	401:426	The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively.					
33405347	3	63	dep	1000 µm	646:652	arg1	1700					690:693	1700	690:693	1700	690:693	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	3	63	dep	1000 µm	646:652	arg1	to					681:682	to	681:682	to	681:682	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	3	63	dep	1000 µm	646:652	arg1	1900 µm					699:705	1900 µm	699:705	1900 µm	699:705	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	0	64	theme	Bioactive	95:103	arg1	Glass					105:109	Bioactive Glass	95:109	Bioactive Glass for Cell Delivery Application	95:139	Biofabrication and Characterization of Alginate Dialdehyde-Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application.					
33405347	3	65	theme	pressure-driven	599:613	arg1	technique					625:633	a pressure-driven extrusion technique	597:633	a pressure-driven extrusion technique	597:633	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33405347	1	66	theme	45S5	177:180	arg1	microparticles					203:216	45S5 bioactive glass (BG) microparticles	177:216	45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm)	177:244	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	1	66	theme	45S5	177:180	arg1	size					233:236	mean particle size ≈ 2 µm	219:243	mean particle size ≈ 2 µm	219:243	The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated.					
33405347	4	67	attach	presence	799:806	arg1	capsules					831:838	the capsules	827:838	the capsules	827:838	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	4	67	attach	presence	799:806	arg2	particles					814:822	BG particles	811:822	BG particles	811:822	The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials.					
33405347	3	68	theme	0.5	771:773	arg1	%					774:774	%	774:774	%	774:774	Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively.					
33593557	0	0	theme	peripheral	82:91	arg1	repair					106:111	peripheral nerve system repair	82:111	peripheral nerve system repair	82:111	TPP ionically cross-linked chitosan/PLGA microspheres for the delivery of NGF for peripheral nerve system repair.					
33593557	4	1	theme	concentration	719:731	arg1	increase					703:710	The increase	699:710	The increase of TPP concentration	699:731	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	1	2	theme	growth	146:151	arg1	NGF					161:163	NGF	161:163	NGF	161:163	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	1	2	theme	growth	146:151	arg1	factor					153:158	nerve growth factor	140:158	nerve growth factor (NGF)	140:164	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	5	3	theme	sustained	887:895	arg1	release					897:903	The sustained release	883:903	The sustained release of NGF	883:910	The sustained release of NGF could promote PC12 cells differentiation and neurite growth in vitro.					
33593557	3	4	theme	3.4	677:679	arg1	±					689:689	±	689:689	±	689:689	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	2	5	dep	%	437:437	arg1	to					439:440	to	439:440	to	439:440	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	1	6	theme	factor	153:158	arg1	release					129:135	the release	125:135	the release of nerve growth factor (NGF) in the injured peripheral nerve	125:196	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	6	7	with	comparison	995:1004	arg1	solution					1015:1022	NGF solution	1011:1022	NGF solution without microencapsulation	1011:1049	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	0	8	theme	system	99:104	arg1	repair					106:111	peripheral nerve system repair	82:111	peripheral nerve system repair	82:111	TPP ionically cross-linked chitosan/PLGA microspheres for the delivery of NGF for peripheral nerve system repair.					
33593557	5	9	theme	PC12	926:929	arg1	differentiation					937:951	PC12 cells differentiation	926:951	PC12 cells differentiation	926:951	The sustained release of NGF could promote PC12 cells differentiation and neurite growth in vitro.					
33593557	4	10	theme	TPP	715:717	arg1	concentration					719:731	TPP concentration	715:731	TPP concentration	715:731	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	0	11	theme	nerve	93:97	arg1	repair					106:111	peripheral nerve system repair	82:111	peripheral nerve system repair	82:111	TPP ionically cross-linked chitosan/PLGA microspheres for the delivery of NGF for peripheral nerve system repair.					
33593557	7	12	theme	TPP/Chitosan/PLGA-NGF	1226:1246	arg1	microspheres					1248:1259	TPP/Chitosan/PLGA-NGF microspheres	1226:1259	TPP/Chitosan/PLGA-NGF microspheres	1226:1259	These results demonstrate the feasibility of using TPP/Chitosan/PLGA-NGF microspheres for neural tissue repair.					
33593557	3	13	theme	FT-IR	467:471	arg1	analyses					473:480	FT-IR analyses	467:480	FT-IR analyses	467:480	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	3	14	from	matrix	598:603	arg1	embedded					577:584	embedded	577:584	embedded	577:584	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	3	15	theme	Zeta	448:451	arg1	potential					453:461	Zeta potential	448:461	Zeta potential	448:461	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	6	16	theme	sciatic	1096:1102	arg1	regeneration					1110:1121	sciatic nerve regeneration	1096:1121	sciatic nerve regeneration	1096:1121	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	4	17	theme	decreased	815:823	arg1	release					829:835	the decreased NGF release	811:835	the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days	811:880	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	3	18	theme	confocal	496:503	arg1	microscopy					505:514	confocal microscopy	496:514	confocal microscopy	496:514	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	6	19	theme	NGF	1011:1013	arg1	solution					1015:1022	NGF solution	1011:1022	NGF solution without microencapsulation	1011:1049	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	2	20	from	%	445:445	arg1	concentrations					415:428	TPP concentrations	411:428	TPP concentrations from 1 % to 10 %	411:445	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	4	21	theme	network	746:752	arg1	stability					754:762	the network stability	742:762	the network stability	742:762	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	2	22	theme	1	435:435	arg1	%					437:437	%	437:437	%	437:437	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	2	23	with	%	404:404	arg1	concentrations					415:428	TPP concentrations	411:428	TPP concentrations from 1 % to 10 %	411:445	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	1	24	from	release	129:135	arg1	nerve					192:196	the injured peripheral nerve	169:196	the injured peripheral nerve	169:196	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	3	25	theme	PLGA	554:557	arg1	microspheres					559:570	multiple NGF-loaded PLGA microspheres	534:570	multiple NGF-loaded PLGA microspheres	534:570	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	6	26	from	atrophy	1158:1164	arg1	rats					1169:1172	rats	1169:1172	rats	1169:1172	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	2	27	theme	%	388:388	arg1	%					404:404	83.4 ± 1.5 % to 72.1 ± 1.6 %	377:404	83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %	377:445	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	4	28	theme	NGF	825:827	arg1	release					829:835	the decreased NGF release	811:835	the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days	811:880	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	0	29	theme	chitosan/PLGA	27:39	arg1	microspheres					41:52	chitosan/PLGA microspheres	27:52	chitosan/PLGA microspheres	27:52	TPP ionically cross-linked chitosan/PLGA microspheres for the delivery of NGF for peripheral nerve system repair.					
33593557	5	30	theme	cells	931:935	arg1	differentiation					937:951	PC12 cells differentiation	926:951	PC12 cells differentiation	926:951	The sustained release of NGF could promote PC12 cells differentiation and neurite growth in vitro.					
33593557	6	31	theme	nerve	1104:1108	arg1	regeneration					1110:1121	sciatic nerve regeneration	1096:1121	sciatic nerve regeneration	1096:1121	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	3	32	theme	multiple	534:541	arg1	microspheres					559:570	multiple NGF-loaded PLGA microspheres	534:570	multiple NGF-loaded PLGA microspheres	534:570	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	3	33	from	embedded	577:584	arg1	matrix					598:603	chitosan matrix	589:603	chitosan matrix	589:603	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	2	34	theme	TPP	411:413	arg1	concentrations					415:428	TPP concentrations	411:428	TPP concentrations from 1 % to 10 %	411:445	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	5	35	theme	NGF	908:910	arg1	release					897:903	The sustained release	883:903	The sustained release of NGF	883:910	The sustained release of NGF could promote PC12 cells differentiation and neurite growth in vitro.					
33593557	3	36	theme	TPP/Chitosan/PLGA-NGF	623:643	arg1	microspheres					645:656	TPP/Chitosan/PLGA-NGF microspheres	623:656	TPP/Chitosan/PLGA-NGF microspheres	623:656	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	3	37	theme	NGF-loaded	543:552	arg1	microspheres					559:570	multiple NGF-loaded PLGA microspheres	534:570	multiple NGF-loaded PLGA microspheres	534:570	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	1	38	theme	injured	173:179	arg1	nerve					192:196	the injured peripheral nerve	169:196	the injured peripheral nerve	169:196	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	3	39	dep	±	689:689	arg1	to					681:682	to	681:682	to	681:682	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	3	40	theme	microspheres	645:656	arg1	size					615:618	the mean size	606:618	the mean size of TPP/Chitosan/PLGA-NGF microspheres	606:656	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	1	41	theme	peripheral	181:190	arg1	nerve					192:196	the injured peripheral nerve	169:196	the injured peripheral nerve	169:196	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	2	42	theme	10	442:443	arg1	%					437:437	%	437:437	%	437:437	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	4	43	from	%	869:869	arg1	days					877:880	49 days	874:880	49 days	874:880	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	3	44	theme	chitosan	589:596	arg1	matrix					598:603	chitosan matrix	589:603	chitosan matrix	589:603	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	7	45	theme	tissue	1272:1277	arg1	repair					1279:1284	neural tissue repair	1265:1284	neural tissue repair	1265:1284	These results demonstrate the feasibility of using TPP/Chitosan/PLGA-NGF microspheres for neural tissue repair.					
33593557	2	46	theme	±	398:398	arg1	%					404:404	83.4 ± 1.5 % to 72.1 ± 1.6 %	377:404	83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %	377:445	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	3	47	theme	3.1	691:693	arg1	±					689:689	±	689:689	±	689:689	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	2	48	theme	NGF	361:363	arg1	efficiency					347:356	The encapsulation efficiency	329:356	The encapsulation efficiency of NGF	329:363	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	2	49	dep	%	404:404	arg1	to					390:391	to	390:391	to	390:391	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	4	50	theme	±	863:863	arg1	%					869:869	45.7 ± 0.8 %	858:869	45.7 ± 0.8 % in 49 days	858:880	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	3	51	theme	±	675:675	arg1	μm					695:696	40.2 ± 3.4 to 49.3 ± 3.1 μm	670:696	40.2 ± 3.4 to 49.3 ± 3.1 μm	670:696	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	5	52	theme	neurite	957:963	arg1	growth					965:970	neurite growth	957:970	neurite growth	957:970	The sustained release of NGF could promote PC12 cells differentiation and neurite growth in vitro.					
33593557	7	53	theme	neural	1265:1270	arg1	repair					1279:1284	neural tissue repair	1265:1284	neural tissue repair	1265:1284	These results demonstrate the feasibility of using TPP/Chitosan/PLGA-NGF microspheres for neural tissue repair.					
33593557	3	54	dep	together	482:489	arg1	with					491:494	with	491:494	with	491:494	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	6	55	theme	gastrocnemius	1137:1149	arg1	atrophy					1158:1164	gastrocnemius muscle atrophy	1137:1164	gastrocnemius muscle atrophy in rats	1137:1172	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	3	56	theme	mean	610:613	arg1	size					615:618	the mean size	606:618	the mean size of TPP/Chitosan/PLGA-NGF microspheres	606:656	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	2	57	theme	encapsulation	333:345	arg1	efficiency					347:356	The encapsulation efficiency	329:356	The encapsulation efficiency of NGF	329:363	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	1	58	theme	NGF-loaded	199:208	arg1	microspheres					234:245	NGF-loaded chitosan/PLGA composite microspheres	199:245	NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF)	199:312	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	0	59	theme	NGF	74:76	arg1	delivery					62:69	the delivery	58:69	the delivery of NGF for peripheral nerve system repair	58:111	TPP ionically cross-linked chitosan/PLGA microspheres for the delivery of NGF for peripheral nerve system repair.					
33593557	4	60	from	%	853:853	arg1	release					829:835	the decreased NGF release	811:835	the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days	811:880	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	4	61	theme	swelling	782:789	arg1	ratio					791:795	the swelling ratio	778:795	the swelling ratio	778:795	The increase of TPP concentration improved the network stability and decreased the swelling ratio, resulting in the decreased NGF release from 67.7 ± 1.2 % to 45.7 ± 0.8 % in 49 days.					
33593557	2	62	theme	±	382:382	arg1	%					404:404	83.4 ± 1.5 % to 72.1 ± 1.6 %	377:404	83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %	377:445	The encapsulation efficiency of NGF ranged from 83.4 ± 1.5 % to 72.1 ± 1.6 % with TPP concentrations from 1 % to 10 %.					
33593557	6	63	theme	muscle	1151:1156	arg1	atrophy					1158:1164	gastrocnemius muscle atrophy	1137:1164	gastrocnemius muscle atrophy in rats	1137:1172	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	1	64	theme	chitosan/PLGA	210:222	arg1	microspheres					234:245	NGF-loaded chitosan/PLGA composite microspheres	199:245	NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF)	199:312	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	3	65	theme	49.3	684:687	arg1	±					689:689	±	689:689	±	689:689	Zeta potential and FT-IR analyses together with confocal microscopy demonstrated that multiple NGF-loaded PLGA microspheres were embedded in chitosan matrix, the mean size of TPP/Chitosan/PLGA-NGF microspheres ranged from 40.2 ± 3.4 to 49.3 ± 3.1 μm.					
33593557	6	66	theme	TPP/Chitosan/PLGA-NGF	1052:1072	arg1	microspheres					1074:1085	TPP/Chitosan/PLGA-NGF microspheres	1052:1085	TPP/Chitosan/PLGA-NGF microspheres	1052:1085	Moreover, in comparison with NGF solution without microencapsulation, TPP/Chitosan/PLGA-NGF microspheres enhanced sciatic nerve regeneration and prevented gastrocnemius muscle atrophy in rats.					
33593557	1	67	theme	nerve	140:144	arg1	NGF					161:163	NGF	161:163	NGF	161:163	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	1	67	theme	nerve	140:144	arg1	factor					153:158	nerve growth factor	140:158	nerve growth factor (NGF)	140:164	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33593557	1	68	theme	composite	224:232	arg1	microspheres					234:245	NGF-loaded chitosan/PLGA composite microspheres	199:245	NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF)	199:312	To control the release of nerve growth factor (NGF) in the injured peripheral nerve, NGF-loaded chitosan/PLGA composite microspheres ionically cross-linked by tripolyphosphate (TPP/Chitosan/PLGA-NGF) were prepared.					
33826065	2	0	theme	methanol	580:587	arg1	recycling					606:614	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	4	1	theme	S	1053:1053	arg1	-CPMA					1055:1059	(S)-CPMA	1052:1059	(S)-CPMA	1052:1059	With simplified selective adsorption and extraction procedures, (S)-CPMA was obtained in 84% isolated yield.					
33826065	0	2	theme	Continuous	89:98	arg1	Production					100:109	Continuous Production	89:109	Continuous Production of Chiral Diaryl Alcohol	89:134	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	3	3	theme	substrate	954:962	arg1	concentration					964:976	substrate concentration	954:976	substrate concentration of 10 mM	954:985	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	2	4	theme	recycling	606:614	arg1	synthesis					507:515	the continuous synthesis	492:515	the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	492:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	1	5	theme	Ni-NTA	197:202	arg1	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	5	theme	Ni-NTA	197:202	arg1	Cerose					204:209	Ni-NTA Cerose	197:209	Ni-NTA Cerose	197:209	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	3	6	from	rate	933:936	arg1	days					918:921	the first three days	902:921	the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM	902:985	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	1	7	theme	metal	279:283	arg1	tag					294:296	a metal affinity tag	277:296	a metal affinity tag	277:296	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	0	8	theme	Chiral	114:119	arg1	Alcohol					128:134	Chiral Diaryl Alcohol	114:134	Chiral Diaryl Alcohol	114:134	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	2	9	theme	pyridin-2-yl	566:577	arg1	-CPMA					593:597	(S)-CPMA	590:597	(S)-CPMA	590:597	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	9	theme	pyridin-2-yl	566:577	arg1	methanol					580:587	(pyridin-2-yl) methanol	565:587	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	10	theme	NADPH	600:604	arg1	recycling					606:614	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	1	11	theme	affinity	285:292	arg1	tag					294:296	a metal affinity tag	277:296	a metal affinity tag	277:296	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	2	12	theme	chiral	524:529	arg1	S					545:545	S	545:545	S	545:545	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	12	theme	chiral	524:529	arg1	intermediate					531:542	the chiral intermediate	520:542	the chiral intermediate (S)	520:546	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	3	13	theme	mL/min	943:948	arg1	rate					933:936	a flow rate	926:936	a flow rate of 5 mL/min	926:948	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	3	13	theme	mL/min	943:948	arg1	concentration					964:976	substrate concentration	954:976	substrate concentration of 10 mM	954:985	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	3	14	theme	first	906:910	arg1	days					918:921	the first three days	902:921	the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM	902:985	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	4	15	theme	extraction	1029:1038	arg1	procedures					1040:1049	extraction procedures	1029:1049	extraction procedures	1029:1049	With simplified selective adsorption and extraction procedures, (S)-CPMA was obtained in 84% isolated yield.					
33826065	0	16	theme	Alcohol	128:134	arg1	Production					100:109	Continuous Production	89:109	Continuous Production of Chiral Diaryl Alcohol	89:134	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	3	17	dep	Mu-S5	784:788	arg1	BmGDH					791:795	BmGDH	791:795	Mu-S5: BmGDH	784:795	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	2	18	theme	resin	709:713	arg1	column					715:720	a D101 macroporous resin column	690:720	a D101 macroporous resin column	690:720	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	3	19	theme	activity	761:768	arg1	ratio					770:774	an optimum enzyme activity ratio	743:774	an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH)	743:796	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	0	20	theme	Diaryl	121:126	arg1	Alcohol					128:134	Chiral Diaryl Alcohol	114:134	Chiral Diaryl Alcohol	114:134	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	2	21	theme	S	591:591	arg1	-CPMA					593:597	(S)-CPMA	590:597	(S)-CPMA	590:597	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	21	theme	S	591:591	arg1	methanol					580:587	(pyridin-2-yl) methanol	565:587	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	22	theme	macroporous	697:707	arg1	column					715:720	a D101 macroporous resin column	690:720	a D101 macroporous resin column	690:720	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	0	23	theme	Alcohol	15:21	arg1	Dehydrogenase					23:35	Alcohol Dehydrogenase	15:35	Alcohol Dehydrogenase	15:35	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	2	24	theme	in	621:622	arg1	adsorption					637:646	in situ product adsorption	621:646	in situ product adsorption	621:646	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	25	theme	dehydrogenase	332:344	arg1	Mu-S5					346:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	26	theme	D101	692:695	arg1	column					715:720	a D101 macroporous resin column	690:720	a D101 macroporous resin column	690:720	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	27	theme	packed	463:468	arg1	PBR					483:485	PBR	483:485	PBR	483:485	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	27	theme	packed	463:468	arg1	reactor					474:480	a packed bed reactor	461:480	a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	461:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	3	28	from	concentration	964:976	arg1	days					918:921	the first three days	902:921	the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM	902:985	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	2	29	theme	alcohol	324:330	arg1	Mu-S5					346:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	4	30	theme	isolated	1081:1088	arg1	yield					1090:1094	84% isolated yield	1077:1094	84% isolated yield	1077:1094	With simplified selective adsorption and extraction procedures, (S)-CPMA was obtained in 84% isolated yield.					
33826065	2	31	from	megaterium	392:401	arg1	Mu-S5					346:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	31	from	megaterium	392:401	arg1	dehydrogenase					364:376	glucose dehydrogenase	356:376	glucose dehydrogenase from Bacillus megaterium (BmGDH)	356:409	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	32	theme	fusion	317:322	arg1	Mu-S5					346:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	3	33	theme	g/	873:874	arg1	yield					859:863	a space-time yield	846:863	a space-time yield of 1560 g/(L·d)	846:879	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	2	34	theme	6×His-tag	307:315	arg1	Mu-S5					346:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	a 6×His-tag fusion alcohol dehydrogenase Mu-S5	305:350	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	4	35	theme	selective	1004:1012	arg1	adsorption					1014:1023	simplified selective adsorption	993:1023	simplified selective adsorption	993:1023	With simplified selective adsorption and extraction procedures, (S)-CPMA was obtained in 84% isolated yield.					
33826065	2	36	theme	glucose	356:362	arg1	dehydrogenase					364:376	glucose dehydrogenase	356:376	glucose dehydrogenase from Bacillus megaterium (BmGDH)	356:409	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	37	theme	Ni-NTA	434:439	arg1	Cerose					441:446	Ni-NTA Cerose	434:446	Ni-NTA Cerose	434:446	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	4	38	theme	simplified	993:1002	arg1	adsorption					1014:1023	simplified selective adsorption	993:1023	simplified selective adsorption	993:1023	With simplified selective adsorption and extraction procedures, (S)-CPMA was obtained in 84% isolated yield.					
33826065	1	39	theme	carrier	227:233	arg1	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	39	theme	carrier	227:233	arg1	materials					235:243	carrier materials	227:243	carrier materials	227:243	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	0	40	theme	Glucose	41:47	arg1	Dehydrogenase					49:61	Glucose Dehydrogenase	41:61	Glucose Dehydrogenase	41:61	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	3	41	theme	2:1	779:781	arg1	ratio					770:774	an optimum enzyme activity ratio	743:774	an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH)	743:796	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	3	41	theme	2:1	779:781	arg1	hydroxypropyl-β-cyclodextrin					802:829	hydroxypropyl-β-cyclodextrin	802:829	hydroxypropyl-β-cyclodextrin	802:829	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	3	42	theme	space-time	848:857	arg1	yield					859:863	a space-time yield	846:863	a space-time yield of 1560 g/(L·d)	846:879	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	0	43	theme	Resin	68:72	arg1	Extraction					74:83	Resin Extraction	68:83	Resin Extraction for Continuous Production of Chiral Diaryl Alcohol	68:134	Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol.					
33826065	2	44	theme	intermediate	531:542	arg1	synthesis					507:515	the continuous synthesis	492:515	the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	492:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	3	45	theme	mM	984:985	arg1	rate					933:936	a flow rate	926:936	a flow rate of 5 mL/min	926:948	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	3	45	theme	mM	984:985	arg1	concentration					964:976	substrate concentration	954:976	substrate concentration of 10 mM	954:985	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	1	46	used	used	219:222	arg2	materials					235:243	carrier materials	227:243	carrier materials	227:243	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	46	used	used	219:222	arg2	Cerose					204:209	Ni-NTA Cerose	197:209	Ni-NTA Cerose	197:209	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	46	used	used	219:222	arg2	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	47	theme	Ni2+-functionalized	137:155	arg1	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	47	theme	Ni2+-functionalized	137:155	arg1	Cerose					204:209	Ni-NTA Cerose	197:209	Ni-NTA Cerose	197:209	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	47	theme	Ni2+-functionalized	137:155	arg1	materials					235:243	carrier materials	227:243	carrier materials	227:243	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	3	48	theme	flow	928:931	arg1	rate					933:936	a flow rate	926:936	a flow rate of 5 mL/min	926:948	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	2	49	theme	4-chlorophenyl	549:562	arg1	recycling					606:614	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	548:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	3	50	dep	ratio	770:774	arg1	Mu-S5					784:788	Mu-S5	784:788	Mu-S5: BmGDH	784:795	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	2	51	theme	product	629:635	arg1	adsorption					637:646	in situ product adsorption	621:646	in situ product adsorption	621:646	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	1	52	theme	porous	157:162	arg1	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	52	theme	porous	157:162	arg1	Cerose					204:209	Ni-NTA Cerose	197:209	Ni-NTA Cerose	197:209	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	52	theme	porous	157:162	arg1	materials					235:243	carrier materials	227:243	carrier materials	227:243	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	4	53	dep	isolated	1081:1088	arg1	%					1079:1079	84%	1077:1079	84%	1077:1079	With simplified selective adsorption and extraction procedures, (S)-CPMA was obtained in 84% isolated yield.					
33826065	3	54	theme	optimum	746:752	arg1	ratio					770:774	an optimum enzyme activity ratio	743:774	an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH)	743:796	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	1	55	theme	ceramic/agarose	164:178	arg1	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	55	theme	ceramic/agarose	164:178	arg1	Cerose					204:209	Ni-NTA Cerose	197:209	Ni-NTA Cerose	197:209	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	55	theme	ceramic/agarose	164:178	arg1	materials					235:243	carrier materials	227:243	carrier materials	227:243	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	3	56	theme	enzyme	754:759	arg1	ratio					770:774	an optimum enzyme activity ratio	743:774	an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH)	743:796	Using an optimum enzyme activity ratio of 2:1 (Mu-S5: BmGDH) and hydroxypropyl-β-cyclodextrin as co-solvent, a space-time yield of 1560 g/(L·d) could be achieved in the first three days at a flow rate of 5 mL/min and substrate concentration of 10 mM.					
33826065	1	57	theme	composite	180:188	arg1	beads					190:194	Ni2+-functionalized porous ceramic/agarose composite beads	137:194	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose)	137:210	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	57	theme	composite	180:188	arg1	Cerose					204:209	Ni-NTA Cerose	197:209	Ni-NTA Cerose	197:209	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	1	57	theme	composite	180:188	arg1	materials					235:243	carrier materials	227:243	carrier materials	227:243	Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag.					
33826065	2	58	dep	in	621:622	arg1	situ					624:627	situ	624:627	situ	624:627	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	59	theme	continuous	496:505	arg1	synthesis					507:515	the continuous synthesis	492:515	the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	492:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	60	theme	bed	470:472	arg1	PBR					483:485	PBR	483:485	PBR	483:485	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
33826065	2	60	theme	bed	470:472	arg1	reactor					474:480	a packed bed reactor	461:480	a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling	461:614	Here, a 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose dehydrogenase from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose to construct a packed bed reactor (PBR) for the continuous synthesis of the chiral intermediate (S)-(4-chlorophenyl)-(pyridin-2-yl) methanol ((S)-CPMA) NADPH recycling, and in situ product adsorption was achieved simultaneously by assembling a D101 macroporous resin column after the PBR.					
32622189	0	0	theme	storage	86:92	arg1	quality					94:100	the storage quality	82:100	the storage quality of raw meat	82:112	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs to maintain the storage quality of raw meat.					
32622189	5	1	theme	hydrogen	803:810	arg1	force					832:836	the main force	823:836	the main force	823:836	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	5	1	theme	hydrogen	803:810	arg1	bonds					812:816	hydrogen bonds	803:816	hydrogen bonds	803:816	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	1	2	theme	protein/linseed	157:171	arg1	NPs					181:183	chitosan/potato protein/linseed oil/ZnO NPs	141:183	chitosan/potato protein/linseed oil/ZnO NPs	141:183	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	5	3	theme	FTIR	667:670	arg1	analysis					672:679	FTIR analysis	667:679	FTIR analysis	667:679	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	1	4	theme	oil/ZnO	173:179	arg1	NPs					181:183	chitosan/potato protein/linseed oil/ZnO NPs	141:183	chitosan/potato protein/linseed oil/ZnO NPs	141:183	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	7	5	theme	bacterial	1145:1153	arg1	counts					1155:1160	total bacterial counts	1139:1160	total bacterial counts	1139:1160	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	6	6	theme	meat	843:846	arg1	samples					848:854	Raw meat samples	839:854	Raw meat samples	839:854	Raw meat samples were wrapped with different films to evaluate the preservative effect during 4 °C storage.					
32622189	2	7	theme	NPs	293:295	arg1	incorporation					272:284	the incorporation	268:284	the incorporation of ZnO NPs	268:295	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	4	8	theme	ZnO	606:608	arg1	NPs					610:612	ZnO NPs	606:612	ZnO NPs	606:612	SEM showed that ZnO NPs could harmoniously exist in various polymers matrix.					
32622189	5	9	theme	intermolecular	762:775	arg1	interactions					777:788	intramolecular and intermolecular interactions	743:788	intramolecular and intermolecular interactions	743:788	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	3	10	theme	excellent	551:559	arg1	capability					578:587	an excellent moisture barrier capability	548:587	an excellent moisture barrier capability	548:587	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	6	11	theme	Raw	839:841	arg1	samples					848:854	Raw meat samples	839:854	Raw meat samples	839:854	Raw meat samples were wrapped with different films to evaluate the preservative effect during 4 °C storage.					
32622189	2	12	theme	ZnO	289:291	arg1	NPs					293:295	ZnO NPs	289:295	ZnO NPs	289:295	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	0	13	theme	meat	109:112	arg1	quality					94:100	the storage quality	82:100	the storage quality of raw meat	82:112	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs to maintain the storage quality of raw meat.					
32622189	2	14	theme	linseed	393:399	arg1	oil					401:403	linseed oil	393:403	linseed oil	393:403	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	0	15	theme	raw	105:107	arg1	meat					109:112	raw meat	105:112	raw meat	105:112	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs to maintain the storage quality of raw meat.					
32622189	7	16	theme	total	1139:1143	arg1	counts					1155:1160	total bacterial counts	1139:1160	total bacterial counts	1139:1160	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	2	17	theme	good	444:447	arg1	property					457:464	good elastic property	444:464	good elastic property	444:464	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	6	18	theme	preservative	906:917	arg1	effect					919:924	the preservative effect	902:924	the preservative effect	902:924	Raw meat samples were wrapped with different films to evaluate the preservative effect during 4 °C storage.					
32622189	7	19	theme	best	986:989	arg1	effect					1002:1007	best protective effect	986:1007	best protective effect of raw meat with excellent acceptable sensory properties	986:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	0	20	theme	Biopolymer	0:9	arg1	films					11:15	Biopolymer films	0:15	Biopolymer films based on chitosan/potato	0:40	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs to maintain the storage quality of raw meat.					
32622189	2	21	dep	transparency	327:338	arg1	the					323:325	the	323:325	the	323:325	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	0	22	theme	oil/ZnO	58:64	arg1	NPs					66:68	oil/ZnO NPs	58:68	oil/ZnO NPs	58:68	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs to maintain the storage quality of raw meat.					
32622189	7	23	theme	protective	991:1000	arg1	effect					1002:1007	best protective effect	986:1007	best protective effect of raw meat with excellent acceptable sensory properties	986:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	3	24	theme	moisture	561:568	arg1	capability					578:587	an excellent moisture barrier capability	548:587	an excellent moisture barrier capability	548:587	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	4	25	theme	various	642:648	arg1	polymers					650:657	various polymers	642:657	various polymers matrix	642:664	SEM showed that ZnO NPs could harmoniously exist in various polymers matrix.					
32622189	2	26	theme	composite	420:428	arg1	film					430:433	the composite film	416:433	the composite film	416:433	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	3	27	theme	barrier	570:576	arg1	capability					578:587	an excellent moisture barrier capability	548:587	an excellent moisture barrier capability	548:587	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	3	28	theme	oil/ZnO	518:524	arg1	NPs					526:528	chitosan/potato protein/linseed oil/ZnO NPs	486:528	chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl)	486:536	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	3	28	theme	oil/ZnO	518:524	arg1	Fcpzl					531:535	Fcpzl	531:535	Fcpzl	531:535	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	2	29	theme	tensile	344:350	arg1	strength					352:359	tensile strength	344:359	tensile strength	344:359	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	1	30	theme	storage	216:222	arg1	quality					224:230	the storage quality	212:230	the storage quality of raw meat	212:242	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	5	31	theme	intramolecular	743:756	arg1	interactions					777:788	intramolecular and intermolecular interactions	743:788	intramolecular and intermolecular interactions	743:788	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	2	32	theme	oil	401:403	arg1	addition					381:388	addition	381:388	addition of linseed oil	381:403	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	5	33	theme	main	827:830	arg1	force					832:836	the main force	823:836	the main force	823:836	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	5	33	theme	main	827:830	arg1	bonds					812:816	hydrogen bonds	803:816	hydrogen bonds	803:816	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	7	34	with	effect	1002:1007	arg1	properties					1055:1064	excellent acceptable sensory properties	1026:1064	excellent acceptable sensory properties	1026:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	7	35	theme	7 days	1073:1078	arg1	storage					1080:1086	7 days storage	1073:1086	7 days storage	1073:1086	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	3	36	theme	chitosan/potato	486:500	arg1	NPs					526:528	chitosan/potato protein/linseed oil/ZnO NPs	486:528	chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl)	486:536	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	3	36	theme	chitosan/potato	486:500	arg1	Fcpzl					531:535	Fcpzl	531:535	Fcpzl	531:535	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	2	37	theme	elastic	449:455	arg1	property					457:464	good elastic property	444:464	good elastic property	444:464	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	5	38	theme	different	699:707	arg1	components					709:718	different components	699:718	different components	699:718	FTIR analysis demonstrated that different components were bound together by intramolecular and intermolecular interactions, among which hydrogen bonds were the main force.					
32622189	6	39	theme	different	874:882	arg1	films					884:888	different films	874:888	different films	874:888	Raw meat samples were wrapped with different films to evaluate the preservative effect during 4 °C storage.					
32622189	7	40	theme	sensory	1047:1053	arg1	properties					1055:1064	excellent acceptable sensory properties	1026:1064	excellent acceptable sensory properties	1026:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	1	41	theme	Biopolymer	115:124	arg1	films					126:130	Biopolymer films	115:130	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs	115:183	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	7	42	theme	raw	1012:1014	arg1	meat					1016:1019	raw meat	1012:1019	raw meat	1012:1019	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	7	43	theme	excellent	1026:1034	arg1	properties					1055:1064	excellent acceptable sensory properties	1026:1064	excellent acceptable sensory properties	1026:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	1	44	theme	chitosan/potato	141:155	arg1	NPs					181:183	chitosan/potato protein/linseed oil/ZnO NPs	141:183	chitosan/potato protein/linseed oil/ZnO NPs	141:183	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	1	45	theme	raw	235:237	arg1	meat					239:242	raw meat	235:242	raw meat	235:242	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	4	46	theme	polymers	650:657	arg1	matrix					659:664	various polymers matrix	642:664	various polymers matrix	642:664	SEM showed that ZnO NPs could harmoniously exist in various polymers matrix.					
32622189	7	47	theme	acceptable	1036:1045	arg1	properties					1055:1064	excellent acceptable sensory properties	1026:1064	excellent acceptable sensory properties	1026:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	1	48	theme	meat	239:242	arg1	quality					224:230	the storage quality	212:230	the storage quality of raw meat	212:242	Biopolymer films based on chitosan/potato protein/linseed oil/ZnO NPs were developed to maintain the storage quality of raw meat.					
32622189	7	49	contain	possessed	976:984	arg2	effect					1002:1007	best protective effect	986:1007	best protective effect of raw meat with excellent acceptable sensory properties	986:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	7	49	contain	possessed	976:984	arg1	Fcpzl					970:974	Fcpzl	970:974	Fcpzl	970:974	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	7	50	theme	meat	1016:1019	arg1	effect					1002:1007	best protective effect	986:1007	best protective effect of raw meat with excellent acceptable sensory properties	986:1064	Results indicated that Fcpzl possessed best protective effect of raw meat with excellent acceptable sensory properties during 7 days storage, which could reduce the speed of increasing pH and total bacterial counts.					
32622189	2	51	theme	films	368:372	arg1	transparency					327:338	transparency	327:338	transparency	327:338	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	2	51	theme	films	368:372	arg1	strength					352:359	tensile strength	344:359	tensile strength	344:359	Results indicated that the incorporation of ZnO NPs could effectively improve the transparency and tensile strength of the films, while addition of linseed oil could make the composite film maintain good elastic property.					
32622189	3	52	theme	protein/linseed	502:516	arg1	NPs					526:528	chitosan/potato protein/linseed oil/ZnO NPs	486:528	chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl)	486:536	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32622189	3	52	theme	protein/linseed	502:516	arg1	Fcpzl					531:535	Fcpzl	531:535	Fcpzl	531:535	Films blended with chitosan/potato protein/linseed oil/ZnO NPs (Fcpzl) exhibited an excellent moisture barrier capability.					
32540066	10	0	theme	Lux	2156:2158	arg1	Cellulose-1					2160:2170	Lux Cellulose-1	2156:2170	Lux Cellulose-1	2156:2170	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	5	1	dep	view	1174:1177	arg1	contrast					1140:1147	contrast	1140:1147	contrast	1140:1147	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	6	2	theme	CSs	1381:1383	arg1	similar					1388:1394	similar	1388:1394	similar	1388:1394	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	2	theme	CSs	1381:1383	arg1	behavior					1363:1370	the behavior	1359:1370	the behavior of these CSs	1359:1383	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	3	theme	content	1311:1317	arg1	range					1296:1300	the range	1292:1300	the range of water content in the mobile phase of up to 20-30% v/v	1292:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	9	4	theme	pure	1974:1977	arg1	mode					2001:2004	pure polar-organic solvent mode	1974:2004	pure polar-organic solvent mode	1974:2004	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	10	5	theme	enantiomer	2031:2040	arg1	reversal					2062:2069	enantiomer elution order (EEO) reversal	2031:2069	enantiomer elution order (EEO) reversal	2031:2069	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	0	6	theme	substituent	213:223	arg1	effects					262:268	substituent electron-donor and electron-acceptor effects	213:268	substituent electron-donor and electron-acceptor effects	213:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	1	7	theme	mobile	522:527	arg1	phases					529:534	aqueous-acetonitrile mobile phases	501:534	aqueous-acetonitrile mobile phases in HPLC	501:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	1	8	theme	chiral	331:336	arg1	columns					338:344	amylose- and cellulose-phenylcarbamate-based chiral columns	286:344	amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries	286:392	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	9	9	theme	solvent	1993:1999	arg1	mode					2001:2004	pure polar-organic solvent mode	1974:2004	pure polar-organic solvent mode	1974:2004	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	9	10	theme	composition	1881:1891	arg1	HILIC-range					1853:1863	the HILIC-range	1849:1863	the HILIC-range	1849:1863	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	9	10	theme	composition	1881:1891	arg1	advantageous					1956:1967	advantageous	1956:1967	advantageous	1956:1967	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	0	11	theme	liquid	175:180	arg1	chromatography					182:195	high-performance liquid chromatography	158:195	high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects	158:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	5	12	with	agreement	1087:1095	arg1	results					1110:1116	earlier results	1102:1116	earlier results	1102:1116	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	10	13	theme	EEO	2057:2059	arg1	reversal					2062:2069	enantiomer elution order (EEO) reversal	2031:2069	enantiomer elution order (EEO) reversal	2031:2069	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	1	14	theme	chiral	465:470	arg1	analytes					472:479	basic chiral analytes	459:479	basic chiral analytes in acetonitrile	459:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	4	15	theme	aqueous	1051:1057	arg1	acetonitrile					1059:1070	aqueous acetonitrile	1051:1070	aqueous acetonitrile	1051:1070	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	3	16	theme	chiral	866:871	arg1	analytes					873:880	chiral analytes	866:880	chiral analytes	866:880	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	5	17	theme	polysaccharide	1180:1193	arg1	phenylcarbamates					1195:1210	polysaccharide phenylcarbamates	1180:1210	polysaccharide phenylcarbamates	1180:1210	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	3	18	theme	amylose	897:903	arg1	case					889:892	the case	885:892	the case of amylose and cellulose derivatives	885:929	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	6	19	theme	liquid	1423:1428	arg1	chromatography					1430:1443	hydrophilic interaction liquid chromatography	1399:1443	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	19	theme	liquid	1423:1428	arg1	HILIC					1446:1450	HILIC	1446:1450	HILIC	1446:1450	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	4	20	theme	phenylcarbamates	1011:1026	arg1	behavior					984:991	the behavior	980:991	the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents	980:1081	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	10	21	theme	reversal	2062:2069	arg1	examples					2019:2026	Interesting examples	2007:2026	Interesting examples of enantiomer elution order (EEO) reversal	2007:2069	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	6	22	theme	hydrophilic	1399:1409	arg1	chromatography					1430:1443	hydrophilic interaction liquid chromatography	1399:1443	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	22	theme	hydrophilic	1399:1409	arg1	HILIC					1446:1450	HILIC	1446:1450	HILIC	1446:1450	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	4	23	from	acetonitrile	1031:1042	arg1	behavior					984:991	the behavior	980:991	the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents	980:1081	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	3	24	theme	CSs	661:663	arg1	comparison					647:656	The comparison	643:656	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents	643:817	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	10	25	theme	Amylose-2	2180:2188	arg1	columns					2190:2196	Lux Amylose-2 columns	2176:2196	Lux Amylose-2 columns	2176:2196	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	4	26	from	phenylcarbamates	1011:1026	arg1	acetonitrile					1031:1042	acetonitrile	1031:1042	acetonitrile	1031:1042	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	4	26	from	phenylcarbamates	1011:1026	arg1	acetonitrile					1059:1070	aqueous acetonitrile	1051:1070	aqueous acetonitrile	1051:1070	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	7	27	from	content	1508:1514	arg1	phase					1530:1534	the mobile phase up to 20-30% v/v	1519:1551	the mobile phase up to 20-30% v/v	1519:1551	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	2	28	theme	amylose-based	569:581	arg1	columns					583:589	the amylose-based columns	565:589	the amylose-based columns with coated and immobilized CSs	565:621	For two chemistries the amylose-based columns with coated and immobilized CSs were also compared.					
32540066	3	29	theme	cellulose	909:917	arg1	derivatives					919:929	cellulose derivatives	909:929	cellulose derivatives	909:929	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	1	30	from	acetonitrile	484:495	arg1	phases					529:534	aqueous-acetonitrile mobile phases	501:534	aqueous-acetonitrile mobile phases in HPLC	501:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	1	30	from	acetonitrile	484:495	arg1	enantiomers					444:454	enantiomers	444:454	enantiomers of basic chiral analytes in acetonitrile	444:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	3	31	with	comparison	647:656	arg1	those					741:745	those	741:745	those	741:745	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	6	32	dep	20-30	1348:1352	arg1	to					1345:1346	to	1345:1346	to	1345:1346	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	0	33	theme	cellulose-	66:75	arg1	columns					106:112	amylose- and cellulose- phenylcarbamate-based chiral columns	53:112	amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	53:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	5	34	theme	basic	1266:1270	arg1	analytes					1272:1279	basic analytes	1266:1279	basic analytes	1266:1279	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	0	35	from	Separation	0:9	arg1	chromatography					182:195	high-performance liquid chromatography	158:195	high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects	158:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	0	36	theme	chiral	99:104	arg1	columns					106:112	amylose- and cellulose- phenylcarbamate-based chiral columns	53:112	amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	53:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	5	37	theme	earlier	1102:1108	arg1	results					1110:1116	earlier results	1102:1116	earlier results	1102:1116	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	6	38	from	range	1296:1300	arg1	similar					1388:1394	similar	1388:1394	similar	1388:1394	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	38	from	range	1296:1300	arg1	phase					1333:1337	the mobile phase	1322:1337	the mobile phase of up to 20-30% v/v	1322:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	38	from	range	1296:1300	arg1	behavior					1363:1370	the behavior	1359:1370	the behavior of these CSs	1359:1383	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	1	39	from	enantiomers	444:454	arg1	HPLC					539:542	HPLC	539:542	HPLC	539:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	1	39	from	enantiomers	444:454	arg1	acetonitrile					484:495	acetonitrile	484:495	acetonitrile	484:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	5	40	theme	typical	1229:1235	arg1	materials					1252:1260	typical reversed-phase materials	1229:1260	typical reversed-phase materials for basic analytes	1229:1279	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	9	41	theme	phase	1875:1879	arg1	composition					1881:1891	mobile phase composition	1868:1891	mobile phase composition	1868:1891	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	1	42	from	separation	430:439	arg1	HPLC					539:542	HPLC	539:542	HPLC	539:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	0	43	theme	enantiomers	14:24	arg1	Separation					0:9	Separation	0:9	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.	0:269	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	1	44	theme	different	351:359	arg1	chemistries					382:392	different chiral-selector (CS) chemistries	351:392	different chiral-selector (CS) chemistries	351:392	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	9	45	theme	shorter	1903:1909	arg1	time					1920:1923	shorter analysis time	1903:1923	shorter analysis time	1903:1923	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	0	46	from	focus	204:208	arg1	effects					262:268	substituent electron-donor and electron-acceptor effects	213:268	substituent electron-donor and electron-acceptor effects	213:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	0	47	theme	chiral	29:34	arg1	drugs					42:46	chiral basic drugs	29:46	chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	29:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	0	48	theme	amylose-	53:60	arg1	columns					106:112	amylose- and cellulose- phenylcarbamate-based chiral columns	53:112	amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	53:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	0	49	theme	drugs	42:46	arg1	enantiomers					14:24	enantiomers	14:24	enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	14:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	7	50	dep	%	1547:1547	arg1	20-30					1542:1546	20-30	1542:1546	20-30	1542:1546	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	9	51	theme	better	1929:1934	arg1	resolution					1941:1950	better peak resolution	1929:1950	better peak resolution	1929:1950	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	10	52	from	content	2116:2122	arg1	phase					2147:2151	the mobile phase	2136:2151	the mobile phase	2136:2151	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	7	53	theme	analytes	1571:1578	arg1	retention					1558:1566	the retention	1554:1566	the retention of analytes	1554:1578	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	1	54	from	HPLC	539:542	arg1	separation					430:439	the separation	426:439	the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC	426:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	10	55	theme	elution	2042:2048	arg1	reversal					2062:2069	enantiomer elution order (EEO) reversal	2031:2069	enantiomer elution order (EEO) reversal	2031:2069	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	0	56	with	drugs	42:46	arg1	columns					106:112	amylose- and cellulose- phenylcarbamate-based chiral columns	53:112	amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	53:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	6	57	dep	%	1353:1353	arg1	20-30					1348:1352	20-30	1348:1352	20-30	1348:1352	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	1	58	from	phases	529:534	arg1	HPLC					539:542	HPLC	539:542	HPLC	539:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	1	58	from	phases	529:534	arg1	acetonitrile					484:495	acetonitrile	484:495	acetonitrile	484:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	7	59	dep	20-30	1542:1546	arg1	to					1539:1540	to	1539:1540	to	1539:1540	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	10	60	theme	Interesting	2007:2017	arg1	examples					2019:2026	Interesting examples	2007:2026	Interesting examples of enantiomer elution order (EEO) reversal	2007:2069	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	10	61	theme	order	2050:2054	arg1	reversal					2062:2069	enantiomer elution order (EEO) reversal	2031:2069	enantiomer elution order (EEO) reversal	2031:2069	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	1	62	theme	amylose-	286:293	arg1	columns					338:344	amylose- and cellulose-phenylcarbamate-based chiral columns	286:344	amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries	286:392	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	3	63	theme	opposite	826:833	arg1	results					835:841	opposite results	826:841	opposite results for the studied set of chiral analytes	826:880	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	0	64	theme	electron-donor	225:238	arg1	effects					262:268	substituent electron-donor and electron-acceptor effects	213:268	substituent electron-donor and electron-acceptor effects	213:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	1	65	theme	aqueous-acetonitrile	501:520	arg1	phases					529:534	aqueous-acetonitrile mobile phases	501:534	aqueous-acetonitrile mobile phases in HPLC	501:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	1	66	theme	cellulose-phenylcarbamate-based	299:329	arg1	columns					338:344	amylose- and cellulose-phenylcarbamate-based chiral columns	286:344	amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries	286:392	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	8	67	theme	pure	1715:1718	arg1	acetonitrile					1720:1731	pure acetonitrile	1715:1731	pure acetonitrile	1715:1731	The important finding of this study is that the separation efficiency improves for most analytes when switching from pure acetonitrile to aqueous acetonitrile.					
32540066	6	68	theme	water	1305:1309	arg1	content					1311:1317	water content	1305:1317	water content in the mobile phase of up to 20-30% v/v	1305:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	9	69	theme	polar-organic	1979:1991	arg1	mode					2001:2004	pure polar-organic solvent mode	1974:2004	pure polar-organic solvent mode	1974:2004	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	3	70	contain	containing	665:674	arg2	substituents					723:734	only electron-donating or electron-withdrawing substituents	676:734	substituents	723:734	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	3	70	contain	containing	665:674	arg1	CSs					661:663	CSs	661:663	CSs containing only electron-donating or electron-withdrawing substituents	661:734	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	3	70	contain	containing	665:674	arg2	electron-donating					681:697	electron-donating	681:697	electron-donating	681:697	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	0	71	theme	electron-acceptor	244:260	arg1	effects					262:268	substituent electron-donor and electron-acceptor effects	213:268	substituent electron-donor and electron-acceptor effects	213:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	1	72	theme	phases	529:534	arg1	separation					430:439	the separation	426:439	the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC	426:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	4	73	theme	CS	960:961	arg1	chemistry					947:955	the chemistry	943:955	the chemistry of CS	943:961	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	7	74	theme	mobile	1523:1528	arg1	phase					1530:1534	the mobile phase up to 20-30% v/v	1519:1551	the mobile phase up to 20-30% v/v	1519:1551	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	10	75	theme	Lux	2176:2178	arg1	columns					2190:2196	Lux Amylose-2 columns	2176:2196	Lux Amylose-2 columns	2176:2196	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	3	76	theme	studied	851:857	arg1	set					859:861	the studied set	847:861	the studied set of chiral analytes	847:880	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	3	76	theme	studied	851:857	arg1	analytes					873:880	chiral analytes	866:880	chiral analytes	866:880	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	6	77	theme	v/v	1355:1357	arg1	phase					1333:1337	the mobile phase	1322:1337	the mobile phase of up to 20-30% v/v	1322:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	1	78	with	columns	338:344	arg1	chemistries					382:392	different chiral-selector (CS) chemistries	351:392	different chiral-selector (CS) chemistries	351:392	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	5	79	located	found	1126:1130	arg2	it					1119:1120	it	1119:1120	it	1119:1120	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	5	79	located	found	1126:1130	arg1	agreement					1087:1095	agreement	1087:1095	agreement with earlier results	1087:1116	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	9	80	theme	reduced	1781:1787	arg1	retention					1789:1797	reduced retention	1781:1797	reduced retention	1781:1797	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	7	81	theme	water	1502:1506	arg1	content					1508:1514	water content	1502:1514	water content in the mobile phase up to 20-30% v/v	1502:1551	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	3	82	theme	analytes	873:880	arg1	set					859:861	the studied set	847:861	the studied set of chiral analytes	847:880	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	3	82	theme	analytes	873:880	arg1	analytes					873:880	chiral analytes	866:880	chiral analytes	866:880	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	0	83	theme	high-performance	158:173	arg1	chromatography					182:195	high-performance liquid chromatography	158:195	high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects	158:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	6	84	from	phase	1333:1337	arg1	range					1296:1300	the range	1292:1300	the range of water content in the mobile phase of up to 20-30% v/v	1292:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	10	85	theme	mobile	2140:2145	arg1	phase					2147:2151	the mobile phase	2136:2151	the mobile phase	2136:2151	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	2	86	theme	immobilized	607:617	arg1	CSs					619:621	coated and immobilized CSs	596:621	coated and immobilized CSs	596:621	For two chemistries the amylose-based columns with coated and immobilized CSs were also compared.					
32540066	3	87	contain	containing	747:756	arg2	substituents					806:817	both electron-donating and electron-withdrawing substituents	758:817	substituents	806:817	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	3	87	contain	containing	747:756	arg1	those					741:745	those	741:745	those	741:745	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	6	88	theme	interaction	1411:1421	arg1	chromatography					1430:1443	hydrophilic interaction liquid chromatography	1399:1443	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	6	88	theme	interaction	1411:1421	arg1	HILIC					1446:1450	HILIC	1446:1450	HILIC	1446:1450	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	1	89	theme	basic	459:463	arg1	analytes					472:479	basic chiral analytes	459:479	basic chiral analytes in acetonitrile	459:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	4	90	theme	polysaccharide	996:1009	arg1	phenylcarbamates					1011:1026	polysaccharide phenylcarbamates	996:1026	polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents	996:1081	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	1	91	theme	enantiomers	444:454	arg1	separation					430:439	the separation	426:439	the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC	426:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	5	92	theme	accepted	1165:1172	arg1	view					1174:1177	the commonly accepted view	1152:1177	the commonly accepted view	1152:1177	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	3	93	theme	electron-withdrawing	702:721	arg1	substituents					723:734	only electron-donating or electron-withdrawing substituents	676:734	substituents	723:734	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	7	94	dep	phase	1530:1534	arg1	v/v					1549:1551	up to 20-30% v/v	1536:1551	the mobile phase up to 20-30% v/v	1519:1551	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	9	95	theme	enantiomers	1818:1828	arg1	separation					1804:1813	the separation	1800:1813	the separation of enantiomers	1800:1828	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	0	96	from	columns	106:112	arg1	aqueous-acetonitrile					134:153	aqueous-acetonitrile	134:153	aqueous-acetonitrile	134:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	0	96	from	columns	106:112	arg1	acetonitrile					117:128	acetonitrile	117:128	acetonitrile	117:128	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	6	97	theme	mobile	1326:1331	arg1	phase					1333:1337	the mobile phase	1322:1337	the mobile phase of up to 20-30% v/v	1322:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	3	98	theme	derivatives	919:929	arg1	case					889:892	the case	885:892	the case of amylose and cellulose derivatives	885:929	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	0	99	theme	phenylcarbamate-based	77:97	arg1	columns					106:112	amylose- and cellulose- phenylcarbamate-based chiral columns	53:112	amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	53:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	4	100	from	behavior	984:991	arg1	acetonitrile					1031:1042	acetonitrile	1031:1042	acetonitrile	1031:1042	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	4	100	from	behavior	984:991	arg1	acetonitrile					1059:1070	aqueous acetonitrile	1051:1070	aqueous acetonitrile	1051:1070	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	0	101	with	chromatography	182:195	arg1	focus					204:208	a focus	202:208	a focus on substituent electron-donor and electron-acceptor effects	202:268	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	2	102	theme	coated	596:601	arg1	CSs					619:621	coated and immobilized CSs	596:621	coated and immobilized CSs	596:621	For two chemistries the amylose-based columns with coated and immobilized CSs were also compared.					
32540066	8	103	theme	important	1602:1610	arg1	finding					1612:1618	The important finding	1598:1618	The important finding of this study	1598:1632	The important finding of this study is that the separation efficiency improves for most analytes when switching from pure acetonitrile to aqueous acetonitrile.					
32540066	6	104	theme	-type	1452:1456	arg1	adsorbents					1458:1467	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	9	105	theme	mobile	1868:1873	arg1	composition					1881:1891	mobile phase composition	1868:1891	mobile phase composition	1868:1891	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	6	106	from	content	1311:1317	arg1	phase					1333:1337	the mobile phase	1322:1337	the mobile phase of up to 20-30% v/v	1322:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	5	107	theme	reversed-phase	1237:1250	arg1	materials					1252:1260	typical reversed-phase materials	1229:1260	typical reversed-phase materials for basic analytes	1229:1279	In agreement with earlier results, it was found that in contrast to the commonly accepted view, polysaccharide phenylcarbamates do not behave as typical reversed-phase materials for basic analytes either.					
32540066	2	108	with	columns	583:589	arg1	CSs					619:621	coated and immobilized CSs	596:621	coated and immobilized CSs	596:621	For two chemistries the amylose-based columns with coated and immobilized CSs were also compared.					
32540066	6	109	theme	chromatography	1430:1443	arg1	adsorbents					1458:1467	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	hydrophilic interaction liquid chromatography (HILIC)-type adsorbents	1399:1467	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	8	110	theme	study	1628:1632	arg1	finding					1612:1618	The important finding	1598:1618	The important finding of this study	1598:1632	The important finding of this study is that the separation efficiency improves for most analytes when switching from pure acetonitrile to aqueous acetonitrile.					
32540066	4	111	from	acetonitrile	1059:1070	arg1	behavior					984:991	the behavior	980:991	the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents	980:1081	Along with the chemistry of CS the focus was on the behavior of polysaccharide phenylcarbamates in acetonitrile versus aqueous acetonitrile as eluents.					
32540066	1	112	theme	analytes	472:479	arg1	phases					529:534	aqueous-acetonitrile mobile phases	501:534	aqueous-acetonitrile mobile phases in HPLC	501:542	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	1	112	theme	analytes	472:479	arg1	enantiomers					444:454	enantiomers	444:454	enantiomers of basic chiral analytes in acetonitrile	444:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	0	113	theme	basic	36:40	arg1	drugs					42:46	chiral basic drugs	29:46	chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile	29:153	Separation of enantiomers of chiral basic drugs with amylose- and cellulose- phenylcarbamate-based chiral columns in acetonitrile and aqueous-acetonitrile in high-performance liquid chromatography with a focus on substituent electron-donor and electron-acceptor effects.					
32540066	1	114	theme	chiral-selector	361:375	arg1	chemistries					382:392	different chiral-selector (CS) chemistries	351:392	different chiral-selector (CS) chemistries	351:392	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
32540066	9	115	theme	analysis	1911:1918	arg1	time					1920:1923	shorter analysis time	1903:1923	shorter analysis time	1903:1923	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	8	116	theme	separation	1646:1655	arg1	efficiency					1657:1666	the separation efficiency	1642:1666	the separation efficiency	1642:1666	The important finding of this study is that the separation efficiency improves for most analytes when switching from pure acetonitrile to aqueous acetonitrile.					
32540066	6	117	from	similar	1388:1394	arg1	range					1296:1300	the range	1292:1300	the range of water content in the mobile phase of up to 20-30% v/v	1292:1357	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	3	118	theme	electron-withdrawing	785:804	arg1	substituents					806:817	both electron-donating and electron-withdrawing substituents	758:817	substituents	806:817	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	8	119	theme	aqueous	1736:1742	arg1	acetonitrile					1744:1755	aqueous acetonitrile	1736:1755	aqueous acetonitrile	1736:1755	The important finding of this study is that the separation efficiency improves for most analytes when switching from pure acetonitrile to aqueous acetonitrile.					
32540066	7	120	dep	v/v	1549:1551	arg1	%					1547:1547	%	1547:1547	%	1547:1547	This means that with increasing water content in the mobile phase up to 20-30% v/v, the retention of analytes mostly decreases.					
32540066	9	121	theme	peak	1936:1939	arg1	resolution					1941:1950	better peak resolution	1929:1950	better peak resolution	1929:1950	Therefore, in spite of reduced retention, the separation of enantiomers improves and thus, the HILIC-range of mobile phase composition, offering shorter analysis time and better peak resolution, is advantageous over pure polar-organic solvent mode.					
32540066	8	122	theme	most	1681:1684	arg1	analytes					1686:1693	most analytes	1681:1693	most analytes	1681:1693	The important finding of this study is that the separation efficiency improves for most analytes when switching from pure acetonitrile to aqueous acetonitrile.					
32540066	3	123	theme	electron-donating	763:779	arg1	substituents					806:817	both electron-donating and electron-withdrawing substituents	758:817	substituents	806:817	The comparison of CSs containing only electron-donating or electron-withdrawing substituents with those containing both electron-donating and electron-withdrawing substituents showed opposite results for the studied set of chiral analytes in the case of amylose and cellulose derivatives.					
32540066	6	124	dep	v/v	1355:1357	arg1	%					1353:1353	%	1353:1353	%	1353:1353	In the range of water content in the mobile phase of up to 20-30% v/v the behavior of these CSs is similar to hydrophilic interaction liquid chromatography (HILIC)-type adsorbents.					
32540066	10	125	theme	water	2127:2131	arg1	content					2116:2122	the content	2112:2122	the content of water in the mobile phase	2112:2151	Interesting examples of enantiomer elution order (EEO) reversal were observed for some analytes based on the content of water in the mobile phase on Lux Cellulose-1 and Lux Amylose-2 columns.					
32540066	1	126	from	analytes	472:479	arg1	acetonitrile					484:495	acetonitrile	484:495	acetonitrile	484:495	In this study, amylose- and cellulose-phenylcarbamate-based chiral columns with different chiral-selector (CS) chemistries were compared to each other for the separation of enantiomers of basic chiral analytes in acetonitrile and aqueous-acetonitrile mobile phases in HPLC.					
34623691	4	0	theme	cellulose	901:909	arg1	derivative					911:920	cellulose derivative	901:920	cellulose derivative	901:920	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	6	1	theme	column	1199:1204	arg1	temperature					1206:1216	column temperature	1199:1216	column temperature of 35°C	1199:1224	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	5	2	from	tetrahydrofuran	1036:1050	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of 500:500:20:2:1.5 (v/v/v/v/v)	1094:1134	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	3	3	dep	specific	705:712	arg1	method					694:699	the proposed method	681:699	the proposed method	681:699	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	3	dep	specific	705:712	arg1	stability					715:723	stability indicating	715:734	stability indicating	715:734	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	3	dep	specific	705:712	arg1	specific					705:712	specific	705:712	specific	705:712	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	4	4	theme	polar	873:877	arg1	solvents					887:894	polar organic solvents	873:894	polar organic solvents	873:894	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	6	5	theme	1.0 mL/min	1187:1196	arg1	volume					1237:1242	injection volume	1227:1242	injection volume of 10 μL	1227:1251	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	5	theme	1.0 mL/min	1187:1196	arg1	temperature					1206:1216	column temperature	1199:1216	column temperature of 35°C	1199:1224	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	5	theme	1.0 mL/min	1187:1196	arg1	rate					1179:1182	a flow rate	1172:1182	a flow rate of 1.0 mL/min	1172:1196	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	5	theme	1.0 mL/min	1187:1196	arg1	detection					1261:1269	UV detection	1258:1269	UV detection of 240 nm	1258:1279	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	6	theme	UV	1258:1259	arg1	detection					1261:1269	UV detection	1258:1269	UV detection of 240 nm	1258:1279	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	5	7	from	acetonitrile	1022:1033	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of 500:500:20:2:1.5 (v/v/v/v/v)	1094:1134	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	1	8	theme	simple	170:175	arg1	method					224:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	5	9	from	butylamine	1063:1072	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of 500:500:20:2:1.5 (v/v/v/v/v)	1094:1134	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	1	10	theme	sensitive	181:189	arg1	method					224:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	4	11	theme	stationary	951:960	arg1	phases					962:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	0	12	theme	tris	115:118	arg1	phase					161:165	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase	107:165	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	5	13	from	methanol	1053:1060	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of 500:500:20:2:1.5 (v/v/v/v/v)	1094:1134	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	1	14	theme	chiral	212:217	arg1	method					224:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	8	15	theme	Exdl	1409:1412	arg1	linearity					1443:1451	Exdl enantiomer detector response linearity	1409:1451	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL	1409:1501	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	6	16	theme	10 μL	1247:1251	arg1	volume					1237:1242	injection volume	1227:1242	injection volume of 10 μL	1227:1251	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	16	theme	10 μL	1247:1251	arg1	temperature					1206:1216	column temperature	1199:1216	column temperature of 35°C	1199:1224	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	16	theme	10 μL	1247:1251	arg1	rate					1179:1182	a flow rate	1172:1182	a flow rate of 1.0 mL/min	1172:1196	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	16	theme	10 μL	1247:1251	arg1	detection					1261:1269	UV detection	1258:1269	UV detection of 240 nm	1258:1279	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	0	17	theme	3,5-dichlorophenyl	120:137	arg1	phase					161:165	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase	107:165	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	4	18	theme	ether	929:933	arg1	phases					962:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	8	19	theme	detector	1425:1432	arg1	linearity					1443:1451	Exdl enantiomer detector response linearity	1409:1451	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL	1409:1501	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	4	20	theme	Amylose	738:744	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	3	21	theme	Exdl	620:623	arg1	enantiomer					625:634	Exdl enantiomer	620:634	Exdl enantiomer	620:634	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	7	22	theme	enantiomer	1342:1351	arg1	resolution					1319:1328	The United States Pharmacopeia (USP) resolution	1282:1328	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer	1282:1351	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	4	23	with	resolution	857:866	arg1	solvents					887:894	polar organic solvents	873:894	polar organic solvents	873:894	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	3	24	from	results	609:615	arg1	samples					652:658	all degraded samples	639:658	all degraded samples	639:658	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	1	25	theme	purity	359:364	arg1	determination					329:341	the determination	325:341	the determination of enantiomeric purity of eluxadoline (Exdl)	325:386	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	3	26	theme	degraded	643:650	arg1	samples					652:658	all degraded samples	639:658	all degraded samples	639:658	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	0	27	theme	purity	79:84	arg1	determination					49:61	the determination	45:61	the determination of enantiomeric purity of eluxadoline drug	45:104	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	1	28	theme	eluxadoline	369:379	arg1	purity					359:364	enantiomeric purity	346:364	enantiomeric purity of eluxadoline (Exdl)	346:386	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	8	29	theme	0.859-4.524 μg/mL	1485:1501	arg1	range					1476:1480	the concentration range	1458:1480	the concentration range of 0.859-4.524 μg/mL	1458:1501	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	4	30	theme	column	798:803	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	0	31	theme	eluxadoline	89:99	arg1	drug					101:104	eluxadoline drug	89:104	eluxadoline drug	89:104	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	2	32	theme	mobile	413:418	arg1	compositions					426:437	different mobile phase compositions	403:437	different mobile phase compositions	403:437	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	0	33	theme	enantiomeric	66:77	arg1	purity					79:84	enantiomeric purity	66:84	enantiomeric purity of eluxadoline drug	66:104	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	4	34	theme	carbamate	770:778	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	9	35	theme	detection	1545:1553	arg1	0.859 μg/mL					1653:1663	0.859 μg/mL	1653:1663	0.859 μg/mL	1653:1663	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	35	theme	detection	1545:1553	arg1	96.0					1670:1673	96.0	1670:1673	96.0	1670:1673	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	35	theme	detection	1545:1553	arg1	limit					1536:1540	The limit	1532:1540	The limit of detection, limit of quantification, and average percentage recovery values	1532:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	35	theme	detection	1545:1553	arg1	0.283 μg/mL					1640:1650	0.283 μg/mL	1640:1650	0.283 μg/mL	1640:1650	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	2	36	theme	phases	461:466	arg1	impact					393:398	The impact	389:398	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities	389:558	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	4	37	theme	stationary	781:790	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	2	38	theme	chiral	443:448	arg1	phases					461:466	chiral stationary phases	443:466	chiral stationary phases	443:466	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	0	39	theme	stability-indicating	8:27	arg1	method					34:39	A novel stability-indicating HPLC method	0:39	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	7	40	theme	65-min	1392:1397	arg1	time					1403:1406	a 65-min run time	1390:1406	a 65-min run time	1390:1406	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	9	41	theme	limit	1556:1560	arg1	0.859 μg/mL					1653:1663	0.859 μg/mL	1653:1663	0.859 μg/mL	1653:1663	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	41	theme	limit	1556:1560	arg1	96.0					1670:1673	96.0	1670:1673	96.0	1670:1673	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	41	theme	limit	1556:1560	arg1	limit					1536:1540	The limit	1532:1540	The limit of detection, limit of quantification, and average percentage recovery values	1532:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	41	theme	limit	1556:1560	arg1	0.283 μg/mL					1640:1650	0.283 μg/mL	1640:1650	0.283 μg/mL	1640:1650	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	2	42	theme	enantiomer	494:503	arg1	separation					475:484	the separation	471:484	the separation of Exdl enantiomer along with process- and degradation-related impurities	471:558	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	2	43	theme	compositions	426:437	arg1	impact					393:398	The impact	389:398	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities	389:558	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	9	44	theme	quantification	1565:1578	arg1	detection					1545:1553	detection	1545:1553	detection	1545:1553	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	44	theme	quantification	1565:1578	arg1	limit					1556:1560	limit	1556:1560	limit of quantification	1556:1578	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	44	theme	quantification	1565:1578	arg1	values					1613:1618	average percentage recovery values	1585:1618	average percentage recovery values	1585:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	7	45	theme	States	1293:1298	arg1	resolution					1319:1328	The United States Pharmacopeia (USP) resolution	1282:1328	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer	1282:1351	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	0	46	dep	method	34:39	arg1	phase					161:165	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase	107:165	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	9	47	theme	percentage	1593:1602	arg1	values					1613:1618	average percentage recovery values	1585:1618	average percentage recovery values	1585:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	6	48	theme	Isocratic	1137:1145	arg1	elution					1147:1153	Isocratic elution	1137:1153	Isocratic elution	1137:1153	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	9	49	theme	values	1613:1618	arg1	0.859 μg/mL					1653:1663	0.859 μg/mL	1653:1663	0.859 μg/mL	1653:1663	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	49	theme	values	1613:1618	arg1	96.0					1670:1673	96.0	1670:1673	96.0	1670:1673	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	49	theme	values	1613:1618	arg1	limit					1536:1540	The limit	1532:1540	The limit of detection, limit of quantification, and average percentage recovery values	1532:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	9	49	theme	values	1613:1618	arg1	0.283 μg/mL					1640:1650	0.283 μg/mL	1640:1650	0.283 μg/mL	1640:1650	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	6	50	theme	240 nm	1274:1279	arg1	volume					1237:1242	injection volume	1227:1242	injection volume of 10 μL	1227:1251	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	50	theme	240 nm	1274:1279	arg1	temperature					1206:1216	column temperature	1199:1216	column temperature of 35°C	1199:1224	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	50	theme	240 nm	1274:1279	arg1	rate					1179:1182	a flow rate	1172:1182	a flow rate of 1.0 mL/min	1172:1196	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	50	theme	240 nm	1274:1279	arg1	detection					1261:1269	UV detection	1258:1269	UV detection of 240 nm	1258:1279	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	1	51	theme	International	268:280	arg1	Conference					282:291	International Conference	268:291	International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl)	268:386	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	4	52	theme	derivative	911:920	arg1	phases					962:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	7	53	theme	run	1399:1401	arg1	time					1403:1406	a 65-min run time	1390:1406	a 65-min run time	1390:1406	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	3	54	theme	stable	602:607	arg1	results					609:615	stable results	602:615	stable results of Exdl enantiomer in all degraded samples	602:658	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	4	55	theme	organic	879:885	arg1	solvents					887:894	polar organic solvents	873:894	polar organic solvents	873:894	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	6	56	theme	flow	1174:1177	arg1	rate					1179:1182	a flow rate	1172:1182	a flow rate of 1.0 mL/min	1172:1196	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	3	57	theme	Exdl	593:596	arg1	Homogeneity					578:588	Homogeneity	578:588	Homogeneity of Exdl	578:596	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	57	theme	Exdl	593:596	arg1	results					609:615	stable results	602:615	stable results of Exdl enantiomer in all degraded samples	602:658	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	1	58	theme	stability-indicating	191:210	arg1	method					224:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	4	59	theme	zwitterion	940:949	arg1	phases					962:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	cellulose derivative, crown ether, and zwitterion stationary phases	901:967	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	8	60	theme	enantiomer	1414:1423	arg1	linearity					1443:1451	Exdl enantiomer detector response linearity	1409:1451	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL	1409:1501	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	0	61	theme	carbamate	139:147	arg1	phase					161:165	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase	107:165	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	3	62	theme	enantiomer	625:634	arg1	Homogeneity					578:588	Homogeneity	578:588	Homogeneity of Exdl	578:596	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	62	theme	enantiomer	625:634	arg1	results					609:615	stable results	602:615	stable results of Exdl enantiomer in all degraded samples	602:658	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	1	63	theme	HPLC	219:222	arg1	method					224:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method	168:229	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	8	64	theme	response	1434:1441	arg1	linearity					1443:1451	Exdl enantiomer detector response linearity	1409:1451	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL	1409:1501	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	0	65	theme	stationary	150:159	arg1	phase					161:165	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase	107:165	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	5	66	theme	500:500:20:2:1.5	1107:1122	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of 500:500:20:2:1.5 (v/v/v/v/v)	1094:1134	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	4	67	theme	crown	923:927	arg1	ether					929:933	crown ether	923:933	crown ether	923:933	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	6	68	theme	injection	1227:1235	arg1	volume					1237:1242	injection volume	1227:1242	injection volume of 10 μL	1227:1251	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	5	69	theme	acetic	1079:1084	arg1	acid					1086:1089	acetic acid	1079:1089	acetic acid	1079:1089	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	8	70	theme	concentration	1462:1474	arg1	range					1476:1480	the concentration range	1458:1480	the concentration range of 0.859-4.524 μg/mL	1458:1501	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	6	71	theme	35°C	1221:1224	arg1	volume					1237:1242	injection volume	1227:1242	injection volume of 10 μL	1227:1251	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	71	theme	35°C	1221:1224	arg1	temperature					1206:1216	column temperature	1199:1216	column temperature of 35°C	1199:1224	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	71	theme	35°C	1221:1224	arg1	rate					1179:1182	a flow rate	1172:1182	a flow rate of 1.0 mL/min	1172:1196	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	6	71	theme	35°C	1221:1224	arg1	detection					1261:1269	UV detection	1258:1269	UV detection of 240 nm	1258:1279	Isocratic elution was performed at a flow rate of 1.0 mL/min, column temperature of 35°C, injection volume of 10 μL, and UV detection of 240 nm.					
34623691	4	72	theme	tris	746:749	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	5	73	from	acid	1086:1089	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of 500:500:20:2:1.5 (v/v/v/v/v)	1094:1134	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	4	74	dep	IE-3	815:818	arg1	3 μm					835:838	3 μm	835:838	3 μm	835:838	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	4	74	dep	IE-3	815:818	arg1	150 × 4.6 mm					821:832	150 × 4.6 mm	821:832	150 × 4.6 mm	821:832	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	2	75	theme	process-	516:523	arg1	impurities					549:558	process- and degradation-related impurities	516:558	process- and degradation-related impurities	516:558	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	7	76	theme	Exdl	1337:1340	arg1	enantiomer					1342:1351	the Exdl enantiomer	1333:1351	the Exdl enantiomer	1333:1351	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	3	77	gly	Homogeneity	578:588	arg1	enantiomer					625:634	Exdl enantiomer	620:634	Exdl enantiomer	620:634	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	77	gly	Homogeneity	578:588	arg1	Exdl					593:596	Exdl	593:596	Exdl	593:596	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	1	78	from	Conference	282:291	arg1	guidelines					310:319	Harmonization guidelines	296:319	Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl)	296:386	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	3	79	from	Homogeneity	578:588	arg1	samples					652:658	all degraded samples	639:658	all degraded samples	639:658	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	0	80	theme	drug	101:104	arg1	purity					79:84	enantiomeric purity	66:84	enantiomeric purity of eluxadoline drug	66:104	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	5	81	theme	mobile	996:1001	arg1	phase					1003:1007	The mobile phase	992:1007	The mobile phase	992:1007	The mobile phase consisted of acetonitrile, tetrahydrofuran, methanol, butylamine, and acetic acid in the ratio of 500:500:20:2:1.5 (v/v/v/v/v).					
34623691	4	82	theme	nonpolar	973:980	arg1	solvents					982:989	nonpolar solvents	973:989	nonpolar solvents	973:989	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	4	83	theme	phase	792:796	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	0	84	theme	Amylose	107:113	arg1	phase					161:165	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase	107:165	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	2	85	theme	degradation-related	529:547	arg1	impurities					549:558	process- and degradation-related impurities	516:558	process- and degradation-related impurities	516:558	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	8	86	theme	R2	1519:1520	arg1	= 0.9985					1522:1529	R2 = 0.9985	1519:1529	R2 = 0.9985	1519:1529	Exdl enantiomer detector response linearity over the concentration range of 0.859-4.524 μg/mL was found to be R2 = 0.9985.					
34623691	4	87	theme	Chiralpak	805:813	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	2	88	theme	different	403:411	arg1	compositions					426:437	different mobile phase compositions	403:437	different mobile phase compositions	403:437	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	0	89	theme	novel	2:6	arg1	method					34:39	A novel stability-indicating HPLC method	0:39	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	7	90	theme	USP	1314:1316	arg1	resolution					1319:1328	The United States Pharmacopeia (USP) resolution	1282:1328	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer	1282:1351	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	4	91	theme	3,5-dichlorophenyl	751:768	arg1	IE-3					815:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3	738:818	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm)	738:839	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	0	92	theme	HPLC	29:32	arg1	method					34:39	A novel stability-indicating HPLC method	0:39	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.	0:166	A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.					
34623691	1	93	theme	Harmonization	296:308	arg1	guidelines					310:319	Harmonization guidelines	296:319	Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl)	296:386	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	7	94	theme	Pharmacopeia	1300:1311	arg1	resolution					1319:1328	The United States Pharmacopeia (USP) resolution	1282:1328	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer	1282:1351	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	2	95	theme	stationary	450:459	arg1	phases					461:466	chiral stationary phases	443:466	chiral stationary phases	443:466	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	7	96	theme	United	1286:1291	arg1	States					1293:1298	United States	1286:1298	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer	1282:1351	The United States Pharmacopeia (USP) resolution of the Exdl enantiomer was found to be more than 4.0 within a 65-min run time.					
34623691	2	97	theme	Exdl	489:492	arg1	enantiomer					494:503	Exdl enantiomer	489:503	Exdl enantiomer	489:503	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	2	98	theme	phase	420:424	arg1	compositions					426:437	different mobile phase compositions	403:437	different mobile phase compositions	403:437	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	2	99	from	impact	393:398	arg1	separation					475:484	the separation	471:484	the separation of Exdl enantiomer along with process- and degradation-related impurities	471:558	The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied.					
34623691	4	100	theme	better	850:855	arg1	resolution					857:866	better resolution	850:866	better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents	850:989	Amylose tris(3,5-dichlorophenyl carbamate) stationary phase column Chiralpak IE-3 (150 × 4.6 mm, 3 μm) provided better resolution with polar organic solvents than cellulose derivative, crown ether, and zwitterion stationary phases and nonpolar solvents.					
34623691	1	101	theme	enantiomeric	346:357	arg1	purity					359:364	enantiomeric purity	346:364	enantiomeric purity of eluxadoline (Exdl)	346:386	A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl).					
34623691	9	102	theme	average	1585:1591	arg1	values					1613:1618	average percentage recovery values	1585:1618	average percentage recovery values	1585:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	3	103	theme	proposed	685:692	arg1	method					694:699	the proposed method	681:699	the proposed method	681:699	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	103	theme	proposed	685:692	arg1	stability					715:723	stability indicating	715:734	stability indicating	715:734	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	103	theme	proposed	685:692	arg1	specific					705:712	specific	705:712	specific	705:712	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	9	104	theme	recovery	1604:1611	arg1	values					1613:1618	average percentage recovery values	1585:1618	average percentage recovery values	1585:1618	The limit of detection, limit of quantification, and average percentage recovery values were established as 0.283 μg/mL, 0.859 μg/mL, and 96.0, respectively.					
34623691	3	105	theme	indicating	725:734	arg1	method					694:699	the proposed method	681:699	the proposed method	681:699	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	105	theme	indicating	725:734	arg1	stability					715:723	stability indicating	715:734	stability indicating	715:734	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34623691	3	105	theme	indicating	725:734	arg1	specific					705:712	specific	705:712	specific	705:712	Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating).					
34507723	4	0	theme	intragastric	401:412	arg1	administration					414:427	intragastric administration	401:427	intragastric administration of 5000 international unit (IU) retinyl acetate	401:475	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	5	1	theme	UC	544:545	arg1	model					547:551	the UC model	540:551	the UC model mice to verify the effect of influenced gut microbiota	540:606	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	8	2	theme	abundant	867:874	arg1	diversity					890:898	much more abundant gut microbial diversity	857:898	much more abundant gut microbial diversity	857:898	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	11	3	theme	inflammation	1288:1299	arg1	barrier					1258:1264	intestinal barrier	1247:1264	intestinal barrier	1247:1264	The restore of intestinal barrier and the inhibition of inflammation were also found to contribute to the amelioration of UC by VA.					
34507723	11	3	theme	inflammation	1288:1299	arg1	inhibition					1274:1283	the inhibition	1270:1283	the inhibition of inflammation	1270:1299	The restore of intestinal barrier and the inhibition of inflammation were also found to contribute to the amelioration of UC by VA.					
34507723	5	4	used	used	526:529	arg2	FMT					512:514	FMT	512:514	FMT	512:514	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	5	4	used	used	526:529	arg2	transplantation					495:509	Fecal microbiota transplantation	478:509	Fecal microbiota transplantation (FMT)	478:515	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	9	5	theme	SCFAs	993:997	arg1	production					979:988	the increased production	965:988	the increased production of SCFAs in VA-treated mice	965:1016	Targeted metabolomics analysis manifested the increased production of SCFAs in VA-treated mice.					
34507723	12	6	theme	gut	1446:1448	arg1	microbiota					1450:1459	gut microbiota	1446:1459	gut microbiota	1446:1459	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	6	7	theme	cecal	659:663	arg1	contents					665:672	cecal contents	659:672	cecal contents	659:672	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	3	8	dep	%	336:336	arg1	w/v					339:341	w/v	339:341	w/v	339:341	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	8	9	theme	microbial	880:888	arg1	diversity					890:898	much more abundant gut microbial diversity	857:898	much more abundant gut microbial diversity	857:898	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	10	10	theme	gut	1147:1149	arg1	microbiota					1151:1160	gut microbiota	1147:1160	gut microbiota	1147:1160	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	10	11	theme	FMT	1048:1050	arg1	results					1052:1058	Gut microbiota depletion and FMT results	1019:1058	results	1052:1058	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	8	12	theme	flora	904:908	arg1	composition					910:920	flora composition	904:920	flora composition	904:920	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	10	13	from	increase	1163:1170	arg1	genera					1224:1229	UC-related genera	1213:1229	UC-related genera	1213:1229	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	10	13	from	increase	1163:1170	arg1	genera					1190:1195	SCFA-producing genera	1175:1195	SCFA-producing genera	1175:1195	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	7	14	theme	VA	747:748	arg1	supplementation					750:764	VA supplementation	747:764	VA supplementation	747:764	VA supplementation significantly ameliorated UC.					
34507723	9	15	theme	VA-treated	1002:1011	arg1	mice					1013:1016	VA-treated mice	1002:1016	VA-treated mice	1002:1016	Targeted metabolomics analysis manifested the increased production of SCFAs in VA-treated mice.					
34507723	12	16	from	amelioration	1465:1476	arg1	microbiota					1450:1459	gut microbiota	1446:1459	gut microbiota	1446:1459	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	9	17	from	production	979:988	arg1	mice					1013:1016	VA-treated mice	1002:1016	VA-treated mice	1002:1016	Targeted metabolomics analysis manifested the increased production of SCFAs in VA-treated mice.					
34507723	3	18	theme	UC	308:309	arg1	model					299:303	A mouse model	291:303	A mouse model of UC	291:309	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	5	19	theme	Fecal	478:482	arg1	transplantation					495:509	Fecal microbiota transplantation	478:509	Fecal microbiota transplantation (FMT)	478:515	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	5	19	theme	Fecal	478:482	arg1	FMT					512:514	FMT	512:514	FMT	512:514	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	1	20	theme	Ulcerative	93:102	arg1	condition					154:162	a chronic relapsing inflammatory condition	121:162	a chronic relapsing inflammatory condition of the gastrointestinal track	121:192	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	20	theme	Ulcerative	93:102	arg1	UC					113:114	UC	113:114	UC	113:114	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	20	theme	Ulcerative	93:102	arg1	colitis					104:110	Ulcerative colitis	93:110	Ulcerative colitis	93:110	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	21	theme	gastrointestinal	171:186	arg1	track					188:192	the gastrointestinal track	167:192	the gastrointestinal track	167:192	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	0	22	theme	A	8:8	arg1	supplementation					10:24	Vitamin A supplementation	0:24	Vitamin A supplementation	0:24	Vitamin A supplementation ameliorates ulcerative colitis in gut microbiota-dependent manner.					
34507723	10	23	theme	microbiota	1023:1032	arg1	depletion					1034:1042	Gut microbiota depletion and FMT results	1019:1058	depletion	1034:1042	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	5	24	theme	microbiota	484:493	arg1	transplantation					495:509	Fecal microbiota transplantation	478:509	Fecal microbiota transplantation (FMT)	478:515	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	5	24	theme	microbiota	484:493	arg1	FMT					512:514	FMT	512:514	FMT	512:514	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	10	25	from	decrease	1201:1208	arg1	genera					1224:1229	UC-related genera	1213:1229	UC-related genera	1213:1229	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	10	25	from	decrease	1201:1208	arg1	genera					1190:1195	SCFA-producing genera	1175:1195	SCFA-producing genera	1175:1195	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	1	26	theme	track	188:192	arg1	condition					154:162	a chronic relapsing inflammatory condition	121:162	a chronic relapsing inflammatory condition of the gastrointestinal track	121:192	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	26	theme	track	188:192	arg1	colitis					104:110	Ulcerative colitis	93:110	Ulcerative colitis	93:110	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	0	27	theme	Vitamin	0:6	arg1	supplementation					10:24	Vitamin A supplementation	0:24	Vitamin A supplementation	0:24	Vitamin A supplementation ameliorates ulcerative colitis in gut microbiota-dependent manner.					
34507723	4	28	theme	retinyl	461:467	arg1	acetate					469:475	5000 international unit (IU) retinyl acetate	432:475	5000 international unit (IU) retinyl acetate	432:475	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	11	29	theme	intestinal	1247:1256	arg1	barrier					1258:1264	intestinal barrier	1247:1264	intestinal barrier	1247:1264	The restore of intestinal barrier and the inhibition of inflammation were also found to contribute to the amelioration of UC by VA.					
34507723	8	30	theme	rRNA	800:803	arg1	sequencing					805:814	16S rRNA sequencing	796:814	16S rRNA sequencing	796:814	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	11	31	dep	The	1232:1234	arg1	restore					1236:1242	restore	1236:1242	restore	1236:1242	The restore of intestinal barrier and the inhibition of inflammation were also found to contribute to the amelioration of UC by VA.					
34507723	5	32	theme	influenced	582:591	arg1	microbiota					597:606	influenced gut microbiota	582:606	influenced gut microbiota	582:606	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	2	33	theme	study	215:219	arg1	purpose					199:205	The purpose	195:205	The purpose of this study	195:219	The purpose of this study is to explore whether Vitamin A (VA) can treat UC and its mechanisms.					
34507723	8	34	theme	16S	796:798	arg1	sequencing					805:814	16S rRNA sequencing	796:814	16S rRNA sequencing	796:814	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	3	35	theme	3.0	333:335	arg1	%					336:336	%	336:336	%	336:336	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	5	36	theme	gut	593:595	arg1	microbiota					597:606	influenced gut microbiota	582:606	influenced gut microbiota	582:606	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	12	37	theme	significant	1424:1434	arg1	change					1436:1441	a significant change	1422:1441	a significant change in gut microbiota	1422:1459	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	6	38	from	content	613:619	arg1	contents					665:672	cecal contents	659:672	cecal contents	659:672	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	3	39	theme	dextran	344:350	arg1	DSS					368:370	DSS	368:370	DSS	368:370	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	3	39	theme	dextran	344:350	arg1	sulfate					359:365	3.0% (w/v) dextran sodium sulfate	333:365	3.0% (w/v) dextran sodium sulfate (DSS)	333:371	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	4	40	theme	acetate	469:475	arg1	administration					414:427	intragastric administration	401:427	intragastric administration of 5000 international unit (IU) retinyl acetate	401:475	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	5	41	theme	microbiota	597:606	arg1	effect					572:577	the effect	568:577	the effect of influenced gut microbiota	568:606	Fecal microbiota transplantation (FMT) was also used to treat the UC model mice to verify the effect of influenced gut microbiota.					
34507723	0	42	theme	ulcerative	38:47	arg1	colitis					49:55	ulcerative colitis	38:55	ulcerative colitis	38:55	Vitamin A supplementation ameliorates ulcerative colitis in gut microbiota-dependent manner.					
34507723	4	43	theme	unit	451:454	arg1	acetate					469:475	5000 international unit (IU) retinyl acetate	432:475	5000 international unit (IU) retinyl acetate	432:475	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	3	44	theme	sodium	352:357	arg1	DSS					368:370	DSS	368:370	DSS	368:370	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	3	44	theme	sodium	352:357	arg1	sulfate					359:365	3.0% (w/v) dextran sodium sulfate	333:365	3.0% (w/v) dextran sodium sulfate (DSS)	333:371	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	8	45	theme	VA-treated	831:840	arg1	mice					842:845	VA-treated mice	831:845	VA-treated mice	831:845	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	11	46	theme	UC	1354:1355	arg1	amelioration					1338:1349	the amelioration	1334:1349	the amelioration of UC by VA	1334:1361	The restore of intestinal barrier and the inhibition of inflammation were also found to contribute to the amelioration of UC by VA.					
34507723	0	47	theme	gut	60:62	arg1	manner					85:90	gut microbiota-dependent manner	60:90	gut microbiota-dependent manner	60:90	Vitamin A supplementation ameliorates ulcerative colitis in gut microbiota-dependent manner.					
34507723	4	48	theme	international	437:449	arg1	acetate					469:475	5000 international unit (IU) retinyl acetate	432:475	5000 international unit (IU) retinyl acetate	432:475	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	0	49	theme	microbiota-dependent	64:83	arg1	manner					85:90	gut microbiota-dependent manner	60:90	gut microbiota-dependent manner	60:90	Vitamin A supplementation ameliorates ulcerative colitis in gut microbiota-dependent manner.					
34507723	9	50	theme	metabolomics	932:943	arg1	analysis					945:952	Targeted metabolomics analysis	923:952	Targeted metabolomics analysis	923:952	Targeted metabolomics analysis manifested the increased production of SCFAs in VA-treated mice.					
34507723	4	51	theme	IU	457:458	arg1	acetate					469:475	5000 international unit (IU) retinyl acetate	432:475	5000 international unit (IU) retinyl acetate	432:475	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	6	52	theme	mass	728:731	arg1	spectrometry					733:744	mass spectrometry	728:744	mass spectrometry	728:744	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	1	53	theme	inflammatory	141:152	arg1	condition					154:162	a chronic relapsing inflammatory condition	121:162	a chronic relapsing inflammatory condition of the gastrointestinal track	121:192	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	53	theme	inflammatory	141:152	arg1	colitis					104:110	Ulcerative colitis	93:110	Ulcerative colitis	93:110	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	6	54	theme	acids	642:646	arg1	content					613:619	The content	609:619	The content of short-chain fatty acids (SCFAs) in cecal contents	609:672	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	10	55	theme	microbiota-dependent	1078:1097	arg1	mechanism					1099:1107	the gut microbiota-dependent mechanism	1070:1107	the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota	1070:1160	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	10	56	dep	VA	1117:1118	arg1	relieved					1120:1127	relieved	1120:1127	VA relieved UC via regulating gut microbiota	1117:1160	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	6	57	theme	fatty	636:640	arg1	acids					642:646	short-chain fatty acids	624:646	short-chain fatty acids (SCFAs)	624:654	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	6	57	theme	fatty	636:640	arg1	SCFAs					649:653	SCFAs	649:653	SCFAs	649:653	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	12	58	theme	VA	1388:1389	arg1	supplement					1391:1400	a VA supplement	1386:1400	a VA supplement	1386:1400	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	10	59	theme	SCFA-producing	1175:1188	arg1	genera					1190:1195	SCFA-producing genera	1175:1195	SCFA-producing genera	1175:1195	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	12	60	theme	UC	1481:1482	arg1	amelioration					1465:1476	amelioration	1465:1476	amelioration of UC	1465:1482	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	12	60	theme	UC	1481:1482	arg1	change					1436:1441	a significant change	1422:1441	a significant change in gut microbiota	1422:1459	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	12	61	from	change	1436:1441	arg1	microbiota					1450:1459	gut microbiota	1446:1459	gut microbiota	1446:1459	It was concluded that a VA supplement was enough to cause a significant change in gut microbiota and amelioration of UC.					
34507723	6	62	theme	short-chain	624:634	arg1	acids					642:646	short-chain fatty acids	624:646	short-chain fatty acids (SCFAs)	624:654	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	6	62	theme	short-chain	624:634	arg1	SCFAs					649:653	SCFAs	649:653	SCFAs	649:653	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	1	63	theme	chronic	123:129	arg1	condition					154:162	a chronic relapsing inflammatory condition	121:162	a chronic relapsing inflammatory condition of the gastrointestinal track	121:192	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	63	theme	chronic	123:129	arg1	colitis					104:110	Ulcerative colitis	93:110	Ulcerative colitis	93:110	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	3	64	theme	mouse	293:297	arg1	model					299:303	A mouse model	291:303	A mouse model of UC	291:309	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	6	65	theme	gas	705:707	arg1	chromatography					709:722	gas chromatography	705:722	gas chromatography	705:722	The content of short-chain fatty acids (SCFAs) in cecal contents was quantitatively detected by gas chromatography and mass spectrometry.					
34507723	2	66	theme	Vitamin	243:249	arg1	VA					254:255	VA	254:255	VA	254:255	The purpose of this study is to explore whether Vitamin A (VA) can treat UC and its mechanisms.					
34507723	2	66	theme	Vitamin	243:249	arg1	A					251:251	Vitamin A	243:251	Vitamin A (VA)	243:256	The purpose of this study is to explore whether Vitamin A (VA) can treat UC and its mechanisms.					
34507723	1	67	theme	relapsing	131:139	arg1	condition					154:162	a chronic relapsing inflammatory condition	121:162	a chronic relapsing inflammatory condition of the gastrointestinal track	121:192	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	1	67	theme	relapsing	131:139	arg1	colitis					104:110	Ulcerative colitis	93:110	Ulcerative colitis	93:110	Ulcerative colitis (UC), is a chronic relapsing inflammatory condition of the gastrointestinal track.					
34507723	9	68	theme	increased	969:977	arg1	production					979:988	the increased production	965:988	the increased production of SCFAs in VA-treated mice	965:1016	Targeted metabolomics analysis manifested the increased production of SCFAs in VA-treated mice.					
34507723	9	69	theme	Targeted	923:930	arg1	analysis					945:952	Targeted metabolomics analysis	923:952	Targeted metabolomics analysis	923:952	Targeted metabolomics analysis manifested the increased production of SCFAs in VA-treated mice.					
34507723	4	70	used	used	381:384	arg2	VA					374:375	VA	374:375	VA	374:375	VA was used to treat UC by intragastric administration of 5000 international unit (IU) retinyl acetate.					
34507723	10	71	theme	Gut	1019:1021	arg1	depletion					1034:1042	Gut microbiota depletion and FMT results	1019:1058	depletion	1034:1042	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
34507723	8	72	theme	gut	876:878	arg1	diversity					890:898	much more abundant gut microbial diversity	857:898	much more abundant gut microbial diversity	857:898	16S rRNA sequencing indicated that VA-treated mice exhibited much more abundant gut microbial diversity and flora composition.					
34507723	3	73	theme	%	336:336	arg1	DSS					368:370	DSS	368:370	DSS	368:370	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	3	73	theme	%	336:336	arg1	sulfate					359:365	3.0% (w/v) dextran sodium sulfate	333:365	3.0% (w/v) dextran sodium sulfate (DSS)	333:371	A mouse model of UC was established using 3.0% (w/v) dextran sodium sulfate (DSS).					
34507723	10	74	theme	UC-related	1213:1222	arg1	genera					1224:1229	UC-related genera	1213:1229	UC-related genera	1213:1229	Gut microbiota depletion and FMT results confirmed the gut microbiota-dependent mechanism as that VA relieved UC via regulating gut microbiota: increase in SCFA-producing genera and decrease in UC-related genera.					
33248837	7	0	theme	resulting	829:837	arg1	pastes					839:844	the resulting pastes	825:844	the resulting pastes	825:844	In the resulting pastes, micrographs revealed that starch gelatinization depended on cocoyam variety.					
33248837	3	1	theme	chemical	430:437	arg1	composition					439:449	chemical composition	430:449	chemical composition	430:449	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	10	2	theme	Colocasia	1125:1133	arg1	spp					1135:1137	Colocasia spp	1125:1137	Colocasia spp	1125:1137	Lower protein digestibility was obtained in Colocasia spp.					
33248837	13	3	theme	cocoyams	1437:1444	arg1	applicability					1420:1432	the applicability	1416:1432	the applicability of cocoyams	1416:1444	The present findings offer ways to develop cocoyam based foods by using cormels, enhancing the applicability of cocoyams.					
33248837	9	4	theme	better	1027:1032	arg1	stability					1034:1042	better stability	1027:1042	better stability during storage with lower syneresis	1027:1078	flour led them to better stability during storage with lower syneresis.					
33248837	7	5	theme	cocoyam	907:913	arg1	variety					915:921	cocoyam variety	907:921	cocoyam variety	907:921	In the resulting pastes, micrographs revealed that starch gelatinization depended on cocoyam variety.					
33248837	0	6	from	Use	0:2	arg1	cormels					18:24	cormels	18:24	cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott	18:100	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	3	7	from	composition	439:449	arg1	sources					513:519	different botanical sources	493:519	different botanical sources	493:519	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	3	8	theme	cormels	480:486	arg1	composition					439:449	chemical composition	430:449	chemical composition	430:449	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	3	8	theme	cormels	480:486	arg1	properties					466:475	functional properties	455:475	functional properties	455:475	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	11	9	dep	index	1275:1279	arg1	65.84					1291:1295	65.84	1291:1295	65.84	1291:1295	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	11	9	dep	index	1275:1279	arg1	61.29					1282:1286	61.29	1282:1286	61.29	1282:1286	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	13	10	theme	cocoyam	1368:1374	arg1	foods					1382:1386	cocoyam based foods	1368:1386	cocoyam based foods	1368:1386	The present findings offer ways to develop cocoyam based foods by using cormels, enhancing the applicability of cocoyams.					
33248837	11	11	theme	in	1199:1200	arg1	hydrolysis					1215:1224	faster (higher k) in vitro starch hydrolysis	1181:1224	faster (higher k) in vitro starch hydrolysis	1181:1224	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	5	12	theme	gel	763:765	arg1	strength					767:774	Amylab gel strength	756:774	Amylab gel strength	756:774	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	11	13	theme	starch	1208:1213	arg1	hydrolysis					1215:1224	faster (higher k) in vitro starch hydrolysis	1181:1224	faster (higher k) in vitro starch hydrolysis	1181:1224	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	11	14	dep	showed	1174:1179	arg1	0.0050					1237:1242	0.0050	1237:1242	0.0050	1237:1242	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	11	14	dep	showed	1174:1179	arg1	0.0140					1227:1232	0.0140	1227:1232	0.0140	1227:1232	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	10	15	theme	protein	1087:1093	arg1	digestibility					1095:1107	Lower protein digestibility	1081:1107	Lower protein digestibility	1081:1107	Lower protein digestibility was obtained in Colocasia spp.					
33248837	2	16	theme	secondary	349:357	arg1	corms					359:363	the secondary corms	345:363	the secondary corms	345:363	Nevertheless, the secondary corms, also named cormels, are not fully exploited.					
33248837	11	17	theme	faster	1181:1186	arg1	hydrolysis					1215:1224	faster (higher k) in vitro starch hydrolysis	1181:1224	faster (higher k) in vitro starch hydrolysis	1181:1224	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	1	18	dep	Xanthosoma	262:271	arg1	sagittifolium					273:285	Xanthosoma sagittifolium	262:285	Xanthosoma sagittifolium	262:285	The corms of cocoyams, specifically Colocasia esculenta (L.) Schott and Xanthosoma sagittifolium (L.) Schott are usually consumed as pastes.					
33248837	0	19	theme	nutritional	160:170	arg1	characterization					172:187	nutritional characterization	160:187	nutritional characterization	160:187	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	11	20	dep	gels	1140:1143	arg1	%					1151:1151	67.56%	1146:1151	67.56%	1146:1151	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	11	20	dep	gels	1140:1143	arg1	%					1161:1161	70.91%	1156:1161	70.91%	1156:1161	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	13	21	theme	based	1376:1380	arg1	foods					1382:1386	cocoyam based foods	1368:1386	cocoyam based foods	1368:1386	The present findings offer ways to develop cocoyam based foods by using cormels, enhancing the applicability of cocoyams.					
33248837	5	22	theme	oxalates	701:708	arg1	content					727:733	ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content	676:733	content	727:733	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	0	23	from	cormels	18:24	arg1	flour					7:11	flour	7:11	flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods	7:124	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	0	23	from	cormels	18:24	arg1	Use					0:2	Use	0:2	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.	0:188	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	9	24	theme	lower	1064:1068	arg1	syneresis					1070:1078	lower syneresis	1064:1078	lower syneresis	1064:1078	flour led them to better stability during storage with lower syneresis.					
33248837	5	25	dep	ash	676:678	arg1	%					685:685	5.65%	681:685	5.65%	681:685	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	25	dep	ash	676:678	arg1	%					694:694	5.05%	690:694	5.05%	690:694	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	26	dep	contained	618:626	arg1	%					663:663	10.32%	658:663	10.32%	658:663	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	26	dep	contained	618:626	arg1	%					672:672	9.65%	668:672	9.65%	668:672	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	8	27	theme	closed	951:956	arg1	microstructure					958:971	the very tight and closed microstructure	932:971	the very tight and closed microstructure of pastes	932:981	Indeed, the very tight and closed microstructure of pastes containing Colocasia spp.					
33248837	3	28	dep	composition	439:449	arg1	the					426:428	the	426:428	the	426:428	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	11	29	theme	glycemic	1266:1273	arg1	index					1275:1279	estimated glycemic index	1256:1279	lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp	1250:1316	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	3	30	theme	different	493:501	arg1	sources					513:519	different botanical sources	493:519	different botanical sources	493:519	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	0	31	theme	flour	7:11	arg1	Use					0:2	Use	0:2	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.	0:188	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	0	32	theme	L.	91:92	arg1	cormels					18:24	cormels	18:24	cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott	18:100	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	3	33	theme	botanical	503:511	arg1	sources					513:519	different botanical sources	493:519	different botanical sources	493:519	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	11	34	dep	in	1199:1200	arg1	vitro					1202:1206	vitro	1202:1206	vitro	1202:1206	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	13	35	theme	present	1329:1335	arg1	findings					1337:1344	The present findings	1325:1344	The present findings	1325:1344	The present findings offer ways to develop cocoyam based foods by using cormels, enhancing the applicability of cocoyams.					
33248837	11	36	theme	estimated	1256:1264	arg1	index					1275:1279	estimated glycemic index	1256:1279	lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp	1250:1316	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	11	37	theme	higher	1189:1194	arg1	k					1196:1196	higher k	1189:1196	higher k	1189:1196	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	8	38	theme	tight	941:945	arg1	microstructure					958:971	the very tight and closed microstructure	932:971	the very tight and closed microstructure of pastes	932:981	Indeed, the very tight and closed microstructure of pastes containing Colocasia spp.					
33248837	3	39	theme	pastes	576:581	arg1	digestibility					545:557	the digestibility	541:557	the digestibility of the resulting pastes	541:581	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	0	40	dep	Xanthosoma	29:38	arg1	Schott					95:100	Schott	95:100	Schott	95:100	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	0	40	dep	Xanthosoma	29:38	arg1	Schott					59:64	Schott	59:64	Schott	59:64	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	0	40	dep	Xanthosoma	29:38	arg1	sagittifolium					40:52	Xanthosoma sagittifolium (L.)	29:57	Xanthosoma sagittifolium (L.)	29:57	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	9	41	with	storage	1051:1057	arg1	syneresis					1070:1078	lower syneresis	1064:1078	lower syneresis	1064:1078	flour led them to better stability during storage with lower syneresis.					
33248837	0	42	theme	pastes	113:118	arg1	foods					120:124	pastes foods	113:124	pastes foods	113:124	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	5	43	dep	content	727:733	arg1	%					724:724	0.22%	720:724	0.22%	720:724	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	43	dep	content	727:733	arg1	%					715:715	0.32%	711:715	0.32%	711:715	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	7	44	theme	starch	873:878	arg1	gelatinization					880:893	starch gelatinization	873:893	starch gelatinization	873:893	In the resulting pastes, micrographs revealed that starch gelatinization depended on cocoyam variety.					
33248837	0	45	theme	Xanthosoma	29:38	arg1	cormels					18:24	cormels	18:24	cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott	18:100	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	5	46	theme	higher	642:647	arg1	protein					649:655	significantly higher protein	628:655	significantly higher protein	628:655	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	11	47	dep	faster	1181:1186	arg1	k					1196:1196	higher k	1189:1196	higher k	1189:1196	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	8	48	theme	pastes	976:981	arg1	microstructure					958:971	the very tight and closed microstructure	932:971	the very tight and closed microstructure of pastes	932:981	Indeed, the very tight and closed microstructure of pastes containing Colocasia spp.					
33248837	5	49	theme	Xanthosoma	799:808	arg1	spp					810:812	Xanthosoma spp	799:812	Xanthosoma spp	799:812	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	50	contain	contained	618:626	arg2	ash					676:678	ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content	676:733	ash	676:678	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	50	contain	contained	618:626	arg1	flour					612:616	flour	612:616	flour	612:616	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	50	contain	contained	618:626	arg2	content					727:733	ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content	676:733	content	727:733	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	50	contain	contained	618:626	arg2	protein					649:655	significantly higher protein	628:655	significantly higher protein	628:655	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	3	51	theme	resulting	566:574	arg1	pastes					576:581	the resulting pastes	562:581	the resulting pastes	562:581	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	11	52	dep	lower	1250:1254	arg1	index					1275:1279	estimated glycemic index	1256:1279	lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp	1250:1316	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	5	53	theme	Amylab	756:761	arg1	strength					767:774	Amylab gel strength	756:774	Amylab gel strength	756:774	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	3	54	from	sources	513:519	arg1	cormels					480:486	cormels	480:486	cormels from different botanical sources	480:519	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	3	54	from	sources	513:519	arg1	composition					439:449	chemical composition	430:449	chemical composition	430:449	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	3	54	from	sources	513:519	arg1	properties					466:475	functional properties	455:475	functional properties	455:475	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	8	55	theme	Colocasia	994:1002	arg1	spp					1004:1006	Colocasia spp	994:1006	Colocasia spp	994:1006	Indeed, the very tight and closed microstructure of pastes containing Colocasia spp.					
33248837	5	56	dep	lower	750:754	arg1	773 g					777:781	773 g	777:781	773 g	777:781	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	56	dep	lower	750:754	arg1	strength					767:774	Amylab gel strength	756:774	Amylab gel strength	756:774	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	5	56	dep	lower	750:754	arg1	1040 g					786:791	1040 g	786:791	1040 g	786:791	flour contained significantly higher protein (10.32% vs 9.65%), ash (5.65% vs 5.05%) and oxalates (0.32% vs 0.22%) content, and exhibited lower Amylab gel strength (773 g vs 1040 g) than Xanthosoma spp.					
33248837	11	57	theme	Xanthosoma	1303:1312	arg1	spp					1314:1316	Xanthosoma spp	1303:1316	Xanthosoma spp	1303:1316	gels (67.56% vs 70.91%), but they showed faster (higher k) in vitro starch hydrolysis (0.0140 vs 0.0050) with lower estimated glycemic index (61.29 vs 65.84) than Xanthosoma spp.					
33248837	10	58	theme	Lower	1081:1085	arg1	digestibility					1095:1107	Lower protein digestibility	1081:1107	Lower protein digestibility	1081:1107	Lower protein digestibility was obtained in Colocasia spp.					
33248837	1	59	theme	cocoyams	203:210	arg1	corms					194:198	The corms	190:198	The corms of cocoyams, specifically Colocasia esculenta (L.) Schott and Xanthosoma sagittifolium (L.) Schott	190:297	The corms of cocoyams, specifically Colocasia esculenta (L.) Schott and Xanthosoma sagittifolium (L.) Schott are usually consumed as pastes.					
33248837	1	59	theme	cocoyams	203:210	arg1	pastes					323:328	pastes	323:328	pastes	323:328	The corms of cocoyams, specifically Colocasia esculenta (L.) Schott and Xanthosoma sagittifolium (L.) Schott are usually consumed as pastes.					
33248837	0	60	dep	Use	0:2	arg1	functional					145:154	functional	145:154	functional	145:154	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	0	60	dep	Use	0:2	arg1	characterization					172:187	nutritional characterization	160:187	nutritional characterization	160:187	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	0	60	dep	Use	0:2	arg1	Physico-chemical					127:142	Physico-chemical	127:142	Physico-chemical	127:142	Use of flour from cormels of Xanthosoma sagittifolium (L.) Schott and Colocasia esculenta (L.) Schott to develop pastes foods: Physico-chemical, functional and nutritional characterization.					
33248837	3	61	theme	functional	455:464	arg1	properties					466:475	functional properties	455:475	functional properties	455:475	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33248837	3	62	from	properties	466:475	arg1	sources					513:519	different botanical sources	493:519	different botanical sources	493:519	In this study, the chemical composition and functional properties of cormels from different botanical sources were evaluated, and the digestibility of the resulting pastes investigated.					
33567351	3	0	theme	medium	865:870	arg1	chains					886:891	medium B-type glucan chains	865:891	medium B-type glucan chains	865:891	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	1	1	theme	semolina	344:351	arg1	one-quarter					317:327	one-quarter	317:327	one-quarter of durum wheat semolina	317:351	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	2	2	theme	protein	496:502	arg1	concentrate					504:514	protein concentrate	496:514	protein concentrate	496:514	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	3	3	theme	C-type	902:907	arg1	chains					916:921	long C-type glucan chains	897:921	long C-type glucan chains	897:921	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	2	4	theme	semolina	708:715	arg1	control					717:723	durum wheat semolina control	696:723	durum wheat semolina control	696:723	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	1	5	theme	nutritional	367:377	arg1	quality					379:385	nutritional quality	367:385	nutritional quality	367:385	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	6	theme	bean	164:167	arg1	flour					185:189	Faba bean (Vicia faba L.) flour	159:189	Faba bean (Vicia faba L.) flour	159:189	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	2	7	theme	dietary	636:642	arg1	fiber					644:648	increased total dietary fiber	620:648	increased total dietary fiber	620:648	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	7	theme	dietary	636:642	arg1	p ≤ 0.001					680:688	p ≤ 0.001	680:688	p ≤ 0.001	680:688	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	1	8	theme	Vicia	170:174	arg1	L.					181:182	Vicia faba L.	170:182	Vicia faba L.	170:182	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	8	theme	Vicia	170:174	arg1	bean					164:167	Faba bean	159:167	Faba bean (Vicia faba L.) flour	159:189	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	9	theme	chains	916:921	arg1	proportion					851:860	increased proportion	841:860	increased proportion of medium B-type glucan chains and long C-type glucan chains	841:921	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	5	10	from	contributions	1167:1179	arg1	outcomes					1217:1224	human health outcomes	1204:1224	human health outcomes	1204:1224	The results suggest differential contributions of food ingredients in human health outcomes.					
33567351	2	11	theme	starch	586:591	arg1	concentrations					593:606	lower (p ≤ 0.001) total starch concentrations	562:606	lower (p ≤ 0.001) total starch concentrations	562:606	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	12	theme	digestible	661:670	arg1	starch					672:677	slowly digestible starch	654:677	slowly digestible starch	654:677	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	1	13	dep	starch	192:197	arg1	concentrate					199:209	concentrate	199:209	concentrate	199:209	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	4	14	theme	increased	1057:1065	arg1	protein					1067:1073	increased protein	1057:1073	increased protein	1057:1073	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	0	15	theme	in	135:136	arg1	digestibility					144:156	in vitro digestibility	135:156	in vitro digestibility	135:156	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	2	16	theme	pasta	468:472	arg1	samples					474:480	The raw pasta samples	460:480	The raw pasta samples prepared with protein concentrate or isolate	460:525	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	17	theme	total	580:584	arg1	concentrations					593:606	lower (p ≤ 0.001) total starch concentrations	562:606	lower (p ≤ 0.001) total starch concentrations	562:606	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	1	18	theme	durum	332:336	arg1	semolina					344:351	durum wheat semolina	332:351	durum wheat semolina	332:351	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	0	19	dep	in	135:136	arg1	vitro					138:142	vitro	138:142	vitro	138:142	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	1	20	dep	%	214:214	arg1	starch					216:221	starch	216:221	60% starch	212:221	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	4	21	theme	bean	1032:1035	arg1	pasta					1047:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	5	22	theme	ingredients	1189:1199	arg1	contributions					1167:1179	differential contributions	1154:1179	differential contributions of food ingredients in human health outcomes	1154:1224	The results suggest differential contributions of food ingredients in human health outcomes.					
33567351	2	23	contain	had	527:529	arg2	p ≤ 0.001					680:688	p ≤ 0.001	680:688	p ≤ 0.001	680:688	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	23	contain	had	527:529	arg2	concentrations					593:606	lower (p ≤ 0.001) total starch concentrations	562:606	lower (p ≤ 0.001) total starch concentrations	562:606	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	23	contain	had	527:529	arg1	samples					474:480	The raw pasta samples	460:480	The raw pasta samples prepared with protein concentrate or isolate	460:525	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	23	contain	had	527:529	arg2	fiber					644:648	increased total dietary fiber	620:648	increased total dietary fiber	620:648	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	23	contain	had	527:529	arg2	protein					550:556	higher (p ≤ 0.001) protein	531:556	higher (p ≤ 0.001) protein	531:556	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	0	24	theme	wheat	57:61	arg1	pasta					63:67	durum wheat pasta	51:67	durum wheat pasta	51:67	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	5	25	theme	differential	1154:1165	arg1	contributions					1167:1179	differential contributions	1154:1179	differential contributions of food ingredients in human health outcomes	1154:1224	The results suggest differential contributions of food ingredients in human health outcomes.					
33567351	2	26	theme	total	630:634	arg1	fiber					644:648	increased total dietary fiber	620:648	increased total dietary fiber	620:648	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	26	theme	total	630:634	arg1	p ≤ 0.001					680:688	p ≤ 0.001	680:688	p ≤ 0.001	680:688	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	1	27	theme	~85	281:283	arg1	%					284:284	%	284:284	%	284:284	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	28	theme	faba	791:794	arg1	pasta					811:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	1	29	theme	pasta	453:457	arg1	quality					379:385	nutritional quality	367:385	nutritional quality	367:385	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	29	theme	pasta	453:457	arg1	functions					405:413	physiological functions	391:413	physiological functions	391:413	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	0	30	theme	noodle	94:99	arg1	composition					101:111	noodle composition	94:111	noodle composition	94:111	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	3	31	theme	related	988:994	arg1	effects					996:1002	glycaemia related effects	978:1002	glycaemia related effects in the human diet	978:1020	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	0	32	theme	Faba	0:3	arg1	meal					10:13	Faba bean meal	0:13	Faba bean meal	0:13	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	2	33	theme	lower	562:566	arg1	concentrations					593:606	lower (p ≤ 0.001) total starch concentrations	562:606	lower (p ≤ 0.001) total starch concentrations	562:606	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	5	34	theme	human	1204:1208	arg1	outcomes					1217:1224	human health outcomes	1204:1224	human health outcomes	1204:1224	The results suggest differential contributions of food ingredients in human health outcomes.					
33567351	2	35	theme	increased	620:628	arg1	fiber					644:648	increased total dietary fiber	620:648	increased total dietary fiber	620:648	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	35	theme	increased	620:628	arg1	p ≤ 0.001					680:688	p ≤ 0.001	680:688	p ≤ 0.001	680:688	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	3	36	theme	human	1011:1015	arg1	diet					1017:1020	the human diet	1007:1020	the human diet	1007:1020	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	3	37	theme	fortified	801:809	arg1	pasta					811:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	1	38	theme	~60	246:248	arg1	%					249:249	%	249:249	%	249:249	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	39	theme	increased	841:849	arg1	proportion					851:860	increased proportion	841:860	increased proportion of medium B-type glucan chains and long C-type glucan chains	841:921	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	0	40	theme	protein	26:32	arg1	fortification					34:46	protein fortification	26:46	protein fortification	26:46	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	2	41	theme	wheat	702:706	arg1	control					717:723	durum wheat semolina control	696:723	durum wheat semolina control	696:723	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	1	42	theme	wheat	424:428	arg1	pasta					453:457	durum wheat (Triticum turgidum L.) pasta	418:457	durum wheat (Triticum turgidum L.) pasta	418:457	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	43	theme	B-type	872:877	arg1	chains					886:891	medium B-type glucan chains	865:891	medium B-type glucan chains	865:891	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	2	44	theme	whole	745:749	arg1	flour					761:765	added whole faba-bean flour	739:765	added whole faba-bean flour	739:765	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	4	45	contain	had	1053:1055	arg1	pasta					1047:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	4	45	contain	had	1053:1055	arg2	fiber					1087:1091	dietary fiber	1079:1091	dietary fiber	1079:1091	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	4	45	contain	had	1053:1055	arg2	protein					1067:1073	increased protein	1057:1073	increased protein	1057:1073	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	3	46	theme	long	897:900	arg1	chains					916:921	long C-type glucan chains	897:921	long C-type glucan chains	897:921	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	2	47	theme	isolated	770:777	arg1	starch					779:784	isolated starch	770:784	isolated starch	770:784	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	3	48	theme	chains	886:891	arg1	proportion					851:860	increased proportion	841:860	increased proportion of medium B-type glucan chains and long C-type glucan chains	841:921	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	1	49	theme	Faba	159:162	arg1	L.					181:182	Vicia faba L.	170:182	Vicia faba L.	170:182	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	49	theme	Faba	159:162	arg1	bean					164:167	Faba bean	159:167	Faba bean (Vicia faba L.) flour	159:189	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	2	50	theme	higher	531:536	arg1	protein					550:556	higher (p ≤ 0.001) protein	531:556	higher (p ≤ 0.001) protein	531:556	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	4	51	theme	faba	1027:1030	arg1	pasta					1047:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	3	52	theme	glucan	909:914	arg1	chains					916:921	long C-type glucan chains	897:921	long C-type glucan chains	897:921	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	0	53	theme	starch	114:119	arg1	structure					121:129	starch structure	114:129	starch structure	114:129	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	1	54	theme	physiological	391:403	arg1	functions					405:413	physiological functions	391:413	physiological functions	391:413	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	55	theme	faba	176:179	arg1	L.					181:182	Vicia faba L.	170:182	Vicia faba L.	170:182	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	55	theme	faba	176:179	arg1	bean					164:167	Faba bean	159:167	Faba bean (Vicia faba L.) flour	159:189	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	2	56	dep	higher	531:536	arg1	p ≤ 0.001					539:547	p ≤ 0.001	539:547	p ≤ 0.001	539:547	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	3	57	contain	had	817:819	arg1	pasta					811:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	3	57	contain	had	817:819	arg2	starch					829:834	altered starch	821:834	altered starch	821:834	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	4	58	theme	dietary	1079:1085	arg1	fiber					1087:1091	dietary fiber	1079:1091	dietary fiber	1079:1091	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	2	59	dep	lower	562:566	arg1	p ≤ 0.001					569:577	p ≤ 0.001	569:577	p ≤ 0.001	569:577	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	60	theme	raw	464:466	arg1	samples					474:480	The raw pasta samples	460:480	The raw pasta samples prepared with protein concentrate or isolate	460:525	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	4	61	theme	fortified	1037:1045	arg1	pasta					1047:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta	1023:1051	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	5	62	theme	food	1184:1187	arg1	ingredients					1189:1199	food ingredients	1184:1199	food ingredients	1184:1199	The results suggest differential contributions of food ingredients in human health outcomes.					
33567351	0	63	theme	durum	51:55	arg1	pasta					63:67	durum wheat pasta	51:67	durum wheat pasta	51:67	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	1	64	theme	wheat	338:342	arg1	semolina					344:351	durum wheat semolina	332:351	durum wheat semolina	332:351	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	0	65	theme	pasta	63:67	arg1	meal					10:13	Faba bean meal	0:13	Faba bean meal	0:13	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	0	65	theme	pasta	63:67	arg1	starch					16:21	starch	16:21	starch	16:21	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	0	65	theme	pasta	63:67	arg1	fortification					34:46	protein fortification	26:46	protein fortification	26:46	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	1	66	theme	protein	264:270	arg1	isolate					272:278	protein isolate	264:278	protein isolate (~85% protein)	264:293	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	66	theme	protein	264:270	arg1	protein					286:292	~85% protein	281:292	~85% protein	281:292	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	67	from	effects	996:1002	arg1	diet					1017:1020	the human diet	1007:1020	the human diet	1007:1020	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	3	68	theme	starch	932:937	arg1	digestibility					939:951	starch digestibility	932:951	starch digestibility	932:951	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	1	69	dep	quality	379:385	arg1	the					363:365	the	363:365	the	363:365	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	70	theme	%	284:284	arg1	isolate					272:278	protein isolate	264:278	protein isolate (~85% protein)	264:293	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	70	theme	%	284:284	arg1	protein					286:292	~85% protein	281:292	~85% protein	281:292	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	71	theme	glycaemia	978:986	arg1	effects					996:1002	glycaemia related effects	978:1002	glycaemia related effects in the human diet	978:1020	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	2	72	dep	along	609:613	arg1	with					615:618	with	615:618	with	615:618	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	0	73	theme	bean	5:8	arg1	meal					10:13	Faba bean meal	0:13	Faba bean meal	0:13	Faba bean meal, starch or protein fortification of durum wheat pasta differentially influence noodle composition, starch structure and in vitro digestibility.					
33567351	4	74	theme	food	1109:1112	arg1	intake					1114:1119	food intake	1109:1119	food intake	1109:1119	The faba bean fortified pasta had increased protein and dietary fiber that influenced food intake and satiety.					
33567351	1	75	theme	protein	225:231	arg1	concentrate					233:243	protein concentrate	225:243	protein concentrate (~60% protein)	225:258	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	75	theme	protein	225:231	arg1	protein					251:257	~60% protein	246:257	~60% protein	246:257	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	3	76	theme	altered	821:827	arg1	starch					829:834	altered starch	821:834	altered starch	821:834	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	5	77	theme	health	1210:1215	arg1	outcomes					1217:1224	human health outcomes	1204:1224	human health outcomes	1204:1224	The results suggest differential contributions of food ingredients in human health outcomes.					
33567351	3	78	theme	bean	796:799	arg1	pasta					811:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta	787:815	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	1	79	dep	Triticum	431:438	arg1	L.					449:450	Triticum turgidum L.	431:450	Triticum turgidum L.	431:450	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	80	theme	%	249:249	arg1	concentrate					233:243	protein concentrate	225:243	protein concentrate (~60% protein)	225:258	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	80	theme	%	249:249	arg1	protein					251:257	~60% protein	246:257	~60% protein	246:257	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	81	theme	durum	418:422	arg1	Triticum					431:438	Triticum	431:438	Triticum	431:438	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	1	81	theme	durum	418:422	arg1	wheat					424:428	durum wheat	418:428	durum wheat (Triticum turgidum L.) pasta	418:457	Faba bean (Vicia faba L.) flour, starch concentrate (60% starch), protein concentrate (~60% protein) and protein isolate (~85% protein) were added to replace one-quarter of durum wheat semolina to enrich the nutritional quality and physiological functions of durum wheat (Triticum turgidum L.) pasta.					
33567351	2	82	theme	durum	696:700	arg1	control					717:723	durum wheat semolina control	696:723	durum wheat semolina control	696:723	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	2	83	theme	faba-bean	751:759	arg1	flour					761:765	added whole faba-bean flour	739:765	added whole faba-bean flour	739:765	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33567351	3	84	theme	glucan	879:884	arg1	chains					886:891	medium B-type glucan chains	865:891	medium B-type glucan chains	865:891	The faba bean fortified pasta had altered starch with increased proportion of medium B-type glucan chains and long C-type glucan chains, reduced starch digestibility and were associated with glycaemia related effects in the human diet.					
33567351	2	85	theme	added	739:743	arg1	flour					761:765	added whole faba-bean flour	739:765	added whole faba-bean flour	739:765	The raw pasta samples prepared with protein concentrate or isolate had higher (p ≤ 0.001) protein and lower (p ≤ 0.001) total starch concentrations, along with increased total dietary fiber and slowly digestible starch (p ≤ 0.001) than durum wheat semolina control or those with added whole faba-bean flour or isolated starch.					
33484150	9	0	theme	±	1592:1592	arg1	SD					1594:1595	[mean ± SD	1586:1595	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	0	theme	±	1592:1592	arg1	reduction					1612:1620	56.1% ± 20.7% reduction	1598:1620	56.1% ± 20.7% reduction	1598:1620	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	11	1	theme	mouse	2258:2262	arg1	models					2264:2269	all mouse models	2254:2269	all mouse models studied	2254:2277	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	6	2	theme	SAMP1/YitFC	1075:1085	arg1	SAMP					1093:1096	SAMP	1093:1096	SAMP	1093:1096	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	2	theme	SAMP1/YitFC	1075:1085	arg1	mice					1087:1090	8-wk-old germ-free SAMP1/YitFC mice	1056:1090	8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors	1056:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	3	theme	Crohn	377:381	arg1	disease					355:361	inflammatory bowel disease	336:361	inflammatory bowel disease (IBD)	336:367	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	3	theme	Crohn	377:381	arg1	CD					392:393	CD	392:393	CD	392:393	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	3	theme	Crohn	377:381	arg1	disease					383:389	Crohn disease	377:389	Crohn disease (CD)	377:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	5	4	theme	healthier	992:1000	arg1	macronutrients					1003:1016	all 3 "healthier" macronutrients	985:1016	all 3 "healthier" macronutrients combined	985:1025	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	5	dep	[redistribution	905:919	arg1	1:1					967:969	1:1	967:969	1:1	967:969	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	5	dep	[redistribution	905:919	arg1	ratio					951:955	ω-3 (n-6:n-3) PUFA ratio	932:955	AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1]	898:970	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	6	6	theme	8-wk-old	1056:1063	arg1	SAMP					1093:1096	SAMP	1093:1096	SAMP	1093:1096	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	6	theme	8-wk-old	1056:1063	arg1	mice					1087:1090	8-wk-old germ-free SAMP1/YitFC mice	1056:1090	8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors	1056:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	11	7	theme	daily	2333:2337	arg1	diet					2345:2348	the daily human diet	2329:2348	the daily human diet	2329:2348	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	4	8	theme	dietary	732:738	arg1	source					740:745	1 macronutrient dietary source	716:745	1 macronutrient dietary source	716:745	Each diet had 1 macronutrient dietary source replaced.					
33484150	9	9	theme	%	1602:1602	arg1	SD					1594:1595	[mean ± SD	1586:1595	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	9	theme	%	1602:1602	arg1	reduction					1612:1620	56.1% ± 20.7% reduction	1598:1620	56.1% ± 20.7% reduction	1598:1620	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	3	10	theme	rodent	623:628	arg1	diets					630:634	5 rodent diets	621:634	5 rodent diets designed to mirror the 2011-2012 "What We Eat in America" NHANES	621:699	METHODS We compared 5 rodent diets designed to mirror the 2011-2012 "What We Eat in America" NHANES.					
33484150	11	11	from	Replacement	2148:2158	arg1	AD					2184:2185	an AD	2181:2185	an AD	2181:2185	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	10	12	theme	acid	1953:1956	arg1	concentrations					2023:2036	butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations	1945:2036	butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations	1945:2036	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	9	13	theme	%	1610:1610	arg1	SD					1594:1595	[mean ± SD	1586:1595	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	13	theme	%	1610:1610	arg1	reduction					1612:1620	56.1% ± 20.7% reduction	1598:1620	56.1% ± 20.7% reduction	1598:1620	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	1	14	theme	nutritional	181:191	arg1	composition					193:203	The current nutritional composition	169:203	The current nutritional composition of the "American diet" (AD; also known as Western diet)	169:259	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	10	15	dep	4:0	1959:1961	arg1	3.3 ± 0.5					1964:1972	3.3 ± 0.5	1964:1972	3.3 ± 0.5	1964:1972	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	15	dep	4:0	1959:1961	arg1	P = 0.006					2012:2020	P = 0.006	2012:2020	P = 0.006	2012:2020	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	7	16	theme	20-wk-old	1369:1377	arg1	mice					1380:1383	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	mice	1380:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	6	17	theme	gut	1120:1122	arg1	"					1145:1145	"hGF-SAMP"	1136:1145	"hGF-SAMP"	1136:1145	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	17	theme	gut	1120:1122	arg1	microbiota					1124:1133	human gut microbiota	1114:1133	human gut microbiota ("hGF-SAMP") from CD or healthy donors	1114:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	18	theme	inflammatory	336:347	arg1	disease					355:361	inflammatory bowel disease	336:361	inflammatory bowel disease (IBD)	336:367	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	18	theme	inflammatory	336:347	arg1	disease					383:389	Crohn disease	377:389	Crohn disease (CD)	377:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	18	theme	inflammatory	336:347	arg1	IBD					364:366	IBD	364:366	IBD	364:366	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	19	attach	linked	270:275	arg1	incidence					295:303	the increasing incidence	280:303	the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD)	280:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	19	attach	linked	270:275	arg2	composition					193:203	The current nutritional composition	169:203	The current nutritional composition of the "American diet" (AD; also known as Western diet)	169:259	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	8	20	theme	Intestinal	1449:1458	arg1	inflammation					1460:1471	Intestinal inflammation	1449:1471	Intestinal inflammation	1449:1471	Intestinal inflammation, gut metagenomics, and MS profiles were assessed.					
33484150	7	21	theme	C57BL/6	1360:1366	arg1	mice					1380:1383	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	mice	1380:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	22	theme	SPF	1355:1357	arg1	mice					1380:1383	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	mice	1380:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	9	23	theme	feeding	1631:1637	arg1	trials					1639:1644	all feeding trials	1627:1644	all feeding trials	1627:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	2	24	from	inflammation	554:565	arg1	models					593:598	experimental IBD mouse models	570:598	experimental IBD mouse models	570:598	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	6	25	from	donors	1167:1172	arg1	"					1145:1145	"hGF-SAMP"	1136:1145	"hGF-SAMP"	1136:1145	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	25	from	donors	1167:1172	arg1	microbiota					1124:1133	human gut microbiota	1114:1133	human gut microbiota ("hGF-SAMP") from CD or healthy donors	1114:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	5	26	theme	animal	799:804	arg1	protein					806:812	animal protein	799:812	animal protein replaced by soy + pea protein	799:842	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	7	27	theme	specific-pathogen-free	1331:1352	arg1	mice					1380:1383	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	mice	1380:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	1	28	dep	known	238:242	arg1	also					233:236	also	233:236	also	233:236	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	9	29	theme	mouse	1654:1658	arg1	models					1660:1665	IBD mouse models	1650:1665	IBD mouse models	1650:1665	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	6	30	theme	CD	1153:1154	arg1	donors					1167:1172	CD or healthy donors	1153:1172	donors	1167:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	7	31	theme	SPF	1424:1426	arg1	SAMP					1428:1431	24-wk-old SPF SAMP	1414:1431	24-wk-old SPF SAMP	1414:1431	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	0	32	from	Protein	17:23	arg1	"					66:66	an "American Diet"	49:66	an "American Diet"	49:66	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	9	33	dep	RESULTS	1523:1529	arg1	resulted					1551:1558	resulted	1551:1558	resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05)	1551:1697	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	0	34	theme	Diet	62:65	arg1	"					66:66	an "American Diet"	49:66	an "American Diet"	49:66	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	11	35	theme	plant-based	2190:2200	arg1	sources					2202:2208	plant-based sources	2190:2208	plant-based sources	2190:2208	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	9	36	theme	other	1676:1680	arg1	diets					1682:1686	other diets	1676:1686	other diets (P < 0.05)	1676:1697	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	36	theme	other	1676:1680	arg1	P < 0.05					1689:1696	P < 0.05	1689:1696	P < 0.05	1689:1696	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	6	37	theme	hGF-SAMP	1137:1144	arg1	"					1145:1145	"hGF-SAMP"	1136:1145	"hGF-SAMP"	1136:1145	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	37	theme	hGF-SAMP	1137:1144	arg1	microbiota					1124:1133	human gut microbiota	1114:1133	human gut microbiota ("hGF-SAMP") from CD or healthy donors	1114:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	38	theme	chronic	308:314	arg1	disease					355:361	inflammatory bowel disease	336:361	inflammatory bowel disease (IBD)	336:367	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	38	theme	chronic	308:314	arg1	diseases					316:323	chronic diseases	308:323	chronic diseases	308:323	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	2	39	dep	OBJECTIVES	397:406	arg1	investigated					419:430	investigated	419:430	investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models	419:598	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	7	40	theme	feeding	1397:1403	arg1	trial					1405:1409	a 6-wk feeding trial	1390:1409	a 6-wk feeding trial in 24-wk-old SPF SAMP	1390:1431	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	41	theme	sulfate-colitis	1275:1289	arg1	experiments					1291:1301	Two subsequent dextran sodium sulfate-colitis experiments	1245:1301	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1245:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	10	42	theme	linoleic	2053:2060	arg1	acid					2062:2065	linoleic acid	2053:2065	linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01)	2053:2133	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	11	43	theme	protein	2170:2176	arg1	Replacement					2148:2158	CONCLUSIONS Replacement	2136:2158	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources	2136:2208	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	2	44	theme	chronic	546:552	arg1	inflammation					554:565	chronic inflammation	546:565	chronic inflammation in experimental IBD mouse models	546:598	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	0	45	theme	Crohn	84:88	arg1	Ileitis					103:109	Murine Crohn Disease-Like Ileitis	77:109	Murine Crohn Disease-Like Ileitis	77:109	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	10	46	dep	18:2n-6	2068:2074	arg1	5.4 ± 0.4					2077:2085	5.4 ± 0.4	2077:2085	5.4 ± 0.4	2077:2085	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	47	theme	3.9 ± 0.3 ng/μg	1906:1920	arg1	stool					1922:1926	3.9 ± 0.3 ng/μg stool	1906:1926	3.9 ± 0.3 ng/μg stool	1906:1926	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	0	48	theme	Replacing	0:8	arg1	Protein					17:23	Replacing Animal Protein	0:23	Replacing Animal Protein with Soy-Pea Protein in an "American Diet"	0:66	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	3	49	dep	METHODS	601:607	arg1	compared					612:619	compared	612:619	compared 5 rodent diets designed to mirror the 2011-2012 "What We Eat in America" NHANES	612:699	METHODS We compared 5 rodent diets designed to mirror the 2011-2012 "What We Eat in America" NHANES.					
33484150	5	50	theme	soy + pea	826:834	arg1	protein					836:842	soy + pea protein	826:842	soy + pea protein	826:842	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	2	51	theme	IBD	583:585	arg1	models					593:598	experimental IBD mouse models	570:598	experimental IBD mouse models	570:598	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	7	52	theme	subsequent	1249:1258	arg1	experiments					1291:1301	Two subsequent dextran sodium sulfate-colitis experiments	1245:1301	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1245:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	10	53	from	abundance	1763:1771	arg1	Leuconostraceae					1797:1811	Leuconostraceae	1797:1811	Leuconostraceae	1797:1811	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	53	from	abundance	1763:1771	arg1	Lactobacillaceae					1776:1791	Lactobacillaceae	1776:1791	Lactobacillaceae	1776:1791	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	53	from	abundance	1763:1771	arg1	concentrations					2023:2036	butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations	1945:2036	butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations	1945:2036	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	53	from	abundance	1763:1771	arg1	glutamine					1871:1879	glutamine	1871:1879	glutamine	1871:1879	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	0	54	theme	Soy-Pea	30:36	arg1	Protein					38:44	Soy-Pea Protein	30:44	Soy-Pea Protein	30:44	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	10	55	dep	acid	2062:2065	arg1	18:2n-6					2068:2074	18:2n-6	2068:2074	18:2n-6	2068:2074	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	55	dep	acid	2062:2065	arg1	P = 0.01					2125:2132	P = 0.01	2125:2132	P = 0.01	2125:2132	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	56	theme	8.6 ± 0.3 ng/μL	2101:2115	arg1	plasma					2117:2122	8.6 ± 0.3 ng/μL plasma	2101:2122	8.6 ± 0.3 ng/μL plasma	2101:2122	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	1	57	dep	BACKGROUND	158:167	arg1	linked					270:275	linked	270:275	has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD)	261:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	0	58	with	Protein	17:23	arg1	Protein					38:44	Soy-Pea Protein	30:44	Soy-Pea Protein	30:44	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	6	59	theme	healthy	1159:1165	arg1	donors					1167:1172	CD or healthy donors	1153:1172	donors	1167:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	5	60	theme	AD-fat	898:903	arg1	[redistribution					905:919	AD-fat [redistribution	898:919	AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1]	898:970	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	6	61	theme	laboratory	1211:1220	arg1	diet					1229:1232	an AD-"modified," or laboratory rodent diet	1190:1232	an AD-"modified," or laboratory rodent diet for 24 wk	1190:1242	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	62	theme	American	213:220	arg1	"					226:226	the "American diet"	208:226	the "American diet" (AD; also known as Western diet)	208:259	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	5	63	theme	carbohydrate	863:874	arg1	"					875:875	"refined carbohydrate"	854:875	"refined carbohydrate" by polysaccharides	854:894	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	11	64	from	IBD	2377:2379	arg1	humans					2384:2389	humans	2384:2389	humans	2384:2389	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	9	65	theme	histology	1569:1577	arg1	scores					1579:1584	histology scores	1569:1584	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	11	66	theme	IBD	2247:2249	arg1	severity					2222:2229	the severity	2218:2229	the severity of experimental IBD in all mouse models studied	2218:2277	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	7	67	from	experiments	1291:1301	arg1	SAMP					1428:1431	24-wk-old SPF SAMP	1414:1431	24-wk-old SPF SAMP	1414:1431	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	67	from	experiments	1291:1301	arg1	mice					1380:1383	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	mice	1380:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	67	from	experiments	1291:1301	arg1	hGF-SAMP					1306:1313	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	hGF-SAMP	1306:1313	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	6	68	theme	"	1206:1206	arg1	diet					1229:1232	an AD-"modified," or laboratory rodent diet	1190:1232	an AD-"modified," or laboratory rodent diet for 24 wk	1190:1242	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	69	theme	"	226:226	arg1	composition					193:203	The current nutritional composition	169:203	The current nutritional composition of the "American diet" (AD; also known as Western diet)	169:259	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	10	70	dep	Lactobacillaceae	1776:1791	arg1	log2					1822:1825	1.5-4.7 log2	1814:1825	1.5-4.7 log2	1814:1825	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	70	dep	Lactobacillaceae	1776:1791	arg1	6.5 ± 0.8					1882:1890	6.5 ± 0.8	1882:1890	6.5 ± 0.8	1882:1890	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	70	dep	Lactobacillaceae	1776:1791	arg1	P = 0.0005					1929:1938	P = 0.0005	1929:1938	P = 0.0005	1929:1938	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	70	dep	Lactobacillaceae	1776:1791	arg1	difference					1844:1853	3.0-5.1 log2 difference	1831:1853	3.0-5.1 log2 difference	1831:1853	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	9	71	theme	[mean	1586:1590	arg1	SD					1594:1595	[mean ± SD	1586:1595	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	71	theme	[mean	1586:1590	arg1	reduction					1612:1620	56.1% ± 20.7% reduction	1598:1620	56.1% ± 20.7% reduction	1598:1620	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	6	72	theme	modified	1197:1204	arg1	diet					1229:1232	an AD-"modified," or laboratory rodent diet	1190:1232	an AD-"modified," or laboratory rodent diet for 24 wk	1190:1242	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	11	73	theme	CONCLUSIONS	2136:2146	arg1	Replacement					2148:2158	CONCLUSIONS Replacement	2136:2158	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources	2136:2208	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	6	74	theme	germ-free	1065:1073	arg1	SAMP					1093:1096	SAMP	1093:1096	SAMP	1093:1096	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	74	theme	germ-free	1065:1073	arg1	mice					1087:1090	8-wk-old germ-free SAMP1/YitFC mice	1056:1090	8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors	1056:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	75	theme	AD-	1193:1195	arg1	diet					1229:1232	an AD-"modified," or laboratory rodent diet	1190:1232	an AD-"modified," or laboratory rodent diet for 24 wk	1190:1242	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	5	76	theme	"	1001:1001	arg1	macronutrients					1003:1016	all 3 "healthier" macronutrients	985:1016	all 3 "healthier" macronutrients combined	985:1025	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	11	77	dep	similar	2296:2302	arg1	feasible					2305:2312	feasible	2305:2312	feasible	2305:2312	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	10	78	theme	increased	1753:1761	arg1	abundance					1763:1771	increased abundance	1753:1771	increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations	1753:2036	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	5	79	theme	formulated	761:770	arg1	AD					783:784	AD	783:784	AD	783:784	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	79	theme	formulated	761:770	arg1	diets					772:776	The formulated diets	757:776	The formulated diets	757:776	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	4	80	theme	macronutrient	718:730	arg1	source					740:745	1 macronutrient dietary source	716:745	1 macronutrient dietary source	716:745	Each diet had 1 macronutrient dietary source replaced.					
33484150	9	81	theme	 ± 20.7	1603:1609	arg1	SD					1594:1595	[mean ± SD	1586:1595	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	81	theme	 ± 20.7	1603:1609	arg1	reduction					1612:1620	56.1% ± 20.7% reduction	1598:1620	56.1% ± 20.7% reduction	1598:1620	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	6	82	theme	separate	1034:1041	arg1	experiments					1043:1053	3 separate experiments	1032:1053	3 separate experiments	1032:1053	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	83	theme	current	173:179	arg1	composition					193:203	The current nutritional composition	169:203	The current nutritional composition of the "American diet" (AD; also known as Western diet)	169:259	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	2	84	theme	greatest	516:523	arg1	impact					525:530	the greatest impact	512:530	the greatest impact	512:530	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	10	85	theme	3.0-5.1	1831:1837	arg1	difference					1844:1853	3.0-5.1 log2 difference	1831:1853	3.0-5.1 log2 difference	1831:1853	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	1	86	dep	"	226:226	arg1	known					238:242	known	238:242	known as Western diet	238:258	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	6	87	theme	human	1114:1118	arg1	"					1145:1145	"hGF-SAMP"	1136:1145	"hGF-SAMP"	1136:1145	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	6	87	theme	human	1114:1118	arg1	microbiota					1124:1133	human gut microbiota	1114:1133	human gut microbiota ("hGF-SAMP") from CD or healthy donors	1114:1172	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	1	88	theme	bowel	349:353	arg1	disease					355:361	inflammatory bowel disease	336:361	inflammatory bowel disease (IBD)	336:367	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	88	theme	bowel	349:353	arg1	disease					383:389	Crohn disease	377:389	Crohn disease (CD)	377:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	1	88	theme	bowel	349:353	arg1	IBD					364:366	IBD	364:366	IBD	364:366	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	10	89	theme	log2	1839:1842	arg1	difference					1844:1853	3.0-5.1 log2 difference	1831:1853	3.0-5.1 log2 difference	1831:1853	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	0	90	theme	Bacteroidetes	137:149	arg1	Ratio					151:155	Bacteroidetes Ratio	137:155	Firmicutes: Bacteroidetes Ratio	125:155	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	11	91	theme	human	2339:2343	arg1	diet					2345:2348	the daily human diet	2329:2348	the daily human diet	2329:2348	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	8	92	theme	gut	1474:1476	arg1	metagenomics					1478:1489	gut metagenomics	1474:1489	gut metagenomics	1474:1489	Intestinal inflammation, gut metagenomics, and MS profiles were assessed.					
33484150	7	93	theme	12-wk-old	1316:1324	arg1	hGF-SAMP					1306:1313	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	hGF-SAMP	1306:1313	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	10	94	theme	2.54 ± 0.4 ng/μg	1988:2003	arg1	stool					2005:2009	2.54 ± 0.4 ng/μg stool	1988:2009	2.54 ± 0.4 ng/μg stool	1988:2009	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	2	95	from	macronutrients	453:466	arg1	AD					505:506	the AD	501:506	the AD	501:506	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	9	96	theme	IBD	1650:1652	arg1	models					1660:1665	IBD mouse models	1650:1665	IBD mouse models	1650:1665	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	5	97	dep	AD-CHO	846:851	arg1	"					875:875	"refined carbohydrate"	854:875	"refined carbohydrate" by polysaccharides	854:894	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	2	98	contain	has	508:510	arg1	which					432:436	which	432:436	which	432:436	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	2	98	contain	has	508:510	arg2	impact					525:530	the greatest impact	512:530	the greatest impact	512:530	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	0	99	theme	American	53:60	arg1	Diet					62:65	American Diet	53:65	an "American Diet"	49:66	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	5	100	theme	ω-6	928:930	arg1	[redistribution					905:919	AD-fat [redistribution	898:919	AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1]	898:970	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	100	theme	ω-6	928:930	arg1	AD-mix					977:982	AD-mix	977:982	AD-mix (all 3 "healthier" macronutrients combined)	977:1026	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	100	theme	ω-6	928:930	arg1	AD-CHO					846:851	AD-CHO	846:851	AD-CHO ("refined carbohydrate" by polysaccharides)	846:895	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	100	theme	ω-6	928:930	arg1	AD					783:784	AD	783:784	AD	783:784	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	100	theme	ω-6	928:930	arg1	AD-soy-pea					787:796	AD-soy-pea	787:796	AD-soy-pea (animal protein replaced by soy + pea protein)	787:843	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	100	theme	ω-6	928:930	arg1	diets					772:776	The formulated diets	757:776	The formulated diets	757:776	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	0	101	dep	Firmicutes	125:134	arg1	Ratio					151:155	Bacteroidetes Ratio	137:155	Firmicutes: Bacteroidetes Ratio	125:155	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	7	102	from	trial	1405:1409	arg1	SAMP					1428:1431	24-wk-old SPF SAMP	1414:1431	24-wk-old SPF SAMP	1414:1431	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	102	from	trial	1405:1409	arg1	mice					1380:1383	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	mice	1380:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	102	from	trial	1405:1409	arg1	hGF-SAMP					1306:1313	hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1306:1383	hGF-SAMP	1306:1313	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	11	103	theme	control/prevent	2361:2375	arg1	IBD					2377:2379	control/prevent IBD	2361:2379	control/prevent IBD in humans	2361:2389	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	1	104	theme	increasing	284:293	arg1	incidence					295:303	the increasing incidence	280:303	the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD)	280:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	8	105	theme	MS	1496:1497	arg1	profiles					1499:1506	MS profiles	1496:1506	MS profiles	1496:1506	Intestinal inflammation, gut metagenomics, and MS profiles were assessed.					
33484150	5	106	dep	AD-mix	977:982	arg1	macronutrients					1003:1016	all 3 "healthier" macronutrients	985:1016	all 3 "healthier" macronutrients combined	985:1025	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	2	107	theme	experimental	570:581	arg1	models					593:598	experimental IBD mouse models	570:598	experimental IBD mouse models	570:598	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	0	108	theme	Murine	77:82	arg1	Ileitis					103:109	Murine Crohn Disease-Like Ileitis	77:109	Murine Crohn Disease-Like Ileitis	77:109	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	7	109	theme	24-wk-old	1414:1422	arg1	SAMP					1428:1431	24-wk-old SPF SAMP	1414:1431	24-wk-old SPF SAMP	1414:1431	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	7	110	theme	sodium	1268:1273	arg1	experiments					1291:1301	Two subsequent dextran sodium sulfate-colitis experiments	1245:1301	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1245:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	2	111	theme	major	447:451	arg1	macronutrients					453:466	the 3 major macronutrients	441:466	the 3 major macronutrients (protein, fat, carbohydrates) in the AD	441:506	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	11	112	theme	animal	2163:2168	arg1	protein					2170:2176	animal protein	2163:2176	animal protein	2163:2176	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	0	113	theme	Disease-Like	90:101	arg1	Ileitis					103:109	Murine Crohn Disease-Like Ileitis	77:109	Murine Crohn Disease-Like Ileitis	77:109	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	1	114	theme	diseases	316:323	arg1	incidence					295:303	the increasing incidence	280:303	the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD)	280:394	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	9	115	dep	lower	1563:1567	arg1	scores					1579:1584	histology scores	1569:1584	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	115	dep	lower	1563:1567	arg1	SD					1594:1595	[mean ± SD	1586:1595	lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials	1563:1644	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	9	115	dep	lower	1563:1567	arg1	reduction					1612:1620	56.1% ± 20.7% reduction	1598:1620	56.1% ± 20.7% reduction	1598:1620	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	5	116	dep	1:1	967:969	arg1	to					964:965	to	964:965	to	964:965	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	0	117	theme	Animal	10:15	arg1	Protein					17:23	Replacing Animal Protein	0:23	Replacing Animal Protein with Soy-Pea Protein in an "American Diet"	0:66	Replacing Animal Protein with Soy-Pea Protein in an "American Diet" Controls Murine Crohn Disease-Like Ileitis Regardless of Firmicutes: Bacteroidetes Ratio.					
33484150	5	118	theme	PUFA	946:949	arg1	ratio					951:955	ω-3 (n-6:n-3) PUFA ratio	932:955	AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1]	898:970	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	7	119	theme	6-wk	1392:1395	arg1	trial					1405:1409	a 6-wk feeding trial	1390:1409	a 6-wk feeding trial in 24-wk-old SPF SAMP	1390:1431	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	2	120	theme	mouse	587:591	arg1	models					593:598	experimental IBD mouse models	570:598	experimental IBD mouse models	570:598	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	5	121	dep	AD-soy-pea	787:796	arg1	protein					806:812	animal protein	799:812	animal protein replaced by soy + pea protein	799:842	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	5	122	theme	ω-3	932:934	arg1	ratio					951:955	ω-3 (n-6:n-3) PUFA ratio	932:955	AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1]	898:970	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	1	123	theme	Western	247:253	arg1	diet					255:258	Western diet	247:258	Western diet	247:258	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
33484150	2	124	dep	macronutrients	453:466	arg1	carbohydrates					483:495	carbohydrates	483:495	carbohydrates	483:495	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	2	124	dep	macronutrients	453:466	arg1	fat					478:480	fat	478:480	fat	478:480	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	2	124	dep	macronutrients	453:466	arg1	protein					469:475	protein	469:475	protein	469:475	OBJECTIVES This study investigated which of the 3 major macronutrients (protein, fat, carbohydrates) in the AD has the greatest impact on preventing chronic inflammation in experimental IBD mouse models.					
33484150	7	125	theme	dextran	1260:1266	arg1	experiments					1291:1301	Two subsequent dextran sodium sulfate-colitis experiments	1245:1301	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice	1245:1383	Two subsequent dextran sodium sulfate-colitis experiments in hGF-SAMP (12-wk-old) and specific-pathogen-free (SPF) C57BL/6 (20-wk-old) mice, and a 6-wk feeding trial in 24-wk-old SPF SAMP were performed.					
33484150	9	126	theme	AD-soy-pea	1535:1544	arg1	diet					1546:1549	The AD-soy-pea diet	1531:1549	The AD-soy-pea diet	1531:1549	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	4	127	theme	Each	702:705	arg1	diet					707:710	Each diet	702:710	Each diet	702:710	Each diet had 1 macronutrient dietary source replaced.					
33484150	11	128	from	severity	2222:2229	arg1	models					2264:2269	all mouse models	2254:2269	all mouse models studied	2254:2277	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	11	129	theme	similar	2296:2302	arg1	adjustments					2314:2324	similar, feasible adjustments	2296:2324	similar, feasible adjustments to the daily human diet	2296:2348	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	6	130	theme	rodent	1222:1227	arg1	diet					1229:1232	an AD-"modified," or laboratory rodent diet	1190:1232	an AD-"modified," or laboratory rodent diet for 24 wk	1190:1242	In 3 separate experiments, 8-wk-old germ-free SAMP1/YitFC mice (SAMP) colonized with human gut microbiota ("hGF-SAMP") from CD or healthy donors were fed an AD, an AD-"modified," or laboratory rodent diet for 24 wk.					
33484150	5	131	theme	refined	855:861	arg1	"					875:875	"refined carbohydrate"	854:875	"refined carbohydrate" by polysaccharides	854:894	The formulated diets were AD, AD-soy-pea (animal protein replaced by soy + pea protein), AD-CHO ("refined carbohydrate" by polysaccharides), AD-fat [redistribution of the ω-6:ω-3 (n-6:n-3) PUFA ratio; ∼10:1 to 1:1], and AD-mix (all 3 "healthier" macronutrients combined).					
33484150	10	132	theme	butyric	1945:1951	arg1	acid					1953:1956	butyric acid	1945:1956	butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations	1945:2036	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	10	132	theme	butyric	1945:1951	arg1	4:0					1959:1961	4:0	1959:1961	4:0	1959:1961	Compared with the AD, the AD-soy-pea correlated with increased abundance in Lactobacillaceae and Leuconostraceae (1.5-4.7 log2 and 3.0-5.1 log2 difference, respectively), glutamine (6.5 ± 0.8 compared with 3.9 ± 0.3 ng/μg stool, P = 0.0005) and butyric acid (4:0; 3.3 ± 0.5 compared with 2.54 ± 0.4 ng/μg stool, P = 0.006) concentrations, and decreased linoleic acid (18:2n-6; 5.4 ± 0.4 compared with 8.6 ± 0.3 ng/μL plasma, P = 0.01).					
33484150	11	133	theme	experimental	2234:2245	arg1	IBD					2247:2249	experimental IBD	2234:2249	experimental IBD	2234:2249	CONCLUSIONS Replacement of animal protein in an AD by plant-based sources reduced the severity of experimental IBD in all mouse models studied, suggesting that similar, feasible adjustments to the daily human diet could help control/prevent IBD in humans.					
33484150	9	134	dep	did	1672:1674	arg1	than					1667:1670	than	1667:1670	than	1667:1670	RESULTS The AD-soy-pea diet resulted in lower histology scores [mean ± SD (56.1% ± 20.7% reduction)] in all feeding trials and IBD mouse models than did other diets (P < 0.05).					
33484150	1	135	theme	diet	222:225	arg1	"					226:226	the "American diet"	208:226	the "American diet" (AD; also known as Western diet)	208:259	BACKGROUND The current nutritional composition of the "American diet" (AD; also known as Western diet) has been linked to the increasing incidence of chronic diseases, including inflammatory bowel disease (IBD), namely Crohn disease (CD).					
32599574	4	0	theme	cholangiocarcinoma	960:977	arg1	cells					979:983	the intrahepatic cholangiocarcinoma cells	943:983	the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	943:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	1	1	theme	patient-derived	278:292	arg1	cells					308:312	patient-derived primary tumor cells	278:312	patient-derived primary tumor cells	278:312	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	0	2	link	patient-derived	15:29	arg1	model					78:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	4	3	theme	Expression	669:678	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	4	from	cells	1127:1131	arg1	construct					1154:1162	the 3D bioprinted construct	1136:1162	the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1136:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	1	5	theme	tumor	302:306	arg1	cells					308:312	patient-derived primary tumor cells	278:312	patient-derived primary tumor cells	278:312	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	6	theme	phenotype	930:938	arg1	development					887:897	the development	883:897	the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	883:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	3	7	theme	forming	603:609	arg1	ability					611:617	a colony forming ability	594:617	a colony forming ability with high survival rate and active proliferation	594:666	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	1	8	theme	model	192:196	arg1	development					153:163	the development	149:163	the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy	149:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	9	theme	metalloproteinase	738:754	arg1	protein					756:762	matrix metalloproteinase protein	731:762	matrix metalloproteinase protein	731:762	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	1	10	theme	extensive	202:210	arg1	study					212:216	extensive study	202:216	extensive study of tumorigenesis and establishment of personalized therapy	202:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	11	theme	cancer	705:710	arg1	markers					722:728	cancer stem cell markers	705:728	cancer stem cell markers	705:728	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	3	12	theme	survival	629:636	arg1	rate					638:641	high survival rate	624:641	high survival rate	624:641	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	4	13	theme	cell	717:720	arg1	markers					722:728	cancer stem cell markers	705:728	cancer stem cell markers	705:728	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	14	theme	3D	992:993	arg1	microenvironment					1009:1024	the 3D printed tumor microenvironment	988:1024	the 3D printed tumor microenvironment	988:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	15	theme	fibrosis	780:787	arg1	index					790:794	index	790:794	index of liver function	790:812	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	15	theme	fibrosis	780:787	arg1	protein					756:762	matrix metalloproteinase protein	731:762	matrix metalloproteinase protein	731:762	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	15	theme	fibrosis	780:787	arg1	index					765:769	index	765:769	index of tumor fibrosis	765:787	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	15	theme	fibrosis	780:787	arg1	markers					722:728	cancer stem cell markers	705:728	cancer stem cell markers	705:728	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	15	theme	fibrosis	780:787	arg1	markers					696:702	tumor markers	690:702	tumor markers	690:702	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	15	theme	fibrosis	780:787	arg1	proteins					864:871	epithelial-mesenchymal transition regulatory proteins	819:871	epithelial-mesenchymal transition regulatory proteins	819:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	16	theme	tumor	1003:1007	arg1	microenvironment					1009:1024	the 3D printed tumor microenvironment	988:1024	the 3D printed tumor microenvironment	988:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	17	theme	index	790:794	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	18	theme	stem-like	1182:1190	arg1	properties					1192:1201	stem-like properties	1182:1201	stem-like properties	1182:1201	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	0	19	theme	model	78:82	arg1	Bioprinting					0:10	Bioprinting	0:10	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	0:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	5	20	theme	3D	1255:1256	arg1	model					1272:1276	current 3D printed tumor model	1247:1276	current 3D printed tumor model	1247:1276	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	21	from	potential	1234:1242	arg1	development					1285:1295	the development	1281:1295	the development of personalized therapy	1281:1319	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	22	theme	tumor	1266:1270	arg1	model					1272:1276	current 3D printed tumor model	1247:1276	current 3D printed tumor model	1247:1276	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	1	23	theme	vivo-mimicking	171:184	arg1	model					192:196	in vivo-mimicking tumor model	168:196	in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy	168:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	24	theme	epithelial-mesenchymal	819:840	arg1	proteins					864:871	epithelial-mesenchymal transition regulatory proteins	819:871	epithelial-mesenchymal transition regulatory proteins	819:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	25	theme	promising	1224:1232	arg1	potential					1234:1242	the promising potential	1220:1242	the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1220:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	1	26	dep	three-dimensional	345:361	arg1	3D					364:365	3D	364:365	3D	364:365	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	27	theme	regulatory	853:862	arg1	proteins					864:871	epithelial-mesenchymal transition regulatory proteins	819:871	epithelial-mesenchymal transition regulatory proteins	819:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	0	28	theme	anti-cancer	115:125	arg1	testing					132:138	anti-cancer drug testing	115:138	anti-cancer drug testing	115:138	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	4	29	from	phenotype	930:938	arg1	microenvironment					1009:1024	the 3D printed tumor microenvironment	988:1024	the 3D printed tumor microenvironment	988:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	2	30	theme	gelatin-alginate-MatrigelTM	494:520	arg1	system					484:489	a composite hydrogel system	463:489	a composite hydrogel system of gelatin-alginate-MatrigelTM	463:520	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	4	31	theme	liver	799:803	arg1	function					805:812	liver function	799:812	liver function	799:812	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	32	theme	personalized	1300:1311	arg1	therapy					1313:1319	personalized therapy	1300:1319	personalized therapy	1300:1319	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	0	33	dep	establishment	85:97	arg1	Bioprinting					0:10	Bioprinting	0:10	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	0:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	2	34	theme	composite	465:473	arg1	system					484:489	a composite hydrogel system	463:489	a composite hydrogel system of gelatin-alginate-MatrigelTM	463:520	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	0	35	theme	patient-derived	15:29	arg1	model					78:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	5	36	theme	anti-cancer	1060:1070	arg1	resistance					1077:1086	anti-cancer drug resistance	1060:1086	anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1060:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	37	theme	conducive	1355:1363	arg1	drugs					1374:1378	more conducive targeted drugs	1350:1378	more conducive targeted drugs	1350:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	38	theme	3D	1140:1141	arg1	construct					1154:1162	the 3D bioprinted construct	1136:1162	the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1136:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	2	39	theme	pre-designed	527:538	arg1	architecture					545:556	pre-designed grid architecture	527:556	pre-designed grid architecture	527:556	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	0	40	theme	cholangiocarcinoma	53:70	arg1	model					78:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	1	41	theme	establishment	239:251	arg1	study					212:216	extensive study	202:216	extensive study of tumorigenesis and establishment of personalized therapy	202:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	2	42	attach	isolated	419:426	arg2	cells					413:417	Intrahepatic cholangiocarcinoma cells	381:417	Intrahepatic cholangiocarcinoma cells isolated from patient	381:439	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	2	42	attach	isolated	419:426	arg1	patient					433:439	patient	433:439	patient	433:439	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	5	43	theme	cholangiocarcinoma	1108:1125	arg1	cells					1127:1131	the intrahepatic cholangiocarcinoma cells	1091:1131	the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1091:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	44	theme	drugs	1374:1378	arg1	discovery					1337:1345	discovery	1337:1345	discovery of more conducive targeted drugs	1337:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	0	45	theme	tumor	72:76	arg1	model					78:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	4	46	theme	tumor	690:694	arg1	markers					696:702	tumor markers	690:702	tumor markers	690:702	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	2	47	theme	Intrahepatic	381:392	arg1	cells					413:417	Intrahepatic cholangiocarcinoma cells	381:417	Intrahepatic cholangiocarcinoma cells isolated from patient	381:439	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	5	48	theme	printed	1258:1264	arg1	model					1272:1276	current 3D printed tumor model	1247:1276	current 3D printed tumor model	1247:1276	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	4	49	theme	intrahepatic	947:958	arg1	cells					979:983	the intrahepatic cholangiocarcinoma cells	943:983	the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	943:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	50	from	resistance	1077:1086	arg1	construct					1154:1162	the 3D bioprinted construct	1136:1162	the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1136:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	1	51	theme	primary	294:300	arg1	cells					308:312	patient-derived primary tumor cells	278:312	patient-derived primary tumor cells	278:312	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	52	theme	cells	979:983	arg1	phenotype					930:938	the invasive and metastatic phenotype	902:938	the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	902:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	1	53	link	patient-derived	278:292	arg1	cells					308:312	patient-derived primary tumor cells	278:312	patient-derived primary tumor cells	278:312	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	3	54	theme	colony	596:601	arg1	ability					611:617	a colony forming ability	594:617	a colony forming ability with high survival rate and active proliferation	594:666	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	1	55	theme	tumor	186:190	arg1	model					192:196	in vivo-mimicking tumor model	168:196	in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy	168:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	56	theme	metastatic	919:928	arg1	phenotype					930:938	the invasive and metastatic phenotype	902:938	the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	902:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	57	theme	markers	722:728	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	58	theme	matrix	731:736	arg1	metalloproteinase					738:754	matrix metalloproteinase	731:754	matrix metalloproteinase protein	731:762	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	3	59	theme	high	624:627	arg1	rate					638:641	high survival rate	624:641	high survival rate	624:641	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	5	60	from	construct	1154:1162	arg1	resistance					1077:1086	anti-cancer drug resistance	1060:1086	anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1060:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	1	61	theme	tumorigenesis	221:233	arg1	study					212:216	extensive study	202:216	extensive study of tumorigenesis and establishment of personalized therapy	202:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	62	theme	stem	712:715	arg1	markers					722:728	cancer stem cell markers	705:728	cancer stem cell markers	705:728	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	63	from	microenvironment	1009:1024	arg1	phenotype					930:938	the invasive and metastatic phenotype	902:938	the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	902:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	3	64	theme	active	647:652	arg1	proliferation					654:666	active proliferation	647:666	active proliferation	647:666	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	4	65	theme	tumor	774:778	arg1	fibrosis					780:787	tumor fibrosis	774:787	tumor fibrosis	774:787	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	0	66	dep	in	31:32	arg1	vitro					34:38	vitro	34:38	vitro	34:38	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	5	67	theme	Similar	1027:1033	arg1	results					1035:1041	Similar results	1027:1041	Similar results	1027:1041	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	4	68	theme	printed	995:1001	arg1	microenvironment					1009:1024	the 3D printed tumor microenvironment	988:1024	the 3D printed tumor microenvironment	988:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	69	theme	protein	756:762	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	70	from	cells	979:983	arg1	microenvironment					1009:1024	the 3D printed tumor microenvironment	988:1024	the 3D printed tumor microenvironment	988:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	1	71	theme	in	168:169	arg1	model					192:196	in vivo-mimicking tumor model	168:196	in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy	168:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	4	72	theme	index	765:769	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	73	theme	model	1272:1276	arg1	potential					1234:1242	the promising potential	1220:1242	the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1220:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	74	theme	drug	1072:1075	arg1	resistance					1077:1086	anti-cancer drug resistance	1060:1086	anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1060:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	0	75	theme	drug	127:130	arg1	testing					132:138	anti-cancer drug testing	115:138	anti-cancer drug testing	115:138	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	4	76	theme	transition	842:851	arg1	proteins					864:871	epithelial-mesenchymal transition regulatory proteins	819:871	epithelial-mesenchymal transition regulatory proteins	819:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	5	77	theme	current	1247:1253	arg1	model					1272:1276	current 3D printed tumor model	1247:1276	current 3D printed tumor model	1247:1276	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	0	78	theme	in	31:32	arg1	model					78:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	5	79	theme	therapy	1313:1319	arg1	development					1285:1295	the development	1281:1295	the development of personalized therapy	1281:1319	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	2	80	theme	hydrogel	475:482	arg1	system					484:489	a composite hydrogel system	463:489	a composite hydrogel system of gelatin-alginate-MatrigelTM	463:520	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	4	81	theme	function	805:812	arg1	index					790:794	index	790:794	index of liver function	790:812	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	81	theme	function	805:812	arg1	protein					756:762	matrix metalloproteinase protein	731:762	matrix metalloproteinase protein	731:762	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	81	theme	function	805:812	arg1	index					765:769	index	765:769	index of tumor fibrosis	765:787	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	81	theme	function	805:812	arg1	markers					722:728	cancer stem cell markers	705:728	cancer stem cell markers	705:728	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	81	theme	function	805:812	arg1	markers					696:702	tumor markers	690:702	tumor markers	690:702	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	4	81	theme	function	805:812	arg1	proteins					864:871	epithelial-mesenchymal transition regulatory proteins	819:871	epithelial-mesenchymal transition regulatory proteins	819:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	0	82	theme	intrahepatic	40:51	arg1	model					78:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	patient-derived in vitro intrahepatic cholangiocarcinoma tumor model	15:82	Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing.					
32599574	2	83	theme	grid	540:543	arg1	architecture					545:556	pre-designed grid architecture	527:556	pre-designed grid architecture	527:556	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	1	84	theme	personalized	256:267	arg1	therapy					269:275	personalized therapy	256:275	personalized therapy	256:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	3	85	with	ability	611:617	arg1	rate					638:641	high survival rate	624:641	high survival rate	624:641	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	3	85	with	ability	611:617	arg1	proliferation					654:666	active proliferation	647:666	active proliferation	647:666	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	5	86	theme	bioprinted	1143:1152	arg1	construct					1154:1162	the 3D bioprinted construct	1136:1162	the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1136:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	4	87	theme	invasive	906:913	arg1	phenotype					930:938	the invasive and metastatic phenotype	902:938	the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment	902:1024	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	1	88	theme	three-dimensional	345:361	arg1	bioprinting					368:378	three-dimensional (3D) bioprinting	345:378	three-dimensional (3D) bioprinting	345:378	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	5	89	theme	intrahepatic	1095:1106	arg1	cells					1127:1131	the intrahepatic cholangiocarcinoma cells	1091:1131	the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1091:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	4	90	theme	proteins	864:871	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	3	91	theme	ICC	559:561	arg1	cells					563:567	ICC cells	559:567	ICC cells	559:567	ICC cells were observed to process a colony forming ability with high survival rate and active proliferation.					
32599574	5	92	theme	targeted	1365:1372	arg1	drugs					1374:1378	more conducive targeted drugs	1350:1378	more conducive targeted drugs	1350:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	5	93	theme	cells	1127:1131	arg1	resistance					1077:1086	anti-cancer drug resistance	1060:1086	anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs	1060:1378	Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.					
32599574	4	94	theme	markers	696:702	arg1	levels					680:685	Expression levels	669:685	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins	669:871	Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment.					
32599574	2	95	theme	cholangiocarcinoma	394:411	arg1	cells					413:417	Intrahepatic cholangiocarcinoma cells	381:417	Intrahepatic cholangiocarcinoma cells isolated from patient	381:439	Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture.					
32599574	1	96	theme	therapy	269:275	arg1	establishment					239:251	establishment	239:251	establishment of personalized therapy	239:275	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32599574	1	96	theme	therapy	269:275	arg1	tumorigenesis					221:233	tumorigenesis	221:233	tumorigenesis	221:233	Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting.					
32719049	0	0	theme	protection	82:91	arg1	systems					93:99	clade-related protection systems	68:99	clade-related protection systems in apple snail eggs	68:119	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	4	1	theme	ca	476:477	arg1	256 kDa					479:485	ca 256 kDa	476:485	ca 256 kDa	476:485	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	5	2	theme	structural	624:633	arg1	stability					635:643	structural stability	624:643	structural stability between pH 2.0 and pH 12.0, and up to 85°C	624:686	It resists in vitro proteinase digestion and displays structural stability between pH 2.0 and pH 12.0, and up to 85°C.					
32719049	1	3	theme	protection	145:154	arg1	vital					159:163	vital	159:163	vital	159:163	The acquisition of egg protection is vital for species survival.					
32719049	1	3	theme	protection	145:154	arg1	acquisition					126:136	The acquisition	122:136	The acquisition of egg protection	122:154	The acquisition of egg protection is vital for species survival.					
32719049	11	4	theme	convergent	1718:1727	arg1	evolution					1729:1737	convergent evolution	1718:1737	convergent evolution	1718:1737	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	0	5	theme	clade-related	68:80	arg1	systems					93:99	clade-related protection systems	68:99	clade-related protection systems in apple snail eggs	68:119	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	10	6	theme	bridgesii	1370:1378	arg1	clade					1380:1384	the bridgesii clade	1366:1384	the bridgesii clade	1366:1384	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	6	7	theme	carotenoids	776:786	arg1	pattern					755:761	glycosylation pattern	741:761	glycosylation pattern	741:761	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	6	7	theme	carotenoids	776:786	arg1	size					789:792	size	789:792	size	789:792	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	6	7	theme	carotenoids	776:786	arg1	presence					764:771	presence	764:771	presence of carotenoids	764:786	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	6	7	theme	carotenoids	776:786	arg1	properties					695:704	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape	689:809	properties	695:704	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	6	7	theme	carotenoids	776:786	arg1	shape					805:809	global shape	798:809	global shape	798:809	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	6	7	theme	carotenoids	776:786	arg1	sequences					730:738	its subunit sequences	718:738	its subunit sequences	718:738	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	4	8	theme	native	445:450	arg1	oligomer					464:471	an oligomer	461:471	an oligomer of ca 256 kDa	461:485	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	4	8	theme	native	445:450	arg1	protein					452:458	The native protein	441:458	The native protein	441:458	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	10	9	dep	value	1460:1464	arg1	lower					1438:1442	lower	1438:1442	lower	1438:1442	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	10	10	with	contrast	1478:1485	arg1	protection					1492:1501	protection	1492:1501	protection by neurotoxins of other Pomacea clades	1492:1540	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	6	11	theme	glycosylation	741:753	arg1	pattern					755:761	glycosylation pattern	741:761	glycosylation pattern	741:761	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	7	12	theme	canaliculata	896:907	arg1	clade					909:913	the canaliculata clade	892:913	the canaliculata clade	892:913	Furthermore, like members of the canaliculata clade, PdPV1 is recovered unchanged in feces of mice ingesting it, supporting an anti-nutritive defensive function.					
32719049	6	13	theme	other	848:852	arg1	Pomacea					854:860	other Pomacea	848:860	other Pomacea	848:860	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	5	14	theme	in	581:582	arg1	digestion					601:609	in vitro proteinase digestion	581:609	in vitro proteinase digestion	581:609	It resists in vitro proteinase digestion and displays structural stability between pH 2.0 and pH 12.0, and up to 85°C.					
32719049	10	15	from	value	1460:1464	arg1	contrast					1478:1485	contrast	1478:1485	contrast with protection by neurotoxins of other Pomacea clades	1478:1540	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	0	16	theme	snail	110:114	arg1	eggs					116:119	apple snail eggs	104:119	apple snail eggs	104:119	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	5	17	theme	proteinase	590:599	arg1	digestion					601:609	in vitro proteinase digestion	581:609	in vitro proteinase digestion	581:609	It resists in vitro proteinase digestion and displays structural stability between pH 2.0 and pH 12.0, and up to 85°C.					
32719049	7	18	theme	clade	909:913	arg1	members					881:887	members	881:887	members of the canaliculata clade	881:913	Furthermore, like members of the canaliculata clade, PdPV1 is recovered unchanged in feces of mice ingesting it, supporting an anti-nutritive defensive function.					
32719049	4	19	dep	has	488:490	arg1	denaturing					551:560	denaturing	551:560	denaturing by SDS	551:567	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	4	19	dep	has	488:490	arg1	withstanding					519:530	withstanding	519:530	withstanding 15 min boiling	519:545	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	10	20	theme	egg	1571:1573	arg1	strategies					1585:1594	apple snail egg defensive strategies	1559:1594	apple snail egg defensive strategies	1559:1594	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	3	21	theme	protective	420:429	arg1	cocktail					431:438	its protective cocktail	416:438	its protective cocktail	416:438	Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail.					
32719049	0	22	theme	apple	104:108	arg1	eggs					116:119	apple snail eggs	104:119	apple snail eggs	104:119	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	7	23	theme	mice	957:960	arg1	feces					948:952	feces	948:952	feces of mice ingesting it	948:973	Furthermore, like members of the canaliculata clade, PdPV1 is recovered unchanged in feces of mice ingesting it, supporting an anti-nutritive defensive function.					
32719049	10	24	theme	Pomacea	1527:1533	arg1	clades					1535:1540	other Pomacea clades	1521:1540	other Pomacea clades	1521:1540	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	3	25	theme	cocktail	431:438	arg1	part					408:411	part	408:411	part of its protective cocktail	408:438	Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail.					
32719049	10	26	theme	clades	1535:1540	arg1	neurotoxins					1506:1516	neurotoxins	1506:1516	neurotoxins of other Pomacea clades	1506:1540	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	8	27	theme	selected	1106:1113	arg1	motifs					1127:1132	selected ganglioside motifs	1106:1132	selected ganglioside motifs	1106:1132	PdPV1 also displays a strong hemagglutinating activity, specifically recognizing selected ganglioside motifs with high affinity.					
32719049	6	28	gly	glycosylation	741:753	arg1	carotenoids					776:786	carotenoids	776:786	carotenoids	776:786	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	2	29	from	snails	221:226	arg1	eggs					197:200	Poisonous eggs	187:200	Poisonous eggs from Pomacea apple snails	187:226	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32719049	1	30	theme	species	169:175	arg1	survival					177:184	species survival	169:184	species survival	169:184	The acquisition of egg protection is vital for species survival.					
32719049	3	31	theme	Pomacea	382:388	arg1	diffusa					390:396	Pomacea diffusa	382:396	Pomacea diffusa	382:396	Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail.					
32719049	4	32	theme	15 min	532:537	arg1	boiling					539:545	15 min boiling	532:545	15 min boiling	532:545	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	11	33	theme	predators	1658:1666	arg1	environment					1643:1653	The harsh gastrointestinal environment	1616:1653	The harsh gastrointestinal environment of predators	1616:1666	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	11	34	theme	stable	1773:1778	arg1	lectins					1780:1786	plant-like highly stable lectins	1755:1786	plant-like highly stable lectins	1755:1786	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	8	35	theme	ganglioside	1115:1125	arg1	motifs					1127:1132	selected ganglioside motifs	1106:1132	selected ganglioside motifs	1106:1132	PdPV1 also displays a strong hemagglutinating activity, specifically recognizing selected ganglioside motifs with high affinity.					
32719049	10	36	theme	snail	1565:1569	arg1	strategies					1585:1594	apple snail egg defensive strategies	1559:1594	apple snail egg defensive strategies	1559:1594	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	10	37	theme	other	1521:1525	arg1	clades					1535:1540	other Pomacea clades	1521:1540	other Pomacea clades	1521:1540	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	11	38	theme	plant-like	1755:1764	arg1	lectins					1780:1786	plant-like highly stable lectins	1755:1786	plant-like highly stable lectins	1755:1786	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	10	39	theme	egg	1341:1343	arg1	defenses					1345:1352	their egg defenses	1335:1352	their egg defenses	1335:1352	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	11	40	theme	lectins	1780:1786	arg1	presence					1743:1750	the presence	1739:1750	the presence of plant-like highly stable lectins	1739:1786	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	0	41	theme	stable	9:14	arg1	lectin					32:37	A highly stable, non-digestible lectin	0:37	A highly stable, non-digestible lectin from Pomacea diffusa	0:58	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	11	42	theme	gastrointestinal	1626:1641	arg1	environment					1643:1653	The harsh gastrointestinal environment	1616:1653	The harsh gastrointestinal environment of predators	1616:1666	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	11	43	theme	other	1816:1820	arg1	animals					1822:1828	other animals	1816:1828	other animals	1816:1828	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	10	44	from	species	1289:1295	arg1	Pomacea					1310:1316	the genus Pomacea	1300:1316	the genus Pomacea	1300:1316	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	10	45	theme	apple	1559:1563	arg1	strategies					1585:1594	apple snail egg defensive strategies	1559:1594	apple snail egg defensive strategies	1559:1594	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	7	46	theme	anti-nutritive	990:1003	arg1	function					1015:1022	an anti-nutritive defensive function	987:1022	an anti-nutritive defensive function	987:1022	Furthermore, like members of the canaliculata clade, PdPV1 is recovered unchanged in feces of mice ingesting it, supporting an anti-nutritive defensive function.					
32719049	4	47	theme	structural	497:506	arg1	stability					508:516	high structural stability	492:516	high structural stability	492:516	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	7	48	theme	defensive	1005:1013	arg1	function					1015:1022	an anti-nutritive defensive function	987:1022	an anti-nutritive defensive function	987:1022	Furthermore, like members of the canaliculata clade, PdPV1 is recovered unchanged in feces of mice ingesting it, supporting an anti-nutritive defensive function.					
32719049	6	49	theme	subunit	722:728	arg1	sequences					730:738	its subunit sequences	718:738	its subunit sequences	718:738	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	8	50	theme	high	1139:1142	arg1	affinity					1144:1151	high affinity	1139:1151	high affinity	1139:1151	PdPV1 also displays a strong hemagglutinating activity, specifically recognizing selected ganglioside motifs with high affinity.					
32719049	0	51	theme	Pomacea	44:50	arg1	diffusa					52:58	Pomacea diffusa	44:58	Pomacea diffusa	44:58	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	4	52	theme	high	492:495	arg1	stability					508:516	high structural stability	492:516	high structural stability	492:516	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	8	53	theme	hemagglutinating	1054:1069	arg1	activity					1071:1078	a strong hemagglutinating activity	1045:1078	a strong hemagglutinating activity	1045:1078	PdPV1 also displays a strong hemagglutinating activity, specifically recognizing selected ganglioside motifs with high affinity.					
32719049	0	54	from	diffusa	52:58	arg1	lectin					32:37	A highly stable, non-digestible lectin	0:37	A highly stable, non-digestible lectin from Pomacea diffusa	0:58	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	2	55	theme	apple	215:219	arg1	snails					221:226	Pomacea apple snails	207:226	Pomacea apple snails	207:226	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32719049	8	56	theme	strong	1047:1052	arg1	activity					1071:1078	a strong hemagglutinating activity	1045:1078	a strong hemagglutinating activity	1045:1078	PdPV1 also displays a strong hemagglutinating activity, specifically recognizing selected ganglioside motifs with high affinity.					
32719049	10	57	theme	non-digestible	1410:1423	arg1	lectins					1425:1431	non-digestible lectins	1410:1431	non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades	1410:1540	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	3	58	theme	novel	311:315	arg1	lectin					317:322	a novel lectin	309:322	a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail	309:438	Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail.					
32719049	6	59	theme	global	798:803	arg1	shape					805:809	global shape	798:809	global shape	798:809	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	2	60	theme	Pomacea	207:213	arg1	snails					221:226	Pomacea apple snails	207:226	Pomacea apple snails	207:226	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32719049	11	61	dep	presence	1743:1750	arg1	evolution					1729:1737	convergent evolution	1718:1737	convergent evolution	1718:1737	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	9	62	from	clade	1224:1228	arg1	perivitelline					1196:1208	perivitelline	1196:1208	perivitelline	1196:1208	This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade).					
32719049	9	62	from	clade	1224:1228	arg1	PsSC					1188:1191	PsSC	1188:1191	PsSC	1188:1191	This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade).					
32719049	5	63	dep	in	581:582	arg1	vitro					584:588	vitro	584:588	vitro	584:588	It resists in vitro proteinase digestion and displays structural stability between pH 2.0 and pH 12.0, and up to 85°C.					
32719049	6	64	from	Pomacea	854:860	arg1	orthologs					833:841	its orthologs	829:841	its orthologs from other Pomacea	829:860	These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size and global shape resemble those of its orthologs from other Pomacea.					
32719049	10	65	theme	nutritional	1448:1458	arg1	value					1460:1464	the nutritional value	1444:1464	the nutritional value of eggs	1444:1472	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	9	66	theme	same	1219:1222	arg1	clade					1224:1228	the same clade	1215:1228	the same clade (bridgesii clade)	1215:1246	This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade).					
32719049	9	66	theme	same	1219:1222	arg1	clade					1241:1245	bridgesii clade	1231:1245	bridgesii clade	1231:1245	This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade).					
32719049	2	67	theme	Poisonous	187:195	arg1	eggs					197:200	Poisonous eggs	187:200	Poisonous eggs from Pomacea apple snails	187:226	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32719049	0	68	dep	stable	9:14	arg1	non-digestible					17:30	non-digestible	17:30	non-digestible	17:30	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	10	69	theme	genus	1304:1308	arg1	Pomacea					1310:1316	the genus Pomacea	1300:1316	the genus Pomacea	1300:1316	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	4	70	contain	has	488:490	arg1	oligomer					464:471	an oligomer	461:471	an oligomer of ca 256 kDa	461:485	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	4	70	contain	has	488:490	arg2	stability					508:516	high structural stability	492:516	high structural stability	492:516	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	4	70	contain	has	488:490	arg1	protein					452:458	The native protein	441:458	The native protein	441:458	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	5	71	dep	85°C	683:686	arg1	up					677:678	up	677:678	up	677:678	It resists in vitro proteinase digestion and displays structural stability between pH 2.0 and pH 12.0, and up to 85°C.					
32719049	0	72	from	systems	93:99	arg1	eggs					116:119	apple snail eggs	104:119	apple snail eggs	104:119	A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.					
32719049	10	73	theme	defensive	1575:1583	arg1	strategies					1585:1594	apple snail egg defensive strategies	1559:1594	apple snail egg defensive strategies	1559:1594	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	10	74	theme	eggs	1469:1472	arg1	value					1460:1464	the nutritional value	1444:1464	the nutritional value of eggs	1444:1472	As a whole, these results indicate that species in the genus Pomacea have diversified their egg defenses: those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating that apple snail egg defensive strategies are clade specific.					
32719049	3	75	theme	diffusa	390:396	arg1	eggs					374:377	the eggs	370:377	the eggs of Pomacea diffusa	370:396	Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail.					
32719049	4	76	theme	256 kDa	479:485	arg1	oligomer					464:471	an oligomer	461:471	an oligomer of ca 256 kDa	461:485	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	4	76	theme	256 kDa	479:485	arg1	protein					452:458	The native protein	441:458	The native protein	441:458	The native protein, an oligomer of ca 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by SDS.					
32719049	2	77	theme	defensive	233:241	arg1	macromolecules					243:256	defensive macromolecules	233:256	defensive macromolecules for protection	233:271	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32719049	1	78	theme	egg	141:143	arg1	protection					145:154	egg protection	141:154	egg protection	141:154	The acquisition of egg protection is vital for species survival.					
32719049	11	79	theme	harsh	1620:1624	arg1	environment					1643:1653	The harsh gastrointestinal environment	1616:1653	The harsh gastrointestinal environment of predators	1616:1666	The harsh gastrointestinal environment of predators would have favored their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.					
32719049	2	80	contain	have	228:231	arg2	macromolecules					243:256	defensive macromolecules	233:256	defensive macromolecules for protection	233:271	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32719049	2	80	contain	have	228:231	arg1	eggs					197:200	Poisonous eggs	187:200	Poisonous eggs from Pomacea apple snails	187:226	Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection.					
32766583	5	0	theme	receptor	647:654	arg1	glycosylation					621:633	glycosylation	621:633	glycosylation of the ACE2 receptor	621:654	We further observed that glycosylation of the ACE2 receptor enhances SARS-CoV-2 infectivity.					
32766583	6	1	theme	glycan-protein	754:767	arg1	interactions					769:780	glycan-protein interactions	754:780	glycan-protein interactions	754:780	Importantly estrogens can disrupt glycan-glycan interactions and glycan-protein interactions between the human ACE2 and the SARS-CoV2 thereby blocking its entry into cells.					
32766583	1	2	theme	severe	150:155	arg1	infection					157:165	severe infection	150:165	severe infection by the SARS-CoV-2 virus	150:189	Emerging evidence suggests that males are more susceptible to severe infection by the SARS-CoV-2 virus than females.					
32766583	7	3	theme	alveolar	929:936	arg1	uptake					938:943	alveolar uptake	929:943	alveolar uptake of the SARS-CoV-2 spike protein	929:975	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	4	4	theme	ACE2	581:584	arg1	receptor					586:593	the human ACE2 receptor	571:593	the human ACE2 receptor	571:593	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	0	5	theme	cell	76:79	arg1	entry					81:85	SARS-Cov2 cell entry	66:85	SARS-Cov2 cell entry	66:85	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	8	6	theme	putative	1008:1015	arg1	mechanism					1017:1025	a putative mechanism	1006:1025	a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19	1006:1179	These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.					
32766583	4	7	theme	human	575:579	arg1	receptor					586:593	the human ACE2 receptor	571:593	the human ACE2 receptor	571:593	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	3	8	theme	female	362:367	arg1	hormones					373:380	female sex hormones	362:380	female sex hormones	362:380	However, the precise mechanisms by which female sex hormones may provide protection against SARS-CoV-2 infectivity remains unknown.					
32766583	8	9	theme	female	1035:1040	arg1	hormones					1046:1053	female sex hormones	1035:1053	female sex hormones	1035:1053	These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.					
32766583	5	10	theme	SARS-CoV-2	665:674	arg1	infectivity					676:686	SARS-CoV-2 infectivity	665:686	SARS-CoV-2 infectivity	665:686	We further observed that glycosylation of the ACE2 receptor enhances SARS-CoV-2 infectivity.					
32766583	3	11	theme	sex	369:371	arg1	hormones					373:380	female sex hormones	362:380	female sex hormones	362:380	However, the precise mechanisms by which female sex hormones may provide protection against SARS-CoV-2 infectivity remains unknown.					
32766583	4	12	theme	molecular	490:498	arg1	basis					500:504	the molecular basis	486:504	the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor	486:593	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	7	13	theme	ACE2	898:901	arg1	glycosylation					903:915	ACE2 glycosylation	898:915	ACE2 glycosylation	898:915	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	0	14	theme	sex	45:47	arg1	hormones					49:56	female sex hormones	38:56	female sex hormones against SARS-Cov2 cell entry	38:85	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	8	15	theme	severe	1094:1099	arg1	infection					1101:1109	severe infection	1094:1109	severe infection	1094:1109	These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.					
32766583	8	16	theme	sex	1042:1044	arg1	hormones					1046:1053	female sex hormones	1035:1053	female sex hormones	1035:1053	These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.					
32766583	6	17	theme	glycan-glycan	723:735	arg1	interactions					737:748	glycan-glycan interactions	723:748	glycan-glycan interactions	723:748	Importantly estrogens can disrupt glycan-glycan interactions and glycan-protein interactions between the human ACE2 and the SARS-CoV2 thereby blocking its entry into cells.					
32766583	0	18	theme	SARS-Cov2	66:74	arg1	entry					81:85	SARS-Cov2 cell entry	66:85	SARS-Cov2 cell entry	66:85	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	1	19	theme	SARS-CoV-2	174:183	arg1	virus					185:189	the SARS-CoV-2 virus	170:189	the SARS-CoV-2 virus	170:189	Emerging evidence suggests that males are more susceptible to severe infection by the SARS-CoV-2 virus than females.					
32766583	0	20	theme	Structural	0:9	arg1	analysis					26:33	Structural and functional analysis	0:33	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.	0:86	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	5	21	theme	ACE2	642:645	arg1	receptor					647:654	the ACE2 receptor	638:654	the ACE2 receptor	638:654	We further observed that glycosylation of the ACE2 receptor enhances SARS-CoV-2 infectivity.					
32766583	7	22	theme	spike	963:967	arg1	protein					969:975	the SARS-CoV-2 spike protein	948:975	the SARS-CoV-2 spike protein	948:975	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	1	23	theme	Emerging	88:95	arg1	evidence					97:104	Emerging evidence	88:104	Emerging evidence	88:104	Emerging evidence suggests that males are more susceptible to severe infection by the SARS-CoV-2 virus than females.					
32766583	7	24	theme	mouse	867:871	arg1	model					873:877	a mouse model	865:877	a mouse model	865:877	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	7	25	theme	protein	969:975	arg1	uptake					938:943	alveolar uptake	929:943	alveolar uptake of the SARS-CoV-2 spike protein	929:975	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	0	26	theme	functional	15:24	arg1	analysis					26:33	Structural and functional analysis	0:33	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.	0:86	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	4	27	theme	new	468:470	arg1	insights					472:479	new insights	468:479	new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor	468:593	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	2	28	theme	sex	307:309	arg1	hormones					311:318	sex hormones	307:318	sex hormones	307:318	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	5	29	gly	glycosylation	621:633	arg1	receptor					647:654	the ACE2 receptor	638:654	the ACE2 receptor	638:654	We further observed that glycosylation of the ACE2 receptor enhances SARS-CoV-2 infectivity.					
32766583	0	30	theme	female	38:43	arg1	hormones					49:56	female sex hormones	38:56	female sex hormones against SARS-Cov2 cell entry	38:85	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	4	31	theme	spike	549:553	arg1	protein					559:565	the SARS-CoV-2 spike (S) protein	534:565	the SARS-CoV-2 spike (S) protein	534:565	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	2	32	theme	mechanisms	218:227	arg1	mechanisms					218:227	mechanisms	218:227	mechanisms	218:227	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	2	32	theme	mechanisms	218:227	arg1	variety					207:213	A variety	205:213	A variety of mechanisms	205:227	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	0	33	theme	hormones	49:56	arg1	analysis					26:33	Structural and functional analysis	0:33	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.	0:86	Structural and functional analysis of female sex hormones against SARS-Cov2 cell entry.					
32766583	4	34	theme	SARS-CoV-2	538:547	arg1	protein					559:565	the SARS-CoV-2 spike (S) protein	534:565	the SARS-CoV-2 spike (S) protein	534:565	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	8	35	theme	therapies	1154:1162	arg1	development					1132:1142	the development	1128:1142	the development of future therapies against COVID-19	1128:1179	These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.					
32766583	7	36	gly	glycosylation	903:915	arg1	model					873:877	a mouse model	865:877	a mouse model	865:877	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	3	37	theme	SARS-CoV-2	413:422	arg1	infectivity					424:434	SARS-CoV-2 infectivity	413:434	SARS-CoV-2 infectivity	413:434	However, the precise mechanisms by which female sex hormones may provide protection against SARS-CoV-2 infectivity remains unknown.					
32766583	4	38	theme	S	556:556	arg1	protein					559:565	the SARS-CoV-2 spike (S) protein	534:565	the SARS-CoV-2 spike (S) protein	534:565	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
32766583	8	39	theme	future	1147:1152	arg1	therapies					1154:1162	future therapies	1147:1162	future therapies against COVID-19	1147:1179	These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.					
32766583	2	40	theme	gender-related	255:268	arg1	differences					292:302	differences	292:302	differences in sex hormones	292:318	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	2	40	theme	gender-related	255:268	arg1	disparities					270:280	the observed gender-related disparities	242:280	the observed gender-related disparities including differences in sex hormones	242:318	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	6	41	theme	human	794:798	arg1	ACE2					800:803	the human ACE2	790:803	the human ACE2	790:803	Importantly estrogens can disrupt glycan-glycan interactions and glycan-protein interactions between the human ACE2 and the SARS-CoV2 thereby blocking its entry into cells.					
32766583	7	42	theme	SARS-CoV-2	952:961	arg1	protein					969:975	the SARS-CoV-2 spike protein	948:975	the SARS-CoV-2 spike protein	948:975	In a mouse model, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein.					
32766583	2	43	theme	observed	246:253	arg1	differences					292:302	differences	292:302	differences in sex hormones	292:318	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	2	43	theme	observed	246:253	arg1	disparities					270:280	the observed gender-related disparities	242:280	the observed gender-related disparities including differences in sex hormones	242:318	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	3	44	theme	precise	334:340	arg1	mechanisms					342:351	the precise mechanisms	330:351	the precise mechanisms by which female sex hormones may provide protection against SARS-CoV-2 infectivity	330:434	However, the precise mechanisms by which female sex hormones may provide protection against SARS-CoV-2 infectivity remains unknown.					
32766583	2	45	from	differences	292:302	arg1	hormones					311:318	sex hormones	307:318	sex hormones	307:318	A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones.					
32766583	4	46	theme	interactions	513:524	arg1	basis					500:504	the molecular basis	486:504	the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor	486:593	Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor.					
35007031	0	0	theme	Pathogens	113:121	arg1	Disinfection					76:87	Synergetic Disinfection	65:87	Synergetic Disinfection of Antibiotic-Resistant Pathogens	65:121	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	5	1	mod	modified	741:748	arg3	QCS					777:779	QCS	777:779	QCS	777:779	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	5	1	mod	modified	741:748	arg3	adsorption					799:808	electrostatic adsorption	785:808	electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection	785:897	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	5	1	mod	modified	741:748	arg1	nanosheets					725:734	BP nanosheets	722:734	BP nanosheets	722:734	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	5	1	mod	modified	741:748	arg3	chitosan					767:774	quaternized chitosan	755:774	quaternized chitosan (QCS)	755:780	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	0	2	theme	Antibiotic-Resistant	92:111	arg1	Pathogens					113:121	Antibiotic-Resistant Pathogens	92:121	Antibiotic-Resistant Pathogens	92:121	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	6	3	contain	has	911:913	arg1	BP-QCS					904:909	The BP-QCS	900:909	The BP-QCS	900:909	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	6	3	contain	has	911:913	arg2	solubility					934:943	obviously enhanced solubility	915:943	obviously enhanced solubility	915:943	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	6	3	contain	has	911:913	arg2	stability					958:966	chemical stability	949:966	chemical stability	949:966	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	9	4	dep	in	1457:1458	arg1	vivo					1460:1463	vivo	1460:1463	vivo	1460:1463	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	6	5	from	stability	958:966	arg1	suspensions					979:989	aqueous suspensions	971:989	aqueous suspensions	971:989	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	2	6	theme	great	332:336	arg1	interest					338:345	great interest	332:345	great interest	332:345	Currently, an alternative antibiotic-free therapy for inactivating bacteria is of great interest.					
35007031	3	7	theme	nontoxic	391:398	arg1	material					436:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	7	theme	nontoxic	391:398	arg1	phosphorus					354:363	Black phosphorus	348:363	Black phosphorus (BP)	348:368	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	6	8	theme	aqueous	971:977	arg1	suspensions					979:989	aqueous suspensions	971:989	aqueous suspensions	971:989	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	9	9	from	concentrations	1409:1422	arg1	suspensions					1370:1380	the BP-QCS suspensions	1359:1380	the BP-QCS suspensions at effective antibacterial concentrations	1359:1422	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	7	10	with	min	1207:1209	arg1	dose					1218:1221	a dose	1216:1221	a dose of only 75 μg/mL in vitro	1216:1247	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	4	11	theme	ambient	638:644	arg1	environments					646:657	ambient environments	638:657	ambient environments	638:657	However, BP nanosheets suffer from instability in ambient environments due to rapid oxidation and degradation.					
35007031	7	12	theme	μg/mL	1234:1238	arg1	dose					1218:1221	a dose	1216:1221	a dose of only 75 μg/mL in vitro	1216:1247	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	3	13	theme	cancer	523:528	arg1	therapy					530:536	cancer therapy	523:536	cancer therapy	523:536	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	14	theme	many	480:483	arg1	biosensing					511:520	biosensing	511:520	biosensing	511:520	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	14	theme	many	480:483	arg1	treatment					577:585	antibacterial treatment	563:585	antibacterial treatment	563:585	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	14	theme	many	480:483	arg1	bioapplications					485:499	many bioapplications	480:499	many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment	480:585	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	14	theme	many	480:483	arg1	therapy					530:536	cancer therapy	523:536	cancer therapy	523:536	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	14	theme	many	480:483	arg1	delivery					544:551	drug delivery	539:551	drug delivery	539:551	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	15	theme	biocompatible	373:385	arg1	material					436:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	15	theme	biocompatible	373:385	arg1	phosphorus					354:363	Black phosphorus	348:363	Black phosphorus (BP)	348:368	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	0	16	theme	Stable	2:7	arg1	Nanocomposite					47:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite	0:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.	0:122	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	6	17	theme	chemical	949:956	arg1	stability					958:966	chemical stability	949:966	chemical stability	949:966	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	7	18	theme	near-infrared	1024:1036	arg1	irradiation					1044:1054	near-infrared (NIR) irradiation	1024:1054	near-infrared (NIR) irradiation	1024:1054	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	3	19	theme	two-dimensional	412:426	arg1	material					436:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	19	theme	two-dimensional	412:426	arg1	phosphorus					354:363	Black phosphorus	348:363	Black phosphorus (BP)	348:368	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	9	20	theme	BP-QCS	1363:1368	arg1	suspensions					1370:1380	the BP-QCS suspensions	1359:1380	the BP-QCS suspensions at effective antibacterial concentrations	1359:1422	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	7	21	from	dose	1218:1221	arg1	vitro					1243:1247	vitro	1243:1247	vitro	1243:1247	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	0	22	theme	Chitosan-Black	21:34	arg1	Nanocomposite					47:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite	0:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.	0:122	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	4	23	theme	BP	597:598	arg1	nanosheets					600:609	BP nanosheets	597:609	BP nanosheets	597:609	However, BP nanosheets suffer from instability in ambient environments due to rapid oxidation and degradation.					
35007031	7	24	theme	NIR	1039:1041	arg1	irradiation					1044:1054	near-infrared (NIR) irradiation	1024:1054	near-infrared (NIR) irradiation	1024:1054	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	7	25	dep	%	1111:1111	arg1	95					1109:1110	95	1109:1110	95	1109:1110	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	5	26	theme	electrostatic	785:797	arg1	adsorption					799:808	electrostatic adsorption	785:808	electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection	785:897	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	0	27	theme	Quaternized	9:19	arg1	Nanocomposite					47:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite	0:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.	0:122	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	5	28	theme	quaternized	755:765	arg1	QCS					777:779	QCS	777:779	QCS	777:779	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	5	28	theme	quaternized	755:765	arg1	chitosan					767:774	quaternized chitosan	755:774	quaternized chitosan (QCS)	755:780	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	7	29	from	μg/mL	1234:1238	arg1	vitro					1243:1247	vitro	1243:1247	vitro	1243:1247	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	1	30	used	used	147:150	arg2	Antibiotics					124:134	Antibiotics	124:134	Antibiotics	124:134	Antibiotics are widely used for treatment of bacterial infections, and their overuse has contributed to microbial resistance.					
35007031	1	31	theme	microbial	228:236	arg1	resistance					238:247	microbial resistance	228:247	microbial resistance	228:247	Antibiotics are widely used for treatment of bacterial infections, and their overuse has contributed to microbial resistance.					
35007031	5	32	theme	BP-QCS	823:828	arg1	composite					830:838	a BP-QCS composite	821:838	a BP-QCS composite	821:838	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	9	33	theme	effective	1385:1393	arg1	concentrations					1409:1422	effective antibacterial concentrations	1385:1422	effective antibacterial concentrations	1385:1422	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	2	34	theme	alternative	264:274	arg1	therapy					292:298	an alternative antibiotic-free therapy	261:298	an alternative antibiotic-free therapy for inactivating bacteria	261:324	Currently, an alternative antibiotic-free therapy for inactivating bacteria is of great interest.					
35007031	3	35	theme	remarkable	457:466	arg1	interest					468:475	remarkable interest	457:475	remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment	457:585	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	9	36	theme	antibacterial	1395:1407	arg1	concentrations					1409:1422	effective antibacterial concentrations	1385:1422	effective antibacterial concentrations	1385:1422	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	0	37	theme	Phosphorus	36:45	arg1	Nanocomposite					47:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite	0:59	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.	0:122	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	6	38	theme	enhanced	925:932	arg1	solubility					934:943	obviously enhanced solubility	915:943	obviously enhanced solubility	915:943	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	3	39	theme	rising-star	400:410	arg1	material					436:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	39	theme	rising-star	400:410	arg1	phosphorus					354:363	Black phosphorus	348:363	Black phosphorus (BP)	348:368	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	4	40	from	instability	623:633	arg1	environments					646:657	ambient environments	638:657	ambient environments	638:657	However, BP nanosheets suffer from instability in ambient environments due to rapid oxidation and degradation.					
35007031	0	41	theme	Synergetic	65:74	arg1	Disinfection					76:87	Synergetic Disinfection	65:87	Synergetic Disinfection of Antibiotic-Resistant Pathogens	65:121	A Stable Quaternized Chitosan-Black Phosphorus Nanocomposite for Synergetic Disinfection of Antibiotic-Resistant Pathogens.					
35007031	3	42	theme	Black	348:352	arg1	material					436:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	42	theme	Black	348:352	arg1	BP					366:367	BP	366:367	BP	366:367	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	42	theme	Black	348:352	arg1	phosphorus					354:363	Black phosphorus	348:363	Black phosphorus (BP)	348:368	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	43	theme	drug	539:542	arg1	delivery					544:551	drug delivery	539:551	drug delivery	539:551	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	5	44	theme	photothermal/pharmaco	844:864	arg1	treatment					866:874	photothermal/pharmaco treatment	844:874	photothermal/pharmaco treatment of bacterial infection	844:897	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	5	45	theme	bacterial	879:887	arg1	infection					889:897	bacterial infection	879:897	bacterial infection	879:897	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	7	46	from	vitro	1243:1247	arg1	dose					1218:1221	a dose	1216:1221	a dose of only 75 μg/mL in vitro	1216:1247	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	9	47	theme	negligible	1429:1438	arg1	cytotoxicity					1440:1451	negligible cytotoxicity	1429:1451	negligible cytotoxicity	1429:1451	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	5	48	theme	BP	722:723	arg1	nanosheets					725:734	BP nanosheets	722:734	BP nanosheets	722:734	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	2	49	theme	inactivating	304:315	arg1	bacteria					317:324	inactivating bacteria	304:324	inactivating bacteria	304:324	Currently, an alternative antibiotic-free therapy for inactivating bacteria is of great interest.					
35007031	6	50	from	solubility	934:943	arg1	suspensions					979:989	aqueous suspensions	971:989	aqueous suspensions	971:989	The BP-QCS has obviously enhanced solubility and chemical stability in aqueous suspensions.					
35007031	5	51	theme	infection	889:897	arg1	treatment					866:874	photothermal/pharmaco treatment	844:874	photothermal/pharmaco treatment of bacterial infection	844:897	To address this issue, BP nanosheets were modified with quaternized chitosan (QCS) by electrostatic adsorption to prepare a BP-QCS composite for photothermal/pharmaco treatment of bacterial infection.					
35007031	3	52	from	interest	468:475	arg1	biosensing					511:520	biosensing	511:520	biosensing	511:520	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	52	from	interest	468:475	arg1	treatment					577:585	antibacterial treatment	563:585	antibacterial treatment	563:585	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	52	from	interest	468:475	arg1	bioapplications					485:499	many bioapplications	480:499	many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment	480:585	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	52	from	interest	468:475	arg1	therapy					530:536	cancer therapy	523:536	cancer therapy	523:536	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	52	from	interest	468:475	arg1	delivery					544:551	drug delivery	539:551	drug delivery	539:551	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	1	53	theme	infections	179:188	arg1	treatment					156:164	treatment	156:164	treatment of bacterial infections	156:188	Antibiotics are widely used for treatment of bacterial infections, and their overuse has contributed to microbial resistance.					
35007031	4	54	theme	due	659:661	arg1	instability					623:633	instability	623:633	instability in ambient environments due to rapid oxidation and degradation	623:696	However, BP nanosheets suffer from instability in ambient environments due to rapid oxidation and degradation.					
35007031	7	55	dep	aureus	1150:1155	arg1	%					1111:1111	%	1111:1111	%	1111:1111	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	7	55	dep	aureus	1150:1155	arg1	aureus					1161:1166	S. aureus	1158:1166	S. aureus	1158:1166	We have demonstrated that under near-infrared (NIR) irradiation, the BP-QCS can synergistically inactivate more than 95% methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli within 10 min with a dose of only 75 μg/mL in vitro.					
35007031	9	56	contain	have	1424:1427	arg2	cytotoxicity					1440:1451	negligible cytotoxicity	1429:1451	negligible cytotoxicity	1429:1451	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	9	56	contain	have	1424:1427	arg1	suspensions					1370:1380	the BP-QCS suspensions	1359:1380	the BP-QCS suspensions at effective antibacterial concentrations	1359:1422	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	9	56	contain	have	1424:1427	arg2	toxicity					1465:1472	in vivo toxicity	1457:1472	in vivo toxicity	1457:1472	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	1	57	theme	bacterial	169:177	arg1	infections					179:188	bacterial infections	169:188	bacterial infections	169:188	Antibiotics are widely used for treatment of bacterial infections, and their overuse has contributed to microbial resistance.					
35007031	3	58	theme	layered	428:434	arg1	material					436:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	a biocompatible and nontoxic rising-star two-dimensional layered material	371:443	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	3	58	theme	layered	428:434	arg1	phosphorus					354:363	Black phosphorus	348:363	Black phosphorus (BP)	348:368	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	9	59	theme	in	1457:1458	arg1	toxicity					1465:1472	in vivo toxicity	1457:1472	in vivo toxicity	1457:1472	Furthermore, the BP-QCS suspensions at effective antibacterial concentrations have negligible cytotoxicity and in vivo toxicity.					
35007031	8	60	theme	BP-QCS	1265:1270	arg1	composite					1272:1280	the BP-QCS composite	1261:1280	the BP-QCS composite under NIR	1261:1290	Meanwhile, the BP-QCS composite under NIR can synergistically inactivate 98% S. aureus in vivo.					
35007031	8	60	theme	BP-QCS	1265:1270	arg1	Meanwhile					1250:1258	Meanwhile	1250:1258	Meanwhile	1250:1258	Meanwhile, the BP-QCS composite under NIR can synergistically inactivate 98% S. aureus in vivo.					
35007031	2	61	theme	antibiotic-free	276:290	arg1	therapy					292:298	an alternative antibiotic-free therapy	261:298	an alternative antibiotic-free therapy for inactivating bacteria	261:324	Currently, an alternative antibiotic-free therapy for inactivating bacteria is of great interest.					
35007031	3	62	theme	antibacterial	563:575	arg1	treatment					577:585	antibacterial treatment	563:585	antibacterial treatment	563:585	Black phosphorus (BP), a biocompatible and nontoxic rising-star two-dimensional layered material, has gained remarkable interest in many bioapplications including biosensing, cancer therapy, drug delivery, and also antibacterial treatment.					
35007031	4	63	theme	rapid	666:670	arg1	oxidation					672:680	rapid oxidation	666:680	rapid oxidation	666:680	However, BP nanosheets suffer from instability in ambient environments due to rapid oxidation and degradation.					
34643089	8	0	located	observed	1315:1322	arg2	trends					1308:1313	trends	1308:1313	trends observed in improvement of metabolic phenotype	1308:1360	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	8	0	located	observed	1315:1322	arg1	improvement					1327:1337	improvement	1327:1337	improvement of metabolic phenotype	1327:1360	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	12	1	theme	acid	2092:2095	arg1	composition					2097:2107	bile acid composition	2087:2107	bile acid composition	2087:2107	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	3	2	theme	gut-derived	509:519	arg1	signals					521:527	these gut-derived signals	503:527	these gut-derived signals	503:527	This study investigated whether dietary forms of Y-BG modulate these gut-derived signals.					
34643089	9	3	theme	insulin	1386:1392	arg1	sensitization					1394:1406	insulin sensitization	1386:1406	insulin sensitization	1386:1406	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	10	4	theme	gut	1568:1570	arg1	composition					1593:1603	gut microbiota community composition	1568:1603	gut microbiota community composition	1568:1603	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	10	5	theme	diet	1759:1762	arg1	&					1781:1781	an aggressive high-fat diet animal model.NEW &	1736:1781	an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1736:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	12	6	theme	functional	2055:2064	arg1	interactions					2066:2077	functional interactions	2055:2077	functional interactions between bile acid composition, gut microbiota, and metabolic phenotype	2055:2148	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	10	7	from	improvement	1698:1708	arg1	phenotype					1723:1731	metabolic phenotype	1713:1731	metabolic phenotype	1713:1731	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	10	7	from	improvement	1698:1708	arg1	&					1781:1781	an aggressive high-fat diet animal model.NEW &	1736:1781	an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1736:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	10	8	theme	aggressive	1739:1748	arg1	diet					1759:1762	an aggressive high-fat diet	1736:1762	an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1736:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	5	9	theme	acid	830:833	arg1	synthesis					835:843	bile acid synthesis	825:843	bile acid synthesis	825:843	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	0	10	theme	acid	81:84	arg1	metabolism					86:95	bile acid metabolism	76:95	bile acid metabolism in healthy and high-fat diet mouse models	76:137	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	13	11	theme	pathways	2372:2379	arg1	modulation					2348:2357	dietary Y-BG modulation	2335:2357	dietary Y-BG modulation of metabolic pathways	2335:2379	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	4	12	theme	dose	678:681	arg1	effects					604:610	the effects	600:610	the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%)	600:695	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	10	13	theme	Y-BG	1823:1826	arg1	supplementation					1828:1842	dietary Y-BG supplementation	1815:1842	dietary Y-BG supplementation	1815:1842	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	1	14	theme	microbiota	195:204	arg1	composition					206:216	altered gut microbiota composition	183:216	altered gut microbiota composition	183:216	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	4	15	theme	soluble	644:650	arg1	Y-BG					668:671	soluble vs. particulate Y-BG	644:671	soluble vs. particulate Y-BG	644:671	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	15	theme	soluble	644:650	arg1	composition					631:641	different fiber composition	615:641	different fiber composition (soluble vs. particulate Y-BG)	615:672	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	10	16	theme	community	1583:1591	arg1	composition					1593:1603	gut microbiota community composition	1568:1603	gut microbiota community composition	1568:1603	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	0	17	theme	mouse	126:130	arg1	models					132:137	healthy and high-fat diet mouse models	100:137	healthy and high-fat diet mouse models	100:137	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	10	18	theme	model.NEW	1771:1779	arg1	&					1781:1781	an aggressive high-fat diet animal model.NEW &	1736:1781	an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1736:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	5	19	theme	mRNA	806:809	arg1	abundance					811:819	elevated liver Cyp7a1 mRNA abundance	784:819	elevated liver Cyp7a1 mRNA abundance	784:819	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	0	20	from	metabolism	86:95	arg1	models					132:137	healthy and high-fat diet mouse models	100:137	healthy and high-fat diet mouse models	100:137	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	11	21	from	obesity	1998:2004	arg1	cohorts					2015:2021	human cohorts	2009:2021	human cohorts	2009:2021	Y-BG significantly reduced Bilophila abundance which is associated with obesity in human cohorts.					
34643089	4	22	theme	particulate	656:666	arg1	Y-BG					668:671	soluble vs. particulate Y-BG	644:671	soluble vs. particulate Y-BG	644:671	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	22	theme	particulate	656:666	arg1	composition					631:641	different fiber composition	615:641	different fiber composition (soluble vs. particulate Y-BG)	615:672	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	0	23	theme	obesity-associated	23:40	arg1	abundance					52:60	obesity-associated Bilophila abundance	23:60	obesity-associated Bilophila abundance	23:60	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	5	24	theme	elevated	784:791	arg1	abundance					811:819	elevated liver Cyp7a1 mRNA abundance	784:819	elevated liver Cyp7a1 mRNA abundance	784:819	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	6	25	theme	prebiotic	921:929	arg1	inulin					931:936	the prebiotic inulin	917:936	the prebiotic inulin	917:936	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	8	26	theme	microbiota	1247:1256	arg1	composition					1258:1268	the gut microbiota composition	1239:1268	the gut microbiota composition	1239:1268	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	10	27	theme	acid	1614:1617	arg1	signaling					1619:1627	gut microbiota community composition and bile acid signaling	1568:1627	gut microbiota community composition and bile acid signaling	1568:1627	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	13	28	theme	Y-BG	2343:2346	arg1	modulation					2348:2357	dietary Y-BG modulation	2335:2357	dietary Y-BG modulation of metabolic pathways	2335:2379	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	2	29	theme	Yeast	275:279	arg1	supplement					310:319	a dietary supplement	300:319	a dietary supplement known for its immunomodulatory effect	300:357	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	2	29	theme	Yeast	275:279	arg1	Y-BG					291:294	Y-BG	291:294	Y-BG	291:294	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	2	29	theme	Yeast	275:279	arg1	β-glucan					281:288	Yeast β-glucan	275:288	Yeast β-glucan (Y-BG)	275:295	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	9	30	theme	Bilophila	1489:1497	arg1	abundance					1499:1507	reduced Bilophila abundance	1481:1507	reduced Bilophila abundance	1481:1507	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	13	31	theme	modulation	2348:2357	arg1	potential					2322:2330	future potential	2315:2330	future potential of dietary Y-BG modulation of metabolic pathways	2315:2379	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	9	32	theme	mRNA	1459:1462	arg1	accumulation					1464:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	8	33	theme	Bilophila	1282:1290	arg1	abundance					1292:1300	reduced Bilophila abundance	1274:1300	reduced Bilophila abundance	1274:1300	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	5	34	theme	particulate	715:725	arg1	Y-BG					727:730	2% particulate Y-BG	712:730	2% particulate Y-BG	712:730	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	5	35	theme	liver	793:797	arg1	abundance					811:819	elevated liver Cyp7a1 mRNA abundance	784:819	elevated liver Cyp7a1 mRNA abundance	784:819	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	13	36	theme	future	2315:2320	arg1	potential					2322:2330	future potential	2315:2330	future potential of dietary Y-BG modulation of metabolic pathways	2315:2379	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	6	37	theme	Bilophila	980:988	arg1	abundance					990:998	fecal Bilophila abundance	974:998	fecal Bilophila abundance	974:998	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	7	38	theme	60	1193:1194	arg1	%					1195:1195	%	1195:1195	%	1195:1195	This prompted us to test whether 2% Y-BG maintained metabolic health in mice fed 60% HFD over 13 wk.					
34643089	10	39	theme	metabolic	1713:1721	arg1	phenotype					1723:1731	metabolic phenotype	1713:1731	metabolic phenotype	1713:1731	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	0	40	theme	healthy	100:106	arg1	models					132:137	healthy and high-fat diet mouse models	100:137	healthy and high-fat diet mouse models	100:137	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	6	41	theme	marked	954:959	arg1	reduction					961:969	a marked reduction	952:969	a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts	952:1109	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	6	42	from	reduction	961:969	arg1	abundance					990:998	fecal Bilophila abundance	974:998	fecal Bilophila abundance	974:998	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	2	43	theme	acid	406:409	arg1	composition					411:421	the gut microbiota and bile acid composition	378:421	composition	411:421	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	5	44	theme	2	712:712	arg1	%					713:713	%	713:713	%	713:713	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	6	45	theme	population-scale	1052:1067	arg1	Gut					1078:1080	population-scale American Gut and TwinsUK clinical cohorts	1052:1109	Gut	1078:1080	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	1	46	theme	Emerging	140:147	arg1	evidence					149:156	Emerging evidence	140:156	Emerging evidence	140:156	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	7	47	theme	%	1195:1195	arg1	HFD					1197:1199	60% HFD	1193:1199	60% HFD	1193:1199	This prompted us to test whether 2% Y-BG maintained metabolic health in mice fed 60% HFD over 13 wk.					
34643089	10	48	theme	dietary	1638:1644	arg1	regime					1646:1651	the dietary regime	1634:1651	the dietary regime	1634:1651	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	0	49	theme	Yeast	0:4	arg1	β-glucan					6:13	Yeast β-glucan	0:13	Yeast β-glucan	0:13	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	8	50	theme	phenotype	1352:1360	arg1	improvement					1327:1337	improvement	1327:1337	improvement of metabolic phenotype	1327:1360	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	4	51	from	supplementation	556:570	arg1	mice					583:586	healthy mice	575:586	healthy mice	575:586	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	52	theme	healthy	575:581	arg1	mice					583:586	healthy mice	575:586	healthy mice	575:586	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	10	53	theme	bile	1870:1873	arg1	metabolism					1880:1889	bile acid metabolism	1870:1889	bile acid metabolism	1870:1889	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	2	54	theme	bile	401:404	arg1	composition					411:421	the gut microbiota and bile acid composition	378:421	composition	411:421	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	0	55	theme	Bilophila	42:50	arg1	abundance					52:60	obesity-associated Bilophila abundance	23:60	obesity-associated Bilophila abundance	23:60	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	5	56	theme	gut	747:749	arg1	shifts					772:777	robust gut microbiota community shifts	740:777	robust gut microbiota community shifts	740:777	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	0	57	theme	bile	76:79	arg1	metabolism					86:95	bile acid metabolism	76:95	bile acid metabolism in healthy and high-fat diet mouse models	76:137	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	3	58	theme	Y-BG	489:492	arg1	forms					480:484	dietary forms	472:484	dietary forms of Y-BG	472:492	This study investigated whether dietary forms of Y-BG modulate these gut-derived signals.					
34643089	5	59	theme	community	762:770	arg1	shifts					772:777	robust gut microbiota community shifts	740:777	robust gut microbiota community shifts	740:777	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	4	60	theme	4-wk	543:546	arg1	supplementation					556:570	4-wk dietary supplementation	543:570	4-wk dietary supplementation in healthy mice	543:586	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	10	61	theme	associated	1895:1904	arg1	pathways					1916:1923	associated signaling pathways	1895:1923	associated signaling pathways	1895:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	10	62	theme	clinical	1689:1696	arg1	improvement					1698:1708	clinical improvement	1689:1708	clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1689:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	11	63	theme	Bilophila	1953:1961	arg1	abundance					1963:1971	Bilophila abundance	1953:1971	Bilophila abundance which is associated with obesity in human cohorts	1953:2021	Y-BG significantly reduced Bilophila abundance which is associated with obesity in human cohorts.					
34643089	12	64	theme	obesity	2221:2227	arg1	model					2229:2233	an aggressive obesity model	2207:2233	an aggressive obesity model	2207:2233	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	1	65	theme	altered	183:189	arg1	composition					206:216	altered gut microbiota composition	183:216	altered gut microbiota composition	183:216	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	13	66	theme	Gut	2236:2238	arg1	microbiota					2240:2249	Gut microbiota	2236:2249	Gut microbiota	2236:2249	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	7	67	theme	metabolic	1164:1172	arg1	health					1174:1179	metabolic health	1164:1179	metabolic health	1164:1179	This prompted us to test whether 2% Y-BG maintained metabolic health in mice fed 60% HFD over 13 wk.					
34643089	6	68	theme	TwinsUK	1086:1092	arg1	cohorts					1103:1109	population-scale American Gut and TwinsUK clinical cohorts	1052:1109	cohorts	1103:1109	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	4	69	theme	fiber	625:629	arg1	Y-BG					668:671	soluble vs. particulate Y-BG	644:671	soluble vs. particulate Y-BG	644:671	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	69	theme	fiber	625:629	arg1	composition					631:641	different fiber composition	615:641	different fiber composition (soluble vs. particulate Y-BG)	615:672	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	12	70	theme	bile	2087:2090	arg1	composition					2097:2107	bile acid composition	2087:2107	bile acid composition	2087:2107	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	10	71	theme	microbiota	1572:1581	arg1	composition					1593:1603	gut microbiota community composition	1568:1603	gut microbiota community composition	1568:1603	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	10	72	theme	animal	1764:1769	arg1	&					1781:1781	an aggressive high-fat diet animal model.NEW &	1736:1781	an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1736:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	12	73	theme	Correlation	2024:2034	arg1	analysis					2036:2043	Correlation analysis	2024:2043	Correlation analysis	2024:2043	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	10	74	theme	high-fat	1750:1757	arg1	diet					1759:1762	an aggressive high-fat diet	1736:1762	an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways	1736:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	5	75	theme	bile	825:828	arg1	synthesis					835:843	bile acid synthesis	825:843	bile acid synthesis	825:843	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	11	76	theme	human	2009:2013	arg1	cohorts					2015:2021	human cohorts	2009:2021	human cohorts	2009:2021	Y-BG significantly reduced Bilophila abundance which is associated with obesity in human cohorts.					
34643089	6	77	theme	diet-induced	852:863	arg1	responses					865:873	These diet-induced responses	846:873	These diet-induced responses	846:873	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	10	78	theme	bile	1609:1612	arg1	acid					1614:1617	bile acid	1609:1617	bile acid	1609:1617	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	1	79	theme	gut	191:193	arg1	composition					206:216	altered gut microbiota composition	183:216	altered gut microbiota composition	183:216	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	2	80	from	impact	368:373	arg1	microbiota					386:395	the gut microbiota and bile acid composition	378:421	microbiota	386:395	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	2	80	from	impact	368:373	arg1	composition					411:421	the gut microbiota and bile acid composition	378:421	composition	411:421	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	10	81	theme	composition	1593:1603	arg1	signaling					1619:1627	gut microbiota community composition and bile acid signaling	1568:1627	gut microbiota community composition and bile acid signaling	1568:1627	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	6	82	from	translatable	1025:1036	arg1	Gut					1078:1080	population-scale American Gut and TwinsUK clinical cohorts	1052:1109	Gut	1078:1080	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	6	82	from	translatable	1025:1036	arg1	cohorts					1103:1109	population-scale American Gut and TwinsUK clinical cohorts	1052:1109	cohorts	1103:1109	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	2	83	theme	dietary	302:308	arg1	supplement					310:319	a dietary supplement	300:319	a dietary supplement known for its immunomodulatory effect	300:357	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	2	83	theme	dietary	302:308	arg1	β-glucan					281:288	Yeast β-glucan	275:288	Yeast β-glucan (Y-BG)	275:295	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	9	84	theme	enhanced	1437:1444	arg1	accumulation					1464:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	3	85	link	gut-derived	509:519	arg1	signals					521:527	these gut-derived signals	503:527	these gut-derived signals	503:527	This study investigated whether dietary forms of Y-BG modulate these gut-derived signals.					
34643089	10	86	theme	dietary	1815:1821	arg1	supplementation					1828:1842	dietary Y-BG supplementation	1815:1842	dietary Y-BG supplementation	1815:1842	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	1	87	theme	bile	222:225	arg1	acid					227:230	bile acid	222:230	bile acid signaling	222:240	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	12	88	theme	clinical	2160:2167	arg1	benefit					2169:2175	clinical benefit	2160:2175	clinical benefit	2160:2175	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	13	89	theme	dietary	2335:2341	arg1	modulation					2348:2357	dietary Y-BG modulation	2335:2357	dietary Y-BG modulation of metabolic pathways	2335:2379	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	8	90	theme	gut	1243:1245	arg1	composition					1258:1268	the gut microbiota composition	1239:1268	the gut microbiota composition	1239:1268	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	10	91	theme	gut	1854:1856	arg1	microbiota					1858:1867	gut microbiota	1854:1867	gut microbiota	1854:1867	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	9	92	theme	reduced	1481:1487	arg1	abundance					1499:1507	reduced Bilophila abundance	1481:1507	reduced Bilophila abundance	1481:1507	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	12	93	theme	metabolic	2130:2138	arg1	phenotype					2140:2148	metabolic phenotype	2130:2148	metabolic phenotype	2130:2148	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	5	94	theme	%	713:713	arg1	Y-BG					727:730	2% particulate Y-BG	712:730	2% particulate Y-BG	712:730	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	5	95	theme	Cyp7a1	799:804	arg1	abundance					811:819	elevated liver Cyp7a1 mRNA abundance	784:819	elevated liver Cyp7a1 mRNA abundance	784:819	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	8	96	theme	reduced	1274:1280	arg1	abundance					1292:1300	reduced Bilophila abundance	1274:1300	reduced Bilophila abundance	1274:1300	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	1	97	theme	dietary	164:170	arg1	fiber					172:176	dietary fiber	164:176	dietary fiber	164:176	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	6	98	theme	fecal	974:978	arg1	abundance					990:998	fecal Bilophila abundance	974:998	fecal Bilophila abundance	974:998	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	12	99	theme	gut	2110:2112	arg1	microbiota					2114:2123	gut microbiota	2110:2123	gut microbiota	2110:2123	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	13	100	theme	metabolic	2362:2370	arg1	pathways					2372:2379	metabolic pathways	2362:2379	metabolic pathways	2362:2379	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	0	101	theme	diet	121:124	arg1	models					132:137	healthy and high-fat diet mouse models	100:137	healthy and high-fat diet mouse models	100:137	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	9	102	theme	ileal	1446:1450	arg1	accumulation					1464:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	6	103	theme	American	1069:1076	arg1	Gut					1078:1080	population-scale American Gut and TwinsUK clinical cohorts	1052:1109	Gut	1078:1080	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	2	104	theme	gut	382:384	arg1	microbiota					386:395	the gut microbiota and bile acid composition	378:421	microbiota	386:395	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	7	105	theme	2	1145:1145	arg1	%					1146:1146	%	1146:1146	%	1146:1146	This prompted us to test whether 2% Y-BG maintained metabolic health in mice fed 60% HFD over 13 wk.					
34643089	13	106	theme	metabolic	2282:2290	arg1	parameters					2292:2301	metabolic parameters	2282:2301	metabolic parameters	2282:2301	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	10	107	theme	acid	1875:1878	arg1	metabolism					1880:1889	bile acid metabolism	1870:1889	bile acid metabolism	1870:1889	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	8	108	theme	metabolic	1342:1350	arg1	phenotype					1352:1360	metabolic phenotype	1342:1360	metabolic phenotype	1342:1360	Y-BG consistently altered the gut microbiota composition and reduced Bilophila abundance, with trends observed in improvement of metabolic phenotype.					
34643089	3	109	theme	dietary	472:478	arg1	forms					480:484	dietary forms	472:484	dietary forms of Y-BG	472:492	This study investigated whether dietary forms of Y-BG modulate these gut-derived signals.					
34643089	5	110	theme	robust	740:745	arg1	shifts					772:777	robust gut microbiota community shifts	740:777	robust gut microbiota community shifts	740:777	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	9	111	theme	Glpr1r	1452:1457	arg1	accumulation					1464:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	enhanced ileal Glpr1r mRNA accumulation	1437:1475	Notably, Y-BG improved insulin sensitization and this was associated with enhanced ileal Glpr1r mRNA accumulation and reduced Bilophila abundance.					
34643089	0	112	theme	high-fat	112:119	arg1	diet					121:124	high-fat diet	112:124	high-fat diet	112:124	Yeast β-glucan reduces obesity-associated Bilophila abundance and modulates bile acid metabolism in healthy and high-fat diet mouse models.					
34643089	5	113	theme	microbiota	751:760	arg1	shifts					772:777	robust gut microbiota community shifts	740:777	robust gut microbiota community shifts	740:777	We found that 2% particulate Y-BG induced robust gut microbiota community shifts with elevated liver Cyp7a1 mRNA abundance and bile acid synthesis.					
34643089	13	114	theme	bile	2255:2258	arg1	acids					2260:2264	bile acids	2255:2264	bile acids	2255:2264	Gut microbiota and bile acids correlated with metabolic parameters, indicating future potential of dietary Y-BG modulation of metabolic pathways.					
34643089	4	115	theme	dietary	548:554	arg1	supplementation					556:570	4-wk dietary supplementation	543:570	4-wk dietary supplementation in healthy mice	543:586	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	10	116	theme	signaling	1906:1914	arg1	pathways					1916:1923	associated signaling pathways	1895:1923	associated signaling pathways	1895:1923	Collectively, our results demonstrate that Y-BG modulates gut microbiota community composition and bile acid signaling, but the dietary regime needs to be optimized to facilitate clinical improvement in metabolic phenotype in an aggressive high-fat diet animal model.NEW & NOTEWORTHY The study shows that dietary Y-BG supplementation modulated gut microbiota, bile acid metabolism and associated signaling pathways.					
34643089	2	117	theme	immunomodulatory	335:350	arg1	effect					352:357	its immunomodulatory effect	331:357	its immunomodulatory effect	331:357	Yeast β-glucan (Y-BG) is a dietary supplement known for its immunomodulatory effect, yet its impact on the gut microbiota and bile acid composition remains unclear.					
34643089	4	118	dep	dose	678:681	arg1	%					687:687	0.1% vs. 2%	684:694	%	687:687	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	118	dep	dose	678:681	arg1	%					694:694	0.1% vs. 2%	684:694	%	694:694	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	119	theme	different	615:623	arg1	Y-BG					668:671	soluble vs. particulate Y-BG	644:671	soluble vs. particulate Y-BG	644:671	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	4	119	theme	different	615:623	arg1	composition					631:641	different fiber composition	615:641	different fiber composition (soluble vs. particulate Y-BG)	615:672	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	1	120	theme	acid	227:230	arg1	signaling					232:240	bile acid signaling	222:240	bile acid signaling	222:240	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
34643089	6	121	theme	clinical	1094:1101	arg1	cohorts					1103:1109	population-scale American Gut and TwinsUK clinical cohorts	1052:1109	cohorts	1103:1109	These diet-induced responses were notably different when compared with the prebiotic inulin, and included a marked reduction in fecal Bilophila abundance which we demonstrated as translatable to obesity in population-scale American Gut and TwinsUK clinical cohorts.					
34643089	4	122	theme	composition	631:641	arg1	effects					604:610	the effects	600:610	the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%)	600:695	We performed 4-wk dietary supplementation in healthy mice to evaluate the effects of different fiber composition (soluble vs. particulate Y-BG) and dose (0.1% vs. 2%).					
34643089	12	123	theme	aggressive	2210:2219	arg1	model					2229:2233	an aggressive obesity model	2207:2233	an aggressive obesity model	2207:2233	Correlation analysis confirmed functional interactions between bile acid composition, gut microbiota, and metabolic phenotype, although clinical benefit did not reach significance in an aggressive obesity model.					
34643089	7	124	theme	%	1146:1146	arg1	Y-BG					1148:1151	2% Y-BG	1145:1151	2% Y-BG	1145:1151	This prompted us to test whether 2% Y-BG maintained metabolic health in mice fed 60% HFD over 13 wk.					
34643089	1	125	theme	metabolic	257:265	arg1	health					267:272	metabolic health	257:272	metabolic health	257:272	Emerging evidence links dietary fiber with altered gut microbiota composition and bile acid signaling in maintaining metabolic health.					
32585176	0	0	theme	inkjet	84:89	arg1	printing					91:98	pharmaceutical inkjet printing	69:98	pharmaceutical inkjet printing	69:98	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	4	1	theme	piezoelectric	482:494	arg1	heads					502:506	two piezoelectric print heads	478:506	two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX)	478:575	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	8	2	dep	size	1296:1299	arg1	the					1292:1294	the	1292:1294	the	1292:1294	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	4	3	from	factors	688:694	arg1	quantities					712:721	the resulted quantities	699:721	the resulted quantities	699:721	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	0	4	theme	pharmaceutical	69:82	arg1	printing					91:98	pharmaceutical inkjet printing	69:98	pharmaceutical inkjet printing	69:98	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	0	5	from	investigations	12:25	arg1	designs					57:63	print head designs	46:63	print head designs	46:63	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	0	5	from	investigations	12:25	arg1	factors					34:40	key factors	30:40	key factors	30:40	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	7	6	theme	limited	1060:1066	arg1	penetration					1068:1078	limited penetration	1060:1078	limited penetration into the HPMC based films	1060:1104	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	8	7	theme	printed	1142:1148	arg1	geometry					1150:1157	the printed geometry	1138:1157	the printed geometry	1138:1157	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	7	8	theme	liquid	972:977	arg1	excipients					979:988	liquid excipients	972:988	liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films	972:1104	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	7	9	theme	vapour	1040:1045	arg1	pressure					1047:1054	low vapour pressure	1036:1054	low vapour pressure	1036:1054	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	3	10	theme	comparative	376:386	arg1	investigations					388:401	comparative investigations	376:401	comparative investigations in relation to pharmaceutical use	376:435	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	8	11	dep	plays	1159:1163	arg1	whereas					1221:1227	whereas	1221:1227	whereas	1221:1227	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	6	12	theme	bigger	851:856	arg1	nozzles					858:864	bigger nozzles	851:864	bigger nozzles	851:864	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
32585176	8	13	theme	inks	1252:1255	arg1	composition					1233:1243	the composition	1229:1243	the composition of the inks, set process parameters as well as the size and functionality of the nozzles	1229:1332	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	13	theme	inks	1252:1255	arg1	functionality					1305:1317	functionality	1305:1317	functionality	1305:1317	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	13	theme	inks	1252:1255	arg1	size					1296:1299	size	1296:1299	size	1296:1299	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	4	14	theme	print	496:500	arg1	heads					502:506	two piezoelectric print heads	478:506	two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX)	478:575	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	5	15	theme	casting	795:801	arg1	solution					803:810	hypromellose (HPMC) casting solution	775:810	15% (w/w) hypromellose (HPMC) casting solution	765:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	2	16	theme	printer	271:277	arg1	head					234:237	The print head	224:237	The print head	224:237	The print head is the centrepiece of an inkjet printer.					
32585176	2	16	theme	printer	271:277	arg1	centrepiece					246:256	the centrepiece	242:256	the centrepiece of an inkjet printer	242:277	The print head is the centrepiece of an inkjet printer.					
32585176	6	17	with	Spectra	827:833	arg1	nozzles					858:864	bigger nozzles	851:864	bigger nozzles	851:864	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
32585176	1	18	theme	personalized	171:182	arg1	medicine					184:191	personalized medicine	171:191	personalized medicine	171:191	The interest in using inkjet printing as manufacturing technology for personalized medicine has increased in recent years.					
32585176	5	19	theme	oral	739:742	arg1	films					749:753	oral thin films	739:753	oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution	739:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	5	19	theme	oral	739:742	arg1	substrates					727:736	substrates	727:736	substrates	727:736	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	7	20	theme	low	1036:1038	arg1	pressure					1047:1054	low vapour pressure	1036:1054	low vapour pressure	1036:1054	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	2	21	theme	inkjet	264:269	arg1	printer					271:277	an inkjet printer	261:277	an inkjet printer	261:277	The print head is the centrepiece of an inkjet printer.					
32585176	4	22	theme	process	642:648	arg1	understanding					650:662	the process understanding	638:662	the process understanding	638:662	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	6	23	theme	print	835:839	arg1	head					841:844	print head	835:844	The Spectra print head with bigger nozzles	823:864	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
32585176	5	24	theme	thin	744:747	arg1	films					749:753	oral thin films	739:753	oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution	739:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	5	24	theme	thin	744:747	arg1	substrates					727:736	substrates	727:736	substrates	727:736	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	4	25	theme	present	463:469	arg1	study					471:475	the present study	459:475	the present study	459:475	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	3	26	theme	pharmaceutical	418:431	arg1	use					433:435	pharmaceutical use	418:435	pharmaceutical use	418:435	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	0	27	theme	Comparative	0:10	arg1	investigations					12:25	Comparative investigations	0:25	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing	0:98	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	5	28	dep	%	767:767	arg1	solution					803:810	hypromellose (HPMC) casting solution	775:810	15% (w/w) hypromellose (HPMC) casting solution	765:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	8	29	theme	subordinate	1167:1177	arg1	role					1179:1182	a subordinate role	1165:1182	a subordinate role	1165:1182	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	0	30	theme	key	30:32	arg1	factors					34:40	key factors	30:40	key factors	30:40	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	5	31	theme	hypromellose	775:786	arg1	solution					803:810	hypromellose (HPMC) casting solution	775:810	15% (w/w) hypromellose (HPMC) casting solution	765:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	7	32	theme	polyethylene	995:1006	arg1	glycol					1008:1013	polyethylene glycol 400	995:1017	polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films	995:1104	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	5	33	theme	HPMC	789:792	arg1	solution					803:810	hypromellose (HPMC) casting solution	775:810	15% (w/w) hypromellose (HPMC) casting solution	765:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	8	34	theme	process	1262:1268	arg1	inks					1252:1255	the inks	1248:1255	the inks	1248:1255	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	34	theme	process	1262:1268	arg1	parameters					1270:1279	set process parameters	1258:1279	set process parameters	1258:1279	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	35	theme	significant	1341:1351	arg1	impact					1353:1358	a significant impact	1339:1358	a significant impact	1339:1358	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	5	36	used	used	817:820	arg2	films					749:753	oral thin films	739:753	oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution	739:810	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	5	36	used	used	817:820	arg2	substrates					727:736	substrates	727:736	substrates	727:736	As substrates, oral thin films made from 15% (w/w) hypromellose (HPMC) casting solution were used.					
32585176	1	37	theme	recent	210:215	arg1	years					217:221	recent years	210:221	recent years	210:221	The interest in using inkjet printing as manufacturing technology for personalized medicine has increased in recent years.					
32585176	8	38	theme	set	1258:1260	arg1	inks					1252:1255	the inks	1248:1255	the inks	1248:1255	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	38	theme	set	1258:1260	arg1	parameters					1270:1279	set process parameters	1258:1279	set process parameters	1258:1279	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	39	theme	same	1202:1205	arg1	area					1215:1218	the same surface area	1198:1218	the same surface area	1198:1218	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	7	40	theme	HPMC	1089:1092	arg1	films					1100:1104	the HPMC based films	1085:1104	the HPMC based films	1085:1104	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	4	41	theme	designs	521:527	arg1	heads					502:506	two piezoelectric print heads	478:506	two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX)	478:575	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	4	42	dep	deepening	628:636	arg1	objective					615:623	objective	615:623	objective	615:623	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	4	42	dep	deepening	628:636	arg1	the					611:613	the	611:613	the	611:613	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	0	43	theme	head	52:55	arg1	designs					57:63	print head designs	46:63	print head designs	46:63	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	4	44	theme	different	511:519	arg1	Spectra					530:536	Spectra SE-128 AA and Konica Minolta KM512SHX	530:574	Spectra SE-128 AA and Konica Minolta KM512SHX	530:574	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	4	44	theme	different	511:519	arg1	designs					521:527	different designs	511:527	different designs (Spectra SE-128 AA and Konica Minolta KM512SHX)	511:575	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	2	45	theme	print	228:232	arg1	head					234:237	The print head	224:237	The print head	224:237	The print head is the centrepiece of an inkjet printer.					
32585176	2	45	theme	print	228:232	arg1	centrepiece					246:256	the centrepiece	242:256	the centrepiece of an inkjet printer	242:277	The print head is the centrepiece of an inkjet printer.					
32585176	4	46	theme	resulted	703:710	arg1	quantities					712:721	the resulted quantities	699:721	the resulted quantities	699:721	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	0	47	theme	print	46:50	arg1	designs					57:63	print head designs	46:63	print head designs	46:63	Comparative investigations on key factors and print head designs for pharmaceutical inkjet printing.					
32585176	6	48	dep	Spectra	827:833	arg1	head					841:844	print head	835:844	The Spectra print head with bigger nozzles	823:864	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
32585176	3	49	theme	various	311:317	arg1	types					319:323	various types	311:323	various types of printing equipment	311:345	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	8	50	theme	final	1367:1371	arg1	quantity					1381:1388	the final printed quantity	1363:1388	the final printed quantity	1363:1388	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	51	theme	surface	1207:1213	arg1	area					1215:1218	the same surface area	1198:1218	the same surface area	1198:1218	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	7	52	theme	based	1094:1098	arg1	films					1100:1104	the HPMC based films	1085:1104	the HPMC based films	1085:1104	Furthermore, it was found that liquid excipients like polyethylene glycol 400 characterized by low vapour pressure and limited penetration into the HPMC based films are not suitable.					
32585176	3	53	theme	pharmaceutical	284:297	arg1	approaches					299:308	pharmaceutical approaches	284:308	pharmaceutical approaches	284:308	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	4	54	dep	Spectra	530:536	arg1	AA					545:546	SE-128 AA	538:546	SE-128 AA	538:546	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	4	54	dep	Spectra	530:536	arg1	Spectra					530:536	Spectra SE-128 AA and Konica Minolta KM512SHX	530:574	Spectra SE-128 AA and Konica Minolta KM512SHX	530:574	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	4	54	dep	Spectra	530:536	arg1	KM512SHX					567:574	KM512SHX	567:574	KM512SHX	567:574	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	1	55	theme	inkjet	123:128	arg1	printing					130:137	inkjet printing	123:137	inkjet printing	123:137	The interest in using inkjet printing as manufacturing technology for personalized medicine has increased in recent years.					
32585176	6	56	from	pass	892:895	arg1	Spectra					827:833	The Spectra print head	823:844	The Spectra print head with bigger nozzles	823:864	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
32585176	6	56	from	pass	892:895	arg1	efficient					875:883	efficient	875:883	efficient	875:883	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
32585176	8	57	theme	geometry	1150:1157	arg1	choice					1128:1133	The choice	1124:1133	The choice of the printed geometry	1124:1157	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	3	58	theme	printing	328:335	arg1	equipment					337:345	printing equipment	328:345	printing equipment	328:345	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	8	59	theme	printed	1373:1379	arg1	quantity					1381:1388	the final printed quantity	1363:1388	the final printed quantity	1363:1388	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	60	contain	have	1334:1337	arg1	composition					1233:1243	the composition	1229:1243	the composition of the inks, set process parameters as well as the size and functionality of the nozzles	1229:1332	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	60	contain	have	1334:1337	arg1	size					1296:1299	size	1296:1299	size	1296:1299	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	60	contain	have	1334:1337	arg2	impact					1353:1358	a significant impact	1339:1358	a significant impact	1339:1358	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	4	61	theme	key	684:686	arg1	factors					688:694	the key factors	680:694	the key factors on the resulted quantities	680:721	In the present study, two piezoelectric print heads of different designs (Spectra SE-128 AA and Konica Minolta KM512SHX) were systematically compared with the objective of deepening the process understanding and identifying the key factors on the resulted quantities.					
32585176	3	62	theme	equipment	337:345	arg1	types					319:323	various types	311:323	various types of printing equipment	311:345	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	3	63	from	investigations	388:401	arg1	relation					406:413	relation	406:413	relation to pharmaceutical use	406:435	For pharmaceutical approaches, various types of printing equipment were tested in the past, but comparative investigations in relation to pharmaceutical use are still lacking.					
32585176	8	64	theme	nozzles	1326:1332	arg1	composition					1233:1243	the composition	1229:1243	the composition of the inks, set process parameters as well as the size and functionality of the nozzles	1229:1332	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	64	theme	nozzles	1326:1332	arg1	functionality					1305:1317	functionality	1305:1317	functionality	1305:1317	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	8	64	theme	nozzles	1326:1332	arg1	size					1296:1299	size	1296:1299	size	1296:1299	The choice of the printed geometry plays a subordinate role when printing the same surface area, whereas the composition of the inks, set process parameters as well as the size and functionality of the nozzles have a significant impact on the final printed quantity.					
32585176	1	65	theme	manufacturing	142:154	arg1	technology					156:165	manufacturing technology	142:165	manufacturing technology for personalized medicine	142:191	The interest in using inkjet printing as manufacturing technology for personalized medicine has increased in recent years.					
32585176	6	66	from	efficient	875:883	arg1	pass					892:895	one pass	888:895	one pass	888:895	The Spectra print head with bigger nozzles was more efficient in one pass and resulted in less scattering (RSD ≤ 5%).					
33321345	4	0	theme	digestible	628:637	arg1	starch					639:644	slowly digestible starch	621:644	slowly digestible starch	621:644	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	1	1	theme	leavened	142:149	arg1	food					158:161	a leavened staple food	140:161	a leavened staple food among the native highlanders of Western Himalaya	140:210	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	1	1	theme	leavened	142:149	arg1	Khambir					129:135	Khambir	129:135	Khambir	129:135	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	0	2	theme	potentialities	79:92	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir	0:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	2	theme	potentialities	79:92	arg1	bread					122:126	a leavened flat bread	106:126	a leavened flat bread	106:126	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	7	3	theme	traditional	1056:1066	arg1	nutritious					1094:1103	nutritious	1094:1103	nutritious	1094:1103	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	7	3	theme	traditional	1056:1066	arg1	khambir					1083:1089	the traditional leavened bread khambir	1052:1089	the traditional leavened bread khambir	1052:1089	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	7	4	theme	bread	1077:1081	arg1	nutritious					1094:1103	nutritious	1094:1103	nutritious	1094:1103	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	7	4	theme	bread	1077:1081	arg1	khambir					1083:1089	the traditional leavened bread khambir	1052:1089	the traditional leavened bread khambir	1052:1089	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	1	5	theme	staple	151:156	arg1	food					158:161	a leavened staple food	140:161	a leavened staple food among the native highlanders of Western Himalaya	140:210	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	1	5	theme	staple	151:156	arg1	Khambir					129:135	Khambir	129:135	Khambir	129:135	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	6	6	theme	gastric	922:928	arg1	ulcers					930:935	cold-induced gastric ulcers	909:935	cold-induced gastric ulcers	909:935	The extracts of khambir alleviated cold-induced gastric ulcers in the animal model as it exhibited histoprotective and anti-inflammatory activities.					
33321345	7	7	theme	acute	1150:1154	arg1	sickness					1165:1172	acute mountain sickness	1150:1172	acute mountain sickness	1150:1172	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	4	8	theme	slow	506:509	arg1	potential					538:546	a slow glucose-controlled release potential	504:546	a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	504:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	0	9	theme	khambir	97:103	arg1	potentialities					79:92	anti-gastric ulcer potentialities	60:92	anti-gastric ulcer potentialities of khambir	60:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	9	theme	khambir	97:103	arg1	profile					23:29	nutrient profile	14:29	nutrient profile	14:29	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	9	theme	khambir	97:103	arg1	composition					44:54	biochemical composition	32:54	biochemical composition	32:54	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	4	10	theme	index	688:692	arg1	content					583:589	improved content	574:589	improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	574:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	3	11	theme	lactic	428:433	arg1	minerals					455:462	minerals	455:462	minerals	455:462	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	11	theme	lactic	428:433	arg1	acid					435:438	lactic acid	428:438	lactic acid	428:438	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	11	theme	lactic	428:433	arg1	vitamins					441:448	vitamins	441:448	vitamins	441:448	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	5	12	theme	protein	770:776	arg1	content					759:765	content	759:765	content of protein and fatty acid	759:791	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	12	theme	protein	770:776	arg1	starch					751:756	starch	751:756	starch	751:756	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	12	theme	protein	770:776	arg1	accumulation					798:809	accumulation	798:809	accumulation of 17 major metabolites	798:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	0	13	theme	leavened	108:115	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir	0:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	13	theme	leavened	108:115	arg1	bread					122:126	a leavened flat bread	106:126	a leavened flat bread	106:126	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	2	14	theme	flour	263:267	arg1	fermentation					241:252	sourdough fermentation	231:252	sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK)	231:304	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	4	15	theme	digestible	602:611	arg1	starch					613:618	rapidly digestible starch	594:618	rapidly digestible starch	594:618	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	5	16	theme	fatty	782:786	arg1	acid					788:791	fatty acid	782:791	fatty acid	782:791	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	17	theme	amorphous	727:735	arg1	structures					737:746	amorphous structures	727:746	amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites	727:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	3	18	from	rich	332:335	arg1	protein					354:360	protein	354:360	protein	354:360	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	18	from	rich	332:335	arg1	fiber					371:375	dietary fiber	363:375	dietary fiber	363:375	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	18	from	rich	332:335	arg1	carbohydrate					340:351	carbohydrate	340:351	carbohydrate	340:351	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	2	19	theme	wheat	257:261	arg1	flour					263:267	wheat flour	257:267	wheat flour	257:267	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	5	20	theme	acid	788:791	arg1	content					759:765	content	759:765	content of protein and fatty acid	759:791	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	20	theme	acid	788:791	arg1	starch					751:756	starch	751:756	starch	751:756	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	20	theme	acid	788:791	arg1	accumulation					798:809	accumulation	798:809	accumulation of 17 major metabolites	798:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	7	21	theme	gastric	1123:1129	arg1	lesions					1131:1137	gastric lesions	1123:1137	gastric lesions related to acute mountain sickness	1123:1172	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	5	22	theme	major	817:821	arg1	metabolites					823:833	17 major metabolites	814:833	17 major metabolites	814:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	3	23	with	enriched	414:421	arg1	minerals					455:462	minerals	455:462	minerals	455:462	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	23	with	enriched	414:421	arg1	acid					435:438	lactic acid	428:438	lactic acid	428:438	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	23	with	enriched	414:421	arg1	vitamins					441:448	vitamins	441:448	vitamins	441:448	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	6	24	theme	khambir	890:896	arg1	extracts					878:885	The extracts	874:885	The extracts of khambir	874:896	The extracts of khambir alleviated cold-induced gastric ulcers in the animal model as it exhibited histoprotective and anti-inflammatory activities.					
33321345	6	25	theme	anti-inflammatory	993:1009	arg1	activities					1011:1020	histoprotective and anti-inflammatory activities	973:1020	histoprotective and anti-inflammatory activities	973:1020	The extracts of khambir alleviated cold-induced gastric ulcers in the animal model as it exhibited histoprotective and anti-inflammatory activities.					
33321345	3	26	theme	dietary	363:369	arg1	fiber					371:375	dietary fiber	363:375	dietary fiber	363:375	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	3	26	theme	dietary	363:369	arg1	carbohydrate					340:351	carbohydrate	340:351	carbohydrate	340:351	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	4	27	theme	in	469:470	arg1	test					492:495	The in vitro digestibility test	465:495	The in vitro digestibility test	465:495	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	1	28	theme	native	173:178	arg1	highlanders					180:190	the native highlanders	169:190	the native highlanders of Western Himalaya	169:210	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	3	29	theme	bread	321:325	arg1	types					312:316	Both types	307:316	Both types of bread	307:325	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	7	30	theme	leavened	1068:1075	arg1	nutritious					1094:1103	nutritious	1094:1103	nutritious	1094:1103	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	7	30	theme	leavened	1068:1075	arg1	khambir					1083:1089	the traditional leavened bread khambir	1052:1089	the traditional leavened bread khambir	1052:1089	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	4	31	theme	glycemic	679:686	arg1	index					688:692	predicted glycemic index	669:692	predicted glycemic index	669:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	5	32	theme	content	759:765	arg1	structures					737:746	amorphous structures	727:746	amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites	727:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	0	33	theme	nutrient	14:21	arg1	profile					23:29	nutrient profile	14:29	nutrient profile	14:29	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	4	34	theme	digestibility	478:490	arg1	test					492:495	The in vitro digestibility test	465:495	The in vitro digestibility test	465:495	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	0	35	theme	flat	117:120	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir	0:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	35	theme	flat	117:120	arg1	bread					122:126	a leavened flat bread	106:126	a leavened flat bread	106:126	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	4	36	theme	predicted	669:677	arg1	index					688:692	predicted glycemic index	669:692	predicted glycemic index	669:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	4	37	theme	khambir	551:557	arg1	potential					538:546	a slow glucose-controlled release potential	504:546	a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	504:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	0	38	theme	biochemical	32:42	arg1	composition					44:54	biochemical composition	32:54	biochemical composition	32:54	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	7	39	theme	related	1139:1145	arg1	lesions					1131:1137	gastric lesions	1123:1137	gastric lesions related to acute mountain sickness	1123:1172	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33321345	4	40	theme	improved	574:581	arg1	content					583:589	improved content	574:589	improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	574:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	1	41	theme	Western	195:201	arg1	Himalaya					203:210	Western Himalaya	195:210	Western Himalaya	195:210	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	5	42	theme	crystallinity	710:722	arg1	changes					699:705	The changes	695:705	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites	695:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	0	43	theme	profile	23:29	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir	0:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	43	theme	profile	23:29	arg1	bread					122:126	a leavened flat bread	106:126	a leavened flat bread	106:126	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	1	44	theme	Himalaya	203:210	arg1	highlanders					180:190	the native highlanders	169:190	the native highlanders of Western Himalaya	169:210	Khambir is a leavened staple food among the native highlanders of Western Himalaya.					
33321345	4	45	dep	in	469:470	arg1	vitro					472:476	vitro	472:476	vitro	472:476	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	5	46	theme	metabolites	823:833	arg1	content					759:765	content	759:765	content of protein and fatty acid	759:791	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	46	theme	metabolites	823:833	arg1	starch					751:756	starch	751:756	starch	751:756	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	5	46	theme	metabolites	823:833	arg1	accumulation					798:809	accumulation	798:809	accumulation of 17 major metabolites	798:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	0	47	theme	composition	44:54	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir	0:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	0	47	theme	composition	44:54	arg1	bread					122:126	a leavened flat bread	106:126	a leavened flat bread	106:126	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	5	48	theme	starch	751:756	arg1	structures					737:746	amorphous structures	727:746	amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites	727:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	4	49	theme	starch	639:644	arg1	content					583:589	improved content	574:589	improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	574:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	2	50	with	fermentation	241:252	arg1	BAK					301:303	BAK	301:303	BAK	301:303	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	2	50	with	fermentation	241:252	arg1	YAK					281:283	YAK	281:283	YAK	281:283	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	2	50	with	fermentation	241:252	arg1	buttermilk					289:298	buttermilk	289:298	buttermilk (BAK)	289:304	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	2	50	with	fermentation	241:252	arg1	yeast					274:278	yeast	274:278	yeast (YAK)	274:284	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	6	51	theme	animal	944:949	arg1	model					951:955	the animal model	940:955	the animal model	940:955	The extracts of khambir alleviated cold-induced gastric ulcers in the animal model as it exhibited histoprotective and anti-inflammatory activities.					
33321345	4	52	theme	starch	657:662	arg1	content					583:589	improved content	574:589	improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	574:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	4	53	theme	release	530:536	arg1	potential					538:546	a slow glucose-controlled release potential	504:546	a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	504:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	6	54	theme	cold-induced	909:920	arg1	ulcers					930:935	cold-induced gastric ulcers	909:935	cold-induced gastric ulcers	909:935	The extracts of khambir alleviated cold-induced gastric ulcers in the animal model as it exhibited histoprotective and anti-inflammatory activities.					
33321345	4	55	theme	resistant	647:655	arg1	starch					657:662	resistant starch	647:662	resistant starch	647:662	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	4	56	theme	glucose-controlled	511:528	arg1	potential					538:546	a slow glucose-controlled release potential	504:546	a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	504:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	0	57	theme	ulcer	73:77	arg1	potentialities					79:92	anti-gastric ulcer potentialities	60:92	anti-gastric ulcer potentialities of khambir	60:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	2	58	theme	sourdough	231:239	arg1	fermentation					241:252	sourdough fermentation	231:252	sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK)	231:304	It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK).					
33321345	6	59	theme	histoprotective	973:987	arg1	activities					1011:1020	histoprotective and anti-inflammatory activities	973:1020	histoprotective and anti-inflammatory activities	973:1020	The extracts of khambir alleviated cold-induced gastric ulcers in the animal model as it exhibited histoprotective and anti-inflammatory activities.					
33321345	5	60	theme	accumulation	798:809	arg1	structures					737:746	amorphous structures	727:746	amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites	727:833	The changes of crystallinity to amorphous structures of starch, content of protein and fatty acid, and accumulation of 17 major metabolites were evaluated through FTIR and GC-MS.					
33321345	0	61	theme	anti-gastric	60:71	arg1	potentialities					79:92	anti-gastric ulcer potentialities	60:92	anti-gastric ulcer potentialities of khambir	60:103	Evaluation of nutrient profile, biochemical composition and anti-gastric ulcer potentialities of khambir, a leavened flat bread.					
33321345	3	62	from	carbohydrate	340:351	arg1	rich					332:335	rich	332:335	rich	332:335	Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals.					
33321345	4	63	theme	starch	613:618	arg1	content					583:589	improved content	574:589	improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index	574:692	The in vitro digestibility test showed a slow glucose-controlled release potential of khambir that reflected improved content of rapidly digestible starch, slowly digestible starch, resistant starch, and predicted glycemic index.					
33321345	7	64	theme	mountain	1156:1163	arg1	sickness					1165:1172	acute mountain sickness	1150:1172	acute mountain sickness	1150:1172	This study demonstrated that the traditional leavened bread khambir is nutritious and can alleviate gastric lesions related to acute mountain sickness.					
33287433	0	0	theme	Chitosan-Poly	83:95	arg1	Glycol					130:135	Ethylene Glycol	121:135	Ethylene Glycol	121:135	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	0	0	theme	Chitosan-Poly	83:95	arg1	-Poly					115:119	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly	83:119	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	83:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	1	1	theme	acrylic	296:302	arg1	acid					304:307	acrylic acid	296:307	acrylic acid	296:307	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	1	theme	acrylic	296:302	arg1	-Poly					290:294	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly	234:294	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid)	234:308	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	2	2	theme	swelling	536:543	arg1	studies					545:551	sol-gel and swelling studies	524:551	sol-gel and swelling studies	524:551	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	6	3	from	%	1438:1438	arg1	2h					1453:1454	the first 2h	1443:1454	the first 2h	1443:1454	Simultaneously, IBU was released up to 25% in the first 2h, having a release maximum after 8 h.					
33287433	2	4	theme	irradiation	772:782	arg1	dose					784:787	the irradiation dose	768:787	the irradiation dose	768:787	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	3	5	with	Hydrogels	864:872	arg1	fraction					883:890	gel fraction	879:890	gel fraction above 85%	879:900	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	3	6	theme	swelling	915:922	arg1	properties					924:933	the best swelling properties	906:933	the best swelling properties in different pH solutions	906:959	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	3	7	from	properties	924:933	arg1	solutions					951:959	different pH solutions	938:959	different pH solutions	938:959	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	2	8	from	size	836:839	arg1	nm					860:861	the range 11-67 nm	844:861	the range 11-67 nm	844:861	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	0	9	theme	Vinyl-Pyrrolidone	97:113	arg1	Glycol					130:135	Ethylene Glycol	121:135	Ethylene Glycol	121:135	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	0	9	theme	Vinyl-Pyrrolidone	97:113	arg1	-Poly					115:119	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly	83:119	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	83:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	4	10	theme	appropriate	1107:1117	arg1	capability					1138:1147	appropriate moisture retention capability	1107:1147	appropriate moisture retention capability	1107:1147	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	2	11	theme	sol-gel	524:530	arg1	studies					545:551	sol-gel and swelling studies	524:551	sol-gel and swelling studies	524:551	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	3	12	theme	different	938:946	arg1	solutions					951:959	different pH solutions	938:959	different pH solutions	938:959	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	1	13	theme	Chitosan-Poly	234:246	arg1	-Poly					268:272	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly	234:272	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid)	234:308	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	13	theme	Chitosan-Poly	234:246	arg1	glycol					283:288	ethylene glycol	274:288	ethylene glycol	274:288	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	2	14	theme	components	656:665	arg1	interaction					628:638	the efficient interaction	614:638	the efficient interaction of the hydrogel components	614:665	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	2	15	theme	efficient	618:626	arg1	interaction					628:638	the efficient interaction	614:638	the efficient interaction of the hydrogel components	614:665	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	4	16	theme	vapor	1163:1167	arg1	rate					1182:1185	the water vapor transmission rate	1153:1185	the water vapor transmission rate up to 272.67 g m-2 day-1	1153:1210	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	4	17	theme	physiological	1053:1065	arg1	condition					1067:1075	the simulated physiological condition	1039:1075	the simulated physiological condition of an infected wound	1039:1096	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	4	18	dep	272.67	1193:1198	arg1	to					1190:1191	to	1190:1191	to	1190:1191	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	0	19	theme	-Poly	115:119	arg1	-Poly					137:141	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly	83:141	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	83:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	0	19	theme	-Poly	115:119	arg1	Acid					151:154	Acrylic Acid	143:154	Acrylic Acid	143:154	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	4	20	theme	water	1157:1161	arg1	vapor					1163:1167	water vapor	1157:1167	the water vapor transmission rate up to 272.67 g m-2 day-1	1153:1210	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	5	21	theme	significant	1268:1278	arg1	capacity					1288:1295	a significant loading capacity	1266:1295	a significant loading capacity of ibuprofen (IBU)	1266:1314	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	4	22	theme	fast	1223:1226	arg1	healing					1228:1234	fast healing	1223:1234	fast healing	1223:1234	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	2	23	theme	network	554:560	arg1	parameters					562:571	network parameters	554:571	network parameters	554:571	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	3	24	theme	pH	948:949	arg1	solutions					951:959	different pH solutions	938:959	different pH solutions	938:959	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	4	25	theme	simulated	1043:1051	arg1	condition					1067:1075	the simulated physiological condition	1039:1075	the simulated physiological condition of an infected wound	1039:1096	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	6	26	dep	%	1438:1438	arg1	up					1430:1431	up	1430:1431	up	1430:1431	Simultaneously, IBU was released up to 25% in the first 2h, having a release maximum after 8 h.					
33287433	4	27	theme	retention	1128:1136	arg1	capability					1138:1147	appropriate moisture retention capability	1107:1147	appropriate moisture retention capability	1107:1147	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	1	28	theme	ethylene	319:326	arg1	Poly					314:317	Poly	314:317	Poly(ethylene oxide)	314:333	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	28	theme	ethylene	319:326	arg1	oxide					328:332	ethylene oxide	319:332	ethylene oxide	319:332	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	5	29	theme	therapeutic	1345:1355	arg1	dose					1357:1360	a therapeutic dose	1343:1360	a therapeutic dose for the treatment of severe pains	1343:1394	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	2	30	theme	XPS	594:596	arg1	analyses					598:605	FTIR and XPS analyses	585:605	analyses	598:605	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	2	31	with	structure	812:820	arg1	size					836:839	the mesh size	827:839	the mesh size in the range 11-67 nm	827:861	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	5	32	theme	pains	1390:1394	arg1	treatment					1370:1378	the treatment	1366:1378	the treatment of severe pains	1366:1394	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	2	33	theme	structure	812:820	arg1	formation					797:805	the formation	793:805	the formation of a structure with the mesh size in the range 11-67 nm	793:861	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	2	33	theme	structure	812:820	arg1	dose					784:787	the irradiation dose	768:787	the irradiation dose	768:787	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	1	34	theme	N-Vinyl-Pyrrolidone	248:266	arg1	-Poly					268:272	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly	234:272	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid)	234:308	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	34	theme	N-Vinyl-Pyrrolidone	248:266	arg1	glycol					283:288	ethylene glycol	274:288	ethylene glycol	274:288	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	35	theme	rapid	398:402	arg1	healing					404:410	rapid healing	398:410	rapid healing	398:410	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	4	36	from	stable	1029:1034	arg1	condition					1067:1075	the simulated physiological condition	1039:1075	the simulated physiological condition of an infected wound	1039:1096	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	0	37	theme	Radiation	16:24	arg1	Cross-Linking					26:38	Radiation Cross-Linking	16:38	Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	16:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	0	38	theme	Ethylene	121:128	arg1	Glycol					130:135	Ethylene Glycol	121:135	Ethylene Glycol	121:135	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	0	38	theme	Ethylene	121:128	arg1	-Poly					115:119	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly	83:119	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	83:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	2	39	theme	FTIR	585:588	arg1	analyses					598:605	FTIR and XPS analyses	585:605	analyses	598:605	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	6	40	theme	first	1447:1451	arg1	2h					1453:1454	the first 2h	1443:1454	the first 2h	1443:1454	Simultaneously, IBU was released up to 25% in the first 2h, having a release maximum after 8 h.					
33287433	3	41	from	Hydrogels	864:872	arg1	solutions					951:959	different pH solutions	938:959	different pH solutions	938:959	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	2	42	theme	hydrogel	647:654	arg1	components					656:665	the hydrogel components	643:665	the hydrogel components	643:665	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	1	43	theme	-Poly	268:272	arg1	acid					304:307	acrylic acid	296:307	acrylic acid	296:307	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	43	theme	-Poly	268:272	arg1	-Poly					290:294	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly	234:294	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid)	234:308	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	4	44	theme	g	1200:1200	arg1	day-1					1206:1210	up to 272.67 g m-2 day-1	1187:1210	the water vapor transmission rate up to 272.67 g m-2 day-1	1153:1210	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	5	45	theme	ibuprofen	1300:1308	arg1	capacity					1288:1295	a significant loading capacity	1266:1295	a significant loading capacity of ibuprofen (IBU)	1266:1314	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	2	46	theme	bonding	701:707	arg1	environment					709:719	the bonding environment	697:719	the bonding environment	697:719	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	2	47	theme	mesh	831:834	arg1	size					836:839	the mesh size	827:839	the mesh size in the range 11-67 nm	827:861	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	5	48	theme	loading	1280:1286	arg1	capacity					1288:1295	a significant loading capacity	1266:1295	a significant loading capacity of ibuprofen (IBU)	1266:1314	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	1	49	theme	successful	175:184	arg1	preparation					186:196	the successful preparation	171:196	the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media	171:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	50	theme	ethylene	274:281	arg1	-Poly					268:272	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly	234:272	Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid)	234:308	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	50	theme	ethylene	274:281	arg1	glycol					283:288	ethylene glycol	274:288	ethylene glycol	274:288	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	4	51	dep	rate	1182:1185	arg1	day-1					1206:1210	up to 272.67 g m-2 day-1	1187:1210	the water vapor transmission rate up to 272.67 g m-2 day-1	1153:1210	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	3	52	theme	gel	879:881	arg1	fraction					883:890	gel fraction	879:890	gel fraction above 85%	879:900	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	3	53	with	properties	924:933	arg1	fraction					883:890	gel fraction	879:890	gel fraction above 85%	879:900	Hydrogels with gel fraction above 85% and the best swelling properties in different pH solutions were obtained for hydrogels produced with 15 kGy.					
33287433	0	54	theme	Hydrogels	54:62	arg1	Dressings					64:72	Quaternary Hydrogels Dressings	43:72	Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	43:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	2	55	dep	structure	458:466	arg1	The					454:456	The	454:456	The	454:456	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	5	56	theme	severe	1383:1388	arg1	pains					1390:1394	severe pains	1383:1394	severe pains	1383:1394	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	1	57	theme	e-beam	338:343	arg1	cross-linking					345:357	e-beam cross-linking	338:357	e-beam cross-linking in weakly acidic media	338:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	58	theme	pain	416:419	arg1	release					421:427	pain release	416:427	pain release of infected skin wounds	416:451	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	0	59	theme	Quaternary	43:52	arg1	Dressings					64:72	Quaternary Hydrogels Dressings	43:72	Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	43:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	4	60	theme	infected	1083:1090	arg1	wound					1092:1096	an infected wound	1080:1096	an infected wound	1080:1096	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	4	61	theme	transmission	1169:1180	arg1	rate					1182:1185	the water vapor transmission rate	1153:1185	the water vapor transmission rate up to 272.67 g m-2 day-1	1153:1210	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	1	62	from	cross-linking	345:357	arg1	media					376:380	weakly acidic media	362:380	weakly acidic media	362:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	2	63	theme	range	848:852	arg1	nm					860:861	the range 11-67 nm	844:861	the range 11-67 nm	844:861	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	2	64	theme	hydrogels	488:496	arg1	compositions					472:483	compositions	472:483	compositions	472:483	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	2	64	theme	hydrogels	488:496	arg1	structure					458:466	structure	458:466	structure	458:466	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	4	65	theme	moisture	1119:1126	arg1	capability					1138:1147	appropriate moisture retention capability	1107:1147	appropriate moisture retention capability	1107:1147	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	0	66	theme	Acrylic	143:149	arg1	-Poly					137:141	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly	83:141	Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	83:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	0	66	theme	Acrylic	143:149	arg1	Acid					151:154	Acrylic Acid	143:154	Acrylic Acid	143:154	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	4	67	theme	wound	1092:1096	arg1	condition					1067:1075	the simulated physiological condition	1039:1075	the simulated physiological condition of an infected wound	1039:1096	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	1	68	theme	infected	432:439	arg1	wounds					446:451	infected skin wounds	432:451	infected skin wounds	432:451	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	6	69	theme	release	1466:1472	arg1	maximum					1474:1480	a release maximum	1464:1480	a release maximum	1464:1480	Simultaneously, IBU was released up to 25% in the first 2h, having a release maximum after 8 h.					
33287433	2	70	theme	cross-linking	731:743	arg1	degree					745:750	the cross-linking degree	727:750	the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm	727:861	The structure and compositions of hydrogels investigated according to sol-gel and swelling studies, network parameters, as well as FTIR and XPS analyses showed the efficient interaction of the hydrogel components upon irradiation, maintaining the bonding environment while the cross-linking degree increasing with the irradiation dose and the formation of a structure with the mesh size in the range 11-67 nm.					
33287433	1	71	theme	wet	201:203	arg1	hydrogels					215:223	wet dressings hydrogels	201:223	wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media	201:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	72	theme	acidic	369:374	arg1	media					376:380	weakly acidic media	362:380	weakly acidic media	362:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	4	73	from	condition	1067:1075	arg1	hydrogels					1015:1023	The hydrogels	1011:1023	The hydrogels	1011:1023	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	4	73	from	condition	1067:1075	arg1	stable					1029:1034	stable	1029:1034	stable	1029:1034	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	1	74	theme	skin	441:444	arg1	wounds					446:451	infected skin wounds	432:451	infected skin wounds	432:451	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	75	used	used	389:392	arg2	We					158:159	We	158:159	We	158:159	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	76	theme	dressings	205:213	arg1	hydrogels					215:223	wet dressings hydrogels	201:223	wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media	201:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	0	77	theme	Dressings	64:72	arg1	Cross-Linking					26:38	Radiation Cross-Linking	16:38	Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid)	16:155	One Step e-Beam Radiation Cross-Linking of Quaternary Hydrogels Dressings Based on Chitosan-Poly(Vinyl-Pyrrolidone)-Poly(Ethylene Glycol)-Poly(Acrylic Acid).					
33287433	1	78	theme	wounds	446:451	arg1	release					421:427	pain release	416:427	pain release of infected skin wounds	416:451	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	1	78	theme	wounds	446:451	arg1	healing					404:410	rapid healing	398:410	rapid healing	398:410	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
33287433	5	79	contain	have	1261:1264	arg2	capacity					1288:1295	a significant loading capacity	1266:1295	a significant loading capacity of ibuprofen (IBU)	1266:1314	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	5	79	contain	have	1261:1264	arg1	hydrogels					1241:1249	The hydrogels	1237:1249	The hydrogels	1237:1249	The hydrogels proved to have a significant loading capacity of ibuprofen (IBU), being able to incorporate a therapeutic dose for the treatment of severe pains.					
33287433	4	80	theme	m-2	1202:1204	arg1	day-1					1206:1210	up to 272.67 g m-2 day-1	1187:1210	the water vapor transmission rate up to 272.67 g m-2 day-1	1153:1210	The hydrogels are stable in the simulated physiological condition of an infected wound and show appropriate moisture retention capability and the water vapor transmission rate up to 272.67 g m-2 day-1, to ensure fast healing.					
33287433	1	81	theme	hydrogels	215:223	arg1	preparation					186:196	the successful preparation	171:196	the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media	171:380	We report on the successful preparation of wet dressings hydrogels based on Chitosan-Poly(N-Vinyl-Pyrrolidone)-Poly(ethylene glycol)-Poly(acrylic acid) and Poly(ethylene oxide) by e-beam cross-linking in weakly acidic media, to be used for rapid healing and pain release of infected skin wounds.					
32169768	0	0	theme	plant	85:89	arg1	Pursh					110:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	10	1	theme	polyphenol-enriched	1110:1128	arg1	chinense					1133:1140	polyphenol-enriched P. chinense	1110:1140	polyphenol-enriched P. chinense	1110:1140	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32169768	6	2	theme	elevated	720:727	arg1	ratio					754:758	elevated Bacteroidetes/Firmicutes ratio	720:758	elevated Bacteroidetes/Firmicutes ratio	720:758	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	1	3	theme	medicinal	155:163	arg1	Pursh					136:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	1	3	theme	medicinal	155:163	arg1	plant					165:169	a dietary medicinal plant	145:169	a dietary medicinal plant widely distributed in Asia-Pacific countries	145:214	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	7	4	theme	beneficial	901:910	arg1	bacteria					912:919	some beneficial bacteria	896:919	some beneficial bacteria	896:919	After a one-week drug-free wash, most of these changes were recovered, but the abundance of some beneficial bacteria was further increased.					
32169768	0	5	theme	medicinal	75:83	arg1	Pursh					110:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	4	6	theme	Total	428:432	arg1	flavonoid					434:442	Total flavonoid	428:442	Total flavonoid	428:442	Total flavonoid and phenolic contents of P. chinense were 46.6% and 61.3% (w/w), respectively.					
32169768	0	7	theme	chinense	101:108	arg1	Pursh					110:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	10	8	from	capacity	1192:1199	arg1	mice					1204:1207	mice	1204:1207	mice	1204:1207	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32169768	8	9	theme	several	995:1001	arg1	pathways					1013:1020	several metabolic pathways	995:1020	several metabolic pathways	995:1020	The altered composition of gut microbiota enriched several metabolic pathways.					
32169768	6	10	theme	P.	605:606	arg1	chinense					608:615	P. chinense	605:615	P. chinense	605:615	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	0	11	theme	Penthorum	91:99	arg1	Pursh					110:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	10	12	theme	gut	1152:1154	arg1	microbiota					1156:1165	gut microbiota	1152:1165	gut microbiota	1152:1165	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32169768	2	13	theme	chinense	283:290	arg1	constituents					264:275	the chemical constituents	251:275	the chemical constituents of P. chinense	251:290	The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota.					
32169768	0	14	theme	Pursh	110:114	arg1	composition					29:39	chemical composition	20:39	chemical composition	20:39	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	0	14	theme	Pursh	110:114	arg1	effect					55:60	prebiotic effect	45:60	prebiotic effect	45:60	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	2	15	theme	P.	280:281	arg1	chinense					283:290	P. chinense	280:290	P. chinense	280:290	The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota.					
32169768	6	16	theme	microbiota	698:707	arg1	diversity					709:717	increased microbiota diversity	688:717	increased microbiota diversity	688:717	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	6	17	theme	increased	688:696	arg1	diversity					709:717	increased microbiota diversity	688:717	increased microbiota diversity	688:717	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	8	18	theme	microbiota	975:984	arg1	composition					956:966	The altered composition	944:966	The altered composition of gut microbiota	944:984	The altered composition of gut microbiota enriched several metabolic pathways.					
32169768	1	19	theme	Asia-Pacific	193:204	arg1	countries					206:214	Asia-Pacific countries	193:214	Asia-Pacific countries	193:214	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	0	20	theme	composition	29:39	arg1	Characterization					0:15	Characterization	0:15	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.	0:115	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	10	21	theme	antioxidant	1180:1190	arg1	capacity					1192:1199	antioxidant capacity	1180:1199	antioxidant capacity in mice toward a beneficial environment for host health	1180:1255	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32169768	2	22	theme	prebiotic	312:320	arg1	role					322:325	its prebiotic role	308:325	its prebiotic role	308:325	The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota.					
32169768	8	23	theme	metabolic	1003:1011	arg1	pathways					1013:1020	several metabolic pathways	995:1020	several metabolic pathways	995:1020	The altered composition of gut microbiota enriched several metabolic pathways.					
32169768	0	24	theme	chemical	20:27	arg1	composition					29:39	chemical composition	20:39	chemical composition	20:39	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	7	25	theme	drug-free	821:829	arg1	wash					831:834	a one-week drug-free wash	810:834	a one-week drug-free wash	810:834	After a one-week drug-free wash, most of these changes were recovered, but the abundance of some beneficial bacteria was further increased.					
32169768	0	26	theme	prebiotic	45:53	arg1	effect					55:60	prebiotic effect	45:60	prebiotic effect	45:60	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	9	27	theme	antioxidant	1055:1065	arg1	capacity					1067:1074	increased antioxidant capacity	1045:1074	increased antioxidant capacity	1045:1074	Moreover, P. chinense increased antioxidant capacity in vivo.					
32169768	4	28	theme	chinense	472:479	arg1	flavonoid					434:442	Total flavonoid	428:442	Total flavonoid	428:442	Total flavonoid and phenolic contents of P. chinense were 46.6% and 61.3% (w/w), respectively.					
32169768	4	28	theme	chinense	472:479	arg1	contents					457:464	phenolic contents	448:464	phenolic contents	448:464	Total flavonoid and phenolic contents of P. chinense were 46.6% and 61.3% (w/w), respectively.					
32169768	9	29	theme	increased	1045:1053	arg1	capacity					1067:1074	increased antioxidant capacity	1045:1074	increased antioxidant capacity	1045:1074	Moreover, P. chinense increased antioxidant capacity in vivo.					
32169768	3	30	theme	polyphenolic	363:374	arg1	compounds					376:384	Fifty polyphenolic compounds	357:384	Fifty polyphenolic compounds	357:384	Fifty polyphenolic compounds were rapidly identified using UPLC-HR-MS.					
32169768	7	31	theme	one-week	812:819	arg1	wash					831:834	a one-week drug-free wash	810:834	a one-week drug-free wash	810:834	After a one-week drug-free wash, most of these changes were recovered, but the abundance of some beneficial bacteria was further increased.					
32169768	6	32	theme	health-promoting	777:792	arg1	bacteria					794:801	enriched gut health-promoting bacteria	764:801	enriched gut health-promoting bacteria	764:801	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	1	33	theme	Penthorum	117:125	arg1	Pursh					136:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	1	33	theme	Penthorum	117:125	arg1	plant					165:169	a dietary medicinal plant	145:169	a dietary medicinal plant widely distributed in Asia-Pacific countries	145:214	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	0	34	theme	effect	55:60	arg1	Characterization					0:15	Characterization	0:15	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.	0:115	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	2	35	theme	present	221:227	arg1	study					229:233	The present study	217:233	The present study	217:233	The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota.					
32169768	6	36	theme	gut	773:775	arg1	bacteria					794:801	enriched gut health-promoting bacteria	764:801	enriched gut health-promoting bacteria	764:801	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	1	37	theme	chinense	127:134	arg1	Pursh					136:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	1	37	theme	chinense	127:134	arg1	plant					165:169	a dietary medicinal plant	145:169	a dietary medicinal plant widely distributed in Asia-Pacific countries	145:214	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	6	38	theme	community	661:669	arg1	arrangement					636:646	structural arrangement	625:646	structural arrangement of microbial community	625:669	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	4	39	theme	phenolic	448:455	arg1	contents					457:464	phenolic contents	448:464	phenolic contents	448:464	Total flavonoid and phenolic contents of P. chinense were 46.6% and 61.3% (w/w), respectively.					
32169768	6	40	theme	enriched	764:771	arg1	bacteria					794:801	enriched gut health-promoting bacteria	764:801	enriched gut health-promoting bacteria	764:801	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	2	41	theme	gut	341:343	arg1	microbiota					345:354	gut microbiota	341:354	gut microbiota	341:354	The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota.					
32169768	6	42	theme	Bacteroidetes/Firmicutes	729:752	arg1	ratio					754:758	elevated Bacteroidetes/Firmicutes ratio	720:758	elevated Bacteroidetes/Firmicutes ratio	720:758	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	10	43	theme	host	1245:1248	arg1	health					1250:1255	host health	1245:1255	host health	1245:1255	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32169768	8	44	theme	altered	948:954	arg1	composition					956:966	The altered composition	944:966	The altered composition of gut microbiota	944:984	The altered composition of gut microbiota enriched several metabolic pathways.					
32169768	4	45	theme	P.	469:470	arg1	chinense					472:479	P. chinense	469:479	P. chinense	469:479	Total flavonoid and phenolic contents of P. chinense were 46.6% and 61.3% (w/w), respectively.					
32169768	8	46	theme	gut	971:973	arg1	microbiota					975:984	gut microbiota	971:984	gut microbiota	971:984	The altered composition of gut microbiota enriched several metabolic pathways.					
32169768	7	47	theme	bacteria	912:919	arg1	abundance					883:891	the abundance	879:891	the abundance of some beneficial bacteria	879:919	After a one-week drug-free wash, most of these changes were recovered, but the abundance of some beneficial bacteria was further increased.					
32169768	0	48	theme	dietary	67:73	arg1	Pursh					110:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	a dietary medicinal plant Penthorum chinense Pursh	65:114	Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense Pursh.					
32169768	2	49	theme	chemical	255:262	arg1	constituents					264:275	the chemical constituents	251:275	the chemical constituents of P. chinense	251:290	The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota.					
32169768	6	50	theme	microbial	651:659	arg1	community					661:669	microbial community	651:669	microbial community	651:669	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	10	51	theme	P.	1130:1131	arg1	chinense					1133:1140	polyphenol-enriched P. chinense	1110:1140	polyphenol-enriched P. chinense	1110:1140	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32169768	5	52	theme	individual	532:541	arg1	polyphenols					543:553	Thirteen individual polyphenols	523:553	Thirteen individual polyphenols	523:553	Thirteen individual polyphenols were quantified, which accounted for 33.1% (w/w).					
32169768	1	53	theme	dietary	147:153	arg1	Pursh					136:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh	117:140	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	1	53	theme	dietary	147:153	arg1	plant					165:169	a dietary medicinal plant	145:169	a dietary medicinal plant widely distributed in Asia-Pacific countries	145:214	Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries.					
32169768	6	54	theme	structural	625:634	arg1	arrangement					636:646	structural arrangement	625:646	structural arrangement of microbial community	625:669	P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria.					
32169768	10	55	theme	beneficial	1218:1227	arg1	environment					1229:1239	a beneficial environment	1216:1239	a beneficial environment for host health	1216:1255	The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.					
32723025	5	0	theme	milk	919:922	arg1	components					924:933	other milk components	913:933	other milk components	913:933	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	0	1	from	composition	9:19	arg1	cows					65:68	cows	65:68	cows	65:68	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	0	1	from	composition	9:19	arg1	camels					54:59	camels	54:59	camels	54:59	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	5	2	theme	other	913:917	arg1	components					924:933	other milk components	913:933	other milk components	913:933	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	5	3	theme	milk	957:960	arg1	density					946:952	the density	942:952	the density of milk	942:960	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	1	4	from	cows	127:130	arg1	milk					106:109	milk	106:109	milk from camels and cows	106:130	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	1	4	from	cows	127:130	arg1	samples					95:101	A hundred samples	85:101	A hundred samples of milk from camels and cows	85:130	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	3	5	theme	milk	477:480	arg1	samples					482:488	the milk samples	473:488	the milk samples with the highest occurrence (82.4%)	473:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	6	theme	semi-intensive	661:674	arg1	systems					676:682	semi-intensive systems	661:682	semi-intensive systems	661:682	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	7	dep	found	457:461	arg1	0.1 µg/kg					715:723	0.1 µg/kg	715:723	0.1 µg/kg	715:723	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	7	dep	found	457:461	arg1	%					689:689	15.6%	685:689	15.6% ranged between 0.05	685:709	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	8	theme	Aflatoxin	435:443	arg1	M1					450:451	Aflatoxin (AF) M1	435:451	Aflatoxin (AF) M1	435:451	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	1	9	from	camels	116:121	arg1	milk					106:109	milk	106:109	milk from camels and cows	106:130	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	1	9	from	camels	116:121	arg1	samples					95:101	A hundred samples	85:101	A hundred samples of milk from camels and cows	85:130	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	2	10	theme	nomadic	248:254	arg1	systems					256:262	traditional nomadic systems	236:262	traditional nomadic systems	236:262	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	2	11	theme	traditional	236:246	arg1	systems					256:262	traditional nomadic systems	236:262	traditional nomadic systems	236:262	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	3	12	theme	samples	482:488	arg1	%					468:468	33%	466:468	33% of the milk samples with the highest occurrence (82.4%)	466:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	12	theme	samples	482:488	arg1	samples					482:488	the milk samples	473:488	the milk samples with the highest occurrence (82.4%)	473:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	0	13	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	1	14	theme	chemical	150:157	arg1	composition					159:169	chemical composition	150:169	chemical composition	150:169	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	3	15	with	samples	482:488	arg1	occurrence					507:516	the highest occurrence	495:516	the highest occurrence (82.4%)	495:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	15	with	samples	482:488	arg1	%					523:523	82.4%	519:523	82.4%	519:523	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	4	16	theme	traditional	752:762	arg1	systems					772:778	traditional nomadic systems	752:778	traditional nomadic systems	752:778	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	1	17	from	samples	95:101	arg1	camels					116:121	camels	116:121	camels	116:121	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	1	17	from	samples	95:101	arg1	cows					127:130	cows	127:130	cows	127:130	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	5	18	from	content	869:875	arg1	milk					880:883	milk	880:883	milk	880:883	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	4	19	from	systems	772:778	arg1	milk					742:745	milk	742:745	milk from traditional nomadic systems	742:778	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	4	19	from	systems	772:778	arg1	samples					731:737	All samples	727:737	All samples of milk from traditional nomadic systems	727:778	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	0	20	dep	camels	54:59	arg1	milk					70:73	milk	70:73	milk	70:73	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	0	21	theme	Aflatoxin	38:46	arg1	M1					48:49	Aflatoxin M1	38:49	Aflatoxin M1	38:49	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	3	22	theme	AF	446:447	arg1	M1					450:451	Aflatoxin (AF) M1	435:451	Aflatoxin (AF) M1	435:451	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	0	23	from	cows	65:68	arg1	Sudan					78:82	Sudan	78:82	Sudan	78:82	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	1	24	theme	M1	175:176	arg1	contamination					178:190	M1 contamination	175:190	M1 contamination	175:190	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	2	25	theme	semi-intensive	411:424	arg1	systems					426:432	semi-intensive systems	411:432	semi-intensive systems	411:432	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	4	26	theme	nomadic	764:770	arg1	systems					772:778	traditional nomadic systems	752:778	traditional nomadic systems	752:778	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	3	27	theme	cow	529:531	arg1	milk					533:536	cow milk	529:536	cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems	529:682	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	5	28	theme	increased	855:863	arg1	content					869:875	increased fat content	855:875	increased fat content in milk	855:883	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	3	29	located	found	457:461	arg1	%					468:468	33%	466:468	33% of the milk samples with the highest occurrence (82.4%)	466:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	29	located	found	457:461	arg2	M1					450:451	Aflatoxin (AF) M1	435:451	Aflatoxin (AF) M1	435:451	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	29	located	found	457:461	arg1	samples					482:488	the milk samples	473:488	the milk samples with the highest occurrence (82.4%)	473:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	29	located	found	457:461	arg1	milk					533:536	cow milk	529:536	cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems	529:682	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	30	theme	milk	634:637	arg1	samples					639:645	milk samples	634:645	milk samples from camel in semi-intensive systems	634:682	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	0	31	from	camels	54:59	arg1	Sudan					78:82	Sudan	78:82	Sudan	78:82	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	5	32	theme	fat	865:867	arg1	content					869:875	increased fat content	855:875	increased fat content in milk	855:883	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	4	33	theme	milk	742:745	arg1	samples					731:737	All samples	727:737	All samples of milk from traditional nomadic systems	727:778	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	0	34	theme	M1	48:49	arg1	detection					25:33	detection	25:33	detection	25:33	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	0	34	theme	M1	48:49	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	3	35	theme	highest	499:505	arg1	occurrence					507:516	the highest occurrence	495:516	the highest occurrence (82.4%)	495:524	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	35	theme	highest	499:505	arg1	%					523:523	82.4%	519:523	82.4%	519:523	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	2	36	dep	significantly	273:285	arg1	P					288:288	P 0.05	288:293	P 0.05	288:293	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	5	37	with	AF	847:848	arg1	content					869:875	increased fat content	855:875	increased fat content in milk	855:883	These results revealed increasing of AF with increased fat content in milk, a decrease with decreasing other milk components, while the density of milk showed no relation.					
32723025	3	38	from	camel	652:656	arg1	systems					676:682	semi-intensive systems	661:682	semi-intensive systems	661:682	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	38	from	camel	652:656	arg1	samples					639:645	milk samples	634:645	milk samples from camel in semi-intensive systems	634:682	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	2	39	theme	higher	296:301	arg1	values					303:308	significantly (P 0.05) higher values	273:308	significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems	273:432	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	4	40	from	samples	731:737	arg1	systems					772:778	traditional nomadic systems	752:778	traditional nomadic systems	752:778	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	1	41	theme	milk	106:109	arg1	samples					95:101	A hundred samples	85:101	A hundred samples of milk from camels and cows	85:130	A hundred samples of milk from camels and cows were analysed for chemical composition and M1 contamination.					
32723025	3	42	dep	milk	533:536	arg1	%					586:586	47.1%	582:586	47.1% ranged between 0.1 and 0.15 µ/kg	582:619	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	3	42	dep	milk	533:536	arg1	%					543:543	35.3%	539:543	35.3% ranged between 0.05 and 0.1 µ/kg	539:576	Aflatoxin (AF) M1 was found in 33% of the milk samples with the highest occurrence (82.4%) in cow milk (35.3% ranged between 0.05 and 0.1 µ/kg and 47.1% ranged between 0.1 and 0.15 µ/kg) compared to milk samples from camel in semi-intensive systems (15.6% ranged between 0.05 and 0.1 µg/kg).					
32723025	2	43	theme	camel	220:224	arg1	milk					226:229	camel milk	220:229	camel milk from traditional nomadic systems	220:262	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	2	44	from	systems	256:262	arg1	milk					226:229	camel milk	220:229	camel milk from traditional nomadic systems	220:262	The results indicated that camel milk from traditional nomadic systems revealed significantly (P 0.05) higher values for protein, fat, lactose, solids-not-fat and density than that obtained from camel and cows kept in semi-intensive systems.					
32723025	4	45	theme	AFM1	804:807	arg1	absence					793:799	the absence	789:799	the absence of AFM1	789:807	All samples of milk from traditional nomadic systems revealed the absence of AFM1.					
32723025	0	46	from	detection	25:33	arg1	cows					65:68	cows	65:68	cows	65:68	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32723025	0	46	from	detection	25:33	arg1	camels					54:59	camels	54:59	camels	54:59	Chemical composition and detection of Aflatoxin M1 in camels and cows milk in Sudan.					
32693125	20	0	theme	offering	2588:2595	arg1	formulation					2636:2646	the offering, efficient safe and patient convenient formulation	2584:2646	the offering, efficient safe and patient convenient formulation for OA management	2584:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	20	1	theme	patches	2440:2446	arg1	effect					2416:2421	the OA protective effect	2398:2421	the OA protective effect of the developed patches	2398:2446	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	20	2	theme	nanoparticles/hydrogel	2550:2571	arg1	patches					2573:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	20	3	dep	offering	2588:2595	arg1	efficient					2598:2606	efficient	2598:2606	efficient	2598:2606	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	20	4	from	potentiality	2491:2502	arg1	formulation					2636:2646	the offering, efficient safe and patient convenient formulation	2584:2646	the offering, efficient safe and patient convenient formulation for OA management	2584:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	19	5	theme	rat	2181:2183	arg1	model					2200:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	2	6	theme	therapeutic	285:295	arg1	avenues					297:303	the newly explored therapeutic avenues	266:303	the newly explored therapeutic avenues that were opened by its application in serious ailments	266:359	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	18	7	theme	drug	1862:1865	arg1	solution					1875:1882	free drug aqueous solution	1857:1882	free drug aqueous solution	1857:1882	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	17	8	theme	Ex	1769:1770	arg1	study					1793:1797	Ex vivo drug permeation study	1769:1797	Ex vivo drug permeation study through isolated rat skin	1769:1823	Ex vivo drug permeation study through isolated rat skin was conducted.					
32693125	2	9	dep	due	259:261	arg1	nowadays					250:257	nowadays	250:257	nowadays	250:257	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	6	10	contain	has	685:687	arg2	effect					740:745	extensive first-pass effect	719:745	extensive first-pass effect	719:745	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	10	contain	has	685:687	arg2	bioavailability					753:767	poor bioavailability	748:767	poor bioavailability	748:767	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	10	contain	has	685:687	arg1	administration					670:683	Colchicine oral administration	654:683	Colchicine oral administration	654:683	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	10	contain	has	685:687	arg2	effects					790:796	severe GIT side effects	774:796	severe GIT side effects	774:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	10	contain	has	685:687	arg2	limitations					697:707	several limitations	689:707	several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects	689:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	17	11	theme	drug	1777:1780	arg1	study					1793:1797	Ex vivo drug permeation study	1769:1797	Ex vivo drug permeation study through isolated rat skin	1769:1823	Ex vivo drug permeation study through isolated rat skin was conducted.					
32693125	14	12	theme	adsorption-desorption	1513:1533	arg1	isotherms					1535:1543	Nitrogen adsorption-desorption isotherms	1504:1543	Nitrogen adsorption-desorption isotherms	1504:1543	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	9	13	theme	novel	992:996	arg1	systems					1030:1036	novel colchicine transdermal delivery systems	992:1036	novel colchicine transdermal delivery systems	992:1036	In this study, novel colchicine transdermal delivery systems were developed to conquer such obstacles.					
32693125	19	14	theme	improved	2215:2222	arg1	activity					2234:2241	improved locomotor activity	2215:2241	improved locomotor activity	2215:2241	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	1	15	theme	bright	198:203	arg1	shining					205:211	a bright shining future	196:218	a bright shining future	196:218	Colchicine is a drug from the past with a bright shining future.					
32693125	9	16	theme	transdermal	1009:1019	arg1	systems					1030:1036	novel colchicine transdermal delivery systems	992:1036	novel colchicine transdermal delivery systems	992:1036	In this study, novel colchicine transdermal delivery systems were developed to conquer such obstacles.					
32693125	20	17	theme	protective	2405:2414	arg1	effect					2416:2421	the OA protective effect	2398:2421	the OA protective effect of the developed patches	2398:2446	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	1	18	theme	future	213:218	arg1	shining					205:211	a bright shining future	196:218	a bright shining future	196:218	Colchicine is a drug from the past with a bright shining future.					
32693125	19	19	from	activity	2234:2241	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	13	20	theme	cotton	1419:1424	arg1	fabric					1426:1431	cotton fabric	1419:1431	cotton fabric	1419:1431	These composites were used to treat cotton fabric to produce easily applicable and extended-release transdermal patches.					
32693125	19	21	theme	glutathione	2244:2254	arg1	level					2262:2266	glutathione blood level	2244:2266	glutathione blood level	2244:2266	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	16	22	theme	cotton	1723:1728	arg1	patch					1730:1734	The prepared cotton patch	1710:1734	The prepared cotton patch	1710:1734	The prepared cotton patch was tested for fabric stiffness.					
32693125	2	23	theme	much	235:238	arg1	attention					240:248	much attention	235:248	much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments	235:359	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	20	24	theme	colchicine	2521:2530	arg1	patches					2573:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	14	25	theme	MSNs	1653:1656	arg1	area					1594:1597	surface area	1586:1597	surface area	1586:1597	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	25	theme	MSNs	1653:1656	arg1	size					1613:1616	size	1613:1616	size	1613:1616	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	25	theme	MSNs	1653:1656	arg1	potential					1624:1632	zeta potential	1619:1632	zeta potential	1619:1632	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	25	theme	MSNs	1653:1656	arg1	morphology					1639:1648	morphology	1639:1648	morphology	1639:1648	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	25	theme	MSNs	1653:1656	arg1	pore-volume					1600:1610	pore-volume	1600:1610	pore-volume	1600:1610	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	20	26	theme	joint	2382:2386	arg1	Histopathology					2354:2367	Histopathology	2354:2367	Histopathology of rats knee joint	2354:2386	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	20	27	theme	silica	2543:2548	arg1	patches					2573:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	19	28	from	model	2200:2204	arg1	investigation					2100:2112	The therapeutic investigation	2084:2112	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model	2084:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	9	29	theme	conquer	1056:1062	arg1	obstacles					1069:1077	conquer such obstacles	1056:1077	conquer such obstacles	1056:1077	In this study, novel colchicine transdermal delivery systems were developed to conquer such obstacles.					
32693125	20	30	theme	mesoporous	2532:2541	arg1	patches					2573:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	10	31	theme	Cellulose-based	1080:1094	arg1	patches					1096:1102	Cellulose-based patches	1080:1102	Cellulose-based patches	1080:1102	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	18	32	theme	laser	2048:2052	arg1	microscopy					2054:2063	confocal laser microscopy	2039:2063	confocal laser microscopy using fluorescein	2039:2081	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	10	33	theme	mesoporous	1123:1132	arg1	nanoparticles					1141:1153	mesoporous silica nanoparticles	1123:1153	mesoporous silica nanoparticles (MSNs)	1123:1160	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	33	theme	mesoporous	1123:1132	arg1	MSNs					1156:1159	MSNs	1156:1159	MSNs	1156:1159	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	33	theme	mesoporous	1123:1132	arg1	encapsulators					1199:1211	colchicine encapsulators	1188:1211	colchicine encapsulators	1188:1211	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	19	34	theme	formulated	2128:2137	arg1	patches					2139:2145	colchicine formulated patches	2117:2145	colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model	2117:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	1	35	from	past	186:189	arg1	Colchicine					156:165	Colchicine	156:165	Colchicine	156:165	Colchicine is a drug from the past with a bright shining future.					
32693125	1	35	from	past	186:189	arg1	drug					172:175	a drug	170:175	a drug from the past with a bright shining future	170:218	Colchicine is a drug from the past with a bright shining future.					
32693125	8	36	theme	transdermal	872:882	arg1	delivery					884:891	colchicine transdermal delivery	861:891	colchicine transdermal delivery	861:891	However, colchicine transdermal delivery is challenging owing to its high aqueous solubility and hence poor skin permeation.					
32693125	19	37	from	decline	2284:2290	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	14	38	theme	zeta	1619:1622	arg1	potential					1624:1632	zeta potential	1619:1632	zeta potential	1619:1632	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	0	39	theme	new	89:91	arg1	system					106:111	a new encapsulator system	87:111	a new encapsulator system for transdermal osteoarthritis management	87:153	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	0	39	theme	new	89:91	arg1	patches					76:82	cotton patches	69:82	cotton patches	69:82	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	19	40	dep	formulated	2128:2137	arg1	colchicine					2117:2126	colchicine	2117:2126	colchicine	2117:2126	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	4	41	theme	extensive	508:516	arg1	pain					518:521	pain	518:521	pain	518:521	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	19	42	theme	oxide	2329:2333	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	20	43	theme	developed	2430:2438	arg1	patches					2440:2446	the developed patches	2426:2446	the developed patches	2426:2446	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	1	44	with	drug	172:175	arg1	shining					205:211	a bright shining future	196:218	a bright shining future	196:218	Colchicine is a drug from the past with a bright shining future.					
32693125	5	45	theme	different	608:616	arg1	cytokines					618:626	different cytokines	608:626	different cytokines like interleukin 6 (IL6)	608:651	Colchicine has been discovered to affect bone turnover and to reduce different cytokines like interleukin 6 (IL6).					
32693125	20	46	theme	safe	2608:2611	arg1	formulation					2636:2646	the offering, efficient safe and patient convenient formulation	2584:2646	the offering, efficient safe and patient convenient formulation for OA management	2584:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	19	47	from	level	2262:2266	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	4	48	theme	widespread	458:467	arg1	OA					450:451	OA	450:451	OA	450:451	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	4	48	theme	widespread	458:467	arg1	disease					488:494	a widespread joint degenerative disease	456:494	a widespread joint degenerative disease that causes extensive pain and disability	456:536	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	6	49	theme	extensive	719:727	arg1	effect					740:745	extensive first-pass effect	719:745	extensive first-pass effect	719:745	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	0	50	theme	transdermal	117:127	arg1	management					144:153	transdermal osteoarthritis management	117:153	transdermal osteoarthritis management	117:153	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	18	51	theme	24 h.	1991:1995	arg1	permeation					2002:2011	24 h. Skin permeation	1991:2011	24 h. Skin permeation	1991:2011	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	12	52	theme	carboxyethyl	1338:1349	arg1	chitosan					1351:1358	carboxyethyl chitosan	1338:1358	carboxyethyl chitosan	1338:1358	The hydrogel was prepared by reacting carboxyethyl chitosan and oxidized pullulan.					
32693125	4	53	theme	degenerative	475:486	arg1	OA					450:451	OA	450:451	OA	450:451	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	4	53	theme	degenerative	475:486	arg1	disease					488:494	a widespread joint degenerative disease	456:494	a widespread joint degenerative disease that causes extensive pain and disability	456:536	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	0	54	theme	Colchicine	0:9	arg1	composite					52:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	8	55	theme	aqueous	926:932	arg1	solubility					934:943	its high aqueous solubility	917:943	its high aqueous solubility	917:943	However, colchicine transdermal delivery is challenging owing to its high aqueous solubility and hence poor skin permeation.					
32693125	0	56	theme	silica	22:27	arg1	composite					52:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	7	57	theme	transdermal	803:813	arg1	route					815:819	The transdermal route	799:819	The transdermal route	799:819	The transdermal route circumvents these limitations.					
32693125	6	58	theme	oral	665:668	arg1	administration					670:683	Colchicine oral administration	654:683	Colchicine oral administration	654:683	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	19	59	from	investigation	2100:2112	arg1	model					2200:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	8	60	theme	poor	955:958	arg1	permeation					965:974	poor skin permeation	955:974	poor skin permeation	955:974	However, colchicine transdermal delivery is challenging owing to its high aqueous solubility and hence poor skin permeation.					
32693125	6	61	theme	severe	774:779	arg1	effects					790:796	severe GIT side effects	774:796	severe GIT side effects	774:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	2	62	theme	serious	344:350	arg1	ailments					352:359	serious ailments	344:359	serious ailments	344:359	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	0	63	theme	cotton	69:74	arg1	system					106:111	a new encapsulator system	87:111	a new encapsulator system for transdermal osteoarthritis management	87:153	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	0	63	theme	cotton	69:74	arg1	patches					76:82	cotton patches	69:82	cotton patches	69:82	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	20	64	theme	obtained	2453:2460	arg1	results					2462:2468	The obtained results	2449:2468	The obtained results	2449:2468	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	17	65	theme	rat	1816:1818	arg1	skin					1820:1823	isolated rat skin	1807:1823	isolated rat skin	1807:1823	Ex vivo drug permeation study through isolated rat skin was conducted.					
32693125	17	66	dep	Ex	1769:1770	arg1	vivo					1772:1775	vivo	1772:1775	vivo	1772:1775	Ex vivo drug permeation study through isolated rat skin was conducted.					
32693125	6	67	theme	poor	748:751	arg1	bioavailability					753:767	poor bioavailability	748:767	poor bioavailability	748:767	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	13	68	theme	transdermal	1483:1493	arg1	patches					1495:1501	easily applicable and extended-release transdermal patches	1444:1501	easily applicable and extended-release transdermal patches	1444:1501	These composites were used to treat cotton fabric to produce easily applicable and extended-release transdermal patches.					
32693125	20	69	theme	rats	2372:2375	arg1	joint					2382:2386	rats knee joint	2372:2386	rats knee joint	2372:2386	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	19	70	theme	osteoarthritis	2185:2198	arg1	model					2200:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	14	71	used	used	1569:1572	arg2	DLS					1546:1548	DLS	1546:1548	DLS	1546:1548	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	71	used	used	1569:1572	arg2	isotherms					1535:1543	Nitrogen adsorption-desorption isotherms	1504:1543	Nitrogen adsorption-desorption isotherms	1504:1543	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	71	used	used	1569:1572	arg2	SEM					1560:1562	SEM	1560:1562	SEM	1560:1562	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	14	71	used	used	1569:1572	arg2	TEM					1551:1553	TEM	1551:1553	TEM	1551:1553	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	2	72	from	application	329:339	arg1	ailments					352:359	serious ailments	344:359	serious ailments	344:359	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	19	73	theme	-induced	2172:2179	arg1	model					2200:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	20	74	theme	convenient	2625:2634	arg1	formulation					2636:2646	the offering, efficient safe and patient convenient formulation	2584:2646	the offering, efficient safe and patient convenient formulation for OA management	2584:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	19	75	theme	locomotor	2224:2232	arg1	activity					2234:2241	improved locomotor activity	2215:2241	improved locomotor activity	2215:2241	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	2	76	theme	explored	276:283	arg1	avenues					297:303	the newly explored therapeutic avenues	266:303	the newly explored therapeutic avenues that were opened by its application in serious ailments	266:359	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	18	77	theme	free	1857:1860	arg1	solution					1875:1882	free drug aqueous solution	1857:1882	free drug aqueous solution	1857:1882	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	19	78	from	patches	2139:2145	arg1	model					2200:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	mono-iodoacetate (MIA)-induced rat osteoarthritis model	2150:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	20	79	theme	developed	2511:2519	arg1	patches					2573:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	the developed colchicine mesoporous silica nanoparticles/hydrogel patches	2507:2579	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	17	80	theme	permeation	1782:1791	arg1	study					1793:1797	Ex vivo drug permeation study	1769:1797	Ex vivo drug permeation study through isolated rat skin	1769:1823	Ex vivo drug permeation study through isolated rat skin was conducted.					
32693125	14	81	theme	Nitrogen	1504:1511	arg1	isotherms					1535:1543	Nitrogen adsorption-desorption isotherms	1504:1543	Nitrogen adsorption-desorption isotherms	1504:1543	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	9	82	theme	colchicine	998:1007	arg1	systems					1030:1036	novel colchicine transdermal delivery systems	992:1036	novel colchicine transdermal delivery systems	992:1036	In this study, novel colchicine transdermal delivery systems were developed to conquer such obstacles.					
32693125	13	83	theme	applicable	1451:1460	arg1	patches					1495:1501	easily applicable and extended-release transdermal patches	1444:1501	easily applicable and extended-release transdermal patches	1444:1501	These composites were used to treat cotton fabric to produce easily applicable and extended-release transdermal patches.					
32693125	19	84	theme	blood	2256:2260	arg1	level					2262:2266	glutathione blood level	2244:2266	glutathione blood level	2244:2266	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	6	85	theme	side	785:788	arg1	effects					790:796	severe GIT side effects	774:796	severe GIT side effects	774:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	9	86	theme	delivery	1021:1028	arg1	systems					1030:1036	novel colchicine transdermal delivery systems	992:1036	novel colchicine transdermal delivery systems	992:1036	In this study, novel colchicine transdermal delivery systems were developed to conquer such obstacles.					
32693125	13	87	theme	extended-release	1466:1481	arg1	patches					1495:1501	easily applicable and extended-release transdermal patches	1444:1501	easily applicable and extended-release transdermal patches	1444:1501	These composites were used to treat cotton fabric to produce easily applicable and extended-release transdermal patches.					
32693125	20	88	theme	OA	2402:2403	arg1	effect					2416:2421	the OA protective effect	2398:2421	the OA protective effect of the developed patches	2398:2446	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	18	89	theme	aqueous	1867:1873	arg1	solution					1875:1882	free drug aqueous solution	1857:1882	free drug aqueous solution	1857:1882	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	20	90	theme	OA	2652:2653	arg1	management					2655:2664	OA management	2652:2664	OA management	2652:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	19	91	theme	remarkable	2273:2282	arg1	decline					2284:2290	remarkable decline	2273:2290	remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2273:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	16	92	theme	prepared	1714:1721	arg1	patch					1730:1734	The prepared cotton patch	1710:1734	The prepared cotton patch	1710:1734	The prepared cotton patch was tested for fabric stiffness.					
32693125	19	93	theme	malondialdehyde	2305:2319	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	19	94	theme	COX-2	2347:2351	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	6	95	theme	Colchicine	654:663	arg1	administration					670:683	Colchicine oral administration	654:683	Colchicine oral administration	654:683	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	13	96	used	used	1405:1408	arg2	composites					1389:1398	These composites	1383:1398	These composites	1383:1398	These composites were used to treat cotton fabric to produce easily applicable and extended-release transdermal patches.					
32693125	20	97	theme	knee	2377:2380	arg1	joint					2382:2386	rats knee joint	2372:2386	rats knee joint	2372:2386	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	18	98	theme	Skin	1997:2000	arg1	permeation					2002:2011	24 h. Skin permeation	1991:2011	24 h. Skin permeation	1991:2011	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	9	99	theme	such	1064:1067	arg1	obstacles					1069:1077	conquer such obstacles	1056:1077	conquer such obstacles	1056:1077	In this study, novel colchicine transdermal delivery systems were developed to conquer such obstacles.					
32693125	2	100	theme	due	259:261	arg1	attention					240:248	much attention	235:248	much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments	235:359	It has gained much attention nowadays due to the newly explored therapeutic avenues that were opened by its application in serious ailments.					
32693125	18	101	theme	confocal	2039:2046	arg1	microscopy					2054:2063	confocal laser microscopy	2039:2063	confocal laser microscopy using fluorescein	2039:2081	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	18	102	theme	enhanced	1906:1913	arg1	flux					1920:1923	enhanced drug flux	1906:1923	enhanced drug flux	1906:1923	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	0	103	theme	encapsulator	93:104	arg1	system					106:111	a new encapsulator system	87:111	a new encapsulator system for transdermal osteoarthritis management	87:153	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	0	103	theme	encapsulator	93:104	arg1	patches					76:82	cotton patches	69:82	cotton patches	69:82	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	19	104	theme	nitric	2322:2327	arg1	oxide					2329:2333	nitric oxide	2322:2333	nitric oxide	2322:2333	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	8	105	theme	colchicine	861:870	arg1	delivery					884:891	colchicine transdermal delivery	861:891	colchicine transdermal delivery	861:891	However, colchicine transdermal delivery is challenging owing to its high aqueous solubility and hence poor skin permeation.					
32693125	19	106	theme	TNF-α	2336:2340	arg1	levels					2295:2300	levels	2295:2300	levels of malondialdehyde, nitric oxide, TNF-α, and COX-2	2295:2351	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	11	107	theme	free	1218:1221	arg1	colchicine					1223:1232	The free colchicine	1214:1232	The free colchicine	1214:1232	The free colchicine or the encapsulated drug was embedded into self-healing hydrogel.					
32693125	4	108	theme	joint	469:473	arg1	OA					450:451	OA	450:451	OA	450:451	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	4	108	theme	joint	469:473	arg1	disease					488:494	a widespread joint degenerative disease	456:494	a widespread joint degenerative disease that causes extensive pain and disability	456:536	OA is a widespread joint degenerative disease that causes extensive pain and disability.					
32693125	20	109	theme	significant	2479:2489	arg1	potentiality					2491:2502	significant potentiality	2479:2502	significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management	2479:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	6	110	theme	first-pass	729:738	arg1	effect					740:745	extensive first-pass effect	719:745	extensive first-pass effect	719:745	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	0	111	theme	osteoarthritis	129:142	arg1	management					144:153	transdermal osteoarthritis management	117:153	transdermal osteoarthritis management	117:153	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	18	112	theme	drug	1949:1952	arg1	levels					1954:1959	permeated drug levels	1939:1959	permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein	1939:2081	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	0	113	theme	mesoporous	11:20	arg1	composite					52:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	10	114	theme	silica	1134:1139	arg1	nanoparticles					1141:1153	mesoporous silica nanoparticles	1123:1153	mesoporous silica nanoparticles (MSNs)	1123:1160	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	114	theme	silica	1134:1139	arg1	MSNs					1156:1159	MSNs	1156:1159	MSNs	1156:1159	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	114	theme	silica	1134:1139	arg1	encapsulators					1199:1211	colchicine encapsulators	1188:1211	colchicine encapsulators	1188:1211	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	5	115	theme	bone	580:583	arg1	turnover					585:592	bone turnover	580:592	bone turnover	580:592	Colchicine has been discovered to affect bone turnover and to reduce different cytokines like interleukin 6 (IL6).					
32693125	11	116	theme	encapsulated	1241:1252	arg1	drug					1254:1257	the encapsulated drug	1237:1257	the encapsulated drug	1237:1257	The free colchicine or the encapsulated drug was embedded into self-healing hydrogel.					
32693125	12	117	theme	oxidized	1364:1371	arg1	pullulan					1373:1380	oxidized pullulan	1364:1380	oxidized pullulan	1364:1380	The hydrogel was prepared by reacting carboxyethyl chitosan and oxidized pullulan.					
32693125	14	118	theme	surface	1586:1592	arg1	area					1594:1597	surface area	1586:1597	surface area	1586:1597	Nitrogen adsorption-desorption isotherms, DLS, TEM, and SEM were used to estimate surface area, pore-volume, size, zeta potential, and morphology of MSNs.					
32693125	6	119	theme	several	689:695	arg1	effect					740:745	extensive first-pass effect	719:745	extensive first-pass effect	719:745	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	119	theme	several	689:695	arg1	bioavailability					753:767	poor bioavailability	748:767	poor bioavailability	748:767	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	119	theme	several	689:695	arg1	effects					790:796	severe GIT side effects	774:796	severe GIT side effects	774:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	6	119	theme	several	689:695	arg1	limitations					697:707	several limitations	689:707	several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects	689:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	0	120	theme	nanoparticles/hydrogel	29:50	arg1	composite					52:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite	0:60	Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management.					
32693125	8	121	theme	high	921:924	arg1	solubility					934:943	its high aqueous solubility	917:943	its high aqueous solubility	917:943	However, colchicine transdermal delivery is challenging owing to its high aqueous solubility and hence poor skin permeation.					
32693125	11	122	theme	self-healing	1277:1288	arg1	hydrogel					1290:1297	self-healing hydrogel	1277:1297	self-healing hydrogel	1277:1297	The free colchicine or the encapsulated drug was embedded into self-healing hydrogel.					
32693125	10	123	used	used	1180:1183	arg2	encapsulators					1199:1211	colchicine encapsulators	1188:1211	colchicine encapsulators	1188:1211	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	123	used	used	1180:1183	arg2	MSNs					1156:1159	MSNs	1156:1159	MSNs	1156:1159	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	123	used	used	1180:1183	arg2	nanoparticles					1141:1153	mesoporous silica nanoparticles	1123:1153	mesoporous silica nanoparticles (MSNs)	1123:1160	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	6	124	theme	GIT	781:783	arg1	effects					790:796	severe GIT side effects	774:796	severe GIT side effects	774:796	Colchicine oral administration has several limitations including extensive first-pass effect, poor bioavailability, and severe GIT side effects.					
32693125	19	125	theme	therapeutic	2088:2098	arg1	investigation					2100:2112	The therapeutic investigation	2084:2112	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model	2084:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	10	126	theme	colchicine	1188:1197	arg1	nanoparticles					1141:1153	mesoporous silica nanoparticles	1123:1153	mesoporous silica nanoparticles (MSNs)	1123:1160	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	10	126	theme	colchicine	1188:1197	arg1	encapsulators					1199:1211	colchicine encapsulators	1188:1211	colchicine encapsulators	1188:1211	Cellulose-based patches were primed, where mesoporous silica nanoparticles (MSNs) were prepared and used as colchicine encapsulators.					
32693125	17	127	theme	isolated	1807:1814	arg1	skin					1820:1823	isolated rat skin	1807:1823	isolated rat skin	1807:1823	Ex vivo drug permeation study through isolated rat skin was conducted.					
32693125	3	128	theme	potential	405:413	arg1	Colchicine					362:371	Colchicine	362:371	Colchicine	362:371	Colchicine has been recently observed as a potential treatment for osteoarthritis (OA).					
32693125	3	128	theme	potential	405:413	arg1	treatment					415:423	a potential treatment	403:423	a potential treatment for osteoarthritis (OA)	403:447	Colchicine has been recently observed as a potential treatment for osteoarthritis (OA).					
32693125	18	129	theme	drug	1915:1918	arg1	flux					1920:1923	enhanced drug flux	1906:1923	enhanced drug flux	1906:1923	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32693125	20	130	theme	patches	2573:2579	arg1	potentiality					2491:2502	significant potentiality	2479:2502	significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management	2479:2664	Histopathology of rats knee joint supported the OA protective effect of the developed patches, The obtained results revealed significant potentiality of the developed colchicine mesoporous silica nanoparticles/hydrogel patches in the offering, efficient safe and patient convenient formulation for OA management.					
32693125	8	131	theme	skin	960:963	arg1	permeation					965:974	poor skin permeation	955:974	poor skin permeation	955:974	However, colchicine transdermal delivery is challenging owing to its high aqueous solubility and hence poor skin permeation.					
32693125	16	132	theme	fabric	1751:1756	arg1	stiffness					1758:1766	fabric stiffness	1751:1766	fabric stiffness	1751:1766	The prepared cotton patch was tested for fabric stiffness.					
32693125	19	133	theme	patches	2139:2145	arg1	investigation					2100:2112	The therapeutic investigation	2084:2112	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model	2084:2204	The therapeutic investigation of colchicine formulated patches in mono-iodoacetate (MIA)-induced rat osteoarthritis model depicted improved locomotor activity, glutathione blood level, and remarkable decline in levels of malondialdehyde, nitric oxide, TNF-α, and COX-2.					
32693125	18	134	theme	permeated	1939:1947	arg1	levels					1954:1959	permeated drug levels	1939:1959	permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein	1939:2081	In comparison to free drug aqueous solution, the patches revealed enhanced drug flux and amplified permeated drug levels which were sustained all over 24 h. Skin permeation was further validated via confocal laser microscopy using fluorescein.					
32718594	3	0	theme	kPa-1	459:463	arg1	h-1m-2					465:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	0	theme	kPa-1	459:463	arg1	moisture					428:435	moisture	428:435	moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2)	428:471	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	0	1	theme	leathers	76:83	arg1	production					40:49	the production	36:49	the production of cellulose-aided peach leathers	36:83	Escalating the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant.					
32718594	5	2	theme	bench	818:822	arg1	casting					824:830	bench casting	818:830	bench casting	818:830	The laboratory-scale leather production via bench casting was extended to a pilot-scale through continuous casting.					
32718594	3	3	theme	plasticizing	598:609	arg1	effect					611:616	the plasticizing effect	594:616	the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF	594:675	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	6	4	theme	up	912:913	arg1	effect					894:899	The effect	890:899	The effect of scaling up on the nutritional and sensory features of the peach leather	890:974	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32718594	7	5	theme	packaging	1158:1166	arg1	production					1101:1110	the large-scale production	1085:1110	the large-scale production of peach leather towards applications	1085:1148	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	7	5	theme	packaging	1158:1166	arg1	materials					1168:1176	packaging materials	1158:1176	packaging materials	1158:1176	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	3	6	dep	5.6	450:452	arg1	to					447:448	to	447:448	to	447:448	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	2	7	theme	peach	267:271	arg1	pulp					273:276	peach pulp	267:276	peach pulp	267:276	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	6	8	theme	scaling	904:910	arg1	up					912:913	scaling up	904:913	scaling up	904:913	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32718594	3	9	theme	HPMC	635:638	arg1	matrix					640:645	HPMC matrix	635:645	HPMC matrix	635:645	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	7	10	theme	peach	1115:1119	arg1	leather					1121:1127	peach leather	1115:1127	peach leather	1115:1127	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	6	11	theme	peach	962:966	arg1	leather					968:974	the peach leather	958:974	the peach leather	958:974	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32718594	5	12	theme	laboratory-scale	778:793	arg1	production					803:812	The laboratory-scale leather production	774:812	The laboratory-scale leather production via bench casting	774:830	The laboratory-scale leather production via bench casting was extended to a pilot-scale through continuous casting.					
32718594	3	13	theme	peach	621:625	arg1	pulp					627:630	peach pulp	621:630	peach pulp	621:630	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	14	theme	Increased	402:410	arg1	permeability					412:423	Increased permeability	402:423	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa)	402:582	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	7	15	theme	leather	1121:1127	arg1	production					1101:1110	the large-scale production	1085:1110	the large-scale production of peach leather towards applications	1085:1148	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	7	15	theme	leather	1121:1127	arg1	materials					1168:1176	packaging materials	1158:1176	packaging materials	1158:1176	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	3	16	theme	pulp	627:630	arg1	effect					611:616	the plasticizing effect	594:616	the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF	594:675	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	2	17	theme	cellulose	355:363	arg1	micro/nanofibrils					365:381	cellulose micro/nanofibrils	355:381	cellulose micro/nanofibrils (CMNF)	355:388	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	2	17	theme	cellulose	355:363	arg1	CMNF					384:387	CMNF	384:387	CMNF	384:387	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	4	18	theme	leather	753:759	arg1	composition					761:771	leather composition	753:771	leather composition	753:771	A ternary mixture design allowed building response surfaces and optimizing leather composition.					
32718594	7	19	theme	established	1006:1016	arg1	useful					1067:1072	useful	1067:1072	useful	1067:1072	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	7	19	theme	established	1006:1016	arg1	correlations					1050:1061	The herein established composition-processing-property correlations	995:1061	The herein established composition-processing-property correlations	995:1061	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	2	20	dep	leathers	241:248	arg1	combined					283:290	combined	283:290	combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers	283:399	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	2	20	dep	leathers	241:248	arg1	based					250:254	based	250:254	based chiefly on peach pulp	250:276	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	0	21	theme	pilot	111:115	arg1	plant					117:121	the pilot plant	107:121	the pilot plant	107:121	Escalating the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant.					
32718594	4	22	theme	response	720:727	arg1	surfaces					729:736	response surfaces	720:736	response surfaces	720:736	A ternary mixture design allowed building response surfaces and optimizing leather composition.					
32718594	5	23	theme	continuous	870:879	arg1	casting					881:887	continuous casting	870:887	continuous casting	870:887	The laboratory-scale leather production via bench casting was extended to a pilot-scale through continuous casting.					
32718594	7	24	theme	composition-processing-property	1018:1048	arg1	useful					1067:1072	useful	1067:1072	useful	1067:1072	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	7	24	theme	composition-processing-property	1018:1048	arg1	correlations					1050:1061	The herein established composition-processing-property correlations	995:1061	The herein established composition-processing-property correlations	995:1061	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	1	25	theme	functional	182:191	arg1	materials					193:201	sustainable functional materials	170:201	sustainable functional materials	170:201	This contribution falls within the context of sustainable functional materials.					
32718594	0	26	theme	technical	15:23	arg1	bounds					25:30	the technical bounds	11:30	the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant	11:121	Escalating the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant.					
32718594	1	27	theme	materials	193:201	arg1	context					159:165	the context	155:165	the context of sustainable functional materials	155:201	This contribution falls within the context of sustainable functional materials.					
32718594	7	28	theme	large-scale	1089:1099	arg1	production					1101:1110	the large-scale production	1085:1110	the large-scale production of peach leather towards applications	1085:1148	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	7	28	theme	large-scale	1089:1099	arg1	materials					1168:1176	packaging materials	1158:1176	packaging materials	1158:1176	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	3	29	theme	mechanical	520:529	arg1	resistance					531:540	reduced mechanical resistance	512:540	reduced mechanical resistance	512:540	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	1	30	theme	sustainable	170:180	arg1	materials					193:201	sustainable functional materials	170:201	sustainable functional materials	170:201	This contribution falls within the context of sustainable functional materials.					
32718594	6	31	from	effect	894:899	arg1	features					946:953	the nutritional and sensory features	918:953	the nutritional and sensory features of the peach leather	918:974	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32718594	3	32	dep	17	503:504	arg1	to					500:501	to	500:501	to	500:501	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	33	from	effect	611:616	arg1	matrix					640:645	HPMC matrix	635:645	HPMC matrix	635:645	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	7	34	theme	nutritional	1185:1195	arg1	leathers					1197:1204	nutritional leathers	1185:1204	nutritional leathers	1185:1204	The herein established composition-processing-property correlations are useful to support the large-scale production of peach leather towards applications both as packaging materials and as nutritional leathers.					
32718594	3	35	theme	g	454:454	arg1	h-1m-2					465:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	35	theme	g	454:454	arg1	moisture					428:435	moisture	428:435	moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2)	428:471	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	36	theme	reduced	512:518	arg1	resistance					531:540	reduced mechanical resistance	512:540	reduced mechanical resistance	512:540	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	37	theme	mm	456:457	arg1	h-1m-2					465:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	37	theme	mm	456:457	arg1	moisture					428:435	moisture	428:435	moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2)	428:471	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	4	38	theme	mixture	688:694	arg1	design					696:701	A ternary mixture design	678:701	A ternary mixture design	678:701	A ternary mixture design allowed building response surfaces and optimizing leather composition.					
32718594	0	39	from	benchtop	95:102	arg1	bounds					25:30	the technical bounds	11:30	the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant	11:121	Escalating the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant.					
32718594	4	40	theme	ternary	680:686	arg1	design					696:701	A ternary mixture design	678:701	A ternary mixture design	678:701	A ternary mixture design allowed building response surfaces and optimizing leather composition.					
32718594	5	41	theme	leather	795:801	arg1	production					803:812	The laboratory-scale leather production	774:812	The laboratory-scale leather production via bench casting	774:830	The laboratory-scale leather production via bench casting was extended to a pilot-scale through continuous casting.					
32718594	3	42	theme	GPa-18	572:577	arg1	MPa					579:581	1.8 GPa-18 MPa	568:581	1.8 GPa-18 MPa	568:581	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	42	theme	GPa-18	572:577	arg1	extensibility					477:489	extensibility	477:489	extensibility (from 10 to 17%)	477:506	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	2	43	theme	leathers	241:248	arg1	production					221:230	the production	217:230	the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers	217:399	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	2	44	theme	hydroxypropyl	297:309	arg1	HPMC					328:331	HPMC	328:331	HPMC	328:331	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	2	44	theme	hydroxypropyl	297:309	arg1	methylcellulose					311:325	hydroxypropyl methylcellulose	297:325	hydroxypropyl methylcellulose (HPMC)	297:332	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	3	45	from	%	505:505	arg1	h-1m-2					465:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	from 0.9 to 5.6 g mm kPa-1 h-1m-2	438:470	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	45	from	%	505:505	arg1	resistance					531:540	reduced mechanical resistance	512:540	reduced mechanical resistance	512:540	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	45	from	%	505:505	arg1	stiffness					557:565	stiffness	557:565	stiffness	557:565	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	45	from	%	505:505	arg1	extensibility					477:489	extensibility	477:489	extensibility (from 10 to 17%)	477:506	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	45	from	%	505:505	arg1	MPa					548:550	67-2 MPa	543:550	67-2 MPa	543:550	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	45	from	%	505:505	arg1	moisture					428:435	moisture	428:435	moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2)	428:471	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	45	from	%	505:505	arg1	MPa					579:581	1.8 GPa-18 MPa	568:581	1.8 GPa-18 MPa	568:581	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	6	46	theme	sensory	938:944	arg1	features					946:953	the nutritional and sensory features	918:953	the nutritional and sensory features of the peach leather	918:974	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32718594	0	47	theme	peach	70:74	arg1	leathers					76:83	cellulose-aided peach leathers	54:83	cellulose-aided peach leathers	54:83	Escalating the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant.					
32718594	2	48	theme	fruit	235:239	arg1	leathers					241:248	fruit leathers	235:248	fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers	235:399	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	6	49	theme	leather	968:974	arg1	features					946:953	the nutritional and sensory features	918:953	the nutritional and sensory features of the peach leather	918:974	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32718594	0	50	theme	cellulose-aided	54:68	arg1	leathers					76:83	cellulose-aided peach leathers	54:83	cellulose-aided peach leathers	54:83	Escalating the technical bounds for the production of cellulose-aided peach leathers: From the benchtop to the pilot plant.					
32718594	3	51	theme	67-2	543:546	arg1	extensibility					477:489	extensibility	477:489	extensibility (from 10 to 17%)	477:506	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	3	51	theme	67-2	543:546	arg1	MPa					548:550	67-2 MPa	543:550	67-2 MPa	543:550	Increased permeability to moisture (from 0.9 to 5.6 g mm kPa-1 h-1m-2) and extensibility (from 10 to 17%) but reduced mechanical resistance (67-2 MPa) and stiffness (1.8 GPa-18 MPa) evidenced the plasticizing effect of peach pulp in HPMC matrix, which was reinforced by CMNF.					
32718594	2	52	theme	binding	337:343	arg1	agent					345:349	binding agent	337:349	binding agent	337:349	We report on the production of fruit leathers based chiefly on peach pulp, but combined with hydroxypropyl methylcellulose (HPMC) as binding agent and cellulose micro/nanofibrils (CMNF) as fillers.					
32718594	6	53	theme	nutritional	922:932	arg1	features					946:953	the nutritional and sensory features	918:953	the nutritional and sensory features of the peach leather	918:974	The effect of scaling up on the nutritional and sensory features of the peach leather was also depicted.					
32864721	0	0	theme	Water-Soluble	37:49	arg1	Drug					51:54	Poorly Water-Soluble Drug	30:54	Poorly Water-Soluble Drug	30:54	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	6	1	theme	CMC	1115:1117	arg1	concentration					1119:1131	the CMC concentration	1111:1131	the CMC concentration of the film	1111:1143	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	3	2	theme	CMC	624:626	arg1	NPs					636:638	CMC and ZnO NPs	624:638	CMC and ZnO NPs	624:638	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	9	3	theme	passive	1401:1407	arg1	diffusion					1409:1417	passive diffusion	1401:1417	passive diffusion of the drug from the film with the absence of the electric field	1401:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	10	4	theme	study	1516:1520	arg1	outcome					1500:1506	The outcome	1496:1506	The outcome of this study	1496:1520	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	5	5	dep	PLA	827:829	arg1	NPs					839:841	NPs	839:841	NPs	839:841	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	10	6	theme	electric-responsive	1547:1565	arg1	platform					1581:1588	an electric-responsive nanocomposite platform	1544:1588	an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs	1544:1645	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	9	7	theme	drug	1426:1429	arg1	diffusion					1409:1417	passive diffusion	1401:1417	passive diffusion of the drug from the film with the absence of the electric field	1401:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	6	8	theme	deionized	991:999	arg1	water					1001:1005	deionized water	991:1005	deionized water	991:1005	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	3	9	theme	ZnO	632:634	arg1	NPs					636:638	CMC and ZnO NPs	624:638	CMC and ZnO NPs	624:638	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	1	10	dep	they	246:249	arg1	exploit					251:257	exploit	251:257	exploit less complex	251:270	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	10	11	theme	platform	1581:1588	arg1	design					1534:1539	the design	1530:1539	the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs	1530:1645	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	3	12	theme	PLA/curcumin	655:666	arg1	medium					668:673	solubilized PLA/curcumin medium	643:673	solubilized PLA/curcumin medium	643:673	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	3	13	theme	casting	696:702	arg1	step					704:707	solvent casting step	688:707	solvent casting step	688:707	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	1	14	theme	various	141:147	arg1	DDS					211:213	electric-responsive DDS	191:213	electric-responsive DDS	191:213	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	1	14	theme	various	141:147	arg1	types					149:153	various types	141:153	various types of external stimuli-responsive DDS	141:188	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	6	15	theme	RESULTS	1041:1047	arg1	field					1025:1029	DC electric field	1013:1029	DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film	1013:1143	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	8	16	theme	field	1298:1302	arg1	application					1270:1280	the application	1266:1280	the application of the electric field	1266:1302	In response to the application of the electric field, the composite films released drug with a near-linear profile.					
32864721	7	17	theme	tensile	1219:1225	arg1	properties					1227:1236	the tensile properties	1215:1236	the tensile properties of the film	1215:1248	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	9	18	theme	field	1478:1482	arg1	absence					1454:1460	the absence	1450:1460	the absence of the electric field	1450:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	3	19	from	dispersion	610:619	arg1	medium					668:673	solubilized PLA/curcumin medium	643:673	solubilized PLA/curcumin medium	643:673	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	1	20	theme	stimuli-responsive	167:184	arg1	DDS					186:188	external stimuli-responsive DDS	158:188	external stimuli-responsive DDS	158:188	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	6	21	theme	electric	1016:1023	arg1	field					1025:1029	DC electric field	1013:1029	DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film	1013:1143	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	0	22	theme	/Carboxymethyl	78:91	arg1	Film					121:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	2	23	theme	biocompatible	426:438	arg1	biopolymers					440:450	intrinsically biocompatible biopolymers	412:450	intrinsically biocompatible biopolymers	412:450	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	1	24	theme	miniaturized	280:291	arg1	generators					309:318	miniaturized electric signal generators	280:318	miniaturized electric signal generators	280:318	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	0	25	theme	Drug	51:54	arg1	Release					19:25	Release	19:25	Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	19:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	7	26	theme	NPs	1184:1186	arg1	%					1190:1190	2%	1189:1190	2%	1189:1190	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	7	26	theme	NPs	1184:1186	arg1	amount					1170:1175	a small amount	1162:1175	a small amount of ZnO NPs (2%)	1162:1191	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	7	26	theme	NPs	1184:1186	arg1	NPs					1184:1186	ZnO NPs	1180:1186	ZnO NPs (2%)	1180:1191	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	1	27	theme	electric	293:300	arg1	generators					309:318	miniaturized electric signal generators	280:318	miniaturized electric signal generators	280:318	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	6	28	theme	films	1079:1083	arg1	conductivity					1059:1070	The ionic conductivity	1049:1070	The ionic conductivity of the films	1049:1083	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	2	29	theme	drug	474:477	arg1	delivery					479:486	electro-simulative drug delivery	455:486	electro-simulative drug delivery	455:486	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	5	30	theme	FTIR	868:871	arg1	spectroscopy					884:895	FTIR, impedance spectroscopy	868:895	spectroscopy	884:895	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	2	31	theme	drug	539:542	arg1	release					503:509	the release	499:509	the release of poorly-soluble/non-ionic drug	499:542	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	1	32	theme	DDS	186:188	arg1	DDS					211:213	electric-responsive DDS	191:213	electric-responsive DDS	191:213	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	1	32	theme	DDS	186:188	arg1	types					149:153	various types	141:153	various types of external stimuli-responsive DDS	141:188	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	7	33	theme	small	1164:1168	arg1	%					1190:1190	2%	1189:1190	2%	1189:1190	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	7	33	theme	small	1164:1168	arg1	amount					1170:1175	a small amount	1162:1175	a small amount of ZnO NPs (2%)	1162:1191	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	7	33	theme	small	1164:1168	arg1	NPs					1184:1186	ZnO NPs	1180:1186	ZnO NPs (2%)	1180:1191	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	9	34	from	film	1440:1443	arg1	amount					1391:1396	no noticeable amount	1377:1396	no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field	1377:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	9	34	from	film	1440:1443	arg1	diffusion					1409:1417	passive diffusion	1401:1417	passive diffusion of the drug from the film with the absence of the electric field	1401:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	6	35	theme	ionic	1053:1057	arg1	conductivity					1059:1070	The ionic conductivity	1049:1070	The ionic conductivity of the films	1049:1083	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	3	36	theme	CMC/PLA/ZnO/CUR	553:567	arg1	films					583:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films	545:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films	545:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	9	37	from	amount	1391:1396	arg1	film					1440:1443	the film	1436:1443	the film	1436:1443	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	11	38	dep	abstract	1658:1665	arg1	Graphical					1648:1656	Graphical	1648:1656	Graphical	1648:1656	Graphical abstract.					
32864721	9	39	with	diffusion	1409:1417	arg1	absence					1454:1460	the absence	1450:1460	the absence of the electric field	1450:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	5	40	dep	spectroscopy	884:895	arg1	impedance					874:882	FTIR, impedance spectroscopy	868:895	impedance	874:882	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	2	41	theme	biopolymers	440:450	arg1	use					405:407	the use	401:407	the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery	401:486	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	5	42	theme	ZnO	835:837	arg1	contents					810:817	different contents	800:817	different contents of CMC, PLA and ZnO NPs	800:841	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	7	43	theme	film	1245:1248	arg1	properties					1227:1236	the tensile properties	1215:1236	the tensile properties of the film	1215:1248	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	9	44	theme	noticeable	1380:1389	arg1	amount					1391:1396	no noticeable amount	1377:1396	no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field	1377:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	9	44	theme	noticeable	1380:1389	arg1	diffusion					1409:1417	passive diffusion	1401:1417	passive diffusion of the drug from the film with the absence of the electric field	1401:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	6	45	theme	drug	946:949	arg1	release					951:957	The in vitro drug release	933:957	The in vitro drug release of the films	933:970	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	5	46	theme	different	800:808	arg1	contents					810:817	different contents	800:817	different contents of CMC, PLA and ZnO NPs	800:841	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	6	47	theme	in	937:938	arg1	release					951:957	The in vitro drug release	933:957	The in vitro drug release of the films	933:970	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	9	48	theme	diffusion	1409:1417	arg1	amount					1391:1396	no noticeable amount	1377:1396	no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field	1377:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	9	48	theme	diffusion	1409:1417	arg1	diffusion					1409:1417	passive diffusion	1401:1417	passive diffusion of the drug from the film with the absence of the electric field	1401:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	10	49	theme	nanocomposite	1567:1579	arg1	platform					1581:1588	an electric-responsive nanocomposite platform	1544:1588	an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs	1544:1645	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	3	50	theme	NPs	636:638	arg1	dispersion					610:619	the dispersion	606:619	the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium	606:673	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	1	51	theme	electric-responsive	191:209	arg1	DDS					211:213	electric-responsive DDS	191:213	electric-responsive DDS	191:213	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	1	51	theme	electric-responsive	191:209	arg1	types					149:153	various types	141:153	various types of external stimuli-responsive DDS	141:188	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	5	52	theme	FESEM	918:922	arg1	imaging					924:930	FESEM imaging	918:930	FESEM imaging	918:930	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	1	53	theme	promising	224:232	arg1	PURPOSE					127:133	PURPOSE	127:133	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS	127:213	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	1	53	theme	promising	224:232	arg1	carriers					234:241	more promising carriers	219:241	more promising carriers	219:241	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	3	54	theme	solubilized	643:653	arg1	medium					668:673	solubilized PLA/curcumin medium	643:673	solubilized PLA/curcumin medium	643:673	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	4	55	used	used	747:750	arg2	drug					765:768	the model drug	755:768	the model drug in this study	755:782	Curcumin is poorly water-soluble and used as the model drug in this study.					
32864721	4	55	used	used	747:750	arg2	Curcumin					710:717	Curcumin	710:717	Curcumin	710:717	Curcumin is poorly water-soluble and used as the model drug in this study.					
32864721	6	56	theme	films	966:970	arg1	release					951:957	The in vitro drug release	933:957	The in vitro drug release of the films	933:970	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	0	57	from	Film	121:124	arg1	Release					19:25	Release	19:25	Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	19:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	5	58	with	films	789:793	arg1	contents					810:817	different contents	800:817	different contents of CMC, PLA and ZnO NPs	800:841	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	3	59	theme	solvent	688:694	arg1	step					704:707	solvent casting step	688:707	solvent casting step	688:707	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	0	60	theme	Lactic	66:71	arg1	Acid					73:76	Lactic Acid	66:76	Lactic Acid	66:76	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	0	60	theme	Lactic	66:71	arg1	Poly					61:64	Poly	61:64	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	9	61	theme	electric	1469:1476	arg1	field					1478:1482	the electric field	1465:1482	the electric field	1465:1482	There was no noticeable amount of passive diffusion of the drug from the film with the absence of the electric field.					
32864721	6	62	theme	4.5 V.	1034:1039	arg1	RESULTS					1041:1047	4.5 V. RESULTS	1034:1047	4.5 V. RESULTS	1034:1047	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	8	63	theme	electric	1289:1296	arg1	field					1298:1302	the electric field	1285:1302	the electric field	1285:1302	In response to the application of the electric field, the composite films released drug with a near-linear profile.					
32864721	10	64	dep	CONCLUSION	1485:1494	arg1	enabled					1522:1528	enabled	1522:1528	enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs	1522:1645	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	1	65	theme	electric	359:366	arg1	signals					368:374	the electric signals	355:374	the electric signals	355:374	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	1	66	theme	external	158:165	arg1	DDS					186:188	external stimuli-responsive DDS	158:188	external stimuli-responsive DDS	158:188	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	10	67	theme	drugs	1641:1645	arg1	delivery					1598:1605	the delivery	1594:1605	the delivery of poorly water-soluble/non-ionic drugs	1594:1645	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	6	68	dep	field	1025:1029	arg1	increased					1085:1093	increased	1085:1093	increased with increasing the CMC concentration of the film	1085:1143	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	4	69	theme	model	759:763	arg1	Curcumin					710:717	Curcumin	710:717	Curcumin	710:717	Curcumin is poorly water-soluble and used as the model drug in this study.					
32864721	4	69	theme	model	759:763	arg1	drug					765:768	the model drug	755:768	the model drug in this study	755:782	Curcumin is poorly water-soluble and used as the model drug in this study.					
32864721	0	70	theme	Cellulose/ZnO	93:105	arg1	Film					121:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	6	71	theme	DC	1013:1014	arg1	field					1025:1029	DC electric field	1013:1029	DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film	1013:1143	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	8	72	theme	composite	1309:1317	arg1	films					1319:1323	the composite films	1305:1323	the composite films	1305:1323	In response to the application of the electric field, the composite films released drug with a near-linear profile.					
32864721	5	73	theme	tensile	898:904	arg1	testing					906:912	tensile testing	898:912	tensile testing	898:912	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	10	74	theme	water-soluble/non-ionic	1617:1639	arg1	drugs					1641:1645	poorly water-soluble/non-ionic drugs	1610:1645	poorly water-soluble/non-ionic drugs	1610:1645	CONCLUSION The outcome of this study enabled the design of an electric-responsive nanocomposite platform for the delivery of poorly water-soluble/non-ionic drugs.					
32864721	7	75	theme	ZnO	1180:1182	arg1	%					1190:1190	2%	1189:1190	2%	1189:1190	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	7	75	theme	ZnO	1180:1182	arg1	NPs					1184:1186	ZnO NPs	1180:1186	ZnO NPs (2%)	1180:1191	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	7	76	theme	amount	1170:1175	arg1	addition					1150:1157	The addition	1146:1157	The addition of a small amount of ZnO NPs (2%)	1146:1191	The addition of a small amount of ZnO NPs (2%) successfully restored the tensile properties of the film.					
32864721	1	77	theme	signal	302:307	arg1	generators					309:318	miniaturized electric signal generators	280:318	miniaturized electric signal generators	280:318	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	3	78	dep	prepared	594:601	arg1	followed					676:683	followed	676:683	followed by solvent casting step	676:707	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	8	79	theme	near-linear	1346:1356	arg1	profile					1358:1364	a near-linear profile	1344:1364	a near-linear profile	1344:1364	In response to the application of the electric field, the composite films released drug with a near-linear profile.					
32864721	2	80	theme	electro-simulative	455:472	arg1	delivery					479:486	electro-simulative drug delivery	455:486	electro-simulative drug delivery	455:486	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	5	81	theme	PLA	827:829	arg1	contents					810:817	different contents	800:817	different contents of CMC, PLA and ZnO NPs	800:841	The films with different contents of CMC, PLA and ZnO NPs were characterized using FTIR, impedance spectroscopy, tensile testing and FESEM imaging.					
32864721	2	82	theme	poorly-soluble/non-ionic	514:537	arg1	drug					539:542	poorly-soluble/non-ionic drug	514:542	poorly-soluble/non-ionic drug	514:542	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	0	83	theme	Nanocomposite	107:119	arg1	Film					121:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	0	84	theme	Poly	61:64	arg1	Film					121:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film	61:124	Electro-Stimulated Release of Poorly Water-Soluble Drug from Poly(Lactic Acid)/Carboxymethyl Cellulose/ZnO Nanocomposite Film.					
32864721	2	85	from	use	405:407	arg1	delivery					479:486	electro-simulative drug delivery	455:486	electro-simulative drug delivery	455:486	This study investigates the use of intrinsically biocompatible biopolymers in electro-simulative drug delivery to enhance the release of poorly-soluble/non-ionic drug.					
32864721	3	86	theme	nanocomposite	569:581	arg1	films					583:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films	545:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films	545:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	6	87	theme	film	1140:1143	arg1	concentration					1119:1131	the CMC concentration	1111:1131	the CMC concentration of the film	1111:1143	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	1	88	dep	carriers	234:241	arg1	generators					309:318	miniaturized electric signal generators	280:318	miniaturized electric signal generators	280:318	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	1	88	dep	carriers	234:241	arg1	possibility					328:338	the possibility	324:338	the possibility of fine-tuning the electric signals	324:374	PURPOSE Among various types of external stimuli-responsive DDS, electric-responsive DDS are more promising carriers as they exploit less complex, easily miniaturized electric signal generators and the possibility of fine-tuning the electric signals.					
32864721	3	89	theme	METHODS	545:551	arg1	films					583:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films	545:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films	545:587	METHODS CMC/PLA/ZnO/CUR nanocomposite films were prepared by the dispersion of CMC and ZnO NPs in solubilized PLA/curcumin medium, followed by solvent casting step.					
32864721	8	90	dep	application	1270:1280	arg1	response					1254:1261	response	1254:1261	response	1254:1261	In response to the application of the electric field, the composite films released drug with a near-linear profile.					
32864721	6	91	dep	in	937:938	arg1	vitro					940:944	vitro	940:944	vitro	940:944	The in vitro drug release of the films was carried out in deionized water under DC electric field of 4.5 V. RESULTS The ionic conductivity of the films increased with increasing the CMC concentration of the film.					
32864721	4	92	from	drug	765:768	arg1	study					778:782	this study	773:782	this study	773:782	Curcumin is poorly water-soluble and used as the model drug in this study.					
32188479	0	0	theme	infection	105:113	arg1	prevention					61:70	the prevention	57:70	the prevention of mesh-associated surgical site infection	57:113	Nylon-6/chitosan core/shell antimicrobial nanofibers for the prevention of mesh-associated surgical site infection.					
32188479	1	1	theme	hernia	174:179	arg1	repair					181:186	hernia repair	174:186	hernia repair	174:186	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	0	2	theme	site	100:103	arg1	infection					105:113	mesh-associated surgical site infection	75:113	mesh-associated surgical site infection	75:113	Nylon-6/chitosan core/shell antimicrobial nanofibers for the prevention of mesh-associated surgical site infection.					
32188479	7	3	theme	nanofibrous	1199:1209	arg1	meshes					1211:1216	The composite nanofibrous meshes	1185:1216	The composite nanofibrous meshes	1185:1216	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	8	4	theme	antimicrobial	1340:1352	arg1	effectiveness					1354:1366	The antimicrobial effectiveness	1336:1366	The antimicrobial effectiveness	1336:1366	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	2	5	theme	major	401:405	arg1	relevance					416:424	major clinical relevance	401:424	major clinical relevance for patients undergoing hernia repair	401:462	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	8	6	theme	site	1496:1499	arg1	infections					1501:1510	surgical site infections	1487:1510	surgical site infections	1487:1510	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	4	7	theme	structured	662:671	arg1	nanofibers					673:682	Core/shell structured nanofibers	651:682	Core/shell structured nanofibers	651:682	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	7	8	theme	electrospun	1316:1326	arg1	meshes					1328:1333	the counterpart single-polymer electrospun meshes	1285:1333	the counterpart single-polymer electrospun meshes	1285:1333	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	6	9	with	nanofibers	1084:1093	arg1	architecture					1119:1130	a "beads-in-fiber" architecture	1100:1130	a "beads-in-fiber" architecture	1100:1130	Homogeneous nanofibers with a "beads-in-fiber" architecture were observed by TEM, and validated by FTIR and XPS.					
32188479	7	10	theme	composite	1189:1197	arg1	meshes					1211:1216	The composite nanofibrous meshes	1185:1216	The composite nanofibrous meshes	1185:1216	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	2	11	theme	preventing	330:339	arg1	colonization					351:362	preventing bacterial colonization	330:362	preventing bacterial colonization of implanted prosthetic meshes	330:393	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	2	12	theme	bacterial	341:349	arg1	colonization					351:362	preventing bacterial colonization	330:362	preventing bacterial colonization of implanted prosthetic meshes	330:393	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	1	13	theme	antimicrobial	290:302	arg1	properties					304:313	antimicrobial properties	290:313	antimicrobial properties	290:313	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	8	14	theme	surgical	1487:1494	arg1	infections					1501:1510	surgical site infections	1487:1510	surgical site infections	1487:1510	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	9	15	theme	antimicrobial	1628:1640	arg1	surfaces					1642:1649	active antimicrobial surfaces	1621:1649	active antimicrobial surfaces	1621:1649	This study illustrates how the tailoring of core/shell nanofibers can be of interest for the development of active antimicrobial surfaces.					
32188479	3	16	theme	release	621:627	arg1	action					643:648	dual drug release antimicrobial action	611:648	dual drug release antimicrobial action	611:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	2	17	theme	clinical	407:414	arg1	relevance					416:424	major clinical relevance	401:424	major clinical relevance for patients undergoing hernia repair	401:462	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	8	18	theme	occurring	1424:1432	arg1	bacteria					1445:1452	the most commonly occurring pathogenic bacteria	1406:1452	the most commonly occurring pathogenic bacteria	1406:1452	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	7	19	theme	counterpart	1289:1299	arg1	meshes					1328:1333	the counterpart single-polymer electrospun meshes	1285:1333	the counterpart single-polymer electrospun meshes	1285:1333	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	8	20	dep	two	1399:1401	arg1	aeruginosa					1472:1481	P. aeruginosa	1469:1481	P. aeruginosa	1469:1481	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	8	20	dep	two	1399:1401	arg1	aureus					1458:1463	S. aureus	1455:1463	S. aureus	1455:1463	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	3	21	theme	co-axial	484:491	arg1	technique					509:517	the co-axial electrospinning technique	480:517	the co-axial electrospinning technique	480:517	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	7	22	theme	single-polymer	1301:1314	arg1	meshes					1328:1333	the counterpart single-polymer electrospun meshes	1285:1333	the counterpart single-polymer electrospun meshes	1285:1333	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	5	23	theme	Poly	1020:1023	arg1	release					1009:1015	the sustained release	995:1015	the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers	995:1069	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	7	24	theme	stress-strain	1244:1256	arg1	responses					1258:1266	the stress-strain responses	1240:1266	the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes	1240:1333	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	3	25	theme	dual	611:614	arg1	action					643:648	dual drug release antimicrobial action	611:648	dual drug release antimicrobial action	611:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	2	26	theme	hernia	450:455	arg1	repair					457:462	hernia repair	450:462	hernia repair	450:462	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	5	27	theme	binary	903:908	arg1	system					924:929	a binary antimicrobial system	901:929	a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell	901:987	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	5	28	theme	core/shell	867:876	arg1	structure					878:886	The core/shell structure	863:886	The core/shell structure	863:886	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	4	29	from	Nylon-6	714:720	arg1	core					729:732	the core	725:732	the core	725:732	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	2	30	theme	implanted	367:375	arg1	meshes					388:393	implanted prosthetic meshes	367:393	implanted prosthetic meshes	367:393	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	4	31	theme	appropriate	750:760	arg1	stability					773:781	the appropriate mechanical stability	746:781	the appropriate mechanical stability	746:781	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	5	32	theme	antimicrobial	910:922	arg1	system					924:929	a binary antimicrobial system	901:929	a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell	901:987	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	6	33	theme	Homogeneous	1072:1082	arg1	nanofibers					1084:1093	Homogeneous nanofibers	1072:1093	Homogeneous nanofibers with a "beads-in-fiber" architecture	1072:1130	Homogeneous nanofibers with a "beads-in-fiber" architecture were observed by TEM, and validated by FTIR and XPS.					
32188479	1	34	theme	polymeric	207:215	arg1	constructs					231:240	predominantly polymeric textile-based constructs	193:240	predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties	193:313	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	1	34	theme	polymeric	207:215	arg1	meshes					144:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	8	35	theme	pathogenic	1434:1443	arg1	bacteria					1445:1452	the most commonly occurring pathogenic bacteria	1406:1452	the most commonly occurring pathogenic bacteria	1406:1452	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	1	36	theme	state-of-the-art	120:135	arg1	constructs					231:240	predominantly polymeric textile-based constructs	193:240	predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties	193:313	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	1	36	theme	state-of-the-art	120:135	arg1	meshes					144:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	1	37	theme	textile-based	217:229	arg1	constructs					231:240	predominantly polymeric textile-based constructs	193:240	predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties	193:313	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	1	37	theme	textile-based	217:229	arg1	meshes					144:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	0	38	theme	antimicrobial	28:40	arg1	nanofibers					42:51	antimicrobial nanofibers	28:51	antimicrobial nanofibers for the prevention of mesh-associated surgical site infection	28:113	Nylon-6/chitosan core/shell antimicrobial nanofibers for the prevention of mesh-associated surgical site infection.					
32188479	4	39	theme	Core/shell	651:660	arg1	nanofibers					673:682	Core/shell structured nanofibers	651:682	Core/shell structured nanofibers	651:682	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	9	40	theme	active	1621:1626	arg1	surfaces					1642:1649	active antimicrobial surfaces	1621:1649	active antimicrobial surfaces	1621:1649	This study illustrates how the tailoring of core/shell nanofibers can be of interest for the development of active antimicrobial surfaces.					
32188479	1	41	theme	hernia	137:142	arg1	constructs					231:240	predominantly polymeric textile-based constructs	193:240	predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties	193:313	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	1	41	theme	hernia	137:142	arg1	meshes					144:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes	116:149	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	5	42	theme	sustained	999:1007	arg1	release					1009:1015	the sustained release	995:1015	the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers	995:1069	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	3	43	theme	novel	561:565	arg1	structure					587:595	a novel mechanically stable structure	559:595	a novel mechanically stable structure incorporating dual drug release antimicrobial action	559:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	9	44	theme	core/shell	1557:1566	arg1	nanofibers					1568:1577	core/shell nanofibers	1557:1577	core/shell nanofibers	1557:1577	This study illustrates how the tailoring of core/shell nanofibers can be of interest for the development of active antimicrobial surfaces.					
32188479	4	45	theme	bacteriostatic	840:853	arg1	action					855:860	bacteriostatic action	840:860	bacteriostatic action	840:860	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	9	46	theme	nanofibers	1568:1577	arg1	tailoring					1544:1552	the tailoring	1540:1552	the tailoring of core/shell nanofibers	1540:1577	This study illustrates how the tailoring of core/shell nanofibers can be of interest for the development of active antimicrobial surfaces.					
32188479	5	47	theme	fibers	1064:1069	arg1	core					1052:1055	the Nylon-6 core	1040:1055	the Nylon-6 core of the fibers	1040:1069	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	8	48	theme	bacteria	1445:1452	arg1	two					1399:1401	two	1399:1401	two	1399:1401	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	8	48	theme	bacteria	1445:1452	arg1	bacteria					1445:1452	the most commonly occurring pathogenic bacteria	1406:1452	the most commonly occurring pathogenic bacteria	1406:1452	The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections.					
32188479	7	49	from	responses	1258:1266	arg1	comparison					1271:1280	comparison	1271:1280	comparison to the counterpart single-polymer electrospun meshes	1271:1333	The composite nanofibrous meshes significantly advance the stress-strain responses in comparison to the counterpart single-polymer electrospun meshes.					
32188479	5	50	from	5-chloro-8-quinolinol	945:965	arg1	shell					983:987	the chitosan shell	970:987	the chitosan shell	970:987	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	4	51	theme	Chitosan/Polyethylene	788:808	arg1	oxide					810:814	Chitosan/Polyethylene oxide	788:814	Chitosan/Polyethylene oxide in the shell	788:827	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	5	52	from	core	1052:1055	arg1	release					1009:1015	the sustained release	995:1015	the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers	995:1069	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	4	53	theme	mechanical	762:771	arg1	stability					773:781	the appropriate mechanical stability	746:781	the appropriate mechanical stability	746:781	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	2	54	theme	meshes	388:393	arg1	colonization					351:362	preventing bacterial colonization	330:362	preventing bacterial colonization of implanted prosthetic meshes	330:393	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	3	55	theme	antimicrobial	629:641	arg1	action					643:648	dual drug release antimicrobial action	611:648	dual drug release antimicrobial action	611:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	1	56	theme	high	255:258	arg1	strength					271:278	high mechanical strength	255:278	high mechanical strength	255:278	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	3	57	theme	drug	616:619	arg1	action					643:648	dual drug release antimicrobial action	611:648	dual drug release antimicrobial action	611:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	2	58	theme	prosthetic	377:386	arg1	meshes					388:393	implanted prosthetic meshes	367:393	implanted prosthetic meshes	367:393	Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair.					
32188479	4	59	from	oxide	810:814	arg1	shell					823:827	the shell	819:827	the shell	819:827	Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action.					
32188479	1	60	theme	mechanical	260:269	arg1	strength					271:278	high mechanical strength	255:278	high mechanical strength	255:278	The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties.					
32188479	0	61	theme	surgical	91:98	arg1	infection					105:113	mesh-associated surgical site infection	75:113	mesh-associated surgical site infection	75:113	Nylon-6/chitosan core/shell antimicrobial nanofibers for the prevention of mesh-associated surgical site infection.					
32188479	5	62	theme	chitosan	974:981	arg1	shell					983:987	the chitosan shell	970:987	the chitosan shell	970:987	The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers.					
32188479	9	63	theme	surfaces	1642:1649	arg1	development					1606:1616	the development	1602:1616	the development of active antimicrobial surfaces	1602:1649	This study illustrates how the tailoring of core/shell nanofibers can be of interest for the development of active antimicrobial surfaces.					
32188479	3	64	theme	stable	580:585	arg1	structure					587:595	a novel mechanically stable structure	559:595	a novel mechanically stable structure incorporating dual drug release antimicrobial action	559:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	3	65	theme	electrospinning	493:507	arg1	technique					509:517	the co-axial electrospinning technique	480:517	the co-axial electrospinning technique	480:517	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	6	66	theme	"	1117:1117	arg1	architecture					1119:1130	a "beads-in-fiber" architecture	1100:1130	a "beads-in-fiber" architecture	1100:1130	Homogeneous nanofibers with a "beads-in-fiber" architecture were observed by TEM, and validated by FTIR and XPS.					
32188479	0	67	theme	mesh-associated	75:89	arg1	infection					105:113	mesh-associated surgical site infection	75:113	mesh-associated surgical site infection	75:113	Nylon-6/chitosan core/shell antimicrobial nanofibers for the prevention of mesh-associated surgical site infection.					
32188479	3	68	theme	structure	587:595	arg1	development					544:554	the development	540:554	the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action	540:648	In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action.					
32188479	6	69	theme	beads-in-fiber	1103:1116	arg1	architecture					1119:1130	a "beads-in-fiber" architecture	1100:1130	a "beads-in-fiber" architecture	1100:1130	Homogeneous nanofibers with a "beads-in-fiber" architecture were observed by TEM, and validated by FTIR and XPS.					
32338869	0	0	from	Self-Healing	21:32	arg1	Polymer					64:70	Polymer	64:70	Polymer	64:70	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	7	1	theme	volume	1100:1105	arg1	recovery					1107:1114	volume recovery	1100:1114	volume recovery	1100:1114	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	8	2	theme	humanoid	1408:1415	arg1	robotics					1417:1424	humanoid robotics	1408:1424	humanoid robotics	1408:1424	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	2	theme	humanoid	1408:1415	arg1	dressing					1355:1362	wound dressing	1349:1362	wound dressing	1349:1362	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	6	3	theme	original	946:953	arg1	Meanwhile					931:939	Meanwhile	931:939	Meanwhile	931:939	Meanwhile, its original volume can be easily recovered by subsequent contraction.					
32338869	6	3	theme	original	946:953	arg1	volume					955:960	its original volume	942:960	its original volume	942:960	Meanwhile, its original volume can be easily recovered by subsequent contraction.					
32338869	0	4	theme	Volume	86:91	arg1	Expansion					93:101	Rapid Volume Expansion	80:101	Rapid Volume Expansion	80:101	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	8	5	theme	human	1458:1462	arg1	motions					1464:1470	large-scale human motions	1446:1470	large-scale human motions	1446:1470	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	0	6	theme	Host-Guest	109:118	arg1	Interaction					120:130	Host-Guest Interaction	109:130	Host-Guest Interaction	109:130	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	4	7	theme	fast	669:672	arg1	self-repair					674:684	fast self-repair	669:684	fast self-repair of microcracks based on reversible host-guest assembly	669:739	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	7	8	theme	short-term	1165:1174	arg1	stimulus					1184:1191	the short-term cooling stimulus	1161:1191	the short-term cooling stimulus	1161:1191	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	7	9	theme	experimental	1036:1047	arg1	data					1049:1052	the experimental data	1032:1052	the experimental data	1032:1052	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	8	10	theme	good	1220:1223	arg1	biocompatibility					1225:1240	good biocompatibility	1220:1240	good biocompatibility	1220:1240	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	0	11	from	Polymer	64:70	arg1	Self-Healing					21:32	Repeatedly Intrinsic Self-Healing	0:32	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion	0:101	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	7	12	theme	cooling	1176:1182	arg1	stimulus					1184:1191	the short-term cooling stimulus	1161:1191	the short-term cooling stimulus	1161:1191	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	2	13	from	approach	382:389	arg1	work					399:402	this work	394:402	this work	394:402	To enable repeatable large-wound self-healing and volume/structure recovery, we verified a proof-of-concept approach in this work.					
32338869	2	14	theme	large-wound	295:305	arg1	self-healing					307:318	repeatable large-wound self-healing	284:318	repeatable large-wound self-healing	284:318	To enable repeatable large-wound self-healing and volume/structure recovery, we verified a proof-of-concept approach in this work.					
32338869	4	15	theme	host-guest	721:730	arg1	assembly					732:739	reversible host-guest assembly	710:739	reversible host-guest assembly	710:739	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	0	16	from	Wounds	54:59	arg1	Polymer					64:70	Polymer	64:70	Polymer	64:70	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	2	17	theme	repeatable	284:293	arg1	self-healing					307:318	repeatable large-wound self-healing	284:318	repeatable large-wound self-healing	284:318	To enable repeatable large-wound self-healing and volume/structure recovery, we verified a proof-of-concept approach in this work.					
32338869	3	18	theme	intrinsic	483:491	arg1	ability					506:512	an intrinsic self-healing ability	480:512	an intrinsic self-healing ability	480:512	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	0	19	theme	Intrinsic	11:19	arg1	Self-Healing					21:32	Repeatedly Intrinsic Self-Healing	0:32	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion	0:101	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	5	20	dep	wound	826:830	arg1	self-repair					872:882	self-repair	872:882	self-repair	872:882	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	8	21	theme	low	1199:1201	arg1	cytotoxicity					1203:1214	low cytotoxicity	1199:1214	low cytotoxicity	1199:1214	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	22	theme	large-scale	1446:1456	arg1	motions					1464:1470	large-scale human motions	1446:1470	large-scale human motions	1446:1470	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	5	23	theme	self-healing	917:928	arg1	terms					898:902	terms	898:902	terms of intrinsic self-healing	898:928	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	3	24	theme	polymer	417:423	arg1	hydrogel					425:432	a polymer hydrogel	415:432	a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers	415:624	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	8	25	theme	practical	1311:1319	arg1	self-healing					1333:1344	practical large-wound self-healing	1311:1344	practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions	1311:1470	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	5	26	theme	intrinsic	907:915	arg1	self-healing					917:928	intrinsic self-healing	907:928	intrinsic self-healing	907:928	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	1	27	theme	biological	200:209	arg1	body					211:214	a biological body	198:214	a biological body	198:214	Implantable and wearable materials, which are usually used in/on a biological body, are mostly needed with biomimetic self-healing function.					
32338869	8	28	theme	intelligent	1265:1275	arg1	hydrogel					1277:1284	this highly intelligent hydrogel	1253:1284	this highly intelligent hydrogel	1253:1284	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	29	theme	wearable	1383:1390	arg1	dressing					1355:1362	wound dressing	1349:1362	wound dressing	1349:1362	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	29	theme	wearable	1383:1390	arg1	biosensors					1392:1401	wearable biosensors	1383:1401	wearable biosensors	1383:1401	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	4	30	theme	microcracks	689:699	arg1	self-repair					674:684	fast self-repair	669:684	fast self-repair of microcracks based on reversible host-guest assembly	669:739	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	5	31	theme	large	767:771	arg1	wound					778:782	a large open wound	765:782	a large open wound	765:782	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	3	32	theme	oligomers	616:624	arg1	types					570:574	two types	566:574	two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers	566:624	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	3	33	theme	host-guest	522:531	arg1	self-assembly					544:556	host-guest orthogonal self-assembly	522:556	host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers	522:624	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	0	34	theme	Wounds	54:59	arg1	Self-Healing					21:32	Repeatedly Intrinsic Self-Healing	0:32	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion	0:101	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	8	35	theme	electronic	1365:1374	arg1	dressing					1355:1362	wound dressing	1349:1362	wound dressing	1349:1362	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	35	theme	electronic	1365:1374	arg1	skins					1376:1380	electronic skins	1365:1380	electronic skins	1365:1380	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	5	36	theme	open	773:776	arg1	wound					778:782	a large open wound	765:782	a large open wound	765:782	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	4	37	dep	thermosensitive	641:655	arg1	thermosensitive					641:655	thermosensitive	641:655	thermosensitive	641:655	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	4	37	dep	thermosensitive	641:655	arg1	result					631:636	The result	627:636	The result	627:636	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	4	37	dep	thermosensitive	641:655	arg1	capable					658:664	capable	658:664	capable	658:664	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	8	38	theme	large-wound	1321:1331	arg1	self-healing					1333:1344	practical large-wound self-healing	1311:1344	practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions	1311:1470	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	3	39	theme	self-healing	493:504	arg1	ability					506:512	an intrinsic self-healing ability	480:512	an intrinsic self-healing ability	480:512	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	0	40	theme	Millimeter-Scale	37:52	arg1	Wounds					54:59	Millimeter-Scale Wounds	37:59	Millimeter-Scale Wounds in Polymer	37:70	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	3	41	theme	temperature	448:458	arg1	responsiveness					460:473	temperature responsiveness	448:473	temperature responsiveness	448:473	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	4	42	theme	self-repair	674:684	arg1	thermosensitive					641:655	thermosensitive	641:655	thermosensitive	641:655	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	4	42	theme	self-repair	674:684	arg1	result					631:636	The result	627:636	The result	627:636	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	4	42	theme	self-repair	674:684	arg1	capable					658:664	capable	658:664	capable	658:664	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	7	43	theme	wound	1077:1081	arg1	self-healing					1083:1094	such millimeter-level wound self-healing	1055:1094	such millimeter-level wound self-healing	1055:1094	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	5	44	theme	volume	836:841	arg1	swelling					843:850	volume swelling	836:850	volume swelling	836:850	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	6	45	theme	subsequent	989:998	arg1	contraction					1000:1010	subsequent contraction	989:1010	subsequent contraction	989:1010	Meanwhile, its original volume can be easily recovered by subsequent contraction.					
32338869	1	46	theme	Implantable	133:143	arg1	materials					158:166	Implantable and wearable materials	133:166	Implantable and wearable materials	133:166	Implantable and wearable materials, which are usually used in/on a biological body, are mostly needed with biomimetic self-healing function.					
32338869	7	47	theme	such	1055:1058	arg1	self-healing					1083:1094	such millimeter-level wound self-healing	1055:1094	such millimeter-level wound self-healing	1055:1094	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	2	48	theme	proof-of-concept	365:380	arg1	approach					382:389	a proof-of-concept approach	363:389	a proof-of-concept approach in this work	363:402	To enable repeatable large-wound self-healing and volume/structure recovery, we verified a proof-of-concept approach in this work.					
32338869	5	49	from	damage	888:893	arg1	terms					898:902	terms	898:902	terms of intrinsic self-healing	898:928	More importantly, when a large open wound appears, the hydrogel can first close the wound via volume swelling and then completely self-repair the damage in terms of intrinsic self-healing.					
32338869	1	50	theme	wearable	149:156	arg1	materials					158:166	Implantable and wearable materials	133:166	Implantable and wearable materials	133:166	Implantable and wearable materials, which are usually used in/on a biological body, are mostly needed with biomimetic self-healing function.					
32338869	7	51	theme	millimeter-level	1060:1075	arg1	self-healing					1083:1094	such millimeter-level wound self-healing	1055:1094	such millimeter-level wound self-healing	1055:1094	As demonstrated by the experimental data, such millimeter-level wound self-healing and volume recovery can be repeatedly carried out in response to the short-term cooling stimulus.					
32338869	8	52	from	self-healing	1333:1344	arg1	robotics					1417:1424	humanoid robotics	1408:1424	humanoid robotics	1408:1424	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	52	from	self-healing	1333:1344	arg1	dressing					1355:1362	wound dressing	1349:1362	wound dressing	1349:1362	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	52	from	self-healing	1333:1344	arg1	skins					1376:1380	electronic skins	1365:1380	electronic skins	1365:1380	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	52	from	self-healing	1333:1344	arg1	biosensors					1392:1401	wearable biosensors	1383:1401	wearable biosensors	1383:1401	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	53	with	promising	1297:1305	arg1	cytotoxicity					1203:1214	low cytotoxicity	1199:1214	low cytotoxicity	1199:1214	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	53	with	promising	1297:1305	arg1	biocompatibility					1225:1240	good biocompatibility	1220:1240	good biocompatibility	1220:1240	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	3	54	theme	orthogonal	533:542	arg1	self-assembly					544:556	host-guest orthogonal self-assembly	522:556	host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers	522:624	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	0	55	theme	Rapid	80:84	arg1	Expansion					93:101	Rapid Volume Expansion	80:101	Rapid Volume Expansion	80:101	Repeatedly Intrinsic Self-Healing of Millimeter-Scale Wounds in Polymer through Rapid Volume Expansion Aided Host-Guest Interaction.					
32338869	8	56	theme	wound	1349:1353	arg1	robotics					1417:1424	humanoid robotics	1408:1424	humanoid robotics	1408:1424	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	56	theme	wound	1349:1353	arg1	dressing					1355:1362	wound dressing	1349:1362	wound dressing	1349:1362	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	56	theme	wound	1349:1353	arg1	skins					1376:1380	electronic skins	1365:1380	electronic skins	1365:1380	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	8	56	theme	wound	1349:1353	arg1	biosensors					1392:1401	wearable biosensors	1383:1401	wearable biosensors	1383:1401	With low cytotoxicity and good biocompatibility, moreover, this highly intelligent hydrogel is greatly promising for practical large-wound self-healing in wound dressing, electronic skins, wearable biosensors, and humanoid robotics, which can tolerate large-scale human motions.					
32338869	3	57	theme	poly	579:582	arg1	oligomers					616:624	poly(N-isopropylacrylamide) (PNIPAM) oligomers	579:624	poly(N-isopropylacrylamide) (PNIPAM) oligomers	579:624	We design a polymer hydrogel that combines temperature responsiveness with an intrinsic self-healing ability through host-guest orthogonal self-assembly between two types of poly(N-isopropylacrylamide) (PNIPAM) oligomers.					
32338869	4	58	theme	reversible	710:719	arg1	assembly					732:739	reversible host-guest assembly	710:739	reversible host-guest assembly	710:739	The result is thermosensitive, capable of fast self-repair of microcracks based on reversible host-guest assembly.					
32338869	1	59	theme	biomimetic	240:249	arg1	function					264:271	biomimetic self-healing function	240:271	biomimetic self-healing function	240:271	Implantable and wearable materials, which are usually used in/on a biological body, are mostly needed with biomimetic self-healing function.					
32338869	1	60	used	used	187:190	arg2	materials					158:166	Implantable and wearable materials	133:166	Implantable and wearable materials	133:166	Implantable and wearable materials, which are usually used in/on a biological body, are mostly needed with biomimetic self-healing function.					
32338869	1	61	theme	self-healing	251:262	arg1	function					264:271	biomimetic self-healing function	240:271	biomimetic self-healing function	240:271	Implantable and wearable materials, which are usually used in/on a biological body, are mostly needed with biomimetic self-healing function.					
33441861	0	0	theme	Shigella	79:86	arg1	sonnei					88:93	Shigella sonnei	79:93	Shigella sonnei generalized modules	79:113	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	9	1	theme	critical	1229:1236	arg1	role					1238:1241	a critical role	1227:1241	a critical role of the OAg on the immune response induced by S. sonnei GMMA	1227:1301	Our study confirms a critical role of the OAg on the immune response induced by S. sonnei GMMA.					
33441861	3	2	theme	protective	483:492	arg1	immunity					494:501	protective immunity	483:501	protective immunity	483:501	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	11	3	theme	OAg-based	1540:1548	arg1	formulations					1550:1561	other OAg-based formulations	1534:1561	other OAg-based formulations	1534:1561	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	6	4	theme	anti-OAg	932:939	arg1	drivers					966:972	the main drivers	957:972	the main drivers of bactericidal activity against OAg-positive bacteria	957:1027	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	6	4	theme	anti-OAg	932:939	arg1	antibodies					941:950	anti-OAg antibodies	932:950	anti-OAg antibodies	932:950	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	2	5	theme	vaccine	337:343	arg1	development					297:307	the development	293:307	the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis	293:425	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	0	6	theme	generalized	95:105	arg1	modules					107:113	Shigella sonnei generalized modules	79:113	Shigella sonnei generalized modules	79:113	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	0	7	theme	sonnei	88:93	arg1	modules					107:113	Shigella sonnei generalized modules	79:113	Shigella sonnei generalized modules	79:113	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	9	8	from	role	1238:1241	arg1	response					1268:1275	the immune response	1257:1275	the immune response induced by S. sonnei GMMA	1257:1301	Our study confirms a critical role of the OAg on the immune response induced by S. sonnei GMMA.					
33441861	11	9	theme	S.	1463:1464	arg1	GMMA					1473:1476	S. sonnei GMMA	1463:1476	S. sonnei GMMA	1463:1476	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	11	10	theme	protein	1443:1449	arg1	antigens					1451:1458	protein antigens	1443:1458	protein antigens	1443:1458	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	10	11	theme	OAg	1335:1337	arg1	length					1339:1344	OAg length	1335:1344	OAg length	1335:1344	However, little is known about OAg length and density regulation during infection and, therefore, protein exposure.					
33441861	9	12	theme	OAg	1250:1252	arg1	role					1238:1241	a critical role	1227:1241	a critical role of the OAg on the immune response induced by S. sonnei GMMA	1227:1301	Our study confirms a critical role of the OAg on the immune response induced by S. sonnei GMMA.					
33441861	11	13	theme	sonnei	1466:1471	arg1	GMMA					1473:1476	S. sonnei GMMA	1463:1476	S. sonnei GMMA	1463:1476	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	3	14	theme	sonnei	431:436	arg1	antigen					471:477	a key antigen	465:477	a key antigen for protective immunity	465:501	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	3	14	theme	sonnei	431:436	arg1	OAg					438:440	S. sonnei OAg	428:440	S. sonnei OAg	428:440	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	0	15	theme	modules	107:113	arg1	immunogenicity					61:74	the immunogenicity	57:74	the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA)	57:142	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	6	16	theme	antibody	901:908	arg1	binding					910:916	anti-protein antibody binding	888:916	anti-protein antibody binding	888:916	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	11	17	theme	other	1534:1538	arg1	formulations					1550:1561	other OAg-based formulations	1534:1561	other OAg-based formulations	1534:1561	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	2	18	theme	GMMA	273:276	arg1	technology					278:287	the GMMA technology	269:287	the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis	269:425	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	11	19	theme	antigens	1451:1458	arg1	presence					1431:1438	the presence	1427:1438	the presence of protein antigens on S. sonnei GMMA	1427:1476	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	6	20	theme	anti-protein	888:899	arg1	binding					910:916	anti-protein antibody binding	888:916	anti-protein antibody binding	888:916	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	3	21	theme	IgG	550:552	arg1	response					554:561	anti-OAg-specific IgG response	532:561	anti-OAg-specific IgG response	532:561	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	6	22	theme	OAg-positive	1007:1018	arg1	bacteria					1020:1027	OAg-positive bacteria	1007:1027	OAg-positive bacteria	1007:1027	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	9	23	theme	immune	1261:1266	arg1	response					1268:1275	the immune response	1257:1275	the immune response induced by S. sonnei GMMA	1257:1301	Our study confirms a critical role of the OAg on the immune response induced by S. sonnei GMMA.					
33441861	10	24	theme	density	1350:1356	arg1	regulation					1358:1367	density regulation	1350:1367	density regulation	1350:1367	However, little is known about OAg length and density regulation during infection and, therefore, protein exposure.					
33441861	3	25	theme	S.	428:429	arg1	antigen					471:477	a key antigen	465:477	a key antigen for protective immunity	465:501	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	3	25	theme	S.	428:429	arg1	OAg					438:440	S. sonnei OAg	428:440	S. sonnei OAg	428:440	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	7	26	theme	OAg-negative	1110:1121	arg1	bacteria					1123:1130	OAg-negative bacteria	1110:1130	OAg-negative bacteria	1110:1130	Interestingly, antibodies that are not targeting the OAg are functional against OAg-negative bacteria.					
33441861	11	27	theme	added	1492:1496	arg1	value					1498:1502	an added value	1489:1502	an added value for GMMA vaccines	1489:1520	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	2	28	theme	shigellosis	415:425	arg1	cause					406:410	the most epidemiologically relevant cause	370:410	the most epidemiologically relevant cause of shigellosis	370:425	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	2	28	theme	shigellosis	415:425	arg1	sonnei					362:367	Shigella sonnei	353:367	Shigella sonnei	353:367	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	6	29	theme	activity	990:997	arg1	drivers					966:972	the main drivers	957:972	the main drivers of bactericidal activity against OAg-positive bacteria	957:1027	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	6	29	theme	activity	990:997	arg1	antibodies					941:950	anti-OAg antibodies	932:950	anti-OAg antibodies	932:950	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	0	30	theme	membrane	119:126	arg1	GMMA					138:141	GMMA	138:141	GMMA	138:141	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	0	30	theme	membrane	119:126	arg1	antigens					128:135	membrane antigens	119:135	membrane antigens (GMMA)	119:142	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	1	31	attach	released	163:170	arg1	bacteria					202:209	engineered Gram-negative bacteria	177:209	engineered Gram-negative bacteria resembling the composition of outer membranes	177:255	GMMA are exosomes released from engineered Gram-negative bacteria resembling the composition of outer membranes.					
33441861	1	31	attach	released	163:170	arg2	GMMA					145:148	GMMA	145:148	GMMA	145:148	GMMA are exosomes released from engineered Gram-negative bacteria resembling the composition of outer membranes.					
33441861	3	32	theme	anti-OAg-specific	532:548	arg1	response					554:561	anti-OAg-specific IgG response	532:561	anti-OAg-specific IgG response	532:561	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	6	33	theme	bactericidal	977:988	arg1	activity					990:997	bactericidal activity	977:997	bactericidal activity against OAg-positive bacteria	977:1027	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	8	34	theme	protein	1152:1158	arg1	antigens					1160:1167	The immunodominant protein antigens	1133:1167	The immunodominant protein antigens	1133:1167	The immunodominant protein antigens were identified by proteomic analysis.					
33441861	4	35	with	vaccination	661:671	arg1	GMMA					678:681	GMMA	678:681	GMMA	678:681	The contribution of protein-specific antibodies induced upon vaccination with GMMA has never been fully elucidated.					
33441861	1	36	theme	engineered	177:186	arg1	bacteria					202:209	engineered Gram-negative bacteria	177:209	engineered Gram-negative bacteria resembling the composition of outer membranes	177:255	GMMA are exosomes released from engineered Gram-negative bacteria resembling the composition of outer membranes.					
33441861	4	37	theme	antibodies	637:646	arg1	contribution					604:615	The contribution	600:615	The contribution of protein-specific antibodies induced upon vaccination with GMMA	600:681	The contribution of protein-specific antibodies induced upon vaccination with GMMA has never been fully elucidated.					
33441861	0	38	theme	O-Antigen	31:39	arg1	contribution					15:26	the contribution	11:26	the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA)	11:142	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	11	39	from	presence	1431:1438	arg1	GMMA					1473:1476	S. sonnei GMMA	1463:1476	S. sonnei GMMA	1463:1476	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	5	40	theme	Anti-protein	716:727	arg1	antibodies					729:738	Anti-protein antibodies	716:738	Anti-protein antibodies	716:738	Anti-protein antibodies are induced in mice upon immunization with either OAg-negative and OAg-positive GMMA.					
33441861	9	41	theme	S.	1288:1289	arg1	GMMA					1298:1301	S. sonnei GMMA	1288:1301	S. sonnei GMMA	1288:1301	Our study confirms a critical role of the OAg on the immune response induced by S. sonnei GMMA.					
33441861	2	42	theme	relevant	397:404	arg1	cause					406:410	the most epidemiologically relevant cause	370:410	the most epidemiologically relevant cause of shigellosis	370:425	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	2	42	theme	relevant	397:404	arg1	sonnei					362:367	Shigella sonnei	353:367	Shigella sonnei	353:367	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	5	43	theme	OAg-positive	807:818	arg1	GMMA					820:823	either OAg-negative and OAg-positive GMMA	783:823	either OAg-negative and OAg-positive GMMA	783:823	Anti-protein antibodies are induced in mice upon immunization with either OAg-negative and OAg-positive GMMA.					
33441861	9	44	theme	sonnei	1291:1296	arg1	GMMA					1298:1301	S. sonnei GMMA	1288:1301	S. sonnei GMMA	1288:1301	Our study confirms a critical role of the OAg on the immune response induced by S. sonnei GMMA.					
33441861	11	45	attach	presence	1431:1438	arg2	antigens					1451:1458	protein antigens	1443:1458	protein antigens	1443:1458	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	11	45	attach	presence	1431:1438	arg1	GMMA					1473:1476	S. sonnei GMMA	1463:1476	S. sonnei GMMA	1463:1476	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	2	46	theme	based	331:335	arg1	vaccine					337:343	an O-Antigen (OAg) based vaccine	312:343	an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis	312:425	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	0	47	theme	proteins	45:52	arg1	contribution					15:26	the contribution	11:26	the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA)	11:142	Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).					
33441861	6	48	theme	main	961:964	arg1	drivers					966:972	the main drivers	957:972	the main drivers of bactericidal activity against OAg-positive bacteria	957:1027	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	6	48	theme	main	961:964	arg1	antibodies					941:950	anti-OAg antibodies	932:950	anti-OAg antibodies	932:950	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	3	49	theme	animal	566:571	arg1	models					573:578	animal models	566:578	animal models	566:578	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	11	50	theme	GMMA	1508:1511	arg1	vaccines					1513:1520	GMMA vaccines	1508:1520	GMMA vaccines	1508:1520	Hence, the presence of protein antigens on S. sonnei GMMA represents an added value for GMMA vaccines compared to other OAg-based formulations.					
33441861	6	51	theme	OAg	852:854	arg1	chains					856:861	OAg chains	852:861	OAg chains	852:861	Here we demonstrated that OAg chains shield the bacteria from anti-protein antibody binding and therefore anti-OAg antibodies were the main drivers of bactericidal activity against OAg-positive bacteria.					
33441861	5	52	with	immunization	765:776	arg1	GMMA					820:823	either OAg-negative and OAg-positive GMMA	783:823	either OAg-negative and OAg-positive GMMA	783:823	Anti-protein antibodies are induced in mice upon immunization with either OAg-negative and OAg-positive GMMA.					
33441861	10	53	theme	protein	1402:1408	arg1	exposure					1410:1417	protein exposure	1402:1417	protein exposure	1402:1417	However, little is known about OAg length and density regulation during infection and, therefore, protein exposure.					
33441861	3	54	theme	key	467:469	arg1	antigen					471:477	a key antigen	465:477	a key antigen for protective immunity	465:501	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	3	54	theme	key	467:469	arg1	OAg					438:440	S. sonnei OAg	428:440	S. sonnei OAg	428:440	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	4	55	theme	protein-specific	620:635	arg1	antibodies					637:646	protein-specific antibodies	620:646	protein-specific antibodies induced upon vaccination with GMMA	620:681	The contribution of protein-specific antibodies induced upon vaccination with GMMA has never been fully elucidated.					
33441861	3	56	theme	healthy	584:590	arg1	adults					592:597	healthy adults	584:597	healthy adults	584:597	S. sonnei OAg has been identified as a key antigen for protective immunity, and GMMA are able to induce anti-OAg-specific IgG response in animal models and healthy adults.					
33441861	1	57	theme	Gram-negative	188:200	arg1	bacteria					202:209	engineered Gram-negative bacteria	177:209	engineered Gram-negative bacteria resembling the composition of outer membranes	177:255	GMMA are exosomes released from engineered Gram-negative bacteria resembling the composition of outer membranes.					
33441861	1	58	theme	outer	241:245	arg1	membranes					247:255	outer membranes	241:255	outer membranes	241:255	GMMA are exosomes released from engineered Gram-negative bacteria resembling the composition of outer membranes.					
33441861	8	59	theme	proteomic	1188:1196	arg1	analysis					1198:1205	proteomic analysis	1188:1205	proteomic analysis	1188:1205	The immunodominant protein antigens were identified by proteomic analysis.					
33441861	5	60	theme	OAg-negative	790:801	arg1	GMMA					820:823	either OAg-negative and OAg-positive GMMA	783:823	either OAg-negative and OAg-positive GMMA	783:823	Anti-protein antibodies are induced in mice upon immunization with either OAg-negative and OAg-positive GMMA.					
33441861	2	61	theme	Shigella	353:360	arg1	cause					406:410	the most epidemiologically relevant cause	370:410	the most epidemiologically relevant cause of shigellosis	370:425	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	2	61	theme	Shigella	353:360	arg1	sonnei					362:367	Shigella sonnei	353:367	Shigella sonnei	353:367	We applied the GMMA technology for the development of an O-Antigen (OAg) based vaccine against Shigella sonnei, the most epidemiologically relevant cause of shigellosis.					
33441861	8	62	theme	immunodominant	1137:1150	arg1	antigens					1160:1167	The immunodominant protein antigens	1133:1167	The immunodominant protein antigens	1133:1167	The immunodominant protein antigens were identified by proteomic analysis.					
33441861	1	63	theme	membranes	247:255	arg1	composition					226:236	the composition	222:236	the composition of outer membranes	222:255	GMMA are exosomes released from engineered Gram-negative bacteria resembling the composition of outer membranes.					
34070644	0	0	theme	Media	73:77	arg1	Composition					79:89	Media Composition	73:89	Media Composition	73:89	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	0	1	from	Selectivity	15:25	arg1	solani					59:64	solani	59:64	solani	59:64	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	5	2	theme	interest	763:770	arg1	compounds					750:758	all the compounds	742:758	all the compounds of interest	742:770	Each combination displayed different selectivity and production yields for all the compounds of interest.					
34070644	2	3	theme	different	342:350	arg1	polyketides					352:362	four different polyketides	337:362	four different polyketides produced by Fusarium solani	337:390	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	6	4	theme	possible	825:832	arg1	yields					842:847	possible maximum yields	825:847	possible maximum yields for the surrounding combinations of media	825:889	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	3	5	theme	nitrogen	509:516	arg1	sources					518:524	nitrogen sources	509:524	nitrogen sources	509:524	We introduce four different carbohydrates and two types of nitrogen sources.					
34070644	0	6	from	Production	0:9	arg1	solani					59:64	solani	59:64	solani	59:64	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	7	7	theme	high	972:975	arg1	selectivity					977:987	high selectivity	972:987	high selectivity	972:987	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	7	7	theme	high	972:975	arg1	factor					1012:1017	an important factor	999:1017	an important factor for making purification steps easier	999:1054	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	2	8	theme	sources	278:284	arg1	influence					238:246	the influence	234:246	the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin	234:447	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	9	theme	nitrogen	269:276	arg1	sources					278:284	nitrogen sources	269:284	nitrogen sources	269:284	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	1	10	theme	structural	125:134	arg1	variation					136:144	a large structural variation	117:144	a large structural variation	117:144	Natural products display a large structural variation and different uses within a broad spectrum of industries.					
34070644	3	11	theme	different	468:476	arg1	carbohydrates					478:490	four different carbohydrates	463:490	four different carbohydrates	463:490	We introduce four different carbohydrates and two types of nitrogen sources.					
34070644	4	12	theme	nitrogen	657:664	arg1	types					648:652	the two types	640:652	the two types of nitrogen	640:664	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	4	13	theme	types	648:652	arg1	levels					630:635	three levels	624:635	three levels of the two types of nitrogen	624:664	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	4	13	theme	types	648:652	arg1	levels					602:607	three levels	596:607	three levels of sucrose	596:618	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	6	14	theme	surrounding	857:867	arg1	combinations					869:880	the surrounding combinations	853:880	the surrounding combinations of media	853:889	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	2	15	theme	carbohydrates	251:263	arg1	influence					238:246	the influence	234:246	the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin	234:447	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	1	16	dep	different	150:158	arg1	uses					160:163	uses	160:163	uses	160:163	Natural products display a large structural variation and different uses within a broad spectrum of industries.					
34070644	1	17	theme	broad	174:178	arg1	spectrum					180:187	a broad spectrum	172:187	a broad spectrum of industries	172:201	Natural products display a large structural variation and different uses within a broad spectrum of industries.					
34070644	6	18	theme	surface	782:788	arg1	design					790:795	Response surface design	773:795	Response surface design	773:795	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	8	19	theme	high	1137:1140	arg1	yields					1142:1147	high yields	1137:1147	high yields	1137:1147	We visualized the production over time for one of the media types, illustrating high yields and selectivity.					
34070644	4	20	theme	factorial	545:553	arg1	design					555:560	a full factorial design	538:560	a full factorial design	538:560	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	5	21	theme	different	694:702	arg1	selectivity					704:714	different selectivity	694:714	different selectivity	694:714	Each combination displayed different selectivity and production yields for all the compounds of interest.					
34070644	6	22	theme	Response	773:780	arg1	design					790:795	Response surface design	773:795	Response surface design	773:795	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	1	23	theme	Natural	92:98	arg1	products					100:107	Natural products	92:107	Natural products	92:107	Natural products display a large structural variation and different uses within a broad spectrum of industries.					
34070644	4	24	theme	levels	630:635	arg1	combinations					580:591	combinations	580:591	combinations of three levels of sucrose and three levels of the two types of nitrogen	580:664	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	6	25	theme	media	885:889	arg1	combinations					869:880	the surrounding combinations	853:880	the surrounding combinations of media	853:889	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	1	26	theme	industries	192:201	arg1	spectrum					180:187	a broad spectrum	172:187	a broad spectrum of industries	172:201	Natural products display a large structural variation and different uses within a broad spectrum of industries.					
34070644	0	27	theme	Fusarubins	34:43	arg1	Selectivity					15:25	Selectivity	15:25	Selectivity	15:25	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	0	27	theme	Fusarubins	34:43	arg1	Production					0:9	Production	0:9	Production	0:9	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	2	28	from	influence	238:246	arg1	bostrycoidin					415:426	bostrycoidin	415:426	bostrycoidin	415:426	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	28	from	influence	238:246	arg1	production					293:302	production	293:302	production	293:302	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	28	from	influence	238:246	arg1	anhydrofusarubin					432:447	anhydrofusarubin	432:447	anhydrofusarubin	432:447	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	28	from	influence	238:246	arg1	fusarubin					393:401	fusarubin	393:401	fusarubin	393:401	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	28	from	influence	238:246	arg1	selectivity					308:318	selectivity	308:318	selectivity	308:318	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	28	from	influence	238:246	arg1	javanicin					404:412	javanicin	404:412	javanicin	404:412	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	0	29	theme	Key	30:32	arg1	Fusarubins					34:43	Key Fusarubins	30:43	Key Fusarubins from Fusarium solani	30:64	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	5	30	theme	production	720:729	arg1	yields					731:736	production yields	720:736	production yields	720:736	Each combination displayed different selectivity and production yields for all the compounds of interest.					
34070644	0	31	from	solani	59:64	arg1	Selectivity					15:25	Selectivity	15:25	Selectivity	15:25	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	0	31	from	solani	59:64	arg1	Fusarubins					34:43	Key Fusarubins	30:43	Key Fusarubins from Fusarium solani	30:64	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	0	31	from	solani	59:64	arg1	Production					0:9	Production	0:9	Production	0:9	Production and Selectivity of Key Fusarubins from Fusarium solani due to Media Composition.					
34070644	4	32	theme	full	540:543	arg1	design					555:560	a full factorial design	538:560	a full factorial design	538:560	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	2	33	theme	production	323:332	arg1	bostrycoidin					415:426	bostrycoidin	415:426	bostrycoidin	415:426	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	33	theme	production	323:332	arg1	production					293:302	production	293:302	production	293:302	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	33	theme	production	323:332	arg1	anhydrofusarubin					432:447	anhydrofusarubin	432:447	anhydrofusarubin	432:447	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	33	theme	production	323:332	arg1	fusarubin					393:401	fusarubin	393:401	fusarubin	393:401	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	33	theme	production	323:332	arg1	selectivity					308:318	selectivity	308:318	selectivity	308:318	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	2	33	theme	production	323:332	arg1	javanicin					404:412	javanicin	404:412	javanicin	404:412	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	8	34	theme	media	1111:1115	arg1	types					1117:1121	the media types	1107:1121	the media types	1107:1121	We visualized the production over time for one of the media types, illustrating high yields and selectivity.					
34070644	7	35	theme	important	1002:1010	arg1	selectivity					977:987	high selectivity	972:987	high selectivity	972:987	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	7	35	theme	important	1002:1010	arg1	factor					1012:1017	an important factor	999:1017	an important factor for making purification steps easier	999:1054	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	1	36	theme	large	119:123	arg1	variation					136:144	a large structural variation	117:144	a large structural variation	117:144	Natural products display a large structural variation and different uses within a broad spectrum of industries.					
34070644	7	37	theme	maximum	919:925	arg1	ones					954:957	the ones	950:957	the ones illustrating high selectivity, which is an important factor for making purification steps easier	950:1054	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	7	37	theme	maximum	919:925	arg1	yields					927:932	the maximum yields	915:932	the maximum yields	915:932	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	7	38	theme	purification	1030:1041	arg1	steps					1043:1047	purification steps	1030:1047	purification steps	1030:1047	It was also shown that the maximum yields were not always the ones illustrating high selectivity, which is an important factor for making purification steps easier.					
34070644	2	39	dep	production	293:302	arg1	the					289:291	the	289:291	the	289:291	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	4	40	theme	levels	602:607	arg1	combinations					580:591	combinations	580:591	combinations of three levels of sucrose and three levels of the two types of nitrogen	580:664	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	6	41	theme	maximum	834:840	arg1	yields					842:847	possible maximum yields	825:847	possible maximum yields for the surrounding combinations of media	825:889	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	6	42	used	utilized	801:808	arg2	design					790:795	Response surface design	773:795	Response surface design	773:795	Response surface design was utilized to investigate possible maximum yields for the surrounding combinations of media.					
34070644	3	43	theme	sources	518:524	arg1	carbohydrates					478:490	four different carbohydrates	463:490	four different carbohydrates	463:490	We introduce four different carbohydrates and two types of nitrogen sources.					
34070644	3	43	theme	sources	518:524	arg1	types					500:504	two types	496:504	two types of nitrogen sources	496:524	We introduce four different carbohydrates and two types of nitrogen sources.					
34070644	8	44	theme	types	1117:1121	arg1	types					1117:1121	the media types	1107:1121	the media types	1107:1121	We visualized the production over time for one of the media types, illustrating high yields and selectivity.					
34070644	8	44	theme	types	1117:1121	arg1	one					1100:1102	one	1100:1102	one	1100:1102	We visualized the production over time for one of the media types, illustrating high yields and selectivity.					
34070644	2	45	theme	polyketides	352:362	arg1	production					323:332	production	323:332	production of four different polyketides produced by Fusarium solani	323:390	In this study, we investigate the influence of carbohydrates and nitrogen sources on the production and selectivity of production of four different polyketides produced by Fusarium solani, fusarubin, javanicin, bostrycoidin and anhydrofusarubin.					
34070644	4	46	theme	sucrose	612:618	arg1	levels					630:635	three levels	624:635	three levels of the two types of nitrogen	624:664	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
34070644	4	46	theme	sucrose	612:618	arg1	levels					602:607	three levels	596:607	three levels of sucrose	596:618	Hereafter, a full factorial design was applied using combinations of three levels of sucrose and three levels of the two types of nitrogen.					
32777426	7	0	used	used	1175:1178	arg2	xylan					1165:1169	birchwood xylan	1155:1169	birchwood xylan	1155:1169	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	7	0	used	used	1175:1178	arg2	substrate					1187:1195	the substrate	1183:1195	the substrate	1183:1195	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	6	1	theme	highest	952:958	arg1	level					960:964	the highest level	948:964	the highest level of xylose	948:974	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	4	2	theme	T-Xyn	731:735	arg1	temperature					694:704	optimum temperature	686:704	optimum temperature	686:704	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	4	2	theme	T-Xyn	731:735	arg1	stability					718:726	thermal stability	710:726	thermal stability	710:726	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	5	3	contain	has	772:774	arg2	influence					785:793	a strong influence	776:793	a strong influence	776:793	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	5	3	contain	has	772:774	arg1	existence					742:750	The existence	738:750	The existence of a disulfide bond	738:770	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	1	4	theme	hydrolytic	231:240	arg1	activity					242:249	hydrolytic activity	231:249	hydrolytic activity	231:249	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	0	5	theme	xylanase	64:71	arg1	characteristics					43:57	hydrolytic characteristics	32:57	hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208	32:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	2	6	from	present	324:330	arg1	T-Xyn					345:349	wild-type T-Xyn	335:349	wild-type T-Xyn	335:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	6	7	dep	produces	939:946	arg1	whereas					1021:1027	whereas	1021:1027	whereas	1021:1027	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	5	8	dep	composition	867:877	arg1	the					863:865	the	863:865	the	863:865	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	0	9	from	Impact	0:5	arg1	characteristics					43:57	hydrolytic characteristics	32:57	hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208	32:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	7	10	theme	birchwood	1155:1163	arg1	substrate					1187:1195	the substrate	1183:1195	the substrate	1183:1195	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	7	10	theme	birchwood	1155:1163	arg1	xylan					1165:1169	birchwood xylan	1155:1169	birchwood xylan	1155:1169	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	3	11	theme	T-Xyn-T38C-S50C	524:538	arg1	lower					576:580	lower	576:580	lower	576:580	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S was lower than that of T-Xyn and T-Xyn-C122S-C166S.					
32777426	3	11	theme	T-Xyn-T38C-S50C	524:538	arg1	pH					518:519	The optimum pH	506:519	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S	506:570	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S was lower than that of T-Xyn and T-Xyn-C122S-C166S.					
32777426	5	12	theme	products	913:920	arg1	proportion					883:892	proportion	883:892	proportion	883:892	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	5	12	theme	products	913:920	arg1	composition					867:877	composition	867:877	composition	867:877	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	0	13	theme	Talaromyces	78:88	arg1	thermophiles					90:101	Talaromyces thermophiles F1208	78:107	Talaromyces thermophiles F1208	78:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	0	14	from	characteristics	43:57	arg1	thermophiles					90:101	Talaromyces thermophiles F1208	78:107	Talaromyces thermophiles F1208	78:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	1	15	from	thermophiles	138:149	arg1	xylanase					112:119	A xylanase	110:119	A xylanase from Talaromyces thermophiles F1208 (T-Xyn)	110:163	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	6	16	theme	xylotriose	1138:1147	arg1	xylotriose					1138:1147	xylotriose	1138:1147	xylotriose	1138:1147	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	6	16	theme	xylotriose	1138:1147	arg1	amount					1128:1133	the largest amount	1116:1133	the largest amount of xylotriose	1116:1147	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	5	17	theme	bond	767:770	arg1	existence					742:750	The existence	738:750	The existence of a disulfide bond	738:770	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	3	18	theme	T-Xyn-T38C-S50C-C122S-C166S	544:570	arg1	lower					576:580	lower	576:580	lower	576:580	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S was lower than that of T-Xyn and T-Xyn-C122S-C166S.					
32777426	3	18	theme	T-Xyn-T38C-S50C-C122S-C166S	544:570	arg1	pH					518:519	The optimum pH	506:519	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S	506:570	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S was lower than that of T-Xyn and T-Xyn-C122S-C166S.					
32777426	4	19	theme	optimum	686:692	arg1	temperature					694:704	optimum temperature	686:704	optimum temperature	686:704	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	7	20	theme	bond	1230:1233	arg1	introduction					1202:1213	the introduction	1198:1213	the introduction of a disulfide bond	1198:1233	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	2	21	theme	T-Xyn-C122S-C166S	256:272	arg1	mutant					274:279	The T-Xyn-C122S-C166S mutant	252:279	The T-Xyn-C122S-C166S mutant	252:279	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	6	22	theme	largest	1120:1126	arg1	xylotriose					1138:1147	xylotriose	1138:1147	xylotriose	1138:1147	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	6	22	theme	largest	1120:1126	arg1	amount					1128:1133	the largest amount	1116:1133	the largest amount of xylotriose	1116:1147	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	5	23	theme	T-Xyn	832:836	arg1	characteristics					813:827	the hydrolysis characteristics	798:827	the hydrolysis characteristics of T-Xyn	798:836	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	2	24	attach	present	324:330	arg2	bond					319:322	the C122-C166 disulfide bond	295:322	the C122-C166 disulfide bond present in wild-type T-Xyn	295:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	24	attach	present	324:330	arg1	T-Xyn					345:349	wild-type T-Xyn	335:349	wild-type T-Xyn	335:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	25	from	T-Xyn	345:349	arg1	present					324:330	present	324:330	present	324:330	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	26	theme	disulfide	309:317	arg1	bond					319:322	the C122-C166 disulfide bond	295:322	the C122-C166 disulfide bond present in wild-type T-Xyn	295:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	27	theme	T-Xyn-T38C-S50C-C122S-C166S	380:406	arg1	mutants					408:414	T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants	360:414	T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants	360:414	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	8	28	with	complex	1405:1411	arg1	introduction					1422:1433	the introduction	1418:1433	the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose	1418:1513	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	2	29	theme	C122-C166	299:307	arg1	bond					319:322	the C122-C166 disulfide bond	295:322	the C122-C166 disulfide bond present in wild-type T-Xyn	295:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	0	30	theme	disulfide	14:22	arg1	bond					24:27	the disulfide bond	10:27	the disulfide bond	10:27	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	1	31	dep	thermophiles	138:149	arg1	F1208					151:155	F1208	151:155	Talaromyces thermophiles F1208 (T-Xyn)	126:163	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	1	31	dep	thermophiles	138:149	arg1	T-Xyn					158:162	T-Xyn	158:162	T-Xyn	158:162	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	4	32	from	decrease	670:677	arg1	temperature					694:704	optimum temperature	686:704	optimum temperature	686:704	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	4	32	from	decrease	670:677	arg1	stability					718:726	thermal stability	710:726	thermal stability	710:726	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	7	33	theme	polymerization	1325:1338	arg1	degree					1315:1320	a high degree	1308:1320	a high degree of polymerization (DP ≥ 5)	1308:1347	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	8	34	theme	oat-spelt	1381:1389	arg1	xylan					1391:1395	oat-spelt xylan	1381:1395	oat-spelt xylan	1381:1395	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	2	35	theme	disulfide	435:443	arg1	C38-C50					451:457	C38-C50	451:457	C38-C50	451:457	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	35	theme	disulfide	435:443	arg1	bond					445:448	an introduced disulfide bond	421:448	an introduced disulfide bond	421:448	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	3	36	theme	optimum	510:516	arg1	lower					576:580	lower	576:580	lower	576:580	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S was lower than that of T-Xyn and T-Xyn-C122S-C166S.					
32777426	3	36	theme	optimum	510:516	arg1	pH					518:519	The optimum pH	506:519	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S	506:570	The optimum pH of T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S was lower than that of T-Xyn and T-Xyn-C122S-C166S.					
32777426	6	37	theme	xylobiose	1065:1073	arg1	level					1056:1060	the highest level	1044:1060	the highest level of xylobiose	1044:1073	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	6	38	theme	beechwood	987:995	arg1	xylan					997:1001	beechwood xylan	987:1001	beechwood xylan	987:1001	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	6	38	theme	beechwood	987:995	arg1	substrate					1010:1018	the substrate	1006:1018	the substrate	1006:1018	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	2	39	theme	introduced	424:433	arg1	C38-C50					451:457	C38-C50	451:457	C38-C50	451:457	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	39	theme	introduced	424:433	arg1	bond					445:448	an introduced disulfide bond	421:448	an introduced disulfide bond	421:448	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	0	40	theme	bond	24:27	arg1	Impact					0:5	Impact	0:5	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.	0:108	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	2	41	contain	have	290:293	arg2	bond					319:322	the C122-C166 disulfide bond	295:322	the C122-C166 disulfide bond present in wild-type T-Xyn	295:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	41	contain	have	290:293	arg1	mutant					274:279	The T-Xyn-C122S-C166S mutant	252:279	The T-Xyn-C122S-C166S mutant	252:279	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	5	42	theme	hydrolysis	802:811	arg1	characteristics					813:827	the hydrolysis characteristics	798:827	the hydrolysis characteristics of T-Xyn	798:836	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	5	43	theme	disulfide	757:765	arg1	bond					767:770	a disulfide bond	755:770	a disulfide bond	755:770	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	4	44	theme	bond	656:659	arg1	introduction					628:639	The introduction	624:639	The introduction of a disulfide bond	624:659	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	8	45	from	increase	1468:1475	arg1	rate					1496:1499	the degradation rate	1480:1499	the degradation rate of xylotriose	1480:1513	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	4	46	dep	temperature	694:704	arg1	the					682:684	the	682:684	the	682:684	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	8	47	theme	degradation	1484:1494	arg1	rate					1496:1499	the degradation rate	1480:1499	the degradation rate of xylotriose	1480:1513	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	5	48	theme	hydrolysate	901:911	arg1	products					913:920	the hydrolysate products	897:920	the hydrolysate products	897:920	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	8	49	theme	xylan	1391:1395	arg1	hydrolysis					1367:1376	The hydrolysis	1363:1376	The hydrolysis of oat-spelt xylan	1363:1395	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	8	49	theme	xylan	1391:1395	arg1	complex					1405:1411	complex	1405:1411	complex	1405:1411	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	2	50	contain	have	416:419	arg1	mutants					408:414	T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants	360:414	T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants	360:414	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	50	contain	have	416:419	arg2	bond					445:448	an introduced disulfide bond	421:448	an introduced disulfide bond	421:448	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	2	50	contain	have	416:419	arg2	C38-C50					451:457	C38-C50	451:457	C38-C50	451:457	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	8	51	theme	xylotriose	1504:1513	arg1	rate					1496:1499	the degradation rate	1480:1499	the degradation rate of xylotriose	1480:1513	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	1	52	from	effects	202:208	arg1	activity					242:249	hydrolytic activity	231:249	hydrolytic activity	231:249	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	2	53	theme	wild-type	335:343	arg1	T-Xyn					345:349	wild-type T-Xyn	335:349	wild-type T-Xyn	335:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	0	54	theme	hydrolytic	32:41	arg1	characteristics					43:57	hydrolytic characteristics	32:57	hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208	32:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	6	55	theme	xylose	969:974	arg1	level					960:964	the highest level	948:964	the highest level of xylose	948:974	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	5	56	from	influence	785:793	arg1	characteristics					813:827	the hydrolysis characteristics	798:827	the hydrolysis characteristics of T-Xyn	798:836	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	5	57	from	changes	852:858	arg1	proportion					883:892	proportion	883:892	proportion	883:892	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	5	57	from	changes	852:858	arg1	composition					867:877	composition	867:877	composition	867:877	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	2	58	dep	have	290:293	arg1	whereas					352:358	whereas	352:358	whereas	352:358	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	8	59	theme	disulfide	1442:1450	arg1	bond					1452:1455	the disulfide bond	1438:1455	the disulfide bond causing an increase in the degradation rate of xylotriose	1438:1513	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	7	60	dep	increased	1235:1243	arg1	decreased					1268:1276	decreased	1268:1276	decreased the content of xylotriose	1268:1302	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	6	61	theme	highest	1048:1054	arg1	level					1056:1060	the highest level	1044:1060	the highest level of xylobiose	1044:1073	T-Xyn-T38C-S50C produces the highest level of xylose when using beechwood xylan as the substrate, whereas T-Xyn produces the highest level of xylobiose and T-Xyn-T38C-S50C-C122S-C166S produces the largest amount of xylotriose.					
32777426	2	62	theme	present	324:330	arg1	bond					319:322	the C122-C166 disulfide bond	295:322	the C122-C166 disulfide bond present in wild-type T-Xyn	295:349	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	0	63	from	thermophiles	90:101	arg1	xylanase					64:71	a xylanase	62:71	a xylanase from Talaromyces thermophiles F1208	62:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	0	63	from	thermophiles	90:101	arg1	characteristics					43:57	hydrolytic characteristics	32:57	hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208	32:107	Impact of the disulfide bond on hydrolytic characteristics of a xylanase from Talaromyces thermophiles F1208.					
32777426	1	64	used	used	169:172	arg2	xylanase					112:119	A xylanase	110:119	A xylanase from Talaromyces thermophiles F1208 (T-Xyn)	110:163	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	1	65	theme	Talaromyces	126:136	arg1	thermophiles					138:149	Talaromyces thermophiles F1208 (T-Xyn)	126:163	Talaromyces thermophiles F1208 (T-Xyn)	126:163	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	1	66	theme	disulfide	213:221	arg1	bond					223:226	disulfide bond	213:226	disulfide bond	213:226	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	7	67	theme	xylose	1260:1265	arg1	content					1249:1255	the content	1245:1255	the content of xylose	1245:1265	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	8	68	theme	bond	1452:1455	arg1	introduction					1422:1433	the introduction	1418:1433	the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose	1418:1513	The hydrolysis of oat-spelt xylan is more complex with the introduction of the disulfide bond causing an increase in the degradation rate of xylotriose.					
32777426	2	69	theme	T-Xyn-T38C-S50C	360:374	arg1	mutants					408:414	T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants	360:414	T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants	360:414	The T-Xyn-C122S-C166S mutant does not have the C122-C166 disulfide bond present in wild-type T-Xyn, whereas T-Xyn-T38C-S50C and T-Xyn-T38C-S50C-C122S-C166S mutants have an introduced disulfide bond, C38-C50, to T-Xyn and T-Xyn-C122S-C166S, respectively.					
32777426	1	70	theme	bond	223:226	arg1	effects					202:208	the effects	198:208	the effects of disulfide bond on hydrolytic activity	198:249	A xylanase from Talaromyces thermophiles F1208 (T-Xyn) was used specifically to explore the effects of disulfide bond on hydrolytic activity.					
32777426	5	71	theme	strong	778:783	arg1	influence					785:793	a strong influence	776:793	a strong influence	776:793	The existence of a disulfide bond has a strong influence on the hydrolysis characteristics of T-Xyn, which caused changes in the composition and proportion of the hydrolysate products.					
32777426	7	72	theme	disulfide	1220:1228	arg1	bond					1230:1233	a disulfide bond	1218:1233	a disulfide bond	1218:1233	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	7	73	theme	high	1310:1313	arg1	degree					1315:1320	a high degree	1308:1320	a high degree of polymerization (DP ≥ 5)	1308:1347	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	7	74	theme	xylotriose	1293:1302	arg1	content					1282:1288	the content	1278:1288	the content of xylotriose	1278:1302	When birchwood xylan was used as the substrate, the introduction of a disulfide bond increased the content of xylose, decreased the content of xylotriose and a high degree of polymerization (DP ≥ 5) was observed.					
32777426	4	75	theme	thermal	710:716	arg1	stability					718:726	thermal stability	710:726	thermal stability	710:726	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32777426	4	76	theme	disulfide	646:654	arg1	bond					656:659	a disulfide bond	644:659	a disulfide bond	644:659	The introduction of a disulfide bond caused a decrease in the optimum temperature and thermal stability of T-Xyn.					
32207757	0	0	theme	gelation	107:114	arg1	microfibers					137:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	5	1	theme	magnetic	782:789	arg1	template					791:798	a magnetic template	780:798	a magnetic template	780:798	A 9 × 9 micropillar array surrounded by a magnetic substrate was constructed to form 8 × 8 microgaps arranged in a crisscross pattern as a magnetic template.					
32207757	7	2	theme	various	1165:1171	arg1	patterns					1191:1198	various organized spatial patterns	1165:1198	various organized spatial patterns	1165:1198	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	9	3	with	constructs	1475:1484	arg1	composition					1516:1526	organized spatiotemporal composition	1491:1526	organized spatiotemporal composition	1491:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	1	4	theme	methacrylate	180:191	arg1	microfibers					201:211	gelatin methacrylate (GelMA) microfibers	172:211	gelatin methacrylate (GelMA) microfibers	172:211	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
32207757	6	5	theme	assembly	930:937	arg1	accuracy					939:946	micron assembly accuracy	923:946	micron assembly accuracy	923:946	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	4	6	theme	spinning	626:633	arg1	method					635:640	a microfluidic spinning method	611:640	a microfluidic spinning method	611:640	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	0	7	theme	low-concentration	89:105	arg1	microfibers					137:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	5	8	theme	magnetic	685:692	arg1	substrate					694:702	a magnetic substrate	683:702	a magnetic substrate	683:702	A 9 × 9 micropillar array surrounded by a magnetic substrate was constructed to form 8 × 8 microgaps arranged in a crisscross pattern as a magnetic template.					
32207757	8	9	theme	low	1214:1216	arg1	requirements					1238:1249	the low mechanical property requirements	1210:1249	the low mechanical property requirements of assembled microfibers and the easy-to-implement operation	1210:1310	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	8	10	theme	microfluidic	1390:1401	arg1	microfibers					1408:1418	various microfluidic spun microfibers	1382:1418	various microfluidic spun microfibers	1382:1418	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	8	11	theme	easy-to-implement	1284:1300	arg1	operation					1302:1310	the easy-to-implement operation	1280:1310	the easy-to-implement operation	1280:1310	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	4	12	theme	microfluidic	613:624	arg1	method					635:640	a microfluidic spinning method	611:640	a microfluidic spinning method	611:640	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	6	13	theme	magnetic	819:826	arg1	attraction					828:837	magnetic attraction	819:837	magnetic attraction	819:837	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	0	14	theme	methacrylate	116:127	arg1	microfibers					137:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	4	15	theme	GelMA	529:533	arg1	microfibers					535:545	GelMA microfibers	529:545	GelMA microfibers (5%, w/v) containing magnetic nanoparticles	529:589	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	8	16	theme	microfibers	1408:1418	arg1	assembly					1370:1377	the assembly	1366:1377	the assembly of various microfluidic spun microfibers	1366:1418	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	5	17	theme	×	647:647	arg1	array					663:667	A 9 × 9 micropillar array	643:667	A 9 × 9 micropillar array surrounded by a magnetic substrate	643:702	A 9 × 9 micropillar array surrounded by a magnetic substrate was constructed to form 8 × 8 microgaps arranged in a crisscross pattern as a magnetic template.					
32207757	2	18	theme	GelMA	325:329	arg1	microfibers					331:341	low-concentration GelMA microfibers	307:341	low-concentration GelMA microfibers	307:341	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	2	18	theme	GelMA	325:329	arg1	blocks					355:360	building blocks	346:360	building blocks for higher-order cellular assembly	346:395	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	5	19	theme	crisscross	758:767	arg1	pattern					769:775	a crisscross pattern	756:775	a crisscross pattern as a magnetic template	756:798	A 9 × 9 micropillar array surrounded by a magnetic substrate was constructed to form 8 × 8 microgaps arranged in a crisscross pattern as a magnetic template.					
32207757	6	20	theme	micron	923:928	arg1	accuracy					939:946	micron assembly accuracy	923:946	micron assembly accuracy	923:946	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	5	21	theme	micropillar	651:661	arg1	array					663:667	A 9 × 9 micropillar array	643:667	A 9 × 9 micropillar array surrounded by a magnetic substrate	643:702	A 9 × 9 micropillar array surrounded by a magnetic substrate was constructed to form 8 × 8 microgaps arranged in a crisscross pattern as a magnetic template.					
32207757	2	22	theme	low-concentration	307:323	arg1	microfibers					331:341	low-concentration GelMA microfibers	307:341	low-concentration GelMA microfibers	307:341	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	2	22	theme	low-concentration	307:323	arg1	blocks					355:360	building blocks	346:360	building blocks for higher-order cellular assembly	346:395	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	1	23	theme	microfibers	201:211	arg1	concentrations					154:167	Low concentrations	150:167	Low concentrations of gelatin methacrylate (GelMA) microfibers	150:211	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
32207757	3	24	theme	template-based	484:497	arg1	method					499:504	a new template-based method	478:504	a new template-based method to solve this problem	478:526	Herein, we report a new template-based method to solve this problem.					
32207757	0	25	theme	GelMA	130:134	arg1	microfibers					137:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	6	26	with	template	909:916	arg1	accuracy					939:946	micron assembly accuracy	923:946	micron assembly accuracy	923:946	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	4	27	contain	containing	557:566	arg1	microfibers					535:545	GelMA microfibers	529:545	GelMA microfibers (5%, w/v) containing magnetic nanoparticles	529:589	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	4	27	contain	containing	557:566	arg2	nanoparticles					577:589	magnetic nanoparticles	568:589	magnetic nanoparticles	568:589	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	8	28	theme	operation	1302:1310	arg1	requirements					1238:1249	the low mechanical property requirements	1210:1249	the low mechanical property requirements of assembled microfibers and the easy-to-implement operation	1210:1310	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	7	29	theme	cells	1126:1130	arg1	activities					1081:1090	the activities	1077:1090	the activities of surface seeded or encapsulated cells	1077:1130	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	6	30	theme	DMEM	804:807	arg1	solution					809:816	DMEM solution	804:816	DMEM solution	804:816	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	2	31	theme	mechanical	437:446	arg1	properties					448:457	their poor mechanical properties	426:457	their poor mechanical properties	426:457	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	0	32	theme	Template-based	0:13	arg1	fabrication					15:25	Template-based fabrication	0:25	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.	0:148	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	6	33	theme	microgrid-like	956:969	arg1	microGC					982:988	microGC	982:988	microGC	982:988	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	6	33	theme	microgrid-like	956:969	arg1	construct					971:979	a microgrid-like construct	954:979	a microgrid-like construct (microGC) generated after removing all micropillars	954:1031	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	2	34	theme	poor	432:435	arg1	properties					448:457	their poor mechanical properties	426:457	their poor mechanical properties	426:457	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	8	35	theme	assembled	1254:1262	arg1	microfibers					1264:1274	assembled microfibers	1254:1274	assembled microfibers	1254:1274	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	4	36	dep	microfibers	535:545	arg1	%					549:549	5%	548:549	5%	548:549	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	4	36	dep	microfibers	535:545	arg1	w/v					552:554	w/v	552:554	w/v	552:554	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	9	37	theme	convenient	1536:1545	arg1	platform					1547:1554	a convenient platform	1534:1554	a convenient platform for the study of tissue engineering	1534:1590	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	2	38	theme	building	346:353	arg1	microfibers					331:341	low-concentration GelMA microfibers	307:341	low-concentration GelMA microfibers	307:341	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	2	38	theme	building	346:353	arg1	blocks					355:360	building blocks	346:360	building blocks for higher-order cellular assembly	346:395	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	9	39	theme	microgrid-like	1451:1464	arg1	constructs					1475:1484	the resulting 3D microgrid-like cellular constructs	1434:1484	the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition	1434:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	7	40	theme	encapsulated	1113:1124	arg1	cells					1126:1130	surface seeded or encapsulated cells	1095:1130	surface seeded or encapsulated cells	1095:1130	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	0	41	theme	3D	50:51	arg1	constructs					63:72	spatially organized 3D bioactive constructs	30:72	spatially organized 3D bioactive constructs	30:72	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	9	42	theme	tissue	1573:1578	arg1	engineering					1580:1590	tissue engineering	1573:1590	tissue engineering	1573:1590	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	3	43	theme	new	480:482	arg1	method					499:504	a new template-based method	478:504	a new template-based method to solve this problem	478:526	Herein, we report a new template-based method to solve this problem.					
32207757	8	44	theme	versatile	1344:1352	arg1	pathway					1354:1360	a versatile pathway	1342:1360	a versatile pathway for the assembly of various microfluidic spun microfibers	1342:1418	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	0	45	theme	organized	40:48	arg1	constructs					63:72	spatially organized 3D bioactive constructs	30:72	spatially organized 3D bioactive constructs	30:72	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	7	46	theme	seeded	1103:1108	arg1	cells					1126:1130	surface seeded or encapsulated cells	1095:1130	surface seeded or encapsulated cells	1095:1130	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	9	47	theme	engineering	1580:1590	arg1	study					1564:1568	the study	1560:1568	the study of tissue engineering	1560:1590	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	9	48	theme	spatiotemporal	1501:1514	arg1	composition					1516:1526	organized spatiotemporal composition	1491:1526	organized spatiotemporal composition	1491:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	1	49	theme	cellular	236:243	arg1	activity					245:252	cellular activity	236:252	cellular activity	236:252	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
32207757	0	50	theme	constructs	63:72	arg1	fabrication					15:25	Template-based fabrication	0:25	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.	0:148	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	9	51	theme	resulting	1438:1446	arg1	constructs					1475:1484	the resulting 3D microgrid-like cellular constructs	1434:1484	the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition	1434:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	8	52	theme	microfibers	1264:1274	arg1	requirements					1238:1249	the low mechanical property requirements	1210:1249	the low mechanical property requirements of assembled microfibers and the easy-to-implement operation	1210:1310	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	6	53	theme	microfibers	880:890	arg1	arrangement					861:871	efficient arrangement	851:871	efficient arrangement of the microfibers according to the template with micron assembly accuracy	851:946	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	0	54	theme	bioactive	53:61	arg1	constructs					63:72	spatially organized 3D bioactive constructs	30:72	spatially organized 3D bioactive constructs	30:72	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	9	55	theme	3D	1448:1449	arg1	constructs					1475:1484	the resulting 3D microgrid-like cellular constructs	1434:1484	the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition	1434:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	7	56	theme	organized	1173:1181	arg1	patterns					1191:1198	various organized spatial patterns	1165:1198	various organized spatial patterns	1165:1198	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	4	57	theme	magnetic	568:575	arg1	nanoparticles					577:589	magnetic nanoparticles	568:589	magnetic nanoparticles	568:589	GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method.					
32207757	8	58	theme	proposed	1317:1324	arg1	method					1326:1331	the proposed method	1313:1331	the proposed method	1313:1331	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	1	59	theme	Low	150:152	arg1	concentrations					154:167	Low concentrations	150:167	Low concentrations of gelatin methacrylate (GelMA) microfibers	150:211	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
32207757	9	60	theme	cellular	1466:1473	arg1	constructs					1475:1484	the resulting 3D microgrid-like cellular constructs	1434:1484	the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition	1434:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	8	61	theme	property	1229:1236	arg1	requirements					1238:1249	the low mechanical property requirements	1210:1249	the low mechanical property requirements of assembled microfibers and the easy-to-implement operation	1210:1310	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	2	62	theme	higher-order	366:377	arg1	assembly					388:395	higher-order cellular assembly	366:395	higher-order cellular assembly	366:395	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	8	63	theme	spun	1403:1406	arg1	microfibers					1408:1418	various microfluidic spun microfibers	1382:1418	various microfluidic spun microfibers	1382:1418	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	2	64	theme	cellular	379:386	arg1	assembly					388:395	higher-order cellular assembly	366:395	higher-order cellular assembly	366:395	However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties.					
32207757	0	65	theme	magnetic	80:87	arg1	microfibers					137:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	magnetic low-concentration gelation methacrylate (GelMA) microfibers	80:147	Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.					
32207757	6	66	theme	efficient	851:859	arg1	arrangement					861:871	efficient arrangement	851:871	efficient arrangement of the microfibers according to the template with micron assembly accuracy	851:946	In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars.					
32207757	8	67	theme	mechanical	1218:1227	arg1	requirements					1238:1249	the low mechanical property requirements	1210:1249	the low mechanical property requirements of assembled microfibers and the easy-to-implement operation	1210:1310	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	8	68	theme	various	1382:1388	arg1	microfibers					1408:1418	various microfluidic spun microfibers	1382:1418	various microfluidic spun microfibers	1382:1418	Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers.					
32207757	7	69	dep	seeded	1103:1108	arg1	surface					1095:1101	surface	1095:1101	surface	1095:1101	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	7	70	theme	spatial	1183:1189	arg1	patterns					1191:1198	various organized spatial patterns	1165:1198	various organized spatial patterns	1165:1198	MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns.					
32207757	9	71	theme	organized	1491:1499	arg1	composition					1516:1526	organized spatiotemporal composition	1491:1526	organized spatiotemporal composition	1491:1526	Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.					
32207757	1	72	theme	gelatin	172:178	arg1	GelMA					194:198	GelMA	194:198	GelMA	194:198	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
32207757	1	72	theme	gelatin	172:178	arg1	methacrylate					180:191	gelatin methacrylate	172:191	gelatin methacrylate (GelMA) microfibers	172:211	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
32207757	1	73	theme	high	268:271	arg1	concentrations					273:286	high concentrations	268:286	high concentrations	268:286	Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations.					
33736295	0	0	theme	crosslinking	83:94	arg1	reaction					96:103	crosslinking reaction	83:103	crosslinking reaction	83:103	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	4	1	theme	chemical	668:675	arg1	structures					677:686	The physical and chemical structures	651:686	The physical and chemical structures of the composites	651:704	The physical and chemical structures of the composites were analyzed with SEM, STEM, XRD, FTIR, and TGA.					
33736295	1	2	theme	surface	251:257	arg1	area					259:262	high surface area	246:262	high surface area	246:262	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	5	3	theme	water	944:948	arg1	absorption					950:959	high water absorption	939:959	high water absorption (>1300%)	939:968	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	3	theme	water	944:948	arg1	%					967:967	>1300%	962:967	>1300%	962:967	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	1	4	theme	research	163:170	arg1	interests					172:180	research interests	163:180	research interests	163:180	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	6	5	theme	cells	1159:1163	arg1	proliferation					1127:1139	the proliferation	1123:1139	the proliferation of rat osteoblast cells	1123:1163	Moreover, our study further indicated that these CNCs/HAP materials could increase the proliferation of rat osteoblast cells.					
33736295	6	6	theme	osteoblast	1148:1157	arg1	cells					1159:1163	rat osteoblast cells	1144:1163	rat osteoblast cells	1144:1163	Moreover, our study further indicated that these CNCs/HAP materials could increase the proliferation of rat osteoblast cells.					
33736295	3	7	theme	in	527:528	arg1	mineralization					535:548	the in situ mineralization	523:548	the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface	523:602	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	5	8	dep	lightweight	885:895	arg1	foams					870:874	CNCs-based foams	859:874	CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa)	859:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	8	dep	lightweight	885:895	arg1	porous					920:925	porous	920:925	porous	920:925	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	8	dep	lightweight	885:895	arg1	50-75 mg/cm3					898:909	50-75 mg/cm3	898:909	50-75 mg/cm3	898:909	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	9	theme	outstanding	974:984	arg1	properties					1006:1015	outstanding mechanical strength properties	974:1015	outstanding mechanical strength properties (as high as 1.37 MPa)	974:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	6	10	theme	rat	1144:1146	arg1	cells					1159:1163	rat osteoblast cells	1144:1163	rat osteoblast cells	1144:1163	Moreover, our study further indicated that these CNCs/HAP materials could increase the proliferation of rat osteoblast cells.					
33736295	5	11	theme	HAP	792:794	arg1	amount					775:780	the amount	771:780	the amount of coated HAP	771:794	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	11	theme	HAP	792:794	arg1	HAP					792:794	coated HAP	785:794	coated HAP	785:794	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	11	theme	HAP	792:794	arg1	crosslinker					804:814	the crosslinker	800:814	the crosslinker	800:814	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	12	with	lightweight	885:895	arg1	absorption					950:959	high water absorption	939:959	high water absorption (>1300%)	939:968	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	12	with	lightweight	885:895	arg1	%					967:967	>1300%	962:967	>1300%	962:967	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	12	with	lightweight	885:895	arg1	properties					1006:1015	outstanding mechanical strength properties	974:1015	outstanding mechanical strength properties (as high as 1.37 MPa)	974:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	13	theme	mechanical	986:995	arg1	properties					1006:1015	outstanding mechanical strength properties	974:1015	outstanding mechanical strength properties (as high as 1.37 MPa)	974:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	0	14	theme	Synergistic	0:10	arg1	enhancement					12:22	Synergistic enhancement	0:22	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.	0:104	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	2	15	theme	entanglements	377:389	arg1	lack					359:362	its lack	355:362	its lack of effective entanglements between the CNCs particles	355:416	However, the application of CNCs-based foams is still challenging, which is attributed to its lack of effective entanglements between the CNCs particles, thus lowering foam properties.					
33736295	5	16	theme	strength	997:1004	arg1	properties					1006:1015	outstanding mechanical strength properties	974:1015	outstanding mechanical strength properties (as high as 1.37 MPa)	974:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	17	theme	foams	870:874	arg1	series					849:854	a series	847:854	a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa)	847:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	7	18	theme	wastewater	1333:1342	arg1	treatment					1344:1352	wastewater treatment	1333:1352	wastewater treatment	1333:1352	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	2	19	theme	effective	367:375	arg1	entanglements					377:389	effective entanglements	367:389	effective entanglements between the CNCs particles	367:416	However, the application of CNCs-based foams is still challenging, which is attributed to its lack of effective entanglements between the CNCs particles, thus lowering foam properties.					
33736295	2	20	theme	foam	433:436	arg1	properties					438:447	foam properties	433:447	foam properties	433:447	However, the application of CNCs-based foams is still challenging, which is attributed to its lack of effective entanglements between the CNCs particles, thus lowering foam properties.					
33736295	7	21	contain	has	1273:1275	arg2	potential					1281:1289	the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications	1277:1404	the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications	1277:1404	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	7	21	contain	has	1273:1275	arg1	foam					1261:1264	improved networked CNCs foam	1237:1264	improved networked CNCs foam	1237:1264	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	3	22	theme	hydroxyapatite	555:568	arg1	layer					576:580	hydroxyapatite (HAP) layer	555:580	hydroxyapatite (HAP) layer onto the CNCs surface	555:602	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	0	23	theme	nanocellulose	27:39	arg1	foam					41:44	nanocellulose foam	27:44	nanocellulose foam	27:44	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	6	24	theme	CNCs/HAP	1089:1096	arg1	materials					1098:1106	these CNCs/HAP materials	1083:1106	these CNCs/HAP materials	1083:1106	Moreover, our study further indicated that these CNCs/HAP materials could increase the proliferation of rat osteoblast cells.					
33736295	2	25	theme	foams	304:308	arg1	application					278:288	the application	274:288	the application of CNCs-based foams	274:308	However, the application of CNCs-based foams is still challenging, which is attributed to its lack of effective entanglements between the CNCs particles, thus lowering foam properties.					
33736295	5	26	theme	CNCs-based	859:868	arg1	foams					870:874	CNCs-based foams	859:874	CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa)	859:1037	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	26	theme	CNCs-based	859:868	arg1	50-75 mg/cm3					898:909	50-75 mg/cm3	898:909	50-75 mg/cm3	898:909	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	27	dep	properties	1006:1015	arg1	high					1021:1024	high	1021:1024	high	1021:1024	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	2	28	theme	CNCs-based	293:302	arg1	foams					304:308	CNCs-based foams	293:308	CNCs-based foams	293:308	However, the application of CNCs-based foams is still challenging, which is attributed to its lack of effective entanglements between the CNCs particles, thus lowering foam properties.					
33736295	5	29	dep	porous	920:925	arg1	%					931:931	~90%	928:931	~90%	928:931	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	3	30	with	mineralization	535:548	arg1	layer					576:580	hydroxyapatite (HAP) layer	555:580	hydroxyapatite (HAP) layer onto the CNCs surface	555:602	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	0	31	dep	in	56:57	arg1	situ					59:62	situ	59:62	situ	59:62	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	7	32	theme	care	1388:1391	arg1	applications					1393:1404	personal care applications	1379:1404	personal care applications	1379:1404	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	2	33	theme	CNCs	403:406	arg1	particles					408:416	the CNCs particles	399:416	the CNCs particles	399:416	However, the application of CNCs-based foams is still challenging, which is attributed to its lack of effective entanglements between the CNCs particles, thus lowering foam properties.					
33736295	0	34	theme	foam	41:44	arg1	enhancement					12:22	Synergistic enhancement	0:22	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.	0:104	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	5	35	theme	~90	928:930	arg1	%					931:931	~90%	928:931	~90%	928:931	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	7	36	theme	CNCs	1256:1259	arg1	foam					1261:1264	improved networked CNCs foam	1237:1264	improved networked CNCs foam	1237:1264	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	5	37	theme	coated	785:790	arg1	HAP					792:794	coated HAP	785:794	coated HAP	785:794	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	0	38	theme	in	56:57	arg1	mineralization					64:77	dual in situ mineralization	51:77	dual in situ mineralization	51:77	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	7	39	theme	networked	1246:1254	arg1	foam					1261:1264	improved networked CNCs foam	1237:1264	improved networked CNCs foam	1237:1264	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	7	40	theme	thermal-retardant	1305:1321	arg1	material					1323:1330	thermal-retardant material	1305:1330	thermal-retardant material	1305:1330	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	3	41	theme	synergistic	467:477	arg1	enhancement					479:489	a synergistic enhancement	465:489	a synergistic enhancement strategy	465:498	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	0	42	theme	dual	51:54	arg1	mineralization					64:77	dual in situ mineralization	51:77	dual in situ mineralization	51:77	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	7	43	theme	personal	1379:1386	arg1	applications					1393:1404	personal care applications	1379:1404	personal care applications	1379:1404	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	3	44	theme	HAP	571:573	arg1	layer					576:580	hydroxyapatite (HAP) layer	555:580	hydroxyapatite (HAP) layer onto the CNCs surface	555:602	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	0	45	with	enhancement	12:22	arg1	mineralization					64:77	dual in situ mineralization	51:77	dual in situ mineralization	51:77	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	0	45	with	enhancement	12:22	arg1	reaction					96:103	crosslinking reaction	83:103	crosslinking reaction	83:103	Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction.					
33736295	3	46	theme	enhancement	479:489	arg1	strategy					491:498	a synergistic enhancement strategy	465:498	a synergistic enhancement strategy	465:498	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	5	47	theme	high	939:942	arg1	absorption					950:959	high water absorption	939:959	high water absorption (>1300%)	939:968	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	5	47	theme	high	939:942	arg1	%					967:967	>1300%	962:967	>1300%	962:967	By controlling the amount of coated HAP and the crosslinker, it is possible to manufacture a series of CNCs-based foams that are lightweight (50-75 mg/cm3), highly porous (~90%) with high water absorption (>1300%) and outstanding mechanical strength properties (as high as 1.37 MPa).					
33736295	3	48	theme	CNCs	591:594	arg1	surface					596:602	the CNCs surface	587:602	the CNCs surface	587:602	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	4	49	theme	composites	695:704	arg1	structures					677:686	The physical and chemical structures	651:686	The physical and chemical structures of the composites	651:704	The physical and chemical structures of the composites were analyzed with SEM, STEM, XRD, FTIR, and TGA.					
33736295	1	50	theme	Cellulose	106:114	arg1	CNCs					130:133	CNCs	130:133	CNCs	130:133	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	1	50	theme	Cellulose	106:114	arg1	nanocrystals					116:127	Cellulose nanocrystals	106:127	Cellulose nanocrystals (CNCs) foams	106:140	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	3	51	theme	chemical	619:626	arg1	reaction					641:648	a chemical crosslinking reaction	617:648	a chemical crosslinking reaction	617:648	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	7	52	theme	improved	1237:1244	arg1	foam					1261:1264	improved networked CNCs foam	1237:1264	improved networked CNCs foam	1237:1264	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	3	53	dep	in	527:528	arg1	situ					530:533	situ	530:533	situ	530:533	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	3	54	theme	crosslinking	628:639	arg1	reaction					641:648	a chemical crosslinking reaction	617:648	a chemical crosslinking reaction	617:648	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	1	55	dep	renewable	199:207	arg1	biodegradable					220:232	biodegradable	220:232	biodegradable	220:232	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	1	55	dep	renewable	199:207	arg1	abundant					210:217	abundant	210:217	abundant	210:217	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	4	56	theme	physical	655:662	arg1	structures					677:686	The physical and chemical structures	651:686	The physical and chemical structures of the composites	651:704	The physical and chemical structures of the composites were analyzed with SEM, STEM, XRD, FTIR, and TGA.					
33736295	7	57	theme	novel	1212:1216	arg1	approach					1218:1225	a novel approach	1210:1225	a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications	1210:1404	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	7	58	theme	tissue	1355:1360	arg1	engineering					1362:1372	tissue engineering	1355:1372	tissue engineering	1355:1372	The method developed in this study presents a novel approach to design improved networked CNCs foam, which has the potential to be used in thermal-retardant material, wastewater treatment, tissue engineering, and personal care applications.					
33736295	3	59	dep	proposed	504:511	arg1	followed					605:612	followed	605:612	followed by a chemical crosslinking reaction	605:648	In this study, a synergistic enhancement strategy was proposed, based on the in situ mineralization with hydroxyapatite (HAP) layer onto the CNCs surface, followed by a chemical crosslinking reaction.					
33736295	1	60	dep	nanocrystals	116:127	arg1	foams					136:140	foams	136:140	foams	136:140	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
33736295	1	61	theme	high	246:249	arg1	area					259:262	high surface area	246:262	high surface area	246:262	Cellulose nanocrystals (CNCs) foams have recently gained research interests because they are renewable, abundant, biodegradable and exhibit high surface area.					
31956958	2	0	dep	s	383:383	arg1	to					376:377	to	376:377	to	376:377	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	0	1	theme	Reducing	85:92	arg1	Sugars					94:99	Reducing Sugars	85:99	Reducing Sugars	85:99	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	1	2	theme	Avicel®	293:299	arg1	substrate					282:290	a cellulosic substrate	269:290	a cellulosic substrate (Avicel®, 2% w.v-1)	269:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	2	theme	Avicel®	293:299	arg1	w.v-1					305:309	Avicel®, 2% w.v-1	293:309	w.v-1	305:309	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	4	3	theme	W	691:691	arg1	cm-2					693:696	180.8 W cm-2	685:696	180.8 W cm-2	685:696	Under optimal conditions (170 s, 180.8 W cm-2, and 25 °C), CMCase activity was 13% higher than that of the control.					
31956958	1	4	theme	ultrasound	155:164	arg1	effect					145:150	the effect	141:150	the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1)	141:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	4	5	theme	CMCase	711:716	arg1	activity					718:725	CMCase activity	711:725	CMCase activity	711:725	Under optimal conditions (170 s, 180.8 W cm-2, and 25 °C), CMCase activity was 13% higher than that of the control.					
31956958	7	6	theme	low-frequency	1098:1110	arg1	ultrasound					1112:1121	a low-frequency ultrasound	1096:1121	a low-frequency ultrasound with a short ultrasonication time of application	1096:1170	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	7	7	theme	second-generation	1041:1057	arg1	production					1067:1076	second-generation ethanol production	1041:1076	second-generation ethanol production	1041:1076	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	7	8	theme	ultrasonication	1136:1150	arg1	time					1152:1155	a short ultrasonication time	1128:1155	a short ultrasonication time of application	1128:1170	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	2	9	theme	W	416:416	arg1	intensity					390:398	US intensity	387:398	US intensity (120.6 to 263.7 W cm-2)	387:422	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	9	theme	W	416:416	arg1	cm-2					418:421	120.6 to 263.7 W cm-2	401:421	120.6 to 263.7 W cm-2	401:421	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	6	10	theme	cellulose	917:925	arg1	%					936:936	42.08%	931:936	42.08%	931:936	The theoretical yield of cellulose was 42.08%.					
31956958	6	10	theme	cellulose	917:925	arg1	yield					908:912	The theoretical yield	892:912	The theoretical yield of cellulose	892:925	The theoretical yield of cellulose was 42.08%.					
31956958	3	11	contain	had	610:612	arg2	effect					625:630	a positive effect	614:630	a positive effect	614:630	US intensity had a positive effect on enzyme activity.					
31956958	3	11	contain	had	610:612	arg1	intensity					600:608	US intensity	597:608	US intensity	597:608	US intensity had a positive effect on enzyme activity.					
31956958	1	12	theme	%	303:303	arg1	substrate					282:290	a cellulosic substrate	269:290	a cellulosic substrate (Avicel®, 2% w.v-1)	269:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	12	theme	%	303:303	arg1	w.v-1					305:309	Avicel®, 2% w.v-1	293:309	w.v-1	305:309	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	2	13	theme	horn-type	484:492	arg1	reactor					505:511	a horn-type ultrasound reactor	482:511	a horn-type ultrasound reactor	482:511	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	1	14	from	effect	145:150	arg1	activity					178:185	the activity	174:185	the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1)	174:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	7	15	theme	application	1160:1170	arg1	time					1152:1155	a short ultrasonication time	1128:1155	a short ultrasonication time of application	1128:1170	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	2	16	theme	endoglucanase	518:530	arg1	activities					569:578	endoglucanase (CMCase) and total cellulase (FPase) activities	518:578	endoglucanase (CMCase) and total cellulase (FPase) activities	518:578	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	17	theme	US	387:388	arg1	intensity					390:398	US intensity	387:398	US intensity (120.6 to 263.7 W cm-2)	387:422	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	17	theme	US	387:388	arg1	cm-2					418:421	120.6 to 263.7 W cm-2	401:421	120.6 to 263.7 W cm-2	401:421	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	6	18	theme	theoretical	896:906	arg1	%					936:936	42.08%	931:936	42.08%	931:936	The theoretical yield of cellulose was 42.08%.					
31956958	6	18	theme	theoretical	896:906	arg1	yield					908:912	The theoretical yield	892:912	The theoretical yield of cellulose	892:925	The theoretical yield of cellulose was 42.08%.					
31956958	7	19	theme	ethanol	1059:1065	arg1	production					1067:1076	second-generation ethanol production	1041:1076	second-generation ethanol production	1041:1076	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	0	20	theme	Low-frequency	0:12	arg1	Ultrasound					14:23	Low-frequency Ultrasound	0:23	Low-frequency Ultrasound with Short Application Time	0:51	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	3	21	theme	US	597:598	arg1	intensity					600:608	US intensity	597:608	US intensity	597:608	US intensity had a positive effect on enzyme activity.					
31956958	1	22	dep	ultrasound	155:164	arg1	US					167:168	US	167:168	US	167:168	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	0	23	theme	Short	30:34	arg1	Time					48:51	Short Application Time	30:51	Short Application Time	30:51	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	2	24	theme	reaction	429:436	arg1	°C					460:461	25 to 50 °C	451:461	25 to 50 °C	451:461	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	24	theme	reaction	429:436	arg1	temperature					438:448	reaction temperature	429:448	reaction temperature (25 to 50 °C)	429:462	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	25	theme	ultrasound	494:503	arg1	reactor					505:511	a horn-type ultrasound reactor	482:511	a horn-type ultrasound reactor	482:511	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	7	26	theme	short	1130:1134	arg1	time					1152:1155	a short ultrasonication time	1128:1155	a short ultrasonication time of application	1128:1170	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	5	27	theme	substrate	787:795	arg1	presence					775:782	the presence	771:782	the presence of substrate	771:795	In the presence of substrate, CMCase activity increased by 33.87% and KM reduced by 23% in relation to that of the control.					
31956958	2	28	theme	Sonication	356:365	arg1	s					383:383	10 to 330 s	373:383	10 to 330 s	373:383	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	28	theme	Sonication	356:365	arg1	time					367:370	Sonication time	356:370	Sonication time (10 to 330 s)	356:384	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	5	29	from	%	854:854	arg1	relation					859:866	relation	859:866	relation to that of the control	859:889	In the presence of substrate, CMCase activity increased by 33.87% and KM reduced by 23% in relation to that of the control.					
31956958	2	30	theme	total	545:549	arg1	cellulase					551:559	total cellulase	545:559	total cellulase (FPase)	545:567	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	30	theme	total	545:549	arg1	FPase					562:566	FPase	562:566	FPase	562:566	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	0	31	dep	Activity	72:79	arg1	Release					101:107	Release	101:107	Release	101:107	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	4	32	dep	conditions	666:675	arg1	cm-2					693:696	180.8 W cm-2	685:696	180.8 W cm-2	685:696	Under optimal conditions (170 s, 180.8 W cm-2, and 25 °C), CMCase activity was 13% higher than that of the control.					
31956958	4	32	dep	conditions	666:675	arg1	s					682:682	170 s	678:682	170 s	678:682	Under optimal conditions (170 s, 180.8 W cm-2, and 25 °C), CMCase activity was 13% higher than that of the control.					
31956958	4	32	dep	conditions	666:675	arg1	°C					706:707	25 °C	703:707	25 °C	703:707	Under optimal conditions (170 s, 180.8 W cm-2, and 25 °C), CMCase activity was 13% higher than that of the control.					
31956958	4	33	theme	optimal	658:664	arg1	conditions					666:675	optimal conditions	658:675	optimal conditions (170 s, 180.8 W cm-2, and 25 °C)	658:708	Under optimal conditions (170 s, 180.8 W cm-2, and 25 °C), CMCase activity was 13% higher than that of the control.					
31956958	7	34	dep	US	977:978	arg1	treatment					980:988	treatment	980:988	US treatment to maximize productivity gains and reduce costs in second-generation ethanol production	977:1076	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	0	35	theme	Application	36:46	arg1	Time					48:51	Short Application Time	30:51	Short Application Time	30:51	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	3	36	theme	positive	616:623	arg1	effect					625:630	a positive effect	614:630	a positive effect	614:630	US intensity had a positive effect on enzyme activity.					
31956958	2	37	dep	50	457:458	arg1	to					454:455	to	454:455	to	454:455	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	1	38	theme	central	320:326	arg1	design					348:353	a central composite rotatable design	318:353	a central composite rotatable design	318:353	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	39	theme	commercial	190:199	arg1	cellulase					201:209	commercial cellulase	190:209	commercial cellulase (Celluclast® 1.5 L)	190:229	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	39	theme	commercial	190:199	arg1	L					228:228	Celluclast® 1.5 L	212:228	Celluclast® 1.5 L	212:228	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	0	40	theme	Cellulase	62:70	arg1	Activity					72:79	Cellulase Activity	62:79	Cellulase Activity	62:79	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	1	41	theme	composite	328:336	arg1	design					348:353	a central composite rotatable design	318:353	a central composite rotatable design	318:353	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	5	42	theme	CMCase	798:803	arg1	activity					805:812	CMCase activity	798:812	CMCase activity	798:812	In the presence of substrate, CMCase activity increased by 33.87% and KM reduced by 23% in relation to that of the control.					
31956958	2	43	theme	cellulase	551:559	arg1	activities					569:578	endoglucanase (CMCase) and total cellulase (FPase) activities	518:578	endoglucanase (CMCase) and total cellulase (FPase) activities	518:578	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	2	44	dep	263.7	410:414	arg1	to					407:408	to	407:408	to	407:408	Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm-2), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined.					
31956958	1	45	theme	rotatable	338:346	arg1	design					348:353	a central composite rotatable design	318:353	a central composite rotatable design	318:353	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	7	46	theme	ultrasound	1112:1121	arg1	action					1086:1091	the action	1082:1091	the action of a low-frequency ultrasound with a short ultrasonication time of application	1082:1170	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	0	47	with	Ultrasound	14:23	arg1	Time					48:51	Short Application Time	30:51	Short Application Time	30:51	Low-frequency Ultrasound with Short Application Time Improves Cellulase Activity and Reducing Sugars Release.					
31956958	1	48	theme	cellulosic	271:280	arg1	substrate					282:290	a cellulosic substrate	269:290	a cellulosic substrate (Avicel®, 2% w.v-1)	269:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	48	theme	cellulosic	271:280	arg1	w.v-1					305:309	Avicel®, 2% w.v-1	293:309	w.v-1	305:309	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	49	from	activity	178:185	arg1	absence					238:244	the absence	234:244	the absence	234:244	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	49	from	activity	178:185	arg1	presence					257:264	the presence	253:264	the presence of a cellulosic substrate (Avicel®, 2% w.v-1)	253:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	7	50	dep	treatment	980:988	arg1	reduce					1025:1030	reduce	1025:1030	reduce costs in second-generation ethanol production	1025:1076	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	7	50	dep	treatment	980:988	arg1	maximize					993:1000	maximize	993:1000	maximize productivity gains	993:1019	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	1	51	theme	cellulase	201:209	arg1	activity					178:185	the activity	174:185	the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1)	174:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	7	52	theme	Cellulase	939:947	arg1	activity					949:956	Cellulase activity	939:956	Cellulase activity	939:956	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	7	53	theme	productivity	1002:1013	arg1	gains					1015:1019	productivity gains	1002:1019	productivity gains	1002:1019	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	7	54	with	ultrasound	1112:1121	arg1	time					1152:1155	a short ultrasonication time	1128:1155	a short ultrasonication time of application	1128:1170	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	7	55	from	costs	1032:1036	arg1	production					1067:1076	second-generation ethanol production	1041:1076	second-generation ethanol production	1041:1076	Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.					
31956958	1	56	theme	Celluclast®	212:222	arg1	cellulase					201:209	commercial cellulase	190:209	commercial cellulase (Celluclast® 1.5 L)	190:229	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	56	theme	Celluclast®	212:222	arg1	L					228:228	Celluclast® 1.5 L	212:228	Celluclast® 1.5 L	212:228	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	1	57	theme	substrate	282:290	arg1	presence					257:264	the presence	253:264	the presence of a cellulosic substrate (Avicel®, 2% w.v-1)	253:310	In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v-1) using a central composite rotatable design.					
31956958	3	58	theme	enzyme	635:640	arg1	activity					642:649	enzyme activity	635:649	enzyme activity	635:649	US intensity had a positive effect on enzyme activity.					
33600144	4	0	theme	controllable	815:826	arg1	way					828:830	a controllable way	813:830	a controllable way	813:830	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	1	1	gly	glycopeptides	152:164	arg2	glycopeptides					152:164	glycopeptides	152:164	glycopeptides	152:164	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	2	2	theme	sample-screening	321:336	arg1	ability					338:344	an effective sample-screening ability	308:344	an effective sample-screening ability to eliminate the interference of nonglycopeptides	308:394	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	4	3	from	group	654:658	arg1	cavities					669:676	their cavities	663:676	their cavities	663:676	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	0	4	theme	Hollow	0:5	arg1	APBA					17:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	4	5	theme	boron	639:643	arg1	group					654:658	the boron hydroxyl group	635:658	the boron hydroxyl group in their cavities	635:676	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	1	6	theme	important	173:181	arg1	measure					192:198	an important clinical measure	170:198	an important clinical measure for the diagnosis of complex diseases	170:236	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	1	6	theme	important	173:181	arg1	detection					139:147	detection	139:147	detection	139:147	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	1	6	theme	important	173:181	arg1	Enrichment					124:133	Enrichment	124:133	Enrichment	124:133	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	4	7	theme	specific	608:615	arg1	area					625:628	large specific surface area	602:628	large specific surface area	602:628	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	5	8	theme	enrichment	897:906	arg1	performance					908:918	an excellent enrichment performance	884:918	an excellent enrichment performance	884:918	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	3	9	theme	magnetic	464:471	arg1	responsiveness					480:493	magnetic and pH responsiveness	464:493	responsiveness	480:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	6	10	theme	serum	1360:1364	arg1	μL					1348:1349	2 μL	1346:1349	2 μL of human serum	1346:1364	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	7	11	theme	MS-based	1487:1494	arg1	glycoproteomics					1496:1510	MS-based glycoproteomics	1487:1510	MS-based glycoproteomics	1487:1510	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	0	12	theme	Glycopeptides	109:121	arg1	Enrichment					84:93	Efficient Enrichment	74:93	Efficient Enrichment of Endogenous Glycopeptides	74:121	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	6	13	from	glycoproteins	1301:1313	arg1	analyses					1334:1341	three replicate analyses	1318:1341	three replicate analyses of 2 μL of human serum	1318:1364	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	6	14	used	used	1195:1198	arg2	HMCAs					1184:1188	the HMCAs	1180:1188	the HMCAs	1180:1188	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	5	15	theme	size-exclusion	988:1001	arg1	effect					1003:1008	perfect size-exclusion effect	980:1008	perfect size-exclusion effect (HRP/BSA, 1:800, w/w)	980:1030	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	7	16	theme	enrichment	1430:1439	arg1	material					1441:1448	a promising enrichment material	1418:1448	a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields	1418:1529	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	7	16	theme	enrichment	1430:1439	arg1	HMCAs					1395:1399	the HMCAs	1391:1399	the HMCAs	1391:1399	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	0	17	theme	Size	39:42	arg1	Exclusion					44:52	Size Exclusion	39:52	Size Exclusion	39:52	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	6	18	theme	complex	1220:1226	arg1	samples					1228:1234	complex samples	1220:1234	complex samples	1220:1234	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	7	19	from	characterization	1467:1482	arg1	glycoproteomics					1496:1510	MS-based glycoproteomics	1487:1510	MS-based glycoproteomics	1487:1510	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	7	19	from	characterization	1467:1482	arg1	fields					1524:1529	related fields	1516:1529	related fields	1516:1529	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	3	20	gly	glycopeptide	513:524	arg2	glycopeptide					513:524	glycopeptide enrichment	513:535	glycopeptide enrichment	513:535	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	2	21	theme	nonglycopeptides	379:394	arg1	interference					363:374	the interference	359:374	the interference of nonglycopeptides	359:394	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	0	22	theme	Efficient	74:82	arg1	Enrichment					84:93	Efficient Enrichment	74:93	Efficient Enrichment of Endogenous Glycopeptides	74:121	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	5	23	theme	favorable	1033:1041	arg1	universality					1043:1054	favorable universality	1033:1054	favorable universality (HRP, IgG, and RNase B)	1033:1078	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	24	theme	detection	939:947	arg1	effect					1003:1008	perfect size-exclusion effect	980:1008	perfect size-exclusion effect (HRP/BSA, 1:800, w/w)	980:1030	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	24	theme	detection	939:947	arg1	limit					930:934	ultralow limit	921:934	ultralow limit of detection (approximately 0.5 fmol μL-1)	921:977	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	24	theme	detection	939:947	arg1	mg/g					1112:1115	150 mg/g	1108:1115	150 mg/g	1108:1115	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	24	theme	detection	939:947	arg1	universality					1043:1054	favorable universality	1033:1054	favorable universality (HRP, IgG, and RNase B)	1033:1078	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	24	theme	detection	939:947	arg1	performance					908:918	an excellent enrichment performance	884:918	an excellent enrichment performance	884:918	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	24	theme	detection	939:947	arg1	capacity					1098:1105	high binding capacity	1085:1105	high binding capacity (150 mg/g)	1085:1116	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	4	25	gly	glycopeptides	768:780	arg2	glycopeptides					768:780	the glycopeptides	764:780	the glycopeptides	764:780	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	6	26	gly	glycoproteins	1301:1313	arg1	glycoproteins					1301:1313	210 glycoproteins	1297:1313	210 glycoproteins in three replicate analyses of 2 μL of human serum	1297:1364	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	3	27	theme	@	433:433	arg1	HMCAs					452:456	HMCAs	452:456	HMCAs	452:456	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	3	27	theme	@	433:433	arg1	nanospheres					439:449	novel hollow MnFe2O4@C@APBA nanospheres	411:449	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	0	28	theme	@	14:14	arg1	APBA					17:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	7	29	theme	related	1516:1522	arg1	fields					1524:1529	related fields	1516:1529	related fields	1516:1529	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	3	30	theme	@	431:431	arg1	C					432:432	novel hollow MnFe2O4@C	411:432	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	0	31	theme	@	16:16	arg1	APBA					17:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	5	32	dep	universality	1043:1054	arg1	HRP					1057:1059	HRP	1057:1059	HRP	1057:1059	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	32	dep	universality	1043:1054	arg1	B					1077:1077	RNase B	1071:1077	RNase B	1071:1077	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	32	dep	universality	1043:1054	arg1	IgG					1062:1064	IgG	1062:1064	IgG	1062:1064	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	2	33	theme	effective	311:319	arg1	ability					338:344	an effective sample-screening ability	308:344	an effective sample-screening ability to eliminate the interference of nonglycopeptides	308:394	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	5	34	theme	RNase	1071:1075	arg1	B					1077:1077	RNase B	1071:1077	RNase B	1071:1077	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	35	theme	perfect	980:986	arg1	effect					1003:1008	perfect size-exclusion effect	980:1008	perfect size-exclusion effect (HRP/BSA, 1:800, w/w)	980:1030	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	6	36	dep	analyses	1334:1341	arg1	replicate					1324:1332	replicate	1324:1332	replicate	1324:1332	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	6	37	theme	μL	1348:1349	arg1	analyses					1334:1341	three replicate analyses	1318:1341	three replicate analyses of 2 μL of human serum	1318:1364	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	4	38	theme	suitable	572:579	arg1	structure					588:596	a suitable hollow structure	570:596	a suitable hollow structure	570:596	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	1	39	theme	complex	221:227	arg1	diseases					229:236	complex diseases	221:236	complex diseases	221:236	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	4	40	gly	glycopeptides	730:742	arg2	glycopeptides					730:742	the glycopeptides	726:742	the glycopeptides	726:742	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	5	41	theme	binding	1090:1096	arg1	capacity					1098:1105	high binding capacity	1085:1105	high binding capacity (150 mg/g)	1085:1116	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	41	theme	binding	1090:1096	arg1	mg/g					1112:1115	150 mg/g	1108:1115	150 mg/g	1108:1115	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	4	42	theme	as-prepared	542:552	arg1	composites					554:563	The as-prepared composites	538:563	The as-prepared composites	538:563	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	5	43	dep	effect	1003:1008	arg1	1:800					1020:1024	1:800	1020:1024	1:800	1020:1024	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	43	dep	effect	1003:1008	arg1	HRP/BSA					1011:1017	HRP/BSA	1011:1017	HRP/BSA	1011:1017	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	43	dep	effect	1003:1008	arg1	w/w					1027:1029	w/w	1027:1029	w/w	1027:1029	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	3	44	with	nanospheres	439:449	arg1	responsiveness					480:493	magnetic and pH responsiveness	464:493	responsiveness	480:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	2	45	theme	key	267:269	arg1	role					271:274	a key role	265:274	a key role	265:274	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	1	46	theme	glycopeptides	152:164	arg1	measure					192:198	an important clinical measure	170:198	an important clinical measure for the diagnosis of complex diseases	170:236	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	1	46	theme	glycopeptides	152:164	arg1	detection					139:147	detection	139:147	detection	139:147	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	1	46	theme	glycopeptides	152:164	arg1	Enrichment					124:133	Enrichment	124:133	Enrichment	124:133	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	0	47	theme	Endogenous	98:107	arg1	Glycopeptides					109:121	Endogenous Glycopeptides	98:121	Endogenous Glycopeptides	98:121	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	3	48	theme	glycopeptide	513:524	arg1	enrichment					526:535	glycopeptide enrichment	513:535	glycopeptide enrichment	513:535	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	4	49	theme	large	602:606	arg1	area					625:628	large specific surface area	602:628	large specific surface area	602:628	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	4	50	contain	have	565:568	arg2	structure					588:596	a suitable hollow structure	570:596	a suitable hollow structure	570:596	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	4	50	contain	have	565:568	arg2	area					625:628	large specific surface area	602:628	large specific surface area	602:628	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	4	50	contain	have	565:568	arg1	composites					554:563	The as-prepared composites	538:563	The as-prepared composites	538:563	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	6	51	theme	human	1354:1358	arg1	serum					1360:1364	human serum	1354:1364	human serum	1354:1364	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	7	52	theme	glycopeptide	1454:1465	arg1	characterization					1467:1482	glycopeptide characterization	1454:1482	glycopeptide characterization in MS-based glycoproteomics and related fields	1454:1529	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	4	53	theme	surface	617:623	arg1	area					625:628	large specific surface area	602:628	large specific surface area	602:628	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	7	54	theme	promising	1420:1428	arg1	material					1441:1448	a promising enrichment material	1418:1448	a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields	1418:1529	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	7	54	theme	promising	1420:1428	arg1	HMCAs					1395:1399	the HMCAs	1391:1399	the HMCAs	1391:1399	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	6	55	gly	glycopeptides	1262:1274	arg2	glycopeptides					1262:1274	474 glycopeptides	1258:1274	474 glycopeptides	1258:1274	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	5	56	dep	limit	930:934	arg1	fmol					968:971	approximately 0.5 fmol	950:971	approximately 0.5 fmol μL-1	950:976	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	2	57	theme	Enrichment	239:248	arg1	materials					250:258	Enrichment materials	239:258	Enrichment materials	239:258	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	5	58	theme	Enrichment	833:842	arg1	results					844:850	Enrichment results	833:850	Enrichment results	833:850	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	4	59	theme	hydroxyl	645:652	arg1	group					654:658	the boron hydroxyl group	635:658	the boron hydroxyl group in their cavities	635:676	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	0	60	theme	pH	58:59	arg1	Response					61:68	pH Response	58:68	pH Response	58:68	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	2	61	contain	have	303:306	arg2	ability					338:344	an effective sample-screening ability	308:344	an effective sample-screening ability to eliminate the interference of nonglycopeptides	308:394	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	2	61	contain	have	303:306	arg1	they					293:296	they	293:296	they	293:296	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	6	62	theme	samples	1228:1234	arg1	analysis					1208:1215	the analysis	1204:1215	the analysis of complex samples	1204:1234	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	5	63	theme	ultralow	921:928	arg1	limit					930:934	ultralow limit	921:934	ultralow limit of detection (approximately 0.5 fmol μL-1)	921:977	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	64	dep	fmol	968:971	arg1	μL-1					973:976	μL-1	973:976	approximately 0.5 fmol μL-1	950:976	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	7	65	used	used	1410:1413	arg2	HMCAs					1395:1399	the HMCAs	1391:1399	the HMCAs	1391:1399	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	7	65	used	used	1410:1413	arg2	material					1441:1448	a promising enrichment material	1418:1448	a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields	1418:1529	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	2	66	gly	nonglycopeptides	379:394	arg2	nonglycopeptides					379:394	nonglycopeptides	379:394	nonglycopeptides	379:394	Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of nonglycopeptides.					
33600144	4	67	theme	glycopeptides	730:742	arg1	groups					716:721	the hydrophilic groups	700:721	the hydrophilic groups of the glycopeptides	700:742	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	4	67	theme	glycopeptides	730:742	arg1	glycopeptides					730:742	the glycopeptides	726:742	the glycopeptides	726:742	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	3	68	theme	MnFe2O4	424:430	arg1	C					432:432	novel hollow MnFe2O4@C	411:432	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	0	69	theme	MnFe2O4	7:13	arg1	APBA					17:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	5	70	theme	excellent	887:895	arg1	performance					908:918	an excellent enrichment performance	884:918	an excellent enrichment performance	884:918	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	3	71	theme	novel	411:415	arg1	C					432:432	novel hollow MnFe2O4@C	411:432	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	0	72	theme	C	15:15	arg1	APBA					17:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA	0:20	Hollow MnFe2O4@C@APBA Nanospheres with Size Exclusion and pH Response for Efficient Enrichment of Endogenous Glycopeptides.					
33600144	4	73	theme	hydrophilic	704:714	arg1	groups					716:721	the hydrophilic groups	700:721	the hydrophilic groups of the glycopeptides	700:742	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	4	73	theme	hydrophilic	704:714	arg1	glycopeptides					730:742	the glycopeptides	726:742	the glycopeptides	726:742	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	3	74	theme	APBA	434:437	arg1	HMCAs					452:456	HMCAs	452:456	HMCAs	452:456	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	3	74	theme	APBA	434:437	arg1	nanospheres					439:449	novel hollow MnFe2O4@C@APBA nanospheres	411:449	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	5	75	theme	high	1085:1088	arg1	capacity					1098:1105	high binding capacity	1085:1105	high binding capacity (150 mg/g)	1085:1116	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	5	75	theme	high	1085:1088	arg1	mg/g					1112:1115	150 mg/g	1108:1115	150 mg/g	1108:1115	Enrichment results showed that the HMCAs exhibited an excellent enrichment performance: ultralow limit of detection (approximately 0.5 fmol μL-1), perfect size-exclusion effect (HRP/BSA, 1:800, w/w), favorable universality (HRP, IgG, and RNase B), and high binding capacity (150 mg/g).					
33600144	3	76	theme	hollow	417:422	arg1	C					432:432	novel hollow MnFe2O4@C	411:432	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	1	77	dep	important	173:181	arg1	clinical					183:190	clinical	183:190	clinical	183:190	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	3	78	theme	C	432:432	arg1	HMCAs					452:456	HMCAs	452:456	HMCAs	452:456	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	3	78	theme	C	432:432	arg1	nanospheres					439:449	novel hollow MnFe2O4@C@APBA nanospheres	411:449	novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness	411:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	6	79	from	practice	1170:1177	arg1	application					1142:1152	the application	1138:1152	the application of materials in practice	1138:1177	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	7	80	gly	glycopeptide	1454:1465	arg2	glycopeptide					1454:1465	glycopeptide	1454:1465	glycopeptide	1454:1465	The results showed that the HMCAs could be used as a promising enrichment material for glycopeptide characterization in MS-based glycoproteomics and related fields.					
33600144	6	81	theme	materials	1157:1165	arg1	application					1142:1152	the application	1138:1152	the application of materials in practice	1138:1177	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	4	82	theme	hollow	581:586	arg1	structure					588:596	a suitable hollow structure	570:596	a suitable hollow structure	570:596	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	4	83	theme	different	747:755	arg1	pH					757:758	different pH	747:758	different pH	747:758	The as-prepared composites have a suitable hollow structure and large specific surface area, and the boron hydroxyl group in their cavities can fix or disconnect the hydrophilic groups of the glycopeptides at different pH, so the glycopeptides can be adsorbed or desorbed in a controllable way.					
33600144	6	84	from	application	1142:1152	arg1	practice					1170:1177	practice	1170:1177	practice	1170:1177	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
33600144	1	85	theme	diseases	229:236	arg1	diagnosis					208:216	the diagnosis	204:216	the diagnosis of complex diseases	204:236	Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases.					
33600144	3	86	theme	pH	477:478	arg1	responsiveness					480:493	magnetic and pH responsiveness	464:493	responsiveness	480:493	In this work, novel hollow MnFe2O4@C@APBA nanospheres (HMCAs) with magnetic and pH responsiveness were prepared for glycopeptide enrichment.					
33600144	6	87	from	materials	1157:1165	arg1	practice					1170:1177	practice	1170:1177	practice	1170:1177	In order to verify the application of materials in practice, the HMCAs were used for the analysis of complex samples and it was found that 474 glycopeptides were identified from 210 glycoproteins in three replicate analyses of 2 μL of human serum.					
34229015	7	0	theme	adsorption	1228:1237	arg1	process					1239:1245	the adsorption process	1224:1245	the adsorption process	1224:1245	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	1	1	theme	scanning	345:352	arg1	SEM					375:377	SEM	375:377	SEM	375:377	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	1	theme	scanning	345:352	arg1	microscopy					363:372	scanning electron microscopy	345:372	scanning electron microscopy (SEM)	345:378	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	10	2	theme	investigated	1483:1494	arg1	compounds					1505:1513	All investigated phenolic compounds	1479:1513	All investigated phenolic compounds	1479:1513	All investigated phenolic compounds showed the higher conversion as catalyzed by IPPO-Au.					
34229015	9	3	theme	immobilized	1422:1432	arg1	PPO					1434:1436	The immobilized PPO	1418:1436	The immobilized PPO	1418:1436	The immobilized PPO were used to oxidize phenolic compounds.					
34229015	8	4	from	range	1411:1415	arg1	exothermic					1342:1351	exothermic	1342:1351	exothermic	1342:1351	The adsorption of PPO was exothermic, physical and spontaneous at the investigated temperature range.					
34229015	8	4	from	range	1411:1415	arg1	adsorption					1320:1329	The adsorption	1316:1329	The adsorption of PPO	1316:1336	The adsorption of PPO was exothermic, physical and spontaneous at the investigated temperature range.					
34229015	9	5	used	used	1443:1446	arg2	PPO					1434:1436	The immobilized PPO	1418:1436	The immobilized PPO	1418:1436	The immobilized PPO were used to oxidize phenolic compounds.					
34229015	1	6	theme	electron	354:361	arg1	SEM					375:377	SEM	375:377	SEM	375:377	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	6	theme	electron	354:361	arg1	microscopy					363:372	scanning electron microscopy	345:372	scanning electron microscopy (SEM)	345:378	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	7	theme	oxidase	438:444	arg1	immobilization					409:422	immobilization	409:422	immobilization of polyphenol oxidase (PPO)	409:450	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	8	theme	chitosan‑gold	166:178	arg1	CTS-Au/MMT					211:220	CTS-Au/MMT	211:220	CTS-Au/MMT	211:220	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	8	theme	chitosan‑gold	166:178	arg1	nanoparticles/montmorillonite					180:208	chitosan‑gold nanoparticles/montmorillonite	166:208	chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT)	166:221	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	9	used	utilized	385:392	arg2	support					397:403	support	397:403	support for immobilization of polyphenol oxidase (PPO)	397:450	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	9	used	utilized	385:392	arg2	composites					223:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites	127:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites	127:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	7	10	theme	only	1288:1291	arg1	diffusion					1266:1274	intra-particle diffusion	1251:1274	intra-particle diffusion	1251:1274	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	7	10	theme	only	1288:1291	arg1	step					1310:1313	the only rate-controlling step	1284:1313	the only rate-controlling step	1284:1313	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	3	11	theme	analysis	730:737	arg1	approach					746:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	7	12	theme	rate-controlling	1293:1308	arg1	diffusion					1266:1274	intra-particle diffusion	1251:1274	intra-particle diffusion	1251:1274	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	7	12	theme	rate-controlling	1293:1308	arg1	step					1310:1313	the only rate-controlling step	1284:1313	the only rate-controlling step	1284:1313	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	0	13	theme	phenolic	107:114	arg1	compounds					116:124	phenolic compounds	107:124	phenolic compounds	107:124	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	4	14	theme	immobilization	774:787	arg1	condition					789:797	the optimal immobilization condition	762:797	the optimal immobilization condition	762:797	Under the optimal immobilization condition, the immobilization efficiency and enzyme activity reached at 50.16% and 1.46 × 104 U/mg for IPPO, and 63.35% and 3.01 × 104 U/mg for IPPO-Au, respectively.					
34229015	1	15	theme	nanoparticles/montmorillonite	180:208	arg1	support					397:403	support	397:403	support for immobilization of polyphenol oxidase (PPO)	397:450	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	15	theme	nanoparticles/montmorillonite	180:208	arg1	composites					223:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites	127:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites	127:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	9	16	theme	phenolic	1459:1466	arg1	compounds					1468:1476	phenolic compounds	1459:1476	phenolic compounds	1459:1476	The immobilized PPO were used to oxidize phenolic compounds.					
34229015	3	17	theme	Taguchi-Grey	706:717	arg1	approach					746:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	4	18	theme	immobilization	804:817	arg1	efficiency					819:828	the immobilization efficiency	800:828	the immobilization efficiency	800:828	Under the optimal immobilization condition, the immobilization efficiency and enzyme activity reached at 50.16% and 1.46 × 104 U/mg for IPPO, and 63.35% and 3.01 × 104 U/mg for IPPO-Au, respectively.					
34229015	4	19	theme	optimal	766:772	arg1	condition					789:797	the optimal immobilization condition	762:797	the optimal immobilization condition	762:797	Under the optimal immobilization condition, the immobilization efficiency and enzyme activity reached at 50.16% and 1.46 × 104 U/mg for IPPO, and 63.35% and 3.01 × 104 U/mg for IPPO-Au, respectively.					
34229015	7	20	theme	film	1192:1195	arg1	diffusion					1197:1205	film diffusion	1192:1205	film diffusion	1192:1205	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	3	21	theme	relational	719:728	arg1	approach					746:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	5	22	theme	PPO	1000:1002	arg1	adsorption					1004:1013	PPO adsorption	1000:1013	PPO adsorption	1000:1013	The isotherm, kinetic and thermodynamics of PPO adsorption were investigated in detail.					
34229015	8	23	theme	temperature	1399:1409	arg1	range					1411:1415	the investigated temperature range	1382:1415	the investigated temperature range	1382:1415	The adsorption of PPO was exothermic, physical and spontaneous at the investigated temperature range.					
34229015	0	24	theme	Process	0:6	arg1	optimization					8:19	Process optimization	0:19	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.	0:125	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	10	25	theme	phenolic	1496:1503	arg1	compounds					1505:1513	All investigated phenolic compounds	1479:1513	All investigated phenolic compounds	1479:1513	All investigated phenolic compounds showed the higher conversion as catalyzed by IPPO-Au.					
34229015	8	26	theme	investigated	1386:1397	arg1	range					1411:1415	the investigated temperature range	1382:1415	the investigated temperature range	1382:1415	The adsorption of PPO was exothermic, physical and spontaneous at the investigated temperature range.					
34229015	0	27	theme	polyphenol	24:33	arg1	immobilization					43:56	polyphenol oxidase immobilization	24:56	polyphenol oxidase immobilization	24:56	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	1	28	theme	X-ray	309:313	arg1	XRD					335:337	XRD	335:337	XRD	335:337	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	28	theme	X-ray	309:313	arg1	diffraction					322:332	X-ray powder diffraction	309:332	X-ray powder diffraction (XRD)	309:338	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	2	29	theme	physical	519:526	arg1	adsorption					528:537	physical adsorption	519:537	physical adsorption	519:537	PPO was immobilized on CTS/MMT (IPPO) and CTS-Au/MMT (IPPO-Au) by physical adsorption, respectively.					
34229015	0	30	dep	kinetic	69:75	arg1	thermodynamic					78:90	thermodynamic	78:90	thermodynamic	78:90	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	1	31	theme	powder	315:320	arg1	XRD					335:337	XRD	335:337	XRD	335:337	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	31	theme	powder	315:320	arg1	diffraction					322:332	X-ray powder diffraction	309:332	X-ray powder diffraction (XRD)	309:338	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	0	32	theme	compounds	116:124	arg1	removal					96:102	removal	96:102	removal of phenolic compounds	96:124	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	0	32	theme	compounds	116:124	arg1	Isotherm					59:66	Isotherm	59:66	Isotherm	59:66	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	3	33	theme	enzyme	633:638	arg1	activity					640:647	enzyme activity	633:647	enzyme activity	633:647	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	6	34	theme	Toth	1091:1094	arg1	isotherm					1096:1103	Toth isotherm	1091:1103	Toth isotherm	1091:1103	The adsorption process was better explained by Toth isotherm and Fractal-like pseudo second order model, respectively.					
34229015	6	35	theme	adsorption	1048:1057	arg1	process					1059:1065	The adsorption process	1044:1065	The adsorption process	1044:1065	The adsorption process was better explained by Toth isotherm and Fractal-like pseudo second order model, respectively.					
34229015	0	36	theme	immobilization	43:56	arg1	optimization					8:19	Process optimization	0:19	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.	0:125	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	3	37	theme	activity	640:647	arg1	maximization					587:598	simultaneous maximization	574:598	simultaneous maximization of immobilization efficiency and enzyme activity	574:647	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	8	38	theme	PPO	1334:1336	arg1	exothermic					1342:1351	exothermic	1342:1351	exothermic	1342:1351	The adsorption of PPO was exothermic, physical and spontaneous at the investigated temperature range.					
34229015	8	38	theme	PPO	1334:1336	arg1	adsorption					1320:1329	The adsorption	1316:1329	The adsorption of PPO	1316:1336	The adsorption of PPO was exothermic, physical and spontaneous at the investigated temperature range.					
34229015	0	39	theme	oxidase	35:41	arg1	immobilization					43:56	polyphenol oxidase immobilization	24:56	polyphenol oxidase immobilization	24:56	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	7	40	theme	Intra-particle	1163:1176	arg1	diffusion					1178:1186	Intra-particle diffusion	1163:1186	Intra-particle diffusion	1163:1186	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	3	41	theme	efficiency	618:627	arg1	maximization					587:598	simultaneous maximization	574:598	simultaneous maximization of immobilization efficiency and enzyme activity	574:647	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	10	42	theme	higher	1526:1531	arg1	conversion					1533:1542	the higher conversion	1522:1542	the higher conversion as catalyzed by IPPO-Au	1522:1566	All investigated phenolic compounds showed the higher conversion as catalyzed by IPPO-Au.					
34229015	3	43	theme	immobilization	654:667	arg1	process					669:675	the immobilization process	650:675	the immobilization process parameters	650:686	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	4	44	theme	enzyme	834:839	arg1	activity					841:848	enzyme activity	834:848	enzyme activity	834:848	Under the optimal immobilization condition, the immobilization efficiency and enzyme activity reached at 50.16% and 1.46 × 104 U/mg for IPPO, and 63.35% and 3.01 × 104 U/mg for IPPO-Au, respectively.					
34229015	11	45	theme	substituted	1614:1624	arg1	phenols					1626:1632	substituted phenols	1614:1632	substituted phenols	1614:1632	For both IPPO and IPPO-Au, the conversion of substituted phenols was higher than that of phenol.					
34229015	6	46	theme	order	1136:1140	arg1	model					1142:1146	Fractal-like pseudo second order model	1109:1146	Fractal-like pseudo second order model	1109:1146	The adsorption process was better explained by Toth isotherm and Fractal-like pseudo second order model, respectively.					
34229015	3	47	theme	process	669:675	arg1	parameters					677:686	the immobilization process parameters	650:686	the immobilization process parameters	650:686	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	11	48	theme	phenols	1626:1632	arg1	higher					1638:1643	higher	1638:1643	higher	1638:1643	For both IPPO and IPPO-Au, the conversion of substituted phenols was higher than that of phenol.					
34229015	11	48	theme	phenols	1626:1632	arg1	conversion					1600:1609	the conversion	1596:1609	the conversion of substituted phenols	1596:1632	For both IPPO and IPPO-Au, the conversion of substituted phenols was higher than that of phenol.					
34229015	1	49	theme	Chitosan/montmorillonite	127:150	arg1	support					397:403	support	397:403	support for immobilization of polyphenol oxidase (PPO)	397:450	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	49	theme	Chitosan/montmorillonite	127:150	arg1	composites					223:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites	127:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites	127:232	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	3	50	theme	simultaneous	574:585	arg1	maximization					587:598	simultaneous maximization	574:598	simultaneous maximization of immobilization efficiency and enzyme activity	574:647	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	3	51	theme	immobilization	603:616	arg1	efficiency					618:627	immobilization efficiency	603:627	immobilization efficiency	603:627	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	6	52	theme	pseudo	1122:1127	arg1	model					1142:1146	Fractal-like pseudo second order model	1109:1146	Fractal-like pseudo second order model	1109:1146	The adsorption process was better explained by Toth isotherm and Fractal-like pseudo second order model, respectively.					
34229015	3	53	theme	TGRA	740:743	arg1	approach					746:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	Taguchi-Grey relational analysis (TGRA) approach	706:753	In order to achieve simultaneous maximization of immobilization efficiency and enzyme activity, the immobilization process parameters were optimized by Taguchi-Grey relational analysis (TGRA) approach.					
34229015	6	54	theme	second	1129:1134	arg1	model					1142:1146	Fractal-like pseudo second order model	1109:1146	Fractal-like pseudo second order model	1109:1146	The adsorption process was better explained by Toth isotherm and Fractal-like pseudo second order model, respectively.					
34229015	0	55	theme	kinetic	69:75	arg1	Isotherm					59:66	Isotherm	59:66	Isotherm	59:66	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	1	56	theme	polyphenol	427:436	arg1	PPO					447:449	PPO	447:449	PPO	447:449	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	56	theme	polyphenol	427:436	arg1	oxidase					438:444	polyphenol oxidase	427:444	polyphenol oxidase (PPO)	427:450	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	0	57	dep	optimization	8:19	arg1	removal					96:102	removal	96:102	removal of phenolic compounds	96:124	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	0	57	dep	optimization	8:19	arg1	Isotherm					59:66	Isotherm	59:66	Isotherm	59:66	Process optimization of polyphenol oxidase immobilization: Isotherm, kinetic, thermodynamic and removal of phenolic compounds.					
34229015	6	58	theme	Fractal-like	1109:1120	arg1	model					1142:1146	Fractal-like pseudo second order model	1109:1146	Fractal-like pseudo second order model	1109:1146	The adsorption process was better explained by Toth isotherm and Fractal-like pseudo second order model, respectively.					
34229015	1	59	theme	Fourier	271:277	arg1	FT-IR					301:305	FT-IR	301:305	FT-IR	301:305	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	59	theme	Fourier	271:277	arg1	infrared					291:298	Fourier transformed infrared	271:298	Fourier transformed infrared (FT-IR)	271:306	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	7	60	theme	intra-particle	1251:1264	arg1	diffusion					1266:1274	intra-particle diffusion	1251:1274	intra-particle diffusion	1251:1274	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	7	60	theme	intra-particle	1251:1264	arg1	step					1310:1313	the only rate-controlling step	1284:1313	the only rate-controlling step	1284:1313	Intra-particle diffusion and film diffusion were involved in the adsorption process and intra-particle diffusion was not the only rate-controlling step.					
34229015	5	61	theme	adsorption	1004:1013	arg1	thermodynamics					982:995	thermodynamics	982:995	thermodynamics	982:995	The isotherm, kinetic and thermodynamics of PPO adsorption were investigated in detail.					
34229015	5	61	theme	adsorption	1004:1013	arg1	isotherm					960:967	The isotherm	956:967	The isotherm	956:967	The isotherm, kinetic and thermodynamics of PPO adsorption were investigated in detail.					
34229015	1	62	theme	transformed	279:289	arg1	FT-IR					301:305	FT-IR	301:305	FT-IR	301:305	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
34229015	1	62	theme	transformed	279:289	arg1	infrared					291:298	Fourier transformed infrared	271:298	Fourier transformed infrared (FT-IR)	271:306	Chitosan/montmorillonite (CTS/MMT) and chitosan‑gold nanoparticles/montmorillonite (CTS-Au/MMT) composites were prepared, characterized through Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM), and utilized as support for immobilization of polyphenol oxidase (PPO).					
32526650	4	0	contain	possessed	721:729	arg2	performance					751:761	superior adsorption performance	731:761	superior adsorption performance	731:761	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	4	0	contain	possessed	721:729	arg1	CS/MOF-SH					702:710	CS/MOF-SH	702:710	CS/MOF-SH	702:710	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	5	1	theme	trace	1080:1084	arg1	ions					1098:1101	trace heavy metal ions	1080:1101	trace heavy metal ions in real food samples	1080:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	2	theme	real	1106:1109	arg1	samples					1116:1122	real food samples	1106:1122	real food samples	1106:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	0	3	theme	lead	102:105	arg1	ions					119:122	cadmium ions	111:122	cadmium ions in food samples	111:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	0	3	theme	lead	102:105	arg1	determination					85:97	the simultaneous determination	68:97	the simultaneous determination of lead	68:105	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	5	4	theme	heavy	1086:1090	arg1	ions					1098:1101	trace heavy metal ions	1080:1101	trace heavy metal ions in real food samples	1080:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	3	5	theme	0.033 µg	630:637	arg1	L-1					639:641	0.033 µg L-1 and 0.008 µg L-1	630:658	L-1	639:641	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	3	5	theme	0.033 µg	630:637	arg1	limits					601:606	the detection limits	587:606	the detection limits of Pb2+ and Cd2+	587:623	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	0	6	from	determination	85:97	arg1	samples					132:138	food samples	127:138	food samples	127:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	5	7	from	Cd2+	921:924	arg1	wheat					974:978	wheat	974:978	wheat	974:978	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	7	from	Cd2+	921:924	arg1	rice					968:971	rice	968:971	rice	968:971	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	7	from	Cd2+	921:924	arg1	materials					957:965	various certified reference materials	929:965	various certified reference materials (rice, wheat and tea) with complicated matrices	929:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	7	from	Cd2+	921:924	arg1	tea					984:986	tea	984:986	tea	984:986	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	1	8	theme	trace	234:238	arg1	Pb2+					240:243	trace Pb2+	234:243	trace Pb2+	234:243	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	2	9	theme	extraction	505:514	arg1	time					516:519	extraction time	505:519	extraction time	505:519	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	5	10	theme	metal	1092:1096	arg1	ions					1098:1101	trace heavy metal ions	1080:1101	trace heavy metal ions in real food samples	1080:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	3	11	theme	Cd2+	620:623	arg1	L-1					639:641	0.033 µg L-1 and 0.008 µg L-1	630:658	L-1	639:641	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	3	11	theme	Cd2+	620:623	arg1	limits					601:606	the detection limits	587:606	the detection limits of Pb2+ and Cd2+	587:623	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	4	12	theme	ease	795:798	arg1	advantages					781:790	the advantages	777:790	the advantages of ease of handling and recyclability	777:828	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	4	13	theme	superior	731:738	arg1	performance					751:761	superior adsorption performance	731:761	superior adsorption performance	731:761	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	1	14	theme	Pb2+	240:243	arg1	analysis					222:229	the analysis	218:229	the analysis of trace Pb2+ and Cd2+	218:252	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	0	15	theme	cadmium	111:117	arg1	ions					119:122	cadmium ions	111:122	cadmium ions in food samples	111:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	5	16	with	materials	957:965	arg1	matrices					1006:1013	complicated matrices	994:1013	complicated matrices	994:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	0	17	theme	food	127:130	arg1	samples					132:138	food samples	127:138	food samples	127:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	4	18	theme	handling	803:810	arg1	ease					795:798	ease	795:798	ease of handling and recyclability	795:828	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	5	19	theme	various	929:935	arg1	wheat					974:978	wheat	974:978	wheat	974:978	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	19	theme	various	929:935	arg1	rice					968:971	rice	968:971	rice	968:971	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	19	theme	various	929:935	arg1	materials					957:965	various certified reference materials	929:965	various certified reference materials (rice, wheat and tea) with complicated matrices	929:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	19	theme	various	929:935	arg1	tea					984:986	tea	984:986	tea	984:986	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	1	20	theme	solid	164:168	arg1	method					193:198	a facile solid phase extraction (SPE) method	155:198	a facile solid phase extraction (SPE) method	155:198	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	2	21	theme	solution	470:477	arg1	pH					479:480	solution pH	470:480	solution pH	470:480	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	4	22	theme	adsorption	740:749	arg1	performance					751:761	superior adsorption performance	731:761	superior adsorption performance	731:761	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	1	23	theme	phase	170:174	arg1	method					193:198	a facile solid phase extraction (SPE) method	155:198	a facile solid phase extraction (SPE) method	155:198	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	2	24	theme	influencing	441:451	arg1	time					516:519	extraction time	505:519	extraction time	505:519	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	2	24	theme	influencing	441:451	arg1	dosage					493:498	adsorbent dosage	483:498	adsorbent dosage	483:498	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	2	24	theme	influencing	441:451	arg1	pH					479:480	solution pH	470:480	solution pH	470:480	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	2	24	theme	influencing	441:451	arg1	factors					453:459	The potential influencing factors	427:459	The potential influencing factors	427:459	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	1	25	theme	graphite	359:366	arg1	detection					416:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	4	26	theme	recyclability	816:828	arg1	ease					795:798	ease	795:798	ease of handling and recyclability	795:828	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	1	27	theme	extraction	176:185	arg1	method					193:198	a facile solid phase extraction (SPE) method	155:198	a facile solid phase extraction (SPE) method	155:198	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	2	28	theme	potential	431:439	arg1	time					516:519	extraction time	505:519	extraction time	505:519	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	2	28	theme	potential	431:439	arg1	dosage					493:498	adsorbent dosage	483:498	adsorbent dosage	483:498	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	2	28	theme	potential	431:439	arg1	pH					479:480	solution pH	470:480	solution pH	470:480	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	2	28	theme	potential	431:439	arg1	factors					453:459	The potential influencing factors	427:459	The potential influencing factors	427:459	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	1	29	theme	Cd2+	249:252	arg1	analysis					222:229	the analysis	218:229	the analysis of trace Pb2+ and Cd2+	218:252	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	5	30	theme	trace	906:910	arg1	Pb2+					912:915	trace Pb2+	906:915	trace Pb2+	906:915	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	31	theme	practical	1034:1042	arg1	potential					1044:1052	its practical potential	1030:1052	its practical potential for regular monitoring of trace heavy metal ions in real food samples	1030:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	3	32	theme	extraction	564:573	arg1	conditions					575:584	the optimized extraction conditions	550:584	the optimized extraction conditions	550:584	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	1	33	theme	SPE	188:190	arg1	method					193:198	a facile solid phase extraction (SPE) method	155:198	a facile solid phase extraction (SPE) method	155:198	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	0	34	theme	metal-organic	30:42	arg1	framework					44:52	metal-organic framework composite	30:62	metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples	30:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	1	35	theme	furnace	368:374	arg1	detection					416:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	36	theme	facile	157:162	arg1	method					193:198	a facile solid phase extraction (SPE) method	155:198	a facile solid phase extraction (SPE) method	155:198	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	37	theme	chitosan/thiol	263:276	arg1	CS/MOF-SH					313:321	CS/MOF-SH	313:321	CS/MOF-SH	313:321	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	37	theme	chitosan/thiol	263:276	arg1	frameworks					301:310	chitosan/thiol modified metal-organic frameworks	263:310	chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite	263:332	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	38	theme	atomic	376:381	arg1	detection					416:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	0	39	theme	composite	54:62	arg1	framework					44:52	metal-organic framework composite	30:62	metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples	30:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	1	40	theme	modified	278:285	arg1	CS/MOF-SH					313:321	CS/MOF-SH	313:321	CS/MOF-SH	313:321	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	40	theme	modified	278:285	arg1	frameworks					301:310	chitosan/thiol modified metal-organic frameworks	263:310	chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite	263:332	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	41	theme	absorption	383:392	arg1	detection					416:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	5	42	dep	materials	957:965	arg1	wheat					974:978	wheat	974:978	wheat	974:978	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	42	dep	materials	957:965	arg1	rice					968:971	rice	968:971	rice	968:971	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	42	dep	materials	957:965	arg1	materials					957:965	various certified reference materials	929:965	various certified reference materials (rice, wheat and tea) with complicated matrices	929:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	42	dep	materials	957:965	arg1	tea					984:986	tea	984:986	tea	984:986	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	0	43	theme	simultaneous	72:83	arg1	determination					85:97	the simultaneous determination	68:97	the simultaneous determination of lead	68:105	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	1	44	theme	metal-organic	287:299	arg1	CS/MOF-SH					313:321	CS/MOF-SH	313:321	CS/MOF-SH	313:321	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	44	theme	metal-organic	287:299	arg1	frameworks					301:310	chitosan/thiol modified metal-organic frameworks	263:310	chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite	263:332	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	1	45	theme	spectrometer	394:405	arg1	detection					416:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	3	46	theme	optimized	554:562	arg1	conditions					575:584	the optimized extraction conditions	550:584	the optimized extraction conditions	550:584	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	5	47	theme	regular	1058:1064	arg1	monitoring					1066:1075	regular monitoring	1058:1075	regular monitoring of trace heavy metal ions in real food samples	1058:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	48	theme	high	874:877	arg1	accuracy					879:886	high accuracy	874:886	high accuracy	874:886	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	1	49	theme	frameworks	301:310	arg1	composite					324:332	chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite	263:332	chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite	263:332	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	3	50	theme	0.008 µg	647:654	arg1	L-1					656:658	0.033 µg L-1 and 0.008 µg L-1	630:658	L-1	656:658	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	5	51	theme	ions	1098:1101	arg1	monitoring					1066:1075	regular monitoring	1058:1075	regular monitoring of trace heavy metal ions in real food samples	1058:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	3	52	theme	Pb2+	611:614	arg1	L-1					639:641	0.033 µg L-1 and 0.008 µg L-1	630:658	L-1	639:641	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	3	52	theme	Pb2+	611:614	arg1	limits					601:606	the detection limits	587:606	the detection limits of Pb2+ and Cd2+	587:623	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	5	53	from	Pb2+	912:915	arg1	wheat					974:978	wheat	974:978	wheat	974:978	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	53	from	Pb2+	912:915	arg1	rice					968:971	rice	968:971	rice	968:971	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	53	from	Pb2+	912:915	arg1	materials					957:965	various certified reference materials	929:965	various certified reference materials (rice, wheat and tea) with complicated matrices	929:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	53	from	Pb2+	912:915	arg1	tea					984:986	tea	984:986	tea	984:986	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	54	theme	food	1111:1114	arg1	samples					1116:1122	real food samples	1106:1122	real food samples	1106:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	1	55	theme	GF-AAS	408:413	arg1	detection					416:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	graphite furnace atomic absorption spectrometer (GF-AAS) detection	359:424	In this work, a facile solid phase extraction (SPE) method was developed for the analysis of trace Pb2+ and Cd2+ by using chitosan/thiol modified metal-organic frameworks (CS/MOF-SH) composite as adsorbent followed by graphite furnace atomic absorption spectrometer (GF-AAS) detection.					
32526650	5	56	theme	reference	947:955	arg1	wheat					974:978	wheat	974:978	wheat	974:978	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	56	theme	reference	947:955	arg1	rice					968:971	rice	968:971	rice	968:971	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	56	theme	reference	947:955	arg1	materials					957:965	various certified reference materials	929:965	various certified reference materials (rice, wheat and tea) with complicated matrices	929:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	56	theme	reference	947:955	arg1	tea					984:986	tea	984:986	tea	984:986	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	57	from	samples	1116:1122	arg1	monitoring					1066:1075	regular monitoring	1058:1075	regular monitoring of trace heavy metal ions in real food samples	1058:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	58	theme	certified	937:945	arg1	wheat					974:978	wheat	974:978	wheat	974:978	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	58	theme	certified	937:945	arg1	rice					968:971	rice	968:971	rice	968:971	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	58	theme	certified	937:945	arg1	materials					957:965	various certified reference materials	929:965	various certified reference materials (rice, wheat and tea) with complicated matrices	929:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	58	theme	certified	937:945	arg1	tea					984:986	tea	984:986	tea	984:986	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	0	59	from	ions	119:122	arg1	samples					132:138	food samples	127:138	food samples	127:138	Chitosan/thiol functionalized metal-organic framework composite for the simultaneous determination of lead and cadmium ions in food samples.					
32526650	4	60	theme	other	687:691	arg1	studies					693:699	other studies	687:699	other studies	687:699	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	5	61	theme	complicated	994:1004	arg1	matrices					1006:1013	complicated matrices	994:1013	complicated matrices	994:1013	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	4	62	contain	had	773:775	arg2	advantages					781:790	the advantages	777:790	the advantages of ease of handling and recyclability	777:828	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	4	62	contain	had	773:775	arg1	CS/MOF-SH					702:710	CS/MOF-SH	702:710	CS/MOF-SH	702:710	Compared to other studies, CS/MOF-SH not only possessed superior adsorption performance, but also had the advantages of ease of handling and recyclability.					
32526650	2	63	theme	adsorbent	483:491	arg1	dosage					493:498	adsorbent dosage	483:498	adsorbent dosage	483:498	The potential influencing factors, such as solution pH, adsorbent dosage, and extraction time, were fully estimated.					
32526650	5	64	from	monitoring	1066:1075	arg1	samples					1116:1122	real food samples	1106:1122	real food samples	1106:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	5	65	theme	developed	850:858	arg1	method					860:865	the developed method	846:865	the developed method	846:865	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
32526650	3	66	theme	detection	591:599	arg1	L-1					639:641	0.033 µg L-1 and 0.008 µg L-1	630:658	L-1	639:641	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	3	66	theme	detection	591:599	arg1	limits					601:606	the detection limits	587:606	the detection limits of Pb2+ and Cd2+	587:623	Under the optimized extraction conditions, the detection limits of Pb2+ and Cd2+ were 0.033 µg L-1 and 0.008 µg L-1, respectively.					
32526650	5	67	from	ions	1098:1101	arg1	samples					1116:1122	real food samples	1106:1122	real food samples	1106:1122	Encouragingly, the developed method was of high accuracy and could monitor trace Pb2+ and Cd2+ in various certified reference materials (rice, wheat and tea) with complicated matrices, demonstrating its practical potential for regular monitoring of trace heavy metal ions in real food samples.					
34015407	11	0	theme	DNA	1415:1417	arg1	fragmentation					1419:1431	significant DNA fragmentation	1403:1431	significant DNA fragmentation	1403:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	9	1	theme	normal	1190:1195	arg1	line					1210:1213	the normal retinal cell line	1186:1213	the normal retinal cell line	1186:1213	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	8	2	theme	Fourier-Transform	950:966	arg1	analysis					991:998	Fourier-Transform Infrared spectrometric analysis	950:998	Fourier-Transform Infrared spectrometric analysis	950:998	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	9	3	theme	cell	1205:1208	arg1	line					1210:1213	the normal retinal cell line	1186:1213	the normal retinal cell line	1186:1213	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	2	4	theme	levansucrase	280:291	arg1	production					293:302	levansucrase production	280:302	levansucrase production	280:302	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	1	5	theme	type	177:180	arg1	worldwide					182:190	the fourth most lethal cancer type worldwide	147:190	the fourth most lethal cancer type worldwide	147:190	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	1	5	theme	type	177:180	arg1	cancer					137:142	Pancreatic cancer	126:142	Pancreatic cancer	126:142	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	8	6	theme	spectrometric	977:989	arg1	analysis					991:998	Fourier-Transform Infrared spectrometric analysis	950:998	Fourier-Transform Infrared spectrometric analysis	950:998	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	7	7	theme	levan	815:819	arg1	yield					821:825	maximum levan yield	807:825	maximum levan yield (54.8 g/L)	807:836	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	7	7	theme	levan	815:819	arg1	g/L					833:835	54.8 g/L	828:835	54.8 g/L	828:835	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	4	8	theme	bacterial	440:448	arg1	isolates					456:463	eighteen bacterial honey isolates	431:463	eighteen bacterial honey isolates	431:463	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	11	9	theme	MCM7	1360:1363	arg1	genes					1365:1369	CXCR4 and MCM7 genes	1350:1369	CXCR4 and MCM7 genes	1350:1369	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	2	10	theme	anticancer	238:247	arg1	activities					249:258	anticancer activities	238:258	anticancer activities	238:258	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	0	11	from	evaluation	69:78	arg1	treatment					115:123	pancreatic cancer treatment	97:123	pancreatic cancer treatment	97:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	11	12	theme	CXCR4	1350:1354	arg1	genes					1365:1369	CXCR4 and MCM7 genes	1350:1369	CXCR4 and MCM7 genes	1350:1369	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	12	13	dep	Bacillus	1449:1456	arg1	subtilis					1458:1465	subtilis	1458:1465	subtilis	1458:1465	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	1	14	theme	Pancreatic	126:135	arg1	cancer					137:142	Pancreatic cancer	126:142	Pancreatic cancer	126:142	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	1	14	theme	Pancreatic	126:135	arg1	worldwide					182:190	the fourth most lethal cancer type worldwide	147:190	the fourth most lethal cancer type worldwide	147:190	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	10	15	theme	crude	1228:1232	arg1	levan					1234:1238	crude levan	1228:1238	crude levan	1228:1238	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	2	16	theme	related	269:275	arg1	studies					261:267	studies	261:267	studies related to levansucrase production	261:302	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	12	17	theme	chemoprotective	1563:1577	arg1	effects					1579:1585	both anti-cancer and chemoprotective effects	1542:1585	both anti-cancer and chemoprotective effects	1542:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	12	18	with	agents	1530:1535	arg1	effects					1579:1585	both anti-cancer and chemoprotective effects	1542:1585	both anti-cancer and chemoprotective effects	1542:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	1	19	theme	fourth	151:156	arg1	worldwide					182:190	the fourth most lethal cancer type worldwide	147:190	the fourth most lethal cancer type worldwide	147:190	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	1	19	theme	fourth	151:156	arg1	cancer					137:142	Pancreatic cancer	126:142	Pancreatic cancer	126:142	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	10	20	theme	mg/kg	1249:1253	arg1	weight					1260:1265	4833 mg/kg body weight	1244:1265	4833 mg/kg body weight	1244:1265	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	10	20	theme	mg/kg	1249:1253	arg1	LD50					1220:1223	The LD50	1216:1223	The LD50 of crude levan	1216:1238	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	0	21	theme	levan	83:87	arg1	role					89:92	levan role	83:92	levan role in pancreatic cancer treatment	83:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	5	22	theme	One-factor-at-a-time	574:593	arg1	technique					595:603	One-factor-at-a-time technique	574:603	One-factor-at-a-time technique	574:603	One-factor-at-a-time technique increased levansucrase activity by 60% when MgSO4 was eliminated.					
34015407	1	23	theme	lethal	163:168	arg1	worldwide					182:190	the fourth most lethal cancer type worldwide	147:190	the fourth most lethal cancer type worldwide	147:190	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	1	23	theme	lethal	163:168	arg1	cancer					137:142	Pancreatic cancer	126:142	Pancreatic cancer	126:142	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	3	24	theme	cancer	366:371	arg1	cells					373:377	pancreatic cancer cells	355:377	pancreatic cancer cells	355:377	To our knowledge, levan effect on pancreatic cancer cells has not been tested previously.					
34015407	4	25	theme	levan	512:516	arg1	g/L					528:530	33 g/L	525:530	33 g/L	525:530	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	4	25	theme	levan	512:516	arg1	yield					518:522	the highest levan yield	500:522	the highest levan yield (33 g/L)	500:531	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	9	26	theme	crude	1073:1077	arg1	levan					1092:1096	Both crude and dialyzed levan	1068:1096	Both crude and dialyzed levan	1068:1096	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	12	27	theme	anti-cancer	1547:1557	arg1	effects					1579:1585	both anti-cancer and chemoprotective effects	1542:1585	both anti-cancer and chemoprotective effects	1542:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	0	28	from	levansucrase	52:63	arg1	treatment					115:123	pancreatic cancer treatment	97:123	pancreatic cancer treatment	97:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	9	29	theme	dialyzed	1083:1090	arg1	levan					1092:1096	Both crude and dialyzed levan	1068:1096	Both crude and dialyzed levan	1068:1096	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	0	30	theme	cancer	108:113	arg1	treatment					115:123	pancreatic cancer treatment	97:123	pancreatic cancer treatment	97:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	4	31	dep	Bacillus	466:473	arg1	subtilis					475:482	subtilis	475:482	subtilis	475:482	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	11	32	theme	strong	1278:1283	arg1	activity					1297:1304	strong antioxidant activity	1278:1304	strong antioxidant activity	1278:1304	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	3	33	theme	levan	339:343	arg1	effect					345:350	levan effect	339:350	levan effect on pancreatic cancer cells	339:377	To our knowledge, levan effect on pancreatic cancer cells has not been tested previously.					
34015407	0	34	theme	Optimization	0:11	arg1	strategy					13:20	Optimization strategy	0:20	Optimization strategy of Bacillus subtilis	0:41	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	7	35	theme	media	785:789	arg1	composition					791:801	the media composition	781:801	the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL)	781:875	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	11	36	with	cells	1392:1396	arg1	fragmentation					1419:1431	significant DNA fragmentation	1403:1431	significant DNA fragmentation	1403:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	9	37	theme	cancer	1134:1139	arg1	line					1146:1149	the pancreatic cancer cell line	1119:1149	the pancreatic cancer cell line	1119:1149	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	0	38	theme	subtilis	34:41	arg1	strategy					13:20	Optimization strategy	0:20	Optimization strategy of Bacillus subtilis	0:41	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	7	39	theme	Burman	756:761	arg1	design					763:768	Placket Burman design	748:768	Placket Burman design	748:768	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	0	40	dep	levansucrase	52:63	arg1	strategy					13:20	Optimization strategy	0:20	Optimization strategy of Bacillus subtilis	0:41	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	8	41	theme	levan	900:904	arg1	identification					882:895	The identification	878:895	The identification of levan	878:904	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	2	42	theme	multiple	200:207	arg1	applications					215:226	multiple levan applications	200:226	multiple levan applications including anticancer activities	200:258	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	2	42	theme	multiple	200:207	arg1	activities					249:258	anticancer activities	238:258	anticancer activities	238:258	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	12	43	theme	promising	1487:1495	arg1	levan					1476:1480	Bacillus subtilis MT453867 levan	1449:1480	Bacillus subtilis MT453867 levan	1449:1480	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	12	43	theme	promising	1487:1495	arg1	adjunct					1497:1503	a promising adjunct	1485:1503	a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects	1485:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	11	44	theme	pancreatic	1374:1383	arg1	cells					1392:1396	pancreatic cancer cells	1374:1396	pancreatic cancer cells with significant DNA fragmentation	1374:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	6	45	theme	enzyme	720:725	arg1	activity					727:734	the enzyme activity	716:734	the enzyme activity (192 U/mL)	716:745	The addition of 60 g/L banana peels enhanced the enzyme activity (192 U/mL).					
34015407	6	45	theme	enzyme	720:725	arg1	U/mL					741:744	192 U/mL	737:744	192 U/mL	737:744	The addition of 60 g/L banana peels enhanced the enzyme activity (192 U/mL).					
34015407	12	46	theme	Bacillus	1449:1456	arg1	levan					1476:1480	Bacillus subtilis MT453867 levan	1449:1480	Bacillus subtilis MT453867 levan	1449:1480	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	12	46	theme	Bacillus	1449:1456	arg1	adjunct					1497:1503	a promising adjunct	1485:1503	a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects	1485:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	8	47	theme	thin-layer	923:932	arg1	chromatography					934:947	thin-layer chromatography	923:947	thin-layer chromatography	923:947	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	11	48	theme	genes	1365:1369	arg1	expression					1336:1345	the expression	1332:1345	the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation	1332:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	12	49	theme	MT453867	1467:1474	arg1	levan					1476:1480	Bacillus subtilis MT453867 levan	1449:1480	Bacillus subtilis MT453867 levan	1449:1480	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	12	49	theme	MT453867	1467:1474	arg1	adjunct					1497:1503	a promising adjunct	1485:1503	a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects	1485:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	11	50	theme	significant	1403:1413	arg1	fragmentation					1419:1431	significant DNA fragmentation	1403:1431	significant DNA fragmentation	1403:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	7	51	theme	levansucrase	842:853	arg1	production					855:864	levansucrase production	842:864	levansucrase production (505 U/mL)	842:875	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	7	51	theme	levansucrase	842:853	arg1	U/mL					871:874	505 U/mL	867:874	505 U/mL	867:874	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	8	52	theme	Infrared	968:975	arg1	analysis					991:998	Fourier-Transform Infrared spectrometric analysis	950:998	Fourier-Transform Infrared spectrometric analysis	950:998	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	9	53	theme	retinal	1197:1203	arg1	line					1210:1213	the normal retinal cell line	1186:1213	the normal retinal cell line	1186:1213	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	1	54	theme	cancer	170:175	arg1	worldwide					182:190	the fourth most lethal cancer type worldwide	147:190	the fourth most lethal cancer type worldwide	147:190	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	1	54	theme	cancer	170:175	arg1	cancer					137:142	Pancreatic cancer	126:142	Pancreatic cancer	126:142	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	9	55	from	cytotoxicity	1170:1181	arg1	line					1210:1213	the normal retinal cell line	1186:1213	the normal retinal cell line	1186:1213	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	8	56	theme	13C-nuclear-magnetic	1001:1020	arg1	resonance					1022:1030	13C-nuclear-magnetic resonance	1001:1030	13C-nuclear-magnetic resonance	1001:1030	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	12	57	from	adjunct	1497:1503	arg1	conclusion					1437:1446	conclusion	1437:1446	conclusion	1437:1446	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	4	58	theme	honey	450:454	arg1	isolates					456:463	eighteen bacterial honey isolates	431:463	eighteen bacterial honey isolates	431:463	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	7	59	theme	maximum	807:813	arg1	yield					821:825	maximum levan yield	807:825	maximum levan yield (54.8 g/L)	807:836	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	7	59	theme	maximum	807:813	arg1	g/L					833:835	54.8 g/L	828:835	54.8 g/L	828:835	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	0	60	from	treatment	115:123	arg1	evaluation					69:78	evaluation	69:78	evaluation of levan role in pancreatic cancer treatment	69:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	0	60	from	treatment	115:123	arg1	levansucrase					52:63	MT453867 levansucrase	43:63	MT453867 levansucrase	43:63	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	10	61	theme	levan	1234:1238	arg1	LD50					1220:1223	The LD50	1216:1223	The LD50 of crude levan	1216:1238	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	10	61	theme	levan	1234:1238	arg1	weight					1260:1265	4833 mg/kg body weight	1244:1265	4833 mg/kg body weight	1244:1265	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	3	62	from	effect	345:350	arg1	cells					373:377	pancreatic cancer cells	355:377	pancreatic cancer cells	355:377	To our knowledge, levan effect on pancreatic cancer cells has not been tested previously.					
34015407	4	63	theme	highest	504:510	arg1	g/L					528:530	33 g/L	525:530	33 g/L	525:530	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	4	63	theme	highest	504:510	arg1	yield					518:522	the highest levan yield	500:522	the highest levan yield (33 g/L)	500:531	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	10	64	theme	body	1255:1258	arg1	weight					1260:1265	4833 mg/kg body weight	1244:1265	4833 mg/kg body weight	1244:1265	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	10	64	theme	body	1255:1258	arg1	LD50					1220:1223	The LD50	1216:1223	The LD50 of crude levan	1216:1238	The LD50 of crude levan was 4833 mg/kg body weight.					
34015407	0	65	theme	role	89:92	arg1	evaluation					69:78	evaluation	69:78	evaluation of levan role in pancreatic cancer treatment	69:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	0	65	theme	role	89:92	arg1	levansucrase					52:63	MT453867 levansucrase	43:63	MT453867 levansucrase	43:63	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	1	66	theme	most	158:161	arg1	worldwide					182:190	the fourth most lethal cancer type worldwide	147:190	the fourth most lethal cancer type worldwide	147:190	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	1	66	theme	most	158:161	arg1	cancer					137:142	Pancreatic cancer	126:142	Pancreatic cancer	126:142	Pancreatic cancer is the fourth most lethal cancer type worldwide.					
34015407	0	67	from	role	89:92	arg1	treatment					115:123	pancreatic cancer treatment	97:123	pancreatic cancer treatment	97:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	0	68	theme	pancreatic	97:106	arg1	treatment					115:123	pancreatic cancer treatment	97:123	pancreatic cancer treatment	97:123	Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.					
34015407	8	69	theme	1H-nuclear-magnetic	1037:1055	arg1	resonance					1057:1065	1H-nuclear-magnetic resonance	1037:1065	1H-nuclear-magnetic resonance	1037:1065	The identification of levan was confirmed by thin-layer chromatography, Fourier-Transform Infrared spectrometric analysis, 13C-nuclear-magnetic resonance, and 1H-nuclear-magnetic resonance.					
34015407	6	70	theme	peels	701:705	arg1	addition					675:682	The addition	671:682	The addition of 60 g/L banana peels	671:705	The addition of 60 g/L banana peels enhanced the enzyme activity (192 U/mL).					
34015407	5	71	theme	levansucrase	615:626	arg1	activity					628:635	levansucrase activity	615:635	levansucrase activity	615:635	One-factor-at-a-time technique increased levansucrase activity by 60% when MgSO4 was eliminated.					
34015407	3	72	theme	pancreatic	355:364	arg1	cells					373:377	pancreatic cancer cells	355:377	pancreatic cancer cells	355:377	To our knowledge, levan effect on pancreatic cancer cells has not been tested previously.					
34015407	6	73	theme	g/L	690:692	arg1	peels					701:705	60 g/L banana peels	687:705	60 g/L banana peels	687:705	The addition of 60 g/L banana peels enhanced the enzyme activity (192 U/mL).					
34015407	11	74	from	expression	1336:1345	arg1	cells					1392:1396	pancreatic cancer cells	1374:1396	pancreatic cancer cells with significant DNA fragmentation	1374:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	4	75	theme	Bacillus	466:473	arg1	MT453867					484:491	Bacillus subtilis MT453867	466:491	Bacillus subtilis MT453867	466:491	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	4	76	theme	levansucrase	537:548	arg1	production					550:559	levansucrase production	537:559	levansucrase production (8.31 U/mL)	537:571	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	4	76	theme	levansucrase	537:548	arg1	U/mL					567:570	8.31 U/mL	562:570	8.31 U/mL	562:570	In this work, among eighteen bacterial honey isolates, Bacillus subtilis MT453867 showed the highest levan yield (33 g/L) and levansucrase production (8.31 U/mL).					
34015407	11	77	theme	antioxidant	1285:1295	arg1	activity					1297:1304	strong antioxidant activity	1278:1304	strong antioxidant activity	1278:1304	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	9	78	theme	pancreatic	1123:1132	arg1	line					1146:1149	the pancreatic cancer cell line	1119:1149	the pancreatic cancer cell line	1119:1149	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	6	79	theme	banana	694:699	arg1	peels					701:705	60 g/L banana peels	687:705	60 g/L banana peels	687:705	The addition of 60 g/L banana peels enhanced the enzyme activity (192 U/mL).					
34015407	11	80	contain	had	1274:1276	arg1	Levan					1268:1272	Levan	1268:1272	Levan	1268:1272	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	11	80	contain	had	1274:1276	arg2	activity					1297:1304	strong antioxidant activity	1278:1304	strong antioxidant activity	1278:1304	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34015407	9	81	theme	cell	1141:1144	arg1	line					1146:1149	the pancreatic cancer cell line	1119:1149	the pancreatic cancer cell line	1119:1149	Both crude and dialyzed levan completely inhibited the pancreatic cancer cell line at 100 ppm with no cytotoxicity on the normal retinal cell line.					
34015407	12	82	theme	pancreatic-anticancer	1508:1528	arg1	agents					1530:1535	pancreatic-anticancer agents	1508:1535	pancreatic-anticancer agents with both anti-cancer and chemoprotective effects	1508:1585	In conclusion, Bacillus subtilis MT453867 levan is a promising adjunct to pancreatic-anticancer agents with both anti-cancer and chemoprotective effects.					
34015407	2	83	theme	levan	209:213	arg1	applications					215:226	multiple levan applications	200:226	multiple levan applications including anticancer activities	200:258	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	2	83	theme	levan	209:213	arg1	activities					249:258	anticancer activities	238:258	anticancer activities	238:258	Due to multiple levan applications including anticancer activities, studies related to levansucrase production are of interest.					
34015407	7	84	theme	Placket	748:754	arg1	design					763:768	Placket Burman design	748:768	Placket Burman design	748:768	Placket Burman design determined the media composition for maximum levan yield (54.8 g/L) and levansucrase production (505 U/mL).					
34015407	11	85	theme	cancer	1385:1390	arg1	cells					1392:1396	pancreatic cancer cells	1374:1396	pancreatic cancer cells with significant DNA fragmentation	1374:1431	Levan had strong antioxidant activity and significantly reduced the expression of CXCR4 and MCM7 genes in pancreatic cancer cells with significant DNA fragmentation.					
34119546	0	0	theme	hypoglycemic	81:92	arg1	agent					94:98	A potential hypoglycemic agent	69:98	A potential hypoglycemic agent	69:98	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	4	1	theme	bovine	816:821	arg1	CS					823:824	bovine CS	816:824	bovine CS	816:824	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	4	2	dep	analyses	726:733	arg1	the					687:689	the	687:689	the	687:689	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	4	2	dep	analyses	726:733	arg1	data					691:694	data	691:694	data	691:694	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	6	3	from	shark	1196:1200	arg1	ingredient					1279:1288	a novel functional food ingredient	1255:1288	a novel functional food ingredient in T2DM management	1255:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	3	from	shark	1196:1200	arg1	CSs					1187:1189	the CSs	1183:1189	the CSs from shark and pig as α-amylase inhibitors	1183:1232	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	2	4	theme	α-amylase	451:459	arg1	effects					428:434	the effects	424:434	the effects on activity of α-amylase and blood glucose	424:477	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	0	5	from	effects	11:17	arg1	activity					59:66	alpha-amylase activity	45:66	alpha-amylase activity	45:66	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	1	6	theme	essential	255:263	arg1	way					265:267	an essential way	252:267	an essential way for the treatment of Type-II diabetes mellitus (T2DM)	252:321	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	3	7	theme	bovine	654:659	arg1	CS					661:662	the bovine CS	650:662	the bovine CS	650:662	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	6	8	theme	α-amylase	1213:1221	arg1	inhibitors					1223:1232	α-amylase inhibitors	1213:1232	α-amylase inhibitors	1213:1232	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	9	theme	subunit	1120:1126	arg1	composition					1128:1138	disaccharide subunit composition	1107:1138	disaccharide subunit composition	1107:1138	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	9	theme	subunit	1120:1126	arg1	same					1155:1158	same	1155:1158	same	1155:1158	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	9	theme	subunit	1120:1126	arg1	weight					1096:1101	molecular weight	1086:1101	molecular weight	1086:1101	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	5	10	theme	CSs	897:899	arg1	administration					861:874	oral administration	856:874	oral administration of shark and porcine CSs	856:899	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	6	11	theme	molecular	1086:1094	arg1	composition					1128:1138	disaccharide subunit composition	1107:1138	disaccharide subunit composition	1107:1138	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	11	theme	molecular	1086:1094	arg1	weight					1096:1101	molecular weight	1086:1101	molecular weight	1086:1101	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	11	theme	molecular	1086:1094	arg1	same					1155:1158	same	1155:1158	same	1155:1158	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	12	from	pig	1206:1208	arg1	ingredient					1279:1288	a novel functional food ingredient	1255:1288	a novel functional food ingredient in T2DM management	1255:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	12	from	pig	1206:1208	arg1	CSs					1187:1189	the CSs	1183:1189	the CSs from shark and pig as α-amylase inhibitors	1183:1232	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	13	theme	disaccharide	1107:1118	arg1	composition					1128:1138	disaccharide subunit composition	1107:1138	disaccharide subunit composition	1107:1138	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	13	theme	disaccharide	1107:1118	arg1	same					1155:1158	same	1155:1158	same	1155:1158	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	13	theme	disaccharide	1107:1118	arg1	weight					1096:1101	molecular weight	1086:1101	molecular weight	1086:1101	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	4	14	theme	spectroscopic	712:724	arg1	analyses					726:733	fluorescence spectroscopic analyses	699:733	fluorescence spectroscopic analyses	699:733	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	6	15	theme	T2DM	1293:1296	arg1	management					1298:1307	T2DM management	1293:1307	T2DM management	1293:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	2	16	from	effects	428:434	arg1	activity					439:446	activity	439:446	activity	439:446	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	6	17	theme	different	1017:1025	arg1	sources					1027:1033	different sources	1017:1033	different sources	1017:1033	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	2	18	theme	sulfate	384:390	arg1	information					357:367	the structural information	342:367	the structural information of chondroitin sulfate (CS) from different origins	342:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	0	19	dep	agent	94:98	arg1	effects					11:17	Inhibitory effects	0:17	Inhibitory effects of chondroitin sulfate on alpha-amylase activity	0:66	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	2	20	theme	chondroitin	372:382	arg1	CS					393:394	CS	393:394	CS	393:394	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	2	20	theme	chondroitin	372:382	arg1	sulfate					384:390	chondroitin sulfate	372:390	chondroitin sulfate (CS) from different origins	372:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	0	21	theme	potential	71:79	arg1	agent					94:98	A potential hypoglycemic agent	69:98	A potential hypoglycemic agent	69:98	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	3	22	with	obvious	581:587	arg1	values					599:604	IC50 values	594:604	IC50 values	594:604	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	3	23	theme	porcine	539:545	arg1	CSs					547:549	porcine CSs	539:549	porcine CSs	539:549	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	0	24	theme	Inhibitory	0:9	arg1	effects					11:17	Inhibitory effects	0:17	Inhibitory effects of chondroitin sulfate on alpha-amylase activity	0:66	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	5	25	theme	postprandial	924:935	arg1	levels					951:956	postprandial blood glucose levels	924:956	postprandial blood glucose levels	924:956	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	4	26	theme	enzyme	800:805	arg1	intensity					783:791	fluorescence intensity	770:791	fluorescence intensity of the enzyme	770:805	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	3	27	theme	CSs	547:549	arg1	effects					518:524	The inhibitory effects	503:524	The inhibitory effects of shark and porcine CSs against α-amylase activity	503:576	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	3	27	theme	CSs	547:549	arg1	obvious					581:587	obvious	581:587	obvious	581:587	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	6	28	theme	food	1274:1277	arg1	CSs					1187:1189	the CSs	1183:1189	the CSs from shark and pig as α-amylase inhibitors	1183:1232	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	28	theme	food	1274:1277	arg1	ingredient					1279:1288	a novel functional food ingredient	1255:1288	a novel functional food ingredient in T2DM management	1255:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	1	29	theme	starch	194:199	arg1	degradation					179:189	the degradation	175:189	the degradation of starch	175:199	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	0	30	theme	chondroitin	22:32	arg1	sulfate					34:40	chondroitin sulfate	22:40	chondroitin sulfate	22:40	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	4	31	theme	fluorescence	770:781	arg1	intensity					783:791	fluorescence intensity	770:791	fluorescence intensity of the enzyme	770:805	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	3	32	theme	shark	529:533	arg1	effects					518:524	The inhibitory effects	503:524	The inhibitory effects of shark and porcine CSs against α-amylase activity	503:576	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	3	32	theme	shark	529:533	arg1	obvious					581:587	obvious	581:587	obvious	581:587	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	2	33	theme	different	402:410	arg1	origins					412:418	different origins	402:418	different origins	402:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	6	34	from	ingredient	1279:1288	arg1	management					1298:1307	T2DM management	1293:1307	T2DM management	1293:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	5	35	theme	shark	879:883	arg1	CSs					897:899	shark and porcine CSs	879:899	CSs	897:899	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	5	36	theme	porcine	889:895	arg1	CSs					897:899	shark and porcine CSs	879:899	CSs	897:899	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	2	37	theme	structural	346:355	arg1	information					357:367	the structural information	342:367	the structural information of chondroitin sulfate (CS) from different origins	342:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	6	38	theme	biological	1052:1061	arg1	functions					1063:1071	different biological functions	1042:1071	different biological functions	1042:1071	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	1	39	theme	Type-II	290:296	arg1	mellitus					307:314	Type-II diabetes mellitus	290:314	Type-II diabetes mellitus (T2DM)	290:321	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	1	39	theme	Type-II	290:296	arg1	T2DM					317:320	T2DM	317:320	T2DM	317:320	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	6	40	theme	functional	1263:1272	arg1	CSs					1187:1189	the CSs	1183:1189	the CSs from shark and pig as α-amylase inhibitors	1183:1232	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	40	theme	functional	1263:1272	arg1	ingredient					1279:1288	a novel functional food ingredient	1255:1288	a novel functional food ingredient in T2DM management	1255:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	3	41	theme	14.42 mg/ml	619:629	arg1	values					599:604	IC50 values	594:604	IC50 values	594:604	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	5	42	theme	normal	961:966	arg1	mice					981:984	normal and diabetic mice	961:984	normal and diabetic mice	961:984	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	0	43	theme	sulfate	34:40	arg1	effects					11:17	Inhibitory effects	0:17	Inhibitory effects of chondroitin sulfate on alpha-amylase activity	0:66	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	1	44	theme	diabetes	298:305	arg1	mellitus					307:314	Type-II diabetes mellitus	290:314	Type-II diabetes mellitus (T2DM)	290:321	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	1	44	theme	diabetes	298:305	arg1	T2DM					317:320	T2DM	317:320	T2DM	317:320	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	6	45	theme	novel	1257:1261	arg1	CSs					1187:1189	the CSs	1183:1189	the CSs from shark and pig as α-amylase inhibitors	1183:1232	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	6	45	theme	novel	1257:1261	arg1	ingredient					1279:1288	a novel functional food ingredient	1255:1288	a novel functional food ingredient in T2DM management	1255:1307	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	1	46	theme	mellitus	307:314	arg1	treatment					277:285	the treatment	273:285	the treatment of Type-II diabetes mellitus (T2DM)	273:321	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	3	47	theme	11.97	609:613	arg1	values					599:604	IC50 values	594:604	IC50 values	594:604	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	5	48	theme	diabetic	972:979	arg1	mice					981:984	normal and diabetic mice	961:984	normal and diabetic mice	961:984	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	2	49	from	information	357:367	arg1	origins					412:418	different origins	402:418	different origins	402:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	0	50	theme	alpha-amylase	45:57	arg1	activity					59:66	alpha-amylase activity	45:66	alpha-amylase activity	45:66	Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent.					
34119546	3	51	theme	α-amylase	559:567	arg1	activity					569:576	α-amylase activity	559:576	α-amylase activity	559:576	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	5	52	theme	blood	937:941	arg1	levels					951:956	postprandial blood glucose levels	924:956	postprandial blood glucose levels	924:956	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	3	53	theme	IC50	594:597	arg1	values					599:604	IC50 values	594:604	IC50 values	594:604	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	4	54	from	quench	759:764	arg1	CSs					736:738	CSs	736:738	CSs from shark and pig quench	736:764	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	5	55	theme	oral	856:859	arg1	administration					861:874	oral administration	856:874	oral administration of shark and porcine CSs	856:899	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	4	56	theme	fluorescence	699:710	arg1	analyses					726:733	fluorescence spectroscopic analyses	699:733	fluorescence spectroscopic analyses	699:733	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	1	57	theme	blood	226:230	arg1	glucose					232:238	blood glucose	226:238	blood glucose	226:238	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	3	58	theme	inhibitory	507:516	arg1	effects					518:524	The inhibitory effects	503:524	The inhibitory effects of shark and porcine CSs against α-amylase activity	503:576	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	3	58	theme	inhibitory	507:516	arg1	obvious					581:587	obvious	581:587	obvious	581:587	The inhibitory effects of shark and porcine CSs against α-amylase activity is obvious with IC50 values of 11.97 and 14.42 mg/ml, respectively, but the bovine CS almost no effect.					
34119546	5	59	theme	glucose	943:949	arg1	levels					951:956	postprandial blood glucose levels	924:956	postprandial blood glucose levels	924:956	In vivo, oral administration of shark and porcine CSs efficiently suppresses postprandial blood glucose levels in normal and diabetic mice.					
34119546	1	60	theme	intestinal	132:141	arg1	α-amylase					150:158	the intestinal enzyme α-amylase	128:158	the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose	128:212	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	2	61	theme	glucose	471:477	arg1	effects					428:434	the effects	424:434	the effects on activity of α-amylase and blood glucose	424:477	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	6	62	theme	different	1042:1050	arg1	functions					1063:1071	different biological functions	1042:1071	different biological functions	1042:1071	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	4	63	theme	pig	755:757	arg1	quench					759:764	pig quench	755:764	pig quench	755:764	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	6	64	from	sources	1027:1033	arg1	CSs					1008:1010	CSs	1008:1010	CSs from different sources	1008:1033	Our study found that CSs from different sources showed different biological functions even if both molecular weight and disaccharide subunit composition are almost the same, and demonstrated that the CSs from shark and pig as α-amylase inhibitors could be regarded as a novel functional food ingredient in T2DM management.					
34119546	1	65	theme	enzyme	143:148	arg1	α-amylase					150:158	the intestinal enzyme α-amylase	128:158	the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose	128:212	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
34119546	2	66	theme	blood	465:469	arg1	glucose					471:477	blood glucose	465:477	blood glucose	465:477	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	2	67	from	origins	412:418	arg1	CS					393:394	CS	393:394	CS	393:394	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	2	67	from	origins	412:418	arg1	information					357:367	the structural information	342:367	the structural information of chondroitin sulfate (CS) from different origins	342:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	2	67	from	origins	412:418	arg1	sulfate					384:390	chondroitin sulfate	372:390	chondroitin sulfate (CS) from different origins	372:418	Here, we compared the structural information of chondroitin sulfate (CS) from different origins and the effects on activity of α-amylase and blood glucose have been investigated.					
34119546	4	68	from	shark	745:749	arg1	CSs					736:738	CSs	736:738	CSs from shark and pig quench	736:764	From the data of fluorescence spectroscopic analyses, CSs from shark and pig quench Try fluorescence intensity of the enzyme, whereas bovine CS induces an increase.					
34119546	1	69	theme	α-amylase	150:158	arg1	activity					116:123	the activity	112:123	the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose	112:212	Inhibiting the activity of the intestinal enzyme α-amylase that catalyzes the degradation of starch into glucose can control blood glucose and provide an essential way for the treatment of Type-II diabetes mellitus (T2DM).					
33169606	8	0	theme	Fenton	1303:1308	arg1	catalysts					1310:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts	1251:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials	1251:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	4	1	from	2-10	846:849	arg1	presence					858:865	the presence	854:865	the presence of H2O2	854:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	1	2	theme	advanced	187:194	arg1	catalysts					208:216	biocompatible advanced Fenton-like catalysts	173:216	biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials	173:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	1	3	theme	sustainable	288:298	arg1	materials					311:319	sustainable biosourced materials	288:319	sustainable biosourced materials	288:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	2	4	theme	Fenton-like	530:540	arg1	catalysts					542:550	advanced Fenton-like catalysts	521:550	advanced Fenton-like catalysts	521:550	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	8	5	theme	fabricating	1251:1261	arg1	catalysts					1310:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts	1251:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials	1251:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	1	6	theme	Fenton-like	196:206	arg1	catalysts					208:216	biocompatible advanced Fenton-like catalysts	173:216	biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials	173:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	8	7	theme	biocompatible	1263:1275	arg1	catalysts					1310:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts	1251:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials	1251:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	1	8	theme	biosourced	300:309	arg1	materials					311:319	sustainable biosourced materials	288:319	sustainable biosourced materials	288:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	0	9	theme	Organic	124:130	arg1	Dyes					132:135	Organic Dyes	124:135	Organic Dyes	124:135	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	4	10	from	range	837:841	arg1	presence					858:865	the presence	854:865	the presence of H2O2	854:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	4	11	theme	pH	834:835	arg1	range					837:841	a wide pH range	827:841	a wide pH range of 2-10 in the presence of H2O2	827:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	3	12	theme	MCNF/PDA	609:616	arg1	nanocomposites					618:631	The MCNF/PDA nanocomposites	605:631	The MCNF/PDA nanocomposites	605:631	The MCNF/PDA nanocomposites were prepared by anchoring Fe3O4 nanoparticles to CNFs via chemical deposition followed with PDA coatings.					
33169606	2	13	theme	novel	395:399	arg1	MCNF/PDA					451:458	MCNF/PDA	451:458	MCNF/PDA	451:458	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	13	theme	novel	395:399	arg1	nanocomposite					436:448	a novel mussel-inspired magnetic cellulose nanocomposite	393:448	a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF)	393:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	4	14	theme	blue	814:817	arg1	methylene					804:812	methylene blue	804:817	methylene blue (MB)	804:822	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	4	14	theme	blue	814:817	arg1	MB					820:821	MB	820:821	MB	820:821	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	2	15	theme	organic	575:581	arg1	dyes					583:586	organic dyes	575:586	organic dyes	575:586	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	4	16	theme	maximum	886:892	arg1	capacity					906:913	a maximum degradation capacity	884:913	a maximum degradation capacity of 2265 mg/g	884:926	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	6	17	theme	high	1075:1078	arg1	rate					1067:1070	the removal rate	1055:1070	the removal rate as high as 95%	1055:1085	After four cycles, it can still achieve the removal rate as high as 95%.					
33169606	1	18	theme	catalysts	208:216	arg1	development					158:168	The development	154:168	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials	154:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	7	19	theme	degradation	1158:1168	arg1	performance					1170:1180	an excellent degradation performance	1145:1180	an excellent degradation performance to the antibiotic tetracycline	1145:1211	In addition, the MCNF/PDA nanocatalysts also demonstrate an excellent degradation performance to the antibiotic tetracycline.					
33169606	5	20	theme	MCNF/PDA	943:950	arg1	nanocatalysts					952:964	the MCNF/PDA nanocatalysts	939:964	the MCNF/PDA nanocatalysts	939:964	Moreover, the MCNF/PDA nanocatalysts are highly stable and can be easily regenerated.					
33169606	5	20	theme	MCNF/PDA	943:950	arg1	stable					977:982	stable	977:982	stable	977:982	Moreover, the MCNF/PDA nanocatalysts are highly stable and can be easily regenerated.					
33169606	7	21	theme	MCNF/PDA	1105:1112	arg1	nanocatalysts					1114:1126	the MCNF/PDA nanocatalysts	1101:1126	the MCNF/PDA nanocatalysts	1101:1126	In addition, the MCNF/PDA nanocatalysts also demonstrate an excellent degradation performance to the antibiotic tetracycline.					
33169606	7	22	theme	excellent	1148:1156	arg1	performance					1170:1180	an excellent degradation performance	1145:1180	an excellent degradation performance to the antibiotic tetracycline	1145:1211	In addition, the MCNF/PDA nanocatalysts also demonstrate an excellent degradation performance to the antibiotic tetracycline.					
33169606	8	23	theme	cellulosic-based	1277:1292	arg1	catalysts					1310:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts	1251:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials	1251:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	0	24	theme	Advanced	12:19	arg1	Catalysts					33:41	Sustainable Advanced Fenton-like Catalysts	0:41	Sustainable Advanced Fenton-like Catalysts	0:41	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	8	25	from	wastewater	1409:1418	arg1	pollutants					1393:1402	organic pollutants	1385:1402	organic pollutants from wastewater	1385:1418	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	1	26	theme	high	223:226	arg1	activity					238:245	high catalytic activity	223:245	high catalytic activity	223:245	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	0	27	theme	Sustainable	0:10	arg1	Catalysts					33:41	Sustainable Advanced Fenton-like Catalysts	0:41	Sustainable Advanced Fenton-like Catalysts	0:41	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	4	28	theme	degradation	894:904	arg1	capacity					906:913	a maximum degradation capacity	884:913	a maximum degradation capacity of 2265 mg/g	884:926	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	1	29	theme	catalytic	228:236	arg1	activity					238:245	high catalytic activity	223:245	high catalytic activity	223:245	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	8	30	theme	new	1233:1235	arg1	insights					1237:1244	new insights	1233:1244	new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials	1233:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	1	31	theme	considerable	327:338	arg1	interest					340:347	considerable interest	327:347	considerable interest	327:347	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	2	32	theme	cellulose	479:487	arg1	CNF					501:503	CNF	501:503	CNF	501:503	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	32	theme	cellulose	479:487	arg1	nanofibers					489:498	carboxylated cellulose nanofibers	466:498	carboxylated cellulose nanofibers (CNF)	466:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	0	33	theme	Fenton-like	21:31	arg1	Catalysts					33:41	Sustainable Advanced Fenton-like Catalysts	0:41	Sustainable Advanced Fenton-like Catalysts	0:41	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	3	34	theme	PDA	726:728	arg1	coatings					730:737	PDA coatings	726:737	PDA coatings	726:737	The MCNF/PDA nanocomposites were prepared by anchoring Fe3O4 nanoparticles to CNFs via chemical deposition followed with PDA coatings.					
33169606	4	35	theme	degradation	776:786	arg1	activity					788:795	an excellent degradation activity	763:795	an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2	763:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	2	36	theme	carboxylated	466:477	arg1	CNF					501:503	CNF	501:503	CNF	501:503	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	36	theme	carboxylated	466:477	arg1	nanofibers					489:498	carboxylated cellulose nanofibers	466:498	carboxylated cellulose nanofibers (CNF)	466:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	37	theme	advanced	521:528	arg1	catalysts					542:550	advanced Fenton-like catalysts	521:550	advanced Fenton-like catalysts	521:550	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	4	38	theme	H2O2	870:873	arg1	presence					858:865	the presence	854:865	the presence of H2O2	854:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	2	39	theme	cellulose	426:434	arg1	MCNF/PDA					451:458	MCNF/PDA	451:458	MCNF/PDA	451:458	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	39	theme	cellulose	426:434	arg1	nanocomposite					436:448	a novel mussel-inspired magnetic cellulose nanocomposite	393:448	a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF)	393:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	1	40	theme	good	248:251	arg1	stability					253:261	good stability	248:261	good stability	248:261	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	4	41	theme	excellent	766:774	arg1	activity					788:795	an excellent degradation activity	763:795	an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2	763:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	4	42	contain	have	879:882	arg2	capacity					906:913	a maximum degradation capacity	884:913	a maximum degradation capacity of 2265 mg/g	884:926	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	4	42	contain	have	879:882	arg1	composites					744:753	The composites	740:753	The composites	740:753	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	4	43	theme	wide	829:832	arg1	range					837:841	a wide pH range	827:841	a wide pH range of 2-10 in the presence of H2O2	827:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	8	44	theme	biomass-derived	1337:1351	arg1	materials					1353:1361	sustainable biomass-derived materials	1325:1361	sustainable biomass-derived materials	1325:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	0	45	theme	Magnetic	68:75	arg1	Nanocomposites					87:100	Mussel-Inspired Magnetic Cellulose Nanocomposites	52:100	Mussel-Inspired Magnetic Cellulose Nanocomposites	52:100	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	0	46	theme	Mussel-Inspired	52:66	arg1	Nanocomposites					87:100	Mussel-Inspired Magnetic Cellulose Nanocomposites	52:100	Mussel-Inspired Magnetic Cellulose Nanocomposites	52:100	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	8	47	with	catalysts	1310:1318	arg1	materials					1353:1361	sustainable biomass-derived materials	1325:1361	sustainable biomass-derived materials	1325:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	3	48	theme	Fe3O4	660:664	arg1	nanoparticles					666:678	Fe3O4 nanoparticles	660:678	Fe3O4 nanoparticles to CNFs	660:686	The MCNF/PDA nanocomposites were prepared by anchoring Fe3O4 nanoparticles to CNFs via chemical deposition followed with PDA coatings.					
33169606	1	49	with	catalysts	208:216	arg1	recyclability					268:280	recyclability	268:280	recyclability using sustainable biosourced materials	268:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	1	49	with	catalysts	208:216	arg1	activity					238:245	high catalytic activity	223:245	high catalytic activity	223:245	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	1	49	with	catalysts	208:216	arg1	stability					253:261	good stability	248:261	good stability	248:261	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	8	50	link	biomass-derived	1337:1351	arg1	materials					1353:1361	sustainable biomass-derived materials	1325:1361	sustainable biomass-derived materials	1325:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	3	51	theme	chemical	692:699	arg1	deposition					701:710	chemical deposition	692:710	chemical deposition followed with PDA coatings	692:737	The MCNF/PDA nanocomposites were prepared by anchoring Fe3O4 nanoparticles to CNFs via chemical deposition followed with PDA coatings.					
33169606	8	52	theme	organic	1385:1391	arg1	pollutants					1393:1402	organic pollutants	1385:1402	organic pollutants from wastewater	1385:1418	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	6	53	theme	removal	1059:1065	arg1	rate					1067:1070	the removal rate	1055:1070	the removal rate as high as 95%	1055:1085	After four cycles, it can still achieve the removal rate as high as 95%.					
33169606	4	54	theme	2-10	846:849	arg1	range					837:841	a wide pH range	827:841	a wide pH range of 2-10 in the presence of H2O2	827:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	4	55	from	presence	858:865	arg1	range					837:841	a wide pH range	827:841	a wide pH range of 2-10 in the presence of H2O2	827:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	1	56	theme	biocompatible	173:185	arg1	catalysts					208:216	biocompatible advanced Fenton-like catalysts	173:216	biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials	173:319	The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge.					
33169606	4	57	theme	mg/g	923:926	arg1	capacity					906:913	a maximum degradation capacity	884:913	a maximum degradation capacity of 2265 mg/g	884:926	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
33169606	8	58	theme	sustainable	1325:1335	arg1	materials					1353:1361	sustainable biomass-derived materials	1325:1361	sustainable biomass-derived materials	1325:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	2	59	theme	magnetic	417:424	arg1	MCNF/PDA					451:458	MCNF/PDA	451:458	MCNF/PDA	451:458	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	59	theme	magnetic	417:424	arg1	nanocomposite					436:448	a novel mussel-inspired magnetic cellulose nanocomposite	393:448	a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF)	393:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	0	60	theme	Cellulose	77:85	arg1	Nanocomposites					87:100	Mussel-Inspired Magnetic Cellulose Nanocomposites	52:100	Mussel-Inspired Magnetic Cellulose Nanocomposites	52:100	Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.					
33169606	2	61	with	nanocomposite	436:448	arg1	CNF					501:503	CNF	501:503	CNF	501:503	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	61	with	nanocomposite	436:448	arg1	nanofibers					489:498	carboxylated cellulose nanofibers	466:498	carboxylated cellulose nanofibers (CNF)	466:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	7	62	theme	antibiotic	1189:1198	arg1	tetracycline					1200:1211	the antibiotic tetracycline	1185:1211	the antibiotic tetracycline	1185:1211	In addition, the MCNF/PDA nanocatalysts also demonstrate an excellent degradation performance to the antibiotic tetracycline.					
33169606	8	63	theme	advanced	1294:1301	arg1	catalysts					1310:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts	1251:1318	fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials	1251:1361	This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.					
33169606	2	64	theme	mussel-inspired	401:415	arg1	MCNF/PDA					451:458	MCNF/PDA	451:458	MCNF/PDA	451:458	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	2	64	theme	mussel-inspired	401:415	arg1	nanocomposite					436:448	a novel mussel-inspired magnetic cellulose nanocomposite	393:448	a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF)	393:504	Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics.					
33169606	4	65	from	activity	788:795	arg1	range					837:841	a wide pH range	827:841	a wide pH range of 2-10 in the presence of H2O2	827:873	The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of H2O2 and have a maximum degradation capacity of 2265 mg/g.					
34188111	4	0	with	infection	743:751	arg1	RV					769:770	RV	769:770	RV	769:770	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	4	0	with	infection	743:751	arg1	rotavirus					758:766	rotavirus	758:766	rotavirus (RV)	758:771	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	1	1	theme	intestinal	151:160	arg1	mucosa					162:167	the intestinal mucosa	147:167	the intestinal mucosa	147:167	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	2	theme	barrier	255:261	arg1	strength					263:270	elevated intestinal barrier strength	235:270	elevated intestinal barrier strength	235:270	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	5	3	theme	microbiota	928:937	arg1	changes					949:955	significant long-lasting microbiota community changes	903:955	significant long-lasting microbiota community changes in the small or large intestine	903:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	0	4	from	alterations	6:16	arg1	composition					47:57	the intestinal microbiota composition	21:57	the intestinal microbiota composition upon Rotavirus infection	21:82	Minor alterations in the intestinal microbiota composition upon Rotavirus infection do not affect susceptibility to DSS colitis.					
34188111	5	5	theme	significant	903:913	arg1	changes					949:955	significant long-lasting microbiota community changes	903:955	significant long-lasting microbiota community changes in the small or large intestine	903:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	1	6	theme	various	358:364	arg1	models					366:371	various models	358:371	various models	358:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	7	from	mucosa	162:167	arg1	triggers					135:142	Viral triggers	129:142	Viral triggers at the intestinal mucosa	129:167	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	5	8	theme	large	973:977	arg1	intestine					979:987	the small or large intestine	960:987	the small or large intestine	960:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	1	9	theme	relative	276:283	arg1	protection					285:294	relative protection	276:294	relative protection from subsequent inflammatory bowel disease (IBD) induction in various models	276:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	0	10	theme	DSS	116:118	arg1	colitis					120:126	DSS colitis	116:126	DSS colitis	116:126	Minor alterations in the intestinal microbiota composition upon Rotavirus infection do not affect susceptibility to DSS colitis.					
34188111	5	11	theme	long-lasting	915:926	arg1	changes					949:955	significant long-lasting microbiota community changes	903:955	significant long-lasting microbiota community changes in the small or large intestine	903:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	6	12	theme	RV	1089:1090	arg1	infection					1092:1100	adult murine RV infection	1076:1100	adult murine RV infection	1076:1100	Hence, adult murine RV infection does not exert lasting effects on intestinal homeostasis.					
34188111	2	13	theme	commensal	470:478	arg1	bacteria					480:487	commensal bacteria	470:487	commensal bacteria	470:487	As viruses can interfere with the intestinal immune system both directly and indirectly through commensal bacteria, cause-effect relationships are difficult to define.					
34188111	2	14	theme	cause-effect	490:501	arg1	relationships					503:515	cause-effect relationships	490:515	cause-effect relationships	490:515	As viruses can interfere with the intestinal immune system both directly and indirectly through commensal bacteria, cause-effect relationships are difficult to define.					
34188111	5	15	theme	sulfate	1037:1043	arg1	colitis					1060:1066	subsequent dextran sulfate sodium-induced colitis	1018:1066	subsequent dextran sulfate sodium-induced colitis	1018:1066	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	5	16	from	changes	949:955	arg1	intestine					979:987	the small or large intestine	960:987	the small or large intestine	960:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	1	17	theme	multiple	178:185	arg1	effects					194:200	multiple global effects	178:200	multiple global effects	178:200	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	0	18	theme	Minor	0:4	arg1	alterations					6:16	Minor alterations	0:16	Minor alterations in the intestinal microbiota composition upon Rotavirus infection	0:82	Minor alterations in the intestinal microbiota composition upon Rotavirus infection do not affect susceptibility to DSS colitis.					
34188111	4	19	theme	IBD	855:857	arg1	severity					843:850	the severity	839:850	the severity of IBD	839:857	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	1	20	theme	global	187:192	arg1	effects					194:200	multiple global effects	178:200	multiple global effects	178:200	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	4	21	theme	intestinal	810:819	arg1	microbiome					821:830	the intestinal microbiome	806:830	the intestinal microbiome	806:830	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	3	22	theme	causative	578:586	arg1	factors					588:594	putatively causative factors	567:594	putatively causative factors	567:594	Due to the complexity of putatively causative factors, our understanding of such virus-mediated protection is currently very limited.					
34188111	5	23	theme	dextran	1029:1035	arg1	sulfate					1037:1043	subsequent dextran sulfate	1018:1043	subsequent dextran sulfate sodium-induced colitis	1018:1066	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	6	24	theme	adult	1076:1080	arg1	infection					1092:1100	adult murine RV infection	1076:1100	adult murine RV infection	1076:1100	Hence, adult murine RV infection does not exert lasting effects on intestinal homeostasis.					
34188111	1	25	theme	subsequent	301:310	arg1	induction					345:353	subsequent inflammatory bowel disease (IBD) induction	301:353	subsequent inflammatory bowel disease (IBD) induction in various models	301:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	3	26	theme	factors	588:594	arg1	complexity					553:562	the complexity	549:562	the complexity of putatively causative factors	549:594	Due to the complexity of putatively causative factors, our understanding of such virus-mediated protection is currently very limited.					
34188111	0	27	theme	microbiota	36:45	arg1	composition					47:57	the intestinal microbiota composition	21:57	the intestinal microbiota composition upon Rotavirus infection	21:82	Minor alterations in the intestinal microbiota composition upon Rotavirus infection do not affect susceptibility to DSS colitis.					
34188111	1	28	theme	inflammatory	312:323	arg1	disease					331:337	inflammatory bowel disease	312:337	subsequent inflammatory bowel disease (IBD) induction in various models	301:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	28	theme	inflammatory	312:323	arg1	IBD					340:342	IBD	340:342	IBD	340:342	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	2	29	theme	immune	419:424	arg1	system					426:431	the intestinal immune system	404:431	the intestinal immune system	404:431	As viruses can interfere with the intestinal immune system both directly and indirectly through commensal bacteria, cause-effect relationships are difficult to define.					
34188111	3	30	theme	such	618:621	arg1	protection					638:647	such virus-mediated protection	618:647	such virus-mediated protection	618:647	Due to the complexity of putatively causative factors, our understanding of such virus-mediated protection is currently very limited.					
34188111	5	31	theme	RV	874:875	arg1	infection					877:885	RV infection	874:885	RV infection	874:885	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	1	32	theme	intestinal	205:214	arg1	integrity					216:224	intestinal integrity	205:224	intestinal integrity	205:224	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	0	33	theme	intestinal	25:34	arg1	composition					47:57	the intestinal microbiota composition	21:57	the intestinal microbiota composition upon Rotavirus infection	21:82	Minor alterations in the intestinal microbiota composition upon Rotavirus infection do not affect susceptibility to DSS colitis.					
34188111	1	34	theme	bowel	325:329	arg1	disease					331:337	inflammatory bowel disease	312:337	subsequent inflammatory bowel disease (IBD) induction in various models	301:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	34	theme	bowel	325:329	arg1	IBD					340:342	IBD	340:342	IBD	340:342	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	35	contain	have	173:176	arg1	triggers					135:142	Viral triggers	129:142	Viral triggers at the intestinal mucosa	129:167	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	35	contain	have	173:176	arg2	effects					194:200	multiple global effects	178:200	multiple global effects	178:200	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	3	36	theme	virus-mediated	623:636	arg1	protection					638:647	such virus-mediated protection	618:647	such virus-mediated protection	618:647	Due to the complexity of putatively causative factors, our understanding of such virus-mediated protection is currently very limited.					
34188111	2	37	theme	intestinal	408:417	arg1	system					426:431	the intestinal immune system	404:431	the intestinal immune system	404:431	As viruses can interfere with the intestinal immune system both directly and indirectly through commensal bacteria, cause-effect relationships are difficult to define.					
34188111	1	38	theme	disease	331:337	arg1	induction					345:353	subsequent inflammatory bowel disease (IBD) induction	301:353	subsequent inflammatory bowel disease (IBD) induction in various models	301:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	5	39	theme	sodium-induced	1045:1058	arg1	colitis					1060:1066	subsequent dextran sulfate sodium-induced colitis	1018:1066	subsequent dextran sulfate sodium-induced colitis	1018:1066	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	6	40	theme	intestinal	1136:1145	arg1	homeostasis					1147:1157	intestinal homeostasis	1136:1157	intestinal homeostasis	1136:1157	Hence, adult murine RV infection does not exert lasting effects on intestinal homeostasis.					
34188111	4	41	theme	enteric	735:741	arg1	infection					743:751	adult enteric infection	729:751	adult enteric infection with rotavirus (RV)	729:771	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	1	42	from	induction	345:353	arg1	strength					263:270	elevated intestinal barrier strength	235:270	elevated intestinal barrier strength	235:270	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	42	from	induction	345:353	arg1	protection					285:294	relative protection	276:294	relative protection from subsequent inflammatory bowel disease (IBD) induction in various models	276:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	1	42	from	induction	345:353	arg1	models					366:371	various models	358:371	various models	358:371	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	5	43	theme	colitis	1060:1066	arg1	severity					1006:1013	the severity	1002:1013	the severity of subsequent dextran sulfate sodium-induced colitis	1002:1066	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	4	44	theme	adult	729:733	arg1	infection					743:751	adult enteric infection	729:751	adult enteric infection with rotavirus (RV)	729:771	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	4	45	theme	microbiome	821:830	arg1	composition					791:801	the composition	787:801	the composition of the intestinal microbiome	787:830	We here set out to better understand the impact that adult enteric infection with rotavirus (RV) might have on the composition of the intestinal microbiome and on the severity of IBD.					
34188111	1	46	theme	Viral	129:133	arg1	triggers					135:142	Viral triggers	129:142	Viral triggers at the intestinal mucosa	129:167	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	5	47	theme	small	964:968	arg1	intestine					979:987	the small or large intestine	960:987	the small or large intestine	960:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	6	48	theme	murine	1082:1087	arg1	infection					1092:1100	adult murine RV infection	1076:1100	adult murine RV infection	1076:1100	Hence, adult murine RV infection does not exert lasting effects on intestinal homeostasis.					
34188111	5	49	theme	community	939:947	arg1	changes					949:955	significant long-lasting microbiota community changes	903:955	significant long-lasting microbiota community changes in the small or large intestine	903:987	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	3	50	theme	protection	638:647	arg1	understanding					601:613	our understanding	597:613	our understanding of such virus-mediated protection	597:647	Due to the complexity of putatively causative factors, our understanding of such virus-mediated protection is currently very limited.					
34188111	1	51	theme	elevated	235:242	arg1	strength					263:270	elevated intestinal barrier strength	235:270	elevated intestinal barrier strength	235:270	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
34188111	0	52	theme	Rotavirus	64:72	arg1	infection					74:82	Rotavirus infection	64:82	Rotavirus infection	64:82	Minor alterations in the intestinal microbiota composition upon Rotavirus infection do not affect susceptibility to DSS colitis.					
34188111	5	53	theme	subsequent	1018:1027	arg1	sulfate					1037:1043	subsequent dextran sulfate	1018:1043	subsequent dextran sulfate sodium-induced colitis	1018:1066	We found that RV infection neither induced significant long-lasting microbiota community changes in the small or large intestine nor affected the severity of subsequent dextran sulfate sodium-induced colitis.					
34188111	6	54	theme	lasting	1117:1123	arg1	effects					1125:1131	lasting effects	1117:1131	lasting effects	1117:1131	Hence, adult murine RV infection does not exert lasting effects on intestinal homeostasis.					
34188111	1	55	theme	intestinal	244:253	arg1	strength					263:270	elevated intestinal barrier strength	235:270	elevated intestinal barrier strength	235:270	Viral triggers at the intestinal mucosa can have multiple global effects on intestinal integrity, causing elevated intestinal barrier strength and relative protection from subsequent inflammatory bowel disease (IBD) induction in various models.					
33172613	0	0	theme	bilirubin	83:91	arg1	removal					93:99	bilirubin removal	83:99	bilirubin removal	83:99	Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.					
33172613	2	1	theme	unique	453:458	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	3	2	from	effects	738:744	arg1	bilirubin					749:757	bilirubin	749:757	bilirubin	749:757	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	3	3	from	time	703:706	arg1	bilirubin					749:757	bilirubin	749:757	bilirubin	749:757	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	1	4	theme	cross-linked	213:224	arg1	polymerization					226:239	in situ cross-linked polymerization	205:239	in situ cross-linked polymerization	205:239	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	3	5	theme	adsorption	680:689	arg1	time					703:706	short adsorption equilibrium time	674:706	short adsorption equilibrium time (2 h)	674:712	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	3	5	theme	adsorption	680:689	arg1	2 h					709:711	2 h	709:711	2 h	709:711	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	5	6	theme	albumin	1083:1089	arg1	presence					1071:1078	presence	1071:1078	presence of albumin	1071:1089	More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin.					
33172613	5	7	theme	adsorption	1041:1050	arg1	rate					1052:1055	high adsorption rate	1036:1055	high adsorption rate (over 70%)	1036:1066	More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin.					
33172613	5	7	theme	adsorption	1041:1050	arg1	%					1065:1065	over 70%	1058:1065	over 70%	1058:1065	More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin.					
33172613	1	8	theme	/soy	141:144	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	8	theme	/soy	141:144	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	6	9	from	efficiency	1172:1181	arg1	devices					1210:1216	self-made hemoperfusion devices	1186:1216	self-made hemoperfusion devices	1186:1216	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	2	10	theme	3D	475:476	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	6	11	theme	adsorption	1161:1170	arg1	efficiency					1172:1181	high dynamic adsorption efficiency	1148:1181	high dynamic adsorption efficiency in self-made hemoperfusion devices	1148:1216	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	3	12	theme	high	622:625	arg1	content					631:637	high SPI content	622:637	high SPI content	622:637	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	6	13	theme	self-made	1186:1194	arg1	devices					1210:1216	self-made hemoperfusion devices	1186:1216	self-made hemoperfusion devices	1186:1216	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	7	14	theme	hemoperfusion	1369:1381	arg1	therapy					1358:1364	therapy	1358:1364	therapy of hemoperfusion	1358:1381	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	4	15	theme	bilirubin	936:944	arg1	concentration					946:958	the initial bilirubin concentration	924:958	the initial bilirubin concentration	924:958	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	2	16	theme	network	486:492	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	1	17	link	cross-linked	213:224	arg1	polymerization					226:239	in situ cross-linked polymerization	205:239	in situ cross-linked polymerization	205:239	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	18	theme	fibrous	478:484	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	7	19	theme	new	1344:1346	arg1	ideas					1348:1352	new ideas	1344:1352	new ideas for therapy of hemoperfusion	1344:1381	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	2	20	theme	AFM	392:394	arg1	assay					430:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	7	21	theme	separation	1398:1407	arg1	process					1409:1415	plasma separation process	1391:1415	plasma separation process	1391:1415	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	1	22	theme	protein	146:152	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	22	theme	protein	146:152	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	23	theme	hemolysis	420:428	arg1	assay					430:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	3	24	theme	equilibrium	691:701	arg1	time					703:706	short adsorption equilibrium time	674:706	short adsorption equilibrium time (2 h)	674:712	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	3	24	theme	equilibrium	691:701	arg1	2 h					709:711	2 h	709:711	2 h	709:711	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	4	25	theme	membrane	810:817	arg1	rate					775:778	The adsorption rate	760:778	The adsorption rate for free bilirubin of BC/SPI5 membrane	760:817	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	2	26	theme	test	411:414	arg1	assay					430:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	1	27	theme	isolate	154:160	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	27	theme	isolate	154:160	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	28	used	used	246:249	arg2	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	28	used	used	246:249	arg2	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	4	29	theme	initial	928:934	arg1	concentration					946:958	the initial bilirubin concentration	924:958	the initial bilirubin concentration	924:958	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	6	30	theme	hemoperfusion	1196:1208	arg1	devices					1210:1216	self-made hemoperfusion devices	1186:1216	self-made hemoperfusion devices	1186:1216	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	0	31	theme	Plant	0:4	arg1	protein					6:12	Plant protein	0:12	Plant protein	0:12	Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.					
33172613	3	32	theme	multiple	718:725	arg1	effects					738:744	multiple adsorption effects	718:744	multiple adsorption effects on bilirubin	718:757	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	2	33	theme	SPI	591:593	arg1	incorporation					574:586	the incorporation	570:586	the incorporation of SPI	570:593	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	7	34	theme	plasma	1391:1396	arg1	process					1409:1415	plasma separation process	1391:1415	plasma separation process	1391:1415	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	3	35	with	membranes	607:615	arg1	content					631:637	high SPI content	622:637	high SPI content	622:637	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	1	36	theme	SPI	163:165	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	36	theme	SPI	163:165	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	0	37	theme	natural	23:29	arg1	cellulose					31:39	natural cellulose	23:39	natural cellulose	23:39	Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.					
33172613	3	38	theme	adsorption	727:736	arg1	effects					738:744	multiple adsorption effects	718:744	multiple adsorption effects on bilirubin	718:757	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	2	39	theme	contact	397:403	arg1	test					411:414	contact angle test	397:414	contact angle test	397:414	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	1	40	theme	efficient	254:262	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	40	theme	efficient	254:262	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	41	dep	in	205:206	arg1	situ					208:211	situ	208:211	situ	208:211	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	42	theme	composite	326:334	arg1	membranes					336:344	The obtained composite membranes	313:344	The obtained composite membranes	313:344	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	1	43	theme	composite	168:176	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	43	theme	composite	168:176	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	3	44	from	efficiency	662:671	arg1	bilirubin					749:757	bilirubin	749:757	bilirubin	749:757	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	1	45	theme	blood	264:268	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	45	theme	blood	264:268	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	46	theme	hydrophobic	506:516	arg1	surfaces					518:525	hydrophobic surfaces	506:525	hydrophobic surfaces	506:525	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	3	47	theme	SPI	627:629	arg1	content					631:637	high SPI content	622:637	high SPI content	622:637	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	7	48	dep	the	1302:1304	arg1	remove					1306:1311	remove	1306:1311	remove	1306:1311	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	7	49	theme	efficient	1278:1286	arg1	absorbent					1288:1296	an economical and efficient absorbent	1260:1296	an economical and efficient absorbent for the remove of bilirubin	1260:1324	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	7	49	theme	efficient	1278:1286	arg1	membrane					1248:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	6	50	theme	composite	1109:1117	arg1	membrane					1119:1126	the composite membrane	1105:1126	the composite membrane	1105:1126	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	1	51	theme	compatible	270:279	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	51	theme	compatible	270:279	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	7	52	link	naturally-derived	1230:1246	arg1	absorbent					1288:1296	an economical and efficient absorbent	1260:1296	an economical and efficient absorbent for the remove of bilirubin	1260:1324	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	7	52	link	naturally-derived	1230:1246	arg1	membrane					1248:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	2	53	theme	angle	405:409	arg1	test					411:414	contact angle test	397:414	contact angle test	397:414	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	2	54	theme	SEM	387:389	arg1	assay					430:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	0	55	theme	adsorption	55:64	arg1	effects					66:72	multiple adsorption effects	46:72	multiple adsorption effects used for bilirubin removal	46:99	Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.					
33172613	2	56	theme	due	563:565	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	4	57	theme	BC/SPI5	802:808	arg1	membrane					810:817	BC/SPI5 membrane	802:817	BC/SPI5 membrane	802:817	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	2	58	theme	obtained	317:324	arg1	membranes					336:344	The obtained composite membranes	313:344	The obtained composite membranes	313:344	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	0	59	theme	multiple	46:53	arg1	effects					66:72	multiple adsorption effects	46:72	multiple adsorption effects used for bilirubin removal	46:99	Plant protein modified natural cellulose with multiple adsorption effects used for bilirubin removal.					
33172613	3	60	theme	high	646:649	arg1	efficiency					662:671	high adsorption efficiency	646:671	high adsorption efficiency	646:671	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	5	61	theme	BC/SPI5	1003:1009	arg1	membrane					1011:1018	the BC/SPI5 membrane	999:1018	the BC/SPI5 membrane	999:1018	More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin.					
33172613	4	62	theme	bilirubin	846:854	arg1	100 mg L-1					874:883	100 mg L-1	874:883	100 mg L-1	874:883	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	4	62	theme	bilirubin	846:854	arg1	concentration					856:868	the bilirubin concentration	842:868	the bilirubin concentration	842:868	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	7	63	theme	naturally-derived	1230:1246	arg1	absorbent					1288:1296	an economical and efficient absorbent	1260:1296	an economical and efficient absorbent for the remove of bilirubin	1260:1324	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	7	63	theme	naturally-derived	1230:1246	arg1	membrane					1248:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	2	64	theme	blood	543:547	arg1	compatibility					549:561	outstanding blood compatibility	531:561	outstanding blood compatibility	531:561	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	3	65	theme	adsorption	651:660	arg1	efficiency					662:671	high adsorption efficiency	646:671	high adsorption efficiency	646:671	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	4	66	theme	adsorption	764:773	arg1	rate					775:778	The adsorption rate	760:778	The adsorption rate for free bilirubin of BC/SPI5 membrane	760:817	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	4	67	theme	free	784:787	arg1	bilirubin					789:797	free bilirubin	784:797	free bilirubin	784:797	The adsorption rate for free bilirubin of BC/SPI5 membrane could reach 78.8% when the bilirubin concentration was 100 mg L-1, while it increased to over 96.5% when the initial bilirubin concentration exceeded 400 mg L-1.					
33172613	1	68	theme	bacterial	117:125	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	68	theme	bacterial	117:125	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	69	theme	outstanding	531:541	arg1	compatibility					549:561	outstanding blood compatibility	531:561	outstanding blood compatibility	531:561	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	7	70	theme	economical	1263:1272	arg1	absorbent					1288:1296	an economical and efficient absorbent	1260:1296	an economical and efficient absorbent for the remove of bilirubin	1260:1324	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	7	70	theme	economical	1263:1272	arg1	membrane					1248:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	3	71	theme	BC/SPI	600:605	arg1	membranes					607:615	The BC/SPI membranes	596:615	The BC/SPI membranes with high SPI content	596:637	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	1	72	theme	cellulose	127:135	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	72	theme	cellulose	127:135	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	73	theme	coated	468:473	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	5	74	theme	high	1036:1039	arg1	rate					1052:1055	high adsorption rate	1036:1055	high adsorption rate (over 70%)	1036:1066	More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin.					
33172613	5	74	theme	high	1036:1039	arg1	%					1065:1065	over 70%	1058:1065	over 70%	1058:1065	More importantly, the BC/SPI5 membrane still exhibited high adsorption rate (over 70%) in presence of albumin.					
33172613	6	75	theme	dynamic	1153:1159	arg1	efficiency					1172:1181	high dynamic adsorption efficiency	1148:1181	high dynamic adsorption efficiency in self-made hemoperfusion devices	1148:1216	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	7	76	theme	novel	1224:1228	arg1	absorbent					1288:1296	an economical and efficient absorbent	1260:1296	an economical and efficient absorbent for the remove of bilirubin	1260:1324	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	7	76	theme	novel	1224:1228	arg1	membrane					1248:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane	1219:1255	This novel naturally-derived membrane is an economical and efficient absorbent for the remove of bilirubin, and will provide new ideas for therapy of hemoperfusion without plasma separation process.					
33172613	2	77	theme	FTIR	381:384	arg1	assay					430:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	FTIR, SEM, AFM, contact angle test and hemolysis assay	381:434	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	1	78	theme	in	205:206	arg1	polymerization					226:239	in situ cross-linked polymerization	205:239	in situ cross-linked polymerization	205:239	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	2	79	theme	protein	460:466	arg1	structures					494:503	unique protein coated 3D fibrous network structures	453:503	unique protein coated 3D fibrous network structures	453:503	The obtained composite membranes were successively characterized by FTIR, SEM, AFM, contact angle test and hemolysis assay, which exhibited unique protein coated 3D fibrous network structures, hydrophobic surfaces and outstanding blood compatibility due to the incorporation of SPI.					
33172613	3	80	theme	short	674:678	arg1	time					703:706	short adsorption equilibrium time	674:706	short adsorption equilibrium time (2 h)	674:712	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	3	80	theme	short	674:678	arg1	2 h					709:711	2 h	709:711	2 h	709:711	The BC/SPI membranes with high SPI content showed high adsorption efficiency, short adsorption equilibrium time (2 h) and multiple adsorption effects on bilirubin.					
33172613	6	81	theme	high	1148:1151	arg1	efficiency					1172:1181	high dynamic adsorption efficiency	1148:1181	high dynamic adsorption efficiency in self-made hemoperfusion devices	1148:1216	Furthermore, the composite membrane could also maintain high dynamic adsorption efficiency in self-made hemoperfusion devices.					
33172613	1	82	theme	BC	138:139	arg1	adsorbents					281:290	efficient blood compatible adsorbents	254:290	efficient blood compatible adsorbents	254:290	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33172613	1	82	theme	BC	138:139	arg1	membranes					178:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes	117:186	In this study, bacterial cellulose (BC)/soy protein isolate (SPI) composite membranes were prepared by in situ cross-linked polymerization, and used as efficient blood compatible adsorbents to remove bilirubin.					
33066426	1	0	theme	κ-carrageenan	341:353	arg1	-and					360:363	κ-carrageenan (CAR)-and	341:363	κ-carrageenan (CAR)-and	341:363	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	1	with	solutions	250:258	arg1	chitosan					402:409	positively charged chitosan	383:409	positively charged chitosan (CS)	383:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	1	with	solutions	250:258	arg1	CS					412:413	CS	412:413	CS	412:413	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	2	theme	bacterial	177:185	arg1	cellulose					187:195	bacterial cellulose	177:195	bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	177:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	3	theme	charged	274:280	arg1	polysaccharides-hyaluronan					282:307	negatively charged polysaccharides-hyaluronan	263:307	negatively charged polysaccharides-hyaluronan (HA)	263:312	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	3	theme	charged	274:280	arg1	HA					310:311	HA	310:311	HA	310:311	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	3	4	theme	impregnated	672:682	arg1	polysaccharides					692:706	impregnated anionic polysaccharides	672:706	impregnated anionic polysaccharides	672:706	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	1	5	theme	cellulose	187:195	arg1	impregnation					161:172	impregnation	161:172	impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	161:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	6	theme	polysaccharides-hyaluronan	282:307	arg1	solutions					250:258	the solutions	246:258	the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	246:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	2	7	theme	polyelectrolyte	517:531	arg1	complex					533:539	a polyelectrolyte complex	515:539	a polyelectrolyte complex	515:539	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex changed the architecture of the BC network.					
33066426	1	8	theme	CAR	356:358	arg1	-and					360:363	κ-carrageenan (CAR)-and	341:363	κ-carrageenan (CAR)-and	341:363	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	4	9	theme	>	871:871	arg1	BC-HA-CS					873:880	BC-ALG-CS > BC-HA-CS	861:880	BC-ALG-CS > BC-HA-CS	861:880	The porosity and swelling of the composites increased in the order: BC-ALG-CS > BC-HA-CS > BC-CAR-CS.					
33066426	5	10	theme	stem	956:959	arg1	cells					961:965	mesenchymal stem cells	944:965	mesenchymal stem cells	944:965	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	4	11	theme	BC-ALG-CS	861:869	arg1	BC-HA-CS					873:880	BC-ALG-CS > BC-HA-CS	861:880	BC-ALG-CS > BC-HA-CS	861:880	The porosity and swelling of the composites increased in the order: BC-ALG-CS > BC-HA-CS > BC-CAR-CS.					
33066426	3	12	with	interaction	772:782	arg1	CS					789:790	CS	789:790	CS	789:790	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	1	13	theme	-and	360:363	arg1	solutions					250:258	the solutions	246:258	the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	246:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	3	14	dep	structure	589:597	arg1	The					585:587	The	585:587	The	585:587	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	4	15	dep	porosity	797:804	arg1	The					793:795	The	793:795	The	793:795	The porosity and swelling of the composites increased in the order: BC-ALG-CS > BC-HA-CS > BC-CAR-CS.					
33066426	4	16	theme	composites	826:835	arg1	swelling					810:817	swelling	810:817	swelling	810:817	The porosity and swelling of the composites increased in the order: BC-ALG-CS > BC-HA-CS > BC-CAR-CS.					
33066426	4	16	theme	composites	826:835	arg1	porosity					797:804	porosity	797:804	porosity	797:804	The porosity and swelling of the composites increased in the order: BC-ALG-CS > BC-HA-CS > BC-CAR-CS.					
33066426	5	17	theme	original	976:983	arg1	sample					988:993	the original BC sample	972:993	the original BC sample	972:993	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	2	18	theme	polysaccharide	440:453	arg1	solutions					455:463	the polysaccharide solutions	436:463	the polysaccharide solutions	436:463	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex changed the architecture of the BC network.					
33066426	2	19	theme	BC	573:574	arg1	network					576:582	the BC network	569:582	the BC network	569:582	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex changed the architecture of the BC network.					
33066426	1	20	theme	Komagataeibacter	209:224	arg1	BC					237:238	BC	237:238	BC	237:238	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	20	theme	Komagataeibacter	209:224	arg1	rhaeticus					226:234	Komagataeibacter rhaeticus	209:234	Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	209:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	0	21	theme	Bacterial	0:8	arg1	Komagataeibacter					21:36	Komagataeibacter	21:36	Komagataeibacter	21:36	Bacterial Cellulose (Komagataeibacter rhaeticus) Biocomposites and Their Cytocompatibility.					
33066426	0	21	theme	Bacterial	0:8	arg1	Cellulose					10:18	Bacterial Cellulose	0:18	Bacterial Cellulose (Komagataeibacter rhaeticus)	0:47	Bacterial Cellulose (Komagataeibacter rhaeticus) Biocomposites and Their Cytocompatibility.					
33066426	3	22	from	polysaccharides	719:733	arg1	turn					738:741	turn	738:741	turn	738:741	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	1	23	theme	novel	104:108	arg1	biocomposites					131:143	novel polysaccharide-based biocomposites	104:143	novel polysaccharide-based biocomposites	104:143	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	24	theme	sodium	315:320	arg1	ALG					332:334	ALG	332:334	ALG	332:334	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	24	theme	sodium	315:320	arg1	alginate					322:329	sodium alginate	315:329	sodium alginate (ALG)	315:335	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	3	25	theme	biocomposites	634:646	arg1	structure					589:597	structure	589:597	structure	589:597	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	3	25	theme	biocomposites	634:646	arg1	morphology					600:609	morphology	600:609	morphology	600:609	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	3	25	theme	biocomposites	634:646	arg1	properties					616:625	properties	616:625	properties	616:625	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	1	26	theme	polysaccharide-based	110:129	arg1	biocomposites					131:143	novel polysaccharide-based biocomposites	104:143	novel polysaccharide-based biocomposites	104:143	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	2	27	theme	BC	474:475	arg1	network					477:483	the BC network	470:483	the BC network	470:483	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex changed the architecture of the BC network.					
33066426	1	28	theme	alginate	322:329	arg1	solutions					250:258	the solutions	246:258	the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	246:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	29	theme	charged	394:400	arg1	chitosan					402:409	positively charged chitosan	383:409	positively charged chitosan (CS)	383:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	1	29	theme	charged	394:400	arg1	CS					412:413	CS	412:413	CS	412:413	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	3	30	theme	interaction	772:782	arg1	nature					758:763	the nature	754:763	the nature of the interaction with CS	754:790	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	5	31	with	biocompatibility	922:937	arg1	cells					961:965	mesenchymal stem cells	944:965	mesenchymal stem cells	944:965	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	0	32	dep	Biocomposites	49:61	arg1	Komagataeibacter					21:36	Komagataeibacter	21:36	Komagataeibacter	21:36	Bacterial Cellulose (Komagataeibacter rhaeticus) Biocomposites and Their Cytocompatibility.					
33066426	0	32	dep	Biocomposites	49:61	arg1	Cellulose					10:18	Bacterial Cellulose	0:18	Bacterial Cellulose (Komagataeibacter rhaeticus)	0:47	Bacterial Cellulose (Komagataeibacter rhaeticus) Biocomposites and Their Cytocompatibility.					
33066426	2	33	theme	network	576:582	arg1	architecture					553:564	the architecture	549:564	the architecture of the BC network	549:582	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex changed the architecture of the BC network.					
33066426	1	34	theme	biocomposites	131:143	arg1	series					94:99	A series	92:99	A series of novel polysaccharide-based biocomposites	92:143	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	2	35	theme	solutions	455:463	arg1	penetration					421:431	The penetration	417:431	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex	417:539	The penetration of the polysaccharide solutions into the BC network and their interaction to form a polyelectrolyte complex changed the architecture of the BC network.					
33066426	1	36	with	rhaeticus	226:234	arg1	solutions					250:258	the solutions	246:258	the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS)	246:414	A series of novel polysaccharide-based biocomposites was obtained by impregnation of bacterial cellulose produced by Komagataeibacter rhaeticus (BC) with the solutions of negatively charged polysaccharides-hyaluronan (HA), sodium alginate (ALG), or κ-carrageenan (CAR)-and subsequently with positively charged chitosan (CS).					
33066426	5	37	theme	best	1037:1040	arg1	characteristics					1042:1056	the best characteristics	1033:1056	the best characteristics	1033:1056	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	5	38	theme	BC	985:986	arg1	sample					988:993	the original BC sample	972:993	the original BC sample	972:993	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	0	39	dep	Komagataeibacter	21:36	arg1	rhaeticus					38:46	Komagataeibacter rhaeticus	21:46	Komagataeibacter rhaeticus	21:46	Bacterial Cellulose (Komagataeibacter rhaeticus) Biocomposites and Their Cytocompatibility.					
33066426	5	40	theme	BC-ALG-CS	1005:1013	arg1	composite					1015:1023	the BC-ALG-CS composite	1001:1023	the BC-ALG-CS composite showing the best characteristics	1001:1056	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	5	41	theme	mesenchymal	944:954	arg1	cells					961:965	mesenchymal stem cells	944:965	mesenchymal stem cells	944:965	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	5	42	theme	higher	915:920	arg1	biocompatibility					922:937	higher biocompatibility	915:937	higher biocompatibility with mesenchymal stem cells than the original BC sample	915:993	The composites show higher biocompatibility with mesenchymal stem cells than the original BC sample, with the BC-ALG-CS composite showing the best characteristics.					
33066426	3	43	theme	anionic	684:690	arg1	polysaccharides					692:706	impregnated anionic polysaccharides	672:706	impregnated anionic polysaccharides	672:706	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
33066426	3	44	theme	polysaccharides	692:706	arg1	type					664:667	the type	660:667	the type of impregnated anionic polysaccharides	660:706	The structure, morphology, and properties of the biocomposites depended on the type of impregnated anionic polysaccharides, and those polysaccharides in turn determined the nature of the interaction with CS.					
32044372	0	0	theme	dyes	96:99	arg1	removal					85:91	removal	85:91	removal of dyes and Cupper(II) from their aqueous mixtures	85:142	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	2	1	theme	single	391:396	arg1	systems					409:415	respective single and binary systems	380:415	respective single and binary systems	380:415	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	9	2	theme	complete	1523:1530	arg1	changes					1532:1538	the complete changes	1519:1538	the complete changes in their surface structures after saturated adsorption	1519:1593	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	6	3	theme	pollutants	912:921	arg1	processes					954:962	spontaneous and exothermal processes	927:962	spontaneous and exothermal processes driven by increasing entropy	927:991	The adsorptions of these three pollutants are spontaneous and exothermal processes driven by increasing entropy.					
32044372	6	3	theme	pollutants	912:921	arg1	adsorptions					885:895	The adsorptions	881:895	The adsorptions of these three pollutants	881:921	The adsorptions of these three pollutants are spontaneous and exothermal processes driven by increasing entropy.					
32044372	2	4	theme	respective	380:389	arg1	systems					409:415	respective single and binary systems	380:415	respective single and binary systems	380:415	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	7	5	theme	pH	1039:1040	arg1	dependence					1042:1051	high pH dependence	1034:1051	high pH dependence	1034:1051	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	1	6	theme	silica-sand/anionized-starch	147:174	arg1	CMS-SS					187:192	CMS-SS	187:192	CMS-SS	187:192	A silica-sand/anionized-starch composite (CMS-SS) was prepared simply.					
32044372	1	6	theme	silica-sand/anionized-starch	147:174	arg1	composite					176:184	A silica-sand/anionized-starch composite	145:184	A silica-sand/anionized-starch composite (CMS-SS)	145:193	A silica-sand/anionized-starch composite (CMS-SS) was prepared simply.					
32044372	0	7	theme	Cupper	105:110	arg1	removal					85:91	removal	85:91	removal of dyes and Cupper(II) from their aqueous mixtures	85:142	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	9	8	theme	due	1512:1514	arg1	water					1506:1510	water	1506:1510	water due to the complete changes in their surface structures after saturated adsorption	1506:1593	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	7	9	theme	high	1034:1037	arg1	dependence					1042:1051	high pH dependence	1034:1051	high pH dependence	1034:1051	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	2	10	theme	dyes	282:285	arg1	removal					262:268	removal	262:268	removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions	262:343	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	3	11	theme	silica	461:466	arg1	sand					468:471	silica sand	461:471	silica sand	461:471	Compared with the anionized-starch without silica sand, CMS-SS shows evidently improved adsorption capacities, i.e. approximately 653.31 ± 27.30, 1246.40 ± 34.10, and 383.08 ± 13.50 mg·g-1, for MB, CV, and Cu(II), respectively, ascribed to the additional carboxyl groups.					
32044372	2	12	theme	cationic	273:280	arg1	dyes					282:285	cationic dyes	273:285	cationic dyes [methyl blue (MB) and crystal violet (CV)]	273:328	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	0	13	from	mixtures	135:142	arg1	removal					85:91	removal	85:91	removal of dyes and Cupper(II) from their aqueous mixtures	85:142	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	2	14	dep	blue	295:298	arg1	MB					301:302	MB	301:302	MB	301:302	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	7	15	theme	CMS-SS	1022:1027	arg1	behaviors					1009:1017	The adsorption behaviors	994:1017	The adsorption behaviors of CMS-SS	994:1027	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	8	16	theme	dye	1212:1214	arg1	adsorption					1198:1207	a preferential adsorption	1183:1207	a preferential adsorption of dye over Cu(II)	1183:1226	Dyes showed higher affinity to CMS-SS than metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture.					
32044372	8	17	theme	aqueous	1237:1243	arg1	mixture					1245:1251	their aqueous mixture	1231:1251	their aqueous mixture	1231:1251	Dyes showed higher affinity to CMS-SS than metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture.					
32044372	7	18	contain	have	1029:1032	arg1	behaviors					1009:1017	The adsorption behaviors	994:1017	The adsorption behaviors of CMS-SS	994:1027	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	7	18	contain	have	1029:1032	arg2	dependence					1042:1051	high pH dependence	1034:1051	high pH dependence	1034:1051	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	8	19	theme	metal	1164:1168	arg1	ions					1170:1173	metal ions	1164:1173	metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture	1164:1251	Dyes showed higher affinity to CMS-SS than metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture.					
32044372	7	20	theme	electrostatic	1058:1070	arg1	attraction					1072:1081	electrostatic attraction	1058:1081	electrostatic attraction	1058:1081	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	9	21	theme	new	1456:1458	arg1	adsorbents					1460:1469	new adsorbents	1456:1469	new adsorbents	1456:1469	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	9	22	from	water	1506:1510	arg1	removal					1475:1481	removal	1475:1481	removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption	1475:1593	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	2	23	theme	ions	340:343	arg1	removal					262:268	removal	262:268	removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions	262:343	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	24	from	water	371:375	arg1	II					353:354	II	353:354	II	353:354	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	24	from	water	371:375	arg1	Cu					358:359	Cu(II)	358:363	Cu(II)	358:363	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	24	from	water	371:375	arg1	[cupper					345:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper	235:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II)	235:355	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	24	from	water	371:375	arg1	CMS-SS					216:221	CMS-SS	216:221	CMS-SS	216:221	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	25	theme	metal	334:338	arg1	ions					340:343	metal ions	334:343	metal ions	334:343	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	4	26	theme	Langmuir	742:749	arg1	suitable					801:808	suitable	801:808	suitable	801:808	The isotherms and kinetics study indicated that the Langmuir model and the pseudo-second-order model were more suitable.					
32044372	4	26	theme	Langmuir	742:749	arg1	model					751:755	the Langmuir model	738:755	the Langmuir model	738:755	The isotherms and kinetics study indicated that the Langmuir model and the pseudo-second-order model were more suitable.					
32044372	9	27	theme	surface	1549:1555	arg1	structures					1557:1566	their surface structures	1543:1566	their surface structures	1543:1566	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	0	28	theme	selective	18:26	arg1	adsorption					28:37	the selective adsorption	14:37	the selective adsorption of silica-sand/anionized-starch composite	14:79	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	4	29	theme	isotherms	694:702	arg1	study					717:721	The isotherms and kinetics study	690:721	The isotherms and kinetics study	690:721	The isotherms and kinetics study indicated that the Langmuir model and the pseudo-second-order model were more suitable.					
32044372	2	30	theme	blue	295:298	arg1	dyes					282:285	cationic dyes	273:285	cationic dyes [methyl blue (MB) and crystal violet (CV)]	273:328	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	4	31	theme	kinetics	708:715	arg1	study					717:721	The isotherms and kinetics study	690:721	The isotherms and kinetics study	690:721	The isotherms and kinetics study indicated that the Langmuir model and the pseudo-second-order model were more suitable.					
32044372	8	32	theme	preferential	1185:1196	arg1	adsorption					1198:1207	a preferential adsorption	1183:1207	a preferential adsorption of dye over Cu(II)	1183:1226	Dyes showed higher affinity to CMS-SS than metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture.					
32044372	7	33	theme	important	1091:1099	arg1	role					1101:1104	an important role	1088:1104	an important role	1088:1104	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	7	34	theme	adsorption	998:1007	arg1	behaviors					1009:1017	The adsorption behaviors	994:1017	The adsorption behaviors of CMS-SS	994:1027	The adsorption behaviors of CMS-SS have high pH dependence, and electrostatic attraction play an important role in adsorption.					
32044372	5	35	theme	monolayer	856:864	arg1	process					826:832	The adsorption process	811:832	The adsorption process	811:832	The adsorption process is thus a homogeneous monolayer chemisorption.					
32044372	5	35	theme	monolayer	856:864	arg1	chemisorption					866:878	a homogeneous monolayer chemisorption	842:878	a homogeneous monolayer chemisorption	842:878	The adsorption process is thus a homogeneous monolayer chemisorption.					
32044372	2	36	used	used	227:230	arg2	CMS-SS					216:221	CMS-SS	216:221	CMS-SS	216:221	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	36	used	used	227:230	arg2	[cupper					345:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper	235:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II)	235:355	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	9	37	theme	silica	1377:1382	arg1	sand					1384:1387	embedded silica sand	1368:1387	embedded silica sand	1368:1387	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	5	38	theme	adsorption	815:824	arg1	process					826:832	The adsorption process	811:832	The adsorption process	811:832	The adsorption process is thus a homogeneous monolayer chemisorption.					
32044372	5	38	theme	adsorption	815:824	arg1	chemisorption					866:878	a homogeneous monolayer chemisorption	842:878	a homogeneous monolayer chemisorption	842:878	The adsorption process is thus a homogeneous monolayer chemisorption.					
32044372	0	39	theme	aqueous	127:133	arg1	mixtures					135:142	their aqueous mixtures	121:142	their aqueous mixtures	121:142	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	9	40	theme	saturated	1574:1582	arg1	adsorption					1584:1593	saturated adsorption	1574:1593	saturated adsorption	1574:1593	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	3	41	theme	adsorption	506:515	arg1	capacities					517:526	evidently improved adsorption capacities	487:526	evidently improved adsorption capacities	487:526	Compared with the anionized-starch without silica sand, CMS-SS shows evidently improved adsorption capacities, i.e. approximately 653.31 ± 27.30, 1246.40 ± 34.10, and 383.08 ± 13.50 mg·g-1, for MB, CV, and Cu(II), respectively, ascribed to the additional carboxyl groups.					
32044372	0	42	theme	adsorption	28:37	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.	0:143	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	3	43	theme	additional	662:671	arg1	groups					682:687	the additional carboxyl groups	658:687	the additional carboxyl groups	658:687	Compared with the anionized-starch without silica sand, CMS-SS shows evidently improved adsorption capacities, i.e. approximately 653.31 ± 27.30, 1246.40 ± 34.10, and 383.08 ± 13.50 mg·g-1, for MB, CV, and Cu(II), respectively, ascribed to the additional carboxyl groups.					
32044372	8	44	theme	higher	1133:1138	arg1	affinity					1140:1147	higher affinity	1133:1147	higher affinity to CMS-SS than metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture	1133:1251	Dyes showed higher affinity to CMS-SS than metal ions causing a preferential adsorption of dye over Cu(II) in their aqueous mixture.					
32044372	9	45	theme	anionic	1489:1495	arg1	dye					1497:1499	an anionic dye	1486:1499	an anionic dye	1486:1499	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	3	46	dep	653.31 ± 27.30	548:561	arg1	i.e.					529:532	i.e.	529:532	i.e.	529:532	Compared with the anionized-starch without silica sand, CMS-SS shows evidently improved adsorption capacities, i.e. approximately 653.31 ± 27.30, 1246.40 ± 34.10, and 383.08 ± 13.50 mg·g-1, for MB, CV, and Cu(II), respectively, ascribed to the additional carboxyl groups.					
32044372	0	47	theme	composite	71:79	arg1	adsorption					28:37	the selective adsorption	14:37	the selective adsorption of silica-sand/anionized-starch composite	14:79	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	3	48	theme	carboxyl	673:680	arg1	groups					682:687	the additional carboxyl groups	658:687	the additional carboxyl groups	658:687	Compared with the anionized-starch without silica sand, CMS-SS shows evidently improved adsorption capacities, i.e. approximately 653.31 ± 27.30, 1246.40 ± 34.10, and 383.08 ± 13.50 mg·g-1, for MB, CV, and Cu(II), respectively, ascribed to the additional carboxyl groups.					
32044372	9	49	theme	dye	1497:1499	arg1	removal					1475:1481	removal	1475:1481	removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption	1475:1593	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	2	50	theme	violet	317:322	arg1	dyes					282:285	cationic dyes	273:285	cationic dyes [methyl blue (MB) and crystal violet (CV)]	273:328	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	0	51	theme	silica-sand/anionized-starch	42:69	arg1	composite					71:79	silica-sand/anionized-starch composite	42:79	silica-sand/anionized-starch composite	42:79	Evaluation of the selective adsorption of silica-sand/anionized-starch composite for removal of dyes and Cupper(II) from their aqueous mixtures.					
32044372	9	52	theme	saturated	1275:1283	arg1	adsorption					1285:1294	saturated adsorption	1275:1294	saturated adsorption	1275:1294	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	3	53	theme	improved	497:504	arg1	capacities					517:526	evidently improved adsorption capacities	487:526	evidently improved adsorption capacities	487:526	Compared with the anionized-starch without silica sand, CMS-SS shows evidently improved adsorption capacities, i.e. approximately 653.31 ± 27.30, 1246.40 ± 34.10, and 383.08 ± 13.50 mg·g-1, for MB, CV, and Cu(II), respectively, ascribed to the additional carboxyl groups.					
32044372	2	54	from	[cupper	345:351	arg1	systems					409:415	respective single and binary systems	380:415	respective single and binary systems	380:415	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	55	theme	crystal	309:315	arg1	CV					325:326	CV	325:326	CV	325:326	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	55	theme	crystal	309:315	arg1	violet					317:322	crystal violet	309:322	crystal violet (CV)	309:327	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	9	56	from	changes	1532:1538	arg1	structures					1557:1566	their surface structures	1543:1566	their surface structures	1543:1566	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	5	57	theme	homogeneous	844:854	arg1	process					826:832	The adsorption process	811:832	The adsorption process	811:832	The adsorption process is thus a homogeneous monolayer chemisorption.					
32044372	5	57	theme	homogeneous	844:854	arg1	chemisorption					866:878	a homogeneous monolayer chemisorption	842:878	a homogeneous monolayer chemisorption	842:878	The adsorption process is thus a homogeneous monolayer chemisorption.					
32044372	6	58	theme	exothermal	943:952	arg1	processes					954:962	spontaneous and exothermal processes	927:962	spontaneous and exothermal processes driven by increasing entropy	927:991	The adsorptions of these three pollutants are spontaneous and exothermal processes driven by increasing entropy.					
32044372	6	58	theme	exothermal	943:952	arg1	adsorptions					885:895	The adsorptions	881:895	The adsorptions of these three pollutants	881:921	The adsorptions of these three pollutants are spontaneous and exothermal processes driven by increasing entropy.					
32044372	2	59	theme	adsorbent	248:256	arg1	II					353:354	II	353:354	II	353:354	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	59	theme	adsorbent	248:256	arg1	Cu					358:359	Cu(II)	358:363	Cu(II)	358:363	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	59	theme	adsorbent	248:256	arg1	[cupper					345:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper	235:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II)	235:355	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	59	theme	adsorbent	248:256	arg1	CMS-SS					216:221	CMS-SS	216:221	CMS-SS	216:221	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	4	60	theme	pseudo-second-order	765:783	arg1	suitable					801:808	suitable	801:808	suitable	801:808	The isotherms and kinetics study indicated that the Langmuir model and the pseudo-second-order model were more suitable.					
32044372	4	60	theme	pseudo-second-order	765:783	arg1	model					785:789	the pseudo-second-order model	761:789	the pseudo-second-order model	761:789	The isotherms and kinetics study indicated that the Langmuir model and the pseudo-second-order model were more suitable.					
32044372	2	61	theme	binary	402:407	arg1	systems					409:415	respective single and binary systems	380:415	respective single and binary systems	380:415	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	62	theme	efficient	238:246	arg1	II					353:354	II	353:354	II	353:354	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	62	theme	efficient	238:246	arg1	Cu					358:359	Cu(II)	358:363	Cu(II)	358:363	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	62	theme	efficient	238:246	arg1	[cupper					345:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper	235:351	an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II)	235:355	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	2	62	theme	efficient	238:246	arg1	CMS-SS					216:221	CMS-SS	216:221	CMS-SS	216:221	CMS-SS was used as an efficient adsorbent for removal of cationic dyes [methyl blue (MB) and crystal violet (CV)] and metal ions [cupper(II), Cu(II)] from water in respective single and binary systems.					
32044372	9	63	theme	embedded	1368:1375	arg1	sand					1384:1387	embedded silica sand	1368:1387	embedded silica sand	1368:1387	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	9	64	theme	recovered	1414:1422	arg1	adsorbents					1424:1433	those rapidly recovered adsorbents	1400:1433	those rapidly recovered adsorbents	1400:1433	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	9	65	theme	enlarged	1345:1352	arg1	density					1354:1360	its enlarged density	1341:1360	its enlarged density after embedded silica sand	1341:1387	This adsorbent after saturated adsorption could be rapidly separated from water due to its enlarged density after embedded silica sand; moreover, those rapidly recovered adsorbents were tried to use as new adsorbents for removal of an anionic dye from water due to the complete changes in their surface structures after saturated adsorption.					
32044372	6	66	theme	spontaneous	927:937	arg1	processes					954:962	spontaneous and exothermal processes	927:962	spontaneous and exothermal processes driven by increasing entropy	927:991	The adsorptions of these three pollutants are spontaneous and exothermal processes driven by increasing entropy.					
32044372	6	66	theme	spontaneous	927:937	arg1	adsorptions					885:895	The adsorptions	881:895	The adsorptions of these three pollutants	881:921	The adsorptions of these three pollutants are spontaneous and exothermal processes driven by increasing entropy.					
32829857	6	0	theme	responsive	1019:1028	arg1	nanoparticles					1030:1042	the proposed pH responsive nanoparticles	1003:1042	the proposed pH responsive nanoparticles	1003:1042	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	2	1	theme	scanning	393:400	arg1	microscopy					411:420	scanning electron microscopy	393:420	scanning electron microscopy	393:420	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	6	2	theme	proposed	1007:1014	arg1	nanoparticles					1030:1042	the proposed pH responsive nanoparticles	1003:1042	the proposed pH responsive nanoparticles	1003:1042	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	6	3	theme	milk	1100:1103	arg1	changes					1113:1119	milk quality changes	1100:1119	milk quality changes	1100:1119	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	0	4	theme	freshness	121:129	arg1	monitoring					131:140	milk freshness monitoring	116:140	milk freshness monitoring	116:140	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	1	5	theme	weak	267:270	arg1	environment					279:289	a weak acidic environment	265:289	a weak acidic environment	265:289	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	0	6	theme	milk	116:119	arg1	monitoring					131:140	milk freshness monitoring	116:140	milk freshness monitoring	116:140	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	4	7	theme	%	747:747	arg1	rate					735:738	a retention rate	723:738	a retention rate of ∼50 % after 15 d	723:758	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	1	8	theme	acidic	272:277	arg1	environment					279:289	a weak acidic environment	265:289	a weak acidic environment	265:289	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	6	9	theme	reference	1138:1146	arg1	value					1148:1152	some reference value	1133:1152	some reference value for future related research	1133:1180	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	6	10	theme	quality	1105:1111	arg1	changes					1113:1119	milk quality changes	1100:1119	milk quality changes	1100:1119	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	4	11	theme	retention	725:733	arg1	rate					735:738	a retention rate	723:738	a retention rate of ∼50 % after 15 d	723:758	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	5	12	theme	spoiled	828:834	arg1	milk					836:839	spoiled milk	828:839	spoiled milk	828:839	When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change.					
32829857	5	13	theme	milk	812:815	arg1	freshness					817:825	the milk freshness	808:825	the milk freshness	808:825	When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change.					
32829857	3	14	theme	loading	483:489	arg1	efficiencies					491:502	loading efficiencies	483:502	loading efficiencies	483:502	The loading efficiencies and oxidation resistances of the proposed substances were measured.					
32829857	6	15	theme	related	1165:1171	arg1	research					1173:1180	future related research	1158:1180	future related research	1158:1180	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	1	16	theme	wall	194:197	arg1	chitosan					182:189	chitosan	182:189	chitosan	182:189	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	1	16	theme	wall	194:197	arg1	materials					199:207	wall materials	194:207	wall materials	194:207	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	1	16	theme	wall	194:197	arg1	gelatin					170:176	gelatin	170:176	gelatin	170:176	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	5	17	used	used	789:792	arg2	nanoparticles					770:782	the nanoparticles	766:782	the nanoparticles	766:782	When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change.					
32829857	6	18	theme	future	1158:1163	arg1	research					1173:1180	future related research	1158:1180	future related research	1158:1180	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	5	19	theme	milk	895:898	arg1	color					880:884	the color	876:884	the color of fresh milk	876:898	When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change.					
32829857	1	20	with	composites	214:223	arg1	capability					251:260	a controlled release capability	230:260	a controlled release capability	230:260	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	6	21	theme	pH	1016:1017	arg1	nanoparticles					1030:1042	the proposed pH responsive nanoparticles	1003:1042	the proposed pH responsive nanoparticles	1003:1042	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	3	22	theme	oxidation	508:516	arg1	resistances					518:528	oxidation resistances	508:528	oxidation resistances	508:528	The loading efficiencies and oxidation resistances of the proposed substances were measured.					
32829857	2	23	theme	nanoparticles	464:476	arg1	properties					446:455	the properties	442:455	the properties of the nanoparticles	442:476	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	4	24	theme	loading	627:633	arg1	efficiency					635:644	a loading efficiency	625:644	a loading efficiency of 83.81 %	625:655	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	4	25	theme	storage	681:687	arg1	capacity					689:696	suitable long-term storage capacity	662:696	suitable long-term storage capacity	662:696	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	0	26	theme	gelatin/chitosan	40:55	arg1	nanoparticles					57:69	dynamic gelatin/chitosan nanoparticles	32:69	dynamic gelatin/chitosan nanoparticles	32:69	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	3	27	theme	substances	546:555	arg1	efficiencies					491:502	loading efficiencies	483:502	loading efficiencies	483:502	The loading efficiencies and oxidation resistances of the proposed substances were measured.					
32829857	3	27	theme	substances	546:555	arg1	resistances					518:528	oxidation resistances	508:528	oxidation resistances	508:528	The loading efficiencies and oxidation resistances of the proposed substances were measured.					
32829857	4	28	theme	room	701:704	arg1	temperature					706:716	room temperature	701:716	room temperature	701:716	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	0	29	theme	dynamic	32:38	arg1	nanoparticles					57:69	dynamic gelatin/chitosan nanoparticles	32:69	dynamic gelatin/chitosan nanoparticles	32:69	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	5	30	theme	fresh	889:893	arg1	milk					895:898	fresh milk	889:898	fresh milk	889:898	When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change.					
32829857	4	31	theme	long-term	671:679	arg1	capacity					689:696	suitable long-term storage capacity	662:696	suitable long-term storage capacity	662:696	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	1	32	from	composites	214:223	arg1	environment					279:289	a weak acidic environment	265:289	a weak acidic environment	265:289	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	6	33	theme	dynamic	1078:1084	arg1	monitoring					1086:1095	the dynamic monitoring	1074:1095	the dynamic monitoring of milk quality changes	1074:1119	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	4	34	theme	optimal	578:584	arg1	conditions					586:595	optimal conditions	578:595	optimal conditions	578:595	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	3	35	theme	proposed	537:544	arg1	substances					546:555	the proposed substances	533:555	the proposed substances	533:555	The loading efficiencies and oxidation resistances of the proposed substances were measured.					
32829857	0	36	theme	nanoparticles	57:69	arg1	optimization					16:27	optimization	16:27	optimization	16:27	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	0	36	theme	nanoparticles	57:69	arg1	Development					0:10	Development	0:10	Development	0:10	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	1	37	theme	protecting	324:333	arg1	anthocyanins					335:346	protecting anthocyanins	324:346	protecting anthocyanins	324:346	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	2	38	theme	infrared	367:374	arg1	spectroscopy					376:387	Fourier-transform infrared spectroscopy	349:387	Fourier-transform infrared spectroscopy	349:387	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	6	39	from	chroma	965:970	arg1	milk					975:978	milk	975:978	milk	975:978	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	3	40	dep	efficiencies	491:502	arg1	The					479:481	The	479:481	The	479:481	The loading efficiencies and oxidation resistances of the proposed substances were measured.					
32829857	2	41	theme	Fourier-transform	349:365	arg1	spectroscopy					376:387	Fourier-transform infrared spectroscopy	349:387	Fourier-transform infrared spectroscopy	349:387	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	6	42	theme	changes	1113:1119	arg1	monitoring					1086:1095	the dynamic monitoring	1074:1095	the dynamic monitoring of milk quality changes	1074:1119	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	1	43	theme	controlled	232:241	arg1	capability					251:260	a controlled release capability	230:260	a controlled release capability	230:260	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	0	44	theme	blueberry	89:97	arg1	anthocyanins					99:110	blueberry anthocyanins	89:110	blueberry anthocyanins for milk freshness monitoring	89:140	Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.					
32829857	2	45	used	used	427:430	arg2	microscopy					411:420	scanning electron microscopy	393:420	scanning electron microscopy	393:420	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	2	45	used	used	427:430	arg2	spectroscopy					376:387	Fourier-transform infrared spectroscopy	349:387	Fourier-transform infrared spectroscopy	349:387	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	1	46	theme	release	243:249	arg1	capability					251:260	a controlled release capability	230:260	a controlled release capability	230:260	In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins.					
32829857	4	47	theme	%	655:655	arg1	efficiency					635:644	a loading efficiency	625:644	a loading efficiency of 83.81 %	625:655	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	4	47	theme	%	655:655	arg1	capacity					689:696	suitable long-term storage capacity	662:696	suitable long-term storage capacity	662:696	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
32829857	5	48	theme	reddish	853:859	arg1	color					861:865	a reddish color	851:865	a reddish color	851:865	When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change.					
32829857	2	49	theme	electron	402:409	arg1	microscopy					411:420	scanning electron microscopy	393:420	scanning electron microscopy	393:420	Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles.					
32829857	6	50	theme	stable	958:963	arg1	chroma					965:970	a stable chroma	956:970	a stable chroma in milk for 0-16 h.	956:990	Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.					
32829857	4	51	theme	suitable	662:669	arg1	capacity					689:696	suitable long-term storage capacity	662:696	suitable long-term storage capacity	662:696	Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d.					
34216657	8	0	theme	extracellular	1263:1275	arg1	matrix					1277:1282	the extracellular matrix	1259:1282	the extracellular matrix	1259:1282	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	9	1	theme	broad	1395:1399	arg1	investigation					1408:1420	a broad safety investigation	1393:1420	a broad safety investigation	1393:1420	Additionally, a broad safety investigation found no evidence of adverse effects after GA-AuNRs treatment.					
34216657	10	2	theme	triple	1581:1586	arg1	melanomas					1598:1606	advanced triple wild-type melanomas	1572:1606	advanced triple wild-type melanomas	1572:1606	Therefore, this study unprecedentedly reports GA-AuNRs as a potential nanomedicine for advanced triple wild-type melanomas.					
34216657	7	3	theme	superficial	1089:1099	arg1	depth					1117:1121	superficial lung metastasis depth	1089:1121	superficial lung metastasis depth	1089:1121	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	1	4	theme	genetic	157:163	arg1	groups					180:185	common genetic classificatory groups	150:185	common genetic classificatory groups	150:185	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	8	5	theme	melanoma	1226:1233	arg1	cells					1235:1239	melanoma cells	1226:1239	melanoma cells ability to invade the extracellular matrix and grow into colonies	1226:1305	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	0	6	theme	mistreated	76:85	arg1	melanomas					87:95	mistreated melanomas	76:95	mistreated melanomas	76:95	Biocompatible gum arabic-gold nanorod composite as an effective therapy for mistreated melanomas.					
34216657	9	7	theme	safety	1401:1406	arg1	investigation					1408:1420	a broad safety investigation	1393:1420	a broad safety investigation	1393:1420	Additionally, a broad safety investigation found no evidence of adverse effects after GA-AuNRs treatment.					
34216657	10	8	theme	advanced	1572:1579	arg1	melanomas					1598:1606	advanced triple wild-type melanomas	1572:1606	advanced triple wild-type melanomas	1572:1606	Therefore, this study unprecedentedly reports GA-AuNRs as a potential nanomedicine for advanced triple wild-type melanomas.					
34216657	1	9	theme	classificatory	165:178	arg1	groups					180:185	common genetic classificatory groups	150:185	common genetic classificatory groups	150:185	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	10	10	theme	potential	1545:1553	arg1	nanomedicine					1555:1566	a potential nanomedicine	1543:1566	a potential nanomedicine for advanced triple wild-type melanomas	1543:1606	Therefore, this study unprecedentedly reports GA-AuNRs as a potential nanomedicine for advanced triple wild-type melanomas.					
34216657	10	10	theme	potential	1545:1553	arg1	GA-AuNRs					1531:1538	GA-AuNRs	1531:1538	GA-AuNRs	1531:1538	Therefore, this study unprecedentedly reports GA-AuNRs as a potential nanomedicine for advanced triple wild-type melanomas.					
34216657	3	11	theme	antitumor	406:414	arg1	properties					416:425	the intrinsic antitumor properties	392:425	the intrinsic antitumor properties of gold nanoparticles	392:447	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	3	12	theme	gold	430:433	arg1	nanoparticles					435:447	gold nanoparticles	430:447	gold nanoparticles	430:447	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	7	13	theme	better	1052:1057	arg1	prognosis					1059:1067	better prognosis	1052:1067	better prognosis	1052:1067	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	8	14	dep	effect	1349:1354	arg1	addition					1311:1318	addition	1311:1318	addition	1311:1318	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	9	15	theme	effects	1451:1457	arg1	evidence					1431:1438	no evidence	1428:1438	no evidence of adverse effects	1428:1457	Additionally, a broad safety investigation found no evidence of adverse effects after GA-AuNRs treatment.					
34216657	9	16	theme	adverse	1443:1449	arg1	effects					1451:1457	adverse effects	1443:1457	adverse effects	1443:1457	Additionally, a broad safety investigation found no evidence of adverse effects after GA-AuNRs treatment.					
34216657	6	17	theme	melanoma-bearing	887:902	arg1	mice					904:907	treating triple wild-type melanoma-bearing mice	861:907	treating triple wild-type melanoma-bearing mice	861:907	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	6	18	theme	wild-type	877:885	arg1	mice					904:907	treating triple wild-type melanoma-bearing mice	861:907	treating triple wild-type melanoma-bearing mice	861:907	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	8	19	theme	gum	1367:1369	arg1	arabic					1371:1376	gum arabic	1367:1376	gum arabic	1367:1376	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	3	20	theme	intrinsic	396:404	arg1	properties					416:425	the intrinsic antitumor properties	392:425	the intrinsic antitumor properties of gold nanoparticles	392:447	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	5	21	theme	natural	684:690	arg1	arabic					704:709	The natural polymer gum arabic	680:709	The natural polymer gum arabic	680:709	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	5	21	theme	natural	684:690	arg1	essential					786:794	essential	786:794	essential	786:794	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	6	22	theme	triple	870:875	arg1	mice					904:907	treating triple wild-type melanoma-bearing mice	861:907	treating triple wild-type melanoma-bearing mice	861:907	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	2	23	theme	adverse	307:313	arg1	effects					315:321	devastating adverse effects	295:321	devastating adverse effects	295:321	Chemotherapy and immunotherapy show unsatisfactory results and devastating adverse effects for these called triple wild-type patients.					
34216657	3	24	theme	nanoparticles	435:447	arg1	properties					416:425	the intrinsic antitumor properties	392:425	the intrinsic antitumor properties of gold nanoparticles	392:447	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	3	25	theme	New	367:369	arg1	approaches					371:380	New approaches	367:380	New approaches exploring the intrinsic antitumor properties of gold nanoparticles	367:447	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	5	26	theme	polymer	692:698	arg1	arabic					704:709	The natural polymer gum arabic	680:709	The natural polymer gum arabic	680:709	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	5	26	theme	polymer	692:698	arg1	essential					786:794	essential	786:794	essential	786:794	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	6	27	theme	treating	861:868	arg1	mice					904:907	treating triple wild-type melanoma-bearing mice	861:907	treating triple wild-type melanoma-bearing mice	861:907	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	1	28	theme	effective	192:200	arg1	options					223:229	effective and safe therapeutic options	192:229	effective and safe therapeutic options	192:229	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	0	29	theme	Biocompatible	0:12	arg1	gum					14:16	Biocompatible gum	0:16	Biocompatible gum	0:16	Biocompatible gum arabic-gold nanorod composite as an effective therapy for mistreated melanomas.					
34216657	4	30	theme	arabic-functionalized	598:618	arg1	GA-AuNRs					635:642	GA-AuNRs	635:642	GA-AuNRs	635:642	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	4	30	theme	arabic-functionalized	598:618	arg1	nanorods					625:632	gum arabic-functionalized gold nanorods	594:632	gum arabic-functionalized gold nanorods (GA-AuNRs)	594:643	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	5	31	theme	gum	700:702	arg1	arabic					704:709	The natural polymer gum arabic	680:709	The natural polymer gum arabic	680:709	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	5	31	theme	gum	700:702	arg1	essential					786:794	essential	786:794	essential	786:794	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	7	32	theme	histological	1014:1025	arg1	features					1027:1034	tumor histological features	1008:1034	tumor histological features associated with better prognosis	1008:1067	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	1	33	theme	Advanced	98:105	arg1	patients					116:123	Advanced melanoma patients	98:123	Advanced melanoma patients that are not included in common genetic classificatory groups	98:185	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	3	34	theme	safer	482:486	arg1	alternative					507:517	a safer and more effective alternative	480:517	a safer and more effective alternative	480:517	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	3	34	theme	safer	482:486	arg1	scenario					468:475	this scenario	463:475	this scenario	463:475	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	4	35	theme	gum	594:596	arg1	GA-AuNRs					635:642	GA-AuNRs	635:642	GA-AuNRs	635:642	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	4	35	theme	gum	594:596	arg1	nanorods					625:632	gum arabic-functionalized gold nanorods	594:632	gum arabic-functionalized gold nanorods (GA-AuNRs)	594:643	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	2	36	theme	triple	340:345	arg1	patients					357:364	these called triple wild-type patients	327:364	these called triple wild-type patients	327:364	Chemotherapy and immunotherapy show unsatisfactory results and devastating adverse effects for these called triple wild-type patients.					
34216657	10	37	theme	wild-type	1588:1596	arg1	melanomas					1598:1606	advanced triple wild-type melanomas	1572:1606	advanced triple wild-type melanomas	1572:1606	Therefore, this study unprecedentedly reports GA-AuNRs as a potential nanomedicine for advanced triple wild-type melanomas.					
34216657	1	38	theme	safe	206:209	arg1	options					223:229	effective and safe therapeutic options	192:229	effective and safe therapeutic options	192:229	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	2	39	theme	wild-type	347:355	arg1	patients					357:364	these called triple wild-type patients	327:364	these called triple wild-type patients	327:364	Chemotherapy and immunotherapy show unsatisfactory results and devastating adverse effects for these called triple wild-type patients.					
34216657	2	40	theme	called	333:338	arg1	patients					357:364	these called triple wild-type patients	327:364	these called triple wild-type patients	327:364	Chemotherapy and immunotherapy show unsatisfactory results and devastating adverse effects for these called triple wild-type patients.					
34216657	1	41	theme	therapeutic	211:221	arg1	options					223:229	effective and safe therapeutic options	192:229	effective and safe therapeutic options	192:229	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	4	42	theme	gold	620:623	arg1	GA-AuNRs					635:642	GA-AuNRs	635:642	GA-AuNRs	635:642	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	4	42	theme	gold	620:623	arg1	nanorods					625:632	gum arabic-functionalized gold nanorods	594:632	gum arabic-functionalized gold nanorods (GA-AuNRs)	594:643	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	9	43	theme	GA-AuNRs	1465:1472	arg1	treatment					1474:1482	GA-AuNRs treatment	1465:1482	GA-AuNRs treatment	1465:1482	Additionally, a broad safety investigation found no evidence of adverse effects after GA-AuNRs treatment.					
34216657	3	44	theme	effective	497:505	arg1	alternative					507:517	a safer and more effective alternative	480:517	a safer and more effective alternative	480:517	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	3	44	theme	effective	497:505	arg1	scenario					468:475	this scenario	463:475	this scenario	463:475	New approaches exploring the intrinsic antitumor properties of gold nanoparticles might reverse this scenario as a safer and more effective alternative.					
34216657	4	45	theme	composite	576:584	arg1	safety					564:569	safety	564:569	safety	564:569	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	4	45	theme	composite	576:584	arg1	efficacy					551:558	efficacy	551:558	efficacy	551:558	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	6	46	theme	tumor	957:961	arg1	growth					963:968	primary tumor growth	949:968	primary tumor growth	949:968	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	7	47	theme	intrapulmonary	1144:1157	arg1	metastasis					1159:1168	intrapulmonary metastasis	1144:1168	intrapulmonary metastasis	1144:1168	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	0	48	theme	effective	54:62	arg1	therapy					64:70	an effective therapy	51:70	an effective therapy for mistreated melanomas	51:95	Biocompatible gum arabic-gold nanorod composite as an effective therapy for mistreated melanomas.					
34216657	7	49	theme	metastasis	1159:1168	arg1	depth					1117:1121	superficial lung metastasis depth	1089:1121	superficial lung metastasis depth	1089:1121	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	7	49	theme	metastasis	1159:1168	arg1	incidence					1131:1139	the incidence	1127:1139	the incidence of intrapulmonary metastasis	1127:1168	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	6	50	theme	primary	949:955	arg1	growth					963:968	primary tumor growth	949:968	primary tumor growth	949:968	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	8	51	theme	likely	1325:1330	arg1	effect					1349:1354	a likely immunomodulatory effect	1323:1354	a likely immunomodulatory effect induced by gum arabic	1323:1376	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	6	52	theme	In	831:832	arg1	results					839:845	In vivo results	831:845	In vivo results obtained from treating triple wild-type melanoma-bearing mice	831:907	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
34216657	0	53	dep	nanorod	30:36	arg1	composite					38:46	composite	38:46	composite	38:46	Biocompatible gum arabic-gold nanorod composite as an effective therapy for mistreated melanomas.					
34216657	2	54	theme	devastating	295:305	arg1	effects					315:321	devastating adverse effects	295:321	devastating adverse effects	295:321	Chemotherapy and immunotherapy show unsatisfactory results and devastating adverse effects for these called triple wild-type patients.					
34216657	8	55	theme	cells	1235:1239	arg1	ability					1241:1247	melanoma cells ability	1226:1247	melanoma cells ability to invade the extracellular matrix and grow into colonies	1226:1305	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	1	56	theme	melanoma	107:114	arg1	patients					116:123	Advanced melanoma patients	98:123	Advanced melanoma patients that are not included in common genetic classificatory groups	98:185	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	4	57	theme	wild-type	660:668	arg1	melanoma					670:677	triple wild-type melanoma	653:677	triple wild-type melanoma	653:677	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	5	58	theme	biological	755:764	arg1	environment					766:776	the biological environment	751:776	the biological environment	751:776	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	7	59	theme	lung	1101:1104	arg1	depth					1117:1121	superficial lung metastasis depth	1089:1121	superficial lung metastasis depth	1089:1121	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	4	60	theme	triple	653:658	arg1	melanoma					670:677	triple wild-type melanoma	653:677	triple wild-type melanoma	653:677	Therefore, we investigated the efficacy and safety of a composite made of gum arabic-functionalized gold nanorods (GA-AuNRs) against triple wild-type melanoma.					
34216657	7	61	theme	metastasis	1106:1115	arg1	depth					1117:1121	superficial lung metastasis depth	1089:1121	superficial lung metastasis depth	1089:1121	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	7	62	theme	tumor	1008:1012	arg1	features					1027:1034	tumor histological features	1008:1034	tumor histological features associated with better prognosis	1008:1067	Furthermore, GA-AuNRs induced tumor histological features associated with better prognosis while also reducing superficial lung metastasis depth and the incidence of intrapulmonary metastasis.					
34216657	5	63	from	nanorods	739:746	arg1	environment					766:776	the biological environment	751:776	the biological environment	751:776	The natural polymer gum arabic successfully stabilized the nanorods in the biological environment and was essential to improve their biocompatibility.					
34216657	8	64	theme	immunomodulatory	1332:1347	arg1	effect					1349:1354	a likely immunomodulatory effect	1323:1354	a likely immunomodulatory effect induced by gum arabic	1323:1376	GA-AuNRs' efficacy comes from their capacity to reduce melanoma cells ability to invade the extracellular matrix and grow into colonies, in addition to a likely immunomodulatory effect induced by gum arabic.					
34216657	2	65	theme	unsatisfactory	268:281	arg1	results					283:289	unsatisfactory results	268:289	unsatisfactory results	268:289	Chemotherapy and immunotherapy show unsatisfactory results and devastating adverse effects for these called triple wild-type patients.					
34216657	1	66	theme	common	150:155	arg1	groups					180:185	common genetic classificatory groups	150:185	common genetic classificatory groups	150:185	Advanced melanoma patients that are not included in common genetic classificatory groups lack effective and safe therapeutic options.					
34216657	6	67	dep	In	831:832	arg1	vivo					834:837	vivo	834:837	vivo	834:837	In vivo results obtained from treating triple wild-type melanoma-bearing mice showed that GA-AuNRs remarkably reduced primary tumor growth by 45%.					
33263222	0	0	theme	cisternae	63:71	arg1	perturbation					41:52	perturbation	41:52	perturbation of Golgi cisternae	41:71	Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.					
33263222	4	1	theme	calcium/calcium	834:848	arg1	channels					864:871	calcium/calcium permeable ion channels	834:871	calcium/calcium permeable ion channels	834:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	2	from	disruption	751:760	arg1	genes					765:769	genes	765:769	genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels	765:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	2	from	disruption	751:760	arg1	proteins					783:790	proteins	783:790	proteins with annotated function as or homology to calcium/calcium permeable ion channels	783:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	1	3	theme	stacks	134:139	arg1	organization					108:119	The structural organization	93:119	The structural organization of the Golgi stacks in mammalian cells	93:158	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	0	4	theme	Golgi	57:61	arg1	cisternae					63:71	Golgi cisternae	57:71	Golgi cisternae	57:71	Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.					
33263222	6	5	theme	pathway	1066:1072	arg1	nature					1042:1047	the robust nature	1031:1047	the robust nature of the secretory pathway in P. pastoris	1031:1087	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	4	6	theme	irreversible	667:678	arg1	perturbations					680:692	irreversible perturbations	667:692	irreversible perturbations in the stacking of the Golgi cisternae	667:731	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	6	7	theme	other	1246:1250	arg1	organisms					1252:1260	other organisms	1246:1260	other organisms	1246:1260	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	0	8	theme	Pichia	76:81	arg1	pastoris					83:90	Pichia pastoris	76:90	Pichia pastoris	76:90	Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.					
33263222	6	9	theme	significant	1163:1173	arg1	role					1175:1178	a significant role	1161:1178	a significant role for Golgi stacking	1161:1197	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	4	10	theme	ion	860:862	arg1	channels					864:871	calcium/calcium permeable ion channels	834:871	calcium/calcium permeable ion channels	834:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	6	11	theme	Golgi	1184:1188	arg1	stacking					1190:1197	Golgi stacking	1184:1197	Golgi stacking	1184:1197	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	5	12	theme	ER	930:931	arg1	size					933:936	ER size	930:936	ER size	930:936	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	4	13	theme	cisternae	723:731	arg1	stacking					701:708	the stacking	697:708	the stacking of the Golgi cisternae	697:731	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	1	14	from	eukaryotes	272:281	arg1	clear					257:261	clear	257:261	clear	257:261	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	1	14	from	eukaryotes	272:281	arg1	role					230:233	the role	226:233	the role of morphology	226:247	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	6	15	theme	secretory	1056:1064	arg1	pathway					1066:1072	the secretory pathway	1052:1072	the secretory pathway	1052:1072	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	1	16	theme	morphology	238:247	arg1	clear					257:261	clear	257:261	clear	257:261	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	1	16	theme	morphology	238:247	arg1	role					230:233	the role	226:233	the role of morphology	226:247	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	1	17	from	clear	257:261	arg1	eukaryotes					272:281	lower eukaryotes	266:281	lower eukaryotes	266:281	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	6	18	from	nature	1042:1047	arg1	pastoris					1080:1087	P. pastoris	1077:1087	P. pastoris	1077:1087	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	1	19	theme	mammalian	144:152	arg1	cells					154:158	mammalian cells	144:158	mammalian cells	144:158	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	0	20	theme	CSC1-2	27:32	arg1	Knockout					0:7	Knockout	0:7	Knockout	0:7	Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.					
33263222	1	21	from	organization	108:119	arg1	cells					154:158	mammalian cells	144:158	mammalian cells	144:158	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	5	22	theme	whole	939:943	arg1	glycomics					950:958	whole cell glycomics	939:958	whole cell glycomics	939:958	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	2	23	theme	Golgi	357:361	arg1	function					385:392	secretory pathway function	367:392	secretory pathway function	367:392	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	2	23	theme	Golgi	357:361	arg1	organization					337:348	the structural organization	322:348	the structural organization of the Golgi	322:361	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	4	24	with	strains	654:660	arg1	perturbations					680:692	irreversible perturbations	667:692	irreversible perturbations in the stacking of the Golgi cisternae	667:731	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	25	theme	Golgi	717:721	arg1	cisternae					723:731	the Golgi cisternae	713:731	the Golgi cisternae	713:731	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	6	26	theme	robust	1035:1040	arg1	nature					1042:1047	the robust nature	1031:1047	the robust nature of the secretory pathway in P. pastoris	1031:1087	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	4	27	theme	or	819:820	arg1	homology					822:829	or homology	819:829	or homology to calcium/calcium permeable ion channels	819:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	5	28	theme	secretory	908:916	arg1	pathway					918:924	the secretory pathway	904:924	the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans	904:989	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	6	29	theme	Ca2+	1106:1109	arg1	concentration					1111:1123	Ca2+ concentration	1106:1123	Ca2+ concentration	1106:1123	Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.					
33263222	5	30	theme	protein	975:981	arg1	glycans					983:989	recombinant protein glycans	963:989	recombinant protein glycans	963:989	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	2	31	theme	Pichia	400:405	arg1	system					427:432	a model system	419:432	a model system	419:432	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	2	31	theme	Pichia	400:405	arg1	pastoris					407:414	Pichia pastoris	400:414	Pichia pastoris	400:414	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	3	32	theme	secretory	514:522	arg1	pathway					524:530	the secretory pathway	510:530	the secretory pathway	510:530	To unstack the Golgi cisternae, we disrupted 18 genes encoding proteins in the secretory pathway without loss of viability.					
33263222	4	33	theme	electron	614:621	arg1	biosensors					565:574	biosensors	565:574	biosensors	565:574	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	33	theme	electron	614:621	arg1	microscopy					623:632	transmission electron microscopy	601:632	transmission electron microscopy	601:632	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	3	34	theme	viability	548:556	arg1	loss					540:543	loss	540:543	loss of viability	540:556	To unstack the Golgi cisternae, we disrupted 18 genes encoding proteins in the secretory pathway without loss of viability.					
33263222	2	35	theme	pathway	377:383	arg1	function					385:392	secretory pathway function	367:392	secretory pathway function	367:392	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	0	36	theme	TVP18	18:22	arg1	Knockout					0:7	Knockout	0:7	Knockout	0:7	Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.					
33263222	1	37	theme	lower	266:270	arg1	eukaryotes					272:281	lower eukaryotes	266:281	lower eukaryotes	266:281	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	4	38	with	proteins	783:790	arg1	function					807:814	annotated function	797:814	annotated function as or homology to calcium/calcium permeable ion channels	797:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	2	39	theme	secretory	367:375	arg1	function					385:392	secretory pathway function	367:392	secretory pathway function	367:392	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	4	40	contain	had	747:749	arg2	disruption					751:760	disruption	751:760	disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels	751:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	40	contain	had	747:749	arg1	strains					654:660	three strains	648:660	three strains	648:660	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	40	contain	had	747:749	arg1	all					734:736	all	734:736	all	734:736	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	41	theme	annotated	797:805	arg1	function					807:814	annotated function	797:814	annotated function as or homology to calcium/calcium permeable ion channels	797:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	3	42	theme	Golgi	450:454	arg1	cisternae					456:464	the Golgi cisternae	446:464	the Golgi cisternae	446:464	To unstack the Golgi cisternae, we disrupted 18 genes encoding proteins in the secretory pathway without loss of viability.					
33263222	2	43	theme	model	421:425	arg1	system					427:432	a model system	419:432	a model system	419:432	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	2	43	theme	model	421:425	arg1	pastoris					407:414	Pichia pastoris	400:414	Pichia pastoris	400:414	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	5	44	from	variation	891:899	arg1	pathway					918:924	the secretory pathway	904:924	the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans	904:989	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	1	45	theme	structural	97:106	arg1	organization					108:119	The structural organization	93:119	The structural organization of the Golgi stacks in mammalian cells	93:158	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	5	46	theme	recombinant	963:973	arg1	glycans					983:989	recombinant protein glycans	963:989	recombinant protein glycans	963:989	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	1	47	attach	linked	177:182	arg1	function					187:194	function	187:194	function	187:194	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	1	47	attach	linked	177:182	arg2	organization					108:119	The structural organization	93:119	The structural organization of the Golgi stacks in mammalian cells	93:158	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	5	48	theme	cell	945:948	arg1	glycomics					950:958	whole cell glycomics	939:958	whole cell glycomics	939:958	Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed.					
33263222	4	49	dep	identified	637:646	arg1	Using					559:563	Using	559:563	Using biosensors, confocal microscopy and transmission electron microscopy	559:632	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	1	50	theme	Golgi	128:132	arg1	stacks					134:139	the Golgi stacks	124:139	the Golgi stacks	124:139	The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes.					
33263222	4	51	theme	permeable	850:858	arg1	channels					864:871	calcium/calcium permeable ion channels	834:871	calcium/calcium permeable ion channels	834:871	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	52	theme	transmission	601:612	arg1	biosensors					565:574	biosensors	565:574	biosensors	565:574	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	52	theme	transmission	601:612	arg1	microscopy					623:632	transmission electron microscopy	601:632	transmission electron microscopy	601:632	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	53	theme	confocal	577:584	arg1	biosensors					565:574	biosensors	565:574	biosensors	565:574	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	4	53	theme	confocal	577:584	arg1	microscopy					586:595	confocal microscopy	577:595	confocal microscopy	577:595	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
33263222	0	54	theme	RSN1	12:15	arg1	Knockout					0:7	Knockout	0:7	Knockout	0:7	Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.					
33263222	2	55	theme	structural	326:335	arg1	organization					337:348	the structural organization	322:348	the structural organization of the Golgi	322:361	Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system.					
33263222	4	56	from	perturbations	680:692	arg1	stacking					701:708	the stacking	697:708	the stacking of the Golgi cisternae	697:731	Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels.					
32428561	8	0	theme	genes	1221:1225	arg1	significance					1184:1195	the significance	1180:1195	the significance of mucin and mucin-like genes in insect growth and development	1180:1258	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	7	1	theme	structural	1084:1093	arg1	integrity					1095:1103	the structural integrity	1080:1103	the structural integrity of the wing cuticle	1080:1123	Suppression of Lmmucin-17 greatly impaired the structural integrity of the wing cuticle during nymph-adult molting.					
32428561	2	2	theme	biological	329:338	arg1	processes					340:348	many biological processes	324:348	many biological processes	324:348	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	4	3	theme	adult	738:742	arg1	development					749:759	adult wing development	738:759	adult wing development	738:759	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	1	4	theme	proline	221:227	arg1	residues					229:236	proline residues	221:236	proline residues	221:236	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	6	5	theme	peritrophic	1005:1015	arg1	membrane					1017:1024	the peritrophic membrane	1001:1024	the peritrophic membrane in midgut	1001:1034	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	2	6	theme	many	324:327	arg1	processes					340:348	many biological processes	324:348	many biological processes	324:348	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	1	7	theme	residues	229:236	arg1	proportion					184:193	a higher proportion	175:193	a higher proportion of threonine, serine, and proline residues in their sequences	175:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	0	8	theme	locust	81:86	arg1	development					52:62	development	52:62	development	52:62	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	0	8	theme	locust	81:86	arg1	growth					41:46	growth	41:46	growth	41:46	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	6	9	from	membrane	1017:1024	arg1	midgut					1029:1034	midgut	1029:1034	midgut	1029:1034	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	3	10	theme	mucin-like	511:520	arg1	genes					522:526	eight mucin or mucin-like genes	496:526	eight mucin or mucin-like genes	496:526	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	0	11	theme	migratory	71:79	arg1	locust					81:86	the migratory locust	67:86	the migratory locust	67:86	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	0	11	theme	migratory	71:79	arg1	migratoria					97:106	Locusta migratoria	89:106	Locusta migratoria	89:106	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	6	12	theme	LmIIM3	945:950	arg1	Deficiency					931:940	Deficiency	931:940	Deficiency of LmIIM3	931:950	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	0	13	theme	Locusta	89:95	arg1	locust					81:86	the migratory locust	67:86	the migratory locust	67:86	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	0	13	theme	Locusta	89:95	arg1	migratoria					97:106	Locusta migratoria	89:106	Locusta migratoria	89:106	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	4	14	theme	migratoria	686:695	arg1	nymphs					697:702	L. migratoria nymphs	683:702	L. migratoria nymphs	683:702	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	7	15	theme	Lmmucin-17	1052:1061	arg1	Suppression					1037:1047	Suppression	1037:1047	Suppression of Lmmucin-17	1037:1061	Suppression of Lmmucin-17 greatly impaired the structural integrity of the wing cuticle during nymph-adult molting.					
32428561	6	16	from	midgut	1029:1034	arg1	defects					990:996	defects	990:996	defects of the peritrophic membrane in midgut	990:1034	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	3	17	theme	mucin	502:506	arg1	genes					522:526	eight mucin or mucin-like genes	496:526	eight mucin or mucin-like genes	496:526	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	8	18	theme	mucin-like	1210:1219	arg1	genes					1221:1225	mucin and mucin-like genes	1200:1225	genes	1221:1225	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	5	19	theme	gastric	867:873	arg1	caeca					875:879	the gastric caeca	863:879	the gastric caeca in which cells were detached from cell junctions	863:928	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	2	20	dep	such	406:409	arg1	as					411:412	as	411:412	as	411:412	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	4	21	theme	RNA	573:575	arg1	interference					577:588	RNA interference	573:588	RNA interference against these genes	573:608	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	4	22	theme	Lmmucin	634:640	arg1	genes					642:646	three Lmmucin genes	628:646	three Lmmucin genes	628:646	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	0	23	theme	family	6:11	arg1	genes					13:17	Mucin family genes	0:17	Mucin family genes	0:17	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	1	24	from	sequences	247:255	arg1	proportion					184:193	a higher proportion	175:193	a higher proportion of threonine, serine, and proline residues in their sequences	175:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	6	25	from	defects	990:996	arg1	midgut					1029:1034	midgut	1029:1034	midgut	1029:1034	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	3	26	theme	migratory	535:543	arg1	migratoria					561:570	Locusta migratoria	553:570	Locusta migratoria	553:570	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	3	26	theme	migratory	535:543	arg1	locust					545:550	the migratory locust	531:550	the migratory locust	531:550	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	0	27	theme	Mucin	0:4	arg1	genes					13:17	Mucin family genes	0:17	Mucin family genes	0:17	Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria.					
32428561	5	28	theme	observed	844:851	arg1	defect					853:858	an observed defect	841:858	an observed defect of the gastric caeca in which cells were detached from cell junctions	841:928	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	5	29	theme	Lmhemomucin	783:793	arg1	knockdown					770:778	knockdown	770:778	knockdown of Lmhemomucin and Lmmucin-12	770:808	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	1	30	theme	higher	177:182	arg1	proportion					184:193	a higher proportion	175:193	a higher proportion of threonine, serine, and proline residues in their sequences	175:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	7	31	theme	nymph-adult	1132:1142	arg1	molting					1144:1150	nymph-adult molting	1132:1150	nymph-adult molting	1132:1150	Suppression of Lmmucin-17 greatly impaired the structural integrity of the wing cuticle during nymph-adult molting.					
32428561	4	32	theme	wing	744:747	arg1	development					749:759	adult wing development	738:759	adult wing development	738:759	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	5	33	theme	cell	915:918	arg1	junctions					920:928	cell junctions	915:928	cell junctions	915:928	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	8	34	theme	insect	1230:1235	arg1	growth					1237:1242	growth	1237:1242	growth	1237:1242	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	7	35	theme	wing	1112:1115	arg1	cuticle					1117:1123	the wing cuticle	1108:1123	the wing cuticle	1108:1123	Suppression of Lmmucin-17 greatly impaired the structural integrity of the wing cuticle during nymph-adult molting.					
32428561	8	36	from	significance	1184:1195	arg1	growth					1237:1242	growth	1237:1242	growth	1237:1242	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	8	36	from	significance	1184:1195	arg1	development					1248:1258	development	1248:1258	development	1248:1258	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	6	37	theme	membrane	1017:1024	arg1	defects					990:996	defects	990:996	defects of the peritrophic membrane in midgut	990:1034	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	5	38	theme	lethal	817:822	arg1	phenotypes					824:833	lethal phenotypes	817:833	lethal phenotypes	817:833	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	7	39	theme	cuticle	1117:1123	arg1	integrity					1095:1103	the structural integrity	1080:1103	the structural integrity of the wing cuticle	1080:1123	Suppression of Lmmucin-17 greatly impaired the structural integrity of the wing cuticle during nymph-adult molting.					
32428561	1	40	from	residues	229:236	arg1	sequences					247:255	their sequences	241:255	their sequences	241:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	8	41	theme	insect	1283:1288	arg1	locust					1290:1295	the orthopteran insect locust	1267:1295	the orthopteran insect locust	1267:1295	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	8	41	theme	insect	1283:1288	arg1	model					1302:1306	a model	1300:1306	a model	1300:1306	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	2	42	from	roles	357:361	arg1	development					377:387	development	377:387	development	377:387	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	2	42	from	roles	357:361	arg1	growth					366:371	growth	366:371	growth	366:371	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	1	43	theme	threonine	198:206	arg1	proportion					184:193	a higher proportion	175:193	a higher proportion of threonine, serine, and proline residues in their sequences	175:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	8	44	theme	mucin	1200:1204	arg1	genes					1221:1225	mucin and mucin-like genes	1200:1225	genes	1221:1225	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	1	45	gly	glycosylated	127:138	arg1	Mucins					109:114	Mucins	109:114	Mucins	109:114	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	1	45	gly	glycosylated	127:138	arg1	proteins					140:147	highly glycosylated proteins	120:147	highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences	120:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	1	46	from	threonine	198:206	arg1	sequences					247:255	their sequences	241:255	their sequences	241:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	3	47	theme	bioinformatic	458:470	arg1	analyses					472:479	bioinformatic analyses	458:479	bioinformatic analyses	458:479	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	3	48	theme	Locusta	553:559	arg1	migratoria					561:570	Locusta migratoria	553:570	Locusta migratoria	553:570	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	3	48	theme	Locusta	553:559	arg1	locust					545:550	the migratory locust	531:550	the migratory locust	531:550	Based on bioinformatic analyses, we identified eight mucin or mucin-like genes in the migratory locust, Locusta migratoria.					
32428561	2	49	from	growth	366:371	arg1	insects					417:423	insects	417:423	insects	417:423	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	2	49	from	growth	366:371	arg1	invertebrates					392:404	invertebrates	392:404	invertebrates such as in insects	392:423	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	4	50	theme	L.	683:684	arg1	nymphs					697:702	L. migratoria nymphs	683:702	L. migratoria nymphs	683:702	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	8	51	theme	present	1157:1163	arg1	study					1165:1169	The present study	1153:1169	The present study	1153:1169	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	1	52	from	proportion	184:193	arg1	sequences					247:255	their sequences	241:255	their sequences	241:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	8	53	theme	orthopteran	1271:1281	arg1	locust					1290:1295	the orthopteran insect locust	1267:1295	the orthopteran insect locust	1267:1295	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	8	53	theme	orthopteran	1271:1281	arg1	model					1302:1306	a model	1300:1306	a model	1300:1306	The present study revealed the significance of mucin and mucin-like genes in insect growth and development, using the orthopteran insect locust as a model.					
32428561	1	54	from	serine	209:214	arg1	sequences					247:255	their sequences	241:255	their sequences	241:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	5	55	theme	caeca	875:879	arg1	defect					853:858	an observed defect	841:858	an observed defect of the gastric caeca in which cells were detached from cell junctions	841:928	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	2	56	from	development	377:387	arg1	invertebrates					392:404	invertebrates	392:404	invertebrates such as in insects	392:423	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	4	57	theme	nymphs	697:702	arg1	survival					671:678	the survival	667:678	the survival of L. migratoria nymphs	667:702	RNA interference against these genes demonstrated that three Lmmucin genes were essential for the survival of L. migratoria nymphs, and one Lmmucin was required for adult wing development.					
32428561	2	58	from	mucins	267:272	arg1	humans					277:282	humans	277:282	humans	277:282	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	2	58	from	mucins	267:272	arg1	vertebrates					288:298	vertebrates	288:298	vertebrates	288:298	Although mucins in humans and vertebrates have been implicated in many biological processes, their roles in growth and development in invertebrates such as in insects remain largely unknown.					
32428561	1	59	theme	serine	209:214	arg1	proportion					184:193	a higher proportion	175:193	a higher proportion of threonine, serine, and proline residues in their sequences	175:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	5	60	theme	Lmmucin-12	799:808	arg1	knockdown					770:778	knockdown	770:778	knockdown of Lmhemomucin and Lmmucin-12	770:808	Indeed, knockdown of Lmhemomucin and Lmmucin-12 caused lethal phenotypes, with an observed defect of the gastric caeca in which cells were detached from cell junctions.					
32428561	6	61	theme	nymphs	977:982	arg1	lethality					964:972	lethality	964:972	lethality of nymphs	964:982	Deficiency of LmIIM3 resulted in lethality of nymphs, with defects of the peritrophic membrane in midgut.					
32428561	1	62	theme	glycosylated	127:138	arg1	Mucins					109:114	Mucins	109:114	Mucins	109:114	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
32428561	1	62	theme	glycosylated	127:138	arg1	proteins					140:147	highly glycosylated proteins	120:147	highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences	120:255	Mucins are highly glycosylated proteins that are characterized by a higher proportion of threonine, serine, and proline residues in their sequences.					
33535117	5	0	theme	stationary	1227:1236	arg1	phase					1238:1242	the stationary phase	1223:1242	the stationary phase	1223:1242	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	7	1	theme	observed	1544:1551	arg1	fractionation					1576:1588	observed intramolecular isotope fractionation	1544:1588	observed intramolecular isotope fractionation	1544:1588	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	1	2	theme	fractionation	290:302	arg1	columns					407:413	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	8	3	from	impact	1749:1754	arg1	composition					1787:1797	the intramolecular isotopic composition	1759:1797	the intramolecular isotopic composition of organic compounds isolated by chromatography methods	1759:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	5	4	theme	phase	1238:1242	arg1	structure					1210:1218	the chemical structure	1197:1218	the chemical structure of the stationary phase	1197:1242	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	4	5	theme	isotope	932:938	arg1	effects					940:946	both 13C and 15N isotope effects	915:946	effects	940:946	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	5	6	theme	fractionation	1161:1173	arg1	profiles					1175:1182	very different intramolecular isotope fractionation profiles	1123:1182	very different intramolecular isotope fractionation profiles	1123:1182	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	4	7	theme	stationary	1025:1034	arg1	phases					1036:1041	different stationary phases	1015:1041	different stationary phases	1015:1041	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	7	8	theme	intermolecular	1617:1630	arg1	interactions					1632:1643	involved intermolecular interactions	1608:1643	involved intermolecular interactions	1608:1643	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	6	9	theme	non-covalent	1372:1383	arg1	interactions					1385:1396	the non-covalent interactions	1368:1396	the non-covalent interactions involved in the migration process	1368:1430	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	7	10	theme	isotope	1568:1574	arg1	fractionation					1576:1588	observed intramolecular isotope fractionation	1544:1588	observed intramolecular isotope fractionation	1544:1588	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	0	11	theme	paracetamol	98:108	arg1	migration					85:93	the migration	81:93	the migration of paracetamol in solid matrices during liquid chromatography	81:155	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	8	12	theme	isotopic	1778:1785	arg1	composition					1787:1797	the intramolecular isotopic composition	1759:1797	the intramolecular isotopic composition of organic compounds isolated by chromatography methods	1759:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	0	13	from	abundance	55:63	arg1	effects					36:42	Intramolecular non-covalent isotope effects	0:42	Intramolecular non-covalent isotope effects at natural abundance	0:63	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	2	14	used	used	447:450	arg2	chromatography					423:436	Liquid chromatography	416:436	Liquid chromatography	416:436	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	4	15	theme	study	857:861	arg1	aim					845:847	The aim	841:847	The aim of this study	841:861	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	1	16	theme	Nuclear	196:202	arg1	Resonance					213:221	Nuclear Magnetic Resonance	196:221	Nuclear Magnetic Resonance spectrometry	196:234	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	0	17	theme	solid	113:117	arg1	matrices					119:126	solid matrices	113:126	solid matrices	113:126	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	3	18	theme	organic	797:803	arg1	contaminants					805:816	organic contaminants	797:816	organic contaminants	797:816	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	2	19	theme	isotopic	555:562	arg1	composition					564:574	the isotopic composition	551:574	the isotopic composition of target compounds	551:594	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	2	20	theme	purification	514:525	arg1	step					527:530	this purification step	509:530	this purification step	509:530	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	1	21	theme	Magnetic	204:211	arg1	Resonance					213:221	Nuclear Magnetic Resonance	196:221	Nuclear Magnetic Resonance spectrometry	196:234	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	0	22	theme	non-covalent	15:26	arg1	effects					36:42	Intramolecular non-covalent isotope effects	0:42	Intramolecular non-covalent isotope effects at natural abundance	0:63	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	3	23	theme	contaminants	805:816	arg1	fate					789:792	fate	789:792	fate	789:792	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	3	23	theme	contaminants	805:816	arg1	transport					775:783	transport	775:783	transport	775:783	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	4	24	theme	15N	928:930	arg1	effects					940:946	both 13C and 15N isotope effects	915:946	effects	940:946	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	8	25	theme	phase	1696:1700	arg1	nature					1671:1676	the nature	1667:1676	the nature of the stationary phase through which the substrate passes	1667:1735	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	5	26	theme	chemical	1201:1208	arg1	structure					1210:1218	the chemical structure	1197:1218	the chemical structure of the stationary phase	1197:1242	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	1	27	theme	Resonance	213:221	arg1	spectrometry					223:234	Nuclear Magnetic Resonance spectrometry	196:234	Nuclear Magnetic Resonance spectrometry	196:234	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	0	28	theme	Intramolecular	0:13	arg1	effects					36:42	Intramolecular non-covalent isotope effects	0:42	Intramolecular non-covalent isotope effects at natural abundance	0:63	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	3	29	dep	transport	775:783	arg1	the					771:773	the	771:773	the	771:773	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	5	30	theme	isotope	1153:1159	arg1	profiles					1175:1182	very different intramolecular isotope fractionation profiles	1123:1182	very different intramolecular isotope fractionation profiles	1123:1182	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	3	31	from	transport	775:783	arg1	environment					828:838	the environment	824:838	the environment	824:838	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	3	32	theme	transport	741:749	arg1	models					751:756	reactive transport models	732:756	reactive transport models that predict the transport and fate of organic contaminants to in the environment	732:838	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	7	33	theme	theoretical	1453:1463	arg1	calculation					1465:1475	theoretical calculation	1453:1475	theoretical calculation performed during this study	1453:1503	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	0	34	theme	liquid	135:140	arg1	chromatography					142:155	liquid chromatography	135:155	liquid chromatography	135:155	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	6	35	theme	interactions	1385:1396	arg1	nature					1358:1363	the nature	1354:1363	the nature of the non-covalent interactions involved in the migration process	1354:1430	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	0	36	theme	isotope	28:34	arg1	effects					36:42	Intramolecular non-covalent isotope effects	0:42	Intramolecular non-covalent isotope effects at natural abundance	0:63	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	1	37	theme	organic	337:343	arg1	substrates					345:354	organic substrates	337:354	organic substrates	337:354	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	2	38	theme	measured	628:635	arg1	data					637:640	the later measured data	618:640	the later measured data	618:640	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	3	39	theme	reactive	732:739	arg1	models					751:756	reactive transport models	732:756	reactive transport models that predict the transport and fate of organic contaminants to in the environment	732:838	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	7	40	theme	direct	1524:1529	arg1	link					1531:1534	the direct link	1520:1534	the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions	1520:1643	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	3	41	from	chromatography	673:686	arg1	results					653:659	results	653:659	results from liquid chromatography	653:686	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	2	42	theme	isotope	488:494	arg1	analysis					496:503	their isotope analysis	482:503	their isotope analysis	482:503	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	0	43	theme	natural	47:53	arg1	abundance					55:63	natural abundance	47:63	natural abundance	47:63	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	1	44	theme	substrates	345:354	arg1	migration					324:332	the migration	320:332	the migration of organic substrates through different stationary phases	320:390	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	2	45	theme	later	622:626	arg1	data					637:640	the later measured data	618:640	the later measured data	618:640	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	0	46	from	migration	85:93	arg1	matrices					119:126	solid matrices	113:126	solid matrices	113:126	Intramolecular non-covalent isotope effects at natural abundance associated with the migration of paracetamol in solid matrices during liquid chromatography.					
33535117	8	47	theme	intramolecular	1763:1776	arg1	composition					1787:1797	the intramolecular isotopic composition	1759:1797	the intramolecular isotopic composition of organic compounds isolated by chromatography methods	1759:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	2	48	theme	prior	473:477	arg1	compounds					463:471	compounds	463:471	compounds prior to their isotope analysis	463:503	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	7	49	theme	involved	1608:1615	arg1	interactions					1632:1643	involved intermolecular interactions	1608:1643	involved intermolecular interactions	1608:1643	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	4	50	theme	isotope	889:895	arg1	analysis					897:904	intramolecular isotope analysis	874:904	intramolecular isotope analysis	874:904	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	8	51	theme	organic	1802:1808	arg1	compounds					1810:1818	organic compounds	1802:1818	organic compounds isolated by chromatography methods	1802:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	3	52	from	fate	789:792	arg1	environment					828:838	the environment	824:838	the environment	824:838	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	1	53	theme	different	364:372	arg1	phases					385:390	different stationary phases	364:390	different stationary phases	364:390	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	8	54	theme	stationary	1685:1694	arg1	phase					1696:1700	the stationary phase	1681:1700	the stationary phase	1681:1700	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	6	55	theme	isotope	1328:1334	arg1	effects					1336:1342	measured isotope effects	1319:1342	measured isotope effects	1319:1342	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	8	56	contain	has	1737:1739	arg1	nature					1671:1676	the nature	1667:1676	the nature of the stationary phase through which the substrate passes	1667:1735	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	8	56	contain	has	1737:1739	arg2	impact					1749:1754	a major impact	1741:1754	a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods	1741:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	2	57	theme	Liquid	416:421	arg1	chromatography					423:436	Liquid chromatography	416:436	Liquid chromatography	416:436	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	1	58	theme	stationary	374:383	arg1	phases					385:390	different stationary phases	364:390	different stationary phases	364:390	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	6	59	theme	migration	1414:1422	arg1	process					1424:1430	the migration process	1410:1430	the migration process	1410:1430	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	3	60	theme	liquid	666:671	arg1	chromatography					673:686	liquid chromatography	666:686	liquid chromatography	666:686	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	6	61	theme	measured	1319:1326	arg1	effects					1336:1342	measured isotope effects	1319:1342	measured isotope effects	1319:1342	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	4	62	theme	paracetamol	979:989	arg1	elution					968:974	the elution	964:974	the elution of paracetamol (acetaminophen)	964:1005	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	5	63	theme	different	1128:1136	arg1	profiles					1175:1182	very different intramolecular isotope fractionation profiles	1123:1182	very different intramolecular isotope fractionation profiles	1123:1182	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	3	64	theme	sorption	702:709	arg1	parameters					711:720	the sorption parameters	698:720	the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment	698:838	Moreover, results from liquid chromatography can yield the sorption parameters needed in reactive transport models that predict the transport and fate of organic contaminants to in the environment.					
33535117	6	65	theme	effects	1336:1342	arg1	distribution					1303:1314	the distribution	1299:1314	the distribution of measured isotope effects	1299:1342	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	6	65	theme	effects	1336:1342	arg1	amplitude					1285:1293	the amplitude	1281:1293	the amplitude	1281:1293	The data also demonstrate that both the amplitude and the distribution of measured isotope effects depend on the nature of the non-covalent interactions involved in the migration process.					
33535117	8	66	theme	major	1743:1747	arg1	impact					1749:1754	a major impact	1741:1754	a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods	1741:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	7	67	theme	intramolecular	1553:1566	arg1	fractionation					1576:1588	observed intramolecular isotope fractionation	1544:1588	observed intramolecular isotope fractionation	1544:1588	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	1	68	dep	fractionation	290:302	arg1	chromatography					392:405	chromatography	392:405	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	4	69	theme	different	1015:1023	arg1	phases					1036:1041	different stationary phases	1015:1041	different stationary phases	1015:1041	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	5	70	theme	intramolecular	1138:1151	arg1	profiles					1175:1182	very different intramolecular isotope fractionation profiles	1123:1182	very different intramolecular isotope fractionation profiles	1123:1182	Results showed very different intramolecular isotope fractionation profiles depending on the chemical structure of the stationary phase.					
33535117	1	71	theme	13C	262:264	arg1	columns					407:413	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	2	72	theme	compounds	586:594	arg1	composition					564:574	the isotopic composition	551:574	the isotopic composition of target compounds	551:594	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	2	73	from	bias	610:613	arg1	data					637:640	the later measured data	618:640	the later measured data	618:640	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	4	74	theme	intramolecular	874:887	arg1	analysis					897:904	intramolecular isotope analysis	874:904	intramolecular isotope analysis	874:904	The aim of this study was to use intramolecular isotope analysis to study both 13C and 15N isotope effects associated with the elution of paracetamol (acetaminophen) through different stationary phases and to compare them to effects observed previously for vanillin.					
33535117	1	75	theme	Position-specific	158:174	arg1	analysis					184:191	Position-specific isotope analysis	158:191	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry	158:234	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	8	76	theme	compounds	1810:1818	arg1	composition					1787:1797	the intramolecular isotopic composition	1759:1797	the intramolecular isotopic composition of organic compounds isolated by chromatography methods	1759:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	1	77	theme	intramolecular	266:279	arg1	fractionation					290:302	intramolecular isotopic fractionation	266:302	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	2	78	theme	target	579:584	arg1	compounds					586:594	target compounds	579:594	target compounds	579:594	Liquid chromatography is often used to isolate compounds prior to their isotope analysis and this purification step potentially alters the isotopic composition of target compounds introducing a bias in the later measured data.					
33535117	7	79	theme	interactions	1632:1643	arg1	nature					1598:1603	the nature	1594:1603	the nature of involved intermolecular interactions	1594:1643	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	7	79	theme	interactions	1632:1643	arg1	fractionation					1576:1588	observed intramolecular isotope fractionation	1544:1588	observed intramolecular isotope fractionation	1544:1588	Results provided by theoretical calculation performed during this study also confirmed the direct link between observed intramolecular isotope fractionation and the nature of involved intermolecular interactions.					
33535117	1	80	theme	isotope	176:182	arg1	analysis					184:191	Position-specific isotope analysis	158:191	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry	158:234	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
33535117	8	81	theme	chromatography	1832:1845	arg1	methods					1847:1853	chromatography methods	1832:1853	chromatography methods	1832:1853	It is concluded that the nature of the stationary phase through which the substrate passes has a major impact on the intramolecular isotopic composition of organic compounds isolated by chromatography methods.					
33535117	1	82	theme	isotopic	281:288	arg1	fractionation					290:302	intramolecular isotopic fractionation	266:302	the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns	258:413	Position-specific isotope analysis by Nuclear Magnetic Resonance spectrometry was employed to study the 13C intramolecular isotopic fractionation associated with the migration of organic substrates through different stationary phases chromatography columns.					
34076032	6	0	theme	gel	1083:1085	arg1	formation					1087:1095	the gel formation	1079:1095	the gel formation	1079:1095	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	8	1	theme	supramolecular	1619:1632	arg1	system					1648:1653	the supramolecular self-assembly system	1615:1653	the supramolecular self-assembly system in biomolecular recognition and response	1615:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	2	theme	physiological	246:258	arg1	autoimmunity					340:351	autoimmunity	340:351	autoimmunity	340:351	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	2	theme	physiological	246:258	arg1	development					303:313	neural development	296:313	neural development	296:313	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	2	theme	physiological	246:258	arg1	processes					277:285	physiological and pathological processes	246:285	physiological and pathological processes	246:285	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	2	theme	physiological	246:258	arg1	interactions					326:337	cell-cell interactions	316:337	cell-cell interactions	316:337	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	2	theme	physiological	246:258	arg1	cancers					357:363	cancers	357:363	cancers	357:363	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	8	3	theme	system	1648:1653	arg1	superiority					1600:1610	the unique superiority	1589:1610	the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response	1589:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	4	theme	pathological	264:275	arg1	autoimmunity					340:351	autoimmunity	340:351	autoimmunity	340:351	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	4	theme	pathological	264:275	arg1	development					303:313	neural development	296:313	neural development	296:313	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	4	theme	pathological	264:275	arg1	processes					277:285	physiological and pathological processes	246:285	physiological and pathological processes	246:285	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	4	theme	pathological	264:275	arg1	interactions					326:337	cell-cell interactions	316:337	cell-cell interactions	316:337	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	4	theme	pathological	264:275	arg1	cancers					357:363	cancers	357:363	cancers	357:363	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	6	5	theme	response	1155:1162	arg1	system					1164:1169	the gel dynamic response system	1139:1169	the gel dynamic response system	1139:1169	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	1	6	attach	attached	165:172	arg1	surfaces					182:189	cell surfaces	177:189	cell surfaces	177:189	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	6	attach	attached	165:172	arg2	acids					108:112	Sialic acids	101:112	Sialic acids located at the terminal end of glycans	101:151	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	7	theme	cell	177:180	arg1	surfaces					182:189	cell surfaces	177:189	cell surfaces	177:189	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	6	8	theme	gel	1143:1145	arg1	system					1164:1169	the gel dynamic response system	1139:1169	the gel dynamic response system	1139:1169	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	3	9	theme	response	571:578	arg1	system					580:585	a fluorescent dynamic response system	549:585	a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel	549:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	7	10	theme	ordered	1387:1393	arg1	accumulation					1395:1406	the ordered accumulation	1383:1406	the ordered accumulation of the gelators	1383:1422	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	8	11	from	superiority	1600:1610	arg1	recognition					1671:1681	biomolecular recognition	1658:1681	biomolecular recognition	1658:1681	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	8	11	from	superiority	1600:1610	arg1	response					1687:1694	response	1687:1694	response	1687:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	3	12	theme	fluorescent	551:561	arg1	system					580:585	a fluorescent dynamic response system	549:585	a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel	549:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	5	13	theme	acid	845:848	arg1	introduction					810:821	introduction	810:821	introduction of N-acetylneuraminic acid (a typical sialic acid)	810:872	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	9	14	theme	promising	1717:1725	arg1	prospects					1727:1735	the promising prospects	1713:1735	the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis	1713:1853	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	1	15	theme	distinctive	212:222	arg1	roles					224:228	crucial and distinctive roles	200:228	crucial and distinctive roles	200:228	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	8	16	from	response	1687:1694	arg1	superiority					1600:1610	the unique superiority	1589:1610	the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response	1589:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	17	theme	terminal	129:136	arg1	end					138:140	the terminal end	125:140	the terminal end of glycans	125:151	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	9	18	theme	supramolecular	1746:1759	arg1	gels					1761:1764	supramolecular gels	1746:1764	supramolecular gels	1746:1764	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	8	19	theme	high-efficiency	1453:1467	arg1	acid					1476:1479	high-efficiency sialic acid	1453:1479	the high-efficiency sialic acid recognition effect	1449:1498	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	7	20	theme	bonding	1342:1348	arg1	interactions					1350:1361	hydrogen bonding interactions	1333:1361	hydrogen bonding interactions	1333:1361	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	5	21	theme	sialic	947:952	arg1	analogs					959:965	sialic acid analogs	947:965	sialic acid analogs	947:965	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	3	22	theme	PyHis	627:631	arg1	gel					649:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	5	23	theme	other	922:926	arg1	monosaccharides					928:942	other monosaccharides	922:942	other monosaccharides	922:942	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	8	24	theme	acid	1476:1479	arg1	effect					1493:1498	the high-efficiency sialic acid recognition effect	1449:1498	the high-efficiency sialic acid recognition effect	1449:1498	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	7	25	theme	sialic	1215:1220	arg1	acid					1222:1225	the sialic acid	1211:1225	the sialic acid	1211:1225	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	3	26	theme	histidine	616:624	arg1	gel					649:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	4	27	theme	intermolecular	693:706	arg1	bonds					717:721	intermolecular hydrogen bonds	693:721	intermolecular hydrogen bonds	693:721	Driven by π-π stacking and intermolecular hydrogen bonds, PyHis exhibits a strong self-assembly ability and forms stable gels.					
34076032	0	28	theme	acid	95:98	arg1	recognition					61:71	specific recognition	52:71	specific recognition of N-acetylneuraminic acid	52:98	Self-assembly gel-based dynamic response system for specific recognition of N-acetylneuraminic acid.					
34076032	5	29	theme	self-assembly	891:903	arg1	process					905:911	this self-assembly process	886:911	this self-assembly process	886:911	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	9	30	from	prospects	1727:1735	arg1	engineering					1778:1788	assembly engineering	1769:1788	assembly engineering	1769:1788	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	9	30	from	prospects	1727:1735	arg1	medicine					1804:1811	regenerative medicine	1791:1811	regenerative medicine	1791:1811	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	9	30	from	prospects	1727:1735	arg1	sorting					1826:1832	tumour cell sorting	1814:1832	tumour cell sorting	1814:1832	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	9	30	from	prospects	1727:1735	arg1	diagnosis					1845:1853	cancer diagnosis	1838:1853	cancer diagnosis	1838:1853	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	9	31	theme	regenerative	1791:1802	arg1	medicine					1804:1811	regenerative medicine	1791:1811	regenerative medicine	1791:1811	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	4	32	theme	strong	741:746	arg1	ability					762:768	a strong self-assembly ability	739:768	a strong self-assembly ability	739:768	Driven by π-π stacking and intermolecular hydrogen bonds, PyHis exhibits a strong self-assembly ability and forms stable gels.					
34076032	7	33	theme	mechanism	1188:1196	arg1	Analysis					1172:1179	Analysis	1172:1179	Analysis of the mechanism	1172:1196	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	2	34	theme	sialylated	510:519	arg1	glycans					521:527	sialylated glycans	510:527	sialylated glycans	510:527	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	4	35	theme	stable	780:785	arg1	gels					787:790	stable gels	780:790	stable gels	780:790	Driven by π-π stacking and intermolecular hydrogen bonds, PyHis exhibits a strong self-assembly ability and forms stable gels.					
34076032	2	36	theme	subtle	386:391	arg1	differences					404:414	the subtle structural differences	382:414	the subtle structural differences of sialic acid species	382:437	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	1	37	from	end	138:140	arg1	located					114:120	located	114:120	located	114:120	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	2	38	gly	sialylated	510:519	arg1	glycans					521:527	sialylated glycans	510:527	sialylated glycans	510:527	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	7	39	gly	sialylated	1230:1239	arg1	glycan					1241:1246	sialylated glycan	1230:1246	sialylated glycan	1230:1246	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	9	40	theme	cell	1821:1824	arg1	sorting					1826:1832	tumour cell sorting	1814:1832	tumour cell sorting	1814:1832	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	8	41	theme	single	1523:1528	arg1	level					1539:1543	the single molecule level	1519:1543	the single molecule level	1519:1543	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	42	theme	processes	277:285	arg1	development					303:313	neural development	296:313	neural development	296:313	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	42	theme	processes	277:285	arg1	cancers					357:363	cancers	357:363	cancers	357:363	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	42	theme	processes	277:285	arg1	processes					277:285	physiological and pathological processes	246:285	physiological and pathological processes	246:285	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	42	theme	processes	277:285	arg1	variety					235:241	a variety	233:241	a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers	233:363	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	42	theme	processes	277:285	arg1	interactions					326:337	cell-cell interactions	316:337	cell-cell interactions	316:337	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	1	42	theme	processes	277:285	arg1	autoimmunity					340:351	autoimmunity	340:351	autoimmunity	340:351	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	6	43	theme	remarkable	1047:1056	arg1	effect					1069:1074	a remarkable inhibitory effect	1045:1074	a remarkable inhibitory effect	1045:1074	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	2	44	theme	species	431:437	arg1	differences					404:414	the subtle structural differences	382:414	the subtle structural differences of sialic acid species	382:437	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	2	44	theme	species	431:437	arg1	composition					459:469	the complicated composition	443:469	the complicated composition of glycans	443:480	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	4	45	theme	self-assembly	748:760	arg1	ability					762:768	a strong self-assembly ability	739:768	a strong self-assembly ability	739:768	Driven by π-π stacking and intermolecular hydrogen bonds, PyHis exhibits a strong self-assembly ability and forms stable gels.					
34076032	2	46	theme	sialic	419:424	arg1	species					431:437	sialic acid species	419:437	sialic acid species	419:437	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	0	47	theme	dynamic	24:30	arg1	system					41:46	dynamic response system	24:46	dynamic response system for specific recognition of N-acetylneuraminic acid	24:98	Self-assembly gel-based dynamic response system for specific recognition of N-acetylneuraminic acid.					
34076032	7	48	theme	gelators	1415:1422	arg1	accumulation					1395:1406	the ordered accumulation	1383:1406	the ordered accumulation of the gelators	1383:1422	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	5	49	theme	acid	954:957	arg1	analogs					959:965	sialic acid analogs	947:965	sialic acid analogs	947:965	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	2	50	theme	glycans	474:480	arg1	differences					404:414	the subtle structural differences	382:414	the subtle structural differences of sialic acid species	382:437	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	2	50	theme	glycans	474:480	arg1	composition					459:469	the complicated composition	443:469	the complicated composition of glycans	443:480	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	1	51	theme	neural	296:301	arg1	development					303:313	neural development	296:313	neural development	296:313	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	7	52	theme	PyHis	1278:1282	arg1	molecules					1284:1292	two PyHis molecules	1274:1292	two PyHis molecules stacked together in the assemblies	1274:1327	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	8	53	theme	supramolecular	1556:1569	arg1	level					1571:1575	the supramolecular level	1552:1575	the supramolecular level	1552:1575	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	3	54	theme	supramolecular	634:647	arg1	gel					649:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	0	55	theme	specific	52:59	arg1	recognition					61:71	specific recognition	52:71	specific recognition of N-acetylneuraminic acid	52:98	Self-assembly gel-based dynamic response system for specific recognition of N-acetylneuraminic acid.					
34076032	6	56	gly	sialylated	1018:1027	arg1	glycan					1029:1034	a sialylated glycan	1016:1034	a sialylated glycan	1016:1034	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	5	57	theme	sialic	861:866	arg1	acid					868:871	a typical sialic acid	851:871	a typical sialic acid	851:871	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	5	57	theme	sialic	861:866	arg1	acid					845:848	N-acetylneuraminic acid	826:848	N-acetylneuraminic acid (a typical sialic acid)	826:872	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	6	58	theme	system	1164:1169	arg1	selectivity					1124:1134	the high selectivity	1115:1134	the high selectivity of the gel dynamic response system	1115:1169	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	8	59	theme	self-assembly	1634:1646	arg1	system					1648:1653	the supramolecular self-assembly system	1615:1653	the supramolecular self-assembly system in biomolecular recognition and response	1615:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	5	60	theme	typical	853:859	arg1	acid					868:871	a typical sialic acid	851:871	a typical sialic acid	851:871	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	5	60	theme	typical	853:859	arg1	acid					845:848	N-acetylneuraminic acid	826:848	N-acetylneuraminic acid (a typical sialic acid)	826:872	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	6	61	theme	dynamic	1147:1153	arg1	system					1164:1169	the gel dynamic response system	1139:1169	the gel dynamic response system	1139:1169	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	5	62	contain	have	967:970	arg2	effect					987:992	no significant effect	972:992	no significant effect	972:992	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	5	62	contain	have	967:970	arg1	monosaccharides					928:942	other monosaccharides	922:942	other monosaccharides	922:942	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	5	62	contain	have	967:970	arg1	analogs					959:965	sialic acid analogs	947:965	sialic acid analogs	947:965	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	3	63	theme	dynamic	563:569	arg1	system					580:585	a fluorescent dynamic response system	549:585	a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel	549:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	5	64	theme	N-acetylneuraminic	826:843	arg1	acid					868:871	a typical sialic acid	851:871	a typical sialic acid	851:871	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	5	64	theme	N-acetylneuraminic	826:843	arg1	acid					845:848	N-acetylneuraminic acid	826:848	N-acetylneuraminic acid (a typical sialic acid)	826:872	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	9	65	theme	assembly	1769:1776	arg1	engineering					1778:1788	assembly engineering	1769:1788	assembly engineering	1769:1788	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	9	66	theme	cancer	1838:1843	arg1	diagnosis					1845:1853	cancer diagnosis	1838:1853	cancer diagnosis	1838:1853	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	8	67	from	system	1648:1653	arg1	recognition					1671:1681	biomolecular recognition	1658:1681	biomolecular recognition	1658:1681	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	8	67	from	system	1648:1653	arg1	response					1687:1694	response	1687:1694	response	1687:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	8	68	theme	sialic	1469:1474	arg1	acid					1476:1479	high-efficiency sialic acid	1453:1479	the high-efficiency sialic acid recognition effect	1449:1498	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	4	69	theme	hydrogen	708:715	arg1	bonds					717:721	intermolecular hydrogen bonds	693:721	intermolecular hydrogen bonds	693:721	Driven by π-π stacking and intermolecular hydrogen bonds, PyHis exhibits a strong self-assembly ability and forms stable gels.					
34076032	6	70	theme	sialylated	1018:1027	arg1	glycan					1029:1034	a sialylated glycan	1016:1034	a sialylated glycan	1016:1034	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	8	71	theme	recognition	1481:1491	arg1	effect					1493:1498	the high-efficiency sialic acid recognition effect	1449:1498	the high-efficiency sialic acid recognition effect	1449:1498	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	0	72	theme	N-acetylneuraminic	76:93	arg1	acid					95:98	N-acetylneuraminic acid	76:98	N-acetylneuraminic acid	76:98	Self-assembly gel-based dynamic response system for specific recognition of N-acetylneuraminic acid.					
34076032	3	73	theme	pyrene-conjugated	598:614	arg1	gel					649:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	a pyrene-conjugated histidine (PyHis) supramolecular gel	596:651	Here, a fluorescent dynamic response system based on a pyrene-conjugated histidine (PyHis) supramolecular gel is proposed.					
34076032	1	74	theme	glycans	145:151	arg1	end					138:140	the terminal end	125:140	the terminal end of glycans	125:151	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	2	75	theme	precise	487:493	arg1	difficult					532:540	difficult	532:540	difficult	532:540	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	2	75	theme	precise	487:493	arg1	recognition					495:505	the precise recognition	483:505	the precise recognition of sialylated glycans	483:527	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	7	76	theme	hydrogen	1333:1340	arg1	interactions					1350:1361	hydrogen bonding interactions	1333:1361	hydrogen bonding interactions	1333:1361	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34076032	4	77	theme	π-π	676:678	arg1	stacking					680:687	π-π stacking	676:687	π-π stacking	676:687	Driven by π-π stacking and intermolecular hydrogen bonds, PyHis exhibits a strong self-assembly ability and forms stable gels.					
34076032	2	78	theme	glycans	521:527	arg1	difficult					532:540	difficult	532:540	difficult	532:540	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	2	78	theme	glycans	521:527	arg1	recognition					495:505	the precise recognition	483:505	the precise recognition of sialylated glycans	483:527	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	8	79	from	recognition	1671:1681	arg1	superiority					1600:1610	the unique superiority	1589:1610	the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response	1589:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	80	theme	crucial	200:206	arg1	roles					224:228	crucial and distinctive roles	200:228	crucial and distinctive roles	200:228	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	9	81	theme	tumour	1814:1819	arg1	sorting					1826:1832	tumour cell sorting	1814:1832	tumour cell sorting	1814:1832	This work shows the promising prospects of using supramolecular gels in assembly engineering, regenerative medicine, tumour cell sorting and cancer diagnosis.					
34076032	8	82	theme	molecule	1530:1537	arg1	level					1539:1543	the single molecule level	1519:1543	the single molecule level	1519:1543	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	83	theme	Sialic	101:106	arg1	acids					108:112	Sialic acids	101:112	Sialic acids located at the terminal end of glycans	101:151	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	8	84	located	observed	1507:1514	arg2	effect					1493:1498	the high-efficiency sialic acid recognition effect	1449:1498	the high-efficiency sialic acid recognition effect	1449:1498	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	8	84	located	observed	1507:1514	arg1	level					1539:1543	the single molecule level	1519:1543	the single molecule level	1519:1543	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	6	85	theme	inhibitory	1058:1067	arg1	effect					1069:1074	a remarkable inhibitory effect	1045:1074	a remarkable inhibitory effect	1045:1074	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	0	86	theme	response	32:39	arg1	system					41:46	dynamic response system	24:46	dynamic response system for specific recognition of N-acetylneuraminic acid	24:98	Self-assembly gel-based dynamic response system for specific recognition of N-acetylneuraminic acid.					
34076032	1	87	theme	located	114:120	arg1	acids					108:112	Sialic acids	101:112	Sialic acids located at the terminal end of glycans	101:151	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	2	88	theme	acid	426:429	arg1	species					431:437	sialic acid species	419:437	sialic acid species	419:437	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	5	89	dep	prevent	878:884	arg1	whereas					914:920	whereas	914:920	whereas	914:920	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	2	90	theme	structural	393:402	arg1	differences					404:414	the subtle structural differences	382:414	the subtle structural differences of sialic acid species	382:437	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	6	91	contain	has	1041:1043	arg1	glycan					1029:1034	a sialylated glycan	1016:1034	a sialylated glycan	1016:1034	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	6	91	contain	has	1041:1043	arg2	effect					1069:1074	a remarkable inhibitory effect	1045:1074	a remarkable inhibitory effect	1045:1074	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	6	92	theme	high	1119:1122	arg1	selectivity					1124:1134	the high selectivity	1115:1134	the high selectivity of the gel dynamic response system	1115:1169	Interestingly, a sialylated glycan also has a remarkable inhibitory effect on the gel formation, which highlights the high selectivity of the gel dynamic response system.					
34076032	2	93	theme	complicated	447:457	arg1	composition					459:469	the complicated composition	443:469	the complicated composition of glycans	443:480	However, due to the subtle structural differences of sialic acid species and the complicated composition of glycans, the precise recognition of sialylated glycans is difficult.					
34076032	8	94	theme	biomolecular	1658:1669	arg1	recognition					1671:1681	biomolecular recognition	1658:1681	biomolecular recognition	1658:1681	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	1	95	theme	cell-cell	316:324	arg1	interactions					326:337	cell-cell interactions	316:337	cell-cell interactions	316:337	Sialic acids located at the terminal end of glycans are densely attached to cell surfaces and play crucial and distinctive roles in a variety of physiological and pathological processes, such as neural development, cell-cell interactions, autoimmunity and cancers.					
34076032	8	96	theme	unique	1593:1598	arg1	superiority					1600:1610	the unique superiority	1589:1610	the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response	1589:1694	It is worth noting that the high-efficiency sialic acid recognition effect is not observed at the single molecule level but at the supramolecular level, indicating the unique superiority of the supramolecular self-assembly system in biomolecular recognition and response.					
34076032	5	97	theme	significant	975:985	arg1	effect					987:992	no significant effect	972:992	no significant effect	972:992	It is found that introduction of N-acetylneuraminic acid (a typical sialic acid) can prevent this self-assembly process, whereas other monosaccharides or sialic acid analogs have no significant effect on it.					
34076032	7	98	theme	sialylated	1230:1239	arg1	glycan					1241:1246	sialylated glycan	1230:1246	sialylated glycan	1230:1246	Analysis of the mechanism reveals that the sialic acid or sialylated glycan can interact closely with two PyHis molecules stacked together in the assemblies via hydrogen bonding interactions, thereby preventing the ordered accumulation of the gelators.					
34206199	2	0	theme	wall	440:443	arg1	behavior					445:452	wall behavior	440:452	wall behavior	440:452	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	2	1	theme	physiological	492:504	arg1	consequences					506:517	physiological consequences	492:517	physiological consequences	492:517	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	3	2	theme	hydration	846:854	arg1	degree					836:841	degree	836:841	degree	836:841	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	3	2	theme	hydration	846:854	arg1	potential					822:830	water potential	816:830	water potential	816:830	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	0	3	from	Wall	136:139	arg1	Consequences					70:81	the Consequences	66:81	the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall	66:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	2	4	from	changes	401:407	arg1	hydration					417:425	wall hydration	412:425	wall hydration	412:425	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	1	5	theme	lower	224:228	arg1	molecules					255:263	lower molecular weight spacing molecules	224:263	lower molecular weight spacing molecules known as plasticizers	224:285	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	3	6	theme	cell	645:648	arg1	walls					650:654	plant cell walls	639:654	plant cell walls	639:654	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	2	7	theme	many	544:547	arg1	plants					549:554	many plants	544:554	many plants	544:554	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	5	8	theme	crop	1414:1417	arg1	resilience					1419:1428	crop resilience	1414:1428	crop resilience	1414:1428	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	4	9	theme	polysaccharide	1130:1143	arg1	composition					1145:1155	polysaccharide composition	1130:1155	polysaccharide composition	1130:1155	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	1	10	theme	molecular	230:238	arg1	molecules					255:263	lower molecular weight spacing molecules	224:263	lower molecular weight spacing molecules known as plasticizers	224:285	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	0	11	theme	Consequences	70:81	arg1	Exploration					51:61	An Exploration	48:61	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.	0:140	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	3	12	theme	plant	639:643	arg1	walls					650:654	plant cell walls	639:654	plant cell walls	639:654	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	3	13	theme	cellulose	670:678	arg1	composites					680:689	bacterial cellulose composites	660:689	bacterial cellulose composites with other cell wall polysaccharides	660:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	3	14	theme	materials	865:873	arg1	hydration					846:854	hydration	846:854	hydration of these materials	846:873	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	1	15	theme	weight	240:245	arg1	molecules					255:263	lower molecular weight spacing molecules	224:263	lower molecular weight spacing molecules known as plasticizers	224:285	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	0	16	theme	Effects	93:99	arg1	Consequences					70:81	the Consequences	66:81	the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall	66:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	4	17	theme	fragments	1060:1068	arg1	swelling					1032:1039	swelling	1032:1039	swelling of plant cell wall fragments in suspension	1032:1082	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	0	18	theme	Direct	86:91	arg1	Effects					93:99	Direct Effects	86:99	Direct Effects of Water Deficit on the Plant Cell Wall	86:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	3	19	theme	bacterial	660:668	arg1	composites					680:689	bacterial cellulose composites	660:689	bacterial cellulose composites with other cell wall polysaccharides	660:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	2	20	theme	field	579:583	arg1	conditions					585:594	field conditions	579:594	field conditions	579:594	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	4	21	theme	cellulose	987:995	arg1	rehydration					962:972	rehydration	962:972	rehydration of bacterial cellulose and cellulose composites	962:1020	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	0	22	theme	Cell	6:9	arg1	Hydration					16:24	Plant Cell Wall Hydration and Plant Physiology	0:45	Hydration	16:24	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	1	23	theme	similar	335:341	arg1	effects					343:349	similar effects	335:349	similar effects	335:349	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	3	24	theme	wall	707:710	arg1	polysaccharides					712:726	other cell wall polysaccharides	696:726	other cell wall polysaccharides	696:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	5	25	theme	wall	1333:1336	arg1	hydration					1338:1346	wall hydration	1333:1346	wall hydration	1333:1346	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	5	26	from	hydration	1338:1346	arg1	consequences					1306:1317	the consequences	1302:1317	the consequences of changes in wall hydration	1302:1346	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	0	27	theme	Plant	0:4	arg1	Hydration					16:24	Plant Cell Wall Hydration and Plant Physiology	0:45	Hydration	16:24	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	1	28	theme	spacing	247:253	arg1	molecules					255:263	lower molecular weight spacing molecules	224:263	lower molecular weight spacing molecules known as plasticizers	224:285	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	3	29	used	used	733:736	arg2	composites					680:689	bacterial cellulose composites	660:689	bacterial cellulose composites with other cell wall polysaccharides	660:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	3	29	used	used	733:736	arg2	Osmotica					597:604	Osmotica	597:604	Osmotica large enough to be excluded from plant cell walls	597:654	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	4	30	theme	composites	1011:1020	arg1	rehydration					962:972	rehydration	962:972	rehydration of bacterial cellulose and cellulose composites	962:1020	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	1	31	theme	molecules	255:263	arg1	addition					212:219	the addition	208:219	the addition of lower molecular weight spacing molecules known as plasticizers	208:285	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	0	32	theme	Water	104:108	arg1	Deficit					110:116	Water Deficit	104:116	Water Deficit	104:116	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	3	33	with	Osmotica	597:604	arg1	polysaccharides					712:726	other cell wall polysaccharides	696:726	other cell wall polysaccharides	696:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	1	34	theme	synthetic	163:171	arg1	polymers					173:180	synthetic polymers	163:180	synthetic polymers	163:180	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	0	35	theme	Wall	11:14	arg1	Hydration					16:24	Plant Cell Wall Hydration and Plant Physiology	0:45	Hydration	16:24	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	3	36	theme	other	696:700	arg1	polysaccharides					712:726	other cell wall polysaccharides	696:726	other cell wall polysaccharides	696:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	5	37	theme	water	1276:1280	arg1	content					1282:1288	cell wall water content	1266:1288	cell wall water content	1266:1288	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	3	38	theme	large	606:610	arg1	Osmotica					597:604	Osmotica	597:604	Osmotica large enough to be excluded from plant cell walls	597:654	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	1	39	theme	polymers	173:180	arg1	extensibility					146:158	The extensibility	142:158	The extensibility of synthetic polymers	142:180	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	0	40	theme	Plant	30:34	arg1	Physiology					36:45	Plant Cell Wall Hydration and Plant Physiology	0:45	Physiology	36:45	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	3	41	theme	cell	702:705	arg1	polysaccharides					712:726	other cell wall polysaccharides	696:726	other cell wall polysaccharides	696:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	5	42	theme	cell	1266:1269	arg1	content					1282:1288	cell wall water content	1266:1288	cell wall water content	1266:1288	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	4	43	theme	cell	1050:1053	arg1	fragments					1060:1068	plant cell wall fragments	1044:1068	plant cell wall fragments	1044:1068	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	0	44	theme	Deficit	110:116	arg1	Effects					93:99	Direct Effects	86:99	Direct Effects of Water Deficit on the Plant Cell Wall	86:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	1	45	theme	plant	354:358	arg1	walls					365:369	plant cell walls	354:369	plant cell walls	354:369	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	5	46	from	consequences	1306:1317	arg1	hydration					1338:1346	wall hydration	1333:1346	wall hydration	1333:1346	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	5	47	theme	such	1387:1390	arg1	mechanisms					1392:1401	such mechanisms	1387:1401	such mechanisms	1387:1401	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	2	48	theme	wall	412:415	arg1	hydration					417:425	wall hydration	412:425	wall hydration	412:425	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	0	49	theme	Plant	125:129	arg1	Wall					136:139	the Plant Cell Wall	121:139	the Plant Cell Wall	121:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	1	50	theme	cell	360:363	arg1	walls					365:369	plant cell walls	354:369	plant cell walls	354:369	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	2	51	theme	water	522:526	arg1	potentials					528:537	water potentials	522:537	water potentials	522:537	Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions.					
34206199	0	52	from	Effects	93:99	arg1	Wall					136:139	the Plant Cell Wall	121:139	the Plant Cell Wall	121:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	3	53	theme	water	816:820	arg1	potential					822:830	water potential	816:830	water potential	816:830	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	5	54	from	changes	1322:1328	arg1	hydration					1338:1346	wall hydration	1333:1346	wall hydration	1333:1346	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	0	55	dep	Hydration	16:24	arg1	Exploration					51:61	An Exploration	48:61	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.	0:140	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	4	56	theme	plant	1044:1048	arg1	fragments					1060:1068	plant cell wall fragments	1044:1068	plant cell wall fragments	1044:1068	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	0	57	theme	Cell	131:134	arg1	Wall					136:139	the Plant Cell Wall	121:139	the Plant Cell Wall	121:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	5	58	theme	wall	1271:1274	arg1	content					1282:1288	cell wall water content	1266:1288	cell wall water content	1266:1288	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	4	59	from	swelling	1032:1039	arg1	suspension					1073:1082	suspension	1073:1082	suspension	1073:1082	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	3	60	with	composites	680:689	arg1	polysaccharides					712:726	other cell wall polysaccharides	696:726	other cell wall polysaccharides	696:726	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
34206199	5	61	theme	plant	1207:1211	arg1	responses					1227:1235	plant environmental responses	1207:1235	plant environmental responses	1207:1235	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	0	62	from	Consequences	70:81	arg1	Wall					136:139	the Plant Cell Wall	121:139	the Plant Cell Wall	121:139	Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall.					
34206199	4	63	theme	wall	1055:1058	arg1	fragments					1060:1068	plant cell wall fragments	1044:1068	plant cell wall fragments	1044:1068	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	5	64	theme	environmental	1213:1225	arg1	responses					1227:1235	plant environmental responses	1207:1235	plant environmental responses	1207:1235	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	4	65	theme	bacterial	977:985	arg1	cellulose					987:995	bacterial cellulose and cellulose composites	977:1020	cellulose	987:995	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	5	66	theme	changes	1322:1328	arg1	consequences					1306:1317	the consequences	1302:1317	the consequences of changes in wall hydration	1302:1346	Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.					
34206199	4	67	theme	cellulose	1001:1009	arg1	composites					1011:1020	bacterial cellulose and cellulose composites	977:1020	composites	1011:1020	Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition.					
34206199	1	68	contain	have	330:333	arg2	effects					343:349	similar effects	335:349	similar effects	335:349	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	1	68	contain	have	330:333	arg1	water					320:324	water	320:324	water	320:324	The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls.					
34206199	3	69	theme	water	753:757	arg1	content					759:765	their water content	747:765	their water content	747:765	Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition.					
32735925	7	0	theme	In-vitro	960:967	arg1	result					973:978	In-vitro ZOI result	960:978	In-vitro ZOI result	960:978	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	8	1	theme	GO-based	1286:1293	arg1	nanocomposites					1295:1308	GO-based nanocomposites	1286:1308	GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation	1286:1397	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	0	2	theme	antimicrobial	78:90	arg1	potential					92:100	antimicrobial potential	78:100	antimicrobial potential	78:100	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	1	3	theme	ethylene	349:356	arg1	acid					378:381	ethylene diamine tetraacetic acid	349:381	ethylene diamine tetraacetic acid (EDTA)	349:388	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	1	3	theme	ethylene	349:356	arg1	EDTA					384:387	EDTA	384:387	EDTA	384:387	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	1	4	theme	synergistic	170:180	arg1	potential					196:204	the synergistic antimicrobial potential	166:204	the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO)	166:259	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	1	5	theme	alternative	265:275	arg1	approach					277:284	an alternative approach	262:284	an alternative approach	262:284	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	7	6	theme	Candida	1158:1164	arg1	22.0 mm					1176:1182	22.0 mm	1176:1182	22.0 mm	1176:1182	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	7	6	theme	Candida	1158:1164	arg1	albicans					1166:1173	Candida albicans	1158:1173	Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS)	1158:1217	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	7	6	theme	Candida	1158:1164	arg1	16.0 mm					1200:1206	16.0 mm	1200:1206	16.0 mm	1200:1206	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	1	7	theme	diamine	358:364	arg1	acid					378:381	ethylene diamine tetraacetic acid	349:381	ethylene diamine tetraacetic acid (EDTA)	349:388	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	1	7	theme	diamine	358:364	arg1	EDTA					384:387	EDTA	384:387	EDTA	384:387	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	3	8	theme	antimicrobial	519:531	arg1	activity					533:540	The antimicrobial activity	515:540	The antimicrobial activity of GO, GO-CS, and GO-EDTA	515:566	The antimicrobial activity of GO, GO-CS, and GO-EDTA was investigated against some pathogenic bacteria and Candida sp.					
32735925	2	9	theme	Raman	495:499	arg1	spectroscopy					501:512	Raman spectroscopy	495:512	Raman spectroscopy	495:512	The synthesized GO-nanocomposites were identified by XRD, HRTEM, SEM, FTIR, Zeta potential, and Raman spectroscopy.					
32735925	1	10	theme	tetraacetic	366:376	arg1	acid					378:381	ethylene diamine tetraacetic acid	349:381	ethylene diamine tetraacetic acid (EDTA)	349:388	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	1	10	theme	tetraacetic	366:376	arg1	EDTA					384:387	EDTA	384:387	EDTA	384:387	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	9	11	theme	eco-friendly	1552:1563	arg1	approach					1565:1572	a cost-effective and eco-friendly approach	1531:1572	a cost-effective and eco-friendly approach	1531:1572	This work provides revolutionary GO-nanocomposites for increasing the antimicrobial activity against some pathogenic microbes with a cost-effective and eco-friendly approach.					
32735925	9	12	theme	pathogenic	1506:1515	arg1	microbes					1517:1524	some pathogenic microbes	1501:1524	some pathogenic microbes	1501:1524	This work provides revolutionary GO-nanocomposites for increasing the antimicrobial activity against some pathogenic microbes with a cost-effective and eco-friendly approach.					
32735925	1	13	theme	acid	378:381	arg1	hybridization					315:327	the hybridization	311:327	the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO	311:396	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	8	14	theme	different	1317:1325	arg1	stress					1369:1374	oxidative and membrane stress	1346:1374	stress	1369:1374	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	8	14	theme	different	1317:1325	arg1	isolation					1389:1397	wrapping isolation	1380:1397	wrapping isolation	1380:1397	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	8	14	theme	different	1317:1325	arg1	mechanisms					1327:1336	different mechanisms	1317:1336	different mechanisms such as oxidative and membrane stress and wrapping isolation	1317:1397	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	1	15	theme	antimicrobial	182:194	arg1	potential					196:204	the synergistic antimicrobial potential	166:204	the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO)	166:259	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	0	16	theme	Candida	115:121	arg1	species					123:129	some Candida species	110:129	some Candida species	110:129	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	17	theme	oxide-based	9:19	arg1	nanocomposites					21:34	Graphene oxide-based nanocomposites	0:34	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.	0:154	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	17	theme	oxide-based	9:19	arg1	GO-EDTA					53:59	GO-EDTA	53:59	GO-EDTA	53:59	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	17	theme	oxide-based	9:19	arg1	GO-chitosan					37:47	GO-chitosan	37:47	GO-chitosan	37:47	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	8	18	theme	wrapping	1380:1387	arg1	isolation					1389:1397	wrapping isolation	1380:1397	wrapping isolation	1380:1397	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	1	19	with	hybridization	315:327	arg1	GO					395:396	GO	395:396	GO	395:396	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	9	20	theme	revolutionary	1419:1431	arg1	GO-nanocomposites					1433:1449	revolutionary GO-nanocomposites	1419:1449	revolutionary GO-nanocomposites	1419:1449	This work provides revolutionary GO-nanocomposites for increasing the antimicrobial activity against some pathogenic microbes with a cost-effective and eco-friendly approach.					
32735925	0	21	theme	Graphene	0:7	arg1	nanocomposites					21:34	Graphene oxide-based nanocomposites	0:34	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.	0:154	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	21	theme	Graphene	0:7	arg1	GO-EDTA					53:59	GO-EDTA	53:59	GO-EDTA	53:59	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	21	theme	Graphene	0:7	arg1	GO-chitosan					37:47	GO-chitosan	37:47	GO-chitosan	37:47	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	3	22	theme	GO-CS	549:553	arg1	activity					533:540	The antimicrobial activity	515:540	The antimicrobial activity of GO, GO-CS, and GO-EDTA	515:566	The antimicrobial activity of GO, GO-CS, and GO-EDTA was investigated against some pathogenic bacteria and Candida sp.					
32735925	4	23	theme	exterior	731:738	arg1	part					740:743	the exterior part	727:743	the exterior part	727:743	Results showed that nano-composites looked flattened and clear, with some lines and folds on the exterior part.					
32735925	1	24	theme	nano-composites	209:223	arg1	potential					196:204	the synergistic antimicrobial potential	166:204	the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO)	166:259	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	5	25	theme	remarkable	800:809	arg1	holes					811:815	remarkable holes	800:815	remarkable holes	800:815	SEM images show the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure.					
32735925	6	26	theme	pathogenic	939:948	arg1	microbes					950:957	all pathogenic microbes	935:957	all pathogenic microbes	935:957	GO-EDTA and GO-CS possess a promising antimicrobial activity against all pathogenic microbes.					
32735925	0	27	theme	pathogenic	135:144	arg1	bacteria					146:153	pathogenic bacteria	135:153	pathogenic bacteria	135:153	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	8	28	theme	oxidative	1346:1354	arg1	stress					1369:1374	oxidative and membrane stress	1346:1374	stress	1369:1374	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	8	29	theme	membrane	1360:1367	arg1	stress					1369:1374	oxidative and membrane stress	1346:1374	stress	1369:1374	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	5	30	theme	GO	786:787	arg1	morphology					772:781	the basic morphology	762:781	the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure	762:863	SEM images show the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure.					
32735925	3	31	theme	Candida	622:628	arg1	sp					630:631	Candida sp	622:631	Candida sp	622:631	The antimicrobial activity of GO, GO-CS, and GO-EDTA was investigated against some pathogenic bacteria and Candida sp.					
32735925	3	32	theme	GO	545:546	arg1	activity					533:540	The antimicrobial activity	515:540	The antimicrobial activity of GO, GO-CS, and GO-EDTA	515:566	The antimicrobial activity of GO, GO-CS, and GO-EDTA was investigated against some pathogenic bacteria and Candida sp.					
32735925	9	33	theme	cost-effective	1533:1546	arg1	approach					1565:1572	a cost-effective and eco-friendly approach	1531:1572	a cost-effective and eco-friendly approach	1531:1572	This work provides revolutionary GO-nanocomposites for increasing the antimicrobial activity against some pathogenic microbes with a cost-effective and eco-friendly approach.					
32735925	5	34	theme	SEM	746:748	arg1	images					750:755	SEM images	746:755	SEM images	746:755	SEM images show the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure.					
32735925	1	35	theme	graphene	241:248	arg1	oxide					250:254	graphene oxide	241:254	graphene oxide (GO)	241:259	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	3	36	theme	GO-EDTA	560:566	arg1	activity					533:540	The antimicrobial activity	515:540	The antimicrobial activity of GO, GO-CS, and GO-EDTA	515:566	The antimicrobial activity of GO, GO-CS, and GO-EDTA was investigated against some pathogenic bacteria and Candida sp.					
32735925	6	37	contain	possess	884:890	arg1	GO-CS					878:882	GO-CS	878:882	GO-CS	878:882	GO-EDTA and GO-CS possess a promising antimicrobial activity against all pathogenic microbes.					
32735925	6	37	contain	possess	884:890	arg2	activity					918:925	a promising antimicrobial activity	892:925	a promising antimicrobial activity against all pathogenic microbes	892:957	GO-EDTA and GO-CS possess a promising antimicrobial activity against all pathogenic microbes.					
32735925	6	37	contain	possess	884:890	arg1	GO-EDTA					866:872	GO-EDTA	866:872	GO-EDTA	866:872	GO-EDTA and GO-CS possess a promising antimicrobial activity against all pathogenic microbes.					
32735925	2	38	theme	synthesized	403:413	arg1	GO-nanocomposites					415:431	The synthesized GO-nanocomposites	399:431	The synthesized GO-nanocomposites	399:431	The synthesized GO-nanocomposites were identified by XRD, HRTEM, SEM, FTIR, Zeta potential, and Raman spectroscopy.					
32735925	3	39	theme	pathogenic	598:607	arg1	bacteria					609:616	some pathogenic bacteria	593:616	some pathogenic bacteria	593:616	The antimicrobial activity of GO, GO-CS, and GO-EDTA was investigated against some pathogenic bacteria and Candida sp.					
32735925	7	40	dep	coli	1038:1041	arg1	22.0 mm					1044:1050	22.0 mm	1044:1050	22.0 mm	1044:1050	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	7	40	dep	coli	1038:1041	arg1	11.0 mm					1068:1074	11.0 mm	1068:1074	11.0 mm	1068:1074	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	1	41	theme	chitosan	332:339	arg1	hybridization					315:327	the hybridization	311:327	the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO	311:396	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	8	42	theme	due	1310:1312	arg1	nanocomposites					1295:1308	GO-based nanocomposites	1286:1308	GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation	1286:1397	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	7	43	dep	aureus	1103:1108	arg1	10.0 mm					1135:1141	10.0 mm	1135:1141	10.0 mm	1135:1141	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	7	43	dep	aureus	1103:1108	arg1	15.0 mm					1111:1117	15.0 mm	1111:1117	15.0 mm	1111:1117	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	6	44	theme	promising	894:902	arg1	activity					918:925	a promising antimicrobial activity	892:925	a promising antimicrobial activity against all pathogenic microbes	892:957	GO-EDTA and GO-CS possess a promising antimicrobial activity against all pathogenic microbes.					
32735925	7	45	dep	albicans	1166:1173	arg1	22.0 mm					1176:1182	22.0 mm	1176:1182	22.0 mm	1176:1182	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	7	45	dep	albicans	1166:1173	arg1	albicans					1166:1173	Candida albicans	1158:1173	Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS)	1158:1217	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	7	45	dep	albicans	1166:1173	arg1	16.0 mm					1200:1206	16.0 mm	1200:1206	16.0 mm	1200:1206	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	0	46	dep	nanocomposites	21:34	arg1	nanocomposites					21:34	Graphene oxide-based nanocomposites	0:34	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.	0:154	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	46	dep	nanocomposites	21:34	arg1	GO-EDTA					53:59	GO-EDTA	53:59	GO-EDTA	53:59	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	0	46	dep	nanocomposites	21:34	arg1	GO-chitosan					37:47	GO-chitosan	37:47	GO-chitosan	37:47	Graphene oxide-based nanocomposites (GO-chitosan and GO-EDTA) for outstanding antimicrobial potential against some Candida species and pathogenic bacteria.					
32735925	7	47	theme	ZOI	969:971	arg1	result					973:978	In-vitro ZOI result	960:978	In-vitro ZOI result	960:978	In-vitro ZOI result verified that they exhibited activity against Escherichia coli (22.0 mm for GO-EDTA and 11.0 mm for GO-CS), Staphylococcus aureus (15.0 mm for GO-EDTA and 10.0 mm for GO-CS) and Candida albicans (22.0 mm for GO-EDTA and 16.0 mm for GO-CS).					
32735925	5	48	theme	internal	846:853	arg1	structure					855:863	indeclinable internal structure	833:863	indeclinable internal structure	833:863	SEM images show the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure.					
32735925	9	49	theme	antimicrobial	1470:1482	arg1	activity					1484:1491	the antimicrobial activity	1466:1491	the antimicrobial activity against some pathogenic microbes	1466:1524	This work provides revolutionary GO-nanocomposites for increasing the antimicrobial activity against some pathogenic microbes with a cost-effective and eco-friendly approach.					
32735925	8	50	theme	Microbial	1220:1228	arg1	cells					1230:1234	Microbial cells	1220:1234	Microbial cells	1220:1234	Microbial cells may be ultimately-damaged when they interact with GO-based nanocomposites due to different mechanisms such as oxidative and membrane stress and wrapping isolation.					
32735925	5	51	theme	indeclinable	833:844	arg1	structure					855:863	indeclinable internal structure	833:863	indeclinable internal structure	833:863	SEM images show the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure.					
32735925	2	52	theme	Zeta	475:478	arg1	potential					480:488	Zeta potential	475:488	Zeta potential	475:488	The synthesized GO-nanocomposites were identified by XRD, HRTEM, SEM, FTIR, Zeta potential, and Raman spectroscopy.					
32735925	1	53	dep	oxide	250:254	arg1	GO					257:258	GO	257:258	GO	257:258	To obtain the synergistic antimicrobial potential of nano-composites conjugated with graphene oxide (GO), an alternative approach was developed throughout the hybridization of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) with GO.					
32735925	5	54	theme	basic	766:770	arg1	morphology					772:781	the basic morphology	762:781	the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure	762:863	SEM images show the basic morphology of GO which owns remarkable holes, crevasses, and indeclinable internal structure.					
32735925	6	55	theme	antimicrobial	904:916	arg1	activity					918:925	a promising antimicrobial activity	892:925	a promising antimicrobial activity against all pathogenic microbes	892:957	GO-EDTA and GO-CS possess a promising antimicrobial activity against all pathogenic microbes.					
34798188	0	0	theme	aminated	75:82	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	1	1	theme	methyl	316:321	arg1	orange					323:328	methyl orange	316:328	methyl orange	316:328	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	2	2	theme	prepared	335:342	arg1	aerogel					360:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel was well characterized via SEM, EDX, FT-IR, XRD and XPS to reveal the microstructure and composition.					
34798188	1	3	theme	oxide	243:247	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	3	theme	oxide	243:247	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	0	4	theme	@	74:74	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	5	5	theme	pore	840:843	arg1	size					845:848	the pore size	836:848	the pore size	836:848	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	9	6	from	time	1437:1440	arg1	forms					1419:1423	different forms	1409:1423	different forms at the same time	1409:1440	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	6	7	theme	pore	921:924	arg1	moderate					934:941	moderate	934:941	moderate	934:941	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	6	7	theme	pore	921:924	arg1	size					926:929	the pore size	917:929	the pore size	917:929	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	0	8	theme	oxide	93:97	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	6	9	theme	methyl	1063:1068	arg1	orange					1070:1075	methyl orange	1063:1075	methyl orange (412 mg/g)	1063:1086	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	6	9	theme	methyl	1063:1068	arg1	412 mg/g					1078:1085	412 mg/g	1078:1085	412 mg/g	1078:1085	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	5	10	theme	@	894:894	arg1	aerogel					903:909	MOF-199@AFGO/CS aerogel	887:909	MOF-199@AFGO/CS aerogel	887:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	0	11	theme	graphene	84:91	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	9	12	theme	different	1409:1417	arg1	forms					1419:1423	different forms	1409:1423	different forms at the same time	1409:1440	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	7	13	theme	adsorption	1128:1137	arg1	process					1139:1145	the adsorption process	1124:1145	the adsorption process at equilibrium	1124:1160	Furthermore, the adsorption process at equilibrium followed the Freundlich isotherm model.					
34798188	5	14	theme	AFGO/CS	895:901	arg1	aerogel					903:909	MOF-199@AFGO/CS aerogel	887:909	MOF-199@AFGO/CS aerogel	887:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	1	15	theme	MOF-199	258:264	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	15	theme	MOF-199	258:264	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	5	16	theme	aerogel	903:909	arg1	aerogel					903:909	MOF-199@AFGO/CS aerogel	887:909	MOF-199@AFGO/CS aerogel	887:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	16	theme	aerogel	903:909	arg1	groups					877:882	the surface functional groups	854:882	the surface functional groups of MOF-199@AFGO/CS aerogel	854:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	16	theme	aerogel	903:909	arg1	size					845:848	the pore size	836:848	the pore size	836:848	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	17	theme	aerogel	813:819	arg1	related					825:831	related	825:831	related	825:831	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	17	theme	aerogel	813:819	arg1	capacity					785:792	the adsorption capacity	770:792	the adsorption capacity of MOF-199@AFGO/CS aerogel	770:819	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	18	theme	@	804:804	arg1	aerogel					813:819	MOF-199@AFGO/CS aerogel	797:819	MOF-199@AFGO/CS aerogel	797:819	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	19	theme	methyl	744:749	arg1	orange					751:756	methyl orange	744:756	methyl orange	744:756	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	20	theme	formaldehyde	723:734	arg1	gas					736:738	formaldehyde gas	723:738	formaldehyde gas	723:738	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	7	21	from	equilibrium	1150:1160	arg1	process					1139:1145	the adsorption process	1124:1145	the adsorption process at equilibrium	1124:1160	Furthermore, the adsorption process at equilibrium followed the Freundlich isotherm model.					
34798188	3	22	theme	mechanical	483:492	arg1	property					494:501	the mechanical property and the stability	479:519	property	494:501	Besides, the mechanical property and the stability of MOF-199@AFGO/CS aerogel were investigated.					
34798188	4	23	theme	AFGO/CS	599:605	arg1	aerogel					607:613	MOF-199@AFGO/CS aerogel	591:613	MOF-199@AFGO/CS aerogel	591:613	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	7	24	theme	isotherm	1186:1193	arg1	model					1195:1199	the Freundlich isotherm model	1171:1199	the Freundlich isotherm model	1171:1199	Furthermore, the adsorption process at equilibrium followed the Freundlich isotherm model.					
34798188	5	25	theme	functional	866:875	arg1	aerogel					903:909	MOF-199@AFGO/CS aerogel	887:909	MOF-199@AFGO/CS aerogel	887:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	25	theme	functional	866:875	arg1	groups					877:882	the surface functional groups	854:882	the surface functional groups of MOF-199@AFGO/CS aerogel	854:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	4	26	theme	good	619:622	arg1	stability					624:632	good stability	619:632	good stability	619:632	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	1	27	theme	@	265:265	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	27	theme	@	265:265	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	8	28	theme	pseudo-second-order	1246:1264	arg1	model					1266:1270	the pseudo-second-order model	1242:1270	the pseudo-second-order model	1242:1270	The kinetic behavior was well fitted by the pseudo-second-order model, indicating chemisorption as the rate-determining step.					
34798188	2	29	theme	AFGO/CS	352:358	arg1	aerogel					360:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel was well characterized via SEM, EDX, FT-IR, XRD and XPS to reveal the microstructure and composition.					
34798188	6	30	from	group	957:961	arg1	aerogel					982:988	the aerogel	978:988	the aerogel	978:988	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	1	31	theme	metal-organic	191:203	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	31	theme	metal-organic	191:203	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	0	32	theme	chitosan	15:22	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan	0:22	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	6	33	theme	orange	1070:1075	arg1	capacity					1016:1023	the adsorption capacity	1001:1023	the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g)	1001:1086	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	5	34	from	study	692:696	arg1	ability					705:711	the ability to adsorb formaldehyde gas and methyl orange	701:756	the ability to adsorb formaldehyde gas and methyl orange	701:756	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	35	theme	MOF-199	887:893	arg1	aerogel					903:909	MOF-199@AFGO/CS aerogel	887:909	MOF-199@AFGO/CS aerogel	887:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	9	36	theme	same	1432:1435	arg1	time					1437:1440	the same time	1428:1440	the same time	1428:1440	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	1	37	theme	framework	205:213	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	37	theme	framework	205:213	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	0	38	theme	metal-organic	41:53	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	6	39	theme	formaldehyde	1028:1039	arg1	197.89 mg/g					1046:1056	197.89 mg/g	1046:1056	197.89 mg/g	1046:1056	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	6	39	theme	formaldehyde	1028:1039	arg1	gas					1041:1043	formaldehyde gas	1028:1043	formaldehyde gas (197.89 mg/g)	1028:1057	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	0	40	theme	formaldehyde	129:140	arg1	gas					142:144	formaldehyde gas	129:144	formaldehyde gas	129:144	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	6	41	theme	amino	951:955	arg1	group					957:961	the amino group	947:961	the amino group	947:961	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	3	42	theme	aerogel	540:546	arg1	property					494:501	the mechanical property and the stability	479:519	property	494:501	Besides, the mechanical property and the stability of MOF-199@AFGO/CS aerogel were investigated.					
34798188	3	42	theme	aerogel	540:546	arg1	stability					511:519	the mechanical property and the stability	479:519	stability	511:519	Besides, the mechanical property and the stability of MOF-199@AFGO/CS aerogel were investigated.					
34798188	4	43	theme	@	598:598	arg1	aerogel					607:613	MOF-199@AFGO/CS aerogel	591:613	MOF-199@AFGO/CS aerogel	591:613	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	8	44	theme	rate-determining	1305:1320	arg1	step					1322:1325	the rate-determining step	1301:1325	the rate-determining step	1301:1325	The kinetic behavior was well fitted by the pseudo-second-order model, indicating chemisorption as the rate-determining step.					
34798188	8	44	theme	rate-determining	1305:1320	arg1	chemisorption					1284:1296	chemisorption	1284:1296	chemisorption	1284:1296	The kinetic behavior was well fitted by the pseudo-second-order model, indicating chemisorption as the rate-determining step.					
34798188	8	45	theme	kinetic	1206:1212	arg1	behavior					1214:1221	The kinetic behavior	1202:1221	The kinetic behavior	1202:1221	The kinetic behavior was well fitted by the pseudo-second-order model, indicating chemisorption as the rate-determining step.					
34798188	9	46	theme	liquid	1501:1506	arg1	pollutants					1508:1517	liquid pollutants	1501:1517	liquid pollutants	1501:1517	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	5	47	theme	MOF-199	797:803	arg1	aerogel					813:819	MOF-199@AFGO/CS aerogel	797:819	MOF-199@AFGO/CS aerogel	797:819	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	1	48	theme	MOF-199	216:222	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	48	theme	MOF-199	216:222	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	9	49	theme	potential	1451:1459	arg1	application					1461:1471	potential application	1451:1471	potential application	1451:1471	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	3	50	theme	@	531:531	arg1	aerogel					540:546	MOF-199@AFGO/CS aerogel	524:546	MOF-199@AFGO/CS aerogel	524:546	Besides, the mechanical property and the stability of MOF-199@AFGO/CS aerogel were investigated.					
34798188	6	51	theme	adsorption	1005:1014	arg1	capacity					1016:1023	the adsorption capacity	1001:1023	the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g)	1001:1086	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	6	52	theme	gas	1041:1043	arg1	capacity					1016:1023	the adsorption capacity	1001:1023	the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g)	1001:1086	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	0	53	theme	framework	55:63	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	0	54	theme	gas	142:144	arg1	adsorption					115:124	the adsorption	111:124	the adsorption of formaldehyde gas and methyl orange	111:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	4	55	contain	had	615:617	arg1	aerogel					607:613	MOF-199@AFGO/CS aerogel	591:613	MOF-199@AFGO/CS aerogel	591:613	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	4	55	contain	had	615:617	arg2	stability					624:632	good stability	619:632	good stability	619:632	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	3	56	theme	AFGO/CS	532:538	arg1	aerogel					540:546	MOF-199@AFGO/CS aerogel	524:546	MOF-199@AFGO/CS aerogel	524:546	Besides, the mechanical property and the stability of MOF-199@AFGO/CS aerogel were investigated.					
34798188	4	57	theme	compression	644:654	arg1	resilience					656:665	compression resilience	644:665	compression resilience	644:665	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	0	58	theme	orange	157:162	arg1	adsorption					115:124	the adsorption	111:124	the adsorption of formaldehyde gas and methyl orange	111:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	9	59	dep	adsorbent	1375:1383	arg1	remove					1388:1393	remove	1388:1393	to remove pollutants in different forms at the same time	1385:1440	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	9	60	theme	gas	1523:1525	arg1	pollutants					1527:1536	gas pollutants	1523:1536	gas pollutants	1523:1536	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	6	61	from	MOF-199	967:973	arg1	aerogel					982:988	the aerogel	978:988	the aerogel	978:988	When the pore size is moderate, as the amino group and MOF-199 on the aerogel increased, the adsorption capacity of formaldehyde gas (197.89 mg/g) and methyl orange (412 mg/g) can reach the maximum.					
34798188	0	62	theme	methyl	150:155	arg1	orange					157:162	methyl orange	150:162	methyl orange	150:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	5	63	theme	surface	858:864	arg1	aerogel					903:909	MOF-199@AFGO/CS aerogel	887:909	MOF-199@AFGO/CS aerogel	887:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	63	theme	surface	858:864	arg1	groups					877:882	the surface functional groups	854:882	the surface functional groups of MOF-199@AFGO/CS aerogel	854:909	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	1	64	theme	@	224:224	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	64	theme	@	224:224	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	9	65	contain	has	1447:1449	arg2	application					1461:1471	potential application	1451:1471	potential application	1451:1471	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	9	65	contain	has	1447:1449	arg1	work					1333:1336	This work	1328:1336	This work	1328:1336	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	4	66	theme	MOF-199	591:597	arg1	aerogel					607:613	MOF-199@AFGO/CS aerogel	591:613	MOF-199@AFGO/CS aerogel	591:613	The results showed that MOF-199@AFGO/CS aerogel had good stability in water, compression resilience and thermostability.					
34798188	5	67	theme	adsorption	774:783	arg1	related					825:831	related	825:831	related	825:831	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	5	67	theme	adsorption	774:783	arg1	capacity					785:792	the adsorption capacity	770:792	the adsorption capacity of MOF-199@AFGO/CS aerogel	770:819	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	2	68	theme	@	351:351	arg1	aerogel					360:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel was well characterized via SEM, EDX, FT-IR, XRD and XPS to reveal the microstructure and composition.					
34798188	5	69	theme	AFGO/CS	805:811	arg1	aerogel					813:819	MOF-199@AFGO/CS aerogel	797:819	MOF-199@AFGO/CS aerogel	797:819	The study on the ability to adsorb formaldehyde gas and methyl orange showed that the adsorption capacity of MOF-199@AFGO/CS aerogel was related to the pore size and the surface functional groups of MOF-199@AFGO/CS aerogel.					
34798188	1	70	theme	aminated	225:232	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	70	theme	aminated	225:232	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	0	71	theme	MOF-199	66:72	arg1	aerogel					99:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	41:105	metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange	41:162	Preparation of chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel for the adsorption of formaldehyde gas and methyl orange.					
34798188	9	72	theme	reliable	1352:1359	arg1	basis					1361:1365	a reliable basis	1350:1365	a reliable basis for the adsorbent to remove pollutants in different forms at the same time	1350:1440	This work can provide a reliable basis for the adsorbent to remove pollutants in different forms at the same time, and has potential application in simultaneously adsorbing liquid pollutants and gas pollutants.					
34798188	7	73	theme	Freundlich	1175:1184	arg1	model					1195:1199	the Freundlich isotherm model	1171:1199	the Freundlich isotherm model	1171:1199	Furthermore, the adsorption process at equilibrium followed the Freundlich isotherm model.					
34798188	2	74	theme	MOF-199	344:350	arg1	aerogel					360:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel	331:366	The prepared MOF-199@AFGO/CS aerogel was well characterized via SEM, EDX, FT-IR, XRD and XPS to reveal the microstructure and composition.					
34798188	1	75	theme	graphene	234:241	arg1	aerogel					249:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel	191:255	metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS)	191:273	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
34798188	1	75	theme	graphene	234:241	arg1	AFGO/CS					266:272	MOF-199@AFGO/CS	258:272	MOF-199@AFGO/CS	258:272	Chitosan crosslinked with metal-organic framework (MOF-199)@aminated graphene oxide aerogel (MOF-199@AFGO/CS) were prepared to adsorb formaldehyde and methyl orange.					
32456931	0	0	theme	near-infrared	70:82	arg1	means					152:156	a means	150:156	a means to provide pure profiles of milk and of both adulterants with forensic evidence	150:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	0	0	theme	near-infrared	70:82	arg1	spectroscopy					84:95	near-infrared spectroscopy	70:95	near-infrared spectroscopy with DD-SIMCA	70:109	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	1	1	theme	present	266:272	arg1	communication					280:292	The present short communication	262:292	The present short communication	262:292	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	0	2	theme	pure	169:172	arg1	profiles					174:181	pure profiles	169:181	pure profiles of milk and of both adulterants with forensic evidence	169:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	5	3	theme	bilinear	1313:1320	arg1	decomposition					1327:1339	successful bilinear data decomposition	1302:1339	successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants)	1302:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	4	4	theme	quantification	1241:1254	arg1	analysis					1256:1263	the quantification analysis	1237:1263	the quantification analysis	1237:1263	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	3	5	theme	complementary	842:854	arg1	model					868:872	a complementary chemometric model	840:872	a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles	840:918	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	5	theme	complementary	842:854	arg1	least-squares					801:813	Multivariate curve resolution - alternating least-squares	757:813	Multivariate curve resolution - alternating least-squares (MCR-ALS)	757:823	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	6	theme	Multivariate	757:768	arg1	model					868:872	a complementary chemometric model	840:872	a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles	840:918	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	6	theme	Multivariate	757:768	arg1	MCR-ALS					816:822	MCR-ALS	816:822	MCR-ALS	816:822	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	6	theme	Multivariate	757:768	arg1	least-squares					801:813	Multivariate curve resolution - alternating least-squares	757:813	Multivariate curve resolution - alternating least-squares (MCR-ALS)	757:823	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	0	7	with	adulterants	203:213	arg1	evidence					229:236	forensic evidence	220:236	forensic evidence	220:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	2	8	theme	powder	582:587	arg1	samples					558:564	all samples	554:564	all samples of genuine milk powder	554:587	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	2	8	theme	powder	582:587	arg1	members					592:598	members	592:598	members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	592:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	3	9	theme	curve	770:774	arg1	model					868:872	a complementary chemometric model	840:872	a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles	840:918	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	9	theme	curve	770:774	arg1	MCR-ALS					816:822	MCR-ALS	816:822	MCR-ALS	816:822	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	9	theme	curve	770:774	arg1	least-squares					801:813	Multivariate curve resolution - alternating least-squares	757:813	Multivariate curve resolution - alternating least-squares (MCR-ALS)	757:823	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	5	10	theme	strong	1498:1503	arg1	profiles					1517:1524	strong overlapping profiles	1498:1524	strong overlapping profiles	1498:1524	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	0	11	from	Detection	0:8	arg1	powder					57:62	milk powder	52:62	milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence	52:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	2	12	theme	milk	577:580	arg1	powder					582:587	genuine milk powder	569:587	genuine milk powder	569:587	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	0	13	theme	adulterants	203:213	arg1	profiles					174:181	pure profiles	169:181	pure profiles of milk and of both adulterants with forensic evidence	169:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	3	14	dep	attributed	987:996	arg1	not					1021:1023	not	1021:1023	not	1021:1023	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	14	dep	attributed	987:996	arg1	samples					970:976	samples	970:976	samples properly attributed in the target class or not, providing further investigation from forensic point of view	970:1084	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	14	dep	attributed	987:996	arg1	class					1012:1016	the target class	1001:1016	the target class	1001:1016	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	2	15	theme	one-class	502:510	arg1	classifier					512:521	This one-class classifier	497:521	This one-class classifier	497:521	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	2	16	theme	genuine	569:575	arg1	powder					582:587	genuine milk powder	569:587	genuine milk powder	569:587	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	3	17	theme	chemical	946:953	arg1	composition					955:965	the chemical composition	942:965	the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view	942:1084	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	18	theme	chemometric	856:866	arg1	model					868:872	a complementary chemometric model	840:872	a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles	840:918	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	18	theme	chemometric	856:866	arg1	least-squares					801:813	Multivariate curve resolution - alternating least-squares	757:813	Multivariate curve resolution - alternating least-squares (MCR-ALS)	757:823	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	2	19	theme	milk	636:639	arg1	powder					641:646	milk powder	636:646	milk powder	636:646	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	1	20	theme	milk	354:357	arg1	authentication					336:349	authentication	336:349	authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA)	336:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	2	21	theme	target	607:612	arg1	class					614:618	the target class	603:618	the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	603:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	0	22	theme	forensic	220:227	arg1	evidence					229:236	forensic evidence	220:236	forensic evidence	220:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	0	23	theme	milk	186:189	arg1	profiles					174:181	pure profiles	169:181	pure profiles of milk and of both adulterants with forensic evidence	169:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	1	24	theme	independent	447:457	arg1	modeling					459:466	soft independent modeling	442:466	soft independent modeling of class analogy (DD-SIMCA)	442:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	2	25	theme	%	719:719	arg1	range					705:709	a concentration range	689:709	a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	689:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	4	26	theme	report	1137:1142	arg1	focus					1116:1120	the prime focus	1106:1120	the prime focus	1106:1120	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	5	27	dep	interferent	1567:1577	arg1	the					1535:1537	the	1535:1537	the	1535:1537	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	27	dep	interferent	1567:1577	arg1	presence					1539:1546	presence	1539:1546	presence	1539:1546	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	2	28	theme	0.8-2	714:718	arg1	w/w					722:724	w/w	722:724	w/w	722:724	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	2	28	theme	0.8-2	714:718	arg1	%					719:719	0.8-2%	714:719	0.8-2% (w/w)	714:725	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	5	29	theme	various	1644:1650	arg1	constraints					1652:1662	various constraints	1644:1662	various constraints in order	1644:1671	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	30	theme	rotational	1689:1698	arg1	ambiguity					1700:1708	the rotational ambiguity	1685:1708	the rotational ambiguity	1685:1708	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	2	31	theme	powder	641:646	arg1	samples					625:631	samples	625:631	samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	625:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	3	32	theme	further	1036:1042	arg1	investigation					1044:1056	further investigation	1036:1056	further investigation	1036:1056	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	2	33	theme	%	734:734	arg1	range					705:709	a concentration range	689:709	a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	689:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	4	34	theme	authentication	1212:1225	arg1	purposes					1227:1234	authentication purposes	1212:1234	authentication purposes	1212:1234	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	5	35	dep	studied	1431:1437	arg1	adulterants					1449:1459	adulterants	1449:1459	adulterants	1449:1459	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	35	dep	studied	1431:1437	arg1	system					1424:1429	the system	1420:1429	the system studied (milk and adulterants)	1420:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	35	dep	studied	1431:1437	arg1	milk					1440:1443	milk	1440:1443	milk	1440:1443	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	36	theme	pure	1361:1364	arg1	profiles					1366:1373	pure profiles	1361:1373	pure profiles for the contributing components contained in the system studied (milk and adulterants)	1361:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	0	37	theme	short	241:245	arg1	communication					247:259	A short communication	239:259	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.	0:260	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	2	38	theme	class	614:618	arg1	samples					558:564	all samples	554:564	all samples of genuine milk powder	554:587	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	2	38	theme	class	614:618	arg1	members					592:598	members	592:598	members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	592:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	5	39	with	analytes	1484:1491	arg1	profiles					1517:1524	strong overlapping profiles	1498:1524	strong overlapping profiles	1498:1524	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	0	40	theme	melamine	13:20	arg1	Detection					0:8	Detection	0:8	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.	0:260	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	1	41	theme	soft	442:445	arg1	modeling					459:466	soft independent modeling	442:466	soft independent modeling of class analogy (DD-SIMCA)	442:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	0	42	theme	one-class	114:122	arg1	classifier					124:133	one-class classifier	114:133	one-class classifier	114:133	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	1	43	theme	first-order	368:378	arg1	NIR					395:397	NIR	395:397	NIR	395:397	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	1	43	theme	first-order	368:378	arg1	data					414:417	first-order near-infrared (NIR) spectroscopic data	368:417	first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA)	368:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	5	44	theme	spectra	1348:1354	arg1	decomposition					1327:1339	successful bilinear data decomposition	1302:1339	successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants)	1302:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	45	theme	short	1604:1608	arg1	communication					1610:1622	this short communication	1599:1622	this short communication	1599:1622	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	1	46	theme	short	274:278	arg1	communication					280:292	The present short communication	262:292	The present short communication	262:292	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	1	47	theme	near-infrared	380:392	arg1	NIR					395:397	NIR	395:397	NIR	395:397	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	1	47	theme	near-infrared	380:392	arg1	data					414:417	first-order near-infrared (NIR) spectroscopic data	368:417	first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA)	368:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	0	48	theme	sucrose	26:32	arg1	Detection					0:8	Detection	0:8	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.	0:260	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	5	49	theme	contributing	1383:1394	arg1	components					1396:1405	the contributing components	1379:1405	the contributing components contained in the system studied (milk and adulterants)	1379:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	3	50	theme	forensic	1063:1070	arg1	point					1072:1076	forensic point	1063:1076	forensic point of view	1063:1084	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	1	51	theme	analogy	477:483	arg1	modeling					459:466	soft independent modeling	442:466	soft independent modeling of class analogy (DD-SIMCA)	442:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	1	52	theme	promising	304:312	arg1	method					325:330	a promising analytical method	302:330	a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA)	302:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	5	53	theme	uncalibrated	1554:1565	arg1	interferent					1567:1577	an uncalibrated interferent	1551:1577	an uncalibrated interferent	1551:1577	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	2	54	from	members	592:598	arg1	samples					625:631	samples	625:631	samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	625:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	5	55	theme	successful	1302:1311	arg1	decomposition					1327:1339	successful bilinear data decomposition	1302:1339	successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants)	1302:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	0	56	with	spectroscopy	84:95	arg1	DD-SIMCA					102:109	DD-SIMCA	102:109	DD-SIMCA	102:109	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	4	57	theme	chemometric	1190:1200	arg1	model					1202:1206	an appropriate chemometric model	1175:1206	an appropriate chemometric model for authentication purposes	1175:1234	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	4	58	theme	appropriate	1178:1188	arg1	model					1202:1206	an appropriate chemometric model	1175:1206	an appropriate chemometric model for authentication purposes	1175:1234	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	4	59	theme	present	1129:1135	arg1	report					1137:1142	the present report	1125:1142	the present report	1125:1142	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	3	60	theme	target	1005:1010	arg1	samples					970:976	samples	970:976	samples properly attributed in the target class or not, providing further investigation from forensic point of view	970:1084	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	60	theme	target	1005:1010	arg1	class					1012:1016	the target class	1001:1016	the target class	1001:1016	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	1	61	theme	class	471:475	arg1	DD-SIMCA					486:493	DD-SIMCA	486:493	DD-SIMCA	486:493	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	1	61	theme	class	471:475	arg1	analogy					477:483	class analogy	471:483	class analogy (DD-SIMCA)	471:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	3	62	theme	view	1081:1084	arg1	point					1072:1076	forensic point	1063:1076	forensic point of view	1063:1084	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	1	63	theme	spectroscopic	400:412	arg1	NIR					395:397	NIR	395:397	NIR	395:397	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	1	63	theme	spectroscopic	400:412	arg1	data					414:417	first-order near-infrared (NIR) spectroscopic data	368:417	first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA)	368:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	3	64	theme	samples	970:976	arg1	composition					955:965	the chemical composition	942:965	the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view	942:1084	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	4	65	theme	prime	1110:1114	arg1	focus					1116:1120	the prime focus	1106:1120	the prime focus	1106:1120	In order to extend the prime focus of the present report, which was aimed at developing an appropriate chemometric model for authentication purposes, the quantification analysis was also performed.					
32456931	3	66	theme	pure	906:909	arg1	profiles					911:918	pure profiles	906:918	pure profiles	906:918	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	5	67	theme	overlapping	1505:1515	arg1	profiles					1517:1524	strong overlapping profiles	1498:1524	strong overlapping profiles	1498:1524	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	5	68	theme	data	1322:1325	arg1	decomposition					1327:1339	successful bilinear data decomposition	1302:1339	successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants)	1302:1460	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
32456931	2	69	from	samples	625:631	arg1	class					614:618	the target class	603:618	the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	603:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	2	69	from	samples	625:631	arg1	samples					558:564	all samples	554:564	all samples of genuine milk powder	554:587	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	2	69	from	samples	625:631	arg1	members					592:598	members	592:598	members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	592:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	3	70	theme	resolution - alternating	776:799	arg1	model					868:872	a complementary chemometric model	840:872	a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles	840:918	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	70	theme	resolution - alternating	776:799	arg1	MCR-ALS					816:822	MCR-ALS	816:822	MCR-ALS	816:822	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	3	70	theme	resolution - alternating	776:799	arg1	least-squares					801:813	Multivariate curve resolution - alternating least-squares	757:813	Multivariate curve resolution - alternating least-squares (MCR-ALS)	757:823	Multivariate curve resolution - alternating least-squares (MCR-ALS) was applied as a complementary chemometric model to DD-SIMCA aimed at retrieving pure profiles, allowing to identify the chemical composition of samples properly attributed in the target class or not, providing further investigation from forensic point of view.					
32456931	0	71	theme	milk	52:55	arg1	powder					57:62	milk powder	52:62	milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence	52:236	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	1	72	theme	analytical	314:323	arg1	method					325:330	a promising analytical method	302:330	a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA)	302:494	The present short communication reports a promising analytical method for authentication of milk based on first-order near-infrared (NIR) spectroscopic data coupled to data driven soft independent modeling of class analogy (DD-SIMCA).					
32456931	0	73	dep	Detection	0:8	arg1	communication					247:259	A short communication	239:259	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.	0:260	Detection of melamine and sucrose as adulterants in milk powder using near-infrared spectroscopy with DD-SIMCA as one-class classifier and MCR-ALS as a means to provide pure profiles of milk and of both adulterants with forensic evidence: A short communication.					
32456931	2	74	theme	concentration	691:703	arg1	range					705:709	a concentration range	689:709	a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively	689:754	This one-class classifier was able to correctly classify all samples of genuine milk powder as members of the target class from samples of milk powder adulterated with melamine and sucrose in a concentration range of 0.8-2% (w/w) and 1-3% (w/w), respectively.					
32456931	5	75	theme	NIR	1344:1346	arg1	spectra					1348:1354	NIR spectra	1344:1354	NIR spectra	1344:1354	This was done by successful bilinear data decomposition of NIR spectra into pure profiles for the contributing components contained in the system studied (milk and adulterants), allowing to quantify analytes with strong overlapping profiles, even in the presence of an uncalibrated interferent, as demonstrated in this short communication using MCR-ALS under various constraints in order to decrease the rotational ambiguity.					
33418414	0	0	theme	composite	86:94	arg1	gels					96:99	rice glutelin/sugar beet pectin composite gels	54:99	rice glutelin/sugar beet pectin composite gels	54:99	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	3	1	theme	calcium	649:655	arg1	ions					657:660	calcium ions	649:660	calcium ions	649:660	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	4	2	theme	SBP	848:850	arg1	addition					836:843	the addition	832:843	the addition of SBP and calcium ions	832:867	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	1	3	from	properties	380:389	arg1	gelation					359:366	the gelation	355:366	the gelation	355:366	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	4	4	theme	scanning	963:970	arg1	microscopy					981:990	scanning electron microscopy	963:990	scanning electron microscopy	963:990	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	0	5	theme	pectin	79:84	arg1	gels					96:99	rice glutelin/sugar beet pectin composite gels	54:99	rice glutelin/sugar beet pectin composite gels	54:99	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	4	6	from	effect	822:827	arg1	formation					876:884	the formation	872:884	the formation of a denser and more homogeneous protein gel	872:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	2	7	theme	textural	548:555	arg1	properties					557:566	the rheological, textural properties	531:566	properties	557:566	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	2	8	theme	composite	609:617	arg1	gels					619:622	the RG/SBP composite gels	598:622	the RG/SBP composite gels	598:622	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	4	9	theme	ions	864:867	arg1	addition					836:843	the addition	832:843	the addition of SBP and calcium ions	832:867	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	4	10	theme	calcium	856:862	arg1	ions					864:867	calcium ions	856:867	calcium ions	856:867	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	3	11	theme	SBP	641:643	arg1	addition					629:636	The addition	625:636	The addition of SBP and calcium ions	625:660	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	2	12	theme	RG/SBP	602:607	arg1	gels					619:622	the RG/SBP composite gels	598:622	the RG/SBP composite gels	598:622	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	4	13	theme	microscopy	981:990	arg1	results					952:958	the results	948:958	the results of scanning electron microscopy	948:990	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	4	14	theme	homogeneous	907:917	arg1	gel					927:929	a denser and more homogeneous protein gel	889:929	a denser and more homogeneous protein gel	889:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	0	15	theme	gels	96:99	arg1	microstructure					36:49	microstructure	36:49	microstructure	36:49	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	0	15	theme	gels	96:99	arg1	properties					21:30	gelation properties	12:30	gelation properties	12:30	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	1	16	theme	different	304:312	arg1	0-400 mM					342:349	0-400 mM	342:349	0-400 mM	342:349	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	16	theme	different	304:312	arg1	concentrations					326:339	different calcium ion concentrations	304:339	different calcium ion concentrations (0-400 mM)	304:350	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	2	17	theme	rheological	535:545	arg1	properties					557:566	the rheological, textural properties	531:566	properties	557:566	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	4	18	theme	denser	891:896	arg1	gel					927:929	a denser and more homogeneous protein gel	889:929	a denser and more homogeneous protein gel	889:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	1	19	theme	calcium	314:320	arg1	0-400 mM					342:349	0-400 mM	342:349	0-400 mM	342:349	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	19	theme	calcium	314:320	arg1	concentrations					326:339	different calcium ion concentrations	304:339	different calcium ion concentrations (0-400 mM)	304:350	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	20	theme	rice	149:152	arg1	RG					164:165	RG	164:165	RG	164:165	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	20	theme	rice	149:152	arg1	glutelin					154:161	rice glutelin	149:161	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	21	theme	ion	322:324	arg1	0-400 mM					342:349	0-400 mM	342:349	0-400 mM	342:349	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	21	theme	ion	322:324	arg1	concentrations					326:339	different calcium ion concentrations	304:339	different calcium ion concentrations (0-400 mM)	304:350	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	2	22	theme	holding	578:584	arg1	capacity					586:593	water holding capacity	572:593	water holding capacity	572:593	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	3	23	theme	RG	761:762	arg1	properties					747:756	the gel properties	739:756	the gel properties of RG	739:762	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	4	24	theme	addition	836:843	arg1	effect					822:827	the positive effect	809:827	the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel	809:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	1	25	theme	glutelin	154:161	arg1	gels					202:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	26	theme	concentrations	326:339	arg1	effects					293:299	the effects	289:299	the effects of different calcium ion concentrations (0-400 mM) on the gelation	289:366	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	26	theme	concentrations	326:339	arg1	microstructure					395:408	microstructure	395:408	microstructure of the RG/SBP composite gels	395:437	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	26	theme	concentrations	326:339	arg1	properties					380:389	structural properties	369:389	structural properties	369:389	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	2	27	theme	water	572:576	arg1	capacity					586:593	water holding capacity	572:593	water holding capacity	572:593	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	1	28	theme	RG/SBP	417:422	arg1	gels					434:437	the RG/SBP composite gels	413:437	the RG/SBP composite gels	413:437	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	4	29	from	changes	769:775	arg1	spectroscopy					786:797	Raman spectroscopy	780:797	Raman spectroscopy	780:797	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	3	30	theme	hydrophobic	675:685	arg1	interaction					687:697	the hydrophobic interaction	671:697	the hydrophobic interaction between RG molecules	671:718	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	0	31	theme	Structural	0:9	arg1	properties					21:30	gelation properties	12:30	gelation properties	12:30	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	1	32	theme	laccase	224:230	arg1	cross-linking					240:252	laccase induced cross-linking	224:252	laccase induced cross-linking	224:252	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	3	33	theme	RG	707:708	arg1	molecules					710:718	RG molecules	707:718	RG molecules	707:718	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	1	34	theme	composite	424:432	arg1	gels					434:437	the RG/SBP composite gels	413:437	the RG/SBP composite gels	413:437	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	4	35	theme	positive	813:820	arg1	effect					822:827	the positive effect	809:827	the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel	809:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	1	36	theme	induced	232:238	arg1	cross-linking					240:252	laccase induced cross-linking	224:252	laccase induced cross-linking	224:252	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	0	37	theme	strengths	119:127	arg1	Effects					102:108	Effects	102:108	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.	0:128	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	1	38	theme	gels	434:437	arg1	effects					293:299	the effects	289:299	the effects of different calcium ion concentrations (0-400 mM) on the gelation	289:366	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	38	theme	gels	434:437	arg1	microstructure					395:408	microstructure	395:408	microstructure of the RG/SBP composite gels	395:437	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	38	theme	gels	434:437	arg1	properties					380:389	structural properties	369:389	structural properties	369:389	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	0	39	theme	gelation	12:19	arg1	properties					21:30	gelation properties	12:30	gelation properties	12:30	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	0	40	theme	ionic	113:117	arg1	strengths					119:127	ionic strengths	113:127	ionic strengths	113:127	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	1	41	theme	/sugar	167:172	arg1	gels					202:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	42	from	effects	293:299	arg1	gelation					359:366	the gelation	355:366	the gelation	355:366	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	43	theme	beet	174:177	arg1	gels					202:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	4	44	theme	gel	927:929	arg1	formation					876:884	the formation	872:884	the formation of a denser and more homogeneous protein gel	872:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	1	45	theme	subsequent	258:267	arg1	treatment					274:282	subsequent heat treatment	258:282	subsequent heat treatment	258:282	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	46	theme	pectin	179:184	arg1	gels					202:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	0	47	theme	rice	54:57	arg1	gels					96:99	rice glutelin/sugar beet pectin composite gels	54:99	rice glutelin/sugar beet pectin composite gels	54:99	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	1	48	theme	heat	269:272	arg1	treatment					274:282	subsequent heat treatment	258:282	subsequent heat treatment	258:282	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	5	49	theme	calcium	1010:1016	arg1	ions					1018:1021	calcium ions	1010:1021	calcium ions	1010:1021	Overall, SBP and calcium ions could be applied to the plant protein gel systems as gel-strengthening agents.					
33418414	3	50	theme	ions	657:660	arg1	addition					629:636	The addition	625:636	The addition of SBP and calcium ions	625:660	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	5	51	theme	plant	1047:1051	arg1	systems					1065:1071	the plant protein gel systems	1043:1071	the plant protein gel systems as gel-strengthening agents	1043:1099	Overall, SBP and calcium ions could be applied to the plant protein gel systems as gel-strengthening agents.					
33418414	2	52	theme	calcium	505:511	arg1	ion					513:515	200 mM calcium ion	498:515	200 mM calcium ion	498:515	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	4	53	theme	Raman	780:784	arg1	spectroscopy					786:797	Raman spectroscopy	780:797	Raman spectroscopy	780:797	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	5	54	theme	protein	1053:1059	arg1	systems					1065:1071	the plant protein gel systems	1043:1071	the plant protein gel systems as gel-strengthening agents	1043:1099	Overall, SBP and calcium ions could be applied to the plant protein gel systems as gel-strengthening agents.					
33418414	2	55	theme	gels	619:622	arg1	properties					557:566	the rheological, textural properties	531:566	properties	557:566	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	2	55	theme	gels	619:622	arg1	capacity					586:593	water holding capacity	572:593	water holding capacity	572:593	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	0	56	dep	properties	21:30	arg1	Effects					102:108	Effects	102:108	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.	0:128	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	5	57	theme	gel	1061:1063	arg1	systems					1065:1071	the plant protein gel systems	1043:1071	the plant protein gel systems as gel-strengthening agents	1043:1099	Overall, SBP and calcium ions could be applied to the plant protein gel systems as gel-strengthening agents.					
33418414	2	58	theme	ion	513:515	arg1	addition					486:493	the addition	482:493	the addition of 200 mM calcium ion	482:515	The results showed that the addition of 200 mM calcium ion could improve the rheological, textural properties and water holding capacity of the RG/SBP composite gels.					
33418414	1	59	theme	SBP	187:189	arg1	gels					202:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	0	60	theme	beet	74:77	arg1	gels					96:99	rice glutelin/sugar beet pectin composite gels	54:99	rice glutelin/sugar beet pectin composite gels	54:99	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	1	61	theme	structural	369:378	arg1	properties					380:389	structural properties	369:389	structural properties	369:389	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	1	62	from	microstructure	395:408	arg1	gelation					359:366	the gelation	355:366	the gelation	355:366	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
33418414	5	63	theme	gel-strengthening	1076:1092	arg1	agents					1094:1099	gel-strengthening agents	1076:1099	gel-strengthening agents	1076:1099	Overall, SBP and calcium ions could be applied to the plant protein gel systems as gel-strengthening agents.					
33418414	3	64	theme	gel	743:745	arg1	properties					747:756	the gel properties	739:756	the gel properties of RG	739:762	The addition of SBP and calcium ions enhanced the hydrophobic interaction between RG molecules, thereby increased the gel properties of RG.					
33418414	0	65	theme	glutelin/sugar	59:72	arg1	gels					96:99	rice glutelin/sugar beet pectin composite gels	54:99	rice glutelin/sugar beet pectin composite gels	54:99	Structural, gelation properties and microstructure of rice glutelin/sugar beet pectin composite gels: Effects of ionic strengths.					
33418414	4	66	theme	protein	919:925	arg1	gel					927:929	a denser and more homogeneous protein gel	889:929	a denser and more homogeneous protein gel	889:929	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	4	67	theme	electron	972:979	arg1	microscopy					981:990	scanning electron microscopy	963:990	scanning electron microscopy	963:990	The changes in Raman spectroscopy reflected the positive effect of the addition of SBP and calcium ions on the formation of a denser and more homogeneous protein gel, as evidenced by the results of scanning electron microscopy.					
33418414	1	68	theme	composite	192:200	arg1	gels					202:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	the rice glutelin (RG)/sugar beet pectin (SBP) composite gels	145:205	In this study, the rice glutelin (RG)/sugar beet pectin (SBP) composite gels were prepared by laccase induced cross-linking and subsequent heat treatment, and the effects of different calcium ion concentrations (0-400 mM) on the gelation, structural properties and microstructure of the RG/SBP composite gels were investigated.					
32758610	0	0	theme	biological	100:109	arg1	properties					111:120	improved biological properties	91:120	improved biological properties	91:120	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	0	1	with	hydrogel	45:52	arg1	properties					76:85	tunable physical properties	59:85	tunable physical properties	59:85	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	0	1	with	hydrogel	45:52	arg1	properties					111:120	improved biological properties	91:120	improved biological properties	91:120	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	6	2	theme	self-crosslinkable	1046:1063	arg1	hydrogels					1080:1088	self-crosslinkable and injectable hydrogels	1046:1088	self-crosslinkable and injectable hydrogels	1046:1088	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	0	3	theme	improved	91:98	arg1	properties					111:120	improved biological properties	91:120	improved biological properties	91:120	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	4	4	dep	more	795:798	arg1	matrix					819:824	cartilage specific matrix	800:824	cartilage specific matrix	800:824	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	4	5	theme	cartilage	800:808	arg1	matrix					819:824	cartilage specific matrix	800:824	cartilage specific matrix	800:824	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	1	6	theme	physiological	282:294	arg1	conditions					296:305	physiological conditions	282:305	physiological conditions without any initiators or crosslinkers	282:344	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	2	7	theme	HA-sNHS	536:542	arg1	DS					529:530	DS	529:530	DS	529:530	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	7	theme	HA-sNHS	536:542	arg1	degree					521:526	the substitution degree	504:526	the substitution degree (DS) of HA-sNHS	504:542	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	7	8	theme	engineering	1232:1242	arg1	fields					1192:1197	the fields	1188:1197	the fields of cell encapsulation and tissue engineering	1188:1242	It implied that these hydrogels could find some applications in the fields of cell encapsulation and tissue engineering.					
32758610	4	9	theme	Col	734:736	arg1	%					749:749	hydrogel Col I/HA-sNHS32%	725:749	hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32%	725:784	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	7	10	theme	cell	1202:1205	arg1	encapsulation					1207:1219	cell encapsulation	1202:1219	cell encapsulation	1202:1219	It implied that these hydrogels could find some applications in the fields of cell encapsulation and tissue engineering.					
32758610	7	11	theme	encapsulation	1207:1219	arg1	fields					1192:1197	the fields	1188:1197	the fields of cell encapsulation and tissue engineering	1188:1242	It implied that these hydrogels could find some applications in the fields of cell encapsulation and tissue engineering.					
32758610	4	12	theme	specific	810:817	arg1	matrix					819:824	cartilage specific matrix	800:824	cartilage specific matrix	800:824	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	1	13	theme	Col	205:207	arg1	I					209:209	Col I	205:209	Col I	205:209	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	1	13	theme	Col	205:207	arg1	type					197:200	collagen type I	188:202	collagen type I (Col I)	188:210	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	0	14	theme	hyaluronic	10:19	arg1	hydrogel					45:52	hyaluronic acid/collagen composite hydrogel	10:52	hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties	10:120	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	4	15	from	%	784:784	arg1	%					749:749	hydrogel Col I/HA-sNHS32%	725:749	hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32%	725:784	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	2	16	theme	physical	351:358	arg1	properties					360:369	The physical properties	347:369	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property	347:470	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	4	17	from	chondrocytes	709:720	arg1	%					749:749	hydrogel Col I/HA-sNHS32%	725:749	hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32%	725:784	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	2	18	theme	swelling	408:415	arg1	property					417:424	swelling property	408:424	swelling property	408:424	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	18	theme	swelling	408:415	arg1	hydrogels					374:382	hydrogels	374:382	hydrogels	374:382	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	18	theme	swelling	408:415	arg1	time					402:405	gelation time	393:405	gelation time	393:405	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	4	19	theme	hydrogel	725:732	arg1	%					749:749	hydrogel Col I/HA-sNHS32%	725:749	hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32%	725:784	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	0	20	theme	composite	35:43	arg1	hydrogel					45:52	hyaluronic acid/collagen composite hydrogel	10:52	hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties	10:120	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	6	21	theme	simple	1013:1018	arg1	method					1020:1025	a novel and simple method	1001:1025	a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties	1001:1121	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	1	22	theme	Self-crosslinkable	123:140	arg1	hydrogels					157:165	Self-crosslinkable and injectable hydrogels	123:165	Self-crosslinkable and injectable hydrogels	123:165	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	2	23	theme	mechanical	452:461	arg1	hydrogels					374:382	hydrogels	374:382	hydrogels	374:382	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	23	theme	mechanical	452:461	arg1	property					463:470	mechanical property	452:470	mechanical property	452:470	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	23	theme	mechanical	452:461	arg1	time					402:405	gelation time	393:405	gelation time	393:405	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	0	24	theme	acid/collagen	21:33	arg1	hydrogel					45:52	hyaluronic acid/collagen composite hydrogel	10:52	hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties	10:120	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	5	25	theme	good	936:939	arg1	biodegradability					941:956	good biodegradability	936:956	good biodegradability	936:956	The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility.					
32758610	1	26	theme	N-hydroxy	216:224	arg1	sulfosuccinimide					226:241	N-hydroxy sulfosuccinimide	216:241	N-hydroxy sulfosuccinimide	216:241	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	2	27	theme	substitution	508:519	arg1	DS					529:530	DS	529:530	DS	529:530	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	27	theme	substitution	508:519	arg1	degree					521:526	the substitution degree	504:526	the substitution degree (DS) of HA-sNHS	504:542	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	6	28	theme	novel	1003:1007	arg1	method					1020:1025	a novel and simple method	1001:1025	a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties	1001:1121	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	6	29	theme	physical	1103:1110	arg1	properties					1112:1121	tunable physical properties	1095:1121	tunable physical properties	1095:1121	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	7	30	from	applications	1172:1183	arg1	fields					1192:1197	the fields	1188:1197	the fields of cell encapsulation and tissue engineering	1188:1242	It implied that these hydrogels could find some applications in the fields of cell encapsulation and tissue engineering.					
32758610	5	31	theme	Col	910:912	arg1	%					925:925	Col I and Col I/HA-sNHS32%	900:925	Col I and Col I/HA-sNHS32%	900:925	The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility.					
32758610	3	32	theme	matrix	642:647	arg1	secretion					649:657	matrix secretion	642:657	matrix secretion	642:657	Chondrocytes were encapsulated into hydrogels and their proliferation, phenotype maintenance and matrix secretion were characterized.					
32758610	2	33	theme	degradation	427:437	arg1	hydrogels					374:382	hydrogels	374:382	hydrogels	374:382	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	33	theme	degradation	427:437	arg1	property					439:446	degradation property	427:446	degradation property	427:446	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	2	33	theme	degradation	427:437	arg1	time					402:405	gelation time	393:405	gelation time	393:405	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	0	34	theme	physical	67:74	arg1	properties					76:85	tunable physical properties	59:85	tunable physical properties	59:85	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	6	35	theme	tunable	1095:1101	arg1	properties					1112:1121	tunable physical properties	1095:1121	tunable physical properties	1095:1121	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	3	36	theme	phenotype	616:624	arg1	maintenance					626:636	phenotype maintenance	616:636	phenotype maintenance	616:636	Chondrocytes were encapsulated into hydrogels and their proliferation, phenotype maintenance and matrix secretion were characterized.					
32758610	1	37	theme	injectable	146:155	arg1	hydrogels					157:165	Self-crosslinkable and injectable hydrogels	123:165	Self-crosslinkable and injectable hydrogels	123:165	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	0	38	theme	tunable	59:65	arg1	properties					76:85	tunable physical properties	59:85	tunable physical properties	59:85	Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.					
32758610	5	39	theme	animal	854:859	arg1	experiment					861:870	animal experiment	854:870	animal experiment	854:870	The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility.					
32758610	2	40	theme	hydrogels	374:382	arg1	properties					360:369	The physical properties	347:369	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property	347:470	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
32758610	7	41	theme	tissue	1225:1230	arg1	engineering					1232:1242	tissue engineering	1225:1242	tissue engineering	1225:1242	It implied that these hydrogels could find some applications in the fields of cell encapsulation and tissue engineering.					
32758610	6	42	with	fabrication	1031:1041	arg1	properties					1112:1121	tunable physical properties	1095:1121	tunable physical properties	1095:1121	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	5	43	dep	hydrogels	890:898	arg1	both					927:930	both	927:930	both	927:930	The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility.					
32758610	4	44	theme	HA-sNHS	770:776	arg1	%					784:784	32%	782:784	32%	782:784	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	4	44	theme	HA-sNHS	770:776	arg1	DS					764:765	the DS	760:765	the DS of HA-sNHS	760:776	The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others.					
32758610	1	45	theme	hyaluronic	253:262	arg1	HA-sNHS					270:276	HA-sNHS	270:276	HA-sNHS	270:276	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	1	45	theme	hyaluronic	253:262	arg1	acid					264:267	hyaluronic acid	253:267	hyaluronic acid (HA-sNHS)	253:277	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	1	46	theme	collagen	188:195	arg1	I					209:209	Col I	205:209	Col I	205:209	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	1	46	theme	collagen	188:195	arg1	type					197:200	collagen type I	188:202	collagen type I (Col I)	188:210	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	6	47	theme	hydrogels	1080:1088	arg1	fabrication					1031:1041	fabrication	1031:1041	fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties	1031:1121	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	5	48	theme	Col	900:902	arg1	%					925:925	Col I and Col I/HA-sNHS32%	900:925	Col I and Col I/HA-sNHS32%	900:925	The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility.					
32758610	6	49	theme	injectable	1069:1078	arg1	hydrogels					1080:1088	self-crosslinkable and injectable hydrogels	1046:1088	self-crosslinkable and injectable hydrogels	1046:1088	This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties.					
32758610	1	50	dep	initiators	319:328	arg1	any					315:317	any	315:317	any	315:317	Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers.					
32758610	5	51	theme	experiment	861:870	arg1	results					843:849	The results	839:849	The results of animal experiment	839:870	The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility.					
32758610	2	52	theme	gelation	393:400	arg1	time					402:405	gelation time	393:405	gelation time	393:405	The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS.					
31972193	2	0	theme	different	361:369	arg1	concentrations					371:384	different concentrations	361:384	different concentrations of zein nanoparticles	361:406	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	2	1	from	effect	317:322	arg1	properties					449:458	the structural and physico-mechanical properties	411:458	the structural and physico-mechanical properties of plasticized potato starch films	411:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	4	2	theme	Barrier	554:560	arg1	properties					562:571	Barrier properties	554:571	Barrier properties	554:571	Barrier properties were further improved with increasing ZNP content.					
31972193	9	3	theme	olive	1197:1201	arg1	oil					1203:1205	olive oil	1197:1205	olive oil	1197:1205	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	7	4	theme	ZNP	908:910	arg1	use					901:903	the use	897:903	the use of ZNP and olive oil	897:924	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	8	5	dep	microstructure	931:944	arg1	The					927:929	The	927:929	The	927:929	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	0	6	theme	zein	101:104	arg1	nanoparticles					106:118	zein nanoparticles	101:118	zein nanoparticles	101:118	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	5	7	theme	tensile	651:657	arg1	strength					659:666	the tensile strength	647:666	the tensile strength	647:666	It was discovered that the tensile strength increased as the ZNP content increased, but decreased with incorporation of the oil.					
31972193	3	8	theme	oil	540:542	arg1	addition					544:551	oil addition	540:551	oil addition	540:551	The water vapor permeability decreased with oil addition.					
31972193	6	9	theme	Contact	753:759	arg1	angle					761:765	Contact angle	753:765	Contact angle	753:765	Contact angle increased with ZNP and olive oil addition.					
31972193	1	10	theme	bio-composite	136:148	arg1	films					150:154	bio-composite films	136:154	bio-composite films	136:154	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	8	11	theme	force	1075:1079	arg1	AFM					1093:1095	AFM	1093:1095	AFM	1093:1095	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	8	11	theme	force	1075:1079	arg1	microscopy					1081:1090	atomic force microscopy	1068:1090	atomic force microscopy (AFM)	1068:1096	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	2	12	theme	physico-mechanical	430:447	arg1	properties					449:458	the structural and physico-mechanical properties	411:458	the structural and physico-mechanical properties of plasticized potato starch films	411:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	2	13	theme	films	489:493	arg1	properties					449:458	the structural and physico-mechanical properties	411:458	the structural and physico-mechanical properties of plasticized potato starch films	411:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	8	14	theme	atomic	1068:1073	arg1	AFM					1093:1095	AFM	1093:1095	AFM	1093:1095	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	8	14	theme	atomic	1068:1073	arg1	microscopy					1081:1090	atomic force microscopy	1068:1090	atomic force microscopy (AFM)	1068:1096	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	8	15	theme	scanning	1029:1036	arg1	SEM					1059:1061	SEM	1059:1061	SEM	1059:1061	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	8	15	theme	scanning	1029:1036	arg1	microscopy					1047:1056	scanning electron microscopy	1029:1056	scanning electron microscopy (SEM)	1029:1062	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	0	16	theme	Structural	0:9	arg1	properties					34:43	Structural and physico-mechanical properties	0:43	Structural and physico-mechanical properties of potato starch-olive oil edible films	0:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	1	17	theme	film-forming	182:193	arg1	emulsions					195:203	film-forming emulsions	182:203	film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP)	182:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	2	18	theme	starch	482:487	arg1	films					489:493	plasticized potato starch films	463:493	plasticized potato starch films	463:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	9	19	theme	storage	1149:1155	arg1	modulus					1157:1163	the storage modulus	1145:1163	the storage modulus decreased with incorporation of olive oil, however	1145:1214	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	2	20	theme	combined	331:338	arg1	use					340:342	the combined use	327:342	the combined use of olive oil and different concentrations of zein nanoparticles	327:406	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	9	21	theme	Dynamic	1099:1105	arg1	test					1126:1129	Dynamic mechanical thermal test	1099:1129	Dynamic mechanical thermal test	1099:1129	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	2	22	theme	nanoparticles	394:406	arg1	oil					353:355	olive oil	347:355	olive oil	347:355	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	2	22	theme	nanoparticles	394:406	arg1	concentrations					371:384	different concentrations	361:384	different concentrations of zein nanoparticles	361:406	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	2	23	theme	potato	475:480	arg1	films					489:493	plasticized potato starch films	463:493	plasticized potato starch films	463:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	5	24	theme	ZNP	685:687	arg1	content					689:695	the ZNP content	681:695	the ZNP content	681:695	It was discovered that the tensile strength increased as the ZNP content increased, but decreased with incorporation of the oil.					
31972193	9	25	theme	mechanical	1107:1116	arg1	test					1126:1129	Dynamic mechanical thermal test	1099:1129	Dynamic mechanical thermal test	1099:1129	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	2	26	theme	zein	389:392	arg1	nanoparticles					394:406	zein nanoparticles	389:406	zein nanoparticles	389:406	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	2	27	theme	plasticized	463:473	arg1	films					489:493	plasticized potato starch films	463:493	plasticized potato starch films	463:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	7	28	theme	potato	849:854	arg1	film					872:875	potato starch-glycerol film	849:875	potato starch-glycerol film	849:875	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	8	29	theme	electron	1038:1045	arg1	SEM					1059:1061	SEM	1059:1061	SEM	1059:1061	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	8	29	theme	electron	1038:1045	arg1	microscopy					1047:1056	scanning electron microscopy	1029:1056	scanning electron microscopy (SEM)	1029:1062	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	9	30	theme	oil	1203:1205	arg1	incorporation					1180:1192	incorporation	1180:1192	incorporation of olive oil	1180:1205	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	9	31	theme	thermal	1118:1124	arg1	test					1126:1129	Dynamic mechanical thermal test	1099:1129	Dynamic mechanical thermal test	1099:1129	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	0	32	theme	physico-mechanical	15:32	arg1	properties					34:43	Structural and physico-mechanical properties	0:43	Structural and physico-mechanical properties of potato starch-olive oil edible films	0:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	7	33	theme	UV	829:830	arg1	transmittance					832:844	UV transmittance	829:844	UV transmittance	829:844	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	5	34	theme	oil	748:750	arg1	incorporation					727:739	incorporation	727:739	incorporation of the oil	727:750	It was discovered that the tensile strength increased as the ZNP content increased, but decreased with incorporation of the oil.					
31972193	9	35	theme	nanoparticles	1243:1255	arg1	content					1257:1263	nanoparticles content	1243:1263	nanoparticles content	1243:1263	Dynamic mechanical thermal test revealed that the storage modulus decreased with incorporation of olive oil, however was improved by increasing nanoparticles content.					
31972193	8	36	dep	infrared	1012:1019	arg1	FTIR					1022:1025	FTIR	1022:1025	FTIR	1022:1025	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	7	37	theme	starch-glycerol	856:870	arg1	film					872:875	potato starch-glycerol film	849:875	potato starch-glycerol film	849:875	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	1	38	theme	potato	214:219	arg1	oil					243:245	potato starch-glycerol-olive oil	214:245	potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP)	214:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	0	39	theme	potato	48:53	arg1	films					79:83	potato starch-olive oil edible films	48:83	potato starch-olive oil edible films	48:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	4	40	theme	ZNP	611:613	arg1	content					615:621	ZNP content	611:621	ZNP content	611:621	Barrier properties were further improved with increasing ZNP content.					
31972193	7	41	theme	film	872:875	arg1	color					819:823	Apparent color	810:823	Apparent color	810:823	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	7	41	theme	film	872:875	arg1	transmittance					832:844	UV transmittance	829:844	UV transmittance	829:844	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	1	42	theme	starch-glycerol-olive	221:241	arg1	oil					243:245	potato starch-glycerol-olive oil	214:245	potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP)	214:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	7	43	theme	oil	922:924	arg1	use					901:903	the use	897:903	the use of ZNP and olive oil	897:924	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	2	44	theme	structural	415:424	arg1	properties					449:458	the structural and physico-mechanical properties	411:458	the structural and physico-mechanical properties of plasticized potato starch films	411:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	0	45	theme	oil	68:70	arg1	films					79:83	potato starch-olive oil edible films	48:83	potato starch-olive oil edible films	48:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	6	46	theme	oil	796:798	arg1	addition					800:807	olive oil addition	790:807	olive oil addition	790:807	Contact angle increased with ZNP and olive oil addition.					
31972193	2	47	theme	oil	353:355	arg1	use					340:342	the combined use	327:342	the combined use of olive oil and different concentrations of zein nanoparticles	327:406	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	0	48	theme	starch-olive	55:66	arg1	films					79:83	potato starch-olive oil edible films	48:83	potato starch-olive oil edible films	48:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	1	49	contain	containing	247:256	arg2	ZNP					288:290	ZNP	288:290	ZNP	288:290	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	1	49	contain	containing	247:256	arg2	nanoparticles					273:285	dispersed zein nanoparticles	258:285	dispersed zein nanoparticles (ZNP)	258:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	1	49	contain	containing	247:256	arg1	oil					243:245	potato starch-glycerol-olive oil	214:245	potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP)	214:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	6	50	theme	olive	790:794	arg1	addition					800:807	olive oil addition	790:807	olive oil addition	790:807	Contact angle increased with ZNP and olive oil addition.					
31972193	2	51	theme	olive	347:351	arg1	oil					353:355	olive oil	347:355	olive oil	347:355	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	8	52	dep	transform	1002:1010	arg1	infrared					1012:1019	infrared	1012:1019	infrared	1012:1019	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	7	53	theme	Apparent	810:817	arg1	color					819:823	Apparent color	810:823	Apparent color	810:823	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
31972193	8	54	theme	films	966:970	arg1	morphologies					950:961	morphologies	950:961	morphologies	950:961	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	8	54	theme	films	966:970	arg1	microstructure					931:944	microstructure	931:944	microstructure	931:944	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	3	55	theme	vapor	506:510	arg1	permeability					512:523	The water vapor permeability	496:523	The water vapor permeability	496:523	The water vapor permeability decreased with oil addition.					
31972193	8	56	dep	Fourier	994:1000	arg1	transform					1002:1010	transform	1002:1010	transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM)	1002:1096	The microstructure and morphologies of films were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).					
31972193	2	57	theme	use	340:342	arg1	effect					317:322	the effect	313:322	the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films	313:493	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	3	58	theme	water	500:504	arg1	permeability					512:523	The water vapor permeability	496:523	The water vapor permeability	496:523	The water vapor permeability decreased with oil addition.					
31972193	1	59	theme	dispersed	258:266	arg1	ZNP					288:290	ZNP	288:290	ZNP	288:290	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	1	59	theme	dispersed	258:266	arg1	nanoparticles					273:285	dispersed zein nanoparticles	258:285	dispersed zein nanoparticles (ZNP)	258:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	0	60	theme	films	79:83	arg1	properties					34:43	Structural and physico-mechanical properties	0:43	Structural and physico-mechanical properties of potato starch-olive oil edible films	0:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	1	61	theme	zein	268:271	arg1	ZNP					288:290	ZNP	288:290	ZNP	288:290	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	1	61	theme	zein	268:271	arg1	nanoparticles					273:285	dispersed zein nanoparticles	258:285	dispersed zein nanoparticles (ZNP)	258:291	In this study, bio-composite films were developed by casting film-forming emulsions based on potato starch-glycerol-olive oil containing dispersed zein nanoparticles (ZNP).					
31972193	0	62	theme	edible	72:77	arg1	films					79:83	potato starch-olive oil edible films	48:83	potato starch-olive oil edible films	48:83	Structural and physico-mechanical properties of potato starch-olive oil edible films reinforced with zein nanoparticles.					
31972193	2	63	theme	concentrations	371:384	arg1	use					340:342	the combined use	327:342	the combined use of olive oil and different concentrations of zein nanoparticles	327:406	This paper studied the effect of the combined use of olive oil and different concentrations of zein nanoparticles on the structural and physico-mechanical properties of plasticized potato starch films.					
31972193	7	64	theme	olive	916:920	arg1	oil					922:924	olive oil	916:924	olive oil	916:924	Apparent color and UV transmittance of potato starch-glycerol film were impressed with the use of ZNP and olive oil.					
33731198	7	0	theme	0.05:0.15:0.76:0.04	1279:1297	arg1	ratio					1270:1274	a molar ratio	1262:1274	a molar ratio of 0.05:0.15:0.76:0.04	1262:1297	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	6	1	theme	molecular	973:981	arg1	3.235 × 103 Da					1052:1065	3.235 × 103 Da	1052:1065	3.235 × 103 Da	1052:1065	The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively.					
33731198	6	1	theme	molecular	973:981	arg1	weight					983:988	The molecular weight	969:988	The molecular weight of the two water-soluble polysaccharides	969:1029	The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively.					
33731198	8	2	from	mannose	1347:1353	arg1	ratio					1397:1401	a molar ratio	1389:1401	a molar ratio of 0.04:0.17:0.79	1389:1419	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	1	3	theme	important	174:182	arg1	class					184:188	a very important class	167:188	a very important class of biological macromolecules in nature	167:227	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	3	4	from	betulina	584:591	arg1	polysaccharides					554:568	polysaccharides	554:568	polysaccharides from Lenzites betulina (LBPs)	554:598	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	4	from	betulina	584:591	arg1	characteristics					510:524	the structural characteristics	495:524	the structural characteristics	495:524	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	4	from	betulina	584:591	arg1	activity					542:549	antioxidant activity	530:549	antioxidant activity	530:549	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	0	5	theme	polysaccharides	83:97	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	0	5	theme	polysaccharides	83:97	arg1	characteristics					21:35	structure characteristics	11:35	structure characteristics	11:35	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	0	5	theme	polysaccharides	83:97	arg1	activity					53:60	antioxidant activity	41:60	antioxidant activity	41:60	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	5	6	dep	Fourier	822:828	arg1	transform					830:838	transform	830:838	transform infrared spectrometry (FT-IR)	830:868	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	3	7	from	activity	542:549	arg1	LBPs					594:597	LBPs	594:597	LBPs	594:597	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	7	from	activity	542:549	arg1	betulina					584:591	Lenzites betulina	575:591	Lenzites betulina (LBPs)	575:598	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	5	8	theme	polysaccharides	952:966	arg1	peaks					943:947	characteristic peaks	928:947	characteristic peaks of polysaccharides	928:966	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	0	9	from	Isolation	0:8	arg1	betulina					113:120	betulina	113:120	betulina	113:120	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	7	10	theme	LBPs-5	1198:1203	arg1	compositions					1182:1193	the monosaccharide compositions	1163:1193	the monosaccharide compositions of LBPs-5	1163:1203	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	7	10	theme	LBPs-5	1198:1203	arg1	mannose					1210:1216	mannose	1210:1216	mannose	1210:1216	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	1	11	from	class	184:188	arg1	nature					222:227	nature	222:227	nature	222:227	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	2	12	theme	biological	320:329	arg1	activity					331:338	biological activity	320:338	biological activity	320:338	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	4	13	theme	sevag	635:639	arg1	method					641:646	sevag method	635:646	sevag method	635:646	The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6.					
33731198	8	14	from	acid	1367:1370	arg1	ratio					1397:1401	a molar ratio	1389:1401	a molar ratio of 0.04:0.17:0.79	1389:1419	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	10	15	contain	had	1707:1709	arg1	LBPs					1702:1705	LBPs	1702:1705	LBPs	1702:1705	CONCLUSIONS The outcome of the study indicated that LBPs had good potential as medicine and food.					
33731198	10	15	contain	had	1707:1709	arg2	potential					1716:1724	good potential	1711:1724	good potential	1711:1724	CONCLUSIONS The outcome of the study indicated that LBPs had good potential as medicine and food.					
33731198	2	16	theme	research	389:396	arg1	hotspots					398:405	the research hotspots	385:405	the research hotspots of scholars	385:417	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	0	17	from	activity	53:60	arg1	betulina					113:120	betulina	113:120	betulina	113:120	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	9	18	theme	total	1543:1547	arg1	power					1558:1562	weak total reducing power	1538:1562	weak total reducing power	1538:1562	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	3	19	theme	Lenzites	575:582	arg1	LBPs					594:597	LBPs	594:597	LBPs	594:597	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	19	theme	Lenzites	575:582	arg1	betulina					584:591	Lenzites betulina	575:591	Lenzites betulina (LBPs)	575:598	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	7	20	theme	molar	1264:1268	arg1	ratio					1270:1274	a molar ratio	1262:1274	a molar ratio of 0.05:0.15:0.76:0.04	1262:1297	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	7	21	from	galactose	1249:1257	arg1	ratio					1270:1274	a molar ratio	1262:1274	a molar ratio of 0.05:0.15:0.76:0.04	1262:1297	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	7	22	theme	monosaccharide	1167:1180	arg1	compositions					1182:1193	the monosaccharide compositions	1163:1193	the monosaccharide compositions of LBPs-5	1163:1203	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	7	22	theme	monosaccharide	1167:1180	arg1	mannose					1210:1216	mannose	1210:1216	mannose	1210:1216	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	1	23	theme	biological	193:202	arg1	macromolecules					204:217	biological macromolecules	193:217	biological macromolecules in nature	193:227	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	10	24	theme	study	1681:1685	arg1	outcome					1666:1672	The outcome	1662:1672	CONCLUSIONS The outcome of the study	1650:1685	CONCLUSIONS The outcome of the study indicated that LBPs had good potential as medicine and food.					
33731198	3	25	dep	RESULTS	439:445	arg1	performed					462:470	performed	462:470	was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs)	458:598	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	26	from	characteristics	510:524	arg1	LBPs					594:597	LBPs	594:597	LBPs	594:597	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	26	from	characteristics	510:524	arg1	betulina					584:591	Lenzites betulina	575:591	Lenzites betulina (LBPs)	575:598	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	2	27	theme	polysaccharides	350:364	arg1	structure					306:314	structure	306:314	structure	306:314	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	2	27	theme	polysaccharides	350:364	arg1	activity					331:338	biological activity	320:338	biological activity	320:338	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	1	28	theme	macromolecules	204:217	arg1	class					184:188	a very important class	167:188	a very important class of biological macromolecules in nature	167:227	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	9	29	from	effects	1504:1510	arg1	·OH					1530:1532	·OH	1530:1532	·OH	1530:1532	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	9	29	from	effects	1504:1510	arg1	ABTS·+					1522:1527	ABTS·+	1522:1527	ABTS·+	1522:1527	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	9	29	from	effects	1504:1510	arg1	DPPH·					1515:1519	DPPH·	1515:1519	DPPH·	1515:1519	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	9	29	from	effects	1504:1510	arg1	power					1558:1562	weak total reducing power	1538:1562	weak total reducing power	1538:1562	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	5	30	dep	transform	830:838	arg1	infrared					840:847	infrared	840:847	transform infrared spectrometry (FT-IR)	830:868	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	2	31	theme	fungal	343:348	arg1	polysaccharides					350:364	fungal polysaccharides	343:364	fungal polysaccharides	343:364	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	2	32	theme	scholars	410:417	arg1	hotspots					398:405	the research hotspots	385:405	the research hotspots of scholars	385:417	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	7	33	with	HPLC	1110:1113	arg1	analysis					1139:1146	PMP derivatization analysis	1120:1146	PMP derivatization analysis	1120:1146	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	8	34	theme	molar	1391:1395	arg1	ratio					1397:1401	a molar ratio	1389:1401	a molar ratio of 0.04:0.17:0.79	1389:1419	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	9	35	theme	weak	1538:1541	arg1	power					1558:1562	weak total reducing power	1538:1562	weak total reducing power	1538:1562	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	0	36	theme	structure	11:19	arg1	characteristics					21:35	structure characteristics	11:35	structure characteristics	11:35	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	1	37	theme	BACKGROUND	123:132	arg1	polysaccharides					141:155	BACKGROUND Fungal polysaccharides	123:155	BACKGROUND Fungal polysaccharides	123:155	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	2	38	from	studies	295:301	arg1	structure					306:314	structure	306:314	structure	306:314	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	2	38	from	studies	295:301	arg1	activity					331:338	biological activity	320:338	biological activity	320:338	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	5	39	theme	characteristic	928:941	arg1	peaks					943:947	characteristic peaks	928:947	characteristic peaks of polysaccharides	928:966	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	0	40	theme	antioxidant	41:51	arg1	activity					53:60	antioxidant activity	41:60	antioxidant activity	41:60	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	6	41	theme	polysaccharides	1015:1029	arg1	3.235 × 103 Da					1052:1065	3.235 × 103 Da	1052:1065	3.235 × 103 Da	1052:1065	The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively.					
33731198	6	41	theme	polysaccharides	1015:1029	arg1	weight					983:988	The molecular weight	969:988	The molecular weight of the two water-soluble polysaccharides	969:1029	The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively.					
33731198	7	42	theme	derivatization	1124:1137	arg1	analysis					1139:1146	PMP derivatization analysis	1120:1146	PMP derivatization analysis	1120:1146	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	4	43	theme	Sephadex	702:709	arg1	column					717:722	Sephadex G-100 column	702:722	Sephadex G-100 column	702:722	The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6.					
33731198	9	44	theme	reducing	1549:1556	arg1	power					1558:1562	weak total reducing power	1538:1562	weak total reducing power	1538:1562	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	6	45	theme	water-soluble	1001:1013	arg1	polysaccharides					1015:1029	the two water-soluble polysaccharides	993:1029	the two water-soluble polysaccharides	993:1029	The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively.					
33731198	10	46	dep	CONCLUSIONS	1650:1660	arg1	outcome					1666:1672	The outcome	1662:1672	CONCLUSIONS The outcome of the study	1650:1685	CONCLUSIONS The outcome of the study indicated that LBPs had good potential as medicine and food.					
33731198	8	47	from	glucose	1377:1383	arg1	ratio					1397:1401	a molar ratio	1389:1401	a molar ratio of 0.04:0.17:0.79	1389:1419	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	4	48	theme	DEAE	673:676	arg1	column					691:696	DEAE cellulose-52 column	673:696	DEAE cellulose-52 column	673:696	The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6.					
33731198	7	49	theme	glucuronic	1219:1228	arg1	acid					1230:1233	glucuronic acid	1219:1233	glucuronic acid	1219:1233	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	10	50	theme	good	1711:1714	arg1	potential					1716:1724	good potential	1711:1724	good potential	1711:1724	CONCLUSIONS The outcome of the study indicated that LBPs had good potential as medicine and food.					
33731198	5	51	with	β-pyranose	912:921	arg1	peaks					943:947	characteristic peaks	928:947	characteristic peaks of polysaccharides	928:966	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	9	52	dep	demonstrated	1473:1484	arg1	stronger					1601:1608	stronger	1601:1608	stronger	1601:1608	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	8	53	theme	LBPs-6	1335:1340	arg1	compositions					1319:1330	The monosaccharide compositions	1300:1330	The monosaccharide compositions of LBPs-6	1300:1340	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	8	53	theme	LBPs-6	1335:1340	arg1	mannose					1347:1353	mannose	1347:1353	mannose	1347:1353	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	9	54	theme	scavenging	1613:1622	arg1	rate					1624:1627	scavenging rate	1613:1627	scavenging rate	1613:1627	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	1	55	theme	Fungal	134:139	arg1	polysaccharides					141:155	BACKGROUND Fungal polysaccharides	123:155	BACKGROUND Fungal polysaccharides	123:155	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	7	56	from	glucose	1236:1242	arg1	ratio					1270:1274	a molar ratio	1262:1274	a molar ratio of 0.05:0.15:0.76:0.04	1262:1297	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	7	57	from	mannose	1210:1216	arg1	ratio					1270:1274	a molar ratio	1262:1274	a molar ratio of 0.05:0.15:0.76:0.04	1262:1297	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	7	58	theme	PMP	1120:1122	arg1	analysis					1139:1146	PMP derivatization analysis	1120:1146	PMP derivatization analysis	1120:1146	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	1	59	contain	have	234:237	arg2	structure					278:286	structure	278:286	structure	278:286	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	1	59	contain	have	234:237	arg1	polysaccharides					141:155	BACKGROUND Fungal polysaccharides	123:155	BACKGROUND Fungal polysaccharides	123:155	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	1	59	contain	have	234:237	arg2	composition					262:272	complex monosaccharide composition	239:272	complex monosaccharide composition	239:272	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	1	60	from	nature	222:227	arg1	class					184:188	a very important class	167:188	a very important class of biological macromolecules in nature	167:227	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	3	61	theme	antioxidant	530:540	arg1	activity					542:549	antioxidant activity	530:549	antioxidant activity	530:549	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	9	62	theme	water-soluble	1443:1455	arg1	polysaccharides					1457:1471	the two water-soluble polysaccharides	1435:1471	the two water-soluble polysaccharides	1435:1471	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	7	63	from	acid	1230:1233	arg1	ratio					1270:1274	a molar ratio	1262:1274	a molar ratio of 0.05:0.15:0.76:0.04	1262:1297	HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04.					
33731198	3	64	theme	structural	499:508	arg1	characteristics					510:524	the structural characteristics	495:524	the structural characteristics	495:524	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	1	65	theme	complex	239:245	arg1	composition					262:272	complex monosaccharide composition	239:272	complex monosaccharide composition	239:272	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	0	66	from	betulina	113:120	arg1	polysaccharides					83:97	two water-soluble polysaccharides	65:97	two water-soluble polysaccharides from Lenzites betulina	65:120	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	0	66	from	betulina	113:120	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	0	66	from	betulina	113:120	arg1	characteristics					21:35	structure characteristics	11:35	structure characteristics	11:35	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	0	66	from	betulina	113:120	arg1	activity					53:60	antioxidant activity	41:60	antioxidant activity	41:60	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	8	67	theme	0.04:0.17:0.79	1406:1419	arg1	ratio					1397:1401	a molar ratio	1389:1401	a molar ratio of 0.04:0.17:0.79	1389:1419	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	1	68	from	macromolecules	204:217	arg1	nature					222:227	nature	222:227	nature	222:227	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	4	69	theme	refined	755:761	arg1	polysaccharides					763:777	the two refined polysaccharides	747:777	the two refined polysaccharides	747:777	The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6.					
33731198	1	70	theme	monosaccharide	247:260	arg1	composition					262:272	complex monosaccharide composition	239:272	complex monosaccharide composition	239:272	BACKGROUND Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure.					
33731198	0	71	theme	water-soluble	69:81	arg1	polysaccharides					83:97	two water-soluble polysaccharides	65:97	two water-soluble polysaccharides from Lenzites betulina	65:120	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	8	72	theme	glucuronic	1356:1365	arg1	acid					1367:1370	glucuronic acid	1356:1370	glucuronic acid	1356:1370	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	3	73	dep	understand	484:493	arg1	to					481:482	to	481:482	to	481:482	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	74	theme	polysaccharides	554:568	arg1	characteristics					510:524	the structural characteristics	495:524	the structural characteristics	495:524	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	3	74	theme	polysaccharides	554:568	arg1	activity					542:549	antioxidant activity	530:549	antioxidant activity	530:549	RESULTS This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs).					
33731198	5	75	theme	typical	904:910	arg1	LBPs-6					893:898	LBPs-6	893:898	LBPs-6	893:898	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	5	75	theme	typical	904:910	arg1	β-pyranose					912:921	typical β-pyranose	904:921	typical β-pyranose with characteristic peaks of polysaccharides	904:966	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	5	75	theme	typical	904:910	arg1	LBPs-5					882:887	LBPs-5	882:887	LBPs-5	882:887	Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides.					
33731198	9	76	theme	strong	1486:1491	arg1	effects					1504:1510	strong scavenging effects	1486:1510	strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power	1486:1562	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33731198	8	77	theme	monosaccharide	1304:1317	arg1	compositions					1319:1330	The monosaccharide compositions	1300:1330	The monosaccharide compositions of LBPs-6	1300:1340	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	8	77	theme	monosaccharide	1304:1317	arg1	mannose					1347:1353	mannose	1347:1353	mannose	1347:1353	The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79.					
33731198	0	78	from	characteristics	21:35	arg1	betulina					113:120	betulina	113:120	betulina	113:120	Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina.					
33731198	4	79	dep	column	691:696	arg1	chromatographies					724:739	chromatographies	724:739	chromatographies	724:739	The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6.					
33731198	2	80	theme	hotspots	398:405	arg1	one					378:380	one	378:380	one	378:380	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	2	80	theme	hotspots	398:405	arg1	hotspots					398:405	the research hotspots	385:405	the research hotspots of scholars	385:417	These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad.					
33731198	9	81	theme	scavenging	1493:1502	arg1	effects					1504:1510	strong scavenging effects	1486:1510	strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power	1486:1562	Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5.					
33472179	1	0	theme	current	347:353	arg1	interest					355:362	great current interest	341:362	great current interest	341:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	6	1	from	homogenates	921:931	arg1	dCas9					893:897	extract dCas9	885:897	extract dCas9 from transfected cell homogenates	885:931	Finally, the imprinted nanoparticles were used to extract dCas9 from transfected cell homogenates.					
33472179	1	2	theme	short	188:192	arg1	CRISPR					215:220	CRISPR	215:220	CRISPR	215:220	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	2	theme	short	188:192	arg1	repeats					206:212	short palindromic repeats	188:212	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	3	theme	powerful	272:279	arg1	method					281:286	a powerful method	270:286	a powerful method for genetic modification (and regulation) that is of great current interest	270:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	3	theme	powerful	272:279	arg1	technology					256:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	6	4	used	used	877:880	arg2	nanoparticles					858:870	the imprinted nanoparticles	844:870	the imprinted nanoparticles	844:870	Finally, the imprinted nanoparticles were used to extract dCas9 from transfected cell homogenates.					
33472179	6	5	theme	extract	885:891	arg1	dCas9					893:897	extract dCas9	885:897	extract dCas9 from transfected cell homogenates	885:931	Finally, the imprinted nanoparticles were used to extract dCas9 from transfected cell homogenates.					
33472179	1	6	theme	palindromic	194:204	arg1	CRISPR					215:220	CRISPR	215:220	CRISPR	215:220	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	6	theme	palindromic	194:204	arg1	repeats					206:212	short palindromic repeats	188:212	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	0	7	theme	proteins	119:126	arg1	extraction					91:100	the extraction	87:100	the extraction of CRISPR/dCas9a proteins from transfected cells	87:149	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	0	8	theme	CRISPR/dCas9a	105:117	arg1	proteins					119:126	CRISPR/dCas9a proteins	105:126	CRISPR/dCas9a proteins from transfected cells	105:149	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	4	9	theme	HEK293T	745:751	arg1	cells					754:758	human embryonic kidney (HEK293T) cells	721:758	human embryonic kidney (HEK293T) cells	721:758	dCas9 was encoded in a plasmid which was then transfected into human embryonic kidney (HEK293T) cells.					
33472179	3	10	theme	molecular	535:543	arg1	imprinting					545:554	molecular imprinting	535:554	molecular imprinting	535:554	In this work, we employed molecular imprinting, using two peptides from the Cas9 protein, to make magnetic peptide-imprinted chitosan nanoparticles.					
33472179	3	11	from	protein	590:596	arg1	peptides					567:574	two peptides	563:574	two peptides from the Cas9 protein	563:596	In this work, we employed molecular imprinting, using two peptides from the Cas9 protein, to make magnetic peptide-imprinted chitosan nanoparticles.					
33472179	1	12	theme	genetic	292:298	arg1	modification					300:311	genetic modification	292:311	genetic modification	292:311	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	0	13	theme	transfected	133:143	arg1	cells					145:149	transfected cells	133:149	transfected cells	133:149	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	5	14	theme	total	815:819	arg1	kits					829:832	total protein kits	815:832	total protein kits	815:832	The expression of dCas9 protein was measured by using total protein kits.					
33472179	2	15	dep	new	384:386	arg1	economical					389:398	economical	389:398	economical	389:398	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	1	16	theme	repeats	206:212	arg1	method					281:286	a powerful method	270:286	a powerful method for genetic modification (and regulation) that is of great current interest	270:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	16	theme	repeats	206:212	arg1	technology					256:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	5	17	theme	dCas9	779:783	arg1	protein					785:791	dCas9 protein	779:791	dCas9 protein	779:791	The expression of dCas9 protein was measured by using total protein kits.					
33472179	2	18	theme	methods	400:406	arg1	important					490:498	important	490:498	important	490:498	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	2	18	theme	methods	400:406	arg1	development					369:379	The development	365:379	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells	365:477	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	2	18	theme	methods	400:406	arg1	advance					500:506	advance	500:506	advance	500:506	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	5	19	theme	protein	785:791	arg1	expression					765:774	The expression	761:774	The expression of dCas9 protein	761:791	The expression of dCas9 protein was measured by using total protein kits.					
33472179	6	20	theme	imprinted	848:856	arg1	nanoparticles					858:870	the imprinted nanoparticles	844:870	the imprinted nanoparticles	844:870	Finally, the imprinted nanoparticles were used to extract dCas9 from transfected cell homogenates.					
33472179	4	21	theme	embryonic	727:735	arg1	cells					754:758	human embryonic kidney (HEK293T) cells	721:758	human embryonic kidney (HEK293T) cells	721:758	dCas9 was encoded in a plasmid which was then transfected into human embryonic kidney (HEK293T) cells.					
33472179	0	22	theme	Epitope	0:6	arg1	recognition					8:18	Epitope recognition	0:18	Epitope recognition of magnetic peptide-imprinted chitosan composite	0:67	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	4	23	theme	human	721:725	arg1	cells					754:758	human embryonic kidney (HEK293T) cells	721:758	human embryonic kidney (HEK293T) cells	721:758	dCas9 was encoded in a plasmid which was then transfected into human embryonic kidney (HEK293T) cells.					
33472179	0	24	theme	magnetic	23:30	arg1	composite					59:67	magnetic peptide-imprinted chitosan composite	23:67	magnetic peptide-imprinted chitosan composite	23:67	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	1	25	theme	/CRISPR-associated	222:239	arg1	method					281:286	a powerful method	270:286	a powerful method for genetic modification (and regulation) that is of great current interest	270:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	25	theme	/CRISPR-associated	222:239	arg1	technology					256:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	3	26	theme	chitosan	634:641	arg1	nanoparticles					643:655	magnetic peptide-imprinted chitosan nanoparticles	607:655	magnetic peptide-imprinted chitosan nanoparticles	607:655	In this work, we employed molecular imprinting, using two peptides from the Cas9 protein, to make magnetic peptide-imprinted chitosan nanoparticles.					
33472179	4	27	theme	kidney	737:742	arg1	cells					754:758	human embryonic kidney (HEK293T) cells	721:758	human embryonic kidney (HEK293T) cells	721:758	dCas9 was encoded in a plasmid which was then transfected into human embryonic kidney (HEK293T) cells.					
33472179	1	28	theme	protein	241:247	arg1	method					281:286	a powerful method	270:286	a powerful method for genetic modification (and regulation) that is of great current interest	270:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	28	theme	protein	241:247	arg1	technology					256:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	0	29	theme	chitosan	50:57	arg1	composite					59:67	magnetic peptide-imprinted chitosan composite	23:67	magnetic peptide-imprinted chitosan composite	23:67	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	0	30	from	extraction	91:100	arg1	cells					145:149	transfected cells	133:149	transfected cells	133:149	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	5	31	theme	protein	821:827	arg1	kits					829:832	total protein kits	815:832	total protein kits	815:832	The expression of dCas9 protein was measured by using total protein kits.					
33472179	0	32	theme	peptide-imprinted	32:48	arg1	composite					59:67	magnetic peptide-imprinted chitosan composite	23:67	magnetic peptide-imprinted chitosan composite	23:67	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	3	33	theme	magnetic	607:614	arg1	nanoparticles					643:655	magnetic peptide-imprinted chitosan nanoparticles	607:655	magnetic peptide-imprinted chitosan nanoparticles	607:655	In this work, we employed molecular imprinting, using two peptides from the Cas9 protein, to make magnetic peptide-imprinted chitosan nanoparticles.					
33472179	3	34	theme	Cas9	585:588	arg1	protein					590:596	the Cas9 protein	581:596	the Cas9 protein	581:596	In this work, we employed molecular imprinting, using two peptides from the Cas9 protein, to make magnetic peptide-imprinted chitosan nanoparticles.					
33472179	1	35	theme	Cas9	250:253	arg1	method					281:286	a powerful method	270:286	a powerful method for genetic modification (and regulation) that is of great current interest	270:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	35	theme	Cas9	250:253	arg1	technology					256:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	2	36	theme	transfected	461:471	arg1	cells					473:477	transfected cells	461:477	transfected cells	461:477	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	3	37	theme	peptide-imprinted	616:632	arg1	nanoparticles					643:655	magnetic peptide-imprinted chitosan nanoparticles	607:655	magnetic peptide-imprinted chitosan nanoparticles	607:655	In this work, we employed molecular imprinting, using two peptides from the Cas9 protein, to make magnetic peptide-imprinted chitosan nanoparticles.					
33472179	0	38	theme	composite	59:67	arg1	recognition					8:18	Epitope recognition	0:18	Epitope recognition of magnetic peptide-imprinted chitosan composite	0:67	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	6	39	theme	cell	916:919	arg1	homogenates					921:931	transfected cell homogenates	904:931	transfected cell homogenates	904:931	Finally, the imprinted nanoparticles were used to extract dCas9 from transfected cell homogenates.					
33472179	6	40	theme	transfected	904:914	arg1	homogenates					921:931	transfected cell homogenates	904:931	transfected cell homogenates	904:931	Finally, the imprinted nanoparticles were used to extract dCas9 from transfected cell homogenates.					
33472179	2	41	dep	important	490:498	arg1	important					490:498	important	490:498	important	490:498	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	2	41	dep	important	490:498	arg1	development					369:379	The development	365:379	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells	365:477	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	2	41	dep	important	490:498	arg1	advance					500:506	advance	500:506	advance	500:506	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	1	42	theme	clustered	156:164	arg1	method					281:286	a powerful method	270:286	a powerful method for genetic modification (and regulation) that is of great current interest	270:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	42	theme	clustered	156:164	arg1	technology					256:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	2	43	theme	new	384:386	arg1	methods					400:406	new, economical methods	384:406	new, economical methods	384:406	The development of new, economical methods of detecting and extracting Cas9 (and/or dCas9) from transfected cells is thus an important advance.					
33472179	1	44	theme	great	341:345	arg1	interest					355:362	great current interest	341:362	great current interest	341:362	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	45	theme	interspaced	176:186	arg1	CRISPR					215:220	CRISPR	215:220	CRISPR	215:220	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	1	45	theme	interspaced	176:186	arg1	repeats					206:212	short palindromic repeats	188:212	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology	152:265	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology is a powerful method for genetic modification (and regulation) that is of great current interest.					
33472179	0	46	from	cells	145:149	arg1	extraction					91:100	the extraction	87:100	the extraction of CRISPR/dCas9a proteins from transfected cells	87:149	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33472179	0	46	from	cells	145:149	arg1	proteins					119:126	CRISPR/dCas9a proteins	105:126	CRISPR/dCas9a proteins from transfected cells	105:149	Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.					
33768618	0	0	theme	concentration	58:70	arg1	effect					4:9	The effect	0:9	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability	0:92	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	0	1	from	effect	4:9	arg1	stability					84:92	oxytocin stability	75:92	oxytocin stability	75:92	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	5	2	theme	citrate/phosphate	616:632	arg1	buffer					634:639	citrate/phosphate buffer	616:639	citrate/phosphate buffer for the oxytocin stability	616:666	Acetate buffer was found to work better than citrate/phosphate buffer for the oxytocin stability.					
33768618	3	3	from	stability	464:472	arg1	solution					477:484	solution	477:484	solution	477:484	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	4	4	theme	concentration	539:551	arg1	effect					491:496	The effect	487:496	The effect of buffer composition and acetate buffer concentration	487:551	The effect of buffer composition and acetate buffer concentration was also studied.					
33768618	8	5	theme	oxytocin	1158:1165	arg1	stability					1167:1175	oxytocin stability	1158:1175	oxytocin stability	1158:1175	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	7	6	theme	negligible	938:947	arg1	effect					949:954	negligible effect	938:954	negligible effect	938:954	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	5	7	theme	Acetate	571:577	arg1	buffer					579:584	Acetate buffer	571:584	Acetate buffer	571:584	Acetate buffer was found to work better than citrate/phosphate buffer for the oxytocin stability.					
33768618	2	8	from	climates	294:301	arg1	problematic					266:276	problematic	266:276	problematic	266:276	Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates.					
33768618	0	9	theme	oxytocin	75:82	arg1	stability					84:92	oxytocin stability	75:92	oxytocin stability	75:92	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	3	10	theme	study	320:324	arg1	aim					308:310	The aim	304:310	The aim of this study	304:324	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	11	from	effect	349:354	arg1	stability					464:472	oxytocin stability	455:472	oxytocin stability in solution	455:484	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	8	12	dep	effect	1148:1153	arg1	to					1134:1135	to	1134:1135	to	1134:1135	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	6	13	theme	buffer	683:688	arg1	0.025 M					706:712	0.025 M or less	706:720	0.025 M or less	706:720	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	6	13	theme	buffer	683:688	arg1	concentrations					690:703	Lower acetate buffer concentrations	669:703	Lower acetate buffer concentrations (0.025 M or less)	669:721	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	9	14	theme	various	1316:1322	arg1	proteins					1348:1355	proteins	1348:1355	proteins	1348:1355	These results were surprising given the present literature on trehalose as a stabilizer for various biomolecules, including proteins and lipids.					
33768618	9	14	theme	various	1316:1322	arg1	lipids					1361:1366	lipids	1361:1366	lipids	1361:1366	These results were surprising given the present literature on trehalose as a stabilizer for various biomolecules, including proteins and lipids.					
33768618	9	14	theme	various	1316:1322	arg1	biomolecules					1324:1335	various biomolecules	1316:1335	various biomolecules	1316:1335	These results were surprising given the present literature on trehalose as a stabilizer for various biomolecules, including proteins and lipids.					
33768618	0	15	theme	antioxidants	25:36	arg1	effect					4:9	The effect	0:9	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability	0:92	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	6	16	theme	oxytocin	757:764	arg1	stability					766:774	improved oxytocin stability	748:774	improved oxytocin stability	748:774	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	2	17	theme	formulations	246:257	arg1	transport					224:232	transport	224:232	transport	224:232	Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates.					
33768618	2	17	theme	formulations	246:257	arg1	storage					212:218	storage	212:218	storage	212:218	Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates.					
33768618	6	18	theme	acetate	675:681	arg1	0.025 M					706:712	0.025 M or less	706:720	0.025 M or less	706:720	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	6	18	theme	acetate	675:681	arg1	concentrations					690:703	Lower acetate buffer concentrations	669:703	Lower acetate buffer concentrations (0.025 M or less)	669:721	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	3	19	theme	butylated	405:413	arg1	antioxidants					380:391	select antioxidants	373:391	select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid)	373:450	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	19	theme	butylated	405:413	arg1	hydroxytoluene					415:428	butylated hydroxytoluene	405:428	butylated hydroxytoluene	405:428	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	8	20	theme	negligible	1137:1146	arg1	effect					1148:1153	small to negligible effect	1128:1153	small to negligible effect	1128:1153	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	3	21	dep	antioxidants	380:391	arg1	acid					446:449	l-ascorbic acid	435:449	l-ascorbic acid	435:449	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	21	dep	antioxidants	380:391	arg1	antioxidants					380:391	select antioxidants	373:391	select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid)	373:450	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	21	dep	antioxidants	380:391	arg1	acid					399:402	uric acid	394:402	uric acid	394:402	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	21	dep	antioxidants	380:391	arg1	hydroxytoluene					415:428	butylated hydroxytoluene	405:428	butylated hydroxytoluene	405:428	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	6	22	theme	improved	748:755	arg1	stability					766:774	improved oxytocin stability	748:774	improved oxytocin stability	748:774	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	6	23	theme	Lower	669:673	arg1	0.025 M					706:712	0.025 M or less	706:720	0.025 M or less	706:720	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	6	23	theme	Lower	669:673	arg1	concentrations					690:703	Lower acetate buffer concentrations	669:703	Lower acetate buffer concentrations (0.025 M or less)	669:721	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	3	24	theme	uric	394:397	arg1	antioxidants					380:391	select antioxidants	373:391	select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid)	373:450	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	24	theme	uric	394:397	arg1	acid					399:402	uric acid	394:402	uric acid	394:402	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	25	theme	trehalose	359:367	arg1	effect					349:354	the effect	345:354	the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution	345:484	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	8	26	theme	acetate	1208:1214	arg1	buffer					1216:1221	acetate buffer	1208:1221	acetate buffer	1208:1221	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	7	27	theme	butylated	909:917	arg1	hydroxytoluene					919:932	butylated hydroxytoluene	909:932	butylated hydroxytoluene	909:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	27	theme	butylated	909:917	arg1	antioxidants					882:893	the antioxidants uric acid and butylated hydroxytoluene	878:932	the antioxidants uric acid and butylated hydroxytoluene	878:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	0	28	theme	trehalose	14:22	arg1	effect					4:9	The effect	0:9	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability	0:92	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	4	29	theme	composition	508:518	arg1	effect					491:496	The effect	487:496	The effect of buffer composition and acetate buffer concentration	487:551	The effect of buffer composition and acetate buffer concentration was also studied.					
33768618	2	30	theme	hot/tropical	281:292	arg1	climates					294:301	hot/tropical climates	281:301	hot/tropical climates	281:301	Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates.					
33768618	6	31	theme	higher	788:793	arg1	concentrations					795:808	higher concentrations	788:808	higher concentrations	788:808	Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations.					
33768618	7	32	contain	had	934:936	arg1	acid					900:903	uric acid	895:903	uric acid	895:903	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	32	contain	had	934:936	arg2	effect					949:954	negligible effect	938:954	negligible effect	938:954	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	32	contain	had	934:936	arg1	antioxidants					882:893	the antioxidants uric acid and butylated hydroxytoluene	878:932	the antioxidants uric acid and butylated hydroxytoluene	878:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	32	contain	had	934:936	arg1	hydroxytoluene					919:932	butylated hydroxytoluene	909:932	butylated hydroxytoluene	909:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	4	33	theme	buffer	532:537	arg1	concentration					539:551	acetate buffer concentration	524:551	acetate buffer concentration	524:551	The effect of buffer composition and acetate buffer concentration was also studied.					
33768618	8	34	theme	great	1081:1085	arg1	stabilizer					1087:1096	a great stabilizer	1079:1096	a great stabilizer for biomolecules	1079:1113	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	8	35	contain	had	1124:1126	arg1	it					1116:1117	it	1116:1117	it	1116:1117	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	8	35	contain	had	1124:1126	arg2	effect					1148:1153	small to negligible effect	1128:1153	small to negligible effect	1128:1153	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	3	36	theme	l-ascorbic	435:444	arg1	acid					446:449	l-ascorbic acid	435:449	l-ascorbic acid	435:449	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	36	theme	l-ascorbic	435:444	arg1	antioxidants					380:391	select antioxidants	373:391	select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid)	373:450	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	37	theme	antioxidants	380:391	arg1	effect					349:354	the effect	345:354	the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution	345:484	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	4	38	theme	acetate	524:530	arg1	concentration					539:551	acetate buffer concentration	524:551	acetate buffer concentration	524:551	The effect of buffer composition and acetate buffer concentration was also studied.					
33768618	7	39	dep	antioxidants	882:893	arg1	acid					900:903	uric acid	895:903	uric acid	895:903	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	39	dep	antioxidants	882:893	arg1	hydroxytoluene					919:932	butylated hydroxytoluene	909:932	butylated hydroxytoluene	909:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	39	dep	antioxidants	882:893	arg1	antioxidants					882:893	the antioxidants uric acid and butylated hydroxytoluene	878:932	the antioxidants uric acid and butylated hydroxytoluene	878:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	8	40	dep	1 M	1201:1203	arg1	up					1195:1196	up	1195:1196	up	1195:1196	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	8	41	theme	small	1128:1132	arg1	effect					1148:1153	small to negligible effect	1128:1153	small to negligible effect	1128:1153	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	7	42	theme	faster	1025:1030	arg1	degradation					1032:1042	significantly faster degradation	1011:1042	significantly faster degradation	1011:1042	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	3	43	theme	select	373:378	arg1	acid					446:449	l-ascorbic acid	435:449	l-ascorbic acid	435:449	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	43	theme	select	373:378	arg1	antioxidants					380:391	select antioxidants	373:391	select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid)	373:450	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	43	theme	select	373:378	arg1	acid					399:402	uric acid	394:402	uric acid	394:402	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	3	43	theme	select	373:378	arg1	hydroxytoluene					415:428	butylated hydroxytoluene	405:428	butylated hydroxytoluene	405:428	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	4	44	theme	buffer	501:506	arg1	composition					508:518	buffer composition	501:518	buffer composition	501:518	The effect of buffer composition and acetate buffer concentration was also studied.					
33768618	5	45	theme	oxytocin	649:656	arg1	stability					658:666	the oxytocin stability	645:666	the oxytocin stability	645:666	Acetate buffer was found to work better than citrate/phosphate buffer for the oxytocin stability.					
33768618	3	46	theme	oxytocin	455:462	arg1	stability					464:472	oxytocin stability	455:472	oxytocin stability in solution	455:484	The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution.					
33768618	2	47	theme	oxytocin	237:244	arg1	formulations					246:257	oxytocin formulations	237:257	oxytocin formulations	237:257	Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates.					
33768618	7	48	theme	oxytocin	963:970	arg1	stability					972:980	the oxytocin stability	959:980	the oxytocin stability	959:980	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	1	49	theme	cyclic	109:114	arg1	nonapeptide					116:126	a cyclic nonapeptide	107:126	a cyclic nonapeptide used to induce labor and prevent bleeding after childbirth	107:185	Oxytocin is a cyclic nonapeptide used to induce labor and prevent bleeding after childbirth.					
33768618	1	49	theme	cyclic	109:114	arg1	Oxytocin					95:102	Oxytocin	95:102	Oxytocin	95:102	Oxytocin is a cyclic nonapeptide used to induce labor and prevent bleeding after childbirth.					
33768618	9	50	theme	present	1264:1270	arg1	literature					1272:1281	the present literature	1260:1281	the present literature on trehalose	1260:1294	These results were surprising given the present literature on trehalose as a stabilizer for various biomolecules, including proteins and lipids.					
33768618	7	51	theme	oxytocin	850:857	arg1	pathways					838:845	known degradation pathways	820:845	known degradation pathways of oxytocin	820:857	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	52	theme	l-ascorbic	988:997	arg1	acid					999:1002	l-ascorbic acid	988:1002	l-ascorbic acid	988:1002	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	53	theme	known	820:824	arg1	pathways					838:845	known degradation pathways	820:845	known degradation pathways of oxytocin	820:857	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	2	54	from	problematic	266:276	arg1	climates					294:301	hot/tropical climates	281:301	hot/tropical climates	281:301	Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates.					
33768618	7	55	theme	degradation	826:836	arg1	pathways					838:845	known degradation pathways	820:845	known degradation pathways of oxytocin	820:857	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	0	56	theme	buffer	51:56	arg1	concentration					58:70	acetate buffer concentration	43:70	acetate buffer concentration	43:70	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	7	57	theme	uric	895:898	arg1	acid					900:903	uric acid	895:903	uric acid	895:903	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	7	57	theme	uric	895:898	arg1	antioxidants					882:893	the antioxidants uric acid and butylated hydroxytoluene	878:932	the antioxidants uric acid and butylated hydroxytoluene	878:932	Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation.					
33768618	0	58	theme	acetate	43:49	arg1	concentration					58:70	acetate buffer concentration	43:70	acetate buffer concentration	43:70	The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability.					
33768618	8	59	from	1 M	1201:1203	arg1	buffer					1216:1221	acetate buffer	1208:1221	acetate buffer	1208:1221	Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer.					
33768618	9	60	from	literature	1272:1281	arg1	trehalose					1286:1294	trehalose	1286:1294	trehalose	1286:1294	These results were surprising given the present literature on trehalose as a stabilizer for various biomolecules, including proteins and lipids.					
32739385	4	0	theme	light	519:523	arg1	scattering					525:534	Dynamic light scattering	511:534	Dynamic light scattering	511:534	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	4	1	used	used	583:586	arg2	techniques					567:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	8	2	theme	BLE	1111:1113	arg1	formulations					1126:1137	the BLE and HA-BLE formulations	1107:1137	formulations	1126:1137	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	1	3	theme	postoperative	251:263	arg1	pain					265:268	postoperative pain	251:268	postoperative pain	251:268	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	8	4	theme	bupivacaine	1077:1087	arg1	bupivacaine					1077:1087	bupivacaine	1077:1087	bupivacaine	1077:1087	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	8	4	theme	bupivacaine	1077:1087	arg1	%					1063:1063	Approximately 80%	1047:1063	Approximately 80%	1047:1063	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	8	4	theme	bupivacaine	1077:1087	arg1	%					1072:1072	100%	1069:1072	100% of bupivacaine	1069:1087	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	4	5	theme	rheological	537:547	arg1	techniques					567:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	10	6	theme	anesthetic	1396:1405	arg1	effect					1407:1412	anesthetic effect	1396:1412	anesthetic effect	1396:1412	Results indicate that incorporating the BLEs into the hydrogel significantly increased anesthetic effect by protecting the BLE droplets from the in vivo environment.					
32739385	1	7	theme	research	163:170	arg1	purpose					147:153	The purpose	143:153	The purpose of this research	143:170	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	1	8	theme	pain	265:268	arg1	control					240:246	control	240:246	control of postoperative pain	240:268	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	0	9	theme	rat	112:114	arg1	model					136:140	a rat sciatic nerve block model	110:140	a rat sciatic nerve block model	110:140	Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.					
32739385	6	10	theme	droplet	833:839	arg1	size					841:844	droplet size	833:844	droplet size	833:844	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	5	11	theme	rat	624:626	arg1	block					642:646	A rat sciatic nerve block	622:646	A rat sciatic nerve block with a thermal nociceptive assay	622:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	7	12	theme	372 ± 23.7 Pa	1032:1044	arg1	modulus					1021:1027	an elastic modulus	1010:1027	an elastic modulus of 372 ± 23.7 Pa	1010:1044	The HA-BLE formulation could be injected through 25 g needles and had an elastic modulus of 372 ± 23.7 Pa.					
32739385	5	13	theme	sciatic	628:634	arg1	block					642:646	A rat sciatic nerve block	622:646	A rat sciatic nerve block with a thermal nociceptive assay	622:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	9	14	theme	anesthetic	1214:1223	arg1	area					1225:1228	a 5-times greater anesthetic area	1196:1228	a 5-times greater anesthetic area under the curve	1196:1244	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	0	15	theme	nerve	124:128	arg1	model					136:140	a rat sciatic nerve block model	110:140	a rat sciatic nerve block model	110:140	Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.					
32739385	6	16	contain	had	811:813	arg1	droplets					802:809	The BLE droplets	794:809	The BLE droplets	794:809	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	16	contain	had	811:813	arg2	index					866:870	polydispersity index	851:870	polydispersity index	851:870	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	16	contain	had	811:813	arg2	potential					822:830	a zeta potential	815:830	a zeta potential	815:830	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	16	contain	had	811:813	arg2	size					841:844	droplet size	833:844	droplet size	833:844	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	5	17	theme	nerve	636:640	arg1	block					642:646	A rat sciatic nerve block	622:646	A rat sciatic nerve block with a thermal nociceptive assay	622:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	2	18	theme	emulsion	296:303	arg1	droplets					311:318	Bupivacaine-loaded lipid emulsion (BLE) droplets	271:318	Bupivacaine-loaded lipid emulsion (BLE) droplets	271:318	Bupivacaine-loaded lipid emulsion (BLE) droplets were created by high-speed homogenization.					
32739385	0	19	theme	sciatic	116:122	arg1	model					136:140	a rat sciatic nerve block model	110:140	a rat sciatic nerve block model	110:140	Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.					
32739385	4	20	theme	Dynamic	511:517	arg1	scattering					525:534	Dynamic light scattering	511:534	Dynamic light scattering	511:534	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	6	21	theme	polydispersity	851:864	arg1	index					866:870	polydispersity index	851:870	polydispersity index	851:870	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	2	22	theme	lipid	290:294	arg1	BLE					306:308	BLE	306:308	BLE	306:308	Bupivacaine-loaded lipid emulsion (BLE) droplets were created by high-speed homogenization.					
32739385	2	22	theme	lipid	290:294	arg1	emulsion					296:303	Bupivacaine-loaded lipid emulsion	271:303	Bupivacaine-loaded lipid emulsion (BLE) droplets	271:318	Bupivacaine-loaded lipid emulsion (BLE) droplets were created by high-speed homogenization.					
32739385	3	23	theme	injectable	464:473	arg1	HA-BLE					502:507	HA-BLE	502:507	HA-BLE	502:507	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	3	23	theme	injectable	464:473	arg1	formulation					489:499	an injectable composite gel formulation	461:499	an injectable composite gel formulation (HA-BLE)	461:508	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	0	24	theme	bupivacaine-loaded	11:28	arg1	emulsions					30:38	bupivacaine-loaded emulsions	11:38	bupivacaine-loaded emulsions	11:38	Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.					
32739385	10	25	theme	BLE	1432:1434	arg1	droplets					1436:1443	the BLE droplets	1428:1443	the BLE droplets	1428:1443	Results indicate that incorporating the BLEs into the hydrogel significantly increased anesthetic effect by protecting the BLE droplets from the in vivo environment.					
32739385	10	26	dep	in	1454:1455	arg1	vivo					1457:1460	vivo	1457:1460	vivo	1457:1460	Results indicate that incorporating the BLEs into the hydrogel significantly increased anesthetic effect by protecting the BLE droplets from the in vivo environment.					
32739385	5	27	theme	thermal	655:661	arg1	assay					675:679	a thermal nociceptive assay	653:679	a thermal nociceptive assay	653:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	6	28	theme	BLE	798:800	arg1	droplets					802:809	The BLE droplets	794:809	The BLE droplets	794:809	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	9	29	theme	greater	1206:1212	arg1	area					1225:1228	a 5-times greater anesthetic area	1196:1228	a 5-times greater anesthetic area under the curve	1196:1244	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	7	30	theme	HA-BLE	944:949	arg1	formulation					951:961	The HA-BLE formulation	940:961	The HA-BLE formulation	940:961	The HA-BLE formulation could be injected through 25 g needles and had an elastic modulus of 372 ± 23.7 Pa.					
32739385	9	31	theme	HA-BLE	1173:1178	arg1	formulation					1180:1190	The HA-BLE formulation	1169:1190	The HA-BLE formulation	1169:1190	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	10	32	theme	in	1454:1455	arg1	environment					1462:1472	the in vivo environment	1450:1472	the in vivo environment	1450:1472	Results indicate that incorporating the BLEs into the hydrogel significantly increased anesthetic effect by protecting the BLE droplets from the in vivo environment.					
32739385	1	33	theme	novel	190:194	arg1	system					229:234	a novel long-acting bupivacaine delivery system	188:234	a novel long-acting bupivacaine delivery system for control of postoperative pain	188:268	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	0	34	theme	block	130:134	arg1	model					136:140	a rat sciatic nerve block model	110:140	a rat sciatic nerve block model	110:140	Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.					
32739385	6	35	theme	-40.8 ± 0.66 mV	875:889	arg1	index					866:870	polydispersity index	851:870	polydispersity index	851:870	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	35	theme	-40.8 ± 0.66 mV	875:889	arg1	potential					822:830	a zeta potential	815:830	a zeta potential	815:830	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	35	theme	-40.8 ± 0.66 mV	875:889	arg1	size					841:844	droplet size	833:844	droplet size	833:844	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	1	36	theme	long-acting	196:206	arg1	system					229:234	a novel long-acting bupivacaine delivery system	188:234	a novel long-acting bupivacaine delivery system for control of postoperative pain	188:268	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	3	37	theme	hydrogel	435:442	arg1	system					444:449	a crosslinked-hyaluronic acid hydrogel system	405:449	a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE)	405:508	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	3	38	theme	composite	475:483	arg1	HA-BLE					502:507	HA-BLE	502:507	HA-BLE	502:507	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	3	38	theme	composite	475:483	arg1	formulation					489:499	an injectable composite gel formulation	461:499	an injectable composite gel formulation (HA-BLE)	461:508	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	1	39	theme	bupivacaine	208:218	arg1	system					229:234	a novel long-acting bupivacaine delivery system	188:234	a novel long-acting bupivacaine delivery system for control of postoperative pain	188:268	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	1	40	theme	delivery	220:227	arg1	system					229:234	a novel long-acting bupivacaine delivery system	188:234	a novel long-acting bupivacaine delivery system for control of postoperative pain	188:268	The purpose of this research was to evaluate a novel long-acting bupivacaine delivery system for control of postoperative pain.					
32739385	3	41	theme	crosslinked-hyaluronic	407:428	arg1	system					444:449	a crosslinked-hyaluronic acid hydrogel system	405:449	a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE)	405:508	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	5	42	used	used	685:688	arg2	block					642:646	A rat sciatic nerve block	622:646	A rat sciatic nerve block with a thermal nociceptive assay	622:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	6	43	theme	zeta	817:820	arg1	potential					822:830	a zeta potential	815:830	a zeta potential	815:830	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	5	44	theme	liposomal	771:779	arg1	controls					741:748	controls	741:748	controls	741:748	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	5	44	theme	liposomal	771:779	arg1	bupivacaine					781:791	liposomal bupivacaine	771:791	liposomal bupivacaine	771:791	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	6	45	theme	299 ± 1.77 nm	892:904	arg1	index					866:870	polydispersity index	851:870	polydispersity index	851:870	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	45	theme	299 ± 1.77 nm	892:904	arg1	potential					822:830	a zeta potential	815:830	a zeta potential	815:830	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	45	theme	299 ± 1.77 nm	892:904	arg1	size					841:844	droplet size	833:844	droplet size	833:844	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	3	46	theme	acid	430:433	arg1	system					444:449	a crosslinked-hyaluronic acid hydrogel system	405:449	a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE)	405:508	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	4	47	theme	release	559:565	arg1	techniques					567:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	3	48	theme	BLE	367:369	arg1	droplets					371:378	The BLE droplets	363:378	The BLE droplets	363:378	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	7	49	theme	elastic	1013:1019	arg1	modulus					1021:1027	an elastic modulus	1010:1027	an elastic modulus of 372 ± 23.7 Pa	1010:1044	The HA-BLE formulation could be injected through 25 g needles and had an elastic modulus of 372 ± 23.7 Pa.					
32739385	5	50	theme	nociceptive	663:673	arg1	assay					675:679	a thermal nociceptive assay	653:679	a thermal nociceptive assay	653:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	4	51	theme	drug	554:557	arg1	release					559:565	drug release	554:565	drug release	554:565	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	8	52	theme	HA-BLE	1119:1124	arg1	formulations					1126:1137	the BLE and HA-BLE formulations	1107:1137	formulations	1126:1137	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	2	53	theme	Bupivacaine-loaded	271:288	arg1	BLE					306:308	BLE	306:308	BLE	306:308	Bupivacaine-loaded lipid emulsion (BLE) droplets were created by high-speed homogenization.					
32739385	2	53	theme	Bupivacaine-loaded	271:288	arg1	emulsion					296:303	Bupivacaine-loaded lipid emulsion	271:303	Bupivacaine-loaded lipid emulsion (BLE) droplets	271:318	Bupivacaine-loaded lipid emulsion (BLE) droplets were created by high-speed homogenization.					
32739385	7	54	theme	25 g	989:992	arg1	needles					994:1000	25 g needles	989:1000	25 g needles	989:1000	The HA-BLE formulation could be injected through 25 g needles and had an elastic modulus of 372 ± 23.7 Pa.					
32739385	2	55	theme	high-speed	336:345	arg1	homogenization					347:360	high-speed homogenization	336:360	high-speed homogenization	336:360	Bupivacaine-loaded lipid emulsion (BLE) droplets were created by high-speed homogenization.					
32739385	5	56	theme	bupivacaine	751:761	arg1	controls					741:748	controls	741:748	controls	741:748	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	5	56	theme	bupivacaine	751:761	arg1	HCl					763:765	bupivacaine HCl	751:765	bupivacaine HCl	751:765	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	5	57	theme	anesthetic	706:715	arg1	effect					717:722	the anesthetic effect	702:722	the anesthetic effect	702:722	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	9	58	theme	anesthetic	1253:1262	arg1	long					1291:1294	long	1291:1294	long	1291:1294	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	9	58	theme	anesthetic	1253:1262	arg1	duration					1264:1271	an anesthetic duration	1250:1271	an anesthetic duration that was twice as long as controls	1250:1306	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	5	59	with	block	642:646	arg1	assay					675:679	a thermal nociceptive assay	653:679	a thermal nociceptive assay	653:679	A rat sciatic nerve block with a thermal nociceptive assay was used to evaluate the anesthetic effect in comparison to controls, bupivacaine HCl and liposomal bupivacaine.					
32739385	3	60	theme	gel	485:487	arg1	HA-BLE					502:507	HA-BLE	502:507	HA-BLE	502:507	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	3	60	theme	gel	485:487	arg1	formulation					489:499	an injectable composite gel formulation	461:499	an injectable composite gel formulation (HA-BLE)	461:508	The BLE droplets were then entrapped into a crosslinked-hyaluronic acid hydrogel system to create an injectable composite gel formulation (HA-BLE).					
32739385	8	61	attach	released	1093:1100	arg2	bupivacaine					1077:1087	bupivacaine	1077:1087	bupivacaine	1077:1087	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	8	61	attach	released	1093:1100	arg1	formulations					1126:1137	the BLE and HA-BLE formulations	1107:1137	formulations	1126:1137	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	8	61	attach	released	1093:1100	arg2	%					1072:1072	100%	1069:1072	100% of bupivacaine	1069:1087	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	8	61	attach	released	1093:1100	arg2	%					1063:1063	Approximately 80%	1047:1063	Approximately 80%	1047:1063	Approximately 80% and 100% of bupivacaine was released from the BLE and HA-BLE formulations by 20 and 68 h, respectively.					
32739385	6	62	theme	0.409 ± 0.037	911:923	arg1	index					866:870	polydispersity index	851:870	polydispersity index	851:870	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	62	theme	0.409 ± 0.037	911:923	arg1	potential					822:830	a zeta potential	815:830	a zeta potential	815:830	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	6	62	theme	0.409 ± 0.037	911:923	arg1	size					841:844	droplet size	833:844	droplet size	833:844	The BLE droplets had a zeta potential, droplet size, and polydispersity index of -40.8 ± 0.66 mV, 299 ± 1.77 nm, and 0.409 ± 0.037, respectively.					
32739385	0	63	theme	anesthetic	76:85	arg1	effect					87:92	anesthetic effect	76:92	anesthetic effect	76:92	Entrapping bupivacaine-loaded emulsions in a crosslinked-hydrogel increases anesthetic effect and duration in a rat sciatic nerve block model.					
32739385	4	64	theme	scattering	525:534	arg1	techniques					567:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques	511:576	Dynamic light scattering, rheological, and drug release techniques were used to characterize the formulations.					
32739385	9	65	contain	had	1192:1194	arg1	formulation					1180:1190	The HA-BLE formulation	1169:1190	The HA-BLE formulation	1169:1190	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	9	65	contain	had	1192:1194	arg2	duration					1264:1271	an anesthetic duration	1250:1271	an anesthetic duration that was twice as long as controls	1250:1306	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	9	65	contain	had	1192:1194	arg2	long					1291:1294	long	1291:1294	long	1291:1294	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
32739385	9	65	contain	had	1192:1194	arg2	area					1225:1228	a 5-times greater anesthetic area	1196:1228	a 5-times greater anesthetic area under the curve	1196:1244	The HA-BLE formulation had a 5-times greater anesthetic area under the curve and an anesthetic duration that was twice as long as controls.					
34333047	2	0	theme	[Yeast	476:481	arg1	2.5 g/L					491:497	agar [Yeast extract 2.5 g/L	471:497	agar [Yeast extract 2.5 g/L	471:497	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	7	1	theme	continuous	1448:1457	arg1	freezing					1466:1473	continuous -80 °C freezing	1448:1473	continuous -80 °C freezing	1448:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	7	2	theme	blood	1421:1425	arg1	requirements					1431:1442	requirements	1431:1442	requirements for continuous -80 °C freezing	1431:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	7	2	theme	blood	1421:1425	arg1	availability					1397:1408	availability	1397:1408	availability of sterile blood	1397:1425	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	7	2	theme	blood	1421:1425	arg1	need					1373:1376	the need	1369:1376	the need for complex media	1369:1394	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	2	3	theme	glucose	537:543	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	3	theme	glucose	537:543	arg1	1.0 g/L					545:551	glucose 1.0 g/L	537:551	glucose 1.0 g/L	537:551	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	7	4	theme	sterile	1413:1419	arg1	blood					1421:1425	sterile blood	1413:1425	sterile blood	1413:1425	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	2	5	theme	agar	471:474	arg1	2.5 g/L					491:497	agar [Yeast extract 2.5 g/L	471:497	agar [Yeast extract 2.5 g/L	471:497	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	6	dep	Count	459:463	arg1	2.5 g/L					491:497	agar [Yeast extract 2.5 g/L	471:497	agar [Yeast extract 2.5 g/L	471:497	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	7	theme	effective	616:624	arg1	medium					632:637	an effective solid medium	613:637	an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms	613:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	7	theme	effective	616:624	arg1	formulation					431:441	the commercial agar formulation	411:441	the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C]	411:590	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	1	8	theme	non-tuberculous	163:177	arg1	NTMs					193:196	NTMs	193:196	NTMs	193:196	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	8	theme	non-tuberculous	163:177	arg1	mycobacteria					179:190	non-tuberculous mycobacteria	163:190	non-tuberculous mycobacteria (NTMs)	163:197	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	9	theme	Egg	268:270	arg1	medium					272:277	Egg medium	268:277	Ogawa Egg medium	262:277	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	3	10	theme	medium-	852:858	arg1	maintenance/storage					872:890	the medium- to longterm maintenance/storage	848:890	the medium- to longterm maintenance/storage of these organisms	848:909	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	5	11	theme	NTM	1117:1119	arg1	culture					1102:1108	culture	1102:1108	culture of the NTM	1102:1119	Following this, slopes were broken out and culture of the NTM attempted.					
34333047	4	12	dep	organisms	933:941	arg1	abscessus					915:923	M. abscessus complex organisms (n = 33),	912:951	abscessus	915:923	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
34333047	1	13	theme	composition	352:362	arg1	complexity					364:373	composition complexity	352:373	composition complexity	352:373	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	0	14	theme	Mycobacterium	97:109	arg1	organisms					129:137	Mycobacterium abscessus complex organisms	97:137	Mycobacterium abscessus complex organisms	97:137	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	1	15	theme	complexity	364:373	arg1	disadvatages					336:347	disadvatages	336:347	disadvatages of composition complexity, availability and cost	336:396	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	3	16	theme	organisms	901:909	arg1	maintenance/storage					872:890	the medium- to longterm maintenance/storage	848:890	the medium- to longterm maintenance/storage of these organisms	848:909	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	1	17	theme	mycobacteria	179:190	arg1	culture					152:158	Traditional culture	140:158	Traditional culture of non-tuberculous mycobacteria (NTMs)	140:197	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	2	18	dep	formulation	431:441	arg1	SPC					466:468	SPC	466:468	SPC	466:468	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	18	dep	formulation	431:441	arg1	digest					511:516	pancreatic digest	500:516	pancreatic digest of casein 5.0 g/L	500:534	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	18	dep	formulation	431:441	arg1	1.0 g/L					545:551	glucose 1.0 g/L	537:551	glucose 1.0 g/L	537:551	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	18	dep	formulation	431:441	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	18	dep	formulation	431:441	arg1	15.0 g/L					559:566	agar 15.0 g/L	554:566	agar 15.0 g/L	554:566	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	7	19	theme	complex	1382:1388	arg1	media					1390:1394	complex media	1382:1394	complex media	1382:1394	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	4	20	theme	complex	925:931	arg1	n = 33					944:949	n = 33	944:949	n = 33	944:949	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
34333047	4	20	theme	complex	925:931	arg1	organisms					933:941	complex organisms	925:941	complex organisms (n = 33)	925:950	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
34333047	2	21	theme	pancreatic	500:509	arg1	digest					511:516	pancreatic digest	500:516	pancreatic digest of casein 5.0 g/L	500:534	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	21	theme	pancreatic	500:509	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	0	22	theme	simple	2:7	arg1	method					39:44	A simple, efficient and cost-effective method	0:44	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.	0:138	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	1	23	theme	availability	376:387	arg1	disadvatages					336:347	disadvatages	336:347	disadvatages of composition complexity, availability and cost	336:396	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	3	24	dep	medium-	852:858	arg1	to					860:861	to	860:861	to	860:861	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	3	24	dep	medium-	852:858	arg1	longterm					863:870	longterm	863:870	longterm	863:870	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	2	25	theme	organisms	722:730	arg1	enumeration					647:657	enumeration	647:657	enumeration	647:657	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	25	theme	organisms	722:730	arg1	manipulation					674:685	laboratory manipulation	663:685	laboratory manipulation	663:685	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	7	26	theme	SPCA	1282:1285	arg1	slopes					1287:1292	SPCA slopes	1282:1292	SPCA slopes	1282:1292	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	2	27	theme	Standard	444:451	arg1	SPC					466:468	SPC	466:468	SPC	466:468	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	27	theme	Standard	444:451	arg1	digest					511:516	pancreatic digest	500:516	pancreatic digest of casein 5.0 g/L	500:534	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	27	theme	Standard	444:451	arg1	1.0 g/L					545:551	glucose 1.0 g/L	537:551	glucose 1.0 g/L	537:551	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	27	theme	Standard	444:451	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	27	theme	Standard	444:451	arg1	15.0 g/L					559:566	agar 15.0 g/L	554:566	agar 15.0 g/L	554:566	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	7	28	theme	slopes	1287:1292	arg1	employment					1268:1277	the cost-effective employment	1249:1277	the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing	1249:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	0	29	theme	efficient	10:18	arg1	method					39:44	A simple, efficient and cost-effective method	0:44	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.	0:138	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	1	30	theme	defined	283:289	arg1	media					291:295	defined media	283:295	defined media (Middlebrook formulations)	283:322	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	30	theme	defined	283:289	arg1	formulations					310:321	Middlebrook formulations	298:321	Middlebrook formulations	298:321	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	0	31	theme	complex	121:127	arg1	organisms					129:137	Mycobacterium abscessus complex organisms	97:137	Mycobacterium abscessus complex organisms	97:137	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	2	32	theme	solid	626:630	arg1	medium					632:637	an effective solid medium	613:637	an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms	613:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	32	theme	solid	626:630	arg1	formulation					431:441	the commercial agar formulation	411:441	the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C]	411:590	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	33	dep	enumeration	647:657	arg1	the					643:645	the	643:645	the	643:645	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	34	theme	agar	426:429	arg1	medium					632:637	an effective solid medium	613:637	an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms	613:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	34	theme	agar	426:429	arg1	formulation					431:441	the commercial agar formulation	411:441	the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C]	411:590	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	0	35	theme	abscessus	111:119	arg1	organisms					129:137	Mycobacterium abscessus complex organisms	97:137	Mycobacterium abscessus complex organisms	97:137	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	0	36	dep	medium-	50:56	arg1	longterm					61:68	longterm	61:68	longterm	61:68	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	0	36	dep	medium-	50:56	arg1	to					58:59	to	58:59	to	58:59	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	2	37	theme	commercial	415:424	arg1	medium					632:637	an effective solid medium	613:637	an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms	613:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	37	theme	commercial	415:424	arg1	formulation					431:441	the commercial agar formulation	411:441	the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C]	411:590	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	0	38	theme	cost-effective	24:37	arg1	method					39:44	A simple, efficient and cost-effective method	0:44	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.	0:138	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	2	39	theme	5.0 g/L	528:534	arg1	digest					511:516	pancreatic digest	500:516	pancreatic digest of casein 5.0 g/L	500:534	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	39	theme	5.0 g/L	528:534	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	1	40	theme	cost	393:396	arg1	disadvatages					336:347	disadvatages	336:347	disadvatages of composition complexity, availability and cost	336:396	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	7	41	theme	abscessus	1340:1348	arg1	organisms					1350:1358	M. abscessus organisms	1337:1358	M. abscessus organisms	1337:1358	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	1	42	theme	egg-based	212:220	arg1	formulations					222:233	egg-based formulations	212:233	egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium)	212:278	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	4	43	theme	SPCA	974:977	arg1	slopes					979:984	SPCA slopes	974:984	SPCA slopes	974:984	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
34333047	1	44	theme	Middlebrook	298:308	arg1	media					291:295	defined media	283:295	defined media (Middlebrook formulations)	283:322	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	44	theme	Middlebrook	298:308	arg1	formulations					310:321	Middlebrook formulations	298:321	Middlebrook formulations	298:321	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	0	45	theme	organisms	129:137	arg1	storage					86:92	storage	86:92	storage	86:92	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	0	45	theme	organisms	129:137	arg1	maintenance					70:80	maintenance	70:80	maintenance	70:80	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	7	46	theme	organisms	1350:1358	arg1	maintenance					1322:1332	longterm maintenance	1313:1332	longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing	1313:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	7	47	theme	cost-effective	1253:1266	arg1	employment					1268:1277	the cost-effective employment	1249:1277	the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing	1249:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	3	48	theme	commercial	764:773	arg1	availability					775:786	commercial availability	764:786	commercial availability	764:786	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	1	49	dep	Lowenstein-Jensen	236:252	arg1	medium					254:259	medium	254:259	Lowenstein-Jensen medium	236:259	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	2	50	theme	laboratory	663:672	arg1	manipulation					674:685	laboratory manipulation	663:685	laboratory manipulation	663:685	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	51	theme	casein	521:526	arg1	5.0 g/L					528:534	casein 5.0 g/L	521:534	casein 5.0 g/L	521:534	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	3	52	theme	relative	743:750	arg1	simplicity					752:761	its relative simplicity	739:761	its relative simplicity	739:761	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	2	53	theme	agar	554:557	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	53	theme	agar	554:557	arg1	15.0 g/L					559:566	agar 15.0 g/L	554:566	agar 15.0 g/L	554:566	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	7	54	theme	M.	1337:1338	arg1	organisms					1350:1358	M. abscessus organisms	1337:1358	M. abscessus organisms	1337:1358	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	7	55	theme	-80 °C	1459:1464	arg1	freezing					1466:1473	continuous -80 °C freezing	1448:1473	continuous -80 °C freezing	1448:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	7	56	theme	longterm	1313:1320	arg1	maintenance					1322:1332	longterm maintenance	1313:1332	longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing	1313:1473	We therefore advocate the cost-effective employment of SPCA slopes for the medium- to longterm maintenance of M. abscessus organisms, without the need for complex media, availability of sterile blood and requirements for continuous -80 °C freezing.					
34333047	1	57	contain	have	331:334	arg1	which					325:329	which	325:329	which	325:329	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	57	contain	have	331:334	arg2	disadvatages					336:347	disadvatages	336:347	disadvatages of composition complexity, availability and cost	336:396	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	3	58	theme	inexpensive	792:802	arg1	cost					804:807	inexpensive cost	792:807	inexpensive cost	792:807	Given its relative simplicity, commercial availability and inexpensive cost, we wished to evaluate its utility for the medium- to longterm maintenance/storage of these organisms.					
34333047	1	59	dep	Ogawa	262:266	arg1	medium					272:277	Egg medium	268:277	Ogawa Egg medium	262:277	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	60	dep	formulations	222:233	arg1	Lowenstein-Jensen					236:252	Lowenstein-Jensen	236:252	Lowenstein-Jensen	236:252	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	1	60	dep	formulations	222:233	arg1	Ogawa					262:266	Ogawa	262:266	Ogawa	262:266	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	2	61	theme	complex	714:720	arg1	organisms					722:730	Mycobacterium abscessus complex organisms	690:730	Mycobacterium abscessus complex organisms	690:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	62	theme	extract	483:489	arg1	2.5 g/L					491:497	agar [Yeast extract 2.5 g/L	471:497	agar [Yeast extract 2.5 g/L	471:497	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	4	63	theme	M.	912:913	arg1	n = 33					944:949	n = 33	944:949	n = 33	944:949	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
34333047	4	63	theme	M.	912:913	arg1	organisms					933:941	complex organisms	925:941	complex organisms (n = 33)	925:950	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
34333047	6	64	theme	culture	1158:1164	arg1	viability					1166:1174	NTM culture viability	1154:1174	NTM culture viability	1154:1174	All slopes maintained NTM culture viability and were able to initiate growth six months later.					
34333047	2	65	theme	abscessus	704:712	arg1	organisms					722:730	Mycobacterium abscessus complex organisms	690:730	Mycobacterium abscessus complex organisms	690:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	1	66	theme	Traditional	140:150	arg1	culture					152:158	Traditional culture	140:158	Traditional culture of non-tuberculous mycobacteria (NTMs)	140:197	Traditional culture of non-tuberculous mycobacteria (NTMs) has involved egg-based formulations (Lowenstein-Jensen medium, Ogawa Egg medium) or defined media (Middlebrook formulations), which have disadvatages of composition complexity, availability and cost.					
34333047	0	67	theme	medium-	50:56	arg1	maintenance					70:80	maintenance	70:80	maintenance	70:80	A simple, efficient and cost-effective method for medium- to longterm maintenance and storage of Mycobacterium abscessus complex organisms.					
34333047	6	68	theme	NTM	1154:1156	arg1	viability					1166:1174	NTM culture viability	1154:1174	NTM culture viability	1154:1174	All slopes maintained NTM culture viability and were able to initiate growth six months later.					
34333047	2	69	theme	Mycobacterium	690:702	arg1	organisms					722:730	Mycobacterium abscessus complex organisms	690:730	Mycobacterium abscessus complex organisms	690:730	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	70	theme	Plate	453:457	arg1	SPC					466:468	SPC	466:468	SPC	466:468	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	70	theme	Plate	453:457	arg1	digest					511:516	pancreatic digest	500:516	pancreatic digest of casein 5.0 g/L	500:534	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	70	theme	Plate	453:457	arg1	1.0 g/L					545:551	glucose 1.0 g/L	537:551	glucose 1.0 g/L	537:551	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	70	theme	Plate	453:457	arg1	Count					459:463	Standard Plate Count	444:463	Standard Plate Count	444:463	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	2	70	theme	Plate	453:457	arg1	15.0 g/L					559:566	agar 15.0 g/L	554:566	agar 15.0 g/L	554:566	Previously, the commercial agar formulation, Standard Plate Count (SPC) agar [Yeast extract 2.5 g/L, pancreatic digest of casein 5.0 g/L, glucose 1.0 g/L, agar 15.0 g/L, pH 7.0 ± 0.2 at 25 °C] has been shown to be an effective solid medium for the enumeration and laboratory manipulation of Mycobacterium abscessus complex organisms.					
34333047	4	71	theme	ambient	1023:1029	arg1	temperature					1031:1041	ambient temperature	1023:1041	ambient temperature	1023:1041	M. abscessus complex organisms (n = 33), were inoculated onto SPCA slopes and stored undistubed in the dark at ambient temperature for six months.					
32822104	0	0	theme	stem	114:117	arg1	cells					119:123	human induced pluripotent stem cells	88:123	human induced pluripotent stem cells	88:123	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	3	1	from	hiPSCs	476:481	arg1	Matrigel					486:493	Matrigel	486:493	Matrigel	486:493	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	3	1	from	hiPSCs	476:481	arg1	laminin					498:504	laminin	498:504	laminin	498:504	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	9	2	theme	Transient	1028:1036	arg1	knockdown					1038:1046	Transient knockdown	1028:1046	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation	1028:1112	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	0	3	theme	pluripotent	102:112	arg1	cells					119:123	human induced pluripotent stem cells	88:123	human induced pluripotent stem cells	88:123	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	6	4	from	contrast	738:745	arg1	flat					783:786	flat	783:786	flat	783:786	In contrast, on laminin 511-E8, the hiPSCs were flat and YAP was active.					
32822104	6	4	from	contrast	738:745	arg1	hiPSCs					771:776	the hiPSCs	767:776	the hiPSCs	767:776	In contrast, on laminin 511-E8, the hiPSCs were flat and YAP was active.					
32822104	11	5	theme	chondrogenic	1498:1509	arg1	differentiation					1511:1525	the chondrogenic differentiation	1494:1525	the chondrogenic differentiation of hiPSCs	1494:1535	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	9	6	from	failure	1224:1230	arg1	hiPSCs					1260:1265	hiPSCs	1260:1265	hiPSCs on laminin 511-E8	1260:1283	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	9	7	theme	YAP	1197:1199	arg1	activation					1183:1192	the activation	1179:1192	the activation of YAP	1179:1199	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	9	7	theme	YAP	1197:1199	arg1	responsible					1204:1214	responsible	1204:1214	responsible	1204:1214	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	8	8	theme	Subsequent	935:944	arg1	culture					946:952	Subsequent culture	935:952	Subsequent culture of the aggregates in chondrogenic medium	935:993	Subsequent culture of the aggregates in chondrogenic medium resulted in cartilage formation.					
32822104	2	9	theme	culture	307:313	arg1	conditions					315:324	culture conditions	307:324	culture conditions	307:324	The molecular mechanisms that translate culture conditions to the chondrogenic differentiation of hiPSCs remain to be analyzed.					
32822104	10	10	theme	laminin	1334:1340	arg1	hiPSCs					1349:1354	laminin 511-E8 hiPSCs	1334:1354	laminin 511-E8 hiPSCs	1334:1354	Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation.					
32822104	0	11	theme	cells	119:123	arg1	differentiation					69:83	chondrogenic differentiation	56:83	chondrogenic differentiation of human induced pluripotent stem cells	56:123	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	8	12	theme	cartilage	1007:1015	arg1	formation					1017:1025	cartilage formation	1007:1025	cartilage formation	1007:1025	Subsequent culture of the aggregates in chondrogenic medium resulted in cartilage formation.					
32822104	10	13	theme	cartilage	1371:1379	arg1	formation					1381:1389	partial cartilage formation	1363:1389	partial cartilage formation	1363:1389	Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation.					
32822104	9	14	theme	YAP	1051:1053	arg1	knockdown					1038:1046	Transient knockdown	1028:1046	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation	1028:1112	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	11	15	theme	differentiation	1597:1611	arg1	methods					1613:1619	clinically applicable chondrogenic differentiation methods	1562:1619	clinically applicable chondrogenic differentiation methods	1562:1619	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	10	16	theme	partial	1363:1369	arg1	formation					1381:1389	partial cartilage formation	1363:1389	partial cartilage formation	1363:1389	Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation.					
32822104	3	17	theme	medium	563:568	arg1	constant					570:577	the chondrogenic medium constant	546:577	the chondrogenic medium constant	546:577	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	1	18	theme	promising	178:186	arg1	source					193:198	a promising cell source	176:198	a promising cell source for the creation of cartilage to treat articular cartilage damage	176:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	18	theme	promising	178:186	arg1	cells					157:161	Human induced pluripotent stem cells	126:161	Human induced pluripotent stem cells (hiPSCs)	126:170	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	3	19	theme	substrates	429:438	arg1	effects					410:416	the effects	406:416	the effects of culture substrates	406:438	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	11	20	theme	applicable	1573:1582	arg1	methods					1613:1619	clinically applicable chondrogenic differentiation methods	1562:1619	clinically applicable chondrogenic differentiation methods	1562:1619	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	3	21	theme	constant	570:577	arg1	composition					531:541	the composition	527:541	the composition of the chondrogenic medium constant	527:577	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	7	22	theme	511-E8	829:834	arg1	hiPSCs					836:841	the laminin 511-E8 hiPSCs	817:841	the laminin 511-E8 hiPSCs	817:841	Treating the laminin 511-E8 hiPSCs in a bioreactor caused cell aggregates, in which the cells were round and YAP was inactive.					
32822104	1	23	theme	cell	188:191	arg1	source					193:198	a promising cell source	176:198	a promising cell source for the creation of cartilage to treat articular cartilage damage	176:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	23	theme	cell	188:191	arg1	cells					157:161	Human induced pluripotent stem cells	126:161	Human induced pluripotent stem cells (hiPSCs)	126:170	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	0	24	theme	substrate-associated	8:27	arg1	inactivation					33:44	Culture substrate-associated YAP inactivation	0:44	Culture substrate-associated YAP inactivation	0:44	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	8	25	theme	aggregates	961:970	arg1	culture					946:952	Subsequent culture	935:952	Subsequent culture of the aggregates in chondrogenic medium	935:993	Subsequent culture of the aggregates in chondrogenic medium resulted in cartilage formation.					
32822104	2	26	theme	chondrogenic	333:344	arg1	differentiation					346:360	the chondrogenic differentiation	329:360	the chondrogenic differentiation of hiPSCs	329:370	The molecular mechanisms that translate culture conditions to the chondrogenic differentiation of hiPSCs remain to be analyzed.					
32822104	0	27	theme	Culture	0:6	arg1	inactivation					33:44	Culture substrate-associated YAP inactivation	0:44	Culture substrate-associated YAP inactivation	0:44	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	11	28	theme	chondrogenic	1584:1595	arg1	methods					1613:1619	clinically applicable chondrogenic differentiation methods	1562:1619	clinically applicable chondrogenic differentiation methods	1562:1619	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	9	29	from	knockdown	1038:1046	arg1	hiPSCs					1058:1063	hiPSCs	1058:1063	hiPSCs around the start of chondrogenic differentiation	1058:1112	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	8	30	theme	chondrogenic	975:986	arg1	medium					988:993	chondrogenic medium	975:993	chondrogenic medium	975:993	Subsequent culture of the aggregates in chondrogenic medium resulted in cartilage formation.					
32822104	1	31	theme	Human	126:130	arg1	cells					157:161	Human induced pluripotent stem cells	126:161	Human induced pluripotent stem cells (hiPSCs)	126:170	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	31	theme	Human	126:130	arg1	source					193:198	a promising cell source	176:198	a promising cell source for the creation of cartilage to treat articular cartilage damage	176:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	31	theme	Human	126:130	arg1	hiPSCs					164:169	hiPSCs	164:169	hiPSCs	164:169	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	7	32	theme	laminin	821:827	arg1	hiPSCs					836:841	the laminin 511-E8 hiPSCs	817:841	the laminin 511-E8 hiPSCs	817:841	Treating the laminin 511-E8 hiPSCs in a bioreactor caused cell aggregates, in which the cells were round and YAP was inactive.					
32822104	0	33	theme	YAP	29:31	arg1	inactivation					33:44	Culture substrate-associated YAP inactivation	0:44	Culture substrate-associated YAP inactivation	0:44	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	11	34	theme	hiPSCs	1530:1535	arg1	differentiation					1511:1525	the chondrogenic differentiation	1494:1525	the chondrogenic differentiation of hiPSCs	1494:1535	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	10	35	theme	YAP	1316:1318	arg1	inhibitors					1320:1329	YAP inhibitors	1316:1329	YAP inhibitors	1316:1329	Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation.					
32822104	1	36	theme	induced	132:138	arg1	cells					157:161	Human induced pluripotent stem cells	126:161	Human induced pluripotent stem cells (hiPSCs)	126:170	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	36	theme	induced	132:138	arg1	source					193:198	a promising cell source	176:198	a promising cell source for the creation of cartilage to treat articular cartilage damage	176:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	36	theme	induced	132:138	arg1	hiPSCs					164:169	hiPSCs	164:169	hiPSCs	164:169	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	0	37	theme	chondrogenic	56:67	arg1	differentiation					69:83	chondrogenic differentiation	56:83	chondrogenic differentiation of human induced pluripotent stem cells	56:123	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	2	38	theme	hiPSCs	365:370	arg1	differentiation					346:360	the chondrogenic differentiation	329:360	the chondrogenic differentiation of hiPSCs	329:370	The molecular mechanisms that translate culture conditions to the chondrogenic differentiation of hiPSCs remain to be analyzed.					
32822104	9	39	theme	chondrogenic	1085:1096	arg1	differentiation					1098:1112	chondrogenic differentiation	1085:1112	chondrogenic differentiation	1085:1112	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	6	40	from	flat	783:786	arg1	contrast					738:745	contrast	738:745	contrast	738:745	In contrast, on laminin 511-E8, the hiPSCs were flat and YAP was active.					
32822104	9	41	theme	differentiation	1098:1112	arg1	start					1076:1080	the start	1072:1080	the start of chondrogenic differentiation	1072:1112	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	1	42	theme	cartilage	220:228	arg1	creation					208:215	the creation	204:215	the creation of cartilage to treat articular cartilage damage	204:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	5	43	from	round	682:686	arg1	Matrigel					656:663	Matrigel	656:663	Matrigel	656:663	On Matrigel, the hiPSCs were round and yes-associated protein (YAP) was inactive.					
32822104	9	44	from	hiPSCs	1260:1265	arg1	failure					1224:1230	the failure	1220:1230	the failure of cartilage formation from hiPSCs on laminin 511-E8	1220:1283	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	9	44	from	hiPSCs	1260:1265	arg1	formation					1245:1253	cartilage formation	1235:1253	cartilage formation from hiPSCs on laminin 511-E8	1235:1283	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	9	44	from	hiPSCs	1260:1265	arg1	laminin					1270:1276	laminin 511-E8	1270:1283	laminin 511-E8	1270:1283	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	11	45	from	effects	1461:1467	arg1	differentiation					1511:1525	the chondrogenic differentiation	1494:1525	the chondrogenic differentiation of hiPSCs	1494:1535	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	11	45	from	effects	1461:1467	arg1	developing					1551:1560	developing	1551:1560	developing clinically applicable chondrogenic differentiation methods	1551:1619	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	10	46	theme	inhibitors	1320:1329	arg1	addition					1304:1311	the addition	1300:1311	the addition of YAP inhibitors to laminin 511-E8 hiPSCs	1300:1354	Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation.					
32822104	1	47	theme	pluripotent	140:150	arg1	cells					157:161	Human induced pluripotent stem cells	126:161	Human induced pluripotent stem cells (hiPSCs)	126:170	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	47	theme	pluripotent	140:150	arg1	source					193:198	a promising cell source	176:198	a promising cell source for the creation of cartilage to treat articular cartilage damage	176:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	47	theme	pluripotent	140:150	arg1	hiPSCs					164:169	hiPSCs	164:169	hiPSCs	164:169	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	3	48	theme	culture	421:427	arg1	substrates					429:438	culture substrates	421:438	culture substrates	421:438	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	5	49	theme	yes-associated	692:705	arg1	protein					707:713	yes-associated protein	692:713	yes-associated protein (YAP)	692:719	On Matrigel, the hiPSCs were round and yes-associated protein (YAP) was inactive.					
32822104	5	49	theme	yes-associated	692:705	arg1	YAP					716:718	YAP	716:718	YAP	716:718	On Matrigel, the hiPSCs were round and yes-associated protein (YAP) was inactive.					
32822104	9	50	theme	cartilage	1235:1243	arg1	formation					1245:1253	cartilage formation	1235:1253	cartilage formation from hiPSCs on laminin 511-E8	1235:1283	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	6	51	theme	laminin	751:757	arg1	511-E8					759:764	laminin 511-E8	751:764	laminin 511-E8	751:764	In contrast, on laminin 511-E8, the hiPSCs were flat and YAP was active.					
32822104	1	52	theme	stem	152:155	arg1	cells					157:161	Human induced pluripotent stem cells	126:161	Human induced pluripotent stem cells (hiPSCs)	126:170	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	52	theme	stem	152:155	arg1	source					193:198	a promising cell source	176:198	a promising cell source for the creation of cartilage to treat articular cartilage damage	176:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	1	52	theme	stem	152:155	arg1	hiPSCs					164:169	hiPSCs	164:169	hiPSCs	164:169	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	9	53	theme	formation	1245:1253	arg1	failure					1224:1230	the failure	1220:1230	the failure of cartilage formation from hiPSCs on laminin 511-E8	1220:1283	Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8.					
32822104	2	54	theme	molecular	271:279	arg1	mechanisms					281:290	The molecular mechanisms	267:290	The molecular mechanisms that translate culture conditions to the chondrogenic differentiation of hiPSCs	267:370	The molecular mechanisms that translate culture conditions to the chondrogenic differentiation of hiPSCs remain to be analyzed.					
32822104	7	55	from	round	907:911	arg1	aggregates					871:880	cell aggregates	866:880	cell aggregates	866:880	Treating the laminin 511-E8 hiPSCs in a bioreactor caused cell aggregates, in which the cells were round and YAP was inactive.					
32822104	3	56	dep	Matrigel	486:493	arg1	511-E8					506:511	511-E8	506:511	511-E8	506:511	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	11	57	theme	culture	1472:1478	arg1	substrates					1480:1489	culture substrates	1472:1489	culture substrates	1472:1489	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	8	58	from	culture	946:952	arg1	medium					988:993	chondrogenic medium	975:993	chondrogenic medium	975:993	Subsequent culture of the aggregates in chondrogenic medium resulted in cartilage formation.					
32822104	0	59	theme	induced	94:100	arg1	cells					119:123	human induced pluripotent stem cells	88:123	human induced pluripotent stem cells	88:123	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	11	60	theme	substrates	1480:1489	arg1	effects					1461:1467	the effects	1457:1467	the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods	1457:1619	This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.					
32822104	1	61	theme	articular	239:247	arg1	damage					259:264	articular cartilage damage	239:264	articular cartilage damage	239:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
32822104	0	62	theme	human	88:92	arg1	cells					119:123	human induced pluripotent stem cells	88:123	human induced pluripotent stem cells	88:123	Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.					
32822104	10	63	theme	511-E8	1342:1347	arg1	hiPSCs					1349:1354	laminin 511-E8 hiPSCs	1334:1354	laminin 511-E8 hiPSCs	1334:1354	Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation.					
32822104	3	64	theme	chondrogenic	550:561	arg1	constant					570:577	the chondrogenic medium constant	546:577	the chondrogenic medium constant	546:577	To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant.					
32822104	7	65	theme	cell	866:869	arg1	aggregates					871:880	cell aggregates	866:880	cell aggregates	866:880	Treating the laminin 511-E8 hiPSCs in a bioreactor caused cell aggregates, in which the cells were round and YAP was inactive.					
32822104	1	66	theme	cartilage	249:257	arg1	damage					259:264	articular cartilage damage	239:264	articular cartilage damage	239:264	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage.					
31971484	10	0	theme	bone	1567:1570	arg1	engineering					1579:1589	bone tissue engineering	1567:1589	bone tissue engineering	1567:1589	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	4	1	theme	XRD	538:540	arg1	results					542:548	The FTIR and XRD results	525:548	results	542:548	The FTIR and XRD results indicate that PVA, SA and HA could be uniformly compounded.					
31971484	5	2	theme	samples	674:680	arg1	porosity					658:665	porosity	658:665	porosity	658:665	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	5	2	theme	samples	674:680	arg1	properties					625:634	The mechanical properties	610:634	The mechanical properties	610:634	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	5	2	theme	samples	674:680	arg1	content					646:652	moisture content	637:652	moisture content	637:652	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	1	3	theme	polyvinyl	137:145	arg1	hydrogels					208:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels	130:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	130:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	6	4	theme	weight	919:924	arg1	ratio					926:930	the weight ratio	915:930	the weight ratio of PVA/SA/HA	915:943	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	9	5	theme	phosphatase	1307:1317	arg1	expressions					1334:1344	Alkaline phosphatase (ALP) activity expressions	1298:1344	Alkaline phosphatase (ALP) activity expressions	1298:1344	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	10	6	theme	PVA/SA/HA	1496:1504	arg1	candidate					1537:1545	a promising candidate	1525:1545	a promising candidate for bone repair and bone tissue engineering	1525:1589	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	10	6	theme	PVA/SA/HA	1496:1504	arg1	hydrogels					1506:1514	the prepared PVA/SA/HA hydrogels	1483:1514	the prepared PVA/SA/HA hydrogels	1483:1514	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	1	7	theme	alcohol/sodium	147:160	arg1	hydrogels					208:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels	130:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	130:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	8	8	from	effects	1247:1253	arg1	cell					1267:1270	MC3T3-E1 cell growth and proliferation	1258:1295	MC3T3-E1 cell growth and proliferation	1258:1295	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	5	9	theme	moisture	637:644	arg1	content					646:652	moisture content	637:652	moisture content	637:652	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	9	10	theme	cells	1459:1463	arg1	activity					1443:1450	the ALP activity	1435:1450	the ALP activity of the cells	1435:1463	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	8	11	theme	culture	1176:1182	arg1	results					1184:1190	cell culture results	1171:1190	cell culture results	1171:1190	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	7	12	theme	sheet-like	1100:1109	arg1	nanocrystals					1114:1125	sheet-like HA nanocrystals	1100:1125	sheet-like HA nanocrystals	1100:1125	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	7	13	theme	In	958:959	arg1	biodegradation					967:980	In vitro biodegradation	958:980	In vitro biodegradation	958:980	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	1	14	theme	PVA/SA/HA	187:195	arg1	hydrogels					208:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels	130:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	130:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	1	15	theme	alginate/hydroxyapatite	162:184	arg1	hydrogels					208:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels	130:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	130:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	8	16	theme	cell	1171:1174	arg1	results					1184:1190	cell culture results	1171:1190	cell culture results	1171:1190	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	9	17	theme	activity	1325:1332	arg1	expressions					1334:1344	Alkaline phosphatase (ALP) activity expressions	1298:1344	Alkaline phosphatase (ALP) activity expressions	1298:1344	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	7	18	theme	HA	1111:1112	arg1	nanocrystals					1114:1125	sheet-like HA nanocrystals	1100:1125	sheet-like HA nanocrystals	1100:1125	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	8	19	dep	cell	1267:1270	arg1	growth					1272:1277	growth	1272:1277	growth	1272:1277	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	8	19	dep	cell	1267:1270	arg1	proliferation					1283:1295	proliferation	1283:1295	proliferation	1283:1295	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	3	20	theme	uniform	481:487	arg1	structure					514:522	uniform, interpenetrating porous structure	481:522	uniform, interpenetrating porous structure	481:522	The PVA/SA/HA hydrogels present uniform, interpenetrating porous structure.					
31971484	4	21	theme	FTIR	529:532	arg1	results					542:548	The FTIR and XRD results	525:548	results	542:548	The FTIR and XRD results indicate that PVA, SA and HA could be uniformly compounded.					
31971484	10	22	theme	prepared	1487:1494	arg1	candidate					1537:1545	a promising candidate	1525:1545	a promising candidate for bone repair and bone tissue engineering	1525:1589	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	10	22	theme	prepared	1487:1494	arg1	hydrogels					1506:1514	the prepared PVA/SA/HA hydrogels	1483:1514	the prepared PVA/SA/HA hydrogels	1483:1514	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	10	23	theme	bone	1551:1554	arg1	repair					1556:1561	bone repair	1551:1561	bone repair	1551:1561	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	3	24	theme	PVA/SA/HA	453:461	arg1	hydrogels					463:471	The PVA/SA/HA hydrogels	449:471	The PVA/SA/HA hydrogels	449:471	The PVA/SA/HA hydrogels present uniform, interpenetrating porous structure.					
31971484	0	25	theme	Porous	0:5	arg1	PVA/SA/HA					7:15	Porous PVA/SA/HA	0:15	Porous PVA/SA/HA	0:15	Porous PVA/SA/HA hydrogels fabricated by dual-crosslinking method for bone tissue engineering.					
31971484	1	26	theme	dual-crosslinking	291:307	arg1	method					309:314	dual-crosslinking method	291:314	dual-crosslinking method	291:314	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	9	27	theme	composite	1397:1405	arg1	hydrogels					1407:1415	composite hydrogels	1397:1415	composite hydrogels	1397:1415	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	2	28	theme	moisture	335:342	arg1	morphologies					321:332	The morphologies	317:332	The morphologies	317:332	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	2	28	theme	moisture	335:342	arg1	content					344:350	moisture content	335:350	moisture content	335:350	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	2	29	theme	hydrogels	405:413	arg1	properties					377:386	mechanical properties	366:386	mechanical properties	366:386	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	2	29	theme	hydrogels	405:413	arg1	porosity					353:360	porosity	353:360	porosity	353:360	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	2	29	theme	hydrogels	405:413	arg1	morphologies					321:332	The morphologies	317:332	The morphologies	317:332	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	2	29	theme	hydrogels	405:413	arg1	content					344:350	moisture content	335:350	moisture content	335:350	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	10	30	theme	promising	1527:1535	arg1	candidate					1537:1545	a promising candidate	1525:1545	a promising candidate for bone repair and bone tissue engineering	1525:1589	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	10	30	theme	promising	1527:1535	arg1	hydrogels					1506:1514	the prepared PVA/SA/HA hydrogels	1483:1514	the prepared PVA/SA/HA hydrogels	1483:1514	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	5	31	theme	mechanical	614:623	arg1	properties					625:634	The mechanical properties	610:634	The mechanical properties	610:634	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	1	32	theme	present	102:108	arg1	work					110:113	the present work	98:113	the present work	98:113	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	1	33	theme	composite	198:206	arg1	hydrogels					208:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels	130:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	130:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	6	34	theme	compression	757:767	arg1	41.74 ± 7.86 kPa					778:793	41.74 ± 7.86 kPa	778:793	41.74 ± 7.86 kPa	778:793	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	34	theme	compression	757:767	arg1	modulus					769:775	The optimized compression modulus	743:775	The optimized compression modulus (41.74 ± 7.86 kPa)	743:794	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	7	35	theme	PBS	1083:1085	arg1	solution					1087:1094	PBS solution	1083:1094	PBS solution	1083:1094	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	6	36	theme	hydrogels	876:884	arg1	%					856:856	79.98 ± 1.61%	844:856	79.98 ± 1.61%	844:856	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	36	theme	hydrogels	876:884	arg1	41.74 ± 7.86 kPa					778:793	41.74 ± 7.86 kPa	778:793	41.74 ± 7.86 kPa	778:793	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	36	theme	hydrogels	876:884	arg1	%					827:827	86.99 ± 0.72%	815:827	86.99 ± 0.72%	815:827	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	36	theme	hydrogels	876:884	arg1	porosity					834:841	porosity	834:841	porosity (79.98 ± 1.61%) of the composite hydrogels	834:884	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	36	theme	hydrogels	876:884	arg1	modulus					769:775	The optimized compression modulus	743:775	The optimized compression modulus (41.74 ± 7.86 kPa)	743:794	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	36	theme	hydrogels	876:884	arg1	content					806:812	moisture content	797:812	moisture content (86.99 ± 0.72%)	797:828	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	1	37	theme	hydrogels	208:216	arg1	kind					122:125	a new kind	116:125	a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	116:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	9	38	theme	Alkaline	1298:1305	arg1	phosphatase					1307:1317	Alkaline phosphatase	1298:1317	Alkaline phosphatase (ALP) activity expressions	1298:1344	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	9	38	theme	Alkaline	1298:1305	arg1	ALP					1320:1322	ALP	1320:1322	ALP	1320:1322	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	9	39	theme	nano-HA	1367:1373	arg1	crystals					1375:1382	the nano-HA crystals	1363:1382	the nano-HA crystals incorporated composite hydrogels	1363:1415	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
31971484	5	40	theme	PVA/SA/HA	732:740	arg1	ratio					723:727	the mass ratio	714:727	the mass ratio of PVA/SA/HA	714:740	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	6	41	theme	composite	866:874	arg1	hydrogels					876:884	the composite hydrogels	862:884	the composite hydrogels	862:884	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	8	42	contain	have	1230:1233	arg1	hydrogels					1220:1228	the PVA/SA/HA hydrogels	1206:1228	the PVA/SA/HA hydrogels	1206:1228	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	8	42	contain	have	1230:1233	arg2	effects					1247:1253	no negative effects	1235:1253	no negative effects on MC3T3-E1 cell growth and proliferation	1235:1295	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	8	43	theme	PVA/SA/HA	1210:1218	arg1	hydrogels					1220:1228	the PVA/SA/HA hydrogels	1206:1228	the PVA/SA/HA hydrogels	1206:1228	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	1	44	with	hydrogels	208:216	arg1	structure					231:239	tunable structure	223:239	tunable structure	223:239	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	1	44	with	hydrogels	208:216	arg1	properties					256:265	mechanical properties	245:265	mechanical properties	245:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	1	45	theme	tunable	223:229	arg1	structure					231:239	tunable structure	223:239	tunable structure	223:239	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	10	46	theme	tissue	1572:1577	arg1	engineering					1579:1589	bone tissue engineering	1567:1589	bone tissue engineering	1567:1589	It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.					
31971484	2	47	theme	mechanical	366:375	arg1	morphologies					321:332	The morphologies	317:332	The morphologies	317:332	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	2	47	theme	mechanical	366:375	arg1	properties					377:386	mechanical properties	366:386	mechanical properties	366:386	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	6	48	theme	PVA/SA/HA	935:943	arg1	ratio					926:930	the weight ratio	915:930	the weight ratio of PVA/SA/HA	915:943	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	8	49	theme	MC3T3-E1	1258:1265	arg1	cell					1267:1270	MC3T3-E1 cell growth and proliferation	1258:1295	MC3T3-E1 cell growth and proliferation	1258:1295	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	3	50	dep	uniform	481:487	arg1	interpenetrating					490:505	interpenetrating	490:505	interpenetrating	490:505	The PVA/SA/HA hydrogels present uniform, interpenetrating porous structure.					
31971484	7	51	theme	composite	1008:1016	arg1	hydrogels					1018:1026	the composite hydrogels	1004:1026	the composite hydrogels	1004:1026	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	6	52	theme	moisture	797:804	arg1	content					806:812	moisture content	797:812	moisture content (86.99 ± 0.72%)	797:828	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	52	theme	moisture	797:804	arg1	%					827:827	86.99 ± 0.72%	815:827	86.99 ± 0.72%	815:827	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	7	53	theme	hydrogels	1018:1026	arg1	mineralization					986:999	mineralization	986:999	mineralization of the composite hydrogels	986:1026	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	7	53	theme	hydrogels	1018:1026	arg1	biodegradation					967:980	In vitro biodegradation	958:980	In vitro biodegradation	958:980	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	1	54	theme	new	118:120	arg1	kind					122:125	a new kind	116:125	a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	116:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	7	55	dep	In	958:959	arg1	vitro					961:965	vitro	961:965	vitro	961:965	In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface.					
31971484	5	56	theme	mass	718:721	arg1	ratio					723:727	the mass ratio	714:727	the mass ratio of PVA/SA/HA	714:740	The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA.					
31971484	0	57	dep	hydrogels	17:25	arg1	fabricated					27:36	fabricated	27:36	hydrogels fabricated by dual-crosslinking method for bone tissue engineering	17:92	Porous PVA/SA/HA hydrogels fabricated by dual-crosslinking method for bone tissue engineering.					
31971484	0	58	theme	tissue	75:80	arg1	engineering					82:92	bone tissue engineering	70:92	bone tissue engineering	70:92	Porous PVA/SA/HA hydrogels fabricated by dual-crosslinking method for bone tissue engineering.					
31971484	3	59	theme	porous	507:512	arg1	structure					514:522	uniform, interpenetrating porous structure	481:522	uniform, interpenetrating porous structure	481:522	The PVA/SA/HA hydrogels present uniform, interpenetrating porous structure.					
31971484	2	60	theme	composite	395:403	arg1	hydrogels					405:413	the composite hydrogels	391:413	the composite hydrogels	391:413	The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail.					
31971484	8	61	theme	negative	1238:1245	arg1	effects					1247:1253	no negative effects	1235:1253	no negative effects on MC3T3-E1 cell growth and proliferation	1235:1295	Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation.					
31971484	1	62	theme	mechanical	245:254	arg1	properties					256:265	mechanical properties	245:265	mechanical properties	245:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	0	63	theme	bone	70:73	arg1	engineering					82:92	bone tissue engineering	70:92	bone tissue engineering	70:92	Porous PVA/SA/HA hydrogels fabricated by dual-crosslinking method for bone tissue engineering.					
31971484	6	64	theme	optimized	747:755	arg1	41.74 ± 7.86 kPa					778:793	41.74 ± 7.86 kPa	778:793	41.74 ± 7.86 kPa	778:793	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	6	64	theme	optimized	747:755	arg1	modulus					769:775	The optimized compression modulus	743:775	The optimized compression modulus (41.74 ± 7.86 kPa)	743:794	The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40.					
31971484	1	65	theme	porous	130:135	arg1	hydrogels					208:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels	130:216	porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties	130:265	In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method.					
31971484	9	66	theme	ALP	1439:1441	arg1	activity					1443:1450	the ALP activity	1435:1450	the ALP activity of the cells	1435:1463	Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells.					
32275718	9	0	theme	acid	1272:1275	arg1	bacteria					1277:1284	the lactic acid bacteria	1261:1284	the lactic acid bacteria community within the gut microbiota	1261:1320	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	7	1	theme	gut	959:961	arg1	weights					963:969	higher gut weights	952:969	higher gut weights	952:969	Bees fed with melezitose showed increased food uptake, higher gut weights and elevated mortality compared to bees fed a control diet.					
32275718	4	2	theme	molecular	511:519	arg1	weight					521:526	higher molecular weight	504:526	higher molecular weight	504:526	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	9	3	theme	melezitose	1210:1219	arg1	diet					1221:1224	the melezitose diet	1206:1224	the melezitose diet	1206:1224	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	11	4	theme	accumulated	1527:1537	arg1	melezitose					1539:1548	the accumulated melezitose	1523:1548	the accumulated melezitose	1523:1548	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	12	5	theme	possible	1827:1834	arg1	mechanism					1836:1844	a possible mechanism	1825:1844	a possible mechanism of pathogenesis	1825:1860	These findings confirm the causal relation between the trisaccharide melezitose and the honeydew flow disease and indicate a possible mechanism of pathogenesis.					
32275718	4	6	theme	higher	504:509	arg1	weight					521:526	higher molecular weight	504:526	higher molecular weight	504:526	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	6	7	theme	feeding	824:830	arg1	experiments					832:842	three independent feeding experiments	806:842	three independent feeding experiments with caged bees	806:858	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	12	8	theme	pathogenesis	1849:1860	arg1	mechanism					1836:1844	a possible mechanism	1825:1844	a possible mechanism of pathogenesis	1825:1860	These findings confirm the causal relation between the trisaccharide melezitose and the honeydew flow disease and indicate a possible mechanism of pathogenesis.					
32275718	12	9	theme	trisaccharide	1757:1769	arg1	melezitose					1771:1780	the trisaccharide melezitose	1753:1780	the trisaccharide melezitose	1753:1780	These findings confirm the causal relation between the trisaccharide melezitose and the honeydew flow disease and indicate a possible mechanism of pathogenesis.					
32275718	2	10	theme	monocultural	235:246	arg1	landscapes					248:257	monocultural landscapes	235:257	monocultural landscapes	235:257	In the absence of nectar, during certain times of the year or in monocultural landscapes, honey bees forage on honeydew.					
32275718	11	11	theme	capabilities	1659:1670	arg1	result					1622:1627	result	1622:1627	result of poor melezitose metabolism capabilities in the intestinal microbiota	1622:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	11	12	theme	intestinal	1567:1576	arg1	symptoms					1578:1585	severe intestinal symptoms	1560:1585	severe intestinal symptoms	1560:1585	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	6	13	theme	independent	812:822	arg1	experiments					832:842	three independent feeding experiments	806:842	three independent feeding experiments with caged bees	806:858	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	4	14	with	sugars	492:497	arg1	weight					521:526	higher molecular weight	504:526	higher molecular weight	504:526	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	9	15	theme	species	1238:1244	arg1	composition					1246:1256	the species composition	1234:1256	the species composition of the lactic acid bacteria community within the gut microbiota	1234:1320	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	11	16	from	microbiota	1690:1699	arg1	result					1622:1627	result	1622:1627	result of poor melezitose metabolism capabilities in the intestinal microbiota	1622:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	1	17	theme	collected	152:160	arg1	nectar					162:167	collected nectar	152:167	collected nectar	152:167	In general, honey bees (Apis mellifera L.) feed on honey produced from collected nectar.					
32275718	8	18	theme	melezitose-fed	1146:1159	arg1	bees					1161:1164	melezitose-fed bees	1146:1164	melezitose-fed bees	1146:1164	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	19	theme	swollen	1074:1080	arg1	abdomen					1082:1088	swollen abdomen	1074:1088	swollen abdomen	1074:1088	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	11	20	theme	severe	1560:1565	arg1	symptoms					1578:1585	severe intestinal symptoms	1560:1585	severe intestinal symptoms	1560:1585	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	7	21	theme	control	1017:1023	arg1	diet					1025:1028	a control diet	1015:1028	a control diet	1015:1028	Bees fed with melezitose showed increased food uptake, higher gut weights and elevated mortality compared to bees fed a control diet.					
32275718	2	22	theme	year	224:227	arg1	times					211:215	certain times	203:215	certain times of the year	203:227	In the absence of nectar, during certain times of the year or in monocultural landscapes, honey bees forage on honeydew.					
32275718	11	23	theme	intestinal	1679:1688	arg1	microbiota					1690:1699	the intestinal microbiota	1675:1699	the intestinal microbiota	1675:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	3	24	theme	plant	386:390	arg1	species					392:398	plant species	386:398	plant species	386:398	Honeydew is excreted by different herbivores of the order Hemiptera that consume phloem sap of plant species.					
32275718	0	25	theme	trisaccharide	4:16	arg1	melezitose					18:27	The trisaccharide melezitose	0:27	The trisaccharide melezitose	0:27	The trisaccharide melezitose impacts honey bees and their intestinal microbiota.					
32275718	4	26	theme	sugars	492:497	arg1	sugars					470:475	sugars	470:475	sugars	470:475	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	26	theme	sugars	492:497	arg1	sugars					492:497	additional sugars	481:497	additional sugars	481:497	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	26	theme	sugars	492:497	arg1	variety					459:465	a higher variety	450:465	a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew	450:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	8	27	theme	disease	1048:1054	arg1	symptoms					1056:1063	severe disease symptoms	1041:1063	severe disease symptoms	1041:1063	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	27	theme	disease	1048:1054	arg1	movement					1120:1127	impaired movement	1111:1127	impaired movement	1111:1127	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	27	theme	disease	1048:1054	arg1	tipping					1099:1105	abdomen tipping	1091:1105	abdomen tipping	1091:1105	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	27	theme	disease	1048:1054	arg1	abdomen					1082:1088	swollen abdomen	1074:1088	swollen abdomen	1074:1088	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	4	28	with	sugars	470:475	arg1	weight					521:526	higher molecular weight	504:526	higher molecular weight	504:526	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	3	29	theme	phloem	372:377	arg1	sap					379:381	phloem sap	372:381	phloem sap of plant species	372:398	Honeydew is excreted by different herbivores of the order Hemiptera that consume phloem sap of plant species.					
32275718	12	30	theme	flow	1799:1802	arg1	disease					1804:1810	the honeydew flow disease	1786:1810	the honeydew flow disease	1786:1810	These findings confirm the causal relation between the trisaccharide melezitose and the honeydew flow disease and indicate a possible mechanism of pathogenesis.					
32275718	4	31	theme	additional	481:490	arg1	sugars					492:497	additional sugars	481:497	additional sugars	481:497	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	2	32	theme	honey	260:264	arg1	bees					266:269	honey bees	260:269	honey bees	260:269	In the absence of nectar, during certain times of the year or in monocultural landscapes, honey bees forage on honeydew.					
32275718	11	33	theme	bees	1604:1607	arg1	death					1591:1595	death	1591:1595	death of the bees	1591:1607	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	11	33	theme	bees	1604:1607	arg1	symptoms					1578:1585	severe intestinal symptoms	1560:1585	severe intestinal symptoms	1560:1585	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	3	34	theme	different	315:323	arg1	herbivores					325:334	different herbivores	315:334	different herbivores of the order Hemiptera that consume phloem sap of plant species	315:398	Honeydew is excreted by different herbivores of the order Hemiptera that consume phloem sap of plant species.					
32275718	9	35	theme	lactic	1265:1270	arg1	bacteria					1277:1284	the lactic acid bacteria	1261:1284	the lactic acid bacteria community within the gut microbiota	1261:1320	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	11	36	from	capabilities	1659:1670	arg1	microbiota					1690:1699	the intestinal microbiota	1675:1699	the intestinal microbiota	1675:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	1	37	theme	honey	93:97	arg1	mellifera					110:118	Apis mellifera L.	105:121	Apis mellifera L.	105:121	In general, honey bees (Apis mellifera L.) feed on honey produced from collected nectar.					
32275718	1	37	theme	honey	93:97	arg1	bees					99:102	honey bees	93:102	honey bees (Apis mellifera L.)	93:122	In general, honey bees (Apis mellifera L.) feed on honey produced from collected nectar.					
32275718	12	38	theme	honeydew	1790:1797	arg1	disease					1804:1810	the honeydew flow disease	1786:1810	the honeydew flow disease	1786:1810	These findings confirm the causal relation between the trisaccharide melezitose and the honeydew flow disease and indicate a possible mechanism of pathogenesis.					
32275718	4	39	dep	nectar	418:423	arg1	comparison					404:413	comparison	404:413	comparison	404:413	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	11	40	from	result	1622:1627	arg1	microbiota					1690:1699	the intestinal microbiota	1675:1699	the intestinal microbiota	1675:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	4	41	theme	honeydew	598:605	arg1	constituent					583:593	a major constituent	575:593	a major constituent of honeydew	575:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	41	theme	honeydew	598:605	arg1	melezitose					552:561	the trisaccharide melezitose	534:561	the trisaccharide melezitose that can be a major constituent of honeydew	534:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	7	42	theme	elevated	975:982	arg1	mortality					984:992	elevated mortality	975:992	elevated mortality	975:992	Bees fed with melezitose showed increased food uptake, higher gut weights and elevated mortality compared to bees fed a control diet.					
32275718	6	43	theme	flow	791:794	arg1	disease					796:802	the so called 'honeydew flow disease'	767:803	the so called 'honeydew flow disease'	767:803	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	6	44	theme	consecutive	878:888	arg1	years					890:894	consecutive years	878:894	consecutive years	878:894	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	3	45	theme	species	392:398	arg1	sap					379:381	phloem sap	372:381	phloem sap of plant species	372:398	Honeydew is excreted by different herbivores of the order Hemiptera that consume phloem sap of plant species.					
32275718	0	46	theme	honey	37:41	arg1	bees					43:46	honey bees	37:46	honey bees	37:46	The trisaccharide melezitose impacts honey bees and their intestinal microbiota.					
32275718	9	47	theme	community	1286:1294	arg1	bacteria					1277:1284	the lactic acid bacteria	1261:1284	the lactic acid bacteria community within the gut microbiota	1261:1320	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	8	48	theme	impaired	1111:1118	arg1	movement					1120:1127	impaired movement	1111:1127	impaired movement	1111:1127	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	6	49	theme	honeydew	782:789	arg1	disease					796:802	the so called 'honeydew flow disease'	767:803	the so called 'honeydew flow disease'	767:803	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	12	50	theme	causal	1729:1734	arg1	relation					1736:1743	the causal relation	1725:1743	the causal relation between the trisaccharide melezitose and the honeydew flow disease	1725:1810	These findings confirm the causal relation between the trisaccharide melezitose and the honeydew flow disease and indicate a possible mechanism of pathogenesis.					
32275718	9	51	theme	gut	1307:1309	arg1	microbiota					1311:1320	the gut microbiota	1303:1320	the gut microbiota	1303:1320	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	3	52	theme	order	343:347	arg1	Hemiptera					349:357	the order Hemiptera	339:357	the order Hemiptera that consume phloem sap of plant species	339:398	Honeydew is excreted by different herbivores of the order Hemiptera that consume phloem sap of plant species.					
32275718	4	53	theme	higher	452:457	arg1	sugars					470:475	sugars	470:475	sugars	470:475	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	53	theme	higher	452:457	arg1	sugars					492:497	additional sugars	481:497	additional sugars	481:497	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	53	theme	higher	452:457	arg1	variety					459:465	a higher variety	450:465	a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew	450:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	8	54	theme	severe	1041:1046	arg1	symptoms					1056:1063	severe disease symptoms	1041:1063	severe disease symptoms	1041:1063	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	54	theme	severe	1041:1046	arg1	movement					1120:1127	impaired movement	1111:1127	impaired movement	1111:1127	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	54	theme	severe	1041:1046	arg1	tipping					1099:1105	abdomen tipping	1091:1105	abdomen tipping	1091:1105	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	54	theme	severe	1041:1046	arg1	abdomen					1082:1088	swollen abdomen	1074:1088	swollen abdomen	1074:1088	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	2	55	theme	nectar	188:193	arg1	absence					177:183	the absence	173:183	the absence of nectar	173:193	In the absence of nectar, during certain times of the year or in monocultural landscapes, honey bees forage on honeydew.					
32275718	6	56	with	experiments	832:842	arg1	bees					855:858	caged bees	849:858	caged bees	849:858	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	3	57	theme	Hemiptera	349:357	arg1	herbivores					325:334	different herbivores	315:334	different herbivores of the order Hemiptera that consume phloem sap of plant species	315:398	Honeydew is excreted by different herbivores of the order Hemiptera that consume phloem sap of plant species.					
32275718	4	58	theme	sugars	470:475	arg1	sugars					470:475	sugars	470:475	sugars	470:475	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	58	theme	sugars	470:475	arg1	sugars					492:497	additional sugars	481:497	additional sugars	481:497	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	58	theme	sugars	470:475	arg1	variety					459:465	a higher variety	450:465	a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew	450:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	6	59	theme	called	774:779	arg1	disease					796:802	the so called 'honeydew flow disease'	767:803	the so called 'honeydew flow disease'	767:803	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	7	60	theme	increased	929:937	arg1	uptake					944:949	increased food uptake	929:949	increased food uptake	929:949	Bees fed with melezitose showed increased food uptake, higher gut weights and elevated mortality compared to bees fed a control diet.					
32275718	1	61	theme	Apis	105:108	arg1	mellifera					110:118	Apis mellifera L.	105:121	Apis mellifera L.	105:121	In general, honey bees (Apis mellifera L.) feed on honey produced from collected nectar.					
32275718	1	61	theme	Apis	105:108	arg1	bees					99:102	honey bees	93:102	honey bees (Apis mellifera L.)	93:122	In general, honey bees (Apis mellifera L.) feed on honey produced from collected nectar.					
32275718	8	62	located	observed	1134:1141	arg1	bees					1161:1164	melezitose-fed bees	1146:1164	melezitose-fed bees	1146:1164	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	62	located	observed	1134:1141	arg2	abdomen					1082:1088	swollen abdomen	1074:1088	swollen abdomen	1074:1088	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	62	located	observed	1134:1141	arg2	movement					1120:1127	impaired movement	1111:1127	impaired movement	1111:1127	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	62	located	observed	1134:1141	arg2	tipping					1099:1105	abdomen tipping	1091:1105	abdomen tipping	1091:1105	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	8	62	located	observed	1134:1141	arg2	symptoms					1056:1063	severe disease symptoms	1041:1063	severe disease symptoms	1041:1063	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	7	63	theme	higher	952:957	arg1	weights					963:969	higher gut weights	952:969	higher gut weights	952:969	Bees fed with melezitose showed increased food uptake, higher gut weights and elevated mortality compared to bees fed a control diet.					
32275718	9	64	theme	bacteria	1277:1284	arg1	composition					1246:1256	the species composition	1234:1256	the species composition of the lactic acid bacteria community within the gut microbiota	1234:1320	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	7	65	theme	food	939:942	arg1	uptake					944:949	increased food uptake	929:949	increased food uptake	929:949	Bees fed with melezitose showed increased food uptake, higher gut weights and elevated mortality compared to bees fed a control diet.					
32275718	11	66	theme	melezitose	1637:1646	arg1	capabilities					1659:1670	poor melezitose metabolism capabilities	1632:1670	poor melezitose metabolism capabilities in the intestinal microbiota	1632:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	5	67	theme	melezitose-containing	617:637	arg1	honey					639:643	melezitose-containing honey	617:643	melezitose-containing honey	617:643	However, melezitose-containing honey is known to cause malnutrition in overwintering honey bees.					
32275718	1	68	dep	mellifera	110:118	arg1	L.					120:121	Apis mellifera L.	105:121	Apis mellifera L.	105:121	In general, honey bees (Apis mellifera L.) feed on honey produced from collected nectar.					
32275718	9	69	theme	16S-amplicon	1167:1178	arg1	sequencing					1180:1189	16S-amplicon sequencing	1167:1189	16S-amplicon sequencing	1167:1189	16S-amplicon sequencing indicated that the melezitose diet changed the species composition of the lactic acid bacteria community within the gut microbiota.					
32275718	11	70	theme	metabolism	1648:1657	arg1	capabilities					1659:1670	poor melezitose metabolism capabilities	1632:1670	poor melezitose metabolism capabilities in the intestinal microbiota	1632:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	5	71	theme	honey	693:697	arg1	bees					699:702	honey bees	693:702	honey bees	693:702	However, melezitose-containing honey is known to cause malnutrition in overwintering honey bees.					
32275718	11	72	theme	poor	1632:1635	arg1	capabilities					1659:1670	poor melezitose metabolism capabilities	1632:1670	poor melezitose metabolism capabilities in the intestinal microbiota	1632:1699	Within cages or during winter, when there is no opportunity for excretion, the accumulated melezitose can cause severe intestinal symptoms and death of the bees, probably as result of poor melezitose metabolism capabilities in the intestinal microbiota.					
32275718	4	73	theme	major	577:581	arg1	constituent					583:593	a major constituent	575:593	a major constituent of honeydew	575:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	73	theme	major	577:581	arg1	melezitose					552:561	the trisaccharide melezitose	534:561	the trisaccharide melezitose that can be a major constituent of honeydew	534:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	0	74	theme	intestinal	58:67	arg1	microbiota					69:78	their intestinal microbiota	52:78	their intestinal microbiota	52:78	The trisaccharide melezitose impacts honey bees and their intestinal microbiota.					
32275718	6	75	theme	caged	849:853	arg1	bees					855:858	caged bees	849:858	caged bees	849:858	Following the hypothesis that melezitose may be the cause for the so called 'honeydew flow disease', three independent feeding experiments with caged bees were conducted in consecutive years.					
32275718	2	76	theme	certain	203:209	arg1	times					211:215	certain times	203:215	certain times of the year	203:227	In the absence of nectar, during certain times of the year or in monocultural landscapes, honey bees forage on honeydew.					
32275718	8	77	theme	abdomen	1091:1097	arg1	tipping					1099:1105	abdomen tipping	1091:1105	abdomen tipping	1091:1105	Moreover, severe disease symptoms, such as swollen abdomen, abdomen tipping and impaired movement were observed in melezitose-fed bees.					
32275718	4	78	theme	trisaccharide	538:550	arg1	constituent					583:593	a major constituent	575:593	a major constituent of honeydew	575:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32275718	4	78	theme	trisaccharide	538:550	arg1	melezitose					552:561	the trisaccharide melezitose	534:561	the trisaccharide melezitose that can be a major constituent of honeydew	534:605	In comparison to nectar, honeydew is composed of a higher variety of sugars and additional sugars with higher molecular weight, like the trisaccharide melezitose that can be a major constituent of honeydew.					
32585814	5	0	theme	mixture	795:801	arg1	resolution					779:788	the highest total resolution	761:788	the highest total resolution of a mixture of analytes	761:813	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	5	1	theme	electrolyte	672:682	arg1	composition					642:652	the optimal composition	630:652	the optimal composition of the background electrolyte	630:682	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	6	2	theme	calibration	906:916	arg1	range					918:922	a calibration range	904:922	a calibration range from 0.2 to 5 mmol·L-1	904:945	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	3	theme	calibration	1092:1102	arg1	ranges					1104:1109	the linear calibration ranges	1081:1109	the linear calibration ranges	1081:1109	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	4	theme	repeatability	1010:1022	arg1	detection					958:966	detection	958:966	detection	958:966	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	4	theme	repeatability	1010:1022	arg1	limits					969:974	limits	969:974	limits of quantification, precision, and repeatability	969:1022	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	5	from	mmol·L-1	938:945	arg1	range					918:922	a calibration range	904:922	a calibration range from 0.2 to 5 mmol·L-1	904:945	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	4	6	theme	effective	533:541	arg1	selector					550:557	the most effective chiral selector	524:557	the most effective chiral selector from seven tested cyclodextrin derivates	524:598	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	4	6	theme	effective	533:541	arg1	β-cyclodextrin					493:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	6	7	theme	linear	1085:1090	arg1	ranges					1104:1109	the linear calibration ranges	1081:1109	the linear calibration ranges	1081:1109	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	2	8	from	use	331:333	arg1	medicine					338:345	medicine	338:345	medicine	338:345	The determination of mephedrone and its metabolites is important for understanding its possible use in medicine.					
32585814	3	9	theme	electrophoresis	384:398	arg1	method					364:369	a method	362:369	a method of capillary electrophoresis for the chiral separation of mephedrone	362:438	In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed.					
32585814	3	9	theme	electrophoresis	384:398	arg1	metabolites					448:458	its metabolites	444:458	its metabolites	444:458	In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed.					
32585814	5	10	theme	highest	765:771	arg1	resolution					779:788	the highest total resolution	761:788	the highest total resolution of a mixture of analytes	761:813	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	6	11	dep	5	936:936	arg1	to					933:934	to	933:934	to	933:934	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	12	theme	precision	995:1003	arg1	detection					958:966	detection	958:966	detection	958:966	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	12	theme	precision	995:1003	arg1	limits					969:974	limits	969:974	limits of quantification, precision, and repeatability	969:1022	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	5	13	theme	total	773:777	arg1	resolution					779:788	the highest total resolution	761:788	the highest total resolution of a mixture of analytes	761:813	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	4	14	theme	Carboxymethylated	475:491	arg1	β-cyclodextrin					493:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	4	14	theme	Carboxymethylated	475:491	arg1	selector					550:557	the most effective chiral selector	524:557	the most effective chiral selector from seven tested cyclodextrin derivates	524:598	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	5	15	theme	optimal	634:640	arg1	composition					642:652	the optimal composition	630:652	the optimal composition of the background electrolyte	630:682	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	0	16	theme	Chiral	122:127	arg1	Cyclodextrins					105:117	Cyclodextrins	105:117	Cyclodextrins	105:117	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	0	16	theme	Chiral	122:127	arg1	Selectors					129:137	Chiral Selectors	122:137	Chiral Selectors	122:137	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	5	17	theme	analytes	806:813	arg1	mixture					795:801	a mixture	793:801	a mixture of analytes	793:813	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	2	18	theme	metabolites	275:285	arg1	important					290:298	important	290:298	important	290:298	The determination of mephedrone and its metabolites is important for understanding its possible use in medicine.					
32585814	2	18	theme	metabolites	275:285	arg1	determination					239:251	The determination	235:251	The determination of mephedrone and its metabolites	235:285	The determination of mephedrone and its metabolites is important for understanding its possible use in medicine.					
32585814	3	19	theme	capillary	374:382	arg1	electrophoresis					384:398	capillary electrophoresis	374:398	capillary electrophoresis	374:398	In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed.					
32585814	1	20	attach	derived	176:182	arg2	compound					167:174	a psychoactive compound	152:174	a psychoactive compound derived from cathinone	152:197	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	1	20	attach	derived	176:182	arg1	cathinone					189:197	cathinone	189:197	cathinone	189:197	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	1	20	attach	derived	176:182	arg2	Mephedrone					140:149	Mephedrone	140:149	Mephedrone	140:149	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	4	21	from	derivates	590:598	arg1	selector					550:557	the most effective chiral selector	524:557	the most effective chiral selector from seven tested cyclodextrin derivates	524:598	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	4	21	from	derivates	590:598	arg1	β-cyclodextrin					493:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	4	22	theme	cyclodextrin	577:588	arg1	derivates					590:598	seven tested cyclodextrin derivates	564:598	seven tested cyclodextrin derivates	564:598	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	5	23	theme	background	661:670	arg1	electrolyte					672:682	the background electrolyte	657:682	the background electrolyte	657:682	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	2	24	theme	possible	322:329	arg1	use					331:333	its possible use	318:333	its possible use in medicine	318:345	The determination of mephedrone and its metabolites is important for understanding its possible use in medicine.					
32585814	3	25	theme	mephedrone	429:438	arg1	separation					415:424	the chiral separation	404:424	the chiral separation of mephedrone	404:438	In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed.					
32585814	4	26	theme	tested	570:575	arg1	derivates					590:598	seven tested cyclodextrin derivates	564:598	seven tested cyclodextrin derivates	564:598	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	5	27	theme	simplex	614:620	arg1	method					622:627	the simplex method	610:627	the simplex method	610:627	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	5	28	theme	mmol·L-1	719:726	arg1	β-cyclodextrin					746:759	7.5 mmol·L-1 carboxymethylated β-cyclodextrin	715:759	7.5 mmol·L-1 carboxymethylated β-cyclodextrin	715:759	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	3	29	theme	chiral	408:413	arg1	separation					415:424	the chiral separation	404:424	the chiral separation of mephedrone	404:438	In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed.					
32585814	5	30	theme	carboxymethylated	728:744	arg1	β-cyclodextrin					746:759	7.5 mmol·L-1 carboxymethylated β-cyclodextrin	715:759	7.5 mmol·L-1 carboxymethylated β-cyclodextrin	715:759	Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved.					
32585814	6	31	theme	fitting	1067:1073	arg1	test					1075:1078	Mandel's fitting test	1058:1078	Mandel's fitting test	1058:1078	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	0	32	theme	Mephedrone	39:48	arg1	Determination					22:34	Determination	22:34	Determination	22:34	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	0	32	theme	Mephedrone	39:48	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation	0:16	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	1	33	theme	designer	220:227	arg1	drug					229:232	a designer drug	218:232	a designer drug	218:232	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	1	33	theme	designer	220:227	arg1	Mephedrone					140:149	Mephedrone	140:149	Mephedrone	140:149	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	0	34	theme	Metabolites	58:68	arg1	Determination					22:34	Determination	22:34	Determination	22:34	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	0	34	theme	Metabolites	58:68	arg1	Enantioseparation					0:16	Enantioseparation	0:16	Enantioseparation	0:16	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	4	35	theme	chiral	543:548	arg1	selector					550:557	the most effective chiral selector	524:557	the most effective chiral selector from seven tested cyclodextrin derivates	524:598	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	4	35	theme	chiral	543:548	arg1	β-cyclodextrin					493:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin	475:506	Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates.					
32585814	6	36	theme	quantification	979:992	arg1	detection					958:966	detection	958:966	detection	958:966	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	6	36	theme	quantification	979:992	arg1	limits					969:974	limits	969:974	limits of quantification, precision, and repeatability	969:1022	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	1	37	used	used	210:213	arg2	drug					229:232	a designer drug	218:232	a designer drug	218:232	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	1	37	used	used	210:213	arg2	compound					167:174	a psychoactive compound	152:174	a psychoactive compound derived from cathinone	152:197	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	1	37	used	used	210:213	arg2	Mephedrone					140:149	Mephedrone	140:149	Mephedrone	140:149	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	6	38	theme	calibration	865:875	arg1	curves					877:882	calibration curves	865:882	calibration curves	865:882	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
32585814	0	39	theme	Capillary	73:81	arg1	Electrophoresis					83:97	Capillary Electrophoresis	73:97	Capillary Electrophoresis	73:97	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors.					
32585814	2	40	theme	mephedrone	256:265	arg1	important					290:298	important	290:298	important	290:298	The determination of mephedrone and its metabolites is important for understanding its possible use in medicine.					
32585814	2	40	theme	mephedrone	256:265	arg1	determination					239:251	The determination	235:251	The determination of mephedrone and its metabolites	235:285	The determination of mephedrone and its metabolites is important for understanding its possible use in medicine.					
32585814	1	41	theme	psychoactive	154:165	arg1	compound					167:174	a psychoactive compound	152:174	a psychoactive compound derived from cathinone	152:197	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	1	41	theme	psychoactive	154:165	arg1	Mephedrone					140:149	Mephedrone	140:149	Mephedrone	140:149	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug.					
32585814	6	42	theme	detection	958:966	arg1	limits					948:953	limits	948:953	limits of detection, limits of quantification, precision, and repeatability	948:1022	For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.					
33781815	7	0	theme	nano-ZnO-containing	1170:1188	arg1	beads					1203:1207	The prepared nano-ZnO-containing alginate/PVA beads	1157:1207	The prepared nano-ZnO-containing alginate/PVA beads	1157:1207	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	7	0	theme	nano-ZnO-containing	1170:1188	arg1	attractive					1216:1225	attractive	1216:1225	attractive	1216:1225	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	4	1	theme	ZnAlg	699:703	arg1	beads					705:709	The pure CaAlg and ZnAlg beads	680:709	The pure CaAlg and ZnAlg beads	680:709	The pure CaAlg and ZnAlg beads demonstrated a poor water absorption and retention, which were strongly enhanced by the incorporation of PVA into the beads.					
33781815	2	2	dep	entrapped	310:318	arg1	reduce					264:269	reduce	264:269	To reduce their dispersion in the environment	261:305	To reduce their dispersion in the environment we entrapped nano-ZnO in biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA).					
33781815	7	3	theme	alginate/PVA	1190:1201	arg1	beads					1203:1207	The prepared nano-ZnO-containing alginate/PVA beads	1157:1207	The prepared nano-ZnO-containing alginate/PVA beads	1157:1207	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	7	3	theme	alginate/PVA	1190:1201	arg1	attractive					1216:1225	attractive	1216:1225	attractive	1216:1225	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	5	4	theme	sand	880:883	arg1	column					885:890	a sand column	878:890	a sand column	878:890	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	5	5	theme	Ca	1030:1031	arg1	alginates					1033:1041	Ca alginates	1030:1041	Ca alginates	1030:1041	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	3	6	theme	alginate	550:557	arg1	ion					572:574	alginate crosslinking ion	550:574	alginate crosslinking ion	550:574	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	5	7	theme	Zn-crosslinked	919:932	arg1	beads					934:938	the Zn-crosslinked beads	915:938	the Zn-crosslinked beads	915:938	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	0	8	theme	fertilizer	98:107	arg1	applications					109:120	fertilizer applications	98:120	fertilizer applications	98:120	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	3	9	theme	crosslinking	559:570	arg1	ion					572:574	alginate crosslinking ion	550:574	alginate crosslinking ion	550:574	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	2	10	theme	polyvinyl	387:395	arg1	PVA					406:408	PVA	406:408	PVA	406:408	To reduce their dispersion in the environment we entrapped nano-ZnO in biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA).					
33781815	2	10	theme	polyvinyl	387:395	arg1	alcohol					397:403	polyvinyl alcohol	387:403	polyvinyl alcohol (PVA)	387:409	To reduce their dispersion in the environment we entrapped nano-ZnO in biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA).					
33781815	5	11	theme	Zn	851:852	arg1	release					854:860	The continuous Zn release	836:860	The continuous Zn release	836:860	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	5	12	theme	gradual	1057:1063	arg1	release					1068:1074	only a gradual Zn release	1050:1074	only a gradual Zn release	1050:1074	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	1	13	theme	fertilizer	177:186	arg1	materials					188:196	fertilizer materials	177:196	fertilizer materials	177:196	Zinc oxide nanoparticles (nano-ZnO) are attractive as fertilizer materials but high concentrations may negatively affect the environment.					
33781815	5	14	theme	Zn	1065:1066	arg1	release					1068:1074	only a gradual Zn release	1050:1074	only a gradual Zn release	1050:1074	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	3	15	theme	ionotropic	457:466	arg1	gelation					468:475	ionotropic gelation	457:475	ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+)	457:529	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	5	16	theme	high	957:960	arg1	concentrations					962:975	high concentrations	957:975	high concentrations of Zn followed by a more gradual Zn release	957:1019	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	5	17	theme	Zn	980:981	arg1	concentrations					962:975	high concentrations	957:975	high concentrations of Zn followed by a more gradual Zn release	957:1019	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	2	18	theme	polymer	346:352	arg1	beads					354:358	biodegradable polymer beads	332:358	biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA)	332:409	To reduce their dispersion in the environment we entrapped nano-ZnO in biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA).					
33781815	2	19	theme	biodegradable	332:344	arg1	beads					354:358	biodegradable polymer beads	332:358	biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA)	332:409	To reduce their dispersion in the environment we entrapped nano-ZnO in biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA).					
33781815	1	20	theme	high	202:205	arg1	concentrations					207:220	high concentrations	202:220	high concentrations	202:220	Zinc oxide nanoparticles (nano-ZnO) are attractive as fertilizer materials but high concentrations may negatively affect the environment.					
33781815	4	21	theme	water	731:735	arg1	absorption					737:746	a poor water absorption	724:746	a poor water absorption	724:746	The pure CaAlg and ZnAlg beads demonstrated a poor water absorption and retention, which were strongly enhanced by the incorporation of PVA into the beads.					
33781815	5	22	theme	gradual	1002:1008	arg1	release					1013:1019	a more gradual Zn release	995:1019	a more gradual Zn release	995:1019	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	3	23	theme	ion	572:574	arg1	effect					540:545	the effect	536:545	the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release	536:660	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	24	theme	crosslinking	497:508	arg1	Zn2+					525:528	Zn2+	525:528	Zn2+	525:528	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	24	theme	crosslinking	497:508	arg1	Ca2+					516:519	Ca2+	516:519	Ca2+	516:519	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	24	theme	crosslinking	497:508	arg1	ions					510:513	two different crosslinking ions	483:513	two different crosslinking ions (Ca2+ and Zn2+)	483:529	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	0	25	theme	nano-ZnO	14:21	arg1	Entrapment					0:9	Entrapment	0:9	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.	0:121	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	6	26	theme	ion	1140:1142	arg1	composition					1144:1154	the crosslinking ion composition	1123:1154	the crosslinking ion composition	1123:1154	The Zn dissolution kinetics could be tuned by the crosslinking ion composition.					
33781815	3	27	theme	zinc	649:652	arg1	release					654:660	zinc release	649:660	zinc release	649:660	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	1	28	theme	Zinc	123:126	arg1	oxide					128:132	Zinc oxide	123:132	Zinc oxide nanoparticles (nano-ZnO)	123:157	Zinc oxide nanoparticles (nano-ZnO) are attractive as fertilizer materials but high concentrations may negatively affect the environment.					
33781815	0	29	theme	alcohol	47:53	arg1	beads					55:59	alginate/polyvinyl alcohol beads	28:59	alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications	28:120	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	7	30	theme	water-limited	1264:1276	arg1	conditions					1278:1287	water-limited conditions	1264:1287	water-limited conditions	1264:1287	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	4	31	theme	PVA	816:818	arg1	incorporation					799:811	the incorporation	795:811	the incorporation of PVA into the beads	795:833	The pure CaAlg and ZnAlg beads demonstrated a poor water absorption and retention, which were strongly enhanced by the incorporation of PVA into the beads.					
33781815	2	32	from	dispersion	277:286	arg1	environment					295:305	the environment	291:305	the environment	291:305	To reduce their dispersion in the environment we entrapped nano-ZnO in biodegradable polymer beads consisting of alginate and polyvinyl alcohol (PVA).					
33781815	0	33	theme	alginate/polyvinyl	28:45	arg1	beads					55:59	alginate/polyvinyl alcohol beads	28:59	alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications	28:120	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	3	34	theme	different	487:495	arg1	Zn2+					525:528	Zn2+	525:528	Zn2+	525:528	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	34	theme	different	487:495	arg1	Ca2+					516:519	Ca2+	516:519	Ca2+	516:519	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	34	theme	different	487:495	arg1	ions					510:513	two different crosslinking ions	483:513	two different crosslinking ions (Ca2+ and Zn2+)	483:529	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	35	theme	alginate/PVA/ZnO	416:431	arg1	beads					433:437	The alginate/PVA/ZnO beads	412:437	The alginate/PVA/ZnO beads	412:437	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	5	36	link	Zn-crosslinked	919:932	arg1	beads					934:938	the Zn-crosslinked beads	915:938	the Zn-crosslinked beads	915:938	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	1	37	theme	oxide	128:132	arg1	nano-ZnO					149:156	nano-ZnO	149:156	nano-ZnO	149:156	Zinc oxide nanoparticles (nano-ZnO) are attractive as fertilizer materials but high concentrations may negatively affect the environment.					
33781815	1	37	theme	oxide	128:132	arg1	nanoparticles					134:146	Zinc oxide nanoparticles	123:146	Zinc oxide nanoparticles (nano-ZnO)	123:157	Zinc oxide nanoparticles (nano-ZnO) are attractive as fertilizer materials but high concentrations may negatively affect the environment.					
33781815	6	38	theme	crosslinking	1127:1138	arg1	composition					1144:1154	the crosslinking ion composition	1123:1154	the crosslinking ion composition	1123:1154	The Zn dissolution kinetics could be tuned by the crosslinking ion composition.					
33781815	5	39	theme	Zn	1010:1011	arg1	release					1013:1019	a more gradual Zn release	995:1019	a more gradual Zn release	995:1019	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	3	40	theme	bead	595:598	arg1	structure					600:608	bead structure	595:608	bead structure	595:608	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	6	41	theme	Zn	1081:1082	arg1	kinetics					1096:1103	The Zn dissolution kinetics	1077:1103	The Zn dissolution kinetics	1077:1103	The Zn dissolution kinetics could be tuned by the crosslinking ion composition.					
33781815	3	42	theme	PVA	580:582	arg1	content					584:590	PVA content	580:590	PVA content	580:590	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	7	43	theme	Zn	1231:1232	arg1	applications					1245:1256	Zn fertilizer applications	1231:1256	Zn fertilizer applications under water-limited conditions	1231:1287	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	4	44	theme	poor	726:729	arg1	absorption					737:746	a poor water absorption	724:746	a poor water absorption	724:746	The pure CaAlg and ZnAlg beads demonstrated a poor water absorption and retention, which were strongly enhanced by the incorporation of PVA into the beads.					
33781815	3	45	theme	water	629:633	arg1	retention					635:643	water retention	629:643	water retention	629:643	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	4	46	theme	pure	684:687	arg1	beads					705:709	The pure CaAlg and ZnAlg beads	680:709	The pure CaAlg and ZnAlg beads	680:709	The pure CaAlg and ZnAlg beads demonstrated a poor water absorption and retention, which were strongly enhanced by the incorporation of PVA into the beads.					
33781815	6	47	theme	dissolution	1084:1094	arg1	kinetics					1096:1103	The Zn dissolution kinetics	1077:1103	The Zn dissolution kinetics	1077:1103	The Zn dissolution kinetics could be tuned by the crosslinking ion composition.					
33781815	7	48	theme	fertilizer	1234:1243	arg1	applications					1245:1256	Zn fertilizer applications	1231:1256	Zn fertilizer applications under water-limited conditions	1231:1287	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	0	49	theme	crosslinking	76:87	arg1	ions					89:92	different crosslinking ions	66:92	different crosslinking ions for fertilizer applications	66:120	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	5	50	theme	continuous	840:849	arg1	release					854:860	The continuous Zn release	836:860	The continuous Zn release	836:860	The continuous Zn release was measured in a sand column, and it was found that the Zn-crosslinked beads rapidly released high concentrations of Zn followed by a more gradual Zn release, whereas Ca alginates showed only a gradual Zn release.					
33781815	3	51	theme	water	611:615	arg1	absorption					617:626	water absorption	611:626	water absorption	611:626	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	0	52	with	beads	55:59	arg1	ions					89:92	different crosslinking ions	66:92	different crosslinking ions for fertilizer applications	66:120	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	4	53	theme	CaAlg	689:693	arg1	beads					705:709	The pure CaAlg and ZnAlg beads	680:709	The pure CaAlg and ZnAlg beads	680:709	The pure CaAlg and ZnAlg beads demonstrated a poor water absorption and retention, which were strongly enhanced by the incorporation of PVA into the beads.					
33781815	0	54	theme	different	66:74	arg1	ions					89:92	different crosslinking ions	66:92	different crosslinking ions for fertilizer applications	66:120	Entrapment of nano-ZnO into alginate/polyvinyl alcohol beads with different crosslinking ions for fertilizer applications.					
33781815	3	55	dep	ions	510:513	arg1	Zn2+					525:528	Zn2+	525:528	Zn2+	525:528	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	55	dep	ions	510:513	arg1	Ca2+					516:519	Ca2+	516:519	Ca2+	516:519	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	55	dep	ions	510:513	arg1	ions					510:513	two different crosslinking ions	483:513	two different crosslinking ions (Ca2+ and Zn2+)	483:529	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	56	theme	content	584:590	arg1	effect					540:545	the effect	536:545	the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release	536:660	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	57	from	effect	540:545	arg1	structure					600:608	bead structure	595:608	bead structure	595:608	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	57	from	effect	540:545	arg1	absorption					617:626	water absorption	611:626	water absorption	611:626	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	57	from	effect	540:545	arg1	retention					635:643	water retention	629:643	water retention	629:643	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	3	57	from	effect	540:545	arg1	release					654:660	zinc release	649:660	zinc release	649:660	The alginate/PVA/ZnO beads were prepared via ionotropic gelation using two different crosslinking ions (Ca2+ and Zn2+), and the effect of alginate crosslinking ion and PVA content on bead structure, water absorption, water retention and zinc release was investigated.					
33781815	7	58	theme	prepared	1161:1168	arg1	beads					1203:1207	The prepared nano-ZnO-containing alginate/PVA beads	1157:1207	The prepared nano-ZnO-containing alginate/PVA beads	1157:1207	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33781815	7	58	theme	prepared	1161:1168	arg1	attractive					1216:1225	attractive	1216:1225	attractive	1216:1225	The prepared nano-ZnO-containing alginate/PVA beads may be attractive for Zn fertilizer applications under water-limited conditions.					
33627574	6	0	theme	significant	1415:1425	arg1	difference					1427:1436	no significant difference	1412:1436	no significant difference	1412:1436	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	7	1	theme	solifenacin	1650:1660	arg1	succinate					1662:1670	solifenacin succinate	1650:1670	solifenacin succinate	1650:1670	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	3	2	theme	ODTs	822:825	arg1	preparation					807:817	the preparation	803:817	the preparation of ODTs containing PEGS	803:841	For the preparation of ODTs containing PEGS, a semi-direct compression method was adopted in order to prevent damage to the PEGS by processes such as granulation or compaction.					
33627574	2	3	theme	drug	514:517	arg1	release					519:525	initial drug release	506:525	initial drug release from the prepared PEGS	506:548	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	5	4	theme	compression	1383:1393	arg1	force					1395:1399	a low compression force	1377:1399	a low compression force	1377:1399	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	4	5	theme	other	1150:1154	arg1	additives					1156:1164	other additives	1150:1164	other additives	1150:1164	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	4	6	theme	fibre-shaped	989:1000	arg1	cellulose					1019:1027	a fibre-shaped microcrystalline cellulose	987:1027	a fibre-shaped microcrystalline cellulose	987:1027	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	7	7	contain	containing	1567:1576	arg2	PEGS					1578:1581	PEGS	1578:1581	PEGS	1578:1581	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	7	7	contain	containing	1567:1576	arg1	ODTs					1562:1565	The ODTs	1558:1565	The ODTs containing PEGS	1558:1581	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	2	8	theme	in	478:479	arg1	tests					499:503	in vitro dissolution tests	478:503	in vitro dissolution tests	478:503	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	9	theme	unpleasant	340:349	arg1	taste					351:355	the unpleasant taste	336:355	the unpleasant taste of the drug	336:367	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	6	10	contain	containing	1500:1509	arg1	ODTs					1495:1498	the optimally formulated ODTs	1470:1498	the optimally formulated ODTs containing PEGS tableted at different compression forces	1470:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	6	10	contain	containing	1500:1509	arg2	PEGS					1511:1514	PEGS	1511:1514	PEGS tableted at different compression forces	1511:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	6	11	theme	drug-release	1445:1456	arg1	profiles					1458:1465	the drug-release profiles	1441:1465	the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces	1441:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	2	12	theme	layer	751:755	arg1	thickness					702:710	the thickness	698:710	particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer	687:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	13	theme	gelling-swelling	267:282	arg1	PEGS					291:294	PEGS	291:294	PEGS	291:294	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	1	13	theme	gelling-swelling	267:282	arg1	layer					284:288	a gelling-swelling layer	265:288	a gelling-swelling layer (PEGS)	265:295	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	2	14	theme	dissolution	487:497	arg1	tests					499:503	in vitro dissolution tests	478:503	in vitro dissolution tests	478:503	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	15	theme	taste	351:355	arg1	taste-masking					319:331	taste-masking	319:331	taste-masking	319:331	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	5	16	dep	hardness	1271:1278	arg1	properties					1305:1314	properties	1305:1314	properties	1305:1314	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	4	17	theme	content	1061:1067	arg1	uniformity					1069:1078	the content uniformity	1057:1078	the content uniformity of the ODTs	1057:1090	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	5	18	theme	high	1266:1269	arg1	hardness					1271:1278	relatively high hardness	1255:1278	relatively high hardness	1255:1278	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	7	19	theme	succinate	1662:1670	arg1	taste-masking					1633:1645	taste-masking	1633:1645	taste-masking of solifenacin succinate	1633:1670	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	0	20	from	Embedded	58:65	arg1	Layer					89:93	a Gelling-Swelling Layer	70:93	a Gelling-Swelling Layer (PEGS)	70:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	0	20	from	Embedded	58:65	arg1	PEGS					96:99	PEGS	96:99	PEGS	96:99	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	2	21	theme	water-insoluble	774:788	arg1	polymer					790:796	a water-insoluble polymer	772:796	a water-insoluble polymer	772:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	4	22	theme	cellulose	1019:1027	arg1	use					980:982	The use	976:982	The use of a fibre-shaped microcrystalline cellulose with poor fluidity	976:1046	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	0	23	theme	Embedded	58:65	arg1	Particles					48:56	Solifenacin Succinate Functional Particles	15:56	Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS)	15:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	5	24	with	mannitol	1188:1195	arg1	structure					1211:1219	a hollow structure	1202:1219	a hollow structure produced by spray drying	1202:1244	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	4	25	with	use	980:982	arg1	fluidity					1039:1046	poor fluidity	1034:1046	poor fluidity	1034:1046	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	2	26	theme	PEGS	678:681	arg1	composition					659:669	the composition	655:669	the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer	655:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	27	theme	drug	364:367	arg1	taste					351:355	the unpleasant taste	336:355	the unpleasant taste of the drug	336:367	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	2	28	contain	contains	763:770	arg2	polymer					790:796	a water-insoluble polymer	772:796	a water-insoluble polymer	772:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	2	28	contain	contains	763:770	arg1	layer					751:755	the outer water-penetration control layer	715:755	the outer water-penetration control layer which contains a water-insoluble polymer	715:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	29	theme	disintegrating	444:457	arg1	ODTs					468:471	ODTs	468:471	ODTs	468:471	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	1	29	theme	disintegrating	444:457	arg1	tablets					459:465	orally disintegrating tablets	437:465	orally disintegrating tablets (ODTs)	437:472	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	5	30	theme	hollow	1204:1209	arg1	structure					1211:1219	a hollow structure	1202:1219	a hollow structure produced by spray drying	1202:1244	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	2	31	theme	prepared	536:543	arg1	PEGS					545:548	the prepared PEGS	532:548	the prepared PEGS	532:548	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	32	theme	research	178:185	arg1	purpose					162:168	The purpose	158:168	The purpose of this research	158:185	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	2	33	theme	initial	506:512	arg1	release					519:525	initial drug release	506:525	initial drug release from the prepared PEGS	506:548	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	6	34	theme	different	1528:1536	arg1	forces					1550:1555	different compression forces	1528:1555	different compression forces	1528:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	4	35	theme	poor	1034:1037	arg1	fluidity					1039:1046	poor fluidity	1034:1046	poor fluidity	1034:1046	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	1	36	theme	rapid	373:377	arg1	elution					384:390	both taste-masking of the unpleasant taste of the drug and rapid drug elution	314:390	both taste-masking of the unpleasant taste of the drug and rapid drug elution	314:390	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	0	37	theme	Succinate	27:35	arg1	Particles					48:56	Solifenacin Succinate Functional Particles	15:56	Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS)	15:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	2	38	dep	in	478:479	arg1	vitro					481:485	vitro	481:485	vitro	481:485	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	6	39	theme	compression	1538:1548	arg1	forces					1550:1555	different compression forces	1528:1555	different compression forces	1528:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	3	40	contain	containing	827:836	arg2	PEGS					838:841	PEGS	838:841	PEGS	838:841	For the preparation of ODTs containing PEGS, a semi-direct compression method was adopted in order to prevent damage to the PEGS by processes such as granulation or compaction.					
33627574	3	40	contain	containing	827:836	arg1	ODTs					822:825	ODTs	822:825	ODTs containing PEGS	822:841	For the preparation of ODTs containing PEGS, a semi-direct compression method was adopted in order to prevent damage to the PEGS by processes such as granulation or compaction.					
33627574	0	41	theme	Solifenacin	15:25	arg1	Particles					48:56	Solifenacin Succinate Functional Particles	15:56	Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS)	15:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	1	42	from	layer	284:288	arg1	embedded					253:260	embedded	253:260	embedded	253:260	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	5	43	theme	spray	1233:1237	arg1	drying					1239:1244	spray drying	1233:1244	spray drying	1233:1244	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	3	44	theme	semi-direct	846:856	arg1	method					870:875	a semi-direct compression method	844:875	a semi-direct compression method	844:875	For the preparation of ODTs containing PEGS, a semi-direct compression method was adopted in order to prevent damage to the PEGS by processes such as granulation or compaction.					
33627574	7	45	theme	lag	1609:1611	arg1	time					1613:1616	a drug-release lag time	1594:1616	a drug-release lag time sufficient for taste-masking of solifenacin succinate	1594:1670	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	0	46	theme	Particles	48:56	arg1	Preparation					0:10	Preparation	0:10	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS)	0:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	0	46	theme	Particles	48:56	arg1	Formulation					112:122	Their Formulation	106:122	Their Formulation in Orally Disintegrating Tablets	106:155	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	0	47	theme	Disintegrating	134:147	arg1	Tablets					149:155	Orally Disintegrating Tablets	127:155	Orally Disintegrating Tablets	127:155	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	0	48	from	Layer	89:93	arg1	Embedded					58:65	Embedded	58:65	Embedded	58:65	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	2	49	from	PEGS	678:681	arg1	particular					687:696	particular	687:696	particular	687:696	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	3	50	theme	compression	858:868	arg1	method					870:875	a semi-direct compression method	844:875	a semi-direct compression method	844:875	For the preparation of ODTs containing PEGS, a semi-direct compression method was adopted in order to prevent damage to the PEGS by processes such as granulation or compaction.					
33627574	5	51	contain	containing	1334:1343	arg1	ODTs					1329:1332	the final ODTs	1319:1332	the final ODTs containing PEGS, which were tableted using a low compression force	1319:1399	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	5	51	contain	containing	1334:1343	arg2	PEGS					1345:1348	PEGS	1345:1348	PEGS	1345:1348	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	0	52	theme	Functional	37:46	arg1	Particles					48:56	Solifenacin Succinate Functional Particles	15:56	Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS)	15:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	7	53	theme	drug-release	1596:1607	arg1	time					1613:1616	a drug-release lag time	1594:1616	a drug-release lag time sufficient for taste-masking of solifenacin succinate	1594:1670	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	1	54	theme	drug	379:382	arg1	elution					384:390	both taste-masking of the unpleasant taste of the drug and rapid drug elution	314:390	both taste-masking of the unpleasant taste of the drug and rapid drug elution	314:390	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	6	55	theme	formulated	1484:1493	arg1	ODTs					1495:1498	the optimally formulated ODTs	1470:1498	the optimally formulated ODTs containing PEGS tableted at different compression forces	1470:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	4	56	theme	crystal	1100:1106	arg1	fibres					1108:1113	the crystal fibres	1096:1113	the crystal fibres	1096:1113	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	1	57	theme	solifenacin	210:220	arg1	particles					243:251	solifenacin succinate functional particles	210:251	solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS)	210:295	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	5	58	theme	spherical	1178:1186	arg1	mannitol					1188:1195	spherical mannitol	1178:1195	spherical mannitol with a hollow structure produced by spray drying	1178:1244	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	2	59	dep	particular	687:696	arg1	thickness					702:710	the thickness	698:710	particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer	687:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	0	60	from	Preparation	0:10	arg1	Tablets					149:155	Orally Disintegrating Tablets	127:155	Orally Disintegrating Tablets	127:155	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	1	61	from	embedded	253:260	arg1	PEGS					291:294	PEGS	291:294	PEGS	291:294	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	1	61	from	embedded	253:260	arg1	layer					284:288	a gelling-swelling layer	265:288	a gelling-swelling layer (PEGS)	265:295	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	5	62	theme	mannitol	1188:1195	arg1	use					1171:1173	The use	1167:1173	The use of spherical mannitol with a hollow structure produced by spray drying	1167:1244	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	5	63	theme	low	1379:1381	arg1	force					1395:1399	a low compression force	1377:1399	a low compression force	1377:1399	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	2	64	theme	control	743:749	arg1	layer					751:755	the outer water-penetration control layer	715:755	the outer water-penetration control layer which contains a water-insoluble polymer	715:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	5	65	theme	final	1323:1327	arg1	ODTs					1329:1332	the final ODTs	1319:1332	the final ODTs containing PEGS, which were tableted using a low compression force	1319:1399	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	2	66	theme	water-penetration	725:741	arg1	layer					751:755	the outer water-penetration control layer	715:755	the outer water-penetration control layer which contains a water-insoluble polymer	715:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	67	theme	succinate	222:230	arg1	particles					243:251	solifenacin succinate functional particles	210:251	solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS)	210:295	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	7	68	theme	sufficient	1618:1627	arg1	time					1613:1616	a drug-release lag time	1594:1616	a drug-release lag time sufficient for taste-masking of solifenacin succinate	1594:1670	The ODTs containing PEGS maintained a drug-release lag time sufficient for taste-masking of solifenacin succinate.					
33627574	2	69	from	particular	687:696	arg1	composition					659:669	the composition	655:669	the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer	655:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	4	70	theme	ODTs	1087:1090	arg1	uniformity					1069:1078	the content uniformity	1057:1078	the content uniformity of the ODTs	1057:1090	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	2	71	theme	outer	719:723	arg1	layer					751:755	the outer water-penetration control layer	715:755	the outer water-penetration control layer which contains a water-insoluble polymer	715:796	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	72	theme	functional	232:241	arg1	particles					243:251	solifenacin succinate functional particles	210:251	solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS)	210:295	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	0	73	theme	Gelling-Swelling	72:87	arg1	Layer					89:93	a Gelling-Swelling Layer	70:93	a Gelling-Swelling Layer (PEGS)	70:100	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	0	73	theme	Gelling-Swelling	72:87	arg1	PEGS					96:99	PEGS	96:99	PEGS	96:99	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	2	74	from	composition	659:669	arg1	particular					687:696	particular	687:696	particular	687:696	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	6	75	theme	ODTs	1495:1498	arg1	profiles					1458:1465	the drug-release profiles	1441:1465	the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces	1441:1555	There was no significant difference in the drug-release profiles of the optimally formulated ODTs containing PEGS tableted at different compression forces.					
33627574	4	76	theme	microcrystalline	1002:1017	arg1	cellulose					1019:1027	a fibre-shaped microcrystalline cellulose	987:1027	a fibre-shaped microcrystalline cellulose	987:1027	The use of a fibre-shaped microcrystalline cellulose with poor fluidity improved the content uniformity of the ODTs, as the crystal fibres became entangled with the PEGS and other additives.					
33627574	2	77	from	PEGS	545:548	arg1	release					519:525	initial drug release	506:525	initial drug release from the prepared PEGS	506:548	In in vitro dissolution tests, initial drug release from the prepared PEGS could be suppressed to less than 1% after 2 min and increased to more than 85% after 30 min by adjusting the composition of the PEGS, in particular the thickness of the outer water-penetration control layer which contains a water-insoluble polymer.					
33627574	1	78	theme	taste-masking	319:331	arg1	elution					384:390	both taste-masking of the unpleasant taste of the drug and rapid drug elution	314:390	both taste-masking of the unpleasant taste of the drug and rapid drug elution	314:390	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
33627574	0	79	from	Formulation	112:122	arg1	Tablets					149:155	Orally Disintegrating Tablets	127:155	Orally Disintegrating Tablets	127:155	Preparation of Solifenacin Succinate Functional Particles Embedded in a Gelling-Swelling Layer (PEGS) and Their Formulation in Orally Disintegrating Tablets.					
33627574	5	80	theme	rapid	1284:1288	arg1	disintegration					1290:1303	rapid disintegration	1284:1303	rapid disintegration	1284:1303	The use of spherical mannitol with a hollow structure produced by spray drying imparted relatively high hardness and rapid disintegration properties to the final ODTs containing PEGS, which were tableted using a low compression force.					
33627574	1	81	theme	embedded	253:260	arg1	particles					243:251	solifenacin succinate functional particles	210:251	solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS)	210:295	The purpose of this research was firstly to prepare solifenacin succinate functional particles embedded in a gelling-swelling layer (PEGS) so as to achieve both taste-masking of the unpleasant taste of the drug and rapid drug elution, and secondly to incorporate these PEGS into orally disintegrating tablets (ODTs).					
34679697	2	0	theme	biological	414:423	arg1	activities					425:434	biological activities	414:434	biological activities	414:434	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	6	1	theme	tea	1018:1020	arg1	polysaccharides					1022:1036	Pu-erh tea polysaccharides	1011:1036	Pu-erh tea polysaccharides	1011:1036	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	7	2	theme	phenolic	1412:1419	arg1	compounds					1421:1429	phenolic compounds	1412:1429	phenolic compounds	1412:1429	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	0	3	from	Characteristics	11:25	arg1	Teas					77:80	12 Selected Chinese Teas	57:80	12 Selected Chinese Teas	57:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	3	4	contain	had	627:629	arg2	yield					654:658	the highest extraction yield	631:658	the highest extraction yield (6.38 ± 0.28%)	631:673	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	4	contain	had	627:629	arg2	%					672:672	6.38 ± 0.28%	661:672	6.38 ± 0.28%	661:672	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	4	contain	had	627:629	arg1	polysaccharides					611:625	Pu-erh tea polysaccharides	600:625	Pu-erh tea polysaccharides	600:625	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	0	5	theme	Antioxidant	93:103	arg1	Activities					123:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	6	6	theme	antioxidant	1130:1140	arg1	activities					1160:1169	the best antioxidant and anti-diabetic activities	1121:1169	the best antioxidant and anti-diabetic activities	1121:1169	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	8	7	theme	structural-function	1565:1583	arg1	relationship					1585:1596	the structural-function relationship	1561:1596	the structural-function relationship of crude tea polysaccharides	1561:1625	The conclusion from this study can help understand the structural-function relationship of crude tea polysaccharides.					
34679697	1	8	theme	oolong	242:247	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	8	theme	oolong	242:247	arg1	tea					249:251	oolong tea	242:251	oolong tea	242:251	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	7	9	theme	anti-diabetic	1354:1366	arg1	activities					1368:1377	their antioxidant and anti-diabetic activities	1332:1377	their antioxidant and anti-diabetic activities	1332:1377	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	3	10	theme	tea	607:609	arg1	polysaccharides					611:625	Pu-erh tea polysaccharides	600:625	Pu-erh tea polysaccharides	600:625	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	5	11	theme	obvious	896:902	arg1	activities					985:994	obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities	896:994	obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities	896:994	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	7	12	attach	presence	1400:1407	arg1	TPs					1451:1453	the TPs	1447:1453	the TPs	1447:1453	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	7	12	attach	presence	1400:1407	arg2	compounds					1421:1429	phenolic compounds	1412:1429	phenolic compounds	1412:1429	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	7	12	attach	presence	1400:1407	arg2	proteins					1435:1442	proteins	1435:1442	proteins	1435:1442	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	0	13	theme	Anti-Diabetic	109:121	arg1	Activities					123:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	7	14	theme	compounds	1421:1429	arg1	contributors					1473:1484	the main contributors	1464:1484	the main contributors to their bioactivities	1464:1507	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	7	14	theme	compounds	1421:1429	arg1	presence					1400:1407	the presence	1396:1407	the presence of phenolic compounds and proteins in the TPs	1396:1453	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	4	15	theme	TPs	758:760	arg1	compositions					689:700	The chemical compositions	676:700	The chemical compositions	676:700	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	4	15	theme	TPs	758:760	arg1	weight					713:718	molecular weight	703:718	molecular weight	703:718	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	4	15	theme	TPs	758:760	arg1	monosaccharides					739:753	compositional monosaccharides	725:753	compositional monosaccharides	725:753	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	4	16	theme	chemical	680:687	arg1	compositions					689:700	The chemical compositions	676:700	The chemical compositions	676:700	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	8	17	theme	crude	1601:1605	arg1	polysaccharides					1611:1625	crude tea polysaccharides	1601:1625	crude tea polysaccharides	1601:1625	The conclusion from this study can help understand the structural-function relationship of crude tea polysaccharides.					
34679697	3	18	theme	highest	635:641	arg1	%					672:672	6.38 ± 0.28%	661:672	6.38 ± 0.28%	661:672	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	18	theme	highest	635:641	arg1	yield					654:658	the highest extraction yield	631:658	the highest extraction yield (6.38 ± 0.28%)	631:673	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	8	19	from	study	1535:1539	arg1	conclusion					1514:1523	The conclusion	1510:1523	The conclusion from this study	1510:1539	The conclusion from this study can help understand the structural-function relationship of crude tea polysaccharides.					
34679697	7	20	from	TPs	1296:1298	arg1	phenolic					1263:1270	phenolic	1263:1270	phenolic	1263:1270	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	6	21	dep	phenolic	1071:1078	arg1	highest					1057:1063	highest	1057:1063	highest	1057:1063	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	7	22	from	presence	1400:1407	arg1	TPs					1451:1453	the TPs	1447:1453	the TPs	1447:1453	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	3	23	theme	extraction	643:652	arg1	%					672:672	6.38 ± 0.28%	661:672	6.38 ± 0.28%	661:672	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	23	theme	extraction	643:652	arg1	yield					654:658	the highest extraction yield	631:658	the highest extraction yield (6.38 ± 0.28%)	631:673	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	7	24	theme	structural-function	1190:1208	arg1	relationship					1210:1221	the structural-function relationship	1186:1221	the structural-function relationship	1186:1221	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	5	25	theme	protein-bound	832:844	arg1	heteropolysaccharides					851:871	protein-bound acid heteropolysaccharides	832:871	protein-bound acid heteropolysaccharides	832:871	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	2	26	theme	Tea	321:323	arg1	polysaccharides					325:339	Tea polysaccharides	321:339	Tea polysaccharides (TPs)	321:345	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	2	26	theme	Tea	321:323	arg1	TPs					342:344	TPs	342:344	TPs	342:344	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	0	27	theme	Structural	0:9	arg1	Characteristics					11:25	Structural Characteristics	0:25	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas	0:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	5	28	theme	acid	846:849	arg1	heteropolysaccharides					851:871	protein-bound acid heteropolysaccharides	832:871	protein-bound acid heteropolysaccharides	832:871	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	0	29	theme	Crude	30:34	arg1	Polysaccharides					36:50	Crude Polysaccharides	30:50	Crude Polysaccharides from 12 Selected Chinese Teas	30:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	1	30	theme	white	254:258	arg1	tea					260:262	white tea	254:262	white tea	254:262	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	30	theme	white	254:258	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	6	31	theme	anti-diabetic	1146:1158	arg1	activities					1160:1169	the best antioxidant and anti-diabetic activities	1121:1169	the best antioxidant and anti-diabetic activities	1121:1169	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	7	32	theme	antioxidant	1338:1348	arg1	activities					1368:1377	their antioxidant and anti-diabetic activities	1332:1377	their antioxidant and anti-diabetic activities	1332:1377	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	4	33	theme	molecular	703:711	arg1	weight					713:718	molecular weight	703:718	molecular weight	703:718	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	6	34	contain	contained	1043:1051	arg1	polysaccharides					1022:1036	Pu-erh tea polysaccharides	1011:1036	Pu-erh tea polysaccharides	1011:1036	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	6	34	contain	contained	1043:1051	arg2	phenolic					1071:1078	phenolic	1071:1078	phenolic	1071:1078	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	6	34	contain	contained	1043:1051	arg2	contents					1092:1099	the highest total phenolic and protein contents	1053:1099	contents	1092:1099	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	6	35	theme	Pu-erh	1011:1016	arg1	polysaccharides					1022:1036	Pu-erh tea polysaccharides	1011:1036	Pu-erh tea polysaccharides	1011:1036	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	5	36	dep	anti-diabetic	920:932	arg1	antiglycation					970:982	antiglycation	970:982	antiglycation	970:982	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	5	36	dep	anti-diabetic	920:932	arg1	inhibitory					955:964	inhibitory	955:964	inhibitory	955:964	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	3	37	theme	±	666:666	arg1	%					672:672	6.38 ± 0.28%	661:672	6.38 ± 0.28%	661:672	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	37	theme	±	666:666	arg1	yield					654:658	the highest extraction yield	631:658	the highest extraction yield (6.38 ± 0.28%)	631:673	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	38	theme	6.38	589:592	arg1	%					584:584	%	584:584	%	584:584	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	39	theme	extraction	543:552	arg1	yields					554:559	the extraction yields	539:559	the extraction yields of TPs	539:566	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	7	40	theme	map	1233:1235	arg1	analysis					1237:1244	the heat map analysis	1224:1244	the heat map analysis	1224:1244	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	0	41	theme	Polysaccharides	36:50	arg1	Activities					123:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	0	41	theme	Polysaccharides	36:50	arg1	Characteristics					11:25	Structural Characteristics	0:25	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas	0:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	1	42	theme	yellow	265:270	arg1	tea					272:274	yellow tea	265:274	yellow tea	265:274	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	42	theme	yellow	265:270	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	2	43	theme	structural	388:397	arg1	antioxidant					444:454	antioxidant	444:454	antioxidant	444:454	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	2	43	theme	structural	388:397	arg1	activities					474:483	anti-diabetic activities	460:483	anti-diabetic activities	460:483	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	2	43	theme	structural	388:397	arg1	properties					399:408	their structural properties	382:408	their structural properties	382:408	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	0	44	from	Teas	77:80	arg1	Activities					123:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Their Antioxidant and Anti-Diabetic Activities	87:132	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	0	44	from	Teas	77:80	arg1	Characteristics					11:25	Structural Characteristics	0:25	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas	0:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	0	44	from	Teas	77:80	arg1	Polysaccharides					36:50	Crude Polysaccharides	30:50	Crude Polysaccharides from 12 Selected Chinese Teas	30:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	2	45	theme	hot	367:369	arg1	water					371:375	hot water	367:375	hot water	367:375	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	1	46	from	categories	209:218	arg1	Camellia					178:185	Camellia	178:185	Camellia	178:185	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	46	from	categories	209:218	arg1	teas					172:175	Twelve representative edible Chinese teas	135:175	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	135:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	0	47	theme	Selected	60:67	arg1	Teas					77:80	12 Selected Chinese Teas	57:80	12 Selected Chinese Teas	57:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	2	48	theme	anti-diabetic	460:472	arg1	activities					474:483	anti-diabetic activities	460:483	anti-diabetic activities	460:483	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	2	48	theme	anti-diabetic	460:472	arg1	properties					399:408	their structural properties	382:408	their structural properties	382:408	Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated.					
34679697	5	49	theme	anti-diabetic	920:932	arg1	activities					985:994	obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities	896:994	obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities	896:994	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	1	50	dep	Camellia	178:185	arg1	L.					196:197	Camellia sinensis L.	178:197	Camellia sinensis L.	178:197	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	7	51	theme	proteins	1435:1442	arg1	contributors					1473:1484	the main contributors	1464:1484	the main contributors to their bioactivities	1464:1507	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	7	51	theme	proteins	1435:1442	arg1	presence					1400:1407	the presence	1396:1407	the presence of phenolic compounds and proteins in the TPs	1396:1453	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	8	52	theme	tea	1607:1609	arg1	polysaccharides					1611:1625	crude tea polysaccharides	1601:1625	crude tea polysaccharides	1601:1625	The conclusion from this study can help understand the structural-function relationship of crude tea polysaccharides.					
34679697	4	53	theme	tea	797:799	arg1	categories					783:792	the six categories	775:792	the six categories of tea	775:799	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	4	54	theme	compositional	725:737	arg1	monosaccharides					739:753	compositional monosaccharides	725:753	compositional monosaccharides	725:753	The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea.					
34679697	3	55	theme	1.81	580:583	arg1	%					584:584	%	584:584	%	584:584	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	56	theme	TPs	564:566	arg1	yields					554:559	the extraction yields	539:559	the extraction yields of TPs	539:566	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	3	57	theme	Pu-erh	600:605	arg1	polysaccharides					611:625	Pu-erh tea polysaccharides	600:625	Pu-erh tea polysaccharides	600:625	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	1	58	dep	categories	209:218	arg1	tea					272:274	yellow tea	265:274	yellow tea	265:274	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	58	dep	categories	209:218	arg1	tea					226:228	dark tea	221:228	dark tea	221:228	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	58	dep	categories	209:218	arg1	tea					260:262	white tea	254:262	white tea	254:262	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	58	dep	categories	209:218	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	58	dep	categories	209:218	arg1	tea					287:289	green tea	281:289	green tea	281:289	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	58	dep	categories	209:218	arg1	tea					237:239	black tea	231:239	black tea	231:239	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	58	dep	categories	209:218	arg1	tea					249:251	oolong tea	242:251	oolong tea	242:251	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	8	59	theme	polysaccharides	1611:1625	arg1	relationship					1585:1596	the structural-function relationship	1561:1596	the structural-function relationship of crude tea polysaccharides	1561:1625	The conclusion from this study can help understand the structural-function relationship of crude tea polysaccharides.					
34679697	1	60	theme	dark	221:224	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	60	theme	dark	221:224	arg1	tea					226:228	dark tea	221:228	dark tea	221:228	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	7	61	theme	heat	1228:1231	arg1	analysis					1237:1244	the heat map analysis	1224:1244	the heat map analysis	1224:1244	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	5	62	theme	antioxidant	904:914	arg1	activities					985:994	obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities	896:994	obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities	896:994	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	1	63	theme	representative	142:155	arg1	Camellia					178:185	Camellia	178:185	Camellia	178:185	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	63	theme	representative	142:155	arg1	teas					172:175	Twelve representative edible Chinese teas	135:175	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	135:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	3	64	dep	%	584:584	arg1	to					586:587	to	586:587	to	586:587	Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%).					
34679697	7	65	from	phenolic	1263:1270	arg1	TPs					1296:1298	TPs	1296:1298	TPs	1296:1298	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	1	66	theme	edible	157:162	arg1	Camellia					178:185	Camellia	178:185	Camellia	178:185	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	66	theme	edible	157:162	arg1	teas					172:175	Twelve representative edible Chinese teas	135:175	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	135:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	7	67	theme	protein	1276:1282	arg1	contents					1284:1291	total phenolic and protein contents	1257:1291	contents	1284:1291	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	6	68	theme	protein	1084:1090	arg1	contents					1092:1099	the highest total phenolic and protein contents	1053:1099	contents	1092:1099	Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities.					
34679697	0	69	theme	Chinese	69:75	arg1	Teas					77:80	12 Selected Chinese Teas	57:80	12 Selected Chinese Teas	57:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	1	70	theme	green	281:285	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	70	theme	green	281:285	arg1	tea					287:289	green tea	281:289	green tea	281:289	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	0	71	from	Activities	123:132	arg1	Teas					77:80	12 Selected Chinese Teas	57:80	12 Selected Chinese Teas	57:80	Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities.					
34679697	7	72	theme	main	1468:1471	arg1	contributors					1473:1484	the main contributors	1464:1484	the main contributors to their bioactivities	1464:1507	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	7	72	theme	main	1468:1471	arg1	presence					1400:1407	the presence	1396:1407	the presence of phenolic compounds and proteins in the TPs	1396:1453	Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities.					
34679697	1	73	theme	Chinese	164:170	arg1	Camellia					178:185	Camellia	178:185	Camellia	178:185	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	73	theme	Chinese	164:170	arg1	teas					172:175	Twelve representative edible Chinese teas	135:175	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	135:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	5	74	dep	inhibitory	955:964	arg1	e.g.					935:938	e.g.	935:938	e.g.	935:938	It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities.					
34679697	1	75	theme	black	231:235	arg1	categories					209:218	six categories	205:218	six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea)	205:290	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
34679697	1	75	theme	black	231:235	arg1	tea					237:239	black tea	231:239	black tea	231:239	Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study.					
33471208	12	0	theme	restorations	1727:1738	arg1	survival					1711:1718	the survival	1707:1718	the survival of the restorations after 1 year	1707:1751	The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year.					
33471208	6	1	theme	photographic	821:832	arg1	criteria					886:893	modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria	734:893	modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria	734:893	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	10	2	theme	adaptation	1358:1367	arg1	%					1261:1261	91%	1259:1261	91% of the NanoChi restorations	1259:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	2	theme	adaptation	1358:1367	arg1	criteria					1390:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	2	theme	adaptation	1358:1367	arg1	restorations					1278:1289	the NanoChi restorations	1266:1289	the NanoChi restorations	1266:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	6	3	theme	modified	734:741	arg1	criteria					886:893	modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria	734:893	modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria	734:893	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	3	4	theme	2.5	516:518	arg1	%					519:519	%	519:519	%	519:519	The teeth were randomly assigned according to dentin treatment: with 2.5% NanoChi and without with chitosan (control).					
33471208	8	5	theme	significant	1049:1059	arg1	difference					1061:1070	No significant difference	1046:1070	No significant difference	1046:1070	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	1	6	theme	pre-treatment	178:190	arg1	influence					161:169	the influence	157:169	the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment	157:306	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	10	7	theme	form	1385:1388	arg1	%					1261:1261	91%	1259:1261	91% of the NanoChi restorations	1259:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	7	theme	form	1385:1388	arg1	criteria					1390:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	7	theme	form	1385:1388	arg1	restorations					1278:1289	the NanoChi restorations	1266:1289	the NanoChi restorations	1266:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	1	8	theme	2.5	197:199	arg1	%					200:200	%	200:200	%	200:200	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	11	9	theme	color	1542:1546	arg1	criteria					1575:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	11	9	theme	color	1542:1546	arg1	Alpha					1599:1603	Alpha	1599:1603	Alpha	1599:1603	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	5	10	theme	Bond	659:662	arg1	3 M					675:677	Single Bond Universal (3 M)	652:678	Single Bond Universal (3 M)	652:678	The teeth were restored with Single Bond Universal (3 M) and Charisma resin (Kulzer).					
33471208	13	11	theme	resin	1832:1836	arg1	restorations					1838:1849	resin restorations	1832:1849	resin restorations	1832:1849	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	1	12	theme	nanoparticulate	202:216	arg1	NanoChi					233:239	2.5% NanoChi	228:239	2.5% NanoChi	228:239	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	1	12	theme	nanoparticulate	202:216	arg1	chitosan					218:225	nanoparticulate chitosan	202:225	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	8	13	theme	survival	1089:1096	arg1	rates					1098:1102	the survival rates	1085:1102	the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment	1085:1165	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	6	14	theme	anatomical	869:878	arg1	form					880:883	anatomical form	869:883	anatomical form	869:883	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	15	dep	USPHS	743:747	arg1	retention					750:758	retention	750:758	retention	750:758	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	15	dep	USPHS	743:747	arg1	caries					771:776	secondary caries	761:776	secondary caries	761:776	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	15	dep	USPHS	743:747	arg1	adaptation					788:797	marginal adaptation	779:797	marginal adaptation	779:797	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	15	dep	USPHS	743:747	arg1	sensitivity					804:814	sensitivity	804:814	sensitivity	804:814	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	7	16	theme	Kaplan-Meier	953:964	arg1	estimates					966:974	Kaplan-Meier estimates	953:974	Kaplan-Meier estimates	953:974	Population demographics, Kaplan-Meier estimates and log-rank test (Mantel-Cox) were calculated for 1 year (α = 0.05).					
33471208	11	17	theme	pigmentation	1562:1573	arg1	criteria					1575:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	11	17	theme	pigmentation	1562:1573	arg1	Alpha					1599:1603	Alpha	1599:1603	Alpha	1599:1603	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	6	18	theme	marginal	842:849	arg1	pigmentation					851:862	marginal pigmentation	842:862	marginal pigmentation	842:862	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	0	19	theme	eroded	65:70	arg1	dentin					72:77	eroded dentin	65:77	eroded dentin	65:77	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	11	20	theme	sensitivity	1529:1539	arg1	criteria					1575:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	11	20	theme	sensitivity	1529:1539	arg1	Alpha					1599:1603	Alpha	1599:1603	Alpha	1599:1603	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	12	21	theme	eroded	1657:1662	arg1	dentin					1664:1669	eroded dentin	1657:1669	eroded dentin	1657:1669	The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year.					
33471208	1	22	from	influence	161:169	arg1	dentin					261:266	eroded dentin	254:266	eroded dentin before the restorative dental treatment	254:306	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	0	23	from	Influence	0:8	arg1	biomodification					46:60	the biomodification	42:60	the biomodification of eroded dentin	42:77	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	11	24	theme	caries	1521:1526	arg1	criteria					1575:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	11	24	theme	caries	1521:1526	arg1	Alpha					1599:1603	Alpha	1599:1603	Alpha	1599:1603	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	10	25	theme	restorations	1278:1289	arg1	criteria					1390:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	25	theme	restorations	1278:1289	arg1	%					1261:1261	91%	1259:1261	91% of the NanoChi restorations	1259:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	25	theme	restorations	1278:1289	arg1	restorations					1278:1289	the NanoChi restorations	1266:1289	the NanoChi restorations	1266:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	26	theme	restorations	1425:1436	arg1	restorations					1425:1436	the control restorations	1413:1436	the control restorations (without NanoChi)	1413:1454	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	26	theme	restorations	1425:1436	arg1	%					1408:1408	86%	1406:1408	86% of the control restorations (without NanoChi)	1406:1454	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	0	27	theme	longitudinal	106:117	arg1	analysis					119:126	clinical and photographic longitudinal analysis	80:126	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.	0:143	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	13	28	theme	%	1776:1776	arg1	NanoChi					1778:1784	2.5% NanoChi	1773:1784	2.5% NanoChi	1773:1784	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	2	29	from	teeth	440:444	arg1	located					414:420	located	414:420	located	414:420	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	10	30	theme	Alpha	1306:1310	arg1	%					1261:1261	91%	1259:1261	91% of the NanoChi restorations	1259:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	30	theme	Alpha	1306:1310	arg1	criteria					1390:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	30	theme	Alpha	1306:1310	arg1	restorations					1278:1289	the NanoChi restorations	1266:1289	the NanoChi restorations	1266:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	2	31	dep	patients	336:343	arg1	age					346:348	age	346:348	age	346:348	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	2	32	theme	medium	391:396	arg1	lesions					406:412	shallow or medium erosion lesions	380:412	lesions	406:412	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	0	33	theme	nanoparticulated	13:28	arg1	chitosan					30:37	nanoparticulated chitosan	13:37	nanoparticulated chitosan	13:37	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	1	34	theme	restorative	279:289	arg1	treatment					298:306	the restorative dental treatment	275:306	the restorative dental treatment	275:306	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	2	35	theme	shallow	380:386	arg1	lesions					406:412	shallow or medium erosion lesions	380:412	lesions	406:412	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	13	36	theme	pre-treatment	1888:1900	arg1	it					1868:1869	it	1868:1869	it	1868:1869	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	13	36	theme	pre-treatment	1888:1900	arg1	solution					1902:1909	a pre-treatment solution	1886:1909	a pre-treatment solution	1886:1909	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	2	37	with	patients	336:343	arg1	lesions					406:412	shallow or medium erosion lesions	380:412	lesions	406:412	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	2	38	theme	homologous	429:438	arg1	teeth					440:444	two homologous teeth	425:444	two homologous teeth	425:444	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	8	39	located	found	1076:1080	arg2	difference					1061:1070	No significant difference	1046:1070	No significant difference	1046:1070	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	8	39	located	found	1076:1080	arg1	rates					1098:1102	the survival rates	1085:1102	the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment	1085:1165	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	6	40	theme	marginal	779:786	arg1	adaptation					788:797	marginal adaptation	779:797	marginal adaptation	779:797	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	1	41	with	pre-treatment	178:190	arg1	solution					242:249	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	0	42	dep	Influence	0:8	arg1	analysis					119:126	clinical and photographic longitudinal analysis	80:126	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.	0:143	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	12	43	with	biomodification	1638:1652	arg1	NanoChi					1681:1687	2.5% NanoChi	1676:1687	2.5% NanoChi	1676:1687	The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year.					
33471208	6	44	dep	photographic	821:832	arg1	form					880:883	anatomical form	869:883	anatomical form	869:883	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	44	dep	photographic	821:832	arg1	color					835:839	color	835:839	color	835:839	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	44	dep	photographic	821:832	arg1	pigmentation					851:862	marginal pigmentation	842:862	marginal pigmentation	842:862	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	6	45	theme	USPHS	743:747	arg1	criteria					886:893	modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria	734:893	modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria	734:893	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	1	46	theme	eroded	254:259	arg1	dentin					261:266	eroded dentin	254:266	eroded dentin before the restorative dental treatment	254:306	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	10	47	theme	marginal	1349:1356	arg1	adaptation					1358:1367	marginal adaptation	1349:1367	marginal adaptation	1349:1367	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	4	48	theme	acid	609:612	arg1	etching					614:620	acid etching	609:620	acid etching	609:620	The NanoChi were applied immediately after acid etching.					
33471208	7	49	theme	log-rank	980:987	arg1	Mantel-Cox					995:1004	Mantel-Cox	995:1004	Mantel-Cox	995:1004	Population demographics, Kaplan-Meier estimates and log-rank test (Mantel-Cox) were calculated for 1 year (α = 0.05).					
33471208	7	49	theme	log-rank	980:987	arg1	test					989:992	log-rank test	980:992	log-rank test (Mantel-Cox)	980:1005	Population demographics, Kaplan-Meier estimates and log-rank test (Mantel-Cox) were calculated for 1 year (α = 0.05).					
33471208	10	50	theme	anatomical	1374:1383	arg1	form					1385:1388	anatomical form	1374:1388	anatomical form	1374:1388	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	11	51	theme	marginal	1553:1560	arg1	pigmentation					1562:1573	marginal pigmentation	1553:1573	marginal pigmentation	1553:1573	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	0	52	theme	restorations	131:142	arg1	analysis					119:126	clinical and photographic longitudinal analysis	80:126	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.	0:143	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	3	53	theme	%	519:519	arg1	NanoChi					521:527	2.5% NanoChi	516:527	2.5% NanoChi	516:527	The teeth were randomly assigned according to dentin treatment: with 2.5% NanoChi and without with chitosan (control).					
33471208	1	54	theme	%	200:200	arg1	solution					242:249	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	5	55	theme	Universal	664:672	arg1	3 M					675:677	Single Bond Universal (3 M)	652:678	Single Bond Universal (3 M)	652:678	The teeth were restored with Single Bond Universal (3 M) and Charisma resin (Kulzer).					
33471208	13	56	theme	restorations	1838:1849	arg1	failures					1820:1827	failures	1820:1827	failures of resin restorations	1820:1849	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	1	57	theme	chitosan	218:225	arg1	solution					242:249	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	12	58	theme	%	1679:1679	arg1	NanoChi					1681:1687	2.5% NanoChi	1676:1687	2.5% NanoChi	1676:1687	The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year.					
33471208	7	59	theme	Population	928:937	arg1	demographics					939:950	Population demographics	928:950	Population demographics	928:950	Population demographics, Kaplan-Meier estimates and log-rank test (Mantel-Cox) were calculated for 1 year (α = 0.05).					
33471208	2	60	from	located	414:420	arg1	teeth					440:444	two homologous teeth	425:444	two homologous teeth	425:444	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	5	61	theme	Single	652:657	arg1	3 M					675:677	Single Bond Universal (3 M)	652:678	Single Bond Universal (3 M)	652:678	The teeth were restored with Single Bond Universal (3 M) and Charisma resin (Kulzer).					
33471208	11	62	theme	Secondary	1511:1519	arg1	criteria					1575:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria	1511:1582	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	11	62	theme	Secondary	1511:1519	arg1	Alpha					1599:1603	Alpha	1599:1603	Alpha	1599:1603	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	0	63	theme	dentin	72:77	arg1	biomodification					46:60	the biomodification	42:60	the biomodification of eroded dentin	42:77	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	8	64	from	days	1135:1138	arg1	p > 0.05					1120:1127	p > 0.05	1120:1127	p > 0.05	1120:1127	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	8	64	from	days	1135:1138	arg1	groups					1112:1117	groups	1112:1117	groups (p > 0.05) at 7 days	1112:1138	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	8	64	from	days	1135:1138	arg1	year					1146:1149	1 year	1144:1149	1 year after treatment	1144:1165	No significant difference was found in the survival rates between groups (p > 0.05) at 7 days and 1 year after treatment.					
33471208	0	65	theme	clinical	80:87	arg1	analysis					119:126	clinical and photographic longitudinal analysis	80:126	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.	0:143	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	13	66	theme	eroded	1789:1794	arg1	dentin					1796:1801	eroded dentin	1789:1801	eroded dentin	1789:1801	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	12	67	theme	dentin	1664:1669	arg1	biomodification					1638:1652	The biomodification	1634:1652	The biomodification of eroded dentin with 2.5% NanoChi	1634:1687	The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year.					
33471208	0	68	theme	photographic	93:104	arg1	analysis					119:126	clinical and photographic longitudinal analysis	80:126	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.	0:143	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	12	69	theme	2.5	1676:1678	arg1	%					1679:1679	%	1679:1679	%	1679:1679	The biomodification of eroded dentin with 2.5% NanoChi did not influence the survival of the restorations after 1 year.					
33471208	13	70	theme	2.5	1773:1775	arg1	%					1776:1776	%	1776:1776	%	1776:1776	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	13	71	from	application	1758:1768	arg1	dentin					1796:1801	eroded dentin	1789:1801	eroded dentin	1789:1801	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	10	72	theme	NanoChi	1270:1276	arg1	restorations					1278:1289	the NanoChi restorations	1266:1289	the NanoChi restorations	1266:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	73	theme	control	1417:1423	arg1	restorations					1425:1436	the control restorations	1413:1436	the control restorations (without NanoChi)	1413:1454	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	13	74	theme	NanoChi	1778:1784	arg1	application					1758:1768	The application	1754:1768	The application of 2.5% NanoChi on eroded dentin	1754:1801	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	2	75	theme	located	414:420	arg1	lesions					406:412	shallow or medium erosion lesions	380:412	lesions	406:412	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	0	76	theme	chitosan	30:37	arg1	Influence					0:8	Influence	0:8	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.	0:143	Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations.					
33471208	2	77	theme	erosion	398:404	arg1	lesions					406:412	shallow or medium erosion lesions	380:412	lesions	406:412	The sample consisted of 22 patients (age between 33 and 52 years) with shallow or medium erosion lesions located in two homologous teeth.					
33471208	1	78	theme	dental	291:296	arg1	treatment					298:306	the restorative dental treatment	275:306	the restorative dental treatment	275:306	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	5	79	theme	Charisma	684:691	arg1	Kulzer					700:705	Kulzer	700:705	Kulzer	700:705	The teeth were restored with Single Bond Universal (3 M) and Charisma resin (Kulzer).					
33471208	5	79	theme	Charisma	684:691	arg1	resin					693:697	Charisma resin	684:697	Charisma resin (Kulzer)	684:706	The teeth were restored with Single Bond Universal (3 M) and Charisma resin (Kulzer).					
33471208	9	80	theme	restorations	1194:1205	arg1	%					1185:1185	100%	1182:1185	100% of the restorations	1182:1205	After 7 days, 100% of the restorations were scored as Alpha on all criteria.					
33471208	9	80	theme	restorations	1194:1205	arg1	restorations					1194:1205	the restorations	1190:1205	the restorations	1190:1205	After 7 days, 100% of the restorations were scored as Alpha on all criteria.					
33471208	9	80	theme	restorations	1194:1205	arg1	Alpha					1222:1226	Alpha	1222:1226	Alpha	1222:1226	After 7 days, 100% of the restorations were scored as Alpha on all criteria.					
33471208	10	81	theme	%	1317:1317	arg1	%					1261:1261	91%	1259:1261	91% of the NanoChi restorations	1259:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	81	theme	%	1317:1317	arg1	criteria					1390:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria	1306:1397	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	81	theme	%	1317:1317	arg1	restorations					1278:1289	the NanoChi restorations	1266:1289	the NanoChi restorations	1266:1289	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	10	82	theme	Alpha	1469:1473	arg1	score					1475:1479	the Alpha score	1465:1479	the Alpha score	1465:1479	After 1 year, 91% of the NanoChi restorations were scored as Alpha and 9% as Charlie for the retention, marginal adaptation, and anatomical form criteria, while 86% of the control restorations (without NanoChi) received the Alpha score and 14% received the Charlie.					
33471208	13	83	used	used	1878:1881	arg2	solution					1902:1909	a pre-treatment solution	1886:1909	a pre-treatment solution	1886:1909	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	13	83	used	used	1878:1881	arg2	it					1868:1869	it	1868:1869	it	1868:1869	The application of 2.5% NanoChi on eroded dentin did not increase failures of resin restorations after 1 year and it can be used as a pre-treatment solution.					
33471208	6	84	theme	secondary	761:769	arg1	caries					771:776	secondary caries	761:776	secondary caries	761:776	Analyzes were done using modified USPHS (retention, secondary caries, marginal adaptation, and sensitivity) and photographic (color, marginal pigmentation, and anatomical form) criteria at 7 days (baseline) and 1 year.					
33471208	3	85	theme	dentin	493:498	arg1	treatment					500:508	dentin treatment	493:508	dentin treatment	493:508	The teeth were randomly assigned according to dentin treatment: with 2.5% NanoChi and without with chitosan (control).					
33471208	1	86	theme	%	231:231	arg1	NanoChi					233:239	2.5% NanoChi	228:239	2.5% NanoChi	228:239	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	1	86	theme	%	231:231	arg1	chitosan					218:225	nanoparticulate chitosan	202:225	2.5% nanoparticulate chitosan (2.5% NanoChi) solution	197:249	To evaluate the influence of the pre-treatment with 2.5% nanoparticulate chitosan (2.5% NanoChi) solution on eroded dentin before the restorative dental treatment.					
33471208	11	87	theme	restorations	1620:1631	arg1	%					1611:1611	100%	1608:1611	100% of the restorations	1608:1631	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
33471208	11	87	theme	restorations	1620:1631	arg1	restorations					1620:1631	the restorations	1616:1631	the restorations	1616:1631	Secondary caries, sensitivity, color, and marginal pigmentation criteria were scored as Alpha in 100% of the restorations.					
32374794	0	0	theme	synergistic	83:93	arg1	effects					95:101	the synergistic effects	79:101	the synergistic effects of multiple dynamic bonds	79:127	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	4	1	theme	mechanical	620:629	arg1	properties					631:640	tunable mechanical properties	612:640	tunable mechanical properties	612:640	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	1	2	theme	gelatin	156:162	arg1	hydrogel					144:151	The composite hydrogel	130:151	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA)	130:203	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	8	3	dep	cytotoxicity	1330:1341	arg1	the					1315:1317	the	1315:1317	the	1315:1317	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	8	3	dep	cytotoxicity	1330:1341	arg1	absence					1319:1325	absence	1319:1325	absence	1319:1325	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	7	4	theme	great	1150:1154	arg1	conductivity					1156:1167	great conductivity	1150:1167	great conductivity	1150:1167	Interestingly, the hydrogels presented great conductivity due to the existence of free ions, varying with the deformation of hydrogels, and showed great potential in promoting osteogenesis.					
32374794	6	5	with	endowed	1042:1048	arg1	performance					1098:1108	good self-healing performance	1080:1108	good self-healing performance	1080:1108	These multiple dynamic bonds also endowed the Gel-OSA-PVA hydrogel with good self-healing performance.					
32374794	4	6	theme	tunable	612:618	arg1	properties					631:640	tunable mechanical properties	612:640	tunable mechanical properties	612:640	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	8	7	theme	biomedical	1435:1444	arg1	field					1446:1450	the biomedical field	1431:1450	the biomedical field	1431:1450	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	1	8	theme	biomedical	222:231	arg1	fields					233:238	biomedical fields	222:238	biomedical fields due to its good biocompatibility	222:271	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	8	9	from	applications	1415:1426	arg1	field					1446:1450	the biomedical field	1431:1450	the biomedical field	1431:1450	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	4	10	contain	possessed	602:610	arg1	hydrogel					593:600	The hydrogel	589:600	The hydrogel	589:600	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	4	10	contain	possessed	602:610	arg2	properties					631:640	tunable mechanical properties	612:640	tunable mechanical properties	612:640	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	8	11	theme	antibacterial	1347:1359	arg1	properties					1361:1370	antibacterial properties	1347:1370	antibacterial properties	1347:1370	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	4	12	theme	oxidized	689:696	arg1	degrees					698:704	the oxidized degrees	685:704	the oxidized degrees of OSA	685:711	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	5	13	theme	group	858:862	arg1	content					864:870	its aldehyde group content	845:870	its aldehyde group content	845:870	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	5	14	theme	length	1000:1005	arg1	change					967:972	its change	963:972	its change of molecular weight/chain length	963:1005	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	2	15	theme	weak	316:319	arg1	properties					332:341	weak mechanical properties	316:341	weak mechanical properties	316:341	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	1	16	theme	due	240:242	arg1	fields					233:238	biomedical fields	222:238	biomedical fields due to its good biocompatibility	222:271	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	0	17	theme	dynamic	115:121	arg1	bonds					123:127	multiple dynamic bonds	106:127	multiple dynamic bonds	106:127	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	3	18	theme	vinyl	434:438	arg1	poly					429:432	poly	429:432	poly(vinyl alcohol) (PVA)	429:453	Herein, poly(vinyl alcohol) (PVA) is mixed with Gel and OSA to construct a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity.					
32374794	3	18	theme	vinyl	434:438	arg1	alcohol					440:446	vinyl alcohol	434:446	vinyl alcohol	434:446	Herein, poly(vinyl alcohol) (PVA) is mixed with Gel and OSA to construct a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity.					
32374794	1	19	theme	alginate	190:197	arg1	hydrogel					144:151	The composite hydrogel	130:151	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA)	130:203	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	6	20	theme	self-healing	1085:1096	arg1	performance					1098:1108	good self-healing performance	1080:1108	good self-healing performance	1080:1108	These multiple dynamic bonds also endowed the Gel-OSA-PVA hydrogel with good self-healing performance.					
32374794	5	21	theme	weight/chain	987:998	arg1	length					1000:1005	molecular weight/chain length	977:1005	molecular weight/chain length	977:1005	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	0	22	theme	multiple	106:113	arg1	bonds					123:127	multiple dynamic bonds	106:127	multiple dynamic bonds	106:127	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	2	23	theme	typical	287:293	arg1	preferred					351:359	preferred	351:359	preferred	351:359	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	2	23	theme	typical	287:293	arg1	utility					295:301	the typical utility	283:301	the typical utility of borax and weak mechanical properties	283:341	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	2	24	theme	supporting	400:409	arg1	scaffold					411:418	a supporting scaffold	398:418	a supporting scaffold	398:418	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	4	25	theme	hydrogen	770:777	arg1	bonds					779:783	multiple hydrogen bonds	761:783	multiple hydrogen bonds	761:783	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	7	26	theme	ions	1198:1201	arg1	existence					1180:1188	the existence	1176:1188	the existence of free ions, varying with the deformation of hydrogels,	1176:1245	Interestingly, the hydrogels presented great conductivity due to the existence of free ions, varying with the deformation of hydrogels, and showed great potential in promoting osteogenesis.					
32374794	7	27	theme	hydrogels	1236:1244	arg1	deformation					1221:1231	the deformation	1217:1231	the deformation of hydrogels	1217:1244	Interestingly, the hydrogels presented great conductivity due to the existence of free ions, varying with the deformation of hydrogels, and showed great potential in promoting osteogenesis.					
32374794	0	28	theme	Ternary	0:6	arg1	hydrogels					8:16	Ternary hydrogels	0:16	Ternary hydrogels with tunable mechanical and self-healing properties	0:68	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	4	29	theme	multiple	761:768	arg1	bonds					779:783	multiple hydrogen bonds	761:783	multiple hydrogen bonds	761:783	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	5	30	theme	hydrogels	941:949	arg1	modulus					930:936	the compressive modulus	914:936	the compressive modulus of hydrogels compared to its change of molecular weight/chain length	914:1005	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	1	31	theme	oxidized	174:181	arg1	alginate					190:197	oxidized sodium alginate	174:197	oxidized sodium alginate (OSA)	174:203	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	1	31	theme	oxidized	174:181	arg1	OSA					200:202	OSA	200:202	OSA	200:202	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	0	32	theme	tunable	23:29	arg1	properties					59:68	tunable mechanical and self-healing properties	23:68	tunable mechanical and self-healing properties	23:68	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	4	33	theme	dynamic	737:743	arg1	bonds					751:755	dynamic imine bonds	737:755	dynamic imine bonds	737:755	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	5	34	theme	aldehyde	849:856	arg1	content					864:870	its aldehyde group content	845:870	its aldehyde group content	845:870	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	1	35	theme	sodium	183:188	arg1	alginate					190:197	oxidized sodium alginate	174:197	oxidized sodium alginate (OSA)	174:203	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	1	35	theme	sodium	183:188	arg1	OSA					200:202	OSA	200:202	OSA	200:202	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	4	36	dep	bonds	751:755	arg1	the					733:735	the	733:735	the	733:735	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	1	37	theme	good	251:254	arg1	biocompatibility					256:271	its good biocompatibility	247:271	its good biocompatibility	247:271	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	0	38	theme	bonds	123:127	arg1	effects					95:101	the synergistic effects	79:101	the synergistic effects of multiple dynamic bonds	79:127	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	4	39	theme	PVA	677:679	arg1	concentrations					659:672	the concentrations	655:672	the concentrations of PVA	655:679	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	4	39	theme	PVA	677:679	arg1	degrees					698:704	the oxidized degrees	685:704	the oxidized degrees of OSA	685:711	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	2	40	theme	cell	375:378	arg1	environment					380:390	the cell environment	371:390	the cell environment	371:390	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	3	41	theme	hydrogel	504:511	arg1	Gel-OSA-PVA					513:523	a ternary hydrogel Gel-OSA-PVA	494:523	a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity	494:586	Herein, poly(vinyl alcohol) (PVA) is mixed with Gel and OSA to construct a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity.					
32374794	0	42	theme	mechanical	31:40	arg1	properties					59:68	tunable mechanical and self-healing properties	23:68	tunable mechanical and self-healing properties	23:68	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	5	43	theme	molecular	977:985	arg1	length					1000:1005	molecular weight/chain length	977:1005	molecular weight/chain length	977:1005	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	5	44	theme	compressive	918:928	arg1	modulus					930:936	the compressive modulus	914:936	the compressive modulus of hydrogels compared to its change of molecular weight/chain length	914:1005	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	3	45	theme	mechanical	546:555	arg1	properties					557:566	the mechanical properties	542:566	the mechanical properties	542:566	Herein, poly(vinyl alcohol) (PVA) is mixed with Gel and OSA to construct a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity.					
32374794	4	46	theme	imine	745:749	arg1	bonds					751:755	dynamic imine bonds	737:755	dynamic imine bonds	737:755	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	6	47	theme	multiple	1014:1021	arg1	bonds					1031:1035	These multiple dynamic bonds	1008:1035	These multiple dynamic bonds	1008:1035	These multiple dynamic bonds also endowed the Gel-OSA-PVA hydrogel with good self-healing performance.					
32374794	2	48	theme	borax	306:310	arg1	preferred					351:359	preferred	351:359	preferred	351:359	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	2	48	theme	borax	306:310	arg1	utility					295:301	the typical utility	283:301	the typical utility of borax and weak mechanical properties	283:341	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	0	49	theme	self-healing	46:57	arg1	properties					59:68	tunable mechanical and self-healing properties	23:68	tunable mechanical and self-healing properties	23:68	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	1	50	used	used	214:217	arg2	hydrogel					144:151	The composite hydrogel	130:151	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA)	130:203	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	3	51	theme	ternary	496:502	arg1	Gel-OSA-PVA					513:523	a ternary hydrogel Gel-OSA-PVA	494:523	a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity	494:586	Herein, poly(vinyl alcohol) (PVA) is mixed with Gel and OSA to construct a ternary hydrogel Gel-OSA-PVA so as to improve the mechanical properties and reduce toxicity.					
32374794	0	52	with	hydrogels	8:16	arg1	properties					59:68	tunable mechanical and self-healing properties	23:68	tunable mechanical and self-healing properties	23:68	Ternary hydrogels with tunable mechanical and self-healing properties based on the synergistic effects of multiple dynamic bonds.					
32374794	5	53	theme	effects	829:835	arg1	terms					816:820	terms	816:820	terms of the effects of OSA	816:842	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	8	54	theme	potential	1391:1399	arg1	candidate					1401:1409	a potential candidate	1389:1409	a potential candidate for applications in the biomedical field	1389:1450	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	8	54	theme	potential	1391:1399	arg1	hydrogel					1377:1384	the hydrogel	1373:1384	the hydrogel	1373:1384	Together with the absence of cytotoxicity and antibacterial properties, the hydrogel is a potential candidate for applications in the biomedical field.					
32374794	5	55	theme	important	886:894	arg1	role					896:899	a more important role	879:899	a more important role	879:899	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	2	56	from	preferred	351:359	arg1	environment					380:390	the cell environment	371:390	the cell environment	371:390	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	7	57	theme	free	1193:1196	arg1	ions					1198:1201	free ions	1193:1201	free ions	1193:1201	Interestingly, the hydrogels presented great conductivity due to the existence of free ions, varying with the deformation of hydrogels, and showed great potential in promoting osteogenesis.					
32374794	6	58	theme	Gel-OSA-PVA	1054:1064	arg1	hydrogel					1066:1073	the Gel-OSA-PVA hydrogel	1050:1073	the Gel-OSA-PVA hydrogel	1050:1073	These multiple dynamic bonds also endowed the Gel-OSA-PVA hydrogel with good self-healing performance.					
32374794	6	59	theme	good	1080:1083	arg1	performance					1098:1108	good self-healing performance	1080:1108	good self-healing performance	1080:1108	These multiple dynamic bonds also endowed the Gel-OSA-PVA hydrogel with good self-healing performance.					
32374794	4	60	theme	OSA	709:711	arg1	concentrations					659:672	the concentrations	655:672	the concentrations of PVA	655:679	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	4	60	theme	OSA	709:711	arg1	degrees					698:704	the oxidized degrees	685:704	the oxidized degrees of OSA	685:711	The hydrogel possessed tunable mechanical properties by adjusting the concentrations of PVA and the oxidized degrees of OSA, related closely to the dynamic imine bonds and multiple hydrogen bonds among these macromolecules.					
32374794	1	61	theme	composite	134:142	arg1	hydrogel					144:151	The composite hydrogel	130:151	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA)	130:203	The composite hydrogel of gelatin (Gel) and oxidized sodium alginate (OSA) is often used in biomedical fields due to its good biocompatibility.					
32374794	5	62	theme	OSA	840:842	arg1	effects					829:835	the effects	825:835	the effects of OSA	825:842	In terms of the effects of OSA, its aldehyde group content played a more important role in enhancing the compressive modulus of hydrogels compared to its change of molecular weight/chain length.					
32374794	7	63	theme	great	1258:1262	arg1	potential					1264:1272	great potential	1258:1272	great potential	1258:1272	Interestingly, the hydrogels presented great conductivity due to the existence of free ions, varying with the deformation of hydrogels, and showed great potential in promoting osteogenesis.					
32374794	2	64	theme	properties	332:341	arg1	preferred					351:359	preferred	351:359	preferred	351:359	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	2	64	theme	properties	332:341	arg1	utility					295:301	the typical utility	283:301	the typical utility of borax and weak mechanical properties	283:341	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	6	65	theme	dynamic	1023:1029	arg1	bonds					1031:1035	These multiple dynamic bonds	1008:1035	These multiple dynamic bonds	1008:1035	These multiple dynamic bonds also endowed the Gel-OSA-PVA hydrogel with good self-healing performance.					
32374794	2	66	from	environment	380:390	arg1	preferred					351:359	preferred	351:359	preferred	351:359	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	2	66	from	environment	380:390	arg1	utility					295:301	the typical utility	283:301	the typical utility of borax and weak mechanical properties	283:341	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
32374794	2	67	theme	mechanical	321:330	arg1	properties					332:341	weak mechanical properties	316:341	weak mechanical properties	316:341	However, the typical utility of borax and weak mechanical properties are not preferred either in the cell environment or as a supporting scaffold.					
33140191	10	0	from	applications	1558:1569	arg1	bioprinting					1590:1600	extrusion-based bioprinting	1574:1600	extrusion-based bioprinting	1574:1600	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	8	1	theme	hydrogel	1116:1123	arg1	biodegradation					1090:1103	biodegradation	1090:1103	biodegradation of Alg-Gel hydrogel	1090:1123	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	8	1	theme	hydrogel	1116:1123	arg1	structure					1076:1084	the highly porous structure	1058:1084	the highly porous structure	1058:1084	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	10	2	theme	bioink	1502:1507	arg1	reinforcement					1474:1486	the mechanical reinforcement	1459:1486	the mechanical reinforcement of cell-laden bioink	1459:1507	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	4	3	theme	Alg-Gel-BG	616:625	arg1	bioink					627:632	The composite Alg-Gel-BG bioink	602:632	The composite Alg-Gel-BG bioink	602:632	The composite Alg-Gel-BG bioink was biophysically characterized by mechanical tests and bioprinting practice.					
33140191	0	4	theme	glass	112:116	arg1	nanoparticles					118:130	bioactive glass nanoparticles	102:130	bioactive glass nanoparticles	102:130	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	4	5	theme	mechanical	669:678	arg1	tests					680:684	mechanical tests	669:684	mechanical tests	669:684	The composite Alg-Gel-BG bioink was biophysically characterized by mechanical tests and bioprinting practice.					
33140191	5	6	theme	Alg-Gel-BG	732:741	arg1	bioink					743:748	Alg-Gel-BG bioink	732:748	Alg-Gel-BG bioink	732:748	Biocompatibility of Alg-Gel-BG bioink was then investigated by bioprinting mouse dermal fibroblasts.					
33140191	10	7	theme	cell-laden	1491:1500	arg1	bioink					1502:1507	cell-laden bioink	1491:1507	cell-laden bioink	1491:1507	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	0	8	theme	bioactive	102:110	arg1	nanoparticles					118:130	bioactive glass nanoparticles	102:130	bioactive glass nanoparticles	102:130	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	5	9	theme	bioink	743:748	arg1	Biocompatibility					712:727	Biocompatibility	712:727	Biocompatibility of Alg-Gel-BG bioink	712:748	Biocompatibility of Alg-Gel-BG bioink was then investigated by bioprinting mouse dermal fibroblasts.					
33140191	3	10	theme	biological	579:588	arg1	properties					590:599	the mechanical and biological properties	560:599	the mechanical and biological properties	560:599	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	10	11	theme	incorporating	1374:1386	arg1	BG					1388:1389	incorporating BG	1374:1389	incorporating BG	1374:1389	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	3	12	dep	nanoparticles	451:463	arg1	size					480:483	particle size	471:483	particle size of 12 and 25 nm	471:499	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	8	13	theme	porous	1069:1074	arg1	structure					1076:1084	the highly porous structure	1058:1084	the highly porous structure	1058:1084	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	10	14	theme	future	1551:1556	arg1	applications					1558:1569	future applications	1551:1569	future applications in extrusion-based bioprinting	1551:1600	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	9	15	from	adhesion	1232:1239	arg1	constructs					1259:1268	the bioprinted constructs	1244:1268	the bioprinted constructs	1244:1268	In addition, the cellular proliferation and adhesion in the bioprinted constructs were significantly enhanced by BG (12 nm), while extension was not affected.					
33140191	8	16	theme	mechanical	1136:1145	arg1	strength					1147:1154	the mechanical strength	1132:1154	the mechanical strength	1132:1154	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	6	17	theme	BG	902:903	arg1	concentration					872:884	concentration	872:884	concentration of incorporated BG	872:903	Mechanical tests showed enhanced stiffness with increasing concentration of incorporated BG.					
33140191	3	18	theme	mechanical	564:573	arg1	properties					590:599	the mechanical and biological properties	560:599	the mechanical and biological properties	560:599	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	3	19	theme	bioactive	435:443	arg1	BG					466:467	BG	466:467	BG	466:467	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	3	19	theme	bioactive	435:443	arg1	nanoparticles					451:463	bioactive glass nanoparticles	435:463	bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm)	435:500	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	10	20	theme	extrusion-based	1574:1588	arg1	bioprinting					1590:1600	extrusion-based bioprinting	1574:1600	extrusion-based bioprinting	1574:1600	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	6	21	theme	incorporated	889:900	arg1	BG					902:903	incorporated BG	889:903	incorporated BG	889:903	Mechanical tests showed enhanced stiffness with increasing concentration of incorporated BG.					
33140191	7	22	theme	1.0 wt	957:962	arg1	%					963:963	1.0 wt%	957:963	1.0 wt%	957:963	But the maximum concentration of BG was determined 1.0 wt% before blends became too viscous to print.					
33140191	1	23	theme	extrusion-based	202:216	arg1	bioprinting					218:228	extrusion-based bioprinting	202:228	extrusion-based bioprinting	202:228	Alginate-gelatin (Alg-Gel) composite hydrogel is extensively used in extrusion-based bioprinting.					
33140191	3	24	theme	glass	445:449	arg1	BG					466:467	BG	466:467	BG	466:467	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	3	24	theme	glass	445:449	arg1	nanoparticles					451:463	bioactive glass nanoparticles	435:463	bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm)	435:500	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	8	25	theme	Alg-Gel	1108:1114	arg1	hydrogel					1116:1123	Alg-Gel hydrogel	1108:1123	Alg-Gel hydrogel	1108:1123	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	7	26	theme	BG	939:940	arg1	concentration					922:934	the maximum concentration	910:934	the maximum concentration of BG	910:940	But the maximum concentration of BG was determined 1.0 wt% before blends became too viscous to print.					
33140191	3	27	theme	particle	471:478	arg1	size					480:483	particle size	471:483	particle size of 12 and 25 nm	471:499	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	0	28	theme	mechanical	16:25	arg1	optimization					36:47	mechanical property optimization	16:47	mechanical property optimization of cell-laden alginate-gelatin composite	16:88	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	7	29	theme	maximum	914:920	arg1	concentration					922:934	the maximum concentration	910:934	the maximum concentration of BG	910:940	But the maximum concentration of BG was determined 1.0 wt% before blends became too viscous to print.					
33140191	5	30	theme	bioprinting	775:785	arg1	fibroblasts					800:810	bioprinting mouse dermal fibroblasts	775:810	bioprinting mouse dermal fibroblasts	775:810	Biocompatibility of Alg-Gel-BG bioink was then investigated by bioprinting mouse dermal fibroblasts.					
33140191	2	31	theme	clinical	361:368	arg1	applications					370:381	its further clinical applications	349:381	its further clinical applications	349:381	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	10	32	theme	Alg-Gel	1394:1400	arg1	hydrogel					1412:1419	Alg-Gel composite hydrogel	1394:1419	Alg-Gel composite hydrogel	1394:1419	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	3	33	theme	25 nm	495:499	arg1	size					480:483	particle size	471:483	particle size of 12 and 25 nm	471:499	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	8	34	theme	BG	1040:1041	arg1	incorporation					1023:1035	the incorporation	1019:1035	the incorporation of BG	1019:1041	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	8	34	theme	BG	1040:1041	arg1	Meanwhile					1008:1016	Meanwhile	1008:1016	Meanwhile	1008:1016	Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg-Gel hydrogel, while the mechanical strength and printability were enhanced.					
33140191	4	35	theme	composite	606:614	arg1	bioink					627:632	The composite Alg-Gel-BG bioink	602:632	The composite Alg-Gel-BG bioink	602:632	The composite Alg-Gel-BG bioink was biophysically characterized by mechanical tests and bioprinting practice.					
33140191	5	36	theme	mouse	787:791	arg1	fibroblasts					800:810	bioprinting mouse dermal fibroblasts	775:810	bioprinting mouse dermal fibroblasts	775:810	Biocompatibility of Alg-Gel-BG bioink was then investigated by bioprinting mouse dermal fibroblasts.					
33140191	2	37	theme	further	353:359	arg1	applications					370:381	its further clinical applications	349:381	its further clinical applications	349:381	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	10	38	theme	composite	1402:1410	arg1	hydrogel					1412:1419	Alg-Gel composite hydrogel	1394:1419	Alg-Gel composite hydrogel	1394:1419	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	5	39	theme	dermal	793:798	arg1	fibroblasts					800:810	bioprinting mouse dermal fibroblasts	775:810	bioprinting mouse dermal fibroblasts	775:810	Biocompatibility of Alg-Gel-BG bioink was then investigated by bioprinting mouse dermal fibroblasts.					
33140191	9	40	from	proliferation	1214:1226	arg1	constructs					1259:1268	the bioprinted constructs	1244:1268	the bioprinted constructs	1244:1268	In addition, the cellular proliferation and adhesion in the bioprinted constructs were significantly enhanced by BG (12 nm), while extension was not affected.					
33140191	10	41	from	strategy	1362:1369	arg1	hydrogel					1412:1419	Alg-Gel composite hydrogel	1394:1419	Alg-Gel composite hydrogel	1394:1419	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	9	42	theme	cellular	1205:1212	arg1	proliferation					1214:1226	cellular proliferation	1205:1226	cellular proliferation	1205:1226	In addition, the cellular proliferation and adhesion in the bioprinted constructs were significantly enhanced by BG (12 nm), while extension was not affected.					
33140191	3	43	theme	12	488:489	arg1	size					480:483	particle size	471:483	particle size of 12 and 25 nm	471:499	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	0	44	theme	property	27:34	arg1	optimization					36:47	mechanical property optimization	16:47	mechanical property optimization of cell-laden alginate-gelatin composite	16:88	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	10	45	theme	easy-to-use	1435:1445	arg1	approach					1447:1454	an easy-to-use approach	1432:1454	an easy-to-use approach to the mechanical reinforcement of cell-laden bioink	1432:1507	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	9	46	theme	bioprinted	1248:1257	arg1	constructs					1259:1268	the bioprinted constructs	1244:1268	the bioprinted constructs	1244:1268	In addition, the cellular proliferation and adhesion in the bioprinted constructs were significantly enhanced by BG (12 nm), while extension was not affected.					
33140191	0	47	theme	cell-laden	52:61	arg1	composite					80:88	cell-laden alginate-gelatin composite	52:88	cell-laden alginate-gelatin composite	52:88	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	10	48	theme	BG	1388:1389	arg1	strategy					1362:1369	our strategy	1358:1369	our strategy of incorporating BG in Alg-Gel composite hydrogel	1358:1419	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	2	49	theme	excellent	263:271	arg1	biocompatibility					273:288	excellent biocompatibility	263:288	excellent biocompatibility	263:288	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	2	50	theme	Alg-Gel	240:246	arg1	blends					248:253	Alg-Gel blends	240:253	Alg-Gel blends	240:253	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	2	51	theme	mechanical	313:322	arg1	properties					324:333	poor mechanical properties	308:333	poor mechanical properties	308:333	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	6	52	theme	enhanced	837:844	arg1	stiffness					846:854	enhanced stiffness	837:854	enhanced stiffness	837:854	Mechanical tests showed enhanced stiffness with increasing concentration of incorporated BG.					
33140191	2	53	contain	possess	255:261	arg2	biocompatibility					273:288	excellent biocompatibility	263:288	excellent biocompatibility	263:288	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	2	53	contain	possess	255:261	arg2	printability					294:305	printability	294:305	printability	294:305	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	2	53	contain	possess	255:261	arg1	blends					248:253	Alg-Gel blends	240:253	Alg-Gel blends	240:253	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	2	54	theme	poor	308:311	arg1	properties					324:333	poor mechanical properties	308:333	poor mechanical properties	308:333	Although Alg-Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications.					
33140191	10	55	theme	mechanical	1463:1472	arg1	reinforcement					1474:1486	the mechanical reinforcement	1459:1486	the mechanical reinforcement of cell-laden bioink	1459:1507	Therefore, our strategy of incorporating BG in Alg-Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.					
33140191	3	56	theme	Alg-Gel	507:513	arg1	hydrogel					515:522	Alg-Gel hydrogel	507:522	Alg-Gel hydrogel	507:522	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33140191	0	57	theme	composite	80:88	arg1	optimization					36:47	mechanical property optimization	16:47	mechanical property optimization of cell-laden alginate-gelatin composite	16:88	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	4	58	theme	bioprinting	690:700	arg1	practice					702:709	bioprinting practice	690:709	bioprinting practice	690:709	The composite Alg-Gel-BG bioink was biophysically characterized by mechanical tests and bioprinting practice.					
33140191	1	59	theme	composite	160:168	arg1	hydrogel					170:177	Alginate-gelatin (Alg-Gel) composite hydrogel	133:177	Alginate-gelatin (Alg-Gel) composite hydrogel	133:177	Alginate-gelatin (Alg-Gel) composite hydrogel is extensively used in extrusion-based bioprinting.					
33140191	0	60	theme	alginate-gelatin	63:78	arg1	composite					80:88	cell-laden alginate-gelatin composite	52:88	cell-laden alginate-gelatin composite	52:88	An approach for mechanical property optimization of cell-laden alginate-gelatin composite bioink with bioactive glass nanoparticles.					
33140191	1	61	used	used	194:197	arg2	hydrogel					170:177	Alginate-gelatin (Alg-Gel) composite hydrogel	133:177	Alginate-gelatin (Alg-Gel) composite hydrogel	133:177	Alginate-gelatin (Alg-Gel) composite hydrogel is extensively used in extrusion-based bioprinting.					
33140191	6	62	theme	Mechanical	813:822	arg1	tests					824:828	Mechanical tests	813:828	Mechanical tests	813:828	Mechanical tests showed enhanced stiffness with increasing concentration of incorporated BG.					
33140191	3	63	theme	design	411:416	arg1	series					401:406	a series	399:406	a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel	399:522	In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg-Gel hydrogel have been considered for optimizing the mechanical and biological properties.					
33593536	4	0	theme	Cs-0.25	518:524	arg1	%					535:535	The 2 %Cs-0.25 %PA-0.25 %	511:535	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	511:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	6	1	theme	material	1043:1050	arg1	retention					1052:1060	material retention	1043:1060	material retention	1043:1060	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	5	2	theme	%	788:788	arg1	hydrogel					802:809	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	7	3	theme	pressure	1256:1263	arg1	sites					1274:1278	low pressure bleeding sites	1252:1278	low pressure bleeding sites	1252:1278	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	7	4	theme	hemostatic	1206:1215	arg1	agent					1217:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	2	5	theme	activator-calcium	302:318	arg1	Ca					336:337	0.25 %Ca	330:337	0.25 %Ca	330:337	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	5	theme	activator-calcium	302:318	arg1	chloride					320:327	coagulation activator-calcium chloride	290:327	coagulation activator-calcium chloride (0.25 %Ca)	290:338	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	5	6	theme	composite	792:800	arg1	hydrogel					802:809	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	7	7	theme	hydrogel	1191:1198	arg1	agent					1217:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	4	8	theme	rapid	565:569	arg1	clotting					577:584	rapid blood clotting	565:584	rapid blood clotting	565:584	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	5	9	theme	s	933:933	arg1	±					928:928	77 ±	925:928	77 ± 26 s	925:933	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	9	theme	s	933:933	arg1	sealant					916:922	commercial hemostatic agents-Fibrin sealant	880:922	commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s)	880:946	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	10	theme	%	770:770	arg1	%					788:788	2 %Cs-0.25 %PA-0.25 %	768:788	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	7	11	theme	prepared	1172:1179	arg1	agent					1217:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	5	12	theme	204	936:938	arg1	s					945:945	204 ± 58 s	936:945	204 ± 58 s	936:945	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	13	theme	%	779:779	arg1	%					788:788	2 %Cs-0.25 %PA-0.25 %	768:788	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	4	14	theme	cascade	661:667	arg1	activation					631:640	activation	631:640	activation of the coagulation cascade	631:667	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	4	14	theme	cascade	661:667	arg1	aggregation					615:625	RBC/platelet aggregation	602:625	RBC/platelet aggregation	602:625	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	6	15	theme	post-surgery	1081:1092	arg1	weeks					1075:1079	8 weeks post-surgery	1073:1092	8 weeks post-surgery	1073:1092	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	2	16	theme	sulfate	267:273	arg1	entrapment					217:226	entrapment	217:226	entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel	217:361	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	5	17	theme	rat	698:700	arg1	liver					702:706	rat liver	698:706	rat liver	698:706	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	2	18	theme	vasoconstrictor-potassium	231:255	arg1	PA					282:283	0.25 %PA	276:283	0.25 %PA	276:283	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	18	theme	vasoconstrictor-potassium	231:255	arg1	sulfate					267:273	vasoconstrictor-potassium aluminium sulfate	231:273	vasoconstrictor-potassium aluminium sulfate (0.25 %PA)	231:284	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	0	19	theme	rapid	90:94	arg1	control					105:111	rapid bleeding control	90:111	rapid bleeding control	90:111	Vasoconstrictor and coagulation activator entrapped chitosan based composite hydrogel for rapid bleeding control.					
33593536	7	20	from	application	1237:1247	arg1	sites					1274:1278	low pressure bleeding sites	1252:1278	low pressure bleeding sites	1252:1278	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	5	21	theme	in	679:680	arg1	studies					687:693	in vivo studies	679:693	in vivo studies on rat liver	679:706	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	22	theme	±	867:867	arg1	s					872:872	105 ± 31 s	863:872	105 ± 31 s	863:872	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	22	theme	±	867:867	arg1	s					860:860	20 ± 10 s	852:860	20 ± 10 s	852:860	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	3	23	theme	shear	470:474	arg1	cyto					486:489	cyto	486:489	cyto	486:489	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	23	theme	shear	470:474	arg1	injectable					458:467	injectable	458:467	injectable	458:467	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	23	theme	shear	470:474	arg1	thinning					476:483	shear thinning	470:483	shear thinning	470:483	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	23	theme	shear	470:474	arg1	hydrogel					445:452	the prepared composite hydrogel	422:452	the prepared composite hydrogel	422:452	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	23	theme	shear	470:474	arg1	hemocompatible					495:508	hemocompatible	495:508	hemocompatible	495:508	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	5	24	theme	±	976:976	arg1	s					981:981	218 ± 46 s	972:981	218 ± 46 s	972:981	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	24	theme	±	976:976	arg1	s					969:969	76 ± 15 s	961:969	76 ± 15 s	961:969	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	4	25	dep	%	535:535	arg1	hydrogel					549:556	Ca composite hydrogel	536:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	511:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	3	26	dep	injectable	458:467	arg1	cyto					486:489	cyto	486:489	cyto	486:489	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	26	dep	injectable	458:467	arg1	injectable					458:467	injectable	458:467	injectable	458:467	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	26	dep	injectable	458:467	arg1	thinning					476:483	shear thinning	470:483	shear thinning	470:483	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	26	dep	injectable	458:467	arg1	hydrogel					445:452	the prepared composite hydrogel	422:452	the prepared composite hydrogel	422:452	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	26	dep	injectable	458:467	arg1	hemocompatible					495:508	hemocompatible	495:508	hemocompatible	495:508	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	2	27	theme	hemostatic	381:390	arg1	property					392:399	the hemostatic property	377:399	the hemostatic property of Cs	377:405	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	0	28	theme	coagulation	20:30	arg1	chitosan					52:59	coagulation activator entrapped chitosan	20:59	coagulation activator entrapped chitosan	20:59	Vasoconstrictor and coagulation activator entrapped chitosan based composite hydrogel for rapid bleeding control.					
33593536	1	29	theme	hemostatic	133:142	arg1	agent					144:148	a hemostatic agent	131:148	a hemostatic agent	131:148	Chitosan (Cs) as a hemostatic agent has been in use to control hemorrage.					
33593536	5	30	dep	±	928:928	arg1	s					945:945	204 ± 58 s	936:945	204 ± 58 s	936:945	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	0	31	theme	entrapped	42:50	arg1	chitosan					52:59	coagulation activator entrapped chitosan	20:59	coagulation activator entrapped chitosan	20:59	Vasoconstrictor and coagulation activator entrapped chitosan based composite hydrogel for rapid bleeding control.					
33593536	5	32	theme	artery	720:725	arg1	model					737:741	femoral artery hemorrage model	712:741	femoral artery hemorrage model	712:741	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	33	theme	hemostatic	891:900	arg1	±					928:928	77 ±	925:928	77 ± 26 s	925:933	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	33	theme	hemostatic	891:900	arg1	sealant					916:922	commercial hemostatic agents-Fibrin sealant	880:922	commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s)	880:946	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	3	34	theme	composite	435:443	arg1	cyto					486:489	cyto	486:489	cyto	486:489	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	34	theme	composite	435:443	arg1	injectable					458:467	injectable	458:467	injectable	458:467	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	34	theme	composite	435:443	arg1	thinning					476:483	shear thinning	470:483	shear thinning	470:483	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	34	theme	composite	435:443	arg1	hydrogel					445:452	the prepared composite hydrogel	422:452	the prepared composite hydrogel	422:452	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	34	theme	composite	435:443	arg1	hemocompatible					495:508	hemocompatible	495:508	hemocompatible	495:508	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	2	35	theme	Composite	188:196	arg1	hydrogel					198:205	Composite hydrogel	188:205	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel	188:361	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	36	theme	0.25	330:333	arg1	%					335:335	%	335:335	%	335:335	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	5	37	theme	hemorrage	727:735	arg1	model					737:741	femoral artery hemorrage model	712:741	femoral artery hemorrage model	712:741	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	4	38	theme	composite	539:547	arg1	hydrogel					549:556	Ca composite hydrogel	536:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	511:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	4	39	theme	%	517:517	arg1	%					535:535	The 2 %Cs-0.25 %PA-0.25 %	511:535	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	511:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	3	40	from	injectable	458:467	arg1	work					416:419	this work	411:419	this work	411:419	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	7	41	theme	bleeding	1265:1272	arg1	sites					1274:1278	low pressure bleeding sites	1252:1278	low pressure bleeding sites	1252:1278	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	4	42	theme	%	526:526	arg1	%					535:535	The 2 %Cs-0.25 %PA-0.25 %	511:535	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	511:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	2	43	theme	%	281:281	arg1	PA					282:283	0.25 %PA	276:283	0.25 %PA	276:283	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	43	theme	%	281:281	arg1	sulfate					267:273	vasoconstrictor-potassium aluminium sulfate	231:273	vasoconstrictor-potassium aluminium sulfate (0.25 %PA)	231:284	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	7	44	theme	low	1252:1254	arg1	sites					1274:1278	low pressure bleeding sites	1252:1278	low pressure bleeding sites	1252:1278	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	7	45	theme	potential	1227:1235	arg1	application					1237:1247	potential application	1227:1247	potential application in low pressure bleeding sites	1227:1278	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	2	46	theme	chloride	320:327	arg1	entrapment					217:226	entrapment	217:226	entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel	217:361	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	5	47	theme	Ca	789:790	arg1	hydrogel					802:809	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	4	48	theme	RBC/platelet	602:613	arg1	aggregation					615:625	RBC/platelet aggregation	602:625	RBC/platelet aggregation	602:625	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	2	49	theme	coagulation	290:300	arg1	Ca					336:337	0.25 %Ca	330:337	0.25 %Ca	330:337	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	49	theme	coagulation	290:300	arg1	chloride					320:327	coagulation activator-calcium chloride	290:327	coagulation activator-calcium chloride (0.25 %Ca)	290:338	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	7	50	theme	based	1200:1204	arg1	agent					1217:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	5	51	from	studies	687:693	arg1	liver					702:706	rat liver	698:706	rat liver	698:706	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	52	theme	Cs-0.25	771:777	arg1	%					788:788	2 %Cs-0.25 %PA-0.25 %	768:788	2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	768:809	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	7	53	theme	composite	1181:1189	arg1	agent					1217:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	4	54	theme	blood	571:575	arg1	clotting					577:584	rapid blood clotting	565:584	rapid blood clotting	565:584	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	6	55	theme	excess	1105:1110	arg1	hydrogel					1112:1119	excess hydrogel	1105:1119	excess hydrogel	1105:1119	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	5	56	theme	±	940:940	arg1	s					945:945	204 ± 58 s	936:945	204 ± 58 s	936:945	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	6	57	theme	toxicological	996:1008	arg1	study					1010:1014	in vivo toxicological study	988:1014	in vivo toxicological study	988:1014	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	4	58	theme	coagulation	649:659	arg1	cascade					661:667	the coagulation cascade	645:667	the coagulation cascade	645:667	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	5	59	theme	shorter	838:844	arg1	time					846:849	a shorter time	836:849	a shorter time (20 ± 10 s, 105 ± 31 s)	836:873	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	60	dep	in	679:680	arg1	vivo					682:685	vivo	682:685	vivo	682:685	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	6	61	theme	in	988:989	arg1	study					1010:1014	in vivo toxicological study	988:1014	in vivo toxicological study	988:1014	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	6	62	dep	in	988:989	arg1	vivo					991:994	vivo	991:994	vivo	991:994	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	5	63	theme	26	930:931	arg1	s					933:933	s	933:933	s	933:933	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	2	64	theme	aluminium	257:265	arg1	PA					282:283	0.25 %PA	276:283	0.25 %PA	276:283	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	64	theme	aluminium	257:265	arg1	sulfate					267:273	vasoconstrictor-potassium aluminium sulfate	231:273	vasoconstrictor-potassium aluminium sulfate (0.25 %PA)	231:284	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	5	65	theme	hydrogel	802:809	arg1	efficiency					754:763	the efficiency	750:763	the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s)	750:982	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	0	66	theme	bleeding	96:103	arg1	control					105:111	rapid bleeding control	90:111	rapid bleeding control	90:111	Vasoconstrictor and coagulation activator entrapped chitosan based composite hydrogel for rapid bleeding control.					
33593536	5	67	from	model	737:741	arg1	liver					702:706	rat liver	698:706	rat liver	698:706	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	6	68	theme	application	1154:1164	arg1	application					1154:1164	application	1154:1164	application	1154:1164	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	6	68	theme	application	1154:1164	arg1	site					1146:1149	site	1146:1149	site of application	1146:1164	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	5	69	theme	±	964:964	arg1	s					981:981	218 ± 46 s	972:981	218 ± 46 s	972:981	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	69	theme	±	964:964	arg1	s					969:969	76 ± 15 s	961:969	76 ± 15 s	961:969	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	70	dep	time	846:849	arg1	s					872:872	105 ± 31 s	863:872	105 ± 31 s	863:872	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	70	dep	time	846:849	arg1	s					860:860	20 ± 10 s	852:860	20 ± 10 s	852:860	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	2	71	theme	Cs	404:405	arg1	property					392:399	the hemostatic property	377:399	the hemostatic property of Cs	377:405	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	0	72	theme	activator	32:40	arg1	chitosan					52:59	coagulation activator entrapped chitosan	20:59	coagulation activator entrapped chitosan	20:59	Vasoconstrictor and coagulation activator entrapped chitosan based composite hydrogel for rapid bleeding control.					
33593536	5	73	theme	±	855:855	arg1	s					872:872	105 ± 31 s	863:872	105 ± 31 s	863:872	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	73	theme	±	855:855	arg1	s					860:860	20 ± 10 s	852:860	20 ± 10 s	852:860	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	6	74	theme	composite	1017:1025	arg1	hydrogel					1027:1034	composite hydrogel	1017:1034	composite hydrogel	1017:1034	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	5	75	dep	Floseal	952:958	arg1	s					981:981	218 ± 46 s	972:981	218 ± 46 s	972:981	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	75	dep	Floseal	952:958	arg1	s					969:969	76 ± 15 s	961:969	76 ± 15 s	961:969	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	76	theme	femoral	712:718	arg1	model					737:741	femoral artery hemorrage model	712:741	femoral artery hemorrage model	712:741	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	77	theme	commercial	880:889	arg1	±					928:928	77 ±	925:928	77 ± 26 s	925:933	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	77	theme	commercial	880:889	arg1	sealant					916:922	commercial hemostatic agents-Fibrin sealant	880:922	commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s)	880:946	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	0	78	theme	composite	67:75	arg1	hydrogel					77:84	composite hydrogel	67:84	composite hydrogel for rapid bleeding control	67:111	Vasoconstrictor and coagulation activator entrapped chitosan based composite hydrogel for rapid bleeding control.					
33593536	7	79	contain	has	1223:1225	arg2	application					1237:1247	potential application	1227:1247	potential application in low pressure bleeding sites	1227:1278	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	7	79	contain	has	1223:1225	arg1	agent					1217:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent	1167:1221	This prepared composite hydrogel based hemostatic agent has potential application in low pressure bleeding sites.					
33593536	5	80	theme	agents-Fibrin	902:914	arg1	±					928:928	77 ±	925:928	77 ± 26 s	925:933	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	5	80	theme	agents-Fibrin	902:914	arg1	sealant					916:922	commercial hemostatic agents-Fibrin sealant	880:922	commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s)	880:946	Further, in vivo studies on rat liver and femoral artery hemorrage model showed the efficiency of 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel to achieve hemostasis in a shorter time (20 ± 10 s, 105 ± 31 s) than commercial hemostatic agents-Fibrin sealant (77 ± 26 s, 204 ± 58 s) and Floseal (76 ± 15 s, 218 ± 46 s).					
33593536	3	81	from	work	416:419	arg1	cyto					486:489	cyto	486:489	cyto	486:489	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	81	from	work	416:419	arg1	injectable					458:467	injectable	458:467	injectable	458:467	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	81	from	work	416:419	arg1	thinning					476:483	shear thinning	470:483	shear thinning	470:483	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	81	from	work	416:419	arg1	hydrogel					445:452	the prepared composite hydrogel	422:452	the prepared composite hydrogel	422:452	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	81	from	work	416:419	arg1	hemocompatible					495:508	hemocompatible	495:508	hemocompatible	495:508	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	2	82	theme	%	335:335	arg1	Ca					336:337	0.25 %Ca	330:337	0.25 %Ca	330:337	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	2	82	theme	%	335:335	arg1	chloride					320:327	coagulation activator-calcium chloride	290:327	coagulation activator-calcium chloride (0.25 %Ca)	290:338	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
33593536	4	83	theme	Ca	536:537	arg1	hydrogel					549:556	Ca composite hydrogel	536:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel	511:556	The 2 %Cs-0.25 %PA-0.25 %Ca composite hydrogel caused rapid blood clotting by accelerating RBC/platelet aggregation and activation of the coagulation cascade.					
33593536	3	84	theme	prepared	426:433	arg1	cyto					486:489	cyto	486:489	cyto	486:489	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	84	theme	prepared	426:433	arg1	injectable					458:467	injectable	458:467	injectable	458:467	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	84	theme	prepared	426:433	arg1	thinning					476:483	shear thinning	470:483	shear thinning	470:483	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	84	theme	prepared	426:433	arg1	hydrogel					445:452	the prepared composite hydrogel	422:452	the prepared composite hydrogel	422:452	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	3	84	theme	prepared	426:433	arg1	hemocompatible					495:508	hemocompatible	495:508	hemocompatible	495:508	In this work, the prepared composite hydrogel was injectable, shear thinning, cyto and hemocompatible.					
33593536	6	85	dep	showed	1036:1041	arg1	irrigated					1131:1139	irrigated	1131:1139	should be irrigated from site of application	1121:1164	In in vivo toxicological study, composite hydrogel showed material retention even after 8 weeks post-surgery, therefore excess hydrogel should be irrigated from site of application.					
33593536	2	86	theme	Cs	345:346	arg1	hydrogel					354:361	Cs (2 %) hydrogel	345:361	Cs (2 %) hydrogel	345:361	Composite hydrogel formed by entrapment of vasoconstrictor-potassium aluminium sulfate (0.25 %PA) and coagulation activator-calcium chloride (0.25 %Ca) into Cs (2 %) hydrogel would enhance the hemostatic property of Cs.					
32794755	8	0	theme	starch	1137:1142	arg1	accumulation					1144:1155	starch accumulation	1137:1155	starch accumulation	1137:1155	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	2	1	theme	quality	344:350	arg1	contents					274:281	The contents	270:281	The contents	270:281	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	1	theme	quality	344:350	arg1	traits					352:357	the significant quality traits	328:357	the significant quality traits of C. moschata	328:372	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	1	theme	quality	344:350	arg1	composition					284:294	composition	284:294	composition	284:294	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	1	theme	quality	344:350	arg1	conformation					301:312	conformation	301:312	conformation	301:312	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	6	2	theme	key	933:935	arg1	gene					937:940	each key gene	928:940	each key gene involved in the pathway	928:964	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	2	3	theme	significant	332:342	arg1	contents					274:281	The contents	270:281	The contents	270:281	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	3	theme	significant	332:342	arg1	traits					352:357	the significant quality traits	328:357	the significant quality traits of C. moschata	328:372	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	3	theme	significant	332:342	arg1	composition					284:294	composition	284:294	composition	284:294	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	3	theme	significant	332:342	arg1	conformation					301:312	conformation	301:312	conformation	301:312	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	8	4	theme	important	1181:1189	arg1	factor					1191:1196	an important factor	1178:1196	an important factor for the determination of fruit quality	1178:1235	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	8	4	theme	important	1181:1189	arg1	regulation					1101:1110	the developmental regulation	1083:1110	the developmental regulation of genes associated with starch accumulation	1083:1155	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	4	5	theme	amylose/amylopectin	502:520	arg1	ratio					522:526	amylose/amylopectin ratio	502:526	amylose/amylopectin ratio	502:526	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	1	6	theme	several	252:258	arg1	diseases					260:267	several diseases	252:267	several diseases	252:267	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	5	7	theme	starch	759:764	arg1	accumulation					766:777	the starch accumulation	755:777	the starch accumulation	755:777	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	5	8	theme	Scanning	605:612	arg1	microscopy					623:632	Scanning electron microscopy	605:632	Scanning electron microscopy	605:632	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	4	9	theme	fruit	580:584	arg1	stage					598:602	each fruit development stage	575:602	each fruit development stage	575:602	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	5	10	theme	electron	614:621	arg1	microscopy					623:632	Scanning electron microscopy	605:632	Scanning electron microscopy	605:632	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	6	11	from	CMO-X	831:835	arg1	comparison					786:795	a comparison	784:795	a comparison of fruit development in CMO-E and CMO-X	784:835	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	1	12	theme	diseases	260:267	arg1	treatment					239:247	the treatment	235:247	the treatment of several diseases	235:267	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	4	13	theme	starch	482:487	arg1	high					533:536	high	533:536	high	533:536	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	4	13	theme	starch	482:487	arg1	contents					489:496	the total starch contents	472:496	the total starch contents	472:496	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	4	14	from	CMO-X	541:545	arg1	high					533:536	high	533:536	high	533:536	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	4	14	from	CMO-X	541:545	arg1	contents					489:496	the total starch contents	472:496	the total starch contents	472:496	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	3	15	theme	taste	431:435	arg1	difference					437:446	the taste difference	427:446	the taste difference	427:446	Two germplasms were targeted for analysis regarding the taste difference.					
32794755	4	16	theme	total	476:480	arg1	high					533:536	high	533:536	high	533:536	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	4	16	theme	total	476:480	arg1	contents					489:496	the total starch contents	472:496	the total starch contents	472:496	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	5	17	theme	transmission	638:649	arg1	microscopy					660:669	transmission electron microscopy	638:669	transmission electron microscopy	638:669	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	7	18	theme	CMO-X	1062:1066	arg1	ratio					1043:1047	the amylose/amylopectin ratio	1019:1047	the amylose/amylopectin ratio of CMO-E and CMO-X	1019:1066	GBSS and SBE were correlated with the difference in the amylose/amylopectin ratio of CMO-E and CMO-X.					
32794755	2	19	theme	moschata	365:372	arg1	contents					274:281	The contents	270:281	The contents	270:281	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	19	theme	moschata	365:372	arg1	traits					352:357	the significant quality traits	328:357	the significant quality traits of C. moschata	328:372	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	19	theme	moschata	365:372	arg1	composition					284:294	composition	284:294	composition	284:294	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	19	theme	moschata	365:372	arg1	conformation					301:312	conformation	301:312	conformation	301:312	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	5	20	theme	electron	651:658	arg1	microscopy					660:669	transmission electron microscopy	638:669	transmission electron microscopy	638:669	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	6	21	theme	development	806:816	arg1	comparison					786:795	a comparison	784:795	a comparison of fruit development in CMO-E and CMO-X	784:835	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	1	22	theme	Cucurbita	100:108	arg1	moschata					110:117	Cucurbita moschata	100:117	Cucurbita moschata; Cucurbitaceae	100:132	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	7	23	from	difference	1005:1014	arg1	ratio					1043:1047	the amylose/amylopectin ratio	1019:1047	the amylose/amylopectin ratio of CMO-E and CMO-X	1019:1066	GBSS and SBE were correlated with the difference in the amylose/amylopectin ratio of CMO-E and CMO-X.					
32794755	8	24	theme	fruit	1223:1227	arg1	quality					1229:1235	fruit quality	1223:1235	fruit quality	1223:1235	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	6	25	theme	fruit	800:804	arg1	development					806:816	fruit development	800:816	fruit development in CMO-E and CMO-X	800:835	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	1	26	dep	moschata	110:117	arg1	Cucurbitaceae					120:132	Cucurbitaceae	120:132	Cucurbita moschata; Cucurbitaceae	100:132	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	6	27	from	development	806:816	arg1	CMO-X					831:835	CMO-X	831:835	CMO-X	831:835	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	6	27	from	development	806:816	arg1	CMO-E					821:825	CMO-E	821:825	CMO-E	821:825	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	0	28	theme	Starch	20:25	arg1	Characterization					0:15	Characterization	0:15	Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.	0:88	Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.					
32794755	6	29	theme	starch	863:868	arg1	metabolism					870:879	starch metabolism	863:879	starch metabolism	863:879	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	8	30	theme	developmental	1087:1099	arg1	factor					1191:1196	an important factor	1178:1196	an important factor for the determination of fruit quality	1178:1235	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	8	30	theme	developmental	1087:1099	arg1	regulation					1101:1110	the developmental regulation	1083:1110	the developmental regulation of genes associated with starch accumulation	1083:1155	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	1	31	theme	nutrients	158:166	arg1	source					148:153	the rich source	139:153	the rich source of nutrients	139:166	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	1	31	theme	nutrients	158:166	arg1	Pumpkins					90:97	Pumpkins	90:97	Pumpkins (Cucurbita moschata; Cucurbitaceae)	90:133	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	1	32	theme	active	202:207	arg1	substances					209:218	their biologically active substances	183:218	their biologically active substances	183:218	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	0	33	theme	moschata	40:47	arg1	Germplasms					49:58	Cucurbita moschata Germplasms	30:58	Cucurbita moschata Germplasms throughout Fruit Development	30:87	Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.					
32794755	8	34	theme	genes	1115:1119	arg1	factor					1191:1196	an important factor	1178:1196	an important factor for the determination of fruit quality	1178:1235	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	8	34	theme	genes	1115:1119	arg1	regulation					1101:1110	the developmental regulation	1083:1110	the developmental regulation of genes associated with starch accumulation	1083:1155	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	6	35	from	CMO-E	821:825	arg1	comparison					786:795	a comparison	784:795	a comparison of fruit development in CMO-E and CMO-X	784:835	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	0	36	theme	Cucurbita	30:38	arg1	Germplasms					49:58	Cucurbita moschata Germplasms	30:58	Cucurbita moschata Germplasms throughout Fruit Development	30:87	Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.					
32794755	8	37	theme	quality	1229:1235	arg1	determination					1206:1218	the determination	1202:1218	the determination of fruit quality	1202:1235	Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.					
32794755	7	38	theme	amylose/amylopectin	1023:1041	arg1	ratio					1043:1047	the amylose/amylopectin ratio	1019:1047	the amylose/amylopectin ratio of CMO-E and CMO-X	1019:1066	GBSS and SBE were correlated with the difference in the amylose/amylopectin ratio of CMO-E and CMO-X.					
32794755	4	39	from	high	533:536	arg1	CMO-X					541:545	CMO-X	541:545	CMO-X	541:545	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	5	40	theme	smooth	698:703	arg1	granules					720:727	smooth surface starch granules	698:727	smooth surface starch granules	698:727	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	0	41	from	Characterization	0:15	arg1	Germplasms					49:58	Cucurbita moschata Germplasms	30:58	Cucurbita moschata Germplasms throughout Fruit Development	30:87	Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.					
32794755	5	42	theme	surface	705:711	arg1	granules					720:727	smooth surface starch granules	698:727	smooth surface starch granules	698:727	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	4	43	theme	development	586:596	arg1	stage					598:602	each fruit development stage	575:602	each fruit development stage	575:602	Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage.					
32794755	1	44	dep	Pumpkins	90:97	arg1	moschata					110:117	Cucurbita moschata	100:117	Cucurbita moschata; Cucurbitaceae	100:132	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	5	45	theme	starch	713:718	arg1	granules					720:727	smooth surface starch granules	698:727	smooth surface starch granules	698:727	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	2	46	theme	starch	317:322	arg1	contents					274:281	The contents	270:281	The contents	270:281	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	46	theme	starch	317:322	arg1	traits					352:357	the significant quality traits	328:357	the significant quality traits of C. moschata	328:372	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	46	theme	starch	317:322	arg1	composition					284:294	composition	284:294	composition	284:294	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	2	46	theme	starch	317:322	arg1	conformation					301:312	conformation	301:312	conformation	301:312	The contents, composition, and conformation of starch are the significant quality traits of C. moschata.					
32794755	7	47	theme	CMO-E	1052:1056	arg1	ratio					1043:1047	the amylose/amylopectin ratio	1019:1047	the amylose/amylopectin ratio of CMO-E and CMO-X	1019:1066	GBSS and SBE were correlated with the difference in the amylose/amylopectin ratio of CMO-E and CMO-X.					
32794755	1	48	used	used	226:229	arg2	source					148:153	the rich source	139:153	the rich source of nutrients	139:166	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	1	48	used	used	226:229	arg2	Pumpkins					90:97	Pumpkins	90:97	Pumpkins (Cucurbita moschata; Cucurbitaceae)	90:133	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	6	49	from	comparison	786:795	arg1	CMO-X					831:835	CMO-X	831:835	CMO-X	831:835	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	6	49	from	comparison	786:795	arg1	CMO-E					821:825	CMO-E	821:825	CMO-E	821:825	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	5	50	dep	microscopy	623:632	arg1	observations					671:682	observations	671:682	observations	671:682	Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation.					
32794755	6	51	theme	putative	842:849	arg1	pathway					851:857	the putative pathway	838:857	the putative pathway for starch metabolism	838:879	For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway.					
32794755	1	52	theme	rich	143:146	arg1	source					148:153	the rich source	139:153	the rich source of nutrients	139:166	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	1	52	theme	rich	143:146	arg1	Pumpkins					90:97	Pumpkins	90:97	Pumpkins (Cucurbita moschata; Cucurbitaceae)	90:133	Pumpkins (Cucurbita moschata; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases.					
32794755	0	53	theme	Fruit	71:75	arg1	Development					77:87	Fruit Development	71:87	Fruit Development	71:87	Characterization of Starch in Cucurbita moschata Germplasms throughout Fruit Development.					
32171895	10	0	theme	partial	1789:1795	arg1	analysis					1824:1831	orthogonal partial least squares discriminant analysis	1778:1831	orthogonal partial least squares discriminant analysis (OPLS-DA)	1778:1841	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	0	theme	partial	1789:1795	arg1	OPLS-DA					1834:1840	OPLS-DA	1834:1840	OPLS-DA	1834:1840	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	9	1	with	samples	1495:1501	arg1	activity					1524:1531	neuroprotective activity	1508:1531	neuroprotective activity	1508:1531	An untargeted metabolomics approach was developed to explore the chemical composition of samples with neuroprotective activity.					
32171895	18	2	theme	neuroprotective	2861:2875	arg1	activity					2877:2884	sufficient neuroprotective activity	2850:2884	sufficient neuroprotective activity by in vitro assay	2850:2902	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	7	3	dep	MATERIAL	970:977	arg1	cytotoxicity					995:1006	The cytotoxicity	991:1006	The cytotoxicity	991:1006	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	4	theme	fractions	1042:1050	arg1	neuroprotection					1091:1105	neuroprotection	1091:1105	neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1091:1195	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	4	theme	fractions	1042:1050	arg1	METHODS					983:989	METHODS	983:989	METHODS	983:989	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	4	theme	fractions	1042:1050	arg1	MATERIAL					970:977	MATERIAL	970:977	MATERIAL	970:977	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	6	5	theme	beverage	767:774	arg1	potential					740:748	the neuroprotective potential	720:748	the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model	720:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	10	6	theme	metabolome	1643:1652	arg1	data					1654:1657	Time-of-Flight (UPLC-ESI-TOF) metabolome data	1613:1657	Time-of-Flight (UPLC-ESI-TOF) metabolome data	1613:1657	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	20	7	theme	isolated	3383:3390	arg1	alkaloids					3392:3400	isolated alkaloids	3383:3400	isolated alkaloids	3383:3400	Intriguingly, the hydroalcoholic fractions exhibited enhanced neuroprotective effects when compared to other samples and isolated alkaloids.					
32171895	14	8	theme	neuroprotective	2272:2286	arg1	effect					2288:2293	this neuroprotective effect	2267:2293	this neuroprotective effect	2267:2293	The crude extracts, alkaloid fractions and HRE demonstrated remarkable neuroprotective effect at T2 while the hydroalcoholic fractions demonstrated this neuroprotective effect at T1 and T2.					
32171895	10	9	theme	UPLC-ESI-TOF	1629:1640	arg1	data					1654:1657	Time-of-Flight (UPLC-ESI-TOF) metabolome data	1613:1657	Time-of-Flight (UPLC-ESI-TOF) metabolome data	1613:1657	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	1	10	theme	Amazonian	192:200	arg1	plants					202:207	Amazonian plants	192:207	Amazonian plants	192:207	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca is a tea produced through decoction of Amazonian plants.					
32171895	6	11	from	plants	806:811	arg1	extracts					781:788	the extracts	777:788	the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis)	777:857	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	11	from	plants	806:811	arg1	fractions					864:872	its fractions	860:872	its fractions	860:872	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	11	from	plants	806:811	arg1	Psychotria					839:848	Psychotria	839:848	Psychotria	839:848	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	11	from	plants	806:811	arg1	beverage					767:774	the Ayahuasca beverage	753:774	the Ayahuasca beverage	753:774	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	11	from	plants	806:811	arg1	alkaloids					887:895	its main alkaloids	878:895	its main alkaloids	878:895	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	11	from	plants	806:811	arg1	caapi					829:833	caapi	829:833	caapi	829:833	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	7	12	theme	crude	1022:1026	arg1	extracts					1028:1035	crude extracts	1022:1035	crude extracts	1022:1035	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	11	13	theme	house	1929:1933	arg1	database					1935:1942	house database	1929:1942	house database	1929:1942	The metabolites were dereplicated using the Dictionary of Natural Products and an in house database.					
32171895	16	14	theme	2419	2489:2492	arg1	compounds					2494:2502	2419 compounds	2489:2502	2419 compounds	2489:2502	Additionally, a total of 2419 compounds were detected in both ionisation modes.					
32171895	7	15	theme	Ayahuasca	1011:1019	arg1	neuroprotection					1091:1105	neuroprotection	1091:1105	neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1091:1195	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	15	theme	Ayahuasca	1011:1019	arg1	METHODS					983:989	METHODS	983:989	METHODS	983:989	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	15	theme	Ayahuasca	1011:1019	arg1	MATERIAL					970:977	MATERIAL	970:977	MATERIAL	970:977	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	17	16	theme	neuroprotective	2679:2693	arg1	profile					2695:2701	the most efficacious neuroprotective profile	2658:2701	the most efficacious neuroprotective profile at either time (T1 and T2)	2658:2728	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	14	17	theme	alkaloid	2139:2146	arg1	fractions					2148:2156	alkaloid fractions	2139:2156	alkaloid fractions	2139:2156	The crude extracts, alkaloid fractions and HRE demonstrated remarkable neuroprotective effect at T2 while the hydroalcoholic fractions demonstrated this neuroprotective effect at T1 and T2.					
32171895	8	18	theme	matrix	1309:1314	arg1	plants					1316:1321	Ayahuasca matrix plants	1299:1321	Ayahuasca matrix plants	1299:1321	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT), were also isolated and evaluated.					
32171895	22	19	theme	potential	3534:3542	arg1	applicability					3544:3556	potential applicability	3534:3556	potential applicability for PD treatment	3534:3573	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	10	20	theme	multivariate	1698:1709	arg1	MSA					1733:1735	MSA	1733:1735	MSA	1733:1735	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	20	theme	multivariate	1698:1709	arg1	analyses					1723:1730	multivariate statistical analyses	1698:1730	multivariate statistical analyses (MSA)	1698:1736	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	17	21	theme	neuroprotective	2562:2576	arg1	action					2578:2583	potent neuroprotective action	2555:2583	potent neuroprotective action	2555:2583	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	10	22	dep	treated	1663:1669	arg1	analysis					1824:1831	orthogonal partial least squares discriminant analysis	1778:1831	orthogonal partial least squares discriminant analysis (OPLS-DA)	1778:1841	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	22	dep	treated	1663:1669	arg1	analysis					1759:1766	principal component analysis	1739:1766	principal component analysis (PCA)	1739:1772	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	22	dep	treated	1663:1669	arg1	PCA					1769:1771	PCA	1769:1771	PCA	1769:1771	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	22	dep	treated	1663:1669	arg1	OPLS-DA					1834:1840	OPLS-DA	1834:1840	OPLS-DA	1834:1840	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	17	23	dep	time	2713:2716	arg1	T2					2726:2727	T2	2726:2727	T2	2726:2727	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	17	23	dep	time	2713:2716	arg1	T1					2719:2720	T1	2719:2720	T1	2719:2720	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	22	24	theme	base	3517:3520	arg1	plants					3522:3527	its base plants	3513:3527	its base plants	3513:3527	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	15	25	theme	monoterpene	2376:2386	arg1	MIAs					2406:2409	MIAs	2406:2409	MIAs	2406:2409	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	15	25	theme	monoterpene	2376:2386	arg1	alkaloids					2395:2403	monoterpene indole alkaloids	2376:2403	monoterpene indole alkaloids (MIAs)	2376:2410	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	20	26	theme	enhanced	3315:3322	arg1	effects					3340:3346	enhanced neuroprotective effects	3315:3346	enhanced neuroprotective effects	3315:3346	Intriguingly, the hydroalcoholic fractions exhibited enhanced neuroprotective effects when compared to other samples and isolated alkaloids.					
32171895	16	27	theme	ionisation	2526:2535	arg1	modes					2537:2541	both ionisation modes	2521:2541	both ionisation modes	2521:2541	Additionally, a total of 2419 compounds were detected in both ionisation modes.					
32171895	19	28	theme	neuroprotection	3138:3152	arg1	profile					3154:3160	the most efficacious neuroprotection profile	3117:3160	the most efficacious neuroprotection profile	3117:3160	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	6	29	theme	PD	960:961	arg1	model					963:967	an in vitro PD model	948:967	an in vitro PD model	948:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	30	theme	in	951:952	arg1	model					963:967	an in vitro PD model	948:967	an in vitro PD model	948:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	12	31	theme	main	1949:1952	arg1	alkaloids					1954:1962	The main alkaloids	1945:1962	The main alkaloids	1945:1962	The main alkaloids were also quantified by UPLC-MS/MS.					
32171895	19	32	theme	neuronal	3072:3079	arg1	proliferation					3086:3098	neuronal cell proliferation	3072:3098	neuronal cell proliferation	3072:3098	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	1	33	theme	ETHNOPHARMACOLOGY	115:131	arg1	Ayahuasca					143:151	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca	115:151	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca	115:151	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca is a tea produced through decoction of Amazonian plants.					
32171895	1	33	theme	ETHNOPHARMACOLOGY	115:131	arg1	tea					158:160	a tea	156:160	a tea produced through decoction of Amazonian plants	156:207	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca is a tea produced through decoction of Amazonian plants.					
32171895	19	34	theme	6-OHDA-induced	3237:3250	arg1	toxicity					3252:3259	6-OHDA-induced toxicity	3237:3259	6-OHDA-induced toxicity	3237:3259	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	15	35	theme	Several	2309:2315	arg1	compounds					2317:2325	Several compounds	2309:2325	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs)	2309:2410	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	11	36	theme	Products	1910:1917	arg1	Dictionary					1888:1897	the Dictionary	1884:1897	the Dictionary of Natural Products	1884:1917	The metabolites were dereplicated using the Dictionary of Natural Products and an in house database.					
32171895	21	37	theme	approach	3460:3467	arg1	significance					3433:3444	the significance	3429:3444	the significance of a holistic approach	3429:3467	This finding corroborates the significance of a holistic approach.					
32171895	6	38	theme	neuroblastoma	925:937	arg1	cells					939:943	SH-SY5Y neuroblastoma cells	917:943	SH-SY5Y neuroblastoma cells	917:943	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	4	39	theme	scientific	453:462	arg1	evidence					464:471	some scientific evidence	448:471	some scientific evidence	448:471	Besides, some scientific evidence suggests it may be applicable to Parkinson's disease (PD) treatment.					
32171895	3	40	theme	neurological	417:428	arg1	illness					430:436	neurological illness	417:436	neurological illness	417:436	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	0	41	theme	untargeted	43:52	arg1	metabolomics					54:65	untargeted metabolomics	43:65	untargeted metabolomics	43:65	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.					
32171895	19	42	theme	efficacious	3126:3136	arg1	profile					3154:3160	the most efficacious neuroprotection profile	3117:3160	the most efficacious neuroprotection profile	3117:3160	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	0	43	theme	Neuroprotective	0:14	arg1	potential					16:24	Neuroprotective potential	0:24	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.	0:113	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.					
32171895	3	44	used	used	355:358	arg2	it					335:336	it	335:336	it	335:336	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	18	45	with	correlation	2924:2934	arg1	T1					2941:2942	T1	2941:2942	T1	2941:2942	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	18	45	with	correlation	2924:2934	arg1	T2					2948:2949	T2	2948:2949	T2	2948:2949	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	6	46	dep	extracts	781:788	arg1	extracts					781:788	the extracts	777:788	the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis)	777:857	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	46	dep	extracts	781:788	arg1	Psychotria					839:848	Psychotria	839:848	Psychotria	839:848	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	46	dep	extracts	781:788	arg1	caapi					829:833	caapi	829:833	caapi	829:833	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	47	from	viability	904:912	arg1	model					963:967	an in vitro PD model	948:967	an in vitro PD model	948:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	48	dep	aimed	659:663	arg1	AIM					631:633	AIM	631:633	AIM OF THE STUDY	631:646	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	0	49	dep	potential	16:24	arg1	applicability					77:89	applicability	77:89	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.	0:113	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.					
32171895	10	50	theme	Liquid	1552:1557	arg1	Chromatography					1559:1572	Ultra-Performance Liquid Chromatography	1534:1572	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data	1534:1657	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	8	51	from	N	1342:1342	arg1	alkaloids					1284:1292	The main alkaloids	1275:1292	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT),	1275:1370	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT), were also isolated and evaluated.					
32171895	18	52	theme	in	2889:2890	arg1	assay					2898:2902	in vitro assay	2889:2902	in vitro assay	2889:2902	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	9	53	theme	metabolomics	1420:1431	arg1	approach					1433:1440	An untargeted metabolomics approach	1406:1440	An untargeted metabolomics approach	1406:1440	An untargeted metabolomics approach was developed to explore the chemical composition of samples with neuroprotective activity.					
32171895	20	54	theme	other	3365:3369	arg1	samples					3371:3377	other samples	3365:3377	other samples	3365:3377	Intriguingly, the hydroalcoholic fractions exhibited enhanced neuroprotective effects when compared to other samples and isolated alkaloids.					
32171895	7	55	theme	MTT	1220:1222	arg1	assay					1224:1228	the MTT assay	1216:1228	the MTT assay	1216:1228	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	10	56	theme	component	1749:1757	arg1	PCA					1769:1771	PCA	1769:1771	PCA	1769:1771	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	56	theme	component	1749:1757	arg1	analysis					1759:1766	principal component analysis	1739:1766	principal component analysis (PCA)	1739:1772	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	14	57	theme	crude	2123:2127	arg1	extracts					2129:2136	The crude extracts	2119:2136	The crude extracts	2119:2136	The crude extracts, alkaloid fractions and HRE demonstrated remarkable neuroprotective effect at T2 while the hydroalcoholic fractions demonstrated this neuroprotective effect at T1 and T2.					
32171895	7	58	theme	viridis	1071:1077	arg1	extracts					1028:1035	crude extracts	1022:1035	crude extracts	1022:1035	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	58	theme	viridis	1071:1077	arg1	fractions					1042:1050	fractions	1042:1050	fractions	1042:1050	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	58	theme	viridis	1071:1077	arg1	Ayahuasca					1011:1019	Ayahuasca	1011:1019	Ayahuasca	1011:1019	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	18	59	theme	positive	2915:2922	arg1	correlation					2924:2934	a positive correlation	2913:2934	a positive correlation with T1 and T2	2913:2949	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	17	60	theme	efficacious	2667:2677	arg1	profile					2695:2701	the most efficacious neuroprotective profile	2658:2701	the most efficacious neuroprotective profile at either time (T1 and T2)	2658:2728	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	3	61	theme	wide	383:386	arg1	range					388:392	a wide range	381:392	a wide range of diseases, including neurological illness	381:436	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	3	61	theme	wide	383:386	arg1	illness					430:436	neurological illness	417:436	neurological illness	417:436	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	10	62	theme	Electrospray	1585:1596	arg1	Ionisation					1598:1607	Electrospray Ionisation	1585:1607	Electrospray Ionisation	1585:1607	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	0	63	theme	metabolomics	54:65	arg1	potential					16:24	Neuroprotective potential	0:24	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.	0:113	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.					
32171895	13	64	theme	cell	2097:2100	arg1	viability					2102:2110	cell viability	2097:2110	cell viability	2097:2110	RESULTS The samples did not cause cytotoxicity in vitro and three of samples intensely increased cell viability at T1.					
32171895	6	65	theme	matrix	799:804	arg1	plants					806:811	its matrix plants	795:811	its matrix plants	795:811	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	4	66	theme	disease	518:524	arg1	treatment					531:539	Parkinson's disease (PD) treatment	506:539	Parkinson's disease (PD) treatment	506:539	Besides, some scientific evidence suggests it may be applicable to Parkinson's disease (PD) treatment.					
32171895	21	67	theme	holistic	3451:3458	arg1	approach					3460:3467	a holistic approach	3449:3467	a holistic approach	3449:3467	This finding corroborates the significance of a holistic approach.					
32171895	8	68	theme	Ayahuasca	1299:1307	arg1	plants					1316:1321	Ayahuasca matrix plants	1299:1321	Ayahuasca matrix plants	1299:1321	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT), were also isolated and evaluated.					
32171895	6	69	theme	untargeted	675:684	arg1	approach					699:706	an untargeted metabolomics approach	672:706	an untargeted metabolomics approach	672:706	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	15	70	from	classes	2342:2348	arg1	compounds					2317:2325	Several compounds	2309:2325	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs)	2309:2410	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	7	71	theme	-induced	1164:1171	arg1	model					1191:1195	a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1136:1195	a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1136:1195	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	10	72	theme	least	1797:1801	arg1	analysis					1824:1831	orthogonal partial least squares discriminant analysis	1778:1831	orthogonal partial least squares discriminant analysis (OPLS-DA)	1778:1841	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	72	theme	least	1797:1801	arg1	OPLS-DA					1834:1840	OPLS-DA	1834:1840	OPLS-DA	1834:1840	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	9	73	theme	samples	1495:1501	arg1	composition					1480:1490	the chemical composition	1467:1490	the chemical composition of samples with neuroprotective activity	1467:1531	An untargeted metabolomics approach was developed to explore the chemical composition of samples with neuroprotective activity.					
32171895	22	74	from	drugs	3631:3635	arg1	progression					3594:3604	its progression	3590:3604	its progression differently from current drugs to treat PD	3590:3647	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	19	75	dep	CONCLUSION	3012:3021	arg1	displayed					3107:3115	displayed	3107:3115	displayed the most efficacious neuroprotection profile	3107:3160	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	19	75	dep	CONCLUSION	3012:3021	arg1	stimulated					3061:3070	stimulated	3061:3070	stimulated neuronal cell proliferation	3061:3098	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	13	76	dep	RESULTS	2000:2006	arg1	cause					2028:2032	cause	2028:2032	did not cause cytotoxicity in vitro	2020:2054	RESULTS The samples did not cause cytotoxicity in vitro and three of samples intensely increased cell viability at T1.					
32171895	13	76	dep	RESULTS	2000:2006	arg1	increased					2087:2095	increased	2087:2095	increased cell viability at T1	2087:2116	RESULTS The samples did not cause cytotoxicity in vitro and three of samples intensely increased cell viability at T1.					
32171895	10	77	theme	orthogonal	1778:1787	arg1	analysis					1824:1831	orthogonal partial least squares discriminant analysis	1778:1831	orthogonal partial least squares discriminant analysis (OPLS-DA)	1778:1841	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	77	theme	orthogonal	1778:1787	arg1	OPLS-DA					1834:1840	OPLS-DA	1834:1840	OPLS-DA	1834:1840	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	11	78	theme	Natural	1902:1908	arg1	Products					1910:1917	Natural Products	1902:1917	Natural Products	1902:1917	The metabolites were dereplicated using the Dictionary of Natural Products and an in house database.					
32171895	18	79	theme	sufficient	2850:2859	arg1	activity					2877:2884	sufficient neuroprotective activity	2850:2884	sufficient neuroprotective activity by in vitro assay	2850:2902	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	9	80	theme	neuroprotective	1508:1522	arg1	activity					1524:1531	neuroprotective activity	1508:1531	neuroprotective activity	1508:1531	An untargeted metabolomics approach was developed to explore the chemical composition of samples with neuroprotective activity.					
32171895	18	81	theme	VIP	2806:2808	arg1	dataset					2797:2803	the dataset	2793:2803	the dataset (VIP value > 1)	2793:2819	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	18	81	theme	VIP	2806:2808	arg1	value > 1					2810:2818	VIP value > 1	2806:2818	VIP value > 1	2806:2818	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	7	82	theme	extracts	1028:1035	arg1	neuroprotection					1091:1105	neuroprotection	1091:1105	neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1091:1195	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	82	theme	extracts	1028:1035	arg1	METHODS					983:989	METHODS	983:989	METHODS	983:989	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	82	theme	extracts	1028:1035	arg1	MATERIAL					970:977	MATERIAL	970:977	MATERIAL	970:977	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	6	83	theme	Ayahuasca	757:765	arg1	alkaloids					887:895	its main alkaloids	878:895	its main alkaloids	878:895	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	83	theme	Ayahuasca	757:765	arg1	beverage					767:774	the Ayahuasca beverage	753:774	the Ayahuasca beverage	753:774	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	83	theme	Ayahuasca	757:765	arg1	extracts					781:788	the extracts	777:788	the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis)	777:857	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	83	theme	Ayahuasca	757:765	arg1	fractions					864:872	its fractions	860:872	its fractions	860:872	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	18	84	theme	MSA	2999:3001	arg1	results					3003:3009	the MSA results	2995:3009	the MSA results	2995:3009	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	16	85	located	detected	2509:2516	arg2	total					2480:2484	a total	2478:2484	a total of 2419 compounds	2478:2502	Additionally, a total of 2419 compounds were detected in both ionisation modes.					
32171895	16	85	located	detected	2509:2516	arg1	modes					2537:2541	both ionisation modes	2521:2541	both ionisation modes	2521:2541	Additionally, a total of 2419 compounds were detected in both ionisation modes.					
32171895	10	86	theme	discriminant	1811:1822	arg1	analysis					1824:1831	orthogonal partial least squares discriminant analysis	1778:1831	orthogonal partial least squares discriminant analysis (OPLS-DA)	1778:1841	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	86	theme	discriminant	1811:1822	arg1	OPLS-DA					1834:1840	OPLS-DA	1834:1840	OPLS-DA	1834:1840	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	5	87	theme	potential	599:607	arg1	role					609:612	its potential role	595:612	its potential role in this disease	595:628	Thus, Ayahuasca deserves in depth studies to clarify its potential role in this disease.					
32171895	16	88	theme	compounds	2494:2502	arg1	total					2480:2484	a total	2478:2484	a total of 2419 compounds	2478:2502	Additionally, a total of 2419 compounds were detected in both ionisation modes.					
32171895	1	89	theme	plants	202:207	arg1	decoction					179:187	decoction	179:187	decoction of Amazonian plants	179:207	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca is a tea produced through decoction of Amazonian plants.					
32171895	6	90	theme	SH-SY5Y	917:923	arg1	cells					939:943	SH-SY5Y neuroblastoma cells	917:943	SH-SY5Y neuroblastoma cells	917:943	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	17	91	from	time	2713:2716	arg1	profile					2695:2701	the most efficacious neuroprotective profile	2658:2701	the most efficacious neuroprotective profile at either time (T1 and T2)	2658:2728	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	6	92	theme	neuroprotective	724:738	arg1	potential					740:748	the neuroprotective potential	720:748	the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model	720:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	19	93	theme	active	3046:3051	arg1	samples					3053:3059	the active samples	3042:3059	the active samples	3042:3059	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	10	94	theme	statistical	1711:1721	arg1	MSA					1733:1735	MSA	1733:1735	MSA	1733:1735	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	94	theme	statistical	1711:1721	arg1	analyses					1723:1730	multivariate statistical analyses	1698:1730	multivariate statistical analyses (MSA)	1698:1736	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	17	95	theme	potent	2555:2560	arg1	action					2578:2583	potent neuroprotective action	2555:2583	potent neuroprotective action	2555:2583	HRE showed potent neuroprotective action at 72 h, but it was not among the metabolites positively correlated with the most efficacious neuroprotective profile at either time (T1 and T2).					
32171895	18	96	dep	activity	2877:2884	arg1	neither					2905:2911	neither	2905:2911	neither	2905:2911	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	18	96	dep	activity	2877:2884	arg1	profile					2967:2973	neuroprotective profile	2951:2973	neuroprotective profile	2951:2973	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	1	97	theme	RELEVANCE	133:141	arg1	Ayahuasca					143:151	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca	115:151	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca	115:151	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca is a tea produced through decoction of Amazonian plants.					
32171895	1	97	theme	RELEVANCE	133:141	arg1	tea					158:160	a tea	156:160	a tea produced through decoction of Amazonian plants	156:207	ETHNOPHARMACOLOGY RELEVANCE Ayahuasca is a tea produced through decoction of Amazonian plants.					
32171895	6	98	theme	main	882:885	arg1	alkaloids					887:895	its main alkaloids	878:895	its main alkaloids	878:895	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	98	theme	main	882:885	arg1	beverage					767:774	the Ayahuasca beverage	753:774	the Ayahuasca beverage	753:774	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	14	99	theme	hydroalcoholic	2229:2242	arg1	fractions					2244:2252	the hydroalcoholic fractions	2225:2252	the hydroalcoholic fractions	2225:2252	The crude extracts, alkaloid fractions and HRE demonstrated remarkable neuroprotective effect at T2 while the hydroalcoholic fractions demonstrated this neuroprotective effect at T1 and T2.					
32171895	19	100	theme	neuronal	3184:3191	arg1	damage					3193:3198	neuronal damage	3184:3198	neuronal damage	3184:3198	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	0	101	dep	Ayahuasca	29:37	arg1	analyses					67:74	analyses	67:74	analyses	67:74	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.					
32171895	2	102	theme	America	271:277	arg1	people					255:260	indigenous people	244:260	indigenous people of South America	244:277	It has been used for centuries by indigenous people of South America.					
32171895	15	103	theme	indole	2388:2393	arg1	MIAs					2406:2409	MIAs	2406:2409	MIAs	2406:2409	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	15	103	theme	indole	2388:2393	arg1	alkaloids					2395:2403	monoterpene indole alkaloids	2376:2403	monoterpene indole alkaloids (MIAs)	2376:2410	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	19	104	theme	lower	3027:3031	arg1	doses					3033:3037	The lower doses	3023:3037	The lower doses of the active samples	3023:3059	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	8	105	from	harmine	1324:1330	arg1	alkaloids					1284:1292	The main alkaloids	1275:1292	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT),	1275:1370	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT), were also isolated and evaluated.					
32171895	14	106	theme	remarkable	2179:2188	arg1	effect					2206:2211	remarkable neuroprotective effect	2179:2211	remarkable neuroprotective effect	2179:2211	The crude extracts, alkaloid fractions and HRE demonstrated remarkable neuroprotective effect at T2 while the hydroalcoholic fractions demonstrated this neuroprotective effect at T1 and T2.					
32171895	15	107	dep	revealed	2417:2424	arg1	correlated					2426:2435	correlated	2426:2435	were revealed correlated with this property by MSA	2412:2461	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	20	108	theme	neuroprotective	3324:3338	arg1	effects					3340:3346	enhanced neuroprotective effects	3315:3346	enhanced neuroprotective effects	3315:3346	Intriguingly, the hydroalcoholic fractions exhibited enhanced neuroprotective effects when compared to other samples and isolated alkaloids.					
32171895	19	109	theme	cell	3214:3217	arg1	viability					3219:3227	cell viability	3214:3227	cell viability	3214:3227	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	8	110	from	plants	1316:1321	arg1	alkaloids					1284:1292	The main alkaloids	1275:1292	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT),	1275:1370	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT), were also isolated and evaluated.					
32171895	18	111	theme	neuroprotective	2951:2965	arg1	profile					2967:2973	neuroprotective profile	2951:2973	neuroprotective profile	2951:2973	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	2	112	used	used	222:225	arg2	It					210:211	It	210:211	It	210:211	It has been used for centuries by indigenous people of South America.					
32171895	14	113	theme	neuroprotective	2190:2204	arg1	effect					2206:2211	remarkable neuroprotective effect	2179:2211	remarkable neuroprotective effect	2179:2211	The crude extracts, alkaloid fractions and HRE demonstrated remarkable neuroprotective effect at T2 while the hydroalcoholic fractions demonstrated this neuroprotective effect at T1 and T2.					
32171895	15	114	theme	different	2332:2340	arg1	β-carbolines					2359:2370	β-carbolines	2359:2370	β-carbolines	2359:2370	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	15	114	theme	different	2332:2340	arg1	alkaloids					2395:2403	monoterpene indole alkaloids	2376:2403	monoterpene indole alkaloids (MIAs)	2376:2410	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	15	114	theme	different	2332:2340	arg1	classes					2342:2348	different classes	2332:2348	different classes	2332:2348	Several compounds from different classes, such as β-carbolines and monoterpene indole alkaloids (MIAs) were revealed correlated with this property by MSA.					
32171895	5	115	theme	depth	570:574	arg1	studies					576:582	depth studies	570:582	depth studies	570:582	Thus, Ayahuasca deserves in depth studies to clarify its potential role in this disease.					
32171895	20	116	theme	hydroalcoholic	3280:3293	arg1	fractions					3295:3303	the hydroalcoholic fractions	3276:3303	the hydroalcoholic fractions	3276:3303	Intriguingly, the hydroalcoholic fractions exhibited enhanced neuroprotective effects when compared to other samples and isolated alkaloids.					
32171895	6	117	theme	cells	939:943	arg1	viability					904:912	the viability	900:912	the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model	900:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	18	118	dep	in	2889:2890	arg1	vitro					2892:2896	vitro	2892:2896	vitro	2892:2896	Furthermore, DMT was statistically important to differentiate the dataset (VIP value > 1), although it did not exhibit sufficient neuroprotective activity by in vitro assay, neither a positive correlation with T1 and T2 neuroprotective profile, which corroborated the MSA results.					
32171895	22	119	contain	have	3529:3532	arg2	applicability					3544:3556	potential applicability	3534:3556	potential applicability for PD treatment	3534:3573	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	22	119	contain	have	3529:3532	arg1	plants					3522:3527	its base plants	3513:3527	its base plants	3513:3527	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	22	119	contain	have	3529:3532	arg1	Ayahuasca					3499:3507	Ayahuasca	3499:3507	Ayahuasca	3499:3507	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	19	120	theme	samples	3053:3059	arg1	doses					3033:3037	The lower doses	3023:3037	The lower doses of the active samples	3023:3059	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	19	121	theme	cell	3081:3084	arg1	proliferation					3086:3098	neuronal cell proliferation	3072:3098	neuronal cell proliferation	3072:3098	CONCLUSION The lower doses of the active samples stimulated neuronal cell proliferation and/or displayed the most efficacious neuroprotection profile, namely by preventing neuronal damage and improving cell viability against 6-OHDA-induced toxicity.					
32171895	22	122	theme	PD	3562:3563	arg1	treatment					3565:3573	PD treatment	3562:3573	PD treatment	3562:3573	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	6	123	dep	in	951:952	arg1	vitro					954:958	vitro	954:958	vitro	954:958	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	6	124	theme	STUDY	642:646	arg1	AIM					631:633	AIM	631:633	AIM OF THE STUDY	631:646	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	0	125	theme	Ayahuasca	29:37	arg1	potential					16:24	Neuroprotective potential	0:24	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.	0:113	Neuroprotective potential of Ayahuasca and untargeted metabolomics analyses: applicability to Parkinson's disease.					
32171895	5	126	from	role	609:612	arg1	disease					622:628	this disease	617:628	this disease	617:628	Thus, Ayahuasca deserves in depth studies to clarify its potential role in this disease.					
32171895	9	127	theme	untargeted	1409:1418	arg1	approach					1433:1440	An untargeted metabolomics approach	1406:1440	An untargeted metabolomics approach	1406:1440	An untargeted metabolomics approach was developed to explore the chemical composition of samples with neuroprotective activity.					
32171895	2	128	theme	indigenous	244:253	arg1	people					255:260	indigenous people	244:260	indigenous people of South America	244:277	It has been used for centuries by indigenous people of South America.					
32171895	6	129	from	potential	740:748	arg1	viability					904:912	the viability	900:912	the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model	900:967	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	10	130	theme	Ultra-Performance	1534:1550	arg1	Chromatography					1559:1572	Ultra-Performance Liquid Chromatography	1534:1572	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data	1534:1657	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	7	131	theme	P.	1068:1069	arg1	viridis					1071:1077	P. viridis	1068:1077	P. viridis	1068:1077	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	3	132	theme	diseases	397:404	arg1	range					388:392	a wide range	381:392	a wide range of diseases, including neurological illness	381:436	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	3	132	theme	diseases	397:404	arg1	illness					430:436	neurological illness	417:436	neurological illness	417:436	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	10	133	theme	principal	1739:1747	arg1	PCA					1769:1771	PCA	1769:1771	PCA	1769:1771	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	133	theme	principal	1739:1747	arg1	analysis					1759:1766	principal component analysis	1739:1766	principal component analysis (PCA)	1739:1772	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	7	134	theme	caapi	1058:1062	arg1	extracts					1028:1035	crude extracts	1022:1035	crude extracts	1022:1035	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	134	theme	caapi	1058:1062	arg1	fractions					1042:1050	fractions	1042:1050	fractions	1042:1050	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	134	theme	caapi	1058:1062	arg1	Ayahuasca					1011:1019	Ayahuasca	1011:1019	Ayahuasca	1011:1019	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	3	135	theme	range	388:392	arg1	treatment					368:376	the treatment	364:376	the treatment of a wide range of diseases, including neurological illness	364:436	The beverage is considered to be an ethnomedicine, and it is traditionally used for the treatment of a wide range of diseases, including neurological illness.					
32171895	6	136	dep	caapi	829:833	arg1	viridis					850:856	viridis	850:856	viridis	850:856	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	7	137	dep	evaluated	1203:1211	arg1	T2					1270:1271	T2	1270:1271	T2	1270:1271	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	137	dep	evaluated	1203:1211	arg1	T1					1256:1257	T1	1256:1257	T1	1256:1257	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	137	dep	evaluated	1203:1211	arg1	72 h					1264:1267	72 h	1264:1267	72 h	1264:1267	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	137	dep	evaluated	1203:1211	arg1	48 h					1250:1253	48 h	1250:1253	48 h	1250:1253	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	7	138	theme	neurodegeneration	1173:1189	arg1	model					1191:1195	a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1136:1195	a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model	1136:1195	MATERIAL AND METHODS The cytotoxicity of Ayahuasca, crude extracts, and fractions of B. caapi and P. viridis, as well as neuroprotection promoted by these samples in a 6-hydroxydopamine (6-OHDA)-induced neurodegeneration model, were evaluated by the MTT assay at two time-points: 48 h (T1) and 72 h (T2).					
32171895	8	139	theme	main	1279:1282	arg1	alkaloids					1284:1292	The main alkaloids	1275:1292	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT),	1275:1370	The main alkaloids from Ayahuasca matrix plants, harmine (HRE) and N,N-dimethyltryptamine (DMT), were also isolated and evaluated.					
32171895	6	140	theme	metabolomics	686:697	arg1	approach					699:706	an untargeted metabolomics approach	672:706	an untargeted metabolomics approach	672:706	AIM OF THE STUDY This study aimed to use an untargeted metabolomics approach to evaluate the neuroprotective potential of the Ayahuasca beverage, the extracts from its matrix plants (Banisteriopsis caapi and Psychotria viridis), its fractions and its main alkaloids on the viability of SH-SY5Y neuroblastoma cells in an in vitro PD model.					
32171895	9	141	theme	chemical	1471:1478	arg1	composition					1480:1490	the chemical composition	1467:1490	the chemical composition of samples with neuroprotective activity	1467:1531	An untargeted metabolomics approach was developed to explore the chemical composition of samples with neuroprotective activity.					
32171895	10	142	theme	squares	1803:1809	arg1	analysis					1824:1831	orthogonal partial least squares discriminant analysis	1778:1831	orthogonal partial least squares discriminant analysis (OPLS-DA)	1778:1841	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	10	142	theme	squares	1803:1809	arg1	OPLS-DA					1834:1840	OPLS-DA	1834:1840	OPLS-DA	1834:1840	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	22	143	theme	current	3623:3629	arg1	drugs					3631:3635	current drugs	3623:3635	current drugs	3623:3635	The results demonstrate that Ayahuasca and its base plants have potential applicability for PD treatment and to prevent its progression differently from current drugs to treat PD.					
32171895	10	144	theme	Time-of-Flight	1613:1626	arg1	data					1654:1657	Time-of-Flight (UPLC-ESI-TOF) metabolome data	1613:1657	Time-of-Flight (UPLC-ESI-TOF) metabolome data	1613:1657	Ultra-Performance Liquid Chromatography coupled to Electrospray Ionisation and Time-of-Flight (UPLC-ESI-TOF) metabolome data was treated and further analysed using multivariate statistical analyses (MSA): principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).					
32171895	13	145	theme	samples	2069:2075	arg1	three					2060:2064	three	2060:2064	three	2060:2064	RESULTS The samples did not cause cytotoxicity in vitro and three of samples intensely increased cell viability at T1.					
32171895	13	145	theme	samples	2069:2075	arg1	samples					2069:2075	samples	2069:2075	samples	2069:2075	RESULTS The samples did not cause cytotoxicity in vitro and three of samples intensely increased cell viability at T1.					
33565641	11	0	theme	direct-expanded	2047:2061	arg1	products					2063:2070	direct-expanded products	2047:2070	direct-expanded products	2047:2070	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	2	1	theme	temperature	319:329	arg1	effects					301:307	The effects	297:307	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics	297:443	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	9	2	theme	significant	1566:1576	arg1	differences					1578:1588	The significant differences	1562:1588	The significant differences in the properties of flours from the three varieties of lentils	1562:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	3	3	contain	had	531:533	arg1	lentils					523:529	lentils	523:529	lentils	523:529	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	3	contain	had	531:533	arg2	p < 0.05					560:567	p < 0.05	560:567	p < 0.05	560:567	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	3	contain	had	531:533	arg1	varieties					510:518	The three varieties	500:518	The three varieties of lentils	500:529	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	3	contain	had	531:533	arg2	differences					547:557	significant differences	535:557	significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature	535:769	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	6	4	theme	melting	1257:1263	arg1	temperature					1265:1275	melting temperature	1257:1275	melting temperature	1257:1275	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	12	5	theme	thermal	2159:2165	arg1	properties					2167:2176	thermal properties	2159:2176	thermal properties	2159:2176	The data prove the importance of understanding the chemical composition, pasting, and thermal properties to select the appropriate varieties for extrusion processing.					
33565641	12	6	theme	extrusion	2218:2226	arg1	processing					2228:2237	extrusion processing	2218:2237	extrusion processing	2218:2237	The data prove the importance of understanding the chemical composition, pasting, and thermal properties to select the appropriate varieties for extrusion processing.					
33565641	7	7	dep	3.0	1349:1351	arg1	to					1346:1347	to	1346:1347	to	1346:1347	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	7	8	theme	extrusion	1427:1435	arg1	conditions					1437:1446	the extrusion conditions	1423:1446	the extrusion conditions studied	1423:1454	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	0	9	theme	expansion	126:134	arg1	characteristics					136:150	their expansion characteristics	120:150	their expansion characteristics	120:150	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	6	10	theme	lowest	1230:1235	arg1	content					1245:1251	protein content	1237:1251	protein content	1237:1251	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	3	11	dep	had	531:533	arg1	119.7 °C					781:788	119.7 °C	781:788	119.7 °C	781:788	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	2	12	theme	twin-screw	479:488	arg1	extruder					490:497	a corotating twin-screw extruder	466:497	a corotating twin-screw extruder	466:497	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	9	13	theme	flours	1611:1616	arg1	properties					1597:1606	the properties	1593:1606	the properties of flours from the three varieties of lentils	1593:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	0	14	theme	significant	94:104	arg1	variations					106:115	significant variations	94:115	significant variations in their expansion characteristics	94:150	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	4	15	theme	extrudate	897:905	arg1	properties					907:916	extrudate properties	897:916	extrudate properties	897:916	The lentil variety, barrel temperature, and screw speed significantly impacted the process responses and extrudate properties.					
33565641	3	16	theme	fat	634:636	arg1	content					638:644	fat content	634:644	fat content	634:644	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	7	17	dep	exhibited	1304:1312	arg1	3.0					1349:1351	3.0	1349:1351	3.0	1349:1351	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	1	18	theme	characteristics	209:223	arg1	properties					174:183	The properties	170:183	The properties	170:183	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	6	19	theme	Richlea	1064:1070	arg1	variety					1072:1078	Richlea variety	1064:1078	Richlea variety	1064:1078	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	20	contain	had	1080:1082	arg2	ratio					1106:1110	the highest expansion ratio	1084:1110	the highest expansion ratio (3.6)	1084:1116	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	20	contain	had	1080:1082	arg1	variety					1072:1078	Richlea variety	1064:1078	Richlea variety	1064:1078	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	20	contain	had	1080:1082	arg2	%					1162:1162	45.4%	1158:1162	45.4%	1158:1162	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	20	contain	had	1080:1082	arg2	index					1151:1155	the highest water solubility index	1122:1155	the highest water solubility index (45.4%)	1122:1163	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	20	contain	had	1080:1082	arg2	3.6					1113:1115	3.6	1113:1115	3.6	1113:1115	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	7	21	theme	expansion	1325:1333	arg1	ratio					1335:1339	the lowest expansion ratio	1314:1339	the lowest expansion ratio	1314:1339	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	9	22	theme	lentils	1646:1652	arg1	lentils					1646:1652	lentils	1646:1652	lentils	1646:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	9	22	theme	lentils	1646:1652	arg1	varieties					1633:1641	the three varieties	1623:1641	the three varieties of lentils	1623:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	3	23	theme	%	650:650	arg1	%					658:658	1.3% to 1.9%	647:658	1.3% to 1.9%	647:658	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	23	theme	%	650:650	arg1	starch					579:584	their starch	573:584	their starch (48.7% to 50.9%)	573:601	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	24	theme	gelatinization	662:675	arg1	74.6 °C					698:704	74.6 °C	698:704	74.6 °C	698:704	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	24	theme	gelatinization	662:675	arg1	temperature					677:687	gelatinization temperature	662:687	gelatinization temperature (71.7 to 74.6 °C)	662:705	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	9	25	from	differences	1578:1588	arg1	properties					1597:1606	the properties	1593:1606	the properties of flours from the three varieties of lentils	1593:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	6	26	theme	highest	1126:1132	arg1	index					1151:1155	the highest water solubility index	1122:1155	the highest water solubility index (45.4%)	1122:1163	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	26	theme	highest	1126:1132	arg1	%					1162:1162	45.4%	1158:1162	45.4%	1158:1162	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	8	27	theme	Richlea	1457:1463	arg1	variety					1465:1471	Richlea variety	1457:1471	Richlea variety	1457:1471	Richlea variety was the most suitable for making direct-expanded extrudates among the varieties studied.					
33565641	8	27	theme	Richlea	1457:1463	arg1	suitable					1486:1493	suitable	1486:1493	suitable	1486:1493	Richlea variety was the most suitable for making direct-expanded extrudates among the varieties studied.					
33565641	2	28	theme	process	397:403	arg1	responses					405:413	process responses	397:413	process responses	397:413	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	3	29	dep	%	626:626	arg1	to					619:620	to	619:620	to	619:620	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	4	30	dep	speed	842:846	arg1	impacted					862:869	impacted	862:869	speed significantly impacted the process responses and extrudate properties	842:916	The lentil variety, barrel temperature, and screw speed significantly impacted the process responses and extrudate properties.					
33565641	7	31	dep	3.0	1407:1409	arg1	to					1404:1405	to	1404:1405	to	1404:1405	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	5	32	theme	highest	1033:1039	arg1	speed					1047:1051	the highest screw speed	1029:1051	the highest screw speed (250 rpm)	1029:1061	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	5	32	theme	highest	1033:1039	arg1	250 rpm					1054:1060	250 rpm	1054:1060	250 rpm	1054:1060	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	0	33	from	varieties	40:48	arg1	flours					18:23	Whole seed lentil flours	0:23	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea)	0:79	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	3	34	from	differences	547:557	arg1	mPa.s					739:743	123.3 to 179.7 mPa.s	724:743	123.3 to 179.7 mPa.s	724:743	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	temperature					677:687	gelatinization temperature	662:687	gelatinization temperature (71.7 to 74.6 °C)	662:705	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	starch					579:584	their starch	573:584	their starch (48.7% to 50.9%)	573:601	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	viscosity					713:721	peak viscosity	708:721	peak viscosity (123.3 to 179.7 mPa.s)	708:744	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	content					638:644	fat content	634:644	fat content	634:644	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	%					658:658	1.3% to 1.9%	647:658	1.3% to 1.9%	647:658	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	%					600:600	48.7% to 50.9%	587:600	48.7% to 50.9%	587:600	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	%					626:626	20.4% to 22.7%	613:626	20.4% to 22.7%	613:626	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	74.6 °C					698:704	74.6 °C	698:704	74.6 °C	698:704	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	temperature					759:769	melting temperature	751:769	melting temperature	751:769	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	34	from	differences	547:557	arg1	protein					604:610	protein	604:610	protein (20.4% to 22.7%)	604:627	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	4	35	dep	process	875:881	arg1	responses					883:891	responses	883:891	responses	883:891	The lentil variety, barrel temperature, and screw speed significantly impacted the process responses and extrudate properties.					
33565641	3	36	theme	peak	708:711	arg1	mPa.s					739:743	123.3 to 179.7 mPa.s	724:743	123.3 to 179.7 mPa.s	724:743	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	36	theme	peak	708:711	arg1	viscosity					713:721	peak viscosity	708:721	peak viscosity (123.3 to 179.7 mPa.s)	708:744	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	9	37	from	impacts	1678:1684	arg1	properties					1693:1702	the properties	1689:1702	the properties of their extrudates	1689:1722	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	9	38	theme	extrudates	1713:1722	arg1	properties					1693:1702	the properties	1689:1702	the properties of their extrudates	1689:1722	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	0	39	theme	Whole	0:4	arg1	flours					18:23	Whole seed lentil flours	0:23	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea)	0:79	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	8	40	theme	direct-expanded	1506:1520	arg1	extrudates					1522:1531	direct-expanded extrudates	1506:1531	direct-expanded extrudates	1506:1531	Richlea variety was the most suitable for making direct-expanded extrudates among the varieties studied.					
33565641	2	41	theme	speed	364:368	arg1	effects					301:307	The effects	297:307	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics	297:443	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	0	42	theme	lentil	11:16	arg1	flours					18:23	Whole seed lentil flours	0:23	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea)	0:79	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	43	dep	varieties	40:48	arg1	Crimson					59:65	Crimson	59:65	Crimson	59:65	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	43	dep	varieties	40:48	arg1	Richlea					72:78	Richlea	72:78	Richlea	72:78	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	43	dep	varieties	40:48	arg1	Brewer					51:56	Brewer	51:56	Brewer	51:56	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	43	dep	varieties	40:48	arg1	varieties					40:48	different varieties	30:48	different varieties (Brewer, Crimson, and Richlea)	30:79	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	3	44	theme	melting	751:757	arg1	temperature					759:769	melting temperature	751:769	melting temperature	751:769	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	9	45	from	varieties	1633:1641	arg1	flours					1611:1616	flours	1611:1616	flours from the three varieties of lentils	1611:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	9	45	from	varieties	1633:1641	arg1	properties					1597:1606	the properties	1593:1606	the properties of flours from the three varieties of lentils	1593:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	4	46	theme	lentil	796:801	arg1	variety					803:809	The lentil variety	792:809	The lentil variety	792:809	The lentil variety, barrel temperature, and screw speed significantly impacted the process responses and extrudate properties.					
33565641	2	47	dep	speed	364:368	arg1	200					376:378	200	376:378	200	376:378	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	2	47	dep	speed	364:368	arg1	250 rpm					385:391	250 rpm	385:391	250 rpm	385:391	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	2	48	theme	barrel	312:317	arg1	temperature					319:329	barrel temperature	312:329	barrel temperature (110, 125, and 140 °C)	312:352	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	6	49	theme	solubility	1140:1149	arg1	index					1151:1155	the highest water solubility index	1122:1155	the highest water solubility index (45.4%)	1122:1163	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	49	theme	solubility	1140:1149	arg1	%					1162:1162	45.4%	1158:1162	45.4%	1158:1162	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	5	50	theme	highest	953:959	arg1	ratio					971:975	the highest expansion ratio	949:975	the highest expansion ratio	949:975	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	12	51	theme	chemical	2124:2131	arg1	composition					2133:2143	the chemical composition	2120:2143	the chemical composition	2120:2143	The data prove the importance of understanding the chemical composition, pasting, and thermal properties to select the appropriate varieties for extrusion processing.					
33565641	6	52	theme	peak	1206:1209	arg1	viscosity					1211:1219	peak viscosity	1206:1219	peak viscosity	1206:1219	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	5	53	theme	lowest	997:1002	arg1	temperature					1004:1014	the lowest temperature	993:1014	the lowest temperature (110 °C)	993:1023	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	5	53	theme	lowest	997:1002	arg1	110 °C					1017:1022	110 °C	1017:1022	110 °C	1017:1022	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	11	54	from	study	1917:1921	arg1	useful					1926:1931	useful	1926:1931	useful	1926:1931	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	11	54	from	study	1917:1921	arg1	information					1895:1905	The information	1891:1905	The information from this study	1891:1921	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	11	55	theme	PRACTICAL	1868:1876	arg1	APPLICATION					1878:1888	PRACTICAL APPLICATION	1868:1888	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.	1868:2071	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	10	56	theme	different	1776:1784	arg1	varieties					1786:1794	different varieties	1776:1794	different varieties	1776:1794	Therefore, determining the properties of flours of different varieties is useful to select the appropriate varieties for extrusion processing.					
33565641	6	57	theme	highest	1179:1185	arg1	content					1194:1200	starch content	1187:1200	starch content	1187:1200	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	1	58	theme	varieties	242:250	arg1	properties					174:183	The properties	170:183	The properties	170:183	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	2	59	from	effects	301:307	arg1	responses					405:413	process responses	397:413	process responses	397:413	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	2	59	from	effects	301:307	arg1	characteristics					429:443	extrudate characteristics	419:443	extrudate characteristics	419:443	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	10	60	theme	flours	1766:1771	arg1	properties					1752:1761	the properties	1748:1761	the properties of flours of different varieties	1748:1794	Therefore, determining the properties of flours of different varieties is useful to select the appropriate varieties for extrusion processing.					
33565641	12	61	theme	appropriate	2192:2202	arg1	varieties					2204:2212	the appropriate varieties	2188:2212	the appropriate varieties for extrusion processing	2188:2237	The data prove the importance of understanding the chemical composition, pasting, and thermal properties to select the appropriate varieties for extrusion processing.					
33565641	10	62	theme	varieties	1786:1794	arg1	flours					1766:1771	flours	1766:1771	flours of different varieties	1766:1794	Therefore, determining the properties of flours of different varieties is useful to select the appropriate varieties for extrusion processing.					
33565641	3	63	theme	%	617:617	arg1	%					626:626	20.4% to 22.7%	613:626	20.4% to 22.7%	613:626	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	63	theme	%	617:617	arg1	protein					604:610	protein	604:610	protein (20.4% to 22.7%)	604:627	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	6	64	dep	content	1245:1251	arg1	the					1226:1228	the	1226:1228	the	1226:1228	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	11	65	theme	food	1941:1944	arg1	industry					1946:1953	the food industry	1937:1953	the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products	1937:2070	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	6	66	theme	protein	1237:1243	arg1	content					1245:1251	protein content	1237:1251	protein content	1237:1251	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	5	67	theme	lentil	925:930	arg1	flours					932:937	Whole lentil flours	919:937	Whole lentil flours	919:937	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	11	68	theme	lentil	1981:1986	arg1	varieties					1988:1996	the appropriate lentil varieties	1965:1996	the appropriate lentil varieties	1965:1996	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	6	69	theme	expansion	1096:1104	arg1	3.6					1113:1115	3.6	1113:1115	3.6	1113:1115	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	69	theme	expansion	1096:1104	arg1	ratio					1106:1110	the highest expansion ratio	1084:1110	the highest expansion ratio (3.6)	1084:1116	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	1	70	theme	flours	188:193	arg1	properties					174:183	The properties	170:183	The properties	170:183	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	2	71	theme	corotating	468:477	arg1	extruder					490:497	a corotating twin-screw extruder	466:497	a corotating twin-screw extruder	466:497	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	3	72	theme	lentils	523:529	arg1	lentils					523:529	lentils	523:529	lentils	523:529	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	72	theme	lentils	523:529	arg1	varieties					510:518	The three varieties	500:518	The three varieties of lentils	500:529	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	73	dep	119.7 °C	781:788	arg1	to					778:779	to	778:779	to	778:779	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	1	74	theme	extrusion	199:207	arg1	characteristics					209:223	extrusion characteristics	199:223	extrusion characteristics	199:223	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	7	75	theme	lowest	1318:1323	arg1	ratio					1335:1339	the lowest expansion ratio	1314:1339	the lowest expansion ratio	1314:1339	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	7	76	dep	3.6	1382:1384	arg1	to					1379:1380	to	1379:1380	to	1379:1380	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	5	77	theme	screw	1041:1045	arg1	speed					1047:1051	the highest screw speed	1029:1051	the highest screw speed (250 rpm)	1029:1061	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	5	77	theme	screw	1041:1045	arg1	250 rpm					1054:1060	250 rpm	1054:1060	250 rpm	1054:1060	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	3	78	dep	%	658:658	arg1	to					652:653	to	652:653	to	652:653	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	5	79	dep	3.6	985:987	arg1	to					982:983	to	982:983	to	982:983	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	9	80	from	properties	1597:1606	arg1	lentils					1646:1652	lentils	1646:1652	lentils	1646:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	9	80	from	properties	1597:1606	arg1	varieties					1633:1641	the three varieties	1623:1641	the three varieties of lentils	1623:1652	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	10	81	theme	appropriate	1820:1830	arg1	varieties					1832:1840	the appropriate varieties	1816:1840	the appropriate varieties for extrusion processing	1816:1865	Therefore, determining the properties of flours of different varieties is useful to select the appropriate varieties for extrusion processing.					
33565641	6	82	theme	water	1134:1138	arg1	index					1151:1155	the highest water solubility index	1122:1155	the highest water solubility index (45.4%)	1122:1163	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	82	theme	water	1134:1138	arg1	%					1162:1162	45.4%	1158:1162	45.4%	1158:1162	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	3	83	theme	significant	535:545	arg1	differences					547:557	significant differences	535:557	significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature	535:769	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	83	theme	significant	535:545	arg1	p < 0.05					560:567	p < 0.05	560:567	p < 0.05	560:567	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	84	dep	179.7	733:737	arg1	to					730:731	to	730:731	to	730:731	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	7	85	theme	Brewer	1289:1294	arg1	Meanwhile					1278:1286	Meanwhile	1278:1286	Meanwhile	1278:1286	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	7	85	theme	Brewer	1289:1294	arg1	variety					1296:1302	Brewer variety	1289:1302	Brewer variety	1289:1302	Meanwhile, Brewer variety exhibited the lowest expansion ratio (1.9 to 3.0) compared to Richlea (2.5 to 3.6) and Crimson (2.4 to 3.0) in most of the extrusion conditions studied.					
33565641	9	86	theme	significant	1666:1676	arg1	impacts					1678:1684	significant impacts	1666:1684	significant impacts on the properties of their extrudates	1666:1722	The significant differences in the properties of flours from the three varieties of lentils resulted in significant impacts on the properties of their extrudates.					
33565641	5	87	theme	Whole	919:923	arg1	flours					932:937	Whole lentil flours	919:937	Whole lentil flours	919:937	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	6	88	theme	highest	1088:1094	arg1	3.6					1113:1115	3.6	1113:1115	3.6	1113:1115	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	88	theme	highest	1088:1094	arg1	ratio					1106:1110	the highest expansion ratio	1084:1110	the highest expansion ratio (3.6)	1084:1116	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	1	89	dep	studied	288:294	arg1	Crimson					261:267	Crimson	261:267	Crimson	261:267	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	1	89	dep	studied	288:294	arg1	Richlea					274:280	Richlea	274:280	Richlea	274:280	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	1	89	dep	studied	288:294	arg1	Brewer					253:258	Brewer	253:258	Brewer	253:258	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
33565641	11	90	theme	products	2063:2070	arg1	development					2032:2042	the development	2028:2042	the development of direct-expanded products	2028:2070	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	2	91	dep	temperature	319:329	arg1	125					337:339	125	337:339	125	337:339	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	2	91	dep	temperature	319:329	arg1	140 °C					346:351	140 °C	346:351	140 °C	346:351	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	2	92	theme	extrudate	419:427	arg1	characteristics					429:443	extrudate characteristics	419:443	extrudate characteristics	419:443	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	5	93	dep	exhibited	939:947	arg1	3.6					985:987	3.6	985:987	3.6	985:987	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	11	94	dep	APPLICATION	1878:1888	arg1	useful					1926:1931	useful	1926:1931	useful	1926:1931	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	11	94	dep	APPLICATION	1878:1888	arg1	information					1895:1905	The information	1891:1905	The information from this study	1891:1921	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	0	95	theme	seed	6:9	arg1	flours					18:23	Whole seed lentil flours	0:23	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea)	0:79	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	3	96	dep	74.6 °C	698:704	arg1	to					695:696	to	695:696	to	695:696	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	97	dep	%	600:600	arg1	to					593:594	to	593:594	to	593:594	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	4	98	theme	barrel	812:817	arg1	temperature					819:829	barrel temperature	812:829	barrel temperature	812:829	The lentil variety, barrel temperature, and screw speed significantly impacted the process responses and extrudate properties.					
33565641	11	99	theme	appropriate	1969:1979	arg1	varieties					1988:1996	the appropriate lentil varieties	1965:1996	the appropriate lentil varieties	1965:1996	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	2	100	theme	screw	358:362	arg1	speed					364:368	screw speed	358:368	screw speed (150, 200, and 250 rpm)	358:392	The effects of barrel temperature (110, 125, and 140 °C) and screw speed (150, 200, and 250 rpm) on process responses and extrudate characteristics were evaluated using a corotating twin-screw extruder.					
33565641	0	101	theme	different	30:38	arg1	Crimson					59:65	Crimson	59:65	Crimson	59:65	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	101	theme	different	30:38	arg1	Richlea					72:78	Richlea	72:78	Richlea	72:78	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	101	theme	different	30:38	arg1	Brewer					51:56	Brewer	51:56	Brewer	51:56	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	0	101	theme	different	30:38	arg1	varieties					40:48	different varieties	30:48	different varieties (Brewer, Crimson, and Richlea)	30:79	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	10	102	theme	extrusion	1846:1854	arg1	processing					1856:1865	extrusion processing	1846:1865	extrusion processing	1846:1865	Therefore, determining the properties of flours of different varieties is useful to select the appropriate varieties for extrusion processing.					
33565641	6	103	contain	had	1171:1173	arg2	viscosity					1211:1219	peak viscosity	1206:1219	peak viscosity	1206:1219	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	103	contain	had	1171:1173	arg2	content					1194:1200	starch content	1187:1200	starch content	1187:1200	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	6	103	contain	had	1171:1173	arg1	it					1168:1169	it	1168:1169	it	1168:1169	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	3	104	theme	%	591:591	arg1	starch					579:584	their starch	573:584	their starch (48.7% to 50.9%)	573:601	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	3	104	theme	%	591:591	arg1	%					600:600	48.7% to 50.9%	587:600	48.7% to 50.9%	587:600	The three varieties of lentils had significant differences (p < 0.05) in their starch (48.7% to 50.9%), protein (20.4% to 22.7%), and fat content (1.3% to 1.9%), gelatinization temperature (71.7 to 74.6 °C), peak viscosity (123.3 to 179.7 mPa.s), and melting temperature (113.6 to 119.7 °C).					
33565641	5	105	theme	expansion	961:969	arg1	ratio					971:975	the highest expansion ratio	949:975	the highest expansion ratio	949:975	Whole lentil flours exhibited the highest expansion ratio (3.0 to 3.6) at the lowest temperature (110 °C) and the highest screw speed (250 rpm).					
33565641	6	106	dep	content	1194:1200	arg1	the					1175:1177	the	1175:1177	the	1175:1177	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	11	107	theme	processing	2002:2011	arg1	conditions					2013:2022	processing conditions	2002:2022	processing conditions	2002:2022	PRACTICAL APPLICATION: The information from this study is useful for the food industry to select the appropriate lentil varieties and processing conditions for the development of direct-expanded products.					
33565641	0	108	from	variations	106:115	arg1	characteristics					136:150	their expansion characteristics	120:150	their expansion characteristics	120:150	Whole seed lentil flours from different varieties (Brewer, Crimson, and Richlea) demonstrated significant variations in their expansion characteristics during extrusion.					
33565641	6	109	theme	starch	1187:1192	arg1	content					1194:1200	starch content	1187:1200	starch content	1187:1200	Richlea variety had the highest expansion ratio (3.6) and the highest water solubility index (45.4%) as it had the highest starch content and peak viscosity, and the lowest protein content and melting temperature.					
33565641	1	110	theme	lentil	235:240	arg1	varieties					242:250	three lentil varieties	229:250	three lentil varieties	229:250	The properties of flours and extrusion characteristics, of three lentil varieties (Brewer, Crimson, and Richlea) were studied.					
32337231	11	0	theme	non-Fickian	1682:1692	arg1	mechanism					1712:1720	non-Fickian diffusion release mechanism	1682:1720	non-Fickian diffusion release mechanism	1682:1720	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	12	1	used	used	1797:1800	arg2	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	1	used	used	1797:1800	arg2	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	4	2	theme	cross-linked	365:376	arg1	release					401:407	a drug release	394:407	a drug release sustaining excipient in microsphere formulations of theophylline	394:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	4	2	theme	cross-linked	365:376	arg1	starch					384:389	epichlorohydrin cross-linked enset starch	349:389	epichlorohydrin cross-linked enset starch	349:389	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	11	3	theme	kinetic	1663:1669	arg1	model					1671:1675	the Higuchi square root kinetic model	1639:1675	the Higuchi square root kinetic model	1639:1675	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	5	4	theme	enset	485:489	arg1	starch					491:496	Extracted enset starch	475:496	Extracted enset starch	475:496	Extracted enset starch was cross-linked using epichlorohydrin as a cross-linking agent.					
32337231	12	5	theme	pharmaceutical	1844:1857	arg1	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	5	theme	pharmaceutical	1844:1857	arg1	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	11	6	theme	optimized	1608:1616	arg1	formulation					1618:1628	the optimized formulation	1604:1628	the optimized formulation	1604:1628	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	6	7	theme	duration	619:626	arg1	effect					567:572	The effect	563:572	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method	563:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	6	8	theme	factorial	813:821	arg1	design					823:828	the two-level full factorial design	794:828	the two-level full factorial design	794:828	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	3	9	theme	Musaceae	259:266	arg1	family					249:254	the family	245:254	the family of Musaceae	245:266	cheeseman which belongs to the family of Musaceae is one of the main sources of starch in Ethiopia.					
32337231	12	10	theme	drug-release-sustaining	1820:1842	arg1	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	10	theme	drug-release-sustaining	1820:1842	arg1	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	6	11	theme	cross-linker	577:588	arg1	concentration					590:602	cross-linker concentration	577:602	cross-linker concentration	577:602	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	12	12	link	cross-linked	1764:1775	arg1	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	12	link	cross-linked	1764:1775	arg1	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	9	13	theme	3.82 h.	1280:1286	arg1	concentration					1230:1242	epichlorohydrin concentration	1214:1242	epichlorohydrin concentration of 13.70%	1214:1252	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	13	theme	3.82 h.	1280:1286	arg1	time					1272:1275	cross-linking time	1258:1275	cross-linking time of 3.82 h.	1258:1286	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	5	14	theme	cross-linking	542:554	arg1	agent					556:560	a cross-linking agent	540:560	a cross-linking agent	540:560	Extracted enset starch was cross-linked using epichlorohydrin as a cross-linking agent.					
32337231	5	14	theme	cross-linking	542:554	arg1	epichlorohydrin					521:535	epichlorohydrin	521:535	epichlorohydrin	521:535	Extracted enset starch was cross-linked using epichlorohydrin as a cross-linking agent.					
32337231	4	15	theme	microsphere	433:443	arg1	formulations					445:456	microsphere formulations	433:456	microsphere formulations of theophylline	433:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	11	16	theme	diffusion	1694:1702	arg1	mechanism					1712:1720	non-Fickian diffusion release mechanism	1682:1720	non-Fickian diffusion release mechanism	1682:1720	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	3	17	from	sources	287:293	arg1	Ethiopia					308:315	Ethiopia	308:315	Ethiopia	308:315	cheeseman which belongs to the family of Musaceae is one of the main sources of starch in Ethiopia.					
32337231	12	18	theme	enset	1777:1781	arg1	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	18	theme	enset	1777:1781	arg1	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	19	theme	alternative	1808:1818	arg1	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	19	theme	alternative	1808:1818	arg1	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	11	20	theme	minimum	1540:1546	arg1	release					1554:1560	minimum burst release	1540:1560	minimum burst release	1540:1560	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	6	21	theme	rate	702:705	arg1	degree					666:671	the degree	662:671	the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method	662:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	3	22	theme	starch	298:303	arg1	sources					287:293	the main sources	278:293	the main sources of starch in Ethiopia	278:315	cheeseman which belongs to the family of Musaceae is one of the main sources of starch in Ethiopia.					
32337231	0	23	theme	Cross-Linked	61:72	arg1	Enset					74:78	Epichlorohydrin Cross-Linked Enset	45:78	Epichlorohydrin Cross-Linked Enset	45:78	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	1	24	theme	Drug	129:132	arg1	Release					134:140	Drug Release	129:140	Drug Release Sustaining Excipient in Microsphere Formulation	129:188	Cheeseman) Starch as Drug Release Sustaining Excipient in Microsphere Formulation.					
32337231	12	25	theme	study	1743:1747	arg1	finding					1727:1733	The finding	1723:1733	The finding of this study	1723:1747	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	3	26	theme	sources	287:293	arg1	sources					287:293	the main sources	278:293	the main sources of starch in Ethiopia	278:315	cheeseman which belongs to the family of Musaceae is one of the main sources of starch in Ethiopia.					
32337231	3	26	theme	sources	287:293	arg1	one					271:273	one	271:273	one	271:273	cheeseman which belongs to the family of Musaceae is one of the main sources of starch in Ethiopia.					
32337231	6	27	theme	temperature	647:657	arg1	effect					567:572	The effect	563:572	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method	563:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	9	28	theme	cross-linking	1353:1365	arg1	rate					1394:1397	drug release rate	1381:1397	drug release rate of 28.00 h1/2	1381:1411	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	28	theme	cross-linking	1353:1365	arg1	degree					1343:1348	the degree	1339:1348	the degree of cross-linking of 74.70%	1339:1375	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	7	29	theme	drug	992:995	arg1	rate					1005:1008	drug release rate	992:1008	drug release rate	992:1008	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	6	30	theme	microspheres	710:721	arg1	rate					702:705	release rate	694:705	release rate	694:705	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	6	30	theme	microspheres	710:721	arg1	cross-linking					676:688	cross-linking	676:688	cross-linking	676:688	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	8	31	theme	composite	1103:1111	arg1	design					1113:1118	the central composite design	1091:1118	the central composite design	1091:1118	Thus, the effects of these two factors were further studied and optimized using the central composite design.					
32337231	7	32	theme	cross-linking	974:986	arg1	degree					964:969	the degree	960:969	the degree of cross-linking	960:986	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	7	32	theme	cross-linking	974:986	arg1	rate					1005:1008	drug release rate	992:1008	drug release rate	992:1008	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	11	33	theme	optimum	1505:1511	arg1	formulation					1513:1523	the optimum formulation	1501:1523	the optimum formulation	1501:1523	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	9	34	theme	central	1152:1158	arg1	design					1170:1175	central composite design	1152:1175	central composite design	1152:1175	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	35	theme	release	1386:1392	arg1	rate					1394:1397	drug release rate	1381:1397	drug release rate of 28.00 h1/2	1381:1411	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	11	36	theme	square	1651:1656	arg1	model					1671:1675	the Higuchi square root kinetic model	1639:1675	the Higuchi square root kinetic model	1639:1675	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	11	37	theme	Higuchi	1643:1649	arg1	model					1671:1675	the Higuchi square root kinetic model	1639:1675	the Higuchi square root kinetic model	1639:1675	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	12	38	theme	microsphere	1872:1882	arg1	formulation					1884:1894	microsphere formulation	1872:1894	microsphere formulation	1872:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	4	39	link	cross-linked	365:376	arg1	release					401:407	a drug release	394:407	a drug release sustaining excipient in microsphere formulations of theophylline	394:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	4	39	link	cross-linked	365:376	arg1	starch					384:389	epichlorohydrin cross-linked enset starch	349:389	epichlorohydrin cross-linked enset starch	349:389	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	0	40	dep	Preparation	0:10	arg1	Ensete					81:86	Ensete	81:86	Ensete	81:86	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	0	40	dep	Preparation	0:10	arg1	Welw					101:104	Welw	101:104	Welw	101:104	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	6	41	theme	solvent	744:750	arg1	method					764:769	emulsion solvent evaporation method	735:769	emulsion solvent evaporation method	735:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	4	42	theme	enset	378:382	arg1	release					401:407	a drug release	394:407	a drug release sustaining excipient in microsphere formulations of theophylline	394:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	4	42	theme	enset	378:382	arg1	starch					384:389	epichlorohydrin cross-linked enset starch	349:389	epichlorohydrin cross-linked enset starch	349:389	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	10	43	theme	points	1444:1449	arg1	validity					1418:1425	The validity	1414:1425	The validity of these optimal points	1414:1449	The validity of these optimal points was confirmed experimentally.					
32337231	9	44	theme	epichlorohydrin	1214:1228	arg1	concentration					1230:1242	epichlorohydrin concentration	1214:1242	epichlorohydrin concentration of 13.70%	1214:1252	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	7	45	theme	significant	925:935	arg1	factors					937:943	the most significant factors	916:943	the most significant factors affecting both the degree of cross-linking and drug release rate	916:1008	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	7	45	theme	significant	925:935	arg1	concentration					848:860	the concentration	844:860	the concentration of epichlorohydrin and duration of cross-linking	844:909	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	9	46	theme	design	1170:1175	arg1	method					1142:1147	the numerical method	1128:1147	the numerical method of central composite design	1128:1175	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	11	47	theme	sustained	1567:1575	arg1	release					1577:1583	sustained release	1567:1583	sustained release for 12 h.	1567:1593	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	8	48	theme	factors	1042:1048	arg1	effects					1021:1027	the effects	1017:1027	the effects of these two factors	1017:1048	Thus, the effects of these two factors were further studied and optimized using the central composite design.					
32337231	9	49	theme	%	1252:1252	arg1	concentration					1230:1242	epichlorohydrin concentration	1214:1242	epichlorohydrin concentration of 13.70%	1214:1252	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	49	theme	%	1252:1252	arg1	time					1272:1275	cross-linking time	1258:1275	cross-linking time of 3.82 h.	1258:1286	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	7	50	theme	cross-linking	897:909	arg1	duration					885:892	duration	885:892	duration	885:892	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	7	50	theme	cross-linking	897:909	arg1	epichlorohydrin					865:879	epichlorohydrin	865:879	epichlorohydrin	865:879	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	1	51	theme	Microsphere	166:176	arg1	Formulation					178:188	Microsphere Formulation	166:188	Microsphere Formulation	166:188	Cheeseman) Starch as Drug Release Sustaining Excipient in Microsphere Formulation.					
32337231	4	52	theme	epichlorohydrin	349:363	arg1	release					401:407	a drug release	394:407	a drug release sustaining excipient in microsphere formulations of theophylline	394:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	4	52	theme	epichlorohydrin	349:363	arg1	starch					384:389	epichlorohydrin cross-linked enset starch	349:389	epichlorohydrin cross-linked enset starch	349:389	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	6	53	theme	cross-linking	605:617	arg1	duration					619:626	cross-linking duration	605:626	cross-linking duration	605:626	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	10	54	theme	optimal	1436:1442	arg1	points					1444:1449	these optimal points	1430:1449	these optimal points	1430:1449	The validity of these optimal points was confirmed experimentally.					
32337231	9	55	theme	cross-linking	1258:1270	arg1	time					1272:1275	cross-linking time	1258:1275	cross-linking time of 3.82 h.	1258:1286	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	7	56	theme	duration	885:892	arg1	factors					937:943	the most significant factors	916:943	the most significant factors affecting both the degree of cross-linking and drug release rate	916:1008	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	7	56	theme	duration	885:892	arg1	concentration					848:860	the concentration	844:860	the concentration of epichlorohydrin and duration of cross-linking	844:909	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	6	57	theme	concentration	590:602	arg1	effect					567:572	The effect	563:572	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method	563:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	11	58	theme	burst	1548:1552	arg1	release					1554:1560	minimum burst release	1540:1560	minimum burst release	1540:1560	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	7	59	theme	epichlorohydrin	865:879	arg1	factors					937:943	the most significant factors	916:943	the most significant factors affecting both the degree of cross-linking and drug release rate	916:1008	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	7	59	theme	epichlorohydrin	865:879	arg1	concentration					848:860	the concentration	844:860	the concentration of epichlorohydrin and duration of cross-linking	844:909	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	6	60	theme	two-level	798:806	arg1	design					823:828	the two-level full factorial design	794:828	the two-level full factorial design	794:828	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	3	61	theme	main	282:285	arg1	sources					287:293	the main sources	278:293	the main sources of starch in Ethiopia	278:315	cheeseman which belongs to the family of Musaceae is one of the main sources of starch in Ethiopia.					
32337231	4	62	theme	drug	396:399	arg1	release					401:407	a drug release	394:407	a drug release sustaining excipient in microsphere formulations of theophylline	394:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	4	62	theme	drug	396:399	arg1	starch					384:389	epichlorohydrin cross-linked enset starch	349:389	epichlorohydrin cross-linked enset starch	349:389	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	6	63	theme	cross-linking	676:688	arg1	degree					666:671	the degree	662:671	the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method	662:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	6	64	theme	full	808:811	arg1	design					823:828	the two-level full factorial design	794:828	the two-level full factorial design	794:828	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	9	65	theme	optimal	1300:1306	arg1	conditions					1308:1317	these optimal conditions	1294:1317	these optimal conditions	1294:1317	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	12	66	theme	cross-linked	1764:1775	arg1	starch					1783:1788	cross-linked enset starch	1764:1788	cross-linked enset starch	1764:1788	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	12	66	theme	cross-linked	1764:1775	arg1	excipient					1859:1867	an alternative drug-release-sustaining pharmaceutical excipient	1805:1867	an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation	1805:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	11	67	theme	formulation	1513:1523	arg1	microspheres					1485:1496	The microspheres	1481:1496	The microspheres of the optimum formulation	1481:1523	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	0	68	theme	Enset	74:78	arg1	Optimization					13:24	Optimization	13:24	Optimization	13:24	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	0	68	theme	Enset	74:78	arg1	Evaluation					31:40	Evaluation	31:40	Evaluation	31:40	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	0	68	theme	Enset	74:78	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	6	69	theme	cross-linking	633:645	arg1	temperature					647:657	cross-linking temperature	633:657	cross-linking temperature	633:657	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	4	70	theme	theophylline	461:472	arg1	formulations					445:456	microsphere formulations	433:456	microsphere formulations of theophylline	433:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	6	71	theme	emulsion	735:742	arg1	method					764:769	emulsion solvent evaporation method	735:769	emulsion solvent evaporation method	735:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	8	72	theme	central	1095:1101	arg1	design					1113:1118	the central composite design	1091:1118	the central composite design	1091:1118	Thus, the effects of these two factors were further studied and optimized using the central composite design.					
32337231	12	73	from	excipient	1859:1867	arg1	formulation					1884:1894	microsphere formulation	1872:1894	microsphere formulation	1872:1894	The finding of this study suggested that cross-linked enset starch can be used as an alternative drug-release-sustaining pharmaceutical excipient in microsphere formulation.					
32337231	7	74	theme	release	997:1003	arg1	rate					1005:1008	drug release rate	992:1008	drug release rate	992:1008	Accordingly, the concentration of epichlorohydrin and duration of cross-linking were the most significant factors affecting both the degree of cross-linking and drug release rate.					
32337231	0	75	dep	Ensete	81:86	arg1	ventricosum					88:98	Ensete ventricosum	81:98	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)	0:106	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	9	76	theme	numerical	1132:1140	arg1	method					1142:1147	the numerical method	1128:1147	the numerical method of central composite design	1128:1175	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	77	theme	%	1375:1375	arg1	cross-linking					1353:1365	cross-linking	1353:1365	cross-linking of 74.70%	1353:1375	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	5	78	theme	Extracted	475:483	arg1	starch					491:496	Extracted enset starch	475:496	Extracted enset starch	475:496	Extracted enset starch was cross-linked using epichlorohydrin as a cross-linking agent.					
32337231	6	79	theme	release	694:700	arg1	rate					702:705	release rate	694:705	release rate	694:705	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	9	80	theme	drug	1381:1384	arg1	rate					1394:1397	drug release rate	1381:1397	drug release rate of 28.00 h1/2	1381:1411	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	81	theme	composite	1160:1168	arg1	design					1170:1175	central composite design	1152:1175	central composite design	1152:1175	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	0	82	theme	Epichlorohydrin	45:59	arg1	Enset					74:78	Epichlorohydrin Cross-Linked Enset	45:78	Epichlorohydrin Cross-Linked Enset	45:78	Preparation, Optimization, and Evaluation of Epichlorohydrin Cross-Linked Enset (Ensete ventricosum (Welw.)					
32337231	4	83	from	excipient	420:428	arg1	formulations					445:456	microsphere formulations	433:456	microsphere formulations of theophylline	433:472	This study aimed at evaluating epichlorohydrin cross-linked enset starch as a drug release sustaining excipient in microsphere formulations of theophylline.					
32337231	9	84	theme	28.00 h1/2	1402:1411	arg1	rate					1394:1397	drug release rate	1381:1397	drug release rate of 28.00 h1/2	1381:1411	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	9	84	theme	28.00 h1/2	1402:1411	arg1	degree					1343:1348	the degree	1339:1348	the degree of cross-linking of 74.70%	1339:1375	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	6	85	from	effect	567:572	arg1	degree					666:671	the degree	662:671	the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method	662:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	6	86	theme	evaporation	752:762	arg1	method					764:769	emulsion solvent evaporation method	735:769	emulsion solvent evaporation method	735:769	The effect of cross-linker concentration, cross-linking duration, and cross-linking temperature on the degree of cross-linking and release rate of microspheres prepared by emulsion solvent evaporation method was investigated using the two-level full factorial design.					
32337231	2	87	theme	Ensete	191:196	arg1	Welw					211:214	Welw	211:214	Welw	211:214	Ensete ventricosum (Welw.)					
32337231	2	87	theme	Ensete	191:196	arg1	ventricosum					198:208	Ensete ventricosum	191:208	Ensete ventricosum (Welw.)	191:216	Ensete ventricosum (Welw.)					
32337231	9	88	theme	optimal	1182:1188	arg1	points					1190:1195	the optimal points	1178:1195	the optimal points	1178:1195	As per the numerical method of central composite design, the optimal points were obtained at epichlorohydrin concentration of 13.70% and cross-linking time of 3.82 h. Under these optimal conditions, the model predicts the degree of cross-linking of 74.70% and drug release rate of 28.00 h1/2.					
32337231	11	89	theme	release	1704:1710	arg1	mechanism					1712:1720	non-Fickian diffusion release mechanism	1682:1720	non-Fickian diffusion release mechanism	1682:1720	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
32337231	11	90	theme	root	1658:1661	arg1	model					1671:1675	the Higuchi square root kinetic model	1639:1675	the Higuchi square root kinetic model	1639:1675	The microspheres of the optimum formulation also exhibited minimum burst release with sustained release for 12 h. Besides, the optimized formulation followed the Higuchi square root kinetic model with non-Fickian diffusion release mechanism.					
34308934	6	0	theme	afferent	952:959	arg1	activity					961:968	Vagal afferent activity	946:968	Vagal afferent activity	946:968	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	1	1	theme	beneficial	266:275	arg1	effects					277:283	several well-established beneficial effects	241:283	several well-established beneficial effects in the host	241:295	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	3	2	theme	afferent	547:554	arg1	pathway					556:562	the vagal afferent pathway	537:562	the vagal afferent pathway	537:562	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	11	3	theme	lipid	1685:1689	arg1	metabolism					1691:1700	lipid metabolism	1685:1700	lipid metabolism	1685:1700	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	11	3	theme	lipid	1685:1689	arg1	profiles					1652:1659	the metabolic profiles	1638:1659	the metabolic profiles	1638:1659	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	1	4	theme	predominant	184:194	arg1	oligosaccharides					196:211	the predominant oligosaccharides	180:211	the predominant oligosaccharides found in human milk	180:231	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	12	5	theme	metabolic	1864:1872	arg1	challenge					1874:1882	a metabolic challenge	1862:1882	a metabolic challenge	1862:1882	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	2	6	from	phenotype	364:372	arg1	mice					395:398	high-fat (HF)-fed mice	377:398	high-fat (HF)-fed mice	377:398	It has previously been shown that 2'-FL can improve the metabolic phenotype in high-fat (HF)-fed mice.					
34308934	6	7	theme	c-Fos	1020:1024	arg1	protein					1026:1032	c-Fos protein	1020:1032	c-Fos protein	1020:1032	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	12	8	theme	function	1821:1828	arg1	control					1798:1804	the control	1794:1804	the control of gut barrier function and metabolic homeostasis	1794:1854	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	4	9	theme	%	626:626	arg1	LF					620:621	LF	620:621	LF	620:621	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	4	9	theme	%	626:626	arg1	fat					628:630	10% fat	624:630	10% fat per kcal	624:639	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	10	10	theme	vagal	1509:1513	arg1	pathway					1524:1530	the vagal afferent pathway	1505:1530	the vagal afferent pathway	1505:1530	Signaling in the vagal afferent pathway was improved but there was no effect on cognitive function.					
34308934	1	11	contain	has	237:239	arg1	2'-FL					163:167	2'-FL	163:167	2'-FL	163:167	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	1	11	contain	has	237:239	arg1	2'-Fucosyllactose					144:160	2'-Fucosyllactose	144:160	2'-Fucosyllactose (2'-FL)	144:168	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	1	11	contain	has	237:239	arg2	effects					277:283	several well-established beneficial effects	241:283	several well-established beneficial effects in the host	241:295	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	1	12	theme	human	222:226	arg1	milk					228:231	human milk	222:231	human milk	222:231	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	12	13	theme	metabolic	1834:1842	arg1	homeostasis					1844:1854	metabolic homeostasis	1834:1854	metabolic homeostasis	1834:1854	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	4	14	theme	fat	653:655	arg1	%					651:651	45% fat	649:655	45% fat per kcal	649:664	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	1	15	located	found	213:217	arg1	milk					228:231	human milk	222:231	human milk	222:231	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	1	15	located	found	213:217	arg2	oligosaccharides					196:211	the predominant oligosaccharides	180:211	the predominant oligosaccharides found in human milk	180:231	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	5	16	theme	intestinal	795:804	arg1	permeability					806:817	intestinal permeability	795:817	intestinal permeability (ex vivo in Ussing chambers)	795:846	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	6	17	theme	peripheral	1066:1075	arg1	administration					1077:1090	peripheral administration	1066:1090	peripheral administration of cholecystokinin (CCK)	1066:1115	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	4	18	theme	HF	645:646	arg1	diet					667:670	either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet	602:670	diet	667:670	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	3	19	from	function	578:585	arg1	pathway					556:562	the vagal afferent pathway	537:562	the vagal afferent pathway	537:562	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	11	20	theme	vagal	1723:1727	arg1	pathway					1738:1744	the vagal afferent pathway	1719:1744	the vagal afferent pathway	1719:1744	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	5	21	dep	permeability	806:817	arg1	vivo					823:826	ex vivo	820:826	ex vivo in Ussing chambers	820:845	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	11	22	theme	gut	1662:1664	arg1	integrity					1674:1682	gut barrier integrity	1662:1682	gut barrier integrity	1662:1682	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	11	22	theme	gut	1662:1664	arg1	profiles					1652:1659	the metabolic profiles	1638:1659	the metabolic profiles	1638:1659	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	8	23	theme	intestinal	1232:1241	arg1	permeability					1243:1254	intestinal permeability	1232:1254	intestinal permeability	1232:1254	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	4	24	from	supplementation	688:702	arg1	diet					730:733	the diet	726:733	the diet for 8 weeks	726:745	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	5	25	theme	Ussing	831:836	arg1	chambers					838:845	Ussing chambers	831:845	Ussing chambers	831:845	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	12	26	from	utility	1774:1780	arg1	control					1798:1804	the control	1794:1804	the control of gut barrier function and metabolic homeostasis	1794:1854	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	4	27	theme	2'-FL	707:711	arg1	supplementation					688:702	supplementation	688:702	supplementation of 2'-FL (10% w/w) in the diet for 8 weeks	688:745	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	0	28	theme	afferent	118:125	arg1	pathway					127:133	the vagal afferent pathway	108:133	the vagal afferent pathway in mice	108:141	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	8	29	theme	increased	1274:1282	arg1	expression					1284:1293	significantly increased expression	1260:1293	significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine	1260:1375	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	0	30	theme	Human	0:4	arg1	supplementation					45:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	12	31	theme	2'-FL	1785:1789	arg1	utility					1774:1780	the utility	1770:1780	the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis	1770:1854	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	5	32	theme	gut	865:867	arg1	microbiome					869:878	gut microbiome	865:878	gut microbiome	865:878	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	0	33	theme	oligosaccharide	11:25	arg1	supplementation					45:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	9	34	theme	associated	1458:1467	arg1	metabolites					1479:1489	the associated microbial metabolites	1454:1489	the associated microbial metabolites	1454:1489	Additionally, 2'-FL led to changes in the gut microbiota composition and in the associated microbial metabolites.					
34308934	7	35	theme	energy	1189:1194	arg1	intake					1196:1201	energy intake	1189:1201	energy intake	1189:1201	2'-FL significantly attenuated the HF-induced increase in fat mass and energy intake.					
34308934	3	36	theme	improved	485:492	arg1	integrity					513:521	improved intestinal barrier integrity	485:521	improved intestinal barrier integrity	485:521	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	0	37	theme	gut	70:72	arg1	function					82:89	gut barrier function	70:89	gut barrier function	70:89	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	5	38	theme	microbial	884:892	arg1	metabolites					894:904	microbial metabolites	884:904	microbial metabolites	884:904	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	6	39	theme	immunohistochemical	987:1005	arg1	detection					1007:1015	immunohistochemical detection	987:1015	immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK)	987:1115	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	11	40	theme	2'-FL	1607:1611	arg1	supplementation					1613:1627	2'-FL supplementation	1607:1627	2'-FL supplementation	1607:1627	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	10	41	theme	cognitive	1572:1580	arg1	function					1582:1589	cognitive function	1572:1589	cognitive function	1572:1589	Signaling in the vagal afferent pathway was improved but there was no effect on cognitive function.					
34308934	5	42	theme	lipid	849:853	arg1	profiles					855:862	lipid profiles	849:862	lipid profiles	849:862	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	7	43	theme	HF-induced	1153:1162	arg1	increase					1164:1171	the HF-induced increase	1149:1171	the HF-induced increase in fat mass and energy intake	1149:1201	2'-FL significantly attenuated the HF-induced increase in fat mass and energy intake.					
34308934	2	44	theme	-fed	390:393	arg1	mice					395:398	high-fat (HF)-fed mice	377:398	high-fat (HF)-fed mice	377:398	It has previously been shown that 2'-FL can improve the metabolic phenotype in high-fat (HF)-fed mice.					
34308934	4	45	theme	w/w	718:720	arg1	%					716:716	10% w/w	714:720	10% w/w	714:720	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	4	45	theme	w/w	718:720	arg1	2'-FL					707:711	2'-FL	707:711	2'-FL (10% w/w)	707:721	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	10	46	from	effect	1562:1567	arg1	function					1582:1589	cognitive function	1572:1589	cognitive function	1572:1589	Signaling in the vagal afferent pathway was improved but there was no effect on cognitive function.					
34308934	4	47	theme	low-fat	611:617	arg1	diet					667:670	either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet	602:670	diet	667:670	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	2	48	theme	metabolic	354:362	arg1	phenotype					364:372	the metabolic phenotype	350:372	the metabolic phenotype in high-fat (HF)-fed mice	350:398	It has previously been shown that 2'-FL can improve the metabolic phenotype in high-fat (HF)-fed mice.					
34308934	1	49	theme	well-established	249:264	arg1	effects					277:283	several well-established beneficial effects	241:283	several well-established beneficial effects in the host	241:295	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	10	50	from	Signaling	1492:1500	arg1	pathway					1524:1530	the vagal afferent pathway	1505:1530	the vagal afferent pathway	1505:1530	Signaling in the vagal afferent pathway was improved but there was no effect on cognitive function.					
34308934	9	51	from	changes	1405:1411	arg1	composition					1435:1445	the gut microbiota composition	1416:1445	the gut microbiota composition	1416:1445	Additionally, 2'-FL led to changes in the gut microbiota composition and in the associated microbial metabolites.					
34308934	9	51	from	changes	1405:1411	arg1	metabolites					1479:1489	the associated microbial metabolites	1454:1489	the associated microbial metabolites	1454:1489	Additionally, 2'-FL led to changes in the gut microbiota composition and in the associated microbial metabolites.					
34308934	6	52	theme	Vagal	946:950	arg1	activity					961:968	Vagal afferent activity	946:968	Vagal afferent activity	946:968	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	3	53	from	integrity	513:521	arg1	pathway					556:562	the vagal afferent pathway	537:562	the vagal afferent pathway	537:562	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	3	54	theme	vagal	541:545	arg1	pathway					556:562	the vagal afferent pathway	537:562	the vagal afferent pathway	537:562	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	3	55	from	signaling	524:532	arg1	pathway					556:562	the vagal afferent pathway	537:562	the vagal afferent pathway	537:562	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	5	56	theme	Body	748:751	arg1	weight					753:758	Body weight	748:758	Body weight	748:758	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	3	57	theme	cognitive	568:576	arg1	function					578:585	cognitive function	568:585	cognitive function	568:585	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	12	58	theme	homeostasis	1844:1854	arg1	control					1798:1804	the control	1794:1804	the control of gut barrier function and metabolic homeostasis	1794:1854	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	7	59	from	increase	1164:1171	arg1	mass					1180:1183	fat mass	1176:1183	fat mass	1176:1183	2'-FL significantly attenuated the HF-induced increase in fat mass and energy intake.					
34308934	7	59	from	increase	1164:1171	arg1	intake					1196:1201	energy intake	1189:1201	energy intake	1189:1201	2'-FL significantly attenuated the HF-induced increase in fat mass and energy intake.					
34308934	6	60	theme	protein	1026:1032	arg1	detection					1007:1015	immunohistochemical detection	987:1015	immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK)	987:1115	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	1	61	from	effects	277:283	arg1	host					292:295	the host	288:295	the host	288:295	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	1	62	theme	oligosaccharides	196:211	arg1	one					173:175	one	173:175	one	173:175	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	1	62	theme	oligosaccharides	196:211	arg1	oligosaccharides					196:211	the predominant oligosaccharides	180:211	the predominant oligosaccharides found in human milk	180:231	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
34308934	3	63	theme	barrier	505:511	arg1	integrity					513:521	improved intestinal barrier integrity	485:521	improved intestinal barrier integrity	485:521	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	5	64	theme	energy	761:766	arg1	intake					768:773	energy intake	761:773	energy intake	761:773	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	12	65	theme	barrier	1813:1819	arg1	function					1821:1828	gut barrier function	1809:1828	gut barrier function	1809:1828	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	8	66	theme	protective	1344:1353	arg1	role					1355:1358	its protective role	1340:1358	its protective role in the intestine	1340:1375	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	4	67	theme	10	624:625	arg1	%					626:626	%	626:626	%	626:626	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	5	68	theme	lean	784:787	arg1	mass					789:792	lean mass	784:792	lean mass	784:792	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	3	69	with	supplementation	438:452	arg1	2'-FL					459:463	2'-FL	459:463	2'-FL	459:463	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	0	70	from	pathway	127:133	arg1	mice					138:141	mice	138:141	mice	138:141	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	6	71	theme	cholecystokinin	1095:1109	arg1	administration					1077:1090	peripheral administration	1066:1090	peripheral administration of cholecystokinin (CCK)	1066:1115	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	4	72	dep	low-fat	611:617	arg1	LF					620:621	LF	620:621	LF	620:621	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	4	72	dep	low-fat	611:617	arg1	fat					628:630	10% fat	624:630	10% fat per kcal	624:639	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	11	73	theme	metabolic	1642:1650	arg1	signaling					1706:1714	signaling	1706:1714	signaling	1706:1714	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	11	73	theme	metabolic	1642:1650	arg1	integrity					1674:1682	gut barrier integrity	1662:1682	gut barrier integrity	1662:1682	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	11	73	theme	metabolic	1642:1650	arg1	metabolism					1691:1700	lipid metabolism	1685:1700	lipid metabolism	1685:1700	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	11	73	theme	metabolic	1642:1650	arg1	profiles					1652:1659	the metabolic profiles	1638:1659	the metabolic profiles	1638:1659	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	5	74	theme	cognitive	911:919	arg1	functions					921:929	cognitive functions	911:929	cognitive functions	911:929	Body weight, energy intake, fat and lean mass, intestinal permeability (ex vivo in Ussing chambers), lipid profiles, gut microbiome and microbial metabolites, and cognitive functions were measured.					
34308934	11	75	theme	barrier	1666:1672	arg1	integrity					1674:1682	gut barrier integrity	1662:1682	gut barrier integrity	1662:1682	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	11	75	theme	barrier	1666:1672	arg1	profiles					1652:1659	the metabolic profiles	1638:1659	the metabolic profiles	1638:1659	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	0	76	theme	vagal	112:116	arg1	pathway					127:133	the vagal afferent pathway	108:133	the vagal afferent pathway in mice	108:141	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	12	77	theme	gut	1809:1811	arg1	function					1821:1828	gut barrier function	1809:1828	gut barrier function	1809:1828	These findings support the utility of 2'-FL in the control of gut barrier function and metabolic homeostasis under a metabolic challenge.					
34308934	9	78	theme	gut	1420:1422	arg1	composition					1435:1445	the gut microbiota composition	1416:1445	the gut microbiota composition	1416:1445	Additionally, 2'-FL led to changes in the gut microbiota composition and in the associated microbial metabolites.					
34308934	6	79	from	brainstem	1041:1049	arg1	response					1054:1061	response	1054:1061	response to peripheral administration of cholecystokinin (CCK)	1054:1115	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	9	80	theme	microbiota	1424:1433	arg1	composition					1435:1445	the gut microbiota composition	1416:1445	the gut microbiota composition	1416:1445	Additionally, 2'-FL led to changes in the gut microbiota composition and in the associated microbial metabolites.					
34308934	10	81	theme	afferent	1515:1522	arg1	pathway					1524:1530	the vagal afferent pathway	1505:1530	the vagal afferent pathway	1505:1530	Signaling in the vagal afferent pathway was improved but there was no effect on cognitive function.					
34308934	3	82	theme	dietary	430:436	arg1	supplementation					438:452	dietary supplementation	430:452	dietary supplementation with 2'-FL	430:463	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	0	83	theme	milk	6:9	arg1	supplementation					45:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	2	84	theme	high-fat	377:384	arg1	mice					395:398	high-fat (HF)-fed mice	377:398	high-fat (HF)-fed mice	377:398	It has previously been shown that 2'-FL can improve the metabolic phenotype in high-fat (HF)-fed mice.					
34308934	0	85	theme	2'-fucosyllactose	27:43	arg1	supplementation					45:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation	0:59	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	4	86	dep	HF	645:646	arg1	%					651:651	45% fat	649:655	45% fat per kcal	649:664	Mice were fed either a low-fat (LF, 10% fat per kcal) or HF (45% fat per kcal) diet with or without supplementation of 2'-FL (10% w/w) in the diet for 8 weeks.					
34308934	9	87	theme	microbial	1469:1477	arg1	metabolites					1479:1489	the associated microbial metabolites	1454:1489	the associated microbial metabolites	1454:1489	Additionally, 2'-FL led to changes in the gut microbiota composition and in the associated microbial metabolites.					
34308934	7	88	theme	fat	1176:1178	arg1	mass					1180:1183	fat mass	1176:1183	fat mass	1176:1183	2'-FL significantly attenuated the HF-induced increase in fat mass and energy intake.					
34308934	8	89	theme	interleukin	1298:1308	arg1	IL					1311:1312	interleukin (IL)-22	1298:1316	interleukin (IL)-22	1298:1316	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	8	89	theme	interleukin	1298:1308	arg1	cytokine					1321:1328	a cytokine	1319:1328	a cytokine known for its protective role in the intestine	1319:1375	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	8	90	from	role	1355:1358	arg1	intestine					1367:1375	the intestine	1363:1375	the intestine	1363:1375	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	0	91	theme	barrier	74:80	arg1	function					82:89	gut barrier function	70:89	gut barrier function	70:89	Human milk oligosaccharide 2'-fucosyllactose supplementation improves gut barrier function and signaling in the vagal afferent pathway in mice.					
34308934	8	92	theme	IL	1311:1312	arg1	permeability					1243:1254	intestinal permeability	1232:1254	intestinal permeability	1232:1254	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	8	92	theme	IL	1311:1312	arg1	expression					1284:1293	significantly increased expression	1260:1293	significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine	1260:1375	2'-FL significantly reduced intestinal permeability and significantly increased expression of interleukin (IL)-22, a cytokine known for its protective role in the intestine.					
34308934	6	93	from	detection	1007:1015	arg1	brainstem					1041:1049	the brainstem	1037:1049	the brainstem in response to peripheral administration of cholecystokinin (CCK)	1037:1115	Vagal afferent activity was measured via immunohistochemical detection of c-Fos protein in the brainstem in response to peripheral administration of cholecystokinin (CCK).					
34308934	3	94	theme	intestinal	494:503	arg1	integrity					513:521	improved intestinal barrier integrity	485:521	improved intestinal barrier integrity	485:521	Here we investigated whether dietary supplementation with 2'-FL was associated with improved intestinal barrier integrity, signaling in the vagal afferent pathway and cognitive function.					
34308934	11	95	theme	afferent	1729:1736	arg1	pathway					1738:1744	the vagal afferent pathway	1719:1744	the vagal afferent pathway	1719:1744	In conclusion, 2'-FL supplementation improved the metabolic profiles, gut barrier integrity, lipid metabolism and signaling in the vagal afferent pathway.					
34308934	1	96	theme	several	241:247	arg1	effects					277:283	several well-established beneficial effects	241:283	several well-established beneficial effects in the host	241:295	2'-Fucosyllactose (2'-FL) is one of the predominant oligosaccharides found in human milk and has several well-established beneficial effects in the host.					
32429191	0	0	theme	FDM	73:75	arg1	Printing					80:87	FDM 3D Printing	73:87	FDM 3D Printing	73:87	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	8	1	theme	high-value	1101:1110	arg1	utilization					1112:1122	the high-value utilization	1097:1122	the high-value utilization of CNF in 3DP in consumer product applications	1097:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	8	2	theme	consumer	1141:1148	arg1	applications					1158:1169	consumer product applications	1141:1169	consumer product applications	1141:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	8	3	from	3DP	1134:1136	arg1	applications					1158:1169	consumer product applications	1141:1169	consumer product applications	1141:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	6	4	theme	thermal	794:800	arg1	stability					802:810	the thermal stability	790:810	the thermal stability of the PLA/PEG600/CNF composite	790:842	Findings showed that CNF increased the thermal stability of the PLA/PEG600/CNF composite.					
32429191	1	5	theme	3DP	136:138	arg1	technology					141:150	3D printing (3DP) technology	123:150	3D printing (3DP) technology	123:150	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	7	6	theme	unfilled	857:864	arg1	filaments					874:882	unfilled PLA FDM filaments	857:882	unfilled PLA FDM filaments	857:882	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	5	7	theme	Thermal	618:624	arg1	stability					626:634	Thermal stability	618:634	Thermal stability	618:634	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	7	8	theme	PLA	866:868	arg1	filaments					874:882	unfilled PLA FDM filaments	857:882	unfilled PLA FDM filaments	857:882	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	1	9	theme	wide	242:245	arg1	range					247:251	a wide range	240:251	a wide range of biological materials	240:275	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	0	10	theme	3D	77:78	arg1	Printing					80:87	FDM 3D Printing	73:87	FDM 3D Printing	73:87	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	1	11	theme	range	247:251	arg1	utilization					225:235	the high-value utilization	210:235	the high-value utilization of a wide range of biological materials	210:275	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	5	12	theme	filaments	707:715	arg1	performance					648:658	mechanical performance	637:658	mechanical performance	637:658	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	5	12	theme	filaments	707:715	arg1	property					682:689	water absorption property	665:689	water absorption property	665:689	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	5	12	theme	filaments	707:715	arg1	stability					626:634	Thermal stability	618:634	Thermal stability	618:634	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	8	13	theme	CNF	1127:1129	arg1	utilization					1112:1122	the high-value utilization	1097:1122	the high-value utilization of CNF in 3DP in consumer product applications	1097:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	8	14	theme	new	1079:1081	arg1	potential					1083:1091	a new potential	1077:1091	a new potential for the high-value utilization of CNF in 3DP in consumer product applications	1077:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	2	15	theme	deposition	365:374	arg1	3DP					391:393	fused deposition modeling (FDM) 3DP	359:393	fused deposition modeling (FDM) 3DP	359:393	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	6	16	theme	composite	834:842	arg1	stability					802:810	the thermal stability	790:810	the thermal stability of the PLA/PEG600/CNF composite	790:842	Findings showed that CNF increased the thermal stability of the PLA/PEG600/CNF composite.					
32429191	3	17	theme	high-pressure	489:501	arg1	homogenization					503:516	high-pressure homogenization	489:516	high-pressure homogenization	489:516	Firstly, CNF was isolated by enzymatic hydrolysis combined with high-pressure homogenization.					
32429191	2	18	theme	fused	359:363	arg1	3DP					391:393	fused deposition modeling (FDM) 3DP	359:393	fused deposition modeling (FDM) 3DP	359:393	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	7	19	theme	desktop	1040:1046	arg1	3DP					1052:1054	desktop FDM 3DP	1040:1054	desktop FDM 3DP	1040:1054	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	0	20	theme	Cellulose	0:8	arg1	Nanofibrils					10:20	Cellulose Nanofibrils	0:20	Cellulose Nanofibrils	0:20	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	7	21	theme	FDM	1048:1050	arg1	3DP					1052:1054	desktop FDM 3DP	1040:1054	desktop FDM 3DP	1040:1054	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	22	theme	PLA	900:902	arg1	filament					917:924	the CNF filled PLA biocomposite filament	885:924	the CNF filled PLA biocomposite filament	885:924	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	6	23	theme	PLA/PEG600/CNF	819:832	arg1	composite					834:842	the PLA/PEG600/CNF composite	815:842	the PLA/PEG600/CNF composite	815:842	Findings showed that CNF increased the thermal stability of the PLA/PEG600/CNF composite.					
32429191	1	24	theme	new	161:163	arg1	directions					177:186	new development directions and opportunities	161:204	directions	177:186	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	0	25	theme	Poly	29:32	arg1	Filament					60:67	Poly(Lactic Acid) Biocomposite Filament	29:67	Poly(Lactic Acid) Biocomposite Filament	29:67	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	1	26	theme	biological	256:265	arg1	materials					267:275	biological materials	256:275	biological materials	256:275	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	7	27	theme	biocomposite	904:915	arg1	filament					917:924	the CNF filled PLA biocomposite filament	885:924	the CNF filled PLA biocomposite filament	885:924	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	1	28	theme	development	165:175	arg1	directions					177:186	new development directions and opportunities	161:204	directions	177:186	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	1	29	theme	materials	267:275	arg1	range					247:251	a wide range	240:251	a wide range of biological materials	240:275	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	5	30	theme	water	665:669	arg1	property					682:689	water absorption property	665:689	water absorption property	665:689	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	2	31	theme	Cellulose	278:286	arg1	nanofibrils					288:298	Cellulose nanofibrils	278:298	Cellulose nanofibrils (CNF)	278:304	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	2	31	theme	Cellulose	278:286	arg1	CNF					301:303	CNF	301:303	CNF	301:303	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	2	32	theme	FDM	386:388	arg1	3DP					391:393	fused deposition modeling (FDM) 3DP	359:393	fused deposition modeling (FDM) 3DP	359:393	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	0	33	theme	Lactic	34:39	arg1	Poly					29:32	Poly	29:32	Poly(Lactic Acid) Biocomposite Filament	29:67	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	0	33	theme	Lactic	34:39	arg1	Acid					41:44	Lactic Acid	34:44	Lactic Acid	34:44	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	2	34	theme	polylactic	310:319	arg1	PLA					327:329	PLA	327:329	PLA	327:329	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	2	34	theme	polylactic	310:319	arg1	acid					321:324	polylactic acid	310:324	polylactic acid (PLA)	310:330	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	5	35	theme	absorption	671:680	arg1	property					682:689	water absorption property	665:689	water absorption property	665:689	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	7	36	from	strength	963:970	arg1	increase					936:943	an increase	933:943	an increase of 33% in tensile strength and 19% in elongation at break	933:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	37	from	%	978:978	arg1	break					997:1001	break	997:1001	break	997:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	37	from	%	978:978	arg1	elongation					983:992	elongation	983:992	elongation at break	983:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	37	from	%	978:978	arg1	strength					963:970	tensile strength	955:970	tensile strength	955:970	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	38	from	%	950:950	arg1	break					997:1001	break	997:1001	break	997:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	38	from	%	950:950	arg1	elongation					983:992	elongation	983:992	elongation at break	983:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	38	from	%	950:950	arg1	strength					963:970	tensile strength	955:970	tensile strength	955:970	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	39	theme	better	1015:1020	arg1	compatibility					1022:1034	better compatibility	1015:1034	better compatibility for desktop FDM 3DP	1015:1054	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	40	from	break	997:1001	arg1	%					950:950	33%	948:950	33% in tensile strength	948:970	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	40	from	break	997:1001	arg1	%					978:978	19%	976:978	19% in elongation at break	976:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	40	from	break	997:1001	arg1	elongation					983:992	elongation	983:992	elongation at break	983:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	41	theme	%	978:978	arg1	increase					936:943	an increase	933:943	an increase of 33% in tensile strength and 19% in elongation at break	933:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	2	42	theme	modeling	376:383	arg1	3DP					391:393	fused deposition modeling (FDM) 3DP	359:393	fused deposition modeling (FDM) 3DP	359:393	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	8	43	theme	product	1150:1156	arg1	applications					1158:1169	consumer product applications	1141:1169	consumer product applications	1141:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	4	44	theme	CNF/PLA	519:525	arg1	filaments					527:535	CNF/PLA filaments	519:535	CNF/PLA filaments	519:535	CNF/PLA filaments were then prepared by melt-extrusion of PLA as the matrix and CNF as the filler.					
32429191	4	44	theme	CNF/PLA	519:525	arg1	CNF					599:601	CNF	599:601	CNF	599:601	CNF/PLA filaments were then prepared by melt-extrusion of PLA as the matrix and CNF as the filler.					
32429191	4	44	theme	CNF/PLA	519:525	arg1	matrix					588:593	matrix	588:593	matrix	588:593	CNF/PLA filaments were then prepared by melt-extrusion of PLA as the matrix and CNF as the filler.					
32429191	1	45	theme	direct	93:98	arg1	manufacturing					108:120	direct digital manufacturing	93:120	direct digital manufacturing	93:120	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	5	46	theme	mechanical	637:646	arg1	performance					648:658	mechanical performance	637:658	mechanical performance	637:658	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	4	47	dep	matrix	588:593	arg1	the					584:586	the	584:586	the	584:586	CNF/PLA filaments were then prepared by melt-extrusion of PLA as the matrix and CNF as the filler.					
32429191	3	48	theme	enzymatic	454:462	arg1	hydrolysis					464:473	enzymatic hydrolysis	454:473	enzymatic hydrolysis combined with high-pressure homogenization	454:516	Firstly, CNF was isolated by enzymatic hydrolysis combined with high-pressure homogenization.					
32429191	1	49	theme	digital	100:106	arg1	manufacturing					108:120	direct digital manufacturing	93:120	direct digital manufacturing	93:120	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	5	50	theme	objects	732:738	arg1	performance					648:658	mechanical performance	637:658	mechanical performance	637:658	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	5	50	theme	objects	732:738	arg1	property					682:689	water absorption property	665:689	water absorption property	665:689	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	5	50	theme	objects	732:738	arg1	stability					626:634	Thermal stability	618:634	Thermal stability	618:634	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	7	51	theme	filled	893:898	arg1	filament					917:924	the CNF filled PLA biocomposite filament	885:924	the CNF filled PLA biocomposite filament	885:924	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	52	from	elongation	983:992	arg1	increase					936:943	an increase	933:943	an increase of 33% in tensile strength and 19% in elongation at break	933:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	8	53	from	utilization	1112:1122	arg1	3DP					1134:1136	3DP	1134:1136	3DP in consumer product applications	1134:1169	This study provided a new potential for the high-value utilization of CNF in 3DP in consumer product applications.					
32429191	2	54	dep	filaments	345:353	arg1	developed					400:408	developed	400:408	were developed in this study	395:422	Cellulose nanofibrils (CNF) and polylactic acid (PLA) biocomposite filaments for fused deposition modeling (FDM) 3DP were developed in this study.					
32429191	7	55	theme	tensile	955:961	arg1	strength					963:970	tensile strength	955:970	tensile strength	955:970	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	56	from	increase	936:943	arg1	elongation					983:992	elongation	983:992	elongation at break	983:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	7	56	from	increase	936:943	arg1	strength					963:970	tensile strength	955:970	tensile strength	955:970	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	4	57	theme	PLA	577:579	arg1	melt-extrusion					559:572	melt-extrusion	559:572	melt-extrusion of PLA	559:579	CNF/PLA filaments were then prepared by melt-extrusion of PLA as the matrix and CNF as the filler.					
32429191	5	58	theme	3D-printed	721:730	arg1	objects					732:738	3D-printed objects	721:738	3D-printed objects	721:738	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32429191	1	59	theme	3D	123:124	arg1	technology					141:150	3D printing (3DP) technology	123:150	3D printing (3DP) technology	123:150	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	1	60	theme	high-value	214:223	arg1	utilization					225:235	the high-value utilization	210:235	the high-value utilization of a wide range of biological materials	210:275	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	0	61	theme	Biocomposite	47:58	arg1	Filament					60:67	Poly(Lactic Acid) Biocomposite Filament	29:67	Poly(Lactic Acid) Biocomposite Filament	29:67	Cellulose Nanofibrils Filled Poly(Lactic Acid) Biocomposite Filament for FDM 3D Printing.					
32429191	7	62	theme	%	950:950	arg1	increase					936:943	an increase	933:943	an increase of 33% in tensile strength and 19% in elongation at break	933:1001	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	1	63	theme	printing	126:133	arg1	technology					141:150	3D printing (3DP) technology	123:150	3D printing (3DP) technology	123:150	As direct digital manufacturing, 3D printing (3DP) technology provides new development directions and opportunities for the high-value utilization of a wide range of biological materials.					
32429191	7	64	theme	FDM	870:872	arg1	filaments					874:882	unfilled PLA FDM filaments	857:882	unfilled PLA FDM filaments	857:882	Compared to unfilled PLA FDM filaments, the CNF filled PLA biocomposite filament showed an increase of 33% in tensile strength and 19% in elongation at break, suggesting better compatibility for desktop FDM 3DP.					
32429191	5	65	theme	biocomposite	694:705	arg1	filaments					707:715	biocomposite filaments	694:715	biocomposite filaments	694:715	Thermal stability, mechanical performance, and water absorption property of biocomposite filaments and 3D-printed objects were analyzed.					
32339572	1	0	theme	electrospun	241:251	arg1	FCNLs					284:288	FCNLs	284:288	FCNLs	284:288	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	0	theme	electrospun	241:251	arg1	nano-layers					271:281	electrospun fibrous composite nano-layers	241:281	electrospun fibrous composite nano-layers (FCNLs)	241:289	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	2	1	with	combination	367:377	arg1	AgNPs					388:392	the AgNPs	384:392	the AgNPs which were characterised using UV-visible spectrophotometry	384:452	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	1	2	theme	fibrous	253:259	arg1	FCNLs					284:288	FCNLs	284:288	FCNLs	284:288	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	2	theme	fibrous	253:259	arg1	nano-layers					271:281	electrospun fibrous composite nano-layers	241:281	electrospun fibrous composite nano-layers (FCNLs)	241:289	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	3	theme	Stable	85:90	arg1	AgNPs					114:118	AgNPs	114:118	AgNPs	114:118	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	3	theme	Stable	85:90	arg1	nanoparticles					99:111	Stable silver nanoparticles	85:111	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis	85:189	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	4	theme	composite	261:269	arg1	FCNLs					284:288	FCNLs	284:288	FCNLs	284:288	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	4	theme	composite	261:269	arg1	nano-layers					271:281	electrospun fibrous composite nano-layers	241:281	electrospun fibrous composite nano-layers (FCNLs)	241:289	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	2	5	theme	electron	499:506	arg1	stabilising					318:328	stabilising	318:328	stabilising	318:328	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	5	theme	electron	499:506	arg1	chitosan					296:303	The chitosan	292:303	The chitosan	292:303	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	5	theme	electron	499:506	arg1	SEM					520:522	SEM	520:522	SEM	520:522	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	5	theme	electron	499:506	arg1	microscopy					508:517	scanning electron microscopy	490:517	scanning electron microscopy (SEM)	490:523	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	5	theme	electron	499:506	arg1	scattering					469:478	dynamic light scattering	455:478	dynamic light scattering (DLS)	455:484	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	5	6	theme	composite	935:943	arg1	electrospun					923:933	The electrospun composite	919:943	The electrospun composite	919:943	The electrospun composite, when tested as packaging material for meat, showed bio-activity and extended the meat shelf-life by one week.					
32339572	6	7	theme	electrospun	1060:1070	arg1	able					1089:1092	able	1089:1092	able	1089:1092	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	6	7	theme	electrospun	1060:1070	arg1	nanocomposite					1072:1084	The electrospun nanocomposite	1056:1084	The electrospun nanocomposite	1056:1084	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	1	8	theme	silver	92:97	arg1	AgNPs					114:118	AgNPs	114:118	AgNPs	114:118	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	8	theme	silver	92:97	arg1	nanoparticles					99:111	Stable silver nanoparticles	85:111	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis	85:189	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	9	theme	mediated	166:173	arg1	synthesis					181:189	chitosan (CH) mediated green synthesis	152:189	chitosan (CH) mediated green synthesis	152:189	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	2	10	theme	scanning	490:497	arg1	stabilising					318:328	stabilising	318:328	stabilising	318:328	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	10	theme	scanning	490:497	arg1	chitosan					296:303	The chitosan	292:303	The chitosan	292:303	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	10	theme	scanning	490:497	arg1	SEM					520:522	SEM	520:522	SEM	520:522	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	10	theme	scanning	490:497	arg1	microscopy					508:517	scanning electron microscopy	490:517	scanning electron microscopy (SEM)	490:523	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	10	theme	scanning	490:497	arg1	scattering					469:478	dynamic light scattering	455:478	dynamic light scattering (DLS)	455:484	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	0	11	theme	Antimicrobial	0:12	arg1	biodegradable					14:26	Antimicrobial biodegradable	0:26	Antimicrobial biodegradable	0:26	Antimicrobial biodegradable chitosan-based composite Nano-layers for food packaging.					
32339572	1	12	theme	green	175:179	arg1	synthesis					181:189	chitosan (CH) mediated green synthesis	152:189	chitosan (CH) mediated green synthesis	152:189	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	3	13	theme	chemical	548:555	arg1	nature					557:562	chemical nature	548:562	chemical nature	548:562	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	6	14	from	quality	1167:1173	arg1	manner					1194:1199	an eco-friendly manner	1178:1199	an eco-friendly manner	1178:1199	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	2	15	theme	UV-visible	425:434	arg1	spectrophotometry					436:452	UV-visible spectrophotometry	425:452	UV-visible spectrophotometry	425:452	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	6	16	theme	eco-friendly	1181:1192	arg1	manner					1194:1199	an eco-friendly manner	1178:1199	an eco-friendly manner	1178:1199	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	4	17	theme	gram	858:861	arg1	monocytogenes					843:855	Listeria monocytogenes	834:855	Listeria monocytogenes (gram +ve)	834:866	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	4	17	theme	gram	858:861	arg1	+ve					863:865	gram +ve	858:865	gram +ve	858:865	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	5	18	theme	meat	1027:1030	arg1	shelf-life					1032:1041	the meat shelf-life	1023:1041	the meat shelf-life	1023:1041	The electrospun composite, when tested as packaging material for meat, showed bio-activity and extended the meat shelf-life by one week.					
32339572	0	19	theme	composite	43:51	arg1	Nano-layers					53:63	composite Nano-layers	43:63	composite Nano-layers for food packaging	43:82	Antimicrobial biodegradable chitosan-based composite Nano-layers for food packaging.					
32339572	3	20	theme	water	739:743	arg1	angle					753:757	the water contact angle	735:757	the water contact angle	735:757	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	6	21	theme	food	1139:1142	arg1	degradation					1115:1125	microbial degradation	1105:1125	microbial degradation of packaged food	1105:1142	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	4	22	dep	monocytogenes	843:855	arg1	species					910:916	bacterial species	900:916	bacterial species	900:916	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	4	23	theme	bacterial	900:908	arg1	species					910:916	bacterial species	900:916	bacterial species	900:916	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	6	24	theme	packaged	1130:1137	arg1	food					1139:1142	packaged food	1130:1142	packaged food	1130:1142	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	3	25	dep	crystallinity	530:542	arg1	The					526:528	The	526:528	The	526:528	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	3	26	theme	electrospun	571:581	arg1	composite					583:591	the electrospun composite	567:591	the electrospun composite	567:591	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	1	27	theme	polyvinyl	209:217	arg1	PVA					228:230	PVA	228:230	PVA	228:230	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	27	theme	polyvinyl	209:217	arg1	alcohol					219:225	polyvinyl alcohol	209:225	polyvinyl alcohol (PVA)	209:231	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	2	28	theme	light	463:467	arg1	stabilising					318:328	stabilising	318:328	stabilising	318:328	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	28	theme	light	463:467	arg1	microscopy					508:517	scanning electron microscopy	490:517	scanning electron microscopy (SEM)	490:523	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	28	theme	light	463:467	arg1	chitosan					296:303	The chitosan	292:303	The chitosan	292:303	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	28	theme	light	463:467	arg1	DLS					481:483	DLS	481:483	DLS	481:483	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	28	theme	light	463:467	arg1	scattering					469:478	dynamic light scattering	455:478	dynamic light scattering (DLS)	455:484	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	3	29	theme	X-ray	620:624	arg1	XRD					639:641	XRD	639:641	XRD	639:641	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	3	29	theme	X-ray	620:624	arg1	diffraction					626:636	X-ray diffraction	620:636	X-ray diffraction (XRD)	620:642	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	3	30	theme	composite	583:591	arg1	crystallinity					530:542	crystallinity	530:542	crystallinity	530:542	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	3	30	theme	composite	583:591	arg1	nature					557:562	chemical nature	548:562	chemical nature	548:562	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	2	31	theme	dynamic	455:461	arg1	stabilising					318:328	stabilising	318:328	stabilising	318:328	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	31	theme	dynamic	455:461	arg1	microscopy					508:517	scanning electron microscopy	490:517	scanning electron microscopy (SEM)	490:523	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	31	theme	dynamic	455:461	arg1	chitosan					296:303	The chitosan	292:303	The chitosan	292:303	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	31	theme	dynamic	455:461	arg1	DLS					481:483	DLS	481:483	DLS	481:483	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	2	31	theme	dynamic	455:461	arg1	scattering					469:478	dynamic light scattering	455:478	dynamic light scattering (DLS)	455:484	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	4	32	theme	gram	890:893	arg1	-ve					895:897	gram -ve	890:897	gram -ve	890:897	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	4	32	theme	gram	890:893	arg1	coli					884:887	Escherichia coli	872:887	Escherichia coli (gram -ve)	872:898	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	4	33	theme	Listeria	834:841	arg1	monocytogenes					843:855	Listeria monocytogenes	834:855	Listeria monocytogenes (gram +ve)	834:866	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	4	33	theme	Listeria	834:841	arg1	+ve					863:865	gram +ve	858:865	gram +ve	858:865	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	2	34	from	agent	358:362	arg1	combination					367:377	combination	367:377	combination with the AgNPs which were characterised using UV-visible spectrophotometry	367:452	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	1	35	theme	size	124:127	arg1	80 ± 11 nm					129:138	size 80 ± 11 nm	124:138	size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis	124:189	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	0	36	theme	food	69:72	arg1	packaging					74:82	food packaging	69:82	food packaging	69:82	Antimicrobial biodegradable chitosan-based composite Nano-layers for food packaging.					
32339572	3	37	theme	contact	745:751	arg1	angle					753:757	the water contact angle	735:757	the water contact angle	735:757	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	3	38	theme	FTIR	648:651	arg1	spectroscopy					653:664	FTIR spectroscopy	648:664	FTIR spectroscopy	648:664	The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle.					
32339572	1	39	theme	80 ± 11 nm	129:138	arg1	AgNPs					114:118	AgNPs	114:118	AgNPs	114:118	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	1	39	theme	80 ± 11 nm	129:138	arg1	nanoparticles					99:111	Stable silver nanoparticles	85:111	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis	85:189	Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs).					
32339572	4	40	theme	antimicrobial	803:815	arg1	activity					817:824	effective antimicrobial activity	793:824	effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species	793:916	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	6	41	theme	microbial	1105:1113	arg1	degradation					1115:1125	microbial degradation	1105:1125	microbial degradation of packaged food	1105:1142	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32339572	4	42	theme	electrospun	764:774	arg1	composite					776:784	The electrospun composite	760:784	The electrospun composite	760:784	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	5	43	theme	packaging	961:969	arg1	material					971:978	packaging material	961:978	packaging material	961:978	The electrospun composite, when tested as packaging material for meat, showed bio-activity and extended the meat shelf-life by one week.					
32339572	4	44	theme	effective	793:801	arg1	activity					817:824	effective antimicrobial activity	793:824	effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species	793:916	The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram -ve) bacterial species.					
32339572	2	45	theme	antimicrobial	344:356	arg1	agent					358:362	an antimicrobial agent	341:362	an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry	341:452	The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV-visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM).					
32339572	6	46	theme	keeping	1159:1165	arg1	quality					1167:1173	its keeping quality	1155:1173	its keeping quality in an eco-friendly manner	1155:1199	The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner.					
32738327	10	0	theme	GDSF	1001:1004	arg1	stability					988:996	The thermal stability	976:996	The thermal stability of GDSF	976:1004	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	8	1	theme	microscopy	779:788	arg1	results					796:802	The morphological and atomic force microscopy study results	744:802	The morphological and atomic force microscopy study results	744:802	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	7	2	theme	GDSF	641:644	arg1	properties					627:636	The mechanical properties	612:636	The mechanical properties of GDSF	612:644	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	2	theme	GDSF	641:644	arg1	modulus					721:727	126.2 ± 11.93 GPa modulus	703:727	126.2 ± 11.93 GPa modulus	703:727	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	2	theme	GDSF	641:644	arg1	strength					690:697	375.6 ± 16.58 MPa tensile strength	664:697	375.6 ± 16.58 MPa tensile strength	664:697	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	3	3	from	contents	336:343	arg1	rich					318:321	rich	318:321	rich	318:321	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	4	4	from	use	419:421	arg1	applications					438:449	lightweight applications	426:449	lightweight applications	426:449	Its lower density (1.378 ± 0.036 g/cm3) makes it fit for use in lightweight applications.					
32738327	5	5	theme	chemical	488:495	arg1	constituents					497:508	the chemical constituents	484:508	the chemical constituents	484:508	The weight percentage values of the chemical constituents confirm the use of GDSF for structural applications.					
32738327	10	6	theme	GDSF	1124:1127	arg1	values					1114:1119	the physical and tensile property values	1080:1119	the physical and tensile property values of GDSF	1080:1127	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	10	7	theme	Weibull	1027:1033	arg1	plots					1048:1052	326 °C. Weibull distribution plots	1019:1052	326 °C. Weibull distribution plots	1019:1052	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	3	8	theme	stem	291:294	arg1	bark					296:299	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	1	9	located	found	133:137	arg2	damine					105:110	Grewia damine	98:110	Grewia damine	98:110	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	1	9	located	found	133:137	arg2	plant					127:131	a flowering plant	115:131	a flowering plant found in India and Sri Lanka	115:160	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	1	9	located	found	133:137	arg1	India					142:146	India	142:146	India	142:146	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	1	9	located	found	133:137	arg1	Lanka					156:160	Sri Lanka	152:160	Sri Lanka	152:160	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	5	10	theme	GDSF	529:532	arg1	use					522:524	the use	518:524	the use of GDSF for structural applications	518:560	The weight percentage values of the chemical constituents confirm the use of GDSF for structural applications.					
32738327	10	11	theme	physical	1084:1091	arg1	values					1114:1119	the physical and tensile property values	1080:1119	the physical and tensile property values of GDSF	1080:1127	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	1	12	theme	Sri	152:154	arg1	Lanka					156:160	Sri Lanka	152:160	Sri Lanka	152:160	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	9	13	theme	bonding	932:938	arg1	strength					940:947	better bonding strength	925:947	better bonding strength between fiber and polymer	925:973	Hence it can be used to prepare composite specimens with better bonding strength between fiber and polymer.					
32738327	5	14	theme	structural	538:547	arg1	applications					549:560	structural applications	538:560	structural applications	538:560	The weight percentage values of the chemical constituents confirm the use of GDSF for structural applications.					
32738327	10	15	theme	property	1105:1112	arg1	values					1114:1119	the physical and tensile property values	1080:1119	the physical and tensile property values of GDSF	1080:1127	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	9	16	theme	composite	900:908	arg1	specimens					910:918	composite specimens	900:918	composite specimens	900:918	Hence it can be used to prepare composite specimens with better bonding strength between fiber and polymer.					
32738327	3	17	from	rich	318:321	arg1	contents					336:343	cellulose contents	326:343	cellulose contents (57.78 ± 3.56%)	326:359	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	3	17	from	rich	318:321	arg1	%					358:358	57.78 ± 3.56%	346:358	57.78 ± 3.56%	346:358	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	0	18	theme	flowering	74:82	arg1	stem					92:95	Grewia damine flowering plant's stem	60:95	Grewia damine flowering plant's stem	60:95	Characterization of natural cellulosic fiber extracted from Grewia damine flowering plant's stem.					
32738327	8	19	contain	has	828:830	arg1	fiber					822:826	the fiber	818:826	the fiber	818:826	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	8	19	contain	has	828:830	arg2	surface					838:844	rough surface	832:844	rough surface	832:844	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	10	20	theme	326 °C.	1019:1025	arg1	plots					1048:1052	326 °C. Weibull distribution plots	1019:1052	326 °C. Weibull distribution plots	1019:1052	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	4	21	dep	it	408:409	arg1	fit					411:413	fit	411:413	fit for use in lightweight applications	411:449	Its lower density (1.378 ± 0.036 g/cm3) makes it fit for use in lightweight applications.					
32738327	5	22	theme	constituents	497:508	arg1	values					474:479	The weight percentage values	452:479	The weight percentage values of the chemical constituents	452:508	The weight percentage values of the chemical constituents confirm the use of GDSF for structural applications.					
32738327	0	23	theme	cellulosic	28:37	arg1	fiber					39:43	natural cellulosic fiber	20:43	natural cellulosic fiber	20:43	Characterization of natural cellulosic fiber extracted from Grewia damine flowering plant's stem.					
32738327	1	24	theme	Grewia	98:103	arg1	plant					127:131	a flowering plant	115:131	a flowering plant found in India and Sri Lanka	115:160	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	1	24	theme	Grewia	98:103	arg1	damine					105:110	Grewia damine	98:110	Grewia damine	98:110	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	8	25	theme	rough	832:836	arg1	surface					838:844	rough surface	832:844	rough surface	832:844	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	0	26	theme	natural	20:26	arg1	fiber					39:43	natural cellulosic fiber	20:43	natural cellulosic fiber	20:43	Characterization of natural cellulosic fiber extracted from Grewia damine flowering plant's stem.					
32738327	10	27	theme	distribution	1035:1046	arg1	plots					1048:1052	326 °C. Weibull distribution plots	1019:1052	326 °C. Weibull distribution plots	1019:1052	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	4	28	theme	lower	366:370	arg1	1.378 ± 0.036 g/cm3					381:399	1.378 ± 0.036 g/cm3	381:399	1.378 ± 0.036 g/cm3	381:399	Its lower density (1.378 ± 0.036 g/cm3) makes it fit for use in lightweight applications.					
32738327	4	28	theme	lower	366:370	arg1	density					372:378	Its lower density	362:378	Its lower density (1.378 ± 0.036 g/cm3)	362:400	Its lower density (1.378 ± 0.036 g/cm3) makes it fit for use in lightweight applications.					
32738327	7	29	theme	elasticity	732:741	arg1	strength					690:697	375.6 ± 16.58 MPa tensile strength	664:697	375.6 ± 16.58 MPa tensile strength	664:697	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	29	theme	elasticity	732:741	arg1	modulus					721:727	126.2 ± 11.93 GPa modulus	703:727	126.2 ± 11.93 GPa modulus	703:727	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	29	theme	elasticity	732:741	arg1	properties					627:636	The mechanical properties	612:636	The mechanical properties of GDSF	612:644	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	30	theme	mechanical	616:625	arg1	properties					627:636	The mechanical properties	612:636	The mechanical properties of GDSF	612:644	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	30	theme	mechanical	616:625	arg1	modulus					721:727	126.2 ± 11.93 GPa modulus	703:727	126.2 ± 11.93 GPa modulus	703:727	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	30	theme	mechanical	616:625	arg1	strength					690:697	375.6 ± 16.58 MPa tensile strength	664:697	375.6 ± 16.58 MPa tensile strength	664:697	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	8	31	theme	atomic	766:771	arg1	microscopy					779:788	atomic force microscopy	766:788	atomic force microscopy	766:788	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	0	32	theme	fiber	39:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of natural cellulosic fiber	0:43	Characterization of natural cellulosic fiber extracted from Grewia damine flowering plant's stem.					
32738327	7	33	theme	126.2 ± 11.93 GPa	703:719	arg1	properties					627:636	The mechanical properties	612:636	The mechanical properties of GDSF	612:644	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	33	theme	126.2 ± 11.93 GPa	703:719	arg1	modulus					721:727	126.2 ± 11.93 GPa modulus	703:727	126.2 ± 11.93 GPa modulus	703:727	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	10	34	theme	tensile	1097:1103	arg1	values					1114:1119	the physical and tensile property values	1080:1119	the physical and tensile property values of GDSF	1080:1127	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	5	35	theme	weight	456:461	arg1	values					474:479	The weight percentage values	452:479	The weight percentage values of the chemical constituents	452:508	The weight percentage values of the chemical constituents confirm the use of GDSF for structural applications.					
32738327	0	36	theme	Grewia	60:65	arg1	stem					92:95	Grewia damine flowering plant's stem	60:95	Grewia damine flowering plant's stem	60:95	Characterization of natural cellulosic fiber extracted from Grewia damine flowering plant's stem.					
32738327	4	37	theme	lightweight	426:436	arg1	applications					438:449	lightweight applications	426:449	lightweight applications	426:449	Its lower density (1.378 ± 0.036 g/cm3) makes it fit for use in lightweight applications.					
32738327	0	38	theme	damine	67:72	arg1	stem					92:95	Grewia damine flowering plant's stem	60:95	Grewia damine flowering plant's stem	60:95	Characterization of natural cellulosic fiber extracted from Grewia damine flowering plant's stem.					
32738327	2	39	theme	stem	192:195	arg1	bark					197:200	the stem bark	188:200	the stem bark of G. damine	188:213	The fiber taken out from the stem bark of G. damine is found to be novel and selected for characterization study.					
32738327	5	40	theme	percentage	463:472	arg1	values					474:479	The weight percentage values	452:479	The weight percentage values of the chemical constituents	452:508	The weight percentage values of the chemical constituents confirm the use of GDSF for structural applications.					
32738327	3	41	theme	cellulose	326:334	arg1	contents					336:343	cellulose contents	326:343	cellulose contents (57.78 ± 3.56%)	326:359	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	3	41	theme	cellulose	326:334	arg1	%					358:358	57.78 ± 3.56%	346:358	57.78 ± 3.56%	346:358	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	3	42	dep	bark	296:299	arg1	GDSF					308:311	GDSF	308:311	GDSF	308:311	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	3	42	dep	bark	296:299	arg1	fiber					301:305	fiber	301:305	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	10	43	theme	thermal	980:986	arg1	stability					988:996	The thermal stability	976:996	The thermal stability of GDSF	976:1004	The thermal stability of GDSF was noted as 326 °C. Weibull distribution plots were drawn for validating the physical and tensile property values of GDSF.					
32738327	6	44	theme	GDSF	586:589	arg1	index					577:581	Crystallinity index	563:581	Crystallinity index of GDSF	563:589	Crystallinity index of GDSF was noted as 30.35%.					
32738327	6	44	theme	GDSF	586:589	arg1	%					609:609	30.35%	604:609	30.35%	604:609	Crystallinity index of GDSF was noted as 30.35%.					
32738327	9	45	used	used	884:887	arg2	it					874:875	it	874:875	it	874:875	Hence it can be used to prepare composite specimens with better bonding strength between fiber and polymer.					
32738327	1	46	theme	flowering	117:125	arg1	plant					127:131	a flowering plant	115:131	a flowering plant found in India and Sri Lanka	115:160	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	1	46	theme	flowering	117:125	arg1	damine					105:110	Grewia damine	98:110	Grewia damine	98:110	Grewia damine is a flowering plant found in India and Sri Lanka.					
32738327	8	47	theme	morphological	748:760	arg1	results					796:802	The morphological and atomic force microscopy study results	744:802	The morphological and atomic force microscopy study results	744:802	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	7	48	theme	375.6 ± 16.58 MPa	664:680	arg1	strength					690:697	375.6 ± 16.58 MPa tensile strength	664:697	375.6 ± 16.58 MPa tensile strength	664:697	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	48	theme	375.6 ± 16.58 MPa	664:680	arg1	properties					627:636	The mechanical properties	612:636	The mechanical properties of GDSF	612:644	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	8	49	theme	raw	858:860	arg1	form					862:865	its raw form	854:865	its raw form	854:865	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	2	50	dep	characterization	253:268	arg1	study					270:274	study	270:274	study	270:274	The fiber taken out from the stem bark of G. damine is found to be novel and selected for characterization study.					
32738327	8	51	theme	force	773:777	arg1	microscopy					779:788	atomic force microscopy	766:788	atomic force microscopy	766:788	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32738327	7	52	theme	tensile	682:688	arg1	strength					690:697	375.6 ± 16.58 MPa tensile strength	664:697	375.6 ± 16.58 MPa tensile strength	664:697	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	7	52	theme	tensile	682:688	arg1	properties					627:636	The mechanical properties	612:636	The mechanical properties of GDSF	612:644	The mechanical properties of GDSF were predicted as 375.6 ± 16.58 MPa tensile strength and 126.2 ± 11.93 GPa modulus of elasticity.					
32738327	3	53	theme	G.	281:282	arg1	bark					296:299	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	2	54	theme	damine	208:213	arg1	bark					197:200	the stem bark	188:200	the stem bark of G. damine	188:213	The fiber taken out from the stem bark of G. damine is found to be novel and selected for characterization study.					
32738327	6	55	theme	Crystallinity	563:575	arg1	index					577:581	Crystallinity index	563:581	Crystallinity index of GDSF	563:589	Crystallinity index of GDSF was noted as 30.35%.					
32738327	6	55	theme	Crystallinity	563:575	arg1	%					609:609	30.35%	604:609	30.35%	604:609	Crystallinity index of GDSF was noted as 30.35%.					
32738327	9	56	theme	better	925:930	arg1	strength					940:947	better bonding strength	925:947	better bonding strength between fiber and polymer	925:973	Hence it can be used to prepare composite specimens with better bonding strength between fiber and polymer.					
32738327	3	57	theme	damine	284:289	arg1	bark					296:299	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF)	277:312	The G. damine stem bark fiber (GDSF) has rich in cellulose contents (57.78 ± 3.56%).					
32738327	2	58	theme	G.	205:206	arg1	damine					208:213	G. damine	205:213	G. damine	205:213	The fiber taken out from the stem bark of G. damine is found to be novel and selected for characterization study.					
32738327	8	59	theme	study	790:794	arg1	results					796:802	The morphological and atomic force microscopy study results	744:802	The morphological and atomic force microscopy study results	744:802	The morphological and atomic force microscopy study results revealed that the fiber has rough surface even in its raw form.					
32361092	1	0	theme	bran	135:138	arg1	mechanism					116:124	mechanism	116:124	mechanism	116:124	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	1	0	theme	bran	135:138	arg1	effect					105:110	effect	105:110	effect	105:110	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	6	1	from	modifier	1047:1054	arg1	industry					1068:1075	the food industry	1059:1075	the food industry	1059:1075	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	4	2	theme	α-helix	617:623	arg1	percentage					625:634	α-helix percentage	617:634	α-helix percentage (28.92-63.08%) (p < 0.05)	617:660	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	2	theme	α-helix	617:623	arg1	%					648:648	28.92-63.08%	637:648	28.92-63.08%	637:648	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	2	theme	α-helix	617:623	arg1	p < 0.05					652:659	p < 0.05	652:659	p < 0.05	652:659	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	0	3	theme	bran	85:88	arg1	cellulose					90:98	wheat bran cellulose	79:98	wheat bran cellulose	79:98	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	1	4	dep	bran	135:138	arg1	WBC					151:153	WBC	151:153	WBC	151:153	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	1	4	dep	bran	135:138	arg1	cellulose					140:148	cellulose	140:148	wheat bran cellulose (WBC)	129:154	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	4	5	from	increase	681:688	arg1	%					713:713	16.92-34.37%	702:713	16.92-34.37%	702:713	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	5	from	increase	681:688	arg1	β-turn					731:736	β-turn	731:736	β-turn	731:736	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	5	from	increase	681:688	arg1	p < 0.05					717:724	p < 0.05	717:724	p < 0.05	717:724	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	5	from	increase	681:688	arg1	%					749:749	8.09-13.54%	739:749	8.09-13.54%	739:749	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	5	from	increase	681:688	arg1	β-sheet					693:699	β-sheet	693:699	β-sheet	693:699	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	5	from	increase	681:688	arg1	p > 0.05					753:760	p > 0.05	753:760	p > 0.05	753:760	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	6	theme	concomitant	669:679	arg1	increase					681:688	a concomitant increase	667:688	a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel	667:793	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	7	from	reduction	604:612	arg1	percentage					625:634	α-helix percentage	617:634	α-helix percentage (28.92-63.08%) (p < 0.05)	617:660	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	7	from	reduction	604:612	arg1	%					648:648	28.92-63.08%	637:648	28.92-63.08%	637:648	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	7	from	reduction	604:612	arg1	p < 0.05					652:659	p < 0.05	652:659	p < 0.05	652:659	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	0	8	theme	wheat	79:83	arg1	bran					85:88	wheat bran	79:88	wheat bran cellulose	79:98	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	6	9	theme	novel	1037:1041	arg1	modifier					1047:1054	novel gel modifier	1037:1054	novel gel modifier in the food industry	1037:1075	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	6	10	theme	gel	974:976	arg1	properties					978:987	the gel properties	970:987	the gel properties of SPI	970:994	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	4	11	theme	significant	592:602	arg1	reduction					604:612	a significant reduction	590:612	a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05)	590:660	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	1	12	dep	effect	105:110	arg1	The					101:103	The	101:103	The	101:103	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	6	13	theme	potential	1012:1020	arg1	application					1022:1032	its potential application	1008:1032	its potential application	1008:1032	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	1	14	from	mechanism	116:124	arg1	characteristics					171:185	the gelling characteristics	159:185	the gelling characteristics of soy protein isolate (SPI)	159:214	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	2	15	theme	holding	260:266	arg1	capacity					268:275	the water holding capacity	250:275	the water holding capacity	250:275	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	2	16	theme	gel	319:321	arg1	capacity					268:275	the water holding capacity	250:275	the water holding capacity	250:275	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	2	16	theme	gel	319:321	arg1	strength					282:289	gel strength	278:289	gel strength	278:289	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	2	16	theme	gel	319:321	arg1	viscoelasticity					296:310	viscoelasticity	296:310	viscoelasticity	296:310	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	0	17	theme	Gel	0:2	arg1	properties					4:13	Gel properties	0:13	Gel properties	0:13	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	2	18	theme	water	254:258	arg1	capacity					268:275	the water holding capacity	250:275	the water holding capacity	250:275	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	2	19	theme	SPI	315:317	arg1	gel					319:321	SPI gel	315:321	SPI gel	315:321	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	0	20	theme	formation	19:27	arg1	mechanism					29:37	formation mechanism	19:37	formation mechanism	19:37	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	3	21	dep	WBC	393:395	arg1	w/v					408:410	w/v	408:410	w/v	408:410	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	3	21	dep	WBC	393:395	arg1	%					405:405	0.5-2.0%	398:405	0.5-2.0%	398:405	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	4	22	theme	SPI	787:789	arg1	gel					791:793	the pure SPI gel	778:793	the pure SPI gel	778:793	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	1	23	from	effect	105:110	arg1	characteristics					171:185	the gelling characteristics	159:185	the gelling characteristics of soy protein isolate (SPI)	159:214	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	5	24	theme	hydrogen-bonding	810:825	arg1	interaction					827:837	hydrogen-bonding interaction	810:837	hydrogen-bonding interaction between SPI and WBC and the enhanced thermal stability	810:892	Additionally, hydrogen-bonding interaction between SPI and WBC and the enhanced thermal stability were proposed in the composite gels.					
32361092	3	25	theme	WBC	393:395	arg1	addition					381:388	The addition	377:388	The addition of WBC (0.5-2.0%, w/v)	377:411	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	2	26	theme	WBC	358:360	arg1	concentration					362:374	WBC concentration	358:374	WBC concentration	358:374	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	4	27	theme	pure	782:785	arg1	gel					791:793	the pure SPI gel	778:793	the pure SPI gel	778:793	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	1	28	theme	gelling	163:169	arg1	characteristics					171:185	the gelling characteristics	159:185	the gelling characteristics of soy protein isolate (SPI)	159:214	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	4	29	theme	WBC	570:572	arg1	addition					574:581	WBC addition	570:581	WBC addition	570:581	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	3	30	theme	three-dimensional	502:518	arg1	network					524:530	the regular and homogenous three-dimensional gel network	475:530	the regular and homogenous three-dimensional gel network	475:530	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	4	31	dep	β-sheet	693:699	arg1	percentages					763:773	percentages	763:773	percentages	763:773	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	3	32	theme	regular	479:485	arg1	network					524:530	the regular and homogenous three-dimensional gel network	475:530	the regular and homogenous three-dimensional gel network	475:530	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	4	33	theme	gel	791:793	arg1	β-sheet					693:699	β-sheet	693:699	β-sheet	693:699	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	33	theme	gel	791:793	arg1	%					713:713	16.92-34.37%	702:713	16.92-34.37%	702:713	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	4	33	theme	gel	791:793	arg1	p < 0.05					717:724	p < 0.05	717:724	p < 0.05	717:724	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	2	34	theme	concentration	362:374	arg1	increase					346:353	the increase	342:353	the increase of WBC concentration	342:374	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	0	35	theme	protein	46:52	arg1	gels					62:65	soy protein isolate gels	42:65	soy protein isolate gels	42:65	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	6	36	theme	SPI	992:994	arg1	properties					978:987	the gel properties	970:987	the gel properties of SPI	970:994	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	0	37	theme	soy	42:44	arg1	gels					62:65	soy protein isolate gels	42:65	soy protein isolate gels	42:65	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	3	38	theme	homogenous	491:500	arg1	network					524:530	the regular and homogenous three-dimensional gel network	475:530	the regular and homogenous three-dimensional gel network	475:530	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	2	39	theme	gel	278:280	arg1	strength					282:289	gel strength	278:289	gel strength	278:289	It was found that the water holding capacity, gel strength, and viscoelasticity of SPI gel were improved with the increase of WBC concentration.					
32361092	5	40	theme	composite	915:923	arg1	gels					925:928	the composite gels	911:928	the composite gels	911:928	Additionally, hydrogen-bonding interaction between SPI and WBC and the enhanced thermal stability were proposed in the composite gels.					
32361092	6	41	theme	food	1063:1066	arg1	industry					1068:1075	the food industry	1059:1075	the food industry	1059:1075	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	3	42	theme	gel	520:522	arg1	network					524:530	the regular and homogenous three-dimensional gel network	475:530	the regular and homogenous three-dimensional gel network	475:530	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	1	43	theme	soy	190:192	arg1	isolate					202:208	soy protein isolate	190:208	soy protein isolate (SPI)	190:214	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	1	43	theme	soy	190:192	arg1	SPI					211:213	SPI	211:213	SPI	211:213	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	6	44	theme	gel	1043:1045	arg1	modifier					1047:1054	novel gel modifier	1037:1054	novel gel modifier in the food industry	1037:1075	Overall, WBC is effective in improving the gel properties of SPI, suggesting its potential application as novel gel modifier in the food industry.					
32361092	3	45	theme	moisture	428:435	arg1	phase					437:441	the moisture phase	424:441	the moisture phase	424:441	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	1	46	theme	protein	194:200	arg1	isolate					202:208	soy protein isolate	190:208	soy protein isolate (SPI)	190:214	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	1	46	theme	protein	194:200	arg1	SPI					211:213	SPI	211:213	SPI	211:213	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	0	47	theme	gels	62:65	arg1	properties					4:13	Gel properties	0:13	Gel properties	0:13	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	0	47	theme	gels	62:65	arg1	mechanism					29:37	formation mechanism	19:37	formation mechanism	19:37	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	3	48	theme	network	524:530	arg1	construction					459:470	the construction	455:470	the construction of the regular and homogenous three-dimensional gel network	455:530	The addition of WBC (0.5-2.0%, w/v) stabilized the moisture phase and induced the construction of the regular and homogenous three-dimensional gel network.					
32361092	4	49	theme	Raman	537:541	arg1	spectroscopy					543:554	The Raman spectroscopy	533:554	The Raman spectroscopy	533:554	The Raman spectroscopy revealed that WBC addition caused a significant reduction in α-helix percentage (28.92-63.08%) (p < 0.05) with a concomitant increase in β-sheet (16.92-34.37%) (p < 0.05) and β-turn (8.09-13.54%) (p > 0.05) percentages of the pure SPI gel.					
32361092	5	50	theme	enhanced	867:874	arg1	stability					884:892	the enhanced thermal stability	863:892	the enhanced thermal stability	863:892	Additionally, hydrogen-bonding interaction between SPI and WBC and the enhanced thermal stability were proposed in the composite gels.					
32361092	1	51	theme	isolate	202:208	arg1	characteristics					171:185	the gelling characteristics	159:185	the gelling characteristics of soy protein isolate (SPI)	159:214	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32361092	0	52	theme	isolate	54:60	arg1	gels					62:65	soy protein isolate gels	42:65	soy protein isolate gels	42:65	Gel properties and formation mechanism of soy protein isolate gels improved by wheat bran cellulose.					
32361092	5	53	theme	thermal	876:882	arg1	stability					884:892	the enhanced thermal stability	863:892	the enhanced thermal stability	863:892	Additionally, hydrogen-bonding interaction between SPI and WBC and the enhanced thermal stability were proposed in the composite gels.					
32361092	1	54	theme	wheat	129:133	arg1	bran					135:138	wheat bran cellulose (WBC)	129:154	wheat bran cellulose (WBC)	129:154	The effect and mechanism of wheat bran cellulose (WBC) on the gelling characteristics of soy protein isolate (SPI) were evaluated.					
32693147	13	0	theme	selenide	1808:1815	arg1	microspheres					1824:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	13	1	theme	hybrid	1817:1822	arg1	microspheres					1824:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	5	2	theme	SEM	691:693	arg1	images					695:700	SEM images	691:700	SEM images	691:700	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	8	3	theme	operational	1148:1158	arg1	parameters					1160:1169	operational parameters	1148:1169	operational parameters	1148:1169	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	13	4	theme	industries	1931:1940	arg1	wastewater					1942:1951	the dye and textile industries wastewater	1911:1951	the dye and textile industries wastewater	1911:1951	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	2	5	theme	bismuth	303:309	arg1	nanoparticles					348:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	13	6	theme	fabricated	1773:1782	arg1	microspheres					1824:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	13	7	theme	textile	1923:1929	arg1	industries					1931:1940	textile industries	1923:1940	textile industries	1923:1940	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	2	8	theme	BCSN	328:331	arg1	nanoparticles					348:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	2	9	theme	selenide	318:325	arg1	nanoparticles					348:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	8	10	theme	responsible	1213:1223	arg1	conditions					1202:1211	the conditions	1198:1211	the conditions responsible for maximum dye degradation	1198:1251	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	5	11	theme	nanoparticles	754:766	arg1	size					768:771	an average nanoparticles size	743:771	an average nanoparticles size of 30 nm	743:780	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	7	12	theme	selenium	1027:1034	arg1	presence					994:1001	the presence	990:1001	the presence of bismuth, cobalt, and selenium in the prepared sample	990:1057	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	8	13	theme	maximum	1229:1235	arg1	degradation					1241:1251	maximum dye degradation	1229:1251	maximum dye degradation	1229:1251	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	5	14	theme	smooth	713:718	arg1	morphology					725:734	the smooth face morphology	709:734	the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm	709:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	7	15	theme	prepared	1043:1050	arg1	sample					1052:1057	the prepared sample	1039:1057	the prepared sample	1039:1057	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	3	16	theme	composite	558:566	arg1	recovery					542:549	the easy recovery	533:549	the easy recovery of the composite	533:566	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	1	17	theme	organic	154:160	arg1	pollutants					162:171	hazardous organic pollutants	144:171	hazardous organic pollutants like dyes	144:181	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	10	18	from	%	1463:1463	arg1	irradiation					1527:1537	sunlight irradiation	1518:1537	sunlight irradiation for 100 min	1518:1549	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	5	19	theme	average	789:795	arg1	size					810:813	an average microspheres size	786:813	an average microspheres size of 734 μm	786:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	12	20	dep	five	1750:1753	arg1	to					1747:1748	to	1747:1748	to	1747:1748	Moreover, the prepared sample gave an excellent result after regeneration and reused up to five cycles.					
32693147	0	21	theme	pollutants	91:100	arg1	mitigation					69:78	mitigation	69:78	mitigation of organic pollutants in an aqueous environment	69:126	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	10	22	theme	prepared	1423:1430	arg1	microspheres					1432:1443	prepared microspheres	1423:1443	prepared microspheres	1423:1443	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	13	23	theme	pollutants	1895:1904	arg1	removal					1876:1882	the removal	1872:1882	the removal of organic pollutants from the dye and textile industries wastewater	1872:1951	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	2	24	dep	structure	395:403	arg1	a					381:381	a	381:381	a	381:381	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	9	25	theme	catalyst	1315:1322	arg1	solution					1383:1390	dye solution	1379:1390	dye solution	1379:1390	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	9	25	theme	catalyst	1315:1322	arg1	amount					1324:1329	catalyst amount	1315:1329	catalyst amount	1315:1329	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	8	26	theme	Congo	1060:1064	arg1	CR					1075:1076	CR	1075:1076	CR	1075:1076	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	8	26	theme	Congo	1060:1064	arg1	dye					1070:1072	Congo red dye	1060:1072	Congo red dye (CR)	1060:1077	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	2	27	theme	narrow	409:414	arg1	bandgap					416:422	narrow bandgap	409:422	narrow bandgap	409:422	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	0	28	theme	Regenerable	0:10	arg1	microspheres					52:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres	0:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.	0:127	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	4	29	theme	FTIR	569:572	arg1	studies					574:580	FTIR studies	569:580	FTIR studies	569:580	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	10	30	theme	90-ppm	1472:1477	arg1	solution					1479:1486	the 90-ppm solution	1468:1486	the 90-ppm solution	1468:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	3	31	theme	chitosan	482:489	arg1	microspheres					491:502	chitosan microspheres	482:502	chitosan microspheres	482:502	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	0	32	theme	cobalt	29:34	arg1	microspheres					52:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres	0:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.	0:127	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	7	33	attach	presence	994:1001	arg1	sample					1052:1057	the prepared sample	1039:1057	the prepared sample	1039:1057	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	7	33	attach	presence	994:1001	arg2	selenium					1027:1034	selenium	1027:1034	selenium	1027:1034	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	7	33	attach	presence	994:1001	arg2	cobalt					1015:1020	cobalt	1015:1020	cobalt	1015:1020	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	7	33	attach	presence	994:1001	arg2	bismuth					1006:1012	bismuth	1006:1012	bismuth	1006:1012	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	5	34	contain	having	736:741	arg2	size					810:813	an average microspheres size	786:813	an average microspheres size of 734 μm	786:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	5	34	contain	having	736:741	arg2	size					768:771	an average nanoparticles size	743:771	an average nanoparticles size of 30 nm	743:780	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	5	34	contain	having	736:741	arg1	morphology					725:734	the smooth face morphology	709:734	the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm	709:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	2	35	theme	2.48 eV	428:434	arg1	bandgap					416:422	narrow bandgap	409:422	narrow bandgap	409:422	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	2	35	theme	2.48 eV	428:434	arg1	structure					395:403	crystalline structure	383:403	crystalline structure	383:403	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	0	36	theme	hybrid	45:50	arg1	microspheres					52:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres	0:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.	0:127	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	9	37	theme	dye	1350:1352	arg1	concentration					1354:1366	dye concentration	1350:1366	dye concentration	1350:1366	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	5	38	theme	734 μm	818:823	arg1	size					768:771	an average nanoparticles size	743:771	an average nanoparticles size of 30 nm	743:780	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	5	38	theme	734 μm	818:823	arg1	size					810:813	an average microspheres size	786:813	an average microspheres size of 734 μm	786:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	3	39	from	microspheres	491:502	arg1	nanoparticles					451:463	The prepared nanoparticles	438:463	The prepared nanoparticles	438:463	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	3	39	from	microspheres	491:502	arg1	embedded					470:477	embedded	470:477	embedded	470:477	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	8	40	theme	prepared	1102:1109	arg1	BCSN-CM					1111:1117	the prepared BCSN-CM	1098:1117	the prepared BCSN-CM in sunlight irradiation	1098:1141	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	4	41	theme	chitosan‑bismuth	627:642	arg1	BCSN-CM					681:687	BCSN-CM	681:687	BCSN-CM	681:687	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	4	41	theme	chitosan‑bismuth	627:642	arg1	microspheres					667:678	chitosan‑bismuth cobalt selenide hybrid microspheres	627:678	chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM)	627:688	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	13	42	theme	chitosan-bismuth	1784:1799	arg1	microspheres					1824:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	11	43	theme	CR	1611:1612	arg1	degradation					1596:1606	degradation	1596:1606	degradation of CR	1596:1612	The first-order kinetics was well fitted to degradation of CR having a rate constant of 1.50 × 10-2 min-1.					
32693147	3	44	theme	easy	537:540	arg1	recovery					542:549	the easy recovery	533:549	the easy recovery of the composite	533:566	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	9	45	theme	dye	1379:1381	arg1	solution					1383:1390	dye solution	1379:1390	dye solution	1379:1390	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	4	46	theme	selenide	651:658	arg1	BCSN-CM					681:687	BCSN-CM	681:687	BCSN-CM	681:687	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	4	46	theme	selenide	651:658	arg1	microspheres					667:678	chitosan‑bismuth cobalt selenide hybrid microspheres	627:678	chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM)	627:688	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	10	47	theme	sunlight	1518:1525	arg1	irradiation					1527:1537	sunlight irradiation	1518:1537	sunlight irradiation for 100 min	1518:1549	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	1	48	theme	wastewater	267:276	arg1	treatment					278:286	wastewater treatment	267:286	wastewater treatment	267:286	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	11	49	theme	first-order	1556:1566	arg1	kinetics					1568:1575	The first-order kinetics	1552:1575	The first-order kinetics	1552:1575	The first-order kinetics was well fitted to degradation of CR having a rate constant of 1.50 × 10-2 min-1.					
32693147	2	50	theme	cobalt	311:316	arg1	nanoparticles					348:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	4	51	theme	nanoparticles	609:621	arg1	synthesis					596:604	the synthesis	592:604	the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM)	592:688	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	6	52	theme	crystallite	830:840	arg1	size					842:845	The crystallite size	826:845	The crystallite size of the nanoparticles	826:866	The crystallite size of the nanoparticles was also verified using the XRD technique and found to be 21.3 nm by applying Scherer's equation.					
32693147	6	52	theme	crystallite	830:840	arg1	21.3 nm					926:932	21.3 nm	926:932	21.3 nm	926:932	The crystallite size of the nanoparticles was also verified using the XRD technique and found to be 21.3 nm by applying Scherer's equation.					
32693147	2	53	theme	novel	297:301	arg1	nanoparticles					348:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	1	54	theme	semiconducting	186:199	arg1	materials					201:209	semiconducting materials	186:209	semiconducting materials	186:209	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	8	55	theme	dye	1237:1239	arg1	degradation					1241:1251	maximum dye degradation	1229:1251	maximum dye degradation	1229:1251	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	11	56	theme	1.50 × 10-2 min-1	1640:1656	arg1	constant					1628:1635	a rate constant	1621:1635	a rate constant of 1.50 × 10-2 min-1	1621:1656	The first-order kinetics was well fitted to degradation of CR having a rate constant of 1.50 × 10-2 min-1.					
32693147	10	57	theme	degradation	1397:1407	arg1	%					1463:1463	85%	1461:1463	85% of the 90-ppm solution	1461:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	10	57	theme	degradation	1397:1407	arg1	solution					1479:1486	the 90-ppm solution	1468:1486	the 90-ppm solution	1468:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	10	57	theme	degradation	1397:1407	arg1	efficiency					1409:1418	The degradation efficiency	1393:1418	The degradation efficiency of prepared microspheres	1393:1443	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	4	58	theme	microspheres	667:678	arg1	synthesis					596:604	the synthesis	592:604	the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM)	592:688	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	5	59	theme	average	746:752	arg1	size					768:771	an average nanoparticles size	743:771	an average nanoparticles size of 30 nm	743:780	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	13	60	from	wastewater	1942:1951	arg1	removal					1876:1882	the removal	1872:1882	the removal of organic pollutants from the dye and textile industries wastewater	1872:1951	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	5	61	theme	face	720:723	arg1	morphology					725:734	the smooth face morphology	709:734	the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm	709:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	1	62	theme	hazardous	144:152	arg1	pollutants					162:171	hazardous organic pollutants	144:171	hazardous organic pollutants like dyes	144:181	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	13	63	theme	dye	1915:1917	arg1	wastewater					1942:1951	the dye and textile industries wastewater	1911:1951	the dye and textile industries wastewater	1911:1951	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	0	64	theme	organic	83:89	arg1	pollutants					91:100	organic pollutants	83:100	organic pollutants	83:100	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	7	65	theme	cobalt	1015:1020	arg1	presence					994:1001	the presence	990:1001	the presence of bismuth, cobalt, and selenium in the prepared sample	990:1057	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	1	66	theme	pollutants	162:171	arg1	solutions					253:261	the dynamic solutions	241:261	the dynamic solutions for wastewater treatment	241:286	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	1	66	theme	pollutants	162:171	arg1	Degradation					129:139	Degradation	129:139	Degradation of hazardous organic pollutants like dyes by semiconducting materials	129:209	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	1	66	theme	pollutants	162:171	arg1	one					234:236	one	234:236	one	234:236	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	12	67	theme	excellent	1697:1705	arg1	result					1707:1712	an excellent result	1694:1712	an excellent result	1694:1712	Moreover, the prepared sample gave an excellent result after regeneration and reused up to five cycles.					
32693147	5	68	theme	microspheres	797:808	arg1	size					810:813	an average microspheres size	786:813	an average microspheres size of 734 μm	786:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	9	69	theme	dye	1287:1289	arg1	degradation					1272:1282	degradation	1272:1282	degradation of dye	1272:1289	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	7	70	theme	bismuth	1006:1012	arg1	presence					994:1001	the presence	990:1001	the presence of bismuth, cobalt, and selenium in the prepared sample	990:1057	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	8	71	from	BCSN-CM	1111:1117	arg1	irradiation					1131:1141	sunlight irradiation	1122:1141	sunlight irradiation	1122:1141	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	10	72	theme	microspheres	1432:1443	arg1	%					1463:1463	85%	1461:1463	85% of the 90-ppm solution	1461:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	10	72	theme	microspheres	1432:1443	arg1	solution					1479:1486	the 90-ppm solution	1468:1486	the 90-ppm solution	1468:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	10	72	theme	microspheres	1432:1443	arg1	efficiency					1409:1418	The degradation efficiency	1393:1418	The degradation efficiency of prepared microspheres	1393:1443	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	7	73	from	presence	994:1001	arg1	sample					1052:1057	the prepared sample	1039:1057	the prepared sample	1039:1057	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	13	74	theme	organic	1887:1893	arg1	pollutants					1895:1904	organic pollutants	1887:1904	organic pollutants	1887:1904	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	0	75	theme	aqueous	108:114	arg1	environment					116:126	an aqueous environment	105:126	an aqueous environment	105:126	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	12	76	theme	prepared	1673:1680	arg1	sample					1682:1687	the prepared sample	1669:1687	the prepared sample	1669:1687	Moreover, the prepared sample gave an excellent result after regeneration and reused up to five cycles.					
32693147	0	77	from	mitigation	69:78	arg1	environment					116:126	an aqueous environment	105:126	an aqueous environment	105:126	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	13	78	theme	high	1853:1856	arg1	potential					1858:1866	a high potential	1851:1866	a high potential for the removal of organic pollutants from the dye and textile industries wastewater	1851:1951	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	5	79	theme	30 nm	776:780	arg1	size					768:771	an average nanoparticles size	743:771	an average nanoparticles size of 30 nm	743:780	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	5	79	theme	30 nm	776:780	arg1	size					810:813	an average microspheres size	786:813	an average microspheres size of 734 μm	786:823	SEM images showed the smooth face morphology having an average nanoparticles size of 30 nm and an average microspheres size of 734 μm.					
32693147	8	80	theme	red	1066:1068	arg1	CR					1075:1076	CR	1075:1076	CR	1075:1076	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	8	80	theme	red	1066:1068	arg1	dye					1070:1072	Congo red dye	1060:1072	Congo red dye (CR)	1060:1077	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	0	81	theme	chitosan-bismuth	12:27	arg1	microspheres					52:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres	0:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.	0:127	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	7	82	theme	EDX	966:968	arg1	result					970:975	EDX result	966:975	EDX result	966:975	EDX result demonstrated the presence of bismuth, cobalt, and selenium in the prepared sample.					
32693147	3	83	theme	prepared	442:449	arg1	nanoparticles					451:463	The prepared nanoparticles	438:463	The prepared nanoparticles	438:463	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	3	83	theme	prepared	442:449	arg1	embedded					470:477	embedded	470:477	embedded	470:477	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	0	84	theme	selenide	36:43	arg1	microspheres					52:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres	0:63	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.	0:127	Regenerable chitosan-bismuth cobalt selenide hybrid microspheres for mitigation of organic pollutants in an aqueous environment.					
32693147	9	85	theme	irradiation	1332:1342	arg1	time					1344:1347	irradiation time	1332:1347	irradiation time	1332:1347	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	6	86	theme	XRD	896:898	arg1	technique					900:908	the XRD technique	892:908	the XRD technique	892:908	The crystallite size of the nanoparticles was also verified using the XRD technique and found to be 21.3 nm by applying Scherer's equation.					
32693147	1	87	theme	solutions	253:261	arg1	solutions					253:261	the dynamic solutions	241:261	the dynamic solutions for wastewater treatment	241:286	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	1	87	theme	solutions	253:261	arg1	Degradation					129:139	Degradation	129:139	Degradation of hazardous organic pollutants like dyes by semiconducting materials	129:209	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	1	87	theme	solutions	253:261	arg1	one					234:236	one	234:236	one	234:236	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	3	88	from	embedded	470:477	arg1	microspheres					491:502	chitosan microspheres	482:502	chitosan microspheres	482:502	The prepared nanoparticles were embedded in chitosan microspheres to avoid leaching and ensure the easy recovery of the composite.					
32693147	10	89	theme	optimized	1494:1502	arg1	conditions					1504:1513	optimized conditions	1494:1513	optimized conditions	1494:1513	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	4	90	theme	cobalt	644:649	arg1	BCSN-CM					681:687	BCSN-CM	681:687	BCSN-CM	681:687	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	4	90	theme	cobalt	644:649	arg1	microspheres					667:678	chitosan‑bismuth cobalt selenide hybrid microspheres	627:678	chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM)	627:688	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	10	91	theme	solution	1479:1486	arg1	%					1463:1463	85%	1461:1463	85% of the 90-ppm solution	1461:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	10	91	theme	solution	1479:1486	arg1	solution					1479:1486	the 90-ppm solution	1468:1486	the 90-ppm solution	1468:1486	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	10	91	theme	solution	1479:1486	arg1	efficiency					1409:1418	The degradation efficiency	1393:1418	The degradation efficiency of prepared microspheres	1393:1443	The degradation efficiency of prepared microspheres was found to be 85% of the 90-ppm solution under optimized conditions in sunlight irradiation for 100 min.					
32693147	2	92	theme	tri-composite	334:346	arg1	nanoparticles					348:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles	297:360	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	1	93	theme	dynamic	245:251	arg1	solutions					253:261	the dynamic solutions	241:261	the dynamic solutions for wastewater treatment	241:286	Degradation of hazardous organic pollutants like dyes by semiconducting materials has been considered as one of the dynamic solutions for wastewater treatment.					
32693147	8	94	theme	sunlight	1122:1129	arg1	irradiation					1131:1141	sunlight irradiation	1122:1141	sunlight irradiation	1122:1141	Congo red dye (CR) was degraded using the prepared BCSN-CM in sunlight irradiation, and operational parameters were evaluated to optimize the conditions responsible for maximum dye degradation.					
32693147	6	95	theme	nanoparticles	854:866	arg1	size					842:845	The crystallite size	826:845	The crystallite size of the nanoparticles	826:866	The crystallite size of the nanoparticles was also verified using the XRD technique and found to be 21.3 nm by applying Scherer's equation.					
32693147	6	95	theme	nanoparticles	854:866	arg1	21.3 nm					926:932	21.3 nm	926:932	21.3 nm	926:932	The crystallite size of the nanoparticles was also verified using the XRD technique and found to be 21.3 nm by applying Scherer's equation.					
32693147	4	96	theme	hybrid	660:665	arg1	BCSN-CM					681:687	BCSN-CM	681:687	BCSN-CM	681:687	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	4	96	theme	hybrid	660:665	arg1	microspheres					667:678	chitosan‑bismuth cobalt selenide hybrid microspheres	627:678	chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM)	627:688	FTIR studies confirmed the synthesis of nanoparticles and chitosan‑bismuth cobalt selenide hybrid microspheres (BCSN-CM).					
32693147	13	97	theme	cobalt	1801:1806	arg1	microspheres					1824:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres	1763:1835	The newly fabricated chitosan-bismuth cobalt selenide hybrid microspheres might display a high potential for the removal of organic pollutants from the dye and textile industries wastewater.					
32693147	11	98	theme	rate	1623:1626	arg1	constant					1628:1635	a rate constant	1621:1635	a rate constant of 1.50 × 10-2 min-1	1621:1656	The first-order kinetics was well fitted to degradation of CR having a rate constant of 1.50 × 10-2 min-1.					
32693147	2	99	theme	crystalline	383:393	arg1	structure					395:403	crystalline structure	383:403	crystalline structure	383:403	Herein, novel bismuth cobalt selenide (BCSN) tri-composite nanoparticles were prepared with a crystalline structure and narrow bandgap of (2.48 eV).					
32693147	9	100	theme	solution	1383:1390	arg1	time					1344:1347	irradiation time	1332:1347	irradiation time	1332:1347	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	9	100	theme	solution	1383:1390	arg1	concentration					1354:1366	dye concentration	1350:1366	dye concentration	1350:1366	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	9	100	theme	solution	1383:1390	arg1	pH					1373:1374	pH	1373:1374	pH of dye solution	1373:1390	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	9	100	theme	solution	1383:1390	arg1	solution					1383:1390	dye solution	1379:1390	dye solution	1379:1390	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
32693147	9	100	theme	solution	1383:1390	arg1	amount					1324:1329	catalyst amount	1315:1329	catalyst amount	1315:1329	It was found that degradation of dye depends on factors like catalyst amount, irradiation time, dye concentration, and pH of dye solution.					
33245049	8	0	theme	30	1337:1338	arg1	%					1339:1339	%	1339:1339	%	1339:1339	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	9	1	theme	promising	1515:1523	arg1	material					1534:1541	a promising dressing material	1513:1541	a promising dressing material that accelerates wound healing	1513:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	9	1	theme	promising	1515:1523	arg1	scaffold					1501:1508	the 3D printed CS/BG composite scaffold	1470:1508	the 3D printed CS/BG composite scaffold	1470:1508	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	4	2	theme	printing	702:709	arg1	system					711:716	a home-made cryogenic printing system	680:716	a home-made cryogenic printing system	680:716	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	8	3	theme	other	1428:1432	arg1	groups					1434:1439	other groups	1428:1439	other groups	1428:1439	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	7	4	theme	elongation	1098:1107	arg1	rate					1109:1112	elongation rate	1098:1112	elongation rate	1098:1112	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	4	5	theme	home-made	682:690	arg1	system					711:716	a home-made cryogenic printing system	680:716	a home-made cryogenic printing system	680:716	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	9	6	theme	wound	1560:1564	arg1	healing					1566:1572	wound healing	1560:1572	wound healing	1560:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	2	7	theme	suitable	309:316	arg1	matrix					318:323	a suitable matrix	307:323	a suitable matrix	307:323	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	0	8	theme	wound	110:114	arg1	healing					116:122	wound healing	110:122	wound healing	110:122	Cryogenically printed flexible chitosan/bioglass scaffolds with stable and hierarchical porous structures for wound healing.					
33245049	6	9	theme	absorption	928:937	arg1	capacity					939:946	water absorption capacity	922:946	water absorption capacity	922:946	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	8	10	theme	%	1339:1339	arg1	bioglass					1341:1348	30% bioglass	1337:1348	30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups	1337:1439	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	9	11	theme	3D	1474:1475	arg1	material					1534:1541	a promising dressing material	1513:1541	a promising dressing material that accelerates wound healing	1513:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	9	11	theme	3D	1474:1475	arg1	scaffold					1501:1508	the 3D printed CS/BG composite scaffold	1470:1508	the 3D printed CS/BG composite scaffold	1470:1508	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	6	12	theme	BG	1006:1007	arg1	content					995:1001	the content	991:1001	the content of BG	991:1007	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	7	13	theme	migration	1174:1182	arg1	performance					1184:1194	cell proliferation and migration performance	1151:1194	cell proliferation and migration performance	1151:1194	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	6	14	theme	water	922:926	arg1	capacity					939:946	water absorption capacity	922:946	water absorption capacity	922:946	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	9	15	theme	printed	1477:1483	arg1	material					1534:1541	a promising dressing material	1513:1541	a promising dressing material that accelerates wound healing	1513:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	9	15	theme	printed	1477:1483	arg1	scaffold					1501:1508	the 3D printed CS/BG composite scaffold	1470:1508	the 3D printed CS/BG composite scaffold	1470:1508	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	8	16	theme	better	1358:1363	arg1	closure					1371:1377	better wound closure	1358:1377	better wound closure	1358:1377	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	5	17	theme	scaffold	758:765	arg1	macro-structures					734:749	macro-structures	734:749	macro-structures	734:749	The micro- and macro-structures of the scaffold were highly controllable and reproducible.					
33245049	5	17	theme	scaffold	758:765	arg1	micro-					723:728	micro-	723:728	micro-	723:728	The micro- and macro-structures of the scaffold were highly controllable and reproducible.					
33245049	7	18	dep	in	1127:1128	arg1	vitro					1130:1134	vitro	1130:1134	vitro	1130:1134	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	6	19	theme	printed	814:820	arg1	scaffold					822:829	The printed scaffold	810:829	The printed scaffold	810:829	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	4	20	theme	biological	599:608	arg1	component					610:618	the biological component	595:618	the biological component	595:618	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	4	20	theme	biological	599:608	arg1	chitosan					546:553	chitosan	546:553	chitosan (CS)	546:558	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	7	21	theme	proliferation	1156:1168	arg1	performance					1184:1194	cell proliferation and migration performance	1151:1194	cell proliferation and migration performance	1151:1194	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	4	22	dep	matrix	567:572	arg1	the					563:565	the	563:565	the	563:565	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	5	23	dep	micro-	723:728	arg1	The					719:721	The	719:721	The	719:721	The micro- and macro-structures of the scaffold were highly controllable and reproducible.					
33245049	8	24	theme	composite	1250:1258	arg1	scaffold					1260:1267	the composite scaffold	1246:1267	the composite scaffold	1246:1267	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	3	25	theme	Wound	419:423	arg1	dressings					425:433	Wound dressings	419:433	Wound dressings that allow reproducibility and customizability	419:480	Wound dressings that allow reproducibility and customizability are highly desirable in clinical practice.					
33245049	7	26	theme	tension	1077:1083	arg1	strength					1085:1092	tension strength	1077:1092	tension strength	1077:1092	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	8	27	theme	wound	1365:1369	arg1	closure					1371:1377	better wound closure	1358:1377	better wound closure	1358:1377	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	2	28	theme	wound	404:408	arg1	healing					410:416	wound healing	404:416	wound healing	404:416	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	0	29	theme	chitosan/bioglass	31:47	arg1	scaffolds					49:57	flexible chitosan/bioglass scaffolds	22:57	flexible chitosan/bioglass scaffolds	22:57	Cryogenically printed flexible chitosan/bioglass scaffolds with stable and hierarchical porous structures for wound healing.					
33245049	9	30	theme	composite	1491:1499	arg1	material					1534:1541	a promising dressing material	1513:1541	a promising dressing material that accelerates wound healing	1513:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	9	30	theme	composite	1491:1499	arg1	scaffold					1501:1508	the 3D printed CS/BG composite scaffold	1470:1508	the 3D printed CS/BG composite scaffold	1470:1508	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	7	31	theme	CS	1069:1070	arg1	%					1059:1059	20%	1057:1059	20% that of CS	1057:1070	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	7	31	theme	CS	1069:1070	arg1	CS					1069:1070	CS	1069:1070	CS	1069:1070	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	6	32	theme	interconnected	841:854	arg1	structures					878:887	interconnected and hierarchical pore structures	841:887	interconnected and hierarchical pore structures	841:887	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	3	33	theme	clinical	506:513	arg1	practice					515:522	clinical practice	506:522	clinical practice	506:522	Wound dressings that allow reproducibility and customizability are highly desirable in clinical practice.					
33245049	0	34	theme	flexible	22:29	arg1	scaffolds					49:57	flexible chitosan/bioglass scaffolds	22:57	flexible chitosan/bioglass scaffolds	22:57	Cryogenically printed flexible chitosan/bioglass scaffolds with stable and hierarchical porous structures for wound healing.					
33245049	2	35	contain	possess	299:305	arg2	structure					326:334	structure	326:334	structure	326:334	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	2	35	contain	possess	299:305	arg1	material					283:290	An ideal wound dressing material	259:290	An ideal wound dressing material	259:290	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	2	35	contain	possess	299:305	arg2	matrix					318:323	a suitable matrix	307:323	a suitable matrix	307:323	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	2	35	contain	possess	299:305	arg2	components					350:359	bioactive components	340:359	bioactive components	340:359	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	1	36	with	environment	226:236	arg1	dressing					249:256	wound dressing	243:256	wound dressing	243:256	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	6	37	theme	good	901:904	arg1	flexibility					906:916	good flexibility	901:916	good flexibility	901:916	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	1	38	theme	Wound	125:129	arg1	healing					131:137	Wound healing	125:137	Wound healing	125:137	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	1	38	theme	Wound	125:129	arg1	process					174:180	a dynamic and well-orchestrated process	142:180	a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing	142:256	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	9	39	theme	dressing	1525:1532	arg1	material					1534:1541	a promising dressing material	1513:1541	a promising dressing material that accelerates wound healing	1513:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	9	39	theme	dressing	1525:1532	arg1	scaffold					1501:1508	the 3D printed CS/BG composite scaffold	1470:1508	the 3D printed CS/BG composite scaffold	1470:1508	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	8	40	theme	In	1211:1212	arg1	examinations					1219:1230	In vivo examinations	1211:1230	In vivo examinations	1211:1230	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	8	41	dep	In	1211:1212	arg1	vivo					1214:1217	vivo	1214:1217	vivo	1214:1217	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	8	42	theme	wound	1292:1296	arg1	healing					1298:1304	wound healing	1292:1304	wound healing process	1292:1312	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	8	43	theme	collagen	1403:1410	arg1	deposition					1412:1421	collagen deposition	1403:1421	collagen deposition	1403:1421	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	1	44	theme	optimal	218:224	arg1	environment					226:236	an optimal environment	215:236	an optimal environment with wound dressing	215:256	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	7	45	theme	in	1127:1128	arg1	antibacterial					1136:1148	in vitro antibacterial	1127:1148	in vitro antibacterial	1127:1148	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	3	46	from	desirable	493:501	arg1	practice					515:522	clinical practice	506:522	clinical practice	506:522	Wound dressings that allow reproducibility and customizability are highly desirable in clinical practice.					
33245049	4	47	theme	dressing	642:649	arg1	scaffold					651:658	a spatially designed dressing scaffold	621:658	a spatially designed dressing scaffold	621:658	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	7	48	dep	strength	1085:1092	arg1	the					1073:1075	the	1073:1075	the	1073:1075	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	9	49	theme	CS/BG	1485:1489	arg1	material					1534:1541	a promising dressing material	1513:1541	a promising dressing material that accelerates wound healing	1513:1572	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	9	49	theme	CS/BG	1485:1489	arg1	scaffold					1501:1508	the 3D printed CS/BG composite scaffold	1470:1508	the 3D printed CS/BG composite scaffold	1470:1508	These results indicate that the 3D printed CS/BG composite scaffold is a promising dressing material that accelerates wound healing.					
33245049	0	50	theme	stable	64:69	arg1	structures					95:104	stable and hierarchical porous structures	64:104	stable and hierarchical porous structures for wound healing	64:122	Cryogenically printed flexible chitosan/bioglass scaffolds with stable and hierarchical porous structures for wound healing.					
33245049	8	51	contain	having	1330:1335	arg1	group					1324:1328	the group	1320:1328	the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups	1320:1439	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	8	51	contain	having	1330:1335	arg2	bioglass					1341:1348	30% bioglass	1337:1348	30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups	1337:1439	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	8	52	theme	healing	1298:1304	arg1	process					1306:1312	wound healing process	1292:1312	wound healing process	1292:1312	In vivo examinations revealed that the composite scaffold significantly promoted wound healing process, with the group having 30% bioglass showing better wound closure, neovascularization and collagen deposition than other groups.					
33245049	7	53	theme	BGs	1044:1046	arg1	content					1033:1039	the content	1029:1039	the content of BGs	1029:1046	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	2	54	theme	dressing	274:281	arg1	material					283:290	An ideal wound dressing material	259:290	An ideal wound dressing material	259:290	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	3	55	from	practice	515:522	arg1	desirable					493:501	desirable	493:501	desirable	493:501	Wound dressings that allow reproducibility and customizability are highly desirable in clinical practice.					
33245049	2	56	theme	bioactive	340:348	arg1	components					350:359	bioactive components	340:359	bioactive components	340:359	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	6	57	theme	pore	873:876	arg1	structures					878:887	interconnected and hierarchical pore structures	841:887	interconnected and hierarchical pore structures	841:887	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	2	58	theme	wound	268:272	arg1	dressing					274:281	An ideal wound dressing	259:281	An ideal wound dressing material	259:290	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	1	59	theme	dynamic	144:150	arg1	healing					131:137	Wound healing	125:137	Wound healing	125:137	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	1	59	theme	dynamic	144:150	arg1	process					174:180	a dynamic and well-orchestrated process	142:180	a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing	142:256	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	7	60	dep	%	1059:1059	arg1	that					1061:1064	that	1061:1064	that	1061:1064	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	1	61	theme	wound	243:247	arg1	dressing					249:256	wound dressing	243:256	wound dressing	243:256	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	0	62	theme	porous	88:93	arg1	structures					95:104	stable and hierarchical porous structures	64:104	stable and hierarchical porous structures for wound healing	64:122	Cryogenically printed flexible chitosan/bioglass scaffolds with stable and hierarchical porous structures for wound healing.					
33245049	6	63	theme	hierarchical	860:871	arg1	structures					878:887	interconnected and hierarchical pore structures	841:887	interconnected and hierarchical pore structures	841:887	The printed scaffold exhibited interconnected and hierarchical pore structures, as well as good flexibility and water absorption capacity, and these properties were not affected by the content of BG.					
33245049	2	64	theme	ideal	262:266	arg1	dressing					274:281	An ideal wound dressing	259:281	An ideal wound dressing material	259:290	An ideal wound dressing material should possess a suitable matrix, structure and bioactive components, functioning synergistically to accelerate wound healing.					
33245049	4	65	theme	designed	633:640	arg1	scaffold					651:658	a spatially designed dressing scaffold	621:658	a spatially designed dressing scaffold	621:658	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	4	66	theme	cryogenic	692:700	arg1	system					711:716	a home-made cryogenic printing system	680:716	a home-made cryogenic printing system	680:716	In this study, using chitosan (CS) as the matrix and bioglass (BG) as the biological component, a spatially designed dressing scaffold was fabricated from a home-made cryogenic printing system.					
33245049	0	67	theme	hierarchical	75:86	arg1	structures					95:104	stable and hierarchical porous structures	64:104	stable and hierarchical porous structures for wound healing	64:122	Cryogenically printed flexible chitosan/bioglass scaffolds with stable and hierarchical porous structures for wound healing.					
33245049	7	68	theme	cell	1151:1154	arg1	proliferation					1156:1168	cell proliferation	1151:1168	cell proliferation	1151:1168	Nevertheless, when the content of BGs exceeded 20% that of CS, the tension strength and elongation rate reduced, but in vitro antibacterial, cell proliferation and migration performance were enhanced.					
33245049	1	69	theme	well-orchestrated	156:172	arg1	healing					131:137	Wound healing	125:137	Wound healing	125:137	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
33245049	1	69	theme	well-orchestrated	156:172	arg1	process					174:180	a dynamic and well-orchestrated process	142:180	a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing	142:256	Wound healing is a dynamic and well-orchestrated process that can be promoted by creating an optimal environment with wound dressing.					
32068056	7	0	theme	adsorption	1353:1362	arg1	554.4 mg/g					1389:1398	554.4 mg/g	1389:1398	554.4 mg/g	1389:1398	The maximum adsorption capacity was found to be 554.4 mg/g.					
32068056	7	0	theme	adsorption	1353:1362	arg1	capacity					1364:1371	The maximum adsorption capacity	1341:1371	The maximum adsorption capacity	1341:1371	The maximum adsorption capacity was found to be 554.4 mg/g.					
32068056	2	1	theme	magnetic	353:360	arg1	ZnO/nanocellulose					362:378	the thiourea-modified magnetic ZnO/nanocellulose composite	331:388	the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II)	331:463	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	2	1	theme	magnetic	353:360	arg1	TZFNC					391:395	TZFNC	391:395	TZFNC	391:395	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	4	2	theme	removal	929:935	arg1	efficiency					908:917	the efficiency	904:917	the efficiency of Pb(II) removal	904:935	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	2	theme	removal	929:935	arg1	%					945:945	99.99%	940:945	99.99%	940:945	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	3	3	theme	ions	750:753	arg1	parameters					729:738	operational parameters	717:738	operational parameters (pH, lead ions concentration, adsorbent dosage and contact time)	717:803	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	3	theme	ions	750:753	arg1	concentration					755:767	lead ions concentration	745:767	lead ions concentration	745:767	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	0	4	from	removal	11:17	arg1	solutions					47:55	aqueous solutions	39:55	aqueous solutions	39:55	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	8	5	theme	good	1405:1408	arg1	performance					1421:1431	The good adsorption performance	1401:1431	The good adsorption performance	1401:1431	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	2	6	theme	thiourea-modified	335:351	arg1	ZnO/nanocellulose					362:378	the thiourea-modified magnetic ZnO/nanocellulose composite	331:388	the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II)	331:463	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	2	6	theme	thiourea-modified	335:351	arg1	TZFNC					391:395	TZFNC	391:395	TZFNC	391:395	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	3	7	theme	contact	791:797	arg1	time					799:802	contact time	791:802	contact time	791:802	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	7	theme	contact	791:797	arg1	parameters					729:738	operational parameters	717:738	operational parameters (pH, lead ions concentration, adsorbent dosage and contact time)	717:803	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	0	8	theme	magnetic	84:91	arg1	composite					111:119	thiourea-functionalized magnetic ZnO/nanocellulose composite	60:119	thiourea-functionalized magnetic ZnO/nanocellulose composite	60:119	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	3	9	theme	surface	639:645	arg1	methodology					647:657	Response surface methodology	630:657	Response surface methodology (RSM)	630:663	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	9	theme	surface	639:645	arg1	RSM					660:662	RSM	660:662	RSM	660:662	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	10	from	influence	704:712	arg1	efficiency					820:829	the removal efficiency	808:829	the removal efficiency of lead ions with TZFNC	808:853	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	0	11	theme	thiourea-functionalized	60:82	arg1	composite					111:119	thiourea-functionalized magnetic ZnO/nanocellulose composite	60:119	thiourea-functionalized magnetic ZnO/nanocellulose composite	60:119	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	3	12	theme	Response	630:637	arg1	methodology					647:657	Response surface methodology	630:657	Response surface methodology (RSM)	630:663	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	12	theme	Response	630:637	arg1	RSM					660:662	RSM	660:662	RSM	660:662	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	1	13	theme	low	261:263	arg1	cost					265:268	their low cost	255:268	their low cost	255:268	Removal of pollutants by bio-superadsorbent is currently in the limelight due to their low cost and biocompatibility with the environment.					
32068056	2	14	theme	physicochemical	500:514	arg1	properties					516:525	its physicochemical properties	496:525	its physicochemical properties	496:525	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	5	15	theme	Langmuir	1196:1203	arg1	isotherm					1205:1212	the Langmuir isotherm and Pseudo-Second-Order model	1192:1242	isotherm	1205:1212	The equilibrium, and kinetic studies suggested that the adsorption process followed the Langmuir isotherm and Pseudo-Second-Order model.					
32068056	8	16	theme	contaminated	1557:1568	arg1	wastewater					1570:1579	Pb(II) contaminated wastewater	1550:1579	Pb(II) contaminated wastewater	1550:1579	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	0	17	theme	ZnO/nanocellulose	93:109	arg1	composite					111:119	thiourea-functionalized magnetic ZnO/nanocellulose composite	60:119	thiourea-functionalized magnetic ZnO/nanocellulose composite	60:119	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	1	18	with	biocompatibility	274:289	arg1	environment					300:310	the environment	296:310	the environment	296:310	Removal of pollutants by bio-superadsorbent is currently in the limelight due to their low cost and biocompatibility with the environment.					
32068056	4	19	theme	Pb	922:923	arg1	removal					929:935	Pb(II) removal	922:935	Pb(II) removal	922:935	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	5	20	theme	Pseudo-Second-Order	1218:1236	arg1	model					1238:1242	the Langmuir isotherm and Pseudo-Second-Order model	1192:1242	model	1238:1242	The equilibrium, and kinetic studies suggested that the adsorption process followed the Langmuir isotherm and Pseudo-Second-Order model.					
32068056	6	21	theme	Thermodynamic	1245:1257	arg1	data					1259:1262	Thermodynamic data	1245:1262	Thermodynamic data	1245:1262	Thermodynamic data showed that the sorption process was feasible, spontaneous, and endothermic.					
32068056	5	22	theme	kinetic	1129:1135	arg1	studies					1137:1143	kinetic studies	1129:1143	kinetic studies	1129:1143	The equilibrium, and kinetic studies suggested that the adsorption process followed the Langmuir isotherm and Pseudo-Second-Order model.					
32068056	7	23	theme	maximum	1345:1351	arg1	554.4 mg/g					1389:1398	554.4 mg/g	1389:1398	554.4 mg/g	1389:1398	The maximum adsorption capacity was found to be 554.4 mg/g.					
32068056	7	23	theme	maximum	1345:1351	arg1	capacity					1364:1371	The maximum adsorption capacity	1341:1371	The maximum adsorption capacity	1341:1371	The maximum adsorption capacity was found to be 554.4 mg/g.					
32068056	8	24	dep	performance	1421:1431	arg1	ability					1477:1483	ability	1477:1483	ability	1477:1483	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	3	25	theme	removal	812:818	arg1	efficiency					820:829	the removal efficiency	808:829	the removal efficiency of lead ions with TZFNC	808:853	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	0	26	theme	Adsorptive	0:9	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).	0:172	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	8	27	theme	easy	1452:1455	arg1	separation					1466:1475	easy magnetic separation	1452:1475	easy magnetic separation	1452:1475	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	1	28	with	cost	265:268	arg1	environment					300:310	the environment	296:310	the environment	296:310	Removal of pollutants by bio-superadsorbent is currently in the limelight due to their low cost and biocompatibility with the environment.					
32068056	4	29	theme	experimental	979:990	arg1	time = 14.5 min					1090:1104	contact time = 14.5 min	1082:1104	contact time = 14.5 min	1082:1104	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	29	theme	experimental	979:990	arg1	amount					1015:1020	the amount	1011:1020	the amount of adsorbent = 40 mg	1011:1041	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	29	theme	experimental	979:990	arg1	concentration = 60 mg L-1					1051:1075	Pb(II) concentration = 60 mg L-1	1044:1075	Pb(II) concentration = 60 mg L-1	1044:1075	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	29	theme	experimental	979:990	arg1	factors					992:998	experimental factors	979:998	experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	979:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	29	theme	experimental	979:990	arg1	adsorbent = 40 mg					1025:1041	adsorbent = 40 mg	1025:1041	adsorbent = 40 mg	1025:1041	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	29	theme	experimental	979:990	arg1	pH = 6.5					1001:1008	pH = 6.5	1001:1008	pH = 6.5	1001:1008	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	3	30	with	ions	839:842	arg1	TZFNC					849:853	TZFNC	849:853	TZFNC	849:853	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	0	31	theme	Pb	22:23	arg1	ions					29:32	Pb(II) ions	22:32	Pb(II) ions from aqueous solutions	22:55	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	4	32	theme	contact	1082:1088	arg1	factors					992:998	experimental factors	979:998	experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	979:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	32	theme	contact	1082:1088	arg1	time = 14.5 min					1090:1104	contact time = 14.5 min	1082:1104	contact time = 14.5 min	1082:1104	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	3	33	theme	lead	745:748	arg1	ions					750:753	lead ions	745:753	lead ions concentration	745:767	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	1	34	theme	pollutants	185:194	arg1	Removal					174:180	Removal	174:180	Removal of pollutants by bio-superadsorbent	174:216	Removal of pollutants by bio-superadsorbent is currently in the limelight due to their low cost and biocompatibility with the environment.					
32068056	2	35	theme	separation	432:441	arg1	efficiency					443:452	separation efficiency	432:452	separation efficiency	432:452	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	8	36	theme	adsorption	1410:1419	arg1	performance					1421:1431	The good adsorption performance	1401:1431	The good adsorption performance	1401:1431	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	4	37	theme	factors	992:998	arg1	conditions					965:974	the optimum conditions	953:974	the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	953:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	3	38	theme	lead	834:837	arg1	ions					839:842	lead ions	834:842	lead ions with TZFNC	834:853	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	0	39	theme	II	25:26	arg1	ions					29:32	Pb(II) ions	22:32	Pb(II) ions from aqueous solutions	22:55	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	8	40	theme	magnetic	1457:1464	arg1	separation					1466:1475	easy magnetic separation	1452:1475	easy magnetic separation	1452:1475	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	0	41	theme	response	138:145	arg1	methodology					155:165	response surface methodology	138:165	response surface methodology (RSM)	138:171	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	0	41	theme	response	138:145	arg1	RSM					168:170	RSM	168:170	RSM	168:170	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	0	42	from	solutions	47:55	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).	0:172	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	0	42	from	solutions	47:55	arg1	ions					29:32	Pb(II) ions	22:32	Pb(II) ions from aqueous solutions	22:55	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	3	43	theme	ions	839:842	arg1	efficiency					820:829	the removal efficiency	808:829	the removal efficiency of lead ions with TZFNC	808:853	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	2	44	theme	adsorption	408:417	arg1	capacity					419:426	high adsorption capacity	403:426	high adsorption capacity	403:426	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	8	45	theme	great	1514:1518	arg1	potential					1520:1528	great potential	1514:1528	great potential for purification of Pb(II) contaminated wastewater	1514:1579	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	2	46	theme	high	403:406	arg1	capacity					419:426	high adsorption capacity	403:426	high adsorption capacity	403:426	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	0	47	theme	ions	29:32	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).	0:172	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	4	48	dep	factors	992:998	arg1	time = 14.5 min					1090:1104	contact time = 14.5 min	1082:1104	contact time = 14.5 min	1082:1104	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	48	dep	factors	992:998	arg1	amount					1015:1020	the amount	1011:1020	the amount of adsorbent = 40 mg	1011:1041	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	48	dep	factors	992:998	arg1	concentration = 60 mg L-1					1051:1075	Pb(II) concentration = 60 mg L-1	1044:1075	Pb(II) concentration = 60 mg L-1	1044:1075	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	48	dep	factors	992:998	arg1	factors					992:998	experimental factors	979:998	experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	979:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	48	dep	factors	992:998	arg1	adsorbent = 40 mg					1025:1041	adsorbent = 40 mg	1025:1041	adsorbent = 40 mg	1025:1041	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	48	dep	factors	992:998	arg1	pH = 6.5					1001:1008	pH = 6.5	1001:1008	pH = 6.5	1001:1008	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	3	49	dep	parameters	729:738	arg1	dosage					780:785	adsorbent dosage	770:785	adsorbent dosage	770:785	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	49	dep	parameters	729:738	arg1	time					799:802	contact time	791:802	contact time	791:802	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	49	dep	parameters	729:738	arg1	pH					741:742	pH	741:742	pH	741:742	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	49	dep	parameters	729:738	arg1	parameters					729:738	operational parameters	717:738	operational parameters (pH, lead ions concentration, adsorbent dosage and contact time)	717:803	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	49	dep	parameters	729:738	arg1	concentration					755:767	lead ions concentration	745:767	lead ions concentration	745:767	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	5	50	theme	adsorption	1164:1173	arg1	process					1175:1181	the adsorption process	1160:1181	the adsorption process	1160:1181	The equilibrium, and kinetic studies suggested that the adsorption process followed the Langmuir isotherm and Pseudo-Second-Order model.					
32068056	0	51	theme	surface	147:153	arg1	methodology					155:165	response surface methodology	138:165	response surface methodology (RSM)	138:171	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	0	51	theme	surface	147:153	arg1	RSM					168:170	RSM	168:170	RSM	168:170	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	4	52	theme	optimum	957:963	arg1	conditions					965:974	the optimum conditions	953:974	the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	953:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	8	53	contain	has	1510:1512	arg1	TZFNC					1504:1508	the TZFNC	1500:1508	the TZFNC	1500:1508	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	8	53	contain	has	1510:1512	arg2	potential					1520:1528	great potential	1514:1528	great potential for purification of Pb(II) contaminated wastewater	1514:1579	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	8	54	theme	Pb	1550:1551	arg1	wastewater					1570:1579	Pb(II) contaminated wastewater	1550:1579	Pb(II) contaminated wastewater	1550:1579	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	4	55	theme	adsorbent = 40 mg	1025:1041	arg1	time = 14.5 min					1090:1104	contact time = 14.5 min	1082:1104	contact time = 14.5 min	1082:1104	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	55	theme	adsorbent = 40 mg	1025:1041	arg1	amount					1015:1020	the amount	1011:1020	the amount of adsorbent = 40 mg	1011:1041	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	55	theme	adsorbent = 40 mg	1025:1041	arg1	concentration = 60 mg L-1					1051:1075	Pb(II) concentration = 60 mg L-1	1044:1075	Pb(II) concentration = 60 mg L-1	1044:1075	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	55	theme	adsorbent = 40 mg	1025:1041	arg1	factors					992:998	experimental factors	979:998	experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	979:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	55	theme	adsorbent = 40 mg	1025:1041	arg1	adsorbent = 40 mg					1025:1041	adsorbent = 40 mg	1025:1041	adsorbent = 40 mg	1025:1041	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	55	theme	adsorbent = 40 mg	1025:1041	arg1	pH = 6.5					1001:1008	pH = 6.5	1001:1008	pH = 6.5	1001:1008	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	0	56	theme	aqueous	39:45	arg1	solutions					47:55	aqueous solutions	39:55	aqueous solutions	39:55	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	2	57	with	ZnO/nanocellulose	362:378	arg1	capacity					419:426	high adsorption capacity	403:426	high adsorption capacity	403:426	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	2	57	with	ZnO/nanocellulose	362:378	arg1	efficiency					443:452	separation efficiency	432:452	separation efficiency	432:452	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	0	58	dep	removal	11:17	arg1	Optimization					122:133	Optimization	122:133	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).	0:172	Adsorptive removal of Pb(II) ions from aqueous solutions by thiourea-functionalized magnetic ZnO/nanocellulose composite: Optimization by response surface methodology (RSM).					
32068056	2	59	theme	composite	380:388	arg1	ZnO/nanocellulose					362:378	the thiourea-modified magnetic ZnO/nanocellulose composite	331:388	the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II)	331:463	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	2	59	theme	composite	380:388	arg1	TZFNC					391:395	TZFNC	391:395	TZFNC	391:395	In this research, the thiourea-modified magnetic ZnO/nanocellulose composite (TZFNC) with high adsorption capacity and separation efficiency for Pb(II) was prepared successfully, and its physicochemical properties were characterized via XRD, SEM, TEM, AFM, BET, FTIR, XPS, EDAX, Zeta-potential and VSM, respectively.					
32068056	3	60	theme	adsorbent	770:778	arg1	dosage					780:785	adsorbent dosage	770:785	adsorbent dosage	770:785	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	60	theme	adsorbent	770:778	arg1	parameters					729:738	operational parameters	717:738	operational parameters (pH, lead ions concentration, adsorbent dosage and contact time)	717:803	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	61	theme	operational	717:727	arg1	dosage					780:785	adsorbent dosage	770:785	adsorbent dosage	770:785	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	61	theme	operational	717:727	arg1	time					799:802	contact time	791:802	contact time	791:802	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	61	theme	operational	717:727	arg1	pH					741:742	pH	741:742	pH	741:742	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	61	theme	operational	717:727	arg1	parameters					729:738	operational parameters	717:738	operational parameters (pH, lead ions concentration, adsorbent dosage and contact time)	717:803	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	61	theme	operational	717:727	arg1	concentration					755:767	lead ions concentration	745:767	lead ions concentration	745:767	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	6	62	theme	sorption	1280:1287	arg1	feasible					1301:1308	feasible	1301:1308	feasible	1301:1308	Thermodynamic data showed that the sorption process was feasible, spontaneous, and endothermic.					
32068056	6	62	theme	sorption	1280:1287	arg1	process					1289:1295	the sorption process	1276:1295	the sorption process	1276:1295	Thermodynamic data showed that the sorption process was feasible, spontaneous, and endothermic.					
32068056	4	63	theme	Pb	1044:1045	arg1	factors					992:998	experimental factors	979:998	experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min)	979:1105	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	4	63	theme	Pb	1044:1045	arg1	concentration = 60 mg L-1					1051:1075	Pb(II) concentration = 60 mg L-1	1044:1075	Pb(II) concentration = 60 mg L-1	1044:1075	The results indicated that the efficiency of Pb(II) removal is 99.99% under the optimum conditions of experimental factors (pH = 6.5, the amount of adsorbent = 40 mg, Pb(II) concentration = 60 mg L-1, and contact time = 14.5 min).					
32068056	8	64	theme	wastewater	1570:1579	arg1	purification					1534:1545	purification	1534:1545	purification of Pb(II) contaminated wastewater	1534:1579	The good adsorption performance, recyclability and easy magnetic separation ability made sure that the TZFNC has great potential for purification of Pb(II) contaminated wastewater.					
32068056	3	65	theme	parameters	729:738	arg1	adsorbent					866:874	adsorbent	866:874	adsorbent	866:874	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
32068056	3	65	theme	parameters	729:738	arg1	influence					704:712	the influence	700:712	the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC	700:853	Response surface methodology (RSM) has been applied in order to study the influence of operational parameters (pH, lead ions concentration, adsorbent dosage and contact time) on the removal efficiency of lead ions with TZFNC as a novel adsorbent.					
33436441	8	0	theme	wall	1499:1502	arg1	biogenesis					1504:1513	cell wall biogenesis	1494:1513	cell wall biogenesis	1494:1513	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	10	1	theme	antifungal	1844:1853	arg1	therapies					1855:1863	safe and effective antifungal therapies	1825:1863	safe and effective antifungal therapies	1825:1863	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	7	2	theme	wall	1346:1349	arg1	reliant					1376:1382	reliant	1376:1382	reliant	1376:1382	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	7	2	theme	wall	1346:1349	arg1	composition					1351:1361	a cell wall composition	1339:1361	a cell wall composition that is less reliant on β-1,3-glucan	1339:1398	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	7	3	theme	increased	1302:1310	arg1	content					1319:1325	increased chitin content	1302:1325	increased chitin content	1302:1325	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	11	4	theme	antifungal	1909:1918	arg1	development					1925:1935	antifungal drug development	1909:1935	antifungal drug development	1909:1935	Yet, antifungal drug development has stalled in the pharmaceutical industry.					
33436441	5	5	theme	mRNA	857:860	arg1	abundance					835:843	the abundance	831:843	the abundance of the FKS1 mRNA	831:860	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	6	6	theme	β-1,6-glucan	1148:1159	arg1	gene					1170:1173	a β-1,6-glucan synthase gene	1146:1173	chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1)	1055:1180	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	6	theme	β-1,6-glucan	1148:1159	arg1	SKN1					1176:1179	SKN1	1176:1179	SKN1	1176:1179	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	1	7	theme	antifungal	224:233	arg1	caspofungin					240:250	the echinocandin antifungal drug caspofungin	207:250	the echinocandin antifungal drug caspofungin	207:250	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	1	8	theme	drugs	405:409	arg1	class					385:389	the newest class	374:389	the newest class of antifungal drugs	374:409	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	1	8	theme	drugs	405:409	arg1	they					365:368	they	365:368	they	365:368	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	3	9	theme	PUF4	593:596	arg1	overexpression					575:588	overexpression	575:588	overexpression of PUF4	575:596	In contrast, overexpression of PUF4 resulted in caspofungin sensitivity.					
33436441	4	10	from	elements	677:684	arg1	region					716:721	its 5' untranslated region	696:721	its 5' untranslated region	696:721	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	11	11	theme	pharmaceutical	1956:1969	arg1	industry					1971:1978	the pharmaceutical industry	1952:1978	the pharmaceutical industry	1952:1978	Yet, antifungal drug development has stalled in the pharmaceutical industry.					
33436441	5	12	dep	region	760:765	arg1	destabilized					793:804	destabilized	793:804	was destabilized in the puf4Δ mutant	789:824	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	5	12	dep	region	760:765	arg1	reduced					866:872	reduced	866:872	reduced	866:872	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	7	13	theme	wall	1251:1254	arg1	components					1256:1265	cell wall components	1246:1265	cell wall components	1246:1265	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	1	14	theme	fungal	137:142	arg1	pathogen					144:151	The human fungal pathogen Cryptococcus neoformans	127:175	The human fungal pathogen Cryptococcus neoformans	127:175	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	1	14	theme	fungal	137:142	arg1	resistant					194:202	resistant	194:202	resistant	194:202	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	10	15	theme	significant	1739:1749	arg1	lack					1817:1820	the lack	1813:1820	the lack of safe and effective antifungal therapies	1813:1863	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	10	15	theme	significant	1739:1749	arg1	contributor					1751:1761	A significant contributor	1737:1761	A significant contributor to the high morbidity and mortality statistics	1737:1808	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	1	16	theme	Cryptococcus	153:164	arg1	neoformans					166:175	Cryptococcus neoformans	153:175	The human fungal pathogen Cryptococcus neoformans	127:175	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	7	17	theme	dyes	1229:1232	arg1	use					1210:1212	The use	1206:1212	The use of fluorescent dyes to quantify cell wall components	1206:1265	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	4	18	theme	untranslated	703:714	arg1	region					716:721	its 5' untranslated region	696:721	its 5' untranslated region	696:721	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	0	19	theme	Resistance	88:97	arg1	Regulation					35:44	Post-transcriptional Regulation	14:44	Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans	14:124	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	1	20	dep	pathogen	144:151	arg1	neoformans					166:175	Cryptococcus neoformans	153:175	The human fungal pathogen Cryptococcus neoformans	127:175	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	0	21	theme	Cryptococcus	102:113	arg1	neoformans					115:124	Cryptococcus neoformans	102:124	Cryptococcus neoformans	102:124	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	1	22	theme	newest	378:383	arg1	class					385:389	the newest class	374:389	the newest class of antifungal drugs	374:409	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	1	22	theme	newest	378:383	arg1	they					365:368	they	365:368	they	365:368	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	5	23	theme	FKS1	945:948	arg1	stability					955:963	FKS1 mRNA stability	945:963	FKS1 mRNA stability	945:963	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	2	24	theme	caspofungin	538:548	arg1	resistance					550:559	exacerbated caspofungin resistance	526:559	exacerbated caspofungin resistance	526:559	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	6	25	dep	FKS1	981:984	arg1	addition					969:976	addition	969:976	addition	969:976	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	9	26	theme	deaths	1729:1734	arg1	%					1711:1711	15%	1709:1711	15% of AIDS-related deaths	1709:1734	It is also responsible for 15% of AIDS-related deaths.					
33436441	9	26	theme	deaths	1729:1734	arg1	deaths					1729:1734	AIDS-related deaths	1716:1734	AIDS-related deaths	1716:1734	It is also responsible for 15% of AIDS-related deaths.					
33436441	5	27	theme	stability	955:963	arg1	regulator					932:940	a positive regulator	921:940	a positive regulator of FKS1 mRNA stability	921:963	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	5	27	theme	stability	955:963	arg1	Puf4					913:916	Puf4	913:916	Puf4	913:916	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	7	28	theme	fluorescent	1217:1227	arg1	dyes					1229:1232	fluorescent dyes	1217:1232	fluorescent dyes	1217:1232	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	2	29	theme	pumilio/FBF	465:475	arg1	Puf4					511:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	0	30	theme	Caspofungin	76:86	arg1	Resistance					88:97	Caspofungin Resistance	76:97	Caspofungin Resistance	76:97	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	6	31	theme	biosynthesis	1025:1036	arg1	gene					1170:1173	a β-1,6-glucan synthase gene	1146:1173	chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1)	1055:1180	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	31	theme	biosynthesis	1025:1036	arg1	genes					1038:1042	additional cell wall biosynthesis genes	1004:1042	additional cell wall biosynthesis genes	1004:1042	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	31	theme	biosynthesis	1025:1036	arg1	deacetylases					1099:1110	deacetylases	1099:1110	deacetylases	1099:1110	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	31	theme	biosynthesis	1025:1036	arg1	synthases					1062:1070	chitin synthases	1055:1070	chitin synthases (CHS3, CHS4, and CHS6)	1055:1093	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	2	32	theme	mutant	445:450	arg1	Analysis					412:419	Analysis	412:419	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4,	412:515	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	0	33	theme	Post-transcriptional	14:33	arg1	Regulation					35:44	Post-transcriptional Regulation	14:44	Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans	14:124	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	7	34	contain	had	1298:1300	arg1	mutant					1291:1296	the puf4Δ mutant	1281:1296	the puf4Δ mutant	1281:1296	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	7	34	contain	had	1298:1300	arg2	content					1319:1325	increased chitin content	1302:1325	increased chitin content	1302:1325	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	6	35	theme	cell	1015:1018	arg1	gene					1170:1173	a β-1,6-glucan synthase gene	1146:1173	chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1)	1055:1180	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	35	theme	cell	1015:1018	arg1	genes					1038:1042	additional cell wall biosynthesis genes	1004:1042	additional cell wall biosynthesis genes	1004:1042	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	35	theme	cell	1015:1018	arg1	deacetylases					1099:1110	deacetylases	1099:1110	deacetylases	1099:1110	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	35	theme	cell	1015:1018	arg1	synthases					1062:1070	chitin synthases	1055:1070	chitin synthases (CHS3, CHS4, and CHS6)	1055:1093	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	2	36	theme	family	497:502	arg1	Puf4					511:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	6	37	dep	synthases	1062:1070	arg1	CDA3					1129:1132	CDA3	1129:1132	CDA3	1129:1132	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	37	dep	synthases	1062:1070	arg1	CHS4					1079:1082	CHS4	1079:1082	CHS4	1079:1082	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	37	dep	synthases	1062:1070	arg1	CDA1					1113:1116	CDA1	1113:1116	CDA1	1113:1116	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	37	dep	synthases	1062:1070	arg1	synthases					1062:1070	chitin synthases	1055:1070	chitin synthases (CHS3, CHS4, and CHS6)	1055:1093	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	37	dep	synthases	1062:1070	arg1	CDA2					1119:1122	CDA2	1119:1122	CDA2	1119:1122	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	37	dep	synthases	1062:1070	arg1	CHS6					1089:1092	CHS6	1089:1092	CHS6	1089:1092	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	37	dep	synthases	1062:1070	arg1	CHS3					1073:1076	CHS3	1073:1076	CHS3	1073:1076	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	2	38	theme	C.	426:427	arg1	mutant					445:450	a C. neoformanspuf4Δ mutant	424:450	a C. neoformanspuf4Δ mutant	424:450	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	2	39	theme	binding	481:487	arg1	Puf4					511:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	0	40	theme	Wall	54:57	arg1	Biosynthesis					59:70	Cell Wall Biosynthesis	49:70	Cell Wall Biosynthesis	49:70	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	10	41	theme	mortality	1789:1797	arg1	statistics					1799:1808	the high morbidity and mortality statistics	1766:1808	the high morbidity and mortality statistics	1766:1808	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	4	42	theme	FKS1	639:642	arg1	mRNA					644:647	The FKS1 mRNA	635:647	The FKS1 mRNA	635:647	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	10	43	theme	morbidity	1775:1783	arg1	statistics					1799:1808	the high morbidity and mortality statistics	1766:1808	the high morbidity and mortality statistics	1766:1808	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	1	44	theme	β-1,3-glucan	271:282	arg1	synthase					284:291	the β-1,3-glucan synthase	267:291	the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs	267:409	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	6	45	theme	chitin	1055:1060	arg1	CDA3					1129:1132	CDA3	1129:1132	CDA3	1129:1132	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	45	theme	chitin	1055:1060	arg1	CHS4					1079:1082	CHS4	1079:1082	CHS4	1079:1082	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	45	theme	chitin	1055:1060	arg1	CDA1					1113:1116	CDA1	1113:1116	CDA1	1113:1116	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	45	theme	chitin	1055:1060	arg1	synthases					1062:1070	chitin synthases	1055:1070	chitin synthases (CHS3, CHS4, and CHS6)	1055:1093	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	45	theme	chitin	1055:1060	arg1	CDA2					1119:1122	CDA2	1119:1122	CDA2	1119:1122	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	45	theme	chitin	1055:1060	arg1	CHS6					1089:1092	CHS6	1089:1092	CHS6	1089:1092	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	45	theme	chitin	1055:1060	arg1	CHS3					1073:1076	CHS3	1073:1076	CHS3	1073:1076	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	8	46	theme	cell	1494:1497	arg1	biogenesis					1504:1513	cell wall biogenesis	1494:1513	cell wall biogenesis	1494:1513	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	11	47	theme	drug	1920:1923	arg1	development					1925:1935	antifungal drug development	1909:1935	antifungal drug development	1909:1935	Yet, antifungal drug development has stalled in the pharmaceutical industry.					
33436441	1	48	theme	FKS1	304:307	arg1	Echinocandins					309:321	FKS1 Echinocandins	304:321	FKS1 Echinocandins	304:321	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	7	49	theme	cell	1341:1344	arg1	reliant					1376:1382	reliant	1376:1382	reliant	1376:1382	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	7	49	theme	cell	1341:1344	arg1	composition					1351:1361	a cell wall composition	1339:1361	a cell wall composition that is less reliant on β-1,3-glucan	1339:1398	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	10	50	theme	effective	1834:1842	arg1	therapies					1855:1863	safe and effective antifungal therapies	1825:1863	safe and effective antifungal therapies	1825:1863	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	8	51	theme	environmental	1600:1612	arg1	fungus					1614:1619	an environmental fungus	1597:1619	an environmental fungus that causes pulmonary and central nervous system infections	1597:1679	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	5	52	theme	FKS1	852:855	arg1	mRNA					857:860	the FKS1 mRNA	848:860	the FKS1 mRNA	848:860	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	7	53	theme	chitin	1312:1317	arg1	content					1319:1325	increased chitin content	1302:1325	increased chitin content	1302:1325	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	10	54	theme	safe	1825:1828	arg1	therapies					1855:1863	safe and effective antifungal therapies	1825:1863	safe and effective antifungal therapies	1825:1863	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	6	55	theme	synthase	1161:1168	arg1	gene					1170:1173	a β-1,6-glucan synthase gene	1146:1173	chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1)	1055:1180	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	55	theme	synthase	1161:1168	arg1	SKN1					1176:1179	SKN1	1176:1179	SKN1	1176:1179	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	1	56	theme	echinocandin	211:222	arg1	caspofungin					240:250	the echinocandin antifungal drug caspofungin	207:250	the echinocandin antifungal drug caspofungin	207:250	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	8	57	theme	pulmonary	1633:1641	arg1	infections					1670:1679	pulmonary and central nervous system infections	1633:1679	pulmonary and central nervous system infections	1633:1679	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	1	58	theme	drug	235:238	arg1	caspofungin					240:250	the echinocandin antifungal drug caspofungin	207:250	the echinocandin antifungal drug caspofungin	207:250	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	10	59	from	lack	1817:1820	arg1	settings					1894:1901	resource-poor settings	1880:1901	resource-poor settings	1880:1901	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	7	60	theme	puf4Δ	1285:1289	arg1	mutant					1291:1296	the puf4Δ mutant	1281:1296	the puf4Δ mutant	1281:1296	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	4	61	theme	Puf4-binding	664:675	arg1	PBEs					687:690	PBEs	687:690	PBEs	687:690	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	4	61	theme	Puf4-binding	664:675	arg1	elements					677:684	three Puf4-binding elements	658:684	three Puf4-binding elements (PBEs) in its 5' untranslated region	658:721	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	10	62	theme	therapies	1855:1863	arg1	lack					1817:1820	the lack	1813:1820	the lack of safe and effective antifungal therapies	1813:1863	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	10	62	theme	therapies	1855:1863	arg1	contributor					1751:1761	A significant contributor	1737:1761	A significant contributor to the high morbidity and mortality statistics	1737:1808	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	8	63	theme	central	1647:1653	arg1	system					1663:1668	system	1663:1668	system	1663:1668	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	3	64	theme	caspofungin	610:620	arg1	sensitivity					622:632	caspofungin sensitivity	610:632	caspofungin sensitivity	610:632	In contrast, overexpression of PUF4 resulted in caspofungin sensitivity.					
33436441	8	65	theme	system	1663:1668	arg1	infections					1670:1679	pulmonary and central nervous system infections	1633:1679	pulmonary and central nervous system infections	1633:1679	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	1	66	theme	human	131:135	arg1	pathogen					144:151	The human fungal pathogen Cryptococcus neoformans	127:175	The human fungal pathogen Cryptococcus neoformans	127:175	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	1	66	theme	human	131:135	arg1	resistant					194:202	resistant	194:202	resistant	194:202	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	10	67	theme	resource-poor	1880:1892	arg1	settings					1894:1901	resource-poor settings	1880:1901	resource-poor settings	1880:1901	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	4	68	contain	contains	649:656	arg1	mRNA					644:647	The FKS1 mRNA	635:647	The FKS1 mRNA	635:647	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	4	68	contain	contains	649:656	arg2	PBEs					687:690	PBEs	687:690	PBEs	687:690	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	4	68	contain	contains	649:656	arg2	elements					677:684	three Puf4-binding elements	658:684	three Puf4-binding elements (PBEs) in its 5' untranslated region	658:721	The FKS1 mRNA contains three Puf4-binding elements (PBEs) in its 5' untranslated region.					
33436441	7	69	theme	cell	1246:1249	arg1	components					1256:1265	cell wall components	1246:1265	cell wall components	1246:1265	The use of fluorescent dyes to quantify cell wall components revealed that the puf4Δ mutant had increased chitin content, suggesting a cell wall composition that is less reliant on β-1,3-glucan.					
33436441	5	70	theme	FKS1	770:773	arg1	region					760:765	this region	755:765	this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability	755:963	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	8	71	dep	suggest	1423:1429	arg1	fungus					1614:1619	an environmental fungus	1597:1619	an environmental fungus that causes pulmonary and central nervous system infections	1597:1679	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	0	72	from	Regulation	35:44	arg1	neoformans					115:124	Cryptococcus neoformans	102:124	Cryptococcus neoformans	102:124	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	5	73	theme	FKS1	779:782	arg1	mRNA					784:787	The FKS1 mRNA	775:787	The FKS1 mRNA	775:787	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	5	74	theme	wild	886:889	arg1	type					891:894	wild type	886:894	wild type	886:894	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	2	75	theme	member	504:509	arg1	Puf4					511:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	8	76	theme	nervous	1655:1661	arg1	system					1663:1668	system	1663:1668	system	1663:1668	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	5	77	theme	mRNA	950:953	arg1	stability					955:963	FKS1 mRNA stability	945:963	FKS1 mRNA stability	945:963	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	2	78	theme	exacerbated	526:536	arg1	resistance					550:559	exacerbated caspofungin resistance	526:559	exacerbated caspofungin resistance	526:559	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	5	79	theme	puf4Δ	813:817	arg1	mutant					819:824	the puf4Δ mutant	809:824	the puf4Δ mutant	809:824	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	1	80	theme	antifungal	394:403	arg1	drugs					405:409	antifungal drugs	394:409	antifungal drugs	394:409	The human fungal pathogen Cryptococcus neoformans is intrinsically resistant to the echinocandin antifungal drug caspofungin, which targets the β-1,3-glucan synthase encoded by FKS1 Echinocandins have been on the market for 20 years, yet they are the newest class of antifungal drugs.					
33436441	12	81	theme	important	2164:2172	arg1	pathogen					2174:2181	this important pathogen	2159:2181	this important pathogen	2159:2181	Therefore, it is essential to understand the mechanism by which C. neoformans is resistant to caspofungin to design adjunctive therapies to potentiate the drug's activity toward this important pathogen.					
33436441	6	82	theme	genes	1038:1042	arg1	abundance					991:999	the abundance	987:999	the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1),	987:1181	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	5	83	theme	positive	923:930	arg1	regulator					932:940	a positive regulator	921:940	a positive regulator of FKS1 mRNA stability	921:963	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	5	83	theme	positive	923:930	arg1	Puf4					913:916	Puf4	913:916	Puf4	913:916	Puf4 binds with specificity to this region of FKS1 The FKS1 mRNA was destabilized in the puf4Δ mutant, and the abundance of the FKS1 mRNA was reduced compared to wild type, suggesting that Puf4 is a positive regulator of FKS1 mRNA stability.					
33436441	6	84	theme	wall	1020:1023	arg1	gene					1170:1173	a β-1,6-glucan synthase gene	1146:1173	chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1)	1055:1180	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	84	theme	wall	1020:1023	arg1	genes					1038:1042	additional cell wall biosynthesis genes	1004:1042	additional cell wall biosynthesis genes	1004:1042	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	84	theme	wall	1020:1023	arg1	deacetylases					1099:1110	deacetylases	1099:1110	deacetylases	1099:1110	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	84	theme	wall	1020:1023	arg1	synthases					1062:1070	chitin synthases	1055:1070	chitin synthases (CHS3, CHS4, and CHS6)	1055:1093	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	9	85	theme	AIDS-related	1716:1727	arg1	deaths					1729:1734	AIDS-related deaths	1716:1734	AIDS-related deaths	1716:1734	It is also responsible for 15% of AIDS-related deaths.					
33436441	10	86	theme	high	1770:1773	arg1	morbidity					1775:1783	high morbidity	1770:1783	high morbidity	1770:1783	A significant contributor to the high morbidity and mortality statistics is the lack of safe and effective antifungal therapies, especially in resource-poor settings.					
33436441	2	87	theme	neoformanspuf4Δ	429:443	arg1	mutant					445:450	a C. neoformanspuf4Δ mutant	424:450	a C. neoformanspuf4Δ mutant	424:450	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	0	88	theme	Cell	49:52	arg1	Biosynthesis					59:70	Cell Wall Biosynthesis	49:70	Cell Wall Biosynthesis	49:70	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	6	89	theme	additional	1004:1013	arg1	gene					1170:1173	a β-1,6-glucan synthase gene	1146:1173	chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1)	1055:1180	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	89	theme	additional	1004:1013	arg1	genes					1038:1042	additional cell wall biosynthesis genes	1004:1042	additional cell wall biosynthesis genes	1004:1042	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	89	theme	additional	1004:1013	arg1	deacetylases					1099:1110	deacetylases	1099:1110	deacetylases	1099:1110	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	6	89	theme	additional	1004:1013	arg1	synthases					1062:1070	chitin synthases	1055:1070	chitin synthases (CHS3, CHS4, and CHS6)	1055:1093	In addition to FKS1, the abundance of additional cell wall biosynthesis genes, including chitin synthases (CHS3, CHS4, and CHS6) and deacetylases (CDA1, CDA2, and CDA3) as well as a β-1,6-glucan synthase gene (SKN1), was regulated by Puf4.					
33436441	2	90	theme	protein	489:495	arg1	Puf4					511:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	8	91	theme	caspofungin	1452:1462	arg1	resistance					1464:1473	caspofungin resistance	1452:1473	caspofungin resistance	1452:1473	Overall, our findings suggest a mechanism by which caspofungin resistance, and more broadly, cell wall biogenesis, is regulated post-transcriptionally by Puf4.IMPORTANCECryptococcus neoformans is an environmental fungus that causes pulmonary and central nervous system infections.					
33436441	0	92	theme	Biosynthesis	59:70	arg1	Regulation					35:44	Post-transcriptional Regulation	14:44	Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans	14:124	Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.					
33436441	2	93	theme	RNA	477:479	arg1	Puf4					511:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	the pumilio/FBF RNA binding protein family member Puf4	461:514	Analysis of a C. neoformanspuf4Δ mutant, lacking the pumilio/FBF RNA binding protein family member Puf4, revealed exacerbated caspofungin resistance.					
33436441	12	94	theme	adjunctive	2097:2106	arg1	therapies					2108:2116	adjunctive therapies	2097:2116	adjunctive therapies	2097:2116	Therefore, it is essential to understand the mechanism by which C. neoformans is resistant to caspofungin to design adjunctive therapies to potentiate the drug's activity toward this important pathogen.					
33858076	10	0	theme	well	1562:1565	arg1	method					1577:1582	the well diffusion method	1558:1582	the well diffusion method	1558:1582	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	8	1	theme	oxygen	1016:1021	arg1	vacancies					1023:1031	oxygen vacancies	1016:1031	oxygen vacancies	1016:1031	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	14	2	theme	human	2149:2153	arg1	conditions					2162:2171	human health conditions	2149:2171	human health conditions	2149:2171	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	10	3	theme	diffusion	1567:1575	arg1	method					1577:1582	the well diffusion method	1558:1582	the well diffusion method	1558:1582	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	2	4	theme	nanoparticles	353:365	arg1	synthesis					273:281	the synthesis	269:281	the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs)	269:371	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	8	5	theme	active	1080:1085	arg1	radicals					1087:1094	the active radicals	1076:1094	the active radicals	1076:1094	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	8	5	theme	active	1080:1085	arg1	responsible					1118:1128	responsible	1118:1128	responsible	1118:1128	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	11	6	theme	cell	1635:1638	arg1	MCF-7					1646:1650	breast cancer cell line (MCF-7)	1621:1651	breast cancer cell line (MCF-7)	1621:1651	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	2	7	theme	oxide	296:300	arg1	synthesis					273:281	the synthesis	269:281	the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs)	269:371	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	9	8	theme	cell	1291:1294	arg1	line					1296:1299	the MCF-7 cell line	1281:1299	the MCF-7 cell line	1281:1299	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	12	9	theme	higher	1776:1781	arg1	cytotoxicity					1793:1804	cytotoxicity	1793:1804	cytotoxicity	1793:1804	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	12	9	theme	higher	1776:1781	arg1	amount					1783:1788	a higher amount	1774:1788	a higher amount of cytotoxicity for both the bacterial and cancer cells	1774:1844	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	11	10	theme	breast	1621:1626	arg1	MCF-7					1646:1650	breast cancer cell line (MCF-7)	1621:1651	breast cancer cell line (MCF-7)	1621:1651	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	13	11	theme	fibroblast	1896:1905	arg1	cells					1912:1916	fibroblast L929 cells	1896:1916	fibroblast L929 cells	1896:1916	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	2	12	theme	magnesium	330:338	arg1	oxide					340:344	chitosan-modified magnesium oxide	312:344	chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs)	312:371	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	2	12	theme	magnesium	330:338	arg1	CMgO					347:350	CMgO	347:350	CMgO	347:350	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	4	13	theme	XRD	575:577	arg1	studies					579:585	XRD studies	575:585	XRD studies	575:585	The MgO and CMgO NPs exhibit face-centered cubic structures, as confirmed by XRD studies.					
33858076	13	14	theme	cells	1912:1916	arg1	studies					1885:1891	Toxicity studies	1876:1891	Toxicity studies of fibroblast L929 cells	1876:1916	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	9	15	theme	different	1309:1317	arg1	types					1319:1323	six different types	1305:1323	six different types of gram-negative bacteria	1305:1349	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	12	16	theme	CMgO	1758:1761	arg1	NPs					1763:1765	CMgO NPs	1758:1765	CMgO NPs	1758:1765	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	5	17	theme	FESEM	609:613	arg1	images					623:628	the FESEM and TEM images	605:628	the FESEM and TEM images	605:628	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	5	18	dep	MgO	646:648	arg1	NPs					659:661	NPs	659:661	NPs	659:661	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	5	18	dep	MgO	646:648	arg1	the					642:644	the	642:644	the	642:644	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	14	19	theme	healthcare	2094:2103	arg1	applications					2116:2127	healthcare industrial applications	2094:2127	healthcare industrial applications	2094:2127	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	5	20	theme	TEM	619:621	arg1	images					623:628	the FESEM and TEM images	605:628	the FESEM and TEM images	605:628	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	9	21	theme	bacteria	1342:1349	arg1	types					1319:1323	six different types	1305:1323	six different types of gram-negative bacteria	1305:1349	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	9	21	theme	bacteria	1342:1349	arg1	line					1296:1299	the MCF-7 cell line	1281:1299	the MCF-7 cell line	1281:1299	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	1	22	theme	eco-friendly	127:138	arg1	substances					165:174	eco-friendly multifunctional biocidal substances	127:174	eco-friendly multifunctional biocidal substances with low cost and high efficiency	127:208	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	13	23	theme	CMgO	1936:1939	arg1	NPs					1941:1943	the CMgO NPs	1932:1943	the CMgO NPs	1932:1943	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	13	23	theme	CMgO	1936:1939	arg1	harmful					1955:1961	harmful	1955:1961	harmful	1955:1961	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	7	24	theme	stretching	861:870	arg1	frequency					872:880	the Mg-O stretching frequency	852:880	the Mg-O stretching frequency of MgO and CMgO NPs	852:900	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	1	25	theme	substances	165:174	arg1	development					112:122	the development	108:122	the development	108:122	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	1	26	theme	low	181:183	arg1	cost					185:188	low cost	181:188	low cost	181:188	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	12	27	theme	MgO	1867:1869	arg1	NPs					1871:1873	the MgO NPs	1863:1873	the MgO NPs	1863:1873	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	4	28	dep	MgO	502:504	arg1	NPs					515:517	NPs	515:517	NPs	515:517	The MgO and CMgO NPs exhibit face-centered cubic structures, as confirmed by XRD studies.					
33858076	4	28	dep	MgO	502:504	arg1	The					498:500	The	498:500	The	498:500	The MgO and CMgO NPs exhibit face-centered cubic structures, as confirmed by XRD studies.					
33858076	1	29	with	substances	165:174	arg1	cost					185:188	low cost	181:188	low cost	181:188	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	1	29	with	substances	165:174	arg1	efficiency					199:208	high efficiency	194:208	high efficiency	194:208	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	1	30	theme	multifunctional	140:154	arg1	substances					165:174	eco-friendly multifunctional biocidal substances	127:174	eco-friendly multifunctional biocidal substances with low cost and high efficiency	127:208	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	9	31	dep	viability	1222:1230	arg1	anticancer					1251:1260	anticancer	1251:1260	anticancer	1251:1260	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	9	31	dep	viability	1222:1230	arg1	antibacterial					1233:1245	antibacterial	1233:1245	antibacterial	1233:1245	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	14	32	theme	biopolymer	2041:2050	arg1	NPs					2074:2076	biopolymer chitosan-modified MgO NPs	2041:2076	biopolymer chitosan-modified MgO NPs	2041:2076	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	11	33	theme	higher	1706:1711	arg1	properties					1724:1733	higher anticancer properties	1706:1733	higher anticancer properties than MgO NPs	1706:1746	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	9	34	theme	toxicity	1165:1172	arg1	effects					1174:1180	The toxicity effects	1161:1180	The toxicity effects	1161:1180	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	3	35	dep	Plumbago	444:451	arg1	L					463:463	L	463:463	Plumbago zeylanica L	444:463	In this process, leaves extract of Plumbago zeylanica L was, used as a nucleating agent.					
33858076	3	35	dep	Plumbago	444:451	arg1	zeylanica					453:461	Plumbago zeylanica L	444:463	Plumbago zeylanica L	444:463	In this process, leaves extract of Plumbago zeylanica L was, used as a nucleating agent.					
33858076	7	36	located	observed	907:914	arg1	431					919:921	431	919:921	431	919:921	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	7	36	located	observed	907:914	arg1	435 cm-1					927:934	435 cm-1	927:934	435 cm-1	927:934	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	7	36	located	observed	907:914	arg2	frequency					872:880	the Mg-O stretching frequency	852:880	the Mg-O stretching frequency of MgO and CMgO NPs	852:900	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	10	37	theme	Proteus	1493:1499	arg1	vulgaris					1501:1508	Proteus vulgaris	1493:1508	Proteus vulgaris	1493:1508	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	37	theme	Proteus	1493:1499	arg1	activities					1370:1379	The antibacterial activities	1352:1379	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae	1352:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	0	38	theme	Biocidal	0:7	arg1	oxide					28:32	Biocidal chitosan-magnesium oxide	0:32	Biocidal chitosan-magnesium oxide	0:32	Biocidal chitosan-magnesium oxide nanoparticles via a green precipitation process.					
33858076	2	39	theme	precipitation	386:398	arg1	process					400:406	a green precipitation process	378:406	a green precipitation process	378:406	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	11	40	theme	MgO	1740:1742	arg1	NPs					1744:1746	MgO NPs	1740:1746	MgO NPs	1740:1746	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	9	41	theme	nanoparticles	1189:1201	arg1	effects					1174:1180	The toxicity effects	1161:1180	The toxicity effects	1161:1180	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	14	42	used	used	2085:2088	arg2	NPs					2074:2076	biopolymer chitosan-modified MgO NPs	2041:2076	biopolymer chitosan-modified MgO NPs	2041:2076	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	7	43	dep	MgO	885:887	arg1	NPs					898:900	NPs	898:900	NPs	898:900	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	5	44	theme	average	687:693	arg1	size					704:707	an average particle size	684:707	an average particle size of ~40±2 and ~37±2 nm	684:729	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	12	45	theme	cytotoxicity	1793:1804	arg1	cytotoxicity					1793:1804	cytotoxicity	1793:1804	cytotoxicity	1793:1804	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	12	45	theme	cytotoxicity	1793:1804	arg1	amount					1783:1788	a higher amount	1774:1788	a higher amount of cytotoxicity for both the bacterial and cancer cells	1774:1844	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	2	46	theme	chitosan-modified	312:328	arg1	oxide					340:344	chitosan-modified magnesium oxide	312:344	chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs)	312:371	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	2	46	theme	chitosan-modified	312:328	arg1	CMgO					347:350	CMgO	347:350	CMgO	347:350	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	6	47	used	used	763:766	arg2	spectra					750:756	EDX spectra	746:756	EDX spectra	746:756	EDX spectra were used to identify the elemental compositions of the nanoparticles.					
33858076	9	48	theme	cell	1217:1220	arg1	viability					1222:1230	cell viability	1217:1230	cell viability (antibacterial and anticancer)	1217:1261	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	4	49	theme	face-centered	527:539	arg1	structures					547:556	face-centered cubic structures	527:556	face-centered cubic structures	527:556	The MgO and CMgO NPs exhibit face-centered cubic structures, as confirmed by XRD studies.					
33858076	1	50	theme	focus	236:240	arg1	center					226:231	the center	222:231	the center of focus	222:240	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	5	51	theme	particle	695:702	arg1	size					704:707	an average particle size	684:707	an average particle size of ~40±2 and ~37±2 nm	684:729	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	6	52	theme	nanoparticles	814:826	arg1	compositions					794:805	the elemental compositions	780:805	the elemental compositions of the nanoparticles	780:826	EDX spectra were used to identify the elemental compositions of the nanoparticles.					
33858076	8	53	dep	MgO	989:991	arg1	NPs					1002:1004	NPs	1002:1004	NPs	1002:1004	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	2	54	theme	oxide	340:344	arg1	NPs					368:370	NPs	368:370	NPs	368:370	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	2	54	theme	oxide	340:344	arg1	nanoparticles					353:365	chitosan-modified magnesium oxide (CMgO) nanoparticles	312:365	chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs)	312:371	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	6	55	theme	EDX	746:748	arg1	spectra					750:756	EDX spectra	746:756	EDX spectra	746:756	EDX spectra were used to identify the elemental compositions of the nanoparticles.					
33858076	14	56	theme	health	2155:2160	arg1	conditions					2162:2171	human health conditions	2149:2171	human health conditions	2149:2171	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	3	57	theme	nucleating	480:489	arg1	agent					491:495	a nucleating agent	478:495	a nucleating agent	478:495	In this process, leaves extract of Plumbago zeylanica L was, used as a nucleating agent.					
33858076	6	58	theme	elemental	784:792	arg1	compositions					794:805	the elemental compositions	780:805	the elemental compositions of the nanoparticles	780:826	EDX spectra were used to identify the elemental compositions of the nanoparticles.					
33858076	10	59	theme	Klebsiella	1406:1415	arg1	pneumoniae					1417:1426	Klebsiella pneumoniae	1406:1426	Klebsiella pneumoniae	1406:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	60	dep	coli	1441:1444	arg1	bacteria					1530:1537	bacteria	1530:1537	bacteria	1530:1537	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	9	61	theme	MCF-7	1285:1289	arg1	line					1296:1299	the MCF-7 cell line	1281:1299	the MCF-7 cell line	1281:1299	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	0	62	theme	green	54:58	arg1	process					74:80	a green precipitation process	52:80	a green precipitation process	52:80	Biocidal chitosan-magnesium oxide nanoparticles via a green precipitation process.					
33858076	3	63	used	used	470:473	arg2	extract					433:439	extract	433:439	extract of Plumbago zeylanica L	433:463	In this process, leaves extract of Plumbago zeylanica L was, used as a nucleating agent.					
33858076	10	64	theme	Shigella	1447:1454	arg1	dysenteriae					1456:1466	Shigella dysenteriae	1447:1466	Shigella dysenteriae	1447:1466	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	64	theme	Shigella	1447:1454	arg1	activities					1370:1379	The antibacterial activities	1352:1379	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae	1352:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	1	65	theme	high	194:197	arg1	efficiency					199:208	high efficiency	194:208	high efficiency	194:208	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	9	66	from	effects	1174:1180	arg1	viability					1222:1230	cell viability	1217:1230	cell viability (antibacterial and anticancer)	1217:1261	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	11	67	theme	line	1640:1643	arg1	MCF-7					1646:1650	breast cancer cell line (MCF-7)	1621:1651	breast cancer cell line (MCF-7)	1621:1651	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	2	68	theme	magnesium	286:294	arg1	MgO					303:305	MgO	303:305	MgO	303:305	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	2	68	theme	magnesium	286:294	arg1	oxide					296:300	magnesium oxide	286:300	magnesium oxide (MgO)	286:306	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	5	69	theme	nm	728:729	arg1	size					704:707	an average particle size	684:707	an average particle size of ~40±2 and ~37±2 nm	684:729	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	11	70	theme	cancer	1628:1633	arg1	MCF-7					1646:1650	breast cancer cell line (MCF-7)	1621:1651	breast cancer cell line (MCF-7)	1621:1651	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	11	71	dep	MgO	1589:1591	arg1	The					1585:1587	The	1585:1587	The	1585:1587	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	11	71	dep	MgO	1589:1591	arg1	NPs					1602:1604	NPs	1602:1604	NPs	1602:1604	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	14	72	theme	industrial	2105:2114	arg1	applications					2116:2127	healthcare industrial applications	2094:2127	healthcare industrial applications	2094:2127	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	13	73	theme	Toxicity	1876:1883	arg1	studies					1885:1891	Toxicity studies	1876:1891	Toxicity studies of fibroblast L929 cells	1876:1916	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	7	74	theme	CMgO	893:896	arg1	frequency					872:880	the Mg-O stretching frequency	852:880	the Mg-O stretching frequency of MgO and CMgO NPs	852:900	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	9	75	theme	gram-negative	1328:1340	arg1	bacteria					1342:1349	gram-negative bacteria	1328:1349	gram-negative bacteria	1328:1349	The toxicity effects of the nanoparticles developed, on cell viability (antibacterial and anticancer), were measured on the MCF-7 cell line and six different types of gram-negative bacteria.					
33858076	8	76	theme	biocidal	1140:1147	arg1	activities					1149:1158	their biocidal activities	1134:1158	their biocidal activities	1134:1158	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	14	77	theme	MgO	2070:2072	arg1	NPs					2074:2076	biopolymer chitosan-modified MgO NPs	2041:2076	biopolymer chitosan-modified MgO NPs	2041:2076	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	7	78	theme	MgO	885:887	arg1	frequency					872:880	the Mg-O stretching frequency	852:880	the Mg-O stretching frequency of MgO and CMgO NPs	852:900	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	1	79	theme	biocidal	156:163	arg1	substances					165:174	eco-friendly multifunctional biocidal substances	127:174	eco-friendly multifunctional biocidal substances with low cost and high efficiency	127:208	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	5	80	with	spherical	668:676	arg1	size					704:707	an average particle size	684:707	an average particle size of ~40±2 and ~37±2 nm	684:729	Morphologically, the FESEM and TEM images showed that the MgO and CMgO NPs were spherical, with an average particle size of ~40±2 and ~37±2 nm, respectively.					
33858076	12	81	theme	cancer	1833:1838	arg1	cells					1840:1844	cancer cells	1833:1844	cancer cells	1833:1844	Overall, CMgO NPs showed a higher amount of cytotoxicity for both the bacterial and cancer cells when compared to the MgO NPs.					
33858076	7	82	theme	Mg-O	856:859	arg1	frequency					872:880	the Mg-O stretching frequency	852:880	the Mg-O stretching frequency of MgO and CMgO NPs	852:900	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	10	83	from	activities	1370:1379	arg1	pneumoniae					1417:1426	Klebsiella pneumoniae	1406:1426	Klebsiella pneumoniae	1406:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	14	84	theme	chitosan-modified	2052:2068	arg1	NPs					2074:2076	biopolymer chitosan-modified MgO NPs	2041:2076	biopolymer chitosan-modified MgO NPs	2041:2076	These results suggest that biopolymer chitosan-modified MgO NPs can be used for healthcare industrial applications in order to improve human health conditions.					
33858076	11	85	contain	possess	1698:1704	arg1	NPs					1694:1696	CMgO NPs	1689:1696	CMgO NPs	1689:1696	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	11	85	contain	possess	1698:1704	arg2	properties					1724:1733	higher anticancer properties	1706:1733	higher anticancer properties than MgO NPs	1706:1746	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	11	86	theme	anticancer	1713:1722	arg1	properties					1724:1733	higher anticancer properties	1706:1733	higher anticancer properties than MgO NPs	1706:1746	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	0	87	theme	chitosan-magnesium	9:26	arg1	oxide					28:32	Biocidal chitosan-magnesium oxide	0:32	Biocidal chitosan-magnesium oxide	0:32	Biocidal chitosan-magnesium oxide nanoparticles via a green precipitation process.					
33858076	7	88	theme	FTIR	838:841	arg1	spectra					843:849	FTIR spectra	838:849	FTIR spectra	838:849	By using FTIR spectra, the Mg-O stretching frequency of MgO and CMgO NPs were observed at 431 and 435 cm-1, respectively.					
33858076	1	89	theme	present	90:96	arg1	scenario					98:105	the present scenario	86:105	the present scenario	86:105	In the present scenario, the development of eco-friendly multifunctional biocidal substances with low cost and high efficiency, has become the center of focus.					
33858076	13	90	theme	L929	1907:1910	arg1	cells					1912:1916	fibroblast L929 cells	1896:1916	fibroblast L929 cells	1896:1916	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	8	91	theme	photoluminescence	955:971	arg1	spectra					978:984	The photoluminescence (PL) spectra	951:984	The photoluminescence (PL) spectra	951:984	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	2	92	theme	green	380:384	arg1	process					400:406	a green precipitation process	378:406	a green precipitation process	378:406	This study is, focused on the synthesis of magnesium oxide (MgO) and chitosan-modified magnesium oxide (CMgO) nanoparticles (NPs), via a green precipitation process.					
33858076	13	93	theme	MgO	2005:2007	arg1	NPs					2009:2011	the MgO NPs	2001:2011	the MgO NPs	2001:2011	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	10	94	theme	Vibrio	1514:1519	arg1	cholerae					1521:1528	Vibrio cholerae	1514:1528	Vibrio cholerae	1514:1528	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	94	theme	Vibrio	1514:1519	arg1	activities					1370:1379	The antibacterial activities	1352:1379	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae	1352:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	0	95	theme	precipitation	60:72	arg1	process					74:80	a green precipitation process	52:80	a green precipitation process	52:80	Biocidal chitosan-magnesium oxide nanoparticles via a green precipitation process.					
33858076	13	96	theme	healthy	1970:1976	arg1	cells					1978:1982	the healthy cells	1966:1982	the healthy cells	1966:1982	Toxicity studies of fibroblast L929 cells revealed that the CMgO NPs were less harmful to the healthy cells when compared to the MgO NPs.					
33858076	10	97	from	nanoparticles	1388:1400	arg1	pneumoniae					1417:1426	Klebsiella pneumoniae	1406:1426	Klebsiella pneumoniae	1406:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	98	theme	antibacterial	1356:1368	arg1	activities					1370:1379	The antibacterial activities	1352:1379	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae	1352:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	98	theme	antibacterial	1356:1368	arg1	vulgaris					1501:1508	Proteus vulgaris	1493:1508	Proteus vulgaris	1493:1508	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	98	theme	antibacterial	1356:1368	arg1	dysenteriae					1456:1466	Shigella dysenteriae	1447:1466	Shigella dysenteriae	1447:1466	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	98	theme	antibacterial	1356:1368	arg1	aeruginosa					1481:1490	Pseudomonas aeruginosa	1469:1490	Pseudomonas aeruginosa	1469:1490	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	98	theme	antibacterial	1356:1368	arg1	cholerae					1521:1528	Vibrio cholerae	1514:1528	Vibrio cholerae	1514:1528	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	98	theme	antibacterial	1356:1368	arg1	coli					1441:1444	Escherichia coli	1429:1444	Escherichia coli	1429:1444	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	3	99	theme	Plumbago	444:451	arg1	extract					433:439	extract	433:439	extract of Plumbago zeylanica L	433:463	In this process, leaves extract of Plumbago zeylanica L was, used as a nucleating agent.					
33858076	8	100	theme	MgO	989:991	arg1	spectra					978:984	The photoluminescence (PL) spectra	951:984	The photoluminescence (PL) spectra	951:984	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	4	101	theme	cubic	541:545	arg1	structures					547:556	face-centered cubic structures	527:556	face-centered cubic structures	527:556	The MgO and CMgO NPs exhibit face-centered cubic structures, as confirmed by XRD studies.					
33858076	11	102	theme	MTT	1660:1662	arg1	assay					1664:1668	an MTT assay	1657:1668	an MTT assay	1657:1668	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
33858076	8	103	theme	CMgO	997:1000	arg1	spectra					978:984	The photoluminescence (PL) spectra	951:984	The photoluminescence (PL) spectra	951:984	The photoluminescence (PL) spectra of MgO and CMgO NPs, revealed oxygen vacancies at 499 nm and 519 nm, respectively, due to the active radicals generated, which were responsible for their biocidal activities.					
33858076	10	104	from	pneumoniae	1417:1426	arg1	activities					1370:1379	The antibacterial activities	1352:1379	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae	1352:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	104	from	pneumoniae	1417:1426	arg1	vulgaris					1501:1508	Proteus vulgaris	1493:1508	Proteus vulgaris	1493:1508	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	104	from	pneumoniae	1417:1426	arg1	dysenteriae					1456:1466	Shigella dysenteriae	1447:1466	Shigella dysenteriae	1447:1466	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	104	from	pneumoniae	1417:1426	arg1	aeruginosa					1481:1490	Pseudomonas aeruginosa	1469:1490	Pseudomonas aeruginosa	1469:1490	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	104	from	pneumoniae	1417:1426	arg1	cholerae					1521:1528	Vibrio cholerae	1514:1528	Vibrio cholerae	1514:1528	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	104	from	pneumoniae	1417:1426	arg1	coli					1441:1444	Escherichia coli	1429:1444	Escherichia coli	1429:1444	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	105	theme	nanoparticles	1388:1400	arg1	activities					1370:1379	The antibacterial activities	1352:1379	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae	1352:1426	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	105	theme	nanoparticles	1388:1400	arg1	vulgaris					1501:1508	Proteus vulgaris	1493:1508	Proteus vulgaris	1493:1508	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	105	theme	nanoparticles	1388:1400	arg1	dysenteriae					1456:1466	Shigella dysenteriae	1447:1466	Shigella dysenteriae	1447:1466	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	105	theme	nanoparticles	1388:1400	arg1	aeruginosa					1481:1490	Pseudomonas aeruginosa	1469:1490	Pseudomonas aeruginosa	1469:1490	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	105	theme	nanoparticles	1388:1400	arg1	cholerae					1521:1528	Vibrio cholerae	1514:1528	Vibrio cholerae	1514:1528	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	10	105	theme	nanoparticles	1388:1400	arg1	coli					1441:1444	Escherichia coli	1429:1444	Escherichia coli	1429:1444	The antibacterial activities of the nanoparticles on: Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus vulgaris and Vibrio cholerae bacteria, were studied with the well diffusion method.					
33858076	11	106	theme	CMgO	1689:1692	arg1	NPs					1694:1696	CMgO NPs	1689:1696	CMgO NPs	1689:1696	The MgO and CMgO NPs were tested on breast cancer cell line (MCF-7) via an MTT assay and it proved that CMgO NPs possess higher anticancer properties than MgO NPs.					
34062407	6	0	theme	exendin-4	882:890	arg1	peptide					899:905	the exendin-4 (Ex-4) peptide	878:905	the exendin-4 (Ex-4) peptide	878:905	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	0	theme	exendin-4	882:890	arg1	agonist					951:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	3	1	dep	strategies	390:399	arg1	promote					464:470	promote	464:470	to promote tendon repair and regeneration	461:501	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	10	2	dep	factors	1466:1472	arg1	Col-I					1514:1518	Col-I	1514:1518	Col-I	1514:1518	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	Tnc					1535:1537	Tnc	1535:1537	Tnc	1535:1537	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	Scx					1483:1485	Scx	1483:1485	Scx	1483:1485	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	Dcn					1521:1523	Dcn	1521:1523	Dcn	1521:1523	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	factors					1466:1472	tendon-related transcription factors	1437:1472	tendon-related transcription factors (Mkx and Scx)	1437:1486	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	genes					1540:1544	genes	1540:1544	genes	1540:1544	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	Bgn					1526:1528	Bgn	1526:1528	Bgn	1526:1528	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	2	dep	factors	1466:1472	arg1	Mkx					1475:1477	Mkx	1475:1477	Mkx	1475:1477	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	4	3	theme	molecules	571:579	arg1	form					557:560	the form	553:560	the form of small molecules, donor cells, and progenitors	553:609	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	8	4	theme	Ex-4	1184:1187	arg1	concentration					1189:1201	A 20 nM Ex-4 concentration	1176:1201	A 20 nM Ex-4 concentration	1176:1201	A 20 nM Ex-4 concentration was optimal for promoting cell proliferation and tendonogenic differentiation.					
34062407	8	4	theme	Ex-4	1184:1187	arg1	optimal					1207:1213	optimal	1207:1213	optimal	1207:1213	A 20 nM Ex-4 concentration was optimal for promoting cell proliferation and tendonogenic differentiation.					
34062407	2	5	theme	tendon	242:247	arg1	nature					228:233	The poor healing nature	211:233	The poor healing nature of the tendon	211:247	The poor healing nature of the tendon is attributed to poor vascularization and cellular composition.					
34062407	10	6	theme	proteins	1550:1557	arg1	levels					1427:1432	the levels	1423:1432	the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins	1423:1557	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	1	7	theme	musculoskeletal	164:178	arg1	injuries					180:187	musculoskeletal injuries	164:187	musculoskeletal injuries in the United States	164:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	6	8	theme	peptide	924:930	arg1	peptide					899:905	the exendin-4 (Ex-4) peptide	878:905	the exendin-4 (Ex-4) peptide	878:905	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	8	theme	peptide	924:930	arg1	agonist					951:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	4	9	theme	progenitors	599:609	arg1	form					557:560	the form	553:560	the form of small molecules, donor cells, and progenitors	553:609	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	5	10	theme	drug	683:686	arg1	molecules					688:696	Small drug molecules	677:696	Small drug molecules	677:696	Small drug molecules and peptides offer stability over growth factors that are known to suffer from relatively short biological half-lives.					
34062407	2	11	theme	poor	266:269	arg1	vascularization					271:285	poor vascularization	266:285	poor vascularization	266:285	The poor healing nature of the tendon is attributed to poor vascularization and cellular composition.					
34062407	4	12	theme	several	617:623	arg1	advantages					625:634	several advantages	617:634	several advantages	617:634	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	5	13	theme	biological	794:803	arg1	half-lives					805:814	relatively short biological half-lives	777:814	relatively short biological half-lives	777:814	Small drug molecules and peptides offer stability over growth factors that are known to suffer from relatively short biological half-lives.					
34062407	1	14	theme	United	196:201	arg1	States					203:208	the United States	192:208	the United States	192:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	6	15	theme	peptide	899:905	arg1	ability					867:873	the ability	863:873	the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs)	863:1053	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	0	16	from	tenogenesis	63:73	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	1	17	from	injuries	180:187	arg1	States					203:208	the United States	192:208	the United States	192:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	11	18	with	conjunction	1695:1705	arg1	hMSCs					1712:1716	hMSCs	1712:1716	hMSCs	1712:1716	The tendonogenic factor Ex-4 in conjunction with hMSCs appear to enhance tendon regeneration.					
34062407	4	19	theme	healing	659:665	arg1	response					667:674	the tendon healing response	648:674	the tendon healing response	648:674	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	2	20	theme	healing	220:226	arg1	nature					228:233	The poor healing nature	211:233	The poor healing nature of the tendon	211:247	The poor healing nature of the tendon is attributed to poor vascularization and cellular composition.					
34062407	11	21	from	Ex-4	1687:1690	arg1	conjunction					1695:1705	conjunction	1695:1705	conjunction with hMSCs	1695:1716	The tendonogenic factor Ex-4 in conjunction with hMSCs appear to enhance tendon regeneration.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	Col-I					1514:1518	Col-I	1514:1518	Col-I	1514:1518	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	Tnc					1535:1537	Tnc	1535:1537	Tnc	1535:1537	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	Scx					1483:1485	Scx	1483:1485	Scx	1483:1485	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	Dcn					1521:1523	Dcn	1521:1523	Dcn	1521:1523	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	factors					1466:1472	tendon-related transcription factors	1437:1472	tendon-related transcription factors (Mkx and Scx)	1437:1486	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	Bgn					1526:1528	Bgn	1526:1528	Bgn	1526:1528	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	22	theme	tendon-related	1437:1450	arg1	Mkx					1475:1477	Mkx	1475:1477	Mkx	1475:1477	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	11	23	theme	tendon	1736:1741	arg1	regeneration					1743:1754	tendon regeneration	1736:1754	tendon regeneration	1736:1754	The tendonogenic factor Ex-4 in conjunction with hMSCs appear to enhance tendon regeneration.					
34062407	0	24	theme	human	78:82	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	3	25	theme	FDA-approved	331:342	arg1	factors					351:357	FDA-approved growth factors	331:357	FDA-approved growth factors for tendon repair	331:375	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	7	26	theme	tendonogenic	1146:1157	arg1	differentiation					1159:1173	tendonogenic differentiation	1146:1173	tendonogenic differentiation	1146:1173	We treated hMSCs with varied doses of Ex-4 in culture media to evaluate proliferation and tendonogenic differentiation.					
34062407	0	27	theme	stem	96:99	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	10	28	theme	other	1608:1612	arg1	IGF-1					1645:1649	IGF-1	1645:1649	IGF-1	1645:1649	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	28	theme	other	1608:1612	arg1	insulin					1633:1639	insulin	1633:1639	insulin	1633:1639	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	28	theme	other	1608:1612	arg1	controls					1614:1621	other controls	1608:1621	other controls including insulin and IGF-1 treatments	1608:1660	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	6	29	theme	mesenchymal	1024:1034	arg1	hMSCs					1048:1052	hMSCs	1048:1052	hMSCs	1048:1052	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	29	theme	mesenchymal	1024:1034	arg1	cells					1041:1045	bone marrow-derived human mesenchymal stem cells	998:1045	bone marrow-derived human mesenchymal stem cells (hMSCs)	998:1053	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	3	30	theme	tendon	363:368	arg1	repair					370:375	tendon repair	363:375	tendon repair	363:375	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	10	31	theme	factors	1466:1472	arg1	levels					1427:1432	the levels	1423:1432	the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins	1423:1557	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	3	32	theme	bioactive	407:415	arg1	factors					417:423	bioactive factors	407:423	bioactive factors	407:423	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	5	33	theme	short	788:792	arg1	half-lives					805:814	relatively short biological half-lives	777:814	relatively short biological half-lives	777:814	Small drug molecules and peptides offer stability over growth factors that are known to suffer from relatively short biological half-lives.					
34062407	9	34	theme	hMSCs	1314:1318	arg1	differentiation					1295:1309	Tendonogenic differentiation	1282:1309	Tendonogenic differentiation of hMSCs	1282:1318	Tendonogenic differentiation of hMSCs was evaluated via gene expression profile, immunofluorescence, and biochemical analyses.					
34062407	6	35	theme	marrow-derived	1003:1016	arg1	hMSCs					1048:1052	hMSCs	1048:1052	hMSCs	1048:1052	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	35	theme	marrow-derived	1003:1016	arg1	cells					1041:1045	bone marrow-derived human mesenchymal stem cells	998:1045	bone marrow-derived human mesenchymal stem cells (hMSCs)	998:1053	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	3	36	theme	factors	351:357	arg1	absence					320:326	the absence	316:326	the absence of FDA-approved growth factors for tendon repair	316:375	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	4	37	theme	factor	530:535	arg1	alternatives					537:548	Growth factor alternatives	523:548	Growth factor alternatives in the form of small molecules, donor cells, and progenitors	523:609	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	0	38	theme	peptide	18:24	arg1	Exendin-4					45:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	1	39	theme	Tendon	108:113	arg1	injuries					115:122	Tendon injuries	108:122	Tendon injuries	108:122	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	0	40	theme	receptor	28:35	arg1	Exendin-4					45:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	9	41	theme	gene	1338:1341	arg1	profile					1354:1360	gene expression profile	1338:1360	gene expression profile	1338:1360	Tendonogenic differentiation of hMSCs was evaluated via gene expression profile, immunofluorescence, and biochemical analyses.					
34062407	6	42	theme	tenocyte	970:977	arg1	differentiation					979:993	tenocyte differentiation	970:993	tenocyte differentiation	970:993	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	1	43	dep	50	157:158	arg1	to					154:155	to	154:155	to	154:155	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	7	44	theme	varied	1078:1083	arg1	doses					1085:1089	varied doses	1078:1089	varied doses of Ex-4	1078:1097	We treated hMSCs with varied doses of Ex-4 in culture media to evaluate proliferation and tendonogenic differentiation.					
34062407	7	45	from	hMSCs	1067:1071	arg1	media					1110:1114	culture media	1102:1114	culture media	1102:1114	We treated hMSCs with varied doses of Ex-4 in culture media to evaluate proliferation and tendonogenic differentiation.					
34062407	10	46	theme	matrix	1506:1511	arg1	levels					1427:1432	the levels	1423:1432	the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins	1423:1557	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	11	47	theme	factor	1680:1685	arg1	Ex-4					1687:1690	The tendonogenic factor Ex-4	1663:1690	The tendonogenic factor Ex-4 in conjunction with hMSCs	1663:1716	The tendonogenic factor Ex-4 in conjunction with hMSCs appear to enhance tendon regeneration.					
34062407	4	48	theme	cells	588:592	arg1	form					557:560	the form	553:560	the form of small molecules, donor cells, and progenitors	553:609	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	6	49	link	marrow-derived	1003:1016	arg1	hMSCs					1048:1052	hMSCs	1048:1052	hMSCs	1048:1052	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	49	link	marrow-derived	1003:1016	arg1	cells					1041:1045	bone marrow-derived human mesenchymal stem cells	998:1045	bone marrow-derived human mesenchymal stem cells (hMSCs)	998:1053	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	7	50	with	hMSCs	1067:1071	arg1	doses					1085:1089	varied doses	1078:1089	varied doses of Ex-4	1078:1097	We treated hMSCs with varied doses of Ex-4 in culture media to evaluate proliferation and tendonogenic differentiation.					
34062407	4	51	theme	small	565:569	arg1	molecules					571:579	small molecules	565:579	small molecules	565:579	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	8	52	theme	20 nM	1178:1182	arg1	concentration					1189:1201	A 20 nM Ex-4 concentration	1176:1201	A 20 nM Ex-4 concentration	1176:1201	A 20 nM Ex-4 concentration was optimal for promoting cell proliferation and tendonogenic differentiation.					
34062407	8	52	theme	20 nM	1178:1182	arg1	optimal					1207:1213	optimal	1207:1213	optimal	1207:1213	A 20 nM Ex-4 concentration was optimal for promoting cell proliferation and tendonogenic differentiation.					
34062407	2	53	theme	cellular	291:298	arg1	composition					300:310	cellular composition	291:310	cellular composition	291:310	The poor healing nature of the tendon is attributed to poor vascularization and cellular composition.					
34062407	5	54	theme	Small	677:681	arg1	molecules					688:696	Small drug molecules	677:696	Small drug molecules	677:696	Small drug molecules and peptides offer stability over growth factors that are known to suffer from relatively short biological half-lives.					
34062407	1	55	theme	injuries	180:187	arg1	%					159:159	up to 50%	151:159	up to 50% of musculoskeletal injuries in the United States	151:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	1	55	theme	injuries	180:187	arg1	injuries					180:187	musculoskeletal injuries	164:187	musculoskeletal injuries in the United States	164:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	6	56	theme	glucagon-like	910:922	arg1	peptide					899:905	the exendin-4 (Ex-4) peptide	878:905	the exendin-4 (Ex-4) peptide	878:905	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	56	theme	glucagon-like	910:922	arg1	agonist					951:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	2	57	theme	poor	215:218	arg1	nature					228:233	The poor healing nature	211:233	The poor healing nature of the tendon	211:247	The poor healing nature of the tendon is attributed to poor vascularization and cellular composition.					
34062407	8	58	theme	cell	1229:1232	arg1	proliferation					1234:1246	cell proliferation	1229:1246	cell proliferation	1229:1246	A 20 nM Ex-4 concentration was optimal for promoting cell proliferation and tendonogenic differentiation.					
34062407	7	59	theme	Ex-4	1094:1097	arg1	doses					1085:1089	varied doses	1078:1089	varied doses of Ex-4	1078:1097	We treated hMSCs with varied doses of Ex-4 in culture media to evaluate proliferation and tendonogenic differentiation.					
34062407	1	60	from	States	203:208	arg1	%					159:159	up to 50%	151:159	up to 50% of musculoskeletal injuries in the United States	151:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	1	60	from	States	203:208	arg1	injuries					180:187	musculoskeletal injuries	164:187	musculoskeletal injuries in the United States	164:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	8	61	theme	tendonogenic	1252:1263	arg1	differentiation					1265:1279	tendonogenic differentiation	1252:1279	tendonogenic differentiation	1252:1279	A 20 nM Ex-4 concentration was optimal for promoting cell proliferation and tendonogenic differentiation.					
34062407	5	62	theme	growth	732:737	arg1	factors					739:745	growth factors	732:745	growth factors that are known to suffer from relatively short biological half-lives	732:814	Small drug molecules and peptides offer stability over growth factors that are known to suffer from relatively short biological half-lives.					
34062407	4	63	theme	tendon	652:657	arg1	response					667:674	the tendon healing response	648:674	the tendon healing response	648:674	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	0	64	theme	mesenchymal	84:94	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	1	65	from	%	159:159	arg1	States					203:208	the United States	192:208	the United States	192:208	Tendon injuries are common and account for up to 50% of musculoskeletal injuries in the United States.					
34062407	6	66	theme	receptor	942:949	arg1	peptide					899:905	the exendin-4 (Ex-4) peptide	878:905	the exendin-4 (Ex-4) peptide	878:905	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	66	theme	receptor	942:949	arg1	agonist					951:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	67	theme	GLP-1	935:939	arg1	peptide					899:905	the exendin-4 (Ex-4) peptide	878:905	the exendin-4 (Ex-4) peptide	878:905	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	67	theme	GLP-1	935:939	arg1	agonist					951:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	a glucagon-like peptide 1 (GLP-1) receptor agonist	908:957	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	3	68	theme	growth	344:349	arg1	factors					351:357	FDA-approved growth factors	331:357	FDA-approved growth factors for tendon repair	331:375	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	9	69	theme	Tendonogenic	1282:1293	arg1	differentiation					1295:1309	Tendonogenic differentiation	1282:1309	Tendonogenic differentiation of hMSCs	1282:1318	Tendonogenic differentiation of hMSCs was evaluated via gene expression profile, immunofluorescence, and biochemical analyses.					
34062407	6	70	theme	stem	1036:1039	arg1	hMSCs					1048:1052	hMSCs	1048:1052	hMSCs	1048:1052	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	70	theme	stem	1036:1039	arg1	cells					1041:1045	bone marrow-derived human mesenchymal stem cells	998:1045	bone marrow-derived human mesenchymal stem cells (hMSCs)	998:1053	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	3	71	theme	donor	426:430	arg1	cells					432:436	donor cells	426:436	donor cells	426:436	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	0	72	theme	glucagon-like	4:16	arg1	Exendin-4					45:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	6	73	theme	human	1018:1022	arg1	hMSCs					1048:1052	hMSCs	1048:1052	hMSCs	1048:1052	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	6	73	theme	human	1018:1022	arg1	cells					1041:1045	bone marrow-derived human mesenchymal stem cells	998:1045	bone marrow-derived human mesenchymal stem cells (hMSCs)	998:1053	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	10	74	theme	transcription	1452:1464	arg1	Col-I					1514:1518	Col-I	1514:1518	Col-I	1514:1518	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	74	theme	transcription	1452:1464	arg1	Tnc					1535:1537	Tnc	1535:1537	Tnc	1535:1537	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	74	theme	transcription	1452:1464	arg1	Scx					1483:1485	Scx	1483:1485	Scx	1483:1485	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	74	theme	transcription	1452:1464	arg1	Dcn					1521:1523	Dcn	1521:1523	Dcn	1521:1523	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	74	theme	transcription	1452:1464	arg1	factors					1466:1472	tendon-related transcription factors	1437:1472	tendon-related transcription factors (Mkx and Scx)	1437:1486	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	74	theme	transcription	1452:1464	arg1	Bgn					1526:1528	Bgn	1526:1528	Bgn	1526:1528	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	10	74	theme	transcription	1452:1464	arg1	Mkx					1475:1477	Mkx	1475:1477	Mkx	1475:1477	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	4	75	from	alternatives	537:548	arg1	form					557:560	the form	553:560	the form of small molecules, donor cells, and progenitors	553:609	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	3	76	theme	delivery	443:450	arg1	matrices					452:459	delivery matrices	443:459	delivery matrices	443:459	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	0	77	theme	agonist	37:43	arg1	Exendin-4					45:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4	0:53	The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.					
34062407	6	78	theme	study	843:847	arg1	focus					829:833	The primary focus	817:833	The primary focus of this study	817:847	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	9	79	theme	expression	1343:1352	arg1	profile					1354:1360	gene expression profile	1338:1360	gene expression profile	1338:1360	Tendonogenic differentiation of hMSCs was evaluated via gene expression profile, immunofluorescence, and biochemical analyses.					
34062407	3	80	theme	engineering	378:388	arg1	strategies					390:399	engineering strategies	378:399	engineering strategies using bioactive factors	378:423	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	4	81	theme	Growth	523:528	arg1	factor					530:535	Growth factor	523:535	Growth factor alternatives in the form of small molecules, donor cells, and progenitors	523:609	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	10	82	dep	insulin	1633:1639	arg1	treatments					1651:1660	treatments	1651:1660	treatments	1651:1660	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	3	83	theme	tendon	472:477	arg1	repair					479:484	tendon repair	472:484	tendon repair	472:484	In the absence of FDA-approved growth factors for tendon repair, engineering strategies using bioactive factors, donor cells, and delivery matrices to promote tendon repair and regeneration are being explored.					
34062407	6	84	theme	primary	821:827	arg1	focus					829:833	The primary focus	817:833	The primary focus of this study	817:847	The primary focus of this study was to assess the ability of the exendin-4 (Ex-4) peptide, a glucagon-like peptide 1 (GLP-1) receptor agonist, to induce tenocyte differentiation in bone marrow-derived human mesenchymal stem cells (hMSCs).					
34062407	4	85	theme	donor	582:586	arg1	cells					588:592	donor cells	582:592	donor cells	582:592	Growth factor alternatives in the form of small molecules, donor cells, and progenitors offer several advantages and enhance the tendon healing response.					
34062407	10	86	theme	extracellular	1492:1504	arg1	matrix					1506:1511	extracellular matrix	1492:1511	extracellular matrix	1492:1511	Collectively, the levels of tendon-related transcription factors (Mkx and Scx) and extracellular matrix (Col-I, Dcn, Bgn, and Tnc) genes and proteins were elevated compared to media without Ex-4 and other controls including insulin and IGF-1 treatments.					
34062407	11	87	theme	tendonogenic	1667:1678	arg1	Ex-4					1687:1690	The tendonogenic factor Ex-4	1663:1690	The tendonogenic factor Ex-4 in conjunction with hMSCs	1663:1716	The tendonogenic factor Ex-4 in conjunction with hMSCs appear to enhance tendon regeneration.					
34062407	7	88	theme	culture	1102:1108	arg1	media					1110:1114	culture media	1102:1114	culture media	1102:1114	We treated hMSCs with varied doses of Ex-4 in culture media to evaluate proliferation and tendonogenic differentiation.					
34062407	9	89	theme	biochemical	1387:1397	arg1	analyses					1399:1406	biochemical analyses	1387:1406	biochemical analyses	1387:1406	Tendonogenic differentiation of hMSCs was evaluated via gene expression profile, immunofluorescence, and biochemical analyses.					
32847778	11	0	theme	chemotaxonomic	1504:1517	arg1	analyses					1536:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	9	1	theme	glycoside	1256:1264	arg1	hydrolases					1266:1275	glycoside hydrolases	1256:1275	glycoside hydrolases	1256:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	11	2	theme	=	1583:1583	arg1	B-3467T					1600:1606	= CECT 9407T=VKM B-3467T	1583:1606	= CECT 9407T=VKM B-3467T	1583:1606	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	2	theme	=	1583:1583	arg1	PL17T					1576:1580	strain PL17T	1569:1580	strain PL17T (= CECT 9407T=VKM B-3467T)	1569:1607	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	3	theme	phenotypic	1492:1501	arg1	analyses					1536:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	9	4	theme	hydrolases	1266:1275	arg1	family					1246:1251	the GH5 family	1238:1251	the GH5 family of glycoside hydrolases	1238:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	11	5	theme	Gemmataceae	1665:1675	arg1	species					1643:1649	species	1643:1649	species	1643:1649	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	5	theme	Gemmataceae	1665:1675	arg1	genus					1633:1637	a novel genus	1625:1637	a novel genus	1625:1637	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	5	theme	Gemmataceae	1665:1675	arg1	nov.					1708:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	6	6	theme	major	834:838	arg1	C18:0					857:861	C18:0	857:861	C18:0	857:861	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	6	6	theme	major	834:838	arg1	acids					846:850	The major fatty acids	830:850	The major fatty acids	830:850	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	10	7	theme	%	1400:1400	arg1	similarity					1402:1411	94.5% similarity	1396:1411	94.5% similarity to that in Gemmata obscuriglobus UQM2246T	1396:1453	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	6	8	theme	polar	898:902	arg1	lipid					904:908	the major polar lipid	888:908	the major polar lipid	888:908	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	6	8	theme	polar	898:902	arg1	trimethylornithine					914:931	trimethylornithine	914:931	trimethylornithine	914:931	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	3	9	theme	growth	514:519	arg1	representative					400:413	a novel representative	392:413	a novel representative of this family	392:428	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	9	theme	growth	514:519	arg1	capable					503:509	capable	503:509	capable	503:509	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	2	10	theme	ecological	313:322	arg1	functions					324:332	their ecological functions	307:332	their ecological functions	307:332	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	4	11	theme	pink-pigmented	587:600	arg1	spheres					602:608	pink-pigmented spheres	587:608	pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates	587:689	Cells of this isolate were represented by pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates.					
32847778	9	12	theme	potential	1118:1126	arg1	genes					1143:1147	Over 8900 potential protein-coding genes	1108:1147	Over 8900 potential protein-coding genes	1108:1147	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	6	13	theme	major	892:896	arg1	lipid					904:908	the major polar lipid	888:908	the major polar lipid	888:908	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	6	13	theme	major	892:896	arg1	trimethylornithine					914:931	trimethylornithine	914:931	trimethylornithine	914:931	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	8	14	theme	G+C	1022:1024	arg1	contents					1026:1033	The G+C contents	1018:1033	The G+C contents of the chromosomal and plasmid DNA	1018:1068	The G+C contents of the chromosomal and plasmid DNA were 67.4 and 62.3mol%, respectively.					
32847778	8	14	theme	G+C	1022:1024	arg1	%					1091:1091	67.4 and 62.3mol%	1075:1091	67.4 and 62.3mol%	1075:1091	The G+C contents of the chromosomal and plasmid DNA were 67.4 and 62.3mol%, respectively.					
32847778	3	15	from	growth	514:519	arg1	cellulose					534:542	cellulose	534:542	cellulose	534:542	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	15	from	growth	514:519	arg1	xylan					524:528	xylan	524:528	xylan	524:528	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	11	16	theme	9407T=VKM	1590:1598	arg1	B-3467T					1600:1606	= CECT 9407T=VKM B-3467T	1583:1606	= CECT 9407T=VKM B-3467T	1583:1606	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	16	theme	9407T=VKM	1590:1598	arg1	PL17T					1576:1580	strain PL17T	1569:1580	strain PL17T (= CECT 9407T=VKM B-3467T)	1569:1607	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	2	17	from	sizes	224:228	arg1	environments					290:301	freshwater and terrestrial environments	263:301	environments	290:301	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	3	18	attach	isolated	455:462	arg2	PL17T					438:442	strain PL17T	431:442	strain PL17T	431:442	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	18	attach	isolated	455:462	arg2	capable					503:509	capable	503:509	capable	503:509	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	18	attach	isolated	455:462	arg1	wetland					487:493	a littoral tundra wetland	469:493	a littoral tundra wetland	469:493	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	18	attach	isolated	455:462	arg2	representative					400:413	a novel representative	392:413	a novel representative of this family	392:428	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	5	19	theme	chemoorganotrophic	748:765	arg1	bacterium					767:775	obligately aerobic, mildly acidophilic chemoorganotrophic bacterium	709:775	obligately aerobic, mildly acidophilic chemoorganotrophic bacterium	709:775	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	10	20	theme	94.5	1396:1399	arg1	%					1400:1400	%	1400:1400	%	1400:1400	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	2	21	theme	terrestrial	278:288	arg1	environments					290:301	freshwater and terrestrial environments	263:301	environments	290:301	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	0	22	theme	tundricola	14:23	arg1	nov.					30:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov., sp.					
32847778	11	23	theme	novel	1627:1631	arg1	genus					1633:1637	a novel genus	1625:1637	a novel genus	1625:1637	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	23	theme	novel	1627:1631	arg1	nov.					1708:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	0	24	theme	Frigoriglobus	0:12	arg1	nov.					30:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov., sp.					
32847778	8	25	theme	chromosomal	1042:1052	arg1	DNA					1066:1068	the chromosomal and plasmid DNA	1038:1068	DNA	1066:1068	The G+C contents of the chromosomal and plasmid DNA were 67.4 and 62.3mol%, respectively.					
32847778	9	26	theme	putative	1191:1198	arg1	cellulase					1200:1208	a putative cellulase	1189:1208	a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases	1189:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	11	27	theme	Frigoriglobus	1678:1690	arg1	sp					1714:1715	sp	1714:1715	sp	1714:1715	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	27	theme	Frigoriglobus	1678:1690	arg1	genus					1633:1637	a novel genus	1625:1637	a novel genus	1625:1637	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	27	theme	Frigoriglobus	1678:1690	arg1	nov.					1708:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	10	28	theme	strain	1292:1297	arg1	PL17T					1299:1303	strain PL17T	1292:1303	strain PL17T	1292:1303	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	4	29	theme	short	663:667	arg1	chains					669:674	short chains	663:674	short chains	663:674	Cells of this isolate were represented by pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates.					
32847778	7	30	theme	strain	948:953	arg1	PL17T					955:959	strain PL17T	948:959	strain PL17T	948:959	The genome of strain PL17T consisted of a 9.83 Mb chromosome and a 24.69kb plasmid.					
32847778	8	31	theme	DNA	1066:1068	arg1	contents					1026:1033	The G+C contents	1018:1033	The G+C contents of the chromosomal and plasmid DNA	1018:1068	The G+C contents of the chromosomal and plasmid DNA were 67.4 and 62.3mol%, respectively.					
32847778	8	31	theme	DNA	1066:1068	arg1	%					1091:1091	67.4 and 62.3mol%	1075:1091	67.4 and 62.3mol%	1075:1091	The G+C contents of the chromosomal and plasmid DNA were 67.4 and 62.3mol%, respectively.					
32847778	1	32	from	wetland	140:146	arg1	planctomycete					77:89	planctomycete	77:89	planctomycete	77:89	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	1	32	from	wetland	140:146	arg1	nov.					40:43	nov.	40:43	nov.	40:43	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	2	33	from	distribution	247:258	arg1	environments					290:301	freshwater and terrestrial environments	263:301	environments	290:301	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	0	34	theme	gen.	25:28	arg1	nov.					30:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov., sp.					
32847778	4	35	theme	isolate	559:565	arg1	Cells					545:549	Cells	545:549	Cells of this isolate	545:565	Cells of this isolate were represented by pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates.					
32847778	8	36	theme	plasmid	1058:1064	arg1	DNA					1066:1068	the chromosomal and plasmid DNA	1038:1068	DNA	1066:1068	The G+C contents of the chromosomal and plasmid DNA were 67.4 and 62.3mol%, respectively.					
32847778	2	37	theme	Gemmataceae	178:188	arg1	Planctomycetes					149:162	Planctomycetes	149:162	Planctomycetes of the family Gemmataceae	149:188	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	1	38	theme	family	98:103	arg1	Gemmataceae					105:115	the family Gemmataceae	94:115	the family Gemmataceae	94:115	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	7	39	theme	PL17T	955:959	arg1	genome					938:943	The genome	934:943	The genome of strain PL17T	934:959	The genome of strain PL17T consisted of a 9.83 Mb chromosome and a 24.69kb plasmid.					
32847778	3	40	theme	littoral	471:478	arg1	wetland					487:493	a littoral tundra wetland	469:493	a littoral tundra wetland	469:493	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	5	41	theme	good	794:797	arg1	tolerance					799:807	good tolerance	794:807	good tolerance of low temperatures	794:827	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	10	42	theme	rRNA	1365:1368	arg1	sequences					1375:1383	16S rRNA gene sequences	1361:1383	16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T	1361:1453	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	5	43	dep	bacterium	767:775	arg1	displayed					784:792	displayed	784:792	displayed good tolerance of low temperatures	784:827	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	2	44	theme	family	171:176	arg1	Gemmataceae					178:188	the family Gemmataceae	167:188	the family Gemmataceae	167:188	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	2	45	theme	cosmopolitan	234:245	arg1	distribution					247:258	cosmopolitan distribution	234:258	cosmopolitan distribution in freshwater and terrestrial environments	234:301	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	10	46	dep	one	1315:1317	arg1	operons					1348:1354	unlinked rRNA operons	1334:1354	unlinked rRNA operons	1334:1354	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	11	47	theme	phylogenomic	1523:1534	arg1	analyses					1536:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	3	48	theme	strain	431:436	arg1	representative					400:413	a novel representative	392:413	a novel representative of this family	392:428	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	48	theme	strain	431:436	arg1	PL17T					438:442	strain PL17T	431:442	strain PL17T	431:442	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	10	49	theme	unlinked	1334:1341	arg1	operons					1348:1354	unlinked rRNA operons	1334:1354	unlinked rRNA operons	1334:1354	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	5	50	theme	aerobic	720:726	arg1	bacterium					767:775	obligately aerobic, mildly acidophilic chemoorganotrophic bacterium	709:775	obligately aerobic, mildly acidophilic chemoorganotrophic bacterium	709:775	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	11	51	theme	CECT	1585:1588	arg1	B-3467T					1600:1606	= CECT 9407T=VKM B-3467T	1583:1606	= CECT 9407T=VKM B-3467T	1583:1606	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	51	theme	CECT	1585:1588	arg1	PL17T					1576:1580	strain PL17T	1569:1580	strain PL17T (= CECT 9407T=VKM B-3467T)	1569:1607	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	7	52	theme	24.69kb	1001:1007	arg1	plasmid					1009:1015	a 24.69kb plasmid	999:1015	a 24.69kb plasmid	999:1015	The genome of strain PL17T consisted of a 9.83 Mb chromosome and a 24.69kb plasmid.					
32847778	10	53	link	unlinked	1334:1341	arg1	operons					1348:1354	unlinked rRNA operons	1334:1354	unlinked rRNA operons	1334:1354	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	5	54	theme	low	812:814	arg1	temperatures					816:827	low temperatures	812:827	low temperatures	812:827	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	9	55	from	family	1246:1251	arg1	domain					1226:1231	a domain	1224:1231	a domain from the GH5 family of glycoside hydrolases	1224:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	0	56	dep	sp	36:37	arg1	nov.					30:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov.	0:33	Frigoriglobus tundricola gen. nov., sp.					
32847778	10	57	theme	rRNA	1343:1346	arg1	operons					1348:1354	unlinked rRNA operons	1334:1354	unlinked rRNA operons	1334:1354	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	1	58	theme	Gemmataceae	105:115	arg1	planctomycete					77:89	planctomycete	77:89	planctomycete	77:89	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	1	58	theme	Gemmataceae	105:115	arg1	nov.					40:43	nov.	40:43	nov.	40:43	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	3	59	theme	family	423:428	arg1	representative					400:413	a novel representative	392:413	a novel representative of this family	392:428	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	59	theme	family	423:428	arg1	PL17T					438:442	strain PL17T	431:442	strain PL17T	431:442	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	59	theme	family	423:428	arg1	capable					503:509	capable	503:509	capable	503:509	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	2	60	theme	large	211:215	arg1	sizes					224:228	large genome sizes	211:228	large genome sizes	211:228	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	2	61	theme	genome	217:222	arg1	sizes					224:228	large genome sizes	211:228	large genome sizes	211:228	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	11	62	theme	strain	1569:1574	arg1	B-3467T					1600:1606	= CECT 9407T=VKM B-3467T	1583:1606	= CECT 9407T=VKM B-3467T	1583:1606	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	62	theme	strain	1569:1574	arg1	PL17T					1576:1580	strain PL17T	1569:1580	strain PL17T (= CECT 9407T=VKM B-3467T)	1569:1607	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	10	63	contain	contained	1305:1313	arg1	genome					1282:1287	The genome	1278:1287	The genome of strain PL17T	1278:1303	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	10	63	contain	contained	1305:1313	arg2	one					1330:1332	one	1330:1332	one	1330:1332	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	10	63	contain	contained	1305:1313	arg2	one					1315:1317	one	1315:1317	one	1315:1317	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	10	64	theme	gene	1370:1373	arg1	sequences					1375:1383	16S rRNA gene sequences	1361:1383	16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T	1361:1453	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	11	65	theme	family	1658:1663	arg1	Gemmataceae					1665:1675	the family Gemmataceae	1654:1675	the family Gemmataceae	1654:1675	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	66	theme	tundricola	1692:1701	arg1	sp					1714:1715	sp	1714:1715	sp	1714:1715	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	66	theme	tundricola	1692:1701	arg1	genus					1633:1637	a novel genus	1625:1637	a novel genus	1625:1637	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	66	theme	tundricola	1692:1701	arg1	nov.					1708:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	67	theme	analyses	1536:1543	arg1	results					1469:1475	the results	1465:1475	the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses	1465:1543	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	10	68	theme	16S	1361:1363	arg1	sequences					1375:1383	16S rRNA gene sequences	1361:1383	16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T	1361:1453	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	3	69	theme	tundra	480:485	arg1	wetland					487:493	a littoral tundra wetland	469:493	a littoral tundra wetland	469:493	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	5	70	theme	Strain	692:697	arg1	PL17T					699:703	Strain PL17T	692:703	Strain PL17T	692:703	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	9	71	theme	GH5	1242:1244	arg1	family					1246:1251	the GH5 family	1238:1251	the GH5 family of glycoside hydrolases	1238:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	2	72	theme	freshwater	263:272	arg1	environments					290:301	freshwater and terrestrial environments	263:301	environments	290:301	Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood.					
32847778	7	73	theme	Mb	981:982	arg1	chromosome					984:993	a 9.83 Mb chromosome	974:993	a 9.83 Mb chromosome	974:993	The genome of strain PL17T consisted of a 9.83 Mb chromosome and a 24.69kb plasmid.					
32847778	1	74	theme	littoral	124:131	arg1	wetland					140:146	a littoral tundra wetland	122:146	a littoral tundra wetland	122:146	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	9	75	contain	contains	1215:1222	arg2	domain					1226:1231	a domain	1224:1231	a domain from the GH5 family of glycoside hydrolases	1224:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	9	75	contain	contains	1215:1222	arg1	cellulase					1200:1208	a putative cellulase	1189:1208	a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases	1189:1275	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	10	76	theme	PL17T	1299:1303	arg1	genome					1282:1287	The genome	1278:1287	The genome of strain PL17T	1278:1303	The genome of strain PL17T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246T.					
32847778	6	77	theme	fatty	840:844	arg1	C18:0					857:861	C18:0	857:861	C18:0	857:861	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	6	77	theme	fatty	840:844	arg1	acids					846:850	The major fatty acids	830:850	The major fatty acids	830:850	The major fatty acids were C18:0, C16:1ω5, and βOH-C16:1; the major polar lipid was trimethylornithine.					
32847778	9	78	theme	protein-coding	1128:1141	arg1	genes					1143:1147	Over 8900 potential protein-coding genes	1108:1147	Over 8900 potential protein-coding genes	1108:1147	Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases.					
32847778	1	79	theme	tundra	133:138	arg1	wetland					140:146	a littoral tundra wetland	122:146	a littoral tundra wetland	122:146	nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland.					
32847778	11	80	theme	gen.	1703:1706	arg1	sp					1714:1715	sp	1714:1715	sp	1714:1715	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	80	theme	gen.	1703:1706	arg1	genus					1633:1637	a novel genus	1625:1637	a novel genus	1625:1637	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	11	80	theme	gen.	1703:1706	arg1	nov.					1708:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Frigoriglobus tundricola gen. nov.	1678:1711	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32847778	5	81	dep	aerobic	720:726	arg1	acidophilic					736:746	acidophilic	736:746	acidophilic	736:746	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	3	82	theme	novel	394:398	arg1	representative					400:413	a novel representative	392:413	a novel representative of this family	392:428	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	82	theme	novel	394:398	arg1	PL17T					438:442	strain PL17T	431:442	strain PL17T	431:442	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	3	82	theme	novel	394:398	arg1	capable					503:509	capable	503:509	capable	503:509	In this study, we characterized a novel representative of this family, strain PL17T, which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose.					
32847778	5	83	theme	temperatures	816:827	arg1	tolerance					799:807	good tolerance	794:807	good tolerance of low temperatures	794:827	Strain PL17T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures.					
32847778	11	84	theme	comparative	1480:1490	arg1	analyses					1536:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	comparative phenotypic, chemotaxonomic and phylogenomic analyses	1480:1543	Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17T (= CECT 9407T=VKM B-3467T) as representing a novel genus and species of the family Gemmataceae, Frigoriglobus tundricola gen. nov., sp.					
32279739	12	0	from	application	1288:1298	arg1	site					1324:1327	the wound site	1314:1327	the wound site	1314:1327	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	12	1	theme	gel	1307:1309	arg1	application					1288:1298	Topical application	1280:1298	Topical application of the gel on the wound site	1280:1327	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	13	2	theme	better	1485:1490	arg1	proliferation					1499:1511	better tissue proliferation	1485:1511	better tissue proliferation (i.e. epithelialization)	1485:1536	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	13	2	theme	better	1485:1490	arg1	epithelialization					1519:1535	epithelialization	1519:1535	epithelialization	1519:1535	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	7	3	theme	hydrogel	779:786	arg1	AA-Ag-FA					789:796	agar-silver hydrogel (AA-Ag-FA);	767:798	agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM	767:866	Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM.					
32279739	12	4	from	rate	1360:1363	arg1	wound					1339:1343	the wound	1335:1343	the wound at much faster rate	1335:1363	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	15	5	theme	live-dead	1878:1886	arg1	staining					1893:1900	live-dead cell staining	1878:1900	live-dead cell staining	1878:1900	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature.					
32279739	2	6	theme	healing	258:264	arg1	mechanism					266:274	healing mechanism	258:274	healing mechanism	258:274	Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism.					
32279739	12	7	theme	Colloidal	1416:1424	arg1	hydrogel					1440:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	4	8	theme	hydration	368:376	arg1	debridement					418:428	enhanced autolytic debridement	399:428	enhanced autolytic debridement	399:428	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	4	8	theme	hydration	368:376	arg1	advantages					346:355	The advantages	342:355	The advantages of optimum hydration in wound healing	342:393	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	12	9	theme	32 ppm	1433:1438	arg1	hydrogel					1440:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	16	10	dep	antioxidant	2118:2128	arg1	the					2114:2116	the	2114:2116	the	2114:2116	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	11	theme	hydrogel	1953:1960	arg1	scaffold					1962:1969	our hydrogel scaffold	1949:1969	our hydrogel scaffold	1949:1969	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	4	12	from	advantages	346:355	arg1	healing					387:393	wound healing	381:393	wound healing	381:393	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	13	13	theme	collagen	1616:1623	arg1	bundles					1625:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	4	14	theme	cell	460:463	arg1	proliferation					465:477	accelerated cell proliferation	448:477	accelerated cell proliferation	448:477	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	3	15	theme	such	281:284	arg1	factor					296:301	One such extrinsic factor	277:301	One such extrinsic factor	277:301	One such extrinsic factor is moist environment in wound healing.					
32279739	4	16	theme	autolytic	408:416	arg1	debridement					418:428	enhanced autolytic debridement	399:428	enhanced autolytic debridement	399:428	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	4	16	theme	autolytic	408:416	arg1	advantages					346:355	The advantages	342:355	The advantages of optimum hydration in wound healing	342:393	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	14	17	theme	wound	1660:1664	arg1	tissue					1666:1671	wound tissue	1660:1671	wound tissue estimated through malondialdehyde (MDA) assay	1660:1717	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	6	18	theme	optimum	683:689	arg1	microenvironment					697:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment.					
32279739	8	19	theme	surface	873:879	arg1	morphology					881:890	The surface morphology	869:890	The surface morphology	869:890	The surface morphology is evaluated through SEM.					
32279739	13	20	dep	epithelialization	1519:1535	arg1	i.e.					1514:1517	i.e.	1514:1517	i.e.	1514:1517	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	6	21	theme	anti-inflammatory	661:677	arg1	microenvironment					697:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment.					
32279739	0	22	theme	multifunctional	58:72	arg1	avenue					74:79	A multifunctional avenue	56:79	A multifunctional avenue to tackle wound healing	56:103	Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing.					
32279739	3	23	theme	moist	306:310	arg1	environment					312:322	moist environment	306:322	moist environment in wound healing	306:339	One such extrinsic factor is moist environment in wound healing.					
32279739	6	24	theme	antimicrobial	646:658	arg1	microenvironment					697:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment.					
32279739	5	25	theme	lipid	602:606	arg1	peroxidation					608:619	tissue lipid peroxidation	595:619	tissue lipid peroxidation	595:619	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	1	26	theme	major	144:148	arg1	health					150:155	the major health	140:155	the major health concerns throughout the globe	140:185	Wound and its treatment is one of the major health concerns throughout the globe.					
32279739	0	27	theme	wound	91:95	arg1	healing					97:103	wound healing	91:103	wound healing	91:103	Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing.					
32279739	11	28	theme	DPPH	1267:1270	arg1	assay					1273:1277	2,2-diphenyl-1-picrylhydrazyl (DPPH) assay	1236:1277	2,2-diphenyl-1-picrylhydrazyl (DPPH) assay	1236:1277	The gel shows reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.					
32279739	13	29	theme	granulation	1544:1554	arg1	formation					1563:1571	more granulation tissue formation	1539:1571	more granulation tissue formation	1539:1571	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	11	30	theme	2,2-diphenyl-1-picrylhydrazyl	1236:1264	arg1	assay					1273:1277	2,2-diphenyl-1-picrylhydrazyl (DPPH) assay	1236:1277	2,2-diphenyl-1-picrylhydrazyl (DPPH) assay	1236:1277	The gel shows reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.					
32279739	13	31	theme	formation	1563:1571	arg1	bundles					1625:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	0	32	theme	Fumaric	0:6	arg1	acid					8:11	Fumaric acid	0:11	Fumaric acid	0:11	Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing.					
32279739	15	33	theme	samples	1841:1847	arg1	Cytotoxicity					1821:1832	Cytotoxicity	1821:1832	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining	1821:1900	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature.					
32279739	16	34	theme	acid	2098:2101	arg1	angiogenesis					2134:2145	angiogenesis	2134:2145	angiogenesis	2134:2145	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	34	theme	acid	2098:2101	arg1	antioxidant					2118:2128	antioxidant	2118:2128	antioxidant	2118:2128	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	34	theme	acid	2098:2101	arg1	NPs					2040:2042	the Ag NPs	2033:2042	the Ag NPs inside the gel	2033:2057	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	34	theme	acid	2098:2101	arg1	facilities					2103:2112	fumaric acid facilities	2090:2112	fumaric acid facilities	2090:2112	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	12	35	dep	heal	1397:1400	arg1	hydrogel					1440:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	0	36	theme	hybrid	39:44	arg1	hydrogel					46:53	Ag/agar-agar hybrid hydrogel	26:53	Ag/agar-agar hybrid hydrogel	26:53	Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing.					
32279739	7	37	theme	UV-Visible	817:826	arg1	spectroscopy					828:839	UV-Visible spectroscopy	817:839	UV-Visible spectroscopy	817:839	Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM.					
32279739	16	38	theme	bio-degradable	1976:1989	arg1	agar-agar					1991:1999	the bio-degradable agar-agar	1972:1999	the bio-degradable agar-agar	1972:1999	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	1	39	dep	health	150:155	arg1	concerns					157:164	concerns	157:164	concerns throughout the globe	157:185	Wound and its treatment is one of the major health concerns throughout the globe.					
32279739	13	40	theme	tissue	1492:1497	arg1	proliferation					1499:1511	better tissue proliferation	1485:1511	better tissue proliferation (i.e. epithelialization)	1485:1536	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	13	40	theme	tissue	1492:1497	arg1	epithelialization					1519:1535	epithelialization	1519:1535	epithelialization	1519:1535	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	9	41	theme	silver	934:939	arg1	NPs					959:961	Ag NPs	956:961	Ag NPs	956:961	The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm.					
32279739	9	41	theme	silver	934:939	arg1	nanoparticles					941:953	the silver nanoparticles	930:953	the silver nanoparticles (Ag NPs)	930:962	The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm.					
32279739	14	42	dep	treated	1756:1762	arg1	compared					1787:1794	compared	1787:1794	compared to standard (Mega heal)	1787:1818	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	10	43	theme	antibacterial	1019:1031	arg1	effect					1033:1038	potential antibacterial effect	1009:1038	potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection	1009:1169	The hydrogel shows potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection.					
32279739	12	44	theme	wound	1318:1322	arg1	site					1324:1327	the wound site	1314:1327	the wound site	1314:1327	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	15	45	theme	nontoxic	1912:1919	arg1	nature					1938:1943	its nontoxic biocompatibility nature	1908:1943	its nontoxic biocompatibility nature	1908:1943	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature.					
32279739	13	46	theme	Histological	1455:1466	arg1	analysis					1468:1475	Histological analysis	1455:1475	Histological analysis	1455:1475	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	11	47	theme	antioxidant	1197:1207	arg1	property					1209:1216	reasonable antioxidant property	1186:1216	reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay	1186:1277	The gel shows reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.					
32279739	7	48	theme	agar-silver	767:777	arg1	AA-Ag-FA					789:796	agar-silver hydrogel (AA-Ag-FA);	767:798	agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM	767:866	Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM.					
32279739	15	49	theme	cell	1888:1891	arg1	staining					1893:1900	live-dead cell staining	1878:1900	live-dead cell staining	1878:1900	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature.					
32279739	4	50	theme	wound	381:385	arg1	healing					387:393	wound healing	381:393	wound healing	381:393	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	4	51	theme	enhanced	399:406	arg1	debridement					418:428	enhanced autolytic debridement	399:428	enhanced autolytic debridement	399:428	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	4	51	theme	enhanced	399:406	arg1	advantages					346:355	The advantages	342:355	The advantages of optimum hydration in wound healing	342:393	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	2	52	theme	mechanism	266:274	arg1	dynamics					246:253	the dynamics	242:253	the dynamics of healing mechanism	242:274	Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism.					
32279739	5	53	theme	hydrated	507:514	arg1	wounds					516:521	hydrated wounds	507:521	hydrated wounds	507:521	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	1	54	theme	health	150:155	arg1	health					150:155	the major health	140:155	the major health concerns throughout the globe	140:185	Wound and its treatment is one of the major health concerns throughout the globe.					
32279739	1	54	theme	health	150:155	arg1	one					133:135	one	133:135	one	133:135	Wound and its treatment is one of the major health concerns throughout the globe.					
32279739	4	55	theme	optimum	360:366	arg1	hydration					368:376	optimum hydration	360:376	optimum hydration	360:376	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	6	56	theme	Healing	622:628	arg1	process					630:636	Healing process	622:636	Healing process	622:636	Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment.					
32279739	4	57	theme	accelerated	448:458	arg1	proliferation					465:477	accelerated cell proliferation	448:477	accelerated cell proliferation	448:477	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	12	58	theme	silver	1426:1431	arg1	hydrogel					1440:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Colloidal silver 32 ppm hydrogel	1416:1447	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	14	59	theme	AA-Ag-FA	1769:1776	arg1	hydrogel					1778:1785	AA-Ag-FA hydrogel	1769:1785	AA-Ag-FA hydrogel	1769:1785	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	13	60	theme	mature	1609:1614	arg1	collagen					1616:1623	mature collagen	1609:1623	mature collagen	1609:1623	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	12	61	theme	Mega	1392:1395	arg1	heal					1397:1400	Mega heal	1392:1400	Mega heal	1392:1400	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	12	61	theme	Mega	1392:1395	arg1	Composition					1403:1413	Composition	1403:1413	Composition	1403:1413	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	2	62	theme	intrinsic	210:218	arg1	factors					220:226	Various extrinsic and intrinsic factors	188:226	Various extrinsic and intrinsic factors	188:226	Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism.					
32279739	2	63	theme	Various	188:194	arg1	factors					220:226	Various extrinsic and intrinsic factors	188:226	Various extrinsic and intrinsic factors	188:226	Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism.					
32279739	14	64	theme	tissue	1666:1671	arg1	peroxidation					1644:1655	The lipid peroxidation	1634:1655	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay	1634:1717	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	14	64	theme	tissue	1666:1671	arg1	less					1746:1749	less	1746:1749	less	1746:1749	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	6	65	theme	moist	691:695	arg1	microenvironment					697:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	antimicrobial, anti-inflammatory and optimum moist microenvironment	646:712	Healing process prefers antimicrobial, anti-inflammatory and optimum moist microenvironment.					
32279739	3	66	from	environment	312:322	arg1	healing					333:339	wound healing	327:339	wound healing	327:339	One such extrinsic factor is moist environment in wound healing.					
32279739	13	67	theme	neovascularisation	1574:1591	arg1	bundles					1625:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	5	68	theme	associated	569:578	arg1	infection					580:588	associated infection	569:588	associated infection	569:588	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	16	69	dep	NPs	2040:2042	arg1	inside					2044:2049	inside	2044:2049	inside the gel	2044:2057	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	3	70	theme	extrinsic	286:294	arg1	factor					296:301	One such extrinsic factor	277:301	One such extrinsic factor	277:301	One such extrinsic factor is moist environment in wound healing.					
32279739	13	71	theme	fibroblast	1594:1603	arg1	bundles					1625:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	5	72	with	end	529:531	arg1	peroxidation					608:619	tissue lipid peroxidation	595:619	tissue lipid peroxidation	595:619	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	5	72	with	end	529:531	arg1	infection					580:588	associated infection	569:588	associated infection	569:588	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	5	72	with	end	529:531	arg1	uncomfortability					551:566	patient's uncomfortability	541:566	patient's uncomfortability	541:566	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	3	73	theme	wound	327:331	arg1	healing					333:339	wound healing	327:339	wound healing	327:339	One such extrinsic factor is moist environment in wound healing.					
32279739	12	74	theme	standard	1382:1389	arg1	gel					1450:1452	standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel	1382:1452	standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel	1382:1452	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	14	75	theme	lipid	1638:1642	arg1	peroxidation					1644:1655	The lipid peroxidation	1634:1655	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay	1634:1717	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	14	75	theme	lipid	1638:1642	arg1	less					1746:1749	less	1746:1749	less	1746:1749	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	12	76	theme	Topical	1280:1286	arg1	application					1288:1298	Topical application	1280:1298	Topical application of the gel on the wound site	1280:1327	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	16	77	theme	moist	2014:2018	arg1	environment					2020:2030	the moist environment	2010:2030	the moist environment	2010:2030	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	4	78	theme	collagen	483:490	arg1	formation					492:500	collagen formation	483:500	collagen formation	483:500	The advantages of optimum hydration in wound healing are enhanced autolytic debridement, angiogenesis and accelerated cell proliferation and collagen formation.					
32279739	16	79	theme	Ag	2037:2038	arg1	angiogenesis					2134:2145	angiogenesis	2134:2145	angiogenesis	2134:2145	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	79	theme	Ag	2037:2038	arg1	antioxidant					2118:2128	antioxidant	2118:2128	antioxidant	2118:2128	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	79	theme	Ag	2037:2038	arg1	NPs					2040:2042	the Ag NPs	2033:2042	the Ag NPs inside the gel	2033:2057	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	79	theme	Ag	2037:2038	arg1	facilities					2103:2112	fumaric acid facilities	2090:2112	fumaric acid facilities	2090:2112	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	15	80	theme	MTT	1864:1866	arg1	assay					1868:1872	MTT assay	1864:1872	MTT assay	1864:1872	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature.					
32279739	10	81	theme	wound	1155:1159	arg1	infection					1161:1169	wound infection	1155:1169	wound infection	1155:1169	The hydrogel shows potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection.					
32279739	12	82	theme	faster	1353:1358	arg1	rate					1360:1363	much faster rate	1348:1363	much faster rate	1348:1363	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	12	83	dep	standard	1382:1389	arg1	heal					1397:1400	Mega heal	1392:1400	Mega heal	1392:1400	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	12	83	dep	standard	1382:1389	arg1	Composition					1403:1413	Composition	1403:1413	Composition	1403:1413	Topical application of the gel on the wound site heals the wound at much faster rate even compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel) gel.					
32279739	14	84	theme	malondialdehyde	1691:1705	arg1	assay					1713:1717	malondialdehyde (MDA) assay	1691:1717	malondialdehyde (MDA) assay	1691:1717	The lipid peroxidation of wound tissue estimated through malondialdehyde (MDA) assay was found to be reasonably less when treated with AA-Ag-FA hydrogel compared to standard (Mega heal).					
32279739	7	85	theme	fumaric	741:747	arg1	acid					749:752	fumaric acid	741:752	fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM	741:866	Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM.					
32279739	0	86	theme	Ag/agar-agar	26:37	arg1	hydrogel					46:53	Ag/agar-agar hybrid hydrogel	26:53	Ag/agar-agar hybrid hydrogel	26:53	Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional avenue to tackle wound healing.					
32279739	10	87	theme	potential	1009:1017	arg1	effect					1033:1038	potential antibacterial effect	1009:1038	potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection	1009:1169	The hydrogel shows potential antibacterial effect against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are predominantly responsible for wound infection.					
32279739	5	88	theme	tissue	595:600	arg1	peroxidation					608:619	tissue lipid peroxidation	595:619	tissue lipid peroxidation	595:619	But hydrated wounds often end up with patient's uncomfortability, associated infection, and tissue lipid peroxidation.					
32279739	16	89	theme	bactericidal	2067:2078	arg1	agent					2080:2084	bactericidal agent	2067:2084	bactericidal agent	2067:2084	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	7	90	theme	FTIR	842:845	arg1	spectroscopy					847:858	FTIR spectroscopy	842:858	FTIR spectroscopy	842:858	Here, we have synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA); characterized by UV-Visible spectroscopy, FTIR spectroscopy and TEM.					
32279739	16	91	theme	fumaric	2090:2096	arg1	angiogenesis					2134:2145	angiogenesis	2134:2145	angiogenesis	2134:2145	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	91	theme	fumaric	2090:2096	arg1	antioxidant					2118:2128	antioxidant	2118:2128	antioxidant	2118:2128	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	91	theme	fumaric	2090:2096	arg1	NPs					2040:2042	the Ag NPs	2033:2042	the Ag NPs inside the gel	2033:2057	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	16	91	theme	fumaric	2090:2096	arg1	facilities					2103:2112	fumaric acid facilities	2090:2112	fumaric acid facilities	2090:2112	In our hydrogel scaffold, the bio-degradable agar-agar provides the moist environment; the Ag NPs inside the gel acts as bactericidal agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.					
32279739	15	92	theme	biocompatibility	1921:1936	arg1	nature					1938:1943	its nontoxic biocompatibility nature	1908:1943	its nontoxic biocompatibility nature	1908:1943	Cytotoxicity of the samples tested through MTT assay and live-dead cell staining shows its nontoxic biocompatibility nature.					
32279739	2	93	theme	extrinsic	196:204	arg1	factors					220:226	Various extrinsic and intrinsic factors	188:226	Various extrinsic and intrinsic factors	188:226	Various extrinsic and intrinsic factors can influence the dynamics of healing mechanism.					
32279739	13	94	theme	proliferation	1499:1511	arg1	bundles					1625:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles	1485:1631	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	9	95	theme	nanoparticles	941:953	arg1	10-15 nm					980:987	10-15 nm	980:987	10-15 nm	980:987	The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm.					
32279739	9	95	theme	nanoparticles	941:953	arg1	size					922:925	The size	918:925	The size of the silver nanoparticles (Ag NPs)	918:962	The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm.					
32279739	11	96	theme	reasonable	1186:1195	arg1	property					1209:1216	reasonable antioxidant property	1186:1216	reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay	1186:1277	The gel shows reasonable antioxidant property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.					
32279739	13	97	theme	tissue	1556:1561	arg1	formation					1563:1571	more granulation tissue formation	1539:1571	more granulation tissue formation	1539:1571	Histological analysis reveals better tissue proliferation (i.e. epithelialization), more granulation tissue formation, neovascularisation, fibroblast and mature collagen bundles.					
32279739	9	98	theme	Ag	956:957	arg1	NPs					959:961	Ag NPs	956:961	Ag NPs	956:961	The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm.					
32279739	9	98	theme	Ag	956:957	arg1	nanoparticles					941:953	the silver nanoparticles	930:953	the silver nanoparticles (Ag NPs)	930:962	The size of the silver nanoparticles (Ag NPs) was found to be 10-15 nm.					
32285052	4	0	theme	family	1000:1005	arg1	domain					1013:1018	(NOD)-like receptor family pyrin domain	980:1018	(NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation	980:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	3	1	theme	pathological	708:719	arg1	damage					721:726	colonic pathological damage	700:726	colonic pathological damage	700:726	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	5	2	theme	colitis	1182:1188	arg1	integrity					1169:1177	the intestinal barrier integrity	1146:1177	the intestinal barrier integrity of colitis	1146:1188	Moreover, the LRE profoundly ameliorated aberrant oxidative stress and restored the intestinal barrier integrity of colitis.					
32285052	4	3	theme	T	943:943	arg1	subsets					956:962	T lymphocyte subsets	943:962	T lymphocyte subsets	943:962	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	5	4	theme	intestinal	1150:1159	arg1	integrity					1169:1177	the intestinal barrier integrity	1146:1177	the intestinal barrier integrity of colitis	1146:1188	Moreover, the LRE profoundly ameliorated aberrant oxidative stress and restored the intestinal barrier integrity of colitis.					
32285052	4	5	theme	immune	881:886	arg1	homeostasis					888:898	intestinal immune homeostasis	870:898	intestinal immune homeostasis	870:898	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	1	6	theme	mice	420:423	arg1	model					425:429	experimental colitis mice model	399:429	experimental colitis mice model	399:429	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	7	theme	phytochemical	167:179	arg1	compositions					181:192	phytochemical compositions	167:192	phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE)	167:242	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	3	8	theme	activity	647:654	arg1	increase					662:669	disease activity index increase	639:669	disease activity index increase	639:669	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	0	9	theme	sulfate	100:106	arg1	colitis					143:149	dextran sulfate sodium-induced murine experimental colitis	92:149	dextran sulfate sodium-induced murine experimental colitis	92:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	5	10	theme	barrier	1161:1167	arg1	integrity					1169:1177	the intestinal barrier integrity	1146:1177	the intestinal barrier integrity of colitis	1146:1188	Moreover, the LRE profoundly ameliorated aberrant oxidative stress and restored the intestinal barrier integrity of colitis.					
32285052	6	11	theme	functional	1299:1308	arg1	IBD					1290:1292	IBD	1290:1292	IBD	1290:1292	Together, LRE supplementation might provide a new dietary strategy for preventing and ameliorating IBD as a functional food.					
32285052	6	11	theme	functional	1299:1308	arg1	food					1310:1313	a functional food	1297:1313	a functional food	1297:1313	Together, LRE supplementation might provide a new dietary strategy for preventing and ameliorating IBD as a functional food.					
32285052	6	12	theme	new	1237:1239	arg1	strategy					1249:1256	a new dietary strategy	1235:1256	a new dietary strategy for preventing and ameliorating IBD as a functional food	1235:1313	Together, LRE supplementation might provide a new dietary strategy for preventing and ameliorating IBD as a functional food.					
32285052	4	13	theme	receptor	991:998	arg1	domain					1013:1018	(NOD)-like receptor family pyrin domain	980:1018	(NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation	980:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	0	14	theme	dextran	92:98	arg1	sulfate					100:106	dextran sulfate	92:106	dextran sulfate sodium-induced murine experimental colitis	92:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	5	15	theme	aberrant	1107:1114	arg1	stress					1126:1131	aberrant oxidative stress	1107:1131	aberrant oxidative stress	1107:1131	Moreover, the LRE profoundly ameliorated aberrant oxidative stress and restored the intestinal barrier integrity of colitis.					
32285052	6	16	theme	LRE	1201:1203	arg1	supplementation					1205:1219	LRE supplementation	1201:1219	LRE supplementation	1201:1219	Together, LRE supplementation might provide a new dietary strategy for preventing and ameliorating IBD as a functional food.					
32285052	4	17	theme	nuclear	781:787	arg1	factor-κB					789:797	nuclear factor-κB	781:797	nuclear factor-κB (NF-κB)	781:805	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	17	theme	nuclear	781:787	arg1	NF-κB					800:804	NF-κB	800:804	NF-κB	800:804	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	0	18	theme	murine	123:128	arg1	colitis					143:149	dextran sulfate sodium-induced murine experimental colitis	92:149	dextran sulfate sodium-induced murine experimental colitis	92:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	5	19	theme	oxidative	1116:1124	arg1	stress					1126:1131	aberrant oxidative stress	1107:1131	aberrant oxidative stress	1107:1131	Moreover, the LRE profoundly ameliorated aberrant oxidative stress and restored the intestinal barrier integrity of colitis.					
32285052	0	20	theme	sodium-induced	108:121	arg1	colitis					143:149	dextran sulfate sodium-induced murine experimental colitis	92:149	dextran sulfate sodium-induced murine experimental colitis	92:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	4	21	theme	NOD	981:983	arg1	domain					1013:1018	(NOD)-like receptor family pyrin domain	980:1018	(NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation	980:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	3	22	theme	index	656:660	arg1	increase					662:669	disease activity index increase	639:669	disease activity index increase	639:669	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	1	23	theme	dextran	362:368	arg1	DSS					386:388	DSS	386:388	DSS	386:388	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	23	theme	dextran	362:368	arg1	sodium					378:383	a dextran sulfate sodium	360:383	a dextran sulfate sodium (DSS) induced experimental colitis mice model	360:429	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	4	24	theme	intestinal	870:879	arg1	homeostasis					888:898	intestinal immune homeostasis	870:898	intestinal immune homeostasis	870:898	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	2	25	theme	compounds	471:479	arg1	total					458:462	a total	456:462	a total of 129 compounds	456:479	The results showed that a total of 129 compounds were tentatively identified, including phenols/phenolic acids, flavonoids and others.					
32285052	1	26	theme	Lycium	197:202	arg1	LRE					239:241	LRE	239:241	LRE	239:241	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	26	theme	Lycium	197:202	arg1	extract					230:236	Lycium ruthenicum Murray ethanol extract	197:236	Lycium ruthenicum Murray ethanol extract (LRE)	197:242	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	0	27	theme	Dietary	0:6	arg1	intake					8:13	Dietary intake	0:13	Dietary intake of Lycium ruthenicum Murray ethanol extract	0:57	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	1	28	theme	protective	282:291	arg1	effect					293:298	the protective effect	278:298	the protective effect of LRE on inflammatory bowel disease (IBD)	278:341	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	29	from	effect	293:298	arg1	IBD					338:340	IBD	338:340	IBD	338:340	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	29	from	effect	293:298	arg1	disease					329:335	inflammatory bowel disease	310:335	inflammatory bowel disease (IBD)	310:341	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	4	30	theme	intestinal	743:752	arg1	inflammation					754:765	intestinal inflammation	743:765	intestinal inflammation	743:765	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	1	31	theme	ruthenicum	204:213	arg1	LRE					239:241	LRE	239:241	LRE	239:241	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	31	theme	ruthenicum	204:213	arg1	extract					230:236	Lycium ruthenicum Murray ethanol extract	197:236	Lycium ruthenicum Murray ethanol extract (LRE)	197:242	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	0	32	theme	Lycium	18:23	arg1	extract					51:57	Lycium ruthenicum Murray ethanol extract	18:57	Lycium ruthenicum Murray ethanol extract	18:57	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	4	33	theme	protein	829:835	arg1	MAPK					845:848	MAPK	845:848	MAPK	845:848	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	33	theme	protein	829:835	arg1	kinase					837:842	mitogen-activated protein kinase	811:842	mitogen-activated protein kinase (MAPK) pathways	811:858	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	34	theme	lymphocyte	945:954	arg1	subsets					956:962	T lymphocyte subsets	943:962	T lymphocyte subsets	943:962	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	35	contain	containing	1020:1029	arg2	activation					1054:1063	3 (NLRP3) inflammasome activation	1031:1063	3 (NLRP3) inflammasome activation	1031:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	35	contain	containing	1020:1029	arg1	domain					1013:1018	(NOD)-like receptor family pyrin domain	980:1018	(NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation	980:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	1	36	theme	Murray	215:220	arg1	LRE					239:241	LRE	239:241	LRE	239:241	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	36	theme	Murray	215:220	arg1	extract					230:236	Lycium ruthenicum Murray ethanol extract	197:236	Lycium ruthenicum Murray ethanol extract (LRE)	197:242	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	0	37	theme	experimental	130:141	arg1	colitis					143:149	dextran sulfate sodium-induced murine experimental colitis	92:149	dextran sulfate sodium-induced murine experimental colitis	92:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	1	38	theme	sulfate	370:376	arg1	DSS					386:388	DSS	386:388	DSS	386:388	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	38	theme	sulfate	370:376	arg1	sodium					378:383	a dextran sulfate sodium	360:383	a dextran sulfate sodium (DSS) induced experimental colitis mice model	360:429	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	39	theme	ethanol	222:228	arg1	LRE					239:241	LRE	239:241	LRE	239:241	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	39	theme	ethanol	222:228	arg1	extract					230:236	Lycium ruthenicum Murray ethanol extract	197:236	Lycium ruthenicum Murray ethanol extract (LRE)	197:242	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	0	40	theme	Murray	36:41	arg1	extract					51:57	Lycium ruthenicum Murray ethanol extract	18:57	Lycium ruthenicum Murray ethanol extract	18:57	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	1	41	theme	LRE	303:305	arg1	effect					293:298	the protective effect	278:298	the protective effect of LRE on inflammatory bowel disease (IBD)	278:341	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	2	42	theme	phenols/phenolic	520:535	arg1	acids					537:541	phenols/phenolic acids	520:541	phenols/phenolic acids	520:541	The results showed that a total of 129 compounds were tentatively identified, including phenols/phenolic acids, flavonoids and others.					
32285052	0	43	from	inflammation	76:87	arg1	colitis					143:149	dextran sulfate sodium-induced murine experimental colitis	92:149	dextran sulfate sodium-induced murine experimental colitis	92:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	4	44	theme	inflammasome	1041:1052	arg1	activation					1054:1063	3 (NLRP3) inflammasome activation	1031:1063	3 (NLRP3) inflammasome activation	1031:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	1	45	theme	extract	230:236	arg1	compositions					181:192	phytochemical compositions	167:192	phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE)	167:242	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	0	46	theme	ruthenicum	25:34	arg1	extract					51:57	Lycium ruthenicum Murray ethanol extract	18:57	Lycium ruthenicum Murray ethanol extract	18:57	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	4	47	theme	pyrin	1007:1011	arg1	domain					1013:1018	(NOD)-like receptor family pyrin domain	980:1018	(NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation	980:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	48	theme	kinase	837:842	arg1	pathways					851:858	mitogen-activated protein kinase (MAPK) pathways	811:858	mitogen-activated protein kinase (MAPK) pathways	811:858	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	0	49	theme	extract	51:57	arg1	intake					8:13	Dietary intake	0:13	Dietary intake of Lycium ruthenicum Murray ethanol extract	0:57	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	1	50	theme	inflammatory	310:321	arg1	IBD					338:340	IBD	338:340	IBD	338:340	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	50	theme	inflammatory	310:321	arg1	disease					329:335	inflammatory bowel disease	310:335	inflammatory bowel disease (IBD)	310:341	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	4	51	theme	cell	921:924	arg1	infiltration					926:937	immune cell infiltration	914:937	immune cell infiltration	914:937	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	3	52	theme	disease	639:645	arg1	increase					662:669	disease activity index increase	639:669	disease activity index increase	639:669	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	3	53	theme	LRE	567:569	arg1	supplementation					571:585	LRE supplementation	567:585	LRE supplementation	567:585	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	0	54	theme	ethanol	43:49	arg1	extract					51:57	Lycium ruthenicum Murray ethanol extract	18:57	Lycium ruthenicum Murray ethanol extract	18:57	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	1	55	theme	bowel	323:327	arg1	IBD					338:340	IBD	338:340	IBD	338:340	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	1	55	theme	bowel	323:327	arg1	disease					329:335	inflammatory bowel disease	310:335	inflammatory bowel disease (IBD)	310:341	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	4	56	theme	immune	914:919	arg1	infiltration					926:937	immune cell infiltration	914:937	immune cell infiltration	914:937	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	3	57	theme	colon	672:676	arg1	shortening					685:694	colon length shortening	672:694	colon length shortening	672:694	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	6	58	theme	dietary	1241:1247	arg1	strategy					1249:1256	a new dietary strategy	1235:1256	a new dietary strategy for preventing and ameliorating IBD as a functional food	1235:1313	Together, LRE supplementation might provide a new dietary strategy for preventing and ameliorating IBD as a functional food.					
32285052	4	59	theme	mitogen-activated	811:827	arg1	MAPK					845:848	MAPK	845:848	MAPK	845:848	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	4	59	theme	mitogen-activated	811:827	arg1	kinase					837:842	mitogen-activated protein kinase	811:842	mitogen-activated protein kinase (MAPK) pathways	811:858	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	3	60	theme	length	678:683	arg1	shortening					685:694	colon length shortening	672:694	colon length shortening	672:694	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	3	61	theme	weight	626:631	arg1	loss					633:636	DSS-induced body weight loss	609:636	DSS-induced body weight loss	609:636	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	1	62	theme	experimental	399:410	arg1	model					425:429	experimental colitis mice model	399:429	experimental colitis mice model	399:429	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	0	63	theme	colonic	68:74	arg1	inflammation					76:87	colonic inflammation	68:87	colonic inflammation in dextran sulfate sodium-induced murine experimental colitis	68:149	Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis.					
32285052	1	64	theme	colitis	412:418	arg1	model					425:429	experimental colitis mice model	399:429	experimental colitis mice model	399:429	In this study, phytochemical compositions of Lycium ruthenicum Murray ethanol extract (LRE) were analyzed by LC-ESI-MS/MS and the protective effect of LRE on inflammatory bowel disease (IBD) was evaluated in a dextran sulfate sodium (DSS) induced experimental colitis mice model.					
32285052	3	65	theme	DSS-induced	609:619	arg1	loss					633:636	DSS-induced body weight loss	609:636	DSS-induced body weight loss	609:636	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	4	66	theme	-like	985:989	arg1	domain					1013:1018	(NOD)-like receptor family pyrin domain	980:1018	(NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation	980:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32285052	3	67	theme	colonic	700:706	arg1	damage					721:726	colonic pathological damage	700:726	colonic pathological damage	700:726	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	3	68	theme	body	621:624	arg1	loss					633:636	DSS-induced body weight loss	609:636	DSS-induced body weight loss	609:636	LRE supplementation significantly reduced DSS-induced body weight loss, disease activity index increase, colon length shortening and colonic pathological damage.					
32285052	4	69	theme	NLRP3	1034:1038	arg1	activation					1054:1063	3 (NLRP3) inflammasome activation	1031:1063	3 (NLRP3) inflammasome activation	1031:1063	LRE inhibited intestinal inflammation by regulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, restored intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and suppressed (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation.					
32064408	1	0	theme	huge	169:172	arg1	variability					174:184	a huge variability	167:184	a huge variability in reducing sugars	167:203	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	6	1	theme	glycan	1299:1304	arg1	beneficial					1310:1319	beneficial	1310:1319	beneficial	1310:1319	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	6	1	theme	glycan	1299:1304	arg1	diversification					1265:1279	the diversification	1261:1279	the diversification of macromolecular glycan	1261:1304	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	1	2	theme	hydrolyzed	260:269	arg1	products					271:278	the hydrolyzed products	256:278	the hydrolyzed products of konjac flour with different concentrations	256:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	3	3	theme	solution	701:708	arg1	U/mL					681:684	20 U/mL	678:684	20 U/mL of β-mannanase solution	678:708	Hydrolysate of konjac flour, using concentrations ranging from 50 to 200 g/L, was directly added into 20 U/mL of β-mannanase solution.					
32064408	4	4	from	solution	783:790	arg1	proportion					744:753	the proportion	740:753	the proportion of the water content in the solution	740:790	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	7	5	with	proportion	1393:1402	arg1	content					1439:1445	free water content	1428:1445	free water content	1428:1445	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	2	6	theme	hydrolysis	558:567	arg1	model					569:573	the high-concentration solute hydrolysis model	528:573	the high-concentration solute hydrolysis model	528:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	2	7	from	preparation	446:456	arg1	flour					490:494	konjac flour	483:494	konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model	483:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	1	8	from	variability	174:184	arg1	products					271:278	the hydrolyzed products	256:278	the hydrolyzed products of konjac flour with different concentrations	256:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	5	9	theme	mPa·s	965:969	arg1	range					950:954	the range	946:954	the range of 30-20 mPa·s	946:969	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	0	10	theme	Enzymatic	101:109	arg1	Hydrolysis					111:120	Enzymatic Hydrolysis	101:120	Enzymatic Hydrolysis of High-Concentration Konjac Flour	101:155	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	6	11	theme	sugars	1249:1254	arg1	production					1226:1235	the production	1222:1235	the production of reducing sugars	1222:1254	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	4	12	theme	content	768:774	arg1	proportion					744:753	the proportion	740:753	the proportion of the water content in the solution	740:790	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	1	13	theme	konjac	283:288	arg1	flour					290:294	konjac flour	283:294	konjac flour with different concentrations	283:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	0	14	theme	High-Concentration	125:142	arg1	Flour					151:155	High-Concentration Konjac Flour	125:155	High-Concentration Konjac Flour	125:155	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	6	15	theme	reducing	1240:1247	arg1	sugars					1249:1254	reducing sugars	1240:1254	reducing sugars	1240:1254	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	4	16	from	content	768:774	arg1	solution					783:790	the solution	779:790	the solution	779:790	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	2	17	theme	oligosaccharides	461:476	arg1	preparation					446:456	the preparation	442:456	the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model	442:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	2	18	from	flour	490:494	arg1	preparation					446:456	the preparation	442:456	the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model	442:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	2	18	from	flour	490:494	arg1	oligosaccharides					461:476	oligosaccharides	461:476	oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model	461:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	7	19	theme	reducing	1407:1414	arg1	sugars					1416:1421	reducing sugars	1407:1421	reducing sugars	1407:1421	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	2	20	theme	high-concentration	532:549	arg1	model					569:573	the high-concentration solute hydrolysis model	528:573	the high-concentration solute hydrolysis model	528:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	0	21	theme	Article	9:15	arg1	Analysis					37:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	7	22	theme	water	1433:1437	arg1	content					1439:1445	free water content	1428:1445	free water content	1428:1445	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	4	23	theme	polysaccharides	847:861	arg1	viscosity					807:815	the viscosity	803:815	the viscosity of the solution	803:831	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	4	23	theme	polysaccharides	847:861	arg1	DP					841:842	the DP	837:842	the DP of polysaccharides	837:861	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	7	24	theme	product	1557:1563	arg1	diversity					1565:1573	product diversity	1557:1573	product diversity	1557:1573	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	2	25	theme	average	397:403	arg1	degree					405:410	the average degree	393:410	the average degree of polymerization (DP)	393:433	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	0	26	theme	Research	0:7	arg1	Analysis					37:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	1	27	theme	flour	290:294	arg1	products					271:278	the hydrolyzed products	256:278	the hydrolyzed products of konjac flour with different concentrations	256:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	1	28	with	flour	290:294	arg1	concentrations					311:324	different concentrations	301:324	different concentrations	301:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	7	29	theme	free	1428:1431	arg1	content					1439:1445	free water content	1428:1445	free water content	1428:1445	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	0	30	theme	Composition	25:35	arg1	Analysis					37:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	0	31	theme	Flour	151:155	arg1	Hydrolysis					111:120	Enzymatic Hydrolysis	101:120	Enzymatic Hydrolysis of High-Concentration Konjac Flour	101:155	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	4	32	from	proportion	744:753	arg1	solution					783:790	the solution	779:790	the solution	779:790	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	3	33	theme	konjac	591:596	arg1	flour					598:602	konjac flour	591:602	konjac flour	591:602	Hydrolysate of konjac flour, using concentrations ranging from 50 to 200 g/L, was directly added into 20 U/mL of β-mannanase solution.					
32064408	0	34	theme	Product	17:23	arg1	Analysis					37:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	1	35	theme	different	301:309	arg1	concentrations					311:324	different concentrations	301:324	different concentrations	301:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	0	36	theme	Konjac	144:149	arg1	Flour					151:155	High-Concentration Konjac Flour	125:155	High-Concentration Konjac Flour	125:155	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	7	37	theme	solid-state	1528:1538	arg1	method					1540:1545	the high-concentration solid-state method	1505:1545	the high-concentration solid-state method	1505:1545	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	3	38	theme	flour	598:602	arg1	Hydrolysate					576:586	Hydrolysate	576:586	Hydrolysate	576:586	Hydrolysate of konjac flour, using concentrations ranging from 50 to 200 g/L, was directly added into 20 U/mL of β-mannanase solution.					
32064408	3	39	dep	200	645:647	arg1	to					642:643	to	642:643	to	642:643	Hydrolysate of konjac flour, using concentrations ranging from 50 to 200 g/L, was directly added into 20 U/mL of β-mannanase solution.					
32064408	2	40	theme	konjac	483:488	arg1	flour					490:494	konjac flour	483:494	konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model	483:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	1	41	from	products	271:278	arg1	composition					221:231	composition	221:231	composition	221:231	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	1	41	from	products	271:278	arg1	viscosity					206:214	viscosity	206:214	viscosity	206:214	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	1	41	from	products	271:278	arg1	variability					174:184	a huge variability	167:184	a huge variability in reducing sugars	167:203	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	5	42	theme	average	1061:1067	arg1	DP					1069:1070	the average DP	1057:1070	the average DP of the polysaccharides	1057:1093	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	2	43	theme	polymerization	415:428	arg1	degree					405:410	the average degree	393:410	the average degree of polymerization (DP)	393:433	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	2	43	theme	polymerization	415:428	arg1	viscosity					378:386	viscosity	378:386	viscosity	378:386	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	2	43	theme	polymerization	415:428	arg1	sugars					370:375	reducing sugars	361:375	reducing sugars	361:375	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	5	44	theme	2.42-9.78	1126:1134	arg1	range					1117:1121	the range	1113:1121	the range of 2.42-9.78	1113:1134	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	6	45	theme	sugars	1354:1359	arg1	preparation					1328:1338	the preparation	1324:1338	the preparation of functional sugars	1324:1359	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	7	46	theme	high-concentration	1509:1526	arg1	method					1540:1545	the high-concentration solid-state method	1505:1545	the high-concentration solid-state method	1505:1545	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	1	47	from	composition	221:231	arg1	products					271:278	the hydrolyzed products	256:278	the hydrolyzed products of konjac flour with different concentrations	256:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	5	48	theme	hydrolysate	912:922	arg1	viscosity					895:903	the viscosity	891:903	the viscosity of the hydrolysate	891:922	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	0	49	theme	Process	50:56	arg1	Research					58:65	Process Research	50:65	Process Research	50:65	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	6	50	theme	macromolecular	1284:1297	arg1	glycan					1299:1304	macromolecular glycan	1284:1304	macromolecular glycan	1284:1304	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	7	51	theme	oligosaccharides	1484:1499	arg1	preparation					1469:1479	the preparation	1465:1479	the preparation of oligosaccharides via the high-concentration solid-state method	1465:1545	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	4	52	theme	solution	824:831	arg1	viscosity					807:815	the viscosity	803:815	the viscosity of the solution	803:831	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	4	52	theme	solution	824:831	arg1	DP					841:842	the DP	837:842	the DP of polysaccharides	837:861	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	1	53	from	viscosity	206:214	arg1	products					271:278	the hydrolyzed products	256:278	the hydrolyzed products of konjac flour with different concentrations	256:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	2	54	theme	reducing	361:368	arg1	sugars					370:375	reducing sugars	361:375	reducing sugars	361:375	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32064408	6	55	theme	high	1158:1161	arg1	beneficial					1196:1205	beneficial	1196:1205	beneficial	1196:1205	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	6	55	theme	high	1158:1161	arg1	concentration					1163:1175	a high concentration	1156:1175	a high concentration of hydrolysate	1156:1190	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	3	56	theme	β-mannanase	689:699	arg1	solution					701:708	β-mannanase solution	689:708	β-mannanase solution	689:708	Hydrolysate of konjac flour, using concentrations ranging from 50 to 200 g/L, was directly added into 20 U/mL of β-mannanase solution.					
32064408	6	57	theme	hydrolysate	1180:1190	arg1	beneficial					1196:1205	beneficial	1196:1205	beneficial	1196:1205	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	6	57	theme	hydrolysate	1180:1190	arg1	concentration					1163:1175	a high concentration	1156:1175	a high concentration of hydrolysate	1156:1190	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	4	58	theme	water	762:766	arg1	content					768:774	the water content	758:774	the water content in the solution	758:790	The results showed that when the proportion of the water content in the solution decreased, the viscosity of the solution and the DP of polysaccharides changed significantly.					
32064408	1	59	from	oligosaccharides	236:251	arg1	products					271:278	the hydrolyzed products	256:278	the hydrolyzed products of konjac flour with different concentrations	256:324	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	7	60	theme	sugars	1416:1421	arg1	proportion					1393:1402	the proportion	1389:1402	the proportion of reducing sugars with free water content	1389:1445	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	7	60	theme	sugars	1416:1421	arg1	high					1451:1454	high	1451:1454	high	1451:1454	Moreover, we observed that the proportion of reducing sugars with free water content was high and that the preparation of oligosaccharides via the high-concentration solid-state method increased product diversity.					
32064408	6	61	theme	functional	1343:1352	arg1	sugars					1354:1359	functional sugars	1343:1359	functional sugars	1343:1359	We also found that a high concentration of hydrolysate was beneficial for decreasing the production of reducing sugars, and the diversification of macromolecular glycan was beneficial to the preparation of functional sugars.					
32064408	1	62	theme	oligosaccharides	236:251	arg1	composition					221:231	composition	221:231	composition	221:231	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	1	62	theme	oligosaccharides	236:251	arg1	viscosity					206:214	viscosity	206:214	viscosity	206:214	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	1	62	theme	oligosaccharides	236:251	arg1	variability					174:184	a huge variability	167:184	a huge variability in reducing sugars	167:203	There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations.					
32064408	0	63	theme	Oligosaccharides	70:85	arg1	Analysis					37:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis	0:44	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	0	63	theme	Oligosaccharides	70:85	arg1	Research					58:65	Process Research	50:65	Process Research	50:65	Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.					
32064408	5	64	theme	g/L	1049:1051	arg1	range					1035:1039	the range	1031:1039	the range of 9-13 g/L	1031:1051	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	5	65	theme	reducing	997:1004	arg1	sugars					1006:1011	the reducing sugars	993:1011	the reducing sugars	993:1011	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	5	66	theme	sugars	1006:1011	arg1	concentration					976:988	the concentration	972:988	the concentration of the reducing sugars	972:1011	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	5	67	theme	polysaccharides	1079:1093	arg1	DP					1069:1070	the average DP	1057:1070	the average DP of the polysaccharides	1057:1093	When the viscosity of the hydrolysate was controlled within the range of 30-20 mPa·s, the concentration of the reducing sugars was maintained in the range of 9-13 g/L and the average DP of the polysaccharides was controlled in the range of 2.42-9.78.					
32064408	2	68	theme	solute	551:556	arg1	model					569:573	the high-concentration solute hydrolysis model	528:573	the high-concentration solute hydrolysis model	528:573	We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model.					
32942699	8	0	theme	emulsifier-containing	1315:1335	arg1	foods					1337:1341	emulsifier-containing foods	1315:1341	emulsifier-containing foods	1315:1341	The frequency of consuming emulsifier-containing foods decreased by 94.6% (SD 10.3%).					
32942699	11	1	theme	dietary	1740:1746	arg1	restriction					1748:1758	dietary restriction	1740:1758	dietary restriction of emulsifiers	1740:1773	A range of emulsifiers may influence intestinal inflammation in mice, and dietary restriction of emulsifiers is feasible.					
32942699	2	2	from	feasibility	353:363	arg1	markers					297:303	markers	297:303	markers of intestinal inflammation in mice	297:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	3	theme	intestinal	308:317	arg1	inflammation					319:330	intestinal inflammation	308:330	intestinal inflammation in mice	308:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	4	from	effect	251:256	arg1	markers					297:303	markers	297:303	markers of intestinal inflammation in mice	297:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	5	theme	emulsifier	425:434	arg1	classes					436:442	all emulsifier classes	421:442	all emulsifier classes in Crohn's disease	421:461	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	10	6	theme	control	1594:1600	arg1	scores					1602:1607	disease control scores	1586:1607	disease control scores	1586:1607	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	1	7	theme	food	145:148	arg1	emulsifiers					159:169	food additive emulsifiers	145:169	food additive emulsifiers	145:169	There is an association between food additive emulsifiers and the prevalence of Crohn's disease.					
32942699	3	8	dep	emulsifiers	506:516	arg1	polysorbate-80					540:553	polysorbate-80	540:553	polysorbate-80	540:553	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	3	8	dep	emulsifiers	506:516	arg1	lecithin					560:567	soy lecithin	556:567	soy lecithin	556:567	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	3	8	dep	emulsifiers	506:516	arg1	carboxymethycellose					519:537	carboxymethycellose	519:537	carboxymethycellose	519:537	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	3	8	dep	emulsifiers	506:516	arg1	gum					570:572	gum arabic	570:579	gum arabic	570:579	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	8	9	dep	decreased	1343:1351	arg1	%					1370:1370	SD 10.3%	1363:1370	SD 10.3%	1363:1370	The frequency of consuming emulsifier-containing foods decreased by 94.6% (SD 10.3%).					
32942699	10	10	theme	disease	1586:1592	arg1	scores					1602:1607	disease control scores	1586:1607	disease control scores	1586:1607	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	9	11	theme	low	1446:1448	arg1	diet					1461:1464	habitual (median 81.5 (IQR 25.0)) and low emulsifier diet	1408:1464	diet	1461:1464	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	12	12	theme	low	1827:1829	arg1	diet					1842:1845	a low emulsifier diet	1825:1845	a low emulsifier diet	1825:1845	Trials investigating the efficacy of a low emulsifier diet in Crohn's disease are warranted.					
32942699	10	13	dep	=	1572:1572	arg1	1.4					1559:1561	1.4	1559:1561	1.4	1559:1561	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	13	dep	=	1572:1572	arg1	3.9					1564:1566	3.9	1564:1566	3.9	1564:1566	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	13	dep	=	1572:1572	arg1	IQR					1546:1548	IQR 5.3	1546:1552	IQR 5.3	1546:1552	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	13	dep	=	1572:1572	arg1	3.0					1541:1543	3.0	1541:1543	3.0	1541:1543	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	3	14	theme	emulsifiers	506:516	arg1	classes					495:501	different classes	485:501	different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic)	485:580	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	3	15	theme	metabolism	654:663	arg1	markers					626:632	markers	626:632	markers of inflammation and metabolism	626:663	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	4	16	theme	emulsifier	686:695	arg1	diet					697:700	A low emulsifier diet	680:700	A low emulsifier diet	680:700	A low emulsifier diet was developed to restrict all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease.					
32942699	2	17	from	markers	297:303	arg1	mice					335:338	mice	335:338	mice	335:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	3	18	theme	different	485:493	arg1	classes					495:501	different classes	485:501	different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic)	485:580	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	6	19	theme	murine	1029:1034	arg1	length					1044:1049	lower murine colonic length	1023:1049	lower murine colonic length	1023:1049	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	9	20	dep	=	1482:1482	arg1	24.0					1473:1476	24.0	1473:1476	24.0	1473:1476	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	2	21	from	impact	399:404	arg1	markers					297:303	markers	297:303	markers of intestinal inflammation in mice	297:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	10	22	dep	reduced	1525:1531	arg1	=					1572:1572	=	1572:1572	=	1572:1572	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	6	23	dep	polysorbate-80	1138:1151	arg1	=					1171:1171	=	1171:1171	=	1171:1171	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	1	24	theme	additive	150:157	arg1	emulsifiers					159:169	food additive emulsifiers	145:169	food additive emulsifiers	145:169	There is an association between food additive emulsifiers and the prevalence of Crohn's disease.					
32942699	9	25	dep	median	1418:1423	arg1	IQR					1431:1433	IQR 25.0	1431:1438	IQR 25.0	1431:1438	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	3	26	theme	inflammation	637:648	arg1	markers					626:632	markers	626:632	markers of inflammation and metabolism	626:663	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	4	27	theme	feasibility	763:773	arg1	classes					732:738	all classes	728:738	all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease	728:842	A low emulsifier diet was developed to restrict all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease.					
32942699	11	28	theme	emulsifiers	1763:1773	arg1	restriction					1748:1758	dietary restriction	1740:1758	dietary restriction of emulsifiers	1740:1773	A range of emulsifiers may influence intestinal inflammation in mice, and dietary restriction of emulsifiers is feasible.					
32942699	2	29	theme	symptom	391:397	arg1	impact					399:404	symptom impact	391:404	symptom impact	391:404	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	7	30	theme	feasibility	1269:1279	arg1	study					1281:1285	the feasibility study	1265:1285	the feasibility study	1265:1285	All 20 participants completed the feasibility study.					
32942699	0	31	theme	Impact	12:17	arg1	Length					27:32	Emulsifiers Impact Colonic Length	0:32	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction	0:67	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	6	32	theme	mean	1074:1077	arg1	cm					1083:1084	mean 9.5 cm	1074:1084	mean 9.5 cm	1074:1084	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	2	33	theme	classes	271:277	arg1	feasibility					353:363	the feasibility	349:363	the feasibility	349:363	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	33	theme	classes	271:277	arg1	impact					399:404	symptom impact	391:404	symptom impact	391:404	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	33	theme	classes	271:277	arg1	effect					251:256	the effect	247:256	: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice	241:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	33	theme	classes	271:277	arg1	adequacy					378:385	nutritional adequacy	366:385	nutritional adequacy	366:385	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	4	34	theme	stable	821:826	arg1	disease					836:842	stable Crohn's disease	821:842	stable Crohn's disease	821:842	A low emulsifier diet was developed to restrict all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease.					
32942699	9	35	dep	habitual	1408:1415	arg1	median					1418:1423	median 81.5	1418:1428	median 81.5	1418:1428	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	9	35	dep	habitual	1408:1415	arg1	=					1482:1482	=	1482:1482	=	1482:1482	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	0	36	theme	Emulsifiers	0:10	arg1	Length					27:32	Emulsifiers Impact Colonic Length	0:32	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction	0:67	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	10	37	dep	=	1656:1656	arg1	13.5					1619:1622	13.5	1619:1622	13.5	1619:1622	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	37	dep	=	1656:1656	arg1	15.5					1638:1641	15.5	1638:1641	15.5	1638:1641	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	37	dep	=	1656:1656	arg1	IQR					1625:1627	IQR 6.0	1625:1631	IQR 6.0	1625:1631	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	37	dep	=	1656:1656	arg1	IQR					1644:1646	IQR 3.0	1644:1650	IQR 3.0	1644:1650	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	2	38	theme	different	261:269	arg1	classes					271:277	different classes	261:277	different classes of emulsifiers	261:292	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	6	39	dep	=	1171:1171	arg1	cm					1158:1159	8.2 cm	1154:1159	8.2 cm (0.34)	1154:1166	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	6	39	dep	=	1171:1171	arg1	0.34					1162:1165	0.34	1162:1165	0.34	1162:1165	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	5	40	theme	nutrient	925:932	arg1	intake					934:939	nutrient intake	925:939	nutrient intake	925:939	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	0	41	with	People	84:89	arg1	Disease					104:110	Crohn's Disease	96:110	Crohn's Disease	96:110	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	4	42	theme	low	682:684	arg1	diet					697:700	A low emulsifier diet	680:700	A low emulsifier diet	680:700	A low emulsifier diet was developed to restrict all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease.					
32942699	6	43	dep	resulted	1011:1018	arg1	compared					1051:1058	compared	1051:1058	compared with control (mean 9.5 cm (SEM 0.20))	1051:1096	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	6	44	dep	cm	1083:1084	arg1	SEM					1087:1089	SEM 0.20	1087:1094	SEM 0.20	1087:1094	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	2	45	theme	nutritional	366:376	arg1	adequacy					378:385	nutritional adequacy	366:385	nutritional adequacy	366:385	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	12	46	theme	diet	1842:1845	arg1	efficacy					1813:1820	the efficacy	1809:1820	the efficacy of a low emulsifier diet in Crohn's disease	1809:1864	Trials investigating the efficacy of a low emulsifier diet in Crohn's disease are warranted.					
32942699	6	47	dep	=	1225:1225	arg1	cm					1212:1213	8.0 cm	1208:1213	8.0 cm (0.35)	1208:1220	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	6	47	dep	=	1225:1225	arg1	0.35					1216:1219	0.35	1216:1219	0.35	1216:1219	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	2	48	from	classes	436:442	arg1	disease					455:461	Crohn's disease	447:461	Crohn's disease	447:461	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	10	49	theme	reduced	1525:1531	arg1	symptoms					1516:1523	Crohn's disease-related symptoms	1492:1523	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006)	1492:1579	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	10	50	theme	disease-related	1500:1514	arg1	symptoms					1516:1523	Crohn's disease-related symptoms	1492:1523	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006)	1492:1579	Crohn's disease-related symptoms reduced (median 3.0 (IQR 5.3) vs. 1.4 (3.9), p = 0.006), and disease control scores improved (13.5 (IQR 6.0) vs. 15.5 (IQR 3.0), p = 0.026).					
32942699	9	51	theme	Food-related	1374:1385	arg1	QoL					1387:1389	Food-related QoL	1374:1389	Food-related QoL	1374:1389	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	12	52	theme	emulsifier	1831:1840	arg1	diet					1842:1845	a low emulsifier diet	1825:1845	a low emulsifier diet	1825:1845	Trials investigating the efficacy of a low emulsifier diet in Crohn's disease are warranted.					
32942699	0	53	from	Feasible	72:79	arg1	People					84:89	People	84:89	People with Crohn's Disease	84:110	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	4	54	theme	emulsifiers	743:753	arg1	classes					732:738	all classes	728:738	all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease	728:842	A low emulsifier diet was developed to restrict all classes of emulsifiers and its feasibility measured over 14-days in 20 participants with stable Crohn's disease.					
32942699	6	55	dep	control	1065:1071	arg1	cm					1083:1084	mean 9.5 cm	1074:1084	mean 9.5 cm	1074:1084	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	11	56	theme	emulsifiers	1677:1687	arg1	range					1668:1672	A range	1666:1672	A range of emulsifiers	1666:1687	A range of emulsifiers may influence intestinal inflammation in mice, and dietary restriction of emulsifiers is feasible.					
32942699	3	57	theme	arabic	574:579	arg1	carboxymethycellose					519:537	carboxymethycellose	519:537	carboxymethycellose	519:537	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	3	57	theme	arabic	574:579	arg1	gum					570:572	gum arabic	570:579	gum arabic	570:579	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	5	58	theme	food-related	945:956	arg1	QoL					975:977	QoL	975:977	QoL	975:977	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	58	theme	food-related	945:956	arg1	quality					958:964	food-related quality	945:964	food-related quality of life (QoL)	945:978	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	0	59	theme	Emulsifier	46:55	arg1	Restriction					57:67	Emulsifier Restriction	46:67	Emulsifier Restriction	46:67	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	5	60	theme	body	896:899	arg1	symptoms					869:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	60	theme	body	896:899	arg1	weight					901:906	body weight	896:906	body weight	896:906	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	2	61	from	mice	335:338	arg1	markers					297:303	markers	297:303	markers of intestinal inflammation in mice	297:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	8	62	theme	SD	1363:1364	arg1	%					1370:1370	SD 10.3%	1363:1370	SD 10.3%	1363:1370	The frequency of consuming emulsifier-containing foods decreased by 94.6% (SD 10.3%).					
32942699	2	63	from	adequacy	378:385	arg1	markers					297:303	markers	297:303	markers of intestinal inflammation in mice	297:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	5	64	theme	life	969:972	arg1	intake					934:939	nutrient intake	925:939	nutrient intake	925:939	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	64	theme	life	969:972	arg1	QoL					975:977	QoL	975:977	QoL	975:977	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	64	theme	life	969:972	arg1	weight					901:906	body weight	896:906	body weight	896:906	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	64	theme	life	969:972	arg1	symptoms					869:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	64	theme	life	969:972	arg1	quality					958:964	food-related quality	945:964	food-related quality of life (QoL)	945:978	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	64	theme	life	969:972	arg1	composition					912:922	composition	912:922	composition	912:922	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	64	theme	life	969:972	arg1	control					887:893	disease control	879:893	disease control	879:893	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	2	65	dep	feasibility	353:363	arg1	ii					345:346	ii	345:346	ii	345:346	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	9	66	theme	emulsifier	1450:1459	arg1	diet					1461:1464	habitual (median 81.5 (IQR 25.0)) and low emulsifier diet	1408:1464	diet	1461:1464	Food-related QoL improved between habitual (median 81.5 (IQR 25.0)) and low emulsifier diet (90.0 (24.0), p = 0.028).					
32942699	2	67	dep	effect	251:256	arg1	i					244:244	i	244:244	i	244:244	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	2	68	from	inflammation	319:330	arg1	mice					335:338	mice	335:338	mice	335:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	11	69	theme	intestinal	1703:1712	arg1	inflammation					1714:1725	intestinal inflammation	1703:1725	intestinal inflammation in mice	1703:1733	A range of emulsifiers may influence intestinal inflammation in mice, and dietary restriction of emulsifiers is feasible.					
32942699	2	70	theme	emulsifiers	282:292	arg1	classes					271:277	different classes	261:277	different classes of emulsifiers	261:292	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	5	71	theme	disease	879:885	arg1	symptoms					869:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	71	theme	disease	879:885	arg1	control					887:893	disease control	879:893	disease control	879:893	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	1	72	theme	disease	201:207	arg1	emulsifiers					159:169	food additive emulsifiers	145:169	food additive emulsifiers	145:169	There is an association between food additive emulsifiers and the prevalence of Crohn's disease.					
32942699	1	72	theme	disease	201:207	arg1	prevalence					179:188	the prevalence	175:188	the prevalence of Crohn's disease	175:207	There is an association between food additive emulsifiers and the prevalence of Crohn's disease.					
32942699	5	73	theme	disease-related	853:867	arg1	symptoms					869:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms	845:876	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	73	theme	disease-related	853:867	arg1	control					887:893	disease control	879:893	disease control	879:893	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	73	theme	disease-related	853:867	arg1	weight					901:906	body weight	896:906	body weight	896:906	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	5	73	theme	disease-related	853:867	arg1	composition					912:922	composition	912:922	composition	912:922	Crohn's disease-related symptoms, disease control, body weight and composition, nutrient intake and food-related quality of life (QoL) were measured.					
32942699	6	74	theme	colonic	1036:1042	arg1	length					1044:1049	lower murine colonic length	1023:1049	lower murine colonic length	1023:1049	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	0	75	from	People	84:89	arg1	Feasible					72:79	Feasible	72:79	Feasible	72:79	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	3	76	theme	soy	556:558	arg1	lecithin					560:567	soy lecithin	556:567	soy lecithin	556:567	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	3	76	theme	soy	556:558	arg1	carboxymethycellose					519:537	carboxymethycellose	519:537	carboxymethycellose	519:537	Mice were exposed to different classes of emulsifiers (carboxymethycellose, polysorbate-80, soy lecithin, gum arabic) in drinking water for 12-weeks, after which markers of inflammation and metabolism were measured.					
32942699	6	77	theme	lower	1023:1027	arg1	length					1044:1049	lower murine colonic length	1023:1049	lower murine colonic length	1023:1049	All emulsifiers resulted in lower murine colonic length compared with control (mean 9.5 cm (SEM 0.20)), but this only reached significance for polysorbate-80 (8.2 cm (0.34), p = 0.024) and carboxymethylcellulose (8.0 cm (0.35), p = 0.013).					
32942699	12	78	from	efficacy	1813:1820	arg1	disease					1858:1864	Crohn's disease	1850:1864	Crohn's disease	1850:1864	Trials investigating the efficacy of a low emulsifier diet in Crohn's disease are warranted.					
32942699	0	79	from	Length	27:32	arg1	Mice					37:40	Mice	37:40	Mice	37:40	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	0	79	from	Length	27:32	arg1	Restriction					57:67	Emulsifier Restriction	46:67	Emulsifier Restriction	46:67	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	0	80	theme	Colonic	19:25	arg1	Length					27:32	Emulsifiers Impact Colonic Length	0:32	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction	0:67	Emulsifiers Impact Colonic Length in Mice and Emulsifier Restriction is Feasible in People with Crohn's Disease.					
32942699	2	81	theme	inflammation	319:330	arg1	markers					297:303	markers	297:303	markers of intestinal inflammation in mice	297:338	This study aimed to investigate: (i) the effect of different classes of emulsifiers on markers of intestinal inflammation in mice and (ii) the feasibility, nutritional adequacy and symptom impact of restricting all emulsifier classes in Crohn's disease.					
32942699	11	82	from	inflammation	1714:1725	arg1	mice					1730:1733	mice	1730:1733	mice	1730:1733	A range of emulsifiers may influence intestinal inflammation in mice, and dietary restriction of emulsifiers is feasible.					
32679323	8	0	theme	iron	1270:1273	arg1	oxide					1275:1279	hydroxyl iron oxide	1261:1279	hydroxyl iron oxide	1261:1279	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	4	1	theme	ions	770:773	arg1	concentrations					775:788	the increasing m/v and co-existing ions concentrations	735:788	the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+	735:813	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	1	2	theme	OH	155:156	arg1	oxide/chitosan					133:146	A goethite/graphene oxide/chitosan	113:146	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent	113:188	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	1	2	theme	OH	155:156	arg1	/GO/CS					158:163	α-FeO(OH)/GO/CS	149:163	α-FeO(OH)/GO/CS	149:163	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	9	3	theme	removal	1340:1346	arg1	high					1372:1375	high	1372:1375	high	1372:1375	After the fifth desorption, the removal efficiency was still as high as 79.6%, indicating excellent reusability.					
32679323	9	3	theme	removal	1340:1346	arg1	efficiency					1348:1357	the removal efficiency	1336:1357	the removal efficiency	1336:1357	After the fifth desorption, the removal efficiency was still as high as 79.6%, indicating excellent reusability.					
32679323	4	4	theme	Fe3+	810:813	arg1	concentrations					775:788	the increasing m/v and co-existing ions concentrations	735:788	the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+	735:813	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	4	5	theme	co-existing	758:768	arg1	ions					770:773	co-existing ions	758:773	co-existing ions	758:773	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	3	6	theme	contact	444:450	arg1	time					452:455	contact time	444:455	contact time	444:455	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	6	theme	contact	444:450	arg1	factors					403:409	several factors	395:409	several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature)	395:491	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	5	7	theme	wide	854:857	arg1	range					862:866	a wide pH range	852:866	a wide pH range of 3-10	852:874	As(III) adsorption remained high at a wide pH range of 3-10.					
32679323	7	8	theme	Sips	1043:1046	arg1	models					1048:1053	the Freundlich and Sips models	1024:1053	the Freundlich and Sips models	1024:1053	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	9	9	theme	excellent	1398:1406	arg1	reusability					1408:1418	excellent reusability	1398:1418	excellent reusability	1398:1418	After the fifth desorption, the removal efficiency was still as high as 79.6%, indicating excellent reusability.					
32679323	9	10	theme	fifth	1318:1322	arg1	desorption					1324:1333	the fifth desorption	1314:1333	the fifth desorption	1314:1333	After the fifth desorption, the removal efficiency was still as high as 79.6%, indicating excellent reusability.					
32679323	4	11	theme	initial	662:668	arg1	concentration					670:682	the increasing initial concentration	647:682	the increasing initial concentration	647:682	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	7	12	theme	maximum	1107:1113	arg1	289.42 mg/g					1139:1149	289.42 mg/g	1139:1149	289.42 mg/g	1139:1149	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	7	12	theme	maximum	1107:1113	arg1	capacity					1126:1133	the maximum adsorption capacity	1103:1133	the maximum adsorption capacity	1103:1133	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	7	13	theme	Freundlich	1028:1037	arg1	models					1048:1053	the Freundlich and Sips models	1024:1053	the Freundlich and Sips models	1024:1053	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	8	14	theme	major	1289:1293	arg1	contributor					1295:1305	the major contributor	1285:1305	the major contributor	1285:1305	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	8	14	theme	major	1289:1293	arg1	complexation					1223:1234	the complexation	1219:1234	the complexation between As(III) ions and hydroxyl iron oxide	1219:1279	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	4	15	theme	increasing	739:748	arg1	concentrations					775:788	the increasing m/v and co-existing ions concentrations	735:788	the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+	735:813	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	3	16	theme	co-existing	458:468	arg1	ions					470:473	co-existing ions	458:473	co-existing ions	458:473	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	7	17	theme	As	1000:1001	arg1	adsorption					1008:1017	As(III) adsorption	1000:1017	As(III) adsorption	1000:1017	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	4	18	theme	As	613:614	arg1	adsorption					621:630	As(III) adsorption	613:630	As(III) adsorption	613:630	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	7	19	with	fit	993:995	arg1	models					1048:1053	the Freundlich and Sips models	1024:1053	the Freundlich and Sips models	1024:1053	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	3	20	theme	several	395:401	arg1	time					452:455	contact time	444:455	contact time	444:455	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	20	theme	several	395:401	arg1	temperature					480:490	temperature	480:490	temperature	480:490	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	20	theme	several	395:401	arg1	pH					435:436	pH	435:436	pH	435:436	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	20	theme	several	395:401	arg1	m/v					439:441	m/v	439:441	m/v	439:441	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	20	theme	several	395:401	arg1	factors					403:409	several factors	395:409	several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature)	395:491	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	20	theme	several	395:401	arg1	concentration					420:432	initial concentration	412:432	initial concentration	412:432	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	0	21	theme	efficient	7:15	arg1	removal					17:23	Highly efficient removal	0:23	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene	0:81	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene oxide/chitosan nanocomposite.					
32679323	10	22	theme	aqueous	1488:1494	arg1	solutions					1496:1504	aqueous solutions	1488:1504	aqueous solutions	1488:1504	Thus, this composite had great potential for removing As(III) from aqueous solutions.					
32679323	10	23	attach	removing	1466:1473	arg1	solutions					1496:1504	aqueous solutions	1488:1504	aqueous solutions	1488:1504	Thus, this composite had great potential for removing As(III) from aqueous solutions.					
32679323	10	23	attach	removing	1466:1473	arg2	potential					1452:1460	great potential	1446:1460	great potential for removing As(III) from aqueous solutions	1446:1504	Thus, this composite had great potential for removing As(III) from aqueous solutions.					
32679323	6	24	theme	kinetic	933:939	arg1	model					941:945	a pseudo-second-order kinetic model	911:945	a pseudo-second-order kinetic model	911:945	The adsorption was well fitted to a pseudo-second-order kinetic model and was endothermic and spontaneous.					
32679323	1	25	theme	aqueous	243:249	arg1	solution					251:258	aqueous solution	243:258	aqueous solution	243:258	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	4	26	theme	m/v	750:752	arg1	concentrations					775:788	the increasing m/v and co-existing ions concentrations	735:788	the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+	735:813	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	10	27	theme	great	1446:1450	arg1	potential					1452:1460	great potential	1446:1460	great potential for removing As(III) from aqueous solutions	1446:1504	Thus, this composite had great potential for removing As(III) from aqueous solutions.					
32679323	3	28	theme	As	496:497	arg1	adsorption					504:513	As(III) adsorption	496:513	As(III) adsorption	496:513	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	1	29	theme	adsorbent	180:188	arg1	oxide/chitosan					133:146	A goethite/graphene oxide/chitosan	113:146	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent	113:188	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	1	29	theme	adsorbent	180:188	arg1	/GO/CS					158:163	α-FeO(OH)/GO/CS	149:163	α-FeO(OH)/GO/CS	149:163	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	8	30	theme	hydroxyl	1261:1268	arg1	oxide					1275:1279	hydroxyl iron oxide	1261:1279	hydroxyl iron oxide	1261:1279	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	7	31	theme	best	988:991	arg1	fit					993:995	The best fit	984:995	The best fit of As(III) adsorption with the Freundlich and Sips models	984:1053	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	4	32	theme	increasing	651:660	arg1	concentration					670:682	the increasing initial concentration	647:682	the increasing initial concentration	647:682	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	3	33	theme	initial	412:418	arg1	factors					403:409	several factors	395:409	several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature)	395:491	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	33	theme	initial	412:418	arg1	concentration					420:432	initial concentration	412:432	initial concentration	412:432	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	7	34	theme	adsorption	1008:1017	arg1	fit					993:995	The best fit	984:995	The best fit of As(III) adsorption with the Freundlich and Sips models	984:1053	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	4	35	theme	contact	685:691	arg1	time					693:696	contact time	685:696	contact time	685:696	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	3	36	theme	equilibrium	551:561	arg1	isotherm					563:570	equilibrium isotherm	551:570	equilibrium isotherm	551:570	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	2	37	theme	XRD	307:309	arg1	techniques					320:329	FTIR, XPS, XRD, and EDS techniques	296:329	FTIR, XPS, XRD, and EDS techniques	296:329	The composite was characterized by FTIR, XPS, XRD, and EDS techniques.					
32679323	1	38	theme	α-FeO	149:153	arg1	oxide/chitosan					133:146	A goethite/graphene oxide/chitosan	113:146	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent	113:188	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	1	38	theme	α-FeO	149:153	arg1	/GO/CS					158:163	α-FeO(OH)/GO/CS	149:163	α-FeO(OH)/GO/CS	149:163	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	2	39	theme	XPS	302:304	arg1	techniques					320:329	FTIR, XPS, XRD, and EDS techniques	296:329	FTIR, XPS, XRD, and EDS techniques	296:329	The composite was characterized by FTIR, XPS, XRD, and EDS techniques.					
32679323	0	40	from	solutions	49:57	arg1	removal					17:23	Highly efficient removal	0:23	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene	0:81	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene oxide/chitosan nanocomposite.					
32679323	3	41	dep	adsorption	504:513	arg1	III					499:501	III	499:501	III	499:501	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	42	theme	factors	403:409	arg1	effects					384:390	the effects	380:390	the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption	380:513	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	43	theme	kinetic	542:548	arg1	adsorption					531:540	adsorption kinetic	531:548	adsorption kinetic	531:548	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	7	44	theme	adsorption	1115:1124	arg1	289.42 mg/g					1139:1149	289.42 mg/g	1139:1149	289.42 mg/g	1139:1149	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	7	44	theme	adsorption	1115:1124	arg1	capacity					1126:1133	the maximum adsorption capacity	1103:1133	the maximum adsorption capacity	1103:1133	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	0	45	theme	III	31:33	arg1	removal					17:23	Highly efficient removal	0:23	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene	0:81	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene oxide/chitosan nanocomposite.					
32679323	4	46	theme	SO42-	793:797	arg1	concentrations					775:788	the increasing m/v and co-existing ions concentrations	735:788	the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+	735:813	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	8	47	theme	FeO	1203:1205	arg1	groups					1207:1212	FeO groups	1203:1212	FeO groups	1203:1212	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	8	48	dep	related	1172:1178	arg1	III					1155:1157	III	1155:1157	III	1155:1157	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	1	49	theme	goethite/graphene	115:131	arg1	oxide/chitosan					133:146	A goethite/graphene oxide/chitosan	113:146	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent	113:188	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	1	49	theme	goethite/graphene	115:131	arg1	/GO/CS					158:163	α-FeO(OH)/GO/CS	149:163	α-FeO(OH)/GO/CS	149:163	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	5	50	theme	pH	859:860	arg1	range					862:866	a wide pH range	852:866	a wide pH range of 3-10	852:874	As(III) adsorption remained high at a wide pH range of 3-10.					
32679323	3	51	dep	factors	403:409	arg1	time					452:455	contact time	444:455	contact time	444:455	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	51	dep	factors	403:409	arg1	temperature					480:490	temperature	480:490	temperature	480:490	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	51	dep	factors	403:409	arg1	pH					435:436	pH	435:436	pH	435:436	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	51	dep	factors	403:409	arg1	m/v					439:441	m/v	439:441	m/v	439:441	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	51	dep	factors	403:409	arg1	ions					470:473	co-existing ions	458:473	co-existing ions	458:473	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	51	dep	factors	403:409	arg1	factors					403:409	several factors	395:409	several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature)	395:491	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	3	51	dep	factors	403:409	arg1	concentration					420:432	initial concentration	412:432	initial concentration	412:432	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	5	52	theme	As	816:817	arg1	adsorption					824:833	As(III) adsorption	816:833	As(III) adsorption	816:833	As(III) adsorption remained high at a wide pH range of 3-10.					
32679323	4	53	theme	PO43-	800:804	arg1	concentrations					775:788	the increasing m/v and co-existing ions concentrations	735:788	the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+	735:813	Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO42-, PO43- and Fe3+.					
32679323	8	54	theme	As	1244:1245	arg1	ions					1252:1255	As(III) ions	1244:1255	As(III) ions	1244:1255	As(III) removal was related to -NHCO-, CO, OH, and FeO groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor.					
32679323	6	55	theme	pseudo-second-order	913:931	arg1	model					941:945	a pseudo-second-order kinetic model	911:945	a pseudo-second-order kinetic model	911:945	The adsorption was well fitted to a pseudo-second-order kinetic model and was endothermic and spontaneous.					
32679323	2	56	theme	FTIR	296:299	arg1	techniques					320:329	FTIR, XPS, XRD, and EDS techniques	296:329	FTIR, XPS, XRD, and EDS techniques	296:329	The composite was characterized by FTIR, XPS, XRD, and EDS techniques.					
32679323	7	57	dep	adsorption	1008:1017	arg1	III					1003:1005	III	1003:1005	III	1003:1005	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
32679323	0	58	theme	aqueous	41:47	arg1	solutions					49:57	aqueous solutions	41:57	aqueous solutions using goethite/graphene	41:81	Highly efficient removal of As(III) from aqueous solutions using goethite/graphene oxide/chitosan nanocomposite.					
32679323	1	59	used	used	215:218	arg2	/GO/CS					158:163	α-FeO(OH)/GO/CS	149:163	α-FeO(OH)/GO/CS	149:163	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	1	59	used	used	215:218	arg2	oxide/chitosan					133:146	A goethite/graphene oxide/chitosan	113:146	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent	113:188	A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution.					
32679323	3	60	from	effects	384:390	arg1	adsorption					504:513	As(III) adsorption	496:513	As(III) adsorption	496:513	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	2	61	theme	EDS	316:318	arg1	techniques					320:329	FTIR, XPS, XRD, and EDS techniques	296:329	FTIR, XPS, XRD, and EDS techniques	296:329	The composite was characterized by FTIR, XPS, XRD, and EDS techniques.					
32679323	10	62	contain	had	1442:1444	arg1	composite					1432:1440	this composite	1427:1440	this composite	1427:1440	Thus, this composite had great potential for removing As(III) from aqueous solutions.					
32679323	10	62	contain	had	1442:1444	arg2	potential					1452:1460	great potential	1446:1460	great potential for removing As(III) from aqueous solutions	1446:1504	Thus, this composite had great potential for removing As(III) from aqueous solutions.					
32679323	5	63	theme	3-10	871:874	arg1	range					862:866	a wide pH range	852:866	a wide pH range of 3-10	852:874	As(III) adsorption remained high at a wide pH range of 3-10.					
32679323	3	64	theme	Batch	332:336	arg1	experiments					338:348	Batch experiments	332:348	Batch experiments	332:348	Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic, equilibrium isotherm, and thermodynamics.					
32679323	7	65	theme	monolayer	1077:1085	arg1	adsorption					1087:1096	monolayer adsorption	1077:1096	monolayer adsorption	1077:1096	The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g.					
34716271	5	0	theme	LCO	841:843	arg1	site					853:856	the LCO binding site	837:856	the LCO binding site	837:856	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	4	1	theme	receptor	760:767	arg1	activation					769:778	receptor activation	760:778	receptor activation	760:778	We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity.					
34716271	6	2	theme	LCO	1073:1075	arg1	receptors					1077:1085	LCO receptors	1073:1085	LCO receptors	1073:1085	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	0	3	theme	receptors	98:106	arg1	activation					68:77	signal activation	61:77	signal activation of symbiotic plant receptors	61:106	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.					
34716271	6	4	theme	hydrophobic	990:1000	arg1	signature					1046:1054	a conserved structural signature	1023:1054	a conserved structural signature in this class of LCO receptors	1023:1085	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	6	4	theme	hydrophobic	990:1000	arg1	patch					1002:1006	the hydrophobic patch	986:1006	the hydrophobic patch	986:1006	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	1	5	theme	environmental	178:190	arg1	signals					192:198	environmental signals	178:198	environmental signals	178:198	Plants and animals use cell surface receptors to sense and interpret environmental signals.					
34716271	6	6	used	used	1114:1117	arg2	legumes					1094:1100	legumes	1094:1100	legumes that can be used for in silico predictions	1094:1143	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	3	7	theme	LCO	595:597	arg1	recognition					599:609	LCO recognition	595:609	LCO recognition	595:609	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	3	8	theme	symbiotic	615:623	arg1	function					625:632	symbiotic function	615:632	symbiotic function	615:632	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	5	9	from	signaling	956:964	arg1	plants					969:974	plants	969:974	plants	969:974	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	5	10	from	plants	969:974	arg1	activation					932:941	activation	932:941	activation of symbiotic signaling in plants	932:974	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	2	11	theme	signals	321:327	arg1	recognition					265:275	the specific recognition	252:275	the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases	252:373	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	5	12	theme	binding	845:851	arg1	site					853:856	the LCO binding site	837:856	the LCO binding site	837:856	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	3	13	theme	hydrophobic	543:553	arg1	patch					555:559	a hydrophobic patch	541:559	a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function	541:632	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	3	14	theme	essential	581:589	arg1	site					576:579	the binding site	564:579	the binding site essential for LCO recognition and symbiotic function	564:632	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	5	15	theme	signaling	956:964	arg1	activation					932:941	activation	932:941	activation of symbiotic signaling in plants	932:974	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	7	16	theme	receptor	1210:1217	arg1	activation					1219:1228	cell-surface receptor activation	1197:1228	cell-surface receptor activation by kinetic proofreading of ligands	1197:1263	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	5	17	theme	site	853:856	arg1	engineering					822:832	engineering	822:832	engineering of the LCO binding site to fine-tune ligand selectivity	822:888	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	7	18	theme	cell-surface	1197:1208	arg1	activation					1219:1228	cell-surface receptor activation	1197:1228	cell-surface receptor activation by kinetic proofreading of ligands	1197:1263	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	0	19	theme	Kinetic	0:6	arg1	proofreading					8:19	Kinetic proofreading	0:19	Kinetic proofreading of lipochitooligosaccharides	0:48	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.					
34716271	3	20	theme	lysin	495:499	arg1	ectodomains					516:526	two lysin motif receptor ectodomains	491:526	two lysin motif receptor ectodomains	491:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	3	21	theme	structural	424:433	arg1	basis					435:439	the structural basis	420:439	the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains	420:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	7	22	theme	receptor	1296:1303	arg1	engineering					1305:1315	receptor engineering	1296:1315	receptor engineering	1296:1315	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	7	23	theme	plant-microbe	1340:1352	arg1	interactions					1354:1365	plant-microbe interactions	1340:1365	plant-microbe interactions	1340:1365	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	4	24	theme	kinetic	728:734	arg1	proofreading					736:747	kinetic proofreading	728:747	kinetic proofreading	728:747	We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity.					
34716271	3	25	theme	LCO	445:447	arg1	perception					449:458	LCO perception	445:458	LCO perception from the crystal structures of two lysin motif receptor ectodomains	445:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	7	26	theme	activation	1219:1228	arg1	mechanism					1184:1192	the mechanism	1180:1192	the mechanism of cell-surface receptor activation by kinetic proofreading of ligands	1180:1263	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	2	27	theme	receptor	358:365	arg1	kinases					367:373	single-pass transmembrane receptor kinases	332:373	single-pass transmembrane receptor kinases	332:373	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	6	28	theme	in	1123:1124	arg1	predictions					1133:1143	in silico predictions	1123:1143	in silico predictions	1123:1143	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	0	29	theme	lipochitooligosaccharides	24:48	arg1	proofreading					8:19	Kinetic proofreading	0:19	Kinetic proofreading of lipochitooligosaccharides	0:48	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.					
34716271	3	30	from	patch	555:559	arg1	site					576:579	the binding site	564:579	the binding site essential for LCO recognition and symbiotic function	564:632	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	2	31	theme	transmembrane	344:356	arg1	kinases					367:373	single-pass transmembrane receptor kinases	332:373	single-pass transmembrane receptor kinases	332:373	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	3	32	theme	ectodomains	516:526	arg1	structures					477:486	the crystal structures	465:486	the crystal structures of two lysin motif receptor ectodomains	465:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	5	33	theme	binding	902:908	arg1	kinetics					910:917	binding kinetics	902:917	binding kinetics required for activation of symbiotic signaling in plants	902:974	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	6	34	theme	structural	1035:1044	arg1	patch					1002:1006	the hydrophobic patch	986:1006	the hydrophobic patch	986:1006	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	6	34	theme	structural	1035:1044	arg1	signature					1046:1054	a conserved structural signature	1023:1054	a conserved structural signature in this class of LCO receptors	1023:1085	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	2	35	theme	legume	204:209	arg1	symbiosis					211:219	legume symbiosis	204:219	legume symbiosis with nitrogen-fixing bacteria	204:249	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	2	36	theme	single-pass	332:342	arg1	kinases					367:373	single-pass transmembrane receptor kinases	332:373	single-pass transmembrane receptor kinases	332:373	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	5	37	theme	symbiotic	946:954	arg1	signaling					956:964	symbiotic signaling	946:964	symbiotic signaling in plants	946:974	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	0	38	theme	signal	61:66	arg1	activation					68:77	signal activation	61:77	signal activation of symbiotic plant receptors	61:106	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.					
34716271	6	39	theme	conserved	1025:1033	arg1	patch					1002:1006	the hydrophobic patch	986:1006	the hydrophobic patch	986:1006	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	6	39	theme	conserved	1025:1033	arg1	signature					1046:1054	a conserved structural signature	1023:1054	a conserved structural signature in this class of LCO receptors	1023:1085	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	4	40	theme	LCO	705:707	arg1	molecules					709:717	the amphiphilic LCO molecules	689:717	the amphiphilic LCO molecules	689:717	We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity.					
34716271	6	41	dep	in	1123:1124	arg1	silico					1126:1131	silico	1126:1131	silico	1126:1131	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	3	42	theme	motif	501:505	arg1	ectodomains					516:526	two lysin motif receptor ectodomains	491:526	two lysin motif receptor ectodomains	491:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	3	43	theme	crystal	469:475	arg1	structures					477:486	the crystal structures	465:486	the crystal structures of two lysin motif receptor ectodomains	465:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	6	44	from	signature	1046:1054	arg1	class					1064:1068	this class	1059:1068	this class of LCO receptors	1059:1085	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
34716271	2	45	theme	specific	256:263	arg1	recognition					265:275	the specific recognition	252:275	the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases	252:373	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	3	46	theme	receptor	507:514	arg1	ectodomains					516:526	two lysin motif receptor ectodomains	491:526	two lysin motif receptor ectodomains	491:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	5	47	theme	fine-tune	861:869	arg1	selectivity					878:888	fine-tune ligand selectivity	861:888	fine-tune ligand selectivity	861:888	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	7	48	from	potential	1283:1291	arg1	engineering					1305:1315	receptor engineering	1296:1315	receptor engineering	1296:1315	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	5	49	from	activation	932:941	arg1	plants					969:974	plants	969:974	plants	969:974	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	1	50	theme	cell	132:135	arg1	receptors					145:153	cell surface receptors	132:153	cell surface receptors to sense	132:162	Plants and animals use cell surface receptors to sense and interpret environmental signals.					
34716271	5	51	theme	ligand	871:876	arg1	selectivity					878:888	fine-tune ligand selectivity	861:888	fine-tune ligand selectivity	861:888	We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants.					
34716271	1	52	theme	surface	137:143	arg1	receptors					145:153	cell surface receptors	132:153	cell surface receptors to sense	132:162	Plants and animals use cell surface receptors to sense and interpret environmental signals.					
34716271	7	53	theme	kinetic	1233:1239	arg1	proofreading					1241:1252	kinetic proofreading	1233:1252	kinetic proofreading of ligands	1233:1263	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	2	54	theme	nitrogen-fixing	226:240	arg1	bacteria					242:249	nitrogen-fixing bacteria	226:249	nitrogen-fixing bacteria	226:249	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	7	55	from	benefits	1328:1335	arg1	interactions					1354:1365	plant-microbe interactions	1340:1365	plant-microbe interactions	1340:1365	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	2	56	theme	lipochitooligosaccharide	290:313	arg1	signals					321:327	bacterial lipochitooligosaccharide (LCO) signals	280:327	bacterial lipochitooligosaccharide (LCO) signals	280:327	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	3	57	from	structures	477:486	arg1	perception					449:458	LCO perception	445:458	LCO perception from the crystal structures of two lysin motif receptor ectodomains	445:526	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	4	58	theme	signaling	784:792	arg1	specificity					794:804	signaling specificity	784:804	signaling specificity	784:804	We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity.					
34716271	0	59	theme	plant	92:96	arg1	receptors					98:106	symbiotic plant receptors	82:106	symbiotic plant receptors	82:106	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.					
34716271	4	60	theme	amphiphilic	693:703	arg1	molecules					709:717	the amphiphilic LCO molecules	689:717	the amphiphilic LCO molecules	689:717	We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity.					
34716271	2	61	theme	bacterial	280:288	arg1	LCO					316:318	LCO	316:318	LCO	316:318	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	2	61	theme	bacterial	280:288	arg1	lipochitooligosaccharide					290:313	bacterial lipochitooligosaccharide	280:313	bacterial lipochitooligosaccharide (LCO) signals	280:327	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	3	62	theme	binding	568:574	arg1	site					576:579	the binding site	564:579	the binding site essential for LCO recognition and symbiotic function	564:632	Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function.					
34716271	4	63	theme	molecules	709:717	arg1	composition					674:684	the composition	670:684	the composition of the amphiphilic LCO molecules	670:717	We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity.					
34716271	0	64	theme	symbiotic	82:90	arg1	receptors					98:106	symbiotic plant receptors	82:106	symbiotic plant receptors	82:106	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.					
34716271	2	65	with	symbiosis	211:219	arg1	bacteria					242:249	nitrogen-fixing bacteria	226:249	nitrogen-fixing bacteria	226:249	In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility.					
34716271	7	66	theme	ligands	1257:1263	arg1	proofreading					1241:1252	kinetic proofreading	1233:1252	kinetic proofreading of ligands	1233:1263	Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.					
34716271	6	67	theme	receptors	1077:1085	arg1	class					1064:1068	this class	1059:1068	this class of LCO receptors	1059:1085	Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions.					
32184143	2	0	theme	several	503:509	arg1	advantages					511:520	several advantages	503:520	several advantages for use in regenerative medicine	503:553	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	4	1	theme	hydrogels	1011:1019	arg1	effect					896:901	a significant effect	882:901	a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels	882:1019	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	6	2	theme	tissue	1315:1320	arg1	applications					1334:1345	tissue engineering applications	1315:1345	tissue engineering applications	1315:1345	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	3	3	theme	hydrogels	616:624	arg1	design					595:600	a new design	589:600	a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au)	589:758	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	0	4	theme	engineering	88:98	arg1	applications					100:111	tissue engineering applications	81:111	tissue engineering applications	81:111	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	4	5	from	effect	896:901	arg1	swelling					946:953	equilibrium swelling	934:953	equilibrium swelling	934:953	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	5	from	effect	896:901	arg1	degradation					965:975	in vitro degradation	956:975	in vitro degradation	956:975	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	5	from	effect	896:901	arg1	morphology					922:931	the microscopic morphology	906:931	the microscopic morphology	906:931	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	5	from	effect	896:901	arg1	properties					993:1002	mechanical properties	982:1002	mechanical properties	982:1002	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	6	theme	significant	884:894	arg1	effect					896:901	a significant effect	882:901	a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels	882:1019	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	2	7	contain	possessing	427:436	arg2	structures					473:482	highly hydrated and interconnected structures	438:482	highly hydrated and interconnected structures	438:482	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	2	7	contain	possessing	427:436	arg1	scaffolds					416:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	1	8	theme	3D	249:250	arg1	biomaterials					252:263	3D biomaterials	249:263	3D biomaterials	249:263	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	6	9	theme	achieved	1208:1215	arg1	results					1217:1223	The achieved results	1204:1223	The achieved results	1204:1223	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	1	10	theme	biomaterials	252:263	arg1	design					239:244	a comprehensive design	223:244	a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration	223:373	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	2	11	theme	regenerative	533:544	arg1	medicine					546:553	regenerative medicine	533:553	regenerative medicine	533:553	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	1	12	theme	desired	349:355	arg1	regeneration					362:373	desired cell regeneration	349:373	desired cell regeneration	349:373	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	6	13	theme	new	1295:1297	arg1	biomaterial					1299:1309	a new biomaterial	1293:1309	a new biomaterial for tissue engineering applications	1293:1345	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	6	13	theme	new	1295:1297	arg1	potential					1280:1288	great potential	1274:1288	great potential	1274:1288	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	4	14	theme	mechanical	982:991	arg1	properties					993:1002	mechanical properties	982:1002	mechanical properties	982:1002	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	1	15	theme	cell	357:360	arg1	regeneration					362:373	desired cell regeneration	349:373	desired cell regeneration	349:373	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	4	16	theme	content	869:875	arg1	presence					851:858	the presence	847:858	the presence of CS-Au content	847:875	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	16	theme	content	869:875	arg1	ratios					819:824	the various molar ratios	801:824	the various molar ratios of Collagen/CNCs	801:841	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	0	17	contain	containing	47:56	arg2	gold					58:61	gold	58:61	gold	58:61	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	0	17	contain	containing	47:56	arg1	scaffold					38:45	An injectable chitosan-based hydrogel scaffold	0:45	An injectable chitosan-based hydrogel scaffold containing gold	0:61	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	4	18	theme	various	805:811	arg1	ratios					819:824	the various molar ratios	801:824	the various molar ratios of Collagen/CNCs	801:841	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	19	theme	CS-Au	863:867	arg1	content					869:875	CS-Au content	863:875	CS-Au content	863:875	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	2	20	theme	in	391:392	arg1	scaffolds					416:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	0	21	theme	injectable	3:12	arg1	scaffold					38:45	An injectable chitosan-based hydrogel scaffold	0:45	An injectable chitosan-based hydrogel scaffold containing gold	0:61	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	5	22	theme	cytotoxicity	1026:1037	arg1	analysis					1039:1046	The cytotoxicity analysis	1022:1046	The cytotoxicity analysis	1022:1046	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	4	23	theme	microscopic	910:920	arg1	morphology					922:931	the microscopic morphology	906:931	the microscopic morphology	906:931	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	2	24	theme	hydrated	445:452	arg1	structures					473:482	highly hydrated and interconnected structures	438:482	highly hydrated and interconnected structures	438:482	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	5	25	theme	cells	1197:1201	arg1	destruction					1178:1188	the destruction	1174:1188	the destruction of the cells	1174:1201	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	6	26	theme	great	1274:1278	arg1	biomaterial					1299:1309	a new biomaterial	1293:1309	a new biomaterial for tissue engineering applications	1293:1345	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	6	26	theme	great	1274:1278	arg1	potential					1280:1288	great potential	1274:1288	great potential	1274:1288	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	5	27	theme	hydrogels	1156:1164	arg1	non-toxicity					1126:1137	non-toxicity	1126:1137	non-toxicity	1126:1137	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	5	27	theme	hydrogels	1156:1164	arg1	effectiveness					1108:1120	effectiveness	1108:1120	effectiveness	1108:1120	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	2	28	theme	Injectable	376:385	arg1	scaffolds					416:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	0	29	theme	hydrogel	29:36	arg1	scaffold					38:45	An injectable chitosan-based hydrogel scaffold	0:45	An injectable chitosan-based hydrogel scaffold containing gold	0:61	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	4	30	theme	Collagen/CNCs	829:841	arg1	presence					851:858	the presence	847:858	the presence of CS-Au content	847:875	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	30	theme	Collagen/CNCs	829:841	arg1	ratios					819:824	the various molar ratios	801:824	the various molar ratios of Collagen/CNCs	801:841	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	6	31	theme	prepared	1244:1251	arg1	network					1262:1268	the prepared hydrogel network	1240:1268	the prepared hydrogel network	1240:1268	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	4	32	theme	experiments	776:786	arg1	results					765:771	The results	761:771	The results of experiments	761:786	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	0	33	theme	chitosan-based	14:27	arg1	scaffold					38:45	An injectable chitosan-based hydrogel scaffold	0:45	An injectable chitosan-based hydrogel scaffold containing gold	0:61	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	5	34	theme	developed	1146:1154	arg1	hydrogels					1156:1164	the developed hydrogels	1142:1164	the developed hydrogels	1142:1164	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	3	35	theme	aldehyde	645:652	arg1	cellulose					679:687	aldehyde modified-nanocrystalline cellulose	645:687	aldehyde modified-nanocrystalline cellulose	645:687	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	1	36	theme	tissue	127:132	arg1	engineering					134:144	tissue engineering	127:144	tissue engineering for particular sites, for example	127:178	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	1	37	theme	bone	201:204	arg1	Scaffolds					114:122	Scaffolds	114:122	Scaffolds of tissue engineering for particular sites, for example	114:178	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	1	37	theme	bone	201:204	arg1	tissues					206:212	bone tissues	201:212	bone tissues	201:212	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	6	38	contain	has	1270:1272	arg2	biomaterial					1299:1309	a new biomaterial	1293:1309	a new biomaterial for tissue engineering applications	1293:1345	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	6	38	contain	has	1270:1272	arg1	network					1262:1268	the prepared hydrogel network	1240:1268	the prepared hydrogel network	1240:1268	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	6	38	contain	has	1270:1272	arg2	potential					1280:1288	great potential	1274:1288	great potential	1274:1288	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	3	39	theme	modified-nanocrystalline	654:677	arg1	cellulose					679:687	aldehyde modified-nanocrystalline cellulose	645:687	aldehyde modified-nanocrystalline cellulose	645:687	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	2	40	theme	hydrogel	407:414	arg1	scaffolds					416:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	1	41	theme	physical	292:299	arg1	structures					301:310	physical structures	292:310	physical structures	292:310	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	4	42	theme	in	956:957	arg1	degradation					965:975	in vitro degradation	956:975	in vitro degradation	956:975	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	2	43	theme	forming	399:405	arg1	scaffolds					416:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds	376:424	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	1	44	theme	structures	301:310	arg1	aspects					281:287	all aspects	277:287	all aspects	277:287	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	5	45	theme	3T3	1074:1076	arg1	line					1083:1086	the NIH 3T3 cell line	1066:1086	the NIH 3T3 cell line	1066:1086	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	6	46	theme	hydrogel	1253:1260	arg1	network					1262:1268	the prepared hydrogel network	1240:1268	the prepared hydrogel network	1240:1268	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	1	47	theme	engineering	134:144	arg1	cardiac					188:194	cardiac	188:194	cardiac	188:194	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	1	47	theme	engineering	134:144	arg1	Scaffolds					114:122	Scaffolds	114:122	Scaffolds of tissue engineering for particular sites, for example	114:178	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	1	47	theme	engineering	134:144	arg1	nerve					181:185	nerve	181:185	nerve	181:185	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	1	47	theme	engineering	134:144	arg1	tissues					206:212	bone tissues	201:212	bone tissues	201:212	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	2	48	theme	interconnected	458:471	arg1	structures					473:482	highly hydrated and interconnected structures	438:482	highly hydrated and interconnected structures	438:482	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	5	49	dep	effectiveness	1108:1120	arg1	the					1104:1106	the	1104:1106	the	1104:1106	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	3	50	theme	gold	715:718	arg1	Collagen/ADH-CNCs/CS-Au					735:757	Collagen/ADH-CNCs/CS-Au	735:757	Collagen/ADH-CNCs/CS-Au	735:757	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	3	50	theme	gold	715:718	arg1	nanoparticles					720:732	gold nanoparticles	715:732	gold nanoparticles (Collagen/ADH-CNCs/CS-Au)	715:758	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	5	51	theme	NIH	1070:1072	arg1	3T3					1074:1076	NIH 3T3	1070:1076	the NIH 3T3 cell line	1066:1086	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	3	52	theme	new	591:593	arg1	design					595:600	a new design	589:600	a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au)	589:758	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	4	53	contain	have	877:880	arg2	effect					896:901	a significant effect	882:901	a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels	882:1019	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	53	contain	have	877:880	arg1	presence					851:858	the presence	847:858	the presence of CS-Au content	847:875	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	4	53	contain	have	877:880	arg1	ratios					819:824	the various molar ratios	801:824	the various molar ratios of Collagen/CNCs	801:841	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	1	54	theme	chemical	316:323	arg1	composition					325:335	chemical composition	316:335	chemical composition	316:335	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	4	55	theme	equilibrium	934:944	arg1	swelling					946:953	equilibrium swelling	934:953	equilibrium swelling	934:953	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	2	56	from	use	526:528	arg1	medicine					546:553	regenerative medicine	533:553	regenerative medicine	533:553	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	1	57	theme	particular	150:159	arg1	sites					161:165	particular sites	150:165	particular sites	150:165	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	6	58	theme	engineering	1322:1332	arg1	applications					1334:1345	tissue engineering applications	1315:1345	tissue engineering applications	1315:1345	The achieved results suggested that the prepared hydrogel network has great potential as a new biomaterial for tissue engineering applications.					
32184143	5	59	theme	cell	1078:1081	arg1	line					1083:1086	the NIH 3T3 cell line	1066:1086	the NIH 3T3 cell line	1066:1086	The cytotoxicity analysis was performed for the NIH 3T3 cell line, which displays the effectiveness and non-toxicity of the developed hydrogels towards the destruction of the cells.					
32184143	4	60	theme	molar	813:817	arg1	ratios					819:824	the various molar ratios	801:824	the various molar ratios of Collagen/CNCs	801:841	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32184143	0	61	theme	tissue	81:86	arg1	applications					100:111	tissue engineering applications	81:111	tissue engineering applications	81:111	An injectable chitosan-based hydrogel scaffold containing gold nanoparticles for tissue engineering applications.					
32184143	3	62	theme	injectable	605:614	arg1	hydrogels					616:624	injectable hydrogels	605:624	injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au)	605:758	In this study, we have developed a new design of injectable hydrogels based on collagen, aldehyde modified-nanocrystalline cellulose, and chitosan loaded with gold nanoparticles (Collagen/ADH-CNCs/CS-Au).					
32184143	1	63	theme	comprehensive	225:237	arg1	design					239:244	a comprehensive design	223:244	a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration	223:373	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	1	64	theme	composition	325:335	arg1	aspects					281:287	all aspects	277:287	all aspects	277:287	Scaffolds of tissue engineering for particular sites, for example, nerve, cardiac, and bone tissues, require a comprehensive design of 3D biomaterials that covers all aspects of physical structures and chemical composition, needed for desired cell regeneration.					
32184143	2	65	dep	in	391:392	arg1	situ					394:397	situ	394:397	situ	394:397	Injectable and in situ forming hydrogel scaffolds, possessing highly hydrated and interconnected structures, have demonstrated several advantages for use in regenerative medicine.					
32184143	4	66	dep	in	956:957	arg1	vitro					959:963	vitro	959:963	vitro	959:963	The results of experiments exposed that the various molar ratios of Collagen/CNCs and the presence of CS-Au content have a significant effect on the microscopic morphology, equilibrium swelling, in vitro degradation, and mechanical properties of the hydrogels.					
32448574	11	0	theme	RPG-fed	1770:1776	arg1	cows					1778:1781	RPG-fed cows	1770:1781	RPG-fed cows	1770:1781	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	4	1	theme	milk	867:870	arg1	yield					872:876	milk yield	867:876	milk yield	867:876	Weekly body weight, milk composition, and fecal pH were recorded until 28 d in milk (DIM), and milk yield was recorded through 105 DIM.					
32448574	2	2	theme	305-d	362:366	arg1	yield					399:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	10	3	dep	lower	1554:1558	arg1	acids					1580:1584	nonesterified fatty acids	1560:1584	lower nonesterified fatty acids (28%)	1554:1590	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	10	4	theme	β-hydroxybutyrate	1614:1630	arg1	concentration					1510:1522	increased concentration	1500:1522	increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows	1500:1652	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	6	5	theme	MIXED	1034:1038	arg1	procedure					1040:1048	the MIXED procedure	1030:1048	the MIXED procedure	1030:1048	Data were analyzed using repeated measures in the MIXED procedure (SAS Institute Inc., Cary, NC) with previous 305ME as a covariate.					
32448574	10	6	theme	plasma	1416:1421	arg1	concentrations					1431:1444	plasma glucose concentrations	1416:1444	plasma glucose concentrations	1416:1444	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	11	7	theme	lipopolysaccharide-binding	1797:1822	arg1	protein					1824:1830	decreased lipopolysaccharide-binding protein and haptoglobin concentrations	1787:1861	protein	1824:1830	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	10	8	theme	lower	1597:1601	arg1	%					1635:1635	24%	1633:1635	24%	1633:1635	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	10	8	theme	lower	1597:1601	arg1	β-hydroxybutyrate					1614:1630	lower postpartum β-hydroxybutyrate	1597:1630	lower postpartum β-hydroxybutyrate (24%)	1597:1636	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	1	9	theme	glucose	178:184	arg1	supplementation					192:206	rumen-protected glucose (RPG) supplementation	162:206	rumen-protected glucose (RPG) supplementation	162:206	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	12	10	theme	concomitant	2153:2163	arg1	reduction					2165:2173	a concomitant reduction	2151:2173	a concomitant reduction in acute phase proteins without changing milk production and composition	2151:2246	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	3	11	theme	respective	637:646	arg1	treatments					656:665	their respective dietary treatments	631:665	their respective dietary treatments	631:665	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	6	12	theme	repeated	1009:1016	arg1	measures					1018:1025	repeated measures	1009:1025	repeated measures	1009:1025	Data were analyzed using repeated measures in the MIXED procedure (SAS Institute Inc., Cary, NC) with previous 305ME as a covariate.					
32448574	6	12	theme	repeated	1009:1016	arg1	covariate					1106:1114	a covariate	1104:1114	a covariate	1104:1114	Data were analyzed using repeated measures in the MIXED procedure (SAS Institute Inc., Cary, NC) with previous 305ME as a covariate.					
32448574	2	13	theme	multiparous	320:330	arg1	cows					332:335	Fifty-two multiparous cows	310:335	Fifty-two multiparous cows	310:335	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	1	14	theme	RPG	187:189	arg1	supplementation					192:206	rumen-protected glucose (RPG) supplementation	162:206	rumen-protected glucose (RPG) supplementation	162:206	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	6	15	dep	Cary	1071:1074	arg1	NC					1077:1078	NC	1077:1078	NC	1077:1078	Data were analyzed using repeated measures in the MIXED procedure (SAS Institute Inc., Cary, NC) with previous 305ME as a covariate.					
32448574	12	16	theme	acute	2178:2182	arg1	proteins					2190:2197	acute phase proteins	2178:2197	acute phase proteins without changing milk production and composition	2178:2246	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	1	17	from	biomarkers	273:282	arg1	cows					304:307	transition dairy cows	287:307	transition dairy cows	287:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	1	18	theme	supplementation	192:206	arg1	effects					151:157	the effects	147:157	the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows	147:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	7	19	theme	Fecal	1117:1121	arg1	pH					1123:1124	Fecal pH	1117:1124	Fecal pH	1117:1124	Fecal pH was similar between treatments and decreased (0.6 units) postpartum.					
32448574	1	20	theme	milk	211:214	arg1	production					216:225	milk production	211:225	milk production	211:225	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	11	21	dep	protein	1824:1830	arg1	%					1873:1873	31 and 27%	1864:1873	31 and 27%	1864:1873	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	10	22	theme	RPG-fed	1641:1647	arg1	cows					1649:1652	RPG-fed cows	1641:1652	RPG-fed cows	1641:1652	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	1	23	from	metabolism	244:253	arg1	cows					304:307	transition dairy cows	287:307	transition dairy cows	287:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	12	24	theme	inflammation	1990:2001	arg1	biomarkers					1945:1954	some biomarkers	1940:1954	some biomarkers of post-absorptive energetics and inflammation	1940:2001	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	7	25	dep	decreased	1161:1169	arg1	units					1176:1180	0.6 units	1172:1180	0.6 units	1172:1180	Fecal pH was similar between treatments and decreased (0.6 units) postpartum.					
32448574	4	26	theme	body	779:782	arg1	weight					784:789	Weekly body weight	772:789	Weekly body weight	772:789	Weekly body weight, milk composition, and fecal pH were recorded until 28 d in milk (DIM), and milk yield was recorded through 105 DIM.					
32448574	8	27	theme	Dry	1195:1197	arg1	intake					1206:1211	Dry matter intake	1195:1211	Dry matter intake pre- and postpartum	1195:1231	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	2	28	theme	postpartum	596:605	arg1	postpartum					596:605	dry matter postpartum	585:605	dry matter postpartum	585:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	28	theme	postpartum	596:605	arg1	%					557:557	pre-fresh 5.3%	544:557	pre-fresh 5.3% of dry matter	544:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	28	theme	postpartum	596:605	arg1	matter					566:571	dry matter	562:571	dry matter	562:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	28	theme	postpartum	596:605	arg1	%					580:580	6.0%	577:580	6.0% of dry matter postpartum	577:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	29	contain	containing	528:537	arg1	treatments					471:480	2 iso-energetic and iso-nitrogenous treatments	435:480	2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	435:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	29	contain	containing	528:537	arg1	diet					523:526	a diet	521:526	(2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	517:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	29	contain	containing	528:537	arg2	RPG					539:541	RPG	539:541	RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	539:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	3	30	theme	relative	684:691	arg1	d					672:672	d -21 to 28	672:682	d -21 to 28 relative to calving	672:702	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	3	31	theme	matter	713:718	arg1	intake					720:725	dry matter intake	709:725	dry matter intake	709:725	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	12	32	theme	insulin	2082:2088	arg1	concentrations					2130:2143	increased insulin and decreased nonesterified fatty acids concentrations	2072:2143	increased insulin and decreased nonesterified fatty acids concentrations	2072:2143	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	10	33	theme	circulating	1527:1537	arg1	insulin					1539:1545	circulating insulin	1527:1545	circulating insulin (27%)	1527:1551	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	10	33	theme	circulating	1527:1537	arg1	%					1550:1550	27%	1548:1550	27%	1548:1550	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	6	34	dep	using	1003:1007	arg1	Cary					1071:1074	Cary	1071:1074	Cary	1071:1074	Data were analyzed using repeated measures in the MIXED procedure (SAS Institute Inc., Cary, NC) with previous 305ME as a covariate.					
32448574	0	35	theme	transition	68:77	arg1	cows					85:88	transition dairy cows	68:88	transition dairy cows	68:88	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	12	36	theme	periparturient	2014:2027	arg1	period					2029:2034	the periparturient period	2010:2034	the periparturient period	2010:2034	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	1	37	from	effects	151:157	arg1	production					216:225	milk production	211:225	milk production	211:225	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	1	37	from	effects	151:157	arg1	metabolism					244:253	post-absorptive metabolism	228:253	post-absorptive metabolism	228:253	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	1	37	from	effects	151:157	arg1	biomarkers					273:282	inflammatory biomarkers	260:282	inflammatory biomarkers in transition dairy cows	260:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	12	38	theme	energetics	1975:1984	arg1	biomarkers					1945:1954	some biomarkers	1940:1954	some biomarkers of post-absorptive energetics and inflammation	1940:2001	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	0	39	theme	cows	85:88	arg1	response					56:63	inflammatory response	43:63	inflammatory response	43:63	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	0	39	theme	cows	85:88	arg1	metabolism					10:19	Energetic metabolism	0:19	Energetic metabolism	0:19	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	0	39	theme	cows	85:88	arg1	production					27:36	milk production	22:36	milk production	22:36	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	5	40	theme	Blood	908:912	arg1	samples					914:920	Blood samples	908:920	Blood samples	908:920	Blood samples were collected on d -7, 3, 7, 14, and 28 relative to calving.					
32448574	11	41	theme	lipopolysaccharide-binding	1676:1701	arg1	protein					1703:1709	circulating lipopolysaccharide-binding protein	1664:1709	circulating lipopolysaccharide-binding protein	1664:1709	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	2	42	theme	dry	562:564	arg1	matter					566:571	dry matter	562:571	dry matter	562:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	43	theme	Supplemental	1914:1925	arg1	RPG					1927:1929	Supplemental RPG	1914:1929	Supplemental RPG	1914:1929	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	0	44	theme	rumen-protected	94:108	arg1	glucose					110:116	rumen-protected glucose	94:116	rumen-protected glucose	94:116	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	3	45	dep	d	672:672	arg1	to					678:679	to	678:679	to	678:679	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	2	46	theme	dry	585:587	arg1	postpartum					596:605	dry matter postpartum	585:605	dry matter postpartum	585:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	0	47	theme	Energetic	0:8	arg1	metabolism					10:19	Energetic metabolism	0:19	Energetic metabolism	0:19	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	1	48	theme	dairy	298:302	arg1	cows					304:307	transition dairy cows	287:307	transition dairy cows	287:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	2	49	dep	=	610:610	arg1	postpartum					596:605	dry matter postpartum	585:605	dry matter postpartum	585:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	49	dep	=	610:610	arg1	%					557:557	pre-fresh 5.3%	544:557	pre-fresh 5.3% of dry matter	544:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	49	dep	=	610:610	arg1	matter					566:571	dry matter	562:571	dry matter	562:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	49	dep	=	610:610	arg1	%					580:580	6.0%	577:580	6.0% of dry matter postpartum	577:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	50	theme	treatments	471:480	arg1	treatments					471:480	2 iso-energetic and iso-nitrogenous treatments	435:480	2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	435:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	50	theme	treatments	471:480	arg1	diet					523:526	a diet	521:526	(2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	517:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	50	theme	treatments	471:480	arg1	diet					495:498	(1) control diet (CON; n = 26)	483:512	(1) control diet (CON; n = 26)	483:512	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	50	theme	treatments	471:480	arg1	1					430:430	1	430:430	1	430:430	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	51	theme	acids	2124:2128	arg1	concentrations					2130:2143	increased insulin and decreased nonesterified fatty acids concentrations	2072:2143	increased insulin and decreased nonesterified fatty acids concentrations	2072:2143	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	11	52	theme	decreased	1787:1795	arg1	protein					1824:1830	decreased lipopolysaccharide-binding protein and haptoglobin concentrations	1787:1861	protein	1824:1830	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	2	53	theme	previous	353:360	arg1	yield					399:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	54	theme	post-absorptive	1959:1973	arg1	energetics					1975:1984	post-absorptive energetics	1959:1984	post-absorptive energetics	1959:1984	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	9	55	theme	Milk	1313:1316	arg1	fat					1318:1320	Milk fat	1313:1320	Milk fat	1313:1320	Milk fat, protein, and lactose concentration were similar for both treatments.					
32448574	10	56	theme	Blood	1392:1396	arg1	nitrogen					1403:1410	Blood urea nitrogen	1392:1410	Blood urea nitrogen	1392:1410	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	10	57	theme	nonesterified	1560:1572	arg1	acids					1580:1584	nonesterified fatty acids	1560:1584	lower nonesterified fatty acids (28%)	1554:1590	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	1	58	theme	post-absorptive	228:242	arg1	metabolism					244:253	post-absorptive metabolism	228:253	post-absorptive metabolism	228:253	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	12	59	theme	nonesterified	2104:2116	arg1	acids					2124:2128	decreased nonesterified fatty acids	2094:2128	decreased nonesterified fatty acids	2094:2128	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	2	60	theme	mature-equivalent	368:384	arg1	yield					399:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	5	61	theme	relative	963:970	arg1	d					940:940	d -7, 3, 7, 14, and 28	940:961	d -7, 3, 7, 14, and 28 relative to calving	940:981	Blood samples were collected on d -7, 3, 7, 14, and 28 relative to calving.					
32448574	9	62	theme	lactose	1336:1342	arg1	concentration					1344:1356	lactose concentration	1336:1356	lactose concentration	1336:1356	Milk fat, protein, and lactose concentration were similar for both treatments.					
32448574	11	63	theme	circulating	1664:1674	arg1	protein					1703:1709	circulating lipopolysaccharide-binding protein	1664:1709	circulating lipopolysaccharide-binding protein	1664:1709	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	1	64	theme	inflammatory	260:271	arg1	biomarkers					273:282	inflammatory biomarkers	260:282	inflammatory biomarkers in transition dairy cows	260:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	2	65	dep	=	508:508	arg1	CON					501:503	CON	501:503	CON	501:503	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	66	theme	milk	2216:2219	arg1	production					2221:2230	milk production	2216:2230	milk production	2216:2230	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	12	67	theme	increased	2072:2080	arg1	concentrations					2130:2143	increased insulin and decreased nonesterified fatty acids concentrations	2072:2143	increased insulin and decreased nonesterified fatty acids concentrations	2072:2143	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	10	68	theme	postpartum	1603:1612	arg1	%					1635:1635	24%	1633:1635	24%	1633:1635	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	10	68	theme	postpartum	1603:1612	arg1	β-hydroxybutyrate					1614:1630	lower postpartum β-hydroxybutyrate	1597:1630	lower postpartum β-hydroxybutyrate (24%)	1597:1636	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	1	69	theme	rumen-protected	162:176	arg1	supplementation					192:206	rumen-protected glucose (RPG) supplementation	162:206	rumen-protected glucose (RPG) supplementation	162:206	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	7	70	theme	decreased	1161:1169	arg1	postpartum					1183:1192	decreased (0.6 units) postpartum	1161:1192	decreased (0.6 units) postpartum	1161:1192	Fecal pH was similar between treatments and decreased (0.6 units) postpartum.					
32448574	11	71	contain	had	1783:1785	arg2	protein					1824:1830	decreased lipopolysaccharide-binding protein and haptoglobin concentrations	1787:1861	protein	1824:1830	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	11	71	contain	had	1783:1785	arg1	cows					1778:1781	RPG-fed cows	1770:1781	RPG-fed cows	1770:1781	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	11	71	contain	had	1783:1785	arg2	concentrations					1848:1861	decreased lipopolysaccharide-binding protein and haptoglobin concentrations	1787:1861	concentrations	1848:1861	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	2	72	theme	pre-fresh	544:552	arg1	postpartum					596:605	dry matter postpartum	585:605	dry matter postpartum	585:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	72	theme	pre-fresh	544:552	arg1	%					557:557	pre-fresh 5.3%	544:557	pre-fresh 5.3% of dry matter	544:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	72	theme	pre-fresh	544:552	arg1	matter					566:571	dry matter	562:571	dry matter	562:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	73	theme	phase	2184:2188	arg1	proteins					2190:2197	acute phase proteins	2178:2197	acute phase proteins without changing milk production and composition	2178:2246	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	3	74	theme	dietary	648:654	arg1	treatments					656:665	their respective dietary treatments	631:665	their respective dietary treatments	631:665	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	2	75	theme	Fifty-two	310:318	arg1	cows					332:335	Fifty-two multiparous cows	310:335	Fifty-two multiparous cows	310:335	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	76	dep	diet	523:526	arg1	2					518:518	2	518:518	2	518:518	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	4	77	theme	milk	792:795	arg1	composition					797:807	milk composition	792:807	milk composition	792:807	Weekly body weight, milk composition, and fecal pH were recorded until 28 d in milk (DIM), and milk yield was recorded through 105 DIM.					
32448574	10	78	theme	increased	1500:1508	arg1	concentration					1510:1522	increased concentration	1500:1522	increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows	1500:1652	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	2	79	dep	RPG	539:541	arg1	=					610:610	=	610:610	=	610:610	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	8	80	theme	matter	1199:1204	arg1	intake					1206:1211	Dry matter intake	1195:1211	Dry matter intake pre- and postpartum	1195:1231	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	1	81	from	production	216:225	arg1	cows					304:307	transition dairy cows	287:307	transition dairy cows	287:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	8	82	dep	yield	1275:1279	arg1	was					1266:1268	was	1266:1268	was	1266:1268	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	8	82	dep	yield	1275:1279	arg1	DIM					1308:1310	the first 28 or 105 DIM	1288:1310	the first 28 or 105 DIM	1288:1310	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	8	82	dep	yield	1275:1279	arg1	as					1263:1264	as	1263:1264	as	1263:1264	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	4	83	theme	Weekly	772:777	arg1	weight					784:789	Weekly body weight	772:789	Weekly body weight	772:789	Weekly body weight, milk composition, and fecal pH were recorded until 28 d in milk (DIM), and milk yield was recorded through 105 DIM.					
32448574	8	84	theme	pre-	1213:1216	arg1	intake					1206:1211	Dry matter intake	1195:1211	Dry matter intake pre- and postpartum	1195:1231	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	10	85	theme	insulin	1539:1545	arg1	concentration					1510:1522	increased concentration	1500:1522	increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows	1500:1652	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	2	86	theme	control	487:493	arg1	treatments					471:480	2 iso-energetic and iso-nitrogenous treatments	435:480	2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	435:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	86	theme	control	487:493	arg1	diet					495:498	(1) control diet (CON; n = 26)	483:512	(1) control diet (CON; n = 26)	483:512	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	8	87	theme	postpartum	1222:1231	arg1	intake					1206:1211	Dry matter intake	1195:1211	Dry matter intake pre- and postpartum	1195:1231	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	0	88	theme	dairy	79:83	arg1	cows					85:88	transition dairy cows	68:88	transition dairy cows	68:88	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	2	89	dep	treatments	471:480	arg1	treatments					471:480	2 iso-energetic and iso-nitrogenous treatments	435:480	2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	435:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	89	dep	treatments	471:480	arg1	diet					523:526	a diet	521:526	(2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	517:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	89	dep	treatments	471:480	arg1	diet					495:498	(1) control diet (CON; n = 26)	483:512	(1) control diet (CON; n = 26)	483:512	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	3	90	theme	same	759:762	arg1	period					764:769	the same period	755:769	the same period	755:769	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	12	91	from	reduction	2165:2173	arg1	proteins					2190:2197	acute phase proteins	2178:2197	acute phase proteins without changing milk production and composition	2178:2246	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	3	92	theme	dry	709:711	arg1	intake					720:725	dry matter intake	709:725	dry matter intake	709:725	Cows received their respective dietary treatments from d -21 to 28 relative to calving, and dry matter intake was calculated daily during the same period.					
32448574	2	93	theme	matter	566:571	arg1	postpartum					596:605	dry matter postpartum	585:605	dry matter postpartum	585:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	93	theme	matter	566:571	arg1	%					557:557	pre-fresh 5.3%	544:557	pre-fresh 5.3% of dry matter	544:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	93	theme	matter	566:571	arg1	matter					566:571	dry matter	562:571	dry matter	562:571	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	93	theme	matter	566:571	arg1	%					580:580	6.0%	577:580	6.0% of dry matter postpartum	577:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	4	94	theme	fecal	814:818	arg1	pH					820:821	fecal pH	814:821	fecal pH	814:821	Weekly body weight, milk composition, and fecal pH were recorded until 28 d in milk (DIM), and milk yield was recorded through 105 DIM.					
32448574	2	95	theme	matter	589:594	arg1	postpartum					596:605	dry matter postpartum	585:605	dry matter postpartum	585:605	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	10	96	from	concentration	1510:1522	arg1	cows					1649:1652	RPG-fed cows	1641:1652	RPG-fed cows	1641:1652	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	2	97	theme	iso-energetic	437:449	arg1	treatments					471:480	2 iso-energetic and iso-nitrogenous treatments	435:480	2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	435:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	97	theme	iso-energetic	437:449	arg1	diet					523:526	a diet	521:526	(2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	517:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	97	theme	iso-energetic	437:449	arg1	diet					495:498	(1) control diet (CON; n = 26)	483:512	(1) control diet (CON; n = 26)	483:512	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	8	98	theme	milk	1270:1273	arg1	yield					1275:1279	milk yield	1270:1279	milk yield	1270:1279	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	2	99	theme	305ME	392:396	arg1	yield					399:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	0	100	theme	milk	22:25	arg1	production					27:36	milk production	22:36	milk production	22:36	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	1	101	theme	transition	287:296	arg1	cows					304:307	transition dairy cows	287:307	transition dairy cows	287:307	Objectives were to evaluate the effects of rumen-protected glucose (RPG) supplementation on milk production, post-absorptive metabolism, and inflammatory biomarkers in transition dairy cows.					
32448574	11	102	theme	haptoglobin	1836:1846	arg1	concentrations					1848:1861	decreased lipopolysaccharide-binding protein and haptoglobin concentrations	1787:1861	concentrations	1848:1861	Overall, circulating lipopolysaccharide-binding protein and haptoglobin did not differ by treatment, but at 7 DIM, RPG-fed cows had decreased lipopolysaccharide-binding protein and haptoglobin concentrations (31 and 27%, respectively) compared with controls.					
32448574	10	103	theme	lower	1554:1558	arg1	concentration					1510:1522	increased concentration	1500:1522	increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows	1500:1652	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	8	104	theme	first	1292:1296	arg1	DIM					1308:1310	the first 28 or 105 DIM	1288:1310	the first 28 or 105 DIM	1288:1310	Dry matter intake pre- and postpartum were unaffected by treatment, as was milk yield during the first 28 or 105 DIM.					
32448574	6	105	theme	previous	1086:1093	arg1	305ME					1095:1099	previous 305ME	1086:1099	previous 305ME	1086:1099	Data were analyzed using repeated measures in the MIXED procedure (SAS Institute Inc., Cary, NC) with previous 305ME as a covariate.					
32448574	0	106	theme	inflammatory	43:54	arg1	response					56:63	inflammatory response	43:63	inflammatory response	43:63	Energetic metabolism, milk production, and inflammatory response of transition dairy cows fed rumen-protected glucose.					
32448574	2	107	theme	iso-nitrogenous	455:469	arg1	treatments					471:480	2 iso-energetic and iso-nitrogenous treatments	435:480	2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	435:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	107	theme	iso-nitrogenous	455:469	arg1	diet					523:526	a diet	521:526	(2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26)	517:614	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	107	theme	iso-nitrogenous	455:469	arg1	diet					495:498	(1) control diet (CON; n = 26)	483:512	(1) control diet (CON; n = 26)	483:512	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	10	108	theme	urea	1398:1401	arg1	nitrogen					1403:1410	Blood urea nitrogen	1392:1410	Blood urea nitrogen	1392:1410	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	2	109	dep	diet	495:498	arg1	=					508:508	=	508:508	=	508:508	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	2	109	dep	diet	495:498	arg1	1					484:484	1	484:484	1	484:484	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	110	theme	decreased	2094:2102	arg1	acids					2124:2128	decreased nonesterified fatty acids	2094:2128	decreased nonesterified fatty acids	2094:2128	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	10	111	theme	fatty	1574:1578	arg1	acids					1580:1584	nonesterified fatty acids	1560:1584	lower nonesterified fatty acids (28%)	1554:1590	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
32448574	2	112	theme	milk	386:389	arg1	yield					399:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	previous 305-d mature-equivalent milk (305ME) yield	353:403	Fifty-two multiparous cows were blocked by previous 305-d mature-equivalent milk (305ME) yield and randomly assigned to 1 of 2 iso-energetic and iso-nitrogenous treatments: (1) control diet (CON; n = 26) or (2) a diet containing RPG (pre-fresh 5.3% of dry matter and 6.0% of dry matter postpartum; n = 26).					
32448574	12	113	theme	fatty	2118:2122	arg1	acids					2124:2128	decreased nonesterified fatty acids	2094:2128	decreased nonesterified fatty acids	2094:2128	Supplemental RPG improved some biomarkers of post-absorptive energetics and inflammation during the periparturient period, changes primarily characterized by increased insulin and decreased nonesterified fatty acids concentrations, with a concomitant reduction in acute phase proteins without changing milk production and composition.					
32448574	10	114	theme	glucose	1423:1429	arg1	concentrations					1431:1444	plasma glucose concentrations	1416:1444	plasma glucose concentrations	1416:1444	Blood urea nitrogen and plasma glucose concentrations were unaffected by treatment; however, results showed increased concentration of circulating insulin (27%), lower nonesterified fatty acids (28%), and lower postpartum β-hydroxybutyrate (24%) in RPG-fed cows.					
33298215	9	0	theme	Bifidobacterium	1591:1605	arg1	producers					1613:1621	SCFA producers	1608:1621	SCFA producers	1608:1621	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	9	0	theme	Bifidobacterium	1591:1605	arg1	levels					1624:1629	Lactobacillus and Bifidobacterium (SCFA producers) levels	1573:1629	Lactobacillus and Bifidobacterium (SCFA producers) levels	1573:1629	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	10	1	theme	microbiota	1736:1745	arg1	composition					1747:1757	gut microbiota composition	1732:1757	gut microbiota composition	1732:1757	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	10	2	theme	intake	1704:1709	arg1	importance					1676:1685	the importance	1672:1685	the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance	1672:1786	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	1	3	theme	high	158:161	arg1	doses					163:167	high doses	158:167	high doses of radio/chemotherapy treatment	158:199	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	8	4	contain	had	1231:1233	arg2	permeability					1248:1259	worsened gut permeability	1235:1259	worsened gut permeability	1235:1259	For instance, mice that received the LF diet had worsened gut permeability, compared with mice that received the normal diet and mucositis.					
33298215	8	4	contain	had	1231:1233	arg1	mice					1200:1203	mice	1200:1203	mice that received the LF diet	1200:1229	For instance, mice that received the LF diet had worsened gut permeability, compared with mice that received the normal diet and mucositis.					
33298215	10	5	theme	gut	1732:1734	arg1	composition					1747:1757	gut microbiota composition	1732:1757	gut microbiota composition	1732:1757	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	9	6	theme	LF	1352:1353	arg1	effects					1330:1336	The effects	1326:1336	The effects of the HF and LF diets	1326:1359	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	3	7	theme	high-fibre	473:482	arg1	diet					489:492	a high-fibre (HF) diet	471:492	a high-fibre (HF) diet	471:492	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	8	8	theme	worsened	1235:1242	arg1	permeability					1248:1259	worsened gut permeability	1235:1259	worsened gut permeability	1235:1259	For instance, mice that received the LF diet had worsened gut permeability, compared with mice that received the normal diet and mucositis.					
33298215	0	9	from	Effects	0:6	arg1	mucositis					56:64	chemotherapy-induced mucositis	35:64	chemotherapy-induced mucositis in murine model	35:80	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	2	10	theme	SCFA	294:297	arg1	production					299:308	SCFA production	294:308	SCFA production	294:308	Soluble fibres, like pectin, increase SCFA production, which play a role in gut homoeostasis and inflammation suppression.					
33298215	5	11	theme	histopathological	873:889	arg1	parameters					891:900	improved histopathological parameters	864:900	improved histopathological parameters	864:900	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	6	12	theme	mucositis	1044:1052	arg1	model					1054:1058	the mucositis model	1040:1058	the mucositis model	1040:1058	Furthermore, the HF diet decreased intestinal permeability induced in the mucositis model when compared with the control group.					
33298215	0	13	from	mucositis	56:64	arg1	model					76:80	murine model	69:80	murine model	69:80	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	5	14	theme	cells	847:851	arg1	influx					853:858	decreased immune cells influx	830:858	decreased immune cells influx	830:858	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	3	15	theme	chemotherapy-induced	497:516	arg1	mucositis					518:526	chemotherapy-induced mucositis	497:526	chemotherapy-induced mucositis	497:526	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	4	16	theme	C57/BL6	547:553	arg1	mice					555:558	C57/BL6 mice	547:558	C57/BL6 mice	547:558	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	4	17	theme	challenging	645:655	arg1	mucositis					635:643	mucositis challenging	635:655	mucositis challenging with irinotecan (75 mg/kg)	635:682	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	9	18	theme	gut	1500:1502	arg1	inflammation					1504:1515	gut inflammation	1500:1515	gut inflammation	1500:1515	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	4	19	dep	diets	611:615	arg1	HF					587:588	control (AIN93M), HF, low/zero fibre (LF) diets	569:615	HF	587:588	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	4	20	theme	fibre	600:604	arg1	diets					611:615	control (AIN93M), HF, low/zero fibre (LF) diets	569:615	diets	611:615	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	4	20	theme	fibre	600:604	arg1	LF					607:608	LF	607:608	LF	607:608	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	10	21	theme	homoeostasis	1763:1774	arg1	maintenance					1776:1786	homoeostasis maintenance	1763:1786	homoeostasis maintenance	1763:1786	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	4	22	theme	drinking	728:735	arg1	water					737:741	drinking water	728:741	drinking water 5 d	728:745	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	3	23	theme	diet	489:492	arg1	effect					461:466	the effect	457:466	the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis	457:526	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	1	24	theme	radio/chemotherapy	172:189	arg1	treatment					191:199	radio/chemotherapy treatment	172:199	radio/chemotherapy treatment	172:199	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	4	25	theme	d	745:745	arg1	water					737:741	drinking water	728:741	drinking water 5 d	728:745	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	9	26	theme	Lactobacillus	1573:1585	arg1	producers					1613:1621	SCFA producers	1608:1621	SCFA producers	1608:1621	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	9	26	theme	Lactobacillus	1573:1585	arg1	levels					1624:1629	Lactobacillus and Bifidobacterium (SCFA producers) levels	1573:1629	Lactobacillus and Bifidobacterium (SCFA producers) levels	1573:1629	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	5	27	theme	HF	815:816	arg1	diet					818:821	the HF diet	811:821	the HF diet	811:821	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	10	28	theme	maintenance	1776:1786	arg1	modulation					1718:1727	the modulation	1714:1727	the modulation of gut microbiota composition and homoeostasis maintenance	1714:1786	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	1	29	theme	treatment	191:199	arg1	doses					163:167	high doses	158:167	high doses of radio/chemotherapy treatment	158:199	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	4	30	theme	5	743:743	arg1	d					745:745	d	745:745	d	745:745	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	3	31	from	effect	461:466	arg1	mucositis					518:526	chemotherapy-induced mucositis	497:526	chemotherapy-induced mucositis	497:526	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	3	32	theme	murine	533:538	arg1	model					540:544	a murine model	531:544	a murine model	531:544	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	8	33	theme	normal	1299:1304	arg1	diet					1306:1309	the normal diet	1295:1309	the normal diet	1295:1309	For instance, mice that received the LF diet had worsened gut permeability, compared with mice that received the normal diet and mucositis.					
33298215	0	34	theme	fibre	19:23	arg1	intake					25:30	dietary fibre intake	11:30	dietary fibre intake	11:30	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	2	35	theme	inflammation	353:364	arg1	suppression					366:376	inflammation suppression	353:376	inflammation suppression	353:376	Soluble fibres, like pectin, increase SCFA production, which play a role in gut homoeostasis and inflammation suppression.					
33298215	8	36	theme	gut	1244:1246	arg1	permeability					1248:1259	worsened gut permeability	1235:1259	worsened gut permeability	1235:1259	For instance, mice that received the LF diet had worsened gut permeability, compared with mice that received the normal diet and mucositis.					
33298215	10	37	theme	dietary	1690:1696	arg1	intake					1704:1709	dietary fibre intake	1690:1709	dietary fibre intake	1690:1709	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	0	38	theme	dietary	11:17	arg1	intake					25:30	dietary fibre intake	11:30	dietary fibre intake	11:30	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	8	39	theme	LF	1223:1224	arg1	diet					1226:1229	the LF diet	1219:1229	the LF diet	1219:1229	For instance, mice that received the LF diet had worsened gut permeability, compared with mice that received the normal diet and mucositis.					
33298215	10	40	theme	fibre	1698:1702	arg1	intake					1704:1709	dietary fibre intake	1690:1709	dietary fibre intake	1690:1709	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	5	41	theme	decreased	830:838	arg1	influx					853:858	decreased immune cells influx	830:858	decreased immune cells influx	830:858	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	3	42	theme	pectin	404:409	arg1	properties					390:399	the properties	386:399	the properties of pectin	386:409	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	9	43	theme	HF	1345:1346	arg1	effects					1330:1336	The effects	1326:1336	The effects of the HF and LF diets	1326:1359	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	5	44	theme	normal	957:962	arg1	diet					964:967	the normal diet	953:967	the normal diet	953:967	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	9	45	theme	LF	1424:1425	arg1	diet					1427:1430	the LF diet	1420:1430	the LF diet	1420:1430	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	3	46	theme	present	427:433	arg1	study					435:439	the present study	423:439	the present study	423:439	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	0	47	theme	intake	25:30	arg1	Effects					0:6	Effects	0:6	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model	0:80	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	3	48	theme	study	435:439	arg1	aim					416:418	the aim	412:418	the aim of the present study	412:439	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	9	49	theme	SCFA	1608:1611	arg1	producers					1613:1621	SCFA producers	1608:1621	SCFA producers	1608:1621	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	9	49	theme	SCFA	1608:1611	arg1	levels					1624:1629	Lactobacillus and Bifidobacterium (SCFA producers) levels	1573:1629	Lactobacillus and Bifidobacterium (SCFA producers) levels	1573:1629	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	4	50	theme	mucositis	771:779	arg1	induction					781:789	the mucositis induction	767:789	the mucositis induction	767:789	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	2	51	theme	gut	332:334	arg1	homoeostasis					336:347	gut homoeostasis	332:347	gut homoeostasis	332:347	Soluble fibres, like pectin, increase SCFA production, which play a role in gut homoeostasis and inflammation suppression.					
33298215	0	52	theme	chemotherapy-induced	35:54	arg1	mucositis					56:64	chemotherapy-induced mucositis	35:64	chemotherapy-induced mucositis in murine model	35:80	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	10	53	theme	composition	1747:1757	arg1	modulation					1718:1727	the modulation	1714:1727	the modulation of gut microbiota composition and homoeostasis maintenance	1714:1786	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	9	54	theme	Enterobacteriaceae	1456:1473	arg1	levels					1446:1451	the levels	1442:1451	the levels of Enterobacteriaceae, a group associated with gut inflammation	1442:1515	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	6	55	theme	intestinal	1005:1014	arg1	permeability					1016:1027	intestinal permeability	1005:1027	intestinal permeability induced in the mucositis model	1005:1058	Furthermore, the HF diet decreased intestinal permeability induced in the mucositis model when compared with the control group.					
33298215	5	56	theme	improved	864:871	arg1	parameters					891:900	improved histopathological parameters	864:900	improved histopathological parameters	864:900	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	1	57	theme	gastrointestinal	119:134	arg1	mucosa					136:141	the gastrointestinal mucosa	115:141	the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment	115:199	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	4	58	theme	control	569:575	arg1	diets					611:615	control (AIN93M), HF, low/zero fibre (LF) diets	569:615	diets	611:615	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	4	58	theme	control	569:575	arg1	LF					607:608	LF	607:608	LF	607:608	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	9	59	dep	HF	1345:1346	arg1	diets					1355:1359	diets	1355:1359	diets	1355:1359	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	4	60	dep	control	569:575	arg1	AIN93M					578:583	AIN93M	578:583	AIN93M	578:583	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	5	61	theme	immune	840:845	arg1	cells					847:851	immune cells	840:851	decreased immune cells influx	830:858	Mice that received the HF diet showed decreased immune cells influx and improved histopathological parameters in the intestine, compared with mice that received the normal diet.					
33298215	1	62	theme	mucosa	136:141	arg1	Mucositis					83:91	Mucositis	83:91	Mucositis	83:91	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	1	62	theme	mucosa	136:141	arg1	inflammation					99:110	an inflammation	96:110	an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment	96:199	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	1	63	theme	antineoplasic	233:245	arg1	therapy					247:253	antineoplasic therapy	233:253	antineoplasic therapy	233:253	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	0	64	theme	murine	69:74	arg1	model					76:80	murine model	69:80	murine model	69:80	Effects of dietary fibre intake in chemotherapy-induced mucositis in murine model.					
33298215	7	65	theme	gut	1168:1170	arg1	permeability					1172:1183	gut permeability	1168:1183	gut permeability	1168:1183	This effect was not observed for acetate alone, which did not improve gut permeability.					
33298215	6	66	theme	control	1083:1089	arg1	group					1091:1095	the control group	1079:1095	the control group	1079:1095	Furthermore, the HF diet decreased intestinal permeability induced in the mucositis model when compared with the control group.					
33298215	6	67	theme	HF	987:988	arg1	diet					990:993	the HF diet	983:993	the HF diet	983:993	Furthermore, the HF diet decreased intestinal permeability induced in the mucositis model when compared with the control group.					
33298215	4	68	theme	low/zero	591:598	arg1	diets					611:615	control (AIN93M), HF, low/zero fibre (LF) diets	569:615	diets	611:615	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	4	68	theme	low/zero	591:598	arg1	LF					607:608	LF	607:608	LF	607:608	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	1	69	theme	therapy	247:253	arg1	interruption					217:228	interruption	217:228	interruption of antineoplasic therapy	217:253	Mucositis is an inflammation of the gastrointestinal mucosa resulting from high doses of radio/chemotherapy treatment and may lead to interruption of antineoplasic therapy.					
33298215	10	70	from	importance	1676:1685	arg1	modulation					1718:1727	the modulation	1714:1727	the modulation of gut microbiota composition and homoeostasis maintenance	1714:1786	In conclusion, the results demonstrated the importance of dietary fibre intake in the modulation of gut microbiota composition and homoeostasis maintenance during mucositis in this model.					
33298215	4	71	with	mucositis	635:643	arg1	irinotecan					662:671	irinotecan	662:671	irinotecan (75 mg/kg)	662:682	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	4	71	with	mucositis	635:643	arg1	mg/kg					677:681	75 mg/kg	674:681	75 mg/kg	674:681	C57/BL6 mice received control (AIN93M), HF, low/zero fibre (LF) diets for 10 d prior to mucositis challenging with irinotecan (75 mg/kg), or they were treated with acetate added to drinking water 5 d prior to and during the mucositis induction.					
33298215	2	72	theme	Soluble	256:262	arg1	fibres					264:269	Soluble fibres	256:269	Soluble fibres	256:269	Soluble fibres, like pectin, increase SCFA production, which play a role in gut homoeostasis and inflammation suppression.					
33298215	9	73	theme	intestinal	1388:1397	arg1	microbiota					1399:1408	the intestinal microbiota	1384:1408	the intestinal microbiota	1384:1408	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33298215	3	74	theme	HF	485:486	arg1	diet					489:492	a high-fibre (HF) diet	471:492	a high-fibre (HF) diet	471:492	Due to the properties of pectin, the aim of the present study was to evaluate the effect of a high-fibre (HF) diet on chemotherapy-induced mucositis in a murine model.					
33298215	9	75	theme	HF	1530:1531	arg1	diet					1533:1536	the HF diet	1526:1536	the HF diet	1526:1536	The effects of the HF and LF diets were shown to modulate the intestinal microbiota, in which the LF diet increased the levels of Enterobacteriaceae, a group associated with gut inflammation, whereas the HF diet decreased this group and increased Lactobacillus and Bifidobacterium (SCFA producers) levels.					
33513424	0	0	theme	hydrogels	84:92	arg1	fabrication					39:49	the fabrication	35:49	the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture	35:122	A freeze-thawing method applied to the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture.					
33513424	2	1	theme	X-ray	461:465	arg1	diffraction					467:477	X-ray diffraction	461:477	X-ray diffraction (XRD)	461:483	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	2	1	theme	X-ray	461:465	arg1	XRD					480:482	XRD	480:482	XRD	480:482	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	7	2	from	cells	1044:1048	arg1	hydrogels					1067:1075	the composite hydrogels	1053:1075	the composite hydrogels	1053:1075	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	2	3	theme	interactions	329:340	arg1	presence					317:324	The presence	313:324	The presence of interactions, changes in crystallinity, and thermal behaviour	313:389	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	0	4	theme	alcohol	76:82	arg1	hydrogels					84:92	3-d curdlan/polyvinyl alcohol hydrogels	54:92	3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture	54:122	A freeze-thawing method applied to the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture.					
33513424	1	5	theme	innovative	142:151	arg1	hydrogel					163:170	an innovative composite hydrogel	139:170	an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure	139:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	7	6	contain	had	1077:1079	arg1	cells					1044:1048	the cells	1040:1048	the cells in the composite hydrogels	1040:1075	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	7	6	contain	had	1077:1079	arg2	cytocompatibility					1086:1102	good cytocompatibility	1081:1102	good cytocompatibility	1081:1102	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	4	7	theme	mechanical	701:710	arg1	strength					712:719	a greater mechanical strength	691:719	a greater mechanical strength	691:719	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	6	8	theme	cytotoxic	930:938	arg1	effect					940:945	no cytotoxic effect	927:945	no cytotoxic effect	927:945	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	2	9	dep	thermogravimetry	490:505	arg1	TGA					508:510	TGA	508:510	TGA	508:510	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	2	9	dep	thermogravimetry	490:505	arg1	DTG					516:518	DTG	516:518	DTG	516:518	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	7	10	theme	composite	1057:1065	arg1	hydrogels					1067:1075	the composite hydrogels	1053:1075	the composite hydrogels	1053:1075	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	2	11	from	presence	317:324	arg1	crystallinity					354:366	crystallinity	354:366	crystallinity	354:366	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	4	12	theme	greater	693:699	arg1	strength					712:719	a greater mechanical strength	691:719	a greater mechanical strength	691:719	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	8	13	theme	present	1146:1152	arg1	work					1154:1157	the present work	1142:1157	the present work	1142:1157	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	0	14	theme	cell	111:114	arg1	culture					116:122	cell culture	111:122	cell culture	111:122	A freeze-thawing method applied to the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture.					
33513424	5	15	theme	hydrogels	855:863	arg1	swelling					816:823	swelling	816:823	swelling	816:823	PVA content affects the swelling and water retention of CD/PVA hydrogels.					
33513424	5	15	theme	hydrogels	855:863	arg1	retention					835:843	water retention	829:843	water retention	829:843	PVA content affects the swelling and water retention of CD/PVA hydrogels.					
33513424	2	16	dep	Fourier	412:418	arg1	transform					420:428	transform	420:428	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively	420:533	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	1	17	theme	composite	153:161	arg1	hydrogel					163:170	an innovative composite hydrogel	139:170	an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure	139:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	2	18	from	interactions	329:340	arg1	crystallinity					354:366	crystallinity	354:366	crystallinity	354:366	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	6	19	theme	fibroblast	960:969	arg1	cells					976:980	the mouse fibroblast L929 cells	950:980	the mouse fibroblast L929 cells	950:980	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	9	20	theme	soft	1320:1323	arg1	tissue					1325:1330	soft tissue	1320:1330	soft tissue engineering application	1320:1354	CD/PVA hydrogels highlight the value of this system for cell adhesion and proliferation, and further soft tissue engineering application.					
33513424	8	21	theme	growth	1185:1190	arg1	environment					1192:1202	an ideal cell growth environment	1171:1202	an ideal cell growth environment	1171:1202	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	8	21	theme	growth	1185:1190	arg1	scaffold					1209:1216	a scaffold	1207:1216	a scaffold	1207:1216	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	0	22	theme	freeze-thawing	2:15	arg1	method					17:22	A freeze-thawing method	0:22	A freeze-thawing method	0:22	A freeze-thawing method applied to the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture.					
33513424	9	23	theme	cell	1275:1278	arg1	adhesion					1280:1287	cell adhesion	1275:1287	cell adhesion	1275:1287	CD/PVA hydrogels highlight the value of this system for cell adhesion and proliferation, and further soft tissue engineering application.					
33513424	4	24	contain	had	687:689	arg2	strength					712:719	a greater mechanical strength	691:719	a greater mechanical strength	691:719	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	4	24	contain	had	687:689	arg1	hydrogel					678:685	the composite hydrogel	664:685	the composite hydrogel	664:685	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	9	25	theme	system	1264:1269	arg1	value					1250:1254	the value	1246:1254	the value of this system for cell adhesion and proliferation, and further soft tissue engineering application	1246:1354	CD/PVA hydrogels highlight the value of this system for cell adhesion and proliferation, and further soft tissue engineering application.					
33513424	1	26	theme	3-d	238:240	arg1	structure					250:258	a 3-d network structure	236:258	a 3-d network structure	236:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	2	27	dep	transform	420:428	arg1	infrared					430:437	infrared	430:437	infrared	430:437	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	2	28	from	changes	343:349	arg1	crystallinity					354:366	crystallinity	354:366	crystallinity	354:366	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	4	29	theme	composite	668:676	arg1	hydrogel					678:685	the composite hydrogel	664:685	the composite hydrogel	664:685	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	1	30	theme	network	242:248	arg1	structure					250:258	a 3-d network structure	236:258	a 3-d network structure	236:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	6	31	contain	have	922:925	arg1	hydrogels					912:920	CD/PVA hydrogels	905:920	CD/PVA hydrogels	905:920	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	6	31	contain	have	922:925	arg2	effect					940:945	no cytotoxic effect	927:945	no cytotoxic effect	927:945	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	6	32	theme	CD/PVA	905:910	arg1	hydrogels					912:920	CD/PVA hydrogels	905:920	CD/PVA hydrogels	905:920	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	3	33	theme	electron	597:604	arg1	SEM					618:620	SEM	618:620	SEM	618:620	The morphology of the hydrogels was investigated by scanning electron microscopy (SEM).					
33513424	3	33	theme	electron	597:604	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	The morphology of the hydrogels was investigated by scanning electron microscopy (SEM).					
33513424	5	34	theme	CD/PVA	848:853	arg1	hydrogels					855:863	CD/PVA hydrogels	848:863	CD/PVA hydrogels	848:863	PVA content affects the swelling and water retention of CD/PVA hydrogels.					
33513424	5	35	theme	water	829:833	arg1	retention					835:843	water retention	829:843	water retention	829:843	PVA content affects the swelling and water retention of CD/PVA hydrogels.					
33513424	7	36	theme	double-staining	993:1007	arg1	experiment					1009:1018	The AO/EB double-staining experiment	983:1018	The AO/EB double-staining experiment	983:1018	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	5	37	theme	PVA	792:794	arg1	content					796:802	PVA content	792:802	PVA content	792:802	PVA content affects the swelling and water retention of CD/PVA hydrogels.					
33513424	5	38	dep	swelling	816:823	arg1	the					812:814	the	812:814	the	812:814	PVA content affects the swelling and water retention of CD/PVA hydrogels.					
33513424	2	39	theme	behaviour	381:389	arg1	presence					317:324	The presence	313:324	The presence of interactions, changes in crystallinity, and thermal behaviour	313:389	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	2	40	from	crystallinity	354:366	arg1	presence					317:324	The presence	313:324	The presence of interactions, changes in crystallinity, and thermal behaviour	313:389	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	2	41	theme	thermal	373:379	arg1	behaviour					381:389	thermal behaviour	373:389	thermal behaviour	373:389	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	7	42	theme	good	1081:1084	arg1	cytocompatibility					1086:1102	good cytocompatibility	1081:1102	good cytocompatibility	1081:1102	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	6	43	theme	assay	887:891	arg1	results					870:876	The results	866:876	The results of CCK-8 assay	866:891	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	3	44	theme	scanning	588:595	arg1	SEM					618:620	SEM	618:620	SEM	618:620	The morphology of the hydrogels was investigated by scanning electron microscopy (SEM).					
33513424	3	44	theme	scanning	588:595	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	The morphology of the hydrogels was investigated by scanning electron microscopy (SEM).					
33513424	4	45	theme	test	778:781	arg1	results					783:789	tensile test results	770:789	tensile test results	770:789	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	4	46	theme	concentration	649:661	arg1	increase					633:640	the increase	629:640	the increase of PVA concentration	629:661	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	8	47	theme	ideal	1174:1178	arg1	environment					1192:1202	an ideal cell growth environment	1171:1202	an ideal cell growth environment	1171:1202	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	8	47	theme	ideal	1174:1178	arg1	scaffold					1209:1216	a scaffold	1207:1216	a scaffold	1207:1216	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	6	48	theme	CCK-8	881:885	arg1	assay					887:891	CCK-8 assay	881:891	CCK-8 assay	881:891	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	7	49	theme	AO/EB	987:991	arg1	experiment					1009:1018	The AO/EB double-staining experiment	983:1018	The AO/EB double-staining experiment	983:1018	The AO/EB double-staining experiment further proved that the cells in the composite hydrogels had good cytocompatibility.					
33513424	4	50	theme	tensile	770:776	arg1	results					783:789	tensile test results	770:789	tensile test results	770:789	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	4	51	theme	PVA	645:647	arg1	concentration					649:661	PVA concentration	645:661	PVA concentration	645:661	With the increase of PVA concentration, the composite hydrogel had a greater mechanical strength while remaining remarkably ductile as evinced by tensile test results.					
33513424	1	52	theme	freeze-thaw	289:299	arg1	processing					301:310	freeze-thaw processing	289:310	freeze-thaw processing	289:310	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	1	53	with	hydrogels	221:229	arg1	structure					250:258	a 3-d network structure	236:258	a 3-d network structure	236:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	1	54	theme	/polyvinyl	196:205	arg1	PVA					216:218	PVA	216:218	PVA	216:218	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	1	54	theme	/polyvinyl	196:205	arg1	alcohol					207:213	curdlan (CD)/polyvinyl alcohol	184:213	curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure	184:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	9	55	theme	CD/PVA	1219:1224	arg1	hydrogels					1226:1234	CD/PVA hydrogels	1219:1234	CD/PVA hydrogels	1219:1234	CD/PVA hydrogels highlight the value of this system for cell adhesion and proliferation, and further soft tissue engineering application.					
33513424	2	56	from	behaviour	381:389	arg1	crystallinity					354:366	crystallinity	354:366	crystallinity	354:366	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	8	57	theme	cell	1180:1183	arg1	environment					1192:1202	an ideal cell growth environment	1171:1202	an ideal cell growth environment	1171:1202	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	8	57	theme	cell	1180:1183	arg1	scaffold					1209:1216	a scaffold	1207:1216	a scaffold	1207:1216	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
33513424	1	58	theme	alcohol	207:213	arg1	hydrogels					221:229	curdlan (CD)/polyvinyl alcohol (PVA) hydrogels	184:229	curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure	184:258	In this work, an innovative composite hydrogel composed of curdlan (CD)/polyvinyl alcohol (PVA) hydrogels with a 3-d network structure was successfully prepared by freeze-thaw processing.					
33513424	0	59	theme	curdlan/polyvinyl	58:74	arg1	hydrogels					84:92	3-d curdlan/polyvinyl alcohol hydrogels	54:92	3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture	54:122	A freeze-thawing method applied to the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture.					
33513424	2	60	theme	changes	343:349	arg1	presence					317:324	The presence	313:324	The presence of interactions, changes in crystallinity, and thermal behaviour	313:389	The presence of interactions, changes in crystallinity, and thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetry (TGA and DTG), respectively.					
33513424	6	61	theme	mouse	954:958	arg1	cells					976:980	the mouse fibroblast L929 cells	950:980	the mouse fibroblast L929 cells	950:980	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	0	62	theme	3-d	54:56	arg1	hydrogels					84:92	3-d curdlan/polyvinyl alcohol hydrogels	54:92	3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture	54:122	A freeze-thawing method applied to the fabrication of 3-d curdlan/polyvinyl alcohol hydrogels as scaffolds for cell culture.					
33513424	3	63	theme	hydrogels	558:566	arg1	morphology					540:549	The morphology	536:549	The morphology of the hydrogels	536:566	The morphology of the hydrogels was investigated by scanning electron microscopy (SEM).					
33513424	6	64	theme	L929	971:974	arg1	cells					976:980	the mouse fibroblast L929 cells	950:980	the mouse fibroblast L929 cells	950:980	The results of CCK-8 assay showed that CD/PVA hydrogels have no cytotoxic effect on the mouse fibroblast L929 cells.					
33513424	8	65	theme	porous	1109:1114	arg1	biohydrogels					1116:1127	The porous biohydrogels	1105:1127	The porous biohydrogels developed in the present work	1105:1157	The porous biohydrogels developed in the present work can provide an ideal cell growth environment as a scaffold.					
34560993	1	0	theme	biomedical	265:274	arg1	fields					276:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	2	1	theme	d-α-tocopheryl	532:545	arg1	glycol					560:565	d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	532:587	d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	532:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	4	2	theme	caveolae-mediated	803:819	arg1	endocytosis					821:831	adsorptive-mediated and caveolae-mediated endocytosis	779:831	endocytosis	821:831	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	0	3	theme	gemcitabine	108:118	arg1	effects					97:103	chemotherapeutic effects	80:103	chemotherapeutic effects	80:103	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	0	3	theme	gemcitabine	108:118	arg1	bioavailability					60:74	the oral bioavailability	51:74	the oral bioavailability	51:74	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	6	4	theme	pharmacodynamic	977:991	arg1	studies					993:999	pharmacodynamic studies	977:999	pharmacodynamic studies	977:999	In pharmacodynamic studies, the formulation reduced tumour size 3.1-fold compared with the drug solution.					
34560993	5	5	theme	nanocomplexes	908:920	arg1	bioavailability					885:899	the oral drug bioavailability	871:899	the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution	871:971	Pharmacokinetic studies demonstrated the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution.					
34560993	7	6	theme	TMC	1107:1109	arg1	nanocomplexes					1120:1132	TMC modified nanocomplexes	1107:1132	TMC modified nanocomplexes	1107:1132	The data demonstrates that TMC modified nanocomplexes can enhance gemcitabine oral bioavailability and promote the anticancer efficacy.					
34560993	5	7	theme	oral	875:878	arg1	bioavailability					885:899	the oral drug bioavailability	871:899	the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution	871:971	Pharmacokinetic studies demonstrated the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution.					
34560993	2	8	theme	poly	461:464	arg1	nanoparticles					491:503	poly(lactic-co-glycolic acid) nanoparticles	461:503	poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	461:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	8	theme	poly	461:464	arg1	NPs					511:513	PLGA NPs	506:513	PLGA NPs	506:513	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	9	theme	antitumour	385:394	arg1	effects					396:402	antitumour effects	385:402	antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	385:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	0	10	theme	chitosan	12:19	arg1	nano-complexes					28:41	N-trimethyl chitosan coated nano-complexes	0:41	N-trimethyl chitosan coated nano-complexes	0:41	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	4	11	theme	drug	685:688	arg1	studies					705:711	drug transportation studies	685:711	drug transportation studies	685:711	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	0	12	theme	N-trimethyl	0:10	arg1	nano-complexes					28:41	N-trimethyl chitosan coated nano-complexes	0:41	N-trimethyl chitosan coated nano-complexes	0:41	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	6	13	theme	drug	1065:1068	arg1	solution					1070:1077	the drug solution	1061:1077	the drug solution	1061:1077	In pharmacodynamic studies, the formulation reduced tumour size 3.1-fold compared with the drug solution.					
34560993	7	14	theme	anticancer	1195:1204	arg1	efficacy					1206:1213	the anticancer efficacy	1191:1213	the anticancer efficacy	1191:1213	The data demonstrates that TMC modified nanocomplexes can enhance gemcitabine oral bioavailability and promote the anticancer efficacy.					
34560993	4	15	theme	intestinal	758:767	arg1	cells					769:773	the intestinal cells	754:773	the intestinal cells	754:773	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	5	16	theme	drug	959:962	arg1	solution					964:971	drug solution	959:971	drug solution	959:971	Pharmacokinetic studies demonstrated the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution.					
34560993	5	17	theme	drug	880:883	arg1	bioavailability					885:899	the oral drug bioavailability	871:899	the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution	871:971	Pharmacokinetic studies demonstrated the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution.					
34560993	1	18	theme	N-trimethyl	121:131	arg1	polymer					169:175	a multifunctional polymer	151:175	a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields	151:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	1	18	theme	N-trimethyl	121:131	arg1	TMC					143:145	TMC	143:145	TMC	143:145	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	1	18	theme	N-trimethyl	121:131	arg1	chitosan					133:140	N-trimethyl chitosan	121:140	N-trimethyl chitosan (TMC)	121:146	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	1	19	theme	various	197:203	arg1	forms					218:222	various nanoparticle forms	197:222	various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields	197:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	0	20	theme	coated	21:26	arg1	nano-complexes					28:41	N-trimethyl chitosan coated nano-complexes	0:41	N-trimethyl chitosan coated nano-complexes	0:41	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	4	21	dep	nanocomplexes	726:738	arg1	permeate					740:747	permeate	740:747	permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis	740:831	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	1	22	theme	nanoparticle	205:216	arg1	forms					218:222	various nanoparticle forms	197:222	various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields	197:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	5	23	theme	Pharmacokinetic	834:848	arg1	studies					850:856	Pharmacokinetic studies	834:856	Pharmacokinetic studies	834:856	Pharmacokinetic studies demonstrated the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution.					
34560993	7	24	theme	oral	1158:1161	arg1	bioavailability					1163:1177	gemcitabine oral bioavailability	1146:1177	gemcitabine oral bioavailability	1146:1177	The data demonstrates that TMC modified nanocomplexes can enhance gemcitabine oral bioavailability and promote the anticancer efficacy.					
34560993	3	25	theme	composite	600:608	arg1	design					610:615	A central composite design	590:615	A central composite design	590:615	A central composite design was applied to achieve the optimal formulation.					
34560993	2	26	theme	gemcitabine	407:417	arg1	effects					396:402	antitumour effects	385:402	antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	385:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	26	theme	gemcitabine	407:417	arg1	bioavailability					359:373	the oral bioavailability	350:373	the oral bioavailability	350:373	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	27	theme	polyethylene	547:558	arg1	glycol					560:565	d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	532:587	d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	532:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	1	28	from	forms	218:222	arg1	fields					276:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	2	29	theme	lactic-co-glycolic	466:483	arg1	poly					461:464	poly	461:464	poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	461:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	29	theme	lactic-co-glycolic	466:483	arg1	acid					485:488	lactic-co-glycolic acid	466:488	lactic-co-glycolic acid	466:488	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	30	dep	glycol	560:565	arg1	TPGS					583:586	TPGS	583:586	TPGS	583:586	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	30	dep	glycol	560:565	arg1	succinate					572:580	succinate	572:580	d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	532:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	3	31	theme	optimal	644:650	arg1	formulation					652:662	the optimal formulation	640:662	the optimal formulation	640:662	A central composite design was applied to achieve the optimal formulation.					
34560993	7	32	theme	modified	1111:1118	arg1	nanocomplexes					1120:1132	TMC modified nanocomplexes	1107:1132	TMC modified nanocomplexes	1107:1132	The data demonstrates that TMC modified nanocomplexes can enhance gemcitabine oral bioavailability and promote the anticancer efficacy.					
34560993	0	33	theme	oral	55:58	arg1	bioavailability					60:74	the oral bioavailability	51:74	the oral bioavailability	51:74	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	6	34	theme	tumour	1026:1031	arg1	size					1033:1036	tumour size	1026:1036	tumour size	1026:1036	In pharmacodynamic studies, the formulation reduced tumour size 3.1-fold compared with the drug solution.					
34560993	7	35	theme	gemcitabine	1146:1156	arg1	bioavailability					1163:1177	gemcitabine oral bioavailability	1146:1177	gemcitabine oral bioavailability	1146:1177	The data demonstrates that TMC modified nanocomplexes can enhance gemcitabine oral bioavailability and promote the anticancer efficacy.					
34560993	3	36	theme	central	592:598	arg1	design					610:615	A central composite design	590:615	A central composite design	590:615	A central composite design was applied to achieve the optimal formulation.					
34560993	4	37	theme	transportation	690:703	arg1	studies					705:711	drug transportation studies	685:711	drug transportation studies	685:711	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	4	38	theme	adsorptive-mediated	779:797	arg1	endocytosis					821:831	adsorptive-mediated and caveolae-mediated endocytosis	779:831	endocytosis	821:831	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	1	39	used	used	189:192	arg2	chitosan					133:140	N-trimethyl chitosan	121:140	N-trimethyl chitosan (TMC)	121:146	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	1	39	used	used	189:192	arg2	polymer					169:175	a multifunctional polymer	151:175	a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields	151:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	1	40	theme	multifunctional	153:167	arg1	polymer					169:175	a multifunctional polymer	151:175	a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields	151:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	1	40	theme	multifunctional	153:167	arg1	chitosan					133:140	N-trimethyl chitosan	121:140	N-trimethyl chitosan (TMC)	121:146	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	2	41	used	used	307:310	arg2	TMC					299:301	TMC	299:301	TMC	299:301	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	41	used	used	307:310	arg2	adjuvant					330:337	adjuvant	330:337	adjuvant	330:337	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	1	42	theme	pharmaceutical	231:244	arg1	fields					276:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
34560993	0	43	theme	chemotherapeutic	80:95	arg1	effects					97:103	chemotherapeutic effects	80:103	chemotherapeutic effects	80:103	N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.					
34560993	4	44	theme	Cellular	665:672	arg1	uptake					674:679	Cellular uptake	665:679	Cellular uptake	665:679	Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis.					
34560993	2	45	theme	PLGA	506:509	arg1	nanoparticles					491:503	poly(lactic-co-glycolic acid) nanoparticles	461:503	poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)	461:587	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	45	theme	PLGA	506:509	arg1	NPs					511:513	PLGA NPs	506:513	PLGA NPs	506:513	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	2	46	theme	oral	354:357	arg1	bioavailability					359:373	the oral bioavailability	350:373	the oral bioavailability	350:373	In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS).					
34560993	1	47	theme	nutraceutical	247:259	arg1	fields					276:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	the pharmaceutical, nutraceutical and biomedical fields	227:281	N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields.					
32470587	3	0	from	size	423:426	arg1	nanoparticles					384:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	0	from	size	423:426	arg1	small					414:418	small	414:418	small	414:418	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	0	from	size	423:426	arg1	stable					496:501	stable	496:501	stable	496:501	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	0	from	size	423:426	arg1	spherical					403:411	spherical	403:411	spherical	403:411	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	6	1	theme	higher	877:882	arg1	strength					892:899	higher tensile strength	877:899	higher tensile strength (TS)	877:904	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	1	theme	higher	877:882	arg1	TS					902:903	TS	902:903	TS	902:903	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	7	2	theme	release	952:958	arg1	rate					960:963	The LEO release rate	944:963	The LEO release rate	944:963	The LEO release rate increased with decreasing GCC:CS-LEO ratio.					
32470587	8	3	theme	maximum	1038:1044	arg1	rate					1058:1061	a maximum LEO release rate	1036:1061	a maximum LEO release rate of 83.6 ± 9.7%	1036:1076	At GCC:CS-LEO ratio = 7:3, a maximum LEO release rate of 83.6 ± 9.7% was achieved over 15 days.					
32470587	1	4	theme	lemon	191:195	arg1	LEO					212:214	LEO	212:214	LEO	212:214	Encapsulation effectively delays the volatilization of lemon essential oil (LEO).					
32470587	1	4	theme	lemon	191:195	arg1	oil					207:209	lemon essential oil	191:209	lemon essential oil (LEO)	191:215	Encapsulation effectively delays the volatilization of lemon essential oil (LEO).					
32470587	3	5	from	spherical	403:411	arg1	58 ± 9 nm					429:437	58 ± 9 nm	429:437	58 ± 9 nm	429:437	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	5	from	spherical	403:411	arg1	size					423:426	size	423:426	size (58 ± 9 nm)	423:438	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	7	6	theme	LEO	948:950	arg1	rate					960:963	The LEO release rate	944:963	The LEO release rate	944:963	The LEO release rate increased with decreasing GCC:CS-LEO ratio.					
32470587	5	7	theme	scanning	714:721	arg1	SEM					744:746	SEM	744:746	SEM	744:746	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	7	theme	scanning	714:721	arg1	microscope					732:741	scanning electron microscope	714:741	scanning electron microscope (SEM)	714:747	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	2	8	dep	CS	347:348	arg1	LEO					361:363	LEO	361:363	LEO	361:363	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	2	8	dep	CS	347:348	arg1	CS					358:359	CS	358:359	CS	358:359	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	2	8	dep	CS	347:348	arg1	TPP					350:352	TPP	350:352	TPP	350:352	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	6	9	theme	collagen	801:808	arg1	films					816:820	grass carp collagen (GCC) films	790:820	grass carp collagen (GCC) films	790:820	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	5	10	theme	composite	606:614	arg1	films					616:620	The composite films	602:620	The composite films	602:620	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	4	11	theme	FTIR	531:534	arg1	spectra					536:542	FTIR spectra	531:542	FTIR spectra	531:542	FTIR spectra indicated that they were fully encapsulated in the films.					
32470587	3	12	contain	had	441:443	arg1	small					414:418	small	414:418	small	414:418	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	12	contain	had	441:443	arg1	stable					496:501	stable	496:501	stable	496:501	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	12	contain	had	441:443	arg2	index					466:470	a low polydispersity index	445:470	a low polydispersity index (0.15)	445:477	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	12	contain	had	441:443	arg1	nanoparticles					384:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	12	contain	had	441:443	arg2	0.15					473:476	0.15	473:476	0.15	473:476	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	12	contain	had	441:443	arg1	spherical					403:411	spherical	403:411	spherical	403:411	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	6	13	from	break	932:936	arg1	EB					939:940	EB	939:940	EB	939:940	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	13	from	break	932:936	arg1	permeability					858:869	lower oxygen permeability	845:869	lower oxygen permeability (OP)	845:874	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	13	from	break	932:936	arg1	strength					892:899	higher tensile strength	877:899	higher tensile strength (TS)	877:904	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	13	from	break	932:936	arg1	elongation					918:927	higher elongation	911:927	higher elongation at break (EB)	911:941	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	13	from	break	932:936	arg1	TS					902:903	TS	902:903	TS	902:903	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	13	from	break	932:936	arg1	OP					872:873	OP	872:873	OP	872:873	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	1	14	theme	essential	197:205	arg1	LEO					212:214	LEO	212:214	LEO	212:214	Encapsulation effectively delays the volatilization of lemon essential oil (LEO).					
32470587	1	14	theme	essential	197:205	arg1	oil					207:209	lemon essential oil	191:209	lemon essential oil (LEO)	191:215	Encapsulation effectively delays the volatilization of lemon essential oil (LEO).					
32470587	0	15	theme	food	120:123	arg1	packaging					125:133	food packaging	120:133	food packaging	120:133	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	6	16	theme	grass	790:794	arg1	GCC					811:813	GCC	811:813	GCC	811:813	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	16	theme	grass	790:794	arg1	collagen					801:808	grass carp collagen	790:808	grass carp collagen (GCC) films	790:820	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	7	17	theme	GCC	991:993	arg1	ratio					1002:1006	GCC:CS-LEO ratio	991:1006	GCC:CS-LEO ratio	991:1006	The LEO release rate increased with decreasing GCC:CS-LEO ratio.					
32470587	1	18	theme	oil	207:209	arg1	volatilization					173:186	the volatilization	169:186	the volatilization of lemon essential oil (LEO)	169:215	Encapsulation effectively delays the volatilization of lemon essential oil (LEO).					
32470587	6	19	theme	lower	845:849	arg1	OP					872:873	OP	872:873	OP	872:873	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	19	theme	lower	845:849	arg1	permeability					858:869	lower oxygen permeability	845:869	lower oxygen permeability (OP)	845:874	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	5	20	theme	force	690:694	arg1	microscopy					696:705	atomic force microscopy	683:705	atomic force microscopy (AFM)	683:711	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	20	theme	force	690:694	arg1	AFM					708:710	AFM	708:710	AFM	708:710	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	2	21	theme	Tween-80	319:326	arg1	concentrations					328:341	Tween-80 concentrations	319:341	Tween-80 concentrations	319:341	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	6	22	theme	carp	796:799	arg1	GCC					811:813	GCC	811:813	GCC	811:813	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	22	theme	carp	796:799	arg1	collagen					801:808	grass carp collagen	790:808	grass carp collagen (GCC) films	790:820	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	8	23	theme	GCC	1012:1014	arg1	ratio = 7:3					1023:1033	GCC:CS-LEO ratio = 7:3	1012:1033	GCC:CS-LEO ratio = 7:3	1012:1033	At GCC:CS-LEO ratio = 7:3, a maximum LEO release rate of 83.6 ± 9.7% was achieved over 15 days.					
32470587	3	24	dep	spherical	403:411	arg1	nanoparticles					384:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	24	dep	spherical	403:411	arg1	small					414:418	small	414:418	small	414:418	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	24	dep	spherical	403:411	arg1	stable					496:501	stable	496:501	stable	496:501	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	24	dep	spherical	403:411	arg1	spherical					403:411	spherical	403:411	spherical	403:411	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	25	theme	right	513:517	arg1	conditions					519:528	the right conditions	509:528	the right conditions	509:528	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	8	26	theme	%	1076:1076	arg1	rate					1058:1061	a maximum LEO release rate	1036:1061	a maximum LEO release rate of 83.6 ± 9.7%	1036:1076	At GCC:CS-LEO ratio = 7:3, a maximum LEO release rate of 83.6 ± 9.7% was achieved over 15 days.					
32470587	9	27	theme	pork	1227:1230	arg1	deterioration					1210:1222	the deterioration	1206:1222	the deterioration of pork	1206:1230	GCC/CS-LEO films can effectively inhibit lipid oxidation, prevent microbial proliferation, and delay the deterioration of pork at 4 °C for 21 days.					
32470587	7	28	theme	CS-LEO	995:1000	arg1	ratio					1002:1006	GCC:CS-LEO ratio	991:1006	GCC:CS-LEO ratio	991:1006	The LEO release rate increased with decreasing GCC:CS-LEO ratio.					
32470587	5	29	theme	X-ray	643:647	arg1	XPS					677:679	XPS	677:679	XPS	677:679	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	29	theme	X-ray	643:647	arg1	spectroscopy					663:674	X-ray photoelectron spectroscopy	643:674	X-ray photoelectron spectroscopy (XPS)	643:680	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	2	30	theme	CS	312:313	arg1	ratios					365:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	6	31	theme	higher	911:916	arg1	EB					939:940	EB	939:940	EB	939:940	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	31	theme	higher	911:916	arg1	elongation					918:927	higher elongation	911:927	higher elongation at break (EB)	911:941	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	8	32	theme	CS-LEO	1016:1021	arg1	ratio = 7:3					1023:1033	GCC:CS-LEO ratio = 7:3	1012:1033	GCC:CS-LEO ratio = 7:3	1012:1033	At GCC:CS-LEO ratio = 7:3, a maximum LEO release rate of 83.6 ± 9.7% was achieved over 15 days.					
32470587	6	33	theme	oxygen	851:856	arg1	OP					872:873	OP	872:873	OP	872:873	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	33	theme	oxygen	851:856	arg1	permeability					858:869	lower oxygen permeability	845:869	lower oxygen permeability (OP)	845:874	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	5	34	theme	photoelectron	649:661	arg1	XPS					677:679	XPS	677:679	XPS	677:679	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	34	theme	photoelectron	649:661	arg1	spectroscopy					663:674	X-ray photoelectron spectroscopy	643:674	X-ray photoelectron spectroscopy (XPS)	643:680	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	2	35	theme	different	302:310	arg1	ratios					365:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	5	36	theme	X-ray	753:757	arg1	diffraction					759:769	X-ray diffraction	753:769	X-ray diffraction (XRD)	753:775	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	36	theme	X-ray	753:757	arg1	XRD					772:774	XRD	772:774	XRD	772:774	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	0	37	theme	carp	42:45	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	0	37	theme	carp	42:45	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	3	38	theme	polydispersity	451:464	arg1	0.15					473:476	0.15	473:476	0.15	473:476	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	38	theme	polydispersity	451:464	arg1	index					466:470	a low polydispersity index	445:470	a low polydispersity index (0.15)	445:477	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	0	39	theme	grass	36:40	arg1	carp					42:45	grass carp	36:45	grass carp	36:45	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	5	40	theme	atomic	683:688	arg1	microscopy					696:705	atomic force microscopy	683:705	atomic force microscopy (AFM)	683:711	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	40	theme	atomic	683:688	arg1	AFM					708:710	AFM	708:710	AFM	708:710	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	3	41	theme	CS-LEO	377:382	arg1	nanoparticles					384:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles	373:396	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	41	theme	CS-LEO	377:382	arg1	small					414:418	small	414:418	small	414:418	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	41	theme	CS-LEO	377:382	arg1	stable					496:501	stable	496:501	stable	496:501	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	41	theme	CS-LEO	377:382	arg1	spherical					403:411	spherical	403:411	spherical	403:411	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	0	42	theme	oil	81:83	arg1	films					95:99	essential oil composite films	71:99	essential oil composite films	71:99	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	2	43	theme	chitosan	232:239	arg1	nanoparticles					250:262	various chitosan (CS)-LEO nanoparticles	224:262	various chitosan (CS)-LEO nanoparticles	224:262	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	3	44	theme	low	447:449	arg1	0.15					473:476	0.15	473:476	0.15	473:476	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	3	44	theme	low	447:449	arg1	index					466:470	a low polydispersity index	445:470	a low polydispersity index (0.15)	445:477	The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions.					
32470587	5	45	theme	electron	723:730	arg1	SEM					744:746	SEM	744:746	SEM	744:746	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	5	45	theme	electron	723:730	arg1	microscope					732:741	scanning electron microscope	714:741	scanning electron microscope (SEM)	714:747	The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM) and X-ray diffraction (XRD).					
32470587	6	46	theme	edible	823:828	arg1	GCC/CS-LEO					830:839	edible GCC/CS-LEO	823:839	edible GCC/CS-LEO	823:839	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	2	47	theme	CS	347:348	arg1	ratios					365:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	9	48	theme	microbial	1171:1179	arg1	proliferation					1181:1193	microbial proliferation	1171:1193	microbial proliferation	1171:1193	GCC/CS-LEO films can effectively inhibit lipid oxidation, prevent microbial proliferation, and delay the deterioration of pork at 4 °C for 21 days.					
32470587	2	49	theme	concentrations	328:341	arg1	ratios					365:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios	302:370	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	8	50	theme	LEO	1046:1048	arg1	rate					1058:1061	a maximum LEO release rate	1036:1061	a maximum LEO release rate of 83.6 ± 9.7%	1036:1076	At GCC:CS-LEO ratio = 7:3, a maximum LEO release rate of 83.6 ± 9.7% was achieved over 15 days.					
32470587	0	51	theme	composite	85:93	arg1	films					95:99	essential oil composite films	71:99	essential oil composite films	71:99	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	2	52	theme	-LEO	245:248	arg1	nanoparticles					250:262	various chitosan (CS)-LEO nanoparticles	224:262	various chitosan (CS)-LEO nanoparticles	224:262	Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios.					
32470587	8	53	theme	release	1050:1056	arg1	rate					1058:1061	a maximum LEO release rate	1036:1061	a maximum LEO release rate of 83.6 ± 9.7%	1036:1076	At GCC:CS-LEO ratio = 7:3, a maximum LEO release rate of 83.6 ± 9.7% was achieved over 15 days.					
32470587	0	54	theme	essential	71:79	arg1	films					95:99	essential oil composite films	71:99	essential oil composite films	71:99	Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.					
32470587	9	55	theme	GCC/CS-LEO	1105:1114	arg1	films					1116:1120	GCC/CS-LEO films	1105:1120	GCC/CS-LEO films	1105:1120	GCC/CS-LEO films can effectively inhibit lipid oxidation, prevent microbial proliferation, and delay the deterioration of pork at 4 °C for 21 days.					
32470587	6	56	contain	had	841:843	arg2	TS					902:903	TS	902:903	TS	902:903	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	56	contain	had	841:843	arg2	EB					939:940	EB	939:940	EB	939:940	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	56	contain	had	841:843	arg2	elongation					918:927	higher elongation	911:927	higher elongation at break (EB)	911:941	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	56	contain	had	841:843	arg2	OP					872:873	OP	872:873	OP	872:873	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	56	contain	had	841:843	arg1	GCC/CS-LEO					830:839	edible GCC/CS-LEO	823:839	edible GCC/CS-LEO	823:839	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	56	contain	had	841:843	arg2	strength					892:899	higher tensile strength	877:899	higher tensile strength (TS)	877:904	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	56	contain	had	841:843	arg2	permeability					858:869	lower oxygen permeability	845:869	lower oxygen permeability (OP)	845:874	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	9	57	theme	lipid	1146:1150	arg1	oxidation					1152:1160	lipid oxidation	1146:1160	lipid oxidation	1146:1160	GCC/CS-LEO films can effectively inhibit lipid oxidation, prevent microbial proliferation, and delay the deterioration of pork at 4 °C for 21 days.					
32470587	6	58	theme	tensile	884:890	arg1	strength					892:899	higher tensile strength	877:899	higher tensile strength (TS)	877:904	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
32470587	6	58	theme	tensile	884:890	arg1	TS					902:903	TS	902:903	TS	902:903	Relative to grass carp collagen (GCC) films, edible GCC/CS-LEO had lower oxygen permeability (OP), higher tensile strength (TS), and higher elongation at break (EB).					
31958554	0	0	theme	silver-loaded	71:83	arg1	dressing					101:108	silver-loaded composite wound dressing	71:108	silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property	71:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	8	1	theme	water	1220:1224	arg1	absorption					1226:1235	excellent water absorption	1210:1235	excellent water absorption	1210:1235	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	4	2	theme	sodium	731:736	arg1	alginate					738:745	sodium alginate	731:745	sodium alginate (SA)	731:750	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	4	2	theme	sodium	731:736	arg1	SA					748:749	SA	748:749	SA	748:749	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	1	3	theme	Wound	163:167	arg1	dressings					169:177	Wound dressings	163:177	Wound dressings	163:177	Wound dressings provide barrier protection during wound treatment while providing an environment suitable for wound healing.					
31958554	1	4	theme	wound	273:277	arg1	healing					279:285	wound healing	273:285	wound healing	273:285	Wound dressings provide barrier protection during wound treatment while providing an environment suitable for wound healing.					
31958554	8	5	theme	large	1277:1281	arg1	amount					1283:1288	a large amount	1275:1288	a large amount of wound exudate	1275:1305	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	8	5	theme	large	1277:1281	arg1	exudate					1299:1305	wound exudate	1293:1305	wound exudate	1293:1305	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	10	6	theme	silver-loaded	1478:1490	arg1	hydrogel					1492:1499	the silver-loaded hydrogel	1474:1499	the silver-loaded hydrogel	1474:1499	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	10	6	theme	silver-loaded	1478:1490	arg1	dressing					1519:1526	an ideal wound dressing	1504:1526	an ideal wound dressing	1504:1526	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	9	7	theme	mechanical	1394:1403	arg1	properties					1405:1414	good mechanical properties	1389:1414	good mechanical properties	1389:1414	The antibacterial hydrogel exhibited good mechanical properties, antibacterial activity and biocompatibility.					
31958554	0	8	theme	wound	95:99	arg1	dressing					101:108	silver-loaded composite wound dressing	71:108	silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property	71:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	4	9	theme	hydrogel	795:802	arg1	dressing					824:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	10	10	from	dressing	1519:1526	arg1	summary					1465:1471	summary	1465:1471	summary	1465:1471	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	0	11	theme	composite	85:93	arg1	dressing					101:108	silver-loaded composite wound dressing	71:108	silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property	71:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	3	12	theme	good	534:537	arg1	effect					553:558	good antibacterial effect	534:558	good antibacterial effect	534:558	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	8	13	theme	exudate	1299:1305	arg1	amount					1283:1288	a large amount	1275:1288	a large amount of wound exudate	1275:1305	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	8	13	theme	exudate	1299:1305	arg1	exudate					1299:1305	wound exudate	1293:1305	wound exudate	1293:1305	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	2	14	theme	wound	309:313	arg1	dressings					315:323	traditional wound dressings	297:323	traditional wound dressings	297:323	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	4	15	dep	in	708:709	arg1	situ					711:714	situ	711:714	situ	711:714	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	4	16	theme	wound	818:822	arg1	dressing					824:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	2	17	theme	traditional	297:307	arg1	dressings					315:323	traditional wound dressings	297:323	traditional wound dressings	297:323	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	2	18	theme	poor	406:409	arg1	haemostasis					411:421	poor haemostasis	406:421	poor haemostasis	406:421	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	6	19	theme	particle	955:962	arg1	small					972:976	small	972:976	small	972:976	The results show that SA can successfully reduce AgNPs, and the particle size is small and uniform, which meets the requirements of antibacterial material.					
31958554	6	19	theme	particle	955:962	arg1	size					964:967	the particle size	951:967	the particle size	951:967	The results show that SA can successfully reduce AgNPs, and the particle size is small and uniform, which meets the requirements of antibacterial material.					
31958554	4	20	theme	antibacterial	804:816	arg1	dressing					824:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	1	21	theme	barrier	187:193	arg1	protection					195:204	barrier protection	187:204	barrier protection	187:204	Wound dressings provide barrier protection during wound treatment while providing an environment suitable for wound healing.					
31958554	8	22	theme	uniform	1167:1173	arg1	pores					1175:1179	uniform pores	1167:1179	uniform pores inside the material	1167:1199	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	0	23	with	dressing	101:108	arg1	property					153:160	enhanced mechanical and antimicrobial property	115:160	enhanced mechanical and antimicrobial property	115:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	2	24	theme	such	345:348	arg1	effects					393:399	such as easily adhering to wounds, poor barrier effects	345:399	such as easily adhering to wounds, poor barrier effects	345:399	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	6	25	theme	material	1037:1044	arg1	requirements					1007:1018	the requirements	1003:1018	the requirements of antibacterial material	1003:1044	The results show that SA can successfully reduce AgNPs, and the particle size is small and uniform, which meets the requirements of antibacterial material.					
31958554	0	26	theme	In	0:1	arg1	reduction					8:16	In situ reduction	0:16	In situ reduction of silver	0:26	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	8	27	theme	excellent	1210:1218	arg1	absorption					1226:1235	excellent water absorption	1210:1235	excellent water absorption	1210:1235	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	4	28	theme	in	708:709	arg1	reduction					716:724	in situ reduction	708:724	in situ reduction with sodium alginate (SA)	708:750	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	5	29	theme	antibacterial	852:864	arg1	hydrogel					866:873	antibacterial hydrogel	852:873	antibacterial hydrogel	852:873	The properties of antibacterial hydrogel were evaluated.					
31958554	0	30	theme	mechanical	124:133	arg1	property					153:160	enhanced mechanical and antimicrobial property	115:160	enhanced mechanical and antimicrobial property	115:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	9	31	theme	antibacterial	1417:1429	arg1	activity					1431:1438	antibacterial activity	1417:1438	antibacterial activity	1417:1438	The antibacterial hydrogel exhibited good mechanical properties, antibacterial activity and biocompatibility.					
31958554	3	32	theme	antibacterial	539:551	arg1	effect					553:558	good antibacterial effect	534:558	good antibacterial effect	534:558	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	3	33	from	healing	579:585	arg1	view					590:593	view	590:593	view of the disadvantages of traditional wound dressings	590:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	1	34	theme	wound	213:217	arg1	treatment					219:227	wound treatment	213:227	wound treatment	213:227	Wound dressings provide barrier protection during wound treatment while providing an environment suitable for wound healing.					
31958554	8	35	theme	wound	1333:1337	arg1	environment					1339:1349	a moist wound environment	1325:1349	a moist wound environment	1325:1349	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	0	36	theme	enhanced	115:122	arg1	property					153:160	enhanced mechanical and antimicrobial property	115:160	enhanced mechanical and antimicrobial property	115:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	3	37	theme	dressings	637:645	arg1	disadvantages					602:614	the disadvantages	598:614	the disadvantages of traditional wound dressings	598:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	2	38	dep	adhering	360:367	arg1	poor					380:383	poor	380:383	poor	380:383	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	0	39	theme	antimicrobial	139:151	arg1	property					153:160	enhanced mechanical and antimicrobial property	115:160	enhanced mechanical and antimicrobial property	115:160	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	4	40	theme	silver-loaded	769:781	arg1	dressing					824:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	6	41	theme	antibacterial	1023:1035	arg1	material					1037:1044	antibacterial material	1023:1044	antibacterial material	1023:1044	The results show that SA can successfully reduce AgNPs, and the particle size is small and uniform, which meets the requirements of antibacterial material.					
31958554	0	42	theme	silver	21:26	arg1	reduction					8:16	In situ reduction	0:16	In situ reduction of silver	0:26	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	2	43	contain	have	325:328	arg2	effects					393:399	such as easily adhering to wounds, poor barrier effects	345:399	such as easily adhering to wounds, poor barrier effects	345:399	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	2	43	contain	have	325:328	arg2	disadvantages					330:342	disadvantages	330:342	disadvantages	330:342	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	2	43	contain	have	325:328	arg1	dressings					315:323	traditional wound dressings	297:323	traditional wound dressings	297:323	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	2	43	contain	have	325:328	arg2	haemostasis					411:421	poor haemostasis	406:421	poor haemostasis	406:421	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	3	44	theme	traditional	619:629	arg1	dressings					637:645	traditional wound dressings	619:645	traditional wound dressings	619:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	9	45	theme	good	1389:1392	arg1	properties					1405:1414	good mechanical properties	1389:1414	good mechanical properties	1389:1414	The antibacterial hydrogel exhibited good mechanical properties, antibacterial activity and biocompatibility.					
31958554	0	46	theme	sodium	45:50	arg1	alginate					52:59	sodium alginate	45:59	sodium alginate	45:59	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	4	47	with	reduction	716:724	arg1	alginate					738:745	sodium alginate	731:745	sodium alginate (SA)	731:750	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	4	47	with	reduction	716:724	arg1	SA					748:749	SA	748:749	SA	748:749	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	3	48	theme	wound	631:635	arg1	dressings					637:645	traditional wound dressings	619:645	traditional wound dressings	619:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	3	49	theme	wound	573:577	arg1	healing					579:585	wound healing	573:585	wound healing in view of the disadvantages of traditional wound dressings	573:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	8	50	theme	moist	1327:1331	arg1	environment					1339:1349	a moist wound environment	1325:1349	a moist wound environment	1325:1349	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	7	51	theme	stable	1109:1114	arg1	performance					1116:1126	stable performance	1109:1126	stable performance	1109:1126	The AgNPs are evenly distributed inside the hydrogel and have stable performance.					
31958554	10	52	theme	wound	1513:1517	arg1	hydrogel					1492:1499	the silver-loaded hydrogel	1474:1499	the silver-loaded hydrogel	1474:1499	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	10	52	theme	wound	1513:1517	arg1	dressing					1519:1526	an ideal wound dressing	1504:1526	an ideal wound dressing	1504:1526	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	4	53	theme	PVA/SA/CMCS	783:793	arg1	dressing					824:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing	767:831	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	2	54	theme	adhering	360:367	arg1	barrier					385:391	easily adhering to wounds, poor barrier	353:391	easily adhering to wounds, poor barrier	353:391	However, traditional wound dressings have disadvantages, such as easily adhering to wounds, poor barrier effects, and poor haemostasis.					
31958554	3	55	contain	has	530:532	arg1	dressing					485:492	a new wound dressing	473:492	a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings	473:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	3	55	contain	has	530:532	arg2	effect					553:558	good antibacterial effect	534:558	good antibacterial effect	534:558	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	10	56	theme	ideal	1507:1511	arg1	hydrogel					1492:1499	the silver-loaded hydrogel	1474:1499	the silver-loaded hydrogel	1474:1499	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	10	56	theme	ideal	1507:1511	arg1	dressing					1519:1526	an ideal wound dressing	1504:1526	an ideal wound dressing	1504:1526	In summary, the silver-loaded hydrogel is an ideal wound dressing.					
31958554	3	57	theme	disadvantages	602:614	arg1	view					590:593	view	590:593	view of the disadvantages of traditional wound dressings	590:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	4	58	theme	silver	663:668	arg1	AgNPs					685:689	AgNPs	685:689	AgNPs	685:689	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	4	58	theme	silver	663:668	arg1	nanoparticles					670:682	silver nanoparticles	663:682	silver nanoparticles (AgNPs)	663:690	In this paper, silver nanoparticles (AgNPs) were reduced by in situ reduction with sodium alginate (SA), and construct a silver-loaded PVA/SA/CMCS hydrogel antibacterial wound dressing.					
31958554	3	59	theme	new	475:477	arg1	dressing					485:492	a new wound dressing	473:492	a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings	473:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	8	60	theme	silver-loaded	1133:1145	arg1	hydrogel					1147:1154	The silver-loaded hydrogel	1129:1154	The silver-loaded hydrogel	1129:1154	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	7	61	contain	have	1104:1107	arg1	AgNPs					1051:1055	The AgNPs	1047:1055	The AgNPs	1047:1055	The AgNPs are evenly distributed inside the hydrogel and have stable performance.					
31958554	7	61	contain	have	1104:1107	arg2	performance					1116:1126	stable performance	1109:1126	stable performance	1109:1126	The AgNPs are evenly distributed inside the hydrogel and have stable performance.					
31958554	3	62	theme	wound	479:483	arg1	dressing					485:492	a new wound dressing	473:492	a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings	473:645	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	8	63	theme	wound	1293:1297	arg1	exudate					1299:1305	wound exudate	1293:1305	wound exudate	1293:1305	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	9	64	theme	antibacterial	1356:1368	arg1	hydrogel					1370:1377	The antibacterial hydrogel	1352:1377	The antibacterial hydrogel	1352:1377	The antibacterial hydrogel exhibited good mechanical properties, antibacterial activity and biocompatibility.					
31958554	8	65	theme	water	1241:1245	arg1	retention					1247:1255	water retention	1241:1255	water retention	1241:1255	The silver-loaded hydrogel was formed uniform pores inside the material, and had excellent water absorption and water retention, which can absorb a large amount of wound exudate while maintaining a moist wound environment.					
31958554	5	66	theme	hydrogel	866:873	arg1	properties					838:847	The properties	834:847	The properties of antibacterial hydrogel	834:873	The properties of antibacterial hydrogel were evaluated.					
31958554	3	67	theme	great	444:448	arg1	significance					450:461	great significance	444:461	great significance	444:461	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
31958554	1	68	theme	suitable	260:267	arg1	environment					248:258	an environment	245:258	an environment suitable for wound healing	245:285	Wound dressings provide barrier protection during wound treatment while providing an environment suitable for wound healing.					
31958554	0	69	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property.					
31958554	3	70	theme	further	514:520	arg1	injury					522:527	further injury	514:527	further injury	514:527	Therefore, it is of great significance to design a new wound dressing that does not cause further injury, has good antibacterial effect and promotes wound healing in view of the disadvantages of traditional wound dressings.					
34965723	2	0	from	procedures	215:224	arg1	approach					250:257	the current glycerol approach	229:257	the current glycerol approach	229:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	5	1	theme	intrinsic	801:809	arg1	icephilicity					811:822	intrinsic icephilicity	801:822	intrinsic icephilicity	801:822	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	7	2	gly	glycopeptide	1374:1385	arg2	glycopeptide					1374:1385	glycopeptide	1374:1385	glycopeptide	1374:1385	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	5	3	theme	post-thaw	949:957	arg1	cryosurvival					959:970	remarkable post-thaw cryosurvival	938:970	remarkable post-thaw cryosurvival of human erythrocytes	938:992	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	6	4	theme	Human	1065:1069	arg1	hematocrit					1090:1099	∼50% hematocrit	1085:1099	∼50% hematocrit	1085:1099	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	6	4	theme	Human	1065:1069	arg1	erythrocytes					1071:1082	Human erythrocytes	1065:1082	Human erythrocytes (∼50% hematocrit) in cryostores	1065:1114	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	7	5	theme	Raman	1259:1263	arg1	spectroscopy					1265:1276	Raman spectroscopy	1259:1276	Raman spectroscopy	1259:1276	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	3	6	with	cryopreservation	541:556	arg1	trehalose					563:571	trehalose	563:571	trehalose	563:571	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	7	7	theme	ice	1291:1293	arg1	shaping					1295:1301	dynamic ice shaping	1283:1301	dynamic ice shaping	1283:1301	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	5	8	from	variation	1021:1029	arg1	functions					1054:1062	functions	1054:1062	functions	1054:1062	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	5	8	from	variation	1021:1029	arg1	morphology					1039:1048	cell morphology	1034:1048	cell morphology	1034:1048	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	8	9	theme	glycerol-free	1665:1677	arg1	cryopreservation					1697:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	7	10	theme	calorimetry	1246:1256	arg1	Analyses					1212:1219	Analyses	1212:1219	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping	1212:1301	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	7	11	theme	bond	1428:1431	arg1	network					1433:1439	the hydrogen bond network	1415:1439	the hydrogen bond network in nonfrozen solutions	1415:1462	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	2	12	theme	biocompatible	316:328	arg1	cryoprotectants					330:344	biocompatible cryoprotectants	316:344	biocompatible cryoprotectants for optimal bioprotection of human erythrocytes	316:392	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	5	13	theme	slight	1014:1019	arg1	variation					1021:1029	a slight variation	1012:1029	a slight variation in cell morphology and functions	1012:1062	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	8	14	theme	trehalose-associated	1584:1603	arg1	"					1615:1615	a trehalose-associated "chaperone"	1582:1615	a trehalose-associated "chaperone"	1582:1615	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	8	14	theme	trehalose-associated	1584:1603	arg1	glycopeptide					1551:1562	the ACTIVE glycopeptide	1540:1562	the ACTIVE glycopeptide	1540:1562	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	7	15	theme	growth	1514:1519	arg1	inhibition					1478:1487	inhibition	1478:1487	inhibition of recrystallization and growth of ice	1478:1526	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	5	16	gly	glycopeptides	782:794	arg2	glycopeptides					782:794	The synthesized ACTIVE glycopeptides	759:794	The synthesized ACTIVE glycopeptides with intrinsic icephilicity	759:822	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	8	17	theme	erythrocyte	1685:1695	arg1	cryopreservation					1697:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	5	18	theme	synthesized	763:773	arg1	glycopeptides					782:794	The synthesized ACTIVE glycopeptides	759:794	The synthesized ACTIVE glycopeptides with intrinsic icephilicity	759:822	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	3	19	theme	membrane	522:529	arg1	surfaces					531:538	membrane surfaces	522:538	membrane surfaces	522:538	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	3	20	gly	glycopeptide	429:440	arg1	ε-poly					464:469	saccharide-grafted ε-poly	445:469	saccharide-grafted ε-poly(L-lysine)	445:479	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	3	20	gly	glycopeptide	429:440	arg1	L-lysine					471:478	L-lysine	471:478	L-lysine	471:478	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	3	20	gly	glycopeptide	429:440	arg2	glycopeptide					429:440	a novel ACTIVE glycopeptide	414:440	a novel ACTIVE glycopeptide	414:440	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	5	21	theme	membrane	846:853	arg1	surface					855:861	the membrane surface	842:861	the membrane surface of human erythrocytes	842:883	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	5	22	theme	cell	1034:1037	arg1	morphology					1039:1048	cell morphology	1034:1048	cell morphology	1034:1048	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	2	23	from	approach	250:257	arg1	light					184:188	light	184:188	light of cell-damaging washing procedures in the current glycerol approach	184:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	5	24	theme	ACTIVE	775:780	arg1	glycopeptides					782:794	The synthesized ACTIVE glycopeptides	759:794	The synthesized ACTIVE glycopeptides with intrinsic icephilicity	759:822	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	8	25	theme	chaperone	1606:1614	arg1	"					1615:1615	a trehalose-associated "chaperone"	1582:1615	a trehalose-associated "chaperone"	1582:1615	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	8	25	theme	chaperone	1606:1614	arg1	glycopeptide					1551:1562	the ACTIVE glycopeptide	1540:1562	the ACTIVE glycopeptide	1540:1562	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	8	26	theme	human	1679:1683	arg1	cryopreservation					1697:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	7	27	theme	ACTIVE	1367:1372	arg1	glycopeptide					1374:1385	glycopeptide	1374:1385	glycopeptide	1374:1385	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	1	28	theme	Ice	90:92	arg1	formation					94:102	Ice formation	90:102	Ice formation	90:102	Ice formation and recrystallization exert severe impairments to cellular cryopreservation.					
34965723	6	29	theme	high	1131:1134	arg1	cryosurvival					1136:1147	high cryosurvival	1131:1147	high cryosurvival	1131:1147	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	3	30	theme	ACTIVE	422:427	arg1	glycopeptide					429:440	a novel ACTIVE glycopeptide	414:440	a novel ACTIVE glycopeptide	414:440	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	7	31	theme	ice	1524:1526	arg1	recrystallization					1492:1508	recrystallization	1492:1508	recrystallization	1492:1508	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	7	31	theme	ice	1524:1526	arg1	growth					1514:1519	growth	1514:1519	growth	1514:1519	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	7	32	theme	spectroscopy	1265:1276	arg1	Analyses					1212:1219	Analyses	1212:1219	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping	1212:1301	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	3	33	theme	human	607:611	arg1	erythrocytes					613:624	human erythrocytes	607:624	human erythrocytes	607:624	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	6	34	theme	%	1088:1088	arg1	hematocrit					1090:1099	∼50% hematocrit	1085:1099	∼50% hematocrit	1085:1099	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	6	34	theme	%	1088:1088	arg1	erythrocytes					1071:1082	Human erythrocytes	1065:1082	Human erythrocytes (∼50% hematocrit) in cryostores	1065:1114	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	3	35	with	surfaces	531:538	arg1	trehalose					563:571	trehalose	563:571	trehalose	563:571	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	2	36	theme	optimal	350:356	arg1	bioprotection					358:370	optimal bioprotection	350:370	optimal bioprotection of human erythrocytes	350:392	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	37	from	network	1433:1439	arg1	solutions					1454:1462	nonfrozen solutions	1444:1462	nonfrozen solutions	1444:1462	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	6	38	from	erythrocytes	1071:1082	arg1	cryostores					1105:1114	cryostores	1105:1114	cryostores	1105:1114	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	0	39	theme	ACTIVE	25:30	arg1	Glycopeptides					32:44	Icephilic ACTIVE Glycopeptides	15:44	Icephilic ACTIVE Glycopeptides	15:44	Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.					
34965723	7	40	dep	glycopeptide	1374:1385	arg1	the					1363:1365	the	1363:1365	the	1363:1365	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	5	41	theme	erythrocytes	872:883	arg1	surface					855:861	the membrane surface	842:861	the membrane surface of human erythrocytes	842:883	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	0	42	theme	Icephilic	15:23	arg1	Glycopeptides					32:44	Icephilic ACTIVE Glycopeptides	15:44	Icephilic ACTIVE Glycopeptides	15:44	Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.					
34965723	8	43	from	candidate	1652:1660	arg1	cryopreservation					1697:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	glycerol-free human erythrocyte cryopreservation	1665:1712	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	2	44	theme	procedures	215:224	arg1	light					184:188	light	184:188	light of cell-damaging washing procedures in the current glycerol approach	184:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	2	45	theme	cryoprotectants	330:344	arg1	development					301:311	the development	297:311	the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes	297:392	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	46	theme	shaping	1295:1301	arg1	Analyses					1212:1219	Analyses	1212:1219	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping	1212:1301	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	3	47	theme	ε-poly	464:469	arg1	glycopeptide					429:440	a novel ACTIVE glycopeptide	414:440	a novel ACTIVE glycopeptide	414:440	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	5	48	theme	human	975:979	arg1	erythrocytes					981:992	human erythrocytes	975:992	human erythrocytes	975:992	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	7	49	theme	differential	1224:1235	arg1	calorimetry					1246:1256	differential scanning calorimetry	1224:1256	differential scanning calorimetry	1224:1256	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	2	50	theme	washing	207:213	arg1	procedures					215:224	cell-damaging washing procedures	193:224	cell-damaging washing procedures in the current glycerol approach	193:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	51	theme	nonfrozen	1444:1452	arg1	solutions					1454:1462	nonfrozen solutions	1444:1462	nonfrozen solutions	1444:1462	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	3	52	with	icephilicity	578:589	arg1	trehalose					563:571	trehalose	563:571	trehalose	563:571	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	0	53	theme	Glycopeptides	32:44	arg1	Development					0:10	Development	0:10	Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.	0:88	Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.					
34965723	2	54	theme	cell-damaging	193:205	arg1	procedures					215:224	cell-damaging washing procedures	193:224	cell-damaging washing procedures in the current glycerol approach	193:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	2	55	theme	many	260:263	arg1	researches					265:274	many researches	260:274	many researches	260:274	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	56	theme	scanning	1237:1244	arg1	calorimetry					1246:1256	differential scanning calorimetry	1224:1256	differential scanning calorimetry	1224:1256	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	2	57	from	light	184:188	arg1	approach					250:257	the current glycerol approach	229:257	the current glycerol approach	229:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	8	58	theme	new	1630:1632	arg1	way					1634:1636	a new way	1628:1636	a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation	1628:1712	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	8	59	theme	ACTIVE	1544:1549	arg1	"					1615:1615	a trehalose-associated "chaperone"	1582:1615	a trehalose-associated "chaperone"	1582:1615	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	8	59	theme	ACTIVE	1544:1549	arg1	glycopeptide					1551:1562	the ACTIVE glycopeptide	1540:1562	the ACTIVE glycopeptide	1540:1562	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	1	60	theme	severe	132:137	arg1	impairments					139:149	severe impairments	132:149	severe impairments	132:149	Ice formation and recrystallization exert severe impairments to cellular cryopreservation.					
34965723	3	61	theme	saccharide-grafted	445:462	arg1	L-lysine					471:478	L-lysine	471:478	L-lysine	471:478	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	3	61	theme	saccharide-grafted	445:462	arg1	ε-poly					464:469	saccharide-grafted ε-poly	445:469	saccharide-grafted ε-poly(L-lysine)	445:479	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	2	62	theme	glycerol	241:248	arg1	approach					250:257	the current glycerol approach	229:257	the current glycerol approach	229:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	63	theme	cryopreservation	1323:1338	arg1	protocol					1340:1347	this cryopreservation protocol	1318:1347	this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose	1318:1399	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	2	64	theme	erythrocytes	381:392	arg1	bioprotection					358:370	optimal bioprotection	350:370	optimal bioprotection of human erythrocytes	350:392	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	65	theme	recrystallization	1492:1508	arg1	inhibition					1478:1487	inhibition	1478:1487	inhibition of recrystallization and growth of ice	1478:1526	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	2	66	theme	current	233:239	arg1	approach					250:257	the current glycerol approach	229:257	the current glycerol approach	229:257	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	7	67	theme	hydrogen	1419:1426	arg1	network					1433:1439	the hydrogen bond network	1415:1439	the hydrogen bond network in nonfrozen solutions	1415:1462	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
34965723	6	68	theme	∼50	1085:1087	arg1	%					1088:1088	%	1088:1088	%	1088:1088	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	2	69	theme	human	375:379	arg1	erythrocytes					381:392	human erythrocytes	375:392	human erythrocytes	375:392	In light of cell-damaging washing procedures in the current glycerol approach, many researches have been devoted to the development of biocompatible cryoprotectants for optimal bioprotection of human erythrocytes.					
34965723	4	70	theme	reductive	642:650	arg1	amination					652:660	Borch reductive amination	636:660	Borch reductive amination	636:660	Then, by Borch reductive amination or amidation, glucose, lactose, maltose, maltotriose, or trehalose was tethered to ε-polylysine.					
34965723	5	71	theme	erythrocytes	981:992	arg1	cryosurvival					959:970	remarkable post-thaw cryosurvival	938:970	remarkable post-thaw cryosurvival of human erythrocytes	938:992	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	5	72	theme	human	866:870	arg1	erythrocytes					872:883	human erythrocytes	866:883	human erythrocytes	866:883	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	1	73	theme	cellular	154:161	arg1	cryopreservation					163:178	cellular cryopreservation	154:178	cellular cryopreservation	154:178	Ice formation and recrystallization exert severe impairments to cellular cryopreservation.					
34965723	4	74	theme	Borch	636:640	arg1	amination					652:660	Borch reductive amination	636:660	Borch reductive amination	636:660	Then, by Borch reductive amination or amidation, glucose, lactose, maltose, maltotriose, or trehalose was tethered to ε-polylysine.					
34965723	0	75	theme	Erythrocytes	76:87	arg1	Cryopreservation					50:65	Cryopreservation	50:65	Cryopreservation of Human Erythrocytes	50:87	Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.					
34965723	8	76	gly	glycopeptide	1551:1562	arg2	glycopeptide					1551:1562	the ACTIVE glycopeptide	1540:1562	the ACTIVE glycopeptide	1540:1562	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	8	76	gly	glycopeptide	1551:1562	arg2	"					1615:1615	a trehalose-associated "chaperone"	1582:1615	a trehalose-associated "chaperone"	1582:1615	Therefore, the ACTIVE glycopeptide can be applied as a trehalose-associated "chaperone", providing a new way to serve as a candidate in glycerol-free human erythrocyte cryopreservation.					
34965723	5	77	with	glycopeptides	782:794	arg1	icephilicity					811:822	intrinsic icephilicity	801:822	intrinsic icephilicity	801:822	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	5	78	theme	remarkable	938:947	arg1	cryosurvival					959:970	remarkable post-thaw cryosurvival	938:970	remarkable post-thaw cryosurvival of human erythrocytes	938:992	The synthesized ACTIVE glycopeptides with intrinsic icephilicity could localize on the membrane surface of human erythrocytes and improve cryopreservation with trehalose, so that remarkable post-thaw cryosurvival of human erythrocytes was achieved with a slight variation in cell morphology and functions.					
34965723	0	79	theme	Human	70:74	arg1	Erythrocytes					76:87	Human Erythrocytes	70:87	Human Erythrocytes	70:87	Development of Icephilic ACTIVE Glycopeptides for Cryopreservation of Human Erythrocytes.					
34965723	6	80	theme	liquid	1182:1187	arg1	nitrogen					1189:1196	liquid nitrogen	1182:1196	liquid nitrogen	1182:1196	Human erythrocytes (∼50% hematocrit) in cryostores could maintain high cryosurvival above 74%, even after plunged in liquid nitrogen for 6 months.					
34965723	3	81	theme	novel	416:420	arg1	glycopeptide					429:440	a novel ACTIVE glycopeptide	414:440	a novel ACTIVE glycopeptide	414:440	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	3	82	theme	erythrocytes	613:624	arg1	validity					595:602	validity	595:602	validity of human erythrocytes	595:624	Herein, we develop a novel ACTIVE glycopeptide of saccharide-grafted ε-poly(L-lysine), that can be credited with adsorption on membrane surfaces, cryopreservation with trehalose, and icephilicity for validity of human erythrocytes.					
34965723	7	83	theme	dynamic	1283:1289	arg1	shaping					1295:1301	dynamic ice shaping	1283:1301	dynamic ice shaping	1283:1301	Analyses of differential scanning calorimetry, Raman spectroscopy, and dynamic ice shaping suggested that this cryopreservation protocol combined with the ACTIVE glycopeptide and trehalose could enhance the hydrogen bond network in nonfrozen solutions, resulting in inhibition of recrystallization and growth of ice.					
32561280	0	0	theme	pulp	92:95	arg1	cells					102:106	dental pulp stem cells	85:106	dental pulp stem cells scaffold for bone tissue engineering	85:143	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	7	1	theme	hDPSCs	1628:1633	arg1	transplantation					1609:1623	the transplantation	1605:1623	the transplantation of hDPSCs	1605:1633	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	6	2	theme	inducer	1422:1428	arg1	absorption					1397:1406	the absorption	1393:1406	the absorption of osteogenic inducer	1393:1428	It seems to promote the absorption of osteogenic inducer too.					
32561280	1	3	theme	copolymer/graphene	258:275	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	3	theme	copolymer/graphene	258:275	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	0	4	theme	dental	85:90	arg1	cells					102:106	dental pulp stem cells	85:106	dental pulp stem cells scaffold for bone tissue engineering	85:143	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	3	5	theme	formulated	783:792	arg1	hydrogels					794:802	The formulated hydrogels	779:802	The formulated hydrogels	779:802	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	4	6	theme	minerals	1036:1043	arg1	activity					1053:1060	the activity	1049:1060	the activity of alkaline phosphatase (ALP)	1049:1090	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	6	theme	minerals	1036:1043	arg1	deposition					1022:1031	the deposition	1018:1031	the deposition of minerals	1018:1043	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	1	7	theme	oxide	277:281	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	7	theme	oxide	277:281	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	0	8	theme	cells	102:106	arg1	scaffold					108:115	dental pulp stem cells scaffold	85:115	dental pulp stem cells scaffold for bone tissue engineering	85:143	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	1	9	dep	composite	288:296	arg1	GO					284:285	GO	284:285	GO	284:285	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	7	10	from	scaffold	1565:1572	arg1	engineering					1589:1599	bone tissue engineering	1577:1599	bone tissue engineering for the transplantation of hDPSCs	1577:1633	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	4	11	theme	CS	1183:1184	arg1	GO					1176:1177	GO	1176:1177	GO	1176:1177	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	11	theme	CS	1183:1184	arg1	groups					1166:1171	amine-containing functional groups	1138:1171	amine-containing functional groups	1138:1171	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	11	theme	CS	1183:1184	arg1	CS					1183:1184	CS	1183:1184	CS	1183:1184	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	11	theme	CS	1183:1184	arg1	oxygen					1127:1132	oxygen	1127:1132	oxygen	1127:1132	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	2	12	theme	hydrogels	768:776	arg1	preparation					749:759	the preparation	745:759	the preparation of the hydrogels	745:776	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	0	13	theme	stem	97:100	arg1	cells					102:106	dental pulp stem cells	85:106	dental pulp stem cells scaffold for bone tissue engineering	85:143	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	5	14	theme	normal	1344:1349	arg1	media					1366:1370	both the normal and osteogenic media	1335:1370	media	1366:1370	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	1	15	theme	natural	346:352	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	15	theme	natural	346:352	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	16	theme	human	454:458	arg1	hDPSCs					484:489	hDPSCs	484:489	hDPSCs	484:489	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	16	theme	human	454:458	arg1	cells					477:481	the human dental pulp stem cells	450:481	the human dental pulp stem cells (hDPSCs)	450:490	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	3	17	theme	compressive	870:880	arg1	strength					882:889	compressive strength	870:889	compressive strength	870:889	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	1	18	theme	thermosensitive	166:180	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	18	theme	thermosensitive	166:180	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	19	theme	dental	460:465	arg1	hDPSCs					484:489	hDPSCs	484:489	hDPSCs	484:489	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	19	theme	dental	460:465	arg1	cells					477:481	the human dental pulp stem cells	450:481	the human dental pulp stem cells (hDPSCs)	450:490	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	5	20	theme	osteogenic	1355:1364	arg1	media					1366:1370	both the normal and osteogenic media	1335:1370	media	1366:1370	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	3	21	theme	rheological	848:858	arg1	behavior					860:867	rheological behavior	848:867	rheological behavior	848:867	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	1	22	theme	injectable	182:191	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	22	theme	injectable	182:191	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	0	23	theme	thermosensitive	11:25	arg1	hydrogel					34:41	Injectable thermosensitive hybrid hydrogel	0:41	Injectable thermosensitive hybrid hydrogel	0:41	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	5	24	theme	Runt-related	1255:1266	arg1	factor					1282:1287	the Runt-related transcription factor 2	1251:1289	the Runt-related transcription factor 2	1251:1289	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	2	25	theme	NIPAAm	624:629	arg1	copolymerization					579:594	free-radical copolymerization	566:594	free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG)	566:708	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	1	26	theme	pulp	467:470	arg1	hDPSCs					484:489	hDPSCs	484:489	hDPSCs	484:489	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	26	theme	pulp	467:470	arg1	cells					477:481	the human dental pulp stem cells	450:481	the human dental pulp stem cells (hDPSCs)	450:490	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	6	27	theme	osteogenic	1411:1420	arg1	inducer					1422:1428	osteogenic inducer	1411:1428	osteogenic inducer	1411:1428	It seems to promote the absorption of osteogenic inducer too.					
32561280	1	28	theme	hydrogel	193:200	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	28	theme	hydrogel	193:200	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	0	29	theme	Injectable	0:9	arg1	hydrogel					34:41	Injectable thermosensitive hybrid hydrogel	0:41	Injectable thermosensitive hybrid hydrogel	0:41	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	1	30	theme	stem	472:475	arg1	hDPSCs					484:489	hDPSCs	484:489	hDPSCs	484:489	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	30	theme	stem	472:475	arg1	cells					477:481	the human dental pulp stem cells	450:481	the human dental pulp stem cells (hDPSCs)	450:490	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	3	31	theme	swelling	892:899	arg1	property					901:908	swelling property	892:908	swelling property	892:908	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	1	32	theme	containing	202:211	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	32	theme	containing	202:211	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	7	33	theme	tissue	1582:1587	arg1	engineering					1589:1599	bone tissue engineering	1577:1599	bone tissue engineering for the transplantation of hDPSCs	1577:1633	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	0	34	theme	tissue	126:131	arg1	engineering					133:143	bone tissue engineering	121:143	bone tissue engineering	121:143	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	1	35	theme	cells	477:481	arg1	differentiation					431:445	differentiation	431:445	differentiation	431:445	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	35	theme	cells	477:481	arg1	proliferation					413:425	the proliferation	409:425	the proliferation	409:425	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	2	36	theme	itaconic	633:640	arg1	NIPAAm					624:629	NIPAAm	624:629	NIPAAm	624:629	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	36	theme	itaconic	633:640	arg1	IA					648:649	IA	648:649	IA	648:649	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	36	theme	itaconic	633:640	arg1	acid					642:645	itaconic acid	633:645	itaconic acid (IA)	633:650	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	4	37	theme	alkaline	1065:1072	arg1	ALP					1087:1089	ALP	1087:1089	ALP	1087:1089	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	37	theme	alkaline	1065:1072	arg1	phosphatase					1074:1084	alkaline phosphatase	1065:1084	alkaline phosphatase (ALP)	1065:1090	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	1	38	theme	poly	213:216	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	38	theme	poly	213:216	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	0	39	theme	hybrid	27:32	arg1	hydrogel					34:41	Injectable thermosensitive hybrid hydrogel	0:41	Injectable thermosensitive hybrid hydrogel	0:41	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	7	40	theme	bone	1577:1580	arg1	engineering					1589:1599	bone tissue engineering	1577:1599	bone tissue engineering for the transplantation of hDPSCs	1577:1633	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	0	41	theme	bone	121:124	arg1	engineering					133:143	bone tissue engineering	121:143	bone tissue engineering	121:143	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	1	42	theme	physical	370:377	arg1	crosslinking					392:403	physical and chemical crosslinking	370:403	physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	370:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	4	43	dep	oxygen	1127:1132	arg1	the					1123:1125	the	1123:1125	the	1123:1125	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	5	44	theme	factor	1282:1287	arg1	expression					1237:1246	the expression	1233:1246	the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media	1233:1370	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	4	45	theme	phosphatase	1074:1084	arg1	activity					1053:1060	the activity	1049:1060	the activity of alkaline phosphatase (ALP)	1049:1090	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	45	theme	phosphatase	1074:1084	arg1	deposition					1022:1031	the deposition	1018:1031	the deposition of minerals	1018:1043	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	0	46	theme	graphene	54:61	arg1	oxide					63:67	graphene oxide	54:67	graphene oxide	54:67	Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.					
32561280	1	47	theme	different	303:311	arg1	ratio					318:322	different feed ratio	303:322	different feed ratio to chitosan (CS)	303:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	2	48	theme	copolymer/GO	524:535	arg1	composite					537:545	The PNIPAAm copolymer/GO composite	512:545	The PNIPAAm copolymer/GO composite	512:545	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	5	49	dep	media	1366:1370	arg1	both					1335:1338	both	1335:1338	both	1335:1338	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	2	50	theme	GO	729:730	arg1	presence					717:724	the presence	713:724	the presence of GO	713:730	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	1	51	theme	N-isopropylacrylamide	219:239	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	51	theme	N-isopropylacrylamide	219:239	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	52	theme	feed	313:316	arg1	ratio					318:322	different feed ratio	303:322	different feed ratio to chitosan (CS)	303:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	2	53	theme	PNIPAAm	516:522	arg1	composite					537:545	The PNIPAAm copolymer/GO composite	512:545	The PNIPAAm copolymer/GO composite	512:545	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	1	54	theme	chemical	383:390	arg1	crosslinking					392:403	physical and chemical crosslinking	370:403	physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	370:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	4	55	theme	GO	1176:1177	arg1	GO					1176:1177	GO	1176:1177	GO	1176:1177	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	55	theme	GO	1176:1177	arg1	groups					1166:1171	amine-containing functional groups	1138:1171	amine-containing functional groups	1138:1171	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	55	theme	GO	1176:1177	arg1	CS					1183:1184	CS	1183:1184	CS	1183:1184	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	55	theme	GO	1176:1177	arg1	oxygen					1127:1132	oxygen	1127:1132	oxygen	1127:1132	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	7	56	theme	constructing	1552:1563	arg1	scaffold					1565:1572	a constructing scaffold	1550:1572	a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs	1550:1633	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	7	56	theme	constructing	1552:1563	arg1	it					1532:1533	it	1532:1533	it	1532:1533	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	2	57	used	used	736:739	arg2	composite					537:545	The PNIPAAm copolymer/GO composite	512:545	The PNIPAAm copolymer/GO composite	512:545	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	58	theme	ethylene	687:694	arg1	poly					682:685	maleic anhydride-modified poly	656:685	maleic anhydride-modified poly(ethylene glycol) (PEG)	656:708	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	58	theme	ethylene	687:694	arg1	glycol					696:701	ethylene glycol	687:701	ethylene glycol	687:701	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	59	theme	free-radical	566:577	arg1	copolymerization					579:594	free-radical copolymerization	566:594	free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG)	566:708	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	7	60	theme	engineered	1462:1471	arg1	hydrogel					1473:1480	the engineered hydrogel	1458:1480	the engineered hydrogel	1458:1480	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	4	61	theme	functional	1155:1164	arg1	GO					1176:1177	GO	1176:1177	GO	1176:1177	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	61	theme	functional	1155:1164	arg1	groups					1166:1171	amine-containing functional groups	1138:1171	amine-containing functional groups	1138:1171	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	61	theme	functional	1155:1164	arg1	CS					1183:1184	CS	1183:1184	CS	1183:1184	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	5	62	theme	transcription	1268:1280	arg1	factor					1282:1287	the Runt-related transcription factor 2	1251:1289	the Runt-related transcription factor 2	1251:1289	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	7	63	theme	osteogenic	1499:1508	arg1	potential					1510:1518	the osteogenic potential	1495:1518	the osteogenic potential	1495:1518	Based on our findings, the engineered hydrogel demonstrated the osteogenic potential, upon which it is proposed as a constructing scaffold in bone tissue engineering for the transplantation of hDPSCs.					
32561280	1	64	with	composite	288:296	arg1	ratio					318:322	different feed ratio	303:322	different feed ratio to chitosan (CS)	303:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	2	65	theme	anhydride-modified	663:680	arg1	NIPAAm					624:629	NIPAAm	624:629	NIPAAm	624:629	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	65	theme	anhydride-modified	663:680	arg1	poly					682:685	maleic anhydride-modified poly	656:685	maleic anhydride-modified poly(ethylene glycol) (PEG)	656:708	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	65	theme	anhydride-modified	663:680	arg1	glycol					696:701	ethylene glycol	687:701	ethylene glycol	687:701	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	65	theme	anhydride-modified	663:680	arg1	PEG					705:707	PEG	705:707	PEG	705:707	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	3	66	theme	in	911:912	arg1	degradation					920:930	in vitro degradation	911:930	in vitro degradation	911:930	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	4	67	theme	amine-containing	1138:1153	arg1	GO					1176:1177	GO	1176:1177	GO	1176:1177	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	67	theme	amine-containing	1138:1153	arg1	groups					1166:1171	amine-containing functional groups	1138:1171	amine-containing functional groups	1138:1171	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	67	theme	amine-containing	1138:1153	arg1	CS					1183:1184	CS	1183:1184	CS	1183:1184	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	68	theme	attributable	1107:1118	arg1	part					1102:1105	large part	1096:1105	large part attributable to the oxygen and amine-containing functional groups of GO and CS	1096:1184	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	4	69	theme	large	1096:1100	arg1	part					1102:1105	large part	1096:1105	large part attributable to the oxygen and amine-containing functional groups of GO and CS	1096:1184	The hydrogel could enhance the deposition of minerals and the activity of alkaline phosphatase (ALP), in large part attributable to the oxygen and amine-containing functional groups of GO and CS.					
32561280	1	70	theme	PNIPAAm	243:249	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	70	theme	PNIPAAm	243:249	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	3	71	theme	porous	827:832	arg1	architecture					834:845	the porous architecture	823:845	the porous architecture	823:845	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	5	72	theme	engineered	1191:1200	arg1	hydrogel					1202:1209	The engineered hydrogel	1187:1209	The engineered hydrogel	1187:1209	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	5	73	theme	osteocalcin	1295:1305	arg1	expression					1237:1246	the expression	1233:1246	the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media	1233:1370	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	5	74	from	expression	1237:1246	arg1	hDPSCs					1314:1319	the hDPSCs	1310:1319	the hDPSCs cultivated in both the normal and osteogenic media	1310:1370	The engineered hydrogel could also upregulate the expression of the Runt-related transcription factor 2 and osteocalcin in the hDPSCs cultivated in both the normal and osteogenic media.					
32561280	2	75	theme	maleic	656:661	arg1	NIPAAm					624:629	NIPAAm	624:629	NIPAAm	624:629	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	75	theme	maleic	656:661	arg1	poly					682:685	maleic anhydride-modified poly	656:685	maleic anhydride-modified poly(ethylene glycol) (PEG)	656:708	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	75	theme	maleic	656:661	arg1	glycol					696:701	ethylene glycol	687:701	ethylene glycol	687:701	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	2	75	theme	maleic	656:661	arg1	PEG					705:707	PEG	705:707	PEG	705:707	The PNIPAAm copolymer/GO composite was synthesized by free-radical copolymerization of (N-isopropylacrylamide) (NIPAAm), itaconic acid (IA) and maleic anhydride-modified poly(ethylene glycol) (PEG) in the presence of GO and used for the preparation of the hydrogels.					
32561280	3	76	dep	in	911:912	arg1	vitro					914:918	vitro	914:918	vitro	914:918	The formulated hydrogels were evaluated for the porous architecture, rheological behavior, compressive strength, swelling property, in vitro degradation, hemocompatibility, biocompatibility, and differentiation.					
32561280	1	77	theme	-based	251:256	arg1	polymer					354:360	a natural polymer	344:360	a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts	344:509	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
32561280	1	77	theme	-based	251:256	arg1	composite					288:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite	166:296	thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS)	166:339	Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human dental pulp stem cells (hDPSCs) to the osteoblasts.					
34097961	5	0	theme	study	787:791	arg1	objective					769:777	The objective	765:777	The objective of this study	765:791	The objective of this study was to evaluate the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms.					
34097961	4	1	theme	-β-Galp	666:672	arg1	1 → residues					674:685	→6)-β-Galp(1 → residues	663:685	→6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3	663:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	1	2	theme	chronic	242:248	arg1	effects					203:209	their beneficial effects	186:209	their beneficial effects on diabetes	186:221	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	2	theme	chronic	242:248	arg1	diseases					260:267	NAFLD and related chronic metabolic diseases	224:267	NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD)	224:298	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	0	3	theme	anti-obesity	81:92	arg1	effects					94:100	anti-obesity effects	81:100	anti-obesity effects	81:100	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	4	4	with	1 → residues	674:685	arg1	branches					692:699	branches	692:699	branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3	692:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	5	5	theme	figure	849:854	arg1	effect					829:834	the anti-obesogenic effect	809:834	the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms	809:884	The objective of this study was to evaluate the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms.					
34097961	3	6	theme	homogeneous	480:490	arg1	LBP-W					508:512	LBP-W	508:512	LBP-W	508:512	Herein, a homogeneous polysaccharide (LBP-W) was isolated and purified from crude LBPs.					
34097961	3	6	theme	homogeneous	480:490	arg1	polysaccharide					492:505	a homogeneous polysaccharide	478:505	a homogeneous polysaccharide (LBP-W)	478:513	Herein, a homogeneous polysaccharide (LBP-W) was isolated and purified from crude LBPs.					
34097961	1	7	theme	metabolic	250:258	arg1	effects					203:209	their beneficial effects	186:209	their beneficial effects on diabetes	186:221	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	7	theme	metabolic	250:258	arg1	diseases					260:267	NAFLD and related chronic metabolic diseases	224:267	NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD)	224:298	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	4	8	contain	contained	607:615	arg1	LBP-W					601:605	LBP-W	601:605	LBP-W	601:605	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	4	8	contain	contained	607:615	arg2	chain					624:628	a main chain	617:628	a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3	617:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	7	9	theme	flora	1093:1097	arg1	diversity					1069:1077	community diversity	1059:1077	community diversity of intestinal flora	1059:1097	Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera.					
34097961	1	10	dep	Lycium	133:138	arg1	barbarum					140:147	barbarum	140:147	barbarum	140:147	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	6	11	theme	LBP-W	927:931	arg1	supplements					933:943	LBP-W supplements	927:943	LBP-W supplements	927:943	In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly.					
34097961	4	12	theme	β-Galp	721:726	arg1	residues					739:746	α-Araf, β-Galp and α-Rhap residues	713:746	α-Araf, β-Galp and α-Rhap residues at position C-3	713:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	4	13	theme	Structure	558:566	arg1	characterizations					568:584	Structure characterizations	558:584	Structure characterizations	558:584	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	0	14	theme	gut	117:119	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	8	15	theme	flora	1250:1254	arg1	metabolite					1221:1230	a metabolite	1219:1230	a metabolite of the intestinal flora	1219:1254	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	8	15	theme	flora	1250:1254	arg1	acids					1204:1208	short-chain fatty acids	1186:1208	short-chain fatty acids (SCFAs)	1186:1216	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	8	16	theme	intestinal	1239:1248	arg1	flora					1250:1254	the intestinal flora	1235:1254	the intestinal flora	1235:1254	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	9	17	theme	prebiotic	1381:1389	arg1	polysaccharide					1321:1334	the homogeneous polysaccharide	1305:1334	the homogeneous polysaccharide purified from L. barbarum	1305:1360	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	9	17	theme	prebiotic	1381:1389	arg1	agent					1391:1395	a prebiotic agent	1379:1395	a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs	1379:1492	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	8	18	theme	fatty	1198:1202	arg1	metabolite					1221:1230	a metabolite	1219:1230	a metabolite of the intestinal flora	1219:1254	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	8	18	theme	fatty	1198:1202	arg1	SCFAs					1211:1215	SCFAs	1211:1215	SCFAs	1211:1215	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	8	18	theme	fatty	1198:1202	arg1	acids					1204:1208	short-chain fatty acids	1186:1208	short-chain fatty acids (SCFAs)	1186:1216	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	0	19	from	barbarum	41:48	arg1	polysaccharide					14:27	A homogeneous polysaccharide	0:27	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.	0:131	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	6	20	theme	mice	971:974	arg1	obesity					976:982	HFD-induced mice obesity	959:982	HFD-induced mice obesity	959:982	In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly.					
34097961	4	21	theme	α-Rhap	732:737	arg1	residues					739:746	α-Araf, β-Galp and α-Rhap residues	713:746	α-Araf, β-Galp and α-Rhap residues at position C-3	713:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	5	22	theme	anti-obesogenic	813:827	arg1	effect					829:834	the anti-obesogenic effect	809:834	the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms	809:884	The objective of this study was to evaluate the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms.					
34097961	0	23	theme	homogeneous	2:12	arg1	polysaccharide					14:27	A homogeneous polysaccharide	0:27	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.	0:131	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	9	24	used	used	1371:1374	arg2	agent					1391:1395	a prebiotic agent	1379:1395	a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs	1379:1492	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	9	24	used	used	1371:1374	arg2	polysaccharide					1321:1334	the homogeneous polysaccharide	1305:1334	the homogeneous polysaccharide purified from L. barbarum	1305:1360	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	7	25	theme	community	1059:1067	arg1	diversity					1069:1077	community diversity	1059:1077	community diversity of intestinal flora	1059:1097	Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera.					
34097961	2	26	theme	crude	361:365	arg1	polysaccharides					367:381	the whole crude polysaccharides	351:381	the whole crude polysaccharides	351:381	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	3	27	theme	crude	546:550	arg1	LBPs					552:555	crude LBPs	546:555	crude LBPs	546:555	Herein, a homogeneous polysaccharide (LBP-W) was isolated and purified from crude LBPs.					
34097961	2	28	theme	whole	355:359	arg1	polysaccharides					367:381	the whole crude polysaccharides	351:381	the whole crude polysaccharides	351:381	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	2	29	theme	LBPs	418:421	arg1	ingredient					404:413	the specific active ingredient	384:413	the specific active ingredient of LBPs and its bioactivity	384:441	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	1	30	theme	high-fat	280:287	arg1	diet					289:292	high-fat diet	280:292	high-fat diet (HFD)	280:298	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	30	theme	high-fat	280:287	arg1	HFD					295:297	HFD	295:297	HFD	295:297	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	8	31	theme	short-chain	1186:1196	arg1	metabolite					1221:1230	a metabolite	1219:1230	a metabolite of the intestinal flora	1219:1254	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	8	31	theme	short-chain	1186:1196	arg1	SCFAs					1211:1215	SCFAs	1211:1215	SCFAs	1211:1215	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	8	31	theme	short-chain	1186:1196	arg1	acids					1204:1208	short-chain fatty acids	1186:1208	short-chain fatty acids (SCFAs)	1186:1216	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	1	32	theme	beneficial	192:201	arg1	effects					203:209	their beneficial effects	186:209	their beneficial effects on diabetes	186:221	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	32	theme	beneficial	192:201	arg1	diseases					260:267	NAFLD and related chronic metabolic diseases	224:267	NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD)	224:298	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	4	33	from	C-3	760:762	arg1	residues					739:746	α-Araf, β-Galp and α-Rhap residues	713:746	α-Araf, β-Galp and α-Rhap residues at position C-3	713:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	9	34	theme	flora	1460:1464	arg1	metabolism					1474:1483	the metabolism	1470:1483	the metabolism of SCFAs	1470:1492	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	9	34	theme	flora	1460:1464	arg1	composition					1434:1444	the composition	1430:1444	the composition of intestinal flora	1430:1464	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	0	35	from	effects	94:100	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	4	36	theme	α-Araf	713:718	arg1	residues					739:746	α-Araf, β-Galp and α-Rhap residues	713:746	α-Araf, β-Galp and α-Rhap residues at position C-3	713:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	9	37	theme	SCFAs	1488:1492	arg1	metabolism					1474:1483	the metabolism	1470:1483	the metabolism of SCFAs	1470:1492	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	9	37	theme	SCFAs	1488:1492	arg1	composition					1434:1444	the composition	1430:1444	the composition of intestinal flora	1430:1464	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	2	38	theme	active	397:402	arg1	ingredient					404:413	the specific active ingredient	384:413	the specific active ingredient of LBPs and its bioactivity	384:441	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	7	39	theme	specific	1125:1132	arg1	genera					1134:1139	their specific genera	1119:1139	their specific genera	1119:1139	Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera.					
34097961	5	40	theme	underlying	864:873	arg1	mechanisms					875:884	the underlying mechanisms	860:884	the underlying mechanisms	860:884	The objective of this study was to evaluate the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms.					
34097961	9	41	theme	intestinal	1449:1458	arg1	flora					1460:1464	intestinal flora	1449:1464	intestinal flora	1449:1464	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	6	42	dep	In	887:888	arg1	vivo					890:893	vivo	890:893	vivo	890:893	In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly.					
34097961	4	43	theme	repeated	646:653	arg1	unit					655:658	a repeated unit	644:658	a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3	644:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	2	44	theme	specific	388:395	arg1	ingredient					404:413	the specific active ingredient	384:413	the specific active ingredient of LBPs and its bioactivity	384:441	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	9	45	theme	homogeneous	1309:1319	arg1	polysaccharide					1321:1334	the homogeneous polysaccharide	1305:1334	the homogeneous polysaccharide purified from L. barbarum	1305:1360	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	9	45	theme	homogeneous	1309:1319	arg1	agent					1391:1395	a prebiotic agent	1379:1395	a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs	1379:1492	In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs.					
34097961	6	46	theme	HFD-induced	959:969	arg1	obesity					976:982	HFD-induced mice obesity	959:982	HFD-induced mice obesity	959:982	In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly.					
34097961	0	47	theme	Structural	51:60	arg1	characterizations					62:78	Structural characterizations	51:78	Structural characterizations	51:78	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	6	48	theme	efficacy	895:902	arg1	trial					904:908	In vivo efficacy trial	887:908	In vivo efficacy trial	887:908	In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly.					
34097961	0	49	from	characterizations	62:78	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	7	50	theme	Gut	999:1001	arg1	analysis					1014:1021	Gut microbiota analysis	999:1021	Gut microbiota analysis	999:1021	Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera.					
34097961	4	51	theme	1 → residues	674:685	arg1	unit					655:658	a repeated unit	644:658	a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3	644:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	2	52	theme	bioactivity	431:441	arg1	ingredient					404:413	the specific active ingredient	384:413	the specific active ingredient of LBPs and its bioactivity	384:441	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	7	53	theme	intestinal	1082:1091	arg1	flora					1093:1097	intestinal flora	1082:1097	intestinal flora	1082:1097	Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera.					
34097961	2	54	theme	relevant	314:321	arg1	researches					323:332	the relevant researches	310:332	the relevant researches	310:332	However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored.					
34097961	6	55	theme	In	887:888	arg1	trial					904:908	In vivo efficacy trial	887:908	In vivo efficacy trial	887:908	In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly.					
34097961	7	56	theme	microbiota	1003:1012	arg1	analysis					1014:1021	Gut microbiota analysis	999:1021	Gut microbiota analysis	999:1021	Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera.					
34097961	4	57	theme	position	751:758	arg1	C-3					760:762	position C-3	751:762	position C-3	751:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	1	58	from	effects	203:209	arg1	diabetes					214:221	diabetes	214:221	diabetes	214:221	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	59	theme	Lycium	133:138	arg1	LBPs					166:169	LBPs	166:169	LBPs	166:169	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	59	theme	Lycium	133:138	arg1	polysaccharides					149:163	Lycium barbarum polysaccharides	133:163	Lycium barbarum polysaccharides (LBPs)	133:170	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	0	60	from	impacts	106:112	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	0	61	dep	polysaccharide	14:27	arg1	impacts					106:112	impacts	106:112	impacts on gut microbiota	106:130	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	0	61	dep	polysaccharide	14:27	arg1	characterizations					62:78	Structural characterizations	51:78	Structural characterizations	51:78	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	0	61	dep	polysaccharide	14:27	arg1	effects					94:100	anti-obesity effects	81:100	anti-obesity effects	81:100	A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota.					
34097961	1	62	theme	NAFLD	224:228	arg1	effects					203:209	their beneficial effects	186:209	their beneficial effects on diabetes	186:221	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	62	theme	NAFLD	224:228	arg1	diseases					260:267	NAFLD and related chronic metabolic diseases	224:267	NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD)	224:298	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	4	63	theme	main	619:622	arg1	chain					624:628	a main chain	617:628	a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3	617:762	Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3.					
34097961	5	64	theme	LBP-W	839:843	arg1	effect					829:834	the anti-obesogenic effect	809:834	the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms	809:884	The objective of this study was to evaluate the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms.					
34097961	8	65	theme	acids	1204:1208	arg1	content					1175:1181	the content	1171:1181	the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora	1171:1254	Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora.					
34097961	1	66	theme	related	234:240	arg1	effects					203:209	their beneficial effects	186:209	their beneficial effects on diabetes	186:221	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
34097961	1	66	theme	related	234:240	arg1	diseases					260:267	NAFLD and related chronic metabolic diseases	224:267	NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD)	224:298	Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD).					
33418952	0	0	theme	Cell-Scaffold	79:91	arg1	Interface					93:101	Cell-Scaffold Interface	79:101	Cell-Scaffold Interface	79:101	Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface.					
33418952	2	1	theme	tissue-implant	475:488	arg1	interaction					490:500	tissue-implant interaction	475:500	tissue-implant interaction	475:500	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	1	theme	tissue-implant	475:488	arg1	products					451:458	products evaluation and tissue-implant interaction	451:500	products evaluation and tissue-implant interaction	451:500	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	6	2	theme	protein	1073:1079	arg1	adsorption					1081:1090	protein adsorption	1073:1090	protein adsorption	1073:1090	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	2	3	from	present	554:560	arg1	science					389:395	science	389:395	science	389:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	3	from	present	554:560	arg1	domain					371:376	this rapidly advancing domain	348:376	this rapidly advancing domain of medical science	348:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	7	4	theme	composite-cells	1413:1427	arg1	interaction					1429:1439	composite-cells interaction	1413:1439	composite-cells interaction	1413:1439	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	4	5	theme	cell-seeded	852:862	arg1	samples					878:884	the cell-seeded and untreated samples	848:884	the cell-seeded and untreated samples	848:884	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	4	6	from	information	754:764	arg1	differences					779:789	molecular differences	769:789	molecular differences	769:789	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	1	7	theme	three-compound	173:186	arg1	biomaterial					206:216	three-compound ceramic composite biomaterial	173:216	three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP)	173:342	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	2	8	theme	bone	516:519	arg1	process					531:537	this case bone formation process	506:537	this case bone formation process	506:537	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	6	9	from	Deposition	1012:1021	arg1	crystallinity					1048:1060	its crystallinity	1044:1060	its crystallinity along with protein adsorption	1044:1090	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	6	10	theme	cell	1150:1153	arg1	samples					1155:1161	both mesenchymal stem cell samples	1128:1161	both mesenchymal stem cell samples	1128:1161	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	4	11	theme	Raman	704:708	arg1	imaging					724:730	Raman spectroscopic imaging	704:730	Raman spectroscopic imaging	704:730	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	2	12	theme	case	511:514	arg1	process					531:537	this case bone formation process	506:537	this case bone formation process	506:537	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	13	dep	products	451:458	arg1	interaction					490:500	tissue-implant interaction	475:500	tissue-implant interaction	475:500	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	13	dep	products	451:458	arg1	products					451:458	products evaluation and tissue-implant interaction	451:500	products evaluation and tissue-implant interaction	451:500	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	13	dep	products	451:458	arg1	evaluation					460:469	evaluation	460:469	evaluation	460:469	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	5	14	theme	composite	959:967	arg1	biocompatibility					969:984	composite biocompatibility	959:984	composite biocompatibility	959:984	With the use of this, it was possible to confirm composite biocompatibility and bioactivity in vitro.					
33418952	7	15	from	engineering	1335:1345	arg1	terms					1350:1354	terms	1350:1354	terms of both the organic and inorganic components considering composite-cells interaction	1350:1439	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	2	16	located	present	554:560	arg1	science					389:395	science	389:395	science	389:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	16	located	present	554:560	arg2	urge					402:405	the urge	398:405	the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process,	398:538	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	16	located	present	554:560	arg2	present					554:560	present	554:560	present	554:560	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	16	located	present	554:560	arg1	domain					371:376	this rapidly advancing domain	348:376	this rapidly advancing domain of medical science	348:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	3	17	theme	composite	685:693	arg1	surface					695:701	composite surface	685:701	composite surface	685:701	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	7	18	theme	organic	1368:1374	arg1	components					1390:1399	both the organic and inorganic components	1359:1399	components	1390:1399	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	7	19	theme	imaging	1303:1309	arg1	usefulness					1269:1278	The usefulness	1265:1278	The usefulness of spectroscopic Raman imaging	1265:1309	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	1	20	theme	ceramic	188:194	arg1	biomaterial					206:216	three-compound ceramic composite biomaterial	173:216	three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP)	173:342	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	4	21	theme	advantageous	741:752	arg1	information					754:764	advantageous information	741:764	advantageous information on molecular differences	741:789	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	1	22	theme	composite	196:204	arg1	biomaterial					206:216	three-compound ceramic composite biomaterial	173:216	three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP)	173:342	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	2	23	theme	specific	431:438	arg1	method					440:445	quick, reliable and specific method	411:445	quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process,	411:538	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	24	from	products	451:458	arg1	process					531:537	this case bone formation process	506:537	this case bone formation process	506:537	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	6	25	theme	stem	1145:1148	arg1	samples					1155:1161	both mesenchymal stem cell samples	1128:1161	both mesenchymal stem cell samples	1128:1161	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	2	26	attach	present	554:560	arg1	science					389:395	science	389:395	science	389:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	26	attach	present	554:560	arg2	urge					402:405	the urge	398:405	the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process,	398:538	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	26	attach	present	554:560	arg2	present					554:560	present	554:560	present	554:560	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	26	attach	present	554:560	arg1	domain					371:376	this rapidly advancing domain	348:376	this rapidly advancing domain of medical science	348:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	3	27	link	adipose-derived	588:602	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	27	link	adipose-derived	588:602	arg1	ADSCs					616:620	ADSCs	616:620	ADSCs	616:620	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	27	link	adipose-derived	588:602	arg1	cells					609:613	adipose-derived stem cells	588:613	adipose-derived stem cells (ADSCs)	588:621	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	28	theme	marrow-derived	632:645	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	28	theme	marrow-derived	632:645	arg1	BMDSCs					659:664	BMDSCs	659:664	BMDSCs	659:664	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	28	theme	marrow-derived	632:645	arg1	cells					652:656	bone marrow-derived stem cells	627:656	bone marrow-derived stem cells (BMDSCs)	627:665	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	2	29	from	domain	371:376	arg1	present					554:560	present	554:560	present	554:560	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	29	from	domain	371:376	arg1	urge					402:405	the urge	398:405	the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process,	398:538	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	5	30	theme	this	926:929	arg1	use					919:921	the use	915:921	the use of this	915:929	With the use of this, it was possible to confirm composite biocompatibility and bioactivity in vitro.					
33418952	0	31	theme	Spectroscopic	21:33	arg1	Imaging					35:41	Raman Spectroscopic Imaging	15:41	Raman Spectroscopic Imaging	15:41	Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface.					
33418952	6	32	from	changes	1033:1039	arg1	crystallinity					1048:1060	its crystallinity	1044:1060	its crystallinity along with protein adsorption	1044:1090	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	1	33	theme	Raman	104:108	arg1	imaging					124:130	Raman spectroscopic imaging	104:130	Raman spectroscopic imaging	104:130	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	6	34	theme	biomineralised	1222:1235	arg1	ECM					1259:1261	ECM	1259:1261	ECM	1259:1261	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	6	34	theme	biomineralised	1222:1235	arg1	matrix					1251:1256	biomineralised extracellular matrix	1222:1256	biomineralised extracellular matrix (ECM)	1222:1262	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	2	35	theme	quick	411:415	arg1	method					440:445	quick, reliable and specific method	411:445	quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process,	411:538	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	0	36	theme	Raman	15:19	arg1	Imaging					35:41	Raman Spectroscopic Imaging	15:41	Raman Spectroscopic Imaging	15:41	Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface.					
33418952	7	37	dep	components	1390:1399	arg1	both					1359:1362	both	1359:1362	both	1359:1362	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	6	38	from	formation	1115:1123	arg1	samples					1155:1161	both mesenchymal stem cell samples	1128:1161	both mesenchymal stem cell samples	1128:1161	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	1	39	theme	spectroscopic	110:122	arg1	imaging					124:130	Raman spectroscopic imaging	104:130	Raman spectroscopic imaging	104:130	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	6	40	theme	mesenchymal	1133:1143	arg1	samples					1155:1161	both mesenchymal stem cell samples	1128:1161	both mesenchymal stem cell samples	1128:1161	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	7	41	theme	spectroscopic	1283:1295	arg1	imaging					1303:1309	spectroscopic Raman imaging	1283:1309	spectroscopic Raman imaging	1283:1309	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	1	42	theme	bone	306:309	arg1	TEP					339:341	TEP	339:341	TEP	339:341	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	1	42	theme	bone	306:309	arg1	product					330:336	a bone tissue engineering product	304:336	a bone tissue engineering product (TEP)	304:342	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	3	43	theme	stem	576:579	arg1	cells					581:585	stem cells	576:585	stem cells	576:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	7	44	theme	inorganic	1380:1388	arg1	components					1390:1399	both the organic and inorganic components	1359:1399	components	1390:1399	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	6	45	theme	HA	1026:1027	arg1	changes					1033:1039	changes	1033:1039	changes in its crystallinity along with protein adsorption	1033:1090	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	6	45	theme	HA	1026:1027	arg1	Deposition					1012:1021	Deposition	1012:1021	Deposition of HA	1012:1027	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	0	46	theme	Imaging	35:41	arg1	Application					0:10	Application	0:10	Application of Raman Spectroscopic Imaging	0:41	Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface.					
33418952	1	47	theme	tissue	311:316	arg1	TEP					339:341	TEP	339:341	TEP	339:341	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	1	47	theme	tissue	311:316	arg1	product					330:336	a bone tissue engineering product	304:336	a bone tissue engineering product (TEP)	304:342	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	2	48	theme	formation	521:529	arg1	process					531:537	this case bone formation process	506:537	this case bone formation process	506:537	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	49	theme	reliable	418:425	arg1	method					440:445	quick, reliable and specific method	411:445	quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process,	411:538	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	4	50	theme	spectroscopic	710:722	arg1	imaging					724:730	Raman spectroscopic imaging	704:730	Raman spectroscopic imaging	704:730	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	1	51	theme	engineering	318:328	arg1	TEP					339:341	TEP	339:341	TEP	339:341	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	1	51	theme	engineering	318:328	arg1	product					330:336	a bone tissue engineering product	304:336	a bone tissue engineering product (TEP)	304:342	Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP).					
33418952	4	52	theme	compounds	819:827	arg1	distribution					803:814	spatial distribution	795:814	spatial distribution of compounds	795:827	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	4	52	theme	compounds	819:827	arg1	information					754:764	advantageous information	741:764	advantageous information on molecular differences	741:789	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	6	53	theme	extracellular	1237:1249	arg1	ECM					1259:1261	ECM	1259:1261	ECM	1259:1261	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	6	53	theme	extracellular	1237:1249	arg1	matrix					1251:1256	biomineralised extracellular matrix	1222:1256	biomineralised extracellular matrix (ECM)	1222:1262	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	5	54	with	possible	939:946	arg1	use					919:921	the use	915:921	the use of this	915:929	With the use of this, it was possible to confirm composite biocompatibility and bioactivity in vitro.					
33418952	3	55	theme	stem	647:650	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	55	theme	stem	647:650	arg1	BMDSCs					659:664	BMDSCs	659:664	BMDSCs	659:664	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	55	theme	stem	647:650	arg1	cells					652:656	bone marrow-derived stem cells	627:656	bone marrow-derived stem cells (BMDSCs)	627:665	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	4	56	from	distribution	803:814	arg1	differences					779:789	molecular differences	769:789	molecular differences	769:789	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	6	57	theme	tissue	1108:1113	arg1	formation					1115:1123	new bone tissue formation	1099:1123	new bone tissue formation	1099:1123	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	4	58	theme	spatial	795:801	arg1	distribution					803:814	spatial distribution	795:814	spatial distribution of compounds	795:827	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	4	59	theme	molecular	769:777	arg1	differences					779:789	molecular differences	769:789	molecular differences	769:789	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	3	60	theme	stem	604:607	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	60	theme	stem	604:607	arg1	ADSCs					616:620	ADSCs	616:620	ADSCs	616:620	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	60	theme	stem	604:607	arg1	cells					609:613	adipose-derived stem cells	588:613	adipose-derived stem cells (ADSCs)	588:621	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	61	theme	cells	581:585	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	61	theme	cells	581:585	arg1	cells					609:613	adipose-derived stem cells	588:613	adipose-derived stem cells (ADSCs)	588:621	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	61	theme	cells	581:585	arg1	cells					652:656	bone marrow-derived stem cells	627:656	bone marrow-derived stem cells (BMDSCs)	627:665	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	6	62	theme	bone	1103:1106	arg1	formation					1115:1123	new bone tissue formation	1099:1123	new bone tissue formation	1099:1123	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	3	63	link	marrow-derived	632:645	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	63	link	marrow-derived	632:645	arg1	BMDSCs					659:664	BMDSCs	659:664	BMDSCs	659:664	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	63	link	marrow-derived	632:645	arg1	cells					652:656	bone marrow-derived stem cells	627:656	bone marrow-derived stem cells (BMDSCs)	627:665	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	7	64	theme	components	1390:1399	arg1	terms					1350:1354	terms	1350:1354	terms of both the organic and inorganic components considering composite-cells interaction	1350:1439	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	4	65	theme	microscopic	891:901	arg1	level					903:907	a microscopic level	889:907	a microscopic level	889:907	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	6	66	theme	new	1099:1101	arg1	formation					1115:1123	new bone tissue formation	1099:1123	new bone tissue formation	1099:1123	Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM).					
33418952	4	67	theme	untreated	868:876	arg1	samples					878:884	the cell-seeded and untreated samples	848:884	the cell-seeded and untreated samples	848:884	Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level.					
33418952	0	68	from	Interface	93:101	arg1	Changes					68:74	the Structural Changes	53:74	the Structural Changes at Cell-Scaffold Interface	53:101	Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface.					
33418952	7	69	theme	Raman	1297:1301	arg1	imaging					1303:1309	spectroscopic Raman imaging	1283:1309	spectroscopic Raman imaging	1283:1309	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	2	70	theme	science	389:395	arg1	science					389:395	science	389:395	science	389:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	70	theme	science	389:395	arg1	domain					371:376	this rapidly advancing domain	348:376	this rapidly advancing domain of medical science	348:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	7	71	theme	tissue	1328:1333	arg1	engineering					1335:1345	tissue engineering	1328:1345	tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction	1328:1439	The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite-cells interaction.					
33418952	0	72	theme	Structural	57:66	arg1	Changes					68:74	the Structural Changes	53:74	the Structural Changes at Cell-Scaffold Interface	53:101	Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface.					
33418952	3	73	theme	adipose-derived	588:602	arg1	types					567:571	Two types	563:571	Two types of stem cells	563:585	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	73	theme	adipose-derived	588:602	arg1	ADSCs					616:620	ADSCs	616:620	ADSCs	616:620	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	3	73	theme	adipose-derived	588:602	arg1	cells					609:613	adipose-derived stem cells	588:613	adipose-derived stem cells (ADSCs)	588:621	Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface.					
33418952	2	74	theme	advancing	361:369	arg1	science					389:395	science	389:395	science	389:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
33418952	2	74	theme	advancing	361:369	arg1	domain					371:376	this rapidly advancing domain	348:376	this rapidly advancing domain of medical science	348:395	In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue-implant interaction, in this case bone formation process, is constantly present.					
32575742	3	0	with	complexes	375:383	arg1	compounds					398:406	various compounds	390:406	various compounds	390:406	Cyclodextrins are well known for their ability to form inclusion complexes with various compounds to improve their solubility and stability.					
32575742	10	1	theme	complexes	1251:1259	arg1	diameter					1231:1238	the diameter	1227:1238	the diameter of γCD/PVA complexes	1227:1259	It was found that the diameter of γCD/PVA complexes increased with respect to the concentration of γCD.					
32575742	8	2	theme	PVA	1117:1119	arg1	ring					1087:1090	the heterocyclic ring	1070:1090	the heterocyclic ring of AA	1070:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	2	theme	PVA	1117:1119	arg1	PVA					1117:1119	PVA	1117:1119	PVA	1117:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	2	theme	PVA	1117:1119	arg1	AA					1095:1096	AA	1095:1096	AA	1095:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	2	theme	PVA	1117:1119	arg1	cavity					1137:1142	hydrophobic cavity	1125:1142	hydrophobic cavity	1125:1142	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	2	theme	PVA	1117:1119	arg1	group					1108:1112	hydroxyl group	1099:1112	hydroxyl group of PVA	1099:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	2	theme	PVA	1117:1119	arg1	γCD					1147:1149	γCD	1147:1149	γCD	1147:1149	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	1	3	theme	general	139:145	arg1	antioxidant					147:157	a general antioxidant	137:157	a general antioxidant used in aqueous pharmaceutical formulations	137:201	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	1	3	theme	general	139:145	arg1	acid					124:127	Ascorbic acid	115:127	Ascorbic acid (AA)	115:132	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	8	4	theme	heterocyclic	1074:1085	arg1	ring					1087:1090	the heterocyclic ring	1070:1090	the heterocyclic ring of AA	1070:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	6	5	theme	protective	802:811	arg1	ability					813:819	the protective ability	798:819	the protective ability of γCD on AA	798:832	This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA.					
32575742	8	6	theme	hydrophobic	1125:1135	arg1	cavity					1137:1142	hydrophobic cavity	1125:1142	hydrophobic cavity	1125:1142	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	15	7	theme	antioxidant	1785:1795	arg1	capacity					1797:1804	antioxidant capacity	1785:1804	antioxidant capacity	1785:1804	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	12	8	attach	presence	1418:1425	arg2	AA					1430:1431	AA	1430:1431	AA	1430:1431	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	12	8	attach	presence	1418:1425	arg1	complexes					1444:1452	ternary complexes	1436:1452	ternary complexes	1436:1452	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	6	9	with	complexes	867:875	arg1	PVA					882:884	PVA	882:884	PVA	882:884	This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA.					
32575742	15	10	theme	concentration	1768:1780	arg1	influence					1743:1751	The influence	1739:1751	The influence of γCD and PVA concentration on antioxidant capacity	1739:1804	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	1	11	theme	aqueous	167:173	arg1	formulations					190:201	aqueous pharmaceutical formulations	167:201	aqueous pharmaceutical formulations	167:201	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	12	12	theme	γCD/PVA	1498:1504	arg1	complexes					1513:1521	γCD/PVA binary complexes	1498:1521	γCD/PVA binary complexes	1498:1521	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	6	13	theme	ternary	859:865	arg1	complexes					867:875	ternary complexes	859:875	ternary complexes with PVA	859:884	This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA.					
32575742	2	14	from	unstable	241:248	arg1	solutions					224:232	aqueous solutions	216:232	aqueous solutions	216:232	However, in aqueous solutions, AA is unstable and easily oxidized when exposed to air, light and/or heat.					
32575742	14	15	theme	contrasting	1719:1729	arg1	effect					1731:1736	a contrasting effect	1717:1736	a contrasting effect	1717:1736	It was found that the presence of γCD preserved the antioxidant activity of AA, whereas PVA showed a contrasting effect.					
32575742	12	16	theme	AA	1430:1431	arg1	presence					1418:1425	The presence	1414:1425	The presence of AA in ternary complexes	1414:1452	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	7	17	dep	Binary	887:892	arg1	AA/PVA					909:914	AA/PVA	909:914	AA/PVA	909:914	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	7	17	dep	Binary	887:892	arg1	AA/γCD					901:906	AA/γCD	901:906	AA/γCD	901:906	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	7	17	dep	Binary	887:892	arg1	γCD/PVA					920:926	γCD/PVA	920:926	γCD/PVA	920:926	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	5	18	theme	various	680:686	arg1	fields					703:708	various pharmaceutical fields	680:708	various pharmaceutical fields	680:708	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	2	19	theme	aqueous	216:222	arg1	solutions					224:232	aqueous solutions	216:232	aqueous solutions	216:232	However, in aqueous solutions, AA is unstable and easily oxidized when exposed to air, light and/or heat.					
32575742	12	20	theme	ternary	1436:1442	arg1	complexes					1444:1452	ternary complexes	1436:1452	ternary complexes	1436:1452	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	13	21	theme	ternary	1582:1588	arg1	complexes					1590:1598	binary and ternary complexes	1571:1598	binary and ternary complexes	1571:1598	Furthermore, the antioxidant capacity of AA in binary and ternary complexes was investigated.					
32575742	11	22	theme	complex	1405:1411	arg1	diameters					1380:1388	hydrodynamic diameters	1367:1388	hydrodynamic diameters of the ternary complex	1367:1411	Higher γCD concentrations also resulted in increasing hydrodynamic diameters of the ternary complex.					
32575742	10	23	theme	γCD	1308:1310	arg1	concentration					1291:1303	the concentration	1287:1303	the concentration of γCD	1287:1310	It was found that the diameter of γCD/PVA complexes increased with respect to the concentration of γCD.					
32575742	17	24	theme	protective	2042:2051	arg1	complex					2061:2067	A promising protective ternary complex	2030:2067	A promising protective ternary complex	2030:2067	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	9	25	theme	complexes	1181:1189	arg1	diameter					1169:1176	The hydrodynamic diameter	1152:1176	The hydrodynamic diameter of complexes	1152:1189	The hydrodynamic diameter of complexes was then studied.					
32575742	17	26	theme	high	2127:2130	arg1	concentration					2132:2144	a high concentration	2125:2144	a high concentration of γCD	2125:2151	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	13	27	theme	AA	1565:1566	arg1	capacity					1553:1560	the antioxidant capacity	1537:1560	the antioxidant capacity of AA in binary and ternary complexes	1537:1598	Furthermore, the antioxidant capacity of AA in binary and ternary complexes was investigated.					
32575742	11	28	theme	Higher	1313:1318	arg1	concentrations					1324:1337	Higher γCD concentrations	1313:1337	Higher γCD concentrations	1313:1337	Higher γCD concentrations also resulted in increasing hydrodynamic diameters of the ternary complex.					
32575742	3	29	theme	various	390:396	arg1	compounds					398:406	various compounds	390:406	various compounds	390:406	Cyclodextrins are well known for their ability to form inclusion complexes with various compounds to improve their solubility and stability.					
32575742	0	30	theme	vinyl	99:103	arg1	Poly					94:97	Poly	94:97	Poly(vinyl Alcohol)	94:112	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	0	30	theme	vinyl	99:103	arg1	Alcohol					105:111	vinyl Alcohol	99:111	vinyl Alcohol	99:111	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	12	31	from	presence	1418:1425	arg1	complexes					1444:1452	ternary complexes	1436:1452	ternary complexes	1436:1452	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	13	32	theme	binary	1571:1576	arg1	complexes					1590:1598	binary and ternary complexes	1571:1598	binary and ternary complexes	1571:1598	Furthermore, the antioxidant capacity of AA in binary and ternary complexes was investigated.					
32575742	5	33	theme	drug	735:738	arg1	stabilization					740:752	drug stabilization	735:752	drug stabilization	735:752	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	2	34	from	solutions	224:232	arg1	unstable					241:248	unstable	241:248	unstable	241:248	However, in aqueous solutions, AA is unstable and easily oxidized when exposed to air, light and/or heat.					
32575742	16	35	theme	γCD	1896:1898	arg1	concentration					1879:1891	the concentration	1875:1891	the concentration of γCD	1875:1898	Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored.					
32575742	7	36	dep	AA/γCD	901:906	arg1	i.e.					895:898	i.e.	895:898	i.e.	895:898	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	0	37	theme	Ternary	35:41	arg1	Complexes					43:51	Ternary Complexes	35:51	Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol)	35:112	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	14	38	theme	antioxidant	1670:1680	arg1	activity					1682:1689	the antioxidant activity	1666:1689	the antioxidant activity of AA	1666:1695	It was found that the presence of γCD preserved the antioxidant activity of AA, whereas PVA showed a contrasting effect.					
32575742	16	39	theme	inhibition	1927:1936	arg1	efficiency					1938:1947	the inhibition efficiency	1923:1947	the inhibition efficiency of the ternary complex	1923:1970	Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored.					
32575742	0	40	theme	Acid	65:68	arg1	Complexes					43:51	Ternary Complexes	35:51	Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol)	35:112	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	4	41	theme	antioxidant	512:522	arg1	capacity					524:531	the antioxidant capacity	508:531	the antioxidant capacity of AA	508:537	Previous studies demonstrate that cyclodextrins preserve the antioxidant capacity of AA but data for γ-cyclodextrin (γCD) have not been reported.					
32575742	16	42	theme	ternary	1956:1962	arg1	complex					1964:1970	the ternary complex	1952:1970	the ternary complex	1952:1970	Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored.					
32575742	7	43	dep	AA/γCD/PVA	948:957	arg1	i.e.					942:945	i.e.	942:945	i.e.	942:945	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	5	44	theme	vinyl	602:606	arg1	Poly					597:600	Poly	597:600	Poly(vinyl alcohol) (PVA)	597:621	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	5	44	theme	vinyl	602:606	arg1	alcohol					608:614	vinyl alcohol	602:614	vinyl alcohol	602:614	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	13	45	from	capacity	1553:1560	arg1	complexes					1590:1598	binary and ternary complexes	1571:1598	binary and ternary complexes	1571:1598	Furthermore, the antioxidant capacity of AA in binary and ternary complexes was investigated.					
32575742	15	46	theme	composite	1839:1847	arg1	CCD					1857:1859	CCD	1857:1859	CCD	1857:1859	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	15	46	theme	composite	1839:1847	arg1	design					1849:1854	central composite design	1831:1854	central composite design (CCD)	1831:1860	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	6	47	from	ability	813:819	arg1	AA					831:832	AA	831:832	AA	831:832	This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA.					
32575742	6	48	theme	γCD	824:826	arg1	ability					813:819	the protective ability	798:819	the protective ability of γCD on AA	798:832	This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA.					
32575742	7	49	dep	ternary	933:939	arg1	AA/γCD/PVA					948:957	AA/γCD/PVA	948:957	AA/γCD/PVA	948:957	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	10	50	theme	γCD/PVA	1243:1249	arg1	complexes					1251:1259	γCD/PVA complexes	1243:1259	γCD/PVA complexes	1243:1259	It was found that the diameter of γCD/PVA complexes increased with respect to the concentration of γCD.					
32575742	16	51	theme	PVA	2004:2006	arg1	influence					1991:1999	the insignificant influence	1973:1999	the insignificant influence of PVA	1973:2006	Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored.					
32575742	15	52	theme	PVA	1764:1766	arg1	concentration					1768:1780	PVA concentration	1764:1780	PVA concentration	1764:1780	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	5	53	theme	hydrophilic	628:638	arg1	Poly					597:600	Poly	597:600	Poly(vinyl alcohol) (PVA)	597:621	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	5	53	theme	hydrophilic	628:638	arg1	polymer					640:646	a hydrophilic polymer	626:646	a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields	626:708	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	12	54	theme	binary	1506:1511	arg1	complexes					1513:1521	γCD/PVA binary complexes	1498:1521	γCD/PVA binary complexes	1498:1521	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	17	55	theme	γCD	2149:2151	arg1	concentration					2100:2112	an optimized concentration	2087:2112	an optimized concentration of PVA	2087:2119	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	17	55	theme	γCD	2149:2151	arg1	concentration					2132:2144	a high concentration	2125:2144	a high concentration of γCD	2125:2151	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	15	56	theme	γCD	1756:1758	arg1	influence					1743:1751	The influence	1739:1751	The influence of γCD and PVA concentration on antioxidant capacity	1739:1804	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	1	57	theme	pharmaceutical	175:188	arg1	formulations					190:201	aqueous pharmaceutical formulations	167:201	aqueous pharmaceutical formulations	167:201	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	8	58	theme	AA	1095:1096	arg1	ring					1087:1090	the heterocyclic ring	1070:1090	the heterocyclic ring of AA	1070:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	58	theme	AA	1095:1096	arg1	PVA					1117:1119	PVA	1117:1119	PVA	1117:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	58	theme	AA	1095:1096	arg1	AA					1095:1096	AA	1095:1096	AA	1095:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	58	theme	AA	1095:1096	arg1	cavity					1137:1142	hydrophobic cavity	1125:1142	hydrophobic cavity	1125:1142	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	58	theme	AA	1095:1096	arg1	group					1108:1112	hydroxyl group	1099:1112	hydroxyl group of PVA	1099:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	58	theme	AA	1095:1096	arg1	γCD					1147:1149	γCD	1147:1149	γCD	1147:1149	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	6	59	theme	complexes	867:875	arg1	formation					846:854	the formation	842:854	the formation of ternary complexes with PVA	842:884	This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA.					
32575742	8	60	theme	γCD	1147:1149	arg1	ring					1087:1090	the heterocyclic ring	1070:1090	the heterocyclic ring of AA	1070:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	60	theme	γCD	1147:1149	arg1	PVA					1117:1119	PVA	1117:1119	PVA	1117:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	60	theme	γCD	1147:1149	arg1	AA					1095:1096	AA	1095:1096	AA	1095:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	60	theme	γCD	1147:1149	arg1	cavity					1137:1142	hydrophobic cavity	1125:1142	hydrophobic cavity	1125:1142	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	60	theme	γCD	1147:1149	arg1	group					1108:1112	hydroxyl group	1099:1112	hydroxyl group of PVA	1099:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	60	theme	γCD	1147:1149	arg1	γCD					1147:1149	γCD	1147:1149	γCD	1147:1149	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	7	61	theme	ternary	933:939	arg1	complexes					960:968	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes	887:968	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes	887:968	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	8	62	theme	hydroxyl	1099:1106	arg1	γCD					1147:1149	γCD	1147:1149	γCD	1147:1149	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	62	theme	hydroxyl	1099:1106	arg1	PVA					1117:1119	PVA	1117:1119	PVA	1117:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	62	theme	hydroxyl	1099:1106	arg1	AA					1095:1096	AA	1095:1096	AA	1095:1096	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	8	62	theme	hydroxyl	1099:1106	arg1	group					1108:1112	hydroxyl group	1099:1112	hydroxyl group of PVA	1099:1119	It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD.					
32575742	12	63	theme	aggregation	1474:1484	arg1	tendency					1486:1493	the aggregation tendency	1470:1493	the aggregation tendency of γCD/PVA binary complexes	1470:1521	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	11	64	theme	hydrodynamic	1367:1378	arg1	diameters					1380:1388	hydrodynamic diameters	1367:1388	hydrodynamic diameters of the ternary complex	1367:1411	Higher γCD concentrations also resulted in increasing hydrodynamic diameters of the ternary complex.					
32575742	1	65	theme	Ascorbic	115:122	arg1	antioxidant					147:157	a general antioxidant	137:157	a general antioxidant used in aqueous pharmaceutical formulations	137:201	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	1	65	theme	Ascorbic	115:122	arg1	AA					130:131	AA	130:131	AA	130:131	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	1	65	theme	Ascorbic	115:122	arg1	acid					124:127	Ascorbic acid	115:127	Ascorbic acid (AA)	115:132	Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations.					
32575742	11	66	theme	ternary	1397:1403	arg1	complex					1405:1411	the ternary complex	1393:1411	the ternary complex	1393:1411	Higher γCD concentrations also resulted in increasing hydrodynamic diameters of the ternary complex.					
32575742	5	67	theme	pharmaceutical	688:701	arg1	fields					703:708	various pharmaceutical fields	680:708	various pharmaceutical fields	680:708	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	9	68	theme	hydrodynamic	1156:1167	arg1	diameter					1169:1176	The hydrodynamic diameter	1152:1176	The hydrodynamic diameter of complexes	1152:1189	The hydrodynamic diameter of complexes was then studied.					
32575742	17	69	theme	PVA	2117:2119	arg1	concentration					2100:2112	an optimized concentration	2087:2112	an optimized concentration of PVA	2087:2119	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	17	69	theme	PVA	2117:2119	arg1	concentration					2132:2144	a high concentration	2125:2144	a high concentration of γCD	2125:2151	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	15	70	theme	central	1831:1837	arg1	CCD					1857:1859	CCD	1857:1859	CCD	1857:1859	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	15	70	theme	central	1831:1837	arg1	design					1849:1854	central composite design	1831:1854	central composite design (CCD)	1831:1860	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	7	71	theme	Binary	887:892	arg1	complexes					960:968	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes	887:968	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes	887:968	Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed.					
32575742	16	72	theme	complex	1964:1970	arg1	efficiency					1938:1947	the inhibition efficiency	1923:1947	the inhibition efficiency of the ternary complex	1923:1970	Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored.					
32575742	17	73	theme	ternary	2053:2059	arg1	complex					2061:2067	A promising protective ternary complex	2030:2067	A promising protective ternary complex	2030:2067	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	5	74	theme	drug	665:668	arg1	matrix					670:675	a drug matrix	663:675	a drug matrix	663:675	Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited.					
32575742	4	75	theme	Previous	451:458	arg1	studies					460:466	Previous studies	451:466	Previous studies	451:466	Previous studies demonstrate that cyclodextrins preserve the antioxidant capacity of AA but data for γ-cyclodextrin (γCD) have not been reported.					
32575742	4	76	theme	AA	536:537	arg1	capacity					524:531	the antioxidant capacity	508:531	the antioxidant capacity of AA	508:537	Previous studies demonstrate that cyclodextrins preserve the antioxidant capacity of AA but data for γ-cyclodextrin (γCD) have not been reported.					
32575742	16	77	theme	insignificant	1977:1989	arg1	influence					1991:1999	the insignificant influence	1973:1999	the insignificant influence of PVA	1973:2006	Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored.					
32575742	14	78	theme	AA	1694:1695	arg1	activity					1682:1689	the antioxidant activity	1666:1689	the antioxidant activity of AA	1666:1695	It was found that the presence of γCD preserved the antioxidant activity of AA, whereas PVA showed a contrasting effect.					
32575742	12	79	theme	complexes	1513:1521	arg1	tendency					1486:1493	the aggregation tendency	1470:1493	the aggregation tendency of γCD/PVA binary complexes	1470:1521	The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes.					
32575742	13	80	theme	antioxidant	1541:1551	arg1	capacity					1553:1560	the antioxidant capacity	1537:1560	the antioxidant capacity of AA in binary and ternary complexes	1537:1598	Furthermore, the antioxidant capacity of AA in binary and ternary complexes was investigated.					
32575742	17	81	theme	optimized	2090:2098	arg1	concentration					2100:2112	an optimized concentration	2087:2112	an optimized concentration of PVA	2087:2119	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	11	82	theme	γCD	1320:1322	arg1	concentrations					1324:1337	Higher γCD concentrations	1313:1337	Higher γCD concentrations	1313:1337	Higher γCD concentrations also resulted in increasing hydrodynamic diameters of the ternary complex.					
32575742	0	83	theme	Complexes	43:51	arg1	Evaluation					21:30	Evaluation	21:30	Evaluation	21:30	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	0	83	theme	Complexes	43:51	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	3	84	theme	inclusion	365:373	arg1	complexes					375:383	inclusion complexes	365:383	inclusion complexes with various compounds	365:406	Cyclodextrins are well known for their ability to form inclusion complexes with various compounds to improve their solubility and stability.					
32575742	0	85	theme	Ascorbic	56:63	arg1	Acid					65:68	Ascorbic Acid	56:68	Ascorbic Acid	56:68	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	14	86	theme	γCD	1652:1654	arg1	presence					1640:1647	the presence	1636:1647	the presence of γCD	1636:1654	It was found that the presence of γCD preserved the antioxidant activity of AA, whereas PVA showed a contrasting effect.					
32575742	15	87	from	influence	1743:1751	arg1	capacity					1797:1804	antioxidant capacity	1785:1804	antioxidant capacity	1785:1804	The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD).					
32575742	17	88	theme	promising	2032:2040	arg1	complex					2061:2067	A promising protective ternary complex	2030:2067	A promising protective ternary complex	2030:2067	A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.					
32575742	0	89	with	Complexes	43:51	arg1	γ-Cyclodextrin					75:88	γ-Cyclodextrin	75:88	γ-Cyclodextrin	75:88	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	0	89	with	Complexes	43:51	arg1	Poly					94:97	Poly	94:97	Poly(vinyl Alcohol)	94:112	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32575742	0	89	with	Complexes	43:51	arg1	Alcohol					105:111	vinyl Alcohol	99:111	vinyl Alcohol	99:111	Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol).					
32506705	4	0	theme	HA	780:781	arg1	doses					771:775	4 doses	769:775	4 doses of HA (6, 60, 600 and 6,000 µg/ml)	769:810	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	2	1	from	present	383:389	arg1	fluids					400:405	these fluids	394:405	these fluids	394:405	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	1	2	theme	essential	283:291	arg1	modifications					268:280	sperm cell modifications	257:280	sperm cell modifications	257:280	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	8	3	theme	HA	1498:1499	arg1	supplementation					1501:1515	HA supplementation	1498:1515	HA supplementation	1498:1515	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	8	4	theme	spermatozoa	1689:1699	arg1	number					1649:1654	the number	1645:1654	the number of motile, rapid and progressive spermatozoa	1645:1699	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	5	5	from	bulls	904:908	arg1	semen					891:895	thawed semen	884:895	thawed semen from 6 bulls	884:908	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	1	6	theme	uterine	180:186	arg1	fluids					200:205	the uterine and oviduct fluids	176:205	fluids	200:205	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	9	7	theme	rheological	1747:1757	arg1	properties					1759:1768	the rheological properties	1743:1768	the rheological properties of the fertilization medium	1743:1796	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	3	8	theme	sperm	662:666	arg1	motility					668:675	sperm motility	662:675	sperm motility	662:675	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	1	9	theme	oviduct	192:198	arg1	fluids					200:205	the uterine and oviduct fluids	176:205	fluids	200:205	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	9	10	theme	fertilization	1777:1789	arg1	medium					1791:1796	the fertilization medium	1773:1796	the fertilization medium	1773:1796	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	1	11	theme	female	116:121	arg1	tract					136:140	The female reproductive tract	112:140	The female reproductive tract	112:140	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	1	11	theme	female	116:121	arg1	responsible					211:221	responsible	211:221	responsible	211:221	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	1	12	from	responsible	211:221	arg1	part					236:239	part	236:239	part	236:239	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	7	13	theme	plasma	1460:1465	arg1	integrities					1476:1486	acrosomal and plasma membrane integrities	1446:1486	integrities	1476:1486	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	4	14	theme	strength	852:859	arg1	characteristics					861:875	adhesion strength characteristics	843:875	adhesion strength characteristics	843:875	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	0	15	theme	impact	64:69	arg1	motility					82:89	impact bull sperm motility	64:89	impact bull sperm motility	64:89	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	7	16	theme	acrosomal	1446:1454	arg1	integrities					1476:1486	acrosomal and plasma membrane integrities	1446:1486	integrities	1476:1486	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	1	17	theme	sperm	257:261	arg1	modifications					268:280	sperm cell modifications	257:280	sperm cell modifications	257:280	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	0	18	theme	sperm	76:80	arg1	motility					82:89	impact bull sperm motility	64:89	impact bull sperm motility	64:89	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	6	19	theme	rheological	1151:1161	arg1	evaluation					1163:1172	The rheological evaluation	1147:1172	The rheological evaluation	1147:1172	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	0	20	from	Changes	0:6	arg1	viscosity					32:40	fertilization medium viscosity	11:40	fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status	11:109	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	1	21	theme	ability	330:336	arg1	acquisition					301:311	the acquisition	297:311	the acquisition of fertilization ability	297:336	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	7	22	theme	mitochondrial	1417:1429	arg1	potential					1431:1439	mitochondrial potential	1417:1439	mitochondrial potential	1417:1439	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	9	23	theme	related	1845:1851	arg1	properties					1834:1843	the properties	1830:1843	the properties related to sperm motility and capacitation	1830:1886	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	8	24	theme	motile	1659:1664	arg1	spermatozoa					1689:1699	motile, rapid and progressive spermatozoa	1659:1699	motile, rapid and progressive spermatozoa	1659:1699	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	1	25	theme	cell	263:266	arg1	modifications					268:280	sperm cell modifications	257:280	sperm cell modifications	257:280	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	2	26	theme	sperm	454:458	arg1	functionality					460:472	sperm functionality	454:472	sperm functionality	454:472	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	8	27	from	increase	1544:1551	arg1	number					1560:1565	the number	1556:1565	the number of reacted sperm	1556:1582	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	8	27	from	increase	1544:1551	arg1	changes					1596:1602	changes	1596:1602	changes in motility parameters	1596:1625	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	8	28	theme	rapid	1667:1671	arg1	spermatozoa					1689:1699	motile, rapid and progressive spermatozoa	1659:1699	motile, rapid and progressive spermatozoa	1659:1699	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	0	29	theme	fertilization	11:23	arg1	viscosity					32:40	fertilization medium viscosity	11:40	fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status	11:109	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	5	30	theme	mitochondrial	1064:1076	arg1	potential					1087:1095	mitochondrial membrane potential	1064:1095	mitochondrial membrane potential	1064:1095	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	2	31	attach	present	383:389	arg2	acid					350:353	Hyaluronic acid	339:353	Hyaluronic acid (HA)	339:358	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	2	31	attach	present	383:389	arg2	glycosaminoglycan					365:381	a glycosaminoglycan	363:381	a glycosaminoglycan present in these fluids	363:405	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	2	31	attach	present	383:389	arg1	fluids					400:405	these fluids	394:405	these fluids	394:405	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	4	32	theme	fertilization	726:738	arg1	medium					740:745	the in vitro fertilization medium	713:745	the in vitro fertilization medium	713:745	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	6	33	theme	medium	1292:1297	arg1	viscosity					1235:1243	the viscosity	1231:1243	the viscosity	1231:1243	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	6	33	theme	medium	1292:1297	arg1	strength					1262:1269	the adhesion strength	1249:1269	the adhesion strength of the fertilization medium	1249:1297	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	7	34	theme	HA	1393:1394	arg1	influence					1380:1388	No influence	1377:1388	No influence of HA	1377:1394	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	3	35	theme	medium	595:600	arg1	characteristics					558:572	the rheological characteristics	542:572	the rheological characteristics of the fertilization medium	542:600	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	0	36	theme	hyaluronic	48:57	arg1	acid					59:62	hyaluronic acid impact bull sperm motility and acrosome status	48:109	hyaluronic acid impact bull sperm motility and acrosome status	48:109	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	5	37	theme	acrosomal	1032:1040	arg1	integrities					1051:1061	acrosomal membrane integrities	1032:1061	acrosomal membrane integrities	1032:1061	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	9	38	theme	functional	1916:1925	arg1	aspects					1927:1933	other functional aspects	1910:1933	other functional aspects of the cell	1910:1945	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	5	39	theme	acrosomal	1112:1120	arg1	reaction					1122:1129	acrosomal reaction	1112:1129	acrosomal reaction	1112:1129	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	6	40	from	group	1330:1334	arg1	effect					1349:1354	the effect	1345:1354	the effect	1345:1354	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	6	40	from	group	1330:1334	arg1	pronounced					1365:1374	pronounced	1365:1374	pronounced	1365:1374	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	4	41	theme	in	717:718	arg1	fertilization					726:738	the in vitro fertilization	713:738	the in vitro fertilization medium	713:745	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	9	42	theme	cell	1942:1945	arg1	aspects					1927:1933	other functional aspects	1910:1933	other functional aspects of the cell	1910:1945	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	8	43	theme	progressive	1677:1687	arg1	spermatozoa					1689:1699	motile, rapid and progressive spermatozoa	1659:1699	motile, rapid and progressive spermatozoa	1659:1699	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	9	44	dep	sperm	1856:1860	arg1	motility					1862:1869	motility	1862:1869	motility	1862:1869	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	4	45	dep	in	717:718	arg1	vitro					720:724	vitro	720:724	vitro	720:724	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	9	46	theme	HA	1733:1734	arg1	addition					1721:1728	the addition	1717:1728	the addition of HA	1717:1734	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	8	47	from	changes	1596:1602	arg1	parameters					1616:1625	motility parameters	1607:1625	motility parameters	1607:1625	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	3	48	theme	HA	646:647	arg1	influence					649:657	the HA influence	642:657	the HA influence	642:657	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	3	49	dep	determine	532:540	arg1	a					529:529	a	529:529	a	529:529	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	9	50	theme	other	1910:1914	arg1	aspects					1927:1933	other functional aspects	1910:1933	other functional aspects of the cell	1910:1945	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	3	51	theme	functional	681:690	arg1	status					692:697	functional status	681:697	functional status	681:697	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	1	52	from	part	236:239	arg1	tract					136:140	The female reproductive tract	112:140	The female reproductive tract	112:140	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	1	52	from	part	236:239	arg1	responsible					211:221	responsible	211:221	responsible	211:221	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	8	53	theme	reacted	1570:1576	arg1	sperm					1578:1582	reacted sperm	1570:1582	reacted sperm	1570:1582	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	7	54	located	observed	1405:1412	arg1	potential					1431:1439	mitochondrial potential	1417:1439	mitochondrial potential	1417:1439	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	7	54	located	observed	1405:1412	arg1	integrities					1476:1486	acrosomal and plasma membrane integrities	1446:1486	integrities	1476:1486	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	7	54	located	observed	1405:1412	arg2	influence					1380:1388	No influence	1377:1388	No influence of HA	1377:1394	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	8	55	theme	sperm	1578:1582	arg1	number					1560:1565	the number	1556:1565	the number of reacted sperm	1556:1582	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	8	55	theme	sperm	1578:1582	arg1	changes					1596:1602	changes	1596:1602	changes in motility parameters	1596:1625	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	1	56	theme	fluids	200:205	arg1	composition					161:171	particular the composition	146:171	particular the composition of the uterine and oviduct fluids	146:205	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	6	57	theme	HA	1202:1203	arg1	able					1209:1212	able	1209:1212	able	1209:1212	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	6	57	theme	HA	1202:1203	arg1	addition					1190:1197	the addition	1186:1197	the addition of HA	1186:1203	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	7	58	theme	membrane	1467:1474	arg1	integrities					1476:1486	acrosomal and plasma membrane integrities	1446:1486	integrities	1476:1486	No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities.					
32506705	9	59	theme	medium	1791:1796	arg1	properties					1759:1768	the rheological properties	1743:1768	the rheological properties of the fertilization medium	1743:1796	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	1	60	theme	reproductive	123:134	arg1	tract					136:140	The female reproductive tract	112:140	The female reproductive tract	112:140	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	1	60	theme	reproductive	123:134	arg1	responsible					211:221	responsible	211:221	responsible	211:221	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	8	61	from	increase	1633:1640	arg1	number					1649:1654	the number	1645:1654	the number of motile, rapid and progressive spermatozoa	1645:1699	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	0	62	theme	bull	71:74	arg1	motility					82:89	impact bull sperm motility	64:89	impact bull sperm motility	64:89	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	3	63	theme	rheological	546:556	arg1	characteristics					558:572	the rheological characteristics	542:572	the rheological characteristics of the fertilization medium	542:600	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	8	64	theme	motility	1607:1614	arg1	parameters					1616:1625	motility parameters	1607:1625	motility parameters	1607:1625	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	4	65	theme	adhesion	843:850	arg1	characteristics					861:875	adhesion strength characteristics	843:875	adhesion strength characteristics	843:875	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	2	66	from	fluids	400:405	arg1	present					383:389	present	383:389	present	383:389	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	6	67	theme	adhesion	1253:1260	arg1	strength					1262:1269	the adhesion strength	1249:1269	the adhesion strength of the fertilization medium	1249:1297	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	5	68	theme	functional	998:1007	arg1	parameters					1009:1018	morphological and functional parameters	980:1018	morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility)	980:1144	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	1	69	theme	particular	146:155	arg1	composition					161:171	particular the composition	146:171	particular the composition of the uterine and oviduct fluids	146:205	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	5	70	theme	different	958:966	arg1	moments					968:974	4 different moments	956:974	4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility)	956:1144	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	2	71	theme	fertilization	428:440	arg1	process					442:448	the fertilization process	424:448	the fertilization process	424:448	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	0	72	theme	acrosome	95:102	arg1	status					104:109	acrosome status	95:109	acrosome status	95:109	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	1	73	theme	fertilization	316:328	arg1	ability					330:336	fertilization ability	316:336	fertilization ability	316:336	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	2	74	from	role	416:419	arg1	process					442:448	the fertilization process	424:448	the fertilization process	424:448	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	2	74	from	role	416:419	arg1	functionality					460:472	sperm functionality	454:472	sperm functionality	454:472	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	9	75	theme	properties	1834:1843	arg1	improvement					1815:1825	the improvement	1811:1825	the improvement of the properties related to sperm motility and capacitation	1811:1886	In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.					
32506705	5	76	dep	plasma	1021:1026	arg1	capacitation					1098:1109	capacitation	1098:1109	capacitation	1098:1109	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	5	76	dep	plasma	1021:1026	arg1	reaction					1122:1129	acrosomal reaction	1112:1129	acrosomal reaction	1112:1129	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	5	76	dep	plasma	1021:1026	arg1	potential					1087:1095	mitochondrial membrane potential	1064:1095	mitochondrial membrane potential	1064:1095	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	5	76	dep	plasma	1021:1026	arg1	motility					1136:1143	motility	1136:1143	motility	1136:1143	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	4	77	dep	doses	771:775	arg1	6,000 µg/ml					799:809	6,000 µg/ml	799:809	6,000 µg/ml	799:809	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	4	77	dep	doses	771:775	arg1	600					791:793	600	791:793	600	791:793	To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 µg/ml) and analysed for viscosity and adhesion strength characteristics.					
32506705	5	78	theme	morphological	980:992	arg1	parameters					1009:1018	morphological and functional parameters	980:1018	morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility)	980:1144	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	1	79	from	tract	136:140	arg1	composition					161:171	particular the composition	146:171	particular the composition of the uterine and oviduct fluids	146:205	The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability.					
32506705	5	80	dep	parameters	1009:1018	arg1	plasma					1021:1026	plasma	1021:1026	plasma	1021:1026	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	5	80	dep	parameters	1009:1018	arg1	integrities					1051:1061	acrosomal membrane integrities	1032:1061	acrosomal membrane integrities	1032:1061	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	0	81	theme	medium	25:30	arg1	viscosity					32:40	fertilization medium viscosity	11:40	fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status	11:109	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	2	82	theme	present	383:389	arg1	glycosaminoglycan					365:381	a glycosaminoglycan	363:381	a glycosaminoglycan present in these fluids	363:405	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	2	82	theme	present	383:389	arg1	acid					350:353	Hyaluronic acid	339:353	Hyaluronic acid (HA)	339:358	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	5	83	theme	membrane	1078:1085	arg1	potential					1087:1095	mitochondrial membrane potential	1064:1095	mitochondrial membrane potential	1064:1095	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	0	84	dep	acid	59:62	arg1	motility					82:89	impact bull sperm motility	64:89	impact bull sperm motility	64:89	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	0	84	dep	acid	59:62	arg1	status					104:109	acrosome status	95:109	acrosome status	95:109	Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status.					
32506705	3	85	theme	fertilization	581:593	arg1	medium					595:600	the fertilization medium	577:600	the fertilization medium	577:600	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	8	86	from	number	1560:1565	arg1	parameters					1616:1625	motility parameters	1607:1625	motility parameters	1607:1625	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	6	87	theme	fertilization	1278:1290	arg1	medium					1292:1297	the fertilization medium	1274:1297	the fertilization medium	1274:1297	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	6	88	from	pronounced	1365:1374	arg1	group					1330:1334	the 6,000 µg/ml group	1314:1334	the 6,000 µg/ml group in which the effect was more pronounced	1314:1374	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32506705	3	89	theme	HA	621:622	arg1	addition					609:616	the addition	605:616	the addition of HA	605:622	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	8	90	theme	lower	1521:1525	arg1	doses					1527:1531	lower doses	1521:1531	lower doses	1521:1531	However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa.					
32506705	5	91	theme	membrane	1042:1049	arg1	integrities					1051:1061	acrosomal membrane integrities	1032:1061	acrosomal membrane integrities	1032:1061	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	3	92	dep	determine	632:640	arg1	b					629:629	b	629:629	b	629:629	This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status.					
32506705	2	93	theme	Hyaluronic	339:348	arg1	glycosaminoglycan					365:381	a glycosaminoglycan	363:381	a glycosaminoglycan present in these fluids	363:405	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	2	93	theme	Hyaluronic	339:348	arg1	HA					356:357	HA	356:357	HA	356:357	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	2	93	theme	Hyaluronic	339:348	arg1	acid					350:353	Hyaluronic acid	339:353	Hyaluronic acid (HA)	339:358	Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood.					
32506705	5	94	theme	thawed	884:889	arg1	semen					891:895	thawed semen	884:895	thawed semen from 6 bulls	884:908	Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility).					
32506705	6	95	theme	6,000 µg/ml	1318:1328	arg1	group					1330:1334	the 6,000 µg/ml group	1314:1334	the 6,000 µg/ml group in which the effect was more pronounced	1314:1374	The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 µg/ml group in which the effect was more pronounced.					
32776872	0	0	theme	bone	87:90	arg1	mass					92:95	bone mass	87:95	bone mass	87:95	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	3	1	theme	calcium	576:582	arg1	levels					599:604	serum and urinary calcium and phosphorus levels	558:604	serum and urinary calcium and phosphorus levels	558:604	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	2	2	theme	sham	500:503	arg1	composition					448:458	body composition	443:458	body composition	443:458	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	2	theme	sham	500:503	arg1	metabolism					472:481	energy metabolism	465:481	energy metabolism	465:481	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	2	theme	sham	500:503	arg1	weight					435:440	body weight	430:440	body weight	430:440	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	1	3	theme	preclinical	151:161	arg1	studies					163:169	preclinical studies	151:169	preclinical studies	151:169	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	6	4	contain	have	977:980	arg1	butyrate					950:957	butyrate	950:957	butyrate	950:957	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	6	4	contain	have	977:980	arg1	acid					944:947	a short-chain fatty acid	924:947	a short-chain fatty acid	924:947	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	6	4	contain	have	977:980	arg2	effect					993:998	osteogenic effect	982:998	osteogenic effect	982:998	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	3	5	theme	serum	558:562	arg1	calcium					576:582	serum and urinary calcium	558:582	calcium	576:582	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	1	6	from	effects	375:381	arg1	rats					386:389	rats	386:389	rats	386:389	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	5	7	contain	had	849:851	arg1	treatment					791:799	scFOS treatment	785:799	scFOS treatment	785:799	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	5	7	contain	had	849:851	arg2	effect					856:861	no effect	853:861	no effect	853:861	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	1	8	theme	peak	253:256	arg1	PBM					269:271	PBM	269:271	PBM	269:271	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	1	8	theme	peak	253:256	arg1	mass					263:266	peak bone mass	253:266	peak bone mass (PBM)	253:272	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	6	9	theme	acid	944:947	arg1	levels					914:919	serum levels	908:919	serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect	908:998	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	2	10	theme	intact	492:497	arg1	sham					500:503	ovary intact (sham) and ovariectomized (OVX) rats	486:534	sham	500:503	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	11	theme	rats	531:534	arg1	composition					448:458	body composition	443:458	body composition	443:458	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	11	theme	rats	531:534	arg1	metabolism					472:481	energy metabolism	465:481	energy metabolism	465:481	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	11	theme	rats	531:534	arg1	weight					435:440	body weight	430:440	body weight	430:440	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	5	12	theme	higher	815:820	arg1	response					836:843	a higher bone anabolic response	813:843	a higher bone anabolic response	813:843	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	1	13	theme	bone	258:261	arg1	PBM					269:271	PBM	269:271	PBM	269:271	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	1	13	theme	bone	258:261	arg1	mass					263:266	peak bone mass	253:266	peak bone mass (PBM)	253:272	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	2	14	theme	body	430:433	arg1	weight					435:440	body weight	430:440	body weight	430:440	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	15	theme	ovary	486:490	arg1	sham					500:503	ovary intact (sham) and ovariectomized (OVX) rats	486:534	sham	500:503	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	7	16	theme	human	1069:1073	arg1	dose					1086:1089	the human equivalent dose	1065:1089	the human equivalent dose	1065:1089	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	5	17	theme	bone	822:825	arg1	response					836:843	a higher bone anabolic response	813:843	a higher bone anabolic response	813:843	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	3	18	theme	sham	680:683	arg1	group					685:689	the sham group	676:689	the sham group	676:689	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	7	19	theme	first	1019:1023	arg1	time					1025:1028	the first time	1015:1028	the first time	1015:1028	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	2	20	theme	OVX	526:528	arg1	rats					531:534	ovary intact (sham) and ovariectomized (OVX) rats	486:534	rats	531:534	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	5	21	theme	anabolic	827:834	arg1	response					836:843	a higher bone anabolic response	813:843	a higher bone anabolic response	813:843	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	6	22	theme	short-chain	926:936	arg1	butyrate					950:957	butyrate	950:957	butyrate	950:957	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	6	22	theme	short-chain	926:936	arg1	acid					944:947	a short-chain fatty acid	924:947	a short-chain fatty acid	924:947	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	0	23	theme	ovariectomized	100:113	arg1	rats					115:118	ovariectomized rats	100:118	ovariectomized rats	100:118	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	2	24	theme	ovariectomized	510:523	arg1	rats					531:534	ovary intact (sham) and ovariectomized (OVX) rats	486:534	rats	531:534	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	0	25	theme	prebiotic	2:10	arg1	fructo-oligosaccharides					25:47	A prebiotic, short-chain fructo-oligosaccharides	0:47	A prebiotic, short-chain fructo-oligosaccharides	0:47	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	3	26	theme	phosphorus	588:597	arg1	levels					599:604	serum and urinary calcium and phosphorus levels	558:604	serum and urinary calcium and phosphorus levels	558:604	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	4	27	theme	skeletal	733:740	arg1	parameters					742:751	better skeletal parameters	726:751	better skeletal parameters	726:751	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
32776872	3	28	theme	calcium	614:620	arg1	absorption					622:631	calcium absorption	614:631	calcium absorption	614:631	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	4	29	theme	OVX	701:703	arg1	rats					705:708	Sham and OVX rats	692:708	Sham and OVX rats given scFOS	692:720	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
32776872	7	30	from	dose	1086:1089	arg1	scFOS					1056:1060	scFOS	1056:1060	scFOS at the human equivalent dose	1056:1089	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	6	31	theme	osteogenic	982:991	arg1	effect					993:998	osteogenic effect	982:998	osteogenic effect	982:998	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	6	32	theme	serum	908:912	arg1	levels					914:919	serum levels	908:919	serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect	908:998	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	4	33	theme	respective	764:773	arg1	controls					775:782	their respective controls	758:782	their respective controls	758:782	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
32776872	6	34	theme	fatty	938:942	arg1	butyrate					950:957	butyrate	950:957	butyrate	950:957	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	6	34	theme	fatty	938:942	arg1	acid					944:947	a short-chain fatty acid	924:947	a short-chain fatty acid	924:947	scFOS increased serum levels of a short-chain fatty acid, butyrate which is known to have osteogenic effect.					
32776872	7	35	theme	bone	1147:1150	arg1	loss					1152:1155	E2 deficiency-induced bone loss	1125:1155	E2 deficiency-induced bone loss	1125:1155	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	2	36	theme	body	443:446	arg1	composition					448:458	body composition	443:458	body composition	443:458	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	1	37	dep	effect	243:248	arg1	studied					336:342	studied	336:342	has not been studied	323:342	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	1	38	theme	beneficial	207:216	arg1	effects					227:233	beneficial skeletal effects	207:233	beneficial skeletal effects	207:233	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	7	39	theme	selective	1160:1168	arg1	enhancement					1170:1180	selective enhancement	1160:1180	selective enhancement of new bone formation	1160:1202	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	1	40	theme	bone	278:281	arg1	loss					283:286	bone loss	278:286	bone loss	278:286	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	0	41	theme	osteogenic	126:135	arg1	mechanism					137:145	an osteogenic mechanism	123:145	an osteogenic mechanism	123:145	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	1	42	theme	skeletal	218:225	arg1	effects					227:233	beneficial skeletal effects	207:233	beneficial skeletal effects	207:233	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	0	43	theme	bone	63:66	arg1	mass					68:71	peak bone mass	58:71	peak bone mass	58:71	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	3	44	theme	increasing	646:655	arg1	trend					657:661	an increasing trend	643:661	an increasing trend	643:661	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	5	45	theme	catabolic	870:878	arg1	parameters					880:889	the catabolic parameters	866:889	the catabolic parameters	866:889	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	0	46	theme	peak	58:61	arg1	mass					68:71	peak bone mass	58:71	peak bone mass	58:71	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	1	47	from	effect	243:248	arg1	loss					283:286	bone loss	278:286	bone loss	278:286	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	1	47	from	effect	243:248	arg1	PBM					269:271	PBM	269:271	PBM	269:271	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	1	47	from	effect	243:248	arg1	mass					263:266	peak bone mass	253:266	peak bone mass (PBM)	253:272	In preclinical studies, fructooligosaccharide (FOS) showed beneficial skeletal effects but its effect on peak bone mass (PBM) and bone loss caused by estrogen (E2) deficiency has not been studied, and we set out to study these effects in rats.					
32776872	4	48	theme	Sham	692:695	arg1	rats					705:708	Sham and OVX rats	692:708	Sham and OVX rats given scFOS	692:720	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
32776872	2	49	contain	had	413:415	arg1	-FOS					408:411	Short-chain (sc)-FOS	392:411	Short-chain (sc)-FOS	392:411	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	2	49	contain	had	413:415	arg2	effect					420:425	no effect	417:425	no effect	417:425	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	4	50	theme	better	726:731	arg1	parameters					742:751	better skeletal parameters	726:751	better skeletal parameters	726:751	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
32776872	2	51	theme	energy	465:470	arg1	metabolism					472:481	energy metabolism	465:481	energy metabolism	465:481	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	7	52	theme	bone	1189:1192	arg1	formation					1194:1202	new bone formation	1185:1202	new bone formation	1185:1202	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	7	53	theme	formation	1194:1202	arg1	enhancement					1170:1180	selective enhancement	1160:1180	selective enhancement of new bone formation	1160:1202	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	5	54	theme	scFOS	785:789	arg1	treatment					791:799	scFOS treatment	785:799	scFOS treatment	785:799	scFOS treatment resulted in a higher bone anabolic response but had no effect on the catabolic parameters.					
32776872	7	55	theme	E2	1125:1126	arg1	loss					1152:1155	E2 deficiency-induced bone loss	1125:1155	E2 deficiency-induced bone loss	1125:1155	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	0	56	dep	prebiotic	2:10	arg1	short-chain					13:23	short-chain	13:23	short-chain	13:23	A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism.					
32776872	7	57	theme	deficiency-induced	1128:1145	arg1	loss					1152:1155	E2 deficiency-induced bone loss	1125:1155	E2 deficiency-induced bone loss	1125:1155	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	7	58	theme	equivalent	1075:1084	arg1	dose					1086:1089	the human equivalent dose	1065:1089	the human equivalent dose	1065:1089	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	2	59	theme	Short-chain	392:402	arg1	-FOS					408:411	Short-chain (sc)-FOS	392:411	Short-chain (sc)-FOS	392:411	Short-chain (sc)-FOS had no effect on body weight, body composition, and energy metabolism of ovary intact (sham) and ovariectomized (OVX) rats.					
32776872	3	60	theme	urinary	568:574	arg1	calcium					576:582	serum and urinary calcium	558:582	calcium	576:582	scFOS did not affect serum and urinary calcium and phosphorus levels, and on calcium absorption, although an increasing trend was noted in the sham group.					
32776872	7	61	theme	new	1185:1187	arg1	formation					1194:1202	new bone formation	1185:1202	new bone formation	1185:1202	Our study for the first time demonstrates that in rats scFOS at the human equivalent dose enhances PBM and protects against E2 deficiency-induced bone loss by selective enhancement of new bone formation, and implicates butyrate in this process.					
32776872	4	62	contain	had	722:724	arg1	rats					705:708	Sham and OVX rats	692:708	Sham and OVX rats given scFOS	692:720	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
32776872	4	62	contain	had	722:724	arg2	parameters					742:751	better skeletal parameters	726:751	better skeletal parameters	726:751	Sham and OVX rats given scFOS had better skeletal parameters than their respective controls.					
34170952	2	0	with	release	380:386	arg1	formulation					414:424	an optimum retentive formulation	393:424	an optimum retentive formulation in the upper stomach	393:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	4	1	theme	gas	839:841	arg1	agent					854:858	gas generating agent	839:858	gas generating agent	839:858	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	7	2	theme	h	1519:1519	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	2	theme	h	1519:1519	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	3	from	%	1509:1509	arg1	h					1503:1503	12 h	1500:1503	12 h	1500:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	6	4	theme	drug	1276:1279	arg1	release					1281:1287	50% drug release	1272:1287	50% drug release (t50%) (Y5)	1272:1299	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	4	theme	drug	1276:1279	arg1	%					1293:1293	t50%	1290:1293	t50%	1290:1293	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	4	theme	drug	1276:1279	arg1	Y5					1297:1298	Y5	1297:1298	Y5	1297:1298	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	5	theme	bioadhesive	1171:1181	arg1	strength					1183:1190	bioadhesive strength	1171:1190	bioadhesive strength (Y2)	1171:1195	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	5	theme	bioadhesive	1171:1181	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	5	theme	bioadhesive	1171:1181	arg1	Y2					1193:1194	Y2	1193:1194	Y2	1193:1194	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	1	6	theme	absorption	277:286	arg1	window					288:293	narrow absorption window	270:293	narrow absorption window	270:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	6	7	theme	cumulative	1305:1314	arg1	release					1321:1327	cumulative drug release	1305:1327	cumulative drug release at 12 h (Y6)	1305:1340	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	1	8	theme	H2-receptor	188:198	arg1	HCl					148:150	Ranitidine HCl	137:150	Ranitidine HCl	137:150	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	8	theme	H2-receptor	188:198	arg1	antagonist					200:209	a selective, competitive histamine H2-receptor antagonist	153:209	a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window	153:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	7	9	theme	floating	1407:1414	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	10	theme	ranitidine	1899:1908	arg1	tablet					1914:1919	the conventional ranitidine HCl tablet	1882:1919	the conventional ranitidine HCl tablet	1882:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	0	11	theme	ranitidine	111:120	arg1	hydrochloride					122:134	ranitidine hydrochloride	111:134	ranitidine hydrochloride	111:134	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	5	12	theme	formulations	1020:1031	arg1	total					1002:1006	a total	1000:1006	a total of thirteen formulations	1000:1031	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	4	13	theme	direct	900:905	arg1	diluent					919:925	direct compression diluent	900:925	direct compression diluent	900:925	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	3	14	theme	bioadhesive	580:590	arg1	tablets					614:620	floating, bioadhesive, and swellable matrix tablets	570:620	floating, bioadhesive, and swellable matrix tablets of ranitidine HCl	570:638	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	1	15	theme	short	218:222	arg1	half-life					235:243	a short biological half-life	216:243	a short biological half-life	216:243	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	4	16	theme	microcrystalline	864:879	arg1	cellulose					881:889	microcrystalline cellulose	864:889	microcrystalline cellulose (MCC)	864:895	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	16	theme	microcrystalline	864:879	arg1	MCC					892:894	MCC	892:894	MCC	892:894	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	6	17	theme	floating	1147:1154	arg1	Y1					1166:1167	Y1	1166:1167	Y1	1166:1167	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	17	theme	floating	1147:1154	arg1	release					1243:1249	cumulative drug release	1227:1249	cumulative drug release at 1 h (Y4)	1227:1261	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	17	theme	floating	1147:1154	arg1	time					1264:1267	time	1264:1267	time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6)	1264:1340	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	17	theme	floating	1147:1154	arg1	index					1207:1211	swelling index	1198:1211	swelling index at 12 h (Y3)	1198:1224	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	17	theme	floating	1147:1154	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	17	theme	floating	1147:1154	arg1	strength					1183:1190	bioadhesive strength	1171:1190	bioadhesive strength (Y2)	1171:1195	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	17	theme	floating	1147:1154	arg1	variables					1369:1377	the response variables	1356:1377	the response variables	1356:1377	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	18	theme	swelling	1198:1205	arg1	index					1207:1211	swelling index	1198:1211	swelling index at 12 h (Y3)	1198:1224	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	18	theme	swelling	1198:1205	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	3	19	theme	floating	570:577	arg1	tablets					614:620	floating, bioadhesive, and swellable matrix tablets	570:620	floating, bioadhesive, and swellable matrix tablets of ranitidine HCl	570:638	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	4	20	theme	methylcellulose	711:725	arg1	bicarbonate					815:825	sodium bicarbonate	808:825	sodium bicarbonate (NaHCO3)	808:834	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	20	theme	methylcellulose	711:725	arg1	combination					682:692	a combination	680:692	a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers	680:805	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	3	21	theme	matrix	607:612	arg1	tablets					614:620	floating, bioadhesive, and swellable matrix tablets	570:620	floating, bioadhesive, and swellable matrix tablets of ranitidine HCl	570:638	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	1	22	theme	Ranitidine	137:146	arg1	candidate					308:316	an ideal candidate	299:316	an ideal candidate for gastro-retentive drug delivery system (GRDDS)	299:366	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	22	theme	Ranitidine	137:146	arg1	antagonist					200:209	a selective, competitive histamine H2-receptor antagonist	153:209	a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window	153:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	22	theme	Ranitidine	137:146	arg1	HCl					148:150	Ranitidine HCl	137:150	Ranitidine HCl	137:150	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	7	23	theme	swelling	1471:1478	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	6	24	theme	independent	1120:1130	arg1	variables					1132:1140	independent variables	1120:1140	independent variables	1120:1140	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	24	theme	independent	1120:1130	arg1	Concentration					1048:1060	Concentration	1048:1060	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2)	1048:1101	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	0	25	theme	floating	58:65	arg1	release					50:56	sustained release	40:56	sustained release floating, bioadhesive and swellable	40:92	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	1	26	theme	drug	339:342	arg1	system					353:358	gastro-retentive drug delivery system	322:358	gastro-retentive drug delivery system (GRDDS)	322:366	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	26	theme	drug	339:342	arg1	GRDDS					361:365	GRDDS	361:365	GRDDS	361:365	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	6	27	from	h	1335:1335	arg1	release					1281:1287	50% drug release	1272:1287	50% drug release (t50%) (Y5)	1272:1299	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	27	from	h	1335:1335	arg1	release					1321:1327	cumulative drug release	1305:1327	cumulative drug release at 12 h (Y6)	1305:1340	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	27	from	h	1335:1335	arg1	Y5					1297:1298	Y5	1297:1298	Y5	1297:1298	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	27	from	h	1335:1335	arg1	%					1293:1293	t50%	1290:1293	t50%	1290:1293	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	7	28	theme	g	1468:1468	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	28	theme	g	1468:1468	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	0	29	theme	bioadhesive	68:78	arg1	release					50:56	sustained release	40:56	sustained release floating, bioadhesive and swellable	40:92	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	1	30	theme	selective	155:163	arg1	HCl					148:150	Ranitidine HCl	137:150	Ranitidine HCl	137:150	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	30	theme	selective	155:163	arg1	antagonist					200:209	a selective, competitive histamine H2-receptor antagonist	153:209	a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window	153:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	4	31	theme	carboxymethyl	745:757	arg1	cellulose					759:767	sodium carboxymethyl cellulose	738:767	sodium carboxymethyl cellulose	738:767	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	0	32	theme	swellable	84:92	arg1	release					50:56	sustained release	40:56	sustained release floating, bioadhesive and swellable	40:92	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	7	33	theme	release	1604:1610	arg1	mechanism					1612:1620	anomalous release mechanism	1594:1620	anomalous release mechanism	1594:1620	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	34	theme	bioadhesive	1438:1448	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	2	35	theme	optimum	396:402	arg1	formulation					414:424	an optimum retentive formulation	393:424	an optimum retentive formulation in the upper stomach	393:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	8	36	theme	matrix	1667:1672	arg1	strategy					1799:1806	a strategy	1797:1806	a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet	1797:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	8	36	theme	matrix	1667:1672	arg1	tablet					1674:1679	sustained release matrix tablet	1649:1679	sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties	1649:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	7	37	theme	sec	1433:1435	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	37	theme	sec	1433:1435	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	38	theme	gastro-retentive	1748:1763	arg1	properties					1765:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	8	39	theme	HCl	1695:1697	arg1	strategy					1799:1806	a strategy	1797:1806	a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet	1797:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	8	39	theme	HCl	1695:1697	arg1	tablet					1674:1679	sustained release matrix tablet	1649:1679	sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties	1649:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	5	40	theme	Central	928:934	arg1	design					946:951	Central composite design	928:951	Central composite design (CCD)	928:957	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	5	40	theme	Central	928:934	arg1	CCD					954:956	CCD	954:956	CCD	954:956	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	7	41	theme	lag	1416:1418	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	3	42	theme	HCl	636:638	arg1	tablets					614:620	floating, bioadhesive, and swellable matrix tablets	570:620	floating, bioadhesive, and swellable matrix tablets of ranitidine HCl	570:638	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	8	43	theme	combined	1704:1711	arg1	properties					1765:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	1	44	theme	ideal	302:306	arg1	candidate					308:316	an ideal candidate	299:316	an ideal candidate for gastro-retentive drug delivery system (GRDDS)	299:366	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	44	theme	ideal	302:306	arg1	HCl					148:150	Ranitidine HCl	137:150	Ranitidine HCl	137:150	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	2	45	theme	ideal	459:463	arg1	release					380:386	Controlled release	369:386	Controlled release with an optimum retentive formulation in the upper stomach	369:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	2	45	theme	ideal	459:463	arg1	formulation					465:475	an ideal formulation	456:475	an ideal formulation for this drug	456:489	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	4	46	theme	sodium	808:813	arg1	NaHCO3					828:833	NaHCO3	828:833	NaHCO3	828:833	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	46	theme	sodium	808:813	arg1	bicarbonate					815:825	sodium bicarbonate	808:825	sodium bicarbonate (NaHCO3)	808:834	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	46	theme	sodium	808:813	arg1	combination					682:692	a combination	680:692	a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers	680:805	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	8	47	with	HCl	1695:1697	arg1	properties					1765:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	0	48	theme	sustained	40:48	arg1	release					50:56	sustained release	40:56	sustained release floating, bioadhesive and swellable	40:92	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	1	49	theme	biological	224:233	arg1	half-life					235:243	a short biological half-life	216:243	a short biological half-life	216:243	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	2	50	from	formulation	414:424	arg1	stomach					439:445	the upper stomach	429:445	the upper stomach	429:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	7	51	from	h	1503:1503	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	51	from	h	1503:1503	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	52	dep	in	1848:1849	arg1	vivo					1851:1854	vivo	1851:1854	vivo	1851:1854	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	4	53	theme	release	780:786	arg1	polymers					798:805	release retarding polymers	780:805	release retarding polymers	780:805	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	2	54	theme	Controlled	369:378	arg1	release					380:386	Controlled release	369:386	Controlled release with an optimum retentive formulation in the upper stomach	369:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	2	54	theme	Controlled	369:378	arg1	formulation					465:475	an ideal formulation	456:475	an ideal formulation for this drug	456:489	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	8	55	theme	bioadhesive	1723:1733	arg1	properties					1765:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	0	56	dep	Design	0:5	arg1	tablet					101:106	matrix tablet	94:106	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.	0:135	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	6	57	theme	HPMC/NaCMC	1065:1074	arg1	variables					1132:1140	independent variables	1120:1140	independent variables	1120:1140	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	57	theme	HPMC/NaCMC	1065:1074	arg1	Concentration					1048:1060	Concentration	1048:1060	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2)	1048:1101	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	8	58	theme	release	1659:1665	arg1	strategy					1799:1806	a strategy	1797:1806	a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet	1797:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	8	58	theme	release	1659:1665	arg1	tablet					1674:1679	sustained release matrix tablet	1649:1679	sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties	1649:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	6	59	theme	%	1274:1274	arg1	release					1281:1287	50% drug release	1272:1287	50% drug release (t50%) (Y5)	1272:1299	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	59	theme	%	1274:1274	arg1	%					1293:1293	t50%	1290:1293	t50%	1290:1293	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	59	theme	%	1274:1274	arg1	Y5					1297:1298	Y5	1297:1298	Y5	1297:1298	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	1	60	theme	narrow	270:275	arg1	window					288:293	narrow absorption window	270:293	narrow absorption window	270:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	4	61	theme	generating	843:852	arg1	agent					854:858	gas generating agent	839:858	gas generating agent	839:858	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	8	62	theme	in	1848:1849	arg1	variation					1856:1864	in vivo variation	1848:1864	in vivo variation	1848:1864	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	3	63	theme	study	515:519	arg1	aim					496:498	The aim	492:498	The aim of the present study	492:519	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	6	64	theme	drug	1238:1241	arg1	release					1243:1249	cumulative drug release	1227:1249	cumulative drug release at 1 h (Y4)	1227:1261	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	64	theme	drug	1238:1241	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	65	theme	drug	1316:1319	arg1	release					1321:1327	cumulative drug release	1305:1327	cumulative drug release at 12 h (Y6)	1305:1340	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	1	66	theme	histamine	178:186	arg1	HCl					148:150	Ranitidine HCl	137:150	Ranitidine HCl	137:150	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	66	theme	histamine	178:186	arg1	antagonist					200:209	a selective, competitive histamine H2-receptor antagonist	153:209	a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window	153:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	7	67	theme	%	1495:1495	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	67	theme	%	1495:1495	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	6	68	theme	NaHCO3	1091:1096	arg1	variables					1132:1140	independent variables	1120:1140	independent variables	1120:1140	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	68	theme	NaHCO3	1091:1096	arg1	Concentration					1048:1060	Concentration	1048:1060	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2)	1048:1101	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	4	69	theme	matrix	645:650	arg1	tablets					652:658	The matrix tablets	641:658	The matrix tablets	641:658	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	0	70	theme	hydrochloride	122:134	arg1	tablet					101:106	matrix tablet	94:106	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.	0:135	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	7	71	from	index	1480:1484	arg1	h					1503:1503	12 h	1500:1503	12 h	1500:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	72	theme	optimized	1384:1392	arg1	batch					1394:1398	The optimized batch	1380:1398	The optimized batch	1380:1398	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	73	theme	conventional	1886:1897	arg1	tablet					1914:1919	the conventional ranitidine HCl tablet	1882:1919	the conventional ranitidine HCl tablet	1882:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	6	74	theme	lag	1156:1158	arg1	Y1					1166:1167	Y1	1166:1167	Y1	1166:1167	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	74	theme	lag	1156:1158	arg1	release					1243:1249	cumulative drug release	1227:1249	cumulative drug release at 1 h (Y4)	1227:1261	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	74	theme	lag	1156:1158	arg1	time					1264:1267	time	1264:1267	time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6)	1264:1340	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	74	theme	lag	1156:1158	arg1	index					1207:1211	swelling index	1198:1211	swelling index at 12 h (Y3)	1198:1224	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	74	theme	lag	1156:1158	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	74	theme	lag	1156:1158	arg1	strength					1183:1190	bioadhesive strength	1171:1190	bioadhesive strength (Y2)	1171:1195	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	74	theme	lag	1156:1158	arg1	variables					1369:1377	the response variables	1356:1377	the response variables	1356:1377	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	75	theme	cumulative	1227:1236	arg1	release					1243:1249	cumulative drug release	1227:1249	cumulative drug release at 1 h (Y4)	1227:1261	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	75	theme	cumulative	1227:1236	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	76	from	h	1256:1256	arg1	release					1243:1249	cumulative drug release	1227:1249	cumulative drug release at 1 h (Y4)	1227:1261	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	76	from	h	1256:1256	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	3	77	theme	swellable	597:605	arg1	tablets					614:620	floating, bioadhesive, and swellable matrix tablets	570:620	floating, bioadhesive, and swellable matrix tablets of ranitidine HCl	570:638	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	4	78	theme	hydroxypropyl	697:709	arg1	NaCMC					770:774	NaCMC	770:774	NaCMC	770:774	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	78	theme	hydroxypropyl	697:709	arg1	HPMC					728:731	HPMC	728:731	HPMC	728:731	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	78	theme	hydroxypropyl	697:709	arg1	methylcellulose					711:725	hydroxypropyl methylcellulose	697:725	hydroxypropyl methylcellulose (HPMC)	697:732	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	7	79	from	strength	1450:1457	arg1	h					1503:1503	12 h	1500:1503	12 h	1500:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	80	theme	sustained	1649:1657	arg1	strategy					1799:1806	a strategy	1797:1806	a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet	1797:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	8	80	theme	sustained	1649:1657	arg1	tablet					1674:1679	sustained release matrix tablet	1649:1679	sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties	1649:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	1	81	theme	delivery	344:351	arg1	system					353:358	gastro-retentive drug delivery system	322:358	gastro-retentive drug delivery system (GRDDS)	322:366	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	81	theme	delivery	344:351	arg1	GRDDS					361:365	GRDDS	361:365	GRDDS	361:365	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	0	82	theme	matrix	94:99	arg1	tablet					101:106	matrix tablet	94:106	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.	0:135	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	7	83	theme	anomalous	1594:1602	arg1	release					1604:1610	anomalous release	1594:1610	anomalous release mechanism	1594:1620	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	5	84	theme	composite	936:944	arg1	design					946:951	Central composite design	928:951	Central composite design (CCD)	928:957	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	5	84	theme	composite	936:944	arg1	CCD					954:956	CCD	954:956	CCD	954:956	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	4	85	theme	compression	907:917	arg1	diluent					919:925	direct compression diluent	900:925	direct compression diluent	900:925	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	86	theme	sodium	738:743	arg1	cellulose					759:767	sodium carboxymethyl cellulose	738:767	sodium carboxymethyl cellulose	738:767	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	8	87	theme	swelling	1739:1746	arg1	properties					1765:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	4	88	theme	cellulose	759:767	arg1	bicarbonate					815:825	sodium bicarbonate	808:825	sodium bicarbonate (NaHCO3)	808:834	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	4	88	theme	cellulose	759:767	arg1	combination					682:692	a combination	680:692	a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers	680:805	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	8	89	theme	ranitidine	1684:1693	arg1	HCl					1695:1697	ranitidine HCl	1684:1697	ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties	1684:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	1	90	dep	selective	155:163	arg1	competitive					166:176	competitive	166:176	competitive	166:176	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	6	91	theme	response	1360:1367	arg1	variables					1369:1377	the response variables	1356:1377	the response variables	1356:1377	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	91	theme	response	1360:1367	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	7	92	from	release	1530:1536	arg1	h					1503:1503	12 h	1500:1503	12 h	1500:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	2	93	theme	upper	433:437	arg1	stomach					439:445	the upper stomach	429:445	the upper stomach	429:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	3	94	theme	ranitidine	625:634	arg1	HCl					636:638	ranitidine HCl	625:638	ranitidine HCl	625:638	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	8	95	theme	floating	1713:1720	arg1	properties					1765:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	combined floating, bioadhesive and swelling gastro-retentive properties	1704:1774	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	6	96	from	h	1219:1219	arg1	index					1207:1211	swelling index	1198:1211	swelling index at 12 h (Y3)	1198:1224	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	6	96	from	h	1219:1219	arg1	time					1160:1163	floating lag time	1147:1163	floating lag time (Y1)	1147:1168	Concentration of HPMC/NaCMC (3:1) (X1) and NaHCO3 (X2) were selected as independent variables; and floating lag time (Y1), bioadhesive strength (Y2), swelling index at 12 h (Y3), cumulative drug release at 1 h (Y4), time to 50% drug release (t50%) (Y5) and cumulative drug release at 12 h (Y6) were taken as the response variables.					
34170952	1	97	with	antagonist	200:209	arg1	window					288:293	narrow absorption window	270:293	narrow absorption window	270:293	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	97	with	antagonist	200:209	arg1	half-life					235:243	a short biological half-life	216:243	a short biological half-life	216:243	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	97	with	antagonist	200:209	arg1	bioavailability					250:264	low bioavailability	246:264	low bioavailability	246:264	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	4	98	theme	retarding	788:796	arg1	polymers					798:805	release retarding polymers	780:805	release retarding polymers	780:805	The matrix tablets were prepared using a combination of hydroxypropyl methylcellulose (HPMC) and sodium carboxymethyl cellulose (NaCMC) as release retarding polymers, sodium bicarbonate (NaHCO3) as gas generating agent and microcrystalline cellulose (MCC) as direct compression diluent.					
34170952	2	99	theme	retentive	404:412	arg1	formulation					414:424	an optimum retentive formulation	393:424	an optimum retentive formulation in the upper stomach	393:445	Controlled release with an optimum retentive formulation in the upper stomach would be an ideal formulation for this drug.					
34170952	0	100	theme	release	50:56	arg1	development					8:18	development	8:18	development	8:18	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	0	100	theme	release	50:56	arg1	Design					0:5	Design	0:5	Design	0:5	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	0	100	theme	release	50:56	arg1	optimization					24:35	optimization	24:35	optimization	24:35	Design, development and optimization of sustained release floating, bioadhesive and swellable matrix tablet of ranitidine hydrochloride.					
34170952	7	101	theme	%	1557:1557	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	101	theme	%	1557:1557	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	index					1480:1484	swelling index	1471:1484	swelling index of 315.04% at 12 h	1471:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	time					1420:1423	floating lag time	1407:1423	floating lag time of 5.09 sec	1407:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	%					1557:1557	93.65%	1552:1557	93.65%	1552:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	%					1509:1509	t50%	1506:1509	t50% of 3.86 h	1506:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	g					1468:1468	29.69 g	1462:1468	29.69 g	1462:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	%					1495:1495	315.04%	1489:1495	315.04% at 12 h	1489:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	sec					1433:1435	5.09 sec	1428:1435	5.09 sec	1428:1435	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	%					1546:1546	24.21%	1541:1546	24.21%	1541:1546	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	h					1519:1519	3.86 h	1514:1519	3.86 h	1514:1519	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	102	theme	%	1546:1546	arg1	strength					1450:1457	bioadhesive strength	1438:1457	bioadhesive strength of 29.69 g	1438:1468	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	103	theme	HCl	1910:1912	arg1	tablet					1914:1919	the conventional ranitidine HCl tablet	1882:1919	the conventional ranitidine HCl tablet	1882:1919	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	5	104	used	used	963:966	arg2	CCD					954:956	CCD	954:956	CCD	954:956	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	5	104	used	used	963:966	arg2	design					946:951	Central composite design	928:951	Central composite design (CCD)	928:957	Central composite design (CCD) was used to optimize the formulation and a total of thirteen formulations were prepared.					
34170952	1	105	theme	gastro-retentive	322:337	arg1	system					353:358	gastro-retentive drug delivery system	322:358	gastro-retentive drug delivery system (GRDDS)	322:366	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	1	105	theme	gastro-retentive	322:337	arg1	GRDDS					361:365	GRDDS	361:365	GRDDS	361:365	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
34170952	7	106	from	time	1420:1423	arg1	h					1503:1503	12 h	1500:1503	12 h	1500:1503	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	7	107	theme	drug	1525:1528	arg1	release					1530:1536	drug release	1525:1536	drug release of 24.21% and 93.65%	1525:1557	The optimized batch showed floating lag time of 5.09 sec, bioadhesive strength of 29.69 g, swelling index of 315.04% at 12 h, t50% of 3.86 h and drug release of 24.21% and 93.65% at 1h and 12 h, respectively, with anomalous release mechanism.					
34170952	8	108	theme	low	1824:1826	arg1	bioavailability					1828:1842	the low bioavailability	1820:1842	the low bioavailability	1820:1842	The results indicate that sustained release matrix tablet of ranitidine HCl with combined floating, bioadhesive and swelling gastro-retentive properties can be considered as a strategy to overcome the low bioavailability and in vivo variation associated with the conventional ranitidine HCl tablet.					
34170952	3	109	theme	present	507:513	arg1	study					515:519	the present study	503:519	the present study	503:519	The aim of the present study was therefore to develop, formulate and optimize floating, bioadhesive, and swellable matrix tablets of ranitidine HCl.					
34170952	1	110	theme	low	246:248	arg1	bioavailability					250:264	low bioavailability	246:264	low bioavailability	246:264	Ranitidine HCl, a selective, competitive histamine H2-receptor antagonist with a short biological half-life, low bioavailability and narrow absorption window, is an ideal candidate for gastro-retentive drug delivery system (GRDDS).					
32168415	0	0	theme	gut	37:39	arg1	microbiota					41:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	12	1	theme	Gut	1721:1723	arg1	alterations					1735:1745	Gut microbial alterations	1721:1745	Gut microbial alterations induced increase in Gram-positive bacterial LTA	1721:1793	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	6	2	theme	duodenal	1001:1008	arg1	externa					1021:1027	duodenal muscularis externa	1001:1027	duodenal muscularis externa contractions	1001:1040	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	1	3	theme	intestinal	270:279	arg1	dysmotility					281:291	intestinal dysmotility	270:291	intestinal dysmotility	270:291	BACKGROUND High-fat diet, microbial alterations and lipopolysaccharide (LPS) are thought to cause enteric diabetic neuropathy and intestinal dysmotility.					
32168415	10	4	theme	oxidative	1563:1571	arg1	stress					1573:1578	increased oxidative stress	1553:1578	increased oxidative stress	1553:1578	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	10	5	from	density	1510:1516	arg1	ganglia					1613:1619	myenteric ganglia	1603:1619	myenteric ganglia	1603:1619	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	10	6	theme	contractions	1454:1465	arg1	frequency					1421:1429	the frequency	1417:1429	the frequency of duodenal muscularis contractions after 24 hours of cultured	1417:1492	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	7	7	theme	diet	1114:1117	arg1	ingestion					1119:1127	High-fat diet ingestion	1105:1127	High-fat diet ingestion	1105:1127	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	2	8	theme	gut	319:321	arg1	acid					348:351	lipoteichoic acid	335:351	lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs)	335:421	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	8	theme	gut	319:321	arg1	microbiota					323:332	the gut microbiota	315:332	the gut microbiota	315:332	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	9	9	theme	increased	1343:1351	arg1	abundance					1353:1361	increased abundance	1343:1361	increased abundance of Gram-positive bacteria	1343:1387	CR mice fed a HFD had increased abundance of Gram-positive bacteria.					
32168415	6	10	theme	cultured	1070:1077	arg1	preparations					1079:1090	cultured preparations	1070:1090	cultured preparations	1070:1090	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	10	11	theme	cultured	1485:1492	arg1	24 hours					1473:1480	24 hours	1473:1480	24 hours of cultured	1473:1492	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	0	12	theme	neuropathy	128:137	arg1	development					105:115	the development	101:115	the development of enteric neuropathy	101:137	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	10	13	theme	nitrergic	1521:1529	arg1	neurons					1541:1547	nitrergic myenteric neurons	1521:1547	nitrergic myenteric neurons	1521:1547	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	9	14	theme	CR	1321:1322	arg1	mice					1324:1327	CR mice	1321:1327	CR mice	1321:1327	CR mice fed a HFD had increased abundance of Gram-positive bacteria.					
32168415	9	15	theme	bacteria	1380:1387	arg1	abundance					1353:1361	increased abundance	1343:1361	increased abundance of Gram-positive bacteria	1343:1387	CR mice fed a HFD had increased abundance of Gram-positive bacteria.					
32168415	6	16	theme	externa	1021:1027	arg1	contractions					1029:1040	duodenal muscularis externa contractions	1001:1040	duodenal muscularis externa contractions	1001:1040	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	9	17	dep	fed	1329:1331	arg1	had					1339:1341	had	1339:1341	fed a HFD had increased abundance of Gram-positive bacteria	1329:1387	CR mice fed a HFD had increased abundance of Gram-positive bacteria.					
32168415	3	18	from	role	551:554	arg1	development					600:610	the development	596:610	the development of diabetic enteric neuropathy and intestinal dysmotility	596:668	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	1	19	theme	High-fat	151:158	arg1	diet					160:163	BACKGROUND High-fat diet	140:163	BACKGROUND High-fat diet	140:163	BACKGROUND High-fat diet, microbial alterations and lipopolysaccharide (LPS) are thought to cause enteric diabetic neuropathy and intestinal dysmotility.					
32168415	2	20	from	role	307:310	arg1	development					430:440	the development	426:440	the development of diabetic enteric neuropathy	426:471	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	20	from	role	307:310	arg1	dysmotility					488:498	intestinal dysmotility	477:498	intestinal dysmotility	477:498	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	1	21	theme	BACKGROUND	140:149	arg1	diet					160:163	BACKGROUND High-fat diet	140:163	BACKGROUND High-fat diet	140:163	BACKGROUND High-fat diet, microbial alterations and lipopolysaccharide (LPS) are thought to cause enteric diabetic neuropathy and intestinal dysmotility.					
32168415	11	22	theme	muscularis	1643:1652	arg1	contractions					1654:1665	muscularis contractions	1643:1665	muscularis contractions	1643:1665	SCFAs did not affect muscularis contractions or injure myenteric neurons.					
32168415	0	23	theme	lipoteichoic	68:79	arg1	acid					81:84	gut-derived lipoteichoic acid	56:84	gut-derived lipoteichoic acid	56:84	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	4	24	dep	mice	732:735	arg1	either					737:742	either	737:742	either	737:742	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	5	25	theme	community	828:836	arg1	composition					838:848	the microbial community composition	814:848	the microbial community composition in CR mice	814:859	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	8	26	theme	GF	1259:1260	arg1	mice					1262:1265	GF mice	1259:1265	GF mice	1259:1265	GF mice had fewer neurons per ganglion compared with CR mice.					
32168415	11	27	theme	myenteric	1677:1685	arg1	neurons					1687:1693	myenteric neurons	1677:1693	myenteric neurons	1677:1693	SCFAs did not affect muscularis contractions or injure myenteric neurons.					
32168415	7	28	theme	total	1141:1145	arg1	number					1147:1152	the total number	1137:1152	the total number	1137:1152	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	2	29	theme	fatty	403:407	arg1	SCFAs					416:420	SCFAs	416:420	SCFAs	416:420	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	29	theme	fatty	403:407	arg1	acids					409:413	short-chain fatty acids	391:413	short-chain fatty acids (SCFAs)	391:421	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	5	30	theme	myenteric	901:909	arg1	neurons					911:917	myenteric neurons	901:917	myenteric neurons using immunohistochemistry	901:944	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	0	31	theme	High-fat	0:7	arg1	microbiota					41:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	5	32	from	composition	838:848	arg1	mice					856:859	CR mice	853:859	CR mice	853:859	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	5	33	theme	16S	867:869	arg1	sequencing					876:885	16S rRNA sequencing	867:885	16S rRNA sequencing	867:885	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	6	34	theme	LTA	983:985	arg1	effects					967:973	the effects	963:973	the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons	963:1062	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	0	35	theme	alterations	22:32	arg1	microbiota					41:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	4	36	dep	germ-free	686:694	arg1	GF					697:698	GF	697:698	GF	697:698	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	4	36	dep	germ-free	686:694	arg1	CR					728:729	CR	728:729	CR	728:729	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	6	37	theme	LPS	978:980	arg1	effects					967:973	the effects	963:973	the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons	963:1062	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	3	38	theme	enteric	624:630	arg1	neuropathy					632:641	diabetic enteric neuropathy	615:641	diabetic enteric neuropathy	615:641	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	3	39	dep	gut	563:565	arg1	SCFAs					587:591	SCFAs	587:591	SCFAs	587:591	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	3	39	dep	gut	563:565	arg1	LTA					579:581	LTA	579:581	LTA	579:581	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	3	39	dep	gut	563:565	arg1	microbiota					567:576	microbiota	567:576	microbiota	567:576	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	2	40	theme	neuropathy	462:471	arg1	development					430:440	the development	426:440	the development of diabetic enteric neuropathy	426:471	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	40	theme	neuropathy	462:471	arg1	dysmotility					488:498	intestinal dysmotility	477:498	intestinal dysmotility	477:498	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	41	theme	enteric	454:460	arg1	neuropathy					462:471	diabetic enteric neuropathy	445:471	diabetic enteric neuropathy	445:471	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	4	42	theme	chow	773:776	arg1	SCD					784:786	SCD	784:786	SCD	784:786	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	4	42	theme	chow	773:776	arg1	diet					778:781	standard chow diet	764:781	standard chow diet (SCD)	764:787	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	12	43	theme	enteric	1813:1819	arg1	neuropathy					1821:1830	enteric neuropathy	1813:1830	enteric neuropathy	1813:1830	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	12	44	theme	Gram-positive	1767:1779	arg1	LTA					1791:1793	Gram-positive bacterial LTA	1767:1793	Gram-positive bacterial LTA	1767:1793	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	6	45	theme	myenteric	1046:1054	arg1	neurons					1056:1062	myenteric neurons	1046:1062	myenteric neurons	1046:1062	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	3	46	theme	dysmotility	658:668	arg1	development					600:610	the development	596:610	the development of diabetic enteric neuropathy and intestinal dysmotility	596:668	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	4	47	theme	high-fat	746:753	arg1	HFD					756:758	HFD	756:758	HFD	756:758	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	2	48	theme	lipoteichoic	335:346	arg1	acid					348:351	lipoteichoic acid	335:351	lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs)	335:421	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	48	theme	lipoteichoic	335:346	arg1	LTA					354:356	LTA	354:356	LTA	354:356	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	48	theme	lipoteichoic	335:346	arg1	microbiota					323:332	the gut microbiota	315:332	the gut microbiota	315:332	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	10	49	theme	muscularis	1443:1452	arg1	contractions					1454:1465	duodenal muscularis contractions	1434:1465	duodenal muscularis contractions after 24 hours of cultured	1434:1492	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	2	50	theme	Gram-positive	364:376	arg1	bacteria					378:385	Gram-positive bacteria	364:385	Gram-positive bacteria	364:385	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	1	51	theme	diabetic	246:253	arg1	neuropathy					255:264	enteric diabetic neuropathy	238:264	enteric diabetic neuropathy	238:264	BACKGROUND High-fat diet, microbial alterations and lipopolysaccharide (LPS) are thought to cause enteric diabetic neuropathy and intestinal dysmotility.					
32168415	10	52	from	production	1589:1598	arg1	ganglia					1613:1619	myenteric ganglia	1603:1619	myenteric ganglia	1603:1619	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	7	53	theme	neurons	1192:1198	arg1	number					1147:1152	the total number	1137:1152	the total number	1137:1152	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	7	53	theme	neurons	1192:1198	arg1	number					1162:1167	the number	1158:1167	the number of nitrergic myenteric neurons per ganglion	1158:1211	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	7	54	theme	nitrergic	1172:1180	arg1	neurons					1192:1198	nitrergic myenteric neurons	1172:1198	nitrergic myenteric neurons	1172:1198	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	5	55	theme	microbial	818:826	arg1	composition					838:848	the microbial community composition	814:848	the microbial community composition in CR mice	814:859	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	4	56	dep	HFD	756:758	arg1	a					744:744	a	744:744	a	744:744	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	0	57	link	gut-derived	56:66	arg1	acid					81:84	gut-derived lipoteichoic acid	56:84	gut-derived lipoteichoic acid	56:84	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	6	58	from	effects	967:973	arg1	contractions					1029:1040	duodenal muscularis externa contractions	1001:1040	duodenal muscularis externa contractions	1001:1040	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	6	58	from	effects	967:973	arg1	neurons					1056:1062	myenteric neurons	1046:1062	myenteric neurons	1046:1062	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	2	59	theme	microbiota	323:332	arg1	role					307:310	the role	303:310	the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility	303:498	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	8	60	theme	CR	1312:1313	arg1	mice					1315:1318	CR mice	1312:1318	CR mice	1312:1318	GF mice had fewer neurons per ganglion compared with CR mice.					
32168415	10	61	theme	myenteric	1531:1539	arg1	neurons					1541:1547	nitrergic myenteric neurons	1521:1547	nitrergic myenteric neurons	1521:1547	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	12	62	from	increase	1755:1762	arg1	LTA					1791:1793	Gram-positive bacterial LTA	1767:1793	Gram-positive bacterial LTA	1767:1793	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	9	63	theme	Gram-positive	1366:1378	arg1	bacteria					1380:1387	Gram-positive bacteria	1366:1387	Gram-positive bacteria	1366:1387	CR mice fed a HFD had increased abundance of Gram-positive bacteria.					
32168415	3	64	theme	gut	563:565	arg1	role					551:554	the role	547:554	the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility	547:668	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	4	65	theme	germ-free	686:694	arg1	mice					732:735	germ-free (GF) and conventionally raised (CR) mice	686:735	germ-free (GF) and conventionally raised (CR) mice	686:735	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	5	66	theme	CR	853:854	arg1	mice					856:859	CR mice	853:859	CR mice	853:859	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	10	67	theme	myenteric	1603:1611	arg1	ganglia					1613:1619	myenteric ganglia	1603:1619	myenteric ganglia	1603:1619	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	1	68	theme	microbial	166:174	arg1	alterations					176:186	microbial alterations	166:186	microbial alterations	166:186	BACKGROUND High-fat diet, microbial alterations and lipopolysaccharide (LPS) are thought to cause enteric diabetic neuropathy and intestinal dysmotility.					
32168415	2	69	theme	intestinal	477:486	arg1	dysmotility					488:498	intestinal dysmotility	477:498	intestinal dysmotility	477:498	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	7	70	dep	RESULTS	1097:1103	arg1	reduced					1129:1135	reduced	1129:1135	reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice	1129:1256	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	8	71	contain	had	1267:1269	arg1	mice					1262:1265	GF mice	1259:1265	GF mice	1259:1265	GF mice had fewer neurons per ganglion compared with CR mice.					
32168415	8	71	contain	had	1267:1269	arg2	neurons					1277:1283	fewer neurons	1271:1283	fewer neurons	1271:1283	GF mice had fewer neurons per ganglion compared with CR mice.					
32168415	0	72	theme	enteric	120:126	arg1	neuropathy					128:137	enteric neuropathy	120:137	enteric neuropathy	120:137	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	4	73	dep	METHODS	671:677	arg1	fed					682:684	fed	682:684	fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks	682:799	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	7	74	theme	GF-HFD	1246:1251	arg1	mice					1253:1256	GF-HFD mice	1246:1256	GF-HFD mice	1246:1256	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	12	75	dep	INFERENCES	1710:1719	arg1	contribute					1799:1808	contribute	1799:1808	may contribute to enteric neuropathy	1795:1830	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	2	76	theme	short-chain	391:401	arg1	SCFAs					416:420	SCFAs	416:420	SCFAs	416:420	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	76	theme	short-chain	391:401	arg1	acids					409:413	short-chain fatty acids	391:413	short-chain fatty acids (SCFAs)	391:421	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	0	77	theme	diet-induced	9:20	arg1	microbiota					41:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota	0:50	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	8	78	theme	fewer	1271:1275	arg1	neurons					1277:1283	fewer neurons	1271:1283	fewer neurons	1271:1283	GF mice had fewer neurons per ganglion compared with CR mice.					
32168415	10	79	theme	neurons	1541:1547	arg1	stress					1573:1578	increased oxidative stress	1553:1578	increased oxidative stress	1553:1578	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	10	79	theme	neurons	1541:1547	arg1	density					1510:1516	the density	1506:1516	the density of nitrergic myenteric neurons	1506:1547	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	10	79	theme	neurons	1541:1547	arg1	production					1589:1598	TNFα production	1584:1598	TNFα production	1584:1598	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	7	80	theme	KEY	1093:1095	arg1	RESULTS					1097:1103	KEY RESULTS	1093:1103	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.	1093:1257	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	5	81	theme	rRNA	871:874	arg1	sequencing					876:885	16S rRNA sequencing	867:885	16S rRNA sequencing	867:885	We analyzed the microbial community composition in CR mice using 16S rRNA sequencing and damage to myenteric neurons using immunohistochemistry.					
32168415	7	82	theme	CR	1232:1233	arg1	duodenum					1220:1227	the duodenum	1216:1227	the duodenum of CR	1216:1233	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	10	83	from	stress	1573:1578	arg1	ganglia					1613:1619	myenteric ganglia	1603:1619	myenteric ganglia	1603:1619	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	3	84	theme	diabetic	615:622	arg1	neuropathy					632:641	diabetic enteric neuropathy	615:641	diabetic enteric neuropathy	615:641	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	4	85	theme	raised	720:725	arg1	mice					732:735	germ-free (GF) and conventionally raised (CR) mice	686:735	germ-free (GF) and conventionally raised (CR) mice	686:735	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	10	86	theme	TNFα	1584:1587	arg1	production					1589:1598	TNFα production	1584:1598	TNFα production	1584:1598	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	6	87	theme	SCFAs	992:996	arg1	effects					967:973	the effects	963:973	the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons	963:1062	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	0	88	theme	gut-derived	56:66	arg1	acid					81:84	gut-derived lipoteichoic acid	56:84	gut-derived lipoteichoic acid	56:84	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	12	89	theme	bacterial	1781:1789	arg1	LTA					1791:1793	Gram-positive bacterial LTA	1767:1793	Gram-positive bacterial LTA	1767:1793	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	0	90	dep	microbiota	41:50	arg1	to					34:35	to	34:35	to	34:35	High-fat diet-induced alterations to gut microbiota and gut-derived lipoteichoic acid contributes to the development of enteric neuropathy.					
32168415	2	91	from	bacteria	378:385	arg1	acid					348:351	lipoteichoic acid	335:351	lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs)	335:421	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	91	from	bacteria	378:385	arg1	LTA					354:356	LTA	354:356	LTA	354:356	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	91	from	bacteria	378:385	arg1	microbiota					323:332	the gut microbiota	315:332	the gut microbiota	315:332	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	92	theme	diabetic	445:452	arg1	neuropathy					462:471	diabetic enteric neuropathy	445:471	diabetic enteric neuropathy	445:471	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	3	93	theme	intestinal	647:656	arg1	dysmotility					658:668	intestinal dysmotility	647:668	intestinal dysmotility	647:668	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	4	94	theme	standard	764:771	arg1	SCD					784:786	SCD	784:786	SCD	784:786	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	4	94	theme	standard	764:771	arg1	diet					778:781	standard chow diet	764:781	standard chow diet (SCD)	764:787	METHODS We fed germ-free (GF) and conventionally raised (CR) mice either a high-fat (HFD) or standard chow diet (SCD) for 8 weeks.					
32168415	12	95	theme	microbial	1725:1733	arg1	alterations					1735:1745	Gut microbial alterations	1721:1745	Gut microbial alterations induced increase in Gram-positive bacterial LTA	1721:1793	CONCLUSIONS & INFERENCES Gut microbial alterations induced increase in Gram-positive bacterial LTA may contribute to enteric neuropathy.					
32168415	3	96	theme	neuropathy	632:641	arg1	development					600:610	the development	596:610	the development of diabetic enteric neuropathy and intestinal dysmotility	596:668	Our aim was to examine the role of the gut microbiota, LTA and SCFAs in the development of diabetic enteric neuropathy and intestinal dysmotility.					
32168415	6	97	theme	muscularis	1010:1019	arg1	externa					1021:1027	duodenal muscularis externa	1001:1027	duodenal muscularis externa contractions	1001:1040	We also studied the effects of LPS, LTA, and SCFAs on duodenal muscularis externa contractions and myenteric neurons using cultured preparations.					
32168415	1	98	theme	enteric	238:244	arg1	neuropathy					255:264	enteric diabetic neuropathy	238:264	enteric diabetic neuropathy	238:264	BACKGROUND High-fat diet, microbial alterations and lipopolysaccharide (LPS) are thought to cause enteric diabetic neuropathy and intestinal dysmotility.					
32168415	2	99	from	acids	409:413	arg1	acid					348:351	lipoteichoic acid	335:351	lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs)	335:421	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	99	from	acids	409:413	arg1	LTA					354:356	LTA	354:356	LTA	354:356	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	2	99	from	acids	409:413	arg1	microbiota					323:332	the gut microbiota	315:332	the gut microbiota	315:332	However, the role of the gut microbiota, lipoteichoic acid (LTA) from Gram-positive bacteria and short-chain fatty acids (SCFAs) in the development of diabetic enteric neuropathy and intestinal dysmotility is not well understood.					
32168415	7	100	theme	myenteric	1182:1190	arg1	neurons					1192:1198	nitrergic myenteric neurons	1172:1198	nitrergic myenteric neurons	1172:1198	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32168415	10	101	theme	increased	1553:1561	arg1	stress					1573:1578	increased oxidative stress	1553:1578	increased oxidative stress	1553:1578	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	10	102	theme	duodenal	1434:1441	arg1	contractions					1454:1465	duodenal muscularis contractions	1434:1465	duodenal muscularis contractions after 24 hours of cultured	1434:1492	LTA and LPS did not affect the frequency of duodenal muscularis contractions after 24 hours of cultured but reduced the density of nitrergic myenteric neurons and increased oxidative stress and TNFα production in myenteric ganglia.					
32168415	7	103	theme	High-fat	1105:1112	arg1	diet					1114:1117	High-fat diet	1105:1117	High-fat diet ingestion	1105:1127	KEY RESULTS High-fat diet ingestion reduced the total number and the number of nitrergic myenteric neurons per ganglion in the duodenum of CR but not in GF-HFD mice.					
32713693	13	0	theme	age	2439:2441	arg1	d					2428:2428	d 57-70	2428:2434	d 57-70 of age	2428:2441	The postweaning (d 57-70 of age) ADG was greater for calves fed the 4-min diet compared with those fed the CON diet.					
32713693	3	1	theme	housed	825:830	arg1	calves					832:837	individually housed calves	812:837	individually housed calves	812:837	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	11	2	theme	volatile	2157:2164	arg1	profile					2177:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	9	3	theme	crude	1774:1778	arg1	protein					1780:1786	crude protein	1774:1786	crude protein	1774:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	0	4	theme	female	167:172	arg1	calves					180:185	Holstein female dairy calves	158:185	Holstein female dairy calves	158:185	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	6	5	theme	retention	1150:1158	arg1	time					1160:1163	conditioner retention time	1138:1163	conditioner retention time	1138:1163	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	15	6	theme	postweaning	2859:2869	arg1	ADG					2871:2873	postweaning ADG	2859:2873	postweaning ADG	2859:2873	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	0	7	theme	calves	180:185	arg1	metabolites					126:136	blood metabolites	120:136	blood metabolites	120:136	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	0	7	theme	calves	180:185	arg1	performance					143:153	performance	143:153	performance of Holstein female dairy calves	143:185	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	0	7	theme	calves	180:185	arg1	digestibility					83:95	nutrient digestibility	74:95	nutrient digestibility	74:95	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	0	7	theme	calves	180:185	arg1	fermentation					106:117	ruminal fermentation	98:117	ruminal fermentation	98:117	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	1	8	theme	pelleting	262:270	arg1	process					272:278	the pelleting process	258:278	the pelleting process of starter feed	258:294	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	11	9	theme	retention	2243:2251	arg1	times					2253:2257	different conditioner retention times	2221:2257	different conditioner retention times	2221:2257	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	8	10	theme	matter	1554:1559	arg1	intake					1561:1566	feed dry matter intake	1545:1566	feed dry matter intake	1545:1566	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	15	11	theme	calves	2901:2906	arg1	similar					2912:2918	similar	2912:2918	similar	2912:2918	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	15	11	theme	calves	2901:2906	arg1	BW					2895:2896	the final BW	2885:2896	the final BW of calves	2885:2906	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	9	12	theme	retention	1976:1984	arg1	time					1986:1989	conditioner retention time	1964:1989	conditioner retention time	1964:1989	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	4	13	dep	d	950:950	arg1	to					954:955	to	954:955	to	954:955	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	10	14	theme	average	2060:2066	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	14	theme	average	2060:2066	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	5	15	theme	age	1112:1114	arg1	d					1104:1104	d 70	1104:1107	d 70 of age	1104:1114	All calves were weaned on d 56 of age and remained in the study until d 70 of age.					
32713693	14	16	theme	starter	2597:2603	arg1	feed					2605:2608	starter feed	2597:2608	starter feed	2597:2608	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	7	17	theme	retention	1423:1431	arg1	time					1433:1436	conditioner retention time	1411:1436	conditioner retention time	1411:1436	Additionally, the pellet durability and hardness also linearly increased with increasing conditioner retention time during pelleting.					
32713693	4	18	theme	milk	909:912	arg1	L/d					884:886	4 L/d	882:886	4 L/d of pasteurized whole milk	882:912	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	8	19	theme	energy	1583:1588	arg1	intake					1590:1595	metabolizable energy intake	1569:1595	metabolizable energy intake	1569:1595	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	12	20	theme	ammonia	2272:2278	arg1	concentration					2280:2292	The ruminal ammonia concentration	2260:2292	The ruminal ammonia concentration	2260:2292	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	12	20	theme	ammonia	2272:2278	arg1	lower					2307:2311	lower	2307:2311	lower	2307:2311	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	14	21	theme	starch	2642:2647	arg1	gelatinization					2624:2637	the gelatinization	2620:2637	the gelatinization of starch, durability, and hardness of the pellets	2620:2688	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	10	22	located	found	2043:2047	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	22	located	found	2043:2047	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	22	located	found	2043:2047	arg2	difference					2028:2037	No difference	2025:2037	No difference	2025:2037	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	22	located	found	2043:2047	arg1	rates					2095:2099	growth rates	2088:2099	growth rates of hip height, withers height, and heart girth	2088:2146	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	9	23	theme	age	1818:1820	arg1	d					1804:1804	d 49 to 56	1804:1813	d 49 to 56 of age	1804:1820	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	14	24	theme	skeletal	2746:2753	arg1	growth					2755:2760	skeletal growth	2746:2760	skeletal growth	2746:2760	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	9	25	theme	feed	2019:2022	arg1	pelleting					1998:2006	pelleting	1998:2006	pelleting of starter feed	1998:2022	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	9	26	dep	d	1804:1804	arg1	to					1809:1810	to	1809:1810	to	1809:1810	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	0	27	theme	Holstein	158:165	arg1	calves					180:185	Holstein female dairy calves	158:185	Holstein female dairy calves	158:185	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	10	28	theme	hip	2104:2106	arg1	height					2108:2113	hip height	2104:2113	hip height	2104:2113	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	1	29	theme	dairy	398:402	arg1	calves					404:409	dairy calves	398:409	dairy calves	398:409	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	6	30	theme	starter	1254:1260	arg1	content					1234:1240	the gelatinized starch content	1211:1240	the gelatinized starch content of pelleted starter	1211:1260	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	3	31	theme	similar	710:716	arg1	composition					730:740	similar nutritional composition	710:740	similar nutritional composition	710:740	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	9	32	dep	weaning	1795:1801	arg1	d					1804:1804	d 49 to 56	1804:1813	d 49 to 56 of age	1804:1820	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	4	33	theme	calf	963:966	arg1	age					968:970	calf age	963:970	calf age	963:970	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	11	34	theme	different	2221:2229	arg1	times					2253:2257	different conditioner retention times	2221:2257	different conditioner retention times	2221:2257	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	8	35	theme	final	1610:1614	arg1	BW					1616:1617	final BW	1610:1617	final BW	1610:1617	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	6	36	theme	gelatinized	1215:1225	arg1	content					1234:1240	the gelatinized starch content	1211:1240	the gelatinized starch content of pelleted starter	1211:1260	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	13	37	theme	CON	2518:2520	arg1	diet					2522:2525	the CON diet	2514:2525	the CON diet	2514:2525	The postweaning (d 57-70 of age) ADG was greater for calves fed the 4-min diet compared with those fed the CON diet.					
32713693	10	38	theme	height	2124:2129	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	38	theme	height	2124:2129	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	38	theme	height	2124:2129	arg1	rates					2095:2099	growth rates	2088:2099	growth rates of hip height, withers height, and heart girth	2088:2146	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	0	39	theme	starter	58:64	arg1	pelleting					45:53	pelleting	45:53	pelleting of starter	45:64	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	8	40	theme	pelleted	1464:1471	arg1	feed					1473:1476	Feeding pelleted feed	1456:1476	Feeding pelleted feed prepared using different conditioner retention time	1456:1528	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	3	41	theme	pelleted	683:690	arg1	starter					692:698	Three pelleted starter	677:698	Three pelleted starter	677:698	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	8	42	theme	feed	1623:1626	arg1	efficiency					1628:1637	feed efficiency	1623:1637	feed efficiency	1623:1637	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	1	43	theme	ruminal	331:337	arg1	fermentation					339:350	ruminal fermentation	331:350	ruminal fermentation	331:350	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	3	44	dep	feeds	700:704	arg1	had					706:708	had	706:708	feeds had similar nutritional composition	700:740	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	8	45	theme	feed	1545:1548	arg1	intake					1561:1566	feed dry matter intake	1545:1566	feed dry matter intake	1545:1566	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	14	46	theme	feed	2725:2728	arg1	efficiency					2730:2739	feed efficiency	2725:2739	feed efficiency	2725:2739	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	10	47	theme	girth	2142:2146	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	47	theme	girth	2142:2146	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	47	theme	girth	2142:2146	arg1	rates					2095:2099	growth rates	2088:2099	growth rates of hip height, withers height, and heart girth	2088:2146	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	2	48	dep	calves	448:453	arg1	assigned					504:511	assigned	504:511	were randomly assigned to 1 of 3 treatments	490:532	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	8	49	theme	different	1493:1501	arg1	time					1525:1528	different conditioner retention time	1493:1528	different conditioner retention time	1493:1528	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	2	50	dep	pelleted	589:596	arg1	conditioned					606:616	conditioned	606:616	conditioned for 2 min	606:626	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	2	50	dep	pelleted	589:596	arg1	2					586:586	2	586:586	2	586:586	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	11	51	theme	conditioner	2231:2241	arg1	times					2253:2257	different conditioner retention times	2221:2257	different conditioner retention times	2221:2257	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	2	52	theme	weight	477:482	arg1	kg					466:467	[40 ± 1.93 kg	455:467	[40 ± 1.93 kg of body weight (BW)]	455:488	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	4	53	theme	morning	994:1000	arg1	feeding					1002:1008	morning feeding	994:1008	morning feeding	994:1008	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	9	54	theme	cage	1861:1864	arg1	d					1842:1842	d 63 to 70	1842:1851	d 63 to 70 of calf cage	1842:1864	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	0	55	theme	ruminal	98:104	arg1	fermentation					106:117	ruminal fermentation	98:117	ruminal fermentation	98:117	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	8	56	theme	retention	1515:1523	arg1	time					1525:1528	different conditioner retention time	1493:1528	different conditioner retention time	1493:1528	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	2	57	theme	Holstein	426:433	arg1	calves					448:453	30 Holstein female dairy calves	423:453	30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments	423:532	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	14	58	theme	pellets	2682:2688	arg1	durability					2650:2659	durability	2650:2659	durability	2650:2659	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	14	58	theme	pellets	2682:2688	arg1	hardness					2666:2673	hardness	2666:2673	hardness	2666:2673	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	14	58	theme	pellets	2682:2688	arg1	starch					2642:2647	starch	2642:2647	starch	2642:2647	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	3	59	theme	chopped	781:787	arg1	straw					795:799	3% chopped wheat straw	778:799	3% chopped wheat straw	778:799	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	9	60	theme	apparent	1703:1710	arg1	digestibility					1712:1724	the total-tract apparent digestibility	1687:1724	the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein	1687:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	0	61	theme	conditioner	11:21	arg1	time					33:36	conditioner retention time	11:36	conditioner retention time	11:36	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	1	62	theme	starter	283:289	arg1	feed					291:294	starter feed	283:294	starter feed	283:294	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	9	63	dep	d	1842:1842	arg1	to					1847:1848	to	1847:1848	to	1847:1848	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	3	64	theme	3	778:778	arg1	%					779:779	%	779:779	%	779:779	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	12	65	theme	4-min	2332:2336	arg1	diet					2338:2341	the 4-min diet	2328:2341	the 4-min diet	2328:2341	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	15	66	theme	final	2889:2893	arg1	similar					2912:2918	similar	2912:2918	similar	2912:2918	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	15	66	theme	final	2889:2893	arg1	BW					2895:2896	the final BW	2885:2896	the final BW of calves	2885:2906	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	0	67	theme	time	33:36	arg1	Effects					0:6	Effects	0:6	Effects of conditioner retention time during pelleting of starter	0:64	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	9	68	dep	weaning	1833:1839	arg1	d					1842:1842	d 63 to 70	1842:1851	d 63 to 70 of calf cage	1842:1864	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	2	69	theme	dairy	442:446	arg1	calves					448:453	30 Holstein female dairy calves	423:453	30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments	423:532	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	4	70	theme	age	1029:1031	arg1	d					1024:1024	51 to 56 d	1015:1024	51 to 56 d of age	1015:1031	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	9	71	theme	dry	1898:1900	arg1	matter					1902:1907	dry matter	1898:1907	dry matter	1898:1907	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	13	72	theme	4-min	2479:2483	arg1	diet					2485:2488	the 4-min diet	2475:2488	the 4-min diet	2475:2488	The postweaning (d 57-70 of age) ADG was greater for calves fed the 4-min diet compared with those fed the CON diet.					
32713693	9	73	theme	neutral	1745:1751	arg1	fiber					1763:1767	neutral detergent fiber	1745:1767	neutral detergent fiber	1745:1767	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	6	74	theme	starch	1227:1232	arg1	content					1234:1240	the gelatinized starch content	1211:1240	the gelatinized starch content of pelleted starter	1211:1260	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	4	75	theme	whole	903:907	arg1	milk					909:912	pasteurized whole milk	891:912	pasteurized whole milk	891:912	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	9	76	theme	fiber	1763:1767	arg1	digestibility					1712:1724	the total-tract apparent digestibility	1687:1724	the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein	1687:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	6	77	theme	pellet	1172:1177	arg1	processing					1179:1188	pellet processing	1172:1188	pellet processing for 0, 2, and 4 min	1172:1208	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	1	78	theme	conditioner	224:234	arg1	time					246:249	conditioner retention time	224:249	conditioner retention time during the pelleting process of starter feed	224:294	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	9	79	from	differences	1672:1682	arg1	digestibility					1712:1724	the total-tract apparent digestibility	1687:1724	the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein	1687:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	0	80	theme	dairy	174:178	arg1	calves					180:185	Holstein female dairy calves	158:185	Holstein female dairy calves	158:185	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	6	81	theme	time	1160:1163	arg1	increase					1126:1133	the increase	1122:1133	the increase of conditioner retention time during pellet processing for 0, 2, and 4 min	1122:1208	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	1	82	theme	time	246:249	arg1	effects					213:219	the effects	209:219	the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves	209:409	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	9	83	theme	matter	1737:1742	arg1	digestibility					1712:1724	the total-tract apparent digestibility	1687:1724	the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein	1687:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	11	84	theme	fatty	2166:2170	arg1	profile					2177:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	9	85	theme	protein	1780:1786	arg1	digestibility					1712:1724	the total-tract apparent digestibility	1687:1724	the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein	1687:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	8	86	theme	dry	1550:1552	arg1	intake					1561:1566	feed dry matter intake	1545:1566	feed dry matter intake	1545:1566	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	6	87	theme	conditioner	1138:1148	arg1	time					1160:1163	conditioner retention time	1138:1163	conditioner retention time	1138:1163	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	13	88	theme	postweaning	2415:2425	arg1	greater					2452:2458	greater	2452:2458	greater	2452:2458	The postweaning (d 57-70 of age) ADG was greater for calves fed the 4-min diet compared with those fed the CON diet.					
32713693	13	88	theme	postweaning	2415:2425	arg1	ADG					2444:2446	The postweaning (d 57-70 of age) ADG	2411:2446	The postweaning (d 57-70 of age) ADG	2411:2446	The postweaning (d 57-70 of age) ADG was greater for calves fed the 4-min diet compared with those fed the CON diet.					
32713693	15	89	theme	pelleting	2832:2840	arg1	process					2842:2848	the pelleting process	2828:2848	the pelleting process	2828:2848	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	11	90	theme	Ruminal	2149:2155	arg1	profile					2177:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	9	91	theme	conditioner	1964:1974	arg1	time					1986:1989	conditioner retention time	1964:1989	conditioner retention time	1964:1989	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	10	92	theme	daily	2068:2072	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	92	theme	daily	2068:2072	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	14	93	theme	conditioning	2547:2558	arg1	time					2560:2563	the conditioning time	2543:2563	the conditioning time during the pelleting process of starter feed	2543:2608	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	8	94	theme	metabolizable	1569:1581	arg1	intake					1590:1595	metabolizable energy intake	1569:1595	metabolizable energy intake	1569:1595	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	14	95	theme	feed	2605:2608	arg1	process					2586:2592	the pelleting process	2572:2592	the pelleting process of starter feed	2572:2608	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	15	96	theme	conditioning	2803:2814	arg1	time					2816:2819	conditioning time	2803:2819	conditioning time	2803:2819	Increasing conditioning time during the pelleting process improved postweaning ADG; however, the final BW of calves was similar among treatments.					
32713693	7	97	theme	conditioner	1411:1421	arg1	time					1433:1436	conditioner retention time	1411:1436	conditioner retention time	1411:1436	Additionally, the pellet durability and hardness also linearly increased with increasing conditioner retention time during pelleting.					
32713693	10	98	theme	overall	2052:2058	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	98	theme	overall	2052:2058	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	13	99	dep	ADG	2444:2446	arg1	d					2428:2428	d 57-70	2428:2434	d 57-70 of age	2428:2441	The postweaning (d 57-70 of age) ADG was greater for calves fed the 4-min diet compared with those fed the CON diet.					
32713693	0	100	theme	blood	120:124	arg1	metabolites					126:136	blood metabolites	120:136	blood metabolites	120:136	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	2	101	dep	pelleted	539:546	arg1	total					414:418	A total	412:418	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments	412:532	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	2	101	dep	pelleted	539:546	arg1	1					536:536	1	536:536	1	536:536	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	8	102	theme	weaning	1598:1604	arg1	BW					1606:1607	weaning BW	1598:1607	weaning BW	1598:1607	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	1	103	theme	growth	376:381	arg1	performance					383:393	growth performance	376:393	growth performance	376:393	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	9	104	theme	starter	2011:2017	arg1	feed					2019:2022	starter feed	2011:2022	starter feed	2011:2022	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	14	105	theme	pelleting	2576:2584	arg1	process					2586:2592	the pelleting process	2572:2592	the pelleting process of starter feed	2572:2608	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	12	106	theme	ruminal	2264:2270	arg1	concentration					2280:2292	The ruminal ammonia concentration	2260:2292	The ruminal ammonia concentration	2260:2292	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	12	106	theme	ruminal	2264:2270	arg1	lower					2307:2311	lower	2307:2311	lower	2307:2311	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	4	107	theme	age	968:970	arg1	d					950:950	d 3 to 50	950:958	d 3 to 50 of calf age	950:970	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	3	108	theme	age	857:859	arg1	d					844:844	d 3 to 70	844:852	d 3 to 70 of age	844:859	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	1	109	from	effects	213:219	arg1	performance					383:393	growth performance	376:393	growth performance	376:393	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	109	from	effects	213:219	arg1	intake					299:304	intake	299:304	intake	299:304	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	109	from	effects	213:219	arg1	digestibility					316:328	nutrient digestibility	307:328	nutrient digestibility	307:328	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	109	from	effects	213:219	arg1	fermentation					339:350	ruminal fermentation	331:350	ruminal fermentation	331:350	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	109	from	effects	213:219	arg1	metabolites					359:369	blood metabolites	353:369	blood metabolites	353:369	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	110	theme	calves	404:409	arg1	performance					383:393	growth performance	376:393	growth performance	376:393	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	110	theme	calves	404:409	arg1	intake					299:304	intake	299:304	intake	299:304	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	110	theme	calves	404:409	arg1	digestibility					316:328	nutrient digestibility	307:328	nutrient digestibility	307:328	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	110	theme	calves	404:409	arg1	fermentation					339:350	ruminal fermentation	331:350	ruminal fermentation	331:350	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	1	110	theme	calves	404:409	arg1	metabolites					359:369	blood metabolites	353:369	blood metabolites	353:369	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	6	111	theme	pelleted	1245:1252	arg1	starter					1254:1260	pelleted starter	1245:1260	pelleted starter	1245:1260	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	3	112	theme	nutritional	718:728	arg1	composition					730:740	similar nutritional composition	710:740	similar nutritional composition	710:740	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	4	113	dep	56	1021:1022	arg1	to					1018:1019	to	1018:1019	to	1018:1019	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	10	114	theme	growth	2088:2093	arg1	rates					2095:2099	growth rates	2088:2099	growth rates of hip height, withers height, and heart girth	2088:2146	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	14	115	theme	hardness	2666:2673	arg1	gelatinization					2624:2637	the gelatinization	2620:2637	the gelatinization of starch, durability, and hardness of the pellets	2620:2688	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	12	116	theme	postweaning	2391:2401	arg1	period					2403:2408	the postweaning period	2387:2408	the postweaning period	2387:2408	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	10	117	theme	withers	2116:2122	arg1	height					2124:2129	withers height	2116:2129	withers height	2116:2129	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	0	118	theme	nutrient	74:81	arg1	digestibility					83:95	nutrient digestibility	74:95	nutrient digestibility	74:95	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	8	119	theme	Feeding	1456:1462	arg1	feed					1473:1476	Feeding pelleted feed	1456:1476	Feeding pelleted feed prepared using different conditioner retention time	1456:1528	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	2	120	theme	±	459:459	arg1	kg					466:467	[40 ± 1.93 kg	455:467	[40 ± 1.93 kg of body weight (BW)]	455:488	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	14	121	theme	age	2787:2789	arg1	d					2782:2782	the first 70 d	2769:2782	the first 70 d of age	2769:2789	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	10	122	theme	height	2108:2113	arg1	ADG					2080:2082	ADG	2080:2082	ADG	2080:2082	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	122	theme	height	2108:2113	arg1	gain					2074:2077	overall average daily gain	2052:2077	overall average daily gain (ADG)	2052:2083	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	10	122	theme	height	2108:2113	arg1	rates					2095:2099	growth rates	2088:2099	growth rates of hip height, withers height, and heart girth	2088:2146	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	14	123	theme	feed	2712:2715	arg1	intake					2717:2722	feed intake	2712:2722	feed intake	2712:2722	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	6	124	dep	feed	1262:1265	arg1	increased					1276:1284	increased	1276:1284	increased	1276:1284	With the increase of conditioner retention time during pellet processing for 0, 2, and 4 min, the gelatinized starch content of pelleted starter feed linearly increased from 14, 30, and 45%, respectively.					
32713693	12	125	theme	CON	2371:2373	arg1	diet					2375:2378	the CON diet	2367:2378	the CON diet during the postweaning period	2367:2408	The ruminal ammonia concentration tended to be lower for calves fed the 4-min diet compared with those fed the CON diet during the postweaning period.					
32713693	4	126	theme	feeding	1002:1008	arg1	L/d					987:989	2 L/d	985:989	2 L/d of morning feeding from 51 to 56 d of age	985:1031	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	10	127	theme	heart	2136:2140	arg1	girth					2142:2146	heart girth	2136:2146	heart girth	2136:2146	No difference was found in overall average daily gain (ADG) or growth rates of hip height, withers height, and heart girth.					
32713693	9	128	theme	calf	1856:1859	arg1	cage					1861:1864	calf cage	1856:1864	calf cage	1856:1864	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	1	129	theme	blood	353:357	arg1	metabolites					359:369	blood metabolites	353:369	blood metabolites	353:369	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	7	130	theme	pellet	1340:1345	arg1	durability					1347:1356	the pellet durability	1336:1356	the pellet durability	1336:1356	Additionally, the pellet durability and hardness also linearly increased with increasing conditioner retention time during pelleting.					
32713693	2	131	theme	body	472:475	arg1	weight					477:482	body weight	472:482	body weight (BW)]	472:488	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	2	131	theme	body	472:475	arg1	BW					485:486	BW	485:486	BW	485:486	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	14	132	theme	first	2773:2777	arg1	d					2782:2782	the first 70 d	2769:2782	the first 70 d of age	2769:2789	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	8	133	theme	conditioner	1503:1513	arg1	time					1525:1528	different conditioner retention time	1493:1528	different conditioner retention time	1493:1528	Feeding pelleted feed prepared using different conditioner retention time did not affect feed dry matter intake, metabolizable energy intake, weaning BW, final BW, or feed efficiency during the study.					
32713693	9	134	theme	total-tract	1691:1701	arg1	digestibility					1712:1724	the total-tract apparent digestibility	1687:1724	the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein	1687:1786	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	0	135	theme	retention	23:31	arg1	time					33:36	conditioner retention time	11:36	conditioner retention time	11:36	Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves.					
32713693	3	136	theme	wheat	789:793	arg1	straw					795:799	3% chopped wheat straw	778:799	3% chopped wheat straw	778:799	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	11	137	theme	acid	2172:2175	arg1	profile					2177:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile	2149:2183	Ruminal volatile fatty acid profile was not affected by pelleting under different conditioner retention times.					
32713693	1	138	theme	feed	291:294	arg1	process					272:278	the pelleting process	258:278	the pelleting process of starter feed	258:294	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	3	139	theme	%	779:779	arg1	straw					795:799	3% chopped wheat straw	778:799	3% chopped wheat straw	778:799	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	2	140	theme	calves	448:453	arg1	total					414:418	A total	412:418	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments	412:532	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	4	141	from	d	1024:1024	arg1	L/d					987:989	2 L/d	985:989	2 L/d of morning feeding from 51 to 56 d of age	985:1031	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	9	142	theme	organic	1729:1735	arg1	matter					1737:1742	organic matter	1729:1742	organic matter	1729:1742	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	2	143	theme	female	435:440	arg1	calves					448:453	30 Holstein female dairy calves	423:453	30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments	423:532	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	9	144	theme	matter	1902:1907	arg1	digestibility					1881:1893	the digestibility	1877:1893	the digestibility of dry matter and starch after weaning	1877:1932	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	3	145	dep	d	844:844	arg1	to					848:849	to	848:849	to	848:849	Three pelleted starter feeds had similar nutritional composition, and the starters were blended with 3% chopped wheat straw and fed to individually housed calves from d 3 to 70 of age.					
32713693	1	146	theme	nutrient	307:314	arg1	digestibility					316:328	nutrient digestibility	307:328	nutrient digestibility	307:328	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
32713693	2	147	dep	pelleted	637:644	arg1	3					634:634	3	634:634	3	634:634	A total of 30 Holstein female dairy calves [40 ± 1.93 kg of body weight (BW)] were randomly assigned to 1 of 3 treatments: (1) pelleted starter conditioned for 0 min (CON), (2) pelleted starter conditioned for 2 min, and (3) pelleted starter conditioned for 4 min.					
32713693	9	148	theme	detergent	1753:1761	arg1	fiber					1763:1767	neutral detergent fiber	1745:1767	neutral detergent fiber	1745:1767	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	5	149	theme	age	1068:1070	arg1	d					1060:1060	d 56	1060:1063	d 56 of age	1060:1070	All calves were weaned on d 56 of age and remained in the study until d 70 of age.					
32713693	9	150	theme	starch	1913:1918	arg1	digestibility					1881:1893	the digestibility	1877:1893	the digestibility of dry matter and starch after weaning	1877:1932	We observed no differences in the total-tract apparent digestibility of organic matter, neutral detergent fiber, and crude protein around weaning (d 49 to 56 of age) and after weaning (d 63 to 70 of calf cage); however, the digestibility of dry matter and starch after weaning was increased with increasing conditioner retention time during pelleting of starter feed.					
32713693	4	151	from	d	950:950	arg1	h					943:943	0800 and 1600 h	929:943	0800 and 1600 h from d 3 to 50 of calf age	929:970	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	4	152	theme	pasteurized	891:901	arg1	milk					909:912	pasteurized whole milk	891:912	pasteurized whole milk	891:912	All calves were fed 4 L/d of pasteurized whole milk twice daily at 0800 and 1600 h from d 3 to 50 of calf age, followed by 2 L/d of morning feeding from 51 to 56 d of age.					
32713693	14	153	theme	durability	2650:2659	arg1	gelatinization					2624:2637	the gelatinization	2620:2637	the gelatinization of starch, durability, and hardness of the pellets	2620:2688	In conclusion, the conditioning time during the pelleting process of starter feed increased the gelatinization of starch, durability, and hardness of the pellets but did not influence feed intake, feed efficiency, and skeletal growth during the first 70 d of age.					
32713693	1	154	theme	retention	236:244	arg1	time					246:249	conditioner retention time	224:249	conditioner retention time during the pelleting process of starter feed	224:294	This study evaluated the effects of conditioner retention time during the pelleting process of starter feed on intake, nutrient digestibility, ruminal fermentation, blood metabolites, and growth performance of dairy calves.					
33671105	2	0	theme	carbon	508:513	arg1	saturation					515:524	carbon saturation	508:524	carbon saturation	508:524	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	5	1	theme	DPPG	1257:1260	arg1	monolayer					1262:1270	the DPPG monolayer	1253:1270	the DPPG monolayer driven very likely by the electrostatic attraction	1253:1321	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	4	2	theme	Langmuir	766:773	arg1	stability					780:788	a Langmuir film stability	764:788	a Langmuir film stability	764:788	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	6	3	from	differences	1328:1338	arg1	strength					1358:1365	the adsorption strength	1343:1365	the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines	1343:1457	The differences in the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines were also observed.					
33671105	2	4	theme	membrane	364:371	arg1	models					373:378	The cell membrane models	355:378	The cell membrane models	355:378	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	0	5	from	Effect	0:5	arg1	Nanomedicine					87:98	Nanomedicine	87:98	Nanomedicine	87:98	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	0	5	from	Effect	0:5	arg1	Monolayers					133:142	DPPG, DSPC, and POPC Langmuir Monolayers	103:142	DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models	103:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	6	6	theme	Fe3O4-AChit	1374:1384	arg1	nanoparticles					1386:1398	the Fe3O4-AChit nanoparticles	1370:1398	the Fe3O4-AChit nanoparticles	1370:1398	The differences in the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines were also observed.					
33671105	0	7	theme	DPPG	103:106	arg1	Monolayers					133:142	DPPG, DSPC, and POPC Langmuir Monolayers	103:142	DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models	103:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	1	8	theme	application	278:288	arg1	potential					290:298	application potential	278:298	application potential	278:298	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	2	9	theme	chains	535:540	arg1	saturation					515:524	carbon saturation	508:524	carbon saturation	508:524	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	2	9	theme	chains	535:540	arg1	head-groups					474:484	different polar head-groups	458:484	different polar head-groups as well as length and carbon saturation of alkyl chains	458:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	2	9	theme	chains	535:540	arg1	length					497:502	length	497:502	length	497:502	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	5	10	theme	investigated	1221:1232	arg1	nanoparticles					1234:1246	the investigated nanoparticles	1217:1246	the investigated nanoparticles	1217:1246	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	6	11	theme	Langmuir	1409:1416	arg1	films					1418:1422	the Langmuir films	1405:1422	the Langmuir films formed by the phosphatidylcholines	1405:1457	The differences in the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines were also observed.					
33671105	2	12	theme	selected	430:437	arg1	phospholipids					439:451	three selected phospholipids	424:451	three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains	424:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	4	13	theme	Fe3O4-AChit	735:745	arg1	nanoparticles					747:759	the positively charged Fe3O4-AChit nanoparticles	712:759	the positively charged Fe3O4-AChit nanoparticles	712:759	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	0	14	theme	DSPC	109:112	arg1	Monolayers					133:142	DPPG, DSPC, and POPC Langmuir Monolayers	103:142	DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models	103:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	0	15	from	Nanoparticles	41:53	arg1	Nanomedicine					87:98	Nanomedicine	87:98	Nanomedicine	87:98	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	5	16	theme	adsorption	1094:1103	arg1	kinetics					1105:1112	The adsorption kinetics	1090:1112	The adsorption kinetics recorded during penetration experiments	1090:1152	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	2	17	theme	Langmuir	400:407	arg1	technique					409:417	a Langmuir technique	398:417	a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains	398:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	1	18	theme	adsorption	172:181	arg1	process					183:189	An adsorption process	169:189	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models	169:340	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	2	19	theme	polar	468:472	arg1	head-groups					474:484	different polar head-groups	458:484	different polar head-groups as well as length and carbon saturation of alkyl chains	458:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	5	20	theme	electrostatic	1298:1310	arg1	attraction					1312:1321	the electrostatic attraction	1294:1321	the electrostatic attraction	1294:1321	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	6	21	theme	nanoparticles	1386:1398	arg1	strength					1358:1365	the adsorption strength	1343:1365	the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines	1343:1457	The differences in the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines were also observed.					
33671105	4	22	theme	charged	727:733	arg1	nanoparticles					747:759	the positively charged Fe3O4-AChit nanoparticles	712:759	the positively charged Fe3O4-AChit nanoparticles	712:759	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	0	23	theme	Chitosan-Coated	19:33	arg1	Nanoparticles					41:53	Aminated Chitosan-Coated Fe3O4 Nanoparticles	10:53	Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine	10:98	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	4	24	theme	these	848:852	arg1	case					840:843	the case	836:843	the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG)	836:942	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	0	25	theme	POPC	119:122	arg1	Monolayers					133:142	DPPG, DSPC, and POPC Langmuir Monolayers	103:142	DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models	103:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	7	26	theme	packed	1536:1541	arg1	monolayer					1548:1556	more loosely packed POPC monolayer	1523:1556	more loosely packed POPC monolayer	1523:1556	The nanoparticles adsorbed more easily into more loosely packed POPC monolayer.					
33671105	4	27	from	influence	699:707	arg1	stability					780:788	a Langmuir film stability	764:788	a Langmuir film stability	764:788	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	27	from	influence	699:707	arg1	state					797:801	phase state	791:801	phase state	791:801	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	27	from	influence	699:707	arg1	textures					808:815	textures	808:815	textures	808:815	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	0	28	theme	Aminated	10:17	arg1	Nanoparticles					41:53	Aminated Chitosan-Coated Fe3O4 Nanoparticles	10:53	Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine	10:98	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	5	29	theme	nanoparticles	1234:1246	arg1	adsorption					1203:1212	the strongest adsorption	1189:1212	the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction	1189:1321	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	1	30	theme	magnetite	194:202	arg1	nanoparticles					204:216	magnetite nanoparticles	194:216	magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models	194:340	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	0	31	theme	Nanoparticles	41:53	arg1	Effect					0:5	Effect	0:5	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.	0:167	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	0	32	from	Nanomedicine	87:98	arg1	Effect					0:5	Effect	0:5	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.	0:167	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	3	33	theme	model	613:617	arg1	regulation					641:650	membrane model composition-dependent regulation	604:650	membrane model composition-dependent regulation of phospholipid-nanoparticle interactions	604:692	The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions.					
33671105	1	34	theme	nanoparticles	204:216	arg1	process					183:189	An adsorption process	169:189	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models	169:340	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	0	35	theme	Fe3O4	35:39	arg1	Nanoparticles					41:53	Aminated Chitosan-Coated Fe3O4 Nanoparticles	10:53	Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine	10:98	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	1	36	theme	cell	321:324	arg1	models					335:340	cell membrane models	321:340	cell membrane models	321:340	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	0	37	theme	Langmuir	124:131	arg1	Monolayers					133:142	DPPG, DSPC, and POPC Langmuir Monolayers	103:142	DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models	103:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	2	38	theme	different	458:466	arg1	head-groups					474:484	different polar head-groups	458:484	different polar head-groups as well as length and carbon saturation of alkyl chains	458:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	2	39	theme	alkyl	529:533	arg1	chains					535:540	alkyl chains	529:540	alkyl chains	529:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	0	40	theme	Applicational	60:72	arg1	Potential					74:82	Applicational Potential	60:82	Applicational Potential	60:82	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	1	41	theme	membrane	326:333	arg1	models					335:340	cell membrane models	321:340	cell membrane models	321:340	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	0	42	theme	Cell	147:150	arg1	Models					161:166	Cell Membrane Models	147:166	Cell Membrane Models	147:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	4	43	theme	charged	875:881	arg1	1-glycerol					925:934	1-glycerol	925:934	1-glycerol	925:934	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	43	theme	charged	875:881	arg1	DPPG					938:941	DPPG	938:941	DPPG	938:941	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	43	theme	charged	875:881	arg1	1,2-dipalmitoyl-sn-glycero-3-phospho-rac-					883:923	negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-	864:923	negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG)	864:942	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	2	44	with	phospholipids	439:451	arg1	head-groups					474:484	different polar head-groups	458:484	different polar head-groups as well as length and carbon saturation of alkyl chains	458:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	2	44	with	phospholipids	439:451	arg1	length					497:502	length	497:502	length	497:502	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	2	45	theme	cell	359:362	arg1	models					373:378	The cell membrane models	355:378	The cell membrane models	355:378	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	3	46	theme	membrane	604:611	arg1	regulation					641:650	membrane model composition-dependent regulation	604:650	membrane model composition-dependent regulation of phospholipid-nanoparticle interactions	604:692	The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions.					
33671105	4	47	theme	film	775:778	arg1	stability					780:788	a Langmuir film stability	764:788	a Langmuir film stability	764:788	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	48	theme	zwitterionic	966:977	arg1	DSPC					1023:1026	DSPC	1023:1026	DSPC	1023:1026	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	48	theme	zwitterionic	966:977	arg1	1,2-distearoyl-sn-glycero-3-phosphocholine					979:1020	zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine	966:1020	zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)	966:1027	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	6	49	theme	adsorption	1347:1356	arg1	strength					1358:1365	the adsorption strength	1343:1365	the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines	1343:1457	The differences in the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines were also observed.					
33671105	3	50	theme	phospholipid-nanoparticle	655:679	arg1	interactions					681:692	phospholipid-nanoparticle interactions	655:692	phospholipid-nanoparticle interactions	655:692	The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions.					
33671105	3	51	theme	composition-dependent	619:639	arg1	regulation					641:650	membrane model composition-dependent regulation	604:650	membrane model composition-dependent regulation of phospholipid-nanoparticle interactions	604:692	The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions.					
33671105	4	52	theme	phase	791:795	arg1	state					797:801	phase state	791:801	phase state	791:801	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	2	53	from	phospholipids	439:451	arg1	technique					409:417	a Langmuir technique	398:417	a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains	398:540	The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains.					
33671105	0	54	theme	Membrane	152:159	arg1	Models					161:166	Cell Membrane Models	147:166	Cell Membrane Models	147:166	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	3	55	theme	interactions	681:692	arg1	regulation					641:650	membrane model composition-dependent regulation	604:650	membrane model composition-dependent regulation of phospholipid-nanoparticle interactions	604:692	The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions.					
33671105	5	56	theme	strongest	1193:1201	arg1	adsorption					1203:1212	the strongest adsorption	1189:1212	the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction	1189:1321	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	7	57	theme	POPC	1543:1546	arg1	monolayer					1548:1556	more loosely packed POPC monolayer	1523:1556	more loosely packed POPC monolayer	1523:1556	The nanoparticles adsorbed more easily into more loosely packed POPC monolayer.					
33671105	1	58	theme	aminated	238:245	arg1	chitosan					247:254	aminated chitosan	238:254	aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models	238:340	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	1	58	theme	aminated	238:245	arg1	Fe3O4-AChit					257:267	Fe3O4-AChit	257:267	Fe3O4-AChit	257:267	An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied.					
33671105	3	59	theme	regulation	641:650	arg1	existence					591:599	the existence	587:599	the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions	587:692	The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions.					
33671105	5	60	theme	penetration	1130:1140	arg1	experiments					1142:1152	penetration experiments	1130:1152	penetration experiments	1130:1152	The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction.					
33671105	0	61	with	Nanoparticles	41:53	arg1	Potential					74:82	Applicational Potential	60:82	Applicational Potential	60:82	Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.					
33671105	4	62	theme	nanoparticles	747:759	arg1	influence					699:707	The influence	695:707	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures	695:815	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
33671105	4	62	theme	nanoparticles	747:759	arg1	greater					825:831	greater	825:831	greater	825:831	The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC).					
32816991	3	0	contain	have	535:538	arg2	domains					558:564	similar catalytic domains	540:564	similar catalytic domains (CDs)	540:570	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	0	contain	have	535:538	arg1	cellobiohydrolase					469:485	cellobiohydrolase II	469:488	cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A)	469:533	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	0	contain	have	535:538	arg1	A					416:416	Cellobiohydrolase A	398:416	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B)	398:463	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	0	contain	have	535:538	arg2	CDs					567:569	CDs	567:569	CDs	567:569	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	7	1	theme	kinetic	1035:1041	arg1	parameters					1043:1052	kinetic parameters	1035:1052	kinetic parameters of CfCel6B	1035:1063	Here we analyze kinetic parameters of CfCel6B using single-molecule fluorescence imaging to compare CfCel6B and TrCel6A.					
32816991	4	2	theme	glycosylated	714:725	arg1	linker					735:740	a glycosylated peptide linker	712:740	a glycosylated peptide linker	712:740	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	3	theme	fibronectin	786:796	arg1	domains					805:811	three fibronectin type 3 domains	780:811	three fibronectin type 3 domains	780:811	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	6	4	theme	similar	1001:1007	arg1	function					1009:1016	similar function	1001:1016	similar function	1001:1016	However, it was not clear whether CfCel6B's linker has similar function.					
32816991	0	5	theme	catalytic	77:85	arg1	cellobiohydrolases					119:136	bacterial and fungal cellobiohydrolases	98:136	bacterial and fungal cellobiohydrolases	98:136	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	0	5	theme	catalytic	77:85	arg1	domains					87:93	catalytic domains	77:93	catalytic domains	77:93	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	5	6	theme	rate	906:909	arg1	constant					911:918	the binding rate constant	894:918	the binding rate constant to crystalline cellulose	894:943	We previously found that TrCel6A's linker plays an important role in increasing the binding rate constant to crystalline cellulose.					
32816991	3	7	theme	similar	540:546	arg1	CDs					567:569	CDs	567:569	CDs	567:569	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	7	theme	similar	540:546	arg1	domains					558:564	similar catalytic domains	540:564	similar catalytic domains (CDs)	540:570	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	10	8	theme	fast	1534:1537	arg1	diffusion					1547:1555	fast surface diffusion	1534:1555	fast surface diffusion	1534:1555	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	0	9	theme	bacterial	98:106	arg1	cellobiohydrolases					119:136	bacterial and fungal cellobiohydrolases	98:136	bacterial and fungal cellobiohydrolases	98:136	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	4	10	contain	has	708:710	arg1	TrCel6A					700:706	TrCel6A	700:706	TrCel6A	700:706	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	10	contain	has	708:710	arg2	linker					735:740	a glycosylated peptide linker	712:740	a glycosylated peptide linker	712:740	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	3	11	theme	Cellobiohydrolase	398:414	arg1	A					416:416	Cellobiohydrolase A	398:416	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B)	398:463	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	0	12	theme	Domain	0:5	arg1	divergence					20:29	Domain architecture divergence	0:29	Domain architecture divergence	0:29	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	10	13	theme	processive	1571:1580	arg1	movement					1582:1589	slow processive movement	1566:1589	slow processive movement	1566:1589	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	8	14	theme	rate	1289:1292	arg1	constant					1294:1301	the dissociation rate constant	1272:1301	the dissociation rate constant	1272:1301	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	0	15	theme	fungal	112:117	arg1	cellobiohydrolases					119:136	bacterial and fungal cellobiohydrolases	98:136	bacterial and fungal cellobiohydrolases	98:136	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	0	16	from	divergence	51:60	arg1	binding					65:71	binding	65:71	binding	65:71	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	0	16	from	divergence	51:60	arg1	domains					87:93	catalytic domains	77:93	catalytic domains	77:93	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	0	16	from	divergence	51:60	arg1	cellobiohydrolases					119:136	bacterial and fungal cellobiohydrolases	98:136	bacterial and fungal cellobiohydrolases	98:136	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	8	17	theme	rate	1252:1255	arg1	constant					1257:1264	the binding rate constant	1240:1264	the binding rate constant	1240:1264	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	4	18	theme	type	798:801	arg1	domains					805:811	three fibronectin type 3 domains	780:811	three fibronectin type 3 domains	780:811	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	10	19	theme	TrCel6A	1617:1623	arg1	CD					1625:1626	TrCel6A CD	1617:1626	TrCel6A CD	1617:1626	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	5	20	theme	important	865:873	arg1	role					875:878	an important role	862:878	an important role	862:878	We previously found that TrCel6A's linker plays an important role in increasing the binding rate constant to crystalline cellulose.					
32816991	8	21	theme	initial	1174:1180	arg1	binding					1182:1188	initial binding	1174:1188	initial binding of CfCel6B	1174:1199	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	11	22	theme	bacterial	1704:1712	arg1	cellobiohydrolases					1714:1731	bacterial cellobiohydrolases	1704:1731	bacterial cellobiohydrolases	1704:1731	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	3	23	from	reesei	518:523	arg1	cellobiohydrolase					469:485	cellobiohydrolase II	469:488	cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A)	469:533	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	23	from	reesei	518:523	arg1	A					416:416	Cellobiohydrolase A	398:416	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B)	398:463	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	2	24	theme	much	305:308	arg1	research					310:317	much research	305:317	much research in the field	305:330	As a result, much research in the field has focused on identifying cellobiohydrolases that are very fast.					
32816991	8	25	theme	linker	1230:1235	arg1	contribution					1210:1221	the contribution	1206:1221	the contribution of the linker to the binding rate constant or to the dissociation rate constant	1206:1301	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	8	25	theme	linker	1230:1235	arg1	minor					1306:1310	minor	1306:1310	minor	1306:1310	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	0	26	theme	architecture	7:18	arg1	divergence					20:29	Domain architecture divergence	0:29	Domain architecture divergence	0:29	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	10	27	located	observed	1605:1612	arg2	which					1592:1596	which	1592:1596	which	1592:1596	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	10	27	located	observed	1605:1612	arg1	CD					1625:1626	TrCel6A CD	1617:1626	TrCel6A CD	1617:1626	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	11	28	theme	high-processivity	1807:1823	arg1	CD					1825:1826	high-processivity CD	1807:1826	high-processivity CD	1807:1826	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	3	29	theme	hydrolytic	589:598	arg1	activity					600:607	similar hydrolytic activity	581:607	similar hydrolytic activity	581:607	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	8	30	theme	binding	1244:1250	arg1	constant					1257:1264	the binding rate constant	1240:1264	the binding rate constant	1240:1264	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	3	31	theme	bacterium	427:435	arg1	fimi					450:453	the bacterium Cellulomonas fimi	423:453	the bacterium Cellulomonas fimi (CfCel6B)	423:463	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	31	theme	bacterium	427:435	arg1	CfCel6B					456:462	CfCel6B	456:462	CfCel6B	456:462	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	9	32	theme	crystal	1317:1323	arg1	structure					1325:1333	The crystal structure	1313:1333	The crystal structure of the CfCel6B CD	1313:1351	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	11	33	theme	phylogenetic	1660:1671	arg1	analysis					1678:1685	a phylogenetic tree analysis	1658:1685	a phylogenetic tree analysis	1658:1685	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	10	34	theme	slow	1566:1569	arg1	movement					1582:1589	slow processive movement	1566:1589	slow processive movement	1566:1589	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	11	35	theme	high-affinity	1785:1797	arg1	CBD					1799:1801	high-affinity CBD	1785:1801	high-affinity CBD	1785:1801	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	7	36	theme	single-molecule	1071:1085	arg1	imaging					1100:1106	single-molecule fluorescence imaging	1071:1106	single-molecule fluorescence imaging	1071:1106	Here we analyze kinetic parameters of CfCel6B using single-molecule fluorescence imaging to compare CfCel6B and TrCel6A.					
32816991	6	37	contain	has	997:999	arg1	linker					990:995	CfCel6B's linker	980:995	CfCel6B's linker	980:995	However, it was not clear whether CfCel6B's linker has similar function.					
32816991	6	37	contain	has	997:999	arg2	function					1009:1016	similar function	1001:1016	similar function	1001:1016	However, it was not clear whether CfCel6B's linker has similar function.					
32816991	1	38	theme	biotechnological	224:239	arg1	interest					241:248	biotechnological interest	224:248	biotechnological interest	224:248	Cellobiohydrolases directly convert crystalline cellulose into cellobiose and are of biotechnological interest to achieve efficient biomass utilization.					
32816991	11	39	theme	crystalline	1757:1767	arg1	cellulose					1769:1777	crystalline cellulose	1757:1777	crystalline cellulose using high-affinity CBD and high-processivity CD	1757:1826	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	0	40	theme	cellobiohydrolases	119:136	arg1	binding					65:71	binding	65:71	binding	65:71	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	0	40	theme	cellobiohydrolases	119:136	arg1	domains					87:93	catalytic domains	77:93	catalytic domains	77:93	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	0	40	theme	cellobiohydrolases	119:136	arg1	cellobiohydrolases					119:136	bacterial and fungal cellobiohydrolases	98:136	bacterial and fungal cellobiohydrolases	98:136	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	3	41	theme	similar	581:587	arg1	activity					600:607	similar hydrolytic activity	581:607	similar hydrolytic activity	581:607	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	4	42	theme	cellulose-binding	654:670	arg1	CBDs					681:684	CBDs	681:684	CBDs	681:684	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	42	theme	cellulose-binding	654:670	arg1	domains					672:678	different cellulose-binding domains	644:678	different cellulose-binding domains (CBDs)	644:685	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	0	43	theme	functional	40:49	arg1	divergence					51:60	functional divergence	40:60	functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases	40:136	Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.					
32816991	5	44	theme	crystalline	923:933	arg1	cellulose					935:943	crystalline cellulose	923:943	crystalline cellulose	923:943	We previously found that TrCel6A's linker plays an important role in increasing the binding rate constant to crystalline cellulose.					
32816991	7	45	theme	CfCel6B	1057:1063	arg1	parameters					1043:1052	kinetic parameters	1035:1052	kinetic parameters of CfCel6B	1035:1063	Here we analyze kinetic parameters of CfCel6B using single-molecule fluorescence imaging to compare CfCel6B and TrCel6A.					
32816991	9	46	dep	entrance	1380:1387	arg1	the					1376:1378	the	1376:1378	the	1376:1378	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	9	47	theme	substrate-binding	1405:1421	arg1	tunnel					1423:1428	the substrate-binding tunnel	1401:1428	the substrate-binding tunnel	1401:1428	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	9	48	theme	CfCel6B	1342:1348	arg1	CD					1350:1351	the CfCel6B CD	1338:1351	the CfCel6B CD	1338:1351	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	3	49	from	fimi	450:453	arg1	cellobiohydrolase					469:485	cellobiohydrolase II	469:488	cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A)	469:533	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	49	from	fimi	450:453	arg1	A					416:416	Cellobiohydrolase A	398:416	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B)	398:463	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	8	50	theme	CfCel6B	1193:1199	arg1	binding					1182:1188	initial binding	1174:1188	initial binding of CfCel6B	1174:1199	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	3	51	theme	Cellulomonas	437:448	arg1	fimi					450:453	the bacterium Cellulomonas fimi	423:453	the bacterium Cellulomonas fimi (CfCel6B)	423:463	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	51	theme	Cellulomonas	437:448	arg1	CfCel6B					456:462	CfCel6B	456:462	CfCel6B	456:462	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	9	52	theme	CD	1350:1351	arg1	structure					1325:1333	The crystal structure	1313:1333	The crystal structure of the CfCel6B CD	1313:1351	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	10	53	theme	CfCel6B	1507:1513	arg1	CD					1515:1516	CfCel6B CD	1507:1516	CfCel6B CD	1507:1516	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	8	54	theme	dissociation	1276:1287	arg1	constant					1294:1301	the dissociation rate constant	1272:1301	the dissociation rate constant	1272:1301	We find that CBD is important for initial binding of CfCel6B, but the contribution of the linker to the binding rate constant or to the dissociation rate constant is minor.					
32816991	4	55	theme	peptide	727:733	arg1	linker					735:740	a glycosylated peptide linker	712:740	a glycosylated peptide linker	712:740	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	2	56	from	research	310:317	arg1	field					326:330	the field	322:330	the field	322:330	As a result, much research in the field has focused on identifying cellobiohydrolases that are very fast.					
32816991	9	57	theme	longer	1360:1365	arg1	loops					1367:1371	longer loops	1360:1371	longer loops	1360:1371	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	4	58	contain	have	639:642	arg1	CfCel6B					631:637	CfCel6B	631:637	CfCel6B	631:637	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	58	contain	have	639:642	arg1	TrCel6A					619:625	TrCel6A	619:625	TrCel6A	619:625	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	58	contain	have	639:642	arg2	CBDs					681:684	CBDs	681:684	CBDs	681:684	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	58	contain	have	639:642	arg2	linkers					691:697	linkers	691:697	linkers	691:697	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	58	contain	have	639:642	arg2	domains					672:678	different cellulose-binding domains	644:678	different cellulose-binding domains (CBDs)	644:685	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	9	59	theme	higher	1473:1478	arg1	processivity					1480:1491	higher processivity	1473:1491	higher processivity	1473:1491	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	1	60	theme	efficient	261:269	arg1	utilization					279:289	efficient biomass utilization	261:289	efficient biomass utilization	261:289	Cellobiohydrolases directly convert crystalline cellulose into cellobiose and are of biotechnological interest to achieve efficient biomass utilization.					
32816991	4	61	gly	glycosylated	714:725	arg1	linker					735:740	a glycosylated peptide linker	712:740	a glycosylated peptide linker	712:740	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	11	62	theme	tree	1673:1676	arg1	analysis					1678:1685	a phylogenetic tree analysis	1658:1685	a phylogenetic tree analysis	1658:1685	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	5	63	theme	binding	898:904	arg1	constant					911:918	the binding rate constant	894:918	the binding rate constant to crystalline cellulose	894:943	We previously found that TrCel6A's linker plays an important role in increasing the binding rate constant to crystalline cellulose.					
32816991	1	64	theme	biomass	271:277	arg1	utilization					279:289	efficient biomass utilization	261:289	efficient biomass utilization	261:289	Cellobiohydrolases directly convert crystalline cellulose into cellobiose and are of biotechnological interest to achieve efficient biomass utilization.					
32816991	11	65	theme	analysis	1678:1685	arg1	results					1647:1653	the results	1643:1653	the results of a phylogenetic tree analysis	1643:1685	Combined with the results of a phylogenetic tree analysis, we propose that bacterial cellobiohydrolases are designed to degrade crystalline cellulose using high-affinity CBD and high-processivity CD.					
32816991	9	66	theme	TrCel6A	1444:1450	arg1	CD					1452:1453	TrCel6A CD	1444:1453	TrCel6A CD	1444:1453	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	3	67	theme	catalytic	548:556	arg1	CDs					567:569	CDs	567:569	CDs	567:569	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	3	67	theme	catalytic	548:556	arg1	domains					558:564	similar catalytic domains	540:564	similar catalytic domains (CDs)	540:570	Cellobiohydrolase A from the bacterium Cellulomonas fimi (CfCel6B) and cellobiohydrolase II from the fungus Trichoderma reesei (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity.					
32816991	9	68	theme	tunnel	1423:1428	arg1	exit					1393:1396	exit	1393:1396	exit	1393:1396	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	9	68	theme	tunnel	1423:1428	arg1	entrance					1380:1387	entrance	1380:1387	entrance	1380:1387	The crystal structure of the CfCel6B CD showed longer loops at the entrance and exit of the substrate-binding tunnel compared with TrCel6A CD, which results in higher processivity.					
32816991	4	69	theme	different	644:652	arg1	CBDs					681:684	CBDs	681:684	CBDs	681:684	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	4	69	theme	different	644:652	arg1	domains					672:678	different cellulose-binding domains	644:678	different cellulose-binding domains (CBDs)	644:685	However, TrCel6A and CfCel6B have different cellulose-binding domains (CBDs) and linkers: TrCel6A has a glycosylated peptide linker, whereas CfCel6B's linker consists of three fibronectin type 3 domains.					
32816991	7	70	theme	fluorescence	1087:1098	arg1	imaging					1100:1106	single-molecule fluorescence imaging	1071:1106	single-molecule fluorescence imaging	1071:1106	Here we analyze kinetic parameters of CfCel6B using single-molecule fluorescence imaging to compare CfCel6B and TrCel6A.					
32816991	10	71	theme	surface	1539:1545	arg1	diffusion					1547:1555	fast surface diffusion	1534:1555	fast surface diffusion	1534:1555	Furthermore, CfCel6B CD showed not only fast surface diffusion but also slow processive movement, which is not observed in TrCel6A CD.					
32816991	1	72	theme	crystalline	175:185	arg1	cellulose					187:195	crystalline cellulose	175:195	crystalline cellulose	175:195	Cellobiohydrolases directly convert crystalline cellulose into cellobiose and are of biotechnological interest to achieve efficient biomass utilization.					
33022348	6	0	theme	arsenate	1219:1226	arg1	concentration					1202:1214	the normal concentration	1191:1214	the normal concentration of arsenate in natural water	1191:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	6	1	theme	chitosan-MNP	1246:1257	arg1	sample					1259:1264	chitosan-MNP sample	1246:1264	chitosan-MNP sample	1246:1264	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	5	2	theme	adsorption	879:888	arg1	capacity					890:897	higher adsorption capacity	872:897	higher adsorption capacity	872:897	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	3	3	theme	Langmuir	498:505	arg1	equation					507:514	Langmuir equation	498:514	Langmuir equation for the composite sample	498:539	The sorption of As (V) was reasonably explained using Freundlich isotherm for neat chitosan film although it was better represented by Langmuir equation for the composite sample.					
33022348	6	4	theme	chitosan	1373:1380	arg1	film					1382:1385	the chitosan film	1369:1385	the chitosan film	1369:1385	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	4	5	theme	first	802:806	arg1	model					825:829	a pseudo first order reversible model	793:829	a pseudo first order reversible model	793:829	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	2	6	theme	different	278:286	arg1	models					288:293	different models	278:293	different models applied to enlighten experimental observations and predict results	278:360	Sorption equilibrium and sorption kinetics studies are reported, including different models applied to enlighten experimental observations and predict results.					
33022348	5	7	theme	adsorbent	939:947	arg1	capacity					949:956	the additional adsorbent capacity	924:956	the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface	924:1063	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	6	8	theme	natural	1231:1237	arg1	water					1239:1243	natural water	1231:1243	natural water	1231:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	6	9	theme	10 mg/L	1083:1089	arg1	concentration					1103:1115	10 mg/L equilibrium concentration	1083:1115	10 mg/L equilibrium concentration	1083:1115	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	1	10	with	chitosan	115:122	arg1	nanoparticles					138:150	magnetic nanoparticles	129:150	magnetic nanoparticles (MNPs)	129:157	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	1	10	with	chitosan	115:122	arg1	MNPs					153:156	MNPs	153:156	MNPs	153:156	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	4	11	theme	order	808:812	arg1	model					825:829	a pseudo first order reversible model	793:829	a pseudo first order reversible model	793:829	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	3	12	theme	composite	524:532	arg1	sample					534:539	the composite sample	520:539	the composite sample	520:539	The sorption of As (V) was reasonably explained using Freundlich isotherm for neat chitosan film although it was better represented by Langmuir equation for the composite sample.					
33022348	4	13	theme	first	644:648	arg1	minutes					650:656	the first minutes	640:656	the first minutes	640:656	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	1	14	with	chitosan	102:109	arg1	nanoparticles					138:150	magnetic nanoparticles	129:150	magnetic nanoparticles (MNPs)	129:157	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	1	14	with	chitosan	102:109	arg1	MNPs					153:156	MNPs	153:156	MNPs	153:156	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	4	15	from	desorption	721:730	arg1	film					748:751	the chitosan film	735:751	the chitosan film	735:751	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	4	16	theme	slow	716:719	arg1	desorption					721:730	a slow desorption	714:730	a slow desorption in the chitosan film	714:751	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	5	17	theme	containing	846:855	arg1	MNPs					857:860	The adsorbent containing MNPs	832:860	The adsorbent containing MNPs	832:860	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	4	18	theme	experimental	546:557	arg1	results					568:574	The experimental kinetics results	542:574	The experimental kinetics results	542:574	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	4	19	theme	reversible	814:823	arg1	model					825:829	a pseudo first order reversible model	793:829	a pseudo first order reversible model	793:829	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	3	20	theme	Freundlich	417:426	arg1	isotherm					428:435	Freundlich isotherm	417:435	Freundlich isotherm for neat chitosan film	417:458	The sorption of As (V) was reasonably explained using Freundlich isotherm for neat chitosan film although it was better represented by Langmuir equation for the composite sample.					
33022348	6	21	theme	higher	1179:1184	arg1	concentration					1150:1162	an initial concentration	1139:1162	an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water	1139:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	5	22	theme	additional	928:937	arg1	capacity					949:956	the additional adsorbent capacity	924:956	the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface	924:1063	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	3	23	theme	As	379:380	arg1	sorption					367:374	The sorption	363:374	The sorption of As (V)	363:384	The sorption of As (V) was reasonably explained using Freundlich isotherm for neat chitosan film although it was better represented by Langmuir equation for the composite sample.					
33022348	6	24	theme	As	1167:1168	arg1	concentration					1150:1162	an initial concentration	1139:1162	an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water	1139:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	6	25	theme	10.4 mg/g	1297:1305	arg1	higher					1335:1340	higher	1335:1340	higher	1335:1340	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	6	25	theme	10.4 mg/g	1297:1305	arg1	capacity					1285:1292	a removal capacity	1275:1292	a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film	1275:1385	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	5	26	theme	enhanced	1037:1044	arg1	surface					1057:1063	an enhanced adsorption surface	1034:1063	an enhanced adsorption surface	1034:1063	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	1	27	theme	neat	97:100	arg1	chitosan					102:109	neat chitosan	97:109	neat chitosan	97:109	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	5	28	theme	surface	1007:1013	arg1	area					1015:1018	its much more irregular surface area	983:1018	its much more irregular surface area	983:1018	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	4	29	theme	kinetics	559:566	arg1	results					568:574	The experimental kinetics results	542:574	The experimental kinetics results	542:574	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	1	30	theme	arsenate	188:195	arg1	ions					197:200	arsenate ions	188:200	arsenate ions	188:200	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	6	31	theme	normal	1195:1200	arg1	concentration					1202:1214	the normal concentration	1191:1214	the normal concentration of arsenate in natural water	1191:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	3	32	theme	neat	441:444	arg1	chitosan					446:453	neat chitosan film	441:458	neat chitosan film	441:458	The sorption of As (V) was reasonably explained using Freundlich isotherm for neat chitosan film although it was better represented by Langmuir equation for the composite sample.					
33022348	0	33	theme	arsenic	14:20	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of arsenic onto films	0:31	Adsorption of arsenic onto films based on chitosan and chitosan/nano-iron oxide.					
33022348	6	34	from	concentration	1202:1214	arg1	water					1239:1243	natural water	1231:1243	natural water	1231:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	5	35	dep	MNPs	974:977	arg1	the					970:972	the	970:972	the	970:972	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	5	36	theme	irregular	997:1005	arg1	area					1015:1018	its much more irregular surface area	983:1018	its much more irregular surface area	983:1018	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	1	37	theme	ions	197:200	arg1	Films					81:85	Films	81:85	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs)	81:157	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	1	37	theme	ions	197:200	arg1	adsorbents					174:183	adsorbents	174:183	adsorbents of arsenate ions	174:200	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	6	38	theme	removal	1277:1283	arg1	higher					1335:1340	higher	1335:1340	higher	1335:1340	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	6	38	theme	removal	1277:1283	arg1	capacity					1285:1292	a removal capacity	1275:1292	a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film	1275:1385	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	2	39	theme	Sorption	203:210	arg1	equilibrium					212:222	Sorption equilibrium and sorption kinetics studies	203:252	equilibrium	212:222	Sorption equilibrium and sorption kinetics studies are reported, including different models applied to enlighten experimental observations and predict results.					
33022348	4	40	theme	pseudo	795:800	arg1	model					825:829	a pseudo first order reversible model	793:829	a pseudo first order reversible model	793:829	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	6	41	theme	equilibrium	1091:1101	arg1	concentration					1103:1115	10 mg/L equilibrium concentration	1083:1115	10 mg/L equilibrium concentration	1083:1115	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	4	42	theme	arsenate	606:613	arg1	ions					615:618	arsenate ions	606:618	arsenate ions	606:618	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	6	43	theme	initial	1142:1148	arg1	concentration					1150:1162	an initial concentration	1139:1162	an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water	1139:1243	For instance, at 10 mg/L equilibrium concentration, which corresponds to an initial concentration of As (V) much higher than the normal concentration of arsenate in natural water, chitosan-MNP sample exhibits a removal capacity of 10.4 mg/g that is more than six times higher than the 1.6 mg/g shown by the chitosan film.					
33022348	4	44	theme	chitosan	739:746	arg1	film					748:751	the chitosan film	735:751	the chitosan film	735:751	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	5	45	theme	adsorption	1046:1055	arg1	surface					1057:1063	an enhanced adsorption surface	1034:1063	an enhanced adsorption surface	1034:1063	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	2	46	theme	experimental	316:327	arg1	observations					329:340	experimental observations	316:340	experimental observations	316:340	Sorption equilibrium and sorption kinetics studies are reported, including different models applied to enlighten experimental observations and predict results.					
33022348	0	47	theme	chitosan/nano-iron	55:72	arg1	oxide					74:78	chitosan/nano-iron oxide	55:78	chitosan/nano-iron oxide	55:78	Adsorption of arsenic onto films based on chitosan and chitosan/nano-iron oxide.					
33022348	2	48	theme	kinetics	237:244	arg1	studies					246:252	Sorption equilibrium and sorption kinetics studies	203:252	studies	246:252	Sorption equilibrium and sorption kinetics studies are reported, including different models applied to enlighten experimental observations and predict results.					
33022348	1	49	theme	magnetic	129:136	arg1	nanoparticles					138:150	magnetic nanoparticles	129:150	magnetic nanoparticles (MNPs)	129:157	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	1	49	theme	magnetic	129:136	arg1	MNPs					153:156	MNPs	153:156	MNPs	153:156	Films made from neat chitosan and chitosan with magnetic nanoparticles (MNPs) were tested as adsorbents of arsenate ions.					
33022348	5	50	theme	adsorbent	836:844	arg1	MNPs					857:860	The adsorbent containing MNPs	832:860	The adsorbent containing MNPs	832:860	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	2	51	theme	sorption	228:235	arg1	studies					246:252	Sorption equilibrium and sorption kinetics studies	203:252	studies	246:252	Sorption equilibrium and sorption kinetics studies are reported, including different models applied to enlighten experimental observations and predict results.					
33022348	5	52	theme	higher	872:877	arg1	capacity					890:897	higher adsorption capacity	872:897	higher adsorption capacity	872:897	The adsorbent containing MNPs presented higher adsorption capacity, which was associated to the additional adsorbent capacity provided by the MNPs and its much more irregular surface area that leads to an enhanced adsorption surface.					
33022348	4	53	theme	ions	615:618	arg1	adsorption					592:601	the adsorption	588:601	the adsorption of arsenate ions	588:618	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33022348	4	53	theme	ions	615:618	arg1	fast					628:631	fast	628:631	fast	628:631	The experimental kinetics results showed that the adsorption of arsenate ions is very fast during the first minutes and then the composite seems to reach saturation, while a slow desorption in the chitosan film was observed and acceptably fitted with a pseudo first order reversible model.					
33483055	2	0	used	used	225:228	arg2	complexation					208:219	electrostatic complexation	194:219	electrostatic complexation	194:219	Herein, electrostatic complexation was used to prepare wet-resilient foams with improved mechanical property.					
33483055	4	1	from	behavior	597:604	arg1	water					617:621	water	617:621	water	617:621	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	5	2	theme	attractive	767:776	arg1	interactions					778:789	meaningful attractive interactions	756:789	meaningful attractive interactions	756:789	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	1	3	theme	advanced	166:173	arg1	materials					175:183	advanced materials	166:183	advanced materials	166:183	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	6	4	theme	water	991:995	arg1	stability					997:1005	the water stability	987:1005	the water stability of cationic foams	987:1023	Besides the electrostatic attraction, hydrogen bonds and hydrophobic contacts may also occur within the clustering, improving the water stability of cationic foams.					
33483055	6	5	theme	hydrogen	899:906	arg1	bonds					908:912	hydrogen bonds	899:912	hydrogen bonds	899:912	Besides the electrostatic attraction, hydrogen bonds and hydrophobic contacts may also occur within the clustering, improving the water stability of cationic foams.					
33483055	5	6	theme	ca	726:727	arg1	1:1					721:723	1:1	721:723	1:1 (ca 0.12 MPa)	721:737	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	5	6	theme	ca	726:727	arg1	0.12 MPa					729:736	ca 0.12 MPa	726:736	ca 0.12 MPa	726:736	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	3	7	theme	cellulose	427:435	arg1	nanofibers					437:446	oppositely charged cellulose nanofibers	408:446	oppositely charged cellulose nanofibers	408:446	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	4	8	theme	rheological	585:595	arg1	behavior					597:604	the rheological behavior	581:604	the rheological behavior	581:604	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	7	9	theme	cellulose	1095:1103	arg1	materials					1105:1113	robust all- cellulose materials	1083:1113	robust all- cellulose materials prepared in water	1083:1131	These results may provide a basis for the development of robust all- cellulose materials prepared in water, with nontoxic chemicals.					
33483055	1	10	theme	Supramolecular	73:86	arg1	assembly					88:95	Supramolecular assembly	73:95	Supramolecular assembly of biobased components in water	73:127	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	1	10	theme	Supramolecular	73:86	arg1	strategy					144:151	a promising strategy	132:151	a promising strategy to construct advanced materials	132:183	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	4	11	from	stability	567:575	arg1	water					617:621	water	617:621	water	617:621	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	5	12	theme	mass	701:704	arg1	composition					706:716	the mass composition	697:716	the mass composition of 1:1 (ca 0.12 MPa)	697:737	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	3	13	theme	charged	419:425	arg1	nanofibers					437:446	oppositely charged cellulose nanofibers	408:446	oppositely charged cellulose nanofibers	408:446	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	4	14	from	water	617:621	arg1	stability					567:575	the colloidal stability	553:575	the colloidal stability	553:575	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	4	14	from	water	617:621	arg1	behavior					597:604	the rheological behavior	581:604	the rheological behavior	581:604	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	7	15	theme	materials	1105:1113	arg1	development					1068:1078	the development	1064:1078	the development of robust all- cellulose materials prepared in water	1064:1131	These results may provide a basis for the development of robust all- cellulose materials prepared in water, with nontoxic chemicals.					
33483055	4	16	theme	colloidal	557:565	arg1	stability					567:575	the colloidal stability	553:575	the colloidal stability	553:575	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	5	17	theme	1:1	721:723	arg1	composition					706:716	the mass composition	697:716	the mass composition of 1:1 (ca 0.12 MPa)	697:737	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	1	18	theme	biobased	100:107	arg1	components					109:118	biobased components	100:118	biobased components	100:118	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	3	19	theme	entangled	490:498	arg1	fibers					500:505	entangled fibers	490:505	entangled fibers	490:505	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	0	20	theme	Tailoring	0:8	arg1	strength					10:17	Tailoring strength	0:17	Tailoring strength of nanocellulose	0:34	Tailoring strength of nanocellulose foams by electrostatic complexation.					
33483055	5	21	theme	maximum	666:672	arg1	modulus					686:692	maximum compressive modulus	666:692	maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa)	666:737	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	1	22	theme	components	109:118	arg1	assembly					88:95	Supramolecular assembly	73:95	Supramolecular assembly of biobased components in water	73:127	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	1	22	theme	components	109:118	arg1	strategy					144:151	a promising strategy	132:151	a promising strategy to construct advanced materials	132:183	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	3	23	theme	fibers	500:505	arg1	networks					478:485	networks	478:485	networks	478:485	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	3	23	theme	fibers	500:505	arg1	clusters					465:472	clusters	465:472	clusters	465:472	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	0	24	theme	nanocellulose	22:34	arg1	strength					10:17	Tailoring strength	0:17	Tailoring strength of nanocellulose	0:34	Tailoring strength of nanocellulose foams by electrostatic complexation.					
33483055	3	25	with	suspensions	391:401	arg1	nanofibers					437:446	oppositely charged cellulose nanofibers	408:446	oppositely charged cellulose nanofibers	408:446	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	3	26	theme	scattering	314:323	arg1	experiments					367:377	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments	296:377	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments	296:377	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	5	27	theme	compressive	674:684	arg1	modulus					686:692	maximum compressive modulus	666:692	maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa)	666:737	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	5	28	from	composition	706:716	arg1	modulus					686:692	maximum compressive modulus	666:692	maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa)	666:737	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	2	29	theme	electrostatic	194:206	arg1	complexation					208:219	electrostatic complexation	194:219	electrostatic complexation	194:219	Herein, electrostatic complexation was used to prepare wet-resilient foams with improved mechanical property.					
33483055	3	30	theme	networks	478:485	arg1	mixture					454:460	a mixture	452:460	a mixture of clusters and networks of entangled fibers	452:505	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	3	30	theme	networks	478:485	arg1	suspensions					391:401	suspensions	391:401	suspensions with oppositely charged cellulose nanofibers	391:446	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	3	31	theme	Small-angle	296:306	arg1	scattering					314:323	Small-angle X-ray scattering	296:323	Small-angle X-ray scattering	296:323	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	4	32	theme	CNFs	609:612	arg1	stability					567:575	the colloidal stability	553:575	the colloidal stability	553:575	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	4	32	theme	CNFs	609:612	arg1	behavior					597:604	the rheological behavior	581:604	the rheological behavior	581:604	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	3	33	theme	X-ray	308:312	arg1	scattering					314:323	Small-angle X-ray scattering	296:323	Small-angle X-ray scattering	296:323	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	7	34	theme	all-	1090:1093	arg1	materials					1105:1113	robust all- cellulose materials	1083:1113	robust all- cellulose materials prepared in water	1083:1131	These results may provide a basis for the development of robust all- cellulose materials prepared in water, with nontoxic chemicals.					
33483055	3	35	theme	clusters	465:472	arg1	mixture					454:460	a mixture	452:460	a mixture of clusters and networks of entangled fibers	452:505	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	3	35	theme	clusters	465:472	arg1	suspensions					391:401	suspensions	391:401	suspensions with oppositely charged cellulose nanofibers	391:446	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	3	36	theme	microscopy	356:365	arg1	experiments					367:377	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments	296:377	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments	296:377	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	2	37	theme	wet-resilient	241:253	arg1	foams					255:259	wet-resilient foams	241:259	wet-resilient foams	241:259	Herein, electrostatic complexation was used to prepare wet-resilient foams with improved mechanical property.					
33483055	5	38	theme	meaningful	756:765	arg1	interactions					778:789	meaningful attractive interactions	756:789	meaningful attractive interactions	756:789	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	0	39	theme	electrostatic	45:57	arg1	complexation					59:70	electrostatic complexation	45:70	electrostatic complexation	45:70	Tailoring strength of nanocellulose foams by electrostatic complexation.					
33483055	6	40	theme	foams	1019:1023	arg1	stability					997:1005	the water stability	987:1005	the water stability of cationic foams	987:1023	Besides the electrostatic attraction, hydrogen bonds and hydrophobic contacts may also occur within the clustering, improving the water stability of cationic foams.					
33483055	7	41	theme	nontoxic	1139:1146	arg1	chemicals					1148:1156	nontoxic chemicals	1139:1156	nontoxic chemicals	1139:1156	These results may provide a basis for the development of robust all- cellulose materials prepared in water, with nontoxic chemicals.					
33483055	6	42	theme	cationic	1010:1017	arg1	foams					1019:1023	cationic foams	1010:1023	cationic foams	1010:1023	Besides the electrostatic attraction, hydrogen bonds and hydrophobic contacts may also occur within the clustering, improving the water stability of cationic foams.					
33483055	3	43	theme	cryo-transmission	329:345	arg1	microscopy					356:365	cryo-transmission electron microscopy	329:365	cryo-transmission electron microscopy	329:365	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	6	44	theme	hydrophobic	918:928	arg1	contacts					930:937	hydrophobic contacts	918:937	hydrophobic contacts	918:937	Besides the electrostatic attraction, hydrogen bonds and hydrophobic contacts may also occur within the clustering, improving the water stability of cationic foams.					
33483055	7	45	theme	robust	1083:1088	arg1	materials					1105:1113	robust all- cellulose materials	1083:1113	robust all- cellulose materials prepared in water	1083:1131	These results may provide a basis for the development of robust all- cellulose materials prepared in water, with nontoxic chemicals.					
33483055	1	46	theme	promising	134:142	arg1	assembly					88:95	Supramolecular assembly	73:95	Supramolecular assembly of biobased components in water	73:127	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	1	46	theme	promising	134:142	arg1	strategy					144:151	a promising strategy	132:151	a promising strategy to construct advanced materials	132:183	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
33483055	3	47	theme	electron	347:354	arg1	microscopy					356:365	cryo-transmission electron microscopy	329:365	cryo-transmission electron microscopy	329:365	Small-angle X-ray scattering and cryo-transmission electron microscopy experiments showed that suspensions with oppositely charged cellulose nanofibers are a mixture of clusters and networks of entangled fibers.					
33483055	6	48	theme	electrostatic	873:885	arg1	attraction					887:896	the electrostatic attraction	869:896	the electrostatic attraction	869:896	Besides the electrostatic attraction, hydrogen bonds and hydrophobic contacts may also occur within the clustering, improving the water stability of cationic foams.					
33483055	5	49	theme	stiffening	823:832	arg1	structure					834:842	stiffening structure	823:842	stiffening structure in the material	823:858	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	5	50	from	structure	834:842	arg1	material					851:858	the material	847:858	the material	847:858	Foams prepared from suspensions exhibited maximum compressive modulus at the mass composition of 1:1 (ca 0.12 MPa), suggesting that meaningful attractive interactions happen at this point and act as stiffening structure in the material.					
33483055	2	51	theme	mechanical	275:284	arg1	property					286:293	improved mechanical property	266:293	improved mechanical property	266:293	Herein, electrostatic complexation was used to prepare wet-resilient foams with improved mechanical property.					
33483055	4	52	from	CNFs	609:612	arg1	water					617:621	water	617:621	water	617:621	The balance between these structures governs the colloidal stability and the rheological behavior of CNFs in water.					
33483055	2	53	theme	improved	266:273	arg1	property					286:293	improved mechanical property	266:293	improved mechanical property	266:293	Herein, electrostatic complexation was used to prepare wet-resilient foams with improved mechanical property.					
33483055	1	54	from	assembly	88:95	arg1	water					123:127	water	123:127	water	123:127	Supramolecular assembly of biobased components in water is a promising strategy to construct advanced materials.					
34029901	0	0	theme	morphological	65:77	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	0	1	from	effect	14:19	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	3	2	theme	protein	785:791	arg1	matrix					793:798	dense protein matrix	779:798	dense protein matrix	779:798	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	1	3	dep	flour	301:305	arg1	extracts					388:395	extracts	388:395	extracts	388:395	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	6	4	from	utilization	1285:1295	arg1	development					1308:1318	product development	1300:1318	product development	1300:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	1	5	theme	pasting	239:245	arg1	properties					275:284	pasting, rheological, morphological properties	239:284	pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts	239:395	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	3	6	theme	starch	749:754	arg1	structure					736:744	the continuous composite structure	711:744	the continuous composite structure of starch	711:754	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	2	7	from	improvement	470:480	arg1	intensity					491:499	shear intensity	485:499	shear intensity	485:499	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	4	8	theme	metal	907:911	arg1	ability					923:929	metal chelating ability	907:929	metal chelating ability	907:929	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	4	9	theme	chelating	913:921	arg1	ability					923:929	metal chelating ability	907:929	metal chelating ability	907:929	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	0	10	theme	in-	83:85	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	1	11	theme	phenolic	354:361	arg1	acid					383:386	its phenolic and γ-amino butyric acid	350:386	its phenolic and γ-amino butyric acid	350:386	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	4	12	theme	ability	923:929	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	4	13	theme	scavenging	953:962	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	0	14	theme	antioxidant	93:103	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	1	15	theme	γ-amino	367:373	arg1	acid					383:386	its phenolic and γ-amino butyric acid	350:386	its phenolic and γ-amino butyric acid	350:386	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	6	16	dep	compounds	1256:1264	arg1	extraction					1266:1275	extraction	1266:1275	extraction	1266:1275	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	16	dep	compounds	1256:1264	arg1	utilization					1285:1295	its utilization	1281:1295	its utilization in product development	1281:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	16	dep	compounds	1256:1264	arg1	compounds					1256:1264	valuable bioactive compounds extraction and its utilization in product development	1237:1318	valuable bioactive compounds extraction and its utilization in product development	1237:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	0	17	dep	in-	83:85	arg1	vitro					87:91	vitro	87:91	vitro	87:91	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	6	18	theme	product	1300:1306	arg1	development					1308:1318	product development	1300:1318	product development	1300:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	1	19	theme	butyric	375:381	arg1	acid					383:386	its phenolic and γ-amino butyric acid	350:386	its phenolic and γ-amino butyric acid	350:386	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	4	20	theme	*	898:898	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	4	21	theme	extracts	987:994	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	1	22	theme	present	160:166	arg1	investigationwas					168:183	The present investigationwas	156:183	The present investigationwas	156:183	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	1	23	theme	acid	383:386	arg1	flour					301:305	Kodo millet flour	289:305	Kodo millet flour	289:305	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	1	23	theme	acid	383:386	arg1	characteristics					331:345	in-vitroantioxidant characteristics	311:345	in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid	311:386	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	3	24	from	destruction	696:706	arg1	structure					736:744	the continuous composite structure	711:744	the continuous composite structure of starch	711:754	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	6	25	theme	bioactive	1246:1254	arg1	extraction					1266:1275	extraction	1266:1275	extraction	1266:1275	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	25	theme	bioactive	1246:1254	arg1	utilization					1285:1295	its utilization	1281:1295	its utilization in product development	1281:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	25	theme	bioactive	1246:1254	arg1	compounds					1256:1264	valuable bioactive compounds extraction and its utilization in product development	1237:1318	valuable bioactive compounds extraction and its utilization in product development	1237:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	4	26	theme	hydrogen	935:942	arg1	peroxide					944:951	hydrogen peroxide	935:951	hydrogen peroxide	935:951	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	3	27	theme	composite	726:734	arg1	structure					736:744	the continuous composite structure	711:744	the continuous composite structure of starch	711:754	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	4	28	theme	FRAP	901:904	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	5	29	theme	solid	1116:1120	arg1	behaviour					1122:1130	visco-elastic solid behaviour	1102:1130	visco-elastic solid behaviour	1102:1130	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
34029901	3	30	from	decrease	618:625	arg1	visco-elasticity					630:645	visco-elasticity	630:645	visco-elasticity	630:645	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	3	31	theme	temperature	585:595	arg1	sweep					597:601	temperature sweep	585:601	temperature sweep	585:601	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	6	32	from	extraction	1266:1275	arg1	development					1308:1318	product development	1300:1318	product development	1300:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	33	theme	valuable	1237:1244	arg1	extraction					1266:1275	extraction	1266:1275	extraction	1266:1275	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	33	theme	valuable	1237:1244	arg1	utilization					1285:1295	its utilization	1281:1295	its utilization in product development	1281:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	6	33	theme	valuable	1237:1244	arg1	compounds					1256:1264	valuable bioactive compounds extraction and its utilization in product development	1237:1318	valuable bioactive compounds extraction and its utilization in product development	1237:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	3	34	theme	dense	779:783	arg1	matrix					793:798	dense protein matrix	779:798	dense protein matrix	779:798	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	4	35	theme	in-vitroantioxidant	827:845	arg1	activities					847:856	The in-vitroantioxidant activities	823:856	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	823:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	4	35	theme	in-vitroantioxidant	827:845	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	5	36	theme	visco-elastic	1143:1155	arg1	fluid					1157:1161	visco-elastic fluid	1143:1161	visco-elastic fluid	1143:1161	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
34029901	6	37	theme	flour	1227:1231	arg1	potential					1191:1199	the potential	1187:1199	the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development	1187:1318	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	2	38	theme	flour	436:440	arg1	viscosity					442:450	complex flour viscosity	428:450	complex flour viscosity	428:450	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	1	39	theme	Kodo	289:292	arg1	flour					301:305	Kodo millet flour	289:305	Kodo millet flour	289:305	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	0	40	theme	millet	129:134	arg1	flour					136:140	kodo millet flour	124:140	kodo millet flour	124:140	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	4	41	theme	capacity	884:891	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	2	42	theme	complex	428:434	arg1	viscosity					442:450	complex flour viscosity	428:450	complex flour viscosity	428:450	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	0	43	theme	germination	24:34	arg1	effect					14:19	the effect	10:19	the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	10:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	1	44	theme	millet	294:299	arg1	flour					301:305	Kodo millet flour	289:305	Kodo millet flour	289:305	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	0	45	theme	kodo	124:127	arg1	flour					136:140	kodo millet flour	124:140	kodo millet flour	124:140	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	3	46	theme	wherein	674:680	arg1	result					652:657	a result	650:657	a result of germination wherein, SEM	650:685	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	3	46	theme	wherein	674:680	arg1	decrease					618:625	a decrease	616:625	a decrease in visco-elasticity	616:645	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	4	47	theme	antioxidant	872:882	arg1	capacity					884:891	total antioxidant capacity	866:891	total antioxidant capacity	866:891	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	0	48	theme	pasting	43:49	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	1	49	theme	flour	301:305	arg1	properties					275:284	pasting, rheological, morphological properties	239:284	pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts	239:395	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	4	50	theme	peroxide	944:951	arg1	activities					964:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities	866:973	total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts	866:994	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	2	51	theme	shear-thinning	518:531	arg1	action					533:538	the shear-thinning action	514:538	the shear-thinning action of flour upon germination	514:564	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	4	52	theme	DPPH	894:897	arg1	*					898:898	DPPH*	894:898	DPPH*	894:898	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	6	53	theme	millet	1220:1225	arg1	flour					1227:1231	germinated Kodo millet flour	1204:1231	germinated Kodo millet flour	1204:1231	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	0	54	theme	flour	136:140	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	2	55	theme	flour	543:547	arg1	action					533:538	the shear-thinning action	514:538	the shear-thinning action of flour upon germination	514:564	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	5	56	theme	visco-elastic	1102:1114	arg1	behaviour					1122:1130	visco-elastic solid behaviour	1102:1130	visco-elastic solid behaviour	1102:1130	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
34029901	6	57	theme	Kodo	1215:1218	arg1	flour					1227:1231	germinated Kodo millet flour	1204:1231	germinated Kodo millet flour	1204:1231	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	1	58	theme	in-vitroantioxidant	311:329	arg1	characteristics					331:345	in-vitroantioxidant characteristics	311:345	in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid	311:386	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	1	59	from	effect	214:219	arg1	properties					275:284	pasting, rheological, morphological properties	239:284	pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts	239:395	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	3	60	theme	continuous	715:724	arg1	structure					736:744	the continuous composite structure	711:744	the continuous composite structure of starch	711:754	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	5	61	theme	pasting	1045:1051	arg1	properties					1053:1062	pasting properties	1045:1062	pasting properties	1045:1062	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
34029901	3	62	dep	frequency	571:579	arg1	The					567:569	The	567:569	The	567:569	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	0	63	theme	extracts	146:153	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	1	64	dep	pasting	239:245	arg1	morphological					261:273	morphological	261:273	morphological	261:273	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	1	64	dep	pasting	239:245	arg1	rheological					248:258	rheological	248:258	rheological	248:258	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	3	65	theme	germination	662:672	arg1	SEM					683:685	SEM	683:685	SEM	683:685	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	3	65	theme	germination	662:672	arg1	wherein					674:680	germination wherein	662:680	germination wherein	662:680	The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination.					
34029901	6	66	theme	germinated	1204:1213	arg1	flour					1227:1231	germinated Kodo millet flour	1204:1231	germinated Kodo millet flour	1204:1231	This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.					
34029901	0	67	theme	rheological	52:62	arg1	characteristics					105:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics	39:119	the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts	39:153	Analyzing the effect of germination on the pasting, rheological, morphological and in- vitro antioxidant characteristics of kodo millet flour and extracts.					
34029901	4	68	theme	total	866:870	arg1	capacity					884:891	total antioxidant capacity	866:891	total antioxidant capacity	866:891	The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly.					
34029901	2	69	theme	Rheological	398:408	arg1	analysis					410:417	Rheological analysis	398:417	Rheological analysis	398:417	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	1	70	theme	germination	224:234	arg1	effect					214:219	the effect	210:219	the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts	210:395	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	2	71	theme	shear	485:489	arg1	intensity					491:499	shear intensity	485:499	shear intensity	485:499	Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination.					
34029901	1	72	theme	characteristics	331:345	arg1	properties					275:284	pasting, rheological, morphological properties	239:284	pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts	239:395	The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts.					
34029901	5	73	theme	gelatinization	1068:1081	arg1	behaviour					1083:1091	gelatinization behaviour	1068:1091	gelatinization behaviour	1068:1091	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
34029901	5	74	from	decrease	1033:1040	arg1	properties					1053:1062	pasting properties	1045:1062	pasting properties	1045:1062	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
34029901	5	74	from	decrease	1033:1040	arg1	behaviour					1083:1091	gelatinization behaviour	1068:1091	gelatinization behaviour	1068:1091	There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid.					
32434160	9	0	theme	zonal	1577:1581	arg1	constructs					1583:1592	zonal constructs	1577:1592	zonal constructs	1577:1592	There were no differences in histological scoring; however, the repair tissue was significantly stiffer in defects repaired with zonal constructs.					
32434160	1	1	theme	osteochondral	242:254	arg1	constructs					256:265	personalized osteochondral constructs	229:265	personalized osteochondral constructs for joint repair	229:282	Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair.					
32434160	9	2	from	differences	1462:1472	arg1	scoring					1490:1496	histological scoring	1477:1496	histological scoring	1477:1496	There were no differences in histological scoring; however, the repair tissue was significantly stiffer in defects repaired with zonal constructs.					
32434160	10	3	theme	early	1685:1689	arg1	loss					1691:1694	early loss	1685:1694	early loss of implanted cells	1685:1713	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	4	4	theme	construct	791:799	arg1	part					779:782	the chondral part	766:782	the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	766:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	3	5	theme	composite	503:511	arg1	implant					513:519	a composite implant	501:519	a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model	501:640	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	1	6	theme	joint	271:275	arg1	repair					277:282	joint repair	271:282	joint repair	271:282	Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair.					
32434160	10	7	theme	inappropriate	1719:1731	arg1	rate					1745:1748	inappropriate degradation rate	1719:1748	inappropriate degradation rate of the hydrogel	1719:1764	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	3	8	theme	zonal	547:551	arg1	distribution					553:564	the zonal distribution	543:564	the zonal distribution of cellular component	543:586	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	4	9	theme	glycidol	874:881	arg1	HA-SH/P					901:907	HA-SH/P	901:907	HA-SH/P(AGE-co-G)	901:917	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	9	theme	glycidol	874:881	arg1	hydrogel					891:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel	826:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	826:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	2	10	theme	articular	292:300	arg1	cartilage					302:310	Native articular cartilage	285:310	Native articular cartilage	285:310	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	4	11	theme	hybrid	884:889	arg1	HA-SH/P					901:907	HA-SH/P	901:907	HA-SH/P(AGE-co-G)	901:917	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	11	theme	hybrid	884:889	arg1	hydrogel					891:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel	826:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	826:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	6	12	theme	constructs	1159:1168	arg1	part					1143:1146	The chondral part	1130:1146	The chondral part of control constructs	1130:1168	The chondral part of control constructs were homogeneously filled with MSCs.					
32434160	8	13	dep	constructs	1436:1445	arg1	both					1408:1411	both	1408:1411	both	1408:1411	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	10	14	theme	degradation	1733:1743	arg1	rate					1745:1748	inappropriate degradation rate	1719:1748	inappropriate degradation rate of the hydrogel	1719:1764	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	4	15	theme	cross-linkable	838:851	arg1	HA-SH/P					901:907	HA-SH/P	901:907	HA-SH/P(AGE-co-G)	901:917	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	15	theme	cross-linkable	838:851	arg1	hydrogel					891:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel	826:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	826:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	16	theme	poly	682:685	arg1	anchor					714:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor	669:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	669:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	5	17	theme	Progenitor	981:990	arg1	ACPCs					999:1003	ACPCs	999:1003	ACPCs	999:1003	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	17	theme	Progenitor	981:990	arg1	Cells					992:996	Articular Cartilage Progenitor Cells	961:996	Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs)	961:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	11	18	theme	characteristics	1880:1894	arg1	research					1815:1822	research	1815:1822	research into further tailoring of biomaterials and of construct characteristics	1815:1894	Nonetheless, this approach may be promising and research into further tailoring of biomaterials and of construct characteristics seems warranted.					
32434160	4	19	theme	acid/poly	864:872	arg1	HA-SH/P					901:907	HA-SH/P	901:907	HA-SH/P(AGE-co-G)	901:917	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	19	theme	acid/poly	864:872	arg1	hydrogel					891:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel	826:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	826:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	6	20	theme	chondral	1134:1141	arg1	part					1143:1146	The chondral part	1130:1146	The chondral part of control constructs	1130:1168	The chondral part of control constructs were homogeneously filled with MSCs.					
32434160	4	21	theme	ϵ-caprolactone	687:700	arg1	anchor					714:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor	669:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	669:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	0	22	theme	long-term	143:151	arg1	model					160:164	a long-term equine model	141:164	a long-term equine model	141:164	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	3	23	theme	implant	513:519	arg1	performance					486:496	the performance	482:496	the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model	482:640	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	5	24	theme	cartilage	1051:1059	arg1	tissue					1061:1066	native cartilage tissue	1044:1066	native cartilage tissue	1044:1066	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	10	25	theme	hydrogel	1757:1764	arg1	loss					1691:1694	early loss	1685:1694	early loss of implanted cells	1685:1713	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	25	theme	hydrogel	1757:1764	arg1	rate					1745:1748	inappropriate degradation rate	1719:1748	inappropriate degradation rate of the hydrogel	1719:1764	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	5	26	theme	superficial	1023:1033	arg1	layer					1035:1039	the superficial layer	1019:1039	the superficial layer of native cartilage tissue	1019:1066	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	26	theme	superficial	1023:1033	arg1	layer					1080:1084	the bottom layer	1069:1084	the bottom layer contained mesenchymal stromal cells (MSCs)	1069:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	9	27	theme	histological	1477:1488	arg1	scoring					1490:1496	histological scoring	1477:1496	histological scoring	1477:1496	There were no differences in histological scoring; however, the repair tissue was significantly stiffer in defects repaired with zonal constructs.					
32434160	11	28	theme	further	1829:1835	arg1	tailoring					1837:1845	further tailoring	1829:1845	further tailoring of biomaterials	1829:1861	Nonetheless, this approach may be promising and research into further tailoring of biomaterials and of construct characteristics seems warranted.					
32434160	0	29	theme	cartilage	100:108	arg1	repair					110:115	cartilage repair	100:115	cartilage repair	100:115	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	7	30	theme	bone	1270:1273	arg1	growth					1275:1280	significant bone growth	1258:1280	significant bone growth	1258:1280	After six months in vivo, microtomography revealed significant bone growth into the anchor.					
32434160	2	31	theme	failure	421:427	arg1	which					384:388	which	384:388	which	384:388	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	2	31	theme	failure	421:427	arg1	cause					399:403	a cause	397:403	a cause of the frequent failure of these repair attempts	397:452	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	11	32	theme	biomaterials	1850:1861	arg1	tailoring					1837:1845	further tailoring	1829:1845	further tailoring of biomaterials	1829:1861	Nonetheless, this approach may be promising and research into further tailoring of biomaterials and of construct characteristics seems warranted.					
32434160	5	33	theme	stromal	1108:1114	arg1	cells					1116:1120	mesenchymal stromal cells	1096:1120	mesenchymal stromal cells (MSCs)	1096:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	33	theme	stromal	1108:1114	arg1	MSCs					1123:1126	MSCs	1123:1126	MSCs	1123:1126	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	9	34	theme	repair	1512:1517	arg1	tissue					1519:1524	the repair tissue	1508:1524	the repair tissue	1508:1524	There were no differences in histological scoring; however, the repair tissue was significantly stiffer in defects repaired with zonal constructs.					
32434160	9	34	theme	repair	1512:1517	arg1	stiffer					1544:1550	stiffer	1544:1550	stiffer	1544:1550	There were no differences in histological scoring; however, the repair tissue was significantly stiffer in defects repaired with zonal constructs.					
32434160	2	35	theme	attempts	445:452	arg1	failure					421:427	the frequent failure	408:427	the frequent failure of these repair attempts	408:452	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	4	36	theme	chondral	770:777	arg1	part					779:782	the chondral part	766:782	the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	766:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	5	37	theme	bottom	1073:1078	arg1	layer					1080:1084	the bottom layer	1069:1084	the bottom layer contained mesenchymal stromal cells (MSCs)	1069:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	37	theme	bottom	1073:1078	arg1	layer					1035:1039	the superficial layer	1019:1039	the superficial layer of native cartilage tissue	1019:1066	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	7	38	from	months	1217:1222	arg1	vivo					1227:1230	vivo	1227:1230	vivo	1227:1230	After six months in vivo, microtomography revealed significant bone growth into the anchor.					
32434160	2	39	contain	has	312:314	arg1	cartilage					302:310	Native articular cartilage	285:310	Native articular cartilage	285:310	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	2	39	contain	has	312:314	arg2	structure					324:332	a zonal structure	316:332	a zonal structure	316:332	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	5	40	theme	Cartilage	971:979	arg1	ACPCs					999:1003	ACPCs	999:1003	ACPCs	999:1003	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	40	theme	Cartilage	971:979	arg1	Cells					992:996	Articular Cartilage Progenitor Cells	961:996	Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs)	961:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	8	41	theme	cartilage-like	1352:1365	arg1	tissue					1367:1372	cartilage-like tissue	1352:1372	cartilage-like tissue	1352:1372	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	10	42	theme	tissue	1633:1638	arg1	quality					1611:1617	The sub-optimal quality	1595:1617	The sub-optimal quality of the repair tissue	1595:1638	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	42	theme	tissue	1633:1638	arg1	related					1647:1653	related	1647:1653	related	1647:1653	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	5	43	contain	contained	951:959	arg2	ACPCs					999:1003	ACPCs	999:1003	ACPCs	999:1003	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	43	contain	contained	951:959	arg2	Cells					992:996	Articular Cartilage Progenitor Cells	961:996	Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs)	961:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	43	contain	contained	951:959	arg1	layer					945:949	The top layer	937:949	The top layer	937:949	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	3	44	theme	equine	629:634	arg1	model					636:640	a long-term equine model	617:640	a long-term equine model	617:640	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	5	45	theme	top	941:943	arg1	layer					945:949	The top layer	937:949	The top layer	937:949	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	1	46	theme	personalized	229:240	arg1	constructs					256:265	personalized osteochondral constructs	229:265	personalized osteochondral constructs for joint repair	229:282	Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair.					
32434160	2	47	theme	Native	285:290	arg1	cartilage					302:310	Native articular cartilage	285:310	Native articular cartilage	285:310	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	1	48	theme	Recent	167:172	arg1	research					174:181	Recent research	167:181	Recent research	167:181	Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair.					
32434160	0	49	theme	in	118:119	arg1	performance					126:136	in vivo performance	118:136	in vivo performance in a long-term equine model	118:164	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	0	50	dep	thermoplast	32:42	arg1	performance					126:136	in vivo performance	118:136	in vivo performance in a long-term equine model	118:164	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	10	51	theme	implanted	1699:1707	arg1	cells					1709:1713	implanted cells	1699:1713	implanted cells	1699:1713	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	52	theme	cells	1709:1713	arg1	loss					1691:1694	early loss	1685:1694	early loss of implanted cells	1685:1713	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	52	theme	cells	1709:1713	arg1	rate					1745:1748	inappropriate degradation rate	1719:1748	inappropriate degradation rate of the hydrogel	1719:1764	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	3	53	theme	cellular	569:576	arg1	component					578:586	cellular component	569:586	cellular component	569:586	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	8	54	theme	hydrogel	1398:1405	arg1	persistency					1383:1393	persistency	1383:1393	persistency of hydrogel	1383:1405	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	6	55	theme	control	1151:1157	arg1	constructs					1159:1168	control constructs	1151:1168	control constructs	1151:1168	The chondral part of control constructs were homogeneously filled with MSCs.					
32434160	11	56	theme	construct	1870:1878	arg1	characteristics					1880:1894	construct characteristics	1870:1894	construct characteristics	1870:1894	Nonetheless, this approach may be promising and research into further tailoring of biomaterials and of construct characteristics seems warranted.					
32434160	4	57	theme	thiol-ene	828:836	arg1	HA-SH/P					901:907	HA-SH/P	901:907	HA-SH/P(AGE-co-G)	901:917	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	57	theme	thiol-ene	828:836	arg1	hydrogel					891:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel	826:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	826:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	58	theme	3D-printed	671:680	arg1	anchor					714:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor	669:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	669:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	0	59	theme	equine	153:158	arg1	model					160:164	a long-term equine model	141:164	a long-term equine model	141:164	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	4	60	theme	hyaluronic	853:862	arg1	HA-SH/P					901:907	HA-SH/P	901:907	HA-SH/P(AGE-co-G)	901:917	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	60	theme	hyaluronic	853:862	arg1	hydrogel					891:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel	826:898	a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	826:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	8	61	theme	zonal	1416:1420	arg1	constructs					1436:1445	zonal and non-zonal constructs	1416:1445	zonal and non-zonal constructs	1416:1445	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	8	62	theme	non-zonal	1426:1434	arg1	constructs					1436:1445	zonal and non-zonal constructs	1416:1445	zonal and non-zonal constructs	1416:1445	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	0	63	theme	zonal	82:86	arg1	approach					88:95	a zonal approach	80:95	a zonal approach to cartilage repair	80:115	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	5	64	theme	tissue	1061:1066	arg1	layer					1035:1039	the superficial layer	1019:1039	the superficial layer of native cartilage tissue	1019:1066	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	64	theme	tissue	1061:1066	arg1	layer					1080:1084	the bottom layer	1069:1084	the bottom layer contained mesenchymal stromal cells (MSCs)	1069:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	0	65	dep	in	118:119	arg1	vivo					121:124	vivo	121:124	vivo	121:124	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	7	66	theme	significant	1258:1268	arg1	growth					1275:1280	significant bone growth	1258:1280	significant bone growth	1258:1280	After six months in vivo, microtomography revealed significant bone growth into the anchor.					
32434160	4	67	theme	hydrogel	891:898	arg1	layers					816:821	two layers	812:821	two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G))	812:918	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	4	68	theme	bone	709:712	arg1	anchor					714:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor	669:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	669:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	2	69	theme	zonal	318:322	arg1	structure					324:332	a zonal structure	316:332	a zonal structure	316:332	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	5	70	attach	derived	1006:1012	arg2	ACPCs					999:1003	ACPCs	999:1003	ACPCs	999:1003	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	70	attach	derived	1006:1012	arg1	layer					1035:1039	the superficial layer	1019:1039	the superficial layer of native cartilage tissue	1019:1066	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	70	attach	derived	1006:1012	arg2	Cells					992:996	Articular Cartilage Progenitor Cells	961:996	Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs)	961:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	70	attach	derived	1006:1012	arg1	layer					1080:1084	the bottom layer	1069:1084	the bottom layer contained mesenchymal stromal cells (MSCs)	1069:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	0	71	theme	composite	2:10	arg1	hydrogel-3D					12:22	A composite hydrogel-3D	0:22	A composite hydrogel-3D printed	0:30	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	5	72	theme	native	1044:1049	arg1	tissue					1061:1066	native cartilage tissue	1044:1066	native cartilage tissue	1044:1066	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	2	73	theme	frequent	412:419	arg1	failure					421:427	the frequent failure	408:427	the frequent failure of these repair attempts	408:452	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	2	74	theme	current	361:367	arg1	constructs					369:378	current constructs	361:378	current constructs	361:378	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	4	75	theme	PCL	704:706	arg1	anchor					714:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor	669:719	a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated	669:934	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	0	76	theme	osteochondral	44:56	arg1	anchor					58:63	osteochondral anchor	44:63	osteochondral anchor	44:63	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	2	77	theme	repair	438:443	arg1	attempts					445:452	these repair attempts	432:452	these repair attempts	432:452	Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts.					
32434160	10	78	theme	sub-optimal	1599:1609	arg1	quality					1611:1617	The sub-optimal quality	1595:1617	The sub-optimal quality of the repair tissue	1595:1638	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	78	theme	sub-optimal	1599:1609	arg1	related					1647:1653	related	1647:1653	related	1647:1653	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	5	79	theme	Articular	961:969	arg1	ACPCs					999:1003	ACPCs	999:1003	ACPCs	999:1003	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	79	theme	Articular	961:969	arg1	Cells					992:996	Articular Cartilage Progenitor Cells	961:996	Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs)	961:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	8	80	theme	limited	1330:1336	arg1	production					1338:1347	only limited production	1325:1347	only limited production of cartilage-like tissue	1325:1372	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	5	81	theme	mesenchymal	1096:1106	arg1	cells					1116:1120	mesenchymal stromal cells	1096:1120	mesenchymal stromal cells (MSCs)	1096:1127	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	5	81	theme	mesenchymal	1096:1106	arg1	MSCs					1123:1126	MSCs	1123:1126	MSCs	1123:1126	The top layer contained Articular Cartilage Progenitor Cells (ACPCs) derived from the superficial layer of native cartilage tissue, the bottom layer contained mesenchymal stromal cells (MSCs).					
32434160	3	82	theme	component	578:586	arg1	distribution					553:564	the zonal distribution	543:564	the zonal distribution of cellular component	543:586	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	0	83	from	performance	126:136	arg1	model					160:164	a long-term equine model	141:164	a long-term equine model	141:164	A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.					
32434160	3	84	theme	long-term	619:627	arg1	model					636:640	a long-term equine model	617:640	a long-term equine model	617:640	Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model.					
32434160	4	85	theme	reinforcing	732:742	arg1	fibers					744:749	reinforcing fibers	732:749	reinforcing fibers	732:749	Constructs constituted of a 3D-printed poly(ϵ-caprolactone) (PCL) bone anchor from which reinforcing fibers protruded into the chondral part of the construct over which two layers of a thiol-ene cross-linkable hyaluronic acid/poly(glycidol) hybrid hydrogel (HA-SH/P(AGE-co-G)) were fabricated.					
32434160	10	86	theme	repair	1626:1631	arg1	tissue					1633:1638	the repair tissue	1622:1638	the repair tissue	1622:1638	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	8	87	theme	tissue	1367:1372	arg1	production					1338:1347	only limited production	1325:1347	only limited production of cartilage-like tissue	1325:1372	Histologically, there was only limited production of cartilage-like tissue (despite persistency of hydrogel) both in zonal and non-zonal constructs.					
32434160	10	88	theme	several	1658:1664	arg1	loss					1691:1694	early loss	1685:1694	early loss of implanted cells	1685:1713	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	88	theme	several	1658:1664	arg1	rate					1745:1748	inappropriate degradation rate	1719:1748	inappropriate degradation rate of the hydrogel	1719:1764	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
32434160	10	88	theme	several	1658:1664	arg1	factors					1666:1672	several factors	1658:1672	several factors	1658:1672	The sub-optimal quality of the repair tissue may be related to several factors, including early loss of implanted cells, or inappropriate degradation rate of the hydrogel.					
34303743	6	0	theme	kiwifruit	962:970	arg1	ripeness					972:979	kiwifruit ripeness	962:979	kiwifruit ripeness	962:979	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	7	1	theme	intelligent	987:997	arg1	paper					999:1003	This intelligent paper	982:1003	This intelligent paper	982:1003	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
34303743	4	2	theme	color	729:733	arg1	change					735:740	a color change	727:740	a color change in the indicators	727:758	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	0	3	theme	kiwifruit	117:125	arg1	maturity					127:134	kiwifruit maturity	117:134	kiwifruit maturity	117:134	Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity.					
34303743	6	4	used	used	914:917	arg2	application					890:900	A mobile phone application	875:900	A mobile phone application	875:900	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	4	5	theme	addition	620:627	arg1	reaction					629:636	The nucleophilic addition reaction	603:636	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction)	603:684	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	5	6	dep	red	870:872	arg1	to					867:868	to	867:868	to	867:868	As the kiwifruit ripens, the colorimetric paper changes from bluish-purple to dark red and then gradually to red.					
34303743	3	7	theme	respiration	572:582	arg1	effects					555:561	the effects	551:561	the effects of fruit respiration and transpiration	551:600	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	5	8	theme	kiwifruit	768:776	arg1	ripens					778:783	the kiwifruit ripens	764:783	the kiwifruit ripens	764:783	As the kiwifruit ripens, the colorimetric paper changes from bluish-purple to dark red and then gradually to red.					
34303743	0	9	theme	cellulose/chitosan/beeswax	55:80	arg1	papers					95:100	aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers	36:100	aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers	36:100	Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity.					
34303743	3	10	theme	fruit	566:570	arg1	respiration					572:582	fruit respiration	566:582	fruit respiration	566:582	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	5	11	theme	colorimetric	790:801	arg1	paper					803:807	the colorimetric paper	786:807	the colorimetric paper	786:807	As the kiwifruit ripens, the colorimetric paper changes from bluish-purple to dark red and then gradually to red.					
34303743	4	12	from	pH	698:699	arg1	paper					708:712	the paper	704:712	the paper	704:712	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	3	13	theme	color	457:461	arg1	indicators					463:472	color indicators	457:472	color indicators	457:472	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	13	theme	color	457:461	arg1	red					427:429	red	427:429	red	427:429	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	13	theme	color	457:461	arg1	violet					447:452	methyl red and bromocresol violet	420:452	violet	447:452	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	14	theme	cellulose	393:401	arg1	paper					403:407	cellulose paper	393:407	cellulose paper containing methyl red and bromocresol violet as color indicators	393:472	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	15	contain	containing	409:418	arg2	violet					447:452	methyl red and bromocresol violet	420:452	violet	447:452	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	15	contain	containing	409:418	arg2	indicators					463:472	color indicators	457:472	color indicators	457:472	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	15	contain	containing	409:418	arg1	paper					403:407	cellulose paper	393:407	cellulose paper containing methyl red and bromocresol violet as color indicators	393:472	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	15	contain	containing	409:418	arg2	red					427:429	red	427:429	red	427:429	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	16	theme	transpiration	588:600	arg1	effects					555:561	the effects	551:561	the effects of fruit respiration and transpiration	551:600	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	1	17	theme	kiwifruit	225:233	arg1	ripeness					213:220	the ripeness	209:220	the ripeness of kiwifruit	209:233	In this study, we developed an in-package colorimetric paper to monitor the ripeness of kiwifruit by detecting the release of aldehydes.					
34303743	3	18	theme	paper	403:407	arg1	piece					384:388	A piece	382:388	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators	382:472	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	4	19	theme	nucleophilic	607:618	arg1	reaction					629:636	The nucleophilic addition reaction	603:636	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction)	603:684	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	7	20	theme	real	1074:1077	arg1	time					1079:1082	real time	1074:1082	real time	1074:1082	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
34303743	6	21	theme	phone	884:888	arg1	application					890:900	A mobile phone application	875:900	A mobile phone application	875:900	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	3	22	theme	hydrophobic	502:512	arg1	films					514:518	two hydrophobic films	498:518	two hydrophobic films	498:518	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	23	theme	bromocresol	435:445	arg1	indicators					463:472	color indicators	457:472	color indicators	457:472	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	23	theme	bromocresol	435:445	arg1	red					427:429	red	427:429	red	427:429	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	3	23	theme	bromocresol	435:445	arg1	violet					447:452	methyl red and bromocresol violet	420:452	violet	447:452	A piece of cellulose paper containing methyl red and bromocresol violet as color indicators was heat-sealed between two hydrophobic films to protect the indicators from the effects of fruit respiration and transpiration.					
34303743	6	24	theme	mobile	877:882	arg1	application					890:900	A mobile phone application	875:900	A mobile phone application	875:900	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	6	25	attach	link	949:952	arg2	application					890:900	A mobile phone application	875:900	A mobile phone application	875:900	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	6	25	attach	link	949:952	arg1	ripeness					972:979	kiwifruit ripeness	962:979	kiwifruit ripeness	962:979	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	0	26	theme	aldehyde-sensitive	36:53	arg1	papers					95:100	aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers	36:100	aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers	36:100	Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity.					
34303743	7	27	theme	produce	1063:1069	arg1	monitoring					1049:1058	the accurate and convenient monitoring	1021:1058	the accurate and convenient monitoring of produce in real time	1021:1082	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
34303743	4	28	theme	Cannizzaro	665:674	arg1	OH-					660:662	OH-	660:662	OH-	660:662	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	4	28	theme	Cannizzaro	665:674	arg1	reaction					676:683	Cannizzaro reaction	665:683	Cannizzaro reaction	665:683	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	0	29	theme	papers	95:100	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity.					
34303743	0	29	theme	papers	95:100	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity.					
34303743	7	30	theme	convenient	1038:1047	arg1	monitoring					1049:1058	the accurate and convenient monitoring	1021:1058	the accurate and convenient monitoring of produce in real time	1021:1082	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
34303743	0	31	theme	colorimetric	82:93	arg1	papers					95:100	aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers	36:100	aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers	36:100	Development and characterization of aldehyde-sensitive cellulose/chitosan/beeswax colorimetric papers for monitoring kiwifruit maturity.					
34303743	7	32	used	used	1012:1015	arg2	paper					999:1003	This intelligent paper	982:1003	This intelligent paper	982:1003	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
34303743	7	33	theme	accurate	1025:1032	arg1	monitoring					1049:1058	the accurate and convenient monitoring	1021:1058	the accurate and convenient monitoring of produce in real time	1021:1082	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
34303743	2	34	theme	composite	295:303	arg1	films					305:309	Strongly hydrophobic composite films	274:309	Strongly hydrophobic composite films	274:309	Strongly hydrophobic composite films were prepared using chitosan as the matrix and beeswax as an additive.					
34303743	2	35	dep	matrix	347:352	arg1	the					343:345	the	343:345	the	343:345	Strongly hydrophobic composite films were prepared using chitosan as the matrix and beeswax as an additive.					
34303743	2	36	theme	hydrophobic	283:293	arg1	films					305:309	Strongly hydrophobic composite films	274:309	Strongly hydrophobic composite films	274:309	Strongly hydrophobic composite films were prepared using chitosan as the matrix and beeswax as an additive.					
34303743	1	37	theme	in-package	168:177	arg1	paper					192:196	an in-package colorimetric paper	165:196	an in-package colorimetric paper to monitor the ripeness of kiwifruit by detecting the release of aldehydes	165:271	In this study, we developed an in-package colorimetric paper to monitor the ripeness of kiwifruit by detecting the release of aldehydes.					
34303743	4	38	from	change	735:740	arg1	indicators					749:758	the indicators	745:758	the indicators	745:758	The nucleophilic addition reaction between aldehydes and OH- (Cannizzaro reaction) changes the pH in the paper and triggers a color change in the indicators.					
34303743	1	39	theme	aldehydes	263:271	arg1	release					252:258	the release	248:258	the release of aldehydes	248:271	In this study, we developed an in-package colorimetric paper to monitor the ripeness of kiwifruit by detecting the release of aldehydes.					
34303743	6	40	theme	RGB	934:936	arg1	values					938:943	the RGB values	930:943	the RGB values	930:943	A mobile phone application was further used to measure the RGB values and link them to kiwifruit ripeness.					
34303743	1	41	theme	colorimetric	179:190	arg1	paper					192:196	an in-package colorimetric paper	165:196	an in-package colorimetric paper to monitor the ripeness of kiwifruit by detecting the release of aldehydes	165:271	In this study, we developed an in-package colorimetric paper to monitor the ripeness of kiwifruit by detecting the release of aldehydes.					
34303743	7	42	from	monitoring	1049:1058	arg1	time					1079:1082	real time	1074:1082	real time	1074:1082	This intelligent paper can be used for the accurate and convenient monitoring of produce in real time.					
33955492	10	0	theme	SSP-related	1406:1416	arg1	genes					1418:1422	starch- and SSP-related genes	1394:1422	starch- and SSP-related genes	1394:1422	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	3	1	from	levels	420:425	arg1	seeds					430:434	seeds	430:434	seeds	430:434	Moreover, starch levels in seeds are associated with yield.					
33955492	2	2	dep	content	277:283	arg1	the					273:275	the	273:275	the	273:275	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	7	3	theme	knock-out	842:850	arg1	mutants					852:858	Triple knock-out mutants	835:858	Triple knock-out mutants of all three TaNAC019 homoeologs	835:891	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	10	4	theme	grain	1447:1451	arg1	quality					1453:1459	wheat grain quality	1441:1459	wheat grain quality	1441:1459	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	8	5	theme	HMW-GS	1123:1128	arg1	genes					1130:1134	HMW-GS genes	1123:1134	HMW-GS genes	1123:1134	TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb.					
33955492	7	6	theme	quality	1057:1063	arg1	parameters					1065:1074	flour processing quality parameters	1040:1074	flour processing quality parameters	1040:1074	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	10	7	theme	TaNAC019	1371:1378	arg1	regulation					1380:1389	TaNAC019 regulation	1371:1389	TaNAC019 regulation of starch- and SSP-related genes	1371:1422	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	5	8	theme	endosperm-specific	606:623	arg1	factor					643:648	the endosperm-specific NAC transcription factor	602:648	the endosperm-specific NAC transcription factor TaNAC019	602:657	In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation.					
33955492	7	9	theme	transcript	911:920	arg1	levels					922:927	reduced transcript levels	903:927	reduced transcript levels for all SSP types	903:945	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	2	10	theme	seed	304:307	arg1	SSPs					327:330	SSPs	327:330	SSPs	327:330	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	2	10	theme	seed	304:307	arg1	proteins					317:324	seed storage proteins	304:324	seed storage proteins (SSPs)	304:331	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	0	11	theme	elite	104:108	arg1	allele					110:115	its elite allele	100:115	its elite allele	100:115	The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.					
33955492	5	12	theme	NAC	625:627	arg1	factor					643:648	the endosperm-specific NAC transcription factor	602:648	the endosperm-specific NAC transcription factor TaNAC019	602:657	In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation.					
33955492	6	13	theme	molecular	767:775	arg1	weight					777:782	high molecular weight	762:782	high molecular weight glutenin (HMW-GS)	762:800	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	0	14	theme	wheat	126:130	arg1	quality					138:144	wheat grain quality	126:144	wheat grain quality	126:144	The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.					
33955492	10	15	theme	starch-	1394:1400	arg1	genes					1418:1422	starch- and SSP-related genes	1394:1422	starch- and SSP-related genes	1394:1422	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	8	16	theme	genes	1130:1134	arg1	expression					1109:1118	the expression	1105:1118	the expression of HMW-GS genes	1105:1134	TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb.					
33955492	6	17	theme	high	762:765	arg1	weight					777:782	high molecular weight	762:782	high molecular weight glutenin (HMW-GS)	762:800	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	3	18	theme	starch	413:418	arg1	levels					420:425	starch levels	413:425	starch levels in seeds	413:434	Moreover, starch levels in seeds are associated with yield.					
33955492	4	19	from	accumulation	540:551	arg1	wheat					556:560	wheat	556:560	wheat	556:560	However, little is known about the mechanisms that coordinate SSP and starch accumulation in wheat.					
33955492	8	20	from	motif	1161:1165	arg1	promoters					1176:1184	their promoters	1170:1184	their promoters	1170:1184	TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb.					
33955492	7	21	theme	flour	1040:1044	arg1	parameters					1065:1074	flour processing quality parameters	1040:1074	flour processing quality parameters	1040:1074	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	2	22	theme	dough	367:371	arg1	elasticity					347:356	the elasticity	343:356	the elasticity of wheat dough and flour processing quality	343:400	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	0	23	theme	endosperm-specific	4:21	arg1	factor					37:42	The endosperm-specific transcription factor TaNAC019	0:51	The endosperm-specific transcription factor TaNAC019	0:51	The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.					
33955492	6	24	theme	genes	828:832	arg1	promoters					726:734	the promoters	722:734	the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes	722:832	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	7	25	theme	reduced	903:909	arg1	levels					922:927	reduced transcript levels	903:927	reduced transcript levels for all SSP types	903:945	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	1	26	dep	Triticum	157:164	arg1	L.					175:176	Triticum aestivum L.	157:176	Triticum aestivum L.	157:176	In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality.					
33955492	2	27	theme	wheat	361:365	arg1	dough					367:371	wheat dough	361:371	wheat dough	361:371	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	10	28	theme	wheat	1441:1445	arg1	quality					1453:1459	wheat grain quality	1441:1459	wheat grain quality	1441:1459	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	9	29	theme	TaSPA	1291:1295	arg1	expression					1277:1286	the expression	1273:1286	the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation	1273:1357	TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation.					
33955492	10	30	theme	genes	1418:1422	arg1	regulation					1380:1389	TaNAC019 regulation	1371:1389	TaNAC019 regulation of starch- and SSP-related genes	1371:1422	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	6	31	theme	loci	747:750	arg1	promoters					726:734	the promoters	722:734	the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes	722:832	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	1	32	theme	breeding	180:187	arg1	efforts					189:195	breeding efforts	180:195	breeding efforts	180:195	In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality.					
33955492	4	33	from	SSP	525:527	arg1	wheat					556:560	wheat	556:560	wheat	556:560	However, little is known about the mechanisms that coordinate SSP and starch accumulation in wheat.					
33955492	7	34	theme	TaNAC019	873:880	arg1	homoeologs					882:891	all three TaNAC019 homoeologs	863:891	all three TaNAC019 homoeologs	863:891	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	7	35	theme	starch	1016:1021	arg1	contents					1023:1030	lower gluten and starch contents	999:1030	lower gluten and starch contents	999:1030	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	6	36	theme	TaGlu-1	739:745	arg1	loci					747:750	TaGlu-1 loci	739:750	TaGlu-1 loci	739:750	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	11	37	theme	elite	1488:1492	arg1	TaNAC019-BI					1502:1512	TaNAC019-BI	1502:1512	TaNAC019-BI	1502:1512	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	11	37	theme	elite	1488:1492	arg1	allele					1494:1499	an elite allele	1485:1499	an elite allele (TaNAC019-BI) associated with flour processing quality	1485:1554	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	8	38	theme	TaGlu-1	1211:1217	arg1	TaGAMyb					1229:1235	the TaGlu-1 regulator TaGAMyb	1207:1235	the TaGlu-1 regulator TaGAMyb	1207:1235	TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb.					
33955492	0	39	theme	transcription	23:35	arg1	factor					37:42	The endosperm-specific transcription factor TaNAC019	0:51	The endosperm-specific transcription factor TaNAC019	0:51	The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.					
33955492	4	40	theme	starch	533:538	arg1	accumulation					540:551	starch accumulation	533:551	starch accumulation	533:551	However, little is known about the mechanisms that coordinate SSP and starch accumulation in wheat.					
33955492	0	41	theme	grain	132:136	arg1	quality					138:144	wheat grain quality	126:144	wheat grain quality	126:144	The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.					
33955492	6	42	theme	metabolism	817:826	arg1	genes					828:832	starch metabolism genes	810:832	starch metabolism genes	810:832	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	10	43	contain	has	1424:1426	arg2	roles					1432:1436	key roles	1428:1436	key roles	1428:1436	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	10	43	contain	has	1424:1426	arg1	regulation					1380:1389	TaNAC019 regulation	1371:1389	TaNAC019 regulation of starch- and SSP-related genes	1371:1422	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	7	44	theme	gluten	1005:1010	arg1	contents					1023:1030	lower gluten and starch contents	999:1030	lower gluten and starch contents	999:1030	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	6	45	theme	starch	810:815	arg1	genes					828:832	starch metabolism genes	810:832	starch metabolism genes	810:832	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	6	46	dep	weight	777:782	arg1	HMW-GS					794:799	HMW-GS	794:799	HMW-GS	794:799	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	6	46	dep	weight	777:782	arg1	glutenin					784:791	glutenin	784:791	high molecular weight glutenin (HMW-GS)	762:800	TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes.					
33955492	2	47	theme	processing	383:392	arg1	elasticity					347:356	the elasticity	343:356	the elasticity of wheat dough and flour processing quality	343:400	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	8	48	theme	specific	1152:1159	arg1	motif					1161:1165	a specific motif	1150:1165	a specific motif in their promoters	1150:1184	TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb.					
33955492	5	49	theme	starch	683:688	arg1	accumulation					690:701	starch accumulation	683:701	starch accumulation	683:701	In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation.					
33955492	7	50	theme	starch	969:974	arg1	metabolism					976:985	starch metabolism	969:985	starch metabolism	969:985	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	2	51	theme	flour	377:381	arg1	processing					383:392	flour processing	377:392	flour processing	377:392	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	9	52	theme	maize	1313:1317	arg1	Opaque2					1319:1325	maize Opaque2	1313:1325	maize Opaque2	1313:1325	TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation.					
33955492	5	53	theme	transcription	629:641	arg1	factor					643:648	the endosperm-specific NAC transcription factor	602:648	the endosperm-specific NAC transcription factor TaNAC019	602:657	In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation.					
33955492	11	54	theme	improved	1608:1615	arg1	quality					1617:1623	improved quality	1608:1623	improved quality	1608:1623	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	11	55	with	wheat	1597:1601	arg1	quality					1617:1623	improved quality	1608:1623	improved quality	1608:1623	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	9	56	theme	Opaque2	1319:1325	arg1	TaSPA					1291:1295	TaSPA	1291:1295	TaSPA	1291:1295	TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation.					
33955492	9	56	theme	Opaque2	1319:1325	arg1	ortholog					1301:1308	an ortholog	1298:1308	an ortholog of maize Opaque2 that activates SSP accumulation	1298:1357	TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation.					
33955492	5	57	theme	factor	643:648	arg1	TaNAC019					650:657	the endosperm-specific NAC transcription factor TaNAC019	602:657	the endosperm-specific NAC transcription factor TaNAC019	602:657	In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation.					
33955492	10	58	theme	key	1428:1430	arg1	roles					1432:1436	key roles	1428:1436	key roles	1428:1436	Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality.					
33955492	7	59	theme	processing	1046:1055	arg1	parameters					1065:1074	flour processing quality parameters	1040:1074	flour processing quality parameters	1040:1074	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	7	60	theme	homoeologs	882:891	arg1	mutants					852:858	Triple knock-out mutants	835:858	Triple knock-out mutants of all three TaNAC019 homoeologs	835:891	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	5	61	theme	TaNAC019	650:657	arg1	role					594:597	the role	590:597	the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation	590:701	In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation.					
33955492	11	62	theme	flour	1531:1535	arg1	quality					1548:1554	flour processing quality	1531:1554	flour processing quality	1531:1554	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	2	63	theme	proteins	317:324	arg1	composition					289:299	composition	289:299	composition	289:299	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	2	63	theme	proteins	317:324	arg1	content					277:283	content	277:283	content	277:283	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	11	64	theme	processing	1537:1546	arg1	quality					1548:1554	flour processing quality	1531:1554	flour processing quality	1531:1554	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	2	65	theme	storage	309:315	arg1	SSPs					327:330	SSPs	327:330	SSPs	327:330	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	2	65	theme	storage	309:315	arg1	proteins					317:324	seed storage proteins	304:324	seed storage proteins (SSPs)	304:331	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	9	66	theme	SSP	1342:1344	arg1	accumulation					1346:1357	SSP accumulation	1342:1357	SSP accumulation	1342:1357	TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation.					
33955492	8	67	theme	regulator	1219:1227	arg1	TaGAMyb					1229:1235	the TaGlu-1 regulator TaGAMyb	1207:1235	the TaGlu-1 regulator TaGAMyb	1207:1235	TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb.					
33955492	11	68	theme	candidate	1569:1577	arg1	gene					1579:1582	a candidate gene	1567:1582	a candidate gene for breeding wheat with improved quality	1567:1623	Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.					
33955492	1	69	theme	grain	235:239	arg1	yield					241:245	grain yield	235:245	grain yield	235:245	In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality.					
33955492	0	70	theme	starch	76:81	arg1	accumulation					83:94	starch accumulation	76:94	starch accumulation	76:94	The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.					
33955492	2	71	dep	dough	367:371	arg1	quality					394:400	quality	394:400	quality	394:400	For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality.					
33955492	7	72	theme	SSP	937:939	arg1	types					941:945	all SSP types	933:945	all SSP types	933:945	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
33955492	7	73	theme	Triple	835:840	arg1	mutants					852:858	Triple knock-out mutants	835:858	Triple knock-out mutants of all three TaNAC019 homoeologs	835:891	Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters.					
32450354	8	0	theme	fixation	1109:1116	arg1	performance					1118:1128	superior phosphate fixation performance	1090:1128	superior phosphate fixation performance	1090:1128	Moreover, TAL-7 beads exhibited superior phosphate fixation performance in the long-term experiment.					
32450354	5	1	theme	TAL-7	600:604	arg1	beads					606:610	The optimized TAL-7 beads	586:610	The optimized TAL-7 beads	586:610	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	6	2	theme	maximum	828:834	arg1	uptake					836:841	a maximum uptake	826:841	a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4	826:885	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32450354	9	3	theme	phosphate	1313:1321	arg1	adsorption					1323:1332	phosphate adsorption	1313:1332	phosphate adsorption	1313:1332	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	1	4	theme	lanthanum	224:232	arg1	ion					234:236	lanthanum ion	224:236	lanthanum ion	224:236	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	9	5	theme	promising	1288:1296	arg1	biosorbent					1298:1307	a cost-effective and promising biosorbent	1267:1307	a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment	1267:1372	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	9	5	theme	promising	1288:1296	arg1	beads					1252:1256	TAL-7 beads	1246:1256	TAL-7 beads	1246:1256	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	6	6	theme	phosphate	777:785	arg1	efficiency					795:804	The phosphate removal efficiency	773:804	The phosphate removal efficiency	773:804	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32450354	2	7	theme	composites	333:342	arg1	characterization					313:328	the characterization	309:328	the characterization of composites	309:342	Multiple methods were applied for the characterization of composites.					
32450354	5	8	theme	anions	697:702	arg1	coexistence					672:682	the coexistence	668:682	the coexistence of competing anions	668:702	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	4	9	theme	adsorbent	490:498	arg1	content					461:467	talc content	456:467	talc content	456:467	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	9	theme	adsorbent	490:498	arg1	dosage					500:505	adsorbent dosage	490:505	adsorbent dosage	490:505	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	7	10	theme	Langmuir	923:930	arg1	model					941:945	Langmuir isotherm model	923:945	Langmuir isotherm model	923:945	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	2	11	theme	Multiple	275:282	arg1	methods					284:290	Multiple methods	275:290	Multiple methods	275:290	Multiple methods were applied for the characterization of composites.					
32450354	3	12	theme	lanthanum	377:385	arg1	effect					358:363	The combined effect	345:363	The combined effect of talc and lanthanum	345:385	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	3	13	theme	combined	349:356	arg1	effect					358:363	The combined effect	345:363	The combined effect of talc and lanthanum	345:385	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	6	14	theme	16.4 mg P/g	846:856	arg1	uptake					836:841	a maximum uptake	826:841	a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4	826:885	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32450354	5	15	theme	satisfactory	622:633	arg1	selectivity					635:645	satisfactory selectivity	622:645	satisfactory selectivity towards phosphate	622:663	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	8	16	theme	phosphate	1099:1107	arg1	performance					1118:1128	superior phosphate fixation performance	1090:1128	superior phosphate fixation performance	1090:1128	Moreover, TAL-7 beads exhibited superior phosphate fixation performance in the long-term experiment.					
32450354	9	17	from	biosorbent	1298:1307	arg1	environment					1362:1372	the aqueous environment	1350:1372	the aqueous environment	1350:1372	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	4	18	theme	talc	456:459	arg1	concentration					475:487	La3+ concentration	470:487	La3+ concentration	470:487	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	18	theme	talc	456:459	arg1	pH					508:509	pH	508:509	pH	508:509	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	18	theme	talc	456:459	arg1	content					461:467	talc content	456:467	talc content	456:467	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	18	theme	talc	456:459	arg1	ions					524:527	co-existing ions	512:527	co-existing ions (Cl-, NO3- and SO42-)	512:549	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	18	theme	talc	456:459	arg1	dosage					500:505	adsorbent dosage	490:505	adsorbent dosage	490:505	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	5	19	theme	4-6	768:770	arg1	range					759:763	the pH range	752:763	the pH range of 4-6	752:770	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	1	20	theme	encapsulated	145:156	arg1	beads					179:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	4	21	theme	La3+	470:473	arg1	content					461:467	talc content	456:467	talc content	456:467	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	21	theme	La3+	470:473	arg1	concentration					475:487	La3+ concentration	470:487	La3+ concentration	470:487	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	9	22	theme	cost-effective	1269:1282	arg1	biosorbent					1298:1307	a cost-effective and promising biosorbent	1267:1307	a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment	1267:1372	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	9	22	theme	cost-effective	1269:1282	arg1	beads					1252:1256	TAL-7 beads	1246:1256	TAL-7 beads	1246:1256	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	9	23	theme	experiment	1187:1196	arg1	analysis					1219:1226	adsorption experiment and characterization analysis	1176:1226	adsorption experiment and characterization analysis	1176:1226	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	1	24	theme	salts	158:162	arg1	beads					179:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	3	25	theme	talc	368:371	arg1	effect					358:363	The combined effect	345:363	The combined effect of talc and lanthanum	345:385	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	4	26	dep	ions	524:527	arg1	SO42-					544:548	SO42-	544:548	SO42-	544:548	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	26	dep	ions	524:527	arg1	NO3-					535:538	NO3-	535:538	NO3-	535:538	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	26	dep	ions	524:527	arg1	Cl-					530:532	Cl-	530:532	Cl-	530:532	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	26	dep	ions	524:527	arg1	ions					524:527	co-existing ions	512:527	co-existing ions (Cl-, NO3- and SO42-)	512:549	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	7	27	theme	beads	1051:1055	arg1	process					1034:1040	the phosphate adsorption process	1009:1040	the phosphate adsorption process of TAL-7 beads	1009:1055	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	7	28	theme	Further	888:894	arg1	experiments					896:906	Further experiments	888:906	Further experiments	888:906	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	7	29	theme	phosphate	1013:1021	arg1	process					1034:1040	the phosphate adsorption process	1009:1040	the phosphate adsorption process of TAL-7 beads	1009:1055	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	1	30	theme	alginate	164:171	arg1	beads					179:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	0	31	theme	Novel	0:4	arg1	talc					6:9	Novel talc	0:9	Novel talc	0:9	Novel talc encapsulated lanthanum alginate hydrogel for efficient phosphate adsorption and fixation.					
32450354	8	32	theme	TAL-7	1068:1072	arg1	beads					1074:1078	TAL-7 beads	1068:1078	TAL-7 beads	1068:1078	Moreover, TAL-7 beads exhibited superior phosphate fixation performance in the long-term experiment.					
32450354	5	33	theme	optimized	590:598	arg1	beads					606:610	The optimized TAL-7 beads	586:610	The optimized TAL-7 beads	586:610	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	9	34	theme	characterization	1202:1217	arg1	analysis					1219:1226	adsorption experiment and characterization analysis	1176:1226	adsorption experiment and characterization analysis	1176:1226	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	0	35	theme	lanthanum	24:32	arg1	hydrogel					43:50	lanthanum alginate hydrogel	24:50	lanthanum alginate hydrogel	24:50	Novel talc encapsulated lanthanum alginate hydrogel for efficient phosphate adsorption and fixation.					
32450354	1	36	theme	phosphate	253:261	arg1	adsorption					263:272	phosphate adsorption	253:272	phosphate adsorption	253:272	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	9	37	theme	adsorption	1176:1185	arg1	experiment					1187:1196	adsorption experiment	1176:1196	adsorption experiment	1176:1196	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	1	38	theme	TAL	174:176	arg1	beads					179:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	9	39	theme	aqueous	1354:1360	arg1	environment					1362:1372	the aqueous environment	1350:1372	the aqueous environment	1350:1372	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	7	40	theme	TAL-7	1045:1049	arg1	beads					1051:1055	TAL-7 beads	1045:1055	TAL-7 beads	1045:1055	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	1	41	theme	present	108:114	arg1	work					116:119	the present work	104:119	the present work	104:119	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	3	42	theme	phosphate	396:404	arg1	performance					414:424	phosphate removal performance	396:424	phosphate removal performance of TAL beads	396:437	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	7	43	theme	adsorption	1023:1032	arg1	process					1034:1040	the phosphate adsorption process	1009:1040	the phosphate adsorption process of TAL-7 beads	1009:1055	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	6	44	dep	pH	882:883	arg1	4					885:885	4	885:885	4	885:885	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32450354	3	45	theme	TAL	429:431	arg1	beads					433:437	TAL beads	429:437	TAL beads	429:437	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	0	46	theme	alginate	34:41	arg1	hydrogel					43:50	lanthanum alginate hydrogel	24:50	lanthanum alginate hydrogel	24:50	Novel talc encapsulated lanthanum alginate hydrogel for efficient phosphate adsorption and fixation.					
32450354	9	47	theme	TAL-7	1246:1250	arg1	biosorbent					1298:1307	a cost-effective and promising biosorbent	1267:1307	a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment	1267:1372	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	9	47	theme	TAL-7	1246:1250	arg1	beads					1252:1256	TAL-7 beads	1246:1256	TAL-7 beads	1246:1256	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	0	48	theme	efficient	56:64	arg1	adsorption					76:85	efficient phosphate adsorption	56:85	efficient phosphate adsorption	56:85	Novel talc encapsulated lanthanum alginate hydrogel for efficient phosphate adsorption and fixation.					
32450354	8	49	theme	long-term	1137:1145	arg1	experiment					1147:1156	the long-term experiment	1133:1156	the long-term experiment	1133:1156	Moreover, TAL-7 beads exhibited superior phosphate fixation performance in the long-term experiment.					
32450354	5	50	theme	phosphate	731:739	arg1	removal					741:747	efficient phosphate removal	721:747	efficient phosphate removal	721:747	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	5	51	theme	competing	687:695	arg1	anions					697:702	competing anions	687:702	competing anions	687:702	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	4	52	theme	co-existing	512:522	arg1	SO42-					544:548	SO42-	544:548	SO42-	544:548	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	52	theme	co-existing	512:522	arg1	NO3-					535:538	NO3-	535:538	NO3-	535:538	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	52	theme	co-existing	512:522	arg1	Cl-					530:532	Cl-	530:532	Cl-	530:532	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	52	theme	co-existing	512:522	arg1	content					461:467	talc content	456:467	talc content	456:467	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	4	52	theme	co-existing	512:522	arg1	ions					524:527	co-existing ions	512:527	co-existing ions (Cl-, NO3- and SO42-)	512:549	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	6	53	theme	optimal	874:880	arg1	pH					882:883	the optimal pH 4	870:885	the optimal pH 4	870:885	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32450354	7	54	theme	pseudo-second-order	955:973	arg1	model					983:987	the pseudo-second-order kinetic model	951:987	the pseudo-second-order kinetic model	951:987	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	9	55	from	analysis	1219:1226	arg1	results					1163:1169	The results	1159:1169	The results from adsorption experiment and characterization analysis	1159:1226	The results from adsorption experiment and characterization analysis demonstrated that TAL-7 beads could be a cost-effective and promising biosorbent for phosphate adsorption and fixation in the aqueous environment.					
32450354	8	56	theme	superior	1090:1097	arg1	performance					1118:1128	superior phosphate fixation performance	1090:1128	superior phosphate fixation performance	1090:1128	Moreover, TAL-7 beads exhibited superior phosphate fixation performance in the long-term experiment.					
32450354	7	57	theme	kinetic	975:981	arg1	model					983:987	the pseudo-second-order kinetic model	951:987	the pseudo-second-order kinetic model	951:987	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	1	58	theme	talc	122:125	arg1	beads					179:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	1	59	with	cross-linking	205:217	arg1	ion					234:236	lanthanum ion	224:236	lanthanum ion	224:236	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	5	60	theme	efficient	721:729	arg1	removal					741:747	efficient phosphate removal	721:747	efficient phosphate removal	721:747	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	6	61	theme	removal	787:793	arg1	efficiency					795:804	The phosphate removal efficiency	773:804	The phosphate removal efficiency	773:804	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32450354	3	62	theme	beads	433:437	arg1	performance					414:424	phosphate removal performance	396:424	phosphate removal performance of TAL beads	396:437	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	4	63	theme	batch	567:571	arg1	experiments					573:583	batch experiments	567:583	batch experiments	567:583	Factors such as talc content, La3+ concentration, adsorbent dosage, pH, co-existing ions (Cl-, NO3- and SO42-) were studied in batch experiments.					
32450354	0	64	theme	phosphate	66:74	arg1	adsorption					76:85	efficient phosphate adsorption	56:85	efficient phosphate adsorption	56:85	Novel talc encapsulated lanthanum alginate hydrogel for efficient phosphate adsorption and fixation.					
32450354	3	65	theme	removal	406:412	arg1	performance					414:424	phosphate removal performance	396:424	phosphate removal performance of TAL beads	396:437	The combined effect of talc and lanthanum improved phosphate removal performance of TAL beads.					
32450354	5	66	theme	pH	756:757	arg1	range					759:763	the pH range	752:763	the pH range of 4-6	752:770	The optimized TAL-7 beads exhibited satisfactory selectivity towards phosphate in the coexistence of competing anions and could remain efficient phosphate removal in the pH range of 4-6.					
32450354	7	67	theme	isotherm	932:939	arg1	model					941:945	Langmuir isotherm model	923:945	Langmuir isotherm model	923:945	Further experiments suggested that Langmuir isotherm model and the pseudo-second-order kinetic model could well describe the phosphate adsorption process of TAL-7 beads.					
32450354	1	68	theme	low-cost	130:137	arg1	talc					122:125	talc	122:125	talc (a low-cost clay) encapsulated salts alginate (TAL) beads	122:183	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	1	68	theme	low-cost	130:137	arg1	clay					139:142	a low-cost clay	128:142	a low-cost clay	128:142	In the present work, talc (a low-cost clay) encapsulated salts alginate (TAL) beads were synthesized by cross-linking with lanthanum ion and tested for phosphate adsorption.					
32450354	6	69	with	%	819:819	arg1	uptake					836:841	a maximum uptake	826:841	a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4	826:885	The phosphate removal efficiency reached to 95% with a maximum uptake of 16.4 mg P/g obtained at the optimal pH 4.					
32659617	9	0	theme	treatment	1488:1496	arg1	time					1498:1501	the treatment time	1484:1501	the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells	1484:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	1	1	theme	food	222:225	arg1	industry					227:234	the food industry	218:234	the food industry	218:234	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	6	2	theme	mild	1066:1069	arg1	52 °C					1077:1081	mild heat (52 °C)	1066:1082	mild heat (52 °C)	1066:1082	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	0	3	theme	fruit	72:76	arg1	juices					78:83	fruit juices	72:83	fruit juices	72:83	Chitosan nanoemulsions of cold-pressed orange essential oil to preserve fruit juices.					
32659617	3	4	theme	mild	528:531	arg1	heat					533:536	mild heat	528:536	mild heat	528:536	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	11	5	theme	mild	1893:1896	arg1	heat					1898:1901	mild heat	1893:1901	mild heat	1893:1901	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	6	6	theme	activity	1037:1044	arg1	terms					1014:1018	terms	1014:1018	terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	1014:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	9	7	theme	CSEO	1420:1423	arg1	combination					1405:1415	the combination	1401:1415	the combination of CSEO and mild heat	1401:1437	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	11	8	theme	advantageous	1849:1860	arg1	carrier					1742:1748	a promising carrier	1730:1748	a promising carrier for lipophilic substances	1730:1774	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	11	8	theme	advantageous	1849:1860	arg1	alternative					1862:1872	an advantageous alternative	1846:1872	an advantageous alternative	1846:1872	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	3	9	from	heat	533:536	arg1	orange					581:586	orange	581:586	orange	581:586	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	3	9	from	heat	533:536	arg1	juices					598:603	apple juices	592:603	apple juices	592:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	5	10	theme	shear	766:770	arg1	conditions					772:781	low shear conditions	762:781	low shear conditions	762:781	cn-CSEO was prepared under low shear conditions and characterized according to droplet size (<60 nm) and polydispersity index (<0.260 nm).					
32659617	11	11	theme	fruit	1915:1919	arg1	juices					1921:1926	fruit juices	1915:1926	fruit juices	1915:1926	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	8	12	theme	mild	1334:1337	arg1	heat					1339:1342	mild heat	1334:1342	mild heat in apple juice	1334:1357	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	10	13	theme	sensory	1598:1604	arg1	acceptable					1642:1651	acceptable	1642:1651	acceptable	1642:1651	Finally, the sensory characteristics of both juices were acceptable either when using s-CSEO or CSEO nanoemulsified with chitosan.					
32659617	10	13	theme	sensory	1598:1604	arg1	characteristics					1606:1620	the sensory characteristics	1594:1620	the sensory characteristics of both juices	1594:1635	Finally, the sensory characteristics of both juices were acceptable either when using s-CSEO or CSEO nanoemulsified with chitosan.					
32659617	11	14	theme	EOs	1798:1800	arg1	encapsulation					1781:1793	the encapsulation	1777:1793	the encapsulation of EOs with chitosan nanoemulsions	1777:1828	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	6	15	theme	Escherichia	1092:1102	arg1	O157					1109:1112	Escherichia coli O157	1092:1112	Escherichia coli O157:H7 Sakai	1092:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	6	16	dep	Escherichia	1092:1102	arg1	coli					1104:1107	coli	1104:1107	coli	1104:1107	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	5	17	theme	polydispersity	840:853	arg1	<0.260 nm					862:870	<0.260 nm	862:870	<0.260 nm	862:870	cn-CSEO was prepared under low shear conditions and characterized according to droplet size (<60 nm) and polydispersity index (<0.260 nm).					
32659617	5	17	theme	polydispersity	840:853	arg1	index					855:859	polydispersity index	840:859	polydispersity index (<0.260 nm)	840:871	cn-CSEO was prepared under low shear conditions and characterized according to droplet size (<60 nm) and polydispersity index (<0.260 nm).					
32659617	6	18	dep	O157	1109:1112	arg1	H7					1114:1115	H7	1114:1115	Escherichia coli O157:H7 Sakai	1092:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	9	19	dep	cycles	1546:1551	arg1	Log10					1540:1544	Log10	1540:1544	Log10	1540:1544	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	10	20	theme	juices	1630:1635	arg1	acceptable					1642:1651	acceptable	1642:1651	acceptable	1642:1651	Finally, the sensory characteristics of both juices were acceptable either when using s-CSEO or CSEO nanoemulsified with chitosan.					
32659617	10	20	theme	juices	1630:1635	arg1	characteristics					1606:1620	the sensory characteristics	1594:1620	the sensory characteristics of both juices	1594:1635	Finally, the sensory characteristics of both juices were acceptable either when using s-CSEO or CSEO nanoemulsified with chitosan.					
32659617	5	21	theme	droplet	814:820	arg1	size					822:825	droplet size	814:825	droplet size (<60 nm)	814:834	cn-CSEO was prepared under low shear conditions and characterized according to droplet size (<60 nm) and polydispersity index (<0.260 nm).					
32659617	5	21	theme	droplet	814:820	arg1	<60 nm					828:833	<60 nm	828:833	<60 nm	828:833	cn-CSEO was prepared under low shear conditions and characterized according to droplet size (<60 nm) and polydispersity index (<0.260 nm).					
32659617	9	22	theme	Sakai	1572:1576	arg1	cells					1578:1582	E. coli O157:H7 Sakai cells	1556:1582	E. coli O157:H7 Sakai cells	1556:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	9	23	dep	5	1538:1538	arg1	to					1535:1536	to	1535:1536	to	1535:1536	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	1	24	theme	sinensis	150:157	arg1	peels					134:138	the peels	130:138	the peels of Citrus sinensis (CSEO)	130:164	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	6	25	theme	least	907:911	arg1	3 months					913:920	at least 3 months	904:920	at least 3 months	904:920	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	6	26	dep	least	907:911	arg1	at					904:905	at	904:905	at	904:905	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	3	27	from	activity	499:506	arg1	combination					511:521	combination	511:521	combination with mild heat, as well as its sensorial acceptability in orange and apple juices	511:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	9	28	dep	O157	1564:1567	arg1	H7					1569:1570	H7	1569:1570	E. coli O157:H7 Sakai cells	1556:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	4	29	theme	predominant	702:712	arg1	constituent					714:724	the predominant constituent	698:724	the predominant constituent (95.1%)	698:732	CSEO composition was analyzed by GC-MS, and 19 components were identified, with limonene as the predominant constituent (95.1%).					
32659617	4	29	theme	predominant	702:712	arg1	%					731:731	95.1%	727:731	95.1%	727:731	CSEO composition was analyzed by GC-MS, and 19 components were identified, with limonene as the predominant constituent (95.1%).					
32659617	9	30	dep	E.	1556:1557	arg1	coli					1559:1562	coli	1559:1562	coli	1559:1562	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	9	31	dep	Log10	1540:1544	arg1	5					1538:1538	5	1538:1538	5	1538:1538	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	3	32	from	acceptability	564:576	arg1	orange					581:586	orange	581:586	orange	581:586	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	3	32	from	acceptability	564:576	arg1	juices					598:603	apple juices	592:603	apple juices	592:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	3	33	with	combination	511:521	arg1	heat					533:536	mild heat	528:536	mild heat	528:536	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	3	33	with	combination	511:521	arg1	acceptability					564:576	its sensorial acceptability	550:576	its sensorial acceptability in orange and apple juices	550:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	1	34	theme	orange	92:97	arg1	product					207:213	a valuable product	196:213	a valuable product by the food industry	196:234	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	1	34	theme	orange	92:97	arg1	oil					109:111	Sweet orange essential oil	86:111	Sweet orange essential oil	86:111	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	6	35	theme	CSEO	977:980	arg1	suspensions					962:972	suspensions	962:972	suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	962:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	8	36	theme	bactericidal	1258:1269	arg1	effect					1271:1276	an improved bactericidal effect	1246:1276	an improved bactericidal effect of cn-CSEO in comparison with s-CSEO	1246:1313	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	9	37	theme	mild	1429:1432	arg1	heat					1434:1437	mild heat	1429:1437	mild heat	1429:1437	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	3	38	theme	apple	592:596	arg1	juices					598:603	apple juices	592:603	apple juices	592:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	2	39	theme	products	365:372	arg1	delivery					344:351	delivery	344:351	delivery of EO-based products	344:372	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	2	39	theme	products	365:372	arg1	stability					304:312	chemical stability	295:312	chemical stability	295:312	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	2	39	theme	products	365:372	arg1	properties					328:337	organoleptic properties	315:337	organoleptic properties	315:337	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	0	40	theme	orange	39:44	arg1	oil					56:58	cold-pressed orange essential oil	26:58	cold-pressed orange essential oil	26:58	Chitosan nanoemulsions of cold-pressed orange essential oil to preserve fruit juices.					
32659617	2	41	theme	chemical	295:302	arg1	stability					304:312	chemical stability	295:312	chemical stability	295:312	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	9	42	theme	lethal	1459:1464	arg1	effects					1466:1472	synergistic lethal effects	1447:1472	synergistic lethal effects	1447:1472	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	6	43	from	terms	1014:1018	arg1	suspensions					962:972	suspensions	962:972	suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	962:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	6	43	from	terms	1014:1018	arg1	combination					1049:1059	combination	1049:1059	combination with mild heat (52 °C)	1049:1082	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	7	44	theme	greater	1144:1150	arg1	activity					1165:1172	a greater bactericidal activity	1142:1172	a greater bactericidal activity than s-CSEO	1142:1184	cn-CSEO displayed a greater bactericidal activity than s-CSEO at pH 7.0 and pH 4.0.					
32659617	11	45	theme	promising	1732:1740	arg1	alternative					1862:1872	an advantageous alternative	1846:1872	an advantageous alternative	1846:1872	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	11	45	theme	promising	1732:1740	arg1	carrier					1742:1748	a promising carrier	1730:1748	a promising carrier for lipophilic substances	1730:1774	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	8	46	from	validation	1212:1221	arg1	juices					1232:1237	fruit juices	1226:1237	fruit juices	1226:1237	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	11	47	with	encapsulation	1781:1793	arg1	nanoemulsions					1816:1828	chitosan nanoemulsions	1807:1828	chitosan nanoemulsions	1807:1828	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	6	48	from	CSEO	977:980	arg1	terms					1014:1018	terms	1014:1018	terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	1014:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	3	49	theme	antimicrobial	485:497	arg1	activity					499:506	its antimicrobial activity	481:506	its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices	481:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	6	50	theme	heat	1071:1074	arg1	52 °C					1077:1081	mild heat (52 °C)	1066:1082	mild heat (52 °C)	1066:1082	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	11	51	theme	chitosan	1807:1814	arg1	nanoemulsions					1816:1828	chitosan nanoemulsions	1807:1828	chitosan nanoemulsions	1807:1828	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	2	52	theme	valid	269:273	arg1	Nanoencapsulation					237:253	Nanoencapsulation	237:253	Nanoencapsulation	237:253	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	2	52	theme	valid	269:273	arg1	strategy					275:282	a valid strategy	267:282	a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products	267:372	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	8	53	from	effect	1271:1276	arg1	comparison					1292:1301	comparison	1292:1301	comparison with s-CSEO	1292:1313	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	4	54	theme	CSEO	606:609	arg1	composition					611:621	CSEO composition	606:621	CSEO composition	606:621	CSEO composition was analyzed by GC-MS, and 19 components were identified, with limonene as the predominant constituent (95.1%).					
32659617	6	55	from	suspensions	962:972	arg1	terms					1014:1018	terms	1014:1018	terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	1014:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	1	56	theme	cold	169:172	arg1	pressing					174:181	cold pressing	169:181	cold pressing	169:181	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	6	57	theme	antibacterial	1023:1035	arg1	activity					1037:1044	antibacterial activity	1023:1044	antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	1023:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	5	58	theme	low	762:764	arg1	conditions					772:781	low shear conditions	762:781	low shear conditions	762:781	cn-CSEO was prepared under low shear conditions and characterized according to droplet size (<60 nm) and polydispersity index (<0.260 nm).					
32659617	9	59	theme	cycles	1546:1551	arg1	inactivation					1516:1527	the inactivation	1512:1527	the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells	1512:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	11	60	theme	lipophilic	1754:1763	arg1	substances					1765:1774	lipophilic substances	1754:1774	lipophilic substances	1754:1774	Therefore, as a promising carrier for lipophilic substances, the encapsulation of EOs with chitosan nanoemulsions might represent an advantageous alternative when combined with mild heat to preserve fruit juices.					
32659617	6	61	with	combination	1049:1059	arg1	52 °C					1077:1081	mild heat (52 °C)	1066:1082	mild heat (52 °C)	1066:1082	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	9	62	theme	E.	1556:1557	arg1	O157					1564:1567	E. coli O157	1556:1567	E. coli O157:H7 Sakai cells	1556:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	8	63	theme	apple	1347:1351	arg1	juice					1353:1357	apple juice	1347:1357	apple juice	1347:1357	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	8	64	with	comparison	1292:1301	arg1	s-CSEO					1308:1313	s-CSEO	1308:1313	s-CSEO	1308:1313	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	3	65	theme	sensorial	554:562	arg1	acceptability					564:576	its sensorial acceptability	550:576	its sensorial acceptability in orange and apple juices	550:603	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	9	66	theme	O157	1564:1567	arg1	cells					1578:1582	E. coli O157:H7 Sakai cells	1556:1582	E. coli O157:H7 Sakai cells	1556:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	8	67	from	heat	1339:1342	arg1	juice					1353:1357	apple juice	1347:1357	apple juice	1347:1357	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	6	68	theme	4 ± 2 °C.	925:933	arg1	cn-CSEO					935:941	4 ± 2 °C. cn-CSEO	925:941	4 ± 2 °C. cn-CSEO	925:941	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	1	69	theme	Citrus	143:148	arg1	CSEO					160:163	CSEO	160:163	CSEO	160:163	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	1	69	theme	Citrus	143:148	arg1	sinensis					150:157	Citrus sinensis	143:157	Citrus sinensis (CSEO)	143:164	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	9	70	theme	cells	1578:1582	arg1	cycles					1546:1551	up to 5 Log10 cycles	1532:1551	up to 5 Log10 cycles of E. coli O157:H7 Sakai cells	1532:1582	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	8	71	theme	orange	1371:1376	arg1	juice					1378:1382	orange juice	1371:1382	orange juice	1371:1382	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	6	72	from	cn-CSEO	935:941	arg1	stable					893:898	stable	893:898	stable	893:898	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	8	73	theme	fruit	1226:1230	arg1	juices					1232:1237	fruit juices	1226:1237	fruit juices	1226:1237	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	1	74	theme	Sweet	86:90	arg1	orange					92:97	Sweet orange	86:97	Sweet orange essential oil	86:111	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	6	75	dep	CSEO	977:980	arg1	0.2 μL					992:997	0.2 μL	992:997	0.2 μL of CSEO/mL	992:1008	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	6	76	from	activity	1037:1044	arg1	combination					1049:1059	combination	1049:1059	combination with mild heat (52 °C)	1049:1082	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	3	77	theme	present	382:388	arg1	study					390:394	the present study	378:394	the present study	378:394	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	0	78	theme	cold-pressed	26:37	arg1	oil					56:58	cold-pressed orange essential oil	26:58	cold-pressed orange essential oil	26:58	Chitosan nanoemulsions of cold-pressed orange essential oil to preserve fruit juices.					
32659617	1	79	theme	essential	99:107	arg1	product					207:213	a valuable product	196:213	a valuable product by the food industry	196:234	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	1	79	theme	essential	99:107	arg1	oil					109:111	Sweet orange essential oil	86:111	Sweet orange essential oil	86:111	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	8	80	theme	improved	1249:1256	arg1	effect					1271:1276	an improved bactericidal effect	1246:1276	an improved bactericidal effect of cn-CSEO in comparison with s-CSEO	1246:1313	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	6	81	from	combination	1049:1059	arg1	terms					1014:1018	terms	1014:1018	terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai	1014:1121	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	3	82	theme	chitosan	423:430	arg1	cn					447:448	cn	447:448	cn	447:448	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	3	82	theme	chitosan	423:430	arg1	nanoemulsions					432:444	chitosan nanoemulsions	423:444	chitosan nanoemulsions (cn)	423:449	In the present study we encapsulated CSEO using chitosan nanoemulsions (cn) as nanocarrier, and evaluated its antimicrobial activity in combination with mild heat, as well as its sensorial acceptability in orange and apple juices.					
32659617	0	83	theme	essential	46:54	arg1	oil					56:58	cold-pressed orange essential oil	26:58	cold-pressed orange essential oil	26:58	Chitosan nanoemulsions of cold-pressed orange essential oil to preserve fruit juices.					
32659617	9	84	theme	heat	1434:1437	arg1	combination					1405:1415	the combination	1401:1415	the combination of CSEO and mild heat	1401:1437	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	2	85	theme	EO-based	356:363	arg1	products					365:372	EO-based products	356:372	EO-based products	356:372	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	9	86	theme	synergistic	1447:1457	arg1	effects					1466:1472	synergistic lethal effects	1447:1472	synergistic lethal effects	1447:1472	In both juices, the combination of CSEO and mild heat exerted synergistic lethal effects, reducing the treatment time to cause the inactivation of up to 5 Log10 cycles of E. coli O157:H7 Sakai cells.					
32659617	7	87	theme	bactericidal	1152:1163	arg1	activity					1165:1172	a greater bactericidal activity	1142:1172	a greater bactericidal activity than s-CSEO	1142:1184	cn-CSEO displayed a greater bactericidal activity than s-CSEO at pH 7.0 and pH 4.0.					
32659617	8	88	theme	cn-CSEO	1281:1287	arg1	effect					1271:1276	an improved bactericidal effect	1246:1276	an improved bactericidal effect of cn-CSEO in comparison with s-CSEO	1246:1313	The validation in fruit juices showed an improved bactericidal effect of cn-CSEO in comparison with s-CSEO when combined with mild heat in apple juice, but not in orange juice.					
32659617	1	89	used	used	188:191	arg2	oil					109:111	Sweet orange essential oil	86:111	Sweet orange essential oil	86:111	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	1	89	used	used	188:191	arg2	product					207:213	a valuable product	196:213	a valuable product by the food industry	196:234	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	6	90	theme	CSEO/mL	1002:1008	arg1	0.2 μL					992:997	0.2 μL	992:997	0.2 μL of CSEO/mL	992:1008	Nanoemulsions were stable for at least 3 months at 4 ± 2 °C. cn-CSEO were compared with suspensions of CSEO (s-CSEO) (0.2 μL of CSEO/mL) in terms of antibacterial activity in combination with mild heat (52 °C) against Escherichia coli O157:H7 Sakai.					
32659617	2	91	theme	organoleptic	315:326	arg1	properties					328:337	organoleptic properties	315:337	organoleptic properties	315:337	Nanoencapsulation is known as a valid strategy to improve chemical stability, organoleptic properties, and delivery of EO-based products.					
32659617	1	92	theme	valuable	198:205	arg1	product					207:213	a valuable product	196:213	a valuable product by the food industry	196:234	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
32659617	1	92	theme	valuable	198:205	arg1	oil					109:111	Sweet orange essential oil	86:111	Sweet orange essential oil	86:111	Sweet orange essential oil is obtained from the peels of Citrus sinensis (CSEO) by cold pressing, and used as a valuable product by the food industry.					
33955286	6	0	dep	R.	730:731	arg1	extracts					769:776	ficaria aerial parts extracts	748:776	ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL)	748:827	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	0	dep	R.	730:731	arg1	84.55 ± 3.40 µg/mL					809:826	84.55 ± 3.40 µg/mL	809:826	84.55 ± 3.40 µg/mL	809:826	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	0	dep	R.	730:731	arg1	IC50 = 51.61 ± 3.12 µg/mL					779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	0	dep	R.	730:731	arg1	sardous					733:739	R. sardous	730:739	R. sardous	730:739	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	4	1	theme	in	336:337	arg1	concentration					393:405	nitrite concentration	385:405	nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line	385:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	4	1	theme	in	336:337	arg1	properties					356:365	The in vitro inhibitory properties	332:365	The in vitro inhibitory properties	332:365	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	8	2	theme	NO	1100:1101	arg1	production					1086:1095	excessive production	1076:1095	excessive production of NO	1076:1101	The results suggest that Ranunculus hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.					
33955286	4	3	theme	RAW	425:427	arg1	line					451:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	1	4	theme	70	166:167	arg1	%					168:168	%	168:168	%	168:168	Aerial parts and roots from three Ranunculus species were extracted with 70% ethanol.					
33955286	8	5	theme	excessive	1076:1084	arg1	production					1086:1095	excessive production	1076:1095	excessive production of NO	1076:1101	The results suggest that Ranunculus hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.					
33955286	4	6	theme	LPS-stimulated	410:423	arg1	line					451:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	6	7	theme	aerial	756:761	arg1	extracts					769:776	ficaria aerial parts extracts	748:776	ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL)	748:827	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	7	theme	aerial	756:761	arg1	84.55 ± 3.40 µg/mL					809:826	84.55 ± 3.40 µg/mL	809:826	84.55 ± 3.40 µg/mL	809:826	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	7	theme	aerial	756:761	arg1	IC50 = 51.61 ± 3.12 µg/mL					779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	4	8	theme	macrophage	435:444	arg1	line					451:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	5	9	theme	R.	530:531	arg1	sample					592:597	the most interesting sample	571:597	the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL)	571:625	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	9	theme	R.	530:531	arg1	extract					557:563	R. sceleratus aerial parts extract	530:563	R. sceleratus aerial parts extract	530:563	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	8	10	theme	Ranunculus	934:943	arg1	extracts					960:967	Ranunculus hydroalcoholic extracts	934:967	Ranunculus hydroalcoholic extracts	934:967	The results suggest that Ranunculus hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.					
33955286	6	11	theme	ficaria	748:754	arg1	extracts					769:776	ficaria aerial parts extracts	748:776	ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL)	748:827	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	11	theme	ficaria	748:754	arg1	84.55 ± 3.40 µg/mL					809:826	84.55 ± 3.40 µg/mL	809:826	84.55 ± 3.40 µg/mL	809:826	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	11	theme	ficaria	748:754	arg1	IC50 = 51.61 ± 3.12 µg/mL					779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	5	12	theme	compound	664:671	arg1	indomethacin					673:684	the reference compound indomethacin	650:684	the reference compound indomethacin	650:684	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	13	theme	reference	654:662	arg1	indomethacin					673:684	the reference compound indomethacin	650:684	the reference compound indomethacin	650:684	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	4	14	dep	in	336:337	arg1	vitro					339:343	vitro	339:343	vitro	339:343	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	1	15	theme	%	168:168	arg1	ethanol					170:176	70% ethanol	166:176	70% ethanol	166:176	Aerial parts and roots from three Ranunculus species were extracted with 70% ethanol.					
33955286	0	16	from	production	41:50	arg1	macrophages					80:90	LPS-stimulated RAW 264.7 macrophages	55:90	LPS-stimulated RAW 264.7 macrophages	55:90	Ranunculus species suppress nitric oxide production in LPS-stimulated RAW 264.7 macrophages.					
33955286	4	17	theme	nitrite	385:391	arg1	concentration					393:405	nitrite concentration	385:405	nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line	385:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	4	17	theme	nitrite	385:391	arg1	properties					356:365	The in vitro inhibitory properties	332:365	The in vitro inhibitory properties	332:365	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	5	18	theme	aerial	544:549	arg1	sample					592:597	the most interesting sample	571:597	the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL)	571:625	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	18	theme	aerial	544:549	arg1	extract					557:563	R. sceleratus aerial parts extract	530:563	R. sceleratus aerial parts extract	530:563	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	1	19	from	species	138:144	arg1	parts					100:104	Aerial parts	93:104	Aerial parts	93:104	Aerial parts and roots from three Ranunculus species were extracted with 70% ethanol.					
33955286	1	19	from	species	138:144	arg1	roots					110:114	roots	110:114	roots	110:114	Aerial parts and roots from three Ranunculus species were extracted with 70% ethanol.					
33955286	1	20	theme	Aerial	93:98	arg1	parts					100:104	Aerial parts	93:104	Aerial parts	93:104	Aerial parts and roots from three Ranunculus species were extracted with 70% ethanol.					
33955286	5	21	theme	parts	551:555	arg1	sample					592:597	the most interesting sample	571:597	the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL)	571:625	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	21	theme	parts	551:555	arg1	extract					557:563	R. sceleratus aerial parts extract	530:563	R. sceleratus aerial parts extract	530:563	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	0	22	theme	Ranunculus	0:9	arg1	species					11:17	Ranunculus species	0:17	Ranunculus species	0:17	Ranunculus species suppress nitric oxide production in LPS-stimulated RAW 264.7 macrophages.					
33955286	4	23	from	concentration	393:405	arg1	line					451:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	5	24	theme	inhibitory	505:514	arg1	effects					516:522	concentration-dependent inhibitory effects	481:522	concentration-dependent inhibitory effects	481:522	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	0	25	theme	nitric	28:33	arg1	oxide					35:39	nitric oxide	28:39	nitric oxide production in LPS-stimulated RAW 264.7 macrophages	28:90	Ranunculus species suppress nitric oxide production in LPS-stimulated RAW 264.7 macrophages.					
33955286	7	26	theme	root	894:897	arg1	extracts					899:906	the root extracts	890:906	the root extracts	890:906	A lesser but noteworthy potential was also demonstrated for the root extracts.					
33955286	7	27	theme	noteworthy	843:852	arg1	potential					854:862	A lesser but noteworthy potential	830:862	A lesser but noteworthy potential	830:862	A lesser but noteworthy potential was also demonstrated for the root extracts.					
33955286	3	28	theme	flavonoid	293:301	arg1	contents					303:310	The total phenolic and flavonoid contents	270:310	contents	303:310	The total phenolic and flavonoid contents were also assessed.					
33955286	8	29	theme	inflammatory	1042:1053	arg1	diseases					1055:1062	inflammatory diseases	1042:1062	inflammatory diseases mediated by excessive production of NO	1042:1101	The results suggest that Ranunculus hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.					
33955286	5	30	theme	sceleratus	533:542	arg1	sample					592:597	the most interesting sample	571:597	the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL)	571:625	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	30	theme	sceleratus	533:542	arg1	extract					557:563	R. sceleratus aerial parts extract	530:563	R. sceleratus aerial parts extract	530:563	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	2	31	theme	multivariate	242:253	arg1	analysis					260:267	GC-MS and multivariate data analysis	232:267	analysis	260:267	The phytochemical composition was investigated using GC-MS and multivariate data analysis.					
33955286	6	32	dep	extracts	769:776	arg1	extracts					769:776	ficaria aerial parts extracts	748:776	ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL)	748:827	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	32	dep	extracts	769:776	arg1	84.55 ± 3.40 µg/mL					809:826	84.55 ± 3.40 µg/mL	809:826	84.55 ± 3.40 µg/mL	809:826	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	32	dep	extracts	769:776	arg1	IC50 = 51.61 ± 3.12 µg/mL					779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	0	33	theme	oxide	35:39	arg1	production					41:50	nitric oxide production	28:50	nitric oxide production in LPS-stimulated RAW 264.7 macrophages	28:90	Ranunculus species suppress nitric oxide production in LPS-stimulated RAW 264.7 macrophages.					
33955286	7	34	theme	lesser	832:837	arg1	potential					854:862	A lesser but noteworthy potential	830:862	A lesser but noteworthy potential	830:862	A lesser but noteworthy potential was also demonstrated for the root extracts.					
33955286	5	35	theme	interesting	580:590	arg1	sample					592:597	the most interesting sample	571:597	the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL)	571:625	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	35	theme	interesting	580:590	arg1	extract					557:563	R. sceleratus aerial parts extract	530:563	R. sceleratus aerial parts extract	530:563	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	5	35	theme	interesting	580:590	arg1	IC50 = 22.08 ± 1.32 µg/mL					600:624	IC50 = 22.08 ± 1.32 µg/mL	600:624	IC50 = 22.08 ± 1.32 µg/mL	600:624	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	0	36	theme	LPS-stimulated	55:68	arg1	macrophages					80:90	LPS-stimulated RAW 264.7 macrophages	55:90	LPS-stimulated RAW 264.7 macrophages	55:90	Ranunculus species suppress nitric oxide production in LPS-stimulated RAW 264.7 macrophages.					
33955286	2	37	theme	GC-MS	232:236	arg1	analysis					260:267	GC-MS and multivariate data analysis	232:267	analysis	260:267	The phytochemical composition was investigated using GC-MS and multivariate data analysis.					
33955286	4	38	theme	inhibitory	345:354	arg1	concentration					393:405	nitrite concentration	385:405	nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line	385:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	4	38	theme	inhibitory	345:354	arg1	properties					356:365	The in vitro inhibitory properties	332:365	The in vitro inhibitory properties	332:365	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	4	39	theme	cell	446:449	arg1	line					451:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	LPS-stimulated RAW 264.7 macrophage cell line	410:454	The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line.					
33955286	1	40	theme	Ranunculus	127:136	arg1	species					138:144	three Ranunculus species	121:144	three Ranunculus species	121:144	Aerial parts and roots from three Ranunculus species were extracted with 70% ethanol.					
33955286	8	41	theme	nitrite	989:995	arg1	accumulation					997:1008	nitrite accumulation	989:1008	nitrite accumulation	989:1008	The results suggest that Ranunculus hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.					
33955286	6	42	theme	good	694:697	arg1	activity					699:706	A very good activity	687:706	A very good activity	687:706	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	8	43	theme	hydroalcoholic	945:958	arg1	extracts					960:967	Ranunculus hydroalcoholic extracts	934:967	Ranunculus hydroalcoholic extracts	934:967	The results suggest that Ranunculus hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.					
33955286	2	44	theme	phytochemical	183:195	arg1	composition					197:207	The phytochemical composition	179:207	The phytochemical composition	179:207	The phytochemical composition was investigated using GC-MS and multivariate data analysis.					
33955286	5	45	theme	concentration-dependent	481:503	arg1	effects					516:522	concentration-dependent inhibitory effects	481:522	concentration-dependent inhibitory effects	481:522	All the samples induced concentration-dependent inhibitory effects, with R. sceleratus aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin.					
33955286	0	46	theme	RAW	70:72	arg1	macrophages					80:90	LPS-stimulated RAW 264.7 macrophages	55:90	LPS-stimulated RAW 264.7 macrophages	55:90	Ranunculus species suppress nitric oxide production in LPS-stimulated RAW 264.7 macrophages.					
33955286	2	47	theme	data	255:258	arg1	analysis					260:267	GC-MS and multivariate data analysis	232:267	analysis	260:267	The phytochemical composition was investigated using GC-MS and multivariate data analysis.					
33955286	6	48	theme	parts	763:767	arg1	extracts					769:776	ficaria aerial parts extracts	748:776	ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL)	748:827	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	48	theme	parts	763:767	arg1	84.55 ± 3.40 µg/mL					809:826	84.55 ± 3.40 µg/mL	809:826	84.55 ± 3.40 µg/mL	809:826	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
33955286	6	48	theme	parts	763:767	arg1	IC50 = 51.61 ± 3.12 µg/mL					779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	IC50 = 51.61 ± 3.12 µg/mL	779:803	A very good activity was also observed for R. sardous and R. ficaria aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL).					
32791265	0	0	theme	alcohol	92:98	arg1	activity					23:30	Enhanced antimicrobial activity	0:30	Enhanced antimicrobial activity	0:30	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	0	0	theme	alcohol	92:98	arg1	release					60:66	pH-responsive sustained release	36:66	pH-responsive sustained release of chitosan/poly (vinyl alcohol)	36:99	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	2	1	theme	nanofibrous	553:563	arg1	membrane					565:572	the nanofibrous membrane	549:572	the nanofibrous membrane	549:572	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	3	2	theme	nanofibrous	694:704	arg1	membranes					706:714	nanofibrous membranes	694:714	nanofibrous membranes	694:714	The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli.					
32791265	8	3	theme	long-lasting	1341:1352	arg1	efficacy					1354:1361	long-lasting efficacy	1341:1361	long-lasting efficacy	1341:1361	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	6	4	with	loading	1091:1097	arg1	Alli					1104:1107	Alli	1104:1107	Alli	1104:1107	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	4	5	theme	nice	849:852	arg1	activity					868:875	nice antibacterial activity	849:875	nice antibacterial activity	849:875	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	0	6	theme	vinyl	86:90	arg1	alcohol					92:98	chitosan/poly (vinyl alcohol)	71:99	chitosan/poly (vinyl alcohol)	71:99	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	4	7	theme	antibacterial	854:866	arg1	activity					868:875	nice antibacterial activity	849:875	nice antibacterial activity	849:875	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	4	8	contain	had	845:847	arg2	activity					868:875	nice antibacterial activity	849:875	nice antibacterial activity	849:875	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	4	8	contain	had	845:847	arg1	membrane					814:821	the CS/PVA/Alli nanofibrous membrane	786:821	the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO	786:837	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	1	9	theme	chitosan	229:236	arg1	oxide					276:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide	229:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	7	10	theme	essential	1171:1179	arg1	characteristics					1181:1195	The essential characteristics	1167:1195	The essential characteristics of nanofibrous membranes	1167:1220	The essential characteristics of nanofibrous membranes were evaluated by SEM, FTIR, XRD.					
32791265	8	11	theme	antibacterial	1439:1451	arg1	dressing					1459:1466	promising antibacterial wound dressing	1429:1466	promising antibacterial wound dressing	1429:1466	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	6	12	theme	moisture-retention	1138:1155	arg1	capacity					1157:1164	moisture-retention capacity	1138:1164	moisture-retention capacity	1138:1164	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	8	13	theme	strong	1307:1312	arg1	activity					1328:1335	a strong antimicrobial activity	1305:1335	a strong antimicrobial activity	1305:1335	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	8	14	theme	natural	1381:1387	arg1	membranes					1401:1409	the developed natural nanofibrous membranes	1367:1409	the developed natural nanofibrous membranes	1367:1409	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	3	15	theme	membranes	706:714	arg1	activity					682:689	the antibacterial activity	664:689	the antibacterial activity of nanofibrous membranes	664:714	The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli.					
32791265	2	16	theme	GO	543:544	arg1	content					532:538	the content	528:538	the content of GO in the nanofibrous membrane	528:572	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	2	17	from	content	532:538	arg1	membrane					565:572	the nanofibrous membrane	549:572	the nanofibrous membrane	549:572	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	0	18	theme	nanofibrous	116:126	arg1	loading					137:143	oxide nanofibrous membrane loading	110:143	oxide nanofibrous membrane loading with allicin	110:156	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	1	19	dep	oxide	276:280	arg1	GO					283:284	GO	283:284	GO	283:284	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	6	20	theme	nanofibrous	1070:1080	arg1	loading					1091:1097	The CS/PVA/GO nanofibrous membrane loading	1056:1097	The CS/PVA/GO nanofibrous membrane loading with Alli	1056:1107	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	1	21	theme	natural	171:177	arg1	extract					179:185	the natural extract	167:185	the natural extract of garlic, allicin (Alli),	167:212	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	0	22	theme	oxide	110:114	arg1	loading					137:143	oxide nanofibrous membrane loading	110:143	oxide nanofibrous membrane loading with allicin	110:156	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	4	23	theme	0.1 wt	828:833	arg1	%					834:834	0.1 wt% GO	828:837	0.1 wt% GO	828:837	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	4	24	theme	nanofibrous	753:763	arg1	membrane					765:772	the nanofibrous membrane	749:772	the nanofibrous membrane without GO	749:783	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	6	25	theme	CS/PVA/GO	1060:1068	arg1	loading					1091:1097	The CS/PVA/GO nanofibrous membrane loading	1056:1097	The CS/PVA/GO nanofibrous membrane loading with Alli	1056:1107	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	0	26	theme	antimicrobial	9:21	arg1	activity					23:30	Enhanced antimicrobial activity	0:30	Enhanced antimicrobial activity	0:30	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	1	27	theme	nanofibrous	313:323	arg1	membranes					325:333	the nanofibrous membranes	309:333	the nanofibrous membranes with strong antibacterial activity and sustained-release properties	309:401	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	5	28	theme	nanofibrous	916:926	arg1	membranes					928:936	nanofibrous membranes	916:936	nanofibrous membranes	916:936	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	8	29	theme	above	1260:1264	arg1	results					1266:1272	The above results	1256:1272	The above results	1256:1272	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	0	30	theme	Enhanced	0:7	arg1	activity					23:30	Enhanced antimicrobial activity	0:30	Enhanced antimicrobial activity	0:30	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	5	31	theme	membranes	928:936	arg1	angle					907:911	The water contact angle	889:911	The water contact angle of nanofibrous membranes	889:936	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	1	32	theme	garlic	190:195	arg1	extract					179:185	the natural extract	167:185	the natural extract of garlic, allicin (Alli),	167:212	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	4	33	dep	%	834:834	arg1	GO					836:837	GO	836:837	GO	836:837	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	1	34	theme	/polyvinyl	242:251	arg1	oxide					276:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide	229:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	2	35	dep	rate	482:485	arg1	the					470:472	the	470:472	the	470:472	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	8	36	theme	developed	1371:1379	arg1	membranes					1401:1409	the developed natural nanofibrous membranes	1367:1409	the developed natural nanofibrous membranes	1367:1409	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	1	37	theme	alcohol	253:259	arg1	oxide					276:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide	229:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	0	38	theme	membrane	128:135	arg1	loading					137:143	oxide nanofibrous membrane loading	110:143	oxide nanofibrous membrane loading with allicin	110:156	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	6	39	contain	had	1109:1111	arg2	hygroscopicity					1119:1132	great hygroscopicity	1113:1132	great hygroscopicity	1113:1132	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	6	39	contain	had	1109:1111	arg1	loading					1091:1097	The CS/PVA/GO nanofibrous membrane loading	1056:1097	The CS/PVA/GO nanofibrous membrane loading with Alli	1056:1107	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	6	39	contain	had	1109:1111	arg2	capacity					1157:1164	moisture-retention capacity	1138:1164	moisture-retention capacity	1138:1164	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	6	40	theme	membrane	1082:1089	arg1	loading					1091:1097	The CS/PVA/GO nanofibrous membrane loading	1056:1097	The CS/PVA/GO nanofibrous membrane loading with Alli	1056:1107	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	1	41	with	membranes	325:333	arg1	activity					361:368	strong antibacterial activity	340:368	strong antibacterial activity	340:368	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	1	41	with	membranes	325:333	arg1	properties					392:401	sustained-release properties	374:401	sustained-release properties	374:401	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	0	42	theme	sustained	50:58	arg1	release					60:66	pH-responsive sustained release	36:66	pH-responsive sustained release of chitosan/poly (vinyl alcohol)	36:99	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	7	43	theme	membranes	1212:1220	arg1	characteristics					1181:1195	The essential characteristics	1167:1195	The essential characteristics of nanofibrous membranes	1167:1220	The essential characteristics of nanofibrous membranes were evaluated by SEM, FTIR, XRD.					
32791265	7	44	theme	nanofibrous	1200:1210	arg1	membranes					1212:1220	nanofibrous membranes	1200:1220	nanofibrous membranes	1200:1220	The essential characteristics of nanofibrous membranes were evaluated by SEM, FTIR, XRD.					
32791265	4	45	theme	nanofibrous	802:812	arg1	membrane					814:821	the CS/PVA/Alli nanofibrous membrane	786:821	the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO	786:837	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	5	46	dep	showed	1000:1005	arg1	had					1032:1034	had	1032:1034	showed the nanofibrous membrane had highly hydrophobic	1000:1053	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	0	47	theme	pH-responsive	36:48	arg1	release					60:66	pH-responsive sustained release	36:66	pH-responsive sustained release of chitosan/poly (vinyl alcohol)	36:99	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	1	48	theme	PVA	262:264	arg1	oxide					276:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide	229:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	2	49	theme	release	445:451	arg1	test					453:456	Vitro Alli release test	434:456	Vitro Alli release test	434:456	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	1	50	theme	strong	340:345	arg1	activity					361:368	strong antibacterial activity	340:368	strong antibacterial activity	340:368	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	4	51	theme	CS/PVA/Alli	790:800	arg1	membrane					814:821	the CS/PVA/Alli nanofibrous membrane	786:821	the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO	786:837	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	2	52	theme	Alli	501:504	arg1	Alli					501:504	Alli	501:504	Alli	501:504	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	2	52	theme	Alli	501:504	arg1	amount					491:496	amount	491:496	amount	491:496	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	2	52	theme	Alli	501:504	arg1	rate					482:485	release rate	474:485	release rate	474:485	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	2	53	theme	Alli	440:443	arg1	test					453:456	Vitro Alli release test	434:456	Vitro Alli release test	434:456	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	2	54	from	membrane	565:572	arg1	content					532:538	the content	528:538	the content of GO in the nanofibrous membrane	528:572	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	1	55	theme	antibacterial	347:359	arg1	activity					361:368	strong antibacterial activity	340:368	strong antibacterial activity	340:368	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	8	56	theme	promising	1429:1437	arg1	dressing					1459:1466	promising antibacterial wound dressing	1429:1466	promising antibacterial wound dressing	1429:1466	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	5	57	theme	water	893:897	arg1	angle					907:911	The water contact angle	889:911	The water contact angle of nanofibrous membranes	889:936	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	2	58	theme	Vitro	434:438	arg1	test					453:456	Vitro Alli release test	434:456	Vitro Alli release test	434:456	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	1	59	theme	/graphene	266:274	arg1	oxide					276:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide	229:280	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	5	60	theme	nanofibrous	1011:1021	arg1	membrane					1023:1030	the nanofibrous membrane	1007:1030	the nanofibrous membrane	1007:1030	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	5	61	theme	contact	899:905	arg1	angle					907:911	The water contact angle	889:911	The water contact angle of nanofibrous membranes	889:936	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	8	62	theme	tissue	1472:1477	arg1	engineering					1479:1489	tissue engineering	1472:1489	tissue engineering	1472:1489	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	1	63	theme	oxide	276:280	arg1	composites					287:296	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites	229:296	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	8	64	theme	wound	1453:1457	arg1	dressing					1459:1466	promising antibacterial wound dressing	1429:1466	promising antibacterial wound dressing	1429:1466	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	5	65	theme	GO	981:982	arg1	addition					969:976	the addition	965:976	the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic	965:1053	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	8	66	theme	nanofibrous	1389:1399	arg1	membranes					1401:1409	the developed natural nanofibrous membranes	1367:1409	the developed natural nanofibrous membranes	1367:1409	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	6	67	theme	great	1113:1117	arg1	hygroscopicity					1119:1132	great hygroscopicity	1113:1132	great hygroscopicity	1113:1132	The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity.					
32791265	3	68	dep	activity	593:600	arg1	test					602:605	test	602:605	test	602:605	The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli.					
32791265	8	69	contain	has	1301:1303	arg2	efficacy					1354:1361	long-lasting efficacy	1341:1361	long-lasting efficacy	1341:1361	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	8	69	contain	has	1301:1303	arg1	membrane					1292:1299	the membrane	1288:1299	the membrane	1288:1299	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	8	69	contain	has	1301:1303	arg2	activity					1328:1335	a strong antimicrobial activity	1305:1335	a strong antimicrobial activity	1305:1335	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32791265	1	70	theme	sustained-release	374:390	arg1	properties					392:401	sustained-release properties	374:401	sustained-release properties	374:401	Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology.					
32791265	3	71	theme	antibacterial	579:591	arg1	activity					593:600	The antibacterial activity test	575:605	The antibacterial activity test against Staphylococcus aureus	575:635	The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli.					
32791265	0	72	with	loading	137:143	arg1	allicin					150:156	allicin	150:156	allicin	150:156	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	4	73	with	membrane	814:821	arg1	%					834:834	0.1 wt% GO	828:837	0.1 wt% GO	828:837	Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h.					
32791265	2	74	theme	release	474:480	arg1	rate					482:485	release rate	474:485	release rate	474:485	Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane.					
32791265	5	75	theme	Alli	988:991	arg1	addition					969:976	the addition	965:976	the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic	965:1053	The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic.					
32791265	3	76	with	loading	716:722	arg1	Alli					729:732	Alli	729:732	Alli	729:732	The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli.					
32791265	0	77	theme	chitosan/poly	71:83	arg1	alcohol					92:98	chitosan/poly (vinyl alcohol)	71:99	chitosan/poly (vinyl alcohol)	71:99	Enhanced antimicrobial activity and pH-responsive sustained release of chitosan/poly (vinyl alcohol)/graphene oxide nanofibrous membrane loading with allicin.					
32791265	3	78	theme	antibacterial	668:680	arg1	activity					682:689	the antibacterial activity	664:689	the antibacterial activity of nanofibrous membranes	664:714	The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli.					
32791265	8	79	theme	antimicrobial	1314:1326	arg1	activity					1328:1335	a strong antimicrobial activity	1305:1335	a strong antimicrobial activity	1305:1335	The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.					
32996271	0	0	theme	blood	73:77	arg1	profile					85:91	blood lipid profile	73:91	blood lipid profile	73:91	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	1	1	theme	Pineapple	138:146	arg1	PS					161:162	PS	161:162	PS	161:162	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	1	theme	Pineapple	138:146	arg1	starch					153:158	Pineapple stem starch	138:158	Pineapple stem starch (PS)	138:163	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	2	theme	starch	208:213	arg1	source					215:220	a new starch source	202:220	a new starch source in concentrate	202:235	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	2	3	theme	corn	454:457	arg1	GC					460:461	ground corn (GC) and ground cassava	447:481	GC	460:461	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	2	3	theme	corn	454:457	arg1	CA					484:485	CA	484:485	CA	484:485	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	1	4	theme	blood	292:296	arg1	profile					298:304	blood profile	292:304	blood profile	292:304	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	3	5	theme	weight	538:543	arg1	gain					545:548	weight gain	538:548	weight gain	538:548	PS feeding improved weight gain and feed conversion ratio without affecting feed intake.					
32996271	5	6	theme	SCFA	784:787	arg1	composition					789:799	SCFA composition	784:799	SCFA composition	784:799	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	1	7	theme	stem	148:151	arg1	PS					161:162	PS	161:162	PS	161:162	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	7	theme	stem	148:151	arg1	starch					153:158	Pineapple stem starch	138:158	Pineapple stem starch (PS)	138:163	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	0	8	from	Effect	0:5	arg1	profile					85:91	blood lipid profile	73:91	blood lipid profile	73:91	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	0	8	from	Effect	0:5	arg1	performance					105:115	growth performance	98:115	growth performance of fattening cattle	98:135	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	0	8	from	Effect	0:5	arg1	fermentation					59:70	rumen microbial fermentation	43:70	rumen microbial fermentation	43:70	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	2	9	theme	%	394:394	arg1	concentrate					396:406	a 40% concentrate	390:406	a 40% concentrate	390:406	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	2	9	theme	%	394:394	arg1	PS					369:370	PS	369:370	PS	369:370	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	4	10	theme	lipid	644:648	arg1	profiles					650:657	blood lipid profiles	638:657	blood lipid profiles	638:657	PS did not obviously influence blood lipid profiles throughout the experiment.					
32996271	0	11	theme	lipid	79:83	arg1	profile					85:91	blood lipid profile	73:91	blood lipid profile	73:91	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	3	12	theme	conversion	559:568	arg1	ratio					570:574	feed conversion ratio	554:574	feed conversion ratio	554:574	PS feeding improved weight gain and feed conversion ratio without affecting feed intake.					
32996271	4	13	theme	blood	638:642	arg1	profiles					650:657	blood lipid profiles	638:657	blood lipid profiles	638:657	PS did not obviously influence blood lipid profiles throughout the experiment.					
32996271	1	14	from	performance	279:289	arg1	study					362:366	a 206-day feeding study	344:366	a 206-day feeding study	344:366	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	5	15	theme	Ruminal	686:692	arg1	concentration					694:706	Ruminal concentration	686:706	Ruminal concentration of total short-chain fatty acids (SCFA)	686:746	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	1	16	theme	rumen	311:315	arg1	parameters					317:326	rumen parameters	311:326	rumen parameters	311:326	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	2	17	theme	same	506:509	arg1	level					511:515	the same level	502:515	the same level	502:515	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	7	18	theme	starch	1069:1074	arg1	source					1076:1081	a useful starch source	1060:1081	a useful starch source for fattening cattle in terms of rumen fermentation and growth performance	1060:1156	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	6	19	theme	PS	968:969	arg1	feeding					971:977	PS feeding	968:977	PS feeding	968:977	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	2	20	theme	40	392:393	arg1	%					394:394	%	394:394	%	394:394	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	2	21	theme	ground	468:473	arg1	cassava					475:481	ground corn (GC) and ground cassava	447:481	cassava	475:481	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	1	22	from	parameters	317:326	arg1	study					362:366	a 206-day feeding study	344:366	a 206-day feeding study	344:366	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	7	23	theme	PS	1054:1055	arg1	potential					1041:1049	the potential	1037:1049	the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance	1037:1156	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	6	24	from	dominant	891:898	arg1	community					923:931	the rumen microbial community	903:931	the rumen microbial community	903:931	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	0	25	theme	stem	20:23	arg1	feeding					32:38	pineapple stem starch feeding	10:38	pineapple stem starch feeding	10:38	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	2	26	with	comparison	431:440	arg1	GC					460:461	ground corn (GC) and ground cassava	447:481	GC	460:461	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	2	26	with	comparison	431:440	arg1	CA					484:485	CA	484:485	CA	484:485	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	2	26	with	comparison	431:440	arg1	cassava					475:481	ground corn (GC) and ground cassava	447:481	cassava	475:481	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	5	27	theme	fatty	729:733	arg1	SCFA					742:745	SCFA	742:745	SCFA	742:745	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	5	27	theme	fatty	729:733	arg1	acids					735:739	total short-chain fatty acids	711:739	total short-chain fatty acids (SCFA)	711:746	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	0	28	theme	pineapple	10:18	arg1	feeding					32:38	pineapple stem starch feeding	10:38	pineapple stem starch feeding	10:38	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	1	29	dep	performance	279:289	arg1	the					268:270	the	268:270	the	268:270	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	0	30	theme	growth	98:103	arg1	performance					105:115	growth performance	98:115	growth performance of fattening cattle	98:135	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	5	31	theme	acids	735:739	arg1	concentration					694:706	Ruminal concentration	686:706	Ruminal concentration of total short-chain fatty acids (SCFA)	686:746	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	0	32	theme	feeding	32:38	arg1	Effect					0:5	Effect	0:5	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.	0:136	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	0	33	theme	fattening	120:128	arg1	cattle					130:135	fattening cattle	120:135	fattening cattle	120:135	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	0	34	theme	starch	25:30	arg1	feeding					32:38	pineapple stem starch feeding	10:38	pineapple stem starch feeding	10:38	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	1	35	from	profile	298:304	arg1	study					362:366	a 206-day feeding study	344:366	a 206-day feeding study	344:366	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	36	theme	steers	334:339	arg1	performance					279:289	growth performance	272:289	growth performance	272:289	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	36	theme	steers	334:339	arg1	profile					298:304	blood profile	292:304	blood profile	292:304	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	36	theme	steers	334:339	arg1	parameters					317:326	rumen parameters	311:326	rumen parameters	311:326	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	5	37	theme	total	711:715	arg1	SCFA					742:745	SCFA	742:745	SCFA	742:745	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	5	37	theme	total	711:715	arg1	acids					735:739	total short-chain fatty acids	711:739	total short-chain fatty acids (SCFA)	711:746	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	2	38	theme	ground	447:452	arg1	GC					460:461	ground corn (GC) and ground cassava	447:481	GC	460:461	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	2	38	theme	ground	447:452	arg1	CA					484:485	CA	484:485	CA	484:485	PS was formulated as a 40% concentrate and fed with forage in comparison with ground corn (GC) and ground cassava (CA) formulated at the same level.					
32996271	0	39	theme	rumen	43:47	arg1	fermentation					59:70	rumen microbial fermentation	43:70	rumen microbial fermentation	43:70	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	6	40	theme	amylolytic	837:846	arg1	group					848:852	Rumen amylolytic group	831:852	Rumen amylolytic group	831:852	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	3	41	theme	PS	518:519	arg1	feeding					521:527	PS feeding	518:527	PS feeding	518:527	PS feeding improved weight gain and feed conversion ratio without affecting feed intake.					
32996271	3	42	theme	feed	594:597	arg1	intake					599:604	feed intake	594:604	feed intake	594:604	PS feeding improved weight gain and feed conversion ratio without affecting feed intake.					
32996271	1	43	from	source	215:220	arg1	concentrate					225:235	concentrate	225:235	concentrate	225:235	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	6	44	theme	Rumen	831:835	arg1	group					848:852	Rumen amylolytic group	831:852	Rumen amylolytic group	831:852	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	0	45	theme	cattle	130:135	arg1	profile					85:91	blood lipid profile	73:91	blood lipid profile	73:91	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	0	45	theme	cattle	130:135	arg1	fermentation					59:70	rumen microbial fermentation	43:70	rumen microbial fermentation	43:70	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	0	45	theme	cattle	130:135	arg1	performance					105:115	growth performance	98:115	growth performance of fattening cattle	98:135	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	3	46	theme	feed	554:557	arg1	ratio					570:574	feed conversion ratio	554:574	feed conversion ratio	554:574	PS feeding improved weight gain and feed conversion ratio without affecting feed intake.					
32996271	6	47	from	community	923:931	arg1	dominant					891:898	dominant	891:898	dominant	891:898	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	1	48	theme	growth	272:277	arg1	performance					279:289	growth performance	272:289	growth performance	272:289	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	1	49	theme	206-day	346:352	arg1	study					362:366	a 206-day feeding study	344:366	a 206-day feeding study	344:366	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	5	50	theme	feeding	816:822	arg1	study					824:828	the feeding study	812:828	the feeding study	812:828	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	1	51	theme	feeding	354:360	arg1	study					362:366	a 206-day feeding study	344:366	a 206-day feeding study	344:366	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
32996271	7	52	theme	useful	1062:1067	arg1	source					1076:1081	a useful starch source	1060:1081	a useful starch source for fattening cattle in terms of rumen fermentation and growth performance	1060:1156	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	6	53	dep	group	848:852	arg1	bromii					879:884	Ruminococcus bromii	866:884	Ruminococcus bromii	866:884	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	6	54	theme	microbial	913:921	arg1	community					923:931	the rumen microbial community	903:931	the rumen microbial community	903:931	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	6	55	theme	increased	945:953	arg1	abundance					955:963	increased abundance	945:963	increased abundance by PS feeding throughout the experiment	945:1003	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	7	56	theme	growth	1139:1144	arg1	performance					1146:1156	growth performance	1139:1156	growth performance	1139:1156	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	7	57	theme	rumen	1116:1120	arg1	fermentation					1122:1133	rumen fermentation	1116:1133	rumen fermentation	1116:1133	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	6	58	theme	rumen	907:911	arg1	community					923:931	the rumen microbial community	903:931	the rumen microbial community	903:931	Rumen amylolytic group, especially Ruminococcus bromii, was dominant in the rumen microbial community, and showed increased abundance by PS feeding throughout the experiment.					
32996271	0	59	theme	microbial	49:57	arg1	fermentation					59:70	rumen microbial fermentation	43:70	rumen microbial fermentation	43:70	Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle.					
32996271	5	60	theme	short-chain	717:727	arg1	SCFA					742:745	SCFA	742:745	SCFA	742:745	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	5	60	theme	short-chain	717:727	arg1	acids					735:739	total short-chain fatty acids	711:739	total short-chain fatty acids (SCFA)	711:746	Ruminal concentration of total short-chain fatty acids (SCFA) increased with PS without affecting SCFA composition throughout the feeding study.					
32996271	7	61	theme	performance	1146:1156	arg1	terms					1107:1111	terms	1107:1111	terms of rumen fermentation and growth performance	1107:1156	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	7	62	theme	fermentation	1122:1133	arg1	terms					1107:1111	terms	1107:1111	terms of rumen fermentation and growth performance	1107:1156	These results clearly indicate the potential of PS as a useful starch source for fattening cattle in terms of rumen fermentation and growth performance.					
32996271	1	63	theme	new	204:206	arg1	source					215:220	a new starch source	202:220	a new starch source in concentrate	202:235	Pineapple stem starch (PS) was evaluated for its suitability as a new starch source in concentrate for fattening cattle, based on the growth performance, blood profile, and rumen parameters of 36 steers in a 206-day feeding study.					
33757496	7	0	theme	stomatal	1694:1701	arg1	conductance					1703:1713	stomatal conductance	1694:1713	stomatal conductance	1694:1713	Trends of δ18Ocellulose differed strongly between plots and indicated much greater reductions of stomatal conductance in grass-rich than dicot-rich communities.					
33757496	4	1	theme	archived	924:931	arg1	herbage					933:939	archived herbage	924:939	archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	924:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	3	2	theme	ca-increase	576:586	arg1	change					600:605	the ca-increase and climate change	572:605	the ca-increase and climate change over the last century	572:627	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	2	3	theme	key	294:296	arg1	player					298:303	a key player	292:303	a key player in these coupled cycles	292:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	3	theme	key	294:296	arg1	conductance					277:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	4	4	theme	independent	760:770	arg1	proxies					772:778	two independent proxies	756:778	two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes	756:852	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	11	5	theme	responsive	2509:2518	arg1	forbs					2520:2524	responsive forbs	2509:2524	responsive forbs	2509:2524	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	3	6	theme	last	616:619	arg1	century					621:627	the last century	612:627	the last century	612:627	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	11	7	from	expansion	2452:2460	arg1	disadvantage					2480:2491	the disadvantage	2476:2491	the disadvantage of the more CO2	2476:2507	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	2	8	theme	vegetation	534:543	arg1	status					547:552	vegetation N status	534:552	vegetation N status	534:552	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	4	9	theme	Grass	996:1000	arg1	Experiment					1002:1011	the Park Grass Experiment	987:1011	the Park Grass Experiment	987:1011	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	10	10	theme	attained	2257:2264	arg1	saturation					2266:2275	attained saturation	2257:2275	attained saturation of C sink activity	2257:2294	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	9	11	theme	soil	2091:2094	arg1	pH					2096:2097	soil pH	2091:2097	soil pH	2091:2097	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	8	12	theme	conductance	1781:1791	arg1	Reductions					1758:1767	Reductions	1758:1767	Reductions of stomatal conductance	1758:1791	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	6	13	theme	published	1367:1375	arg1	sensitivities					1377:1389	published sensitivities	1367:1389	published sensitivities	1367:1389	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	11	14	from	disadvantage	2480:2491	arg1	expansion					2452:2460	the expansion	2448:2460	the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes	2448:2545	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	14	from	disadvantage	2480:2491	arg1	legumes					2539:2545	N-fixing legumes	2530:2545	N-fixing legumes	2530:2545	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	14	from	disadvantage	2480:2491	arg1	grasses					2465:2471	grasses	2465:2471	grasses at the disadvantage of the more CO2	2465:2507	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	10	15	theme	temperate	2228:2236	arg1	grassland					2238:2246	temperate grassland	2228:2246	temperate grassland	2228:2246	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	2	16	theme	Plant	255:259	arg1	player					298:303	a key player	292:303	a key player in these coupled cycles	292:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	16	theme	Plant	255:259	arg1	conductance					277:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	4	17	theme	communities	972:982	arg1	range					953:957	a wide range	946:957	a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	946:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	18	theme	stomatal	825:832	arg1	canopy-scale					811:822	growing season-integrating canopy-scale	784:822	growing season-integrating canopy-scale	784:822	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	18	theme	stomatal	825:832	arg1	changes					846:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	6	19	theme	conductance	1562:1572	arg1	changes					1574:1580	stomatal conductance changes	1553:1580	stomatal conductance changes at Park Grass	1553:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	10	20	dep	CONCLUSIONS	2179:2189	arg1	indicate					2200:2207	indicate	2200:2207	indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago	2200:2320	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	9	21	theme	fertilizer	2120:2129	arg1	supply					2131:2136	nitrogen fertilizer supply	2111:2136	nitrogen fertilizer supply which promoted the abundance of grasses	2111:2176	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	8	22	theme	yield	1827:1831	arg1	trends					1833:1838	yield trends	1827:1838	yield trends	1827:1838	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	6	23	theme	environment	1443:1453	arg1	range					1400:1404	a range	1398:1404	a range of contemporary field and controlled environment studies	1398:1461	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	1	24	theme	water	181:185	arg1	cycles					205:210	carbon (C), water, and nitrogen (N) cycles	169:210	carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes	169:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	10	25	theme	sink	2282:2285	arg1	activity					2287:2294	C sink activity	2280:2294	C sink activity	2280:2294	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	3	26	theme	stomatal	658:665	arg1	conductance					667:677	canopy stomatal conductance	651:677	canopy stomatal conductance	651:677	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	7	27	theme	δ18Ocellulose	1607:1619	arg1	Trends					1597:1602	Trends	1597:1602	Trends of δ18Ocellulose	1597:1619	Trends of δ18Ocellulose differed strongly between plots and indicated much greater reductions of stomatal conductance in grass-rich than dicot-rich communities.					
33757496	11	28	theme	Increasing	2323:2332	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	28	theme	Increasing	2323:2332	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	4	29	theme	growing	784:790	arg1	canopy-scale					811:822	growing season-integrating canopy-scale	784:822	growing season-integrating canopy-scale	784:822	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	29	theme	growing	784:790	arg1	changes					846:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	6	30	theme	historical	1524:1533	arg1	reconstruction					1535:1548	historical reconstruction	1524:1548	historical reconstruction of stomatal conductance changes at Park Grass	1524:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	3	31	theme	C	698:698	arg1	processes					706:714	C and N processes	698:714	C and N processes in grassland	698:727	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	8	32	theme	acquisition	1850:1860	arg1	reductions					1813:1822	reductions	1813:1822	reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index	1813:1890	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	3	33	with	links	687:691	arg1	processes					706:714	C and N processes	698:714	C and N processes in grassland	698:727	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	1	34	theme	CO2	133:135	arg1	ca					152:153	ca	152:153	ca	152:153	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	1	34	theme	CO2	133:135	arg1	concentration					137:149	atmospheric CO2 concentration	121:149	atmospheric CO2 concentration (ca)	121:154	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	4	35	from	range	953:957	arg1	Experiment					1002:1011	the Park Grass Experiment	987:1011	the Park Grass Experiment	987:1011	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	35	from	range	953:957	arg1	U.K.					1028:1031	U.K.	1028:1031	U.K.	1028:1031	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	35	from	range	953:957	arg1	herbage					933:939	archived herbage	924:939	archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	924:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	35	from	range	953:957	arg1	changes					1038:1044	changes	1038:1044	changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	1038:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	35	from	range	953:957	arg1	Rothamsted					1016:1025	Rothamsted	1016:1025	Rothamsted (U.K.)	1016:1032	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	6	36	theme	changes	1512:1518	arg1	utility					1487:1493	the utility	1483:1493	the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass	1483:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	11	37	theme	change	2386:2391	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	37	theme	change	2386:2391	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	2	38	theme	gain	411:414	arg1	ratio					400:404	the ratio	396:404	the ratio of C gain by photosynthesis to water loss by transpiration	396:463	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	39	theme	C	409:409	arg1	gain					411:414	C gain	409:414	C gain	409:414	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	1	40	theme	other	229:233	arg1	biomes					247:252	other terrestrial biomes	229:252	other terrestrial biomes	229:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	11	41	theme	effective	2368:2376	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	41	theme	effective	2368:2376	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	9	42	theme	regimes	2079:2085	arg1	independent					2053:2063	independent	2053:2063	independent	2053:2063	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	0	43	theme	last	66:69	arg1	century					71:77	the last century	62:77	the last century	62:77	Stomatal conductance limited the CO2 response of grassland in the last century.					
33757496	2	44	dep	efficiency	384:393	arg1	ratio					400:404	the ratio	396:404	the ratio of C gain by photosynthesis to water loss by transpiration	396:463	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	6	45	theme	δ18Ocellulose	1498:1510	arg1	changes					1512:1518	δ18Ocellulose changes	1498:1518	δ18Ocellulose changes	1498:1518	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	4	46	theme	changes	846:852	arg1	proxies					772:778	two independent proxies	756:778	two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes	756:852	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	47	theme	CO2	1076:1078	arg1	gradient					1094:1101	the CO2 concentration gradient	1072:1101	the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	1072:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	3	48	theme	N	704:704	arg1	processes					706:714	C and N processes	698:714	C and N processes in grassland	698:727	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	6	49	theme	contemporary	1409:1420	arg1	field					1422:1426	contemporary field	1409:1426	contemporary field	1409:1426	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	2	50	theme	N	545:545	arg1	status					547:552	vegetation N status	534:552	vegetation N status	534:552	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	51	theme	use	380:382	arg1	efficiency					384:393	vegetation water use efficiency	363:393	vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration)	363:464	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	11	52	theme	fertilizer	2336:2345	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	52	theme	fertilizer	2336:2345	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	2	53	theme	vegetation	363:372	arg1	efficiency					384:393	vegetation water use efficiency	363:393	vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration)	363:464	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	9	54	theme	different	1976:1984	arg1	degrees					1986:1992	different degrees	1976:1992	different degrees	1976:1992	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	5	55	with	agreement	1220:1228	arg1	hypothesis					1239:1248	the hypothesis	1235:1248	the hypothesis	1235:1248	The two proxies correlated closely (R2 = 0.70), in agreement with the hypothesis.					
33757496	1	56	theme	anthropogenic	95:107	arg1	increase					109:116	The anthropogenic increase	91:116	The anthropogenic increase of atmospheric CO2 concentration (ca)	91:154	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	4	57	theme	δ18O	887:890	arg1	trends					877:882	trends	877:882	trends of δ18O in cellulose (δ18Ocellulose)	877:919	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	58	theme	leaf	1134:1137	arg1	space					1152:1156	the leaf internal gas space	1130:1156	the leaf internal gas space	1130:1156	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	0	59	theme	CO2	33:35	arg1	response					37:44	the CO2 response	29:44	the CO2 response of grassland in the last century	29:77	Stomatal conductance limited the CO2 response of grassland in the last century.					
33757496	7	60	theme	dicot-rich	1734:1743	arg1	communities					1745:1755	dicot-rich communities	1734:1755	dicot-rich communities	1734:1755	Trends of δ18Ocellulose differed strongly between plots and indicated much greater reductions of stomatal conductance in grass-rich than dicot-rich communities.					
33757496	6	61	from	reconstruction	1535:1548	arg1	Grass					1590:1594	Park Grass	1585:1594	Park Grass	1585:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	4	62	theme	gas	1148:1150	arg1	space					1152:1156	the leaf internal gas space	1130:1156	the leaf internal gas space	1130:1156	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	10	63	contain	have	2252:2255	arg1	types					2219:2223	some types	2214:2223	some types of temperate grassland	2214:2246	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	10	63	contain	have	2252:2255	arg2	saturation					2266:2275	attained saturation	2257:2275	attained saturation of C sink activity	2257:2294	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	4	64	from	changes	1038:1044	arg1	range					953:957	a wide range	946:957	a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	946:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	64	from	changes	1038:1044	arg1	communities					972:982	grassland communities	962:982	grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	962:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	9	65	theme	stomatal	2019:2026	arg1	conductance					2028:2038	stomatal conductance	2019:2038	stomatal conductance	2019:2038	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	6	66	theme	estimated	1308:1316	arg1	changes					1339:1345	estimated stomatal conductance changes	1308:1345	estimated stomatal conductance changes	1308:1345	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	4	67	from	trends	877:882	arg1	δ18Ocellulose					906:918	δ18Ocellulose	906:918	δ18Ocellulose	906:918	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	67	from	trends	877:882	arg1	cellulose					895:903	cellulose	895:903	cellulose (δ18Ocellulose)	895:919	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	7	68	theme	conductance	1703:1713	arg1	reductions					1680:1689	much greater reductions	1667:1689	much greater reductions of stomatal conductance in grass-rich	1667:1727	Trends of δ18Ocellulose differed strongly between plots and indicated much greater reductions of stomatal conductance in grass-rich than dicot-rich communities.					
33757496	4	69	theme	grassland	962:970	arg1	communities					972:982	grassland communities	962:982	grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	962:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	11	70	theme	CO2	2505:2507	arg1	disadvantage					2480:2491	the disadvantage	2476:2491	the disadvantage of the more CO2	2476:2507	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	6	71	theme	changes	1297:1303	arg1	sensitivity					1268:1278	the sensitivity	1264:1278	the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes	1264:1345	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	1	72	theme	nitrogen	192:199	arg1	cycles					205:210	carbon (C), water, and nitrogen (N) cycles	169:210	carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes	169:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	9	73	theme	grasses	2170:2176	arg1	abundance					2157:2165	the abundance	2153:2165	the abundance of grasses	2153:2176	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	2	74	from	control	352:358	arg1	cycles					322:327	these coupled cycles	308:327	these coupled cycles	308:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	9	75	theme	pH	2096:2097	arg1	independent					2053:2063	independent	2053:2063	independent	2053:2063	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	11	76	theme	many	2416:2419	arg1	grasslands					2421:2430	many grasslands	2416:2430	many grasslands	2416:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	7	77	theme	greater	1672:1678	arg1	reductions					1680:1689	much greater reductions	1667:1689	much greater reductions of stomatal conductance in grass-rich	1667:1727	Trends of δ18Ocellulose differed strongly between plots and indicated much greater reductions of stomatal conductance in grass-rich than dicot-rich communities.					
33757496	7	78	from	reductions	1680:1689	arg1	grass-rich					1718:1727	grass-rich	1718:1727	grass-rich	1718:1727	Trends of δ18Ocellulose differed strongly between plots and indicated much greater reductions of stomatal conductance in grass-rich than dicot-rich communities.					
33757496	11	79	theme	N-fixing	2530:2537	arg1	legumes					2539:2545	N-fixing legumes	2530:2545	N-fixing legumes	2530:2545	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	9	80	theme	fertilizer	2068:2077	arg1	regimes					2079:2085	fertilizer regimes	2068:2085	fertilizer regimes	2068:2085	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	8	81	theme	stomatal	1772:1779	arg1	conductance					1781:1791	stomatal conductance	1772:1791	stomatal conductance	1772:1791	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	2	82	theme	photosynthetic	486:499	arg1	activity					501:508	photosynthetic activity	486:508	photosynthetic activity	486:508	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	4	83	from	Experiment	1002:1011	arg1	range					953:957	a wide range	946:957	a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	946:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	84	from	Rothamsted	1016:1025	arg1	range					953:957	a wide range	946:957	a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	946:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	84	from	Rothamsted	1016:1025	arg1	communities					972:982	grassland communities	962:982	grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	962:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	10	85	theme	grassland	2238:2246	arg1	types					2219:2223	some types	2214:2223	some types of temperate grassland	2214:2246	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	2	86	from	player	298:303	arg1	cycles					322:327	these coupled cycles	308:327	these coupled cycles	308:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	8	87	theme	nutrition	1876:1884	arg1	index					1886:1890	nitrogen nutrition index	1867:1890	nitrogen nutrition index	1867:1890	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	2	88	theme	canopy	261:266	arg1	player					298:303	a key player	292:303	a key player in these coupled cycles	292:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	88	theme	canopy	261:266	arg1	conductance					277:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	1	89	theme	carbon	169:174	arg1	cycles					205:210	carbon (C), water, and nitrogen (N) cycles	169:210	carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes	169:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	3	90	theme	canopy	651:656	arg1	conductance					667:677	canopy stomatal conductance	651:677	canopy stomatal conductance	651:677	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	1	91	from	cycles	205:210	arg1	grassland					215:223	grassland	215:223	grassland	215:223	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	1	91	from	cycles	205:210	arg1	biomes					247:252	other terrestrial biomes	229:252	other terrestrial biomes	229:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	4	92	theme	season-integrating	792:809	arg1	canopy-scale					811:822	growing season-integrating canopy-scale	784:822	growing season-integrating canopy-scale	784:822	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	92	theme	season-integrating	792:809	arg1	changes					846:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	6	93	theme	changes	1574:1580	arg1	reconstruction					1535:1548	historical reconstruction	1524:1548	historical reconstruction of stomatal conductance changes at Park Grass	1524:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	4	94	theme	Park	991:994	arg1	Experiment					1002:1011	the Park Grass Experiment	987:1011	the Park Grass Experiment	987:1011	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	9	95	theme	nitrogen	2111:2118	arg1	supply					2131:2136	nitrogen fertilizer supply	2111:2136	nitrogen fertilizer supply which promoted the abundance of grasses	2111:2176	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	8	96	theme	trends	1833:1838	arg1	reductions					1813:1822	reductions	1813:1822	reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index	1813:1890	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	6	97	theme	conductance	1327:1337	arg1	changes					1339:1345	estimated stomatal conductance changes	1308:1345	estimated stomatal conductance changes	1308:1345	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	11	98	from	strategy	2404:2411	arg1	grasslands					2421:2430	many grasslands	2416:2430	many grasslands	2416:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	6	99	theme	stomatal	1553:1560	arg1	changes					1574:1580	stomatal conductance changes	1553:1580	stomatal conductance changes at Park Grass	1553:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	8	100	theme	nitrogen	1841:1848	arg1	acquisition					1850:1860	nitrogen acquisition	1841:1860	nitrogen acquisition	1841:1860	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	6	101	theme	controlled	1432:1441	arg1	environment					1443:1453	controlled environment	1432:1453	controlled environment	1432:1453	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	10	102	theme	activity	2287:2294	arg1	saturation					2266:2275	attained saturation	2257:2275	attained saturation of C sink activity	2257:2294	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	4	103	theme	ratio	1053:1057	arg1	U.K.					1028:1031	U.K.	1028:1031	U.K.	1028:1031	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	103	theme	ratio	1053:1057	arg1	changes					1038:1044	changes	1038:1044	changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	1038:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	103	theme	ratio	1053:1057	arg1	Rothamsted					1016:1025	Rothamsted	1016:1025	Rothamsted (U.K.)	1016:1032	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	6	104	theme	field	1422:1426	arg1	range					1400:1404	a range	1398:1404	a range of contemporary field and controlled environment studies	1398:1461	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	1	105	theme	atmospheric	121:131	arg1	ca					152:153	ca	152:153	ca	152:153	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	1	105	theme	atmospheric	121:131	arg1	concentration					137:149	atmospheric CO2 concentration	121:149	atmospheric CO2 concentration (ca)	121:154	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	10	106	theme	C	2280:2280	arg1	activity					2287:2294	C sink activity	2280:2294	C sink activity	2280:2294	CONCLUSIONS Our data indicate that some types of temperate grassland may have attained saturation of C sink activity more than one century ago.					
33757496	11	107	theme	grasses	2465:2471	arg1	expansion					2452:2460	the expansion	2448:2460	the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes	2448:2545	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	108	theme	climate	2378:2384	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	108	theme	climate	2378:2384	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	8	109	theme	nitrogen	1867:1874	arg1	index					1886:1890	nitrogen nutrition index	1867:1890	nitrogen nutrition index	1867:1890	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	1	110	theme	concentration	137:149	arg1	increase					109:116	The anthropogenic increase	91:116	The anthropogenic increase of atmospheric CO2 concentration (ca)	91:154	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	4	111	theme	last	863:866	arg1	century					868:874	the last century	859:874	the last century	859:874	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	112	theme	conductance	834:844	arg1	canopy-scale					811:822	growing season-integrating canopy-scale	784:822	growing season-integrating canopy-scale	784:822	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	4	112	theme	conductance	834:844	arg1	changes					846:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	(growing season-integrating canopy-scale) stomatal conductance changes	783:852	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	8	113	theme	index	1886:1890	arg1	reductions					1813:1822	reductions	1813:1822	reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index	1813:1890	Reductions of stomatal conductance were connected with reductions of yield trends, nitrogen acquisition, and nitrogen nutrition index.					
33757496	6	114	dep	field	1422:1426	arg1	studies					1455:1461	studies	1455:1461	studies	1455:1461	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	11	115	dep	grasses	2465:2471	arg1	forbs					2520:2524	responsive forbs	2509:2524	responsive forbs	2509:2524	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	116	theme	mitigation	2393:2402	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	116	theme	mitigation	2393:2402	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	4	117	theme	concentration	1080:1092	arg1	gradient					1094:1101	the CO2 concentration gradient	1072:1101	the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	1072:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	1	118	dep	BACKGROUND	80:89	arg1	impacting					159:167	impacting	159:167	is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes	156:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	3	119	with	conductance	667:677	arg1	processes					706:714	C and N processes	698:714	C and N processes in grassland	698:727	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	2	120	theme	water	437:441	arg1	loss					443:446	water loss	437:446	water loss by transpiration	437:463	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	6	121	from	Grass	1590:1594	arg1	reconstruction					1535:1548	historical reconstruction	1524:1548	historical reconstruction of stomatal conductance changes at Park Grass	1524:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	6	121	from	Grass	1590:1594	arg1	changes					1574:1580	stomatal conductance changes	1553:1580	stomatal conductance changes at Park Grass	1553:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	0	122	from	response	37:44	arg1	century					71:77	the last century	62:77	the last century	62:77	Stomatal conductance limited the CO2 response of grassland in the last century.					
33757496	4	123	theme	yields	1062:1067	arg1	ratio					1053:1057	the ratio	1049:1057	the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	1049:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	2	124	theme	water	374:378	arg1	efficiency					384:393	vegetation water use efficiency	363:393	vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration)	363:464	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	125	theme	coupled	314:320	arg1	cycles					322:327	these coupled cycles	308:327	these coupled cycles	308:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	4	126	dep	RESULTS	730:736	arg1	assessed					747:754	assessed	747:754	assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	747:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	11	127	theme	legumes	2539:2545	arg1	expansion					2452:2460	the expansion	2448:2460	the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes	2448:2545	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	3	128	from	processes	706:714	arg1	grassland					719:727	grassland	719:727	grassland	719:727	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	4	129	theme	internal	1139:1146	arg1	space					1152:1156	the leaf internal gas space	1130:1156	the leaf internal gas space	1130:1156	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	2	130	theme	physiological	338:350	arg1	it					330:331	it	330:331	it	330:331	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	130	theme	physiological	338:350	arg1	control					352:358	a physiological control	336:358	a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration)	336:464	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	1	131	theme	terrestrial	235:245	arg1	biomes					247:252	other terrestrial biomes	229:252	other terrestrial biomes	229:252	BACKGROUND The anthropogenic increase of atmospheric CO2 concentration (ca) is impacting carbon (C), water, and nitrogen (N) cycles in grassland and other terrestrial biomes.					
33757496	5	132	dep	correlated	1185:1194	arg1	R2 = 0.70					1205:1213	R2 = 0.70	1205:1213	R2 = 0.70	1205:1213	The two proxies correlated closely (R2 = 0.70), in agreement with the hypothesis.					
33757496	9	133	theme	long-term	1995:2003	arg1	reductions					2005:2014	long-term reductions	1995:2014	long-term reductions of stomatal conductance	1995:2038	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	0	134	theme	grassland	49:57	arg1	response					37:44	the CO2 response	29:44	the CO2 response of grassland in the last century	29:77	Stomatal conductance limited the CO2 response of grassland in the last century.					
33757496	6	135	theme	Park	1585:1588	arg1	Grass					1590:1594	Park Grass	1585:1594	Park Grass	1585:1594	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	2	136	theme	efficiency	384:393	arg1	player					298:303	a key player	292:303	a key player in these coupled cycles	292:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	136	theme	efficiency	384:393	arg1	it					330:331	it	330:331	it	330:331	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	136	theme	efficiency	384:393	arg1	conductance					277:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	136	theme	efficiency	384:393	arg1	control					352:358	a physiological control	336:358	a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration)	336:464	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	6	137	theme	stomatal	1318:1325	arg1	changes					1339:1345	estimated stomatal conductance changes	1308:1345	estimated stomatal conductance changes	1308:1345	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33757496	11	138	theme	N	2334:2334	arg1	supply					2347:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply	2323:2352	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	11	138	theme	N	2334:2334	arg1	strategy					2404:2411	an effective climate change mitigation strategy	2365:2411	an effective climate change mitigation strategy in many grasslands	2365:2430	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	4	139	theme	wide	948:951	arg1	range					953:957	a wide range	946:957	a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci)	946:1166	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	11	140	theme	more	2500:2503	arg1	CO2					2505:2507	the more CO2	2496:2507	the more CO2	2496:2507	Increasing N fertilizer supply may not be an effective climate change mitigation strategy in many grasslands, as it promotes the expansion of grasses at the disadvantage of the more CO2 responsive forbs and N-fixing legumes.					
33757496	9	141	theme	conductance	2028:2038	arg1	reductions					2005:2014	long-term reductions	1995:2014	long-term reductions of stomatal conductance	1995:2038	Although all plots were nitrogen-limited or phosphorus- and nitrogen-co-limited to different degrees, long-term reductions of stomatal conductance were largely independent of fertilizer regimes and soil pH, except for nitrogen fertilizer supply which promoted the abundance of grasses.					
33757496	2	142	theme	stomatal	268:275	arg1	player					298:303	a key player	292:303	a key player in these coupled cycles	292:327	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	2	142	theme	stomatal	268:275	arg1	conductance					277:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance	255:287	Plant canopy stomatal conductance is a key player in these coupled cycles: it is a physiological control of vegetation water use efficiency (the ratio of C gain by photosynthesis to water loss by transpiration), and it responds to photosynthetic activity, which is influenced by vegetation N status.					
33757496	4	143	from	communities	972:982	arg1	Experiment					1002:1011	the Park Grass Experiment	987:1011	the Park Grass Experiment	987:1011	RESULTS Here, we assessed two independent proxies of (growing season-integrating canopy-scale) stomatal conductance changes over the last century: trends of δ18O in cellulose (δ18Ocellulose) in archived herbage from a wide range of grassland communities on the Park Grass Experiment at Rothamsted (U.K.) and changes of the ratio of yields to the CO2 concentration gradient between the atmosphere and the leaf internal gas space (ca - ci).					
33757496	3	144	theme	climate	592:598	arg1	change					600:605	the ca-increase and climate change	572:605	the ca-increase and climate change over the last century	572:627	It is unknown if the ca-increase and climate change over the last century have already affected canopy stomatal conductance and its links with C and N processes in grassland.					
33757496	6	145	theme	δ18Ocellulose	1283:1295	arg1	changes					1297:1303	δ18Ocellulose changes	1283:1303	δ18Ocellulose changes	1283:1303	In addition, the sensitivity of δ18Ocellulose changes to estimated stomatal conductance changes agreed broadly with published sensitivities across a range of contemporary field and controlled environment studies, further supporting the utility of δ18Ocellulose changes for historical reconstruction of stomatal conductance changes at Park Grass.					
33183609	5	0	theme	aqueous	804:810	arg1	solution					812:819	aqueous solution	804:819	aqueous solution	804:819	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	7	1	theme	practical	1187:1195	arg1	wastewater					1197:1206	practical wastewater	1187:1206	practical wastewater	1187:1206	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	4	2	theme	different	597:605	arg1	parameters					618:627	different adsorption parameters	597:627	different adsorption parameters	597:627	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	0	3	theme	rapid	100:104	arg1	separation					106:115	rapid separation	100:115	rapid separation	100:115	Magnetic NiFe2O4/MWCNTs functionalized cellulose bioadsorbent with enhanced adsorption property and rapid separation.					
33183609	7	4	theme	wastewater	1197:1206	arg1	purification					1157:1168	purification	1157:1168	purification	1157:1168	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	4	theme	wastewater	1197:1206	arg1	treatment					1174:1182	treatment	1174:1182	treatment	1174:1182	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	5	theme	m-NiFe2O4/MWCNTs	1007:1022	arg1	cellulose					1024:1032	The m-NiFe2O4/MWCNTs@cellulose	1003:1032	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity	1003:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	5	theme	m-NiFe2O4/MWCNTs	1007:1022	arg1	biomaterials					1140:1151	a promising and recyclable engineering biomaterials	1101:1151	a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater	1101:1206	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	5	6	theme	high	767:770	arg1	capacity					783:790	high adsorption capacity	767:790	high adsorption capacity for CR from aqueous solution	767:819	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	6	7	theme	adsorption	959:968	arg1	capacity					970:977	a maximum adsorption capacity	949:977	a maximum adsorption capacity of 95.70 mg g-1 for CR	949:1000	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	7	8	theme	@	1023:1023	arg1	cellulose					1024:1032	The m-NiFe2O4/MWCNTs@cellulose	1003:1032	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity	1003:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	8	theme	@	1023:1023	arg1	biomaterials					1140:1151	a promising and recyclable engineering biomaterials	1101:1151	a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater	1101:1206	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	3	9	theme	adsorption	550:559	arg1	capacity					561:568	the adsorption capacity	546:568	the adsorption capacity	546:568	Batch experiments were carried out to investigate the adsorption capacity and mechanisms.					
33183609	4	10	theme	parameters	618:627	arg1	temperature					684:694	temperature	684:694	temperature	684:694	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	4	10	theme	parameters	618:627	arg1	concentration					648:660	initial CR concentration	637:660	initial CR concentration	637:660	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	4	10	theme	parameters	618:627	arg1	dosage					673:678	adsorbent dosage	663:678	adsorbent dosage	663:678	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	4	10	theme	parameters	618:627	arg1	Effects					586:592	Effects	586:592	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature	586:694	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	5	11	theme	adsorption	772:781	arg1	capacity					783:790	high adsorption capacity	767:790	high adsorption capacity for CR from aqueous solution	767:819	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	6	12	theme	maximum	951:957	arg1	capacity					970:977	a maximum adsorption capacity	949:977	a maximum adsorption capacity of 95.70 mg g-1 for CR	949:1000	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	4	13	theme	adsorption	607:616	arg1	parameters					618:627	different adsorption parameters	597:627	different adsorption parameters	597:627	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	3	14	theme	Batch	496:500	arg1	experiments					502:512	Batch experiments	496:512	Batch experiments	496:512	Batch experiments were carried out to investigate the adsorption capacity and mechanisms.					
33183609	1	15	theme	m-NiFe2O4/MWCNTs	219:234	arg1	cellulose					236:244	m-NiFe2O4/MWCNTs@cellulose	219:244	m-NiFe2O4/MWCNTs@cellulose	219:244	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	15	theme	m-NiFe2O4/MWCNTs	219:234	arg1	nanoparticles					135:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	6	16	theme	experimental	835:846	arg1	data					848:851	The obtained experimental data	822:851	The obtained experimental data	822:851	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	2	17	theme	cellulose	429:437	arg1	properties					385:394	The chemical and physical properties	359:394	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose	359:437	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose were characterized by XRD, TGA, FT-IR, VSM, SEM and TEM.					
33183609	6	18	theme	obtained	826:833	arg1	data					848:851	The obtained experimental data	822:851	The obtained experimental data	822:851	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	2	19	theme	@	428:428	arg1	cellulose					429:437	the prepared m-NiFe2O4/MWCNTs@cellulose	399:437	the prepared m-NiFe2O4/MWCNTs@cellulose	399:437	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose were characterized by XRD, TGA, FT-IR, VSM, SEM and TEM.					
33183609	0	20	theme	Magnetic	0:7	arg1	NiFe2O4/MWCNTs					9:22	Magnetic NiFe2O4/MWCNTs	0:22	Magnetic NiFe2O4/MWCNTs	0:22	Magnetic NiFe2O4/MWCNTs functionalized cellulose bioadsorbent with enhanced adsorption property and rapid separation.					
33183609	5	21	contain	had	763:765	arg1	cellulose					753:761	m-NiFe2O4/MWCNTs@cellulose	736:761	m-NiFe2O4/MWCNTs@cellulose	736:761	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	5	21	contain	had	763:765	arg2	capacity					783:790	high adsorption capacity	767:790	high adsorption capacity for CR from aqueous solution	767:819	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	1	22	theme	Magnetic	118:125	arg1	cellulose					236:244	m-NiFe2O4/MWCNTs@cellulose	219:244	m-NiFe2O4/MWCNTs@cellulose	219:244	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	22	theme	Magnetic	118:125	arg1	nanoparticles					135:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	2	23	theme	m-NiFe2O4/MWCNTs	412:427	arg1	cellulose					429:437	the prepared m-NiFe2O4/MWCNTs@cellulose	399:437	the prepared m-NiFe2O4/MWCNTs@cellulose	399:437	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose were characterized by XRD, TGA, FT-IR, VSM, SEM and TEM.					
33183609	0	24	theme	cellulose	39:47	arg1	bioadsorbent					49:60	cellulose bioadsorbent	39:60	cellulose bioadsorbent	39:60	Magnetic NiFe2O4/MWCNTs functionalized cellulose bioadsorbent with enhanced adsorption property and rapid separation.					
33183609	5	25	theme	m-NiFe2O4/MWCNTs	736:751	arg1	cellulose					753:761	m-NiFe2O4/MWCNTs@cellulose	736:761	m-NiFe2O4/MWCNTs@cellulose	736:761	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	1	26	theme	NiFe2O4	127:133	arg1	cellulose					236:244	m-NiFe2O4/MWCNTs@cellulose	219:244	m-NiFe2O4/MWCNTs@cellulose	219:244	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	26	theme	NiFe2O4	127:133	arg1	nanoparticles					135:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	5	27	theme	@	752:752	arg1	cellulose					753:761	m-NiFe2O4/MWCNTs@cellulose	736:761	m-NiFe2O4/MWCNTs@cellulose	736:761	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	6	28	theme	isotherm	929:936	arg1	model					938:942	the Langmuir isotherm model	916:942	the Langmuir isotherm model	916:942	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	2	29	theme	prepared	403:410	arg1	cellulose					429:437	the prepared m-NiFe2O4/MWCNTs@cellulose	399:437	the prepared m-NiFe2O4/MWCNTs@cellulose	399:437	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose were characterized by XRD, TGA, FT-IR, VSM, SEM and TEM.					
33183609	7	30	theme	promising	1103:1111	arg1	biomaterials					1140:1151	a promising and recyclable engineering biomaterials	1101:1151	a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater	1101:1206	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	30	theme	promising	1103:1111	arg1	cellulose					1024:1032	The m-NiFe2O4/MWCNTs@cellulose	1003:1032	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity	1003:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	6	31	theme	Langmuir	920:927	arg1	model					938:942	the Langmuir isotherm model	916:942	the Langmuir isotherm model	916:942	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	1	32	theme	magnetic	252:259	arg1	bioadsorbent					261:272	a magnetic bioadsorbent	250:272	a magnetic bioadsorbent	250:272	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	7	33	theme	high	1069:1072	arg1	capacity					1085:1092	high adsorption capacity	1069:1092	high adsorption capacity	1069:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	4	34	theme	adsorbent	663:671	arg1	dosage					673:678	adsorbent dosage	663:678	adsorbent dosage	663:678	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	0	35	theme	adsorption	76:85	arg1	property					87:94	enhanced adsorption property	67:94	enhanced adsorption property	67:94	Magnetic NiFe2O4/MWCNTs functionalized cellulose bioadsorbent with enhanced adsorption property and rapid separation.					
33183609	1	36	theme	aqueous	341:347	arg1	solution					349:356	aqueous solution	341:356	aqueous solution	341:356	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	7	37	theme	adsorption	1074:1083	arg1	capacity					1085:1092	high adsorption capacity	1069:1092	high adsorption capacity	1069:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	2	38	theme	physical	376:383	arg1	properties					385:394	The chemical and physical properties	359:394	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose	359:437	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose were characterized by XRD, TGA, FT-IR, VSM, SEM and TEM.					
33183609	0	39	theme	enhanced	67:74	arg1	property					87:94	enhanced adsorption property	67:94	enhanced adsorption property	67:94	Magnetic NiFe2O4/MWCNTs functionalized cellulose bioadsorbent with enhanced adsorption property and rapid separation.					
33183609	6	40	theme	g-1	991:993	arg1	capacity					970:977	a maximum adsorption capacity	949:977	a maximum adsorption capacity of 95.70 mg g-1 for CR	949:1000	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	7	41	theme	recyclable	1117:1126	arg1	biomaterials					1140:1151	a promising and recyclable engineering biomaterials	1101:1151	a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater	1101:1206	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	41	theme	recyclable	1117:1126	arg1	cellulose					1024:1032	The m-NiFe2O4/MWCNTs@cellulose	1003:1032	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity	1003:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	1	42	used	used	291:294	arg2	nanoparticles					135:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	42	used	used	291:294	arg2	cellulose					236:244	m-NiFe2O4/MWCNTs@cellulose	219:244	m-NiFe2O4/MWCNTs@cellulose	219:244	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	43	theme	multi-walled	153:164	arg1	nanotubes					173:181	multi-walled carbon nanotubes	153:181	multi-walled carbon nanotubes	153:181	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	6	44	theme	mg	988:989	arg1	g-1					991:993	95.70 mg g-1	982:993	95.70 mg g-1 for CR	982:1000	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	5	45	from	solution	812:819	arg1	capacity					783:790	high adsorption capacity	767:790	high adsorption capacity for CR from aqueous solution	767:819	Results demonstrated that m-NiFe2O4/MWCNTs@cellulose had high adsorption capacity for CR from aqueous solution.					
33183609	1	46	theme	carbon	166:171	arg1	nanotubes					173:181	multi-walled carbon nanotubes	153:181	multi-walled carbon nanotubes	153:181	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	7	47	with	cellulose	1024:1032	arg1	capacity					1085:1092	high adsorption capacity	1069:1092	high adsorption capacity	1069:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	47	with	cellulose	1024:1032	arg1	separation					1054:1063	rapid magnetic separation	1039:1063	rapid magnetic separation	1039:1063	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	48	theme	engineering	1128:1138	arg1	biomaterials					1140:1151	a promising and recyclable engineering biomaterials	1101:1151	a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater	1101:1206	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	7	48	theme	engineering	1128:1138	arg1	cellulose					1024:1032	The m-NiFe2O4/MWCNTs@cellulose	1003:1032	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity	1003:1092	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	6	49	theme	pseudo-second-order	874:892	arg1	equation					894:901	the pseudo-second-order equation	870:901	the pseudo-second-order equation	870:901	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	7	50	theme	rapid	1039:1043	arg1	separation					1054:1063	rapid magnetic separation	1039:1063	rapid magnetic separation	1039:1063	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	1	51	theme	cellulose	198:206	arg1	composite					208:216	functionalized cellulose composite	183:216	functionalized cellulose composite	183:216	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	2	52	theme	chemical	363:370	arg1	properties					385:394	The chemical and physical properties	359:394	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose	359:437	The chemical and physical properties of the prepared m-NiFe2O4/MWCNTs@cellulose were characterized by XRD, TGA, FT-IR, VSM, SEM and TEM.					
33183609	7	53	theme	magnetic	1045:1052	arg1	separation					1054:1063	rapid magnetic separation	1039:1063	rapid magnetic separation	1039:1063	The m-NiFe2O4/MWCNTs@cellulose with rapid magnetic separation and high adsorption capacity can be a promising and recyclable engineering biomaterials for purification and treatment of practical wastewater.					
33183609	1	54	theme	functionalized	183:196	arg1	composite					208:216	functionalized cellulose composite	183:216	functionalized cellulose composite	183:216	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	55	theme	@	235:235	arg1	cellulose					236:244	m-NiFe2O4/MWCNTs@cellulose	219:244	m-NiFe2O4/MWCNTs@cellulose	219:244	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	1	55	theme	@	235:235	arg1	nanoparticles					135:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles	118:147	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	4	56	theme	CR	645:646	arg1	concentration					648:660	initial CR concentration	637:660	initial CR concentration	637:660	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
33183609	6	57	theme	95.70	982:986	arg1	mg					988:989	mg	988:989	mg	988:989	The obtained experimental data fitted well with the pseudo-second-order equation and followed the Langmuir isotherm model with a maximum adsorption capacity of 95.70 mg g-1 for CR.					
33183609	1	58	dep	nanoparticles	135:147	arg1	composite					208:216	functionalized cellulose composite	183:216	functionalized cellulose composite	183:216	Magnetic NiFe2O4 nanoparticles and multi-walled carbon nanotubes functionalized cellulose composite (m-NiFe2O4/MWCNTs@cellulose) as a magnetic bioadsorbent was prepared and used for effectively removing Congo Red (CR) from aqueous solution.					
33183609	4	59	theme	initial	637:643	arg1	concentration					648:660	initial CR concentration	637:660	initial CR concentration	637:660	Effects of different adsorption parameters such as initial CR concentration, adsorbent dosage and temperature were studied.					
31953173	5	0	theme	swelling	872:879	arg1	capacity					881:888	higher porosity and swelling capacity	852:888	higher porosity and swelling capacity	852:888	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	6	1	theme	hydrogels	1051:1059	arg1	behavior					1039:1046	longer time swelling behavior	1018:1046	longer time swelling behavior of hydrogels	1018:1059	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	7	2	theme	SBF	1221:1223	arg1	solution					1225:1232	the SBF solution	1217:1232	the SBF solution	1217:1232	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	7	3	theme	apatite	1142:1148	arg1	layer					1150:1154	apatite layer	1142:1154	apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1142:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	9	4	theme	dextran/β-TCP	1398:1410	arg1	hydrogels					1426:1434	dextran/β-TCP nanocomposite hydrogels	1398:1434	dextran/β-TCP nanocomposite hydrogels	1398:1434	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	9	4	theme	dextran/β-TCP	1398:1410	arg1	scaffolds					1449:1457	promising scaffolds	1439:1457	promising scaffolds for bone regeneration applications	1439:1492	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	9	5	theme	nanocomposite	1412:1424	arg1	hydrogels					1426:1434	dextran/β-TCP nanocomposite hydrogels	1398:1434	dextran/β-TCP nanocomposite hydrogels	1398:1434	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	9	5	theme	nanocomposite	1412:1424	arg1	scaffolds					1449:1457	promising scaffolds	1439:1457	promising scaffolds for bone regeneration applications	1439:1492	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	8	6	theme	hybrid	1332:1337	arg1	hydrogels					1339:1347	the developed hybrid hydrogels	1318:1347	the developed hybrid hydrogels	1318:1347	Cell culture assays confirmed biocompatibility of the developed hybrid hydrogels in vitro.					
31953173	4	7	theme	water	663:667	arg1	uptake					669:674	equilibrium water uptake	651:674	equilibrium water uptake	651:674	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	7	8	from	surface	1163:1169	arg1	formation					1129:1137	the formation	1125:1137	the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1125:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	2	9	theme	nanocrystalline	335:349	arg1	particles					382:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	2	10	theme	β-tricalcium	351:362	arg1	particles					382:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	4	11	theme	swelling	680:687	arg1	kinetics					689:696	swelling kinetics	680:696	swelling kinetics	680:696	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	9	12	theme	bone	1463:1466	arg1	applications					1481:1492	bone regeneration applications	1463:1492	bone regeneration applications	1463:1492	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	6	13	theme	second	1073:1078	arg1	kinetics					1086:1093	the second order kinetics	1069:1093	the second order kinetics	1069:1093	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	8	14	theme	culture	1273:1279	arg1	assays					1281:1286	Cell culture assays	1268:1286	Cell culture assays	1268:1286	Cell culture assays confirmed biocompatibility of the developed hybrid hydrogels in vitro.					
31953173	9	15	theme	promising	1439:1447	arg1	hydrogels					1426:1434	dextran/β-TCP nanocomposite hydrogels	1398:1434	dextran/β-TCP nanocomposite hydrogels	1398:1434	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	9	15	theme	promising	1439:1447	arg1	scaffolds					1449:1457	promising scaffolds	1439:1457	promising scaffolds for bone regeneration applications	1439:1492	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	3	16	theme	dextran	496:502	arg1	networks					504:511	the dextran networks	492:511	the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy	492:617	β-TCP was successfully introduced within the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy.					
31953173	5	17	theme	higher	852:857	arg1	capacity					881:888	higher porosity and swelling capacity	852:888	higher porosity and swelling capacity	852:888	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	8	18	theme	developed	1322:1330	arg1	hydrogels					1339:1347	the developed hybrid hydrogels	1318:1347	the developed hybrid hydrogels	1318:1347	Cell culture assays confirmed biocompatibility of the developed hybrid hydrogels in vitro.					
31953173	1	19	theme	key	221:223	arg1	area					225:228	a key area	219:228	a key area of research in tissue engineering	219:262	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	7	20	theme	nanocomposite	1174:1186	arg1	hydrogels					1188:1196	nanocomposite hydrogels	1174:1196	nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1174:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	6	21	theme	depth	894:898	arg1	swelling					900:907	depth swelling	894:907	depth swelling	894:907	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	2	22	theme	β-TCP	375:379	arg1	particles					382:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	2	23	theme	tissue	431:436	arg1	engineering					438:448	bone tissue engineering	426:448	bone tissue engineering	426:448	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	4	24	theme	content	640:646	arg1	effect					624:629	The effect	620:629	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels	620:719	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	7	25	theme	hydrogels	1188:1196	arg1	surface					1163:1169	the surface	1159:1169	the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1159:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	1	26	theme	bioactive	127:135	arg1	scaffolds					155:163	bioactive three-dimensional scaffolds	127:163	bioactive three-dimensional scaffolds	127:163	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	2	27	theme	bone	426:429	arg1	engineering					438:448	bone tissue engineering	426:448	bone tissue engineering	426:448	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	4	28	theme	β-TCP	634:638	arg1	content					640:646	β-TCP content	634:646	β-TCP content	634:646	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	5	29	theme	nanoparticles	795:807	arg1	distribution					773:784	the homogeneous distribution	757:784	the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix	757:835	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	2	30	theme	phosphate	364:372	arg1	particles					382:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	nanocrystalline β-tricalcium phosphate (β-TCP) particles	335:390	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	1	31	theme	research	233:240	arg1	area					225:228	a key area	219:228	a key area of research in tissue engineering	219:262	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	7	32	from	layer	1150:1154	arg1	surface					1163:1169	the surface	1159:1169	the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1159:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	4	33	theme	equilibrium	651:661	arg1	uptake					669:674	equilibrium water uptake	651:674	equilibrium water uptake	651:674	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	7	34	theme	XRD	1096:1098	arg1	measurements					1100:1111	XRD measurements	1096:1111	XRD measurements	1096:1111	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	6	35	theme	water	974:978	arg1	diffusion					980:988	water diffusion	974:988	water diffusion	974:988	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	1	36	theme	scaffolds	155:163	arg1	Design					117:122	Design	117:122	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration	117:210	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	3	37	theme	ionic	548:552	arg1	interactions					554:565	intermolecular ionic interactions	533:565	intermolecular ionic interactions	533:565	β-TCP was successfully introduced within the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy.					
31953173	0	38	theme	β-tricalcium	60:71	arg1	phosphate					73:81	dextran/nanocrystalline β-tricalcium phosphate	36:81	dextran/nanocrystalline β-tricalcium phosphate	36:81	Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds.					
31953173	7	39	from	immersion	1204:1212	arg1	solution					1225:1232	the SBF solution	1217:1232	the SBF solution	1217:1232	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	2	40	theme	porous	273:278	arg1	hydrogels					287:295	porous hybrid hydrogels	273:295	porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles	273:390	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	1	41	theme	tissue	245:250	arg1	engineering					252:262	tissue engineering	245:262	tissue engineering	245:262	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	0	42	theme	dextran/nanocrystalline	36:58	arg1	phosphate					73:81	dextran/nanocrystalline β-tricalcium phosphate	36:81	dextran/nanocrystalline β-tricalcium phosphate	36:81	Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds.					
31953173	5	43	theme	hydrogel	821:828	arg1	matrix					830:835	the hydrogel matrix	817:835	the hydrogel matrix	817:835	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	4	44	from	effect	624:629	arg1	uptake					669:674	equilibrium water uptake	651:674	equilibrium water uptake	651:674	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	4	44	from	effect	624:629	arg1	kinetics					689:696	swelling kinetics	680:696	swelling kinetics	680:696	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	6	45	theme	swelling	964:971	arg1	stage					955:959	the early stage	945:959	the early stage of swelling	945:971	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	6	46	theme	longer	1018:1023	arg1	behavior					1039:1046	longer time swelling behavior	1018:1046	longer time swelling behavior of hydrogels	1018:1059	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	8	47	theme	hydrogels	1339:1347	arg1	biocompatibility					1298:1313	biocompatibility	1298:1313	biocompatibility of the developed hybrid hydrogels	1298:1347	Cell culture assays confirmed biocompatibility of the developed hybrid hydrogels in vitro.					
31953173	6	48	theme	swelling	1030:1037	arg1	behavior					1039:1046	longer time swelling behavior	1018:1046	longer time swelling behavior of hydrogels	1018:1059	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	5	49	theme	homogeneous	761:771	arg1	distribution					773:784	the homogeneous distribution	757:784	the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix	757:835	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	9	50	theme	regeneration	1468:1479	arg1	applications					1481:1492	bone regeneration applications	1463:1492	bone regeneration applications	1463:1492	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	1	51	from	engineering	252:262	arg1	area					225:228	a key area	219:228	a key area of research in tissue engineering	219:262	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	3	52	theme	hydrogen	571:578	arg1	bonding					580:586	hydrogen bonding	571:586	hydrogen bonding confirmed by FTIR spectroscopy	571:617	β-TCP was successfully introduced within the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy.					
31953173	0	53	theme	phosphate	73:81	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds.					
31953173	0	53	theme	phosphate	73:81	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds.					
31953173	6	54	theme	time	1025:1028	arg1	behavior					1039:1046	longer time swelling behavior	1018:1046	longer time swelling behavior of hydrogels	1018:1059	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	7	55	theme	layer	1150:1154	arg1	formation					1129:1137	the formation	1125:1137	the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1125:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	9	56	theme	obtained	1363:1370	arg1	results					1372:1378	The obtained results	1359:1378	The obtained results	1359:1378	The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.					
31953173	1	57	from	research	233:240	arg1	engineering					252:262	tissue engineering	245:262	tissue engineering	245:262	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	1	58	dep	Design	117:122	arg1	support					168:174	support	168:174	to support bone tissue repair and regeneration	165:210	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	2	59	dep	tailor	397:402	arg1	made					404:407	made	404:407	were tailor made as scaffolds for bone tissue engineering	392:448	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	6	60	theme	early	949:953	arg1	stage					955:959	the early stage	945:959	the early stage of swelling	945:971	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	1	61	theme	bone	176:179	arg1	repair					188:193	bone tissue repair	176:193	bone tissue repair	176:193	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	1	62	theme	three-dimensional	137:153	arg1	scaffolds					155:163	bioactive three-dimensional scaffolds	127:163	bioactive three-dimensional scaffolds	127:163	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	6	63	theme	order	1080:1084	arg1	kinetics					1086:1093	the second order kinetics	1069:1093	the second order kinetics	1069:1093	In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics.					
31953173	5	64	theme	β-TCP	789:793	arg1	nanoparticles					795:807	β-TCP nanoparticles	789:807	β-TCP nanoparticles	789:807	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	8	65	theme	Cell	1268:1271	arg1	assays					1281:1286	Cell culture assays	1268:1286	Cell culture assays	1268:1286	Cell culture assays confirmed biocompatibility of the developed hybrid hydrogels in vitro.					
31953173	1	66	from	area	225:228	arg1	engineering					252:262	tissue engineering	245:262	tissue engineering	245:262	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	1	67	theme	tissue	181:186	arg1	repair					188:193	bone tissue repair	176:193	bone tissue repair	176:193	Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering.					
31953173	5	68	theme	porosity	859:866	arg1	capacity					881:888	higher porosity and swelling capacity	852:888	higher porosity and swelling capacity	852:888	It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity.					
31953173	3	69	theme	intermolecular	533:546	arg1	interactions					554:565	intermolecular ionic interactions	533:565	intermolecular ionic interactions	533:565	β-TCP was successfully introduced within the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy.					
31953173	3	70	theme	FTIR	601:604	arg1	spectroscopy					606:617	FTIR spectroscopy	601:617	FTIR spectroscopy	601:617	β-TCP was successfully introduced within the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy.					
31953173	4	71	theme	hydrogels	711:719	arg1	uptake					669:674	equilibrium water uptake	651:674	equilibrium water uptake	651:674	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	4	71	theme	hydrogels	711:719	arg1	kinetics					689:696	swelling kinetics	680:696	swelling kinetics	680:696	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
31953173	0	72	theme	hydrogel	97:104	arg1	scaffolds					106:114	hydrogel scaffolds	97:114	hydrogel scaffolds	97:114	Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds.					
31953173	2	73	theme	hybrid	280:285	arg1	hydrogels					287:295	porous hybrid hydrogels	273:295	porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles	273:390	Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering.					
31953173	7	74	from	formation	1129:1137	arg1	surface					1163:1169	the surface	1159:1169	the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity	1159:1265	XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity.					
31953173	4	75	theme	composite	701:709	arg1	hydrogels					711:719	composite hydrogels	701:719	composite hydrogels	701:719	The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated.					
33838196	0	0	theme	antimicrobial	98:110	arg1	agents					112:117	green antimicrobial agents	92:117	green antimicrobial agents	92:117	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	8	1	theme	bacteria	1612:1619	arg1	cell					1621:1624	the bacteria cell	1608:1624	the bacteria cell	1608:1624	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	0	2	theme	green	92:96	arg1	agents					112:117	green antimicrobial agents	92:117	green antimicrobial agents	92:117	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	7	3	theme	AgNPs	1111:1115	arg1	number					1101:1106	the number	1097:1106	the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3	1097:1189	The bacterial performance was also controlled by changing the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3.					
33838196	1	4	theme	few-layered	173:183	arg1	graphene					185:192	few-layered graphene	173:192	few-layered graphene utilizing the biowaste of potato peels	173:231	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed.					
33838196	8	5	theme	decorated	1226:1234	arg1	graphene					1236:1243	silver decorated graphene	1219:1243	silver decorated graphene	1219:1243	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	7	6	theme	graphite/AgNO3	1176:1189	arg1	ratios					1166:1171	the mass ratios	1157:1171	the mass ratios of graphite/AgNO3	1157:1189	The bacterial performance was also controlled by changing the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3.					
33838196	6	7	theme	graphene	893:900	arg1	nanocomposites					902:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	8	8	theme	pure	1350:1353	arg1	mm					1376:1377	11.2, 13.5 mm	1365:1377	mm	1376:1377	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	8	8	theme	pure	1350:1353	arg1	graphene					1355:1362	the pure graphene	1346:1362	the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus	1346:1404	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	3	9	theme	graphite	542:549	arg1	structure					551:559	graphite structure	542:559	graphite structure	542:559	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	6	10	theme	decorated	883:891	arg1	nanocomposites					902:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	8	11	theme	composite	1324:1332	arg1	case					1303:1306	the case	1299:1306	the case of MPC-GRP-Ag30 composite	1299:1332	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	9	12	theme	green	1647:1651	arg1	agents					1685:1690	green and biocompatible antibacterial agents	1647:1690	green and biocompatible antibacterial agents	1647:1690	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	3	13	theme	structure	551:559	arg1	weight					532:537	the weight	528:537	the weight of graphite structure	528:559	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	9	14	theme	biowaste	1731:1738	arg1	products					1740:1747	the biowaste products	1727:1747	the biowaste products	1727:1747	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	6	15	theme	silver	876:881	arg1	nanocomposites					902:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	5	16	dep	morphology	777:786	arg1	the					773:775	the	773:775	the	773:775	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	4	17	theme	silver	608:613	arg1	nanoparticles					615:627	silver nanoparticles	608:627	silver nanoparticles	608:627	The graphene sheets were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites.					
33838196	3	18	theme	%	517:517	arg1	quality					504:510	quality	504:510	quality of 50%	504:517	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	3	18	theme	%	517:517	arg1	diameter					491:498	a high size diameter	479:498	a high size diameter	479:498	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	1	19	theme	graphene	185:192	arg1	preparation					158:168	the preparation	154:168	the preparation of few-layered graphene utilizing the biowaste of potato peels	154:231	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed.					
33838196	4	20	theme	graphene	566:573	arg1	sheets					575:580	The graphene sheets	562:580	The graphene sheets	562:580	The graphene sheets were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites.					
33838196	5	21	theme	size	792:795	arg1	diameter					797:804	size diameter	792:804	size diameter	792:804	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	8	22	theme	sheets	1463:1468	arg1	edge					1446:1449	the blade edge	1436:1449	the blade edge of graphene sheets	1436:1468	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	7	23	theme	mass	1161:1164	arg1	ratios					1166:1171	the mass ratios	1157:1171	the mass ratios of graphite/AgNO3	1157:1189	The bacterial performance was also controlled by changing the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3.					
33838196	8	24	theme	silver	1219:1224	arg1	graphene					1236:1243	silver decorated graphene	1219:1243	silver decorated graphene	1219:1243	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	0	25	theme	phosphonic	12:21	arg1	acid					23:26	N-methylene phosphonic acid	0:26	N-methylene phosphonic acid chitosan/graphene sheets	0:51	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	2	26	from	exfoliated	350:359	arg1	MPC					402:404	MPC	402:404	MPC	402:404	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	26	from	exfoliated	350:359	arg1	chitosan					392:399	N-methylene phosphonic acid chitosan	364:399	N-methylene phosphonic acid chitosan (MPC)	364:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	27	theme	graphite	317:324	arg1	structure					326:334	a new graphite structure	311:334	a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC)	311:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	27	theme	graphite	317:324	arg1	exfoliated					350:359	exfoliated	350:359	exfoliated	350:359	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	8	28	theme	graphene	1454:1461	arg1	sheets					1463:1468	graphene sheets	1454:1468	graphene sheets	1454:1468	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	4	29	theme	new	661:663	arg1	nanocomposites					665:678	new nanocomposites	661:678	new nanocomposites	661:678	The graphene sheets were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites.					
33838196	0	30	theme	N-methylene	0:10	arg1	acid					23:26	N-methylene phosphonic acid	0:26	N-methylene phosphonic acid chitosan/graphene sheets	0:51	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	6	31	theme	UV/Vis	960:965	arg1	spectrophotometer					967:983	UV/Vis spectrophotometer	960:983	UV/Vis spectrophotometer	960:983	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	8	32	theme	inhibition	1196:1205	arg1	diameter					1207:1214	The inhibition diameter	1192:1214	The inhibition diameter of silver decorated graphene	1192:1243	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	2	33	theme	new	313:315	arg1	structure					326:334	a new graphite structure	311:334	a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC)	311:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	33	theme	new	313:315	arg1	exfoliated					350:359	exfoliated	350:359	exfoliated	350:359	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	34	theme	acid	387:390	arg1	MPC					402:404	MPC	402:404	MPC	402:404	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	34	theme	acid	387:390	arg1	chitosan					392:399	N-methylene phosphonic acid chitosan	364:399	N-methylene phosphonic acid chitosan (MPC)	364:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	0	35	theme	chitosan/graphene	28:44	arg1	sheets					46:51	N-methylene phosphonic acid chitosan/graphene sheets	0:51	N-methylene phosphonic acid chitosan/graphene sheets	0:51	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	9	36	theme	Such	1627:1630	arg1	findings					1632:1639	Such findings	1627:1639	Such findings	1627:1639	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	6	37	theme	antimicrobial	1017:1029	arg1	agents					1031:1036	antimicrobial agents	1017:1036	antimicrobial agents	1017:1036	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	3	38	theme	sheets	467:472	arg1	formation					445:453	the formation	441:453	the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure	441:559	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	0	39	theme	acid	23:26	arg1	sheets					46:51	N-methylene phosphonic acid chitosan/graphene sheets	0:51	N-methylene phosphonic acid chitosan/graphene sheets	0:51	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	9	40	attach	derived	1714:1720	arg1	products					1740:1747	the biowaste products	1727:1747	the biowaste products	1727:1747	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	9	40	attach	derived	1714:1720	arg2	graphene					1705:1712	the graphene	1701:1712	the graphene derived from the biowaste products	1701:1747	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	6	41	theme	graphene	862:869	arg1	nanocomposites					902:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	1	42	theme	green	122:126	arg1	approach					141:148	A green and scalable approach	120:148	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels	120:231	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed.					
33838196	1	43	theme	potato	220:225	arg1	peels					227:231	potato peels	220:231	potato peels	220:231	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed.					
33838196	7	44	theme	graphene	1132:1139	arg1	sheets					1141:1146	graphene sheets	1132:1146	graphene sheets based on the mass ratios of graphite/AgNO3	1132:1189	The bacterial performance was also controlled by changing the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3.					
33838196	8	45	with	interaction	1566:1576	arg1	parts					1599:1603	the cytoplasmic parts	1583:1603	the cytoplasmic parts of the bacteria cell	1583:1624	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	3	46	with	sheets	467:472	arg1	quality					504:510	quality	504:510	quality of 50%	504:517	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	3	46	with	sheets	467:472	arg1	diameter					491:498	a high size diameter	479:498	a high size diameter	479:498	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	4	47	theme	microwave	635:643	arg1	power					645:649	microwave power	635:649	microwave power	635:649	The graphene sheets were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites.					
33838196	8	48	theme	graphene	1236:1243	arg1	diameter					1207:1214	The inhibition diameter	1192:1214	The inhibition diameter of silver decorated graphene	1192:1243	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	1	49	theme	peels	227:231	arg1	biowaste					208:215	the biowaste	204:215	the biowaste of potato peels	204:231	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed.					
33838196	3	50	theme	exfoliation	412:422	arg1	process					424:430	The exfoliation process	408:430	The exfoliation process	408:430	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	8	51	theme	silver	1516:1521	arg1	cations					1523:1529	release silver cations	1508:1529	release silver cations	1508:1529	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	5	52	theme	graphite	708:715	arg1	nitrate					728:734	the graphite and silver nitrate	704:734	nitrate	728:734	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	6	53	theme	structure	851:859	arg1	nanocomposites					902:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites	831:915	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	2	54	from	chitosan	392:399	arg1	structure					326:334	a new graphite structure	311:334	a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC)	311:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	54	from	chitosan	392:399	arg1	exfoliated					350:359	exfoliated	350:359	exfoliated	350:359	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	3	55	theme	graphene	458:465	arg1	sheets					467:472	graphene sheets	458:472	graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure	458:559	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	0	56	theme	silver	68:73	arg1	nanoparticles					75:87	silver nanoparticles	68:87	silver nanoparticles as green antimicrobial agents	68:117	N-methylene phosphonic acid chitosan/graphene sheets decorated with silver nanoparticles as green antimicrobial agents.					
33838196	8	57	theme	release	1508:1514	arg1	cations					1523:1529	release silver cations	1508:1529	release silver cations	1508:1529	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	9	58	theme	biocompatible	1657:1669	arg1	agents					1685:1690	green and biocompatible antibacterial agents	1647:1690	green and biocompatible antibacterial agents	1647:1690	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	6	59	theme	MPC	847:849	arg1	structure					851:859	The as-prepared MPC structure	831:859	The as-prepared MPC structure	831:859	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	3	60	theme	size	486:489	arg1	diameter					491:498	a high size diameter	479:498	a high size diameter	479:498	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	7	61	theme	bacterial	1043:1051	arg1	performance					1053:1063	The bacterial performance	1039:1063	The bacterial performance	1039:1063	The bacterial performance was also controlled by changing the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3.					
33838196	9	62	theme	antibacterial	1671:1683	arg1	agents					1685:1690	green and biocompatible antibacterial agents	1647:1690	green and biocompatible antibacterial agents	1647:1690	Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.					
33838196	6	63	theme	as-prepared	835:845	arg1	structure					851:859	The as-prepared MPC structure	831:859	The as-prepared MPC structure	831:859	The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents.					
33838196	1	64	theme	scalable	132:139	arg1	approach					141:148	A green and scalable approach	120:148	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels	120:231	A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed.					
33838196	8	65	theme	bacteria	1486:1493	arg1	membrane					1495:1502	the bacteria membrane	1482:1502	the bacteria membrane	1482:1502	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	8	66	theme	cytoplasmic	1587:1597	arg1	parts					1599:1603	the cytoplasmic parts	1583:1603	the cytoplasmic parts of the bacteria cell	1583:1624	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	5	67	theme	mass	685:688	arg1	ratio					690:694	The mass ratio	681:694	The mass ratio between the graphite and silver nitrate	681:734	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	4	68	dep	green	587:591	arg1	decorated					593:601	decorated	593:601	were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites	582:678	The graphene sheets were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites.					
33838196	5	69	theme	silver	809:814	arg1	nanoparticles					816:828	silver nanoparticles	809:828	silver nanoparticles	809:828	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	3	70	theme	high	481:484	arg1	diameter					491:498	a high size diameter	479:498	a high size diameter	479:498	The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure.					
33838196	5	71	theme	nanoparticles	816:828	arg1	morphology					777:786	morphology	777:786	morphology	777:786	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	5	71	theme	nanoparticles	816:828	arg1	diameter					797:804	size diameter	792:804	size diameter	792:804	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
33838196	2	72	theme	phosphonic	376:385	arg1	acid					387:390	N-methylene phosphonic acid	364:390	N-methylene phosphonic acid chitosan (MPC)	364:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	8	73	theme	blade	1440:1444	arg1	edge					1446:1449	the blade edge	1436:1449	the blade edge of graphene sheets	1436:1468	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	2	74	theme	potato	257:262	arg1	peels					264:268	The potato peels	253:268	The potato peels	253:268	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	2	75	theme	N-methylene	364:374	arg1	acid					387:390	N-methylene phosphonic acid	364:390	N-methylene phosphonic acid chitosan (MPC)	364:405	The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC).					
33838196	8	76	theme	cell	1621:1624	arg1	parts					1599:1603	the cytoplasmic parts	1583:1603	the cytoplasmic parts of the bacteria cell	1583:1624	The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell.					
33838196	5	77	theme	silver	721:726	arg1	nitrate					728:734	the graphite and silver nitrate	704:734	nitrate	728:734	The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles.					
32651201	12	0	dep	V.	1654:1655	arg1	cholerae					1657:1664	cholerae	1657:1664	cholerae	1657:1664	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	8	1	theme	species-specific	1084:1099	arg1	CAI-1					1113:1117	the species-specific autoinducer CAI-1	1080:1117	the species-specific autoinducer CAI-1	1080:1117	Through this analysis, we found that signaling via the species-specific autoinducer CAI-1, but not the interspecies autoinducer AI-2, influences chb expression.					
32651201	8	2	theme	chb	1174:1176	arg1	expression					1178:1187	chb expression	1174:1187	chb expression	1174:1187	Through this analysis, we found that signaling via the species-specific autoinducer CAI-1, but not the interspecies autoinducer AI-2, influences chb expression.					
32651201	10	3	theme	Microbial	1388:1396	arg1	species					1398:1404	Microbial species	1388:1404	Microbial species	1388:1404	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	0	4	theme	Species-Specific	0:15	arg1	Sensing					24:30	Species-Specific Quorum Sensing	0:30	Species-Specific Quorum Sensing	0:30	Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.					
32651201	13	5	theme	cholerae-chitin	1874:1888	arg1	interactions					1890:1901	V. cholerae-chitin interactions	1871:1901	V. cholerae-chitin interactions	1871:1901	Here, we show that V. cholerae uses QS to regulate the expression of one locus required for V. cholerae-chitin interactions.					
32651201	2	6	theme	waterborne	335:344	arg1	transmission					346:357	waterborne transmission	335:357	waterborne transmission	335:357	V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera.					
32651201	1	7	theme	crustacean	210:219	arg1	zooplankton					221:231	crustacean zooplankton	210:231	crustacean zooplankton	210:231	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	5	8	theme	screens	692:698	arg1	series					674:679	a series	672:679	a series of genetic screens	672:698	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	9	theme	chb	795:797	arg1	operon					799:804	the chb operon	791:804	the chb operon	791:804	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	8	10	theme	autoinducer	1145:1155	arg1	AI-2					1157:1160	the interspecies autoinducer AI-2	1128:1160	the interspecies autoinducer AI-2	1128:1160	Through this analysis, we found that signaling via the species-specific autoinducer CAI-1, but not the interspecies autoinducer AI-2, influences chb expression.					
32651201	2	11	theme	V.	259:260	arg1	interactions					278:289	V. cholerae-chitin interactions	259:289	V. cholerae-chitin interactions	259:289	V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera.					
32651201	7	12	theme	sensing	944:950	arg1	cascade					952:958	the canonical quorum sensing cascade	923:958	the canonical quorum sensing cascade in V. cholerae	923:973	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	1	13	theme	aquatic	240:246	arg1	reservoir					248:256	its aquatic reservoir	236:256	its aquatic reservoir	236:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	7	14	theme	canonical	927:935	arg1	cascade					952:958	the canonical quorum sensing cascade	923:958	the canonical quorum sensing cascade in V. cholerae	923:973	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	7	15	theme	V.	963:964	arg1	cholerae					966:973	V. cholerae	963:973	V. cholerae	963:973	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	1	16	theme	complex	149:155	arg1	communities					171:181	complex multicellular communities	149:181	complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir	149:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	11	17	located	found	1568:1572	arg2	cholerae					1556:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	11	17	located	found	1568:1572	arg1	communities					1577:1587	communities	1577:1587	communities on the chitinous shells of crustaceans in its aquatic reservoir	1577:1651	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	3	18	from	changes	411:417	arg1	expression					427:436	gene expression	422:436	gene expression in this pathogen	422:453	This is due, in part, to chitin-induced changes in gene expression in this pathogen.					
32651201	5	19	theme	sensing	751:757	arg1	repressor					778:786	a direct repressor	769:786	a direct repressor of the chb operon	769:804	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	19	theme	sensing	751:757	arg1	HapR					760:763	HapR	760:763	HapR	760:763	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	19	theme	sensing	751:757	arg1	regulator					731:739	the master regulator	720:739	the master regulator of quorum sensing	720:757	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	12	20	theme	V.	1654:1655	arg1	interactions					1666:1677	V. cholerae interactions	1654:1677	V. cholerae interactions with chitin	1654:1689	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	12	21	dep	survival	1712:1719	arg1	the					1708:1710	the	1708:1710	the	1708:1710	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	3	22	from	part	387:390	arg1	due					379:381	due	379:381	due	379:381	This is due, in part, to chitin-induced changes in gene expression in this pathogen.					
32651201	5	23	theme	master	724:729	arg1	repressor					778:786	a direct repressor	769:786	a direct repressor of the chb operon	769:804	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	23	theme	master	724:729	arg1	HapR					760:763	HapR	760:763	HapR	760:763	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	23	theme	master	724:729	arg1	regulator					731:739	the master regulator	720:739	the master regulator of quorum sensing	720:757	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	9	24	from	niche.IMPORTANCE	1299:1314	arg1	nature					1319:1324	nature	1319:1324	nature	1319:1324	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	10	25	dep	density	1420:1426	arg1	the					1416:1418	the	1416:1418	the	1416:1418	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	1	26	theme	marine	99:104	arg1	pathogen					118:125	The marine facultative pathogen	95:125	The marine facultative pathogen Vibrio cholerae	95:141	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	4	27	theme	chitobiose	551:560	arg1	chb					582:584	chb	582:584	chb	582:584	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	4	27	theme	chitobiose	551:560	arg1	operon					574:579	the chitobiose utilization operon	547:579	the chitobiose utilization operon (chb)	547:585	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	4	27	theme	chitobiose	551:560	arg1	locus					540:544	one locus	536:544	one locus	536:544	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	10	28	theme	chemical	1471:1478	arg1	cues					1480:1483	chemical cues	1471:1483	chemical cues using a process called quorum sensing (QS)	1471:1526	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	9	29	theme	environmental	1285:1297	arg1	niche.IMPORTANCE					1299:1314	its environmental niche.IMPORTANCE	1281:1314	its environmental niche.IMPORTANCE In nature	1281:1324	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	3	30	theme	gene	422:425	arg1	expression					427:436	gene expression	422:436	gene expression in this pathogen	422:453	This is due, in part, to chitin-induced changes in gene expression in this pathogen.					
32651201	10	31	theme	community	1453:1461	arg1	composition					1432:1442	composition	1432:1442	composition	1432:1442	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	10	31	theme	community	1453:1461	arg1	density					1420:1426	density	1420:1426	density	1420:1426	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	4	32	theme	chitin	643:648	arg1	disaccharide					650:661	the chitin disaccharide	639:661	the chitin disaccharide	639:661	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	12	33	theme	pathogen	1769:1776	arg1	transmission					1748:1759	waterborne transmission	1737:1759	waterborne transmission	1737:1759	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	12	33	theme	pathogen	1769:1776	arg1	evolution					1722:1730	evolution	1722:1730	evolution	1722:1730	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	12	33	theme	pathogen	1769:1776	arg1	survival					1712:1719	survival	1712:1719	survival	1712:1719	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	0	34	theme	Utilization	57:67	arg1	Locus					69:73	the Chitobiose Utilization Locus	42:73	the Chitobiose Utilization Locus in Vibrio cholerae	42:92	Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.					
32651201	9	35	theme	regulation	1226:1235	arg1	phenomenon					1195:1204	This phenomenon	1190:1204	This phenomenon of species-specific regulation	1190:1235	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	4	36	dep	uptake	614:619	arg1	the					610:612	the	610:612	the	610:612	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	11	37	theme	pathogen	1540:1547	arg1	cholerae					1556:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	2	38	theme	cholera	362:368	arg1	evolution					320:328	evolution	320:328	evolution	320:328	V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera.					
32651201	2	38	theme	cholera	362:368	arg1	growth					312:317	growth	312:317	growth	312:317	V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera.					
32651201	2	38	theme	cholera	362:368	arg1	transmission					346:357	waterborne transmission	335:357	waterborne transmission	335:357	V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera.					
32651201	12	39	theme	waterborne	1737:1746	arg1	transmission					1748:1759	waterborne transmission	1737:1759	waterborne transmission	1737:1759	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	10	40	theme	quorum	1508:1513	arg1	sensing					1515:1521	quorum sensing	1508:1521	quorum sensing (QS)	1508:1526	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	10	40	theme	quorum	1508:1513	arg1	QS					1524:1525	QS	1524:1525	QS	1524:1525	Microbial species can sense the density and composition of their community through chemical cues using a process called quorum sensing (QS).					
32651201	6	41	theme	V.	848:849	arg1	cholerae					851:858	V. cholerae	848:858	V. cholerae	848:858	We also found that the levels of HapR in V. cholerae are regulated by the ClpAP protease.					
32651201	6	42	from	levels	830:835	arg1	cholerae					851:858	V. cholerae	848:858	V. cholerae	848:858	We also found that the levels of HapR in V. cholerae are regulated by the ClpAP protease.					
32651201	6	43	theme	HapR	840:843	arg1	levels					830:835	the levels	826:835	the levels of HapR in V. cholerae	826:858	We also found that the levels of HapR in V. cholerae are regulated by the ClpAP protease.					
32651201	1	44	theme	chitinous	190:198	arg1	shells					200:205	the chitinous shells	186:205	the chitinous shells of crustacean zooplankton in its aquatic reservoir	186:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	8	45	theme	autoinducer	1101:1111	arg1	CAI-1					1113:1117	the species-specific autoinducer CAI-1	1080:1117	the species-specific autoinducer CAI-1	1080:1117	Through this analysis, we found that signaling via the species-specific autoinducer CAI-1, but not the interspecies autoinducer AI-2, influences chb expression.					
32651201	11	46	theme	crustaceans	1616:1626	arg1	shells					1606:1611	the chitinous shells	1592:1611	the chitinous shells of crustaceans in its aquatic reservoir	1592:1651	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	7	47	theme	HapR-dependent	1006:1019	arg1	manner					1021:1026	an HapR-dependent manner	1003:1026	an HapR-dependent manner	1003:1026	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	13	48	theme	V.	1871:1872	arg1	interactions					1890:1901	V. cholerae-chitin interactions	1871:1901	V. cholerae-chitin interactions	1871:1901	Here, we show that V. cholerae uses QS to regulate the expression of one locus required for V. cholerae-chitin interactions.					
32651201	4	49	theme	disaccharide	650:661	arg1	catabolism					625:634	catabolism	625:634	catabolism	625:634	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	4	49	theme	disaccharide	650:661	arg1	uptake					614:619	uptake	614:619	uptake	614:619	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	5	50	theme	genetic	684:690	arg1	screens					692:698	genetic screens	684:698	genetic screens	684:698	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	1	51	theme	zooplankton	221:231	arg1	shells					200:205	the chitinous shells	186:205	the chitinous shells of crustacean zooplankton in its aquatic reservoir	186:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	9	52	dep	multicellular	1344:1356	arg1	communities					1375:1385	communities	1375:1385	communities	1375:1385	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	7	53	theme	chb	985:987	arg1	expression					989:998	chb expression	985:998	chb expression	985:998	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	3	54	from	due	379:381	arg1	part					387:390	part	387:390	part	387:390	This is due, in part, to chitin-induced changes in gene expression in this pathogen.					
32651201	2	55	theme	cholerae-chitin	262:276	arg1	interactions					278:289	V. cholerae-chitin interactions	259:289	V. cholerae-chitin interactions	259:289	V. cholerae-chitin interactions are critical for the growth, evolution, and waterborne transmission of cholera.					
32651201	11	56	from	communities	1577:1587	arg1	shells					1606:1611	the chitinous shells	1592:1611	the chitinous shells of crustaceans in its aquatic reservoir	1592:1651	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	5	57	theme	operon	799:804	arg1	repressor					778:786	a direct repressor	769:786	a direct repressor of the chb operon	769:804	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	57	theme	operon	799:804	arg1	regulator					731:739	the master regulator	720:739	the master regulator of quorum sensing	720:757	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	8	58	theme	interspecies	1132:1143	arg1	AI-2					1157:1160	the interspecies autoinducer AI-2	1128:1160	the interspecies autoinducer AI-2	1128:1160	Through this analysis, we found that signaling via the species-specific autoinducer CAI-1, but not the interspecies autoinducer AI-2, influences chb expression.					
32651201	6	59	theme	ClpAP	881:885	arg1	protease					887:894	the ClpAP protease	877:894	the ClpAP protease	877:894	We also found that the levels of HapR in V. cholerae are regulated by the ClpAP protease.					
32651201	7	60	from	cascade	952:958	arg1	cholerae					966:973	V. cholerae	963:973	V. cholerae	963:973	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	7	61	theme	quorum	937:942	arg1	sensing					944:950	quorum sensing	937:950	the canonical quorum sensing cascade in V. cholerae	923:973	Furthermore, we show that the canonical quorum sensing cascade in V. cholerae regulates chb expression in an HapR-dependent manner.					
32651201	13	62	theme	locus	1852:1856	arg1	expression					1834:1843	the expression	1830:1843	the expression of one locus required for V. cholerae-chitin interactions	1830:1901	Here, we show that V. cholerae uses QS to regulate the expression of one locus required for V. cholerae-chitin interactions.					
32651201	0	63	theme	Vibrio	78:83	arg1	cholerae					85:92	Vibrio cholerae	78:92	Vibrio cholerae	78:92	Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.					
32651201	9	64	theme	species-specific	1209:1224	arg1	regulation					1226:1235	species-specific regulation	1209:1235	species-specific regulation	1209:1235	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	1	65	theme	multicellular	157:169	arg1	communities					171:181	complex multicellular communities	149:181	complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir	149:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	4	66	theme	chitin-induced	507:520	arg1	expression					522:531	chitin-induced expression	507:531	chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide	507:661	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	1	67	dep	pathogen	118:125	arg1	cholerae					134:141	Vibrio cholerae	127:141	The marine facultative pathogen Vibrio cholerae	95:141	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	5	68	theme	quorum	744:749	arg1	sensing					751:757	quorum sensing	744:757	quorum sensing	744:757	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	1	69	from	shells	200:205	arg1	reservoir					248:256	its aquatic reservoir	236:256	its aquatic reservoir	236:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	3	70	from	expression	427:436	arg1	pathogen					446:453	this pathogen	441:453	this pathogen	441:453	This is due, in part, to chitin-induced changes in gene expression in this pathogen.					
32651201	0	71	from	Locus	69:73	arg1	cholerae					85:92	Vibrio cholerae	78:92	Vibrio cholerae	78:92	Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.					
32651201	13	72	theme	V.	1798:1799	arg1	cholerae					1801:1808	V. cholerae	1798:1808	V. cholerae	1798:1808	Here, we show that V. cholerae uses QS to regulate the expression of one locus required for V. cholerae-chitin interactions.					
32651201	1	73	from	communities	171:181	arg1	shells					200:205	the chitinous shells	186:205	the chitinous shells of crustacean zooplankton in its aquatic reservoir	186:256	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	12	74	with	interactions	1666:1677	arg1	chitin					1684:1689	chitin	1684:1689	chitin	1684:1689	V. cholerae interactions with chitin are critical for the survival, evolution, and waterborne transmission of this pathogen.					
32651201	0	75	theme	Quorum	17:22	arg1	Sensing					24:30	Species-Specific Quorum Sensing	0:30	Species-Specific Quorum Sensing	0:30	Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.					
32651201	9	76	theme	pathogen	1269:1276	arg1	fitness					1253:1259	the fitness	1249:1259	the fitness of this pathogen	1249:1276	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	3	77	theme	chitin-induced	396:409	arg1	changes					411:417	chitin-induced changes	396:417	chitin-induced changes in gene expression in this pathogen	396:453	This is due, in part, to chitin-induced changes in gene expression in this pathogen.					
32651201	4	78	theme	utilization	562:572	arg1	chb					582:584	chb	582:584	chb	582:584	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	4	78	theme	utilization	562:572	arg1	operon					574:579	the chitobiose utilization operon	547:579	the chitobiose utilization operon (chb)	547:585	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	4	78	theme	utilization	562:572	arg1	locus					540:544	one locus	536:544	one locus	536:544	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	5	79	theme	direct	771:776	arg1	repressor					778:786	a direct repressor	769:786	a direct repressor of the chb operon	769:804	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	5	79	theme	direct	771:776	arg1	regulator					731:739	the master regulator	720:739	the master regulator of quorum sensing	720:757	Through a series of genetic screens, we identified that the master regulator of quorum sensing, HapR, is a direct repressor of the chb operon.					
32651201	1	80	theme	facultative	106:116	arg1	pathogen					118:125	The marine facultative pathogen	95:125	The marine facultative pathogen Vibrio cholerae	95:141	The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir.					
32651201	0	81	theme	Chitobiose	46:55	arg1	Locus					69:73	the Chitobiose Utilization Locus	42:73	the Chitobiose Utilization Locus in Vibrio cholerae	42:92	Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.					
32651201	9	82	from	multispecies	1362:1373	arg1	live					1336:1339	live	1336:1339	live	1336:1339	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	4	83	theme	locus	540:544	arg1	expression					522:531	chitin-induced expression	507:531	chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide	507:661	Here, we sought to identify factors that influence chitin-induced expression of one locus, the chitobiose utilization operon (chb), which is required for the uptake and catabolism of the chitin disaccharide.					
32651201	11	84	theme	aquatic	1635:1641	arg1	reservoir					1643:1651	its aquatic reservoir	1631:1651	its aquatic reservoir	1631:1651	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	11	85	theme	Vibrio	1549:1554	arg1	cholerae					1556:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	9	86	from	multicellular	1344:1356	arg1	live					1336:1339	live	1336:1339	live	1336:1339	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	11	87	from	shells	1606:1611	arg1	reservoir					1643:1651	its aquatic reservoir	1631:1651	its aquatic reservoir	1631:1651	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	11	88	theme	marine	1533:1538	arg1	cholerae					1556:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae	1529:1563	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32651201	9	89	from	live	1336:1339	arg1	multicellular					1344:1356	multicellular	1344:1356	multicellular	1344:1356	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	9	89	from	live	1336:1339	arg1	multispecies					1362:1373	multispecies	1362:1373	multispecies	1362:1373	This phenomenon of species-specific regulation may enhance the fitness of this pathogen in its environmental niche.IMPORTANCE In nature, bacteria live in multicellular and multispecies communities.					
32651201	11	90	theme	chitinous	1596:1604	arg1	shells					1606:1611	the chitinous shells	1592:1611	the chitinous shells of crustaceans in its aquatic reservoir	1592:1651	The marine pathogen Vibrio cholerae is found in communities on the chitinous shells of crustaceans in its aquatic reservoir.					
32224171	5	0	theme	kinetic	735:741	arg1	model					743:747	the pseudo-first order kinetic model	712:747	the pseudo-first order kinetic model	712:747	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	4	1	theme	adsorption	568:577	arg1	capacity					579:586	the maximum adsorption capacity	556:586	the maximum adsorption capacity	556:586	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	5	2	theme	zein-graft-β-CD	673:687	arg1	film					689:692	zein-graft-β-CD film	673:692	zein-graft-β-CD film	673:692	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	4	3	theme	maximum	560:566	arg1	capacity					579:586	the maximum adsorption capacity	556:586	the maximum adsorption capacity	556:586	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	2	4	from	solubility	368:377	arg1	water					382:386	water	382:386	water	382:386	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	2	5	theme	glass	328:332	arg1	temperature					345:355	higher glass transition temperature	321:355	higher glass transition temperature	321:355	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	2	6	theme	higher	361:366	arg1	solubility					368:377	higher solubility	361:377	higher solubility in water	361:386	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	4	7	theme	adsorption	529:538	arg1	properties					540:549	the best adsorption properties	520:549	the best adsorption properties	520:549	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	2	8	theme	higher	321:326	arg1	temperature					345:355	higher glass transition temperature	321:355	higher glass transition temperature	321:355	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	6	9	theme	adsorption	800:809	arg1	capacity					811:818	an adsorption capacity	797:818	an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film	797:855	In addition, the zein-graft-β-CD film retained an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film after three reuses.					
32224171	3	10	theme	Zein-graft-β-CD	389:403	arg1	films					405:409	Zein-graft-β-CD films	389:409	Zein-graft-β-CD films	389:409	Zein-graft-β-CD films, prepared by solvent evaporation casting, showed excellent capacity for cholesterol adsorption.					
32224171	4	11	theme	best	524:527	arg1	properties					540:549	the best adsorption properties	520:549	the best adsorption properties	520:549	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	8	12	theme	food	1134:1137	arg1	industry					1139:1146	the food industry	1130:1146	the food industry	1130:1146	These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.					
32224171	1	13	from	temperature	183:193	arg1	adsorption					164:173	cholesterol adsorption	152:173	cholesterol adsorption at room temperature	152:193	A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature.					
32224171	1	14	theme	novel	85:89	arg1	film					127:130	A novel bio-based zein-graft-β-cyclodextrin film	83:130	A novel bio-based zein-graft-β-cyclodextrin film	83:130	A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature.					
32224171	7	15	theme	F1:1	948:951	arg1	degradation					954:964	the film (F1:1) degradation	938:964	the film (F1:1) degradation	938:964	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	3	16	theme	excellent	460:468	arg1	capacity					470:477	excellent capacity	460:477	excellent capacity for cholesterol adsorption	460:504	Zein-graft-β-CD films, prepared by solvent evaporation casting, showed excellent capacity for cholesterol adsorption.					
32224171	5	17	theme	adsorption	637:646	arg1	mechanism					660:668	The adsorption cholesterol mechanism	633:668	The adsorption cholesterol mechanism of zein-graft-β-CD film	633:692	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	1	18	theme	bio-based	91:99	arg1	film					127:130	A novel bio-based zein-graft-β-cyclodextrin film	83:130	A novel bio-based zein-graft-β-cyclodextrin film	83:130	A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature.					
32224171	3	19	theme	evaporation	432:442	arg1	casting					444:450	solvent evaporation casting	424:450	solvent evaporation casting	424:450	Zein-graft-β-CD films, prepared by solvent evaporation casting, showed excellent capacity for cholesterol adsorption.					
32224171	7	20	dep	enzyme	893:898	arg1	papain					909:914	1 mg/mL papain	901:914	1 mg/mL papain	901:914	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	7	20	dep	enzyme	893:898	arg1	amylase					928:934	1 mg/mL amylase	920:934	1 mg/mL amylase	920:934	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	5	21	theme	pseudo-first	716:727	arg1	model					743:747	the pseudo-first order kinetic model	712:747	the pseudo-first order kinetic model	712:747	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	5	22	theme	cholesterol	648:658	arg1	mechanism					660:668	The adsorption cholesterol mechanism	633:668	The adsorption cholesterol mechanism of zein-graft-β-CD film	633:692	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	1	23	theme	zein-graft-β-cyclodextrin	101:125	arg1	film					127:130	A novel bio-based zein-graft-β-cyclodextrin film	83:130	A novel bio-based zein-graft-β-cyclodextrin film	83:130	A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature.					
32224171	7	24	theme	film	942:945	arg1	degradation					954:964	the film (F1:1) degradation	938:964	the film (F1:1) degradation	938:964	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	0	25	theme	Adsorptive	0:9	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of cholesterol by biodegradable zein-graft-β-cyclodextrin film	0:80	Adsorptive removal of cholesterol by biodegradable zein-graft-β-cyclodextrin film.					
32224171	8	26	contain	has	1040:1042	arg2	potential					1044:1052	potential	1044:1052	potential as a nature-based adsorbent for cholesterol adsorption	1044:1107	These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.					
32224171	8	26	contain	has	1040:1042	arg1	film					1035:1038	zein-graft-β-CD film	1019:1038	zein-graft-β-CD film	1019:1038	These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.					
32224171	5	27	theme	order	729:733	arg1	model					743:747	the pseudo-first order kinetic model	712:747	the pseudo-first order kinetic model	712:747	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	6	28	theme	zein-graft-β-CD	767:781	arg1	film					783:786	the zein-graft-β-CD film	763:786	the zein-graft-β-CD film	763:786	In addition, the zein-graft-β-CD film retained an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film after three reuses.					
32224171	3	29	theme	cholesterol	483:493	arg1	adsorption					495:504	cholesterol adsorption	483:504	cholesterol adsorption	483:504	Zein-graft-β-CD films, prepared by solvent evaporation casting, showed excellent capacity for cholesterol adsorption.					
32224171	0	30	theme	cholesterol	22:32	arg1	removal					11:17	Adsorptive removal	0:17	Adsorptive removal of cholesterol by biodegradable zein-graft-β-cyclodextrin film	0:80	Adsorptive removal of cholesterol by biodegradable zein-graft-β-cyclodextrin film.					
32224171	4	31	theme	cholesterol/g	613:625	arg1	film					627:630	5.70 ± 0.56 mg cholesterol/g film	598:630	5.70 ± 0.56 mg cholesterol/g film	598:630	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	4	32	contain	has	516:518	arg1	F1:1					511:514	The F1:1	507:514	The F1:1	507:514	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	4	32	contain	has	516:518	arg2	properties					540:549	the best adsorption properties	520:549	the best adsorption properties	520:549	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	3	33	theme	solvent	424:430	arg1	casting					444:450	solvent evaporation casting	424:450	solvent evaporation casting	424:450	Zein-graft-β-CD films, prepared by solvent evaporation casting, showed excellent capacity for cholesterol adsorption.					
32224171	2	34	theme	transition	334:343	arg1	temperature					345:355	higher glass transition temperature	321:355	higher glass transition temperature	321:355	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	7	35	theme	1 mg/mL	920:926	arg1	amylase					928:934	1 mg/mL amylase	920:934	1 mg/mL amylase	920:934	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	0	36	theme	biodegradable	37:49	arg1	film					77:80	biodegradable zein-graft-β-cyclodextrin film	37:80	biodegradable zein-graft-β-cyclodextrin film	37:80	Adsorptive removal of cholesterol by biodegradable zein-graft-β-cyclodextrin film.					
32224171	7	37	theme	1 mg/mL	901:907	arg1	papain					909:914	1 mg/mL papain	901:914	1 mg/mL papain	901:914	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	2	38	theme	Maillard	264:271	arg1	reaction					273:280	the Maillard reaction	260:280	the Maillard reaction	260:280	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	8	39	theme	zein-graft-β-CD	1019:1033	arg1	film					1035:1038	zein-graft-β-CD film	1019:1038	zein-graft-β-CD film	1019:1038	These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.					
32224171	7	40	theme	composite	883:891	arg1	enzyme					893:898	composite enzyme	883:898	composite enzyme (1 mg/mL papain and 1 mg/mL amylase)	883:935	Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week.					
32224171	2	41	theme	β-cyclodextrin	214:227	arg1	Immobilization					196:209	Immobilization	196:209	Immobilization of β-cyclodextrin (β-CD) to zein	196:242	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	0	42	theme	zein-graft-β-cyclodextrin	51:75	arg1	film					77:80	biodegradable zein-graft-β-cyclodextrin film	37:80	biodegradable zein-graft-β-cyclodextrin film	37:80	Adsorptive removal of cholesterol by biodegradable zein-graft-β-cyclodextrin film.					
32224171	4	43	theme	5.70 ± 0.56 mg	598:611	arg1	film					627:630	5.70 ± 0.56 mg cholesterol/g film	598:630	5.70 ± 0.56 mg cholesterol/g film	598:630	The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film.					
32224171	1	44	theme	cholesterol	152:162	arg1	adsorption					164:173	cholesterol adsorption	152:173	cholesterol adsorption at room temperature	152:193	A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature.					
32224171	8	45	theme	cholesterol	1086:1096	arg1	adsorption					1098:1107	cholesterol adsorption	1086:1107	cholesterol adsorption	1086:1107	These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.					
32224171	2	46	theme	zein-graft-β-CD	290:304	arg1	powders					306:312	the zein-graft-β-CD powders	286:312	the zein-graft-β-CD powders	286:312	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
32224171	5	47	theme	film	689:692	arg1	mechanism					660:668	The adsorption cholesterol mechanism	633:668	The adsorption cholesterol mechanism of zein-graft-β-CD film	633:692	The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model.					
32224171	6	48	theme	film	852:855	arg1	capacity					811:818	an adsorption capacity	797:818	an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film	797:855	In addition, the zein-graft-β-CD film retained an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film after three reuses.					
32224171	6	49	theme	cholesterol/g	838:850	arg1	film					852:855	3.10 ± 0.89 mg cholesterol/g film	823:855	3.10 ± 0.89 mg cholesterol/g film	823:855	In addition, the zein-graft-β-CD film retained an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film after three reuses.					
32224171	8	50	used	used	1122:1125	arg2	film					1035:1038	zein-graft-β-CD film	1019:1038	zein-graft-β-CD film	1019:1038	These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.					
32224171	6	51	theme	3.10 ± 0.89 mg	823:836	arg1	film					852:855	3.10 ± 0.89 mg cholesterol/g film	823:855	3.10 ± 0.89 mg cholesterol/g film	823:855	In addition, the zein-graft-β-CD film retained an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film after three reuses.					
32224171	1	52	theme	room	178:181	arg1	temperature					183:193	room temperature	178:193	room temperature	178:193	A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature.					
32224171	2	53	from	temperature	345:355	arg1	water					382:386	water	382:386	water	382:386	Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water.					
31981703	7	0	theme	desired	1454:1460	arg1	characteristics					1462:1476	the desired characteristics	1450:1476	the desired characteristics	1450:1476	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	6	1	theme	screw	1242:1246	arg1	speed					1248:1252	screw speed	1242:1252	screw speed	1242:1252	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	8	2	theme	limited	1662:1668	arg1	capacity					1677:1684	the limited drying capacity	1658:1684	the limited drying capacity of the dryer unit of the Consigma system	1658:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	4	3	theme	similar	858:864	arg1	ratios					911:916	similar to significantly lower liquid to solid (L/S) ratios	858:916	similar to significantly lower liquid to solid (L/S) ratios	858:916	Compared to the previous formulation, using HPMC as binder, all four formulations with PVP yielded significantly stronger granules at similar to significantly lower liquid to solid (L/S) ratios.					
31981703	1	4	theme	formulation	273:283	arg1	aspect					285:290	the formulation aspect	269:290	the formulation aspect of these techniques, such as twin screw wet granulation,	269:347	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	7	5	theme	binder	1378:1383	arg1	addition					1385:1392	wet binder addition	1374:1392	wet binder addition	1374:1392	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	7	6	theme	required	1420:1427	arg1	ratio					1433:1437	the required L/S ratio	1416:1437	the required L/S ratio to achieve the desired characteristics	1416:1476	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	5	7	dep	designs	992:998	arg1	replicate					951:959	replicate	951:959	replicate	951:959	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	6	8	theme	granule	1196:1202	arg1	characteristics					1204:1218	granule characteristics	1196:1218	granule characteristics	1196:1218	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	8	9	theme	unit	1699:1702	arg1	capacity					1677:1684	the limited drying capacity	1658:1684	the limited drying capacity of the dryer unit of the Consigma system	1658:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	1	10	theme	several	176:182	arg1	products					189:196	several drug products	176:196	several drug products manufactured through continuous manufacturing techniques	176:253	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	4	11	theme	solid	899:903	arg1	ratios					911:916	similar to significantly lower liquid to solid (L/S) ratios	858:916	similar to significantly lower liquid to solid (L/S) ratios	858:916	Compared to the previous formulation, using HPMC as binder, all four formulations with PVP yielded significantly stronger granules at similar to significantly lower liquid to solid (L/S) ratios.					
31981703	0	12	theme	high-dosed	104:113	arg1	API					131:133	a high-dosed, poorly soluble API	102:133	a high-dosed, poorly soluble API	102:133	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	5	13	theme	speed	1037:1041	arg1	impact					1021:1026	the impact	1017:1026	the impact of screw speed and L/S ratio on granule quality attributes	1017:1085	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	1	14	theme	products	189:196	arg1	commercialization					155:171	the recent commercialization	144:171	the recent commercialization of several drug products manufactured through continuous manufacturing techniques	144:253	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	5	15	theme	L/S	1047:1049	arg1	ratio					1051:1055	L/S ratio	1047:1055	L/S ratio	1047:1055	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	3	16	theme	addition	625:632	arg1	method					634:639	the binder addition method	614:639	the binder addition method (wet or dry) as well as surfactant (SLS) addition	614:689	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	8	17	theme	system	1720:1725	arg1	unit					1699:1702	the dryer unit	1689:1702	the dryer unit of the Consigma system	1689:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	6	18	theme	significant	1174:1184	arg1	impact					1186:1191	the most significant impact	1165:1191	the most significant impact on granule characteristics	1165:1218	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	0	19	theme	API	131:133	arg1	granulation					87:97	granulation	87:97	granulation of a high-dosed, poorly soluble API	87:133	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	4	20	with	formulations	793:804	arg1	PVP					811:813	PVP	811:813	PVP	811:813	Compared to the previous formulation, using HPMC as binder, all four formulations with PVP yielded significantly stronger granules at similar to significantly lower liquid to solid (L/S) ratios.					
31981703	2	21	theme	high-dosed	513:522	arg1	mebendazole					544:554	the high-dosed, poorly soluble API mebendazole	509:554	the high-dosed, poorly soluble API mebendazole	509:554	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	6	22	from	impact	1186:1191	arg1	characteristics					1204:1218	granule characteristics	1196:1218	granule characteristics	1196:1218	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	0	23	theme	addition	52:59	arg1	method					61:66	binder addition method	45:66	binder addition method	45:66	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	1	24	theme	screw	326:330	arg1	granulation					336:346	twin screw wet granulation	321:346	twin screw wet granulation	321:346	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	5	25	from	impact	1021:1026	arg1	attributes					1076:1085	granule quality attributes	1060:1085	granule quality attributes	1060:1085	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	0	26	dep	high-dosed	104:113	arg1	soluble					123:129	soluble	123:129	soluble	123:129	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	2	27	theme	API	540:542	arg1	mebendazole					544:554	the high-dosed, poorly soluble API mebendazole	509:554	the high-dosed, poorly soluble API mebendazole	509:554	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	4	28	theme	previous	740:747	arg1	formulation					749:759	the previous formulation	736:759	the previous formulation	736:759	Compared to the previous formulation, using HPMC as binder, all four formulations with PVP yielded significantly stronger granules at similar to significantly lower liquid to solid (L/S) ratios.					
31981703	5	29	theme	quality	1068:1074	arg1	attributes					1076:1085	granule quality attributes	1060:1085	granule quality attributes	1060:1085	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	2	30	theme	several	457:463	arg1	formulations					471:482	several model formulations	457:482	several model formulations	457:482	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	2	31	theme	Previous	374:381	arg1	research					383:390	Previous research	374:390	Previous research	374:390	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	0	32	theme	Continuous	0:9	arg1	granulation					22:32	Continuous twin screw granulation	0:32	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.	0:134	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	7	33	theme	quality	1286:1292	arg1	attributes					1294:1303	Similar granule quality attributes	1270:1303	Similar granule quality attributes	1270:1303	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	2	34	theme	robust	425:430	arg1	formulation					441:451	a robust platform formulation	423:451	a robust platform formulation for several model formulations	423:482	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	2	34	theme	robust	425:430	arg1	lactose/MCC/HPMC					403:418	lactose/MCC/HPMC	403:418	lactose/MCC/HPMC	403:418	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	0	35	theme	screw	16:20	arg1	granulation					22:32	Continuous twin screw granulation	0:32	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.	0:134	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	7	36	theme	Similar	1270:1276	arg1	attributes					1294:1303	Similar granule quality attributes	1270:1303	Similar granule quality attributes	1270:1303	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	8	37	theme	other	1540:1544	arg1	formulations					1546:1557	processing other formulations	1529:1557	processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system	1529:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	5	38	theme	variables	1118:1126	arg1	effect					1092:1097	the effect	1088:1097	the effect of the formulation variables	1088:1126	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	3	39	theme	SLS	677:679	arg1	addition					682:689	surfactant (SLS) addition	665:689	the binder addition method (wet or dry) as well as surfactant (SLS) addition	614:689	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	1	40	theme	continuous	219:228	arg1	techniques					244:253	continuous manufacturing techniques	219:253	continuous manufacturing techniques	219:253	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	5	41	theme	center	961:966	arg1	each					1001:1004	each	1001:1004	each	1001:1004	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	5	41	theme	center	961:966	arg1	designs					992:998	center composite circumscribed designs	961:998	four replicate center composite circumscribed designs	946:998	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	8	42	theme	moisture	1585:1592	arg1	moisture					1585:1592	moisture	1585:1592	moisture	1585:1592	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	8	42	theme	moisture	1585:1592	arg1	amounts					1574:1580	high amounts	1569:1580	high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system	1569:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	3	43	theme	surfactant	665:674	arg1	addition					682:689	surfactant (SLS) addition	665:689	the binder addition method (wet or dry) as well as surfactant (SLS) addition	614:689	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	8	44	theme	high	1635:1638	arg1	throughput					1640:1649	a high throughput	1633:1649	a high throughput due to the limited drying capacity of the dryer unit of the Consigma system	1633:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	5	45	theme	circumscribed	978:990	arg1	each					1001:1004	each	1001:1004	each	1001:1004	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	5	45	theme	circumscribed	978:990	arg1	designs					992:998	center composite circumscribed designs	961:998	four replicate center composite circumscribed designs	946:998	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	6	46	theme	speed	1248:1252	arg1	negligible					1258:1267	negligible	1258:1267	negligible	1258:1267	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	6	46	theme	speed	1248:1252	arg1	effect					1232:1237	the effect	1228:1237	the effect of screw speed	1228:1252	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	8	47	theme	due	1651:1653	arg1	throughput					1640:1649	a high throughput	1633:1649	a high throughput due to the limited drying capacity of the dryer unit of the Consigma system	1633:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	4	48	theme	stronger	837:844	arg1	granules					846:853	significantly stronger granules	823:853	significantly stronger granules	823:853	Compared to the previous formulation, using HPMC as binder, all four formulations with PVP yielded significantly stronger granules at similar to significantly lower liquid to solid (L/S) ratios.					
31981703	7	49	theme	L/S	1429:1431	arg1	ratio					1433:1437	the required L/S ratio	1416:1437	the required L/S ratio to achieve the desired characteristics	1416:1476	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	8	50	theme	drying	1670:1675	arg1	capacity					1677:1684	the limited drying capacity	1658:1684	the limited drying capacity of the dryer unit of the Consigma system	1658:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	1	51	theme	recent	148:153	arg1	commercialization					155:171	the recent commercialization	144:171	the recent commercialization of several drug products manufactured through continuous manufacturing techniques	144:253	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	7	52	theme	addition	1385:1392	arg1	addition					1354:1361	the addition	1350:1361	the addition of SLS and wet binder addition	1350:1392	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	3	53	theme	current	587:593	arg1	research					595:602	current research	587:602	current research	587:602	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	7	54	theme	wet	1374:1376	arg1	addition					1385:1392	wet binder addition	1374:1392	wet binder addition	1374:1392	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	8	55	theme	dryer	1693:1697	arg1	unit					1699:1702	the dryer unit	1689:1702	the dryer unit of the Consigma system	1689:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	3	56	dep	method	634:639	arg1	dry					649:651	dry	649:651	dry	649:651	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	3	56	dep	method	634:639	arg1	wet					642:644	wet	642:644	wet	642:644	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	0	57	from	surfactants	72:82	arg1	granulation					87:97	granulation	87:97	granulation of a high-dosed, poorly soluble API	87:133	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	7	58	theme	SLS	1366:1368	arg1	addition					1354:1361	the addition	1350:1361	the addition of SLS and wet binder addition	1350:1392	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	1	59	from	knowledge	256:264	arg1	aspect					285:290	the formulation aspect	269:290	the formulation aspect of these techniques, such as twin screw wet granulation,	269:347	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	3	60	theme	binder	618:623	arg1	method					634:639	the binder addition method	614:639	the binder addition method (wet or dry) as well as surfactant (SLS) addition	614:689	Therefore, current research evaluated the binder addition method (wet or dry) as well as surfactant (SLS) addition when using PVP, instead of HPMC.					
31981703	2	61	theme	mebendazole	544:554	arg1	granulation					494:504	granulation	494:504	granulation of the high-dosed, poorly soluble API mebendazole	494:554	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	5	62	theme	ratio	1051:1055	arg1	impact					1021:1026	the impact	1017:1026	the impact of screw speed and L/S ratio on granule quality attributes	1017:1085	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	8	63	theme	Consigma	1711:1718	arg1	system					1720:1725	the Consigma system	1707:1725	the Consigma system	1707:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	0	64	theme	method	61:66	arg1	Impact					35:40	Impact	35:40	Impact of binder addition method	35:66	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	0	64	theme	method	61:66	arg1	surfactants					72:82	surfactants	72:82	surfactants on granulation of a high-dosed, poorly soluble API	72:133	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	5	65	theme	screw	1031:1035	arg1	speed					1037:1041	screw speed	1031:1041	screw speed	1031:1041	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	1	66	theme	drug	184:187	arg1	products					189:196	several drug products	176:196	several drug products manufactured through continuous manufacturing techniques	176:253	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	6	67	theme	L/S	1151:1153	arg1	ratio					1155:1159	L/S ratio	1151:1159	L/S ratio	1151:1159	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	1	68	theme	wet	332:334	arg1	granulation					336:346	twin screw wet granulation	321:346	twin screw wet granulation	321:346	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	8	69	theme	significant	1484:1494	arg1	reduction					1496:1504	This significant reduction	1479:1504	This significant reduction	1479:1504	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	4	70	dep	solid	899:903	arg1	to					896:897	to	896:897	to	896:897	Compared to the previous formulation, using HPMC as binder, all four formulations with PVP yielded significantly stronger granules at similar to significantly lower liquid to solid (L/S) ratios.					
31981703	5	71	theme	granule	1060:1066	arg1	attributes					1076:1085	granule quality attributes	1060:1085	granule quality attributes	1060:1085	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	0	72	from	Impact	35:40	arg1	granulation					87:97	granulation	87:97	granulation of a high-dosed, poorly soluble API	87:133	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	0	73	theme	twin	11:14	arg1	granulation					22:32	Continuous twin screw granulation	0:32	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.	0:134	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	7	74	theme	granule	1278:1284	arg1	attributes					1294:1303	Similar granule quality attributes	1270:1303	Similar granule quality attributes	1270:1303	Similar granule quality attributes were obtained for each formulation, although the addition of SLS and wet binder addition significantly reduced the required L/S ratio to achieve the desired characteristics.					
31981703	8	75	theme	processing	1529:1538	arg1	formulations					1546:1557	processing other formulations	1529:1557	processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system	1529:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	2	76	theme	platform	432:439	arg1	formulation					441:451	a robust platform formulation	423:451	a robust platform formulation for several model formulations	423:482	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	2	76	theme	platform	432:439	arg1	lactose/MCC/HPMC					403:418	lactose/MCC/HPMC	403:418	lactose/MCC/HPMC	403:418	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	1	77	theme	techniques	301:310	arg1	aspect					285:290	the formulation aspect	269:290	the formulation aspect of these techniques, such as twin screw wet granulation,	269:347	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	5	78	theme	designs	992:998	arg1	combination					931:941	the combination	927:941	the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes	927:1085	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	8	79	theme	high	1569:1572	arg1	moisture					1585:1592	moisture	1585:1592	moisture	1585:1592	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	8	79	theme	high	1569:1572	arg1	amounts					1574:1580	high amounts	1569:1580	high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system	1569:1725	This significant reduction could prove useful for processing other formulations requiring high amounts of moisture, which could otherwise not be dried at a high throughput due to the limited drying capacity of the dryer unit of the Consigma system.					
31981703	0	80	theme	binder	45:50	arg1	method					61:66	binder addition method	45:66	binder addition method	45:66	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	5	81	theme	formulation	1106:1116	arg1	variables					1118:1126	the formulation variables	1102:1126	the formulation variables	1102:1126	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	2	82	theme	model	465:469	arg1	formulations					471:482	several model formulations	457:482	several model formulations	457:482	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	6	83	contain	had	1161:1163	arg2	impact					1186:1191	the most significant impact	1165:1191	the most significant impact on granule characteristics	1165:1218	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	6	83	contain	had	1161:1163	arg1	ratio					1155:1159	L/S ratio	1151:1159	L/S ratio	1151:1159	Overall, L/S ratio had the most significant impact on granule characteristics whereas the effect of screw speed was negligible.					
31981703	1	84	theme	twin	321:324	arg1	granulation					336:346	twin screw wet granulation	321:346	twin screw wet granulation	321:346	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	2	85	dep	high-dosed	513:522	arg1	soluble					532:538	soluble	532:538	soluble	532:538	Previous research identified lactose/MCC/HPMC as a robust platform formulation for several model formulations, although granulation of the high-dosed, poorly soluble API mebendazole proved challenging.					
31981703	0	86	dep	granulation	22:32	arg1	Impact					35:40	Impact	35:40	Impact of binder addition method	35:66	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	0	86	dep	granulation	22:32	arg1	surfactants					72:82	surfactants	72:82	surfactants on granulation of a high-dosed, poorly soluble API	72:133	Continuous twin screw granulation: Impact of binder addition method and surfactants on granulation of a high-dosed, poorly soluble API.					
31981703	1	87	theme	manufacturing	230:242	arg1	techniques					244:253	continuous manufacturing techniques	219:253	continuous manufacturing techniques	219:253	Despite the recent commercialization of several drug products manufactured through continuous manufacturing techniques, knowledge on the formulation aspect of these techniques, such as twin screw wet granulation, is still rather limited.					
31981703	5	88	theme	composite	968:976	arg1	each					1001:1004	each	1001:1004	each	1001:1004	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
31981703	5	88	theme	composite	968:976	arg1	designs					992:998	center composite circumscribed designs	961:998	four replicate center composite circumscribed designs	946:998	Through the combination of four replicate center composite circumscribed designs, each evaluating the impact of screw speed and L/S ratio on granule quality attributes, the effect of the formulation variables was assessed.					
33045301	7	0	theme	cell	1211:1214	arg1	effects					1222:1228	The apoptotic and necrotic cell death effects	1184:1228	The apoptotic and necrotic cell death effects of AgNPs	1184:1237	The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry.					
33045301	3	1	theme	Ultraviolet	494:504	arg1	spectroscopy					514:525	Ultraviolet visible spectroscopy	494:525	Ultraviolet visible spectroscopy (UV-Vis spec) study	494:545	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	5	2	from	49 nm	937:941	arg1	ranges					919:924	size ranges	914:924	size ranges from 17 to 49 nm	914:941	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	1	3	theme	AgNPs	239:243	arg1	route					223:227	a well-organized, simplistic, and biological route	178:227	a well-organized, simplistic, and biological route of AgNPs (AgNPs)	178:244	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	9	4	theme	drug	1641:1644	arg1	development					1646:1656	the therapeutic drug development	1625:1656	the therapeutic drug development	1625:1656	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	4	5	theme	AgNPs	768:772	arg1	presence					774:781	AgNPs presence	768:781	AgNPs presence in the composites	768:799	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	8	6	theme	related	1304:1310	arg1	Bcl-xl					1427:1432	Bcl-xl	1427:1432	Bcl-xl	1427:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	caspase-3					1340:1348	caspase-3	1340:1348	caspase-3	1340:1348	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	caspase-9					1351:1359	caspase-9	1351:1359	caspase-9	1351:1359	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	proteins					1312:1319	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins	1278:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl	1278:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	Bax					1321:1323	Bax	1321:1323	Bax	1321:1323	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	Bcl-2					1417:1421	Bcl-2	1417:1421	Bcl-2	1417:1421	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	cytochrome-c					1326:1337	cytochrome-c	1326:1337	cytochrome-c	1326:1337	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	6	theme	related	1304:1310	arg1	PARP					1362:1365	PARP	1362:1365	PARP	1362:1365	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	1	7	theme	well-organized	180:193	arg1	route					223:227	a well-organized, simplistic, and biological route	178:227	a well-organized, simplistic, and biological route of AgNPs (AgNPs)	178:244	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	0	8	theme	human	119:123	arg1	cells					156:160	human hepatocellular carcinoma HepG2 cells	119:160	human hepatocellular carcinoma HepG2 cells	119:160	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	5	9	theme	colloidal	1031:1039	arg1	mixture					1041:1047	the colloidal mixture	1027:1047	the colloidal mixture	1027:1047	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	5	10	theme	TEM	810:812	arg1	studies					825:831	The SEM TEM analytical studies	802:831	The SEM TEM analytical studies	802:831	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	0	11	theme	carcinoma	140:148	arg1	cells					156:160	human hepatocellular carcinoma HepG2 cells	119:160	human hepatocellular carcinoma HepG2 cells	119:160	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	3	12	theme	present	587:593	arg1	AgNPs					581:585	AgNPs	581:585	AgNPs present in the colloidal solution	581:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	8	13	dep	proteins	1312:1319	arg1	Bcl-xl					1427:1432	Bcl-xl	1427:1432	Bcl-xl	1427:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	caspase-3					1340:1348	caspase-3	1340:1348	caspase-3	1340:1348	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	caspase-9					1351:1359	caspase-9	1351:1359	caspase-9	1351:1359	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	proteins					1312:1319	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins	1278:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl	1278:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	proteins					1408:1415	downregulated anti-apoptotic related proteins	1371:1415	downregulated anti-apoptotic related proteins	1371:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	Bax					1321:1323	Bax	1321:1323	Bax	1321:1323	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	Bcl-2					1417:1421	Bcl-2	1417:1421	Bcl-2	1417:1421	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	cytochrome-c					1326:1337	cytochrome-c	1326:1337	cytochrome-c	1326:1337	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	13	dep	proteins	1312:1319	arg1	PARP					1362:1365	PARP	1362:1365	PARP	1362:1365	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	5	14	theme	elements	1005:1012	arg1	percentage					973:982	the percentage	969:982	the percentage of weight and atomic elements available in the colloidal mixture	969:1047	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	0	15	from	activity	107:114	arg1	cells					156:160	human hepatocellular carcinoma HepG2 cells	119:160	human hepatocellular carcinoma HepG2 cells	119:160	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	5	16	dep	49 nm	937:941	arg1	to					934:935	to	934:935	to	934:935	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	5	17	theme	size	914:917	arg1	ranges					919:924	size ranges	914:924	size ranges from 17 to 49 nm	914:941	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	4	18	theme	chitin	710:715	arg1	AgNPs					730:734	the chitin and biogenic AgNPs	706:734	AgNPs	730:734	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	8	19	theme	upregulated	1282:1292	arg1	Bcl-xl					1427:1432	Bcl-xl	1427:1432	Bcl-xl	1427:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	caspase-3					1340:1348	caspase-3	1340:1348	caspase-3	1340:1348	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	caspase-9					1351:1359	caspase-9	1351:1359	caspase-9	1351:1359	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	proteins					1312:1319	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins	1278:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl	1278:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	Bax					1321:1323	Bax	1321:1323	Bax	1321:1323	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	Bcl-2					1417:1421	Bcl-2	1417:1421	Bcl-2	1417:1421	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	cytochrome-c					1326:1337	cytochrome-c	1326:1337	cytochrome-c	1326:1337	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	19	theme	upregulated	1282:1292	arg1	PARP					1362:1365	PARP	1362:1365	PARP	1362:1365	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	7	20	theme	necrotic	1202:1209	arg1	effects					1222:1228	The apoptotic and necrotic cell death effects	1184:1228	The apoptotic and necrotic cell death effects of AgNPs	1184:1237	The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry.					
33045301	3	21	attach	present	587:593	arg1	solution					612:619	the colloidal solution	598:619	the colloidal solution	598:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	3	21	attach	present	587:593	arg2	AgNPs					581:585	AgNPs	581:585	AgNPs present in the colloidal solution	581:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	2	22	theme	HepG2	480:484	arg1	cells					487:491	human hepatocarcinoma (HepG2) cells	457:491	human hepatocarcinoma (HepG2) cells	457:491	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	8	23	theme	anticancer	1465:1474	arg1	potential					1476:1484	the anticancer potential	1461:1484	the anticancer potential of AgNPs	1461:1493	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	4	24	theme	chemical	677:684	arg1	interaction					686:696	a strong chemical interaction	668:696	a strong chemical interaction between the chitin and biogenic AgNPs	668:734	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	5	25	from	available	1014:1022	arg1	mixture					1041:1047	the colloidal mixture	1027:1047	the colloidal mixture	1027:1047	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	5	26	theme	shape	881:885	arg1	structure					899:907	a spherical shape crystalline structure	869:907	a spherical shape crystalline structure	869:907	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	5	27	from	mixture	1041:1047	arg1	available					1014:1022	available	1014:1022	available	1014:1022	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	4	28	theme	biogenic	721:728	arg1	AgNPs					730:734	the chitin and biogenic AgNPs	706:734	AgNPs	730:734	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	3	29	from	solution	612:619	arg1	present					587:593	present	587:593	present	587:593	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	0	30	theme	Shrimp	0:5	arg1	shells					7:12	Shrimp shells	0:12	Shrimp shells	0:12	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	9	31	theme	anticancer	1554:1563	arg1	activity					1565:1572	significant anticancer activity	1542:1572	significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future	1542:1682	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	2	32	theme	biogenic	420:427	arg1	AgNPs					429:433	synthesized biogenic AgNPs	408:433	synthesized biogenic AgNPs	408:433	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	1	33	theme	shrimp	268:273	arg1	shell					275:279	shrimp shell	268:279	shrimp shell extracted chitin	268:296	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	3	34	dep	spectroscopy	514:525	arg1	spec					535:538	spec	535:538	spec	535:538	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	2	35	theme	hepatocarcinoma	463:477	arg1	cells					487:491	human hepatocarcinoma (HepG2) cells	457:491	human hepatocarcinoma (HepG2) cells	457:491	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	3	36	from	present	587:593	arg1	solution					612:619	the colloidal solution	598:619	the colloidal solution	598:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	2	37	theme	anticancer	384:393	arg1	potential					395:403	the anticancer potential	380:403	the anticancer potential of synthesized biogenic AgNPs	380:433	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	5	38	theme	weight	987:992	arg1	percentage					973:982	the percentage	969:982	the percentage of weight and atomic elements available in the colloidal mixture	969:1047	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	7	39	theme	flow	1262:1265	arg1	cytometry					1267:1275	flow cytometry	1262:1275	flow cytometry	1262:1275	The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry.					
33045301	3	40	theme	single	625:630	arg1	peak					632:635	single peak	625:635	single peak	625:635	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	3	40	theme	single	625:630	arg1	development					566:576	the development	562:576	the development of AgNPs present in the colloidal solution	562:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	8	41	theme	downregulated	1371:1383	arg1	proteins					1408:1415	downregulated anti-apoptotic related proteins	1371:1415	downregulated anti-apoptotic related proteins	1371:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	0	42	theme	nanoparticle	41:52	arg1	synthesis					54:62	silver nanoparticle synthesis	34:62	silver nanoparticle synthesis	34:62	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	5	43	theme	atomic	998:1003	arg1	elements					1005:1012	atomic elements	998:1012	atomic elements	998:1012	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	6	44	theme	cytotoxic	1104:1112	arg1	effect					1114:1119	significant cytotoxic effect	1092:1119	significant cytotoxic effect on the HepG2 cells	1092:1138	Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml.					
33045301	8	45	theme	related	1400:1406	arg1	proteins					1408:1415	downregulated anti-apoptotic related proteins	1371:1415	downregulated anti-apoptotic related proteins	1371:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	1	46	theme	biological	212:221	arg1	route					223:227	a well-organized, simplistic, and biological route	178:227	a well-organized, simplistic, and biological route of AgNPs (AgNPs)	178:244	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	9	47	theme	major	1611:1615	arg1	role					1617:1620	major role	1611:1620	major role	1611:1620	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	3	48	from	446 nm	640:645	arg1	peak					632:635	single peak	625:635	single peak	625:635	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	3	48	from	446 nm	640:645	arg1	development					566:576	the development	562:576	the development of AgNPs present in the colloidal solution	562:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	3	49	theme	visible	506:512	arg1	spectroscopy					514:525	Ultraviolet visible spectroscopy	494:525	Ultraviolet visible spectroscopy (UV-Vis spec) study	494:545	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	6	50	theme	synthesized	1067:1077	arg1	AgNPs					1079:1083	the synthesized AgNPs	1063:1083	the synthesized AgNPs	1063:1083	Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml.					
33045301	9	51	theme	therapeutic	1629:1639	arg1	development					1646:1656	the therapeutic drug development	1625:1656	the therapeutic drug development	1625:1656	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	7	52	theme	death	1216:1220	arg1	effects					1222:1228	The apoptotic and necrotic cell death effects	1184:1228	The apoptotic and necrotic cell death effects of AgNPs	1184:1237	The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry.					
33045301	5	53	dep	confirmed	833:841	arg1	had					865:867	had	865:867	confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm	833:941	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	6	54	theme	IC50	1148:1151	arg1	value					1153:1157	an IC50 value	1145:1157	an IC50 value shown at 57 ± 1.5 μg/ml	1145:1181	Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml.					
33045301	7	55	theme	apoptotic	1188:1196	arg1	effects					1222:1228	The apoptotic and necrotic cell death effects	1184:1228	The apoptotic and necrotic cell death effects of AgNPs	1184:1237	The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry.					
33045301	5	56	theme	synthesized	847:857	arg1	AgNPs					859:863	the synthesized AgNPs	843:863	the synthesized AgNPs	843:863	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	6	57	theme	HepG2	1128:1132	arg1	cells					1134:1138	the HepG2 cells	1124:1138	the HepG2 cells	1124:1138	Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml.					
33045301	4	58	theme	FTIR	648:651	arg1	results					653:659	FTIR results	648:659	FTIR results	648:659	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	0	59	theme	hepatocellular	125:138	arg1	cells					156:160	human hepatocellular carcinoma HepG2 cells	119:160	human hepatocellular carcinoma HepG2 cells	119:160	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	5	60	theme	SEM	806:808	arg1	studies					825:831	The SEM TEM analytical studies	802:831	The SEM TEM analytical studies	802:831	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	Bcl-xl					1427:1432	Bcl-xl	1427:1432	Bcl-xl	1427:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	caspase-3					1340:1348	caspase-3	1340:1348	caspase-3	1340:1348	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	caspase-9					1351:1359	caspase-9	1351:1359	caspase-9	1351:1359	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	proteins					1312:1319	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins	1278:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl	1278:1432	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	Bax					1321:1323	Bax	1321:1323	Bax	1321:1323	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	Bcl-2					1417:1421	Bcl-2	1417:1421	Bcl-2	1417:1421	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	cytochrome-c					1326:1337	cytochrome-c	1326:1337	cytochrome-c	1326:1337	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	8	61	theme	apoptotic	1294:1302	arg1	PARP					1362:1365	PARP	1362:1365	PARP	1362:1365	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	0	62	theme	HepG2	150:154	arg1	cells					156:160	human hepatocellular carcinoma HepG2 cells	119:160	human hepatocellular carcinoma HepG2 cells	119:160	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	3	63	theme	AgNPs	581:585	arg1	peak					632:635	single peak	625:635	single peak	625:635	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	3	63	theme	AgNPs	581:585	arg1	development					566:576	the development	562:576	the development of AgNPs present in the colloidal solution	562:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	5	64	theme	analytical	814:823	arg1	studies					825:831	The SEM TEM analytical studies	802:831	The SEM TEM analytical studies	802:831	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	8	65	theme	cancer	1437:1442	arg1	cells					1444:1448	cancer cells	1437:1448	cancer cells	1437:1448	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	5	66	theme	crystalline	887:897	arg1	structure					899:907	a spherical shape crystalline structure	869:907	a spherical shape crystalline structure	869:907	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	5	67	theme	available	1014:1022	arg1	weight					987:992	weight	987:992	weight	987:992	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	3	68	theme	colloidal	602:610	arg1	solution					612:619	the colloidal solution	598:619	the colloidal solution	598:619	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
33045301	6	69	theme	significant	1092:1102	arg1	effect					1114:1119	significant cytotoxic effect	1092:1119	significant cytotoxic effect on the HepG2 cells	1092:1138	Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml.					
33045301	4	70	from	presence	774:781	arg1	composites					790:799	the composites	786:799	the composites	786:799	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	0	71	from	chitin	24:29	arg1	synthesis					54:62	silver nanoparticle synthesis	34:62	silver nanoparticle synthesis	34:62	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	1	72	theme	capping	311:317	arg1	factor					335:340	reducing, capping and stabilizing factor	301:340	reducing, capping and stabilizing factor under the optimized conditions	301:371	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	5	73	theme	spherical	871:879	arg1	structure					899:907	a spherical shape crystalline structure	869:907	a spherical shape crystalline structure	869:907	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	8	74	theme	AgNPs	1489:1493	arg1	potential					1476:1484	the anticancer potential	1461:1484	the anticancer potential of AgNPs	1461:1493	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	4	75	theme	strong	670:675	arg1	interaction					686:696	a strong chemical interaction	668:696	a strong chemical interaction between the chitin and biogenic AgNPs	668:734	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	0	76	theme	anticancer	96:105	arg1	activity					107:114	anticancer activity	96:114	anticancer activity in human hepatocellular carcinoma HepG2 cells	96:160	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	1	77	theme	stabilizing	323:333	arg1	factor					335:340	reducing, capping and stabilizing factor	301:340	reducing, capping and stabilizing factor under the optimized conditions	301:371	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	1	78	theme	simplistic	196:205	arg1	route					223:227	a well-organized, simplistic, and biological route	178:227	a well-organized, simplistic, and biological route of AgNPs (AgNPs)	178:244	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	5	79	theme	EDX	944:946	arg1	study					948:952	EDX study	944:952	EDX study	944:952	The SEM TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture.					
33045301	4	80	theme	XRD	746:748	arg1	studies					750:756	XRD studies	746:756	XRD studies	746:756	FTIR results showed a strong chemical interaction between the chitin and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites.					
33045301	1	81	theme	optimized	352:360	arg1	conditions					362:371	the optimized conditions	348:371	the optimized conditions	348:371	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	9	82	contain	possess	1534:1540	arg1	AgNPs					1528:1532	the AgNPs	1524:1532	the AgNPs	1524:1532	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	9	82	contain	possess	1534:1540	arg2	activity					1565:1572	significant anticancer activity	1542:1572	significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future	1542:1682	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	9	83	theme	significant	1542:1552	arg1	activity					1565:1572	significant anticancer activity	1542:1572	significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future	1542:1682	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	2	84	theme	AgNPs	429:433	arg1	potential					395:403	the anticancer potential	380:403	the anticancer potential of synthesized biogenic AgNPs	380:433	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	2	85	theme	synthesized	408:418	arg1	AgNPs					429:433	synthesized biogenic AgNPs	408:433	synthesized biogenic AgNPs	408:433	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	0	86	theme	silver	34:39	arg1	synthesis					54:62	silver nanoparticle synthesis	34:62	silver nanoparticle synthesis	34:62	Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells.					
33045301	9	87	theme	HepG2	1582:1586	arg1	cells					1588:1592	HepG2 cells	1582:1592	HepG2 cells	1582:1592	These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.					
33045301	2	88	theme	human	457:461	arg1	cells					487:491	human hepatocarcinoma (HepG2) cells	457:491	human hepatocarcinoma (HepG2) cells	457:491	Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.					
33045301	6	89	from	effect	1114:1119	arg1	cells					1134:1138	the HepG2 cells	1124:1138	the HepG2 cells	1124:1138	Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5 μg/ml.					
33045301	1	90	theme	reducing	301:308	arg1	factor					335:340	reducing, capping and stabilizing factor	301:340	reducing, capping and stabilizing factor under the optimized conditions	301:371	In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions.					
33045301	7	91	theme	AgNPs	1233:1237	arg1	effects					1222:1228	The apoptotic and necrotic cell death effects	1184:1228	The apoptotic and necrotic cell death effects of AgNPs	1184:1237	The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow cytometry.					
33045301	8	92	theme	anti-apoptotic	1385:1398	arg1	proteins					1408:1415	downregulated anti-apoptotic related proteins	1371:1415	downregulated anti-apoptotic related proteins	1371:1415	The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential of AgNPs.					
33045301	3	93	theme	spectroscopy	514:525	arg1	study					541:545	Ultraviolet visible spectroscopy (UV-Vis spec) study	494:545	Ultraviolet visible spectroscopy (UV-Vis spec) study	494:545	Ultraviolet visible spectroscopy (UV-Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm.					
34399623	6	0	theme	streptococci	950:961	arg1	abundance					925:933	the relative abundance	912:933	the relative abundance of mitis group streptococci	912:961	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	9	1	theme	negative	1380:1387	arg1	correlation					1389:1399	a significant negative correlation	1366:1399	a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice	1366:1467	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	12	2	from	reduction	1913:1921	arg1	lactobacilli					1957:1968	lactobacilli	1957:1968	lactobacilli	1957:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	2	from	reduction	1913:1921	arg1	enterococci					1926:1936	enterococci	1926:1936	enterococci	1926:1936	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	4	3	theme	mucosal	587:593	arg1	lesions					595:601	mucosal lesions	587:601	mucosal lesions	587:601	C. albicans burdens and mucosal lesions were evaluated in a mouse cortisone immunosuppression model amended with sucrose.					
34399623	13	4	from	role	2320:2323	arg1	virulence					2335:2343	fungal virulence	2328:2343	fungal virulence	2328:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	6	5	theme	bacterial	851:859	arg1	burdens					861:867	total bacterial burdens	845:867	total bacterial burdens	845:867	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	8	6	theme	C.	1200:1201	arg1	burdens					1212:1218	reduced C. albicans burdens	1192:1218	reduced C. albicans burdens	1192:1218	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	6	7	theme	mitis	938:942	arg1	streptococci					950:961	mitis group streptococci	938:961	mitis group streptococci	938:961	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	2	8	from	commensal	334:342	arg1	transition					303:312	the transition	299:312	the transition of C. albicans from commensal to pathogenic	299:356	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	11	9	theme	mouse	1623:1627	arg1	isolates					1658:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	16	10	theme	bacterial	2793:2801	arg1	microbiota					2803:2812	a mucosal bacterial microbiota	2783:2812	a mucosal bacterial microbiota conducive to virulence	2783:2835	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	13	11	with	environment	2141:2151	arg1	albicans					2192:2199	C. albicans	2189:2199	C. albicans	2189:2199	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	12	theme	oral	2071:2074	arg1	model					2086:2090	a mouse oral infection model	2063:2090	a mouse oral infection model	2063:2090	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	2	13	theme	bacteria	287:294	arg1	role					271:274	a significant role	257:274	a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic	257:356	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	8	14	theme	reduced	1224:1230	arg1	diversity					1248:1256	reduced bacterial alpha diversity	1224:1256	reduced bacterial alpha diversity	1224:1256	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	12	15	theme	changes	1850:1856	arg1	result					1840:1845	a result	1838:1845	a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1838:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	15	theme	changes	1850:1856	arg1	attenuation					1797:1807	the sucrose-induced attenuation	1777:1807	the sucrose-induced attenuation of C. albicans virulence	1777:1832	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	7	16	theme	enterococci	1131:1141	arg1	abundance					1118:1126	the relative abundance	1105:1126	the relative abundance of enterococci	1105:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	2	17	theme	albicans	320:327	arg1	transition					303:312	the transition	299:312	the transition of C. albicans from commensal to pathogenic	299:356	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	8	18	theme	alpha	1242:1246	arg1	diversity					1248:1256	reduced bacterial alpha diversity	1224:1256	reduced bacterial alpha diversity	1224:1256	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	11	19	theme	Lactobacillus	1634:1646	arg1	isolates					1658:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	4	20	theme	immunosuppression	639:655	arg1	model					657:661	a mouse cortisone immunosuppression model	621:661	a mouse cortisone immunosuppression model amended with sucrose	621:682	C. albicans burdens and mucosal lesions were evaluated in a mouse cortisone immunosuppression model amended with sucrose.					
34399623	7	21	theme	reduced	1049:1055	arg1	diversity					1073:1081	a significantly reduced bacterial alpha diversity	1033:1081	a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci	1033:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	8	22	theme	Lactobacillus	1316:1328	arg1	abundance					1303:1311	the relative abundance	1290:1311	the relative abundance of Lactobacillus	1290:1328	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	5	23	theme	16S	742:744	arg1	sequencing					756:765	16S rRNA gene sequencing and culture methods	742:785	sequencing	756:765	We also analyzed the mucosal bacterial composition using 16S rRNA gene sequencing and culture methods.					
34399623	7	24	theme	alpha	1067:1071	arg1	diversity					1073:1081	a significantly reduced bacterial alpha diversity	1033:1081	a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci	1033:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	8	25	from	increase	1278:1285	arg1	abundance					1303:1311	the relative abundance	1290:1311	the relative abundance of Lactobacillus	1290:1328	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	3	26	dep	C.	540:541	arg1	albicans					543:550	albicans	543:550	albicans	543:550	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	11	27	theme	johnsonii	1648:1656	arg1	isolates					1658:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	11	28	theme	coculture	1591:1599	arg1	experiments					1610:1620	coculture in vitro experiments	1591:1620	coculture in vitro experiments	1591:1620	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	5	29	theme	mucosal	706:712	arg1	composition					724:734	the mucosal bacterial composition	702:734	the mucosal bacterial composition	702:734	We also analyzed the mucosal bacterial composition using 16S rRNA gene sequencing and culture methods.					
34399623	13	30	theme	modulating	2212:2221	arg1	factors					2223:2229	local modulating factors	2206:2229	local modulating factors (availability of sucrose as a carbon source)	2206:2274	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	1	31	theme	oral	137:140	arg1	infection					142:150	the most prevalent oral infection	118:150	the most prevalent oral infection in immunocompromised patients	118:180	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	12	32	theme	bacterial	1873:1881	arg1	microbiome					1883:1892	the mucosal bacterial microbiome	1861:1892	the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1861:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	13	33	theme	mouse	2065:2069	arg1	model					2086:2090	a mouse oral infection model	2063:2090	a mouse oral infection model	2063:2090	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	2	34	theme	significant	259:269	arg1	role					271:274	a significant role	257:274	a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic	257:356	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	8	35	contain	had	1188:1190	arg1	mice					1183:1186	these mice	1177:1186	these mice	1177:1186	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	8	35	contain	had	1188:1190	arg2	burdens					1212:1218	reduced C. albicans burdens	1192:1218	reduced C. albicans burdens	1192:1218	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	8	35	contain	had	1188:1190	arg2	diversity					1248:1256	reduced bacterial alpha diversity	1224:1256	reduced bacterial alpha diversity	1224:1256	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	12	36	theme	C.	1812:1813	arg1	virulence					1824:1832	C. albicans virulence	1812:1832	C. albicans virulence	1812:1832	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	9	37	theme	correlation	1338:1348	arg1	networks					1350:1357	SparCC correlation networks	1331:1357	SparCC correlation networks	1331:1357	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	12	38	theme	virulence	1824:1832	arg1	result					1840:1845	a result	1838:1845	a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1838:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	38	theme	virulence	1824:1832	arg1	attenuation					1797:1807	the sucrose-induced attenuation	1777:1807	the sucrose-induced attenuation of C. albicans virulence	1777:1832	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	8	39	dep	C.	1200:1201	arg1	albicans					1203:1210	albicans	1203:1210	albicans	1203:1210	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	12	40	dep	C.	1812:1813	arg1	albicans					1815:1822	albicans	1815:1822	albicans	1815:1822	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	13	41	theme	bacterial	2038:2046	arg1	albicans					2003:2010	Candida albicans virulence and the mucosal bacterial composition	1995:2058	Candida albicans virulence and the mucosal bacterial composition	1995:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	41	theme	bacterial	2038:2046	arg1	composition					2048:2058	the mucosal bacterial composition	2026:2058	the mucosal bacterial composition	2026:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	5	42	theme	gene	751:754	arg1	sequencing					756:765	16S rRNA gene sequencing and culture methods	742:785	sequencing	756:765	We also analyzed the mucosal bacterial composition using 16S rRNA gene sequencing and culture methods.					
34399623	1	43	from	infection	142:150	arg1	patients					173:180	immunocompromised patients	155:180	immunocompromised patients	155:180	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	13	44	theme	Candida	1995:2001	arg1	albicans					2003:2010	Candida albicans virulence and the mucosal bacterial composition	1995:2058	Candida albicans virulence and the mucosal bacterial composition	1995:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	44	theme	Candida	1995:2001	arg1	composition					2048:2058	the mucosal bacterial composition	2026:2058	the mucosal bacterial composition	2026:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	44	theme	Candida	1995:2001	arg1	virulence					2012:2020	virulence	2012:2020	virulence	2012:2020	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	9	45	theme	significant	1368:1378	arg1	correlation					1389:1399	a significant negative correlation	1366:1399	a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice	1366:1467	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	14	46	from	changes	2361:2367	arg1	response					2408:2415	response	2408:2415	response to sucrose	2408:2426	We showed that changes in endogenous microbial communities in response to sucrose can lead to attenuation of fungal disease.					
34399623	14	46	from	changes	2361:2367	arg1	communities					2393:2403	endogenous microbial communities	2372:2403	endogenous microbial communities	2372:2403	We showed that changes in endogenous microbial communities in response to sucrose can lead to attenuation of fungal disease.					
34399623	3	47	dep	abundance	409:417	arg1	the					405:407	the	405:407	the	405:407	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	0	48	theme	Mucosal	0:6	arg1	Bacteria					8:15	Mucosal Bacteria	0:15	Mucosal Bacteria	0:15	Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis.					
34399623	2	49	from	role	271:274	arg1	transition					303:312	the transition	299:312	the transition of C. albicans from commensal to pathogenic	299:356	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	13	50	theme	fungal	2328:2333	arg1	virulence					2335:2343	fungal virulence	2328:2343	fungal virulence	2328:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	1	51	dep	associated	193:202	arg1	infection					142:150	the most prevalent oral infection	118:150	the most prevalent oral infection in immunocompromised patients	118:180	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	3	52	theme	bacterial	449:457	arg1	microbiota					459:468	the mucosal bacterial microbiota	437:468	the mucosal bacterial microbiota	437:468	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	0	53	theme	albicans	34:41	arg1	Virulence					43:51	Candida albicans Virulence	26:51	Candida albicans Virulence in Oropharyngeal Candidiasis	26:80	Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis.					
34399623	13	54	theme	bacterial	2291:2299	arg1	microbiome					2301:2310	the mucosal bacterial microbiome	2279:2310	the mucosal bacterial microbiome	2279:2310	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	55	dep	albicans	2003:2010	arg1	albicans					2003:2010	Candida albicans virulence and the mucosal bacterial composition	1995:2058	Candida albicans virulence and the mucosal bacterial composition	1995:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	55	dep	albicans	2003:2010	arg1	composition					2048:2058	the mucosal bacterial composition	2026:2058	the mucosal bacterial composition	2026:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	55	dep	albicans	2003:2010	arg1	virulence					2012:2020	virulence	2012:2020	virulence	2012:2020	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	12	56	from	changes	1850:1856	arg1	microbiome					1883:1892	the mucosal bacterial microbiome	1861:1892	the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1861:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	13	57	theme	host	2136:2139	arg1	immunosuppression					2154:2170	immunosuppression	2154:2170	immunosuppression	2154:2170	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	57	theme	host	2136:2139	arg1	environment					2141:2151	the host environment	2132:2151	the host environment (immunosuppression)	2132:2171	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	10	58	dep	C.	1541:1542	arg1	albicans					1544:1551	albicans	1544:1551	albicans	1544:1551	Depletion of lactobacilli with antibiotic treatment partially restored C. albicans burdens in mice receiving sucrose.					
34399623	15	59	theme	Lactobacillus	2491:2503	arg1	johnsonii					2505:2513	Lactobacillus johnsonii	2491:2513	Lactobacillus johnsonii	2491:2513	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	6	60	theme	alpha	881:885	arg1	diversity					887:895	reduced alpha diversity	873:895	reduced alpha diversity	873:895	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	12	61	from	increase	1945:1952	arg1	lactobacilli					1957:1968	lactobacilli	1957:1968	lactobacilli	1957:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	61	from	increase	1945:1952	arg1	enterococci					1926:1936	enterococci	1926:1936	enterococci	1926:1936	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	14	62	theme	disease	2462:2468	arg1	attenuation					2440:2450	attenuation	2440:2450	attenuation of fungal disease	2440:2468	We showed that changes in endogenous microbial communities in response to sucrose can lead to attenuation of fungal disease.					
34399623	11	63	theme	faecalis	1700:1707	arg1	isolates					1709:1716	Enterococcus faecalis isolates	1687:1716	Enterococcus faecalis isolates	1687:1716	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	3	64	theme	dietary	481:487	arg1	sucrose					489:495	dietary sucrose	481:495	dietary sucrose	481:495	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	3	65	theme	C.	540:541	arg1	virulence					552:560	C. albicans virulence	540:560	C. albicans virulence	540:560	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	13	66	theme	infection	2174:2182	arg1	effects					2121:2127	the effects	2117:2127	the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence	2117:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	16	67	theme	susceptible	2762:2772	arg1	host					2774:2777	a susceptible host	2760:2777	a susceptible host	2760:2777	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	3	68	theme	OPC	523:525	arg1	development					508:518	the development	504:518	the development of OPC	504:525	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	7	69	from	increase	1093:1100	arg1	abundance					1118:1126	the relative abundance	1105:1126	the relative abundance of enterococci	1105:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	11	70	theme	albicans	1725:1732	arg1	growth					1677:1682	growth	1677:1682	growth of Enterococcus faecalis isolates and C. albicans	1677:1732	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	10	71	with	Depletion	1470:1478	arg1	treatment					1512:1520	antibiotic treatment	1501:1520	antibiotic treatment	1501:1520	Depletion of lactobacilli with antibiotic treatment partially restored C. albicans burdens in mice receiving sucrose.					
34399623	6	72	theme	group	944:948	arg1	streptococci					950:961	mitis group streptococci	938:961	mitis group streptococci	938:961	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	16	73	theme	mucosal	2785:2791	arg1	microbiota					2803:2812	a mucosal bacterial microbiota	2783:2812	a mucosal bacterial microbiota conducive to virulence	2783:2835	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	13	74	dep	factors	2223:2229	arg1	availability					2232:2243	availability	2232:2243	availability of sucrose as a carbon source	2232:2273	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	75	theme	infection	2076:2084	arg1	model					2086:2090	a mouse oral infection model	2063:2090	a mouse oral infection model	2063:2090	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	6	76	theme	total	845:849	arg1	burdens					861:867	total bacterial burdens	845:867	total bacterial burdens	845:867	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	13	77	theme	carbon	2261:2266	arg1	source					2268:2273	a carbon source	2259:2273	a carbon source	2259:2273	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	8	78	theme	reduced	1192:1198	arg1	burdens					1212:1218	reduced C. albicans burdens	1192:1218	reduced C. albicans burdens	1192:1218	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	15	79	theme	bacteria	2631:2638	arg1	growth					2597:2602	the growth	2593:2602	the growth of potentially synergistic bacteria such as enterococci	2593:2658	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	11	80	theme	oral	1629:1632	arg1	isolates					1658:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	mouse oral Lactobacillus johnsonii isolates	1623:1665	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	13	81	from	effects	2121:2127	arg1	microbiome					2301:2310	the mucosal bacterial microbiome	2279:2310	the mucosal bacterial microbiome	2279:2310	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	81	from	effects	2121:2127	arg1	role					2320:2323	its role	2316:2323	its role on fungal virulence	2316:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	6	82	theme	relative	916:923	arg1	abundance					925:933	the relative abundance	912:933	the relative abundance of mitis group streptococci	912:961	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	10	83	theme	antibiotic	1501:1510	arg1	treatment					1512:1520	antibiotic treatment	1501:1520	antibiotic treatment	1501:1520	Depletion of lactobacilli with antibiotic treatment partially restored C. albicans burdens in mice receiving sucrose.					
34399623	15	84	dep	curtail	2519:2525	arg1	inhibiting					2582:2591	inhibiting	2582:2591	inhibiting the growth of potentially synergistic bacteria such as enterococci	2582:2658	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	15	84	dep	curtail	2519:2525	arg1	inhibiting					2553:2562	inhibiting	2553:2562	inhibiting its growth	2553:2573	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	12	85	theme	mucosal	1865:1871	arg1	microbiome					1883:1892	the mucosal bacterial microbiome	1861:1892	the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1861:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	5	86	theme	bacterial	714:722	arg1	composition					724:734	the mucosal bacterial composition	702:734	the mucosal bacterial composition	702:734	We also analyzed the mucosal bacterial composition using 16S rRNA gene sequencing and culture methods.					
34399623	6	87	theme	immunocompetent	791:805	arg1	mice					807:810	immunocompetent mice	791:810	immunocompetent mice	791:810	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	2	88	theme	C.	317:318	arg1	albicans					320:327	C. albicans	317:327	C. albicans	317:327	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	1	89	theme	Candida	209:215	arg1	albicans					217:224	Candida albicans	209:224	Candida albicans	209:224	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	8	90	theme	bacterial	1232:1240	arg1	diversity					1248:1256	reduced bacterial alpha diversity	1224:1256	reduced bacterial alpha diversity	1224:1256	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	13	91	theme	environment	2141:2151	arg1	effects					2121:2127	the effects	2117:2127	the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence	2117:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	8	92	theme	relative	1294:1301	arg1	abundance					1303:1311	the relative abundance	1290:1311	the relative abundance of Lactobacillus	1290:1328	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	13	93	theme	mucosal	2030:2036	arg1	albicans					2003:2010	Candida albicans virulence and the mucosal bacterial composition	1995:2058	Candida albicans virulence and the mucosal bacterial composition	1995:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	13	93	theme	mucosal	2030:2036	arg1	composition					2048:2058	the mucosal bacterial composition	2026:2058	the mucosal bacterial composition	2026:2058	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	12	94	from	result	1840:1845	arg1	microbiome					1883:1892	the mucosal bacterial microbiome	1861:1892	the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1861:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	14	95	theme	fungal	2455:2460	arg1	disease					2462:2468	fungal disease	2455:2468	fungal disease	2455:2468	We showed that changes in endogenous microbial communities in response to sucrose can lead to attenuation of fungal disease.					
34399623	13	96	theme	sucrose	2248:2254	arg1	availability					2232:2243	availability	2232:2243	availability of sucrose as a carbon source	2232:2273	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	7	97	theme	relative	1109:1116	arg1	abundance					1118:1126	the relative abundance	1105:1126	the relative abundance of enterococci	1105:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	7	98	dep	C.	991:992	arg1	albicans					994:1001	albicans	994:1001	albicans	994:1001	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	10	99	theme	lactobacilli	1483:1494	arg1	Depletion					1470:1478	Depletion	1470:1478	Depletion of lactobacilli with antibiotic treatment	1470:1520	Depletion of lactobacilli with antibiotic treatment partially restored C. albicans burdens in mice receiving sucrose.					
34399623	7	100	theme	due	1083:1085	arg1	diversity					1073:1081	a significantly reduced bacterial alpha diversity	1033:1081	a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci	1033:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	13	101	theme	local	2206:2210	arg1	factors					2223:2229	local modulating factors	2206:2229	local modulating factors (availability of sucrose as a carbon source)	2206:2274	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	2	102	theme	mucosal	279:285	arg1	bacteria					287:294	mucosal bacteria	279:294	mucosal bacteria	279:294	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	7	103	theme	bacterial	1057:1065	arg1	diversity					1073:1081	a significantly reduced bacterial alpha diversity	1033:1081	a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci	1033:1141	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	1	104	theme	prevalent	127:135	arg1	infection					142:150	the most prevalent oral infection	118:150	the most prevalent oral infection in immunocompromised patients	118:180	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	13	105	with	infection	2174:2182	arg1	albicans					2192:2199	C. albicans	2189:2199	C. albicans	2189:2199	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	5	106	theme	culture	771:777	arg1	methods					779:785	16S rRNA gene sequencing and culture methods	742:785	methods	779:785	We also analyzed the mucosal bacterial composition using 16S rRNA gene sequencing and culture methods.					
34399623	13	107	theme	factors	2223:2229	arg1	effects					2121:2127	the effects	2117:2127	the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence	2117:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	9	108	theme	SparCC	1331:1336	arg1	networks					1350:1357	SparCC correlation networks	1331:1357	SparCC correlation networks	1331:1357	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	8	109	theme	dietary	1160:1166	arg1	sucrose					1168:1174	dietary sucrose	1160:1174	dietary sucrose	1160:1174	When exposed to dietary sucrose, these mice had reduced C. albicans burdens and reduced bacterial alpha diversity, associated with an increase in the relative abundance of Lactobacillus.					
34399623	1	110	theme	immunocompromised	155:171	arg1	patients					173:180	immunocompromised patients	155:180	immunocompromised patients	155:180	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	10	111	theme	C.	1541:1542	arg1	burdens					1553:1559	C. albicans burdens	1541:1559	C. albicans burdens	1541:1559	Depletion of lactobacilli with antibiotic treatment partially restored C. albicans burdens in mice receiving sucrose.					
34399623	11	112	theme	in	1601:1602	arg1	experiments					1610:1620	coculture in vitro experiments	1591:1620	coculture in vitro experiments	1591:1620	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	5	113	theme	rRNA	746:749	arg1	sequencing					756:765	16S rRNA gene sequencing and culture methods	742:785	sequencing	756:765	We also analyzed the mucosal bacterial composition using 16S rRNA gene sequencing and culture methods.					
34399623	15	114	theme	Candida	2527:2533	arg1	virulence					2535:2543	Candida virulence	2527:2543	Candida virulence	2527:2543	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	9	115	from	Enterococcus	1427:1438	arg1	mice					1464:1467	all Candida-infected mice	1443:1467	all Candida-infected mice	1443:1467	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	7	116	theme	C.	991:992	arg1	infection					1003:1011	C. albicans infection	991:1011	C. albicans infection	991:1011	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	16	117	theme	Candida	2698:2704	arg1	pathogenesis					2706:2717	Candida pathogenesis	2698:2717	Candida pathogenesis	2698:2717	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	13	118	from	microbiome	2301:2310	arg1	virulence					2335:2343	fungal virulence	2328:2343	fungal virulence	2328:2343	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	14	119	theme	microbial	2383:2391	arg1	communities					2393:2403	endogenous microbial communities	2372:2403	endogenous microbial communities	2372:2403	We showed that changes in endogenous microbial communities in response to sucrose can lead to attenuation of fungal disease.					
34399623	9	120	from	Lactobacillus	1409:1421	arg1	mice					1464:1467	all Candida-infected mice	1443:1467	all Candida-infected mice	1443:1467	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	4	121	theme	cortisone	629:637	arg1	model					657:661	a mouse cortisone immunosuppression model	621:661	a mouse cortisone immunosuppression model amended with sucrose	621:682	C. albicans burdens and mucosal lesions were evaluated in a mouse cortisone immunosuppression model amended with sucrose.					
34399623	1	122	theme	Oropharyngeal	83:95	arg1	OPC					110:112	OPC	110:112	OPC	110:112	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	1	122	theme	Oropharyngeal	83:95	arg1	candidiasis					97:107	Oropharyngeal candidiasis	83:107	Oropharyngeal candidiasis (OPC)	83:113	Oropharyngeal candidiasis (OPC) is the most prevalent oral infection in immunocompromised patients, primarily associated with Candida albicans.					
34399623	13	123	with	factors	2223:2229	arg1	albicans					2192:2199	C. albicans	2189:2199	C. albicans	2189:2199	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
34399623	16	124	theme	conducive	2814:2822	arg1	microbiota					2803:2812	a mucosal bacterial microbiota	2783:2812	a mucosal bacterial microbiota conducive to virulence	2783:2835	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	0	125	from	Virulence	43:51	arg1	Candidiasis					70:80	Oropharyngeal Candidiasis	56:80	Oropharyngeal Candidiasis	56:80	Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis.					
34399623	11	126	dep	in	1601:1602	arg1	vitro					1604:1608	vitro	1604:1608	vitro	1604:1608	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	3	127	theme	mucosal	441:447	arg1	microbiota					459:468	the mucosal bacterial microbiota	437:468	the mucosal bacterial microbiota	437:468	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	3	128	from	changes	394:400	arg1	composition					422:432	composition	422:432	composition	422:432	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	3	128	from	changes	394:400	arg1	abundance					409:417	abundance	409:417	abundance	409:417	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	15	129	theme	synergistic	2619:2629	arg1	bacteria					2631:2638	potentially synergistic bacteria	2607:2638	potentially synergistic bacteria such as enterococci	2607:2658	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	15	129	theme	synergistic	2619:2629	arg1	enterococci					2648:2658	enterococci	2648:2658	enterococci	2648:2658	We also showed that Lactobacillus johnsonii may curtail Candida virulence both by inhibiting its growth and by inhibiting the growth of potentially synergistic bacteria such as enterococci.					
34399623	12	130	theme	sucrose-induced	1781:1795	arg1	result					1840:1845	a result	1838:1845	a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1838:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	130	theme	sucrose-induced	1781:1795	arg1	attenuation					1797:1807	the sucrose-induced attenuation	1777:1807	the sucrose-induced attenuation of C. albicans virulence	1777:1832	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	131	from	microbiome	1883:1892	arg1	result					1840:1845	a result	1838:1845	a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli	1838:1968	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	12	131	from	microbiome	1883:1892	arg1	attenuation					1797:1807	the sucrose-induced attenuation	1777:1807	the sucrose-induced attenuation of C. albicans virulence	1777:1832	These results support the hypothesis that the sucrose-induced attenuation of C. albicans virulence was a result of changes in the mucosal bacterial microbiome characterized by a reduction in enterococci and an increase in lactobacilli.					
34399623	4	132	theme	C.	563:564	arg1	burdens					575:581	C. albicans burdens	563:581	C. albicans burdens	563:581	C. albicans burdens and mucosal lesions were evaluated in a mouse cortisone immunosuppression model amended with sucrose.					
34399623	2	133	dep	commensal	334:342	arg1	pathogenic					347:356	pathogenic	347:356	pathogenic	347:356	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	2	133	dep	commensal	334:342	arg1	to					344:345	to	344:345	to	344:345	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	0	134	theme	Candida	26:32	arg1	albicans					34:41	Candida albicans	26:41	Candida albicans Virulence in Oropharyngeal Candidiasis	26:80	Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis.					
34399623	7	135	theme	immunocompromised	967:983	arg1	mice					985:988	immunocompromised mice	967:988	immunocompromised mice	967:988	In immunocompromised mice, C. albicans infection was associated with a significantly reduced bacterial alpha diversity due to an increase in the relative abundance of enterococci.					
34399623	14	136	theme	endogenous	2372:2381	arg1	communities					2393:2403	endogenous microbial communities	2372:2403	endogenous microbial communities	2372:2403	We showed that changes in endogenous microbial communities in response to sucrose can lead to attenuation of fungal disease.					
34399623	11	137	theme	isolates	1709:1716	arg1	growth					1677:1682	growth	1677:1682	growth of Enterococcus faecalis isolates and C. albicans	1677:1732	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	3	138	theme	microbiota	459:468	arg1	composition					422:432	composition	422:432	composition	422:432	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	3	138	theme	microbiota	459:468	arg1	abundance					409:417	abundance	409:417	abundance	409:417	In this work, we hypothesized that changes in the abundance or composition of the mucosal bacterial microbiota induced by dietary sucrose during the development of OPC can modulate C. albicans virulence.					
34399623	0	139	theme	Oropharyngeal	56:68	arg1	Candidiasis					70:80	Oropharyngeal Candidiasis	56:80	Oropharyngeal Candidiasis	56:80	Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis.					
34399623	4	140	dep	C.	563:564	arg1	albicans					566:573	albicans	566:573	albicans	566:573	C. albicans burdens and mucosal lesions were evaluated in a mouse cortisone immunosuppression model amended with sucrose.					
34399623	2	141	theme	Increasing	227:236	arg1	evidence					238:245	Increasing evidence	227:245	Increasing evidence	227:245	Increasing evidence points to a significant role of mucosal bacteria on the transition of C. albicans from commensal to pathogenic.					
34399623	11	142	theme	Enterococcus	1687:1698	arg1	isolates					1709:1716	Enterococcus faecalis isolates	1687:1716	Enterococcus faecalis isolates	1687:1716	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	9	143	theme	Candida-infected	1447:1462	arg1	mice					1464:1467	all Candida-infected mice	1443:1467	all Candida-infected mice	1443:1467	SparCC correlation networks showed a significant negative correlation between Lactobacillus and Enterococcus in all Candida-infected mice.					
34399623	6	144	theme	reduced	873:879	arg1	diversity					887:895	reduced alpha diversity	873:895	reduced alpha diversity	873:895	In immunocompetent mice, sucrose significantly increased total bacterial burdens and reduced alpha diversity, by increasing the relative abundance of mitis group streptococci.					
34399623	16	145	theme	host	2774:2777	arg1	contexts					2743:2750	the contexts	2739:2750	the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence	2739:2835	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	16	146	theme	microbiota	2803:2812	arg1	contexts					2743:2750	the contexts	2739:2750	the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence	2739:2835	Our results support the concept that Candida pathogenesis should be viewed in the contexts of both a susceptible host and a mucosal bacterial microbiota conducive to virulence.					
34399623	4	147	theme	mouse	623:627	arg1	model					657:661	a mouse cortisone immunosuppression model	621:661	a mouse cortisone immunosuppression model amended with sucrose	621:682	C. albicans burdens and mucosal lesions were evaluated in a mouse cortisone immunosuppression model amended with sucrose.					
34399623	11	148	theme	C.	1722:1723	arg1	albicans					1725:1732	C. albicans	1722:1732	C. albicans	1722:1732	In coculture in vitro experiments, mouse oral Lactobacillus johnsonii isolates inhibited growth of Enterococcus faecalis isolates and C. albicans.					
34399623	13	149	theme	mucosal	2283:2289	arg1	microbiome					2301:2310	the mucosal bacterial microbiome	2279:2310	the mucosal bacterial microbiome	2279:2310	IMPORTANCE By comparing Candida albicans virulence and the mucosal bacterial composition in a mouse oral infection model, we were able to dissect the effects of the host environment (immunosuppression), infection with C. albicans, and local modulating factors (availability of sucrose as a carbon source) on the mucosal bacterial microbiome and its role on fungal virulence.					
31924200	0	0	theme	containing-Lactobacillus	82:105	arg1	plantarum					107:115	synbiotic containing-Lactobacillus plantarum	72:115	synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats	72:166	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	8	1	theme	cortex	945:950	arg1	obtainment					916:925	the obtainment	912:925	the obtainment of the prefrontal cortex (PFC) and hippocampal samples	912:980	Subsequently, the obtainment of the prefrontal cortex (PFC) and hippocampal samples.					
31924200	4	2	theme	therapeutic and	561:575	arg1	effects					596:602	the therapeutic and neuropsychological effects	557:602	the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats	557:646	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
31924200	0	3	theme	synbiotic	72:80	arg1	plantarum					107:115	synbiotic containing-Lactobacillus plantarum	72:115	synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats	72:166	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	13	4	dep	CONCLUSION	1393:1402	arg1	confirm					1417:1423	confirm	1417:1423	confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic	1417:1509	CONCLUSION The findings confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic.					
31924200	9	5	theme	plantarum	1039:1047	arg1	administration					1018:1031	administration	1018:1031	administration of L. plantarum and inulin	1018:1058	RESULTS Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status.					
31924200	4	6	from	effects	596:602	arg1	rats					643:646	diabetic rats	634:646	diabetic rats	634:646	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
31924200	6	7	theme	fecal	788:792	arg1	samples					794:800	fecal samples	788:800	fecal samples	788:800	in the end, fecal samples were collected to evaluate the gut microbial composition.					
31924200	11	8	theme	strong	1204:1209	arg1	correlation					1211:1221	a strong correlation	1202:1221	a strong correlation between the gut microbiota changes and cognition responses	1202:1280	Notably, a strong correlation between the gut microbiota changes and cognition responses was observed.					
31924200	11	9	theme	cognition	1262:1270	arg1	responses					1272:1280	cognition responses	1262:1280	cognition responses	1262:1280	Notably, a strong correlation between the gut microbiota changes and cognition responses was observed.					
31924200	8	10	theme	hippocampal	962:972	arg1	samples					974:980	hippocampal samples	962:980	hippocampal samples	962:980	Subsequently, the obtainment of the prefrontal cortex (PFC) and hippocampal samples.					
31924200	9	11	theme	inulin	1053:1058	arg1	administration					1018:1031	administration	1018:1031	administration of L. plantarum and inulin	1018:1058	RESULTS Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status.					
31924200	4	12	theme	neuropsychological	577:594	arg1	effects					596:602	the therapeutic and neuropsychological effects	557:602	the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats	557:646	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
31924200	4	13	theme	inulin	624:629	arg1	effects					596:602	the therapeutic and neuropsychological effects	557:602	the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats	557:646	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
31924200	1	14	theme	gut	200:202	arg1	microbiota					204:213	gut microbiota	200:213	gut microbiota	200:213	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	0	15	theme	neuropsychological	120:137	arg1	performance					139:149	neuropsychological performance	120:149	neuropsychological performance of diabetic rats	120:166	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	14	16	theme	balanced	1569:1576	arg1	microbiome					1582:1591	balanced gut microbiome	1569:1591	balanced gut microbiome	1569:1591	Moreover, neuropsychological improvement associated with balanced gut microbiome.					
31924200	1	17	theme	microbiota	204:213	arg1	manipulation					184:195	The manipulation	180:195	The manipulation of gut microbiota as a target	180:225	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	0	18	theme	potential	4:12	arg1	effects					26:32	The potential therapeutic effects	0:32	The potential therapeutic effects of the gut	0:43	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	5	19	theme	diabetic	761:768	arg1	rats					770:773	diabetic rats	761:773	diabetic rats	761:773	METHODS Throughout the investigation, L. plantarum, inulin or their combination (synbiotic) was administered to diabetic rats.					
31924200	8	20	theme	samples	974:980	arg1	obtainment					916:925	the obtainment	912:925	the obtainment of the prefrontal cortex (PFC) and hippocampal samples	912:980	Subsequently, the obtainment of the prefrontal cortex (PFC) and hippocampal samples.					
31924200	12	21	theme	stress	1377:1382	arg1	markers					1384:1390	oxidative stress markers	1367:1390	oxidative stress markers	1367:1390	Interestingly, synbiotics intake exploited a rather powerful effect on oxidative stress markers.					
31924200	9	22	theme	gut	1074:1076	arg1	dysbiosis					1078:1086	gut dysbiosis	1074:1086	gut dysbiosis	1074:1086	RESULTS Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status.					
31924200	1	23	theme	diseases	282:289	arg1	mellitus					315:322	type 2 diabetes mellitus	299:322	type 2 diabetes mellitus (T2DM)	299:329	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	1	23	theme	diseases	282:289	arg1	number					272:277	a number	270:277	a number of diseases such as type 2 diabetes mellitus (T2DM)	270:329	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	12	24	theme	oxidative	1367:1375	arg1	markers					1384:1390	oxidative stress markers	1367:1390	oxidative stress markers	1367:1390	Interestingly, synbiotics intake exploited a rather powerful effect on oxidative stress markers.					
31924200	0	25	theme	diabetic	154:161	arg1	rats					163:166	diabetic rats	154:166	diabetic rats	154:166	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	9	26	dep	demonstrated	1000:1011	arg1	RESULTS					983:989	RESULTS	983:989	RESULTS	983:989	RESULTS Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status.					
31924200	1	27	dep	BACKGROUND	169:178	arg1	suggested					236:244	suggested	236:244	has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM)	227:329	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	3	28	theme	probiotics	442:451	arg1	impact					432:437	the impact	428:437	the impact of probiotics and prebiotics to improve T2DM complications	428:496	Furthermore, the impact of probiotics and prebiotics to improve T2DM complications are reported.					
31924200	13	29	theme	therapeutic	1452:1462	arg1	potential					1464:1472	a beneficial therapeutic potential	1439:1472	a beneficial therapeutic potential	1439:1472	CONCLUSION The findings confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic.					
31924200	9	30	theme	oxidative	1092:1100	arg1	status					1109:1114	oxidative stress status	1092:1114	oxidative stress status	1092:1114	RESULTS Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status.					
31924200	2	31	theme	brain	398:402	arg1	disorders					404:412	brain disorders	398:412	brain disorders	398:412	Conversely, T2DM is associated with complications such as gut and brain disorders.					
31924200	1	32	theme	type	299:302	arg1	T2DM					325:328	T2DM	325:328	T2DM	325:328	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	1	32	theme	type	299:302	arg1	mellitus					315:322	type 2 diabetes mellitus	299:322	type 2 diabetes mellitus (T2DM)	299:329	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	6	33	theme	microbial	837:845	arg1	composition					847:857	the gut microbial composition	829:857	the gut microbial composition	829:857	in the end, fecal samples were collected to evaluate the gut microbial composition.					
31924200	12	34	theme	powerful	1348:1355	arg1	effect					1357:1362	a rather powerful effect	1339:1362	a rather powerful effect on oxidative stress markers	1339:1390	Interestingly, synbiotics intake exploited a rather powerful effect on oxidative stress markers.					
31924200	9	35	theme	stress	1102:1107	arg1	status					1109:1114	oxidative stress status	1092:1114	oxidative stress status	1092:1114	RESULTS Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status.					
31924200	14	36	theme	gut	1578:1580	arg1	microbiome					1582:1591	balanced gut microbiome	1569:1591	balanced gut microbiome	1569:1591	Moreover, neuropsychological improvement associated with balanced gut microbiome.					
31924200	0	37	theme	rats	163:166	arg1	performance					139:149	neuropsychological performance	120:149	neuropsychological performance of diabetic rats	120:166	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	6	38	theme	gut	833:835	arg1	composition					847:857	the gut microbial composition	829:857	the gut microbial composition	829:857	in the end, fecal samples were collected to evaluate the gut microbial composition.					
31924200	4	39	theme	diabetic	634:641	arg1	rats					643:646	diabetic rats	634:646	diabetic rats	634:646	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
31924200	1	40	theme	diabetes	306:313	arg1	T2DM					325:328	T2DM	325:328	T2DM	325:328	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	1	40	theme	diabetes	306:313	arg1	mellitus					315:322	type 2 diabetes mellitus	299:322	type 2 diabetes mellitus (T2DM)	299:329	BACKGROUND The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM).					
31924200	11	41	theme	microbiota	1239:1248	arg1	changes					1250:1256	the gut microbiota changes	1231:1256	the gut microbiota changes	1231:1256	Notably, a strong correlation between the gut microbiota changes and cognition responses was observed.					
31924200	13	42	theme	beneficial	1441:1450	arg1	potential					1464:1472	a beneficial therapeutic potential	1439:1472	a beneficial therapeutic potential	1439:1472	CONCLUSION The findings confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic.					
31924200	0	43	theme	gut	41:43	arg1	effects					26:32	The potential therapeutic effects	0:32	The potential therapeutic effects of the gut	0:43	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	4	44	theme	present	522:528	arg1	study					530:534	the present study	518:534	the present study	518:534	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
31924200	3	45	theme	T2DM	479:482	arg1	complications					484:496	T2DM complications	479:496	T2DM complications	479:496	Furthermore, the impact of probiotics and prebiotics to improve T2DM complications are reported.					
31924200	14	46	theme	neuropsychological	1522:1539	arg1	improvement					1541:1551	neuropsychological improvement	1522:1551	neuropsychological improvement	1522:1551	Moreover, neuropsychological improvement associated with balanced gut microbiome.					
31924200	3	47	theme	prebiotics	457:466	arg1	impact					432:437	the impact	428:437	the impact of probiotics and prebiotics to improve T2DM complications	428:496	Furthermore, the impact of probiotics and prebiotics to improve T2DM complications are reported.					
31924200	12	48	from	effect	1357:1362	arg1	markers					1384:1390	oxidative stress markers	1367:1390	oxidative stress markers	1367:1390	Interestingly, synbiotics intake exploited a rather powerful effect on oxidative stress markers.					
31924200	10	49	theme	signaling	1174:1182	arg1	pathway					1184:1190	BDNF/TrkB signaling pathway	1164:1190	BDNF/TrkB signaling pathway	1164:1190	In addition, it could ameliorate serotonin and BDNF/TrkB signaling pathway.					
31924200	11	50	theme	gut	1235:1237	arg1	changes					1250:1256	the gut microbiota changes	1231:1256	the gut microbiota changes	1231:1256	Notably, a strong correlation between the gut microbiota changes and cognition responses was observed.					
31924200	12	51	theme	synbiotics	1311:1320	arg1	intake					1322:1327	synbiotics intake	1311:1327	synbiotics intake	1311:1327	Interestingly, synbiotics intake exploited a rather powerful effect on oxidative stress markers.					
31924200	8	52	theme	prefrontal	934:943	arg1	PFC					953:955	PFC	953:955	PFC	953:955	Subsequently, the obtainment of the prefrontal cortex (PFC) and hippocampal samples.					
31924200	8	52	theme	prefrontal	934:943	arg1	cortex					945:950	the prefrontal cortex	930:950	the prefrontal cortex (PFC)	930:956	Subsequently, the obtainment of the prefrontal cortex (PFC) and hippocampal samples.					
31924200	10	53	theme	BDNF/TrkB	1164:1172	arg1	pathway					1184:1190	BDNF/TrkB signaling pathway	1164:1190	BDNF/TrkB signaling pathway	1164:1190	In addition, it could ameliorate serotonin and BDNF/TrkB signaling pathway.					
31924200	0	54	from	plantarum	107:115	arg1	performance					139:149	neuropsychological performance	120:149	neuropsychological performance of diabetic rats	120:166	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	0	55	dep	potential	4:12	arg1	therapeutic					14:24	therapeutic	14:24	therapeutic	14:24	The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats.					
31924200	13	56	theme	supplements	1477:1487	arg1	potential					1464:1472	a beneficial therapeutic potential	1439:1472	a beneficial therapeutic potential	1439:1472	CONCLUSION The findings confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic.					
31924200	13	57	theme	symbiotic	1501:1509	arg1	potential					1464:1472	a beneficial therapeutic potential	1439:1472	a beneficial therapeutic potential	1439:1472	CONCLUSION The findings confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic.					
31924200	7	58	theme	behavioral	865:874	arg1	tests					876:880	Then behavioral tests	860:880	Then behavioral tests	860:880	Then behavioral tests were conducted.					
31924200	4	59	theme	plantarum	610:618	arg1	effects					596:602	the therapeutic and neuropsychological effects	557:602	the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats	557:646	Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats.					
34425121	4	0	theme	reducing	652:659	arg1	agent					677:681	reducing and stabilizing agent	652:681	agent	677:681	Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent.					
34425121	7	1	theme	free	959:962	arg1	cryogel					964:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	1	2	theme	nanocomposite	182:194	arg1	cryogel					196:202	a new 3D nanocomposite cryogel	173:202	a new 3D nanocomposite cryogel	173:202	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	12	3	theme	new	1463:1465	arg1	cryogel					1480:1486	the new 3D composite cryogel	1459:1486	the new 3D composite cryogel	1459:1486	Overall, the new 3D composite cryogel can efficiently remove dyes and bacteria from wastewater.					
34425121	9	4	theme	cryogel	1199:1205	arg1	1.5 g/L					1184:1190	1.5 g/L	1184:1190	1.5 g/L of the cryogel at pH 1	1184:1213	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	6	5	theme	Cs	863:864	arg1	mixture					852:858	a mixture	850:858	a mixture of Cs and NFC	850:872	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	5	6	theme	Ag	697:698	arg1	free					715:718	free	715:718	free	715:718	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	6	theme	Ag	697:698	arg1	particles					700:708	The prepared Ag particles	684:708	The prepared Ag particles	684:708	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	4	7	dep	the	608:610	arg1	aid					612:614	aid	612:614	aid	612:614	Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent.					
34425121	4	8	theme	Ag	574:575	arg1	NPs					577:579	Ag NPs	574:579	Ag NPs	574:579	Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent.					
34425121	11	9	theme	prepared	1343:1350	arg1	cryogels					1352:1359	All prepared cryogels	1339:1359	All prepared cryogels	1339:1359	All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs.					
34425121	11	10	contain	have	1361:1364	arg1	cryogels					1352:1359	All prepared cryogels	1339:1359	All prepared cryogels	1339:1359	All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs.					
34425121	11	10	contain	have	1361:1364	arg2	properties					1388:1397	notable antibacterial properties	1366:1397	notable antibacterial properties that were significantly improved by adding Ag NPs	1366:1447	All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs.					
34425121	3	11	with	cryogel	487:493	arg1	properties					511:520	adsorption properties	500:520	adsorption properties	500:520	Both Cs and NFC endow the cryogel with adsorption properties while Ag NPs enhances its antibacterial properties.					
34425121	10	12	theme	Freundlich	1303:1312	arg1	equations					1314:1322	the pseudo-first-order and Freundlich equations	1276:1322	equations	1314:1322	The adsorption kinetics and isotherm were best described by the pseudo-first-order and Freundlich equations, respectively.					
34425121	4	13	theme	stabilizing	665:675	arg1	agent					677:681	reducing and stabilizing agent	652:681	agent	677:681	Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent.					
34425121	11	14	theme	notable	1366:1372	arg1	properties					1388:1397	notable antibacterial properties	1366:1397	notable antibacterial properties that were significantly improved by adding Ag NPs	1366:1447	All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs.					
34425121	12	15	theme	3D	1467:1468	arg1	cryogel					1480:1486	the new 3D composite cryogel	1459:1486	the new 3D composite cryogel	1459:1486	Overall, the new 3D composite cryogel can efficiently remove dyes and bacteria from wastewater.					
34425121	7	16	theme	surface	1018:1024	arg1	area					1026:1029	large surface area	1012:1029	large surface area	1012:1029	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	11	17	theme	antibacterial	1374:1386	arg1	properties					1388:1397	notable antibacterial properties	1366:1397	notable antibacterial properties that were significantly improved by adding Ag NPs	1366:1447	All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs.					
34425121	0	18	theme	antibacterial	132:144	arg1	characteristics					146:160	antibacterial characteristics	132:160	antibacterial characteristics	132:160	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics.					
34425121	2	19	theme	mechanical	414:423	arg1	properties					425:434	the mechanical properties	410:434	the mechanical properties of the produced cryogel	410:458	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	7	20	contain	has	972:974	arg1	cryogel					964:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	7	20	contain	has	972:974	arg2	surface					999:1005	highly porosity smooth surface	976:1005	highly porosity smooth surface	976:1005	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	4	21	theme	microwave	619:627	arg1	radiation					629:637	microwave radiation	619:637	microwave radiation	619:637	Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent.					
34425121	0	22	theme	3D	16:17	arg1	nanoparticles					68:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics.					
34425121	6	23	theme	porous	912:917	arg1	cryogel					919:925	porous cryogel	912:925	porous cryogel (3D microstructure)	912:945	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	6	23	theme	porous	912:917	arg1	microstructure					931:944	3D microstructure	928:944	3D microstructure	928:944	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	9	24	theme	Optimum	1103:1109	arg1	adsorption					1111:1120	Optimum adsorption	1103:1120	Optimum adsorption of reactive blue 49	1103:1140	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	0	25	theme	Multifunctional	0:14	arg1	nanoparticles					68:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics.					
34425121	1	26	theme	silver	289:294	arg1	nanoparticles					296:308	silver nanoparticles	289:308	silver nanoparticles (Ag NPs)	289:317	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	26	theme	silver	289:294	arg1	NPs					314:316	Ag NPs	311:316	Ag NPs	311:316	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	7	27	theme	large	1012:1016	arg1	area					1026:1029	large surface area	1012:1029	large surface area	1012:1029	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	9	28	theme	49	1139:1140	arg1	adsorption					1111:1120	Optimum adsorption	1103:1120	Optimum adsorption of reactive blue 49	1103:1140	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	9	29	from	pH 1	1210:1213	arg1	1.5 g/L					1184:1190	1.5 g/L	1184:1190	1.5 g/L of the cryogel at pH 1	1184:1213	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	9	29	from	pH 1	1210:1213	arg1	cryogel					1199:1205	the cryogel	1195:1205	the cryogel at pH 1	1195:1213	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	0	30	theme	starch/nanofibrillated	28:49	arg1	nanoparticles					68:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics.					
34425121	7	31	theme	smooth	992:997	arg1	surface					999:1005	highly porosity smooth surface	976:1005	highly porosity smooth surface	976:1005	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	3	32	theme	Ag	528:529	arg1	NPs					531:533	Ag NPs	528:533	Ag NPs	528:533	Both Cs and NFC endow the cryogel with adsorption properties while Ag NPs enhances its antibacterial properties.					
34425121	10	33	theme	adsorption	1220:1229	arg1	kinetics					1231:1238	The adsorption kinetics	1216:1238	The adsorption kinetics	1216:1238	The adsorption kinetics and isotherm were best described by the pseudo-first-order and Freundlich equations, respectively.					
34425121	1	34	theme	cationic	231:238	arg1	starch					240:245	cationic starch	231:245	cationic starch (Cs)	231:250	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	34	theme	cationic	231:238	arg1	Cs					248:249	Cs	248:249	Cs	248:249	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	0	35	theme	cationic	19:26	arg1	nanoparticles					68:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics.					
34425121	5	36	dep	sizes	739:743	arg1	<10 nm					745:750	<10 nm	745:750	<10 nm	745:750	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	36	dep	sizes	739:743	arg1	stability					761:769	good stability	756:769	good stability	756:769	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	36	dep	sizes	739:743	arg1	sizes					739:743	sizes <10 nm and good stability	739:769	sizes <10 nm and good stability in solution	739:781	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	1	37	theme	starch	240:245	arg1	advantages					217:226	the advantages	213:226	the advantages of cationic starch (Cs)	213:250	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	37	theme	starch	240:245	arg1	NFC					280:282	NFC	280:282	NFC	280:282	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	37	theme	starch	240:245	arg1	cellulose					269:277	nanofibrillated cellulose	253:277	nanofibrillated cellulose (NFC)	253:283	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	37	theme	starch	240:245	arg1	nanoparticles					296:308	silver nanoparticles	289:308	silver nanoparticles (Ag NPs)	289:317	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	37	theme	starch	240:245	arg1	NPs					314:316	Ag NPs	311:316	Ag NPs	311:316	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	2	38	theme	cryogel	452:458	arg1	properties					425:434	the mechanical properties	410:434	the mechanical properties of the produced cryogel	410:458	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	1	39	theme	Ag	311:312	arg1	nanoparticles					296:308	silver nanoparticles	289:308	silver nanoparticles (Ag NPs)	289:317	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	39	theme	Ag	311:312	arg1	NPs					314:316	Ag NPs	311:316	Ag NPs	311:316	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	6	40	theme	NPs	832:834	arg1	concentrations					798:811	Two different concentrations	784:811	Two different concentrations of the prepared Ag NPs	784:834	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	2	41	theme	filling	385:391	arg1	NFC					367:369	NFC	367:369	NFC	367:369	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	2	41	theme	filling	385:391	arg1	agent					393:397	a filling agent	383:397	a filling agent to enhance the mechanical properties of the produced cryogel	383:458	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	2	42	theme	produced	443:450	arg1	cryogel					452:458	the produced cryogel	439:458	the produced cryogel	439:458	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	6	43	theme	freeze	891:896	arg1	drying					898:903	freeze drying	891:903	freeze drying	891:903	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	0	44	theme	cellulose/silver	51:66	arg1	nanoparticles					68:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles	0:80	Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics.					
34425121	6	45	theme	Ag	829:830	arg1	NPs					832:834	the prepared Ag NPs	816:834	the prepared Ag NPs	816:834	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	8	46	theme	3D	1089:1090	arg1	roughness					1092:1100	the 3D roughness	1085:1100	the 3D roughness	1085:1100	Adding Ag NPs decreased these features and increased the 3D roughness.					
34425121	5	47	theme	good	756:759	arg1	stability					761:769	good stability	756:769	good stability	756:769	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	47	theme	good	756:759	arg1	sizes					739:743	sizes <10 nm and good stability	739:769	sizes <10 nm and good stability in solution	739:781	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	6	48	theme	prepared	820:827	arg1	NPs					832:834	the prepared Ag NPs	816:834	the prepared Ag NPs	816:834	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	3	49	theme	antibacterial	548:560	arg1	properties					562:571	its antibacterial properties	544:571	its antibacterial properties	544:571	Both Cs and NFC endow the cryogel with adsorption properties while Ag NPs enhances its antibacterial properties.					
34425121	7	50	theme	Ag	952:953	arg1	cryogel					964:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	7	51	theme	porosity	983:990	arg1	surface					999:1005	highly porosity smooth surface	976:1005	highly porosity smooth surface	976:1005	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	5	52	theme	prepared	688:695	arg1	free					715:718	free	715:718	free	715:718	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	52	theme	prepared	688:695	arg1	particles					700:708	The prepared Ag particles	684:708	The prepared Ag particles	684:708	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	9	53	theme	contact	1171:1177	arg1	30 min					1161:1166	30 min	1161:1166	30 min of contact with 1.5 g/L of the cryogel at pH 1	1161:1213	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	5	54	from	sizes	739:743	arg1	solution					774:781	solution	774:781	solution	774:781	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	2	55	theme	main	331:334	arg1	component					336:344	the main component	327:344	the main component of the cryogel	327:359	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	2	55	theme	main	331:334	arg1	Cs					320:321	Cs	320:321	Cs	320:321	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	7	56	theme	NPs	955:957	arg1	cryogel					964:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel	948:970	The Ag NPs free cryogel has highly porosity smooth surface with large surface area.					
34425121	4	57	dep	green	585:589	arg1	synthesized					591:601	synthesized	591:601	was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent	581:681	Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent.					
34425121	12	58	theme	composite	1470:1478	arg1	cryogel					1480:1486	the new 3D composite cryogel	1459:1486	the new 3D composite cryogel	1459:1486	Overall, the new 3D composite cryogel can efficiently remove dyes and bacteria from wastewater.					
34425121	8	59	theme	Ag	1039:1040	arg1	NPs					1042:1044	Ag NPs	1039:1044	Ag NPs	1039:1044	Adding Ag NPs decreased these features and increased the 3D roughness.					
34425121	11	60	theme	Ag	1442:1443	arg1	NPs					1445:1447	Ag NPs	1442:1447	Ag NPs	1442:1447	All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs.					
34425121	3	61	theme	adsorption	500:509	arg1	properties					511:520	adsorption properties	500:520	adsorption properties	500:520	Both Cs and NFC endow the cryogel with adsorption properties while Ag NPs enhances its antibacterial properties.					
34425121	10	62	theme	pseudo-first-order	1280:1297	arg1	equations					1314:1322	the pseudo-first-order and Freundlich equations	1276:1322	equations	1314:1322	The adsorption kinetics and isotherm were best described by the pseudo-first-order and Freundlich equations, respectively.					
34425121	1	63	theme	3D	179:180	arg1	cryogel					196:202	a new 3D nanocomposite cryogel	173:202	a new 3D nanocomposite cryogel	173:202	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	9	64	with	contact	1171:1177	arg1	1.5 g/L					1184:1190	1.5 g/L	1184:1190	1.5 g/L of the cryogel at pH 1	1184:1213	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	9	65	from	1.5 g/L	1184:1190	arg1	pH 1					1210:1213	pH 1	1210:1213	pH 1	1210:1213	Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1.					
34425121	6	66	theme	different	788:796	arg1	concentrations					798:811	Two different concentrations	784:811	Two different concentrations of the prepared Ag NPs	784:834	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	1	67	theme	new	175:177	arg1	cryogel					196:202	a new 3D nanocomposite cryogel	173:202	a new 3D nanocomposite cryogel	173:202	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	68	theme	nanofibrillated	253:267	arg1	NFC					280:282	NFC	280:282	NFC	280:282	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	1	68	theme	nanofibrillated	253:267	arg1	cellulose					269:277	nanofibrillated cellulose	253:277	nanofibrillated cellulose (NFC)	253:283	We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs).					
34425121	5	69	theme	impurities	723:732	arg1	free					715:718	free	715:718	free	715:718	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	69	theme	impurities	723:732	arg1	particles					700:708	The prepared Ag particles	684:708	The prepared Ag particles	684:708	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	6	70	theme	NFC	870:872	arg1	mixture					852:858	a mixture	850:858	a mixture of Cs and NFC	850:872	Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure).					
34425121	5	71	with	impurities	723:732	arg1	<10 nm					745:750	<10 nm	745:750	<10 nm	745:750	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	71	with	impurities	723:732	arg1	stability					761:769	good stability	756:769	good stability	756:769	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	5	71	with	impurities	723:732	arg1	sizes					739:743	sizes <10 nm and good stability	739:769	sizes <10 nm and good stability in solution	739:781	The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution.					
34425121	2	72	used	used	375:378	arg2	NFC					367:369	NFC	367:369	NFC	367:369	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	2	72	used	used	375:378	arg2	agent					393:397	a filling agent	383:397	a filling agent to enhance the mechanical properties of the produced cryogel	383:458	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	2	73	theme	cryogel	353:359	arg1	component					336:344	the main component	327:344	the main component of the cryogel	327:359	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
34425121	2	73	theme	cryogel	353:359	arg1	Cs					320:321	Cs	320:321	Cs	320:321	Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel.					
33026342	5	0	theme	tissue-basement	848:862	arg1	membranes					864:872	the tissue-basement membranes	844:872	the tissue-basement membranes	844:872	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	4	1	theme	hematopoietic	753:765	arg1	environment					767:777	their natural hematopoietic environment	739:777	their natural hematopoietic environment	739:777	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	6	2	theme	hematopoietic	1158:1170	arg1	sites					1172:1176	hematopoietic sites	1158:1176	hematopoietic sites in Drosophila	1158:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	5	3	theme	cell-tissue	997:1007	arg1	association					1009:1019	immune cell-tissue association	990:1019	immune cell-tissue association	990:1019	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	0	4	theme	compartments	74:85	arg1	formation					47:55	the formation	43:55	the formation of hematopoietic compartments	43:85	Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments.					
33026342	3	5	dep	Drosophila	427:436	arg1	melanogaster					438:449	melanogaster	438:449	melanogaster	438:449	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	1	6	theme	nurture	182:188	arg1	precursors					204:213	nurture hematopoietic precursors	182:213	nurture hematopoietic precursors	182:213	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	2	7	with	interactions	334:345	arg1	microenvironments					366:382	hematopoietic microenvironments	352:382	hematopoietic microenvironments	352:382	The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood.					
33026342	5	8	theme	Collagen	802:809	arg1	Multiplexin					829:839	the Collagen XV/XVIII ortholog Multiplexin	798:839	the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes	798:872	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	8	theme	Collagen	802:809	arg1	necessary					955:963	necessary	955:963	necessary	955:963	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	9	theme	immune	990:995	arg1	association					1009:1019	immune cell-tissue association	990:1019	immune cell-tissue association	990:1019	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	10	theme	XV/XVIII	811:818	arg1	Multiplexin					829:839	the Collagen XV/XVIII ortholog Multiplexin	798:839	the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes	798:872	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	10	theme	XV/XVIII	811:818	arg1	necessary					955:963	necessary	955:963	necessary	955:963	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	3	11	theme	hematopoietic	536:548	arg1	compartments					550:561	hematopoietic compartments	536:561	hematopoietic compartments	536:561	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	5	12	theme	ortholog	820:827	arg1	Multiplexin					829:839	the Collagen XV/XVIII ortholog Multiplexin	798:839	the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes	798:872	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	12	theme	ortholog	820:827	arg1	necessary					955:963	necessary	955:963	necessary	955:963	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	4	13	theme	tissue	690:695	arg1	surfaces					697:704	tissue surfaces	690:704	tissue surfaces where they function similarly to their natural hematopoietic environment	690:777	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	1	14	theme	hematopoietic	190:202	arg1	precursors					204:213	nurture hematopoietic precursors	182:213	nurture hematopoietic precursors	182:213	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	6	15	theme	integral	1093:1100	arg1	part					1102:1105	an integral part	1090:1105	an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila	1090:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	6	15	theme	integral	1093:1100	arg1	cooperation					1050:1060	the cooperation	1046:1060	the cooperation of Multiplexin and Eater	1046:1085	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	5	16	from	Eater	904:908	arg1	hemocytes					917:925	the hemocytes	913:925	the hemocytes	913:925	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	16	from	Eater	904:908	arg1	membranes					864:872	the tissue-basement membranes	844:872	the tissue-basement membranes	844:872	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	2	17	theme	cell	312:315	arg1	homing					317:322	blood cell homing	306:322	blood cell homing	306:322	The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood.					
33026342	3	18	theme	membrane	495:502	arg1	composition					504:514	basement membrane composition	486:514	basement membrane composition	486:514	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	1	19	theme	Blood	88:92	arg1	development					94:104	Blood development	88:104	Blood development in multicellular organisms	88:131	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	6	20	theme	Eater	1081:1085	arg1	cooperation					1050:1060	the cooperation	1046:1060	the cooperation of Multiplexin and Eater	1046:1085	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	6	20	theme	Eater	1081:1085	arg1	part					1102:1105	an integral part	1090:1105	an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila	1090:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	6	21	from	sites	1172:1176	arg1	Drosophila					1181:1190	Drosophila	1181:1190	Drosophila	1181:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	2	22	theme	hematopoietic	352:364	arg1	microenvironments					366:382	hematopoietic microenvironments	352:382	hematopoietic microenvironments	352:382	The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood.					
33026342	3	23	theme	Drosophila	427:436	arg1	model					451:455	the Drosophila melanogaster model	423:455	the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments	423:561	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	4	24	theme	blood	639:643	arg1	cells					645:649	the fly blood cells	631:649	the fly blood cells known as hemocytes	631:668	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	1	25	theme	multicellular	109:121	arg1	organisms					123:131	multicellular organisms	109:131	multicellular organisms	109:131	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	1	26	theme	self-renewal	233:244	arg1	proliferation					247:259	proliferation	247:259	proliferation	247:259	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	4	27	theme	fly	635:637	arg1	cells					645:649	the fly blood cells	631:649	the fly blood cells known as hemocytes	631:668	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	5	28	from	Multiplexin	829:839	arg1	hemocytes					917:925	the hemocytes	913:925	the hemocytes	913:925	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	5	28	from	Multiplexin	829:839	arg1	membranes					864:872	the tissue-basement membranes	844:872	the tissue-basement membranes	844:872	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	3	29	theme	pivotal	469:475	arg1	role					477:480	a pivotal role	467:480	a pivotal role for basement membrane composition in the formation of hematopoietic compartments	467:561	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	2	30	with	mechanisms	287:296	arg1	microenvironments					366:382	hematopoietic microenvironments	352:382	hematopoietic microenvironments	352:382	The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood.					
33026342	5	31	theme	phagocytosis	882:893	arg1	Eater					904:908	the phagocytosis receptor Eater	878:908	the phagocytosis receptor Eater on the hemocytes	878:925	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	4	32	theme	extracellular	598:610	arg1	components					619:628	extracellular matrix components	598:628	extracellular matrix components	598:628	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	3	33	theme	basement	486:493	arg1	composition					504:514	basement membrane composition	486:514	basement membrane composition	486:514	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	3	34	theme	compartments	550:561	arg1	formation					523:531	the formation	519:531	the formation of hematopoietic compartments	519:561	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	5	35	theme	receptor	895:902	arg1	Eater					904:908	the phagocytosis receptor Eater	878:908	the phagocytosis receptor Eater on the hemocytes	878:925	We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association.					
33026342	4	36	theme	matrix	612:617	arg1	components					619:628	extracellular matrix components	598:628	extracellular matrix components	598:628	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	6	37	theme	Multiplexin	1065:1075	arg1	cooperation					1050:1060	the cooperation	1046:1060	the cooperation of Multiplexin and Eater	1046:1085	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	6	37	theme	Multiplexin	1065:1075	arg1	part					1102:1105	an integral part	1090:1105	an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila	1090:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	2	38	theme	blood	306:310	arg1	homing					317:322	blood cell homing	306:322	blood cell homing	306:322	The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood.					
33026342	3	39	from	role	477:480	arg1	formation					523:531	the formation	519:531	the formation of hematopoietic compartments	519:561	Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments.					
33026342	1	40	theme	specific	143:150	arg1	microenvironments					159:175	specific tissue microenvironments	143:175	specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation	143:280	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	0	41	theme	hematopoietic	60:72	arg1	compartments					74:85	hematopoietic compartments	60:85	hematopoietic compartments	60:85	Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments.					
33026342	1	42	theme	tissue	152:157	arg1	microenvironments					159:175	specific tissue microenvironments	143:175	specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation	143:280	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	1	43	from	development	94:104	arg1	organisms					123:131	multicellular organisms	109:131	multicellular organisms	109:131	Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation.					
33026342	6	44	theme	mechanism	1119:1127	arg1	part					1102:1105	an integral part	1090:1105	an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila	1090:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	6	44	theme	mechanism	1119:1127	arg1	cooperation					1050:1060	the cooperation	1046:1060	the cooperation of Multiplexin and Eater	1046:1085	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
33026342	4	45	theme	natural	745:751	arg1	environment					767:777	their natural hematopoietic environment	739:777	their natural hematopoietic environment	739:777	We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment.					
33026342	6	46	theme	homing	1112:1117	arg1	mechanism					1119:1127	a homing mechanism	1110:1127	a homing mechanism that specifies and maintains hematopoietic sites in Drosophila	1110:1190	These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila.					
34197112	2	0	theme	β-glucan	433:440	arg1	relationship					485:496	the potential relationship	471:496	the potential relationship between gut microbiota and its activity	471:536	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	2	0	theme	β-glucan	433:440	arg1	effects					422:428	the effects	418:428	the effects of β-glucan on MS and gut microbiota	418:465	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	3	1	theme	pro-inflammatory	683:698	arg1	cytokines					700:708	pro-inflammatory cytokines	683:708	pro-inflammatory cytokines in plasma including IL-6 and IL-1β	683:743	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	3	1	theme	pro-inflammatory	683:698	arg1	IL-1β					739:743	IL-1β	739:743	IL-1β	739:743	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	3	1	theme	pro-inflammatory	683:698	arg1	IL-6					730:733	IL-6	730:733	IL-6	730:733	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	3	2	theme	β-glucan	613:620	arg1	treatment					594:602	the treatment	590:602	the treatment of yeast β-glucan	590:620	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	0	3	from	Microbiota	159:168	arg1	Proliferation					105:117	Inhibited Proliferation	95:117	Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota	95:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	0	4	from	Improvement	0:10	arg1	Mice					59:62	High-Fat Diet-Induced Mice	37:62	High-Fat Diet-Induced Mice	37:62	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	4	5	theme	microbiota	840:849	arg1	composition					809:819	composition	809:819	composition	809:819	Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota.					
34197112	4	5	theme	microbiota	840:849	arg1	diversity					795:803	diversity	795:803	diversity	795:803	Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota.					
34197112	1	6	attach	linked	273:278	arg2	prevention					202:211	prevention	202:211	prevention of metabolic syndrome (MS) by dietary fibers	202:256	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	1	6	attach	linked	273:278	arg1	microbiota					287:296	gut microbiota	283:296	gut microbiota	283:296	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	4	7	dep	diversity	795:803	arg1	the					791:793	the	791:793	the	791:793	Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota.					
34197112	4	8	theme	gut	836:838	arg1	microbiota					840:849	HFD-induced gut microbiota	824:849	HFD-induced gut microbiota	824:849	Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota.					
34197112	5	9	theme	positive	939:946	arg1	correlation					948:958	significant positive correlation	927:958	significant positive correlation with metabolic changes	927:981	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	5	10	theme	gut	1184:1186	arg1	microbiota					1188:1197	gut microbiota	1184:1197	gut microbiota	1184:1197	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	2	11	theme	gut	452:454	arg1	microbiota					456:465	gut microbiota	452:465	gut microbiota	452:465	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	5	12	theme	relative	866:873	arg1	abundances					875:884	the relative abundances	862:884	the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes,	862:982	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	4	13	theme	HFD-induced	824:834	arg1	microbiota					840:849	HFD-induced gut microbiota	824:849	HFD-induced gut microbiota	824:849	Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota.					
34197112	0	14	theme	Inhibited	95:103	arg1	Proliferation					105:117	Inhibited Proliferation	95:117	Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota	95:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	2	15	from	effects	422:428	arg1	MS					445:446	MS	445:446	MS	445:446	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	2	15	from	effects	422:428	arg1	microbiota					456:465	gut microbiota	452:465	gut microbiota	452:465	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	5	16	with	correlation	948:958	arg1	changes					975:981	metabolic changes	965:981	metabolic changes	965:981	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	3	17	theme	HFD-induced	654:664	arg1	MS.					666:668	the HFD-induced MS.	650:668	the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased	650:758	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	3	18	from	cytokines	700:708	arg1	plasma					713:718	plasma	713:718	plasma	713:718	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	1	19	theme	growing	180:186	arg1	evidence					188:195	growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota	180:296	growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota	180:296	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	2	20	theme	potential	475:483	arg1	relationship					485:496	the potential relationship	471:496	the potential relationship between gut microbiota and its activity	471:536	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	5	21	theme	Lactococcus	907:917	arg1	abundances					875:884	the relative abundances	862:884	the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes,	862:982	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	5	22	contain	having	920:925	arg2	correlation					948:958	significant positive correlation	927:958	significant positive correlation with metabolic changes	927:981	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	5	22	contain	having	920:925	arg1	Lactobacillus					889:901	Lactobacillus	889:901	Lactobacillus	889:901	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	2	23	theme	high-fat	340:347	arg1	HFD					355:357	HFD	355:357	HFD	355:357	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	2	23	theme	high-fat	340:347	arg1	diet					349:352	a high-fat diet	338:352	a high-fat diet (HFD)	338:358	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	0	24	theme	Syndrome	25:32	arg1	Improvement					0:10	Improvement	0:10	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan	0:80	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	0	25	theme	Lactobacillus	122:134	arg1	Proliferation					105:117	Inhibited Proliferation	95:117	Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota	95:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	5	26	theme	Lactobacillus	889:901	arg1	abundances					875:884	the relative abundances	862:884	the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes,	862:982	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	0	27	theme	Metabolic	15:23	arg1	Syndrome					25:32	Metabolic Syndrome	15:32	Metabolic Syndrome	15:32	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	5	28	theme	critical	1031:1038	arg1	role					1040:1043	a critical role	1029:1043	a critical role	1029:1043	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	0	29	theme	High-Fat	37:44	arg1	Mice					59:62	High-Fat Diet-Induced Mice	37:62	High-Fat Diet-Induced Mice	37:62	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	1	30	theme	gut	283:285	arg1	microbiota					287:296	gut microbiota	283:296	gut microbiota	283:296	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	0	31	theme	Lactococcus	140:150	arg1	Proliferation					105:117	Inhibited Proliferation	95:117	Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota	95:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	1	32	theme	dietary	243:249	arg1	fibers					251:256	dietary fibers	243:256	dietary fibers	243:256	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	2	33	theme	yeast	384:388	arg1	β-glucan					390:397	yeast β-glucan	384:397	yeast β-glucan	384:397	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	1	34	theme	metabolic	216:224	arg1	MS					236:237	MS	236:237	MS	236:237	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	1	34	theme	metabolic	216:224	arg1	syndrome					226:233	metabolic syndrome	216:233	metabolic syndrome (MS)	216:238	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	5	35	theme	promising	1114:1122	arg1	application					1124:1134	promising application	1114:1134	promising application	1114:1134	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	5	35	theme	promising	1114:1122	arg1	prebiotic					1141:1149	a prebiotic	1139:1149	a prebiotic for preventing MS and regulating gut microbiota	1139:1197	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	0	36	theme	Gut	155:157	arg1	Microbiota					159:168	Gut Microbiota	155:168	Gut Microbiota	155:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	5	37	theme	yeast	1093:1097	arg1	β-glucan					1099:1106	yeast β-glucan	1093:1106	yeast β-glucan	1093:1106	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	0	38	theme	Diet-Induced	46:57	arg1	Mice					59:62	High-Fat Diet-Induced Mice	37:62	High-Fat Diet-Induced Mice	37:62	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	3	39	dep	MS.	666:668	arg1	decreased					750:758	decreased	750:758	were decreased	745:758	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	0	40	from	Proliferation	105:117	arg1	Microbiota					159:168	Gut Microbiota	155:168	Gut Microbiota	155:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	3	41	theme	yeast	607:611	arg1	β-glucan					613:620	yeast β-glucan	607:620	yeast β-glucan	607:620	After intervention for 10 weeks, it was found that the treatment of yeast β-glucan could significantly improve the HFD-induced MS. Furthermore, pro-inflammatory cytokines in plasma including IL-6 and IL-1β were decreased.					
34197112	5	42	theme	metabolic	965:973	arg1	changes					975:981	metabolic changes	965:981	metabolic changes	965:981	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	4	43	theme	Yeast	761:765	arg1	β-glucan					767:774	Yeast β-glucan	761:774	Yeast β-glucan	761:774	Yeast β-glucan could regulate the diversity and composition of HFD-induced gut microbiota.					
34197112	0	44	from	Lactococcus	140:150	arg1	Microbiota					159:168	Gut Microbiota	155:168	Gut Microbiota	155:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	2	45	theme	gut	506:508	arg1	microbiota					510:519	gut microbiota	506:519	gut microbiota	506:519	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	5	46	theme	significant	927:937	arg1	correlation					948:958	significant positive correlation	927:958	significant positive correlation with metabolic changes	927:981	Moreover, the relative abundances of Lactobacillus and Lactococcus, having significant positive correlation with metabolic changes, were decreased by β-glucan, which might play a critical role in attenuation of MS. Our findings suggest that yeast β-glucan shows promising application as a prebiotic for preventing MS and regulating gut microbiota.					
34197112	0	47	attach	Linked	85:90	arg2	Improvement					0:10	Improvement	0:10	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan	0:80	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	0	47	attach	Linked	85:90	arg1	Proliferation					105:117	Inhibited Proliferation	95:117	Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota	95:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	2	48	theme	present	306:312	arg1	work					314:317	the present work	302:317	the present work	302:317	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	0	49	theme	Yeast	67:71	arg1	β-Glucan					73:80	Yeast β-Glucan	67:80	Yeast β-Glucan	67:80	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	0	50	from	Lactobacillus	122:134	arg1	Microbiota					159:168	Gut Microbiota	155:168	Gut Microbiota	155:168	Improvement of Metabolic Syndrome in High-Fat Diet-Induced Mice by Yeast β-Glucan Is Linked to Inhibited Proliferation of Lactobacillus and Lactococcus in Gut Microbiota.					
34197112	1	51	theme	syndrome	226:233	arg1	prevention					202:211	prevention	202:211	prevention of metabolic syndrome (MS) by dietary fibers	202:256	There is growing evidence that prevention of metabolic syndrome (MS) by dietary fibers is intricately linked to gut microbiota.					
34197112	2	52	from	relationship	485:496	arg1	MS					445:446	MS	445:446	MS	445:446	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34197112	2	52	from	relationship	485:496	arg1	microbiota					456:465	gut microbiota	452:465	gut microbiota	452:465	In the present work, the mice were fed a high-fat diet (HFD) and orally treated with yeast β-glucan to further examine the effects of β-glucan on MS and gut microbiota and the potential relationship between gut microbiota and its activity.					
34677958	2	0	theme	side	368:371	arg1	effects					373:379	various side effects	360:379	various side effects	360:379	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	8	1	theme	signaling	1228:1236	arg1	pathways					1238:1245	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	1194:1245	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers	1194:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	2	2	theme	various	360:366	arg1	effects					373:379	various side effects	360:379	various side effects	360:379	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	8	3	theme	inflammasome	1215:1226	arg1	pathways					1238:1245	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	1194:1245	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers	1194:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	3	4	from	effect	427:432	arg1	model					509:513	a colitis mouse model	493:513	a colitis mouse model	493:513	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	8	5	theme	gut	1292:1294	arg1	composition					1307:1317	gut microbiota composition	1292:1317	gut microbiota composition	1292:1317	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	4	6	theme	histopathological	738:754	arg1	score					756:760	histopathological score	738:760	histopathological score	738:760	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	4	7	theme	index	721:725	arg1	score					727:731	disease activity index score	704:731	disease activity index score	704:731	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	0	8	from	Colitis	68:74	arg1	Mice					79:82	Mice	79:82	Mice	79:82	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	7	9	theme	damaged	1067:1073	arg1	microbiota					1079:1088	the damaged gut microbiota	1063:1088	the damaged gut microbiota	1063:1088	B. cereus also rebalanced the damaged gut microbiota.					
34677958	4	10	theme	colon	679:683	arg1	shortening					692:701	colon length shortening	679:701	colon length shortening	679:701	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	4	11	theme	colitis	570:576	arg1	symptoms					578:585	The colitis symptoms	566:585	The colitis symptoms	566:585	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	0	12	theme	Barrier	122:128	arg1	Function					130:137	the Intestinal Barrier Function	107:137	the Intestinal Barrier Function	107:137	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	8	13	theme	pathways	1238:1245	arg1	regulation					1180:1189	the regulation	1176:1189	the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers	1176:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	2	14	theme	therapeutic	283:293	arg1	approaches					295:304	current therapeutic approaches	275:304	current therapeutic approaches for IBD	275:312	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	3	15	theme	new	439:441	arg1	HMPM18123					479:487	HMPM18123	479:487	HMPM18123	479:487	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	3	15	theme	new	439:441	arg1	strain					471:476	a new Bacillus cereus (B. cereus) strain	437:476	a new Bacillus cereus (B. cereus) strain (HMPM18123)	437:488	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	0	16	theme	Intestinal	111:120	arg1	Function					130:137	the Intestinal Barrier Function	107:137	the Intestinal Barrier Function	107:137	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	3	17	theme	positive	418:425	arg1	effect					427:432	the positive effect	414:432	the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model	414:513	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	2	18	theme	current	275:281	arg1	approaches					295:304	current therapeutic approaches	275:304	current therapeutic approaches for IBD	275:312	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	3	19	theme	Bacillus	443:450	arg1	HMPM18123					479:487	HMPM18123	479:487	HMPM18123	479:487	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	3	19	theme	Bacillus	443:450	arg1	strain					471:476	a new Bacillus cereus (B. cereus) strain	437:476	a new Bacillus cereus (B. cereus) strain (HMPM18123)	437:488	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	5	20	theme	intestinal	787:796	arg1	damage					817:822	intestinal epithelial barrier damage	787:822	intestinal epithelial barrier damage	787:822	The B. cereus mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression.					
34677958	3	21	theme	molecular	544:552	arg1	mechanisms					554:563	the underlying molecular mechanisms	529:563	the underlying molecular mechanisms	529:563	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	3	22	theme	mouse	503:507	arg1	model					509:513	a colitis mouse model	493:513	a colitis mouse model	493:513	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	4	23	theme	body	661:664	arg1	loss					673:676	decreased body weight loss	651:676	decreased body weight loss	651:676	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	5	24	theme	epithelial	798:807	arg1	damage					817:822	intestinal epithelial barrier damage	787:822	intestinal epithelial barrier damage	787:822	The B. cereus mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression.					
34677958	6	25	theme	signaling	1017:1025	arg1	pathways					1027:1034	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	983:1034	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	983:1034	Moreover, B. cereus exerted anti-inflammatory effects by regulating macrophage polarization and suppressing the TLR4-NF-κB-NLRP3 inflammasome signaling pathways.					
34677958	8	26	theme	molecular	1101:1109	arg1	mechanism					1111:1119	the molecular mechanism	1097:1119	the molecular mechanism of alleviating colitis by B. cereus treatment	1097:1165	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	5	27	theme	protein	855:861	arg1	expression					863:872	tight junction protein expression	840:872	tight junction protein expression	840:872	The B. cereus mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression.					
34677958	2	28	theme	limited	319:325	arg1	efficacy					327:334	limited efficacy	319:334	limited efficacy	319:334	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	4	29	theme	decreased	651:659	arg1	loss					673:676	decreased body weight loss	651:676	decreased body weight loss	651:676	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	5	30	theme	barrier	809:815	arg1	damage					817:822	intestinal epithelial barrier damage	787:822	intestinal epithelial barrier damage	787:822	The B. cereus mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression.					
34677958	6	31	theme	inflammasome	1004:1015	arg1	pathways					1027:1034	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	983:1034	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	983:1034	Moreover, B. cereus exerted anti-inflammatory effects by regulating macrophage polarization and suppressing the TLR4-NF-κB-NLRP3 inflammasome signaling pathways.					
34677958	4	32	dep	B.	610:611	arg1	cereus					613:618	cereus	613:618	cereus	613:618	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	1	33	theme	continuous	209:218	arg1	progress					220:227	continuous progress	209:227	continuous progress of inflammatory bowel disease (IBD)	209:263	Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD).					
34677958	4	34	theme	activity	712:719	arg1	score					727:731	disease activity index score	704:731	disease activity index score	704:731	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	6	35	theme	TLR4-NF-κB-NLRP3	987:1002	arg1	pathways					1027:1034	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	983:1034	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	983:1034	Moreover, B. cereus exerted anti-inflammatory effects by regulating macrophage polarization and suppressing the TLR4-NF-κB-NLRP3 inflammasome signaling pathways.					
34677958	0	36	theme	Function	130:137	arg1	Improvement					92:102	Improvement	92:102	Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation	92:187	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	3	37	theme	underlying	533:542	arg1	mechanisms					554:563	the underlying molecular mechanisms	529:563	the underlying molecular mechanisms	529:563	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	4	38	theme	B.	610:611	arg1	administration					620:633	the B. cereus administration	606:633	the B. cereus administration	606:633	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	0	39	theme	Sulfate	45:51	arg1	Colitis					68:74	Dextran Sulfate Sodium-Induced Colitis	37:74	Dextran Sulfate Sodium-Induced Colitis in Mice	37:82	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	8	40	dep	B.	1147:1148	arg1	cereus					1150:1155	cereus	1150:1155	cereus	1150:1155	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	8	41	theme	intestinal	1250:1259	arg1	barriers					1269:1276	intestinal mucosal barriers	1250:1276	intestinal mucosal barriers	1250:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	5	42	theme	tight	840:844	arg1	expression					863:872	tight junction protein expression	840:872	tight junction protein expression	840:872	The B. cereus mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression.					
34677958	0	43	theme	Dextran	37:43	arg1	Sulfate					45:51	Dextran Sulfate	37:51	Dextran Sulfate Sodium-Induced Colitis in Mice	37:82	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	0	44	theme	Anti-Inflammation	140:156	arg1	Improvement					92:102	Improvement	92:102	Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation	92:187	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	8	45	from	regulation	1180:1189	arg1	barriers					1269:1276	intestinal mucosal barriers	1250:1276	intestinal mucosal barriers	1250:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	1	46	theme	inflammatory	232:243	arg1	IBD					260:262	IBD	260:262	IBD	260:262	Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD).					
34677958	1	46	theme	inflammatory	232:243	arg1	disease					251:257	inflammatory bowel disease	232:257	inflammatory bowel disease (IBD)	232:263	Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD).					
34677958	8	47	theme	microbiota	1296:1305	arg1	composition					1307:1317	gut microbiota composition	1292:1317	gut microbiota composition	1292:1317	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	2	48	contain	have	314:317	arg1	approaches					295:304	current therapeutic approaches	275:304	current therapeutic approaches for IBD	275:312	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	2	48	contain	have	314:317	arg2	efficacy					327:334	limited efficacy	319:334	limited efficacy	319:334	However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects.					
34677958	0	49	theme	Gut	163:165	arg1	Modulation					178:187	Gut Microbiota Modulation	163:187	Gut Microbiota Modulation	163:187	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	7	50	theme	gut	1075:1077	arg1	microbiota					1079:1088	the damaged gut microbiota	1063:1088	the damaged gut microbiota	1063:1088	B. cereus also rebalanced the damaged gut microbiota.					
34677958	0	51	theme	Sodium-Induced	53:66	arg1	Colitis					68:74	Dextran Sulfate Sodium-Induced Colitis	37:74	Dextran Sulfate Sodium-Induced Colitis in Mice	37:82	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	8	52	theme	B.	1147:1148	arg1	treatment					1157:1165	B. cereus treatment	1147:1165	B. cereus treatment	1147:1165	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	8	53	from	pathways	1238:1245	arg1	barriers					1269:1276	intestinal mucosal barriers	1250:1276	intestinal mucosal barriers	1250:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	3	54	dep	Bacillus	443:450	arg1	cereus					452:457	cereus	452:457	cereus	452:457	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	3	54	dep	Bacillus	443:450	arg1	cereus					463:468	B. cereus	460:468	B. cereus	460:468	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	4	55	theme	length	685:690	arg1	shortening					692:701	colon length shortening	679:701	colon length shortening	679:701	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	6	56	theme	macrophage	943:952	arg1	polarization					954:965	macrophage polarization	943:965	macrophage polarization	943:965	Moreover, B. cereus exerted anti-inflammatory effects by regulating macrophage polarization and suppressing the TLR4-NF-κB-NLRP3 inflammasome signaling pathways.					
34677958	0	57	theme	Modulation	178:187	arg1	Improvement					92:102	Improvement	92:102	Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation	92:187	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	4	58	theme	weight	666:671	arg1	loss					673:676	decreased body weight loss	651:676	decreased body weight loss	651:676	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	4	59	theme	disease	704:710	arg1	score					727:731	disease activity index score	704:731	disease activity index score	704:731	The colitis symptoms were alleviated by the B. cereus administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score.					
34677958	1	60	theme	bowel	245:249	arg1	IBD					260:262	IBD	260:262	IBD	260:262	Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD).					
34677958	1	60	theme	bowel	245:249	arg1	disease					251:257	inflammatory bowel disease	232:257	inflammatory bowel disease (IBD)	232:263	Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD).					
34677958	0	61	theme	Microbiota	167:176	arg1	Modulation					178:187	Gut Microbiota Modulation	163:187	Gut Microbiota Modulation	163:187	Probiotic Bacillus cereus Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.					
34677958	3	62	theme	strain	471:476	arg1	effect					427:432	the positive effect	414:432	the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model	414:513	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	1	63	theme	disease	251:257	arg1	progress					220:227	continuous progress	209:227	continuous progress of inflammatory bowel disease (IBD)	209:263	Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD).					
34677958	5	64	theme	junction	846:853	arg1	expression					863:872	tight junction protein expression	840:872	tight junction protein expression	840:872	The B. cereus mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression.					
34677958	6	65	theme	anti-inflammatory	903:919	arg1	effects					921:927	anti-inflammatory effects	903:927	anti-inflammatory effects	903:927	Moreover, B. cereus exerted anti-inflammatory effects by regulating macrophage polarization and suppressing the TLR4-NF-κB-NLRP3 inflammasome signaling pathways.					
34677958	8	66	from	barriers	1269:1276	arg1	regulation					1180:1189	the regulation	1176:1189	the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers	1176:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	8	67	theme	mucosal	1261:1267	arg1	barriers					1269:1276	intestinal mucosal barriers	1250:1276	intestinal mucosal barriers	1250:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
34677958	3	68	theme	colitis	495:501	arg1	model					509:513	a colitis mouse model	493:513	a colitis mouse model	493:513	This study focused on exploring the positive effect of a new Bacillus cereus (B. cereus) strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms.					
34677958	8	69	theme	TLR4-NF-κB-NLRP3	1198:1213	arg1	pathways					1238:1245	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways	1194:1245	the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers	1194:1276	Thus, the molecular mechanism of alleviating colitis by B. cereus treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.					
32709357	4	0	theme	infrared	587:594	arg1	spectroscopy					596:607	Fourier-transform infrared spectroscopy	569:607	Fourier-transform infrared spectroscopy	569:607	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	1	1	theme	liquid	244:249	arg1	chromatography					251:264	hydrophilic interaction liquid chromatography	220:264	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	1	1	theme	liquid	244:249	arg1	HILIC					267:271	HILIC	267:271	HILIC	267:271	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	6	2	from	anions	978:983	arg1	effects					896:902	Different effects	886:902	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium	886:1021	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	2	3	attach	derived	342:348	arg2	phase					317:321	One obtained stationary phase	293:321	One obtained stationary phase (SilprAprImCl)	293:336	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	2	3	attach	derived	342:348	arg2	SilprAprImCl					324:335	SilprAprImCl	324:335	SilprAprImCl	324:335	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	2	3	attach	derived	342:348	arg1	reaction					362:369	direct reaction	355:369	direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	355:439	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	8	4	theme	phases	1289:1294	arg1	reliability					1257:1267	the reliability	1253:1267	the reliability of these stationary phases	1253:1294	Finally, the reliability of these stationary phases was demonstrated by the separation of oligosaccharides in real fructooligosaccharides samples.					
32709357	1	5	theme	chromatography	251:264	arg1	phases					285:290	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	5	6	from	behaviours	841:850	arg1	mode					880:883	HILIC mode	874:883	HILIC mode	874:883	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	4	7	theme	bonding	707:713	arg1	efficiency					715:724	satisfied bonding efficiency	697:724	satisfied bonding efficiency	697:724	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	6	8	from	temperature	936:946	arg1	effects					896:902	Different effects	886:902	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium	886:1021	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	7	9	theme	chromatographic	1177:1191	arg1	stability					1193:1201	chromatographic stability	1177:1201	chromatographic stability	1177:1201	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	4	10	theme	elemental	613:621	arg1	analysis					623:630	elemental analysis	613:630	elemental analysis	613:630	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	0	11	theme	interaction	102:112	arg1	chromatography					121:134	hydrophilic interaction liquid chromatography	90:134	hydrophilic interaction liquid chromatography	90:134	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	6	12	theme	imidazolium	949:959	arg1	unite					961:965	imidazolium unite	949:965	imidazolium unite	949:965	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	5	13	theme	materials	861:869	arg1	behaviours					841:850	the retention behaviours	827:850	the retention behaviours of these materials in HILIC mode	827:883	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	3	14	theme	ion	532:534	arg1	reaction					545:552	ion exchange reaction	532:552	ion exchange reaction	532:552	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	1	15	theme	novel	153:157	arg1	method					179:184	a novel imidazolium bonding method	151:184	a novel imidazolium bonding method	151:184	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	0	16	theme	hydrophilic	90:100	arg1	chromatography					121:134	hydrophilic interaction liquid chromatography	90:134	hydrophilic interaction liquid chromatography	90:134	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	7	17	theme	mobile	1134:1139	arg1	phases					1141:1146	mobile phases	1134:1146	mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections	1134:1241	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	3	18	theme	exchange	536:543	arg1	reaction					545:552	ion exchange reaction	532:552	ion exchange reaction	532:552	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	1	19	theme	imidazolium	159:169	arg1	method					179:184	a novel imidazolium bonding method	151:184	a novel imidazolium bonding method	151:184	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	0	20	theme	novel	2:6	arg1	bonding					20:26	A novel imidazolium bonding	0:26	A novel imidazolium bonding stationary phase	0:43	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	4	21	theme	satisfied	697:705	arg1	efficiency					715:724	satisfied bonding efficiency	697:724	satisfied bonding efficiency	697:724	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	1	22	theme	bonding	171:177	arg1	method					179:184	a novel imidazolium bonding method	151:184	a novel imidazolium bonding method	151:184	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	6	23	theme	paired	971:976	arg1	BF4-					991:994	BF4-	991:994	BF4-	991:994	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	23	theme	paired	971:976	arg1	Tf2N-					1001:1005	Tf2N-	1001:1005	Tf2N-	1001:1005	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	23	theme	paired	971:976	arg1	Cl-					986:988	Cl-	986:988	Cl-	986:988	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	23	theme	paired	971:976	arg1	anions					978:983	paired anions	971:983	paired anions (Cl-, BF4-, and Tf2N-)	971:1006	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	5	24	used	utilized	806:813	arg2	nucleosides					772:782	nucleosides	772:782	nucleosides	772:782	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	24	used	utilized	806:813	arg2	saccharides					759:769	saccharides	759:769	saccharides	759:769	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	24	used	utilized	806:813	arg2	nucleobases					789:799	nucleobases	789:799	nucleobases	789:799	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	24	used	utilized	806:813	arg2	compounds					741:749	Various polar compounds	727:749	Various polar compounds such as saccharides, nucleosides, and nucleobases	727:799	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	2	25	theme	stationary	306:315	arg1	SilprAprImCl					324:335	SilprAprImCl	324:335	SilprAprImCl	324:335	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	2	25	theme	stationary	306:315	arg1	phase					317:321	One obtained stationary phase	293:321	One obtained stationary phase (SilprAprImCl)	293:336	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	4	26	theme	efficiency	715:724	arg1	proofs					645:650	the proofs	641:650	the proofs of successful imidazolium immobilization and satisfied bonding efficiency	641:724	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	0	27	theme	bonding	20:26	arg1	phase					39:43	A novel imidazolium bonding stationary phase	0:43	A novel imidazolium bonding stationary phase	0:43	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	1	28	theme	stationary	274:283	arg1	phases					285:290	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	7	29	theme	ions	1103:1106	arg1	role					1079:1082	the role	1075:1082	the role of the imidazolium ions	1075:1106	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	7	29	theme	ions	1103:1106	arg1	mechanism					1061:1069	Separation mechanism	1050:1069	Separation mechanism	1050:1069	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	6	30	from	composition	916:926	arg1	effects					896:902	Different effects	886:902	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium	886:1021	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	2	31	theme	obtained	297:304	arg1	SilprAprImCl					324:335	SilprAprImCl	324:335	SilprAprImCl	324:335	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	2	31	theme	obtained	297:304	arg1	phase					317:321	One obtained stationary phase	293:321	One obtained stationary phase (SilprAprImCl)	293:336	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	2	32	theme	3-chloropropylated	411:428	arg1	gel					437:439	N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	379:439	N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	379:439	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	2	33	theme	silica	430:435	arg1	gel					437:439	N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	379:439	N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	379:439	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	1	34	theme	phases	285:290	arg1	synthesis					207:215	the synthesis	203:215	the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases	203:290	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	0	35	theme	liquid	114:119	arg1	chromatography					121:134	hydrophilic interaction liquid chromatography	90:134	hydrophilic interaction liquid chromatography	90:134	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	6	36	theme	column	929:934	arg1	temperature					936:946	column temperature	929:946	column temperature	929:946	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	8	37	from	separation	1320:1329	arg1	samples					1382:1388	real fructooligosaccharides samples	1354:1388	real fructooligosaccharides samples	1354:1388	Finally, the reliability of these stationary phases was demonstrated by the separation of oligosaccharides in real fructooligosaccharides samples.					
32709357	4	38	theme	successful	655:664	arg1	immobilization					678:691	successful imidazolium immobilization	655:691	successful imidazolium immobilization	655:691	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	5	39	theme	retention	831:839	arg1	behaviours					841:850	the retention behaviours	827:850	the retention behaviours of these materials in HILIC mode	827:883	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	4	40	theme	Fourier-transform	569:585	arg1	spectroscopy					596:607	Fourier-transform infrared spectroscopy	569:607	Fourier-transform infrared spectroscopy	569:607	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	7	41	theme	consecutive	1220:1230	arg1	injections					1232:1241	consecutive injections	1220:1241	consecutive injections	1220:1241	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	2	42	theme	-imidazole	396:405	arg1	gel					437:439	N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	379:439	N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	379:439	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	0	43	theme	stationary	28:37	arg1	phase					39:43	A novel imidazolium bonding stationary phase	0:43	A novel imidazolium bonding stationary phase	0:43	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	7	44	theme	imidazolium	1091:1101	arg1	ions					1103:1106	the imidazolium ions	1087:1106	the imidazolium ions	1087:1106	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	5	45	theme	HILIC	874:878	arg1	mode					880:883	HILIC mode	874:883	HILIC mode	874:883	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	7	46	theme	Separation	1050:1059	arg1	mechanism					1061:1069	Separation mechanism	1050:1069	Separation mechanism	1050:1069	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	4	47	theme	immobilization	678:691	arg1	proofs					645:650	the proofs	641:650	the proofs of successful imidazolium immobilization and satisfied bonding efficiency	641:724	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	5	48	theme	Various	727:733	arg1	compounds					741:749	Various polar compounds	727:749	Various polar compounds such as saccharides, nucleosides, and nucleobases	727:799	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	48	theme	Various	727:733	arg1	saccharides					759:769	saccharides	759:769	saccharides	759:769	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	48	theme	Various	727:733	arg1	nucleobases					789:799	nucleobases	789:799	nucleobases	789:799	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	48	theme	Various	727:733	arg1	nucleosides					772:782	nucleosides	772:782	nucleosides	772:782	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	6	49	theme	mobile	909:914	arg1	composition					916:926	mobile composition	909:926	mobile composition	909:926	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	7	50	dep	pH.	1163:1165	arg1	evaluated					1207:1215	evaluated	1207:1215	was evaluated by consecutive injections	1203:1241	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	6	51	from	effects	896:902	arg1	imidazolium					1011:1021	imidazolium	1011:1021	imidazolium	1011:1021	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	5	52	theme	polar	735:739	arg1	compounds					741:749	Various polar compounds	727:749	Various polar compounds such as saccharides, nucleosides, and nucleobases	727:799	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	52	theme	polar	735:739	arg1	saccharides					759:769	saccharides	759:769	saccharides	759:769	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	52	theme	polar	735:739	arg1	nucleobases					789:799	nucleobases	789:799	nucleobases	789:799	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	5	52	theme	polar	735:739	arg1	nucleosides					772:782	nucleosides	772:782	nucleosides	772:782	Various polar compounds such as saccharides, nucleosides, and nucleobases were utilized to evaluate the retention behaviours of these materials in HILIC mode.					
32709357	8	53	theme	oligosaccharides	1334:1349	arg1	separation					1320:1329	the separation	1316:1329	the separation of oligosaccharides in real fructooligosaccharides samples	1316:1388	Finally, the reliability of these stationary phases was demonstrated by the separation of oligosaccharides in real fructooligosaccharides samples.					
32709357	6	54	from	unite	961:965	arg1	effects					896:902	Different effects	886:902	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium	886:1021	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	55	dep	anions	978:983	arg1	BF4-					991:994	BF4-	991:994	BF4-	991:994	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	55	dep	anions	978:983	arg1	Tf2N-					1001:1005	Tf2N-	1001:1005	Tf2N-	1001:1005	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	55	dep	anions	978:983	arg1	Cl-					986:988	Cl-	986:988	Cl-	986:988	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	55	dep	anions	978:983	arg1	anions					978:983	paired anions	971:983	paired anions (Cl-, BF4-, and Tf2N-)	971:1006	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	6	56	theme	Different	886:894	arg1	effects					896:902	Different effects	886:902	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium	886:1021	Different effects from mobile composition, column temperature, imidazolium unite and paired anions (Cl-, BF4-, and Tf2N-) in imidazolium were proved and discussed.					
32709357	3	57	dep	materials	452:460	arg1	materials					452:460	Other two materials	442:460	Other two materials (SilprAprImBF4 and SilprAprImTf2N)	442:495	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	3	57	dep	materials	452:460	arg1	SilprAprImTf2N					481:494	SilprAprImTf2N	481:494	SilprAprImTf2N	481:494	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	3	57	dep	materials	452:460	arg1	SilprAprImBF4					463:475	SilprAprImBF4	463:475	SilprAprImBF4	463:475	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	8	58	theme	real	1354:1357	arg1	samples					1382:1388	real fructooligosaccharides samples	1354:1388	real fructooligosaccharides samples	1354:1388	Finally, the reliability of these stationary phases was demonstrated by the separation of oligosaccharides in real fructooligosaccharides samples.					
32709357	8	59	theme	fructooligosaccharides	1359:1380	arg1	samples					1382:1388	real fructooligosaccharides samples	1354:1388	real fructooligosaccharides samples	1354:1388	Finally, the reliability of these stationary phases was demonstrated by the separation of oligosaccharides in real fructooligosaccharides samples.					
32709357	4	60	theme	imidazolium	666:676	arg1	immobilization					678:691	successful imidazolium immobilization	655:691	successful imidazolium immobilization	655:691	Fourier-transform infrared spectroscopy and elemental analysis afforded the proofs of successful imidazolium immobilization and satisfied bonding efficiency.					
32709357	7	61	with	phases	1141:1146	arg1	pH.					1163:1165	pH.	1163:1165	different pH. Moreover, chromatographic stability was evaluated by consecutive injections	1153:1241	Separation mechanism and the role of the imidazolium ions were also investigated in mobile phases with different pH. Moreover, chromatographic stability was evaluated by consecutive injections.					
32709357	3	62	theme	Other	442:446	arg1	materials					452:460	Other two materials	442:460	Other two materials (SilprAprImBF4 and SilprAprImTf2N)	442:495	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	3	62	theme	Other	442:446	arg1	SilprAprImTf2N					481:494	SilprAprImTf2N	481:494	SilprAprImTf2N	481:494	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	3	62	theme	Other	442:446	arg1	SilprAprImBF4					463:475	SilprAprImBF4	463:475	SilprAprImBF4	463:475	Other two materials (SilprAprImBF4 and SilprAprImTf2N) were obtained from SilprAprImCl by ion exchange reaction, respectively.					
32709357	1	63	theme	hydrophilic	220:230	arg1	chromatography					251:264	hydrophilic interaction liquid chromatography	220:264	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	1	63	theme	hydrophilic	220:230	arg1	HILIC					267:271	HILIC	267:271	HILIC	267:271	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	8	64	theme	stationary	1278:1287	arg1	phases					1289:1294	these stationary phases	1272:1294	these stationary phases	1272:1294	Finally, the reliability of these stationary phases was demonstrated by the separation of oligosaccharides in real fructooligosaccharides samples.					
32709357	0	65	theme	imidazolium	8:18	arg1	bonding					20:26	A novel imidazolium bonding	0:26	A novel imidazolium bonding stationary phase	0:43	A novel imidazolium bonding stationary phase derived from N-(3-aminopropyl)-imidazole for hydrophilic interaction liquid chromatography.					
32709357	2	66	theme	direct	355:360	arg1	reaction					362:369	direct reaction	355:369	direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel	355:439	One obtained stationary phase (SilprAprImCl) was derived from direct reaction between N-(3-aminopropyl)-imidazole and 3-chloropropylated silica gel.					
32709357	1	67	theme	interaction	232:242	arg1	chromatography					251:264	hydrophilic interaction liquid chromatography	220:264	hydrophilic interaction liquid chromatography (HILIC) stationary phases	220:290	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32709357	1	67	theme	interaction	232:242	arg1	HILIC					267:271	HILIC	267:271	HILIC	267:271	In this work, a novel imidazolium bonding method was proposed for the synthesis of hydrophilic interaction liquid chromatography (HILIC) stationary phases.					
32757155	6	0	theme	release	890:896	arg1	profile					898:904	drug release profile	885:904	drug release profile	885:904	This involved looking into particle size, robustness, flowability, solid-state and drug release profile.					
32757155	13	1	theme	Liqui-Pellet	1746:1757	arg1	formulation					1759:1769	Liqui-Pellet formulation	1746:1769	Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets	1746:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	3	2	from	effect	498:503	arg1	Liqui-Pellet					585:596	Liqui-Pellet	585:596	Liqui-Pellet	585:596	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	9	3	from	effect	1180:1185	arg1	rate					1207:1210	the drug release rate	1190:1210	the drug release rate	1190:1210	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	4	4	theme	Liqui-Pellet	625:636	arg1	formulation					638:648	Liqui-Pellet formulation	625:648	Liqui-Pellet formulation in this study	625:662	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	11	5	theme	flow	1498:1501	arg1	property					1503:1510	exceptional flow property	1486:1510	exceptional flow property	1486:1510	The data shows Liqui-Pellet is resistant to friability, able to achieve exceptional flow property and have smooth surfaces, which is critical for applying coatings technology.					
32757155	1	6	theme	unique	188:193	arg1	advantages					195:204	unique advantages	188:204	unique advantages as well as displaying potential for commercial feasibility	188:263	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	10	7	theme	Liqui-Pellet	1339:1350	arg1	size					1352:1355	Liqui-Pellet size	1339:1355	Liqui-Pellet size	1339:1355	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	4	8	theme	METHODS	599:605	arg1	parameter					611:619	METHODS Key parameter	599:619	METHODS Key parameter for Liqui-Pellet formulation in this study	599:662	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	4	8	theme	METHODS	599:605	arg1	ratio					672:676	the ratio	668:676	the ratio of carrier and coating material	668:708	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	13	9	from	co-solvent	1816:1825	arg1	result					1776:1781	a result	1774:1781	a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets	1774:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	9	10	from	level	1161:1165	arg1	rate					1207:1210	the drug release rate	1190:1210	the drug release rate	1190:1210	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	12	11	theme	manufacturing	1639:1651	arg1	terms					1619:1623	terms	1619:1623	terms of commercial manufacturing	1619:1651	Such properties are ideal in terms of commercial manufacturing.					
32757155	9	12	theme	release	1199:1205	arg1	rate					1207:1210	the drug release rate	1190:1210	the drug release rate	1190:1210	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	3	13	theme	study	459:463	arg1	aim					447:449	the aim	443:449	the aim of this study	443:463	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	10	14	theme	release	1384:1390	arg1	rate					1392:1395	drug release rate	1379:1395	drug release rate	1379:1395	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	13	15	from	reduction	1685:1693	arg1	crystallinity					1710:1722	formulation crystallinity	1698:1722	formulation crystallinity	1698:1722	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	7	16	theme	Liqui-Pellet	925:936	arg1	morphology					911:920	The morphology	907:920	The morphology of Liqui-Pellet	907:936	The morphology of Liqui-Pellet was investigated by SEM.					
32757155	1	17	theme	AIM	78:80	arg1	Liqui-Pellet					82:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	1	17	theme	AIM	78:80	arg1	pill					143:146	an emerging next-generation oral pill	110:146	an emerging next-generation oral pill	110:146	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	12	18	theme	Such	1590:1593	arg1	properties					1595:1604	Such properties	1590:1604	Such properties	1590:1604	Such properties are ideal in terms of commercial manufacturing.					
32757155	13	19	theme	solubility	1786:1795	arg1	result					1776:1781	a result	1774:1781	a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets	1774:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	8	20	theme	major	1012:1016	arg1	effect					1018:1023	a major effect	1010:1023	a major effect	1010:1023	RESULTS It is found that R-value does not have a major effect on the success of Liqui-Pellet production.					
32757155	14	21	theme	drug	1934:1937	arg1	rate					1947:1950	Liqui-Pellet drug release rate	1921:1950	Liqui-Pellet drug release rate	1921:1950	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	4	22	theme	material	701:708	arg1	parameter					611:619	METHODS Key parameter	599:619	METHODS Key parameter for Liqui-Pellet formulation in this study	599:662	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	4	22	theme	material	701:708	arg1	ratio					672:676	the ratio	668:676	the ratio of carrier and coating material	668:708	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	5	23	theme	different	778:786	arg1	formulations					788:799	different formulations	778:799	different formulations	778:799	Tests were carried out to assess the physicochemical properties of different formulations.					
32757155	9	24	contain	have	1098:1101	arg2	effect					1106:1111	an effect	1103:1111	an effect on Liqui-Pellet size	1103:1132	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	9	24	contain	have	1098:1101	arg1	R-value					1077:1083	R-value	1077:1083	R-value	1077:1083	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	8	25	dep	RESULTS	963:969	arg1	found					977:981	found	977:981	is found that R-value does not have a major effect on the success of Liqui-Pellet production	974:1065	RESULTS It is found that R-value does not have a major effect on the success of Liqui-Pellet production.					
32757155	8	26	contain	have	1005:1008	arg2	effect					1018:1023	a major effect	1010:1023	a major effect	1010:1023	RESULTS It is found that R-value does not have a major effect on the success of Liqui-Pellet production.					
32757155	8	26	contain	have	1005:1008	arg1	R-value					988:994	R-value	988:994	R-value	988:994	RESULTS It is found that R-value does not have a major effect on the success of Liqui-Pellet production.					
32757155	1	27	theme	next-generation	122:136	arg1	Liqui-Pellet					82:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	1	27	theme	next-generation	122:136	arg1	pill					143:146	an emerging next-generation oral pill	110:146	an emerging next-generation oral pill	110:146	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	14	28	theme	production	1976:1985	arg1	rate					1995:1998	the production success rate	1972:1998	the production success rate	1972:1998	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	1	29	dep	advantages	195:204	arg1	displaying					217:226	displaying	217:226	as well as displaying potential for commercial feasibility	206:263	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	10	30	theme	Avicel	1227:1232	arg1	concentration					1240:1252	Avicel PH101 concentration	1227:1252	Avicel PH101 concentration	1227:1252	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	2	31	theme	release	418:424	arg1	rate					426:429	the drug release rate	409:429	the drug release rate	409:429	Since Liqui-Pellet technology is still in its infancy, it is important to explore the parameters that can affect its performance, particularly the drug release rate.					
32757155	8	32	theme	Liqui-Pellet	1043:1054	arg1	production					1056:1065	Liqui-Pellet production	1043:1065	Liqui-Pellet production	1043:1065	RESULTS It is found that R-value does not have a major effect on the success of Liqui-Pellet production.					
32757155	13	33	from	result	1776:1781	arg1	co-solvent					1816:1825	the co-solvent	1812:1825	the co-solvent used in the preparation of Liqui-Pellets	1812:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	0	34	theme	naproxen	55:62	arg1	Liqui-Pellet					64:75	naproxen Liqui-Pellet	55:75	naproxen Liqui-Pellet	55:75	Factors affecting performance and manufacturability of naproxen Liqui-Pellet.					
32757155	14	35	theme	Overall	1880:1886	arg1	CONCLUSION					1869:1878	CONCLUSION Overall	1869:1886	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.	1869:1999	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	9	36	theme	water	1147:1151	arg1	level					1161:1165	a certain water content level	1137:1165	a certain water content level	1137:1165	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	11	37	theme	smooth	1521:1526	arg1	surfaces					1528:1535	smooth surfaces	1521:1535	smooth surfaces	1521:1535	The data shows Liqui-Pellet is resistant to friability, able to achieve exceptional flow property and have smooth surfaces, which is critical for applying coatings technology.					
32757155	3	38	theme	Avicel	508:513	arg1	carrier					522:528	carrier	522:528	carrier	522:528	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	3	38	theme	Avicel	508:513	arg1	PH101					515:519	Avicel PH101	508:519	Avicel PH101 (carrier)	508:529	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	5	39	theme	physicochemical	748:762	arg1	properties					764:773	the physicochemical properties	744:773	the physicochemical properties of different formulations	744:799	Tests were carried out to assess the physicochemical properties of different formulations.					
32757155	13	40	from	solubility	1786:1795	arg1	co-solvent					1816:1825	the co-solvent	1812:1825	the co-solvent used in the preparation of Liqui-Pellets	1812:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	9	41	theme	Liqui-Pellet	1116:1127	arg1	size					1129:1132	Liqui-Pellet size	1116:1132	Liqui-Pellet size	1116:1132	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	6	42	theme	drug	885:888	arg1	profile					898:904	drug release profile	885:904	drug release profile	885:904	This involved looking into particle size, robustness, flowability, solid-state and drug release profile.					
32757155	13	43	theme	formulation	1698:1708	arg1	crystallinity					1710:1722	formulation crystallinity	1698:1722	formulation crystallinity	1698:1722	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	9	44	theme	content	1153:1159	arg1	level					1161:1165	a certain water content level	1137:1165	a certain water content level	1137:1165	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	9	45	theme	certain	1139:1145	arg1	level					1161:1165	a certain water content level	1137:1165	a certain water content level	1137:1165	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	4	46	from	formulation	638:648	arg1	study					658:662	this study	653:662	this study	653:662	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	13	47	theme	Liqui-Pellets	1854:1866	arg1	preparation					1839:1849	the preparation	1835:1849	the preparation of Liqui-Pellets	1835:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	9	48	from	effect	1106:1111	arg1	size					1129:1132	Liqui-Pellet size	1116:1132	Liqui-Pellet size	1116:1132	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	11	49	theme	exceptional	1486:1496	arg1	property					1503:1510	exceptional flow property	1486:1510	exceptional flow property	1486:1510	The data shows Liqui-Pellet is resistant to friability, able to achieve exceptional flow property and have smooth surfaces, which is critical for applying coatings technology.					
32757155	3	50	theme	Aerosil	535:541	arg1	ratio					566:570	Aerosil 300 (coating material) ratio	535:570	Aerosil 300 (coating material) ratio (R-value)	535:580	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	3	50	theme	Aerosil	535:541	arg1	R-value					573:579	R-value	573:579	R-value	573:579	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	9	51	theme	slight	1173:1178	arg1	effect					1180:1185	a slight effect	1171:1185	a slight effect on the drug release rate	1171:1210	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	3	52	theme	ratio	566:570	arg1	effect					498:503	the effect	494:503	the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet	494:596	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	4	53	theme	Key	607:609	arg1	parameter					611:619	METHODS Key parameter	599:619	METHODS Key parameter for Liqui-Pellet formulation in this study	599:662	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	4	53	theme	Key	607:609	arg1	ratio					672:676	the ratio	668:676	the ratio of carrier and coating material	668:708	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	14	54	dep	CONCLUSION	1869:1878	arg1	seems					1891:1895	seems	1891:1895	seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate	1891:1998	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	1	55	theme	oral	138:141	arg1	Liqui-Pellet					82:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	1	55	theme	oral	138:141	arg1	pill					143:146	an emerging next-generation oral pill	110:146	an emerging next-generation oral pill	110:146	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	10	56	from	decrease	1215:1222	arg1	concentration					1285:1297	Aerosil 300 concentration	1273:1297	Aerosil 300 concentration	1273:1297	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	10	56	from	decrease	1215:1222	arg1	formulations					1315:1326	Liqui-Pellet formulations	1302:1326	Liqui-Pellet formulations	1302:1326	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	10	56	from	decrease	1215:1222	arg1	concentration					1240:1252	Avicel PH101 concentration	1227:1252	Avicel PH101 concentration	1227:1252	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	9	57	theme	drug	1194:1197	arg1	rate					1207:1210	the drug release rate	1190:1210	the drug release rate	1190:1210	However, R-value does seem to have an effect on Liqui-Pellet size at a certain water content level and a slight effect on the drug release rate.					
32757155	12	58	theme	commercial	1628:1637	arg1	manufacturing					1639:1651	commercial manufacturing	1628:1651	commercial manufacturing	1628:1651	Such properties are ideal in terms of commercial manufacturing.					
32757155	12	59	from	terms	1619:1623	arg1	ideal					1610:1614	ideal	1610:1614	ideal	1610:1614	Such properties are ideal in terms of commercial manufacturing.					
32757155	4	60	theme	carrier	681:687	arg1	parameter					611:619	METHODS Key parameter	599:619	METHODS Key parameter for Liqui-Pellet formulation in this study	599:662	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	4	60	theme	carrier	681:687	arg1	ratio					672:676	the ratio	668:676	the ratio of carrier and coating material	668:708	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	2	61	theme	Liqui-Pellet	272:283	arg1	technology					285:294	Liqui-Pellet technology	272:294	Liqui-Pellet technology	272:294	Since Liqui-Pellet technology is still in its infancy, it is important to explore the parameters that can affect its performance, particularly the drug release rate.					
32757155	3	62	dep	Aerosil	535:541	arg1	coating					548:554	coating	548:554	coating material	548:563	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
32757155	12	63	from	ideal	1610:1614	arg1	terms					1619:1623	terms	1619:1623	terms of commercial manufacturing	1619:1651	Such properties are ideal in terms of commercial manufacturing.					
32757155	13	64	theme	drug	1804:1807	arg1	solubility					1786:1795	solubility	1786:1795	solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets	1786:1866	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	1	65	theme	promising	165:173	arg1	results					175:181	promising results	165:181	promising results	165:181	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	13	66	dep	show	1676:1679	arg1	both					1671:1674	both	1671:1674	both	1671:1674	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	10	67	theme	drug	1379:1382	arg1	rate					1392:1395	drug release rate	1379:1395	drug release rate	1379:1395	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	14	68	theme	release	1939:1945	arg1	rate					1947:1950	Liqui-Pellet drug release rate	1921:1950	Liqui-Pellet drug release rate	1921:1950	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	10	69	from	increase	1261:1268	arg1	concentration					1285:1297	Aerosil 300 concentration	1273:1297	Aerosil 300 concentration	1273:1297	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	10	69	from	increase	1261:1268	arg1	formulations					1315:1326	Liqui-Pellet formulations	1302:1326	Liqui-Pellet formulations	1302:1326	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	10	69	from	increase	1261:1268	arg1	concentration					1240:1252	Avicel PH101 concentration	1227:1252	Avicel PH101 concentration	1227:1252	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	13	70	dep	XRPD	1658:1661	arg1	The					1654:1656	The	1654:1656	The	1654:1656	The XRPD and DSC both show the reduction in formulation crystallinity, which is expected in Liqui-Pellet formulation as a result of solubility of the drug in the co-solvent used in the preparation of Liqui-Pellets.					
32757155	14	71	theme	Liqui-Pellet	1921:1932	arg1	rate					1947:1950	Liqui-Pellet drug release rate	1921:1950	Liqui-Pellet drug release rate	1921:1950	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	11	72	theme	able	1470:1473	arg1	friability					1458:1467	friability	1458:1467	friability	1458:1467	The data shows Liqui-Pellet is resistant to friability, able to achieve exceptional flow property and have smooth surfaces, which is critical for applying coatings technology.					
32757155	4	73	theme	coating	693:699	arg1	material					701:708	coating material	693:708	coating material	693:708	METHODS Key parameter for Liqui-Pellet formulation in this study was the ratio of carrier and coating material.					
32757155	5	74	theme	formulations	788:799	arg1	properties					764:773	the physicochemical properties	744:773	the physicochemical properties of different formulations	744:799	Tests were carried out to assess the physicochemical properties of different formulations.					
32757155	1	75	theme	emerging	113:120	arg1	Liqui-Pellet					82:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet	78:93	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	1	75	theme	emerging	113:120	arg1	pill					143:146	an emerging next-generation oral pill	110:146	an emerging next-generation oral pill	110:146	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	14	76	theme	success	1987:1993	arg1	rate					1995:1998	the production success rate	1972:1998	the production success rate	1972:1998	CONCLUSION Overall it seems that R-value can affect Liqui-Pellet drug release rate and size but not on the production success rate.					
32757155	10	77	theme	PH101	1234:1238	arg1	concentration					1240:1252	Avicel PH101 concentration	1227:1252	Avicel PH101 concentration	1227:1252	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	8	78	theme	production	1056:1065	arg1	success					1032:1038	the success	1028:1038	the success of Liqui-Pellet production	1028:1065	RESULTS It is found that R-value does not have a major effect on the success of Liqui-Pellet production.					
32757155	10	79	theme	Aerosil	1273:1279	arg1	concentration					1285:1297	Aerosil 300 concentration	1273:1297	Aerosil 300 concentration	1273:1297	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	2	80	theme	drug	413:416	arg1	rate					426:429	the drug release rate	409:429	the drug release rate	409:429	Since Liqui-Pellet technology is still in its infancy, it is important to explore the parameters that can affect its performance, particularly the drug release rate.					
32757155	0	81	theme	Liqui-Pellet	64:75	arg1	performance					18:28	performance	18:28	performance	18:28	Factors affecting performance and manufacturability of naproxen Liqui-Pellet.					
32757155	0	81	theme	Liqui-Pellet	64:75	arg1	manufacturability					34:50	manufacturability	34:50	manufacturability	34:50	Factors affecting performance and manufacturability of naproxen Liqui-Pellet.					
32757155	6	82	theme	particle	829:836	arg1	size					838:841	particle size	829:841	particle size	829:841	This involved looking into particle size, robustness, flowability, solid-state and drug release profile.					
32757155	10	83	theme	Liqui-Pellet	1302:1313	arg1	formulations					1315:1326	Liqui-Pellet formulations	1302:1326	Liqui-Pellet formulations	1302:1326	A decrease in Avicel PH101 concentration and an increase in Aerosil 300 concentration in Liqui-Pellet formulations can reduce Liqui-Pellet size and slightly increase drug release rate by 9% after 2 h.					
32757155	1	84	theme	commercial	242:251	arg1	feasibility					253:263	commercial feasibility	242:263	commercial feasibility	242:263	AIM Liqui-Pellet is potentially an emerging next-generation oral pill, which has shown promising results with unique advantages as well as displaying potential for commercial feasibility.					
32757155	11	85	theme	coatings	1569:1576	arg1	technology					1578:1587	coatings technology	1569:1587	coatings technology	1569:1587	The data shows Liqui-Pellet is resistant to friability, able to achieve exceptional flow property and have smooth surfaces, which is critical for applying coatings technology.					
32757155	3	86	theme	PH101	515:519	arg1	effect					498:503	the effect	494:503	the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet	494:596	Therefore, the aim of this study is to investigate thoroughly the effect of Avicel PH101 (carrier) and Aerosil 300 (coating material) ratio (R-value) in Liqui-Pellet.					
34666171	6	0	theme	microbiota	935:944	arg1	composition					946:956	altered colonic microbiota composition	919:956	altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	919:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	1	theme	short-chain	1095:1105	arg1	SCFAs					1120:1124	SCFAs	1120:1124	SCFAs	1120:1124	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	1	theme	short-chain	1095:1105	arg1	acids					1113:1117	short-chain fatty acids	1095:1117	bacterially derived short-chain fatty acids (SCFAs)	1075:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	4	2	theme	Citrobacter	571:581	arg1	colitis					619:625	Citrobacter rodentium-induced infectious murine colitis	571:625	Citrobacter rodentium-induced infectious murine colitis	571:625	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	6	3	from	presence	1063:1070	arg1	degradation					975:985	degradation	975:985	degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	975:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	4	theme	altered	919:925	arg1	composition					946:956	altered colonic microbiota composition	919:956	altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	919:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	4	5	theme	infectious	601:610	arg1	colitis					619:625	Citrobacter rodentium-induced infectious murine colitis	571:625	Citrobacter rodentium-induced infectious murine colitis	571:625	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	4	6	theme	gut	670:672	arg1	tissue					674:679	gut tissue	670:679	gut tissue	670:679	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	3	7	theme	synergistic	528:538	arg1	effect					540:545	the synergistic effect	524:545	the synergistic effect of CGN upon colitis	524:565	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	6	8	theme	dietary	886:892	arg1	consumption					900:910	high dietary λ-CGN consumption	881:910	high dietary λ-CGN consumption	881:910	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	7	9	from	mice	1264:1267	arg1	transplantation					1233:1247	fecal transplantation	1227:1247	fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice	1227:1306	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	6	10	theme	colonic	994:1000	arg1	layer					1008:1012	the colonic mucus layer	990:1012	the colonic mucus layer	990:1012	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	7	11	theme	fecal	1227:1231	arg1	transplantation					1233:1247	fecal transplantation	1227:1247	fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice	1227:1306	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	9	12	theme	present	1464:1470	arg1	study					1472:1476	The present study	1460:1476	The present study	1460:1476	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	8	13	theme	gut	1434:1436	arg1	metabolism					1448:1457	gut bacterial metabolism	1434:1457	gut bacterial metabolism	1434:1457	Our results confirm that λ-CGN may create an environment that favors inflammation by altering gut microbiota composition and gut bacterial metabolism.					
34666171	7	14	theme	altered	1152:1158	arg1	LPS					1166:1168	altered fecal LPS and SCFA levels	1152:1184	LPS	1166:1168	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	5	15	theme	λ-CGN	864:868	arg1	effect					854:859	the pro-inflammatory effect	833:859	the pro-inflammatory effect of λ-CGN	833:868	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	4	16	theme	λ-CGN	716:720	arg1	presence					704:711	the presence	700:711	the presence of λ-CGN	700:720	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	1	17	theme	half	156:159	arg1	century					163:169	half a century	156:169	nearly half a century	149:169	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	6	18	theme	level	1034:1038	arg1	degradation					975:985	degradation	975:985	degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	975:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	5	19	from	role	800:803	arg1	effect					854:859	the pro-inflammatory effect	833:859	the pro-inflammatory effect of λ-CGN	833:868	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	7	20	theme	fecal	1160:1164	arg1	LPS					1166:1168	altered fecal LPS and SCFA levels	1152:1184	LPS	1166:1168	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	9	21	theme	microbiota-barrier	1510:1527	arg1	target					1559:1564	an alternative target	1544:1564	an alternative target for ameliorating the colitis promoting effect of λ-CGN	1544:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	9	21	theme	microbiota-barrier	1510:1527	arg1	"					1533:1533	the "gut microbiota-barrier axis"	1501:1533	the "gut microbiota-barrier axis"	1501:1533	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	6	22	theme	fecal	1024:1028	arg1	level					1034:1038	a raised fecal LPS level	1015:1038	a raised fecal LPS level	1015:1038	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	0	23	theme	gut	96:98	arg1	microbiota					100:109	gut microbiota	96:109	gut microbiota	96:109	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	5	24	theme	transplantation	735:749	arg1	experiments					770:780	fecal transplantation and germ-free mice experiments	729:780	fecal transplantation and germ-free mice experiments	729:780	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	6	25	theme	layer	1008:1012	arg1	degradation					975:985	degradation	975:985	degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	975:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	7	26	theme	CGN-H-fed	1293:1301	arg1	mice					1303:1306	germ-free CGN-H-fed mice	1283:1306	germ-free CGN-H-fed mice	1283:1306	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	5	27	theme	germ-free	755:763	arg1	mice					765:768	germ-free mice	755:768	germ-free mice	755:768	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	9	28	theme	alternative	1547:1557	arg1	target					1559:1564	an alternative target	1544:1564	an alternative target for ameliorating the colitis promoting effect of λ-CGN	1544:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	9	28	theme	alternative	1547:1557	arg1	"					1533:1533	the "gut microbiota-barrier axis"	1501:1533	the "gut microbiota-barrier axis"	1501:1533	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	5	29	theme	mice	765:768	arg1	experiments					770:780	fecal transplantation and germ-free mice experiments	729:780	fecal transplantation and germ-free mice experiments	729:780	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	5	30	theme	microbiota	819:828	arg1	role					800:803	the role	796:803	the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN	796:868	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	0	31	theme	Citrobacter	26:36	arg1	colitis					67:73	Citrobacter rodentium-induced infectious colitis	26:73	Citrobacter rodentium-induced infectious colitis in mice	26:81	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	1	32	theme	ubiquitous	270:279	arg1	food					281:284	a ubiquitous food	268:284	a ubiquitous food additive	268:293	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	1	32	theme	ubiquitous	270:279	arg1	carrageenan					249:259	carrageenan	249:259	carrageenan (CGN)	249:265	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	6	33	theme	high	881:884	arg1	consumption					900:910	high dietary λ-CGN consumption	881:910	high dietary λ-CGN consumption	881:910	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	34	theme	decrease	1047:1054	arg1	degradation					975:985	degradation	975:985	degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	975:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	3	35	theme	gut	433:435	arg1	microbiota					437:446	gut microbiota	433:446	gut microbiota	433:446	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	2	36	theme	murine	396:401	arg1	colitis					403:409	murine colitis	396:409	murine colitis	396:409	Little is known about the mechanisms by which consumption of CGN aggravates the etiopathogenesis of murine colitis.					
34666171	0	37	theme	infectious	56:65	arg1	colitis					67:73	Citrobacter rodentium-induced infectious colitis	26:73	Citrobacter rodentium-induced infectious colitis in mice	26:81	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	1	38	theme	additive	286:293	arg1	food					281:284	a ubiquitous food	268:284	a ubiquitous food additive	268:293	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	1	38	theme	additive	286:293	arg1	carrageenan					249:259	carrageenan	249:259	carrageenan (CGN)	249:265	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	9	39	theme	promoting	1595:1603	arg1	effect					1605:1610	the colitis promoting effect	1583:1610	the colitis promoting effect of λ-CGN	1583:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	7	40	theme	layer	1134:1138	arg1	defects					1140:1146	Mucus layer defects	1128:1146	Mucus layer defects	1128:1146	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	6	41	from	degradation	975:985	arg1	presence					1063:1070	the presence	1059:1070	the presence of bacterially derived short-chain fatty acids (SCFAs)	1059:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	42	link	derived	1087:1093	arg1	SCFAs					1120:1124	SCFAs	1120:1124	SCFAs	1120:1124	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	42	link	derived	1087:1093	arg1	acids					1113:1117	short-chain fatty acids	1095:1117	bacterially derived short-chain fatty acids (SCFAs)	1075:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	8	43	theme	microbiota	1407:1416	arg1	composition					1418:1428	gut microbiota composition	1403:1428	gut microbiota composition	1403:1428	Our results confirm that λ-CGN may create an environment that favors inflammation by altering gut microbiota composition and gut bacterial metabolism.					
34666171	3	44	theme	barrier	463:469	arg1	integrity					471:479	intestinal barrier integrity	452:479	intestinal barrier integrity	452:479	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	7	45	from	mice	1303:1306	arg1	transplantation					1233:1247	fecal transplantation	1227:1247	fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice	1227:1306	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	4	46	theme	murine	612:617	arg1	colitis					619:625	Citrobacter rodentium-induced infectious murine colitis	571:625	Citrobacter rodentium-induced infectious murine colitis	571:625	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	3	47	theme	microbiota	437:446	arg1	analyses					421:428	analyses	421:428	analyses of gut microbiota and intestinal barrier integrity	421:479	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	6	48	theme	fatty	1107:1111	arg1	SCFAs					1120:1124	SCFAs	1120:1124	SCFAs	1120:1124	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	48	theme	fatty	1107:1111	arg1	acids					1113:1117	short-chain fatty acids	1095:1117	bacterially derived short-chain fatty acids (SCFAs)	1075:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	1	49	theme	carrageenan	249:259	arg1	profile					238:244	the safety profile	227:244	the safety profile of carrageenan (CGN), a ubiquitous food additive	227:293	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	6	50	theme	colonic	927:933	arg1	composition					946:956	altered colonic microbiota composition	919:956	altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs)	919:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	2	51	theme	colitis	403:409	arg1	etiopathogenesis					376:391	the etiopathogenesis	372:391	the etiopathogenesis of murine colitis	372:409	Little is known about the mechanisms by which consumption of CGN aggravates the etiopathogenesis of murine colitis.					
34666171	6	52	theme	derived	1087:1093	arg1	SCFAs					1120:1124	SCFAs	1120:1124	SCFAs	1120:1124	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	52	theme	derived	1087:1093	arg1	acids					1113:1117	short-chain fatty acids	1095:1117	bacterially derived short-chain fatty acids (SCFAs)	1075:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	4	53	theme	rodentium-induced	583:599	arg1	colitis					619:625	Citrobacter rodentium-induced infectious murine colitis	571:625	Citrobacter rodentium-induced infectious murine colitis	571:625	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	1	54	theme	scientific	176:185	arg1	community					187:195	the scientific community	172:195	the scientific community	172:195	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	1	54	theme	scientific	176:185	arg1	unable					206:211	unable	206:211	unable	206:211	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	6	55	theme	λ-CGN	894:898	arg1	consumption					900:910	high dietary λ-CGN consumption	881:910	high dietary λ-CGN consumption	881:910	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	6	56	theme	mucus	1002:1006	arg1	layer					1008:1012	the colonic mucus layer	990:1012	the colonic mucus layer	990:1012	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	4	57	theme	tissue	674:679	arg1	severity					658:665	the clinical severity	645:665	the clinical severity of gut tissue	645:679	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	4	57	theme	tissue	674:679	arg1	inflammation					628:639	inflammation	628:639	inflammation	628:639	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	6	58	from	decrease	1047:1054	arg1	presence					1063:1070	the presence	1059:1070	the presence of bacterially derived short-chain fatty acids (SCFAs)	1059:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	3	59	theme	CGN	550:552	arg1	effect					540:545	the synergistic effect	524:545	the synergistic effect of CGN upon colitis	524:565	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	5	60	theme	pro-inflammatory	837:852	arg1	effect					854:859	the pro-inflammatory effect	833:859	the pro-inflammatory effect of λ-CGN	833:868	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	8	61	theme	bacterial	1438:1446	arg1	metabolism					1448:1457	gut bacterial metabolism	1434:1457	gut bacterial metabolism	1434:1457	Our results confirm that λ-CGN may create an environment that favors inflammation by altering gut microbiota composition and gut bacterial metabolism.					
34666171	1	62	theme	a	161:161	arg1	century					163:169	half a century	156:169	nearly half a century	149:169	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	4	63	theme	clinical	649:656	arg1	severity					658:665	the clinical severity	645:665	the clinical severity of gut tissue	645:679	In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN.					
34666171	5	64	theme	fecal	729:733	arg1	transplantation					735:749	fecal transplantation	729:749	fecal transplantation	729:749	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	7	65	theme	germ-free	1209:1217	arg1	mice					1219:1222	germ-free mice	1209:1222	germ-free mice	1209:1222	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	9	66	theme	gut	1506:1508	arg1	target					1559:1564	an alternative target	1544:1564	an alternative target for ameliorating the colitis promoting effect of λ-CGN	1544:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	9	66	theme	gut	1506:1508	arg1	"					1533:1533	the "gut microbiota-barrier axis"	1501:1533	the "gut microbiota-barrier axis"	1501:1533	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	6	67	theme	LPS	1030:1032	arg1	level					1034:1038	a raised fecal LPS level	1015:1038	a raised fecal LPS level	1015:1038	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	9	68	theme	axis	1529:1532	arg1	target					1559:1564	an alternative target	1544:1564	an alternative target for ameliorating the colitis promoting effect of λ-CGN	1544:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	9	68	theme	axis	1529:1532	arg1	"					1533:1533	the "gut microbiota-barrier axis"	1501:1533	the "gut microbiota-barrier axis"	1501:1533	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	6	69	theme	raised	1017:1022	arg1	level					1034:1038	a raised fecal LPS level	1015:1038	a raised fecal LPS level	1015:1038	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	0	70	theme	intestinal	115:124	arg1	integrity					134:142	intestinal barrier integrity	115:142	intestinal barrier integrity	115:142	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	6	71	from	level	1034:1038	arg1	presence					1063:1070	the presence	1059:1070	the presence of bacterially derived short-chain fatty acids (SCFAs)	1059:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	7	72	theme	SCFA	1174:1177	arg1	levels					1179:1184	altered fecal LPS and SCFA levels	1152:1184	levels	1179:1184	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	6	73	with	Mice	871:874	arg1	consumption					900:910	high dietary λ-CGN consumption	881:910	high dietary λ-CGN consumption	881:910	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	0	74	from	colitis	67:73	arg1	mice					78:81	mice	78:81	mice	78:81	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	6	75	from	layer	1008:1012	arg1	presence					1063:1070	the presence	1059:1070	the presence of bacterially derived short-chain fatty acids (SCFAs)	1059:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
34666171	5	76	theme	intestinal	808:817	arg1	microbiota					819:828	intestinal microbiota	808:828	intestinal microbiota	808:828	Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN.					
34666171	2	77	theme	CGN	357:359	arg1	consumption					342:352	consumption	342:352	consumption of CGN	342:359	Little is known about the mechanisms by which consumption of CGN aggravates the etiopathogenesis of murine colitis.					
34666171	0	78	theme	rodentium-induced	38:54	arg1	colitis					67:73	Citrobacter rodentium-induced infectious colitis	26:73	Citrobacter rodentium-induced infectious colitis in mice	26:81	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	7	79	theme	germ-free	1283:1291	arg1	mice					1303:1306	germ-free CGN-H-fed mice	1283:1306	germ-free CGN-H-fed mice	1283:1306	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	3	80	theme	intestinal	452:461	arg1	integrity					471:479	intestinal barrier integrity	452:479	intestinal barrier integrity	452:479	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	9	81	theme	colitis	1587:1593	arg1	effect					1605:1610	the colitis promoting effect	1583:1610	the colitis promoting effect of λ-CGN	1583:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	0	82	theme	barrier	126:132	arg1	integrity					134:142	intestinal barrier integrity	115:142	intestinal barrier integrity	115:142	λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.					
34666171	7	83	theme	Mucus	1128:1132	arg1	defects					1140:1146	Mucus layer defects	1128:1146	Mucus layer defects	1128:1146	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	9	84	theme	λ-CGN	1615:1619	arg1	effect					1605:1610	the colitis promoting effect	1583:1610	the colitis promoting effect of λ-CGN	1583:1619	The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.					
34666171	7	85	theme	CGN-H-fed	1254:1262	arg1	mice					1264:1267	CGN-H-fed mice	1254:1267	CGN-H-fed mice	1254:1267	Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice.					
34666171	8	86	theme	gut	1403:1405	arg1	composition					1418:1428	gut microbiota composition	1403:1428	gut microbiota composition	1403:1428	Our results confirm that λ-CGN may create an environment that favors inflammation by altering gut microbiota composition and gut bacterial metabolism.					
34666171	3	87	theme	integrity	471:479	arg1	analyses					421:428	analyses	421:428	analyses of gut microbiota and intestinal barrier integrity	421:479	However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis.					
34666171	1	88	theme	safety	231:236	arg1	profile					238:244	the safety profile	227:244	the safety profile of carrageenan (CGN), a ubiquitous food additive	227:293	For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive.					
34666171	6	89	theme	acids	1113:1117	arg1	presence					1063:1070	the presence	1059:1070	the presence of bacterially derived short-chain fatty acids (SCFAs)	1059:1125	Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs).					
33217461	0	0	theme	amino	70:74	arg1	binding					81:87	amino acid binding	70:87	amino acid binding by periodate oxidation	70:110	Optimizing the properties of Zodo gum and examining its potential for amino acid binding by periodate oxidation.					
33217461	6	1	theme	content	1139:1145	arg1	NMR					1095:1097	1H-13C NMR	1088:1097	1H-13C NMR	1088:1097	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	6	1	theme	content	1139:1145	arg1	determination					1109:1121	determination	1109:1121	determination of the aldehyde content	1109:1145	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	6	1	theme	content	1139:1145	arg1	FTIR					1100:1103	FTIR	1100:1103	FTIR	1100:1103	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	6	2	theme	dialdehyde	1174:1183	arg1	groups					1185:1190	dialdehyde groups	1174:1190	dialdehyde groups	1174:1190	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	6	3	theme	groups	1185:1190	arg1	formation					1161:1169	the formation	1157:1169	the formation of dialdehyde groups	1157:1190	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	8	4	theme	DZG10	1559:1563	arg1	samples					1575:1581	DZG10 and DZG20 samples	1559:1581	DZG10 and DZG20 samples	1559:1581	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	1	5	theme	chemical	224:231	arg1	modification					233:244	chemical modification	224:244	chemical modification	224:244	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	7	6	theme	hydrophobic	1366:1376	arg1	properties					1378:1387	increased hydrophobic properties	1356:1387	increased hydrophobic properties	1356:1387	The AFM study of the DZG30 sample also showed over-oxidation and depolymerization, which could be associated with increased hydrophobic properties and the reduced viscosity.					
33217461	4	7	theme	polynomial	765:774	arg1	equations					776:784	Quadratic, linear polynomial equations	747:784	equations	776:784	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	1	8	theme	Zodo	132:135	arg1	gum					137:139	the Zodo gum	128:139	the Zodo gum exudated by Amygdalus scoparia spach	128:176	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	2	9	theme	volume	479:484	arg1	factors					451:457	4 factors	449:457	4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h)	449:549	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	10	theme	pH	525:526	arg1	factors					451:457	4 factors	449:457	4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h)	449:549	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	6	11	theme	aldehyde	1130:1137	arg1	content					1139:1145	the aldehyde content	1126:1145	the aldehyde content	1126:1145	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	8	12	theme	DZG20	1569:1573	arg1	samples					1575:1581	DZG10 and DZG20 samples	1559:1581	DZG10 and DZG20 samples	1559:1581	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	13	theme	amino	1534:1538	arg1	proteins					1549:1556	proteins	1549:1556	proteins	1549:1556	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	13	theme	amino	1534:1538	arg1	group					1540:1544	the amino group	1530:1544	the amino group of proteins	1530:1556	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	0	14	theme	acid	76:79	arg1	binding					81:87	amino acid binding	70:87	amino acid binding by periodate oxidation	70:110	Optimizing the properties of Zodo gum and examining its potential for amino acid binding by periodate oxidation.					
33217461	8	15	theme	DZG30	1429:1433	arg1	sample					1435:1440	the DZG30 sample	1425:1440	the DZG30 sample	1425:1440	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	4	16	theme	mentioned	890:898	arg1	variables					900:908	the mentioned variables	886:908	the mentioned variables	886:908	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	2	17	theme	sodium	462:467	arg1	6.4-19.2 mL					487:497	6.4-19.2 mL	487:497	6.4-19.2 mL	487:497	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	17	theme	sodium	462:467	arg1	volume					479:484	sodium periodate volume	462:484	sodium periodate volume (6.4-19.2 mL)	462:498	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	7	18	theme	increased	1356:1364	arg1	properties					1378:1387	increased hydrophobic properties	1356:1387	increased hydrophobic properties	1356:1387	The AFM study of the DZG30 sample also showed over-oxidation and depolymerization, which could be associated with increased hydrophobic properties and the reduced viscosity.					
33217461	4	19	theme	Quadratic	747:755	arg1	equations					776:784	Quadratic, linear polynomial equations	747:784	equations	776:784	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	8	20	contain	had	1442:1444	arg2	group					1490:1494	the dialdehyde factor group	1468:1494	the dialdehyde factor group	1468:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	20	contain	had	1442:1444	arg1	sample					1435:1440	the DZG30 sample	1425:1440	the DZG30 sample	1425:1440	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	20	contain	had	1442:1444	arg2	amount					1458:1463	the highest amount	1446:1463	the highest amount of the dialdehyde factor group	1446:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	2	21	theme	response	373:380	arg1	methodology					390:400	response surface methodology	373:400	response surface methodology (RSM)	373:406	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	21	theme	response	373:380	arg1	RSM					403:405	RSM	403:405	RSM	403:405	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	22	theme	time	538:541	arg1	factors					451:457	4 factors	449:457	4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h)	449:549	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	0	23	theme	periodate	92:100	arg1	oxidation					102:110	periodate oxidation	92:110	periodate oxidation	92:110	Optimizing the properties of Zodo gum and examining its potential for amino acid binding by periodate oxidation.					
33217461	2	24	theme	composite	420:428	arg1	CCD					438:440	CCD	438:440	CCD	438:440	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	24	theme	composite	420:428	arg1	design					430:435	central composite design	412:435	central composite design (CCD)	412:441	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	3	25	theme	test	606:609	arg1	variables					611:619	test variables	606:619	test variables	606:619	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	4	26	theme	linear	758:763	arg1	equations					776:784	Quadratic, linear polynomial equations	747:784	equations	776:784	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	8	27	theme	highest	1450:1456	arg1	amount					1458:1463	the highest amount	1446:1463	the highest amount of the dialdehyde factor group	1446:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	27	theme	highest	1450:1456	arg1	group					1490:1494	the dialdehyde factor group	1468:1494	the dialdehyde factor group	1468:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	6	28	theme	determination	1109:1121	arg1	results					1077:1083	The results	1073:1083	The results of 1H-13C NMR, FTIR and determination of the aldehyde content	1073:1145	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	2	29	theme	central	412:418	arg1	CCD					438:440	CCD	438:440	CCD	438:440	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	29	theme	central	412:418	arg1	design					430:435	central composite design	412:435	central composite design (CCD)	412:441	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	5	30	theme	samples	1064:1070	arg1	production					1027:1036	the production	1023:1036	the production of DZG10, DZG20 and DZG30 samples	1023:1070	Optimal test conditions, including pH = 3.9, T = 43 °C and Time = 3.5 h, were also determined for the production of DZG10, DZG20 and DZG30 samples.					
33217461	2	31	theme	oxidation	355:363	arg1	method					365:370	the periodate oxidation method	341:370	the periodate oxidation method	341:370	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	8	32	theme	industrial	1608:1617	arg1	applications					1619:1630	industrial applications	1608:1630	industrial applications due to the nonoccurrence of overoxidation	1608:1672	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	1	33	theme	dialdehyde	267:276	arg1	groups					278:283	dialdehyde groups	267:283	dialdehyde groups	267:283	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	6	34	theme	hemiacetal	1225:1234	arg1	form					1236:1239	the dominant hemiacetal form	1212:1239	the dominant hemiacetal form	1212:1239	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	6	35	with	equilibrium	1195:1205	arg1	form					1236:1239	the dominant hemiacetal form	1212:1239	the dominant hemiacetal form	1212:1239	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	0	36	theme	gum	34:36	arg1	properties					15:24	the properties	11:24	the properties of Zodo gum	11:36	Optimizing the properties of Zodo gum and examining its potential for amino acid binding by periodate oxidation.					
33217461	1	37	theme	groups	278:283	arg1	process					212:218	the periodate oxidation process	188:218	the periodate oxidation process for chemical modification	188:244	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	1	37	theme	groups	278:283	arg1	formation					254:262	the formation	250:262	the formation of dialdehyde groups	250:283	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	4	38	theme	high	842:845	arg1	R2					847:848	high R2	842:848	high R2	842:848	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	3	39	theme	Dialdehyde	552:561	arg1	gum					568:570	Dialdehyde Zodo gum	552:570	Dialdehyde Zodo gum (DZG)	552:576	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	3	39	theme	Dialdehyde	552:561	arg1	DZG					573:575	DZG	573:575	DZG	573:575	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	7	40	theme	DZG30	1263:1267	arg1	sample					1269:1274	the DZG30 sample	1259:1274	the DZG30 sample	1259:1274	The AFM study of the DZG30 sample also showed over-oxidation and depolymerization, which could be associated with increased hydrophobic properties and the reduced viscosity.					
33217461	1	41	theme	Amygdalus	153:161	arg1	spach					172:176	Amygdalus scoparia spach	153:176	Amygdalus scoparia spach	153:176	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	0	42	theme	Zodo	29:32	arg1	gum					34:36	Zodo gum	29:36	Zodo gum	29:36	Optimizing the properties of Zodo gum and examining its potential for amino acid binding by periodate oxidation.					
33217461	8	43	theme	dialdehyde	1472:1481	arg1	group					1490:1494	the dialdehyde factor group	1468:1494	the dialdehyde factor group	1468:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	4	44	theme	fit	834:836	arg1	Lack					826:829	the insignificant Lack	808:829	the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses	808:922	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	6	45	theme	dominant	1216:1223	arg1	form					1236:1239	the dominant hemiacetal form	1212:1239	the dominant hemiacetal form	1212:1239	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	1	46	dep	Amygdalus	153:161	arg1	scoparia					163:170	scoparia	163:170	scoparia	163:170	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	2	47	theme	surface	382:388	arg1	methodology					390:400	response surface methodology	373:400	response surface methodology (RSM)	373:406	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	47	theme	surface	382:388	arg1	RSM					403:405	RSM	403:405	RSM	403:405	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	4	48	theme	R2	847:848	arg1	Lack					826:829	the insignificant Lack	808:829	the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses	808:922	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	8	49	theme	due	1632:1634	arg1	applications					1619:1630	industrial applications	1608:1630	industrial applications due to the nonoccurrence of overoxidation	1608:1672	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	7	50	theme	sample	1269:1274	arg1	study					1250:1254	The AFM study	1242:1254	The AFM study of the DZG30 sample	1242:1274	The AFM study of the DZG30 sample also showed over-oxidation and depolymerization, which could be associated with increased hydrophobic properties and the reduced viscosity.					
33217461	5	51	theme	test	933:936	arg1	pH = 3.9					960:967	pH = 3.9	960:967	pH = 3.9	960:967	Optimal test conditions, including pH = 3.9, T = 43 °C and Time = 3.5 h, were also determined for the production of DZG10, DZG20 and DZG30 samples.					
33217461	5	51	theme	test	933:936	arg1	conditions					938:947	Optimal test conditions	925:947	Optimal test conditions	925:947	Optimal test conditions, including pH = 3.9, T = 43 °C and Time = 3.5 h, were also determined for the production of DZG10, DZG20 and DZG30 samples.					
33217461	3	52	theme	dialdehyde	660:669	arg1	content					671:677	dialdehyde content	660:677	dialdehyde content	660:677	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	3	53	dep	evaluating	708:717	arg1	addition					696:703	addition	696:703	addition	696:703	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	6	54	theme	FTIR	1100:1103	arg1	results					1077:1083	The results	1073:1083	The results of 1H-13C NMR, FTIR and determination of the aldehyde content	1073:1145	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	8	55	theme	overoxidation	1660:1672	arg1	nonoccurrence					1643:1655	the nonoccurrence	1639:1655	the nonoccurrence of overoxidation	1639:1672	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	2	56	theme	properties	315:324	arg1	Modification					286:297	Modification	286:297	Modification of the Zodo gum properties	286:324	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	8	57	theme	factor	1483:1488	arg1	group					1490:1494	the dialdehyde factor group	1468:1494	the dialdehyde factor group	1468:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	4	58	theme	insignificant	812:824	arg1	Lack					826:829	the insignificant Lack	808:829	the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses	808:922	Quadratic, linear polynomial equations were then fitted with the insignificant Lack of fit and high R2 to address the relationship between the mentioned variables and responses.					
33217461	7	59	theme	reduced	1397:1403	arg1	viscosity					1405:1413	the reduced viscosity	1393:1413	the reduced viscosity	1393:1413	The AFM study of the DZG30 sample also showed over-oxidation and depolymerization, which could be associated with increased hydrophobic properties and the reduced viscosity.					
33217461	7	60	theme	AFM	1246:1248	arg1	study					1250:1254	The AFM study	1242:1254	The AFM study of the DZG30 sample	1242:1274	The AFM study of the DZG30 sample also showed over-oxidation and depolymerization, which could be associated with increased hydrophobic properties and the reduced viscosity.					
33217461	5	61	theme	Optimal	925:931	arg1	pH = 3.9					960:967	pH = 3.9	960:967	pH = 3.9	960:967	Optimal test conditions, including pH = 3.9, T = 43 °C and Time = 3.5 h, were also determined for the production of DZG10, DZG20 and DZG30 samples.					
33217461	5	61	theme	Optimal	925:931	arg1	conditions					938:947	Optimal test conditions	925:947	Optimal test conditions	925:947	Optimal test conditions, including pH = 3.9, T = 43 °C and Time = 3.5 h, were also determined for the production of DZG10, DZG20 and DZG30 samples.					
33217461	2	62	theme	gum	311:313	arg1	properties					315:324	the Zodo gum properties	302:324	the Zodo gum properties	302:324	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	1	63	theme	periodate	192:200	arg1	process					212:218	the periodate oxidation process	188:218	the periodate oxidation process for chemical modification	188:244	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	2	64	theme	temperature	501:511	arg1	factors					451:457	4 factors	449:457	4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h)	449:549	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	3	65	theme	Zodo	563:566	arg1	gum					568:570	Dialdehyde Zodo gum	552:570	Dialdehyde Zodo gum (DZG)	552:576	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	3	65	theme	Zodo	563:566	arg1	DZG					573:575	DZG	573:575	DZG	573:575	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	6	66	theme	NMR	1095:1097	arg1	results					1077:1083	The results	1073:1083	The results of 1H-13C NMR, FTIR and determination of the aldehyde content	1073:1145	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	3	67	theme	rheological	723:733	arg1	parameters					735:744	the rheological parameters	719:744	the rheological parameters	719:744	Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheological parameters.					
33217461	2	68	theme	Zodo	306:309	arg1	properties					315:324	the Zodo gum properties	302:324	the Zodo gum properties	302:324	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	1	69	theme	oxidation	202:210	arg1	process					212:218	the periodate oxidation process	188:218	the periodate oxidation process for chemical modification	188:244	In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups.					
33217461	6	70	theme	1H-13C	1088:1093	arg1	NMR					1095:1097	1H-13C NMR	1088:1097	1H-13C NMR	1088:1097	The results of 1H-13C NMR, FTIR and determination of the aldehyde content indicated the formation of dialdehyde groups in equilibrium with the dominant hemiacetal form.					
33217461	2	71	theme	periodate	345:353	arg1	method					365:370	the periodate oxidation method	341:370	the periodate oxidation method	341:370	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	8	72	theme	proteins	1549:1556	arg1	proteins					1549:1556	proteins	1549:1556	proteins	1549:1556	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	72	theme	proteins	1549:1556	arg1	group					1540:1544	the amino group	1530:1544	the amino group of proteins	1530:1556	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	73	theme	group	1490:1494	arg1	amount					1458:1463	the highest amount	1446:1463	the highest amount of the dialdehyde factor group	1446:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	8	73	theme	group	1490:1494	arg1	group					1490:1494	the dialdehyde factor group	1468:1494	the dialdehyde factor group	1468:1494	Although the DZG30 sample had the highest amount of the dialdehyde factor group with the tendency to combine with the amino group of proteins, DZG10 and DZG20 samples could be recommended for industrial applications due to the nonoccurrence of overoxidation.					
33217461	2	74	theme	periodate	469:477	arg1	6.4-19.2 mL					487:497	6.4-19.2 mL	487:497	6.4-19.2 mL	487:497	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
33217461	2	74	theme	periodate	469:477	arg1	volume					479:484	sodium periodate volume	462:484	sodium periodate volume (6.4-19.2 mL)	462:498	Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 mL), temperature (35-55 °C), pH (3-5) and time (2-4 h).					
34231056	7	0	theme	significant	1464:1474	arg1	change					1476:1481	no significant change	1461:1481	no significant change	1461:1481	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	6	1	theme	crucial	1121:1127	arg1	effect					1129:1134	a crucial effect	1119:1134	a crucial effect	1119:1134	In addition, the composition of the mobile phase also had a crucial effect.					
34231056	2	2	theme	separation	401:410	arg1	ability					412:418	The separation ability	397:418	The separation ability of the CL-CD-MOF packed column	397:449	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	1	3	theme	diphenyl	306:313	arg1	carbonate					315:323	diphenyl carbonate	306:323	diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs)	306:394	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	8	4	theme	CL-CD-MOF	1620:1628	arg1	column					1630:1635	the CL-CD-MOF column	1616:1635	the CL-CD-MOF column	1616:1635	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	0	5	link	Cross-linked	0:11	arg1	framework-a					42:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	5	6	contain	had	1020:1022	arg1	column					1013:1018	CL-CD-MOF column	1003:1018	CL-CD-MOF column	1003:1018	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	5	6	contain	had	1020:1022	arg2	ability					1052:1058	a certain shape recognition ability	1024:1058	a certain shape recognition ability	1024:1058	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	4	7	theme	RP-HPLC	700:706	arg1	mode					708:711	RP-HPLC mode	700:711	RP-HPLC mode	700:711	It was found that baseline separation could be obtained in RP-HPLC mode and it was superior to commercial C18 column in separating xylene isomers.					
34231056	5	8	theme	dipole-dipole	844:856	arg1	interaction					858:868	dipole-dipole interaction	844:868	dipole-dipole interaction	844:868	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	8	9	from	process	1590:1596	arg1	column					1630:1635	the CL-CD-MOF column	1616:1635	the CL-CD-MOF column	1616:1635	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	5	10	theme	transfer	882:889	arg1	interaction					891:901	π-electron transfer interaction	871:901	π-electron transfer interaction	871:901	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	7	11	dep	ranges	1378:1383	arg1	%					1393:1393	0.17-1.1%	1385:1393	0.17-1.1%	1385:1393	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	7	11	dep	ranges	1378:1383	arg1	%					1404:1404	0.96-1.9%	1396:1404	0.96-1.9%	1396:1404	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	7	11	dep	ranges	1378:1383	arg1	%					1415:1415	0.23-1.7%	1407:1415	0.23-1.7%	1407:1415	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	7	11	dep	ranges	1378:1383	arg1	%					1430:1430	0.32-1.9%	1422:1430	0.32-1.9%	1422:1430	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	4	12	theme	C18	747:749	arg1	column					751:756	commercial C18 column	736:756	commercial C18 column	736:756	It was found that baseline separation could be obtained in RP-HPLC mode and it was superior to commercial C18 column in separating xylene isomers.					
34231056	8	13	from	separations	1601:1611	arg1	column					1630:1635	the CL-CD-MOF column	1616:1635	the CL-CD-MOF column	1616:1635	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	1	14	theme	cross-linked	155:166	arg1	CL-CD-MOF					208:216	CL-CD-MOF	208:216	CL-CD-MOF	208:216	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	14	theme	cross-linked	155:166	arg1	framework					197:205	The cross-linked γ-cyclodextrin metal-organic framework	151:205	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF)	151:217	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	7	15	theme	height	1270:1275	arg1	deviations					1235:1244	the relative standard deviations	1213:1244	the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes	1213:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	5	16	theme	der	937:939	arg1	force					947:951	van der Waals force	933:951	van der Waals force	933:951	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	9	17	theme	excellent	1716:1724	arg1	performance					1726:1736	The excellent performance	1712:1736	The excellent performance	1712:1736	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	7	18	dep	repeatability	1198:1210	arg1	ranges					1378:1383	the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%	1374:1430	the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%	1374:1430	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	2	19	dep	normal-phase	496:507	arg1	NP-					510:512	NP-	510:512	NP-	510:512	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	7	20	theme	use	1536:1538	arg1	years					1527:1531	at least 3 years	1516:1531	at least 3 years of use	1516:1538	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	6	21	contain	had	1115:1117	arg1	composition					1078:1088	the composition	1074:1088	the composition of the mobile phase	1074:1108	In addition, the composition of the mobile phase also had a crucial effect.					
34231056	6	21	contain	had	1115:1117	arg2	effect					1129:1134	a crucial effect	1119:1134	a crucial effect	1119:1134	In addition, the composition of the mobile phase also had a crucial effect.					
34231056	0	22	theme	series	106:111	arg1	separations					83:93	the separations	79:93	the separations of benzene series and polycyclic aromatic hydrocarbons	79:148	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	0	23	theme	polycyclic	117:126	arg1	hydrocarbons					137:148	polycyclic aromatic hydrocarbons	117:148	polycyclic aromatic hydrocarbons	117:148	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	7	24	theme	analytes	1353:1360	arg1	separations					1327:1337	six replicate separations	1313:1337	six replicate separations of the tested analytes	1313:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	9	25	theme	HPLC	1765:1768	arg1	material					1787:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	4	26	theme	xylene	772:777	arg1	isomers					779:785	xylene isomers	772:785	xylene isomers	772:785	It was found that baseline separation could be obtained in RP-HPLC mode and it was superior to commercial C18 column in separating xylene isomers.					
34231056	2	27	theme	packed	437:442	arg1	column					444:449	the CL-CD-MOF packed column	423:449	the CL-CD-MOF packed column	423:449	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	0	28	theme	Cross-linked	0:11	arg1	framework-a					42:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	1	29	theme	separate	328:335	arg1	series					345:350	separate benzene series	328:350	separate benzene series	328:350	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	6	30	theme	mobile	1097:1102	arg1	phase					1104:1108	the mobile phase	1093:1108	the mobile phase	1093:1108	In addition, the composition of the mobile phase also had a crucial effect.					
34231056	9	31	theme	phase	1781:1785	arg1	material					1787:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	2	32	theme	reverse-phase	472:484	arg1	modes					515:519	both reverse-phase (RP-) and normal-phase (NP-) modes	467:519	both reverse-phase (RP-) and normal-phase (NP-) modes	467:519	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	0	33	theme	metal-organic	28:40	arg1	framework-a					42:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	1	34	theme	γ-cyclodextrin	168:181	arg1	CL-CD-MOF					208:216	CL-CD-MOF	208:216	CL-CD-MOF	208:216	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	34	theme	γ-cyclodextrin	168:181	arg1	framework					197:205	The cross-linked γ-cyclodextrin metal-organic framework	151:205	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF)	151:217	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	7	35	theme	relative	1217:1224	arg1	deviations					1235:1244	the relative standard deviations	1213:1244	the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes	1213:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	1	36	theme	polycyclic	356:365	arg1	PAHs					390:393	PAHs	390:393	PAHs	390:393	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	36	theme	polycyclic	356:365	arg1	hydrocarbons					376:387	polycyclic aromatic hydrocarbons	356:387	polycyclic aromatic hydrocarbons (PAHs)	356:394	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	2	37	dep	reverse-phase	472:484	arg1	RP-					487:489	RP-	487:489	RP-	487:489	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	7	38	theme	satisfactory	1171:1182	arg1	stability					1184:1192	satisfactory stability	1171:1192	satisfactory stability	1171:1192	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	7	39	theme	width	1303:1307	arg1	deviations					1235:1244	the relative standard deviations	1213:1244	the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes	1213:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	5	40	theme	shape	1034:1038	arg1	ability					1052:1058	a certain shape recognition ability	1024:1058	a certain shape recognition ability	1024:1058	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	7	41	theme	half	1293:1296	arg1	width					1303:1307	half peak width	1293:1307	half peak width	1293:1307	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	9	42	theme	series	1840:1845	arg1	separation					1800:1809	separation	1800:1809	separation	1800:1809	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	9	42	theme	series	1840:1845	arg1	determination					1815:1827	determination	1815:1827	determination of benzene series and PAHs	1815:1854	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	7	43	theme	standard	1226:1233	arg1	deviations					1235:1244	the relative standard deviations	1213:1244	the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes	1213:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	7	44	dep	separations	1327:1337	arg1	replicate					1317:1325	replicate	1317:1325	replicate	1317:1325	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	9	45	theme	PAHs	1851:1854	arg1	separation					1800:1809	separation	1800:1809	separation	1800:1809	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	9	45	theme	PAHs	1851:1854	arg1	determination					1815:1827	determination	1815:1827	determination of benzene series and PAHs	1815:1854	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	7	46	theme	time	1259:1262	arg1	deviations					1235:1244	the relative standard deviations	1213:1244	the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes	1213:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	8	47	theme	enthalpy	1655:1662	arg1	change					1664:1669	negative enthalpy change	1646:1669	negative enthalpy change (ΔH)	1646:1674	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	8	47	theme	enthalpy	1655:1662	arg1	ΔH					1672:1673	ΔH	1672:1673	ΔH	1672:1673	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	7	48	theme	peak	1278:1281	arg1	area					1283:1286	peak area	1278:1286	peak area	1278:1286	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	8	49	theme	separations	1601:1611	arg1	process					1590:1596	the process	1586:1596	the process of separations on the CL-CD-MOF column	1586:1635	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	5	50	theme	hydrophobic	904:914	arg1	interaction					916:926	hydrophobic interaction	904:926	hydrophobic interaction	904:926	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	3	51	theme	molecular	608:616	arg1	simulations					618:628	molecular simulations	608:628	molecular simulations	608:628	The retention mechanisms of these compounds were discussed and confirmed by combining molecular simulations in detail.					
34231056	8	52	theme	entropy	1680:1686	arg1	ΔS					1696:1697	ΔS	1696:1697	ΔS	1696:1697	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	8	52	theme	entropy	1680:1686	arg1	change					1688:1693	entropy change	1680:1693	entropy change (ΔS)	1680:1698	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	5	53	theme	π-electron	871:880	arg1	interaction					891:901	π-electron transfer interaction	871:901	π-electron transfer interaction	871:901	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	1	54	theme	γ-cyclodextrin	251:264	arg1	γ-CD-MOF					291:298	γ-CD-MOF	291:298	γ-CD-MOF	291:298	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	54	theme	γ-cyclodextrin	251:264	arg1	framework					280:288	γ-cyclodextrin metal-organic framework	251:288	γ-cyclodextrin metal-organic framework (γ-CD-MOF)	251:299	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	4	55	theme	commercial	736:745	arg1	column					751:756	commercial C18 column	736:756	commercial C18 column	736:756	It was found that baseline separation could be obtained in RP-HPLC mode and it was superior to commercial C18 column in separating xylene isomers.					
34231056	7	56	theme	peak	1265:1268	arg1	height					1270:1275	peak height	1265:1275	peak height	1265:1275	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	5	57	theme	van	933:935	arg1	force					947:951	van der Waals force	933:951	van der Waals force	933:951	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	7	58	theme	retention	1249:1257	arg1	time					1259:1262	retention time	1249:1262	retention time	1249:1262	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	0	59	theme	benzene	98:104	arg1	series					106:111	benzene series	98:111	benzene series	98:111	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	5	60	theme	Waals	941:945	arg1	force					947:951	van der Waals force	933:951	van der Waals force	933:951	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	0	61	theme	hydrocarbons	137:148	arg1	separations					83:93	the separations	79:93	the separations of benzene series and polycyclic aromatic hydrocarbons	79:148	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	8	62	dep	controlled	1700:1709	arg1	change					1664:1669	negative enthalpy change	1646:1669	negative enthalpy change (ΔH)	1646:1674	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	8	62	dep	controlled	1700:1709	arg1	change					1688:1693	entropy change	1680:1693	entropy change (ΔS)	1680:1698	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	8	62	dep	controlled	1700:1709	arg1	ΔS					1696:1697	ΔS	1696:1697	ΔS	1696:1697	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	8	62	dep	controlled	1700:1709	arg1	ΔH					1672:1673	ΔH	1672:1673	ΔH	1672:1673	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	5	63	theme	CL-CD-MOF	1003:1011	arg1	column					1013:1018	CL-CD-MOF column	1003:1018	CL-CD-MOF column	1003:1018	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	0	64	theme	aromatic	128:135	arg1	hydrocarbons					137:148	polycyclic aromatic hydrocarbons	117:148	polycyclic aromatic hydrocarbons	117:148	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	7	65	theme	tested	1346:1351	arg1	analytes					1353:1360	the tested analytes	1342:1360	the tested analytes	1342:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	9	66	theme	promising	1755:1763	arg1	material					1787:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	8	67	from	column	1630:1635	arg1	process					1590:1596	the process	1586:1596	the process of separations on the CL-CD-MOF column	1586:1635	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	2	68	theme	column	444:449	arg1	ability					412:418	The separation ability	397:418	The separation ability of the CL-CD-MOF packed column	397:449	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	0	69	theme	γ-cyclodextrin	13:26	arg1	framework-a					42:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a	0:52	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	5	70	theme	chromatographic	971:985	arg1	separation					987:996	the chromatographic separation	967:996	the chromatographic separation	967:996	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	6	71	theme	phase	1104:1108	arg1	composition					1078:1088	the composition	1074:1088	the composition of the mobile phase	1074:1108	In addition, the composition of the mobile phase also had a crucial effect.					
34231056	9	72	theme	stationary	1770:1779	arg1	material					1787:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	CL-CD-MOF a promising HPLC stationary phase material	1743:1794	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	1	73	theme	benzene	337:343	arg1	series					345:350	separate benzene series	328:350	separate benzene series	328:350	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	0	74	theme	stationary	58:67	arg1	phase					69:73	stationary phase	58:73	stationary phase	58:73	Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.					
34231056	8	75	theme	Thermodynamic	1541:1553	arg1	characteristics					1555:1569	Thermodynamic characteristics	1541:1569	Thermodynamic characteristics	1541:1569	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	1	76	theme	aromatic	367:374	arg1	PAHs					390:393	PAHs	390:393	PAHs	390:393	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	76	theme	aromatic	367:374	arg1	hydrocarbons					376:387	polycyclic aromatic hydrocarbons	356:387	polycyclic aromatic hydrocarbons (PAHs)	356:394	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	7	77	theme	peak	1298:1301	arg1	width					1303:1307	half peak width	1293:1307	half peak width	1293:1307	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	2	78	theme	normal-phase	496:507	arg1	modes					515:519	both reverse-phase (RP-) and normal-phase (NP-) modes	467:519	both reverse-phase (RP-) and normal-phase (NP-) modes	467:519	The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes.					
34231056	1	79	link	cross-linked	155:166	arg1	CL-CD-MOF					208:216	CL-CD-MOF	208:216	CL-CD-MOF	208:216	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	79	link	cross-linked	155:166	arg1	framework					197:205	The cross-linked γ-cyclodextrin metal-organic framework	151:205	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF)	151:217	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	80	theme	metal-organic	183:195	arg1	CL-CD-MOF					208:216	CL-CD-MOF	208:216	CL-CD-MOF	208:216	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	80	theme	metal-organic	183:195	arg1	framework					197:205	The cross-linked γ-cyclodextrin metal-organic framework	151:205	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF)	151:217	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	3	81	theme	retention	526:534	arg1	mechanisms					536:545	The retention mechanisms	522:545	The retention mechanisms of these compounds	522:564	The retention mechanisms of these compounds were discussed and confirmed by combining molecular simulations in detail.					
34231056	7	82	theme	separation	1490:1499	arg1	efficiency					1501:1510	the separation efficiency	1486:1510	the separation efficiency for at least 3 years of use	1486:1538	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	5	83	theme	certain	1026:1032	arg1	ability					1052:1058	a certain shape recognition ability	1024:1058	a certain shape recognition ability	1024:1058	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	9	84	theme	benzene	1832:1838	arg1	series					1840:1845	benzene series	1832:1845	benzene series	1832:1845	The excellent performance made CL-CD-MOF a promising HPLC stationary phase material for separation and determination of benzene series and PAHs.					
34231056	8	85	theme	negative	1646:1653	arg1	change					1664:1669	negative enthalpy change	1646:1669	negative enthalpy change (ΔH)	1646:1674	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	8	85	theme	negative	1646:1653	arg1	ΔH					1672:1673	ΔH	1672:1673	ΔH	1672:1673	Thermodynamic characteristics indicated that the process of separations on the CL-CD-MOF column was both negative enthalpy change (ΔH) and entropy change (ΔS) controlled.					
34231056	1	86	theme	metal-organic	266:278	arg1	γ-CD-MOF					291:298	γ-CD-MOF	291:298	γ-CD-MOF	291:298	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	1	86	theme	metal-organic	266:278	arg1	framework					280:288	γ-cyclodextrin metal-organic framework	251:288	γ-cyclodextrin metal-organic framework (γ-CD-MOF)	251:299	The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs).					
34231056	5	87	theme	recognition	1040:1050	arg1	ability					1052:1058	a certain shape recognition ability	1024:1058	a certain shape recognition ability	1024:1058	The interaction between CL-CD-MOF and analytes, such as dipole-dipole interaction, π-electron transfer interaction, hydrophobic interaction, and van der Waals force, may dominate the chromatographic separation, and CL-CD-MOF column had a certain shape recognition ability.					
34231056	7	88	theme	area	1283:1286	arg1	deviations					1235:1244	the relative standard deviations	1213:1244	the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes	1213:1360	Moreover, the column demonstrated satisfactory stability and repeatability (the relative standard deviations of retention time, peak height, peak area, and half peak width for six replicate separations of the tested analytes were within the ranges 0.17-1.1%, 0.96-1.9%, 0.23-1.7%, and 0.32-1.9%, respectively) and there was no significant change in the separation efficiency for at least 3 years of use.					
34231056	4	89	theme	baseline	659:666	arg1	separation					668:677	baseline separation	659:677	baseline separation	659:677	It was found that baseline separation could be obtained in RP-HPLC mode and it was superior to commercial C18 column in separating xylene isomers.					
34231056	3	90	theme	compounds	556:564	arg1	mechanisms					536:545	The retention mechanisms	522:545	The retention mechanisms of these compounds	522:564	The retention mechanisms of these compounds were discussed and confirmed by combining molecular simulations in detail.					
32784986	3	0	theme	human	475:479	arg1	body					481:484	the human body	471:484	the human body	471:484	The results showed that all types of scaffolds should be safe for use in the human body.					
32784986	5	1	theme	scaffold	735:742	arg1	scaffold					735:742	the scaffold	731:742	the scaffold	731:742	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	1	theme	scaffold	735:742	arg1	amount					760:765	the optimal amount	748:765	the optimal amount of the particular components of the scaffold	748:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	1	theme	scaffold	735:742	arg1	composition					716:726	the optimal composition	704:726	the optimal composition of the scaffold	704:742	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	1	theme	scaffold	735:742	arg1	components					785:794	the particular components	770:794	the particular components of the scaffold	770:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	6	2	theme	PVA/HA/HAp	861:870	arg1	combination					872:882	the best PVA/HA/HAp combination	852:882	the best PVA/HA/HAp combination	852:882	Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.					
32784986	1	3	theme	-based	153:158	arg1	scaffold					160:167	polyvinyl alcohol (PVA) -based scaffold	129:167	polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp)	129:227	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	6	4	theme	best	856:859	arg1	combination					872:882	the best PVA/HA/HAp combination	852:882	the best PVA/HA/HAp combination	852:882	Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.					
32784986	1	5	theme	physical	235:242	arg1	crosslinking					244:255	physical crosslinking	235:255	physical crosslinking by freezing-thawing method	235:282	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	4	6	theme	better	569:574	arg1	adhesion					576:583	better adhesion	569:583	better adhesion of cells	569:592	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	5	7	theme	scaffold	803:810	arg1	components					785:794	the particular components	770:794	the particular components of the scaffold	770:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	4	8	theme	HAp	682:684	arg1	HAp					682:684	HAp	682:684	HAp	682:684	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	8	theme	HAp	682:684	arg1	amount					672:677	the amount	668:677	the amount of HAp	668:684	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	5	9	theme	optimal	708:714	arg1	composition					716:726	the optimal composition	704:726	the optimal composition of the scaffold	704:742	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	2	10	theme	hemolytic	382:390	arg1	test					392:395	hemolytic test	382:395	hemolytic test	382:395	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	4	11	dep	cells	526:530	arg1	MG-63					532:536	MG-63	532:536	MG-63	532:536	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	11	dep	cells	526:530	arg1	proliferation					548:560	their proliferation	542:560	their proliferation	542:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	11	dep	cells	526:530	arg1	cells					526:530	human osteoblast-like cells MG-63 and their proliferation	504:560	human osteoblast-like cells MG-63 and their proliferation	504:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	3	12	from	use	464:466	arg1	body					481:484	the human body	471:484	the human body	471:484	The results showed that all types of scaffolds should be safe for use in the human body.					
32784986	0	13	theme	Biological	0:9	arg1	Evaluation					11:20	Biological Evaluation	0:20	Biological Evaluation of Polyvinyl Alcohol	0:41	Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite.					
32784986	2	14	theme	biological	301:310	arg1	evaluation					312:321	biological evaluation	301:321	biological evaluation of scaffolds	301:334	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	1	15	theme	hyaluronic	183:192	arg1	HA					200:201	HA	200:201	HA	200:201	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	1	15	theme	hyaluronic	183:192	arg1	acid					194:197	hyaluronic acid	183:197	hyaluronic acid (HA)	183:202	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	0	16	theme	Polyvinyl	25:33	arg1	Alcohol					35:41	Polyvinyl Alcohol	25:41	Polyvinyl Alcohol	25:41	Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite.					
32784986	1	17	theme	freezing-thawing	260:275	arg1	method					277:282	freezing-thawing method	260:282	freezing-thawing method	260:282	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	5	18	theme	components	785:794	arg1	scaffold					735:742	the scaffold	731:742	the scaffold	731:742	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	18	theme	components	785:794	arg1	amount					760:765	the optimal amount	748:765	the optimal amount of the particular components of the scaffold	748:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	18	theme	components	785:794	arg1	composition					716:726	the optimal composition	704:726	the optimal composition of the scaffold	704:742	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	18	theme	components	785:794	arg1	components					785:794	the particular components	770:794	the particular components of the scaffold	770:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	4	19	theme	HA	617:618	arg1	presence					605:612	the presence	601:612	the presence of HA	601:618	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	20	theme	better	634:639	arg1	proliferation					641:653	better proliferation	634:653	better proliferation	634:653	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	3	21	theme	scaffolds	435:443	arg1	types					426:430	all types	422:430	all types of scaffolds	422:443	The results showed that all types of scaffolds should be safe for use in the human body.					
32784986	0	22	theme	Alcohol	35:41	arg1	Evaluation					11:20	Biological Evaluation	0:20	Biological Evaluation of Polyvinyl Alcohol	0:41	Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite.					
32784986	5	23	theme	particular	774:783	arg1	components					785:794	the particular components	770:794	the particular components of the scaffold	770:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	24	theme	optimal	752:758	arg1	scaffold					735:742	the scaffold	731:742	the scaffold	731:742	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	24	theme	optimal	752:758	arg1	amount					760:765	the optimal amount	748:765	the optimal amount of the particular components of the scaffold	748:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	5	24	theme	optimal	752:758	arg1	components					785:794	the particular components	770:794	the particular components of the scaffold	770:810	This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold.					
32784986	6	25	theme	ratio	894:898	arg1	3:1:2					900:904	the ratio 3:1:2	890:904	the ratio 3:1:2	890:904	Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.					
32784986	4	26	theme	cells	526:530	arg1	culturing					491:499	The culturing	487:499	The culturing of human osteoblast-like cells MG-63 and their proliferation	487:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	0	27	dep	Hydrogels	43:51	arg1	Enriched					53:60	Enriched	53:60	Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite	43:98	Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite.					
32784986	2	28	theme	swelling	337:344	arg1	degree					346:351	swelling degree	337:351	swelling degree	337:351	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	4	29	theme	osteoblast-like	510:524	arg1	MG-63					532:536	MG-63	532:536	MG-63	532:536	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	29	theme	osteoblast-like	510:524	arg1	proliferation					548:560	their proliferation	542:560	their proliferation	542:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	29	theme	osteoblast-like	510:524	arg1	cells					526:530	human osteoblast-like cells MG-63 and their proliferation	504:560	human osteoblast-like cells MG-63 and their proliferation	504:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	30	theme	human	504:508	arg1	MG-63					532:536	MG-63	532:536	MG-63	532:536	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	30	theme	human	504:508	arg1	proliferation					548:560	their proliferation	542:560	their proliferation	542:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	4	30	theme	human	504:508	arg1	cells					526:530	human osteoblast-like cells MG-63 and their proliferation	504:560	human osteoblast-like cells MG-63 and their proliferation	504:560	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	1	31	theme	polyvinyl	129:137	arg1	PVA					148:150	PVA	148:150	PVA	148:150	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	1	31	theme	polyvinyl	129:137	arg1	alcohol					139:145	polyvinyl alcohol	129:145	polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp)	129:227	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	4	32	theme	cells	588:592	arg1	adhesion					576:583	better adhesion	569:583	better adhesion of cells	569:592	The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp.					
32784986	1	33	theme	alcohol	139:145	arg1	scaffold					160:167	polyvinyl alcohol (PVA) -based scaffold	129:167	polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp)	129:227	This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method.					
32784986	2	34	theme	scaffolds	326:334	arg1	assessment					366:375	bioactivity assessment	354:375	bioactivity assessment	354:375	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	2	34	theme	scaffolds	326:334	arg1	test					392:395	hemolytic test	382:395	hemolytic test	382:395	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	2	34	theme	scaffolds	326:334	arg1	degree					346:351	swelling degree	337:351	swelling degree	337:351	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	2	34	theme	scaffolds	326:334	arg1	evaluation					312:321	biological evaluation	301:321	biological evaluation of scaffolds	301:334	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
32784986	0	35	theme	Hyaluronic	65:74	arg1	Acid					76:79	Hyaluronic Acid	65:79	Hyaluronic Acid	65:79	Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite.					
32784986	2	36	theme	bioactivity	354:364	arg1	assessment					366:375	bioactivity assessment	354:375	bioactivity assessment	354:375	We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test.					
34753907	5	0	theme	trimer	818:823	arg1	simulations					784:794	molecular dynamics simulations	765:794	molecular dynamics simulations of the HIV-1-envelope trimer	765:823	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	4	1	theme	antibody-antigen	532:547	arg1	complexes					549:557	antibody-antigen complexes	532:557	antibody-antigen complexes with extended AFADs (>3σ)	532:583	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	2	2	theme	Data	418:421	arg1	Bank					423:426	the Protein Data Bank	406:426	the Protein Data Bank	406:426	Here, we quantify AFAD for ~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank.					
34753907	6	3	theme	antibody	1056:1063	arg1	2909					1065:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909	1011:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	1011:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	0	4	theme	glycan-dense	122:133	arg1	surfaces					135:142	glycan-dense surfaces	122:142	glycan-dense surfaces	122:142	Extended antibody-framework-to-antigen distance observed exclusively with broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces.					
34753907	4	5	theme	extended	564:571	arg1	>3σ					580:582	>3σ	580:582	>3σ	580:582	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	4	5	theme	extended	564:571	arg1	AFADs					573:577	extended AFADs	564:577	extended AFADs (>3σ)	564:583	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	6	6	from	2909	1065:1068	arg1	complex					1073:1079	complex	1073:1079	complex with an N160K-envelope trimer	1073:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	2	7	from	complexes	393:401	arg1	Bank					423:426	the Protein Data Bank	406:426	the Protein Data Bank	406:426	Here, we quantify AFAD for ~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank.					
34753907	2	8	theme	Protein	410:416	arg1	Bank					423:426	the Protein Data Bank	406:426	the Protein Data Bank	406:426	Here, we quantify AFAD for ~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank.					
34753907	1	9	theme	antibody	298:305	arg1	recognition					307:317	antibody recognition	298:317	antibody recognition	298:317	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	5	10	theme	molecular	765:773	arg1	simulations					784:794	molecular dynamics simulations	765:794	molecular dynamics simulations of the HIV-1-envelope trimer	765:823	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	6	11	theme	hole-targeting	1022:1035	arg1	2909					1065:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909	1011:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	1011:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	5	12	theme	dynamics	775:782	arg1	simulations					784:794	molecular dynamics simulations	765:794	molecular dynamics simulations of the HIV-1-envelope trimer	765:823	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	6	13	from	complex	1073:1079	arg1	structure					998:1006	the cryo-EM structure	986:1006	the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	986:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	6	14	theme	glycan	1015:1020	arg1	2909					1065:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909	1011:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	1011:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	7	15	theme	glycan	1255:1260	arg1	hole					1262:1265	a glycan hole	1253:1265	a glycan hole enabling closer recognition	1253:1293	Overall, extended AFADs exclusively recognized densely glycosylated surfaces, with the introduction of a glycan hole enabling closer recognition.					
34753907	5	16	theme	High	671:674	arg1	R2 = 0.8110					689:699	R2 = 0.8110	689:699	R2 = 0.8110	689:699	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	5	16	theme	High	671:674	arg1	correlation					676:686	High correlation	671:686	High correlation (R2 = 0.8110)	671:700	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	1	17	theme	antibody	229:236	arg1	antigen					252:258	a protein antigen	242:258	a protein antigen -	242:260	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	1	17	theme	antibody	229:236	arg1	body					218:221	the body	214:221	the body of an antibody	214:236	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	3	18	theme	standard	490:497	arg1	σ					510:510	σ	510:510	σ	510:510	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	3	18	theme	standard	490:497	arg1	deviation					499:507	standard deviation	490:507	standard deviation (σ)	490:511	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	7	19	theme	hole	1262:1265	arg1	introduction					1237:1248	the introduction	1233:1248	the introduction of a glycan hole enabling closer recognition	1233:1293	Overall, extended AFADs exclusively recognized densely glycosylated surfaces, with the introduction of a glycan hole enabling closer recognition.					
34753907	7	20	theme	extended	1159:1166	arg1	AFADs					1168:1172	extended AFADs	1159:1172	extended AFADs	1159:1172	Overall, extended AFADs exclusively recognized densely glycosylated surfaces, with the introduction of a glycan hole enabling closer recognition.					
34753907	7	21	theme	glycosylated	1205:1216	arg1	surfaces					1218:1225	densely glycosylated surfaces	1197:1225	densely glycosylated surfaces	1197:1225	Overall, extended AFADs exclusively recognized densely glycosylated surfaces, with the introduction of a glycan hole enabling closer recognition.					
34753907	3	22	theme	gaussian	444:451	arg1	distribution					453:464	a gaussian distribution	442:464	a gaussian distribution	442:464	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	3	23	theme	deviation	499:507	arg1	mean					471:474	mean	471:474	mean of 16.3 Å and standard deviation (σ) of 2.4 Å	471:520	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	0	24	theme	antibody-framework-to-antigen	9:37	arg1	distance					39:46	Extended antibody-framework-to-antigen distance	0:46	Extended antibody-framework-to-antigen distance	0:46	Extended antibody-framework-to-antigen distance observed exclusively with broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces.					
34753907	6	25	theme	cryo-EM	990:996	arg1	structure					998:1006	the cryo-EM structure	986:1006	the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	986:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	2	26	theme	non-redundant	354:366	arg1	complexes					393:401	~2,000 non-redundant antibody-protein-antigen complexes	347:401	~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank	347:426	Here, we quantify AFAD for ~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank.					
34753907	6	27	gly	glycosylated	891:902	arg1	apex					911:914	the glycosylated trimer apex	887:914	the glycosylated trimer apex	887:914	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	3	28	theme	16.3 Å	479:484	arg1	mean					471:474	mean	471:474	mean of 16.3 Å and standard deviation (σ) of 2.4 Å	471:520	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	0	29	theme	Extended	0:7	arg1	distance					39:46	Extended antibody-framework-to-antigen distance	0:46	Extended antibody-framework-to-antigen distance	0:46	Extended antibody-framework-to-antigen distance observed exclusively with broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces.					
34753907	6	30	theme	long	837:840	arg1	AFADs					842:846	Especially long AFADs	826:846	Especially long AFADs	826:846	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	1	31	theme	Antibody-Framework-to-Antigen	145:173	arg1	AFAD					185:188	AFAD	185:188	AFAD	185:188	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	1	31	theme	Antibody-Framework-to-Antigen	145:173	arg1	Distance					175:182	Antibody-Framework-to-Antigen Distance	145:182	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.	145:318	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	6	32	from	structure	998:1006	arg1	complex					1073:1079	complex	1073:1079	complex with an N160K-envelope trimer	1073:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	7	33	gly	glycosylated	1205:1216	arg1	surfaces					1218:1225	densely glycosylated surfaces	1197:1225	densely glycosylated surfaces	1197:1225	Overall, extended AFADs exclusively recognized densely glycosylated surfaces, with the introduction of a glycan hole enabling closer recognition.					
34753907	4	34	theme	immunodeficiency	608:623	arg1	antibodies					659:668	human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies	602:668	human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies	602:668	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	1	35	theme	protein	244:250	arg1	antigen					252:258	a protein antigen	242:258	a protein antigen -	242:260	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	4	36	theme	human	602:606	arg1	HIV-1					639:643	HIV-1	639:643	HIV-1	639:643	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	4	36	theme	human	602:606	arg1	immunodeficiency					608:623	human immunodeficiency virus-type 1	602:636	human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies	602:668	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	6	37	theme	N160K-envelope	1089:1102	arg1	trimer					1104:1109	an N160K-envelope trimer	1086:1109	an N160K-envelope trimer	1086:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	4	38	theme	virus-type	625:634	arg1	HIV-1					639:643	HIV-1	639:643	HIV-1	639:643	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	4	38	theme	virus-type	625:634	arg1	immunodeficiency					608:623	human immunodeficiency virus-type 1	602:636	human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies	602:668	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	6	39	theme	HIV-1-neutralizing	1037:1054	arg1	2909					1065:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909	1011:1068	the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	1011:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	0	40	theme	HIV-1-neutralizing	80:97	arg1	antibodies					99:108	broad HIV-1-neutralizing antibodies	74:108	broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces	74:142	Extended antibody-framework-to-antigen distance observed exclusively with broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces.					
34753907	0	41	theme	broad	74:78	arg1	antibodies					99:108	broad HIV-1-neutralizing antibodies	74:108	broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces	74:142	Extended antibody-framework-to-antigen distance observed exclusively with broad HIV-1-neutralizing antibodies recognizing glycan-dense surfaces.					
34753907	6	42	theme	apex-glycan	960:970	arg1	N160K					978:982	N160K	978:982	N160K	978:982	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	6	42	theme	apex-glycan	960:970	arg1	hole					972:975	an apex-glycan hole	957:975	an apex-glycan hole (N160K)	957:983	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	6	43	with	complex	1073:1079	arg1	trimer					1104:1109	an N160K-envelope trimer	1086:1109	an N160K-envelope trimer	1086:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	7	44	theme	closer	1276:1281	arg1	recognition					1283:1293	closer recognition	1276:1293	closer recognition	1276:1293	Overall, extended AFADs exclusively recognized densely glycosylated surfaces, with the introduction of a glycan hole enabling closer recognition.					
34753907	5	45	theme	glycan	733:738	arg1	coverage					740:747	glycan coverage	733:747	glycan coverage	733:747	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	6	46	theme	trimer	904:909	arg1	apex					911:914	the glycosylated trimer apex	887:914	the glycosylated trimer apex	887:914	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	3	47	theme	2.4 Å	516:520	arg1	σ					510:510	σ	510:510	σ	510:510	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	3	47	theme	2.4 Å	516:520	arg1	16.3 Å					479:484	16.3 Å	479:484	16.3 Å	479:484	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	3	47	theme	2.4 Å	516:520	arg1	deviation					499:507	standard deviation	490:507	standard deviation (σ)	490:511	AFADs showed a gaussian distribution with mean of 16.3 Å and standard deviation (σ) of 2.4 Å.					
34753907	4	48	theme	-neutralizing	645:657	arg1	antibodies					659:668	human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies	602:668	human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies	602:668	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	6	49	theme	glycosylated	891:902	arg1	apex					911:914	the glycosylated trimer apex	887:914	the glycosylated trimer apex	887:914	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	2	50	theme	antibody-protein-antigen	368:391	arg1	complexes					393:401	~2,000 non-redundant antibody-protein-antigen complexes	347:401	~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank	347:426	Here, we quantify AFAD for ~2,000 non-redundant antibody-protein-antigen complexes in the Protein Data Bank.					
34753907	4	51	with	complexes	549:557	arg1	>3σ					580:582	>3σ	580:582	>3σ	580:582	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	4	51	with	complexes	549:557	arg1	AFADs					573:577	extended AFADs	564:577	extended AFADs (>3σ)	564:583	Notably, antibody-antigen complexes with extended AFADs (>3σ) were exclusively human immunodeficiency virus-type 1 (HIV-1)-neutralizing antibodies.					
34753907	1	52	dep	Distance	175:182	arg1	parameter					278:286	an important parameter	265:286	an important parameter governing antibody recognition	265:317	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	1	52	dep	Distance	175:182	arg1	distance					197:204	the distance	193:204	the distance between the body of an antibody and a protein antigen -	193:260	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	6	53	theme	shorter	1136:1142	arg1	AFAD					1144:1147	a substantially shorter AFAD	1120:1147	a substantially shorter AFAD	1120:1147	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
34753907	1	54	theme	important	268:276	arg1	parameter					278:286	an important parameter	265:286	an important parameter governing antibody recognition	265:317	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	1	54	theme	important	268:276	arg1	distance					197:204	the distance	193:204	the distance between the body of an antibody and a protein antigen -	193:260	Antibody-Framework-to-Antigen Distance (AFAD) - the distance between the body of an antibody and a protein antigen - is an important parameter governing antibody recognition.					
34753907	5	55	theme	HIV-1-envelope	803:816	arg1	trimer					818:823	the HIV-1-envelope trimer	799:823	the HIV-1-envelope trimer	799:823	High correlation (R2 = 0.8110) was observed between AFADs and glycan coverage, as assessed by molecular dynamics simulations of the HIV-1-envelope trimer.					
34753907	6	56	theme	2909	1065:1068	arg1	structure					998:1006	the cryo-EM structure	986:1006	the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer	986:1109	Especially long AFADs were observed for antibodies targeting the glycosylated trimer apex, and we tested the impact of introducing an apex-glycan hole (N160K); the cryo-EM structure of the glycan hole-targeting HIV-1-neutralizing antibody 2909 in complex with an N160K-envelope trimer revealed a substantially shorter AFAD.					
32283782	3	0	theme	factorial	591:599	arg1	plus/minus					577:586	plus/minus 1	577:588	plus/minus 1 (factorial points)	577:607	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	0	theme	factorial	591:599	arg1	points					601:606	factorial points	591:606	factorial points	591:606	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	6	1	theme	°C.	1130:1132	arg1	release					1139:1145	4 °C. Drug release	1128:1145	4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1128:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	4	2	theme	desirability	742:753	arg1	approach					755:762	the desirability approach	738:762	the desirability approach	738:762	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	9	3	theme	potential	1707:1715	arg1	drawbacks					1717:1725	the potential drawbacks	1703:1725	the potential drawbacks of systemic chemotherapy for tongue carcinoma	1703:1771	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	6	4	theme	better	1108:1113	arg1	stability					1115:1123	better stability	1108:1123	better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1108:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	3	5	theme	chitosan	486:493	arg1	concentrations					446:459	The concentrations	442:459	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point	442:628	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	8	6	theme	biological	1506:1515	arg1	activity					1517:1524	Enhanced biological activity	1497:1524	Enhanced biological activity of SIM	1497:1531	Enhanced biological activity of SIM was observed due to QRC enabling promising drug and polymer synergistic interaction.					
32283782	9	7	from	breakthrough	1657:1668	arg1	therapy					1683:1689	localized therapy	1673:1689	localized therapy	1673:1689	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	3	8	theme	particle	649:656	arg1	PS-Y1					664:668	PS-Y1	664:668	PS-Y1	664:668	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	8	theme	particle	649:656	arg1	size					658:661	particle size	649:661	particle size (PS-Y1)	649:669	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	6	9	theme	%	1102:1102	arg1	EE					1094:1095	the highest EE	1082:1095	the highest EE 79.67%	1082:1102	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	3	10	theme	different	507:515	arg1	plus/minus					577:586	plus/minus 1	577:588	plus/minus 1 (factorial points)	577:607	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	10	theme	different	507:515	arg1	levels					517:522	five different levels	502:522	five different levels	502:522	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	10	theme	different	507:515	arg1	alpha					536:540	plus/minus alpha	525:540	plus/minus alpha (+1.414 and -1.414: axial points)	525:574	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	10	theme	different	507:515	arg1	point					624:628	the center point	613:628	the center point	613:628	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	1	11	theme	optimized	191:199	arg1	NPs					250:252	NPs	250:252	NPs	250:252	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	11	theme	optimized	191:199	arg1	nanoparticles					235:247	optimized chitosan-coated Simvastatin (SIM) nanoparticles	191:247	optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD)	191:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	4	12	theme	poloxamer	790:798	arg1	188					800:802	poloxamer 188 0.24% and chitosan 0.43%	790:827	poloxamer 188 0.24% and chitosan 0.43%	790:827	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	9	13	theme	chemotherapy	1739:1750	arg1	drawbacks					1717:1725	the potential drawbacks	1703:1725	the potential drawbacks of systemic chemotherapy for tongue carcinoma	1703:1771	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	0	14	theme	Tongue	136:141	arg1	Carcinoma					143:151	Tongue Carcinoma	136:151	Tongue Carcinoma	136:151	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	5	15	theme	spherical	942:950	arg1	SIM-NPs					952:958	spherical SIM-NPs	942:958	spherical SIM-NPs	942:958	Scanning microscopy showed spherical SIM-NPs, indicating monodispersity in the range of 0.50 ± 0.04 nm with a charge of +32.42 mV.					
32283782	6	16	theme	controlled	1278:1287	arg1	manner					1289:1294	a more controlled manner	1271:1294	a more controlled manner	1271:1294	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	3	17	dep	+1.414	543:548	arg1	points					568:573	axial points	562:573	axial points	562:573	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	6	18	theme	79.67	1097:1101	arg1	%					1102:1102	%	1102:1102	%	1102:1102	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	9	19	theme	tongue	1756:1761	arg1	carcinoma					1763:1771	tongue carcinoma	1756:1771	tongue carcinoma	1756:1771	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	4	20	dep	%	808:808	arg1	%					827:827	0.43%	823:827	0.43%	823:827	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	7	21	theme	apoptosis	1390:1398	arg1	occurrence					1400:1409	apoptosis occurrence	1390:1409	apoptosis occurrence	1390:1409	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	6	22	theme	highest	1086:1092	arg1	EE					1094:1095	the highest EE	1082:1095	the highest EE 79.67%	1082:1102	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	6	23	theme	SIM-QRC	1152:1158	arg1	ISG					1170:1172	SIM-QRC NP-loaded ISG	1152:1172	SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1152:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	5	24	with	range	994:998	arg1	charge					1025:1030	a charge	1023:1030	a charge of +32.42 mV	1023:1043	Scanning microscopy showed spherical SIM-NPs, indicating monodispersity in the range of 0.50 ± 0.04 nm with a charge of +32.42 mV.					
32283782	8	25	theme	promising	1566:1574	arg1	drug					1576:1579	promising drug	1566:1579	promising drug	1566:1579	Enhanced biological activity of SIM was observed due to QRC enabling promising drug and polymer synergistic interaction.					
32283782	6	26	theme	optimized	1050:1058	arg1	formulation					1060:1070	The optimized formulation	1046:1070	The optimized formulation	1046:1070	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	3	27	theme	entrapment	672:681	arg1	EE-Y2					693:697	EE-Y2	693:697	EE-Y2	693:697	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	27	theme	entrapment	672:681	arg1	efficacy					683:690	entrapment efficacy	672:690	entrapment efficacy (EE-Y2)	672:698	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	6	28	theme	plain	1329:1333	arg1	SIM					1335:1337	plain SIM	1329:1337	plain SIM	1329:1337	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	1	29	theme	gel	276:278	arg1	formulation					286:296	an in situ gel (ISG) formulation	265:296	an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD)	265:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	3	30	theme	stability	704:712	arg1	SI-Y3					721:725	SI-Y3	721:725	SI-Y3	721:725	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	30	theme	stability	704:712	arg1	index					714:718	stability index	704:718	stability index (SI-Y3)	704:726	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	1	31	theme	central	318:324	arg1	FCCCD					344:348	FCCCD	344:348	FCCCD	344:348	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	31	theme	central	318:324	arg1	design					336:341	a face-centered central composite design	302:341	a face-centered central composite design (FCCCD)	302:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	7	32	from	increase	1378:1385	arg1	occurrence					1400:1409	apoptosis occurrence	1390:1409	apoptosis occurrence	1390:1409	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	8	33	theme	synergistic	1593:1603	arg1	interaction					1605:1615	polymer synergistic interaction	1585:1615	polymer synergistic interaction	1585:1615	Enhanced biological activity of SIM was observed due to QRC enabling promising drug and polymer synergistic interaction.					
32283782	1	34	theme	study	171:175	arg1	goal					158:161	The goal	154:161	The goal of this study	154:175	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	0	35	theme	Effective	90:98	arg1	Activity					119:126	Effective Anti-Proliferative Activity	90:126	Effective Anti-Proliferative Activity against Tongue Carcinoma	90:151	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	7	36	theme	suppressor	1470:1479	arg1	levels					1489:1494	the tumor suppressor protein levels	1460:1494	the tumor suppressor protein levels	1460:1494	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	4	37	theme	0.43	823:826	arg1	%					827:827	0.43%	823:827	0.43%	823:827	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	6	38	dep	96	1205:1206	arg1	to					1202:1203	to	1202:1203	to	1202:1203	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	4	39	theme	SIM-QRC	892:898	arg1	ISG					910:912	SIM-QRC NP-loaded ISG	892:912	SIM-QRC NP-loaded ISG	892:912	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	2	40	theme	Coated	352:357	arg1	SIM-NPs					359:365	Coated SIM-NPs	352:365	Coated SIM-NPs	352:365	Coated SIM-NPs were doped with Quercetin (QRC) using a modified nanoprecipitation method.					
32283782	0	41	theme	In	0:1	arg1	Gel					8:10	In Situ Gel	0:10	In Situ Gel	0:10	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	1	42	theme	in	268:269	arg1	formulation					286:296	an in situ gel (ISG) formulation	265:296	an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD)	265:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	5	43	theme	mV	1042:1043	arg1	charge					1025:1030	a charge	1023:1030	a charge of +32.42 mV	1023:1043	Scanning microscopy showed spherical SIM-NPs, indicating monodispersity in the range of 0.50 ± 0.04 nm with a charge of +32.42 mV.					
32283782	4	44	contain	containing	779:788	arg1	formulation					767:777	a formulation	765:777	a formulation containing poloxamer 188 0.24% and chitosan 0.43%	765:827	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	4	44	contain	containing	779:788	arg2	188					800:802	poloxamer 188 0.24% and chitosan 0.43%	790:827	poloxamer 188 0.24% and chitosan 0.43%	790:827	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	4	45	theme	optimum	858:864	arg1	formulation					866:876	optimum formulation	858:876	optimum formulation	858:876	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	1	46	theme	ISG	281:283	arg1	formulation					286:296	an in situ gel (ISG) formulation	265:296	an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD)	265:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	2	47	theme	nanoprecipitation	416:432	arg1	method					434:439	a modified nanoprecipitation method	405:439	a modified nanoprecipitation method	405:439	Coated SIM-NPs were doped with Quercetin (QRC) using a modified nanoprecipitation method.					
32283782	5	48	theme	nm	1015:1016	arg1	range					994:998	the range	990:998	the range of 0.50 ± 0.04 nm with a charge of +32.42 mV	990:1043	Scanning microscopy showed spherical SIM-NPs, indicating monodispersity in the range of 0.50 ± 0.04 nm with a charge of +32.42 mV.					
32283782	3	49	theme	axial	562:566	arg1	points					568:573	axial points	562:573	axial points	562:573	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	0	50	theme	Simvastatin	40:50	arg1	Nanoparticles					52:64	Chitosan-Coated Simvastatin Nanoparticles	24:64	Chitosan-Coated Simvastatin Nanoparticles	24:64	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	0	51	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	9	52	theme	localized	1673:1681	arg1	therapy					1683:1689	localized therapy	1673:1689	localized therapy	1673:1689	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	6	53	theme	Drug	1134:1137	arg1	release					1139:1145	4 °C. Drug release	1128:1145	4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1128:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	6	54	theme	4	1128:1128	arg1	°C.					1130:1132	°C.	1130:1132	°C.	1130:1132	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	6	55	theme	SIM-QRC	1313:1319	arg1	NPs					1321:1323	SIM-QRC NPs	1313:1323	SIM-QRC NPs	1313:1323	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	2	56	theme	modified	407:414	arg1	method					434:439	a modified nanoprecipitation method	405:439	a modified nanoprecipitation method	405:439	Coated SIM-NPs were doped with Quercetin (QRC) using a modified nanoprecipitation method.					
32283782	3	57	theme	center	617:622	arg1	levels					517:522	five different levels	502:522	five different levels	502:522	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	57	theme	center	617:622	arg1	point					624:628	the center point	613:628	the center point	613:628	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	8	58	theme	Enhanced	1497:1504	arg1	activity					1517:1524	Enhanced biological activity	1497:1524	Enhanced biological activity of SIM	1497:1531	Enhanced biological activity of SIM was observed due to QRC enabling promising drug and polymer synergistic interaction.					
32283782	7	59	theme	caspase-3	1419:1427	arg1	mediation					1429:1437	caspase-3 mediation	1419:1437	caspase-3 mediation	1419:1437	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	9	60	theme	systemic	1730:1737	arg1	chemotherapy					1739:1750	systemic chemotherapy	1730:1750	systemic chemotherapy	1730:1750	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	1	61	theme	chitosan-coated	201:215	arg1	Simvastatin					217:227	chitosan-coated Simvastatin	201:227	optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD)	191:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	61	theme	chitosan-coated	201:215	arg1	SIM					230:232	SIM	230:232	SIM	230:232	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	3	62	theme	plus/minus	525:534	arg1	+1.414					543:548	+1.414	543:548	+1.414	543:548	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	62	theme	plus/minus	525:534	arg1	levels					517:522	five different levels	502:522	five different levels	502:522	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	62	theme	plus/minus	525:534	arg1	alpha					536:540	plus/minus alpha	525:540	plus/minus alpha (+1.414 and -1.414: axial points)	525:574	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	3	62	theme	plus/minus	525:534	arg1	-1.414					554:559	-1.414	554:559	-1.414	554:559	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	8	63	theme	SIM	1529:1531	arg1	activity					1517:1524	Enhanced biological activity	1497:1524	Enhanced biological activity of SIM	1497:1531	Enhanced biological activity of SIM was observed due to QRC enabling promising drug and polymer synergistic interaction.					
32283782	6	64	dep	ISG	1170:1172	arg1	slower					1178:1183	slower	1178:1183	slower	1178:1183	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	6	65	theme	NP-loaded	1160:1168	arg1	ISG					1170:1172	SIM-QRC NP-loaded ISG	1152:1172	SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1152:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	7	66	theme	considerable	1365:1376	arg1	increase					1378:1385	a considerable increase	1363:1385	a considerable increase in apoptosis occurrence	1363:1409	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	5	67	theme	Scanning	915:922	arg1	microscopy					924:933	Scanning microscopy	915:933	Scanning microscopy	915:933	Scanning microscopy showed spherical SIM-NPs, indicating monodispersity in the range of 0.50 ± 0.04 nm with a charge of +32.42 mV.					
32283782	0	68	theme	Promising	67:75	arg1	Delivery					77:84	Promising Delivery	67:84	Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma	67:151	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	7	69	theme	ISG	1340:1342	arg1	formulation					1344:1354	ISG formulation	1340:1354	ISG formulation	1340:1354	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	4	70	dep	188	800:802	arg1	chitosan					814:821	chitosan	814:821	chitosan	814:821	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	4	70	dep	188	800:802	arg1	%					808:808	0.24%	804:808	0.24%	804:808	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	1	71	theme	face-centered	304:316	arg1	FCCCD					344:348	FCCCD	344:348	FCCCD	344:348	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	71	theme	face-centered	304:316	arg1	design					336:341	a face-centered central composite design	302:341	a face-centered central composite design (FCCCD)	302:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	72	dep	in	268:269	arg1	situ					271:274	situ	271:274	situ	271:274	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	0	73	theme	Anti-Proliferative	100:117	arg1	Activity					119:126	Effective Anti-Proliferative Activity	90:126	Effective Anti-Proliferative Activity against Tongue Carcinoma	90:151	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	1	74	theme	composite	326:334	arg1	FCCCD					344:348	FCCCD	344:348	FCCCD	344:348	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	74	theme	composite	326:334	arg1	design					336:341	a face-centered central composite design	302:341	a face-centered central composite design (FCCCD)	302:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	8	75	theme	polymer	1585:1591	arg1	interaction					1605:1615	polymer synergistic interaction	1585:1615	polymer synergistic interaction	1585:1615	Enhanced biological activity of SIM was observed due to QRC enabling promising drug and polymer synergistic interaction.					
32283782	1	76	theme	Simvastatin	217:227	arg1	NPs					250:252	NPs	250:252	NPs	250:252	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	1	76	theme	Simvastatin	217:227	arg1	nanoparticles					235:247	optimized chitosan-coated Simvastatin (SIM) nanoparticles	191:247	optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD)	191:349	The goal of this study is to develop optimized chitosan-coated Simvastatin (SIM) nanoparticles (NPs) loaded in an in situ gel (ISG) formulation via a face-centered central composite design (FCCCD).					
32283782	7	77	theme	protein	1481:1487	arg1	levels					1489:1494	the tumor suppressor protein levels	1460:1494	the tumor suppressor protein levels	1460:1494	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	7	78	theme	tumor	1464:1468	arg1	levels					1489:1494	the tumor suppressor protein levels	1460:1494	the tumor suppressor protein levels	1460:1494	ISG formulation showed a considerable increase in apoptosis occurrence through caspase-3 mediation and it also enhanced the tumor suppressor protein levels.					
32283782	3	79	from	levels	517:522	arg1	concentrations					446:459	The concentrations	442:459	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point	442:628	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	6	80	theme	h	1233:1233	arg1	end					1222:1224	the end	1218:1224	the end of 168 h	1218:1233	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	6	81	from	ISG	1170:1172	arg1	release					1139:1145	4 °C. Drug release	1128:1145	4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1128:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	4	82	theme	NP-loaded	900:908	arg1	ISG					910:912	SIM-QRC NP-loaded ISG	892:912	SIM-QRC NP-loaded ISG	892:912	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	0	83	theme	Chitosan-Coated	24:38	arg1	Nanoparticles					52:64	Chitosan-Coated Simvastatin Nanoparticles	24:64	Chitosan-Coated Simvastatin Nanoparticles	24:64	In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma.					
32283782	9	84	theme	proposed	1622:1629	arg1	formulation					1631:1641	The proposed formulation	1618:1641	The proposed formulation	1618:1641	The proposed formulation can provide a breakthrough in localized therapy, overcoming the potential drawbacks of systemic chemotherapy for tongue carcinoma.					
32283782	6	85	from	release	1139:1145	arg1	stability					1115:1123	better stability	1108:1123	better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM	1108:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	4	86	theme	formulation	866:876	arg1	formulation					866:876	optimum formulation	858:876	optimum formulation	858:876	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	4	86	theme	formulation	866:876	arg1	prerequisites					841:853	the prerequisites	837:853	the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG	837:912	Based on the desirability approach, a formulation containing poloxamer 188 0.24% and chitosan 0.43% renders the prerequisites of optimum formulation for preparing SIM-QRC NP-loaded ISG.					
32283782	3	87	theme	poloxamer	464:472	arg1	concentrations					446:459	The concentrations	442:459	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point	442:628	The concentrations of poloxamer 188 (A) and chitosan (B) at five different levels, plus/minus alpha (+1.414 and -1.414: axial points), plus/minus 1 (factorial points) and the center point were optimized for particle size (PS-Y1), entrapment efficacy (EE-Y2) and stability index (SI-Y3).					
32283782	6	88	theme	SIM	1251:1253	arg1	SIM					1251:1253	SIM	1251:1253	SIM	1251:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32283782	6	88	theme	SIM	1251:1253	arg1	%					1246:1246	only 65.12%	1236:1246	only 65.12% of SIM	1236:1253	The optimized formulation indicated the highest EE 79.67% and better stability at 4 °C. Drug release from SIM-QRC NP-loaded ISG was slower to plateau by up to 96 h and, at the end of 168 h, only 65.12% of SIM was released in a more controlled manner in comparison to SIM-QRC NPs and plain SIM.					
32718649	2	0	theme	Fe3O4	342:346	arg1	nanoparticles					348:360	Fe3O4 nanoparticles	342:360	Fe3O4 nanoparticles	342:360	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	6	1	from	nature	901:906	arg1	process					875:881	the adsorption process	860:881	the adsorption process	860:881	The thermodynamic studies indicated that the adsorption process was exothermic in nature.					
32718649	6	1	from	nature	901:906	arg1	exothermic					887:896	exothermic	887:896	exothermic	887:896	The thermodynamic studies indicated that the adsorption process was exothermic in nature.					
32718649	1	2	from	medium	308:313	arg1	removal					265:271	the removal	261:271	the removal of methyl orange (MO) from aqueous medium	261:313	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	7	3	theme	recycle	959:965	arg1	ability					967:973	satisfactory recycle ability	946:973	satisfactory recycle ability	946:973	Furthermore, the adsorbent exhibited satisfactory recycle ability.					
32718649	6	4	theme	thermodynamic	823:835	arg1	studies					837:843	The thermodynamic studies	819:843	The thermodynamic studies	819:843	The thermodynamic studies indicated that the adsorption process was exothermic in nature.					
32718649	8	5	theme	wastewater	1166:1175	arg1	treatment					1177:1185	wastewater treatment	1166:1185	wastewater treatment	1166:1185	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
32718649	8	6	theme	application	1042:1052	arg1	scope					1054:1058	the application scope	1038:1058	the application scope of kaolin	1038:1068	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
32718649	1	7	dep	Chitosan	130:137	arg1	composite					182:190	modified magnetic kaolin (CS/kaolin/Fe3O4) composite	139:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite	130:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	0	8	theme	efficient	94:102	arg1	removal					104:110	efficient removal	94:110	efficient removal of methyl orange	94:127	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	3	9	theme	adsorption	526:535	arg1	sites					537:541	adsorption sites	526:541	adsorption sites for anionic species	526:561	With the modification of CS, adsorption sites for anionic species were introduced onto the adsorbent.					
32718649	4	10	theme	adsorption	690:699	arg1	capacity					701:708	a high saturated adsorption capacity	673:708	a high saturated adsorption capacity of 349.7 mg/g	673:722	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	0	11	theme	methyl	115:120	arg1	orange					122:127	methyl orange	115:127	methyl orange	115:127	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	7	12	theme	satisfactory	946:957	arg1	ability					967:973	satisfactory recycle ability	946:973	satisfactory recycle ability	946:973	Furthermore, the adsorbent exhibited satisfactory recycle ability.					
32718649	2	13	theme	kaolin	384:389	arg1	layer					391:395	the kaolin layer	380:395	the kaolin layer	380:395	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	6	14	theme	adsorption	864:873	arg1	process					875:881	the adsorption process	860:881	the adsorption process	860:881	The thermodynamic studies indicated that the adsorption process was exothermic in nature.					
32718649	6	14	theme	adsorption	864:873	arg1	exothermic					887:896	exothermic	887:896	exothermic	887:896	The thermodynamic studies indicated that the adsorption process was exothermic in nature.					
32718649	2	15	theme	kaolin	456:461	arg1	layer					463:467	the kaolin layer	452:467	the kaolin layer	452:467	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	3	16	theme	CS	522:523	arg1	modification					506:517	the modification	502:517	the modification of CS	502:523	With the modification of CS, adsorption sites for anionic species were introduced onto the adsorbent.					
32718649	5	17	theme	adsorption	729:738	arg1	process					740:746	The adsorption process	725:746	The adsorption process	725:746	The adsorption process was controlled by film diffusion and well described by Langmuir model.					
32718649	3	18	theme	anionic	547:553	arg1	species					555:561	anionic species	547:561	anionic species	547:561	With the modification of CS, adsorption sites for anionic species were introduced onto the adsorbent.					
32718649	0	19	theme	Facile	0:5	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of chitosan	0:29	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	1	20	theme	methyl	276:281	arg1	MO					291:292	MO	291:292	MO	291:292	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	1	20	theme	methyl	276:281	arg1	orange					283:288	methyl orange	276:288	methyl orange (MO)	276:293	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	8	21	theme	kaolin	1063:1068	arg1	scope					1054:1058	the application scope	1038:1058	the application scope of kaolin	1038:1068	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
32718649	0	22	theme	chitosan	22:29	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of chitosan	0:29	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	1	23	theme	orange	283:288	arg1	removal					265:271	the removal	261:271	the removal of methyl orange (MO) from aqueous medium	261:313	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	8	24	theme	species	1081:1087	arg1	removal					1089:1095	anionic species removal	1073:1095	anionic species removal	1073:1095	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
32718649	2	25	theme	growth	408:413	arg1	method					415:420	in-situ growth method	400:420	in-situ growth method	400:420	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	0	26	theme	orange	122:127	arg1	removal					104:110	efficient removal	94:110	efficient removal of methyl orange	94:127	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	4	27	theme	mg/g	719:722	arg1	capacity					701:708	a high saturated adsorption capacity	673:708	a high saturated adsorption capacity of 349.7 mg/g	673:722	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	2	28	theme	in-situ	400:406	arg1	method					415:420	in-situ growth method	400:420	in-situ growth method	400:420	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	0	29	theme	magnetic	40:47	arg1	kaolin					49:54	magnetic kaolin	40:54	magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange	40:127	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	4	30	theme	high	675:678	arg1	capacity					701:708	a high saturated adsorption capacity	673:708	a high saturated adsorption capacity of 349.7 mg/g	673:722	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	8	31	theme	anionic	1073:1079	arg1	removal					1089:1095	anionic species removal	1073:1095	anionic species removal	1073:1095	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
32718649	1	32	theme	facile	210:215	arg1	method					241:246	a facile one-pot coprecipitation method	208:246	a facile one-pot coprecipitation method	208:246	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	1	33	theme	one-pot	217:223	arg1	method					241:246	a facile one-pot coprecipitation method	208:246	a facile one-pot coprecipitation method	208:246	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	5	34	theme	film	766:769	arg1	diffusion					771:779	film diffusion	766:779	film diffusion	766:779	The adsorption process was controlled by film diffusion and well described by Langmuir model.					
32718649	8	35	theme	CS/kaolin/Fe3O4	1105:1119	arg1	composite					1121:1129	the CS/kaolin/Fe3O4 composite	1101:1129	the CS/kaolin/Fe3O4 composite	1101:1129	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
32718649	0	36	theme	one-pot	59:65	arg1	method					83:88	one-pot coprecipitation method	59:88	one-pot coprecipitation method	59:88	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	4	37	theme	saturated	680:688	arg1	capacity					701:708	a high saturated adsorption capacity	673:708	a high saturated adsorption capacity of 349.7 mg/g	673:722	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	1	38	theme	aqueous	300:306	arg1	medium					308:313	aqueous medium	300:313	aqueous medium	300:313	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	1	39	used	used	252:255	arg2	Chitosan					130:137	Chitosan	130:137	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite	130:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	2	40	theme	alkaline	322:329	arg1	condition					331:339	alkaline condition	322:339	alkaline condition	322:339	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	6	41	from	exothermic	887:896	arg1	nature					901:906	nature	901:906	nature	901:906	The thermodynamic studies indicated that the adsorption process was exothermic in nature.					
32718649	4	42	theme	MO	659:660	arg1	MO					659:660	MO	659:660	MO	659:660	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	4	42	theme	MO	659:660	arg1	%					654:654	more than 94 %	641:654	more than 94 % of MO	641:660	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	1	43	theme	coprecipitation	225:239	arg1	method					241:246	a facile one-pot coprecipitation method	208:246	a facile one-pot coprecipitation method	208:246	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	1	44	theme	modified	139:146	arg1	composite					182:190	modified magnetic kaolin (CS/kaolin/Fe3O4) composite	139:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite	130:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	0	45	theme	coprecipitation	67:81	arg1	method					83:88	one-pot coprecipitation method	59:88	one-pot coprecipitation method	59:88	Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.					
32718649	5	46	theme	Langmuir	803:810	arg1	model					812:816	Langmuir model	803:816	Langmuir model	803:816	The adsorption process was controlled by film diffusion and well described by Langmuir model.					
32718649	1	47	theme	magnetic	148:155	arg1	kaolin					157:162	magnetic kaolin	148:162	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite	130:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	1	47	theme	magnetic	148:155	arg1	CS/kaolin/Fe3O4					165:179	CS/kaolin/Fe3O4	165:179	CS/kaolin/Fe3O4	165:179	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	4	48	theme	prepared	603:610	arg1	CS/kaolin/Fe3O4					612:626	The prepared CS/kaolin/Fe3O4	599:626	The prepared CS/kaolin/Fe3O4	599:626	The prepared CS/kaolin/Fe3O4 could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g.					
32718649	2	49	theme	pH-precipitation	472:487	arg1	method					489:494	pH-precipitation method	472:494	pH-precipitation method	472:494	Under alkaline condition, Fe3O4 nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method.					
32718649	1	50	theme	kaolin	157:162	arg1	composite					182:190	modified magnetic kaolin (CS/kaolin/Fe3O4) composite	139:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite	130:190	Chitosan modified magnetic kaolin (CS/kaolin/Fe3O4) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium.					
32718649	8	51	with	modification	1007:1018	arg1	CS					1025:1026	CS	1025:1026	CS	1025:1026	The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/Fe3O4 composite could be a promising adsorbent for wastewater treatment.					
34808357	2	0	theme	amino	757:761	arg1	dl-methionine					779:791	dl-methionine	779:791	dl-methionine	779:791	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	0	theme	amino	757:761	arg1	acids					763:767	possible immune modulating amino acids	730:767	possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet)	730:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	0	theme	amino	757:761	arg1	l-threonine					804:814	l-threonine	804:814	l-threonine	804:814	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	0	theme	amino	757:761	arg1	taurine					820:826	taurine	820:826	taurine (Amino acid diet)	820:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	0	theme	amino	757:761	arg1	l-lysine					794:801	l-lysine	794:801	l-lysine	794:801	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	5	1	theme	individual	1235:1244	arg1	suspension					1256:1265	the individual leukocyte suspension	1231:1265	the individual leukocyte suspension from all fish	1231:1279	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	2	2	theme	immune	739:744	arg1	dl-methionine					779:791	dl-methionine	779:791	dl-methionine	779:791	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	2	theme	immune	739:744	arg1	acids					763:767	possible immune modulating amino acids	730:767	possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet)	730:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	2	theme	immune	739:744	arg1	l-threonine					804:814	l-threonine	804:814	l-threonine	804:814	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	2	theme	immune	739:744	arg1	taurine					820:826	taurine	820:826	taurine (Amino acid diet)	820:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	2	theme	immune	739:744	arg1	l-lysine					794:801	l-lysine	794:801	l-lysine	794:801	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	4	3	theme	ongoing	1135:1141	arg1	infection					1146:1154	ongoing PD infection	1135:1154	ongoing PD infection	1135:1154	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	5	4	theme	inflammation	1208:1219	arg1	in vitro					1221:1228	bacterial or virus induced inflammation in vitro	1181:1228	bacterial or virus induced inflammation in vitro	1181:1228	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	2	5	theme	vegetable	538:546	arg1	oils					548:551	vegetable oils	538:551	vegetable oils	538:551	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	7	6	theme	gene	1566:1569	arg1	transcription					1571:1583	overall gene transcription	1558:1583	overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase)	1558:1633	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	2	7	theme	natural	642:648	arg1	diet					650:653	the natural diet	638:653	the natural diet of wild salmon	638:668	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	9	8	theme	Fatty	1874:1878	arg1	acid					1880:1883	Fatty acid	1874:1883	Fatty acid	1874:1883	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	1	9	theme	freshwater	178:187	arg1	impact					195:200	freshwater diets impact	178:200	freshwater diets impact on immunity in Atlantic salmon	178:231	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	5	10	from	fish	1276:1279	arg1	suspension					1256:1265	the individual leukocyte suspension	1231:1265	the individual leukocyte suspension from all fish	1231:1279	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	10	11	theme	PIC	2012:2014	arg1	challenge					2016:2024	PIC challenge	2012:2024	PIC challenge in all diet groups	2012:2043	In seawater post smolts, Mx and viperin responded similarly to PIC challenge in all diet groups.					
34808357	0	12	from	robustness	83:92	arg1	salmon					106:111	Atlantic salmon	97:111	Atlantic salmon post smolts	97:123	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	2	13	theme	salmon	663:668	arg1	diet					650:653	the natural diet	638:653	the natural diet of wild salmon	638:668	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	9	14	theme	acid	1895:1898	arg1	diet					1900:1903	Amino acid diet	1889:1903	Amino acid diet	1889:1903	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	8	15	theme	acid	1664:1667	arg1	diet					1669:1672	the Amino acid diet	1654:1672	the Amino acid diet	1654:1672	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	10	16	theme	diet	2033:2036	arg1	groups					2038:2043	all diet groups	2029:2043	all diet groups	2029:2043	In seawater post smolts, Mx and viperin responded similarly to PIC challenge in all diet groups.					
34808357	4	17	theme	post	1027:1030	arg1	smolts					1032:1037	post smolts	1027:1037	post smolts one and six weeks after transfer to seawater	1027:1082	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	7	18	theme	Fatty	1527:1531	arg1	diet					1538:1541	the Standard or Fatty acid diet	1511:1541	the Standard or Fatty acid diet	1511:1541	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	9	19	theme	Standard	1928:1935	arg1	group					1942:1946	the Standard diet group	1924:1946	the Standard diet group	1924:1946	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	8	20	theme	gene	1701:1704	arg1	transcription					1706:1718	the LPS induced gene transcription	1685:1718	the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ	1685:1750	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	1	21	theme	current	141:147	arg1	study					149:153	the current study	137:153	the current study	137:153	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	7	22	theme	Standard	1515:1522	arg1	diet					1538:1541	the Standard or Fatty acid diet	1511:1541	the Standard or Fatty acid diet	1511:1541	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	2	23	theme	similar	436:442	arg1	diet					711:714	diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet	431:714	A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet	429:714	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	24	theme	Amino	829:833	arg1	taurine					820:826	taurine	820:826	taurine (Amino acid diet)	820:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	24	theme	Amino	829:833	arg1	diet					840:843	Amino acid diet	829:843	Amino acid diet	829:843	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	25	theme	aquatic	620:626	arg1	insects					628:634	aquatic insects	620:634	aquatic insects	620:634	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	6	26	theme	types	1456:1460	arg1	transcription					1431:1443	the transcription	1427:1443	the transcription of several types of genes	1427:1469	The transfer of smolts from fresh-to seawater changed the transcription of several types of genes.					
34808357	2	27	theme	freshwater	480:489	arg1	diets					491:495	commercial salmon freshwater diets	462:495	commercial salmon freshwater diets (Standard diet)	462:511	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	27	theme	freshwater	480:489	arg1	diet					507:510	Standard diet	498:510	Standard diet	498:510	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	0	28	theme	Atlantic	97:104	arg1	salmon					106:111	Atlantic salmon	97:111	Atlantic salmon post smolts	97:123	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	3	29	theme	commercial	899:908	arg1	diet					910:913	the same commercial diet	890:913	the same commercial diet	890:913	After seawater transfer, all fish were fed the same commercial diet.					
34808357	2	30	theme	commercial	462:471	arg1	diets					491:495	commercial salmon freshwater diets	462:495	commercial salmon freshwater diets (Standard diet)	462:511	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	30	theme	commercial	462:471	arg1	diet					507:510	Standard diet	498:510	Standard diet	498:510	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	31	theme	rapeseed	554:561	arg1	diet					521:524	A diet	519:524	(ii) A diet composed of vegetable oils (rapeseed	514:561	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	32	dep	mimicking	587:595	arg1	iii					704:706	iii	704:706	iii	704:706	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	1	33	theme	pancreas	345:352	arg1	PD					363:364	PD	363:364	PD	363:364	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	1	33	theme	pancreas	345:352	arg1	disease					354:360	pancreas disease	345:360	pancreas disease (PD) infection	345:375	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	2	34	theme	linseed	573:579	arg1	oils					581:584	linseed oils	573:584	linseed oils	573:584	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	35	theme	freshwater	393:402	arg1	diets					404:408	Three specific freshwater diets	378:408	Three specific freshwater diets	378:408	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	8	36	theme	INF-γ	1746:1750	arg1	transcription					1706:1718	the LPS induced gene transcription	1685:1718	the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ	1685:1750	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	0	37	theme	boosted	35:41	arg1	immunity					43:50	boosted immunity	35:50	boosted immunity	35:50	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	0	38	theme	disease	65:71	arg1	robustness					83:92	pancreas disease infection robustness	56:92	pancreas disease infection robustness	56:92	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	7	39	dep	transcription	1571:1583	arg1	INF-γ					1599:1603	INF-γ	1599:1603	INF-γ	1599:1603	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	7	39	dep	transcription	1571:1583	arg1	MGAT2					1618:1622	MGAT2	1618:1622	MGAT2	1618:1622	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	7	39	dep	transcription	1571:1583	arg1	catalase					1625:1632	catalase	1625:1632	catalase	1625:1632	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	7	39	dep	transcription	1571:1583	arg1	IL-1β					1586:1590	IL-1β	1586:1590	IL-1β	1586:1590	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	7	39	dep	transcription	1571:1583	arg1	cox2					1606:1609	cox2	1606:1609	cox2	1606:1609	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	7	39	dep	transcription	1571:1583	arg1	cd36					1612:1615	cd36	1612:1615	cd36	1612:1615	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	7	39	dep	transcription	1571:1583	arg1	CD83					1593:1596	CD83	1593:1596	CD83	1593:1596	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	5	40	theme	polyinosinic	1328:1339	arg1	acid					1341:1344	polyinosinic acid	1328:1344	polyinosinic acid	1328:1344	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	5	41	theme	induced	1200:1206	arg1	in vitro					1221:1228	bacterial or virus induced inflammation in vitro	1181:1228	bacterial or virus induced inflammation in vitro	1181:1228	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	2	42	theme	acid	691:694	arg1	diet					696:699	Fatty acid diet	685:699	Fatty acid diet	685:699	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	42	theme	acid	691:694	arg1	freshwater					673:682	freshwater	673:682	freshwater (Fatty acid diet)	673:700	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	43	dep	enriched	716:723	arg1	i					426:426	i	426:426	i	426:426	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	10	44	from	challenge	2016:2024	arg1	groups					2038:2043	all diet groups	2029:2043	all diet groups	2029:2043	In seawater post smolts, Mx and viperin responded similarly to PIC challenge in all diet groups.					
34808357	4	45	theme	PD	1143:1144	arg1	infection					1146:1154	ongoing PD infection	1135:1154	ongoing PD infection	1135:1154	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	9	46	theme	PIC	1789:1791	arg1	leukocytes					1804:1813	PIC stimulated leukocytes	1789:1813	PIC stimulated leukocytes	1789:1813	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	2	47	theme	modulating	746:755	arg1	dl-methionine					779:791	dl-methionine	779:791	dl-methionine	779:791	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	47	theme	modulating	746:755	arg1	acids					763:767	possible immune modulating amino acids	730:767	possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet)	730:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	47	theme	modulating	746:755	arg1	l-threonine					804:814	l-threonine	804:814	l-threonine	804:814	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	47	theme	modulating	746:755	arg1	taurine					820:826	taurine	820:826	taurine (Amino acid diet)	820:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	47	theme	modulating	746:755	arg1	l-lysine					794:801	l-lysine	794:801	l-lysine	794:801	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	9	48	theme	Mx	1852:1853	arg1	level					1843:1847	higher transcription level	1822:1847	higher transcription level of Mx and viperin	1822:1865	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	5	49	theme	leukocyte	1246:1254	arg1	suspension					1256:1265	the individual leukocyte suspension	1231:1265	the individual leukocyte suspension from all fish	1231:1279	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	10	50	theme	post	1961:1964	arg1	smolts					1966:1971	seawater post smolts	1952:1971	seawater post smolts	1952:1971	In seawater post smolts, Mx and viperin responded similarly to PIC challenge in all diet groups.					
34808357	3	51	theme	seawater	853:860	arg1	transfer					862:869	seawater transfer	853:869	seawater transfer	853:869	After seawater transfer, all fish were fed the same commercial diet.					
34808357	2	52	theme	wild	658:661	arg1	salmon					663:668	wild salmon	658:668	wild salmon	658:668	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	53	theme	possible	730:737	arg1	dl-methionine					779:791	dl-methionine	779:791	dl-methionine	779:791	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	53	theme	possible	730:737	arg1	acids					763:767	possible immune modulating amino acids	730:767	possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet)	730:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	53	theme	possible	730:737	arg1	l-threonine					804:814	l-threonine	804:814	l-threonine	804:814	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	53	theme	possible	730:737	arg1	taurine					820:826	taurine	820:826	taurine (Amino acid diet)	820:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	53	theme	possible	730:737	arg1	l-lysine					794:801	l-lysine	794:801	l-lysine	794:801	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	9	54	theme	viperin	1859:1865	arg1	level					1843:1847	higher transcription level	1822:1847	higher transcription level of Mx and viperin	1822:1865	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	7	55	theme	overall	1558:1564	arg1	transcription					1571:1583	overall gene transcription	1558:1583	overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase)	1558:1633	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	4	56	theme	right	987:991	arg1	smolts					980:985	smolts	980:985	smolts right before transfer to seawater	980:1019	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	2	57	from	composition	605:615	arg1	insects					628:634	aquatic insects	620:634	aquatic insects	620:634	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	9	58	theme	acid	1880:1883	arg1	groups					1905:1910	the Fatty acid and Amino acid diet groups	1870:1910	the Fatty acid and Amino acid diet groups	1870:1910	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	8	59	theme	Amino	1658:1662	arg1	diet					1669:1672	the Amino acid diet	1654:1672	the Amino acid diet	1654:1672	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	1	60	theme	diets	189:193	arg1	impact					195:200	freshwater diets impact	178:200	freshwater diets impact on immunity in Atlantic salmon	178:231	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	2	61	theme	Standard	498:505	arg1	diets					491:495	commercial salmon freshwater diets	462:495	commercial salmon freshwater diets (Standard diet)	462:511	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	61	theme	Standard	498:505	arg1	diet					507:510	Standard diet	498:510	Standard diet	498:510	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	9	62	theme	Amino	1889:1893	arg1	diet					1900:1903	Amino acid diet	1889:1903	Amino acid diet	1889:1903	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	5	63	dep	stimulated	1286:1295	arg1	PIC					1367:1369	PIC	1367:1369	PIC	1367:1369	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	5	63	dep	stimulated	1286:1295	arg1	acid					1361:1364	polycytidylic acid	1347:1364	polycytidylic acid (PIC)	1347:1370	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	11	64	attach	isolated	2070:2077	arg1	fish					2096:2099	PD infected fish	2084:2099	PD infected fish	2084:2099	Furthermore, leukocytes isolated from PD infected fish, continued responding to PIC, regardless of freshwater diet.					
34808357	11	64	attach	isolated	2070:2077	arg2	leukocytes					2059:2068	leukocytes	2059:2068	leukocytes	2059:2068	Furthermore, leukocytes isolated from PD infected fish, continued responding to PIC, regardless of freshwater diet.					
34808357	9	65	theme	diet	1900:1903	arg1	groups					1905:1910	the Fatty acid and Amino acid diet groups	1870:1910	the Fatty acid and Amino acid diet groups	1870:1910	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	6	66	theme	smolts	1389:1394	arg1	transfer					1377:1384	The transfer	1373:1384	The transfer of smolts from fresh-to seawater	1373:1417	The transfer of smolts from fresh-to seawater changed the transcription of several types of genes.					
34808357	2	67	dep	diet	521:524	arg1	ii					515:516	ii	515:516	ii	515:516	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	68	theme	fat	601:603	arg1	composition					605:615	the fat composition	597:615	the fat composition in aquatic insects	597:634	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	8	69	theme	induced	1693:1699	arg1	transcription					1706:1718	the LPS induced gene transcription	1685:1718	the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ	1685:1750	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	1	70	from	impact	195:200	arg1	salmon					226:231	Atlantic salmon	217:231	Atlantic salmon	217:231	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	1	70	from	impact	195:200	arg1	immunity					205:212	immunity	205:212	immunity	205:212	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	6	71	theme	genes	1465:1469	arg1	types					1456:1460	several types	1448:1460	several types of genes	1448:1469	The transfer of smolts from fresh-to seawater changed the transcription of several types of genes.					
34808357	0	72	theme	infection	73:81	arg1	robustness					83:92	pancreas disease infection robustness	56:92	pancreas disease infection robustness	56:92	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	6	73	from	seawater	1410:1417	arg1	transfer					1377:1384	The transfer	1373:1384	The transfer of smolts from fresh-to seawater	1373:1417	The transfer of smolts from fresh-to seawater changed the transcription of several types of genes.					
34808357	7	74	theme	acid	1533:1536	arg1	diet					1538:1541	the Standard or Fatty acid diet	1511:1541	the Standard or Fatty acid diet	1511:1541	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	2	75	theme	acid	835:838	arg1	taurine					820:826	taurine	820:826	taurine (Amino acid diet)	820:844	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	75	theme	acid	835:838	arg1	diet					840:843	Amino acid diet	829:843	Amino acid diet	829:843	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	7	76	from	fish	1502:1505	arg1	isolates					1488:1495	isolates	1488:1495	isolates from fish fed the Standard or Fatty acid diet in freshwater	1488:1555	Particularly in isolates from fish fed the Standard or Fatty acid diet in freshwater, overall gene transcription (IL-1β, CD83, INF-γ, cox2, cd36, MGAT2, catalase) declined.					
34808357	9	77	theme	diet	1937:1940	arg1	group					1942:1946	the Standard diet group	1924:1946	the Standard diet group	1924:1946	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	8	78	theme	IL-1β	1723:1727	arg1	transcription					1706:1718	the LPS induced gene transcription	1685:1718	the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ	1685:1750	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	1	79	theme	seawater	313:320	arg1	stage					322:326	the seawater stage	309:326	the seawater stage with and without pancreas disease (PD) infection	309:375	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	1	80	theme	study	149:153	arg1	aim					130:132	The aim	126:132	The aim of the current study	126:153	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	11	81	theme	freshwater	2145:2154	arg1	diet					2156:2159	freshwater diet	2145:2159	freshwater diet	2145:2159	Furthermore, leukocytes isolated from PD infected fish, continued responding to PIC, regardless of freshwater diet.					
34808357	11	82	dep	infected	2087:2094	arg1	PD					2084:2085	PD	2084:2085	PD	2084:2085	Furthermore, leukocytes isolated from PD infected fish, continued responding to PIC, regardless of freshwater diet.					
34808357	8	83	theme	CD83	1730:1733	arg1	transcription					1706:1718	the LPS induced gene transcription	1685:1718	the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ	1685:1750	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	3	84	theme	same	894:897	arg1	diet					910:913	the same commercial diet	890:913	the same commercial diet	890:913	After seawater transfer, all fish were fed the same commercial diet.					
34808357	6	85	theme	several	1448:1454	arg1	types					1456:1460	several types	1448:1460	several types of genes	1448:1469	The transfer of smolts from fresh-to seawater changed the transcription of several types of genes.					
34808357	2	86	theme	salmon	473:478	arg1	diets					491:495	commercial salmon freshwater diets	462:495	commercial salmon freshwater diets (Standard diet)	462:511	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	86	theme	salmon	473:478	arg1	diet					507:510	Standard diet	498:510	Standard diet	498:510	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	8	87	theme	Cox2	1736:1739	arg1	transcription					1706:1718	the LPS induced gene transcription	1685:1718	the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ	1685:1750	However, the Amino acid diet stimulated the LPS induced gene transcription of IL-1β, CD83, Cox2, and INF-γ at this stage.					
34808357	0	88	from	immunity	43:50	arg1	salmon					106:111	Atlantic salmon	97:111	Atlantic salmon post smolts	97:123	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	10	89	theme	seawater	1952:1959	arg1	smolts					1966:1971	seawater post smolts	1952:1971	seawater post smolts	1952:1971	In seawater post smolts, Mx and viperin responded similarly to PIC challenge in all diet groups.					
34808357	0	90	theme	freshwater	10:19	arg1	diets					21:25	freshwater diets	10:25	freshwater diets	10:25	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	1	91	theme	disease	354:360	arg1	infection					367:375	pancreas disease (PD) infection	345:375	pancreas disease (PD) infection	345:375	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	9	92	theme	higher	1822:1827	arg1	level					1843:1847	higher transcription level	1822:1847	higher transcription level of Mx and viperin	1822:1865	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	6	93	theme	fresh-to	1401:1408	arg1	seawater					1410:1417	fresh-to seawater	1401:1417	fresh-to seawater	1401:1417	The transfer of smolts from fresh-to seawater changed the transcription of several types of genes.					
34808357	5	94	theme	polycytidylic	1347:1359	arg1	PIC					1367:1369	PIC	1367:1369	PIC	1367:1369	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	5	94	theme	polycytidylic	1347:1359	arg1	acid					1361:1364	polycytidylic acid	1347:1364	polycytidylic acid (PIC)	1347:1370	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	9	95	theme	freshwater	1770:1779	arg1	smolts					1781:1786	freshwater smolts	1770:1786	freshwater smolts	1770:1786	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	2	96	theme	specific	384:391	arg1	diets					404:408	Three specific freshwater diets	378:408	Three specific freshwater diets	378:408	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	4	97	from	smolts	1099:1104	arg1	seawater					1109:1116	seawater	1109:1116	seawater	1109:1116	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	5	98	theme	bacterial	1181:1189	arg1	in vitro					1221:1228	bacterial or virus induced inflammation in vitro	1181:1228	bacterial or virus induced inflammation in vitro	1181:1228	In addition, to provoke bacterial or virus induced inflammation in vitro, the individual leukocyte suspension from all fish were stimulated by lipopolysaccharide (LPS) or polyinosinic acid: polycytidylic acid (PIC).					
34808357	0	99	theme	pancreas	56:63	arg1	robustness					83:92	pancreas disease infection robustness	56:92	pancreas disease infection robustness	56:92	Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts.					
34808357	1	100	theme	post	290:293	arg1	smolts					295:300	post smolts	290:300	post smolts	290:300	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	9	101	theme	transcription	1829:1841	arg1	level					1843:1847	higher transcription level	1822:1847	higher transcription level of Mx and viperin	1822:1865	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	9	102	theme	stimulated	1793:1802	arg1	leukocytes					1804:1813	PIC stimulated leukocytes	1789:1813	PIC stimulated leukocytes	1789:1813	In freshwater smolts, PIC stimulated leukocytes showed higher transcription level of Mx and viperin in the Fatty acid and Amino acid diet groups compared to the Standard diet group.					
34808357	4	103	dep	weeks	1051:1055	arg1	transfer					1063:1070	transfer	1063:1070	transfer to seawater	1063:1082	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	11	104	theme	infected	2087:2094	arg1	fish					2096:2099	PD infected fish	2084:2099	PD infected fish	2084:2099	Furthermore, leukocytes isolated from PD infected fish, continued responding to PIC, regardless of freshwater diet.					
34808357	4	105	theme	Head	916:919	arg1	kidneys					921:927	Head kidneys	916:927	Head kidneys	916:927	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	4	106	theme	post	1094:1097	arg1	smolts					1099:1104	post smolts	1094:1104	post smolts in seawater	1094:1116	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
34808357	1	107	theme	Atlantic	217:224	arg1	salmon					226:231	Atlantic salmon	217:231	Atlantic salmon	217:231	The aim of the current study was to investigate how freshwater diets impact on immunity in Atlantic salmon smolts in freshwater, during transfer to seawater and in post smolts during the seawater stage with and without pancreas disease (PD) infection.					
34808357	2	108	theme	Fatty	685:689	arg1	diet					696:699	Fatty acid diet	685:699	Fatty acid diet	685:699	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	2	108	theme	Fatty	685:689	arg1	freshwater					673:682	freshwater	673:682	freshwater (Fatty acid diet)	673:700	Three specific freshwater diets were prepared: (i) A diet similar in composition to commercial salmon freshwater diets (Standard diet); (ii) A diet composed of vegetable oils (rapeseed, palm and linseed oils) mimicking the fat composition in aquatic insects - the natural diet of wild salmon in freshwater (Fatty acid diet); (iii) A diet enriched with possible immune modulating amino acids including dl-methionine, l-lysine, l-threonine and taurine (Amino acid diet).					
34808357	4	109	theme	infection	1146:1154	arg1	weeks					1126:1130	8 weeks	1124:1130	8 weeks of ongoing PD infection	1124:1154	Head kidneys were extracted, and their leukocytes isolated from smolts right before transfer to seawater, from post smolts one and six weeks after transfer to seawater, and from post smolts in seawater after 8 weeks of ongoing PD infection.					
32079210	11	0	theme	health	1232:1237	arg1	claims					1239:1244	all approved health claims	1219:1244	all approved health claims referred to wheat	1219:1262	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	1	theme	bran	1320:1323	arg1	fraction					1325:1332	bran fraction	1320:1332	bran fraction	1320:1332	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	1	theme	bran	1320:1323	arg1	samples					1284:1290	all analyzed samples	1271:1290	all analyzed samples	1271:1290	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	4	2	theme	bioactive	480:488	arg1	compounds					490:498	other bioactive compounds	474:498	other bioactive compounds (phenolics and tocopherols)	474:526	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	4	2	theme	bioactive	480:488	arg1	approach					555:562	a first approach	547:562	a first approach to their phytochemical composition in the analyzed wheat varieties	547:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	0	3	theme	Functional	59:68	arg1	Ingredients					70:80	Functional Ingredients	59:80	Functional Ingredients	59:80	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	1	4	theme	human	131:135	arg1	nutrition					137:145	human nutrition	131:145	human nutrition	131:145	Wheat is an important cereal with a key role in human nutrition.					
32079210	4	5	from	composition	587:597	arg1	varieties					621:629	the analyzed wheat varieties	602:629	the analyzed wheat varieties	602:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	4	6	theme	other	474:478	arg1	compounds					490:498	other bioactive compounds	474:498	other bioactive compounds (phenolics and tocopherols)	474:526	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	4	6	theme	other	474:478	arg1	approach					555:562	a first approach	547:562	a first approach to their phytochemical composition in the analyzed wheat varieties	547:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	11	7	theme	health	1352:1357	arg1	ingredients					1383:1393	functional ingredients	1372:1393	functional ingredients	1372:1393	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	7	theme	health	1352:1357	arg1	foods					1409:1413	functional foods	1398:1413	functional foods	1398:1413	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	7	theme	health	1352:1357	arg1	benefits					1359:1366	potential health benefits	1342:1366	potential health benefits	1342:1366	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	8	8	contain	contained	1024:1032	arg2	compounds					1057:1065	higher total phenolics compounds	1034:1065	higher total phenolics compounds	1034:1065	Whole flours contained higher total phenolics compounds.					
32079210	8	8	contain	contained	1024:1032	arg1	flours					1017:1022	Whole flours	1011:1022	Whole flours	1011:1022	Whole flours contained higher total phenolics compounds.					
32079210	2	9	theme	ssp	238:240	arg1	DF					178:179	DF	178:179	DF	178:179	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	2	9	theme	ssp	238:240	arg1	arabinoxylans					186:198	arabinoxylans	186:198	arabinoxylans of different durum (Triticum turgidum ssp	186:240	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	2	9	theme	ssp	238:240	arg1	fiber					171:175	dietary fiber	163:175	dietary fiber (DF)	163:180	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	3	10	theme	wheat	286:290	arg1	flours					292:297	Durum L.) and bread (Triticum aestivum L.) wheat flours	243:297	Durum L.) and bread (Triticum aestivum L.) wheat flours	243:297	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	11	11	with	related	1416:1422	arg1	composition					1449:1459	their phytochemical composition	1429:1459	their phytochemical composition	1429:1459	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	5	12	dep	in	861:862	arg1	vitro					864:868	vitro	864:868	vitro	864:868	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	2	13	theme	turgidum	229:236	arg1	ssp					238:240	different durum (Triticum turgidum ssp	203:240	different durum (Triticum turgidum ssp	203:240	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	11	14	theme	functional	1372:1381	arg1	ingredients					1383:1393	functional ingredients	1372:1393	functional ingredients	1372:1393	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	14	theme	functional	1372:1381	arg1	benefits					1359:1366	potential health benefits	1342:1366	potential health benefits	1342:1366	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	4	15	theme	wheat	615:619	arg1	varieties					621:629	the analyzed wheat varieties	602:629	the analyzed wheat varieties	602:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	1	16	from	role	123:126	arg1	nutrition					137:145	human nutrition	131:145	human nutrition	131:145	Wheat is an important cereal with a key role in human nutrition.					
32079210	2	17	theme	Triticum	220:227	arg1	ssp					238:240	different durum (Triticum turgidum ssp	203:240	different durum (Triticum turgidum ssp	203:240	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	3	18	theme	nutritional	351:361	arg1	claims					374:379	nutritional and health claims	351:379	claims	374:379	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	11	19	theme	analyzed	1275:1282	arg1	fraction					1325:1332	bran fraction	1320:1332	bran fraction	1320:1332	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	19	theme	analyzed	1275:1282	arg1	samples					1284:1290	all analyzed samples	1271:1290	all analyzed samples	1271:1290	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	19	theme	analyzed	1275:1282	arg1	flour					1310:1314	whole flour	1304:1314	whole flour	1304:1314	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	6	20	theme	analyzed	926:933	arg1	samples					935:941	all analyzed samples	922:941	all analyzed samples	922:941	Insoluble DF was the prevailing fraction in all analyzed samples.					
32079210	4	21	theme	first	549:553	arg1	compounds					490:498	other bioactive compounds	474:498	other bioactive compounds (phenolics and tocopherols)	474:526	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	4	21	theme	first	549:553	arg1	approach					555:562	a first approach	547:562	a first approach to their phytochemical composition in the analyzed wheat varieties	547:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	0	22	theme	Health	10:15	arg1	Claims					17:22	Potential Health Claims	0:22	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.	0:81	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	3	23	theme	bread	257:261	arg1	flours					292:297	Durum L.) and bread (Triticum aestivum L.) wheat flours	243:297	Durum L.) and bread (Triticum aestivum L.) wheat flours	243:297	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	0	24	theme	Potential	0:8	arg1	Claims					17:22	Potential Health Claims	0:22	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.	0:81	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	6	25	theme	prevailing	899:908	arg1	fraction					910:917	the prevailing fraction	895:917	the prevailing fraction in all analyzed samples	895:941	Insoluble DF was the prevailing fraction in all analyzed samples.					
32079210	6	25	theme	prevailing	899:908	arg1	DF					888:889	Insoluble DF	878:889	Insoluble DF	878:889	Insoluble DF was the prevailing fraction in all analyzed samples.					
32079210	1	26	with	cereal	105:110	arg1	role					123:126	a key role	117:126	a key role in human nutrition	117:145	Wheat is an important cereal with a key role in human nutrition.					
32079210	7	27	theme	wheat	997:1001	arg1	flours					1003:1008	durum wheat flours	991:1008	durum wheat flours	991:1008	Water extractable arabinoxylans were higher in durum wheat flours.					
32079210	10	28	theme	Whole	1108:1112	arg1	flours					1114:1119	Whole flours	1108:1119	Whole flours	1108:1119	Whole flours showed higher antioxidant properties.					
32079210	5	29	theme	total	712:716	arg1	phenols					718:724	total phenols	712:724	total phenols	712:724	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	10	30	theme	higher	1128:1133	arg1	properties					1147:1156	higher antioxidant properties	1128:1156	higher antioxidant properties	1128:1156	Whole flours showed higher antioxidant properties.					
32079210	5	31	theme	colorimetric	745:756	arg1	methods					758:764	colorimetric methods	745:764	colorimetric methods	745:764	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	8	32	theme	Whole	1011:1015	arg1	flours					1017:1022	Whole flours	1011:1022	Whole flours	1011:1022	Whole flours contained higher total phenolics compounds.					
32079210	11	33	theme	approved	1223:1230	arg1	claims					1239:1244	all approved health claims	1219:1244	all approved health claims referred to wheat	1219:1262	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	1	34	theme	key	119:121	arg1	role					123:126	a key role	117:126	a key role in human nutrition	117:145	Wheat is an important cereal with a key role in human nutrition.					
32079210	11	35	theme	whole	1304:1308	arg1	samples					1284:1290	all analyzed samples	1271:1290	all analyzed samples	1271:1290	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	35	theme	whole	1304:1308	arg1	flour					1310:1314	whole flour	1304:1314	whole flour	1304:1314	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	4	36	theme	analyzed	606:613	arg1	varieties					621:629	the analyzed wheat varieties	602:629	the analyzed wheat varieties	602:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	7	37	theme	durum	991:995	arg1	flours					1003:1008	durum wheat flours	991:1008	durum wheat flours	991:1008	Water extractable arabinoxylans were higher in durum wheat flours.					
32079210	4	38	dep	compounds	490:498	arg1	tocopherols					515:525	tocopherols	515:525	tocopherols	515:525	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	4	38	dep	compounds	490:498	arg1	phenolics					501:509	phenolics	501:509	phenolics	501:509	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	11	39	theme	functional	1398:1407	arg1	foods					1409:1413	functional foods	1398:1413	functional foods	1398:1413	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	39	theme	functional	1398:1407	arg1	benefits					1359:1366	potential health benefits	1342:1366	potential health benefits	1342:1366	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	3	40	theme	health	367:372	arg1	claims					374:379	nutritional and health claims	351:379	claims	374:379	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	0	41	theme	Durum	27:31	arg1	Claims					17:22	Potential Health Claims	0:22	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.	0:81	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	6	42	theme	Insoluble	878:886	arg1	fraction					910:917	the prevailing fraction	895:917	the prevailing fraction in all analyzed samples	895:941	Insoluble DF was the prevailing fraction in all analyzed samples.					
32079210	6	42	theme	Insoluble	878:886	arg1	DF					888:889	Insoluble DF	878:889	Insoluble DF	878:889	Insoluble DF was the prevailing fraction in all analyzed samples.					
32079210	3	43	dep	Triticum	264:271	arg1	L.					282:283	Triticum aestivum L.	264:283	Triticum aestivum L.	264:283	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	3	44	dep	potential	341:349	arg1	allege					381:386	allege	381:386	allege according to the current European regulation (Regulation (EU) No 432/2012)	381:461	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	7	45	theme	Water	944:948	arg1	arabinoxylans					962:974	Water extractable arabinoxylans	944:974	Water extractable arabinoxylans	944:974	Water extractable arabinoxylans were higher in durum wheat flours.					
32079210	11	46	theme	potential	1342:1350	arg1	ingredients					1383:1393	functional ingredients	1372:1393	functional ingredients	1372:1393	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	46	theme	potential	1342:1350	arg1	foods					1409:1413	functional foods	1398:1413	functional foods	1398:1413	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	11	46	theme	potential	1342:1350	arg1	benefits					1359:1366	potential health benefits	1342:1366	potential health benefits	1342:1366	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	0	47	theme	Wheat	43:47	arg1	Flours					49:54	Bread Wheat Flours	37:54	Bread Wheat Flours	37:54	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	5	48	theme	antioxidant	804:814	arg1	activity					816:823	antioxidant activity	804:823	antioxidant activity	804:823	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	8	49	theme	phenolics	1047:1055	arg1	compounds					1057:1065	higher total phenolics compounds	1034:1065	higher total phenolics compounds	1034:1065	Whole flours contained higher total phenolics compounds.					
32079210	5	50	theme	enzymatic-gravimetric	663:683	arg1	methods					685:691	AOAC enzymatic-gravimetric methods	658:691	AOAC enzymatic-gravimetric methods	658:691	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	7	51	theme	extractable	950:960	arg1	arabinoxylans					962:974	Water extractable arabinoxylans	944:974	Water extractable arabinoxylans	944:974	Water extractable arabinoxylans were higher in durum wheat flours.					
32079210	3	52	theme	current	405:411	arg1	432/2012					453:460	Regulation (EU) No 432/2012	434:460	Regulation (EU) No 432/2012	434:460	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	3	52	theme	current	405:411	arg1	regulation					422:431	the current European regulation	401:431	the current European regulation (Regulation (EU) No 432/2012)	401:461	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	0	53	theme	Bread	37:41	arg1	Flours					49:54	Bread Wheat Flours	37:54	Bread Wheat Flours	37:54	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	8	54	theme	total	1041:1045	arg1	compounds					1057:1065	higher total phenolics compounds	1034:1065	higher total phenolics compounds	1034:1065	Whole flours contained higher total phenolics compounds.					
32079210	6	55	from	fraction	910:917	arg1	samples					935:941	all analyzed samples	922:941	all analyzed samples	922:941	Insoluble DF was the prevailing fraction in all analyzed samples.					
32079210	3	56	theme	European	413:420	arg1	432/2012					453:460	Regulation (EU) No 432/2012	434:460	Regulation (EU) No 432/2012	434:460	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	3	56	theme	European	413:420	arg1	regulation					422:431	the current European regulation	401:431	the current European regulation (Regulation (EU) No 432/2012)	401:461	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	8	57	theme	higher	1034:1039	arg1	compounds					1057:1065	higher total phenolics compounds	1034:1065	higher total phenolics compounds	1034:1065	Whole flours contained higher total phenolics compounds.					
32079210	2	58	theme	dietary	163:169	arg1	DF					178:179	DF	178:179	DF	178:179	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	2	58	theme	dietary	163:169	arg1	fiber					171:175	dietary fiber	163:175	dietary fiber (DF)	163:180	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	2	59	theme	durum	213:217	arg1	ssp					238:240	different durum (Triticum turgidum ssp	203:240	different durum (Triticum turgidum ssp	203:240	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	9	60	theme	major	1093:1097	arg1	Alpha-tocopherol					1068:1083	Alpha-tocopherol	1068:1083	Alpha-tocopherol	1068:1083	Alpha-tocopherol was the major isoform.					
32079210	9	60	theme	major	1093:1097	arg1	isoform					1099:1105	the major isoform	1089:1105	the major isoform	1089:1105	Alpha-tocopherol was the major isoform.					
32079210	5	61	theme	different	851:859	arg1	assays					870:875	three different in vitro assays	845:875	three different in vitro assays	845:875	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	11	62	theme	phytochemical	1435:1447	arg1	composition					1449:1459	their phytochemical composition	1429:1459	their phytochemical composition	1429:1459	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	3	63	theme	Durum	243:247	arg1	flours					292:297	Durum L.) and bread (Triticum aestivum L.) wheat flours	243:297	Durum L.) and bread (Triticum aestivum L.) wheat flours	243:297	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	2	64	theme	different	203:211	arg1	ssp					238:240	different durum (Triticum turgidum ssp	203:240	different durum (Triticum turgidum ssp	203:240	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	5	65	theme	in	861:862	arg1	assays					870:875	three different in vitro assays	845:875	three different in vitro assays	845:875	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	10	66	theme	antioxidant	1135:1145	arg1	properties					1147:1156	higher antioxidant properties	1128:1156	higher antioxidant properties	1128:1156	Whole flours showed higher antioxidant properties.					
32079210	5	67	theme	AOAC	658:661	arg1	methods					685:691	AOAC enzymatic-gravimetric methods	658:691	AOAC enzymatic-gravimetric methods	658:691	DF was analyzed following AOAC enzymatic-gravimetric methods; arabinoxylans and total phenols were quantified by colorimetric methods; tocopherols were determined by HPLC; antioxidant activity was evaluated using three different in vitro assays.					
32079210	3	68	theme	Regulation	434:443	arg1	432/2012					453:460	Regulation (EU) No 432/2012	434:460	Regulation (EU) No 432/2012	434:460	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	3	68	theme	Regulation	434:443	arg1	regulation					422:431	the current European regulation	401:431	the current European regulation (Regulation (EU) No 432/2012)	401:461	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	2	69	dep	fiber	171:175	arg1	study					156:160	this study	151:160	this study	151:160	In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp.					
32079210	0	70	theme	Flours	49:54	arg1	Claims					17:22	Potential Health Claims	0:22	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.	0:81	Potential Health Claims of Durum and Bread Wheat Flours as Functional Ingredients.					
32079210	3	71	theme	No	450:451	arg1	432/2012					453:460	Regulation (EU) No 432/2012	434:460	Regulation (EU) No 432/2012	434:460	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	3	71	theme	No	450:451	arg1	regulation					422:431	the current European regulation	401:431	the current European regulation (Regulation (EU) No 432/2012)	401:461	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
32079210	11	72	theme	obtained	1176:1183	arg1	results					1185:1191	the obtained results	1172:1191	the obtained results	1172:1191	According to the obtained results, it is possible to allege all approved health claims referred to wheat, since all analyzed samples, especially whole flour and bran fraction, showed potential health benefits, as functional ingredients or functional foods, related with their phytochemical composition.					
32079210	4	73	theme	phytochemical	573:585	arg1	composition					587:597	their phytochemical composition	567:597	their phytochemical composition in the analyzed wheat varieties	567:629	Moreover, other bioactive compounds (phenolics and tocopherols) were quantified as a first approach to their phytochemical composition in the analyzed wheat varieties.					
32079210	3	74	dep	Durum	243:247	arg1	L.					249:250	L.	249:250	L.	249:250	Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation (Regulation (EU) No 432/2012).					
33541624	4	0	theme	silver	692:697	arg1	nanoparticles					699:711	silver nanoparticles	692:711	silver nanoparticles	692:711	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	2	1	theme	self-growth	366:376	arg1	method					378:383	a biological blending self-growth method	344:383	a biological blending self-growth method	344:383	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	6	2	theme	wound	992:996	arg1	healing					998:1004	wound healing	992:1004	wound healing	992:1004	This allowed it to accelerate wound cell migration and promote wound healing.					
33541624	0	3	theme	antibacterial	103:115	arg1	dressing					117:124	antibacterial dressing	103:124	antibacterial dressing	103:124	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	7	4	theme	oxidizing	1101:1109	arg1	radicals					1116:1123	oxidizing free radicals	1101:1123	oxidizing free radicals that killed and bacteria	1101:1148	In addition, AgNPs immobilized on the surface released Ag+, which generated a large number of oxidizing free radicals that killed and bacteria.					
33541624	2	5	theme	blending	357:364	arg1	method					378:383	a biological blending self-growth method	344:383	a biological blending self-growth method	344:383	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	8	6	dep	it	1269:1270	arg1	prospects					1289:1297	good application prospects	1272:1297	it good application prospects for wound dressings	1269:1317	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	7	7	theme	large	1085:1089	arg1	number					1091:1096	a large number	1083:1096	a large number of oxidizing free radicals that killed and bacteria	1083:1148	In addition, AgNPs immobilized on the surface released Ag+, which generated a large number of oxidizing free radicals that killed and bacteria.					
33541624	6	8	theme	cell	965:968	arg1	migration					970:978	wound cell migration	959:978	wound cell migration	959:978	This allowed it to accelerate wound cell migration and promote wound healing.					
33541624	8	9	contain	has	1230:1232	arg2	biocompatibility					1244:1259	excellent biocompatibility	1234:1259	excellent biocompatibility	1234:1259	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	8	9	contain	has	1230:1232	arg1	film					1225:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	8	10	theme	wound	1303:1307	arg1	dressings					1309:1317	wound dressings	1303:1317	wound dressings	1303:1317	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	4	11	theme	material	735:742	arg1	surface					744:750	the material surface	731:750	the material surface	731:750	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	3	12	theme	cellulose	523:531	arg1	dressing					539:546	a novel silver-based cellulose wound dressing	502:546	a novel silver-based cellulose wound dressing	502:546	We then used polydopamine to fix AgNPs to obtain a novel silver-based cellulose wound dressing.					
33541624	8	13	theme	composite	1215:1223	arg1	film					1225:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	5	14	theme	weak	865:868	arg1	current					870:876	a weak current	863:876	a weak current	863:876	This composite film was conductive and produced a weak current, and it generated heat when a voltage was applied.					
33541624	4	15	theme	infrared	594:601	arg1	spectroscopy					603:614	infrared spectroscopy	594:614	infrared spectroscopy	594:614	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	3	16	theme	wound	533:537	arg1	dressing					539:546	a novel silver-based cellulose wound dressing	502:546	a novel silver-based cellulose wound dressing	502:546	We then used polydopamine to fix AgNPs to obtain a novel silver-based cellulose wound dressing.					
33541624	8	17	theme	in	1155:1156	arg1	tests					1177:1181	The in vitro cytotoxicity tests	1151:1181	The in vitro cytotoxicity tests	1151:1181	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	2	18	used	used	339:342	arg2	we					273:274	we	273:274	we	273:274	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	2	19	dep	oxide	291:295	arg1	GO					298:299	GO	298:299	GO	298:299	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	5	20	theme	composite	820:828	arg1	film					830:833	This composite film	815:833	This composite film	815:833	This composite film was conductive and produced a weak current, and it generated heat when a voltage was applied.					
33541624	4	21	theme	composite	554:562	arg1	material					564:571	This composite material	549:571	This composite material	549:571	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	8	22	theme	rGO	1210:1212	arg1	film					1225:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	2	23	theme	culture	320:326	arg1	medium					328:333	an acetobacter culture medium	305:333	an acetobacter culture medium	305:333	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	2	24	theme	mixed-growth	434:445	arg1	film					447:450	a mixed-growth film	432:450	a mixed-growth film	432:450	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	6	25	theme	wound	959:963	arg1	migration					970:978	wound cell migration	959:978	wound cell migration	959:978	This allowed it to accelerate wound cell migration and promote wound healing.					
33541624	2	26	theme	acetobacter	308:318	arg1	medium					328:333	an acetobacter culture medium	305:333	an acetobacter culture medium	305:333	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	1	27	theme	wound	233:237	arg1	dressings					239:247	wound dressings	233:247	wound dressings	233:247	It is difficult to obtain stable multifunctional silver-containing materials that are suitable for use as wound dressings.					
33541624	8	28	theme	cytotoxicity	1164:1175	arg1	tests					1177:1181	The in vitro cytotoxicity tests	1151:1181	The in vitro cytotoxicity tests	1151:1181	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	3	29	used	used	461:464	arg2	We					453:454	We	453:454	We	453:454	We then used polydopamine to fix AgNPs to obtain a novel silver-based cellulose wound dressing.					
33541624	3	30	theme	novel	504:508	arg1	dressing					539:546	a novel silver-based cellulose wound dressing	502:546	a novel silver-based cellulose wound dressing	502:546	We then used polydopamine to fix AgNPs to obtain a novel silver-based cellulose wound dressing.					
33541624	0	31	theme	bacterial	41:49	arg1	oxide-silver					76:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	7	32	theme	free	1111:1114	arg1	radicals					1116:1123	oxidizing free radicals	1101:1123	oxidizing free radicals that killed and bacteria	1101:1148	In addition, AgNPs immobilized on the surface released Ag+, which generated a large number of oxidizing free radicals that killed and bacteria.					
33541624	0	33	theme	conductive	30:39	arg1	oxide-silver					76:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	8	34	theme	application	1277:1287	arg1	prospects					1289:1297	good application prospects	1272:1297	it good application prospects for wound dressings	1269:1317	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	2	35	theme	bacterial	404:412	arg1	cellulose					414:422	the bacterial cellulose	400:422	the bacterial cellulose	400:422	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	0	36	theme	graphene	67:74	arg1	oxide-silver					76:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	2	37	theme	biological	346:355	arg1	method					378:383	a biological blending self-growth method	344:383	a biological blending self-growth method	344:383	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	0	38	theme	cellulose-based	51:65	arg1	oxide-silver					76:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	conductive bacterial cellulose-based graphene oxide-silver	30:87	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	7	39	theme	radicals	1116:1123	arg1	number					1091:1096	a large number	1083:1096	a large number of oxidizing free radicals that killed and bacteria	1083:1148	In addition, AgNPs immobilized on the surface released Ag+, which generated a large number of oxidizing free radicals that killed and bacteria.					
33541624	4	40	theme	bacterial	794:802	arg1	cellulose					804:812	bacterial cellulose	794:812	bacterial cellulose	794:812	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	1	41	theme	stable	153:158	arg1	materials					194:202	stable multifunctional silver-containing materials	153:202	stable multifunctional silver-containing materials that are suitable for use as wound dressings	153:247	It is difficult to obtain stable multifunctional silver-containing materials that are suitable for use as wound dressings.					
33541624	4	42	theme	electron	617:624	arg1	microscopy					626:635	electron microscopy	617:635	electron microscopy	617:635	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	8	43	dep	in	1155:1156	arg1	vitro					1158:1162	vitro	1158:1162	vitro	1158:1162	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	2	44	theme	graphene	282:289	arg1	oxide					291:295	graphene oxide	282:295	graphene oxide (GO)	282:300	To solve this problem, we added graphene oxide (GO) to an acetobacter culture medium and used a biological blending self-growth method to fix GO onto the bacterial cellulose to form a mixed-growth film.					
33541624	1	45	theme	multifunctional	160:174	arg1	materials					194:202	stable multifunctional silver-containing materials	153:202	stable multifunctional silver-containing materials that are suitable for use as wound dressings	153:247	It is difficult to obtain stable multifunctional silver-containing materials that are suitable for use as wound dressings.					
33541624	8	46	theme	Ag-pDA/BC	1199:1207	arg1	film					1225:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	the Ag-pDA/BC (rGO) composite film	1195:1228	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	8	47	theme	good	1272:1275	arg1	prospects					1289:1297	good application prospects	1272:1297	it good application prospects for wound dressings	1269:1317	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	8	48	theme	excellent	1234:1242	arg1	biocompatibility					1244:1259	excellent biocompatibility	1234:1259	excellent biocompatibility	1234:1259	The in vitro cytotoxicity tests showed that the Ag-pDA/BC (rGO) composite film has excellent biocompatibility, giving it good application prospects for wound dressings.					
33541624	4	49	theme	X-ray	642:646	arg1	diffractometry					648:661	X-ray diffractometry	642:661	X-ray diffractometry	642:661	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	1	50	theme	silver-containing	176:192	arg1	materials					194:202	stable multifunctional silver-containing materials	153:202	stable multifunctional silver-containing materials that are suitable for use as wound dressings	153:247	It is difficult to obtain stable multifunctional silver-containing materials that are suitable for use as wound dressings.					
33541624	0	51	theme	oxide-silver	76:87	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	0	51	theme	oxide-silver	76:87	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of conductive bacterial cellulose-based graphene oxide-silver nanoparticles antibacterial dressing.					
33541624	4	52	theme	cellulose	804:812	arg1	layer					785:789	a layer	783:789	a layer of bacterial cellulose	783:812	This composite material was characterized by infrared spectroscopy, electron microscopy, and X-ray diffractometry, and the results showed that silver nanoparticles uniformly covered the material surface, while graphene was wrapped in a layer of bacterial cellulose.					
33541624	3	53	theme	silver-based	510:521	arg1	dressing					539:546	a novel silver-based cellulose wound dressing	502:546	a novel silver-based cellulose wound dressing	502:546	We then used polydopamine to fix AgNPs to obtain a novel silver-based cellulose wound dressing.					
32564833	2	0	theme	physiological	525:537	arg1	environment					539:549	physiological environment	525:549	physiological environment	525:549	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	4	1	theme	simulated	792:800	arg1	fluid					807:811	simulated body fluid	792:811	simulated body fluid (SBF)	792:817	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	4	1	theme	simulated	792:800	arg1	SBF					814:816	SBF	814:816	SBF	814:816	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	7	2	theme	improved	1131:1138	arg1	biocompatibility					1140:1155	the improved biocompatibility	1127:1155	the improved biocompatibility of the composite coatings	1127:1181	In-vitro cell culture studies validated the improved biocompatibility of the composite coatings.					
32564833	3	3	theme	Various	552:558	arg1	techniques					600:609	Various surface and structural characterization techniques	552:609	Various surface and structural characterization techniques	552:609	Various surface and structural characterization techniques were deployed to examine the composite coatings.					
32564833	5	4	theme	drug	824:827	arg1	kinetics					837:844	The drug release kinetics	820:844	The drug release kinetics	820:844	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	9	5	theme	lowest	1274:1279	arg1	rate					1291:1294	the lowest corrosion rate	1270:1294	the lowest corrosion rate	1270:1294	% of GM exhibited the lowest corrosion rate, enhanced biocompatibility, and antibacterial performance.					
32564833	3	6	theme	surface	560:566	arg1	techniques					600:609	Various surface and structural characterization techniques	552:609	Various surface and structural characterization techniques	552:609	Various surface and structural characterization techniques were deployed to examine the composite coatings.					
32564833	6	7	theme	composite	985:993	arg1	coatings					995:1002	The pectin composite coatings	974:1002	The pectin composite coatings	974:1002	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	9	8	theme	corrosion	1281:1289	arg1	rate					1291:1294	the lowest corrosion rate	1270:1294	the lowest corrosion rate	1270:1294	% of GM exhibited the lowest corrosion rate, enhanced biocompatibility, and antibacterial performance.					
32564833	2	9	theme	multifunctional	298:312	arg1	coatings					352:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings	292:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM)	292:387	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	4	10	theme	vitro	663:667	arg1	analysis					679:686	in vitro corrosion analysis	660:686	in vitro corrosion analysis	660:686	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	0	11	theme	orthopaedic	106:116	arg1	applications					118:129	orthopaedic applications	106:129	orthopaedic applications	106:129	Preparation and characterization of Pectin/Polypyrrole based multifunctional coatings on TiNbZr alloy for orthopaedic applications.					
32564833	6	12	theme	pectin	978:983	arg1	coatings					995:1002	The pectin composite coatings	974:1002	The pectin composite coatings	974:1002	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	2	13	theme	novel	292:296	arg1	coatings					352:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings	292:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM)	292:387	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	1	14	theme	renewable	152:160	arg1	polysaccharide					162:175	a natural and renewable polysaccharide	138:175	a natural and renewable polysaccharide	138:175	Being a natural and renewable polysaccharide, pectin (PC) is considered a polymer with promising potential for many applications.					
32564833	3	15	theme	characterization	583:598	arg1	techniques					600:609	Various surface and structural characterization techniques	552:609	Various surface and structural characterization techniques	552:609	Various surface and structural characterization techniques were deployed to examine the composite coatings.					
32564833	4	16	theme	in	660:661	arg1	analysis					679:686	in vitro corrosion analysis	660:686	in vitro corrosion analysis	660:686	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	9	17	theme	enhanced	1297:1304	arg1	biocompatibility					1306:1321	enhanced biocompatibility	1297:1321	enhanced biocompatibility	1297:1321	% of GM exhibited the lowest corrosion rate, enhanced biocompatibility, and antibacterial performance.					
32564833	7	18	theme	In-vitro	1087:1094	arg1	studies					1109:1115	In-vitro cell culture studies	1087:1115	In-vitro cell culture studies	1087:1115	In-vitro cell culture studies validated the improved biocompatibility of the composite coatings.					
32564833	2	19	theme	antibacterial	471:483	arg1	performance					485:495	antibacterial performance	471:495	antibacterial performance	471:495	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	5	20	theme	release	829:835	arg1	kinetics					837:844	The drug release kinetics	820:844	The drug release kinetics	820:844	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	4	21	theme	corrosion	757:765	arg1	performance					777:787	higher corrosion resistant performance	750:787	higher corrosion resistant performance	750:787	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	5	22	theme	sustained	893:901	arg1	release					903:909	the sustained release	889:909	the sustained release of GM from the controlled degradation of the composite matrix	889:971	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	3	23	theme	composite	640:648	arg1	coatings					650:657	the composite coatings	636:657	the composite coatings	636:657	Various surface and structural characterization techniques were deployed to examine the composite coatings.					
32564833	4	24	theme	higher	750:755	arg1	performance					777:787	higher corrosion resistant performance	750:787	higher corrosion resistant performance	750:787	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	8	25	theme	developed	1194:1202	arg1	coatings					1204:1211	the developed coatings	1190:1211	the developed coatings	1190:1211	Among the developed coatings, composite coatings loaded with 10 wt.					
32564833	6	26	theme	sustained	1062:1070	arg1	release					1072:1078	the sustained release	1058:1078	the sustained release of GM	1058:1084	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	7	27	theme	coatings	1174:1181	arg1	biocompatibility					1140:1155	the improved biocompatibility	1127:1155	the improved biocompatibility of the composite coatings	1127:1181	In-vitro cell culture studies validated the improved biocompatibility of the composite coatings.					
32564833	4	28	theme	composite	707:715	arg1	specimen					731:738	the composite coated TiNbZr specimen	703:738	the composite coated TiNbZr specimen	703:738	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	2	29	theme	PC/PPy	334:339	arg1	coatings					352:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings	292:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM)	292:387	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	9	30	theme	antibacterial	1328:1340	arg1	performance					1342:1352	antibacterial performance	1328:1352	antibacterial performance	1328:1352	% of GM exhibited the lowest corrosion rate, enhanced biocompatibility, and antibacterial performance.					
32564833	2	31	theme	pectin/polypyrrole	314:331	arg1	coatings					352:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings	292:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM)	292:387	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	4	32	theme	body	802:805	arg1	fluid					807:811	simulated body fluid	792:811	simulated body fluid (SBF)	792:817	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	4	32	theme	body	802:805	arg1	SBF					814:816	SBF	814:816	SBF	814:816	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	4	33	theme	coated	717:722	arg1	specimen					731:738	the composite coated TiNbZr specimen	703:738	the composite coated TiNbZr specimen	703:738	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	5	34	theme	composite	956:964	arg1	matrix					966:971	the composite matrix	952:971	the composite matrix	952:971	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	4	35	theme	TiNbZr	724:729	arg1	specimen					731:738	the composite coated TiNbZr specimen	703:738	the composite coated TiNbZr specimen	703:738	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	2	36	theme	corrosion	501:509	arg1	resistance					511:520	corrosion resistance	501:520	corrosion resistance	501:520	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	6	37	theme	due	1051:1053	arg1	performance					1038:1048	effective antibacterial performance	1014:1048	effective antibacterial performance; due to the sustained release of GM	1014:1084	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	0	38	theme	Pectin/Polypyrrole	36:53	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of Pectin/Polypyrrole based multifunctional coatings on TiNbZr alloy for orthopaedic applications.					
32564833	0	38	theme	Pectin/Polypyrrole	36:53	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of Pectin/Polypyrrole based multifunctional coatings on TiNbZr alloy for orthopaedic applications.					
32564833	5	39	theme	controlled	926:935	arg1	degradation					937:947	the controlled degradation	922:947	the controlled degradation of the composite matrix	922:971	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	2	40	theme	TiNbZr	425:430	arg1	alloy					432:436	TiNbZr alloy	425:436	TiNbZr alloy	425:436	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	8	41	theme	composite	1214:1222	arg1	coatings					1224:1231	composite coatings	1214:1231	composite coatings	1214:1231	Among the developed coatings, composite coatings loaded with 10 wt.					
32564833	7	42	theme	composite	1164:1172	arg1	coatings					1174:1181	the composite coatings	1160:1181	the composite coatings	1160:1181	In-vitro cell culture studies validated the improved biocompatibility of the composite coatings.					
32564833	0	43	theme	multifunctional	61:75	arg1	coatings					77:84	multifunctional coatings	61:84	multifunctional coatings	61:84	Preparation and characterization of Pectin/Polypyrrole based multifunctional coatings on TiNbZr alloy for orthopaedic applications.					
32564833	7	44	theme	cell	1096:1099	arg1	studies					1109:1115	In-vitro cell culture studies	1087:1115	In-vitro cell culture studies	1087:1115	In-vitro cell culture studies validated the improved biocompatibility of the composite coatings.					
32564833	1	45	theme	natural	140:146	arg1	polysaccharide					162:175	a natural and renewable polysaccharide	138:175	a natural and renewable polysaccharide	138:175	Being a natural and renewable polysaccharide, pectin (PC) is considered a polymer with promising potential for many applications.					
32564833	6	46	theme	antibacterial	1024:1036	arg1	performance					1038:1048	effective antibacterial performance	1014:1048	effective antibacterial performance; due to the sustained release of GM	1014:1084	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	2	47	theme	present	269:275	arg1	investigation					277:289	the present investigation	265:289	the present investigation	265:289	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	4	48	theme	resistant	767:775	arg1	performance					777:787	higher corrosion resistant performance	750:787	higher corrosion resistant performance	750:787	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	7	49	theme	culture	1101:1107	arg1	studies					1109:1115	In-vitro cell culture studies	1087:1115	In-vitro cell culture studies	1087:1115	In-vitro cell culture studies validated the improved biocompatibility of the composite coatings.					
32564833	9	50	theme	GM	1257:1258	arg1	GM					1257:1258	GM	1257:1258	GM	1257:1258	% of GM exhibited the lowest corrosion rate, enhanced biocompatibility, and antibacterial performance.					
32564833	9	50	theme	GM	1257:1258	arg1	%					1252:1252	%	1252:1252	% of GM	1252:1258	% of GM exhibited the lowest corrosion rate, enhanced biocompatibility, and antibacterial performance.					
32564833	2	51	theme	composite	342:350	arg1	coatings					352:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings	292:359	novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM)	292:387	In the present investigation, novel multifunctional pectin/polypyrrole (PC/PPy) composite coatings loaded with gentamicin (GM) were electrochemically deposited on TiNbZr alloy to enhance its biocompatibility, antibacterial performance and corrosion resistance in physiological environment.					
32564833	4	52	theme	corrosion	669:677	arg1	analysis					679:686	in vitro corrosion analysis	660:686	in vitro corrosion analysis	660:686	in vitro corrosion analysis confirmed that the composite coated TiNbZr specimen exhibited higher corrosion resistant performance in simulated body fluid (SBF).					
32564833	0	53	theme	TiNbZr	89:94	arg1	alloy					96:100	TiNbZr alloy	89:100	TiNbZr alloy for orthopaedic applications	89:129	Preparation and characterization of Pectin/Polypyrrole based multifunctional coatings on TiNbZr alloy for orthopaedic applications.					
32564833	1	54	theme	promising	219:227	arg1	potential					229:237	promising potential	219:237	promising potential for many applications	219:259	Being a natural and renewable polysaccharide, pectin (PC) is considered a polymer with promising potential for many applications.					
32564833	5	55	theme	GM	914:915	arg1	release					903:909	the sustained release	889:909	the sustained release of GM from the controlled degradation of the composite matrix	889:971	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	5	56	theme	matrix	966:971	arg1	degradation					937:947	the controlled degradation	922:947	the controlled degradation of the composite matrix	922:971	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	1	57	theme	many	243:246	arg1	applications					248:259	many applications	243:259	many applications	243:259	Being a natural and renewable polysaccharide, pectin (PC) is considered a polymer with promising potential for many applications.					
32564833	6	58	theme	GM	1083:1084	arg1	release					1072:1078	the sustained release	1058:1078	the sustained release of GM	1058:1084	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	6	59	theme	effective	1014:1022	arg1	performance					1038:1048	effective antibacterial performance	1014:1048	effective antibacterial performance; due to the sustained release of GM	1014:1084	The pectin composite coatings exhibited effective antibacterial performance; due to the sustained release of GM.					
32564833	5	60	from	degradation	937:947	arg1	release					903:909	the sustained release	889:909	the sustained release of GM from the controlled degradation of the composite matrix	889:971	The drug release kinetics was estimated and the results corroborated the sustained release of GM from the controlled degradation of the composite matrix.					
32564833	3	61	theme	structural	572:581	arg1	characterization					583:598	structural characterization	572:598	structural characterization	572:598	Various surface and structural characterization techniques were deployed to examine the composite coatings.					
32564833	1	62	with	polymer	206:212	arg1	potential					229:237	promising potential	219:237	promising potential for many applications	219:259	Being a natural and renewable polysaccharide, pectin (PC) is considered a polymer with promising potential for many applications.					
32311292	0	0	theme	Milk	70:73	arg1	Processes					83:91	Milk Banking Processes	70:91	Milk Banking Processes	70:91	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	0	1	from	Protein	8:14	arg1	Associations					52:63	Associations	52:63	Associations with Milk Banking Processes	52:91	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	0	1	from	Protein	8:14	arg1	Milk					43:46	Donor Human Milk	31:46	Donor Human Milk	31:46	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	1	2	theme	macronutrient	134:146	arg1	variability					148:158	macronutrient variability	134:158	macronutrient variability in donor human milk (DHM)	134:184	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	2	3	from	composition	256:266	arg1	DHM					271:273	DHM	271:273	DHM	271:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	6	4	dep	1.4 g/dL	872:879	arg1	to					869:870	to	869:870	to	869:870	: Fat ranged from 1.9 to 6.1 g/dL (n = 298) and protein ranged from 0.7 to 1.4 g/dL (n = 300).					
32311292	3	5	dep	along	418:422	arg1	with					424:427	with	424:427	with	424:427	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	7	6	theme	more	996:999	arg1	donors					1001:1006	more donors	996:1006	more donors	996:1006	Variability in fat was significantly lower in samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001).					
32311292	2	7	theme	modifiable	315:324	arg1	processes					336:344	modifiable milk bank processes	315:344	modifiable milk bank processes	315:344	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	0	8	from	Fat	0:2	arg1	Associations					52:63	Associations	52:63	Associations with Milk Banking Processes	52:91	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	0	8	from	Fat	0:2	arg1	Milk					43:46	Donor Human Milk	31:46	Donor Human Milk	31:46	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	1	9	from	variability	148:158	arg1	DHM					181:183	DHM	181:183	DHM	181:183	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	1	9	from	variability	148:158	arg1	milk					175:178	donor human milk	163:178	donor human milk (DHM)	163:184	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	2	10	theme	protein	248:254	arg1	composition					256:266	protein composition	248:266	protein composition	248:266	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	2	11	from	DHM	271:273	arg1	understanding					223:235	a better understanding	214:235	a better understanding of fat and protein composition in DHM	214:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	0	12	theme	Banking	75:81	arg1	Processes					83:91	Milk Banking Processes	70:91	Milk Banking Processes	70:91	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	0	13	with	Associations	52:63	arg1	Processes					83:91	Milk Banking Processes	70:91	Milk Banking Processes	70:91	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	2	14	theme	milk	326:329	arg1	processes					336:344	modifiable milk bank processes	315:344	modifiable milk bank processes	315:344	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	6	15	dep	6.1 g/dL	822:829	arg1	to					819:820	to	819:820	to	819:820	: Fat ranged from 1.9 to 6.1 g/dL (n = 298) and protein ranged from 0.7 to 1.4 g/dL (n = 300).					
32311292	10	16	theme	greater	1371:1377	arg1	number					1379:1384	a greater number	1369:1384	a greater number of donors in a pool	1369:1404	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	2	17	theme	fat	240:242	arg1	understanding					223:235	a better understanding	214:235	a better understanding of fat and protein composition in DHM	214:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	3	18	used	used	496:499	arg2	analysis					483:490	macronutrient analysis	469:490	macronutrient analysis	469:490	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	19	dep	pooling	522:528	arg1	pooling					538:544	target pooling	531:544	target pooling; yes/no	531:552	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	20	theme	pooled	363:368	arg1	raw					358:360	raw	358:360	raw	358:360	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	20	theme	pooled	363:368	arg1	DHM					370:372	pooled DHM	363:372	pooled DHM	363:372	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	1	21	theme	donor	163:167	arg1	DHM					181:183	DHM	181:183	DHM	181:183	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	1	21	theme	donor	163:167	arg1	milk					175:178	donor human milk	163:178	donor human milk (DHM)	163:184	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	1	22	theme	human	169:173	arg1	DHM					181:183	DHM	181:183	DHM	181:183	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	1	22	theme	human	169:173	arg1	milk					175:178	donor human milk	163:178	donor human milk (DHM)	163:184	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	3	23	theme	raw	358:360	arg1	Samples					347:353	Samples	347:353	Samples of raw, pooled DHM	347:372	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	10	24	from	number	1379:1384	arg1	pool					1401:1404	a pool	1399:1404	a pool	1399:1404	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	1	25	from	impact	98:103	arg1	variability					148:158	macronutrient variability	134:158	macronutrient variability in donor human milk (DHM)	134:184	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	3	26	dep	pooling	538:544	arg1	yes/no					547:552	yes/no	547:552	target pooling; yes/no	531:552	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	10	27	theme	milk	1327:1330	arg1	banks					1332:1336	milk banks	1327:1336	milk banks that do not target pool	1327:1360	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	9	28	theme	nutrient	1262:1269	arg1	variability					1271:1281	nutrient variability	1262:1281	nutrient variability	1262:1281	In a stratified analysis, increasing the number of donors per pool only reduced nutrient variability in samples that were not target pooled.					
32311292	10	29	theme	donors	1389:1394	arg1	number					1379:1384	a greater number	1369:1384	a greater number of donors in a pool	1369:1404	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	3	30	theme	macronutrient	469:481	arg1	analysis					483:490	macronutrient analysis	469:490	macronutrient analysis	469:490	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	31	theme	donors	566:571	arg1	glass/plastic/other					611:629	glass/plastic/other	611:629	glass/plastic/other	611:629	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	31	theme	donors	566:571	arg1	pooling					522:528	pooling	522:528	pooling (target pooling; yes/no)	522:553	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	31	theme	donors	566:571	arg1	method					637:642	method	637:642	method for mixing during bottling (manual/mechanical)	637:689	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	31	theme	donors	566:571	arg1	number					556:561	number	556:561	number of donors per pool	556:580	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	31	theme	donors	566:571	arg1	material					601:608	pooling container material	583:608	pooling container material (glass/plastic/other)	583:630	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	2	32	theme	composition	256:266	arg1	understanding					223:235	a better understanding	214:235	a better understanding of fat and protein composition in DHM	214:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	2	33	from	understanding	223:235	arg1	DHM					271:273	DHM	271:273	DHM	271:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	7	34	from	Variability	892:902	arg1	fat					907:909	fat	907:909	fat	907:909	Variability in fat was significantly lower in samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001).					
32311292	0	35	theme	Donor	31:35	arg1	Milk					43:46	Donor Human Milk	31:46	Donor Human Milk	31:46	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	3	36	theme	target	531:536	arg1	pooling					538:544	target pooling	531:544	target pooling; yes/no	531:552	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	10	37	theme	protein	1425:1431	arg1	variability					1433:1443	fat and protein variability	1417:1443	fat and protein variability	1417:1443	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	5	38	theme	spread	774:779	arg1	magnitude					757:765	magnitude	757:765	magnitude of the spread	757:779	Homoscedasticity was assessed and magnitude of the spread was quantified.					
32311292	9	39	theme	stratified	1187:1196	arg1	analysis					1198:1205	a stratified analysis	1185:1205	a stratified analysis	1185:1205	In a stratified analysis, increasing the number of donors per pool only reduced nutrient variability in samples that were not target pooled.					
32311292	7	40	dep	pooled	967:972	arg1	p = 0.04					975:982	p = 0.04	975:982	p = 0.04	975:982	Variability in fat was significantly lower in samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001).					
32311292	8	41	theme	more	1148:1151	arg1	donors					1153:1158	more donors	1148:1158	more donors	1148:1158	Variability in protein was significantly lower in samples that contained more donors per pool (p = 0.001).					
32311292	10	42	from	donors	1389:1394	arg1	pool					1401:1404	a pool	1399:1404	a pool	1399:1404	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	3	43	theme	processing	443:452	arg1	attributes					454:463	the following processing attributes	429:463	20 milk banks (n = 300) along with the following processing attributes	394:463	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	2	44	with	relationships	296:308	arg1	processes					336:344	modifiable milk bank processes	315:344	modifiable milk bank processes	315:344	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	0	45	theme	Human	37:41	arg1	Milk					43:46	Donor Human Milk	31:46	Donor Human Milk	31:46	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	3	46	theme	pooling	583:589	arg1	glass/plastic/other					611:629	glass/plastic/other	611:629	glass/plastic/other	611:629	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	46	theme	pooling	583:589	arg1	material					601:608	pooling container material	583:608	pooling container material (glass/plastic/other)	583:630	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	47	theme	following	433:441	arg1	attributes					454:463	the following processing attributes	429:463	20 milk banks (n = 300) along with the following processing attributes	394:463	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	2	48	from	fat	240:242	arg1	DHM					271:273	DHM	271:273	DHM	271:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	7	49	dep	mixed	1043:1047	arg1	p < 0.001					1063:1071	p < 0.001	1063:1071	p < 0.001	1063:1071	Variability in fat was significantly lower in samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001).					
32311292	3	50	theme	container	591:599	arg1	glass/plastic/other					611:629	glass/plastic/other	611:629	glass/plastic/other	611:629	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	50	theme	container	591:599	arg1	material					601:608	pooling container material	583:608	pooling container material (glass/plastic/other)	583:630	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	8	51	from	Variability	1075:1085	arg1	protein					1090:1096	protein	1090:1096	protein	1090:1096	Variability in protein was significantly lower in samples that contained more donors per pool (p = 0.001).					
32311292	1	52	theme	milk	108:111	arg1	processes					121:129	milk banking processes	108:129	milk banking processes	108:129	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	8	53	contain	contained	1138:1146	arg2	donors					1153:1158	more donors	1148:1158	more donors	1148:1158	Variability in protein was significantly lower in samples that contained more donors per pool (p = 0.001).					
32311292	8	53	contain	contained	1138:1146	arg1	samples					1125:1131	samples	1125:1131	samples that contained more donors per pool (p = 0.001)	1125:1179	Variability in protein was significantly lower in samples that contained more donors per pool (p = 0.001).					
32311292	2	54	theme	better	216:221	arg1	understanding					223:235	a better understanding	214:235	a better understanding of fat and protein composition in DHM	214:273	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	0	55	with	Milk	43:46	arg1	Processes					83:91	Milk Banking Processes	70:91	Milk Banking Processes	70:91	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	1	56	theme	banking	113:119	arg1	processes					121:129	milk banking processes	108:129	milk banking processes	108:129	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	2	57	theme	potential	286:294	arg1	relationships					296:308	potential relationships	286:308	potential relationships with modifiable milk bank processes	286:344	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	10	58	from	pool	1401:1404	arg1	number					1379:1384	a greater number	1369:1384	a greater number of donors in a pool	1369:1404	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	7	59	contain	contained	986:994	arg2	donors					1001:1006	more donors	996:1006	more donors	996:1006	Variability in fat was significantly lower in samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001).					
32311292	7	59	contain	contained	986:994	arg1	samples					938:944	samples	938:944	samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001)	938:1072	Variability in fat was significantly lower in samples that had been target pooled (p = 0.04), contained more donors per pool (p < 0.001), and had been mixed mechanically (p < 0.001).					
32311292	1	60	theme	processes	121:129	arg1	impact					98:103	The impact	94:103	The impact of milk banking processes on macronutrient variability in donor human milk (DHM)	94:184	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	1	60	theme	processes	121:129	arg1	unknown					197:203	unknown	197:203	unknown	197:203	The impact of milk banking processes on macronutrient variability in donor human milk (DHM) is largely unknown.					
32311292	3	61	theme	milk	397:400	arg1	n = 300					409:415	n = 300	409:415	n = 300	409:415	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	3	61	theme	milk	397:400	arg1	banks					402:406	20 milk banks	394:406	20 milk banks (n = 300) along with the following processing attributes	394:463	Samples of raw, pooled DHM were collected from 20 milk banks (n = 300) along with the following processing attributes: if macronutrient analysis was used to select donors for pooling (target pooling; yes/no), number of donors per pool, pooling container material (glass/plastic/other), and method for mixing during bottling (manual/mechanical).					
32311292	0	62	dep	Fat	0:2	arg1	Variability					16:26	Variability	16:26	Variability	16:26	Fat and Protein Variability in Donor Human Milk and Associations with Milk Banking Processes.					
32311292	2	63	theme	bank	331:334	arg1	processes					336:344	modifiable milk bank processes	315:344	modifiable milk bank processes	315:344	To gain a better understanding of fat and protein composition in DHM and assess potential relationships with modifiable milk bank processes.					
32311292	10	64	theme	fat	1417:1419	arg1	variability					1433:1443	fat and protein variability	1417:1443	fat and protein variability	1417:1443	For milk banks that do not target pool, using a greater number of donors in a pool may reduce fat and protein variability.					
32311292	9	65	theme	donors	1233:1238	arg1	number					1223:1228	the number	1219:1228	the number of donors per pool	1219:1247	In a stratified analysis, increasing the number of donors per pool only reduced nutrient variability in samples that were not target pooled.					
32228300	8	0	theme	12th	1054:1057	arg1	week					1059:1062	the 12th week	1050:1062	the 12th week	1050:1062	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	5	1	theme	MC	658:659	arg1	group					662:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group	575:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet)	575:676	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	5	1	theme	MC	658:659	arg1	MC diet					669:675	MC diet	669:675	MC diet	669:675	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	13	2	theme	GPR43	1838:1842	arg1	down-regulation					1819:1833	down-regulation	1819:1833	down-regulation of GPR43	1819:1842	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	5	3	theme	and maternal	636:647	arg1	group					662:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group	575:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet)	575:676	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	5	3	theme	and maternal	636:647	arg1	MC diet					669:675	MC diet	669:675	MC diet	669:675	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	9	4	theme	intestinal	1100:1109	arg1	development					1111:1121	intestinal development	1100:1121	intestinal development	1100:1121	RESULTS MS significantly inhibited intestinal development and disrupted barrier function in 3-week-old offspring.					
32228300	10	5	theme	GPR43	1402:1406	arg1	down-regulation					1383:1397	down-regulation	1383:1397	down-regulation of GPR43	1383:1406	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	9	6	theme	barrier	1137:1143	arg1	function					1145:1152	disrupted barrier function	1127:1152	disrupted barrier function	1127:1152	RESULTS MS significantly inhibited intestinal development and disrupted barrier function in 3-week-old offspring.					
32228300	13	7	theme	MS	1733:1734	arg1	intake					1736:1741	CONCLUSIONS MS intake	1721:1741	CONCLUSIONS MS intake	1721:1741	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	2	8	theme	Excessive	221:229	arg1	sucralose					231:239	Excessive sucralose	221:239	Excessive sucralose	221:239	Excessive sucralose can induce gut dysbiosis and negatively affect host health.					
32228300	3	9	theme	Maternal	301:308	arg1	diet					310:313	Maternal diet	301:313	Maternal diet	301:313	Maternal diet shapes the microbial communities of neonate and this effect continues in later life.					
32228300	4	10	theme	sucralose 	448:457	arg1	intake					463:468	maternal sucralose (MS) intake	439:468	maternal sucralose (MS) intake	439:468	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	5	11	theme	METHODS	540:546	arg1	mice					565:568	METHODS C57BL/6 pregnant mice	540:568	METHODS C57BL/6 pregnant mice	540:568	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	1	12	theme	fatty	133:137	arg1	NAFLD					154:158	NAFLD	154:158	NAFLD	154:158	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	1	12	theme	fatty	133:137	arg1	disease					145:151	BACKGROUND Nonalcoholic fatty liver disease	109:151	BACKGROUND Nonalcoholic fatty liver disease (NAFLD)	109:159	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	12	13	theme	MC	1711:1712	arg1	group					1714:1718	MC group	1711:1718	MC group	1711:1718	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	10	14	dep	compositions	1256:1267	arg1	the					1252:1254	the	1252:1254	the	1252:1254	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	2	15	theme	gut	252:254	arg1	dysbiosis					256:264	gut dysbiosis	252:264	gut dysbiosis	252:264	Excessive sucralose can induce gut dysbiosis and negatively affect host health.					
32228300	7	16	theme	3-week-old	905:914	arg1	offspring					916:924	the 3-week-old offspring	901:924	the 3-week-old offspring	901:924	The intestinal development, mucosal barrier function, and gut microbiota were assessed in the 3-week-old offspring.					
32228300	3	17	theme	microbial	326:334	arg1	communities					336:346	the microbial communities	322:346	the microbial communities of neonate	322:357	Maternal diet shapes the microbial communities of neonate and this effect continues in later life.					
32228300	12	18	theme	gut	1683:1685	arg1	dysbiosis					1687:1695	gut dysbiosis	1683:1695	gut dysbiosis	1683:1695	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	12	19	theme	HFD	1556:1558	arg1	treatment					1560:1568	HFD treatment	1556:1568	HFD treatment	1556:1568	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	0	20	theme	hepatic	77:83	arg1	steatosis					85:93	hepatic steatosis	77:93	hepatic steatosis in adulthood	77:106	Maternal sucralose intake alters gut microbiota of offspring and exacerbates hepatic steatosis in adulthood.					
32228300	5	21	theme	into MS	586:592	arg1	group					594:598	randomized into MS group	575:598	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet)	575:676	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	11	22	theme	Clostridium	1518:1528	arg1	one					1478:1480	one	1478:1480	one	1478:1480	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	11	22	theme	Clostridium	1518:1528	arg1	bacteria					1508:1515	the butyrate-producing bacteria	1485:1515	the butyrate-producing bacteria	1485:1515	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	11	22	theme	Clostridium	1518:1528	arg1	butyricum					1530:1538	Clostridium butyricum	1518:1538	Clostridium butyricum	1518:1538	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	11	23	theme	butyrate-producing	1489:1506	arg1	bacteria					1508:1515	the butyrate-producing bacteria	1485:1515	the butyrate-producing bacteria	1485:1515	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	8	24	theme	hepatic	953:959	arg1	steatosis					961:969	hepatic steatosis	953:969	hepatic steatosis	953:969	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	7	25	theme	gut	869:871	arg1	microbiota					873:882	gut microbiota	869:882	gut microbiota	869:882	The intestinal development, mucosal barrier function, and gut microbiota were assessed in the 3-week-old offspring.					
32228300	11	26	theme	blocking	1423:1430	arg1	GPR43					1432:1436	blocking GPR43	1423:1436	blocking GPR43	1423:1436	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	5	27	theme	pregnant	556:563	arg1	mice					565:568	METHODS C57BL/6 pregnant mice	540:568	METHODS C57BL/6 pregnant mice	540:568	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	4	28	theme	intake	463:468	arg1	effects					428:434	the effects	424:434	the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood	424:537	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	10	29	theme	low-grade	1206:1214	arg1	inflammation					1216:1227	intestinal low-grade inflammation	1195:1227	intestinal low-grade inflammation	1195:1227	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	0	30	theme	Maternal	0:7	arg1	intake					19:24	Maternal sucralose intake	0:24	Maternal sucralose intake	0:24	Maternal sucralose intake alters gut microbiota of offspring and exacerbates hepatic steatosis in adulthood.					
32228300	8	31	theme	biochemistry	978:989	arg1	severity					941:948	the severity	937:948	the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota	937:1027	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	7	32	theme	barrier	847:853	arg1	function					855:862	mucosal barrier function	839:862	mucosal barrier function	839:862	The intestinal development, mucosal barrier function, and gut microbiota were assessed in the 3-week-old offspring.					
32228300	10	33	theme	intestinal	1195:1204	arg1	inflammation					1216:1227	intestinal low-grade inflammation	1195:1227	intestinal low-grade inflammation	1195:1227	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	12	34	theme	acid	1625:1628	arg1	biosynthesis					1630:1641	disturbed fatty acid biosynthesis	1609:1641	disturbed fatty acid biosynthesis	1609:1641	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	11	35	theme	anti-inflammatory	1450:1466	arg1	effect					1468:1473	the anti-inflammatory effect	1446:1473	the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum	1446:1538	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	8	36	theme	lipid	992:996	arg1	metabolism					998:1007	lipid metabolism	992:1007	lipid metabolism	992:1007	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	1	37	theme	BACKGROUND	109:118	arg1	NAFLD					154:158	NAFLD	154:158	NAFLD	154:158	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	1	37	theme	BACKGROUND	109:118	arg1	disease					145:151	BACKGROUND Nonalcoholic fatty liver disease	109:151	BACKGROUND Nonalcoholic fatty liver disease (NAFLD)	109:159	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	13	38	theme	HFD-induced	1861:1871	arg1	steatosis					1881:1889	HFD-induced hepatic steatosis	1861:1889	HFD-induced hepatic steatosis in adulthood	1861:1902	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	0	39	theme	gut	33:35	arg1	microbiota					37:46	gut microbiota	33:46	gut microbiota of offspring	33:59	Maternal sucralose intake alters gut microbiota of offspring and exacerbates hepatic steatosis in adulthood.					
32228300	4	40	from	steatosis	516:524	arg1	adulthood					529:537	adulthood	529:537	adulthood	529:537	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	10	41	theme	gut	1286:1288	arg1	microbiota					1290:1299	gut microbiota	1286:1299	gut microbiota	1286:1299	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	8	42	theme	gut	1014:1016	arg1	microbiota					1018:1027	gut microbiota	1014:1027	gut microbiota	1014:1027	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	6	43	theme	high-fat	778:785	arg1	HFD					793:795	HFD	793:795	HFD	793:795	After weaning, all offspring were fed a control diet until 8 weeks of age, and then treated with a high-fat diet (HFD) for 4 weeks.					
32228300	6	43	theme	high-fat	778:785	arg1	diet 					787:791	a high-fat diet 	776:791	a high-fat diet (HFD)	776:796	After weaning, all offspring were fed a control diet until 8 weeks of age, and then treated with a high-fat diet (HFD) for 4 weeks.					
32228300	7	44	theme	mucosal	839:845	arg1	function					855:862	mucosal barrier function	839:862	mucosal barrier function	839:862	The intestinal development, mucosal barrier function, and gut microbiota were assessed in the 3-week-old offspring.					
32228300	10	45	theme	microbiota	1290:1299	arg1	diversity					1273:1281	diversity	1273:1281	diversity	1273:1281	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	10	45	theme	microbiota	1290:1299	arg1	production					1367:1376	cecal butyrate production	1352:1376	cecal butyrate production	1352:1376	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	10	45	theme	microbiota	1290:1299	arg1	compositions					1256:1267	compositions	1256:1267	compositions	1256:1267	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	10	45	theme	microbiota	1290:1299	arg1	bacteria					1339:1346	reducing butyrate-producing bacteria	1311:1346	reducing butyrate-producing bacteria	1311:1346	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	10	46	theme	butyrate-producing	1320:1337	arg1	bacteria					1339:1346	reducing butyrate-producing bacteria	1311:1346	reducing butyrate-producing bacteria	1311:1346	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	13	47	from	steatosis	1881:1889	arg1	adulthood					1894:1902	adulthood	1894:1902	adulthood	1894:1902	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	13	48	theme	gut	1784:1786	arg1	dysbiosis					1788:1796	gut dysbiosis	1784:1796	gut dysbiosis	1784:1796	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	5	49	theme	control	649:655	arg1	group					662:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group	575:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet)	575:676	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	5	49	theme	control	649:655	arg1	MC diet					669:675	MC diet	669:675	MC diet	669:675	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	10	50	theme	butyrate	1358:1365	arg1	production					1367:1376	cecal butyrate production	1352:1376	cecal butyrate production	1352:1376	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	2	51	theme	host	288:291	arg1	health					293:298	host health	288:298	host health	288:298	Excessive sucralose can induce gut dysbiosis and negatively affect host health.					
32228300	6	52	theme	age	749:751	arg1	8 weeks					738:744	8 weeks	738:744	8 weeks of age	738:751	After weaning, all offspring were fed a control diet until 8 weeks of age, and then treated with a high-fat diet (HFD) for 4 weeks.					
32228300	13	53	theme	intestinal	1752:1761	arg1	development					1763:1773	intestinal development	1752:1773	intestinal development	1752:1773	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	9	54	theme	disrupted	1127:1135	arg1	function					1145:1152	disrupted barrier function	1127:1152	disrupted barrier function	1127:1152	RESULTS MS significantly inhibited intestinal development and disrupted barrier function in 3-week-old offspring.					
32228300	12	55	theme	disturbed	1609:1617	arg1	biosynthesis					1630:1641	disturbed fatty acid biosynthesis	1609:1641	disturbed fatty acid biosynthesis	1609:1641	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	4	56	theme	maternal	439:446	arg1	intake					463:468	maternal sucralose (MS) intake	439:468	maternal sucralose (MS) intake	439:468	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	13	57	theme	CONCLUSIONS	1721:1731	arg1	intake					1736:1741	CONCLUSIONS MS intake	1721:1741	CONCLUSIONS MS intake	1721:1741	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	9	58	theme	3-week-old	1157:1166	arg1	offspring					1168:1176	3-week-old offspring	1157:1176	3-week-old offspring	1157:1176	RESULTS MS significantly inhibited intestinal development and disrupted barrier function in 3-week-old offspring.					
32228300	4	59	from	effects	428:434	arg1	susceptibility					477:490	the susceptibility	473:490	the susceptibility of offspring to hepatic steatosis in adulthood	473:537	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	1	60	theme	Nonalcoholic	120:131	arg1	NAFLD					154:158	NAFLD	154:158	NAFLD	154:158	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	1	60	theme	Nonalcoholic	120:131	arg1	disease					145:151	BACKGROUND Nonalcoholic fatty liver disease	109:151	BACKGROUND Nonalcoholic fatty liver disease (NAFLD)	109:159	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	12	61	theme	hepatic	1586:1592	arg1	steatosis					1594:1602	hepatic steatosis	1586:1602	hepatic steatosis	1586:1602	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	11	62	theme	one	1478:1480	arg1	effect					1468:1473	the anti-inflammatory effect	1446:1473	the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum	1446:1538	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	5	63	theme	C57BL/6	548:554	arg1	mice					565:568	METHODS C57BL/6 pregnant mice	540:568	METHODS C57BL/6 pregnant mice	540:568	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	1	64	theme	liver	139:143	arg1	NAFLD					154:158	NAFLD	154:158	NAFLD	154:158	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	1	64	theme	liver	139:143	arg1	disease					145:151	BACKGROUND Nonalcoholic fatty liver disease	109:151	BACKGROUND Nonalcoholic fatty liver disease (NAFLD)	109:159	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	11	65	theme	bacteria	1508:1515	arg1	one					1478:1480	one	1478:1480	one	1478:1480	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	11	65	theme	bacteria	1508:1515	arg1	bacteria					1508:1515	the butyrate-producing bacteria	1485:1515	the butyrate-producing bacteria	1485:1515	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	11	65	theme	bacteria	1508:1515	arg1	butyricum					1530:1538	Clostridium butyricum	1518:1538	Clostridium butyricum	1518:1538	Mechanically, blocking GPR43 blunted the anti-inflammatory effect of one of the butyrate-producing bacteria, Clostridium butyricum in vitro.					
32228300	4	66	theme	offspring	495:503	arg1	susceptibility					477:490	the susceptibility	473:490	the susceptibility of offspring to hepatic steatosis in adulthood	473:537	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	0	67	from	steatosis	85:93	arg1	adulthood					98:106	adulthood	98:106	adulthood	98:106	Maternal sucralose intake alters gut microbiota of offspring and exacerbates hepatic steatosis in adulthood.					
32228300	8	68	theme	steatosis	961:969	arg1	severity					941:948	the severity	937:948	the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota	937:1027	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	5	69	theme	group	594:598	arg1	group					662:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group	575:666	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet)	575:676	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	5	69	theme	group	594:598	arg1	MC diet					669:675	MC diet	669:675	MC diet	669:675	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	13	70	theme	hepatic	1873:1879	arg1	steatosis					1881:1889	HFD-induced hepatic steatosis	1861:1889	HFD-induced hepatic steatosis in adulthood	1861:1902	CONCLUSIONS MS intake inhibits intestinal development, induces gut dysbiosis in offspring through down-regulation of GPR43, and exacerbates HFD-induced hepatic steatosis in adulthood.					
32228300	8	71	theme	serum	972:976	arg1	biochemistry					978:989	serum biochemistry	972:989	serum biochemistry	972:989	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	10	72	dep	induced	1187:1193	arg1	changed					1244:1250	changed	1244:1250	changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43	1244:1406	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	3	73	theme	neonate	351:357	arg1	communities					336:346	the microbial communities	322:346	the microbial communities of neonate	322:357	Maternal diet shapes the microbial communities of neonate and this effect continues in later life.					
32228300	0	74	theme	sucralose	9:17	arg1	intake					19:24	Maternal sucralose intake	0:24	Maternal sucralose intake	0:24	Maternal sucralose intake alters gut microbiota of offspring and exacerbates hepatic steatosis in adulthood.					
32228300	3	75	theme	later	388:392	arg1	life					394:397	later life	388:397	later life	388:397	Maternal diet shapes the microbial communities of neonate and this effect continues in later life.					
32228300	8	76	theme	metabolism	998:1007	arg1	severity					941:948	the severity	937:948	the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota	937:1027	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	5	77	theme	randomized	575:584	arg1	group					594:598	randomized into MS group	575:598	randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet)	575:676	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	12	78	theme	fatty	1619:1623	arg1	biosynthesis					1630:1641	disturbed fatty acid biosynthesis	1609:1641	disturbed fatty acid biosynthesis	1609:1641	After HFD treatment, MS exacerbated hepatic steatosis, and disturbed fatty acid biosynthesis and metabolism, accompanied by inducing gut dysbiosis compared with MC group.					
32228300	4	79	theme	hepatic	508:514	arg1	steatosis					516:524	hepatic steatosis	508:524	hepatic steatosis in adulthood	508:537	We aimed to investigate the effects of maternal sucralose (MS) intake on the susceptibility of offspring to hepatic steatosis in adulthood.					
32228300	6	80	theme	control	719:725	arg1	diet					727:730	a control diet	717:730	a control diet until 8 weeks of age	717:751	After weaning, all offspring were fed a control diet until 8 weeks of age, and then treated with a high-fat diet (HFD) for 4 weeks.					
32228300	0	81	theme	offspring	51:59	arg1	microbiota					37:46	gut microbiota	33:46	gut microbiota of offspring	33:59	Maternal sucralose intake alters gut microbiota of offspring and exacerbates hepatic steatosis in adulthood.					
32228300	8	82	theme	microbiota	1018:1027	arg1	severity					941:948	the severity	937:948	the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota	937:1027	Moreover, the severity of hepatic steatosis, serum biochemistry, lipid metabolism, and gut microbiota was then assessed in the 12th week.					
32228300	9	83	theme	RESULTS	1065:1071	arg1	MS					1073:1074	RESULTS MS	1065:1074	RESULTS MS	1065:1074	RESULTS MS significantly inhibited intestinal development and disrupted barrier function in 3-week-old offspring.					
32228300	7	84	theme	intestinal	815:824	arg1	development					826:836	The intestinal development	811:836	The intestinal development	811:836	The intestinal development, mucosal barrier function, and gut microbiota were assessed in the 3-week-old offspring.					
32228300	10	85	theme	cecal	1352:1356	arg1	production					1367:1376	cecal butyrate production	1352:1376	cecal butyrate production	1352:1376	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32228300	5	86	dep	group	594:598	arg1	MS					601:602	MS	601:602	MS	601:602	METHODS C57BL/6 pregnant mice were randomized into MS group (MS during gestation and lactation) and maternal control (MC) group (MC diet).					
32228300	1	87	dep	diet	197:200	arg1	dysbiosis					210:218	dysbiosis	210:218	dysbiosis	210:218	BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is considered to be associated with diet and gut dysbiosis.					
32228300	10	88	theme	reducing	1311:1318	arg1	bacteria					1339:1346	reducing butyrate-producing bacteria	1311:1346	reducing butyrate-producing bacteria	1311:1346	MS also induced intestinal low-grade inflammation, significantly changed the compositions and diversity of gut microbiota including reducing butyrate-producing bacteria and cecal butyrate production with down-regulation of GPR43.					
32422052	11	0	theme	=	1786:1786	arg1	0.51					1788:1791	TS = 78.3 mg/g and MD/RFOs = 0.51	1759:1791	0.51	1788:1791	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	10	1	theme	MD/RFOs	1516:1522	arg1	ratio					1524:1528	MD/RFOs ratio	1516:1528	MD/RFOs ratio	1516:1528	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	8	2	located	observed	1232:1239	arg1	lentils					1269:1275	lentils	1269:1275	lentils (53.6-56.6 mg/g)	1269:1292	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	2	located	observed	1232:1239	arg1	mg/g					1288:1291	53.6-56.6 mg/g	1278:1291	53.6-56.6 mg/g	1278:1291	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	2	located	observed	1232:1239	arg1	peas					1260:1263	speckled butter peas	1244:1263	speckled butter peas	1244:1263	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	2	located	observed	1232:1239	arg2	amounts					1219:1225	lower amounts	1213:1225	lower amounts	1213:1225	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	3	contain	contained	1138:1146	arg2	amounts					1155:1161	higher amounts	1148:1161	higher amounts of total soluble sugars (79.8-83.6 mg/g)	1148:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	3	contain	contained	1138:1146	arg2	sugars					1180:1185	total soluble sugars	1166:1185	total soluble sugars (79.8-83.6 mg/g)	1166:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	3	contain	contained	1138:1146	arg2	mg/g					1198:1201	79.8-83.6 mg/g	1188:1201	79.8-83.6 mg/g	1188:1201	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	3	contain	contained	1138:1146	arg1	peas					1133:1136	black eyed peas	1122:1136	black eyed peas	1122:1136	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	3	contain	contained	1138:1146	arg1	beans					1099:1103	Yellow split beans	1086:1103	Yellow split beans	1086:1103	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	3	contain	contained	1138:1146	arg1	lima					1112:1115	large lima	1106:1115	large lima	1106:1115	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	11	4	dep	=	1717:1717	arg1	mg/g					1724:1727	77.6 mg/g	1719:1727	77.6 mg/g	1719:1727	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	4	dep	=	1717:1717	arg1	MD/RFOs					1733:1739	MD/RFOs	1733:1739	MD/RFOs	1733:1739	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	12	5	theme	flatulence	2062:2071	arg1	problems					2073:2080	reduced flatulence problems	2054:2080	reduced flatulence problems	2054:2080	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	8	6	theme	black	1122:1126	arg1	peas					1133:1136	black eyed peas	1122:1136	black eyed peas	1122:1136	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	3	7	theme	analytical	452:461	arg1	method					463:468	a newly developed and validated analytical method	420:468	a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection	420:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	8	8	theme	Yellow	1086:1091	arg1	beans					1099:1103	Yellow split beans	1086:1103	Yellow split beans	1086:1103	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	11	9	theme	TS	1588:1589	arg1	content					1591:1597	average TS content	1580:1597	average TS content	1580:1597	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	9	theme	TS	1588:1589	arg1	group					1795:1799	group 4	1795:1801	group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06)	1795:1837	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	9	theme	TS	1588:1589	arg1	group					1750:1754	group 3	1750:1756	group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51)	1750:1792	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	9	theme	TS	1588:1589	arg1	group					1705:1709	group 2	1705:1711	group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31)	1705:1747	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	9	theme	TS	1588:1589	arg1	group					1844:1848	group 5	1844:1850	group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62)	1844:1886	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	9	theme	TS	1588:1589	arg1	ratios					1615:1620	the MD/RFOs ratios	1603:1620	the MD/RFOs ratios	1603:1620	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	9	theme	TS	1588:1589	arg1	group					1660:1664	group 1	1660:1666	group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30)	1660:1702	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	7	10	theme	prominent	990:998	arg1	stachyose					967:975	stachyose	967:975	stachyose	967:975	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	7	10	theme	prominent	990:998	arg1	sugars					1008:1013	the two prominent soluble sugars	982:1013	the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars	982:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	7	10	theme	prominent	990:998	arg1	Sucrose					955:961	Sucrose	955:961	Sucrose	955:961	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	9	11	theme	maximum	1320:1326	arg1	levels					1328:1333	maximum levels	1320:1333	maximum levels of mono and disaccharides (MD)	1320:1364	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	9	12	theme	Garbanzo	1295:1302	arg1	beans					1304:1308	Garbanzo beans	1295:1308	Garbanzo beans	1295:1308	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	12	13	theme	animal	2033:2038	arg1	proteins					2040:2047	animal proteins	2033:2047	animal proteins with reduced flatulence problems	2033:2080	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	8	14	theme	higher	1148:1153	arg1	sugars					1180:1185	total soluble sugars	1166:1185	total soluble sugars (79.8-83.6 mg/g)	1166:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	14	theme	higher	1148:1153	arg1	mg/g					1198:1201	79.8-83.6 mg/g	1188:1201	79.8-83.6 mg/g	1188:1201	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	14	theme	higher	1148:1153	arg1	amounts					1155:1161	higher amounts	1148:1161	higher amounts of total soluble sugars (79.8-83.6 mg/g)	1148:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	3	15	theme	anion-exchange	492:505	arg1	chromatography					507:520	high-performance anion-exchange chromatography	475:520	high-performance anion-exchange chromatography coupled to an amperometric pulse detection	475:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	8	16	theme	large	1106:1110	arg1	lima					1112:1115	large lima	1106:1115	large lima	1106:1115	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	2	17	dep	oligosaccharides	244:259	arg1	stachyose					279:287	stachyose	279:287	stachyose	279:287	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	2	17	dep	oligosaccharides	244:259	arg1	verbascose					294:303	verbascose	294:303	verbascose	294:303	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	2	17	dep	oligosaccharides	244:259	arg1	raffinose					268:276	raffinose	268:276	raffinose	268:276	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	2	17	dep	oligosaccharides	244:259	arg1	RFOs					262:265	RFOs	262:265	RFOs	262:265	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	2	17	dep	oligosaccharides	244:259	arg1	oligosaccharides					244:259	oligosaccharides	244:259	oligosaccharides (RFOs: raffinose, stachyose, and verbascose)	244:304	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	11	18	theme	=	1880:1880	arg1	0.62					1882:1885	TS = 68.5 mg/g and MD/RFOs = 0.62	1853:1885	0.62	1882:1885	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	content					1591:1597	average TS content	1580:1597	average TS content	1580:1597	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	group					1795:1799	group 4	1795:1801	group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06)	1795:1837	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	group					1750:1754	group 3	1750:1756	group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51)	1750:1792	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	group					1705:1709	group 2	1705:1711	group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31)	1705:1747	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	group					1844:1848	group 5	1844:1850	group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62)	1844:1886	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	ratios					1615:1620	the MD/RFOs ratios	1603:1620	the MD/RFOs ratios	1603:1620	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	19	theme	MD/RFOs	1607:1613	arg1	group					1660:1664	group 1	1660:1666	group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30)	1660:1702	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	3	20	theme	pulse	549:553	arg1	detection					555:563	an amperometric pulse detection	533:563	an amperometric pulse detection	533:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	11	21	theme	=	1741:1741	arg1	mg/g					1724:1727	77.6 mg/g	1719:1727	77.6 mg/g	1719:1727	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	8	22	theme	soluble	1172:1178	arg1	sugars					1180:1185	total soluble sugars	1166:1185	total soluble sugars (79.8-83.6 mg/g)	1166:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	22	theme	soluble	1172:1178	arg1	mg/g					1198:1201	79.8-83.6 mg/g	1188:1201	79.8-83.6 mg/g	1188:1201	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	10	23	theme	cluster	1451:1457	arg1	analysis					1459:1466	the hierarchical cluster analysis	1434:1466	the hierarchical cluster analysis of the total soluble sugars (TS)	1434:1499	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	6	24	theme	Significant	835:845	arg1	variations					847:856	Significant variations	835:856	Significant variations in the profiles and concentrations of individual and total sugars	835:922	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	11	25	theme	TS	1759:1760	arg1	mg/g					1769:1772	TS = 78.3 mg/g and MD/RFOs = 0.51	1759:1791	mg/g	1769:1772	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	6	26	dep	profiles	865:872	arg1	the					861:863	the	861:863	the	861:863	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	4	27	theme	good	668:671	arg1	linearity					673:681	good linearity	668:681	good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL	668:720	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	27	theme	good	668:671	arg1	≥					687:687	r2 ≥ 0.99	684:692	r2 ≥ 0.99	684:692	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	12	28	with	proteins	2040:2047	arg1	problems					2073:2080	reduced flatulence problems	2054:2080	reduced flatulence problems	2054:2080	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	0	29	theme	Oligosaccharide	39:53	arg1	Content					55:61	Soluble Mono, Di, and Oligosaccharide Content	17:61	Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.)	17:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	7	30	theme	total	1064:1068	arg1	sugars					1078:1083	the total soluble sugars	1060:1083	the total soluble sugars	1060:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	11	31	dep	=	1741:1741	arg1	0.31					1743:1746	0.31	1743:1746	0.31	1743:1746	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	0	32	dep	vulgaris	90:97	arg1	L					99:99	L	99:99	Phaseolus vulgaris L.	80:100	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	6	33	theme	total	911:915	arg1	sugars					917:922	individual and total sugars	896:922	individual and total sugars	896:922	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	0	34	from	Determination	0:12	arg1	Beans					73:77	23 Dry Beans	66:77	23 Dry Beans (Phaseolus vulgaris L.)	66:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	11	35	dep	=	1807:1807	arg1	MD/RFOs					1823:1829	MD/RFOs	1823:1829	MD/RFOs	1823:1829	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	35	dep	=	1807:1807	arg1	mg/g					1814:1817	59.1 mg/g	1809:1817	59.1 mg/g	1809:1817	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	4	36	theme	r2	684:685	arg1	linearity					673:681	good linearity	668:681	good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL	668:720	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	36	theme	r2	684:685	arg1	≥					687:687	r2 ≥ 0.99	684:692	r2 ≥ 0.99	684:692	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	6	37	located	observed	929:936	arg1	beans					948:952	23 dry beans	941:952	23 dry beans	941:952	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	6	37	located	observed	929:936	arg2	variations					847:856	Significant variations	835:856	Significant variations in the profiles and concentrations of individual and total sugars	835:922	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	0	38	theme	Dry	69:71	arg1	Beans					73:77	23 Dry Beans	66:77	23 Dry Beans (Phaseolus vulgaris L.)	66:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	6	39	theme	individual	896:905	arg1	sugars					917:922	individual and total sugars	896:922	individual and total sugars	896:922	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	3	40	theme	soluble	359:365	arg1	content					373:379	the soluble sugar content	355:379	the soluble sugar content of 23 dry beans	355:395	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	0	41	theme	Di	31:32	arg1	Content					55:61	Soluble Mono, Di, and Oligosaccharide Content	17:61	Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.)	17:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	9	42	theme	highest	1401:1407	arg1	levels					1409:1414	the highest levels	1397:1414	the highest levels of RFOs	1397:1422	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	11	43	theme	=	1807:1807	arg1	TS					1804:1805	TS	1804:1805	TS = 59.1 mg/g and MD/RFOs = 1.06	1804:1836	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	10	44	theme	soluble	1481:1487	arg1	sugars					1489:1494	the total soluble sugars	1471:1494	the total soluble sugars (TS)	1471:1499	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	10	44	theme	soluble	1481:1487	arg1	TS					1497:1498	TS	1497:1498	TS	1497:1498	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	7	45	theme	sugars	1078:1083	arg1	%					1055:1055	86%	1053:1055	86% of the total soluble sugars	1053:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	7	45	theme	sugars	1078:1083	arg1	sugars					1078:1083	the total soluble sugars	1060:1083	the total soluble sugars	1060:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	12	46	theme	reduced	2054:2060	arg1	problems					2073:2080	reduced flatulence problems	2054:2080	reduced flatulence problems	2054:2080	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	11	47	dep	=	1696:1696	arg1	0.30					1698:1701	0.30	1698:1701	0.30	1698:1701	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	8	48	theme	butter	1253:1258	arg1	peas					1260:1263	speckled butter peas	1244:1263	speckled butter peas	1244:1263	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	0	49	theme	Soluble	17:23	arg1	Content					55:61	Soluble Mono, Di, and Oligosaccharide Content	17:61	Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.)	17:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	2	50	theme	raffinose	224:232	arg1	family					234:239	the raffinose family	220:239	the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose)	220:304	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	11	51	dep	group	1660:1664	arg1	TS					1669:1670	TS	1669:1670	TS = 55.1 mg/g and MD/RFOs = 0.30	1669:1701	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	1	52	theme	rich	120:123	arg1	source					125:130	a rich source	118:130	a rich source of plant-based proteins and carbohydrates	118:172	Beans provide a rich source of plant-based proteins and carbohydrates.					
32422052	6	53	theme	dry	944:946	arg1	beans					948:952	23 dry beans	941:952	23 dry beans	941:952	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	6	54	from	variations	847:856	arg1	concentrations					878:891	concentrations	878:891	concentrations	878:891	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	6	54	from	variations	847:856	arg1	profiles					865:872	profiles	865:872	profiles	865:872	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	4	55	dep	sugars	576:581	arg1	sugars					576:581	All seven sugars	566:581	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose)	566:659	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	verbascose					649:658	verbascose	649:658	verbascose	649:658	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	galactose					584:592	galactose	584:592	galactose	584:592	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	sucrose					614:620	sucrose	614:620	sucrose	614:620	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	raffinose					623:631	raffinose	623:631	raffinose	623:631	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	glucose					595:601	glucose	595:601	glucose	595:601	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	fructose					604:611	fructose	604:611	fructose	604:611	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	4	55	dep	sugars	576:581	arg1	stachyose					634:642	stachyose	634:642	stachyose	634:642	All seven sugars (galactose, glucose, fructose, sucrose, raffinose, stachyose, and verbascose) showed good linearity (r2 ≥ 0.99) between 0.156 and 20 μg/mL.					
32422052	12	56	theme	protein	1997:2003	arg1	source					2005:2010	plant-based protein source	1985:2010	plant-based protein source	1985:2010	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	3	57	theme	beans	391:395	arg1	content					373:379	the soluble sugar content	355:379	the soluble sugar content of 23 dry beans	355:395	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	3	58	theme	developed	428:436	arg1	method					463:468	a newly developed and validated analytical method	420:468	a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection	420:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	11	59	dep	group	1844:1848	arg1	mg/g					1863:1866	TS = 68.5 mg/g and MD/RFOs = 0.62	1853:1885	mg/g	1863:1866	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	59	dep	group	1844:1848	arg1	0.62					1882:1885	TS = 68.5 mg/g and MD/RFOs = 0.62	1853:1885	0.62	1882:1885	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	12	60	theme	food	1933:1936	arg1	industries					1938:1947	food industries	1933:1947	food industries	1933:1947	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	11	61	theme	=	1672:1672	arg1	TS					1669:1670	TS	1669:1670	TS = 55.1 mg/g and MD/RFOs = 0.30	1669:1701	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	62	theme	MD/RFOs	1778:1784	arg1	0.51					1788:1791	TS = 78.3 mg/g and MD/RFOs = 0.51	1759:1791	0.51	1788:1791	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	7	63	theme	soluble	1000:1006	arg1	stachyose					967:975	stachyose	967:975	stachyose	967:975	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	7	63	theme	soluble	1000:1006	arg1	sugars					1008:1013	the two prominent soluble sugars	982:1013	the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars	982:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	7	63	theme	soluble	1000:1006	arg1	Sucrose					955:961	Sucrose	955:961	Sucrose	955:961	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	3	64	theme	validated	442:450	arg1	method					463:468	a newly developed and validated analytical method	420:468	a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection	420:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	5	65	theme	quantification	750:763	arg1	limit					727:731	The limit	723:731	The limit of detection and quantification	723:763	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	5	65	theme	quantification	750:763	arg1	μg/mL					794:798	0.01-0.11 μg/mL	784:798	0.01-0.11 μg/mL	784:798	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	5	65	theme	quantification	750:763	arg1	μg/mL					814:818	0.04-0.32 μg/mL	804:818	0.04-0.32 μg/mL	804:818	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	11	66	theme	=	1856:1856	arg1	mg/g					1863:1866	TS = 68.5 mg/g and MD/RFOs = 0.62	1853:1885	mg/g	1863:1866	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	10	67	theme	hierarchical	1438:1449	arg1	analysis					1459:1466	the hierarchical cluster analysis	1434:1466	the hierarchical cluster analysis of the total soluble sugars (TS)	1434:1499	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	8	68	theme	eyed	1128:1131	arg1	peas					1133:1136	black eyed peas	1122:1136	black eyed peas	1122:1136	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	11	69	dep	=	1672:1672	arg1	mg/g					1679:1682	55.1 mg/g	1674:1682	55.1 mg/g	1674:1682	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	69	dep	=	1672:1672	arg1	MD/RFOs					1688:1694	MD/RFOs	1688:1694	MD/RFOs	1688:1694	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	3	70	theme	high-performance	475:490	arg1	chromatography					507:520	high-performance anion-exchange chromatography	475:520	high-performance anion-exchange chromatography coupled to an amperometric pulse detection	475:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	1	71	theme	proteins	147:154	arg1	source					125:130	a rich source	118:130	a rich source of plant-based proteins and carbohydrates	118:172	Beans provide a rich source of plant-based proteins and carbohydrates.					
32422052	8	72	theme	split	1093:1097	arg1	beans					1099:1103	Yellow split beans	1086:1103	Yellow split beans	1086:1103	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	5	73	theme	0.04-0.32	804:812	arg1	limit					727:731	The limit	723:731	The limit of detection and quantification	723:763	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	5	73	theme	0.04-0.32	804:812	arg1	μg/mL					814:818	0.04-0.32 μg/mL	804:818	0.04-0.32 μg/mL	804:818	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	9	74	theme	split	1378:1382	arg1	beans					1384:1388	yellow split beans	1371:1388	yellow split beans	1371:1388	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	11	75	theme	average	1580:1586	arg1	content					1591:1597	average TS content	1580:1597	average TS content	1580:1597	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	75	theme	average	1580:1586	arg1	group					1795:1799	group 4	1795:1801	group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06)	1795:1837	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	75	theme	average	1580:1586	arg1	group					1750:1754	group 3	1750:1756	group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51)	1750:1792	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	75	theme	average	1580:1586	arg1	group					1705:1709	group 2	1705:1711	group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31)	1705:1747	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	75	theme	average	1580:1586	arg1	group					1844:1848	group 5	1844:1850	group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62)	1844:1886	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	75	theme	average	1580:1586	arg1	ratios					1615:1620	the MD/RFOs ratios	1603:1620	the MD/RFOs ratios	1603:1620	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	75	theme	average	1580:1586	arg1	group					1660:1664	group 1	1660:1666	group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30)	1660:1702	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	3	76	theme	amperometric	536:547	arg1	detection					555:563	an amperometric pulse detection	533:563	an amperometric pulse detection	533:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	1	77	theme	plant-based	135:145	arg1	proteins					147:154	plant-based proteins	135:154	plant-based proteins	135:154	Beans provide a rich source of plant-based proteins and carbohydrates.					
32422052	5	78	theme	detection	736:744	arg1	limit					727:731	The limit	723:731	The limit of detection and quantification	723:763	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	5	78	theme	detection	736:744	arg1	μg/mL					794:798	0.01-0.11 μg/mL	784:798	0.01-0.11 μg/mL	784:798	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	5	78	theme	detection	736:744	arg1	μg/mL					814:818	0.04-0.32 μg/mL	804:818	0.04-0.32 μg/mL	804:818	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	11	79	theme	=	1717:1717	arg1	TS					1714:1715	TS	1714:1715	TS = 77.6 mg/g and MD/RFOs = 0.31	1714:1746	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	0	80	from	Content	55:61	arg1	Beans					73:77	23 Dry Beans	66:77	23 Dry Beans (Phaseolus vulgaris L.)	66:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	11	81	dep	group	1795:1799	arg1	TS					1804:1805	TS	1804:1805	TS = 59.1 mg/g and MD/RFOs = 1.06	1804:1836	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	82	theme	=	1696:1696	arg1	mg/g					1679:1682	55.1 mg/g	1674:1682	55.1 mg/g	1674:1682	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	9	83	theme	disaccharides	1347:1359	arg1	levels					1328:1333	maximum levels	1320:1333	maximum levels of mono and disaccharides (MD)	1320:1364	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	8	84	theme	total	1166:1170	arg1	sugars					1180:1185	total soluble sugars	1166:1185	total soluble sugars (79.8-83.6 mg/g)	1166:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	84	theme	total	1166:1170	arg1	mg/g					1198:1201	79.8-83.6 mg/g	1188:1201	79.8-83.6 mg/g	1188:1201	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	11	85	theme	=	1762:1762	arg1	mg/g					1769:1772	TS = 78.3 mg/g and MD/RFOs = 0.51	1759:1791	mg/g	1769:1772	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	0	86	from	Beans	73:77	arg1	Determination					0:12	Determination	0:12	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).	0:102	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	8	87	theme	lower	1213:1217	arg1	amounts					1219:1225	lower amounts	1213:1225	lower amounts	1213:1225	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	88	theme	sugars	1180:1185	arg1	sugars					1180:1185	total soluble sugars	1166:1185	total soluble sugars (79.8-83.6 mg/g)	1166:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	88	theme	sugars	1180:1185	arg1	mg/g					1198:1201	79.8-83.6 mg/g	1188:1201	79.8-83.6 mg/g	1188:1201	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	8	88	theme	sugars	1180:1185	arg1	amounts					1155:1161	higher amounts	1148:1161	higher amounts of total soluble sugars (79.8-83.6 mg/g)	1148:1202	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	3	89	theme	sugar	367:371	arg1	content					373:379	the soluble sugar content	355:379	the soluble sugar content of 23 dry beans	355:395	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	0	90	theme	Content	55:61	arg1	Determination					0:12	Determination	0:12	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).	0:102	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	11	91	dep	group	1705:1709	arg1	TS					1714:1715	TS	1714:1715	TS = 77.6 mg/g and MD/RFOs = 0.31	1714:1746	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	5	92	theme	0.01-0.11	784:792	arg1	limit					727:731	The limit	723:731	The limit of detection and quantification	723:763	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	5	92	theme	0.01-0.11	784:792	arg1	μg/mL					794:798	0.01-0.11 μg/mL	784:798	0.01-0.11 μg/mL	784:798	The limit of detection and quantification were determined as 0.01-0.11 μg/mL and 0.04-0.32 μg/mL, respectively.					
32422052	9	93	theme	yellow	1371:1376	arg1	beans					1384:1388	yellow split beans	1371:1388	yellow split beans	1371:1388	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	6	94	theme	sugars	917:922	arg1	concentrations					878:891	concentrations	878:891	concentrations	878:891	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	6	94	theme	sugars	917:922	arg1	profiles					865:872	profiles	865:872	profiles	865:872	Significant variations in the profiles and concentrations of individual and total sugars were observed in 23 dry beans.					
32422052	9	95	contain	contained	1310:1318	arg1	beans					1304:1308	Garbanzo beans	1295:1308	Garbanzo beans	1295:1308	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	9	95	contain	contained	1310:1318	arg2	levels					1328:1333	maximum levels	1320:1333	maximum levels of mono and disaccharides (MD)	1320:1364	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	11	96	dep	content	1591:1597	arg1	The					1576:1578	The	1576:1578	The	1576:1578	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	2	97	theme	oligosaccharides	244:259	arg1	family					234:239	the raffinose family	220:239	the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose)	220:304	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	11	98	theme	TS	1853:1854	arg1	mg/g					1863:1866	TS = 68.5 mg/g and MD/RFOs = 0.62	1853:1885	mg/g	1863:1866	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	99	theme	MD/RFOs	1872:1878	arg1	0.62					1882:1885	TS = 68.5 mg/g and MD/RFOs = 0.62	1853:1885	0.62	1882:1885	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	0	100	theme	Phaseolus	80:88	arg1	vulgaris					90:97	Phaseolus vulgaris	80:97	Phaseolus vulgaris L.	80:100	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	0	101	dep	Beans	73:77	arg1	vulgaris					90:97	Phaseolus vulgaris	80:97	Phaseolus vulgaris L.	80:100	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	10	102	theme	sugars	1489:1494	arg1	analysis					1459:1466	the hierarchical cluster analysis	1434:1466	the hierarchical cluster analysis of the total soluble sugars (TS)	1434:1499	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	10	102	theme	sugars	1489:1494	arg1	MD					1502:1503	MD	1502:1503	MD	1502:1503	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	10	102	theme	sugars	1489:1494	arg1	RFOs					1506:1509	RFOs	1506:1509	RFOs	1506:1509	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	10	102	theme	sugars	1489:1494	arg1	ratio					1524:1528	MD/RFOs ratio	1516:1528	MD/RFOs ratio	1516:1528	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	7	103	theme	soluble	1070:1076	arg1	sugars					1078:1083	the total soluble sugars	1060:1083	the total soluble sugars	1060:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	11	104	dep	=	1831:1831	arg1	1.06					1833:1836	1.06	1833:1836	1.06	1833:1836	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	10	105	theme	total	1475:1479	arg1	sugars					1489:1494	the total soluble sugars	1471:1494	the total soluble sugars (TS)	1471:1499	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	10	105	theme	total	1475:1479	arg1	TS					1497:1498	TS	1497:1498	TS	1497:1498	Based on the hierarchical cluster analysis of the total soluble sugars (TS), MD, RFOs, and MD/RFOs ratio, 23 beans can be classified into five groups.					
32422052	0	106	theme	Mono	25:28	arg1	Content					55:61	Soluble Mono, Di, and Oligosaccharide Content	17:61	Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.)	17:101	Determination of Soluble Mono, Di, and Oligosaccharide Content in 23 Dry Beans (Phaseolus vulgaris L.).					
32422052	9	107	theme	RFOs	1419:1422	arg1	levels					1409:1414	the highest levels	1397:1414	the highest levels of RFOs	1397:1422	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	8	108	theme	speckled	1244:1251	arg1	peas					1260:1263	speckled butter peas	1244:1263	speckled butter peas	1244:1263	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	3	109	theme	dry	387:389	arg1	beans					391:395	23 dry beans	384:395	23 dry beans	384:395	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	7	110	theme	%	1055:1055	arg1	average					1042:1048	an average	1039:1048	an average of 86% of the total soluble sugars	1039:1083	Sucrose and stachyose were the two prominent soluble sugars combinedly representing an average of 86% of the total soluble sugars.					
32422052	1	111	theme	carbohydrates	160:172	arg1	source					125:130	a rich source	118:130	a rich source of plant-based proteins and carbohydrates	118:172	Beans provide a rich source of plant-based proteins and carbohydrates.					
32422052	11	112	theme	=	1831:1831	arg1	mg/g					1814:1817	59.1 mg/g	1809:1817	59.1 mg/g	1809:1817	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	12	113	theme	plant-based	1985:1995	arg1	source					2005:2010	plant-based protein source	1985:2010	plant-based protein source	1985:2010	This information is useful for researchers, food industries, and consumers that are looking for plant-based protein source as an alternative to animal proteins with reduced flatulence problems.					
32422052	3	114	with	method	463:468	arg1	chromatography					507:520	high-performance anion-exchange chromatography	475:520	high-performance anion-exchange chromatography coupled to an amperometric pulse detection	475:563	In this study, the soluble sugar content of 23 dry beans was investigated using a newly developed and validated analytical method with high-performance anion-exchange chromatography coupled to an amperometric pulse detection.					
32422052	9	115	theme	mono	1338:1341	arg1	levels					1328:1333	maximum levels	1320:1333	maximum levels of mono and disaccharides (MD)	1320:1364	Garbanzo beans contained maximum levels of mono and disaccharides (MD), and yellow split beans showed the highest levels of RFOs.					
32422052	11	116	theme	groups	1634:1639	arg1	content					1591:1597	average TS content	1580:1597	average TS content	1580:1597	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	116	theme	groups	1634:1639	arg1	group					1795:1799	group 4	1795:1801	group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06)	1795:1837	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	116	theme	groups	1634:1639	arg1	group					1750:1754	group 3	1750:1756	group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51)	1750:1792	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	116	theme	groups	1634:1639	arg1	group					1705:1709	group 2	1705:1711	group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31)	1705:1747	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	116	theme	groups	1634:1639	arg1	group					1844:1848	group 5	1844:1850	group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62)	1844:1886	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	116	theme	groups	1634:1639	arg1	ratios					1615:1620	the MD/RFOs ratios	1603:1620	the MD/RFOs ratios	1603:1620	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	116	theme	groups	1634:1639	arg1	group					1660:1664	group 1	1660:1666	group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30)	1660:1702	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	8	117	dep	contained	1138:1146	arg1	whereas					1205:1211	whereas	1205:1211	whereas	1205:1211	Yellow split beans, large lima, and black eyed peas contained higher amounts of total soluble sugars (79.8-83.6 mg/g), whereas lower amounts were observed in speckled butter peas and lentils (53.6-56.6 mg/g).					
32422052	2	118	theme	flatulence	321:330	arg1	issues					332:337	flatulence issues	321:337	flatulence issues	321:337	It is well documented in the literature that the raffinose family of oligosaccharides (RFOs: raffinose, stachyose, and verbascose) is linked with flatulence issues.					
32422052	11	119	dep	group	1750:1754	arg1	0.51					1788:1791	TS = 78.3 mg/g and MD/RFOs = 0.51	1759:1791	0.51	1788:1791	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).					
32422052	11	119	dep	group	1750:1754	arg1	mg/g					1769:1772	TS = 78.3 mg/g and MD/RFOs = 0.51	1759:1791	mg/g	1769:1772	The average TS content and the MD/RFOs ratios of the five groups were determined as group 1 (TS = 55.1 mg/g and MD/RFOs = 0.30), group 2 (TS = 77.6 mg/g and MD/RFOs = 0.31), group 3 (TS = 78.3 mg/g and MD/RFOs = 0.51), group 4 (TS = 59.1 mg/g and MD/RFOs = 1.06), and group 5 (TS = 68.5 mg/g and MD/RFOs = 0.62).